LOCUS       DASRJA010000001         3773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1683192,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000001 DASRJA010000000
VERSION     DASRJA010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683192"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="VFC01_00005"
     CDS             complement(<1..160)
                     /locus_tag="VFC01_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN78061.1"
                     /translation="MFGDVVRRHRRRLGLSQQDLADKSGVTVRGLRKIESGRIGTPRP
                     VTVRLLADA"
     gene            642..1811
                     /locus_tag="VFC01_00010"
     CDS             642..1811
                     /locus_tag="VFC01_00010"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HZN78062.1"
                     /translation="MARQAPYMELDQKVRALAAIDVSPLAGTRLDVAGGRLFVYWAGD
                     PPASLTALRQSAQRSGISLVVERARFSERQLKDAANVLGTFAEAQKLELRTSVHIDGS
                     GLTVEARGLATAAAGSTAARTTAQSQVLAAIDAVRGRTGIPVRVADASDAQPQPANRN
                     SDWTPFWAGAQTDSANTFLDPDCTSGFSLYNTGNATRYMLTAAHCTGWADDIVVENGV
                     NQRMGVSDFIHELFDIRADRYDLGVIRLDAGLSNQGRAYSQWTIANGLYVKGYAAGGI
                     PANGNYCVSGAVSVPNCNLLSGGAVHICSFVPFGRCVWYISFTSINEGIVACLGDSGG
                     PVYYHQGIPTQVIAAGAVGFLNLPPGQGQNCGTTGGVSVVATAINLIPGLGVVTS"
     gene            2280..3410
                     /locus_tag="VFC01_00015"
     CDS             2280..3410
                     /locus_tag="VFC01_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78063.1"
                     /translation="MSRHLKHAAVVVAAVGALLLTGSPAHALGWATVPSPNEVPGNNY
                     LHGADASDAGDVWAVGGFLAPRGGPSHGLVLRYDGMAWGSVPRTGLPGNESLKGVDAV
                     SANDVWVVGGHHASISRNDTLAARWNGTTWTREPTPNGNPVGFNHLNGVAAAGGTVWA
                     VGTYVDPNSSINRRKLILQRAGGSWRVSAAPTVATYEHLAAVDATGLADAWAVGSATS
                     NIQSAPLVPLALRWNGTSWASMTLPATASTALTGVDARTPSDVWVAGSSSNADGTQPY
                     VARFDGTSWRRMATPTIPGGGQLSDVVALSPSAVVAVGRSNGAPLVLRWDGTSWTRES
                     TPASSNPYLTGAAAAGPNAVWAVGYRFDLNAYSNRTLTMLGT"
     gene            complement(3550..>3773)
                     /locus_tag="VFC01_00020"
     CDS             complement(3550..>3773)
                     /locus_tag="VFC01_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78064.1"
                     /translation="DVSPTVGRAQFLGAWRVCADMGIGGGPLLIGALTAAASLTAAAV
                     AIGGLGLAAAWLLHRWLDNRPGSALTLNR"
BASE COUNT          654 a         1291 c         1216 g          612 t
ORIGIN      
        1 acgcgtcggc gagcagccgc acggtcaccg gtcgcggcgt gccgatccga cccgactcga
       61 tcttgcgtag cccgcgcacc gtgacaccac tcttgtccgc cagatcttgc tggctgaggc
      121 cgagcctgcg ccgatgccga cgcacgacgt ctccgaacac tgcccgtcac ccccgtcagg
      181 tccatctcat cgtgcccgcc caaagtacct gtgaagttcc ggttccgtcc ctcccctaca
      241 tcgacacaca tttgccaccc tggcggcagg aagcgcaacg ggattcggta tcgcgcccgg
      301 cgacgtgaca atcggcaatg acaagcaccc agctaacgcg gcagtgcccg acagctgagc
      361 gcctcgtcct gacatttagg gagatgaata gcaattcgag aagcactcat gacatcgaga
      421 atgagtattc ggaccatgcc agcagcgggg ttcgattagg gcccgttcaa ttgtatcgac
      481 ctggggagtt gtatatgtac ctgagactga ttcggaacag aatcgccgtg atatcgatcg
      541 cttgtgcggt gctgccgatg gccgtttcta ccgtccccgc caccgtcctc gcatcatctg
      601 cgactggctc accaccgagc agtgtggcag ggaacgagtt gatggcacgg caggcgccct
      661 acatggaact cgatcagaag gtccgcgcac tggccgcgat cgacgtgtcg cctcttgccg
      721 gcacacgcct cgatgtggcg ggtggccgac tttttgtcta ttgggcaggc gatccgccgg
      781 cgagcctgac ggcgctgcgt caatcggcgc aaaggtcggg catctcactc gtcgtcgaac
      841 gcgcacgctt ctcggaacgg cagctcaagg acgcggctaa cgtgctgggc acattcgccg
      901 aagcgcagaa gctggagctg cgcaccagtg tccacatcga tggttccggg ctcacggtcg
      961 aggcacgtgg cctggcgacg gccgccgccg gatccactgc ggcccgcacg acggcgcagt
     1021 cacaggtcct ggccgccata gatgccgtac ggggccggac cggcattccg gtccgggtcg
     1081 cagacgcatc agatgcgcaa ccgcagccag ccaaccggaa cagcgactgg acccccttct
     1141 gggctggcgc ccagaccgac agcgctaaca catttttgga tcccgactgt accagcgggt
     1201 tcagcctata caacacgggc aacgcgaccc ggtacatgct gactgctgct cactgcaccg
     1261 ggtgggcgga cgacatcgtc gtcgagaacg gtgtgaacca gcggatggga gtctccgact
     1321 tcatccacga gctgttcgac atccgcgccg accgctacga cctaggtgtg atccggctcg
     1381 atgccggact gagcaaccag gggagagcct atagccagtg gaccatcgcc aacggtctct
     1441 acgtcaaagg ttatgcggca ggcggcatac cggccaacgg caactactgc gtctccggcg
     1501 ccgtatcggt accgaactgc aacctgctct caggcggcgc ggtccatatt tgtagcttcg
     1561 tccctttcgg tcgctgcgtc tggtatataa gtttcacgag catcaatgaa gggattgtgg
     1621 catgcttggg tgacagtggt gggccggtct actaccacca gggtattcct acgcaggtga
     1681 tcgccgcagg cgcggtcggc ttcttgaacc taccgccagg acaaggacag aactgcggta
     1741 cgacgggcgg cgtttcggta gtggccaccg cgatcaacct cattcctggg cttggtgtcg
     1801 tcacgagttg acgaaccacc aatagcagcc gcccggggac gatggtcccc gggcgaccac
     1861 ccactcggag cgatcgcgta cttggtgtcc gcatcactga tgcctatccg gatctggtta
     1921 agacctcctc tcttggggat cgacggatgg cgaagccgta ccgcaggaat cgctggccga
     1981 ggctttggtc accgtccgta ctggctgcat gcggcggccg tcctccgcca gcggcaccgg
     2041 gcccgatcat ttcggctggt caccctgcgg gcggccgtgc gggcaggtgt cgcaagggcc
     2101 tccagcgtcg tggtctgcgc tatgcgcggg gtgtagatcg ccgccgtccg cgaccgctga
     2161 gccgatccaa ggattgttca aggttttatg ccatgtcgat cggccaggat cgttcatcgg
     2221 ggtactggcg cgacagcccc gaactccgcg acggctgcgc aacttgatca ggggagccga
     2281 tgtctcgaca tctcaagcac gcggcagttg tcgtggcggc cgtcggtgca ctgctgctca
     2341 ccggcagccc cgcacacgca ctgggctggg ccaccgttcc cagtcccaac gaggtgccgg
     2401 gcaacaacta cctgcacggc gcggacgcgt cggacgccgg tgacgtctgg gccgtggggg
     2461 gtttcttggc cccgagaggc ggcccctcgc acggcctggt gttgcggtac gacgggatgg
     2521 cctggggctc cgtgccgcgc acgggcctac cgggcaacga gagcctcaag ggcgtcgatg
     2581 cggtctccgc caatgacgtc tgggtggtcg gcggtcacca cgccagcatc agccgaaacg
     2641 acaccctggc agcccgctgg aacggcacga cgtggacacg ggaacccaca cccaacggca
     2701 atcccgtcgg cttcaaccac ctcaacggcg tcgccgcggc gggcggcacg gtgtgggcgg
     2761 tcggcaccta tgtcgacccg aactcgtcga tcaaccggcg caagctgatc ctgcagcgcg
     2821 caggcggcag ctggcgcgtc tccgcggcac cgacagtggc gacgtacgag cacctcgcgg
     2881 ccgttgacgc caccgggctg gcggacgcct gggcggtcgg ctcggccacc agcaacatcc
     2941 agagcgcgcc gctggtaccg ctcgcgttgc gctggaacgg caccagctgg gcgtcgatga
     3001 cactgccagc caccgcaagc accgcgttga ccggagtcga cgcgcgcaca cccagcgacg
     3061 tgtgggtggc cggatcgtcg tccaacgccg acgggacaca accgtatgtc gcgcgcttcg
     3121 acggcacgtc ctggcgccgc atggccacac cgaccatccc cggcggcggc caactcagcg
     3181 acgtggtggc actgtcgccg tcggccgtcg tcgcggtcgg ccggtccaac ggcgccccgc
     3241 tcgtcctgcg ctgggacggc acgtcctgga cacgggaaag caccccagcg tcgtcgaacc
     3301 cctacctcac cggcgccgcc gccgctggcc ccaacgcggt ctgggcggtc gggtaccgct
     3361 tcgacctcaa cgcctactcc aaccggacgc tgaccatgct cgggacctga cggcaggcta
     3421 ccgggccggc gcccaacacg gcgtggccag cagagccggg gcagagcgcc gacttccagc
     3481 attcgcaacg accggatctg ccgcgacctg ttcgcggtcg gacgcgccca gggtggtcgc
     3541 cggtacgcct catcgattca gcgtcaaggc ggatcctgga cgattgtcca gccaccggtg
     3601 gagcaaccac gccgcggcga gtcccagccc gcctatcgcc actgccgcgg cggtcagcga
     3661 cgcggccgcg gtgagcgcgc cgatcagcag ggggccaccg ccgatgccca tatctgcgca
     3721 gaccctccag gcgccgagga actgggcgcg gcccaccgtg ggcgagacgt cgg
//
LOCUS       DASRJA010000002         6712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3103117,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000002 DASRJA010000000
VERSION     DASRJA010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3103117"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /gene="nuoE"
                     /locus_tag="VFC01_00025"
     CDS             complement(<1..736)
                     /gene="nuoE"
                     /locus_tag="VFC01_00025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HZN78065.1"
                     /translation="MSVFLELGQRPDEAGPPIHGPKTYPAEVVARLETDAEAIISRYP
                     QSRSALLPLLHLVQSQDGCLTPAGIAFCATQLGLTAAEVTAVATFYSMYRRTPTGEYL
                     VGVCTNTLCAIMGGDAILEALQDHLGVHAGETTDDGRVTLEHVECNAACDYAPVVMVN
                     WEFFDNQTPSSARDLVDGLRAGQPPTPSRGAPLCTFKETARVLAGLPDPEAAAAQGAV
                     GDATLAGLRVARERGMEAPDVQDATAA"
     gene            complement(733..2067)
                     /gene="nuoD"
                     /locus_tag="VFC01_00030"
     CDS             complement(733..2067)
                     /gene="nuoD"
                     /locus_tag="VFC01_00030"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HZN78066.1"
                     /translation="MSASPNPPESGGTERVVVVGGQDWDQVVAAARENAASHVGERIV
                     VNMGPQHPSTHGVLRLILEIEGETIVQIRAGIGYLHTGIEKNLEFRTWTQGVTFVTRM
                     DYLSPLFNETVYCLGVEKLLDVTDDIPERASVIRVMMMELNRISSHLVALATGGMELG
                     SMGAMFYGFREREQILSIFETITGLRMNNSYIRPGGVAMDLPDEAVPQIRDMLKLMPK
                     RLKDLEDLLSENYIWKARTQGIGYLDLTGCMALGITGPCLRSTGLPHDLRRAQPYCGY
                     ENYEFDVITDDGCDSYGRYLIRVKEMHESLKIVEQCLDKLKPGPVMILDKKLAWPADL
                     KLGPDGLGNSPEHIAKIMGHSMEGLIHHFKLVTEGIRVPAGQVYVAVESPRGELGVHM
                     VSDGGTRPYRVHYRDPSFNNLQAVAAMCEGGMVADAITAVASIDPVMGGVDR"
     gene            complement(2067..2771)
                     /locus_tag="VFC01_00035"
     CDS             complement(2067..2771)
                     /locus_tag="VFC01_00035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZN78067.1"
                     /translation="MAAVTENDNGHQADLNPPEVIGVRRGMFGATGSGDTSGYGRLVR
                     PVALPGSSPRPYGGYFDDVVDKLAAALGDDGYAASIERVVVYRDELTLEVQRQQLPAV
                     AQILRDDSGLRFELCLGVSGVHYPDDSGRELHAVYPLMSITNNRRIRVEVAAPDEDPH
                     IPSLFSVYPTVDWHERETYDFFGIIFDGHPALTRIEMPDDWVGHPQRKDYPLGGIPVE
                     YHGAEIPPPDQRRAYS"
     gene            complement(2759..3313)
                     /locus_tag="VFC01_00040"
     CDS             complement(2759..3313)
                     /locus_tag="VFC01_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HZN78068.1"
                     /translation="MGLEEKLPGGILLSTVEKVAGYIRKGSLWPATFGLACCAIEMMA
                     TAGPRFDISRFGMERFSATPRQADLMIVAGRVSQKMAPVLRQIYDQMAEPKWVLAMGV
                     CASSGGMFNNYAIVQGVDHVVPVDIYLPGCPPRPEMLLHAILKLHDKIQEMPLGVHRE
                     EAIREAEQAALAVTPTIELKGLLR"
     gene            complement(3304..3675)
                     /locus_tag="VFC01_00045"
     CDS             complement(3304..3675)
                     /locus_tag="VFC01_00045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZN78069.1"
                     /translation="MDLYTPILVLGAIAAAFAVGSVGIALLVGPRRYNHSKLEAYECG
                     IEPVPELASAHSTGQRFPIKYYLTAMLFIIFDIEIVFLYPWAVAFDSLGVFALVEMLL
                     FMVVVFVAYAYVWRRGGLTWD"
     gene            complement(3840..4241)
                     /locus_tag="VFC01_00050"
     CDS             complement(3840..4241)
                     /locus_tag="VFC01_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78070.1"
                     /translation="MTQPSRPLRILVYSDNPRTREQVQLALGKRVHPDLPELTYLEVA
                     TGPMVIRQMDEGGFDLVILDGEATPTGGMGIAKQLKDEIADCPPILVLTGRPDDAWLA
                     NWSRAEAAVPHPIDPIQLGDAVVSLLRAPVQ"
     gene            complement(4252..4782)
                     /locus_tag="VFC01_00055"
     CDS             complement(4252..4782)
                     /locus_tag="VFC01_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZN78071.1"
                     /translation="MTSAQKLLSNPASVGVWSVDPARSTIGFKSRSFWGLTPVKGRFA
                     EFSGDGQVTNTQTVFGRIDIKAASLDTKIRKRNEHLRSADYFEVAKFPDISVIVTGAD
                     AIDGDTVDLRAQLTIKSTTAPIALRTKVAVLDDGAVRLSTRATIDRKDFGVDGNLMGM
                     AIDKATILGDVVFRRG"
     gene            complement(4793..5182)
                     /locus_tag="VFC01_00060"
     CDS             complement(4793..5182)
                     /locus_tag="VFC01_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN78072.1"
                     /translation="MSVADAEAIADVADRLFGGIEQSDIALVEQLWDSDVIVWKVADR
                     DRDRERALRVIAWFINATTDRRYEILDRQFFDHGFVQQHILHANGRNGGSISMRVCIV
                     IKLGANGLISRIDEYFDPAEMAPLLGS"
     gene            5235..5864
                     /locus_tag="VFC01_00065"
     CDS             5235..5864
                     /locus_tag="VFC01_00065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN78073.1"
                     /translation="MDSTPTFSRFDDAYKTRTAPWVIDEPQPAIVELQRAGWIHSKVL
                     DIGCGTGEHTILLTRLGYDVLGIDFSPHAVEQARASAAEKGVDARFEVADAMNLGSDP
                     GYQTVVDSALFHIFDDADRARYVSSLHDAVRPGGLVHVLALSDAGRGFGPQVSGADIR
                     GAFGEGWVLEALDTTTYRGVVQEAQADAIGLPVGTRVDEPAWLARVRRV"
     gene            complement(5865..6485)
                     /locus_tag="VFC01_00070"
     CDS             complement(5865..6485)
                     /locus_tag="VFC01_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HZN78074.1"
                     /translation="MLTNQDIRSFHRAAVLASVDVVNGVRPEDLDRQTPCEGWNLSQL
                     LAHMTVQHRGFAAAARGDGADLARWDSATVADAVATDPAGAYAGAVTDVLAAFSDDDV
                     LAASFALPEFGPGAEFPGAQAIGFHFVDYVVHGWDVARSIDAPFTLPDDVISAVLPLV
                     FAVPDGDFRAAPGSPFARAIEAGDGVSDLGRVLLHLGRSPAWTLPR"
     gene            6537..>6712
                     /locus_tag="VFC01_00075"
     CDS             6537..>6712
                     /locus_tag="VFC01_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN78075.1"
                     /translation="MTPRPKRPDLAAMLAPLIREMVAAELPVLEAHGVSMWGYSVLVA
                     LDRSAIRTQAALAEA"
BASE COUNT         1184 a         2139 c         2185 g         1204 t
ORIGIN      
        1 acgccgccgt tgcgtcttgt acgtctggtg cttccatgcc gcgttcccgc gcgacgcgca
       61 acccggccag cgtggcgtct ccgacggctc cctgcgcggc ggcggcttcc gggtcaggca
      121 gtcccgcaag gactctcgcg gtctccttga aggtgcacag cggcgcgccg cggctcggcg
      181 tcggcggctg tccggcgcgc aaaccgtcga cgaggtcgcg cgccgacgac ggcgtctggt
      241 tgtcgaagaa ctcccagttg accattacca cgggggcgta atcgcatgcg gcgttgcact
      301 cgacgtgctc gagagtgacg cggccgtcgt cggtggtctc gccggcgtgt actcctaaat
      361 ggtcttgcag cgcttcgaga attgcgtcgc cgcccatgat cgcgcacagc gtgttggtgc
      421 agacaccgac gaggtattca cccgtcggcg tgcggcggta catcgagtag aacgtggcaa
      481 ccgcggtgac ttcggcggcc gtcaatccca gctgtgtggc gcagaacgcg atgcccgccg
      541 gggtgaggca gccgtcctgc gactgcacaa gatgcagcaa gggcagcagc gccgaacgtg
      601 attgcgggta acgggaaatg atcgcctcgg cgtcggtctc caatcgcgcg accacctcgg
      661 cgggataggt cttcggcccg tgaatcggcg ggcccgcctc gtcgggccgc tggccgagtt
      721 caagaaacac actcacctgt ccaccccacc catcaccgga tcgatcgagg ccacggcggt
      781 gatcgcgtcg gcgaccatgc cgccctcgca catggcggcc acggcctgca ggttgttgaa
      841 cgacggatcg cggtagtgca cccggtatgg tcgcgtgcca ccgtcggaga ccatgtgcac
      901 gccgagctca ccgcgcggcg actcgacggc gacgtacacc tggcctgccg ggacccggat
      961 gccctcggtg acgagcttga agtggtggat cagtccctcc atggagtggc ccatgatctt
     1021 ggcgatgtgt tcgggcgagt tgccgagacc gtccggcccg agcttcaggt cggcgggcca
     1081 cgccagtttc ttgtccagaa tcatcacggg gccaggcttt agtttgtcga ggcactgctc
     1141 gacgatcttc agcgactcgt gcatctcctt gacccggatc aggtagcggc cgtaggagtc
     1201 gcaaccgtcg tcggtgatga cgtcgaactc gtagttctcg tacccgcagt agggctgcgc
     1261 cctgcgcagg tcgtggggga ggccggtgga ccgcagacac gggccggtga tgcccaacgc
     1321 catgcacccg gtcagatcga gatagccgat gccctgggtg cgggccttcc agatgtagtt
     1381 ctcggacagc aggtcctcga gatccttcag ccgcttcggc atcagcttga gcatgtcgcg
     1441 gatctgcggg acggcttcgt cgggcaggtc catcgccacg ccgccgggcc ggatgtacga
     1501 attgttcatc cgcagcccgg tgatggtctc gaagatcgac aggatctgct cgcgctcgcg
     1561 gaacccgtag aacatcgcgc ccatcgagcc cagctccatg ccgcccgtcg cgagtgcgac
     1621 cagatgtgag gagatgcggt tgagctccat catcattacg cggatgacgc tggcccgctc
     1681 ggggatgtcg tcggtcacgt cgagcagctt ctcgacgccg aggcagtaca ccgtttcgtt
     1741 gaaaagcggt gagaggtaat ccattcgggt cacaaaggtg acgccctgtg tccaggtgcg
     1801 gaactcgagg ttcttctcga tgccggtgtg caggtaccca atgcccgccc ggatttggac
     1861 gatggtttcg ccctcgatct ccaggatgag gcgcagcacc ccgtgcgtgg agggatgctg
     1921 cggacccatg ttcacgacga tgcgttcgcc gacgtgtgaa gcggcgtttt cccttgcggc
     1981 cgcaacgact tggtcccagt cctggccgcc gactacgacc accctttcgg taccgcccga
     2041 ctcgggcggg ttgggggatg cgctcatcag ctgtaggccc tccgctgatc tggcggtggt
     2101 atctcggcgc cgtgatactc cacgggaatg ccgcccaacg ggtagtcctt gcgttgcggg
     2161 tgcccgaccc agtcgtcggg catctcgatg cgggtcaggg cgggatgacc gtcgaagatg
     2221 atgccgaaga agtcgtaggt ctcgcgctca tgccaatcga cggtcggata caccgagaac
     2281 agggacggaa tgtgtgggtc ctcgtctggt gccgccactt ccacgcggat gcggcgattg
     2341 ttcgtgatcg acatcagcgg gtaaacggcg tgcagctcgc gtccgctgtc gtcggggtag
     2401 tggacgccgc tgacgcccag acacagctcg aatcgcaggc cggagtcgtc gcgcagaatc
     2461 tgcgcgactg cgggcagttg ctggcgctga acctccagcg tcagttcgtc gcggtagacc
     2521 accacccgtt cgatcgatgc ggcgtagcca tcgtccccga gtgcggcggc gagcttgtcg
     2581 acgacgtcgt cgaaatatcc gccgtacggc cgcggtgagc tgccggggag ggcaaccggg
     2641 cggacgaggc ggccatagcc cgacgtgtct ccagagcccg ttgccccgaa catgccgcgg
     2701 cgtacgccga tgacctccgg aggattgaga tcagcctggt ggccgttgtc gttttccgtc
     2761 accgcagcaa tcccttgagc tcgatggtcg gcgtaaccgc caacgctgcc tgctcggctt
     2821 cgcggatcgc ctcctcacgg tgcacgccga gcggcatctc ctgaatcttg tcgtgcagct
     2881 tgaggattgc gtgcaacagc atttcgggtc gcggtgggca tccgggcaga tagatgtcaa
     2941 cgggcaccac gtggtcgacg ccttggacaa tcgcgtagtt attgaacatc ccgcccgacg
     3001 aggcacacac acccatcgcc agcacccact tgggctcggc catttggtcg tagatctggc
     3061 gaagcaccgg cgccatcttc tggctgacgc ggcctgcgac gatcatcagg tccgcctgtc
     3121 gaggcgttgc cgagaaccgt tccatcccga accgggagat atcgaagcgc ggccccgccg
     3181 tggccatcat ctcgatcgcg cagcaagcca ggccgaaggt cgcgggccac agggagccct
     3241 tccggatgta gccggccacc ttttcaaccg tggacagcag gattccgccg ggcagctttt
     3301 cctctagtcc catgtcaggc ctccccgacg ccagacatac gcataggcca cgaacaccac
     3361 gaccatgaag agaagcattt ctaccagggc aaacacgccg agactgtcga aggcgaccgc
     3421 ccacgggtag aggaacacaa tctcgatgtc gaagatgatg aacaacatcg cagtcaggta
     3481 gtacttaatc ggaaatcgct ggcctgtcga gtgcgccgac gccagttccg gcaccggttc
     3541 gatgccgcac tcgtaggcct ctaatttgga gtggttgtag cgccttggtc cgaccagcaa
     3601 agcaatgccg accgaaccga ctgcaaacgc agccgcaatc gcgccgagta ccaagattgg
     3661 cgtgtacaaa tccataccga gcaaacgctc cctcgtgggc tgtcgatgtg agcttaccca
     3721 caccctatcg agctttatga cgggtgccac gcattttgat tagagtcgct attagctgcc
     3781 tgcggccatg ttggcgtgcc tcgaaaaggc aagggcagca gcgcgattgg cgcagtggac
     3841 tactgcacgg gggcgcgcag cagagacacc acggcgtcgc ccaactggat cgggtcgatc
     3901 gggtgcggga cggcggcctc cgcgcgcgac cagttggcca gccaagcgtc gtccggccgg
     3961 ccggtgagca cgagtatcgg cgggcaatcg gcaatctcgt ccttgagctg ttttgcgatt
     4021 cccatgccgc cggtgggcgt ggcctcacca tcgagaatca ccagatcgaa gccgccctca
     4081 tccatctggc gaatcaccat cggaccggtg gccacctcga ggtaggtcag ctcggggagg
     4141 tcggggtgca cccgcttgcc caacgccagc tgcacctgct cgcgggtcct tgggttgtcg
     4201 ctgtagacga ggatgcgaag cgggcgagag ggctgggtca tgggccagat gctagccgcg
     4261 ccggaacacc acgtcaccga gaatcgtggc cttgtcgatg gccatgccca tcagattgcc
     4321 gtcgacgccg aagtccttgc ggtcgatggt ggcccgcgtg ctcagccgca ccgcgccgtc
     4381 gtcgagcact gcgaccttgg tgcgcagagc aatcggcgct gtcgtgctct tgatggtcag
     4441 ttgcgcacgc agatcgactg tgtcgccgtc gatagcgtcc gcaccggtga ctatcacgct
     4501 gatgtccgga aacttcgcca cctcgaagta gtccgcggag cgcaggtgct cgttgcgctt
     4561 gcgtatcttg gtgtccagcg acgctgcctt gatgtcgatg cgaccgaaaa ccgtttgggt
     4621 atttgtgact tggccgtcac cgctgaactc cgcgaagcgg cccttgaccg gcgtgaggcc
     4681 ccagaacgac ctgcttttga acccgatcgt cgaacgtgca ggatcgacgc tccagacgcc
     4741 gacggatgcc ggattgctga gaagcttctg tgcgcttgtc atgccacctc cgctatgacc
     4801 cgagcagcgg ggccatctct gccgggtcga agtattcgtc aatgcgactg atcaaaccgt
     4861 tggcgcccaa cttgatcacg atgcagaccc gcatcgagat cgatccgccg ttgcggccgt
     4921 tagcgtgcag gatgtgttgc tgcacaaaac cgtggtcgaa gaactggcgg tcgaggattt
     4981 cgtagcggcg atcggtcgtc gcattgatga accaggcgat gactcgcagc gcgcgctccc
     5041 tgtcgcggtc gcggtcagca accttccaga caatgacatc ggagtcccac aactgctcca
     5101 ccaacgcgat atcgctctgc tcgatcccgc cgaacaggcg gtcggccacg tcggcgatgg
     5161 cttccgcatc cgcgacggac atatctgtct tctcccttga cctcaagttg ggttgaggta
     5221 gaactctggt ctccatggat tcaacaccaa cattctcccg attcgacgat gcctacaaga
     5281 cccgaaccgc accgtgggtg atcgacgagc cgcagcccgc gatcgtcgag ctgcaacggg
     5341 ctggctggat tcacagcaag gtgctcgaca tcggttgtgg gactggcgaa cacacgatcc
     5401 tgctcactcg cttggggtac gacgtgctcg gcatcgactt ctcaccgcac gcggtcgaac
     5461 aggcgcgggc gagtgccgcg gagaagggcg tcgacgcgcg gttcgaggtg gccgacgcga
     5521 tgaatctcgg ctcagatccc ggctaccaaa cggtcgtcga cagcgcccta ttccatatct
     5581 tcgatgacgc cgaccgcgcc cggtatgtca gcagcctgca cgacgcggtc cggccaggcg
     5641 gcttggtgca tgtgcttgcg ctgtccgatg caggccgcgg gttcgggccg caggtcagcg
     5701 gggccgacat ccgcggggca ttcggcgagg gctgggtgct cgaagcactg gacaccacga
     5761 cctaccgcgg tgtcgtgcag gaggcgcagg ccgatgcgat cggcctgcca gtggggaccc
     5821 gcgtcgacga gcccgcctgg ctggcacgcg tcaggcgggt gtagtcagcg aggcagcgtc
     5881 caggccggcg atcggccgag gtgcagcagt acccggccga ggtcgctcac cccgtcgcca
     5941 gcctcgatgg cccgagcaaa cggcgagccc ggcgcggccc ggaagtcgcc gtcgggaacg
     6001 gcgaagacca gcggcaggac cgccgagata acgtcgtcag gcagcgtgaa cggcgcatcg
     6061 atcgaccgcg ccacatccca gccgtggacg acgtagtcga cgaagtggaa gccgatcgcc
     6121 tgtgcgccgg gaaactcggc gccggggccg aattcgggaa gagcgaacga tgccgcgagc
     6181 acgtcgtcgt cgctgaatgc cgcgaggacg tcggtcacgg ccccggcata tgcaccggcc
     6241 ggatcggtcg ccacggcgtc agccaccgtc gcagagtccc agcgggcgag gtcggcgccg
     6301 tcgccccggg ccgccgcggc gaatccgcga tgctgcacgg tcatatgcgc cagcaactgg
     6361 gagaggttcc aaccctcgca tggagtctga cgatcgagat cttcaggtct tacgccgttg
     6421 acgacgtcga cggaggccaa cacggcggca cgatgaaacg agcggatgtc ctgattagta
     6481 agcatacgta gatcatatgt atctgcttat catcggtcaa tgtttagtat cccggtgtga
     6541 ccccacggcc gaagcggccc gacctcgctg ccatgctcgc gccgcttatt cgggagatgg
     6601 tcgccgccga acttcctgtg ctggaagcgc acggggtgtc gatgtggggg tacagcgtgc
     6661 tggtcgccct cgatcgatcg gctatccgca cgcaggcggc actcgccgaa gc
//
LOCUS       DASRJA010000003         4754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1406983,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000003 DASRJA010000000
VERSION     DASRJA010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406983"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFC01_00080"
     CDS             <1..371
                     /locus_tag="VFC01_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HZN78076.1"
                     /translation="TAYFALKVERKSASEEDGVARPLLYQFWERFPKFVLGFIAASVI
                     GTLYLQWVPSGKAEIATVNDLRTWFLIFAFVSIGLEFSLRGLREAGWRPIAVFASATV
                     VNIVVALGLATVLFGNFQIG"
     gene            388..1344
                     /locus_tag="VFC01_00085"
     CDS             388..1344
                     /locus_tag="VFC01_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN78077.1"
                     /translation="MRAALSRRSFLTITAGAALAAACSSPKPGEVAKDGSVTVKHIFG
                     ETKIPAPPKRVVSAGFTEQDDLLAVGIVPIAVTDWFGGEPFGVWPWAQPKLGGAQPIV
                     LNLNDGIQVDQIASLKPDLIVATNAGLDQDTYSKLSAIAPTIAQSGPDAFFEPWKAQA
                     TAIGQAVFKNDDMAKLVAGVDEKFAGVGKTNPQFAGKKVLLVGGTFFEDSVRVTTPGW
                     RTDFLTNMGFTVPDAGAGLVPRDKIASVLDDADVLIWTTESEDEQTALLADPVVSKLR
                     ATTANRNVFTGKDLAGAIAFSSTLSYPVVADQLPPLIAKALA"
     gene            1357..2049
                     /locus_tag="VFC01_00090"
     CDS             1357..2049
                     /locus_tag="VFC01_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HZN78078.1"
                     /translation="MTSDETATKEQHAAFAQVGSKYYPIFVAVFTALVIISNVTATKG
                     VAFGPIITDGGFIVFPLTYVIGDVLSEVYGFKAARRAIFLGFAMNGLAAFTFWVTIYL
                     PAADFYPNQEHLENIVHAYTQLIVAGLAGFIVGQTINAWVVVFIKERTKEKHLWARLV
                     GSTFAGQLGDTVVFCSIAAGAIGISTFGDFVTYTALGWIYKTAVEVIMLPVTYRVIAY
                     IKRHEPTYGAAL"
     gene            2124..2789
                     /locus_tag="VFC01_00095"
     CDS             2124..2789
                     /locus_tag="VFC01_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HZN78079.1"
                     /translation="MSMTADLMDVSALGTVHDYGDQALLLEFDSTAEVLAWTDALRGA
                     ELLGVLDIVPASRTVLVKLAGPRYQAPTRQRLAKLRVTAEALTEASTPHEADIEIDVV
                     YDGADLEEVARLTGMTTDQVVAAHTGTLWQVGFGGFAPGFAYLIGGDERLNVPRQSEP
                     RTKVPAGSVALAGEFSGVYPRESPGGWQLIGRTNAELWDVDRDPPALLRPGMWVQFRE
                     VRS"
     gene            2786..3670
                     /locus_tag="VFC01_00100"
     CDS             2786..3670
                     /locus_tag="VFC01_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HZN78080.1"
                     /translation="MTTTIEVLNTGPLALIEDLGRPGLAHMGVGRSGAADRRSHALAN
                     RLVANPGDHATIEVTFGGFSARVRGGDVAIAVTGADTDPSVNGKPFGTNSIHYARDGE
                     VITMSAPHSGLRTYLAVRGGIDVSPVLGSRSYDVMSAIGPLPLQPGDVLPVGEHSGEL
                     PELDQAPVAAIEDEVLELRVVPGPRDDWFVDPDILIRTNWQVTNRTDRVGMRLIGMPL
                     EYRWPDRQLPSEGATRGAIQVPPNGFPVILGPDHPVTGGYPVIGVVTDEDIDKVAQIR
                     PGQTVRLHWSRPRRPFED"
     gene            3708..4271
                     /locus_tag="VFC01_00105"
     CDS             3708..4271
                     /locus_tag="VFC01_00105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN78081.1"
                     /translation="MHTQEPRVRGIHTARLVHTADLDNETREDARRMVIEAFAGEFSD
                     ADWEHSLGGMHALICNHGTLVAHAAVVQRRLLYGGTALRCGYVEGVAVREDWRGQGLA
                     GALMDAIEQVLRGAYQLGALSASEAGRHMYTARGWLPWRGPTSVLAPAGLSRTPDDDN
                     GLFVLPVTVELDTTAEITCDWRDGDVW"
     gene            4291..>4754
                     /locus_tag="VFC01_00110"
     CDS             4291..>4754
                     /locus_tag="VFC01_00110"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN78082.1"
                     /translation="MTTPASFRPTPEAFDKLYRGEATFEGAPPPTAIPWDVHQAQPRL
                     MEVEALGGISGEVLDIGCGLGDNSIFLASRGHSVTGLDGSPAAIEEARRRAADAGVTV
                     TFDVADATKLTRYDGRFDTVVDSALYHCLDEEGQQAYIAALYRVTRPGARLHL"
BASE COUNT          807 a         1564 c         1571 g          812 t
ORIGIN      
        1 tgaccgccta cttcgcgctg aaggtcgaac gcaagtccgc cagtgaagag gacggggtgg
       61 cgcgtcctct tctttaccaa ttctgggaac gtttcccgaa gttcgtgctc ggcttcatcg
      121 ccgcatcggt gatcggcacg ctgtatctgc agtgggtgcc cagcggtaag gcggagatcg
      181 ccaccgtgaa cgacctgcgc acgtggttcc tgatcttcgc gttcgtgtcg atcggcttgg
      241 agttttcgct gagggggctg agagaagcgg gctggaggcc gattgcggtg ttcgcctcgg
      301 ccacggtcgt gaacatcgtc gtcgcgcttg gcctcgccac cgtgttgttc ggcaacttcc
      361 agattggtta gcggataggc tcgccgagtg cgagctgcgt tgtcccgccg aagtttcctc
      421 acaatcactg ctggcgcggc gctggcggcg gcatgcagtt caccgaagcc cggcgaagtc
      481 gccaaggatg gctcggtcac cgtcaagcac atcttcggtg agaccaagat ccccgcaccg
      541 cccaagcgcg tcgtcagcgc cggcttcacc gaacaggacg acctgctcgc ggtcggaatc
      601 gtcccgatcg cggtcaccga ttggttcggc ggtgagccgt tcggcgtatg gccttgggcg
      661 cagccaaaac tcggcggcgc acagcccatc gtgctcaacc tcaacgacgg catccaggtg
      721 gatcagatcg cgtcgctgaa gcccgacctg atcgtcgcca ccaacgccgg actggaccag
      781 gacacctaca gcaagttgtc ggccatcgcg ccgacgatcg cgcagtccgg gcccgacgca
      841 ttcttcgagc catggaaggc ccaggccacc gctatcgggc aggccgtgtt caagaacgac
      901 gacatggcca agctcgtcgc gggcgtcgac gagaagttcg caggcgtcgg caagaccaac
      961 ccccagttcg ccggcaagaa ggtgctacta gtcggcggca ccttctttga agacagcgtt
     1021 cgcgtcacga cgccgggctg gcgcaccgac ttcctgacca acatgggctt caccgttccc
     1081 gacgccggcg ccggcttggt gccgcgcgac aagattgcgt ccgtccttga cgacgccgac
     1141 gtgctgatct ggacgaccga gagcgaagac gaacagaccg cgctgcttgc ggatcccgtc
     1201 gtctcaaagc tgcgggcgac cactgcgaac cgcaatgtct tcaccggcaa ggatctcgcc
     1261 ggtgcgatcg cgttctcctc gacactgtcc taccccgtgg tggccgacca gctgccgccg
     1321 ctcatcgcga aggcgctcgc ctgatctaat gtgcacgtga ccagcgacga gaccgccacc
     1381 aaagaacagc acgccgcgtt cgcgcaggtg ggatcgaagt actatccgat cttcgttgcg
     1441 gtcttcaccg cgctggtgat catttcgaac gtcaccgcga cgaagggcgt cgcgttcggc
     1501 ccgatcatca ccgacggcgg gttcatcgtc ttcccgctca cctacgtgat cggcgacgtg
     1561 ctgtccgagg tgtacggatt caaggccgcg cgcagggcga tcttcctcgg gtttgctatg
     1621 aacggcctgg ccgcgttcac gttctgggtg acgatctatc tgcccgcggc cgacttctac
     1681 cccaaccagg agcacttaga gaacatcgtc cacgcctaca cccaactgat cgtggcgggg
     1741 ctggccggct tcatcgtcgg gcagacgatc aacgcgtggg tagtggtgtt catcaaggag
     1801 cgcaccaagg agaagcacct ctgggcgcgg ctggtcggct cgactttcgc gggccaactg
     1861 ggtgacacgg tggtgttctg cagcatcgca gcgggcgcca tcggcatcag caccttcggc
     1921 gacttcgtca cctacaccgc attgggttgg atctacaaga ccgccgtcga ggtgatcatg
     1981 ctgcccgtca cctatcgcgt gatcgcttac atcaagcgcc acgagccgac ttacggcgcc
     2041 gctctatgag ctatcgctaa ggcctctctg gcaaggctca tagagctaga gctttcttag
     2101 ctactcgcgg gtaagttcgg atcatgagca tgactgcgga tctgatggac gtaagtgccc
     2161 tcggcacggt ccatgactat ggcgatcagg cgctattgct tgagttcgac agcacagcag
     2221 aagtattggc gtggaccgac gcacttcgcg gggccgagct gctcggcgtg ctggacatcg
     2281 tgcccgcgtc gcgcacggtg ctggtcaagc tcgccggacc gcgctatcag gcgccgaccc
     2341 ggcaacggct ggccaagctc cgcgtgacgg cagaggcgct gacggaggcg agcacacctc
     2401 acgaggccga catcgagatc gatgtggtgt acgacggcgc cgacctggaa gaggtggccc
     2461 ggctcaccgg catgaccacc gaccaagtgg tggccgcgca caccggcacg ctgtggcagg
     2521 tcggattcgg cggtttcgca ccgggtttcg catatctcat cggtggcgat gaacggctca
     2581 acgtgccgcg gcagtccgag ccgcgaacca aggtgcccgc aggttcggtg gcgctggccg
     2641 gggagttcag cggcgtctac ccgcgggaat cgcccggggg ttggcaactc atcgggcgca
     2701 cgaatgcgga gctgtgggat gtcgaccgcg acccacctgc cctgctcaga cccggcatgt
     2761 gggtgcagtt ccgagaggtg cggtcatgac caccacaatc gaggtcctca acaccggacc
     2821 gctcgccctg atcgaagatc tcggccggcc ggggctggcg cacatgggtg tcggccgctc
     2881 gggcgccgcc gatcgccgtt cgcacgcgct ggccaaccgg ctggtcgcca accccggcga
     2941 ccacgccacc atcgaggtga cgttcggcgg gttttcggcg cgggtgcggg gcggcgacgt
     3001 cgctatcgcg gtgacgggag ccgacaccga tccctctgta aacggaaaac cgtttggcac
     3061 caacagcatt cactatgcgc gcgacggcga agtgatcacg atgagtgcgc cgcattccgg
     3121 cctgcgcacg tacctggcgg tgcggggcgg catcgacgtt tcgcccgtgc tgggttcgcg
     3181 ctcctatgac gtgatgtcgg cgatcggtcc gctgccgctt cagcccggcg atgtcctccc
     3241 ggtcggcgaa cactccggcg aactccccga gctggaccag gcgccggtcg ccgccatcga
     3301 ggatgaggtg ctggagctga gggtggtccc cggcccgcgc gacgactggt tcgtcgatcc
     3361 ggacatcttg atccgcacca attggcaggt caccaaccgc acggaccggg tggggatgcg
     3421 gctgatcggg atgccgctgg agtaccgctg gccggaccgt cagctgccca gcgaaggcgc
     3481 gacccgcggc gcaatccagg tgccgccgaa cggattcccg gtgatcctcg gccccgatca
     3541 cccggtgacc ggcggctatc cggtgatcgg cgtggtgacc gacgaggaca tcgacaaggt
     3601 cgcccagatc cgacccgggc agacggtgcg gctgcactgg tcgcgtccgc ggagaccgtt
     3661 cgaggactag ccgagacacc ggcgcctcgc gctggtagaa cggcagtgtg cacacgcagg
     3721 aacctcgcgt gcgcgggatc cacaccgccc gcctggtcca caccgccgac ctcgacaacg
     3781 agacccgcga agacgcgcgc cggatggtca tcgaggcctt cgccggtgaa ttctccgacg
     3841 ccgattggga gcactcgctc ggcggcatgc acgccctgat ctgtaaccac ggcacgctcg
     3901 ttgcgcacgc cgccgttgtg cagcgccggc tgctgtacgg cgggactgcg ctgcgttgcg
     3961 gctacgtcga aggtgtggcg gtgcgcgaag attggcgcgg gcagggcctc gccggagcgc
     4021 tgatggacgc catcgagcag gtgctgcgcg gcgcatacca actcggtgcg ctgagcgcgt
     4081 cggaggcggg taggcacatg tacacggcgc gcgggtggct gccatggcgc gggccgacat
     4141 cggtgctggc gccggccggt ctcagtcgca cgcccgacga cgacaacggg ctgttcgtgc
     4201 tcccggtcac ggtcgaactc gacaccaccg ccgagatcac ctgcgattgg cgcgacggcg
     4261 acgtctggta gcgcgcgtac gttggagaag gtgaccactc cagcgtcctt tcggcccacg
     4321 cccgaagcct tcgacaagct ctaccgcggg gaagccacgt tcgaaggcgc ccctccgccc
     4381 accgcgattc cgtgggacgt gcatcaagcg cagccgcgtt tgatggaagt cgaagcgctg
     4441 ggcggtattt cgggtgaagt gctggatatc ggctgcgggc tcggagacaa ctcgatattc
     4501 ctcgcctcgc gcggacattc ggtgaccggg ctggacggct cacccgccgc gatcgaagag
     4561 gctcgccgcc gcgcggccga cgccggtgtc acggtgacat tcgacgtcgc cgacgcgaca
     4621 aagctgacca ggtacgacgg tcggttcgac accgtcgtcg acagcgcgct gtatcactgt
     4681 ctggacgaag aagggcagca ggcctacatc gccgccctct accgcgtgac ccggccgggc
     4741 gcccgtctgc acct
//
LOCUS       DASRJA010000004        17829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2806568,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000004 DASRJA010000000
VERSION     DASRJA010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17829
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2806568"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VFC01_00115"
     CDS             complement(<1..846)
                     /locus_tag="VFC01_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZN78083.1"
                     /translation="MTVHTDVTTDELLGLFPPGTDRDSDGTLTVGGCRLDEVAEQFGT
                     PAIVVSEDALRQRARDYLAAFRGRWPRCDVAFASKSFPCTAVQRVMVEEGLHLDVAGG
                     GEILTALKAGADAAKLVLHGNAKSDEELELAVKHGVGLVVVDNFDDIDRLERIVPAGH
                     QQPCLVRVIPGVEAATHASQATGHAGSKFGLLPDDARDAIGRIGRSAKLRLDGVHTHV
                     GSQLLNVEQLAQAVEPIAKLGTFDVYDLGGGLGVRYTYDEHPPSLDDYADAMVTQAKA
                     LLPDSA"
     gene            945..1562
                     /locus_tag="VFC01_00120"
     CDS             945..1562
                     /locus_tag="VFC01_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN78084.1"
                     /translation="MIPTDVRPTKKQQQGEQSRELILDATERLMATRGYAATSINDIR
                     KACGLPPSSIYWHFGSKEGVLAAVMERGADRFFAAIPTSEDVGGQLAVLSTLQAQHPD
                     FLRLFYMLSLERGTDPAVAAVIRRVRDTAIRRFRDAIGALLPTGVPQDKTERVVTELA
                     SVAVALSDGVFFAEHLEPDGTDVARMYRRLYQTMTALIPILLEEK"
     gene            1559..2455
                     /locus_tag="VFC01_00125"
     CDS             1559..2455
                     /locus_tag="VFC01_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN78085.1"
                     /translation="MNTKTAIITGASSGLGLECARALLRSDPSWHVVLAVRDTGRGAE
                     AVGQLGEPQRCTVMRMDLASPASVRVFTETFRGSGLPPLRAIVCNAGVQVVSGTALTE
                     DGVEMTFGVNHLGHFALVQGLLGELAHPARILVVSSGTHDPSRHTGMPDPLYTSAADL
                     AHPRAEQSADGRRRYTTSKLCNVLFGYELNRRLGDGVTVNAFDPGLMPGSGLARDYPP
                     LQRLVWRYLLPALRVLPGVRSTRTSGRYLATLVNDARFDGVSGEYFDGLRPIRSSADS
                     YDRGKALDLWETSERLLAHAIK"
     gene            2484..3656
                     /locus_tag="VFC01_00130"
     CDS             2484..3656
                     /locus_tag="VFC01_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN78086.1"
                     /translation="MAQDRRARVAVAALFLTNGALFANLLPRYPEIKSDLHLSNAIYG
                     AAIAAFSGGALVAGLAAAALIRRFHSSRVAVAGTIGIAVFVIIAALATSPLALAAALF
                     VAGASDAITDVAQNAHGLRVQRNYGRSIINSFHAVWAVGAILGGLMGAGAIAIHMPRA
                     THLSIAGAVFSAVVVIAYPYLLRGPDHDDHPSARSAGRGGAGIAVYATLVALVVIAIA
                     GATVEDAGSSWATLYLRDSLGAPGAVAVFGYVALVGFMFVGRMTGDRLVDRFGERAVV
                     RAGGFVAAAGMGAALAFPSVPGTIAGFAAAGLGVATLIPAAMHGADQLPGMRPGTGLT
                     AVTWLMRIGFFGAPLLVGVVADATSLRVGLLSVPVSGVVVIALAGALSARRQLSRS"
     gene            complement(3625..3948)
                     /locus_tag="VFC01_00135"
     CDS             complement(3625..3948)
                     /locus_tag="VFC01_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HZN78087.1"
                     /translation="MWLSLAGAILVEVFATLALRASDGFRKKVWIAPVVAGYLLCFYL
                     LWVALSLGMPVGIAYGVWTACGVALVAVIARFLFAEPLTWVMVLGIAFIVAGVLTIEI
                     AGAVH"
     gene            complement(3948..4286)
                     /locus_tag="VFC01_00140"
     CDS             complement(3948..4286)
                     /locus_tag="VFC01_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HZN78088.1"
                     /translation="MRKWVLLIAAIAVEVAATLSLRASQDHSAWLIVVVAGYVGSFIL
                     LTMVLRTGMAVGVAYGIWGACGTAATAVLAAAIYGDPFTWPIVAGIGLIIAGVLLVEF
                     GSQRAAADES"
     gene            complement(4296..4931)
                     /locus_tag="VFC01_00145"
     CDS             complement(4296..4931)
                     /locus_tag="VFC01_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78089.1"
                     /translation="MIDGQLIEIVGGATAAGRVDESTDRTSQRILDAAVGEAAAVGLQ
                     RITVEDVVRRAGVSRMTVYRRYPRRDDLVEALVRRETQRFLAAVADAIDTVADPHEGV
                     AEAFIAAVRFATQHPMLRRAGQFESAPAADSVELLKLGSAFIANYIHGEAPGTPTQQV
                     RWVADMFARLFLTYISMPPTDPNFGDEAELRRFAHEVLTPMVERASQNGVA"
     gene            complement(4928..5833)
                     /locus_tag="VFC01_00150"
     CDS             complement(4928..5833)
                     /locus_tag="VFC01_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZN78090.1"
                     /translation="MDLRSRIVAEFEKGAGRHDDPAVYGGRPGDPGLIGPDSVSWEIN
                     ADLAAVSQAGLPAIVLEILHPSVVAGVQDLSNYRQDPFQRARATLGYVLTTTFGNTEA
                     ATGLIERVKGVHSFVTGTRPDGVPYRALDPELIAWVHTCIPWMIMRAYEHTKRPLSQQ
                     ERDRYLAEQAVIGRLGGAEWVPTTAAELDDYVEAMRPRLSVNAQTREFIDFLMTSPFF
                     PNLPGPVDRQLHRFAIYAGMSRAPQWARELIGFDRPSALTRVLIDPALQSDARRLRWA
                     FGTPRYVQLARQRAAGAELTTAAAP"
     gene            5945..7483
                     /locus_tag="VFC01_00155"
     CDS             5945..7483
                     /locus_tag="VFC01_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HZN78091.1"
                     /translation="MSTVVETAYGPIRGVDDGGVRSWLGVRYGAPPTGGLRWRAPEQP
                     QPWTDVADASILGPVCPQPVDPRIPIDLGAPQGDDCLTLNIWASSDIAAGDRKPVMVW
                     IHGGAYVLGSANQPMYDGRVLAAGGDVVVVTVNYRLGAFGFLDLSSFSTPRRRFESNL
                     GLRDVLHALHWIRDNIAAFGGDPDRVTVFGESAGAGIITTLLASPAAEGLFAGAIAQS
                     SPATSLYDATRGRRVAAQFLAALGLGDNDIEQLTDAPVEALVAASRTLFNEVPVQCPG
                     TLAFAPIVDGDLVPDHPVKLAREGRSHPVPLIIGTNKHEAALFRWMKSPLMPITPEAI
                     NAMFTQIAAEQPNLELPTDDQIDTAYPGLRGKARGMGVARDVGFRMPTVWLAEGHSTV
                     APVYLYRFDWATPMLRLLRLSGAHATELPFVWGNLVAGPKDPTFRLGGMKTGKVVSAR
                     IQARWRNFATQGKPLGLDGEPEWLPYQAADRACLVIDRQDTVLNDVDLHIRSAWGSEV
                     LSFR"
     gene            7555..8439
                     /locus_tag="VFC01_00160"
     CDS             7555..8439
                     /locus_tag="VFC01_00160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZN78092.1"
                     /translation="MHDPVMYAVPFFLLFLIIEWTAARKLAHVEAERPPSGAYLKPDA
                     WASIWMGLVSVGTSGVLNFIALLGYAALYAYVAPWHLPATQWYTWVIAIVGVDFLYYW
                     YHRMAHRVRIFWATHQAHHSSQYFNFATALRQKWNISGDVFLRALLPLFGVPPWLVFL
                     SFSINLIYQFWIHTERIDRLWRPIEFVFNTPSHHRVHHGMDQQYLDRNYGGIFILFDR
                     LFGSFQAETTRPHYGLTKQVDTFNIWTLQTFEYVAIARDVRHARRWRDRLGYIFGPPG
                     WVPAEDRVNTGGRPAPVP"
     gene            8450..9856
                     /locus_tag="VFC01_00165"
     CDS             8450..9856
                     /locus_tag="VFC01_00165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN78093.1"
                     /translation="METVFDPDVDPGLVERALAASTFGSMWLDIPRPEYPKATGSVIC
                     DLLVVGGGYTGLWTALHAAQRNPDRRIVLIEANRIGWAASGRNGGFVDASLTHGRENG
                     KARWPNEIDTLEAMGRDNLDGMQADIESLGLDAEWQRSGMLSVATEPHQVDWLADAAA
                     HGEGQLLGLREVRDEVHSPTYLAGLYNPATCAIVHPAKLAFELARACREAGVQIYEHT
                     NAQGLDSAGVALHIQAGEAAFTCRNAVLATNVFPSLLRRNRWHTVPVYDYVLATEPLT
                     DAQLDRIGWRRRQGIGDSANQFHYYRLTMDNRIVWGGYDAIYHFGRRVDDRYEDRAAT
                     YRRLAAHFFITFPSLDDVRFTHRWAGAIDTNTRFCAHWGLAREGRVAYVNGFTGLGVA
                     AARFAADVCLDLLDGEPTARTELEMARKRPLPFPPEPLASVGIQATRWSLDRADHSAG
                     RRNILLRALDALGLGFDS"
     gene            9979..10608
                     /locus_tag="VFC01_00170"
     CDS             9979..10608
                     /locus_tag="VFC01_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZN78094.1"
                     /translation="MSDRLMNGEKLERGQSLTSRNGAYTLTLQDDGNLVLAARGDAVW
                     ATGTNGQDVERLEVQKDGNCVLYTSDKPVWHTDTKGAKDVRLLLQDDRNLVLYGFDGP
                     AWASNTQTGEAPPAPPAPPAAEEPAGEPVAVAAEEPAPPAPEPAAAAAPPPPPPPPAP
                     RTYTVVSGDTLWAISERFYGDGSKYQVIADASGIANPDLIYPGQVLTIP"
     gene            complement(10619..10882)
                     /locus_tag="VFC01_00175"
     CDS             complement(10619..10882)
                     /locus_tag="VFC01_00175"
                     /inference="COORDINATES: protein motif:HMM:NF023159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZN78095.1"
                     /translation="MSESDLRALPNIGPAIARMLLRLGIERPDDLRGQTAEHLYRRLC
                     AMDGRRHDRCLLDTFVAAVDHANGAPARPWWYYSRLRKANGSN"
     gene            complement(10921..11775)
                     /locus_tag="VFC01_00180"
     CDS             complement(10921..11775)
                     /locus_tag="VFC01_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN78096.1"
                     /translation="MRVGIGLPAAVPGVDATVIGSWAAQSELVGFASLGVIDRLVYDN
                     LDPLVALAAAAARTERVEMLTTVLNVGYRRNPIVLAKQIASVDQLSGGRLTVGLALGG
                     WPEDYSASNASLSARGATFDGTLTAMRQVWSGDISGASGPMPALPTGRPSVLVGGLVP
                     ASFARMAAHAEGWVAPSFGMPVLKDGITSARRAWSNAGRPGQPRIVVERYFSLGPDAD
                     EIADHYLMHYYGSDYFPDVRADALTGRERLHDELQRLAEVGCDDVILLPCSGELDQVA
                     LLTDAVSD"
     gene            11974..12654
                     /locus_tag="VFC01_00185"
     CDS             11974..12654
                     /locus_tag="VFC01_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78097.1"
                     /translation="MRRLWLRMLAAAAAPIVALSLPSAPAQATAGVPVFPGMEVRQGT
                     NVCTLGYVDPQMRIAFTAGHCRGSGQVTDRDGNAIGSQAVFRDNTPNGATIDTNHQIA
                     DWEAISLAPDVAVNNILPVGRQLIADPAVVPVVGLPVCHFGVITGESCGTIEAVNNGW
                     FTMANGVVSQKGDSGGPVYNLTPDGRAAIVGMFNSTWGQYPAAVSWQAASEQAREDVI
                     SAASAPAP"
     gene            complement(12651..13091)
                     /locus_tag="VFC01_00190"
     CDS             complement(12651..13091)
                     /locus_tag="VFC01_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HZN78098.1"
                     /translation="MTVRYDAPNLAARAGNELIRWLAEAGISLAGTRALRVRGRKTGK
                     LRGVVINLLTVDGRDYVVSPRGNTQWARNARTAGSVEMGPRWRTRHVRISEIADDAKP
                     QLLKRYLERWYWEVKGHVGGLTPQSTDDEIRAVAPSIPVFELLR"
     gene            13194..13874
                     /locus_tag="VFC01_00195"
     CDS             13194..13874
                     /locus_tag="VFC01_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78099.1"
                     /translation="MGKRQETREKIEAQIVELGRRHLVTDSAAGLSLRAIARDLGMVS
                     SAVYRYVASRDDLLTLLLVDAYSELADAVDRAAATAGGSWRDRVLATAHAARAWAIDQ
                     PARWSLLYGSPVPGYHAPRERTVGPGTRVVASLFDAIGAGIAAGDIPQSNVAVAQPLS
                     SDFGRLREEFAFAGDDSAVAKCFLLWAGLVGAISLEVFGQYGADTLTEPGAVFDLQVR
                     LLINTLSI"
     gene            13919..14257
                     /locus_tag="VFC01_00200"
     CDS             13919..14257
                     /locus_tag="VFC01_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN78100.1"
                     /translation="MHAFDVLGDPVRRRILELLVDGEKSAGAIGVTIQGEFAITQPAV
                     SQHLKVLRDNGFTSVRPDGQRRLYAVSGRALRDVDEWLDGFRRFWTPHLDALHTEIAR
                     GKRQRRRGAT"
     gene            14254..14895
                     /locus_tag="VFC01_00205"
     CDS             14254..14895
                     /locus_tag="VFC01_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN78101.1"
                     /translation="MIEIDVDHQINAVQRKLGHRTIDAGEAHVLTVSQSYDTDQDDLW
                     DAVTNIERIPRWFLPISGDLQLGGSYQLEGQAGGKILTCDPPKNFTATWEFGGNVSWI
                     DVTITGDGPDRARLVLEHIAQVDDDEIWRQFGPGAVGMGWDSMLLGLALHLATGESID
                     PSFGQQWTGTDDGRRFLTLSGDEWYAVNVAFGTDPAAARAMADRCLKAYLGEE"
     gene            14946..15422
                     /locus_tag="VFC01_00210"
     CDS             14946..15422
                     /locus_tag="VFC01_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN78102.1"
                     /translation="MPDQTPVQIAWVTRDLDATEKALTTLLGAKKWIRLPGVHFGPDA
                     CTYRGRPADFVADISLSYAGDTQLELIAPVAGESIYTEFLNRAGAGLHHICIEADDVE
                     RAVAERDADVVQRGVMPGGMEFAYISADDAGVPYIEIAYISPEIRAFFDYIKQEQQ"
     gene            15454..16899
                     /locus_tag="VFC01_00215"
     CDS             15454..16899
                     /locus_tag="VFC01_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZN78103.1"
                     /translation="MTQIPETVNAAGVTSWSDEVDVLVIGFGIAGGCAAVSAAAAGAR
                     VLVLERAAAAGGTTSMAGGHFYLGGGTAVQQATGHDDSPDEMYKYLVAVSREPEHDKI
                     RAYCDGSVEHFNWLEDLGFQFERSYFPGKAVIQPNTEGLMFTGNEKVWPFKDQAVPAP
                     RGHKVPVPGDTGGAGMVIDLLLKRADELGVQIRYETGATALVLDNGAVVGATWKRFND
                     TGAIRAKAVVIAAGGFVMNPEMVAEFTPKLAEKPFVLGNTYDDGLGIRLGESAGGATK
                     HMDQVFITAPAYPPSLLLTGIIVNKLGQRFVAEDSYHSRTSGFVMDQPDSAAFLIVDE
                     EHLERPEVPLVPLIDGWEAVEEMEAALGIPEGNLVATLNRYNEYAARGEDPDFHKQPE
                     FLAPQDTGPWGAFDLSLGKAIYAGFTVGGLATSVDGQVLHEDGSVIPGLYAAGACASN
                     IAQDGKGYASGTQLGEGSFFGRRAGAHAARA"
     gene            complement(16896..>17829)
                     /locus_tag="VFC01_00220"
     CDS             complement(16896..>17829)
                     /locus_tag="VFC01_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN78104.1"
                     /translation="IANYRAFVRRTLMFLGWNRSISRIVDPLPTVVQAPRMFAGEIDF
                     GDVTQSSSAFRQLHDSLSWFRAVYDSFANYRAAILRLDGLVTSNEKARDLPTLTALPS
                     GDGSLELDDVEVRTPDGTRLIDPLDVRLDSGESLVITGGSGCGKTTLLRSLAQLWPFT
                     SGTVRGPADGTMFLSQLPYMPLGDLRTVISYPGAAGQFDDAAIQSALDTVALGHLAGR
                     LDEVADWAKVLSPGEQQRIAFARVLLAKPKVVFLDESTSALDEGQEFALYRSLRTALP
                     DCIVVSVSHRPTVEQHHDRRLELLGGGQWRLAPV"
BASE COUNT         2994 a         5847 c         6029 g         2959 t
ORIGIN      
        1 cgcgctgtcc ggcagcagcg ccttggcttg cgtgaccatc gcgtcggcgt agtcgtcgag
       61 gctcggcggg tgctcgtcgt aggtgtagcg caccccgagc ccgccaccga ggtcgtagac
      121 gtcgaaggtg ccgagcttcg cgatgggttc cacggcctgc gcgagctgtt caacgttgag
      181 cagttgcgat ccgacatggg tgtgcacccc gtcgagccgc aacttggcac tgcgcccgat
      241 cctgccgatg gcgtcgcggg cgtcgtcggg cagcaggccg aacttcgagc cggcgtgacc
      301 ggtcgcttgg gatgcgtggg tggccgcctc gacgccgggg atcacccgca cgaggcacgg
      361 ttgctgatga cccgccggga cgatgcgctc gagccggtcg atgtcgtcga aattgtcgac
      421 caccaccagg ccgacgccgt gcttgaccgc gagctcaagt tcctcgtcac tcttggcgtt
      481 gccgtgcaga acgagcttgg cggcatcggc gcccgccttg agcgcggtca ggatctcgcc
      541 gccgccggcg acgtcgaggt gtagcccctc ttcgaccatc acccgctgaa cggccgtgca
      601 cgggaacgac tttgacgcga acgccacatc gcagcgcggc catcggccgc ggaaagccgc
      661 gagatagtcg cgggcccgct gccgcagagc atcttcagac accacgatgg ccggggtgcc
      721 gaactgctcg gctacctcgt cgaggcggca cccaccgacc gtcagggtgc catcgctgtc
      781 gcggtctgtt cctgggggga agaggccgag caactcatcg gtcgtcacgt cggtatggac
      841 cgtcatgcgc tcagcgtgcc actgggacgc cgagcgtgaa ccggctgcga gaaaagtccg
      901 cgaaatccgc agtggcttca cattcgcctg ttactctgtc tgtaatgatc cctacagatg
      961 tacggccgac gaagaagcag cagcagggcg agcagtcccg tgagctgatc ctcgacgcga
     1021 ccgaacggct gatggccacc cgcggatacg ccgccacatc gatcaacgac atccgcaagg
     1081 cgtgcgggct gccgcccagc tccatctact ggcatttcgg ttccaaggaa ggcgtgctcg
     1141 cggcggtgat ggaacgcggc gccgaccggt tcttcgcagc gatcccgaca tcggaagacg
     1201 tcggaggaca gctggcggtg ctgtcgaccc tgcaggcgca acacccggat tttctgcggc
     1261 tgttctacat gctgtcgctg gaacgcggca ccgatccggc ggtcgccgcg gtgatccgcc
     1321 gcgtgcgcga caccgcgatc agaaggttcc gcgacgcgat cggcgcactg ctgcctaccg
     1381 gcgtgccgca ggacaagacc gaacgcgtcg tcaccgaact cgcctcagtg gctgtcgcgc
     1441 tgtcggacgg ggtgttcttc gccgagcacc tcgaacccga cggcaccgac gtcgcgcgga
     1501 tgtaccgacg gctgtatcag accatgacgg cgctcatccc gattctgttg gaggagaaat
     1561 gaacacgaag accgcgatca tcaccggcgc cagttcgggg ctcgggctcg aatgcgcacg
     1621 cgcactgttg cgcagcgacc cctcctggca tgtcgtcctt gcggtccgcg acaccggccg
     1681 cggcgccgag gcggtcgggc agctgggcga accgcagcgc tgcacagtga tgcggatgga
     1741 cctcgcttcg ccggcgtcgg tgcgcgtgtt caccgaaacc ttccgaggtt ccgggcttcc
     1801 gccgttgcgt gcgatcgtgt gcaacgccgg ggtccaggtg gtgtcgggca cggcgttgac
     1861 ggaggacggc gtcgagatga cgttcggtgt caatcatctc ggccacttcg cgctggtgca
     1921 gggccttctc ggcgagctgg cgcacccggc gagaatcctt gtcgtcagca gcggcaccca
     1981 cgatccgagc aggcacacgg ggatgcccga cccgctctac acctcggctg ccgacctcgc
     2041 gcatccgcgc gccgaacagt ccgccgacgg ccgtcgtcgc tacaccactt caaagctgtg
     2101 caacgtcctg ttcggctacg aactcaaccg caggctcggc gatggcgtaa ccgtcaacgc
     2161 attcgaccct gggctgatgc ctgggtcggg gctggcgcgc gactacccgc cgctgcagag
     2221 gctcgtgtgg cggtatctgc tgcccgcgct gcgggtgttg ccgggcgtgc gcagcacccg
     2281 cacctccggg cgctatctgg ccacgctggt caacgacgcc cggttcgacg gtgtcagcgg
     2341 cgaatatttc gacgggctgc gaccgatcag gtcgtcggcc gactcctacg accgcggcaa
     2401 ggcgctcgac ttgtgggaga ccagcgagcg gctgctggcg cacgcgatca agtgacgcag
     2461 cggttttcgg tattgtcggc ggggtggcgc aggaccggcg ggcccgtgtc gcagtcgcgg
     2521 cgctgttcct gaccaacggc gcgctgttcg cgaatctgct gccgcgctac ccagagatca
     2581 agtccgacct gcatctgtcc aatgcgatct acggcgcggc gatcgcggcg ttctccggtg
     2641 gcgcgctggt ggcggggctg gccgccgcgg cgctgatccg gcgatttcac tcctcgcggg
     2701 tcgcggtggc cggcacgatc ggcatcgcgg tgttcgtcat catcgcggca ctggccacct
     2761 cgccgttggc gcttgccgcg gcgctgttcg tggcgggcgc ctcggacgcg atcaccgatg
     2821 tggcgcagaa cgcgcacggg cttcgggttc agcgcaacta tggccgctcg atcatcaact
     2881 cattccatgc ggtgtgggcg gtcggcgcga tcctcggcgg tctgatgggc gccggtgcga
     2941 tcgcgataca catgccgcgg gcgacgcatc tgtcgattgc cggcgcggtg ttcagcgccg
     3001 tggtcgtcat cgcgtacccg tacctgttgc gcgggcccga tcacgacgac cacccgtctg
     3061 cgcggtcggc cggtcgggga ggtgcgggca tcgcggtgta tgccacgctt gtcgcccttg
     3121 tggtgatcgc gatcgcgggc gcaaccgtgg aggatgccgg aagttcttgg gccacactgt
     3181 atttgcgcga tagcctgggc gcgccggggg cggtggcggt gttcggctac gtggcgctgg
     3241 tcgggttcat gttcgtcggc agaatgactg gggaccgatt ggtggaccgt ttcggcgagc
     3301 gtgccgtggt gcgagcgggc gggttcgtcg ccgccgccgg gatgggcgcc gcgctggcgt
     3361 tccccagtgt gcccggcacg atcgcggggt tcgcggccgc ggggctgggt gtggcgaccc
     3421 tgatacccgc cgcgatgcac ggcgccgacc agttgccggg catgcgcccg ggcacgggac
     3481 tgacggcggt gacgtggctg atgcggatcg gcttcttcgg cgcaccgttg cttgtcggcg
     3541 tcgtcgcgga cgcgacgagc ctgcgcgtcg ggttgctgtc cgtgccggta tccggtgtcg
     3601 ttgtcatcgc gcttgcgggt gcgctcagtg cacggcgcca gctatctcga tcgtgagcac
     3661 gccagccacg atgaacgcga tgccgagcac catcacccag gtgagcggtt cggcgaacag
     3721 aaaccgcgct atgacggcga ccagcgcgac gccgcaggcg gtccatacgc cataggcgat
     3781 gcccaccggc atgcccagcg acagcgccac ccacagcaga tagaagcaca gcagatagcc
     3841 cgcgaccacg ggcgcaatcc acaccttctt gcggaacccg tcggaggcgc gcagcgcgag
     3901 cgtcgcgaaa acctcgacga gaatcgcgcc ggcaagcgac agccacatca cgattcgtcc
     3961 gcggcggccc gctgagagcc gaactcgacg agcagcacgc cggcgatgat cagcccgatg
     4021 cccgccacga tcggccacgt gaacggatcg ccgtaaatcg cagcggccag caccgccgtc
     4081 gccgccgtgc cgcacgcacc ccagatgccg taggcgacgc cgaccgccat cccggtccgc
     4141 aggaccatcg tcagcaggat gaacgagccg acgtagccgg ccacgaccac aatcagccag
     4201 gccgaatgat cttgcgacgc ccgcaaagac agcgttgccg cgacctcgac cgcaatcgcc
     4261 gcgatcagca gcacccactt ccgcacgcga ccaaactatg ccaccccgtt ctggctggcc
     4321 ctttcgacca tcggcgtcag cacctcgtgc gcgaaccgcc gcagttcggc ctcgtcgccg
     4381 aagttcggat cggtcggcgg catcgagatg tacgtgagaa acaaccgggc gaacatgtcg
     4441 gccacccaac gcacctgctg tgtcggcgtg ccgggtgcct cgccgtgaat gtagttggcg
     4501 atgaacgccg aacccagctt gagcagttcg acggagtcgg cggccggggc ggactcgaac
     4561 tgtccggcgc gacgcaacat cggatgttgg gtggcgaacc tgacggcggc gatgaacgcc
     4621 tcggccacgc cctcatgcgg atccgcgacg gtatcgatcg cgtcggcaac tgcggcgagg
     4681 aaacgctgcg tttcacgtcg gacgagggcc tcgaccaggt catcgcgtcg cgggtagcgg
     4741 cggtagaccg tcatccggga cacgcctgcg cggcgcacca cgtcttctac ggtaatccgt
     4801 tgcagcccaa cggctgccgc ttcaccaacg gcggcgtcga gaatacgttg gctggtgcgg
     4861 tcggtggatt cgtccacgcg gcctgccgcg gtcgcgccgc cgacgatctc gatcagctgg
     4921 ccgtcgatca cggcgccgcc gcagtggtca gctctgcgcc cgccgcgcgt tggcgcgcga
     4981 gttggacgta gcgcggtgtg ccgaacgccc agcgcagcct gcgcgcgtcg gactgcagcg
     5041 ccggatcgat cagaacgcgg gtcagcgcac tgggacggtc gaagccgatc agttcgcgcg
     5101 cccactgcgg cgcacggctc atgcctgcgt agatcgcgaa gcggtgcagt tggcgatcga
     5161 cggggccggg aaggttgggg aagaacggcg acgtcatgag gaaatcgatg aactcgcgag
     5221 tctgtgcgtt cacgctcagc cgtggccgca tggcctcgac atagtcgtcg agttcagcgg
     5281 ccgtcgtcgg aacccactca gcaccgccca accggccgat caccgcttgc tcagccaggt
     5341 atcgatcccg ttcctgttgc gacagcggcc ttttcgtatg ttcgtaggct cgcatgatca
     5401 tccacggaat gcaggtgtgc acccaggcga tcagctcggg gtccaatgcg cgatacggca
     5461 ccccgtcggg gcgcgtgccg gtgacgaacg aatgcacacc cttgacgcgt tcaatgagcc
     5521 cggtggccgc ctcggtgttg ccgaacgtcg tggtcagcac ataacccagg gtcgctcgcg
     5581 cccgctggaa cgggtcctgg cgatagttcg agaggtcttg gacgcccgcg acgacggacg
     5641 gatgcaggat ctccaacacg atcgcgggga ggccggcctg ggagacagcc gccaggtcgg
     5701 cgttgatctc ccaagacacg ctgtcgggcc cgatcagtcc gggatcgccg ggcctacctc
     5761 cgtacacggc tggatcgtcg tggcggccgg cgcccttctc gaactcggcg acgatgcgac
     5821 tgcgcagatc cattgcggct cctggagcaa atgttacaaa tttcatatca actataccaa
     5881 tcaggtagcg acacgtgaca cggatctccc ggtcacctgc ggtttccctc ggtaacctcg
     5941 agggatgagc actgttgtcg aaaccgccta cggcccaatc cgtggcgtcg acgacggcgg
     6001 tgtgaggtcg tggctggggg ttcgctacgg cgccccgcct accggcggcc tccggtggcg
     6061 cgcgcccgag cagccccagc cgtggaccga cgttgccgat gccagcatcc ttgggcccgt
     6121 ctgccctcag ccggtcgacc cgcggatccc catcgacctg ggcgcgcccc aaggcgacga
     6181 ctgcctgacc ctcaatatct gggcgtcctc ggatatcgcc gctggcgacc gcaagccggt
     6241 gatggtgtgg attcacggcg gcgcctacgt acttggttcg gcgaaccagc cgatgtacga
     6301 cgggcgagtg ctcgcggcgg gcggcgacgt cgtcgtcgtc accgtgaact accggctcgg
     6361 cgcattcgga ttcctagatt tgtcgtcgtt cagcacgcca cgacgccggt tcgaaagcaa
     6421 tctcggactg cgcgacgtgc tgcacgccct gcactggatt cgcgacaaca tcgccgcgtt
     6481 tggcggcgac cccgaccgcg tgacggtgtt cggcgagtcg gcgggcgcgg gcatcatcac
     6541 caccctgctg gcaagcccgg ccgccgaggg actgttcgca ggcgcgatcg cgcagagctc
     6601 accggctact tcgttgtacg acgcgacccg cgggcggcga gtggccgcgc agttcctggc
     6661 cgcgcttggc ctgggggaca acgacatcga gcagttgacg gatgcgccag tcgaggcgct
     6721 cgtcgctgcg tcgcgaacgt tgttcaatga agtcccggtg cagtgtcccg gcacgctggc
     6781 gttcgccccg atcgtcgacg gcgatctcgt ccccgaccac ccggtgaaac tggcccgcga
     6841 gggccggtca catccggtcc cgctgatcat cggcacgaac aagcatgagg cggcgctgtt
     6901 ccggtggatg aagtcgccgc tgatgccgat cacgcccgaa gcgatcaatg cgatgttcac
     6961 ccagatcgcc gcggaacagc cgaatctgga gttgccgacg gacgatcaaa tcgacacggc
     7021 gtatccgggt ctgcggggca aggcccgcgg aatgggagta gcgcgcgatg tcggatttcg
     7081 gatgccgacg gtgtggctcg ccgaagggca cagcaccgtc gcaccggtgt acctgtaccg
     7141 attcgattgg gccacaccga tgttgcggct gctgcggctg tccggcgcgc acgccaccga
     7201 gctgcccttc gtctggggca atctcgtcgc cggtcccaaa gacccgacat tccggttggg
     7261 cggaatgaag accggcaagg tggtgtcggc gcggatccag gcccgctggc gcaacttcgc
     7321 cacccagggc aagccgctcg ggttggacgg tgagcccgaa tggctgccgt accaagcagc
     7381 cgaccgggcc tgcctggtga tcgaccggca agacacggtg ctcaacgacg tcgacctgca
     7441 catccggtcg gcgtggggca gtgaggtgct cagcttccgg taacggcggg cagttaaact
     7501 cgttgcggag gtgtcgtcgt ggacctggca ttctcggatg cgctgccgcc gctgatgcac
     7561 gaccccgtga tgtacgcggt tccgttcttc ctgctgtttc tgatcatcga gtggaccgcg
     7621 gcgcgcaagc tcgcccacgt cgaggccgag cggccaccgt cgggcgcgta tctgaagccc
     7681 gacgcatggg ccagcatctg gatggggctg gtgtcggtcg ggacgagcgg ggtgctcaac
     7741 ttcatcgcgc tgctggggta tgcggcgttg tacgcgtatg tggcgccgtg gcacctacca
     7801 gccacgcagt ggtacacctg ggtgatcgcc atcgtcggcg tcgacttcct ctactactgg
     7861 tatcaccgga tggcccatcg ggtgcgcatc ttctgggcca ctcatcaggc ccatcactcg
     7921 agccagtact tcaacttcgc caccgcgctg cggcagaagt ggaacatcag cggtgacgtg
     7981 ttcttacggg cgctgttgcc gctcttcggc gttccgccgt ggctggtgtt cctgtccttc
     8041 tcgatcaatc tgatctacca gttctggatc cacaccgagc gcatcgatag actttggcga
     8101 ccaatcgaat tcgtcttcaa cacgccgtcg caccatcggg tgcaccacgg catggatcag
     8161 cagtatctgg accgcaacta cggcggcatc ttcatcctgt tcgaccggct gttcggcagc
     8221 ttccaggcgg agaccacgcg accgcactac gggctgacca agcaggtgga caccttcaac
     8281 atctggacgc tgcagacgtt cgagtacgtc gcgatcgccc gcgatgttcg ccacgcgagg
     8341 cgctggcgtg accggctcgg ctacatcttc ggtccaccgg gatgggtgcc tgccgaagat
     8401 cgggtcaata ccggcggaag accggcaccc gtcccgtagt gtccgcgatg tggagacggt
     8461 ctttgacccc gacgtcgacc ctgggctggt tgaacgtgcc cttgcggcaa gcaccttcgg
     8521 ctcgatgtgg ctcgacatcc cgcggccgga gtacccgaag gccaccgggt cggtcatctg
     8581 cgacctgctg gtcgtcggcg gcggctacac cgggctgtgg accgcgttgc atgccgcgca
     8641 acgaaatccg gaccgccgca tcgtcctcat cgaggccaac cggatcggct gggccgcgtc
     8701 gggtcgcaac ggcggcttcg tcgacgccag cctgacacac ggccgcgaga atggaaaggc
     8761 gcgctggccc aacgagatcg acacgctcga ggcgatgggc cgggacaacc tcgatggtat
     8821 gcaggccgat atcgagtcac tcggactcga cgccgaatgg caacgcagcg gcatgctatc
     8881 ggttgcgacg gagccacacc aggtggattg gctcgccgac gcggccgcgc atggagaggg
     8941 ccagctgctc ggcttgcgcg aggtccgcga cgaggttcat tcgccgacct atctggccgg
     9001 cctgtacaac cccgccacct gtgcgatcgt gcatccggcc aagctcgcct tcgaactggc
     9061 ccgtgcctgc cgcgaggccg gggtacaaat ctacgagcac acgaacgcgc aggggctgga
     9121 ctccgccggt gtcgcgctgc acatccaggc gggcgaggcg gctttcacgt gccgcaacgc
     9181 cgtgctcgcc acgaacgtct ttccgagcct gctgcggcgc aaccggtggc acaccgtccc
     9241 ggtatacgac tacgtgctgg ccaccgagcc gctgaccgac gcgcagctcg accgcatcgg
     9301 ctggcggcga aggcagggaa ttggcgactc cgccaaccaa tttcactact accgcctcac
     9361 gatggacaac cggatcgtgt ggggcggcta cgacgccatc taccatttcg gccgcagggt
     9421 cgacgatcgg tacgaggacc gcgccgccac ctatcggcgg ctcgcggcgc acttcttcat
     9481 cacgttccct tcgctcgacg atgtccggtt cacgcaccgc tgggcgggcg ccatcgacac
     9541 caacacccgc ttctgcgcgc actggggact ggcccgcgaa gggcgggtgg cctacgtcaa
     9601 cggattcacc gggctcggcg tcgctgcggc gcggttcgca gccgatgtgt gcctcgatct
     9661 cctcgatggc gagcctacgg cgcgtaccga actggagatg gcgcgcaagc ggccgttgcc
     9721 gtttccgccg gagccgctgg ccagcgtcgg tattcaggcg acgcgatggt cgctggatcg
     9781 ggccgaccac tcggcggggc ggcgcaacat actgctgcgc gcgctggatg cgctgggcct
     9841 cggcttcgat tcctgagtcg aaatccccac gggcacagcg atgatcagta ggctcagcgg
     9901 catgagcgac cgaagacgac aacgagtgag gatcgcagcg cgcaacgagc gaggaccgga
     9961 gcgaggagga gtcgagacat gagcgataga ttgatgaacg gcgagaaact agagcgtggc
    10021 caatcgctga catcccgcaa tggggcgtac accttgacgc tgcaggacga cggcaatctg
    10081 gtgctggccg ctcgcggcga tgccgtatgg gcgaccggca cgaatgggca ggacgtggag
    10141 cgcctcgagg tgcagaagga cggcaactgc gtcctctaca cgtctgacaa gccggtctgg
    10201 cacaccgata ccaagggcgc caaggacgtt cggctcctgc tgcaggacga ccgcaatctc
    10261 gtgctgtacg ggttcgacgg accggcatgg gcgtcaaata cccagacggg tgaggcgccg
    10321 cctgcgccgc ctgctccgcc cgccgccgag gaaccggcag gagagccggt cgccgtcgcc
    10381 gccgaagagc ccgcgccccc tgcgcctgag cccgcagccg cggccgcacc gccacctcca
    10441 ccgccgccgc ccgcaccgcg gacctacacc gtcgtctctg gcgacactct ctgggcgatc
    10501 tcggaacgct tctacggcga cggcagcaag tatcaggtga tcgccgacgc cagcggcatc
    10561 gccaacccgg atctgatcta tcccgggcag gtgctcacga tcccttaact gccagcggtc
    10621 agttcgaccc gttcgcctta cgcaagcggc tgtaatacca ccagggccgg gccggcgcac
    10681 cgttggcgtg gtcgacggcg gcgacgaatg tgtcgagcag gcaccggtcg tggcggcgac
    10741 cgtccatagc gcatagtcgg cgatacaggt gctcggcggt ctgcccgcgc aggtcatccg
    10801 gccgctcgat acctaggcgc aacagcatcc gggcgatcgc aggtccgatg ttgggtaggg
    10861 cccgtaggtc gctttcgctc atgtctcgtt ggtaccgaac ggattctgcg gtgtcttgaa
    10921 ctaatcggac acggcgtcgg tgagcagtgc gacctggtcg agctcgccgg aacacggcag
    10981 caatatgacg tcgtcgcagc ccacctcggc gaggcgctgc agttcgtcgt gcaagcgttc
    11041 gcggccggtg agggcgtcgg cccggacgtc cggaaagtag tcggatccgt agtagtgcat
    11101 caggtagtgg tcggcgatct cgtcggcgtc ggggcccagc gagaaatacc gctccaccac
    11161 gatccggggt tgccccgggc ggccagcgtt gctccaggcg cggcgggccg aagtgatccc
    11221 gtctttgagc accggcatgc cgaatgacgg cgccacccaa ccttcggcgt gcgccgccat
    11281 gcgggcgaac gaagcaggca ccagtccgcc gaccaggacg ctcggtcggc cggttggcag
    11341 cgccggcatc ggacccgagg ccccggagat gtcgccggac cagacttgcc gcatggcggt
    11401 gagcgtgccg tcgaaggtgg cgccgcgtgc ggaaagtgag gcgttactcg ctgagtaatc
    11461 ttccggccaa cctccgagcg ccagcccgac tgttaggcgg ccgccggaaa gctggtcgac
    11521 ggatgcgatt tgcttcgcca agacaatcgg gttacgtcgg tagccgacat tcagcactgt
    11581 ggtgagcatt tcgacccgtt cggtgcgcgc cgcggcggcc gcaagcgcca cgagcgggtc
    11641 gaggttgtcg tagaccagtc gatcgatcac acccagcgag gcgaatccga cgagctcgct
    11701 ctgtgccgcc cacgatccga tcacggttgc atcgacaccc ggcacagcgg ccggcaatcc
    11761 gattccgact ctcatgcgac cagtgtgaga agccgatccg attgacgtct tgaacgtttc
    11821 ggacatttcc gagccgaaag gtccgggccg tcactgcttt acggacgggg gtgtgtaacg
    11881 tcgcacggtc ggaggccgat gagcaattgc gtgccgttat cgcagctcac cggtacgctg
    11941 tacagccttg acacataaag atcggaggcc gtggtgcgcc gactctggtt gcgcatgctc
    12001 gcagcggcgg ccgccccgat cgtcgcgctc tcgttgccat ccgcaccggc gcaagccaca
    12061 gcgggcgtgc cggtgttccc cggcatggaa gttcgtcaag gcaccaacgt ctgcacactg
    12121 ggctacgtcg acccgcaaat gcgaatcgca ttcaccgccg gccactgcag gggcagcgga
    12181 caggtgaccg acagggacgg caatgcgatc ggcagccagg cggtgttccg cgacaacacg
    12241 ccgaacggcg ccacgatcga caccaatcat cagatcgccg actgggaggc gatcagcctt
    12301 gcccccgacg tcgcggtcaa caacatcctg cccgtcggac ggcagctgat cgccgatccg
    12361 gccgtcgtgc cggtggtcgg tctgccggtg tgtcacttcg gggtgatcac gggggagagc
    12421 tgcggcacca tcgaggccgt caacaacggt tggttcacga tggccaacgg cgtggtgagt
    12481 cagaagggcg actccggcgg accggtgtac aacctcaccc ccgacggcag ggcggcgatc
    12541 gtcgggatgt tcaacagcac ctggggtcag tacccggcgg cggtctcgtg gcaggccgcc
    12601 agcgaacagg ctcgtgagga cgtcatctcc gccgcgtcag cgcccgcgcc ctaacggagc
    12661 agctcgaaga ccgggataga cggtgcgaca gcgcgtattt cgtcgtcggt cgactgcggc
    12721 gtcaagccgc cgacgtgccc cttgacctcc cagtaccacc gctcgagata gcgcttcagc
    12781 agctgcggtt tggcgtcgtc ggcgatttcc gagatgcgca cgtgccgcgt gcgccaccgc
    12841 ggtcccatct cgacggaccc cgccgtacgc gcgttgcggg cccattgcgt gttaccgcgc
    12901 ggcgacacga catagtcgcg gccatcgacg gtgagcaggt tgatcacgac gccgcgcagc
    12961 ttgccggtct tacgcccgcg gacccggagc gcgcgtgttc cggcgaggct gatcccggct
    13021 tcggccagcc agcggatcag ttcgttgcct gcgcgagcgg caaggttggg tgcgtcgtag
    13081 cggacggtca tcgaggttcc ttttcaatga gagagcggtg ctctcttaaa gattggcacg
    13141 cccgcggcag gaaatcaaga gcagtgatct cgtttcgtgt cacactggcc gtcatgggca
    13201 agcggcagga gacgcgcgag aagatcgagg cgcagatcgt ggagctcggc aggcgccatt
    13261 tggtgacgga cagtgcggcc gggctgtcgc tgcgggcgat cgctcgcgac ctcggcatgg
    13321 tgtcgagtgc ggtgtaccgc tacgtggcca gccgcgatga cctgctgacg ttgctgcttg
    13381 tcgacgcata ttcggaactc gccgacgcgg tcgatcgcgc cgcggcgacc gcaggcgggt
    13441 catggcgcga tcgtgtcctc gcaacggcgc acgcggcccg cgcatgggcg atcgatcagc
    13501 ccgcccgctg gtcgctgctg tacggcagcc cggtaccggg ttaccacgcg ccgcgagagc
    13561 gcaccgtcgg gccggggacc cgggtggtcg cttcgctgtt cgacgcgatt ggggccggca
    13621 ttgcggcggg ggatatccct cagtccaatg ttgctgtggc ccaaccgttg tcgtcagact
    13681 tcggccggct gcgcgaagag ttcgcgttcg ccggcgacga ctccgcggtg gccaagtgct
    13741 ttttgctgtg ggccggactc gtcggtgcga tcagcctgga agtgttcggt cagtacggcg
    13801 ccgacacgct gacggaaccc ggggccgttt tcgacctcca ggtccggctg ctgatcaaca
    13861 cgctgagcat ctaaatgtag acttgactta tataagtgaa agcttctact ttggaagagt
    13921 gcacgcattc gacgtcctgg gcgacccggt acggcgtcga attctggaac tgctcgtcga
    13981 cggcgagaag tcggcgggag ccatcggagt aaccattcag ggcgagttcg cgatcacgca
    14041 gccggcggtg tcgcagcatt tgaaggtgct gcgggacaac gggtttacct cggttcggcc
    14101 cgacggtcag cggcggctgt acgcggtgag cgggcgagcc ttgcgcgatg tcgacgagtg
    14161 gctcgacggg ttccgtcgct tctggacacc acatctcgac gcgctgcaca ccgaaatcgc
    14221 ccggggtaaa cgacaacgac gaaggggcgc aacatgattg agattgacgt cgaccatcag
    14281 atcaatgccg ttcagcgtaa gctcgggcac cgcacgatcg acgccgggga ggcgcacgtc
    14341 ctcactgtga gtcagtctta cgacaccgac caggacgacc tttgggatgc ggtcaccaat
    14401 atcgagcgca taccccgctg gttcttgccg atctccggcg acctgcaatt gggcggctcc
    14461 tatcagttgg agggccaggc aggaggaaag atcctgacgt gcgatccgcc gaaaaacttc
    14521 accgcgactt gggaattcgg cggcaacgtc agctggatcg acgtcacgat caccggcgac
    14581 ggccccgacc gcgcgcgact ggtacttgaa cacatcgccc aagtcgacga cgacgagatc
    14641 tggcgccagt tcggccccgg cgccgtcggc atgggttggg attccatgtt gctcgggttg
    14701 gccctccacc tggctaccgg cgaatccatc gacccgtcgt tcggacagca gtggacgggc
    14761 accgacgacg gccgtcgctt cctgacgctg tccggcgacg agtggtatgc cgtgaacgtc
    14821 gccttcggga cggatcccgc ggcagcgcgc gcgatggcgg atcgctgtct caaggcctac
    14881 ctcggcgagg aataaactag aacacgttct agccaggcgg gcacggcggc gatattctca
    14941 aggcgatgcc tgaccagaca cctgtccaga ttgcctgggt cacacgcgac ctcgatgcca
    15001 ccgaaaaggc gctgaccacg ctattaggcg ccaagaagtg gatccgcctg cccggtgttc
    15061 acttcggtcc cgacgcgtgc acgtatcgcg gtcgacccgc ggacttcgtc gccgacatct
    15121 cgctgagcta tgccggcgac acccagctcg agctcatcgc accggtggca ggcgagagca
    15181 tctacaccga gttcctgaac cgcgcgggag ccggcctgca ccacatctgc atcgaggccg
    15241 acgacgtcga gcgcgcggtg gccgagcgtg acgccgacgt ggtgcagcgc ggtgtgatgc
    15301 ccggcggcat ggagttcgcg tacatctccg ccgacgatgc cggcgtgccg tacatcgaga
    15361 tcgcgtacat ctcgcccgag attcgggcgt tcttcgacta catcaagcag gagcagcagt
    15421 gagaccgacg agcgcttgcg cgaggagcaa tcgatgacgc agatcccgga aaccgttaac
    15481 gccgcaggcg tgacgtcgtg gtccgacgag gtcgacgtcc tggtgatcgg gtttggcatc
    15541 gcaggtggct gcgcggcggt cagcgcggcc gcggcgggcg cgcgggtgct ggtgttggag
    15601 cgcgcagcag ccgcgggcgg gacgacttcg atggcgggcg ggcatttcta tctcggcggc
    15661 ggcaccgccg tgcagcaggc caccggccat gacgacagcc ccgacgagat gtacaagtac
    15721 ctggttgcgg tgtcgcggga acccgagcac gacaagatcc gcgcctactg cgacggcagc
    15781 gtcgaacact tcaactggtt ggaggacttg ggctttcagt tcgagcgaag ctacttcccc
    15841 ggcaaggccg tcatccagcc gaacaccgaa gggctgatgt tcaccggcaa cgagaaggtc
    15901 tggccgttca aggatcaggc ggtgcccgca ccccgagggc acaaggtgcc ggtgccgggc
    15961 gacaccggcg gggcaggcat ggtgatcgac ctgctgctca aacgcgcgga cgaactcggc
    16021 gtgcagatcc ggtacgaaac aggcgccacc gcactggttc tcgacaatgg cgccgtcgtc
    16081 ggcgcgacct ggaagcgctt caacgacacc ggggcgatcc gggccaaggc cgtggtgatc
    16141 gccgcgggcg ggttcgtgat gaacccggag atggttgccg agttcacccc gaagctggcg
    16201 gagaagccgt tcgttctcgg caacacctac gacgacgggc ttggcatccg gttgggcgag
    16261 tcggccggtg gcgcgaccaa gcacatggac caggttttca tcaccgcgcc ggcgtatccg
    16321 ccgtcgctcc tgttgaccgg gatcatcgtc aacaagctcg ggcagcgctt cgtcgccgag
    16381 gactcctacc attcgcggac ctcgggtttc gtgatggacc agcccgacag cgcggcattc
    16441 ctgatcgtcg atgaggagca cttggaacgg cccgaggtgc cgttggtgcc gctgatcgac
    16501 gggtgggaag ccgtcgagga aatggaagcc gcccttggca tcccggaggg aaacctggtt
    16561 gccacgttga accggtacaa cgagtacgcc gcccgcggcg aggatccgga ctttcacaag
    16621 cagccggaat tccttgcgcc gcaggacaca ggaccttggg gcgcgtttga tctgtcgctg
    16681 ggcaaggcga tctacgcggg cttcactgtc ggcgggctgg ccacctccgt cgacggccag
    16741 gtgcttcacg aggacggcag cgtgatcccc ggcctctatg cggccggtgc gtgcgcgtcg
    16801 aacatcgccc aggacggcaa gggctacgcc agcggaaccc agctgggcga gggctcgttc
    16861 ttcggccgcc gggccggagc gcacgcagcg cgcgcctaga ccggtgcgag gcgccactgg
    16921 ccgccgccga gcagttccag gcgtcggtcg tggtgctgtt cgaccgtggg gcggtgactg
    16981 acgctgacca cgatgcagtc cggcagcgcg gtgcgcaacg accggtacag cgcgaactcc
    17041 tggccctcgt cgagcgccga ggtcgactcg tcgaggaaca ccaccttggg cttggcgagc
    17101 agcacccgcg cgaacgcgat gcgctgctgt tcaccggggg agagcacctt ggcccagtca
    17161 gcaacctcgt cgaggcgccc ggcaagatga ccgagcgcca cggtgtcgag cgcggattgg
    17221 attgccgcgt cgtcgaattg gcccgcagca cccggataag agatcaccgt acgcagatcg
    17281 cccagcggca tgtacggcag ctgcgacaag aacatggtgc cgtcggccgg accccggacc
    17341 gtgcccgacg tgaacggcca gagctgggcg aggctgcgca acaacgtggt cttgccgcag
    17401 ccggagccac ccgtgatcac caatgattcg cccgagtcca gccggacgtc gagcgggtcg
    17461 atcagccgtg tcccgtccgg ggtgcggacc tcgacatcgt cgagctcgag cgagccgtcg
    17521 ccgctcggga gggccgtcaa agtcggtagg tcccttgcct tttcattcga ggtcaccaac
    17581 ccgtcgagcc gcaggatcgc cgcccggtag ttggcgaacg agtcgtacac cgctctgaac
    17641 cacgacagcg agtcgtgcaa ctggcggaac gcacttgacg actgagtgac gtcaccgaag
    17701 tcgatctcgc ctgcgaacat ccgcggagcc tgtacgacag tgggtagcgg gtcgacgatc
    17761 cggcttatcg accggttcca gccgaggaac atcagcgtcc ggcggacgaa ggcccggtag
    17821 ttggcgatg
//
LOCUS       DASRJA010000005         2712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2538362,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000005 DASRJA010000000
VERSION     DASRJA010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2538362"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..275
                     /locus_tag="VFC01_00225"
                     /pseudo
     CDS             1..275
                     /locus_tag="VFC01_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
     gene            complement(330..1637)
                     /locus_tag="VFC01_00230"
     CDS             complement(330..1637)
                     /locus_tag="VFC01_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN78105.1"
                     /translation="MFSETRYAINGDLRVAYRASAEGPRDIVYVPNWHSCCELVMEVP
                     STQGWIEAMTSLGRLIFFDQPGTGASDPVTARALPTLEQWADSITAVLDDLGSSEAVL
                     VASNAAFAPAALFAATHPSRTTALVVLDGYADADGTRLVRDEIKGAIAMWGSGELQHL
                     VNPDMPWNEEIRSAWARLERLAVSPRTLDLVGPLMSELDVRAFLPTVRVRTLVIQHSD
                     SPFVPPAKGKYIAGHIPDAEYVEVPGRNWHHIVEPWRDSFREIAEFLTGEQAEVADDR
                     VLATVLFTDIVDSTRHAAEMGDRDWHALLDAHDAVVRAQFARFRGREVNTSGDGFLAM
                     FDGPQRAIRCAMAIRDAVQTLGIEVRAGLHTGECEIRGADIGGIAVHIGARVTALAGP
                     NDVLVSSTLRDLVIGSGIEFDDRGAHQLKGVPGEWHLFAVASG"
     gene            1780..2070
                     /gene="rpsF"
                     /locus_tag="VFC01_00235"
     CDS             1780..2070
                     /gene="rpsF"
                     /locus_tag="VFC01_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZN78106.1"
                     /translation="MRPYEIMVILDPTLDERTVAPSLEAFLNVIRKDGGSVDKVDIWG
                     KRRLAYEIAKHAEGIYAVVDVKAEPATVSELDRQLNLNESVLRTKVLRTDKH"
     gene            2183..2695
                     /locus_tag="VFC01_00240"
     CDS             2183..2695
                     /locus_tag="VFC01_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZN78107.1"
                     /translation="MAGDTTITVVGNLTADPELRFTPSGAAVANFTVASTPRIYDRQS
                     GEWKDGEALFLRCNIWREAAENVAESLTRGSRVIVQGRLKQRSFETREGEKRTVVEVE
                     VDEIGPSLRYATAKVNKASRSGGGGGGFGGGGGGGGSRPAASDQPKDDPWGSAPASGS
                     FSGSDDEPPF"
BASE COUNT          474 a          873 c          914 g          451 t
ORIGIN      
        1 ccgtgttggt tgcgatcgac gtagcgttcg tcggacatca acgcggcgaa gtccgcagcg
       61 acaacggaat gctgggcatg atggtcgaca aggcgttcac cgaactcacc caaccggatg
      121 tggtggtgtt ccccggcggc atcgggacgc gtgtgctgat caacgatgac gaggtgctcg
      181 actcgacccg cgaggcgcac gagcattcac ggttcaccac atcggtgtgc accggcgggc
      241 tggtgctggc ggcggcgggg ttgttgaccg ggttgcttgt gcgcactttc ccgcgctgag
      301 cgcggaattc tgcacacaac agctgtttgt cagccggatg cgacggcgaa cagatgccac
      361 tcgccgggca cgcctttgag ctgatgcgcg ccacggtcgt cgaactcgat ccctgacccg
      421 atcacgaggt cgcgcagtgt gctggacacc agcacgtcgt tcggccctgc cagtgcggtt
      481 acgcgcgcgc cgatgtgcac ggcgatcccg ccgatgtcgg cgccgcggat ttcgcactca
      541 ccggtgtgca gcccggcgcg cacctcgatg ccgagggtct gcaccgcgtc gcgaattgcc
      601 atggcgcagc ggatcgctcg ttgcgggccg tcgaacatcg cgaggaagcc gtcgcccgag
      661 gtgttcacct cgcgaccgcg gaagcgtgcg aactgggcgc ggacgacggc atcgtgagcg
      721 tcgagcaggg cgtgccaatc acggtcgccc atctccgccg cgtgacgggt cgagtccacg
      781 atgtcggtga agagtacggt ggcgagcacg cgatcgtcgg ccacctcggc ttgctcgccg
      841 gtgaggaatt cggcgatctc ccggaaggaa tcgcgccacg gttcgacgat gtggtgccag
      901 ttgcggcccg gcacctcaac gtattccgcg tcgggtatgt gcccggcgat gtacttgccc
      961 ttcgccggtg ggacgaaagg gctgtcggag tgctggatga caagagtccg cacacggacg
     1021 gtcggaagga acgcccgcac gtccagttcg gacatgagag gccccaccag atccagcgtc
     1081 ctcgggctga ccgccagacg ttccagccga gcccacgctg accggatctc ctcgttccac
     1141 ggcatgtccg ggttcaccag atgttggagc tccccgctgc cccacatagc gatcgcgcct
     1201 ttaatttcgt ctcgaacgag tcgggttccg tctgcgtctg cgtaaccgtc gagcacgacc
     1261 agcgcggtgg tgcgtgaggg atgtgtcgcc gcgaacagcg cagcgggcgc gaacgcggcg
     1321 ttcgacgcga cgaggaccgc ttcgctgctc ccgaggtcgt cgagcacggc ggtgatgctg
     1381 tcggcccact gctccaacgt cggtagcgcc ctggctgtga cggggtcgga cgctcccgtg
     1441 cccggctggt caaagaagat gagtcgtccg agcgaggtca tcgcctcgat ccacccttga
     1501 gtggacggga cctccataac aagctcgcag cagctgtgcc agttcgggac gtacacgatg
     1561 tcgcgaggcc cttcagccga cgcgcggtac gcgacgcgta agtccccgtt gatcgcatag
     1621 cgcgtctcgg agaacacggc ggaagtgtat gcccgcgagc agcgcgattt cgcagttcag
     1681 cgggcattcc tgtagcctgg gccggttgcc gacgcaggcg accctcctgc cacggaacga
     1741 ccgtggccgc actagaccgt aggaggtgat gggttcctta tgcgtccata cgaaatcatg
     1801 gtcattctcg accccactct cgacgagcgc accgttgctc cgtcgctgga ggcattcctc
     1861 aatgtcatcc gcaaggacgg cggcagtgtc gacaaggtcg acatctgggg taagcgccgg
     1921 ctggcttacg agatcgccaa gcatgccgag ggcatctatg ccgtcgtcga tgtgaaggcg
     1981 gagcccgcga ccgtgtccga gctcgaccgt cagctcaacc tgaacgagtc cgtactgcgg
     2041 accaaggtgc ttcggaccga caagcactaa agggtgccgg tcgtcggagc cgctgcgtag
     2101 gctcgcctgc gactgacttc gcgcacaccg ccagcgatga gcgctcgcgc gaagagccga
     2161 caaacttgcc caggaggaac tcgtggctgg tgacaccacc atcaccgtcg tcggaaacct
     2221 gaccgccgac ccggaactgc gcttcacccc atcgggtgcg gccgtcgcga acttcacggt
     2281 cgcgtcgacg ccgcgcatct acgaccgcca gagcggggag tggaaggacg gcgaggcgct
     2341 gttcctacgc tgcaacatct ggcgtgaggc cgccgagaac gtggccgaaa gcctgacccg
     2401 cggctctcgg gtgatcgtgc agggccggct caagcagcgc tcgttcgaaa cccgtgaggg
     2461 cgagaagcgc acggtggtcg aggtcgaggt cgacgagatc ggcccgtcac tgcgctacgc
     2521 gaccgccaag gtgaacaagg cgagccgtag cggcggcggt ggcggtggat tcggcggcgg
     2581 cggtggcggc ggaggttccc gcccggcggc atccgaccaa cccaaggacg acccgtgggg
     2641 cagcgccccg gcatcgggct cgttctccgg aagcgacgac gaaccgccct tttagacaac
     2701 aactttcaat ca
//
LOCUS       DASRJA010000006        12174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1667012,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000006 DASRJA010000000
VERSION     DASRJA010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667012"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..331)
                     /locus_tag="VFC01_00245"
                     /pseudo
     CDS             complement(1..331)
                     /locus_tag="VFC01_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214758.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            841..978
                     /locus_tag="VFC01_00250"
     CDS             841..978
                     /locus_tag="VFC01_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78108.1"
                     /translation="MWIVEVNFAGRRFTHEVHGHRRPFFHFSPRNFSWRPSQLRDPHA
                     A"
     gene            1049..1621
                     /locus_tag="VFC01_00255"
     CDS             1049..1621
                     /locus_tag="VFC01_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78109.1"
                     /translation="MLISAAEVMRERGAAGVTIDEVLARSGAPRGSVYYHFPEGRNQI
                     LTEALQYAGEALTELIGDAAEKGGMYLVRKFVTFWEDLLVESDFTAGCPVVAAAIGSA
                     DDEPQLTSVAGSIFRRWRDALTGAFVSDGFDEPCAASLAITCIASLEGAVVLCRSTRS
                     VDPLREVAEQVEFLVKSREFVRRYGIPDHR"
     gene            complement(1618..2139)
                     /locus_tag="VFC01_00260"
     CDS             complement(1618..2139)
                     /locus_tag="VFC01_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZN78110.1"
                     /translation="MATVYFTASSLDGYIVDEAESLDWLTSRNIDANGPFGYEAFIKD
                     VGALVMGSATYEWVVRNHPGDWMYEQPSWVLTHRPEIIADGHPVQTFAGAVAELHPKL
                     VSAAAGKDVWVVGGGDVAAQYAAVGLIDEMIVTYAPCSLGGGSRVLPIRSEWTLVESG
                     GNGDFVCARWRKA"
     gene            complement(2141..3058)
                     /locus_tag="VFC01_00265"
     CDS             complement(2141..3058)
                     /locus_tag="VFC01_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN78111.1"
                     /translation="MTDPARRDRLRELLDAVVDADNTDVGDMARSSFASEFHFSREVR
                     RLTGESPAALRRRIMLERAAWRLRRGESVSAVATDEGWSSAEVFSRAFSRAFGLPPSR
                     ASDIGFRLPAPNGLHFHPPGSLWLDSDGDTKEPDISQLMVAHDVADTAYLINQAAQLS
                     KEQWTEEISPGQVILDWDGPEPSVGAVLGAIVWTKEVWLATIEGRDFPSREATEPAST
                     PAQQLATHHDELGKRWAAMVSEYRAEGRLGDTVIDALCDPPESFQLYGIVAHVLTYSA
                     HRRGLARMMLARHGVRTALGDPLNWMRGN"
     gene            complement(3138..3749)
                     /locus_tag="VFC01_00270"
     CDS             complement(3138..3749)
                     /locus_tag="VFC01_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78112.1"
                     /translation="MNTADTRVSVVSAIAIGAVLLALSTPLVHAAPGDGPGSDNTPPP
                     PGQVAVAEPLEISVSENPVVFLPHQTTKTINFTWTPQPDSKVWVHVTEGSGAKEVWSK
                     VMDKGLTGPLNLEVDYGSFYWIWVCREVHDTCPLAVVTTERLDINVPPLERQLPLPEP
                     PGDNDRSTPQQTDGTGGQGPQRRPGDGSQEHGVDAADQQTRQE"
     gene            3916..5055
                     /locus_tag="VFC01_00275"
     CDS             3916..5055
                     /locus_tag="VFC01_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN78113.1"
                     /translation="MSDFDALCANDGELAQLRTSIRDFLAADRAEFGWQPAVDSWLAK
                     WDPDFSARLAAAGFVGLTIPADYGGLGLGYLHRYVVTEELLAHGAPVAAHWIADRQVA
                     PALLSYGTEEQRGRLLPQIAAGRLYSAIGMSEHGAGSDLAAVQTRATRTDGGWLLRGS
                     KVWTSGAHVAHQIVVLARTSPLDPEHRHAGFSQFIVLTSAPGLTIEPVVLMNGEHHFN
                     EVLFEDVFISDEDVLGQIGDGWHQVTAELGFERSGPERILSTATLIFSAIRALGDVDD
                     GVAADVGDLVARMISLRQLSISVARTLADGGDAATRAALVKDLGTRFEQDSVELVADL
                     LDYVQDAEPLRALLADARVHSPLFTLRGGTNEVLRGVVAKGMGVQ"
     gene            5052..6029
                     /locus_tag="VFC01_00280"
     CDS             5052..6029
                     /locus_tag="VFC01_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN78114.1"
                     /translation="MNELAQLVDELCDRSTGDELWRNLEDTGLARLTSGQGAGPAEAA
                     IVLSGLARHAAAAPIAETDLLAVWLADKAGVSIPDTGPLTVAMADTAEIDGRLRGTVH
                     DVPWPLSTVVLAVRTADALHVAAVEDAQAVDGHNLAGEPRGSVAFDMAVGDMHAVDPG
                     VADELIRRGAWARCNQIVGALDAACDLTVGHTGERVQFGRPLSKFQAVQHSLAAMAGE
                     IERARAAAALAVAAAADYGFAHPATDYAVTAAKVAVGRAVNPVTTIAHQLHGAIGVTA
                     EHRLWRTTMRARSWVDEFGTTTQYARRLGRMALAAGDPWDVVVSYPAAR"
     gene            complement(6007..6852)
                     /locus_tag="VFC01_00285"
     CDS             complement(6007..6852)
                     /locus_tag="VFC01_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HZN78115.1"
                     /translation="MTTEVTDAVDVAAYFDRIGYQGPAEPTVETVQALVAAHNRSIPF
                     ENLDPVMGIPVADLSAAALTDKLVHRHRGGYCYEQNGLMGYVLEELGFGVERLAGRVV
                     WLNPDGPLPAQTHHVLSVAVPEVDGRLLVDVGFGGQTLTSPIRLEAGPVQQTRHEPYR
                     IRERGDGYQLEAEVRGEWQPLYTFTARPQPRIDLEVGSWYVSTHPDSFFVTGVTGALV
                     TDDARWNLRGRNLAIHRAGETEKIRFDTAAEVLDALTDRFGIDLTDLGDPADVEARVN
                     ELLDS"
     gene            6910..7482
                     /locus_tag="VFC01_00290"
     CDS             6910..7482
                     /locus_tag="VFC01_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78116.1"
                     /translation="MPAHLTEELREDLLARWSETHRKHHTVAHLHEMLDAVGLLAESG
                     IEFDREAVELAAWFHDAIYEIGRNDNEDRSAELARELLESSPIRDEVARLVLVTKTHK
                     VDDDDINGAVLSDADLSVLASEPIRYRAYAAAVREEYADVPDEVFKPARAQVLSALLT
                     GPLFHTATGRERWEDRARRNITEEIAELTG"
     gene            complement(7998..8780)
                     /locus_tag="VFC01_00295"
     CDS             complement(7998..8780)
                     /locus_tag="VFC01_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZN78117.1"
                     /translation="MTGVTDNSENVVLVEQRDRILIITINRPEAKNAVNAAVSRGLAD
                     AVDRLDSDPGLSVGIVTGAGGSFCAGMDLKAFARGENVVVEGRGMGFTERPPVKPLIA
                     AVEGYALAGGTEVALATDLIVASKESAFGIPEVKRGLVAGGGGLLRLPQRIPYAIAME
                     LALTGENLPAQRAHELGLVNVLAEPGGALDAAIALAERITANGPLAVAATKRIITESR
                     GWSPEEMWTQQTRILMPVFSSNDAKEGAIAFAEKRPPKWTGS"
     gene            complement(8802..10097)
                     /locus_tag="VFC01_00300"
     CDS             complement(8802..10097)
                     /locus_tag="VFC01_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78118.1"
                     /translation="MALTLVDWGGELPEEFVHEILARTVRLKGIDMPGIARVLEVLHT
                     GHFGVVVSEWIHGGALGKIAETAPSPTSVAGAMQSLAAAAEAAHRAGLVLSLDDPARL
                     RLGLDGHAALAFPATMPEATAASDLHGIGQAMRVLLNSDEQAPFLISTTVSGLQEGGI
                     ASAATLLTLLREATGNDVESGRVMPPLPPPVPGRYADFRNFGAEDQAAAARRQVMRIA
                     LVAAAVMVVMAMVAAGSTLNRFLDNDHDSVGFNADKLGLSGPTASASQPPPDVVRKRA
                     APEEQVQPVTAVVFSPGGSPDSPDAAGAAVDGNPATAWSTDTYYDADPFPKFKPGVGL
                     LVRLARPAAISAVMVDMNSTGTVVQVRAAASDSPKALAETIELTPPTPMRPGRNRIQV
                     ASSDPISNVLVWISTLGSTDGKSRAAISEIELQAATPPA"
     gene            10342..11151
                     /locus_tag="VFC01_00305"
     CDS             10342..11151
                     /locus_tag="VFC01_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN78119.1"
                     /translation="MPATDIEPSELTKWDPGLTERVMSITRPFLKRYFRSQVRGLDNM
                     PSGGALLVSNHSGGMLPMDVPILAAHFYEKHGYDRPLYTLSHDMLSVGPTGDFFKKIG
                     YISANHENADEALRSGGLVVVFPGGDYDVYRPTLSENKIDFGGRTGYVKAALNAGVPI
                     VPTVGIGGQETQIFLSRGTWLAKRLGPIARLARTKIVPISFGFPFGLSVVIPPNIPLP
                     AKIVMRVLPPIDIVAEFGENPDIDEVDAHVRHFMQRALDELAKERTLPVLG"
     gene            11157..>12174
                     /locus_tag="VFC01_00310"
     CDS             11157..>12174
                     /locus_tag="VFC01_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN78120.1"
                     /translation="MAITDRLTETLGIVATMGRARLLAPMRPDKYLRIASAMQRENMG
                     ITSGFAAAAQRCPDRTGLVDELGALTWREIDQRADALAAGLQALPDGAAPTVLGIMAR
                     NHRGFVESLIAANRIGADVLLLNTSFAGPALAEVVQREDVDAVIYDEEFTETVDRALA
                     DKPDATRIIAWTDGPQDGLTVEKLIADHAGREPQRASSKSKVILLTSGTTGTPKGAKH
                     SGGADGGPGILKAILDRTPWRAEETVVIVAPMFHAWGFSQLVFAASMACTVVTRRKFD
                     PEATLELIDRHRATGLCVVPVMFDRIMELPDDVRNKYSGRSLRFAAASGSRMRPDVVI
                     RFMDQ"
BASE COUNT         2037 a         4036 c         3981 g         2120 t
ORIGIN      
        1 agcagggcac ggcgcacgtc gggatgatgg acgatcgtga cgcgtgccgc ccccttgtcg
       61 gtcatctgcg tcaggtcggc gccctgcacg cgggtccgcg cgtagtccaa cgcgttcaga
      121 tagccggtcg acagcgtcgc aatcgccttg gtgccggcca tcattcgcgc atactcgatg
      181 accttgaaca tctgcgcgat gccgctgtgg tgtcaccgac caaccagccg acggcgggac
      241 ggtcatgttg accgaaggtg agctcgcatg tgggagaaac cttcaggccc atcttgtgct
      301 cgagatcgcg cacgttgctc ttgtagtgcc ccatcgtcac gggctcaccg tcggactgtg
      361 gccgaccgac actcggggcc tcctgggttt cggctggttc tgcgcgaagc ctgtgcaggt
      421 tccgaggtgt ccggagacgc acgggacgcc attcatcgtc ggcaccgggc gaccggagac
      481 gaccgtcggc gccccgttgt acccgttctg attcaacggt tcggcgccgg cgatcggagc
      541 ggcgatgaga gccgcggcgg tcatacctat gagcagtgac ctgcacagat gcgagtgctt
      601 ggccatggca gccccgcttt cctcggttct gccgtcagcg tacggacccg aagtacggct
      661 gagctgcgat ttcgcaatta tgtcccgtca cataatcgca tgtcaggcgc tgatcactaa
      721 tatcggctaa ctaccctcgt tcacctgctc ctaggggccc aggggtgtga tggggaatac
      781 atctattgcg cacatgtgtt accgcttcta tgctgttggt catagacagg gaggcacccg
      841 atgtggatcg ttgaggtcaa cttcgccggc cgccggttca cgcacgaggt gcatggacac
      901 cgtcgaccgt tcttccactt cagcccgcgc aacttcagtt ggcggccgtc gcagctgcgc
      961 gacccgcacg cggcctgata ctcgctggag gtttgttctg tccaccgcga ccaagaccag
     1021 gaccaaacgg ggctcgacgc gcaccaacat gttgatcagc gccgccgagg tgatgcgtga
     1081 gcgcggagcc gccggcgtca caatcgacga ggtgctcgcc cgcagcggcg cgccgcgcgg
     1141 ctcggtgtac taccacttcc ccgaggggcg caatcagatc ctgaccgagg ccctgcagta
     1201 cgccggcgag gccctgacgg aactcatcgg cgacgccgcc gaaaagggcg ggatgtatct
     1261 cgtcaggaag ttcgtcacgt tctgggaaga cttactcgtc gagagcgact tcaccgctgg
     1321 ttgtcctgtg gtggccgcgg cgatcggctc ggccgacgac gaaccacagc tgacgtccgt
     1381 cgcgggcagc atcttccgcc gctggcgcga cgcgctcacg ggcgcgtttg tgagcgacgg
     1441 cttcgacgag ccctgcgccg cgtcgctggc catcacatgc atcgcctccc tcgagggagc
     1501 ggtggtgctg tgccggtcca cccgcagcgt cgatccgttg cgcgaggttg ccgagcaggt
     1561 cgaattcctg gtcaagtcaa gggaattcgt gcgtcgctac gggattcccg accaccgtta
     1621 agccttgcgc caccgggcgc agacgaagtc gccgttgccg ccggattcca ccaacgtcca
     1681 ctcggaccgg atcggtagca cgcgagagcc gccacctaac gagcacggtg cgtacgtgac
     1741 aatcatttcg tcgatcaggc cgacggctgc gtactgcgcg gccacatctc ccccgccgac
     1801 gacccacaca tccttacccg ccgccgcgga caccagtttc ggatgcaact cggcgacggc
     1861 accggcgaag gtctgcaccg gatgcccgtc ggcgatgatt tcgggacggt gcgtcaacac
     1921 ccacgacggc tgctcgtaca tccagtcacc cggatggttg cggaccaccc actcgtacgt
     1981 cgccgacccc atcaccagtg cgccaacatc tttgatgaac gcctcgtatc cgaacgggcc
     2041 attcgcatcg atgttgcggg acgtgagcca gtccaggctc tcggcctcat cgacgatgta
     2101 gccgtcgagg ctggacgcgg tgaagtagac agtcgccatc tcaatttcct ctcatccagt
     2161 tcagtgggtc accaagggca gtgcgaaccc cgtgacgggc aagcatcatg cgcgcgagac
     2221 cgcgtcggtg cgccgagtag gtcagcacat gcgcgacgat cccgtacagc tgaaacgact
     2281 cgggcgggtc gcacaaggcg tcgatgacgg tatcccccaa ccggccttca gctctgtatt
     2341 cggacaccat tgcggcccag cgctttccga gctcgtcgtg atgggtggcc aactgctgtg
     2401 ctggtgtcga cgcaggctct gtggcttctc gactggggaa gtcgcggcct tcgatggtgg
     2461 ccagccacac ttccttcgtc cacacgatcg caccgagtac cgcgccgacg ctcggttcgg
     2521 gaccgtccca gtcaagaatc acctgccccg gtgagatctc ctctgtccat tgctctttgg
     2581 acaactgtgc ggcctggttg atcaagtagg cggtatcggc gacgtcgtgg gccaccatca
     2641 attgagaaat atcgggctcc ttggtgtcgc catcgctgtc cagccacagc gatccgggcg
     2701 ggtggaagtg caggccattc ggggcgggca gccggaaccc aatgtccgag gcgcgcgacg
     2761 ggggaagacc gaacgcgcga ctgaaggcgc gggagaacac ctccgccgac gaccagccct
     2821 cgtcggtggc caccgcggat accgactcac cgcggcgcag gcgccacgcc gcgcgctcca
     2881 gcatgatgcg tcggcgcagc gccgccgggg actcaccggt cagccgacgc acctcccggg
     2941 agaagtggaa ttccgaggcg aagctgctgc gtgccatgtc accgacgtcg gtgttgtcgg
     3001 cgtcgacgac agcgtcgagc agttcgcgca atcgatcgcg gcgtgctgga tctgtcacgt
     3061 tcaccgagta tcgtcggccg cccgcacgat ggacatgaca attcctgctc agctgaggat
     3121 gtcgacatgt cgcggggtca ttcttggcgt gtttgttggt cagcagcatc aactccatgc
     3181 tcttgtgaac catctccagg tcgtcgctgg ggcccctgcc cgccggttcc gtcggtctgc
     3241 tgcggggtcg acctgtcgtt atctcccggc ggctccggca acggcagctg ccgctccaga
     3301 gggggcacgt tgatgtcgag ccgctccgtc gtgacgacgg ccagcgggca ggtgtcgtga
     3361 acttccctac acacccaaat ccagtagaac gagccgtagt cgacctccag gttcagcggg
     3421 ccggtcaatc ccttgtccat caccttgctc cacacctctt tcgcccctga accctccgta
     3481 acatgcaccc acactttcga atccggttgc ggtgtccagg tgaagttgat ggtcttggtg
     3541 gtctgatggg gcaggaagac cactgggttc tcgctgacgg aaatctcaag cggctccgcc
     3601 accgccacct gcccaggcgg tggaggtgtg ttgtcgctgc caggaccatc ccctggcgct
     3661 gcatgaacga gcggcgtact gagtgcaagc aggaccgctc cgatggctat cgccgaaacg
     3721 accgatacgc gggtgtctgc ggtgttcata atgtgctccc ttggcgatga cgcgggcccg
     3781 cgatgatccg cgctcatcgg taagagcagc cagcccccgt tccaagtacg ctttttcggc
     3841 cacagttttg ggcgaccgtt caggacggat atgtcatgag ctaccagctc gccgctgact
     3901 agactctggt ggccagtgag cgacttcgac gcgctatgcg ccaacgacgg tgagctggcc
     3961 cagctgcgga cgtcgatccg cgacttcctc gccgccgatc gcgccgagtt cggctggcag
     4021 ccggccgtcg actcctggct cgcgaagtgg gacccggact tttccgccag gttggccgct
     4081 gcagggttcg tcggcctgac gatcccggcc gactacggcg gcctcggact gggttacctg
     4141 caccgctacg tcgtcaccga ggaactgctg gcgcacggcg ccccggtcgc cgcgcactgg
     4201 atcgccgacc gccaggtcgc gcccgcgctg ctgtcgtatg gcaccgaaga gcagcggggc
     4261 cggctcttgc cgcaaatcgc agcgggacgg ctttactcgg cgatcgggat gagcgaacat
     4321 ggcgcgggat cggacctcgc cgccgtgcag acgcgggcga cccgaaccga cggcggctgg
     4381 ctgctgcgcg ggtcgaaggt ctggacgagt ggcgcgcacg tcgcgcatca gatcgtcgtg
     4441 ctggccagaa ccagcccgct ggatcccgag catcggcatg cgggcttcag ccagttcatc
     4501 gtgctgacgt cggcaccggg cctcacgatc gagccggtcg tgttgatgaa cggcgagcac
     4561 cacttcaacg aggtcctctt tgaggacgtg ttcatttccg atgaggacgt gctgggtcaa
     4621 atcggtgacg gctggcatca ggtcaccgcc gaattgggtt tcgagcgaag cggtccggag
     4681 cgaattctgt ccacggccac gctgatcttc agcgcgattc gcgcgttggg cgatgtcgat
     4741 gacggcgtcg cagccgacgt gggagacctt gtggcccgga tgatttcgct gcggcagctg
     4801 tcgatctcgg tggcgcgcac gctggccgat ggcggagatg ctgcaacccg ggcggcgctg
     4861 gtgaaggatc tcggcacccg cttcgaacag gactccgtcg agttggtcgc cgacctgctg
     4921 gattacgtcc aggacgccga gccgttgcgt gcgttactcg ccgatgcgcg ggtgcactcg
     4981 ccgctgttca cactgcgggg tgggacgaac gaggtgctgc gcggcgtggt cgccaagggg
     5041 atgggcgtgc aatgaacgag ctggcacagc tggtcgacga actctgcgat cgatcgacgg
     5101 gcgacgaatt gtggcgcaat ctcgaggaca ccggactcgc ccggctgacg agcggtcagg
     5161 gcgcaggccc cgcggaggcg gcaatcgtgt tgtccggctt ggctcgacat gcggccgcgg
     5221 ctccgattgc cgagaccgat ctgctcgcgg tgtggctcgc ggacaaagct ggcgtgtcga
     5281 ttcctgacac cggcccgctg acagtcgcga tggccgacac cgccgaaatc gacggtcggc
     5341 tcaggggaac cgtacacgac gtcccgtggc ccttgagcac ggtcgtcttg gcggtgcgga
     5401 ccgctgacgc gctgcacgtc gctgcggtcg aggatgcgca ggcggttgat ggccataacc
     5461 tggccggcga gccgcgcggt tccgtcgcct tcgacatggc ggttggcgac atgcacgcgg
     5521 tcgatcccgg cgttgccgac gagttgatcc gccgcggcgc gtgggcgcgg tgcaaccaga
     5581 tcgtcggtgc gctcgacgcg gcctgcgacc tcaccgtcgg ccacaccggc gagcgcgtgc
     5641 agttcgggcg tccgctcagc aagttccagg cggtgcagca ttcgctcgcc gcgatggccg
     5701 gcgagatcga acgcgcccgc gctgcagcgg ctttagcggt cgcagccgcg gctgactacg
     5761 gtttcgccca cccggcgacc gactacgcgg tgacggcggc caaggtggcc gtcggacggg
     5821 ccgtgaatcc ggtgacgacg attgcccatc agttacatgg cgcgatcggc gtgacggccg
     5881 agcaccggct gtggcggacg accatgcgtg cacgcagctg ggtcgacgag ttcggcacca
     5941 caacgcaata cgcgcggcgg ctgggccgga tggcgctggc cgccggggat ccgtgggatg
     6001 tggtggtcag ctatccagca gctcgttgac gcgcgcttcg acgtcggctg ggtcaccgag
     6061 gtcggtcagg tcgatgccga agcggtcggt caacgcatcg agcacctcgg cggcggtgtc
     6121 gaagcgaatc ttctcggtct cgcctgcgcg gtggatcgca aggtttctgc ctcgcaggtt
     6181 ccagcgagcg tcgtcggtga ccagtgctcc cgtcacgcct gtgacgaaga acgagtcggg
     6241 atgcgttgac acataccaac ttccgacctc gagatcgatg cgcggctgcg ggcgggcagt
     6301 gaacgtgtac agcggctgcc actcgccgcg gacctcggcc tcgagctggt agccgtcacc
     6361 gcgctcccgg atgcggtacg gctcgtgtcg agtctgctgc acgggtccgg cctcaagccg
     6421 gatcggcgac gtcaacgtct gcccgccaaa tccgacgtca accagtagcc gcccatcgac
     6481 ttccggcacc gccaccgaga gcacgtgatg tgtctgtgcg ggcagcgggc cgtccggatt
     6541 cagccatacc acgcggccgg cgagccgttc gacgccaaag cccaactcct cgaggacata
     6601 tcccatcagc ccgttctgct catagcagta gccgccgcgg tggcggtgca cgagcttatc
     6661 ggtgagagcg gcggcgctca ggtcggcgac ggggataccc atcaccgggt cgaggttctc
     6721 aaacgggatg gaccggttgt gcgcggccac gagcgcctgc accgtctcga ccgtcggctc
     6781 ggcggggcct tggtagccga ttcgatcgaa atacgcggcg acgtcgacgg cgtccgtcac
     6841 ttccgttgtc atgccgccat cctgcgacct caataggggt tcaggtcaag ccggtgggac
     6901 actgaggacg tgccagccca cctgaccgag gaactgcgag aggacttgct ggctcgctgg
     6961 tcggagacgc accgcaagca ccacaccgtc gcccatctgc acgagatgct cgacgcggtc
     7021 gggctgctgg ccgaatcggg gatcgagttc gaccgtgagg cggtggagct ggcggcatgg
     7081 ttccacgacg ccatctacga gatcggccgc aacgacaacg aggaccgctc ggccgagctg
     7141 gcgcgcgagc tgctggagtc atcgccgatc cgcgatgagg tcgcgcgact ggtgctggtg
     7201 accaaaacgc acaaggtgga tgacgacgac atcaacggag cggtgctcag cgacgccgac
     7261 ctgtcggtgc tcgccagcga gccgatccga taccgcgcct atgccgcagc ggtccgggag
     7321 gagtacgccg acgtgccgga cgaggtgttc aaaccggctc gggcgcaggt actttcggca
     7381 ctgctgacgg ggccgctgtt ccacacagcg acgggtcgcg agcggtggga agaccgcgct
     7441 cgacgcaaca tcaccgagga gatcgccgaa ctcaccggct aattgtttgc caacgaaagt
     7501 gcggggatag cgtccctccc agctgtgtgc catgctgcgg gagtggttgc ctcctacgag
     7561 gagttcgctg cagaacaccg gacccagcac ctcacgccgt tcaaccgctg gtgcgcagtc
     7621 gtcggtaact ccatggtgcc tgtcgcggtg gttacggcgc tgacgggtcg actcagggct
     7681 ggagccgcga tcttcgctct ggcgaacaca atcgttgtcg cggggcacgt cgttgaaggc
     7741 aacttgccgc gagctgcgcg tgacctcgtg cggcatccca tctggggtgt gcgcgcggat
     7801 gtcgctgtgg caattgcgac gatcagagac ttaatgcgat agcagcccgg ccgagctcac
     7861 cagttagatg tcattctccg cggcgtcatc aaatgaatga cgccgtcagc atcagtagtc
     7921 cctttgtgaa gacaagtagg ggcggcgggg ctcggcggcc gtcgactcct tgaccgcaac
     7981 tgcgcggccg aaaaccgtca gctgcccgtc cacttcggcg ggcgcttctc ggcgaacgcg
     8041 atcgcgcctt ccttcgcgtc gttactgctg aagacgggca tcaggatcct tgtctgctgg
     8101 gtccacatct cttccgggct ccagccgcgc gactcggtga tgatccgctt cgtcgccgcg
     8161 accgccagcg gcccgttggc ggtgatccgt tcggcgagcg cgatcgcggc gtccaaggcc
     8221 ccacccggtt cggccagcac gttgaccaac cccagctcgt gtgcccgctg cgccggcagg
     8281 ttctcaccgg tcagcgcgag ctccatcgcg atcgcgtacg ggatgcgctg cggcagccgc
     8341 agcagcccac cgccaccggc gaccagccca cgcttcacct ccggaatgcc gaacgccgac
     8401 tccttcgacg ccacgatcaa atcggtggcc agcgccactt ctgtgccacc tgccagtgcg
     8461 tacccttcga ccgccgcgat cagcggcttg acgggcggac gctcggtgaa ccccattccg
     8521 cggccctcga ccacgacgtt ctcgccgcgg gcgaacgcct tgaggtccat gcccgcacag
     8581 aacgagccac ccgcgccagt gacgatgccg acagagagtc ccggatcgct gtcgaggcgg
     8641 tccacggcgt cggccaaccc gcgactcacc gcggcgttga cggcgttctt ggcctctggc
     8701 cggttgatcg tgatgatcag gatccggtcg cgctgctcaa ccaggactac attttcgctg
     8761 ttgtctgtca cgccggtgat gctatcggct gaccgcccgg gtcacgccgg cggcgttgct
     8821 gcctgcagct cgatctcgga gatcgcggcg cggctcttgc catccgttga gcccaacgtg
     8881 gaaatccaca ccagaacatt cgagattggg tctgagctgg cgacctgaat tctgttgcgc
     8941 cctggccgca ttggggtagg cggcgtgagt tcgatggttt cggccaacgc cttgggcgag
     9001 tcactcgcag cggcacgcac ctgaacgacg gtgcccgtgc tgttcatgtc gaccatcacc
     9061 gcgctgatcg cggcgggtct tgcgagtcgc acgagcagtc cgacgccggg cttgaatttc
     9121 gggaacggat cggcgtcgta atacgtgtcg gtggaccacg ctgtggccgg attgccgtcg
     9181 acggccgcac cggccgcgtc cgggctgtcg ggcgatccgc cgggcgagaa caccaccgcg
     9241 gtgacgggtt ggacctgctc ctctggtgcg gctcgcttgc gcaccacgtc cggcggcggc
     9301 tggctggccg aggccgtcgg cccgctcaac cccagcttgt cggcattgaa cccgacagag
     9361 tcgtgatcgt tgtcgaggaa cctgttcagc gtcgagcccg cggccaccat cgccatgaca
     9421 accatcacgg ccgcagccac cagcgcgatc cgcatgacct ggcggcgggc ggccgcagcc
     9481 tgatcctcag ccccgaagtt gcggaagtcg gcatagcgac ccggaaccgg cggtggcagc
     9541 ggcggcatca cccggccact ctcgacatca ttgccggtgg cctcacgcag tagcgtcaga
     9601 agcgttgctg cgctggctat tccgccctcc tgcagccctg acactgtggt ggagatcagg
     9661 aacggcgcct gctcgtcgga gttcagcagc acgcgcatag cctgaccaat tccatgcagg
     9721 tccgacgcag cggtcgcctc cggcatggtg gcgggaaaag ccagcgccgc gtgaccgtcg
     9781 aggcctagcc gaagccgggc gggatcatcg agggacaaca caaggccggc gcggtgggcc
     9841 gcctcggctg cagcggccag cgattgcatc gcgcccgcga cgctcgtcgg cgacggcgcc
     9901 gtctcggcga tcttccccag cgcgccgccg tggatccatt ccgacaccac cacgccgaag
     9961 tgtccggtgt gtagcacctc gagcacccgg gcgatgccag gcatgtcgat gcctttcagc
    10021 cggacagtcc gcgcgaggat ttcatggacg aactcttcgg gcagctcacc gccccagtcg
    10081 acaagcgtga gagccacttc ttggtccgtc gccaggtcga gcccatgcca gaactgcaac
    10141 tgcggtcgcc cgccgtgaga ggccaacagt cggtagcgac catgcccgac actcacgccg
    10201 ggagctaggt tggagggact gccggttgtc tggtcgcgtt cggtcgtcat tgcccgtcga
    10261 ggtagccctg cataggcggt tctaaaccgg tcggccgctc gggtacgata actagaacac
    10321 gttctaattt tagggggacc cgtgcccgca accgatatcg aaccgtcgga gctcaccaag
    10381 tgggatccgg gcctcaccga gcgcgtcatg agcatcaccc ggccgttcct caagcgctac
    10441 ttccgctcgc aggtcagagg gctcgacaac atgccgtccg gcggcgcgct gctggtcagc
    10501 aaccactccg gtggcatgtt gccgatggac gttccgatcc tcgcggctca cttctacgaa
    10561 aagcacggct acgaccggcc gctatacacg ctgagccacg acatgctctc ggttggaccg
    10621 acgggcgact tcttcaagaa gatcggctac atcagcgcca accacgagaa cgccgacgaa
    10681 gcgctgcgct ccggcggcct cgtcgtggtc ttccccggcg gtgactacga cgtctatcgc
    10741 ccgacactgt cggaaaacaa aatcgacttc ggcggccgca ccggctacgt gaaagccgcg
    10801 ctgaacgccg gcgtgccgat cgtgccgacg gttggcatcg gtggccaaga gacgcagatc
    10861 ttcctctccc gtgggacctg gctggccaaa cgcctcgggc ccatcgcacg tttggcgcgg
    10921 accaagatcg tgccgatctc gttcggcttc ccgttcggcc tgagcgtggt gatcccaccc
    10981 aacattccgc taccggccaa gatcgtcatg cgggtgctgc cgccgatcga catcgtcgcc
    11041 gagttcggcg agaaccccga catcgacgag gtcgacgccc acgtgcggca cttcatgcaa
    11101 cgtgcccttg acgaactggc caaggagcgc acgctccctg tgcttggcta aggcccatgg
    11161 ccatcaccga ccggctcacc gaaacgctcg gcatcgtcgc gacgatgggt cgcgcccgcc
    11221 tgctcgcgcc gatgcggccc gacaaatacc tacggatcgc ctcggcgatg cagcgcgaaa
    11281 acatgggcat cacatcggga ttcgccgccg cggctcagcg ctgccctgac cggaccggtc
    11341 tcgtcgacga gcttggcgcc ctgacctggc gcgaaatcga ccagcgcgct gacgcattgg
    11401 ccgctggact gcaggcgctg ccggacggtg cggctccgac ggtcctcggg ataatggcgc
    11461 gcaaccaccg cggcttcgtc gagtcgctga tcgcagccaa ccggatcggt gccgacgtgc
    11521 tactgctgaa tacgtcgttc gccgggcccg cactcgccga ggttgtgcag cgggaagacg
    11581 tggacgccgt catctacgac gaggagttca ccgagaccgt cgaccgcgcg ctggccgaca
    11641 aacctgacgc cacgcggatc atcgcctgga ccgacggacc gcaggacggg ctgacggtcg
    11701 agaagttgat cgccgaccat gctgggcgcg agccgcagcg ggcgagcagc aagagcaagg
    11761 tcatcctgct gacgtccgga acaacgggaa ctccgaaggg cgccaagcac tccggtggcg
    11821 ccgacggcgg acccggcatc ctcaaggcga tcctggaccg cacgccatgg cgtgccgaag
    11881 agacggtcgt catcgtggcc ccgatgtttc acgcgtgggg cttctcccag ttggtgttcg
    11941 cggcgtcgat ggcgtgcacg gtagtcaccc gacgcaagtt cgaccccgaa gccaccctcg
    12001 aactcatcga ccgccaccgc gccaccgggc tgtgtgtcgt gccggtgatg ttcgaccgga
    12061 tcatggagct gcccgacgat gtgcgcaaca aatacagcgg ccggtcgctg cggttcgccg
    12121 ccgcgtcggg ttcacgaatg cgcccggatg tcgtcatcag gttcatggat cagt
//
LOCUS       DASRJA010000007         6448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3660545,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000007 DASRJA010000000
VERSION     DASRJA010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3660545"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VFC01_00315"
     CDS             complement(<1..840)
                     /locus_tag="VFC01_00315"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HZN78121.1"
                     /translation="MYRALRRVLFLVAPERIHTWVFAFLRAVTAVIPARRLLARGLAP
                     HDPVLAITVFGVRFAGPLGLAAGFDKDGRGLNTWGALGFGYAEVGTVTAQAQPGNPPP
                     RMFRLPEDRALLNRMGFNNHGAGELALQLARHTADVPIGVNIGKTKVTPSEAAVEDYA
                     ESARLVGPLAAFVVVNVSSPNTPGLRDLQAISSLRPILAAVKAETSAPVLVKIAPDLS
                     DDDVDEIADLAVELGLAGIVATNTTVSRDGLATPDAGSFGAGGISGPPVARRSAEVLR
                     RLYR"
     gene            complement(840..1088)
                     /locus_tag="VFC01_00320"
     CDS             complement(840..1088)
                     /locus_tag="VFC01_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 family protein"
                     /protein_id="HZN78122.1"
                     /translation="MTAMHRGRMPPGWDTDLSDEYEWIPLRLPPDLTRISASTRLSIE
                     AEYRGWELTRVRLYTDGSRRVLLRRKKSAVDRLDQPGL"
     gene            complement(1085..2068)
                     /locus_tag="VFC01_00325"
     CDS             complement(1085..2068)
                     /locus_tag="VFC01_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78123.1"
                     /translation="MLAISVLSACSSKSGDAPPPTIVPAQAAQSPPVTGPPAGVVRPL
                     GGLAQAAVFDQATASLAVLSPLFESQSMVTVVPMSGQPRPVTLPAPATAMTGDNDGRV
                     YASTRGGYFRVDLRAGTASRVDVEGQPGTDFTAIARRADGKLALGSADGAVYTLGSDS
                     TVGASLKIFARVDALVAQGNTVVVLDRGQTSVTTISASGNDADHALRAGEGATTMAAD
                     SAGRVLVADTRGEELLVFGTDPLILRQRYPVREAPYGLAGSSKLAWVSQTAINTVVGY
                     DLATGIPVEKVRYRTVQQPNSLAFDEASGTLYVVSGSGAGVQVIANAGGRG"
     gene            2249..2560
                     /locus_tag="VFC01_00330"
     CDS             2249..2560
                     /locus_tag="VFC01_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78124.1"
                     /translation="MTLAADRDLGNREFAIEDITTGVHASGFGQVGDGRTFSFHIEKH
                     MLVVEVYRPRVSGPVPQAEDVVALANRKLTDIDLTDERSVAAAVRDAVADAQPVTRTA
                     R"
     gene            2606..3436
                     /locus_tag="VFC01_00335"
     CDS             2606..3436
                     /locus_tag="VFC01_00335"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZN78125.1"
                     /translation="MSWLQVVVLSVLQGLTEFLPVSSSGHLAIASRIFFDDDAGASFT
                     AVTQLGTELAVLLYFARDIGRILKAWVSGLFVAAHRTLDYWMGWYVIIGTVPIGALGL
                     LFKDEIRTGARNLWLIAGVLLVFSAVIAAAEYYGSQKRHAEELNWKDSVIVGLAQCLA
                     LVPGVSRSGATISAGLFRGLDRELAARFGFLLAIPAVFASGLFSLPDAFHPVGDGMSA
                     SGPQLLIATVIAFVIGFLAVAWFLRFLVRHSMYWFVGYRLLLGAVVIALLATGVVSAT
                     "
     gene            3433..4149
                     /locus_tag="VFC01_00340"
     CDS             3433..4149
                     /locus_tag="VFC01_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZN78126.1"
                     /translation="MTVILLRHGRSTSNTAHTLAGRTDGVDLDDKGREQAQAIVERLA
                     GLPIRAIVRSPLLRCRRTVEPLAAALGLDPVVDERISEVDYGSWTGRKIGDLVKEPLW
                     GVVQQQPSAAVFPGGEGLAHVQSRAVAAVREHDRRLTELHEGDALWVACTHGDVIKAV
                     VADALGTHLDSFQRITADPASISVIRYTALRPFVIHVNHTGAALTAGLLAKPSKQDGE
                     ADKKDGEVPPEDAVVGGSTD"
     gene            4204..4791
                     /locus_tag="VFC01_00345"
     CDS             4204..4791
                     /locus_tag="VFC01_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HZN78127.1"
                     /translation="MARAIHVFRTPDRFIAGTVGQPGNRTFYLQAVHDKRVISVVLEK
                     QQVAVLAERIAALLLEINRRFGTPIPPDTGDIEDLSPLITPVDAEFRVGTMGLGWDSE
                     AQTVVVELLAVSETEFDASVVLDDAEEGPDAVRVFLTPESARQFAARSNRVISAGRPP
                     CPLCDEPLDPEGHICVRTNGYRRGALAGSDDDADS"
     gene            4775..5611
                     /locus_tag="VFC01_00350"
     CDS             4775..5611
                     /locus_tag="VFC01_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HZN78128.1"
                     /translation="MTPIHSGGQSPRHPDEVCLRGELTVIGRIRSASNATFLCEAHLG
                     ELQAHCVYKPIAGEAPLWDFPDGTLAGRELGSYLVSDALGWNIVPYTIIRDGPAGLGM
                     LQLWVDQPGDEVGDEPASGPDLVDLLPAGHIPPGFLPVLQAYDYAGDEVTLVHADDIR
                     LRRMAVFDVLINNADRKGGHILCGVDGHVYGVDHGVSLHIDDKLRTILWGWAGKPVDD
                     DTLEAVARLRDQLRGGLSEQLREHITRREVAALHARTIALLDNPVMPTPDRRRPIPWP
                     AF"
     gene            5611..6378
                     /locus_tag="VFC01_00355"
     CDS             5611..6378
                     /locus_tag="VFC01_00355"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HZN78129.1"
                     /translation="MSLTDAALAADVAADAGRLLLSVRERVGFDDPYFLGDAGDKSAN
                     ILILDRLSRDRPDDAVLSEEAVDDVSRVHADRVWIVDPVDGTREFSWYGRTDWAVHVA
                     LWQRSGGPVGGITDAAVALPARGEVYRTDTVVAPPPRTDGPIRITASASRPPAVLWRL
                     RDRMDIQMVRIGSAGAKAMAVVRGDVDAYVHAGGQWEWDSAAPAGVVQAAGLHASRLD
                     GSPMIYNRRDPYLPDFLMCRAELADVLLDGIWSAYRE"
BASE COUNT         1045 a         2181 c         2163 g         1059 t
ORIGIN      
        1 gcggtacagc cggcgcagca cctcagcgga gcgccgcgcc accggcgggc ccgagatccc
       61 gcctgctccg aagctgccgg catccggtgt ggcgagcccg tcgcgcgaca ccgtggtgtt
      121 cgtggcgaca atgcccgcca agcccaattc gactgccaga tcggcgatct cgtcgacatc
      181 gtcatcggat aggtcgggtg cgatcttgac cagcacgggc gccgacgtct ccgccttcac
      241 cgcggcgagg atcgggcgca gcgaggagat ggcctgcaaa tcacgcagcc ccggcgtgtt
      301 cggtgagctg acgttgacga cgacaaacgc ggccaatggc ccgaccagcc ttgcgctttc
      361 ggcgtagtcc tcgaccgcgg cctccgacgg cgtcaccttg gtcttgccga tgttgacccc
      421 gatcggcacg tcggccgtgt gccgggccag ttgcagcgcc agctcacccg cgccgtggtt
      481 gttgaagccc atgcggttga gcagcgcgcg gtcctcgggc agccggaaca tccgcggtgg
      541 cggattgccg ggctgcgcct gtgcggtgac ggtgccgacc tccgcatagc cgaagcccag
      601 cgcgccccag gtgttgagcc ccctgccgtc cttgtcgaac cctgccgcca ggcccagcgg
      661 gcccgcgaac cgcacgccga acaccgtgat ggcgagcacc ggatcgtgcg gcgcaagtcc
      721 gcgcgccaac aaccgacgcg caggaatgac cgccgtcacg gcccgcagga acgcaaaaac
      781 ccacgtgtga atgcgttcgg gggcgaccag aaacagcacc cgccgcagtg cgcggtacat
      841 cacaggccgg gttgatccag tctgtccaca gcagacttct tgcggcgcag cagcacccgc
      901 ctgctgccgt cggtgtacag ccggacccgg gtgagttccc acccgcgata ctcggcctcg
      961 atcgacagtc gggtggacgc gctgatccgg gtcagatccg gcggcagccg aagcggtatc
     1021 cactcgtact cgtcggacaa atcggtgtcc cagcctggtg gcatccgccc acggtgcatc
     1081 gccgtcaccc acgacccccc gcgttggcga tcacctgcac acccgctccc gatccggaca
     1141 ccacatacag ggtgccagac gcttcgtcga aggccaggga gttgggctgc tgcacggttc
     1201 gataacgcac cttttccacg gggataccgg tagccaaatc gtagccaacc acggtgttaa
     1261 tcgccgtctg cgacacccag gccagcttcg acgagccggc cagcccgtat ggggcttcac
     1321 gcaccgggta ccgctggcgc aggatcagcg ggtcggtgcc gaacaccagc agctcctcgc
     1381 cacgggtgtc ggcgaccaac acccgaccgg cggaatccgc ggccatcgtg gtcgcgccct
     1441 cgccggcacg cagcgcatga tcggcgtcgt tgccgctcgc cgagatggtc gtcaccgacg
     1501 tctgacctcg gtccaacacg acaactgtat tcccttgtgc gacaagggca tcgacgcgcg
     1561 caaagatctt caaagaggca ccgacagtgg agtccgaacc caacgtgtag acggcgccgt
     1621 cggcactgcc gagggccagc ttgccgtcgg cacgccgggc gatcgcggtg aaatcggtgc
     1681 ccggttggcc ctcgacgtcc acccggctgg ccgtgccggc gcgaaggtcg acccggaaat
     1741 agccgccgcg cgtcgacgcg tacacccggc cgtcgttgtc accggtcatc gccgtcgccg
     1801 gtgcaggcag tgtcaccggc cgcggttggc cggacatcgg cacgacggtc accatggatt
     1861 ggctctcgaa aagggggctg agcaccgcca acgacgccgt cgcctggtcg aacacggccg
     1921 cctgcgcgag cccgcccagc ggccgcacca ccccggcggg tggtccggtg accggagggg
     1981 actgcgcggc ctgggcaggc acgatcgtcg gtggcggggc atcgccggac ttcgatgagc
     2041 aggccgacag cacagaaatt gccagcaccg taaaagtgac acaaacaacc agaaaatggc
     2101 ggtttagacg cggcggcaag gggtagctct cggtcgtgcg aatagtgttg ctaacaaatt
     2161 gcaagtgtag gcagaccgct catcgccaaa tacaagccgc tcgccgtgcg aggtcactcg
     2221 ggcgcatctc cagcggtatg gtgcgatcat gaccctcgcc gcagacaggg atctgggcaa
     2281 ccgagagttt gctatcgagg acatcaccac tggtgtacac gccagcggat ttggccaggt
     2341 gggcgacggt cgcacctttt ccttccacat cgaaaagcac atgctcgtcg tcgaggtgta
     2401 ccggcctcgg gtctccgggc ccgtcccgca agccgaggac gtcgtggccc ttgccaaccg
     2461 caagctcacc gacatcgatc tcaccgacga gcgcagcgtg gccgcggcgg tgcgcgatgc
     2521 ggtggccgac gcgcagccgg tgacacgcac agcgcgctga cagcagtccc acggtacggt
     2581 cgtcggcgtg atcgaggcgc cggcgatgtc gtggctgcaa gttgtcgtcc tgtcggtgtt
     2641 gcagggattg accgagttcc tgccggtttc gtcgtcgggt cacctggcga tcgcgtcgcg
     2701 catcttcttt gacgacgacg cgggggcgtc gtttaccgcg gtgacccagt tgggcaccga
     2761 gttggcggtg ctgctgtact tcgcgcgcga catcggtcgc atcctcaagg cttgggtgag
     2821 cggtcttttc gttgccgcgc accgcacact ggactactgg atgggctggt acgtcatcat
     2881 cggtaccgtc ccgatcggtg cgttgggatt gttgttcaag gacgaaatcc gcactggcgc
     2941 acgcaatctg tggctgatcg cgggcgtgtt gctcgtcttt tccgcagtga tcgccgccgc
     3001 cgagtactac ggcagtcaga agcgccacgc cgaggaactc aactggaaag acagcgtcat
     3061 agtcggcctc gcgcaatgcc tggcgttggt gcccggcgta tcccggtcgg gcgcaacgat
     3121 cagcgccggc ttgttccggg ggctcgaccg cgaactggcc gcccggttcg gcttcctgct
     3181 cgccattccg gccgtgttcg cgtcggggct gttctcactg cccgatgcgt tccatcccgt
     3241 cggcgacggg atgagcgcca gcggcccgca actgttgatt gccacggtga tcgcgttcgt
     3301 catcggcttt cttgcagtgg cctggttcct gcgctttctg gtgcgccaca gcatgtactg
     3361 gttcgtcggc taccgcctcc tgctcggcgc ggtcgtgatc gcactgctcg ccaccggggt
     3421 agtctccgcg acatgacggt catcctgctg cggcacggca ggtcgacctc gaacaccgcg
     3481 cacacgctcg cgggccgcac cgacggtgtc gacctcgacg acaaggggcg cgagcaggcg
     3541 caggcgattg tcgagcggct ggccgggctg ccgatccggg cgatcgtgcg ctcgccgctg
     3601 ttgcgctgcc ggcgcacggt cgaaccgctg gcggccgcgc tcgggctgga tccggtggtc
     3661 gacgagcgca tctccgaggt cgactacggc agctggaccg gtcgcaagat cggtgacctg
     3721 gtcaaggaac cgctctgggg agtggtgcag cagcagccca gcgcagccgt ctttcccggt
     3781 ggcgaagggc tggcccacgt gcaatctcgt gcagtcgcgg cggtgcgcga acatgaccgc
     3841 aggctgaccg aactgcacga gggcgacgcg ctgtgggtgg cgtgcaccca cggcgacgtg
     3901 atcaaagcgg tggtcgccga cgcactgggc acgcacctcg acagctttca acgaatcacc
     3961 gccgacccgg cgtcgataag tgtgatccgt tacaccgcgt tgcgtccgtt cgtcatccac
     4021 gtcaaccaca ctggtgctgc gctcaccgcc ggactgctgg ccaagccgtc gaagcaggac
     4081 ggggaggccg acaagaaaga cggcgaagtg ccgccagaag acgctgtggt cggtggctcg
     4141 accgattgag gcctgtgcgt ggaattacgg ccgacacgcc gataccggta ttttggaggt
     4201 gccatggccc gcgcaattca cgtcttccgc acacccgacc ggttcatcgc cgggaccgtc
     4261 gggcagccgg gcaaccgcac cttctacctg caggccgtgc acgacaaacg cgtcatctcg
     4321 gtggtgctgg agaagcagca ggttgccgtg ctggccgaac ggatcgcggc gctgctgctg
     4381 gagatcaacc ggcgcttcgg cactcccatc ccgcccgaca ccggcgacat tgaagacctg
     4441 agcccgttga tcacgcccgt cgacgccgaa ttccgggtgg ggaccatggg gctgggctgg
     4501 gattcggagg cgcagacagt cgtcgtcgag ctgctcgcgg tgtctgagac cgagttcgac
     4561 gcatcggtcg tgctcgacga tgcggaggaa gggcccgacg cggtgcgggt gttcctgacg
     4621 cctgagtcgg cgcggcagtt cgcggcgcgt tccaatcgtg tcatttcggc cggccgcccg
     4681 ccgtgcccgt tgtgcgatga gccgctcgat ccggagggcc acatctgcgt gcgcaccaac
     4741 ggctatcggc gcggcgccct tgccgggtcc gacgatgacg ccgattcata gcgggggcca
     4801 aagcccccgt catcccgatg aggtgtgcct acgcggcgag ttgaccgtca tcggacgaat
     4861 ccgctcagcg agcaacgcca ccttcctgtg cgaagcgcat ctcggtgagc tgcaagcaca
     4921 ttgcgtgtac aagccgatcg ccggcgaggc cccgctgtgg gacttccctg acggaactct
     4981 ggcaggccgg gaactgggtt cctacctcgt ttccgatgcg ctcggctgga acatcgtgcc
     5041 ctacaccatc attcgcgacg ggcccgcagg cctcggcatg ctgcagctgt gggtagatca
     5101 gcccggcgac gaagtcggcg acgagcccgc atccgggccc gatctcgtcg acctgttgcc
     5161 cgcgggccat attccaccgg gcttcctgcc ggtgctgcag gcatacgact acgccggcga
     5221 cgaagtcacc ctggtgcatg ccgacgacat tcggctgcgt cgcatggcag ttttcgatgt
     5281 gctgatcaac aacgccgacc gcaagggcgg tcacatcttg tgcggtgtcg acgggcacgt
     5341 gtacggcgtc gaccacgggg tgagcctgca catcgacgac aaactgcgca caattctgtg
     5401 gggctgggcc gggaaacccg tcgacgacga caccctggag gcggtggccc ggctgcgaga
     5461 tcagctgcga ggcggcctca gcgaacagtt gcgtgagcac atcacccgtc gcgaggtagc
     5521 tgcactgcac gccagaacca ttgcgctgct ggataacccg gtgatgccca cgccggaccg
     5581 gcgccgaccc atcccgtggc cggcgttctg atgagcctga ccgatgcggc gctggccgcc
     5641 gatgtggccg ccgacgccgg acgactgctg ctttccgttc gcgagagggt tggcttcgac
     5701 gacccgtact tcctcggcga cgccggcgac aagagtgcca acatcctgat tctcgaccgg
     5761 ttgagtcgcg accgccccga cgacgcggtg ctgtccgagg aggccgtcga cgacgtgtcg
     5821 cgcgtgcacg ccgaccgggt gtggatcgtc gacccggtcg atggcacgcg cgagttctcc
     5881 tggtatggcc gaacggactg ggcggtgcat gtcgcgctgt ggcagcgcag cggcgggccc
     5941 gtcggcggca tcaccgacgc cgcggtcgcg cttccggcgc gcggcgaggt ctaccgcacc
     6001 gacaccgtcg tcgctccgcc gccgcgcacc gacggtccga tccgcatcac cgccagcgcc
     6061 agcaggccgc ccgcggtgct gtggcggttg cgcgaccgga tggacatcca aatggtgcgg
     6121 atcgggtcgg cgggcgccaa ggcgatggcg gtggtccgcg gcgacgtcga cgcgtacgtc
     6181 cacgccggcg gccagtggga gtgggactcg gccgccccgg ccggagtggt gcaggccgcc
     6241 gggctgcacg cttcacggct ggacggctcc ccgatgatct acaaccggcg cgacccgtac
     6301 ctgcccgact tcctgatgtg ccgcgctgag ctggcggatg tcctgctcga cggcatctgg
     6361 tcggcatatc gggaatgaat ccgacgcctg cgttgtcgct caccctgacg cgacgcacca
     6421 gccggaggcc atgctgaccg aacacctt
//
LOCUS       DASRJA010000008         4011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2802683,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000008 DASRJA010000000
VERSION     DASRJA010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2802683"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VFC01_00360"
     CDS             complement(<1..906)
                     /locus_tag="VFC01_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZN78130.1"
                     /translation="MIQEHRYDVVIVGAGGAGMRAAVEAGPRARTAVLTKLYPTRSHT
                     GAAQGGMCAALANVEEDNWEWHTFDTVKGGDYLADQDAVEIMCKEAIDAVLDLEKMGM
                     PFNRTPEGRIDQRRFGGHTRDHGKAPVRRACYAADRTGHMILQTLYQNCVKHDVQFFN
                     EFYALDLALTETAAGPVATGVVAYELATGDIHVFHAKAIVLATGGSGRMYKTTSNAHT
                     LTGDGLGIVFRKGLPLEDMEFHQFHPTGLAGLGILISEAVRGEGGILLNDEGERFMER
                     YAPTIKDLAPRDIVARSMVREVLEGR"
     gene            complement(920..1414)
                     /locus_tag="VFC01_00365"
     CDS             complement(920..1414)
                     /locus_tag="VFC01_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HZN78131.1"
                     /translation="MTGPSGGAAGGSWTPHHREGRIAPVQEREHDRPASLDHPRAPRR
                     PRGIPYFEKYAWLFMRFSGVALVFLALGHLFIGLIWDGGVYRIDFNYVAERWASPFWQ
                     IWDMALLWLAMVHGANGMRTIIGDYARKNITKFYLNSLLLLATGFTLVLGTYVLVTFD
                     ASIS"
     gene            complement(1411..1830)
                     /gene="sdhC"
                     /locus_tag="VFC01_00370"
     CDS             complement(1411..1830)
                     /gene="sdhC"
                     /locus_tag="VFC01_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HZN78132.1"
                     /translation="MSTATPESPAVPAPRSPRRTLYRGDPGMWSWVLHRITGATIFFF
                     LLVHVLDTALVRVSPEVYNEVINTYKTPIVGLMEVGLVAAVLYHALNGIRIILIDFWQ
                     HGPKYQRLMLWIVIGVFLAVMIPALGVLGMHMAERFL"
     gene            2070..2495
                     /locus_tag="VFC01_00375"
     CDS             2070..2495
                     /locus_tag="VFC01_00375"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HZN78133.1"
                     /translation="MTSDIDWKMLRHKATEVAAHAYAPYSGFAVGAAALVDDHRMVAG
                     CNVENVSYGLGLCAECAVVCALHSSGGGRLIALSCVDAAGDVLMPCGRCRQVLMEHGG
                     PGLLIDHPLGPRPLSQLLPDAFGPQDLARRNEPAHEETP"
     gene            2578..3063
                     /locus_tag="VFC01_00380"
     CDS             2578..3063
                     /locus_tag="VFC01_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HZN78134.1"
                     /translation="MESRAKALGHAIHPMLIPFPLGLLATAVVFDIVYLITDRPGFAT
                     AAAYMIAAGIIGGLLAAPFGWIDWFKIPSGTRAKSVGLIHGLGNIVVVVLFAVSWLVR
                     LGNGWEPTAWALVCSFVAVALAVVTAWMGGELVERMGVSIDDGAGVDAPSSLSHRHVA
                     A"
     gene            3101..>4011
                     /locus_tag="VFC01_00385"
     CDS             3101..>4011
                     /locus_tag="VFC01_00385"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HZN78135.1"
                     /translation="MTAFAFDAPTVIRTKRDGGVLSDAAIDWVIDGYTHGRIADEQMS
                     ALLMAIFLRGMTGAEIARWTAAMVDSGERLDFTDLRRDGKPLALVDKHSTGGVGDKIT
                     IPLVPVVMACGGAVPQAAGRGLGHTGGTLDKLESIPGFTAEISKAQIRQQLCDLGAAI
                     FAAGELAPADRKIYALRDVTGTTESLPLIASSVMSKKIAEGTRALVLDSKVGSGAFLA
                     TEAESRELARTMVELGTAEGLVTRALLTDMETPLGRTVGNAVEVEESLEVLAGGGPAD
                     VVELTLALAVEMLDAAGLDGIDPAETL"
BASE COUNT          688 a         1275 c         1370 g          678 t
ORIGIN      
        1 gcggccctcg agcacctcgc gcaccatcga gcgggcgacg atgtcgcggg gcgccaagtc
       61 ctttatggtg ggggcgtagc gctccatgaa ccgctcgccc tcgtcgttga gcaggatgcc
      121 gccctcgccg cgcaccgcct ctgagatcag aatgccaagg cccgcaaggc ccgtcggatg
      181 gaactggtga aactccatgt cctccaacgg aagtcccttg cggaacacga tgccaaggcc
      241 atcgccggtc agcgtgtgcg cgttggaggt cgtcttgtac atccggccgg agccaccggt
      301 ggcgagcacg atcgccttgg cgtggaagac gtgaatgtcg ccggtcgcca gttcataggc
      361 gacgacgccc gtcgcgacgg ggccagcggc cgtctccgtc agcgccaggt ccagcgcgta
      421 gaactcgttg aagaactgga cgtcgtgttt gacgcagttt tggtacagcg tctgcaggat
      481 catgtggccg gtgcggtcgg cggcgtaaca ggcccgccgc actggcgcct tgccgtggtc
      541 gcgggtgtgc ccgccgaacc ggcgctggtc gatgcggccc tcgggggtgc ggttgaacgg
      601 catccccatc ttctcgaggt cgagcaccgc gtcgatggct tccttgcaca tgatctcgac
      661 cgcgtcctgg tcggcgagat agtcgccgcc cttaacggtg tcgaaggtgt gccactccca
      721 gttgtcctct tcgacattgg ccagcgccgc gcacatgccg ccctgcgccg cgccggtgtg
      781 gctgcgggtc gggtagagct tggtcaacac cgcggtgcgc gcgcggggac cggcctcgac
      841 cgcggcgcgc atgcccgcgc cgccggcgcc gacgattacg acgtcgtagc gatgctcttg
      901 gatcattgga acctccccgt cacgagatgc tcgcgtcgaa ggtgaccagg acatacgtcc
      961 ccaacaccaa cgtgaacccc gtcgccagca acagcaggga gttcagatag aacttcgtga
     1021 tgttcttgcg cgcgtagtcg ccgatgatgg tgcgcatgcc gttggcgccg tgcaccatcg
     1081 ccagccagag caacgccata tcccagatct gccagaacgg tgaggcccac cgctcggcga
     1141 cgtagttgaa gtcgatgcgg tagacgccgc cgtcccagat caacccgatg aacagatgcc
     1201 cgagcgccag gaataccagc gccacgccgg aaaaccgcat gaacagccag gcgtacttct
     1261 cgaagtacgg gatgcccctc gggcggcggg gtgcgcgcgg atgatccaac gaggccgggc
     1321 ggtcgtgttc tctttcctgc accggcgcaa tccggccctc gcggtggtgc ggtgtccagg
     1381 atcctcccgc cgccccgccc gatggcccgg tcataggaac cgctccgcca tgtgcatgcc
     1441 gagcacgccc agcgcgggga tcatgacggc gagaaagacg ccgatgacga tccacagcat
     1501 cagccgctgg tacttcgggc cgtgctgcca gaagtcgatc aggatgatcc ggatgccgtt
     1561 cagcgcgtga tagagcaccg cggcgaccag tccgacctcc atcaggccga cgatcggcgt
     1621 cttgtaggtg ttgatgacct cgttgtagac ctccggactg acccgcacga gggcggtgtc
     1681 cagtacgtgc actaacagga agaagaagat ggtcgcaccc gtgatgcggt gcaagaccca
     1741 ggaccacatc ccgggatcac cgcgatagag ggtgcgccga ggcgatcgcg gagccggcac
     1801 cgccggactc tcgggtgtcg cagtactcat ctggcctcca acgccatcgt ggacgcttgg
     1861 gctcggagcc gccacggcgg cgatatcggc cgtggctgag aacctgccca aacctcgggg
     1921 cgactctaaa cccacttgta gtgctaaacg aactacggtt tagccgtcag cacaccgaaa
     1981 aacgtgaaag ttagggttac ctattccgcg aggcggacca gcgagccagg ccttcggaat
     2041 gcattcagat tcggcccgat gaggttgaca tgacatccga tatcgactgg aagatgttgc
     2101 ggcacaaggc aactgaggtc gccgcgcacg cctacgcacc atattcgggg ttcgccgtcg
     2161 gggccgccgc gctggtcgac gatcaccgga tggtggccgg ctgcaatgtg gaaaatgtct
     2221 catatggcct tggtctctgt gccgagtgcg ctgtggtgtg cgccttgcac tccagcgggg
     2281 gcggcaggct gatcgcgctg tcgtgtgtgg acgcggcggg cgacgtgttg atgccctgcg
     2341 gacgctgtcg gcaagtgttg atggaacacg gcgggccagg cctgctcatc gaccatccgc
     2401 tgggtccgcg gccgctgagc caactgcttc ccgacgcgtt cggcccgcag gatctggcgc
     2461 gccgcaacga accggcgcat gaagaaacac cgtgatcggc ttcacgtttg ccctgtttga
     2521 ggtggcagga gtcgggtaac tcggttgccg tcacgcattt gcataaggag gcggcaaatg
     2581 gagagtcggg caaaggcgtt gggtcacgca atacacccca tgctgatccc gttcccgctg
     2641 gggctgctgg ccaccgcggt cgtattcgac atcgtgtacc tcatcaccga ccggcccggt
     2701 ttcgcgaccg cggcggccta catgatcgcg gcggggatca tcggcgggtt gctcgcagcg
     2761 cccttcggct ggatcgactg gttcaaaatc ccttcaggca cccgggccaa gagcgtcggg
     2821 ctgatacacg gcctcggcaa catcgtggtg gtcgtgctgt tcgcggtgag ctggctggtc
     2881 cggctcggca acggctggga gccgaccgca tgggcgctgg tgtgcagctt cgtcgccgtg
     2941 gcgctggccg tggtgaccgc atggatgggc ggcgagctgg tggagcgcat gggtgtcagc
     3001 atcgacgacg gcgcaggtgt cgacgcgccg agctcgttga gccaccggca cgtggccgcg
     3061 tagcagcatc gcatagaccg ccaccggtaa cgtaacggcg gtgacggcgt tcgcatttga
     3121 cgcgccgacg gtcatccgga ccaagcgcga cggtggagtc ttgtcggatg cggccatcga
     3181 ctgggtgatc gacggctata cccatggccg gatcgccgac gagcagatgt cggcgttatt
     3241 gatggcgatc ttcctgcggg gcatgaccgg cgccgaaatc gcccgctgga cggcggccat
     3301 ggtggactcc ggtgagcggc tggactttac ggatctgcgg cgcgacggca agccacttgc
     3361 gttggtggac aagcattcca ccggcggcgt cggcgacaag atcaccattc cgctggtgcc
     3421 cgtcgtgatg gcctgcgggg gagctgtgcc gcaggcggcg ggacgcggcc tcgggcacac
     3481 cggcgggacc ctcgacaagc tggagtccat cccgggtttc accgccgaga tctccaaagc
     3541 ccaaatccgg caacaacttt gcgaccttgg ggcggcgatc ttcgcggccg gcgagttggc
     3601 ccccgccgat cggaagatct atgcgctgcg cgacgtcacc ggcaccaccg agtcactgcc
     3661 gctgatcgcc agctccgtga tgagcaagaa gatcgccgag ggcacccggg cgctggtgct
     3721 cgacagcaag gtcggcagcg gagcgttcct ggcgaccgag gcggagtccc gcgaactggc
     3781 ccgcacgatg gtggagctgg gcaccgccga gggattggtg acccgcgcac tgctgaccga
     3841 catggagaca ccgctgggcc gcaccgtcgg caacgccgtc gaggtcgagg aatcgctgga
     3901 ggtgctggcc ggcggaggac ctgccgatgt ggtggagctg acgctggcgc tggccgtcga
     3961 gatgctcgac gccgccgggc tcgacgggat cgaccccgcc gagacgctga a
//
LOCUS       DASRJA010000009        20337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2247514,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000009 DASRJA010000000
VERSION     DASRJA010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20337
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2247514"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /locus_tag="VFC01_00390"
     CDS             complement(<1..1627)
                     /locus_tag="VFC01_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN78136.1"
                     /translation="MTQTEVAEDPPLSHLRLTARLNTSALDARRGVVRLHPEAIAALG
                     IREWDAVSLTGSRTTAAVAGVAPAGTPAGTALLDDVTLSNAGLREDTTVIVSAVTVYG
                     AKSVTVSGSSLATQSISSATLRQALLGKVMTVGDTVSLLPRELGPDIPTSTATAALAS
                     SVGITWTSELLTVTGVDPAGPVSVQPNSVVSWGDGVTATGPTTAGQHTVSTPKQTVNI
                     DDLKGSHAQASRLTEWLKLSLDQPQLLETLGATANLGVLVSGPAGVGKAALVRAVCAD
                     RRLVELDGPEVGALRAEDRLKSVSSAVATVRDGGGVLLITDIDALLPATAEPVATLIL
                     TELRNAVATRGVAFIATTAVPDGLDPRLRAPDLCDRELGLSLPDGAVRKQLLEVLLRD
                     VPSSELNLDEIAERTPGFVVADLAALVREGALRAAARASADGKPPVLTQDDMIGATTV
                     IRPLSRSATEEVSVGSVTLDDVGDMAETKQALTEAVLWPLQHPDTFQRLGVAPPRGVL
                     HYGPPGCGKTFVVRALASSGRLSVHAVKGAELMD"
     gene            complement(1629..2486)
                     /gene="pssA"
                     /locus_tag="VFC01_00395"
     CDS             complement(1629..2486)
                     /gene="pssA"
                     /locus_tag="VFC01_00395"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HZN78137.1"
                     /translation="MIRPRVKPSVISLRILPSAMTVAAICLGLSAVKMALDNRPTEAM
                     AFLAVAAILDALDGRIARILKVASRMGEEIDSLADAVNFGVAPAFIVYGTLLSHSRVG
                     WIVVLLYAVCIVLRLARFNAMLDVDKPAYEKEYFVGMPAPAGAIGAIGPLAAKMQFGD
                     GWWTSEVAVVIWMIGVSLLVVSTLPMRKIHTFSVSPNMVPTLLLGVVVLVAASIFYGY
                     LTILAIIVAYVIHIPFAIRTRRFLAAHPEVWDDKPRQQRAARRAIRRAAQPQRRRSNL
                     RLGLRRPGR"
     gene            complement(2483..3181)
                     /locus_tag="VFC01_00400"
     CDS             complement(2483..3181)
                     /locus_tag="VFC01_00400"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HZN78138.1"
                     /translation="MARRPDLKSGPARLVALVRTTIPPMHPAGLPFVGASLAVAAVGR
                     KNRWLRRAGLASAAANAAFFRHPPRVAPTRPGLVVAPADGLICLIEKATPPAELGLPA
                     TPLPRISIFLSIFDAHVQRAPIGGEVVAVEHRPGLFHSAELEAASEDNERNSVVIRSP
                     DGVEVIAVQIAGLVARRIVCEAKVGDKLTIGDTYGLIRYGSRLDTYLPAGSNVLVTTG
                     QRSLAGETVLAELP"
     gene            complement(3208..4401)
                     /gene="glp"
                     /locus_tag="VFC01_00405"
     CDS             complement(3208..4401)
                     /gene="glp"
                     /locus_tag="VFC01_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZN78139.1"
                     /translation="MRTVDEHRRVVAGLITGQPPVTLPLAETLGLALADDVVAPLSLP
                     GFDNSAMDGYAVVDADVAAATPEQPVLLPVAEDIPAGRTDPLTLKPGTAHRIMTGAPV
                     PAGATAVVPVEATDGATDTVSIRVSAKDGQHIRRAGEDVTAGTTVLRAGQVLTPAALG
                     LTAALGLGELKVIPRQRVLVMSTGSELVAPGTPLQPGQIYESNAVMLAAAVRDAGGHV
                     VAAPMTGDDVDVFRDALHRHAGGADLIITTGGVSAGAFEVVKDSLGGEVDFVKVAMQP
                     GMPQGAGSVNGTPIITLPGNPVSALVSFEVFIRAPLRTAMGLPHPDRPRRTAVLAEDL
                     TSPRGKRQFRRGVLDRKAGTVTSYGPPASHHLRWLASANCLLEIDEDVAELAAGSPVQ
                     VWDLA"
     gene            complement(4401..5312)
                     /locus_tag="VFC01_00410"
     CDS             complement(4401..5312)
                     /locus_tag="VFC01_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN78140.1"
                     /translation="MSSKWTAADVPDQTGRVAIVTGANSGLGYDTAAVLADKGAHVVL
                     AVRNLDKGNEAADRIKSTSPNADVALQQLDLTSLDSVRKAADELRATHPRIDLLINNA
                     GVMYVPTRESTKDGFEMQFGTNHLGHFALTGQLLDNILPVEGSRVVTISSVGHRLMAR
                     IHFDDLQLERKYNRVEAYGQSKLANLLFTYELQRRLNAKGSPTIAAAAHPGFSDTELM
                     RHLPGFIPDFAWRPFTQPPDMGALPTLRAATDPGVQGGQYYGPDGIGETRGHPKVVGS
                     SAQSQNEDLQRRLWTVSEELTGVTYPV"
     gene            5367..5846
                     /locus_tag="VFC01_00415"
     CDS             5367..5846
                     /locus_tag="VFC01_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN78141.1"
                     /translation="MGKRIVATVLGSLIGAVVLFLGLLLAGLRWQVTPVVDFVRRMNK
                     SVSNPRVMRTAGTTQTQTSVIEHVGRTSGRTYQTPIDVIPTRTGLLIALPYGARADWT
                     RNVLAAGAATVVTQGERVTVDRPTIVAAADVASLIPKRTIRMLRLFGVRECLHLEKS"
     gene            5843..6325
                     /locus_tag="VFC01_00420"
     CDS             5843..6325
                     /locus_tag="VFC01_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZN78142.1"
                     /translation="MNLVEPANPPSRKAPLVWAIGAAIPWLMLAAGQAVWFAIDPRPG
                     WLHALAAAATVGGIVLFVAVVPLWRYRVHRWDIDPKAVYTRTGWLVQERRIAPISRVQ
                     TVDTERGPLDQLFGLANVTVTTASSAGAVRIVALDSDVADRVVAQLTDIAALGGEDAT
                     "
     gene            6399..7874
                     /locus_tag="VFC01_00425"
     CDS             6399..7874
                     /locus_tag="VFC01_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZN78143.1"
                     /translation="MSTDAGQVWQRLSPRMLLVHPVHEVLRQIPLLIGSVVLGSATGN
                     PLWGLVGLALIVGYGAARWFTTTYRIGPDEVQLRTGVLQRKVLSVPRNRIRSVSTDAR
                     LLHRLLGLTVLRLSTGQEARGDAAFALDAVEAEQVPRLRATLLADSLAPVEQEASGRE
                     LARWQPSWLRYSPLSFTGLAMIAAAVGLVYQAGAGAALQDSRLARSGLDAAERFGVAA
                     SVILAGVVVLVASVVLSVLRSLVTYGNLVLRRDAEVLHLEHGLLRRRERTFDMRRLRG
                     GTLREPLLVRLFGGARLDAVMTGVDGAGEASLLLPPCPRATAEEVLSELIATTDADER
                     STKYFDPVTGPLRAHGAAATRRRWTRALTLPPVLLIAMVAVPAPGWLWPVWAVLTVCC
                     ALLAVDRSRALGHRVVDDEWLVARAGSLDRRRDCIAAAGIIGWTVRQTLLQRRAGVAT
                     LIAATAAGVKRYQVIDVPADLAWTIAAATSPWVADNQWAKP"
     gene            complement(7864..7998)
                     /locus_tag="VFC01_00430"
     CDS             complement(7864..7998)
                     /locus_tag="VFC01_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78144.1"
                     /translation="MAGPSRVTDPDGKVWTVRRCWFPWRRALSTKIQRGELTAEPVKA
                     "
     gene            8031..8507
                     /locus_tag="VFC01_00435"
     CDS             8031..8507
                     /locus_tag="VFC01_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN78145.1"
                     /translation="MITGLAHSGVCVPDCEAAVAYYRDVLGFTVLSPPYVMDGDAIRA
                     DMGELLTDPAMKAAIVGFGGDGDRVLEVIEYLRADGSDARRERLADPGLSHVGLICDD
                     VDETRKALEDKGVHFLVDGIADIAGVRTTWFADPWGVIFILVEKSKPERPYFAQFG"
     gene            8550..9365
                     /locus_tag="VFC01_00440"
     CDS             8550..9365
                     /locus_tag="VFC01_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HZN78146.1"
                     /translation="MAIGGVLFDIDGVLVTSWKPIEGAAETLQMMARNQIACSYLTNT
                     TTRTRVQIAELLTEAGMQVRADEVITAAVLTAEYVRNRYPDARCFLVNSGQIAEDMPG
                     IDIVYSSEFSGPKAPDTPDVVLLGGAGPEYSHLTLSWVYDWMAQGVPVVAMHRSTAWT
                     TTDGLRVDTGMYLIGMEETSGRKATAVGKPAPEGFLAAASRLGVDPDEMYMVGDDLNN
                     DVLAAQVVGMTGVLVRTGKFRQDTLDRWAADEFAMQPNHVIDSVADLPALLAL"
     gene            9477..9953
                     /locus_tag="VFC01_00445"
     CDS             9477..9953
                     /locus_tag="VFC01_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN78147.1"
                     /translation="MTEESMSVTATINAPAQKVFDVLVDPSTHEAIDGTGWVRQSLDG
                     KRITETGEVFRMAMYHENHPNKNYEMANRVEVFEPPHAVAWQPGQGPDDANLDFGGWI
                     WRYDLKPVGSAQTEVTLTYDWSRVPPSTREHIGFPPFEAQHLENSLKHLAELSQSR"
     gene            complement(9939..11837)
                     /locus_tag="VFC01_00450"
     CDS             complement(9939..11837)
                     /locus_tag="VFC01_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZN78148.1"
                     /translation="MNDSLGLSVGMTNLGAARSGWPPALGRSILTLYPDRAPEVGAPE
                     QNSPPGEHGMVLAGFVERVGDPIPLVAADGSTHRPGRLLVEALESLMRIPESGRPPHD
                     IAIAVPAHWDSRAKASFHQDLSAKQRLFPAGPPPLISDATASLSALQHHRGLPSRGVV
                     ALLDFGGTGTSITLCDAARGWAPIGETMRCTDFGGQQIDQSILARILSGIAEAANIDP
                     DSTGAVLSLSRLRDQCRKAKETLSADTVATVTAELPAFRSNVRLTRAELEDLVAKPLG
                     GVIAALEDTLDRNRIPPANVAAIATVGGGANIPLVTQRLSQHLRIPVVTTPRPELAPA
                     TGAALIAARGPAAPATAMAPAARVAAGVAAAGAAADSPATETFRALAWSDDVAADEPL
                     PYSGETDDADRETADPVRPQLEFIRDPDAVDEEAPFAWYRRPQLLLGVAAAAVLLTGG
                     GLTYSLTSSSGSPAPAPAPSAVPVVVQPQAPPPAAGTPPPAEQAPAPVIVRQAPAAPR
                     VQPRPASPPAPRRAGQAPVAPPPAAPAPSTSASPPTTTSAPTTTTSTSATSTSPTSTE
                     PTSSQPPSSSEPPTTTQEQTAAQVPSTTQAPVATPPPMTTQAPAATEAPVNTPEITFP
                     SLPSLPGL"
     gene            12018..13067
                     /locus_tag="VFC01_00455"
     CDS             12018..13067
                     /locus_tag="VFC01_00455"
                     /inference="COORDINATES: protein motif:HMM:NF038175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IniB N-terminal domain-containing protein"
                     /protein_id="HZN78149.1"
                     /translation="MANLIDYVMDLFRDEGQAQSFVDDPQGALAAIGLPNVTPEQIQA
                     VAAMAAPGGAMAAPAGTGMTAGDPMGTLRQAVASHHGMPAPAGGGGGAPHPAEHVQQA
                     AAFAPTSHAAGLAGSTQGDVTHATFAPQSNDLSNRETNVDSHNTTGDFMSPHTDGVQQ
                     GGGGFNLGMDFGDITLGDKNTASGDGAVAMSGDNSGSIVSGDGAVLGDGNTVLNGNID
                     AGDGSPITFGDGNEVDATSMKAGGDMISGNEGPVLNGVDTGGGSLGLDNSSTVGGDQN
                     NTDISGEIGSIDGDLDTSHHDDKSVDASDHSVTTVDSHDVSDSYNDQSKQETTAGFDG
                     GDVGGDFSLGGILPF"
     gene            13105..14988
                     /locus_tag="VFC01_00460"
     CDS             13105..14988
                     /locus_tag="VFC01_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin-like GTPase family protein"
                     /protein_id="HZN78150.1"
                     /translation="MTQPANDPRNVKLLVELIDHTTKIAELNGRGDLSERLARARKRI
                     SDPRIRVIVAGQLKQGKSQLVNSLLNLNVCKVGDDESTAVATVVSYSEKPWARLVVEQ
                     PDGSEPEIIEIGVGELKNDLRRAPQAGGREVIRVEVGAPSPMLKSGLALVDTPGVGGY
                     GQPHLAATLGLLPDADAVLMVSDLSQEFTEPEMTFMRQAFEMCPVATIVGTKTDIYPH
                     WRDIVYANTTHLQRARIATPIIPVSSILRSHACATNDNELNEESNFPAVVRFLADHVL
                     SRENDRIRDQVLAEIASAAEHLAFSLNTEFESINDPDARQKLTEDLERRKREAQDALA
                     QTALWQQVLNDGIADLTGDVDHDLRSRFRSITQHTEKVLDGCDPTVCWVQLATELEDT
                     VATAVGDNFVWAYQRAAALADEVGQTFAEAGLEAIDAPVINASEIGASFGGVNGVGRL
                     DAKPVGKGQKVLTGVRGSYGGMMMFGMLTSLAGLPMMNPLAVGAGLLLGRKAYKEDRD
                     NRLNRARAEAKMNMRRFIDDVSFVVTKESRDRLKLVQRQLRDHYRDIANQANRSLNES
                     LQATIASARMKEAESNTRIKELERQLKIVRAVTDHTTKLSGGSAPKTPTSPQAAPAVA
                     AVG"
     gene            15004..15354
                     /locus_tag="VFC01_00465"
     CDS             15004..15354
                     /locus_tag="VFC01_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78151.1"
                     /translation="MSIGDFIDDPFGAVAKAGLDAFAQGGISGENADVVGGFEVPDVR
                     SMTPFFGDDKSDPYQLGGVGEQPSEPYVDNSGPQIIHPDPMPDTAPEPSTAADDAAGY
                     GAAGEVDSGTYDIF"
     gene            15388..15996
                     /locus_tag="VFC01_00470"
     CDS             15388..15996
                     /locus_tag="VFC01_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78152.1"
                     /translation="MSVSDFINDPFGAVAKAGVDGMPDEVKETVHDVAQQITNPAMPD
                     SGALTEDFGNADGSFGDAGTLGKVSAPEMHTLGENTFGGIDIADQSIIHPDPYADPEP
                     PHYDVEVGPATIIDPEEGTAGEQAQYSGADGYIYTSDYGGSAAEEAAGSGVSAYGPDT
                     DPNYSISPVGEDGPAAADYGPGATGELAGAGGEVTTEDDSFF"
     gene            complement(16008..17690)
                     /locus_tag="VFC01_00475"
     CDS             complement(16008..17690)
                     /locus_tag="VFC01_00475"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN78153.1"
                     /translation="MRAYAAPDTQALRGWQRRALVRYLGAKPRDFLAVATPGAGKTAF
                     ALRIAGELLADGTVERITVVVPTEHLKTQWALAAARVGIALDPKFTNSSAQTSSEYHG
                     VVVTYAQVASHPTRHRVRIENYRTLVVFDEIHHGGDAKSWGEAIREAFADATRRLALT
                     GTPFRSDDSAIPFVTYEPDGDGLMRSRADHTYGYAEALGDGVVRPVVFLAYSGEARWR
                     DSAGEEHAARLGEPLTAEQTARAWRTALNPAGEWIPAVLQAADKRLNQLREGGMPDAG
                     AMVIASDQTAARAYAEVLTKLTGQAPTVVLSDDPGSSARIADYAAARSPWLVAVRMVS
                     EGVDVPRLAVGVYATSASTPLFFAQAIGRFVRSRQPGETASIFLPSVPNLLLLASEME
                     AQRNHVLGKPHRESQRDDEYVERRKSEPSELDNGFESLGADAELDQVIFDGSSFGTAT
                     PAGSEEEADYLGIPGLLDADQMRDLLRRRQDEQLSKRTASGEVPRPSMHGQLRDLRRE
                     LNTLVSIAHHRTGKPHGWIHNELRRLCGGPPIAAATTDQLKARIEAVRTLQA"
     gene            complement(17762..18259)
                     /locus_tag="VFC01_00480"
     CDS             complement(17762..18259)
                     /locus_tag="VFC01_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN78154.1"
                     /translation="MSLIVPPYPPARYTKDEPEVSAWLRRGTEPPDYDSFGLVKYHYL
                     ANQQATDGDYGLYRVEIAPKGGGPGPHFHRAMSEAFFVLSGTVKLYDGTDWIDGHPND
                     FLYVPPGGIHGFRNEAEEPTSILMLFAPGAPREHYFEGMAQLADMSDDERREWFIAND
                     NFFVE"
     gene            complement(18280..19188)
                     /locus_tag="VFC01_00485"
     CDS             complement(18280..19188)
                     /locus_tag="VFC01_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN78155.1"
                     /translation="MSNWTTADIPDQTGRTAVITGANTGLGYETATALAAKGAHVVLA
                     VRNLEKGKAAADLITARTPGASVALQELDLTSLESVRNAADELRSEHDQIDLLINNAG
                     VMMTPKSHTKDGFELQFGTNHLGHFALTNLLLDRVLAVPGSRIVTVSSQGHRFARGIR
                     FDDLQWDRDYGRVRAYGQAKLANLLFTYELQRRLHGTHTIAVAAHPGGSNTELMRNLP
                     VAIRGLGTLLAPLFQGPDMGALPTLRAATDPGVLGGQYYGPDGFGEQRGYPKVVASSV
                     ASHDVDAQRRLWAVSEELTSVVSPVG"
     gene            19287..19850
                     /locus_tag="VFC01_00490"
     CDS             19287..19850
                     /locus_tag="VFC01_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN78156.1"
                     /translation="MAELARPLRADAARNRARVLEVAYETFAAEGLGVPIDEIARRAA
                     VGAGTVYRHFPTKEDLYRAVIEDRINHIVDDGRALLADADPGEALFTFLRSMVLQWGA
                     TDRGLAEALAGLGIDVKDAAPEAEEAFLGLLGELLRASQKAGTVRRDVEVPDVKALMV
                     GAQAIQTYNADAAERLTEVVLDGLRAG"
     gene            20033..>20337
                     /locus_tag="VFC01_00495"
     CDS             20033..>20337
                     /locus_tag="VFC01_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HZN78157.1"
                     /translation="MAKTIAYDEEARRGLERGLNSLADAVKVTLGPKGRNVVLEKKWG
                     APTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREG
                     "
BASE COUNT         3391 a         6520 c         7095 g         3331 t
ORIGIN      
        1 tgtccatcag ctcggcgccc ttgaccgcgt gcaccgacag ccgtccggaa ctggccagtg
       61 cacgcaccac gaatgtcttg ccgcagccgg gcgggccgta gtgcaaaacg ccgcgcggtg
      121 gggcgacgcc gagccgctgg aaggtgtcgg gatgctgcag cggccagagc accgcctcgg
      181 tgagcgcctg tttggtctcg gccatgtcgc cgacgtcgtc gagcgtcacc gaaccgaccg
      241 aaacctcttc ggtggccgag cgcgacagcg gccggataac ggtggtcgca ccgatcatgt
      301 cgtcctgcgt gagaacgggc ggcttgccgt cggcgctcgc ccgcgccgcc gctcgcaacg
      361 caccctcgcg caccaacgct gccaagtcag cgaccacgaa acccggtgtg cgctccgcga
      421 tttcgtcgag attgagctcc gaggacggga cgtcgcgcaa cagcacctcg agcagctgct
      481 tgcggacggc tccgtcgggc aggctcagcc cgagctcacg atcacacaag tcgggtgccc
      541 gcagtcgcgg gtcgagaccg tccggcaccg ccgtggtggc aatgaaggcg acgccgcgcg
      601 tggcgaccgc gttgcgcagt tcggtgagaa tcaatgtcgc gacgggttcg gcggtcgccg
      661 gcaggagcgc gtcgatgtcg gtgataagta gtacgccgcc gccatcgcgc acggtggcca
      721 cggccgacga gacactcttg aggcggtctt cggcgcgcaa cgcgccaact tccggaccgt
      781 cgagctcgac gaggcgtcgg tcggcacata cggcgcgcac cagcgcggcc ttgccgacgc
      841 ccgcggggcc agacaccagc acaccgagat tggctgtggc gccgagtgtt tcgagcaact
      901 gcggctgatc gagggagagc ttgagccatt cggtcagccg cgaggcctga gcgtgcgaac
      961 ccttgaggtc atcgatgttc acggtctgct tcggcgtcga caccgtgtgc tgacctgcgg
     1021 tggtcggccc ggtcgcggtc acgccgtcgc cccaactcac caccgaattc ggttgcacgc
     1081 taaccggtcc cgccgggtcg acacccgtca ccgtcaacaa ttccgacgtc caggtgatgc
     1141 cgaccgacga tgccagcgcc gcggtggccg tcgacgtcgg gatgtcgggg ccgagctcgc
     1201 gcggcagcag cgacaccgtg tcgcccaccg tcatcacctt gcccagcagg gcctgacgca
     1261 gcgtcgccga cgagatcgac tgggtggcaa ggctcgaacc gctcaccgtc accgatttgg
     1321 cgccgtaaac ggtcaccgcc gagacgatca cggtggtgtc ttcgcgtagc ccggcgttgg
     1381 acagcgtcac gtcgtcaagc agcgcggtgc cggccggcgt ccccgcaggt gcgactcctg
     1441 ccaccgcggc cgtagtgcgc gaacccgtca gcgacaccgc gtcccactcc cgaatgccaa
     1501 gggctgcaat tgcttccgga tgcagccgga ccacaccacg ccgcgcgtca agcgccgacg
     1561 tattcagccg tgcggtcagc cgcaggtggc tcagcggcgg atcctccgct acttcggtct
     1621 gcgtcatctc agcggcccgg cctgcgcagt ccgagtcgca ggttggaccg gcggcgttgt
     1681 ggctgtgccg cgcgtcggat cgcccggcgc gccgcgcgtt gttgtcgtgg tttgtcgtcc
     1741 cacacttccg gatgcgcggc caagaagcgt ctggtgcgga tcgcgaacgg gatgtggatg
     1801 acgtaagcga cgatgatcgc caggatcgtg aggtagccat agaagattga cgccgcaacg
     1861 agaacgacca cgccaagcag gagcgtgggc accatgttcg gcgacaccga gaaggtgtgg
     1921 atcttgcgca tggggagggt gctgacgacc agcagcgaaa cgccgatcat ccagatcacc
     1981 acggccactt ccgatgtcca ccagccgtcg ccgaactgca tcttggcggc aagcgggccg
     2041 atcgcaccga tcgcgcccgc cggggcgggc atgccgacga agtactcctt ctcgtaggcg
     2101 ggtttgtcga cgtcgagcat cgcgttgaag cgggccagcc gcaacacgat gcacaccgcg
     2161 tagagcagta ccacgatcca gccgaccctc gagtgcgaga gcagcgtgcc gtagacgatg
     2221 aatgccggtg ccacaccgaa attcacggcg tcggccaggg agtcgatctc ctcgcccatc
     2281 cgcgaggcca ctttgaggat gcgggcgatg cgaccgtcga gcgcgtcgag gatcgcggcg
     2341 acggccagaa acgccatcgc ttcggtcggg cggttgtcca acgccatctt gaccgcgctc
     2401 agccccaggc agatggccgc caccgtcatg gcgctgggca ggatgcgcag gctgatgacc
     2461 gacggcttga cgcggggtct gatcacggca gctcggccaa aacggtttcg ccggccagcg
     2521 accgctgtcc ggtagtgacc aagacgttgg aacccgcagg caggtaggtg tccagccgcg
     2581 agccgtaccg aatgaggccg tacgtgtcgc cgatcgtcag cttgtcgccg accttcgcct
     2641 cgcacacgat gcggcgcgcc accaggccgg cgatctgtac cgcgatcacc tcgacgccgt
     2701 cgggactgcg gatcaccacg ctgttgcgtt cgttgtcctc actcgcggcc tccaactcgg
     2761 ccgagtgaaa cagcccgggg cggtgctcga cagccacgac ctcgccgccg atcggcgccc
     2821 gctgcacgtg cgcatcgaag atcgacagga aaatgctgat gcgcggcaac ggcgttgcgg
     2881 gcagccccag ttcggcaggc ggcgtggctt tctcgatgag gcagatgagc ccgtcggccg
     2941 gcgcgacgac aaggccgggg cgtgtgggtg cgacccgcgg cggatgccgg aagaacgccg
     3001 cgttggcggc ggctgacgcc agacccgctc tgcgcagcca gcggttcttt cgtccgaccg
     3061 ccgctaccgc caggcttgcg ccgacgaacg gcaggcccgc agggtgcatc ggcggaatag
     3121 tggtgcggac cagcgccacg agccgcgcgg ggccggattt gaggtcgggg cgtctggcca
     3181 tcgtgcagcg attctacggg cgggctgtta cgccaggtcc catacctgca caggggaccc
     3241 cgcggccagc tcggcgacgt cctcgtcgat ctccagcagg cagttggccg aggccagcca
     3301 gcgcagatgg tgggatgccg gcgggccgta gctggtgacg gtccccgcct tgcgatcgag
     3361 cacgccgcgt cggaattgac gctttccgcg cggcgaggtc agatcctcgg cgaggacggc
     3421 cgtgcgccgc ggtcggtccg gatgtggcag ccccatcgcg gtgcgcagcg gcgcgcggat
     3481 gaacacctcg aacgacacca gcgcactgac cgggttgccg ggcagcgtga tgatcggcgt
     3541 gccgttcacg gagcctgcac cctggggcat gccgggttgc atggccacct tgacgaagtc
     3601 gacctcgccg ccgagcgaat ccttgaccac ttcaaaggct cccgcgctga cgccgcccgt
     3661 ggtgatgatc agatcggcgc cgccggcgtg gcggtgcaga gcgtcgcgga atacgtcgac
     3721 gtcgtcgccc gtcatcggtg ccgcgaccac gtggccaccg gcgtcgcgca cggccgcggc
     3781 gagcatcacc gcgttggact cgtagatctg gcctggctgc agcggagtgc ccggcgccac
     3841 cagctccgaa ccggttgaca tcaccaaaac ccgttgcctt ggaatgactt tcagctcgcc
     3901 caagcccagg gcggcggtca ggcccagcgc cgcgggcgtg aggacctggc ccgcccgcag
     3961 cacagtcgtg cccgccgtga catcttcacc ggcgcggcgg atgtgctggc cgtccttggc
     4021 gctcacacgg atggacaccg tgtcggtggc gccgtcggtg gcctcgaccg gcaccaccgc
     4081 tgtcgcaccc gccggcaccg gtgcgcccgt catgatgcgg tgtgccgtac cgggtttcag
     4141 cgtgagtggg tcggtgcggc cggcggggat gtcctcggcg acgggcagca acaccggctg
     4201 ctcgggtgtt gcggcggcga cgtcggcgtc caccacggcg tatccgtcca tcgccgaatt
     4261 gtcgaacccc ggcagcgaca gcggcgccac cacgtcgtcg gcaagcgcca ggccgagcgt
     4321 ctcggccagc ggcagggtga ccggtggctg gccggtgatc aggccggcga cgacgcgccg
     4381 gtgttcgtcg acggtgcgca tcaaacgggg tacgtcacgc cggtgagctc ttcagacacc
     4441 gtccacagtc ggcgctggag gtcctcgttt tgcgactgtg cgcttgaccc aacgactttc
     4501 gggtggccgc gcgtttctcc gatgccgtcg ggcccgtagt attggccacc ctgcaccccg
     4561 gggtcggtgg ctgcgcgaag ggtcggcagc gcacccatgt cggggggctg cgtgaaaggg
     4621 cgccaggcga agtcgggaat gaaaccgggc aggtgtcgca tgagctcggt gtccgagaaa
     4681 ccgggatggg cagccgccgc gatcgtgggg gagcccttgg cgttcagccg tcgctgcagc
     4741 tcgtaggtga acagcaggtt cgcgagcttg gattgcccgt atgcctcaac gcggttgtac
     4801 ttgcgctcca attgcaggtc gtcgaaatgg atccgggcca ttaaccggtg gccgacgctg
     4861 ctgatcgtca cgacccgcga cccttcgacc ggcaggatgt tgtcgagaag ttgacccgtc
     4921 aacgcgaagt ggccgaggtg gttggtgccg aactgcatct cgaagccgtc tttggtggat
     4981 tcgcgcgtcg gcacgtacat cacgcccgcg ttgttgatca gcaggtcgat gcgcgggtgt
     5041 gtggcacgca gttcgtctgc cgccttacgg acgctgtcca gcgacgtgag gtcgagctgc
     5101 tgcagcgcga catcggcgtt cgggctcgtc gacttgatcc ggtcggcggc ctcgttgccc
     5161 ttgtcgaggt tgcgcaccgc cagcacgacg tgtgcgccct tgtcggcgag gacggccgcg
     5221 gtgtcatatc ccaacccgga atttgcaccg gtaacgatgg cgacccggcc ggtttgatcc
     5281 ggcacatcgg ccgcggtcca cttgctgctc atgggctcaa catactcagc cgcccatggc
     5341 ttgcgtcgcc ggttagtttc gttgtcgtgg gtaaacgaat tgtcgcaacg gtcctcggct
     5401 cgctgatcgg tgcggttgtg ctgttcctgg gtctgctgct ggccgggctg cggtggcagg
     5461 tgacgccggt ggtggacttc gtccggcgga tgaacaagtc ggtgtcgaac ccgcgggtga
     5521 tgcgtactgc gggcacgacg cagacgcaga catcggtcat tgagcatgtg gggcgcacgt
     5581 ccggccggac ctaccaaacg cccatcgacg tcatcccgac taggactggg ttgttgatcg
     5641 ccctgccgta cggtgcgcgt gctgactgga cgcgcaacgt gctggccgcg ggcgctgcga
     5701 cggtcgtcac gcagggcgag cgggtgaccg tagaccggcc gaccatcgtt gccgcggccg
     5761 acgtggcaag cctgattccg aaaaggacga tacgaatgct gcggttgttc ggtgtccgag
     5821 aatgtctgca tctcgagaaa tcatgaatct ggttgagccg gccaacccgc cgagccgcaa
     5881 ggcgccgctc gtgtgggcga tcggcgcggc cattccgtgg ctgatgctgg cggcgggtca
     5941 ggcggtgtgg ttcgcgatcg atccgcggcc gggctggttg cacgcgttgg ccgccgcggc
     6001 cacggtagga ggcattgtcc tgttcgtcgc ggtggttccg ttgtggcgat atcgcgtgca
     6061 ccgctgggat attgacccca aggcggtgta cacgcggacg ggctggctgg tgcaggaacg
     6121 ccggatcgcg ccgatctccc gggtgcaaac ggtcgacacc gaacgcggcc cactggacca
     6181 gctgttcggg ctggcgaatg tcacggtgac gacggcgtcg tcggcgggcg cggtgcgcat
     6241 cgtcgcgttg gattccgatg tcgccgaccg ggtcgtcgct cagctcaccg acatcgctgc
     6301 actcggcggt gaggacgcga cgtgagcacc cgaagacgag cgcgagtgag gatcgcagcg
     6361 aggaacgaac gaggaccgga gcgaggggga gtcgaggcat gagcaccgat gccgggcagg
     6421 tgtggcagcg gctcagcccg cgcatgctgc tggtgcatcc ggtgcacgag gtgctgcgcc
     6481 agataccgct gctgatcggg tctgtggtgc tcgggtcggc gaccggcaat cccctgtggg
     6541 ggctggtcgg gctggcgctg atcgtcggct acggcgcggc ccggtggttc accaccacgt
     6601 accggatcgg ccccgacgag gtacagctgc gcaccggtgt gcttcagcgc aaggttcttt
     6661 cggtgccgcg caacaggatt cgctcggtgt cgaccgatgc gagactgttg caccggctgc
     6721 tcgggttgac cgtgctgagg ctgagcacgg gtcaggaggc tcggggcgac gccgcattcg
     6781 ctctcgacgc ggtcgaggcc gagcaggtgc cacggctgag ggcgaccctg ctggcggatt
     6841 cgctggcgcc cgtcgagcag gaggcgtcgg gccgtgaact ggcacgctgg cagccgtcgt
     6901 ggctgcgcta cagcccgctg agtttcaccg gactggcgat gatcgcggcc gcggttgggc
     6961 tggtgtatca ggcgggtgcc ggtgcggcac tgcaggattc gcggctggcc cggtcgggtc
     7021 tggatgcggc cgagcgcttc ggggtcgccg cgagcgtcat cctggcgggg gtggtggtgc
     7081 tggtcgcgtc cgtggtgctg tcggtgctga ggtcgttggt gacctacgga aacttggtgc
     7141 tgcgtcgcga cgccgaggtg ttgcacctcg agcatggcct gctgcggcgg cgcgagcgca
     7201 cgttcgacat gcgcaggtta cgcggcggca cgctgcgaga gccgctgttg gtgcggttgt
     7261 tcgggggcgc gcggctggat gcggtgatga cgggtgtcga tggtgcgggg gaggcgtcgc
     7321 tgctgttgcc gccatgtccg cgagcgacgg ccgaggaggt gctgtcagag ctgattgcca
     7381 cgaccgacgc ggacgagagg agcaccaaat atttcgaccc agtcaccggc ccgctgcgcg
     7441 cgcatggcgc ggcggcgacg cgcagacgct ggacgcgggc attgacgctg cccccggtgc
     7501 tgctgatcgc gatggttgcg gtgccggcac cggggtggct gtggccggtg tgggcggtgc
     7561 tgaccgtgtg ctgcgcgctg ctggcagtcg accggtcgcg ggcactcggc catcgggtgg
     7621 tggacgacga atggctggtg gcgcgggcag gcagcctgga caggcggcgc gactgcatcg
     7681 ccgccgcggg gatcatcggg tggaccgtgc gccagacact gctgcagcgg cgggcgggcg
     7741 tggcgacgtt gatcgctgcc accgcagcgg gggtcaagcg ctatcaggtg atcgacgtgc
     7801 cggcggacct ggcgtggacg atcgccgcgg ccacctcgcc gtgggttgcc gacaaccagt
     7861 gggctaagcc ttgacgggtt cggcggtcag ctcgccgcgc tgaatctttg tcgagagcgc
     7921 ccgccgccac ggaaaccagc acctgcgcac cgtccacacc ttgccatcgg gatcggtaac
     7981 ccgggacgga cctgccatgc gattatcact gccgcgtcac aatcgagggc atgatcacgg
     8041 gcttggcaca cagcggcgtg tgcgttcccg actgtgaggc cgccgtggcg tattaccgcg
     8101 acgtgctggg attcacggtg ctctcgccgc cgtatgtgat ggacggcgac gccatccgcg
     8161 ccgatatggg cgaattgttg accgatccgg cgatgaaggc cgcgatcgtg gggttcggcg
     8221 gggacggcga ccgggtgctc gaggtcatcg agtacctgcg cgccgacgga agcgacgccc
     8281 gtcgagagag gctcgccgac cccgggctct cgcacgtcgg cctgatatgt gacgacgtgg
     8341 acgagactcg gaaggcgctg gaggacaagg gagttcactt tctggtcgac ggtatcgccg
     8401 acatcgccgg ggtccgcacc acgtggttcg ccgatccatg gggtgtgatc ttcatcctcg
     8461 tcgagaagag caagcctgag cgtccgtact tcgcacagtt cggatagcct ccgacttgcg
     8521 ctgattcgtt tgccgtttac tgtcgggcca tggctatcgg cggtgtgctc ttcgacatcg
     8581 acggcgtctt ggtgacgtcg tggaagccga tcgagggtgc cgccgagacg ttgcagatga
     8641 tggcgcgcaa tcagattgca tgctcgtatc tcaccaacac cacgacgcgg acccgggtgc
     8701 agatcgccga gctgctcact gaggcgggca tgcaggtgcg cgccgacgaa gtcatcaccg
     8761 cggctgtgct gaccgcggag tatgtccgca accgctatcc cgacgctagg tgcttcctgg
     8821 tcaacagcgg gcagatcgcc gaggacatgc ccggcatcga catcgtgtat tcgagcgagt
     8881 tcagcgggcc gaaggcgccg gacactcccg atgtggtgct gctgggcggg gcggggccgg
     8941 agtacagcca tctgacgctg tcgtgggtgt acgactggat ggcgcagggc gtgccggtgg
     9001 tcgccatgca tcgcagcacc gcatggacca ccaccgacgg gttgcgcgtc gacaccggta
     9061 tgtacctgat cgggatggaa gagacgtccg gccgcaaggc caccgccgtg ggaaagcccg
     9121 cgcccgaggg gttcctcgcc gccgcgagtc ggctcggcgt ggatcccgac gagatgtaca
     9181 tggtcggcga cgacctcaac aacgacgtgc tggccgcgca ggtggtcggg atgaccgggg
     9241 tgctggtgcg caccggaaag ttccgccagg acacgctgga ccgttgggcc gcagacgaat
     9301 tcgcgatgca gcccaatcac gtcatcgact cggtggcgga cctgcccgcg ttgttggcac
     9361 tctgatttcg cgccgacact gcggggagat cgcaactttc gccgaaatcg cgatccgtgc
     9421 gcagtttcga gggagtatcg cgattactta ttgcgccgat gcaggtctgt attcatatga
     9481 ctgaagaatc catgagcgtc acggcgacga tcaatgcgcc tgcccagaaa gtgttcgacg
     9541 tgttggttga cccatcgact cacgaggcaa tcgatggcac cggatgggtc cggcagtcgc
     9601 tcgacggcaa gcgaatcacc gagaccggtg aggtcttccg gatggcgatg taccacgaga
     9661 accatcccaa caagaactac gaaatggcga accgcgtcga agttttcgaa ccgccgcacg
     9721 ccgtcgcctg gcagcccggc caaggaccgg acgacgccaa tctcgacttc ggcggatgga
     9781 tttggcgcta cgacctcaaa cccgtcggct ccgcccaaac ggaggtcacg ctgacctatg
     9841 actggtcacg agtgccgccg tctacccgcg agcacatcgg attcccgccg tttgaagccc
     9901 agcacctgga gaactcgctg aaacacctgg cggagctgtc acaatcccgg tagcgacggc
     9961 aatgacggga aggtgatctc cggggtgttc accggcgcct cagtcgcagc cggcgcctgc
    10021 gtcgtcatcg gcggcggagt ggccaccggc gcctgggtcg tgcttggtac ctgagcggcc
    10081 gtctgctcct gtgtcgtcgt tggtggttcc gacgatgacg gcggttgtga ggatgtcggt
    10141 tcggtggatg tcggagacgt actcgtcgcg gatgtactcg tcgtcgtggt cggtgcgctc
    10201 gtcgtcgtcg gcggcgaggc ggaggtggac ggagcggggg cggccggcgg tggcgccaca
    10261 ggcgcctggc ccgccctgcg cggtgcgggc ggggatgcag gccgcggctg aacccggggc
    10321 gccgctggtg cctggcggac gatcacgggc gcgggtgctt gctcggcggg gggcggggta
    10381 ccggcggcgg ggggcggtgc ttggggttgc actaccaccg ggaccgctga cggtgcgggc
    10441 gccggagcgg ggctaccgct gctgctcgtc aaggagtagg tcaggccgcc gccggtgagc
    10501 aacacggccg cggcggccac gccaaggagc aactgtggac ggcggtacca cgcgaatggc
    10561 gcctcttcgt cgacggcgtc ggggtcgcgg atgaactcca gctgcggtcg cacaggatcg
    10621 gccgtttcgc ggtccgcatc gtcggtttca cctgagtacg gcaacggttc gtcggcggcg
    10681 acgtcgtccg accacgccag cgcccgaaat gtctcggtcg cggggctgtc ggcggcggcg
    10741 ccggccgcgg caacaccggc ggcgacgcgt gcggctggcg ccatcgcggt ggccggagct
    10801 gccgggcctc gcgccgcgat gagcgccgcg ccagtcgccg gggcaagctc cggtcgcggc
    10861 gtcgtcacca cggggatgcg caggtgttgt gagagtcgtt gggtcaccag cggaatattg
    10921 gctccaccgc cgacggttgc gatggccgct acgttggcgg gcggaattct gttgcggtcc
    10981 aacgtatctt ccaatgcggc gatcacacca cccaatggct tggccaccag gtcttcgagc
    11041 tctgcccggg tcagcctgac attggaccgg aacgccggca gctcggctgt caccgtggcg
    11101 acggtatccg ccgacagcgt ctcctttgct tttcggcatt ggtcgcgcaa tcggctcaac
    11161 gaaaggaccg cgccagtgct atcggggtcg atgttcgccg cctcggcaat gccggacagg
    11221 atccgcgcga ggatgctctg atcgatctgc tggccgccaa aatcggtaca gcgcatggtc
    11281 tcaccgatcg gtgcccagcc cctggcggcg tcgcacaacg tgatgctggt cccggttccc
    11341 ccgaaatcga gtaacgcgac gacaccgcga gacggcagcc cacggtggtg ctgcaacgcc
    11401 gaaagcgagg cggtcgcgtc ggagatgagc ggcggtggac ccgcggggaa cagccgttgc
    11461 ttcgcggaca gatcctgatg gaagctcgcc ttcgcacgcg agtcccaatg cgcgggaacg
    11521 gcaatcgcga tgtcgtgtgg tggccggccg ctttcgggaa tgcgcattag gctctcgagc
    11581 gcctcgacca gcaaccgccc cggacggtga gttgagccat cggctgccac cagtggaatc
    11641 ggatccccta ctcgctccac gaagccggcc aacaccatgc cgtgttcgcc aggtggcgag
    11701 ttctgctccg gtgcaccgac ttcgggcgcg cggtcgggat acagggtgag aatcgagcgc
    11761 cctagcgccg gcggccagcc ggagcgtgcc gcgccgagat tggtcatgcc caccgacaac
    11821 cccaacgagt cgttcatgtc caccccctcc gcgcggctgc catgtcgcga caaagtttgc
    11881 accgccaacg agcgcgtgag gaggggcgac cgcgaaatgt agggaaacac ctgattcaac
    11941 ggtcgccggg cggcaatagc ttgccaatta accggctgag ccggcatgca tgtttccacg
    12001 cggggaagga cgccaccatg gccaatctca tcgactacgt catggatctg ttccgtgacg
    12061 agggccaagc acaatcattc gtcgacgatc cacagggtgc tctggccgcc atcggcctgc
    12121 ccaacgtgac tcccgagcag atccaggccg tcgccgcgat ggccgcgccg ggcggggcga
    12181 tggccgcacc ggctgggacg gggatgaccg ctggcgaccc gatgggcact cttcgccagg
    12241 cggtggccag ccaccacggc atgccggcgc cggccggcgg gggtggtggc gcgccgcatc
    12301 ccgccgaaca cgttcagcag gccgccgcgt tcgcgccgac gtcgcatgcc gccgggctcg
    12361 ccgggtcaac acagggcgat gtcacgcacg cgacgttcgc gccgcagtcc aacgacctgt
    12421 cgaaccgcga gaccaacgtc gacagccaca acaccacagg cgatttcatg agcccgcaca
    12481 ccgatggtgt gcagcagggt ggcggcggat tcaacctcgg gatggacttc ggcgacatca
    12541 ctctaggtga caagaacacc gcgtcgggag atggcgcggt cgcgatgagt ggcgacaact
    12601 cgggcagcat cgtcagcggc gacggtgcgg tgctcggtga cggcaatacc gttctcaacg
    12661 gcaacatcga tgccggtgac ggctcaccca tcacgtttgg cgacggcaat gaggtggacg
    12721 ccacctcgat gaaggccggc ggcgacatga tctccggcaa cgaagggccc gtgctcaacg
    12781 gcgtcgacac cggtggcggc agcctcggcc tcgacaacag ctcaacggtg ggcggagatc
    12841 agaacaacac cgacatcagc ggtgagatcg gctccatcga tggggatctc gacaccagcc
    12901 accacgacga caagtcggtc gacgcctcgg accactcggt caccacggtc gacagccacg
    12961 atgtgtcgga cagctacaac gatcagagca agcaggaaac cactgccggc ttcgacggcg
    13021 gcgacgtcgg cggcgacttc agcctcggcg gaatactgcc gttctgatcg gcggctgcga
    13081 cacaccggcg acgggggacg tcagatgacg cagccggcca acgacccgcg caacgtcaag
    13141 ctgctcgttg agctcatcga ccacaccacg aagattgctg agctcaacgg tcgcggtgac
    13201 ctgtcggagc ggttggcgcg cgctcggaag cggatcagcg atccgcggat ccgcgtgatc
    13261 gtcgccggtc agctcaagca gggtaagagt cagctggtca actccctgtt gaatctcaat
    13321 gtctgcaagg tgggtgacga cgagtccact gcggtcgcca cggtggtcag ctactccgaa
    13381 aagccctggg cgcgtttggt tgtggagcaa cccgacggct cagaacccga aatcatcgag
    13441 ataggagtcg gcgagctcaa gaacgatctg cgccgagcgc cgcaagcggg cgggcgagag
    13501 gtgatccgtg tcgaggtcgg cgcgccgagc ccgatgctca agagtgggtt ggccttggtc
    13561 gacacgccag gtgttggtgg ttacggtcaa cctcacctag cggccacgct ggggctgttg
    13621 cccgacgccg acgccgtcct gatggtcagc gacctaagcc aggagttcac cgagcccgag
    13681 atgacgttca tgcggcaggc attcgaaatg tgcccggtcg caacgatcgt cgggacgaag
    13741 accgacatct acccgcactg gcgcgacatc gtctacgcca acaccacaca cctgcaacgc
    13801 gcgcgcatag ccacaccgat aatcccggtg tcgtcgatcc tgcgttcgca cgcctgcgcc
    13861 acaaacgaca acgagttgaa cgaggagtcg aacttcccgg ccgtcgtgag gttcctggcc
    13921 gatcacgtgc tctcccgcga gaacgaccgg attcgcgatc aggtcctcgc ggagatcgcc
    13981 tcggctgccg agcatctggc cttttctctg aacaccgaat tcgagtccat caacgatccg
    14041 gatgcgcgcc agaagctcac cgaagatctc gagcggcgga aacgggaagc gcaggatgcc
    14101 ctcgcccaaa cggcgttgtg gcagcaggtg ctcaatgacg gcatcgccga tctcaccggc
    14161 gatgtggatc acgacctgcg cagccggttc cgctcgatca cccagcacac cgagaaggtg
    14221 ctcgatggct gcgatccgac cgtgtgctgg gtgcagctcg ccacggaatt ggaggacacg
    14281 gtagcgacgg cggtcggtga caacttcgtc tgggcgtatc agcgcgcggc ggcgctggcc
    14341 gatgaggtcg ggcagacgtt cgccgaggcc ggcctcgaag ccatcgatgc gccggtgatc
    14401 aacgccagcg agatcggcgc gagctttggc ggagtcaacg gggtgggcag acttgatgcc
    14461 aagccagttg gcaagggcca gaaggtgtta acaggcgtcc gaggctcata tggcggcatg
    14521 atgatgttcg gcatgctgac ctccctggcc gggttgccga tgatgaaccc gctggccgtc
    14581 ggcgccggtc tattgctcgg ccgcaaggcg tacaaggaag accgggacaa tcggctgaac
    14641 cgggcccgcg ccgaggccaa gatgaacatg cgccgattca tcgatgacgt atccttcgtg
    14701 gtcaccaagg agtcacgtga ccggctcaag ctggtccagc gccaactgcg cgatcactac
    14761 cgcgacatcg ccaaccaggc caaccgatcg ctgaacgaat cactccaggc gaccatcgcg
    14821 tcggccagga tgaaggaagc cgaaagcaac acgcggataa aggaactcga acgccagctc
    14881 aagatcgttc gagcagtgac cgaccacacc acaaagctct ctggtggatc ggcgccgaaa
    14941 actccgacat caccacaagc agcacccgcg gtggcggctg tcggatgact gttaggagat
    15001 gccatgtcca taggtgattt catcgacgac ccattcgggg cggtggccaa ggcgggactc
    15061 gatgccttcg cgcagggtgg gatcagcggc gaaaacgccg atgttgtcgg gggattcgag
    15121 gtgcccgatg tcagatcgat gacgccgttc ttcggcgatg acaagtcgga cccgtaccag
    15181 ctgggcggcg tcggtgagca gccgtcggag ccgtacgtgg acaactccgg gccgcagatc
    15241 atccatccgg atccgatgcc cgacaccgcg cccgaacctt ccaccgcagc cgacgacgcg
    15301 gctggttacg gcgccgccgg agaagttgac agcggaacgt acgacatctt ttgaacccga
    15361 catccataca cagaaggaga aatgcacatg tctgtaagtg atttcatcaa tgacccgttc
    15421 ggcgcggtag ccaaggcagg cgtcgatggc atgcctgacg aggtgaagga aaccgtgcac
    15481 gacgtcgcgc agcagatcac caatcccgcc atgccggatt cgggtgctct taccgaggac
    15541 ttcgggaacg cggatgggtc tttcggcgac gctgggaccc tcggcaaggt gagcgcaccg
    15601 gaaatgcaca ccctcggcga gaacacgttt ggcgggatcg atatcgcgga ccaaagcatc
    15661 attcacccgg atccgtatgc ggatcccgag ccgccgcact acgacgtcga ggtcggcccg
    15721 gcgaccatca tcgacccgga ggagggcacc gcgggcgaac aagcgcagta ttccggcgcc
    15781 gatgggtaca tctacacctc ggactatggc ggttcggccg cggaagaagc ggcgggctcg
    15841 ggtgtcagcg cctacggccc ggacacggac cccaactatt cgatctcccc ggttggggag
    15901 gacggacccg cggcagccga ttacggcccg ggcgccacgg gtgagctcgc cggcgcgggc
    15961 ggtgaggtga ccaccgaaga cgacagcttc ttctgattgc ccgtcagcta cgcctgcagt
    16021 gtgcgcaccg cttcgatgcg ggccttgagc tgatccgtcg tcgccgccgc aatcggcggg
    16081 ccgccgcaca accggcgcag ttcgttgtgg atccagccgt gcggctttcc ggtcctgtgg
    16141 tgggcgattg acaccagcgt gttgagttcg cggcgcaagt cccgcagttg gccgtgcatc
    16201 gacggccgcg gcacctcacc gcttgcagtt cgcttcgaaa gctgttcgtc ttgcctgcgc
    16261 cgcagtagat ctcgcatctg gtccgcgtcg agcagaccgg ggataccgag gtaatcggcc
    16321 tcctcctcgc tgcccgccgg ggtcgccgtg ccgaacgaag agccgtcgaa gatcacctgg
    16381 tccagctcgg cgtcggcgcc caacgattcg aagccgttgt ccaattcgct tggctcggac
    16441 ttgcgtcgct cgacgtactc gtcgtcgcgt tgcgactccc ggtgcggctt gccgagcacg
    16501 tggttgcgct gggcctccat ctcactggcc agcagcagca ggttgggcac cgacggcagg
    16561 aagatgctgg ccgtttcacc gggttggcgg gagcgcacga agcggccgat cgcctgcgcg
    16621 aaaaacaacg gcgtcgacgc gctggtcgcg tacaccccga cggcaagccg tggcacgtcg
    16681 accccctcgg acaccatgcg caccgcgacc agccacgggc tcctcgcggc cgcgtaatcc
    16741 gcaatgcggg ccgaggatcc ggggtcgtcg gacaacacca ccgtcggcgc ttgaccggtc
    16801 agcttggtca acacctccgc atacgcgcgg gcggccgtct ggtccgaggc gatcaccatc
    16861 gcacccgcgt ccggcatgcc gccctcgcgc aactggttga gccgtttgtc tgcggcctgc
    16921 aacacggcgg gaatccactc gccggccggg ttcaacgcgg tccgccacgc gcgggcggtc
    16981 tgctcggcgg tcaggggctc gccgaggcgc gccgcgtgtt cctcgcccgc gctgtcgcgc
    17041 catcgcgcct cgcccgagta ggccaggaag acgaccggcc ggacaacgcc gtcgcccagt
    17101 gcctcggcat agccgtaggt gtggtcggcc cgcgagcgca tcaggccgtc gccgtccggc
    17161 tcgtaggtga cgaacgggat cgcgctgtcg tcgctgcgga acggcgtgcc ggtcaacgcc
    17221 agcctgcggg tcgcatcggc gaaggcctcg cggatcgcct caccccagct tttcgcgtcg
    17281 ccgccgtggt ggatctcgtc gaaaacgacc agcgtgcggt agttctcgat acgcacacga
    17341 tgtcgggtcg ggtgactggc cacctgcgcg tacgtgacca cgacaccgtg gtattccgac
    17401 gacgtctgcg ccgacgaatt ggtgaacttc ggatcaagcg cgatgccgac cctcgccgcg
    17461 gccagcgccc actgcgtctt gagatgttcg gtcggcacga cgacggtgat ccgctcgact
    17521 gtgccgtcgg ccagtagctc tcccgcgatg cgcagagcaa acgccgtttt gcctgcgcca
    17581 ggggtggcca cggcaaggaa atcgcgcggt ttggcgccaa gatagcgcac caatgcccga
    17641 cgctgccagc ctcgcaaagc ctgggtgtcg ggcgcagcat atgcccgcac ccaagactcc
    17701 tcactgatcg gacttcagcc tagcgcaagg gattacgggt gcgcacttct cggcgtgtcg
    17761 gctattcgac gaagaagttg tcgttggcga tgaaccactc gcggcgctcg tcgtcagaca
    17821 tgtcggcgag ctgggccatt ccctcgaagt agtgctcacg cggcgcgccg ggagcaaaca
    17881 gcatcaagat tgacgtcggc tcctcggcct cgttgcggaa gccgtggatg ccgccgggtg
    17941 ggacgtagag aaagtcgttc ggatgcccgt cgatccaatc ggtgccgtcg tacagcttca
    18001 ccgttcccga cagcacgaag aaggcctcgg acatggcacg gtgaaagtgc gggcccggcc
    18061 cgccgccctt cggggcgatc tccacccggt acaggccgta gtcgccatcg gtggcttgtt
    18121 ggttcgccag gtagtggtac ttcaccagac cgaacgagtc gtagtccggt ggttcagtgc
    18181 ctcgccgaag ccaggcactg acctccggtt cgtctttggt gtatcgggct ggcgggtacg
    18241 gcggcacgat tagcgacatc gcttgccagc ctgccacatt cagccgacgg gagacaccac
    18301 cgaagtgagc tcctccgaca ccgcccacag ccggcgctga gcgtccacgt cgtgtgacgc
    18361 cacgctcgac gcgacgacct tcgggtagcc ccgttgctcg ccgaagccgt ccggcccgta
    18421 gtactgcccg ccgagcacgc ccgggtcggt ggccgcgcgc agcgtaggca gcgcgcccat
    18481 gtcggggcct tggaaaagcg gtgccagtaa ggtgcccagg ccgcgtatcg cgacgggcag
    18541 attgcgcatg agttcggtgt tggagccgcc ggggtgcgcg gcgacggcga tggtgtgcgt
    18601 gccgtgcagg cgccgctgca gttcgtaggt gaagagcagg ttcgcgagct tggcctggcc
    18661 gtaggcacgg acccggccgt agtcgcggtc ccactgcagg tcgtcgaagc ggataccgcg
    18721 ggcgaaccgg tggccttggc tgctgaccgt gacgatgcgg gagcccggca ctgccaacac
    18781 gcgatcgagc agcaggttgg tcagcgcgaa gtggcccaaa tgattggtgc cgaactgcaa
    18841 ttcgaagccg tccttggtgt gggacttcgg cgtcatcatc acgccggcgt tgttgatcag
    18901 caggtcgatc tggtcgtgct ccgagcgaag ttcatcggcg gcgttgcgga cggactccag
    18961 cgacgtcagg tcgagctcct gtagcgccac gctggcgccg ggggtgcgcg ccgttatgag
    19021 gtcggccgcg gctttgccct tttcgagatt gcggacggcg agtacgacat gtgcgccctt
    19081 tgcggcgagc gccgtcgcgg tctcgtagcc gaggccggtg ttggcgccgg tgatgacggc
    19141 cgtgcgaccc gtctggtcgg gaatgtcggc ggtggtccag ttgctcatgg gaatctccta
    19201 caatgggtta aacggggcgg gcgctccgtt tggtgacgac tatacggaac gcgcgccccg
    19261 cttatctatt cccgaggtga tgtgagatgg ctgaactggc ccggccgcta cgcgccgacg
    19321 cggcgcgtaa tcgtgcgcgg gtgctggagg tggcctacga gaccttcgcc gccgagggac
    19381 tgggcgtgcc gatcgacgag atcgcccgcc gcgccgccgt cggcgccggg accgtctacc
    19441 ggcatttccc caccaaggag gatctgtacc gggcggtcat cgaggaccgc atcaaccaca
    19501 tcgtcgatga cggccgcgcc ctgctggccg acgcggaccc gggggaggcg ctgttcacct
    19561 tcctgcggtc gatggtgctg cagtggggcg cgaccgaccg cggcctggcc gaggctcttg
    19621 ctgggctggg gatcgacgtc aaagacgcgg cgcccgaggc cgaggaagcg ttcctgggac
    19681 tgctcggcga gctgttgcga gcctcgcaga aggcgggcac ggtgcggcgt gacgtcgagg
    19741 tgccggacgt gaaggccctc atggtcggag cacaggcgat ccagacctat aacgcggacg
    19801 cggccgagcg gctgacggag gtcgtcctgg acgggttacg cgcggggtga accgtcgccg
    19861 ggcgaccttg cactcgccct ggtcgagtgc taagaatgca gttggcactc gcgaccggtg
    19921 agtgctaggt cgggccggtg aggcaggggc cgcacagccc acagccgtcc gtcgcgggca
    19981 ctgcacccga ccaacaagac gtgcatcccc tatccggagg aaacacttcg caatggccaa
    20041 gacaattgcg tatgacgaag aggcccgtcg cggcctcgag cggggcctga acagcctcgc
    20101 cgacgcggta aaggtgacgt tgggccccaa gggtcgcaac gtcgtcctcg agaagaagtg
    20161 gggcgctccc acgatcacca acgatggggt ttccatcgcc aaggagatcg agctggagga
    20221 cccgtacgag aagatcggcg ctgagctggt caaggaagtc gccaagaaga ccgacgatgt
    20281 cgcgggcgac ggcaccacca ccgccaccgt gctggcccag gcgctcgtgc gcgaggg
//
LOCUS       DASRJA010000010         6953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2246102,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000010 DASRJA010000000
VERSION     DASRJA010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2246102"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..877
                     /gene="otnI"
                     /locus_tag="VFC01_00500"
     CDS             104..877
                     /gene="otnI"
                     /locus_tag="VFC01_00500"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HZN78158.1"
                     /translation="MPRFSANLSMMYTEHPFLDRFAAAAADGFTAVEYLFPYEFPAPQ
                     LRGLLDCNGLQQVLFNAPPGDFDAGERGIASIPGREAEFRNGFHRALDYAEAMDCPRV
                     HVMAGLVPAGAERDEQVKVYRDNLGWAAGRAQTRGVDVLIEPINQRDMPGYLLSLQDE
                     AHQIVVEIGAPNLKVQLDLYHCQITEGDLTMRLRADVPTGRVGHLQIAGVPDRHEPDR
                     GELAIDYLLAVIDEVGFDGWVGCEYRPAAGTSDGLGWMK"
     gene            874..1860
                     /gene="denD"
                     /locus_tag="VFC01_00505"
     CDS             874..1860
                     /gene="denD"
                     /locus_tag="VFC01_00505"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: protein motif:HMM:NF043036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HZN78159.1"
                     /translation="MRVVITGGFGFIGTMVARELLTTGTFQGRELTELVVADRFVPRD
                     DDAITADPRTTVVEGDLLETMSTIFARRVDAVLHLASAVSAEAEADFDLGMTANVQST
                     LALLEAARHQSGEPATVVFSSSVAVYGSDPALPLPDVVSESTLPTPQSSYGAQKFICE
                     QLVADYTRKGFIDGRVARLMTVAVRPGKPNAAASGFLSGIIREPLAGIATVCPVRPEL
                     SVALASPRRTVEGLLRVAQVEGGDDRGKLSGRIAVNLPALTVTVAEMLDTLRRVAGNA
                     VADLVSVEPDPAVEAIVGSWPSRFDNSRAAALGLTGDASFADVVAQYRDDNL"
     gene            1876..2610
                     /locus_tag="VFC01_00510"
     CDS             1876..2610
                     /locus_tag="VFC01_00510"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HZN78160.1"
                     /translation="MSGGVSLVDRVAEVISDEITSGRLSPGDRLPSEVQLVEQLGVSR
                     TVVREAVSRLRNAGLVEPRQGAGVFVLAPRMKPLDLSSEAQQTKASVLQVVEVRRAME
                     GEAAALAAIRATPEAIRRIRAALEAIDDDVRAGGDGVDADLAFHQSVVDSTGNPVLAE
                     TLRYIGSLMRSGMRVTRANEARRADFTAQVRREHQLMLEAIEGGDPEAARAAARQHME
                     HAAERLQQADDRFWSESGVLAFEANS"
     gene            complement(2883..3344)
                     /locus_tag="VFC01_00515"
     CDS             complement(2883..3344)
                     /locus_tag="VFC01_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN78161.1"
                     /translation="MAATASFSLYKATPAGAEETMEISPDESRPADEGSADHFSGNVS
                     VKPLFNPNAVRTFSSAEVTFTPCARTAWHTHPAGQTLIVTTGTGWIQQWGGQKQSIKP
                     GDVIWTPPGVKHWHGATDSSAMTHIAIQDSVDGKPVDWLEHVTDEQYLQNS"
     gene            complement(3469..4005)
                     /locus_tag="VFC01_00520"
     CDS             complement(3469..4005)
                     /locus_tag="VFC01_00520"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZN78162.1"
                     /translation="MNQIRRVASREVYRNNWLTLREDDIRRPDGSPGIYSVIDKPTYA
                     LVIPRDGDRFHLVEQFRYPLGVRRWEFPQGTAPGQQDLDPIELANRELREETGLRAES
                     MELLGTLDVAPGMSSQRGRVFLATGITEGEHDREHEEQDMHSDWFTRAQLERMIRDGD
                     ITDAQTIAAWALFMLTGR"
     gene            4048..5451
                     /locus_tag="VFC01_00525"
     CDS             4048..5451
                     /locus_tag="VFC01_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HZN78163.1"
                     /translation="MLLDSLDPAAVTGGADIGDAVRIDGAVLSRSDLVGAATSVAERV
                     ADADRVAVLATPTATTVLAIAGSLIAGVPVVPVPADVGAAERRHIVTDSGAQAWLGEK
                     PAETDDLPHIPVRLHARSWHRYPEPHPDTTALIIYTSGTTGPPKGVVVSRRAVAADID
                     MLAEAWRWTSDDTLVHGLPLYHVHGLVLGLLGSLRIGNRFVHTGKPSPAAYAAAGGSM
                     YFGVPTVWSRIVDDGDAARALKSARLLVSGSAPLPVPVFDGLARLTGHQPVERYGATE
                     SLITLSTRVDGERRPGWVGLPLTGVQTRLVDDDGATVPHDGESIGRLQIQSPTMFDGY
                     FNRPDATAEAFDGDWYRTGDVAVIDDGGMHRIVGRESVDLIKTGGFRVGAGEIETALL
                     GHPGVVEVAVIGAPDDDLGQRIVAFVVGDAEPQALIDYVAQELSAHKRPREVRLVESL
                     PRNAMGKVLKKELTGWL"
     gene            5442..5792
                     /locus_tag="VFC01_00530"
     CDS             5442..5792
                     /locus_tag="VFC01_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN78164.1"
                     /translation="MALTFDEICVDAHDPGALGAWWSKVLGWRHYVDKDGDVVLDAPT
                     GAGPNWLFIAVPDDKVVKNRIHLDFRPDDQQAEVDRVIGLGARHVDIGQGEQTWVVLA
                     DPEGNEFCILAAHD"
     gene            5792..6366
                     /locus_tag="VFC01_00535"
                     /pseudo
     CDS             5792..6366
                     /locus_tag="VFC01_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
     gene            complement(6371..>6953)
                     /locus_tag="VFC01_00540"
     CDS             complement(6371..>6953)
                     /locus_tag="VFC01_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN78165.1"
                     /translation="PLSGSSVGAGRGALVAISASNLLAFLGQFTVYTFVTVLLLSSGV
                     SPTFVGPILLACGAGGLLAVWWAGRNLDRGPRRTALVLLAIVIGAVVALGGTWPTLVA
                     VIVATVVWNGAFGGLPSIYQTCAVRTHAASPELAGAWVNATANAGIAGGAAIGAGLLH
                     AAGLSSLPWVGASLIGLGLAVVVLSRKAFPARA"
BASE COUNT         1161 a         2274 c         2335 g         1183 t
ORIGIN      
        1 gatgttcatc ctctcgggga ttctgctgct cggcgcgctg tgtgtgttca gcacccgcaa
       61 ggacgtcgtc aaccgctgac gacttgtcac gaaggagctt gccgtgccgc gcttttcggc
      121 caacttgtca atgatgtaca ccgagcaccc gttcctcgat cggttcgctg cagcagcggc
      181 cgacgggttc acggcggtgg agtacctctt cccctacgag ttcccggcac cgcagttgcg
      241 cggactgctc gactgtaacg gcttgcagca ggtgctgttc aacgcgccgc caggcgattt
      301 cgacgcgggg gagcgaggta tcgcttcgat ccccggccga gaggccgagt tccgcaacgg
      361 atttcaccgg gcgctggact atgccgaggc gatggactgc ccgcgtgtgc acgtgatggc
      421 cggactcgtc ccggccggcg ccgaacggga cgagcaggtg aaggtgtatc gggacaacct
      481 cggctgggcc gccgggcgtg cccagacccg cggcgtagac gtgctgatcg agccgatcaa
      541 tcagcgcgac atgccggggt atctgttgag cctgcaggac gaggcgcacc agatcgtcgt
      601 cgagatcggg gcgccgaacc tcaaggtgca gctcgacctt taccactgcc agatcaccga
      661 gggcgacctg accatgcggc tgcgggcgga tgtccccacg ggtcgggtgg ggcacctgca
      721 gatcgccggg gtacccgacc ggcatgagcc agatcgcggc gagctggcga tcgactacct
      781 gctggccgtc atcgatgaag tcggattcga cgggtgggtc ggatgcgaat accgcccggc
      841 cgcaggcaca agcgacggat tgggatggat gaaatgagag tcgttatcac cgggggcttc
      901 ggcttcatcg gcacgatggt cgctcgcgaa ctgctgacca ccggcacgtt ccagggtcga
      961 gagctgacag aactcgtggt ggcggatcgg ttcgtcccac gcgacgacga cgcgattacc
     1021 gccgacccgc ggaccaccgt ggtcgagggt gacctattgg aaaccatgtc gacgatcttc
     1081 gcaaggcgcg tggacgcggt gctgcacttg gcgtcggcgg tgtcggcgga ggccgaggcc
     1141 gatttcgatc tcggcatgac cgccaacgtg cagagcacac tcgcgctgct ggaagccgcc
     1201 cggcatcagt cgggcgaacc ggcgaccgtg gtcttctcga gcagcgtcgc ggtgtacggg
     1261 tccgacccgg cgctgcccct gccggacgtc gtcagcgaat ccacgctgcc gacaccgcaa
     1321 tccagctacg gcgcgcagaa gttcatctgc gagcagctcg tcgcggacta cacgcgcaag
     1381 ggtttcatcg acggcagggt cgctcgactg atgacggtcg cagtgcggcc tggtaagccc
     1441 aacgccgctg catcagggtt cctgtccggg atcatccgcg agccgctggc cggaatcgcg
     1501 acggtctgcc cggtccggcc cgaactctcg gtcgcactcg cgtcgccgcg acggaccgta
     1561 gagggccttc tgcgtgtcgc gcaggttgag ggcggagacg atcgcggcaa gctcagcgga
     1621 cgcatcgccg tcaacctgcc ggcactcacg gtgacggttg cagagatgct cgacacgctg
     1681 cgacgggtcg ccgggaacgc cgtcgccgac ctggtctcgg tcgagcccga cccggcggtc
     1741 gaggcgatcg tgggttcctg gccgtcgcgc ttcgacaaca gccgggccgc cgcactcggg
     1801 ttaacaggcg acgcgtcgtt tgccgacgtc gtcgcgcagt accgggacga taacctgtaa
     1861 tttgtgctgc ccaagatgtc tggaggcgta agcctcgtcg accgtgtggc cgaggtgatc
     1921 tccgatgaga tcaccagcgg tcggttgtcg cccggcgacc ggctgccctc cgaagtgcag
     1981 ctcgtcgagc agttgggtgt cagccggacc gtggtccgcg aagcggtgtc gcggcttcgc
     2041 aatgccggcc ttgtcgagcc gcgacagggt gctggagttt tcgtcctcgc gccccgcatg
     2101 aagccgctgg atctgtcgtc cgaggcgcaa caaacgaagg catccgttct gcaggttgtc
     2161 gaggtgcgcc gcgcgatgga gggtgaggcg gcggcgttgg ccgcaatccg cgccacgccg
     2221 gaggctatac gtcgaatccg cgccgcgttg gaggcgatcg acgacgacgt acgcgccggt
     2281 ggcgacgggg tggacgccga tcttgcgttc caccaaagtg tcgtcgactc aaccggcaac
     2341 ccggtgctcg ccgagaccct ccgctatatc ggttcgctga tgcgcagcgg tatgcgcgtc
     2401 acgcgggcga acgaggcccg ccgggcggat ttcaccgcgc aagtacggcg ggagcaccaa
     2461 ctcatgctcg aagccatcga aggaggcgac ccggaagccg ctcgcgctgc cgctcgccag
     2521 cacatggaac acgcggccga gcgattgcag caggccgacg accggttctg gagcgaatca
     2581 ggagtcctcg cgttcgaggc gaacagctga cttgtgcgaa caaacttgag tatgttcacg
     2641 gctacgcgaa aagcattacg gcccaggcgc catccggctg cttgacgatc atccccgccg
     2701 ccgcttcggc gtgccttccc cggtcgacgc cgctctctaa gcgacacttg cggcagggag
     2761 gaatgcagga tgaacgcgct gctgctggtg tgcctgtccg tcggtgtgcc agtattcgca
     2821 ctttctctgc acgaccttca ggcgcggctc gagcggtggg attactcccg gcacattgag
     2881 gactaactgt tctgtaggta ttgctcgtca gtgacgtgtt cgagccagtc gacgggcttg
     2941 ccgtcgacgc tgtcctggat tgcgatgtgg gtcatggcgc tgctgtcggt ggcgccgtgc
     3001 cagtgtttga cgccgggcgg agtccagatc acgtcccccg gtttgatcga ctgcttctgg
     3061 ccaccccact gctgaatcca gccggtcccc gtcgtcacga tcagggtttg tcccgcggga
     3121 tgcgtgtgcc aggcggtgcg tgcgcacgga gtgaacgtga cttcggccga gctgaaggtg
     3181 cgcacagcgt tcggattgaa caacggcttg acactgacgt tgccgctgaa gtggtcggcc
     3241 gatccctcat cggcgggtcg ggattcgtcc ggtgagatct ccatggtttc ttcggcaccg
     3301 gcgggtgtgg ccttgtacag gctgaaggac gcagtagcag ccacgatcgt cactagcgtt
     3361 gttattggtt tcatgtccgt ccgcccttcg tcgatcaacg cagtccagcc aaccagcgtc
     3421 gtcggcagtg cgggagggct tatcgagacg tgttttgccg gtacccctct agcgcccggt
     3481 cagcatgaac agcgcccacg cggcgatggt ctgcgcatca gtgatgtcgc cgtcgcggat
     3541 catccgctcc agctgcgcgc gggtgaacca gtcgctgtgc atgtcctgtt cctcgtgctc
     3601 gcggtcgtgc tcaccctcgg tgatgccggt cgcgaggaac acccgaccgc gctgactgct
     3661 catccccggc gcgacatcga gagtccccaa tagttccatc gactccgccc gcaacccggt
     3721 ctcctcgcgt aactctcggt tggccagctc gatcgggtcg agatcttgct ggcccggcgc
     3781 cgtgccctgc ggaaactccc agcgccgcac acccaacgga tagcggaact gctcgaccag
     3841 atggaagcgg tcgccatcgc gcgggatcac cagtgcatac gtgggcttgt cgatcaccga
     3901 atagatgccg gggctgccgt cgggacgccg gatgtcgtcc tcgcgcaggg tcagccagtt
     3961 gttccggtac acctctcgcg acgccacccg acggatctga ttcacgcgcc ccagtatcgc
     4021 tgagcgccat atgggtaacc tcacgtcgtg ctgctcgact cactggatcc cgccgccgtc
     4081 acaggcggag ccgatatcgg cgacgcggtg cgcatcgacg gcgccgtgct gagccgcagc
     4141 gatctcgtcg gcgcagcaac ttcggtggcc gagcgggtcg ccgacgctga ccgggtcgcg
     4201 gtgctggcaa ctccgaccgc gacgacggtc ttggcaatcg ccggatctct gatcgccggc
     4261 gtgccggtcg tccccgtgcc cgctgacgtc ggcgcggccg aacgccgtca catcgtcacc
     4321 gactccggcg cacaggcgtg gctcggggag aagccggccg agaccgacga tctgccccac
     4381 atcccggtgc gcctgcacgc ccggtcgtgg catcgctacc ccgagccgca ccccgacacc
     4441 accgcgctga tcatctacac ctcaggcacc accggtccac ccaagggtgt ggtggtgagc
     4501 aggcgtgccg tcgccgctga cattgacatg cttgccgagg cctggcggtg gacgtctgac
     4561 gacaccctgg tgcacgggct tccgctctac cacgtgcacg ggctggtgct cgggctgctc
     4621 ggctcgctgc ggatcggaaa tcgttttgtg cacaccggaa aaccgtcgcc ggcggcgtac
     4681 gcggcggccg gtggctcgat gtatttcggg gtgccgacgg tgtggtcacg aattgtcgac
     4741 gacggcgatg ccgcgcgggc actcaagtcc gcccggctgc ttgtgtcggg cagcgcaccg
     4801 cttcccgtgc cggtgttcga cgggctcgcc cgactgaccg gacaccagcc ggtcgagcgc
     4861 tacggggcca ccgaatcgct gatcacgcta tccacccgcg tcgacggcga gcgcagacct
     4921 ggctgggtgg gcctgccgct gaccggtgtg cagacgcgcc tcgtcgacga cgacggcgcc
     4981 accgtgccgc acgacgggga atccattggg cgcctgcaga ttcagagccc gacgatgttc
     5041 gacggctact tcaacaggcc ggatgcgacc gccgaggcgt tcgacggaga ttggtatcgc
     5101 accggcgatg tcgcggtgat tgacgacggc ggcatgcacc gcatcgtcgg ccgcgagtcg
     5161 gtcgacctga tcaagaccgg cggattccgg gtcggggcag gtgagatcga gaccgcgttg
     5221 ctcggccacc ccggcgtcgt cgaggtcgcg gtgatcggcg cgccggacga tgatctcggt
     5281 cagcgaatcg tcgcgttcgt cgtcggtgac gcagagccac aggcgttgat cgactatgtt
     5341 gcgcaagagc tttcggcgca caagcggcca cgcgaggtgc gactggtgga gagcctgcca
     5401 cgcaacgcga tgggcaaggt gctgaagaag gagttgacag gatggctcta accttcgacg
     5461 agatctgcgt cgacgctcac gacccgggag cgttgggtgc gtggtggtcg aaggtgctgg
     5521 gttggcggca ttatgtcgac aaggacggcg acgtcgtact cgacgctccg acaggtgccg
     5581 gacccaactg gctgttcatc gccgtgcccg atgacaaggt ggtcaagaac cgcatccatc
     5641 tcgacttccg gcccgacgat cagcaggcgg aggtcgaccg cgtgatcggg ttgggcgccc
     5701 ggcatgtcga catcggtcag ggtgagcaga cctgggtggt gctggccgac ccggagggca
     5761 acgaattctg catcctcgcg gcgcacgatt gatgcgacgt ccgcttctgt tgtgtgcggc
     5821 ggcactgctt gtcggcggat gcgccacggc ggtgccgatc gaacctgtat ccaccaccga
     5881 gccgacggcc cctccggccc cgctcacgat cggcccggcg gccaacgata cctacggcgg
     5941 atggattccc gacggccaga cgctcagccc gttcgacgtg tcgaatcccg ctttggccca
     6001 actggatcca gcgctactca acgccgtcca ggcggcggcc aacgccgcgc gcgggagcag
     6061 ggcatcgacc tgaggataaa ttcggggtgg cggtcacggg ggttccagca gcagttgttc
     6121 gacgacgccg cccgcaccta cggcagtgtg gacatcgcga agcaattcgt cgcgtcaccc
     6181 gatgtatcga aacacgttgt gggccaagcg gtggacgtcg cacccgttga ggctgacaag
     6241 tggctgatcc gcaacggaac ccagttcggt ctgtgtcaga tctatgcgaa cgagctctgg
     6301 cacttcgaac tcgccgccga cgcgcagggc aactgcccgc cgctgcgtcc taacgcagca
     6361 ggctgagcaa ttaagcccgc gccgggaatg ccttgcggga cagcaccact acggcgaggc
     6421 ccagcccgat cagcgacgca cccacccagg gcagcgatga caaccccgcc gcatgaagca
     6481 gaccggcgcc aatggccgct ccaccggcga tgcctgcgtt ggctgtcgcg ttcacccacg
     6541 caccggccag ttcgggcgat gcggcgtggg tgcgcaccgc gcacgtctgg tagatcgacg
     6601 gcaggccccc gaatgccccg ttccagacga ccgttgccac aatcactgcc acgagtgtcg
     6661 gccaggtgcc gccgagcgcg acgaccgcgc cgatcactat ggccagcagg accagggcgg
     6721 ttcgccttgg gcctcggtcg agattgcggc cggcccacca cacggcgagc aggccaccgg
     6781 caccgcacgc gagcagaatc gggccgacga aggtcgggct gaccccacta gacagcagca
     6841 acacggtgac aaacgtgtac accgtgaatt gcccaagaaa cgccaacaaa ttcgacgccg
     6901 atatcgcgac gagggcgccg cggccggctc caacggaact acctgacagc ggc
//
LOCUS       DASRJA010000011         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3381100,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000011 DASRJA010000000
VERSION     DASRJA010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3381100"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFC01_00545"
     CDS             <1..399
                     /locus_tag="VFC01_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HZN78166.1"
                     /translation="GADPNTHTKLGRGGLADIEWTVQLLQLRHAHKVPALHSTSTLDT
                     LDAIGRAELIAEGDVELLRQAWLTATRARNALVLVRGKPTDQLPGPGRQLNAVALAAG
                     WDSDDGGEFLDNYLRVTRRAKAVVRKVFGG"
     gene            410..1045
                     /locus_tag="VFC01_00550"
     CDS             410..1045
                     /locus_tag="VFC01_00550"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN78167.1"
                     /translation="MSMDFGFDVISAEEYDRQRVLYEPLTDAIRKLIGAGIHTDVDEA
                     TAREAQKAIEAVTAMLEGKQRTATTTLRHEATGRPLAWANPAVGVRNAIAPPMLIHHE
                     TGGRAWSEFTLSGAYEGPPGWVHGGICALVLDHILGEAASEGLTQPKFTGTITLRYLR
                     GTPLGPLRAEAFIERIEGVKTFARGYLSDAQGTTVEAEGVFIRPAWARDAG"
     gene            1042..1902
                     /locus_tag="VFC01_00555"
     CDS             1042..1902
                     /locus_tag="VFC01_00555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZN78168.1"
                     /translation="MKYYVSTAFLETSEVIEIAKAADDLGYDGMGIPDHVINLETLTT
                     PYPYTKDGKRRWEAFTDWPDPWVMIGAIALVTTRLHFVTTVYLPAMRDPYSAAKSIGT
                     AAYLAEGRLELGIGVGWCEEEFTLMGQRFDRRGKRTDEMLELMKALWQPGWTQFEGEF
                     YSAPRLEMEPTPPHIPIYVGGLSDIALRRAARNDGWIGDLITTDRAIERVDRLRELRA
                     ENGLSMDDFAILTPLTDAFAPEHYDRAEASGITGIITMPWMFYTGPESTLAEKIDGMR
                     KFRKDLALDR"
     gene            complement(1891..>2994)
                     /locus_tag="VFC01_00560"
     CDS             complement(1891..>2994)
                     /locus_tag="VFC01_00560"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZN78169.1"
                     /translation="SFVDETAVSVIRTADNAISRIPLAFRPEGVVVGPGGRHLYVADP
                     AGHRVAVVDTISGTTAFVEVGSRPFHMAVLDADVYVANNQGGTVSVIDTVDNVVVHTI
                     GLGGHPYAVASARGRVFVSDYGFYGGQSTHRVSMISAACGRVMDVLPVGHYPTGVAVA
                     PDDSRVYVTNNDGAYAGSHPGTTTVIDPVTGTVIDTLGVGGCAVALSDNGDHVYLASS
                     GYDATFTSKLSIVDIPSGRISAVPIQGTPNALAVNGDRVYVTDTWHSSIIQITARDVL
                     VVDTDAANDPPTLTITELETHLFQVSADDPDNDEVTVTATQPFSGTIKVLGGGLFQYT
                     PNARATEGFVDHFAITADDGHGGVVTKTVALTI"
BASE COUNT          565 a          926 c         1009 g          494 t
ORIGIN      
        1 ggcgccgatc cgaacacaca caccaagctg ggccgcggcg ggctcgccga catcgagtgg
       61 acggtgcagc tgcttcaact ccggcatgcc cacaaagtgc ctgcgctgca cagcacttcg
      121 acgctggaca cactcgacgc aatcggcagg gccgagctga tcgccgaggg cgacgtcgag
      181 ctgctacggc aggcgtggtt gacggcgacc agggcgcgca acgcccttgt gctggtgcgc
      241 ggcaagccaa ccgatcagct gcccggcccc ggccgccagc tcaatgctgt cgcgttggca
      301 gcgggctggg actccgacga cggcggcgag ttcctggaca actatctgcg ggtgacccgc
      361 cgggcaaagg cggttgtacg aaaggtcttc ggaggatgag gcacagcgga tgagcatgga
      421 cttcgggttc gacgtcatca gcgcggagga gtacgaccgt caacgcgtgc tctacgagcc
      481 gttgacagat gcgatacgca aactcatcgg cgcgggtatc cacaccgacg tcgatgaagc
      541 gaccgcgcgc gaggcacaga aggcaatcga ggcggttacc gcgatgctcg agggcaagca
      601 acgcaccgcg acgaccacac tgcgtcacga ggccaccgga cggcccctgg cgtgggcgaa
      661 tccggctgtc ggagtgcgca atgcgattgc gccgcccatg ctcatccacc acgaaaccgg
      721 tgggcgggcc tggagcgaat tcacccttag cggcgcctat gaggggccac cgggctgggt
      781 gcacggcggc atctgcgcgc tggtgctcga ccatatcctc ggtgaggcgg ccagcgaggg
      841 gctgacgcag ccgaagttca ccggcaccat cacactgcgc tacctgcgtg gcacacctct
      901 gggcccgctg cgtgccgagg cgttcatcga gcgaatcgaa ggtgtgaaaa cctttgcgcg
      961 cggctatctg agcgacgcgc agggcaccac cgtcgaggcc gagggcgtgt tcatcaggcc
     1021 ggcctgggcc agggacgccg gatgaaatat tacgtcagca cggcgttcct cgagaccagc
     1081 gaggtcatcg aaatcgccaa ggccgccgat gatctcggct atgacggaat gggtatcccg
     1141 gaccacgtca tcaacctcga gacgcttacc acaccctatc cgtacaccaa agacggtaag
     1201 cggcgctggg aggcgttcac cgactggccc gacccgtggg tgatgatcgg cgccatagcg
     1261 ctggtcacca cgcggctgca cttcgtgacg acggtgtacc tgcccgccat gcgcgacccg
     1321 tactctgccg cgaaatccat tggtactgct gcatatctgg ccgagggccg gttggagctt
     1381 ggtatcggcg tcggctggtg cgaggaagag ttcacgttga tgggtcagcg cttcgaccgc
     1441 cgcgggaagc gcaccgacga gatgctcgag ctgatgaagg cgctctggca gcccgggtgg
     1501 acccagttcg agggagagtt ctattcggcg ccgcgactgg agatggagcc cacgccgccg
     1561 cacattccga tctatgtcgg tggcctgagc gacattgcgt tgcgccgcgc agcacgcaac
     1621 gacggttgga tcggcgacct gatcaccacc gacagggcga tcgaacgcgt cgatcgactg
     1681 cgcgaattgc gcgcggaaaa cggcttgtcg atggacgatt tcgcgatcct gaccccgttg
     1741 accgatgcat tcgccccgga gcactacgac cgggccgagg cgagcggtat caccgggatc
     1801 atcacgatgc cgtggatgtt ctacaccggg cccgagtcga cgctcgccga gaagatcgac
     1861 gggatgcgca agttccgcaa ggatctggcg ctagatcgtt agggcgacgg tcttggtcac
     1921 gacgccgccg tgaccgtcgt cggcggtgat ggcgaagtga tcgacgaatc cttctgtcgc
     1981 ccttgcgttg ggtgtgtact ggaataggcc gccaccgagc acctttatag tgccgctgaa
     2041 cggctgcgtt gccgtgacgg tcacctcgtc gttgtcggga tcgtctgcgc tgacctggaa
     2101 taggtgagtc tcgagctcgg tgatggtcaa cgtcggcgga tcgttggccg catcggtgtc
     2161 gacgacgagc acgtcgcgtg cggtgatctg gatgatcgac gaatgccagg tgtcggtgac
     2221 atagacccga tcgccgttga cagccaaggc attcggcgtc ccctggatgg ggacagcgga
     2281 gattcggccg gaagggatgt cgacgatcga gagcttgctc gtgaacgtcg cgtcatagcc
     2341 gctgctcgcc aggtagacgt gatcgccgtt gtcgctgagc gcgaccgcac aaccaccgac
     2401 gcccagtgtg tcgatcacgg tgccggtaac cgggtcgatg acggttgtgg tgccgggatg
     2461 agagccggcg taggcgccgt cgttgttggt cacgtacacc cggctgtcat ccggcgcgac
     2521 agcaacaccg gtggggtagt ggccgacggg caacacgtcc ataacccggc cacacgcggc
     2581 gctgatcatc gacacccgat gggtggactg gccgccgtag aagccgtagt cggagacgaa
     2641 gacccggccg cgagccgatg ccaccgcgta agggtggccg cccagcccga tcgtgtgaac
     2701 cacgacgttg tcgacggtgt cgatcacgct gacagtgccg ccctgattgt tggccacata
     2761 cacatcggcg tcgagaaccg ccatgtggaa tggacgagaa cccacctcga cgaacgccgt
     2821 agtgcccgag atcgtgtcga ctaccgcaac ccggtgtcct gctgggtccg cgacgtagag
     2881 atggcgaccg ccagggccga cgaccacgcc ttccggacgg aacgccaagg gaattcgcga
     2941 gatggcattg tcggccgttc ggataaccga aaccgccgtt tcgtcgacga agct
//
LOCUS       DASRJA010000012         4982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2245577,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000012 DASRJA010000000
VERSION     DASRJA010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2245577"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="VFC01_00565"
     CDS             complement(<1..1379)
                     /locus_tag="VFC01_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN78170.1"
                     /translation="MTTTAEHLRNTLDGRWRDVKNRMREELSNEVFRPHYTPNTVIAR
                     TKVAEQMKIMAAHGAAEDGFKKEHGGNGDVAAAVTRIEMLAMSDLSLMVKAGVQWGLF
                     GGAIENLGTERHHQAYVPRIISLDLLGCFAMTETGHGSDVQSIETTATYDASTQEFVI
                     DSPTRTSRKDYIGGAAESATVAAVFAQLITPDDEGHGVHCFVVPIRDEDGNDLPGVTT
                     SDCHYKGGLPGVDNGRIQFDHVRIPRENLLNKYADVAEDGSYSSPIENPNRRFFTMLG
                     TLIRGRVTVGGSAGSAARVALDIATRYALERRQFKAPEDDHEVLLMDYLVHQRRLFPL
                     IARSYALQFAQNELVAKTHEMQTADDPDPEEQRELESRAAGLKAANTWHATRAIQEAR
                     EACGGAGYLAENRLIALKADTDVFTTFEGDNHVLIQLVAKELLTAYADDIKGMSPVEW
                     VRFAANFAG"
     gene            1480..2208
                     /locus_tag="VFC01_00570"
     CDS             1480..2208
                     /locus_tag="VFC01_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZN78171.1"
                     /translation="MTESQSSTPQSWRPHDFDHQHGDVSGGWLRAATFGAMDGLVSNT
                     ALIAGVGAAADAHTVVISGFAGLVAGAFSMALGEFASVTTANEQIESEVTVEKRAFRL
                     HPQAEQAELTDMFVNIGLSPETAAKAADEMHQDESRAVNFHLTQELGVDPEDKPSPYV
                     AAGSSFIMFAVGAIVPLIPYLLGFRSLWLGLAFGGVGLLIAGGVAARFTKKPLWFASL
                     RQLLFGTIAIAATYLVGHLVGTVV"
     gene            complement(2205..3416)
                     /locus_tag="VFC01_00575"
     CDS             complement(2205..3416)
                     /locus_tag="VFC01_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN78172.1"
                     /translation="MWRQPKAVWAVAFASVVAFMGIGLVDPILKPIADNLNASPSQVS
                     LLFTSYMAVMGVAMLITGVVASQIGPKRTLLLGLLIIIAGAGLAGMSDTVLEIVGWRA
                     LWGLGNALFIATALATIVSSARGSVAQAIILYEAALGLGIAVGPLVGGVLGSMSWRGP
                     FFGVSVLMAFAVIVTAFLLPSTPRAERATTLADPFRALRHRGLLGVAITALLYNFGFF
                     TLLAFTPFPLDMTAHQIGLIFFGWGLALAFTSVVVAPRLQHRFGTVPTLLVNLLAMTA
                     TLTAMAVGTENKTVLATCVVVAGLFIGINNTLITETVMKAAPVERGVASAAYSFVRFS
                     GAALAPWLAGVLGERISVHLPYWVGAAAVLLGAGVLFAARQHLVGIDVEDSELDEITE
                     QATAITVGSDS"
     gene            complement(3426..3878)
                     /locus_tag="VFC01_00580"
     CDS             complement(3426..3878)
                     /locus_tag="VFC01_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN78173.1"
                     /translation="MTPTLGADLLSVVARINRLATQRVRLPLGFAQARLLSTIEDQGA
                     ARISDLAALDHCSQPTMTTQVRRLEDAGLVSRTVDPDDARAVLIRITQKGVETLRQVR
                     VDRGAAIDPYLERLDAADRQTLSDAVRVLRKLLEDAAAPIHQVTTEKE"
     gene            3988..>4982
                     /locus_tag="VFC01_00585"
     CDS             3988..>4982
                     /locus_tag="VFC01_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HZN78174.1"
                     /translation="MTATEPSPPRRAGSLQPVELAQASVMAALCAATAIIAVVVPFAA
                     GVSLLGTVPMGLLAYRYRLRVLIAATVAGAVIAFLIAGMGGFMTVVNCAYIGGLTGIV
                     KRRGRGILTVFAAALLAGALFGVATVAALAILSRLRHLIFDSVTANINGLAAVIARIP
                     DMEPVAERLKHDFATALHYWPFLFFGSSVLSIVFVTFIGWWALSRVMRRLLGIPDVHK
                     LESSTDIGPVAPVPARLHDVVFRYPKADHDALGPVSLSVEPGEHLAVTGANGSGKTTL
                     MLMLAGRQPTSGTIERPGAVGLGKLGGAAVIMQHPESQVLGTRVADDVVWGLPPG"
BASE COUNT          808 a         1631 c         1666 g          877 t
ORIGIN      
        1 tcgccggcga agttggcggc gaaccgcacc cactccacgg ggctcatgcc cttgatgtca
       61 tcggcgtagg cggtgagcag ttctttcgcc acaagctgga tcagcacgtg gttgtcgccc
      121 tcgaatgtgg tgaacacgtc ggtatcggcc ttcaacgcga tgagcctgtt ctcggcgaga
      181 tagcctgcgc cgccgcaggc ttcgcgcgcc tcctggatgg cgcgagtggc gtgccaggtg
      241 ttggccgcct tcaggcctgc ggctcgtgac tcgagctccc gctgctcttc gggatcgggg
      301 tcgtcggcgg tctgcatctc atgcgtcttg gcgaccagtt cgttctgcgc gaactgcaac
      361 gcgtaggacc gcgcgatgag cgggaacaac cggcgctgat gcaccagata gtccatcagc
      421 agcacctcgt ggtcgtcctc aggtgccttg aactgcctgc gctccaacgc atatcgcgtc
      481 gcgatgtcga gcgccacccg cgccgcggaa ccggcgctgc cgccgacggt gacccggccg
      541 cggatcaggg tgccgagcat ggtgaagaag cgtcggttcg gattctcgat cggcgaactg
      601 tacgaaccgt cctcggccac gtcggcgtat ttgttcagca ggttctcccg cgggatgcgg
      661 acatggtcga actggatgcg accgttgtcc acaccgggca gtccgccttt gtagtggcag
      721 tcgctggtcg tcacccccgg caggtcgttg ccgtcctcgt cccgaatcgg caccacgaag
      781 cagtgcaccc cgtggccctc gtcgtccggc gtgatcagct gagcgaatac tgctgcgacg
      841 gtggcacttt cagcagcccc gccgatgtag tccttacgcg aggtgcgagt gggggagtcg
      901 atgacgaact cctgggtcga cgcgtcgtag gtggcggtcg tctcgatcga ctgcacgtcg
      961 ctgccgtggc cggtctcggt catcgcaaag caacccaaca ggtccaggct gatgatccgc
     1021 ggcacgtacg cctggtggtg gcgctcggtg ccgaggtttt cgatggcccc gccgaaaagc
     1081 ccccactgca cgcctgcctt gaccatgagc gacagatcgg acatcgccag catctcgatc
     1141 cgggtgaccg ccgcggcgac gtcgccgttg ccgccgtgct ccttcttgaa gccgtcctcg
     1201 gcggcgccgt gggccgccat gatcttcatc tgctctgcca ccttggtgcg ggcgatgacg
     1261 gtgttaggcg tgtaatgcgg gcggaagacc tcgttggaga gttcctctcg catcctgttc
     1321 ttcacgtcgc gccaacggcc gtccagcgtg ttgcgtagat gctccgcagt ggtggtcata
     1381 accagacggt agcccttcgg cggtgcgtgg aaactgcgat gtggcaaacc taggcaaacc
     1441 tagctagacc agcagaaacc catgccgttg aatcgaggca tgaccgagtc acagtcatcg
     1501 accccgcagt cgtggcggcc gcacgacttc gatcatcagc acggcgacgt cagcggcggc
     1561 tggttgcgcg ccgcgacgtt cggcgcgatg gacggcctcg tcagcaacac cgcgctgatc
     1621 gcgggtgtgg gcgcggcggc agatgcacac accgtggtga tcagtggctt cgccggcctg
     1681 gtcgccggcg cattctcgat ggcgttgggc gagttcgcat cggtgaccac ggccaacgag
     1741 cagatcgaat ccgaagtgac cgtcgagaaa cgggcctttc gactgcatcc gcaggccgag
     1801 caggccgagc tcaccgacat gttcgtcaac atcggcttat cgccggagac cgcagccaag
     1861 gcggccgacg agatgcacca ggacgagagc cgtgcggtga actttcatct gacccaagag
     1921 ctgggcgtcg acccggaaga caagccgtcg ccctacgtgg cggccgggtc gagcttcatc
     1981 atgttcgccg tcggcgccat cgtcccgctg atcccgtacc tgctcggctt ccggtcgctg
     2041 tggctcggcc tggccttcgg cggcgtcggc ctactgatcg ccgggggagt cgcggctcgg
     2101 ttcaccaaga agccgctgtg gtttgcgtcc ctgcgtcagc tgctgttcgg gacgatcgcg
     2161 atcgccgcga cgtatctcgt cggccacctc gtggggaccg tcgtctagct gtcgctgccg
     2221 acggtgatcg ccgttgcctg ctcggtgatc tcgtcgagtt cggagtcctc gacgtcgatg
     2281 ccgaccagat gctgacgcgc ggcgaaaagc acaccggcgc ccagcagtac ggccgcggcg
     2341 cccacccagt acggcaggtg cacgctgatc cgctcgccga gcacaccggc cagccacggc
     2401 gccagcgcgg cgccggagaa tcgtacgaag ctgtaggcgg ccgaggccac tccacgctcg
     2461 accggcgccg ccttcatgac ggtctcggtg atcagcgtgt tgttgatgcc gatgaataga
     2521 ccggcgacca ccacgcacgt cgccaagacg gtcttgttct ccgtgcccac ggccatcgcc
     2581 gtcaacgtcg ccgtcatcgc cagcaggttg accaatagcg ttggcacggt gccgaagcga
     2641 tgctgcagcc gcggcgcgac gaccacagag gtgaacgcca gcgccagccc ccagccgaag
     2701 aagatcagcc cgatctggtg ggcggtcatg tcgagtggga acggcgtgaa cgccagcagc
     2761 gtgaagaagc caaagttgta cagcagcgcg gtgattgcga cgccgagcag gccgcggtgg
     2821 cgcagcgctc ggaacgggtc ggccagcgtg gtggcacgct cggcgcgcgg tgtggacggc
     2881 agcaggaacg ccgtgacgat gaccgcgaac gccatcagca ccgagacccc gaaaaatggt
     2941 ccacgccatg acatcgagcc gaggacgcca ccgaccaacg ggccgaccgc gatgccgagg
     3001 cccagcgccg cttcatagag gatgatcgcc tgggcgaccg atccgcgcgc ggagctgacg
     3061 atcgtcgcca acgcggtggc gatgaacaac gcgttgccca gaccccaaag tgcacgccag
     3121 ccaacgattt ccagcacggt gtcggacatt ccggccaagc ccgcgccggc gatgatgatc
     3181 aacaatccca acagcagtgt gcgtttgggc ccgatctggc tggccaccac accggtgatc
     3241 agcatggcca cgcccatcac cgccatatag ctggtgaaca acagcgacac ctgcgacggc
     3301 gacgcgttca ggttgtcggc gatcggcttg agaatagggt cgacgagacc gatgcccatg
     3361 aacgcgacga cggacgcgaa agcaacggcc caaacagcct tgggttggcg ccacatacgg
     3421 tgcggctact ccttttcggt tgttacttgg tggatggggg cggccgcatc ctcgagaagc
     3481 ttgcgcagca cgcgcacggc gtcgctgagc gtttgccggt cggcggcatc tagccgctca
     3541 agatacgggt cgatggccgc accgcggtcg acacgcactt gccgcagggt ctcgacgcct
     3601 ttctgggtga tgcgaatcag taccgcccgc gcatcgtcgg ggtcaacggt gcgcgatacc
     3661 aggcccgcgt cttcgagccg gcggacctgg gtggtcatcg tcggctgcga gcagtggtcg
     3721 agtgcggcca ggtcggatat ccgggccgca ccttgatcct cgatcgtgga caacagccgc
     3781 gcttgggcga atcccagcgg caggcggacc cgttgcgtgg ccagtcgatt gatccgggcc
     3841 actacggata gcaggtcggc tccgagcgtc ggggtcatga gttccatgat tgcataggtt
     3901 tactatgcag ttcaaacgca cgcgccggtg gcggcggtca cagcgtgggc gcgcgggcgc
     3961 tcacgcggcg gctggcagaa tcgggagatg accgcgaccg agccgagccc cccgcggcgg
     4021 gccggctcgc ttcagccggt cgagcttgca caagcctcgg tgatggcggc gttgtgtgcg
     4081 gccaccgcga tcatcgcagt cgtcgtcccg ttcgctgccg gagtgtcgct gctcggcact
     4141 gtgccgatgg gtctgctcgc ctaccggtat cggcttcggg tgctgatcgc ggcgactgtc
     4201 gccggtgccg tcatcgcatt tctcatcgcc ggcatgggcg gattcatgac cgtggtcaac
     4261 tgtgcctaca tcggcggtct gaccggcatc gtgaaacgtc gcggccgcgg gatcctcacg
     4321 gtgttcgctg cggcgctcct cgcgggcgcg ttatttgggg tcgcgacggt ggcggcgctt
     4381 gcaatcctgt cgcggctgcg ccacctgatc ttcgattcgg tgaccgccaa catcaacgga
     4441 ctcgccgcgg tgatcgcccg cattcccgat atggagcccg tcgccgagcg gctcaagcac
     4501 gacttcgcca ccgcgctgca ttactggccg ttcctcttct tcggctcgtc ggtgttgtcc
     4561 atcgtcttcg tcaccttcat cggttggtgg gcgctgtcgc gagtcatgcg ccgcctgctg
     4621 gggattcccg atgtgcacaa gctggagtcg tcgaccgata ttggaccggt ggcgccggtg
     4681 ccggctcggc tgcacgacgt cgtcttccgc tacccgaagg ccgaccacga cgcgctgggc
     4741 ccggtgtcgc tgagcgtgga acctggcgag cacctggcgg tgacgggcgc caacggctcg
     4801 ggcaagacca cactgatgct gatgctcgcc gggcgccaac ccacgtccgg aaccatcgag
     4861 cgccccggcg ctgtggggct cggcaagctc gggggcgccg cggtgatcat gcaacatccg
     4921 gagagtcagg tactgggcac gcgcgtcgcc gacgacgtgg tgtggggcct gccgcccggc
     4981 ca
//
LOCUS       DASRJA010000013         3473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1658310,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000013 DASRJA010000000
VERSION     DASRJA010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658310"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="VFC01_00590"
     CDS             <1..1433
                     /locus_tag="VFC01_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78175.1"
                     /translation="QGAPIFAWSGDDHLTGSTGKDLFVFAQPIGDDIIYSFDASQDQI
                     DLIGYAGFASFDDVKNHLTADANGNAVITLADGQSITLDGVAATSLSADNFVFDQTPV
                     TNNAGTMTVGDGAMLPLSGIINNTGTIALESSGNETHLELIQYGVMLEGGGQVVLSDS
                     SENIISGTLPSVTLTNVDNTISGAGQLGAGQLTLVNHGTIIATGSNALIIDTGANAVI
                     NSGTLEAAGSGGLVIYSDVENSGLLWANGSDLTVTGNVSGDGSAKLDGVATLEFGAAV
                     DQDISFDASAAGTLKLDNADSFVGVVSGFDGNDQFDFSDIDFGAEVTLTYTANQEGTG
                     GTLTVSDGTDTAHIDLVGEYSEEGFQTAADSGAGTLVTYTPSDAAANDVVTGGVGTDD
                     VLSGGVGADTFQFAPSFGHDTIVNFTEGEDKIDFDPAIFATIDDILAHTQQVGSDTVI
                     TVDQASSVTLTNVDMSTLSASDFIHH"
     gene            1502..3262
                     /locus_tag="VFC01_00595"
     CDS             1502..3262
                     /locus_tag="VFC01_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HZN78176.1"
                     /translation="MSRSTTKSPTTRDTPSAELAAALGSCRGAFVATGLISGMSNILM
                     LTGAIFMLEVYDRVLPSRSVPTLIGLAMLAGGLYVALGLLDLIRGRILTRIGISLDEA
                     LSGRIYQTIVRLPSIVGLRNDGAGPMRDLDSVRSFLGSLGPVALFDLPWMPVYLGMCF
                     AFHFWIGMTATVGALILVVVTIFTEWFSRKPTMTATQLAQSRNAMIESSRRNSEALVT
                     MGMVPHLTGRWSRVNQEFLASNRRASDAAGGFGAISKVLRLMLQSAVLGVGAYLVIYQ
                     QASAGIIIAGAIISARALAPVDIAIAHWRGWVSARQSWDRLKKLLATIPPDSEPMPLR
                     APQTSLVVENASVAPPGTRKPAVRDMSLKLQRGSGLGVIGPSASGKSSLARMLVGYWA
                     PANGKVCLDGASLDQWAPDILGRHIGYLPQHVELLNGNVAQNISRFEPEADPEAIRAA
                     AETAGAHQMIVSLEDGYSRQIGERGSALSAGQAQRIALARALYRDPFLVVLDEPNSNL
                     DAEGEAALTQAILSIRARGGIVVVIAHRPSAIAGVDLVLAMKEGRAVAFGPKDEVLAK
                     VLQREPSPRPLKLVSEAGSA"
BASE COUNT          646 a         1053 c         1114 g          660 t
ORIGIN      
        1 cgcagggcgc gccgatcttt gcctggtcgg gcgacgatca cctgaccgga tcgaccggca
       61 aggacctgtt cgttttcgcg cagccgattg gcgacgacat catctacagc ttcgatgcca
      121 gccaagacca gatcgatctc atcggctatg ctgggtttgc cagcttcgat gatgtgaaaa
      181 atcatctgac cgcggatgcc aatggcaatg cggtgatcac gctggcagac gggcagtcga
      241 tcacgctcga cggcgtagcc gccacttctc tcagtgcaga caacttcgta tttgatcaga
      301 cgccggtcac aaacaacgcc gggacgatga ccgtcggcga cggcgctatg ctgccgctga
      361 gcggcatcat caacaacacc ggaaccatcg cgctcgagtc gagtggaaat gagacgcatc
      421 tggagctcat tcagtacggc gtcatgctcg agggtggcgg tcaggtcgtt ctgtcggaca
      481 gcagcgagaa tattatctcc ggtacccttc ccagtgtcac gctcaccaat gtggacaaca
      541 cgatctccgg tgccggccag ctgggagctg ggcagctgac gctggtcaac cacggcacca
      601 tcatcgccac gggaagcaac gcgctcatta tcgataccgg cgccaacgcc gtgatcaatt
      661 ccggcacgct ggaggcggcc ggcagcggcg gactggtgat atacagtgac gtagagaatt
      721 ctgggctgct ttgggcgaac ggcagcgact tgacggtgac cggcaacgtg agtggtgatg
      781 ggtcggcaaa gctcgacggt gttgccacgc tggagtttgg cgctgcggtc gatcaagaca
      841 tctcgttcga cgccagcgct gccggcacgt tgaagcttga caacgcggac agcttcgtcg
      901 gcgtcgtctc aggctttgac ggcaacgatc aattcgattt ctccgacatc gactttgggg
      961 ccgaggtgac gctcacctat actgcgaacc aggaaggcac cggcggcacg ctgaccgtga
     1021 gcgacggcac ggatacggcc cacattgatc tggtcggcga gtattcagaa gaggggttcc
     1081 agaccgcggc agatagcggc gcggggacac tcgtcaccta cacgccgtcc gacgcagcgg
     1141 caaatgatgt ggtaaccggc ggcgtaggca ccgacgacgt tttgtcgggc ggagtcggcg
     1201 ccgacacctt ccagtttgca cccagcttcg gtcacgacac gatcgtcaat tttacggagg
     1261 gcgaggacaa gatagacttt gatccggcga tcttcgcaac tatcgatgac atactggctc
     1321 acacacaaca ggtgggcagc gatacggtca tcaccgttga ccaagccagc agcgtcacct
     1381 tgacaaatgt ggacatgagc acattgtcgg caagcgattt catccaccac tgatccgcgg
     1441 gcgttgcggt ccgtagtatt cgtcgggctt tggtcacgat gcttcagtat agggttggct
     1501 tatgagccgc tccaccacca agtctccgac tacgcgcgac accccttccg ccgagctggc
     1561 cgcggcgctg ggatcctgcc gcggcgcgtt cgtcgctacc ggcctgatca gcggcatgag
     1621 caatattctc atgctcaccg gcgcgatctt catgctcgag gtctacgacc gagtgctgcc
     1681 gagccgcagc gtgccgacgc tgattgggct cgccatgctc gcgggcggcc tctatgtggc
     1741 gctcgggctg ctcgacctga tccgagggcg catcctcacc cggatcggca tcagcctcga
     1801 cgaggcgctg agcggacgaa tttatcagac gatcgtgcgc ctgccgtcga tcgtcgggct
     1861 acgcaacgac ggcgccgggc cgatgcgaga cctggacagc gtccggtcgt ttctgggcag
     1921 ccttgggccg gtggcgctat tcgatctgcc ttggatgccg gtctatctcg gcatgtgctt
     1981 cgccttccat ttctggatcg gcatgaccgc gacggtcgga gcattaattt tggtcgtggt
     2041 gaccatcttc accgaatggt tctcgcgcaa gccgacaatg acggcgaccc agctggcgca
     2101 gtcacgcaac gcgatgatcg agtcgagccg gcgcaattcc gaggctctgg tcaccatggg
     2161 catggtgccc catctcaccg ggcgttggag cagggtcaat caggaatttc ttgcgagcaa
     2221 ccggcgtgcg agtgacgcgg cggggggatt cggcgcaatc tcgaaggtgc tgcggctgat
     2281 gctgcaatcc gccgtgctcg gggtgggcgc ctatcttgtc atctatcagc aagcgtcggc
     2341 aggaattatc atcgcgggcg cgatcatcag cgctcgcgca ttggcgccgg tcgatattgc
     2401 gattgcccat tggcgaggct gggtgtcggc gcggcaaagt tgggatcgac tgaaaaaatt
     2461 gctggcgacg atcccgccgg acagcgagcc gatgccgctg cgcgcgccgc agacgagcct
     2521 ggtggtcgaa aatgccagtg ttgcaccgcc gggcacgcgc aaacccgccg tgcgcgacat
     2581 gtctttgaag ctgcagcgcg gaagcggcct cggcgtaatt gggccgagcg cttctggcaa
     2641 gtcgtcgctc gcgcgaatgc tggtcggata ttgggcacct gcgaacggca aggtttgcct
     2701 cgacggcgcg tcactggatc agtgggctcc ggatattctg ggacgccata tcggctatct
     2761 gccgcaacac gtcgaactcc tcaacggcaa cgtggcgcaa aacatcagcc ggttcgaacc
     2821 cgaggcggat cccgaggcga tacgggcggc ggcggagacc gctggcgccc atcagatgat
     2881 tgtttctctg gaggacggat acagccggca gatcggcgag cgaggttcgg cgctgtcggc
     2941 ggggcaagca cagcgcatag cgctggcccg cgcgctctat cgcgacccgt tcctggtggt
     3001 tctcgatgag ccgaattcca atctcgatgc cgaaggcgag gcggcgctga cccaagccat
     3061 cctcagcatc cgtgcccgcg gcggcatcgt ggtcgtgatt gctcaccgcc caagcgcaat
     3121 cgctggggtg gacctcgttc tggcgatgaa ggaagggcga gcggttgcct tcggtccgaa
     3181 ggatgaggtg cttgcgaagg tgctccagcg cgaaccgtca ccgcgccccc tcaagctcgt
     3241 ttctgaagcc gggagtgcct agcgtgcgaa ctcgggcctc accaacgcga cgatccattc
     3301 ggcgcaacat tctcgccggt cttatagttg tgattattct cgccggcggc gtcggcgggt
     3361 gggccgccac cgcgcagatt tccggcgcgc tcatcgcgcc cggctccctc gtcgtcgact
     3421 cgaacgtgaa aaaggtgcag catccgaccg gcggcgtcgt cggcgaggtg cgc
//
LOCUS       DASRJA010000014        12493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3938922,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000014 DASRJA010000000
VERSION     DASRJA010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3938922"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFC01_00600"
     CDS             <1..531
                     /locus_tag="VFC01_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN78177.1"
                     /translation="RSLAVLEPSLAAGTPIVGLEPSCTAALRHDAPALLPDNPLAAAT
                     ATATRTFAEFLNESGWQPPQVGGEALVQTHCHQHAVVGFDADRSVMKAAGIDATVPDS
                     GCCGLAGNFGFERGHYEVSKAAGERVLLPAVRDAAPTTAIVADGFSCRTQITHGTSRQ
                     PVHLAQLLAAALRQGR"
     gene            complement(519..1706)
                     /locus_tag="VFC01_00605"
     CDS             complement(519..1706)
                     /locus_tag="VFC01_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006900391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZN78178.1"
                     /translation="MGVSAAEQAVLDRIDEGSLVELTTSLVRAAGQNPPGDEAATVRV
                     LSTAAAELGLDVVETAVEPGRDNVRITLEGGTGPGLLLLGHTDVVPVGDGWTVDPFGG
                     AVHDGRIYGRGASDMKGGLAASLSALAALRGVALSGPVELTALVDEEETGKGIRAFVE
                     SVRRPFLGCVTAEPTDLQTIIGARGASYLRIEVHGKACHAGNPDDGANAIYGAAAVVA
                     EIERLHAELATHPHPLLGPATWSVGQIQGGTGGSIVPAECVVVADRRLLPGESTDAVL
                     DELRERVTGLRLAERGLTVDLVMPMEMPAFQTPADSELARVTDAALADAGGPGQPLGG
                     WTAACDGGFVARDLGVPVVVLGPGSATTQAHRADESVAIEELLVAARAYALTALRLLS
                     QRP"
     gene            complement(1711..2913)
                     /locus_tag="VFC01_00610"
     CDS             complement(1711..2913)
                     /locus_tag="VFC01_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006900392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZN78179.1"
                     /translation="MRSSTLPYAARATGLVGSVIDSSTSLLHKQTHDVVRFAMGSPAA
                     EAVPSEILAAIAAVELAEPHAYDYAATEGDPPLHAALLHALRGTSDETTADRLTITAG
                     GMQGLDLFCKIFVDPGDLVTVESPTYTNGSATALSYGATLLEIPVDDDGMDIDALEGL
                     VAAAGRTPKAIYTIPTFQNPSGVTLSLERRHRLLDLARAWGSMVIDDDPYGLLRFAGD
                     PLPTLRELAAGDPLVFSVRTFSKIVAPGLRVGWVDTVPELQPLLINAKQAMDTCTNLP
                     AQRLLTGFLTGGHLENHLVTQRAEYKRRNEAMHTALREHFGDIAHWTDPEGGFFLWVT
                     LENGVDTTELFERALAEGVAFIPGPAFSPSGRFTHALRLCFASTPPDRIYEGVARLRR
                     AVDLLAKG"
     gene            complement(2917..4083)
                     /locus_tag="VFC01_00615"
     CDS             complement(2917..4083)
                     /locus_tag="VFC01_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZN78180.1"
                     /translation="MTPDTAEYTERLRRVREAMAQRAFGALVVCDPANLFYLTGYNAW
                     SFYTPQCLLVPAEGELHLFARAQDANGATFTCNLPADQIHGYPEELVHRPDVHPFDWI
                     AAESRELVPAGELVGVEGDAHFFSPRGYLALDNGLPENRLVDSAELVNWVRLIKSPYE
                     VEQLRTAGTIAERAMRTALDGLRPGRRQCDVVAEILAAQASGTPEHGGDYPALWPLLP
                     TGDAAGTPHLTWTDRPFRNGEATTIELAGAFGRYHAPLARTVMLGDPPQRLTETAKIV
                     AEAMHATLEAIRPGVTGSAVHEAFDRVIRSHGLRKDSRIGYSIGIGYPPDWGERTVSL
                     RHGETTELAAGMAFHIILGIWMDDWGYELSEPVVVTATGVERLTDLPHELTIWR"
     gene            complement(4080..4802)
                     /locus_tag="VFC01_00620"
     CDS             complement(4080..4802)
                     /locus_tag="VFC01_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu racemase"
                     /protein_id="HZN78181.1"
                     /translation="MAVSPPLEQTGIGVVVPYDFALDRELWRWVPDDISLHLTRTPYV
                     PLAVTIEMVAHISDPSVVANGVSELKAVSPLVTAYACTSASFVGGLAGETALVAAMKQ
                     AGAPAAVTTSGALLAALRHLDITRIATATPYTADLTAGLSSYLAEAGIEVVAAAGLGL
                     TSDIWTVPYDVTAQLVRDTDRPDAEAVFISCTNLPTYDMIATLEAELGKPVLTANQVT
                     MWSALTVVGRKAVGPGQQLLAR"
     gene            4882..5859
                     /locus_tag="VFC01_00625"
     CDS             4882..5859
                     /locus_tag="VFC01_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008715411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HZN78182.1"
                     /translation="MPTRPVVAVLCERATDRPPGLDALDVDFRYCAADGLAEAVRGAR
                     GLLLWDFFSTAVRDVWADAGTVEWIHVTAAGVDTLLFDELRDSDVVVTNAHGVFDRPI
                     AEYVLGAVIAYAKDSRRSFDLQRRHQWQHRETRTVAGATALVVGTGGIGREIARLLRA
                     AGIEVRGAGRKARDDDPDFGKVVASGDLSAEVGWCDHLILAAPLTDDTRGLVDAAVLS
                     AMKPDAHLVNIARGPLVDEAALLAALTGGRIGGATLDVFTEEPLPADHPLWDAPNATI
                     TAHMSGDVVGWRDTLAAQYAENVRRWLAGEPLLNVVDKKLGYIPGGSAR"
     gene            5856..7259
                     /locus_tag="VFC01_00630"
     CDS             5856..7259
                     /locus_tag="VFC01_00630"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN78183.1"
                     /translation="MIPMAVDLVEAFRRKELSPVEATAAALEAIDRYDDQVNAFVLVD
                     AEGALAAAKESEARWHAGEPLGPGDGVPTSIKDALWTRGWPTLRGSTLIDKAGPWEED
                     APSVARLRETGAVIIGKTTTPEYSWKGVTDSFRHGITRNPWDPGTTAGGSSGGSAAAV
                     GLGMGPWSVGTDGGGSVRIPAGFTGTVALKPTYGLVAHYPASAFGSLSHVGPMTRSVR
                     DCAALLDVISGFDARDCTAMPTPTKSFLDGLDNGVAGLRIAFSPNFGFVRNDPEVDIA
                     VRAAVDVIASAGAEVDEVDPGFADPVEAFHVLWFSGEAHVLRAYGDAVDDRVDPGLRR
                     TAAIGAAYSAADYLDATAVRMELGVLMGRFHQTYDVLVTPTLPLPAFPAGQDVPDGWS
                     SPDWTSWTPYTYPFNLTQQPAMSVPCGFTSAGLPIGLQVVGPRHADALVLRVGQAYQS
                     ATDWHLRTPALLTEEVR"
     gene            7256..7756
                     /locus_tag="VFC01_00635"
     CDS             7256..7756
                     /locus_tag="VFC01_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HZN78184.1"
                     /translation="MSRYITVSLDKRSVSCVAVLLDNEAPRTCAAVWDALPLSAPVFH
                     GKYARNEIYTLLPPFAPSDPGKENTTITPIPGDLCWFSFDSDQLGNPAYGYENTTGTG
                     TTGAIVDLALFYGRNNLLINGDQGWVPGNVFGAVVEGLDEMAEACQDLWMGGVRGETL
                     SFSRRD"
     gene            7781..8593
                     /locus_tag="VFC01_00640"
     CDS             7781..8593
                     /locus_tag="VFC01_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN78185.1"
                     /translation="MGVRKATTPSAPVRGLVGRAGTTSLFDLHRWQPTGVAAQFVEHY
                     WSVSWDLRDPYESTVITFPAVHLTNEWGTDGSRHGHTLPAVLLHGVVERVFRTTIRGT
                     GTVVGARFHPGGFTARYGRDAGSLKGRVERAEELLPDAPRDVENCLAALESAIGSDAS
                     LDDTYVRLRKLVELIRDDDRLHRVEQVMEHSPWSARTTQRVFRRYVGVPVKWVLCRYR
                     LQQAALEIESRPGVDHADLAARLGWYDQAHFINDFRTMLGSTPGEYAASYGS"
     gene            8602..9981
                     /locus_tag="VFC01_00645"
     CDS             8602..9981
                     /locus_tag="VFC01_00645"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN78186.1"
                     /translation="MDSRELAFAGAAQQARMLADGAITAPELLELYLDRIARLDPQLR
                     SYRVVLADSARQEAGVAQDRLNAGERLPLLGVPIAIKDDVDVAGGVTTYGSAAHGPAC
                     TQDAEVVRRLREAGAVIVGKTNVPELMVWSFTETLAYGATRNPWNTDYTPGGSSGGSG
                     AAVAAGLAAMALGSDGMGSIRIPSTWCGLFGIKPQRDRVPLAPHDDAWNGLSVNGPIA
                     RTVEDAALFLDVTAPGNEFVAATKRPPGQLRIALSAKAPPGLVVRVGRAQRAAFDEAG
                     ALLRELGHEVVERDPDYPPAAVYGQALPRYFRGVYDDVKALPHPERLDARTRAFARIG
                     GLISDRRMRAIRGAESIAAERIQSIFDDVDVVITPGTAMGPSRIGAYQRRGAIPTLAL
                     VAARVPFQAMFNVTGQPAAVVPWDSDGNGVPTSIQLVGRPFDEATLLSLGAQIEQARP
                     WAHRRPPVS"
     gene            complement(9976..10470)
                     /locus_tag="VFC01_00650"
     CDS             complement(9976..10470)
                     /locus_tag="VFC01_00650"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HZN78187.1"
                     /translation="MTSELLHGLPPIIADGAHTLILGNMPSVMSLASQQYYANPRNAF
                     WRITGEIYGFDASALYDVRTATLTAHGIAVWDVLRSCRRIGSLDSAVEPDSMVANDFQ
                     RFFNRHPSITRVFFNGAAAEKNFNRLVRVMPDFQYRRLPSTSPAQTMRYEDKLAAWRI
                     IDES"
     gene            10513..11640
                     /locus_tag="VFC01_00655"
     CDS             10513..11640
                     /locus_tag="VFC01_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN78188.1"
                     /translation="MAEEFDLEASGLLDGLEGKARDERAELIPWLLERGITVEQIRGA
                     YAPALLGARKILGDDGTYVSAREASEKTGIDLDLLQRIQRAMGLPRVDDPDAAVHLRA
                     DAEAAAFAQKFLELGIDPDQLVQVSRILNEGLSRAAEVMRNAALVSVLNPSATELDIA
                     KNSEAFLSQAAPMLGPMIQEILRLHLRHLLETEAVNATERAEGQRLPGARLVTIAFAD
                     LVGFTRLGEAVPPEELEQLAHRLADLARDVAVPPVRFIKTIGDEVMLVSSDPVALLDA
                     VLDLVDATDGDDDFPRLRVGVATGMAVSRAGDWFGSPVNLASRVTGAARPGSILVSES
                     AREAIGDDDRFTWSFAGARHLKGIKSEVKLFRARRASTESD"
     gene            11910..12493
                     /locus_tag="VFC01_00660"
                     /pseudo
     CDS             11910..12493
                     /locus_tag="VFC01_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
BASE COUNT         2040 a         4135 c         4293 g         2025 t
ORIGIN      
        1 cgctcgctgg cagtcttgga gccgtcgcta gccgccggga cgccgatcgt cggactggag
       61 ccgagctgta ctgcggcttt gcggcacgat gctcccgcgc tgctgccgga caacccactt
      121 gccgccgcaa cggcgaccgc gacgcgcacc ttcgccgagt tcctcaacga gtcgggctgg
      181 caaccgccgc aggtcggggg agaggcgctg gtgcagacac actgccacca gcacgcggtc
      241 gtcgggttcg acgcggaccg ctcggtaatg aaagccgccg gcatcgacgc gaccgtcccc
      301 gactccggat gctgtgggct cgcaggaaat ttcggcttcg aacgcggaca ctacgaggtg
      361 tcgaaggccg ccggagaacg tgtcctgctg cctgccgtgc gcgacgcagc accgacgacc
      421 gccatcgtcg ctgacggctt cagctgccgc acccagatca cgcacggcac ctctcgccaa
      481 ccggtccacc tcgctcagct gctggccgcc gcgctgcgtc aggggcgctg actcagcaag
      541 cgcagagccg tcagcgcata ggcgcgggcc gccacaagca gttcttcgat cgccacggat
      601 tcgtcggcgc ggtgggcctg tgtcgtagcc gagcccgggc ccaggaccac gaccggcacg
      661 ccgagatcgc gggcgacgaa tccgccgtcg caggcggccg tccaccctcc gaggggctgt
      721 ccggggccgc ccgcgtcggc caacgcggcg tcggtcaccc gagccagttc ggagtcggcg
      781 ggtgtctgaa aggccggcat ttccatcggc atcaccagat cgactgtcag gccacgttcg
      841 gccagccgca gaccggtcac ccgctcgcga agctcgtcga gcacggcgtc ggtcgactcg
      901 cccggcagca gcctgcgatc ggcgacgacc acacattccg ccggcacgat cgaaccgccg
      961 gtgccaccct ggatctgccc gacactccag gtcgccggcc ccagcagcgg gtgggggtgc
     1021 gtcgccagct cggcatgcag ccgctcgatc tcagcaacca ccgcggccgc accatagatc
     1081 gcgttcgccc cgtcatcggg attgcctgcg tgacaagcct ttccgtgaac ctcgatgcgc
     1141 aggtacgaag caccgcgggc gccgatgatg gtctgcaggt cggttggctc cgcggtgacg
     1201 cagcccaaga acgggcgccg caccgactcg acaaaggcgc ggatcccttt gccggtctcc
     1261 tcctcgtcga ccagcgctgt cagctcgacg ggtccgctca gcgcgacgcc gcgcagcgcc
     1321 gccaacgccg aaagcgacgc ggcgaggccg cctttcatat cggacgcgcc gcggccgtag
     1381 atacggccgt catgcaccgc accgccgaac gggtcgacgg tccacccgtc gccaaccggg
     1441 acgacgtcag tgtggccaag cagcagcagc ccgggccctg tgccgccctc gagggtgatg
     1501 cgcacgttgt cgcggcccgg ctccaccgcc gtttcgacga cgtccagccc aagctccgcg
     1561 gcggccgtac tgagcacacg aaccgtcgcc gcctcgtcgc ccggcggatt ctgcccggcc
     1621 gcgcggacca gcgacgtggt cagctcgacc agcgagccct cgtcgatcct gtcgagcacc
     1681 gcctgctccg cggcggacac gcccatccgg tcaacccttc gccagcaggt cgaccgcgcg
     1741 tcgcaaccgg gccactccct cgtaaatccg gtcgggcggc gtcgaggcga agcacaggcg
     1801 cagcgcatgc gtgaaccggc ctgacggcga gaacgccgga cccggtatga acgcgacgcc
     1861 ctcggcgagc gcccgctcga acaattcggt tgtgtcgacg ccattttcga gcgtcaccca
     1921 gaggaagaac ccgccctcgg gatcggtcca atgcgcgatg tcgccgaaat gctcgcgcag
     1981 cgccgtatgc atggcctcat tgcgccgctt gtactccgcc cgttgcgtga ccaggtggtt
     2041 ctccagatgc ccgccggtca ggaatcccgt caggagccgt tgcgcgggaa ggttggtgca
     2101 cgtgtccatc gcctgcttgg cgttgatcag cagcggctgc aactcgggca cggtgtcgac
     2161 ccacccgacc cgcagacccg gcgcgacaat tttcgagaac gttcgcacgg agaacaccag
     2221 gggatcaccg gcggcgagct cgcgcagcgt cggcaacgga tcgcccgcga atcgcagcag
     2281 accgtacggg tcgtcgtcga tcaccatcga cccccaggcg cgggcgagat ccagcagtcg
     2341 gtgtctgcgc tccagcgaaa gcgttacgcc agacgggttc tggaacgtcg ggatcgtata
     2401 gatggccttc ggtgtgcggc ccgcggccgc gacgagcccc tccagcgcgt cgatgtccat
     2461 gccgtcgtcg tcgaccggga tctccagcag ggtggcgccg taggagagag cggtcgcgct
     2521 gccgttggtg taggtcgggg actcgaccgt gacgagatcc cctgggtcca caaagatctt
     2581 gcagaacagg tcgagcccct gcatgccgcc ggcggtgatc gtcaaccggt cggcggtggt
     2641 ctcgtcgctg gtgccgcgca gcgcgtgcag cagggccgcg tgcaacggcg ggtcgccctc
     2701 ggtggcggcg tagtcgtagg cgtgtggctc ggcgagttca accgccgcga tggcggcgag
     2761 gatctccgac ggcaccgcct cggccgccgg actacccatc gcgaaccgga cgacgtcgtg
     2821 agtctgcttg tgcaacaagg acgtactcga atcgatcacc gacccgacca acccggttgc
     2881 gcgtgcggcg tacgggaggg ttgaggacct cacctgtcac ctccaaatcg tgagttcatg
     2941 cggcaagtcg gtcaggcgtt ccaccccggt ggcagtgacc acaaccggct cggacagttc
     3001 gtagccccag tcgtccatcc agatcccgag gatgatgtga aacgccatgc ccgcggccaa
     3061 ctccgttgtc tcgccgtgtc gcaggctcac ggtgcgctca ccccagtcgg gcggatatcc
     3121 gatgccgatc gagtagccga tccgcgaatc cttgcgtaac ccgtgtgacc ggatcacccg
     3181 gtcgaaggcc tcgtgcactg cgctgccggt gacgccgggg cggatcgcct ccagcgtggc
     3241 gtgcatcgcc tcagcgacaa tcttcgcggt ctcggtcagc cgctgcgggg gatcaccgag
     3301 catcactgtc cgagcgagcg gcgcgtgata gcggccgaac gccccggcca gctcgatggt
     3361 ggtggcttcg ccgttgcgga agggccggtc ggtccaagtc aggtgcggcg ttccggctgc
     3421 atcgccggtc ggcagcagcg gccacagggc ggggtaatcg ccgccgtgct cgggggtgcc
     3481 ggacgcctgc gcggcgagga tctcggcgac cacgtcgcac tgccgtcggc ccggccgcag
     3541 cccgtcaagg gcggtccgca tcgcgcgttc ggcgatcgtg ccggcggtcc gcagctgctc
     3601 cacctcgtag ggcgatttga tcagccgcac ccaattaacc agctcagccg agtcaaccag
     3661 ccggttctcc ggcagaccgt tatcgagcgc gagatagccg cggggcgaga agaaatgcgc
     3721 gtcgccctcg acgccgacga gctcaccggc cggcaccagt tcgcgggact cggcggcgat
     3781 ccagtcgaac gggtgcacat ccggccggtg caccagctcc tccgggtagc cgtgaatctg
     3841 gtccgccggc aggttgcagg tgaacgtcgc gccattcgcg tcctgagccc gcgcgaacag
     3901 atgcagttcg ccctcggctg ggacgaggag gcattgcggt gtgtagaagg accaggcgtt
     3961 gtacccggtc aggtaaaaca ggttcgccgg gtcacaaacg acaagtgcgc caaatgcacg
     4021 ctgggccatg gcttctcgta cccggcgcag ccgctcggtg tactccgctg tgtcgggggt
     4081 catcgcgcca gcaactgctg cccggggccg acggccttgc ggccgacgac ggtcagcgcc
     4141 gaccacatcg tcacttggtt ggcggtcagc accggcttgc cgagctcggc ctccaacgtg
     4201 gcgatcatgt cgtaggtcgg caggttggtg cagctgatga aaacggcttc ggcgtcgggc
     4261 cggtcggtgt cacggacgag ttgggcggtg acgtcataag gcaccgtcca gatgtcggag
     4321 gtcaggccga gcccggcggc cgcgaccact tcgatgcccg cctcggcgag gtagctgctc
     4381 aggccggcgg tgaggtcggc ggtgtagggc gtggccgtgg cgatcctcgt gatgtcgagg
     4441 tgcctgagtg cggccagcaa cgcgccgctg gtggtgacgg cggccggggc gccggcctgc
     4501 ttcatcgcgg ccaccagagc cgtctcgccg gcaagaccac cgacgaagct ggccgacgtg
     4561 catgcgtagg cggtgaccag cggggagacg gctttcaact cgctaacgcc attggcgacc
     4621 accgacgggt cggagatgtg ggcgaccatc tcgatagtca cggcgagcgg aacgtagggc
     4681 gtccgggtca ggtgcaggct gatgtcgtcg ggcacccagc gccacagttc ccgatcgagg
     4741 gcaaagtcgt acggcaccac gacaccgatc ccggtctgtt ctagaggagg ggatacggcc
     4801 atggccccag tctgggggga cgcccccgcc aacgcaaccg attgttgaca atcggacagt
     4861 ttcgttccta gcctcgaacg tgtgccgacc cgaccggtag ttgcggtgct ctgtgagcgg
     4921 gcgactgaca ggccgcccgg gcttgacgca ctcgatgtcg acttccggta ttgcgccgcc
     4981 gacgggctgg ccgaggcggt ccgcggagca cgtggattgc tgctgtggga tttcttctcc
     5041 accgcggtcc gcgacgtctg ggccgatgcg ggcaccgtcg agtggatcca tgtcaccgct
     5101 gccggagtcg acaccctgct ctttgacgag ctgcgcgact ctgacgtcgt ggtgaccaac
     5161 gcgcacgggg tgttcgaccg gcctatcgcg gagtacgtgc ttggcgcggt gatcgcgtac
     5221 gccaaggaca gtcgccgcag cttcgaccta cagcgtcgac accagtggca gcaccgcgag
     5281 acacgcacgg tggccggcgc caccgccctc gtggtgggca caggcgggat cggccgggag
     5341 atcgcacggc ttcttcgcgc ggccgggatc gaggtcaggg gcgcgggccg caaggcacga
     5401 gacgacgacc ccgacttcgg caaagtcgtt gccagcgggg acctttcggc cgaggtcggg
     5461 tggtgtgatc acttgatcct ggccgcaccg ttgaccgacg acacccgcgg cctcgtcgac
     5521 gcggccgtgc tgagcgcgat gaagccggac gcgcatctgg tcaacatcgc ccgcggcccg
     5581 ctcgtcgacg aggccgcttt gctcgcggca ctgaccggcg gccggatcgg cggtgcgaca
     5641 ctcgatgtct tcaccgagga gccgctgccc gccgaccatc cactgtggga cgcgccgaat
     5701 gcgacgatca ctgcgcacat gtctggtgac gtcgtcggct ggcgtgacac tctggccgcc
     5761 cagtacgcgg agaacgtccg gcggtggctg gccggcgagc ccttgctcaa cgtggtggac
     5821 aagaagcttg gctacatccc gggagggtcg gcccgatgat tcctatggcg gtcgacctcg
     5881 tcgaggcgtt ccgacggaag gaattatcgc cggtcgaggc cactgcggcc gcgctggagg
     5941 cgatcgaccg ctacgacgat caggtgaatg ccttcgtgct cgtggacgcc gagggcgcgc
     6001 tggccgccgc gaaggagtcg gaggcccggt ggcacgcggg cgagccgctg ggtcccggcg
     6061 acggtgtccc cacgtcgatc aaggatgcgt tgtggacgcg cggctggccg acgctgcgcg
     6121 gcagcacgct gatcgacaag gcgggtccct gggaagagga tgcgccgagt gttgcgagac
     6181 ttcgcgagac gggcgcggtc atcatcggca agaccaccac ccccgagtac tcgtggaagg
     6241 gcgtaaccga ctcgttccgg cacggcatca cacgcaaccc ctgggatccc gggacgacag
     6301 cgggcgggtc cagcggcggc agcgccgcag ctgttggtct cggcatgggg ccgtggtcgg
     6361 tcggcacaga cggcggtggc tcggtgcgga tccccgcggg cttcaccggc actgtcgcgc
     6421 tgaagccgac ctacgggctg gtcgcgcact accccgcgag cgcgttcggc agtctctcgc
     6481 acgtcggacc aatgacgagg tcggtgcggg actgcgcggc gctactggac gtgatcagtg
     6541 gcttcgacgc ccgtgactgc acggccatgc cgacgccgac caagtcgttc ctggacgggc
     6601 tcgacaacgg cgtcgcgggc ctgcggatcg ccttctcgcc caacttcggg ttcgtgcgca
     6661 acgatcccga ggtggacatt gcggttcgag ccgccgtcga cgtaatcgcc tctgccggag
     6721 cggaagtgga cgaagtcgat ccgggattcg cagatcctgt cgaggcgttt cacgtgctct
     6781 ggttctccgg agaggcccac gttctgcggg cgtatggcga tgcggtcgac gaccgggtcg
     6841 accccggact ccggcggacc gccgcgatcg gcgcggccta ctccgcggcc gactacctcg
     6901 acgcgactgc cgtgcggatg gaactcggcg tgttgatggg caggtttcac cagacctatg
     6961 acgtgctcgt caccccgacg cttccgctgc cggcgttccc cgccgggcag gatgtgccgg
     7021 acggttggag ttcgccggac tggacgagtt ggacgccgta cacctatccg ttcaacctga
     7081 cgcagcagcc cgccatgagt gtcccgtgcg ggttcacctc ggctggactg ccgatcggcc
     7141 tgcaggtcgt cggtccgcgg cacgccgatg cattggttct tcgggtcggc caggcgtacc
     7201 agtccgccac cgactggcac ttgagaaccc cagctcttct gactgaggag gttcgatgag
     7261 cagatacatc accgtgtcgt tggacaagcg cagcgtgagc tgtgtcgccg tgttgctcga
     7321 caatgaggcg ccgcggacat gcgcggcggt ctgggatgcc ttgccgctgt cggcgcccgt
     7381 attccacggc aagtacgcgc gcaacgagat ctacaccctg ctaccgccat tcgcacccag
     7441 tgacccgggt aaggagaaca ccacgatcac cccgatcccg ggcgacctgt gctggttctc
     7501 gttcgattcc gaccagcttg gcaacccggc atacgggtac gagaacacca cgggaacggg
     7561 gacgacgggc gcgatcgtcg acctcgcgct gttctacggg cggaacaacc tgctgatcaa
     7621 cggtgaccaa ggatgggtgc cgggcaacgt cttcggcgcc gtcgtcgagg gactcgacga
     7681 gatggccgag gcctgccagg acctctggat gggtggtgtc cgcggagaga cactcagctt
     7741 cagccgccgc gactgacggg gcgtttcgta gcctggtgtc gtgggcgtaa ggaaagccac
     7801 gacaccgtcc gcgccggtcc gcgggctggt cggccgcgcc ggcaccacgt cgctgtttga
     7861 tctgcatcgg tggcagccca cgggcgttgc agcccagttc gtcgagcact actggtcggt
     7921 cagctgggac ctacgcgacc cgtacgagag cacggtcatc accttcccgg ccgtgcacct
     7981 gaccaacgag tggggtaccg acggcagccg gcacggccac accttgccag cggtgctgct
     8041 gcacggtgtc gtcgagcgcg tgtttcgcac cacgatccgc ggcacaggca cggtcgtcgg
     8101 cgcgcgcttt caccctggtg gtttcacggc gcgctatggc cgtgatgccg ggtcgttgaa
     8161 gggcagggtc gagcgggcgg aggaactgct gcccgatgcg ccgcgcgacg tcgaaaactg
     8221 cttggccgca ttggaatccg cgatcggatc cgacgcgtcg ctcgatgata cgtatgtgcg
     8281 gctgcgcaag ctggtcgaac tgatccgcga cgacgatcgg ctgcaccggg tggagcaggt
     8341 gatggagcat tcgccgtgga gcgcgcgcac tacgcagcgg gtgttccgcc gctacgtcgg
     8401 cgttccggtc aaatgggtgc tgtgccgtta ccggctgcag caggcggccc tggagatcga
     8461 gagccggccc ggtgtcgacc acgctgacct cgccgcccgc ctgggttggt acgaccaggc
     8521 ccatttcatc aacgacttcc gcacaatgct tggcagtact cctggcgaat acgccgccag
     8581 ttacgggagt taggagattg cgtggatagt cgtgagctcg cgttcgccgg tgccgcgcag
     8641 caggcgcgga tgctcgccga tggcgccatc accgcgccgg agctgttgga gctgtacctg
     8701 gatcgcatcg cgcgactgga tccccaactg cgctcgtacc gcgtcgttct cgccgattcc
     8761 gcacgccagg aagccggcgt tgcccaggat cggctcaatg cgggggagcg gctgcctctg
     8821 ctcggcgtgc cgatcgcgat caaggacgac gtcgatgtcg ccggcggggt gacgacgtac
     8881 ggcagcgccg cgcacgggcc ggcgtgcacg caggacgcgg aggtggtgcg ccgactgcgt
     8941 gaagcgggtg ccgtcatcgt cgggaagacc aatgtgcccg aactgatggt gtggtcgttc
     9001 accgagacac tggcgtacgg ggcgacacgc aatccctgga acaccgacta cacgccaggc
     9061 ggcagcagcg gcggcagtgg cgccgccgtg gcagcaggct tggctgctat ggcgctgggt
     9121 tcggacggca tgggttcgat ccggattccg tcgacgtggt gcgggctgtt cggcatcaag
     9181 ccgcagcggg atcgggtgcc gctggcgccg cacgacgacg cgtggaacgg cctgagtgtc
     9241 aatggcccca tcgcgaggac ggtggaggac gccgcgctgt tcttggacgt gacagctccc
     9301 ggcaatgagt tcgtggccgc gaccaagcgg ccgccgggtc agttgcggat tgcgttgagc
     9361 gccaaggcgc ctccgggttt ggtcgtgcga gtcgggcggg cccagcgagc ggcgtttgac
     9421 gaagcaggcg cgctgctgcg agagctcggc cacgaggtgg tcgagcgtga cccggactat
     9481 ccgccggccg cggtctatgg acaggcgttg ccgcggtatt tccgaggggt ttacgacgat
     9541 gtgaaggcgc tgccgcatcc ggagcgactc gacgccagga cgcgtgcgtt cgcgaggatc
     9601 ggcgggctga tctccgaccg tcggatgcgt gcgatcaggg gagcggaaag catagcggcc
     9661 gaacggattc agtcgatctt tgatgatgtc gacgtcgtca tcacgccagg taccgcaatg
     9721 ggcccttcgc gcatcggggc ataccagcgg cgcggagcga tacccacgtt ggcgttggtg
     9781 gcggcacgcg tgccgttcca ggcgatgttc aatgtgacgg gccagccggc agcggtggtg
     9841 ccgtgggatt ctgatggcaa cggcgtgccg acgtcaatcc agctggtggg caggccgttc
     9901 gacgaggcga cgttgctctc gctgggcgcg cagatcgagc aggcgaggcc gtgggcgcat
     9961 cgccgcccgc cggtgtcatg actcgtcgat gatccgccac gcggcgagtt tgtcttcgta
    10021 gcgcatggtc tgcgcagggc tggtcgacgg caaccgtcgg tattgaaaat cgggcatgac
    10081 ccggaccaat cggttgaagt tcttctctgc tgcggccccg ttgaagaaca cccgggtgat
    10141 actcggatgc cgattgaaaa atcgttggaa gtcgttggcc accatgctgt ctggctctac
    10201 ggcggaatcc aggctgccga tgcgacggca tgaccgcagc acatcccaca ccgcgatgcc
    10261 atgtgccgtc agcgtcgcgg tacgcacgtc atacagtgct gaggcgtcga acccgtagat
    10321 ctcgccggtg atcctccaga acgcgttccg cggattggcg taatactgct gcgaagctag
    10381 cgacatcacg ctcggcatat tgccgagaat cagcgtgtgg gccccgtcgg cgatgatcgg
    10441 cggcaaccca tgaagcagct cggaggtcac gtatccgaat atcgcacctt gttcgcggta
    10501 tacgttgtgg ccgtggccga agagttcgat ctcgaagcgt ccgggctgct cgacggcctc
    10561 gagggcaagg cacgcgacga gcgcgccgaa ctgatcccgt ggctgctcga gcgcggcatc
    10621 accgtcgagc agattcgggg tgcctatgcg cccgcgttgc tgggcgcgcg caagatcctc
    10681 ggcgacgacg gcacctacgt ctcggcgcgc gaggccagcg agaagaccgg catcgacctc
    10741 gacctgcttc aacgcatcca gcgggccatg gggctgccgc gggtcgacga tcccgacgcg
    10801 gccgtacacc tgcgtgccga cgccgaagcc gcggcattcg cgcagaagtt cctcgaactc
    10861 ggcatcgacc ccgatcaact tgttcaggtg agccggatac tcaacgaagg gttgtcacgt
    10921 gccgccgaag tgatgcgaaa cgccgcgttg gtttcggtcc tcaatccctc cgccacggag
    10981 ttggacatcg ccaagaactc cgaagcgttc ttgagccagg ccgccccgat gctcgggccg
    11041 atgatccagg aaatcctccg attgcatttg cgccacctgt tggaaacgga agccgtcaac
    11101 gcgaccgaac gggccgaggg ccagcgcctt cccggcgccc ggctggtcac catcgccttc
    11161 gccgatctcg tcggcttcac gcggctcggc gaagcagtgc cgcccgaaga gctcgagcaa
    11221 ttggcgcacc ggttggccga cctcgcccgc gacgtggccg tcccgcccgt gcggttcatc
    11281 aagaccatcg gcgacgaagt gatgttggtc agttcggatc cagtggcgct tcttgacgcg
    11341 gtgttggatc tggtcgacgc caccgacggc gacgacgact tcccccgcct gcgcgtcgga
    11401 gtcgccaccg gcatggcggt gagccgggcc ggcgactggt tcggcagtcc ggtgaacctc
    11461 gccagccgtg tcacgggggc agcgcggccg ggttcgattt tggtctcgga atcggcacgc
    11521 gaggcaatcg gggatgacga ccggttcacg tggtcctttg ccggcgcgcg tcacctgaaa
    11581 ggcatcaagt cagaggtcaa gctgttccgc gcgcgtcgag cttcaaccga aagcgactga
    11641 tctccgagga agttatcccc agacgcgaat tcatccacag gctgggcgtg acgacggcgc
    11701 ctggtcgcat gatcgaacta gtgttcgaga catgagtacg ggtgcggtat cggatcgcga
    11761 ggacatcctc gcggtcgtcg cgcgctggga acaggcccaa gccgagttgg caggcctgtc
    11821 ctttagcgcg ctgacctgac ccgaagctag ccgtccaagg tcggctggag aagggaaatc
    11881 gccgccaacc cgcggtcgat cacaaactga ttcaccagct caccttgcaa agcacgccga
    11941 ccgaattggg cgccaacacc tggcccaagg tgctctccga agcgctgctt atctccccgg
    12001 gcgaggccaa acgacgcctc aagcaagctg aattgttggg gccgcgcacc gcgttgaccg
    12061 gtgaagcatt gccgccgcag ctgcccaatg tggccgccgc gcaggaacgc ggcgaggtcg
    12121 gtccgaacac ctgcggatta tcgagaagtt cttcgacgag ttgccgagcc gcatcgactc
    12181 ccagacccgc gagttggccg aggccgatct ggcccgtatc gcgaccgggt tggggccgac
    12241 gcaatttcgc gcggccgccg accggttggc gctgctactc aaccaggacg gcgaggaacc
    12301 cgatgacgcc gagcgcgccc gccgccgcta tctgatgatc gacaagcagg acatcgatgg
    12361 gatgagccgc attcatggcc tgctcgaccc ggaagcccgt gccaccatcg atgcggtgtt
    12421 cgccaagctg gccgcaccgg ggatgtgcaa acccgacgac gaatcaccct gcgtggaccg
    12481 cgaacccagc gac
//
LOCUS       DASRJA010000015         8117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2528709,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000015 DASRJA010000000
VERSION     DASRJA010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2528709"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFC01_00665"
     CDS             <1..358
                     /locus_tag="VFC01_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN78189.1"
                     /translation="EDGYCNIVGRIKDMVIRGGENIYPREVEEFLYTHPDIEDAQVIG
                     VPDEKYGEEICAWIQMKAGRPALDDAAVKAFATGKLAHYKIPRYVHVVDDFPMTVTGK
                     VRKVEMREETVKLLGL"
     gene            457..1308
                     /locus_tag="VFC01_00670"
     CDS             457..1308
                     /locus_tag="VFC01_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78190.1"
                     /translation="MGEIFIGSEAVANSDVTRHELQRWYRPLYPNVHLARGNDVSLRD
                     RTVGGWLWSKRNGVVTGVAASALHGAEWVDDDVAIELLWNCTRPPDGIVVRNERVSDD
                     ELTWVAGLPVTTPARTAFDLGRYLKRGQAIARLDALMRAAPFSVEDVMLLTKRYKGAR
                     GVAKLKAVLPLVDGGAASPQETRLRLLFIDAGLPRPTTQIPVYDECGRFVRFLDMGWE
                     DFMVGAEYDGDQHRTSRPQYVKDQRVWPKLRQLGWHVVRAIKEDRDDDLVRQAWDAMA
                     SRGWRPN"
     gene            complement(1379..2095)
                     /locus_tag="VFC01_00675"
     CDS             complement(1379..2095)
                     /locus_tag="VFC01_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZN78191.1"
                     /translation="MSPADGTLSGRQPKAVQSALRVLEEVARAGVGVTAKEIAENLSM
                     PSATAYRLLNLLVADGYIVRLPDLSGFSLGNRMGVLIDAAVAPTVCTAAREVLAELRL
                     SVRFGVHLYYFTNTAMRAADLDSEFPPPVDEAELNRNLHRSAVGRLLLAEKQDLAGLV
                     PVSQELLARLDDVRQQGFATQTGELREDCACVAVPVRAPSGGLVAALGMSGRADQDLL
                     LGKQVPALTECAKRLSPLLA"
     gene            complement(2092..3549)
                     /locus_tag="VFC01_00680"
     CDS             complement(2092..3549)
                     /locus_tag="VFC01_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN78192.1"
                     /translation="MGYVRPIRSSSPVSGLRRGQLSFAQVLGQSVSAVAPSAVMVTLP
                     ALVIPAAGGWTLGVFVVTALLMAAVGYCVGQFATRMVAVSGLYSYTVKGLGPGAGIAA
                     GWSLVIGYAAAAMASSVGAANYLAALLSRLGVPSGAVTITVLALVVGAVALTLMLRGI
                     RLSARISLTVEVFAIVVAAAVLVIAFGGSIAGGRAPPDVPVEQPHSALGFALLLAISS
                     FVGFESAGTVAREARRPFVTVPRAIRWTPLAVGVLYVFAAAMQSAQTIRAGIGSVPIV
                     LTLPASAGAGSTALSIAMELGITASWFACIIGSTTALSRTLFAMGREGVVPEAVGSTN
                     RRFHTPHVALLLAMPVIVVVPLAYLWVTGSSREVLIGLLAVSAHGYVGAYLLVCLATP
                     VFLRRIGELTRLPLVIGVTTAVLMIAIILWAALTLESPVWIATAVYAGLLAAGFALYA
                     FRLRRVPGLTERVGVFDETVEGDLLADYNPWMVRR"
     gene            3772..5274
                     /locus_tag="VFC01_00685"
     CDS             3772..5274
                     /locus_tag="VFC01_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN78193.1"
                     /translation="MTIAAEATRLDPRVVEFVSTPKQCYVDGQWVDALSGRRFETVDP
                     ATEAVITTVPHSGAEDVEAAVRAARRAFDDGPWPSLTPAERQRLIWRIGEGILARADQ
                     FAQLETLDNGKSVAVARAVDVTWAAEIFLYYAGWATKIEGRTIPVSVPWAPGARFHAF
                     TLREPVGVCAQIIPWNFPLVMAAFKVAPALACGNTVILKPAEQTPLTALLLAEVIAEA
                     GVPDGVVNVLTGFGDIGAALSAHPDVDKVAFTGSTEVGRKIVNAASGNLKKVSLELGG
                     KSPQVVFADADLDAAIPGAASGFLFNHGQTCTAGTRLLVEDRIFDEFTEGVADLARAQ
                     KIGPGLDPTNDIGPLVSREQLTKVTGYLDAGICDGARALAGGGRHGDTGFYVQPSLLV
                     DVRPDFSVYREEIFGPVAVAIPFNREQGVRAAANDTPYGLAASVWTRDVSTAHEVAAQ
                     IKAGTVWINCHNAFDTALPFGGYKQSGWGRELGEGAIAEYTQTKAVNMAL"
     gene            5387..6838
                     /locus_tag="VFC01_00690"
     CDS             5387..6838
                     /locus_tag="VFC01_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN78194.1"
                     /translation="MTTDALASGPQVRDREPHRLAGRLGPAAIVFMVVAAAAPLTVVA
                     GTFPIGIAAGNGAAYPASYLVCTVILILFAVGFTAMARHVPGAGAFYTYVGHGLGRHM
                     GLGAAFLALLSYTAVQGGVYGYVGAAINDLVTSHGGPSVPWYVYGLAMMAVVATLGYR
                     HIELSGKVLGVLLICEVGIVMVINLAVIGHGGDGGMSTAAFDPGKFFTGAPGIALIFA
                     LAGYIGFEATAVFRDEARDPARTIPRATYLALLLIGGFYALSSWALVSAWGDEGAVAM
                     ATHNPEGMLTETATRYVGTVAGDLVQIFLITSLFAALLSFHNVLARYVFSLGNTAALP
                     SSCGRSHARHASPHIASAVQTVSALVLVIASALAGLDPVTQVFAWFVGISSVGIIALM
                     TLTSVAVVVYFRRSRVDTRTWHTLVAPGLGLAGLAVLLVMTAINLPLLVGGSSTTAAV
                     IGVLLVGTFAGGAVLATLRPRAGRPSTEKEYSR"
     gene            6835..>8117
                     /locus_tag="VFC01_00695"
     CDS             6835..>8117
                     /locus_tag="VFC01_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyramine oxidase"
                     /protein_id="HZN78195.1"
                     /translation="MSTATIETVDQTASAAPDHPLTPLTADEIRAVRRIVDAHRLLGE
                     SVRFVYTALDEPHKETVLAFSPGDPIERRARVLLLDRATGLGTDVVVSITGDRVVTQQ
                     SIDAGSEGHVPILDQEFEDIEAFLLESSEWLDAMHKRNLNPTDVRAVPLSAGVFGHED
                     EVGRRVVRVLAFYQYDKADLPWAHPIDGVVAYVDLTARRVTKVIDELDLAVPAERGEW
                     DAEPHAVPARTDLKPIEITQPEGPSFSVDGNQITWADWTFRFGFDIREGLTLHQIAIG
                     GRPVVYRASIAEMVVPYADPSPVRYWQNYFDQGEYLFGRYTNSLELGCDCLGEIKYFD
                     VTIADEQGEPKVLKNAICLHEEDYGVLWKHTDMFNGMAETRRSRRLVISFFLTIGNYD
                     YGFYWYLYLDGTIQLEAKATGIVFTSAYRGPDGFAT"
BASE COUNT         1461 a         2760 c         2577 g         1319 t
ORIGIN      
        1 agaagacggc tactgcaaca tcgttggccg catcaaggac atggtgatcc gcggcgggga
       61 gaacatctac ccccgcgagg ttgaggagtt cctctacacc catcccgaca tcgaggatgc
      121 ccaggtgatc ggcgtccccg acgagaagta cggcgaggag atctgcgcgt ggatccagat
      181 gaaggcgggc aggcccgcgc tggacgacgc cgctgtgaag gcattcgcaa ccggcaagct
      241 ggcgcactac aagatcccgc gctacgtaca cgtcgtcgac gacttcccga tgacggtcac
      301 cggtaaggtg cgcaaggtcg agatgcgcga ggagacggtg aagctgctcg gcctctaatt
      361 tccgccgaaa cggtattcca gcaggaaaag tgcgagtaga gacctgccgg aatacagttt
      421 cgcgggcttg tcggtggtcg cgaccaggat gcggccatgg gggagatttt tatcggcagt
      481 gaggctgtcg ccaacagcga cgtgacgcga catgagcttc agcggtggta tcgaccgctc
      541 tatccaaacg ttcatctggc gcgaggtaac gatgtgtcgc ttcgagatag gaccgtcggc
      601 gggtggctgt ggtcgaagcg caacggcgtt gtcactggcg tggccgcgtc ggccttgcat
      661 ggtgcggaat gggtggacga cgacgtcgcg attgaactgc tctggaattg cactcggccg
      721 cccgacggca tcgtcgtccg caatgagcgc gtcagcgacg atgaactgac atgggtggcg
      781 gggcttccgg tgacgacgcc cgcgcgtacg gcgttcgatt tgggcagata tctcaagcgc
      841 ggccaggcga tcgcgcgcct cgacgcccta atgcgtgcgg cgccgttctc cgtcgaggac
      901 gtcatgttgc tcaccaagcg atacaagggc gcgcgaggcg tcgcgaaact gaaggcggtg
      961 ctgccgctcg tcgacggtgg cgctgcctcg ccgcaggaga cgcgcttgcg gttgctgttc
     1021 atcgacgctg gtttgcccag acccacgacg cagatacccg tctacgacga gtgcggtcgg
     1081 tttgtgcggt tcctcgacat gggttgggag gacttcatgg tcggtgctga gtacgacggc
     1141 gaccaacacc ggacgagccg accgcagtac gtcaaggacc agcgggtgtg gccgaagctc
     1201 cgacaactgg gttggcatgt cgtacgcgct atcaaggagg accgtgacga cgacctcgtc
     1261 aggcaggcct gggatgcgat ggcgtcgcgg ggttggcgtc ccaattgaga atgccgaaac
     1321 ggtattccag cagggaaagt gcgagtagtg gcctgccgga atacagtttc ggcgaaaact
     1381 aggccagcag cgggcttagc cgctttgcgc actcggtcag tgccggtact tgcttgccca
     1441 gcaacagatc ctggtcggcg cgacccgaca tacccaacgc agcgaccagc ccgccggacg
     1501 gcgccctgac cggcaccgcg acgcacgcac agtcctcgcg aagctcgccg gtctgcgttg
     1561 cgaaaccctg ctgccgcacg tcgtcgagcc gcgccagcag ctcctggctg accggaacca
     1621 acccggcgag atcctgcttc tcggccagca gcagccggcc aacggcagag cgatgcagat
     1681 tcctgttcag ctccgcctcg tcgactggcg gcggaaactc gctgtcgaga tccgcggccc
     1741 gcatcgcggt gttggtgaag tagtacaggt gcacgccgaa gcgcaccgac aggcgcagtt
     1801 cggcgagcac ctcgcgggcc gccgtgcata ccgtcggcgc gaccgccgca tcgatcagca
     1861 cgcccatccg atttccgagc gagaaaccgg acaggtccgg caggcggacg atatacccat
     1921 cagccacaag cagattcaat agccgatacg cggtcgccga cggcatcgac aggttctccg
     1981 cgatctcctt tgccgtcaca ccgacgcctg cccgcgccac ctcctccagc actcgcagcg
     2041 cgctctgcac cgccttcggc tgccgccccg acagcgtgcc gtccgccggg ctcatcgccg
     2101 caccatccac gggttgtagt ccgccaacag atcaccctcg acggtctcgt caaacacccc
     2161 gactcgctcg gtcaatccgg gcacccgccg cagccgaaac gcgtacagcg cgaagcccgc
     2221 cgcgagtagt ccggcataca ccgcggtcgc gatccacacc ggcgactcca atgtcaatgc
     2281 ggcccaaagg attatcgcga tcatcagtac cgctgtcgtc acaccgatca ccagaggcag
     2341 cctggtcagc tcgccgatac gacgcaggaa caccggtgtt gccaggcaca ccaacaggta
     2401 cgcgccgaca tatccatggg ccgagaccgc gagcaggccg atcagcactt cccggctgga
     2461 accggtcacc cacaggtagg ccagcggcac caccacgatg actggcatcg cgagcaggag
     2521 cgcgacatgc ggtgtgtgaa aacggcgatt ggtgctgccg acggcctcgg gcacgacacc
     2581 ctcgcgtccc atggcgaaca gggtgcgcga cagggcggtc gtggaaccga tgatgcaggc
     2641 gaaccatgac gccgtgatgc cgagctccat tgcgatggac aatgccgttg agcctgcgcc
     2701 tgcgctggcg ggcagcgtca gcacgatcgg gacggaaccg attcctgccc tgatggtttg
     2761 ggccgactgc atggccgcgg cgaagacgta gagcacgccg accgctagcg gggtccaccg
     2821 gatcgcgcgc ggcacagtga cgaacggcct acgggcctcg cgtgcgaccg tccccgcact
     2881 ctcgaagccg acgaaggagc tgatcgccag caacagcgcg aagccgagcg ccgagtgagg
     2941 ctgctcgacg ggcacgtcgg gcggcgcgcg gccacccgcg atggagccgc cgaatgcgat
     3001 gaccaacacc gccgccgcga ccacgatcgc gaatacctct acggtcaacg agattcgggc
     3061 ggatagccgg attcccctga gcatcagcgt cagcgcgacc gcgccgacga ccagagcaag
     3121 cacggtgatc gtcaccgcac cactcggcac accgaggcgg gacagcagtg ccgcaaggta
     3181 attcgccgct ccgacagaac tggccatcgc cgccgcggcg tatccgatca ccagcgacca
     3241 cccggcggcg atcccggccc ccggaccaag gcccttgacc gtgtaactgt agagcccgct
     3301 gactgcgacc atccgcgtgg cgaactggcc gacgcaatac ccgaccgcgg ccatcagtag
     3361 cgccgtgacg acgaagacac cgagagtcca gccgccggcc gccgggatca ccaatgcagg
     3421 cagcgtgacc atgaccgccg acggcgcgac cgcagacacc gactgaccca agacctgcgc
     3481 gaacgacaac tgtcctcgcc gcaagccgga gaccggtgag ctgcttctga tcggccgcac
     3541 gtagcccatc ctcccgcgtc gaccgccttc tgcacgttaa ctttgcggat cgtcaaatga
     3601 gaatcacgat cacaccccga cgcctaaccg ctggataatg cgcgccaaca gggacaaacc
     3661 gccgtctgga atgagaatcg gaccgattat ggaatgagaa tgtgaattcg tcgaattccc
     3721 attcgaaatt agtagtgact tcactcacat cgcggcgtta cttttgcccg catgactatt
     3781 gccgccgaag caacgaggct cgatccgcgc gtcgtcgaat tcgtgtcgac gcccaagcag
     3841 tgctacgtcg acgggcagtg ggtcgacgcg ctctcgggtc gccgcttcga gacggtcgac
     3901 cccgccaccg aagcggtgat caccacggtg ccgcacagcg gagccgagga cgtcgaggcg
     3961 gctgtgcggg cagcgcggcg cgcattcgac gacgggccat ggccgtcgct caccccagcc
     4021 gagcggcagc gcctgatctg gcgcatcggc gagggcatcc tcgcccgcgc cgaccagttc
     4081 gcgcagctgg aaaccctcga caacggaaag tcggtggcgg tcgcacgcgc cgtcgacgtc
     4141 acgtgggccg ccgagatctt cctgtactac gcgggctggg ccaccaagat cgagggccgc
     4201 acaatcccgg tgtcggtgcc gtgggcaccg ggcgcccggt tccacgcctt caccctgcgc
     4261 gagccggtcg gcgtatgcgc gcagatcatc ccctggaact tcccgctggt gatggcggcc
     4321 ttcaaggtcg cgcccgcact cgcatgcggc aacaccgtga tcctcaagcc ggccgaacag
     4381 acgccgctca ccgcgctgct gctggccgag gtcatcgccg aggccggcgt cccggacgga
     4441 gttgtcaacg tgctcaccgg attcggcgac atcggcgcgg cgctgtcggc gcatcccgat
     4501 gtcgacaagg tcgcgttcac cggttcgacc gaggtcggcc gcaagatcgt caacgccgca
     4561 tctggcaacc tcaagaaggt ctcactggaa ctcggcggca agagcccgca ggtcgtcttt
     4621 gccgacgccg acctggatgc ggcgatcccc ggcgcggcca gcggcttcct gttcaaccac
     4681 ggccagacct gcacggcggg cacccggctt cttgtcgagg accgtatctt cgacgagttc
     4741 accgaggggg tggccgacct cgcgcgggcg cagaagatcg gcccgggcct ggacccgacc
     4801 aacgacatcg gcccgctggt ctcgcgcgag cagctgacaa aggtcaccgg ctacctcgac
     4861 gcaggcatct gcgacggcgc ccgcgccttg gccggcggcg gccgccacgg cgacaccggg
     4921 ttctacgtcc agccgagcct gctcgtcgac gtgcgacccg acttcagcgt gtaccgcgag
     4981 gagattttcg ggccggtcgc cgtcgcgatc cctttcaacc gcgagcaagg tgtgcgcgcc
     5041 gctgccaacg acaccccgta cgggctggcg gccagcgtgt ggacccgcga cgtgtcgacg
     5101 gcccacgagg tcgccgctca gatcaaggcg ggcacggtgt ggatcaattg ccataacgca
     5161 ttcgataccg cccttccgtt cggtggctac aaacaatccg gctggggccg cgaactcggc
     5221 gagggcgcca tcgccgaata cacccaaacg aaggccgtca atatggccct ctgacagaaa
     5281 tccccgtcaa atgagaattc gctgcagagc cattctcatt tcagcttcgg caagcttcac
     5341 gacgccgcaa cccaaaaccc attccccaga cgaaagcctg attgccatga caaccgatgc
     5401 cctcgcgtcc ggaccccagg tccgcgaccg cgaaccccac cgcctcgcag gccggctcgg
     5461 cccggccgcg atcgtcttca tggtcgtggc ggccgccgcg ccactcaccg tcgtcgcagg
     5521 gacattcccg atcgggatcg ccgccggcaa tggcgccgcc tacccggcgt cgtatctggt
     5581 gtgcaccgta atcctgatcc tcttcgcggt cggtttcacc gcgatggcca ggcatgtgcc
     5641 gggcgccggc gccttttaca cctacgtcgg tcacgggctg ggcagacaca tggggcttgg
     5701 cgccgccttc ctcgccctgc tgtcgtatac cgcggtgcag ggcggggtct acggctatgt
     5761 gggcgcggcg atcaacgacc tcgtcacatc gcacggtggc ccgtcggtcc cgtggtacgt
     5821 ctacgggctg gcgatgatgg ccgtggttgc gacccttggc taccggcaca tcgaactatc
     5881 cggcaaggtg ctcggcgttc tgctgatctg cgaggtcggc atcgtgatgg tgatcaacct
     5941 cgcggtcatc ggccacggcg gagacggtgg aatgtccacg gccgcttttg atcccggcaa
     6001 gttcttcacc ggtgcgccgg gtatcgcgct gatcttcgcg ctggccggct acatcggttt
     6061 cgaggccacc gccgtcttcc gcgacgaggc acgcgaccct gcgcggacca ttccacgcgc
     6121 gacctatctc gcccttctgc tcatcggcgg cttctacgcg ctatcgagtt gggcgctggt
     6181 cagtgcgtgg ggcgatgagg gcgcggtggc gatggccacc cacaaccccg agggcatgtt
     6241 gacagagacc gccacccgct acgtcggcac cgtcgctggc gacctcgtcc agatcttctt
     6301 gatcacgagc ctcttcgccg ccttgctgtc cttccacaat gtgcttgcgc gctatgtctt
     6361 ttcgctcggc aacaccgccg ccctgccgtc gagctgcggc cgcagccacg ccaggcacgc
     6421 ctcgccgcac atcgcctcgg cggtgcagac cgtctcggcg ctagtcctcg tcatcgcgtc
     6481 ggcgctggcc ggtctcgatc cagtcactca ggtgttcgcc tggttcgtgg ggatctcctc
     6541 ggtcggcatc atcgcgctga tgacactgac gtccgtcgcg gtcgtcgtgt acttccgccg
     6601 cagcagggtc gacaccagaa cctggcacac cctcgtcgcc ccgggcctcg gattggccgg
     6661 cctcgcagtc ctgctcgtga tgaccgccat caacctgccg ctgctcgtcg gcggttcgag
     6721 cacaacggcc gccgtgatcg gcgtgctgct ggtcggcacc ttcgcaggcg gcgccgtgct
     6781 cgcgacgctg cgccctcgcg ccggccgccc gtcaacagaa aaggagtact cccgatgagc
     6841 accgccacca tcgagaccgt cgaccaaacc gcctctgcgg caccggatca tccgttgacg
     6901 ccgttgaccg ccgacgagat ccgcgctgtg cgccgcattg tcgacgcgca caggctactc
     6961 ggtgagagcg ttcggttcgt ctacaccgcg ctcgacgagc cacacaagga aaccgtgctg
     7021 gccttctcgc cgggcgatcc gatcgagcgc cgtgcccgag tcctgctgct ggacagggca
     7081 accggtctcg gcaccgacgt cgtggtgtcg atcaccggtg accgcgttgt gacacagcag
     7141 tcgatcgacg ctggaagtga gggccacgtt ccgatcctcg accaggagtt cgaggacatc
     7201 gaggcgtttc tgctcgagag ctccgaatgg ctggacgcga tgcacaagcg caacctcaac
     7261 ccgactgacg tgcgcgccgt tccgctgtcg gcaggcgtgt tcggacacga ggatgaggtg
     7321 ggccggcgcg tggtccgtgt gcttgccttc taccagtacg acaaagccga cctgccgtgg
     7381 gcgcacccga tcgacggggt ggtggcctac gtcgacctca ctgcgcgccg ggtgaccaag
     7441 gtgatcgacg aactcgacct cgcggtgcct gccgaacgag gggagtggga cgccgaaccg
     7501 cacgccgtac ctgctcgcac cgacctcaag ccgatcgaga tcacccaacc ggaaggtccg
     7561 agcttctccg tcgacggtaa ccagatcacc tgggcggatt ggacattcag gttcggcttc
     7621 gacatccgcg aaggtctgac gctgcatcag atcgcgatcg gcgggcggcc ggtggtctac
     7681 cgcgcgtcga tcgccgagat ggtggtgccg tacgccgacc cgtcaccggt gcgctactgg
     7741 cagaactact tcgatcaggg cgagtacctg ttcggccgct acaccaactc gcttgagttg
     7801 ggctgcgact gcctaggcga gatcaagtat ttcgacgtga cgatcgccga cgagcagggc
     7861 gagccgaagg tgctgaagaa tgcgatctgc ctgcacgagg aggactacgg cgtgttgtgg
     7921 aagcacaccg acatgttcaa cggcatggcc gagacccggc gctcccgacg actcgtcatc
     7981 tcgttcttct tgaccatcgg caactacgac tacgggttct actggtacct gtacctcgac
     8041 ggcacgatcc agctcgaggc gaaggcgacc ggaatcgtgt tcacgtcggc gtaccgcgga
     8101 cccgacgggt tcgccac
//
LOCUS       DASRJA010000016         5056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3370837,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000016 DASRJA010000000
VERSION     DASRJA010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3370837"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..974
                     /locus_tag="VFC01_00700"
     CDS             33..974
                     /locus_tag="VFC01_00700"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN78196.1"
                     /translation="MSASTLKIGWIGAGRMGAQLVTRLLDAGYDVTVYNRTASKAQHL
                     ADRGAGIVSRPVDLADRDLVFSMVSSSKDLEQVMLGEGGLLTGAASPRVIADVSTVSA
                     DASALIREAANSRGSDFLATPVSGNPKVIAAGKLTVAVSGPREVFESIEPVLNVFGRS
                     VTYVGEGEVARLVKIAHNLMLGVVTQCLAEITVLVEKGGVSRADFLAFLNDSVMGSVF
                     TQYKSPAFVNLDFTPTFTMQLLQKDFDLGLEAANQLKSPMPIASATRQIVAAAVGSGR
                     GDDEDFATLLLEVARGAGIALTPENVSVDDGLTPVRS"
     gene            971..2236
                     /locus_tag="VFC01_00705"
     CDS             971..2236
                     /locus_tag="VFC01_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZN78197.1"
                     /translation="MTDLLAPPVTARPLGAPGHMGVDYEERVDFARLRDYRIGRAKAA
                     LEASGCGAFLLFDFYNIRYTTQTWIGGALGDKMIRYCLLTRDGKPILWDFGSAVRHHK
                     LYSPWLPQENWRPGFLGFRGAVAPDAGLMRDAVSEIKGILADAGVAGAPVGLDIVEPP
                     FLFEMQRQGLTVVDAQQSMLDARVIKSADEIMLLNQAAAMVDGVYQDIVEVLKPGVRE
                     NEIVALANKRLYEMGSDQVEAVNAISGERCNPHPHNFTDRIIRPGDQAFFDIIHSYNG
                     YRTCYYRTFGVGSATQSQRDAYKQAREWMDTSIAAIRPGVGSDQIARLLPKATDFGFE
                     NEMAAFGLQFAHGLGLGLHERPIISRLNSLENPIELKAGMVFAMETYAPASDGFSAAR
                     IEEEVVVTDDGPVILTKFPAQELFVANEY"
     gene            complement(2300..3676)
                     /locus_tag="VFC01_00710"
     CDS             complement(2300..3676)
                     /locus_tag="VFC01_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016889681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinic semialdehyde
                     dehydrogenase"
                     /protein_id="HZN78198.1"
                     /translation="MAIATVNPTTGETVETFQTHDSAEVERRIAQATEAAVALHDTTF
                     AQRAEWMNATADILEADVERAAHMITLEMGKPIGQARAEVLKCAKNMRFYAAHAESFL
                     ADELLADPSAVAASQAGSVWQPLGVVLAVMPWNYPLWQVIRFAAPALMAGNTGLLKHA
                     SNVPHSALYLDELFDKGGFPPGCFRTLLIGSAEVAAVIEDERVKAVTLTGSEPAGRSV
                     ASSAGREIKKAVLELGGSDPFIVMPSADLDTASTVAVKARVSNNGQSCIAGKRFIVHA
                     DVYDAFVRLFVDKMSALVVGDPMDEATDVGPLATESGRDEIAELVDDAVAKGAKVLTG
                     GSTPQRPGWFYSPTVLVGLTEDMRVVMEETFGPVASVYKVADREEAVRVANQTTFGLS
                     SAVWSTDAAEQDWFIDHLDAGAVFLNGMTVSYPELPFGGVKASGYGRELASAGIREFC
                     NLKTVWKA"
     gene            complement(3795..4454)
                     /locus_tag="VFC01_00715"
     CDS             complement(3795..4454)
                     /locus_tag="VFC01_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003943535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN78199.1"
                     /translation="MAQSPVRDGITGARVADELRDAILRGAHPPGTRLRQEELAVQYG
                     ASRVPVREALRILESEGLVTTVANAGAWIARLSLDECVELYQVRERIEPLLLRYSLPH
                     LTAEQIERLADLVEEMRQSRDVERFLVLDREFHLGSYAGAETTFLGPTVERLWNTTQH
                     YRRAYTRLLDGESNRILHDEHHMLVAAIRDSDSDEAERVLFGHIRRTRLQLARHPEVF
                     A"
     gene            complement(4479..>5056)
                     /locus_tag="VFC01_00720"
     CDS             complement(4479..>5056)
                     /locus_tag="VFC01_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN78200.1"
                     /translation="VFGLLSLLLDPLEEDRAMALPGLVGTDHIGFTVPDLDEAHDFFV
                     RVLGCRHVYTLGPFEHHDDWMRDHLNVHPRTIMRELRFYRCGTGTNFEVFEYQPADGQ
                     APIPRNSDIGGHHLAFYVDNLDAAVEYLHSEGIRVLGEPTASRNASEGQRWVYFLSPW
                     GMQFELVSFPDGKAYEKDAEVLLWNPNHPAQ"
BASE COUNT          861 a         1635 c         1650 g          910 t
ORIGIN      
        1 aacggcctga ctcaacagaa aaggattcaa ctatgtccgc ctcaaccttg aagatcggct
       61 ggatcggtgc cggccgcatg ggagcgcagc tcgttacccg gctcctcgac gccgggtacg
      121 acgtcacggt gtacaaccgc accgcctcca aggcccagca cctagccgac cgcggagccg
      181 gaattgtcag ccgcccagtc gatctcgccg accgcgattt ggtgttcagc atggtgtcct
      241 cgtccaagga cctcgagcag gtcatgctcg gcgagggtgg cctgctcacg ggagccgcgt
      301 cgccgcgcgt gatcgccgac gtgtcaacgg tgtccgctga cgcgagcgct ctcatccgcg
      361 aggccgcgaa ctcgcgcggt tccgattttc tcgctacacc ggtcagcggc aacccgaagg
      421 tgatcgccgc gggcaagttg accgtggcgg tgtcggggcc ccgcgaggtg ttcgaatcga
      481 tcgagccggt gctgaacgtg tttggccgca gcgtcaccta cgtcggtgag ggcgaagtgg
      541 cgcgactggt gaagatcgca cacaacctca tgctcggcgt ggtgacccag tgccttgccg
      601 agatcaccgt gctcgtggag aagggcggcg tcagccgcgc cgacttcctc gcctttctca
      661 acgactcggt gatgggatcg gtattcaccc agtacaagtc gcccgcgttt gtcaacctcg
      721 acttcacccc gacctttacg atgcagctgt tacagaagga tttcgacctg ggtctcgagg
      781 ctgccaatca gctcaagtcg ccgatgccga tcgcgtcggc tacgcgccag atcgtcgctg
      841 ccgcagttgg atccggtcgt ggcgacgacg aggacttcgc cactctgctg ctggaggttg
      901 cccgcggcgc cgggatcgcg ttgacgccgg agaacgtctc ggtggacgac ggcctcacgc
      961 cggtgcggtc atgacggatc tgttggcccc gccggtcacc gcgcgcccac tgggcgcgcc
     1021 cggtcacatg ggtgtggact acgaagagcg ggtcgacttc gcccggttgc gcgactaccg
     1081 gatcggacgg gcgaaggcgg cgttggaggc cagcggctgc ggcgcgttct tgttgttcga
     1141 cttctacaac atccgttaca ccacgcagac gtggatcggt ggtgccctcg gcgacaagat
     1201 gatccgctac tgtctgctga cacgcgacgg caaaccgatc ctgtgggatt tcggttccgc
     1261 ggtgcggcac cacaagctgt actcgccgtg gctgccgcag gagaactggc ggcccgggtt
     1321 tctggggttc cgtggcgcgg tggctcccga cgcggggttg atgcgcgatg ccgtctccga
     1381 gatcaagggc attctcgccg atgcgggtgt ggctggcgcg ccggtgggac tggacatcgt
     1441 cgagccgccg ttcctgttcg agatgcagcg ccaggggctg accgtcgtcg atgcccagca
     1501 gagcatgctg gacgcccggg tgatcaagtc cgccgacgag atcatgctgc tcaaccaggc
     1561 ggccgccatg gtggatggtg tctaccaaga catcgtcgaa gtgctcaaac ccggtgtgcg
     1621 cgagaacgag atcgtcgcgc tggccaacaa gcggctgtac gagatggggt cggaccaggt
     1681 cgaggccgtc aacgcgatat cgggtgagcg gtgcaacccg cacccgcaca atttcaccga
     1741 ccgcatcatc cggccgggcg atcaggcgtt cttcgacatc atccactcgt acaacggcta
     1801 tcggacctgc tactaccgca cgttcggggt cggcagcgcc acgcagagcc agcgcgacgc
     1861 ctacaagcag gcccgggagt ggatggacac ctcgatcgcg gcgattcggc ccggtgtcgg
     1921 ctcggaccag atcgctcgat tgcttcccaa ggcaaccgat ttcggcttcg agaacgagat
     1981 ggcggcgttc gggctgcagt tcgcgcacgg cctcgggctg ggccttcatg agcggccgat
     2041 catctcgcgg ctgaactcgc tggagaaccc gatcgagctc aaggccggca tggtgttcgc
     2101 gatggagacc tacgcgcccg cctccgacgg cttctcggcg gcacggatcg aggaggaggt
     2161 cgtggtcacc gacgacggtc cagtgatcct gaccaagttc ccggcgcagg agttgtttgt
     2221 cgccaacgag tactgaacag atacaaatca gcccggcgcg atgcggcgcc ggtctgtttt
     2281 gtgtcgtggc tcaggccttt taggccttcc acaccgtctt gaggttgcag aactcccgga
     2341 tgcccgcgct ggccagctct cgcccgtagc ccgacgcctt taccccgccg aacggcagct
     2401 cggggtagga caccgtcata ccgttgagga acacggcacc ggcgtcaaga tgatcaatga
     2461 accaatcctg ttctgctgca tcggtgctcc acaccgcaga gctcaagccg aacgtcgtct
     2521 ggttggcgac gcgcaccgct tcctcgcgat cggcgacctt gtaaacggag gccaccgggc
     2581 cgaatgtctc ctccatcacc acccgcatgt cctcggtgag gccgacgagc acggtggggg
     2641 aatagaacca ccccggtcgc tgcggcgtcg aacctccggt gagaaccttc gcgcccttgg
     2701 cgacagcgtc gtcaacgagt tcggcgatct cgtcgcgtcc cgattcggtg gcaagcggcc
     2761 cgacgtccgt ggcctcatcc atcgggtcgc ctaccaccaa agcgctcatc ttgtcgacga
     2821 acaggcgcac gaacgcgtcg tagacgtcgg cgtgcacgat gaatctcttt ccggcgatgc
     2881 aggattggcc gttgttggag acgcgggcct tcacggccac ggtggacgcg gtgtccagat
     2941 ccgcggacgg catcacgatg aacgggtcag atccaccaag ttcaaggacc gccttcttga
     3001 tctcccggcc cgccgaggac gccaccgagc ggcctgccgg ctccgagccg gtgagcgtga
     3061 ccgccttgac ccgttcgtct tcaatcaccg cggccacctc cgccgagccg atcagcagcg
     3121 tgcgaaagca gccgggcggg aatcctccct tgtcgaagag ctcgtcgagg tacagcgcgg
     3181 agtgcggcac attggacgcg tgcttgagca ggccggtgtt acccgccatc aatgcgggtg
     3241 ccgcgaaccg gatgacttgc cacagcgggt aattccacgg catcacggcc agcacgacac
     3301 cgagcggctg ccacaccgag ccggcctgcg acgcggcgac ggccgacggg tcggccagca
     3361 gttcgtcggc aaggaacgac tcggcgtgcg cggcatagaa gcgcatgttc ttcgcgcact
     3421 tgagcacctc tgcgcgggcc tggccgatgg gcttccccat ctcgagggtg atcatgtggg
     3481 cggcacgttc gacatcggcc tcgaggatgt cggcggtggc gttcatccac tccgcacgct
     3541 gggcgaaggt ggtgtcgtgc aacgcaactg cggcctcggt cgcctgggcg atccggcgct
     3601 ccacctcggc gctgtcgtgg gtttggaagg tctcgacggt ttcgccggtg gtcggattga
     3661 cggttgcgat ggccatgtgc gcgatccctg attcgacgga gcgttctgca ctgattggat
     3721 cctatttcgg gtcaatgctc ggcaaaacag cggacaatgg ccagtctagc cgaccgattg
     3781 gatgcaatca acggtcacgc gaagacctcc gggtggcgtg cgagctgcag cctcgtgcgc
     3841 cggatgtgcc cgaacagcac ccgctccgcc tcatcggaat cgctgtcgcg gatcgcggcg
     3901 acgagcatgt ggtgctcgtc gtgcaggatg cggttgcttt ccccgtcgag aaggcgtgtg
     3961 tatgcccggc ggtaatgctg ggtggtgttc cacagccgct cgacggttgg gccaaggaac
     4021 gtcgtttccg caccggcata actgccgagg tgaaactcgc ggtcgagtac gagaaaccgc
     4081 tcgacatccc ggctttgacg catctcctcg acgagatcgg cgagccgctc gatctgctcc
     4141 gcggtgaggt ggggcaggct gtaacgcaac agcaggggtt cgatgcgctc gcgcacttgg
     4201 tacagctcaa cgcactcgtc gaggctgagg cgcgcgatcc aagcgccggc gttggccacg
     4261 gtcgtgacga ggccctcaga ttccaggatg cgcagcgctt cccgcaccgg aactcggctc
     4321 gcgccgtact gcaccgcgag ttcctcctgg cgcagccgcg tgcccggcgg gtgcgccccc
     4381 cgcaggatcg catcgcgcag ctcgtcggcg acccgcgcgc cggtgatccc gtcgcgcaca
     4441 ggggattgcg ccatcaccgc ctccgatccg ttaccgactc actgcgccgg gtggttcggg
     4501 ttccacagca gcacctcggc gtctttctcg taggccttgc cgtccgggaa gctcaccagc
     4561 tcgaactgca tgccccacgg gctgagaaag tacacccatc gctggccttc gctcgcgttg
     4621 cgactggccg tcggttcgcc gagcacacgg atgccctcgg agtgcaggta ttccacggca
     4681 gcgtcgagat tgtcgacata aaaggcaagg tggtggccac cgatgtcgct gttgcgcgga
     4741 atgggggcct gcccatcggc cggctgatac tcgaagactt cgaaattcgt tccggtgccg
     4801 cagcgataga accgcagctc gcgcatgatc gtgcgcgggt gaacgttgag gtggtcgcgc
     4861 atccagtcgt cgtgatgctc gaacgggccc agcgtgtaga cgtgcctgca gccgagcact
     4921 cgcacgaaga agtcgtgcgc ctcgtccagg tcgggcaccg tgaatccgat gtgatcggtg
     4981 cctacgagcc ccggtaacgc cattgccctg tcctcctcaa gtggatccaa taacagcgat
     5041 agtagcccga acacca
//
LOCUS       DASRJA010000017         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_825023,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000017 DASRJA010000000
VERSION     DASRJA010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825023"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFC01_00725"
     CDS             complement(<1..402)
                     /locus_tag="VFC01_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78201.1"
                     /translation="MRIRGVSLCVGAVGIVLAVYAHLNAQQKTAVRAFTDGDWPRYAG
                     DFAGTKYSKLKQIDASNVSKLVAAWTFEGVGAEQTPIVVDGVMYASTSTGVVALDADT
                     GKLIWRYGAVPPAGGGGGRRGRGAPGGAGPGG"
     gene            complement(426..1214)
                     /locus_tag="VFC01_00730"
     CDS             complement(426..1214)
                     /locus_tag="VFC01_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78202.1"
                     /translation="MGSRSARFAVVAMVMMLPAVTAAQYLKHRDPKIPRTADGKPNLT
                     APAPRTADGKPDLSGLWNAVDGRFLSNISQRAGVTAPFTPWAAALFKERQENQGRDRP
                     AGFCLPHTVPTAMLVPNYPWKVVQTPGLTIILFENFTDFRQIFTDGRDFPEERAPTWF
                     GYSLGRWDGDTFVVNTLGFNDKAWLDDGGHPRSEEMKVTERFRRKDVGHLEIEFTFDD
                     PKAYTRPWSATVPFELIPDSEIIENICENEQDIKHIFFDQKNAK"
     gene            complement(1279..2112)
                     /locus_tag="VFC01_00735"
     CDS             complement(1279..2112)
                     /locus_tag="VFC01_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HZN78203.1"
                     /translation="MRRLISRQVIGWLVAGGCIATVTGSCIATVASAQQTAPAAPQGQ
                     QTAPAAPQGAPQGAALPGGRGGPAAPATVPFENTRNAARHQGFLEVAKAGNIDLLFVG
                     DSITDGWRNRGLEVWNKYFEPLKAANFGIGGDTTQGVLWRMQNGELEGFKAKLIILML
                     GTNNINRNPNADIAEGDRLIVEEFKKRQPQAKILILGIFPRAVEPTSPLRPAIKEING
                     MLAKLADNRQVFYMDIGDKFLAPDGRLTPEIMPDALHPNLAGYQIWADATSDRVKELM
                     K"
BASE COUNT          385 a          800 c          742 g          435 t
ORIGIN      
        1 accccccgga cccgcgccac ctggcgcgcc gcggccgcgg cggccaccgc cgcctcccgc
       61 aggcggcacg gcgccgtacc gccagatcag cttgcccgtg tccgcgtcga gggccaccac
      121 cccggtcgac gtcgacgcgt acatcacccc gtccacgacg atcggcgtct gctcggcccc
      181 gacgccctcg aacgtccagg ccgccaccag cttcgagacg ttcgacgcat cgatctgctt
      241 cagcttcgag tacttcgtcc ccgcgaagtc gccggcatag cgcggccagt cgccgtcggt
      301 gaaggcgcgc acggcggtct tctgttgcgc gttcaggtgt gcgtacaccg cgagcacgat
      361 accgacggcc ccaacgcaca gagagacacc tcgaatccgc atcagccgat cctttcgctc
      421 gtcaactact tggcgttctt ctgatcgaag aagatgtgct tgatgtcctg ctcgttctcg
      481 cagatgttct cgatgatctc gctgtcgggg atcagctcga acggcacggt ggcgctccac
      541 ggtctggtat acgccttggg atcgtcgaac gtaaactcga tctcgaggtg accgacgtcc
      601 ttccgccgga agcgctcggt caccttcatc tcctcgctcc tcggatgccc gccatcgtcg
      661 agccacgcct tgtcgttgaa gccaagggtg ttgacgacga aggtgtcacc gtcccagcgg
      721 ccgagcgagt agccgaacca ggtcggcgcc cgttcctcgg ggaagtcgcg gccgtcggtg
      781 aagatctgcc ggaagtcggt gaagttctcg aagaggatga tcgtgagacc cggtgtctgc
      841 acgaccttcc aggggtagtt cggcaccagc atcgcggtag gcaccgtgtg cggcaggcag
      901 aatccggctg gacggtcgcg cccctggttc tcctgccgct ccttgaagag cgccgccgcc
      961 cacggcgtga acggcgcggt cacgccggcg cgctgcgaga tgttggagag gaagcgtccg
     1021 tcgacggcat tccacaaccc actgaggtct ggcttgccgt cggcggtgcg cggcgccggc
     1081 gccgtgagat tgggcttgcc gtcggccgtc cgcggaatct tcgggtcgcg atgcttgagg
     1141 tactgcgcag cagtgaccgc cggcagcatc atcaccatcg cgacgaccgc gaagcgagcg
     1201 ctcctactac ccacgaagct cctatccatt gggaacccac ctccgccaag gctacggtgg
     1261 gcaagcaatc cgagcgacct atttcatcag ctccttgacc cggtcactcg tggcatcagc
     1321 ccagatctga taaccggcaa ggttcgggtg cagggcgtcg ggcatgatct cgggtgtcaa
     1381 ccggccatcg ggcgcgagga acttgtcgcc gatatccatg tagaacacct gacggttgtc
     1441 ggccagcttg gccagcatgc cgttgatctc cttgatggct gggcgcaatg gactggtcgg
     1501 ctccaccgcc ctcgggaaga tgccgagaat caggatcttg gcctgcggct gccgcttctt
     1561 gaactcctcg acgatcagcc ggtcaccttc cgcgatgtcg gcgttcgggt tgcggttgat
     1621 gttgttggtg ccgagcatga ggatgatgag cttcgccttg aagccctcga gctcgccgtt
     1681 ctgcatccgc cagaggacgc cctgcgtggt gtcgccgccg atgccgaagt tggcggcctt
     1741 cagcggctcg aagtatttgt tccacacctc gagcccgcga ttgcgccagc catcggtaat
     1801 cgaatcgccg acgaagagca ggtcgatgtt gcctgccttg gctacctcga gaaagccctg
     1861 atgccgcgcg gcgttgcggg tgttctcgaa cggcacggtg gccggcgccg ccggtccgcc
     1921 gcggccgcca gggagggccg cgccctgcgg cgcaccctgc ggggctgccg gcgccgtctg
     1981 ctgcccctgc ggggctgcag gagccgtctg ctgagcactc gccaccgtgg cgatgcacga
     2041 gcccgtcacc gtggcgatgc acccgcccgc caccagccac ccgatgacct gtcgagatat
     2101 cagtcggcgc atctctgttt ctccttgctg tgcttcatct gaacgggacg gcgaaagatt
     2161 actccagggg cggcagaaag cgcacacggc ctctgcggtc ccggatccgg gcccggaatc
     2221 cgcccatcac gacgacgcat cgacacgcag accgactcgc gggcactccg catttgacgg
     2281 gcggcgcagt gtgtgttatt tttgtcgcac gttgatgcga gacaaataac acatgagcaa
     2341 atcccttcac accgcgttgg cc
//
LOCUS       DASRJA010000018         6420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1118180,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000018 DASRJA010000000
VERSION     DASRJA010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118180"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..517)
                     /locus_tag="VFC01_00740"
     CDS             complement(20..517)
                     /locus_tag="VFC01_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZN78204.1"
                     /translation="MVLLGWAVRNGSTALDDWFHRYGHSPAHRLLFFTDSWVLVLIVA
                     ATLAVSLYRRQWRLAIATAVCPLLAMGLVRLMKPLFDRRSSGALAYPSGHTATVVVVM
                     GMVVLVAGAALWAVLVAVGWCLLGMVGQAVTYHYFTDTVGALLLGTAIVCVAALVLRR
                     VAHQT"
     gene            complement(550..1065)
                     /locus_tag="VFC01_00745"
     CDS             complement(550..1065)
                     /locus_tag="VFC01_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol transferase"
                     /protein_id="HZN78205.1"
                     /translation="MADSDAAAVRELLRDSFTRLIEHVEDLTDGLTDEISFFQPTASA
                     NSISWLIWHSARQHDAQLCDIAGIEQVWTREGWVDRFGLDLPRDAHGYGHTAEDVGKV
                     RAPADLLAGYYHAVHKVTLEYIASVSAEELNRVVDTNWNPPVTASVRLVSIIDDSAQH
                     LGQAAYVRGIA"
     gene            complement(1117..1956)
                     /locus_tag="VFC01_00750"
     CDS             complement(1117..1956)
                     /locus_tag="VFC01_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZN78206.1"
                     /translation="MLYLPPAWYASNPPPRLPAVVMIHAEFGHPNDWLVGGHARKTLD
                     DFAAAHGGNAPVVVFADASGTFSNDTECVNGLRGNAADHITKDVVPFVISNFGVSRDP
                     ANWGIAGWSAGGTCAVTLAVKYPEIFSTFVDIDGQKGPYAGTPEQTVARLFGGDTAAW
                     ADFDPRTIMERHGPYTGVSGWFGVSTENPTVYRDGSSNVGAELPPEPDAKTNATDPTA
                     VANYMCELVSIYGIECAVVPQIGGHNFVNAGAQFEKALPWLAGQLGTPGVPKIVPPGA
                     PAS"
     gene            complement(1969..2280)
                     /locus_tag="VFC01_00755"
     CDS             complement(1969..2280)
                     /locus_tag="VFC01_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78207.1"
                     /translation="MARRLRRLNRVICVALLATAAAVGCQNAPGQSNRIDVAGAKLPG
                     DFGGSDPGTLVSANLLQNLGAPLSNQTDQAGVSEMQRTGAAPSKGTMVSVDIPDDASG
                     F"
     gene            complement(2283..2663)
                     /locus_tag="VFC01_00760"
     CDS             complement(2283..2663)
                     /locus_tag="VFC01_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN78208.1"
                     /translation="MIDHFGINCSDFSKSKEFYDRVLGVLGYTRQMDYEVAIGYGRDG
                     KPDFWIAECRETGPNREIHVAFRADNIGAVKAFYDSALALGAESLHAPRLWPEYHPGY
                     FGAFVRDPDGNNVEAVFHGATAGG"
     gene            2716..4446
                     /locus_tag="VFC01_00765"
     CDS             2716..4446
                     /locus_tag="VFC01_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN78209.1"
                     /translation="MAYELVIRNGTVIDGLGREPYVGNVAVSGGAIAAVGVVDGTGQR
                     EIDATGLLVTPGFIDLHTHYDGQAIWSDRMTPSSAHGVTTAVMGNCGVGFAPCRREDH
                     EVLVDVMAGVEDIPGVVMVDGLPWDWETFPEYLDALDSRRRDIDVAAYLPHSPLRVYV
                     MGQRGADREPATDEDLKRMRALAKEAVEVGALGFASSRFSFHKTASGELIPTYNAAQA
                     EIDAIASGVKDGGGGLIQFVPDIAAGGYANVLQQVFEAAQDNGLPVTFTLATGNTGDP
                     IWPDAITLIEKYNSVGDPDTAITAQMFPRPIGLVIGLELTGNPFVFYPSYREIADLPL
                     AERVAEMRKPEVRERILADKPADGGHPLLYLAQAWDWIFPLTDDPKYEPDASTSIAAR
                     ARARGVSPMEEAYDRLLDDDGHAMLLVAMANFENNSLDTVGELIRRDDVVLGLGDGGA
                     HYGMICDASYPTFLLAHWARDRQKQRLSVPDAIRMMTSVPARVAGLADRGRIAVGYKA
                     DLNVIDHAALRLHKPVITHDLPAGGRRLDQTADGYRATIVSGEIIAENGVPTAARPGR
                     LVRGRQPAPA"
     gene            4462..4833
                     /locus_tag="VFC01_00770"
     CDS             4462..4833
                     /locus_tag="VFC01_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZN78210.1"
                     /translation="MSVKVDLDQLADALADFTFAYLITVGDDYRAHTVAVGPVFADGV
                     IDVGSIGDSTRRNVTQHDGVTLVWPPREPGGYTLIVDGLGRPTDAGLTVEPTRAVLHR
                     PAAPGAATKPGCLHDCVPLES"
     gene            complement(4959..>6420)
                     /gene="groL"
                     /locus_tag="VFC01_00775"
     CDS             complement(4959..>6420)
                     /gene="groL"
                     /locus_tag="VFC01_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214954.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZN78211.1"
                     /translation="AKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALV
                     REGLRNVAAGANPLGLKRGIEKAVEKITETLLKSAKEVETKEQIAATAAISAADTQIG
                     ELIAEAMDKVGNEGVITVEESNTFGLQLELTEGMRFDKGYISGYFVTDAERQEAVLED
                     PYILLVSSKVSTVKDLLPLLEKVIQSGKPLLIIAEDVEGEALSTLVVNKIRGTFKSVA
                     VKAPGFGDRRKAMLQDIAILTGGQVVSEEVGLSLETADVSLLGQARKVVVTKDETTIV
                     EGAGDSDAIAGRVAQIRSEIENSDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKE
                     RKHRIEDAVRNAKAAVEEGIVSGGGVALLQAAPALDDLNLEGDEATGANIVRVALEAP
                     LKQIAFNSGLEPGVVAEKVRNSAAGTGLNAQTGVYEDLLKAGVADPVKVTRSALQNAA
                     SIAALFLTTEAVVADKPEKAPAGAVDPTGGMGGMDF"
BASE COUNT         1082 a         2147 c         2054 g         1137 t
ORIGIN      
        1 gcaccggcgt tgacacctgt caagtttggt gggctacgcg tctcaatacg agggccgcga
       61 cgcacacgat cgccgtgccc agcagcagcg cacccacggt gtcagtgaag tagtggtaag
      121 tcacggcctg tcccaccatg ccaagcaggc accaaccgac cgcgacgagc accgcccaca
      181 gcgctgcgcc cgccaccagc acgaccattc ccatcacaac gaccaccgtc gctgtatgtc
      241 cgcttggata agccaaggcg ccgctgctcc gacggtcgaa caacggcttc atcagccgta
      301 cgagccccat cgcaagcagc gggcacaccg ccgtcgcgat cgccaatcgc cactgccgtc
      361 ggtacagcga gacggccaga gtcgccgcaa caatcagcac cagaacccac gagtcggtga
      421 agaacagcag ccggtgcgca gggctatgcc cgtagcgatg gaaccaatcg tccaacgccg
      481 tcgagccgtt tcgcaccgcc caacccagca gcaccatgcc cgtcagcgcg actggcggcc
      541 aggggcggat caggcgatac ctcgcacgta cgccgcctga ccgaggtgct gcgcggaatc
      601 atcgatgatg ctgaccaacc gcacgctagc cgtgacgggc ggattccagt tcgtgtcgac
      661 gacgcggttc agctcctcgg ccgacaccga ggcgatgtat tccagcgtca ccttgtgcac
      721 cgcgtggtag tagccggcga gtagatcggc cggcgcgcga acctttccca cgtcttcggc
      781 ggtgtggcca tacccatgcg catcacgcgg caggtccagc ccgaaccggt cgacccaacc
      841 ttcgcgtgtc cacacctgtt cgatgcccgc gatatcgcag agctgggcgt cgtgctggcg
      901 ggcgctgtgc cagatcaacc acgaaatgct attggcggag gcagtgggct ggaagaacga
      961 gatttcgtcc gtcaatccgt cggtcaagtc ctcgacgtgc tcgatgagcc gggtgaacga
     1021 gtcacgcagt aattctcgga ccgccgccgc atcagagtca gccatgcctt gacgctaccc
     1081 gcggacgatg acgccccttg gctggcctgc tcggcgtcag ctagccgggg cgccgggcgg
     1141 cacaatcttc ggcacacctg gggtaccgag ctgtccggcc agccacggca gtgccttctc
     1201 gaactgggct ccggcgttca caaagttgtg cccgccaatc tgcggcacca ccgcgcactc
     1261 gatgccgtaa atgctgacca gctcgcacat gtagttcgcg actgcggtcg gatcggtcgc
     1321 attggttttg gcgtccggct ccggtggcaa ttcggcgccg acgttcgacg agccgtctcg
     1381 gtagacggtc gggttttccg tcgagacgcc gaaccacccc gatacgcctg tgtaaggccc
     1441 gtgcctttcc atgatcgttc tcggatcgaa gtcggcccac gccgcggtat caccgccgaa
     1501 cagtcgcgca acggtctgtt cgggcgtgcc cgcgtacggt cccttctggc cgtcgatgtc
     1561 gacaaaggtg ctgaatatct cggggtactt caccgccaac gtaaccgcgc acgtgccgcc
     1621 tgccgaccat ccggcgattc cccagttcgc cgggtcccgg ctcactccga agttcgagat
     1681 cacaaacggc acaacgtctt tggtgatgtg atcggcagca ttcccacgca agccgttgac
     1741 gcattcggtg tcgttgctga acgtcccgct cgcgtcggcg aagaccacaa ccggtgcgtt
     1801 accgccgtgc gcggcggcaa aatcgtcgag cgtctttcgt gcgtgtcccc ccaccagcca
     1861 gtcgtttgga tgcccgaact cggcgtgaat catcaccacc gccggcaacc ggggcggcgg
     1921 attgctggcg taccaggcag gcggcaggta cagcaactcc ttgcggtgtt agaaccccga
     1981 cgcgtcgtcg ggaatgtcaa ccgacaccat ggtgcccttg ctgggcgccg cacccgtgcg
     2041 ctgcatttcg gaaacaccgg cttggtcggt ctgattggac agcggggcgc cgaggttttg
     2101 caacaggttt gccgagacaa gcgtgccggg gtcagaaccg ccgaagtcgc cgggcagctt
     2161 tgcgccggcg acgtcgatgc ggttcgactg acccggtgcg ttctggcacc ccacagctgc
     2221 cgccgtggcg agtagcgcca cgcagatcac ccggttgagc cgcctcagcc gtcgcgccat
     2281 ggctagccgc ccgctgtggc gccatggaat acggcctcca cgttattgcc gtccgggtcg
     2341 cggacgaatg cgccgaagta gccggggtga tactccggcc acaaccgcgg cgcgtgcagg
     2401 gactcagcgc ccagtgcaag cgcagagtcg tagaaagcct ttacggcgcc gatgttatcc
     2461 gcccggaaag caacatgaat ttcccggttt gggcccgttt ccctacactc tgcgatccag
     2521 aagtcgggtt tgccatctcg gccgtagcct atagccacct catagtccat ctgccgcgtg
     2581 tagccgagca cgccgagcac gcggtcatag aactcttttg atttgctgaa gtcactacag
     2641 ttaattccaa agtggtcgat caccggtaca tcctggcatg gtttctcttg agcaactctt
     2701 cggtacattc agttgatggc gtatgagtta gtcatccgta acggcaccgt aattgacggt
     2761 ctcggccgcg agccctacgt cggcaacgtc gcggtctccg gcggcgccat tgccgccgtc
     2821 ggcgtcgtgg acggtaccgg acaacgcgag atcgacgcga ccgggctgct cgtcaccccc
     2881 gggttcatcg acctacatac tcattatgac gggcaggcca tctggtcgga ccgcatgacg
     2941 ccgtcgtcgg cgcacggggt gacgaccgcg gtgatgggca actgcggcgt ggggtttgcg
     3001 ccatgtcgcc gcgaggacca cgaggtgctc gtcgacgtga tggcgggtgt cgaggacatc
     3061 cccggcgtgg tcatggtcga cggcctgccg tgggattggg agacgttccc cgagtacctg
     3121 gacgcgttgg actcacgacg tcgggatatc gatgtggcgg cgtacctgcc gcactcgccg
     3181 ctgcgggtat acgtgatggg ccagcgcggc gctgaccgcg agccggcgac cgacgaggac
     3241 ttgaaacgga tgcgggcgtt ggccaaggag gcggtcgagg tcggggcgct aggattcgcg
     3301 tcgtcgcggt tctcatttca caagaccgcc agcggcgagc taattcccac ctacaacgct
     3361 gcacaggcgg agatcgacgc gatcgcctcc ggtgtcaagg acggcggcgg cggactgatt
     3421 caattcgtgc ccgacattgc ggcgggcggt tatgccaatg tgctgcagca ggttttcgag
     3481 gcggcccagg acaacgggct gcctgtgacg ttcacgttgg cgacgggcaa tacaggcgat
     3541 ccgatctggc ccgacgcgat cacgctgatc gagaagtaca actcggttgg cgacccggac
     3601 accgcgatca cggctcaaat gttccctcgc ccaattggtt tggtcatcgg tctggagctg
     3661 acaggcaacc cattcgtgtt ctacccgagc taccgggaga tcgcggatct gccgctggcg
     3721 gagcgcgtgg ccgaaatgcg caaacccgaa gtgcgcgaac gcatcctcgc cgacaagccc
     3781 gctgacggtg gccatccgct gctgtatctg gcacaggcat gggactggat tttcccgctg
     3841 accgatgacc cgaagtacga gccggacgcg tcgacctcga tcgcggccag ggcccgggcc
     3901 cgcggcgtca gccccatgga ggaggcctac gaccggctgc tcgacgacga cggccacgcc
     3961 atgttgctgg tcgcgatggc caacttcgag aacaactcgc tcgacacggt cggggaattg
     4021 atccgtcgag atgacgtcgt tctcggtctc ggcgacggcg gcgctcacta cggaatgatt
     4081 tgcgacgcaa gctatccgac gttcctgctg gcgcactggg ctcgcgaccg gcagaagcag
     4141 cggctcagcg tccctgatgc gattcggatg atgacgtcgg tgcccgcgcg tgtcgccggc
     4201 ctcgctgacc gcggccgaat cgcggtgggc tacaaggccg atctcaacgt catcgaccat
     4261 gcggcgttgc ggctgcacaa gccggtcatc acccacgatc tgcctgccgg cgggcgtcgc
     4321 ctggaccaga ccgcagacgg ctaccgggcg acgatcgtgt cgggcgagat catcgccgaa
     4381 aacggtgtgc ccaccgcggc gcgtcccgga cgccttgtgc gcggccgtca acccgcaccg
     4441 gcgtaagccc atggggccgt catgagtgtc aaggtggatc tcgaccaatt ggccgacgcg
     4501 ctggccgatt tcacgttcgc ctatctgatc acggtcggcg acgactaccg cgcgcacacc
     4561 gtggcggtcg ggccggtgtt cgcggacggt gtgatcgacg ttggttccat cggcgacagc
     4621 acgcgccgaa acgtcactca gcacgacggc gtcacgctgg tctggccgcc gcgtgaaccg
     4681 gggggttaca cgttgatcgt tgacggcctc ggcaggccca ccgatgccgg gctgactgtc
     4741 gagccaaccc gcgcggtgct gcatcgtccc gccgcgcccg gggctgccac aaagcccggc
     4801 tgtctacacg actgcgtgcc gctcgaaagc tagatgccgc ggcgagtgtg ggcttgtgtc
     4861 acgctccgcg cgaaaatgcg tgcgggtaac ccacgttcgg caggccgcta cgacaaaggc
     4921 ccggtcccac aagggaccgg gcctcgtcgt tggcgacttt agaagtccat gccgcccatg
     4981 ccgccggtcg ggtcgaccgc accagcgggc gccttctccg gcttgtcggc gacgaccgcc
     5041 tcggtggtca ggaacagcgc cgcgatggac gccgcgttct gcagcgccga acgcgtcacc
     5101 ttgaccgggt cggccacgcc tgccttgagc aggtcctcgt acacgccggt ctgagcgttc
     5161 aggccggtgc cggcagccga gttgcggacc ttctcggcga caacgcccgg ctccaggccg
     5221 gagttgaagg cgatctgctt cagcggagcc tcgagcgcca ccctgacgat gttcgcaccg
     5281 gtggcctcgt cgccctcgag gttgaggtcg tcaagcgcgg gagcggcctg cagcagagcg
     5341 acaccgccac cgctgacgat gccctcctcg acggcggcct tcgcgttgcg aactgcgtcc
     5401 tcgatgcggt gcttgcgctc cttgagctcc acctcggtgg cagcgccggc cttgatcacc
     5461 gcaacaccgc cggccagctt ggccaggcgc tcctgcagct tctcgcggtc gtagtcggag
     5521 tcgctgttct cgatctcgct gcggatctgg gccacccggc cggcgatggc gtcggagtca
     5581 ccggcgccct cgacgatggt ggtctcatcc ttggtcacca cgaccttgcg ggcctggccg
     5641 agcagcgaca cgtcggcggt ctcgagggac aacccgacct cttcgctgac gacctggcca
     5701 ccggtgagga tcgcgatgtc ctgcagcatc gccttgcggc ggtcaccgaa gcccggggcc
     5761 ttgacggcga cggacttgaa ggtgccgcgg atcttgttga cgaccagggt ggacagcgcc
     5821 tcgccctcga cgtcctcggc gatgatcagc agcggcttgc cggactgaat gaccttctcc
     5881 agcagcggca gcagatcctt gaccgtcgac accttcgacg agacgagcag gatgtacgga
     5941 tcctcgagga ccgcttcctg acgctcggcg tcggtgacga agtaacccga gatgtagccc
     6001 ttgtcgaagc gcatgccctc ggtgagctcg agctgcaggc cgaaggtgtt ggactcctcg
     6061 acggtgatga caccctcgtt gccaaccttg tccatggcct cggcgatcaa ctcgccgatc
     6121 tgggtgtccg cggccgagat cgcggcggtg gcagcgatct gctccttggt ctcgacctcc
     6181 ttggccgact tcagcagcgt ctcggtgatc ttctcgacgg ccttctcgat gccgcgcttc
     6241 aggccgagcg ggttggcacc cgccgcgacg ttacgcagac cctcgcgcac gagcgcctgg
     6301 gccagcacgg tggcggtggt ggtgccgtcg cccgcgacat cgtcggtctt cttggcgact
     6361 tccttgacca gctcagcgcc gatcttctcg tacgggtcct ccagctcgat ctccttggcg
//
LOCUS       DASRJA010000019         5612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3369010,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000019 DASRJA010000000
VERSION     DASRJA010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3369010"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..314)
                     /locus_tag="VFC01_00780"
     CDS             complement(120..314)
                     /locus_tag="VFC01_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78212.1"
                     /translation="MRTRIAALLIAGLAVAGALGTAGVASAKHGADDPAGHIRHSQGA
                     DDPAGHNRHGGGADDGPNHH"
     gene            534..1892
                     /locus_tag="VFC01_00785"
     CDS             534..1892
                     /locus_tag="VFC01_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN78213.1"
                     /translation="MGNAAAPPRIHAAVARFVVGSLAAIAVIIVGGFFVLRSVAIAEA
                     TGDTRERVRSEGRLVEAAGLSDGILRGEPEAIRRLDDLVTSQVLTDSIVRVKLWTEDG
                     TILYSDEPALIGTRFALGNEERALFASGDADAHLSDLNQPENRYERPQGKLLEAYTTV
                     HTPDGTALMFEIYQRFSSVSASGARLLAALAPPLMGGVVVLLLFQVPLAWTLARRLQR
                     GHHEREELLANAVEASTQERRRIASGLHDGVVQDLAGVAFGLAPLTAAAQRRGADAEA
                     EVLRAATATLRQGVRDLRTLLLEIHPPNLEAAGLRVALSDLLSPLQASGIATTLKVDE
                     MQQPEASVGHGQAAGGDAAGSAPADALVYRVAREAIRNAQTHAHPSAVSVSVMREPGR
                     TRLVVTDDGSGFALQERQRRGSEGHLGLSLLQDIVAQANGTLDVRSVPGAGTTVTLEL
                     PA"
     gene            1892..2551
                     /locus_tag="VFC01_00790"
     CDS             1892..2551
                     /locus_tag="VFC01_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN78214.1"
                     /translation="MIRVLLADDHGVIRDGLGRLISALDGVTLVGVAADGAQAVEQHA
                     RLLPDVVLMDLDMRVMDGIEATKRIMAERPQTAVVVLTAFSDRPRIIGALDAGASGYL
                     LKDVDAEEVGEAIRAAARGESPLDPRVARTVLDAKPASGPLNRLSPRERQVLELLVEG
                     LPNKLIARRLLITEKTVKAHLTQIFRQLDVTDRTQAALWAARHGVGSDPNTAGTRDSQ
                     P"
     gene            2584..3185
                     /locus_tag="VFC01_00795"
                     /pseudo
     CDS             2584..3185
                     /locus_tag="VFC01_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705844.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3226..3355)
                     /locus_tag="VFC01_00800"
                     /pseudo
     CDS             complement(3226..3355)
                     /locus_tag="VFC01_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558251.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3459..4070)
                     /locus_tag="VFC01_00805"
     CDS             complement(3459..4070)
                     /locus_tag="VFC01_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78215.1"
                     /translation="MHVRYTLITADPERIDDAVAYLASDGRSRLESEPGAKGMSLQVS
                     PDLAVAVLESFWVSHDAMRESERVEAGVRDEAIRLALGTASVERFRRASVTRVGREKG
                     GAGVRHTVYQTNPAALDEAIAGYEDTAVPWLTETDGLVSAALLVDHRSGRQLSQTIWR
                     DIDALVKSRSPAAAIRVDAVAATDSAVVALEEYGLVFRSIRTE"
     gene            complement(4082..4813)
                     /locus_tag="VFC01_00810"
     CDS             complement(4082..4813)
                     /locus_tag="VFC01_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZN78216.1"
                     /translation="MARILIVLSGSDHWTLSDGTRHPTGFWAEEFVEPHRTFRHAEVD
                     VDIATPGGVRPTVDQVSLAPDRAGGEERAAELRRYLESLEDELAAPMSVEHAADNLGN
                     YDAVFIPGGHGPMEDLPDCRPLGRILADLFDTGRVVAAVCHGPAGLLAGRREDGTWLF
                     AGRRLTAFTNEEERQAGLADRAKWLLETRLREEGADFDQGQPWSSHVVVDGRLVTGQN
                     PASSKQAAERTLAVLMAAQSPRSFE"
     gene            5063..5455
                     /locus_tag="VFC01_00815"
     CDS             5063..5455
                     /locus_tag="VFC01_00815"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HZN78217.1"
                     /translation="MNAHEFYRDVAERAMLSKGEAADLTRTVLEALAMRVSAGEVRHL
                     IRQLPEELADSVRWKTKGPERFDLDDLIMRVSSRTGLTKTETLTGVEAVLLTLREAVD
                     WKEFHDFMSQLPVEFTRLLPSPNPPRAV"
BASE COUNT          921 a         1849 c         1849 g          993 t
ORIGIN      
        1 tcgcccattg atctgacggt ttcagactgc ggtcgagcgg ctgcctggtg gcaggaagat
       61 tcgtcatcct cgccgcaacc ggatgtgcgg cgaggatgac gagagtcggc gctgtgccgt
      121 cagtggtggt tcggaccgtc gtctgcgccg ccgccgtggc ggttgtggcc cgcggggtcg
      181 tcggcgccct gtgagtgccg gatgtggccg gcggggtcgt cggcgccgtg cttggcgctt
      241 gccacgcccg cggtgccgag ggcgcccgcg acggcgagac cggcaatgag aagagcagcg
      301 atacgagtac gcatggtgtg ttcctttcgt atgaacgatg tgaaacaacc ctggcaaggt
      361 gatggtcgaa atcgccagtg gaccttcgtc gtgcgaccaa ggtcgtaaga ccaaggtcca
      421 actgcaggtc cggcggttcg tgccaggcgg catcacggcg gaactatcgt gggctcgtgc
      481 tggaccgatg caccacacgc tggcgccggc aacggcctcg tcggcgccag cgtgtgggaa
      541 acgcagctgc cccgcctcgc attcatgctg cggttgcacg gttcgtcgtc ggtagcctgg
      601 cggccatcgc cgtcatcatc gttggtggct tctttgtgct gcgcagcgtc gcgatcgccg
      661 aggccactgg tgacacgcgc gagcgtgtcc gctccgaggg acgtctcgtc gaggcagccg
      721 gactgagcga cggaatcctg cgcggcgagc ccgaggcgat ccgccgtctc gacgacctcg
      781 tcaccagcca ggtgctcacc gactcgatcg tgcgtgtgaa gctgtggaca gaagacggca
      841 cgattctgta ctccgacgag cccgcgctga tcggtacgcg cttcgcgctt ggtaatgagg
      901 agcgtgcgtt gttcgccagc ggtgacgctg acgcccatct cagcgacctc aaccagcccg
      961 agaaccgcta cgaacgcccg caaggcaagc tgctcgaggc ctacacaacc gttcacacgc
     1021 ccgacggcac tgcgctgatg tttgagattt accagcgctt cagttcggtc agtgcgagcg
     1081 gagcgcggct gctcgctgcg ctcgcgccgc cactgatggg cggtgtggtg gtcttgctcc
     1141 tgtttcaggt gccgctggcg tggacgttgg cgcggcgatt gcagcgtggc caccatgagc
     1201 gggaggagtt gctggcaaac gcggtcgaag cgtcgacgca agagcgccgg cgaatcgctt
     1261 ctggcctgca cgacggcgtg gttcaggatc tggcgggagt ggctttcggg ttggccccgc
     1321 tgaccgccgc ggcccagcga cgtggcgctg atgccgaggc tgaggtgctg cgcgcggcca
     1381 cggcgaccct gcgccagggc gtgcgtgatc tgcggaccct gctgctggaa attcacccgc
     1441 ccaatctcga ggcagcaggt ttgcgggtcg cgttgagcga tctgctgagt ccgctccagg
     1501 caagcggaat cgcgacgaca ctgaaggtgg atgagatgca gcaaccggaa gcatccgttg
     1561 gccatggcca agcggcgggc ggcgacgctg cgggttcggc ccctgccgac gcactcgttt
     1621 accgcgtggc gcgtgaggcc atccgcaacg ctcagactca tgctcaccca tcggcggtta
     1681 gcgtttcggt catgcgtgag cccggccgca cccgcctcgt ggtgaccgac gacggttcag
     1741 gcttcgcgct tcaggagcgc cagcgacgcg gcagcgaggg ccacctcggc ctgagcctgc
     1801 tgcaggacat cgtcgctcag gccaatggaa cgctggatgt gcgttcggtg cccggcgccg
     1861 ggacgacggt gacgttggag cttccggcct gatgattcgt gtgctgctcg ccgacgacca
     1921 cggggtgatc cgcgacggcc tgggccggct gatatcggcg cttgacgggg tcaccctcgt
     1981 cggcgtggcc gccgatggcg cccaggccgt cgaacaacac gcgcggctgc tacctgacgt
     2041 cgtactgatg gacctcgaca tgcgggtcat ggatgggatt gaggcgacca agcgcatcat
     2101 ggccgagcgt ccacagaccg cggtggtggt actgaccgcg ttttctgacc gcccacgaat
     2161 catcggcgcg ctggacgctg gggcgtctgg atacctgctc aaggatgtgg atgccgagga
     2221 ggtcggcgag gccatccgcg cggcggcgcg cggagagtcc ccactcgacc cgcgtgtcgc
     2281 gcgtaccgta cttgacgcca agccggcgtc gggtcctctc aaccgcctct cgccgcgcga
     2341 gcgccaggtg ctcgagttgc tcgtcgaggg tctgcccaac aagctcatcg cacgacgcct
     2401 gctgatcacc gagaaaaccg tgaaagcaca cctgacgcag atcttccggc aactcgatgt
     2461 gaccgaccgc acgcaagccg cgctgtgggc cgctcgccac ggcgtcggga gcgacccgaa
     2521 taccgcaggc acacgcgatt ctcagccgta gcggccgcac catcggatgc gcgggcgggc
     2581 ctggtcgcgg tcgcccgcaa agccaccggg aaagctgctc gggcgcttga tttggaaccg
     2641 agcaaccgcg tcgaactagg cttcctgctc atcgccggcg tcgccgcgtt cctcatcctg
     2701 gcgaccggac agatccacct catcttcggc atcgcgctgc tggcctggtt cggcttctac
     2761 ctatacaaac tgtcccgcgg cgacgtcgaa gaacccgacc tgatcggcgc ggccgccgcg
     2821 ctcggcgagc tgcccaaccg cgcccgacgg gtcaccgtcg tcgcgatgtt cgtcgtcgcg
     2881 gccgcgatca tcctcgcgtg cgcaaaaccg ttcgctaaca gcctcatcga cgccggcacc
     2941 caactcggca tcgacaagtt cctgttggtg caatggcccc actcgcctcg gaggcaccca
     3001 aattcatcat cgcgatcatc ttcgccgccg gacgcctcat cctctgcggc atctacgccg
     3061 ccatcgcgct gcccgccctt atcgtcaacc gccagcacat cctgcccgcc ctcagcgcgc
     3121 cgttcgcccc gagcaccacg cgccacggcc ggcaccctca tccagcagca agcccgggcc
     3181 actgagggtg tctcgagtct ggccggtcga gctcgccgcg aagagagccg acgatgtctt
     3241 caaaatcggc ttggcccttc gtaattcagc gttctccagc ctcaagcgtt tcaactcagc
     3301 gggctcttcg gtcgtaattc cgggacgcgc cgccatcaac ctgggcctgg cgcatggaag
     3361 tagcccggcg cgcactactt cctcggagcg gcactcgccc gatggtgaca atcaatgcgt
     3421 tcctgggacg taatgctctg tcgcattcgc gatgtcgatc attccgtccg gatcgaccga
     3481 aacaccaacc cgtactcctc gagtgcgact acggcgctgt cggtcgccgc gacggcgtcc
     3541 acccggatcg ctgcggcagg actacggctc tttaccaacg cgtcgatgtc gcgccaaatg
     3601 gtttggctga gttgtcgtcc agaccggtga tcgaccagca gggccgcact gacgagcccg
     3661 tcggtttcgg tcagccaggg caccgctgtg tcttcatatc cggcgatcgc ctcgtcgagt
     3721 gcggccggat tcgtctgata gacggtgtgg cgcacgccgg ctccgccctt ttctcgcccc
     3781 acccgagtca cgctcgccct ccgaaagcgt tcgaccgagg cggtaccgag cgcaagacgg
     3841 atcgcttcgt cgcggacacc ggcctctacg cgttcgcttt ctcgcatggc gtcgtgtgag
     3901 acccagaacg actcgaggac cgcgacggcc aggtcgggag atacctgcag cgacattcct
     3961 ttcgcgccag gttctgactc aaggcgcgat cggccatcag aagcgaggta tgcgacggcg
     4021 tcgtcaatcc gttcagggtc ggcggttatc agcgtgtacc gaacatgcat ctgtggctcc
     4081 ttcactcgaa cgaccgcggg gactgtgccg ccatgaggac ggcaagcgtg cgttcggccg
     4141 cctgcttcga ggacgccgga ttctggccgg tgacaaggcg cccgtccacc accacgtgcg
     4201 aactccaggg ctgcccctga tcgaagtccg ctccctcctc gcgcagtcgg gtctccagta
     4261 gccacttggc acggtcggcc agacccgctt ggcgctcttc ctcattcgtg aaggcggtca
     4321 gccgtcggcc ggcgaacagc caggtgccgt cctcgcggcg gcccgccagc agtccggccg
     4381 gcccgtggca taccgcagcg acgactcgtc ccgtatcgaa cagatcggcc agaattcggc
     4441 cgagaggtcg acagtccggc agatcttcca tcggcccgtg gccgcccgga atgaagacgg
     4501 cgtcgtagtt gcccaggttg tcggcggcgt gctccaccga cattggggcg gcgagctcgt
     4561 cctccagcga ttccaaatag cgacgaagct cggcggcgcg ttcttcgccg ccagcgcgat
     4621 cgggcgccag gctgacctga tcgacggtcg gccggacacc acccggagtg gcgatgtcga
     4681 cgtcgacctc ggcgtggcgg aaggtgcggt ggggttcgac gaattcttcg gcccagaatc
     4741 cagtgggatg gcgggtgccg tcagacaacg tccagtgatc gctgccggag agcacgatca
     4801 gtattcgagc catggagtac ctccttgatg atccgaccgt cttcgtcgac atgctgccgc
     4861 tgacgctgcc tgcggtcttg gacacacagg tgcgaaaact gtcggcctcg ctagacctga
     4921 gggccttttg cagcggtctc gatctgccag aacaggtggt ccatcgactc ttactaacca
     4981 caggttggtg cgccgcaacg cagaagcggg cattgtccgg gaaagccgcc gttctgtgga
     5041 acagacagtt ggagatgatc cgatgaatgc ccacgagttt tatcgcgacg tcgccgagcg
     5101 cgccatgctg tcgaaggggg aggcagccga tctgacccgc acggtgctcg aggccttggc
     5161 gatgcgagtc agcgccggag aggtacggca cctgatccga cagctccccg aggagcttgc
     5221 cgactcagtg cgatggaaaa ccaaaggtcc ggagcgattc gacctggatg acctgattat
     5281 gcgcgtcagc agccgcactg ggctgaccaa gaccgagact ttgaccggtg tggaggcggt
     5341 gctgctgacc ttgcgggagg cggttgactg gaaggagttc cacgacttca tgtcgcagct
     5401 ccctgtggag ttcacccggc tgctcccgtc gcctaatccg ccacgggcgg tataaccggc
     5461 tggatgtgtt catggccggc gatgacgcgc aggccaaagc gagcgtgtct gcgttcatca
     5521 agagcctcgg actgcgcctg caggacaccg gcgacctgag catggcgcac gggctggagg
     5581 gcgcgggcct gctgtcagta ggcgtgggcc gc
//
LOCUS       DASRJA010000020        24592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1954594,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000020 DASRJA010000000
VERSION     DASRJA010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 24592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24592
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954594"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2414)
                     /locus_tag="VFC01_00820"
     CDS             complement(<1..2414)
                     /locus_tag="VFC01_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HZN78218.1"
                     /translation="MTVGFNFLEAPELPAPQVSEEQAEEILATHYGLQARATSLGSNQ
                     DKNFVVTGADGEIVGVLKIANPAFNATEIEAQDVAADRIAEAEPTLRMAVPLPNLAGE
                     KCTAIAGLVDGTAYVRLLRFLSGGTLIERGYLSPTAIAGLGEVAARVSRALEGFRHPG
                     LDRVLQWDLRYGTAVVAELISYVADPLHRARLEAAARDAWGRITPLADALPRQAVHLD
                     LTDANVVVSPSAGGSVPDGVIDFGDLSDSWAVSELAITASSVLGHPGSEPISILPAVR
                     AFHAIRPLSPAEVDALWPLLVLRTAVLIVSGAQQATLDPDNPYLTDQSGGEWRMFEQA
                     TSVPIDVMTAAIRADLGLATQPEPVPGTLIDVDPSSVVTLDLATTSDVYDFAFEADGS
                     LRAGVEDELARAAVQNGAGLVVTQYGQPRLSRTRKLSQDSPDVVATGISIWPASSVGL
                     VAPWDGEIHGSTLRGADYELTLTGVDMTSGSVRAGDPLADAPAGAWVEVGVRPVGAPP
                     APRFVPSELAPGWLAHTRDPRPLLGLPAAEAVSADDLLSRRDASFAPVQEFYYRKPPQ
                     IERGRRHYLMSTAGRTYLDMVNNVTVLGHAHPRIADTAARQLRKLNTNSRFNYAAVVE
                     FSERLAALLPDPLDTVFLVNSGSEASDLAIRLATAATGRRDVVAVREAYHGWTYGTDA
                     VSTSTADNPNALATRPDWVHAVESPNSFRGKYRGAEAVKYGGEAVQQISRLVADGRPP
                     AAFICESVYGNAGGMALPEGYLQQVYTAVRGFGGLAISDEVQVGYGRLGEWFWGFMQQ
                     DAVPDIV"
     gene            complement(2411..3265)
                     /locus_tag="VFC01_00825"
     CDS             complement(2411..3265)
                     /locus_tag="VFC01_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN78219.1"
                     /translation="MSTTRSEPQTVTFRGSEDLVLVADEWNSTAATGPSVLLLHGGGQ
                     NRFSWKNTGQILADEGFHVIALDSRGHGDSDRSADATYTVDALCADTLAVLEQIGRPV
                     ILIGASMGGMTGILAADAAGPEKVTKLVLVDVVPRYEKDGSARIRDFMFSHVHGFETL
                     EQAADAVAEYLPHRTKPRSPEGLKKNLRRRDGRWYWHWDPKFLTAPSDDAFVREEKLE
                     RAAIDLKIPILLIRGKLSDVVSPEGVKDFLKKVPHAEFVELSDAGHTAAGDDNDAFSE
                     AVVQFVSR"
     gene            3367..3981
                     /locus_tag="VFC01_00830"
     CDS             3367..3981
                     /locus_tag="VFC01_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78220.1"
                     /translation="MQAESASDADRGCIIDAAYGCLSEPHSGPIPVAAILQRAGVSTR
                     AFYRHFESKDELFLAMLRQESDVLAGRLDRIVDEAGTPVDQLKAWISQMFGWMHDAEM
                     RMHLTVIDSDEVRAAKGYRETRERLHADRERSLIEILRRGRDDGSFPQADPDHDAVAI
                     SAVISREMADQRPGDEQRLRRSLAWVLEFALRALGAEGASADKR"
     gene            4035..4508
                     /locus_tag="VFC01_00835"
     CDS             4035..4508
                     /locus_tag="VFC01_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN78221.1"
                     /translation="MPSITPCLWFDNNLEEAAQFYTSIFPNSTIEKFERYTDAGPGTP
                     GDVVWGAFVLDGTRFMGINGGPAFQFSEAVSFMVECKNQDEVDYYWERLVDGGEESQC
                     GWLTDRFGVSWQVTPTRLLELESDPDPARATAATKAMHGMRKIVIAELEDAVRDA"
     gene            4841..5608
                     /locus_tag="VFC01_00840"
     CDS             4841..5608
                     /locus_tag="VFC01_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN78222.1"
                     /translation="MVASNLVAKPVRGVGGFYAMALDTFVAMVKPPFAWREFISQSWF
                     VARVSIVPTLMLTIPYTVLLTFTFNILLTEFGAADFSGTGAALGTVRQIGPIVTVLVV
                     AGAGATAMCADLGARTIREELDALRVMGVNPIQALVVPRVLAATLVSLALAATVILVG
                     LAGAYLFCVYIQHVSPGAFAAGLTLIVGAADVTIALLKAALFGLSAGMIACYKGISVG
                     GGPAGVGNAVNETVVFTFMALFAINVVATAVAVKVTM"
     gene            5605..6459
                     /locus_tag="VFC01_00845"
     CDS             5605..6459
                     /locus_tag="VFC01_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN78223.1"
                     /translation="MTIARPHSQFPWLKARFRSVADPWNRLGRQTKFYGRTVRSIHYA
                     FTHYRVELIRLIAQMGLGAGALIIIGGTVAIVGFLTVTTGALVAVQGYTDFSEIGVEA
                     LTGFASAFFNVRLIAPATTAVALSATIGAGATAQLGAMRINEEIDALEVMGIRSVAYL
                     ASTRVVAGLLVVIPLYCVGVIAAFWAAKFGTTVLYGQSTGVYDHYFRTFLNPTDLMWS
                     LIQSVVLAAVIMLVHTYYGYTARGGPAGVGEAVGRAVRTSLILAAFVLVMMSLAVYGQ
                     SGNFNLAG"
     gene            6463..7914
                     /locus_tag="VFC01_00850"
     CDS             6463..7914
                     /locus_tag="VFC01_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN78224.1"
                     /translation="MDSDDSGLHPGWYTLILVVVFAVLIWLSYALFAGTLKDDMPVTL
                     TSDRAGLVMETNAKVKLRGVQVGRVSGITGGKEPVVLKLEIDRDQIKYIPANVEAQIR
                     ATTVFGAKFVDLVYPDDPSPQRLAENQVLKSRNVSTEVNTVFQNVVSVLNQIDTAKLN
                     STLAALAEGVRGQGEKIGQATTDANQVLLQLNPRSDTIRADWQALKGFNDAYSGAAQD
                     ILTVLNAASTTSQTVTSHTKQLDALLLATIGLSNSGISLLAPNQANLIKAINVLEPTT
                     NLLYKYNPEYTCLLVGAKTLLDTGGYEAPGGNGRTLVLDTGLSLGDDPYHYPTNLPVI
                     GAKGGPGGKPGCGSLPDVAKNWPVRNLVTNTGFGTGVDWRPNPGIGFPAWANYLPVTR
                     AVPEPPSIRNLFGGPAPGPIPYPGAPAYGADLYAPDGTPLWPGLPPAPPPMAPRDPGP
                     TPGSEPFVVPAPAQMQPTPLPPIPLPREAAPSP"
     gene            7957..8988
                     /locus_tag="VFC01_00855"
     CDS             7957..8988
                     /locus_tag="VFC01_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HZN78225.1"
                     /translation="MRENLRATAVRLAVFLAVCLLGVFGLYAVFGQLRFGEKAHTYQA
                     AFINVTGLEKNDFVRIAGVEVGQVKKVAIQPDTTALVEFTADDSVVLTEGSRAVIRYD
                     DLIGGRYLALEEGAGGTKKLNPGDTIPLARTSPALDLDALIGGFRPLFRALDPDQVNA
                     LSGQLITALEGQGGTINSFLAQTAALTTTLADRDQLIGQVIVNLNTVLGSLGDQSDQF
                     AKAVDALSELVKGLESRKQDISNGLAYTNAAAGSITDLLSQARPPFAKTIHETDRAAG
                     IVVADHDYFDNLLNTLPDAYQALARQGIYGDFFSFYLCDIVLKLNGKGGQPVYVKVAG
                     QSTGRCAPR"
     gene            8985..10319
                     /locus_tag="VFC01_00860"
     CDS             8985..10319
                     /locus_tag="VFC01_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN78226.1"
                     /translation="MKPFSDRNLFIIGAIGLGITVAIILGAINYDKLPFLNQGKEYSA
                     YFAEAGGLMDDAAVQVSGFQVGKVESIELDGPRVLIKFTVDKNIRLGNRTEAAIKTKG
                     LLGNKILEVTSRGDGRQEGTIPLDRTTSPYQLPDALGELATTISGLNTNQLSDSLRVL
                     ATTFADTPPELKIAVEGVARFSDTLNERDAQLRGLLTNADKATTVLAERSNQVVSLIG
                     NTNSLLVELQSQSAALDQISGNISALSQQLQGFIAENRDTMRPALDKLNGVLTMLDNR
                     KERLQRSIKLLTQYSMSLGESVASGPFFKTYVANLLPGQFVQPFIDAAFSDLGLDPNT
                     LLPSERTDPQIGQPGTPALPVPYPRTGQGGEPHLTLPDAITGNPGDQGCGPPGLPLPG
                     PTGCYPYREPLPAPPPGGPPPGPPALAPPGLDSTPQPTPSPVMVPAPGEAGP"
     gene            10316..11641
                     /locus_tag="VFC01_00865"
     CDS             10316..11641
                     /locus_tag="VFC01_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN78227.1"
                     /translation="MNRIRKSWVAVALVVLLVAGVAVLFRTSDTINRTNVVAYFENSN
                     GVYVGDDVRILGVNVGRITEIQPQPERVKVSFWYDSKYKVPADAKAAILSPTLVTARA
                     IQLTPVYTTGPVMPDDAVIPQERTAVPVEWDQVRAQLEKLAHTLQPTEPGGVAPLGSL
                     INTTADNLRGQGASIRDTVIKLSQAFSALGDHSTDIFSTIKNLAILVSALQDSTNLMR
                     QLNQNLASVTGLLADNPNEVGDAVRDLNAAVGEVQTFVADNREALGTTSDKLAGVTQA
                     LTDSLDDVKQFLHVAPNTLQNYVNIWQPAQGAISSVPMINNFANPISFLCGAIQAASR
                     LGAEESAKLCVQYLAPIIKNRQYNFLPVAQNVFVGATTRPNELTYSEDWMRPDYIPPQ
                     PTPGAGPPAATSPEAAPPPPGPPLPAEAPVATNPADGLTGMMVPPGAGP"
     gene            11638..12795
                     /locus_tag="VFC01_00870"
     CDS             11638..12795
                     /locus_tag="VFC01_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HZN78228.1"
                     /translation="MMRAKWIRIAVSLAVLALAVGGCADWQGLNSLPLPGVQGTGPGG
                     FTVQAQMPDVDNIEQNSRVRVGDVNVGTVTKIERQGWHALVTMKLNGDVQLPANATAK
                     LGQTSLLGSLHIELAPPTDVAPQGTLRDGSLIPLESSGKYPSTEQTLAAIALLLNGGG
                     IGNIQDITDALSIAFTGRENDLRSLIEQLDKAIGYLNDQKHDIIAASESLNNLIGQFA
                     EQKPVVDKALKTIPDALVVLKDQRDNLSEALAQLGKFSALAADSVNQTKEALVQELKD
                     LGPVLQSLADAGPALTRALSFLPTFPFPKETLTNWMRGDYANLTLILDLTLSRIDTGF
                     FTGSRWECDLTWLEIQWGRTIGQFPSPCTGSAPPNGAGNPLIAPYHWDQGP"
     gene            12797..14299
                     /locus_tag="VFC01_00875"
     CDS             12797..14299
                     /locus_tag="VFC01_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZN78229.1"
                     /translation="MILTRRILIQMAIFAVVATTALAVMLFGYMRVQDMAGIGQYKVT
                     VQLPETGGLYPRGNVTYRGAQVGTVKSVHLTDSGVAAELSLDSNVKIPADVEAEVHSV
                     SSVGEQFVQLLPRSGKGPTLKDGDVIPADRTTVPTDINKVLADTTRGLQAIPQENLKT
                     VIDEAYVAVGGLGPELRRLVNGSTNLAIDARKNLDPLTTLIDQSKLVLDTQTETAGSI
                     QAWASNLATITTQLQNQDAAVAGVLDKGPGAADEVRALFDRLQPTLPIVLANLVSIGE
                     VAVTYQPSLEQLLVLLPQGTAVTQAVGVHKRNTKQDYEGDALVFNLNLLIPLLPAPIP
                     LPPQQIPPPCTTGFLPAQQQRVPTFEDYPDRPPGDVYCRVPQDAPFNVRGARNLPCET
                     VPGKRAPTVKMCESNEYYVPLNDGYNWKGDPNATLSGQAVPQLPPGSPPAQAAPPPGP
                     SPPPIAAAEYDPATGTYVGPDGRVYTQSNLARTATEEQTWQTMLLPPTGN"
     gene            14263..14982
                     /locus_tag="VFC01_00880"
     CDS             14263..14982
                     /locus_tag="VFC01_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mce protein"
                     /protein_id="HZN78230.1"
                     /translation="MADDAAAPDGELSKSTATEETSSATEPESGGSDVSDAADSDAAV
                     TDEVTDDADSDEEAAHSEEEADSEETTEAAPAKPPMSHVKLAMIAGLVLVVALGGLTG
                     WLGYRAYQSHQADEQRKLFLSVGRQGALNLTTIDFQHADADVQRILDSATGTFYDEFQ
                     KRAQPFVEVVKQAQSKSEGTIAEAGLESESDKGAQVLVAVTVKTSNAGAPDQAPRAWR
                     MRISVQKVGDDAKVSNVDFVP"
     gene            14979..15632
                     /locus_tag="VFC01_00885"
     CDS             14979..15632
                     /locus_tag="VFC01_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78231.1"
                     /translation="MSNHSQDQTAEEATLTSDTETEQIEDAVSDETEKADEPTETADE
                     ATESAEPAKPKQKIQWPRVFAYGVLPALALLLAMGAGYLKWTDNSVRDDQTARIQAMQ
                     AAKDSTIAMLSYKPDTVEQQLTAARDLLTSDFRDQYTSLTNDVVIPGAKQKQISAVAT
                     VPAMASVSANPRHAVVLVFVNQTVIVGQDAPTDSASSVRVTLDKVGDRWLIAKFDPV"
     gene            15634..16536
                     /locus_tag="VFC01_00890"
     CDS             15634..16536
                     /locus_tag="VFC01_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN78232.1"
                     /translation="MTEPQWLDVAAPAVQLRALTWGPADAPIALCLHGFPDTAYGWRK
                     VAPALVDAGWRVVAPFMRGYAPSSLPSDGSYHVGAYMDDALRVLKAAGPTGRDVFIGH
                     DWGAIAGAGLAAMPDSPFTKAVIMSVPLSAAFRPFGRVPQAGRLAAKLPRQLLNSWYI
                     LYFQLPWLPDRSASWVIPRLWRDWSPGYRADDHLRYVDASIGAPDRWRAALGYYRATI
                     RGSRPPAQYAELNQHWLDAPRLPTLYLHGTDDGCSEDYTPWIERVLPDESEIALIKNG
                     GHFLQLDQPDVVGRHIVDFVGQAR"
     gene            complement(16533..17336)
                     /locus_tag="VFC01_00895"
     CDS             complement(16533..17336)
                     /locus_tag="VFC01_00895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN78233.1"
                     /translation="MLPPISGDGVVVVTGASSGIGEEIAREFARRGYRLVLVARRADR
                     LRALAEEFNGRAHVLPADLSNRDERATLPDQVAALGVVAEVLINNAGLSTLGPVADSD
                     PEAELNLIEVDVAAVVDLCSRFVPDMVKRGRGAVLNVASVGAFGPVPGQASYGAAKAF
                     VLSYTEAMREELRGTGVTVSALCPGPVRTTFGEAAGIPQEEAEKSLPKFLWEEPDAVA
                     KAAVDGLASGKRVIIPGAANRIAAVINHHLPRRLVTRLVARNHPGLKKG"
     gene            17359..18609
                     /locus_tag="VFC01_00900"
     CDS             17359..18609
                     /locus_tag="VFC01_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HZN78234.1"
                     /translation="MMVRRLSAVDAQTYWMSAKIPNDQFLLYGFAGVPDVDHAVDVIR
                     QRSRDCPELRLRVRDRGFFTYPEWVAGEVRPGQFVVHDVVDSSWSECLAGVARLAEDQ
                     LDPQAMTWRLHVFAGVDVPGAGRGSVLVLQAAHVFGDGIRSSALAAWLFGRTEPVPAV
                     RVPPLQTLRLPWRSFRAARTHRQLVRDVDAGLVPPQADSRPALRSNARPDGVRSIRTV
                     IRSRAQLPGPTITVGVLAAVSTALAAHLCELGDDPSALGAEVPMAKAGVRQAHNHFGN
                     VGVGLYPDLGSDERAARIAEDLADRRRRAAHPGMRAASRAFAAVPAPLLRWGVKQFDP
                     TVRSPVVTGNTVVSSVNRGPADLRFGDAPVVLTAGYPGLSPMMGLTHGVHGIGDTIAI
                     SVHAAESAIGDVDAYVERLEAALG"
     gene            complement(18606..19214)
                     /locus_tag="VFC01_00905"
     CDS             complement(18606..19214)
                     /locus_tag="VFC01_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZN78235.1"
                     /translation="MKIAIIGAGNVGRALGRAWRTDHDITYGVRSPNDPKYADLGAPA
                     TTNDAAVAGSDVVVLCTPWQGAEAALKACGDLTGKVLIDCVNPLTPDFSALEIGHTTS
                     GAEQVAAWAPGARVCKAMNQIGAPMMDHPQLPSAPVMFVCGDDDAAKQVTADLVTQLG
                     FEAVDAGDLTLARLLEPFALVWIHLALRRGFGTNWGFGLLRG"
     gene            19290..19613
                     /locus_tag="VFC01_00910"
     CDS             19290..19613
                     /locus_tag="VFC01_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN78236.1"
                     /translation="MDDQFGCPVEATLAVVGGKWKAVLIFHMMHTGPHRFAELKRKTT
                     GISDRVLTRQLRELETDGIVRREVFAEVPPRVEYSLTEYGESLRPVTEAMCQWGKRHM
                     STGAA"
     gene            complement(19685..20290)
                     /locus_tag="VFC01_00915"
     CDS             complement(19685..20290)
                     /locus_tag="VFC01_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78237.1"
                     /translation="MDLQVQKLTPKGQATRQRIIEGAAAEIREHGVAVATLDDIRART
                     QTSKSQLFHYFPDGKDQLLLAVAEHEAQMVLDDQQPYLGELTSWAAWQRWRDAVVDRY
                     RRQGQHCPLAVLMSEIGRSTPGAQAVTSTLLRKWTDEIAAGVRHMQADGKVAARLDAD
                     RIAAALLAAVQGGVGVMLATGDLSYLEAALDVGIESLRASD"
     gene            20315..21079
                     /locus_tag="VFC01_00920"
     CDS             20315..21079
                     /locus_tag="VFC01_00920"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZN78238.1"
                     /translation="MSSNTARLAGRTALVTGSTGGLGVAIASALAAEGASVIVSGRDK
                     ARGDAVVADIRSAGGAAEFVVADLAAGDQEVRRLAEQATAAAGGRIDILVNNAAMLLM
                     PTPTAEVSDEVLRDAFAVNVFAPFLLTGQLAPAMVQRGEGAIVNIGSITGLRGSAGSA
                     IYSANKAALHSFTQSWADEYGPSGVRVNAVAPGPIATERQEQFADHVAPMVARIPSRR
                     MSTPAEVAASVVFLASDDAANIHGTVLGVDGGWAAV"
     gene            21102..21632
                     /locus_tag="VFC01_00925"
     CDS             21102..21632
                     /locus_tag="VFC01_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZN78239.1"
                     /translation="MTDHTAIDTGLLILRVVLGLTMAAHGYNKFFGKGGLAGTAGWFD
                     SMGMKPGMFHARIAATTEMSAGLGLAAGLLTPIPAAGFVALMFVAAWTVHRPNGFFIV
                     KEGWEYNLVLAVSAVAIATIGPGKFSLDYALFRTTGLYDFLHGWWGLLISAGLGLAGG
                     IGQLVIFWRPPAKADA"
     gene            complement(21629..22333)
                     /locus_tag="VFC01_00930"
     CDS             complement(21629..22333)
                     /locus_tag="VFC01_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN78240.1"
                     /translation="MTTFALVHGAWHGAWCWEQLTPLLRQAGHDVVTMDLPNEDGTAT
                     FDTYADVVCAALDSRGDDVVLVGHSYNGNTIPLVAARRSVRHLVYLCAMVPDIGRSVF
                     DQMSDELEMLNPVAYEQGLSVPDEQLRQVWTDLDLACAMFYGDCDEATTRAALERLRP
                     QSAYPAILPSSLAEFPAVKTTYVACSDDQILRPAWSRQTARERLGAELIELPGDHSPF
                     YSRPSVLADVLLGLAS"
     gene            22389..24137
                     /locus_tag="VFC01_00935"
     CDS             22389..24137
                     /locus_tag="VFC01_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HZN78241.1"
                     /translation="MGFLKQDAPVVDYEEWSKGSRAERIVPMAKHWAEVGFGTPVVLH
                     LFYVVKILLYILGGWLFALATEGIDGFTNVASWYAEPIVFEKVVLYTMLFEVVGLGCG
                     FGPLNNRFFPPMGSVLYWLRPNTIRLPPWPNRIPLTRGTTRTPVDAALYGALLVMLLV
                     ALFSDGTGPIPALGTTVGVLPMWQIWTIVGLLAVLGLRDKVIFLAARGEVYASFTVAF
                     LFAGYGVDMIIAAKLVCLVIWLGAATSKLNKHFPFVISTMMSNNPVFRPRFIKRKFFE
                     HFPDDLRPGRLSRWLAHFSTAIEGLVPLVLFFSHGGWPTAIAAFVMLCFHFGILSSIP
                     MGVPLEWNVFMMFSVLALFVGHAEVGPGDMTTPLPVLLFGVVAGTVVIGNLFPRKVSF
                     LPGMRYYAGNWDTGLWCVKPSAAEKIEANVVSIASMPQAQMERYYGSPETAQMYLYMG
                     YAFRSFNTHGRAMFTLAHRAMAGQNEDDYVLTDGERICSTAIGWNFGDGHMHNEQLIE
                     ALQERCHFEPGEIRILLLDAQPIHKQRQEYRLVDSATGEFERGYVKVADMVTRQPWDD
                     TVPVHVTWEKDSADAT"
     gene            24142..24507
                     /locus_tag="VFC01_00940"
     CDS             24142..24507
                     /locus_tag="VFC01_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78242.1"
                     /translation="MSVWFDETGQLMSDLGPVDRNCVVRVISGHCPDRHQCVLLYRAP
                     GPRLLYGSELMSELDDERGLYLETHAKHLEDDLISITVDHVGVDGHADGWRYRLLPMR
                     WKTEDGLVDTPTRLAVWPD"
BASE COUNT         4302 a         8055 c         8015 g         4220 t
ORIGIN      
        1 acgatgtcag gcaccgcgtc ctgctgcatg aaaccccaga accattcgcc gagacggcca
       61 tatccgacct gcacctcgtc ggagatcgcc agcccgccga acccccgcac cgccgtatac
      121 acctgctgta ggtagccttc cggcagcgcc atgccccccg cattgccgta cacgctttcg
      181 cagatgaacg ccgcgggcgg tcggccgtca gccaccagcc gcgagatctg ctgcactgct
      241 tcgcccccgt acttgaccgc ctcggcgcca cggtacttgc cgcggaaact gttcggcgat
      301 tcaaccgcgt gcacccaatc cgggcgggtg gcaagcgcat tggggttatc ggctgtcgat
      361 gtggacaccg catccgtgcc gtatgtccag ccgtgatacg cctcccgtac tgcgacgaca
      421 tcacgccgac ccgtcgcggc cgtcgcgagc cgaatcgcca gatcgcttgc ctccgagccg
      481 gagttgacca gaaagacggt gtccagcgga tcaggcagca gcgccgccag tcgctcgctg
      541 aactcgacca ccgccgcgta gttgaaccgc gaattcgtgt tcagcttgcg cagctgcctg
      601 gctgcggtgt cggcgatccg cggatgggcg tggccaagca cggtcacgtt gttgaccatg
      661 tcgagatacg tgcggccggc ggtcgacatc aagtaatgcc ggcgccctcg ctcgatctgc
      721 ggtggcttgc gatagtaaaa ctcttgtacg ggagcaaaac tcgcatcccg ccgcgacaac
      781 agatcgtctg cagacacagc ctccgccgca ggcagcccca gcagcggtcg cggatcgcgg
      841 gtgtgcgcca gccagcccgg cgcgagctcg gacgggacaa accgaggtgc cggcggcgcc
      901 ccgaccggcc gaacgcccac ctccacccac gcgccggcgg gcgcatcggc aagcgggtcg
      961 cccgctcgca cgctgcccga cgtcatgtcc acaccggtca atgtcagctc gtagtcggct
     1021 cctcgcagcg tcgacccgtg gatctcaccg tcccacggcg ccacgagccc tacagaagac
     1081 gccggccaga tgctgatgcc ggtcgcgacg acgtccgggc tgtcctggct gagcttgcgg
     1141 gtccggctca gccgtggctg accgtactgc gtgacgacga gcccggcgcc gttttgcact
     1201 gccgccctgg ctaattcgtc ctcgacgccc gccctcagtg atccgtccgc ctcgaatgcg
     1261 aagtcgtaaa cgtccgacgt cgtggccaag tcgagtgtca ccaccgacga tgggtccacg
     1321 tcgatgaggg tgcccggtac aggctccggc tgtgtggcca gtccgaggtc ggcccgaatc
     1381 gccgcggtca tcacatcaat cggcaccgag gtcgcctgtt cgaacatgcg ccactcgccg
     1441 ccggattgat cggtcaaata ggggttgtcc gggtcgaggg tggcctgctg cgcgccactg
     1501 acgatgagta ccgcggtgcg cagcaccagc agcggccaca acgcgtccac ctctgccggg
     1561 gacagcggtc ggatcgcgtg aaacgcccgc accgcaggca ggatcgagat cggttcgcta
     1621 ccagggtggc ccagcaccga cgacgccgtg atcgccaatt cggataccgc ccagctgtcg
     1681 gacaggtcgc cgaagtcgat gacgccgtcg gggacagagc ccccggccga tggtgagacc
     1741 acgacgttcg cgtcggtgag atccagatgc acggcctgcc gcggcagcgc atcggcgagt
     1801 ggcgtgattc gtccccaggc gtcgcgcgcc gccgcctcca gacgcgcgcg atgcagcggg
     1861 tcggccacat acgaaatcag ttccgccact acggcggtgc cgtaccgcag atcccattgc
     1921 agcacccgat caaggccggg atgacggaaa ccctccagcg cccggctgac ccgggcggcc
     1981 acctctccca gtcccgcgat cgcggttggc gacaggtaac cccgttcaat cagcgtgccg
     2041 ccggacaaga agcgcagcag ccgaacatag gcggtgccat ccaccaatcc ggcgatggcc
     2101 gtgcactttt cgcctgcaag gttcggcagt ggcaccgcca tgcgcagcgt cggctcggcc
     2161 tcagcgatac ggtccgccgc cacgtcctgc gcctcgatct ccgttgcatt gaacgcggga
     2221 ttggcgatct tcaacacccc gacgatttcg ccgtcggcac cagtgacaac gaaattcttg
     2281 tcctggttgc tgcccagcga cgtcgcgcgg gcctgcagcc cgtagtgcgt ggccagaatt
     2341 tcctcggcct gttcctcact gacttgaggc gccggcagct ccggtgcctc aaggaaattg
     2401 aagccgacgg tcaccggctg acgaattgga cgacggcttc ggagaacgcg tcgttgtcgt
     2461 cgcccgcagc ggtgtgtccc gcatcggaca gctcgacgaa ctcggcgtgt ggcaccttct
     2521 tgaggaagtc cttcacgccc tcggggctga ccacatcgga cagcttgccc cgaatcagca
     2581 ggatcgggat cttcagatcg atcgccgcgc gttcgagctt ctcctcgcgc acgaacgcat
     2641 cgtcggatgg cgccgtcaga aacttcggat cccaatgcca gtaccaccgg ccgtcacgtc
     2701 ggcgcaggtt cttcttcagg ccctccgggc tgcgcggctt ggtccggtgc ggcagatact
     2761 cggcgaccgc gtctgcggcc tgttccagcg tctcgaagcc atggacatgg ctgaacatga
     2821 agtcgcgaat ccgggcgctg ccgtccttct cgtaccgcgg caccacgtcg acgagcacaa
     2881 gtttggtgac cttctccggg cccgcggcgt cggcggcgag gatgccggtc ataccgccca
     2941 tgctcgcgcc gatcaagatc accggccgcc cgatctgctc cagcacagcc agcgtgtcgg
     3001 cgcacagtgc atcgaccgtg taggtggcat cggcggagcg gtcgctgtcg ccgtggccgc
     3061 ggctgtccag cgcgatgacg tggaaaccct cgtcggccag gatctggccg gtgttcttcc
     3121 aggaaaagcg gttctgcccc ccgccgtgga ggaggaggac ggacggccca gtggcggctg
     3181 tggagttcca ttcgtcggca accaggacga ggtcctcgga cccgcgaaac gtaacggtct
     3241 gcggttcgct gcgggtagtg ctcatcgacg tcctctcggc cggccccgaa cccagacgtt
     3301 acccagagga caatttgcgc ccatcttcgg cccggctggt gcgacgcatc cctagaattt
     3361 gcgatcgtgc aggcagaatc cgctagcgac gccgaccgcg gctgcatcat cgacgccgcc
     3421 tacggctgcc tgtcggagcc gcacagcggt ccgattccgg ttgccgcgat cctgcaacgg
     3481 gccggggtgt cgactcgggc cttctaccgt cacttcgagt ccaaggatga gttgttcttg
     3541 gcgatgctgc ggcaggaaag cgacgtgctc gcaggtcggc tcgaccgcat cgtcgacgaa
     3601 gcgggcactc ccgtcgacca gctgaaggca tggatcagcc agatgttcgg ctggatgcac
     3661 gacgcagaga tgcgcatgca cctgacggtg atcgactccg atgaggtgcg ggccgccaag
     3721 ggctatcggg agacccgcga gcgcctgcac gccgatcgcg agcgctcgct gatcgagatc
     3781 cttcggcgcg gccgggacga cggctcgttt ccgcaggccg acccggacca cgacgcggtc
     3841 gccatcagcg ccgtcatcag ccgcgagatg gccgaccaga ggccggggga tgagcagcgg
     3901 ctgcgccggt cattggcctg ggtcctggag ttcgcgctgc gtgcgctcgg tgcagaaggt
     3961 gcaagcgccg acaagcgatg aatcacgggc gcctggccgg tctaacacgg gagagccgac
     4021 ttgaaggagt agcgatgccg tcgatcaccc catgcctctg gttcgacaac aacctcgagg
     4081 aagccgcgca gttctacacg tcgatctttc cgaactcgac gatcgagaag ttcgagcgct
     4141 acaccgacgc agggccgggc acgcccggcg atgtcgtgtg gggcgcgttc gtgctcgacg
     4201 gcacccggtt catgggcatc aacggcggac cagcgtttca gttcagcgag gcggtgtcgt
     4261 tcatggtcga gtgcaagaac caggacgagg tcgactacta ctgggaacgg cttgtcgacg
     4321 ggggcgaaga gtcgcaatgc ggctggctca cggaccgatt cggggtgagc tggcaggtga
     4381 ccccgacccg gctgctcgag ctggaaagtg accccgaccc ggcccgtgca actgcggcga
     4441 ccaaggcgat gcacgggatg cgcaagatcg tgattgccga gctggaggac gccgtccgcg
     4501 acgcgtagta gtagctcacc ccggggtggg gtgtccgatc cgagcacaag ttaatgggca
     4561 ttccgcccgt tatggaatgc gatactggaa gcgcggcgtt tgctgcgacg gttcgctggc
     4621 gcccatcgta gggcatgcat tgacagtgat gcgatcggtt atccggcagc aaaagtggcg
     4681 gtcgtcacca cagtgggact tgtgttgccc ggccagaaat cggtgatgta cggttctgcg
     4741 gcggccactc gtgtaacact cacaacttgc gcgtacgggt tgttcatcgg agggatcggc
     4801 agcctatggc ggtgaggaga acgcgaggga gggcccggta atggtcgctt ccaacctcgt
     4861 tgcgaagccg gtacgcggtg tcggcggctt ctatgcaatg gcgctcgaca ccttcgtggc
     4921 gatggtcaag ccgccgtttg cgtggcgcga attcatttcg cagtcttggt ttgtcgcgcg
     4981 agtttcgatc gtgccgacgt tgatgctgac gattccgtac accgtcttgc tgacgttcac
     5041 gttcaacatc ctgttgaccg agttcggcgc agcagacttc tccggtacgg gcgcggcctt
     5101 aggcaccgtg cgacagatcg gcccgatcgt caccgtgttg gtggtggccg gcgcgggggc
     5161 cactgcgatg tgcgccgacc tgggcgcgcg caccatccgc gaagagcttg acgcgctgcg
     5221 cgtgatgggc gtcaatccca ttcaggcgct tgtcgttccg cgggtgttgg ccgccacgct
     5281 ggtgtcgctg gcgctggcgg cgacggtcat tctcgtcggt ctcgctgggg cgtatctgtt
     5341 ctgcgtctac atccagcatg tgtcgcccgg tgccttcgcg gcgggcttga cgctgatagt
     5401 cggcgccgca gacgtcacga tcgcgctgtt gaaggcggca ttgttcggac tctccgcagg
     5461 catgatcgcc tgctacaagg gcatttccgt tggcggcggt cctgcgggtg tcggaaacgc
     5521 ggtcaacgaa accgtggtgt tcacgttcat ggcattattc gcgatcaacg tggtagcgac
     5581 cgccgtcgcc gtgaaggtga cgatgtgacg atcgcgcggc cccactccca gttcccgtgg
     5641 ctgaaagccc gattccgcag cgtggccgac ccctggaacc ggctcggccg acaaacaaag
     5701 ttctacggca ggacagtccg ctcgattcac tacgccttca cgcattaccg ggtcgaactg
     5761 atccggctga tcgctcagat gggcctcggc gcgggcgcgc tcatcatcat cggcggaacg
     5821 gtcgcgatcg tcggcttctt gaccgtcacc acgggcgcac tggttgccgt tcagggctac
     5881 accgacttct ccgaaatcgg tgtcgaagcc ctgaccggat tcgcctcggc attcttcaac
     5941 gtccgcctga tcgcacccgc aacgaccgcg gtcgcgctgt ccgcgacgat cggggcgggt
     6001 gccaccgccc aactcggtgc catgcgcatc aacgaggaga tcgacgcgct cgaggtgatg
     6061 ggaatccggt cggtcgccta cttggcttcc acgcgggtcg tcgcgggtct gttggtcgtc
     6121 attccgctgt attgcgttgg cgtgatcgcg gcgttctggg cggcgaagtt cggcaccacc
     6181 gtcctctacg gtcagtcaac cggcgtgtac gaccactact tccgaacgtt tttgaacccc
     6241 accgacctga tgtggtccct catccaatcc gtcgtgctcg cggcggtgat catgctggtg
     6301 catacctatt acgggtacac cgcgcgcggt gggccggccg gcgtcggtga ggcggtcggg
     6361 cgtgcggtgc gtacgtcgct gattctcgcc gcgttcgttc tcgtaatgat gtcgctggcc
     6421 gtctatggcc agtccggaaa cttcaacctg gccgggtagc agatggacag cgacgacagc
     6481 ggccttcacc cggggtggta cacgctcatc ctggtcgtgg tctttgctgt cctgatctgg
     6541 ttgagctacg cgctatttgc gggcaccttg aaggacgaca tgccggttac gttgacatcg
     6601 gaccgagcgg gcttggtgat ggaaaccaac gcgaaggtga agctgcgcgg cgtccaggtg
     6661 gggcgggtct ccggcatcac cggtgggaaa gaacccgtcg tcctgaagct cgaaatcgac
     6721 agggaccaaa tcaagtacat cccggcgaac gtcgaagcgc agatccgcgc cacgacggtg
     6781 ttcggggcca agttcgtcga tctggtgtat ccggatgatc cgagcccgca gcggctggcg
     6841 gagaatcagg tactgaagtc gcgcaacgtc agcaccgagg tcaacacggt gttccagaac
     6901 gtggtctccg tgctgaatca gatcgacacg gccaagctga acagcacgct ggccgcgctc
     6961 gccgaaggcg ttcgcggaca aggggagaag attggccagg cgaccacaga cgccaaccag
     7021 gtcctgctgc aactcaatcc gcgtagcgac acgattcggg ccgactggca ggcgctcaag
     7081 ggcttcaacg acgcgtatag cggtgcggcg caagacattc tgaccgtctt gaatgcggcg
     7141 agcacgacca gtcagaccgt caccagtcac accaaacagc tggatgccct gctgctggca
     7201 acaattggat tgtccaacag cgggatcagc ctgctggcac ccaatcaggc caatctgatc
     7261 aaggccatca atgtcctcga gcccacgacg aacctgctgt acaagtacaa cccggaatac
     7321 acctgtctgc tggtgggcgc gaaaaccctg ctggacaccg gaggttacga agcgcccggc
     7381 ggcaacgggc gaacactcgt gctcgacacc ggcctgtcgc tcggggacga cccgtaccat
     7441 tacccgacca acctgccggt catcggcgcc aagggcggac cgggcggcaa accagggtgc
     7501 ggctctctgc cggatgtcgc gaagaattgg ccggtgcgca atctcgtcac caacaccgga
     7561 ttcggcaccg gcgtggattg gcgaccgaac cccggcatcg gcttcccggc ctgggccaac
     7621 tacctgcccg tcacccgtgc ggtgcccgag ccgccgagca tccgcaacct cttcggcgga
     7681 cctgcgccgg gaccgatccc atatccaggc gcccctgcgt acggagcgga cctctacgcg
     7741 ccggacggca cgccgttgtg gccgggcctg ccaccggcgc ctccgccgat ggcgccacga
     7801 gacccgggtc cgacaccggg ttctgaaccc ttcgtcgtcc cggcgcccgc acagatgcag
     7861 ccaaccccgc taccgcctat cccactgccg cgggaggccg ccccatcgcc ctgatcgttc
     7921 gtcgccacaa ccatcaacca tgagaggtaa ccagtcatga gagagaactt gcgagccacc
     7981 gcggtgcgcc tcgctgtctt tctggctgtg tgcctgctcg gtgtgtttgg gctgtatgcg
     8041 gtgttcgggc agttgcgctt cggggagaag gctcacacct accaagccgc gttcatcaat
     8101 gtgaccggac tcgagaagaa cgacttcgtc cgcatcgcag gtgtcgaggt cggccaggtg
     8161 aaaaaggttg cgattcaacc cgatacgacg gcgctggtcg agttcaccgc cgacgattcg
     8221 gtggtgctga ccgagggcag cagggccgtc atccgttacg acgacctcat cggcggtcgc
     8281 tatctggcgc tggaggaggg tgcaggcggc accaagaagc tcaaccccgg cgacaccatt
     8341 ccgctggcgc gtacgtcacc ggcgcttgac ctcgatgcgc tgatcggcgg cttccggccg
     8401 ctgtttcggg cgctggatcc cgatcaggtc aacgcgctgt ccggccagtt gatcaccgcc
     8461 ctggagggcc aaggcgggac gatcaactcg ttcctggccc agactgcggc gctgacaacc
     8521 acgctcgcgg accgtgatca gctgatcggg caggtcatcg tcaacctgaa caccgtgctc
     8581 ggatcgctgg gtgaccaaag cgaccaattc gcaaaggccg tcgacgcgct ctccgaattg
     8641 gtcaaggggc ttgaatcgcg caagcaggac atcagcaacg gcttggccta cacaaacgcc
     8701 gccgcgggaa gcatcaccga cctgttgtca caggcccgtc caccctttgc gaagacgatc
     8761 cacgagaccg accgtgcagc ggggatcgtg gtggccgatc acgattattt cgacaacctc
     8821 ctcaacaccc tgccagacgc gtatcaagcg cttgcgcggc aaggcattta cggtgacttc
     8881 ttcagcttct atctgtgcga catcgtgctg aagctgaacg gcaagggggg ccagccggtg
     8941 tacgtcaagg tcgcgggcca gagcaccggg aggtgcgcgc cgaggtgaag cctttctctg
     9001 accgcaatct gttcatcatc ggtgccatcg ggctcggcat caccgtcgcc atcatcttgg
     9061 gtgcgatcaa ctacgacaag ctgccgttcc tcaaccaggg caaggagtat tcggcctact
     9121 tcgccgaggc gggcggactg atggacgatg ccgccgtaca ggtttcgggt ttccaggtgg
     9181 gcaaggtcga gtcgatcgag ctcgacggtc cgcgggtgct gatcaagttc accgtcgaca
     9241 agaacatccg gcttggcaac cggaccgagg cggccatcaa gaccaagggt ctgctcggca
     9301 acaagatcct cgaggtcacc tcgcgcggcg acggtcgcca ggaaggcaca attccgttgg
     9361 accgcaccac gtcgccgtat cagctgccgg acgcactcgg tgagttggcg accacgatca
     9421 gcggtctgaa caccaaccag ttgtcggatt cgctgcgggt gctggcgaca acgttcgccg
     9481 acaccccgcc cgagttgaag atcgcggtcg agggggtggc ccggttctcg gacacgctca
     9541 acgagcgcga cgcccagctg cgcggcctgc tgaccaacgc ggataaggcc acaacggttc
     9601 tggccgagcg cagcaatcag gtggtcagtc tgatcggtaa taccaactcg ctgctggtgg
     9661 agctccaaag ccaaagcgcc gcactggatc agatctcggg aaacatctcc gcgctcagtc
     9721 agcagctgca gggatttatc gcggagaacc gcgacacgat gaggcccgcg ctcgacaagc
     9781 tcaacggcgt gttgacgatg ctggacaatc gcaaggaacg cctgcagagg tcgatcaaac
     9841 tgctcactca gtactcgatg tcgcttggcg aatcggtggc gtccgggccc ttcttcaaga
     9901 cctatgtcgc gaatctgctg ccgggacagt tcgttcagcc gttcatcgac gccgcgttct
     9961 ccgacctcgg tctggatccc aatacgctgc tgccgtcgga acgtaccgac ccgcagatcg
    10021 gccagccggg gacaccggca ttgccggtgc cctatccgcg caccgggcag ggcggcgaac
    10081 cgcatctgac attgcccgac gcgattaccg gcaatcccgg cgatcagggg tgcggtccgc
    10141 ctggcttgcc gctgcccggc ccgacgggct gctaccccta ccgcgagccg ctcccagcgc
    10201 cgccgcccgg tggtccgccg ccaggtcctc ccgcgttggc gccacccggg ctggactcca
    10261 caccacagcc gacgccgagc cccgtcatgg tgcccgcgcc tggggaggct ggaccatgaa
    10321 ccgtattcgc aagagctggg ttgcggtcgc ccttgtcgtg ctgctggtcg ccggagtggc
    10381 cgtgctgttc cgcacgagcg acaccatcaa ccgcacgaat gtcgttgcgt acttcgagaa
    10441 cagcaatggc gtctacgtcg gcgacgacgt gcgcatcctt ggcgtgaacg tcggcaggat
    10501 caccgaaatc cagccgcaac ccgagcgggt caaggtctcg ttctggtacg acagcaagta
    10561 caaggtgcct gccgacgcca aagcggcgat cctgtcgccg acactggtca ccgcgcgtgc
    10621 catccaactc acgcctgtct acaccaccgg gccggtgatg cccgatgacg cggtgatccc
    10681 gcaagagcgg accgcggtcc cggtcgaatg ggaccaagtg cgtgcgcaat tggagaagct
    10741 ggcccacacg ctgcagccga ccgagcccgg cggagtcgcc ccgctgggtt cgctcatcaa
    10801 caccaccgcc gacaatctgc gcggccaggg tgccagcatc cgcgacaccg tcatcaagct
    10861 gtcgcaagcg ttttcggcgc tcggtgatca cagcacggac atcttctcga ccatcaagaa
    10921 cctggccatt ctggtatcgg cgcttcagga cagcaccaac ctgatgcgtc agctcaatca
    10981 gaacctggcg tcggttaccg gcctgctcgc ggacaacccc aacgaagtcg gcgacgcggt
    11041 aagggacctc aacgccgcgg tgggagaggt ccagacgttc gtggccgata accgcgaagc
    11101 gctgggcacc acgtcggaca agttggccgg ggtgacccag gcgttgaccg acagcctcga
    11161 cgacgtcaag caattcctgc acgtggcgcc caacacgttg cagaactacg tcaacatctg
    11221 gcaacccgcc cagggcgcga taagcagcgt gccgatgatc aacaacttcg ccaatccgat
    11281 ttcctttcta tgcggcgcta ttcaggcggc gtcacggctg ggcgcagagg agtcggcgaa
    11341 gctgtgcgtg caatacctgg cgccgatcat caagaatcgc caatacaact tcctgcccgt
    11401 cgcgcagaac gtcttcgtcg gtgctacgac acggccgaat gagctcacct acagcgagga
    11461 ctggatgcgg ccggattaca ttccgccgca accgactccg ggtgccgggc cacctgccgc
    11521 aacctcgcct gaggcagcac caccgcctcc cgggccgccg ttacctgcgg aggctccagt
    11581 cgctaccaat ccggccgacg gtctgaccgg aatgatggtg ccgccaggag ctggaccatg
    11641 atgcgcgcca agtggattcg tatcgcggtg agcttggccg tgctcgccct cgcggtgggc
    11701 gggtgcgccg actggcaagg gctgaactcg cttccgctgc ccggcgtgca gggtaccggc
    11761 cccggcggtt tcaccgtcca ggcgcagatg cccgacgtgg acaacatcga gcagaactcc
    11821 cgggtgcggg tcggcgacgt gaacgtcggc accgtcacga agatcgagcg ccagggctgg
    11881 catgcgctgg taacgatgaa gctcaacggc gatgtccaac ttcccgccaa tgcgaccgcg
    11941 aagctcggtc agaccagcct gctggggtcg ctgcatatcg agctggcgcc gccgaccgac
    12001 gtggcgccgc agggcacact gcgcgacggt tcgctgatac cgctggagtc ttccggcaag
    12061 tatccgagta ccgagcagac gctggctgca attgcgttgc tgctcaacgg cggtggtatc
    12121 ggcaacattc aagacatcac cgacgcgttg agtatcgcct tcaccggccg cgagaacgac
    12181 ttacgcagcc tgatcgagca actcgacaag gccatcggct acctcaacga ccaaaaacac
    12241 gacatcatcg cggcctcgga gagcctgaac aacctgatcg gtcagttcgc cgagcagaaa
    12301 cccgtggtgg acaaggcatt gaagaccatc ccagatgcgc tggtggtctt gaaggatcag
    12361 cgcgacaacc tctcggaagc gttggcgcag ctcggcaagt tcagcgcgct ggccgccgac
    12421 tcggtcaacc aaaccaagga agcactcgtc caagagctca aagaccttgg gccggtgttg
    12481 caatcactcg ccgacgccgg cccggcgctg acccgcgcac tgagcttcct gcccacattc
    12541 ccgttcccca aggagacgct gaccaactgg atgcgtggcg actacgccaa cctgacgttg
    12601 atcctcgacc tgacattgag ccggatcgac accggattct tcaccggatc gcggtgggag
    12661 tgcgatctga cctggctgga gattcagtgg ggccgcacca tcggccaatt ccccagcccg
    12721 tgcacgggga gcgccccgcc gaacggcgcc ggcaatccgt tgatcgctcc gtatcactgg
    12781 gatcaggggc cctgacatga ttttgacgag gcggatcctc atccagatgg cgatcttcgc
    12841 cgttgtggcg acgaccgcgc ttgcggtcat gttgttcggc tacatgcgcg ttcaggacat
    12901 ggcaggcatc gggcagtaca aggtaaccgt ccagctgccc gaaaccggcg ggctttatcc
    12961 gcggggcaac gtcacctatc gcggcgccca ggtcggcacg gtgaaaagtg ttcacctcac
    13021 cgattccggc gtcgccgcgg agctgtcgct ggactcgaat gtcaagatcc ccgccgatgt
    13081 cgaggccgaa gtacacagcg tgtcctccgt cggtgagcag ttcgtgcagc tgctgcccag
    13141 aagcggcaag gggccgacgc taaaggacgg cgacgtcatc ccggcggacc gcaccacggt
    13201 cccgaccgac atcaacaagg tcttggcgga cacgacccgt ggcctgcagg cgatcccgca
    13261 ggagaacctg aagaccgtca tcgacgaggc gtacgtcgcg gtgggcggcc tcggcccaga
    13321 gcttcgccgg ctagtcaacg gcagcaccaa cctggccatc gatgcgcgaa agaacctgga
    13381 cccgttgacc actctgatcg accagtccaa actcgtactg gatacacaga ccgagaccgc
    13441 ggggtcgatt caggcgtggg cgtcgaacct ggcaactatc accactcagc tgcagaacca
    13501 ggacgccgcc gtggccggag tcctggacaa ggggccgggt gcggccgatg aggtgcgggc
    13561 gctgttcgat cgcctgcagc cgacgttgcc gatcgtgctg gccaatctcg tcagcatcgg
    13621 ggaagtcgcc gtcacctacc aaccgagcct cgagcaactg ctggtgctgt tgccccaggg
    13681 caccgccgtc acgcaggccg tgggtgtgca caagcgcaac accaaacagg actacgaagg
    13741 cgatgccctg gtcttcaact tgaacctgtt gatccctttg ttgcctgcgc cgatccctct
    13801 tccgccgcag cagataccgc cgccgtgcac caccggcttc ctgcccgccc agcagcagcg
    13861 ggtgcccacc ttcgaggact atcccgacag gccaccgggc gatgtctact gccgggtgcc
    13921 gcaggacgcg ccgttcaatg tccgcggcgc ccgcaacctg ccgtgcgaga ccgtcccggg
    13981 caaacgtgcc ccgacggtga agatgtgtga gagcaacgag tactacgtgc cactcaatga
    14041 cggctacaac tggaagggtg acccgaacgc gacgctgtcc ggacaggcgg ttccgcagct
    14101 gccgcccggg tcaccacctg cgcaagccgc tccgccgccg ggtccgtcgc cgccgccaat
    14161 agcggccgcc gaatacgacc cagccaccgg cacgtacgtt ggaccggacg gacgtgtgta
    14221 cacacagtcc aacctggccc gcactgccac agaggagcaa acatggcaga cgatgctgct
    14281 gcccccgacg gggaattgag caagtcgacc gccaccgagg agacatcgag cgccaccgaa
    14341 ccggaatcgg gcggcagcga cgtctcggac gcggccgatt ccgacgcggc agtgacggac
    14401 gaggttacgg acgacgctga ctccgacgag gaggccgccc actccgagga ggaggctgac
    14461 tccgaggaaa ccaccgaagc cgcgccggcc aagccaccga tgtcgcatgt gaagctggcg
    14521 atgatcgcgg ggctggtgtt ggtggtcgcg ctcggcggac tgacggggtg gctgggttac
    14581 cgcgcctacc agtcgcatca ggccgatgaa caacgcaagc tcttcctgtc ggtgggcagg
    14641 cagggtgcgc tgaacctgac cacgatcgac ttccagcacg ccgatgcgga cgtacagcgg
    14701 atcctcgact ccgcgacagg cacgttttac gacgaattcc aaaagcgcgc gcagccgttc
    14761 gtcgaagtgg tcaagcaggc gcagtccaag tcggaaggca ccatcgccga ggcggggctg
    14821 gaatccgagt ccgacaaggg cgctcaggtg ttggtcgcgg tcaccgtcaa gacgtcgaat
    14881 gccggtgcgc ccgaccaggc gccacgcgcg tggcggatgc gcatctccgt acagaaggtc
    14941 ggcgatgacg ccaaggtgtc caacgtggac ttcgtgccat gagcaaccac agtcaagacc
    15001 agacggcaga agaggcgacc ttgacgtcgg acaccgagac cgagcaaatc gaggatgcgg
    15061 tttccgacga gaccgaaaag gccgacgagc caaccgaaac cgccgacgag gcaaccgagt
    15121 ccgctgaacc ggcgaagccc aagcagaaga tccaatggcc gcgggtgttc gcctacggtg
    15181 tgctgcccgc cctggcgctg ctgctggcga tgggcgcggg atacctgaaa tggacggaca
    15241 attcggtccg cgacgaccag accgcgcgta ttcaagcgat gcaggccgcc aaggacagca
    15301 cgatcgcgat gctctcgtac aagcccgaca ccgtggagca gcagctgacc gcggcccgcg
    15361 acttattgac gagtgacttc cgcgatcaat acacctcgct gaccaacgac gtcgtgatcc
    15421 ccggcgccaa gcagaagcag atctctgcgg tggcaacggt gccggcgatg gcgtcggtgt
    15481 cggccaaccc gcgtcacgcc gtggtcctgg tgttcgtcaa ccagaccgtg atcgtcggcc
    15541 aggacgcacc tacggactcg gcgtccagtg tgcgggtcac gctggacaaa gtcggcgatc
    15601 gttggctgat cgccaagttc gacccggtgt agcgtgaccg aaccccagtg gctcgacgtc
    15661 gcggcgccgg ccgtgcaact gcgcgcgctg acgtggggac cggccgacgc gccaattgcg
    15721 ttgtgcctgc acggtttccc cgatactgcc tacggctggc gcaaggtggc gcccgcgctg
    15781 gtggatgcgg gatggcgcgt ggtggcgccg ttcatgcgcg gctatgctcc gtcgtcgttg
    15841 ccgtcggacg gcagctatca cgtcggcgca tacatggatg acgcgctgcg ggtgctgaag
    15901 gccgcgggcc cgacggggcg cgacgtgttc atcggtcacg actggggtgc catcgcgggc
    15961 gccgggctcg cggcgatgcc cgacagcccg ttcaccaagg ccgtgatcat gtcggtgccg
    16021 ctcagcgccg cattccgtcc tttcggcagg gtgccgcagg ctggccggct ggctgcgaaa
    16081 ttgccgcgtc agctgctcaa cagctggtac atcctgtact tccaattgcc gtggctgcca
    16141 gaccgttccg cgtcgtgggt gataccacga ctgtggcggg attggtcgcc cggatatcgc
    16201 gccgacgacc atctgcgcta cgtcgacgct tcgatcggcg cgcccgaccg gtggcgcgcc
    16261 gcgctcggat attaccgcgc gacgatccgt ggcagccggc cgccggcgca gtacgcggaa
    16321 ctcaaccagc attggctgga cgcgccgcgg ttgccgacgc tgtatctgca cggcaccgat
    16381 gacggttgct cggaggacta cacgccgtgg attgagcgag tattgcccga cgagagcgag
    16441 atcgcgctca tcaagaacgg cgggcacttc ctccagctcg atcagcccga cgtcgtcggc
    16501 cgtcacatcg tcgacttcgt gggccaggcg cgctaaccct tcttcagccc gggatggttg
    16561 cgcgcgacca gccgcgtgac gagtcgccgc ggcaagtggt ggttgatcac ggcggcgatg
    16621 cggttggccg ccccgggaat gatcacacgc ttacccgacg ccaatccgtc gaccgcggct
    16681 ttggccaccg cgtcgggctc ctcccacaga aatttcggca gcgacttctc ggcttcttct
    16741 tgaggtatgc ccgcggcctc accgaaggtc gtccgcaccg gcccggggca cagcgcgctg
    16801 acggtgacgc cggtgccgcg cagctcttct cgcatcgcct cggtgtacga cagcacgaac
    16861 gccttcgccg cgccgtagga cgcttggcca ggtacggggc cgaacgcgcc gaccgatgcg
    16921 acgttgagca ccgctccccg tccgcgcttg accatgtcag ggacgaaccg gctgcagagg
    16981 tcgaccaccg cggcgacgtc gacctcgatc aggttgagtt cggcctccgg gtccgaatcg
    17041 gcgactgggc ccagtgtgga aagccccgca ttgttgatca ggacctccgc gacgacaccg
    17101 agcgccgcga cctggtcggg cagcgtggca cgctcgtcgc gattcgacag atcggcgggc
    17161 aggacgtgcg cccggccgtt gaattcttcc gccagggccc gcagccggtc ggctcgccgg
    17221 gccacgagga ccaatcggta accgcggcgg gcgaattcgc gggcgatctc ctcaccgata
    17281 cctgacgagg caccggttac gacgacgacg ccgtcgccgc ttataggcgg aagcatgggc
    17341 agacaatagc ctgagaccat gatggtccgt cggctctcgg cggtcgatgc gcaaacgtat
    17401 tggatgtcgg ccaagatccc caacgatcag ttcctgctgt acggcttcgc cggggtgccg
    17461 gacgtcgacc atgccgtcga tgtgatccgg cagcgctcgc gggactgccc ggagctgcgg
    17521 ctgcgggtgc gcgaccgagg cttcttcacc tatcccgaat gggtcgccgg tgaggtgcgt
    17581 ccgggccagt ttgtcgtgca cgacgtcgtc gacagcagct ggtcggagtg cctcgcggga
    17641 gtcgcccggt tggccgagga ccagctcgac ccgcaggcca tgacgtggcg gctgcatgta
    17701 ttcgcaggcg tcgacgtgcc gggtgccggt cgcggcagcg tcttggtgct gcaggcggcc
    17761 catgtgttcg gtgatggcat ccggtcgtct gcgttggcgg catggctgtt cggccgcaca
    17821 gagccagtgc ccgccgtcag ggtgccgccg ctacagacgc tgaggctccc gtggcgcagc
    17881 ttccgagcgg cccgcacgca tcgacagctc gttcgcgacg tcgacgccgg actcgttcca
    17941 ccgcaagcag attcgcgccc tgcactgcgc agcaacgcac gtccagatgg cgtgcgcagc
    18001 atccgcacgg tgatccggag ccgggctcag ctgcctggtc ccaccatcac cgtcggcgta
    18061 ttggcagcgg tgtcgacagc gctagcggct cacttgtgcg agctgggcga cgacccgtcc
    18121 gcgctgggtg ccgaggtgcc gatggcgaaa gccggtgtcc gtcaggcgca caaccacttc
    18181 ggcaacgtcg gtgttggcct gtatcccgac ctcgggtctg atgaacgggc ggcgcgcatc
    18241 gccgaggacc tggccgaccg ccgtcgcagg gcggcacacc cgggcatgcg tgcggccagc
    18301 cgggcgttcg ccgccgttcc tgcgccgttg ttgcggtggg gcgtcaagca attcgatccg
    18361 acggtgcggt caccggtcgt caccggcaac acggtcgtct ccagcgtcaa ccgcggcccg
    18421 gccgacctgc gcttcggcga tgcgcccgtg gtcctgaccg cgggctatcc cgggctgtca
    18481 ccgatgatgg gactgacgca cggcgtgcac ggcatcggcg acaccatcgc gatcagcgtg
    18541 cacgccgcgg agtcggcgat cggtgacgtc gacgcctacg tcgagcggct cgaggccgcg
    18601 ctgggttagc cgcgcagcag cccgaaaccc cagttggtac cgaagccgcg ccgtagcgcg
    18661 aggtggatcc agaccaatgc gaacggctcc agcagccggg ccaaggtgag gtcgcccgcg
    18721 tcgacggcct caaagcccag ctgcgtcacc aaatcggcgg tgacctgctt ggccgcgtcg
    18781 tcgtcgccgc agacgaacat gaccggcgcg cttggcaatt ggggatggtc catcatcggg
    18841 gcaccgatct gattcatcgc cttgcacacc cgcgcgcccg gcgcccaggc cgcgacctgc
    18901 tcggcgcccg aggtggtgtg tccaatctcc agcgctgaga agtcaggggt gagcgggttg
    18961 acgcaatcga tgagcacctt gccggtgagg tcgccgcacg ccttgagcgc cgcctcggcg
    19021 ccttgccacg gcgtgcagag cacgacgacg tcgctgcctg caaccgccgc gtcattggtg
    19081 gtagctggcg caccaaggtc ggcgtacttg gggtcgttgg gggagcgcac gccgtaggtg
    19141 atgtcgtggt ctgtccgcca tgctcgaccg agcgcacgtc cgacgtttcc cgctccgata
    19201 atcgcgattt tcacgagggt tacgctacgg cggaaggcgg acaccgataa gtacgcactt
    19261 tgaggtgagc tactagccgg gaggtcggta tggacgatca gttcggatgt ccagtggagg
    19321 ccacgctcgc ggtcgtcggg ggtaagtgga aagccgtgct gatcttccac atgatgcaca
    19381 ccggaccgca ccgcttcgcg gagttgaagc gcaagacgac cgggatcagc gaccgcgtgc
    19441 tgacccgtca gctgcgggag ttggagaccg acgggattgt gcgccgcgag gtgttcgccg
    19501 aggtgccgcc gcgcgtcgag tactcgctga ccgaatacgg cgaatcgctg cggccggtga
    19561 ccgaggcgat gtgccagtgg ggcaagcgcc acatgagcac tggggccgcc tagcattctt
    19621 ccggcgaaca gacgcaaacc tgccctcttt tcgcgaaatt ggggcagttt tgcgtctgct
    19681 cgacctagtc gctggcgcgc agtgactcga tgccgacgtc gagcgcagct tccagataac
    19741 tgaggtcgcc ggtggcgagc atgacaccga cgccgccctg gacggctgcg agcagcgcgg
    19801 cggcgattcg gtccgcatcg agccgggcgg cgaccttccc gtctgcctgc atatgtcgca
    19861 cgcccgctgc gatctcatcg gtccacttgc gaaggagcgt cgacgtgacc gcctgagcgc
    19921 ccggtgtgct gcggccgatc tcggacatca gcacggccag tgggcagtgt tggccctggc
    19981 ggcggtaccg atcgacgacg gcgtcacgcc accgctgcca ggccgcccac gacgtcaact
    20041 cgcccagata tggctgctgg tcatcgagga ccatctgcgc ttcatgctcg gcgaccgcga
    20101 gtaacagctg gtccttgccg tccgggaagt aatgaaacag ttggcttttg gaggtctggg
    20161 tgcgcgcgcg aatgtcgtcg agtgtggcga cggcgacgcc atgttcacgg atttcggctg
    20221 cggcgccctc gatgatgcgc tgccgcgtcg cttggccttt cggcgtcaat ttctggactt
    20281 gcaggtccat ttttgccagc gtaccagtgg acacatgagt tcaaacaccg cgcggctcgc
    20341 cggccgcaca gcactggtca ccggttccac gggcggtctc ggggtcgcca tcgcctcggc
    20401 actagcagcc gagggcgcgt ccgtcatcgt cagcggccgc gacaaggccc gcggcgacgc
    20461 cgtcgtcgcc gacatccgat ccgctggtgg cgcggccgag ttcgtcgtcg ccgacctggc
    20521 cgcgggagat caggaggtac gtcgccttgc cgaacaggcc accgctgcgg cgggagggcg
    20581 gatcgacatc ctcgtgaaca acgccgcgat gctgctgatg ccgacgccga ccgccgaggt
    20641 ctccgacgaa gtgctgcgag atgcgttcgc ggtgaacgtg tttgcgcctt tcctgctcac
    20701 cggacaactg gcgcccgcga tggtgcagcg cggtgagggc gccatcgtca acatcggatc
    20761 gatcacgggg ttgcgcggca gcgcgggctc ggcgatctat tcggccaaca aggcagcgct
    20821 ccactcgttc acccagtcgt gggccgacga gtatggcccc tccggggtgc gggtcaacgc
    20881 cgtcgccccg gggccgatcg ccaccgaacg ccaggagcag ttcgccgacc acgtcgcccc
    20941 gatggtggct cgcatcccgt cgcggcggat gagcaccccg gccgaggtcg cagcctcggt
    21001 ggtgttcctg gccagcgacg acgcggcaaa catccacggg accgtgctag gtgtggacgg
    21061 gggttgggcg gcggtttaac acatggccta gattttgttg catgactgac cacacggcca
    21121 tcgacaccgg gctgctgata ctgcgggtgg tgctgggtct gaccatggcc gcacacggct
    21181 acaacaagtt cttcggcaag ggcggcttgg cgggcaccgc gggctggttc gacagcatgg
    21241 gcatgaagcc gggcatgttt cacgcccgga tcgccgctac caccgagatg tcggcgggcc
    21301 tcggtttggc ggccggcctg ctgactccga tccccgcggc gggcttcgtc gcgctgatgt
    21361 tcgtcgcggc gtggaccgtg caccggccga acggcttctt catcgtcaag gaaggctggg
    21421 agtacaacct ggttcttgcg gtgtcggcgg tggcgatcgc cacgatcggg ccaggcaagt
    21481 tcagccttga ctacgcgctc ttccgcacga cgggcctcta cgacttcctg cacggctggt
    21541 ggggtctgct gatctcggcc ggactggggc tggccggcgg catcggccag ctggtgatct
    21601 tctggcggcc accggcgaag gccgatgcct agctcgccag cccgagcaga acgtcggcca
    21661 gaactgaggg ccgtgagtag aacggcgagt ggtcccctgg cagctcgatg agctcggcgc
    21721 ccaaccgttc gcgagcggtt tgccttgacc atgcgggacg caggatctga tcgtcgctgc
    21781 acgcgacgta tgtcgttttc acagcgggga attcggccag tgaggacggc aggatcgccg
    21841 ggtatgccga ctgaggtcga agacgctcga gggcggctct ggtggtcgcc tcgtcgcagt
    21901 cgccgtagaa catcgcgcac gcgaggtcga ggtccgtcca cacttgccgc agctgctcat
    21961 ccggcacgct caaaccttgt tcgtacgcca ccgggttcag catctcgagt tcgtcactca
    22021 tctgatcgaa cacgctgcgg ccgatgtcgg ggaccatcgc gcacagatac accagatgcc
    22081 ggacggatcg acgcgcggcg accaacggga tcgtgtttcc gttgtaggag tgtccgacca
    22141 gcacgacatc gtcgccgcga ctatccagtg ccgcgcacac cacgtcggcg taggtgtcga
    22201 aggtggccgt tccgtcctcg ttgggcaggt ccatcgtcac cacgtcatgg ccggcttgtc
    22261 gcaggagcgg ggtgagctgc tcccaacacc acgcgccgtg ccatgcgccg tgcaccagcg
    22321 cgaaggtggt catccggtaa tgctaaagcc attccactcg gctagaacac gttctagtct
    22381 catgcgtcat ggggtttctg aagcaggacg cgcccgtcgt cgactacgag gagtggagca
    22441 agggcagccg cgccgaaagg atcgttccga tggccaagca ctgggccgag gtcggattcg
    22501 gaactccggt cgtgctgcac ctgttctacg tggtcaagat cctgctctac atccttggcg
    22561 ggtggctgtt cgcactggcc accgaaggca tcgacgggtt caccaacgtc gcgtcgtggt
    22621 acgccgagcc gatcgtcttc gaaaaggtcg tgctctacac gatgctgttc gaggtcgtcg
    22681 gcctcggctg tggcttcggt ccgctgaaca accggttctt cccgccgatg ggctcggtcc
    22741 tgtactggtt gcggcccaac accattcggc ttccgccgtg gcccaaccgg ataccgctga
    22801 cgagaggcac cacccgcaca cctgtggacg ctgcgctgta cggtgcgctg ctcgtgatgc
    22861 ttctggtcgc actgttctcc gatggcaccg ggccgatccc cgcattgggc acgacggtgg
    22921 gcgtgctgcc aatgtggcag atctggacga tcgtcggtct gctggcggtg ctcggactgc
    22981 gggacaaggt gatcttcctg gccgcgcgcg gcgaggtgta cgcgtcgttc acggtcgcgt
    23041 tcctgtttgc cggctacggg gtcgacatga tcatcgccgc caaactggtg tgcttggtga
    23101 tctggctggg cgcggcgacg tccaagctca acaagcattt cccgttcgtg atctcgacga
    23161 tgatgagcaa caacccggtg ttccggccgc ggttcatcaa acgcaagttc ttcgaacatt
    23221 tccccgacga cctgcggccg ggccggctgt cgcggtggct cgcgcacttc agcaccgcga
    23281 ttgaggggct ggtgccgctg gtgttgttct tctcccacgg cggctggccg accgcgatcg
    23341 ccgcgttcgt catgctgtgc ttccacttcg gcatcctgtc gtcgattccg atgggagtgc
    23401 cgctggagtg gaacgtcttc atgatgttca gcgtgctcgc gctgttcgtc ggccacgccg
    23461 aagtggggcc gggcgacatg acgacgccgc tgccggtgct gttgttcggt gtcgtcgcgg
    23521 gcaccgtggt gatcggaaac ctgttcccgc gcaaggtgtc gttcctgccg ggcatgcggt
    23581 actacgccgg caactgggac accgggttgt ggtgcgtcaa gccgtcggcc gccgagaaga
    23641 tcgaggccaa cgtcgtgtcg atcgcgagca tgccgcaggc gcagatggag cggtactacg
    23701 gcagcccgga gaccgcgcag atgtacctct atatgggata tgcgttccgc tccttcaaca
    23761 ctcacggccg tgcgatgttc acgttggcgc accgcgccat ggccggacag aacgaagacg
    23821 actacgtgct gaccgacggc gagcgcatct gttcgacggc catcggctgg aacttcggcg
    23881 acggccacat gcacaacgaa cagttgatcg aggcgctgca ggagcgctgc cacttcgaac
    23941 cgggggagat ccggatcctg ttgctcgatg cgcagcccat ccacaagcag cgacaggagt
    24001 accggctcgt cgattctgcc accggcgaat tcgaaagggg ttacgtcaaa gtcgccgaca
    24061 tggtgacgcg ccagccgtgg gacgacaccg tcccagtaca tgtgacgtgg gagaaggaca
    24121 gcgctgacgc cacctgatcc ggtgtctgtc tggttcgacg agaccggaca gttgatgagc
    24181 gaccttgggc ccgttgacag gaactgcgtc gtgcgggtga tcagcgggca ttgccccgat
    24241 cgacatcagt gtgtgctgct ctaccgggcg ccgggcccac ggctgctcta cggcagcgag
    24301 ctgatgtcgg aactcgacga cgaaagaggt ctctacttgg agacccacgc caaacacctt
    24361 gaagacgacc tcatttcgat cacggtcgac catgtcgggg tcgacgggca cgcggacggc
    24421 tggcgctacc gactgttgcc gatgcggtgg aagacggagg acgggctggt ggacacccca
    24481 acgcgcctgg cggtctggcc ggactaaggg gccacacgcc tcgagactgc ccacagatcg
    24541 caatctcggt cgaaaacgcg atctccccgc agtttcggcg aaggggacta gc
//
LOCUS       DASRJA010000021        12742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3928313,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000021 DASRJA010000000
VERSION     DASRJA010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3928313"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFC01_00945"
     CDS             <1..308
                     /locus_tag="VFC01_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN78243.1"
                     /translation="GWYLLYRYWPTSHNTHKFEAYNAFHPARTVRERVEHEVASVVLK
                     EFALQDAGMLGGTQAALEYDVVNDFPLSDQEILVRHLHKVAVDWVQQYQRERSPVEV"
     gene            311..646
                     /locus_tag="VFC01_00950"
     CDS             311..646
                     /locus_tag="VFC01_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78244.1"
                     /translation="MSDKRLPSAFAEFEPFADKWCLPTEPERWAARLSTPMPEIREFY
                     DAFTPRFEEAIDYCDKFPLDDVPDDALNLLHLVYSMIMVSMAVEVFGQQKPTDSADAV
                     IDRIGEPVP"
     gene            643..1488
                     /locus_tag="VFC01_00955"
     CDS             643..1488
                     /locus_tag="VFC01_00955"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZN78245.1"
                     /translation="MSLLTINKLTESVGAEVLGVDSDRLADDELLAAAVLDALEENGV
                     LVFPRLGLNPQAQVAFCRRLGEIDHSSDGHHPIAGIYPITLDKSKNASASYLRATFDW
                     HIDGCTPMKDEFPQKATVLSAVQVAERGGETEFASAYVAYDELSADDRQRVASLRVVH
                     SLEASQRRVNPDPSPQELARWRARPTHEHPLVWTHRSGRKSLVLGASADYVVGMAPDE
                     SAALLADLLDRATSADKVYSHTWSVGDTVIWDNRGVLHRAAPYAPDSQREMLRTTVLG
                     DEPIQ"
     gene            1596..2879
                     /locus_tag="VFC01_00960"
     CDS             1596..2879
                     /locus_tag="VFC01_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN78246.1"
                     /translation="MAEDLTSVDFFRDGRLTDDPYPFYEALRNKCPVSREEHYGVTMV
                     TGWQEAVDVYNDEQTFSSCISVTGPFPGFPVSLEGLADGDITELIEQHRDEIPFSDQL
                     PTLDPPTHTNHRALLMRLITPKRLKENEDAMWQIADDMLDTFLAPGEGEFINGFAGPF
                     TLRVIADLLGVPDEDRGELIERLARGTHGGGLGNAEKSLNKTPLEYLYDIFATYVEDR
                     RAEPRDDVLTGLATATFPDGSVPDVGDVVRVATNVFSAGQETTVRLLGTALKVLGDRP
                     DIQRKLREDRSLLPNFIEECLRIESPVKGDFRLSRVPTTVGDQALAAGCTVMVINGAA
                     NRDPRRFEDPDSFDAERKNARQHLAFGRGIHSCPGAPLARAETRVALERLLDRTTDIR
                     ISEKHHGPASDRRYQYIPTYILRGLTELHLEFDLV"
     gene            2876..3067
                     /locus_tag="VFC01_00965"
     CDS             2876..3067
                     /locus_tag="VFC01_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN78247.1"
                     /translation="MKVKVDEDRCRGHGMCLTLCPDVFHMTDDGYAVADPSEVPAGLE
                     SAAQDAIANCPEQAISEID"
     gene            3084..3368
                     /locus_tag="VFC01_00970"
     CDS             3084..3368
                     /locus_tag="VFC01_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HZN78248.1"
                     /translation="MPKGYIIITEDVKDPAGMAEYGKLASKAMAGATLLSFDSKPEVL
                     EGEWHGTQTVLLEFDSVEAAREWYNSDSYQEAAKLRQAAADCNGVIVAGL"
     gene            complement(3668..4012)
                     /locus_tag="VFC01_00975"
     CDS             complement(3668..4012)
                     /locus_tag="VFC01_00975"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN78249.1"
                     /translation="MMSARHNITKLVGAAIAVAAVGLGTPGIAAAYTPEDLALFKKLY
                     ADGVDFSEANQVIQRARTVCDRFAAGDSPVEVHEAVITNSAFSPRQAAIFMADAVQAY
                     CPQFSGQFMTSS"
     gene            complement(4324..4524)
                     /locus_tag="VFC01_00980"
     CDS             complement(4324..4524)
                     /locus_tag="VFC01_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN78250.1"
                     /translation="MTKKVVVDFGLCESNGVCMGIIPEVFDLDDQDYLHILQEEVTPE
                     NEHQIREAVRQCPRQAISISDE"
     gene            complement(4553..5755)
                     /locus_tag="VFC01_00985"
     CDS             complement(4553..5755)
                     /locus_tag="VFC01_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN78251.1"
                     /translation="MTKPKLKFNPVAQEYFDNPYEIYKRMRDEAPIYYDEDEDFYALT
                     RHADVAAALKDYESFSSSRGCDLGMVRTGEVPQKSIIFMDPPDHRHMRSLLNKAFTPR
                     AIQSQRDTVVELVEHYLSKVDPDNFDVVQDFSGPFPVEVITRMAGVPEDFRQQVRHWI
                     DTSLEREPGQIELSEKNMQANIDSAMYYYSLVQERRESPQDDMISRLIAAEIPDDEGN
                     LRKLDDIEITGFTTLLGGAGAETVTKLVGSAVVEFAKHPEQWQMLLDDRSKLPDAVEE
                     LLRYVGPVQYNVRYTLKEAHVPSGTVPAGKPVFLMKAAANRDPRAYDRADEFDITRDR
                     TQAPHMGLGYGIHSCLGAALARLETTVALEHLLDFMPRYEVNFDGLQRVTMQNVAGYH
                     NVPVKVLK"
     gene            complement(5819..6637)
                     /locus_tag="VFC01_00990"
     CDS             complement(5819..6637)
                     /locus_tag="VFC01_00990"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN78252.1"
                     /translation="MSRVGFVGAGRMGAPMVRRLVGAGHEVRALGRSVEKRRAVSELG
                     ARPVTDLADIGAEADVVIVCVFTDGQVQQVCLDSVLMSTMQSGASIVIHTTGSPRTAE
                     AIASEAAAHGIGVVDAPVSGGPHDIAAGQVTLFVGGADDVVERVRRVLAGYGDPILHV
                     GPVGAGQAVKLVNNTLFAAQIGLLQEAVRLGKHLGVDEKRLLDATGHGSAASRVASIV
                     GARGSVDAFIDMVGEFVGKDVAVVRKIAAELGSDLGLLDDAINAGSKVSGQRNC"
     gene            complement(6634..7452)
                     /locus_tag="VFC01_00995"
     CDS             complement(6634..7452)
                     /locus_tag="VFC01_00995"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN78253.1"
                     /translation="MRVGFIGLGSQGGPMARRIVEGGFETTLWARREASLEPYADTAA
                     KTAGSPAELAAASDLVCLCVVGDDDVREVLYGDTGVLAGLASGGTVAVHSTVHPDTCK
                     EIAEKAAAQGVSVIDAPVSGGGPAVEEGKLLVMIGGDEDVVERCRPVFATYADPIVHL
                     GPLGSGQVTKILNNLLFTANLGSAITTLELGDALGIPRDKLCEVLNRGSAVSKALGSI
                     AVFGGTLDGLAPIAGGLLQKDVRHAASLASVASAPEGAVFDAADAALKSMDHPR"
     gene            complement(7452..8339)
                     /locus_tag="VFC01_01000"
     CDS             complement(7452..8339)
                     /locus_tag="VFC01_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN78254.1"
                     /translation="MSPRPRVVLVDGVPMSGLVALADEPKAVVVAFHGGASTAAYFDC
                     PGHPDFSLLRTGAANGYTMVALDRPGYGASAPYPDAMAHPEQRVALTYGAIDTVLGGN
                     PRGAGLVLLGHSAGCELAVRLASDDRAKDAGVIGLELAGTGVEYHPDTAEILKTATAT
                     RRPKGLRDILWQPSHLYPDDVLSGMTNSTSGALYEVDMTQRWPRQYFPELAARIAVPV
                     QFTVAEHERVWRSDAETLKHIEAMFSLSPRFTVNEQAGTGHNLSLSLSAAAYHAEVLS
                     FVGECVAAQQGTSKEREAG"
     gene            complement(8336..9979)
                     /locus_tag="VFC01_01005"
     CDS             complement(8336..9979)
                     /locus_tag="VFC01_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZN78255.1"
                     /translation="MTRPLPELTLQNEFFWTAGGDGVLRIQECRDCEALIHPPQPVCR
                     YCRSRNMGVRDVSGKATISAFTVNHRFGFPDLPPPYVVAQVAIVEDPRVRLTTNIVEC
                     EHDDLTIGQAVEVTFEKIEDVWLPLFRPSSETEAGPQAVDEIAPQDFGKHVAPMLTKE
                     KFEDTAAITGIGMSKIGRRQMLPPLSLTIDACEQAVADAGLTFDDIDGLSTYPGLDIA
                     GMGEGGVSALEGALGLRPTWINGGMDTFGPGGSVIAAVMAIATGMARHVLCFRTLWEA
                     TFQQLMKEGRTSPPGGARTSSWQMPFGATSAAHTLALNAQRHFARYGTTRETLGWIAL
                     NQRANAALNPTAIYRDPMTMADYLNARPITTPFGLYDCDVPCDGAIAVVVSAVDVAKD
                     MPRKPVLFEAVGTQIVERTDWDQTTLTHEPQVLGQAAHLWTRTSLQPKDVDVAELYDG
                     FTFNCLSWLEALGFCGIGEAKDFLDGGKAIARDGVIPLNTHGGQLSHGRTHGMGLIHE
                     AVTQLRGDAGERQVKDARVAVVSSGGLTPSGAILLRTDT"
     gene            complement(9976..10281)
                     /locus_tag="VFC01_01010"
     CDS             complement(9976..10281)
                     /locus_tag="VFC01_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN78256.1"
                     /translation="MSDGLRIRLDRTLCDGFGICAKHAPEYFSLDDWGYASLIGDGTV
                     PEKDRDAVMRALFDCPVHAIIYMGEHRPTGDRAVHHDAQESPEPEPKDGEAISEFVR"
     gene            complement(10278..11546)
                     /locus_tag="VFC01_01015"
     CDS             complement(10278..11546)
                     /locus_tag="VFC01_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HZN78257.1"
                     /translation="MTSTATELIVAAWPNCEPRLLRPSAGSDYLPLDDAEALLEQVDL
                     SGLLGRGGAAFPLGTKLRTVRDAGRRGSETVVVANGEEGEPASVKDRWLLRNRPHVVL
                     DGLRLATAIIGATRAYVYVSDQQSATAVASALEELKPETFGDTAVSVVTVEPTYVAGE
                     ETATVRRINGGPAKPTDKPPRPFEEGVFGLPTMVSNVETLANLPFIHAHGAQAFRAVG
                     TPMSPGTFLATITGAGRPAALYEIPHGTAFSELLSVHGLSAESVRGALMGGYFAGLVN
                     TDILDATLDHETIRRLGSGLGCGAISLLTDDCPVAVAASVLSYFDRENAGQCGSCFNG
                     TAAMAAVASALRDGVATDEDLARLERWSVVLRGRGACGTLDGATNVAASLLRQFPQLV
                     AQHLANDCPSCRTAAFDALRPYEVEAVGHS"
     gene            11580..12053
                     /locus_tag="VFC01_01020"
     CDS             11580..12053
                     /locus_tag="VFC01_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78258.1"
                     /translation="MRTAVVRVGVDRAGELAPAQLRDGMARLGQLAGPAGFEVVDNDL
                     VGLPPQRREVELLIAGDDPADLERRAVELCAKAFGTTPVPGVLTFISRGTDADAHGVL
                     AGLGLSGEIERVAGDEGWDVVHVTLRKADLERVPESRVHTALEASLNCEVHIRTI"
     gene            complement(12050..>12742)
                     /locus_tag="VFC01_01025"
     CDS             complement(12050..>12742)
                     /locus_tag="VFC01_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN78259.1"
                     /translation="NCGKSDVQFYGQSESHMRAETLHALVTALGIGPCIIAGGSGGAR
                     DSMLTIMLYPEIATKLVVWNIVGGVYGTFVLGAHYITPNIRPVLTMGMEGLLHLREWK
                     ERIADNPANEARLLALDPDEFVKVMMRWLNAFVSKPGQTIPGVEDEMFDNITVPTLII
                     RGGEDDWDHPKRTSLEVSCLIKGSELIDPPWPEDAWEQAAKDNAQGKVKHFNMFDTWV
                     QAAPAILKFLDG"
BASE COUNT         2167 a         4138 c         4168 g         2269 t
ORIGIN      
        1 acggctggta tctgctgtac cggtattggc cgacgtcgca caacacccat aagttcgagg
       61 cgtacaacgc ttttcatccg gcccgcacgg tccgcgaacg agtcgaacac gaggtcgcat
      121 cggtggtgct caaggagttc gcgttgcagg acgccggcat gttgggcggc acacaggccg
      181 cgctggagta cgacgtcgtc aatgacttcc ccctcagcga tcaggagatc ctggtgcgcc
      241 acctgcacaa ggtggccgtg gattgggtcc agcagtatca gcgcgagcgc agcccggtgg
      301 aggtgtgacc ttgtccgaca agcgacttcc cagtgccttc gcggagttcg aaccgtttgc
      361 tgacaagtgg tgtctgccaa ccgaacccga gcggtgggcg gcgcggctgt cgacgccgat
      421 gcccgagatc cgcgagttct acgacgcctt cactcctcga tttgaggagg cgatcgacta
      481 ctgcgacaag ttcccactcg atgatgtgcc cgacgatgcg ctgaacctgc tgcacctggt
      541 ctactcgatg atcatggtct cgatggccgt cgaggtcttc gggcagcaga aaccgaccga
      601 ctccgccgat gcggtcatcg atcggatagg ggagccggtg ccatgagcct gctgaccatc
      661 aacaagctga ccgagtccgt cggcgccgaa gtgctcggtg tcgactctga ccggttggca
      721 gacgatgagt tgctcgcggc cgcagtgctc gatgcgctgg aagaaaacgg cgtgctggtg
      781 ttccctcggc tcgggctgaa tccacaagcg caggtggcgt tttgccgccg gctcggcgag
      841 atcgaccact cctcggacgg acaccacccc atcgctggca tctatccgat caccctggac
      901 aagtcgaaga acgcgtcggc gtcctaccta cgtgcgacgt tcgattggca tatcgacggc
      961 tgcactccta tgaaagacga gttcccgcag aaggcgacgg tgttgtccgc ggtccaggtc
     1021 gcggaacgcg gtggcgagac cgagttcgcc agtgcctacg tcgcgtacga cgagttgagc
     1081 gctgacgaca ggcaacgggt cgcatcgctt cgcgtcgtgc attcactcga agcctcgcag
     1141 cggcgggtca atcccgatcc cagtccgcag gagttggcgc ggtggcgggc ccggccgacg
     1201 catgagcacc cgctggtgtg gacgcatcgc agcggtcgca agtcgctggt gctgggggcg
     1261 tctgccgact acgtcgtcgg catggcgccc gatgaaagcg cggcgcttct ggcggaccta
     1321 ttggatcgcg ccaccagtgc ggacaaggtg tacagccaca cgtggtccgt cggcgacacc
     1381 gtcatctggg acaaccgcgg tgtgctgcac cgcgcggcgc cctacgcccc ggattctcag
     1441 cgggagatgc tgcggaccac cgtgttgggg gacgagccga ttcagtaatg cctggtcgga
     1501 ccccgtcgtt gacggcggcg agccactatg gaaatctaat acccgcatat gagaatcacg
     1561 ttctctcatg cagagatcgt tgaaacggag caagcatggc ggaagacctg acctcggttg
     1621 acttcttccg ggacggccgt ctgaccgacg acccgtaccc gttctacgag gcgcttcgca
     1681 acaagtgccc ggtcagccgc gaggagcatt acggcgtgac gatggtgacg ggctggcagg
     1741 aggccgtcga cgtctacaac gatgagcaga cgttctcgtc gtgcatctcg gtgaccggcc
     1801 cgttccccgg cttcccggtg tcgctcgagg gcttggccga cggggacatc accgagctga
     1861 tcgagcagca tcgcgacgag attccgttca gcgatcagct tccgacgctg gacccgccga
     1921 cgcacacaaa ccaccgcgcg ctgctgatgc ggctgatcac acccaagcgc ctcaaggaga
     1981 acgaggacgc gatgtggcag atcgccgacg acatgctgga tacctttcta gcgcccggtg
     2041 aaggcgaatt catcaatggc ttcgcgggcc cgttcaccct gcgcgtcatc gccgacctgc
     2101 ttggggtgcc cgacgaggac cgcggcgagc tgatcgagcg tcttgcgcgc ggcacccacg
     2161 gcggtgggct cggcaatgcc gagaaatcgc tgaacaagac gccgttggaa tacctctacg
     2221 acatcttcgc gacctacgtc gaggaccgtc gtgcggagcc gcgcgacgat gtgctgaccg
     2281 gactggcgac tgcgaccttc ccggacggat cggtgcctga cgtcggcgac gtcgtgcggg
     2341 tggcgaccaa cgtgttctcc gcagggcagg aaaccaccgt gcggttgctc ggcacagcgt
     2401 tgaaggtgct cggcgaccgt cccgatattc agcgcaagtt gcgcgaggat cgcagcttgc
     2461 tgccgaactt catcgaggaa tgcctgcgta tcgagagtcc ggtgaagggc gacttccgat
     2521 tgtcgcgggt gccaacaact gtcggcgatc aggcgctggc tgcggggtgc acggtgatgg
     2581 tgatcaacgg ggcggccaac cgcgacccgc gccgcttcga ggatcctgac agcttcgacg
     2641 ccgaacgcaa gaacgcgcgt cagcatttgg cgttcgggcg cggcatccac agctgccctg
     2701 gtgccccgct ggcgcgcgcg gaaacccggg tcgcgctgga gcgactgctg gatcgcacca
     2761 ccgacatccg gatcagcgag aaacatcacg gcccggcttc tgaccgccgc taccaataca
     2821 ttccgacgta catcctgcgt gggctgaccg aattgcattt ggagtttgat ctcgtatgaa
     2881 ggtgaaggtc gacgaggacc gctgccgggg ccacggcatg tgcctgacgc tatgccccga
     2941 tgtgttccac atgaccgacg acggctacgc cgtcgccgat ccgtccgaag tgcccgccgg
     3001 gctggaaagc gccgcgcagg acgccatcgc gaactgccca gaacaagcca tcagcgaaat
     3061 cgactgaaac acaaggagtc tcaatgccca agggctacat catcatcacc gaagacgtca
     3121 aggatccggc agggatggcc gagtacggca agctggcgtc gaaggcgatg gccggggcga
     3181 cgctgctgtc gttcgactcc aagcccgagg tgctcgaggg cgaatggcac ggcacacaga
     3241 ccgtcctgct cgaattcgat tcggtcgagg cggcccgcga gtggtacaac tccgactcct
     3301 atcaggaggc ggccaagctg cggcaggcgg cggccgactg caacggcgtc atcgtcgcgg
     3361 ggttgtaact ttcctgcccg tccggctggt cacgtcccgc ggctgaggtg tgtggcgatc
     3421 cgtgggatga ggtctaacgg aattcggcgc acgtcatcga caaccgtggg gatgcgttgt
     3481 cgcatcgggc gtcgcgtgcc gcttaacttt cgtactccgc gaccaccatc gacgaaggcg
     3541 agctcgacca acgtggctgc cgcggtgacg agtcagcgtc cgcctgcagt atcaccggcg
     3601 gccgggccta tagtcaccaa cgctgtcgag ccgcgtgtaa cccctgcacc gcaatggttt
     3661 ttcaagctca cgacgaggtc atgaactggc cgctgaattg gggacagtag gcttggaccg
     3721 cgtcggccat gaatattgcg gcctggcgcg gactaaatgc agagttggtt attactgcct
     3781 cgtgaacttc caccggcgaa tcgccggcgg cgaacctatc gcagactgtc cgggcccttt
     3841 ggataacctg gttcgcttcg ctgaaatcca cgccgtcggc gtagagcttt ttgaacaacg
     3901 cgaggtcttc aggcgtgtag gcggcggcga tgcccggggt ccccaggcca acggcggcta
     3961 ccgctatcgc agcgccgacg agcttggtga tgttatggcg agcggacatc atgacgcctc
     4021 cactagaccg acccagtctt gtactgaaca ttatcttccc actgctatga gctgcgaaac
     4081 tggagtcatt ggtccgctat cagcagagcg actgtggata accgtgcaga cgcagaggac
     4141 ccgacacggc gcggatttgg gcccttgcgt ctgctcgcga gcgttaatgg cgcaccaagg
     4201 ggccatgcgg cgtgaaaacc gccgtgcacg aggacgaatc gcattgcgct gaatctccgc
     4261 tcagatcgac gttttggtgt tcgacactcg caactctccg ctcaaacgtc cgtttcggcg
     4321 tagttactcg tcagatatcg aaatggcctg ccgcggacac tgacggaccg cctcccggat
     4381 ctgatgctcg ttctccggcg tcacttcttc ctgcagaatg tgcaggtagt cctgatcgtc
     4441 gagatcgaac acctccggga tgatgcccat gcacaccccg ttgctctcgc acagcccgaa
     4501 gtcgactact actttcttgg tcatccgcga tcccgcttcg cttctcgctt ggtcacttca
     4561 gcaccttcac cggcacgttg tgatagccgg cgacgttctg catcgtgacc cgttgcagcc
     4621 catcgaaatt gacctcatag cgcggcatga agtcgagcag gtgctcgagc gcgacggtcg
     4681 tctccagccg ggccagcgct gcgccaaggc agctgtggat cccgtagccg agacccatgt
     4741 gcggcgcctg cgtgcggtcc cgggtgatgt cgaactcgtc ggcgcggtcg taggcgcgcg
     4801 ggtcgcggtt ggccgcggcc ttcatcagga acaccggctt gcccgcgggc acggtgccgc
     4861 tgggcacgtg ggcttccttg agcgtgtagc gcacgttgta ttgcaccggg ccgacgtaac
     4921 gcagcagctc ctcgacggcg tcgggcagct tgctgcggtc gtcgagcagc atctgccatt
     4981 gctcgggatg cttggcgaac tccaccaccg cgctgccgac cagcttggtg accgtctcgg
     5041 cgcccgcgcc gcccaacagg gtggtgaagc cggtgatctc gatgtcgtcg agcttgcgca
     5101 gattgccttc gtcgtcgggg atctccgcgg cgatcaggcg gctgatcatg tcgtcctgcg
     5161 ggctttcccg tcgctcctgc accaggctgt agtagtacat cgcggagtcg atattggcct
     5221 gcatgttctt ctcggacagc tcgatctggc cgggctcgcg ctccaggctg gtgtcgatcc
     5281 aatgccggac ctgttggcgg aagtcctcgg gcacaccggc catccgggtg atcacctcga
     5341 cggggaacgg gcccgagaag tcctgcacga catcgaagtt gtcggggtcg accttggaca
     5401 ggtagtgctc gacgagttcg acgacggtgt ctcgctgcga ctggatggcc cgcggcgtga
     5461 acgccttgtt gagcaggctg cgcatgtggc gatggtccgg cgggtccatg aagatgatcg
     5521 acttctgcgg gacctcgccg gtgcgcacca tgccgagatc gcaaccgcgc gacgacgaaa
     5581 acgactcgta gtccttcagt gccgccgcca catccgcgtg ccgcgtcagg gcgtagaagt
     5641 cctcgtcctc gtcgtaatag atcggtgcct cgtcccgcat ccgcttgtag atctcatacg
     5701 ggttgtcgaa gtactcctgt gcgaccggat tgaacttcag tttcggcttg gtcatggact
     5761 cctccttcgc ggcgggctaa tggccggaag cgttacgaga tatgttctaa ctgtaacgct
     5821 aacagttccg ttgtccagat accttggatc cggcattaat ggcgtcgtcg aggaggccga
     5881 gatcgctgcc gagttcggct gcgatcttgc gcacgaccgc gacgtccttg ccgacgaact
     5941 cgccgaccat gtcgatgaac gcgtccaccg aaccgcgggc gccgacgatg ctcgccaccc
     6001 ggctcgccgc gctgccgtga ccggtcgcgt ccagcagtcg cttttcgtcg acgcccagat
     6061 gctttcccag ccgcacagct tcctgcaaca gcccgatctg cgcggcgaac aacgtgttgt
     6121 tgaccagctt gacggcttgg cctgcgccga cggggccgac gtgaaggatg ggatcgccgt
     6181 agccggccag cacgcgccgg acacgttcga caacgtcatc ggcgcccccg acgaacagcg
     6241 tcacttgccc ggcagcgatg tcgtgcgggc cgccgctgac cggagcgtcg acgacgccga
     6301 taccgtgggc cgccgcctcg ctcgcgatgg cctccgcggt gcggggactc ccggtggtgt
     6361 ggatgacgat cgaggcgccg gactgcatcg tcgacatcag cacgctgtcc aaacagacct
     6421 gctgaacctg cccgtcggtg aacacgcaga cgatgacgac atcggcctct gctccgatgt
     6481 ccgcgagatc ggtgaccggc cgcgcgccga gttcactgac ggcgcggcgc ttctcgacag
     6541 atctgccgag cgcgcgcacc tcgtggccag ccccgacgag acgccgcacc atgggcgcac
     6601 ccatgcggcc cgccccgaca aaaccgaccc gactcaacgc gggtgatcca tcgacttcaa
     6661 ggcggcgtcg gccgcgtcga acaccgcacc ctctggcgcg gacgcaaccg aggcgaggct
     6721 ggcggcgtgg cggacatcct tctgcaacag gccgccggcg atcggtgcga gcccgtccag
     6781 cgttccgccg aataccgcga tgctgccgag cgccttgctg acggcggatc cgcgattgag
     6841 cacctcgcac agcttgtcgc gcggaatgcc gagggcatcg cccaactcga gcgtggtgat
     6901 cgcgctgccg agattcgcgg tgaacaacag attgttgagg atcttggtga cctggccgct
     6961 gccgagcgga ccgaggtgca cgatcgggtc ggcgtaggtg gcgaacaccg gccgacagcg
     7021 ttcgacgacg tcctcgtctc cgccgatcat gaccagcagt ttgccttcct caaccgccgg
     7081 cccgccgccg ctgaccggag cgtcgatcac cgaaactcct tgcgcagccg ccttttcggc
     7141 gatctccttg caggtatccg ggtgcaccgt gctgtgaacc gcgacggttc cgcctgaggc
     7201 caagccggcc aacactcctg tgtcgccgta caacacctcg cggacatcgt cgtcaccgac
     7261 gacgcacagg cagacaagat cgctggcggc ggcgagctcg gcgggggagc ccgcagtctt
     7321 cgcggcggtg tccgcgtacg gttcgaggct cgcctctcgg cgcgcccaga gcgtggtctc
     7381 gaagccgccc tcgacgatcc gtcgcgccat ggggccgccc tggctgccca gtccgatgaa
     7441 tccgacgcgc atcagcccgc ctcccgttct ttcgatgtgc cctgttgcgc ggcaacgcat
     7501 tccccgacga acgacagcac ctcggcgtgg tacgcggcgg cgctgagcga gagcgacaga
     7561 ttgtgaccgg tgccggcctg ctcgttgacc gtgaatcgcg gcgacaagct gaacatggcc
     7621 tcgatgtgct tgagcgtctc ggcatccgac cgccacaccc gctcgtgttc ggcgacggtg
     7681 aactgcactg gcacggctat gcgcgccgcg agctcgggaa agtactgtcg tggccagcgt
     7741 tgcgtcatgt cgacctcgta gagggcgccg gacgtcgagt tcgtcatgcc ggacaacacg
     7801 tcgtccggat acaaatgcga gggctgccac aggatgtcgc gcagtccctt ggggcgccgt
     7861 gttgcggtcg cggtctttag gatctccgcc gtatccgggt ggtattccac accggtgcct
     7921 gccaactcga gtccgatgac accagcgtcc ttggctctgt catcactggc cagccgcacc
     7981 gcgagttcgc agccggccga atggccgagc agaacaagtc cggctccgcg aggattgccg
     8041 ccgaggaccg tgtcgatcgc cccgtatgtc agcgccaccc gttgctccgg atgtgccatg
     8101 gcatcggggt agggtgccga cgcaccgtag cccggccggt cgagcgccac catcgtgtac
     8161 ccgttggcgg cgccggtgcg caacagcgag aagtccggat ggcccgggca gtcgaaatag
     8221 gctgcggtgc ttgcccctcc gtggaaggcg acgacgacgg ccttcggctc gtcggcgagt
     8281 gcgaccaggc ccgacatcgg cacgccgtcg acgaggacga cgcgcgggcg cgggctcatg
     8341 tgtcggtcct caaaaggatc gctccgctcg gggtgaggcc gccgctgctg accaccgcga
     8401 cgcgggcgtc cttcacctgg cgctcacccg cgtcaccgcg gagttgggtg acggcctcgt
     8461 ggatcaggcc catgccgtgg gtgcggccgt gggagagctg accgccgtgg gtgttgagcg
     8521 gtatcacgcc gtcgcgggcg atcgccttgc cgccgtcgag gaagtccttg gcctcgccga
     8581 tgccgcagaa tcccagcgcc tccagccagg acaggcagtt gaacgtgaac ccgtcgtaga
     8641 gctcggcaac gtcgacatcc ttgggctgca acgaggttcg cgtccatagg tgggcagcct
     8701 gccccagcac ctgtggttcg tgcgtcagcg tggtctggtc ccagtcggtg cgttcgacga
     8761 tctgggtgcc gacggcctcg aacagcaccg gtttgcgcgg catgtccttc gcgacatcga
     8821 ccgccgacac gaccacagcg atcgcaccgt cacacggcac gtcgcagtcg tacagcccaa
     8881 acggcgtcgt gatcgggcgt gcgttgaggt agtcggccat ggtcatcggg tcgcggtaga
     8941 tcgccgtcgg gttgagcgcg gcattcgcgc gctggttcaa cgcgatccag cccagggtct
     9001 cgcgcgtcgt gccgtaccgg gcgaagtggc gttgtgcgtt gagtgccaac gtatgagccg
     9061 ccgacgtggc gccgaacggc atctgccagc tggaggtgcg ggcgccccct ggcggggatg
     9121 tcctgccttc cttcatcagc tgctggaagg tcgcctccca cagcgtgcgg aagcacagca
     9181 cgtggcgggc catccccgtg gcgatcgcca tcacggccgc gatcaccgag ccgccgggac
     9241 cgaaggtgtc catgcctccg ttgatccatg tcggacgcag cccgagcgcg ccttccaagg
     9301 cgctgacccc gccttcgccc atgcctgcga tgtcgagtcc cggatacgtg gacagcccgt
     9361 cgatgtcgtc gaaggtcagc cccgcgtcgg caacggcttg ttcacaagcg tcgatggtca
     9421 acgacagtgg cggtagcatc tgccgacggc cgatcttcga catgccgatg ccggtgatgg
     9481 ccgcggtgtc ttcgaacttc tccttggtga gcatcggcgc gacgtgcttg ccgaagtcct
     9541 gaggcgcgat ttcgtcgaca gcctgcggtc ccgcctcagt ctcggacgac ggccggaaca
     9601 acggcagcca gacatcttcg atcttctcga acgtgacctc gaccgcctga ccaatcgtca
     9661 gatcgtcgtg ctcacactca acgatgttgg tggtcaatcg aactcgcgga tcctcgacga
     9721 tcgcgacttg ggccaccacg tacggaggcg ggaggtcggg gaatccgaac cggtggttga
     9781 ccgtgaaggc cgaaatcgta gccttgccgg agacatcgcg cacgcccatg ttgcggctgc
     9841 gacagtagcg gcacaccggc tgcggtggat ggatgagcgc ctcgcagtcg cggcactcct
     9901 ggatgcgcag cacgccgtcg ccgcccgcag tccagaagaa ctcgttctga agcgtgagtt
     9961 ccggcagtgg ccgtgtcacc ggacaaactc cgaaatggct tcgccgtctt tcggttcggg
    10021 ctccggcgat tcctgtgcgt cgtggtggac ggcccggtcg ccggttggcc ggtgttcgcc
    10081 catgtaaatg atcgcgtgca ccgggcagtc gaacagtgcg cgcatcaccg catcacggtc
    10141 cttctcgggc accgtgccgt cgccgatcag tgaggcgtag ccccagtcgt cgagcgaaaa
    10201 atactccggc gcgtgtttgg cgcagatgcc gaaaccgtca cagagtgtgc gatcgagccg
    10261 aatccgtaag ccgtcactca tgagtggccc accgcctcca cttcgtaggg ccgcagggca
    10321 tcgaatgcgg cagtccggca ggacgggcag tcgttggcga gatgttgagc gaccagttgc
    10381 gggaactgcc gcagcagact cgccgcgaca ttggtggcgc cgtcgagcgt gccgcatgcg
    10441 ccgcggcccc ggagcacgac cgaccatcgc tcgagacgcg ccaggtcctc atccgttgcc
    10501 acgccgtcgc gtaatgcgga cgcgacggcg gccattgcgg ccgtgccgtt gaagcatgat
    10561 ccgcactgac cggcattctc gcggtcgaag taggacagca ccgaggcggc gacggcgacg
    10621 gggcagtcgt cggtcagcag cgagatcgca ccgcagccaa ggccactgcc gaggcggcgg
    10681 atcgtctcgt ggtcgagggt cgcgtcgagg atgtcggtgt tgaccaggcc ggcgaaatag
    10741 ccacccatca gcgcaccacg caccgattcg gccgacagcc catgcaccga cagcagctcg
    10801 gaaaatgctg tgccatgagg gatttcgtat agtgccgcgg gccgacccgc gcctgtaatg
    10861 gtggccagga aggtacccgg cgacatcggc gtcccgacgg cacggaacgc ctgcgcgccg
    10921 tgcgcgtgaa tgaagggcag gttggccagc gtctcgacat tgctgaccat cgtcggcagc
    10981 ccgaacacgc cctcctcgaa cgggcggggc ggcttgtcgg tcggtttcgc gggtccgccg
    11041 ttgattcgcc ggacggtggc ggtctcctcg cccgcgacgt acgtcggttc gacggtgacc
    11101 acgctgacgg cggtgtcgcc gaacgtctct ggcttcaatt cctccagcgc cgatgcgacc
    11161 gccgtggccg attgttggtc ggagacgtag acgtatgcac gggtggcgcc gatgatcgcg
    11221 gtggccaacc gcagtccgtc gagcaccaca tgcggacgat tacgcagcag ccaccgatcc
    11281 ttgacggacg ccggctcgcc ctcttcgccg ttcgcaacca ccacggtttc ggacccccgg
    11341 cgtccggcat cgcggaccgt gcgcagcttg gtgcccagcg ggaaggcggc gccgccgcgg
    11401 ccgagcagcc ccgacaggtc gacctgctcc agcagcgcct cggcatcgtc cagcggcagg
    11461 taatcactgc cggccgacgg ccgcagcagt cgcggctcgc agttcggcca cgcagcaacg
    11521 atcagttcgg ttgcagtgga ggtcatgtga ggtccttccg gccggatagg ctgacggaca
    11581 tgaggactgc ggtggtgcgc gtcggtgtcg accgggccgg cgagctcgcg ccagcgcagt
    11641 tacgcgacgg catggcgcgc ctgggccagt tggccggacc agcgggcttc gaggtggtgg
    11701 acaacgatct cgtcggcctc cctccgcagc gccgtgaggt ggagctgctg atcgccggcg
    11761 acgacccagc cgacctcgag cggcgcgctg tcgagttgtg cgccaaagca ttcggcacca
    11821 caccggtgcc aggagtgctc acgttcatca gccggggcac cgacgcagac gcgcacggcg
    11881 tgctggcggg cttggggctg tccggcgaga tcgaacgcgt ggccggcgac gagggctggg
    11941 atgtggtgca cgtgacgctg cgcaaggcgg acctggaacg cgtcccggag agccgtgtcc
    12001 ataccgcgct cgaagcgtcg ctcaactgcg aggtacacat tcgcactatc tagccgtcca
    12061 ggaacttcag gatcgcgggc gccgcctgca cccacgtgtc gaacatgttg aagtgcttga
    12121 ccttcccctg ggcgttgtcc ttcgccgcct gctcccaggc gtcttccggc cagggcgggt
    12181 cgattagttc ggatcccttg atcaggcagc tcacctcgag cgacgtgcgc ttcgggtgat
    12241 cccagtcgtc ctcgccgccg cggatgatca gtgtgggcac cgtgatgttg tcgaacatct
    12301 cgtcttcgac accgggaatc gtttggccag gcttggacac gaatgcgttc agccagcgca
    12361 tcatcacctt gacgaattcg tcgggatcga gcgccagaag ccgcgcttcg ttggcgggat
    12421 tgtcggcgat gcgctccttc cactccctca ggtgcagaag gccttccatc cccatggtga
    12481 ggaccggccg aatgtttggc gtgatgtagt gcgccccgag cacgaaggtg ccgtacacac
    12541 cgccgacgat gttccacacc acgagctttg tcgctatctc ggggtacagc atgatcgtca
    12601 gcatggaatc cctggcgccg cccgaaccgc ctgcgatgat gcaagggccg atgcccagag
    12661 cggtgaccag agcgtgcagt gtttctgccc gcatgtgcga ttcggattgc ccgtagaact
    12721 gcacatcgga cttgccgcag tt
//
LOCUS       DASRJA010000022         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1953943,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000022 DASRJA010000000
VERSION     DASRJA010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953943"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFC01_01030"
     CDS             complement(<1..198)
                     /locus_tag="VFC01_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HZN78260.1"
                     /translation="MKASDVKRRWARWRDRLRDRPKAEFSYRIAVAVIGLAILGVGIL
                     AIPYPGPGWAIVFVGLAILATE"
     gene            complement(195..821)
                     /locus_tag="VFC01_01035"
     CDS             complement(195..821)
                     /locus_tag="VFC01_01035"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN78261.1"
                     /translation="MTTEFAGEHRGGDFSPIEPTSRGGPDYGRFVEAVRTVQDRARAA
                     DAPDAVITEAADLLESISNLLAPYEADEWSSPAGRRMDLPNRGSVLGIPAEYHKTDDD
                     RIGGIVHFRRFHLGRNGAVHGGCIAQLFDSLLGYTAFRLTRSLYQRTAFLHVDYRKIV
                     PIETKLQVDAGVDHVDGRKMFIAGRLLDGDDVLAEANALFVKLKPGQP"
     gene            complement(826..1479)
                     /locus_tag="VFC01_01040"
     CDS             complement(826..1479)
                     /locus_tag="VFC01_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011211011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN78262.1"
                     /translation="METRSDLLTGPSPGSDGVPERADAARNRARVLAAAADLFATRDP
                     RTVTMDDIAKAAGVGRATVYRRYPDTWSIAVALLDEHERMLQEKMLRGDPPLGPDAPP
                     AIRLAAFYAAMIELLDAHAELVLGTETGGARFATGAYGFWSTHVRSLLVDAGTPNPDA
                     LIDILLAPVSAEVYVHQRARGLTSVQITDALTRVAHGVLGDTRSRRPVRRPAQHPIP"
     gene            1655..2146
                     /locus_tag="VFC01_01045"
     CDS             1655..2146
                     /locus_tag="VFC01_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN78263.1"
                     /translation="MAERIKGKTLIARDEGDDASGVIIKLYSEETNGAISIIEQPFEP
                     GLLLPPHVHQNDVWLYILEGEMHARVADEVVRATPGCWVMKPRGVPHTMWNAGPQPAR
                     LIEVYTPGGFELFFKDFGDRLRQGPVGLDELNQLGEPHGIQFFDDWIAELTSAYNLRV
                     IGE"
     gene            complement(2153..3022)
                     /locus_tag="VFC01_01050"
     CDS             complement(2153..3022)
                     /locus_tag="VFC01_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN78264.1"
                     /translation="MTTQSVAQASGTFSIGGDLTVNRLGFGAMRLTGKGVWGPPADRE
                     ECIRVLRRAVELGVNFIDTANSYGPYVSEELIREALHPYDGVVIATKAGLLRTGPDVW
                     PVLGYPAYLRQECEMSLRRLGVDTIDLFQLHRIDDKFPAEDQIGELVKLQQEGKIRHI
                     GLSEVDTDQLEAAQKVATIVSVQNMYNITVRTAEPVLEACEAQGIAFIPWFPLAAGPL
                     AAPGGPLQRIADDHHATPSQLALAWLLKRSPVMLPIPGTSKVAHLEENVAAAEIELSD
                     EEFDTLSKAGSAG"
     gene            3060..>3478
                     /locus_tag="VFC01_01055"
     CDS             3060..>3478
                     /locus_tag="VFC01_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HZN78265.1"
                     /translation="MKLSDLAALPLTYSEVGATAGPLPAGYHHIQKAAVIGRGRRRFD
                     EAADKGMRWGMLRGAGVGVEPTTEVAAVGSEVIVHLGPVRAPCRVVYVVDEPDRRGFA
                     YGTLPGHAESGEERFVVAYDPATDEVHAEVTAFSRHGT"
BASE COUNT          592 a         1089 c         1200 g          597 t
ORIGIN      
        1 ttcggtggcc aggatcgcca gcccaacgaa gacgatcgcc caaccggggc cgggatacgg
       61 gatagcgagg atgccgacac cgaggatcgc cagcccgatc accgcgacgg cgatgcggta
      121 ggagaattcg gccttcgggc gatcccgcag ccgatcgcgc caccgcgccc aacgccgttt
      181 gacgtccgac gctttcacgg ctggcccggc ttcagcttga cgaacagcgc gttggcttcg
      241 gccagtacgt cgtcaccgtc gaggagccgt cccgcgatga acatcttgcg gccgtcgacg
      301 tggtccacac cggcgtcgac ctgcagtttc gtctcgatgg gcacgatctt tcggtagtcg
      361 acgtgcaaga acgcggtgcg ctgatagagg ctgcgggtga gcctgaacgc ggtgtagccg
      421 agcagtgagt cgaagagttg cgcgatgcag ccgccgtgca ccgcgccgtt gcggccgagg
      481 tggaagcgac gaaaatgcac gatgccgccg atacggtcgt cgtcggtctt gtggtactcc
      541 gccgggatgc cgaggacgct gccccggttc gggaggtcca tgcgccggcc ggccggcgac
      601 gaccactcgt cggcctcata gggtgcgagc aggttcgaga tggactcgag gaggtcggcg
      661 gcctcggtga tcaccgcgtc gggtgcatcg gccgcgcggg cacggtcctg cacggtgcgg
      721 acggcttcga cgaatctgcc gtagtcgggt ccgccgcggc tggtgggctc gatcgggctg
      781 aaatccccgc cgcggtgctc ccctgcgaat tcggtggtca cctcatcaag gtattgggtg
      841 ttgcgccgga cgccgtactg ggcgtcgcga acgggtgtcg ccgagcaccc catgggcgac
      901 gcgggtgagc gcgtctgtga tctgcaccga cgtcaagccg cgtgcccgct ggtgcacata
      961 gacttcggcg gaaaccggtg cgagcaggat gtcgatcaat gcgtccggat ttggcgtacc
     1021 agcgtcgaca agaagcgaac gcacgtgtgt cgaccagaat ccgtacgccc cggtcgcgaa
     1081 tcgagctccg cccgtctcgg tgcccagtac caactctgca tgggcatcga gcagttcaat
     1141 catcgcggcg tagaaagcag cgagccggat ggccggtggc gcgtccgggc ccaacggcgg
     1201 gtcgccgcgc agcatcttct cctgcaacat tcgttcgtgt tcgtcgagca gcgctacggc
     1261 gatcgaccag gtgtcgggat atcgccgata caccgtcgcc cgcccgaccc cggccgcctt
     1321 ggcgatgtcg tccatcgtca ccgtgcgcgg gtcacgagtg gcgaagaggt ccgcggccgc
     1381 ggccagcacg cgggccctgt tccgcgccgc gtccgcccgc tcaggtactc cgtcgctgcc
     1441 tggagatggc ccggtgagca ggtcggagcg ggtttccatg ggggcaatat agccgccaga
     1501 cggaataagt gggcacagtg tccagttagt gttatcgtgc caataaccgg acacagtgcc
     1561 cggttggccg cccggaacgt gactcggctc ttagcaagga gtaccgaaat gaactggccc
     1621 gcattgattt tcgcgcccac caggcaggca agccgtggct gaacgcatca agggcaagac
     1681 actgatcgcc cgcgacgaag gcgatgacgc aagcggcgtc atcatcaagc tgtacagcga
     1741 ggagacgaac ggcgccatct cgatcatcga gcagccattc gagcccggtc ttctgttacc
     1801 cccacacgtt catcagaacg acgtctggct ttacatcctt gagggagaga tgcacgcgcg
     1861 ggtcgccgac gaggtcgtca gggcaacacc cggatgctgg gtgatgaagc ctcgcggtgt
     1921 cccccacacc atgtggaacg cgggcccgca accggcacgc ctcatcgagg tgtacacgcc
     1981 gggtggattc gagctcttct tcaaggactt cggagaccgg ttgcggcagg gcccggtcgg
     2041 actcgacgaa ctgaatcagc tcggcgaacc gcacggcatc cagttcttcg acgactggat
     2101 cgccgagctc acatcggcct acaacctacg tgtcatcggc gagtagctga cctcagccgg
     2161 ccgacccggc tttgctcagc gtgtcgaact cctcgtcgga caattcgatc tcggccgcgg
     2221 cgacgttctc ctccagatgc gcgaccttcg acgtgccggg gatcggcagc atcaccggcg
     2281 agcgcttgag cagccaggcc agcgccagct gcgacggggt ggcgtggtga tcgtcagcga
     2341 tgcgctgcag cgggccacca ggcgcggcta gtggaccggc cgccagcggg aaccacggga
     2401 tgaaggcaat gccctgcgct tcgcacgcct ccaggacggg ctcggcggtg cggacggtga
     2461 tgttgtacat gttctgcacc gaaacgatgg tcgcgacctt ctgggcggcc tccaactggt
     2521 cggtgtctac ctccgaaagg ccgatatgtc ggatcttgcc ctcctgctgc agcttcacga
     2581 gttcaccgat ctgatcctcg gcggggaact tgtcgtcgat ccggtgcagc tggaacaggt
     2641 cgatggtgtc gacgccgaga cggcgcaggc tcatctcgca ttcctgacgt agataggccg
     2701 ggtagccaag aaccggccac acgtcgggcc cggtgcgtaa gagtccggcc ttcgttgcga
     2761 tgacgacacc gtcgtagggg tgcagcgcct cgcggatcag ttcctcggat acgtatgggc
     2821 cgtaagagtt ggcggtgtcg atgaagttga cgccgagttc gacggcgcgg cgcaggacgc
     2881 gaatgcactc ctcccggtcg gcgggcggac cccacacgcc ctttccggtc aggcgcatgg
     2941 cgccgaatcc caagcggttg accgtgaggt cgccgccgat gctgaatgtt cccgatgctt
     3001 gcgcgacgct ttgggtggtc atttcaccga ccgtacgcgg caatgccagg gtggttcctg
     3061 tgaagttgag cgacctcgct gcgctgccgt tgacgtattc cgaagtgggt gcgacggcgg
     3121 gaccgttgcc cgccggatac caccacatac agaaggcagc ggtcattgga cggggacggc
     3181 gccggttcga cgaagccgcc gacaagggca tgcggtgggg catgctgcgt ggtgccggtg
     3241 taggggtgga gccgacgacc gaggtcgccg ctgtggggtc ggaggtgatc gtgcacctgg
     3301 gcccggtgcg ggcgccgtgc cgcgtggtct atgtcgtcga cgaacccgac cggcgggggt
     3361 tcgcctacgg gacattgccg gggcacgccg aatcgggtga ggagcggttt gtcgtggcct
     3421 acgacccggc gaccgacgag gtgcacgcgg aggtgacggc tttctcacgg catgggac
//
LOCUS       DASRJA010000023        10541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1950963,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000023 DASRJA010000000
VERSION     DASRJA010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10541
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950963"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VFC01_01060"
     CDS             <1..751
                     /locus_tag="VFC01_01060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZN78266.1"
                     /translation="IFSKMLSGEIDLDEADPSFNRQRMAEGMAVRTRFFDNFFGAATD
                     AGIRQAVILASGLDTRAYRLPWPSGTTVYEIDQPEVIDFKTTTLADLGAQPATDLRVV
                     GIDLRNDWPAALRGKGFDLAQPTAWIAEGLLIYLPPDAQDQLFDNIGSLSAPGSRLAT
                     EYVPSTDIFTDERAQARSERWRRLGMNVDMRELVYDGKRSHVIRYLEAGGWHVTADDV
                     PDLYTANGFTFPDDEDAQAMADAKYITATLK"
     gene            757..1656
                     /locus_tag="VFC01_01065"
     CDS             757..1656
                     /locus_tag="VFC01_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN78267.1"
                     /translation="MARTEGDSWDLASSVGATATMVAAQRALSNREGLIDDPFAEPLV
                     RAVGLDFFVRALDGEIELEDVDPEFNMRRAAEGMTVRTRWFDTLFTDAATAGVRQAVI
                     LAAGLDARAFRLPWPDGTTVYELDQPEVIDFKTKTLIDLGAQPKADRRTIAIDLRNDW
                     PKALLDNGFDVSQPTAWIAEGLLIYLPPEAQDLLFDRINELSAPGSRVATEHIPDVTM
                     FSDERSQRIADRLKKYGHDIEMGDLIYHGERSHVIEYLTGHGWDVTSQTMREAYAASG
                     FEFPENETIGFFSNLSYVSAVKR"
     gene            complement(1628..2974)
                     /locus_tag="VFC01_01070"
     CDS             complement(1628..2974)
                     /locus_tag="VFC01_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HZN78268.1"
                     /translation="MSAKGVTIGIDIGTTAVKAVAADADGHVVARTRIPHQLRVPAPD
                     RLEHDADEAWRRGPLAALAELGRPDAKAVAVTAMVPSLTAVDDDGRPLTPGLLYGDSR
                     GRRAAGPDTTFVSGETVEFLRWTAREAPNAKGYWPAAAVANHALAGEAVVDSATASTT
                     YPLFDGTGWSEDACADCGATADRMPRVEATGTTVGQLRDADAVLAAGSVDALCEQLVA
                     DADRDGDVVVLCGTTLIVWVTIPEYREAPGLWTLPHSTAGKFQIGGPSNAGGLFLGWV
                     NQLVAEDDSTVDPGRVPVWSPYVRGERVPYHDPDRRAVLDGLDLTHGGGAVRRAAYEA
                     SGFVVRQFIDLSGASAARIVATGGGTRVQPWMQALADAADLPVEVSAVPEGAALGAAF
                     LARMAAGLETSTADAARWASTERVVEPDPVWADAMHDRYARFLELAEASASPRSRN"
     gene            3005..3982
                     /locus_tag="VFC01_01075"
     CDS             3005..3982
                     /locus_tag="VFC01_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN78269.1"
                     /translation="MTRLRALVTAPLRGDGFDKLRELADVVYDPWIDQRPLRIYTPEQ
                     LAERASAEDANVLVVESDSVSGPVFDLPLRAIASTRGDPNNVDVAGATAVGIPVLYTP
                     GRNAEAVAEMALALLFAATRHVITADADVRAGQAFRDGTIPYQRFRAWEVAGQTAGLV
                     GLGAVARALKWRLEGLGVKVIAYDPYNSEARHSLEELLAEADIVSLHAPVTDETAGMI
                     GAEQFGAMRDGVVFLNTARAQLHDTDALVDALRSGKVAAAGLDHFVGEWLPTDHPLAS
                     MGNVVLTPHIGGATWNTEARQAQMVADGVEALLAGRTPAHIVNPEVLAR"
     gene            3979..4635
                     /locus_tag="VFC01_01080"
     CDS             3979..4635
                     /locus_tag="VFC01_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HZN78270.1"
                     /translation="MRFVDNAEEAVLAAAKDMLRRGLVEGTAGNISARREDGNLVITP
                     SSVDYAEMALDDLVVVDPEGAVVQAKDGRSPSSEMKLHLACYQAFDDIGSVIHSHPVW
                     ATMFAVAHQPIPAAVDEFAVYCGGEIRCAEYAASGSPDVGTNAVKALEGRAAALIANH
                     GLVAVGPRPDKVLHVTALVERTAQIAWGARALGGPVPIPDEVNQNFAAVYGYLRQNPM
                     "
     gene            complement(4711..5298)
                     /locus_tag="VFC01_01085"
     CDS             complement(4711..5298)
                     /locus_tag="VFC01_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN78271.1"
                     /translation="MDFTETVIASPGRGAEKRIMAAATDLFYREGIHATGVERIAETA
                     RVSKRTLYRYFPTKNDLVEQYLQRIHDSGGIPNEQALDTPGATARNQLLAIFDSRPVD
                     RFRGCPFHDAAVEAADDMPGVHDIVHEHKLWFIARLTDKAAEAGAADPYQLGHQLAVL
                     FEGALALATSLDDTAAMIHARSASEILIDAAVRSA"
     gene            5435..6334
                     /locus_tag="VFC01_01090"
     CDS             5435..6334
                     /locus_tag="VFC01_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HZN78272.1"
                     /translation="MADSKELFLITGATGNTGAHTVRLLRERGHRVRAFVHTLDHRAA
                     RLSELGAEVVQGDLLDFPSLSAATAGVTAAYLNYPVAPGLVEATANFVQAASEAGVHA
                     VVNMSQISARREAKSNAARHHWLAERLLDRSALVTTHLRPTFFTFFLNMFWIRDNDEG
                     IYRLPFADGRHAPIDAADQAHVIAAILQNPDPHDRQVYPLFGAEELDWHEIAAKVQAT
                     LGIPVRYEPIDIPSFATGLTAAGASEHFVQHISSVAQDYRDGVFAGVNNLVEVIGGVK
                     PITVEDYVAATRSRFDTNGRPRR"
     gene            complement(6344..7015)
                     /locus_tag="VFC01_01095"
     CDS             complement(6344..7015)
                     /locus_tag="VFC01_01095"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN78273.1"
                     /translation="MEIMTEHRPHTATKHDYVPAFGKDALLPFYDLLTRVLGMGRVYD
                     ELVAQADLGDGLRVLEIGCGTGNVTLRAKRAAPGADVIGTDPDPLALARAQRKARGMT
                     GIRFERAYAQELPFADGEFDRALSSMMLHHLDEDVKAGAVAELHRVLHPGGQLHIVDI
                     GGHMTAHHGLAARWMKDNPHAAGNLGDAIPRLLRSVGFDCEEVGTRRQRFIGQVTFYR
                     ATRPA"
     gene            7081..7929
                     /locus_tag="VFC01_01100"
     CDS             7081..7929
                     /locus_tag="VFC01_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZN78274.1"
                     /translation="MTQASRLVRHRGGMPVYQYRADSDTPPVSVIRAHGILEHRVHIH
                     DFPVLWYAHTAGLVYVVAPGAAVDPAKVPHRDNGVGLFFDPAALDGDGRSPWPSWRAH
                     PLLFPFVHGQSSGLLRLTVPAARQPVWDTAIDSIEAELAARKEGYRQAALAHLTLLLI
                     DVARLAGDVVGDLRRSGEPLLADVFTVIDRHRGEPLSLRDVAHELGMTPGHLTTVVRR
                     RTGRTVQEWIIERRMAEARSLLSETELPVNEIAHRVGMSDPGYFSRQFRRIHGTSPRE
                     WRRRPA"
     gene            complement(7937..>10541)
                     /locus_tag="VFC01_01105"
     CDS             complement(7937..>10541)
                     /locus_tag="VFC01_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZN78275.1"
                     /translation="DNRLVTLTGAGGAGKTRLAIEISAHVASQFPSGVWYVDLAPTTY
                     AEVVPVVVARALGLPDQPGTSTPDTLVRFVADRETLLVLDNCEHLIDATASLTTNLLG
                     ACPALRVLTTSREPIGVAGEVTFLVPSLSLANEAVELFADRARRVRPDFSVTDDNAAT
                     VTEICRRLDGMPLAIELAAARVRALSLDEILGSLHDRFRLLTGGARTAVRRQQTLRAS
                     VDWSHALLTETERILFRRLAVFLGGFDLDAAQAVAGDAEVERYQVLDQLALLVDKSLV
                     VAENTSGATRYRLLETVRQYAQEKLSESGEAATVRSRHRDHYTAMAALLDAPGRSDYE
                     QRVEQADESMDNLRSAFGWSAETGDIGRALELASSLQPLWLSRGRIKEGLAWFDAVLT
                     DEIEVQPAVRARALADKAVLDAWVNIYSLDASEQALTIARELDDPGLLVRALTACGSA
                     SVYDAEVASRYLAEASGLARELGDRWRLDYILGQQAQAAFVAGDPVAVRAAAEEGRDL
                     ADAIGDRFGSRQCRWRLAGAYIQEGDLAGAIAELRGVVAEAEAAHDVITRVIALLVLP
                     HALASYGDASGARASAEAGIESAANLGDVHMGGCYIALMLAHLAAGEVALAAEAAEAA
                     WSRVSDLYGTASINSAYVAETALARGDLAAARRGADNAIAATSGWFLAAALKIRARIA
                     IAQGDTELCERDAHDALACCARVKGYLGVPDILEILGGLASDAGRHREGVRLFGAADG
                     IRQHTGEVRFPIYQADYEVLVAGLRNALGEADFEVGWGEGAALCTEEAIAYAQRGRAE
                     RKRPSSGWAALTPAELDVTRLVREGLANKDIAARLFVSPRTVETHLTHVYTKLGLSSR
                     VQLAQEAARHA"
BASE COUNT         1830 a         3560 c         3402 g         1749 t
ORIGIN      
        1 aatcttcagc aaaatgctga gtggcgagat cgacctcgac gaggccgacc cgtcgttcaa
       61 tcgtcagcgg atggccgagg gcatggcggt gcgcacccgg ttcttcgaca acttcttcgg
      121 tgcggcgacc gacgcgggta tccgccaagc cgtcatcctg gcgtcgggac tcgacacgcg
      181 cgcgtatcgc ctgccgtggc cgtcgggcac caccgtgtac gaaatcgatc agccagaggt
      241 catcgatttc aagacgacga cgctcgccga tctcggcgcg caaccggcca ccgacttgcg
      301 tgtcgtcggc atcgaccttc gcaacgattg gccggcggcc ctgcgcggca agggattcga
      361 ccttgcacaa ccgacagctt ggatcgccga aggtctgttg atctatctgc cgccggatgc
      421 ccaagaccag ttgttcgaca acatcgggtc gctcagcgcg cccggcagcc ggctggctac
      481 cgagtacgtg ccgagcactg acatcttcac cgacgagcgg gcacaggccc gcagcgagcg
      541 gtggcgccgc ctcggcatga atgtcgatat gagggaactc gtctacgacg gcaagcgcag
      601 ccacgtgatc cggtacctgg aggcgggcgg ctggcatgtc accgcggatg acgtgccaga
      661 tctctacacc gccaacggct ttacgttccc tgacgacgaa gatgctcaag cgatggccga
      721 cgccaagtac atcaccgcga ccctaaagta ggagacatgg cacggacaga aggtgattcc
      781 tgggacctgg cctccagtgt gggcgccacc gccacgatgg tggccgcaca gcgggcattg
      841 tccaaccggg agggcctgat cgacgacccg ttcgccgagc cgctggtgcg cgcggtcgga
      901 ctggacttct tcgtcagggc gctcgacggt gagatcgagc tggaggacgt cgaccccgag
      961 ttcaacatgc gacgggccgc ggagggcatg acggtccgca cccgctggtt cgacacgttg
     1021 ttcaccgacg cggccaccgc gggtgtccgc caggcggtga tccttgccgc tggtctggac
     1081 gcgcgggcgt tccggctgcc gtggcccgac ggaacgactg tgtacgagct cgaccagccc
     1141 gaggtcatcg acttcaagac gaagacgctg atcgacctcg gtgcgcagcc caaggccgac
     1201 cggcgcacca tcgcgatcga tcttcgcaac gactggccga aagcactgct ggacaacggc
     1261 ttcgacgtaa gccagccgac ggcgtggatc gcagagggct tgctgatcta cctaccgccg
     1321 gaagcgcaag acttgctgtt cgaccggatc aacgagctca gcgcgcccgg cagccgggtc
     1381 gcaactgaac acattcctga cgtgacgatg ttctccgatg agcgctcgca acgcatcgcc
     1441 gaccggctca agaagtatgg ccacgacatc gagatgggag acctcatcta tcacggcgag
     1501 cgcagccacg tcatcgagta cctgacgggg cacggctggg atgtgacatc gcagacgatg
     1561 cgggaggcat acgccgccag tggttttgag tttcccgaga atgagacgat cgggttcttc
     1621 tcgaatctca gttacgtgag cgcggtgaag cgctagcttc ggccaattcg aggaatcgcg
     1681 cgtagcggtc gtgcatcgcg tcggcccaca ccggatcggg ttcgacgacg cgttccgttg
     1741 atgcccaccg ggcggcgtcg gctgtcgacg tttcgaggcc cgccgccatc cgcgcgagga
     1801 acgccgctcc cagtgccgca ccctccggca cggccgatac ctcgacgggt aaatcggcgg
     1861 catcggccag cgcctgcatc cacggctgta cgcgggtgcc gccaccggtc gccacgatgc
     1921 gagcggccga tgctccgctg aggtcgatga attgacgcac gacgaagccc gacgcttcgt
     1981 aagcggcccg ccgtactgca ccgccaccgt gggtgaggtc cagaccgtcg agaaccgctc
     2041 gccgatcggg gtcgtggtag ggcacgcgct cgccgcgcac atacggtgac cacaccggca
     2101 ctctgcccgg gtcgactgtc gaatcatctt cggccacaag ctggttcacc cagccgagaa
     2161 acaggccgcc ggcgttgctc ggtccaccga tctgaaactt gcccgcggtc gagtgcggca
     2221 gcgtccacag gccgggcgcc tcgcggtact cgggaatcgt cacccatacg atcagcgtcg
     2281 tgccgcacag caccaccacg tcgccatccc gatcggcatc ggcgacaagc tgctcgcaca
     2341 acgcatcgac cgaacctgcg gccagcacag catcggcgtc gcgtagttgg cccaccgtcg
     2401 tgccggtcgc ttcgaccctg ggcatgcggt cggctgtcgc cccgcagtcg gcgcaggcgt
     2461 cctcgctcca gccagtcccg tcgaacaagg gatacgtcgt gctcgcggtg gcactgtcga
     2521 cgaccgcctc gcccgccaga gcgtggttgg cgacggccgc cgcgggccag tatcccttgg
     2581 cgttgggcgc ctcgcgcgcc gtccagcgca ggaattcgac cgtttcaccg ctgacgaacg
     2641 tggtgtcggg gccggccgct ctcctgcctc ggctgtcgcc atagagcagg ccaggtgtca
     2701 gcggcctgcc gtcgtcgtcg acggccgtca gggacggcac catcgctgtg acggcgacgg
     2761 ctttggcgtc gggcctgccg agttcggcca gcgccgccag cgggccacgc cgccaggcct
     2821 cgtcggcgtc gtgttcgaga cgatctgggg caggcacccg gagctggtgc ggaattcgtg
     2881 tcctggccac cacatggccg tccgcgtcgg cggccacggc tttgacggcg gtcgtgccga
     2941 tgtcgatgcc gattgtgacc cctttggcag acacgggcgt caccgtacgc cagcatgatc
     3001 tgtcgtgacg cgacttcggg ccctggtgac ggctcccttg cgcggcgatg gcttcgacaa
     3061 gctgcgggaa ctcgccgacg tggtctacga cccgtggatc gaccagaggc cgctgcggat
     3121 ctacacaccc gaacaactcg cggaacgcgc atcggcagag gacgcgaatg tgcttgtcgt
     3181 cgagagtgat tcggtcagtg gcccggtgtt cgacctgccg ctgcgggcca tcgcatcgac
     3241 gcgcggcgac ccgaacaacg tcgacgtcgc gggtgccaca gctgtgggca tcccggtgct
     3301 ctacaccccc gggcgtaatg ccgaggccgt cgcggagatg gcgcttgccc tgctgtttgc
     3361 ggccacccgt cacgtgatca ccgccgacgc cgatgtgcgg gcaggacagg cgtttcgcga
     3421 cgggacgatc ccctaccagc ggttccgggc gtgggaggtg gccgggcaga ccgcaggcct
     3481 cgtcgggctc ggcgccgtcg ccagggccct gaagtggcgc ctcgaagggc tcggcgtcaa
     3541 agtcatcgca tacgacccgt acaacagcga ggcgcgccac agcctcgagg agctgctcgc
     3601 cgaggccgac atcgtctcgc tgcacgcacc ggtcaccgac gagaccgcag gcatgatcgg
     3661 cgccgagcag ttcggggcga tgcgcgacgg cgtggtcttc ctcaacaccg ccagggccca
     3721 gctgcacgac accgacgcgc tggtcgacgc gctgcgcagc ggcaaggtag cggccgccgg
     3781 ccttgaccac ttcgtcggcg aatggctgcc aaccgatcac ccactggcga gcatgggcaa
     3841 cgtcgtcctg accccgcaca tcggcggggc gacgtggaac actgaggccc gacaggcgca
     3901 gatggtcgct gacggcgtcg aggcactcct ggccgggcgc acgcccgcgc acatcgtcaa
     3961 cccggaggtg ctggcccgat gagattcgtc gacaacgcgg aagaggccgt gctggccgcc
     4021 gcgaaggaca tgttgcgccg gggcctggtc gagggaaccg ccggcaatat ctcggcccgc
     4081 agggaggacg gcaatctcgt catcacgccg tcttcagtcg actatgcgga aatggcgctc
     4141 gacgacttgg tggtggtcga ccccgagggc gccgtggtgc aggcgaagga cggacggtcg
     4201 ccgtcgagcg agatgaagct tcacctggcc tgctatcagg ccttcgacga catcggcagc
     4261 gtgatccaca gccatccggt gtgggcgacc atgttcgccg tcgcgcacca gccgattccg
     4321 gcagccgtcg acgagttcgc ggtgtactgc ggcggggaga ttcgctgcgc cgagtacgcc
     4381 gcatccggtt caccggacgt cggcaccaac gcggtcaagg ctttggaggg tcgggcagct
     4441 gcgttgatcg ccaatcacgg cctcgtggcg gtcggcccgc gccccgacaa ggtcctgcat
     4501 gtgacggcgc tggtggagcg gacggcccag atcgcctggg gggcaagggc tctcggtggt
     4561 cccgtgccca tccccgacga ggtcaaccag aacttcgccg cggtgtacgg ctacctgcgc
     4621 caaaacccca tgtagcacac gccgcgagcg tgcatgtctg tacacgacac gccgccgaat
     4681 tccgtacaaa tgcgcacgtt cacgcggtag ttaggccgag cggactgccg cgtcgatgag
     4741 gatttcggat gctgaccggg cgtggatcat ggcggcggtg tcgtcgagcg aggtagccaa
     4801 cgccagcgca ccctcgaaga gcacagccag ctgatgaccc aattggtagg gatcggcggc
     4861 gcccgcttcc gcggctttgt cggtgagacg cgcgatgaac cacagcttgt gttcatgaac
     4921 gatgtcgtga acacctggca tgtcgtccgc tgcctcgaca gccgcgtcgt gaaaagggca
     4981 gccgcggaag cggtcgacgg gccggctgtc gaagatcgcc aatagctggt tgcgtgcggt
     5041 tgcacctggg gtgtcgagcg cctgctcgtt gggaatcccg ccgctgtcgt gaatgcgttg
     5101 caggtactgc tccaccaggt cgttcttggt tggaaagtac cggtagagcg tgcgcttgga
     5161 gacccgcgca gtctcggcga tgcgttccac acccgtcgcg tggatgccct cgcggtagaa
     5221 caggtccgtt gcggccgcca tgatccgctt ctcggcacca cggcctggcg acgcgatgac
     5281 ggtctccgtg aagtccatcg gccaaaagta caccgttcgg tgcgggcacg tccaaattgg
     5341 ctgttaattc cttctggctt ggaataccga tcggtttact tcggttgatc gaggcaccag
     5401 ctcaagacct ctgcgcgtga gaaaggcaat caatatggcc gacagcaagg agttgttcct
     5461 gatcacaggc gccaccggca acaccggtgc acacaccgtg cgcctgttgc gcgaacgcgg
     5521 tcatcgcgtg cgggctttcg tacatacgct tgatcaccgc gccgcgcgcc tgagtgaatt
     5581 gggcgccgaa gtggtgcaag gcgacctgct ggactttccg tcactcagcg cggcgacggc
     5641 aggagtgacg gcggcgtacc tcaactaccc cgtcgccccc ggtttggttg aggccaccgc
     5701 gaacttcgtg caggcggcca gtgaggccgg cgtgcacgcg gtggtgaaca tgtcgcagat
     5761 ctcggcgcgc cgggaggcta agagcaacgc tgcgcgccac cattggctcg ccgagcggct
     5821 gctggaccgc agcgcgctag tcaccacgca cctacggccc accttcttta cgtttttcct
     5881 gaacatgttc tggatccgcg acaacgacga agggatctac cggctgccct tcgcagacgg
     5941 ccgccacgcc cccatcgacg ccgccgatca agcccatgtc atcgcggcca tactgcagaa
     6001 ccccgatccg catgaccgcc aggtctaccc gctcttcggt gccgaagaac tcgactggca
     6061 tgaaatcgca gcgaaagtcc aagcgaccct gggcatcccg gtgcgctatg agccgattga
     6121 cattccgtcg ttcgcgacag gcctgaccgc cgccggcgca tcggagcatt tcgtgcaaca
     6181 catcagcagc gtggcacagg actaccgcga cggcgtcttc gccggggtta acaacctcgt
     6241 cgaggtgatc ggcggagtca agcccatcac ggtggaagac tacgtcgcgg ccacacgatc
     6301 ccggttcgac acgaacggtc gcccccgtcg gtgagctcag tcgtcaggcc gggcgggtcg
     6361 cgcggtagaa cgtgacctgt ccaatgaagc gctgtcgacg cgtgccgacc tcctcgcagt
     6421 cgaaccccac cgaccggagc aaccgcggga tcgcatcacc gagattgcct gcggcatggg
     6481 gattgtcctt catccaccgc gcggccagtc catggtgggc ggtcatgtgc ccaccgatgt
     6541 cgactatgtg caactggccg ccggggtgca ggacacggtg taactccgct accgcacccg
     6601 ccttcacgtc ttcgtccaaa tggtgcagca tcatcgacga cagcgcccga tcgaattcgc
     6661 cgtctgcgaa cggtagctcc tgcgcgtacg cgcgctcgaa gcgaatcccg gtcatgccac
     6721 gcgcttttcg ttgcgcacga gcgagcgcca gcgggtcggg gtccgtgccg atcacgtccg
     6781 cacccggcgc agcgcgcttg gcacggagcg tgacgttgcc cgtgccacag ccgatctcca
     6841 ggactcgcaa cccgtcaccg aggtcggcct gcgcgacgag ctcgtcgtag accctgccca
     6901 tgcccagcac gcgggtcagc aggtcgtaga acggaagcag cgcgtctttg ccgaaagcgg
     6961 ggacgtaatc gtgcttggta gcagtatgtg gacgatgttc ggtcatgatt tccatgattg
     7021 ccgtctaatg cacaagggtg ttggccgatc gtcggcaaaa gttggactat ctttggtcac
     7081 atgacccagg cttcgcgatt agtccgtcac cgcggaggca tgcccgtcta tcaataccgg
     7141 gccgattccg acacccctcc cgtttcggtc atccgcgcac acggcattct cgaacatcgc
     7201 gtgcacattc acgacttccc ggtcctgtgg tacgcccaca cggcgggcct ggtctacgtg
     7261 gtcgcgccgg gtgccgccgt cgacccggcg aaggttcccc accgagacaa cggggtcggc
     7321 ctgttcttcg atcccgccgc actcgatggg gacgggcgat cgccatggcc gtcgtggcgc
     7381 gctcacccgc tgctgttccc attcgttcat ggacagtcca gcgggctatt gcgactgacg
     7441 gtgcccgccg cgcgacagcc ggtgtgggac accgcgatcg actccattga agccgaactg
     7501 gccgcccgca aggagggcta tcgacaggcg gcgctggccc atctcacgct tctgctgatc
     7561 gacgtcgcac gactggccgg cgacgtggta ggtgatctca ggcgcagcgg cgaacccctt
     7621 ctggccgacg tgtttaccgt gatcgaccgc caccgcggcg agcctctttc actgcgcgat
     7681 gtggctcacg aactcggtat gacgccgggg catctgacca ctgtcgtgcg ccgtcgcacc
     7741 ggtcgcacgg tgcaggagtg gatcatcgaa cgtcgcatgg ccgaagctcg cagcctgctg
     7801 tccgagaccg agctgcccgt caacgagatc gcccaccgag tcggaatgtc cgatcccgga
     7861 tacttcagca ggcagttccg tcgcatccac ggcacatcgc cccgcgaatg gcgtcgtcgc
     7921 ccggcctagt cagcactcag gcgtgtcgtg ctgcctcttg ggcgagttgg acccgcgaag
     7981 agagaccgag cttggtgtag acgtgggtga ggtgggtctc gacggtacgc ggcgagacga
     8041 aaagccttgc cgcaatgtct ttgttggcta gaccttcacg aactagccgc gtgacatcga
     8101 gctcggccgg agtgagcgcg gcccagccgc tcgatggccg cttgcgttcg gctcgcccac
     8161 gctgtgcgta agcgatcgcc tcctcggtgc acaatgccgc accctcgccc cagccgacct
     8221 caaaatctgc ctcgcccaag gcattacgca gccctgcgac caagacttcg tagtcggctt
     8281 ggtagatcgg gaagcggacc tcgccggtgt gctggcggat tccatccgct gcgccgaaga
     8341 gccggacccc ttcgcggtgc ctgccggcat cactggcgag accaccgagg atttcaagga
     8401 tgtccggaac gccaagatac cctttgactc ttgcgcaaca ggcaagggcg tcatgtgcgt
     8461 cgcgctcgca cagttccgtg tcaccctgcg ctatcgcgat gcgggcacga attttcagcg
     8521 ccgccgccaa gaaccaccca cttgtcgccg caatggcatt gtcagcccca cgccgggccg
     8581 cggcgaggtc gccccgcgct agggcagttt cggcgacgta cgcgctatta atcgacgccg
     8641 tcccatacag atcactgacg cgcgaccagg cggcctccgc ggcctcggcc gccaacgcga
     8701 cctcacccgc ggccaggtgt gcgagcatca acgcgatgta acaaccgccc atgtggacat
     8761 caccgaggtt cgcggcggac tcgatgcccg cctcggccga tgcccgggct ccgctcgcgt
     8821 caccgtaact cgccagcgcg tggggcagca caaggagcgc gatgacccgc gtgatcacgt
     8881 cgtgagcggc ctcggcttcg gcgaccaccc cgcgcaattc ggcaattgcg ccggccagat
     8941 cgccctcttg tatgtatgca ccggcgagcc gccaccggca ctgccgcgat ccgaagcggt
     9001 caccgatggc gtcggcaagg tcgcgtcctt cttcggcggc cgcgcgcacc gcgactggat
     9061 cgccggcgac gaacgccgcc tgagcctgct ggccgaggat atagtccaac cgccacctgt
     9121 cccccagttc ccgggccaga ccactcgcct cggcgaggta tcgacttgcc acctcggcgt
     9181 cgtaaacgga cgcgctgccg caggcggtga gcgcccggac cagcagcccc ggatcgtcga
     9241 gctcacgcgc gatcgtcagc gcttgctcag aggcatccaa gctgtagatg ttgacccacg
     9301 cgtcgagcac agccttgtct gcgagcgccc gcgcacgcac ggcaggctgc acctcgatct
     9361 cgtcggtcag cacggcgtcg aaccaggcca gcccttcttt gatgcggcca cgggacagcc
     9421 agagcggctg cagagacgat gccagctcca acgcgcgtcc gatgtcgccg gtttcggcgc
     9481 tccacccgaa tgcgcttcgc aggttgtcca tcgactcatc tgcctgctcg actcgctgtt
     9541 cgtagtcact ccgtcccggc gcatcgagca aggcggccat cgcggtgtag tggtcgcggt
     9601 gacgtgaccg caccgtcgcg gcctcgccgg actcgctcag cttctcctgc gcgtattggc
     9661 gcacagtctc gagtaaccgg tatcgcgttg cgccactggt gttctcggcg accaccaatg
     9721 acttgtcaac cagcagggcg agctgatcaa gaacctggta gcgctcaacc tcggcatcac
     9781 cggcgacggc ttgggcggcg tcaagatcga acccacccag gaagacagcg agccggcgga
     9841 acaggatgcg ttccgtctcg gtcagcaatg catgcgacca gtccacggac gcgcgcaggg
     9901 tctgctgtcg gcgcaccgca gtgcgcgcgc cgccagtcaa caggcggaag cggtcatgca
     9961 gactgccgag gatctcgtcg agcgacaagg cccgcacccg tgccgccgcc agctcgatgg
    10021 cgagcggcat gccgtcgagc cggcggcaga tctcggtcac ggtagcggcg ttgtcatcgg
    10081 taacgctgaa atcgggccgc acccgccgag cccgatctgc gaacaactca acggcttcgt
    10141 tggcaagcga cagcgatggc actagaaacg tcacttcacc ggccacgcca ataggctcac
    10201 ggctcgtggt gagaacccgc aacgccggac aggcgcccaa gagattagtc gtcagggatg
    10261 cggtcgcgtc gatcaggtgc tcacagttgt ccagcacgag cagcgtctcc cgatcggcga
    10321 cgaaccgcac gagcgtgtcg ggcgtcgaag tgcccggttg atcgggcagg ccgagcgcac
    10381 gggccaccac gaccggcacg acttcggcat atgtcgtcgg cgccaagtcg acgtaccaga
    10441 cgccgctggg aaactgcgac gctacgtgag ccgagatctc aatggcgaga cgcgttttgc
    10501 ccgcaccccc ggcaccggtc aacgtcacga gccggttgtc g
//
LOCUS       DASRJA010000024        26796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3362064,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000024 DASRJA010000000
VERSION     DASRJA010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 26796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26796
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3362064"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VFC01_01110"
     CDS             <1..1209
                     /locus_tag="VFC01_01110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN78276.1"
                     /translation="AYDGSMAGPEDAELGLDLLTPRGTTYLATAPGDLGLARAYVSGD
                     VEPHGVHPGDPYELLKALAEKMDFKRPPARVLANIIRSIGFEHLKPIAPPPQEALPRW
                     RRMAEGLRHSRIRDAEAIHHHYDVSNTFYEWVLGPSMTYTCACYPHPDATLEEAQENK
                     YRLVFEKLRLRPGDRLLDVGCGWGGMVRYAARHGVKAIGVTLSKEQAEWAQNAIDEQG
                     LSDLGEVRHSDYRDVRESGFDAVSSIGLTEHIGVHNYPAYFRFLKSKMRSGALLLNHC
                     ITRHDNRTGASAGGFIDRYVFPDGELTGSGRIISEAQDVGLEVVHEENLRHHYALTLR
                     DWCRNLVEHWDEAVEEVGLPTAKVWGLYMAGSRVGFETNVVQLHQVLAVKLDSHGGDG
                     NLPLRPWWTP"
     gene            1454..1993
                     /locus_tag="VFC01_01115"
     CDS             1454..1993
                     /locus_tag="VFC01_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78277.1"
                     /translation="MLVGGTYRPYSPDAGHRVSALVFDDHAQIDNDANPFSARAVGAR
                     LMIFPICEVNRRTRRSRLLNKLDLLDRKFTDDVGRVLEAEPDLKAKVSRLVVDIAWVA
                     ARALSEEGKADASGTVVSEDLFNRARELGAEIQARTQNQELITAIDDALGSSEEVIGV
                     AVSELLAKLEHRATVRARS"
     gene            2010..4325
                     /locus_tag="VFC01_01120"
     CDS             2010..4325
                     /locus_tag="VFC01_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78278.1"
                     /translation="MGLIEIELSRTEFLSLATFLVKQSPIPDPTFEVLGNDRLVRSFD
                     CTNVTLGNAPPDLAPPPGVVAAKVDVKIGHASVAELEADANAKTVTQAVAWVYISLVP
                     IPGKLTFVPVGKGQHLLIADLIRIDVQGRPVTGRFDDPASPRWGRQPLGTLQIPLPDI
                     FSDIPITGSAVVLSAAAATLRVATAGQNVFLPAAERLVQFGDKWLLRVSPEVFTELLL
                     TALMDSVTPAPPGGATLIQPPSVTWGPTAIGWPGGPPWAVDLNGWGASGSYRLTKHKA
                     CGDEDVSLTVHTTLLPVPDAASSKLTFYLEISAQADDGTTFKCWLNNLGVVTLVAFFV
                     NPIIGIGVGLGSYAIVGGKVDDGVGEGVTGQSGGKGFKTIGSGDNFTIHSLEVDLNLG
                     ALAKAGATMQHGASAADGVTFGGELNLLPLSLIDHSCSFWPPNGATLKGTWSSGYNCN
                     QGWHASFELPRIHITDNVEVLNTEKLFGKEVAVYDTSWVVPSAHWTIDWPGPAVIDQY
                     VSVIGDLWAYTGPGLHVGSHFLPLPAGHVFLHTSAGIARFEIDHVPAPPAQPTPAELI
                     AMKANCMMLVERFEDVRWLVDPPDFDYGHDPLRHWTVYVEKAPGRMQLQIHGVQQGAP
                     TRRLASFSAESNETIAIEVITDGRTELRIEHDLDGSLEHTSIKQQWLLPTSTVDLNGK
                     ASTLELVGDRLEARPAPSVMSISTAAVAESRYADVPPASVALPDSRVAAVDGHRLVVG
                     YPFGGTAVHPPAPFRSSVETAPSVKGGCCGE"
     gene            complement(4335..6179)
                     /locus_tag="VFC01_01125"
     CDS             complement(4335..6179)
                     /locus_tag="VFC01_01125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunits gamma/tau"
                     /protein_id="HZN78279.1"
                     /translation="MALYRKYRPATFADVVGQEHVTEPLSTALAAGRINHAYLFSGPR
                     GCGKTSSARILARSLNCEQGPTPTPCGVCDSCVALAPNGPGNMDVTELDAASHNGVDD
                     ARELRDRAFYVPGESRYRIFIIDEAHMVTPQAFNALLKIVEEPPEHLIFVFATTEPEK
                     VLPTIRSRTHHYPFRLLAPRTMRSLLERICASEDVTVDDAVYPLVIRAGGGSPRDTLS
                     VLDQLLAGAEGNRVDYQRALSLLGATDVALIDVAVDALAAGDAAALFSAVEAVIDAGH
                     DPRRFATDLLERFRDLIVLQSVPDAIARGVVDGPEDVLERMRDQATRIGTATLTRYAE
                     VVHAGLGEMRGATAPRLLLEVVCARLLLPSASDTESALLQRVERIETRLDMSIPAGEG
                     AASAPDAPPKQYARKSRTEPTPKAVPKPEPAPQPKVEAEPAPPAPEPPPSPPPVRAPS
                     DPAVEAPAATVPSMGGEPNAAAVRSMWTTVREKVRRRSRTIDVMLAGAIVRAVEGDTL
                     VLSHESAPLAKRLVEQRNADVIREALKDALGVDWKIRCEVGTAAAAPPAPEPDDPVVS
                     APPQRDEDEEDMLAEAGNDSSEVQRRDPEEAALELLQNELGARRIDGG"
     gene            complement(6219..7502)
                     /locus_tag="VFC01_01130"
     CDS             complement(6219..7502)
                     /locus_tag="VFC01_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN78280.1"
                     /translation="MSFQSLGRDELQAQHEQQKRNYADLQAKGLNLDLTRGKPSPAQL
                     DLSNALLSLPGDGRDAFRDGDGTDTRNYGGLLGLPELRAIFSELLGIPVPNLIAGNNA
                     SLELMHDVVTYSMLHGGVDSTRPWIAEPVVKFLCPSPGYDRHFAITETLGVEMITVPM
                     REDGPDVDLIEELVASDPAIKGMWCVPVYSNPTGVTFSWDTVRRLVQMRTAATDFRLI
                     WDNAYPVHTLTHDFVQQVDVLGLAEAAGNPNRPLVFSSTSKITFAGAGVSFFGGSLGN
                     IAWYLQYAGKKSIGPDKVNQLRHLRFFGDADGVRLQMQRHQQLLAPKFALVQEILTDR
                     LGESKVASWTDPKGGYFVSLDVLPGTAKRTVALAKDAGIAVTEAGSAFPYRKDPEDKN
                     IRIAPSFPGLPELRDAIDGLATCALLSATESLLKG"
     gene            complement(7534..7628)
                     /gene="ffs"
                     /locus_tag="VFC01_01135"
     ncRNA           complement(7534..7628)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFC01_01135"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            7697..7782
                     /locus_tag="VFC01_01140"
     tRNA            7697..7782
                     /locus_tag="VFC01_01140"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7731..7733,aa:Ser,seq:gga)
     gene            7854..8606
                     /locus_tag="VFC01_01145"
     CDS             7854..8606
                     /locus_tag="VFC01_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78281.1"
                     /translation="MGRTVAVVWHASFSIAAGVGYFFFVLPRWPELLGDTSHTLGTVL
                     RIVAGALIGLAALPVVFTLLRTRRPEFGTPQMALRLRTSSIALHVLAAVLIIGAAISE
                     IWLSLDNVGQWLFGIYGAAAAIALLGMFAFYLAFVAELPPPPPKPLKPKKEKRRRGRR
                     KAAEDVEEEAADEAPESEEDATAEDETEDGTESASDEEPAEVTAEAEDPTAEEPEPTV
                     QDEEEPAANGSANGGLRNRRRRRLRGRVAVED"
     gene            8674..9027
                     /locus_tag="VFC01_01150"
     CDS             8674..9027
                     /locus_tag="VFC01_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78282.1"
                     /translation="MPEFNLNEPFALNGGQEALIPSEKLRLRFTDVVEDSRCPTQVDC
                     FWTGQARIAIAVQPDGGSPTNVEFNTNPAPGQTVKVVTVGKYGVELQSLDPYPQTPDP
                     IPFEDYRATLLVRKG"
     gene            complement(9024..10235)
                     /locus_tag="VFC01_01155"
     CDS             complement(9024..10235)
                     /locus_tag="VFC01_01155"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN78283.1"
                     /translation="MDSSAPRLDFTGTTPAVGYHHFHDDVSLNFQCNRWVQWIGPSAI
                     AEVTELARRCNTYPEWIDGFLGLAVDARDAERLLASAYYDRAAEFFMRPDDPRRDGAR
                     ARFAQTMRTLYDVSPDEVPYDGSALPAFDLRPPTETGSTIVVFGGFDSYVEEFLPMLA
                     TMFDAGHRVIAFEGPGQGAALEDRGLPMIPEWERPVAAVLDHYRLTDVTVVGESLGGG
                     LAIRAAAFEPRISRVVAMDILDDEFEVLARQIGRGVTPALRVLLALRARRIVNAVARQ
                     SIARKPVAEWGLQQGMHITGTSTAYDFMRSTTRVTTRRVSKLVTADVLLLAGADDHYV
                     PLKQLRRQAANLVNARSVTTRTFTAAEQASNHCQVGNIGAAVRVIQGWLELTAISHLA
                     EKGGVAQEFAR"
     gene            10322..10903
                     /locus_tag="VFC01_01160"
     CDS             10322..10903
                     /locus_tag="VFC01_01160"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HZN78284.1"
                     /translation="MSTHRRGEHVRRTVLAAAFDELAENGAGAATVAGVAQRSGVHET
                     TIYRRWITKEKLFVEAMLSRSADEIPTPDTGSIRDDLLAIVRAMTAYVTTPVGLAALH
                     VASLVVDEYQEGRQAFWAGRLDALRPVVDRGIARGELRPDTDAGLLLETLIAPLHSRL
                     LITGEPVDEQLGERIVDLVLDGARVQESGVRAL"
     gene            10908..11927
                     /locus_tag="VFC01_01165"
     CDS             10908..11927
                     /locus_tag="VFC01_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN78285.1"
                     /translation="MGISSLRRPLQALVIALAAVLALVSPATSVAAPADIAAAAAAVE
                     PAVVQITTKIDYQQAIGTGTGTVVDPGGVVLTNYHVVAGANTITGTVGGRDFPADLVG
                     YDRKHDIAVLQLRGAGGLPTAPIGDSGQVVVGEPTVGLGNARGVGAPLTHETGPVVAL
                     NRTVNAEDALTGSSEEVNGLIEVAADVRPGDSGGPLVNGAGQVIGVVTAATVNFQMGP
                     AGKGFAIPINDAMAVAGQIRSGAASGDVHIGQPTLLGVGVGTQQRSGGGIIVRDVMLG
                     GPAAQAGLATGDVLTNLDGTDLDSATTLTYVLDRHYPGDVVDLTWIDRSGQQRTGKAT
                     LVSGP"
     gene            11946..13013
                     /locus_tag="VFC01_01170"
     CDS             11946..13013
                     /locus_tag="VFC01_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN78286.1"
                     /translation="MHNTAPHRVVQWTTGNVGKSSVEAIAKNPNYELIGCFAWSKDKV
                     GRDVGELVGIEPLGVQATNDVDALLALKPDCVVYNPMWIDVDELVRILSAGVNVVSSA
                     SFITGHNLEAGRDRIEEACKQGGSTMFGSGVSPGFAELLAIVAANGCARVDKVTIAEA
                     SDTTFYDSPDTERPCGFGMPIDDPNLQPMMSQGTAIFAEAVRLVADALGVELDEIVCE
                     AEYAQTTADVEMASWTIKAGCVAGVFGSWQGRVGGKTVVDLNVRWRKGQTLDPDWKIE
                     GDPWTITIDGLPTVTMKVGFLPPPDFVENAKTMEDFMGLGHIITALPPIHAIPAVVAA
                     APGIVTYNDLPLTLPRGIVPN"
     gene            complement(13010..14299)
                     /locus_tag="VFC01_01175"
     CDS             complement(13010..14299)
                     /locus_tag="VFC01_01175"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN78287.1"
                     /translation="MTKPSLLRAEEGLPRGVQGAADSRFSNVVRVFAGLFPGKRFGGG
                     ALSVYIDGRPVVDVWTGWSDRAAEQPWTVDTGAMVFSATKGVAATVIHRLVDRGLLSY
                     DEPVATYWPEFGANGKDTITIRDVLRHRSGLSHLKGVSKDDLLDHKLMEERLAEASVD
                     HLRGWPAYHALTYGWLLSGLARAVTGQGMRELIREEVARPLDTDGLHLGRPPAGSPTK
                     VAQILAPQSTRTNPVFNFVAPKVAGLPLSGAFGAMFFPGIKSFVQGDIPFLDGEVPAA
                     NGVVTARALAKMYAAIANGGSIDDTEFLSDELAQGLIGNPRPWPDLNIVVPMPFHLGY
                     HESPIPGLLKGFGHIGLGGTLGWADPQSGSAFAFVHNRLLTPMVLDMASFAGLARPMR
                     SAITAARHAGALAVPRYGARYRDVGQQVSKKRVAGRR"
     gene            complement(14350..14787)
                     /locus_tag="VFC01_01180"
     CDS             complement(14350..14787)
                     /locus_tag="VFC01_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78288.1"
                     /translation="MQTIRKLLAALAIVAMLVGGISIAAALIFGDHSKPQQPTATPRT
                     TLAPPPSVPTPKEFTINVVVTEQNCEPGPGCAYKYTIEPKYIGFHPLPKTPFTVKYEV
                     QGGNQPQPGEFTVEGNQAKILKDVILEGPAAARLQAVVLQVVG"
     gene            complement(14885..15784)
                     /locus_tag="VFC01_01185"
     CDS             complement(14885..15784)
                     /locus_tag="VFC01_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78289.1"
                     /translation="MAEPVALAAQPQDLKTAQQAVPVEQNPPPAPEAQVTRIRDLIIA
                     PPNPDANAAPNVPAQNGVVAAERRVVLWQQEWVQYDEFYRPLIFNPFPDPLQVVYLLA
                     GAPRILLIPPLGRIVTEVPQLGSYNFTAVRLSAFGIPIDIAVGNFFGGGFVPGPGLPP
                     PPPPPPVITKANVPVKVKYTNAEYKPIRVKKVVDVGPDPTVGEHKVLLDGVAPAWGEW
                     KQGPTGEREFEVHKTQSFPGMEAPAEGPLPGDYPMQLVSDSSPTGLSAKDVALIGGAA
                     IVALLAVGAIVFTVYLGRRRPQH"
     gene            15786..16289
                     /locus_tag="VFC01_01190"
     CDS             15786..16289
                     /locus_tag="VFC01_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78290.1"
                     /translation="MSTPAAGSALVGPEVPPAGFGSAEVVPPGGLVPPGGLVPPVEVA
                     PVVSVVAVVDVVLDGTSPAGTLGDVDVVEEDVAEEVVVVVTPEFAVVVTPGFAVVVVT
                     AVVPPGASGIGAAVVVVVTISAGVGIGAAVVVVTISAGVGIGAAVVVTIAGSSVMIGA
                     CCRVVTI"
     gene            complement(16602..17444)
                     /locus_tag="VFC01_01195"
     CDS             complement(16602..17444)
                     /locus_tag="VFC01_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78291.1"
                     /translation="MGLFRQLRPGVLMITGMAVVAATVGVTAPDAEAFYIQNHERVTR
                     DALTPAGVDDATLNQIFVGPPPGAGAVGSDAFFSDEFRHIDDAKNPAEICDRAVQAWN
                     VFTPIVMSGSQPVGPNGSDLADGPAARAAFGGLAHALQDFYSHSNWVEDNIAAGQLER
                     LAPPLMPTCDPATLPADLHTGFFSMDFSSDFPLGGCPPAGPPPGFAECHSTLNKDGWD
                     TPRGILAVPGANPPMNYFDLAARLATRATTDLYWQVRGLVVNEHGECAAANLFQVDRH
                     QPCW"
     gene            17767..19836
                     /locus_tag="VFC01_01200"
     CDS             17767..19836
                     /locus_tag="VFC01_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN78292.1"
                     /translation="MKGTPFGRYELIELLGRGGMGEVWKAFDTATQRVVAVKVLPAQL
                     AADPMFEQRFRREAFTAAGLNNPHVVPIHNFGEIEGRLYVDMRLIEGEDLEHALAHGP
                     MDPDRAVMIVEQIASALNAAHRVGLVHRDVKPSNILLAEEDFAYLIDFGIARGAGETK
                     LTATGNVVGTWPYMAPERFTTGQSDTRSDIYALACVLYECLTSVRPFPGDSVEQQIAG
                     HLTTPPPRPSITRPGVSPQLDTVIATGMAKDPDERYGTTTELAHAAREALTAVIPKPP
                     PDPQHTAVGAAPSSPTEVHTVERVGQAWAPTEARMPTADDEAWTPTQAGPPDHVRKAW
                     AATEAGPPDHVEKAWAPTQNRPVEELHDTSPPEIHTPDQWPEAVRPLPWWQRMAVVIP
                     IAAVLVIAAVVTIFIVVIGGGSENRPPQAAALNGTYTVEFGADKRPNGQPYGNPSGGP
                     ETWVIKSACQTEGCVATASKVSGSQSAASTMVLDQIGGRWTAVSATHGTCMNSQAEFW
                     ETMSLRVQPDGTLAGEFIVRSTASCATDQQVKFTRVGDVKPDVSVADPQTQPPRVPSS
                     AQALHGKYQETDTYADGGRNAEVNFDIATYCLRTGDRCLSYWQNPNDTKTLVFSQNQW
                     VLANTAKDATCKNGSRAHGEVTLQYPLPQPAQDPVALLTGRGHYTVTGECPFNSDFDS
                     RVQRTGD"
     gene            complement(19833..20609)
                     /locus_tag="VFC01_01205"
     CDS             complement(19833..20609)
                     /locus_tag="VFC01_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN78293.1"
                     /translation="MAIDVARPRRIASLLGAATLTAGAVWIAAIGTPSVAADPCPDIE
                     VVFARGTGDALPGGPVGQAFVDSLRSKVAPRSVGMYGVNYAANMNFLLVTDGVNDANN
                     HVRFMIGNCPATRLVLGGFSQGAAVISVLVGATPNLAAIPGVGAIPGLGAIPGVGAIP
                     GVGAIPGVAAIPGLGGGGAPLPPEAADHVAAVAVFGNPVDKLLGPLNGLSPAYGFKTI
                     DLCNANDPICGQGDMDNRASHHQYVPGMTDQAAAFVAGLV"
     gene            20737..21411
                     /locus_tag="VFC01_01210"
     CDS             20737..21411
                     /locus_tag="VFC01_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN78294.1"
                     /translation="MSARRVVCFLGCGALMWAPFTMSSAHAAPCPDIEVVFARATTEP
                     PGVGQVGQAFVDSLRSQVGNRSVAVYAVDYPASEDFTNSASAGADNARAHVQAMAANC
                     PATRVVLGGYSQGALAIDLTTAIPLSMAGFTPAPIPPEVADHVAAVAVFGNPLDRYIG
                     MPLTAVSPSYADRTIDVCAAGDPICLPAGGMSTPTREEMFSDAHLSYVKSGLASQGAG
                     FAAGRL"
     gene            21536..21823
                     /locus_tag="VFC01_01215"
     CDS             21536..21823
                     /locus_tag="VFC01_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78295.1"
                     /translation="MAELVILATGVLGADASQAAYFRGFLADELAHVVFELAHSRARL
                     RECMERDQVVGLRGMARARRAVRDLEAKERELDRMIAAVDRRFSALWVREG"
     gene            complement(21820..22893)
                     /locus_tag="VFC01_01220"
     CDS             complement(21820..22893)
                     /locus_tag="VFC01_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HZN78296.1"
                     /translation="MNSPTTDARRRHDPDVRGFASDNYAGVHPEVLAALALANGGHQV
                     AYGDDAYTENLQGIIRDHFGASAEVFPVFNGTGANVLALQAITDRWGAVVCAESAHIN
                     VDEGGAPERMAGLKLLTVATPDGKLTAELIDRQAWGWADEHRAMPQVVSITQSTELGT
                     LYTPDEIRVICEQAHARDMRVHVDGSRIANAAASLDMPMGAFTSDVGVDILSMGGTKN
                     GALLGEAVVVLNRDAVSHMKHLRKLSMQLHSKMRFVSVQLEALLAKDLWLRSARHANE
                     MAQRLAEGVRAVDGVRVLYPVQANAVFARLPHDVSVRLQERFRFYFWDEAPGDVRWMC
                     SFDTTEADVDAFVAALKEEMARA"
     gene            complement(22890..23942)
                     /gene="ligD"
                     /locus_tag="VFC01_01225"
     CDS             complement(22890..23942)
                     /gene="ligD"
                     /locus_tag="VFC01_01225"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZN78297.1"
                     /translation="MPTPAEELDVDGVKVRLTNPDKVYFPKLGSGGTKRKLVEYYLAV
                     AGGPMLMALRNRPTHLQRFPDGIEGEEIYQKRIPQHAPDYLETCKVTFPSGRTADALK
                     VTHPSAIVWAAQMGTITLHPWQVRCPDTEHPDELRVDLDPQPGTGFREASAIAVDVLK
                     PVLEELGMVGYPKTSGGRGVHVFLRIKTDWDFIAVRRAGIALAREVERRAPDAVTTSW
                     WKEERGTRIFIDYNQNARDRTFASAYSARKTPIATVSMPLTWDELRDADPDDYTITTA
                     PDFVAGRDDPWEGIDSTAQSLDSLMDMVKADEERGLGDLPYPPNYPKMPGEPPRVQPS
                     KKVAANWDDEGNRKAT"
     gene            complement(23942..24997)
                     /locus_tag="VFC01_01230"
     CDS             complement(23942..24997)
                     /locus_tag="VFC01_01230"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZN78298.1"
                     /translation="MDIVDLPVLPPLEPMLAKAQAKVPPEAGVWSYEPKWDGFRALVF
                     RDGDKVIIQSRSGKDLGRYFPELEQALLDELAPRCVLDGEVVVPRDIGGRMRLDWESL
                     SQRIHPAASRIKMLAEQTPAHFIGFDALATGDRSLLKEPFRVRREALSEAVNEKQWCH
                     VTRTTEDPDKGAQWLDTFEGAGLDGVIAKRLDGPYLPGKREMVKVKHARDADCVAIGY
                     RIHKSGEGVGSILLGLHRDDGELQMVGGAASFTAKDRLKLLADLEPLREGDEAVDGAP
                     SRWNSAADKRWIPVRPEKVCEVQYDQMEGNTVHGQRFRHAVKFVRWRPDRDPQSCTFE
                     QLDVPITFDLYDVLEST"
     gene            complement(25897..26010)
                     /locus_tag="VFC01_01235"
                     /pseudo
     CDS             complement(25897..26010)
                     /locus_tag="VFC01_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
     gene            26065..>26796
                     /locus_tag="VFC01_01240"
     CDS             26065..>26796
                     /locus_tag="VFC01_01240"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZN78299.1"
                     /translation="MQLPVMPPVSPMLAKPVRSIPPGASYEPKWDGFRTICFRDRDEV
                     ELGSRNERPMTRYFPEVVEAIMAELPERCVVDGEIVIATDHGLDFEALQLRLHPAASR
                     VHMLAEQTPASFIAFDLLALDDDDYTDRPFSERRAALVDALAGSGSSVHLTPATADLA
                     TAERWFDEFEGAGLDGLIAKPLTITYQPDKRVMFKVKHERTADCVVAGYRVHKSGDDA
                     IGSLLLGLYKDDGQLSSVGVIGAFPM"
BASE COUNT         4606 a         8749 c         8783 g         4658 t
ORIGIN      
        1 gcgtacgacg gcagcatggc cggacccgag gacgccgaac tcggccttga tctgttgacg
       61 ccgcgcggca ccacctatct agccaccgcg cccggtgatc ttggcctggc ccgcgcctac
      121 gtgtctggcg acgtcgaacc gcacggtgtg caccccggcg atccctacga gctgctgaag
      181 gcgctcgccg agaagatgga cttcaagcgt ccgcccgcgc gggtgctggc caacatcatc
      241 cgctccatcg gcttcgaaca tctcaagccg atcgcgccgc cgccgcaaga agcactgccc
      301 cgctggcgtc gcatggcaga aggattgcgg cacagcagaa tccgcgatgc ggaggcgatt
      361 caccaccact acgacgtgtc aaacacgttc tacgagtggg tgcttggccc gtcgatgacc
      421 tacacctgcg cctgctatcc gcatccagac gcaacgctgg aggaggcgca ggagaacaag
      481 taccggctgg tgttcgagaa gctgcgcctg cggcccggcg accggctgct cgacgtcggc
      541 tgcggctggg gcggcatggt ccgctacgcc gcacgccacg gggtcaaggc gatcggcgtc
      601 acgctctcga aagaacaagc cgagtgggcg cagaacgcga tcgacgagca gggcctgtcc
      661 gacctgggcg aggtgcgcca cagcgactat cgcgatgtgc gcgaatccgg gttcgacgcg
      721 gtgtcgtcaa tcggtttgac cgagcacatc ggcgtgcaca actacccggc atacttccgg
      781 ttcctcaaat cgaaaatgcg gtccggtgcg ctgctgctca accactgcat cacccgccac
      841 gacaaccgaa ccggggccag cgcaggcggt ttcatcgacc gttacgtgtt ccccgacggc
      901 gagctcacgg gctcaggccg catcatcagc gaggcacagg acgtcggcct tgaggtggtg
      961 cacgaggaga acctccgtca ccactacgcg ctgacgctgc gtgactggtg ccgcaacctc
     1021 gtcgagcact gggatgaggc cgtcgaggag gtcgggctgc ccacagcgaa ggtgtggggc
     1081 ctgtacatgg ccggctcacg cgtgggcttc gagacgaatg tcgttcagct gcaccaggtt
     1141 ctggcggtca agctcgattc ccatggcggc gacggcaacc tgccgctgcg gccgtggtgg
     1201 acgccctaga gcaaaccaac accacaatcg agtagtcgac taccccggga tcgggtagtc
     1261 gactacgcgt gcccccagcc actcggccgg atcaccatgg tgtcgttccg cgcgctcaag
     1321 gcgaaacagt catcacggca ttctgccaag agggggtttt gaaatgccat caactaactc
     1381 gtacgacggc acttgcaccg accagtcagg gaacacctat ccggtcacca gcaaggaatc
     1441 ctacaatcag tgcatgctag tgggcggtac ttaccgcccg tacagcccgg atgccggcca
     1501 ccgcgtgtcc gccttggtgt tcgacgatca cgcccagatc gacaacgatg ccaacccgtt
     1561 cagcgcgcgc gcagtgggcg cgaggctgat gatctttccg atctgcgagg tgaatcgacg
     1621 gacccgtcgg tcccggctgc tgaacaagct cgatctgctt gaccgcaagt tcaccgacga
     1681 cgtgggtcga gtgctcgagg ccgaaccaga cctcaaggcc aaggtgtccc gactggtcgt
     1741 ggatattgcc tgggtcgccg cgcgggcact ctcagaagag ggcaaggcgg acgcgagcgg
     1801 gacggtcgtt tccgaggatc tgttcaaccg cgcgcgagaa ctcggcgccg aaattcaggc
     1861 caggacacaa aaccaggagc tgatcacagc gatcgacgat gccctcggtt ccagtgaaga
     1921 agtcatcggc gtcgccgtgt ctgaactgct cgccaagctc gagcaccgag cgaccgtgcg
     1981 cgccaggtcc tgagccacgg acgtgcatca tgggcctgat cgagatcgaa ctcagtcgaa
     2041 ccgaattcct gtcacttgca acgtttctcg tcaaacagtc gccgatcccc gacccaacct
     2101 tcgaggtgct gggaaacgat cggctggtgc gcagcttcga ctgcacaaac gtcacgctgg
     2161 ggaacgcccc acccgatctt gcaccgccgc caggcgtcgt cgccgcaaag gtggatgtga
     2221 agatcggcca tgcgtcggtt gccgagttgg aggccgacgc gaacgccaag accgtaaccc
     2281 aggccgtcgc atgggtgtac atatcgttgg tacccatccc ggggaagctg accttcgtgc
     2341 ccgtcggcaa gggccagcat ctactgatcg ccgatctcat tcgaattgac gttcaagggc
     2401 ggccggtcac cgggcgtttc gatgatcccg cgtcgccccg gtggggacgt caaccgttgg
     2461 gaacattgca gattccgctg cctgacatct tctctgacat tccgatcacc ggctcggcgg
     2521 tggtgctgtc ggcggctgcc gcaactctgc gagtcgccac agcaggacag aacgtcttcc
     2581 tgccggcggc ggaacggttg gtccaattcg gcgacaagtg gctgcttcgc gtgtcacccg
     2641 aagtgttcac cgaactgctg ctcacagcat tgatggattc ggtgacaccc gcgcctcccg
     2701 gcggcgcgac gttgattcaa ccgccctctg tgacgtgggg tccgaccgcg atcgggtggc
     2761 cgggcgggcc tccgtgggca gtagacctca acggctgggg ggccagcgga agttaccggc
     2821 tgacaaagca caaggcgtgt ggcgatgagg acgtcagcct tacggttcac accacgctgc
     2881 tgccggtgcc cgacgccgca agtagcaagc tgaccttcta tctggagatc agcgcgcaag
     2941 cggacgacgg caccaccttc aagtgctggc tgaacaacct cggcgtcgtg acgctggtcg
     3001 ccttcttcgt caaccccatc atcggaatcg gtgtcggcct tggcagttac gcgatcgtcg
     3061 gcggcaaggt tgacgacggt gtgggtgagg gtgtcacggg ccagagcggt ggaaaggggt
     3121 tcaagaccat cgggagcgga gacaacttca cgattcattc tctggaagtc gatctcaatc
     3181 tcggcgccct cgccaaggct ggagcgacga tgcagcacgg ggcctcggca gctgacgggg
     3241 ttaccttcgg cggagagttg aatctattgc cgctcagctt gatcgatcac tcttgcagct
     3301 tctggccgcc gaacggagcc accctgaaag ggacgtggtc gagcggctac aactgtaacc
     3361 agggctggca tgcctccttc gagctgcccc gcatccacat caccgataac gttgaagtgc
     3421 tgaacaccga gaagctgttc ggcaaagagg tcgccgtata cgacaccagt tgggttgtgc
     3481 cgtcggcgca ttggacgatc gattggccgg ggcccgctgt gattgaccag tacgtatcgg
     3541 tcatcggcga tctgtgggcc tacaccgggc cgggactgca cgtcggaagc cacttccttc
     3601 cgctgcctgc gggacatgtt ttcctccata cgagcgcagg tatcgcccgg ttcgagatcg
     3661 accacgttcc ggccccgccc gcacagccga ccccggccga actcattgcg atgaaagcca
     3721 actgcatgat gctcgtcgag cgctttgagg atgtccggtg gctggtcgac cctcccgact
     3781 tcgactacgg tcacgatccc ctgcggcact ggacggtcta cgtcgagaag gcgccgggtc
     3841 gaatgcagct ccaaattcac ggcgttcagc agggtgcacc gactcggcgg ctcgcatcct
     3901 tctcggcgga gtcgaatgag acgatcgcga tcgaggtcat caccgacggc cgcaccgagc
     3961 tgcgcatcga gcatgacctc gacggctcat tggagcacac ctcgatcaaa caacagtggc
     4021 tcctgccgac cagtacggtc gacttgaacg gcaaggcatc gacactcgaa ctagtcggtg
     4081 accgtctgga ggcgcgtccg gcgccgtccg tgatgtccat ttcgactgcg gccgttgcgg
     4141 aatcccggta cgccgacgtg ccgcctgcga gcgttgcgct gcccgacagc cgggtcgccg
     4201 cggtagatgg ccaccgactg gtggtgggtt acccgttcgg cgggacggcc gtgcatccgc
     4261 cggccccgtt ccgaagtagc gtcgagaccg caccttcggt caaaggtggc tgttgcggcg
     4321 aataacccta gggctcagcc gccgtcgatg cgacgggcgc cgagctcgtt ctgaaggagc
     4381 tccagcgccg cttcctccgg gtcgcggcgc tggacctccg aactgtcgtt gccagcctcg
     4441 gccagcatgt cctcttcgtc ctcgtcgcgc tgtggcggcg cggatacgac gggatcgtcg
     4501 ggctcgggtg cgggaggcgc ggcggcggca gttccgactt cgcaccggat cttccagtcc
     4561 acgccgagcg cgtccttgag cgcctcgcgg atgacgtcgg cattgcgttg ctccacaagc
     4621 cgtttggcca acggcgccga ctcgtggctg agcaccagag tgtcgccctc gacggcgcgg
     4681 acgatcgcgc ctgccagcat cacgtcgatg gtacggctgc gccggcggac cttctcacgc
     4741 acggtggtcc acatgctgcg caccgcggcc gcattgggct cgccccccat cgaaggcaca
     4801 gtcgcggccg gagcctccac tgctgggtcg gacggcgctc ggaccggagg cggtgacggc
     4861 ggcggctcgg gcgcaggcgg tgccggttcg gcctcgacct tgggctgcgg cgcgggttcc
     4921 ggcttcggca cggctttggg agtcggctca gtcctgctct tgcgggcgta ctgctttggc
     4981 ggcgcatctg gcgccgacgc ggcaccctcg ccggccggaa tggacatgtc gagccgcgtc
     5041 tcgatgcgct cgacacgctg cagcagcgcc gattcggtgt cgctggcaga gggcagcagc
     5101 agccgcgcgc acaccacctc gagcagcagc cgcggcgcgg tggcgccgcg catctcgccc
     5161 agcccggcgt gcaccacctc ggcataccgg gtcagcgtcg cggtgccgat ccgcgtggcc
     5221 tggtcgcgca ttcgctccag cacatcctcg ggcccgtcga ccacgccgcg ggcgatcgcg
     5281 tcgggcaccg actgcagcac gatcagatcg cggaaacgct ccagcaggtc ggtggcgaac
     5341 cgtcgcgggt cgtgcccggc gtcgatcacc gcctcgaccg cactgaagag cgccgcggcg
     5401 tccccagcgg ccaacgcgtc cacggcgacg tcgatcagcg ccacgtccgt cgcgccgagc
     5461 aatgacaacg cccgctggta gtccacccgg tttccttcgg caccggccag cagctgatcc
     5521 agcacggaca gggtgtcgcg cggcgaccca cccccggcgc gaatgaccaa cgggtacacg
     5581 gcgtcgtcga ccgtcacgtc ctccgaggcg cagatgcgtt ccagcagcga ccgcatggtc
     5641 cgcggggcca gcagccggaa cgggtagtga tgggtgcgcg accgaatggt gggcagcacc
     5701 ttctccggtt cggtggtcgc gaacacgaag atcagatgtt cgggtggctc ctcgacgatc
     5761 ttcagaagcg cgttgaatgc ctgcggagtg accatgtggg cttcgtcgat gatgaagatg
     5821 cggtatcgcg actcacccgg aacgtaaaac gcgcggtcac gcagctctcg cgcatcatcg
     5881 acgccgttgt ggctagctgc gtcaagctcc gtgacgtcca tgttgcccgg tccgttcggc
     5941 gccagtgcca cgcacgagtc gcacacgccg cacggggtgg gcgtcggacc ctgctcgcag
     6001 ttcagcgagc gggcgaggat gcgcgccgac gacgtcttgc cacagccccg tggcccggag
     6061 aacaggtagg cgtggttgat gcggccggcc gccagcgcgg tggagagcgg ttcggtgacg
     6121 tgttcctggc caaccacgtc ggcgaaggtt gccggtcggt acttccggta gagcgccacg
     6181 ctcagcaggc taccgacagc gtccgacgcc cgaacacgtc agcccttgag cagcgactcg
     6241 gtcgccgaca gcagcgcaca cgtggccagg ccgtcgatcg catcgcgcag ctcgggcagc
     6301 ccggggaatg acggcgcgat ccggatgttc ttgtcctccg ggtcttttcg gtacgggaag
     6361 gccgagcccg cctcggtgac ggcgatgccg gcgtccttgg cgagggccac ggtgcgcttg
     6421 gcggtgccgg gcaacacgtc gaggctgacg aagtagccgc ccttggggtc ggtccacgac
     6481 gcgaccttcg actcgcccag ccggtcggtg aggatctcct gcaccaaagc gaacttcggt
     6541 gccaacagct gctggtggcg ctgcatctgc aggcgcactc cgtctgcgtc gccgaagaac
     6601 cgcaggtgcc gcagctggtt caccttgtcc gggccgatgg atttcttacc cgcgtactgt
     6661 agataccacg cgatgttgcc cagcgagccg ccgaagaagc tgacaccggc gcccgcgaac
     6721 gtgatcttgg acgtcgacga gaagaccagc ggccggttcg ggttgcccgc cgcctcggcc
     6781 agccccagca cgtcgacctg ttgcacgaag tcgtgggtca gggtgtgaac gggatacgcg
     6841 ttgtcccata tcaaccggaa atcggttgcc gccgttcgca tctgcaccag ccggcggacg
     6901 gtgtcccacg aaaatgtgac gccggtcggg ttcgagtaga ccggcacaca ccacatgccc
     6961 ttgatggccg ggtcggaagc gacgagctcc tcgatcaggt cgacgtccgg gccgtcctcg
     7021 cgcatcggca cggtgatcat ctcgacgccg agcgtctcgg tgatcgcgaa gtgccggtca
     7081 tagcccggcg acgggcagag gaacttgacg acgggctcgg cgatccatgg ccgggtggag
     7141 tccacgccgc cgtgcagcat cgagtaggtc accacgtcgt gcatcagctc gaggcttgcg
     7201 ttgttgcccg cgatcaggtt cggcaccggg atgccgagca gctcgctgaa gattgcgcga
     7261 agctcgggca ggccgagcag gccgccgtag ttgcgagtgt cggtgccatc cccatcacgg
     7321 aatgcgtcgc gtccatctcc cggaagactc agcagcgcat tggagagatc gagctgggcc
     7381 ggtgacggct tgccgcgggt caggtcgagg ttgaggccct tggcctgcag gtcggcgtag
     7441 ttgcgcttct gctgctcgtg ctgcgcttgc agttcgtcac ggccgagaga ttgaaacgac
     7501 accgcgcgcc ttccaccaac gtcagatccg ggaaagaggg gaccccgcgc acccgccaga
     7561 gcccattgac ccttgctgcc ttccggccct gggggagttc acaggataga cgccgcgcgg
     7621 ggtccgtagc gagtgtaata ccagtgcgct ggacaactcg acgggcgcat cagccaggtc
     7681 ggctaccatg gcccgcggag gattcgccta gtggcctatg gcgctcgcct ggaacgcggg
     7741 ttgggttaac agccctcagg ggttcaaatc ccctatcctc cgcactcggc ccggggtgcg
     7801 accggctgaa gaagcaaccc ggtcgcgccc cggccaaagc cttgaggagg ggtatgggcc
     7861 gcactgttgc ggtggtttgg cacgcgtcgt tttctatcgc cgccggtgtc ggatacttct
     7921 tcttcgtgtt gccgcgctgg cccgaactgc tcggcgacac ctcgcacacc ctcggcactg
     7981 tgctgcgcat cgtcgccggc gccttgatcg ggcttgccgc gttgccggtg gtattcacgc
     8041 tgctgcgcac ccgccgaccg gagttcggta caccccagat ggcgttgagg ctgcgcacgt
     8101 cgtcgatcgc gctgcacgtg cttgccgcgg tgctgatcat cggggcggcg atcagcgaga
     8161 tctggctcag cctcgacaac gtggggcaat ggttgttcgg tatctacggg gccgccgccg
     8221 cgatcgccct gcttggcatg ttcgccttct atctggcgtt tgtggccgag ctgccgccgc
     8281 ctccgccgaa gccgctgaag cccaagaagg agaagcgtcg ccgcggtcgc cgcaaggccg
     8341 ccgaggacgt ggaagaagaa gccgccgacg aagcgccaga gtccgaagag gacgcgacgg
     8401 cggaggacga gaccgaagac ggcacggagt ccgcatccga cgaggagcct gctgaggtca
     8461 ctgctgaggc tgaggatccg acggccgagg agccggagcc gacggtccag gacgaggagg
     8521 agccggccgc caacgggtcg gccaacggcg ggctgcgcaa tcgccggcgc aggcggttgc
     8581 gcggccgtgt cgccgtcgag gattagcgtc tttgcggcgc tagcagctgt ggcgctgacg
     8641 agctgccacg tggacggatc cgcgcgagcc gacgtgcccg agttcaacct gaatgagccc
     8701 ttcgcgctga acggcggcca agaagcgctg attccaagcg agaaactgcg gctccggttt
     8761 accgatgtcg tggaggactc gcgctgcccg acgcaggtgg attgcttctg gacagggcag
     8821 gcgcgcattg ccattgccgt gcagcccgac ggcggcagtc cgacgaacgt ggagttcaac
     8881 acgaaccccg cgccgggcca gacggtcaag gtcgtcacag tcgggaagta cggcgtcgag
     8941 ctgcagtcgc tcgatcccta tccgcagacg cccgatccca tcccgttcga ggattaccgc
     9001 gccacgctgc tggttcggaa gggttagcgg gcgaattcct gagccacgcc gcccttttcg
     9061 gccaggtgac tgattgccgt gagttccaac cagccctgga tcacgcgcac ggccgcgccg
     9121 atgttgccga cctgacaatg attactcgcc tgttcggccg cggtgaacgt gcgtgttgtc
     9181 accgaccgcg cattgaccag gttggccgcc tgccgccgca actgcttcag cggcacatag
     9241 tggtcgtcgg ccccggcaag caacaagaca tctgctgtga ccaactttga gacgcgccga
     9301 gtggtgaccc tggtcgtcga gcgcatgaag tcgtaggccg tcgacgttcc ggtgatgtgc
     9361 atgccctgct gcagtcccca ctcggcaacg ggtttgcgcg caatcgattg tcgcgcaacg
     9421 gcattgacga tgcgtcgggc gcgcagtgcc aggagtacgc gcagtgcggg tgtgactccg
     9481 cggccgatct gacgggctaa cacctcgaac tcgtcgtcga ggatgtccat ggccaccacg
     9541 cggctgatcc gcggttcgaa ggcggccgcc cggatcgcga ggcctccgcc cagcgattcg
     9601 ccgacgacgg tcacgtctgt cagccggtag tggtcgagca cggcagccac cggtcgctcc
     9661 cattcgggaa tcattggcag gccgcggtct tccaacgcag cgccctgacc cggcccctcg
     9721 aaggcgatga ctcgatggcc cgcgtcgaac atcgtggcga gcatcggcag gaactcctcg
     9781 acatagctgt cgaatccgcc gaaaacgacg atggtgcttc cggtttcggt tggcgggcgc
     9841 aagtcgaagg caggcaacgc cgaaccgtca tacggcacct cgtcggggct gacgtcgtac
     9901 agcgtccgca tcgtctgcgc gaaacgcgcc ctggcgccgt cgcggcgggg atcgtcgggt
     9961 cgcatgaaga actcggccgc ccggtcgtag tacgccgacg ccaacagccg ttccgcgtcg
    10021 cgggcgtcga ctgccaaccc caggaagccg tcgatccatt ccggataggt gttgcagcgg
    10081 cgggccagtt cggtgacttc cgcgatcgcc gacgggccga tccactgcac ccatcggttg
    10141 cattggaagt tcaggctcac gtcgtcgtgg aagtggtgat agccgacggc cggcgttgtc
    10201 ccggtgaagt ccaggcgggg cgctgacgaa tccatggcat tctcctatcg caagtcaact
    10261 tgcgatagat catgcgcctg tttggaccgt attgcaagtg aacttgcgat agggtcgagc
    10321 gatgagcacg catcgccgcg gtgaacacgt ccgccgcacc gtgctggccg cggcattcga
    10381 tgagctcgcc gagaacggcg caggtgccgc gaccgtcgca ggggtcgcac agcggtcggg
    10441 ggtccatgag acgacgatct accgacgctg gatcaccaag gagaagctct tcgtcgaggc
    10501 aatgctgagt cgcagtgcgg acgagatacc cacaccggac accggatcga tccgcgacga
    10561 cctgctggcg attgtgcgag caatgactgc ctacgtgacg actccggtcg gcctcgccgc
    10621 gctgcatgtg gcgtcactgg tggtcgacga ataccaggag ggtcgccagg cattttgggc
    10681 gggcagactc gatgcgctgc gccctgtcgt cgaccgcggc atcgcccgcg gtgagctgcg
    10741 gccggacacc gatgccggac tgcttctgga gaccctgatc gcaccgttgc acagccggct
    10801 gctgatcacc ggcgagcccg tcgacgagca gttgggcgaa cgcatcgtcg acctggtcct
    10861 ggacggcgcg agggttcagg aatccggagt tcgggcacta taggcagatg ggcatctctt
    10921 ccttgcgccg tccgctgcaa gcgctggtaa tcgcactggc cgcggttctg gccctggtct
    10981 cacccgcgac ttcggtggca gctccggccg acatcgcggc ggccgccgcc gcggttgagc
    11041 ctgccgtcgt ccaaatcacc acgaaaatcg actatcagca ggcgatcgga acaggaaccg
    11101 gcactgtcgt cgatcccggc ggcgtcgtgc tgacgaacta ccacgtcgtc gctggcgcca
    11161 acaccatcac cggcaccgtg ggcggacgcg acttccccgc cgatctggtg ggctacgacc
    11221 gcaagcacga catcgcagtg ctgcagctgc gtggtgcggg cgggctgccg acggcaccga
    11281 tcggcgactc cggtcaggtg gtggtgggcg agccgaccgt cggtctgggc aacgcccgcg
    11341 gagttggcgc tccgctcacc cacgaaaccg gcccggtggt cgctctcaac cgcacagtca
    11401 acgccgaaga cgcgttgacg ggcagctccg aggaagtcaa cggcctgatc gaggtcgccg
    11461 ctgacgtgcg gccgggagat tcgggcgggc cgttggtgaa cggtgccggg caggtcatcg
    11521 gagtggtcac cgcagcgacc gtgaacttcc aaatgggtcc cgccggaaag ggtttcgcga
    11581 taccgatcaa cgacgcgatg gccgtcgccg gtcagatccg gtccggtgcg gcgtcgggcg
    11641 atgtgcacat cggacagccc acgctgctcg gcgtcggtgt cggcacccag cagcgcagcg
    11701 gcggcgggat catcgtgcgc gacgtgatgc tcggcggacc ggccgcacag gcgggtctgg
    11761 cgaccggcga cgtactgacc aacctcgacg gaaccgatct cgactccgcc accacactga
    11821 cctacgtgct ggaccggcac taccccggcg acgtcgtcga cctgacctgg atcgaccgca
    11881 gcggccagca gcgcaccggc aaggccaccc tggtttcggg cccgtaagca ccctatgctg
    11941 agcgagtgca caacaccgct cctcaccgag tcgtccaatg gacgacggga aatgtcggaa
    12001 aaagctcggt cgaggcgatc gcgaagaacc ccaactacga attgatcggg tgctttgcct
    12061 ggtcgaagga caaggtcggc cgcgatgtcg gcgagctcgt cggaatcgaa cccctgggcg
    12121 ttcaggccac caacgacgtc gacgcactgc tcgcgctgaa acccgactgc gtcgtctaca
    12181 accctatgtg gatcgacgtc gacgagctgg tccgcatcct ctctgcgggc gtgaacgtcg
    12241 tgtcgtccgc gtcgttcatc accgggcaca atctggaggc cggccgcgac cggatcgagg
    12301 aggcgtgcaa gcagggcggc tcgacgatgt tcggttcagg cgtcagcccc ggttttgccg
    12361 aactgctggc catcgtcgcg gccaacggct gcgcccgggt ggacaaggtc accatcgccg
    12421 aggcctccga caccaccttc tacgactcac ccgacaccga gagaccgtgt ggcttcggca
    12481 tgccgatcga cgatcccaac ctgcagccga tgatgtcgca gggcacggcg attttcgccg
    12541 aggcggtgcg tctggtggcc gacgcgttgg gcgtcgaact cgacgaaatc gtctgcgagg
    12601 ccgaatacgc gcagaccacc gcagacgtcg agatggcttc atggaccatc aaggccggtt
    12661 gtgtggcagg ggttttcggc agctggcagg gccgtgtcgg cggcaagacc gtcgtcgacc
    12721 tcaacgtacg gtggcgcaag gggcagacgc tcgaccccga ctggaagatc gaaggcgacc
    12781 catggacgat caccatcgac gggctgccga cagtcacgat gaaggtgggc tttctgccgc
    12841 cgcccgactt cgtcgagaac gccaagacga tggaagattt catgggactc ggccacatca
    12901 tcaccgcgtt gccgccgatc cacgcgatcc ccgccgtcgt cgccgccgcg cccggcatcg
    12961 tcacgtacaa cgacctgccg ctgacgctgc cgcgcggcat cgtgccgaat tagcgccgcc
    13021 cggctacccg cttcttcgag acctgctgac cgacatcgcg ataacgcgcg ccatatctcg
    13081 gtacggcgag cgcgcccgca tgccgtgctg ccgtgatggc actgcgcatc ggccgggcca
    13141 gccccgcgaa cgacgccatg tcgagcacca tcggggtcaa cagccggttg tggacgaaag
    13201 cgaacgcgct gcccgattgc ggatcggccc agccgagggt gccgccaagg ccgatgtgtc
    13261 caaatccctt gagcagcccg gggatcggcg attcgtggta gccgaggtgg aatggcatcg
    13321 gcacgacgat gttcaggtcc ggccagggcc tcggatttcc gatcaagccc tgcgccagct
    13381 cgtcggagag gaattcggtg tcgtcgatgc tgccgccgtt ggcgatggct gcgtacatct
    13441 tggccagagc gcgggcggtg acgacaccgt ttgcggcggg cacttcaccg tccaggaacg
    13501 gaatatcgcc ctgcacaaac gatttgatgc cggggaagaa catcgccccg aatgcaccgg
    13561 agagtggcag gccggccacc ttcggcgcga cgaagttgaa caccgggttg gtgcgcgtgc
    13621 tctgcggcgc gagaatctgt gcgaccttgg tcggtgagcc cgcaggcggc cggcccaggt
    13681 gcagaccgtc ggtgtcaagc ggtcgggcca cctcttcgcg gatcagctca cgcatgccct
    13741 ggcctgtcac cgcgcgcgcg agcccggaca gcagccatcc gtacgtcagc gcgtggtagg
    13801 cgggccaacc gcgcaaatgg tcgaccgacg cctcggccaa ccgctcctcc atcagcttgt
    13861 ggtccagcag gtcgtccttg ctgacaccct tcaggtgcga tagcccggac cggtgccgca
    13921 gcacgtcgcg aatggtgatg gtgtccttgc cattggcgcc gaactcgggc caatacgtcg
    13981 cgaccggctc gtcgtaggac agcaggccgc gatcgaccag acggtgaatg acggtcgccg
    14041 cgacgccttt cgtcgcggag aacaccatcg cgcccgtgtc gacggtccag ggctgctcgg
    14101 ccgcgcggtc ggaccacccc gtccacacat cgacgaccgg tcgcccatcg atgtacaccg
    14161 aaagcgcgcc accaccgaac cgcttgccag gaaacagacc cgcgaacacg cgcacgacat
    14221 tggagaaccg cgaatcggcc gcaccctgga caccgcgggg caaaccttcc tcagcgcgga
    14281 gcagcgacgg ctttgtcata cagctaacaa tgcgtcgagc acgctgccgt gcatgcggaa
    14341 tcgcaatatt cagccgacga cctgcaggac gaccgcctgc aaccgggcgg ccgccggtcc
    14401 ttcaaggatc acgtccttga ggatcttcgc ctgattgccc tcgacggtga actcaccggg
    14461 ctgcggctgg ttgccaccct gcacctcgta cttgacggtg aacggggtct tcggcaatgg
    14521 atggaagcca atgtatttcg gctcgatcgt gtacttgtag gcacaaccag gacccggctc
    14581 gcagttctgc tcggtgacga cgacgttgat cgtgaactcc ttgggtgtgg gcaccgaggg
    14641 cggcggcgcg agcgtcgttc gcggcgttgc ggtcggctgc tgcggtttcg agtggtcgcc
    14701 gaagatcagg gcagccgcga tgctgatgcc gccgacaagc atcgcgacga tcgcgagcgc
    14761 cgcgagcaac ttgcggatgg tctgcatcta ctccccttgt cggccgcatc tggcattcga
    14821 aatcgctccg catgccagat gttaggcgct gcgcggcgtc gggaagcaaa ggtcggcagg
    14881 cgagtcagtg ttgcggacgc cgacgtccaa ggtagacggt gaagacgatc gcacccacgg
    14941 ccaacaacgc cacgatggcc gcgcccccga tcagggcaac gtccttggcg ctgaggccgg
    15001 tcggagacga atcgctgacc aattgcatcg ggtagtcgcc gggcagcggt ccctctgccg
    15061 gcgcctccat gcccgggaac gactgcgtct tgtgcacctc gaactctcgc tcgccggtgg
    15121 ggccctgctt ccattcgccc caggcaggcg ccacgccgtc gaggagaacc ttgtgctcac
    15181 cgacggtggg atccgggccg acgtcgacga ccttcttcac gcggatcggc ttgtactccg
    15241 cgttggtgta tttcaccttg accggcacgt tggccttcgt gatcaccggc ggtggcggag
    15301 gcggaggcgg cagaccggga ccggggacga agccgccgcc gaagaaattg cccactgcga
    15361 tgtcgatggg tatgccaaaa gcactgagcc gcaccgccgt gaagttgtac gaaccaagtt
    15421 ggggcacttc ggtcacgatg cgtcccagcg gtggaatcaa cagaatccgt ggcgcaccag
    15481 ccaggaggta gaccacctgg agcggatcgg ggaacgggtt aaatatcagc ggccggtaga
    15541 actcgtcgta ctgcacccac tcctgctgcc agagcaccac cctgcgctcg gcggcaacaa
    15601 cgccgttttg cgcgggaacg ttgggcgcgg cattcgcgtc cggattcggc ggagcgatga
    15661 tcaagtccct gatccgggtg acctgagcct ccggcgccgg gggcgggttc tgctcgacgg
    15721 gcaccgcctg ctgggcagtc ttgaggtcct gcggctgtgc ggccagcgcg actggctcag
    15781 ccaccttgtc cacccccgcg gccggctcgg cgcttgtcgg acctgaggtg ccaccagcgg
    15841 gattcggctc cgcggaagtc gtgccgcccg ggggactcgt gccgcccggg ggactcgtgc
    15901 cgcccgtcga agtcgcgccc gtcgtctcgg tggtcgcggt ggtggacgtc gtgctcgacg
    15961 gcaccagtcc cgcaggcacg ctcggagacg tggacgtcgt ggaggaggac gtcgcggagg
    16021 aagtggtcgt cgtcgtcacg cctgagttcg ccgtcgtcgt cacgcctggg ttcgccgtcg
    16081 tcgtcgtgac ggctgtcgtg cctcccggtg ccagtggcat cggcgcggcc gtcgtcgtcg
    16141 tcgtgacaat ctcggcgggc gtcgggatcg gcgcggccgt cgtcgtcgtg acaatctcgg
    16201 cgggcgtcgg gatcggtgcg gccgtcgtcg tcacgatcgc aggatcctcg gtgatgatcg
    16261 gcgcctgctg ccgcgtggtg acgatctgag gttgctcggt cacgaccggc gcctgcgtca
    16321 cgaccggcgc ctgcgttacg accggcgctt gtggaaccac cggcgcctga atgaccggcg
    16381 gcgcctcgta ggtcggctcg tactgcggaa ccgtaggcac ggcttcggtt gtcgttacgt
    16441 agatgtcggt aggttcggcg aggactgggg aaactcccgc gctaagcgcg gtcaacgcga
    16501 tcgcagcacc tgacgccagg acaactgcta cgccacgagc acggcaaata ccaagtggca
    16561 caagcatcgt ggctcaactc ctctccacgt tgcggtcaag gtcaccagca gggttgatgc
    16621 cgatcgacct ggaacagatt ggcggcggcg cattcgccgt gctcgttgac gacgaggccg
    16681 cggacctgcc agtacaagtc ggtggtggct cgggtggcca gtcgcgctgc gaggtcgaag
    16741 tagttcatcg gggggttggc gccgggcacc gcgagaatgc cccggggggt gtcccagccg
    16801 tccttgttca aggtcgagtg gcattccgcg aaaccgggcg gcggacctgc cggcgggcac
    16861 cccccgagcg gaaagtccga cgagaaatcc atcgagaaga agccggtgtg taggtcggct
    16921 ggaagggtgg ccggatcaca tgtgggcatc agcggtggcg ccagacgctc tagctgtccg
    16981 gcggcgatgt tgtcctcgac ccagttggag tgcgagtaga agtcctggag tgcgtgagcc
    17041 aggccgccaa acgccgcacg agctgccggc ccgtccgcga ggtcgctgcc gttgggcccg
    17101 acgggctgcg atccgctcat tacgatcggc gtgaagacgt tccaggcttg gactgcgcgg
    17161 tcgcagatct cggcgggatt cttcgcgtcg tcgatatggc ggaactcatc actgaaaaac
    17221 gcgtcgcttc cgacggcgcc ggcaccaggc ggcgggccga cgaatatttg gttcagggtg
    17281 gcgtcgtcga cgccggcagg tgttagcgca tcacgcgtga cgcgctcgtg gttctggatg
    17341 tagaaggctt cggcatccgg tgcggtaacg ccgacggttg cagcaacgac ggccatcccc
    17401 gtgatcatca gtacgcccgg ccggagttga cggaaaagcc ccatcacaaa tccccctgat
    17461 gatccatttc agctaagtgc cccagatgat cgggattgtg tcagcgcgca gccaggaatt
    17521 tcacagaatc ggcaaaaata ccggctcaac ttcgcatctt gttgcgcgga gagggcaact
    17581 gcacggaccg tttcgacttc tgaccagcaa atttttacgc tttcgtccgc tgcactcgtg
    17641 gaccgatagt tgtcgcactg gttgcgtgct caagcggtag cggccccgca atacccattg
    17701 cgtcgcgaat cggcggccta tggtgatggc tgtgaacctg ctggtttgct gccgtcggga
    17761 ggcttcgtga agggaacgcc cttcggccgc tacgaactga tcgaactctt gggtcgtggt
    17821 ggcatgggcg aggtctggaa ggcgtttgac acagccaccc agcgggtcgt tgctgtcaag
    17881 gtcctgccgg cgcaactggc cgccgatccg atgttcgagc agcggttccg gcgcgaggcc
    17941 tttacagctg cggggctcaa caatcctcac gtggtgccga tccacaactt cggcgagatc
    18001 gagggtcgac tctacgtcga catgcgcctg atcgaggggg aagacctcga gcatgccttg
    18061 gcgcacggcc cgatggaccc ggaccgggcc gtgatgatcg tcgagcagat tgcctcggcg
    18121 ctcaacgctg cgcatcgggt cggcttggtg catcgcgacg tcaagccctc caacatcctt
    18181 cttgcggagg aagatttcgc gtatctcatc gacttcggga tcgcccgtgg tgcaggcgaa
    18241 accaaattga ccgcaaccgg caacgtcgtg ggcacctggc cctacatggc gcccgagcgt
    18301 ttcaccaccg gccaaagcga cactcgctca gacatttacg cgctcgcctg cgtgctctac
    18361 gagtgcctga catcggttcg gccgtttccg ggcgacagcg tcgagcagca gatcgccggt
    18421 cacctgacga ccccgccgcc gcggccctcg atcacccgcc cgggcgtgtc accgcaactc
    18481 gacacggtga tcgcaacggg catggcgaaa gatcccgacg aacgctatgg caccacaacg
    18541 gaattggctc acgcggctcg agaggcactg accgctgtca taccgaagcc gccgcctgat
    18601 ccacagcaca cggcggtggg ggcagcgccc tcgtctccca ccgaggtcca cacagtcgag
    18661 cgtgttggcc aggcctgggc acccaccgag gctcgcatgc ccacagctga tgacgaagcc
    18721 tggacaccca cccaggcggg tccgcctgac cacgtcagga aagcgtgggc ggccaccgag
    18781 gccggtccgc ccgaccatgt tgagaaggcc tgggccccca cacaaaaccg gccggtcgag
    18841 gagctccacg acacctcgcc gcccgaaatc catacccctg atcagtggcc cgaagccgtg
    18901 cgcccgttgc catggtggca acgaatggcg gtggtgatcc ccatcgctgc ggtcctggtg
    18961 atcgctgctg tcgtcacaat cttcatcgtc gtgatcggag gcggcagcga aaacagaccg
    19021 ccccaggcgg cagctctcaa cggcacatac acggtcgagt tcggcgcgga taagaggcct
    19081 aacggacagc catacggaaa cccatcgggc ggacccgaga cgtgggtgat caagtcggcg
    19141 tgccagaccg agggatgcgt ggcgaccgca tcgaaggtca gcggatctca gtctgcggcg
    19201 tcgacgatgg tgctcgacca gatcggcgga cgctggacag ccgtcagcgc gacgcacggg
    19261 acgtgcatga acagtcaggc cgaattctgg gaaacaatgt cgctgcgggt tcagccggac
    19321 ggaacgctgg ccggtgaatt cattgttcgg tcgacggcaa gctgtgccac cgatcagcag
    19381 gtgaagttca cgcgcgtcgg ggacgtgaaa cccgatgtct cagttgccga tccccagacc
    19441 cagccgccgc gggtgccatc atcggcccaa gcgcttcacg gcaagtacca agagacggac
    19501 acctatgccg acggcggtcg caacgcggaa gtgaatttcg acatcgcgac ctactgcctg
    19561 cgcaccggtg atcggtgtct gagctattgg caaaatccca acgacacaaa gactttggtc
    19621 ttctcccaga atcagtgggt cttggcgaac acggcgaaag acgcgacgtg caagaacggc
    19681 agtcgtgccc acggcgaggt aacccttcag tacccgctcc cccagccggc gcaggatccg
    19741 gtcgcactgc tcaccgggcg gggccactac acggtcaccg gtgagtgtcc gttcaacagc
    19801 gacttcgact ctcgcgtgca gcgcaccggc gactagacca gccctgcgac gaacgccgcg
    19861 gcctgatcgg tcatgccggg aacgtattgg tggtgcgacg ccctgttgtc catgtcgccc
    19921 tggccgcata tcgggtcgtt ggcgttgcac aggtcgatcg ttttgaaccc gtacgcagga
    19981 ctcaggccgt tcaacggacc gagcagtttg tccaccgggt tgccgaacac cgctacagcc
    20041 gccacgtgat cggcggcctc gggaggcagc ggcgcccctc cgccgccgag gccaggtatg
    20101 gcagcgacgc cgggtatcgc acccactccc ggtatcgcac ccactcccgg tatcgcaccc
    20161 agtcccggta tcgcacccac tcccggtatg gctgccaggt ttggcgtggc accgacaagg
    20221 acgctgataa cagcggcacc ctgtgagaag ccgccgagca ccagccgggt cgcgggacag
    20281 ttcccgatca tgaaccgcac gtgattgttg gcatcgttca cgccgtcggt caccagaaga
    20341 aaattcatgt tcgccgcgta gttgaccccg tacattccga ccgacctcgg cgccaccttg
    20401 gagcgcagcg agtcaacgaa agcctggccc accggtcccc cggggagcgc atcaccggtg
    20461 ccgcgcgcga agacgacctc gatatcggga cacggatcgg cggcgacgga aggggtgcct
    20521 atggccgcaa tccacacagc gcccgccgtc agtgtggcgg cgccaagcaa gctcgcgatc
    20581 cgacgtggcc gcgccacgtc aatggccacc actcgatagt aggcagcggt tgtcagagcc
    20641 gtgtgcactt gcgaaatccg atcagcgctt tgcgcccagt tccttgcgct ggcaacaacg
    20701 ttcccttccg ccgcgacgat gtgccagcat ggattcgtga gcgcacgccg agtcgtgtgt
    20761 ttcctaggtt gcggggcgtt gatgtgggcg cccttcacaa tgtcgtctgc acacgccgcg
    20821 ccctgccccg acatcgaggt ggtgttcgcc cgcgcgacga ccgagcctcc tggtgtcggc
    20881 caagtcgggc aggcgtttgt cgactcactt cgctcgcaag tcggaaacag atccgtcgcc
    20941 gtgtatgcgg tcgactatcc cgcctccgaa gacttcacga attccgcgtc ggctggcgcc
    21001 gacaacgccc gagctcatgt ccaggcaatg gccgcaaact gtccagcaac cagagtcgtg
    21061 ctcggtggat attcccaagg cgcgctcgcg atagatttga ctaccgcgat cccactatca
    21121 atggctgggt ttacacccgc cccgataccg cccgaggtcg cggatcacgt tgcagccgtt
    21181 gccgttttcg gcaaccccct ggacagatac atagggatgc cgctgacagc ggtcagcccg
    21241 tcgtacgcgg acaggacgat cgacgtgtgt gccgctggcg atccgatctg cttgcccgcc
    21301 ggcggtatgt cgacgccgac gcgagaagag atgttctccg atgcgcacct gtcgtacgtg
    21361 aagtccggat tggcgagtca aggcgcgggt ttcgccgccg gtcgacttta gctttcgctg
    21421 caacggcgac ccgcacactc cacagacagt tcaggccgtc gctcgggccg tggtagtacc
    21481 tatcgattct tggagttgag tggcactggg ctaagagttc ggttaagtta cggcggtggc
    21541 cgaacttgta atcctggcga ccggggtgct tggagcggat gcctcgcagg ccgcctactt
    21601 tcggggtttc ctggccgatg agctcgcaca cgtcgtattc gaattggccc acagccgtgc
    21661 tcggttacgc gaatgtatgg aacgcgatca ggttgttggg cttcgcggga tggcgcgggc
    21721 gcggagggcg gttcgtgact tggaagccaa ggagcgcgag ttggatcgaa tgatcgctgc
    21781 ggtcgaccgc cggttctccg ccctgtgggt tcgggagggt taggcgcgcg ccatctcttc
    21841 cttgagcgct gcgacgaagg cgtcgacgtc ggcctcggtg gtgtcgaacg agcacatcca
    21901 gcggacgtct ccgggggcct cgtcccagaa gtagaaccga aaccgctcct gcagccgcac
    21961 gctgacgtcg tgcggcagcc gggcgaagac cgcgttggcc tgcaccgggt aaaggacccg
    22021 cactccgtcg accgcgcgca ccccctcggc gagccgctgg gccatttcgt tcgcgtgtcg
    22081 ggcgctacga agccacaagt ccttggccag cagcgcctcc agctgcaccg agacgaagcg
    22141 catcttggaa tgcagttgca tcgacagctt gcgcagatgc ttcatgtggc tgaccgcgtc
    22201 ccgattgagc accacgaccg cctcgcccaa cagcgcgccg ttcttggtgc cccccatcga
    22261 caggatgtcc acgccgacgt cggacgtgaa cgcccccatc ggcatgtcca gtgacgcagc
    22321 cgcattggcg atccgcgagc cgtccacgtg cactctcatg tcgcgggcgt gggcctgttc
    22381 acatatcacg cggatctcgt ccggcgtgta cagcgtgccc agctcggtgc tctgcgtaat
    22441 ggagacgacc tgcggcatgg ctcggtgttc gtcggcccaa ccccaggcct gccggtcgat
    22501 cagctcggcc gtgagcttgc cgtcgggcgt ggcaactgtt agcagcttga gcccggccat
    22561 ccgctcgggc gcaccgccct cgtcgacgtt gatgtgtgca ctctcggcac ataccactgc
    22621 gccccaacga tcggtgatcg cttgcagtgc aagcacattc gcacccgtgc cgttgaagac
    22681 gggaaacacc tctgcggaag cgccgaagtg gtcgcggatg atgccctgca ggttttcggt
    22741 gtaggcgtcg tcaccgtagg cgacctggtg tccgccgttg gccaacgcca gggccgcaag
    22801 gacctccggg tgaacacccg cgtagttgtc actcgcgaag ccgcgaacgt cggggtcgtg
    22861 gcgacgccgg gcatctgttg taggagagtt cacgtcgcct tccggttacc ctcgtcatcc
    22921 cagttcgccg ccaccttctt gctcggttgc acgcgaggcg gctcgcccgg catcttcgga
    22981 tagttgggcg gatacggcag gtcgcccagc ccgcgttcct cgtcggcttt gaccatgtcc
    23041 atcaatgaat ctaatgactg tgctgtcgaa tcgattccct cccagggatc gtcgcggcct
    23101 gcaacgaagt cgggtgccgt ggtgatggtg tagtcgtcgg ggtccgcgtc gcgcagctcg
    23161 tcccacgtca acggcatcga tacggtcgcg atcggcgtct tgcgcgccga gtacgccgac
    23221 gcgaacgtgc ggtcgcgggc gttttggttg tagtcgatga agattctcgt gccgcgctct
    23281 tccttccacc acgacgtggt caccgcatcg ggggcccgcc gctcgacctc gcgggccagc
    23341 gcgataccgg cccggcgcac cgcgatgaaa tcccagtccg tcttgatccg caagaagaca
    23401 tgcacgccac ggccgccgga ggttttcgga tatccgacca tgccgagttc ctcgaggacc
    23461 ggcttgagca catcgaccgc gatcgcgctg gcctcccgaa aacccgtgcc cggctgtgga
    23521 tccaggtcga cgcgcagctc gtcgggatgt tcggtgtccg ggcagcgcac ctgccacgga
    23581 tgcagcgtga tggtgcccat ctgcgccgcc catacgatcg ccgacggatg agtcaccttc
    23641 agcgcgtcgg cggtgcggcc ggacggaaac gtcactttgc aggtttccag ataatccggg
    23701 gcatgctgcg ggattcgctt ctggtagatc tcctcgccct cgatgccgtc ggggaaccgc
    23761 tgcagatgcg tcggccgatt cctcaatgcc atgagcatgg ggccgcctgc cacagcgagg
    23821 taatactcga cgagcttgcg cttcgtcccg ccggagccga gcttcgggaa gtacaccttg
    23881 tccgggttgg tcagccggac cttgacgccg tcgacatcga gttcttctgc gggcgttggc
    23941 attacgtaga ctccagcacg tcgtagaggt cgaaggtgat cggtacgtcg agctgctcga
    24001 aggtgcagct ctgcggatcg cggtccggcc gccatcgcac gaacttcacc gcatggcgaa
    24061 atcgctggcc gtgcacggtg tttccctcca tctggtcgta ctgcacttcg cacacctttt
    24121 cgggacgcac cgggatccac cgcttgtcgg cggcggagtt ccaccggctc ggcgccccgt
    24181 cgaccgcttc gtcgccctcg cgcaacggct cgaggtcggc cagcagcttg agtcggtctt
    24241 tcgcggtgaa cgacgcggca ccgccgacca tctgtagttc gccgtcgtcg cggtgcagcc
    24301 cgagcagaat cgatccgacg ccctcgccgc tcttgtggat gcggtagccg atggccacgc
    24361 agtcggcgtc gcgggcgtgc ttgaccttga ccatctcccg cttgcccggc agatacgggc
    24421 cgtccaaccg cttggcgatc acgccgtcca gccctgcgcc ctcgaaggtg tcgagccact
    24481 gcgcgccctt gtcggggtcc tcggtggtcc gggtgacgtg acaccactgc ttctcgttca
    24541 ccgcctcgga cagcgcttcc cggcggaccc ggaacggctc cttcaacaac gacctgtcgc
    24601 cggtagccag cgcgtcgaag ccgatgaaat gcgcgggcgt ctgctcggcc agcatcttga
    24661 ttcgactcgc ggcagggtga atgcgctggg acaacgactc ccagtccagc cgcatgcgcc
    24721 cgccgatatc acgcgggacg acgacctcgc cgtcgagcac gcagcgcggc gccagctcgt
    24781 caagcagcgc ctgttccagc tctgggaagt agcggccgag gtccttgccg ctgcgcgact
    24841 gaatgatgac cttgtcgccg tcgcggaaca ccagcgcgcg aaagccgtcc cacttcggct
    24901 cgtaggacca cacccccgct tcgggtggca ccttggcctg ggccttggcc agcatcggct
    24961 ctagcggtgg cagtacgggt aagtccacga tgtccatact cacgtagacc gccaaagccg
    25021 ggcaatgtca tcgccgcagt tgaggcggca cccgtctcct caacagcgcg aaggacgagg
    25081 gccatgagct tgtcagggtc cacttcagtg gtgggtcttg atcctcgcgt cgacctgatc
    25141 acggcgccac ggcgccgctg ttgggtccgc ggggcgccgg tatcagtgga ttcgaaggct
    25201 gtcccggcgg gttctgcagc accggcggag tcgtggtctg tcccggctct tggggcaagt
    25261 tttctgcctc cgccggcgga gcaacgcact cacctatgtt cgcgtcccaa tctccgccag
    25321 aattcgcaca gcagcccttt tcaaagcgga tgtattcatc gccttgcttg tgcggatcta
    25381 ggccgggcgg ccaagtagcg atgcacttgt cgtatgcatc gagatcccat acgcgttcgg
    25441 cgcccgcggt tgcggctgat ccgattgcga tgcctgccat gagcgcggtt gcggccagac
    25501 attgactaat gcgggcgcag attgtggtgt agttcgttga tgtcatttct ggtccttggt
    25561 gtcgaggtgg tgttggtgcc agccggtgat gtccggcctg aaattgtgtg tttgcgggag
    25621 gggttcccga ctgcgcggat cgtgcgtcgc ggcgccgtag ctgagtgcga gaagcgcttc
    25681 atgccgtgat gctcgtccac tgggagtggc ggcacatgag gcgtttgcct caacttctgc
    25741 cgtgaccgtg tcgaatggcg ccaacgacga gtcactcgtc cacgttgagg gcgccaagac
    25801 tagccaacgt cgaatggttg atggcggtga cactgggtcg ctagcgagac cctctggctt
    25861 cgaacctggg caatcttgac aagttcagtg aagacagccg tcccacttcg gctcgtagga
    25921 ccacaccccc gcttcgggtg gcaccttggc ctgggctttc gccagcattg gctctaacgg
    25981 tggcaacacg gggaggtcca caatctccat actcgactag accgtcgagc ccgggcaatc
    26041 tcatcggcgc gcgagaatga accggtgcag ctgcccgtga tgccgccggt gtcgccgatg
    26101 ttggccaaac cggttcggtc gatcccacca ggcgcctcgt atgagccgaa gtgggacggg
    26161 ttcaggacaa tctgcttccg cgaccgcgac gaggtcgagc tgggcagccg caacgaacgg
    26221 ccgatgaccc gatacttccc ggaagtggtg gaagcgatca tggccgagct gccggagcgc
    26281 tgcgtggtcg acggagagat cgtcatcgcc accgaccacg gtctggactt cgaggcgctc
    26341 cagctgcggc tgcacccggc cgcgtcgcgg gtgcacatgc tcgccgagca gacgccggcc
    26401 tcgttcatcg cattcgactt gctggccctc gatgatgacg actacaccga tcggccgttc
    26461 agcgagcgcc gcgccgccct cgtcgacgcg ttggccggct ccggctcctc ggttcacctc
    26521 accccggcga ccgcagacct ggccaccgcc gagcgctggt tcgacgagtt cgagggcgcc
    26581 ggcctcgatg ggctgatcgc caagccgctg acgatcacgt accagccgga caagcgcgtc
    26641 atgttcaagg tcaagcatga gcgaaccgcc gactgtgtgg ttgccggcta ccgcgtgcac
    26701 aagtcgggtg atgacgcaat cggctcgctg ctgctcggcc tctacaagga cgacggccag
    26761 ctttcctcgg tcggcgtcat cggcgccttc ccgatg
//
LOCUS       DASRJA010000025         7949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2780787,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000025 DASRJA010000000
VERSION     DASRJA010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2780787"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1649)
                     /locus_tag="VFC01_01245"
     CDS             complement(174..1649)
                     /locus_tag="VFC01_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009476318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZN78300.1"
                     /translation="MQTYNHYIDGGEVEPAGREWLDSIDPYLGEPWAKIARGTAADVD
                     RAVTAAARAMRDGPWASTTATERGKLMVRLADLVAENAERLAEIEVRDNGKLLAEMRG
                     QLNYHPEWWRYFGGLADKVQGAVMPIDKPDVMAFTRHEPIGVVAALTAWNSPLLFVAW
                     KCSAAIAAGCSVVVKPSEFASVSTLEFAKLTREAGFPDGVFNVVTGLGAEAGAALVDH
                     PDVAKVTFTGSDATGAKIYAQAARSLKRVSLELGGKSPNIVFDDCDLDAAAAGVISGI
                     FAATGQTCIAGSRLLVQNSIREEFTDRVVKLGESARKGNPMLPDTNIGPVTTLPQYAK
                     ILDYIDVAKAEGARCVLGGGPATELPGGQFVEPTIFVDVKPDMRIAREEVFGPVLSVI
                     GFDDEADAVRIANDTIYGLAAGVWTGDIGRAVRMSSALKAGTVWVNTYRAISFMMPFG
                     GMKHSGFGRESGIEAIKEYLETKSVWLSYATGAPANPFIMR"
     gene            complement(1665..3419)
                     /locus_tag="VFC01_01250"
     CDS             complement(1665..3419)
                     /locus_tag="VFC01_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN78301.1"
                     /translation="MTQTDGLLFDPNTYDPQQFDEETRRLLRATIDFFEGQGKKRILD
                     DDLAAQWPADFVEFVKREKLFATFLTPSEFADGNPDKRWDAARNAALSEILGFYGLTY
                     WYTEQVTILGLGPIWQSENKDAKLKAADDLEAGHVMAFGLSERDHGADVYNTDMVLTP
                     ASEKDRADGILYRATGEKYYIGNGNVASMVSVFGRRGDIVGKDAGDGYVFFAADSQHE
                     NYHLIDNVVHMQIYVSTFRLENYPVREEDILHTGVEAFAAALNTVNVGKFNLCTCTIG
                     MTEHAFYEAITHAQNRILYGKPVTEFSHVRANFVDAYARLVAMKLFSQRSVDYFRSAS
                     LEDRRYLLFNPMTKAKVTMEGETVLRALHDVIAAKGYEKNTMFREVAELIGTLPRLEG
                     TVHVNVGLVLKFMPNYMFNPAEYPEIPTRNDPTDDTFFWNQGPTRGAGKVQFADWTPV
                     YENHARIPNVARFYEQAKAFATLLATAAPDAEQQKDLDFMLNVGHLFSLIVYGQLILE
                     QATLTGLDDDIVDQIFDFQIRDFSTYAVALHGKPSSTQGQQDWAVSAIRKPVSDADRF
                     DRVWQRVKAYDGAYEMRP"
     gene            complement(3430..5181)
                     /locus_tag="VFC01_01255"
     CDS             complement(3430..5181)
                     /locus_tag="VFC01_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZN78302.1"
                     /translation="MSDGVGLSVGASNLAAVVVGRTAVTRSPVVTLYPHRPPEVGVPS
                     ENPNLTERGLIITDFVDRVGDPVGIVAADGSSHMADKVLADALRAMLYTVGRPPAGPV
                     AITYPAHWRPAAVDALRNALASVPALQEAPLISDASAALTALQDDPGVPTRGVIALCD
                     FGGTGTSITLVDATNGFEPIGPTVRHTDLSGDLIDQALLTHVIDDLSAAGTIDLSGTS
                     AIGSLSRLRAQCRGAKERLSTASVTSLVAELPGHRSDVRLTRNELDEAIRAPLADFVS
                     VVQETVERSGIPTAELVAVASVGGGARIPIITTTLSEHFRVPVTTTGQPELTAAIGGG
                     LKAVRGTVDEGMTSMAAAPGAVAAAAAATAMAPEVGPQDAPQSSTFGALAWSDADDVP
                     DVAPTDPYDYGGEADGGGFTDYARPQMQFDHEPQEYEAAPVPWYRKPAVFLGAGVVAL
                     LVALAAAVLFVMRDDSAPTPASTTTAVTTTPPPTTTAAAPPPASPAPPPPQTQAPQTR
                     TVTREAPPPPQTVTEAPPPPPETSEAPPPPPPTTEAPPPTTEAPPTTQAPWSPTAPYP
                     TIPGLPWVPAPQAPAQP"
     gene            complement(5244..6272)
                     /locus_tag="VFC01_01260"
     CDS             complement(5244..6272)
                     /locus_tag="VFC01_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN78303.1"
                     /translation="MVIAEIVKHPLFELVGVGVSNPDKVGRDVGEICALGQNLGLAAT
                     DDVDALIALKPDALVHYGPTAAHADDNIALITRFLRAGIDVCSTAMTPWVWPTMRLNP
                     PNWIEPITVACELGGSSCFTTGIDPGFANDLFPMTLMGLCSEVRKVRASELLDYTNYE
                     GDYEFEMGIGKPPEYRPLLENPDVLVFAWGATVPMIAHAAGIMLDEITTTWDKWVTPT
                     ERKSAKGIISPGNVAAVRFTINGMYQGETRIQLEHVNRIGNDAAPDWPSGTENDVYRV
                     DIEGTPSIFQETAFRFTDGSGRDAAAAGCLATGLRALNAVPAVNDLSPGWVTALDLPL
                     IPGVGTIR"
     gene            6484..7227
                     /locus_tag="VFC01_01265"
     CDS             6484..7227
                     /locus_tag="VFC01_01265"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HZN78304.1"
                     /translation="MTSSLTGRKALVTGGSRGIGAEIVRRLAADGAAVAFTYGSSTAE
                     AEKLAAEVAESGRTAVAIQADSGDPEQVARSVDETVAELGGLDILVNNAGVAYVDNAE
                     SFPLEQFDRLVAVNVRAVFVAIQRAIPHLGEGGRVINIGSINADRVPAPGLSVYAMTK
                     GAVASLTRGLARELGPRGITVNNIQPGPIATDMNPEEGEFADWARPMTSLGRYGQTSD
                     IASAVSYLAGPESGYITGANWNVDGGFTV"
     gene            complement(7230..>7949)
                     /locus_tag="VFC01_01270"
     CDS             complement(7230..>7949)
                     /locus_tag="VFC01_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HZN78305.1"
                     /translation="SDSIYLHAIVNRGFGNVIWTEIWKSSDSGISWTSMGEDARFPAD
                     LHGGYAQCWSWDYEPDDGWVYVVATGFQRDKGIIRMRVRPRHIGQPSRYVSWGHTAGR
                     WKWGEVATPITPKGEKWGELTFRRMAQGKWVLGGFLASRYALGYRVVDSPIANMHTTE
                     LQIPVVGSSWHDENHSKNRVAQLYGGYVLPGSRFDVQGGFGLVVSQWNTAVGWPYRAM
                     QFKAALKDTTKTVQPPDPINL"
BASE COUNT         1321 a         2563 c         2631 g         1434 t
ORIGIN      
        1 tgaacctcga gttcgacgtg gtcgtcgacg gcaaccggtt gaacggacac tcgcgtgccg
       61 ggcggttgcc acgctcggcg gtgacgggtg tgcgccgaag acgacgacga gtgaggatcg
      121 cagcggggga cgagcgagga ccggagtgag gaggagtcga gacatgagcg ctgttagcgc
      181 atgatgaacg gattggccgg tgcaccggtg gcgtatgaca gccacacgct cttggtctcc
      241 agatattcct tgattgcttc gatcccactc tcgcggccga acccggaatg cttcatcccg
      301 ccgaacggca tcatgaagct gatcgcgcgg taggtgttca cccaaaccgt tccagccttc
      361 agcgccgatg acattcgcac ggcccggccg atgtcaccgg tccaaacacc ggcagccagg
      421 ccgtaaatcg tgtcgttcgc gatgcgcacg gcgtcggctt cgtcgtcgaa tccgatcacg
      481 gacagcactg gaccgaacac ctcctcgcgc gcgatccgca tgtccggctt cacgtcgaca
      541 aagattgtcg gctcgacgaa ctgaccgccg ggaagctcgg tggcgggtcc gccgcccagc
      601 acgcaccgcg cgccttccgc cttggcgacg tcgatgtaat ccaggatctt ggcgtactgc
      661 ggcagtgtcg tcaccggacc gatgttggtg tcgggcaaca tcggattgcc tttgcgcgcc
      721 gactcgccca gctttacaac gcggtcggtg aactcctcgc gaatcgaatt ctgaaccagc
      781 agacgggaac ccgcgatgca cgtctgaccg gtggccgcaa agatgcccga aatcacgccc
      841 gctgccgcag cgtcgaggtc gcagtcgtcg aacacgatgt tcggtgactt gccgccgagt
      901 tcaagcgaga cgcgcttgag gcttcgcgcc gcctgcgcat agattttcgc tcccgtcgcg
      961 tcggacccgg tgaacgtgac cttggcgacg tcgggatgat cgacaagggc ggccccggcc
     1021 tctgcgccca gaccggtcac cacgttgaac accccgtctg ggaatccggc ctcgcgcgtt
     1081 agcttcgcga actcaagtgt tgacaccgat gcgaactcgg atggcttcac cacgacgctg
     1141 catccggcgg caatcgcggc cgagcacttc cacgcgacga acaacagcgg cgaattccac
     1201 gctgtcaggg cagcgacaac gccaatcggt tcatgcctgg tgaacgccat cacgtccggc
     1261 ttgtcgatcg gcatcaccgc gccctggact ttgtcggcca aaccgccgaa gtagcgccac
     1321 cattcggggt ggtaattgag ctggccgcgc atctccgcca ggagcttccc gttgtcgcga
     1381 acctcgatct ctgctagccg ctcggcgttc tctgcgacga gatcagccaa ccgcaccatc
     1441 agcttgcccc gctcggttgc ggtcgttgac gcccacggac cgtcacgcat ggcgcgcgcc
     1501 gcagcggtta ccgcgcgatc gacgtcggcc gccgtgccgc gcgcaatctt cgcccacggc
     1561 tcacccagat acgggtcgat gctatccagc cattcgcgac cggccggctc aacctcgcca
     1621 ccatcgatgt aatggttgta cgtctgcatg ccgaccctcc cgcgtcatgg gcgcatctcg
     1681 tacgccccgt catatgcctt gacacgttgc cagacccgat cgaagcgatc ggcatcggac
     1741 accggcttgc ggattgcgga aaccgcccag tcctgctggc cctgcgtcga gctcggcttg
     1801 ccgtgcagcg caacggcgta cgtgctgaag tcgcggatct ggaagtcgaa gatctgatcg
     1861 acgatgtcgt cgtccagacc agtcagtgtg gcctgctcca ggatgagttg cccgtagacg
     1921 atcaatgaga acaggtgccc gacgttgagc atgaagtcca gatccttctg ctgttcggca
     1981 tccggagccg cggtggcgag aagcgtcgca aacgccttgg cctgttcata gaatcgcgcg
     2041 acattcggga tgcgcgcgtg gttctcatac accggcgtcc agtcggcgaa ctgcaccttg
     2101 cccgcgccac gggtcggtcc ctgattccag aagaaggtgt cgtcggtggg atcgttgcga
     2161 gtcggaatct ccggatactc agcggggttg aacatgtagt tcggcatgaa cttcagcacc
     2221 aggccgacgt tgacgtggac ggtgccttcc aatcgcggca gcgtgccgat cagttctgcg
     2281 acttcgcgga acatggtgtt cttttcgtag cccttggcgg cgatcacatc gtgcagcgca
     2341 cgaaggacgg tttcgccctc catcgtgacc ttggctttcg tcatggggtt gaagagcagg
     2401 tagcgacggt cctcgaggct cgcgctgcgg aagtagtcga cggagcgctg gctgaacagc
     2461 ttcatcgcga ccagtcgggc gtacgcgtcg acgaaattcg cccgcacatg ggagaactcg
     2521 gtgacgggct tgccgtagag gattctgttc tgcgcgtgag tgatcgcctc gtagaaggcg
     2581 tgttcggtca tgccaatcgt gcaggtgcat aggttgaact tgccgacatt gaccgtattc
     2641 agcgctgccg caaacgcttc cacgccggtg tgcaagatgt cttcttcccg caccggatag
     2701 ttctccagcc ggaacgtgct cacgtagatc tgcatgtgca ccacgttgtc gatcaggtgg
     2761 tagttctcgt gctggctgtc ggcggcgaag aacacgtacc cgtccccggc gtccttgccc
     2821 acgatgtcgc cgcgccggcc gaagaccgac accatgctgg cgacgttgcc gttgccgatg
     2881 tagtacttct ctcccgtcgc gcggtacaga atgccgtcgg cgcggtcctt ctcgctggcc
     2941 ggggtgagca ccatgtcggt gttgtagacg tcggcgccgt ggtcacgctc ggacaacccg
     3001 aaggccatca cgtggccggc ctccaggtca tcggctgcct tgagcttggc gtccttgttc
     3061 tcgctctgcc agatcggccc gagtcccagg atggtgacct gctcggtgta ccagtaggtc
     3121 aggccgtaga acccgaggat ctccgacaaa gcagcattgc gggcggcatc ccagcgcttg
     3181 tctgggttgc catcggcgaa ttccgacggc gtcagaaacg tcgcgaacaa tttctcccgc
     3241 ttgacgaact caacgaagtc cgcgggccac tgcgcggcca ggtcatcgtc cagaatgcgc
     3301 ttcttgccct ggccttcgaa gaaatcgatc gtcgcgcgca gcagccgccg cgtctcctcg
     3361 tcgaactgct gcgggtcgta ggtgttcgga tcgaagagca agccgtcggt ctgcgtcatg
     3421 aacgcgagcc tatggctgcg caggtgcctg cggcgccgga acccacggca gacccgggat
     3481 cgtcgggtac ggcgcagtgg gactccacgg cgcctgcgtc gtcggaggag cctccgtggt
     3541 cggcggcggt gcctccgtgg tgggaggcgg tggcggcggc gcctcgctgg tctccggcgg
     3601 aggcggcggg gcctcggtga cggtctgcgg gggcggcggc gcctcccggg tgacggtgcg
     3661 ggtctggggc gcctgcgtct gcggtggagg cggcgccgga gaggccggag gtggtgccgc
     3721 tgcggtcgtc gtcggcggcg gcgtggtggt gacggcagtg gtggtcgacg cgggcgtcgg
     3781 ggcgctgtcg tcgcgcatca cgaacaacac cgccgcggca agggccacca gcagcgcaac
     3841 cacacctgcg cccaggaaaa cggcgggctt gcggtaccac ggcaccggcg ccgcttcgta
     3901 ctcttgtggt tcgtggtcga actgcatctg cgggcgcgca tagtcggtga aaccgccgcc
     3961 atcggcctcg ccgccgtagt cgtacgggtc ggtgggcgcg acatcgggga cgtcgtcggc
     4021 gtccgaccat gccagcgcgc cgaaggtgct cgattgcggc gcgtcctgcg gaccgacctc
     4081 gggtgccatg gcggtagccg cggcggctgc ggcgacggca cccggggccg cggccatcga
     4141 cgtcatacct tcgtcgaccg tgccgcgcac cgctttgagt ccgccaccga tcgcggccgt
     4201 caactcgggc tgtccggtgg tggtgacggg cacgcggaag tgctcggaca gtgtggtggt
     4261 gatgatcggg atgcgggcgc cgccgccgac ggaggctacg gcgacgagtt cggccgtcgg
     4321 aattccgctg cgctccaccg tctcctggac cacgctgacg aaatcggcaa gcggggcgcg
     4381 gatcgcttcg tcgagctcgt tgcgggtcag ccgaacatcg ctgcggtgcc cgggcagttc
     4441 ggccaccagt gaggtgactg atgcggtgga cagccgctcc ttggccccac ggcactgggc
     4501 ccgcagccgg ctcagtgagc cgatcgccga ggtgccggac aagtcgatgg tgcccgccgc
     4561 cgacaggtcg tcgatgacgt gggtcaacag cgcctggtcg atgaggtcgc cggacaggtc
     4621 ggtgtggcga acggtcgggc cgatcggctc gaacccattg gtggcgtcga caagcgtgat
     4681 gctggtgccg gtgccgccga agtcgcacag cgcgatgacg ccgcgagtcg gcacgccggg
     4741 gtcgtcctgc aatgcggtga gcgccgcgct tgcgtcggag atgagcggcg cctcctgaag
     4801 cgccggcacc gacgccagcg cgttgcgcag cgcgtcgacg gcggctggac gccagtgggc
     4861 ggggtaggtg atcgcgacgg gtccggccgg cggtcgaccg acggtgtaga gcatggcccg
     4921 cagggcgtct gcgagcacct tgtcggccat gtgcgaggag ccgtcggcgg cgacgatgcc
     4981 aacggggtcg ccgacgcggt ccacgaagtc ggtgatgatc aggccgcgct cggtgaggtt
     5041 cgggttttcg ctgggcacgc ccacctcggg cggccggtgc gggtagagcg tcactaccgg
     5101 cgaccgcgtc accgcggtcc ggcctactac gacggccgcc aggttggagg caccaaccga
     5161 gagccctacc ccgtcagaca tgtcactccg tctcgatgaa aaccctatcc ggtattcaat
     5221 cttgtctgac ggtgttcgcg ttactagcga atggtcccca caccgggaat cagtggcaaa
     5281 tccaaagccg tcacccagcc tggcgaaagg tcgttgacgg cgggcaccgc gttgagcgcg
     5341 cgcagcccgg tcgccaggca ccccgcggcg gccgcgtccc gtcccgagcc gtcggtgaag
     5401 cggaacgcgg tctcctggaa gatgctcggc gtgccttcga tgtcgacgcg atacacgtcg
     5461 ttctcggtgc ccgacggcca gtccggtgcg gcgtcattgc cgatccggtt gacatgttcg
     5521 agctgaatgc gggtctcgcc ctggtacatg ccgttgatcg tgaaccgcac ggccgcaacg
     5581 tttccgggcg aaatgatgcc cttggcagac ttgcgctcgg tgggcgtcac ccacttgtcc
     5641 cacgtcgtgg tgatctcgtc gagcatgatg cccgccgcat gtgcgatcat cggaaccgtt
     5701 gcgccccagg cgaataccag cacatcgggg ttttccagca gcgggcggta ctcgggaggc
     5761 ttgccgatgc ccatctcgaa ctcgtagtcg ccctcgtagt tggtgtagtc aagcagttca
     5821 gatgcgcgca ccttgcggac ctccgagcac agacccatca gcgtcatcgg gaacaggtcg
     5881 ttggcgaagc ccgggtcgat gccggtggtg aaacacgacg atccgcccag ctcgcaggcc
     5941 acggtgatgg gctcgatcca gttcggcggg ttgaggcgca tggtgggcca cacccacggc
     6001 gtcatggccg tcgaacacac gtcgatgccg gcccgcagga accgggtgat cagcgcgatg
     6061 ttgtcgtcgg cgtgcgccgc ggtgggaccg taatgcacca gcgcgtcggg tttcagcgcg
     6121 atgagggcgt cgacatcgtc ggtggcggcc agaccgaggt tctgtcccag agcgcagatt
     6181 tcgccgacgt cgcggcccac cttgtcgggg ttgctcacgc cgacaccgac gagttcgaac
     6241 agtggatgct tgacgatctc ggctatcacc atcttgccga cgaatccggt accccagacg
     6301 acgacgcgct tcttggcgga atccttcacg gccttcaccc taggcgggct cgatttgacc
     6361 gcgtcaacat ttgaggctct gccacatttg atgacgctct cgagaatcat gcagtgaacc
     6421 tgggccgcca gtgacgccga ccatagcggg aatgatccgg ccgcgacttg cctttacgaa
     6481 ggcatgactt catcactgac tgggcggaaa gcactcgtga ccggcggatc gcgcggtatc
     6541 ggagcggaga tcgtgcgacg gctggccgcc gacggcgccg cggtggcgtt cacatatggg
     6601 tcctcgacag cggaggccga aaagctggcg gccgaggtcg cagaatcggg tcgcaccgcg
     6661 gtggcgattc aggcggacag cggtgacccc gagcaggtcg ccaggtcggt cgacgaaacc
     6721 gtcgccgagt tgggcggcct ggacatcctg gtcaacaacg cgggcgtggc gtacgtcgat
     6781 aacgccgagt cgttcccgct cgagcagttc gaccggttgg ttgccgtcaa tgttcgggcc
     6841 gtattcgtcg ccatccagcg ggctattcct caccttggcg aaggtggccg cgtcatcaat
     6901 atcggcagca tcaacgccga ccgcgtgccc gcgcccggcc tgtcggtgta cgcgatgacc
     6961 aagggcgccg tcgcaagcct cacccgaggg ctggcccgtg agctcgggcc tcgcggcatc
     7021 accgtcaaca atattcagcc cggcccgatc gccaccgaca tgaaccccga agagggcgaa
     7081 ttcgctgatt gggcccggcc gatgacgtcc ctcggccgct acggtcagac cagcgacatc
     7141 gccagcgcgg tcagctatct ggcgggaccg gagtcgggct acatcaccgg cgccaactgg
     7201 aacgtcgacg gcggcttcac cgtctgacgt cacaaattga tcggatccgg tggctgcacc
     7261 gtcttcgtgg tgtccttgag cgcggctttg aattgcattg cccgataagg ccatccgacg
     7321 gcggtgttcc actgtgacac gaccagcccg aaaccgccct gaacgtcgaa ccgcgagccc
     7381 ggcagcacgt agccgccgta cagttgcgcg acccggttct tggaatggtt ctcgtcgtgc
     7441 cacgacgacc cgaccacggg gatctgaagt tcggtggtgt gcatgttggc gattggcgag
     7501 tccacgaccc ggtagccgag cgcatacctc gaggccagga atccgccgag cacccacttg
     7561 ccttgtgcca tacggcgaaa tgtcagctct ccccacttct cccctttggg cgtgatcggt
     7621 gtggcgacct ctccccactt ccagcgcccg gcggtgtggc cccagctcac atatcgtgac
     7681 ggctggccga tgtgccgggg ccggactcgc atccggatga tgcccttgtc ccgttgaaat
     7741 cccgtcgcca ccacgtacac ccagccgtcg tcgggctcgt agtcccacga ccagcactgc
     7801 gcgtagccgc catgcaggtc tgcgggaaac ctggcgtcct cgcccatgga cgtccaggag
     7861 atgccgctgt cgctggactt ccagatctcg gtccagatga cgttgccaaa acctcgattg
     7921 acaatggcgt gtaagtaaat tgaatcgct
//
LOCUS       DASRJA010000026         4698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3634755,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000026 DASRJA010000000
VERSION     DASRJA010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3634755"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..891
                     /locus_tag="VFC01_01275"
     CDS             85..891
                     /locus_tag="VFC01_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78306.1"
                     /translation="MTPALHRPAVAEKAAEGLRLALVSAVAAEVRWAFTPPRSWLSGV
                     AANLIVALVWLLVQPLHLPRHHDWVILIGTYFSSFILADVTTTNLLGTDHIRVRKALR
                     DGVPLWRVLLVKNLALVVIVGLPTLVAATAMTLWLETPARLAVTIPNVAVPIVSWLGV
                     GNLVSVLFPVGGERLIHRWGQRRDRRTTSRWIAHLVLPYALYYVADPTGGIEHNFFWR
                     QVPEAIAPILGPESRGFVHIGISAAVWAIATAVALLFFSRRGLQIVSTRK"
     gene            1007..1945
                     /locus_tag="VFC01_01280"
     CDS             1007..1945
                     /locus_tag="VFC01_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN78307.1"
                     /translation="MSINDGPVADADQTTDQRTLLSDPVGMWRVAATPLRALESARAV
                     VRMVGRAAAVHAYEVGALASTVAMVPLSVVGGVLEPASSVSPLTKGHAAPTARPVLLV
                     HGFGGTRSSWSVIANALSDRGIVVDTIAYSPMGNSVEQLADELVAAVQRTLSRSGADK
                     VNLVGHSLGGVVIAQAIAGGGLGGLVDTVITLGSPFGGSPWAALLPFGDLLRALRQGS
                     PQLRRLASGPVPVGVRWLSVTAALDIIVPGLRSVPAHADVETITVDGIGHLGMLISPR
                     VVDYIIAALTAHGPSVAAESHSRRLPMAARQAATAS"
     gene            2122..3216
                     /locus_tag="VFC01_01285"
     CDS             2122..3216
                     /locus_tag="VFC01_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN78308.1"
                     /translation="MTTPFRVGVIGANPDRGWAKDSHIPALRSLENVQLAAVATTSRA
                     SADAAAAVFGVRAAYDDPHAMVTAPDIDIVSVCVRVPYHRDLVLAALAADKHVYCEWP
                     LGRDGGEAAEMAAAAGMRAVHVAIGLQAHMNSAARRAADLIAAGAIGRVLTARIFSST
                     AGFAPHLPATHAYLNKLESGANLITILGGHTLDLAILVLGAIDSLDALTTLQHPTVTL
                     TDTGEQIRRTAPDHLLVQARMSSGCALAVEVAGDRPPDTPFTFEVVGTEGRILLAGGH
                     PNGFQAGRLTLSLDGERQWVDEPPDTLPAAAVNVAAMYRALSRDVTFGEHTAPDFDHA
                     LRLTHLMDDVVQSAGSGHRLTRKDWPAGGS"
     gene            complement(3584..>4698)
                     /locus_tag="VFC01_01290"
     CDS             complement(3584..>4698)
                     /locus_tag="VFC01_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN78309.1"
                     /translation="GGTVWSFRSLREVGVRTPARLDWPGTVLFGGGLTALLAAITYGI
                     QPYRGSPTGWTNPWVLGSITLAVVALVALYFVESRAPAPMLDFRLFRSKLFALGNLAG
                     LMASVGRGGLQFMLIIWLQGIWLPLHGYSFESTPLWSGIYMLPMTLGFLLAGPLSGAL
                     SDRFGSRPFVIGGLMLMAMTFVALALIPVDFDYRLFAVLIFLNGVGGGIFTSPNTAAI
                     MSSVPAAERGAASGIRATVTNAGMSLSIGIFFSLMIAGLAHSLPTTLLAGLQQQGVPA
                     TVAHDVANLPPAESLFAAFLGYNPIAELLGSSGVLQQRGVNAAVLTGKQFFPQLISGP
                     FHTGLVVVFTAAAVMMVIGAAASWVNPGHTVNDSGD"
BASE COUNT          805 a         1510 c         1571 g          812 t
ORIGIN      
        1 gtcggtcgta ctgacgccct gacagtcatt taaccaatga ggtctgggtg cgctggacga
       61 tgggccggcg cgatcctggg tcgggtgact ccggccctcc accgtcctgc cgtcgcggaa
      121 aaggccgcgg agggtctgcg actcgcactg gtttcggcgg tggctgccga ggttcggtgg
      181 gcattcacgc cgccgcgatc gtggctttcc ggtgtagccg ccaacctaat cgtggcactg
      241 gtgtggttgc tggtccagcc gttgcatctg ccccgtcacc acgactgggt catcctcatc
      301 ggcacctact tctcgtcgtt cattctggcc gacgtcacca ccaccaatct gcttggcacc
      361 gaccacattc gggtgcgcaa ggcgttgcgc gacggggttc cgctctggcg cgtgctgctg
      421 gtgaagaacc tggcgctggt cgtgatcgtc ggactgccga ctcttgttgc ggcgacggcc
      481 atgacgctct ggctcgaaac acccgcacgg cttgcggtga cgattcccaa cgtcgcggtg
      541 ccgattgtgt cctggctcgg ggtaggcaac ctcgtgtcgg tgctgtttcc tgtcggcggc
      601 gagcgactga tccatcgctg gggacaacgc cgagaccgtc gcaccacgag caggtggatt
      661 gcccacctgg tgctgccgta cgccttgtac tacgtcgccg accccaccgg cggcatcgag
      721 cacaacttct tctggcgtca ggttccggag gccatcgctc cgatattggg ccccgaaagt
      781 cgcggtttcg tccacatcgg catctcggcg gccgtctggg ctatagcgac cgctgtggcc
      841 ctgctgttct tctcgaggcg cggcctacag atcgtgagca cccggaaata ggctgagcgg
      901 cttcacacga atccgtcgtt gacgttgtca gcgggcacat cgccgtcggt cgtatcctcc
      961 gccacgctcg gatcgtgggg ccgctgcggg aaggagctca atgcttatga gcatcaacga
     1021 cggacctgtt gcggacgctg atcaaacgac cgatcagcgt acgctgctgt ccgaccccgt
     1081 gggaatgtgg agggtggcgg cgacgccgct gcgcgcactc gagagtgctc gcgccgtggt
     1141 ccggatggtt gggcgcgccg cggcggtgca tgcatacgag gtgggggcac tggcttcaac
     1201 agtggcaatg gtgccgctat ccgtcgtcgg tggcgtgctc gagccggcga gcagcgtgtc
     1261 cccgctgacg aaagggcacg ccgcacctac ggcccgtcca gtgctgctcg tccacgggtt
     1321 cgggggtacg aggtccagct ggtccgtgat cgcaaatgcg ctgagcgatc gaggcatcgt
     1381 ggtcgacacg atcgcctaca gcccaatggg aaactctgtg gagcagcttg ccgacgaact
     1441 cgtcgccgcg gtgcagagga ccctgtctcg aagcggcgcg gacaaagtga atctggtcgg
     1501 acacagtctg ggcggtgtcg tcatcgccca ggccatcgcg ggcggcggcc tgggcgggct
     1561 ggtcgacacc gtcatcaccc tgggctcccc gttcggggga tcgccctggg cggccctgct
     1621 gccgttcggg gatctgctcc gggcgttgcg acaaggttca cctcagttgc ggcggttagc
     1681 ctccggtccg gtacccgtcg gcgtgcggtg gttgtcggtt accgctgcac tggacatcat
     1741 cgtcccgggc ctgcggtcgg tgcccgccca cgccgacgta gagaccatca cggtcgatgg
     1801 catcggccat ctcggcatgc tcataagtcc gcgggttgtc gactacataa tcgctgcctt
     1861 gactgcgcac gggccgtccg tggcagcgga gtcgcatagt cgtcggttac caatggccgc
     1921 acgacaagcc gcgacggcct cttaactgag ggccatacat gtgggcggcc atctcgagcg
     1981 gctaccgtgg ttgttcggca gcacatcgac ggcgggccgc agcgaacgta acacttgaat
     2041 cgtggcagct gaggatggat cgccgcatgg cgtccggcgc aacttcatat gccccaccca
     2101 ccgactcggg cggaggatgg tatgacgacg ccatttcgcg tcggtgtcat cggcgccaac
     2161 ccagatcgcg gctgggccaa ggattcccac attccggcgc tgcgcagcct cgagaatgtg
     2221 cagctggcgg cggtggcaac caccagccgt gccagcgccg acgccgccgc tgcggtgttc
     2281 ggcgtgcgcg ctgcttatga cgatccccac gccatggtca cggcgcctga catcgacatc
     2341 gtctcggtgt gcgtaagggt gccgtatcac cgcgacctgg ttctggcagc gcttgcggcg
     2401 gacaagcacg tttattgcga gtggccgctg gggcgtgatg gcggcgaagc ggccgagatg
     2461 gcagccgccg cggggatgcg cgcggtccac gtggcgatcg gcctgcaggc gcacatgaac
     2521 tcggccgcgc gtcgcgcggc tgacctgatc gccgccggcg cgatcggccg ggtgttgacc
     2581 gcccggatct tctccagcac cgccggtttc gcgccgcacc taccggcgac acacgcctac
     2641 ctcaacaagc ttgagagcgg cgcgaacctg atcacgatcc tgggtggtca cacgctggac
     2701 ctggcgatcc tggtactcgg cgcaatcgac agcctcgatg cgctcaccac cctgcagcat
     2761 ccgacggtca cgcttaccga caccggcgag cagatccgac gcaccgcacc cgatcacctg
     2821 ctggtacagg cgcgcatgag cagcggctgt gccctggccg tcgaagtcgc cggtgaccga
     2881 ccacccgata cacccttcac tttcgaagtc gttgggactg agggacgaat ccttctggcc
     2941 ggcggtcacc caaacggctt tcaggctggt cgactcaccc tcagcctcga cggcgaacgt
     3001 caatgggtcg acgagccgcc cgacacgcta ccggccgctg cggtcaatgt ggcggcgatg
     3061 tatcgcgcgt tgtcccgcga cgtcactttc ggtgaacaca cggcgcccga cttcgaccac
     3121 gccttgcgat tgacccatct gatggatgac gtcgtgcagt cagccggcag tggccaccgc
     3181 ctgacacgta aggactggcc cgccggcgga tcttgaacgc aggacggcct cgacgaaccc
     3241 gcacgcttgc agccagtcgg tatgccgaga ccctatggtg cgggcatgtt ttggcggacc
     3301 tcgagcgcga ggcacatttc ggcgagttca tcaggcaccc cgagcgaccg gccggtgtcg
     3361 cgctcgatgc ggtgcagacg gtgccggaca gtgtttcggt ggacatgcag ctttgcggcg
     3421 gtattggtga ccgagcactt attggcaacc ccgccctgaa ggtgtcgaag aggacatcgt
     3481 tttcctcagt tggcaggtca tcccgactgt ggcggtgtac gaatcacgaa gagacagctg
     3541 gatgagcggc aaaccgcggc gcgtgctgaa gacgctcgaa ggcctagtcg ccgctgtcgt
     3601 tgacggtgtg accggggttg acccatgagg ccgccgcgcc gatcaccatc atcaccgcgg
     3661 cggcggtgaa caccacgact aacccggtgt ggaacggccc cgagatcaac tgggggaaga
     3721 attgttttcc ggtcagcacg gccgcgttga ctccgcgctg ttgcaggact ccggaggagc
     3781 ccagcagttc ggcgatcggg ttgtaaccca agaacgccgc gaacagactt tcggccggag
     3841 gcaggttggc cacgtcgtgg gccaccgtcg cgggtacgcc ttgctgctgc agtccggcga
     3901 ggagcgtggt gggcagcgag tgggccaggc cggcgatcat cagcgagaag aaaataccga
     3961 tcgataatga catgccggca ttggtgaccg tcgcacggat acccgacgcg gcgccgcgct
     4021 ccgcggccgg cacgctggac atgatcgccg cggtgttggg tgacgtgaag atgccgccgc
     4081 ccacgccgtt gaggaagatc agcacggcga acaacctgta gtcgaaatcc accggtatca
     4141 gcgccagggc cacgaatgtc atcgccatca gcatcagacc gccgatgacg aacggccggg
     4201 agccgaagcg gtccgatagg gcgcccgaca gcggacctgc cagcaggaag ccgagggtca
     4261 tcggcagcat gtagatgccc gaccacagcg gggtggattc aaagctgtag ccgtgcaacg
     4321 gtagccagat gccctgcagc cagatgatca gcatgaactg caggccgcca cgccccacgg
     4381 aggccatcag cccggcaagg ttgcccagcg cgaagagctt ggaccggaac agcctgaagt
     4441 cgagcatggg agcgggtgcg cgcgactcga caaagtacaa cgcgaccagt gcgaccacgg
     4501 ccagcgtgat cgaaccgagg acccaggggt tggtccaacc ggtgggagaa ccgcggtagg
     4561 gctggatccc gtaggtgatt gctgccagca gtgcggtcag gcccccgccg aaaagcactg
     4621 tgccgggcca atcgaggcgg gcgggagtcc ggacgccgac ttcacgcagc gagcggaaac
     4681 tccacaccgt cccgccca
//
LOCUS       DASRJA010000027         9367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1650686,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000027 DASRJA010000000
VERSION     DASRJA010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650686"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VFC01_01295"
     CDS             complement(<1..639)
                     /locus_tag="VFC01_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 family protein"
                     /protein_id="HZN78310.1"
                     /translation="MTDRGTKVDDDNAGLSAADLLELNRDLQGSRAIRLLATTNLSLY
                     ATLMERHLSDGAVAETELVVRLERDLEGLDDPQSGLALIKSWAAQGWLHRIVDERHDH
                     NVCYLTQDARRALDFLRGMRRQDTIATGGSITGIAARLKQVAVKVDNDPARIRKHIAA
                     EIAARHVELDQLDKNDGPTRLPSDLTDCYDEARAIALQMERLITDIGQYGTMI"
     gene            1038..2126
                     /locus_tag="VFC01_01300"
     CDS             1038..2126
                     /locus_tag="VFC01_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZN78311.1"
                     /translation="MAEHPTPVVPGSAVGIIPNPMSGRDIRRVVAQASVFPNTEKTSM
                     VLRIIRAVGALGVERVLLSTDTFGIAAGVLREVRRLRAGPPSTRVRLPEIDFLTLPQP
                     TGSATDTTEYARMMAQRCSAAVALLGGDGTMRAAAPGLGDTPMLALSTGTNNVFPIMI
                     EATVAGMALGLIATRRVHAPTARAKVLHVDVARSDGTRRHEIALVDVCVNTIGEIGAR
                     AVWKTDTLRELYCTFAEPDAIGLSSIPGRIMPTGRAAPNGVAVRMGPNGRSSHNVLAP
                     IAPGLLELIGLDGCEPLPPGITRQVGLARGTLALDGERELEFGPGDRISVTLAADGPR
                     VIDVPAALASECSCCSTVSMKSIAEEIR"
     gene            2123..3139
                     /locus_tag="VFC01_01305"
     CDS             2123..3139
                     /locus_tag="VFC01_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZN78312.1"
                     /translation="MTQTLSSLGADAPDASGLNETQLLDVYRTMRSIRAFEDRVHEEF
                     ATGDIPGFVHLYAGEEASATGVCAHLDPDRDTIASTHRGHGHCIAKGVDIKPMMAEIF
                     GRRTGSCRGKGGSMHIADLSKGMLGANGIVGGGPPLICGTALAAKLKGDGGVGVAFFG
                     DGASNQGTTLEAMNLASVWNLPAIFVAENNGYAEATSSTWSVAADNIADRAAGFGMPG
                     AIVDGFDFFAVYEAAGEAIKRARQGGGPTLLEVKFIRYYGHFEGDQQKYRGPNEVANA
                     RENLDCLKRFRLRVVESGRLTDAQLDEVDSQVTAFIDEAVAEAKAAPLPTPEDLLTDV
                     YVKY"
     gene            3170..4186
                     /locus_tag="VFC01_01310"
     CDS             3170..4186
                     /locus_tag="VFC01_01310"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZN78313.1"
                     /translation="MATRKLSYREAINEALRQEMERDPSVIVMGEDNAGGAGAPGEDD
                     AWGGVLGVTKGLYERFPGRVLDTPITESAFIGAAIGAATRGMRPVAELMFIDFAGVCL
                     DQIMNQAAKFRYMFGGKAVTPVVIRTMYGAGLRAAAQHSQGLYPVFTHLPGLKVVLPS
                     SPYEAKGLLIQSIRDDDPVIFCEHKAMYDMTGDVPEESYTIPFGEANVVREGDDVTIV
                     AMGRMVSLAEQAAVELESSGVSAEVIDPRTASPLDLDTILESVEKTGRLVVVDEASPR
                     CNLATDISAQVVSEAFGDLRAPIKMVSPPHVPVPFAQPLEDLYIPSVQDIVNSAKTVT
                     EWSQ"
     gene            4192..5325
                     /locus_tag="VFC01_01315"
     CDS             4192..5325
                     /locus_tag="VFC01_01315"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin dehydrogenase
                     dihydrolipoyllysine-residue acetyltransferase subunit"
                     /protein_id="HZN78314.1"
                     /translation="MVDNRIERITMPKWGLSMESGKVTDWLVAVGDELTEGADVCEID
                     TDKIAGELESTWTGVVRALVVGVNESVPVGGTIAIVAPAEVPQEDIDAVVAEARAQLE
                     SGAIEDVSGPKVLTVDVEGRQLSYAVLGDTESGNDPVLFVHGYGGDKNSWLFVQEPIA
                     AGRATYALDLPGHGASSKDVGDGTIQTLAQAVLGFLDTVGLNRVHLVGHSMGGAVITL
                     LAADPAATGKVASLTLISPVGFGPEINAGYLRGFAEAESRRDLKPHLLALFADPGLVN
                     RQLIDDLLKYKRLDGVDQALRSLVQNLVEGDKSAVDLTGELASFGGPAVAVWGREDKI
                     VPAANAAGLGNAATVHIVDGVGHMAHMEKPQAVVAAIREALGA"
     gene            5413..6261
                     /locus_tag="VFC01_01320"
     CDS             5413..6261
                     /locus_tag="VFC01_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN78315.1"
                     /translation="MRRAEAEMELAAIDGIEVEYELRGSGEPVVVIHWGVSARWAEPL
                     LAEPILAERYRVLRYHRVGFGGSSRVEGPISMADHAEHCRLLLRQLGIERAHVVGHSS
                     SALIALQLALDAPDAVQSLVLMDAARPAPATEAQAAFVREVAEAAIGRYRAGDVAGAV
                     DIWARGVFGAGYRASLDRGLPGVFEEILACADTFFAQELPAVQQWSFAETDARRIAQP
                     VLAVLGENSAPTFRERRDLLLAWLPSVEPFDLPGATHLLHVERPHEMAEALSSFFARH
                     PLEPSS"
     gene            complement(6479..6889)
                     /locus_tag="VFC01_01325"
     CDS             complement(6479..6889)
                     /locus_tag="VFC01_01325"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HZN78316.1"
                     /translation="MGVDSEVLEFRAQGYGRHVMDVRLLRFGSIEVDGRTYEHDIVID
                     RGAVRKRSKKPSKPYRDKFGHTPLSADEELPWGGPRLIIGTGAHGSLPIMPEVVAEAA
                     RRNIEVVAVPTEQACRLIAGLKGQEVRAVLHVTC"
     gene            complement(7098..7259)
                     /locus_tag="VFC01_01330"
     CDS             complement(7098..7259)
                     /locus_tag="VFC01_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78317.1"
                     /translation="MDVMLGGVVQNLQRHGAALERVHVDPQWSKAMSLLISNGLRHGF
                     TLGWDIGGT"
     gene            complement(7360..8523)
                     /gene="ilvA"
                     /locus_tag="VFC01_01335"
     CDS             complement(7360..8523)
                     /gene="ilvA"
                     /locus_tag="VFC01_01335"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HZN78318.1"
                     /translation="MRRTPVIASRVLSDRTGEQVWLKCENLQRTGSFKPRGAYNRIAN
                     LSDEDRARGVVAASAGNHAQGVAWAATELGIASTVFMPVTVPLPKLVATKAYGAQVHL
                     VGETIDDALVAAREYSEQHGTVLIHPFDHADIVAGQATVGLEILNQIRNVDTIVVPTG
                     GGGLVAGIAAAAHYLAPQVRVIGVQAANAAAWPGSLAAGQPVRAESMSTMADGIAVAM
                     PGVIPFGHVRAFADSVRTVSEDALSRALLLCMERAKLIVEPAGAAAVAALMTSAPELG
                     LNGPVCAVLSGGNIDPLVLTHVITHGLRAAGRYLAVKVTIPDRPGGLSTLLAVVSESG
                     ASVLDVVHSRTAPKLALDEVEVMLTVETRGPAHREEVLAALGSAGFIVSVEDN"
     gene            complement(9027..>9367)
                     /locus_tag="VFC01_01340"
     CDS             complement(9027..>9367)
                     /locus_tag="VFC01_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZN78319.1"
                     /translation="SDSEIQRRVITQAKTTEQRAWLAEVLSVALVQSWLASSTTCSAC
                     GNRLDELPLRMGQWSCPTCSVTHDRDHNAAKVILAAGRAERLNACGDHARPQPVAAVD
                     DETGSTPTAA"
BASE COUNT         1575 a         3044 c         3114 g         1634 t
ORIGIN      
        1 gatcatggtg ccgtattggc cgatgtcggt gatcaggcgt tccatctgca gggcgatggc
       61 gcgggcctcg tcgtagcagt cggtaaggtc agatgggagc cgggttggtc cgtcgttctt
      121 gtcgagttgg tcgagttcaa catggcgggc ggcgatttcg gctgcgatgt gcttgcggat
      181 gcgggcggga tcgttgtcga ccttgacggc gacctgcttg aggcgcgcgg cgatgccggt
      241 gatggagccg ccggtggcga tggtgtcctg gcggcgcata ccgcgcagga aatccagcgc
      301 ccggcgggcg tcctgggtga ggtagcagac gttgtggtcg tggcgttcgt cgacgatgcg
      361 gtgcagccaa ccctgggcgg cccaggactt gatcagggcg aggccggatt gggggtcatc
      421 gaggccctcg aggtcgcgtt ccagccgcac gacgagctcg gtctcggcga cggcaccgtc
      481 ggacaggtgg cgctccatga gcgtcgcata gaggctcagg ttggtggtgg cgagcagacg
      541 aatcgcccgg gagccctgca ggtcgcgatt cagctcgagc aggtcagcgg ccgacagtcc
      601 agcgttgtcg tcgtccacct tcgttcctcg atctgtcatt ggtcagacgc cccaacataa
      661 gcgacggcgc cgacatgtct gcgcgggaca gggccgtgtc ggcggcgatt tccgtcacca
      721 ggacggtttg ccgacgtcag cggaatgacc gcgctcccaa ggcattgcgc cgactcggcg
      781 taaggctggt gccactgtga atcgcgggac tcatggggct tctgggcttg tttcagctgc
      841 gtcgcgactg gaatggaacg gcggggcgga gacgacttgc cgtccggcgc cccactgccg
      901 aggggccaga cgtcaccacg gccaacttgc atgcaactcc ctgcaacgct tgacacttgt
      961 gatcggcgtc acatactcgc tgtagtgagc cgaccgaccg tacgagccga tcgaccgatg
     1021 acgtgcagga ggaaacggtg gccgaacatc cgactcctgt ggtccccggc agtgctgttg
     1081 gaattattcc gaatccgatg tcgggcagag atattcgacg cgtcgtcgcg caagcgtcgg
     1141 tcttcccgaa caccgaaaag acatcgatgg tattgcggat catccgtgcg gtcggggcgc
     1201 tgggcgtgga gcgggttctg ttgtccaccg ataccttcgg aatagcggcg ggtgtgctgc
     1261 gagaggttcg ccggcttcgc gctggcccgc cgtcaacccg cgtgcggctg cccgagatcg
     1321 acttcctgac cctgccgcaa cctaccgggt ccgcaacgga caccacggag tatgcccgaa
     1381 tgatggccca gcgatgctcg gccgcggtgg cgctgctcgg cggtgacggc actatgcgag
     1441 cggccgcgcc cggactcggt gacaccccca tgcttgcgct gagcaccggc accaacaacg
     1501 tgtttccgat catgatcgag gccaccgtcg ccggcatggc gttgggactg atcgcgaccc
     1561 gtcgtgtgca cgccccgacg gcgcgcgcga aggtcctaca cgtcgacgtc gccaggtccg
     1621 acggcacgcg ccgccacgag atcgccctgg tcgacgtgtg cgttaacacg atcggcgaga
     1681 tcggcgcgcg agcggtctgg aagacggaca cgcttcgtga gctgtactgc acctttgccg
     1741 aaccggacgc gatcgggttg tcgtccattc ccgggcggat catgccgacg ggtcgtgccg
     1801 ctccgaacgg tgtcgctgtc cggatgggcc ccaacggtcg ctcgtcgcac aacgtgctcg
     1861 cgccaatcgc accgggactg ctggagctga tcgggctcga tggctgcgag ccgctcccac
     1921 ccggtatcac ccgccaagtc gggctggcac ggggcacgct agcgctcgac ggcgaacgcg
     1981 agctggagtt cggtccaggc gatcggatca gcgtcacgct cgccgccgac ggtccccgcg
     2041 tgatcgatgt tcccgcagcg ctcgcaagcg aatgttcttg ctgctcaacc gtatcgatga
     2101 agtcaatcgc agaggagatt cgatgaccca aacactgtcc tcgctcggcg cggacgcacc
     2161 cgatgcgtcc ggcctcaacg agacccagct gctcgacgta taccgcacca tgcgatccat
     2221 ccgcgccttc gaggaccgcg tgcacgagga gttcgcgacc ggcgacatcc ccggcttcgt
     2281 gcacttgtat gcaggagagg aggcgtcggc gaccggagtg tgcgcacacc tcgaccccga
     2341 tcgcgacacg atcgcgtcca cgcaccgcgg gcacggccac tgcatcgcca aaggcgtcga
     2401 catcaagccg atgatggccg agatcttcgg aaggcggacg ggatcgtgtc gcggcaaggg
     2461 cggctcgatg cacatagccg acctgtccaa gggcatgctc ggcgcgaacg gcatcgtggg
     2521 cggtggaccg ccgctgatat gtggcaccgc gttggccgcg aagctgaagg gcgacggcgg
     2581 tgtcggtgtc gcgttcttcg gcgacggagc ctctaatcag ggcacgactc tggaggcaat
     2641 gaacctggcg tcggtgtgga acttgcccgc gatcttcgtc gcggagaaca acggctacgc
     2701 cgaggcgacg agctccacct ggtcggtggc ggccgacaac atcgccgatc gcgcagcagg
     2761 tttcggtatg ccaggtgcca tcgtcgacgg tttcgacttc ttcgccgtgt acgaggccgc
     2821 cggggaggcg atcaaacgtg cccggcaggg cggcgggcca accctgctcg aagtcaagtt
     2881 cattcggtac tacggccact tcgaaggcga ccagcagaag taccgtggcc cgaacgaagt
     2941 cgcgaacgcc agggaaaacc tcgactgcct caagcgattc cggctccggg tcgtcgaatc
     3001 cggacggctc acagatgctc aactcgacga ggtggacagc caggtcaccg cgttcatcga
     3061 tgaggccgtc gccgaggcga aggcagcacc actgcccacg ccagaggacc tgctcaccga
     3121 cgtctacgtc aagtactgat ccgggtgacc cgaaggagaa cgacgaatca tggcaacacg
     3181 caagctcagt tatcgagaag caatcaacga agcgctccgc caagagatgg agcgcgaccc
     3241 gtcggtcatc gtgatgggtg aggacaatgc cggcggtgcg ggagcgccgg gagaggacga
     3301 cgcctggggc ggagtgctcg gcgttaccaa gggcctgtac gagaggtttc ccggtcgggt
     3361 gctcgacacg cccatcaccg aatccgcgtt catcggcgcc gcgatcggcg ccgcgacccg
     3421 gggcatgcgc cccgtcgcgg agttgatgtt catcgacttc gccggcgtct gcctggacca
     3481 gatcatgaac caggccgcga agttccggta catgttcggt ggcaaggcgg ttacccccgt
     3541 tgtgatccgc accatgtatg gggcgggact gcgtgcggca gcgcagcatt cgcaggggct
     3601 ctacccggtc ttcacccacc tgcccgggct gaaggtggtg ctgccgtcca gtccgtacga
     3661 agcgaaaggc cttctcatac agtcgatccg ggacgacgac ccagtgatct tctgcgagca
     3721 caaggcgatg tacgacatga ccggtgatgt gcctgaggag agttacacca tcccgtttgg
     3781 tgaggcgaac gtggttcggg aaggcgacga tgtcaccatc gtcgcgatgg gccggatggt
     3841 gtcgctcgcc gagcaggccg ccgtcgagct cgaatcgtcc ggtgtttcag ctgaagtcat
     3901 cgatccccga acggccagcc cgctggacct ggacacgatc ctggagtcgg tggagaaaac
     3961 cggccggctc gtcgtcgtgg acgaggcgag cccgcggtgc aacctggcga ctgatatctc
     4021 cgcacaggtt gtgtccgagg ccttcggcga cctgcgggcg ccgatcaaga tggttagccc
     4081 gccgcacgtg ccggtgccgt tcgcccagcc gctcgaagat ctgtacatcc cgtcggtgca
     4141 ggacatcgtc aactcggcca agaccgtgac cgagtggagc cagtgatctc agtggtagac
     4201 aatcggattg aacggatcac gatgcctaag tggggcctgt ccatggagtc gggcaaggtc
     4261 accgactggc tcgtggccgt cggtgatgag ctgaccgagg gtgccgatgt gtgcgagatc
     4321 gacaccgaca agatcgccgg ggaactggag tcgacgtgga ctggtgtggt ccgcgcattg
     4381 gtcgtcggcg tgaacgagag tgtcccggtg ggcgggacga tcgcaatcgt cgcgcccgcc
     4441 gaggtgccgc aggaggacat cgatgcggtc gtcgccgagg cgcgggccca gctcgaatcc
     4501 ggtgcgatcg aggacgtttc aggaccaaaa gtcctcacgg tggatgtcga gggccggcag
     4561 ctgtcgtatg ccgtgctcgg cgacaccgag tccgggaacg atccggtgct gttcgtgcat
     4621 ggctacggcg gtgacaagaa ctcatggctg ttcgtacagg agcccatcgc cgccggccgc
     4681 gccacatacg cgctcgacct gccggggcac ggggcgtcgt cgaaggatgt cggggacggg
     4741 accatccaga ccctcgcaca agccgtcctc ggcttcctcg acacagtcgg cctcaacagg
     4801 gtccatctgg tcggccactc gatgggcggt gcggtcatca ccctgctagc ggccgatccg
     4861 gccgccaccg gcaaggtcgc ctcgttgacg ctcatctcgc cggtcggttt cggacccgag
     4921 atcaacgccg gctacctgcg cgggttcgcc gaagcggagt cgcgacgcga cctgaaaccg
     4981 cacctgcttg cactgttcgc cgatccggga ttggtcaacc ggcagctcat cgacgatctg
     5041 ctcaagtaca agcgcctcga cggtgtggat caggcactcc gatcgcttgt ccagaacctc
     5101 gtcgagggcg acaagtccgc ggtcgacctg acgggcgaac tggcctcttt cgggggaccc
     5161 gccgttgcgg tctggggccg cgaagacaag atcgtgcctg ccgcgaacgc agccggtctc
     5221 ggcaatgcgg ccacggtgca catcgtcgac ggcgtgggac acatggcgca catggagaag
     5281 ccgcaggcgg ttgtcgccgc gatccgagaa gcactcggtg cgtgaggtat tgcgagcgca
     5341 gatgacggca gcaccagaag gacgcaggtt actccggctg gaccttttcc cggtgtgaca
     5401 acgcatgtct gaatgaggcg tgcggaggcg gagatggagc tggcggcgat cgacggcatc
     5461 gaggtcgagt acgagctgcg gggctccggg gagcccgtcg tcgtgatcca ctggggcgtc
     5521 agcgcaaggt gggccgagcc actgctcgcg gagccgatcc ttgccgagcg ctaccgggtg
     5581 ttgcggtacc accgtgtcgg tttcggaggc agcagcaggg tcgaggggcc gatcagcatg
     5641 gccgatcacg ccgagcactg ccggctgctg ctgcggcagc ttggcatcga acgcgcgcac
     5701 gtcgtcggtc actcctctag cgccctaatc gcgctgcaac tcgcgctcga cgctccagac
     5761 gcagtgcaga gcttggtgct catggatgcg gcgcggcctg ctcccgcaac tgaggctcag
     5821 gcggccttcg ttcgcgaggt ggcggaagcg gcgatcggcc ggtatcgcgc gggcgacgtg
     5881 gccggggcgg tggacatctg ggcccgggga gtcttcggag cgggctaccg cgcctcgctc
     5941 gatcggggtc tgcccggcgt tttcgaggag atcctcgcct gtgcggacac attcttcgcc
     6001 caggagctgc cggctgtgca gcagtggtcg ttcgccgaga ccgatgcgcg ccgcatcgca
     6061 caacccgtcc ttgccgtcct cggcgagaac agcgctccga cattccgtga gcggcgggac
     6121 ctcctgcttg cctggctgcc gagtgtcgag ccgttcgacc tccccggcgc cacgcacctt
     6181 cttcacgtcg agcgcccgca cgagatggcc gaggcgctgt cctcgttctt cgcccgacac
     6241 cccctcgaac cttcgagctg agatcgacgt cgcctcagcc gctccccacc tcactgacgg
     6301 gacgaatgcg acggattcaa atacgcgggc gaccggtaaa ggtttctagc atcagcctgt
     6361 gccgcagcga ttttcaacga ccgtggtcca aggccgataa attcctcgct cctgagcggt
     6421 caagccggcc tcccagagcg gggctgctga cgatccggct ccgcttcaga tcgtcgggtc
     6481 agcaggtgac gtgaagtacc gcgcggactt cttgtccctt taggcccgcg atcagccggc
     6541 aggcctgttc cgtcgggaca gcgacaacct caatgtttcg gcgtgcggcc tccgccacca
     6601 cctcgggcat gatcggcaac gagccgtggg ccccggtgcc gatgatcaac cggggtccgc
     6661 cccacgggag ctcctcatcg gccgagagcg gagtgtgtcc gaacttgtcg cggtatggct
     6721 tggagggctt cttgcttcgt ttgcgcacgg cacctcggtc gatgacgatg tcgtgctcgt
     6781 acgttcgtcc gtcgacctcg atcgacccga acctgaggag tcgcacatcc atcacgtgcc
     6841 gcccataccc ttgcgcccgg aattccaaca cttcggaatc tacgcccatt gccagtgcgc
     6901 ggaagccggt gcgcccgact gcgccgaccc tggcgacgac ctcgtcgtgg acttcccgtc
     6961 ggagaccaga gagcctgcgg tgcaggctct ggctaggagc ggcgacgctc cccgagcggc
     7021 gactcagcgc tcggcagaca gacgccttta gcgaagccgc gccgctttag cctgccgcga
     7081 cgctcgccat gaagcaacta tgtgcctccg atgtcccagc caagggtgaa accgtggcgc
     7141 aggccgttag atatcaacag cgacatcgct tttgaccact gagggtcgac gtgcacgcgt
     7201 tccaacgcgg cgccgtgccg ctgcagattc tggaccaccc cgccaagcat gacgtccatg
     7261 gcgcctacat gatgcggcca gcgggcgggc gagtcctccc aggtctcccg gcgccccgct
     7321 ttgtgctcga gtcgatggcg gccgtcaggc agccgtgcgt taattgtcct cgacggagac
     7381 gatgaatccc gcgctgccca gggcggctaa tacctcctcc cggtgtgccg ggccccgggt
     7441 ctcgacggta agcatcacct caacttcgtc cagtgccagc ttgggtgctg tacgggagtg
     7501 caccacgtcg agaacgctgg ccccgctttc gctgaccacg gcgagcagcg tgctgagccc
     7561 gccggggcgg tccgggatgg tgaccttcac tgcgagatat cgtccggctg cgcgcaggcc
     7621 gtgggtgatc acgtgggtca acacgagcgg gtcgatattg ccacccgaca ggaccgcgca
     7681 caccgggccg ttcaggccga gttcgggtgc cgatgtcatc agcgctgcca ccgctgccgc
     7741 cccggccggt tcgacgatga gtttcgcacg ctccatgcac agcaacaacg cgcgcgacag
     7801 cgcgtcttcg ctgaccgtcc gaaccgaatc ggcgaatgcc cttacatggc caaatgggat
     7861 caccccgggc attgcgacgg cgatgccgtc agccatcgtc gacatcgact ccgcgcgcac
     7921 gggctgtccg gcggccaacg aacccggcca cgccgccgcg ttcgcggcct gcactccgat
     7981 cacccggacc tgcggcgcca gatagtgcgc cgccgcagca attccggcga ccagacctcc
     8041 gccgccggtc ggcaccacga tggtgtcgac gttccgaatc tggttcagga tctccagccc
     8101 gacggtggcc tggccggcga cgatgtcggc gtgatcgaac gggtggatca acaccgtgcc
     8161 gtgctgctcc gaatattcac gcgccgcgac cagcgcatcg tcgatcgttt cgccgaccag
     8221 atgaacttgc gcgccataag ccttggtggc caccagcttt gggagcggga cggtgaccgg
     8281 catgaacacc gtcgacgcga tccctagctc ggttgcggcc cacgcaactc cctgcgcatg
     8341 gttgcctgcg ctagccgcga ccacaccgcg agcccggtcc tcgtcactca ggttggcgat
     8401 ccggttatag gcgccccgcg gcttgaacga ccccgtgcgt tgcaggttct cgcacttgag
     8461 ccacacctgt tcgcccgtgc gatccgacag cacccgcgac gcgatcaccg gcgttcgtcg
     8521 catcaccggc ctcagccgat ccgccgccgc gttgatctga tcgagcgtga gcagctccac
     8581 agctagaccc gcagctcgtg aaccgccgcc ggatccccca cggggtcagc tggacaagcg
     8641 acgccccgcc gcgtgaatgc ggtggacggc cggagcgcat caaggtgggc gtcagtggcg
     8701 atgatgttgt cggcgttcat gtttggcctc gcttttgccg tcgagccgaa aggtgcaatc
     8761 gcgctctgcg tggtcaccac ctctgctcgt cccaatccac gcccgatgcg cggcgccgtg
     8821 aatggagggc accgatgtcg ggcgcagctc cctgcacggc cgtcgtcgac cagttcaagc
     8881 atcgcccggt gtcctttgaa tcgcccgcac acggattcgt cggatgatca gttagatgct
     8941 cgcactcctt gaccacacct gggggagcaa tacggcttgt tgggtttacg tgctcctcgg
     9001 actgggagtc cgaggattcc cggcggctat gccgctgttg gggtgcttcc tgtttcatcg
     9061 tccaccgccg caacgggttg cggtcttgca tggtctccac aagcgtttag tctctccgcc
     9121 cgcccggcgg cgagaatgac tttggcggca ttgtggtctc tgtcgtgggt gaccgagcaa
     9181 gtcgggcacg accattggcc catccgcaat ggcagctcgt cgagccggtt cccgcacgca
     9241 gagcacgtcg tgctcgacgc tagccacgac tgcaccaacg ccacactgag cacctcggcc
     9301 agccacgcgc gttgctcggt ggtcttggcc tgggtgatga ctcggcgctg aatctctgag
     9361 tccgaca
//
LOCUS       DASRJA010000028         4448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1947452,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000028 DASRJA010000000
VERSION     DASRJA010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947452"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VFC01_01345"
     CDS             <1..1365
                     /locus_tag="VFC01_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZN78320.1"
                     /translation="DPNENLYTLDYYLKLAEQIVDAGAHVLAIKDMAGLLRPQAAHQL
                     ITALRSRFDLPVHVHTHDTPGGQLATYLAAWHAGANAVDGASGPLAGTTSQPALSSIV
                     AAAAHTEYDTGLSLSAVCDLEPYWEALRKVYAPFESGIPGPTGRVYRHEIPGGQLSNL
                     RQQAIALGLGDRFEEIEANYAAADRVLGRLVKVTPSSKVVGDLALALVGAGVSADEFA
                     ADPAKFDIPDSVIGFLRGELGDPPGGWPEPLRTKALAGRPPAKPQQELSAEDETVLTQ
                     PGQKRQAALNRLLFPGPTKEFEAHRELYGDTSQLSANQFFYGLRQGEEHRVRLERGVE
                     LLIGLEAVSEPDERGMRTVMCILNGQLRPVVVRDRSIASDIPAAEKADRSNPDHIAAP
                     FAGVVTVNVEAGDQVDAGQTIATIEAMKMEAAITAPKAGTVGRVAVSATAQVEGGDLL
                     VVVS"
     gene            1362..1922
                     /gene="rsmD"
                     /locus_tag="VFC01_01350"
     CDS             1362..1922
                     /gene="rsmD"
                     /locus_tag="VFC01_01350"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZN78321.1"
                     /translation="MTRIVAGTFGGRRIAVPRTGTRPTTDRVRESLFNVLASRIDFEG
                     ASVLDLYAGSGALGLEALSRGAASAVFVESDQRAAAIIDKNIASLGVTAGVRRVPVAY
                     VLAGGAERPVDLVFADPPYNIPATEIEEMLAALVSGGWAAAGTVVVVERGTSGPELAW
                     PDAWQPWPSRRYGDTRIELAERTELP"
     gene            1937..2413
                     /gene="coaD"
                     /locus_tag="VFC01_01355"
     CDS             1937..2413
                     /gene="coaD"
                     /locus_tag="VFC01_01355"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZN78322.1"
                     /translation="MSGAVCPGSFDPITLGHVDIFERAAAQFDEVVVAVLVNPNKQGM
                     FSLDERIAMIEESTAHLPNLRVESGEGLVVDFVKARGLTAIVKGLRTGTDFEYELQMA
                     QMNKHIAGVDTFFVATTPRYSFVSSSLAKEVAMLGGDVSELLPEPVNVRLQAKLEG"
     gene            2498..3064
                     /locus_tag="VFC01_01360"
     CDS             2498..3064
                     /locus_tag="VFC01_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZN78323.1"
                     /translation="MVETFVESSDDVVKFLKGQHDLIKDMFDEVLSASEPKAREKAFV
                     ELRQLLAVHETAEEMVVHPRVRHESASGDEIVDARLKEEHKAKEMLSKLESMDIASPQ
                     FIKELTKFRDDVIDHAEHEENEEFTKLERNLTADDRERMAKAVKAAEAIAPTRPHAGV
                     ESAKMNFAVGPFASMLDRARDLIGAALR"
     gene            3403..4140
                     /gene="sepIVA"
                     /locus_tag="VFC01_01365"
     CDS             3403..4140
                     /gene="sepIVA"
                     /locus_tag="VFC01_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepIVA"
                     /protein_id="HZN78324.1"
                     /translation="MYRVFEALDELGAIVEEARGVPMTAGCVVPRGDVLELIDDIKDA
                     IPGELDDAQDVLDARDGMLREAKDHSDQMVGTATAEADSLINHSRAEADRLLADAKAQ
                     ADRMVAEARHHSERMVGEAREEANRISATAKREYEASAGRAKTEADRLIENGNLAYEK
                     AVQEGIKEQQRLVSQTEIVQTATAEATRLIDSAHAEADRLRGECDIYVDSKLAEFEDF
                     LNGTIRSVGRGRHQLRTAAGTHDYAAR"
     gene            4171..>4448
                     /locus_tag="VFC01_01370"
     CDS             4171..>4448
                     /locus_tag="VFC01_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HZN78325.1"
                     /translation="MATHANAAARRASRSPLVINISRLGRRPGSMMTYSETVPSPSRI
                     GLDLIGIDAGAPLTLDLRIESVSEGVLVTGNVAAPTTGECARCLTPITG"
BASE COUNT          808 a         1411 c         1533 g          696 t
ORIGIN      
        1 gacccgaacg aaaacctgta cacgctggat tattacctca agttggccga gcagatcgtc
       61 gatgcgggcg cgcacgtgtt ggcgatcaag gacatggcgg gcctgctacg cccgcaggcc
      121 gctcatcagc tgatcaccgc gctgcgcagc cggttcgacc tgccggtgca cgtgcacacc
      181 cacgacactc cgggcgggca gctggccacc tatctggccg cctggcacgc aggcgccaac
      241 gccgtcgacg gtgcctctgg gccgttggcg ggcaccacca gccagcccgc gctgtcctcg
      301 atcgttgccg ccgctgcgca cacggaatac gacaccgggc tgtcgttgtc ggcggtatgc
      361 gatttggagc cgtactggga ggcgctgcga aaggtgtacg cgcccttcga atctgggatt
      421 cctggaccga cggggcgggt gtatcgccac gagatccccg gcggtcagtt gtccaatctg
      481 cgtcagcagg cgatcgcgct gggtctcggt gaccgttttg aggagatcga ggccaactac
      541 gcggccgccg accgggtgct gggccgcctg gtgaaagtca ccccgtcgag caaggtggtc
      601 ggcgacctcg cgctggccct ggtcggggcg ggtgtcagcg ccgacgaatt cgctgctgac
      661 ccagcaaaat tcgatatccc cgattcagtg atcggcttct tgcgcggtga actcggcgac
      721 ccgccgggcg gctggcccga accgctgcgc accaaggcgc tcgccggacg cccacccgcc
      781 aagccgcagc aggaactatc tgccgaagac gagacggtgt tgacgcagcc gggccagaaa
      841 cgacaggccg cgctgaaccg cctgctgttc cctggtccga caaaggaatt cgaggcccac
      901 cgcgagttgt acggcgacac gtcgcagttg agcgccaatc agttcttcta cgggctgcgc
      961 caaggcgaag aacaccgagt caggctggag cgcggtgttg agctgctgat cgggctggaa
     1021 gcggtctccg aacccgacga gcgcggcatg cgcacggtga tgtgcatcct caacggtcag
     1081 ctgcgaccgg ttgtggtgcg tgatcgcagc atcgccagcg acataccggc cgccgagaag
     1141 gccgaccggt ccaaccccga ccacatcgcc gcgccgttcg cgggcgtggt caccgtcaat
     1201 gtcgaggccg gcgatcaggt cgacgccgga cagaccatcg ccaccatcga ggcgatgaag
     1261 atggaagccg cgatcaccgc acccaaggcc ggcacggtcg gccgcgtcgc tgtctcggcc
     1321 accgcgcagg tcgagggcgg cgatctgctg gtggtggtga gctgacccgc atcgtcgccg
     1381 gcacgttcgg cgggcgacga atcgcggtgc cgcgcacagg aactcggccc accaccgatc
     1441 gagtgcgtga gtcgctgttc aacgtgctcg catcgcgcat cgactttgaa ggggcttcgg
     1501 tgcttgacct gtacgcgggc tcgggggcgc ttggcctgga agcgctgtcg cgcggagccg
     1561 catcggcagt gttcgtcgaa tccgaccaac gggccgccgc catcatcgac aagaacattg
     1621 cgtcgttggg cgttacggcg ggagtgcggc gtgtcccggt cgcctatgtg ctggccggcg
     1681 gtgcggagcg gccggtggat ctggtgttcg ccgatccgcc gtataacatc ccggccaccg
     1741 agatcgagga gatgctcgcc gcattggtca gcggaggctg ggcggcggca gggacggtcg
     1801 tcgtcgtgga acgcggcacg tccgggccgg agttggcctg gccggacgct tggcagccat
     1861 ggccgtcacg gcggtacggc gacacccgca tcgagctcgc cgagcggact gagctgccct
     1921 gatagcgtcg tcacccatga gcggcgcggt atgcccggga tcattcgatc cgataaccct
     1981 cggccacgtc gacatcttcg agcgcgcggc cgcccaattc gacgaggtcg tggtggcggt
     2041 gctggtcaac ccgaacaagc agggcatgtt cagcctcgat gagcgcatcg cgatgatcga
     2101 ggaatcgaca gcgcatctgc cgaacctgcg cgtcgaatcc ggtgagggcc tggtcgtcga
     2161 cttcgtgaag gcgcgcggac tgaccgcgat cgtcaagggt ctgcggacgg gcaccgactt
     2221 cgagtacgag ctgcagatgg cgcagatgaa caagcacatc gccggcgtcg acaccttttt
     2281 cgtcgcgacg acaccgcggt attcgttcgt gtcgtcgtca ctggcaaaag aagtcgcgat
     2341 gctcggtggc gacgtgtctg agctgctgcc cgaacccgtc aacgtgcgcc tgcaggccaa
     2401 gctggagggt tagctctcgt ccttcgcggg taggcgttgg gtggcgcaca gcccatttcg
     2461 cgccccaacc aatccgagaa gtacggaggt gacggccatg gtcgagacat tcgttgagtc
     2521 gagcgatgac gtggtgaagt tcctgaaggg tcagcacgac ctgatcaagg acatgttcga
     2581 cgaagtcctc agcgcatcgg agccgaaggc ccgcgagaag gcgttcgtcg agcttcgaca
     2641 actgctggcg gtgcacgaga cagccgagga gatggtggtg catccgcggg tccggcacga
     2701 gtcggcttcg ggcgacgaga ttgtcgatgc ccgcctgaag gaggagcaca aggccaagga
     2761 aatgctctcg aagctcgaaa gcatggatat cgcttcgccg cagttcatca aggagctcac
     2821 caagttccgc gatgacgtca tcgaccacgc cgagcacgag gagaacgagg agttcaccaa
     2881 gctggagcgc aacctcacgg cagacgaccg cgagcggatg gccaaggcgg tcaaggcggc
     2941 cgaggcgatc gcgcccacgc gtcctcatgc gggagtcgag tcggccaaga tgaactttgc
     3001 cgtcgggccg ttcgcctcga tgctggaccg cgcccgcgat ctgatcggcg ccgcgctgcg
     3061 gtagtcggcc gaacggtttt gctgccgggc cgctcgtgtg gatgcgcgat cgggcccgcg
     3121 gcgttctcaa acaggccagc cgcagagccg tcggtgatgt gctgagggga actaaaatcg
     3181 cccgtcacgt cggatgtgcg ggcgattttg gttttgccga gcatcgtcgg cgaagcgctg
     3241 acctgctgta ttgacataat gtgcgggtag caattcagac gggagttgcg cctggagtca
     3301 cgctggggaa gccaaaggac gacacaccgg ccccggaagc taagtcacag aggtaacacc
     3361 aggcacactg gtaactgtca actacgcctg gagggtgttg ccgtgtaccg agtttttgaa
     3421 gcgctcgacg agttgggcgc gattgtggaa gaagcccgcg gcgtgccgat gacggccggc
     3481 tgcgtggtgc cccgcggcga cgtcctcgag ctgatcgacg acatcaagga cgcaataccc
     3541 ggcgaactcg acgacgccca ggacgtgctc gatgcgcgcg acgggatgct gcgcgaagcc
     3601 aaggaccact ccgaccagat ggtgggcacg gccaccgccg aagccgattc gctgatcaac
     3661 cactcgcgcg ccgaagccga ccggctgctg gccgacgcca aggcccaggc cgaccggatg
     3721 gtggccgagg cccgccacca cagcgaacgg atggtcggcg aggcccgcga ggaggcgaac
     3781 cgcatcagcg ccaccgccaa gcgggagtac gaggccagcg ccggacgcgc caagaccgag
     3841 gccgaccgcc tcatcgagaa cggcaacctg gcctacgaga aggccgtgca ggaaggcatc
     3901 aaggagcagc agcggctggt gtcgcagacg gagatcgtgc agaccgcgac ggccgaggcc
     3961 actcgcctga tcgactcggc gcacgccgag gccgatcgcc tacgcggtga gtgcgatatc
     4021 tacgtcgaca gcaagctcgc ggagttcgag gacttcctga acggaaccat taggtcggtg
     4081 ggtcgcggcc gtcatcagct gcgcactgcg gcgggcaccc acgactacgc cgcacggtag
     4141 cccgaggccg tcgccgtaag atcgacattt atggcgacgc atgcgaacgc agcggcgcgc
     4201 cgcgcctcac gatcgccgct cgtgatcaac atttcccggc tcggccggcg gcccgggtcg
     4261 atgatgacct actccgagac ggtgccgagc ccgtcacgca tcgggctcga cctgatcggg
     4321 atcgacgcag gcgcgccgct gacgctggat ctgcggatcg agtccgtgtc ggagggggtg
     4381 ctggtcaccg gtaacgtggc cgcgccgacg accggcgagt gtgcccgctg tctgaccccg
     4441 atcaccgg
//
LOCUS       DASRJA010000029         4775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_545852,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000029 DASRJA010000000
VERSION     DASRJA010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545852"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFC01_01375"
     CDS             <1..607
                     /locus_tag="VFC01_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HZN78326.1"
                     /translation="GSGRTLVGGNWAMTAPYIVNPGYFSPRAEWELFEASADRRWTDI
                     TRTQRVLGWQLIGTGMLPPDWATVSEVGHAVPTRPVTGGPITFGLDAARLPVRFAESC
                     DREDRALAGAMRPILTAPGEVPALRNLNGSAASSWQHPVALVAAAATEQGAGNPDVAV
                     ERLDAAAQLEQRSPTYYGAAWVALGRIMLTTSLLGECPVAG"
     gene            complement(663..2012)
                     /locus_tag="VFC01_01380"
     CDS             complement(663..2012)
                     /locus_tag="VFC01_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78327.1"
                     /translation="MEIVLGVSMTPTTVSIALVEGEKADGVIVERDVFDIAAVDGSAT
                     SSASDHVVAAVLGTQEGAIAAGHHLVSTGVTWTDHAQAAALREALIARGIEDVLLVSE
                     LHAAAALAQAVGRAVGYDKTALMFVERDTATLAVVETADGSVVKVLSQGLHSADTIAV
                     LTEMVTSLQAEKSTPQGMFVVGSNVDVTAVKAHLKDLVSVPVIAPEEPQVALARGAAL
                     AAANAPRFDASTVGLAYSLDPDDTTDSPLALADDVTAFLGPADALVDTADVASDSDAV
                     SSRSKPFLLVGSSLTAIFVAGMMALAIAIVINIRPTADQGSGEMTLRPEAVAPPTALQ
                     TAPPVAPQQVPRTAPAPAPAVPPAPIETARLKVLPAPPPRVVVRNVAPAPAAPPVAPP
                     PVVVPPPMPPPLIPWLPPILQPPPYYNPQLGPQWYPGPKPPWGRGGSRHGDGDDHGD"
     gene            complement(2436..3302)
                     /locus_tag="VFC01_01385"
     CDS             complement(2436..3302)
                     /locus_tag="VFC01_01385"
                     /inference="COORDINATES: protein motif:HMM:NF028206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZN78328.1"
                     /translation="MTNQSATVTVIGAGGKMGQRISNNLAKSDYRTLYCESSPHGREL
                     IAGLGRELTGTDEAVAQSDVVILAVPDVVLGKVSAAVVPSMKSGAILLTLDPAAAYAN
                     LLYQRDDIAYAVAHPCHPSVFLERTTKEEHADTFGGIAAPQEVVAAFENGNAGQRDLV
                     EGVVRVSYGPVLDVHWVTVKQLAVLEPTLVETVACMVGGLLKEALHETVHTVGVPEAA
                     AKAMLFGHVQVALANTLRGSNPFSDACLIAMDYGRESIVRDDWKKIFDDGELDKVITR
                     MLHLDQIARTPA"
     gene            complement(3299..4057)
                     /locus_tag="VFC01_01390"
     CDS             complement(3299..4057)
                     /locus_tag="VFC01_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN78329.1"
                     /translation="MAIGLSTYAFFWRKLSLHDMLKQTNELGCTVFQICDYPAVESLS
                     AAELADLRDTAADLGIELELGTRGVAPEHLHRYLDLCKQLGATLLRSMLQQTGDSVSM
                     LKETMPAFESQGVTVALETYEQVPTPELIRVIDAVGSDALGICLDPANCVAGLELPHD
                     VIDRAAPYVKNMHIKDFAFTRQAGWVGFTLAGCPLGEGLLDYDEMVAVVDPASRGINQ
                     IVEHWLPWQDDAETTCLFEAQWTQHSVNYLRSKS"
     gene            complement(4042..>4775)
                     /locus_tag="VFC01_01395"
     CDS             complement(4042..>4775)
                     /locus_tag="VFC01_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase family protein"
                     /protein_id="HZN78330.1"
                     /translation="LTIGISLKMYFGHTRTVEWCGAVARIAHSHPAVTEEHARLVVLP
                     SHPYLLEAGAVFASTPVRIGGQDLFWKDEGAFTGEVSGAQLRELGCDYVEIGHAERRR
                     IFGEDDKVIAAKTAAAFRNGLTPILCVGEQRRSTVEAAAAECVAQLHSSGPMVVAYEP
                     VWAIGADEPASPGYIAAVCRALRSHVSGPVIYGGSAKPGLLTQLGDGVDGLFLGRFAH
                     DPSAVAAILDEVVDLAEGGPRWRSA"
BASE COUNT          776 a         1595 c         1576 g          828 t
ORIGIN      
        1 cggctcgggg cgcacgctgg tcggcggaaa ctgggctatg acagcgccgt acatcgtcaa
       61 ccctgggtac ttcagtcctc gcgccgaatg ggaactcttc gaggcatcgg ccgaccggcg
      121 gtggacggac atcacccgca ctcagcgggt cctgggctgg cagctcatcg ggaccgggat
      181 gctgccgccc gactgggcca ccgtcagtga agtcggccat gccgtgccca cccgacccgt
      241 caccgggggc cccataacct tcggtctcga cgccgcgcgc ctgccggtcc ggttcgcaga
      301 atcctgcgat cgagaggacc gagccctcgc gggtgcgatg cgcccgatcc tcacagcacc
      361 gggtgaagtt cccgctttgc gcaacctgaa tgggtcggcc gccagcagtt ggcagcatcc
      421 cgtcgccttg gtcgccgccg cggccaccga acagggcgcc ggcaatcccg acgtcgccgt
      481 cgagcggctc gacgcggcgg cgcaactgga gcagcggtcc ccgacctatt acggcgccgc
      541 ctgggtggcg ctgggccgca tcatgctgac cacgtcgctg ctcggcgaat gcccggtggc
      601 cggctgagcg tttcgatcac cgcgatcgtc acgaatggcg atccggcgca gccaattcca
      661 gcctaatcac cgtggtcgtc gccgtccccg tgccgggagc cgccgcggcc ccacggcggc
      721 ttcggacccg ggtaccactg cgggcctagc tgcgggttgt agtagggggg tggctgaaga
      781 atcggtggga gccacgggat gagcggcggc ggcatcggtg gcggtaccac aacgggcggc
      841 ggagcgactg gcggcgccgc cggggcgggc gctacgttgc gcaccaccac tcgcggcggc
      901 ggggcgggca gaaccttcag tcgtgccgtc tcgatgggcg caggtggtac cgccggagcc
      961 ggcgcagggg ccgtcctggg cacttgctgc ggcgcgacgg gcggcgcggt ctgcaacgcg
     1021 gtaggcggcg ctacagcttc gggacgaagc gtcatctcgc ccgagccttg atccgcggtc
     1081 gggcggatat tgatcactat tgcgatggcc agagccatca ttcccgctac gaagatggcg
     1141 gtcagcgagc tgcctaccaa taggaacggc ttgctgcggc tcgacacggc gtcggagtcc
     1201 gaagccacgt cggccgtgtc cacgagtgcg tcggcggggc caaggaacgc ggtcacgtca
     1261 tcggccagcg ctagcgggga gtccgtggtg tcgtcgggat ccagggagta ggccaggccg
     1321 acggtggacg catcgaagcg cggcgcattc gctgctgcca gcgcagcgcc gcgcgccaac
     1381 gccacctggg gttcttcggg ggcgatgacg ggcaccgaga caaggtcctt caaatgtgcc
     1441 ttgaccgcgg tgacgtccac gttggagcca acgacgaaca tgccctgggg cgtggatttc
     1501 tcggcctgca ggctggtgac catttcggtc agcacggcga tcgtgtccgc actgtgcaga
     1561 ccttggctca gtaccttcac cacggagcca tcggcggttt cgacgaccgc cagcgtcgcg
     1621 gtgtcgcgct cgacgaacat cagtgcggtc ttgtcgtagc ccactgcccg gccgaccgcc
     1681 tgggcgagcg ccgcggccgc gtgcagctcc gagaccagca gcacatcttc gatgccgcgg
     1741 gcgatcaatg cctcccgcag tgcggccgct tgggcgtggt cggtccaggt caccccggtc
     1801 gacaccaaat ggtggccggc ggcgatcgcg ccctcctggg tacccaggac cgcggcgacg
     1861 acgtgatcgg atgcgctcga cgttgccgag ccgtcgacgg cggcgatgtc gaagacgtcg
     1921 cgctcgacaa tgacaccgtc ggccttttcg ccctcaacga gtgcaatgct gaccgtcgtg
     1981 ggtgtcatcg acacgccaag tacgatctcc actgcccctc caagacattt gctacgcata
     2041 ttcggctctt ggcggggagc gtgtcccacg caagctgtga acgtacttcc gcccgtcgcc
     2101 gctgaccact tccggtgggt catgtcgtcg tgggctcgcg cttgccctcc gataaaaaac
     2161 cctactcgct cagtcgaata ttgacgcgcg atgcggcgag attcttgaga ctttgccggg
     2221 cttccaccat cgcggtcgag gtcattcggc ggccgatgaa ttcggttgcg cggaagtgct
     2281 tttgagttct taggtgtgtg gttctaccgc ttcggtatta gctggcattt ggcattcgga
     2341 tccgaacagc gggaccccgt ttccgaaatc ccagaagcgc acggcgacgg tagaaaggat
     2401 ggtgctcgcc aaaggtccac tacggcgcga aacggctacg cgggtgtgcg cgcgatctgg
     2461 tcgaggtgca gcatccgcgt gatgaccttg tccagctcgc cgtcgtcgaa gatcttcttc
     2521 cagtcatcgc ggacgatgct ttcgcggcca tagtccatcg caatcaggca cgcatcggag
     2581 aacgggttcg acccgcgtag cgtattcgcc agcgccacct gaacgtgccc gaaaagcatt
     2641 gccttggcag cagcttcggg cacgcctacc gtgtgcaccg tctcgtgcag ggcctccttg
     2701 agcaggccgc cgaccatgca ggccaccgtc tcgaccagcg tcggctccag cacggcgagc
     2761 tgcttgaccg tcacccaatg cacatcgagc actgggccgt agctcactcg gaccacgccc
     2821 tcgacgaggt cgcgctgacc agcattcccg ttctcgaacg ccgcgacgac ctcctgcggc
     2881 gcggcgatac cgccgaacgt gtcggcgtgc tcttccttgg tggtgcgctc gaggaacacc
     2941 gaggggtggc aggggtgcgc gaccgcgtat gcgatgtcgt cgcgctggta cagcagattg
     3001 gcgtacgccg ccgccggatc cagcgtcagc aggatcgccc ccgacttcat cgacggcacg
     3061 acagctgccg acaccttgcc cagcaccacg tccggtacgg ccaggatgac gacatcgctc
     3121 tgagccaccg cctcgtcggt accggtcagc tcgcgtccca gaccggcaat cagctcacgt
     3181 ccgtgcggcg agctctcgca gtacagcgtc cggtagtcgc tcttggcgag attgttcgaa
     3241 atgcgttgtc ccattttgcc tccggcgccg ataacggtga cggtggctga ctggttggtc
     3301 atgacttgct cctcagatag ttgacactgt gttgtgtcca ttgggcttcg aacaggcagg
     3361 tcgtctcggc gtcgtcctgc caggggagcc agtgctccac gatctggttg attcctcgcg
     3421 acgctgggtc cacgacagcg accatctcgt cgtagtcgag cagtccttcc ccgagcgggc
     3481 agcccgcgag cgtgaagccg acccagccgg cctgccgggt gaacgcgaag tccttgatgt
     3541 gcatgttctt gacatacggc gcggcgcggt cgatgacatc gtgcggcagc tctaggcccg
     3601 cgacgcagtt cgccgggtcc aggcagatgc ccaacgcgtc ggagccgacg gcatcgatca
     3661 cgcgaatcag ctcgggtgtc ggaacttgct cgtaggtctc caaagcgact gtcacaccct
     3721 ggctttcgaa cgctggcatc gtctctttga gcatcgacac cgaatcgccg gtttgctgca
     3781 gcatcgaccg cagcagcgtc gcgccgagtt gcttgcagag gtcgaggtag cggtgcaggt
     3841 gctcgggcgc gacgcccctg gtgccgagct ccagctcgat gccgaggtct gccgcggtgt
     3901 cgcgcagatc ggccagttcg gccgcggaca gcgattccac ggccgggtag tcgcagatct
     3961 gaaagaccgt gcagcccaac tcattggtct gcttgagcat gtcgtgcagc gacagcttcc
     4021 gccagaagaa cgcgtaggtg ctcaggccga tcgccatcgt ggtcctccct ccgcgaggtc
     4081 gacgacctcg tcgagtatcg ccgcgaccgc cgacgggtca tgagcgaagc ggccgaggaa
     4141 caaaccgtcc accccgtcac ccagctgcgt caacagcccg ggcttggcgc tgccgccgta
     4201 gataacggga ccggacacat gtgaccgcag cgcccggcac accgcggcga tgtaaccggg
     4261 tgatgcgggc tcgtccgcgc cgatcgccca cactggttcg taggccacca ccatcgggcc
     4321 ggacgaatgc agctgcgcga cgcattccgc cgcggcggcc tcgaccgtcg agcggcgctg
     4381 ctcacctaca cacaggatcg gggtgagtcc gttgcggaac gccgccgctg ttttggccgc
     4441 gatgaccttg tcatcttcac cgaagatgcg ccgccgctcg gcgtgcccga tttcgacgta
     4501 gtcacaaccg agttcgcgca gttgcgcacc gctgacctct ccggtgaagg ccccctcgtc
     4561 cttccagaag aggtcctggc cgccgatgcg cactggcgtg gaagcgaaca cagcgcccgc
     4621 ttcgagcagg taggggtgcg acggcagcac caccaacctc gcgtgttcct cggttaccgc
     4681 cggatgcgag tgggcgatgc gggcgaccgc gccgcaccac tcgaccgtgc gggtgtgccc
     4741 gaagtacatc ttcaggctga tgccgatcgt cagag
//
LOCUS       DASRJA010000030         5851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3920644,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000030 DASRJA010000000
VERSION     DASRJA010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3920644"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /gene="arc"
                     /locus_tag="VFC01_01400"
     CDS             <1..937
                     /gene="arc"
                     /locus_tag="VFC01_01400"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HZN78331.1"
                     /translation="KTLIAKAVANSLAKKMAEVRGDDAREAKSYFLNIKGPELLNKFV
                     GETERHIRLIFQRAREKASEGTPVIVFFDEMDSIFRTRGTGVSSDVETTVVPQLLSEI
                     DGVEGLENVIVIGASNREDMIDPAILRPGRLDVKIKIERPDAEAAQDIYSKYLTEELP
                     IHTDDLAEFGGDRTVCIEAMIVKVVDRMYAEIDDNRFLEVTYANGDKEVMYFKDFNSG
                     AMIQNVVDRAKKNAIKSVLETGQPGLRIQHLLDSIVDEFAENEDLPNTTNPDDWARIS
                     GKKGERIVYIRTLVTGKSSSASRAIDTESNLGQYL"
     gene            1034..1729
                     /locus_tag="VFC01_01405"
     CDS             1034..1729
                     /locus_tag="VFC01_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78332.1"
                     /translation="MSDDVTVIDDVYGKRYGEMLLVTTTPAGPEATVYKTFPLNDCPV
                     ELWDKLDPQAIAADNGAVAALLNGPRYWLMSSIGKRSRETLERKSFGGIEMIRQATVK
                     LASMNPSPYSLNRVDRKAVFTFDAGREIYELVDPDGRRWVMQTWSQTVDPALSLSDLP
                     GLASRLSLPDDWTYRPRTPTSSLRVDTTSQDACVRQDDLANSYSLQTSGTRKPPKSQG
                     FGGFAARLVSRGG"
     gene            complement(1713..1958)
                     /locus_tag="VFC01_01410"
     CDS             complement(1713..1958)
                     /locus_tag="VFC01_01410"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN78333.1"
                     /translation="MLRTPAAPAFADEESYLTDLANNDFTGPTDVALDMGYQICTDLQ
                     HGVPQNNTVKAIYENTGDSIEVADAQYIYEAAVIHLC"
     gene            complement(1992..3134)
                     /locus_tag="VFC01_01415"
     CDS             complement(1992..3134)
                     /locus_tag="VFC01_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78334.1"
                     /translation="MRQVHEWFLQRGLPLVLTRRVRSRALIERSAPMVSGIGAVISLT
                     MMLAELTDGDPDFGYVIRLGILTGLLIAAPFALYLLHRTGTTLGEAGRRSAALLVMAI
                     FVFGLPFADSGFSGIAAAEAVGFAVVALLAIWLTYLGFGSIALWAFRFAWVQFGALGT
                     LMSRALPLLMITVVVYFTGELWQLSARLTRQRLWQTIGFLALVALIFVVTTIRDEVRA
                     LREDRAEQSDPAKLLEGTPLETAAGHQPVRTPLSLAEQVNVVAVMVVSQAIQVVFFTA
                     GLFAFFLALGIVAVPDDVAVLWSSEETCPVGEPPCPGTWFGIHVPVPQTIVHVSLFVA
                     VLSGLYFTVSTSVDPLYRERFFTPLIDDVAVSLAGRDAYLEMEGAR"
     gene            complement(3181..4323)
                     /locus_tag="VFC01_01420"
     CDS             complement(3181..4323)
                     /locus_tag="VFC01_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78335.1"
                     /translation="MIDVTQRRAADAWFLDRGLPAVLRPGALVRRLWPRSAPVLAGFA
                     VFMANSALVVAVTGKHTIDINGHPTRTEWFVLALLILVLPIAVLVGWLVSRIPTVPGR
                     TVAATVATTVAVAGGVLGGPSPRILVDLIFEAIVIAAILALTACGAGSILGWAVQMTT
                     SNLAAAGSMVTRALPLLLLTILVFFNSPVWLMAATVSRPRLWLALAFLTAIAAVFVVS
                     VSQDRVKPMLDSDEPAHDHSVRLAGTPFETMPDPASARTLSRVEQLNVMFVVAASLLS
                     QILIIAAMTSLLFLILGLILLSPELLAAWTRNGSSDGTVLGMTIPVPDSLIQITFFLG
                     ALTFMYISARTVSDGDYRTRFFDPLIDDLRLTLTARNRYRAALSAR"
     gene            complement(4316..4858)
                     /locus_tag="VFC01_01425"
     CDS             complement(4316..4858)
                     /locus_tag="VFC01_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78336.1"
                     /translation="MQIAQLAFGILIAAQVAGIADTNLVTTEINRLGAWAPLLGVFVY
                     GIGAMLYFGPPTSFLPWMLLMLLTAYAGQWVGNAVLGSYASGFGGGLTLIICALAISH
                     RPNTPPTVSLVLPGFWLLVPGSLGFMGVTQLLGTHSTAVFTATLISMMSIAVGVQTGL
                     LLWRAAIQATSAPNRRAFHD"
     gene            complement(4935..5495)
                     /locus_tag="VFC01_01430"
                     /pseudo
     CDS             complement(4935..5495)
                     /locus_tag="VFC01_01430"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
     gene            5602..>5851
                     /locus_tag="VFC01_01435"
     CDS             5602..>5851
                     /locus_tag="VFC01_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HZN78337.1"
                     /translation="MQRIIGTEVEYGISSPSDPTANPILTSTQAVLAYAAAAGIQRAK
                     RTRWDYEVESPLRDARGFDLSRSAGPPPVVDADEVGAAN"
BASE COUNT         1191 a         1837 c         1908 g          915 t
ORIGIN      
        1 caagacattg atcgccaagg ccgtggccaa ctcgctggcc aagaagatgg ctgaggttcg
       61 tggcgacgac gcccgggagg cgaagtcgta ctttctcaac atcaagggcc cggagctgct
      121 caacaagttc gtcggcgaga ccgagcggca catccggctg atcttccagc gcgcccgtga
      181 gaaggcgtcc gaaggcacgc cggtgatcgt gttcttcgac gagatggact cgatcttccg
      241 cacccgcggc accggtgtga gctcggacgt ggagaccacg gtggtgccgc agctgctgag
      301 tgagatcgac ggtgtcgagg gcttggagaa cgtcatcgtg atcggcgcct ccaaccgcga
      361 ggacatgatc gaccccgcca tcctgcggcc cggccgcctg gacgtgaaga tcaaaatcga
      421 gcgcccggat gccgaagcgg cacaggacat ctactccaag tacctcaccg aggaactgcc
      481 gatacacacc gacgatctgg ccgaattcgg tggcgaccgc acggtctgca tcgaggcgat
      541 gatcgtgaag gtcgtcgacc ggatgtacgc cgagatcgac gacaaccggt tcctggaagt
      601 cacctacgcc aacggtgaca aggaagtcat gtacttcaag gacttcaact ccggcgcgat
      661 gatccagaac gtcgtcgaca gggcgaagaa gaacgcgatc aagtcggtgt tggagaccgg
      721 tcagcccggt ctgcggatcc agcacctgct cgactcgatc gtcgacgagt tcgcggagaa
      781 cgaggacctg cccaacacca ccaatcccga tgactgggcg cggatttcgg gcaagaaggg
      841 cgagcggatc gtctacatcc gcacgctcgt caccggcaag tcgtcaagcg cgtcgcgggc
      901 gatcgacacc gagtcgaacc tgggtcagta cctctaggtc tcattccgcg gatgtgcgtt
      961 tctgcacgcc gacaccccgg ttatcgcgtg cattttgcgc acgctcacgg cccacggggt
     1021 tacgttggcg gacatgtccg acgatgtcac agtgatcgac gatgtgtacg ggaagcggta
     1081 cggcgagatg cttctcgtca cgaccacgcc tgcgggaccc gaggcgaccg tttacaagac
     1141 ctttccactc aacgactgtc ccgtcgagct gtgggacaaa ctggatccgc aagctatcgc
     1201 ggcagacaac ggcgccgttg ccgcactgct caacgggccg cgatattggc tgatgagcag
     1261 catcggcaag cgaagtcgag agaccttgga gcgcaagagt ttcggtggta tcgagatgat
     1321 ccggcaagcc accgtcaaac tcgcctcgat gaatccctcg ccctacagcc tgaatcgggt
     1381 ggaccgcaag gcggtgttca ccttcgacgc gggccgcgag atctacgagc tcgtcgaccc
     1441 cgatggtcgg cgttgggtca tgcagacgtg gagccagact gtcgatcccg cactgtcact
     1501 gagcgacctg ccgggcctcg caagccggct gtcgttaccg gacgactgga cgtaccggcc
     1561 gcgcacgccg acatcatcac tgcgcgttga caccacaagt caggacgcat gcgtgaggca
     1621 agacgacctg gccaacagct attcactgca gacgtcgggc acgcgaaagc ccccgaaatc
     1681 gcagggattc gggggctttg cggcacggct tgtcagcaga ggtggatgac cgccgcctcg
     1741 tagatgtact gcgcatctgc cacttcgatg gaatcgccgg tgttttcgta gatggccttg
     1801 accgtgttgt tttgggggac accgtgttgc aggtcggtgc agatctggta gcccatgtcc
     1861 agcgcaacat cggtcggccc ggtgaagtcg ttgttggcca ggtcggtgag atagctctcc
     1921 tcgtctgcga atgccggagc tgcgggggtt cgcagcaaat tagccgatct tgtaggctcg
     1981 gtagctccgc gttagcgggc accctccatt tccaagtagg cgtcgcggcc ggccagactg
     2041 accgccacat cgtcgatcag gggggtgaag aaccgctcgc ggtatagcgg gtcgacgctg
     2101 gtactcacgg tgaagtacaa accggacagc accgcgacga acaaggacac atgcacgatg
     2161 gtctggggaa ccggtacatg gatgccgaac caggtgcccg gacaaggtgg ttcgccaacg
     2221 gggcacgtct cctctgagga ccacagcacg gcgacgtcgt ccggtaccgc gacgatgccg
     2281 agcgccagga agaacgcgaa cagccctgcg gtgaagaaca ccacctggat ggcctgcgac
     2341 accaccatca ccgcaacgac attgacctgc tcggcaagcg acagcggcgt gcgcaccggc
     2401 tggtgaccgg ctgcggtttc cagcggtgtg ccttcgagca gcttggcggg atcgctttgt
     2461 tccgcgcggt cctcccgaag cgcgcgcact tcgtcacgaa tagtggtcac cacgaatatg
     2521 agggcgacga gcgcgaggaa tccgattgtc tgccaaagtc tttggcgagt caatcgggcg
     2581 gacagctgcc acagctcgcc ggtgaagtac accaccacgg tgatcatcag cagcggaagt
     2641 gcacggctca tcagtgtgcc caatgcgccg aactgcaccc aggcaaagcg gaacgcccac
     2701 aacgcaatcg agccgaaccc gagataggtc aaccagatcg ccagtagtgc gacgaccgcg
     2761 aatcccaccg cttcggccgc ggcgatccct gagaacccgc tgtcggcgaa gggaagtccg
     2821 aacacgaaga tcgccatcac cagcagcgcc gccgaccgtc ggcctgcttc accgagtgtg
     2881 gtgccagtgc ggtgcagcag atacagcgcg aacggggcgg cgatgagcag ccccgtgagt
     2941 atcccgaggc ggatcacata gccgaaatcg ggatcgccgt cagtgagttc ggccagcatc
     3001 atcgtcagcg atatcaccgc accgatgcca ctcaccatcg gtgccgagcg ttcgatcaag
     3061 gcgcgagagc gaacccgtcg ggtgagcacc agcggcagcc cgcgctgcaa gaaccattcg
     3121 tgcacctgcc gcatgtcgga cacgggcaat attgtgcgcg attgcggccg tagtggaggt
     3181 tcagcgcgcc gataatgccg cgcggtaccg attgcgcgcc gtcaacgtga gccgaaggtc
     3241 gtcgatgagc gggtcgaaga atcgtgtccg gtagtccccg tcgctcacgg tgcgcgcgct
     3301 gatgtacata aacgtcagcg cgccaaggaa aaacgtgatc tgaatcagcg aatccgggac
     3361 tgggatcgtc atgccgagca cggtcccgtc gctgctgccg ttacgtgtcc acgccgcaag
     3421 caattcgggc gacaagagga tgaggccgag gatcaggaag agcagagagg tcatcgccgc
     3481 gatgatcagg atctggctga gcagcgatgc ggcgaccaca aacatgacgt tgagctgctc
     3541 aacgcggctg agcgtacggg cgctcgccgg atccggcatc gtctcgaacg gtgtgcctgc
     3601 cagtcgcact gagtggtcgt gggccggctc atcggaatcc agcatcggct tcacccggtc
     3661 ctgcgacacc gacaccacaa acaccgccgc gatcgcggtg agaaacgcca gcgccagcca
     3721 caagcgcggc cgcgaaaccg tcgcggccat cagccacacg ggggagttga agaacaccaa
     3781 gatcgtcagc agcagcagcg gaagggcacg cgtcaccatc gatcccgccg ccgcaaggtt
     3841 cgacgttgtc atctgaactg cccagccaag gatcgagccg gcgccgcatg cggtcagcgc
     3901 caggatggcg gcgatcacga tcgcttcaaa gatgaggtcc acaagaatcc gagggctggg
     3961 cccgccgagg acgccgcctg cgaccgcgac cgtcgtcgcc accgtggccg cgacggtgcg
     4021 gccgggcacc gtcgggattc gggacacgag ccagcccacc aacaccgcga tcggcagcac
     4081 gaggatgagc agcgccagga cgaaccattc cgttcgtgtc ggatggccgt tgatgtcgat
     4141 ggtgtgcttg ccggtgacgg ccacgacgag cgcggagttg gccatgaaaa ccgcgaaccc
     4201 ggccagcacg ggcgccgacc gtggccacag ccgccgcacc agtgcgcccg gccgcagcac
     4261 cgcgggcaga ccacgatcca agaaccacgc atcggcagcg cgacgctgcg tcacgtcaat
     4321 catgaaaggc ccgccggttc ggtgctgacg tcgcctggat cgctgcccgc cacagcagca
     4381 gcccggtctg cacgccgacg gcgatcgaca tcatcgagat cagggtggcg gtgaagacgg
     4441 cggtgctgtg ggtgccgagc agctgggtga cgcccatgaa gccgagcgac ccggggacca
     4501 gtagccagaa gccgggcagc accagtgaca cggtcggcgg tgtgttcggg cggtgcgaga
     4561 tggccagtgc acagatgatc agtgtcaggc cgccgccaaa gccgcttgca tagcttccca
     4621 gcacggcgtt gccgacccat tgacctgcgt atgcggtcag cagcatcagc aacatccacg
     4681 gcagaaacga tgtcggcggg ccgaagtaca gcatggcgcc gatgccgtag acgaacacgc
     4741 caagcagcgg tgcccatgcg cccaaccggt tgatctcggt ggtgacgagg ttggtgtcgg
     4801 cgatgccggc cacctgggcg gcgatcagga tgccgaatgc caactgcgcg atctgcatga
     4861 aacccgcgac gagcctggct tgcgccggat acgacgtggt gcgtcgacag ttcgatgacc
     4921 gcgagcgtga tcgccgcgcc gggcaggaag gtggcaaggg gtgcggccag cgcacggagg
     4981 ctgtcgacgc cgacgtgcca atggttgttg aacgtgaaca cgatcagcgc gacgaggaac
     5041 gagcagatca gcggaagcag atatccgatg ccctcgacgc ggcgaccgag cgccgacagc
     5101 gcgccgacca ggagccccag aatcgctgcg ccaaccaggc tccatggcgt cggctgcaaa
     5161 atcagcgcga gcccggtgct ttgcactgca tagcccatga tcgtgaccca gaacggaaat
     5221 cgcctgcgct ggttacgaat tcggtcaagc tcggccatgc catcttcggg atcgatcgtg
     5281 cccgtcggtg cccgtgcgac cagttgtgct cgagggaacg actggtcgaa gcgcacatcg
     5341 aaatcgggat cggcgatgaa catgccctcg ccggtggggc tgccaacctg gacgtagttc
     5401 ggcagcgcca ggaattgatg gtcgagcccg tacgcgcgcg acgtctgttc catcaccgcg
     5461 cgcaccatgg ttaccgggta attcgcggcg gccattgcgc cgccgagcgc ggccacaaac
     5521 tggcccgcgg ccgcatttcg ttcatccact tcggatggca cggtcgcagg ctactgccca
     5581 gcgtccctta cgctgggcga catgcaacgg attatcggaa ccgaggtcga gtacggcatc
     5641 tcgtcgccgt ccgatccgac cgcgaatccg atcctgacgt cgacgcaggc tgtgcttgcg
     5701 tatgcggccg ccgcggggat ccagcgcgcc aagcgcaccc gttgggacta cgaggtggag
     5761 tcgccgctga gggacgcgcg gggattcgac ctgagccggt cggcgggccc gccgccggtg
     5821 gtggacgccg acgaggtcgg tgctgccaac a
//
LOCUS       DASRJA010000031         2942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2509328,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000031 DASRJA010000000
VERSION     DASRJA010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2509328"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFC01_01440"
     CDS             <1..669
                     /locus_tag="VFC01_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN78338.1"
                     /translation="DITDTDRIRAAVAEVLDGHGSIDVVVNNAGRGAVGAAEETSDGE
                     LRDLMDLHFFGPAALTREVLPHMRERGTGAIVQMSSMGGRFSFPGVGAYSATKFALEG
                     WSEALAKEVAPFGIRVLIVEPGAFRTSFNASGALLLSKRIPAYDEQIDAVRNTLADDD
                     GKQPGDPAKAAAAILQALDSDDPPLRLALGGDAVDAITQSLENAKAELAAWESVGRSA
                     VFDD"
     gene            854..1795
                     /gene="ltnD"
                     /locus_tag="VFC01_01445"
     CDS             854..1795
                     /gene="ltnD"
                     /locus_tag="VFC01_01445"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: protein motif:HMM:NF043037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HZN78339.1"
                     /translation="MNVLPADTSSPSPRVGLVGLGSMGLGMARSLRAAGYEVSVYDVR
                     REVAVSFAAEGGTAHQTVAELATETDVLISVVVNAAQTEAVLFGTGDDGAIDRLKPGS
                     VFVMCSTVDPQWSAALAGTLAERGIHYLDAPISGGAIKAAAGQLTMMTSGSPEAYALA
                     DPVLEAMSEAVYRLGDKPGMGSVVKIVNQLLAGVHIAAAAEAMALGIRCGLRADDIYE
                     VITHSAGNSWMFENRMAHVLTGDYTPHSAVDIFVKDLGLVLDTARPNVFPLPLAAAAH
                     QQFLRASAAGHGREDDSAVIKTFPGVDLPTSNGTEEL"
     gene            1798..>2942
                     /gene="otnK"
                     /locus_tag="VFC01_01450"
     CDS             1798..>2942
                     /gene="otnK"
                     /locus_tag="VFC01_01450"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: protein motif:HMM:NF043035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HZN78340.1"
                     /translation="MPVRLGCIADDFTGATDLANNLVRAGMRVVQTINTPAADTAIDA
                     DAVVIALKSRTIPAADAVAQSRQALAWLRLAGAQQIYFKYCSTFDSTPKGNIGPVIDA
                     LMDDLGTEFTIATPAFPDNGRSVFKGHLFVGDQLLSESGMKDHPLTPMTDSDLVRVLT
                     PQTRHRVGLIDYATVAAGRDAVRARIDQLHADGVGIAIVDAITNKDLLELGPAIADLR
                     LVTAGSGVAIALPANWGFRPSPDAAALPAATGLQAIVSGSVSVATNTQVAEFHRTGRP
                     SFQVDALRIAAGQDVVTEALGFADRHLASGPVLFYSTQAPSGVRAVQNALGAAKAGSL
                     IEDTLARIAAGLVERGVGQLVVAGGETSGAVVQELGIDAMRIGPQIDP"
BASE COUNT          491 a          997 c          949 g          505 t
ORIGIN      
        1 gacatcaccg ataccgaccg cattcgcgcc gccgtcgccg aagtcctcga cgggcacggg
       61 agcatcgacg tggtggtcaa caacgccggc cgcggagccg tcggtgccgc cgaggaaacc
      121 agcgacggtg agctgcgcga cttgatggat ctgcacttct tcggccctgc cgcgctgacc
      181 cgcgaggtgt tgccgcacat gcgggagcgg gggaccggcg ccatcgtgca gatgagctcg
      241 atgggcggcc ggttctcatt cccgggcgtc ggcgcttatt cggcgacgaa gttcgcgctg
      301 gaaggctggt cggaggcgct ggcaaaggaa gtcgcgccct tcggcatcag ggtgctgatc
      361 gtcgaacccg gcgcgtttcg gacgtcattc aacgccagcg gcgcgctgct gctgtccaag
      421 cgcattccgg cctacgacga acagatcgac gccgtccgca acacactcgc cgacgacgac
      481 ggcaagcagc ccggcgatcc ggccaaggcc gccgcggcga tcctgcaagc actcgattct
      541 gatgacccac cgctgcggct ggcgctcggc ggtgacgccg tcgacgcgat cacccagtcg
      601 ctcgagaacg cgaaagccga actggcggca tgggaaagcg tcggccggtc cgcggtgttc
      661 gacgactagg tcggcgtctc ctccatctgg ccgcggacgc cgtggtggcg gtgttcctcg
      721 atggcgcact aacgcgctag ctggcgggtt tagccctcga agcggccctg gccgtctgtg
      781 aaccagacca tatggcccaa aaaatgtggc gcagggcact tgatttctag tcatcaggtc
      841 atatgatgac ctgatgaatg tgttgcctgc agatacctct tcgccttcgc cgcgcgtcgg
      901 cctcgtcggt ctgggctcga tgggcctggg catggcgcgt tccctgcggg ccgctgggta
      961 cgaagtgagt gtctacgacg tccggcgcga ggtggctgtt tcgttcgccg cagagggcgg
     1021 aacagctcac cagacggtgg ccgagctcgc cactgagacc gacgtgctga tcagcgtcgt
     1081 cgtcaacgct gcacagaccg aggcagtgct gttcgggacc ggcgacgacg gagcaatcga
     1141 ccgtctgaag cctgggtctg tattcgtgat gtgctccacg gtcgatccgc agtggtcggc
     1201 cgcgcttgcc gggacgctcg ccgagcgcgg catccactac ctcgacgcgc ccatctccgg
     1261 tggtgcgatc aaggccgctg caggccagct gaccatgatg acatccggct cgcccgaggc
     1321 atatgcgctc gccgacccag ttctcgaggc aatgagcgaa gccgtgtacc ggctcggcga
     1381 caagcccggt atgggctcgg tggtcaagat cgtcaaccag ctgctggccg gggtgcacat
     1441 cgccgcggcc gccgaggcga tggcactcgg catccgatgc ggactccgcg cggatgacat
     1501 ctacgaagtc atcacgcaca gcgccggcaa ctcgtggatg ttcgagaacc ggatggcgca
     1561 cgtgctcacc ggcgactaca ccccgcactc cgccgtcgac atctttgtga aggacctcgg
     1621 cttggtgctt gataccgcgc ggcccaacgt gtttccgctt ccgctcgcgg cggcggcaca
     1681 ccagcagttt ctgcgcgcgt cggccgccgg tcacggtcgc gaggacgaca gcgccgtcat
     1741 caagacgttc cccggagtcg acctgcctac cagcaacggc accgaggagc tgtgaaaatg
     1801 cctgtccgcc ttgggtgtat cgccgatgac ttcaccggcg cgaccgatct ggccaacaat
     1861 ctggtccgcg caggcatgcg cgtcgttcag accatcaaca cccccgccgc ggacacggcc
     1921 atcgatgccg acgccgtggt gattgccctc aagtcgcgca caatccctgc cgccgacgct
     1981 gtggcacagt cccggcaagc cttggcatgg ttgcgtttgg cgggcgcgca gcagatctac
     2041 ttcaagtact gctctacctt cgactccacc ccgaagggca atatcggtcc cgtcatcgac
     2101 gcgctgatgg acgacctcgg caccgagttc actattgcca caccggcatt ccccgacaac
     2161 ggccggtcgg tgttcaaggg ccacttgttc gtcggcgatc aactgctcag tgagtcggga
     2221 atgaaggacc atccgctcac cccgatgacc gactccgatc tggttcgcgt gctaactccc
     2281 caaacccggc accgggtggg gctgatcgac tacgccaccg tcgctgcggg ccgcgatgct
     2341 gtgcgcgcgc ggatcgacca gctgcacgca gacggcgtgg ggatcgcaat cgtggacgcg
     2401 atcaccaaca aagacctgct tgagctcggt ccagccatcg ccgacctacg tctcgtcacc
     2461 gccggatccg gcgtcgcgat tgccctgcct gccaattggg gattccgtcc ctcgccagat
     2521 gcggccgcac tccccgccgc tacgggtctg caggcgatcg tctccggctc ggtatcggta
     2581 gcgaccaata cccaagtcgc cgagttccat cgcaccggac ggccgtcatt tcaggtcgac
     2641 gcgctgcgca tcgccgccgg ccaggatgtc gtcacagagg cactcggatt cgccgatcgg
     2701 cacctcgcgt cgggacctgt gctgttctac tccacccagg cgccttcggg ggtgcgtgcc
     2761 gtgcagaatg cactcggcgc ggccaaggcg ggctcattga tcgaggacac cctggcacgg
     2821 atcgcggccg gcctcgtgga acgcggcgtg ggccagctgg tcgtcgccgg cggcgagaca
     2881 tccggggcgg tcgtccagga gctgggcatc gatgccatgc gaatcggtcc ccagatcgac
     2941 cc
//
LOCUS       DASRJA010000032         4466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1105231,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000032 DASRJA010000000
VERSION     DASRJA010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105231"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /gene="pruA"
                     /locus_tag="VFC01_01455"
     CDS             <1..1407
                     /gene="pruA"
                     /locus_tag="VFC01_01455"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HZN78341.1"
                     /translation="AQHADASAAIDAAIAAKADWEATPFDERAAVFLRAADLLAGPWR
                     EKIAAATMLGQSKTAYQAEIDSPCELVDFWRFNVAFARQILAQQPISSRGVWNRTDYR
                     PLEGFVYAITPFNFTAIAGNLPTAPALMGNTVVWKPSPTQTFAAYLTMQLLEAAGLPP
                     GVINLLAGDGIAVSDVALADPRLAGIHFTGSTGTFQHLWREVGTNIDRYHTYPRLVGE
                     TGGKDFVLAHSSARPDVLRTALIRGAFDYQGQKCSAASRAFVPRSVWQQMGDDFLAAT
                     EALRYGDVTDLTNYGGALIDERSFAKNVKAIERAKSAAGVTIAAGGEYDDSEGYFVRP
                     TVLLSDDPGDESFCTEYFGPILSVHVYPDSEYDQILDIVDKGAKYALTGAVIADDRAA
                     VLAAEQRLRYTAGNFYVNDKPTGAVVGQQPFGGSRASGTNDKAGSALNLLRWTSARSI
                     KETFVPPTDHNYPHMETE"
     gene            1407..2372
                     /locus_tag="VFC01_01460"
     CDS             1407..2372
                     /locus_tag="VFC01_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HZN78342.1"
                     /translation="MAELFHRVARPAILGASRWDGLRRTAERLPVTREVVHRFVPGET
                     VADAMNAVEQLRYSGRLISIDYLGEDVTDADTANATVDAYLGLLDAMGSRSEPASDVR
                     PLEVSLKLSALGQALPRDGEKIALENAHAICERAQKVGAWVTVDAEDHTTTDSTLSIV
                     RDLRTEFDWLGTVLQAYLKRTEADCREFAASGARIRLCKGAYDEPASVAYRAGDEVTS
                     SYLRCLEILMEGSGYPMVASHDPEIIAAVPALARETGRGVDDFEYQMLYGIRDAEQRR
                     LADEGNHVRVYVPFGTQWYGYFVRRLAERPANLTFFLRALAERRH"
     gene            complement(2369..2764)
                     /locus_tag="VFC01_01465"
     CDS             complement(2369..2764)
                     /locus_tag="VFC01_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HZN78343.1"
                     /translation="MSDEDAIRELIVRWADAVHTGDLETVLAERDPDIVMFDVPPPYD
                     GLRGIGAYRDSWPPFFEWQRQGAIFEIVELDVTAGDDVAFAYALARCGKPEDFAKNPD
                     SRLRMTFGLRKRDGRWVVTHEHGSFPMIG"
     gene            complement(2947..3480)
                     /locus_tag="VFC01_01470"
     CDS             complement(2947..3480)
                     /locus_tag="VFC01_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZN78344.1"
                     /translation="MNTKAKNLDGYGTPPIEWERVRDVLTSDITQGPDAGGPNRHTTW
                     LTTINPDGSPHVTPVGAIQLDGVWYFTSGPGTRKSRNLAADPRCVVSVATHPFDLVLE
                     GSAARVTDADELRSVADEYNRGGWPAQPEGDALTAEYSAPSAGPAPWHVYKVAPTTVF
                     AFGTAEPYGATRFDVTR"
     gene            complement(3511..4389)
                     /locus_tag="VFC01_01475"
     CDS             complement(3511..4389)
                     /locus_tag="VFC01_01475"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN78345.1"
                     /translation="MELRELRYVLAIDKTGNLSRAAEVLHVSQPALSYALRKLERELG
                     VQIFDRHSGGVKPTVAGKDVIAAAKRTVREADRIAELAQAHADGQRGVLRIGFEATGA
                     GELTTRARSEFTRTHPAVRVQPKRYDWGGEADALRDGAVDAAFLWLPADTSGMHVEVV
                     HTEPRIAAVWRGHRLARRKSISILDIRDDPLMWTDKAPRAWVDWWAVNPRPDGSAPVW
                     GPTNDNVEEMLEQVAEGRAVCIVPESMGSFYGRPDLCWIPIADIEPLRVALAWMKPGS
                     KLVSEFAAVVRELASR"
BASE COUNT          753 a         1468 c         1490 g          755 t
ORIGIN      
        1 gcccagcacg ccgatgcgtc cgccgcgatc gacgcggcaa tcgcggccaa agccgactgg
       61 gaggccaccc cgttcgacga gcgcgccgcg gtgttcctgc gcgccgccga cctgctggcc
      121 ggaccgtggc gggagaagat cgcggccgcc accatgctcg ggcagtccaa gaccgcctat
      181 caggccgaga tcgactcgcc gtgtgagctc gtcgacttct ggcggttcaa cgtcgcgttc
      241 gcgcgacaga tcctggcgca gcagccgatc agcagccgcg gtgtgtggaa ccgcaccgac
      301 taccggccgc tggaaggctt cgtctacgcg atcacgccgt tcaacttcac ggcgatcgcc
      361 ggcaatctgc cgaccgcgcc cgcgctgatg ggcaacacgg tggtctggaa gccgtctccg
      421 acgcagacct tcgccgcgta cctgaccatg cagctgctgg aagccgcggg tctgccgccg
      481 ggtgtgatca acctgctcgc cggcgacggc atcgcggttt ccgatgtggc ccttgcagat
      541 ccgcggctgg ccggcattca cttcaccggg tcgacgggta ccttccagca cctctggcgc
      601 gaggttggca ccaacatcga ccgctatcac acatacccgc ggctcgtcgg ggagaccggc
      661 ggcaaggact tcgtacttgc gcactcgtcc gcgcggccgg atgtgctgcg cacggcgctg
      721 attcgtggtg cattcgacta ccagggccag aagtgctcgg cggcgtcgcg cgcgttcgtc
      781 ccgcgatcgg tctggcaaca gatgggtgac gacttcctcg cagccaccga agcgttgcgc
      841 tacggcgacg tcacggacct gaccaattac ggtggcgcgc tgatcgacga gcggtcgttc
      901 gcgaagaacg tcaaggcgat cgagcgtgcc aagagcgccg ccggggtgac catcgccgcc
      961 ggaggcgaat atgacgacag cgaaggctat ttcgtccggc cgacggtgct gctgtccgac
     1021 gatccgggcg acgagtcgtt ctgcaccgag tacttcgggc cgatcctgtc agtgcacgtc
     1081 tatcccgatt cggagtacga ccagatcctc gacatcgtcg acaagggcgc gaagtacgcg
     1141 ttgaccggtg cggtgatcgc cgatgaccgc gcggcggtgc tggccgccga gcagcggctg
     1201 cggtatacgg caggcaactt ctacgtcaac gacaagccca ccggagcggt ggtgggccag
     1261 cagccgttcg gcgggtcgcg ggcgtcgggc accaatgaca aggccggctc ggcgctgaac
     1321 ctgctgcgct ggacgtcagc ccggtcgatc aaggagacct tcgttccgcc gaccgaccac
     1381 aactacccgc acatggagac cgaatgatgg ccgagctgtt ccaccgggtc gcgcgcccgg
     1441 cgattcttgg cgccagccgg tgggacggat tacgacggac cgccgaaaga ctgcccgtca
     1501 cccgtgaggt ggtgcaccgt ttcgtcccgg gcgaaaccgt cgcagacgcg atgaacgccg
     1561 ttgagcaact acggtattcg ggtcgtttga tcagcatcga ctacctcggc gaggacgtca
     1621 ccgacgccga caccgccaac gcgaccgtcg acgcctacct gggcctgctc gacgcgatgg
     1681 gcagccgcag cgagccagcg tcggacgtgc ggccgctgga ggtgtcgctg aagctgtcgg
     1741 cgttggggca ggcgctgccg cgcgacggcg agaagatcgc gctggagaac gcccatgcga
     1801 tctgcgaacg ggcgcagaag gtgggggcgt gggtgacggt cgacgccgaa gaccacacca
     1861 cgacggactc gaccctgtcg atcgtgcggg atctgcgcac cgaattcgat tggctgggaa
     1921 cggtattgca ggcctacctg aagcgcaccg aggccgactg ccgagagttt gcggcgtcgg
     1981 gcgcccggat ccggttgtgc aagggcgcct acgacgagcc agcgtcggtg gcctaccgcg
     2041 ccggcgacga agtcacatcg tcgtacttgc gctgcttgga gatcttgatg gaggggtccg
     2101 gctatccaat ggtggcgtcg cacgatccgg agatcatcgc ggcggtgcct gcgctagccc
     2161 gtgagaccgg ccggggcgtc gacgatttcg aataccagat gctgtatggc attcgcgacg
     2221 ctgagcaacg gcggctggcc gacgagggca accacgtccg cgtctatgtg ccgttcggca
     2281 cgcagtggta cggctacttc gtgcgtcggc tggccgagcg gcccgcgaac ctgacgttct
     2341 tcctgcgggc gttggccgaa cggcggcact aaccgatcat cgggaacgat ccgtgttcgt
     2401 gggtcacgac ccagcggccg tcgcgcttgc gcaggccgaa ggtcattcgc aggcgactgt
     2461 cgggattctt cgcaaagtcc tccggtttac cgcagcgcgc tagcgcatac gcgaatgcca
     2521 cgtcgtcgcc ggccgtcaca tcgagctcga cgatttcgaa gatggcaccc tgccgctgcc
     2581 actcgaagaa cggtggccag gagtcccggt aggcgccgat accgcgaagg ccgtcgtagg
     2641 gcggcggcac gtcgaacatc acgatgtccg gatcacgttc ggcgagaacg gtttcgagat
     2701 cgcccgtgtg aacggcgtcg gcccagcgaa caatcagttc gcggatggcg tcctcatcgc
     2761 tcatcggctt tacatccctt gcgctcggtg gtgcccgcgg atgacacgag gacatagccg
     2821 gcgctggcaa acagcacggg catcattgcc atcatcccga atactgcggt catcatgtca
     2881 cccctttcgg aagctctttc gctctttagt atgttcaacg gtatagacaa gtgcgcggtt
     2941 caaaactcat cgcgtcacgt cgaatctggt ggcgccgtag ggctcagcgg tgccgaacgc
     3001 gaacacggtg gtcggcgcga ccttgtagac gtgccagggt gccggacctg ccgagggcgc
     3061 actgtattcg gcggtcagag cgtcgccctc gggttgcgcg ggccaacccc cgcggttgta
     3121 ttcgtcagcg acggaacgca gttcgtcggc gtcggtgacg cgggcggcgc tgccctcaag
     3181 caccaggtca aacgggtgcg tagcgacgct gacgacgcag cgtggatccg ccgcgaggtt
     3241 gcgggacttg cgcgtgcccg gtccggaggt gaagtaccag acaccgtcga gttggatggc
     3301 gcccaccggc gtcacgtgcg gtgagccgtc ggggttgatg gtcgtcagcc acgtcgtatg
     3361 ccggttcggt ccgccggcat caggtccttg ggtgatgtcg gacgtgagga cgtcgcggac
     3421 ccgctcccac tcgataggag gcgtgccgta gccgtcgagg ttctttgctt tggtgttcat
     3481 gcccgtatag acaaggcccc acgcgagaac tcatcgcgac gccaactctc gcactaccgc
     3541 ggcgaattcg gacaccagct tcgagcctgg cttcatccac gccagcgcca cccgcagcgg
     3601 ctcgatgtcg gcgatcggaa tccagcacag gtccggacgg ccatagaagc tgcccatgct
     3661 ctcgggcacg atgcacacgg ctcggccctc ggccacctgc tcgagcatct cctcgacgtt
     3721 gtcgttcgtc ggcccccaca ctggcgccga cccgtccggc cgaggattca ccgcccacca
     3781 atccacccat gcgcggggcg ccttgtcggt ccacatcagc ggatcgtcgc ggatgtcgag
     3841 aatcgatatc gacttgcgtc gggcaagccg gtggcctcgc cacaccgcgg cgatacgcgg
     3901 ctcggtatgc accacttcga cgtgcatgcc tgacgtgtcg gcaggcagcc acaagaacgc
     3961 ggcgtcgacg gcgccgtcgc gcagcgcatc ggcctcgccg ccccagtcgt agcgtttggg
     4021 ctgcacccgc accgccgggt gggtacgggt gaactccgag cgggcgcgag tcgttaactc
     4081 gccggcgcca gtggcctcga agccgatccg cagtacacca cgctgcccgt cggcgtgcgc
     4141 ctgggccagc tctgcaatcc gatctgcttc gcgcacagtg cgtttcgccg ccgcgatcac
     4201 gtcctttccg gcgaccgtcg gcttgacgcc gccagagtgg cgatcgaaga tctgcacgcc
     4261 gagctcgcgt tccagcttgc gcagcgcata tgacagcgcg ggctggctga catgcagcac
     4321 ctcggcggcg cggctgaggt tgccggtctt gtcgatggcg agcacgtagc gcagctcgcg
     4381 cagctccatg gccgaactat aaacctcgtt tatggcaggt accggaatct gtattggaca
     4441 acccggccgc ggtaggggat agataa
//
LOCUS       DASRJA010000033        23494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_808334,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000033 DASRJA010000000
VERSION     DASRJA010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 23494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23494
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808334"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFC01_01480"
     CDS             complement(<1..556)
                     /locus_tag="VFC01_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN78346.1"
                     /translation="MPVEWVTAPHEPALELIETGIRDQAGTVLIGAAGVGKTWLARAA
                     ADQLASQFGRVDWVTATAPAVPFAPFNHLIEVSDTEKTAAVLRAARESLSDGRLLIVD
                     DAHLLDKLSAALVYQLAVSGAVKLIVTLAPNEPVPDGISALWRDDLLARIDVAPAGHD
                     DSRQATMVEEFVATLPGPARRVLEY"
     gene            585..1145
                     /locus_tag="VFC01_01485"
     CDS             585..1145
                     /locus_tag="VFC01_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZN78347.1"
                     /translation="MSPEPEREWAVCVYCASGPTHAELLALAVQVGEAIAERGWTLVS
                     GGGNVSAMGALAGAARARGGYTVGVIPKALVHREVADTEADELVVVDTMRERKQVMED
                     RADAFIALPGGIGTLEEFFEAWTAGYLGMHDKPVVMLDPIGHYDGLLAWLHGLVDSGY
                     VAQGALDRLVVVDNVEVAIEVCAPAS"
     gene            1280..3064
                     /gene="fadD6"
                     /locus_tag="VFC01_01490"
     CDS             1280..3064
                     /gene="fadD6"
                     /locus_tag="VFC01_01490"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase FadD6"
                     /protein_id="HZN78348.1"
                     /translation="MTDEKSGTRSSVGLLDIASQVPGLLMDLPTILSGVITGFGARPS
                     AKTSIGKVFQERAARYADKVFIKFGDDQLTYREANEAVNRYAAVLAARGVGHGDVVGI
                     MLRNSPQSVLLMLATVKCGAIAGMLNYHQRGDVLKHSLGLLSAKAVVAETEFIEPITD
                     SGADTSGVMTVDELQRLSATAPTGNPATTAAVLAKDKAFYIFTSGTTGMPKASVMTHY
                     RWLRALAGFGGLGMRLSSNDTLYCCLPLYHNNALTVALSSVLNSGATLALGKSFSASK
                     FWDEVIRYDATAFIYIGEICAYLLNQPPKDTDRKHKVRVIGGNGLRPAIWDEFTERFG
                     IERVCEFYAASEGNTAFVNFLNLDKTTGICPTPVAFVEYDPDSGEPVRDENGRVRKVR
                     NGEPGLLLSKVSSFQPFDGYTDKKASEKKLVRNAFRDGDVWFNTGDLMRSQGFGHAAF
                     TDRLGDTFRWKGENVATTEVEAAVSTDPQVEEATVFGVEVEGTGGRAGMAAIQLKEGE
                     EFDGKALAKAAYERLPGYAVPLFVRVVQELAHTSTFKSQKVDLRKEGYDTEEVEDPIY
                     VLAGRDEGYVEFYDEYPKEVADGKKPKN"
     gene            3200..4024
                     /gene="folP"
                     /locus_tag="VFC01_01495"
     CDS             3200..4024
                     /gene="folP"
                     /locus_tag="VFC01_01495"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZN78349.1"
                     /translation="MAIVNRTPDSFYDRGATFTDEAAKTAAHQVIEEGADVVDVGGVK
                     AGPGDTVGVDEEIARVVPFIEWLRDTFPNQLISVDTWRSEVAKQACAAGADLINDTWG
                     GHDPALAEVAAEFKAGLVCSHTGGATPRTRPFRVNYGISERGVVDDVIAEVTAAAERA
                     VAVGVARDAVLIDPTHDFGKNTYHGLSLLRHVKDLVKTGWPVLMALSNKDFVGETLGV
                     GLTERLEGTLAATALAAAEGAAMFRVHEVGPTRRVLEMVASIQGVRPPTRTVRGLA"
     gene            4095..5045
                     /locus_tag="VFC01_01500"
     CDS             4095..5045
                     /locus_tag="VFC01_01500"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HZN78350.1"
                     /translation="MTLTPELPGTDVVTGRWLTDHSWNRPTWTVDELIAAKAGRTISV
                     VLPALNEEETVGSVVETITPLLGGLVDELIVLDSGSTDDTEIRALAAGARVISREVAL
                     PEVAPQPGKGEVLWRSLAATAGDIIVFVDSDLIDPDPMFVPKLVGPLLTTDGVHLVKG
                     FYRRPLKVSGSEDANGGGRVTELVARPLLASLRPELSCILQPLGGEYAGTRELLTSVP
                     FAPAYGVEIGLLIDTYDRLGLDAIAQVNLGVRTHRNRPLTELASMSRQVIATLLSRCG
                     VPDSGAALTQFFADGDDYTPRTSSVSLEDRPPMITLRPRG"
     gene            5111..6619
                     /locus_tag="VFC01_01505"
     CDS             5111..6619
                     /locus_tag="VFC01_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN78351.1"
                     /translation="MTVDVEYARRGDISIAYQTIGDGPVDIIFGAGLVSHLDLLWADP
                     YATAFFRRLASIGRLLLFDKPGTGLSDPVVGIPTVQQRVDDFLAVLDAAGSRRAVVIG
                     NSEAATPAILLAATHPERVEALIAMCGVPRITPSDDFLPGSEHRWERMWAIIRHSTDH
                     WGDGSLLQSLSPYARESAVYRRFAPNVERACASPGMARVMLNALWDYDVTGATHAVDV
                     PTLVVHRTGDILPIEAARWTADAIAGAKLLELPGPEPVCFFNGDDLLAGVEDFVGGSA
                     PRGPISRKLVTVLYTDIVESTARAVELGDERWSALLDAHHAAVRDQVERHDGTLIKTM
                     GDGMLATFDRPTLAVRCALAISRHAGEEGVQVRAGVHAGECEVSDHDISGIAAHIGSR
                     IMALAGPAEVLVSATVRDLVFGSGVEFEARGEHELKGVPGRWSVHHVVADRREDQRPA
                     FQATPELAALTPSPMTTMKPADRAIVGVANRAPRLSRFTLRMLSRRPVSTRG"
     gene            6659..7030
                     /locus_tag="VFC01_01510"
     CDS             6659..7030
                     /locus_tag="VFC01_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HZN78352.1"
                     /translation="MTLVLLYLVVLILVAVVLFGLGSVLFGRGESLPPLPRATTATVL
                     PASGVTGADVDAVKFTQTVRGYKTSEVDWVLDRLGQELDLLRGELAAVRAAHGIDDDY
                     EDADEPVEGGAHALPNGEDDS"
     gene            7063..7617
                     /locus_tag="VFC01_01515"
     CDS             7063..7617
                     /locus_tag="VFC01_01515"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HZN78353.1"
                     /translation="MDQSRLAPADFVLYRDYHDTEWGQPLRDSAALFERISLEAFQSG
                     LSWLIILRKRENFRRAFHGFDVDRIARYTKRDIDRLMADTGIVRNRAKVEATIANARA
                     AADLDVDLSELLWSFAPPPRPRPADLSQVPAVTPESTAMAKELKRRGFRFVGPTTAYA
                     LMQATGMVDDHTADCWVPAIKAAS"
     gene            7721..7888
                     /locus_tag="VFC01_01520"
     CDS             7721..7888
                     /locus_tag="VFC01_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003945169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HZN78354.1"
                     /translation="MAAMKPRTGDGPLEATKEGRGIVMRVPLEGGGRLVVELTPDEAA
                     ALGDELKGVTS"
     gene            complement(7938..9101)
                     /gene="glgA"
                     /locus_tag="VFC01_01525"
     CDS             complement(7938..9101)
                     /gene="glgA"
                     /locus_tag="VFC01_01525"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HZN78355.1"
                     /translation="MRVAMMTREYPPEVYGGAGVHVTELVAQLRTLCEVDVHCMGAPR
                     PGVIVAQPDPALKGANAALSTLSADLNMVNGASNATVVHSHTWYTGLAGHLAALLYGI
                     PHVLTAHSLEPMRPWKAEQLGGGYRVSSWVEKTAVEAADAVIAVSSGMRDDVLHTYPA
                     LDPNRVHVVRNGIDTDVWCPVQPQPGDSVLEELGVDLNRPIVAFVGRITRQKGVAHLV
                     AAAHYFAPEIQLVLCAGAPDTPEIAAEVTSAVQELARVRSGVFWVREMLPIGKIREIL
                     SAATVFVCPSVYEPLGIVNLEAMACATAVVASDVGGIPEVVVDRQTGLLVHYDASDPA
                     VFERRLGEAVNSLVADPERARQYGEAGRQRCIQEFSWAHIAEQTLEIYRKVSS"
     gene            9249..10463
                     /gene="glgC"
                     /locus_tag="VFC01_01530"
     CDS             9249..10463
                     /gene="glgC"
                     /locus_tag="VFC01_01530"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZN78356.1"
                     /translation="MRELPHVLGIVLAGGEGKRLYPLTADRAKPAVPFGGAYRLIDFV
                     LSNLVNARYLRICVLTQYKSHSLDRHISQNWRLSGLAGEYITPVPAQQRLGPRWYTGS
                     ADAIYQSLNLIYDEDPDYIVVFGADHVYRMDPEQMVQAHIESGAGGTVAGIRVPRAEA
                     TAFGCIDSDESGRIRGFIEKPADPPGTPDDPEQTFVSMGNYIFTTKVLIDAIRADADD
                     DHSDHDMGGDIIPRLVTDGMAAVYDFNNNEVPGATERDHGYWRDVGTLDAFYDAHMDL
                     VSVHPVFNLYNKRWPIRGEAEMLAPAKFVNGGSAQESVVGAGSIISAASVRNSVLSSN
                     VVVDDGAIVEGSVIMPGTRIGRGAVVRHAILDKNVVVGPGEMVGVDLEKDRERFAISA
                     GGVVAVGKGVWI"
     gene            complement(10494..11111)
                     /locus_tag="VFC01_01535"
     CDS             complement(10494..11111)
                     /locus_tag="VFC01_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HZN78357.1"
                     /translation="MELLTGFGLATAAGLNAYIPLLALGLLSRFTDLVTLPHGWAWLE
                     NGWVMTIVAVLLVVEIVADKIPALDSVNDLIQTFVRPTAGGIVFGSGTAAQTSAVTDP
                     GAFAQSGQWIPVAIGVVTALVVSLTKSTVRPAANVATAGVAAPVLSTIEDVFSVGLVF
                     LAVLVPVLVLVAVVVLIWAVVRIVRRRRRKAANLGPQRSLGPHRR"
     gene            complement(11119..11973)
                     /locus_tag="VFC01_01540"
     CDS             complement(11119..11973)
                     /locus_tag="VFC01_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN78358.1"
                     /translation="MPAESFEEHRSHLRAVAYRMLGSLTEADDAVQEAWLRMQRTDIS
                     DVRNVRGWLTTVVARICLDMLRTRSARREEPLDGAPAVAAPDAVDPEAEAVLADSIGV
                     ALLVILQTLNPAERLAFVLHDMFDLPYAEIAPIVGRTENSAAQLAARARRRVRGKTPD
                     PATDLVHQRRLVDAFLTAARDGDFDALLAVLDSDVVLHVDAAAAGTPTTIRGAQAVAA
                     NARAFSANARFAEPAVVDGAVGIVVAPKGKLALVLRFAIVGDKISEITIDAEPTRLRG
                     FSLALLDR"
     gene            complement(12028..12702)
                     /locus_tag="VFC01_01545"
     CDS             complement(12028..12702)
                     /locus_tag="VFC01_01545"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016892842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZN78359.1"
                     /translation="MKLLLQAVVASLFGLVFFAVPLFWPAGTFNYWQAWVFIAVFGVT
                     SFGPSVYWALRRPDILRRRMRSGPTAETRPVQKIATVGIAAMVLAVLVLSALDHRFGW
                     SQVPTPIVIVGDVLVAIGLGMSMLVVQQNNYAAATITVEAEQPVVDTGLYGLVRHPMY
                     AGALIMMVGMPLALDSYWGLVTIVPAVAVLLLRIDDEEKMLRQELAGYDEYTHKVHYR
                     LVPGFW"
     gene            complement(12713..13387)
                     /locus_tag="VFC01_01550"
     CDS             complement(12713..13387)
                     /locus_tag="VFC01_01550"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZN78360.1"
                     /translation="MKIALQAITSGLIGLVVFGLLVFWPAGTFDYWQGWTFIVVFAAS
                     TTIPSLYLAVTNPAALKRRMQAGPAAETRVLQKVIISIAFLAMGATIVVSALDFRFGW
                     SSVPATISVVGDVLVAVGLGIAMLTTIQNGYASANITVESGQQVMSTGLYSVVRHPMY
                     LGNVVLMVGIPLALGSYWGLVFVIPGVAILALRILDEEKVLTQELAGYPDYTNKVHYR
                     LMPGVW"
     gene            complement(13397..14071)
                     /locus_tag="VFC01_01555"
     CDS             complement(13397..14071)
                     /locus_tag="VFC01_01555"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016892842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZN78361.1"
                     /translation="MKIGLQIATTSLLGLALIGLALFWPAGTFDYWQAWVFLGIFVVL
                     SVIYTVYVGVKNPEVLRRRMNAGPTHESRPVQKVVSSGVVLTFFALLVVSGFDHRFGW
                     SNVGTTVALVGNVLAAVGLLITMVVVVQNSYAAANITVEADQTVVSTGLYGLVRHPMY
                     FGGLVMLIGMPFALGSYWAVVIVLVDLVLLAARILDEEKALTEELTGYREYTEKVHSR
                     LVPHVW"
     gene            complement(14083..15705)
                     /locus_tag="VFC01_01560"
     CDS             complement(14083..15705)
                     /locus_tag="VFC01_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HZN78362.1"
                     /translation="MTTATVAPRAGRHEAAQPGSPFTGVPSLLRLALRRDRIRLSVWI
                     AILTLMMVYAPNAVKLAYPEEPQRLARVNLLKTPAGMMLGGPMFGGNETDLGAMMANE
                     LMLTLIVAASILSISTVIRHTRAEEESGIAELVSSSVVGRYARTYAALILVGGVNAVL
                     AVTMTLAMASTGFAIVDTAGMCLGVTGVAMVFGAVAAVTAQLWRQARTATGAAMAALA
                     AAALVRGIGDVINNSGSALSWFSPIAWAQQMRAFVDLRWWPFALLVALAVALMALAAW
                     LESRRQYDDGNIPSTGEKPNARPITGVFGLHLTLQRGQTIGWTVGLFLGGLAFGSMTK
                     SLLDAAKDNPLLARVLTAQGTDGVYTTMTQFLAAAATAYVVTAVLRLYSDEQSGLGEA
                     VLAGAVSRWRWLLTAVASALVGSIVLMFFAGLGNGLGAGLTLGEPGTIVKLTLAGLAY
                     VPAMAVLAGVAAFAVAVKHAWIGWLAVTFVVVSLYLGALLRLPRWLLDLSPVGRTTVP
                     TDFPAVALAVMVVAAAALTILAGWIYRSRDAL"
     gene            complement(15702..16622)
                     /locus_tag="VFC01_01565"
     CDS             complement(15702..16622)
                     /locus_tag="VFC01_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN78363.1"
                     /translation="MTATSPRRDSSPAVEIRDLHKSFGRTKALDGLDMTVAHGEIAGF
                     LGPNGAGKSTTIRVLLGLLRADRGTVRLLGGDPWRDAVALHRRIAYVPGDVTLWPNLT
                     GLQVIDFLARLRGNGVDTRRRDQLIERFELDPHKKARTYSKGNRQKVAIVAAFSTNAE
                     LYILDEPTSGLDPLMEQAFQQCVNEVADRGAAVLLSSHILAEVEKLCDAVTIIRSGRA
                     VKSGTLDELRHLMRTTVTVRTRGDGQALQQVPYVHDFTSHNGQVRFSVDRNDLDFTME
                     HLTDLGIEELIVTPASLEDMFLREYQGASR"
     gene            complement(16594..17283)
                     /locus_tag="VFC01_01570"
     CDS             complement(16594..17283)
                     /locus_tag="VFC01_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN78364.1"
                     /translation="MRSASDDRTAVARIRDAAIEQWGQHGFNVGLRSIAEAAGVSAAL
                     VIHHFGSKEGLRKACDDRIADEVYGAKSESLRTSDPATWFAQVAEIESFAPMVAYLLR
                     SLQSGGELAKTFWNRMIDDAERYMEEGVQAGTLKPSRDPRARAKYLGMSSGGSLLLYL
                     QMHDNPTDLRAVLRDYGEEMILPALEVYTNGLMTDSTMYDAFVDAREEGIPLTTTQEG
                     EQNDGDVPAAG"
     gene            complement(17360..18019)
                     /locus_tag="VFC01_01575"
     CDS             complement(17360..18019)
                     /locus_tag="VFC01_01575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041310272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HZN78365.1"
                     /translation="MAGMDDVIGPQSRADSIVTHAEGSISEDAIVAGARERAVDIGAG
                     AVTPAVGALLCVLAKVTGAKAVVEVGTGAGVSGLWLLSGMREDGVLTTIDVEPEHQRL
                     AKQAFTEAGVGQSRTRLIVGRAQEVLTRLADESYDLVFIDADPIDQPQFVAEGVRLLR
                     SGGAIVLHRAALGGRAGDASAKDAEVSAVREAARLIAEDERLTPVLVPLGDGLLVAAR
                     D"
     gene            18198..18959
                     /gene="sigE"
                     /locus_tag="VFC01_01580"
     CDS             18198..18959
                     /gene="sigE"
                     /locus_tag="VFC01_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigE"
                     /protein_id="HZN78366.1"
                     /translation="MEHGGRWRSGNKHVDKRVAVDIEPSIRDGGCDQEDPTTTTVTTP
                     AAPVSMAHLPQYTEGDWVETSDELTGTAVFDATGDATTMPSWDELVRQHADRVYRLAY
                     RLSGNQHDAEDLTQETFIRVFRSVQNYQPGTFEGWLHRITTNLFLDMVRRRGRIRMEA
                     LPEDYDRVPADEPNPEQIYHDSRLGPDLQAALDSLPPEFRAAVVLCDIEGLSYEEIGA
                     TLGVKLGTVRSRIHRGRQALRDYLASHEHDTAKSA"
     gene            19045..19440
                     /gene="rseA"
                     /locus_tag="VFC01_01585"
     CDS             19045..19440
                     /gene="rseA"
                     /locus_tag="VFC01_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma E factor RseA"
                     /protein_id="HZN78367.1"
                     /translation="MVDPGHVFRRAFSWLPSQFASQSDAPVGPRQFGSTEHLSIEAVA
                     AFVDGELRMTAHLRAAHHLSLCSECASEVEAQRQARSALRDSCPIVVPSSLLGMLSQI
                     PHHAPPEPPDQADQPQLADGTPRYRRKRR"
     gene            19510..21003
                     /locus_tag="VFC01_01590"
     CDS             19510..21003
                     /locus_tag="VFC01_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN78368.1"
                     /translation="MTNLDQSGRERLEPRPVSRPSVDPASRRAFGRPDGVNGSFVGLD
                     RYRDQGEYTPKDQAPDTVLAEAFGRPYAGGDSLQRHPADSGALDAEREGPGTGEADDP
                     WRNPGAPAALGTPALPQSAPAPSAAAMGKLGVRDVLFGGRVSYVALAVLAITALVIGV
                     AGGWVGRKTAEVVEAFTTSKVTLETNNNSEPPQGQFAKVAAAIADSVVTVEAVSDSEG
                     SQGSGVVVDGRGYIVTNNHVISEAAANPSQYKTTVVFNDGKEVPANLVGRDPKTDLAV
                     LKVDNVDNLTVARMGDSEKLKVGDEVIAAGAPLGLRSTVTHGIISALHRPVPLSGDGS
                     DTDTVIDGVQTDASINHGNSGGPLINMNSEVIGINTAGKSLSDSASGLGFAIPVNEVK
                     QTVETLIKDGKIAHPTLGLTARSVSNDIASGAQVANVKAGGPAEKGGILENDVVVKVG
                     NRTVADADEFVVAVRQLAIGQESPIEVVRDGRHVTLMVTPGADTPTT"
     gene            21003..21401
                     /gene="tatB"
                     /locus_tag="VFC01_01595"
     CDS             21003..21401
                     /gene="tatB"
                     /locus_tag="VFC01_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HZN78369.1"
                     /translation="MFANVGWGEMLLLVIAGLVILGPERLPGAIRWTSGALRQARDYI
                     SGATSQLRDDLGPEFDDLREPLSELNKLRGMSPRAALTKHLLDGDDSFLTGKFDKPES
                     SPEKPVSSPAPQPVAPSTPPATTPFDTDAT"
     gene            complement(21405..22286)
                     /locus_tag="VFC01_01600"
     CDS             complement(21405..22286)
                     /locus_tag="VFC01_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78370.1"
                     /translation="MDADQVIRIALTVITPILTAGIGIAALVIGDWRDRRTQAGRRKL
                     ALEDASRQVAFAADWWNASKLIANSPDAEQQAATRAQAWLEEASALVVESKPPPVDEK
                     PAITFRRLLLAYPMERRGARILRALYYCCLGVVAFQVSGAMASAMGRPDTLGIPDYFS
                     GGLIYGDLMAVAAVTVIAMALRFWSLRVEKSQPTAQPRSGLTFRRALLLYRFHRPAAS
                     IARIIFWFWAALAILVVIAAVLGAFDEPRLIPASLIGLVAWVGWAIGLRYWAASLNER
                     TATNANRASSDPTAPGD"
     gene            complement(22338..23483)
                     /locus_tag="VFC01_01605"
     CDS             complement(22338..23483)
                     /locus_tag="VFC01_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZN78371.1"
                     /translation="MSQSPSELESAVRASLAKVIDPELRRPITELGMVKNVTVDGGDG
                     AVHVEVYLTTAACPKKTEISERVSRAVTDVPGTGAVKVSLDVMSDEQRTELRKQLRGD
                     SREPVIPFAQPGSLTRVYAVASGKGGVGKSSITVNLAAAMAARGLSVGVLDADIYGHS
                     VPRMMGTTDRPTQVESMILPPVAHDVRVISIAMFTQGNTPVVWRGPMLHRALQQFLAD
                     VYWGDLDVLLLDLPPGTGDVAISVAQLIPGAEILVVTTPQLAAAEVAERAGAIALQTR
                     QRIVGVVENMSGLLLPDGTTMQIFGEGGGKQVAERLSRAVGADVPLLGQVPLDPALVS
                     AGDDGLPLVLSAPDSAAGKELRNVADALSSRKRGLAGMSLGLDPSGR"
BASE COUNT         4165 a         7846 c         7777 g         3706 t
ORIGIN      
        1 gatactccag cacccggcgc gccgggccgg gcagcgtcgc gacgaactct tcgaccatcg
       61 tggcctgcct gctgtcgtcg tggccagccg gcgcgacatc gatccgggcc aacaggtcgt
      121 cgcgccacag cgccgaaatc ccgtcgggga caggctcgtt cggcgcgagg gtgacgatca
      181 gcttcaccgc gccgctgacc gccagctggt agacgagcgc cgcggacagc ttgtccagca
      241 gatgcgcgtc gtcgacgatc agtagcctgc cgtcgctcag cgactcccgc gcagcgcgca
      301 gcacggccgc cgtcttctcc gtgtcggaca cctcgatgag gtggttgaag ggagcgaagg
      361 gcaccgcagg cgcggtcgcc gtcacccagt cgacgcgacc gaactgcgac gccaattggt
      421 ctgccgccgc gcgtgccaac catgtcttgc ccacacccgc ggcgccgatc agcactgtcc
      481 ctgcctggtc gcggatgccg gtttcgatga gttccagtgc cggttcgtgc ggcgcggtca
      541 cccactcaac aggcaccgcg cgagtctatg gctacgttgt cggtgtgtcc ccagaacctg
      601 agcgcgaatg ggcggtctgt gtgtactgcg cgtccgggcc aacgcatgcg gagctgttgg
      661 ccttggccgt tcaagtcgga gaagcgatcg ccgagcgcgg ctggacgctg gtgtccggcg
      721 gcggcaacgt ctccgcgatg ggcgcgttgg ccggcgcggc ccgcgcccgc ggcggctaca
      781 ccgtcggcgt catcccgaag gcgttggtgc accgggaggt cgccgatacc gaggccgacg
      841 agctcgtcgt cgtcgacacg atgcgggagc gcaagcaggt gatggaggac cgcgccgacg
      901 cgttcatcgc gctgcccggc ggcataggca ccctcgagga gttcttcgaa gcctggacag
      961 cggggtactt gggtatgcat gacaagccgg tggtcatgct cgacccgatc ggccactacg
     1021 acggcctgct ggcctggttg cacgggctgg tcgacagcgg ttacgtcgca cagggcgcgc
     1081 tggaccggtt ggtcgtcgtc gacaacgtcg aggtcgcgat cgaggtttgc gcacccgcat
     1141 cgtgacgctg atcacccgtg gttagggtgt gcgacaacag aacattggag gggacgcacc
     1201 gaatgaccac ccgaaggcga ccgcgagtga ggatcgcagc gcggaacgag cgaggaccgg
     1261 agcgagcggg agtcgaggca tgacggacga gaagtcggga actcgcagca gcgtcgggct
     1321 gctcgacatc gcgagccagg tacctggtct gctgatggat ttgccgacca tcttgagcgg
     1381 tgtgatcacc ggattcgggg cgcggccgtc ggccaagacc tcgatcggca aggttttcca
     1441 ggagcgcgcg gcccgctacg ccgacaaggt gttcatcaag ttcggtgacg accagctcac
     1501 gtaccgcgag gccaatgagg cggtcaatcg gtacgccgcg gtgctggccg cccgcggtgt
     1561 cgggcacggc gatgtcgtcg gcatcatgct gcgcaactca ccgcagtcgg tgctgttgat
     1621 gttggccacg gtcaaatgcg gtgcgatcgc cggaatgctc aactaccacc agcgcggcga
     1681 cgtgctcaag cacagcttgg ggctactgag cgccaaggcg gtggtcgccg agacggagtt
     1741 catcgaaccg atcaccgaca gcggcgccga tacctccggc gtgatgaccg tcgacgagct
     1801 gcaacggctg tccgcgaccg caccgaccgg caatccggcg acgacggcgg ctgtgctggc
     1861 caaggacaag gcgttctaca tcttcacgtc gggcaccacc ggcatgccga aggccagcgt
     1921 gatgacgcac taccggtggc tgcgcgcgct cgccggattc ggcggcctcg gcatgcggtt
     1981 gagcagcaac gacaccctgt actgctgcct gccgctctac cacaacaacg cgttgacggt
     2041 cgcgctgtcg tcggtgctga actcaggcgc gacgctggcg ctgggcaagt cgttctcggc
     2101 gtcgaagttc tgggacgagg tcatccgcta cgacgccacc gcgttcatct acatcggcga
     2161 gatctgcgcc tacctgctca accagccgcc gaaggacacg gatcgcaagc acaaggtgcg
     2221 ggtgatcgga ggcaacgggc tgcgccctgc catctgggac gagttcaccg agcggttcgg
     2281 catcgaacgt gtctgtgagt tctacgcggc cagtgagggc aacaccgcgt tcgtcaactt
     2341 cctgaacctc gacaagacca cggggatctg cccgacgccg gtggcgttcg tggagtacga
     2401 ccccgacagc ggcgaaccgg tgcgcgacga gaacggccgg gtgcgaaagg tgcgcaacgg
     2461 tgagccaggg ttgctgctct ccaaggtcag cagcttccaa ccgttcgacg gctataccga
     2521 caagaaggcc agcgaaaaga agttggtgcg caacgctttt cgcgacggcg acgtctggtt
     2581 caacaccggt gacctgatgc gctcgcaggg cttcgggcat gcggcgttca ccgaccggct
     2641 cggcgacacc ttccggtgga agggcgagaa cgtcgccacc acagaagtcg aggccgcggt
     2701 ctccaccgac ccgcaggtcg aggaggcgac ggtgttcggg gtcgaggtcg agggcaccgg
     2761 cggccgggcg ggcatggcgg cgattcagct caaggagggc gaggagttcg acggcaaggc
     2821 gctggcgaag gcggcttacg agcggctgcc gggctacgcc gtgccgctgt tcgtccgggt
     2881 cgtccaagag ctcgcgcaca cgtcgacgtt caagagccag aaggtcgacc tccgcaagga
     2941 gggttacgac accgaagagg tcgaggaccc gatctacgtg ttggccggcc gcgacgaggg
     3001 ttacgtcgag ttctacgacg agtacccaaa agaagtcgcc gacggaaaga agcccaagaa
     3061 ctagcgactt cggtgcgcta acagtcgctg agcgacgtgc tgagcgacgg taagcgcgcc
     3121 gaaatcgcag gcccgtaacc tggaaggcgt gttgtcgacc ttctgcggcc gcccggtggc
     3181 cggcgatcgc gccttgatca tggcgatcgt caaccggaca ccggactcgt tctacgaccg
     3241 cggcgccacg ttcaccgacg aggccgcgaa gacggcggcc caccaggtga tcgaagaggg
     3301 cgccgacgtc gtcgacgtgg gtggcgtcaa ggccggaccg ggcgacaccg tcggcgtcga
     3361 tgaggagatc gcccgcgtcg tgccgttcat cgagtggctg cgcgacacct tccccaacca
     3421 gttgatcagc gtcgacacct ggcgttcgga ggtcgccaag caggcctgcg ccgcgggcgc
     3481 cgacttgatc aacgacacct ggggcggcca cgacccggcg ctggcggagg tcgccgccga
     3541 gttcaaggcc ggcctggtgt gctcgcacac cggcggtgcg acgccacgca cccgcccgtt
     3601 ccgcgtcaac tacggcatct ccgaacgcgg cgtcgtcgac gacgtgatcg cggaggtgac
     3661 agccgccgcc gagcgcgccg tggccgtcgg agtggcgcgt gacgcggtgt taatcgatcc
     3721 gactcatgat ttcggcaaga acacttatca cggtcttagt ttgttgcgcc acgtaaaaga
     3781 ccttgtaaaa actggatggc cggtcctgat ggcgctgagc aacaaagact tcgtcgggga
     3841 aactctgggt gtgggtctga ccgaacgcct ggagggcacc ctcgcagcga ccgccctggc
     3901 cgccgcagag ggtgccgcga tgttccgggt gcatgaagtc ggccccacgc gacgcgtact
     3961 cgaaatggtc gcgtcgattc agggcgtgcg tccgccgacg cgcacggtga ggggactggc
     4021 atgacactga gacgaacgcg agtgaggatc gcagcgaggg acgagcgagg accggagcga
     4081 gcgggagtcg aagcatgaca ctgacacccg agctacccgg aacggacgtc gtcaccggga
     4141 gatggctaac cgatcacagc tggaaccggc cgacatggac ggtcgacgaa ctgatagccg
     4201 ccaaggctgg acgcaccatc tccgtggtgc tgcctgcgct caacgaggaa gaaacggtcg
     4261 gcagcgtcgt cgagacgatc accccgctgc tcggcggtct ggtcgacgag ctcatcgtgc
     4321 tggactccgg ctccaccgac gacaccgaaa tccgcgcgct cgccgcgggc gcccgggtga
     4381 tcagccgcga ggtcgcgctg cccgaggtcg cgccgcagcc cgggaagggt gaggtgctgt
     4441 ggcgatcgct ggccgccacc gccggcgaca tcatcgtgtt cgtcgactcc gacctgatcg
     4501 acccggaccc gatgttcgtg ccgaagctgg tcggcccgct gctcaccacc gacggtgtgc
     4561 acctggtcaa gggcttctac cggcggccgc taaaggtcag cggcagcgag gacgccaacg
     4621 gcggtggccg ggtcaccgaa ctcgtcgcgc ggccactgct cgcgtcgctg cgacccgaac
     4681 tgagctgcat cctgcagccg ctgggcggcg aatacgccgg cacccgcgag ctgcttacct
     4741 ccgtgccctt cgcgccggcg tacggggtgg agatcgggct gctgatcgac acctacgacc
     4801 ggctcggcct ggacgccatc gcccaggtca acctcggtgt gcgcacgcac cgtaatcgcc
     4861 cgctgaccga gctcgcgtcg atgagccgcc aggtcatcgc caccctgctg tcgcgttgcg
     4921 gcgttccgga ctccggcgca gccctgacgc agttcttcgc cgacggcgac gactacaccc
     4981 cgcgcacgtc ctcggtgtcg ctcgaggacc gcccgccgat gatcacactg cgcccacgcg
     5041 gctaattgac ctgacggccc cagcggggga cgatcagcga ttatgtgggg gatgaacaaa
     5101 cggtagcgac gtgacggtcg acgtcgagta cgcccgccgg ggggacatca gcatcgccta
     5161 tcagacgatc ggcgatgggc ctgtcgacat catctttggt gccggcctgg tctcgcatct
     5221 cgatctgttg tgggcagacc cgtatgcgac cgcgttcttc cggcggctgg catcgatcgg
     5281 acgcctgctg ctgttcgaca agccgggcac cgggctgtcc gatccggtcg tcggtattcc
     5341 gacggtgcag cagcgggtcg acgattttct cgccgtgctc gacgcggccg gctcgcgccg
     5401 ggcggtggtc atcgggaatt cagaagcagc cacaccggcg atcctgcttg ccgcgacgca
     5461 tccggagcgc gtcgaggcgc tgatcgcgat gtgcggcgtg ccgcgtatta cgcccagcga
     5521 cgacttcctg ccgggcagcg agcaccgatg ggaacggatg tgggcgatta ttcgacactc
     5581 caccgaccat tggggcgacg ggtcgctgct ccaatctctg tccccgtatg cgcgcgagag
     5641 cgcggtgtac cgcaggtttg cgccgaacgt cgagcgggcc tgcgcgagtc ccggcatggc
     5701 gcgggtcatg ctcaatgcac tgtgggatta cgacgtcacg ggcgcaaccc acgcggtcga
     5761 tgtgccgacg ctggtggtcc accggaccgg cgacatcctt cccatcgagg ccgcacggtg
     5821 gacggccgac gccatcgccg gggccaagct gctcgaacta cccgggcccg agcccgtgtg
     5881 cttcttcaac ggtgacgacc ttctcgccgg cgtcgaggac ttcgtcggcg gcagcgcacc
     5941 ccgagggccc atctcacgca agctggtcac cgtgctttac accgacatcg tggagtccac
     6001 ggcgcgtgcg gtggaactgg gtgacgagcg gtggtcggcc ctgctggacg cgcatcacgc
     6061 cgcggtgcgt gatcaggtcg agcgccacga cggcacgctg atcaagacga tgggcgacgg
     6121 catgcttgcc acgttcgacc gcccgacact cgcggttcgg tgcgcgctgg ccatctcccg
     6181 gcatgcgggc gaggaaggag tgcaggtgcg ggcgggggtg catgcgggcg agtgcgaagt
     6241 cagcgaccat gacatcagcg gtatcgccgc ccacatcggg tctcgcatca tggcgctggc
     6301 cgggccggcc gaggtgttgg tatcggcgac ggtgcgggac ctggtgttcg gctcgggcgt
     6361 cgagttcgaa gcgcgcggcg agcacgagtt gaagggcgta ccgggcaggt ggtcggttca
     6421 tcacgtcgtg gccgaccgcc gcgaggacca gcggcctgcg ttccaggcca cgcccgagct
     6481 ggcagcgttg acgccgtcgc cgatgacgac gatgaagccc gccgaccggg caatcgtcgg
     6541 ggtggcgaat cgggcaccgc gcctgagccg tttcacgctg cggatgctca gccgccgtcc
     6601 ggtctcgaca aggggctgat tcgacgcgtt gtcggcccct tcgggcagta tcgacagcgt
     6661 gaccttggtt ctgctgtacc tggtagtgct gatcctggtg gccgtcgtgc tgttcgggct
     6721 cggcagcgtg ctgttcggcc gcggtgagtc gttgccgccg ctgccgcgcg ccaccaccgc
     6781 caccgtgttg ccggcctcgg gcgtcaccgg cgccgatgtc gatgcggtca agttcaccca
     6841 gacggtgcgc ggctacaaga ccagcgaggt cgactgggtg ctggaccggc tcggccagga
     6901 gcttgacctg ctgcgcggcg agctcgccgc agtgcgcgcc gcgcacggca tcgacgacga
     6961 ctacgaggat gccgacgagc cggtcgaagg tggcgcgcat gcgttgccca atggcgagga
     7021 cgattcgtga ccgtcgacga cggccgcacc cggtgcggtt ggatcgacca atcacgtttg
     7081 gccccagccg atttcgtcct ctatcgggac taccacgaca cggagtgggg tcagccgctg
     7141 cgcgattcag ctgcgctgtt cgagcggatc agcctggagg cgttccagag tggcctgtcg
     7201 tggctgatca tcctgcgcaa gcgggagaac tttcgtcgcg cattccacgg gttcgacgtt
     7261 gaccgcatcg cccgctacac gaaacgcgac atcgaccggt tgatggccga caccgggatc
     7321 gtgcgcaacc gcgccaaggt cgaggccact atcgccaacg cccgcgccgc cgccgatctc
     7381 gacgtcgacc tttccgagtt gctgtggtcg ttcgcgccgc cgccgcgacc gcggcccgcc
     7441 gacctgtccc aggttcctgc ggtgacaccg gaatcgaccg cgatggccaa ggaattgaag
     7501 agacgtggtt tccgattcgt cggaccgacg acggcctacg cgttgatgca ggccaccggc
     7561 atggtcgacg atcacactgc cgactgctgg gttccagcga tcaaagccgc atcgtaacgg
     7621 cggtcatagc cgggtctttg cacacgtagc gcggcggata gggaacaata ggggaagatc
     7681 actagcccga gcgggccagc tcatgtggag ggagcactcg atggcggcga tgaagccccg
     7741 gaccggagac ggtccactgg aagcaaccaa ggaggggcgc ggcatcgtga tgcgggtacc
     7801 actggaaggc ggcgggcgac tcgtcgtcga actcaccccg gacgaagcgg ccgccctcgg
     7861 cgacgagctc aagggcgtca ccagctagcg atctcggtgc gcttacgtac gctcagcgca
     7921 cgtttccgca ccgaaatcta cgaagagacc tttcggtaga tctccaaagt ctgctcggcg
     7981 atgtgcgccc acgagaactc ttggatgcag cgctgcctgc cggcttcgcc gtactgacgc
     8041 gcccgctcgg ggtcggccac caacgagttg accgcctcgc caagccgccg ctcgaagacc
     8101 gccgggtcgc tcgcgtcgta gtgcacaagc agacctgtct gacggtccac gaccacctcc
     8161 ggtatgccgc cgacatcgga ggccaccacc gccgtcgcgc acgccatcgc ttccaggttc
     8221 acaatcccca acggttcgta caccgacgga cacacgaaaa cggttgccgc ggagagtatt
     8281 tcgcgaatct tgccgatggg cagcatctcg cgaacccaga acacgccgct gcggacgcgg
     8341 gccagctcct gcaccgccga cgtcacctcg gccgcgatct ccggggtgtc cggtgcgccc
     8401 gcacacaaca ccagctggat ctcaggcgcg aagtagtggg ccgccgcgac caggtgcgcg
     8461 acccccttct gccgggtgat ccgcccgacg aaggcaacga tcgggcgatt caggtcgacg
     8521 ccgagctctt cgagcaccga gtcgcccggc tgcggctgaa cgggacacca aacgtcagtg
     8581 tcgatcccgt tgcgcacaac gtgtacccga ttggggtcca gcgccgggta ggtgtgcagc
     8641 acgtcgtcgc gcatgccgga actcactgcg atcaccgcgt cggcggcctc gaccgccgtc
     8701 ttctccaccc acgacgacac ccggtaaccg ccgccgagct gctcggcctt ccacggccgc
     8761 atcggttcca gcgaatgcgc ggtcagcacg tgcgggatgc cgtacagcag cgcggccagg
     8821 tgcccggcca ggccggtgta ccaggtgtgc gaatgcacga ccgtggcgtt cgacgcgccg
     8881 ttgaccatgt tgaggtccgc ggacagcgtc gacagcgccg cgttggcgcc cttgagcgcc
     8941 ggatccggtt gcgccacgat cacccccgga cgcggggcgc ccatgcagtg cacgtcgacc
     9001 tcgcaaaggg tgcgcaactg cgcgacaagt tcggtgacgt gcacgcccgc gccgccatag
     9061 acctcgggtg gatactcccg agtcatcatc gccacccgca tagttccgac cgtagtagcc
     9121 atcgcaagcc gccgcagcga ttgttttgga cgcggcgacg ccaattacct tgacggacag
     9181 cccgtcagaa gtttgatcaa tgggacgaat ggctagccga gttcagtacc ggccgatagg
     9241 tttgaacgat gcgggaattg ccacatgtgc tgggcatagt cctggccggc ggagagggca
     9301 agcggcttta tccgttgacc gcagaccggg ccaagcccgc agtgcccttc ggcggcgcat
     9361 atcggctcat cgatttcgtg ctgtccaacc tggtgaatgc tcggtacctg aggatctgcg
     9421 ttctcaccca atacaagtcg cattcgctgg accgtcacat ttcgcagaac tggcggcttt
     9481 ccgggcttgc cggcgagtac atcacgccgg tgccggccca gcagcggctc ggcccgcgct
     9541 ggtacaccgg ctctgccgac gcgatctatc agtcgctgaa tctgatctac gacgaggatc
     9601 ccgactacat cgtcgtgttc ggcgctgacc acgtgtatcg gatggacccc gagcagatgg
     9661 tgcaggccca cattgaaagt ggagcgggcg gcaccgtcgc cggcatccgg gtgccacgtg
     9721 ccgaagccac tgcgttcggc tgcatcgact ccgacgaatc cggccgcatc cgcggcttca
     9781 tcgagaaacc ggccgatccg cccggcacac ccgacgaccc cgagcagacg ttcgtatcga
     9841 tgggcaacta catcttcacc acgaaggtgc tcatcgacgc gattcgcgcc gacgccgacg
     9901 acgaccactc cgaccacgac atgggcggcg acatcatccc gcggctggtc accgacggca
     9961 tggccgccgt ctacgacttc aataacaacg aggtgcccgg cgccaccgaa cgcgatcacg
    10021 gctactggcg cgacgtcggg acgctcgacg cgttctacga cgcccacatg gatctggtgt
    10081 cggtgcaccc ggtgttcaac ctctacaaca agcgctggcc gatccgcggc gaggcggaga
    10141 tgctggcgcc ggcgaagttc gtcaacggcg gctcggcgca agagtctgtg gtcggcgcgg
    10201 gcagcatcat ttcggctgcg tccgtacgca attcggtgct gtcctccaac gtcgtggtcg
    10261 acgacggcgc gatcgtggag ggcagcgtga tcatgcccgg cacccggatc ggccgcggcg
    10321 cggtggtgcg ccacgcgatc ctggacaaga acgtcgtcgt ggggccgggc gagatggtcg
    10381 gcgtggacct ggagaaggac cgcgagcggt tcgcgatcag cgccggggga gtcgtcgcgg
    10441 tcggtaaggg cgtctggatc taatcctccc ctagctccgc tgtgggccca cccctacctt
    10501 cggtgtgggc ccaagctgcg ctgtgggccc aggtttgcgg ccttgcgtcg tctgcgacgc
    10561 acgatccgca ccacggccca gatgagcacg acgacggcca ccagcacgag caccggtacc
    10621 agaaccgcca ggaacaccag cccgacgctg aagacgtcct caatcgtgga tagcaccggc
    10681 gcggccacgc ccgcggtcgc gacgttggcg gcaggccgca ccgtcgactt cgtcagcgac
    10741 accaccagcg cggtcaccac gccgatcgcg acgggaatcc actgaccgga ctgcgcgaac
    10801 gcgccagggt cggtcaccgc ggacgtctgc gccgcagtcc ccgacccgaa cacgatgccg
    10861 ccggccgtcg ggcgaacaaa cgtctggatg aggtcgttga cgctgtcgag cgccgggatc
    10921 ttgtcggcga cgatctcgac gacgagcagc acggcgacga tcgtcatcac ccagccgttc
    10981 tccagccagg cccaaccgtg cggcagcgtc accaggtccg tgaaccgcga cagcaggccg
    11041 agcgccagca gcgggatata ggcgttgagg cccgccgcgg tggccagccc gaagccggtc
    11101 agcagttcca catacgcatt atctgtcgag caaagccaaa ctgaagccac gcagccgcgt
    11161 cggttcggca tcgatggtta tctcggagat cttgtcgccg acgatggcga agcgcagcac
    11221 cagcgccagc tttcccttgg gggcgacgac gatgccgacg gcgccgtcca ccaccgcagg
    11281 ctcggcgaac cgcgcattgg ccgagaaggc acgcgcattg gcggccaccg cctgcgcacc
    11341 gcggatcgtc gtcggggtgc cggccgcggc ggcgtcgaca tgcagcacca cgtcgctgtc
    11401 gaggaccgcg agcagcgcgt cgaaatcacc atcgcgcgcg gccgtcagga acgcgtcgac
    11461 aagtcggcgt tgatggacca aatccgttgc ggggtcgggc gttttgccgc gaacgcggcg
    11521 acgggcacgc gctgcgagct gtgcggcgct gttctcggtg cggcccacga tcggtgcgat
    11581 ctcggcgtac ggcaggtcga acatgtcgtg tagcacgaac gcgagccgct cggccggatt
    11641 caaggtttga aggatgacga gcagcgcgac gccgatggag tcggcaagca ccgcctcggc
    11701 ttcgggatcg acggcgtcgg gcgcggcgac ggccggagcc ccgtcgagcg gctcctcgcg
    11761 gcgcgcgctg cgggtccgca gcatatcgag gcagatgcgc gccacgaccg tcgtcaacca
    11821 gccgcgcaca ttgcggacgt cgctgatgtc ggtgcgctgc atccgtagcc acgcttcctg
    11881 gacggcgtcg tcggcctcgg tcagcgagcc gagcatgcgg taggccacag cgcgcagatg
    11941 cgaacggtgc tcttcgaagg actctgcagg catgtcgcac ctcctcccgt catggtgatg
    12001 acggatcagc gggcgcggat atgacagtca ccagaagccg ggcaccaacc ggtagtgcac
    12061 cttgtgggtg tactcgtcgt atccggcgag ttcctgccga agcatcttct cctcgtcgtc
    12121 gatgcggagg aggagcaccg ccacagcggg cacgatagtg accagacccc aataggagtc
    12181 gagcgccagc ggcatgccca ccatcatgat caacgcgccg gcatacatcg gatgccgcac
    12241 aaggccgtac agaccggtat cgacaaccgg ctgctcggcc tcgaccgtaa tcgtggcggc
    12301 cgcgtagttg ttctgctgga cgacaagcat cgacatgcca agaccgatgg cgaccaacac
    12361 gtcgccgacg atgacgatcg gcgtcgggac ctgtgaccaa ccgaatcgat ggtcgagggc
    12421 gctgaggacc agcaccgcca gcaccatcgc ggcgatgccc acggtggcga tcttctgcac
    12481 aggcctggtc tccgcggtcg gccccgaacg catgcggcgc cgcagaatat cgggtcggcg
    12541 cagcgcccag tacacgctgg gaccgaacga cgtcacgccg aagaccgcga tgaaaaccca
    12601 tgcctgccaa tagttgaacg tgccagcagg ccagaacagc ggcacagcga agaaaaccaa
    12661 tccgaatagg ctcgccacca cggcttgaag caagagcttc acgattcctc catcaccaga
    12721 cgcccggcat cagccggtag tgcaccttgt ttgtgtagtc gggatagccc gcgagttctt
    12781 gggtgagcac cttctcctca tcgaggatcc gaagggcgag gatggccacg ccggggatca
    12841 cgaagacgag tccccaatac gagccgagcg ccagcgggat gccgaccatc agcacgacgt
    12901 ttccgaggta catcgggtgc cgaacaacgg agtagagacc cgtcgacatc acctgttgac
    12961 cggattcgac cgtgatgtta gccgacgcgt agccgttctg gatcgtcgtg agcattgcga
    13021 tacccaggcc gacggccacc aggacatcgc cgacgacgga gatggtcgcg ggcaccgagg
    13081 accagccgaa gcggaagtcc aaggcgctca ccacaattgt ggcacccatc gccaggaatg
    13141 cgatcgagat gatgaccttc tgcagtaccc gtgtctctgc ggccgggccg gcctgcatcc
    13201 gacgcttgag tgccgcggga ttcgtgaccg ccaggtaaag gctggggatc gtcgtcgacg
    13261 cagcgaacac cacaatgaag gtccatccct gccagtagtc gaatgtgccg gccggccaga
    13321 acaccagcag cccgaataca acgaggccga tgaggccgga agtgattgcc tgcaaggcga
    13381 ttttcatggt tctccttcac cagacgtgcg gcaccaggcg cgaatgcacc ttctcggtgt
    13441 actcgcggta gccggtcagt tcttccgtca gcgccttctc ctcatcgagg atgcgagcgg
    13501 cgaggagaac caggtcgaca aggactatga cgaccgccca ataggaaccc agcgcaaagg
    13561 gcatgccaat gagcatgacg agacctccga agtacatcgg gtgccgcacc agcccataga
    13621 gcccggtgga caccaccgtc tggtctgcct cgacggtgat attcgctgcg gcatagctgt
    13681 tttggacgac cacgaccatc gtgatcagaa gcccaacggc ggccagcacg ttcccgacga
    13741 gcgccaccgt ggttcctacg ttcgaccagc cgaaccgatg gtcgaacccg gacaccacca
    13801 gcaaggcaaa gaaggtcaac accacgccgg aactgacgac cttctggacg ggcctggact
    13861 cgtgggtcgg gcctgcattc atccggcgtc gaaggacttc cggattcttc acacccacat
    13921 aaacggtgta gatcaccgac aggacaacga atatgccgag gaagacccac gcctgccagt
    13981 agtcgaacgt gcccgccggc cagaacaacg caaggccgat caacgccaag ccaagcagcg
    14041 acgttgttgc tatttgaagt ccgatcttca tgatctcttc tcctacagcg cgtcgcggct
    14101 gcgatagatc cagccggcga ggattgtcag cgccgccgcg gcgacgacca tgaccgccag
    14161 cgcaacggca gggaagtcgg tcggcacggt ggtgcggccc accggtgaca ggtcgagcag
    14221 ccaccgcggc aaccgcaaca gcgctccgag gtacagcgac accacaacga atgtgactgc
    14281 aagccaaccg atccacgcgt gcttcaccgc gacggcgaat gccgcaacgc cggcgagcac
    14341 cgccatggcg ggcacatagg ctaggcctgc caacgtcaac ttcacgatgg ttccgggctc
    14401 gccgagcgta agcccggcgc cgaggccgtt gccaagcccg gcgaagaaca tcagcacgat
    14461 cgaaccgacc agcgcggacg ccaccgccgt cagaagccaa cgccagcgcg acaccgcgcc
    14521 ggccaacacg gcctcgccga gccccgactg ctcatccgaa tacagccgca acacagcggt
    14581 gacgacgtac gccgtggcgg ccgcagccag gaactgcgtc atcgtggtgt agacaccgtc
    14641 ggtgccctgc gccgtcagca ctcgtgcaag cagtgggttg tccttcgccg cgtccagcaa
    14701 cgacttggtc atcgagccga atgccaagcc gcccaggaac agtccgactg tccagccgat
    14761 ggtttgaccg cgctgcaggg tgaggtgcag gccgaacacg ccggtgatcg gacgcgcgtt
    14821 cggcttctcg ccggtggatg ggatgttgcc gtcgtcgtac tgccgcctgc tttccaacca
    14881 ggccgccagc gccatcagcg cgaccgcgag tgccaccagt agagcgaacg gccaccagcg
    14941 caggtcgacg aacgcgcgca tctgttgcgc ccatgcgatg ggggagaacc agctcagcgc
    15001 gctgccggag ttgttgatga cgtcaccgat gccgcggacc agcgcggccg ccgccagtgc
    15061 ggccatcgcc gccccggtcg cggtccgggc ctgtcgccac agctgcgccg tgaccgcggc
    15121 taccgcgccg aacaccatgg ccaccccggt gacgccgagg cacatccccg cagtatcgac
    15181 gatcgcaaat cccgtcgagg ccatcgccag cgtcatggtg accgccagga cggcgttcac
    15241 cccgccgacg aggatcagcg cggcgtacgt gcgggcgtag cggccgacaa ccgacgacga
    15301 caccagttcc gcgatgccgc tctcttcctc ggcgcgcgtg tggcggatga cggtgctgat
    15361 ggaaaggatc gacgccgcga cgatcagcgt gagcattagc tcgttcgcca tcatcgcgcc
    15421 gaggtccgtc tcgttgccac caaacatggg cccgcccaac atcattccgg cgggcgtttt
    15481 gagcaggttc acccgggcca gccgttgcgg ctcttccggg tacgcgagtt ttaccgcgtt
    15541 cggggcgtac accatcatca gcgtcaggat cgcgatccac acggacagcc ggatccggtc
    15601 gcggcgcagc gcgagtcgca gcaggctggg aacgccagtg aatggcgatc ccggctgggc
    15661 cgcttcgtga cgccccgctc gcggtgcgac cgttgccgtg gtcatcgcga cgccccctga
    15721 tactcacgca ggaacatgtc ctccagcgac gcgggagtga cgatgagctc ctcgatgccc
    15781 agatcggtga ggtgctccat cgtgaagtcc aagtcgttgc ggtccaccga gaacctgact
    15841 tgcccgttgt ggctggtgaa gtcgtggacg tacggaacct gctgcagcgc ttgaccgtcg
    15901 ccgcgggtgc gaaccgtcac ggtagtgcgc atcagatgtc gcagctcgtc gagcgtgccc
    15961 gacttgaccg cccgcccgga ccggatgatt gtgactgcgt cgcaaagctt ttcgacttcg
    16021 gccaggatgt ggctggacag cagcacggcg gcaccgcgat ctgcgacctc gttgacgcac
    16081 tgctgaaaag cctgctccat caacgggtcg aggccagagg tcggctcgtc gaggatatac
    16141 agctcggcgt tggtgctgaa cgcggcgacg atggccacct tctgccggtt gcccttggaa
    16201 tacgtgcgcg ccttcttgtg cgggtcgagt tcgaaacgct cgatcagctg atcgcggcgt
    16261 cgggtgtcga caccattgcc gcgcaagcga gccaaaaagt cgatcacctg aaggccggtg
    16321 aggttgggcc acaacgtgac atcgccgggc acgtaggcga tccgccggtg cagcgcgacc
    16381 gcgtcgcgcc agggatcacc gccgagcagc cggacagtgc cgcggtcggc gcgcagcagg
    16441 ccgagcagca cccggatggt ggtggacttg cccgcgccgt tgggcccgag gaagccggcg
    16501 atttcgccgt gggccacggt catatcgagg ccgtcgagtg ccttggtacg gccgaaagat
    16561 ttgtgcagat cccggatttc gacagcaggt gaactatccc gccgcgggga cgtcgccgtc
    16621 attttgttct ccttcttgag ttgtggttaa cgggatgcct tcttcacgcg cgtcaacgaa
    16681 tgcgtcgtac atggtcgagt cggtcattaa gccgttcgtg tagacctcaa gggccggcaa
    16741 gatcatctcc tcgccgtagt cgcggaggac ggcgcgcaga tcggttgggt tgtcgtgcat
    16801 ttggaggtac agcaggagac tgccgccgct gctcatcccg aggtacttgg ctcgggcacg
    16861 cggatcgcga ctcggtttga gcgtgccggc ctgcacgcct tcctccatgt atcgctcggc
    16921 gtcgtcgatc atgcggttcc agaaggtctt cgccagctcg ccgccggact gcaggctgcg
    16981 tagcaggtag gccaccatcg gcgcgaacga ttcgatctcc gccacctgag cgaaccaggt
    17041 ggcggggtcg ctcgtccgca gcgactccga cttcgcgccg taaacctcgt cggcgatccg
    17101 gtcgtcgcag gccttgcgca ggccttcctt ggatccgaag tgatggatca ccagcgcggc
    17161 ggatacgccc gctgcctcgg cgatgctccg aagcccgacg ttaaagccgt gctgccccca
    17221 ctgttcgatc gcggcgtcgc ggatccgggc gacggccgtg cgatcgtctg acgctgaacg
    17281 catgtttagt acactaaacg cgcgttcagt gcgcagtcaa gcatctaccc cgtcaggaat
    17341 ccgtaaaaat cgcgacgagt cagtcgcgcg cagcgacgag caggccgtcg cccagcggga
    17401 cgagcacggg cgtcagccgc tcatcctcag cgatcagtcg cgcggcctca cgcacggcgc
    17461 tgacctcggc atccttggcg ctcgcatctc cggcacgtcc gccgagcgcc gcccggtgca
    17521 ggacgatcgc gccgccggac cgcagcagcc gaaccccttc ggcgacgaat tgtggctggt
    17581 ctatcggatc ggcgtcgatg aacaccaggt cataggactc gtcggccagc cgggtcagca
    17641 cctcctgcgc ccggccgacg atcaaccggg tccgcgactg ccccacgccg gcctcggtga
    17701 acgcctgctt ggcaagccgc tgatgctcgg gttcgacgtc gatcgtcgtg agcacaccgt
    17761 cctcacgcat gcctgagagc agccacagcc cgctgacacc cgcacccgtg ccgacctcga
    17821 cgactgcctt tgcgccggtc actttggcga gcacgcacag cagggcaccg acggccgggg
    17881 tgaccgcccc tgctccgatg tcgaccgccc gctcgcgcgc ccccgcgacg atggcgtctt
    17941 cggagatcga accctcggcg tgcgtgacga tcgaatccgc gcggctctgc ggtccgatga
    18001 cgtcgtccat gccggccatg cccgcagcgt agagcgaacc gatgtggccc gccgaagcga
    18061 cacgcccgtg ccggagcgtg agaatcacag tccgtcgagc ccgttttgcc tggtgggaaa
    18121 atgggtagca atctttctca ggaaatgttc agtttgctca tatgccaacc tcaacccggt
    18181 cggagacggt atctcgcatg gaacacggcg gacgctggcg ctctgggaat aagcacgtgg
    18241 acaaacgtgt tgccgtcgac atcgagccgt cgatacgtga tggcggctgt gaccaggagg
    18301 atccgacgac cacgaccgta accacccccg ccgcaccggt gtccatggca cacctgccgc
    18361 agtacaccga gggggactgg gtagagacgt ccgatgagtt gaccggcacc gcggtgttcg
    18421 acgccaccgg cgatgcgacc acgatgccgt cctgggacga gctcgtgcgt cagcacgccg
    18481 atcgggtgta ccggctggcc taccggctgt cgggcaacca gcacgacgcc gaggatctga
    18541 cgcaggagac ctttatccgc gttttccgct cggtgcagaa ctatcagccc ggcaccttcg
    18601 agggctggct gcaccgcatc accacgaacc tcttcctgga catggttcgc cgacgcggcc
    18661 gcatccgcat ggaggcgctg cctgaggact acgaccgggt gccggccgac gagcccaacc
    18721 ccgagcagat ttaccacgat tcgcggctcg ggcccgatct acaggccgcg ctcgactcgc
    18781 tgccgccgga gttccgcgca gccgtggttc tgtgcgacat cgaaggactg tcctacgagg
    18841 aaatcggcgc cacactgggc gtcaagctcg gcaccgtgcg cagccgcatc caccgcggtc
    18901 gccaggcgtt gcgcgactac ctggcaagcc acgagcacga cacggccaaa tccgcctgac
    18961 gtcgcgggtg taggtcacag ctggccgcgc tacattcgag tcaacacagt tgatgcggca
    19021 tgacgaaacg agaggagctg ggtgatggtc gacccgggac atgtgttccg tcgggcgttc
    19081 tcctggctgc cctctcagtt cgcctcccag agcgacgcgc cagtcgggcc gcggcagttc
    19141 ggatccactg agcacctgtc gatcgaggct gtcgcggcgt tcgtcgacgg cgagcttcgg
    19201 atgaccgcgc acctgcgcgc ggcacaccac ctgtcgctgt gctccgagtg cgcgtccgag
    19261 gtggaagccc agcggcaagc ccggtccgcg ctgcgggact cgtgcccgat cgtcgtaccg
    19321 agttcccttc tgggcatgtt gtcccagatc ccacaccatg ctccaccgga gccgcccgac
    19381 caagcggatc agccgcagtt agctgacggc accccgcggt accgccgtaa gcgccggtag
    19441 ggtggatgcg gagtcgagca gtgacctgcg ccggccattg cgggcgctcc agtagagggt
    19501 gatgaacggg tgaccaatct ggaccagtcc gggcgcgagc gcctcgagcc gcgccccgtg
    19561 tcgcgtccat ctgtcgatcc ggcttcgcgt cgcgcgttcg gccggcccga cggcgtcaac
    19621 ggatccttcg tgggtctgga ccgctaccgc gaccaaggcg aatacacccc gaaggaccag
    19681 gcgcccgaca cggtgctggc cgaggcgttc ggtcggccct acgcgggcgg cgattcgctg
    19741 cagcggcacc ctgccgattc cggcgcgctg gacgccgaac gcgagggacc gggaaccggc
    19801 gaagccgacg acccgtggcg caatccaggc gccccggcgg cgctcggcac tccggcgttg
    19861 ccccagtccg ctccggcccc ttcggccgcc gcaatgggca agctcggggt gcgcgacgtt
    19921 ttgttcggcg gcagggtgtc ctacgtcgcg ctggcggttc tcgccatcac cgcgttggtg
    19981 atcggcgtcg caggcggctg ggtgggccgc aagacggccg aggtcgtgga ggcgttcacc
    20041 acgtcgaagg tgacgctgga aaccaacaac aactcggagc caccgcaggg tcagttcgcg
    20101 aaagttgctg cggcgatcgc tgattcggtg gtgacggtgg aggccgtgag cgattcggaa
    20161 ggctctcagg gctcgggcgt tgtggtcgac ggccgcggct acatcgtcac caacaaccac
    20221 gtcatctccg aggccgccgc caaccccagc cagtacaaga cgacggtggt gttcaacgac
    20281 ggcaaggaag tgcctgccaa cctcgtcggc cgcgacccga agaccgactt ggccgtgctc
    20341 aaggtcgaca acgtcgacaa cctgacggtt gcccgtatgg gtgattccga aaagctcaag
    20401 gtcggtgacg aggtgattgc cgccggcgct ccgctgggtc tgcgcagcac cgtcacacac
    20461 ggcatcatca gcgcgctgca ccgcccggtg ccgctgtccg gtgacggctc ggacaccgac
    20521 accgtcatcg acggcgtgca gaccgacgcg tcgatcaacc acggcaactc cggcggcccg
    20581 ttgatcaaca tgaactccga ggtgattggc atcaacaccg cgggtaagtc gttgtccgac
    20641 agcgctagcg gcctcggctt cgcgatcccg gtcaacgagg tcaagcagac cgtcgagacg
    20701 ttgatcaaag acggcaagat cgcgcatccg acgctgggac tgaccgctcg gtcggtgagc
    20761 aatgacatcg cctccggtgc gcaggtcgcc aacgttaagg ccggcggacc ggccgagaag
    20821 ggcggcatcc tggagaacga cgtcgtcgtc aaggttggca accgcacggt cgccgacgcc
    20881 gacgaattcg ttgttgccgt gcgccagttg gccattggtc aggagtcgcc gatcgaggta
    20941 gtccgcgacg gccgccacgt caccctcatg gtcaccccgg gcgccgacac tcccacgacg
    21001 taatgttcgc caacgtgggc tggggcgaga tgctgctcct ggtgatcgcc ggtctggtga
    21061 tcctcgggcc cgagcggctg cccggcgcca tccgatggac gtcgggcgcg ctgcgacagg
    21121 cccgcgacta catcagcggc gccaccagcc agttgcgtga cgacctcggc ccggaattcg
    21181 acgatctgcg cgagccgctc agcgagctga acaagttgcg cggtatgtcg ccgcgcgccg
    21241 cgctcaccaa acatctactc gacggcgatg attcgttcct cacgggcaag ttcgacaagc
    21301 ctgaatccag ccccgagaag ccagtgtcgt cgcccgcgcc acagcccgta gcgccttcga
    21361 cgcccccggc caccacgccg ttcgataccg acgcaacgta gccgctaatc gcccggcgcc
    21421 gtggggtcgg atgatgcgcg gttggcgttg gtcgctgtgc gctcgttcag tgaagcagcc
    21481 cagtagcgca ggccaattgc ccatcccacc caggcgacaa gcccgatcag gctggcggga
    21541 atcaggcgcg gttcgtcgaa ggcgcccagc acggctgcta tgacgacgag gatcgccaaa
    21601 gctgcccaga accagaagat gatgcgcgcg atcgaggcgg cgggccggtg gaatcgatat
    21661 agcaacagcg ccctgcgaaa cgtcaaacca ctccgcggct gcgccgtggg ttgtgatttc
    21721 tcgacgcgaa gcgaccagaa tcgaagggcc atcgcgatca ccgtcaccgc tgcgacggcc
    21781 atgaggtcgc cgtagatcag gcctccggag aaatagtcgg ggatgccaag tgtgtcgggc
    21841 ctgcccatag cggaagccat cgccccagaa acctgaaaag ccacaacgcc gaggcagcag
    21901 tagtagagcg ctcgaaggat ccgcgcgccg cggcgttcca tcgggtaggc gaggagcagt
    21961 cgacgaaacg tgatggccgg cttctcgtcg accggtggcg gtttggattc gacgacgagc
    22021 gcggaggctt cttccagcca ggcctgggcc cgtgtggcgg cctgctgttc cgcgtcgggc
    22081 gagttggcaa tgagcttgct ggcgttccac cagtcggcag cgaatgcgac ctgccgactg
    22141 gcatcctcga gggccagctt gcgtcgtccg gcttgggtcc tgcggtcgcg ccagtccccg
    22201 atcacgaggg cggcgatccc gattccagcg gtcaggatcg gcgttatcac agtcagggcg
    22261 atgcgtatga cctggtcagc atccatcgcg cctgatgatg ccaccccgga cgcgtcgacg
    22321 agatcgaaaa ccgaccgtta gcgaccggag ggatccagcc ccagcgacat gccggccagc
    22381 ccccgcttgc gcgacgacag cgcgtcggcg acgttgcgca gctccttgcc cgccgccgaa
    22441 tccggcgccg acagcaccag cggcaaaccg tcgtcgccgg ctgacaccaa agccgggtcg
    22501 agcggcacct gtccgagcag cggcacgtcg gcgccgaccg cccgcgacaa ccgctcggcc
    22561 acctgcttgc cgccgccttc gccgaagatc tgcatcgtcg tgccgtcggg cagcagcagc
    22621 ccggacatat tctccaccac gcccacgatg cgctggcggg tctgcagcgc gatcgcgcct
    22681 gcccgctcgg ccacctcggc ggcggccagc tgcggcgtcg tcaccaccag aatctcggcg
    22741 ccggggatca gctgtgcgac cgagatcgcg acgtcgccgg tgcctggcgg caggtcaagc
    22801 agcagcacgt cgaggtcgcc ccagtagacg tcggccagga actgctgcag cgcccggtgc
    22861 agcatcggcc cgcgccacac caccggggtg ttgccctggg tgaacatcgc gatcgagatc
    22921 acccgcacgt cgtgcgcgac cggcggcagg atcatcgact ccacctgggt cggccggtcg
    22981 gtggttccca tcatccgcgg caccgagtgg ccatagatgt cggcatcgag cacgccgaca
    23041 gacagtccgc gcgcggccat cgcggcggcc agattgactg tgatactgga ctttccgacg
    23101 ccgcccttgc ccgacgcgac cgcgtacacc cgggtcagcg agccgggctg cgcgaacggg
    23161 atcaccggct cgcgcgagtc gccccgcagc tgcttgcgca gttcggtgcg ctgctcgtcg
    23221 ctcatcacgt cgaggctgac tttgaccgca cccgtgccgg gcacatcagt gaccgcccgg
    23281 ctgacgcgct cggagatctc tgtcttctta gggcacgccg cggtggtcag gtacacctcg
    23341 acatgaacag caccgtcgcc gccatccacg gtgacgttct tgaccatgcc gagctcggtg
    23401 atcggccggc gcagctcggg gtcgatcacc ttggcgagcg aggcacggac ggccgattcc
    23461 agctcagagg gagattggga catcggtgtc gagt
//
LOCUS       DASRJA010000034         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2217625,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000034 DASRJA010000000
VERSION     DASRJA010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2217625"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFC01_01610"
     CDS             complement(<1..423)
                     /locus_tag="VFC01_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HZN78372.1"
                     /translation="MAEVTIPAELKPNDGRFGCGPSKVRPEQLQALAAAGDLFGTSHR
                     QAPVKNLVGRVRDGLRQLFSLPDGYEVILGNGGSTAFWDAAAFGLIDERSLHLTYGEF
                     SSKFAKATNDAPFLQDSIVVKADPGDAPAPTSDPSADVV"
     gene            529..1017
                     /locus_tag="VFC01_01615"
     CDS             529..1017
                     /locus_tag="VFC01_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZN78373.1"
                     /translation="MDRGKATALLPVYAAAMMVVGSVLAATPAQADGDQGGAVLSEIN
                     ATRAANGCGPVAADPHLTASAARQANDMLENGVQGHIGSDGSSPAQRVKDAGYASYTK
                     LGEIVFWSTGPGGTPAAAVTWWMNSPRHRAIITDCELTDAGFSAVRGGNKLTVAGDFG
                     KK"
     gene            complement(1045..1830)
                     /gene="map"
                     /locus_tag="VFC01_01620"
     CDS             complement(1045..1830)
                     /gene="map"
                     /locus_tag="VFC01_01620"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZN78374.1"
                     /translation="MVELKSNAEIDAMAAAGAVVAEALRAVVEHAKPGQTTAELDKVA
                     ADVLARHDARSPFLNYHPRWAPSPFPAVLCVSVNDAVVHGIPNGEVLADGDLVSVDFG
                     AIFRGWCGDAARSFIVGTPRAHDAALIETTDKALAAGIAAVRPGNTLGDVGHAIAQVA
                     RGAGYGLLADHGGHGIGRTMHEDPHVPNEGRPGKGMRLRPGLVIAIEPMSPRKLGAQI
                     ADGTDEYTHDSDGWTLRTASGARAAHSEHTVAVTADGPRILTN"
     gene            1969..>2357
                     /locus_tag="VFC01_01625"
     CDS             1969..>2357
                     /locus_tag="VFC01_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HZN78375.1"
                     /translation="MARTRLVRRWRRNMEVGDDTEYVDMLTTLSEGSVRRNFNPYTDI
                     DWDSPEFAVFPNDPRWVLPATDPLGRHPWYQAQPLERQIEIGMWRQANVAKVGLHFES
                     ILIRGLMDYSFWTPNGSPEYRYCLHEAV"
BASE COUNT          391 a          777 c          809 g          380 t
ORIGIN      
        1 gaccacgtcc gcgctcgggt ccgaggtcgg cgcgggcgcg tcgcccgggt cggccttgac
       61 gacgatcgag tcctgcagga acggggcgtc gttggtcgcc ttggcgaact tcgagctgaa
      121 ctcgccgtag gtcaggtgca gcgagcgctc gtcgatcagc ccgaacgccg cggcgtccca
      181 gaacgccgta gagccgccgt taccgaggat gacttcatag ccgtcaggca gcgaaaacag
      241 ttggcgcagc ccatcgcgga cacggccgac gaggttcttg accggcgcct gtcgatgcga
      301 cgttccgaac aggtcgcccg cggcggccag cgcctgcagt tgctcgggcc gcaccttgga
      361 cggcccgcag ccgaaacgtc cgtcgttggg tttgagctcg gcgggaattg tcacttcggc
      421 catgagctca agactagtaa ccgggcagaa gtaaccgaaa actggggaaa cacccgacta
      481 atcgacacat actgagactg gtcgggcttc ccgttgaggg cacgtctgat ggatagagga
      541 aaggcgacgg cgttactgcc ggtctatgcg gcggccatga tggtcgtcgg atcggtactc
      601 gccgcgacac cggcccaggc cgacggcgac cagggcggcg cggtgctgag cgagatcaac
      661 gccacgcgcg cggcgaacgg ttgcggcccc gttgccgccg atccgcacct gactgcgtcc
      721 gcagcgcggc aggccaacga catgctcgag aacggagtgc agggccacat cggttcggac
      781 ggctcctcgc ctgcccaacg cgtcaaggac gcgggctacg cgtcttacac gaagctcggc
      841 gaaatcgtgt tctggagcac cggccccggc ggtacccccg ctgccgcggt cacctggtgg
      901 atgaatagtc cgcgtcaccg cgcgatcatc actgactgcg aattgacgga cgcgggcttc
      961 tcggcggtgc gcggcggcaa caagctcacc gtggcggggg atttcgggaa gaagtagagg
     1021 cagcgaaata gcttccggcc caggttagtt cgtgaggata cggggtccgt cggcggtgac
     1081 cgcgaccgtg tgctcgctgt gcgcggcccg ggcgcccgac gcggtgcgca gtgtccaccc
     1141 atccgagtcg tgggtgtact cgtcggtgcc gtcggcgatc tgggccccca gcttgcgagg
     1201 ggacatcggc tcgatcgcga tgaccagacc aggccgcagc cgcatgccct tgccgggacg
     1261 gccttcgttc ggcacgtgcg gatcctcgtg catcgtccgg ccgatgccgt ggccgccgtg
     1321 gtcggccagc agcccgtacc ccgcaccgcg ggcaacctgt gcgatggcgt gtccgacgtc
     1381 gcccagcgtg ttgccgggcc gcaccgcggc gatgccagcg gccagcgcct tgtccgtcgt
     1441 ctcgatcaac gcggcgtcgt gcgcacgcgg cgtcccgacg atgaagctgc gcgccgcgtc
     1501 gccgcaccac cccctgaaga tggcgccgaa gtcgacggag accagatcgc cgtcggcgag
     1561 cacttcgccg ttcgggatgc cgtgcacgac ggcgtcgttg accgatacgc acagcaccgc
     1621 ggggaacggg ctcggcgccc agcgcgggtg gtagttgagg aagggcgagc gcgcatcgtg
     1681 ccgagcgagc acgtcggcgg caaccttgtc gagctcggcg gtggtttggc cgggcttcgc
     1741 gtgctcgacg accgcgcgta gcgcttcggc caccacagca cccgcggcgg ccatcgcgtc
     1801 gatctcggcg ttggacttca gctcgaccac cagatgtgac ctccgtcaca taggtgatgt
     1861 gcacggccgt cgcaccacat cgccgaagtg gggctgttca atatgcgata cctgcggtac
     1921 cgtcgttgga cattagggct caaatgtaaa cctcacggga ggcttcgaat ggctcgcacg
     1981 cgtctggtcc gacggtggcg tcgcaacatg gaggtgggcg acgacaccga gtacgtcgac
     2041 atgctcacca cactgtccga ggggtcggtg cggcgcaact tcaacccgta caccgacatc
     2101 gactgggatt cacccgagtt tgcggtgttc ccgaacgacc cccgctgggt cctgcccgca
     2161 accgatccgc tgggccgcca cccctggtat caggcgcagc cgctggaacg gcagatcgag
     2221 atcggtatgt ggcgccaggc caacgtcgcc aaggtcggtc tgcacttcga gtcgatcctg
     2281 atccgtggcc tgatggacta ctcgttctgg acgccgaacg gctcaccgga gtaccggtac
     2341 tgcctgcacg aggcggt
//
LOCUS       DASRJA010000035         4493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2215975,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000035 DASRJA010000000
VERSION     DASRJA010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2215975"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFC01_01630"
     CDS             complement(<1..185)
                     /locus_tag="VFC01_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78376.1"
                     /translation="MTKQPIAMRPPAQRLLAWGNERFGPVPILLYFLLYLAGILYARA
                     ITGDPNPSVSPLDVVAF"
     gene            complement(182..2356)
                     /locus_tag="VFC01_01635"
     CDS             complement(182..2356)
                     /locus_tag="VFC01_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN78377.1"
                     /translation="MRLKSIAIFAMSRRRLVLVTALLFLIVSGAVGADAIEKLSSGGF
                     NDPGSESVHADRQLRDTFGMAVPTLVLLVSADAPIGSPEVTAAGRELVDKLAHTQGVE
                     RVESFWTSDQLGTALQSKDGRKALIRAQLAGDDNELADRGRALAAQFGGTHGPLYVEA
                     TGYAVLLAETEDIAKQDLVKSEVIAIPLTFIALFLIFRGLVAALIPVGIGVLAIFGTT
                     FVMRVLGEITDVSIFAINLASALGLALAIDYSLLIVERYREELQGCDRPEAILAALST
                     AGRTVVFSGATVALALSALAVFPMYFLRSFAYAGICVVAIAVVGAVVVLPAILAAVGP
                     RIDALSFARQRKRVPPGTGRWHTIATVVMRRPVPIAIVSVGVLAVLAVPFASAKLMFP
                     DDRNLPPSSAAASASHEIRTDFAQTENGTLVIFADGFNGAAADYEMRLSSVPGVTAVH
                     GPDGTFQDGLRTAEPSSYEQAYFVRNGDARFTVETDVEPLSPQGEQLLRDVRAVPSPF
                     QTAVTGLGAQTYDGKEAIVHRLPVALAIIAITTFVMLMWLTRSVFLPVKAIALTILSL
                     TATFGALVFIFQDGHLRWLVGDFTITNSLNTLGPPLLFCVAFGLSMDYEVFMLSRIKE
                     EYDRLGGDSAADNTAAVARGLESTGPIVTAAAVVMAIVFVSLATSGLTTVKSLGVGLA
                     IAVLMDATIVRALLVPAFMRLAGRLNWWAPRRLQQREKVAAA"
     gene            complement(2347..>4493)
                     /locus_tag="VFC01_01640"
     CDS             complement(2347..>4493)
                     /locus_tag="VFC01_01640"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN78378.1"
                     /translation="DVREPGFGVAEQVLDELGDAVGCIVHAAATIAFNAPEAEYRRVN
                     IAGTEHALDLAQRWNVPLVHVSTAYVCGRRNGTISEHELDAGQEFSNGYERSKYLAER
                     LVTQAPVDSVILRPAIVAGDAATGAVRDFRNLYTLLKLTVEGRLRRLPGCYDATLSLV
                     PVDYVADAVVAAAELVLTGDTSILGRTFHLPGAQTLSLREVTDVLAEYPSFEIGTFVP
                     PTEFSVEDLDPIQRDYYERLGAQYTCYLDRIRTFDDTNTRTLLGLVPPATGVDYLRRL
                     LDYCLAVGYLGRPVPSISEILAKVPSPVAGVAKGRHMNRTSSVMMPFAESDLTAEFLT
                     RLLRERTGDAQVEVTAAIPTGRAVRGTLSAIVQAANPARFIGVAPLRLEFGDRPAVDV
                     MVKSKPLDSEMTTSLAAVMSTAGGDLTDAWQSCADASHFIGLHRREIGLYRDAPDAVK
                     AITPECFGTYEDAEQGVFLVMIERLDGSVDFLDAADTPEVWTTERLEAAIIGITGVHA
                     HWLGREAELLGADSWVGRDDETPGMVPLWRAMYDQYRTDFPQWVDDATYDWATAVIDA
                     IPTWWAELVAMPRTLVHNDFNPRNIALRKDGSLVAYDWELATVQVPQRDLVDLLAHVL
                     GPGARPESVDHFVEFHRRALESATGTILDADVWRRGYQLSLAELSINRLPTIALGQRW
                     LPLPWFDHLVQTTKRLADIERDRSRQRIDRCA"
BASE COUNT          805 a         1413 c         1507 g          768 t
ORIGIN      
        1 ctgaaggcga caacatcgag cggcgagacc gatgggttcg ggtcaccggt gatagcgcgg
       61 gcgtacagga tcccggccag gtaaagcagg aagtacagca ggatggggac cggcccgaac
      121 cgctcgtttc cccaggccag cagccgttgt gccggcggcc tcatggcgat cggctgtttg
      181 gtcatgcggc cgcgaccttc tctcgctgtt gcagccggcg aggcgcccac cagttcaacc
      241 tgcctgccag gcgcatgaac gccggtacaa ggagggcacg cacgatcgtc gcgtccatga
      301 gtaccgcgat cgcgagcccg acgccgagcg acttcacggt ggtgagtccg gaggtggcga
      361 gcgagacgaa cacgatcgcc atgacgacgg ccgctgcggt gacgatcgga ccggtcgact
      421 cgagcccccg cgcgactgcg gcggtgttgt cggctgcgct gtcaccgccg agccggtcgt
      481 actcctcctt gatgcgcgag agcatgaaga cctcgtaatc catcgacagg ccgaaggcga
      541 cacagaacag cagcggcggc ccgagcgtgt tcagagaatt ggtgatggtg aaatcgccga
      601 caagccaacg caaatggccg tcctggaaga tgaacacgag cgcgccgaag gtggcggtga
      661 ggctcagaat ggtcaacgcg atcgccttga ccggcaggaa cacgctgcgg gtgagccaca
      721 tcagcatcac aaacgtcgtg atcgcgatga tcgccaacgc caccggtagc cggtgcacga
      781 tggcttcctt gccgtcatag gtctgcgcac cgagccccgt gactgccgtc tggaacggcg
      841 atggcaccgc ccgtacgtca cggagcagtt gctcaccctg cggggacaac ggttcgacgt
      901 ctgtctcgac ggtgaaccgc gcgtccccgt tccttacgaa ataggcttgt tcgtacgacg
      961 agggctccgc cgtgcgcagg ccgtcctgga acgtcccgtc cggaccgtgg accgcggtga
     1021 cgccgggcac cgacgacagc ctcatctcgt aatcggctgc ggcgccgttg aatccgtcgg
     1081 cgaagatgac cagcgtgcca ttctcggtct gggcgaaatc cgtacggatc tcgtggctcg
     1141 ccgaggcggc ggccgacgat ggaggcaggt tgcggtcgtc gggaaacatc agcttggcgc
     1201 tcgcgaacgg caccgccagc accgcgagaa cgcctaccga cacgatcgcg atcgggacgg
     1261 gccggcgcat caccaccgtc gcgatcgtgt gccagcggcc ggtgcccggt ggcacccgct
     1321 tgcgctgccg ggcgaaggag agcgcgtcga tgcgggggcc gacggcggcg agtatggcgg
     1381 gcagcacgac gacggcgccg acgacagcga tcgcgacgac gcagatcccc gcgtaggcga
     1441 acgatcgcaa gaagtacatc gggaacacgg cgagggcgga cagcgccaaa gcgactgtcg
     1501 ctccggagaa gacgaccgta cgtccagccg tgctcaacgc ggcgagaatc gcctcgggtc
     1561 tatcacaccc ctggagttcc tcgcggtagc gctcgacgat cagcaggctg tagtcgatgg
     1621 cgagggccag cccgagcgcc gacgccaggt tgatcgcgaa gatcgacacg tcggtgatct
     1681 ccccgagtac gcgcatcacg aatgtcgttc cgaagatggc caagacaccg atcccgaccg
     1741 gtatcagggc ggcgaccaac ccgcggaaga tcaggaacag tgcgatgaat gtcagcggta
     1801 tcgcgatgac ctcggacttg accaggtcct gctttgcgat gtcctctgtt tcggcgagca
     1861 acactgcgta tccggtcgcc tcgacgtaga gcgggccgtg agtgccaccg aactgtgcgg
     1921 ccagcgcacg tcctcgatcg gcgagttcgt tgtcgtcgcc ggcgagctgg gcgcggatga
     1981 gtgcctttcg accgtccttg ctctgtagcg ccgtgccgag ttgatctgac gtccagaatg
     2041 attcgactcg ctcgacaccc tgtgtatgcg ccagcttgtc caccagttca cgtcccgccg
     2101 cggtgacctc gggtgaaccg atcggtgcgt cggcggacac gagcagcacc aatgtcggca
     2161 ccgccatgcc gaaggtgtcg cgcagttggc ggtcggcgtg cacggattcc gatccggggt
     2221 cgttgaaccc gccgctggac agtttctcga tcgcatcggc gccgacggct ccactgacga
     2281 tgaggaagag cagcgcggta acgagcacca gtcggcggcg cgacatggcg aagattgcaa
     2341 tcgatttcag gcgcatcggt ctatcctttg gcgactacgg tcacgttcga tgtccgcgag
     2401 ccgtttggtg gtctgcacga ggtggtcgaa ccagggcagt ggcagccagc gctggccgag
     2461 cgcaatggtc ggaaggcggt tgatgctgag ttcggcgagg ctgagctggt atccgcgtcg
     2521 ccacacatcg gcatcgagga tggtgccggt agcggattcg agtgcacggc gatggaattc
     2581 gacgaaatga tcgaccgatt ccggccttgc gccggggccg agcacgtgcg cgagcaggtc
     2641 gacgaggtcg cgctgcggca cctgcaccgt cgcgagttcc cagtcgtagg cgactaggct
     2701 accgtccttc cgcagcgcga tattgcgcgg gttgaagtcg ttgtgcacca acgttctcgg
     2761 catggcaacc agttccgccc accacgtcgg gatggcgtcg atgaccgccg tcgcccaatc
     2821 gtaggtcgcg tcgtcgaccc actgcgggaa gtcggtccgg tactggtcgt acatggcgcg
     2881 ccacaggggg accatcccgg gtgtctcgtc gtcgcggcct acccacgaat cggcgccgag
     2941 aagttcggct tcgcgcccca gccagtgcgc gtgcacgccg gtgatgccga tgatcgcggc
     3001 ttcgagccgt tcggtagtcc agacttccgg tgtgtcggcg gcatcgagga agtcgacgct
     3061 gccgtcgaga cgctcgatca tcaccaggaa cacaccctgt tctgcgtcct catacgtacc
     3121 gaaacattct ggtgtgatgg cctttacggc gtcgggagcg tcgcggtaca agccgatttc
     3181 gcgccggtgc aggccgatga aatggcttgc atccgcacag ctctgccatg cgtccgtgag
     3241 gtcgccgccg gccgtcgaca tgaccgccgc gagtgaggtg gtcatctcgg agtcgagcgg
     3301 cttcgacttc accatcacat cgaccgccgg ccggtcgccg aactcgagcc gcaacggcgc
     3361 gaccccgatg aaccttgctg gattcgctgc ctgcacgatc gccgacaacg tgcccctgac
     3421 cgctctgccc gttgggatcg ccgcggtgac ctcgacctgt gcatctccgg tccgctcccg
     3481 caggagtcgg gtgaggaatt cggcggtgag atctgattcc gcgaatggca tcatcaccga
     3541 actcgttcgg ttcatgtggc gtccctttgc gaccccggcg accggggacg gcactttggc
     3601 cagaatctcc gatatcgacg gcaccggccg ccccaaatag ccgaccgcca agcagtaatc
     3661 cagcagacgg cggaggtagt ccacaccggt tgccggcggg acgaggccga gcagtgtcct
     3721 ggtgttggtg tcgtcgaatg tgcgtatccg atcgagatag caggtgtatt gggcgcccaa
     3781 gcgctcgtag tagtcgcgtt ggatcgggtc gagatcctcg acggagaact ctgtcggcgg
     3841 tacgaaggtg ccgatctcga aggacggata ttcggcgagc acgtcggtca cctcacgtag
     3901 cgacaacgtt tgggcgcccg gaagatggaa tgttcggccg aggatggatg tatcaccggt
     3961 gaggaccagt tcggctgctg ccacaacggc gtcggcgacg tagtccacgg gaaccagaga
     4021 gagggtggcg tcgtagcagc cggggaggcg ccgcagtctc ccctcgacgg tcaacttgag
     4081 gagggtatag agattcctga agtcgcggac ggcgccggtg gcggcgtccc cggcgacgat
     4141 cgcgggacgc agaatgaccg aatccaccgg tgcctgagtc accagtcgtt cggcaaggta
     4201 cttgctccgc tcgtatccgt tgctgaattc ctgtccggca tccagctcgt gctcggagat
     4261 cgttccgttg cgacgaccgc agacgtacgc ggtgctgaca tggaccagcg gcacgttcca
     4321 tcgctgcgcc aggtcgaggg cgtgctctgt tccggcgatg ttcactcgcc ggtattccgc
     4381 ttcaggcgcg ttgaaagcga tcgtggcggc ggcgtgcacg atgcacccca ccgcgtcccc
     4441 gagctcgtcg agcacctgtt cggccacacc gaaccccggt tcgcgcacgt cgg
//
LOCUS       DASRJA010000036         9525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2502406,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000036 DASRJA010000000
VERSION     DASRJA010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2502406"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VFC01_01645"
     CDS             <1..1052
                     /locus_tag="VFC01_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN78379.1"
                     /translation="QLVAVMPAQIETMKSMQQITLTMYQSQKSQQDQSKAMQENQTAM
                     GQAFDTSKNDDSFYLPPEVFDNADFKRGLKMFVSPDGKSVRFIISHEGDPATPEGIKL
                     IPQVKSAAFEAIKGTPLEGSKIYLAGTASTYKDMQEGANWDLLIAGIASLCLIFIIML
                     LLTRAVMASAVIVGTVLMSLGTSFGLSVLIWEHVLGKPLHWLVLAMSVIILLAVGSDY
                     NLLLVSRFKEEIHAGIKTGIIRSMAGTGAVVTSAGLVFAFTMMTMMVSDLVVAGQVGT
                     TIGLGLLFDTLVIRSFMTPSIAAMMGPWFWWPQIVRMRPKTVPWPKPRQHSADTPVTV
                     GSGGPTDGNLTDPIR"
     gene            complement(1092..2816)
                     /locus_tag="VFC01_01650"
     CDS             complement(1092..2816)
                     /locus_tag="VFC01_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HZN78380.1"
                     /translation="MTKSLSDPIQAEPDGRMGHRTGLPVDRKVFAVAAALAVGFVVWG
                     AISPDKMGDLTDKAMSWVTASFGWLFVLTSGAFVLFSAYLALSRYGNIKLGADDAVPE
                     FSKFSWVSMMFATGMGIGLMFYGVAEPLYHLNGPPMGMAEPGTPEAAQLAMEYTFFHW
                     GFHPWAMYAVIGLSIGYFAYRKRHGNLVSGAFRPLLGKLVDRTPGKAIDVVAIFATLF
                     GSATSLGLGALQITGGIDRVFGSGRDSKWLAVGVIAFLTACFVLSAVSGIERGVKWLS
                     NANAVAAALLALFLFVVGPTVFILGTFTESLGGYLTHLPTMSFRDGVFDNEVSGPWLS
                     AWTIFYWAWWISWTPFVGMFIARISKGRTIREFVVYVILVPSLVSFIWFSILGGAAFD
                     LQLNQGKDMAAVVDAGMEGALFETLGSYPLGTVTSALAVFLIAIFFVTGADSASIVMG
                     MLSQKGCEEPRRGLTVFWGVTTGAVAAVLLWSGGLKALQTLVILVAGPFMLVLIAMCI
                     STFKSLREEPYESTLPPRVRRAVLHAQRHDRLQHQTIALATLGCDGFDDGEAPNSDTP
                     KIVVAVDD"
     gene            2986..3165
                     /locus_tag="VFC01_01655"
     CDS             2986..3165
                     /locus_tag="VFC01_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78381.1"
                     /translation="MTRPPGPGGHDYEYDEAHDALAESVTETPAPHPVSPPPKPNLDP
                     GGDYGYDEAHDFGAT"
     gene            complement(3202..3687)
                     /locus_tag="VFC01_01660"
     CDS             complement(3202..3687)
                     /locus_tag="VFC01_01660"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN78382.1"
                     /translation="MPTPRRGDASIHIAAPPDLVYELVADVTRMGEWSPECYRCEWLD
                     AATSASVGARFRGYNRRGRYRWERTAIVQAADPGREFAFTTIDDRTGRHETNWQYTME
                     SSRSGTLLSERFEFLWCSFTNRAVEMFLPRGRQVQDGVEETLARIKHAAEANTTITPA
                     D"
     gene            complement(3722..4354)
                     /locus_tag="VFC01_01665"
     CDS             complement(3722..4354)
                     /locus_tag="VFC01_01665"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN78383.1"
                     /translation="MVETQECTERWHRYWDKKSRGYDREMQFMERMLFGDSREWACSQ
                     ASGEVLEVAVGTGLNLGAYPSGIQLTGIDLSEPMLAIARTRAAELGRDVELRQGDAHA
                     LPFADAAFDAVVCTFGLCAIPNIDTALDEMTRVLRPGGKLILVDHVASSSRIARAIQR
                     AIEVITVPMASEHFLRRPLNNLKQLPFEIERAERFKLGLVERIVARKLDH"
     gene            complement(4377..5681)
                     /locus_tag="VFC01_01670"
     CDS             complement(4377..5681)
                     /locus_tag="VFC01_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN78384.1"
                     /translation="MRSPYPPGPPWPAAVQSVLFQRHRGWLLPTMARRYGDVFALRVP
                     PYADNLVVFNRPEHIKEIFAADPTDLHAGEGNQVLGPVMGEHSLLLVDEDEHARARRL
                     LMPAFNGAALRGYRDLVAELAKAEVDRWSTATEVITLDGMNALTLEVIMRVVFGVTDG
                     HTRDQLAPRLHRMVDISPIVLLGWVIPGLCRFGPWKRFNDNQSEIDALLYAEIAQRRR
                     ATDLAERTDVLSRLLQVGSETGDAPLTDAELRDQLITLLLAGHETTAAALSWTLYELA
                     RHSGLQHDGYIAARDGDDKFFEALLKEGMRLHPVIASTARKLTHEQEIGGWKLPAGTV
                     VNTSILLAHQRSESYPDTWTYRPSRFFDKEVEPNTWLPFGGGVRRCIGAGFSLMEGTA
                     VLREVLTRYNLELPADGEPERGHVRNITNVPRRRARIVVAAR"
     gene            5760..6365
                     /locus_tag="VFC01_01675"
     CDS             5760..6365
                     /locus_tag="VFC01_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN78385.1"
                     /translation="MQDPSAATGSKDRLRRAALHCIAQRGYAATSSRDIAREAGANVA
                     SINYHFGSKEALVTEALGECFGMWNQRVETAFVAAAGLAPHDQLAAILHAAIDSFAEL
                     RSSVHACIETYAPALRSEELREQMAAGYATVRESAVELATKAMQDGGMPVPENLAAIT
                     SVLMAVCDGLMLQWIADPDAPPNAAEALDALSAIGALAAMR"
     gene            6405..7088
                     /locus_tag="VFC01_01680"
     CDS             6405..7088
                     /locus_tag="VFC01_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN78386.1"
                     /translation="MIIEAQAKRDFKDRLYGEFARIGKAIASPHRLEILEILAQGERT
                     VESIAAETGLSVANTSRHLQQLRQAQLVLARREGLFVHYRLSGPEVVGLVLALRHTAE
                     QHLAEVDRVVGDFFGDRDGFEPVTPDELTRRMTNGEVVVLDVRPEQEYAAGHIAGARS
                     MPVGDISKRIGELPRDKEYIAYCRGPYCVYADEAVAMLRANGRTAQRLTDGYPEWWLA
                     GRPVRRTNK"
     gene            7110..7610
                     /locus_tag="VFC01_01685"
     CDS             7110..7610
                     /locus_tag="VFC01_01685"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 family protein"
                     /protein_id="HZN78387.1"
                     /translation="MKERTTMPVNPTVRSRLEAQGFCGLDDATLEKLAPWMRWTYTLG
                     TRVTVIGVALMSPVVLWTLAAITSVGILLPFHPFDLLYNYGMRYLTHTGPFPNSGPQR
                     RFVFVVATAWLIATGWVFHVGADVVGIALGVPLILVGALASITNFCIPSFIYNTVTGQ
                     RPREGV"
     gene            7613..8974
                     /locus_tag="VFC01_01690"
     CDS             7613..8974
                     /locus_tag="VFC01_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005257427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZN78388.1"
                     /translation="MSGIDLVPLVDVGLGNSAYLVDLGDGRALVVDVSRDLRAVYEAA
                     NRRGVTVAFAADTHLHADFLSGAHQLGATNGTKVLASAAGDREFAHMGLHDGDEVDLG
                     GLRLRALLTPGHTHEHLAFLLLEGNREIGVFTGGSLIVGSAARTDLISDDRTDELTRA
                     QYASLRRLATLPGDVQVWPTHGAGSFCSAPPGADRTSTIARELETNPLLRAPDENTFV
                     TELLGSLGSYPPYFRRLGEINRVGPPLLDGEPTMRQLSVDAVRALMADGAVLVDARPV
                     DRFAASHVPGAVSIPFRAVFASWLGWLAPGDRPLVIVRDSDQDPVEIAWQAAKIGYDN
                     VIGELEGGMVAWTSAGHEARQIGLLQSKDLDGRRVLDIRQRSEYLNGHLPGAIHVELG
                     DIPEQADDLSDEPTVVMCGHGERAMGAASLLERAGHHDVVVLDGGPGDWVEVTGGKLE
                     TGA"
     gene            8971..>9525
                     /locus_tag="VFC01_01695"
     CDS             8971..>9525
                     /locus_tag="VFC01_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN78389.1"
                     /translation="MSTTHKPRLGLRANIAQFSLLVVVNAFVGGMVGQEQAVLPLLAE
                     HEFHLSGYTFLLTYVLAFGITKAATNYFAGTWSDRFGRKPVLLVGWLIAIPVPLLLIW
                     APSWGWVIFANVLLGINQGLTWSTTVVMKIDLVGPRQRGLAMGFNEAAGYGAVALTAV
                     LAGYLAAHYGLRPAPFLLGLSYVLI"
BASE COUNT         1765 a         3019 c         3012 g         1729 t
ORIGIN      
        1 cgcaactggt cgcggtcatg cccgcccaga tcgagacgat gaagagcatg cagcagatca
       61 cgctgacgat gtatcagagc cagaagagtc agcaggacca gagcaaggcc atgcaggaga
      121 accagaccgc gatgggtcaa gcgttcgaca cctccaagaa cgacgattcg ttctatctgc
      181 cgccggaggt attcgacaac gcagatttca agcgcggcct gaagatgttc gtctcaccag
      241 acggaaagtc cgtgcggttc atcatctctc acgagggtga tcccgcgact cctgaaggca
      301 tcaagctgat tccgcaggtc aagagcgcgg cgttcgaggc catcaagggc acgccgctgg
      361 agggctcgaa gatctatctg gcgggtaccg cgtcgaccta caaggacatg caggaaggcg
      421 ccaactggga ccttctgatc gccggaatcg cctcgctctg cttgattttc atcatcatgc
      481 tgttgctgac ccgagcggtg atggcctcgg cggtcatcgt cggtacggtg ttgatgtcgt
      541 tgggtacttc cttcgggctg tccgtgctca tctgggaaca cgtcctcggc aagccgctgc
      601 attggttggt gctggcgatg tcggtgatca tcctgttggc ggtgggatcc gactacaact
      661 tgttgttggt ctccaggttc aaagaggaaa tccatgctgg gatcaagacc ggcatcattc
      721 ggtcgatggc cggcaccggc gcggtggtca cgtctgccgg cctggtgttc gcgttcacca
      781 tgatgacgat gatggtcagc gacctggtgg tggccggtca ggtgggcacg acgatcggtc
      841 tcggcctgct gttcgacacg ctggtgatcc gatcgttcat gacgccgtcg atcgcggcga
      901 tgatgggccc ctggttctgg tggccacaga tcgtccggat gcggccgaaa accgtgccat
      961 ggccaaagcc acgacagcac tcggccgata ccccagtcac cgtcggctca ggcggaccta
     1021 ccgacggaaa cctgacggac ccaatcagat aaggccgtgg aggattccgc cggtgtccag
     1081 agatatcggt gtcagtcgtc cacggcgacg acgatcttgg gtgtatcgga gttaggcgct
     1141 tcaccgtcgt cgaacccgtc gcagccgagg gtggccaggg cgatcgtctg atgctgcaac
     1201 cggtcatggc gctgggcgtg gagcactgcc cgacgtactc gcggcggcag cgtcgactcg
     1261 tacggttcct cccgcaatga cttaaacgtc gatatgcaca tcgcgatcag taccaacatg
     1321 aacgggcctg ccaccaggat caccagggtc tgcagggcct tcagtccgcc ggaccacagc
     1381 agcaccgctg ccaccgcgcc ggtcgtcaca ccccagaaga cggtcagccc gcggcggggc
     1441 tcttcacaac ctttctggct gagcatgccc atcacgatcg aggcgctgtc ggcgccggtg
     1501 acgaagaaga tcgcgatgag aaacactgcc agcgcggagg tgaccgtacc tagtggatag
     1561 ctgccgaggg tctcgaacag ggcgccctcc attcccgcgt cgaccaccgc ggccatgtct
     1621 ttgccctggt tcagctggag gtcgaacgcg gcgccgccaa ggatcgagaa ccagatgaac
     1681 gacacgagac tggggaccag gatcacgtag accacgaact cgcgtatcgt gcggcctttc
     1741 gagatcctgg cgatgaacat gcccacgaag ggtgtccagg atatccacca ggcccagtag
     1801 aagattgtcc acgccgacag ccacggcccg gacacctcgt tgtcgaaaac gccgtcgcgg
     1861 aagctcatcg tcggcaggtg ggtgaggtag cccccgagcg actcggtgaa ggtaccgagg
     1921 atgaaaacgg ttggcccgac aacgaatagg aagagcgcga gcaaggccgc ggccaccgcg
     1981 ttggcgttgg acagccactt gacgccgcgc tcgatcccgc tcaccgcaga caggacgaag
     2041 caggcggtca ggaatgcgat caccccgacg gccagccatt tggagtcacg gccggagccg
     2101 aatactcggt cgattccgcc ggtgatctgt agcgccccca atccgagcga cgtcgcggat
     2161 ccgaacaggg tggcgaagat ggcgaccacg tcgatcgcct tgcccggggt gcggtcgacc
     2221 agtttgccca acagcggccg gaaggcaccg gacaccaggt tgccgtggcg cttgcggtag
     2281 gcgaagtacc cgatcgacag gccgatcacg gcatacatcg cccaggggtg gaagccccaa
     2341 tggaagaagg tgtactccat cgccagctgc gcggcttcgg gcgtgccggg ctcggccatc
     2401 cccatcggcg ggccgttcag gtgatacagc ggctcggcga cgccgtagaa catcaggccg
     2461 atacccatcc cagtggcgaa catcatcgat acccacgaga acttggagaa ttccggcaca
     2521 gcgtcatcgg cgcccaattt gatgttgccg tagcggctta gcgccaggta ggcggagaac
     2581 agcacgaatg cgccgctggt gaggacgaac agccagccaa atgacgccgt cacccaggac
     2641 atcgccttat cggtgaggtc gcccatcttg tccggcgaga tcgcgcccca gacgacgaag
     2701 ccgacggcga gggcggcggc cactgcgaag accttgcggt ccaccgggag gcctgttcga
     2761 tgtcccattc ggccgtcggg ctcggcctgg attgggtcgg acaagctctt ggtcattgga
     2821 gctcctcggt cgctggcagt tgttccgcta ttcacaacac agtgctcgat acgcaacgta
     2881 tccacgcata gtgatccacg acacacggcc gagtcaagac cctgttttct gttcggcgca
     2941 ccatggcgtc cgcccaccaa tcgcgtcgat aatgaggaag tgcaaatgac ccggccacct
     3001 ggcccagggg gccacgacta tgagtacgac gaagcacacg acgcgctggc cgagtcggtg
     3061 actgagactc ccgcgccgca tcccgtctct cccccgccga agccgaacct cgacccgggc
     3121 ggggactatg gctatgacga agcgcacgac ttcggcgcca cctgaaccct gcgacgaatc
     3181 ctcggccgcc acagatgcgc gtcagtcagc cggcgtgatc gtcgtgttcg cttcggccgc
     3241 atgcttgatg cgcgcgagtg tttcctcgac gccgtcctgc acctgacgtc cgcgggggag
     3301 aaacatctcg acggctctgt ttgtgaatga gcaccagaga aattcgaacc gctcgctgag
     3361 caaagtcccc gagcgcgatg actccatcgt gtattgccaa ttagtctcgt ggcggccggt
     3421 gcggtcatcg attgtggtga aagcgaactc ccgccctggg tcggcggcct gcactatggc
     3481 ggttcgttcc caccggtacc tgccgcgtcg gttgtacccc cgaaaccgcg cgcccaccga
     3541 ggcactggtt gccgcgtcga gccattcgca tcggtagcac tccggactcc attcgcccat
     3601 ccgagtcaca tcggcgacca gttcatacac caggtccggt ggcgcagcga tgtgaatgct
     3661 cgcgtcgcca cgtcgaggtg tcggcatgat cagcgtctcc acgcggtcat gtcttagcta
     3721 ctcaatgatc gagctttcgg gcgacgatgc gctcgacaag ccccagcttg aatcgttctg
     3781 cccgttcgat ttcgaatggc agctgcttca agttgttcag cggtcgccgc aagaagtgct
     3841 cgctggccat gggtactgtg atgacttcga ttgcgcgctg gatggcgcgt gcaatgcgcg
     3901 acgaactcgc cacgtgatcg acgagaatca acttgccacc ggggcgcagg acgcgggtca
     3961 tctcgtcgag cgcggtatcg atgttgggta tcgcgcatag cccgaaggta cagacgacgg
     4021 cgtcgaacgc cgcgtcggcg aagggcaacg catgcgcgtc gccctgacga agctcgacat
     4081 cacgtcccaa ctcggccgcg cgcgttctgg cgatcgccag catcggctcg ctgagatcta
     4141 taccagttag ctgaatgccc gacgggtaag cgccgagatt cagacccgta ccgacggcca
     4201 cctcgagaac ctcaccgctg gcctgcgagc aggcccactc gcgcgagtcg ccgaacagca
     4261 tccgttccat gaattgcatc tcgcggtcgt agccgcggga tttcttgtcc cagtagcgat
     4321 gccaccgttc ggtgcattct tgtgtctcaa ccatggcgaa cccttctttg tcggcgctaa
     4381 cgggcggcga cgacgatgcg ggcacgccgc cgcgggacgt tcgtgatgtt gcggacatga
     4441 ccgcgctcag gctcgccgtc ggccggcagt tcgaggttgt accgggtgag gacctcacgc
     4501 agcaccgcgg tgccttccat gagcgaaaac cccgccccaa tgcaacggcg cactccgcca
     4561 ccgaacggta gccacgtgtt gggttctact tccttgtcga agaaccggct cggccgatac
     4621 gtccacgtat cggggtagct ttcggagcgt tggtgagcca gcaggatcga cgtgttgacg
     4681 accgttcccg ccggcagctt ccacccaccg atctcctgct cgtgcgtgag tttgcgagcg
     4741 gtggaggcga taacaggatg cagccgcatg ccctctttca gcagggcctc gaagaacttg
     4801 tcgtcgccgt cgcgggcggc gatgtagccg tcgtgctgca gcccggaatg gcgggccagt
     4861 tcgtacaacg tccacgacaa cgctgcggcg gtggtttcat gtccggcgag gagcagggtg
     4921 atcagctgat cccgcaactc ggcgtcggtc agcggtgcgt ccccggtttc actgcccact
     4981 tgcaacagcc gggacagcac atcggtgcgc tcagccagat cggtcgctcg tcggcgctga
     5041 gcgatctcgg catagagcaa ggcgtcgatc tcggattggt tgtcattgaa ccgtttccac
     5101 ggaccgaagc gacacagtcc gggaatcacc caacccaaca acacgattgg actgatgtcg
     5161 accatgcggt gcagccgcgg ggcgagctga tcacgcgtgt gtccatccgt tacaccgaac
     5221 acgacgcgca tgatcacctc gagggtcagc gcattcatcc cgtccaatgt gatcacttcc
     5281 gtggcggtgc tccatcggtc gacctcagcc ttggcgagtt cggcgacgag gtcgcggtag
     5341 ccgcgcagcg cggcaccgtt gaaggccggc atgagcagtc gacgggcacg cgcatgttcg
     5401 tcctcgtcga cgagcaacag ggaatgctca cccatgacgg gcccgaggac ctggttgccc
     5461 tcaccggcat gcaggtcggt ggggtcggca gcgaatatct ccttgatgtg ttcgggccgg
     5521 ttgaacacca cgaggttgtc ggcataaggc ggaacgcgca aggcgaacac gtcgccgtag
     5581 cggcgggcca tcgtcgggag cagccagccg cggtggcgtt ggaacagcac gctctgaacc
     5641 gcagccggcc acggcggacc gggcggatac ggagatcgca tgagcttctc cctcgacacg
     5701 tcggcgttcg ataaacgctt gtttcattca aacgtttgtt tatcacctat gatggctcca
     5761 tgcaagatcc gtctgcagct accggcagca aggaccggct tcgtcgcgct gcgctgcatt
     5821 gcattgcaca gcgaggctat gccgccacgt cgtcgcgtga catcgcccgg gaggccggag
     5881 ccaacgtcgc ctcgatcaac tatcacttcg gcagcaagga agccctggtc accgaggcgc
     5941 tgggcgagtg cttcgggatg tggaaccaac gcgtcgagac cgcgttcgtc gcggccgccg
     6001 gccttgcgcc ccacgaccaa ctcgcggcca tcttgcatgc tgccatcgat tccttcgccg
     6061 aactgcggtc atccgtacac gcgtgcattg aaacgtacgc accggcactg cgatccgagg
     6121 agctgcggga acagatggcg gccggatacg cgacggtgcg cgagtccgcg gtcgaactcg
     6181 cgacgaaggc catgcaggac ggcgggatgc cggtcccaga aaacctcgcc gcgatcacgt
     6241 ccgtgctgat ggcggtgtgc gacggcctga tgctgcagtg gattgcagac ccggacgctc
     6301 cgccgaacgc cgcagaggcg ctcgacgccc tcagcgcaat cggcgcattg gcggccatgc
     6361 gctaaggggt tcccaccacg ctattcaagg attacattga ataggtgatt atcgaggctc
     6421 aggccaaacg cgacttcaag gaccgcctct atggcgagtt cgcccgcatc ggcaaggcca
     6481 tcgctagccc acatcgcttg gagatcctcg agatcctggc gcagggcgag cgcacggtgg
     6541 agtccatcgc cgccgagacc ggcctgtcag tggcgaacac ctcccggcac ctgcagcaac
     6601 tacggcaggc gcagctcgtg ctcgcccggc gcgaaggact attcgtccac taccggctca
     6661 gcgggcccga agtcgtcggc ctcgtgctgg cgctgcgaca taccgccgag caacacctcg
     6721 ccgaggtgga ccgcgtagtg ggtgactttt tcggtgatcg tgacggcttc gagcccgtca
     6781 cgccggacga gctgacgcgc cgcatgacca acggcgaagt cgttgtgctg gatgtgcggc
     6841 ccgagcagga gtacgcggcg ggacatatcg ccggtgcgcg gtccatgccc gtgggggaca
     6901 tctcgaagcg aatcggcgaa ctcccccgcg acaaggagta catcgcgtac tgccgcggac
     6961 cttattgcgt gtatgccgac gaagccgtgg cgatgctgcg ggcgaatgga cgtacggcgc
     7021 aacggcttac cgacggatac ccggagtggt ggctcgcggg gcggccggtg cgcaggacga
     7081 acaaatagtc accacaccgg ggagcaagca tgaaagaacg tacgaccatg cctgtgaatc
     7141 cgaccgtccg cagtcgcctt gaagctcaag gcttttgcgg actcgacgac gccacgcttg
     7201 agaagctggc gccatggatg cgctggacat acacgctcgg gacacgggtc acggtgatcg
     7261 gcgtggcgct gatgtcaccc gtggtgctgt ggacactcgc ggccatcacc tccgtgggca
     7321 ttcttcttcc gttccacccg ttcgatcttc tctacaacta cgggatgcga tacctgaccc
     7381 atacgggacc cttcccgaac tctgggcccc aacgacgatt cgttttcgtc gtcgcgaccg
     7441 cttggctgat cgcaaccggc tgggtgttcc acgttggcgc cgacgtcgtg ggaatcgctc
     7501 tcggtgtccc gctgattctg gtcggtgcgc tggccagcat caccaacttc tgcatcccgt
     7561 cgttcatcta caacaccgtg accggccagc ggcccaggga gggagtttga ggatgtccgg
     7621 tattgacctg gtgccgttgg tcgacgtggg cctcggcaac tctgcctatc tcgtcgatct
     7681 cggcgatgga cgcgcgctcg tcgtcgacgt gagtcgcgac ctgcgcgccg tgtacgaggc
     7741 agccaaccgt cgcggtgtga cggtggcctt cgccgccgac acccatctgc acgccgactt
     7801 cctgtccggt gcacaccaac tcggggcaac aaacggaact aaagtactgg cgtcggcggc
     7861 cggcgaccgc gagttcgcac acatggggct tcatgatggc gacgaagtcg acctcggcgg
     7921 attgcggctg cgcgcgttgc tgacacctgg ccacacgcat gagcacctgg ccttcctgct
     7981 gctcgagggg aaccgcgaga ttggtgtgtt caccggtggg tcgctaatag tgggttcggc
     8041 ggcccgcacc gacctcatct cagatgaccg gactgatgaa ctcacccgag cgcaatacgc
     8101 gtcactgcgc cggttggcca cgctgcccgg cgacgtacag gtgtggccga cccatggtgc
     8161 cggatcgttc tgctcggcac cccccggggc agatcgcacc agtaccatcg cccgcgagct
     8221 ggaaaccaac ccactgctga gggcacccga cgaaaacacg ttcgtcacag agcttctcgg
     8281 atcactgggc agctatccgc catatttccg gcggcttggc gagatcaacc gcgtcggccc
     8341 cccactcctc gacggcgaac ccactatgcg ccagctatct gtcgacgcag tgcgcgctct
     8401 gatggcagac ggtgcggttc tcgtcgatgc tcgacccgtc gaccgcttcg ccgccagcca
     8461 tgtaccgggc gccgtctcca tcccgttccg tgcggtgttc gcgagctggc ttggctggct
     8521 ggcgcctggc gatcggccat tggtgattgt gcgcgacagc gaccaggacc ctgtcgaaat
     8581 tgcctggcag gctgcgaaaa tcggctacga caacgtgatc ggagaactcg aggggggcat
     8641 ggtcgcttgg acgtcggccg gccatgaggc ccgacaaatc ggtctccttc aatcgaaaga
     8701 tctcgacggc cgccgggtat tggacattcg tcagcgttcg gaatacctga acgggcatct
     8761 gccaggcgcg attcatgtcg aactcggtga cattcccgaa caggcagacg acctctccga
     8821 cgagccgact gtggtcatgt gcggccacgg tgagcgggcg atgggtgcgg cgagcctgct
     8881 cgagcgcgcg gggcaccatg acgtcgtcgt actggatggc ggaccagggg actgggtcga
     8941 ggtcaccggc ggcaagctcg agacgggcgc atgagcacaa cccacaagcc ccgacttggg
     9001 ttgcgcgcca acatcgccca attctctctt ctggttgttg tcaacgcgtt tgtgggtgga
     9061 atggtcggcc aagaacaggc cgtgcttcca ctgttggccg aacacgagtt ccacctcagc
     9121 ggctacacgt ttctgctgac ctatgtgttg gcgttcggga tcaccaaggc cgccaccaac
     9181 tacttcgccg gcacctggtc agatcggttc ggacgcaagc cagtgttgct ggtcgggtgg
     9241 ctgatcgcga ttccggttcc gctgctgctg atttgggcgc cctcctgggg atgggtgatc
     9301 ttcgccaatg tcctgctagg catcaaccag ggcctgacgt ggtcgacaac ggttgtgatg
     9361 aagatcgacc tcgtcgggcc gcggcaacgc ggactggcga tgggtttcaa cgaagccgcc
     9421 ggctacggcg ccgtggcgtt gacagcagtc ctggccggct acctcgccgc gcactatggt
     9481 ctgcgacccg cgccgttcct gctcggcctg tcctatgtgc tgatc
//
LOCUS       DASRJA010000037         4552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3348087,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000037 DASRJA010000000
VERSION     DASRJA010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3348087"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VFC01_01700"
     CDS             complement(<1..450)
                     /locus_tag="VFC01_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78390.1"
                     /translation="MTINEQSRVTTSRDHGTLQALAGTRALVDRLNAGEPYTVAFGGQ
                     GGSWLDNLEELVSSAGIESELSEIVGEAALLLEPVARELVVVRPIGFEPLQWVRALAA
                     DEPLPPAKQLISTAISAPGILLAQMAAIRAVIRQGLDLHGAPPVAMAG"
     gene            complement(947..1681)
                     /locus_tag="VFC01_01705"
     CDS             complement(947..1681)
                     /locus_tag="VFC01_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HZN78391.1"
                     /translation="MAISRRDVLKYAAAAPAALGLGAGLQAVSSALAAPRATAAPLGI
                     LLDYAAGIIKAGDLRASGALGAIRYVSDRRPGGAWMLGKPIQLPEARDLYQNGLKIVS
                     CYQYGKQDTADWLGGQNAGVAHAKRGWQLHVGAGGSYGAPIYASIDDNPSYEQYKSQV
                     APYLRGWEAVLGHQRVGVYANARTIDWALQDGLGSYFWQHNWGSPQGFTHPAAHLHQV
                     EIDKRNVGGIGVDLNHILKPRFGQWD"
     gene            1861..2943
                     /locus_tag="VFC01_01710"
     CDS             1861..2943
                     /locus_tag="VFC01_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN78392.1"
                     /translation="MPSGTVRSQPGANVTAAVDDPQIELIGVRKVFGEIVAVEGADLT
                     VDDGELFAILGPSGSGKTTVLRMIAGFEQPTAGVIKLGGTVVTALPARHRDVNTVFQE
                     YALFPHMTVAQNVEYGLKVKGVPKAERRRRTADALDMVRLTGQAPRRPAQLSGGQRQR
                     VALARALIGRPRVLLLDEPLGALDLKLREQMQIELKAIQREVGITFVIVTHDQDEALT
                     LCDRLAVFNDGRIEQVGNARDVYENPANRFVADFVGTSNVLDGAAAEALMGRPGMFVI
                     RPERIGVFRPGTDPVPGVHTVEATVSEIIYAGPTTRIAATAAPDVTLTATVLTASTWL
                     PPDLHHGTQITLAWPEKAIHQLKVEE"
     gene            2946..4136
                     /locus_tag="VFC01_01715"
     CDS             2946..4136
                     /locus_tag="VFC01_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN78393.1"
                     /translation="MPSSAVRIGLVLTACAALVVACSSSDKSGGNGQTPPKLEPLSAV
                     GNGEGQLNLIAWAGYAEDGSNDKTIDWVTPFEQKTGCQVNVKIGNTSDEMVQLMRSGQ
                     YDGVSASGDATLRLIYAGDVAPVNTSLVPNYATISSFLKDGAWNSVDGQMYGIPHGWG
                     ANLLMYNIDVVRDAPNSWSAVFDDAGKYKGKVTAYDSPIYIADAALYLSKTKPDLGIK
                     DPYSLTSEQLDAAVDLLKKQRENVSEYWSDYTKEVQAFESGTSVIGTTWQVIANTIGT
                     DNKVQVNTVLPKEGSTGWSDTWMVSAKAAHPNCMYKWMDWITSPEVNAQVAEYWGEAP
                     AQTKACDHISQKDFCDIYHATDEKFASELHYWTTPQKQCVDGSGDNCTAYQDWVDKWQ
                     QIKG"
     gene            4115..>4552
                     /locus_tag="VFC01_01720"
     CDS             4115..>4552
                     /locus_tag="VFC01_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN78394.1"
                     /translation="MAADQGLTQRRTTLPIAVGRRISLALLLVPPLAWLVVGYLGSLA
                     VLLVSAFWSTNAFTGAVVRTVTTDNIVRVLTDSVFRIATVRTVSIALVVTVLCVALAV
                     PLAFYMAKVASPRVRLALVVAVTTPLWASYLVKAYAWRMLFSPE"
BASE COUNT          834 a         1472 c         1459 g          787 t
ORIGIN      
        1 tccggccatc gccaccggcg gggcgccgtg gagatcgagg ccctggcgga tgacggcacg
       61 gatcgcggcc atctgggcga gcaagatgcc gggagcggag atggcggtgc tgatcagctg
      121 cttggcgggg ggcaacggct catcggcagc cagagcgcga acccattgca gcggctcgaa
      181 gccgatcggc cgcactacga cgagttcacg ggccaccggc tcgaggagca gagcggcctc
      241 accaacgatc tcgctcagct cggattcgat gcccgccgaa ctgaccagct cttccaggtt
      301 gtccagccag gagccaccct ggccgccaaa cgcgaccgta tacggctctc cggcgttcaa
      361 acgatcgacg agggcgcgtg tacctgccaa tgcttgcaaa gtgccgtggt cgcggctggt
      421 ggtcaccctg ctctgttcgt tgatcgtcac gtcgtgttat ctccctcggt actgtctcgc
      481 gcgtatggct cgtctgtgct gtcgtctctg gggctgtgca ggcccgcgct gcgggggcca
      541 tgcgattcgg cgtcgaatcc cgactggttc atgcgcgtcg ggcgcacggg ccaccggtgc
      601 ggccactcca gtcgtactaa gagtgtcata agagacggca ccattttcgc gccccaaatg
      661 gttactggcg agttctacgc atgggtaacc gtgcattcga taacaccggc ctgcacaacc
      721 ctcgaacgta tgcgggacaa acgtcctgca tgcaggtggt tacggtcgag taggcacaac
      781 agtgcagatc aaagcagcat tgttatgtaa tcgttatgta aaaattccgg cctgcctcac
      841 cagccgcatt gaggcgcccc gagtcgacaa ctcggcgaga tttgtcttct cgaagccact
      901 agctactgga aatagtttgc tgtccctact tactgatcgg taaggtttag tcccactgcc
      961 cgaaccgggg cttgagaatg tggttgaggt cgaccccgat gccccccaca ttgcgcttgt
     1021 cgatctcgac ctgatgcagg tgtgcggcgg gatgcgtgaa cccctgcggc gaaccccagt
     1081 tgtgctgcca gaagtaggaa cccagcccgt cctgcagcgc ccagtcgatc gtcctggcgt
     1141 tggcgtacac cccgactcgc tgatggccga gcaccgcctc ccagccacgt agatacggcg
     1201 ccacctggga cttgtactgc tcgtagctgg gattgtcatc gatcgacgcg tagatcggcg
     1261 cgccgtacga cccgcccgct ccgacgtgta gctgccaacc ccgcttggca tgggcgacgc
     1321 ccgcgttctg cccgcccagc cagtccgcgg tgtcctgctt gccgtactga tagcacgaga
     1381 cgatcttcag cccgttctgg tagaggtcgc gggcctcggg cagctggatc ggcttgccga
     1441 gcatccaggc accgcccggc cgccgatcag agacgtacct gatcgcgccc agcgcaccgg
     1501 atgctcgcag atcgcccgcc ttgatgatgc ccgcggcata gtcgagcagg atcccgagcg
     1561 gagccgccgt cgcccggggg gcggccagcg ccgacgacac cgcctgcagg ccggcgccga
     1621 gaccaagcgc ggcgggcgcg gcggcggcgt atttgagcac gtcgcgccgg gatatagcca
     1681 cgtgccacag ggtacgacag gcgcatcaat tttcacgtca acgccactgg tcacatctgt
     1741 atcaaaaatt cgcaaacagg ctggggcggg cgttgatacg aaaccagtgg tcgatgggtc
     1801 ccaagatcac gaaatccggg atgaagctgc gccaacaccg cggaattcct acgatgagcc
     1861 atgccctcgg ggacggtccg ctcgcagccc ggtgcgaatg tcactgccgc cgttgacgat
     1921 ccgcagatcg agctgatcgg tgtccgcaag gtcttcggcg agatcgtcgc ggtcgagggc
     1981 gccgacctga cagtggacga cggcgaactg ttcgcgatcc tcggcccatc tggctcggga
     2041 aagacgacgg tgttgcggat gatcgcgggg ttcgagcagc ccaccgccgg cgtgatcaaa
     2101 ctcggcggta ccgtcgtgac cgcgctgccg gcccgccacc gcgacgtcaa caccgtcttc
     2161 caggagtacg ccctcttccc gcacatgacc gtggcgcaga acgtcgaata cgggctgaag
     2221 gtcaagggcg tgcccaaggc cgaacgcaga cgccgcaccg ccgacgcgct cgacatggtt
     2281 cgcctgaccg gccaggcgcc gcgacgcccc gcacaactgt cgggaggcca gcgtcaacgc
     2341 gtggcgctcg cccgggcgct gatcggccgg ccgcgggtgt tgctgctcga cgagcctctt
     2401 ggcgcactgg atctcaagct gcgcgagcag atgcagatcg aactgaaggc gatccagcgc
     2461 gaggtcggca tcacgttcgt catcgtcacg cacgaccagg atgaggcgct gacgctgtgc
     2521 gatcgactgg cggtcttcaa cgacgggcgc atcgagcaag tcggcaacgc ccgcgacgtc
     2581 tacgagaatc cggccaaccg cttcgtcgct gatttcgtcg gcacctccaa cgtgctcgac
     2641 ggcgccgccg ccgaagcgct gatgggccgg ccggggatgt tcgtcatccg gcccgaacgc
     2701 atcggggtgt tccggccggg caccgatccg gtgcccggcg tgcacacggt cgaggcgacg
     2761 gtgtccgaga tcatctacgc cggccccacc acgcgtatcg ccgcgaccgc tgcacccgac
     2821 gtcacgctca ccgcgaccgt cctgaccgcc agcacatggc tgccgccgga cctgcaccac
     2881 ggaacacaga tcacgttggc ctggcccgaa aaggccatcc accaattgaa agtcgaggag
     2941 tagccatgcc atccagcgcc gtgcgaatcg gtcttgtgct caccgcctgc gccgcattgg
     3001 tggtcgcctg ttcgagttcg gacaagtccg gcggcaacgg tcaaacgcca ccgaaactcg
     3061 aaccactgtc ggccgtcggc aacggcgaag gtcagctgaa tctcatcgcg tgggcggggt
     3121 atgccgagga cggctcgaat gacaagacca tcgactgggt cacgccgttc gagcagaaga
     3181 ccgggtgcca ggtcaacgtg aagatcggca acacctccga tgagatggtg cagctgatgc
     3241 gcagcggtca gtacgacggt gtttcggcct cgggcgacgc cacgttgcgg ctgatctatg
     3301 cgggtgacgt ggcgccggtg aacacctcgc tggtgcccaa ctacgcgacg atttcgtcgt
     3361 tcctcaagga cggggcgtgg aattcggtgg acgggcagat gtacgggatc ccgcacggct
     3421 ggggcgccaa cctgttgatg tacaacatcg acgtggtgcg tgatgcgccg aactcctgga
     3481 gcgcggtgtt cgacgacgcg ggcaagtaca agggcaaggt caccgcctac gactccccca
     3541 tctacatcgc cgacgccgcg ctgtatctgt ccaagaccaa accggatctc ggcatcaagg
     3601 acccgtactc gctgacgtcc gaacaactcg atgccgctgt cgatttgctc aagaagcagc
     3661 gtgagaacgt cagcgagtac tggtcggact acaccaaaga ggtgcaggcg ttcgaatccg
     3721 gaacatcggt catcggcacc acctggcagg tgatcgcgaa caccatcgga accgataaca
     3781 aggttcaggt caacaccgtg ctgcccaagg aaggttccac cggatggtcg gacacctgga
     3841 tggtgtcggc gaaggcggcg catcccaact gcatgtacaa gtggatggac tggatcacct
     3901 cgccggaggt caatgcccaa gtcgccgagt actggggtga ggcgcccgcg cagaccaagg
     3961 cttgcgacca catcagccag aaggacttct gcgacatcta tcacgcgacc gacgagaagt
     4021 tcgcctccga gctccactac tggaccacac cgcagaagca gtgcgtcgac ggcagcggcg
     4081 acaactgcac ggcgtaccag gattgggtcg ataagtggca gcagatcaag ggctgaccca
     4141 gcgccgcacc acgcttccaa tcgcggtcgg gcgccgcatc agcctggcgc tgttgctcgt
     4201 cccgccgctg gcttggctgg tggtggggta cctgggttcg cttgcggtgc tgctggtttc
     4261 ggcgttctgg agcaccaacg cgttcaccgg cgcggtggtg cgcaccgtca ccaccgacaa
     4321 catcgtccgg gtattgacgg actcggtgtt ccggatcgcg acggtacgca ccgtgtccat
     4381 cgccttggtg gtgaccgtct tgtgcgtggc gcttgccgtg ccactggcgt tttacatggc
     4441 caaggttgcc tcgccgcggg tgcggctggc cttggtggtg gcggtgacca cgccgctgtg
     4501 ggcgagctac ctcgtcaagg cctacgcctg gcggatgctg ttctcgcccg ag
//
LOCUS       DASRJA010000038         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1644894,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000038 DASRJA010000000
VERSION     DASRJA010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644894"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFC01_01725"
     CDS             <1..195
                     /locus_tag="VFC01_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZN78395.1"
                     /translation="HRSAARGAPPARWWPDQWEIATQLVLSVRTVDRHVAEILAKLGV
                     TSRRQAAARADALGIASART"
     gene            349..1368
                     /locus_tag="VFC01_01730"
     CDS             349..1368
                     /locus_tag="VFC01_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZN78396.1"
                     /translation="MIGNTTSPAATPRRVVIAAGGVAGLSLARALKNAGHSPLVIERS
                     SEWPATGTADYLPANAVRALDRLGIADDVAAAAHPISRQRVTGTRGRDLVDLPVSSIW
                     GEGASCVAMRRSALHKLLQAATCDVPARLGTTITARLRGHMIRLSDGSVEPYDVLVGA
                     DGTDSVVRTAGLSGTEPRPTGRWCWQFIAEGWDGDDDTWHARLAAGRSLVTMPLGDGA
                     VYCYADLTAGNGRPPGDWRSYFNGFGTQIDAAHAMSPSIAQGVALAVEDALVLADTLT
                     SLPVEEALSAYQERRAPRIAWVRAQAHRRDNTRGLTPAVRDKVLRLTRRRVALAGQRE
                     LLAMP"
     gene            1491..2447
                     /locus_tag="VFC01_01735"
     CDS             1491..2447
                     /locus_tag="VFC01_01735"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HZN78397.1"
                     /translation="MSNAIIKQQGDSPIVADNIWEFRLGAGSFILGALAGTLVTAAVG
                     RLTAKGDAFPEPPTPRGWPINFAHRGGAKVVPENTIEGFREGFAMGGGVVECDVHASA
                     EGTIVVIHDAVVDRTTDGTGPVAEKTVSELQRLDAGYRFSTDGGLTFPWRAKGVKIPT
                     LEALYEAFPDAPFNIEIKGHRSGIEETVFRQIEAAEAMQRTLVVSDSRGTISRFRRVS
                     QGKVATASSTVELLIYRILHVLHLGALYDPPFQALQPPEKYKDVIPVVTRRFVRKAHD
                     RGLRVDVWTIDDEPAMRRLLSFGVDGIMTDRPDLLARVLNSQ"
BASE COUNT          434 a          801 c          794 g          446 t
ORIGIN      
        1 caccgatcgg cagctcgagg tgctccgcct gctcgctggt ggcctgacca atgggagatc
       61 gcgacgcaac tcgtgctgtc ggtccgtacc gtcgaccgcc acgtcgccga gatcctggcc
      121 aagctgggcg tcacatcccg gcgccaggct gctgctcgtg ctgacgcgct tgggattgcg
      181 tcggcgcgca cctaggtagc cctgtcggtc gactacccat tcccgcgaac ggggtcgacc
      241 gccgacctgg gtagtccgtt gcactgattt cggtaacgcc tgccgatgct cttgtatgcc
      301 tgcagctcgt agaactgagc aaggatcttt gaaccatgag gagacatcat gatcggcaac
      361 acaacctctc cggcagctac accgcgacgc gtcgtcattg ccgccggtgg cgtcgccgga
      421 ctgagtctgg cccgcgccct caagaacgct ggccactccc ccctggtgat cgaacgcagc
      481 tctgaatggc ccgccacagg aactgcggat tacctaccag ccaacgcggt acgggcgctg
      541 gatcgccttg gcatcgcaga cgatgtcgcc gcggctgctc acccaatcag ccgccagcgg
      601 gtcaccggga ctcgcgggcg cgatctagtt gatcttccag tgtcgagcat ctggggcgag
      661 ggggcgagct gcgtcgctat gcgccgcagc gcactgcaca agttgctgca ggcggccact
      721 tgcgatgttc ccgcacgcct cggaactacg atcaccgcac gtctgcgtgg tcacatgatc
      781 aggctcagcg acggctcagt cgagccctac gacgtactcg tcggcgccga cggcaccgac
      841 tcagtggtgc gcaccgccgg tttgagtggc accgagccga ggcctaccgg tcgctggtgc
      901 tggcagttca tcgccgaagg ctgggatggc gacgacgaca cttggcacgc tcgactggcc
      961 gctggtcgca gcctggtcac catgccgctg ggcgacggag ccgtctattg ctatgccgac
     1021 ctcactgccg gaaacggccg accgcctggt gactggcgca gctacttcaa cggtttcggg
     1081 acgcagatcg acgccgccca tgccatgtca ccgtccatcg cccaaggtgt ggcactggcg
     1141 gtggaggacg cgctggtgct ggcggatacg ctgaccagct tgccggtcga ggaggcgcta
     1201 tctgcgtacc aggaacggcg ggctccgcgc attgcctggg ttcgtgcgca ggctcatcgc
     1261 cgcgataaca ctcgaggact gaccccggca gttcgggaca aggtattgcg gctgaccaga
     1321 cgccgggtgg cacttgccgg ccagcgagag ctgctcgcca tgccgtgacg ggcgtgccgg
     1381 atcgcgcgcg cgaatgacga cagtaagggt gcgtatcggt cggcctgcag ctgcctctac
     1441 gttggaccac accccatggt tctcgggaac tccgcgcgaa agggtccagc atgagcaatg
     1501 cgatcatcaa gcagcaggga gattcgccca tcgtcgccga caacatctgg gaatttcggt
     1561 tgggagcggg ctccttcatt ctgggtgcgt tggcgggcac gttggtgact gctgccgtcg
     1621 ggcggctaac ggcgaagggt gatgcgttcc ccgagccgcc gactccgcgc ggctggccga
     1681 tcaacttcgc ccaccgcggc ggggcgaagg tcgtgccgga gaacacgatc gagggattcc
     1741 gcgaaggatt cgccatgggt ggcggagtcg ttgaatgcga cgttcacgcc tccgccgaag
     1801 gcaccatcgt ggtcattcat gacgccgtcg ttgaccggac gacagatggc accggacccg
     1861 tagccgagaa gacagtctcg gaattgcagc gtcttgacgc gggctaccga ttcagcaccg
     1921 acggcggcct gacgttcccg tggcgagcca agggcgtcaa gatccccacg ctcgaagcct
     1981 tgtatgaggc gtttccggac gcgcccttca atatcgaaat caagggtcat cgttccggta
     2041 tcgaagagac cgtgttccgc cagatcgaag ccgccgaagc gatgcaacgc accttggttg
     2101 tttcagacag tcggggcacg atttcgcgtt tccgcagagt gagccaaggc aaggtcgcca
     2161 cggcttcgtc caccgttgag ctgttgatct atcggatcct ccacgtgctg caccttggcg
     2221 ccctttacga cccgccattc caggcgctgc agccgcccga gaagtacaag gacgtcatcc
     2281 ccgttgtcac tcgccgattc gtacgcaagg cccacgaccg tggacttcgg gtcgacgtct
     2341 ggacgatcga cgacgaaccg gccatgcgcc ggctgctgag cttcggcgtc gacgggatca
     2401 tgactgaccg gcccgacctg ttagctcgcg tactgaacag tcaatgagct cgctagcggc
     2461 tggccagatt caagc
//
LOCUS       DASRJA010000039        20396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_242869,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000039 DASRJA010000000
VERSION     DASRJA010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20396
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242869"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VFC01_01740"
     CDS             <1..1119
                     /locus_tag="VFC01_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZN78398.1"
                     /translation="ARLGQSITAEQQAAGVVPDSPAALVTLAALHGGDPVRARSVIGR
                     AVRAVPEEEHFVSHRYRLLLGWVRMQDGQLSAAAADVASTADATLHRRDALWAAALQT
                     AIARRSGDSGAVQKHWYAAMEVLAEYSMDLFALLPLGELWVSAARMRQVDRLRHTLTE
                     SFALLESLGNPLLWSVPLHWAGVHAGILANSPEAVAPHGQALTAAAAHSPFAKALATA
                     GRTWLRVLANHVDIDEVTTAARGLSQFGLTWDATRLASQAALQTPDGRVSSAMLQLAR
                     DLKQTAAVDEAPGLEPASTVTERTGPSRPASSRLSDREREVAELLLLGIPYRDIGSQL
                     FISAKTVEHHVARIRRRLGAESRSEMLSMLRAMLAPQG"
     gene            1227..2864
                     /locus_tag="VFC01_01745"
     CDS             1227..2864
                     /locus_tag="VFC01_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZN78399.1"
                     /translation="MNSPGNHGRSLGLSVGATNLAGIRDGQSAVIRPAELTLHGQRLT
                     GFVDRVGDPVPLIAPDGSKHRSEELLADALGAMAHAVTDGMPVSDVAVTVPAHWRASV
                     VDTLRRNLRGRLPVVSDATAALTALRANPGLPTRGVIVLCDFGGSGTSITLANAARDF
                     APFGETVRLPDFSGDQIDQALLTHVVGGLAEIDPSGTAMVGSLVRLRDECRRAKERLS
                     AETATAVVAELPGQRADIRVTRTELEDLMAAPLSEFLAALDETLERYGVPAAHVSAVA
                     TVGGGARIPLITQRLSEHLRAPVVTTPHPQLTAAEGAALIAHRARVVDTATTLTPTVP
                     AAAAATAAGDATVSLVAHEQALAWSEDDASEDVLPYNDVTDSRPEVHFRHEEWQDDEP
                     PRRGPLVLFGLAAVAATIAAAVFGFTLLSDATTTPVDAAVTATSTVPAPAASAPLPAP
                     PAPQAPQQVTTVVVQKPAQRSYPQAPVAQQPPAPVTTEPTTTTPTTTTPTTTTTTPTT
                     TTTTTTTPPPPPPIDPGPGGGTGTSTPPIDPATGPGA"
     gene            2992..5973
                     /locus_tag="VFC01_01750"
     CDS             2992..5973
                     /locus_tag="VFC01_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HZN78400.1"
                     /translation="MDTQMLIRLVVGLGLTALVLVFAAKRVLWLTTLIRSGQKTSVEN
                     NRKDHLGKRITTQVEEVFGQTRLLKWSIPGIAHFFTMWGFFILASVYLEAYGLLFVHD
                     FHIPFIGRWDGLGFLQDFFAVAVLLGIATFAVIRIVREPKKHGRDSRFYGSHTGGAWL
                     ILFMIFNVIWTYALVRGSAVNTGNLPYGNGAFFSQLMGWVLNPLGHTANEWIETVALL
                     LHIGVMLVFLLIVLHSKHLHIGLAPVNVTFKRLPDGLGPLLPVESKGEAIDFEDPAED
                     AVLGRGKIEDFTWKGYLDFTTCTECGRCQSQCPAWNTGKPLSPKLVIMNLRDHLFAKA
                     PYILGDKETPLENTPEGGLGEELGGEKHDEEHHVPESGFERVMGSGPAQATRPLVGTA
                     EQLGVIDPDVLWSCTTCGACVEQCPVDIEHIDHIVDMRRYQVMMESEFPSELGVLFKN
                     LETKGNPWGQNAKDRTNWIDEVDFDVPVYGKDVDSFEGYEYLFWVGCAGAYEDRAKKT
                     TKAVAELLAAAGVKYLVLGEGETCNGDSARRSGNEFLFQQLAAQNVETLNDLFEGVER
                     VDRKVVVTCPHCFNTLGREYPQVGGNYTVLHHTQLLNRLIRDKKLVPVSPPDGGKDIT
                     YHDPCYLGRHNKVYEAPRELIGASGAKLTEMPRHADRGLCCGAGGARMWMEEHIGKRV
                     NHERVEEALDTGASAIATGCPFCRVMITDGVDDVAAANDVPKAEVLDVAQLLLGSLDK
                     TGVTLPEKGTAAKEAEERAAKAAPKAEAATAVAEPEAKPEPAAAAPATQAESPAQPAG
                     TKPVTGLGIAGGAKRPGAKKTAASTAEAPAAPAAPAKGLGIAAGAKRPGAKKAAPAAP
                     AAAATETSAPAAPAKPEPEVKGLGIAAGARRPGAKKTAAAPAEPASTATEPASSATEP
                     ASTATEPPVKGLGIAPGARRPGAKKAPAAPKPVVPQPEPASASESAAAPEPSADDNGE
                     TPAPPVKGLGIAKGARPPGKR"
     gene            complement(5995..6972)
                     /locus_tag="VFC01_01755"
     CDS             complement(5995..6972)
                     /locus_tag="VFC01_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78401.1"
                     /translation="MSTQQHTADVQSRDFFLDTYRYLRGGMAVMIVMLGASVIGERLS
                     ASCWQGSISAYYYTSAHAIFVAALCAIGAQLIVYKGSTDTEDNLLNLAGILAFVVALV
                     PTRLPERLCGETPPAVTSGLSITNNVWAVVIAMAGAQVVAWWLYWRAGGVGHRSLLGE
                     ITVWALRLVIAVGLVGFIFFRDLFYDHAHGVAAAVMFIAIIITVVITAFLVSKQQSPH
                     KGAYQRAYQIVAALMGVTLLLVIAVHLAWGWNHWVIALESALILEFAAYWIIQTIELW
                     KAPTRLELIPDEMQPQLAQRREKVGPTGLVEDIREMTQYPKDERLMRAL"
     gene            7101..8375
                     /locus_tag="VFC01_01760"
     CDS             7101..8375
                     /locus_tag="VFC01_01760"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZN78402.1"
                     /translation="MGGVTTHHVPWQTTGHHPKQRTFTQSSKLQDVLYEIRGPVHEHA
                     SRLESEGHRILKLNIGNPAPFGFEAPDVIMRDIISALPYAQGYSDSKGIMPARRAVFT
                     RYELVEGFPKFDVDDVYLGNGASELIQMTLQALLDNGDQVLIPAPDYPLWTACTSLAG
                     GTPVHYLCDETQGWMPDIADLESKITDRTKAIVVINPNNPTGAVYTREILTQIADLAR
                     KHELLLLSDEIYDKILYDDAKHIATASVAPDVLTLTFNGLSKAYRVAGYRSGWLVITG
                     PKEHASSFIEGISLLANMRLCPNVPAQHAIQVALGGHQSIEDLVLPGGRLLEQRDVAW
                     EKLNEIPGVSCVKPEGALYTFPRLDPEVYDIQDDEQLVLDLLLQEKILVTQGTGFNWP
                     TPDHLRIVTLPWSRDLANAIERLGNFLSSYRP"
     gene            complement(8376..9089)
                     /locus_tag="VFC01_01765"
     CDS             complement(8376..9089)
                     /locus_tag="VFC01_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN78403.1"
                     /translation="MSRDIETQVADDGVATLTLARPQRRNALSIKLRNEITQQLDDWA
                     ADGAVRAVVLTGAGPTFCAGFDLNEFGQADLARQIKDSSRRYHLAVWHFPKPLIAAVN
                     GPAMGGGMDLGVLCDCRIASSAAAFGHPEIKFGAPPLFTPLQWIVGLGVARELCLTGR
                     RIDAAEALRIGLVTRVTEPGDLLDGALAMARSIIEAPQAALETTKRYLIGSASATFEE
                     AFAVEHDNVFDDFLLGRIG"
     gene            complement(9143..9883)
                     /locus_tag="VFC01_01770"
     CDS             complement(9143..9883)
                     /locus_tag="VFC01_01770"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZN78404.1"
                     /translation="MLTDAGTVLAEHADRVLAAERAALDAVLNVRDHVAAPVLLGTFG
                     STAAALLPAVVARAHAAYPQMRLRSIELDVDDALDAVTRGRVDAAFGLDYPSVPLPRL
                     PGVDIITLREERFGLAVSPGAYGMRTESRIDLRDAADWDWILPPSDTHYGAAMRTVCR
                     QHGFEPATSHEIIDTAVTLALAAKGLGIAPVTDMMIGLNSSVPIIRIALKQEIVRKIV
                     LIRPAGADARPTVRAVTEIVQHVISDRG"
     gene            complement(9957..10055)
                     /locus_tag="VFC01_01775"
                     /pseudo
     CDS             complement(9957..10055)
                     /locus_tag="VFC01_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10144..11709
                     /locus_tag="VFC01_01780"
     CDS             10144..11709
                     /locus_tag="VFC01_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN78405.1"
                     /translation="MSTSISTITETVCLPSTDELRALARTSLQRCGVAPSALDTATAA
                     TTITARTPVTGDDLFDFPAARAAQVEGAIDAAHAAFLQWRTVPAPIRGQLIKRLGELL
                     TEHKSDVANLISIEVGKISSEALGEVQEMIDICDFAVGLSRQLDGRTMPSERPGHRLM
                     ETWHPLGVVGVVSAFNFPAAVWSWNTAIAIVCGNAVVWKPAQLTTLTAAACSALFARA
                     AHAVGAPESLNVLVSASAADTQALIDSPKVPLISATGSERMGEQIAPRIAARFGRAIL
                     ELGGNNAAIGTPSADLDLATRGIVFSAAGTAGQRCTTMRRVIVHEDIADELVRRLTEV
                     YARLPIGDPFAAGTLVGPLIDDRAYANMTAALEQAQAQGGTVLVGGGRRESTADSYYV
                     EPAIVQMPRQTEVVKAETFAPILYVLTYSTFEEAIALHNEVPQGLSSAIFTLDQREAE
                     RFIAADGSDCGIVNVNIGTSGAEIGGAFGGEKTTGGGRESGSDAWRAYMRRATNTVNY
                     SSELPLAQGVSFA"
     gene            11735..12865
                     /locus_tag="VFC01_01785"
     CDS             11735..12865
                     /locus_tag="VFC01_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HZN78406.1"
                     /translation="MFNLIDEWGPEKIVCVSDARTQMRGVLVIDNTARGMGKGGTRMS
                     PTVSVAEVARLARNMTWKWAGVDLFYGGAKAGIQADPSAASKDDVLRAFVRALRNEVP
                     EEYVFGLDVGLTETDAAIMLDEVGNRAGVVGTPHALGGLPYDQLGVTGYGVAETVDAA
                     AQFVGASTSKLSVSIQGFGAVGAATARRLDDLGATVVAVSTSEGGLYNPDGLDVAELL
                     ALRTQHGDALVHHYFDAKPLIAGEELAIAADVLVPAALQDVIDAELAHIVPARIVVEG
                     ANLPSSPAAQQILHDRGVIVVPDFIANAGGVVAAAVAMDYRFSGIRPEPAAVFDNISA
                     KLRACTQEILHESRQRGMTTHRVARAAAQVRVAEAMRLRGRR"
     gene            12862..14028
                     /locus_tag="VFC01_01790"
     CDS             12862..14028
                     /locus_tag="VFC01_01790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN78407.1"
                     /translation="MTKTVPERASVVVIGGGVIGSSIAYHLASSGVSDVVLVERDELA
                     CGSTCRAAGGVRASFSNPANIEIGLRGLEVYSTFADEYGQEIDFRRDGYLYTLSDRHN
                     LEVFTESVALQNSHGVPSRIIDPEEAQRISPLISTEGMVGACWSPMDGKATPESVVMG
                     YAAAARRHGARIIRHCEVLDIASDAGTIAEVLTAHGRIKTNTVVCAAGAWSRRIGDMV
                     GVTIPVTPVRRQIAFTEPVAELPATSPSLTIDFPSNFYFHPEGKGLLLGWSDPNEVEG
                     FNLRFELEDWLVGLGAIAEKRAPAVLDYGIASGWAGLYEMTPDRNQIIDRSAEVEGLL
                     IATGYSGHGFLMGPATGEIVRDLYQGVEPKYDISGFTLSRFSDVDLRSGETNIV"
     gene            14054..15229
                     /locus_tag="VFC01_01795"
     CDS             14054..15229
                     /locus_tag="VFC01_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN78408.1"
                     /translation="MSQTTVTPDELFAIDSLLTGEEREIRDSVRGLVQRIIAPHIASW
                     YERGELPVRDLATELGSLGLLGMHLHGYGCAGTTSLAYGLACLELEAGDSGIRSLVSV
                     QGSLAMFAIHRYGDEDTKQQWLPRMATGEAIGCFGLTEPDFGSNPAGMRTLAKRDGDD
                     WVLNGTKMWITNGSVADVAVIWARTDDGIRGFVVPTATPGFTTHAITSKMSLRASVTS
                     ELVLDDVRLPAAAELPGATGLSGPLGCLNEARFGIVFGALGAARDCLHTALAYAETRE
                     QFSKPIAGFQLTQDKLAAMALEYGKGLLLAIHLGRSKDASTLAPHQVSVGKLNNVREA
                     LNIARTARTILGANGISAEYPIMRHANNLESVLTYEGTSEMHTLIIGQALTGHPAFA"
     gene            15232..17433
                     /locus_tag="VFC01_01800"
     CDS             15232..17433
                     /locus_tag="VFC01_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZN78409.1"
                     /translation="MAEPIDEYFTATLLAGDEGNGHTSRDDHAPLVAGSTLTVARTLQ
                     IFDSQLGSRHLDLAARALRAEGRGYYTIGSSGHESNATVAAALRPTDPALLHYRSGGF
                     FLERARQVGGTDPLRDVLLGIVAATEEPISGGRHKVFGRADLHIIPQTSTIASHLPRA
                     VGVAFWIARAHKIADTCPWPDDAITVCSFGDASANHSTAVGAINAAINAGYQGVAMPL
                     LFVCEDNGIGISTRTPDEWIATTYANTAGLAYFAADGGDLAATYATAFAAAEWVRHHR
                     RPAFLHIRTVRLMGHAGSDFEPAYRTQRDIIRDYARDPVLATAQLLIAHRHRSATDIV
                     DLYEHKRATVRGLAKEVTQLPQLNSATAVTEPLTTGLREAEHASHIAPAKRRGESLTL
                     AQAINTALGDILDTYPQAILFGEDVARKGGVYGLTRGLQKKFGPTRVFDTILDEQTIL
                     GLALGAGLSGMLPIPEIQYLAYIHNAIDQIRGEGATLPFFSNRQYRNPAIIRIAGYAY
                     QKGFGGHFHNDNSVAALLDIPGIVVASPSRPDDADAMLRSCALAAVHTGALCLFLEPI
                     ALYHTRDLYEEGDGQWLSKPSDEADTTIGTARTHGDGADLTIITYANGVAMSLRVAEK
                     LRCTHGIDARVVDLRWLAPLPIEDILRESRASGRVLVADETRRSGGVGQAVLAALIEN
                     GFQGNVMRVASDDSFIPLGDAALHVLLQERTIEQAGLALCRPKQLARTELQ"
     gene            17453..18685
                     /locus_tag="VFC01_01805"
     CDS             17453..18685
                     /locus_tag="VFC01_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN78410.1"
                     /translation="MYDLAPVAIGQHVQSIVDVLAQSLDRAVLLDDESLAPITHSRQF
                     GQLDDVRMYSLLQRGILPEVKARLFSIGIGTATEAFWTPALPQHAMMSRFCVPICSAT
                     ERFGYLWVLDPERSLSDDGQELARQAGRDLQVVLDRRNADLRAAESYLQALLAQLLEN
                     GTGATSEQILQELQEKDVAQPDSRVSVFAFEPNVAGPSDPVQRTMSLRLRLIATEPSH
                     LWFAVAGQPTAIIAVSRPENRVNAQHVPPAVVRAIEAVYGRRPVIGWSGERVPISQAA
                     QAFRNARLALTLAEIGVSENKVAVWTELGSWRTLAILADSYSKNPKDLAELVHPGIVD
                     MVEGGRDDLVHTLDAYLTHGCDARKTAESLHLHRSTLYYRLEKITEAVGGDLRDGEAR
                     FELMLSIRLANIARLYHI"
     gene            18938..>20396
                     /locus_tag="VFC01_01810"
     CDS             18938..>20396
                     /locus_tag="VFC01_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007301794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN78411.1"
                     /translation="MSATPTLKKALSQRQLTMIAIGGVIGAGLFVGSGVVIGDTGPGS
                     FITYALAGVLIIMVMRMLAEMAVANPSTGSFADYSRNALGNWAGFSVGWLYWYFWVIV
                     VGFEAIAGAKIIQYWIDVPLWLSALILMILMTGTNLFSVSSFGEFEFWFAGIKVAAIV
                     VFIGLGSLFVLGLWPNKSLDFSNLWAHGGFFPMGVMAVTVGVVTVIFSMVGAEIATIA
                     AAESSDPERAVSKAANSVITRIAVFFVGSTFLLAVILPWNDEKAGASPFVSAFTKMGI
                     PYADHIMNAVVLTAVLSCLNSGMYTASRMLFVLAARREAPAQLVQVTRRGVPAIAILA
                     SSVVGFLCVIAAAVSPDTIFAFLLNSSGAIILFVYLLIAVSQIVLRYRTPDSELRVKM
                     WLFPVLSIITAVAIVAILVQMGLQADVRSQLVLSLLSWAVVIVLFLLNKWYVNSRPVV
                     EGVAPTERPHRVLVLANQTVGSAELLDELRRIGADK"
BASE COUNT         3645 a         6968 c         6387 g         3396 t
ORIGIN      
        1 gcgcgactgg gccaatcgat caccgccgaa cagcaggcgg cgggagttgt gccggacagc
       61 cctgcggcgc tcgtcacgct cgccgcgctg cacggcggcg acccggtgcg agcccgcagc
      121 gtgatcggcc gggccgtgcg ggccgttccc gaggaagaac acttcgtcag ccaccggtac
      181 cgactgctgc tcggctgggt gcggatgcag gacggccagc tgtccgcggc cgctgccgac
      241 gttgcgtcga cggccgacgc cacgctgcat cgccgcgacg cactctgggc cgccgcgttg
      301 caaacggcaa tcgccaggcg cagcggcgac agcggcgcgg tgcagaagca ttggtacgcc
      361 gcgatggaag tgctcgccga gtactcgatg gacctctttg ctttgttgcc gctgggcgag
      421 ctgtgggtgt ccgctgccag gatgcgtcag gtcgaccgct tgcggcacac cttgaccgaa
      481 tcgttcgcgc tgctggaatc gctcggcaat ccgctgctgt ggtcggtgcc gttgcactgg
      541 gcaggcgtac acgccgggat cctggccaat tcaccggaag cggtcgcgcc acacggccag
      601 gcgttgaccg cagcggcggc gcacagcccg ttcgcgaagg cgctggccac cgccgggcgc
      661 acctggctgc gggtgctggc caatcacgtc gacatcgacg aggtgaccac ggccgcgcgt
      721 ggcctgtcgc agttcggtct gacttgggac gccacccggt tggccagcca ggctgctttg
      781 cagactcctg acggccgcgt gtccagcgcg atgctgcagc tggcccgcga cctcaagcag
      841 accgccgcgg tcgacgaggc gccgggcctc gaaccggcca gcaccgtcac cgagcgcacc
      901 ggcccatctc ggcccgcgtc ctcccggttg tccgaccgcg agcgggaagt ggccgagctg
      961 ctgctgctcg gcattcccta ccgcgacatc ggcagccagc tgttcatctc ggccaagacg
     1021 gtcgagcacc acgtcgcgcg gatccggcgc aggctcggcg cggagtcgcg gtccgagatg
     1081 ttgtcgatgc tgcgcgccat gctggcgcct cagggttaac cccccaacgc agcggtttcc
     1141 cctaatcgaa atcccctatc ggggtcgtac agcgatgggt gcggcaccgg atttgctgtg
     1201 ccgcggatcc ccatacggtc gttcgcatga actccccagg caaccacggc cgttcactgg
     1261 gcctgtcggt cggcgcgacg aacctcgccg gcatccgcga cgggcagagc gccgtcatcc
     1321 gtcccgccga gctgacgctg cacggccagc gcctcaccgg cttcgtcgac cgcgtcggcg
     1381 atccggtgcc gctcatcgcg cccgacggct cgaagcatcg gtccgaagaa ctgctcgccg
     1441 atgcgctcgg cgcgatggcc catgcggtca ccgacggcat gccggtctcc gacgtcgccg
     1501 tcaccgtgcc cgcgcactgg cgcgcatcgg tcgtcgacac cctgcgcagg aacctgcgcg
     1561 gcaggttgcc ggtggtctcc gacgcgaccg cggcgctgac ggcgttgcgg gccaatcccg
     1621 gcctgccgac gcgcggtgtc atcgtgctgt gcgacttcgg cggcagcggc accagcatca
     1681 cgctggccaa cgcggcgcgc gactttgcgc cgttcggcga gaccgtgcgc ttgcccgact
     1741 tctctggtga ccagatcgat caggcgctgc tgacgcacgt cgtcggcgga ctcgccgaga
     1801 ttgatccgtc cggcaccgcg atggtcggct cgctggtccg gttgcgcgac gaatgccggc
     1861 gcgccaagga acggttgtcg gcggagacgg cgactgccgt cgtggccgag ctgcccggcc
     1921 agcgtgccga catccgggtc acccgcaccg agctcgagga cctgatggcc gcgccgctgt
     1981 cggaattcct tgccgcgctt gacgaaacgc tcgagcggta cggcgtgcct gcggcacacg
     2041 tgtcggcggt cgctaccgtc ggcggcggtg cccgcatccc tttgatcacc cagcggctct
     2101 ccgaacacct gcgcgcaccc gtcgtgacca cacctcatcc gcagctgacg gcggccgagg
     2161 gcgcggcgct gatcgcacac cgcgcccggg tcgtcgacac cgcgaccacg ctgacgccga
     2221 ccgttcctgc ggctgcggcc gcgaccgcgg ctggggacgc gaccgtgtcg ctggtggcgc
     2281 acgagcaggc gctggcctgg tccgaggatg acgccagcga ggacgtgctg ccctacaacg
     2341 acgtgaccga ctcgcgcccc gaggtgcact tccgccacga ggaatggcag gacgacgaac
     2401 cgccgcggcg tggcccgctg gtgctgttcg ggttggcggc ggtcgccgcg accatcgccg
     2461 ccgcggtgtt cggcttcacg ttgctcagcg acgccaccac gaccccggtc gatgcggcgg
     2521 tcaccgccac gtcgaccgtc ccagcgcctg cggcgtcggc gcctctccca gcaccaccgg
     2581 cgccgcaggc cccccaacaa gtgacgactg tcgtggtgca gaagcccgcg cagcggtcgt
     2641 atccgcaggc gccggtggcg cagcaaccgc ctgcacccgt gacgacggaa ccgacgacca
     2701 cgactccaac gacgaccacc ccgacgacga caacgacgac gccgacgacc acaacgacga
     2761 ccaccacgac gccgccgcca ccaccgccga tcgacccggg cccaggtggc ggaaccggta
     2821 cctccacacc gccgatcgac cccgcgacag gccccggagc gtagttaggg cagccttcgt
     2881 agcggcgttt ccccgcaaga tgcaactatg agcaggcatc gagcgaaagt tactggtcag
     2941 taacatgtac taagttaccc ttgggtaact taacggatgg ggaggcaccc ggtggatacc
     3001 cagatgctca tcaggctcgt cgtcgggctt ggactgacgg cgctagtgct ggtgttcgcc
     3061 gccaagcggg tgctgtggct gacgaccctg attcgctccg ggcagaagac gagcgtcgag
     3121 aacaatcgca aagaccatct gggcaagcgg atcacgacgc aggtcgagga agtcttcggg
     3181 caaacccggc tgctgaagtg gtcgatcccg ggcatcgcgc acttcttcac aatgtggggc
     3241 ttcttcatcc tcgcgtcggt ctacctcgag gcctacggtc tgctgttcgt ccacgacttc
     3301 cacatcccgt tcatcggccg gtgggacggg ctcggattcc tgcaggactt cttcgcggtc
     3361 gcggtgctgc tcggcatcgc cactttcgcg gtgatccgca tcgtgcgcga gcccaagaaa
     3421 cacggccgtg attcccgctt ctacggatcg cataccggcg gcgcctggct gattctgttc
     3481 atgatcttca acgtcatctg gacgtacgca ctggttcgcg gttcggcggt gaacaccggc
     3541 aacctgccct acggcaacgg cgccttcttc tcccagttga tgggttgggt gctgaatccg
     3601 ctcggccaca ccgccaatga atggatcgag accgtcgcgc tgctgctgca tatcggcgtg
     3661 atgctggtct tcctgctgat cgtgctgcac tccaagcacc tgcacatcgg cctggcgccg
     3721 gtcaacgtca cgttcaagag gctgcccgac ggccttggcc cgctgctgcc cgtggagtcc
     3781 aagggcgagg cgatcgactt cgaggatccc gccgaggacg cggtgctggg ccggggcaag
     3841 atcgaggact tcacctggaa gggctacctc gacttcacca cgtgtaccga gtgcggccgc
     3901 tgccagtcgc agtgcccggc ctggaacacc ggaaagccgt tgtcgcccaa gctcgtgatc
     3961 atgaacctgc gcgaccatct gttcgcgaag gcgccctaca tcctcggcga taaggagacc
     4021 ccgctcgaga acacgccgga aggcggcctc ggcgaggaac tcggcggcga gaagcacgac
     4081 gaggagcacc acgtccccga gtccgggttc gaacgggtga tgggctccgg tcccgcacag
     4141 gccacccgcc cgctggtggg taccgccgaa cagctcggcg tcatcgaccc cgacgtgctg
     4201 tggtcgtgca ccacgtgcgg tgcgtgcgtc gagcagtgcc cggtggacat cgagcacatc
     4261 gaccacatcg tcgacatgcg ccgctaccag gtgatgatgg agtccgagtt cccatccgag
     4321 ctcggcgtgc tgttcaaaaa ccttgagacc aaaggcaatc cgtggggcca gaacgccaag
     4381 gaccgcacga actggatcga cgaggtcgac ttcgacgtgc cggtgtacgg caaggacgtc
     4441 gatagcttcg agggctacga gtacctgttc tgggtgggtt gcgcaggagc gtacgaggac
     4501 cgcgcaaaga agaccaccaa ggccgtcgcg gaactgctcg cggccgcagg agtgaagtac
     4561 ctggtgctcg gcgagggcga aacctgcaac ggcgactcgg cacggcgctc gggcaacgag
     4621 ttcctgttcc agcagctggc cgcccagaac gtcgagacgc tcaacgacct gttcgaaggt
     4681 gtggagcggg tggaccgcaa ggtcgtcgtc acctgcccgc actgcttcaa cacgctgggc
     4741 cgcgagtacc cgcaggtcgg cgggaattac accgtgctgc accacacgca gttgctgaac
     4801 cggctgatcc gcgacaagaa gctggtgccc gtgagtcctc cggacggcgg caaggacatc
     4861 acctaccacg acccgtgcta cctgggcagg cacaacaagg tctacgaggc gccgcgtgaa
     4921 ctgatagggg cctcgggcgc caagctgacc gagatgccgc ggcacgccga ccgcggcctg
     4981 tgctgcggcg cgggtggagc gcggatgtgg atggaagagc acatcggcaa gcgcgtcaac
     5041 cacgaacgtg tggaagaggc gctggacacc ggtgcatcgg ccatcgcgac cggctgcccg
     5101 ttctgccgcg tgatgatcac cgacggcgtc gatgacgtcg ccgcggccaa cgacgtgccg
     5161 aaggcggagg tgctcgacgt ggctcagctg ctgctgggct cgctcgacaa gaccggcgtc
     5221 accctgcccg aaaaaggcac ggccgcaaag gaagccgagg agcgcgccgc gaaggccgca
     5281 ccgaaagcag aagccgccac cgcggtcgcc gagccggaag ccaagccgga gccggcggcg
     5341 gccgcccccg cgactcaggc tgaatcgccg gctcagccgg cgggaacgaa gcccgtcacc
     5401 gggctgggca tcgccggtgg cgcgaagcgt cctggagcaa agaagacggc ggcgtccact
     5461 gccgaagcac cggctgcacc cgcagcgcct gcgaaggggc tcggcatcgc cgccggcgcc
     5521 aagcgtcccg gcgcgaagaa ggccgcaccg gccgcacccg cagctgccgc taccgaaacg
     5581 tccgctccgg cggcgccggc caagcctgaa cccgaggtca agggactcgg tatcgccgcg
     5641 ggagcacgcc gtccgggcgc caagaagact gccgccgcgc ccgccgagcc agcctcgacg
     5701 gcgacggaac cggcatcctc ggccaccgaa cctgcgtcca ctgcgaccga gccgcccgtc
     5761 aagggcctcg gtatcgcgcc aggcgctcgt cggccgggtg cgaagaaggc cccagcggca
     5821 ccgaaacccg ttgtgcccca gccggaaccg gcctccgcga gcgaatcagc tgctgcgcct
     5881 gagccttccg ccgacgacaa cggcgagaca cccgcgccgc cggtgaaggg ccttggcatc
     5941 gccaagggcg cgcggccgcc aggcaagcgg taatcggcag gtgtcgcctg cgggctacag
     6001 ggcccgcatg agccgctcat ccttgggata ttgcgtcatt tcgcgaatat cttcgaccag
     6061 tcccgtggga ccgacctttt cgcgacgctg tgccagctgc ggctgcatct cgtcgggtat
     6121 cagctcgagc cgggtcgggg ctttccacag ctcgatggtc tggatgatcc agtaggcagc
     6181 gaactccagg atcaacgcgg actccaaggc gatgacccag tggttccatc cccatgccaa
     6241 atgcacggcg atgacaagta gcaatgtcac gcccatcaac gcggccacaa tctggtaggc
     6301 ccgttggtag gcgcccttgt gcggcgactg ctgcttgctc accaagaacg cggtgatcac
     6361 gacggtgatg atgatggcga tgaacatgac cgccgcggcg acaccgtggg cgtggtcgta
     6421 gaacaaatcc cgaaagaaga tgaagccgac caaacccacg gctatcacca aacgcaaggc
     6481 ccacacggtg atttcgccga gaagactgcg atggccgacg ccgccggcgc gccagtagag
     6541 ccaccacgcg accacctggg cgcccgccat ggcgatgacc accgcccaga cgttgttggt
     6601 gatcgacagt ccggacgtca cagccggcgg cgtctcaccg cacaggcgct caggcagccg
     6661 ggtcggcacc agcgcgacga cgaacgccag aatgcctgcc aggttcagca aattgtcttc
     6721 ggtatcggtg ctgcctttgt agacgattag ctgcgcgcca atcgcgcaca atgcggcgac
     6781 gaagatggcg tgcgccgacg tgtagtaata ggcgctgatc gaaccttgcc agcagctagc
     6841 gctgagccgc tcaccgatta ccgatgcgcc aagcatgact atcatgacag ccataccgcc
     6901 tcgcaggtag cggtaggtgt cgaggaagaa atcgcgcgac tgtacgtctg cggtgtgttg
     6961 ttgcgtactc atgggccccc aacccggtga atgaactgtc ggtttgccgg cttgttgatt
     7021 atgacaagaa atagctggca ctgggtgaat atcgatgaaa cccgaaccac gtggcaaatt
     7081 cgctagacgg caatgcgatc ataggtggcg tgactaccca ccacgtgccg tggcagacca
     7141 cgggacatca cccgaagcag cggacgttca cgcagtcgtc gaagctgcag gatgtcctgt
     7201 acgagatccg cggcccggta cacgaacatg cgtcgcggct ggagtccgag ggtcatcgca
     7261 tcctcaagct gaacatcggc aatcccgcgc cgttcggctt cgaggcgccc gacgtgatca
     7321 tgcgcgacat catttcggcg ctgccgtacg cgcaggggta ttcggattcc aagggcatca
     7381 tgccggcccg tcgcgcggtg ttcacccgct acgaactcgt cgagggcttc cccaagttcg
     7441 acgtcgacga cgtctatctc ggcaacggcg cctccgagct catccagatg acgttgcaag
     7501 cgctgttgga caacggcgac caggtattga tccccgcacc ggactatccg ctgtggaccg
     7561 cgtgcacctc gctggcgggc ggcacgcccg tgcactatct gtgcgacgag acccagggct
     7621 ggatgcccga tatcgccgac ctcgagtcga agatcaccga tcgcaccaag gcgatcgtcg
     7681 ttatcaaccc gaacaacccg accggcgcgg tatacacccg cgaaatcctc acgcagatcg
     7741 ccgatctcgc ccgcaaacac gaactgctac tgctgtccga cgagatctac gacaagatcc
     7801 tctacgacga cgccaagcac atcgcgacgg cgtcggtcgc gccggacgtg ctgacgctga
     7861 cgttcaatgg tttgtcaaag gcctaccggg tcgcgggtta ccggtcgggt tggctggtca
     7921 tcaccggccc caaggagcac gcaagcagct tcatcgaagg catcagcctg ctcgccaata
     7981 tgcggctgtg cccgaatgtc cctgcgcagc atgccatcca ggtcgcgctg ggtggtcatc
     8041 agagcatcga ggacctggtc ctgcccggtg ggcggctgct cgagcagcgc gatgtggcct
     8101 gggagaagct caacgagatc cccggcgtgt cctgtgtgaa gcccgagggg gcgctgtaca
     8161 cgttcccgcg gctcgacccc gaggtctacg acatccagga cgacgagcaa ctcgtcctcg
     8221 acctgctgct gcaggagaag atcctggtta cccagggcac cggcttcaac tggccgacgc
     8281 cggatcactt gcgcatcgtg acgctgccgt ggtcgcgtga tcttgccaac gccatcgagc
     8341 ggctgggcaa cttcctgtcg tcctaccggc cttaactacc cgatccggcc gagcaggaag
     8401 tcgtcgaaca cgttgtcgtg ttcaacggcg aaggcctctt cgaatgtcgc actggcactt
     8461 ccaatgagat agcgcttggt ggtttcgagc gcggcctgcg gcgcctcgat gatcgaccgc
     8521 gccatcgcca acgcaccgtc gagtaggtca ccgggttcgg tgacacgggt gacgaggccg
     8581 atgcgtaatg cctctgcggc atcgatccgg cgtcccgtca ggcaaagttc gcgcgccacg
     8641 ccgagaccga cgatccactg cagcggcgtg aacagcggcg gtgcgccgaa tttgatctct
     8701 ggatgcccga atgccgcagc gctggaagcg attcggcagt cacacagcac gccaaggtcc
     8761 attccgccgc ccatcgcggg cccgttcacg gcggcgatca acggcttggg aaaatgccat
     8821 acggcgaggt ggtaccggcg cgagctgtcc tttatctgcc gcgcgagatc ggcttgaccg
     8881 aattcgttga ggtcgaagcc ggcgcagaac gtcggccccg ccccggtcag cacgaccgcg
     8941 cgaacagccc catccgccgc ccagtcgtcg agctgctgtg tgatctcatt gcgcaacttg
     9001 attgacagcg cgttccgccg ctgcggccgt gccagcgtca gcgtcgccac cccgtcgtcg
     9061 gctacctgcg tctcgatatc cctactcatc gccttgatct aaatcacgcc gagctcgccc
     9121 gtctaccccg ttgcgcgtga ggtcacccgc ggtcgctgat gacgtgttga acgatctccg
     9181 taaccgcccg caccgtggga cgggcgtcgg cacccgccgg tcggatcagc acgatcttgc
     9241 gcacaatctc ctgcttcagc gcgatccgga tgatcggcac ggagctgttc agtccgatca
     9301 tcatgtcggt gaccggcgcg atgcccaatc ctttggccgc cagcgcgagg gtgactgcgg
     9361 tgtcgatgat ctcgtgagac gtcgccggtt cgaaaccatg ttgccgacac acggtgcgca
     9421 ttgccgcgcc gtagtgggtg tctgacggcg gcagaatcca atcccagtcg gcggcgtcgc
     9481 gaagatcgat gcggctctcg gtgcgcatgc cgtaggcccc cggggatacg gcaagtccga
     9541 accgctcctc gcgcagagtg atgatgtcga cgccgggcaa ccggggcagc ggcacactcg
     9601 gatagtccag cccgaaggcc gcgtcgacgc ggccgcgcgt caccgcgtcc agtgcgtcgt
     9661 cgacgtcgag ttcgatgctg cgcagccgca tctgcggata ggccgcgtgc gcgcgggcca
     9721 ccaccgcggg cagtagggcg gctgccgtgc tgccaaacgt ccccagcaat accggtgcgg
     9781 cgacgtggtc gcggacattg agcaccgcgt cgagagccgc ccgctcggcg gccagcaccc
     9841 ggtcggcgtg ttcggccagc acggtcccgg cgtcggtgag cacgaccttg cgtccgacct
     9901 ttcgtgatca gtcgcgtgtt caccgacttc tccagggcgg cgatctgttg cgacaccgcc
     9961 cccggcgtgt atcccaactc atcggcgacg gcgaccatcg tgccgcggcg cgcgagctca
    10021 cgcagggtgg tgagctgccc gaggttcaga ttcatataga aactctaaag taacgagcgc
    10081 actttctttc gcttgttcta ttcgttcctc ggcggcgacg atgtggtcat ggccacacct
    10141 gcaatgagca cctcaatctc cacgatcacc gaaaccgtct gcttgcccag caccgatgag
    10201 ctacgtgcgt tggcccgcac cagcctgcag cgctgcgggg tcgcccccag cgcgctggac
    10261 accgctaccg ctgcgacgac gatcaccgca cgcacgccgg tcaccggcga cgatctgttc
    10321 gatttccccg ctgcacgcgc tgcgcaggtc gagggtgcga tcgatgccgc tcacgccgcg
    10381 ttcctgcagt ggcgcaccgt gccagcgccg attcgcgggc agctcatcaa gcgcctcggc
    10441 gagttgttga cggagcacaa gtccgacgtc gccaacctca tcagcatcga ggtcggcaag
    10501 atctcctccg aggctctcgg cgaggtccag gagatgatcg acatctgcga cttcgcggtc
    10561 ggcttgtcgc gccaactcga cggtcgcaca atgccgtcgg agcgtccggg gcaccggctt
    10621 atggagacct ggcatccgct cggcgtcgtc ggcgtggtct cggcgttcaa cttccccgcc
    10681 gcggtgtggt cgtggaacac cgcgatcgcg atcgtctgcg gcaacgctgt ggtgtggaag
    10741 cccgctcagc tgaccaccct caccgccgcg gcgtgctcag cgctgttcgc ccgcgccgcc
    10801 cacgcggttg gcgcgccaga gagcctcaac gtccttgtgt ccgcaagtgc cgccgacacc
    10861 caggccctga tcgacagccc caaggtgccg ctcatcagcg ccacgggctc cgagcggatg
    10921 ggcgagcaga tcgcgccacg catcgctgcc cgattcggcc gcgccatcct cgaactcggc
    10981 ggcaacaacg ccgccatcgg gaccccctcg gccgacctcg acctggcgac ccgcggcatc
    11041 gtgttctccg ctgccggcac cgcgggtcag cgctgcacga ccatgcggcg ggtgatcgtg
    11101 cacgaagaca tcgccgacga actcgtgcgc cgcctgacgg aggtctacgc gcgcctgccc
    11161 atcggcgatc cgttcgccgc gggcaccttg gtaggaccgc tgatcgacga ccgcgcctac
    11221 gccaacatga ccgccgccct cgaacaggcc caggcgcaag gcggaaccgt tctggtcggc
    11281 ggtggccggc gcgagagcac tgcggattcg tattacgtgg aacccgcgat cgtgcagatg
    11341 ccccgtcaga ccgaggtcgt caaggccgag acgttcgcac cgatcctgta cgtcctgacc
    11401 tactccacgt tcgaggaagc gatcgcactc cacaacgagg tcccgcaggg actgtcgtcg
    11461 gcgatcttca ccctcgacca gcgtgaagcc gaacggttca tcgccgcgga cggctcggac
    11521 tgcggcatcg tcaacgtcaa catcggcacg tccggcgccg agatcggcgg cgcgttcggc
    11581 ggcgagaaga ccaccggtgg cggccgcgaa tccggatcag atgcctggcg ggcctacatg
    11641 cgtcgcgcca ccaacaccgt caactactcc agtgaactgc cgctggctca aggcgtcagc
    11701 ttcgcctgag cgacgaaaga cggggattca caccatgttc aacctgatcg acgaatgggg
    11761 tccggagaag atcgtctgcg tctccgacgc caggactcag atgcgcggcg tgctcgtcat
    11821 cgacaacacc gcacgcggaa tgggcaaggg aggcacccgg atgtcgccca ctgtcagcgt
    11881 cgccgaagtc gcccggttgg cccgcaacat gacctggaag tgggcgggcg tcgatctgtt
    11941 ctacggcggc gccaaggccg gcatccaggc cgatccgtct gccgcctcca aagacgacgt
    12001 gctgcgtgca ttcgtacggg cgctgcgtaa cgaagtgcca gaagaatatg tcttcggcct
    12061 cgacgtcggt ctcaccgaaa ccgacgcggc gatcatgctc gacgaggtcg gcaaccgcgc
    12121 cggtgttgtg ggaacccccc atgcgctcgg cgggttgcca tacgaccagc tcggggttac
    12181 cgggtatggt gtagccgaaa cagtcgacgc cgcagcgcaa ttcgtcggtg cgtcgacctc
    12241 gaagctgtcg gtcagcattc agggcttcgg tgcggtcggc gccgcgaccg cacgccggct
    12301 cgatgatctc ggagccacgg tggtcgccgt gtcgacatcc gaaggcggcc tatacaaccc
    12361 cgacgggctc gacgtcgcag agctcctggc gttgcgcacc cagcacggcg atgcgctggt
    12421 gcaccactat ttcgacgcca aaccactgat cgccggcgag gaactcgcca tcgccgccga
    12481 cgtgctcgta ccggccgcgc tgcaggatgt catcgacgcc gagctcgctc acatcgtgcc
    12541 ggcccgcatc gtggtcgaag gcgcgaacct gccgagcagt cctgcggcgc aacagatcct
    12601 gcacgatcgc ggggtgattg tcgtacccga cttcatcgcc aacgccggcg gggtggtcgc
    12661 ggcagccgtg gcaatggact accggttctc gggcattcga cccgaaccgg ccgctgtgtt
    12721 cgacaacatc tccgcgaagc tgcgcgcctg tacacaggaa atcttgcacg agtctcgaca
    12781 gaggggcatg accacccatc gcgtcgcccg ggccgccgca caggtccgcg tcgccgaggc
    12841 aatgcggctg cgaggtcgac gatgacgaaa accgtccccg aacgcgcatc cgtcgtcgtc
    12901 atcggcggtg gcgtcatcgg atccagcatc gcctaccatc tggccagctc cggcgtctcc
    12961 gatgtggtgc tggtcgaacg cgacgaactc gcctgcggct caacatgtag ggccgcgggc
    13021 ggcgtgcgcg cctcgttcag caaccccgcc aacatcgaga tcggcctgcg cggtctggag
    13081 gtttactcaa ccttcgccga cgagtacggc caagaaatcg acttccgccg cgacggctac
    13141 ctttacacac tgtccgatcg gcacaacctt gaggtcttca ccgaaagcgt ggcgctgcag
    13201 aacagccatg gcgtgcccag ccgcatcatc gatcccgaag aagcgcaacg catttcaccg
    13261 ctgatcagca cagagggcat ggttggagcc tgttggtcac ccatggacgg caaggccacc
    13321 ccggagtcgg tggtgatggg ctacgccgct gccgctcgcc gccacggcgc gcgaatcatc
    13381 cgtcactgcg aagtcctcga catcgccagt gatgcgggca ccatcgccga ggtcctgacc
    13441 gcacacggtc gtatcaaaac caacacggtc gtctgcgccg ccggggcgtg gtcacgccgg
    13501 atcggggaca tggtcggcgt gaccatcccc gtcaccccgg tgcgccgcca gatcgcgttc
    13561 accgaaccgg tcgccgagct gcccgccacc tcgccgtcac tgaccatcga cttcccttcg
    13621 aatttctact tccatccgga gggcaagggc ctgctgctgg gctggtcgga ccccaacgag
    13681 gtcgaaggct tcaacttgcg cttcgaactc gaagactggc tcgtgggtct gggcgctatc
    13741 gccgagaagc gcgccccggc cgtgctcgac tatggcatcg ccagcggctg ggcgggcctg
    13801 tacgaaatga ctccggaccg caaccaaatc atcgaccgca gtgcagaagt cgaaggcctg
    13861 ctcatcgcca ccggctactc cggacacgga ttcctgatgg ggccggcgac cggcgagatc
    13921 gtgcgcgacc tctaccaggg cgtggaaccc aagtacgaca tcagcggatt caccctcagc
    13981 cggttcagtg acgtcgacct ccgatccggc gaaaccaaca tcgtctaaca ccacctgaca
    14041 gaggaacctt cgcatgagtc agaccaccgt cacacccgac gaactgttcg cgatcgactc
    14101 gctgctcacc ggcgaggaac gcgagatccg cgacagcgtc cgtggcctcg tgcaacgcat
    14161 catcgccccg cacatcgcca gctggtacga acgcggcgaa ttgccggtgc gcgacctggc
    14221 caccgaactg ggctcactgg gcctgctcgg tatgcacctg cacggttacg gctgcgccgg
    14281 aacaacctcg ctggcttatg gactggcctg cctggaactg gaagccggcg attcgggcat
    14341 ccggtcgttg gtcagcgtgc agggatcact agcgatgttc gcgatccacc gctacggcga
    14401 cgaggacacc aaacagcagt ggctgccacg catggcgacc ggcgaagcga ttggctgttt
    14461 cgggctcacc gaacccgact tcggctctaa ccctgcggga atgcgcaccc tcgccaaacg
    14521 cgacggcgac gactgggtgc tcaacggcac caagatgtgg atcaccaacg gttccgtcgc
    14581 cgacgtcgcg gtcatctggg ccagaaccga tgacgggatc cgcggattcg tcgttccaac
    14641 ggccaccccc ggcttcacca cgcacgccat cacctccaag atgtcactgc gggcctcggt
    14701 caccagtgag ctcgtgctcg acgacgtccg actgccggcg gcagccgaat tgcctggcgc
    14761 cacagggtta tccggaccgt tgggttgcct gaacgaagcg cggttcggca ttgtgttcgg
    14821 cgcgctgggc gccgcccgcg actgcctgca cactgcgttg gcctacgccg aaacccgtga
    14881 acagttctcc aaaccgatcg ccggattcca gctcactcag gacaagctgg ccgcgatggc
    14941 gcttgagtac ggcaaaggcc tactgctggc gatccacctc ggcagatcca aagacgcaag
    15001 cactttggcg ccacatcaag tcagtgtggg caagctgaac aatgtccgcg aggcgctgaa
    15061 catcgctcgc acagcacgaa ccatactggg cgccaacgga atcagcgccg agtatcccat
    15121 catgcggcac gccaacaatc tcgaatcggt attgacctac gaaggcacca gcgagatgca
    15181 cacactcatc atcggccagg ccctaaccgg acaccccgcc ttcgcatgac catggccgaa
    15241 cccatcgacg aatacttcac cgccacactg ctggccggtg acgaaggcaa cgggcacacc
    15301 agccgcgacg accacgcacc gttggtggcg ggctccacgc tcaccgtcgc ccggacgctg
    15361 cagatcttcg actcccagct cggcagccgc cacctcgacc tcgcggctcg cgccctacgc
    15421 gccgaaggta ggggctacta cacgatcgga tcttctggcc acgaaagcaa cgccaccgta
    15481 gccgccgcgc tgcgacccac cgatcccgcg ttactgcact accgatctgg cggtttcttc
    15541 ctcgaacgcg cgcgccaggt cggtggcacc gatccgctgc gtgacgtcct gctcgggatc
    15601 gtcgccgcca cagaggaacc catctccggc gggcgccaca aggtgttcgg cagagctgac
    15661 ctccacatca tcccgcagac ctcgacgatc gcctcccatc tgccgcgcgc ggtgggcgtc
    15721 gcgttctgga tagccagggc ccacaaaatc gccgacacct gcccgtggcc cgacgacgcg
    15781 atcacggtgt gcagtttcgg cgacgcctcg gccaaccact ccaccgcagt tggcgcgatc
    15841 aacgctgcca tcaacgccgg ctatcagggc gtggcgatgc cgttgctatt tgtgtgcgag
    15901 gacaacggga tcgggatcag cacccggacc cccgacgagt ggatcgcgac gacgtacgcc
    15961 aacactgccg gcctggccta cttcgccgcc gacggaggcg atctggccgc cacctacgcc
    16021 accgcatttg ccgccgccga atgggtcagg caccatcgcc gccccgcgtt cctgcacatc
    16081 cgcaccgttc gcctgatggg acacgccgga tccgacttcg aaccggccta ccgcacccaa
    16141 cgcgacatca tccgcgacta cgcccgcgac cccgtgctgg ccaccgctca actgctcatc
    16201 gcgcatcgac accgcagtgc caccgacata gtcgatctct atgaacacaa acgcgccacc
    16261 gtgaggggac tggccaaaga ggtaacccaa ctccctcaac tgaattcggc gaccgcggtc
    16321 accgaaccgc tgaccaccgg gctgcgcgag gccgaacacg cctcacacat cgcaccggcg
    16381 aagcgccgcg gcgaatcgct caccctggcg caggccatca acaccgcgct tggcgatatt
    16441 ctcgacactt acccgcaggc catccttttc ggcgaagacg tcgcccgtaa gggcggcgtg
    16501 tatggactca cccgcggtct gcagaaaaag ttcggcccga cccgcgtctt cgacaccatc
    16561 ctcgacgaac agaccattct cgggctggcc ctgggggccg gcctcagcgg catgctgcca
    16621 atccccgaaa tccagtatct cgcctacatc cacaacgcga tcgaccagat ccggggcgag
    16681 ggtgcgaccc tgccgttctt ctccaaccgc cagtaccgca accccgccat catccgcatc
    16741 gccgggtatg cgtaccagaa aggcttcggc gggcacttcc acaacgacaa ctccgtggcc
    16801 gcgcttctcg acatcccagg aatcgtggtg gcctcaccgt cgcgtcccga cgacgcagat
    16861 gccatgctgc gcagctgtgc actcgccgcc gtgcacactg gagcgctctg cctgttccta
    16921 gaacccatcg cgctctacca cacccgggac ctctacgaag aaggcgacgg acagtggcta
    16981 tccaaaccct ccgatgaggc tgacaccacc attggtaccg cccgaaccca cggcgacggc
    17041 gcagacctca ccatcatcac ttatgccaac ggcgttgcaa tgagcctgcg cgtcgccgag
    17101 aagttgcgct gcacccacgg aatcgacgcg cgagtcgtcg acctgcgctg gcttgcacca
    17161 ttacccatcg aggacatcct gcgcgaatcg cgtgcgagcg gtcgtgtcct cgtggcggac
    17221 gagacccgtc ggtccggcgg agttgggcag gccgtgctag cggccctgat cgagaacgga
    17281 tttcagggaa acgtgatgcg ggtcgccagc gatgacagct tcataccgct cggtgatgcc
    17341 gccctgcatg tgctccttca ggaaaggacc atcgaacagg ccggcttggc tttgtgccgt
    17401 ccgaaacaac ttgcacgcac agagcttcag tagcgatcgg aggtttctga cgatgtatga
    17461 cttggcccca gtggcgatcg gccaacatgt ccagtcgatt gtcgacgtgc tcgcccaatc
    17521 cctggacagg gcggtcctgc tcgatgacga atcccttgcc ccgatcacgc acagccgcca
    17581 gttcggacag ctcgacgacg tccgtatgta cagcctcctg cagcgtggaa tccttccaga
    17641 ggtcaaggcc aggctcttct caataggaat cggtacggcc acggaggcgt tctggacccc
    17701 ggccctgcct caacacgcga tgatgtcgcg gttctgcgtg ccgatctgtt cggcgaccga
    17761 gcggttcggg tacctgtggg tgctcgatcc cgaacgttcg ttatccgatg acggtcaaga
    17821 gctggcaagg caagcgggca gagacctgca ggtcgtcctt gatcgccgaa acgccgacct
    17881 gcgcgctgcg gagtcatacc tgcaggcgct tcttgcgcag ctactcgaga acggcacggg
    17941 tgcgacgtcc gaacagattc tgcaggaact gcaggagaag gacgtagcgc agccggacag
    18001 ccgggttagc gtgttcgcct ttgagccgaa cgtcgccggc ccaagtgatc cggtgcagcg
    18061 aaccatgtcc ctgcgacttc gcttgattgc caccgaaccg tcacatctat ggttcgcggt
    18121 tgccggacag ccgaccgcga tcatcgcggt gtcaaggccc gagaaccgcg tcaacgccca
    18181 gcatgtgccg cccgcggtgg tgcgtgcgat tgaagcagtg tacggccggc ggcccgttat
    18241 cggttggtcc ggcgaacgtg taccgatttc ccaagcggca caagcatttc gaaatgcccg
    18301 gttggcgttg accctggctg agatcggagt ctccgagaat aaggtagcgg tctggacgga
    18361 actaggctca tggagaacgc tcgcgattct cgcggacagc tactcaaaga accccaaaga
    18421 tctcgccgag ctagtgcatc cgggaatcgt cgacatggtc gaaggtggcc gcgacgacct
    18481 cgttcatacc ctcgacgcat atctcacaca tggctgcgat gcgcgaaaaa ccgcagagtc
    18541 gttgcacctt caccgttcga cgctgtatta ccggctcgag aagatcaccg aggcagtggg
    18601 aggtgatctg agggatggtg aggctcgatt cgagctgatg ctgagcatcc ggctcgcgaa
    18661 tattgcacgg ctgtaccaca tttaaacggc ttcgtcggac acatgcccaa gatcggcgcc
    18721 cgtccgacag ttgtccgaac acgccggcaa aagttccgac atcacgccgg tgacggtcaa
    18781 ctctcggcgc ctctactatc aacttccaaa tctagcccct gaagccacgc cgcggcctac
    18841 gcttggcgga tagtttgatc agacaaccgc tcgtattgac attgcgtctt caggtccacg
    18901 gcgatcgttc tgccgcactg agcatcggag ggatgccatg agcgcaacac ccacattgaa
    18961 gaaggcgctg agccagcggc agctgacgat gatcgccatc ggcggcgtga tcggtgccgg
    19021 cctattcgtc ggctcgggcg tcgtcatagg cgacaccggg ccagggtcgt tcatcacgta
    19081 cgccctggcc ggcgtgctga tcatcatggt catgcggatg ctggccgaga tggccgtggc
    19141 caacccgtcg acgggctcgt tcgccgacta ttcccgaaac gcgctgggca actgggcagg
    19201 cttctcggtc ggctggttgt actggtattt ctgggtgatc gtcgttggct tcgaggcgat
    19261 cgccggcgcg aagatcatcc aatactggat cgacgtcccg ttgtggttgt ccgccttgat
    19321 cctgatgatc ttgatgactg ggaccaacct attctcggtg tcgtccttcg gcgagttcga
    19381 gttctggttc gccggcatca aggtagccgc gatcgtggtc ttcatcgggc tgggctcgct
    19441 cttcgtgctc ggcttatggc caaacaagtc gctcgatttc tccaacctgt gggcccatgg
    19501 cgggttcttc ccgatgggtg tcatggcggt caccgtcggt gtggtcacgg tgatcttctc
    19561 catggtcggc gcggagatcg cgacgatcgc cgccgcggaa tcgtcggatc cggagagggc
    19621 ggtgtcaaaa gctgccaact cggtgatcac gcgtatcgca gtgtttttcg tcggctcgac
    19681 gttcctactc gcggtaatcc tgccgtggaa cgacgagaag gccggcgcgt cccccttcgt
    19741 ctccgcgttc accaagatgg ggatcccgta cgccgaccac atcatgaacg ccgtggtgtt
    19801 gaccgcggtg ctgagctgtc tcaactctgg catgtacacc gcatcccgca tgctgttcgt
    19861 gctcgccgcg cgccgggagg cgccggcgca gctggtccag gtgacccgtc gaggggtgcc
    19921 ggccatcgcg attctcgcgt cctcggtcgt cggcttcctg tgtgtcatcg ccgcggctgt
    19981 ctcgcccgac accatcttcg cgttcctgct gaactccagc ggcgcgatca tcctgttcgt
    20041 ctacctgctc atcgccgtct ctcagatcgt gctgcgatac cgcacccccg actcagagct
    20101 acgagtcaag atgtggctgt tcccggtgtt gtcgatcatc accgcggtcg cgatcgtggc
    20161 gatccttgtt cagatgggct tacaggcgga tgtgcggtct caactcgtgc tgagcctgct
    20221 gtcgtgggcc gtcgtcatcg tgctgttcct tctgaacaag tggtacgtga acagccggcc
    20281 cgtcgtcgag ggcgtcgcgc cgaccgagcg accgcaccgc gtgctcgtgc tggccaatca
    20341 gacggtcgga tctgcggagc tgctcgacga acttcgtcgc atcggcgcgg acaaaa
//
LOCUS       DASRJA010000040        20029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1096674,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000040 DASRJA010000000
VERSION     DASRJA010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20029
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096674"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1391..1867)
                     /locus_tag="VFC01_01815"
     CDS             complement(1391..1867)
                     /locus_tag="VFC01_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78412.1"
                     /translation="MTTHQRDIMAHLRIAPTGPLRIGVVLGDRVWLDTPGDVVRVRAA
                     DLRGVTQKAAALREERPNVHALVDIDVMIARDAHSARAAMAAAPPTDGTTLLYVGTPV
                     GLAGLIADIHALGIADGAVLIPLTRDGVMDLIRDEVLPEVRTMVSLHTETTACRTA"
     gene            complement(2055..2825)
                     /locus_tag="VFC01_01820"
     CDS             complement(2055..2825)
                     /locus_tag="VFC01_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZN78413.1"
                     /translation="MLNTIARTGTVLGLFTAETPEWGVTEVAATLGVPTSTTFDLLAS
                     LAEIGLLQQTMDDRYRLGWQLLVIRRRLINSMGVNDQTQRTVAELAQHLSAAVTIGAC
                     DGRGVVCIAHASVSRSGPVLAEGARLPGHVSALGKFLIAQFPWPKVRERIEQYGLPAL
                     TVNSVSDVDVFLKQLTCARENDLAVEHGETVIGQSCLAVGIYAREHRVLAALSICTPS
                     ETMHARHEEFARIARRAARTLLKQGISSHVPPVKYRNL"
     gene            complement(2883..3059)
                     /locus_tag="VFC01_01825"
     CDS             complement(2883..3059)
                     /locus_tag="VFC01_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78414.1"
                     /translation="MSTRCNEQLTFDIPPYSAVAAKCARPLSANGTQIIVAITESVVM
                     AQGNSEIADSHVRD"
     gene            3627..6635
                     /locus_tag="VFC01_01830"
     CDS             3627..6635
                     /locus_tag="VFC01_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN78415.1"
                     /translation="MMDNNPFRTQRDVGSAVVTELRAAGFDDADEIGRGGFGIVYRCT
                     QIALDRTVAVKVLTAELDENRERFLREQRAMGRLTGHPNIVSVLQVGETQSGYPYLVM
                     QYHRHGSLEARIGQLGPLPLDEVLRLGVKMAGALGTAHRLEILHRDVKPANILLTNYG
                     EPALGDFGIAHISGGFKTATGTFTGSPAFTAPELLSGDPPSRSSDVYGLGATLFCALT
                     GHAAFERRSGEQMVAQFLRIASESAPDLRDNGIPDDVAAVVERAMSRAPKDRLSAAGL
                     GEEIQGVQARYGFAVDEMALEAASGARRPEPRAAVAAGPSRNLGNLPLDLTSFVGRRT
                     ELAEVMELLSNARLVTLTGIGGVGKTRLALRAAADAAADFCDGVRLVELGELRDSSLL
                     VDVVAAGLGVRDASARPLIDVLVEFLRSRQMLLLLDNCEQVVEAAAKLTDAMLRACPQ
                     LRILATSREAIGIGGESVRQLSPLSFPDDSAPTPDGLSGYDAVALFAERAGSAVPGFC
                     LTDENKATVARICSRLDGLPLAIELAAARLRAMSPEQILERLADRYTLLTRGSRGAPA
                     RQQTLGWSIGWSYDLCAPAEQQLWGRLSVFAGSFELEAAEDICGSDLAPGDIDDLVSA
                     LVEKSILMRTETKGVVRFRILETLRAYGRERIQQTGTYTELRRRHRDWYRRLLLDAEA
                     EWFSSQQVHWIQRIEREMPNLREALEFSLSDNGETALGIVAGLQPIWISRGMLSEGRR
                     WLDPALGGIPPEPTADRIKALYGAATIAGIQGDVPAAEARVTEARALVQQMADPAARG
                     LTDVADAYTALLSGKFERTYQCCEDALNASDDPIVRAGSMLLMAWALEFEGDIGRALI
                     WGEKALAFAESHGESVYRTYALWNLGVGWWRQGQPDRGEQLLRQCLQLTQRVNDPRHA
                     AACLEALAWIAQAEQEPRRAVVLMGAADEIGRAVGASTAVFPNLVSFHEECERSAREA
                     LYEQAFETAYSEGGALSIDDAVAFALGDTA"
     gene            complement(6934..8364)
                     /locus_tag="VFC01_01835"
     CDS             complement(6934..8364)
                     /locus_tag="VFC01_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZN78416.1"
                     /translation="MNYLVTVLYIIAFAMFIYGLMGLTGPKTAVRGNWIAAAGMGLAV
                     IATLMAIRHTENWGLIIAALLLGVALGVPPARLTKMTAMPQLVALFNGVGGGTVALIA
                     LSEFIDTSGFSAFQHGESPTVHIVVASLFAAIIGSISFWGSIIAFGKLQEILPGRPIG
                     IGKAQQPFNLLLLAGAVVAAVAIGLAAHPGSGGASPWWMIGLLVAAGVLGLMVVLPIG
                     GADMPVVISLLNAMTGLSAAAAGLALNNTAMIVAGMIVGASGSILTNLMAKAMNRPIP
                     AIVAGGFGGGGAVGGGDGGDGGEHTAKSTSAADAAIQMACANQVIVVPGYGLAVAQAR
                     HAVKDMAAQLEKRGVPVKYAIHPVAGRMPGHMNVLLAEADVEHEAMKEMDDINDEFAR
                     IDVTLVIGANDVTNPAARNHPSSPIHGMPIRNVDKSRSVIALKWSMNYGFVGIDNPLF
                     YADGTTMLFGDAKKSVSEVTEELNPL"
     gene            complement(8361..8690)
                     /locus_tag="VFC01_01840"
     CDS             complement(8361..8690)
                     /locus_tag="VFC01_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZN78417.1"
                     /translation="MYDQLLANLAILVLSGFVGFSVISKVPNTLHTPLMSGTNAIHGI
                     VLIGALVVLGRVEHPTLAEQIILFVAVVFGTLNVVGGFVVTDRMLGMFKAKKTAAVKV
                     STNGQAR"
     gene            complement(8691..9764)
                     /locus_tag="VFC01_01845"
     CDS             complement(8691..9764)
                     /locus_tag="VFC01_01845"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019667174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HZN78418.1"
                     /translation="MTLIGVPRETAAGERRVALVPKVVERLRAAGVAVVVEPGAGTGA
                     LIPDEQFERAGAFIGDPWPADLVVKVNPPTTDEIGLLKPESTVVGFLAPRTRPEVTEA
                     LRLAGVTGFAMEAIPRISRAQTMDALSSQANVAGYKAVLVAASEATRFFPMLTTAAGT
                     VKPATVLVLGVGVAGLQALATAKRLGGKTTGYDVRPEVADQVRSVGAQWLDLGIDAAG
                     EGGYARELSDEERAQQQVALENAITGFDVVITTALVPGRPAPRLVTAAAVEGMKPGSV
                     VVDLAGETGGNCELTEPGQTVVRHDVTIASPLNLPSTMPEHASELYAKNIASLLDLLI
                     KDGEVAPDFDDEVVAASCVTREA"
     gene            9856..10863
                     /locus_tag="VFC01_01850"
     CDS             9856..10863
                     /locus_tag="VFC01_01850"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZN78419.1"
                     /translation="MRIAVVGAGAIGAYWGAALHRGGAEVHLIARNEHLRAMRENGVR
                     VLSARGDFVAYPNATDDPTEIGPVDYVFLGLKAHSYPSCGPLVEPLLGEHTAVLPAQN
                     GIPWWYFHQLTGPYQGRRIEAVDPGGATSAVLSPERAIGCVVYPATTIEAPGVIRHLE
                     GTRFSIGEPSGEISQRCKALSEAMIAGGLKAPVEPNLRDDIWIKLMGNVAFNPLSALT
                     RATMVEICQNPHTRQIVTQLMEETLDIAARVGSTPDISIEKRLRGAENVGHHKTSMLQ
                     DLEAGKQLELDAIVTAVVEMADLTGAAAPTLRTVHAATDLLARTSVPEAWHPEPQPAL
                     R"
     gene            10876..12294
                     /gene="pyk"
                     /locus_tag="VFC01_01855"
     CDS             10876..12294
                     /gene="pyk"
                     /locus_tag="VFC01_01855"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZN78420.1"
                     /translation="MNRRAKIVCTLGPATATAPQLTELIDAGMDVARLNFSHSTHAEH
                     SAVYDTVRRIAAERGRAIGVLADLQGPKIRLGRFAEGPVVWATGERIVITTAACSGDH
                     DRVSTTYDGLASDVRPGDRLLVNDGKIELQVLDVKSDDVHCEVIHGGPVSDNKGISLP
                     GVAVSVPPLSEKDIEDLKFALELGVDMVAMSFVRAPEEVKLAHAVMDEMGKRVPVMVK
                     LEKPEAVSDLQAIVDAFDGLMVARGDLGVEMPLEQVPLVQKRAITLCRQAAKPVIVAT
                     HMLDSMVADLRPTRAEVSDVANAVFDGTDAVMLSAETSVGAYPAHTVETMGRIVATAE
                     DALRQRKAPEAVLREDEDASISDAVAAAASELGRRIGAVALCCFTRSGDTAMRLARQR
                     SPLPLIAFAHNESVRARLAFSWGIQSAVLAPSSTPETMPAEVSRGLSDLGISRPGSLV
                     VVVSGSRSGVAGYTDRMRVLRI"
     gene            complement(12376..12834)
                     /locus_tag="VFC01_01860"
     CDS             complement(12376..12834)
                     /locus_tag="VFC01_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZN78421.1"
                     /translation="MTESGTMGVTEEWLAAAVVKESPEAIVVTDPAGLIRLWNDGAAR
                     MFGYSTEEAIGQNLDIIIPEKLRERHWKGYFQTMATGYTRYGDKLLSVPATHRDGQRL
                     SIEFSVALLRDDADQIVGISAVMREITERRNAEKALRTKITELENRAGPA"
     gene            12945..13877
                     /locus_tag="VFC01_01865"
     CDS             12945..13877
                     /locus_tag="VFC01_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN78422.1"
                     /translation="MLLRQLEYFVAVARERHFARAAESCYVSQPALSTSIAKLERELS
                     VTLINRGQNFEGLTPEGERLVVWAKRVLAEHDALKAEAAAMRSGISATLRLGTGPTVS
                     TTAALPVAAFCALHPLARVKVCSRLSSTELLRQLRDFELDAAIAHFGPDDQSGLEVVP
                     LYEEQYVLLVSGEQLVPAARTITWAEAAQLPLALLTPDMRFRQFIDKAFAESGAVAAP
                     QVETDSVASLYAHVATGAWASVVPHTWLRAMPVTGAARAIRLVEPDTRAQISMAIHAA
                     TPGSVAARAFLNVAVGTPLNDIFELHGKDLVHVA"
     gene            14027..15769
                     /gene="oxc"
                     /locus_tag="VFC01_01870"
     CDS             14027..15769
                     /gene="oxc"
                     /locus_tag="VFC01_01870"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HZN78423.1"
                     /translation="MTIASLPDTEVEHTPEPHEPGALTDGIHLVVDALKLNDVDTIYG
                     VVGIPITDLARLAQASGIRYIGFRHESDAGHAAAAAGFLTQKPGICLTVSAPGFLNGL
                     VALANATTNCFPMVQISGSSERHLVDLQRGDYEEMDQLAAARLFAKAAYRVSRAEDIG
                     RGIARAIRTAVSGRPGGVYLDIPAAVLGEVIDAEKAAATLWRVVNPAPRQLPETEMVD
                     SAIDLLANAKRPLIVLGKGAAYAQADEQIREFVETTGVPYLPMSMAKGLLPDDHPQSA
                     ATARSLALKRADVVMLVGARLNWLLGHGDAPQWNPDAKFIQVDIAAAEMDSNQPIAAP
                     LVGDIGSVMEALLERAKPGQITVQTEWREELTAKSDSNVAKMAGRLEAALTAHPMKFL
                     GALQAIRDVLHDNPQVYVVNEGANALDLARNTIGMQVPRHRLDSGTWGVMGIGMGYAI
                     AAAVETGDPVVAIEGDSAFGFSGMELEAVCRYNLPIVTVILNNSGVYRGDDASTTSDP
                     APTALRARHEYMIKAFGGKGYQATTPAEVGAALREALASGRPALIDCVIDPSDGTESG
                     NIAHLNPKGITAKH"
     gene            15817..17073
                     /gene="frc"
                     /locus_tag="VFC01_01875"
     CDS             15817..17073
                     /gene="frc"
                     /locus_tag="VFC01_01875"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HZN78424.1"
                     /translation="MTDLPLSGIKVIDFTGVQAGPACTQMLAWFGADVLKVERTSGGD
                     VTRNQLRDIPELDALYFTMLNSNKRSLAINTKSPEGLEVMEKLIRGADVLVENFAPGA
                     MDRMGLSWDKIQEWNPRLIFGSVKGFNDDSSWNDLKVYENVAQCAGGNASTTGFWDGP
                     PTVAGAALGDSNTGMHLLIGILTAIIQRDKTGKGQKVSAAMQDAVLNLCRVKLRDQQR
                     LEKVGYLEEYPQWPNGEFGDAVPRGGNAGGGGQPGWVVKCKGWETDPNAYIYFTIQEQ
                     NWKRTAEAIGRPEWVEDPGYNTARARQDKIFDIFAEIEKWLADKTKWEAVDILREWEV
                     PCAPVMSMKDLAEDKDLRKSGSIVEVEQKGRGTYLTVGSPIKFSEFKPDIKGAPLLGE
                     HTDEVLAELGYDADTIAAWHEKKIVV"
     gene            17150..17608
                     /locus_tag="VFC01_01880"
     CDS             17150..17608
                     /locus_tag="VFC01_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HZN78425.1"
                     /translation="MSMTLVREIAASHRGQRGALLPILHAVQEALGCVPPEAIPVLAD
                     ELNLSRADVHGVVSFYHDFRSAPAGRTTVRICRGEACQAVGAERLVSQLQDRCGMSLG
                     ETSRDGSLTVEQVFCLGNCALGPAAQVNGRLRGRLDEARLSAIVDEAVVQ"
     gene            17605..19176
                     /locus_tag="VFC01_01885"
     CDS             17605..19176
                     /locus_tag="VFC01_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZN78426.1"
                     /translation="MSAPVTVYVPSDSAARSVGADEVADALASAAARQGRSIRLVRNG
                     SRGLLWLEPLVEVETEDGRIGYGPVAASDVDGLVSGGLFDGAELPQRLGVVDELPWLA
                     AQNRVTFARMGVTDPLSPDDYLRHGGMVGLVRALERSPEDVVAEVTDSGLRGRGGAGF
                     PAGIKWKTVHDCVDALKFVCCNADEGDSGTFADRMLMEGDPFLLIEGMTIAAYAVGAT
                     EGYVYIRSEYPEAVATMRAAIEIAYGRGWLGDNVLGSSLNFDLQVRVGGGAYICGEET
                     SMLESLEGKRGMVRAKPPIPAINGLFGKPTVVNNVLTLATVPTVLAHGAQAYQQLGVE
                     RSRGTQVFQLGGNIKHGGIVETAFGITLGELVDGYGGGTRSGRPVRAVQVGGPLGAYL
                     PRAKFDLPMDYEAFVAAGAMVGHGGIVVFDETVDMAAQARFAMEFCAAESCGKCTPCR
                     VGAVRGVEVIDRITAGEHRDENLALLEDLCDVMTEGSLCAMGGLTPMPVRSAIEHFPD
                     DFYARHASAKTEGKP"
     gene            19173..>20029
                     /locus_tag="VFC01_01890"
     CDS             19173..>20029
                     /locus_tag="VFC01_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZN78427.1"
                     /translation="MTLLKEPDFGTPAKDGPASVSLEVDGMRVSVPEGTSVMRAAAMA
                     GVDVPKLCATDRLEAFGSCRLCLVEIDGRRGTPASCTTPVADGMVVHTQTPKVAKLRE
                     NVMELYISDHPLDCLTCAANGDCELQDMAGAVGLRQVRYGYEGENHLDAVKDQSNPYF
                     DFDPSKCIACSRCVRACDEIQGTFALTIEGRGFDSKVSPGAGESFMDSECVSCGACVQ
                     ACPTATLQEKSVIQLGMPSRSVITTCAYCGVGCSFKAELRGDEVVRMVPHKDGGANEG
                     HSCVKGRFA"
BASE COUNT         3531 a         6358 c         6675 g         3465 t
ORIGIN      
        1 cgccgacgcc gtcggcgccc cccgtgcgat cggccgacat accacaccgc gcagtgcaca
       61 accggcgtca atgcggttag gagataagac gctaggtcgc cgggtctgca ttgacatcac
      121 caacgttcgt cgcgcacggc agttgatcag cccaagtacc tccgtgcaag aacggctcgt
      181 tcgcaacgca actgggggtc gcttggctgc ggcggacatg acgacacgac gattgcgggg
      241 ttcgttgtcc ggttgttcga agagcggttt gctgggcagc catggtggct gatgtccgct
      301 gtccgtgcca ttggcgcggc aatattgatg agtgcgggac gtcgtgaaca tctggtgtgc
      361 cgactggatg gatgatctcg caatatccac gtctcctcgt gtacgaagcc cgtttgtgag
      421 ctaacccact atggatggaa aaagtgacgt ggatctgttc tgatcgcgaa cattcacagg
      481 gcgccgaggt gctgacgagt cgcctgggac cggtccaaac actgaacgcc aattgtgagg
      541 cgatgcgttg ccgccgcggt gacactcgcc cgggcggcac gatggtgaca cggttgggtc
      601 ggcgctttac gcggccttga atgcctcggc cagggcgttc gcagctatcg ccgcgggacc
      661 cgaccgacgg ggcgatcccg agctcgccga tgcggtcggt agaggccagc gaaagttact
      721 aggatccgcg gtcggactga tggaccaact ggggttgaat cagaatcaga ttgccacaca
      781 gcgtgattga acgcccgcgg gctcaggtct gctgctggcc gctaccgtcc agccgacagc
      841 gcctcgatgc acgaacgcgg tatagcaaga ccccgcacgg gcccgcacgt aggtgatgtt
      901 ggctacctac agtcggttcg ggccgcggct gcgaagcgtc gctgcatcga ctgtcctttc
      961 ggcgtccgcg gtcagacata ccttcgatcg aaagattctg tatcaggccg aggcttaaag
     1021 gaacccgcgg atgacatctc gtgcgaagtt gctcggatgg tgaccgtcga acatattcgc
     1081 gttcatgcgc cgacaatgat tgggagagag ctgaactgac gaagttcacg tcggaaccgg
     1141 gcgggtgatc gtcaagggct cgggaagtgc ggcgcgatcg tcgacaggag ttcgaacccc
     1201 ctctcatgga tcgctaggtc aagcgagctg tctgcgctca gcacattcgc cgttagaggt
     1261 gggcgccgtc acaatcgcgc aacggcggcg acgcgtcgac cgtgtcggca cgatggatgt
     1321 cccggacgag tcggagtacg accggcccga tgccggggca gcgccgcacg gtagtcctcg
     1381 gctgtgatcg tcacgcggtg cggcacgccg tggtctcggt gtgcagcgac accatggtgc
     1441 gcacctcggg cagcacctcg tcgcggatca gatccatgac gccgtctctt gtgagcggga
     1501 tcaatacagc gccatcagcg atgccgaggg cgtgaatgtc tgcgatcaga ccggcgagac
     1561 cgacgggtgt gccgacgtag agaagggtgg tgccgtccgt gggcggcgcc gccgccatcg
     1621 ctgcgcgcgc cgagtgggcg tcgcgggcga tcatcacgtc gatgtcgacc agcgcatgca
     1681 cgttcggccg ctcctcccga agcgcggcgg ccttctgggt gacgccacgc agatcggcgg
     1741 cgcggacgcg gaccacgtct ccaggggtgt ccagccacac gcgatcgccg agcacgacgc
     1801 cgatgcgcag aggcccggtc ggtgcgatac ggagatgcgc catgatgtcc ctctgatgtg
     1861 tggtcactaa gatcgcttcc cctcgtaacg cggataggct cctcgcattc ccgcgaagga
     1921 cgggcagggt tgtgcaaggg tcacttttcc tcgtccatcg tgcgctgagc tccagagatg
     1981 atggcgcttg gtcgtcgctg ccgcgaggcg tctgaccgcg cccgtcgacg gaggtgtgga
     2041 actcctcgta gtcatcagag attccggtat ttgaccgggg gtacgtgcga tgagatgcct
     2101 tgcttcagca gggtgcgcgc agccctgcgg gcgatcctgg cgaactcttc gtggcgggca
     2161 tgcatggtct cggacggcgt gcagatcgac aacgcagcaa gcactcggtg ctcgcgggcg
     2221 tagatgccca cggcaagaca cgactgaccg atgacagttt ccccatgctc cacggccaga
     2281 tcgttctccc gcgcgcaggt cagctgcttg agaaagacat cgacgtcgga taccgagttc
     2341 accgtcaacg cgggcaagcc gtactgctcg attcgttctc gcaccttcgg ccaggggaac
     2401 tgagcgatga gaaacttccc aagagccgac acgtgacctg gcaggcgagc accctcggcc
     2461 aggactggtc cgctccggga caccgacgcg tgcgcaatgc agacgacccc ccggccgtcg
     2521 caggccccga tggtgacggc cgcgctcagg tgctgggcga gctcggcgac ggtgcgttgc
     2581 gtttgatcat tgacgcccat ggagttgatc agccgacgtc ggatcacaag gagctgccac
     2641 ccgagacgat atcggtcatc catagtttgc tggagcaaac caatctcggc gagactggcc
     2701 aataggtcga aggtggtcga cgtgggcacg cccagggtcg ccgcgacctc ggtgacgccc
     2761 cactcgggcg tctcagcagt aaacaggccg agcaccgttc cggttcgagc gatcgtgttc
     2821 agcaagtcgc cagccttcca gaacggtcca gagcggactc cacggtgacc agatccgttg
     2881 tgtcaatctc gtacatgcga gtcggcgatt tcgctgttgc cctgcgccat cacgacgctc
     2941 tcggtaatcg cgacgatgat ctgggtgccg ttagccgaga gcggccgcgc gcactttgcc
     3001 gccaccgccg agtagggcgg aatgtcgaag gtcagctgct cattgcacct tgtcgacacc
     3061 ggcgctcctc tccgcgggtc agcagaccac cggctgacat atcaggacgc tggtgatata
     3121 ttaaacacga tgttggccta ggtcaagcaa tgccagagga gttccctgcc cgctcgcgcg
     3181 ctcctcgagg cggccctcaa ccagaccgac aatgcccagc catcgcggaa actcgtgagg
     3241 tggccggccg ggatcatcct tccgctgaac ggcgtcgatc gagagcacct gtgagcgagt
     3301 cgggcggtgt aacgctggtg tgcgcgccgg caggcagcga caagacggtg ttgctgcggg
     3361 cctgggttga ccacgccaac ctggatgatc ccaccggatg ggtgtaccag caggcgaatc
     3421 ctgaggagac aggtccaccg ggtcgagtgg cagcatcgct agcgtatgac gccaaacatc
     3481 ggtcatgtct acatacttgg ccgggcggtt gtgcgatgaa atcgctgcgc cgaggtaatg
     3541 cgtgacatcg caatcgacaa cgcactgccc gaagtccacc tggggtttgc tccgaggcct
     3601 acgctcagat tcatcgaagg aactcgatga tggacaacaa tcccttcagg acacagcgcg
     3661 acgtgggctc ggcggtcgtc acggagttgc gcgcggctgg attcgacgac gccgatgaga
     3721 tcggacgcgg tggcttcggc atcgtctatc gctgcaccca gatcgccctg gaccgcaccg
     3781 tggcggtgaa ggtgctcacc gcggaacttg acgagaatcg ggagcggttc ctgcgcgagc
     3841 agcgtgccat gggtcggctc accggacatc cgaacattgt cagcgtgctg caggtcgggg
     3901 aaacccagag cggctacccg tacctggtga tgcagtacca ccgccacggt tcgctcgagg
     3961 cgcgaatcgg ccaactcggt cccctaccgt tagatgaggt gctgcggctc ggggtgaaga
     4021 tggcgggcgc cctggggacg gctcaccggc tcgagatcct gcaccgagac gtcaagcctg
     4081 cgaatatctt gctcactaac tacggcgaac ccgcattggg cgacttcgga atcgcccaca
     4141 tctcaggcgg attcaagacg gcgaccggta cctttaccgg ctccccggcc ttcaccgcgc
     4201 ccgagctcct cagcggtgat cctcccagcc ggtcctctga tgtgtacggg ctcggcgcca
     4261 cgctgttctg cgcgctcacc gggcatgccg ctttcgagcg ccgaagcggt gagcagatgg
     4321 tggcgcagtt cctgcgcatc gccagcgagt cggcgccaga cctgcgcgat aacggcatcc
     4381 cggacgacgt tgccgccgtg gttgagaggg caatgtcccg cgccccaaag gaccgcctgt
     4441 ccgctgcggg gttgggtgaa gaaatccagg gggtacaggc ccgttacggg tttgcggtcg
     4501 acgagatggc gctggaggcc gcctcggggg cccgccgacc cgagccccgt gcggcggtgg
     4561 cggctggtcc ttctcgcaac ctgggcaacc ttccgttgga tctcaccagc ttcgtcggtc
     4621 gccgaactga gctggcggag gtgatggagc tcctgtcgaa cgcacgtctg gtgacgttga
     4681 ctgggatcgg cggggtcggc aaaacccgac ttgcgttgcg agccgccgcg gatgcggccg
     4741 cggatttctg cgacggtgtg cgtctggtcg agttgggtga gctgcgcgac tcttcgttgt
     4801 tggtcgacgt cgtggccgcc ggattgggcg tgcgtgacgc gtcggccaga ccgctgattg
     4861 acgtattagt cgaatttctg cgctcgcggc agatgctgct gctgcttgac aactgcgagc
     4921 aggtggtcga ggcagcggcg aagctgaccg acgccatgct gagggcgtgc ccgcagttgc
     4981 ggatcctcgc gaccagccgc gaggccatcg gcattggcgg cgagtcggtg cggcaactct
     5041 cgcccctctc gttccccgac gactccgccc ccacgcccga cgggctatcc ggttacgacg
     5101 ccgtcgcgtt gtttgccgag cgggccggct ccgcggtacc aggcttttgt ctcaccgacg
     5161 agaacaaggc gaccgtggcg cgcatctgct cgcggctgga cggattgccg ttggcgatcg
     5221 aactcgccgc ggcgcgactt cgcgcgatgt cccccgaaca gatcctggag cggctggccg
     5281 atcggtacac gctgctgacc cgtggcagcc gcggtgcacc ggcgcggcag cagacgctcg
     5341 gctggagcat cggttggagc tatgacctgt gcgcaccggc tgagcagcag ctgtggggcc
     5401 ggctttcggt gttcgccgga agtttcgaac tcgaagcagc agaagacatt tgtggcagcg
     5461 acctggcccc cggcgatatt gacgatttgg tgtccgcgct cgtggagaag tcgattctga
     5521 tgcgaacgga aaccaagggc gtggtgcggt tccggattct tgagacgctg cgcgcatacg
     5581 gccgggagcg catccagcag accgggacat acacagaact gcgccggcgg caccgcgatt
     5641 ggtaccggcg actgctgctc gacgccgagg cggagtggtt cagttcgcag caggtccatt
     5701 ggatccagcg catcgagcgg gaaatgccca atctgcgcga agccctggag ttcagcctct
     5761 ccgacaacgg cgagaccgcg ctcggcatcg tcgccgggtt gcagccgatt tggatctccc
     5821 gcggaatgct cagcgagggt cgccgctggc tggaccctgc gctgggtggt ataccgcccg
     5881 aacctactgc cgaccggatc aaggcgcttt acggcgcggc gacgatcgcc ggcattcagg
     5941 gtgacgtgcc ggcggcggag gcgcgagtga ctgaggcgcg ggccctggtc cagcagatgg
     6001 ccgaccctgc agcacgcggc ctgactgacg tcgccgacgc atacacggca ctgttaagcg
     6061 gcaagttcga gcgcacctat caatgctgcg aggatgccct taacgccagc gacgatccga
     6121 tcgtgcgggc cgggtccatg ctgctgatgg cctgggctct cgagtttgag ggcgatatcg
     6181 gccgggcgct catctgggga gagaaagcac ttgccttcgc agaatcccac ggcgagtcgg
     6241 tatatcggac gtatgcgttg tggaacctgg gggttggctg gtggcggcag ggtcagcctg
     6301 accgcggcga gcaactgctg agacagtgtc tccagctgac gcagcgggtc aacgatcctc
     6361 gccatgccgc ggcgtgtctg gaggcgttgg cctggattgc ccaggcagag caagaaccac
     6421 ggcgtgccgt ggtgttgatg ggagcggccg acgaaatagg ccgtgccgtt ggggcttcga
     6481 cggcggtctt tcccaatctg gttagcttcc atgaagagtg cgaacgcagc gctcgggaag
     6541 cgctttacga gcaagccttc gaaaccgcct acagcgaggg cggcgcactg agcattgacg
     6601 atgctgtggc ctttgccctc ggtgacaccg cttaggcccg atcggcacgg aacgggcttt
     6661 cgactacaag cacgcggcca gaccagccct gcaccacgac cagcgcgcag gtggtggcta
     6721 gcggaccgtg tggagcgtga tctcatcaga gtcctcgccg acgccgcgaa gtggctcgac
     6781 acgaacgaga ccttcggctg gtgctcaagg ctgacacgag cggccactga ggccagatca
     6841 tggtgtcgat cgccgtctgg atcgcgctgc accgacagcc gtaaaagccc tccgtttggc
     6901 gtgtcggggt tttgcgtctc agtgtcagtt ccgctatagc ggattgagtt cctcggtgac
     6961 ctcggagacc gatttcttgg cgtcgccgaa gagcatggtg gtgccgtcgg cgtagaacag
     7021 tgggttgtcg ataccgacga agccgtagtt cattgaccac ttgagtgcga tcaccgagcg
     7081 ggatttgtcg acgttgcgga ttggcatgcc gtgaatgggt gagctcgggt ggttgcgtgc
     7141 ggcggggttg gtgacgtcgt tggcaccgat gacgagggtg acgtcgatgc gggcgaattc
     7201 gtcgttgatg tcgtccattt ccttcatcgc ctcgtgttcg acgtcggcct cggccagtag
     7261 cacgttcatg tgcccgggca tccggccggc caccgggtgg atcgcatact tgaccggcac
     7321 cccgcgtttc tcgagctgcg cggccatgtc tttgaccgcg tgccgggctt gggcgacggc
     7381 cagcccgtaa cccgggacga cgatgacctg attggcgcag gccatttgga tcgcggcatc
     7441 agcggccgag gtggacttcg ccgtgtgctc gccgccatca ccgccatcac cgccacccac
     7501 agcaccgccg ccgccgaagc cgccggcaac gatcgcgggg atgggccggt tcatcgcctt
     7561 ggccatcagg ttggtcagga tcgaaccgga cgcgccgacg atcatgccgg cgacgatcat
     7621 cgcggtgttg ttcagcgcca atcccgctgc ggcagcggac aatccggtca tcgcgttcag
     7681 caacgagatg acgactggca tatcggcgcc gccgatcggc agcaccacca tcaggcccaa
     7741 cacaccggct gccaccagta gaccgatcat ccaccacggc gacgccccgc cgctgccagg
     7801 atgcgcggcc agcccgatcg ccactgcggc gacgaccgca ccggcgagca gcaacagatt
     7861 gaacggctgc tgcgccttgc cgatgccgat cgggcggccg ggcaggatct cctgcaactt
     7921 gccgaacgcg atgatcgaac cccagaacga gatcgacccg atgatcgcgg cgaacagcga
     7981 cgccaccacg atgtgcacgg tcggcgactc gccgtgctgg aaggccgaaa agccgctggt
     8041 gtcaatgaat tccgacagcg cgatcagcgc gacggtgccg ccacccacgc cgttgaacaa
     8101 cgcgaccagc tgcggcatcg cggtcatctt ggtcaaccgt gctggaggca cgcccagcgc
     8161 cacaccgagc agcagggcgg cgatgatcag gccccagttc tcggtgtggc ggatcgccat
     8221 cagtgtcgcg atgaccgcga ggcccatgcc cgccgcggcg atccagttgc cgcgcaccgc
     8281 ggtcttggga ccggtcagac ccatcaagcc atagatgaac atcgcaaatg cgatgatgta
     8341 cagcaccgtg acgagataat tcatcgcgct tgcccgttcg tcgaaacctt gaccgccgca
     8401 gtctttttgg ccttgaacat gcccagcatc cggtcggtca ccacgaatcc gccgaccacg
     8461 ttgagtgttc cgaacaccac cgcgacaaac aggatgatct gctcagcgag cgtggggtgc
     8521 tcaacacggc ccagcaccac cagcgcaccg atcaacacga tgccgtgaat ggcgttggtg
     8581 cccgacatca atggcgtgtg caaggtgttg ggcaccttgg aaatgaccga gaacccgacg
     8641 aacccggaca gcaccaggat cgcgagattg gccaataact ggtcgtacat ctatgcctcc
     8701 cgcgtgacgc atgaagcagc cacgacttcg tcgtcgaaat ccggtgccac ttcgccgtcc
     8761 ttgatcagca ggtccagcag cgacgcaatg ttcttggcgt acaactcact cgcgtgctcg
     8821 ggcatcgtcg acggcaggtt cagcggcgac gcgatcgtca cgtcgtgtcg gacaacggtt
     8881 tgtcccggtt cggtcagctc gcagttcccg ccggtctcac cggcaagatc gacgaccacg
     8941 ctgccgggct tcatgccctc cacggcggcc gccgtcacca atcgcggcgc gggccgcccc
     9001 ggtactaacg ccgtcgtgat caccacgtca aacccggtga tcgcgttttc cagcgctacc
     9061 tgctgctggg cacgctcctc atcggacagc tcacgggcat agccgccctc gccggccgcg
     9121 tcgataccca agtccagcca ctgcgcgccc accgagcgca cctgatcggc cacctcggga
     9181 cgcacgtcgt acccggttgt tttaccgccg agccgcttgg ccgtcgcgag tgcctgcagc
     9241 ccggcaacac ccacgccgag caccaggacg gtggccggct tcaccgtgcc cgccgcggtg
     9301 gtcagcatcg ggaagaatcg ggtggcctcg gacgctgcca ccagcaccgc tttgtacccg
     9361 gcgacgttgg cctgcgacga cagcgcatcc atggtctgcg cgcgcgaaat gcgcggaatc
     9421 gcctccatcg cgaagccggt gacgcccgcc aggcgcaggg cttcggtgac ctcgggacga
     9481 gtccgtggcg cgaggaaccc gacgacggtg gattcgggct tcagcagacc gatttcgtcg
     9541 gtggtcggcg ggttcacctt caccacgagg tcggccggcc acggatcgcc gatgaacgca
     9601 ccggcccgtt cgaattgttc gtcggggatc aatgcgcctg tgccagcgcc gggttcgacg
     9661 acgacggcca caccggcggc acgcagtcgt tcgacaacct tggggaccag cgcgacacgg
     9721 cgttctcctg ccgcggtctc tcgcggcact ccgatcagcg tcatcttcct cttcctggct
     9781 gtggccgggg accattgcca cagtagatga gatcgcatac tgtatgcaac cagtcccgag
     9841 agtcgaggag tcgccgtgag aattgcagtc gtcggtgccg gagcaatcgg cgcctactgg
     9901 ggtgccgcgc tgcaccgagg tggtgccgag gtccatttga tcgcgcgcaa tgagcacttg
     9961 cgggcgatgc gggaaaacgg tgtgcgggtg ctcagcgcgc gcggcgactt cgtggcctac
    10021 cccaacgcca ccgacgaccc caccgagatc gggcccgttg actatgtgtt cctcggcctc
    10081 aaagcgcaca gctatccgtc gtgcggtccg ctggtggaac cgttgctcgg cgagcacacc
    10141 gcggtgctgc ccgcgcagaa tgggatcccg tggtggtact tccaccagct gaccggccca
    10201 taccagggtc ggcggatcga ggccgttgat cccggcggtg cgaccagcgc cgtgctgtca
    10261 ccggagcggg caattggctg cgtcgtctat cccgcgacga caatcgaggc gccaggagtc
    10321 atccggcact tggagggcac ccggttctcc atcggcgagc cgtccgggga gatctcgcaa
    10381 cgctgtaaag cgttgagcga ggcgatgatt gccggtggtc tgaaggcgcc ggtcgagccg
    10441 aacctgcgcg acgacatctg gatcaagctg atgggcaacg tcgcgttcaa tcccctcagt
    10501 gcgctcacca gggcgacgat ggtcgagatc tgccagaacc cgcacacccg ccagatcgtg
    10561 actcaattaa tggaggagac gctcgacatc gcggcgagag tcggcagtac tccggacatt
    10621 tcgatcgaga agcggctgcg cggagcggag aacgtcggac accacaagac gtcgatgctg
    10681 caggacctcg aagcgggcaa gcagctggag ctcgacgcca tcgtcactgc ggtggtggag
    10741 atggcggacc tcaccggcgc cgccgctccg acgcttcgca ccgtgcacgc ggccaccgac
    10801 ctgctcgcgc gcacgtccgt gccggaagcg tggcaccccg aaccccagcc cgctctgcgc
    10861 tgaaggagga acaccgtgaa ccgacgcgcc aagatcgtct gcacgctagg acccgccact
    10921 gccaccgctc cgcaactgac tgaactcatc gatgccggaa tggatgtggc gcgactcaac
    10981 ttcagccaca gcacacacgc cgagcattcg gctgtctacg acacggtccg ccggatcgcc
    11041 gccgagcgcg gtcgtgcgat cggcgtgctc gccgacctgc agggaccgaa gatccggttg
    11101 ggtcgcttcg ccgaggggcc ggtggtctgg gccaccggtg agcggatcgt aatcacgacg
    11161 gctgcatgtt ccggcgacca tgacagggtc tcgacgacat acgacggcct ggcttctgat
    11221 gtgcgaccgg gggaccggct gctcgtcaac gacggcaaga tcgaactgca agtactcgac
    11281 gtgaaaagcg atgacgtcca ttgtgaggtc atccacggcg ggcccgtcag cgacaacaaa
    11341 ggcatctcgc tgccgggtgt cgcggtcagc gttccgccgt tgtccgagaa ggacatcgag
    11401 gacctgaagt tcgctcttga actcggtgtc gacatggttg cgatgtcctt cgttcgcgcg
    11461 cccgaggagg tcaagctcgc gcacgcggtg atggacgaga tgggcaagcg agttcccgtc
    11521 atggtgaaac ttgagaagcc ggaagcggtt tcggatctgc aagcgatcgt ggacgcgttc
    11581 gacggcttga tggtcgcccg cggcgacctc ggtgtggaga tgccgttgga acaggttccg
    11641 ctggttcaga agcgggccat caccctgtgc cggcaagcgg ccaagccggt gatcgtggca
    11701 acccacatgc tcgactcgat ggtggccgac ctgcggccga caagggccga agtctccgac
    11761 gtggccaacg cggtcttcga cggaacggat gccgtgatgc tctccgccga aaccagcgtc
    11821 ggcgcctatc ccgcccacac ggtggagaca atgggccgca tcgttgccac agccgaagat
    11881 gcactgcgtc aacggaaagc gccggaagct gtcctccggg aagatgagga tgcgagcatc
    11941 agcgatgccg ttgcggcggc cgctagcgaa ctcggcagac gaatcggggc ggtggcgctg
    12001 tgctgtttca cccgctccgg ggacacggcc atgcgcttgg ctcggcaacg atcgccgctg
    12061 ccgctgatcg ccttcgcaca caacgagtcc gtcagagcgc ggttggcatt ctcctggggg
    12121 atccagtccg ccgtgctggc gccgtcaagt acgccggaga cgatgcccgc cgaggtgagc
    12181 cgcggcctgt ccgacttggg tatctcgcgt ccgggcagcc tggtcgtcgt ggtgtccgga
    12241 agccgcagcg gcgtggccgg ttacaccgac cggatgcgtg tgctgcgcat ctaatgacgg
    12301 ccgaacgttc cttaccgctc gatatttcgc ccgattttcg aacggtaagg aacgttcgtc
    12361 gctatcgggg gccggtcagg ccggccccgc gcggttctcg agttcggtga tcttggtgcg
    12421 caaggctttc tcggcgttgc ggcgttcggt gatctcacgc atgaccgccg agatcccaac
    12481 tatctggtcg gcgtcgtcgc gtagcagcgc cacactgaac tcgatcgaca gccgctgccc
    12541 gtcgcggtgg gtcgcgggca cgctcagaag tttgtcgccg tagcgggtgt agccggtggc
    12601 catcgtctgg aagtagccct tccagtgccg ctcccgcagc ttctccggga tgatgatgtc
    12661 caggttctgg cctatcgctt cctcggtcga gtagccgaac atgcgggcgg ccccgtcgtt
    12721 ccacagtcgg atgaggccgg cggggtcggt caccacgatc gcctcgggag attccttcac
    12781 gaccgccgcg gcgagccatt cctcggtcac acccatcgtg ccgctttccg tcacgccaca
    12841 ctccgttcct cgttgatcgg tcgctggaca tcttcgcagc ggacctctag catacgcaat
    12901 atcgaatact gaatacaatc tggaaactta tcgaggaggg acctgtgctg cttcgccagc
    12961 tggagtactt cgtggcggtg gccagggaac gacacttcgc ccgtgcggcg gagagctgct
    13021 atgtgtcgca gcctgcgctg tcgacctcga tcgccaagct ggaacgcgag ctgagcgtca
    13081 ccctcatcaa tcgcgggcag aacttcgagg gtctgacgcc agagggcgaa cggctcgtcg
    13141 tctgggccaa gcgtgtcctc gccgagcatg acgcgctcaa ggccgaagcg gctgccatgc
    13201 ggtccggaat ctccgcaaca ctgcggttgg ggactggtcc cacggtgtcg acgaccgcgg
    13261 cacttccggt ggccgcattc tgcgcactgc atccactggc cagggtcaag gtctgctcac
    13321 ggctgtcatc tacggagttg ctacgccaac tgcgtgactt cgaattggac gccgccattg
    13381 cgcatttcgg cccggatgac cagtcgggcc tcgaggtggt gccgctctac gaggaacagt
    13441 atgtgctgtt ggtgtcgggc gagcagctcg tgcccgcagc acggaccatc acttgggccg
    13501 aggccgcaca gctgccgctg gccctgctga cccccgatat gcgctttcgc caattcatcg
    13561 acaaggcgtt cgccgaaagt ggcgcagtgg cggcacccca agtggaaacc gattccgtgg
    13621 catcgcttta tgcacacgtg gccacgggcg catgggcgag cgtggtgccg catacctggt
    13681 tgcgtgcgat gccggtgacg ggagcggcgc gagccatccg cctcgtcgag ccggacacca
    13741 gggcacagat ctcgatggcg atccacgcgg cgacacccgg ttcggttgcg gctcgcgcgt
    13801 tcctgaacgt ggcggtggga acgccgctga acgacatctt cgaactccac ggaaaggacc
    13861 tcgtccacgt cgcttgatgc gcgcagcgga cgcaacgtcg ctttggcccg tcgtgataac
    13921 cgccgtttat caggcaattg cagacaagtc ttggacgcgc gtcgtgacga cgcggaaagt
    13981 taactccaac aagggaataa gcgcgaacag ccaggaaggg cacaccatga ccattgcttc
    14041 cctgccggat acggaggtag agcacacccc cgagccgcac gagccgggtg cgctcaccga
    14101 tgggatccat ctggttgtcg acgcgctcaa gctcaacgac gtggacacca tctacggggt
    14161 cgtcgggatc ccgatcaccg atctggctcg tctcgcgcag gcatcgggca tccgctacat
    14221 cgggttccgt cacgagagcg acgcaggcca cgctgcggcc gcggccggct tcctcaccca
    14281 gaagcccggc atctgcctga cggtgtcggc tccgggcttc ctgaacgggc ttgtggcgct
    14341 tgccaatgcc accaccaact gctttccgat ggttcagatc tccgggtcca gcgagcggca
    14401 cctggtcgac ctgcagcgcg gtgactacga ggagatggat cagctggcgg cggccaggct
    14461 gttcgccaag gcggcctacc gagtatcgcg ggccgaggac atcggccgcg gcatcgcccg
    14521 cgcgatccgc accgcggtgt ccggtcgtcc cggtggcgtg tacctcgaca ttccggccgc
    14581 ggtgcttggt gaggtgatcg acgcggagaa ggcggcggct acgttgtggc gggtcgtcaa
    14641 tcccgcgcca cgtcagctgc cggagacgga gatggtcgat tccgcaatcg atctgctcgc
    14701 gaacgcgaag cggccgctga tcgtgctcgg caagggcgcg gcctacgcgc aggccgatga
    14761 gcagatccgc gagttcgtcg agacgaccgg ggtgccgtac ctgccgatgt ccatggcgaa
    14821 ggggctgctg cccgacgacc acccgcagtc tgcggccacc gcccgatcgc tggccctgaa
    14881 gcgggccgat gtggtgatgc tcgtgggggc tcgcctgaac tggctgctcg gccatggcga
    14941 tgccccgcag tggaatcccg acgccaagtt catccaagta gacatcgcgg ccgccgagat
    15001 ggacagcaat cagcccatcg cggccccgct cgtcggtgac atcggctcag tgatggaagc
    15061 cctcctggaa cgggcgaagc caggtcagat cactgtgcag accgagtggc gcgaagagct
    15121 cacggccaag tccgactcga acgtcgccaa gatggccggc cgcctcgagg ccgcactgac
    15181 cgcacacccg atgaagttcc tcggcgcgct gcaggccatc cgcgatgtgc tgcacgacaa
    15241 cccacaggtt tacgtcgtca acgagggcgc caacgcactc gacctggcgc gcaacaccat
    15301 tggcatgcag gtgcctcggc atcggttgga cagcggcacg tggggtgtga tgggcatcgg
    15361 catgggctac gcgatcgccg ccgccgtcga gaccggcgac ccggtcgtcg cgatcgaagg
    15421 cgacagcgca ttcgggttca gcggaatgga gctggaggcg gtctgccgct acaacctgcc
    15481 gattgtcacc gtcatcctca acaacagcgg tgtgtaccgc ggtgacgacg cctcaacgac
    15541 gtccgatccg gcgccgacgg cgctgcgagc ccggcacgag tacatgatca aggccttcgg
    15601 tggcaaggga tatcaggcga cgacacccgc cgaggtcggc gccgcgctgc gggaggccct
    15661 cgcatccggc aggcccgcgc tcatcgactg cgtcatcgac ccctcggacg gaaccgagag
    15721 cggcaacatc gcgcacctga accccaaggg catcaccgca aagcactgag ctctcccgca
    15781 ccgacacgat caacaaacga cgaaaggaaa cgtgtcatga ccgatttgcc gctctccggg
    15841 atcaaggtga tcgacttcac cggggtccag gccggcccgg cgtgcaccca gatgctcgcc
    15901 tggtttggcg ccgacgtcct caaggtcgag cgcacgagcg ggggtgacgt cacgcgtaat
    15961 cagttgcgcg acatccccga actggacgcg ctgtacttca ccatgctcaa cagcaacaag
    16021 cgttcactgg cgatcaacac gaagtcaccc gagggactcg aggtgatgga gaagctgatt
    16081 cgcggtgccg acgtgctcgt cgagaacttc gcacccggcg ccatggaccg catggggctg
    16141 tcgtgggaca agatccagga atggaacccg cggctgatct tcggctccgt caagggattc
    16201 aacgacgact cgtcgtggaa cgacctgaag gtctacgaga acgtcgccca gtgcgccggc
    16261 ggcaacgcct cgaccaccgg gttctgggac gggccaccga ccgtggccgg cgctgccctc
    16321 ggtgacagca acaccggcat gcacctgctg atcggcatcc tcactgcgat cattcagcgc
    16381 gacaagaccg gcaagggtca gaaggtgtcg gccgcaatgc aggacgccgt gctcaacctg
    16441 tgccgtgtca agctgcgcga ccagcagcgg ctggaaaagg tcggctacct ggaggagtac
    16501 ccgcagtggc ccaatggtga gttcggtgat gccgttcccc ggggcggcaa cgcgggcggc
    16561 ggtggccagc ccggctgggt cgtcaagtgc aaaggctggg agaccgaccc caacgcctac
    16621 atctacttca ccatccagga gcagaactgg aagcgcaccg ccgaggccat tggtcggccg
    16681 gagtgggtgg aggacccggg ctacaacacc gcacgtgccc gccaggacaa gatcttcgac
    16741 atcttcgccg agatcgagaa atggctggcg gacaagacga agtgggaggc ggtcgacatc
    16801 ctgcgtgagt gggaggtgcc gtgtgcgccg gtcatgtcca tgaaggacct cgccgaggat
    16861 aaggatctgc gcaagagcgg aagcatcgtc gaggtggagc agaagggccg gggtacctac
    16921 ttgaccgtcg gcagcccgat caagttctcg gagttcaagc ccgacatcaa gggtgcgccg
    16981 ctgctcggcg agcacaccga cgaggtgctt gccgagttgg gctacgacgc ggacaccatc
    17041 gccgcatggc acgagaagaa gatcgtcgtc taacccgccc gcccgggtga gaaccgcccc
    17101 aggtcccctc ctggggcggt tccgcccttt ctcttaggag gcgtccgcca tgagcatgac
    17161 ccttgtccga gagattgccg ccagccatcg aggccagcgt ggtgcactgc ttccgatcct
    17221 gcatgcggtt caggaggcct tgggatgtgt gccgccggag gcgattccgg tgttggctga
    17281 cgaactcaac ctctcccgtg ccgatgtgca tggggtggtc tcgttctacc acgacttccg
    17341 ttctgcgcct gccggtcgca ccaccgttcg catctgccgg ggcgaggcct gtcaggcggt
    17401 tggagccgag cgactcgtga gccagctgca ggacaggtgc ggtatgtcgc tgggggagac
    17461 cagccgcgat ggctcgctga ccgtcgagca ggtcttttgc ttgggcaact gtgccttggg
    17521 ccccgccgcg caagtcaacg gccggctccg cggccgactg gacgaggcgc gcctgtcggc
    17581 tatcgtcgat gaggcggtcg tgcaatgagc gcgccggtga cggtgtatgt gccgagcgat
    17641 tccgcggcgc ggtccgtcgg tgccgacgaa gtcgctgacg ccctggcgag cgccgccgca
    17701 cgtcagggac gctcgattcg attggtccgc aacggttctc gcgggctatt gtggctggag
    17761 ccactggtcg aggttgagac ggaggacggg cgcatcggct acggtccggt ggccgcgtcc
    17821 gatgtcgacg gcctggtgtc cggcggattg ttcgacgggg ccgagctccc acagcggttg
    17881 ggagtggttg acgaattgcc ctggctcgcc gcgcagaatc gggtcacgtt cgccaggatg
    17941 ggcgtgaccg atccgttgtc gcccgatgac tacctgcgcc acggcgggat ggtgggactt
    18001 gttcgcgcac tggagcgttc gcccgaggat gtcgtcgcgg aggtcaccga ctccggcctg
    18061 cgcggccgcg gcggggcagg ctttcccgca ggcatcaaat ggaagaccgt gcacgactgc
    18121 gttgacgcgc tgaagttcgt ctgctgcaac gccgacgaag gcgacagcgg caccttcgcc
    18181 gaccggatgc tgatggaggg cgatccgttc ctgctcatcg agggcatgac gatcgcggcc
    18241 tacgcagtcg gcgccaccga gggctacgtc tacatcaggt ccgagtatcc cgaggcggtg
    18301 gccacgatgc gtgccgccat cgagatcgcc tacgggcgag ggtggctcgg cgacaacgtg
    18361 ctgggctcgt cgctgaactt cgacctccag gtacgggtgg gcggcggagc ctacatctgc
    18421 ggcgaggaaa cctccatgct ggaaagcctc gagggcaagc gcggaatggt gcgcgcgaag
    18481 ccgccgattc cggccatcaa tggtctgttc ggcaagccga ccgtcgtgaa caacgtgctc
    18541 acactcgcca ccgtgccgac ggtcctcgcc cacggtgcgc aggcgtatca gcaactcggc
    18601 gtggagcgtt cgcgcggcac acaggtgttc cagctcggcg gaaacatcaa acacggcggc
    18661 atcgtcgaga cggcgttcgg gatcacgctc ggcgagctcg tcgacggcta cggcggcggc
    18721 acccggtccg gtcggcccgt gcgtgcggtc caggtcggcg ggccgctcgg ggcatatctg
    18781 ccgagagcca agttcgacct gccgatggac tacgaggcgt tcgtcgccgc gggtgcgatg
    18841 gtcggccatg gcggcatcgt ggtgttcgac gaaaccgtcg acatggcggc ccaggccagg
    18901 tttgcgatgg agttctgcgc ggccgagtcg tgtggcaaat gcacgccctg ccgcgtcggt
    18961 gccgtgcgcg gcgtcgaggt catcgaccga atcacagcgg gcgagcaccg cgacgaaaac
    19021 ctggccctgc tcgaggacct gtgcgacgtg atgaccgagg gctcgttgtg cgccatgggc
    19081 gggttgaccc cgatgccggt gcgcagcgcg atcgaacatt tcccagacga cttctatgcc
    19141 aggcacgcaa gcgcgaagac ggaaggtaaa ccatgacgtt gctcaaggaa cccgacttcg
    19201 gcaccccggc caaggacggt ccggcgtcgg tctcgctcga ggtcgacggg atgcgcgtct
    19261 ctgttcctga aggcacatcc gtgatgcggg cggcggcgat ggccggcgtc gacgtcccca
    19321 agttgtgcgc cacggaccgg ctcgaagcgt tcggctcctg ccggctgtgt ctggtcgaga
    19381 tcgacggccg ccgtggcact cccgcgtcgt gcacgacacc ggtcgccgac gggatggtcg
    19441 tacatactca gacgccgaag gtcgccaagc tccgtgagaa cgtcatggag ctctacatct
    19501 ccgaccatcc gctcgattgc ctcacctgcg ctgccaacgg tgactgcgaa ctgcaggaca
    19561 tggcgggggc ggtcggcctg cgtcaggtca ggtacggcta cgaaggtgag aaccacctcg
    19621 acgcggtcaa agaccaaagc aatccgtact tcgacttcga cccgtccaaa tgcattgcct
    19681 gttcccgttg tgtgcgggcc tgtgacgaga ttcagggcac gttcgcgctt accatcgaag
    19741 gccggggctt cgactcgaag gtgtcacccg gtgcgggcga gtcgttcatg gactcggagt
    19801 gtgtgtcgtg cggcgcctgc gtgcaggcgt gcccaacggc caccctgcag gagaagtcag
    19861 tgatccaact ggggatgccg tcccggtcgg tgatcaccac gtgcgcctat tgcggcgtcg
    19921 gctgctcgtt caaggcggag ttgcgcggtg acgaggtggt ccgcatggtg ccccacaagg
    19981 atggcggcgc taacgagggc cactcgtgtg tcaaggggcg gttcgcctt
//
LOCUS       DASRJA010000041         2468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_799364,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000041 DASRJA010000000
VERSION     DASRJA010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799364"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFC01_01895"
     CDS             <1..325
                     /locus_tag="VFC01_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HZN78428.1"
                     /translation="PSPRAMKRLRDKVRDRTGRNRVGWDIREVIADLNPVLRGWGNYF
                     RTGNAANKFTQVDDHVVWRLKRLMIKKRGRNLRAGQAQQWNEDWFNGHGLHRLRGTIR
                     YPKTA"
     gene            435..1160
                     /locus_tag="VFC01_01900"
     CDS             435..1160
                     /locus_tag="VFC01_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HZN78429.1"
                     /translation="MTASQHTIAGTVLTMPVKIRSANQHMAMFSVDADAAQQLIDYSG
                     LQVCRYIPGRAIVVLMLMHYLDGDLGQYLEYGTNVMVNPPGSNANGPRALQDAGAFIH
                     HLPVNQAFTLEAGKTIWGYPKVMADFTVRDARQFGFDVSIDGQLAVGMEFQPGLPIPS
                     AFTSKEQVYRTYSHRDGVTRETLGHTKLSGVRYRLGGARVWLGDHPYAKELAALGLPK
                     RALVSSSAANVEMSFDDAQEVRP"
     gene            1157..2356
                     /locus_tag="VFC01_01905"
     CDS             1157..2356
                     /locus_tag="VFC01_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN78430.1"
                     /translation="MTTTKPDVDLTDGAFYAGDSRSIYKWMRENEPVFRDRNGLAAAA
                     TYAAVIEAERNPELFSNAGGIRPDQPGVEMMIEMDDPQHLLRRKLVNAGFTRKRVKDL
                     EGSISLLCDTLIDAVCERGECDFVWDLAAPLPMAVIGDMLGVRPDEREMFLKWSDDLV
                     SSLSSTAAEADIQVTMDAFATYSTYMTGMIEARKTEPTDDLVSVLVHAEVEGSKLEDH
                     QIVTEVLLLLIGGDETTRHTLSGGTRQLLLHPDQHQRLVKDLDLLPNAIEEMLRWTAP
                     VKNMARTITADTEFHGTALREGEKMILLFESANFDEKVFEDPESFNIERYPNNHLAFG
                     FGTHFCLGNQLARLELSIMQTKLLQRLPDMRLASDAELPLRPANFVSGLESMPVKFTP
                     TNRVVGG"
BASE COUNT          490 a          780 c          784 g          414 t
ORIGIN      
        1 gccgtcgccg cgggcgatga aacggctgcg agacaaggtc cgggaccgca ccggccgcaa
       61 ccgtgtcggg tgggacatcc gcgaggtgat cgcggacctc aatcccgtcc tgcgcggctg
      121 gggcaactac ttccgcaccg ggaacgccgc caacaagttc acacaggtcg acgaccacgt
      181 ggtctggcgc cttaagcgcc tgatgatcaa gaaacgtggc cggaacctgc gcgctgggca
      241 agcccagcag tggaacgagg actggttcaa cgggcacggc ctgcaccggc tccgcggcac
      301 catccgctac ccgaagactg cgtaaccatg tcaagaagat catcggtaag ccgtgtgcgg
      361 gaaaaccgca cgcacggatt gaaaggggga tggggaaccg gatccgctcg cggacaccgc
      421 gcccctgact accaatgact gcatcgcagc acacaatcgc cggcacggtg ctgaccatgc
      481 cggtgaagat ccgctcggct aaccagcaca tggcgatgtt ctcggtggac gccgatgccg
      541 cacagcaact gatcgactac agcggcttgc aggtctgccg gtacataccg ggtcgcgcca
      601 tcgtggtcct gatgctgatg cactacctcg acggcgacct gggtcagtac ctcgagtacg
      661 gcacgaacgt gatggtcaat ccgcccgggt cgaacgcgaa cgggccacgg gcccttcagg
      721 atgcgggggc tttcatccat caccttccgg tgaaccaggc gttcacattg gaagcgggta
      781 agacgatttg gggctatccg aaggtgatgg ccgacttcac cgtgcgcgat gccaggcagt
      841 tcggattcga cgtcagcatc gacggtcaac tggccgtggg catggagttc caacctggcc
      901 tgccgattcc gtcggcgttc acgtccaagg agcaggtgta ccgcacgtac tcgcatcgcg
      961 acggcgtcac ccgcgaaacg ctcgggcaca caaagctttc cggtgtgcgc taccggctcg
     1021 ggggtgcgcg ggtgtggctc ggcgaccacc cgtacgcgaa ggaactcgcc gccctaggcc
     1081 tgcccaaacg cgcccttgtg tccagttctg ctgccaacgt agagatgtcg ttcgacgacg
     1141 cgcaggaggt tcgaccgtga cgacaaccaa accggatgtc gatctcactg acggcgcgtt
     1201 ctacgccggt gactcgcggt cgatctacaa atggatgcgc gagaacgaac cggtgttccg
     1261 ggaccgcaat ggcttggctg ccgccgcgac gtatgcggcc gtgatcgagg ccgagcgcaa
     1321 tccggagctg ttctccaacg ccggtggcat ccgacccgat cagcccggcg tcgagatgat
     1381 gatcgagatg gacgaccccc agcatctgtt gcggcgcaag ctcgtcaacg ccggcttcac
     1441 ccgcaaacgc gtgaaagacc ttgaggggtc gatcagtttg ctgtgcgaca cgctgattga
     1501 cgcggtatgt gagcgagggg agtgcgactt cgtctgggat ctcgccgcgc cgcttccgat
     1561 ggcggtcatc ggcgacatgc tcggcgtgcg ccccgacgaa cgcgagatgt tcctcaagtg
     1621 gtccgacgac ctggtcagct ccttgagtag cactgcggcc gaagcggaca tccaggtcac
     1681 gatggacgcc ttcgcgacct acagcacgta catgacgggc atgatcgaag cccgcaagac
     1741 ggagccgacc gacgatctgg tgagtgtcct cgtgcatgcg gaggtggagg ggtcgaagct
     1801 cgaggaccac cagatcgtca ccgaggttct gctccttctg atcggcggcg acgagaccac
     1861 ccgtcacacg ttgtcaggcg gcacaaggca attgctgctt caccccgatc agcatcagcg
     1921 gctcgtcaag gacctcgacc tgctgcccaa cgcgatcgaa gagatgctgc gctggaccgc
     1981 gcccgtcaag aacatggcgc gaaccatcac cgcggacacc gaattccacg gcaccgccct
     2041 gagagagggc gagaagatga tcctgctgtt cgagtcggcc aacttcgacg agaaggtgtt
     2101 cgaggatccg gaaagcttca atatcgaacg ctacccgaac aaccacctcg cgttcggctt
     2161 cggaacgcac ttctgtttgg gtaaccagct tgcccggctg gagctttcga tcatgcagac
     2221 gaaactgctg cagcgcctgc ctgatatgcg actggcgtcg gatgcagagc tgccgctgcg
     2281 cccggcaaac ttcgtcagcg gcctggagtc gatgccggtc aaattcaccc cgaccaaccg
     2341 agtggtcggc gggtagcgag gaccgtcaca ggtcacggcc gtaggtcgcc gcgggtgcat
     2401 acactcgcgc tcatggccgg gctgctgcag atcgaggact gcctcgacgc cgacggcggc
     2461 gtcgtact
//
LOCUS       DASRJA010000042         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4191114,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000042 DASRJA010000000
VERSION     DASRJA010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4191114"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFC01_01910"
     CDS             complement(<1..294)
                     /locus_tag="VFC01_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78431.1"
                     /translation="MTSWLKAKLGRMRVSTLVLIVAFVALFWVYHNFEPRLTPAEAPT
                     TAVVPPGFVPDPNYTWVPRTNVRRPKEPETTTMTTTPTTTSPTETTTTSPGETT"
     gene            complement(291..1271)
                     /locus_tag="VFC01_01915"
     CDS             complement(291..1271)
                     /locus_tag="VFC01_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZN78432.1"
                     /translation="MTNSTVLAVSFDERWHGFWAGNIGVWVLDRGVPIALYLIGGLLA
                     ARFINWGARRIVRRIDAEYQESDQLVRTESAKHRQAVASVISWVTVALLFVIVIVQVT
                     SILAIPVGSLVAPAAVLGAALGFGAQRVVQDLLSGFFIITEKQYGFGDLVSLTVTGIT
                     APAEGTVEDVTLRVTKLRSTEGEMFTIPNGQIVKTLNLSKDWARAVIDIPVPASADLN
                     VVNDVLHGVAEKAMEETSLRALLLDKPQLMGVESIELDTVNLRMVARTLPGKQFEVGR
                     RIRVLVVRALRRAGVASPTDGNTPMVEAIVHPATAGGAEEETQGPTEPKK"
     gene            complement(1261..2376)
                     /gene="prfB"
                     /locus_tag="VFC01_01920"
     CDS             complement(1261..2376)
                     /gene="prfB"
                     /locus_tag="VFC01_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZN78433.1"
                     /translation="MDPDRQADLAALDSTLTTVERVLDVEGLRGRIEKLEHEASDPHL
                     WDDQAHAQKVTSELSHTQGELRRVEDLRQRLDDLPVMYELAIEEGGAEGDDANAEADA
                     ELKKLREDIEAMEVRTLLSGEYDEREALVNIRSGAGGVDAADWAEMLMRMYIRWAEKH
                     NYPVEVFDTSYAEEAGIKSATFAVHAPFAYGTLSVEQGTHRLVRISPFDNQGRRQTSF
                     AEVEVLPVTETTDHIDIPESDVRVDVYRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQ
                     NEKSQLQNKVSAMRVLQAKLLERKRLEERAELDALKGDGGSSWGNQMRSYVLHPYQMV
                     KDLRTDYEVGNPAAVLDGDIDGFLEAGIRWRNRRDDE"
     gene            2478..>3553
                     /locus_tag="VFC01_01925"
     CDS             2478..>3553
                     /locus_tag="VFC01_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN78434.1"
                     /translation="MRPYHVAIVGSGPSGYFAAASLLKFADSNDDADVRVDMLEMLPT
                     PWGLVRSGVAPDHPKIKSISAQFEKTALDPRFRFFGNIVVGDHVQAAELAERYDAVVY
                     AVGAQSDRSLGIPGEDLPGSIAAVDFVGWYNAHPHFEEMAPDISSGRAIVVGNGNVAL
                     DVARILVTDPDVLATTDIADHALKALHDRGVEEVLIVGRRGPLQAPFTTLELRELGDL
                     EGLGDVDVIVDPADFADISDEDLEAAAKTVRNNIKVLRGYAEREPRGAKRRIVFRFRT
                     SPIEIKGEGRVESIVLGHNELVDDNGRIIAKDTGEREEVPAQLVVRAVGYRGVPTPGL
                     PFDERGGTIPHSGGRIDGSSNEYVV"
BASE COUNT          589 a         1184 c         1134 g          646 t
ORIGIN      
        1 cgtggtctcc ccaggtgacg tcgtggtcgt ctccgtcggg ctggtggtag tcggcgtagt
       61 cgtcatggtg gtcgtctcgg gctccttggg cctgcgcacg ttggtgcgcg gcacccaggt
      121 gtagttcggg tcgggtacga agccgggcgg gacgaccgca gtcgtcggtg cttccgcggg
      181 cgtcagccgc ggctcgaagt tgtggtacac ccagaacagc gcgacgaacg cgacgatgag
      241 caccaacgtc gaaacccgca tccggccgag tttggccttc aaccaggacg tcatttcttc
      301 ggctccgtcg ggccctgcgt ctcttcctca gcgccacctg ccgtcgccgg atgaacaatg
      361 gcctccacca tcggcgtatt gccgtccgtg ggcgaagcca cacctgcccg acggagggcc
      421 cggacgacca gcacgcgtat ccgccgcccc acctcgaact gcttgcccgg cagcgtccgc
      481 gcgaccatcc gcaggttgac cgtgtcgagc tcaatgcttt ccacgcccat gagctgcggc
      541 ttgtccagca gcagcgcgcg aaggctcgtc tcctccatcg ccttctcggc taccccgtga
      601 agcacgtcgt tgaccacatt gaggtcggcc gatgccggga ccgggatgtc gatgaccgcc
      661 cgcgcccagt ccttggacag gttgagcgtc ttgacgatct gaccgttggg gatggtgaac
      721 atctcgccct cggtcgaccg cagcttggtg acgcgaagcg tgacatcctc cacggtgcct
      781 tcggctgggg cggtgatgcc ggtgaccgtg agggacacca ggtcaccgaa cccgtactgc
      841 ttctcggtga tgatgaagaa cccggagagc aggtcctgca ccacgcgctg cgcgccgaaa
      901 ccgagcgcgg cgcccagcac cgcggcgggc gcgacgaggg atccgaccgg gatcgcgagg
      961 atcgaggtga cctgcacaat cacgatgacg aaaagcagcg ctaccgtcac ccaggagatc
     1021 accgaggcga cggcctggcg gtgcttggcg ctctccgtgc gcaccagctg atcgctttcc
     1081 tgatattcgg cgtcgattcg gcgcacgatt cgtcgagcgc cccagttgat gaaccgggcc
     1141 gccagtaggc cgccgatcag atacagcgca atcggcacgc cgcggtcgag aacccagacg
     1201 ccgatgttgc cggcccaaaa gccgtgccac cgctcgtcga aactcactgc aagtacggtg
     1261 ctattcgtca tctcgcctgt tgcgccaccg gattcccgct tcgaggaacc cgtcgatgtc
     1321 tccgtccaga acggccgccg gattgccgac ctcgtagtcg gtgcgcaggt ccttcaccat
     1381 ctggtacgga tgcaacacgt aggaccgcat ttggttgccc cacgagctgc cgccgtcacc
     1441 cttcaacgcg tccagctcag cacgttcttc taaacgcttg cgttccaaca actttgcctg
     1501 aagcacccgc atcgctgaca ctttgttctg cagctgcgac ttttcgttct ggcaagtcac
     1561 gacgataccc gttgggatgt gagtgagtcg aacggcagag tcggtggtgt tcacagactg
     1621 accgccgggg ccgctggatc ggtagacatc aacgcgcaca tcgctttccg gaatgtcgat
     1681 gtggtcagtg gtttcggtga ccggaagcac ttcgacttcg gcgaacgacg tctggcggcg
     1741 gccttggttg tcgaatgggc tgattcgaac caagcgatga gtgccctgct caactgacaa
     1801 cgtcccatat gcgaacggtg cgtggactgc gaatgtcgcg ctcttgatcc cggcctcttc
     1861 cgcataggag gtgtcgaaca cctcgacggg atagttgtgc ttttccgccc agcggatata
     1921 catccgcatc agcatctcgg cccagtccgc ggcgtccact ccgccggcgc cggagcggat
     1981 gttcaccagc gcctcgcgct cgtcgtattc gccggacagc agcgtgcgca cttccatcgc
     2041 ctcgatgtct tcgcgcagct tcttcagctc ggcgtcggcc tcggcgttcg catcgtcgcc
     2101 ctctgcccct ccttcttcga tggccagctc gtacatcacg ggcaggtcgt cgaggcgctg
     2161 ccgcaggtct tccacccggc gcagctcccc ctgcgtgtgc gacagctcac tggtgacctt
     2221 ctgggcatgc gcctggtcat cccacagatg cgggtcggac gcctcgtgct ccagcttttc
     2281 gatccgaccg cgaagcccct cgacatcgag cacccgctcc accgtggtca gggtggagtc
     2341 gagggcggcg aggtcggctt gacggtcggg atccacggga gttcagcgta ccggcggttt
     2401 aacatcggac tggcaaccag cctggccacg acgcgacagc cccttgcgga tggccgggca
     2461 gcgacagaaa gctgtgtatg cgcccatacc atgtggcgat tgtcggctca ggcccctcgg
     2521 gctactttgc cgcggcctcg cttctgaagt tcgccgactc aaatgacgat gccgacgttc
     2581 gcgtcgacat gctggagatg ctgccgaccc cgtgggggtt ggtgcgctcc ggcgtcgccc
     2641 cggaccatcc gaagatcaag tcgatcagcg cccagttcga gaaaaccgcg ctggatccgc
     2701 ggtttcggtt cttcggcaac atcgtggtgg gcgaccatgt gcaggccgca gagctggccg
     2761 agcgctacga cgcggtggtc tacgccgtgg gcgcgcagtc cgaccggtcg ctgggcattc
     2821 ccggcgagga cctgcccggc agcatcgcgg ccgtggattt cgtcggctgg tacaacgctc
     2881 acccgcactt cgaggaaatg gcgcccgata tttcaagtgg gcgggccatc gtggtcggca
     2941 acggcaacgt cgcgctggac gtggcgcgca tcctggtcac cgatcccgac gtgctggcca
     3001 cgacggatat cgccgatcac gcgttgaagg cgctgcacga ccgcggcgtc gaggaggtgc
     3061 tgatcgtcgg ccggcgcgga ccgctgcagg ccccgttcac cacgctcgag cttcgcgaac
     3121 tcggcgatct ggaaggtctc ggcgacgtcg acgtcatcgt cgacccggcc gacttcgccg
     3181 acatctccga cgaggacctc gaggcagcgg ccaagaccgt ccgaaacaac atcaaggtgc
     3241 tgcgcgggta cgccgaacgc gagccccgcg gcgccaagcg tcgcatcgtg ttccggttcc
     3301 gcacctcacc gatcgagatc aagggcgagg gccgcgtcga atcaattgtg ttgggccaca
     3361 acgagttggt cgatgacaac ggccgcatca tcgccaagga caccggcgag cgcgaggagg
     3421 tgcccgctca gctggtggtg cgggcggtcg gctaccgcgg cgtgccgacg ccggggctgc
     3481 ccttcgacga gcgcggtggc acgatcccgc atagcggcgg ccggatcgac ggcagcagca
     3541 acgagtacgt cgt
//
LOCUS       DASRJA010000043         8556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_529591,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000043 DASRJA010000000
VERSION     DASRJA010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529591"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /gene="secF"
                     /locus_tag="VFC01_01930"
     CDS             complement(<1..1164)
                     /gene="secF"
                     /locus_tag="VFC01_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZN78435.1"
                     /translation="MAGKHSADVTDSTGVEAPDLEAAEAAAPKHGFFTRLYTGTGAFE
                     VVGRRKMWFTISGLIVAVAIASIVLRGFTFGIDFEGGTKVSMPAASATGTVSTQQVED
                     VFSKTLGKAPESVVAVGSGGSATIQIRSETLTNEETEKLRNGLFDAFQPKGSDGQPSK
                     QAISDSAVSETWGGQITKKALIALVVFLVLVTIYITVRYERYMAMSALLTMFFDLTTT
                     AGVYSLVGFEVTPATVIGLLTILGFSLYDTVIVFDKVEENTHGFEHTTRRTFAEQANL
                     AINQTFMRSINTSLISVLPIIALMVVAVWLLGVGTLKDLALVQLVGVIVGSYSSIFLA
                     TPMLVTLRERTELVRTHTRRVLNRRKPAKAEVVQDAAPEEVAAVAAKPAPEKPA"
     gene            complement(1169..2953)
                     /gene="secD"
                     /locus_tag="VFC01_01935"
     CDS             complement(1169..2953)
                     /gene="secD"
                     /locus_tag="VFC01_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZN78436.1"
                     /translation="MASSSAPVHPARYLTLFLVLLVGVYLLVFLTGNKEAKPKLGIDL
                     QGGTRVTLTARTPDGSAPTREALDQAQQIISARVNGLGVSGSEVIIDGSNLVITVPGN
                     DSSEARNLGQTARLYIRPVIHVIPAEGQGQQPPGATPPGAPPGAPPGAPVPGLPPGAP
                     AEPGAPAEPGAPASPPSPAEQAPPPATPAPQPRPYPQEPPATPTPAPPTPGAPPAPGT
                     PPPAEKQTLAQRIADEKQLRQSTDQRIQILALQFQAIRCSQEDVLAGNDDPNLPLITC
                     SQDGKQVYLLDKAIIKGEQIKNADSGLDQQRGEYVVTVEFNDEASRIWADFTAANVGT
                     QTAFVLDSKVVSAPEIQEAIPGGRTQITGQFTADSARELANVLKYGSLPLSFESSEAE
                     TVSATLGLTSLRAGLIAGAIGLALVLLYSLIYYRVLGLLTALSLVASGAMVFAILVLL
                     GRYINYTLDLAGIAGLIIGIGTTADSFVVFFERIKDEIREGRSFRSAVPRGWARARKT
                     ILSGNAVSFLAAAVLYFLAVGQVKGFAFTLGLTTILDVVVVFLVTWPLIYLASKSPTL
                     AKPAFNGLGAVQQIARERRAAAHATGRG"
     gene            complement(3020..3379)
                     /gene="yajC"
                     /locus_tag="VFC01_01940"
     CDS             complement(3020..3379)
                     /gene="yajC"
                     /locus_tag="VFC01_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZN78437.1"
                     /translation="MDLVVFLPLLIILGAFMFFASRRQKKAMQATIDLHNSLKVGDRV
                     HTTSGLQATITGITDDNVDLEIAPGVVTTWMKLAVRDRVVDDIEDDTDDTDDTVDYEP
                     ESSATEVTETPNRKTDG"
     gene            3520..3930
                     /locus_tag="VFC01_01945"
     CDS             3520..3930
                     /locus_tag="VFC01_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HZN78438.1"
                     /translation="MLGHIGINVPDLDGARRYYSAVMPLVGFEVFLDAEDEFAYRPAA
                     SKPGTFLFFYPSAEQSAYSRLQTGLQHLAFMVRTRSAVAAVHSAAAELAGRFGGRVLQ
                     EPQIFPQYPQPYFATFWLDPWGFMLEAVCHYDRD"
     gene            complement(3942..5471)
                     /locus_tag="VFC01_01950"
     CDS             complement(3942..5471)
                     /locus_tag="VFC01_01950"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HZN78439.1"
                     /translation="MTGSTEADPNIKSDAEVYPLGKSRPPLAVDDLPEPEEVFKVSRL
                     GPLQIVQLVIGPSLIALGISIGSGEWLLGPQAVGEFGFIGIGWVITVSAILQTFYNVE
                     VSRYVVATGEVPIVGWGRVPPGWKFWIPFSLIVFYFAFIFGGWAASAGQGLFALIVGR
                     VHRPDELEYTRLLAIGLLFVVFLITVLARKISRALELSNWVMVGTLLAVLIVVDIAIV
                     PPSVWWDGIRGLLTPATPPAGITATTLGALAGFTALASGLNWYAMAHYRDHGYGMGHR
                     VGFLAGVRGERRELLAVGVTFRDTPENGSKWKRWYRLLLIDQWAVFFCGAMLGMLLPT
                     ILMAHIVATTGEAPTTKNVPTFAAEKLGDQYGSGMFYVMLVVGVLILFSTQLGIFEAL
                     VRNFTDAAHATSPRLRGLLEGDPRKFYYPYMLVVLVVIALAIHLTTPVRLVQISANMS
                     NLGALMFPFMLMYLNSKLPRPARPRWWHYAILVLNFLFFGFFFMNFVYELVSGSPLVK
                     F"
     gene            5678..7018
                     /gene="gabT"
                     /locus_tag="VFC01_01955"
     CDS             5678..7018
                     /gene="gabT"
                     /locus_tag="VFC01_01955"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HZN78440.1"
                     /translation="MSTLEQSRHLATAIPGPRSQELIDRKSAAVSRGIGNTMPVYAAR
                     AFGGIVEDVDGNRLIDLGSGIAVTTIGNSSPRVVDAVQDQVANFTHTCFMITPYEGYV
                     AVAEQLNRLTPGGGEKRSALFNSGSEAVENAIKIARTYTRKQAVVSFDHAYHGRTNLT
                     MALTAKSMPYKHGFGPFAPEIYRAPLSYPFRDAEYGKELATDGELAAKRAIGVIEKQV
                     GADNLAAVIIEPIQGEGGFIVPADGFLPALLDWCHENNVVFIADEVQTGFARTGAMFA
                     CEHEGIEPDLICTAKGIADGLPLSAVTGRADIMDAPHVSGLGGTYGGNPVACAAALAT
                     IETIEADGLVERARQIEVLMKDRLGRLQVDDDRIGDVRGRGAMIAMELVKPGTLEPDA
                     ELTMKLCVGCHTAGVIVLSCGTFGNVLRFLPPLAISDELLIEGLDVLALVLKDL"
     gene            7035..8468
                     /locus_tag="VFC01_01960"
     CDS             7035..8468
                     /locus_tag="VFC01_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HZN78441.1"
                     /translation="MTKVQNFIGGELVDSVSGGTMPLVDPTTGEEYATAPISNEQDID
                     NAYAAAAKAFADWKRTTPSHRQKALLDFADDVEKNAQALVEAEGRNTGKPNHVTMAEE
                     IPPMVDQIRFFAGAARMLEGKSAGEYMENHTSWIRREPVGVIGQVSPWNYPMMMAIWK
                     FCPAIAAGNTVVIKPSDTTPVTTVMLAEIASRHLPPGVLNVVCGDRNTGAAVVAHPTP
                     QMVSITGSVAAGRAVAVSAGGNLKRTHLELGGKAPVIVFDDSDIASAAEGIATAGYFN
                     AGQDCTAATRVLARASVASDLTDALAEQAKGATTTFSKATDDEDAWVPPVNNINQMER
                     VLSFFTDVPSHAKVATGGQRQGDKGFYVEPTVIGGLLQDDRLVQQEIFGPVITVQAFS
                     DEDEAVAWANGTEYGLASSVWTKDVSRALRVSAALDFGCVWINTHIPLVAEMPHGGYK
                     SSGHGKDLSMYGFDDYTRIKHVMAYTG"
BASE COUNT         1531 a         2728 c         2838 g         1459 t
ORIGIN      
        1 cgcaggcttc tccggtgccg gtttggcggc cacggccgcg acctcctccg gcgcggcgtc
       61 ctgcaccacc tcggctttgg caggcttgcg ccggttgagc actcgccggg tgtgcgtgcg
      121 gacgagctcg gtgcgctcgc ggagcgtcac cagcatcggg gtggccagga agatcgacga
      181 gtagctgccg acgatcacgc cgaccagctg caccagtgcc agatccttga gcgtgccgac
      241 cccgagcagc cacaccgcga cgaccatcag cgcgatgatc ggcagcaccg agatcaggct
      301 ggtgttgatc gaccgcatga acgtctggtt gatcgccagg ttggcctgct cggcgaaggt
      361 gcggcgggtg gtgtgttcga agccgtgggt gttttcctcg accttgtcga acacgatgac
      421 ggtgtcgtag agcgagaagc cgaggatggt cagcaggccg atcacggtgg ccggggtcac
      481 ctcgaagccg acgagcgagt acacgcccgc ggtggtggtc aggtcgaaga acatcgtgag
      541 aagcgccgac atcgccatgt accgctcgta gcgcacggtg atgtagatgg tgacgagcac
      601 caagaacacc accagcgcga tgagcgcctt cttggtgatc tggccgcccc atgtctccga
      661 caccgccgag tcgctgatgg cctgcttgct cggctggccg tcggagccct tcggctggaa
      721 cgcgtcgaac aacccgttgc gcagcttttc ggtttcctcg ttggtgagcg tctccgagcg
      781 gatctggatg gtggccgagc cgccgctgcc gacggcgacg accgattcgg gcgccttgcc
      841 caacgtcttg ctgaagacgt cctcgacctg ctgcgtgctg acggtgcccg tcgcactggc
      901 ggcaggcatg gacacctttg tgccgccctc gaagtcgatg ccgaaggtga agccgcgtag
      961 cacgatgctc gcgatcgcca ccgcgacgat caggccgctg atcgtgaacc acattttgcg
     1021 tcggccgacg acctcgaacg cgccggtgcc cgtatagagc cgggtgaaga agccgtgctt
     1081 gggcgctgcc gcctcggcgg cttcgaggtc gggcgcctcg acgccggtgg aatctgtcac
     1141 gtcggctgaa tgcttgcccg ccatctggct atccccgtcc cgtcgcgtgt gcggccgctc
     1201 gtcgttcgcg cgcgatctgc tgcaccgccc cgagcccgtt gaacgccggc ttggccagcg
     1261 tcggcgactt cgacgcgagg tagatgagcg gccaagtcac caggaacacc acgacgacgt
     1321 cgaggatcgt ggtcaggccc agcgtgaagg cgaagccttt cacctggccg acggccagga
     1381 agtagagcac cgcggcagcc aggaagctga ccgcgttgcc ggacaggatc gtcttgcgcg
     1441 ccctggccca accgcgcgga accgccgagc ggaacgaacg cccttcgcgt atctcatctt
     1501 tgatgcgttc gaaaaacacc acgaacgagt cggctgtggt gccgataccg atgatcagac
     1561 cggcgatacc cgccaggtcg agcgtgtagt tgatgtatct gccgagcaac acgaggatgg
     1621 cgaaaaccat tgcgccggag gcgacgagcg acaacgcagt cagcagcccg agcactcggt
     1681 agtagatcag cgaatacaac aacaccaatg ctaaaccgat cgcgccggcg atgagcccgg
     1741 cgcgcagcga ggtcaaaccc agtgtcgccg agacggtttc ggcttcggaa gattcaaacg
     1801 agagcggcag cgacccgtac ttcagcacgt tggcgagttc gcgggcggag tcggcggtga
     1861 actggccggt gatctgcgta cgcccgcccg ggatggcttc ttggatctcc ggtgcgctga
     1921 ccaccttgga gtccagcacg aacgcggtct gggtgccgac gttggctgcg gtgaagtcgg
     1981 cccagatccg ggacgcctca tcgttgaact ccacggtgac gacgtactcg ccgcgctgct
     2041 ggtcgaggcc cgagtcagcg ttcttgatct gctcgccctt gatgatcgcc ttatccagca
     2101 gatagacctg cttgccgtcc tgcgagcagg tgataagtgg aaggttcggg tcgtcgttgc
     2161 cggccaacac gtcctcttgg ctacaacgga tggcctggaa ctgcagcgcc aggatctgga
     2221 tgcgctgatc ggtgctctgt cgcagttgct tctcgtcggc aatgcgctgt gccagtgtct
     2281 gcttctccgc gggcggcggc gtgccgggag cgggcggagc gcctggggtt ggcggtgccg
     2341 gtgtcggcgt ggcgggcggc tcctgcggat acggccgcgg ctgcggtgca ggcgtcgccg
     2401 gtggcggcgc ctgttcggcg ggcgacggcg gcgacgccgg ggcgccgggt tcggccggag
     2461 cgccgggttc ggccggagcg ccgggaggta ggccgggcac gggtgccccc ggaggcgcac
     2521 cgggcggtgc cccgggcggc gttgcgcccg gtggctgctg gccctgtccc tcggcgggaa
     2581 tcacgtggat cacgggcctg atgtacagcc gggctgtctg gccgaggttg cgggcctcgc
     2641 tgctgtcatt gcccggcacc gtgatgacca ggttggagcc gtcgatgatg acctcggacc
     2701 cggacacgcc aagaccattc acgcgggcgc tgatgatctg ctgggcctgg tcgagcgctt
     2761 cccgggtggg cgcggagccg tccggtgtgc gcgcggtcag cgtcacgcgg gtgccgccct
     2821 gcaaatcgat gccgagcttg ggcttggcct ccttgttgcc ggtcagaaac accagtaggt
     2881 agacgccgac gagcaacacc aagaagagcg tcaggtagcg ggcagggtgc accggcgccg
     2941 aagacgatgc cacgttctca gggtctcctt cgagatcagt tcgtcagcac cgcaaagggt
     3001 acgtgctggt gctgagtcgt cagccgtcag tcttgcggtt cggggtctcg gtgacctcgg
     3061 tggcagagga ctcgggttcg tagtccacgg tgtcgtccgt gtcatctgtg tcgtcctcga
     3121 tgtcgtcgac gacgcggtca cgcaccgcca gcttcatcca ggtggtgacg acgccgggcg
     3181 caatctccag gtcgacgttg tcgtcggtga tgccggtgat ggtggcctgc agccccgacg
     3241 tggtgtgtac gcgatcaccg accttcagcg aattgtgcag gtcgatggtg gcctgcatcg
     3301 ctttcttctg gcgccgcgac gcgaagaaca tgaacgcgcc cagaatgatg agcaagggca
     3361 ggaatacgac cagatccatg gcaacaacta tctttcattt tggtcttgtg gtcaggctcc
     3421 cagaccgcag gtcaggggcc tcgtcgttga ccagtgtgcc attgtcgccg ccgacggccg
     3481 agcccccggt cgcctaccag ctgctgttag cgtcgccaga tgctgggaca catcggcata
     3541 aacgtgcccg acttggatgg tgcgaggcgt tattacagcg cggtgatgcc gctcgtcggc
     3601 ttcgaagtat tcctcgatgc cgaggacgag ttcgcgtacc ggccggcagc cagtaagccc
     3661 ggcaccttcc tgttcttcta tccatccgcg gagcagtccg cgtactcgcg actccagacc
     3721 ggactgcagc acctggcatt catggtgcgg acccgctcgg cggtggctgc cgtccactcc
     3781 gcggccgccg aactcgctgg gcggtttggc gggcgtgtgc tgcaggagcc gcagatcttt
     3841 cctcaatatc cgcagccgta cttcgcgacg ttctggctcg acccgtgggg cttcatgctc
     3901 gaagcggtgt gccattacga ccgcgactga cggtcaatga cctagaactt caccaagggg
     3961 cttccgctga ccagctcgta gacgaagttc atgaagaaga acccgaagaa caggaagttc
     4021 agcaccagga tcgcgtagtg ccaccatcgt ggccgggccg gtcttggcag cttcgagttc
     4081 aggtacatca acatgaacgg gaacatcagc gcgccgaggt tcgacatgtt cgccgagatc
     4141 tgcaccagcc gcacaggcgt ggtcaggtgg atcgccaggg cgatgactac gagcacgacc
     4201 aacatgtacg ggtagtagaa cttgcgcgga tcgccctcca gcagtccccg caaccgcggg
     4261 ctcgtcgcgt gcgcggcgtc ggtgaagttg cgcaccagcg cctcgaagat gcccagctgt
     4321 gttgagaaca ggatgagcac gccgacaacc agcattacgt agaacatgcc cgacccgtac
     4381 tggtcaccca gtttctctgc cgcgaacgtc ggcacgttct tcgttgtcgg tgcttctccg
     4441 gtggtggcga cgatgtgggc catcaagatg gtgggcagca gcatgccgag cattgcgccg
     4501 cagaagaaca ccgcccactg gtcgattagc aggagccgat accagcgctt ccatttcgag
     4561 ccgttctccg gcgtgtcccg gaaggtgaca ccgacagcga gcagctcccg ccgctcacca
     4621 cgcaccccgg cgaggaagcc cacccggtgg cccatcccgt aaccgtggtc ccggtagtgg
     4681 gccatcgcgt accagttgag gccggacgcg agcgccgtga atccggcgag cgccccgagg
     4741 gtcgtcgcgg tgatgccggc cggtggtgtc gccggtgtga gcaggccccg gattccgtcc
     4801 caccacacag atggcggcac gatggcgatg tcgaccacga tcagtaccgc cagcagcgtg
     4861 ccgaccatga cccagttgct cagctcgagt gcgcgggaaa tcttgcgggc caacacggtg
     4921 atcaggaaga ccacgaagag caggccgatc gccagcagcc tggtgtactc cagctcgtcg
     4981 ggtcggtgca cccgcccgac gatcagcgcg aacaggccct ggcccgcgct cgccgcccat
     5041 ccgccgaaga tgaatgcgaa gtagaagacg atgagggaga acgggatcca gaacttccag
     5101 ccgggcggga cgcggcccca tccgactatc ggaacctccc cggtcgccac cacgtaccgg
     5161 gagacctcca cgttgtagaa ggtctgcagg atcgcggaca ccgtgatcac ccacccgatg
     5221 ccgataaacc cgaactcgcc gaccgcctgc gggccgagca gccactctcc actgccgatc
     5281 gagatgccca gggcgatgag gctcggcccg atcaccagct ggacgatctg cagcggcccg
     5341 agccgggaga ccttgaacac ttcctcgggt tcgggaaggt cgtccacggc gagcggcgga
     5401 cggcttttgc ctaagggata aacctcggcg tcggacttga tgttgggatc agcttcggtg
     5461 ctgccggtca tggcgacctc ccggacagag tcgacagcat tcttttcccg ttagcgccgc
     5521 ggtcaaaacc aagtgcgacg aaatccgttg cgacaacccg ttccggccaa cgatcccgtt
     5581 ctgaacgcga gggttttcaa tcggatcggt gcgtctcaga ctaggctcga accgcgatgc
     5641 ggcattcgtc caacccaccg gtcatcagga ggcttcattg agcactctcg agcaaagccg
     5701 ccatctcgcc accgcaattc ccggtccacg gtcgcaggag ctgatcgacc gcaaaagcgc
     5761 cgcggtgtca cgcgggatcg gcaacacgat gcccgtctac gccgccaggg cgttcggcgg
     5821 catcgtcgaa gacgtcgacg gcaatcggct catcgatctg ggctcgggta tcgctgtcac
     5881 gacgatcgga aactcgtcgc cccgcgtggt cgacgccgtg caggaccagg tcgccaactt
     5941 cacccacacc tgcttcatga tcacgcccta cgagggctac gtcgccgtgg ccgaacagct
     6001 caaccggctc acaccaggcg gcggcgagaa gcggtcggca ttgttcaact ccggttcgga
     6061 ggccgtggag aacgcgatca agatcgcccg gacgtacacg cgcaagcagg ccgtcgtctc
     6121 gttcgaccac gcctaccacg gccgcaccaa cctgacgatg gcgctgaccg cgaagtccat
     6181 gccgtacaaa cacggcttcg gcccattcgc ccccgagatc taccgcgcgc cgttgtccta
     6241 tccgttccgc gacgccgaat acggcaagga gctcgccacc gacggcgagc ttgccgctaa
     6301 gcgggctatc ggcgtcatcg agaaacaggt gggtgcggac aatctggccg cagtgatcat
     6361 cgaacccatc cagggtgagg gcggcttcat cgttccggcc gatggcttcc tgcccgcact
     6421 gctggattgg tgccacgaga acaatgtggt gttcatcgcc gacgaggtgc agacggggtt
     6481 cgcccgcacc ggtgcgatgt tcgcctgcga acacgaaggc atcgaaccgg acctgatctg
     6541 caccgccaag ggcatcgccg acggtctgcc actgtcggcg gtcaccggcc gcgccgacat
     6601 catggacgcc ccacacgtga gcgggctggg cggcacctac ggcgggaacc ccgtggcgtg
     6661 cgcggcggcg ctcgcgacca tcgaaaccat cgaggccgat ggtttggtgg agcgcgcgcg
     6721 ccagatcgaa gtgttgatga aggaccggct cgggcgtctg caggtggacg acgaccgcat
     6781 cggcgacgtg cgtggccgcg gcgcgatgat cgcgatggag ttggtcaagc cgggcacgct
     6841 cgagcccgac gcggagctca ccatgaagct gtgcgtcggg tgccacaccg ctggcgtaat
     6901 cgtgttgtcc tgcggcacat ttggcaatgt gctgcgtttc ctgccgccgc tggccatcag
     6961 cgacgagctg ctcatcgaag gcctcgacgt actcgctctc gtcctcaaag acctctaacg
     7021 aataaggagc tgacatgacc aaggttcaga atttcatcgg cggcgagctc gtcgactccg
     7081 tcagcggtgg caccatgccg ctcgtcgacc cgactaccgg cgaggaatac gccaccgcgc
     7141 cgatctcgaa cgaacaggac atcgacaacg catacgcggc cgctgccaag gcatttgccg
     7201 attggaagcg aaccacaccc tcgcaccggc agaaagcgct gctcgatttc gccgatgacg
     7261 tcgagaagaa cgcgcaggcg ctcgttgagg ccgagggccg caacaccggt aaacccaacc
     7321 acgtcacgat ggccgaagag attcctccga tggttgacca gatccggttc ttcgccggtg
     7381 cggcaaggat gttggagggc aagtcggccg gcgagtacat ggagaaccac acgtcgtgga
     7441 tccgtcgtga gccggtcgga gtcatcggcc aggtgtcgcc gtggaactac ccgatgatga
     7501 tggcgatctg gaagttctgt ccggccatcg ctgcgggcaa caccgtggtg atcaagccca
     7561 gtgacaccac accggtgaca acggtgatgc tcgccgagat cgcgtcccgg caccttccgc
     7621 cgggcgtact caatgtcgtt tgcggcgacc gcaatacggg tgccgcggtc gtcgcgcacc
     7681 ccacaccgca gatggtgtcg atcaccggct cggtggccgc gggccgcgcg gtggcggtga
     7741 gcgcgggcgg aaacttgaag cgcacgcatc tggagctggg cggcaaggca ccggtgatcg
     7801 tattcgacga cagcgacatc gcctcggccg cagaaggaat cgcgacggcg ggctacttca
     7861 atgcgggcca ggactgcacg gctgcgacgc gtgtgctggc gcgggcgtcg gtggcgtccg
     7921 atctgaccga tgcgctggcc gagcaggcca agggcgcgac gacgacgttc agcaaggcca
     7981 ccgatgacga ggacgcctgg gtgccgccgg tgaacaacat caaccagatg gagcgggtgc
     8041 tcagtttctt caccgacgtc ccatcgcacg ccaaggtggc gaccggcgga caacgccagg
     8101 gcgacaaggg tttctacgtc gagccgacgg tgatcggcgg cctgctgcag gacgaccggc
     8161 tggtccaaca ggagatcttc ggacccgtga tcacggtgca ggcgttctcc gacgaggacg
     8221 aggcggtcgc atgggccaac ggcacggaat acggccttgc ctcatcggtg tggaccaagg
     8281 acgtgtcgcg ggcactgcga gtgtcggcgg cgctggactt cgggtgcgtg tggatcaaca
     8341 cacacatccc gctggtcgcc gagatgccgc acggcggtta caagtcatcg ggtcacggca
     8401 aggacctgtc gatgtacggc ttcgacgact acacccgcat caagcacgtg atggcgtaca
     8461 cgggctaggg tttcaccgcg cgagcagacg caaagtgccc tatttcaccg gcgtgtcggg
     8521 tcactttgcg tctgctcggc cggactaccg gtgtgc
//
LOCUS       DASRJA010000044         3693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4189973,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000044 DASRJA010000000
VERSION     DASRJA010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4189973"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1118
                     /gene="mnmA"
                     /locus_tag="VFC01_01965"
     CDS             51..1118
                     /gene="mnmA"
                     /locus_tag="VFC01_01965"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZN78442.1"
                     /translation="MRVLVAMSGGVDSSVAAARMVDAGHDVVGVHLALSAAPGTLRTG
                     SRGCCSKEDAGDARRVADVLDIPFYVWDFADRFKDDVIDDFVSSYARGETPNPCVRCN
                     ERIKFSALAARALALGFDAVATGHYARLSEGRLRRAVDRDKDQSYVLGVLTAEQLRHA
                     VFPIGDTPKPQIREEAAHRGLAVAEKPDSHDICFIPSGDTRAFLGAKIGVRRGAVVDS
                     AGTVLAEHDGVHGFTIGQRKGLGIAGPGPDGQPRYVTAIDADSGTVHVGDAAELDVRT
                     LTGDSPVFTSGVAPHGPVECAVQVRAHGGIAEGVAELRDRKMVVDLRAPLRGVAPGQT
                     MVLYRSDPDGDEVIGSATITR"
     gene            complement(1190..1834)
                     /locus_tag="VFC01_01970"
     CDS             complement(1190..1834)
                     /locus_tag="VFC01_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HZN78443.1"
                     /translation="MPASAKPLVVWCAAIAMAVGCSHTVGGRALRATPGVDDESLSPV
                     DVETLMLDQSQMRAITGAGDDLTIIPTMDGKVPVDIEQLAKTTPAQCKWIFEETQTFG
                     PDVEEFHKTTYQDPPRGGLISEGAAAYRDPATARRAFDGLVARVDSCASTTFGPMFVG
                     EWSATPDSVQMRPGNGCGRDYRMKNVVLVEVTSCAFPGSVPDIVMTNILAKVPG"
     gene            1888..2898
                     /locus_tag="VFC01_01975"
     CDS             1888..2898
                     /locus_tag="VFC01_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HZN78444.1"
                     /translation="MNVFAAATGIGSWPGTSPHEAAEIVIGELHTLPHLVELPGRGVG
                     ADMIGRTGALLVDIGIDTVPRGYRIANGRSAVVRRAISLLDEDIDALEEAWEKAGLRG
                     GSRTVKVQAPGPITLAAQLELPGGHRAITDAGALRDLAGSLAEGMAAHRAQLQRRLDT
                     PVVVQFDEPSLPAALEGRLTGVTSLTPVHPVDESVAVGLLDDCVAIVGSEVAVHSCAP
                     GLPWNALQRSGLHAVSVDVSTLTPADLDGVGNFVDSGRTVLLGVLPTTAPTARPSAEE
                     IAKAAVAVTDRLGFARTVLRERIGITPACGLAGATPQWARTTIGLAQKAADAFAEDPD
                     AI"
     gene            2978..3472
                     /locus_tag="VFC01_01980"
     CDS             2978..3472
                     /locus_tag="VFC01_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN78445.1"
                     /translation="MSTDRIEKQVVLRAPLDRVWRAISDADEFGQWFGVRFDGPFVAG
                     KSVTGVITPTTVDEDVARAQEPHAGKSDSWQIIAVEPQRRLAFRWHPYAVEAGTDYSE
                     EPTTLVEFTLTETNDGVLLRIVESGFDKIPAERRTAAFEANSEGWAKQTELVQKYLAL
                     SETV"
     gene            3469..>3693
                     /locus_tag="VFC01_01985"
     CDS             3469..>3693
                     /locus_tag="VFC01_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN78446.1"
                     /translation="MSTAATAVAKAPVFDALGDPNRLRIVIRLCDGGPCSTSRVTQVI
                     PVSRQAATKHLLLLEAVGLVSSERQGRERIW"
BASE COUNT          619 a         1191 c         1262 g          621 t
ORIGIN      
        1 gtcgagcggg cgcgccaggc cgcgctggcc agcgcggggg ctgccgaaca atgagagtgc
       61 tggtcgctat gagtggcggt gtcgactcgt cggtcgctgc cgcaagaatg gttgacgcgg
      121 gtcacgacgt cgtcggtgtg cacctggcgt tgtcggctgc gccgggcacg ctgcgcaccg
      181 gatcgcgggg gtgttgctcg aaagaggatg ccggtgacgc tcgccgtgtc gccgatgttc
      241 tcgacatccc gttctatgta tgggatttcg ctgaccgctt caaggacgac gtgatcgacg
      301 acttcgtgtc gtcgtatgcc cgcggtgaga cgcccaaccc gtgcgtgcgc tgcaatgagc
      361 gaatcaagtt ctccgcgttg gccgctcgcg cgctggcgct cggcttcgat gccgtggcaa
      421 cgggccacta cgcgcggctg tccgagggtc gactgcgccg tgccgtcgac cgcgacaagg
      481 accagtcata tgtgctgggg gtgctgaccg ccgagcagtt gcgccacgcc gtcttcccga
      541 tcggcgacac acccaagccg cagatccgcg aggaagccgc tcatcgcggg ctggcggtgg
      601 ccgaaaaacc cgacagccac gacatctgct tcatcccgtc gggggacacc agggcgttcc
      661 tcggcgccaa gatcggcgtt cgacgcggcg ccgtcgtcga ctcagccggc acggtgttgg
      721 ccgaacacga tggcgtgcat ggctttacga ttggccagcg caagggcctc ggcatcgcgg
      781 gacctggccc tgacggccag ccgcgctatg tcaccgcgat cgacgccgac agcgggaccg
      841 tgcacgtcgg ggatgcggcc gagttggacg tccgaacgct caccggcgac agcccagtgt
      901 tcacctcggg tgttgcgccg cacggtccgg tggagtgcgc cgtgcaggtg cgtgcgcacg
      961 gaggcatcgc cgagggtgtg gccgaactgc gcgacaggaa gatggtggtc gatcttcgtg
     1021 ccccgctgcg tggcgtggcg cccgggcaga cgatggtgct ctaccgctcc gatcccgacg
     1081 gcgacgaggt catcggcagc gcgacgatca cgcgctagtt ctcgccgaac gttccttacc
     1141 gctcgaaaaa tcgccgaaat atcgagcggt aaggaacgtt cgcccagcct tacccaggca
     1201 ccttcgccag gatgttcgtc atcacgatgt caggcaccga acccggaaac gcgcacgacg
     1261 tgacctccac caggacgacg ttcttcatcc ggtagtcgcg gccgcacccg ttgcccggac
     1321 gcatctgcac cgaatccggt gtcgcgctcc actcgccgac aaacatcggc ccgaacgtgg
     1381 tggatgcaca gctgtcgacg cgtgcgacca ggccgtcgaa tgcgcggcgg gcggtggcgg
     1441 ggtcgcggta ggccgcggca ccctcggaga tcagaccccc gcggggtgga tcctgatacg
     1501 tcgtcttgtg aaactcctcg acatcgggac cgaaggtctg ggtctcttca aagatccatt
     1561 tacattgcgc tggagtggtt ttcgcgagct gctcgatgtc gacggggact ttgccgtcca
     1621 tggtggggat gatcgtcaga tcgtcccccg cacctgtgat tgcccgcatc tgcgactggt
     1681 caagcatcag cgtttcgaca tcgacgggtg acagggattc gtcgtcgacg cctggagtcg
     1741 cccgcagcgc gcggccgcca acggtgtgcg aacagccgac cgccatcgcg atggccgcgc
     1801 accagaccac caacggtttg gccgatgcgg gcatggcgtc gtccccttcc cggatcgaga
     1861 ctaaccggat cgaatattgt taaacgagtg aatgttttcg ccgcagcgac cggcatcggg
     1921 tcgtggccag ggacgtcgcc gcacgaagcc gccgagattg tgatcggtga actgcacacg
     1981 cttcctcatc tggtcgagtt gcccggccgc ggcgttggag ccgacatgat cggccgcacc
     2041 ggtgcgttgc tggtcgacat cggcatcgac acagtgccgc gcggctatcg aattgccaac
     2101 ggtcgcagcg ccgtggtgcg ccgggccatc agcctgctcg atgaggacat cgacgcgctc
     2161 gaagaggcat gggagaaggc cggtctacgc ggcgggtcgc gaaccgtgaa ggtgcaagca
     2221 cccggtccga tcacgctggc cgctcaactc gagctgcccg gcggccatcg tgcgatcacc
     2281 gacgccggcg cgctgcgcga tctggctgga tcgctggccg agggcatggc cgcccatcga
     2341 gctcagcttc agaggcgcct cgacacaccg gtggtggtgc agttcgacga gccgtcgctg
     2401 cccgccgcgc tggagggcag gttgacgggg gtcaccagcc tgacgccggt gcatcctgtc
     2461 gatgagtcgg tagcggtcgg cctgctggat gactgcgttg cgattgtcgg ctccgaggtc
     2521 gcggtgcaca gctgcgcgcc cggtctgcca tggaatgcct tgcagcgcag cggtcttcat
     2581 gcggtgtccg tcgacgtgtc gacgcttacg cccgctgatc tcgacggggt cgggaacttc
     2641 gtcgactccg gccgcacggt gctgctagga gtcctgccga cgaccgcacc gacggcgcgg
     2701 ccgtcggccg aagagatcgc caaggccgcc gtcgctgtca ccgaccgcct cgggttcgcg
     2761 cgcacagtgc tgcgtgagcg catcggcatt acgcccgcgt gtggactcgc tggcgccaca
     2821 ccgcagtggg cgcgcaccac catcgggctg gcacagaaag ccgccgacgc gttcgccgag
     2881 gaccccgacg cgatctagac gcttgcccag tacgcaacca aaaggttgcg cttcgcatcg
     2941 aacggcgcta gcgtgatagg caaccaggag gttgcacatg agtactgatc ggatcgaaaa
     3001 gcaagttgtt ctacgcgcgc cgctggaccg agtctggcgg gcgatcagcg acgccgacga
     3061 gttcggccag tggttcgggg tccggtttga cggcccgttc gtcgccggga aatcggtcac
     3121 cggcgtgatc acgccgacga ctgtcgacga agacgtagcg cgcgcccaag aaccccacgc
     3181 cggcaagtcg gacagctggc agatcatcgc ggtggagccg cagcgccggt tggcattccg
     3241 ctggcatcca tacgcggtcg aagcgggcac ggactactcc gaggagccca ccacactggt
     3301 ggaattcacg ctcaccgaga ccaacgacgg cgtgctgctg cgcatcgtcg aatccggttt
     3361 cgacaagatt ccggccgagc gtcggacggc ggcgttcgag gccaacagcg agggctgggc
     3421 caagcaaacc gaattggtgc agaagtacct cgccctcagc gagacagtgt gagcacagcc
     3481 gcgaccgccg tcgccaaggc gccagttttt gacgcgctcg gtgatcccaa tcggttgcgt
     3541 atcgtcatcc gcctgtgcga cggcgggccg tgctcgacct cgcgggtgac gcaggtgatc
     3601 ccggtgagcc gtcaggcggc caccaaacat ctgctgttgt tggaggcggt gggcctggtg
     3661 agcagcgaac gccaaggcag ggagcgaatc tgg
//
LOCUS       DASRJA010000045         4811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4189712,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000045 DASRJA010000000
VERSION     DASRJA010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4189712"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1733
                     /locus_tag="VFC01_01990"
     CDS             162..1733
                     /locus_tag="VFC01_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZN78447.1"
                     /translation="MSRPAISGLDTDRIPRVAAPRHRLPRPRGRVRAARRVLAALAAI
                     LVLTVTAVGWSGYRNLSGGITTSQALAGGPASVGGAQNILIMGLDSRLDQHGQPLPQD
                     IYDALHAGDETVGGYNANVLILLHLPGGNGPVSAVSVPRDDYVDMPGCPSGVCKGKIK
                     QAYGLAYQQTMESLSDNIAKAAREQTAREAGRKAEIDTVRRLLGVPIDHFIEVTLGAF
                     FQIAQVVQPITVCLNDDTSDSYSGADFHRGVQQIDAAQAMAFVRQRRDENDQLFTDLD
                     RTRRQQAFIVSLVTALRHGGALSSPNALRNLLQVARQNIAVDAGFDLAGFVDHAAALT
                     DSSLSLYTLRIREFGQDPQGEDVNMVDVQDIRTIVRHVFTDGSPPAPPSPTQSASTPT
                     TLDVVNATDHDGLGSAVEHSFGADGLNPGTVTTADKLAQTSTVAYGPGAGDAAQMLAD
                     RLQLTATASDAVAADTVRLTLGADFPAAQYMTDGTSTITATPVTTVDATASGTQAPAP
                     TDLTEMAGAKTPCVK"
     gene            complement(1734..3551)
                     /locus_tag="VFC01_01995"
     CDS             complement(1734..3551)
                     /locus_tag="VFC01_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HZN78448.1"
                     /translation="MTSACTVSPPPAPQSTDTSKTTLPPPPRATQIIMAIDSIGPGFN
                     SHLLSDQSPVNAAISSLVLPSSFRPIPDPASPTGSRWELDTTLLQSAEVTNQSPFTVT
                     YKIRPEASWTDNAPIAADDYWYLWRQMVSQPGVVDPAGYDLITGVQSVEGGKTAVVTF
                     SQPYPAWRELFNDILPAHIVKDIPGGFAAGLTRALPVTGGQFRVESIDPQRDEILLAR
                     NDRYWSAPAKPDQVLFRRGGSSAALADSIRNGDTQVAQVHGGAAAFAQLSAIPDVRTA
                     RIVTPRVMQLTLRAQQPALADSQVRKAILGLLDVDLLASVGAGDDNTVTLAQAQVRSP
                     SDPGYVPTAPPAMTKEGALTLLANSGYQLEPVETAPPTTPGTPAPENNRGRVTKDGVP
                     LTLVLGVAANDPTAVAVANTAADQLRNVGIAASVTALDPVALYGDALINNRVDAVVGW
                     HQAGGDLATSLASRYGCPALEATAVSTATPGSTPSPSPAPTSNTTPPSRPTTTATPTT
                     PSPAPEAGALIQAPSNITGICDRSIQPKIDAALDGTEDIGEVINAVEPRLWNMSTVLP
                     ILQDTTIVASGPSVQNVSLSGAVPVGIVGDAGKWTKTKQ"
     gene            4215..>4811
                     /gene="gjpA"
                     /locus_tag="VFC01_02000"
     CDS             4215..>4811
                     /gene="gjpA"
                     /locus_tag="VFC01_02000"
                     /inference="COORDINATES: protein motif:HMM:NF033942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane porin GjpA"
                     /protein_id="HZN78449.1"
                     /translation="MHVAARPHVTAGIALAGAAVIAASPMAPTLPDIHLPNEHEVQAA
                     AVRLTAGWDPLAAWQNAFNTASTNASTLADNFLLAPGVGLQQAIVNEVGFLKEVINDP
                     SSLGTVLQQIATNAQTVASGLTGVGASAATNKAAQAHSVDALHGALLSLLPTMLPAGV
                     DPATVTQVLDVLASPASGLLIGAVGPVISPGVALLNSAL"
BASE COUNT          802 a         1638 c         1584 g          787 t
ORIGIN      
        1 gcccgtccgc cgtactggct gctcatggtg ttcgccatcg ccgctttcgc ggcgcatggg
       61 ctgcggctat tgcgcgaaaa gcacgccgaa gccgagcccc gcaaaacctg agaaatccgc
      121 gccggccctc tcaggaggta ccgggataat ccggctctgt catgagtcga ccggccattt
      181 cgggtttgga tacggatcgg ataccgaggg tcgccgctcc gcggcaccgg ctgcctcgtc
      241 ctcgcggccg ggtccgtgcc gcccgtcgag tcctggctgc gttggccgcc atcctcgtgt
      301 tgaccgtcac ggcggtgggc tggtccggct accgcaacct gtccggcggc atcacgacgt
      361 cacaggcgct ggccggcggc ccggcgtcgg tcggcggtgc gcagaacatt ctgatcatgg
      421 gactggacag ccgactcgac cagcacggcc agccgctgcc ccaggacatc tacgacgcgc
      481 tgcatgccgg cgacgaaacc gtcgggggct acaacgccaa cgtgctgatc ctgctgcacc
      541 tgcccggcgg caacggcccc gtcagcgcgg tatcggtgcc ccgcgacgac tacgtcgata
      601 tgcccggatg tccgagcggc gtctgcaagg gcaagatcaa gcaggcctac ggactggcct
      661 accagcagac gatggaatcg ctgagtgaca acatcgccaa ggccgcccgc gagcagacgg
      721 cgcgcgaagc gggccgcaag gccgaaatcg acaccgtgcg acggctattg ggtgtaccga
      781 tcgaccactt catcgaagtc accctcgggg cgttctttca gatcgcgcaa gtcgtgcagc
      841 cgatcacggt gtgcctcaac gacgacacct ccgacagcta ctccggtgcg gacttccacc
      901 gcggcgtgca gcagatcgac gccgcgcagg cgatggcgtt cgtgcggcaa cgccgcgacg
      961 aaaacgatca gttgttcacc gatctcgacc gcacccggcg ccagcaggcg ttcatcgtgt
     1021 ccctggtgac cgcgctccgc cacggcggag cactgtccag cccgaacgcg ttgcgcaacc
     1081 tgctgcaggt cgcccggcag aacatcgccg tggacgccgg gttcgacctc gcgggcttcg
     1141 tcgaccacgc cgcagcgctg accgacagct cgctgtcgct gtacacgctg cggatccgag
     1201 aattcggcca ggaccctcag ggcgaagacg tcaatatggt tgacgtccaa gacattcgga
     1261 ccatcgtgcg ccatgtcttc accgacggat caccgccagc ccctccaagc cccacgcaat
     1321 ctgcttctac cccaacgaca ctcgacgtcg tcaacgccac cgaccacgac gggctcggat
     1381 ccgccgtcga gcattctttc ggcgccgacg ggctgaaccc cggcacggtc accaccgcag
     1441 acaagctggc ccagaccagc accgtcgcct atggacccgg tgccggcgac gccgcgcaaa
     1501 tgcttgccga tcgactccag ctcaccgcca ccgcctccga cgccgtcgcc gccgacacgg
     1561 tgcgactgac gctcggcgcg gatttccctg cggctcaata catgacggac ggcacctcga
     1621 ccatcaccgc gacgcccgtc acgacggtcg acgccacggc cagcggaact caggcgcccg
     1681 cgccgaccga tctcaccgaa atggccggtg cgaagacccc gtgcgtgaag tgatcattgc
     1741 ttggttttgg tccactttcc ggcgtcgccg acgatgccga ccggcacggc cccggacagg
     1801 ctgacgttct gcacgctcgg acccgacgcg acgatcgtgg tgtcctgcag gatcggcagc
     1861 acggtcgaca tgttccacaa ccggggctcg accgcgttga tcacttcgcc gatgtcttcg
     1921 gtgccgtcga gcgccgcgtc gatcttcggc tgaatgctgc ggtcgcagat gccggtgata
     1981 ttgctgggcg cctgaataag tgcacccgct tccggtgccg ggctcggcgt ggtcggcgtc
     2041 gctgtcgtgg tcggccgcga gggcggcgtc gtattgctgg tcggcgccgg agacggcgac
     2101 ggcgtcgacc ccggggttgc ggtcgatacc gccgtcgctt ccagtgcggg gcagccgtat
     2161 cgcgacgcca gcgaggtcgc caggtcaccg ccggcctgat gccagccgac gacggcatcg
     2221 acccggttgt tgatcaacgc atcgccgtag agggccaccg ggtccagcgc agtgactgag
     2281 gccgcgatgc cgacgttgcg cagttgatcg gcggcggtgt tggccacggc gaccgctgtc
     2341 ggatcgttgg ccgcgacccc gagcaccaat gtcagcggca cgccgtcctt ggtgacgcgg
     2401 ccgcggttgt tctccggcgc gggcgtgccc ggcgtcgtcg gcggcgcggt ctcgaccggc
     2461 tccagctgat agcccgaatt ggccaacagc gtgagcgcgc cctccttcgt catcgccggt
     2521 ggcgcggtcg ggacgtaacc gggatcggac ggggaccgca cctgagcctg cgcgagggta
     2581 acggtgttgt cgtcgcccgc ccccaccgac gcaagcaagt cgacgtcgag cagacccaga
     2641 atcgctttgc gcacttggga atccgcgagg gccggctgct gggcccgcag cgtcagctgc
     2701 atcacccgcg gcgtgacgat gcgcgcggtc cgcacgtcgg gtatcgcgga cagttgtgcg
     2761 aacgccgccg cgccgccgtg cacctgcgcg acctgggtgt cgccgttgcg gatcgaatcg
     2821 gccagcgcgg cagacgagcc gccgcggcgg aacagcacct gatcgggctt ggccggtgcg
     2881 ctccagtacc ggtcgttgcg ggccagcagg atctcgtcgc gctgcgggtc gatgctctcc
     2941 acccgaaact ggcccccggt gaccggcagc gcgcgtgtca accccgccgc gaacccgcca
     3001 ggtatgtcct tgacgatgtg ggccgggagg atgtcgttga acagctcgcg ccaggccgga
     3061 tacggctgcg agaaggtcac caccgcggtc ttgccaccct ccacggattg cactccggtg
     3121 atcaggtcgt agcccgctgg gtccacgacg ccgggttggc tgaccatctg ccgccagagg
     3181 taccagtagt cgtcggcggc gatgggggcg ttgtccgtcc aggacgcctc gggccggatc
     3241 ttgtaggtga cggtgaacgg gctctggttg gtcacctccg ccgactgcag cagcgtggtg
     3301 tcgagctccc aacgcgaccc ggtcggcgac gccgggtcgg gaatcggccg gaacgagctg
     3361 ggcaacacca gcgaactgat cgccgcgttc accggtgact gatcggacag cagatgcgaa
     3421 ttgaagccgg ggccgatgga gtcgatggcc atgatgatct gcgttgcccg cggtggcggc
     3481 gggagggtgg tcttcgacgt gtcggtgctc tggggtgcgg gcggagggct gacggtgcaa
     3541 gctgacgtca tcagcgtcag cgcgaatagt gcggcaacga cgcgatgggg gcccggcacg
     3601 ctgttcaggg tatcggcagg cccgaaagcc gtagcttcgg cgagggcaca aacgtttctg
     3661 ggctgccgga cgccgtagcg gtggccgggg tggccaccga atcgggacgc tgttgggaag
     3721 tggcagccga tgccacccgg tagcgctcct cagaggacgg cgaactgctc gcgtcggccc
     3781 caagagccca ctcgttaacc cgctgggtcc gaccggaatg aggcggacac tgcgtgctgc
     3841 accggtgctg gactgcccgg gatgccgggc gggacggcga acaccggcct cggtcgaact
     3901 cgccatcgcg ccgcagatca tcgccgccga gtcgccgagc cgggctaatc aaccacaagc
     3961 ccccgcaatt cttcaggggc ttgagattaa tagtagctac gtattgcgcg acgacggtcg
     4021 cgatcattcg ctgagctaat tatattccgt caagatggcc tatgaacagc gaccttcccc
     4081 ttggggaagg tcattgtgcc agctgttcac agcatctata gagcaactat acaaatttgg
     4141 gaaatgtata gatttccgaa cttactcgtg ggtaaattct tgccgtcatc gaatgttcga
     4201 gtaagggaga tcacatgcac gtcgccgcca ggcctcacgt cactgctggc atcgcactgg
     4261 ccggtgccgc cgtcatagcc gctagcccga tggcaccgac cttgcccgat atccacctcc
     4321 cgaacgaaca cgaggtgcag gccgccgcgg tgcggctcac ggcaggttgg gatcccctcg
     4381 cagcgtggca gaacgcgttc aacaccgcgt ccaccaacgc ctcgacgctc gccgataact
     4441 tcctcctggc cccgggcgtc gggctgcagc aggcgatcgt caacgaggtc ggtttcctca
     4501 aggaagtgat caacgacccg tcgagcctcg gcacggtgtt acagcagatc gccaccaacg
     4561 cgcagaccgt ggcgtctggt ctcacggggg tgggcgcgag cgccgccacg aacaaggcag
     4621 cacaggccca tagcgtcgat gccctccatg gcgccttgct cagcttgcta cccacaatgt
     4681 tgccggccgg tgtcgatccg gccacggtta cccaggtcct ggacgtcttg gcgtcgccag
     4741 cgagcggcct cctcatcggc gcggtgggcc ccgtcatcag ccccggggtg gcactcctca
     4801 acagcgcttt g
//
LOCUS       DASRJA010000046         6093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2496898,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000046 DASRJA010000000
VERSION     DASRJA010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496898"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFC01_02005"
     CDS             complement(<1..323)
                     /locus_tag="VFC01_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZN78450.1"
                     /translation="MRKLKWFSVIGACLAVTMTGCSFEGLNSLPLPGAVGRGPDAVTY
                     HVEIPNVSTMESNSPVMINDVVVGSVGKMTVRNWHADVEISVKPDVVVPANAVATVGQ
                     TSLLGS"
     gene            complement(329..1828)
                     /locus_tag="VFC01_02010"
     CDS             complement(329..1828)
                     /locus_tag="VFC01_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN78451.1"
                     /translation="MMASRNRLMAVTAVLLAVVLVVGAVVLVRQIFFGPHTITAYFPT
                     ATAIYPGDEVRVSGVKVGKIDSIQPEGTQTKMTLKVDHGVPIPANAKAVIVAQNLVAA
                     RYVQLTPAYRKGDGPTMHDGAVIPSNRTAVPVEWDEVKTQLMRLATELGPKTGVSGTS
                     ISRFIDSAANALDGNGDKLRQTLAQLSGAARIFAEGSGNIVDIIKNLQTFVTALRDSK
                     EQIVMFENRLASLTSVVNDSRSDLDAALSDLSVALGEVQRFVAGSRNQVSEQVGSLAD
                     VTQILVDNRMAFENILHITPNAVANYGNIYYPPSGGVSGSISLSNFSNPVYFVCGMIG
                     AIENTTAPETAKLCSQYLGPALRLLNFNNLPLPINLYLRPAPKPENIIYTDPKLAPGG
                     AGPGDPPEPFPSVSAYLGAGDIPPPPGWGQPPGPPGLYSPGNEVPATPSPALYPGAPI
                     PGPPTILPGPPPSVDGMLMPQSPALPADQAPPPVPPGTPLLPAEGGQRP"
     gene            complement(1812..2915)
                     /locus_tag="VFC01_02015"
     CDS             complement(1812..2915)
                     /locus_tag="VFC01_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN78452.1"
                     /translation="MQRYRGNELVKAGFIGVTVMILVIVAGLQPQRLLQYATAIKYQA
                     LFTEAGGLAPGNDVTVSGIKVGTVSSIKLDNGDALITFTIAGKAAVGSETTAHIRTGT
                     LLGERVLALESSGNGTLDHRQVIPVTRTSSPYSLTDAVSELTANTAATNTDTLNQSLD
                     TLAATLDQISPELGPTFDGLSRLSRSLNNRNESLAELLKTAGDVTGILSERSRQVNTL
                     ILNANDLLAVLNERRQAIVSLLANTSAVSRELTGVVADNEQELAPTLERLNRVNAMLV
                     KNRDNLSKAIPGLAKYELTQGEIVANGAYYNALIPNIQPAQILQPFLDYVFGFRRGVD
                     AGQPPDNAGPRAELPFPVNGIPLPGDLPNDGKP"
     gene            complement(2924..3952)
                     /locus_tag="VFC01_02020"
     CDS             complement(2924..3952)
                     /locus_tag="VFC01_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN78453.1"
                     /translation="MTRARGTFIKFTAFAVVMAILTAFLFLIFNQTRTGATNGYSAIF
                     TDASRLKTGDTVRVAGIRVGTVKAVSLQADRNVLVKFDADRDIVLTTATRAQIRYLNL
                     TGDRFLELADSPGSTKLLPAGSQIPMDRTAPALDLDLLLGGLKPVIRGLNPQDVNALT
                     SSLIQILQGQGGTLESLFSKTSSFTNSLADNGQVIEQMIDDLRTLLGTLSKDGDEFSG
                     AIDRLEQLVSGLSADRDPIGEAITSLDNGTASLADLLGRARPPLAGTIDQLNRLAPLL
                     DGDHKDYMDATLQRLPELYRKLARVGSYGAFFPYYICGITFRASDLTGRTVVFPWIKQ
                     ETGRCAEN"
     gene            complement(3949..5148)
                     /locus_tag="VFC01_02025"
     CDS             complement(3949..5148)
                     /locus_tag="VFC01_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN78454.1"
                     /translation="MNSSWVRPLTGLALIVAIGLIIALAIGLFEGSFTKTEPVTVISD
                     RAGLVMNNDAKVKMRGVQVGKVDSIEYRSDGTAALHLAMNPSQLHLIPSNVKVDIKSS
                     TVFGAKSVDMVSPDNPSPQPLRPGQVIQSQHVVVEINTVFQQLVQVLDKIDPTKLNQT
                     LGAIAKAFNGRGEKFGRTLTDFNAFLAKIEPSLPNLSHDLEAAAPTFSAYADAAPDLI
                     RTANSATTISNTIVDQQQELDQFLVSSIGLADIGNDVIGGNEPALAEVLRLLVPTTDL
                     LNRYHESLTCSLGGLAVQANSPPLPGNNSAVVLSAGLTLGTERYRYPNDLPKVAAKGR
                     PYCKELGLPNVPPEFRVPAVVADVGANPYQYGNQGILLNSAGLKNWLFGPIPGPPRNS
                     AQIGMPG"
     gene            complement(5158..6015)
                     /locus_tag="VFC01_02030"
     CDS             complement(5158..6015)
                     /locus_tag="VFC01_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN78455.1"
                     /translation="MGTRTVLRSRFPRLTRGLKKPTDLLGRIGDHMLFYLRALGGVPH
                     AAVHFRREIIRLIAEISMGAGTLAMIGGTVVIVGFLTLAAGGTLAVQGYSSLGDIGIE
                     ALTGFLAAFINVRISAPVVAGIGLAATFGAGVTAQLGAMRINEEIDALTAMAIRPVEY
                     LVSTRIVAGMIAITPLYSIAVVLSFVASQFTTVVLFGQSGGLYDHYFDTFLNPIDLLW
                     SFLQAVLMAITILLVHTYFGYFASGGPSGVGVAVGNAVRTSLVVVVSVTLLVSLSIYG
                     SNGNFNLSG"
BASE COUNT         1138 a         1812 c         1956 g         1187 t
ORIGIN      
        1 gatcccagca ggctggtctg tccgaccgtg gccaccgcat tggcaggcac gacaacgtcc
       61 ggcttgaccg agatctccac atccgcgtgc caattgcgga cggtcatctt gccgacgctg
      121 ccgacgacga cgtcattgat catgaccggc gaattcgact ccatcgtcga tacattcggg
      181 atctcgacgt ggtaggtgac ggcatctggc ccccgtccta ctgctccggg cagcggcagc
      241 gagttcaatc cttcgaacga gcaacccgtc atagtcaccg ccaggcatgc cccgatgaca
      301 ctgaaccact tcagttttcg catggcggtc acggtcgttg ccccccttct gccgggagca
      361 acggcgtacc gggcggaaca ggtggtggcg cctgatcagc cggaagcgcc ggggactgtg
      421 gcatcagcat gccgtcaacg ctcggcggcg gtcccggaag gatggtgggc ggcccgggaa
      481 tcggcgcgcc cgggtacaac gccggcgagg gagtggcggg tacttcgttg cccggcgagt
      541 acaaccccgg cgggccgggt ggctgtcccc acccgggggg cggcgggata tcgccggcgc
      601 cgaggtaggc cgacaccgat gggaaaggct cgggcgggtc acccggcccg gcgcctcctg
      661 gcgccagctt tggatcggtg tagatgatgt tttccggttt cggggcaggc ctcaggtaca
      721 agttgatagg taacggcagg ttgttgaaat tgagcaatcg cagagccgga ccgagatatt
      781 gcgagcacag tttcgccgtt tcgggcgcgg tggtgttctc aatggcgccg atcatgccgc
      841 agacgaagta aaccggattc gagaagttgg acagactgat tgatccgctt accccgccgg
      901 agggcgggta gtagatgttc ccgtagttgg cgaccgcgtt cggtgtgata tggaggatgt
      961 tctcgaaagc catgcggttg tcgaccagga tctgagtgac gtcggccagg cttccgacct
     1021 gttcggagac ttggttgcga ctgccggcga cgaagcgctg cacctcaccg agggcgaccg
     1081 acaaatcgga caacgctgca tccagatcgg acctgctgtc gttcacgacg ctggtcagac
     1141 tggccagccg gttctcgaac atgacaatct gttctttgct gtcacgaagg gcggtgacga
     1201 aggtctgcaa gttcttgatg atgtcgacga tgttgccgct gccttcagcg aaaatgcgtg
     1261 cggcgccgga caattgcgcc agtgtctgcc gtagcttgtc accgttgccg tccaatgcat
     1321 tcgctgcgct gtcgatgaac ctcgagatgg aagtgcccga cactcccgtc ttcgggccca
     1381 actccgtcgc caggcgcatc agctgggtct tgacctcgtc ccactcgacg gggacggcgg
     1441 ttcggttgct gggtatcacc gcgccgtcat gcatggtcgg tccgtcaccc ttccgatacg
     1501 ccggcgtgag ctgaacgtaa cgcgctgcaa ccaggttctg cgcgacgatt acggccttgg
     1561 cgttggccgg aatgggcacg ccgtgatcga ccttcaacgt catcttggtc tgcgtgcctt
     1621 cgggctggat ggagtcgatc ttgccgacct tgacacccga cacgcgcacc tcgtcgccgg
     1681 ggtaaatcgc tgtggcggtc gggaagtacg cggtgatggt gtggggcccg aagaagatct
     1741 gccgcaccag cactaccgct ccgaccacca gcacgactgc cagcagcacg gcggtgacgg
     1801 ccatgaggcg attacggctt gccatcattg ggcagatctc ctggtagcgg tattccgttg
     1861 accgggaagg gcagctcggc gcgtggccca gcgttgtccg gcggctggcc tgcatcgaca
     1921 ccgcggcgga acccgaagac gtagtccagg aacggttgca ggatctgcgc cggttggatg
     1981 ttgggtatca acgcgttgta ataggcgccg ttagccacga tctcgccctg ggtgagctcg
     2041 tatttggcca ggccggggat cgccttggac aggttgtccc ggttcttgac caacatcgcg
     2101 ttgacgcggt tgagcctctc cagtgtcggc gccagctcct gttcgttgtc ggcaaccacg
     2161 ccggtcagtt cccgagacac cgcagaggtg ttggcgagca ggctgacgat ggcctgccga
     2221 cgctcgttga gcaccgcgag cagatcattg gcattgagga tgagcgtgtt gacctgccgg
     2281 ctgcgttcgg acaggatgcc ggtcacgtcg ccggcggttt tcagcagctc ggccagactc
     2341 tcgttgcggt tattgagcga tcttgacagt cgggaaaggc cgtcgaacgt cgggcccagc
     2401 tccggcgaaa tctggtcgag cgtcgccgcc agcgtgtcca gcgactggtt cagcgtgtcg
     2461 gtgttggttg cagcggtatt cgcggtgagt tcactgacgg cgtcggtcaa ggagtacggc
     2521 gaggacgtac gcgtcaccgg aatgacctga cggtggtcga gcgtgccatt tcctgaggac
     2581 tccagggcca gcacacgctc gccgagcaac gtgcccgtgc ggatgtgcgc tgtggtttcc
     2641 gagccgactg cggccttacc cgcgatggtg aaagtgatca gcgcgtcgcc gttgtcgagt
     2701 ttgatcgacg acacagtgcc gaccttgata cccgataccg tcacatcgtt gccaggggcg
     2761 aggccgccgg cctcggtgaa gagcgcctgg tatttgatgg ccgtggcgta ttgcagcaac
     2821 cgttgcggtt gaagcccggc cacgattacc aggatcatca cagtgacgcc gatgaagccc
     2881 gccttgacga gttcgtttcc gcgatatctc tgcatcaatg ccattagttt tccgcgcacc
     2941 tcccggtctc ttgcttgatc caggggaaga ccacggtgcg gcccgtcagg tcgctggctc
     3001 ggaaggtgat cccgcagatg tagtagggga agaacgcgcc gtaggaaccg acccgcgcga
     3061 gtttgcggta gagctcgggc aggcgctgaa gggtggcgtc catgtaatct ttgtggtctc
     3121 catccagcag gggcgccagc ctgttcaact ggtcgatagt gccggccagc ggcggacgcg
     3181 cccgaccgag caggtcggcc agcgaggcgg tgccgttatc gagggacgtg atcgcctcgc
     3241 cgatcggatc gcggtcggcc gacaagccac tgaccagttg ctcgagccgg tcgatcgccc
     3301 cagagaattc atcaccgtcc ttggacagcg tccctaacag cgttcgcaag tcgtcgatca
     3361 tctgctcaat gacctggccg ttgtccgcca acgagttggt aaatgacgat gtcttcgaga
     3421 acagcgattc gagagtcccc ccttggccct gcaggatttg gatcagcgac gacgtgagcg
     3481 cgttcacgtc ttgcggattg aggcctcgga taacgggttt caggccgccg agtagcagat
     3541 cgaggtccag agcgggtgcc gtgcggtcca taggtatctg cgagcccgcc ggtaatagct
     3601 ttgtcgatcc cgggctgtcg gcgagttcca gaaagcgatc gccggttagg ttgagatagc
     3661 gaatctgcgc cctggtcgcc gtggtaagca cgatgtcacg atccgcgtcg aatttgacga
     3721 gcacgttgcg gtccgcttgc agcgataccg ccttcacggt gccgacccga attcccgcga
     3781 cccgcacagt atcgcccgtt ttgagtcgtg aagcgtcggt gaaaatcgcc gagtatccgt
     3841 tcgttgcacc ggttctcgtt tggttgaaga tcaagaacag gaaggcggtc aggatcgcca
     3901 tcacgaccgc gaaggccgtg aacttgatga acgtgcccct cgcgcgtgtc atcctggcat
     3961 tccgatctgt gctgagttgc gcggcggtcc agggatcggc ccgaacaacc agttcttgag
     4021 tccggcggag ttcagcagga tgccctgatt tccgtactgg tatgggttgg cgccgacatc
     4081 agcgacgacg gccggcaccc ggaactcggg tggaacgttg ggcaacccta gttccttgca
     4141 gtagggacgg cccttggcgg cgaccttcgg caggtcgttc ggatagcgat agcgttcggt
     4201 accgagcgtc aggccggcag acagcaccac ggcgctattg ttgccaggaa gtggtggcga
     4261 attcgcctgc acggcaagtc cgccaagtga gcaagtcaac gactcgtgat accggttcag
     4321 caggtcggtg gtgggcacga gcagacgcaa cacctcggcc agcgccggtt cgttgccgcc
     4381 gatcacgtcg tttccgatat ccgccaaacc gatggagctc accaagaatt ggtcaagctc
     4441 ctgttgctgg tcgacgatgg tattgctgat cgttgtggcg ctgttggctg tccggatgag
     4501 gtcgggcgct gcgtccgcgt acgcactgaa cgtcggagcc gcggcttcga ggtcgtggct
     4561 gagattcggc aggctcggtt cgatcttggc caggaacgcg ttgaagtcgg tcagcgtcct
     4621 gccgaatttc tcaccccggc cgttgaacgc cttcgcgatc gcgcccaggg tctgattgag
     4681 tttggtgggg tcgatcttgt cgaggacctg caccaactgc tgaaagacgg tgttgatttc
     4741 gaccacgacg tgctgactct ggatcacctg accgggccgg agcggctgcg gtgaaggatt
     4801 atccggcgaa accatgtcga cagacttggc gccgaacacc gttgacgact tgatatcgac
     4861 cttcacgttc gacggaatga gatgcagctg cgacggattc atcgccaggt gcagtgccgc
     4921 cgtgccgtcg gaccggtact cgatcgaatc gactttaccg acctgcacgc cacgcatctt
     4981 caccttggcg tcgttgttca tcaccagacc ggctcgatcc gaaatgaccg tcactggttc
     5041 ggttttggtg aagctgcctt cgaacaggcc aatggccaag gcgatgatca agccgatggc
     5101 gacaatcagc gccaagccgg tcagcgggcg gacccaacta ctattcatcg gcaccaccta
     5161 tcccgacaga ttgaagttgc cgttggagcc gtagatggac agcgacacca gaagggtgac
     5221 cgatacaacc acgacaaggg aggtgcgcac cgcgttccca acggcgaccc cgacacctga
     5281 aggtccgccg gaggcgaaat atccgaagta agtgtgcacc aacaagatcg tgattgccat
     5341 cagtaccgcc tgcaggaatg accacaatag atcgatcggg ttgaggaaag tgtcgaaata
     5401 gtggtcatag agtccacccg actgtccgaa caacaccacc gtcgtgaact gggaagctac
     5461 aaacgacagg accaccgcga tcgagtacag cggggtgatt gcgatcattc ccgcgacgat
     5521 cctggtggat accaggtatt cgacgggacg gatcgccatc gccgtgagcg cgtcgatctc
     5581 ctcgttgatg cgcatcgccc ccaactgcgc cgtcacaccc gcgccgaagg tcgcggccaa
     5641 cccgatgccg gccaccaccg gggcagagat gcgcacgtta atgaacgccg ccaagaagcc
     5701 cgtcagggct tcgatgccga tgtcgcccag ggagctgtac ccctgaaccg ccagcgttcc
     5761 gccagcggca agggtcagga agccgacgat aacgacggtg ccgccgatca tcgccaaagt
     5821 tcctgcgccc atggagattt cggcgatcag ccggatgatc tcacgacgga aatgaacggc
     5881 cgcgtgcggc acgccgccca gggctcgcaa gtagaacaac atgtgatcgc cgatgcggcc
     5941 cagcaggtcg gtcggcttct tgagccctcg ggtcagccgc ggaaagcgac tgcgcagtac
     6001 tgttcgtgtt cccatcgctt agtggcttcc cgtcgtcatc tggataccga tggccgtcac
     6061 gaccacgttg atcacgaaca gcgccatgaa cgc
//
LOCUS       DASRJA010000047         5967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2205569,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000047 DASRJA010000000
VERSION     DASRJA010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2205569"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VFC01_02035"
     CDS             <1..1152
                     /locus_tag="VFC01_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN78456.1"
                     /translation="GGWQSFDALMDRARAAYAHPRNASGDPMMIYFTSGTTGYPKMVL
                     HTHASYPIGHVITGRYWLDNGPDDLHWTISDTGWAQAAWTCFFAPWNMGAAVFVWDAR
                     GKKFDAQETLAMFERFPITTFFAPPTAYRMLVQEPLRAHRFPTLRHCMGAGEAVNPEV
                     IEAWREGTGHHIYEGYGQTETVLLAATFPATPWKPGSMGPTAPGHVLGIIDDDGHEVR
                     RGTEGDLAVRVAPHRPVGMFAGYWRNEDATRRCHRGDWYVTGDRASMDEDGYLWFVGR
                     ADDVINSASYRIGPFEVESALVEHPAVAEAAVVGKPDALRGEIVKAFVVLTAGHTPSP
                     ALAAELQEHVKQVTAPYKYPREVEFVADLPKTISGKIRRTELRQRERGR"
     gene            1369..5550
                     /locus_tag="VFC01_02040"
     CDS             1369..5550
                     /locus_tag="VFC01_02040"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HZN78457.1"
                     /translation="MRPSIFSSRSQFCAVVLILAAALSLGLATPALAQFVQGDLTGNW
                     TMHGLTTANFEQDPGPANWFRGSFTLSSTGVVLSGAIELADGSTRVLIGGKLTIGPTG
                     LVGGTLNVAGSVVNVRATMTPDKNGVVGVADDAAHARDEVFALVRQTATTFSTTELMG
                     QWIVYGISTPEDGLAAAAIARGALTFNELGALQATEQQPALLNTRGGDITDGVDQLTG
                     GELTVSSAGVVAGSLNGNTGGPTPEDITYTISGVIEAGGDVIVGVATREVVATGAKGR
                     IFFVAMKSGAAPLAIAAAPAVIGDVAGTWRSYIHIVINDDPRFGLWLEGLVVIRGTDG
                     FVSGTLRNADGTTGALAGTLTTRPDGDPATRLSGDLLLRLSVPGFVPFVLPAKFQGGV
                     SPAHDRIFSSIFINVAVPPASLTIHGLAMLVHQPVSTVQFAAPTYTVREQIGGSTALI
                     SVTRSGTVGVVSVDWATVPGGTATPGVDYTAATGTVTFPNGVATVTFPIAIADDNVLE
                     PNKTVNLVLRDPTNGAVIGPRSTAQLIIQDDEQIVQFHAASANITVNEGVPSVTLTLT
                     RTGPSATAFTVPVVIDGGTAVSNVDYPASLDSGLVVAFAPGQLSKTVSVPLLDDVLLD
                     GVKTLVLRLGTPSSPNVLLGVQQTSTITIVDNESGSVRFAVAASSIVEGGLAKITVIR
                     TGTNLQAGIDITYTVTGGTATSDDYTVCLALPTPAAPCQAVVGTGTLSFARGQTTQTI
                     FVQTTADTLNEPSETVIIQLGNAQNLAVLTAPTTHTLTILDNDRPGVLRFTTSALTVA
                     EPQGADRTVQLTVTRAVTLPAGVNFASGVTVDYAVTAGNATSGEDFTLLGTGTLTFDA
                     FQASQTISLVIHPDDVPEGTETLTVTLSNPTGGATLGTPSAITITITDDERAVFFASD
                     RVTVVEATLSVAITLLRSGPPAGGFTIPINIAPASTAVAGQDYPGTFTGATARFASNQ
                     MSAVVVIPLLNDAFLDGSKELVLELGTPVPIAPAPDAPAVGTRRLMTIALGDNDTPGE
                     IGFVALASTVNEGAKLRITVGRRGVNLARDVTVKYRVATTPAPTATEGTDFQFVDAAG
                     TPLPGTPPITGTLTFGPGATSAFIDVKALLDGIKEPTESFTIELFENSHGALINGRTR
                     HVVTINDTNQAGVIQWSPAVVTANEESGAVTLTIIRTGTNLSQDVSVSYRLDAPNAGT
                     ATPSADYSSPLGTVTFAAGQTTQTVTLTPFNDNLTEPTETIRILLHDPTGGATLGLNK
                     VVIVNLVDGAERFNGQYSGTFTGSFEFGGPVSGTERFSVSNAVITVSQICGGGVCDNA
                     ITNSTGDVFFNGDVTFTFGSLVVGDSTCAFTGRFTPSGTAVSAAGTWTCSSVEDGQGN
                     GTWRATRTSLVVPAP"
     gene            5601..>5967
                     /locus_tag="VFC01_02045"
     CDS             5601..>5967
                     /locus_tag="VFC01_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN78458.1"
                     /translation="MTRRPFTSEHEMLRKTVRGFVEREVTPHVGAWEEAGQIPKSFWR
                     RLGELGFLGLDFPVEYGGAGGDFFSSVVLGEEMARCRSGGVAFSVLVHTDMSSPWLVR
                     FGTEAQKRKYLPGIIRGETV"
BASE COUNT         1001 a         2017 c         1958 g          991 t
ORIGIN      
        1 ggcggctggc agagcttcga cgcgttgatg gaccgcgcgc gagctgccta cgcccatccc
       61 cgcaacgcca gcggcgatcc gatgatgatc tacttcacct cgggcaccac gggatatccc
      121 aagatggtcc tccacacgca cgcctcgtac ccgatcggcc acgtcatcac cggccgctac
      181 tggctcgaca acggccccga cgaccttcac tggaccatct cggacaccgg ctgggcgcag
      241 gcggcgtgga cctgcttctt cgcgccgtgg aatatgggcg ccgccgtctt cgtctgggac
      301 gcgcgcggca agaagttcga cgcgcaggaa acgctggcga tgttcgagcg ctttccgatc
      361 acgacgttct tcgcgccgcc cacggcctac cgcatgctcg tgcaggagcc gctgcgcgcg
      421 caccgcttcc ccacgctgcg tcactgcatg ggtgccggcg aagcggtcaa tcccgaggtg
      481 atcgaggcat ggcgcgaagg caccggccac cacatttacg agggctacgg ccagacggag
      541 acggtcttgc tggccgcgac gtttccggcg acgccgtgga agcccggctc gatgggtccc
      601 actgcgccgg ggcacgtgct cggcatcatc gacgacgacg gccacgaagt gcggcggggc
      661 actgagggcg atctggccgt gagggtggca ccgcaccgcc cggtcggaat gttcgccggc
      721 tactggcgca acgaggacgc gacgcgacgc tgtcaccgcg gcgactggta cgtcacaggc
      781 gatcgtgcat cgatggacga ggacggctat ctgtggttcg tcggtcgcgc ggacgatgtg
      841 atcaacagcg cctcgtatcg catcggtccg ttcgaggtcg agtcggcgct cgtcgagcat
      901 ccggcggtgg ccgaagccgc ggtcgttggc aagccggatg cgctccgcgg cgagatcgtg
      961 aaggcgttcg tcgtgctgac ggccggccac acaccttcgc ccgcgctcgc cgccgagctg
     1021 caagagcatg tcaagcaggt gaccgcgccc tacaagtatc cgcgcgaggt ggagttcgtc
     1081 gccgacctgc cgaagacgat ttcgggaaag atccggcgta ccgagctgcg acagcgcgag
     1141 cgcgggcgct aacgtctcgg gagatcgacg ttcaacgacc cttactgaag gcgaacctcg
     1201 ccagccactg aggcagtctg ccgtggtatc gcccgcggcg ggtgtaggtt ttgcgacacc
     1261 taaaattctg ctagctttcg cgtcgcatgc tgcctagaat gccggcctaa ttgctgtgct
     1321 ttgtagcacc cgcgattgga gcggggtcat gagacctcgg gggcatttgt gcgaccgtct
     1381 attttttcct cgcgttctca attctgtgcc gtcgttctca tcctcgctgc ggcgctgtcg
     1441 ctcggtctcg ccacgcccgc gctcgcgcag ttcgttcaag gcgacctgac gggcaactgg
     1501 acgatgcacg ggctcaccac agcgaatttc gagcaggatc ccgggcccgc caactggttt
     1561 cgcggatcgt tcacgctgag cagtaccggc gtagtgctct ccggcgccat agagctcgcg
     1621 gacggcagca cgcgcgtgtt gatcggcggc aagctcacca tcggcccgac cggtctcgtc
     1681 ggcgggacgc tgaacgtggc cggctccgtg gtcaacgtac gcgcgacgat gacgcccgac
     1741 aaaaacggcg tcgtcggagt cgcggacgac gccgcgcacg cgcgcgacga ggttttcgcg
     1801 ctcgtgcggc agaccgccac gacgttttcc acaacggagc tgatgggcca gtggatcgtc
     1861 tacggcatca gcacgcccga agacggcctg gccgctgccg ccattgcacg cggcgcattg
     1921 acgttcaatg agctcggcgc cctgcaggca accgagcagc agcccgcgct gctgaacacc
     1981 cgtggtggcg atatcacgga tggcgtcgac cagctcacag gtggcgagct caccgtttcg
     2041 agtgccgggg tcgttgcggg ctcgctcaac ggcaacacgg gcggaccgac acccgaggac
     2101 atcacgtata cgatcagcgg cgtgatcgag gccggcgggg acgtgatcgt cggcgtcgcc
     2161 acgcgcgagg tcgtggcgac gggcgcgaag ggtcggatct tcttcgtcgc gatgaagtcg
     2221 ggcgcggctc cactcgcgat cgcggcggct ccggccgtga tcggcgatgt cgccgggacg
     2281 tggcgcagct acatccatat cgtcatcaat gatgacccgc ggttcggtct ctggctcgag
     2341 ggcctggttg tcatccgcgg gacggacggc ttcgtgtccg gcacgcttcg caatgccgac
     2401 ggcacgacgg gagccctggc cggtacgctc accacccgcc ccgacggcga tccggcgacg
     2461 aggctctcgg gcgacctcct gttacgcttg tcggttccgg gcttcgtgcc gttcgtgctg
     2521 cccgcgaagt ttcagggcgg cgtgtcaccg gcacacgacc ggatcttcag ctcgatcttc
     2581 atcaatgtcg ccgtgccccc cgccagcctg accatccatg gcctggcgat gctcgtgcac
     2641 cagccggtct ccaccgtaca gttcgccgcc ccgacctaca ccgtgcgcga gcagatcggc
     2701 ggctccaccg cgctgatcag cgtcacgcgc agcggtacgg tcggcgtcgt cagcgtggac
     2761 tgggcgacgg ttccgggcgg cactgcgacg ccgggcgtcg actacacggc agccacgggg
     2821 accgtcacgt tcccgaatgg tgtggccacc gttaccttcc cgatcgccat cgccgacgac
     2881 aatgtgttgg agcccaataa aacggtcaac ctcgtcttgc gtgatccgac gaatggcgcg
     2941 gtgatcgggc cgcggtcgac ggcccagctg atcattcagg acgacgagca gatcgttcag
     3001 ttccacgcgg cgtcggccaa tatcaccgtg aacgagggcg tgccctcggt cacgctcacg
     3061 ctcacccgta ccgggccgtc agcaacggcc ttcaccgtgc cggtggtcat cgacgggggc
     3121 acggccgtct ccaatgtcga ttatccggcg agcttggatt ccggcctggt cgtggcgttc
     3181 gccccgggtc agctgagcaa gacggtcagt gtgccgctgc tcgacgatgt gctgctcgac
     3241 ggcgtgaaga cgctcgtgct gaggctcggc acgccgagct caccgaacgt tctcctcgga
     3301 gtccagcaga cgtcgacgat cacgatcgtc gacaacgaat cgggctccgt gaggttcgcc
     3361 gtggccgcgt cctcgatcgt cgagggcggc ctcgccaaga tcaccgtcat acggacgggc
     3421 acgaatctac aagccggcat cgacatcacc tacacggtga ccggcggcac ggcgacatca
     3481 gacgactaca cagtctgcct cgcgctgcca accccagccg cgccgtgtca ggcagtcgtc
     3541 ggcacgggca cgctgtcgtt cgcgcgtggc cagacgacgc agacgatttt cgtgcagacg
     3601 acagcggaca cgctgaacga gccgtccgag accgtgatca ttcagctggg taacgcacag
     3661 aacctcgcgg ttctcaccgc gccgacgacg cacacgctga cgatcctcga caacgacagg
     3721 cctggagtgc tgcgcttcac gacgtcggcg ctgaccgtgg ccgagccgca gggcgcggat
     3781 cgcacggtgc agctgaccgt gacgcgagcc gtaacgctgc ccgccggggt gaacttcgcc
     3841 agcggtgtga cggtcgacta cgcggtcacc gcgggcaacg cgaccagcgg tgaggacttc
     3901 acgcttctcg ggactggcac gctgacgttc gacgcgttcc aggcgtcgca gacgatcagt
     3961 ctcgtgatac atcccgacga cgtccctgag ggcacggaga ccctgaccgt gaccctgagc
     4021 aatcccacag gcggcgccac gctcggcact cccagcgcga tcacgatcac gatcacggac
     4081 gatgagcgcg ccgtcttttt cgcctcggac cgagtgacgg tcgtcgaggc cacgttgtca
     4141 gtggcaatca cgctgttgcg ctccggtccg ccggccggcg gtttcaccat tcccatcaac
     4201 atcgcgccag ccagcaccgc ggttgcagga caggactatc cagggacctt caccggcgcc
     4261 accgcgcgat ttgcctcgaa ccagatgtcc gccgtcgtcg tgattcctct gctcaatgac
     4321 gcgttcctcg acggctcgaa ggagctggtg ctggagctcg gcacgccggt ccccatcgct
     4381 ccagcgccgg atgcgccggc cgttggaacc cgacgcctca tgacgattgc gctgggtgac
     4441 aacgacactc cgggcgagat tggcttcgtc gctctcgcct cgacggtgaa cgaaggcgcc
     4501 aagctgcgaa tcaccgtcgg ccgccggggt gtgaacctcg cgcgtgacgt gacggtgaag
     4561 taccgggtcg cgacgacacc ggcgccgacg gcgaccgagg gcaccgactt ccagtttgtc
     4621 gatgccgccg gcactcccct tcccggcacg ccgccgatca ccggcacatt gacgttcggc
     4681 cccggcgcca ccagcgcgtt catcgacgtc aaggcgctgc tcgacgggat caaggaaccg
     4741 acggagagct tcacgatcga gctgttcgaa aactcgcacg gggcgctgat caacggccgc
     4801 acgcgccacg tggtgaccat caacgacacg aaccaggcgg gtgtgatcca gtggtcgccc
     4861 gcggtggtga ccgccaacga ggaaagcggc gccgtgacgc tgacgatcat acggaccgga
     4921 accaacctct cgcaggacgt cagcgtgtcg taccggctcg acgcccccaa cgccggaacg
     4981 gcaacgccga gcgctgacta cagctcgccc ctgggaacgg tcacgttcgc ggcgggccag
     5041 acgacgcaga cggtgacgct gacgccgttc aacgacaacc tcacggagcc gaccgagacc
     5101 attcggatcc tgctccacga ccccacgggc ggagcaacgc tcggactcaa caaggtcgtg
     5161 atcgtgaacc tcgtggacgg cgctgagcgc ttcaacggcc aatattccgg aacgttcacg
     5221 gggtcgttcg agttcggcgg gccggtgtcg ggcaccgaac gcttcagcgt cagcaacgcg
     5281 gtcatcaccg tgtcgcagat ctgcggcgga ggcgtgtgcg acaacgcgat cactaacagc
     5341 actggcgacg tcttcttcaa cggggacgtc acgtttacgt ttggctcttt ggtggtcggc
     5401 gactcgacct gcgcctttac cggccggttc accccgtccg ggacagcggt gtcggcggcc
     5461 ggaacctgga cctgcagctc ggtcgaggac ggtcagggca acgggacgtg gcgggcgacg
     5521 cggacttcgc tggtggtgcc cgcaccgtaa ctgtcgcctg gctgggctat gatcgagccc
     5581 ctctggaaca aggaggggcc atgacgcgcc ggccgttcac gtccgagcac gagatgctgc
     5641 gcaagaccgt gcgcggcttc gtcgagcgcg aggtcacgcc gcacgtcggc gcgtgggaag
     5701 aggccgggca gatccccaag tcgttctggc gacgcctggg cgagctcggc tttctcgggc
     5761 tcgactttcc cgtcgagtac ggcggcgccg gcggcgattt cttttcgagc gtcgtcctcg
     5821 gcgaggaaat ggcgcgctgc cgctcgggcg gcgtcgcctt cagcgtgctc gtgcacacgg
     5881 acatgtcgtc tccctggctc gtgcgcttcg gcaccgaggc ccagaaacga aaatacctgc
     5941 cgggcatcat ccgcggcgag accgtgt
//
LOCUS       DASRJA010000048         6044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_238179,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000048 DASRJA010000000
VERSION     DASRJA010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238179"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VFC01_02050"
     CDS             <1..1151
                     /locus_tag="VFC01_02050"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZN78459.1"
                     /translation="VGTLDRGSIGHAALANGIAAHVLDFDDTFNPERTTIHGSAPLWP
                     AIAAAGELGSVSGRLAVEAFVAGFEVQTRVAIAAGPGHYDVGWHVTGTVGHIGAAVAT
                     ARLLGLAADQTLAALGTGATQSAGMKVVYGSMGKSLHAGKAAVDGLLSGFLARDGFTS
                     ATEPIEGHRGFLHLFSPDPVAERAVEGLGAVWYLPRDGFKPYACGSLTHPPAQALLEL
                     RSEHGLTADDVAAVDAYVHDYVKTTTGLAEPRTGLEGKFSIYHVLAVALADGAALLDQ
                     FTDERVADLELAQLRKQVHVHTDPAQTKDSARVVVTLHDGRTLERHVAHNLGTPDNPM
                     TDQQLEDKFVGLASPVLGQGRTEELATTCWRLLDLDDIRALLALTVPG"
     gene            complement(1148..2050)
                     /locus_tag="VFC01_02055"
     CDS             complement(1148..2050)
                     /locus_tag="VFC01_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN78460.1"
                     /translation="MQPTHFHPDLRRVARYTPKQIVTPVTVPFIRAAERLMWHRPPKD
                     IEVLTLPSGVGIRLFRPTGVTGQAPGLLWIHGGGYVIGRAAQDDVLCRRYARELGVTV
                     ASVEYRLAPENPYPASLEDCYSALQWLVRLPSVDPARVAIGGASAGGGLAAALALLTR
                     DRGEISLAAQLLVYPMLDDRTVGRRDLENPGHRLWNQSSNKFGWSAYLGGADPQVAVP
                     ARREDLSGLPPAWLGVGTFDLFHDEDLAYAERLKAAGVPCEVEVVAGAFHGFDGIVPK
                     ADVSQSFFNSQCAMLREAFAPAAA"
     gene            complement(2068..4230)
                     /locus_tag="VFC01_02060"
     CDS             complement(2068..4230)
                     /locus_tag="VFC01_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN78461.1"
                     /translation="MSKSALAITEEHTDLADSAIGQLNRLNSRAAARATLENGATHPS
                     EIWSAGKDLGWNGLAIAEDHGGSGFGLAELAVVLEAQGRELSPGPFLPSVAAAVVIDR
                     CAPDSVRAQLLPALADGSAVGALALAGNVTIGSDLVVTGESPAVLGAPDADVLVIAAG
                     EDVVIVDAKADGVTITALESLDTTRSVGTVALRGVTVERDRVLRGAARKALTVFRILA
                     SAEAVGVSWAALDMAVEYAKVREQFGRTIGTFQAVKHHAANMLVNAEETTAATWDAAR
                     ADDLDSAWFPAAVAAAHAIRTQIFNAQYNIQLHGGIAYTWEHDAHLYLRRARTLAALM
                     EEGGDPLLDIVEGQRSGQAHGASFTLPAEAEAHRDQAREAVAHLQSLPQDKQRDYLVE
                     SGYLVPHWPKPWGRAADVLEQLVIEEEFAGIERPDMGITGWVALTIAQAGTDDQRERW
                     VEPVLRGQVMWCQLFSEPGAGSDAAAVRTAAKKVDGGWRVTGQKVWTSLAQYCQWGLA
                     TVRTDPDAPKHAGVTMMAIDMKAPGVTVNPLRGITGDSHFNEVFFDDVFVPDEDVVGD
                     VNKGWLVARATLGNERISIGGGSGGAVGFSPDHLIKLLDASPSAANYARRAGECIAET
                     HTLRLLNLRRASRAIAGAEPGPEGNVTKLLVAEAGQRITELGMELSGSAAVVGQAPKL
                     LRSYLGNRAMTIAGGTSEITRNTIAERILGLPRDPLLK"
     gene            4404..5627
                     /locus_tag="VFC01_02065"
     CDS             4404..5627
                     /locus_tag="VFC01_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN78462.1"
                     /translation="MTVRPDTRYPGPTIWTRARWFLKAGPADYMLAMSVASASLPVIG
                     KHLEPLGAATAMGVWGYRHMPDFLGATARSWFEPGNAEVRKLERDSTHNVVEAALRGV
                     VRAEDLDIEWPAPESAPPLWKFRQHRRSVYRTSVRYGNLPSQLLDVWRRKDLPAEPAP
                     VLIFVPGGAWVHGSRLLQGYALMSHLAEMGWVCLSIDYRVAPHHRWPAHLTDVKTAIA
                     WARANVDKFGGDRNFVAVAGCSAGGHLSALAGLTANDPEMQCELPDGSDTSVDAVVGI
                     YGRYDWEDRSTAERARFVDFLERVVVGRKIAKHPDVFRKASPIARVHKDAPPFLVIHG
                     TGDSVIPVEQARSFVAKLRAASHSVVSYVELPGAGHAFDMIDGARTGSMATAIGLFLN
                     QIHRNRSLTRAKQVI"
     gene            5654..>6044
                     /locus_tag="VFC01_02070"
     CDS             5654..>6044
                     /locus_tag="VFC01_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HZN78463.1"
                     /translation="MKRLRGWDAVLLYSETPNVHMHTLKLAVIQLDDLGGRTFGIDEF
                     RKVIHGRLHKLDPFRYQLIEVPGKIHHPMWRENCEVDLDYHVRPYRVDAPGGRRQLDE
                     AVGEIASTMSLIVTPRCLPVALMSASAS"
BASE COUNT          983 a         2049 c         2026 g          986 t
ORIGIN      
        1 tcgtcggcac cctcgaccgc ggctcgatcg gccacgcggc gctggccaac ggcatcgccg
       61 cccacgtgct ggacttcgac gacacgttca accccgagcg gaccaccatc cacggcagcg
      121 cgccgctgtg gccggcgatc gccgcggcgg gcgagctggg ttccgtttcc ggccggctcg
      181 ctgtcgaagc gttcgtcgcg ggcttcgagg tgcagacccg cgtcgcgatc gcagcggggc
      241 cggggcacta cgacgtcggc tggcatgtga ccggcacggt cggacacatc ggcgcggcgg
      301 tggccaccgc gcgactgctt ggcttggcgg cggaccagac gttggctgcg ctcggcaccg
      361 gtgctactca gtcggccggg atgaaggtcg tctacggctc gatgggcaag tcgctgcacg
      421 cgggcaaagc ggccgtggac gggctgctgt ccggtttcct tgcccgcgat ggcttcacgt
      481 cggccaccga gccgatcgaa gggcatcgcg gctttctaca tctcttctca cccgaccccg
      541 tcgccgagcg ggcggtcgag gggctcggag ccgtctggta tctgccgcgc gacgggttca
      601 agccgtatgc gtgcggctcc ttgacccacc cgcccgcgca ggcacttctc gagttgcggt
      661 ccgagcacgg cttgaccgcc gacgacgtcg ccgccgtcga cgcctacgtc cacgactacg
      721 tgaagaccac gaccgggctc gccgaaccgc gcaccggcct ggagggcaag ttcagcatct
      781 accacgtgct cgcggtcgcc ctcgccgacg gcgcggcatt actcgaccag ttcaccgatg
      841 agcgcgtcgc cgacctggaa ctggcccagc tgcggaagca ggttcacgtt cacaccgacc
      901 cggcgcagac caaggattcg gcgcgggtgg ttgtgacgct gcacgacgga cgcacgctgg
      961 aacgacacgt tgcgcacaat ctcggcaccc cggacaaccc gatgaccgat caacaactcg
     1021 aggacaagtt cgtcggattg gcttcgccgg tgctcggcca gggccgtacc gaagagctgg
     1081 ccacgacgtg ttggcggctg ctcgacctcg acgacatccg agccctgctg gcgctgacgg
     1141 tcccgggcta ggccgccgca ggggcaaacg cctcccgcag catcgcgcac tggctgttga
     1201 aaaacgactg cgacacatcg gctttcggca cgatcccgtc gaacccgtgg aacgcgccgg
     1261 cgacgacctc gacttcgcac ggcacaccgg cggccttgag ccgctcggcg taggccaggt
     1321 cctcgtcgtg aaacaggtcg aaggtgccga cacccagcca tgcggggggc aagccactga
     1381 gatcctcgcg tcgcgcgggc acggccacct gcgggtcggc cccgccgaga tatgcggacc
     1441 aaccgaactt attgcttgac tggttccaca gccgatgacc cggattctcc agatcccttc
     1501 ggcccacagt gcggtcgtcg agcatcggat acaccaaaag ctgtgccgca aggctgattt
     1561 cgccccgatc gcgggtgagc agggccagcg ccgctgccag cccgccgccc gcgctggcgc
     1621 cccctatggc cactcgtgcg ggatcaaccg agggcagccg aacaagccac tgcagcgccg
     1681 aatagcagtc ttccagcgat gcggggtatg ggttctcggg tgccaaccgg tactccaccg
     1741 acgcgacagt cacgccgagt tcgcgggcgt agcggcggca gagcacgtcg tcttgtgccg
     1801 ctcgaccgat gacgtaccca ccgccgtgga tccacaacag ccccggagcc tgacccgtca
     1861 cgccggtggg acggaaaagc cgaattccca ctcccgaggg cagcgtcagc acctcgatgt
     1921 ctttcggcgg tcggtgccac atcaaccgct cggccgcacg gatgaacggc accgtgaccg
     1981 gcgtgacgat ctgcttcggg gtgtagcggg cgacgcggcg caggtcgggg tggaaatggg
     2041 tgggctgcac ctttccagta ttgacgctca cttcagcagc gggtcccgcg ggaggccgag
     2101 aatccgttcg gcgatggtgt tgcgggtgat ctccgacgtg ccgcccgcga tggtcatggc
     2161 ccggttgccg agataactgc ggagcagctt cggcgcctgg ccgaccaccg ccgccgaacc
     2221 ggacaactcc atgcccaact cggtgatccg ctgtcccgcc tcggccacca gaagcttggt
     2281 gacgttgccc tccgggcccg gctccgcgcc cgcgatggcc cggctggccc ggcgcaggtt
     2341 cagcagccgc aacgtgtgcg tctccgcgat gcactcgccg gcccggcgcg cgtagttggc
     2401 cgccgacggc gaggcgtcga gcagcttgat gaggtgatcc gggctgaagc cgacggcgcc
     2461 accagaaccg ccgccgatcg agatgcgctc gttgcccagt gtcgcccggg ccacgagcca
     2521 gcctttgttc acgtcgccaa ccacgtcttc gtctggaacg aaaacgtcgt cgaagaagac
     2581 ctcgttgaag tgagagtcgc cggtgatgcc gcgcagcgga ttgactgtca caccaggggc
     2641 tttcatgtcg atcgccatca tcgtcacacc ggcatgcttg ggcgcgtctg gatcggtgcg
     2701 caccgtggcg agtccccact ggcagtactg ggccagactg gtccacacct tctgcccggt
     2761 gacccgccag ccaccgtcga ccttcttggc ggccgtgcgc accgcggcgg cgtcggagcc
     2821 ggcgcccggc tcggagaaca gctggcacca catcacctgg ccgcgcagca cgggttcgac
     2881 ccagcgctca cgctggtcgt cggtccctgc ctgcgcgatg gtgagcgcca cccagcccgt
     2941 gatgcccatg tcgggccgct cgatgccggc gaactcctcc tcgatgacca gctgctcgag
     3001 cacatcggcc gcgcggcccc acggtttcgg ccagtgcggc accaggtagc cggattcgac
     3061 gaggtagtcg cgttgcttgt cctgaggtag ggactgcaga tgcgcgacgg cctcgcgggc
     3121 ctggtcgcgg tgagcctccg cctcggcggg cagcgtgaac gacgcgccgt gcgcctggcc
     3181 gctgcgctgg ccctcgacga tatcgagcag cgggtcaccg ccctcctcca tcagcgcggc
     3241 cagtgtgcgg gcccgccgca ggtacaggtg ggcgtcatgc tcccacgtgt acgcgatgcc
     3301 gccgtgcagc tgaatgttgt actgcgcgtt gaagatctga gtacgaatgg cgtgcgcggc
     3361 ggcgacagcg gcggggaacc acgcactgtc aaggtcatcg gcgcgcgccg cgtcccaggt
     3421 ggccgcggtg gtctcctcgg cgttgaccaa catgttggcg gcatggtgct tgacggcctg
     3481 gaacgtgccg atggtccggc cgaactgttc gcgcaccttg gcgtattcga cggccatgtc
     3541 gagagccgcc cagctgacac cgaccgcctc ggccgacgcc aggatccgga acactgtcag
     3601 cgccttgcgt gcggcgccgc gaagcacccg gtccctttcg accgtcacgc cgcgaagcgc
     3661 gaccgtcccg acactgcggg tggtgtccag cgactccagc gccgtaatcg tcacgccgtc
     3721 ggccttcgcg tcgacaatga cgacgtcctc acccgcggcg atcaccagca cgtcggcgtc
     3781 cggcgcgccg agcacggcgg ggctttctcc ggtcacgacc aggtcggaac cgatcgtgac
     3841 gttgcccgcc agcgcgagcg caccgacggc gctgccgtcg gccagcgccg gaagtagttg
     3901 cgcgcgcacg gaatccggtg cgcaacggtc gatcaccacc gccgccgcga cgctgggcag
     3961 gaacgggccg ggggagagtt cgcgaccttg agcctccagc accaccgcca actcggcgag
     4021 gccgaagccc gaaccaccat ggtcctctgc aatcgcaagc ccgttccagc cgaggtcctt
     4081 gcccgcggac cagatctccg acggatgcgt ggcgccgttc tccagcgtcg cgcgggccgc
     4141 cgcgcggctg ttcagccggt tcagctgccc gatcgcggaa tcggccaggt cagtgtgttc
     4201 ttcggtgatg gcgagagctg acttactcac ggtggaagtc ctctgtaaag cgcggatgtt
     4261 gacggttgtc aatcgaagct aacgcgcccg agggcagctc agaagagacg tgatgcagtt
     4321 cacaaatgat cttgaaagtg tcacgggtag acatgttcgg aatgttgtcg tttgagagcc
     4381 ggactgcggg gtacagtcgc gcaatgacgg tacgaccgga cacccggtac cccggaccga
     4441 ccatatggac gcgtgcccgg tggttcctga aggccggacc ggccgactac atgctcgcga
     4501 tgagtgttgc gagcgcgtcc ctacccgtta tcgggaaaca ccttgagccg ctgggcgctg
     4561 cgacggcgat gggcgtctgg ggttaccgcc acatgccgga tttcctcggt gccaccgcgc
     4621 ggtcctggtt cgagccgggg aacgccgagg tacgcaagct ggaacgcgat tcaacccaca
     4681 acgtggtcga ggccgcactg cgtggcgttg tccgggctga ggaccttgac attgagtggc
     4741 ccgcgcccga gagcgccccg ccgctgtgga agttcaggca acatcggcgc agcgtgtacc
     4801 ggacgtcggt gcgatacggc aacctgccgt cgcagctgct cgacgtgtgg cgccggaagg
     4861 atctgcctgc cgagcccgcc cccgtgctga tcttcgtgcc gggtggtgca tgggtgcacg
     4921 gcagcaggct actgcagggc tacgcgctga tgtcgcatct ggccgagatg ggttgggtat
     4981 gcctgtccat cgattaccgt gtcgcgccgc accatcgctg gccggcgcac ctcaccgacg
     5041 tcaagaccgc gattgcatgg gcgcgcgcga acgtcgacaa attcggcggt gatcgcaatt
     5101 tcgttgcggt agcgggatgt tcggcaggcg gacacctgtc ggcactcgcg ggactgaccg
     5161 cgaacgatcc cgagatgcag tgcgaattgc ccgacggctc cgacacttcc gtcgatgcag
     5221 tggtgggcat ctatgggcgc tacgactggg aggaccggtc gacggcggag cgggcgcgct
     5281 tcgtcgactt cctggaacgg gtcgtggttg ggcgcaagat cgcaaagcac cccgacgtgt
     5341 tccgcaaggc gtctccgatc gcccgtgtgc acaaggacgc accgccgttc cttgtcatcc
     5401 acggcaccgg cgatagcgtc attcccgtcg agcaggctcg cagcttcgtc gcgaaactgc
     5461 gcgccgcgtc gcattcggtg gtcagctatg tggagctccc gggagcgggc cacgcgttcg
     5521 acatgatcga cggcgcccgc accggatcga tggcgaccgc aatcggcttg ttcctcaatc
     5581 agattcaccg gaaccgatcg ctcacgcggg ccaaacaggt gatctaggct tcctccgggt
     5641 aggagggagc actgtgaaga ggcttcgcgg gtgggacgct gtgctgctgt acagcgagac
     5701 gccgaacgtg cacatgcaca cgctcaagct ggcggtgatc caactcgacg atctcggtgg
     5761 ccgcacgttc gggatcgacg agttccgcaa ggtgatacat ggccggctcc acaagctcga
     5821 cccgttccgg taccagctga tagaggtgcc gggcaagatc caccacccga tgtggcggga
     5881 gaattgcgag gtcgacctcg actaccacgt gcgtccctac cgggtcgatg cgccgggcgg
     5941 tcggcgacaa ctggacgagg cggtcggcga gatcgccagc accatgtcgt tgatcgtcac
     6001 gcccaggtgt ttgccagtcg ctttgatgtc tgcgagcgct agcg
//
LOCUS       DASRJA010000049         4584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1086873,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000049 DASRJA010000000
VERSION     DASRJA010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086873"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..618)
                     /locus_tag="VFC01_02075"
     CDS             complement(1..618)
                     /locus_tag="VFC01_02075"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HZN78464.1"
                     /translation="MFQDLDATLAALLDAELAVRNVDISFAAPDDQFPPSGVTLPAVS
                     MFLYDIRENTQLRSNEWELRRDDDNRITSRRRPPVRVDCSYLITAWPSDVPEPAADEH
                     LLLGEVMKVLLRHRAVPAQYLRGDLEGQEPPVRARILAENQLQSLGELWQAMGGKPKA
                     ALHYGVTLSVDVSGWEVPGPKVFETVISLSQGVLPAEPAGTSSNP"
     gene            complement(819..3995)
                     /locus_tag="VFC01_02080"
     CDS             complement(819..3995)
                     /locus_tag="VFC01_02080"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HZN78465.1"
                     /translation="MNRFHRQQIAAANEPDGAVRDVVATAGERQSEPSELASFTGVGD
                     VLLAMQRACGNRDVQRLVPRPSGPVKRRLTLGPVSDCYEREADQVMEREGGGEVAGAP
                     SESEAQHARSDPPMPRLHAGAGASDPIDPLIQRVILGARGQGQGISATVRGPLERSLG
                     ADFGSVRVHTDSRADTLSRALQARAFTTGQDIFFRRGEFQPTSDSGRRTLAHELTHVV
                     QQTGPDAPAGCDDTTAAAPVQRLFTHDGTPITDGDLLQTILDLATGSVGSDEVADAFW
                     AAASVKAPNRDIGDWLALRDFSKKTVNKVIRLVEKSAIEPIQTKKRDRDDEPNHPPIT
                     AHGGTKTDAPKSMMLPPPQSEIAFKTGGGFGKNLARSKTQGTLSSFFKVTEPPPKRAA
                     DGTYRITSYQDLSEVPVLLVQKDALTSTYILADNEEAQEKYIRYDHSAKRYSHAVKER
                     VGSFNEWPPSVDKWKDGLLGIRAVKRTGKPVTGSLSSLGSVLSDEDLMRVYQVLGIWG
                     TWIVRRTLAAAKLGTISSIGAAIKKKMEGLLEGQQQDAGQFLSQLKGRQAAVFWGILK
                     DGPLNPLTALKTLTSAPSAQQFPAKSQTNYLGTVGELMDELRLKQRLRAKPSDRGTHL
                     LTHSTGSGEKGPDLILIKLRDGRYRINIHESKLTGWTPDNKNAGDQPTYGLSGLPAVA
                     RKGSKALKLYLTEAVTGHQLPDAKAIIDQIGTGEAVTTWKYATVGQVLLEGEGQHFTR
                     TKLGGTLSDPKKDALESWLNYARQLAKSASQTSNANDVGASSTTTSPVSSNLTPSSKP
                     LVSSLKPPVSSEPPVVDPDEMAVSLPEEFESSVTSSSNKVSSSQWKVSPQWKVKSNMP
                     PTLPITSSSKPMPGMLMRPVSDQPPVTSPTMPRSSFSQFLQSSSPPLAKKLKTVTGPA
                     PPPPVSDQPPVTSSTMPRSSFSQFLQSSSPPPASKLKTNKDPSPLTSSLLKLRPFPHL
                     AEGSSSSTPVVPSGPPTLSQGLQKMAAAEKVNRDGDKLIDELVNSLDPGNMHPGLRDY
                     LADLHRKQPTLDRSILRAFGGLWIDRQL"
     gene            complement(3992..>4584)
                     /locus_tag="VFC01_02085"
     CDS             complement(3992..>4584)
                     /locus_tag="VFC01_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN78466.1"
                     /translation="ATSLGLDLYKIDLSSVVSKYIGETEKNLARIFDEARTSNAILFF
                     DEADALFGKRTQVRDAHDRYANVEVSYLLQKMEEYEGVVILATNLRKNMDDAFVRRLH
                     FTVEFPMPDEADRRRIWEQIWPELTPRDPGLDLDRLARDVPLTGGSIRNIAVAGAFLA
                     AADGSVVTMQHLHHATEREYQKMGKVITGDGREGMP"
BASE COUNT          758 a         1520 c         1418 g          888 t
ORIGIN      
        1 tcatgggttg gacgacgtgc ccgcgggctc ggccggcagc acgccctggc tgagactgat
       61 gactgtttcg aagaccttgg ggcctgggac ctcccagccc gagacgtcga cgctgagggt
      121 gaccccgtag tgcaaggccg ccttcggctt gccgcccatc gcctgccaca gctcgcccag
      181 gctctgcagc tggttctcag cgaggatgcg cgcccgcacc ggcggctcct gcccttcgag
      241 gtcgccgcgc aggtactgcg ccggaacggc ccggtgccgc agcagcacct tcatgacctc
      301 gcccagcaac aggtgctcgt cggcagccgg ctcgggcacg tcgctgggcc acgcggtgat
      361 gaggtacgag cagtcgaccc gcaccggcgg ccgccgccgc gaggtgatgc ggttgtcgtc
      421 gtcgcgccgc agctcccatt cgttgctgcg tagctgggtg ttctcgcgga tgtcgtagag
      481 gaacatcgac accgccggca gggtcacccc cgacggcggg aactggtcgt ccggcgccgc
      541 gaagctgata tcgacgttcc gtacggccag ctcggcgtcg agcagtgcgg cgagggtggc
      601 gtcaagatcc tggaacatgc tggcccgaga caccgtccat gagcggtcgc tcgctcccgc
      661 cctccaccgc tctccgcctc gctcgcaccc cgcctctttc cccctagacc acctcgccga
      721 agcgatatta actgactaat aaattggtaa acaatgacag atcgatgaca ggcacgacgt
      781 cgattcagca ggccctaccg ctccacaggc cgcatcggtc agagttgcct atcgatccac
      841 aggccgccga aggcacggag aatgctccta tcaagtgtgg gctgtttgcg gtgtaggtcc
      901 gcgaggtaat cccgcagccc cgggtgcatg ttgccggggt ccagcgagtt gacgagttcg
      961 tcgatcaact tgtccccgtc gcggttcacc ttctcagccg ccgccatctt ctggaggcct
     1021 tgcgacagcg ttggcggccc acttggcacg accggcgtcg agcttgacga gccctccgcc
     1081 aggtggggga acggccggag cttcagcaac gacgaggtga gcggcgacgg atccttgttc
     1141 gtcttgagct tgctcgccgg cggcggcgac gacgactgca agaactgcga gaaactcgac
     1201 ctcggcatcg tcgacgacgt caccggcggc tgatccgaca ccggcggcgg cggcgctggg
     1261 cccgtgaccg tcttgagctt cttcgccagc ggcggcgacg acgactgcaa gaactgcgag
     1321 aaactcgacc tcggcatcgt cggcgacgtc accggcggct gatccgacac cggccgcatc
     1381 aacatgcccg gcatcggctt cgaagacgaa gtgatcggca gggtcggcgg catgttcgac
     1441 ttgaccttcc actgcggcga gaccttccac tgcgacgatg acaccttgtt cgacgacgac
     1501 gtcacgctcg actcgaactc ctcgggcaac gacaccgcca tctcatctgg atccaccacc
     1561 ggtggctccg acgacaccgg cggcttcaac gacgaaacca gcggcttgga cgacggcgtc
     1621 aagttcgacg acaccggcga cgtcgtcgtg gacgatgcgc ccacgtcgtt ggcattcgac
     1681 gtctgcgacg cagacttggc gagctgtcgc gcatagttca gccagctctc cagcgcgtcc
     1741 ttcttcggat cggacagcgt cccgccaagc ttcgtgcgag tgaagtgctg accctccccc
     1801 tcgaggagga cctgtccaac cgtcgcgtac ttccaagttg taacggcctc tcctgttccg
     1861 atctggtcga tgatcgcctt cgcatcgggt agctgatgtc ccgtcacagc ctcagtcaga
     1921 tacaacttga gtgccttcga gcccttgcgg gcaactgccg gcaggccaga caatccatac
     1981 gtcggctggt ctcctgcatt cttgttatcc ggtgtccagc cggtgagctt ggattcatga
     2041 atattgattc tgtaacggcc atcccggagc ttgatgagga tgagatcagg gcccttctcg
     2101 cccgaccccg tcgaatgagt caatagatgc gttccacggt cggacggctt ggcccgaagc
     2161 ctctgcttca gtctgagctc atccatcaac tcgccgacgg tgcccagata gttggtttga
     2221 gacttagcgg gaaattgctg agctgatggc gcactcgtca gggtcttgag ggccgtcagg
     2281 ggattcaaag gaccgtcctt gaggatcccc cagaagacgg ctgcttgcct ccccttcaac
     2341 tgggacagga attggccggc gtcctgttgc tggccctcca gcagaccttc catcttcttc
     2401 ttgatcgccg caccaatgct ggagatggtg ccgagttttg cggcggcgag ggtccgccga
     2461 acgatccacg taccccagat ccccaagacc tggtagaccc gcatgagatc ttcatcactc
     2521 aggacggaac cgagggacga cagtgaaccc gtgaccggct tgcccgtcct cttgactgcc
     2581 ctgatcccga gcaggccatc cttccacttg tcaacgctag gcggccactc gttgaagctt
     2641 cccacacgct ccttgaccgc atgcgagtac ctcttagcgg aatggtcata gcggatatac
     2701 ttctcctgtg cctcttcatt atcggccagt atatatgtac tggtcaaggc gtccttttgc
     2761 accaagagga ccggcacttc ggacaagtcc tggtacgacg ttatgcgata cgtaccgtcc
     2821 gctgcccttt tcgggggagg ctccgttact ttgaaaaacg aactgagcgt cccttgggtc
     2881 ttcgatcggg cgagattctt gccgaagcca cctccagtct tgaacgcgat ctccgactgc
     2941 ggcggaggca gcatcatgct cttcggcgcg tccgtcttgg ttcctccatg agcggtgatg
     3001 ggcgggtggt tcggctcgtc gtctcggtca cgcttcttgg tttgaatggg ctctatcgca
     3061 ctcttctcga ccagacggat caccttgttc acggtcttct tactgaagtc acgcagggct
     3121 agccagtcgc cgatgtccct attcggcgcc ttcactgacg cagcagccca aaaagcatca
     3181 gcaacttcgt cgcttccaac cgatcctgtt gcaaggtcaa gaatcgtctg tagaagatct
     3241 ccgtccgtta tcggcgtgcc gtcgtgggtg aacaaccgtt ggaccggcgc ggccgctgtc
     3301 gtgtcgtcac agccggcggg cgcgtcgggg cccgtctgtt gcacgacgtg ggtgagctcg
     3361 tgggcgaggg ttcggcggcc gctgtcgcta gtgggctgga actcgccgcg gcggaagaag
     3421 atgtcctggc cggtggtgaa ggcgcgtgcc tgcaacgccc tgctgagagt gtcggcccgg
     3481 gagtcggtgt ggacccgcac cgagccgaag tccgctccca acgaccgctc cagcggcccc
     3541 cgcactgtgg ccgagattcc ctggccctgg ccgcgggcac ccaagatgac ccgctggatg
     3601 agcgggtcga tcgggtcgct cgctcccgct ccggcgtgca ggcgcggcat cggcgggtcg
     3661 gaccgggcat gctgcgcctc cgactccgac ggagcaccgg cgacctcgcc gccgccctcc
     3721 cgctccatca cctggtccgc ttcgcgctcg tagcaatcgc tgaccgggcc gagcgtcagg
     3781 cgccgcttca ccggtccaga cggtcgcggt accagccgct gcacgtcacg gttcccgcag
     3841 gctcgctgca tcgccagaag gacgtcgccg acgccggtga atgacgccaa ctcggacggc
     3901 tcggactggc gctcccctgc cgtcgccacg acgtcccgga cggcgccatc tggctcgttc
     3961 gctgcagcga tctgctggcg gtgaaaccgg ttcatggcat cccctctcgc ccgtcaccgg
     4021 tgatgacctt gcccatcttc tggtactcac gctcggtggc gtgatggagg tgttgcatgg
     4081 tgacaacgct gccgtcggcc gcggccagga atgctcccgc caccgcgatg ttgcggatcg
     4141 agccgcccgt cagaggcacg tcccgggcca gccggtccag gtcgagccca gggtcgcgcg
     4201 gggtgagctc cggccagatc tgctcccaga tgcggcggcg atcggcttcg tccggcatcg
     4261 ggaactcgac ggtgaagtgc agccggcgga cgaaggcgtc gtccatgttc ttgcgcaggt
     4321 tggtcgccag aatcaccacg ccctcgtact cctccatctt ctgcaggagg tacgacacct
     4381 cgacgttcgc gtagcggtcg tgcgcgtctc ggacctgggt gcgtttgccg aacagggcat
     4441 cggcctcgtc gaagaacagg atcgcgttgc tggtccgggc ctcgtcgaag atccgcgcca
     4501 ggttcttctc ggtctcgccg atgtatttgc tgaccacgct ggacaggtcg atcttgtaga
     4561 ggtcgagccc cagcgaagtg gcaa
//
LOCUS       DASRJA010000050         6976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1405272,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000050 DASRJA010000000
VERSION     DASRJA010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405272"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFC01_02090"
     CDS             <1..579
                     /locus_tag="VFC01_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN78467.1"
                     /translation="GAICVGGVDVREIDPAVLYRHVGLVLQDVQLLGISVADNIRLGR
                     PDASDDDIVSAAKAAYIHDRITALPNGYDSVVGDDAHFSGGEAQRVSIARAVLADTPI
                     LVLDEATAFADPDSEAQIQAALSHLIVGRTVLVIAHRLGSIVGTDNIVVLNDGRVAEQ
                     GQHDELLARGGQYARMWASYTAAAAKEWEVAQ"
     gene            576..965
                     /locus_tag="VFC01_02095"
     CDS             576..965
                     /locus_tag="VFC01_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78468.1"
                     /translation="MIRGFLRILGPQRGCMYAYLAWVTLYGLLHGVAMILLVPISVAL
                     FDERYADAGRWLGVLAVVVVVAALAHYVQATLAIRMALTTMRLLHHRLGDHMVTLPLG
                     WFNRETVGHVSQIAVKGTVFVGTSGAN"
     gene            1365..1676
                     /locus_tag="VFC01_02100"
     CDS             1365..1676
                     /locus_tag="VFC01_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78469.1"
                     /translation="MATSTRSRWSRSSVWPPRFASPLAALAEIGSAMRMAATELRRIT
                     TVLDTPALPEPSTPQPVSVPGRVDLDHVAFSYPGRQVLSDISFTAEPGTMTALVAVGF
                     R"
     gene            complement(1685..2137)
                     /locus_tag="VFC01_02105"
     CDS             complement(1685..2137)
                     /locus_tag="VFC01_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78470.1"
                     /translation="MKAMMTAVLAGGVLVAAGVASVGHDEPTPTSAPRVQGKPTVTAL
                     VTPNRLYAATGAPPVVVFGTNGSLGIGGAHTAAGPGATPIDRPTPAAVDHDRGLMSTS
                     MFFAPTTFARPASTPSDSRSAVSVSVAPVRRPHAPLRCRHRRTARATA"
     gene            complement(2163..2447)
                     /locus_tag="VFC01_02110"
     CDS             complement(2163..2447)
                     /locus_tag="VFC01_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HZN78471.1"
                     /translation="MKRFIIEREIPGASDLTEAELAEISKKSKTAMASLGVPYRWVKS
                     YVAGDKIYCVHEAESEDVVREHSRRGGFPVNNVAVVANEFGPQTAEHVTS"
     gene            2643..5231
                     /locus_tag="VFC01_02115"
     CDS             2643..5231
                     /locus_tag="VFC01_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN78472.1"
                     /translation="MILAEREGELDQLVARANEARSGRGGVVVVCGESGAGKTSFVET
                     FLERWPQDERVLWGACDPLDTPRPLGPIQDLADQLAPDTRDVVRVSNYPHEIHGAVFE
                     ELAAQPSVLFLDDMHWADQGTVDLLRYVLRRIQRTRSLAVLTVRDDEIAATHPMRSLL
                     GDVARSNSATSLALSPLSIEAITQLAQDRLVDPVRLHRITGGNAFFVVEMLDQTGDEL
                     PTTVRDAILARTIGLDGAAWDLLYLMACAPESIPDYLLAHLGVTVPALRTLDEVKLIR
                     RTDRGVTFRHDLCRLAVASVIPPGAEPELHRRMIDAYEAASRTDPAVLTHHALGAGDC
                     ERIQRAATDAGKAATRSGAHTQAAEFYRIALEEGGELPAETEAELLELLAAEYYLTDR
                     LDDAIKACQRAMDLRQLMGASANVSADHHSLSVYEWYNANRRAADHHVAQAVAVLDGQ
                     IEDESPTGLAELGHAFAMQAYLAMQTTDLEHAAALAARANEIADIAGDPSLVVRVRLI
                     EGYLAVLEGKDHGRESILSIVRSNPENFDEIYSGGYSNLTYMDVEQRRFSLAENLLDE
                     SIAITIERDLPICYAWQMGARGRLGMLTGNWDDAVADANTVLDAPSAPLARTWPLLIR
                     ALVALRRDGTRRDDIDEAWRLACSYGEPLRVLPAAAAIIESSWLSGTLDDRLDECRTL
                     LDNTPIIGLEWARGEVAVWLHRLDASLQADGLAVPYALLLDGKFEAAAEEFHRLSMPY
                     EAALALIDSSDADLARRGLDVLDRLGAAAVAAKVRRDLRSRGVPVVPARRRSTTLANP
                     AGLTTRQVEVLRLIEEGLTNAELADRLYLSAKTVDHHVSAILTKLGVPGRRDAIKRGR
                     ELGILA"
     gene            complement(5228..6094)
                     /locus_tag="VFC01_02120"
     CDS             complement(5228..6094)
                     /locus_tag="VFC01_02120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN78473.1"
                     /translation="MDLSGVGIWSNQLRYGDSAESAEAAAELEELGFTALWIPDIGGP
                     VLDSVDSLLSSTKRTVIATGILNLWMHEPSDVAARYASLTNTHGERFLLGIGVSHAPL
                     IDTREPGRYRKPLAAMRSFLDALDAADHPVPVENRVLAALGPKMLELSATRARGAHPY
                     LVTPDHTRQAREVLGDGPLLLPEQAVLLADNRDEARAVGTDWLRTYTTLPNYANNLLR
                     SGFSQDDLDSVSDRLFDAIIAWGDEDAIVRRVDEHKQAGADHVCIQALTTEPLGFPRE
                     HWRRLAAALTKG"
     gene            6143..6529
                     /locus_tag="VFC01_02125"
     CDS             6143..6529
                     /locus_tag="VFC01_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN78474.1"
                     /translation="MAFDPGAVGRAFSGHRFDEALPHLAKDVRWTIVGYMVLEGADAV
                     RQTCRETLESLEGTTTTYDRCVVAAGSDTVAVDTFARYTRPDGMTAVASCDIYEFSGE
                     QISAITSYAVEVDPQDAGSQPPPRSV"
     gene            6546..>6976
                     /locus_tag="VFC01_02130"
     CDS             6546..>6976
                     /locus_tag="VFC01_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN78475.1"
                     /translation="MTVEDFPIGRDATLHELEEDPHSLLARLRAAEPVSWLAALDGWL
                     VTRYDLAVRVMRDPKTFTVDDPRFSTARVVGPSMLSLDGAYHARHREPFAHPFRPALV
                     RDRFSGFVAAEADRLISAIQPAGSADLRREFAGPLAVAVVA"
BASE COUNT         1122 a         2253 c         2348 g         1253 t
ORIGIN      
        1 ggcgcgatct gcgtcggcgg agtcgacgtg cgcgagatcg acccggcggt gctgtaccga
       61 cacgtcgggt tggtgctcca ggatgtccaa ctgctcggca tcagcgtcgc cgacaacatc
      121 cggttgggcc ggcccgacgc cagcgacgac gacatcgtca gcgcggccaa ggccgcctac
      181 attcacgacc gaatcaccgc cttgcccaac gggtatgact cagtagtcgg tgacgacgcg
      241 cacttctccg gcggcgaggc acaacgtgtc agcatcgcac gcgccgtgct ggcggacacg
      301 ccgatcctgg tcctcgatga ggcgacggcg ttcgccgacc cggactcgga ggcgcagata
      361 caggccgcgc tgtcgcatct gatcgtcggc cgcacggtac ttgtcatcgc ccaccgcctc
      421 ggctcgatcg tcggcaccga caacatcgtc gtactcaacg acggacgcgt tgccgagcag
      481 ggccagcacg acgaactgct ggcgcggggc ggtcaatacg cgcggatgtg ggccagctac
      541 accgccgctg cggccaagga gtgggaggtg gcccagtgat ccgggggttt ctgcgtattc
      601 tcggcccgca gcgcggctgc atgtacgcct acctggcgtg ggtcacgctc tacggcctgc
      661 tgcacggcgt cgcgatgatc ctgctggtgc cgatctcggt ggcgctgttc gacgagcggt
      721 atgccgacgc gggccggtgg ctgggcgtgc ttgccgtcgt cgtggtcgtc gccgcgctgg
      781 cccactacgt ccaggcaacg ctggcgattc ggatggcgct gaccacgatg cggctgctgc
      841 accaccggct gggtgaccac atggtgacct tgccgctggg ctggttcaac cgcgagaccg
      901 tcggtcatgt gtcccagatc gcggtcaagg gaaccgtttt cgtcggtacc agcggcgcga
      961 actgatcacc ccggtggtgc tcaattccac cagcgcggcg acggtggtgg tcgggctgtt
     1021 cgtctacgac tggcggatcg gtctggtcgc gttggccggc ggcgtgctgc tggcggcgtg
     1081 cgggaggttc gtgtcgggtt tgatcgcctt tgcagagagt caaacccatg acgccaccgt
     1141 ccaagtcaac actcgagtaa tcgagttcgc gaagtgtcag ccggtactgc gggcgttcgg
     1201 ccgtgccggc agcggctacg aacccttgtc ggatgctctt gaaactcagc aacgggtcgg
     1261 ccgcagcgcg ctgtggcaat cggtggccgg tttgatgctc aacggtgttg cggtgcaggc
     1321 agttttcttg gtgctcatcg ctgtcggcgc gtggatcgcg gtcggtggcg acatcaaccc
     1381 gatcacgttg gtcgcgatcc tcggtttggc cgccccggtt cgcgtcaccg ttggctgccc
     1441 tcgccgagat tgggtccgcg atgcggatgg cggcgaccga actgcgccgt atcacaacgg
     1501 tcctcgacac ccccgcgctt ccggaaccgt cgacaccgca acccgtgtcg gtgccaggtc
     1561 gggtcgacct cgaccacgtg gcgttcagct atccgggtcg tcaggtgttg tcggacatca
     1621 gctttaccgc cgaaccgggg acgatgaccg cgctcgtcgc cgtcgggttc cggtaagacg
     1681 accatcacgc ggttgctcgc gcggttctac gatgtcgaca gcggagtggt gcgtgtgggc
     1741 ggcgaacggg cgccacggag acggagacgg ccgatcgcga atccgacggc gttgatgccg
     1801 ggcgcgcaaa ggtggtgggc gcgaagaaca tcgacgtcga catcaggccg cggtcatggt
     1861 ctaccgcggc aggtgtgggc ctatcgatgg gggtcgcgcc cggtccggcg gcggtgtgcg
     1921 cgccgccgat cccgaggctt ccgttggtgc cgaagaccac gactggcggg gctcccgttg
     1981 ccgcgtacaa gcggttcggt gtcaccagcg ccgtgacggt cggcttgccc tggacgcgcg
     2041 gtgcgctcgt cggtgtcggc tcgtcgtgtc cgacggaggc cacgccagcg gcgacgagca
     2101 cgccgccggc cagcaccgcc gtcatcatgg ccttcatgtc gctccccttc tcctgagttc
     2161 ggtcagctgg tgacgtgttc ggccgtctga ggaccgaatt cgttggcgac gacggccacg
     2221 ttgttgaccg ggaaaccgcc gcggcgcgag tgctcgcgaa ccacgtcttc gctctcggcc
     2281 tcgtggacgc agtagatctt gtcgccggcc acgtagctct tgacccaccg gtacggaacg
     2341 cccagtgagg ccatcgcggt cttggacttc ttcgagatct cggccagctc ggcctcggtc
     2401 aagtcggaag ccccggggat ctcgcgctcg atgatgaatc gcttcatggt gtcaactcct
     2461 gttgtgtgtt tttgtccgcc gggttcggcg tgcgtaactc acgctcggcg atcgaggcgc
     2521 cccagacatc gggagacgtt ccccatattg ctgaccgggt cggcatcccc tatgtgcacc
     2581 acagtcctgc caggtaggcg tttcaccagc tcagcacccc agatcgggtt agtattcgct
     2641 gcgtgatact cgccgagcgt gagggcgaac tcgatcaact cgtcgcccga gccaatgagg
     2701 cacgctcggg tcgcggaggc gtggttgtcg tctgcgggga atcgggtgcc ggcaagacgt
     2761 cgttcgtcga gaccttcctc gagcggtggc cgcaggacga gcgggtgcta tggggtgcct
     2821 gcgatccgct ggatactccg cggccgctgg gaccgatcca ggatctcgca gaccagctcg
     2881 cgccggacac acgcgacgtg gtgcgggtga gcaactaccc gcacgaaatc cacggcgctg
     2941 tcttcgagga gctggcagcc caaccgtcgg tcctgttcct cgacgacatg cattgggcgg
     3001 atcaaggaac ggtcgacctg ctccggtacg ttttgcggcg gatccagcgc acccgctccc
     3061 ttgcggtact gacggtgcgc gacgacgaga tcgcggcgac gcacccgatg cgctcgctac
     3121 tcggcgacgt cgctcgctcc aattcagcga catcgcttgc cttgtcgccg ttgagcattg
     3181 aggcgattac ccagctcgcc caggaccggc tggttgatcc cgttcggctg catcgcatta
     3241 cgggtggcaa cgcgttcttc gtcgtggaga tgcttgacca aaccggcgat gaactgccga
     3301 cgaccgtccg cgatgcgatc ctggcgcgga cgatcggtct cgacggcgcg gcatgggacc
     3361 tgctgtatct gatggcatgt gcaccggagt cgattccgga ttacctgctg gcccacctcg
     3421 gtgtgacagt gccggcgctg cgcacgctgg atgaggtcaa gctcatccgc cgcaccgata
     3481 gaggcgtcac cttccgccac gacttgtgtc gactcgccgt ggccagcgtc atcccgccgg
     3541 gcgccgaacc cgagctgcac cggcgaatga ttgacgccta cgaagcggcg tcgcgaaccg
     3601 atcccgccgt tctcacacac catgccctcg gtgccgggga ctgcgagcgc atccagcgcg
     3661 cggcgacaga cgcgggaaag gcggcgacgc ggtccggcgc acacacgcag gcggcggagt
     3721 tctatcggat cgcgctcgag gagggtggtg agttacccgc ggagaccgag gccgagctgt
     3781 tggagctgct ggccgccgag tactacctga ccgatcgtct cgatgacgcg atcaaggcgt
     3841 gccagcgcgc gatggatctc cgtcaactga tgggcgcctc cgccaacgta agcgccgatc
     3901 accactcgct gtcggtgtat gagtggtaca acgcgaaccg aagggccgcc gaccatcacg
     3961 tcgcccaagc cgttgcggtg ctcgacggac agatcgaaga cgaaagtccc acgggactgg
     4021 ccgaattggg ccacgcgttc gcgatgcagg catacctcgc catgcagaca accgatctgg
     4081 agcatgcagc ggcgctcgcg gcgcgcgcga acgagattgc cgacatcgcg ggcgacccat
     4141 cacttgtcgt gcgtgtcaga ctcattgagg gttaccttgc ggtgctggaa ggaaaagacc
     4201 acggccgcga atcgatcttg tcgatcgtgc gatccaatcc cgagaacttc gacgaaatct
     4261 attcgggcgg atacagcaat ctgacctaca tggatgtcga gcagcgaaga ttcagtctgg
     4321 ctgagaatct gcttgacgag agcatcgcga tcacgatcga gcgtgacctg ccgatctgct
     4381 atgcgtggca gatgggcgcg cgcggcaggc tcgggatgct cactggtaac tgggacgacg
     4441 cggtcgccga cgccaacacc gtgctggatg ctcccagtgc gccgctagcc cggacctggc
     4501 cgctgctcat ccgggctctc gtcgcgcttc gtcgcgacgg gacgcgccgc gacgacatcg
     4561 acgaggcgtg gcggctcgcg tgcagctacg gtgagccgct gcgggtgcta ccggcggcgg
     4621 cggcgatcat cgaatcgtcc tggctgtcgg gcacattgga cgaccgactc gacgagtgcc
     4681 gcacactgct cgacaacaca cctattatcg gcctggaatg ggcgcgcggt gaggtggcgg
     4741 tatggctgca ccgtctcgat gcctcgctcc aggccgacgg gctcgcagtt ccctacgcgc
     4801 tgctcctcga tggcaagttc gaagcggcgg cggaggaatt ccaccggctc tcaatgcctt
     4861 acgaggcggc gctcgctcta attgactcca gcgacgccga cctggcccgc cgtggcctcg
     4921 atgtactgga ccggctgggt gcggccgccg tcgccgcaaa ggtccgccgt gatctgcgct
     4981 cgcgcggagt ccccgttgtg cccgctcggc ggcgctcaac cactctcgcc aatccagcgg
     5041 gtttgacgac gcggcaagtt gaggtgctac gcctcatcga ggaggggctc accaacgccg
     5101 agctggccga ccggctttac ctgtctgcca agacagttga ccaccacgtc tcggcgatac
     5161 tcaccaagct tggtgtgccc ggacggcgcg acgccattaa gcgaggccgc gaattgggca
     5221 tcctcgctta acctttggtc aaggccgcgg cgagtcggcg ccaatgttca cgcgggaacc
     5281 ccagcggctc ggtggtgagc gcctggatgc agacgtggtc cgcacctgcc tgcttgtgct
     5341 catcgacccg ccgcacaatg gcgtcctcgt cgccccatgc gatgatcgcg tcgaacagtc
     5401 ggtcactgac ggagtcaagg tcgtcctggc tgaagccgga gcggagcagg ttgttggcgt
     5461 agttcggcag cgtcgtatag gtacgcagcc aatcggttcc gacggcgcga gcctcgtcgc
     5521 gattgtcggc gaggagaacc gcctgctcgg gcagcagaag ggggccgtcg ccgagcacct
     5581 ctctggcctg gcgggtgtga tcgggcgtga cgaggtaggg atgagcgccg cgggcccgcg
     5641 tcgccgacaa ctcgagcatc ttggggccga gcgcggcgag gactcggttt tcgacgggca
     5701 ccggatggtc ggcggcatcc aaagcgtcga ggaacgaccg cattgcggcc agcggcttgc
     5761 ggtagcggcc gggctctcgt gtatcgatca gcggggcgtg gctcacgccg atgccgagca
     5821 ggaagcgctc gccgtgcgtg ttggtcaacg acgcgtacct ggcggccacg tcagatggct
     5881 cgtgcatcca gagattcagg attcctgttg cgatcactgt gcgcttggtc gatgacaaca
     5941 gtgaatcgac agaatccaag accggaccgc cgatgtcggg aatccacagc gcggtgaatc
     6001 cgagctcctc cagctcggcg gcggcctccg cggactcggc cgagtcgccg taccgcagtt
     6061 gattactcca tatgccgacg cccgacagat ccatgcggtc atcctttcca ctcacctgac
     6121 aggctcacgc taccgtcggc agatggcatt cgatcccggc gccgtcggcc gcgcgttctc
     6181 cgggcaccgg ttcgatgagg cactgccgca cctcgccaag gatgtgcgtt ggacgatcgt
     6241 cggatacatg gtgctcgaag gcgccgacgc ggtacggcag acctgccgcg agacgctgga
     6301 aagccttgag ggcacgacga ccacctacga ccggtgtgtg gtcgcggctg gctcggacac
     6361 cgtcgcggtc gacacgttcg cccgctatac acgtcccgac ggaatgaccg ccgtggccag
     6421 ttgcgacatc tacgaattca gcggggaaca gatctcggcg atcacgtcat acgcggtcga
     6481 ggtcgaccca caagatgcag gctcacagcc cccacctcgg tctgtctagt cggccatctt
     6541 tcttgatgac tgtcgaggat ttcccgatcg ggcgcgacgc gaccctgcac gagttggagg
     6601 aagatccaca ctcactattg gcacggctgc gggcggccga gcccgtgtcg tggctcgccg
     6661 cattggatgg gtggctggtc acccgctatg acctcgcggt gcgagtgatg cgtgatccca
     6721 agaccttcac cgtcgacgat cctcggttct ccactgctcg ggtggtcggg ccgagcatgc
     6781 tctcgctcga cggggcgtac cacgcacggc accgcgagcc attcgcgcac ccgttccgcc
     6841 cggcattggt gcgggaccgg ttcagcgggt tcgtcgcggc ggaggccgat cggttgatct
     6901 cagcgatcca accggccggc agcgccgatc tgcggcgtga gttcgccggg ccgctcgcgg
     6961 tcgcggtggt cgcaaa
//
LOCUS       DASRJA010000051         9868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3900454,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000051 DASRJA010000000
VERSION     DASRJA010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9868
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3900454"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFC01_02135"
     CDS             complement(<1..249)
                     /locus_tag="VFC01_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HZN78476.1"
                     /translation="MSGMPPVEKYILLPVLMLHDKIYKGTNGRIGHRILPGTPPNLLL
                     HTVGAKTGIHRTNSLTYAKDGDTYLVVASKGGDPKAPGW"
     gene            complement(255..692)
                     /locus_tag="VFC01_02140"
     CDS             complement(255..692)
                     /locus_tag="VFC01_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HZN78477.1"
                     /translation="MSCVFCAIVADEAPAIRIYEDDDYLAILDIRPFTRGHTLVIPKR
                     HTVDLTDTPPDTVAALVRIGHRIARAARQSGLHADGNNVVINDGQAAFQSVPHIHLHV
                     LPRQNGDKLSFAKGMLLRRDPDRDESGRLLREALAELDSATQD"
     gene            complement(709..1182)
                     /locus_tag="VFC01_02145"
     CDS             complement(709..1182)
                     /locus_tag="VFC01_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN78478.1"
                     /translation="MSVKPIPEGYTSLTPFLCIDGAAAAIDFYATVFGAKVVDRMDGA
                     GGTVAHAELDFGNGRLQLGDPAEAYHIAAPDPTAPATHSVAYYCEDVDAVVAKAEQAG
                     ATIREPAQTFVTGDRFASILDPFGQRWTVMTRVEDVSGEERDRRMAEWAKENVGG"
     gene            1181..1537
                     /locus_tag="VFC01_02150"
     CDS             1181..1537
                     /locus_tag="VFC01_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78479.1"
                     /translation="MSASFGDRAVAVLEKRDMATLARVHDDLNLDDEFADESESEDAA
                     LEDENAHTLSIRRATGEEVLSVFSPHEEWSVYLQPGGGITNAFVGTPGEPIVWVNAND
                     RRVERDEDGTYVIRID"
     gene            1762..3165
                     /locus_tag="VFC01_02155"
     CDS             1762..3165
                     /locus_tag="VFC01_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN78480.1"
                     /translation="MDDEEDPVVSEQAQPPTAEPEAPPSVVRKAVAASAIGNATEWFD
                     YGIYAYGVTYISAAIFPGDTATATLLALMTFAVSFLVRPLGGFVWGPLGDRLGRKRVL
                     AITILMMAGATLCVGLVPSYAAIGLWAPILLVLLRMVQGFSTGGEYGGAATFMAEYAP
                     CRRRGFLGSFLEFGTLAGFSVGALLMLGFSTVLGDDQMHAWGWRLPFLVAAPLGLVGV
                     YLRTRLEDTPIFRELEAKGQQEHETTTEFKDLLAGYWRPILRLGGLVVALNVVNYTLL
                     SYMPTYLEGEIGLSADQSLVVPIIGMLTMMVFLPFAGGLSDRIGRKPLWWFSLAGLFV
                     AGVPMFLLMGTGLAGAVIGFAVLGLLYVPQLATISATFPAMFPTHVRYAGFAIAYNVS
                     TSLFGGTAPAVNEWLIGRTGDGLVPAYYMMAACVIGAIALITVPETTRCPIGGRAVPG
                     TKEAPPPVAYNPVPAPA"
     gene            complement(3194..4447)
                     /locus_tag="VFC01_02160"
     CDS             complement(3194..4447)
                     /locus_tag="VFC01_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZN78481.1"
                     /translation="MAELSQILKQATGVLAARGEGVQLYDEDDRRYLDFTAGIGVTST
                     GHCHPRVVEAAQRQVATLIHAQYTTVMHRPLLTLVERLGEVLPPGLDRMFFANSGSEA
                     IEAALRLSRQATGRPNVIVFHGGFHGRTIGAASMTTSGTKFRSGFSPLMGGVHVSPFP
                     DPGHFGWPVEQATDFALAQLDYVLQTLSAPADTAAFFVEPVLGEGGYVPANERFLAGL
                     RERADAYGILLVLDEVQTGFGRTGRFWGGDHFNARPDIVVTAKGLASGFPLSGIAASS
                     ALMEKAWPGSQGGTYGANAVACAAAIATLDVIQDERLVENSAERGAQLKQALQTVADK
                     HDQIADVRGLGLMIGNEFRDADGKPDPTTAAAVQQEAAKRGLLLLTCGAWSQVVRFIP
                     ALVVNADEVDEAAGIWADAMGAVVT"
     gene            4530..5993
                     /locus_tag="VFC01_02165"
     CDS             4530..5993
                     /locus_tag="VFC01_02165"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011596920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZN78482.1"
                     /translation="MSEHEAQVVKAVEKRLFIDGKWVDAADGGTFDVIDPATGATLCA
                     VADATPADGRAALDAAVAAQPGFAATAPRERADMLTGAFDLLHERIDDLALLMTLEMG
                     KPLAEARGEIAYAAEFFRHFAGEAVRIDGGYQTAPAGGARFLVTRQPVGPCLLITPWN
                     FPMAMGTRKLGPAIAAGCTSVVKPAHQTPLSMLALMDILTEAGVPAGAVNCITAMDAG
                     AVMEPLIRSGLARKLSFTGSTRTGRLLLEQCAEKILRTSLELGGNAPLIVFEDADLDE
                     AVAGAIAAKMRNMGEACTAANRVYVHSSVIDEFGRRLAERMAAMRVGRGTEDGVQVGP
                     LIDEAALQKVESLVADAVGRGATVLTGGSASPGDGYFYPPTVLTGVPRDADMADQEIF
                     GPVAPLTPFDTEAEVLAAANDTEYGLVSYVFTNDLRRALRVAEALETGMVGLNQGVVS
                     NPAAPFGGVKQSGLGREGGAVGIDEFLETKYVGIAMN"
     gene            complement(5990..6655)
                     /locus_tag="VFC01_02170"
     CDS             complement(5990..6655)
                     /locus_tag="VFC01_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN78483.1"
                     /translation="MTSYIEPLMQQSTPSIIAEKLRQAIGHGELKPGAQLSEAELARK
                     LGVSRGPLREGMQRLTQEGLLVSIRHRGLFVIEMTTADVRDMYLAREAIERAAAHKII
                     EGDYVAAGDALLEIVEQMAAATTPAEVSELDIAFHERLVQLADSPRLTRIHRTFITET
                     RMCIHALDETYSASEVRGEEHRAVANAIKSGNLELTDQLLIAHMDDAIRRLNASDATG
                     DCA"
     gene            complement(6652..7371)
                     /locus_tag="VFC01_02175"
     CDS             complement(6652..7371)
                     /locus_tag="VFC01_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleate cis-trans isomerase"
                     /protein_id="HZN78484.1"
                     /translation="MVTVGLLYPGHSAEDDFPALESRVGGAVRLPVVITSVGEDAHRV
                     DALLDLGGAKRLADGAAQLAAENPDAVMWACTSGSFVFGPEGARDQASGVAAAAGVPA
                     SSTSIAFVDAARHLGVRLVAVAASYPDDVAQHFVRFLKAGGVEVVAMGSHGIFTAAEV
                     GTLKPDQVVAMVTAADHPDAEAVLVPDTAMHTLAIISELEAAVGKPVLTANQVTVWKG
                     LPLLGPVPDLPGLGALFGDRR"
     gene            7418..>9868
                     /locus_tag="VFC01_02180"
     CDS             7418..>9868
                     /locus_tag="VFC01_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZN78485.1"
                     /translation="MIAAASYDVRQLARTLEAEIRGSVGADARSRALYATDASNYRVV
                     PDLVVVPADREDLATAVAMTAAAGAPVTLRGGGTSMAGNAIGGVVIDASRHVNRILAI
                     DADARTAVVEPGVVLTDLLAAGLPVGLAFGADPSSASRATLGGMIANNACGAHSVAWG
                     TTADNVRSLDVLLADGSRCTVDSVGNRDALARRPGREGELHRQLQAFVDRHELVIRRR
                     FGQFTRQISGYALHRLLPENGYDVAGLLCGSEGGFATTLQATVALTPLPAAKVLCVLG
                     FGDSITSAECVPVVLRHDPLTMESINIDLVERLPDEVRAAAIEAGLPAGRAWLLVEMG
                     GGDHASAVVAAEKMLEELRDSGSPATASLVTDPGAQRVLWRCRTDAAGLATRRPDGAE
                     AWGGWEDAAVPPARLSDYLRGLDELLGRYGFSGAYYGHFGEGCMHMRIDFDLLSRPGL
                     AIYREFVEQATDLVVSLGGSVSGEHGDGRVRSELLGRMYGEDGLRLLAEMKDIWDPRR
                     LMNPGVIVDPPALDAAIRHDGPAKDRKPLTLFAYADDNHDFAQAQRRCVGIGKCRQNS
                     GGVMCPSYQVTREELHSTRGRAHLLWEMLQGDVVTDGWRSTEVRDALDLCLSCKGCLS
                     DCPVNVDMATYKAEFTHHHYARRPWARPLSHWSMGWLPLSSRFASRAPRLVNRLARLP
                     LTKRLGGIAPEREIPAFAGQTFTSWFADHSPAEGAKGKVLLWPDTFTNHLAPEVGRAA
                     VEVLEAAGYQVVLPAKTVCCGLTWISTGQLTRAKKVLARSLAVLEPSLAAGTPIVGLE
                     PSCTAALRHDAPALLPDNP"
BASE COUNT         1516 a         3242 c         3407 g         1703 t
ORIGIN      
        1 ccagccgggt gccttggggt cgccgccctt cgatgcgacc accagatagg tatcgccgtc
       61 cttggcgtac gtcagcgaat tggtgcgatg gatgccggtc ttggctccga cggtgtgcag
      121 caggaggttg ggcggagtgc cgggcaggat gcggtgtccg atccggccgt tcgtgccctt
      181 gtagatcttg tcgtgcagca tcagcacggg caggaggatg tacttctcga ccggaggcat
      241 gccactcatg aagctcagtc ttgcgtagcc gagtccagtt ccgccagcgc ctcgcgcagc
      301 agccggccgg actcgtcacg gtccgggtcc cgtcgcagca gcatcccttt ggcgaacgac
      361 agcttgtcgc cattctgccg cggcagaacg tgcaggtgga tgtggggtac cgactggaag
      421 gcggcctggc catcgttgat gacgacattg ttgccgtcgg cgtgtagccc ggactggcgg
      481 gcggcccggg caatgcggtg gccgatgcgc accagcgcgg cgaccgtgtc cggcggggtg
      541 tcggtcaggt cgacggtgtg gcgcttggga atgaccaggg tgtggccgcg ggtgaacggt
      601 cgaatatcga ggatggccag gtagtcgtcg tcctcgtaga tccggatggc cggggcctca
      661 tcggcgacga ttgcgcagaa cacacaggac atcccgccac ggtatcgctc aaccgccgac
      721 gttctccttc gcccactccg ccatccggcg gtcgcgttcc tctccggaga cgtcctcgac
      781 gcgggtcatc acggtccagc gctggccgaa cgggtcgagg atcgacgcga accgatcgcc
      841 ggtgacaaac gtctgcgcgg gttcgcgaat ggtggctccg gcctgctcgg ccttggcgac
      901 gacagcgtcg acgtcctcgc agtagtacgc gacggaatgg gtggcgggcg ccgtgggatc
      961 gggcgcggcg atgtgatagg cctcagcagg gtcgccgagt tgcaggcgcc cgttgccgaa
     1021 gtccagttcg gcgtgcgcca ccgtgccgcc cgcgccgtcc atccggtcga cgaccttggc
     1081 gccgaacacg gttgcgtaga agtcgatggc cgccgcggca ccgtcgatgc acaggaacgg
     1141 ggtcaggctg gtgtagccct ccggaatggg tttgacgctc atgtctgcca gcttcggcga
     1201 ccgtgcggtg gccgtattgg agaaacgcga catggctacg ctggcccgcg tgcatgacga
     1261 cctgaacctt gacgatgaat tcgcggacga gtccgaatcc gaagatgcgg cattggagga
     1321 cgaaaacgcc catacgcttt cgattcgtcg cgcaaccggc gaagaggtgc tgagcgtctt
     1381 ctcgccgcac gaggagtgga gcgtctatct acagccgggc ggcggcatca cgaacgcgtt
     1441 cgtcggcaca ccgggcgaac ccattgtctg ggtcaacgcc aatgatcgtc gggtcgagcg
     1501 ggacgaagac ggcacgtacg tcatccgtat cgactagccc acgacgcctg gccgcctcac
     1561 agctgggctt gaccacgttg taacccccgc cgccgccgtt tgtgggcgat cggcagcggg
     1621 aagtcaaccg gtggccaaca gtcacctctt gctgttcaca actgaacgtg gcaatcttca
     1681 gattgtcaac aaaactacga cctgactctt gcggtgttgg tcacaccccg ctcgtgagat
     1741 gaccggcggg attcagggtc gatggacgac gaggaggatc ccgtggtctc cgaacaggct
     1801 caaccgccca ccgccgagcc cgaggcgccc ccgagtgtgg tgaggaaagc ggtcgccgca
     1861 tcggcgatcg gcaacgcgac cgaatggttc gactacggca tctacgccta tggcgtgacg
     1921 tatatatccg cggcgatctt ccccggggac accgcgaccg cgaccctgct cgcgctcatg
     1981 acgttcgcgg tctcgttcct ggtccggcca ctcggtggat tcgtctgggg tccgcttggt
     2041 gaccggctcg gccgcaagcg cgtgctggcg atcaccatct tgatgatggc cggggcgacg
     2101 ctgtgcgtcg ggctcgtgcc gagctacgcc gcgatcgggc tgtgggcgcc aatactgctg
     2161 gtgctgctgc gcatggtcca gggcttctcc accggcggcg agtacggcgg cgccgcgacg
     2221 ttcatggcgg aatacgcgcc gtgccggcgt cgtgggttcc tcggcagctt cctcgagttc
     2281 ggcacgttgg ccggattctc ggtcggagcg ctgctgatgc tcggattctc cacggtgctc
     2341 ggtgacgacc agatgcacgc atggggttgg cggctgccgt tcctggtcgc cgcgccactc
     2401 ggcctcgtcg gcgtctactt gcgcacccgg ctagaggaca ccccgatatt ccgtgagctg
     2461 gaggccaagg gccagcagga acacgagacc acgaccgagt tcaaggatct gctcgccggt
     2521 tactggcggc cgatcctgcg gctgggtggc ctggttgtcg ccctcaacgt ggtgaactac
     2581 accctgctca gctacatgcc gacctacttg gagggcgaga tcggcctgtc tgccgaccag
     2641 tcgctggtcg tcccgatcat cggcatgctc acgatgatgg tgttcctgcc gttcgccggc
     2701 gggctctctg atcggatcgg gcgcaagccg ctgtggtggt tctcgctagc cggcctgttc
     2761 gttgccggcg tgccgatgtt cctgttgatg ggtacgggcc tagccggagc ggtgatcggc
     2821 ttcgccgttc tgggtctgct gtatgtgcct cagctggcga cgatttcggc gaccttcccg
     2881 gcgatgttcc ccacccatgt gcggtatgcc ggattcgcga tcgcatacaa cgtgtccacc
     2941 tcgctcttcg gtggcaccgc acctgcggtc aacgaatggc tcatcggtcg gacgggcgac
     3001 ggccttgttc cggcgtatta catgatggct gcctgcgtga tcggcgcaat cgctctgatc
     3061 acggtccctg agaccaccag atgtccgatc ggtggcaggg ccgtcccagg taccaaggag
     3121 gccccaccac cggtggcgta caacccggtt cctgccccgg cctgagcgct gggctcgtca
     3181 cccgtgggcg tcgtcaggtg acgacggcgc ccatggcatc ggcccagatg ccggccgctt
     3241 catcgacctc gtcggcgttc acgacaagtg cggggatgaa gcgcacgacc tgactccacg
     3301 caccgcaggt caacagcagc agcccccgct tggcagcctc ctgctgaacg gcagcggccg
     3361 tcgtcgggtc gggtttgccg tcggcgtcgc gaaattcgtt cccgatcatc aggccgaggc
     3421 cacggacatc ggcgatctgg tcgtgcttgt ctgccaccgt ctgcagcgcc tgcttcagct
     3481 gcgctccgcg ctcggccgag ttctcgacca ggcgctcatc ttggatcacg tcgagcgtcg
     3541 caatggcggc ggcgcaggcc accgcgttcg cgccgtacgt gccgccctgt gatcccggcc
     3601 aggccttctc catcagtgct gacgacgccg cgatccccga cagcgggaag cccgacgcca
     3661 gccccttcgc cgtgaccacg atgtcgggcc gggcgttgaa atggtcaccg ccccagaacc
     3721 ttccggtccg gccgaacccg gtctgcacct cgtcgagcac caacaggatc ccgtatgcgt
     3781 cggcgcgctc acgcagcccg gcgaggaatc gctcgttcgc aggcacgtac ccgccctcgc
     3841 ccagcaccgg ctcgacgaag aacgcggccg tatcggctgg cgcgctcaag gtctgcagca
     3901 cgtagtcgag ctgggcgagc gcgaagtcgg tggcctgttc gacgggccag ccgaagtggc
     3961 cggggtcggg gaacggtgag acatgcacgc cacccatcag cggactgaat cccgaccgga
     4021 acttggtgcc ggaggtggtc atcgaggccg caccgatcgt tctgccgtgg aaaccaccgt
     4081 ggaagacaat gacattcggg cggcctgtcg cctggcggct gagccgcagc gctgcctcga
     4141 tcgcctcgct gccggaattc gcgaagaaca tccggtccag gcccggtggc agcacctcgc
     4201 cgagccgctc gaccaaggtg agcagggggc ggtgcatcac ggtggtgtac tgcgcgtgga
     4261 tcagcgtcgc cacctgtcgc tgcgccgctt cgaccacccg ggggtggcag tgcccggtgc
     4321 tcgtcactcc gatcccggcg gtgaagtcga ggtaccggcg atcgtcttcg tcgtagagct
     4381 gaacgccctc tccacgagca gcgagcacac cggttgcctg cttgaggatc tgcgagagtt
     4441 cggccacgac tttccccttg ccgtatcgat gggtagattg tcaacaatca gagtatcgga
     4501 caggcactgc ggggtggaag gggctacgga tgagcgagca cgaggcccag gtggtgaaag
     4561 ccgtcgaaaa gcggctgttc atcgacggca agtgggtcga cgcggccgac ggcgggacct
     4621 tcgacgtcat cgacccggct accggagcga cgctgtgcgc ggttgccgac gccacgcccg
     4681 ccgacggtag ggcggcgctc gatgccgccg tcgccgcgca gcccggcttc gccgcgaccg
     4741 cgccgcgcga acgcgccgac atgctcaccg gggcgttcga cctcctgcat gagcgaatcg
     4801 acgatttggc cctgctgatg accctggaaa tgggcaagcc gctagccgag gcccgcggtg
     4861 agatcgctta cgcggcagag tttttccgcc acttcgcggg cgaagcggta cgaatcgacg
     4921 gcggctacca gaccgcgccc gccggcggcg cacgattcct ggtcacccgt cagccggtcg
     4981 gaccgtgctt gctgatcacg ccgtggaact tcccgatggc gatgggcacc cgcaaactcg
     5041 gccccgcaat tgcggccggc tgcacgagtg tcgtcaaacc ggcccaccag acgccgctgt
     5101 cgatgctcgc gctaatggac atcctgaccg aggcaggcgt cccggccggc gcggtcaact
     5161 gcatcaccgc gatggatgcg ggcgcggtga tggagccgct tatccggtcc ggcctcgcgc
     5221 gaaagctctc cttcaccggc tcgaccagga ccggccgact gctgctcgag cagtgcgcgg
     5281 aaaagatcct gcgcacctcg ctagagctcg gcggcaatgc gccgttgatc gtgttcgagg
     5341 acgcggacct cgacgaagcc gtcgccgggg cgatcgccgc caagatgcgc aacatgggcg
     5401 aggcctgcac cgcggccaac cgggtctatg tgcactcgtc ggtcatcgac gagttcggtc
     5461 gtcgcctggc cgagcggatg gcggcgatgc gggtcggcag ggggacggaa gacggggtcc
     5521 aggtcggacc cttgatcgac gaggccgcac tgcagaaggt ggagtccctc gtcgccgacg
     5581 cggttgggcg cggcgccacc gtcctaaccg gcggctcggc gtccccaggc gacggctact
     5641 tctacccgcc gacggtgctc accggcgtac cccgcgacgc cgatatggcc gaccaagaga
     5701 tcttcgggcc cgtcgctcca ctcaccccct tcgacacgga ggcggaggtt ctcgcggcgg
     5761 ccaacgatac cgaatacggt ttggtctctt acgttttcac caacgacctg cgccgcgcac
     5821 tccgcgtcgc cgaggcactg gagacgggca tggtcggcct caaccagggc gtcgtctcca
     5881 acccggccgc gccgttcggg ggcgtcaagc aatccggcct cggccgtgaa ggcggcgccg
     5941 tcggcatcga cgagttcttg gagaccaaat acgtcgggat cgcgatgaac taggcgcagt
     6001 cccctgtggc gtcactggcg ttcaatcggc ggatggcgtc atccatgtga gcgatcagca
     6061 gttggtcggt gagttccaag ttgccggact tgatcgcgtt ggccaccgcg cggtgctcct
     6121 cgccgcgcac ctctgatgcg gagtaggtct cgtcgagcgc gtgaatgcac atccgcgtct
     6181 ccgtgatgaa ggtccggtgt atccgggtca ggcgcgggct atcggccagc tggacaaggc
     6241 gttcgtggaa ggcgatgtcc agctcgctga cttcggccgg cgtggtcgcg gccgccatct
     6301 gctcgacgat ctcgagcagc gcgtcgcccg ccgccacgta gtcgccctcg atgatcttgt
     6361 gggcggcggc gcgttcgatc gcctcgcgcg ccaggtacat atcgcggacg tcggcggtcg
     6421 tcatctcgat cacgaacaga ccgcgatggc ggatcgagac gagcaggcct tcttgggtga
     6481 gtcgctgcat gccctcgcgc agcgggccgc ggctgacccc gagtttgcgg gccagctcgg
     6541 cttcgctgag ttgtgcgccg ggcttcagtt cgccgtggcc gatcgcctgg cgcagcttct
     6601 ccgcgatgat gctcggcgtc gactgctgca tcagcggttc gatgtagctc gtcatcgccg
     6661 gtctccgaac agcgcgccca accccggcag gtcaggcacc ggtccgagga gcggcaggcc
     6721 cttccagacg gtcacctggt tcgcggtcag caccggcttg ccgaccgccg cctccagctc
     6781 ggagatgatc gccagcgtgt gcatcgcggt gtccgggacc agcacggcct ccgcgtccgg
     6841 gtggtcggcg gccgtgacca tcgcgacgac ctggtccggc ttcagggtgc cgacctcggc
     6901 cgcggtgaag atcccgtgac tgcccatcgc caccacttcg acgccgccgg ctttcagaaa
     6961 ccggacgaag tgctgagcca cgtcgtcggg gtaggacgcg gccacggcga ctaggcgcac
     7021 gccgaggtgg cgcgccgcat cgacgaacgc gatcgacgtg gacgacgccg ggacgccggc
     7081 cgcggcggcg acacctgacg cttggtcgcg ggcgccctcg ggcccgaaga cgaagctgcc
     7141 cgacgtacag gcccacatca ccgcatcggg attctcggcg gccagctgcg cggcgccgtc
     7201 ggccagccgc ttcgcaccgc cgaggtcgag cagggcgtcc acccgatgcg cgtcctcacc
     7261 gacggaggta atcaccaccg gaagccggac cgcgccgccg acccgggact ccagcgctgg
     7321 aaagtcgtcc tctgcgctgt gacccgggta gaggaggccc actgtgacca tgttgtctcc
     7381 ctagctgatc ggaatcagga tcggtagatt gttgacaatg atagccgccg cctcctacga
     7441 cgtccgccaa ctcgcgagga cgctcgaggc ggagattcgc gggtcggtcg gcgccgacgc
     7501 ccgcagtcgc gccctgtatg cgacggatgc gtcgaactac cgcgttgtgc ccgatctggt
     7561 cgtcgtgccg gccgatcgag aggacctggc cacagcggtg gcgatgactg cggcagcggg
     7621 cgcaccggtg acccttcgcg gcggtggcac gtcgatggcc ggcaacgcga tcggcggcgt
     7681 ggtcatcgat gcctcgcggc acgtcaaccg gatcctggcc atcgacgccg acgctcggac
     7741 cgcggtcgtc gagccgggcg tggtgctcac cgacttgctt gccgcggggt tgccggtcgg
     7801 actggcgttc ggcgccgacc cgtcatctgc gagccgggcg acgctcggcg gaatgattgc
     7861 gaacaacgcc tgcggcgcgc actccgttgc ctggggcacg acggcggaca acgtccgatc
     7921 gctcgacgtg ctgcttgccg acggcagccg ctgcacggtg gattccgtcg gaaaccgcga
     7981 cgcgctcgcc cgtaggcccg ggcgggaggg cgagctgcac cggcagctac aggccttcgt
     8041 cgaccggcac gagttggtca tccgccggcg gttcggacaa ttcacccgcc agatttccgg
     8101 ctacgcgttg catcggctgc tgccggaaaa cgggtatgac gtcgccgggt tgctgtgcgg
     8161 cagtgagggt ggtttcgcga cgacgctgca ggcgacggtt gcgctgacgc cgttgcccgc
     8221 ggcaaaggtg ttgtgcgtgc tcggctttgg cgactcgatc acgtcggcag agtgtgtgcc
     8281 ggtggtgctg cggcacgacc cgctgacgat ggagtcgatc aacatcgatc tcgtcgagcg
     8341 gcttcctgac gaggtccgcg ccgccgcgat cgaggcaggt ctgccggcgg ggcgagcctg
     8401 gttgctggtg gagatgggcg gtggggatca cgcgtcggcc gtggtggccg ccgagaagat
     8461 gctcgaggag ttacgcgatt ccggttcgcc cgccaccgcg tcgctcgtga cggatcccgg
     8521 ggcgcagcgg gtgttgtggc ggtgccggac ggatgcagcg gggctggcga cgcggcggcc
     8581 cgacggggca gaggcgtggg gcggctggga ggacgctgcg gtcccacccg cgcgcctgag
     8641 cgactatctg cgtgggctcg acgagctgct gggccggtat ggattctccg gggcgtatta
     8701 cgggcatttc ggtgagggct gcatgcacat gcggatcgat ttcgatctgc tcagccggcc
     8761 cggcctcgcg atctatcgcg aattcgtcga gcaggcaacg gatttggtgg tctcactggg
     8821 cggctccgtt tccggcgagc acggtgacgg gcgggtgcgc tcggaactgc tcggccgcat
     8881 gtatggcgag gacgggctgc ggctgctggc cgagatgaag gacatctggg atccgcgcag
     8941 gctgatgaat cctggtgtca tcgtcgatcc gcccgcactg gacgctgcga tccggcatga
     9001 cggcccggcc aaggaccgaa agccactgac actcttcgcc tatgccgacg acaaccacga
     9061 cttcgcgcag gcccagcgac gctgtgtcgg aatcggcaag tgccggcaga actctggcgg
     9121 tgtgatgtgc ccgagctatc aggtcacccg cgaggaactg cactcgactc gcgggcgcgc
     9181 ccatctgctc tgggagatgc tccaaggcga tgtcgtcacc gatggctggc ggtcgaccga
     9241 agtgcgggac gcgctggacc tgtgcctgtc gtgcaaagga tgtctgtcgg actgccccgt
     9301 caatgtcgac atggcaacgt acaaggccga attcactcac caccactacg cccgccgacc
     9361 ctgggcccgc cccctgtcgc attggtcgat gggctggctg cctctgtcgt cacggttcgc
     9421 ctcccgggcg ccccggttgg tcaaccgact ggcgcggctg ccactgacaa agcgattggg
     9481 cggcatcgcg ccggagcggg agatccccgc tttcgccggg cagaccttca cctcatggtt
     9541 cgccgaccac agcccggcgg aaggtgcgaa aggcaaggtg ctgctgtggc ccgacacctt
     9601 caccaatcac ctggcacccg aggtcgggcg ggctgcggtg gaggtgctgg aggccgctgg
     9661 ttatcaagtt gtgttgccgg cgaagacggt gtgttgcggg ctgacctgga tttccaccgg
     9721 ccagctgacg agggccaaga aggtgctcgc gcgctcgctg gcagtcttgg agccgtcgct
     9781 agccgccggg acgccgatcg tcgggttgga gccgagctgt actgcggcct tgcggcacga
     9841 tgctcccgcg ctgctgccgg acaaccca
//
LOCUS       DASRJA010000052         3086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2202300,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000052 DASRJA010000000
VERSION     DASRJA010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202300"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="VFC01_02185"
     CDS             <1..1220
                     /locus_tag="VFC01_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN78486.1"
                     /translation="REVDPSLLVTDSQIGAVQIVDATGTVLAASNGAPQSPLATPSLR
                     DGQARSVGRVEDRAAGFDYWVSARATAVPGGSVTILVGADREPVESVVTKVAALVAFG
                     SPVIIALMVVGTYRLVGAALGPVEAIRSRVASISSTDLAQRVPIPATQDEVAHLAATM
                     NAMLARLQRGRAAQHRLVSDASHELRSPLATITAALEMASERPELMDNELIDESLLPE
                     AHRMGQLIEDLLLLARSDEDAIELRRDDVDIDDLLLAEASRLRAIGSVHTVTHIQACR
                     AVGDRAALARVIRNLVDNAVRHASSTVTMDCRPEAAHAVITIADDGPGIPVQDRARVF
                     ERFVRLDPTRTRSSGGSGLGLSIVEQIVRSHHGTVAVGDAANGGAVFTLTLPLAQQDS
                     AGTDFQAGSLRSR"
     gene            complement(1193..1870)
                     /locus_tag="VFC01_02190"
     CDS             complement(1193..1870)
                     /locus_tag="VFC01_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN78487.1"
                     /translation="MRVLLVEDESRLAETVRRGLAAEGFVVDVEHDGVGGLAAAATGD
                     FDVVVLDIMLPGLSGYQIVRQLRTRQVWTPVLMLSAKDGEYDLADAFDIGADDYLTKP
                     FSFVVLVARLRALLRRGAPERPTVLTAGTLSLDPARRQVTRGQTALDLTPREYGVLEF
                     LLRNKGDVVSKSEILRSVWDSNYEGDDNVVEVYIGYLRRKIDVPFGVVTIETVRGAGY
                     RLRSDPA"
     gene            1982..2902
                     /locus_tag="VFC01_02195"
     CDS             1982..2902
                     /locus_tag="VFC01_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZN78488.1"
                     /translation="MSDVRAAILDRIWDAIAGPATASTFMTVLIVAAFVLGAQVLSEH
                     RQVAAALRVAGLLTVLTGLMLAIHRPGWVTELDTATASWVVAHRSTGFDVAALVISDL
                     GSPVATAAAALICAALLSWRARSRVPGIVVIGTVSAAALASTALKAVVDRPRPPLQWL
                     VLETDPSFPSGHVTGTAALLGITAVCVGMGRSRTVRAWLAGGVVTGVLVIAATRLYLG
                     VHWLTDVTAGAILAAVFVTIGTTVFAALHDRSGQHDAPPVAASTGEDPPVTTFGAGLI
                     NPATPIAADSHGVPSVRSHDMCEPHLERKS"
     gene            2899..>3086
                     /locus_tag="VFC01_02200"
     CDS             2899..>3086
                     /locus_tag="VFC01_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78489.1"
                     /translation="MRNPTRTLAVVAAVLATAAVLGGCGASGTAPSGSATLSSTTAAA
                     PAPETNPAGDIPDNQAFVP"
BASE COUNT          483 a         1097 c         1001 g          505 t
ORIGIN      
        1 cccgggaggt ggatccgtcg ttgttggtga ccgacagcca gatcggtgca gtgcagatcg
       61 tggatgccac cgggacagtg ctggccgcct ctaatggtgc accccaatcc cctttggcca
      121 ccccgtcgtt gcgcgacggg caggcgcgca gcgtcggacg ggtcgaagat cgcgcggctg
      181 gcttcgacta ctgggtgtcg gcccgtgcga ccgcagttcc aggcgggtcg gtgaccattc
      241 tggtcggcgc cgaccgggaa cctgttgaat ccgtcgtcac caaggtggct gccctggtcg
      301 cgttcggttc accggtcatc atcgcgttaa tggtcgtggg cacctaccgg ctggtggggg
      361 cagcgttggg accggtcgaa gcgatccgtt cccgggtggc gtcgatctcg agcaccgatc
      421 tcgcgcaacg ggtgccgatc ccggccaccc aagacgaggt cgcccacctt gcggcgacga
      481 tgaacgccat gctcgcccga ctgcaacgcg gccgcgccgc ccaacaccgg ctggtcagcg
      541 acgcttccca tgagctgcgc agcccactag ccacaatcac cgccgcactc gagatggcgt
      601 ctgagcggcc cgaactgatg gacaacgagc tgatcgacga atccctgctg cccgaagcgc
      661 accgcatggg tcagctaatc gaggatctac tgttgctggc acgctcggat gaagacgcga
      721 tagagctacg ccgcgacgac gtcgacatcg acgatctact cctggccgag gcgagccggc
      781 tacgcgccat cggatcggtc cacaccgtca cccacatcca ggcgtgccgc gccgtcggcg
      841 atcgcgccgc cctggcccga gtgatccgca atcttgtcga caacgccgtg cggcacgcct
      901 ccagcacggt caccatggat tgccgccccg aggcagctca cgcggtcatc acgatcgccg
      961 acgacggacc tggtatcccg gtgcaagacc gggcacgggt gttcgaacga ttcgttcgcc
     1021 tcgaccccac ccgtacccgg tcgtcggggg gatcgggcct cggtttgtcc atcgtcgagc
     1081 agatcgtgcg gtctcaccac ggcaccgtcg cggtgggcga cgcggccaac ggtggcgcgg
     1141 tctttaccct cacactgcca ctggcacagc aggactcggc cggcacagat tttcaggcgg
     1201 gatcgcttcg gagtcggtag ccggccccac gcacggtttc tatggtgacg acgccgaacg
     1261 ggacgtcgat cttgcgccgc agatagccga tatagacctc gacgacattg tcgtcgcctt
     1321 cgtagttcga atcccacacc gaccgtagga tttcggactt ggagaccaca tctcccttgt
     1381 tgcgtagaag gaattccagc acgccgtact cacgcggagt caggtcgagc gcggtctggc
     1441 cgcgggtcac ttgtcgacgt gccggatcca gcgacagggt ccccgcggtc agcacggtcg
     1501 ggcgttcggg ggcgccgcgt cgcaacaggg cacgcagtcg cgccacgagg accacgaagg
     1561 agaacggctt cgtcaggtag tcgtcggcgc cgatgtcgaa cgcgtccgcc aggtcgtatt
     1621 cgccgtcctt ggccgaaagc atcaggaccg gtgtccacac ctgacgggtt cgcaactggc
     1681 gaacgatctg atatccgctg agcccgggca gcatgatgtc gagcaccacc acatcgaaat
     1741 ctcccgtcgc ggcggccgcc aaaccgccca cgccgtcgtg ttcgacgtcg acgacgaatc
     1801 cctcggcggc caggccgcga cgcaccgtct cggccagtct ggattcgtcc tcgaccagca
     1861 gaacccgcac gactcacctc catctccgcc tcatctaccc ggtgaccagt agagcagaat
     1921 cctgggtggg cagcgatggc tcctcagcgt ctccacagcc accggacgcc aggctgttct
     1981 catgtccgac gtcagagccg ccatcctgga caggatctgg gacgcgatcg ccggcccggc
     2041 taccgcgtcg acgtttatga ccgtgctcat cgtcgctgcc ttcgtcctcg gtgcccaggt
     2101 gctatccgag caccggcagg tcgccgctgc cctgcgcgtc gcggggctgc tcacggtcct
     2161 gactggactc atgctcgcga tccaccgccc cggatgggtc accgaactcg acacggcgac
     2221 cgcgtcctgg gtggtcgcgc accgatccac ggggttcgac gtggccgcgt tggtgatcag
     2281 cgacctgggc agcccggtcg cgaccgcggc cgccgcactg atctgcgcgg cgctgctgtc
     2341 ctggcgggcc cggtcgaggg tcccgggcat cgtcgtgatc ggcacggtct ccgccgcggc
     2401 gctggccagt accgcactca aagcggtggt cgatcggccc cggcctccgc tgcagtggct
     2461 ggtcctcgag accgacccgt cgttcccgtc cggtcatgtc accggcaccg ccgcgctgct
     2521 cggcatcacc gccgtctgcg tggggatggg tcgcagccgc accgtacggg cctggctggc
     2581 gggtggggtg gtcaccggcg tgctggtcat cgccgccacc cgcctctacc tcggtgtgca
     2641 ctggctcacc gacgtcaccg cgggggcgat cctggccgcg gtgttcgtga ccatcggcac
     2701 cacggtgttc gcggctctgc acgaccggtc gggccaacac gacgcgccgc cggtcgccgc
     2761 gtcaaccggc gaagacccac cggtgacgac gttcggcgcc gggctcataa accccgccac
     2821 cccgatcgca gcggacagtc acggcgtgcc ctcggtgcgc tcgcacgaca tgtgtgaacc
     2881 gcacctggag cggaagtcgt gagaaacccc acccgcacgt tggcggttgt cgcggcggtg
     2941 ttggccacag cggcggtgct cggcgggtgc ggggccagcg ggaccgcccc gtcaggctcg
     3001 gccaccctca gcagtactac tgccgcagcg ccggctcccg agaccaaccc ggctggcgac
     3061 atccccgaca accaggcgtt cgtgcc
//
LOCUS       DASRJA010000053        13147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3609374,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000053 DASRJA010000000
VERSION     DASRJA010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3609374"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFC01_02205"
     CDS             <1..371
                     /locus_tag="VFC01_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016692752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN78490.1"
                     /translation="VNTGIFLLIIATFVLAYGVVQNSLAGAQGAWFPELFTANTRASG
                     ASLAYQISAMVSGFTPFITTLLFVSFGWIGPALLFSTYAAIGLVAALVTRETWGPRER
                     QLADEAAAAQPNPSDIPLPA"
     gene            383..1246
                     /locus_tag="VFC01_02210"
     CDS             383..1246
                     /locus_tag="VFC01_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZN78491.1"
                     /translation="MTLTIETGRRQKVAAAAYRARHHALNMGEAQGQGYIGQALGAAD
                     MLASVYADQLRCRPAEPEWEDRDRFLLSTGHYAIGLYAALAEAGIVSVEELDSYGCDD
                     SRLPMSGMASYTPGMEISGGSLGHGLTVAVGIAIGLRHQGSTSKVINFLSDGELDEGS
                     TWEAAMGASHHRLGHLTAMVDINALQADGPTAGVLRTEPVADKWAACGWFVQRVDGND
                     VDELLAAFDAVNEKADINGTPGVILCDTKVGRGVTLLENREKAHFMRIEANEWQQCRE
                     QLTAGYLGSDK"
     gene            1243..2226
                     /locus_tag="VFC01_02215"
     CDS             1243..2226
                     /locus_tag="VFC01_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZN78492.1"
                     /translation="MTTSAPLRTSAMIASFADPGVRTTPAPFGHALVEAAEKDERIVG
                     LTADLGKYTDMHIFAQAFPARYFQMGMAEQLLFGAAAGMAETGLVPFASTYSVFAARR
                     AYDFICLDIAEPGLNVNIVGGLPGLTTGYGPSHQATEDVAIFRGIPGLTIVDPCDSVD
                     ITQAVPQLAAHDGPTYLRLLRGQVPTVLDEYDYTFELGKAKVVRDGTDVVFVSSGLMT
                     MRALLAAEELSAHNVDVAVVHTPTLKPFDADTVLAQIDSDRLALTLENHTVVGGLFET
                     VASEALRRGVRRQVTPVALPDEFLAAGALPTLHDRYGLSTARIVAAVLDRL"
     gene            2270..2938
                     /locus_tag="VFC01_02220"
     CDS             2270..2938
                     /locus_tag="VFC01_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN78493.1"
                     /translation="MSVEASSLLRLEKTSLREQALTALRRAITTGQLTPGTHLVETEL
                     SEALQISRGTLREAMRQLQQEGLISAGARGRLSVRHLDAKEIKNIFDVRAALESLAAH
                     DLAGREDRTAAVAELRKAVADMEKWAASNLEDRIEADLKFHRTMCHLTGNETLLHSWS
                     SLEGSIRMSIMFAGVDRALKNMDAKRHSDIVDAIESGDADKAAATVRDHMAGAATVLV
                     DGAA"
     gene            2956..4632
                     /locus_tag="VFC01_02225"
     CDS             2956..4632
                     /locus_tag="VFC01_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZN78494.1"
                     /translation="MWSAWTPGLAQFRGYQRDWLRGDLVAGLTVAAYLVPQVMAYATV
                     AGLPPVVGLWAALVPLAVYAVLGSSRQLSVGPESTTALMTATALAPLATGDPGRYAVL
                     AAAAALLVGAMCFVAGLVRLGFLADLLSRPVLIGYMAGVALIMIGGQLGKVTGVSVEG
                     DEFVDQIRSFVGGLDELHWPTVALAAAVLAVLLAFAWLTPRLPGPLIAILIAAVVVSL
                     FSLHRNGIKVVGDIPSGFPAPELPAIPAADLMALFIPAAGIAIVAFNDNVLTARAFAV
                     RHGQDIDANAELRALGVCNAGAGLMQGFPVSSSGSRTALADAVGGRTQVYSLVTLVFL
                     VAVMLFGRGVLAMFPTAALGALVVYAALRLIDVPEFKRLARFRRSEVLLALATTAAVL
                     VFGVLYGVLVAIGLSILELLRRMARAHDGVLGFVPGVAGMHDVDDYPEADLVPGLLVY
                     RYDAPLFFANAEDFRERAMAAVGDYPGRVEWFLLNAEANVEVDLTALDAVDQLRTELQ
                     RRGIVFAMARVKQDLRDSLGAAGLLDKIGEDRIFPTLPTAVEAYNDRDAS"
     gene            4669..5094
                     /locus_tag="VFC01_02230"
     CDS             4669..5094
                     /locus_tag="VFC01_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZN78495.1"
                     /translation="MSIEDNKNLVRRFYREIDAGNLDAMDELVAEDYVDHSPAPFPGI
                     DAPGREGLKQAFRLFWDATPGTHEIEDQIAEGDKVVTRLTARGTHRGDLPGIPATGNE
                     MCMTATVIHRVANGRLAEKWSDKDVLAMLQQLNVIPPLN"
     gene            complement(5091..5744)
                     /locus_tag="VFC01_02235"
     CDS             complement(5091..5744)
                     /locus_tag="VFC01_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78496.1"
                     /translation="MPGAPIRRSRGRPAGGGITAEQSKQVFLDAAERLFATVGYREST
                     MEVIAREAGYSRGSIYRHFPTRDSLVESLIQRTTQRHMTGILDRLSPTAGPLDILIES
                     MVIVATELVHDPLLRTISDESDEGTVGRMLANNTALVQMVEPVMAEMLSSDDGRQFRR
                     DLRPKDLAQYFISTSISMLLGVIPGTEDAQTVRRYIDVFVLPAIVAHPPKPRAVFPD"
     gene            complement(6109..6567)
                     /locus_tag="VFC01_02240"
     CDS             complement(6109..6567)
                     /locus_tag="VFC01_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78497.1"
                     /translation="MRPPATAMVRVDVRTVTLLPAGSTGAPSKVWIKPMTTFRTMDPT
                     TSTSVGARGAVVRSVTATVERADIATVVSVSWTAVCCGVDSAADASGSAAALDSAGVV
                     DVSLGDGAGLSASVADVASLVSDVAVVGWRRDAGSSVCGLLFFGAGRVAF"
     gene            6849..7073
                     /locus_tag="VFC01_02245"
     CDS             6849..7073
                     /locus_tag="VFC01_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78498.1"
                     /translation="MTLPTRFVLAGHSLGGSLVMGAAGDMVDNDNAAIADLAGVLLLD
                     SVDVNDTVPMALQKLTGVNYRPVYDISSER"
     gene            7083..7505
                     /locus_tag="VFC01_02250"
     CDS             7083..7505
                     /locus_tag="VFC01_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78499.1"
                     /translation="MYGKVGDELAAARPGQFNGVMLVGGRHIDAMQGGNPLLQLSQYF
                     IAGFSQPQNVEVVKTLAVGWVNDMFARTHDGIYGAPQQSIQIATAAGTATAVVLPFTS
                     TQSVQATPFDGLSALVLNVLQKFFVYEPLAGRADALTT"
     gene            complement(7463..8737)
                     /locus_tag="VFC01_02255"
     CDS             complement(7463..8737)
                     /locus_tag="VFC01_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN78500.1"
                     /translation="MSTPDGPTLQRRLGTFDAVTIGLGSMVGAGIFAALAPAAAAAGS
                     GLLIGLAVAAVAAFCNATSSARLAALYPQSGGTYVYGRERLGEFWGYTAGWSFVVGKT
                     ASCAAMALTVGYYVWPQWAHAVAVAAVVALTAVNYAGIQKSAMLTRAIVAFVLAVLAA
                     VVVVVLGFGDVDGSRLAFGEDVSVHGVLQAAGLLFFAFAGYARIATLGEEVRDPARTI
                     TRAIPIALGITLVVYALVAVAILSELGSAALASTSAPLADAVRAAGFPGMEPVVRVGA
                     AVAALGSLLALILGVSRTTLAMARDRHLPHALSAVHPRFKTPHHAEIVVGVVVAAVAA
                     VADVRGAIGFSSFAVLLYYAIANASAWTLEPEGRRHQTLRRRSIPFIGLIGCLVLAFL
                     LPVSSVLIGAAVVAVGVVAYVVRASARPASGS"
     gene            complement(8753..9094)
                     /locus_tag="VFC01_02260"
     CDS             complement(8753..9094)
                     /locus_tag="VFC01_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78501.1"
                     /translation="MHHYHHRAFYIGVVCVFLIAVGLIALNFPVFIDGFDQYGFQVKC
                     GTGYISDLSQAAAAAGEHNYVDQCETALMLRRLWTIPLVVVGLIVLVIVVGVAATVWG
                     RESAFGEDPVA"
     gene            complement(9107..9331)
                     /locus_tag="VFC01_02265"
     CDS             complement(9107..9331)
                     /locus_tag="VFC01_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78502.1"
                     /translation="MLGALRGLLEFQVTIGEVIVFAVVVGVPYLIVGAIWSSTHIAHL
                     QHMVGADLVVSYLGSIVSWPVLLFADVCMT"
     gene            9436..10671
                     /locus_tag="VFC01_02270"
     CDS             9436..10671
                     /locus_tag="VFC01_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78503.1"
                     /translation="MARTGPTRAEGVRRRPKDRKAQIARASAEAFSTLGYYGVSMETI
                     AKRVGISAAALYRHYSSKYELFRDAVLNLGQQLVDCTEFADEASGDPELTLRRLVSAL
                     IDTAMINRESGGLYRWEGRYLRGDDQATLNSQMRTVHHRIQQQLTAIRPDLTSRQRWV
                     LSTSALSVIGSIVDHRAKLPAIQVRALLADLADTVLAADLPGVADSVVEDRPPVAAAA
                     EPKYEALLNESMRLFNIKGYRDTSVEDIAAAVGMPASGIYRYFSGKSDILAAAFRRAA
                     DRLSAELSAIVGTVPDREEALNAVIDAYVARSFNRPELDYVYYTERLHMTPGDQKILR
                     NLQRANVESWVELVVSVRPQWTPGQARFAVHAAMSLVIDLGRLMRYENSAPTRAVVER
                     LVDLTLLGRYRLRAPLPAK"
     gene            complement(10638..10904)
                     /locus_tag="VFC01_02275"
     CDS             complement(10638..10904)
                     /locus_tag="VFC01_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78504.1"
                     /translation="MPESNSRLAKFAGLAVAGIGLSHFTSPQLFDGITKSAFPRNTRQ
                     HVYIHGGVETALGLGLSSRKTRPLAVVGTIDYLAYLAGNGARNR"
     gene            complement(10924..12408)
                     /locus_tag="VFC01_02280"
     CDS             complement(10924..12408)
                     /locus_tag="VFC01_02280"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN78505.1"
                     /translation="MTPELYDAVIVGAGFGGIGAAIQLKRMGYHNFVILDREDDLGGT
                     WHVNHYPGLAVDVPTTTYSYFFEPNPNWSRLFSTGTEIKQYADDIADKYDVRRHMRFS
                     TTVEGARWDEEAKNWRVALAGGETLNTRYLITATGFLSQPKTPDIPGVTNFEGKVIHT
                     AAWDDTYDPTGRRIGLIGTGATAVQLIPELAKKAGDLTVYQRTPIWVVPKLDIPFGER
                     AKRLFSRIPLTQRAIRWLTDSIYEVMINTAIIHHRYFRRLNIAAADLSKIHRFASIRD
                     KELRRRLTPDYDFGCKRPTFSNGYYRAFTKPHVHLQTDGIARIEADGIVNTDGSRTEI
                     DTLVLATGFDLWDANFPAIEIIGREGRNLGKWWRETRFQAYQGVTMPYFPNYLSLASP
                     FAFLGLNFFNTMEYQMRHMDRLFGELKRRGASTFEVTEAANTKYRDRMTELLGDSLWT
                     IGNCATSRSYYFNPQGEPTLLRPMSTRTAIKEASDYPLSDYQIA"
     gene            complement(12452..13045)
                     /locus_tag="VFC01_02285"
     CDS             complement(12452..13045)
                     /locus_tag="VFC01_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HZN78506.1"
                     /translation="MPYQTQAPPYSTPKQTNWWAVVSLIFGVIGGVLISVICGIVGLN
                     KAKQGQGGRGLAIGGLVLSGLWVLVGVAAVVFFMVIDKGTVTATDVKVGDCLKEIPDS
                     IRVLTVDTIDCDQPHAGEVFAVLMMPEGDFPGQSAIEEYQNKCEPALSSYAPDAITDN
                     SVQLYVLYPTEETWKQGDRAVTCVATLDPPRTGSLKG"
BASE COUNT         2277 a         4216 c         4376 g         2278 t
ORIGIN      
        1 tggtgaacac tggaatcttc ctgttgatca tcgcgacctt cgtgttggca tacggggtgg
       61 ttcagaactc gctggccggt gcccagggcg cgtggttccc cgagctgttc acggcgaaca
      121 cccgcgcgtc gggagcgtca ctggcctacc agatctcggc gatggtgtcg ggcttcacac
      181 cctttatcac cacgctgctg ttcgtctcct tcgggtggat cgggccggcc ctgctgttca
      241 gcacctacgc cgcgatcggt ctggtggcgg cactggtcac ccgcgagacc tgggggccac
      301 gcgagcgcca gctggccgat gaggccgccg ccgcgcagcc caacccgtcc gacattccgc
      361 ttccggcata agggcgacgc acatgacact caccatcgaa accggccgcc gccagaaggt
      421 ggcggcggcc gcctaccgcg cacggcacca cgcgctcaac atgggcgaag cacagggcca
      481 gggatatatc gggcaggcac tcggcgcagc cgacatgctg gccagcgtct acgccgacca
      541 gctgcgatgc cgtcccgccg agcctgagtg ggaggaccgg gacaggtttc tactgtccac
      601 cggtcactac gccatcgggc tttacgcggc actggcagag gcgggcatcg tctcggtgga
      661 ggagctcgat tcgtacggct gcgacgactc gcgcctgccg atgtcgggaa tggcctccta
      721 cacacccggc atggagatct cggggggctc cctgggccac ggcctgaccg tggcggtcgg
      781 catcgcgatc gggttgcgcc accagggatc tacatcgaag gtgatcaact tcctgtcgga
      841 cggggaactg gacgagggct cgacgtggga agccgcgatg ggcgcgagtc atcaccggct
      901 cggccacctg actgcgatgg ttgacatcaa tgcgctgcag gccgacggtc ccaccgccgg
      961 tgtattgcgg accgagccgg tcgccgacaa gtgggcggca tgcggctggt tcgtgcagcg
     1021 ggtcgacggt aatgacgtcg acgagctgtt ggcggccttc gatgcggtca acgagaaggc
     1081 ggacatcaac ggaacgccgg gagtcatcct gtgcgacacc aaggtcgggc gcggggtgac
     1141 cttattggag aaccgtgaga aggcgcactt catgcggatc gaggccaacg aatggcaaca
     1201 gtgccgcgag cagctgaccg cgggctacct agggagtgac aaatgacaac cagtgcgcca
     1261 ctgcgtacct cggcgatgat cgcgtcgttc gccgatcccg gggtgcgcac caccccggcc
     1321 ccattcggcc acgcccttgt cgaggcggct gagaaggacg agcggatcgt cgggttgacg
     1381 gccgatctgg gcaagtacac cgacatgcac atcttcgcgc aggcgttccc agcgcggtac
     1441 ttccagatgg gcatggccga acagctgttg ttcggggccg ctgctgggat ggccgaaacc
     1501 gggctggttc cgttcgcgtc gacctattcg gtgttcgccg cccgtcgggc ctacgacttc
     1561 atctgcctcg acatcgccga gccggggctg aacgtcaaca tcgtcggcgg tctgcccgga
     1621 ctcacgaccg ggtacgggcc gtcgcatcag gccaccgagg atgtcgcgat cttccgcggc
     1681 atccctggcc tgacgatcgt cgatccttgc gactcggtcg acatcaccca ggccgtaccg
     1741 cagttggcgg cgcacgatgg acctacctat ctgcggctgc tgcgtggcca ggtgccaact
     1801 gtgctcgacg agtacgacta caccttcgaa ctcggcaagg ccaaggtggt ccgcgacggt
     1861 accgacgtgg tcttcgtttc gagcgggctg atgacgatgc gcgcccttct tgccgctgaa
     1921 gaactgtccg cccacaacgt cgacgtcgcc gtcgtgcaca cgccgacgtt gaagccgttc
     1981 gacgcggaca ccgttctggc ccagatcgat tcggaccggt tggcgctgac gctggagaac
     2041 cacaccgtcg tcggcgggct gttcgaaacc gtggcatccg aggcgctgcg tcgcggcgtg
     2101 cgccggcagg tcacgccggt tgcactgccc gacgagttcc tggccgctgg ggcgttgcca
     2161 acgctgcacg atcgttacgg gctgtcgacc gcgcggatcg tcgccgcggt gctcgaccgg
     2221 ctgtagcatg actgtcgact tccaattgtt gacagtgaag gagcgttcga tgtctgtgga
     2281 agcgtcgtcg ctgcttcggt tggagaagac cagccttcgt gaacaggcgc tgaccgcact
     2341 gcgccgcgcg atcaccaccg ggcagttgac cccgggcacc cacctcgtgg agaccgagct
     2401 gtccgaggcg ctgcagatca gccgtggcac gctgcgcgag gccatgcgac agctgcaaca
     2461 ggaaggcctg atctcggcgg gcgcgagggg acgtctttcg gtgcgccacc tcgacgccaa
     2521 ggagatcaag aacatcttcg acgtccgcgc cgcgctggag tcgttggccg cccatgacct
     2581 ggctggccgc gaggaccgca cggcggctgt agcggagctg cgcaaggcgg tcgcggacat
     2641 ggagaagtgg gccgcgtcca acctcgagga ccgcatcgag gcggacctga agttccaccg
     2701 caccatgtgt catctaacgg ggaacgagac gctgctgcac tcgtggtcat cgctggaggg
     2761 cagcattcgg atgtccatca tgttcgccgg cgtggaccgc gcactgaaga acatggacgc
     2821 caaacggcat tccgacatcg tcgacgcgat cgagtccggc gacgccgaca aggccgccgc
     2881 caccgtccgc gaccatatgg ccggcgccgc aacggttttg gtggacggag cggcctgaga
     2941 ccgagggtcg acatgatgtg gtcggcctgg acgcccggac tggcgcagtt ccggggctat
     3001 cagcgtgact ggctgcgcgg tgacctggtg gccggtctga cagtcgccgc ctatttggtg
     3061 ccacaggtga tggcgtacgc gacggtcgcg ggtctgccgc ccgtcgtcgg tctgtgggcc
     3121 gcgctggtgc cgctcgccgt ctacgcagtg cttggctctt cgcggcagtt gtccgtcggc
     3181 ccggagtcga cgacagcgct gatgaccgcg accgcgctcg cgccgctagc cacaggcgac
     3241 cctggccggt acgcggtgct ggcggccgcg gctgcgctgc tcgtgggcgc aatgtgcttt
     3301 gtggcgggcc tcgtccggct ggggttcctc gccgatctgc tctcgcgccc ggtactgatc
     3361 gggtacatgg cgggcgttgc gctcattatg atcggcggcc aactgggcaa ggtcacgggc
     3421 gtctcggtcg agggcgacga gtttgtggac cagatccggt ccttcgtcgg tgggctggac
     3481 gaattgcact ggccgactgt ggctttggcg gcagcggtcc tcgcggtctt gctggctttc
     3541 gcgtggctca cgccgcggtt gccgggcccg ctgatcgcga ttctcatagc tgctgtagtc
     3601 gtcagccttt tctccttgca ccgcaacgga atcaaggtcg tcggcgacat ccctagtggt
     3661 ttcccggcac ccgaactgcc cgccatcccg gccgctgatc tgatggccct gttcattccg
     3721 gctgcaggca tcgcgatcgt ggcgttcaac gacaacgtgt tgacggctcg cgcattcgcc
     3781 gtccgtcacg gccaggacat cgacgcgaac gcggaactgc gtgcgctcgg cgtgtgcaat
     3841 gctggcgccg ggctgatgca gggcttcccg gtcagctcca gcggaagccg tacggcactc
     3901 gctgacgcgg tcggtggccg cactcaggtc tactcgctcg tgacgctggt gttcctggtc
     3961 gcggtgatgc tgttcgggcg gggcgtactg gcgatgtttc cgaccgcggc gctgggcgcc
     4021 ttggtcgtgt acgccgcgct tcgcctgatt gacgtgccgg agttcaagcg gttggcgcga
     4081 ttccgtcgca gtgaggtgct tctcgcgctg gccaccaccg cggccgtgct ggtgttcggt
     4141 gtgctctacg gggtgcttgt cgccatcggc ctgtcgatac tcgaactgct gcgccggatg
     4201 gcgcgagcgc atgacggcgt gctcggcttc gtgcccggtg tcgcgggcat gcacgatgtg
     4261 gacgactatc cggaggctga tctcgtcccc gggttgttgg tgtaccgcta cgacgccccg
     4321 ttgttcttcg ccaacgccga ggacttccgc gaacgggcaa tggcggcggt cggcgactac
     4381 ccggggcgtg tcgagtggtt cctgctcaac gccgaagcca acgtcgaggt cgacctcacc
     4441 gcattggatg ccgtcgatca actccgcacc gaattacaaa ggcgtggaat cgtattcgcg
     4501 atggccaggg tgaagcagga tctgcgcgat tcgcttgggg cggcgggtct gctcgacaag
     4561 atcggcgagg atcgcatctt cccgacactg ccgaccgcgg tcgaggccta caacgaccgg
     4621 gacgcgtcct agcgcgtatc gtcggaatat ctcaggggga ggcaacgcat gtcgatcgaa
     4681 gacaacaaga acctggtccg tcgtttttac cgagagatcg atgccggcaa tctcgatgcg
     4741 atggacgaat tggtggccga agattatgtg gaccattcgc cggcaccctt tccaggtatc
     4801 gatgctcccg ggcgcgaggg gctcaagcag gcgttccggt tgttttggga cgcgacgccg
     4861 ggcacccatg agatcgagga ccagatcgcc gaaggcgaca aggtggtcac gaggctcacg
     4921 gcccgtggaa cgcaccgcgg cgatctcccc ggcatcccgg ccaccggcaa cgagatgtgt
     4981 atgactgcca cggtgatcca ccgtgtcgcg aacggccgac tggcggagaa gtggtcggac
     5041 aaggacgtgc tcgcaatgct ccagcaactg aacgtcatcc cgccactgaa ttagtcaggg
     5101 aaaacggcgc gtggcttagg cgggtgcgcc acgatggccg gcagcacgaa gacgtcgatg
     5161 tagcgacgca ccgtttgggc atcctcggtg cccggtatga cgccgagcag catgctgatt
     5221 gaggtggaaa tgaagtactg cgccaagtcc tttggtcgca aatcgcgtcg gaactgacgc
     5281 ccgtcgtcgg aggacagcat ctctgccatc acgggttcca ccatctgtac gagagcggtg
     5341 ttgttggcca gcatgcggcc gacggtgccc tcgtcgctct cgtcggagat ggtcctcagc
     5401 agagggtcat ggaccagttc ggtggccacg atcaccatgc tctcgatcaa gatgtcgagc
     5461 ggcccggcag tgggggacag tcggtcaagg atgcccgtca tatgccgctg cgttgtccgc
     5521 tgtatcagcg attccacgag gctgtctcgg gtcggaaagt ggcggtaaat gctcccccgg
     5581 gagtaaccgg cctctcgcgc gatcacctcc atcgtcgact cgcgatagcc gacggtggcg
     5641 aataaccgct ccgcggcgtc gaggaagact tgcttcgact gctcggccgt gatgccgccg
     5701 ccggcggggc ggccgcgact acggcgtata ggtgctcctg gcacatgccg acggtacccg
     5761 cgtgagcaga aaatgacata taccttgatt tgttcaattt ctgcggctag cgtcatcgcg
     5821 gtcgcatgga ggtgacatgg tcaatcggct gatggcgctg ttggggttgg cggtagtgct
     5881 agtggcgatc gcggcggcat tgctcgccgg agccgcgagt gcagcggcgg agaccggctc
     5941 agcatctgac ggcgacggca ccaaagcgtc tgaatcaaag catgagtcga ctcccaagtc
     6001 ggatcccgag acgaatgaga cgacaaggcc gaccggagag cagagttcga ccgagagcga
     6061 cagcgatacc accgacatcg gcaagcagga gaccagcggt tcggaggttc agaaggccac
     6121 gcgtcccgcg ccgaagaaca acaggccgca caccgacgag ccggcatcgc gtcgccaccc
     6181 aaccactgcc acgtcggaca ccaaggacgc cacatccgcg acggaggccg acaagcccgc
     6241 accgtctccc aatgaaacat ccaccacgcc agccgaatcc agcgccgcag cggagccgga
     6301 ggcgtccgca gcagagtcga cgccacagca gacagcagtc cacgagacgg agaccacagt
     6361 ggcaatgtcg gcgcgctcga ccgtcgccgt caccgaccgg accacggccc cgcgcgcacc
     6421 gacgctcgtc gacgtggtcg ggtccatcgt cctgaacgtc gtcatgggct tgatccacac
     6481 cttcgacgga gcaccggtgc tgccggcggg cagtaacgtc accgtgcgca cgtcgaccct
     6541 caccatcgcc gtcgcgggtg gtcgcactgt cgaagcggat tggtatttcc ccaacgacga
     6601 cgaaacacca acgcgcttca gttacttcca gcacggccta ggggcttctg ccccgatgta
     6661 cagctacacc gcggcgaccc tggccgagca gaccaacagc atcgtcgtcg ctccgtcgtt
     6721 gacttcgaac ttcttcgacg ccgacggcgc ctggctcggt ggcacgccga tgcaccaagc
     6781 agtcgccgac ctgttcgtag gggaacgcga agcgctgacg gagtgccagc gcggcagcgg
     6841 gatacgaggt gacattgccg acgcggttcg tgttggccgg acattcactc ggcggcagtc
     6901 tggtgatggg cgccgcaggc gacatggtcg acaacgacaa cgcggcgatc gcggatctgg
     6961 ccggagtgct gctcctcgat tcggtggatg tcaacgacac cgtgccgatg gccctgcaga
     7021 agctgaccgg tgtgaattac cgccccgtgt atgacatctc gtcggagcgg taggtgtgga
     7081 acatgtacgg aaaggtgggc gacgagttgg cggcggctcg acccgggcag ttcaacggcg
     7141 tcatgctcgt cggcggccgc cacatcgatg ccatgcaggg cggaaacccg ctacttcagc
     7201 tcagccagta cttcatcgcg ggcttctcgc agccgcagaa cgtcgaggtg gtcaagactc
     7261 tggcggtggg atgggtgaac gacatgttcg cccgcaccca tgacggcatt tacggtgcgc
     7321 cgcaacagag tatccagatc gccaccgccg cgggtacggc caccgccgtc gtgctgccgt
     7381 tcacgtcgac acagtcggtg caagctaccc cgttcgacgg gctgtcggct ctcgtcctca
     7441 acgtcctgca gaagttcttc gtctacgaac cgctggccgg ccgggctgac gccctcacca
     7501 cgtaggcaac cacgccgacg gcaacgacgg ccgcgccgat gagcaccgac gaaacgggca
     7561 gcaggaacgc cagcaccagg cagccgatca gtccgatgaa cggtatggac ctgcggcgca
     7621 gggtttgatg tcgccggccc tccggctcca gcgtccatgc cgacgcgttg gcgatcgcgt
     7681 agtacagcag caccgcgaac gacgagaagc cgatcgcccc acgcacatcg gccaccgccg
     7741 ccaccgcggc gacgacgact ccgaccacga tctcggcgtg atgcggggtc ttgaaccgcg
     7801 gatggactgc agacagcgcg tgcggcagat gccggtcgcg cgccatcgcc aacgtggtcc
     7861 gggatacgcc gaggatcagg gccagcagcg aacccagcgc ggccaccgcc gcgcccactc
     7921 gcaccaccgg ctccatcccg gggaagcctg ccgcgcgcac cgcgtctgcc agcggtgcgc
     7981 ttgtcgaagc cagtgcggcg ctgcccaatt cgctcagtat ggcgaccgcg accaatgcgt
     8041 acaccaccag cgtgatgcca agcgcaatcg ggatggcgcg cgtaattgtg cgcgccggat
     8101 cgcgcacctc ttcgccgagt gtcgcgatgc gggcataccc cgcgaacgcg aagaacagca
     8161 gtcccgcggc ttgcagtacg ccgtggacgc tgacgtcttc gccgaacgcc agccgcgagc
     8221 cgtcgacgtc accgaagccg aggacgacga ccaccacagc cgcaagcacc gccagcacga
     8281 acgcgacgat ggcgcgggtc agcatcgccg acttctggat gccggcgtag ttcaccgcgg
     8341 tcagcgcgac caccgcggcc accgccaccg cgtgcgccca ctgcggccac acgtagtagc
     8401 cgacggtcag cgccatcgcc gcgcacgacg ccgtcttgcc gacgacgaag ctccagccgg
     8461 cggtgtagcc ccagaactca ccgaggcgtt cgcgtccgta cacgtaggtg ccgccggact
     8521 gcggatacag cgcggccaac cgggccgacg acgtggcgtt gcagaaggcc gccacggcgg
     8581 cgaccgccag cccgatgagc agacccgacc cggcggctgc cgcagccggg gccagcgcgg
     8641 cgaagatgcc tgcgcccacc atcgagccga gtccgatcgt cacggcgtcg aacgtcccga
     8701 gccgccgttg cagcgtaggg ccgtccggcg tgctcacggc ggaaatgcta gttcaggcga
     8761 ctgggtcttc cccgaatgcc gattcacggc cccaaacggt ggccgccaca ccaaccacaa
     8821 tgacgagcac aatcaacccg accaccacga gtgggatcgt ccacaaccgg cgcagcatca
     8881 gcgctgtctc gcattggtcg acgtagttgt gctcgccggc cgcggccgcc gcctggctca
     8941 gatcgctgat gtaccccgtc ccgcacttga cctggaatcc gtattggtcg aagccgtcga
     9001 tgaacaccgg aaagttcaac gcgatgagac cgacagcgat caggaagaca caaacgactc
     9061 cgatgtagaa cgcgcgatgg tgatagtggt gcaaagcgtc ccccgttcag gtcatgcaga
     9121 cgtcggcgaa gagcaacaca ggccaggaca cgattgaccc caggtacgac accacgaggt
     9181 cggcgcccac catgtgttgc agatgcgcga tgtgcgtgct cgaccagatg gctcctacga
     9241 tcaggtacgg aacgccgacg actaccgcga agacgatcac ttctcctatc gtcacctgga
     9301 actccaacag ccctcgtagc gcgccgagca cgctgactat gttaacggcg attcttgtct
     9361 atgcgatcac ttttttgtga ttttccaacc gaagtgggcc gctatgtatg cgcgccgtcc
     9421 gttatggtgt tggccatggc gcgcaccggt cccacccgcg ccgagggtgt tcggcgccgg
     9481 ccgaaggatc gcaaggcgca gatcgcccgc gcgtcggccg aggcgttcag cacgctcggc
     9541 tactacggcg tgagcatgga gacgatcgcg aagcgggttg gaatctccgc tgccgcgctg
     9601 taccgccact actcgagcaa gtacgaactc ttccgcgacg cggtgctcaa cctcggtcaa
     9661 caattggtcg actgcacgga attcgccgac gaggcatccg gcgatcccga gctgacgttg
     9721 cggcggctgg tgtcggcgct catcgacacc gcgatgatca accgtgaatc gggcgggctc
     9781 tatcgctggg agggccgcta tctgcgcggc gatgaccagg ccacgctgaa ctcgcagatg
     9841 cgcaccgtcc accatcggat ccagcagcag ctcacggcaa ttcggccgga cctgacctct
     9901 cggcagcggt gggttctgtc gacgtcggcg ctgtcggtga tcggcagcat cgtcgatcac
     9961 cgcgccaagc tgcctgccat ccaggtgcgg gcgttactcg ccgacctcgc cgacacggta
    10021 ctggccgcgg acctgcccgg tgtggcggac agtgtcgtcg aggaccgccc gcccgtcgcc
    10081 gctgccgcag agccgaaata cgaagcgctg ctgaatgaat cgatgcggct gttcaacatc
    10141 aaaggctacc gcgataccag cgtggaggac atcgcggcgg cggttgggat gcccgcgtcg
    10201 ggcatctacc gttacttctc gggcaagagt gacatcctcg ccgcggcgtt ccggcgagcg
    10261 gccgaccggc tgtcggcaga gctgtcggcc atcgtcggca ccgtgcccga tcgcgaggaa
    10321 gcactcaacg cggtcatcga tgcgtatgtg gcgaggtcgt tcaaccggcc cgagctggac
    10381 tatgtgtatt acaccgagcg attgcatatg acaccgggcg accagaagat cctgcgcaac
    10441 ctgcagcggg ccaacgtgga gtcgtgggtg gagctggtgg tgtcggtacg gccgcagtgg
    10501 acgccggggc aggcgcggtt tgccgtccac gccgccatgt cactggtgat cgacctgggc
    10561 cggttgatgc gctatgagaa ttcggcgccg acccgtgcag tcgtcgaacg gctggtggac
    10621 ctgacgctgc tcggtcgcta ccggttgcgc gccccgttgc cagccaagta agccaagtaa
    10681 tcgatcgtgc cgacgaccgc cagcgggcgc gtcttgcgtg agctcagccc gagcccgagt
    10741 gcagtctcga cgccgccgtg gatgtagacg tgctggcgcg tgttgcgcgg aaaggcggac
    10801 ttggtgatgc cgtcgaacag ctgtgggctg gtgaagtgag acagcccgat gccggcgacc
    10861 gccagcccgg cgaacttggc gagacgcgaa ttgctttctg gcacagctgt tgatcctctc
    10921 aggtcacgca atctggtagt cgctcaacgg gtagtctgat gcctctttga tcgcggtgcg
    10981 cgtcgacatc ggccgcagaa gcgtcggttc gccctgcggg ttgaagtagt aggaccgcga
    11041 cgtcgcgcaa ttgccgatcg tccacagcga atcgccgagc agttcggtca tccgatcccg
    11101 gtacttcgtg ttggccgcct cggtcacctc gaacgtggag gcgccgcgcc gtttgagttc
    11161 accgaacaac cgatccatgt gccgcatctg gtactccatc gtgttgaaga agttcaaccc
    11221 gaggaacgcg aaaggactgg caaggctgag gtagttcggg aagtacggca tcgtcacgcc
    11281 ctgataggcc tggaaacgcg tttcgcgcca ccattttccg aggttgcggc cctcgcgccc
    11341 gatgatctcg atggcgggga agttggcgtc ccacagatcg aatccggtgg ccaataccaa
    11401 ggtgtcgatt tcggtcctgg agccatcggt gttgacgatg ccgtcggcct cgatgcgcgc
    11461 gatgccgtcg gtctgcagat gcacgtgtgg tttggtgaat gcccggtagt agccgttgga
    11521 aaacgtcggc cgcttgcaac cgaagtcgta gtcaggggtg agcctgcggc gcagctcctt
    11581 gtcgcggatc gacgcgaacc ggtggatctt cgacaggtcc gctgcggcga tgttgagccg
    11641 acggaagtac cggtgatgaa tgatcgcggt gttgatcatg acttcgtaga tcgaatcggt
    11701 cagccaccga attgcgcgct gcgtcaacgg gatccgtgag aacagcctct tggcccgttc
    11761 gccgaagggg atgtcgagtt tggggaccac ccagatcggt gtgcgctggt agacggtgag
    11821 gtcgccggcc ttcttcgcca actcggggat cagctgtacc gcggtggcgc ccgtgccgat
    11881 gaggccgatg cggcgccctg tcgggtcgta ggtgtcgtcc caggccgcgg tgtggatgac
    11941 cttgccctcg aagttcgtga cgcccgggat gtcgggtgtc ttgggctgcg acaggaagcc
    12001 ggtcgcggtg atgaggtagc gggtgttcag cgtctcgccg ccggccagcg cgacccgcca
    12061 attcttggct tcctcgtccc agcgcgcgcc ctcgaccgtc gtgctgaacc gcatatggcg
    12121 gcgcacgtcg tacttgtcgg cgatgtcatc ggcgtactgc ttgatctcgg tgccggtgga
    12181 gaacagccgc gaccagttcg ggttcggctc gaagaagtac gaataggtgg tggtggggac
    12241 gtcgacggcc aggccggggt agtggttgac gtgccaggtg ccgcccaggt cgtcttcgcg
    12301 gtcgaggatc acgaagttgt ggtagcccat ccgcttgagc tggatcgccg cgccgattcc
    12361 cccaaaaccg gcccccacga tgaccgcgtc gtaaagctcc ggagtcatga cccagacggt
    12421 acctcaggta ccggaaactt tgccgaccgg ctcagccctt cagagacccc gtgcgcggtg
    12481 gatccagggt ggcgacgcag gtgacggcgc ggtcgccctg cttccaggtc tcctccgtgg
    12541 gatacagcac gtacagctgc accgagttgt cggtgatcgc gtcgggtgca taggaggaca
    12601 gcgcgggctc gcacttgttc tggtactcct cgatcgcgga ctgcccaggg aagtcaccct
    12661 ccggcatcat cagcaccgcg aaaacctctc cagcgtgggg ttggtcgcag tcgatcgtgt
    12721 cgaccgtcag cacgcgaatg ctgtcgggta tctccttgag gcagtcgcca acctttacgt
    12781 ccgtcgcggt gaccgtgcct ttgtcgatga ccatgaagaa gacgacagct gcgacgccca
    12841 cgagcaccca cagccccgac agcacgaggc cgccgatcgc cagcccgcgc ccgccctggc
    12901 cctgcttggc tttgttcagc ccgacgatgc cgcagatcac gctgatcagg acgccgccga
    12961 tgacgccgaa gatcagcgag acgaccgccc accagttggt ctgcttcggc gtggaatagg
    13021 gcggcgcctg cgtctgatag ggcatcggcg ggggcggtgt cgggtatccg cctggcggtg
    13081 gtggcgggta tccgcctggc ggtggtggcg ggtatccgcc tggcggtggt ggcgggtatc
    13141 cgcctgg
//
LOCUS       DASRJA010000054         4885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1083368,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000054 DASRJA010000000
VERSION     DASRJA010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083368"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFC01_02290"
     CDS             complement(<1..339)
                     /locus_tag="VFC01_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZN78507.1"
                     /translation="MSFDVVEASIAQLRQALDDGQVTAVGLLESYLRRIAVYDHAGIR
                     LNAVVVLNPEAHADAVASDARRESSRTRGPLDGIPYTAKDSYLARGLTAAAGSPAFAD
                     LVAQKDAFVIE"
     gene            complement(340..1755)
                     /gene="hflX"
                     /locus_tag="VFC01_02295"
     CDS             complement(340..1755)
                     /gene="hflX"
                     /locus_tag="VFC01_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZN78508.1"
                     /translation="MTYPDFPINGTPSAGELALEDRSALRRVAGLSTELGDISEVEYR
                     QLRLERVVLVGVWTEGSAADADASMAELAALAETAGSEVLEGLIQRRDKPDASTYIGS
                     GKAQELREVVLATGADTVICDGELSPAQLTALEKAVKVKVVDRTALILDIFAQHATSR
                     EGKAQVALAQMEYMLPRLRGWGESMSRQAGGRAGGSGGGVGLRGPGETKIETDRRRIR
                     ERMSKLRRDIRAMKQVRDTQRSKRLASDVPSIAIVGYTNAGKSSLLNALTGAGVLVEN
                     ALFATLDPTTRRGEFADGRPFVLTDTVGFVRHLPTQLVEAFRSTLEEVVDADLLVHVV
                     DGSDVNPLAQINAVRQVLNDVIADHDGKPAPELLVVNKTDAADGLTLAQLRRALPDAV
                     FVSARTGDGVDRLQQRMAELITPTDTMVDVTIPYDRGDLVNKVHADGRVDATEHTAAG
                     TRIKARVPIPLAASLREFATF"
     gene            complement(1801..2661)
                     /gene="dapF"
                     /locus_tag="VFC01_02300"
     CDS             complement(1801..2661)
                     /gene="dapF"
                     /locus_tag="VFC01_02300"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZN78509.1"
                     /translation="MEFAKGHGTQNDFVLLPDPDGRLSLTPAAVAALCDRRRGLGADG
                     VLRVTRAEVAGLERIPEGVQPGDWYMDYRNADGSIAQMCGNGVRVFAHYLRANGLESR
                     DEFTVGSLAGPRPVVLHAWDDTTADVTVEMGKTSQLGTGEAIVGGRPFAGLAIDVGNP
                     HLACVDADLSFDELAALDVAAPVSFDRTQFPEGVNIEVLTAPHGGAVSMRVHERGVGE
                     TRSCGTGTVAAAVAALAFDGATTGTLQVRIPGGEVTVTVTDATSYLRGPSVLVARGEL
                     SEEWWRIAQR"
     gene            complement(2666..3571)
                     /gene="miaA"
                     /locus_tag="VFC01_02305"
     CDS             complement(2666..3571)
                     /gene="miaA"
                     /locus_tag="VFC01_02305"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZN78510.1"
                     /translation="MRPIAIIGPTGTGKSALAVAVAERVGGEIVNADAMQLYRGMDIG
                     TAKLAVPERRGIAHHQLDVLNVTETASVARYQQAATVDVEAIAGRGAVPIIVGGSMMY
                     IQSLLDEWSFPATDPAVRAKWEERLAEIGVGALHQELARVDAQAASSILPTDGRRIVR
                     ALEVVELTRQPFAASAPVIGTPRWNTAIIGLDWDTALLDSRLTQRTDKMFVDGLVGEV
                     QGLLGAGLRDGVTASRALGYAHVIADLDQGGDGSAAREPTFFGTRRYVRRQRSWFRRD
                     HRITWLDGAAADNVENTLQIWRHVS"
     gene            complement(3568..4251)
                     /locus_tag="VFC01_02310"
     CDS             complement(3568..4251)
                     /locus_tag="VFC01_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78511.1"
                     /translation="MLSAIAIIPSAPVMVPELGAGAADELADLREAVFTATGSLPSRW
                     IALGVGEADAVLDSETAGTLAGYGVDRRVTLSPNAAGTPTELPLCALIAGWVRDQAKP
                     DTSAEVRVYATYHDIDAAIASGRRLRAEIDEAVDPIGVLVVADGAHTLTPPAPGGYDP
                     DSIPVQAALDDALAAGDAAALTRLPDTVVGRVAYQVLAGLAGSPRSAKELYRGAPYGV
                     GYYVGVWRP"
     gene            complement(4268..>4885)
                     /locus_tag="VFC01_02315"
     CDS             complement(4268..>4885)
                     /locus_tag="VFC01_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN78512.1"
                     /translation="SLLFALPISARLSHRPVTRWEWTWAALLAAAVAIIVTVGNPTAG
                     HSRASYETWTAVIAVLGPALVLCVVGARIWSGSVSAVLLALVSGALWGVFAVLTKGVV
                     DRLDDGLWALLRTPELYVWALVGIAGTAWQQSSFRAGSLTASLPTMTVAEPVVGSALG
                     IVVLGETLRPGDAGWLTLIIAVAVMVVSTVALARGEAAGATKQPA"
BASE COUNT          772 a         1722 c         1634 g          757 t
ORIGIN      
        1 ctcgatcaca aacgcgtcct tctgggcgac caggtcggcg aacgccggac tgcccgcggc
       61 tgcggtgagc ccgcgtgcca ggtaactgtc cttggcggtg tacggaattc cgtccaacgg
      121 gccgcgggtc cggctgcttt ccctgcgcgc gtcggaggcc actgcgtcgg cgtgcgcctc
      181 cggattcagt acgacgacgg cgttcaatcg gatgcccgcg tggtcgtaga ccgcgatgcg
      241 ccgcaagtag ctttcgagca ggccgaccgc ggttacctgg ccgtcgtcca atgcctgccg
      301 cagctgcgcg atggacgctt cgacgacgtc gaacgacact tagaacgtcg cgaattctcg
      361 caggctggcg gccaacggga tcggcacgcg tgccttgatt ctggtgccgg cggcggtgtg
      421 ctcggtggca tcgacccgtc cgtcggcatg caccttgttc acgagatcgc cgcggtcgta
      481 cgggatcgtc acgtcgacca tggtgtcggt cggcgtgatc agctcggcca tccgctgttg
      541 cagccggtcc acgccgtcgc cggtacgtgc ggagacgaag accgcgtccg gcagcgcacg
      601 ccgcagctgc gccagggtca agccgtcagc agcgtcagtc ttgttgacca ccaacaactc
      661 tggcgccggt ttcccatcgt gatcggcaat cacgtcgttg aggacctgcc gcactgcgtt
      721 gatctgggcc agcggattga catccgaccc gtcgaccaca tgcaccagta ggtcggcgtc
      781 gacgacttcc tccaatgtgg agcggaacgc ctcgaccagc tgggtcggca gatgccggac
      841 gaacccgacg gtgtcggtaa gtacaaaagg ccggccgtcc gcgaattccc cacgccgcgt
      901 cgtgggatcg agggtcgcga acaacgcgtt ctcgaccagc acgcccgcac ccgtcagcgc
      961 attgagcaga ctggacttgc cggcattcgt ataaccgacg atcgcgatcg acggcacgtc
     1021 gctggccagc cgcttgctgc gctgggtgtc gcggacctgc ttcatcgccc gaatgtcgcg
     1081 gcgcagcttg gacattcgct cgcggatgcg acgacggtcg gtctcgatct tcgtctcacc
     1141 ggggccgcgc agacccacgc cgccgccgct gccgccggca cgcccacccg cctgccgcga
     1201 catcgactcg ccccagccgc gcagcctggg cagcatgtat tccatctgcg ccaaggccac
     1261 ctgcgccttg ccttcccggc tggtggcgtg ctgggcgaag atgtcgagga tcagcgcggt
     1321 gcggtcgacg accttcacct tgaccgcctt ctccagcgcg gtcagctgcg cgggactgag
     1381 ctcgccgtcg cagatcaccg tgtccgcgcc ggtggccagc accacctcgc gcagctcctg
     1441 cgccttgccg gagccgatgt acgtcgaggc atcgggcttg tcgcggcgtt gaatcaggcc
     1501 ctcgagcacc tccgagccgg cggtttcagc gagtgcggcc agctcggcca tgctcgcgtc
     1561 ggcgtcggcg gcgctgcctt cggtccacac gccgacgagc acgacgcgct ccagccgcag
     1621 ctgccggtac tcgacttcgg agatgtcgcc gagttcggtg gacagcccgg cgacccggcg
     1681 cagggcggag cggtcctcga gggcgagctc gcccgcgctg ggtgtgccgt taatggggaa
     1741 atcgggatac gtcataagcg ataacagatt tgcacggcta gatcgattcg tgcacttgaa
     1801 ttaacgctgc gcaatgcgcc accattcctc ggacaattcc ccccgggcca ccaggacgga
     1861 cggaccgcgc agataactgg tggcatcggt gaccgtaacg gtgacttcgc caccgggtat
     1921 ccgcacctgc agcgtgcccg tggtcgcgcc atcgaatgcc agcgccgcca cggcggcggc
     1981 gacagttccg gtgccacagg atcgcgtctc tccgacacct ctctcgtgta cccgcatcga
     2041 taccgctcca ccatgcggcg ccgtcagaac ctcgatgttc acgccttcgg ggaactgcgt
     2101 gcgatcgaac gaaaccggtg cggcgacgtc aagggcagcg agttcgtcga agctcagatc
     2161 ggcgtcgaca cacgccagat gcgggttacc cacatcgatg gccagacccg cgaacgggcg
     2221 gccgccgacg atcgcctcac ccgtgccgag ttggctggtc ttgcccattt cgaccgtgac
     2281 gtcggcggtg gtgtcgtccc aggcgtgcag caccacgggg cggggccccg ccagcgagcc
     2341 gacggtgaat tcgtcgcggc tctccagtcc attggcccgc aagtagtggg cgaacacccg
     2401 gaccccgtta ccgcacatct gtgcgatgga tccgtcggcg ttgcggtagt ccatgtacca
     2461 atcgcctggc tgcacgccct ctgggatccg ctcgaggccg gccacctcgg ctcgagtcac
     2521 ccgcagcacc ccgtcggcgc ccaaccctcg gcgacggtcg caaagcgcag ccaccgcggc
     2581 cggcgtcaac gacaaccggc cgtccgggtc gggcaacagt acgaagtcgt tctgcgtgcc
     2641 gtgccccttg gcgaactcca cctgctcagg atacgtgccg ccatatttgc agcgtgttct
     2701 cgacgttgtc ggcagcggcg ccgtcgagcc acgtgatgcg gtggtcgcgg cggaaccacg
     2761 accgttggcg gcggacgtag cggcgggtgc cgaagaaggt cggctcgcgg gccgcggacc
     2821 cgtcgccacc ctgatcgagg tcggcgatca cgtgggcgta gccaagggcg cgcgacgcgg
     2881 tgacgccgtc ccgcagaccg gcgcccaata acccctggac ctcgccgacg aggccgtcga
     2941 cgaacatctt gtccgtccgc tgggtcagcc ggctgtcgag aagcgctgtg tcccaatcta
     3001 atccgatgat cgcggtgttc cagcggggcg taccgatcac cggcgccgac gcagcgaagg
     3061 gttgtctggt gagttcgacg acctcaagcg ctcgcacgat gcggcggccg tcggtgggca
     3121 ggatcgagga cgcggcctgg gcgtcgaccc gcgccagttc ctggtgcaac gcgccgacgc
     3181 cgatctcggc cagccgctcc tcccatttgg cacgcacggc gggatccgtt gcgggaaaag
     3241 accattcgtc gagcagcgac tggatgtaca tcatcgaccc gccgacgatt atcgggaccg
     3301 cgccgcggcc ggcgatcgct tcgacgtcga cggtcgcggc ctgctgatag cgggcgacac
     3361 tggccgtctc ggtgacgttg aggacgtcga gctggtggtg cgctatgccg cgacgctcgg
     3421 gcacggccag cttcgcggtg ccgatgtcca tgccgcggta gagctgcatg gcgtcggcat
     3481 tcacgatctc gccgcccacg cgttcggcga cggccaccgc aagcgccgac tttccggtgc
     3541 ccgtcgggcc gatgatcgcg atgggtctca tggccgccag acgccgacgt agtagccgac
     3601 gccgtagggc gcaccgcggt acagctcttt ggccgaccga ggactccccg ccagcccggc
     3661 caatacctgg taagcgactc ggccgacgac ggtgtccggc aggcgggtca gcgcggcggc
     3721 atcgccggcg gcgagcgcgt cgtcgagcgc ggcctgcacc ggtatcgagt cggggtcgta
     3781 gccgcctggc gcgggtggcg tcagggtgtg cgcgccgtcg gcgacgacga ggacaccgat
     3841 cgggtcgacc gcctcgtcga tttcggcccg taaccggcgg ccgctggcga tcgcggcgtc
     3901 gatgtcgtgg tacgtggcgt acacccgcac ctccgcgctt gtgtcgggct tggcctggtc
     3961 gcggacccag cccgcgatca acgcgcacag cggcagttcc gtgggagttc cagcagcgtt
     4021 gggcgacagc gtgacacggc gatcgacgcc gtagcccgcg agagtgccgg cggtctcgga
     4081 atccagtaca gcgtcggcct cgccgacacc gagcgcgatc caccgcgacg gcagcgatcc
     4141 cgtagcggtg aacaccgcct cccgcaagtc ggccagttcg tcggccgcac ccgcgcccag
     4201 ttcgggaacc atgacgggtg ccgacgggat gattgcgatg gcgctcagca cgccaccaaa
     4261 gtagtgctca cgctggctgc ttggtggcac cggccgcttc cccgcgggcc agcgcgaccg
     4321 tcgacaccac catcaccgcc accgcgatga tcagggtcag ccaaccggcg tcgccgggcc
     4381 gcagcgtctc gccaagcacc acgatgccga gcgccgaccc cacgaccggc tcggcgacgg
     4441 tcatggtcgg cagcgacgcg gtcagcgagc cggcccggaa cgacgactgc tgccaggccg
     4501 tgccggcaat gccgaccaat gcccacacgt agagctcggg tgtgcgcagc agcgcccaca
     4561 gaccgtcgtc gaggcggtcc accacaccct tggtcagcac cgcgaacacg ccccatagcg
     4621 ctccggacac cagcgccagc agcaccgcgc tgacggatcc ggaccagatt cgcgcgccca
     4681 ccacgcagag cacgagggcg gggcccagca ccgcgatgac cgccgtccac gtctcgtacg
     4741 atgcccggga atggcctgcg gtggggttgc cgacggtcac gatgatcgcg accgcggcgg
     4801 ccagcagcgc ggcccacgtc cattcccagc gggtcactgg ccggtgcgac agtcgcgcgc
     4861 tgatcggcag cgcgaacaac agcga
//
LOCUS       DASRJA010000055         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3606094,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000055 DASRJA010000000
VERSION     DASRJA010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3606094"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFC01_02320"
     CDS             complement(<1..224)
                     /locus_tag="VFC01_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78513.1"
                     /translation="MSPRNASLRRLVFLAPLAATAVLGGSAIEDPSVACAEPKQWNQA
                     GYQACVDQLNKDLAAGRYTPEQYIELVQGC"
     gene            863..1186
                     /locus_tag="VFC01_02325"
                     /pseudo
     CDS             863..1186
                     /locus_tag="VFC01_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            1208..1828
                     /locus_tag="VFC01_02330"
     CDS             1208..1828
                     /locus_tag="VFC01_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78514.1"
                     /translation="MPSSEYGRETRAQILRTAMDTASVKGLSGLSIGDLAARLGMSKS
                     GLFRHFGAKEQLQLATADAALDVFERAVVAPAMAASPGIDRLRALMHSWVGYLESDVF
                     PGGCFFAAASADVDSQPGPVRDRIAGIGRAAVDAIAAEVEAAQHLGQLRLDVDARQLA
                     FEVHAYALEANWARLLLDDDHAGGRARTAIDAALARAAATTEENES"
     gene            1825..2856
                     /locus_tag="VFC01_02335"
     CDS             1825..2856
                     /locus_tag="VFC01_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN78515.1"
                     /translation="MKYVNHIDPVGPTEARGVVADVYAEARHEFGRLPEPVSMLSPHE
                     ELLAAWWATLREILIAGTAPRARKEAVAAAVAVTQRCPWCIDAHTTMLYAAGESKTAT
                     AILADTELSDTDPNAPYVAWARGTAAPGGPAPPFGPDDAVEYIGTAMIFHFVTRLVLV
                     LLDETFLPGGPRAQSLMRRAAGIAFARKVRAEHRPGLATARLAPYPLPDDLMWAAASP
                     PAATSLAALTGCLHESQHFPEASRAVVRGAITAWRGEPMPISSTWTDEHTDGLPEELR
                     SATRLALLTSLAPHQVTDADVAAARTLLATDTSFVSTLGWAAWTAARRVGSWITRPAV
                     TTSNARRMR"
     gene            2853..3206
                     /locus_tag="VFC01_02340"
     CDS             2853..3206
                     /locus_tag="VFC01_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN78516.1"
                     /translation="MSAANVIRQYHEAWTQRDMGTARSCLADDLHFQGPIDTFSSADE
                     FIATLTGFAQMLTRVDLLQEFYTDDAAALLYDCVTNSPAGTIRTAEFFGLRDGKVANI
                     RLVFDATALRQVMSG"
     gene            3321..>3915
                     /locus_tag="VFC01_02345"
     CDS             3321..>3915
                     /locus_tag="VFC01_02345"
                     /inference="COORDINATES: protein motif:HMM:NF020922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HZN78517.1"
                     /translation="MTKHGTTDKSPTLTCAFRFPAETVAFLTDLRTHNDKAWFDANRA
                     RYESAYVESATAFVEVIGPELKKLVPGIRVEPRVGGSIFRINRDIRFSADKRPYKDHL
                     DFWFWEGDRKSAVSALFLRVWPDGVVVGVGAHDFDKYQLATYRDAVVDPTAGAELATI
                     VNQLEAAGHEIGGETYKRTPRGYAADGVGERLLRHSAL"
BASE COUNT          692 a         1260 c         1252 g          711 t
ORIGIN      
        1 cagcacccct ggacgagctc tatgtattgc tccggggtgt acctgccagc tgcaaggtct
       61 ttgttgagct gatccacgca cgcttggtag ccggcctgat tccattgctt cggttcggcg
      121 cacgcgacgc tcggatcttc gatcgcactg ccgcccaaca cggccgtcgc cgctaacggc
      181 gccaaaaaga ccaggcgacg caacgacgcg ttccgaggtg acatgcgtcg acctccctca
      241 gttgaggggc ctaacaccga tccccgcacc ggacggaagg tcccgaccgc cggatgacac
      301 aaattgtccg cctgacaggg gcgggctttc ggttgtttca caggaagtcc cctgggatca
      361 ccagcggaaa gttgacatca caaccacttt cagccgcgtg catccatgcg tcctggcacg
      421 cctcggcttt gaagatccgc cgccgtcccg accagccgca tgagcaggcc gcacgggaat
      481 tcgcgactct cactcggtag accctcacga tccgaggcgc gcttgaccgg acgaccgtgg
      541 tcgcggcttc ttcctcggaa tacatgctga aatgctgacg cgttgcggcc aactcccgga
      601 acgggatgcg ggcccgtgtc cgcgggatcc gtctaggggg cgtctccgcc ggtcacgcta
      661 ggggattggg ggatcatctt cagcgccacc acgctgcgcc cagctaaacc cgcgtgcatc
      721 gtctaggcag tgtgctgcat cgtcggaagt agtcgttgac gacgcaatca ccaggtcaat
      781 tcaaacagat ctattcgttg agtcccggga gctgacccga tatcgttaag cacgaacgtt
      841 cgtgctcaac ggagggcgac cggtgcgcat atcgctgatg caggtcatca ctgaccagac
      901 gccgccgccg caagccgtcg ccagatgggc ggaggagcgc ggattcaccg ggttgtacgt
      961 gcccgagaca tcccatgtgc cgatcagccg gcagactccc tggcccggcg gggagcttcc
     1021 cgactggtac ctacgctgtt acgacccctt tgtcgcactg gctgcggccg cagcggtaac
     1081 cacgcgattg cggatcggga ctggcgcgtg cctggtcgcg gtgcacgatc caatcctgtt
     1141 ggcaaagcag atcgcttcgg tgtcggacat gtcgggtgga cgcttttcgg ggcggagatg
     1201 acgaaccatg cctagctcgg agtacggccg cgagacgcgg gcgcagatcc tgcgcacggc
     1261 aatggacacc gcgtcggtca agggactatc ggggctgtcg atcggcgatc ttgccgctcg
     1321 gctcggaatg agtaagtcgg gtctattccg gcatttcggc gccaaggaac aactgcagtt
     1381 ggccaccgcg gatgccgcgc tcgacgtttt cgagcgggcg gtcgtggcgc cggcgatggc
     1441 ggcctcgccc ggtatcgatc ggctgagggc cctgatgcac agctgggtcg ggtatctgga
     1501 aagcgatgtg tttcccggag gctgcttctt cgcggcggca tccgcggacg tcgactctca
     1561 acccgggccg gtccgcgacc gaatcgccgg tatcgggcgc gcagcggtcg atgccattgc
     1621 ggcggaagtc gaagccgcgc aacaccttgg ccagcttcga ttggatgtcg acgccaggca
     1681 gctcgcgttc gaggtgcatg cctacgcact cgaggccaat tgggcccgac tgctgttgga
     1741 cgacgatcat gccggcggcc gcgcccgaac cgcgatcgat gcggcacttg ccagggcggc
     1801 ggccactacg gaagagaacg aatcatgaag tacgtcaacc atatcgatcc tgtcggaccg
     1861 actgaagcgc gcggcgtcgt cgccgatgtt tacgccgagg cccgccacga gttcggccgg
     1921 ctgcctgagc cggtcagcat gctctcgccc cacgaggagt tgctggccgc ctggtgggcg
     1981 accctacgag aaattctgat cgccggaacg gcgcctcgcg cgcgcaaaga agcggtcgct
     2041 gctgcggttg cggtgaccca gcgctgcccg tggtgtattg acgcccacac gacaatgttg
     2101 tatgcggcgg gcgaaagcaa aactgccacc gccattctgg ccgatacgga gctatccgac
     2161 accgatccga acgcacccta cgtcgcgtgg gcccgcggca ccgcggcacc gggcggtcct
     2221 gccccgccgt tcggacccga cgatgcggtc gaatacatcg gcaccgcaat gattttccac
     2281 ttcgtgacgc ggttggtgct ggtgctgctc gacgaaactt ttctccccgg cggtccccgc
     2341 gcccaatctc tgatgcgccg cgccgccgga atcgcattcg ccaggaaggt gcgtgcggaa
     2401 catcgaccgg ggttggcgac cgcccggctg gcgccatacc cacttccgga cgatcttatg
     2461 tgggccgcgg cttcgccccc ggcggcgacc tcgctggctg cactaaccgg ctgtcttcac
     2521 gagtcgcaac attttcccga ggcaagccgc gctgtcgtcc gcggcgcgat caccgcatgg
     2581 cgaggggagc cgatgccgat cagcagcacc tggaccgacg aacacaccga cggtctgccc
     2641 gaggaactga gatcggcgac ccgccttgcg ttattgacaa gccttgcccc gcatcaggta
     2701 acggatgcgg atgtggcggc cgccaggacg ctgctggcca cggacacgtc cttcgtctcc
     2761 acgctgggat gggcagcgtg gactgcggcc cgtcgcgtcg gatcctggat tacccggccg
     2821 gccgtcacca caagtaatgc caggaggatg cgatgagcgc agcgaatgta attcggcagt
     2881 atcacgaggc gtggactcag cgcgacatgg gcacggcgcg ttcctgcttg gccgacgatc
     2941 tgcattttca aggtccgatc gacacgttct cgagcgccga cgaattcatc gccactctta
     3001 ccggctttgc gcagatgctc actcgtgtag atctgctcca ggagttctat acagacgacg
     3061 ccgccgcgtt gttgtacgac tgcgtcacca attcgcctgc cgggaccatc aggacggctg
     3121 agttcttcgg gctgcgggac gggaaggtcg ccaacattcg cctcgtgttc gatgccaccg
     3181 cgctgcggca ggtcatgagc ggctaactgc ggcacgcgaa tagttcggtg gcgttgtcgg
     3241 cgggtttgcc tccgatatgt cggaatgctg ggtgttcgtt cgtcggatca gaaacgcagt
     3301 ctcaccgcat gggagtagtc atgacgaaac acggcacgac agacaagtcc ccgacgctga
     3361 catgcgcgtt ccggttcccc gcagagacgg tggcgttcct gaccgatctg cgtacccaca
     3421 acgacaaagc gtggttcgac gcgaatcgtg cgcgctatga gtcggcgtac gtggaatcgg
     3481 ccacggcgtt cgtcgaggtg ataggacccg agttgaagaa gttagtgccc ggcatccgtg
     3541 tcgagccgcg ggtgggcggc tcgatcttcc ggatcaaccg cgacatccgg ttcagtgccg
     3601 acaagcgtcc atacaaggat cacctcgact tctggttttg ggagggtgac cgcaaatcgg
     3661 cggtgtccgc cctcttcctg cgcgtctggc ccgatggcgt tgtcgtcggt gtcggcgcac
     3721 acgacttcga caaataccag ctcgccacct accgcgacgc cgtcgtcgac cccaccgcgg
     3781 gcgccgaatt ggcgacgatc gtgaaccagc ttgaggcggc tggccacgag atcggtggtg
     3841 agacctacaa gcgcacacca cgtggctacg cggcagacgg cgtcggcgag cggctactac
     3901 gccactccgc gctgt
//
LOCUS       DASRJA010000056        14676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2198842,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000056 DASRJA010000000
VERSION     DASRJA010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14676
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198842"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFC01_02350"
     CDS             <1..392
                     /locus_tag="VFC01_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZN78518.1"
                     /translation="ALNNWLAVLVEGMVETLTLSEALGIDPRLLLEAIGDGPMASDYA
                     LAKGAAMINAEFAPGFPLRHATKDAELALSAAHRHGVELPLTSALLPRWHQALAGDHG
                     GDDVASAVTAAATTASPPGSLLSIAAS"
     gene            389..1015
                     /locus_tag="VFC01_02355"
     CDS             389..1015
                     /locus_tag="VFC01_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HZN78519.1"
                     /translation="MTLSSRTEILSETTHPATDPHTSHITNRLNWLRAGVLGANDGIV
                     STAGLVVGVAAATTNSSVIFTAGLAGLVAGGVSMALGEYVSVSTQRDTQRALLQKERQ
                     EPDEMPEAELGIDPGDLSNPWQAALSSAVAFTLGAVVPLFAILLPPVGARVPVAFLSV
                     LIALVLTGTVSAMLGGARKRRAVVRVVLGGVLAMLVTYGIGQMVGTTI"
     gene            1079..2620
                     /locus_tag="VFC01_02360"
     CDS             1079..2620
                     /locus_tag="VFC01_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZN78520.1"
                     /translation="MSARLVGLLVRPTAPPLAVGIAVAASLIVAETLLVYLLEKIAPG
                     MTFGVIFLLGVLVVSASWGFGLAVTTTLASALVYAFVHLGADGSIFPITGQDWVTIII
                     FLPIALLANVLAGQARLLAAEADQRRREAEASRDELRVLADQQAALRRIATLVARAVE
                     PSEVFSAVTEELARCLGVTHATLVRFEADGAAVLLASHDDRGSQKKIPVGKRFSFDGA
                     SVPAVVFRTGRAARRDSHENAPSPEIQYIHELGLRSGVGVPIVVAGRLWGAANVGSSQ
                     PEPLPADTEARVGDFADLVATAIANADARSELTASRARIVAASDAARRRFERDLHDGA
                     QQRLVSLGLQLRTAEASLPPECQALRAQISDIVTGLGGVSEDLQEISRGIHPAILSRG
                     GLGPALRTLARRSAVPVELRLGIDRRLPESAEVAAYYVVAEALTNAAKHAQASEVRVT
                     VESDGANLRLSISDDGVGGADSHKGSGLIGLIDRVEALGGQLRISSLVGSGTSLLATI
                     PFDGS"
     gene            2785..3066
                     /locus_tag="VFC01_02365"
     CDS             2785..3066
                     /locus_tag="VFC01_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78521.1"
                     /translation="MRMKLTHITPLLAAGAAAAAIAAAPTAAATPAAAELAAPSAAVT
                     SQVAPAAFHGGGGFHGGGFHGGGFHGGGFHGNHYGWGWHPWGWPWGRPW"
     gene            3277..3567
                     /locus_tag="VFC01_02370"
     CDS             3277..3567
                     /locus_tag="VFC01_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78522.1"
                     /translation="MRIKLSYMTPLLGATAAAVAIVVAPMAAAAPAGPGAIQESCGPS
                     GSGTVCQSPGNAEINDSPPPVNFYPYGGDAFLLGGGGFGGGGFHGGGGGGHR"
     gene            3752..3991
                     /locus_tag="VFC01_02375"
     CDS             3752..3991
                     /locus_tag="VFC01_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78523.1"
                     /translation="MQLGLHYIAPLLATGAAAVAIAAAPLAAAAPSAPGDNTQTQQSC
                     ANLGGTQTECQAPGNVQVNDAPPQVDYFPYAGGGT"
     gene            4452..5588
                     /locus_tag="VFC01_02380"
     CDS             4452..5588
                     /locus_tag="VFC01_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HZN78524.1"
                     /translation="MKQFTDQPIGCRTKVEQMWGKRRDMTVRDRSPFNAEPPASVLAS
                     AEITALDAFYSRNHGPFPDIARDQWRLEVDGLIDTPLTLTYDQLISRFTPVSVVATLA
                     CAGNRRAELLNVRPIPGKDPWAHGAISTAEWRGARLADVLQAAGVHHDDSLHVAFSAP
                     DVAQEALPVQAYGSSIPLSKAMSREVLLAWEMNSEPLPRVHGGPVRVVVPGYIGARSV
                     KWVNAVTVQSGPSQNYFQASDYRILPPDADPDTAAPGDGISLSSLPLNCDILVPGDDE
                     QVPAGPLRISGWALAGDGRGIGRVDVSLDGGGTWRQADLQPATSQWAWRLWSLTVDAR
                     PGPISVTARAWDETGVTQPESPAHLWNPRGYGNNAWAHVKAFAK"
     gene            6124..8625
                     /locus_tag="VFC01_02385"
     CDS             6124..8625
                     /locus_tag="VFC01_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZN78525.1"
                     /translation="MTQKQSRQPGSAWLPGNIFAGRGSDAGMGPRNMRLRLLALVSRP
                     KAPSLVLGIAVAVAFIAVETLVVCSLNVVTDSTGRFGTLFLLGVLVVSMVWGFGLAAT
                     MSIASAIAFAYFRDWPTAHFAPFEPQNWIPIGVFLVFALVANALAGLARVGERFFDLS
                     PDLLCITGPERVIRINPAFSQILGYSLDDLASRPYLDLVVPEDRDHVGSFLEQLPGSA
                     DPVRFENRVICKEGSPRWIEWSVVWHRGLFYAVGRDVTERRREQDELQAARAVAEASR
                     DRISQLAEQQAALRRVATLVAHGTSPSKVFAAVADELAGVLHVVNAGLLRYEPDGTGL
                     VVAVRYEPGITQMPNIGERIPLAGDDVGARVLHTGRPARIDDHAATPGAEAERIRESG
                     VGSIVGVPIVVDGQLWGAAIVGSTGPHPLPADTEARIADFADLLATAIANAATRAELQ
                     ASRDELSVLADQQAGLRRVATLVARGVSPSEVFCMVADEMAGRLHAKNASVNRFEGDE
                     VVVLALSHLDEGMQNKPVVGERHTLEGDNIATRVIQTGRAARLDPPEMQDAPGSIAAR
                     LREMGLYSTVAVPIVVDGQVWGMAAVGSSTPDPLPPETEARVGDFADLVATAIANAAT
                     RAELVASRARIVAAADDARRRLERDLHDGAQQRLVSLGLQTRRAEASVPPQLDGLKSQ
                     LSRVVSGLTDVSLDLQEISRGIHPAILSKGGLGPALKTLARRCTVPVSLDVAIDRRLP
                     DSVEVGAYYIVAEALTNTAKHSRASQVEVCAESKDDNLYLSIRDDGIGGADVAKGSGL
                     IGLKDRAAALGGRMSIDSPVGSGTSLQVTIPLDNS"
     gene            9409..10089
                     /locus_tag="VFC01_02390"
     CDS             9409..10089
                     /locus_tag="VFC01_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN78526.1"
                     /translation="MASLAATVPLHFAGDRFDAEESAPVKSHSAPTARPVLLVHGLGG
                     TKSSWSFLARTLTARGLTVDAITYSPFGTSVEQVADRLAVKVERLLSQTGADKVHLVG
                     HSLGGVVIAQAITSGRLTGLVDTVVTLAAPFGGSPWANLLPFGAIVSALRQGSPLLQR
                     IASVPAPDGMRWVAFTAEHDTIVPGRRSVPPHVNAETVTLDGVGHLGMLVSPQAVGRI
                     VAALSAHE"
     gene            complement(10110..10316)
                     /locus_tag="VFC01_02395"
     CDS             complement(10110..10316)
                     /locus_tag="VFC01_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78527.1"
                     /translation="MITRLKGFARFWYDFFVGDDWTVAVGVVLALAATYALSVAGVNA
                     WWITPAAVLILLAASVRRAARSRN"
     gene            complement(10313..11050)
                     /locus_tag="VFC01_02400"
     CDS             complement(10313..11050)
                     /locus_tag="VFC01_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78528.1"
                     /translation="MNSAALFLTVFLACAVEAVEATTVVLAMGTARDWRSALSGVAAG
                     LLLLVAVVAVLGPAISAVPLDALRLLVGGVLLIFGLQWLRKAVLRASGHKALHNEDAV
                     FRQQLAAAQSASAGGRGLVPDWYGFTVVFKSVVLEGLEVVFIAVTFGANAHDVPVAAV
                     AALIAILVVAGVGAVVRAPLSRVPENTMKFAVGVMLCAFGTFWGAEGAGVSWPGADLA
                     IVVLVLLYGCVAVMLAAALRDRSKASS"
     gene            11510..11665
                     /locus_tag="VFC01_02405"
                     /pseudo
     CDS             11510..11665
                     /locus_tag="VFC01_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263883.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
     gene            complement(11676..12314)
                     /locus_tag="VFC01_02410"
     CDS             complement(11676..12314)
                     /locus_tag="VFC01_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZN78529.1"
                     /translation="MTEIIAGVTIPDSVVVKAATELVLDAEDDLLFHHSRRVYLWGML
                     KSRRRRHSPDPELSYVGAMFHDLGLTPKYRTAHQRFEVDGADIAEQFLLDQGYSPAEA
                     RMVWLSIALHTTPGIPEHLAPEIHLVTLGVETDVLGQQIYEISDEQLAAVIAAHPRPD
                     FKNRIQRAFYDGMRDRPETTFGTMNDDVIAHFDADFRRKDFVSIIAQSAWPE"
     gene            complement(12406..13887)
                     /locus_tag="VFC01_02415"
     CDS             complement(12406..13887)
                     /locus_tag="VFC01_02415"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HZN78530.1"
                     /translation="MTDMQPTPPTTTNAGIPVESDEHSLTIGPDGPILLHDHYLIEQL
                     AQFNRERVPERQPHAKGSGAFGRFEVTHDVSAFTKAAVFQPGTTTETVIRFSTVAGER
                     GSPDTWRDPRGFALKFYTTEGNYDIVGNDTPVFFLRDPLKFPSFIRSQKRRADSNLRD
                     HDMQWDFWTLSPESAHQVTWLMGDRGIPRSWRYMNLYSGHTFMWVNAAGERFWVRYHF
                     ITDQGIQNLTQAEADALVSVDTDYHTRDLFEAINHGDYPSWTLKMQIMPFEDAKTYRF
                     NPFDLTKVWPHADYPLVEVGRLTLDRNPTDNHSQIEQGAWSPSNLVPGIGASPDKMLL
                     GRMFAYADAQRYRIGANYNQLPVNAPKCPVHSYNTAGQMRYQLATDPVYAPNSKGGPR
                     ADTERYGEPAGWHTDGEMVRTAYTLRSDDDDWGQAGTLVRDVMDDAERERLVDNIVGH
                     LLDGVTEPVLQRAFEYFRNVDMDLGERVEKGVRAGALVGGADR"
     gene            complement(14031..>14676)
                     /locus_tag="VFC01_02420"
     CDS             complement(14031..>14676)
                     /locus_tag="VFC01_02420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN78531.1"
                     /translation="SRGSHISRPEPLRQVTISRVPPAAALNPMAARSLVGLAGLPTIL
                     ATGPFDDQADADQLAAAFATVRSRCRAQLVLLGGGEYRATVLRRSFAQGAGSSVHVIR
                     DLTEDRWPYLIAAADVVVPENATGYTTLLDVLGAGRPVVAQADPTIVRLVVPASAGLI
                     YRRGDVSGMAGALLRLLTTPALRHGMGCRARTVARRYHLQRMALQRIDQRNEYA"
BASE COUNT         2627 a         4661 c         4717 g         2671 t
ORIGIN      
        1 tcgcgctcaa caactggttg gccgtgctag tggaagggat ggtcgaaacg ctcacgctca
       61 gcgaagcact cggcatcgac ccacggctcc tgcttgaggc tattggcgac gggccgatgg
      121 cgtcggacta cgcattggca aaaggcgccg cgatgatcaa cgccgagttc gcgcccggct
      181 tcccgttgcg acacgcgacc aaggacgccg aactcgcact gagcgccgct caccgtcacg
      241 gcgttgagct cccgctgacc agtgcgctcc tgccgcgctg gcaccaggcc ctcgcaggcg
      301 atcacggcgg cgacgacgtc gcctctgccg tcaccgcggc agccacaacc gcgtcgccac
      361 ccggcagtct cctgtcgatc gctgcgtcat gacgctgtct agtcgtactg aaatactcag
      421 tgaaacaacg catcccgcga ctgacccgca tacgtcgcac atcaccaacc gactgaactg
      481 gctacgcgca ggggtgctcg gggcgaacga cggaatcgtc tccaccgcag gcctcgtggt
      541 gggcgtagcc gcggccacca caaacagttc ggtgatcttc actgctggat tggcaggctt
      601 ggtcgctggc ggcgtctcga tggcgctcgg cgagtatgtg tcggtgagca cacaacgcga
      661 cacgcaacgg gcgcttctgc aaaaggagcg gcaagaacca gacgagatgc ccgaggccga
      721 actcggcatc gacccgggcg atctgtccaa cccctggcag gcagcgctgt cgtcggcggt
      781 cgcgttcacg ctgggcgccg ttgttccgct gtttgcgatt ctgcttccgc ccgtcggcgc
      841 ccgtgtcccc gtggcctttc tgtcggtgtt gatcgctctc gtgttgaccg ggaccgtgag
      901 tgcgatgctg ggcggtgccc gcaagaggcg cgccgtcgtg cgtgtagtgc tcggcggagt
      961 tctcgcgatg cttgtgacct acggcatcgg gcagatggtc ggaacgacga tttgagagaa
     1021 gcctgtgggc gaggtgcgcg tggcagacag ttctgatgcg gggacggcgc cgccattgat
     1081 gagcgctcga ctggtcggac tgttggtgcg ccccacagcc ccaccgctcg ccgtcggcat
     1141 cgcagttgct gcgtcgctca tcgtcgcaga gacactgctg gtgtatctgc tggagaaaat
     1201 cgccccgggc atgacctttg gtgtcatctt cctgctcgga gttctcgtcg tatccgccag
     1261 ctgggggttc gggctcgcgg tgacgacgac gttggccagc gcgcttgtct acgccttcgt
     1321 ccatctcggg gccgacggca gcatcttccc tatcaccggc caagactggg tgaccatcat
     1381 catcttcctg cccatcgcgc tattggcgaa tgtgcttgcg ggccaggcgc gattgctcgc
     1441 cgcggaagca gatcagcgcc gccgggaagc agaggcaagc cgagatgagc tccgtgtgct
     1501 cgctgaccag caggccgcgc tgcgccgcat tgccacgctg gtggcgcgtg ccgtcgaacc
     1561 ctccgaggtg ttctcagccg ttacagagga attggcgcgg tgccttggag ttacccacgc
     1621 aactctggtg cgcttcgagg ccgacggcgc ggcagtactg ctcgccagcc acgacgaccg
     1681 cgggtcgcag aagaagattc cggtgggtaa gcgtttttcg ttcgatggcg caagcgttcc
     1741 ggcggtggta tttcgcaccg gccgcgccgc ccggcgagac agtcacgaga atgcgccctc
     1801 cccagaaatc cagtacatcc atgagctcgg tctgcgctca ggcgtcggtg tgccgatcgt
     1861 tgtggccggc cggctatggg gcgcggccaa tgtcggatcg tcgcaacctg agccgctacc
     1921 tgccgatacc gaggcacgcg tcggggactt cgcggacctg gtcgcgacag cgatcgccaa
     1981 cgccgacgcc cgctccgagc tcacggcttc tcgggccagg atcgtcgcgg cctctgacgc
     2041 cgcccggcgc cgattcgaac gcgacctgca cgacggtgca caacagcggc tcgtgtcgct
     2101 ggggctccag ctgcgcacgg ctgaagcgtc gttgccgccc gaatgccagg cgttgagggc
     2161 acagatttct gacatcgtta ccggcctggg gggtgtatcg gaagatctcc aagagatatc
     2221 gcgcggtatt catccggcaa tcttgtcgag aggtggactg ggcccagcgc tgagaactct
     2281 ggctcgtcgc tccgccgttc cggtggagct gcgcctcggc attgaccgac ggttgcccga
     2341 gtcggccgaa gtggccgcct attacgttgt cgcggaagcg ctcacgaatg cagcgaagca
     2401 cgcgcaagcg tccgaggtgc gggtaactgt cgaatccgat ggcgcgaact tgcgactctc
     2461 gatttccgac gacggagttg gcggcgccga ctcgcacaag gggtccggac tgatcgggtt
     2521 gatcgaccga gtcgaggcgc tcgggggaca gctgcggatt tccagccttg tcgggagcgg
     2581 cacatcactt ctggcgacga tcccgttcga cggatcttga ccgtcatacg ttgccgccgc
     2641 ggctgattcc gggtagcagg gacgcatcgt tgggtgcaag cgctgacgac acgactctgc
     2701 ccgagcgcga ctctggaagt gcaagagaca gattcgccgg tttaccgatc acagctaggc
     2761 cggctatcac gagagagtgc aactatgcga atgaaactga cccacatcac gccgctgttg
     2821 gctgcgggcg ctgctgcggc ggcgatcgcc gcggcaccaa ccgccgcggc cacgccggct
     2881 gcggcagaat tggccgcccc atcggcagcg gtgacgagcc aggtcgcccc cgccgccttc
     2941 catggcggcg gtggctttca cggcggtggc ttccacggcg gtggcttcca cggtggcggc
     3001 ttccacggca atcactacgg ctggggctgg cacccttggg gatggccgtg gggccggccc
     3061 tggtagcgat caatgacgat ggcggaagcg gtgtttgaca tcgcttccgt cgtcgttgcg
     3121 tacccattcg ttgcacttct gcggtgcacg ctgacaggta cacggagatt cccggtggcg
     3181 gccccacaga tgggtggcag cagtgacgac atggcctcgt tcagagacaa ctctggaaca
     3241 cggacagaca caaacgacta tcaaaggagt gccaccatgc gaatcaaact gagttacatg
     3301 accccattgt tgggtgcgac tgctgccgct gttgccatcg tcgtcgcacc gatggcggcc
     3361 gccgccccgg ccgggccggg tgcaatccag gagtcctgcg gcccaagcgg ttcgggaacc
     3421 gtgtgtcaat cgccgggcaa cgccgagatc aacgactcgc ctccgcctgt caacttttat
     3481 ccctacggcg gcgatgcctt tctgctcggc ggaggcgggt tcggcggcgg cggattccac
     3541 ggcggtggtg gcggcggaca ccggtagcaa tcaatgacct tgcgggggcg ccggtgtggc
     3601 atttgttggc acgcgggcgc ccgcggcatt ggccgctcag aacattcctg cttgcgggag
     3661 gattgattcg tgccagcagt gacgacaggc cctaatccgc acacaattct ggaactcata
     3721 cacatcatct agccatcgaa ggagagccac catgcaactc ggattgcact acatcgcacc
     3781 gcttttagcg acgggagctg ctgcggtagc catcgccgct gcccctttgg ccgccgccgc
     3841 gccgtcggcg ccgggtgata acactcagac tcaacagtcc tgcgctaact tggggggcac
     3901 gcaaaccgaa tgccaggcgc ccggcaatgt gcaggtcaac gacgcgcctc cgcaggtcga
     3961 ttacttcccg tacgcaggcg gcggcaccta aacgtctctc agtcaaccgg ctgctacgac
     4021 agaaaccctt tgttgcccag aggattacac gctgtttgtg cacgaccaaa ctcctatgcg
     4081 gcgcagccgt cttggtgtcg tgtcgtcgtc gtcaactgcc gatggatacc tcggccttgt
     4141 gaaccggtca aatttttctg tagctatgcg gtgctgcccg ggctgctggc ggcgtcactg
     4201 tagtccaccc gacagacgcg aggaagagat aacgagcaca acgcagctcg gcgagcgcgg
     4261 gccggcgtcc gccgcgttgg cttccggtct gcggccacgt tgcagctggc aggtgggcgg
     4321 gaagtaccgc tagcgggcgg cgtttctggg cgtcagctgc gacgacatgg gccgcgcgca
     4381 agcgcgacga taaggacggt cgagacatcc caccaaagaa gtggtcacca caatgcgagg
     4441 agacagctgc gatgaagcag ttcaccgacc aaccaatcgg atgccgcacc aaggtggaac
     4501 aaatgtgggg aaagcgccgc gacatgaccg tgcgcgaccg ctcgccgttc aacgcagagc
     4561 caccagcatc ggtgttggcc agcgccgaga tcacggcgct ggacgcgttt tacagccgca
     4621 atcatggtcc gttccccgac attgcacgcg accagtggcg cctcgaggtc gatggcctga
     4681 tcgacacgcc actcacgctc acctacgacc aactgatctc gcggttcacg ccggtttcag
     4741 tggtcgcgac gctggcatgt gccgggaacc ggcgcgccga actactgaac gtgcgaccga
     4801 taccgggcaa ggacccctgg gcgcacgggg cgatttcgac cgccgagtgg cgtggagcga
     4861 ggctggcaga tgttctccag gctgccgggg tgcatcatga tgacagtttg cacgtggcgt
     4921 tcagtgcccc cgatgtggcg caggaggccc ttcccgttca ggcgtatggc agctcaatcc
     4981 cgttgtccaa ggccatgtcg cgggaagtgc tgctggcctg ggagatgaat tcagagccgc
     5041 ttccacgagt gcacggtggc ccggtgcgcg tggtggtgcc gggatatatc ggggctcgca
     5101 gcgtcaagtg ggtcaacgcc gtcaccgtgc aatcagggcc gtcgcaaaat tacttccagg
     5161 ccagtgacta tcgcatcctt ccgcccgacg ccgaccccga caccgcagcg ccaggcgacg
     5221 gcatttcgtt gtcgtcgctt cctctcaact gcgacatcct cgttcctggc gacgacgagc
     5281 aggtccctgc cggcccgctg aggatcagcg gatgggcact ggctggcgat ggacgcggga
     5341 tcggccgagt cgacgtttcc ctcgacggag gtggcacgtg gcgacaagcc gacctgcaac
     5401 ccgcaaccag ccagtgggca tggcggctgt ggtccctgac ggtcgacgct cgacccgggc
     5461 caatcagtgt gacagcccgc gcttgggatg aaactggcgt cactcaaccg gaatcgccgg
     5521 cacacctctg gaatccacga gggtatggca ataacgcctg ggcccacgtg aaagcgtttg
     5581 cgaaatagcg gattcggcat gaggtcgggg ttgtgcgcat gtggcctcaa atagcgggac
     5641 gtcgcttccg gtcacgatcc cattgcgcta tgtcggcggt cggcgctacg gggtgatcat
     5701 gccgaccaac atcctctcgg ttgaagggtg aatcaaagcg cgcgattcaa cgcgcaaacg
     5761 tcgtctgcag ctccgacgtt catctcacag atccttgtcg cgcatgtaag cccacaattc
     5821 gcaccgccgc tgagcccact ccacagtcca tccagtaaat gccgcgcttg cgagcgtgat
     5881 tatccatgtc atcgtgtggc cccaatcact agcggttgtg gacaatagtc gcgcgctagc
     5941 aaacgaatgt cgtcacagct gacatccaga taatgtcccc gccacctggg atctgtgcct
     6001 tccagctagc gggtatgagt tacgctccgc tctaccgcac aggcatcaca ccgcgcggtc
     6061 tgcgcggcta tgctgagttt gcgcgtctgg ttgaaggccg ccgaagagcg atgtgagttt
     6121 ctggtgacgc agaagcagtc acggcaacct ggctcggcgt ggctgccggg caacattttc
     6181 gcgggcagag gctccgacgc aggaatgggc ccgcgcaaca tgcgcttgcg tctactcgcg
     6241 ctggtgagcc gccccaaggc tccgtcgctt gtgctgggta tcgcggtcgc ggtggccttc
     6301 attgcggtcg agaccctcgt cgtgtgttcg ctcaatgtgg ttactgattc gaccggccgc
     6361 ttcggcacgc tcttcctgct gggcgttctg gtggtttcga tggtgtgggg attcggcttg
     6421 gcggcgacga tgtcgattgc cagcgccatc gccttcgcct atttccgcga ttggccgacc
     6481 gcccacttcg ccccgttcga accccagaac tggattccga ttggtgtctt ccttgtcttc
     6541 gcgttggtgg cgaacgcact tgccggcctg gctcgggtag gcgagagatt cttcgacctg
     6601 tctcccgact tgttgtgcat cacaggtccg gaaagggtca tccggatcaa cccggcgttc
     6661 agtcagatac tcggctattc actcgacgac ttggcctcgc gcccttatct cgatttggtc
     6721 gttcccgaag atcgagatca cgtgggttcc tttcttgaac agctgccagg cagtgctgat
     6781 ccggtgcgct tcgagaatcg ggtgatctgc aaggagggtt cgccgcgctg gattgagtgg
     6841 agcgtcgtct ggcatcgcgg cctgttctac gccgttggcc gcgacgtaac cgaacgccgc
     6901 cgggagcaag acgaattgca ggcggcgcgg gccgtggccg aggcgagccg cgacaggata
     6961 agccaactcg ccgaacaaca agcggcgctg cggcgggtgg caacccttgt cgcacacggg
     7021 acgagcccct ccaaggtctt cgcggccgtg gccgacgaat tggccggcgt tttgcacgtg
     7081 gtgaacgcgg ggctccttcg atatgagccc gacggcaccg gactcgtggt tgccgttcgg
     7141 tacgagccgg gtataacgca gatgccgaac atcggggagc gtattccgtt ggcgggcgac
     7201 gatgtcgggg ctcgggtgtt gcacactggg cgccccgcac ggatcgacga ccacgcagcc
     7261 accccaggtg cggaagccga acgcatccgt gagtcgggcg tcggttcgat cgtcggagtt
     7321 ccgatcgttg tcgatggcca gttatggggc gcggcgatcg tcggctcgac cggaccccac
     7381 ccgttgccgg cggacaccga ggcacgaatc gccgacttcg ccgatctgct cgccaccgcg
     7441 atcgccaacg ccgccacccg ggccgaactg caggcgagtc gtgatgagct aagtgtgctc
     7501 gcggaccagc aggccggctt gcggagggtc gccaccctgg tggcacgcgg ggtcagcccg
     7561 tcggaggtgt tctgcatggt ggccgacgag atggccgggc gcctgcatgc gaagaacgca
     7621 tcggtgaacc gcttcgaagg cgacgaggtg gtcgtcctcg ccctgtctca cctcgacgag
     7681 gggatgcaga acaagcccgt cgtcggcgag cgtcacacgc tggaaggcga caacatcgcg
     7741 acgagggtga ttcaaaccgg ccgggccgcg cgactggacc ccccggaaat gcaagacgct
     7801 ccggggtcta tcgcagcacg cctacgcgag atgggcctct attccacggt ggcggttccg
     7861 atcgtcgtcg atggacaggt atggggaatg gcggccgtcg gctcgtcgac acctgatccg
     7921 ctgccaccgg aaaccgaggc acgggttggc gacttcgccg accttgtcgc gaccgcgatc
     7981 gccaacgccg ccacccgcgc cgaactcgtc gcctcacgcg cccgcatcgt cgcggcagcc
     8041 gacgacgccc gccgccgcct ggaacgcgac ttgcacgatg gcgcccaaca acggctggtt
     8101 tcgctcggac tccaaacgcg gcgagcagag gcgtccgtgc cgccgcaact cgatggcctc
     8161 aaaagccaac tatctcgcgt tgtttcgggt ttgacagacg tctcattaga tcttcaggag
     8221 atctcgcgcg gaatacatcc cgcgatcttg tccaagggcg gacttggtcc ggcgctcaag
     8281 acactggccc gccgctgtac cgtccccgtc agcctcgacg tcgcgatcga ccgacgcttg
     8341 cctgactcgg tcgaagtcgg cgcttactac atcgtggccg aggcgttgac caacacggcc
     8401 aagcattccc gcgcctccca ggtcgaggtg tgcgcagaat ccaaggatga caacctctat
     8461 ctgtcgattc gcgacgacgg aatcgggggc gccgacgtcg ccaaggggtc aggactgatc
     8521 gggctcaaag accgagcggc tgccctcggc ggacggatgt cgattgacag cccagtcggg
     8581 agtgggacgt cgcttcaagt gacgatccca ctcgacaatt cctaaggctc gcatttccac
     8641 agtcgcgaca gaagggccga tccgcgccgg gggacacata cggcacctcg ggatcggctc
     8701 ggacggcctg ctatgactag cgttgcgcgc caacacgttg tttcgtcacc gctgcgttgc
     8761 cgctagcagc aactgtcgcc tttccgtggc acacctacct tcggccgttg tagcatcagc
     8821 ccaacgttgt tgcaggatag tgaattccgt ctcgggcgcc accgagcacg tattggccgc
     8881 agcatcgatg gatgcaaagt gtcagtcggg ccaggacggg ctgacgatga cgaacctgtg
     8941 gaccgccgaa tccctttcgg gaagaagcct ctagagacat cgtggtggtc gacgatcctg
     9001 tgactcgatt cacgttggcg cccagcgcgt gagtgattcg gcacgagccc cgctcgaaag
     9061 ccgggcgggc gccggggaac ccgaggaatt catttcctga ccaaccgcac cgactctgcg
     9121 gcctgtcggt tcctggtagc gggtagtacg acccacccat tggccgcaag cactgtttgc
     9181 ggctgaccgt gatgactctc gatccatcaa gcgcgattct caaagtccaa cggtcgcaca
     9241 aaggggtgag acatgtcgca agccgtacac aacaggcatc agccggcacg ggtcgctgtg
     9301 cgaagccaac gacacgtcgg gtggccgctc ggcgcaatcg gaggcgctcg cacgctgttt
     9361 cggatcgtcg gacgcgccgc gctagtgcag gcgtacgaag tcgcgctgat ggcttccctg
     9421 gcagcgacag tgccgttgca cttcgccggc gaccgctttg acgcggaaga aagtgcgccg
     9481 gtcaaatcgc actccgcgcc cacggcgcgg cctgtgctac tcgtgcatgg tttggggggc
     9541 acgaagtcaa gctggtcttt cctcgcgcga accctcactg cccgaggtct gaccgttgac
     9601 gcgatcacgt actccccatt tggaacctcg gtggagcagg ttgccgaccg gctcgccgtc
     9661 aaggttgagc ggctgttgtc tcagacaggg gcggacaagg tccatctggt cggacacagc
     9721 ctgggcggtg tcgtcatcgc gcaggccatc acaagcggtc gcctgaccgg gctggtcgac
     9781 accgtcgtca ccctcgcagc acccttcggg ggatcgccgt gggccaacct actgccattc
     9841 ggagcgattg tctcggcgct gcggcaaggt tcgcctctgt tgcaacgcat cgcgtccgtt
     9901 cctgcaccgg acggaatgcg ctgggtggca ttcaccgcgg aacacgacac gatcgtgccc
     9961 ggccgtcggt cggtgcctcc ccacgtcaac gcagagacag tgacgctaga cggcgtcggc
    10021 cacctcggga tgcttgtgag cccgcaggct gtcggccgca tagtcgcagc gttgtcagca
    10081 cacgagtagt tgcgatcccc cccgttgcct taattgcggg agcgggccgc cctgcggacg
    10141 ctggcggcga gcagtatcag caccgccgcc ggggtgatcc accaggcgtt caccccggcc
    10201 acgctgagcg cgtatgtggc ggcgagggcg agtacgacgc cgacggcgac agtccaatcg
    10261 tcaccgacga agaagtcgta ccagaatcgg gcgaagccct tcaaacgggt gatcacgacg
    10321 acgccttcga tcgatcgcga agagccgcgg ccagcattac ggcgacgcag ccgtacagca
    10381 gcacaagcac tacaatcgcg aggtcggctc ccggccagct gaccccggcg ccttcggcgc
    10441 cccagaaggt gccgaacgcg cacaacatga cgccgaccgc gaacttcatc gtgttctccg
    10501 ggacacgaga caggggcgcg cgtacgacgg cgcccacccc tgcgacgacc agaatggcga
    10561 tcaatgccgc aacggctgcg acgggtacgt cgtgggcgtt ggcgccgaac gtcaccgcga
    10621 tgaacaccac ctccaggccc tcgaggacca cacttttgaa caccaccgtg aacccgtacc
    10681 agtccggcac gaggccgcgt ccccccgccg atgcggattg cgctgccgca agctgctgac
    10741 gaaacacggc gtcttcgttg tggagcgcct tgtgaccgct cgcgcgcagc acagcctttc
    10801 gaagccactg cagcccgaag atcagtagca cgccgccaac caggaggcgc aacgcgtcca
    10861 gcggcaccgc cgagattgcg ggaccaagca cagcgacaac cgcgacaaga agcagcagtc
    10921 cagcagcgac gcccgacaac gctgaacgcc aatctcttgc cgtgcccatg gcgagaacga
    10981 cagtggtcgc ctcgacagcc tcaaccgcac acgcaaggaa caccgtgaga aacagggcag
    11041 cgctgttcac gtgccgactc ctttcggtcc gaggctttgg tcatccgcaa tgattccagt
    11101 gcttcctgag tacaacgacg gtcgctgacg tcgcgtcggc tcaccaccgg tcgaatccac
    11161 gcacgtaatc ccgacccaaa tcggctggtg acgtccggat ttggccgggc cggaccaagc
    11221 tgaaacgccc gaaagcctca gtcgtgcaga tcgaaaatct accgctggca cggacgctgc
    11281 aagtacggct ggcaggaatc ctcagcgggg acgagcagtg acgacatgcg ttggaccgag
    11341 cgcgatgctg cagacaggag tagaccggcc aacaatcgga ggactaagca catatgaacg
    11401 ccagcaaccc cgacgggttc catcccggac tgaacgattc cagcgctacg catgtcgacc
    11461 ggtcgttgcg cacgcgagtg catcacgtca aggcttcaga gctgagttcg gcgactacat
    11521 cttcgtgccg ccatacgttc cgcatcgcga agaagagaat cccgacaccg acaactccgc
    11581 ggtgatcgtg atcgctcgca gcacgcagga ggcaatagtg gtgaatcttc ctgcgttgga
    11641 cgcgctctca gatcagccca agtgatgtgc cggcgtcatt ctggccaggc ggactgagca
    11701 atgatgctga caaagtcctt gcgtcggaag tccgcatcaa agtgcgcaat gacgtcgtcg
    11761 ttcatcgtgc cgaatgtcgt ctccggccga tcccgcatgc cgtcgtagaa ggcgcgctga
    11821 atccgattct tgaagtcggg gcgcgggtgc gcagcgatga cggccgcgag ttgctcgtcg
    11881 ctgatctcat agatttgttg gccgagtacg tcagtctcca cgccaagggt gacgagatgg
    11941 atctccggtg cgaggtgttc ggggatgccc ggagtggtgt gaagcgcgat cgacaaccag
    12001 accattcggg cctcggcagg agagtatccc tgatcgagca ggaattgctc ggcgatatcg
    12061 gcaccgtcga cctcgaatcg ctgatgcgcg gtgcggtatt tcggtgtcaa gccgaggtca
    12121 tggaacatgg caccgacgta gctgagttcg gggtcgggcg aatgcctgcg gcggcgtgac
    12181 ttgagcatgc cccacaggta cacccgccgc gagtgatgaa acagcagatc gtcctccgca
    12241 tcaaggacca gttcggtagc ggccttgacg acgacgctat ccggaatggt cactccagca
    12301 attatttccg tcatttctct gtccttgtcg tggcgcgggc caacgtgatc tcggctgcga
    12361 accccgcgat gtagatcacc agttgtgctg ggagatgggc acgaatcatc ggtccgcgcc
    12421 ccccacgagt gctcccgcgc gcacaccctt ctcaacgcgc tcgcccagat ccatgtctac
    12481 attgcgaaaa tattcgaatg cgcgctgcag gactggctcg gtcactccgt caagtaggtg
    12541 accgacgatg ttgtcgacca accgttcccg ctcggcgtcg tccatcacgt cgcggaccag
    12601 ggtgccggcc tgaccccaat cgtcgtcatc ggaccgcaaa gtgtaagcgg tgcggaccat
    12661 ttcgccgtcg gtgtgccagc cggccggctc accgtatcgc tcggtgtcgg cgcgcggccc
    12721 gcccttggag ttcggcgcgt agaccgggtc ggtggccagc tgataccgca tctgcccggc
    12781 ggtgttgtaa ctgtgcaccg ggcacttggg tgcgttgaca ggcagctggt tgtagttggc
    12841 gccaatgcgg tatcgctgcg cgtcggcgta ggcgaacatc cgccccagca gcatcttgtc
    12901 cgggctcgcg ccgatgccgg gcaccagatt gctgggcgac caggcgccct gctcgatctg
    12961 gctgtggttg tcggtgggat tgcggtccag cgtcagtcgg cccacctcga cgagcggata
    13021 gtcggcgtgc ggccacacct tcgtcaggtc gaaggggttg aaccggtatg ttttagcatc
    13081 ctcgaacggc atgatctgca tcttgagcgt ccacgacggg tagtcgccgt ggttgatcgc
    13141 ctcgaacaga tcgcgagtgt ggtaatcggt gtcgaccgac accaacgcgt cggcctcggc
    13201 ctgggtcagg ttctgaatgc cctgatcggt gatgaagtga tagcgcaccc agaacctctc
    13261 cccggcggcg ttgacccaca tgaaggtgtg gccgctgtag aggttcatgt accgccagct
    13321 gcgggggatg ccgcgatcgc ccattagcca ggtgacctgg tgcgcggatt ccggagacag
    13381 cgtccagaag tcccactgca tgtcgtgatc acgcaggttg ctgtcggcgc gacgcttctg
    13441 ggaacggatg aaactgggga atttcaacgg atcgcgcaag aagaacaccg gggtgtcgtt
    13501 tccgacgatg tcgtaattgc cctcggtggt gtagaacttc agcgcgaagc cgcgcgggtc
    13561 gcgccaggta tctgggctgc cacgctcacc ggccaccgtg gagaaccgga tcaccgtctc
    13621 agtcgtggtg ccgggctgaa acaccgcggc cttcgtgaac gcgctgacgt cgtgggtcac
    13681 ctcgaaccgc ccgaaggcgc ccgagccctt ggcatgtggc tggcgttcag gcacccgctc
    13741 ccggttgaac tgtgccagct gctcgatgag gtagtgatcg tgaagcagaa tcggcccatc
    13801 cgggccaatg gtcagcgaat gctcatcgct ttccaccggg ataccggcat tcgtcgtcgt
    13861 cggcggtgta ggttgcatgt cggtcatggt cttgcctttc ggtagtggtg aaatactctg
    13921 ggaagctgga gaattggtgc cggtgaccgt gttagtcgtc cacatccggc acggtgagca
    13981 gactgcggtc gaggatctca gcgacccact cgatcaggcg gccgatgatg ttatgcatac
    14041 tcgttccttt ggtcgatccg ctgcagcgcc atccgctgca ggtgatacct tctagcgact
    14101 gtccttgcac gacaacccat tccgtggcgc aacgccggag tcgtgaggag acgcagtagc
    14161 gcccccgcca tccctgacac atcgccccgc cggtagatca gtcccgcgct cgccggcacc
    14221 accaatcgca cgattgtcgg gtcggcctgc gcaaccaccg gacggccggc gcccagcacg
    14281 tcgagcagcg tggtgtatcc cgtggcattt tccgggacga cgacgtctgc ggcggcgatc
    14341 aggtatggcc aacggtcctc ggtgagatcc ctgatcacgt gcacacttga tccggcgcct
    14401 tgtgcgaagc ttcgccgcaa gaccgtcgcg cggtattcgc cgcccccgag gaggacaagc
    14461 tgtgcccgac atcgcgaccg cacggttgcg aaggcggccg ccagttggtc ggcgtctgct
    14521 tggtcgtcga acggacctgt agcgaggatg gttggaagac ccgccaggcc caccagcgaa
    14581 cgggcggcca tggggttgag cgccgcggcc ggtgggaccc tcgagatggt cacttgtcgt
    14641 aggggttcag ggcgagaaat gtgactcccg cggcta
//
LOCUS       DASRJA010000057        20174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1399993,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000057 DASRJA010000000
VERSION     DASRJA010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20174
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399993"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VFC01_02425"
     CDS             <1..936
                     /locus_tag="VFC01_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZN78532.1"
                     /translation="RIALIVGDCVGHGLAAATVMGQLRSACRALLLEHPSPRAALTAL
                     DRFAAQLPGARTTTAFCAVLNPDTGELVYSSAGHPPPVLVHADQTVELLDGGRAIPLG
                     LGFSGERPQAHRSLPARATLLLYTDGLVERRREPLDRGIARAADLVVDNRAAVLDELA
                     DLIMSRIAPKDGYDDDVAMLLYRQPAPLQVAIPADPGELAATRAVLRSWLDRAGVSPD
                     QALNVLVAAGEALANAIEHGHRHQPGGAVRLQAMALGDSVRVTVADDGSWKTPQPAAD
                     PHRGRGITLMRGLMQDVEISPGIDGTTVAMHTRIA"
     gene            936..1241
                     /locus_tag="VFC01_02430"
     CDS             936..1241
                     /locus_tag="VFC01_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN78533.1"
                     /translation="MRTPVTLSTGRDTDGRLVVSATGELDMSNIDTFNRALTDALTQA
                     NGSSVEVDLRGVEYLDSGAINVLFPYADRVHLVANRILMPVLKVCGLTDVMSIEPAG"
     gene            complement(1245..1538)
                     /locus_tag="VFC01_02435"
     CDS             complement(1245..1538)
                     /locus_tag="VFC01_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HZN78534.1"
                     /translation="MFRMTVEDVFVIRNRGVVATGRVQSGILHVGDTVQIDGTVEARV
                     DGIEVFRKSIDEAKAGDNIGVLFKGIEKSQLDRGSVLTAAGEFPSIEQTGFVV"
     gene            complement(1557..2918)
                     /locus_tag="VFC01_02440"
     CDS             complement(1557..2918)
                     /locus_tag="VFC01_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN78535.1"
                     /translation="MVDLPALGPDGEYRTRSREIIKDTSGVPVAESNLVPRLFVHRSI
                     DAQRKLRPLPVTERDAALTKAADVFVSSTIAGLDFDSYVDLTCRVTGLPMTAAREGAH
                     TVAESLTTACDAVRPARPSGASSDWRDVSSAGGAVWVRRGEVLAVHAPGNAPGVHGLW
                     PQALALGYRVAIRPSRREPFTAHRLIHALRQAGFRAADALYLPTEHAVADEVIEAADL
                     AMVYGGQDVVARYACDPHVLTNGPGRTKILITAECDWREYLDVIVDSIANLGGMACVN
                     ATAVLYEGDAAPLAHAIAERLAAIEPLTNNDERAVLPTTSIDAARAIADHLAARAAGT
                     VPVLGADQVVADLGGGFAALRPAVHLLATPDPTKLNVELAFPCVWVSPWTRAHGIAPL
                     RNSLVLNAITNDDDLVDELVSEPTVTNVYRGRHATHHAAPHVPHEGFLADFLMRNKGF
                     IGG"
     gene            complement(2934..4025)
                     /locus_tag="VFC01_02445"
     CDS             complement(2934..4025)
                     /locus_tag="VFC01_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016888702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenazine antibiotic biosynthesis protein"
                     /protein_id="HZN78536.1"
                     /translation="MADIDFSLLDVPRDAPPEDAEAYLRAAIAWHFNADTGSPFWLRT
                     AQTLDFDPLTDVQTYADLRRFPNLVDELRSVPVEDLIPRGYGTPPPIPRIFESGGTTG
                     APKRTAQLPDWVEQVTRWQVEDFAAGGFVPGRGLLCLMPSGPHGVGHFDRVVAERLGS
                     VCHAIDLDPRWVKKLAARNATSEMAAYVDHVLEQASHILETQDVVNLHATPPLVEAIA
                     RDDSLVDIVNRKIRYLLLSGAHVDMDTLDLLRQIFPNTAIAMAFGSTMVLSQAATRID
                     GDSIAFDPRSPYVVFWVIDPDTGDEVAYGERGQVVMNHISKSMFIPNNLERDTAIRMP
                     GPDGQVGDSVSEVAPVAIFDGEPVIEGVY"
     gene            complement(4065..4858)
                     /locus_tag="VFC01_02450"
                     /pseudo
     CDS             complement(4065..4858)
                     /locus_tag="VFC01_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(4882..5883)
                     /locus_tag="VFC01_02455"
     CDS             complement(4882..5883)
                     /locus_tag="VFC01_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78537.1"
                     /translation="MRARTYNQSHLARAHSGGRRISIYWTWSYPWEAQRDPASMENRF
                     STITEVRNVLWPAYETPEYDAANFLQGIAGTLELFHRSTLAFQELAEEATEHPVAVFQ
                     RIDQAGYKLPIDERILADTDTLMVFGLDHLLSEQEAAPEEIAAITEWLKREGTCLLLA
                     PHHDVGFTDDYAQRQVEYEHHGDRLVPRQQRFGQYTRSLMKALDVPVHNKWGLRPAVV
                     EGTKEIAPLTGFRDLDTPRLLDNVTTFNFHPHLPHYELTAPEGKLVQVLGRQRVDPNR
                     PHPFSEAGNTEFNALIWMPPAGQRAGDIVLIDSTHFTTLFGGTDSLRNLWHNLATMK"
     gene            complement(5894..7213)
                     /locus_tag="VFC01_02460"
     CDS             complement(5894..7213)
                     /locus_tag="VFC01_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HZN78538.1"
                     /translation="MLELDDIQHILLTRTPAITGRYEFLSFDTPEGGRAWLTELLDKV
                     QSAADVHATMDSSDRWVTLAFTWNGLRALGVPEESLATFPDEFREGMAARASILGDTG
                     AAAPEHWVGGLAGEDLHAIAILFSRTDEQCRRSIEEHDKLLARTDGVRSLSHLELNAT
                     PPFNYAHDHFDFRDRLSQPVMKGSGEEPTPGSGAPLEPGEFILGYPDENGPVANLPEP
                     EALSRNGSYMAYRRLQEHVAAFRDYLRENADTPEGQELLAAKFMGRWRSGAPLVLAPD
                     KDDPELGADPMRNNNYNYKEMDPFGYACPLGSHARRLNPRDTAHNMNRRRMIRRGATY
                     GPALPGDAPDDGVDRGIAAFIICADLVRQFEFAQNVWINDKTFHELGNEHDPICGTQD
                     GAMDFTVPKRPIRKVHKGIPAFTTLKGGAYFFLPGMNALRYLATLSH"
     gene            complement(7216..7770)
                     /locus_tag="VFC01_02465"
     CDS             complement(7216..7770)
                     /locus_tag="VFC01_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78539.1"
                     /translation="MTDQDWTKPAAMAIPKEGYFELERGRYGPIYPRTPACYGFSIIA
                     KVKEGREEAVRAYGKTIEDTIASSPEALAPLRLHYLRWVLFDVGSGLHFQYQGIFDTD
                     FDKYTEDAVQLFSATGITTVFTNLEGFPEDWKENPEAFVKFVREHQCPSFLEYGEYPY
                     VTADEIKKALRLKAAFSTMLDQMQ"
     gene            8049..8348
                     /locus_tag="VFC01_02470"
     CDS             8049..8348
                     /locus_tag="VFC01_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78540.1"
                     /translation="MRRSQRYLILGLTLTAVPVGIVTAAPASADCTSAGAATVCAQGT
                     VRGGGPSAPSAGPVYPSYCADPWYCDDGWDVDVVLPPIDIGRPGRPGGGGGIGPR"
     gene            8389..8733
                     /locus_tag="VFC01_02475"
     CDS             8389..8733
                     /locus_tag="VFC01_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZN78541.1"
                     /translation="MFIRSLIGAGVIAGATLLGTAATAAADPPNCTAADLAGVMSGVN
                     AGMSSYLFTHPDVNAFFTSLKGKERDQMRTELTAYIDANPQVGDEIRAVRQPASDFRD
                     RCNAPVPDGPMG"
     gene            complement(8743..9342)
                     /locus_tag="VFC01_02480"
     CDS             complement(8743..9342)
                     /locus_tag="VFC01_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN78542.1"
                     /translation="MGVSNQVLGLRDRKKIQTRMTIRNEAMRLIEENGYANTTVEQIA
                     EAAEVSPSTFFRYFPSKEMALMANDLDLVTIRALEQQPADLPSLQAFRRALEITMATL
                     SEDEWRFERARLRMVLSIPELKAAQFEEYRSTVAALTAADCRRTGRAPDDFEVRVFVG
                     ALAGGLMAVLDQASGVPERMYRALDFMEAGMPLRGNGDS"
     gene            complement(9369..9968)
                     /gene="lysE"
                     /locus_tag="VFC01_02485"
     CDS             complement(9369..9968)
                     /gene="lysE"
                     /locus_tag="VFC01_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter"
                     /protein_id="HZN78543.1"
                     /translation="MSSPLVLGFIASFTLIAAIGAQNAFVLRQGIRREHVLPVVALCT
                     VSDIALISAGIAGVGALISAHPSALNIAKFGGAAFLVGYGLLAARRAWRPSSLMPSEA
                     TPARLIDVLLTCAALTFLNPHVYLDTVVLLGALANEHRDERWLFGVGAVTASAVWFVS
                     LGLGARQLAGLFARPLTWRILDGLIAVMMIGLGVSLALS"
     gene            10230..10967
                     /locus_tag="VFC01_02490"
     CDS             10230..10967
                     /locus_tag="VFC01_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016895338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA-like heteromeric transposase endonuclease
                     subunit"
                     /protein_id="HZN78544.1"
                     /translation="MSVGVDVDLGSAVMSYRPAAGEERSLPAQRVSAAALFECVPWRT
                     FRWYFGQRHYSGTWWSSTMCDHVIYESRLELSRLLLADFDPCVRQMIAQPFMVTATVD
                     GQPRRHILDYLWATIDGPVVVDVVRAERLNHPGVALLCQWTRDVVESLAWGYQVLSEP
                     PPTRLESVRFLAGYRRNWLIHPEILEEMRSRTTDLVGMSIADAEREFAYPSSLVRPAL
                     MHLLWCHELEVDLDRPLRPSRILEAPR"
     gene            10964..13096
                     /locus_tag="VFC01_02495"
     CDS             10964..13096
                     /locus_tag="VFC01_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HZN78545.1"
                     /translation="MKRAGVRVGVGTRFSYDGEIVEIVESHVVDGDIEVLVKDLRAQT
                     VSRFALNELMLSERSRLLTADLAEEVSEFEGDPATVKWSAASDAVRREACARAAHVRE
                     VITGYRSGHAESALRGEPKAAYRPALPKGKRVAAKAKELGIGLRTLQRWVADYSKYGE
                     AGLISSKAVQPDLGSTRFAVFEQTALDIMVEHTDFSKPTKHHIIAHACARITKTYGEG
                     AVPLPSDSRAYDILNRLERKHRIFSGNAKRNRDIAARSLRAYGKLHPHRPGEYLLMDT
                     TRLDVFAMDPQTLRWLGVDLTVGMDWYSRCITGLRLTPVSTKAIDASAVLYQSFRPMP
                     VGDDWPDHASWPPHGIPRSVLVESKVLDPRSALAASPAIVPETIVVDHGKIYVGETLT
                     SACRQMGISIQPARIREGRDKGPVERFFKTIREGFLQELPGYKGPDLYSRGAAPEKGA
                     FLFIDEIEALLREWVATVYHHKPHDGVGEAGLWALEMSPTQMFEHGLTRAGYIEAPRD
                     PCLAFHFLDVAWRTIQHYGVEINHRVYRASVLVDYVDQKSPYKHRDGKWPIHINPDDI
                     RNVYFFDLKKTRRWCVLVWTEAAVLRGPMTEDGLAFARQLARKKHRYFDNKLALAELL
                     QRRNLSQGRTQAERIAALRVSREQSSLELDLAAAIEVSQLPTVINAIEEQAAMDTVAE
                     EAAAFGDDLDDTPAAIYDGFYDDVLEDV"
     gene            13093..14220
                     /locus_tag="VFC01_02500"
     CDS             13093..14220
                     /locus_tag="VFC01_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN78546.1"
                     /translation="MSTATAPTQVEAKRPNLDNLTLARKEGWHDYVESPKRVSPELLS
                     KREIRRLNGPAADIYNRRRADWHNNLGPFKTPQLAAVHDALWDIMDSNAQDGHRAKGA
                     VAIDGYPGLGKSCAAEEFAKQFHQREIVRHGEFTRAGDERWPVCRIGMRGNTSMKDFN
                     AALCDFYAHPGRRGATAEQLGRWALDCVLSCETRLLIIDDLHFLHWQHHGGVEISNHF
                     KYIANEFPLTLLSIGVGLGERGVLLEGTSYDDAVLAQTMRRTTMLDMQPYAITTERAR
                     RQWRTLLVTIEQRLILGSKHPGMLADGLSDYMFIRSTGHIGSLMELIRRGCAKAIRTG
                     VETLDQKLFDTIKIDSAAERARRELEVAFRTRRLTTRRQLR"
     gene            14217..16784
                     /locus_tag="VFC01_02505"
     CDS             14217..16784
                     /locus_tag="VFC01_02505"
                     /inference="COORDINATES: protein motif:HMM:NF018259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HZN78547.1"
                     /translation="MTRTLPVRAAPVSGEALDSWLETIAARCGAPLGDILRAVGIKSP
                     IACGPGWLLRLSDEERHQISCATGIDETAIAAMTLAHYNGTALEIDYERHRLNHSFPF
                     GARSWSRYCSQCLAETHGRWQLHWRLGWTFACTRHCCLMADHCPNCGRRQRKTHSRYN
                     TVPLPGHCSGTAGSSCGENLTSASVLQLPTHHPVIEAQKTLFEMIGDDRAAFGLYAAR
                     PCSAREALTDIKALASRILAYAADNGLRAIEQSNLLPQYGVKRGTGEGVHWRYRQATT
                     MMNAPQIAVEAAVAITAALGVLESADIALAADKVRWLIAGQLHRGPPSALQRYARDGG
                     LVAAVLLKVFAPPVGSSLTGSHMQLRYRTTLPVPTAPESGKTRLHRTAHRLPSMLWPN
                     WSIRLQLRPSSYWYLRCLLSCATLLVGTTARVEDATAALGGVIDADGAKNAISQLQQS
                     PHWAAVCSAATRLSEYLNDTPAPIDYRRRRRLDYTALLTQERWMQICRQTGALPGKSE
                     GQKALIARAHLYQKVSGRPAHELLQGTPPATRVDLVQRIANFPRRITPELARLLDQES
                     RNFLRAKHIDEPITWSPPLELVNDLDLPEPDPAQTTIDDLHALAEDMTISITEIAGRL
                     NTSAHAVRYLLDKHPTQPRNRSAVESARAHTCPTITADVLQELYVQQGLTINQIAAQF
                     GTTRFMVKRLLLRDGLPIRHRPPPPSGDWLYEQHIIERRTLTDIAMDTGVQARAVHVW
                     KKQHQLASRTDKSSFRRRPMSQGTARQVLEPWYAAHAAASWLTNFTETLRYPTMQAAA
                     AELIINPSTLQYRITALERIFGEPLVFRARHGLALRPTNLGEQVAEAISAINPEATL"
     gene            17714..18283
                     /locus_tag="VFC01_02510"
     CDS             17714..18283
                     /locus_tag="VFC01_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78548.1"
                     /translation="MQLPQAHPALTAQATIQALLDGTRTELAIYGCVAPTCSSMLGDL
                     RVLAQWVIYAVNQAQLDGHLPTDLSSAVALHRQSTSWPYGRHWRGARTVPSGLDTAAG
                     VSLALGVISLPNTDTTVSTLRQLMHDAHNGGPYQTPMSRRAALSPALTSVLEAAIVAH
                     VADRKLRSRVARKLASLSSVPAGSTPLPR"
     gene            18319..19248
                     /locus_tag="VFC01_02515"
     CDS             18319..19248
                     /locus_tag="VFC01_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HZN78549.1"
                     /translation="MGKDQPTHLDPHQLAALAAVVEEGSFDAAAETLHVTPSAVSQRI
                     KTLEQHIGQVLVVRQKPCRPTAAGVPLLRLAAQMALLEAEVLAESAGRSAQLTRIAIA
                     VNADSMATWFTAVLGTLSDVLFDIRIEDQDHSARLLREGAVMGAVTTERSAVPGCRVK
                     SLGVMRYVPVASAAYVERYLSDGFTRESVKAAPSLAWNRDDALQDMLVRKIFRRAIAR
                     PIHYVPTAEGFSAAVRAGLGWGMFPEKLAARDLTQGVFVHVADTYLDVPLYWQCWKMD
                     SPLIETITDAVQSAAQVLHRHRTKPRTSSPNRS"
     gene            complement(19366..19626)
                     /locus_tag="VFC01_02520"
     CDS             complement(19366..19626)
                     /locus_tag="VFC01_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN78550.1"
                     /translation="MPTRPSQNSPERVALWEQRRRVVGQRIRALRTERGMTQEALALL
                     SGVTRNVLIDVEHGRRSLLYERLFDLADALDVPAAELLVADD"
     gene            19822..>20174
                     /locus_tag="VFC01_02525"
     CDS             19822..>20174
                     /locus_tag="VFC01_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78551.1"
                     /translation="MDELPSSLREYLDLWCADLLDPLTAARRDQVCSLVESSYAGGPW
                     PPRLLVQRMCELNTGCIDIDAFVGDVTTRWGCSIDDAVRMLSAEDCDDRANAIVLLGR
                     YPDTTELVEKVTAAH"
BASE COUNT         3721 a         6376 c         6409 g         3668 t
ORIGIN      
        1 cgcatcgcgt tgatcgtcgg cgactgtgtc ggccacggcc tggccgccgc cacggtcatg
       61 ggtcagctca gaagcgcgtg ccgtgcgctg ctactcgaac acccaagtcc tcgcgccgcc
      121 ctgactgcac tggaccgatt cgctgcacaa ttacccggtg cgcggaccac gacggcgttc
      181 tgcgctgtgt tgaaccccga cacaggcgag ttggtgtatt cgagcgccgg gcatccaccg
      241 cccgttctgg ttcacgccga ccagaccgtc gagctgctcg acggcggccg cgccatccct
      301 ctcggtctgg gcttctccgg cgagcgcccg caagctcacc ggtcgctgcc cgcgcgcgcg
      361 accctgctgc tctacaccga tggtttggtg gagcgtcgtc gtgaaccgtt ggatcgcgga
      421 atcgcccgcg ccgccgacct ggtggtcgac aaccgtgcag cggttctcga tgaactggcg
      481 gatctgatca tgtcgcgcat cgcgccgaag gacggctatg acgatgacgt ggccatgctg
      541 ttgtaccgcc agccggcgcc actgcaggtc gccatcccgg ccgaccccgg cgagttggct
      601 gcgacccgtg cagttttgcg cagctggctg gaccgggccg gggtgagtcc cgatcaggcg
      661 ctgaacgtac tcgtcgccgc tggagaagcg ctggcgaacg ctatcgagca cggtcaccgc
      721 catcaaccag gaggagcggt gcgcctgcag gcgatggcgc tgggcgacag cgtgcgggtg
      781 accgttgccg acgacggttc gtggaagacg ccacagccgg cagccgaccc ccatcgggga
      841 cgcggtatca cactcatgcg aggcctcatg caggatgtgg agatctcgcc cggcatcgac
      901 ggcaccacgg tggcgatgca cacgaggatc gcctgatgag gacgcctgtg acgttgtcca
      961 ccggtcgtga cacagacggc cggcttgttg tgtctgcgac gggtgagctg gacatgagca
     1021 acatcgacac cttcaaccgg gcgctcaccg atgcgctgac acaggcaaat ggcagctctg
     1081 tcgaggtgga cctgcgcggg gtcgaatacc tggacagtgg tgccatcaac gtcctgttcc
     1141 cctatgccga tcgtgtgcac ctggtcgcca accggatcct catgccagtg ctcaaggtct
     1201 gcgggctcac cgacgtgatg agcatcgaac ctgcgggctg atcgtcagac gacgaagccg
     1261 gtctgttcga tggacggaaa ctcgcccgcc gcagtgagca cagagccgcg atcaagctgg
     1321 ctcttctcga tgcccttgaa cagcacaccg atattgtcgc cggccttggc ttcgtcgatc
     1381 gacttgcgga agacctcgat gccgtccact cgcgcctcga cggtgccgtc gatctgcacg
     1441 gtgtccccga cgtgcaggat gccgctctgt acacggccgg tcgcgacgac gccgcggttg
     1501 cggatcacga acacgtcttc gacggtcatt cggaacatgt caggagtgtg ccaggttcag
     1561 ccgccgatga agcccttgtt gcgcatcaag aagtcagcca ggaagccctc gtggggaacg
     1621 tgcggcgcgg cgtggtgcgt ggcgtgccgg ccgcggtaca cattggtcac ggtcggttcg
     1681 ctcaccagtt cgtcgacgag atcatcgtcg ttggtgatcg cgttgagcac cagtgagttt
     1741 cgcagcggtg cgatgccgtg cgcccgcgtc catggcgaga cccacacaca ggggaaggcc
     1801 agttcgacgt tgagcttggt cggatcaggt gtggccagca ggtgcacggc tggtcgcagg
     1861 gcggcgaatc cgccgccgag gtcagcgacc acctggtcgg cacccagaac gggcaccgtc
     1921 cctgcggccc gtgccgcgag gtggtcggcg atcgcccgcg ccgcgtcgat ggaagtcgtt
     1981 ggcagaacgg cccgttcgtc gttgttcgtc agcggctcga tcgctgccaa ccgctcggcg
     2041 atcgcatgcg ccaacggtgc ggcgtcacct tcgtacaaca ccgcggtggc gttgacgcac
     2101 gccattccgc ccaggttggc gatcgagtcg acgatcacgt cgaggtactc gcgccagtcg
     2161 cactccgcgg tgatcaggat cttggtgcgg cctggcccgt tggtgagcac gtgcgggtcg
     2221 catgcgtacc tggcgacgac gtcctggccg ccgtagacca tggcgaggtc ggccgcctcg
     2281 atgacttcgt cggcgacggc gtgttctgtc ggcaggtaca gcgcatcggc ggcccgaaaa
     2341 cctgcctgcc gcaaggcgtg aatgagccgg tgagcggtga acggttcgcg ccgcgatgga
     2401 cggatcgcca ccctgtagcc cagcgccaac gcctgcggcc ataggccgtg cacgccgggc
     2461 gcgttgccgg gcgcgtggac cgcgagcacc tcgccccgcc gcacccacac ggcgccgccg
     2521 gcactgctca cgtcgcgcca gtcggagctt gcaccggacg gacgggccgg acgtacggca
     2581 tcgcaagcgg tggtgaggga ttcggcgacg gtgtgggcac cctcgcgggc cgcggtcatc
     2641 ggcaggcctg tcacccggca ggtgaggtcg acatagctgt cgaagtcgag tccggcgatc
     2701 gtcgacgata cgaagacgtc ggcggctttc gtcagcgccg catcacgttc ggtgactgga
     2761 aggggccgta gcttgcgttg ggcgtcgatc gaccggtgaa cgaatagcct tggcacgagg
     2821 ttcgattcgg caaccggcac gcccgaggtg tccttgatga tctcgcggct gcgggtgcgg
     2881 tactcgccgt cgggcccgag cgcaggaagg tcgaccagat cgcctgtcat cgttcagtac
     2941 acaccctcga tgacgggctc accgtcgaag attgcgacgg gtgcgacctc gctgaccgaa
     3001 tcgccgacct gcccgtcggg acccggcatc cggatcgcgg tgtcccgttc caggttgttc
     3061 gggatgaaca tgctcttgct gatgtggttc atcaccacct gaccgcgttc tccgtacgcg
     3121 acttcgtcac cagtgtccgg atcgatcacc cagaagacca cgtacggcga gcgggggtcg
     3181 aaagcgatcg agtcgccgtc gatacgggtg gccgcctgcg acagcaccat cgtgctcccg
     3241 aatgccatgg caatggcggt gttcgggaag atctggcgaa gcaggtcgag ggtgtccatg
     3301 tccacatggg cgccgctgag cagcaggtac cggatcttgc ggttcactat gtcaacaaga
     3361 ctgtcatcgc gggcaatcgc ctcaaccaat ggtggtgtcg catgcaggtt tacgacgtcc
     3421 tgagtctcta agatgtggct cgcctgctcc agcacatggt ccacgtaggc ggccatctcc
     3481 gacgtcgcgt tgcgggccgc gagctttttg acccaccgcg gatccaggtc gatcgcgtgg
     3541 cacaccgacc cgagccgctc ggcgacgacg cggtcgaagt gcccgacgcc gtgcgggccg
     3601 ctcggcatca ggcagagcaa cccgcgaccc ggcacaaacc cgcccgcggc gaaatcctcg
     3661 acctgccaac gggtgacctg ctcgacccag tccggaagct gcgccgtgcg tttcggcgcg
     3721 ccggtggtgc cgccggactc gaagatgcgg gggatgggcg gcggtgtgcc gtagccgcga
     3781 ggaatgaggt cttcgacggg gacactgcgc agctcgtcga ccaggttggg gaaccgacgt
     3841 agatcggcgt aggtctgcac gtcggtcagc ggatcgaagt cgagagtctg cgccgtgcga
     3901 agccagaacg gtgagccggt gtcagcattg aaatgccagg cgatcgcggc gcgcagatag
     3961 gcctcagcgt cctcgggagg cgcgtcgcgc gggacgtcca gcagggagaa atcgatgtcc
     4021 gccacgcggc catcgtgtcg caggaggttg tccagtaggt gaactcagga atgagcgagc
     4081 cgcttgttga cgacgcgcgt cagccatccg ggagagaagc gggagcctgc cgccatggcc
     4141 ttggtctgcg aaccgacggg aaagtgcacc tggtggatgg cgcggcgcag ccacgacggt
     4201 tcgacggccg tggcgatcgc gtcggcgacg tcctgtgcgg tcagcttgat gcccagcgat
     4261 tccgtcgtgc cagtcttgat gtgatcggtc atcgcggtct gcacgtagag cggccacatc
     4321 gcgatcaccc ggatcccgta ttggtgccat tcgatgtcga gcgcctcggt gatgccgcgg
     4381 acgaagaatt tggtggcgct gtagttggcg agttcggcct ggccgtagat cgccgacgcg
     4441 gacgcgagat tgaccacgac cgagttggca gtgtcgcgca ggtaggaaat gcggcgtgca
     4501 gtccgttgac gacgcccttg gcgttgatgt cgatctcttg cagatgcttc gcgacgtcga
     4561 tgtcctcgaa acgaccagcg aggaggatgc ccgcgttgtt gatcatcacg tcgagccgac
     4621 cggcagactt ggcgaattcg cctacccgtt gcgccatttc ggtgggatcg gtgacatcaa
     4681 gatgcccgac aatcgcggtt ccgccaagtt ggtcgatctc atcagccagc gacttcagac
     4741 cgacctcgtc gatgtcgtag ccgccgacgg tatagccctt ctttgcgaag gtcagggcgg
     4801 tggcccggcc gatccccgcg gcggcgccgg tgatgaacac ggctctgttg ttgggcatca
     4861 cgcgatgatc gcccatgagg ctcacttcat ggtggccagg ttgtgccaga ggttacgaag
     4921 gctgtccgtg ccgccgaaca acgtggtgaa gtgggtggag tcgatgagca cgatgtcgcc
     4981 cgcgcgttga cctgcaggcg gcatccagat cagcgcgttg aactctgtgt tgccggcctc
     5041 gctgaacgga tgcggcctgt tcgggtccac cctctgacgt ccgaggacct gcaccaactt
     5101 gccttccggc gcggtgagtt cgtaatgcgg taggtgcggg tggaagttga aggtggtgac
     5161 gttgtcgagc agtcgcggcg tgtcgaggtc gcggaaaccg gtgagcggag cgatctcttt
     5221 ggtgccttcg acgaccgcgg gccgcagccc ccacttgttg tgcaccggca cgtcgagcgc
     5281 cttcatcagc gaacgcgtgt actggccgaa ccgctgttgg cggggaacga gccggtcgcc
     5341 atggtgttcg tactcgacct gtcgctgggc gtaatcgtcg gtgaagccga catcatgatg
     5401 cggtgcgagc agcaggcagg tcccttcccg cttgagccac tcggtgatcg ccgcaatctc
     5461 ctcgggtgcc gcctcctgct ctgacagcag atggtcgagg ccgaacacca tgagcgtgtc
     5521 ggtgtcggcc aggatgcgct cgtcgatggg cagcttgtag ccggcctggt cgatgcgctg
     5581 gaacaccgca accgggtgct cggtggcctc ctcggcaagt tcctggaacg ccagtgtgga
     5641 ccggtggaag agctccagcg ttccggcgat gccctgcagg aagttcgccg cgtcgtactc
     5701 cggcgtctcg taggccggcc acaagacgtt gcgcacctcg gtaatcgtcg agaagcggtt
     5761 ctccatcgac gccggatccc gttgtgcttc ccagggatag ctccacgtcc aatagatgct
     5821 gatgcgccgc ccaccgctgt gggcgcgagc gagatggctc tggttgtagg tgcgtgcgcg
     5881 catggtgatc cttctagtgg ctgagcgtgg cgaggtagcg cagggcgttc atcccgggca
     5941 agaagaagta cgcgccgccc ttgagcgtgg tgaaggccgg aatacctttg tgcaccttgc
     6001 ggattggtcg cttgggaacg gtgaagtcca tcgcgccgtc ctgggttccg cagatggggt
     6061 cgtgttcgtt gccgagttcg tggaaggtct tgtcgttgat ccatacgttc tgggcgaact
     6121 cgaactggcg gaccaggtcg gcacagatga tgaacgcagc gatgccgcga tcgacgccgt
     6181 cgtcaggagc gtccccaggc agcgcgggcc cgtaggtggc accgcgccgg atcatgcgac
     6241 gacggttcat attgtgggcg gtgtcgcgtg gattgagccg tcgtgcatgc gatcccagcg
     6301 ggcacgcgta accgaacgga tccatttcct tgtagttgta gttgttgttg cgcatcggat
     6361 cggcacccaa ttcgggatcg tccttgtcgg gagccagcac cagcggagcg ccgctgcgcc
     6421 agcggcccat gaacttcgcg gctagcagtt cctggccctc cggggtgtcc gcattctcgc
     6481 gcagatagtc gcggaacgcg gcgacatgtt cctggagccg ccggtaggcc atatagctgc
     6541 cattgcgcga caatgcttcg ggttcaggca ggttcgcgac ggggccgttt tcgtcgggat
     6601 agccgagaat gaactcaccc ggttccagcg gtgcaccgga accaggggtg ggctcctcgc
     6661 cggaaccctt catcaccggc tgcgacagcc ggtcgcggaa atcgaagtgg tcatgggcgt
     6721 agttgaacgg cggtgtcgcg ttgagctcga gatgcgacag gctccgaacg ccgtcggttc
     6781 gtgcgagcag tttgtcgtgt tcctcgatgg accgccggca ttgttcgtcg gtgcgggaga
     6841 acaagatcgc gatcgcgtgc agatcctcgc cggccaagcc gccgacccag tgttctggag
     6901 cggcggcacc ggtgtcgccg aggatgctcg cgcgggccgc catgccttcg cggaactcgt
     6961 cggggaaggt ggccagcgat tcctcgggca cccctagcgc gcgaaggcca ttccaggtga
     7021 acgccaacgt gacccagcga tccgagctgt ccatggtggc gtgcacatcc gccgccgact
     7081 ggactttgtc cagcagctcg gtcagccagg cgcggccgcc ctccggggtg tcgaaggaca
     7141 ggaattcgta gcgtccggtg atcgccgggg tgcgggtcag caggatgtgt tggatgtcgt
     7201 cgagttcgag cacggttatt gcatttggtc gagcatggtg gagaaggccg ccttcagacg
     7261 cagggccttc ttgatctcgt cggcggtgac gtacgggtac tcgccgtact cgaggaagct
     7321 ggggcactgg tgctcacgca cgaacttgac gaaggcctcg gggttctctt tccaatcctc
     7381 tgggaagcct tcgagattcg tgaacaccgt cgtgatcccg gttgcgctga acagctggac
     7441 cgcgtcctcg gtgtacttgt cgaagtcggt gtcgaagatg ccttggtatt ggaagtgcag
     7501 cccggacccg acgtcgaaca gcacccagcg gagatagtgc agacgcaggg gcgcgagcgc
     7561 ctcggggctg ctggcgatcg tgtcctcgat cgtctttccg tatgcgcgga cggcttcttc
     7621 gcggccctcc ttcaccttgg cgatgatcga gaatccgtaa caggcaggag tgcgcggata
     7681 aatcggacca tatcgccccc gttcgagttc gaaatagcct tccttcggga tggccatcgc
     7741 ggccggtttt gtccaatctt gatccgtcat atgtggcccc caatcacggt tttggtgtga
     7801 tgcgaacgta acaccgccgc ccagcacctg gcacataatt gacgggcgta acctgacgaa
     7861 ggcgcgcctg gggagggcgc ctaagtttat gatcgtgatt cttgcgatga caacgaagtt
     7921 ggtgatcatg aaattggtga attcgccacg gcgtccgggc gaatggaatt aggcttcgcg
     7981 cataaatccg gcacgttgtc gggggatatc taccgcggcg ggccacctac ttcggggagg
     8041 ggagtggcgt gcgaagaagc cagcgctacc tgattcttgg attgacattg acggcggttc
     8101 ccgtcggcat tgtcacggcg gcacctgcgt cggccgattg cacgagtgcc ggtgctgcca
     8161 ccgtgtgtgc acaagggact gtccgcggcg gtggtcccag cgccccgtcg gcggggccgg
     8221 tctatccgtc ctattgcgcc gacccgtggt attgcgacga tggctgggat gtcgatgtgg
     8281 tcttgccgcc catcgatatc ggacgcccag gccggccggg tggcggaggt ggcattggtc
     8341 cacgctgagc accgtccccg aatcacccag gaaaccaagg agtctcacat gtttattcgt
     8401 agcctcattg gcgccggcgt gatcgccggt gcgacattgc tcggcaccgc agcaaccgcc
     8461 gccgccgatc caccgaactg caccgccgcc gacctcgccg gcgtcatgtc cggcgtcaac
     8521 gcagggatgt cgtcttatct gttcacccac ccagatgtga acgcgttctt taccagcctc
     8581 aagggcaagg agcgcgatca gatgcgcacg gagctcaccg cgtacataga cgcgaaccct
     8641 caggtggggg acgagataag ggctgttagg cagcccgcct ccgacttccg cgatcgctgc
     8701 aacgcgcctg ttcccgacgg gccgatgggt taggggagtt catcaggagt ctccgttacc
     8761 cctcaacggc ataccggctt ccatgaagtc cagcgcccgg tacatccgct caggcacacc
     8821 ggacgcctgg tcaagtacgg ccatcaaacc gcctgcgaga gcgccgacga acacgcggac
     8881 ctcgaagtca tcgggcgcac gaccggtccg gcggcagtcg gccgcggtca gcgcggcgac
     8941 ggtgctgcgg tactcctcga actgcgcggc cttgagctcg ggaatggaca acaccatccg
     9001 cagccgggcc cgttcgaacc gccactcgtc ctcggagagc gtcgccatgg tgatctcgag
     9061 cgcgcgacga aacgcctgca acgagggcaa atccgcgggt tgttgttcga gcgccctgat
     9121 ggtgacgagg tcaagatcgt tggccatcaa cgccatctcc ttggacggga agtaccggaa
     9181 gaaggtgctg ggcgatacct cggcggcctc ggcgatctgt tcgacagtgg tgttggcgta
     9241 cccgttttct tcgatgagcc tcatggcttc gtttcggatg gtcatgcgag tttggatctt
     9301 cttgcgatcc cgcaaaccga gaacctgatt actgaccccc atggggtcat tgtgtcgatg
     9361 taaatggctc aggacaaggc caatgacacc ccgaggccga tcatcatgac agcgatcaga
     9421 ccgtcgagga tgcgccatgt cagtggccgg gcgaacagcc ctgccagttg gcgcgcaccc
     9481 agtccgaggc tgacgaacca gactgcgctc gccgtgacag cgccgacacc gaacagccaa
     9541 cgctcgtcgc ggtgttcgtt ggccaacgct ccgagcagca ccacggtgtc gagatagacg
     9601 tgcggattga ggaacgtcaa cgccgcgcag gtcaagagca catcgatgag gcgcgcaggc
     9661 gtggcttccg acggcatcag cgaggacggc cgccaagccc gtcgggcggc cagcagtccg
     9721 tagccgacga ggaatgccgc gccgccgaac ttggcgatat tcaaagcgct tgggtgagcg
     9781 ctgatgagcg cgccgacgcc cgcgatgcct gctgagatca gcgctatgtc ggacaccgtg
     9841 cagagcgcga ccacaggcag cacgtgctcg cggcggatcc cctggcgcag cacgaacgca
     9901 ttctgtgcgc cgatcgcggc gatcagcgtg aaggaggcga tgaaaccgag gaccaagggc
     9961 gagctcatgt cgtcgaccgt agaagtgaag ccaggatcag tacagctaat gtttcttcag
    10021 atgcattaga atcactaatg tgcaggtaga cggtcagcaa ctggctgcgt tcgccgctgt
    10081 aggttccgat tttccgtggc gaattccgat ctgaaatggc ggaagatggc ggatccgcca
    10141 taaagacagg aattctgccg gtttttgacc gatgaggtgc gcgtagtgac acgctgaacg
    10201 gtgcagtgcg tgcgctgcgt ggagtagcaa tgtcggtcgg tgtcgacgtt gatctgggtt
    10261 ccgcggtcat gtcttatagg cctgcggcgg gggaagagcg gtctttgccc gctcagcggg
    10321 tgtccgctgc ggcgttgttc gagtgtgtcc cgtggcgaac gtttcgttgg tatttcgggc
    10381 agcggcacta ttcgggcacg tggtggtcgt ccacgatgtg cgatcacgtc atctatgagt
    10441 cccggcttga gttgtctcga ttgctgctcg cggacttcga tccttgcgtt cggcagatga
    10501 tcgcccagcc gttcatggtg accgcaacgg tggatggcca gccaaggcgg cacatcttgg
    10561 actacctctg ggccactatc gatggcccgg tcgtagtcga tgtggttcga gcggagcggt
    10621 taaaccatcc gggtgttgcg ctgttgtgcc agtggactcg cgacgtcgtc gagtcgctcg
    10681 cttggggcta tcaggtcctg tccgagccgc cgccgacgcg gctggagagc gttcgcttct
    10741 tggccggcta tcggcgcaat tggctgatac atccggaaat tcttgaagag atgcgctctc
    10801 gcactacgga tttggttgga atgtccatcg ctgacgcaga acgggagttc gcatacccaa
    10861 gctcgctggt gcgcccagca ctgatgcacc tcctgtggtg ccacgagttg gaagtagacc
    10921 tagacaggcc acttcgcccg tcgagaattc tggaggcacc acggtgaaac gagcaggtgt
    10981 aagagtcggc gtcggaacgc gcttttccta cgacggcgag attgtcgaaa ttgttgagtc
    11041 gcatgtggtc gacggcgaca ttgaagtgtt ggtgaaagac ctgcgcgctc aaacggttag
    11101 ccggttcgca ctaaacgaac tgatgttgtc tgagcgttct cggctgctga cagcggacct
    11161 cgctgaggag gtttccgagt tcgagggtga tccggcgacg gttaaatggt ctgcggcatc
    11221 cgacgcggtg cgacgcgaag cctgtgcgcg ggccgcccac gttcgcgaag ttatcaccgg
    11281 ataccgatct ggccacgctg aatccgcgct gcgcggcgag ccaaaggccg cctacaggcc
    11341 ggcgctgccg aaaggcaagc gcgtggcggc caaagcgaag gaattgggga tcgggctgcg
    11401 aacacttcaa cgatgggtcg ccgactactc gaaatacggg gaagctgggc tgatctcgtc
    11461 caaggctgtg caaccggatc tgggcagcac acgattcgcc gtcttcgagc agacggcgct
    11521 ggacatcatg gtggagcaca cggatttctc gaagcccacc aaacaccaca tcatcgccca
    11581 cgcttgcgcg cgcatcacga agacctacgg cgagggagcg gtgccgctgc cgtcagattc
    11641 gcgggcatac gacatcctca accggcttga gcgcaaacac cggatctttt ctggtaacgc
    11701 caagcggaac cgcgacattg cggcgcgatc attgcgggcg tacgggaaac tgcatcccca
    11761 caggcctggt gagtatctgc tgatggatac gacacggctg gatgtcttcg ccatggaccc
    11821 tcagacgttg cgatggttgg gggtcgacct gactgtcgga atggattggt attccaggtg
    11881 tatcaccggg ttacggctga caccggtttc gacgaaagcc attgacgcat ctgcggtgct
    11941 ctatcagtca ttccggccga tgcccgttgg tgatgattgg ccggaccatg catcgtggcc
    12001 cccgcatggg ataccgcggt cggtgctcgt tgagagcaag gtactcgatc cacgcagcgc
    12061 actggcggcg agtccggcca ttgtgccgga gacgatagtg gtcgaccacg gcaagatcta
    12121 tgtcggcgag acactcacga gcgcgtgccg acagatgggc atctcgatac agcctgcccg
    12181 tattcgagaa gggcgagaca aaggaccggt ggagcggttc ttcaagacga tccgggaggg
    12241 cttcctacag gaactgccgg gctacaaggg ccccgatctg tactcccgtg gtgcggcgcc
    12301 ggagaaaggt gcattccttt tcattgacga gatcgaggcg ctcctgcgcg agtgggttgc
    12361 caccgtgtat caccacaagc cgcacgacgg ggtcggcgag gccggtctct gggcgctgga
    12421 aatgtctccg acacaaatgt tcgaacatgg cttgacccgc gccggataca ttgaggcgcc
    12481 ccgtgatccg tgcttggcct tccacttcct cgacgtcgct tggcgcacga ttcagcacta
    12541 cggcgtcgag attaaccatc gggtctaccg cgcgtcggtc cttgtcgatt atgtcgatca
    12601 gaagagcccg tacaagcatc gagacgggaa gtggccgata cacattaacc ccgacgacat
    12661 ccgtaacgtg tacttcttcg atctgaagaa aacccgacgg tggtgtgtac tggtgtggac
    12721 cgaggctgcg gtgttacgcg gtccgatgac cgaagacggg ttggcgtttg cccgccagct
    12781 agctaggaag aagcaccgct acttcgacaa caagctcgct ttggctgagc tgctgcagcg
    12841 gcgcaacctc agccagggac gtactcaagc tgaacggatt gcggcgttgc gggtctcccg
    12901 cgagcagtct tcgctggagc tcgatctcgc tgccgccatt gaggtttccc agcttccgac
    12961 cgtcatcaac gcgatcgagg agcaagccgc gatggacacg gtagccgagg aagctgcggc
    13021 attcggcgac gacctcgacg acactcccgc cgctatctac gacggattct acgacgatgt
    13081 gttggaggac gtatgagcac cgcaaccgcg cccacgcagg tggaggctaa gcgtcccaat
    13141 ttggacaatc tgaccttggc ccgcaaagaa ggctggcacg attacgttga gtcgccgaag
    13201 cgggtcagtc cagaactgct gtcaaagcgg gagattcgtc ggctcaacgg gccggcggct
    13261 gatatctaca accgacgccg tgctgattgg cacaacaatc tcgggccgtt caagaccccg
    13321 cagctcgccg ccgtgcacga cgcgttgtgg gacatcatgg acagcaacgc ccaggacgga
    13381 catcgcgcta agggtgctgt ggcgatcgac gggtaccctg ggctgggaaa gagttgtgct
    13441 gctgaagaat tcgccaaaca gttccatcaa cgcgagatcg tccgccacgg cgagttcacc
    13501 cgagccggcg acgaacggtg gcccgtgtgc cgaatcggga tgcggggcaa taccagcatg
    13561 aaggacttca acgccgccct gtgcgacttc tacgcccacc ccggccggcg cggcgccacc
    13621 gccgaacagc tgggcaggtg ggccctggac tgtgtgctga gttgtgaaac ccgcctactg
    13681 atcatcgatg acctccattt cctacattgg caacaccacg gtggcgtcga gatcagcaac
    13741 cacttcaaat acatcgccaa cgagttcccg ctcaccctgc tgtccatcgg cgtcgggctg
    13801 ggggagcgag gtgttctgtt ggagggcacc tcctacgacg acgcggtgct ggcccagacc
    13861 atgcgccgca caacgatgtt ggacatgcag ccgtatgcga tcactaccga gcgggcacgc
    13921 cggcaatggc gcacactttt ggtcaccatc gagcagcgac tgatcctcgg atcaaaacat
    13981 cccggtatgc tcgccgacgg gctgtcggac tacatgttca tccgaagcac cggccacatc
    14041 ggctcgctga tggaactcat cagacgagga tgcgcaaaag caatccgaac cggtgtggaa
    14101 accctcgacc agaagctatt cgacacaatc aaaatcgaca gcgccgccga gcgcgctcgc
    14161 cgcgaactag aagtggcatt tcgcacccgc cggctgacca cccgccggca gcttcggtga
    14221 cgcgcactct ccccgtcagg gccgcccccg tctctggcga ggcgctggac tcatggctgg
    14281 aaaccatcgc agcgcgttgt ggggccccgc tgggcgacat cctccgtgcc gtcggcataa
    14341 aaagccctat agcatgcggt cccggatggc ttctgcgcct ctccgacgaa gaacgacacc
    14401 agatcagctg cgcgacaggg attgacgaaa ccgcgatcgc agcgatgact ctcgcgcact
    14461 acaacggcac cgcactcgaa atcgactacg agcgccaccg cctgaaccat tcatttccct
    14521 tcggcgcccg ttcgtggtcg cgctactgtt cccaatgttt ggcggagaca cacggtcgct
    14581 ggcagctgca ctggcgcttg ggctggacgt tcgcatgtac ccgccactgc tgtctgatgg
    14641 ccgaccactg tccgaactgc ggccgccgac aacgcaagac ccacagccgg tacaacaccg
    14701 ttccgctacc cggccactgc agcggcaccg cgggatcatc ctgcggagaa aacctaacat
    14761 ccgcttcagt gttgcaactc cctacccacc acccggtgat cgaggcgcag aaaacactgt
    14821 tcgaaatgat cggggacgac cgcgcagcgt tcgggctgta cgccgcgcgg ccgtgctcgg
    14881 cccgcgaagc tctcactgac atcaaggcgc tggcgagccg gatcctcgcc tatgccgccg
    14941 acaatgggct gagggccatt gaacagtcca acctcttacc tcaatacggt gtcaaacggg
    15001 gcacggggga gggcgtgcac tggcgatacc ggcaggccac cacgatgatg aacgctccgc
    15061 agatcgccgt tgaagcagct gtggcgatca ccgcggcact gggcgttctg gaaagcgccg
    15121 acatcgcgct ggccgccgac aaagtccgtt ggctgattgc cgggcaactt caccgcggac
    15181 cgcccagcgc cctacagcga tacgctcgcg acggcgggct ggtcgctgcg gttctgctca
    15241 aggttttcgc gcctcctgtg gggtcgtcgc tgaccggttc gcatatgcaa ttgcgctatc
    15301 gcacaactct tcccgttccg acggcgccgg agtcagggaa aacgcggcta caccgaaccg
    15361 cacaccgact gccatcaatg ctctggccga actggtcgat ccgactacaa ctgcgcccct
    15421 ccagctactg gtatttacgc tgcctgctct cgtgtgccac gctgctcgtc ggcaccacgg
    15481 caagggtcga ggatgcgact gcagcgctgg gcggcgtgat cgacgccgac ggagccaaga
    15541 acgcgatatc gcagcttcaa caatccccac attgggcagc cgtctgctct gctgcaacgc
    15601 gactcagcga atacctcaac gacacaccag caccgatcga ctatcgccgc cgccgccgac
    15661 tcgactacac cgcgctgctc acccaggagc gctggatgca gatctgccgt caaaccgggg
    15721 ctctgccagg aaagagcgaa ggacaaaagg cgctcatcgc gcgcgcccat ctctaccaaa
    15781 aggtcagcgg ccgcccggca cacgaactcc tgcaggggac accaccggcg acgcgcgtag
    15841 acctcgtgca gcgcattgcc aacttcccgc gccgtatcac accagagttg gcgcgacttc
    15901 tcgatcagga atcgcggaac tttctgcgcg cgaaacacat cgacgaaccg atcacctgga
    15961 gcccgccact ggaattggtc aacgatctcg accttccaga acccgacccg gcacagacca
    16021 cgatcgacga cctgcacgct ctcgccgaag acatgacgat ttcgattacc gagatcgccg
    16081 gccgcctcaa caccagcgcg cacgcggtgc gctaccttct cgacaagcac ccgactcaac
    16141 cgcgaaaccg cagcgcggtg gaaagtgccc gtgcacacac gtgccccacc attaccgccg
    16201 acgtcctgca ggagctctac gttcagcaag ggctcacgat caatcagatc gcggcgcaat
    16261 tcggcacgac acgattcatg gtcaagcgtc ttctgcttcg tgatggcctg ccgatccgac
    16321 accggccacc cccacccagc ggtgactggc tctacgagca acacatcatc gagcgacgca
    16381 ccttgaccga catcgccatg gacaccggcg ttcaggcgcg cgcagtccac gtctggaaga
    16441 agcagcatca actcgcctcg cgaaccgaca aatcatcgtt ccgacgacgg ccgatgagcc
    16501 agggcaccgc caggcaggtc ttggaaccgt ggtacgccgc ccatgcagca gcatcctggc
    16561 tcaccaattt cactgagacc ctcagatatc cgaccatgca ggccgcggca gccgaactga
    16621 tcatcaaccc gtcgacactc cagtaccgga tcacggcgct ggaacgaatc ttcggagagc
    16681 cacttgtctt tcgcgcgcgc catggcctcg ccctgcgccc cactaacctc ggcgagcagg
    16741 tcgccgaggc catctcagct atcaaccccg aggcgacgct gtaaaccccc ggtacccggc
    16801 ctgccgtcgt cgaaacgatg cgcccgccac aagcagacac cagcgggcgc gtcggcgaaa
    16861 tcccgcgtcc ggatcaacat cgcttcgccc cagtcagcag tggacatgga tcgccgccat
    16921 gccaatcggc caaactctaa cacgaatgca ctactagtag tacatttgat aggtcggctt
    16981 ctggcactgg cgcccccaca cagccggctt agcagagtac tcgctacgtg gctgaacact
    17041 ctgccgcgct gcatatttca cgcagcactg tccccatagt cactgtaagc gcagcatcca
    17101 ggctcctgcg atgcgcgttg gcgagatgcg catgcccgca cgagcactat ccgaacgcga
    17161 tcggaccctg cctctgcgag tcgcacctat ccccggtgaa gcactcgact cttggctcga
    17221 ggccatcgcc tgtagatacc aacttccgat cggcgacgtg atgacacgct gcggcattgg
    17281 gcccaacgaa cggaccaccg cccggctggt gtcaccgacg agggacgaac gacgccagat
    17341 cagcaccgtg tgtggcatcg gcggcgacga aatccgatca atgacaatgg atcacttcga
    17401 caggcagcca gtcgatggcg accgcaggcc aacgtttctg tgggtacggc acgtcggatc
    17461 gcgtttctgt ccgcactgtc tgcgtgaagg cggtggacgc tggctattgg cctggcggct
    17521 caattggtcc ttcgcatgcc tcacacacca atgcctgctc gctgattcgt gttctgagtg
    17581 cgccagcatg caacgccgac aaccgttgtg ctcaagccga attccccgac ccagccgctg
    17641 cccctcggcc agcgagcgcg gccggacccg gggtgcggat cagtgcgggg cagacctcgg
    17701 tgccgccgac gtcttgcagt taccccaggc gcaccccgcg ctcaccgcgc aagcgacgat
    17761 tcaggcgttg ctcgacggga ctcggaccga gttggcgatc tacggctgcg tggcaccgac
    17821 ctgctcgagc atgctgggtg acctgcgtgt gctggcgcaa tgggtgatat acgccgtcaa
    17881 ccaggctcag ctcgacggcc acttaccgac cgacctgtcg agcgcggtgg cactgcaccg
    17941 ccaatccacc tcgtggccat atggccggca ctggcgcggc gcccgcaccg ttccatcggg
    18001 tctcgacacc gccgccggtg tatcactggc cctgggagtg atctccctac cgaacaccga
    18061 taccacggta tcgacgctgc gtcagctcat gcacgacgca cacaacggcg gaccgtacca
    18121 aacgcccatg tcgcgccgag ccgctctcag cccagccctc acgtccgttc tcgaggccgc
    18181 catcgtcgca cacgtagccg accgcaaact gcggagccgc gttgcgcgca agctcgccag
    18241 cttgtcgtcg gttccagctg ggtcgacccc cttgcctcgt tagaccttgc gtggccaagc
    18301 gtgacggatg atgagccgat gggcaaagac caacccacgc acctggaccc acaccagctg
    18361 gccgccctgg cggccgtggt tgaggagggc agcttcgacg cggcagcaga gacgctgcac
    18421 gtgacacctt cggcggtcag ccagcggatc aaaactctcg aacagcacat cgggcaagtc
    18481 ttggtcgtgc gtcaaaaacc ctgccgaccc accgctgccg gtgtaccgct gttgcgattg
    18541 gccgcccaga tggccctcct cgaggcagag gtgctcgccg aatccgcagg ccgatcagca
    18601 caactgaccc gaatcgcgat cgcggtcaac gccgactcaa tggcgacctg gttcaccgcg
    18661 gtccttggca cactgtccga cgtcctcttc gacatccgca tcgaggacca ggaccactcc
    18721 gctcggctgc tgcgcgaagg cgccgtcatg ggagcggtca ccaccgaacg cagtgctgtc
    18781 ccggggtgtc gcgtgaagtc actgggcgtg atgcgctacg taccggtggc cagcgctgcc
    18841 tacgtggaac ggtacctttc ggacgggttc acccgagagt cggtcaaagc tgccccatcg
    18901 ctcgcttgga accgtgacga tgcgctgcag gacatgttgg taagaaaaat attccggcgc
    18961 gccatcgcac gcccgatcca ttacgtaccc accgccgaag gcttttcggc ggccgtgcgt
    19021 gccggtttgg ggtggggaat gtttcccgaa aagctagccg cccgcgacct gactcagggc
    19081 gtattcgtcc acgtcgccga cacctatctt gatgtgccgt tgtactggca gtgctggaaa
    19141 atggacagtc cccttatcga aaccatcacc gacgcggtcc agtcagccgc ccaggtgctg
    19201 caccgacacc gaacgaaacc gcgaacctca agccccaacc gctcttgatg ccgataccac
    19261 catcagcaac acggccgtcg ttacacccgc gaatcaacgg ccgcacacgg ccgggcggcc
    19321 ggcgactcga tgtatcgcct ccgtccagcc tgataacgag cgcggctagt catctgcgac
    19381 caggagctcg gccgcgggta catcaagtgc gtcggcaagg tcgaacagcc gctcgtagag
    19441 caggctgcgt cggccgtgtt cgacatcgat gagcacattg cgcgtcacac cggacagcag
    19501 ggcaagggcc tcctgggtca tccctcgctc ggtacgaagg gctcgtatcc gctggccgac
    19561 aacgcgacgt cgctgctccc acagtgccac ccgctcggga ctgttttggc tggggcgcgt
    19621 cggcacgcaa cctagtaaca gctgcgcccc ctcaactgtc tgccctacta gtagtactat
    19681 gcgtgagggt tccagcgagt tctctaacat gcgccctgga cactcacctc ggcctgcccc
    19741 gagcccaccc gcctcgcctg gcgaaccgtc cgcttcggac gatcggaact gcgggggacg
    19801 gaaaggacgc gtctagcaaa aatggatgag ctgccctcat cactgcggga gtacctcgac
    19861 ctctggtgcg ctgatctgct tgatccccta actgcggcgc ggcgtgatca ggtgtgcagc
    19921 ctcgttgagt ccagctatgc gggcgggccg tggccaccgc gattgctggt ccagcgaatg
    19981 tgtgagctca acactggctg catcgacata gatgcgttcg tcggcgatgt aaccacacga
    20041 tgggggtgca gcatcgatga cgccgtgagg atgctgtctg ctgaggactg tgatgatcgc
    20101 gccaatgcga tagtcctgtt gggacgctat cccgacacca ctgagctcgt cgagaaagtg
    20161 actgcggcgc acca
//
LOCUS       DASRJA010000058        14369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2488380,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000058 DASRJA010000000
VERSION     DASRJA010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14369
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2488380"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..2016
                     /gene="treS"
                     /locus_tag="VFC01_02530"
     CDS             250..2016
                     /gene="treS"
                     /locus_tag="VFC01_02530"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HZN78552.1"
                     /translation="MDPSSAVHDPAEGSHVEDGVVEHPTSEDFGHARTLPADRTWFKR
                     AVFYEVLVRAFYDSNSDGAGDLRGLCEQLDYVKWLGVDCLWLPPFYDSPLRDGGYDIR
                     DFYRVLPEFGTVDDFVDLLDAAHRRGIRVITDLVMNHTSDSHEWFQESRRNPDGPYGD
                     YYVWSDTSDKYSDARIIFVDTEESNWTFDPVRRQFYWHRFFSHQPDLNYDNPAVQNAM
                     LDVLRFWLDLGIDGFRLDAVPYLFEREGTNCENLPETHSFLKHCRKVIDDEFPGRVLL
                     AEANQWPADVVEYFGDPSTGGDECHMAFHFPLMPRIFMAVRRESRFPISEILANTPDI
                     PDMAQWGIFLRNHDELTLEMVTDEERDYMYAEYAKDPRMKANVGIRRRLAPLLENDRN
                     QIELFTALLLSLPGSPVLYYGDEIGMGDIIWLGDRDGVRTPMQWTPDRNAGFSTATPG
                     RLYLPPNQDAIYGYQAVNVEAQRDSSNSLLNWTRTMLAVRGRHDAFAIGSFRELGGSN
                     PSVLTFVRESDGDIVLCVNNLSRFPQPIEMNLQNWNGYTPVEMTGHVEFPRIGQLSYL
                     LTLPGHGFYWFQLRAPEPGEQS"
     gene            2013..3365
                     /locus_tag="VFC01_02535"
     CDS             2013..3365
                     /locus_tag="VFC01_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltokinase"
                     /protein_id="HZN78553.1"
                     /translation="MNLPFDEWIVHQRWYAGRNRELASAQPAVVVPLRDDLDLVVLDV
                     AYTEGASQRYQVIVRWDSGAADAATSGGSPISEYLSVATIGSDEDRTAYDALYNPADA
                     KYLLSLIDSSATVGDLRFAKEPDVTLPVDAAPRVSSAEQSNTSVIFEEQAVFKVFRRI
                     TPGINPDIELNRVLARADNEHVARLLGSYETTLGDEPYALGMVTEFAANSAEGWDMAT
                     ASTRDLYAEGDLYADEVGGDFAGESYRLGEAVASVHATLAEELGTSTVLFPADIMLER
                     LSAAAAAVPQLEQYVSLIEERYQKLAEEPITVQRIHGDLHLGQVLRTPERWLLIDFEG
                     EPGQPLDERRRPDSTLRDVAGVLRSFEYAAYQRLMDQGGDEDRERQLAARAREWVNRN
                     AASFCDGYAVVCEADPRDSRHVLEAYELDKAVYEAAYEARHRPSWLSIPLKSIARIVG
                     "
     gene            3449..4804
                     /locus_tag="VFC01_02540"
     CDS             3449..4804
                     /locus_tag="VFC01_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN78554.1"
                     /translation="MDFCVVGAGFAGLTAALRLKQAGHSVALLEARDRVGGRTFTVTR
                     DDGSWIDRGGAWVGPGQDRIHALMNEYGVPSYKQYIDGKAMMVIDGKQHRYSGTIPWT
                     LSPWASANLGAVFLELGQMSKSIPLEAPWEAKNAEKWDQITLAKWLSSNTISKAAHDL
                     LETALAGCYTSAASEVSMLFVLYQMASGGGPGFLLGIKDGAQDSRIVGGMGAVYGPMA
                     AEIGDALHLSQPVRRIDQDIDGVTVRSDDMVVRAQRVIVAVPLAIASQIIYEPMLPVD
                     RSFLHQRMPSGAIYKINIVYDEPFWRNDGLSGQSAAPGSPATVTIDASTDAPRPGVLC
                     VIVEGPIARQIGRLDADDRRRTILAELVGRFGDKAASPVDYLEQCWTTERYSGGGMLS
                     HAPTGVLTEFGPALREPCGRIHWAGTESSAIMCGWVDGAVRSGERAAAEVMQREMVGM
                     A"
     gene            4836..5345
                     /locus_tag="VFC01_02545"
     CDS             4836..5345
                     /locus_tag="VFC01_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HZN78555.1"
                     /translation="MTGYQINSIRQNTIDTVAGVFARYGLVIVIAWFGGMKFTNYEAQ
                     GIQPLVSESPFMSWLYEIFSVYTFSVLLGVFEVAAAVLIAVKPWWPRVSILGSALAIL
                     LFAATISFLFTTPGVFEESVGGFPLLSLTGGFLIKDVALLGISVWTLADALRATRSSL
                     AGSSQAHAT"
     gene            5428..7275
                     /locus_tag="VFC01_02550"
     CDS             5428..7275
                     /locus_tag="VFC01_02550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN78556.1"
                     /translation="MAAMTWPRVSLILLLVVTAVMYLWNITINGMGNQFYAAAAQAGS
                     KNWEALLFGSLDPQNFITVDKPPVSQWVMGLSGQIFGFNSASMLIPEALMAVASVALV
                     YCAVTRISGPRTGLVAGAVLALTPVAALMFRFNNPDAVMVLLMTAAAYCTVRAVQQAS
                     PKWLAWAAVALGFAFLAKMLEGLMVAPALAAAYLIAAPTTLRTRLRHIAVAAVAFVVS
                     AGWFVLLTMLWPASSRPYIAGSADNNFMNLVLGYNGFARVLGRNHVGFGPPDQVVGSA
                     AGARLNPGGHGGFGGFGNQSQGLSRLFSGEFGFEIGWLVPAALLATVLVVLSRGRAPR
                     TDLTRAAAILFGGWLIIDGLVLSFMHGAIHAYYCLSIAPPVAAMFAIGVHELWRNRET
                     WWYRIGLAILIGGTGVWSWLILGRNVAWLPGLRWTILAAAVVAAVALAVPGTYQVRRR
                     LAFGVMGVGLVAALAGPAAYAIVTIGQPHQGGGPSVGPADASRQGHFGFGQSADNPEL
                     DAMLKNSNTEWSAAISRSSSAAGLELSTDTAVMAIGGFSGSDPAPTLSQFQDDVAQHK
                     VAYYIVVNNRGHGPGWTNRGHADVAKWVAATFPSTNVGDATVYNLSAPK"
     gene            complement(7276..8028)
                     /locus_tag="VFC01_02555"
     CDS             complement(7276..8028)
                     /locus_tag="VFC01_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN78557.1"
                     /translation="MAMRVGAFAVVELQKLRHDRTELFTRMVQPALWLLIFGQTFSRL
                     HVIDTGQVPYLAFLAPGIIAQSALFISIFYGIQIIWDRDAGILAKLMVTPAPASAIIT
                     GKAFAAGVRSVVQVVGVVALAFLLGVHLTVNPLRILGAMGVVVLGAAFFACLSMTLAG
                     LVRNRDRLMGIGQAITMPLFFASNALYPVDVMPQWLRWLSAVNPLSYEVNALRGLLIG
                     TPTNGLLDIAVLVLAAVVGIAMASALLRRLVR"
     gene            complement(8087..8893)
                     /locus_tag="VFC01_02560"
     CDS             complement(8087..8893)
                     /locus_tag="VFC01_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN78558.1"
                     /translation="MTLAIDCQHLTHRYGHFTAVDDLSLQVHCGETVGLLGPNGAGKT
                     TVVRVLTTLTPVQSGHVSVFGLDSHRHTMDIRHNIGYVPQQLSIEAALTGRQNVELFA
                     RLYDVPRGQRSERVDEALHAMQLHDVADVVAGTYSGGMVRRLELAQALVNRPSLLILD
                     EPTVGLDPIARDSVWAQVGRMQREFGMTVLLTTHYMQEADALCDRVALMHHGRLQAVG
                     SPAELKPKVSPDATLEDVFRHYAGSDLGEDTSQTGLRAVRNTRRTARRVS"
     gene            complement(8890..9351)
                     /locus_tag="VFC01_02565"
     CDS             complement(8890..9351)
                     /locus_tag="VFC01_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN78559.1"
                     /translation="MGGVDTRTDLTAELFNVVGRFRRQLRRSTGHGFDATGLTQSQAE
                     LLRLVARQPGISVREAATELGLAPNTASTLVSKLSTDGLLIRTVDADDRRVGRLRLTE
                     PAQRIADESRAARRAALSAVLDELEPGQLVRLERGLEVLAEMTRMLHERQP"
     gene            9434..10060
                     /locus_tag="VFC01_02570"
     CDS             9434..10060
                     /locus_tag="VFC01_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS and ANTAR domain-containing protein"
                     /protein_id="HZN78560.1"
                     /translation="MDGRGWPPAGSFKYHARDDRWEWSDEVAAMHGYDPGTVTPTTEL
                     VLSHKHPDDKPTVAQLIEQVRQFGIPFSSRHRIIDTAGKVHVVVVVGDRWKDDAGEVI
                     GTTGFYVDVTDEFDADVRRSLDEVVATIAARRATINQAMGMLMLGHGISAEKAFAMLA
                     QRSQQTNVKLRDLAARFVEEAAKIPLPNAARLDDILANAHEGLGEPRQ"
     gene            10065..10436
                     /locus_tag="VFC01_02575"
     CDS             10065..10436
                     /locus_tag="VFC01_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78561.1"
                     /translation="MASYLLNEAGVAKARTLIDARQYVLDSDWGEVQPKADAQNSYLD
                     SHSWDEYAEWHLGLTEGATDETKARYAFVYGDFRRIHRTGLIACVYRASEWRHKDVEL
                     AAHDLLQYLDKKTGLEPTTRA"
     gene            complement(10441..11001)
                     /locus_tag="VFC01_02580"
     CDS             complement(10441..11001)
                     /locus_tag="VFC01_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78562.1"
                     /translation="MAPRRGSPRRLTAEDWIEAGFAVLAEGGPNALRVDSLCERLNVT
                     KGSFYWHFTDMPAYRGALIKAWSGLHDRDRQHFETMPDVDPRERLTVMIQTWVAPRHW
                     QLERAMRVWALTDDEVLGSVQQSDARVLRAVRQAFVDVGFGREEAALRSYVVFALGIG
                     SLHSSGSAPATAPDLQDRFLDFMLRP"
     gene            11313..11666
                     /locus_tag="VFC01_02585"
     CDS             11313..11666
                     /locus_tag="VFC01_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78563.1"
                     /translation="MARDDFDDNPLEPAEELDSDEVRNADGDEVVDPPDEWIEAKDNE
                     TLDERLAEEVPDDQGDDEADDARSDAHDSDAGGGLRLVSNYDIDHMDPQTHGRERGQI
                     DGTPEDGDSFYDVIE"
     gene            11717..12019
                     /locus_tag="VFC01_02590"
     CDS             11717..12019
                     /locus_tag="VFC01_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78564.1"
                     /translation="MDSDDTLRPAEALDSDDVRNEDGDEVVDPPEGWAAADHIAEEPE
                     GESLDEKLAAEEPDASFPAPDEAEDVDHVDPAHHGTDEGQISGSPEDGDSIFPVVE"
     gene            complement(12021..14156)
                     /gene="glgX"
                     /locus_tag="VFC01_02595"
     CDS             complement(12021..14156)
                     /gene="glgX"
                     /locus_tag="VFC01_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HZN78565.1"
                     /translation="MRPELEVWPGRSYPLGATYDGAGTNFSLFSEVAEKVDVCLFDAD
                     GSEQRVALEERDAFCWHAYLPTVGFGQRYGYRVHGRWAPEEGVRCNPAKLLLDPYAKA
                     IEGQVDWRPACFPYEFGDDSKRNDEDSAPHVPKSVVSVPFFDWRNDRPPDIPLHESVF
                     YEVHVKGFTARHPGIPRHLRGTYAGLAHPVAIDYFRSLGVTAVELLPIHQFVHDAQLV
                     ERGLRNYWGYNSIGFFAPHNEYASSGQTGEQVQEFKGMVRALHDAGIEVILDVVYNHT
                     AEGNHLGPMLSFKGIDNAAYYRLVPEDRGRYYDTTGTGNSLNMRHPHALQLIMDSLRY
                     WVREMHVDGFRFDLAATLARQFHEVDRLSAFFDLVQQDPIVSQVKLIAEPWDVGEGGY
                     QVGNFPPLWSEWNGKYRDTVRDYWRGEPATLDEFAYRLTGSADLYEQTGRRPVASINF
                     VIAHDGFTLRDLVSYNEKHNEANKEGNRDGESHNRSWNCGAEGPTKDPEINALRARQQ
                     RNFLATLLLSQGVPMIAHGDELGRTQQGNNNVYCQDNETSWIDWANADAELMEFTRSV
                     SALRTAHPVFRRRRFFSGKPVGRRGDGGQPDIAWFAPDGSQMTGDDWETGYAKSIAVY
                     LNGQGIPGRDVRGQRVTDDSFVLCFNAHYEPIEFSLPPKEFGSAWVPVIYTADTATAE
                     EAKPVTAGAKVAVDARAVLVLQASDQTPG"
     gene            complement(14153..>14369)
                     /locus_tag="VFC01_02600"
     CDS             complement(14153..>14369)
                     /locus_tag="VFC01_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZN78566.1"
                     /translation="ILAAYRTVARAPSRLVAVTLEDAVGEVERPNVPGADGRRPNWSL
                     ALRAPLETLEAEPLPRELAAVMREEEP"
BASE COUNT         2452 a         4722 c         4760 g         2435 t
ORIGIN      
        1 atcattaggc gtcgaatcag ccacgaccac tcgattcttt cggacaaaca acgccagtaa
       61 tggccagatc gccacgctga cggatctagg acgtcgaaac cgtaacccga ctcgattgcg
      121 gattcaaccg gtcgtcgacg agcttcggtg aatacgaggt tgccagccgc ccgccacttg
      181 gttgcggacc ggtcagtgcc aattcctcgg cgattcagtt ttggccgact tgggtaagtt
      241 tgaaggctga tggacccaag cagcgcggta cacgatccgg cggaaggcag ccacgtcgag
      301 gatggcgtgg tcgaacatcc aacctcagaa gacttcgggc acgcccgcac cctgccggcg
      361 gaccgcacct ggttcaaacg tgcggtgttc tacgaggtcc tggtgcgggc gttctacgac
      421 tccaactctg acggcgcggg tgatctgcgc ggcctgtgcg aacagctcga ctacgtgaag
      481 tggcttggcg tcgactgtct ttggctgccg ccgttctacg actcgccgct gcgtgacggc
      541 ggctacgaca tccgcgactt ctaccgggtg ctgccggagt tcggcaccgt cgacgatttc
      601 gtcgacctgc tcgacgccgc gcaccgccgc ggcattcgcg tcatcaccga tctcgtcatg
      661 aaccacacgt ccgattcgca tgagtggttc caggaatcga ggcgaaatcc cgacgggccg
      721 tacggcgact actacgtgtg gagcgacacc agcgataagt actccgacgc gcgaatcatc
      781 ttcgtcgaca ccgaggagtc gaactggacg ttcgacccgg tgcgacgcca gttctactgg
      841 caccggtttt tctcgcatca gcctgatctc aactacgaca acccggccgt gcagaacgcg
      901 atgctcgacg tgcttcggtt ctggctcgac ctcggcatcg acggtttccg gctggacgcc
      961 gtgccctacc tgttcgagcg ggaaggcacc aactgcgaga acctgcccga gacgcattcg
     1021 ttcctcaagc actgccgcaa ggtgatcgac gacgagttcc ccggccgggt gttgcttgcc
     1081 gaggcgaatc agtggccggc cgacgtggtc gagtacttcg gcgacccgag caccggcggc
     1141 gacgaatgcc acatggcgtt ccacttcccg ctgatgccgc gcattttcat ggcggtgcgc
     1201 cgcgagtcgc gattcccgat ctcggagatc ctcgccaaca ccccggacat cccggacatg
     1261 gcgcagtggg gcatcttcct gcgcaaccac gatgagctga cgctcgagat ggtcaccgac
     1321 gaagagcgcg actacatgta cgccgagtac gccaaggacc cgcggatgaa ggccaacgtc
     1381 ggcatccgcc gtcggctcgc gccgctgctg gagaacgatc gcaaccagat cgagttgttc
     1441 accgcgctgc tgttgtcgct gcccggctcc ccggtgctct actacggcga cgagatcggg
     1501 atgggcgaca tcatctggct cggcgaccgc gacggggtgc gcacgccgat gcagtggacg
     1561 cccgaccgca acgccgggtt ctcgaccgcg acgcccggcc ggctgtacct gccgccgaac
     1621 caggacgcca tctatggcta tcaggccgtc aacgtcgagg ctcaacgcga cagttcgaac
     1681 tcactgctga actggaccag gaccatgctt gcggtgcgcg gtcgccacga cgcgttcgcg
     1741 atcgggtcct tccgcgaatt gggtggctcc aacccgtcgg tgctcacctt cgtccgggag
     1801 tccgacggcg acatcgtgct gtgcgtcaac aacctgtcgc ggttccccca gcccatcgag
     1861 atgaacctgc agaactggaa cgggtacaca cccgtagaga tgaccggaca cgtcgaattt
     1921 ccccgcatcg gacagctgtc ctacctgctg acgctgcccg gacacggttt ctactggttc
     1981 cagctgcgcg cgccggagcc tggagagcaa tcatgaacct gccgttcgac gaatggatcg
     2041 tgcaccaacg gtggtacgcc ggtcgcaacc gtgagctggc atcggcacag cccgctgtcg
     2101 tggtcccgct gcgcgacgac ctcgacctcg tggtgctcga cgtcgcctac accgagggcg
     2161 cctcgcagcg gtatcaggtg atcgtgcgct gggactccgg agccgcggat gcggcgacat
     2221 ccggcggaag cccgatttcc gaatatctct ccgttgccac tatcggctcc gacgaggacc
     2281 gcaccgcgta cgacgcgctg tacaacccgg cggacgcgaa atacctactg tcgctgattg
     2341 attcgtcggc aacggtcggc gacctgcgtt tcgccaagga gcccgacgtc acgctgccgg
     2401 tggacgccgc gccgcgggtg tcgagcgccg aacaatccaa caccagcgtg atcttcgaag
     2461 aacaggccgt gttcaaggtg ttccggcgca tcacgccggg catcaatccg gacatcgaat
     2521 tgaaccgggt gctggcccgg gcagacaacg agcatgtggc gcggctgctt ggttcgtacg
     2581 aaacgacgct cggcgacgag ccgtacgcgc tcggcatggt caccgagttc gcggcgaatt
     2641 ccgccgaggg ctgggacatg gccacggcca gcacccgcga cctctacgcc gagggtgacc
     2701 tctacgccga cgaggtcggc ggcgacttcg cgggcgagtc ctaccggctc ggcgaggccg
     2761 tcgcatccgt gcacgccacc ctcgccgaag agctcggcac gtcgaccgtt ctgttcccgg
     2821 ccgacatcat gctggagcga ctgtcggcgg cggccgcggc ggttcctcaa ctcgagcagt
     2881 acgtttcgct gatcgaggag cggtaccaga agctggccga ggagccgatc accgtccaac
     2941 gcattcacgg tgacctgcac cttggccagg tgttgcggac tcccgagcgc tggctgctga
     3001 tcgacttcga gggcgaaccc ggacagcctc tcgacgagcg ccgccggccc gactccacgt
     3061 tgcgtgatgt ggcgggcgtg ctgcggtcgt tcgagtatgc cgcctatcaa cgcttgatgg
     3121 atcagggcgg cgacgaggac cgggagcgcc agctcgccgc tcgcgcccgc gaatgggtga
     3181 atcgcaacgc agcgtcgttc tgcgacggct acgcggtggt ctgtgaggcc gacccacgcg
     3241 actcccggca tgtgctcgag gcctacgaac tcgacaaggc cgtctacgag gccgcgtacg
     3301 aggcacggca tcggccgagc tggctgtcga tcccgctgaa gtcgatcgcg cggatcgtcg
     3361 gctgaacggt ttttgacgtc gccgtaaaca aatttgccaa aacgcttcgg tgggccggaa
     3421 tgtagcggac catcctgtcg tggttgacat ggacttttgc gtggttgggg cgggtttcgc
     3481 tggattgacg gccgctttgc ggctcaaaca ggctgggcac tcggtggcgc tgctggaggc
     3541 ccgcgaccgg gtcggcggcc gaacgttcac cgtcactcgg gacgacgggt cctggatcga
     3601 ccgcggcggc gcctgggtcg gcccggggca ggaccgcatc catgcgctca tgaacgaata
     3661 cggcgtgccg agctacaagc aatacatcga cggcaaggcc atgatggtca tcgacggcaa
     3721 gcagcaccgc tactcgggca cgatcccgtg gacgttgagt ccgtgggcgt cggcgaacct
     3781 cggcgctgtc ttcctcgagc tcggacagat gtccaaatcc attccgctcg aggcgccgtg
     3841 ggaagcgaag aacgccgaga agtgggacca gatcactttg gcgaagtggc tcagctccaa
     3901 cacgatctcc aaggccgcgc acgatctgct cgagaccgcc ctggccggct gctacacctc
     3961 cgccgcatcg gaggtctcga tgctgttcgt gctctaccag atggcatcgg gcggcggccc
     4021 gggtttcctg ctcgggatca aggacggcgc gcaggactcc cggatcgtgg gcggtatggg
     4081 cgccgtctat gggccgatgg ccgccgagat cggcgacgcg ctgcacctgt cacagccggt
     4141 acgacgcatc gaccaggaca tcgacggcgt cacggtccgc tccgacgaca tggtggtgcg
     4201 ggcgcaacgc gtgatcgtcg cggttccgct cgcgatcgca agccagatca tctacgaacc
     4261 catgctgccg gtggaccggt cgttcctgca ccagcgcatg ccgagcgggg cgatctacaa
     4321 gatcaacatc gtctacgacg agccgttctg gcgtaatgat gggctctccg ggcagtctgc
     4381 ggcccccggt tcgcccgcca cggtcaccat cgatgcgtcg acggatgcgc cgcgccccgg
     4441 cgtcctgtgc gtgatcgtcg aaggcccgat cgctcggcag atcggccggc ttgacgcgga
     4501 cgaccgcagg cgaaccatcc tcgccgaact cgtgggacgg ttcggggaca aggccgcatc
     4561 gccggtcgac tatctggagc aatgctggac caccgaacgc tactccggcg ggggcatgct
     4621 cagtcacgcc ccgacgggcg tgctcaccga gttcgggccg gcgctgcgcg aaccgtgcgg
     4681 gcggatccac tgggccggaa ccgagagttc ggcgatcatg tgcggctggg tcgacggcgc
     4741 ggtgcgctcc ggtgagcgcg cggcggccga agtcatgcaa cgcgaaatgg tcggcatggc
     4801 gtaacggtcg ccggcggtcg cggcactacg gctacatgac cggttaccaa atcaactcca
     4861 tcagacagaa cacaatcgat accgtcgccg gcgtgttcgc gcgctacggc ctggtcatcg
     4921 tgatcgcgtg gttcggtgga atgaagttca cgaactacga ggcccagggg atccagccgc
     4981 tggtctccga aagtccgttc atgagctggc tgtacgagat cttctcggtg tatacattct
     5041 ccgttctgct cggcgtgttc gaggtcgccg cagcggtttt gatcgccgtc aagccgtggt
     5101 ggcccagggt gtcgatcctc ggcagcgcgc tcgccatcct gctgttcgcc gccacgatca
     5161 gcttcctgtt caccaccccc ggcgtcttcg aagaatcggt cggcggcttt ccgctcctgt
     5221 cgttgaccgg cggctttctg atcaaggacg tcgcgctgct tggcatttcg gtgtggacgc
     5281 tggccgacgc gctgcgggca acccgcagca gtctcgccgg ttcctcacag gctcacgcaa
     5341 cctagcgatg actcactggc tggtgtgaca tcgacgttag agacgccggc gccgcccgcc
     5401 gataccgaga ccgcaccgcc caccccattg gccgcgatga cgtggccccg ggtgagcctc
     5461 atcttgctct tggtcgtcac cgcggtgatg tatctgtgga acatcaccat caacgggatg
     5521 ggcaaccagt tctatgccgc tgcggcgcaa gcgggttcga agaactggga agcgttgttg
     5581 tttggctcgc tggatccgca gaacttcatc accgtcgata agccgcccgt ctctcagtgg
     5641 gtgatggggc tgtcggggca gatattcggt ttcaacagtg ccagcatgct gatcccggag
     5701 gccttgatgg ccgtcgcgtc ggtcgccctg gtgtactgcg cggtgacccg catcagcgga
     5761 cccaggaccg ggctggtcgc cggcgcggta ctggcgttga caccggtggc cgcgttgatg
     5821 tttcggttca acaatcccga tgccgtgatg gtgctgctga tgacggccgc ggcgtactgc
     5881 accgtgcgtg ccgtgcagca ggcgagcccg aagtggctgg cgtgggccgc tgtggcactg
     5941 ggtttcgcgt tcctggccaa gatgctcgag ggactgatgg tggccccggc attggccgct
     6001 gcgtacctca tcgccgcgcc gacaacgctg cgcacccggc tgcgacacat cgccgtggcc
     6061 gcggttgcct tcgtcgtctc ggcgggctgg ttcgtcctgc tgaccatgct gtggccggcg
     6121 tcgtcgcgtc catacatcgc cggctcggcc gacaacaatt tcatgaattt ggtgttgggc
     6181 tacaacggtt tcgcccgtgt gctcggccgc aatcacgtgg gcttcggccc gccggaccag
     6241 gttgtcggat cggcggctgg tgcgcgattg aaccccggag ggcacggcgg gttcggcggg
     6301 ttcggcaatc aatcgcaggg cttgtcgcgg ctgttctccg gcgaattcgg ttttgagatc
     6361 ggctggctgg tgcctgcggc gctgctagcc acggttctgg tcgtgctgtc tcgcggacgt
     6421 gcaccccgga cggacttgac gcgggcagcg gcaatcctgt tcggcggctg gctgatcatc
     6481 gacgggctgg tgctgagctt catgcacggc gcgatccacg cgtactactg tctgtccatt
     6541 gcaccgccgg tggcggcgat gttcgcgatc ggtgtgcacg aattgtggcg caaccgtgaa
     6601 acatggtggt accggatcgg tttggcgatc ctgattggcg gcacgggtgt ctggagctgg
     6661 ttgatcctcg gccggaatgt agcctggctg cccggcctgc ggtggaccat tctggcggcg
     6721 gcggtggtag cggcggtcgc tttggcggtc ccagggacgt atcaggtgcg ccgcaggctc
     6781 gcattcggag tcatgggtgt gggactggtg gctgcgttgg cgggaccggc cgcctatgcg
     6841 atcgtcacga tcggtcagcc gcaccaaggt ggcggaccgt cggtcgggcc ggccgacgcg
     6901 tcgcggcagg gccacttcgg cttcggccaa agtgcggaca acccggagct cgatgcgatg
     6961 ctgaagaaca gcaacaccga atggtcggcc gcgatcagcc ggtcatcgag cgcagcgggc
     7021 ctcgaattgt caacggacac agcggtcatg gcgatcggcg gcttttcggg cagcgatccg
     7081 gcgccgaccc tgagccagtt ccaggacgat gtggcgcagc acaaggtggc ctactacatc
     7141 gtcgtgaaca accgtggcca cgggccgggc tggacgaacc gcggtcacgc cgatgttgcg
     7201 aagtgggtgg cggccacctt cccgtcgacg aacgtcggcg atgcgacggt gtataacctg
     7261 tctgcgccga agtagctagc gcacaaggcg tctcagcaac gccgacgcca tcgcgatgcc
     7321 cacgaccgcg gcaagcacga gcacggcgat gtcgaggagg ccgttggtcg gcgtgccgat
     7381 cagcagcccg cgcagcgcgt tgacttcata gctgagcgga ttgacggccg acagccagcg
     7441 cagccactgc ggcatcacgt cgaccggata cagcgcgttc gacgcgaaga acagcggcat
     7501 cgtgatggcc tgcccgatgc ccatcagccg gtcgcggttg cgcaccaggc cggccagcgt
     7561 catcgacaga cacgcgaaga acgccgcgcc cagcaccaca acgcccatcg ccccaaggat
     7621 tctcagcggg ttgacggtca ggtgcacgcc cagcaggaac gccagcgcca ccaccccgac
     7681 cacctgcacc accgaacgca cgccggccgc gaacgccttc ccggtaatga tcgccgacgc
     7741 tggcgcgggg gtcaccatca actttgcgag gattcccgcg tcgcggtccc aaatgatctg
     7801 gatgccatag aagatcgaga tgaacagcgc cgactgcgcg atgatgcccg gcgccaagaa
     7861 cgccaggtat ggcacctgcc cggtgtcgat gacgtgcagc cgggagaacg tctgcccgaa
     7921 gatcaacagc cacagcgcgg gctgcaccat cctggtgaac agctcggtgc ggtcgtggcg
     7981 cagcttctgc agctccacca ccgcgaaagc gcccacccgc atggccattc cacggatccg
     8041 gcgccacccg cgcggcgccc gaacgatctc gacttccacc ctctgctcaa ctgacacggc
     8101 gcgccgtcct cctggtgttc cggaccgcac ggagcccggt ctgcgacgtg tcctcgccga
     8161 gatccgagcc ggcgtagtgg cggaagacgt cctcgagtgt cgcgtcaggc gatacttttg
     8221 gcttcagttc cgccggcgag ccgaccgctt gcagccggcc gtggtgcatc agcgcgaccc
     8281 gatcgcacaa cgcgtcggcc tcctgcatgt agtgggtcgt gagcaggaca gtcatgccga
     8341 actcgcgctg catgcgtccc acctgggccc agacactgtc gcgcgcaata ggatccagac
     8401 cgacggtcgg ctcgtcaagg atcagcagcg acggccggtt gaccaacgcc tgggccaact
     8461 cgagccggcg aaccataccc ccggaatatg taccggccac cacgtcggcg acatcatgca
     8521 actgcatggc atgcagcgcc tcgtcgacgc gttcggaccg ctggccccgt ggcacgtcgt
     8581 aaagccgcgc gaacagctcg acgttctgcc ggccggtcaa tgcggcctcg atcgaaagct
     8641 gttgcggcac atagccgatg ttgtgccgga tatccatcgt gtgccgatgt gagtcgaggc
     8701 cgaagaccga aacgtgacca ctttgtacgg gcgtcagcgt ggtcagcacg cgcaccaccg
     8761 tggtcttgcc ggccccgttg gggccgagca ggccgaccgt ttcgccgcag tgcacctgca
     8821 gggacagatc gtcgaccgcg gtgaagtgac cgtagcggtg ggtgaggtgc tgacagtcga
     8881 tcgcaagtgt catggttgcc tctcgtgcag catgcgggtc atctcggcga gtacctccaa
     8941 gccgcgctcc agtcggacga gctggccggg ctccagctcg tcgagcaccg ctgacagggc
     9001 ggcgcggcgc gcggcgcgcg attcgtcggc gatgcgttgt gcaggttcgg tcagccgcag
     9061 ccggcccacc ctgcggtcgt cggcgtcgac ggtgcggatc agcaggccgt cagtcgagag
     9121 tttggacacc agcgtcgatg ccgtgttcgg cgccaagccc aactccgtgg ccgcctcccg
     9181 cacggagata cccggttggc gggcgaccaa gcgcagcaat tccgcctgcg actgcgtcaa
     9241 cccggtggcg tcgaagccgt ggccggtcga tcggcgcagt tggcgccgga accggccgac
     9301 cacgttgaag agctcggcgg tcagatcggt gcgggtgtcc accccgccga tattacctct
     9361 gtttcagagc cattttctca ccgcggaggc ttcggcggga catactccaa taggacgtct
     9421 tagggggtgc accatcgacg gacggggctg gccgccggcg ggcagtttca agtatcacgc
     9481 tcgtgacgat cgctgggagt ggtccgacga ggtggcggcg atgcacggct atgacccggg
     9541 aaccgtcacc ccgaccaccg aattggtgct gtcgcacaaa caccccgacg acaagccgac
     9601 cgtggcccag ttgatcgaac aggtgcgcca gttcggtatt ccgttttcca gcaggcaccg
     9661 catcatcgac acagcgggca aggttcacgt ggtggtcgtg gtcggggacc ggtggaagga
     9721 cgatgccggt gaggtcatcg gcaccacggg cttctacgtc gacgtcaccg acgagttcga
     9781 tgccgacgtg cggcgctcac tggacgaggt cgtcgccacc atcgccgcgc gccgggccac
     9841 gatcaaccag gcgatgggca tgctgatgtt gggccacggc atctcagccg agaaggcctt
     9901 tgccatgctg gcccagcgct cgcagcagac caacgtcaag ctccgcgacc tcgccgctcg
     9961 attcgtcgag gaggccgcga agataccgct accgaatgcc gcgcgcctcg acgacatcct
    10021 ggcgaacgcg cacgagggcc tcggcgagcc ccgacaatga cgacatggcc tcctacttgc
    10081 tcaacgaagc cggcgtggcc aaggcacgca cgctgatcga tgcccgccag tacgttctcg
    10141 acagcgactg gggcgaggtg cagccgaaag ccgacgcgca gaattcctac ctcgattcgc
    10201 actcctggga cgaatacgcc gagtggcatc tcgggctgac agagggtgcg acggacgaga
    10261 ctaaggcgcg atatgccttc gtgtacgggg attttcgtcg aatccaccgc accggcctca
    10321 tcgcctgcgt gtaccgggcg tccgaatggc ggcacaagga cgtcgagctc gccgcccacg
    10381 acttgctgca atacctcgac aagaagaccg gtttggagcc gacgacccgg gcgtgaccgg
    10441 ctacggccgc agcatgaagt ccaggaagcg gtcctgcaga tcgggcgcag tggcgggggc
    10501 cgaccccgac gaatgcaggc tcccgatgcc caacgcgaag accacatagg accgcaaggc
    10561 cgcctcctcg cgcccgaacc cgacatcgac gaacgcctgc ctgaccgcgc gcagcacccg
    10621 agcgtcgctc tgctggacgc tgcccagcac ctcgtcgtcg gtcagcgccc atacccgcat
    10681 cgctcgctcg agctgccagt gccgcggtgc tacccacgtc tgaatcatca cggtcaggcg
    10741 ctcgcgcggg tcgacgtccg gcatcgtctc gaaatgttgc cggtcgcggt cgtggaggcc
    10801 gctccacgcc ttgatgagcg cgccgcggta cgcgggcata tccgtgaagt gccagtagaa
    10861 gctgcctttg gtgacgttga gccgctcgca gagcgagtcg acccgcagcg cgttgggccc
    10921 gccctcggcg agcacagcaa atccggcctc gatccagtcc tcggccgtga gccggcgggg
    10981 ggaaccgcgc cgcggcgcca tgctgggcag catacggcca ttaacgggta gcaccactga
    11041 caccagcgaa aattgcgtga gggaggggtg ctggcaaccg tggccgccag gtagggcgcc
    11101 gccgctcttc ggctcctact cccggcccgc ccggccggag gcagcgagat gtgcgaaaaa
    11161 tctcgagtgc cttcgcggcg gccacggttg ctacagcagc taaccgtacc atacggcctg
    11221 gtacggacag atgatctcag tttgcaaggg cctgccgcac aacacttctg cgcgttatgt
    11281 ggcagggggt ggtgtcaggc aacatgggcg gcatggcgcg tgacgacttc gacgacaacc
    11341 cgctggagcc cgccgaggaa ctcgactcgg acgaagtccg caatgccgac ggtgacgagg
    11401 tcgtcgaccc tccggacgag tggatcgagg ccaaggacaa cgaaaccctc gacgagcggc
    11461 tcgccgagga agtgcccgat gatcagggcg atgacgaggc cgacgacgcg cgcagcgacg
    11521 cccacgactc cgacgcgggc ggagggctgc ggctggtcag caactacgac atcgaccata
    11581 tggatccgca gacgcacggc cgtgagcgcg gacagatcga cggaacaccg gaggatgggg
    11641 actcgttcta cgacgtcatc gagtgaggtc ggtcctgtgg cgtttagtga cagccgcagt
    11701 gggcaaccga acagacgtgg atagcgacga caccctccgc cccgcggaag ccctggattc
    11761 cgacgacgtc cgcaacgaag acggcgacga ggtcgtcgac ccgccggagg gttgggcagc
    11821 ggcggaccat atcgccgagg agcccgaggg cgagtcactc gacgagaaac tggccgccga
    11881 ggaacccgac gcgtccttcc cggcacccga cgaggcagag gacgtcgacc acgtcgatcc
    11941 ggcccaccac ggcaccgacg agggccagat cagcggctcc ccggaggacg gcgactcgat
    12001 cttcccggtc gtcgagtgag ctaccccggg gtctggtcgc tggcctgcag caccagcacc
    12061 gcgcgcgcat cgaccgcgac cttggcgccc gcggtcaccg gcttggcttc ctcggcagtg
    12121 gcggtgtccg ccgtgtagat caccggcacc cacgctgagc cgaattcctt tggcggcagc
    12181 gagaattcga tcggttcgta gtgggcgttg aaacacagca cgaacgagtc atcggtgacg
    12241 cgctgtcctc gcacatcgcg accgggtatg ccctggccgt tgaggtacac ggcgatcgac
    12301 ttggcatagc ccgtttccca gtcgtccccg gtcatctgcg agccgtccgg tgcgaaccat
    12361 gcgatgtcgg gctgaccgcc gtccccgcgt cggccgaccg gcttaccgct gaagaaccgg
    12421 cgcctgcgaa acaccgggtg cgccgtgcgc aacgccgaca ccgaccgcgt gaattccatc
    12481 aactcggcgt cggcgttcgc ccaatcgatc caggacgttt cgttgtcctg acagtagacg
    12541 ttgttgttgc cctgctgcgt acggccgagc tcgtcgccgt gcgcgatcat cggaacgcct
    12601 tgcgaaagca gaagtgtggc gaggaaattg cgctgctgcc gcgcgcgtaa cgcgttgatc
    12661 tccggatcct tcgtcggccc ttccgcgccg cagttccagg accggttgtg gctttctccg
    12721 tcgcggttgc cctctttgtt ggcctcgttg tgcttttcgt tgtaggacac cagatcccgg
    12781 agcgtgaacc cgtcatgggc gatgacgaag ttgatcgacg cgacgggtcg gcggccggtc
    12841 tgctcgtaaa gatcggccga tccggtcagg cgataggcga actcgtcaag ggtggcaggc
    12901 tcaccgcgcc agtagtcacg cacggtgtcg cggtacttgc cgttccattc cgaccagagc
    12961 ggcgggaagt ttccgacctg gtagcccccc tcgccgacgt cccacggctc ggcgatgagc
    13021 ttcacctggc tcacgatcgg atcctgctgc acgaggtcga agaacgccga cagccggtcg
    13081 acctcgtgga actgccgcgc gagcgtcgcc gccaggtcga agcggaagcc gtcgacgtgc
    13141 atctcgcgca cccagtagcg gagcgagtcc atgatgagct ggagcgcgtg gggatgccgc
    13201 atgttgaggc tgttccccgt gcccgtcgtg tcgtagtagc ggccgcggtc ctcgggcacg
    13261 agacggtagt aggcggcgtt gtcgatgccc ttgaacgaca gcatcgggcc gaggtggttc
    13321 ccctccgcgg tgtggttgta gacgacgtcc aggatcacct cgatgccggc gtcgtggagc
    13381 gcccgcacca tgcccttgaa ctcctggacc tgctcgccgg tctgcccgga gctcgcgtac
    13441 tcgttgtgcg gcgcgaagaa gccgatcgag ttgtagcccc agtagttccg gagcccgcgc
    13501 tcgacgagct gcgcgtcgtg gacgaactgg tggatgggca gcagctccac ggccgtcacg
    13561 ccgagcgagc ggaagtagtc gatcgcgacc ggatgcgcga ggccggcgta ggtcccgcgg
    13621 agatgcctcg ggatgcccgg gtggcgcgcg gtgaagccct tcacgtgcac ctcgtagaag
    13681 acggactcgt gcagcgggat gtcgggcggg cggtcgttcc gccagtcgaa gaaaggaacc
    13741 gacacgacgc tcttcggcac gtgcggcgcg ctgtcctcgt cgttgcgttt gctgtcgtcg
    13801 ccgaactcgt aggggaagca cgccgggcgc cagtcgacct ggccctcgat cgcctttgca
    13861 tacgggtcca gcagcagctt cgcgggattg cagcggacgc cctcctcggg cgcccagcgg
    13921 ccgtggacgc ggtagccgta gcgctggccg aagccgacgg tcggcaggta ggcgtgccag
    13981 cagaacgcgt cgcgctcctc gagcgccacg cgctgctcgc tgccgtcggc gtcgaacagg
    14041 cagacatcga ccttctcggc cacctctgag aagagcgaga agttcgtgcc agcgccgtcg
    14101 tacgtcgccc caagggggta cgaacgtccg ggccacacct cgagctcggg cctcacggct
    14161 cctcctcgcg catcacggcg gccagctccc gcggcagggg ctccgcctcg agcgtctcga
    14221 gcggcgcacg cagcgcgagc gaccagttcg gccgccgtcc gtcggcgccg ggtacgttcg
    14281 ggcgctccac ttcgccgacg gcatcctcca gtgtgactgc gacgaggcgt gacggcgcgc
    14341 gcgccaccgt ccggtaggcc gcgaggatc
//
LOCUS       DASRJA010000059         7713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4177535,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000059 DASRJA010000000
VERSION     DASRJA010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7713
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4177535"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFC01_02605"
     CDS             complement(<1..331)
                     /locus_tag="VFC01_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HZN78567.1"
                     /translation="MTVNIIAVTFDEQSKAYQALSTLKGLDREGRVEVKSAAVVERDA
                     TGQLRIPEGADNASGEGFAIGGLIGMLVGILGGPVGMLLGLGTGGLIGGLDDLDKADK
                     EDQTLAQV"
     gene            complement(521..1117)
                     /locus_tag="VFC01_02610"
     CDS             complement(521..1117)
                     /locus_tag="VFC01_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HZN78568.1"
                     /translation="MTTFETPRVLQNVWKTVLAWAVLTLILGVVILVWPGISIEVAAV
                     LFGAYLIVAGLAQVMFAFALDVPGGERVLLFISGALSLVLGVLAFRHLGEGYAILLLA
                     IWIGVSFIFQGVAETTLAITYKELPGRGWHIFLGVLSVIAGIVVMAWPFDSIVLLAIV
                     AGGWLVVIGIFAVVWALQARKEIHTAERKIERLASADR"
     gene            complement(1123..1314)
                     /locus_tag="VFC01_02615"
     CDS             complement(1123..1314)
                     /locus_tag="VFC01_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78569.1"
                     /translation="MRSDIEDWQAERKEKDAERAAENAAEDAVVAVTLAAYCLDAAEW
                     AAVRAELARGEADELAGKS"
     gene            complement(1765..3195)
                     /locus_tag="VFC01_02620"
     CDS             complement(1765..3195)
                     /locus_tag="VFC01_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN78570.1"
                     /translation="MSTAIVVGAGPNGLAAAVTLAKAGVDVTVLEAADEIGGGTRTIE
                     AILPGLLHDHCSAIHPMAVGSPFLAGLGLERYGLTWRLPEIDCAHPLDGGRAGVLHRS
                     VEATADGLGSDHWRWRRLFSSTSRRFDALSDDIMGPLLRVPHHPLTLARFGAPTLLPA
                     STVARWFRTTEARALFGGVAAHAFRPLHFPMTSAIGLGILTAGHRHGWPVAAGGSRSI
                     TDAIASLLADLGGKVETGVRIDAASQLPPADVTMFDLAPEAVAGILGDRLPSRIASAY
                     RNFRRGPGAFKVDFAVEDGVPWSNAAARHAGTVHLAGTYAELAATERDVHAGRMPKRP
                     FVLVGQQYLADPLRSVGSIHPVWAYAHVPNGYTGDATAAIIAQIERFAPGFRDRVIGQ
                     TVSTTTQMAAHNLNFVGGDIMTGAKDFRQLVFGPRITLSPYRIGVPGMYICSAATPPG
                     PGAHGMCGAHAGQTALSDLSTHGQAS"
     gene            complement(3192..4985)
                     /locus_tag="VFC01_02625"
     CDS             complement(3192..4985)
                     /locus_tag="VFC01_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HZN78571.1"
                     /translation="MSFTKPEFPPVDPDTFLAKPLMERMRFLTQHWTDNGFGSPRMVH
                     AIYIAKLVFFYALGGVFVATVTSGLPAFWHVAEWWNQPIVYQKAVLWTVLLEAIGVAG
                     SWGPLAGKVKPMTGGILFWLRSDTIRLRPWKWVPFTGGDRRTWFDGILYFALLISVAV
                     PLLASGVHSDSLSQALPHNTSGLVNPALLVAPIVLLVLNGLRDKIIFLAARGEQYLPA
                     LFFFTVLPFVDMIIALKLLIVVVWVGAGVSKFGKHFANVIPPMVSNSPCMPFKWVKRA
                     HYRDFPRDLRPSRAAHFMAHVLGTTVEIAAPLVLLFSTNKGLTLAAVVVMVAFHLFII
                     STFPLAVPLEWNVLFAYAAVFLFLGFPSWHGYAVSNMSSPWLTATIVAALVFFPILGN
                     VRPDKVSFLPSMRQYAGNWASALWAFAPGAEAKLNSVTRTAKNQIDQFIAFGYEPKWA
                     EVTLQRAIAWRSMHSQGRGLFSVLLRTLPDIDARTVREAEFVCNSLIGFNFGDGHLHN
                     EDLIAAVQRQAGFEPGELVVVWAESQGWGSKVQHYKVIDAALGVIERGTWKVSDAVKE
                     QPWLPDGPIRTNVTWSVAGTREPRPSYPSMA"
     gene            complement(4988..6424)
                     /locus_tag="VFC01_02630"
     CDS             complement(4988..6424)
                     /locus_tag="VFC01_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN78572.1"
                     /translation="MDLSALPDRRATNDPLGQAIADDISDLNNVEFLDAVKRTAATLR
                     SRGVSAGDVVAIKLPNTVEFVVSLFAAWRVGAAVTPVNPALSPQEVRYQLADAGAKLV
                     IADELHQCEPAQADASRGSDASLALLIYTSGTTGRPKGVMLDHANLKAMCRSVIKGFR
                     LTRDDHSLLILPLFHVNGIVVGTLSPLLAGGRATVAGRFDPKTFFAALEQSRATYFSA
                     VPTIYTMLCGLPDDVQPDTSSVRFAICGAAPASVELLEGFESRYGIPIMEGYGLSEGT
                     CASAVNPLDGPRKPGTVGLPLPGQTIRIVDEAGRPVPDGDTGEVVIKGANVMRGYLNR
                     PEETAKTIVDGWLHTGDIGCFDEDGYLVLVDRAKDMIIRGGENIYPREIEAVVHHLPQ
                     IAEAAVVGRSNPTYGEEPVLFVSTRPNATVTPQAIRDHIGGQLAKFKRPADITILDDL
                     PRNPVGKIDKPALRRLLADSLATSTDGS"
     gene            6593..>7713
                     /locus_tag="VFC01_02635"
     CDS             6593..>7713
                     /locus_tag="VFC01_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HZN78573.1"
                     /translation="MTSGTDTDVDRCVRQIAAGLHQRLGELTAVIQRLLQDEIPELAL
                     DAQSIELLGASVGGNVDTVLHAMRYGIAVQRVEAPTAAMEYARRLAQHGVPVHGLVRG
                     YRVGQRRMNELVFAEVQATDMEPIVKLAVLEKMSATMFAYIDWMSQQVVEVYEEERER
                     WLENQNSLRALRVREILAAKTPVDVDAASTTIRYPLRWHHLAVVAWYPDAGSDGNELP
                     RLQRFLRDMGEAAGVAASPLFVAANAVCGWGWLPFRDAAPEAVDKVREFAATHADSPS
                     VGIGSMAAGIGGFRRSHRRALAARAVGAAREPHKPSVVAADDPGVLVAAMLGGDIIVA
                     REWVSEVLGDLAVDNENDARLRETLLVFLSGDGSYKLAA"
BASE COUNT         1445 a         2505 c         2450 g         1313 t
ORIGIN      
        1 cgacttgcgc cagcgtttgg tcttccttat cggccttgtc gagatcgtcc agaccgccga
       61 tgagcccgcc ggtacccaac ccgagcagca tcccgacggg accgccgagg atgccgacca
      121 gcattccgat cagcccgccg attgcaaagc cctcaccgct ggcattgtcg gcgccctctg
      181 gtatccgaag ttgcccggtt gcgtcgcgtt ctacgaccgc cgcgctcttg acttctacgc
      241 ggccctcgcg gtcaaggccc ttgagggtgc tcagcgcttg atacgccttg ctctgctcat
      301 cgaaagtcac agcgattatg ttcacggtca tatttttgct cctttgattc gaccgaatct
      361 cgccgaccgt ttatcggcga gacatctggg gttggggcga cggcgcccac gtcgacgagc
      421 cgggcggaaa tcccgaaccg tccctggcgg gagatcctct cgcatgtcga atctccttgg
      481 tttacacgta tattcaagac gcaaggtcga cggtcacggt ttagcggtcc gcacttgcca
      541 accgttcaat ctttcgctcg gcggtgtgga tttccttgcg ggcctgcagc gcccagacga
      601 ccgcgaagat tccgatgacg acaagccatc cgccggccac gatcgccagc aggacgatgg
      661 aatcgaacgg ccaggccata accacgatgc cggcgatgac gctcaggacg cccaggaaga
      721 tgtgccagcc acggccgggc agctccttgt aggtgatcgc cagcgtcgtc tccgcgaccc
      781 cctggaagat gaacgagaca ccaatccaga tggccagcag gagaatcgcg tacccctcgc
      841 cgagatgacg aaacgccaac acgcccaata ccaatgacag cgcaccgctg atgaacagca
      901 agacccgttc gccgccgggg acatcgagcg cgaacgcgaa catcacctgt gcgaggcccg
      961 ccacaatcag gtaggcgcca aacaataccg ccgcgacctc gatcgaaatc ccaggccaaa
     1021 cgagtatcac aacgcccaga atcagggtca gcacagccca cgccaacact gtcttccaca
     1081 cgttctgcag cactcgcgga gtttcgaagg ttgtcatggt gatcagctct ttccggctag
     1141 ctcgtcggcc tcgccgcgag cgagttccgc gcgtacggcg gcccactccg cggcatcgag
     1201 gcagtaggcg gcgagggtga cggcgaccac cgcatcctcc gccgcgttct cagccgcccg
     1261 ctcggcgtcc ttctccttgc gctcggcttg ccaatcctcg atgtcggagc gcatctccgc
     1321 gatctgccac tcgatagagc ctttggtatc tgaccaccag ctccgtgcgc cttccttggc
     1381 ttggcttgtg gcttgttcaa gctcaccctt ctcgcgctca atgacggccc gagctcgtca
     1441 cggcgtgcgt gcaacgcggc gcgattcttt tcctggaccg ccgccgccga gtcctcgagt
     1501 ctcttgactc gtccggcgag atccatcaac gactcagata gagtcttcat gttctgctcc
     1561 tccctagatt tgtgtatctg acaccaaaag tctgcgcctc gtaatgggcc cgacctaggg
     1621 cagaaagtca catcataagg tgattgatac tcgaactccc aagttagaca aggcttctac
     1681 tacaatcgtc aacgctgaat gatcgtcaca atcgacgaac ctccagccga tccgcggatg
     1741 gcagctgcgc gtgtggcgac cgagttacga agcttgcccg tgagtgctga ggtcgctgag
     1801 tgcggtttgt ccggcgtggg cgccgcacat gccgtgtgca ccggggccgg gcggcgtggc
     1861 agccgagcag atgtacatac ccggaacacc aatgcggtac ggcgacaacg tgattcgcgg
     1921 accaaacacc aactggcgaa aatccttggc gccggtcatg atgtcgcccc cgacgaagtt
     1981 gagattgtgt gcggccattt gtgtggtggt gctgacggtt tggccgatga cacggtcgcg
     2041 gaaccctggg gcgaagcgct cgatttgagc gatgatggcc gcggtggcat cgcccgtata
     2101 accgttgggc acgtgcgcgt aggcccaaac gggatggata ctgccgaccg aacgtagtgg
     2161 atcggccaaa tactgttgtc cgacaaggac gaacggtcgc ttcggcatgc gaccggcgtg
     2221 aacgtcccgc tcggtggcgg ccagttcggc ataggtgccc gccaggtgca ccgtgcctgc
     2281 gtgacgggcg gccgcgttcg accacggcac cccgtcctcg acggcaaagt cgaccttgaa
     2341 cgcgccgggc ccgcggcgaa aattccggta ggcgcttgca attcgcgaag gcagccgatc
     2401 gccgagaatg ccggcgacgg cctcaggtgc gaggtcgaac atcgtcacat cggcgggtgg
     2461 cagctgactc gccgcgtcga tccgcacccc cgtttcgacc ttgccgccca ggtcggccaa
     2521 cagcgatgcg atcgcgtcgg tgatcgatcg cgaacccccg gccgcaaccg gccacccgtg
     2581 acgatgcccc gcggtgagga tgcccagccc gatcgccgag gtcatcggaa agtgcagcgg
     2641 tcgaaacgca tgcgccgcaa cccctccgaa caatgcgcgc gcctcggtcg tacggaacca
     2701 cctggcaaca gtggatgcgg gcagcaaggt cggcgcgccg aaccgcgcca gtgtcaacgg
     2761 atggtgcggc accctcagca gcggccccat gatgtcgtcg gatagcgcgt cgaagcgtct
     2821 ggaggtcgag gagaacagcc gacgccaccg ccagtgatcg gacccgagcc cgtcggcggt
     2881 ggcctccacc gagcggtgga gcacgcccgc ccgcccgccg tcaagcggat gcgcgcagtc
     2941 gatctccggc aggcgccatg tcaacccgta acgctccagc ccgaggccgg ccagaaacgg
     3001 tgacccaacg gccatcggat ggatcgccga gcagtggtca tgcagcaagc ctgggaggat
     3061 cgcctcaata gtgcgggtgc ccccgccgat ctcgtcggcg gcttccagca cggtcacatc
     3121 cacgcctgcc ttggccaagg tgaccgcggc cgccaggccg ttgggcccgg cgccgacgac
     3181 gatcgccgtg ctcatgccat tgaagggtac gagggcctag gttcacgcgt gccggccacc
     3241 gaccacgtga cgttggtacg gatcgggcca tcgggcagcc acggctgctc cttgaccgcg
     3301 tcagacacct tccaggtgcc gcgttcgatc acacccaacg ccgcgtcgat gaccttgtag
     3361 tgctgcacct tgctacccca accttgcgac tccgcccaga ccacaaccag ttcacccggc
     3421 tcgaatcccg cctgccgctg taccgcggcg atgaggtcct cgttgtgcag gtgcccgtcg
     3481 ccgaagttga agccgatcaa cgagttgcag acgaattcgg cctcccgcac ggtgcgggcg
     3541 tcgatgtcgg gcagcgtcct caacagcacc gagaacaagc cacgaccctg actgtgcatc
     3601 gagcgccagg cgatggcgcg ctgcaacgtc acttctgccc acttcggttc gtagccaaag
     3661 gcgatgaact ggtcgatttg gttcttcgcg gtgcgggtga cgctgttcag tttcgcctct
     3721 gcccccgggg cgaacgccca cagggctgac gcccagttgc cggcatattg gcgcatcgac
     3781 ggtaggaacg acaccttgtc cgggcgcacg ttgcccagga tcgggaagaa gaccagcgcc
     3841 gcaacgatcg ttgccgtcag ccacggcgac gacatgttcg acacggcata cccgtgccag
     3901 gacgggaacc cgaggaacag gaaaaccgct gcgtaggcga acagcacgtt ccactccaac
     3961 ggcaccgcca gcgggaacgt cgagatgatg aacaagtgga aggccaccat gacgacgacc
     4021 gccgccaacg tcaacccctt gtttgtcgag aacaacagca ccagcggggc ggcgatctcc
     4081 acggtggtgc cgagcacgtg ggccatgaag tgggcggccc gcgatggccg cagatcgcgt
     4141 gggaagtcgc ggtaatgcgc ccgcttgacc cacttgaacg gcatacaggg gctgttgctg
     4201 accatcggcg ggatgacgtt ggcgaagtgt tttccgaact tcgacacacc ggcaccgacc
     4261 cacaccacga cgatcaacag cttcagcgcg atgatcatgt ccacgaacgg cagcacggtg
     4321 aagaagaaca gcgccggcag gtactgctca cccctcgcag cgaggaagat gatcttgtcg
     4381 cgaaggccgt tcaacaccag cagcacgatc ggcgcgacca gcagcgcggg gttgacgagc
     4441 ccggatgtgt tgtggggcaa agcttgtgac aatgagtcgc tgtgtactcc ggaggccagc
     4501 agcgggacgg cgacgctgat cagcagggcg aagtagagga tcccgtcgaa ccacgtgcgg
     4561 cggtcaccgc cggtgaacgg cacccacttc cacggccgca accggatggt gtctgaacgg
     4621 agccaaaaca ggatgccgcc cgtcataggc ttgaccttgc cggccaacgg accccatgaa
     4681 ccggcgacgc cgatcgcctc gagcagcacc gtccacagga ccgctttctg gtaaacgatc
     4741 ggttggttcc accactccgc gacgtgccag aacgccggca gacccgacgt caccgtcgcg
     4801 acgaacacac caccgagggc gtagaagaac accagcttcg cgatgtagat cgcatgcacc
     4861 atgcgcggag acccgaagcc gttgtcggtc cagtgctggg taaggaagcg catccgttcc
     4921 atcagcggct tggcaaggaa ggtgtctgga tcgaccgggg gaaactccgg cttcgtgaaa
     4981 ctcatgatca gctcccgtct gtggatgtgg ccaatgagtc ggccagcagc cgacgcaatg
     5041 cgggtttgtc gatcttgccg acggggttcc tgggcaggtc gtcaaggatc gtgatgtctg
     5101 ctggccgttt gaatttcgcg agctgcccgc cgatgtgatc gcgaatcgcc tgtggggtga
     5161 cggtcgcgtt cggacgtgtg gagacgaaca ggaccggctc ctcgccgtag gtggggttcg
     5221 atcgcccgac gacggcggcc tcggcgatct gcggcagatg gtggacgacg gcctcgatct
     5281 cgcgcgggta gatgttctcg ccaccgcgaa tgatcatgtc cttggcgcgg tcgaccagaa
     5341 ccaggtagcc atcctcgtcg aagcacccga tgtcgccggt gtgcaaccag ccgtcgacga
     5401 tggtctttgc ggtctcctcc ggccggttca gatagccacg catcacgttg gcgcccttga
     5461 tgacaacctc gcctgtgtca ccgtccggga ccgggcggcc cgcctcatcg acgatccgga
     5521 tggtctgccc gggcagcggc agtcctaccg tccccggttt cctcggaccg tccagcgggt
     5581 tgaccgcact ggcgcatgtt ccctccgaga gcccgtagcc ctccatgatg gggatgccgt
     5641 agcgcgattc aaatccttcg agcagctcga cgctggccgg cgcggctccg cagatcgcga
     5701 accgcaccga tgacgtgtcc ggttgcacgt cgtcgggcag cccgcacaac atcgtgtaaa
     5761 tcgttggtac ggcggagaaa taggtggcac ggctctgctc gagtgctgca aagaatgtct
     5821 tcgggtcgaa gcgcccggcc accgtcgccc ggccaccggc cagcaacggc gacagcgtcc
     5881 cgacgacgat cccgttgacg tgaaacagcg ggagtatcaa caggctgtgg tcatcgcggg
     5941 tcaacctgaa acccttaatg acgctgcggc acatggcctt caggttcgcg tggtcgagca
     6001 tgacgccctt ggggcggccg gtggttccgc tggtgtagat cagcaacgcc agcgaggcgt
     6061 cgctgccccg actggcgtcg gcttgagctg gctcgcactg gtgcaactcg tcggcgatca
     6121 ccagcttggc accggcgtcg gcaagctgat agcggacctc ttgcggcgac aatgcggggt
     6181 tgaccggcgt gaccgccgca cccacccgcc acgcggcaaa cagcgacacc acgaactcga
     6241 ccgtgttcgg cagcttgatc gccaccacgt cacccgccga cacgccccgg ctgcgcagcg
     6301 tcgcggcggt gcgcttgacg gcgtcgagaa actcgacgtt gttgaggtcg ctgatgtcgt
     6361 cggcgatcgc ctgccccagc ggatcattcg tcgcacgccg atcaggcaag gccgacaagt
     6421 ccatgtaatt tcctcccaga tgggctaccc gtgtgaccgg caacactacg aagcgcgggg
     6481 acgaatcggc atgatctggg ccgctcaaat accgggcacc gtttgtcggc cggagacaac
     6541 cccgagcgct catttggccc acaagcgagt taggaagatc taccgtcgtg gtgtgacgag
     6601 cggcacagac accgatgtcg accgctgtgt ccgccagatc gctgccggac tccaccagcg
     6661 gctgggcgaa ttgacggccg tcattcagcg tctactgcag gacgagatcc ccgagcttgc
     6721 gctggacgcg cagagcatcg agctgttggg ggccagcgtc ggaggcaacg tcgacactgt
     6781 gctgcacgcg atgcggtatg gaattgcggt gcagcgtgtc gaggcgccga ccgcggcgat
     6841 ggagtacgcc cggcggttgg cccaacacgg agtcccggtg cacgggttgg tgcgtggcta
     6901 ccgggtcggt cagcggcgca tgaatgaact cgtcttcgcc gaggtccagg ccaccgacat
     6961 ggagccaata gtaaagctgg ccgtgctcga gaagatgtcg gcgaccatgt tcgcgtacat
     7021 cgattggatg tcacagcaag tcgtcgaagt gtacgaggag gagcgcgaac gctggctgga
     7081 gaatcagaac agccttcgcg cgttgcgggt gcgcgagatt ctggccgcca agacacctgt
     7141 ggacgtcgac gccgcgtcga cgacgattcg ctatccgctg cgctggcatc atctggcggt
     7201 ggtggcctgg taccccgatg ccggcagcga cggcaacgaa ctgccacggc tgcaacgctt
     7261 cctgcgagat atgggcgaag ccgctggggt ggcggcgagc ccgctgtttg tggccgccaa
     7321 cgccgtatgc ggctggggat ggctaccctt ccgcgacgcg gcacccgaag cagtcgacaa
     7381 agttcgcgag ttcgcggcca cccatgcgga ctcgcccagt gtgggaatcg gctcgatggc
     7441 ggcgggaatc ggcggatttc gacgatcgca tcgcagggcg ctggccgcgc gggcggtggg
     7501 ggcggcccgc gagccgcaca agccgagtgt cgttgccgcg gacgatcccg gcgtgttggt
     7561 ggccgccatg ctgggcgggg acatcatcgt ggcacgtgag tgggtgtccg aagtcctcgg
     7621 cgacctggcc gtcgacaacg agaacgatgc ccggctgcgc gagacgttgc tcgtcttcct
     7681 gtccggcgac ggcagctaca agctcgctgc tga
//
LOCUS       DASRJA010000060        12332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2484470,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000060 DASRJA010000000
VERSION     DASRJA010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12332
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484470"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="VFC01_02640"
     CDS             <1..128
                     /locus_tag="VFC01_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HZN78574.1"
                     /translation="ADLVKANKAADGISLDIARQTSPVPLHPGAKKALDELGAAS"
     gene            125..2203
                     /locus_tag="VFC01_02645"
     CDS             125..2203
                     /locus_tag="VFC01_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HZN78575.1"
                     /translation="MTESEHDKDRPGPVGEVAAPTAYEDEKPARQLTGWVRHAVAAAC
                     VLVGLFAVYQVFFPLAQGNQVSLILFLAAVLPLTLLCYRSGLRLRRRRNAPPERDNPT
                     PVDWLLAAVALAACLYPVLPFEFADGGGGFDEFLSRQGMPAMVDVIVGSIVVLLVLEA
                     CRRTTGLALPIVCLLALGYAYYGGYLPQGAVIAHAGVDWDQIISASFIQSTGLYGVPL
                     DVAATYIVLFTIYGAVLDASGASKFFIDLSFAAFRRSRAAPGRTVTLAGFLLGSVSGS
                     GTATTVSLGSVCWPVLRRAGYPSENAGGMLAAAGIGAILSPPTLGAAAFIIAEYLGVP
                     YLTVLVWAIVPTILYYLGILLAVEIDARRYATREVEVTAQSTWRLLGRFGYHLISLVM
                     IVVFLAMDMSAVRAVVYATAVQIVLSFLDREHRLTPARLYSALATGVRGVLSVAAVCA
                     AAGVIAAMITRTGLGLQLNSILVNTARTITENPTAVLILTAVFAALAVSVLGLAVPVT
                     ASFVISWVIVGPALQDLGVPAPAVAMFVFYFAVLSEVTPPTALAAVAASAITGGRVIA
                     TMWQTWKYTLPAFLVPFAFVLTDNGEGLLGLRSLGDMAWTAAVAGLAVAALAVVTGGW
                     IIRPAGWPERLLCVPAAALLLYLNPATIAVGVGLLAVAVGVHLVISRRPQAESPDPSH
                     PGSRGTADRS"
     gene            complement(2127..2573)
                     /locus_tag="VFC01_02650"
     CDS             complement(2127..2573)
                     /locus_tag="VFC01_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN78576.1"
                     /translation="MTEGRLRNSKLLAVLLKYFARAHIWVYRRTNGRLGARLLWFPAA
                     LVTTKGRKTGEPRTTPTLYLTDGDRVVLPASFGGRDSHPAWYRNLKANPQVHIQIRGE
                     HFDLVARDASDEERERYWARLIRIYPPYRGYRDATDRVIPLVVCER"
     gene            complement(2576..3304)
                     /locus_tag="VFC01_02655"
     CDS             complement(2576..3304)
                     /locus_tag="VFC01_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78577.1"
                     /translation="MTDNYDLHYYFDPVCPFAWMTSKWVRMVASQRDYRLDWRFISLR
                     ILNAHIDYAAHFPPEYEEGHTAGLRLLRVAARVRAEQGRDAVGPLYEALGTRIFDTSR
                     EVDPLSASDQGARRTLEPLLGDVGLPPDLADALDDESYDEVIRAETEEALALTGRDVG
                     TPILHFQPPDGTAFFGPVISRLPSAEDAGPLWDHVIALAAFPGFAELKRSLRERLQLV
                     SFGVDPDSVGKEEDWHGGSRRLKK"
     gene            complement(3301..3756)
                     /locus_tag="VFC01_02660"
     CDS             complement(3301..3756)
                     /locus_tag="VFC01_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN78578.1"
                     /translation="MPKSRPRFMDTKAADFFIKWMSKINTYMYRRSDGEGFGSNFQGI
                     PVALLTTTGRASGEPRISPLYFHRDGDIVIVAASKGGSDKNPMWYLNLKANPKVQVQI
                     KKEILDLTARDATDEERAKYWPRLVEMYPTYDDYQSWTDRQIPLVVCEP"
     gene            complement(3766..4140)
                     /locus_tag="VFC01_02665"
     CDS             complement(3766..4140)
                     /locus_tag="VFC01_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78579.1"
                     /translation="MPATDAERIAAAQAYIDALVSHDADKVPFAPGCTRIEIGLKTGF
                     SGNHLRRSLNRGPQYRVIRETTVPEFTVDGDRVRARFDLFTKPSVAGRRVGAHIDETF
                     LIPAEDGAIHHIKAVVRPFLTS"
     gene            complement(4140..5303)
                     /locus_tag="VFC01_02670"
     CDS             complement(4140..5303)
                     /locus_tag="VFC01_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HZN78580.1"
                     /translation="MGNPVIVEATRSPIGKRNGWLSGLHATELLGATQKALVEKAGID
                     AGDVEQIVGGCVTQFGEQSNNITRVSWLVAGLPEHVGAMTVDCQCGSGQQANGLVAGL
                     IAAGAIDIGIACGIEAMSRVGLGANAGPDRGILRPASWDIDLPDQFTAAERIAKRRGI
                     TREEIDQFGFDSQRKAKQAWAEGRFDREISPIEAPVLDENKQPTSERAPVTRDQGLRD
                     TTLEGLASLKPVLEDGIHTAGTSSQISDGAAAVLWMDEDKARALGLKPRARIISQALV
                     GAEPYYHLDGPVQSTAKVLEKAGMKIGDIDLVEINEAFASVVLSWARVHEPEMERVNV
                     NGGAIALGHPVGSTGSRLITTALHELERTDQSTALITMCAGGALSTGTIIERI"
     gene            5425..6672
                     /locus_tag="VFC01_02675"
     CDS             5425..6672
                     /locus_tag="VFC01_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN78581.1"
                     /translation="MPCPIPADFDFLDATLNLERLPVAELAELRKSEPIHWVDVPGGT
                     GGFGDKGYWLVTKHSDVKEVSKRNDVFGSSPDGAIPTWPQGMTRDAIDLQKAVLLNMD
                     APQHTRLRKIISRGFTPRAVGRLEDELRDRAQKIAQTAAAEGTGDFVEQVSCELPLQA
                     IAGLLGVPQEDRDKLFRWSNEMTAGEDPEYAHIDPAMSSIELIQYAMQMADERSKNPT
                     DDIVTQLIEADIEGEKLSDDEFGFFVVMLAVAGNETTRNSITHGMIAFSQHPEQWELY
                     KKERPETAADEIVRWATPVSAFQRTALEDVELSGVQIKKGQRVVMSYRSANFDEDVFE
                     NPHAFDIMRSPNPHVGFGGTGAHYCIGANLAKMTINLIFNAVADAMPDIKPIGEPERL
                     MSGWLNGIKHWQVDYAGKCPVAH"
     gene            6698..7696
                     /locus_tag="VFC01_02680"
     CDS             6698..7696
                     /locus_tag="VFC01_02680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN78582.1"
                     /translation="MDFSPDEGQQAVADVVTSVLDRDNSWDALVAGGVTALAVPERLG
                     GDGVGLSEIATALTEIGRHGTVSPALASIGFGTAVLLDLASDEQQDRYFAGVAKGSVL
                     TAALNEPGAPLPDRPTTTFAGGKLNGTKIAVPYAQQAEWIIVTADSAVVVVSSKADGV
                     QMTKTPASNGGDEYTVTFSDAEAAGVLEGAEAHRVNQMALAAIGAFASGLVAGALRLT
                     ADYVANRHQFGKPLSTFQTVAAQLAEVYIASRTLTLAATSVVWRLAEGRDADDDLDVL
                     GYWLASQAPPVMQICHHLHGGMGMDIDYPMDRYYSTVKDLTRLVGGPSHRLDLVGA"
     gene            7696..8859
                     /gene="fadE29"
                     /locus_tag="VFC01_02685"
     CDS             7696..8859
                     /gene="fadE29"
                     /locus_tag="VFC01_02685"
                     /EC_number="1.3.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE29"
                     /protein_id="HZN78583.1"
                     /translation="MFIELTPEQRQLQAELRQYFSNLISPDEYKEMEADRHGKAYRAV
                     IKRMGSDGKLGVGWPKEFGGLGYGPIEQSIFVNEAHRADVPLPAVTLQTVGPTLQVFG
                     SEAQKKKFLPAILAGELHFAIGYTEPEAGTDLASLRTTAVRQGDEYIVNGQKVFTTGG
                     HDADYIWLACRTDPEAVKHKGISILIVDTKDPGYSWTPMILSDGAHHTNATYYSDVRV
                     PADMLVGEENGGWRLITTQLNNERVMLGPAGRFAALYDRIHAWASKPGGNGDIPLDHD
                     DVKRSLGELKAMWRINELLNWQVAASGETIDVADAAATKVFGTERIQYAGRLAEEIVG
                     KYGNPAEPDTAELLRWLDSQTKRNLVITFGGGVNEVMREMIAASGLKVPRVPR"
     gene            8856..9812
                     /locus_tag="VFC01_02690"
     CDS             8856..9812
                     /locus_tag="VFC01_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional MaoC family dehydratase
                     N-terminal/OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HZN78584.1"
                     /translation="MSVEDIQKAAEKVKAEGKSKPRVGRHPVNQPMVDHWLDAMGDKN
                     PIYVHEEAAKAAGHPGIVAPPAMIQVWTMMGLGGIRPDDDPLGKILGLFDDAGYIGVV
                     ATNCEQTYHRYLRPGEEVSVAAELTDVVGPKKTALGEGFFITQKITWQVGDEDVAEMM
                     WRIMKFKPAESDGGAEVPEDLDADTMMRPASSKDTKFFWDGVNAHELRIQKRPDGSLQ
                     HPPVPAIWQDKEASIEYQVASGKGTVFSFVVHHAPKVPGRTLPFVIALVELEEGVRML
                     GELRNVDPAKVEIGMPVRAIYIDFPEGDSGPAWTLYAWEPDE"
     gene            9809..10210
                     /locus_tag="VFC01_02695"
     CDS             9809..10210
                     /locus_tag="VFC01_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZN78585.1"
                     /translation="MSAPTVEVGTKLPELALYGDPTFIVSTAIATRDYQDVHHDRDKA
                     QAKGSKDIFVNILTDTGLVQRYITDWAGPTAIIKSISLRLGVPWYAYDTVTFRGEITA
                     IDGDLITLKVVGSNSLGDHVIATATLTIGAA"
     gene            10210..11376
                     /locus_tag="VFC01_02700"
     CDS             10210..11376
                     /locus_tag="VFC01_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HZN78586.1"
                     /translation="MPGELSGKAAIVGIGATDFSKNSGRSELRLAAEAVLDALDDAGL
                     TPADVDGMVTFTMDSNTEVAIARATGMGELKFFSKIHHGGGAACATIQQAAIAVATGV
                     AECVVAYRAFNERSGVRFGQVQMRLVENADSTGVDNSFSYPHGLSTPAAQVAMIAQRY
                     MHLSGATSKDFGAVSVADRKHAAKNPKAYFYEKPITIEDHQNSRWIAEPLRLLDCCQE
                     TDGAVAIVVTSAERAKDLKHRPAIIEAASQGSSPDQYSMVSYYRPELGLPEMGLVGRQ
                     LWQQSGLKPTDIQTAILYDHFTPFTLIQLEELGFCGQGEAKDFIADGAIEIGGRLPIN
                     THGGQLGEAYIHGMNGIAEGVRQLRGTSVNPVPDVEHVLVTAGTGVPTSGLILG"
     gene            11497..12273
                     /locus_tag="VFC01_02705"
     CDS             11497..12273
                     /locus_tag="VFC01_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78587.1"
                     /translation="MSSPTPPDPWQSQTSTPDDVHEPDPDDADTGPLPAQTPPLPAEE
                     RWHAGQGAWAEKFDAPLIVNPRPVRHHERKPIVFLGVVSLLIVVVVGALIFWLVRPSS
                     DTTSAPPPSAAPSTPSPDTDRLLRLVPAGYPADACKPTGAPKDALAQINCDKNSDLGG
                     PPTATYTLVANKAALDMAFNGIVRGSTRVNCPGNIQSPGPWRRNATPDKISGLLFCGV
                     QDGRPMVAWTDDARLVVSQVQSGQEGPTFDELYAWWSSHS"
BASE COUNT         2142 a         3934 c         4089 g         2167 t
ORIGIN      
        1 aggccgacct ggtcaaggcg aacaaggccg ctgacggcat cagcctcgac atcgcccgtc
       61 agaccagccc ggttccgctg catcccggcg ccaagaaggc gctcgacgaa ctcggcgcgg
      121 ccagctgacc gagagcgaac acgacaagga ccggccgggg ccggtcgggg aagtcgctgc
      181 gccgacggcg tatgaggatg agaaaccggc ccggcagctg acggggtggg tacgccacgc
      241 ggtggccgca gcctgtgtgc tggtcgggct gttcgcggtg tatcaggtgt tcttcccgct
      301 cgcccagggc aaccaggttt cgctgatctt gttcctcgcg gcggtgctgc cgttgacgct
      361 gttgtgttac cgctctggat tgcggctccg ccgccgccgc aacgcaccac cagaacggga
      421 caacccgacg ccggtcgact ggttgcttgc ggcggtggcg ttggccgcgt gcctctatcc
      481 cgtcctgcca ttcgaattcg cagacggcgg agggggattc gacgaattcc tgagtcggca
      541 gggcatgccg gcaatggtcg acgtgatcgt cggatcgatc gtcgtgctgc tggtgctgga
      601 ggcctgtcgc aggaccaccg gcctcgcgct gccgatcgtg tgcctgctcg cgctcggcta
      661 tgcctactac gggggctatc ttccgcaggg cgcggtgatc gcacacgccg gggtggactg
      721 ggatcagatc atcagcgcct cgttcatcca gtcgaccggt ctctatgggg tgccactgga
      781 tgtcgcggcc acctacatcg tgctgttcac gatctacggt gcggtgcttg acgcgtccgg
      841 cgccagcaag ttcttcatcg acttgtcgtt cgcggcgttc cgccggtcac gcgccgcgcc
      901 gggccggacc gtgacgctgg caggctttct cctcggcagt gtttcgggct ccgggaccgc
      961 gaccacggtg agtctcgggt cggtgtgctg gccggtgttg cgccgtgccg gttatccgag
     1021 tgagaacgcc ggcgggatgc tcgcggccgc aggtatcggc gcgatcctgt ccccgccgac
     1081 gttgggagcg gcggccttca tcatcgccga ataccttggc gtgccatacc ttacggtgct
     1141 tgtttgggcg atcgtgccga ccatcctcta ctacctcggc atcctgctcg cggtcgagat
     1201 cgacgcgcgc cgctacgcca cacgtgaagt cgaggtgacc gcgcagtcga cgtggcggct
     1261 gctgggacgg ttcggctatc acttgatctc cctggtgatg atcgtcgtgt tcctcgccat
     1321 ggatatgtcg gctgtccggg cggtggtgta cgcgaccgcg gtgcagatcg tgctgtcatt
     1381 tctcgaccgc gaacacaggc tcacccccgc ccggctgtat agcgcgctgg ccaccggcgt
     1441 ccgcggtgtc ctctccgtgg cggcggtctg cgccgcggca ggcgtcatcg ccgcgatgat
     1501 caccaggacc gggctgggcc tgcagctcaa ctccatcctg gtgaacaccg cgcgcacgat
     1561 caccgaaaac cccacggcgg tactgattct cactgcggta ttcgccgcgc tggcggtctc
     1621 ggtgctcggc ctggcggtac cggtgacggc gtcgttcgtg atcagctggg tgatcgttgg
     1681 accggccctg caggatctcg gggtacccgc cccggcggtc gcgatgttcg tcttctattt
     1741 cgcggtgctc tccgaggtta cgccgccgac ggcgcttgcc gcggtcgccg cgtctgccat
     1801 caccggcggc cgggtgatcg ccaccatgtg gcagacgtgg aaatacacgc tgccggcgtt
     1861 cctggtgccg ttcgcatttg tcttgacgga caacggcgaa ggactgctcg ggctgcggtc
     1921 gttgggcgac atggcgtgga cggcagcggt ggccgggttg gcggtggccg cgctcgccgt
     1981 cgtcaccggc ggctggatca ttcgacctgc tggatggccc gagcgactgc tgtgtgtgcc
     2041 agcggccgcg ttgctgctgt acctgaaccc cgcgacgatc gccgtggggg tcggcctgtt
     2101 agcggtggcc gtgggcgtgc acctcgtcat ctctcgcaga ccacaagcgg aatcacccga
     2161 tccgtcgcat cccggtagcc gcggtacggc ggatagatcc tgatgagcct ggcccagtag
     2221 cgctcgcgct cctcatcgct ggcgtcacgg gccacaaggt cgaaatgctc gccgcgaatc
     2281 tgtatgtgca cctgggggtt tgccttgagg ttgcgatacc acgccggatg gctgtcgcgt
     2341 ccgccgaatg atgcgggcag cacgacgcga tcaccgtcag tcagatagag ggtaggcgtg
     2401 gtccgtggtt cgccggtctt tcgtcccttc gtcgtgacca gcgccgccgg aaaccacagc
     2461 agcctggcgc cgagccggcc gttggtgcgc cggtaaaccc agatgtgcgc acgagcgaag
     2521 tacttcagca gcacggcaag tagtttcgag ttgcgaagcc ggccttccgt catgactact
     2581 tcttcaggcg gcgactgccg ccgtgccaat cctcttcttt gccaaccgaa tccgggtcga
     2641 cgccgaagct caccagctgc agccgctccc gcaggctccg cttgagctcg gcgaaaccag
     2701 ggaaggccgc cagcgcgatg acgtggtccc aaagcggacc cgcatcttcg gccgacggca
     2761 gcctgctgat cactggcccg aagaacgccg tcccgtccgg cggctggaag tgcaggatcg
     2821 gtgtgccgac atcgcgcccc gtcaacgcca gcgcctcctc ggtttcggcg cggatcacct
     2881 cgtcgtacga ctcgtcatca agcgcatccg cgagatcggg tggcaggcca acgtcgccca
     2941 gcaacggctc cagcgtgcgt cgcgcgcctt gatccgatgc ggaaagcgga tcgacctcac
     3001 gtgacgtgtc gaagatgcgg gtgccgagcg cctcgtagag cggcccgacc gcgtcgcgtc
     3061 cttgctccgc acgcacccgc gcggcgactc gcagcagccg tagcccggca gtgtgcccct
     3121 cctcgtactc tggagggaag tgcgccgcat agtcgatgtg tgcgttgaga atccgcagcg
     3181 agatgaaccg ccagtccagt cggtagtcgc gctgcgatgc caccatccgc acccacttgc
     3241 tcgtcatcca ggcaaacggg cagacgggat cgaagtagta gtgcaggtcg taattgtctg
     3301 tcacggttcg cagaccacga gggggatctg ccgatcggtc caggactggt agtcgtcgta
     3361 cgtcgggtac atctcgacca gccgcggcca gtacttggcg cgttcttcgt ccgtcgcgtc
     3421 acgggcggtg aggtcgagga tctctttctt gatctgaacc tgaaccttcg gatttgcctt
     3481 gaggttcaga taccacatcg ggttcttgtc actgccgccc ttggacgccg caactatcac
     3541 gatgtcgccg tcgcggtgga aatagagcgg actgatgcgc ggctctccgc tcgcgcgccc
     3601 ggtcgtcgtc agtagcgcga cgggaatgcc ctgaaagttg ctgccgaagc cctcgccgtc
     3661 gctgcgccgg tacatgtacg tgtttatctt cgacatccac ttgatgaaga agtccgcggc
     3721 cttcgtatcc atgaagcggg ggcgagactt tggcatggct cgatcctagg acgtcaggaa
     3781 gggacgaacg accgccttga tgtggtggat tgcgccgtcc tcggcgggga tgaggaacgt
     3841 ctcgtcgata tgagcgccga cccgccgacc cgcgacggac ggcttggtga acaggtcgaa
     3901 ccgagcccgg acccgatcgc cgtcgacggt gaattcgggc accgtggtct cccggatgac
     3961 ccggtactgc ggtccgcgat tcaggctgcg gcgcaggtga ttccccgaga atccggtctt
     4021 gaggccgatc tcgatgcggg tgcagccggg cgcgaacgga accttgtcgg cgtcgtgact
     4081 gaccagcgca tcgatgtagg cctgcgccgc cgcaatccgt tccgcgtccg tagccggcat
     4141 tagatccgct cgatgatcgt gccggtcgat agcgcaccgc ccgcgcacat tgtgatcagc
     4201 gctgtgctct gatcggtgcg ctccaactcg tgcagtgcgg tggtgatcag ccgactgccg
     4261 gtgctgccga cggggtgacc gagcgcgatc gcgccgccgt tgacgttgac ccgctccatc
     4321 tccggctcat gcacgcgcgc ccacgacagc acgaccgacg cgaaggcctc gttgatctcg
     4381 acgaggtcga tgtcgccgat cttcatgccg gccttttcca acaccttcgc cgtcgactgg
     4441 accgggccgt cgaggtggta gtacggctcc gccccaacca gcgcctggct gatgatccgg
     4501 gcccgcggct tcaacccgag cgcgcgcgcc ttgtcttcgt ccatccacag caccgcggcg
     4561 gcgccgtcgg agatctgcga cgaggtgccc gcggtgtgga taccgtcctc gagaacaggc
     4621 ttcagcgacg ccagcccttc gagcgtggta tcgcgcaggc cctggtcgcg ggtgacgggt
     4681 gcgcgctcgg acgtcggctg cttgttctca tcgaggacgg gcgcctcgat cgggctgatc
     4741 tcgcggtcga accggccctc ggcccaggcc tgcttggcct tgcgctgcga gtcgaagccg
     4801 aattggtcga tctcctcgcg ggtgatgccg cggcgcttcg cgatgcgctc ggcggcggtg
     4861 aactggtcgg gcaggtcgat gtcccacgat gcgggccgga gtatgccgcg atcagggcct
     4921 gcgttggcgc cgaggccgac gcggctcatc gcctcgatgc cgcacgcgat gccgatgtcg
     4981 atggcaccgg ccgcgatgag gccggcgacc aggccgttgg cctgctgacc gctgccgcac
     5041 tggcagtcga cggtcatcgc gccgacgtgc tcaggcagcc cggcgaccag ccagctcacc
     5101 cgggtgatgt tgttggactg ctcgccgaac tgcgtgacgc agccgccgac gatctgctcg
     5161 acgtcgccgg cgtcgatgcc ggccttctcg acgagtgcct tctgcgtggc gcccagcaac
     5221 tcggtggcgt gcagaccgga gagccagcca ttgcgcttgc cgatggggct gcgggtggct
     5281 tcgacgatga cagggttacc cattccgtca ggctagaaca cgtttcatta ctctgacaag
     5341 cgaggatggt gacctgcctt ttatctgcgc agaaggcatg ttttactggc actagaacac
     5401 gttgcaattg aggagagctt tcctatgccc tgccccatcc cagctgactt cgacttcctc
     5461 gacgcgaccc tgaatctcga gcgtctgccc gttgcagaac tggccgaatt gcgcaagtcc
     5521 gagcctatcc actgggtcga cgtgcccggc ggtaccggcg gcttcggcga caagggctac
     5581 tggctcgtga ccaagcactc cgacgtcaaa gaggtgtcca agcgcaacga tgtcttcgga
     5641 agctctccgg acggcgccat tccgacatgg ccgcagggta tgacccgcga cgcgatcgat
     5701 ctgcagaagg ctgtcctgtt gaacatggac gcgccgcagc acaccaggct gcgcaagatc
     5761 atctcccgcg gtttcacgcc gcgcgctgtc gggcgactgg aggacgaact gcgggaccgc
     5821 gcccagaaga tcgcccagac cgccgcggcc gagggcaccg gtgacttcgt cgagcaggtc
     5881 tcctgtgagc tgccgctgca ggccatcgcc gggctgcttg gcgtcccgca ggaagaccgc
     5941 gacaagctct tccgctggtc caacgagatg accgccggtg aggacccgga gtacgcccac
     6001 atcgatccgg cgatgtcgtc gatcgagctg atccagtacg cgatgcagat ggccgatgag
     6061 cggtcgaaga atcccaccga cgacatcgtc acgcagctga tcgaggccga tattgaagga
     6121 gagaagctct ccgacgacga gttcggcttc ttcgtcgtca tgctcgcggt cgccggcaac
     6181 gagaccaccc gcaactcgat cactcacggg atgatcgcgt tctcacagca tcccgagcag
     6241 tgggagcttt acaagaagga acgtccggag actgctgccg acgagatcgt tcgttgggcc
     6301 acgccggttt cggcgtttca gcgcactgcc ctcgaggatg tcgagctgag cggggtgcag
     6361 atcaagaagg gccagcgggt ggtgatgtca taccgctcgg ccaacttcga cgaagatgtg
     6421 ttcgagaacc cgcatgcctt cgacatcatg cggagcccga acccgcacgt gggattcggc
     6481 ggcaccggcg cccattactg catcggcgcc aaccttgcca agatgaccat caacctgatc
     6541 ttcaacgcgg tcgccgacgc gatgcccgac atcaagccga tcggcgagcc ggagcggctc
     6601 atgtcgggct ggctcaacgg catcaagcac tggcaggttg attacgccgg taagtgccca
     6661 gtcgcgcact gacgcactga gactcaagga ggagtcggtg gacttcagtc cagacgaggg
     6721 gcagcaggct gtcgccgatg tggtgacatc cgtgctggac cgggacaaca gctgggacgc
     6781 gctggtcgcc ggaggtgtga cggcgctcgc cgtgcccgag cgcctcgggg gtgacggcgt
     6841 cgggctgtcc gaaatcgcga cggcgctgac cgagatcggc agacacggca ccgttagccc
     6901 ggcgctggcc agcattgggt tcggcactgc ggtgctgctg gatctggcgt ctgacgaaca
     6961 gcaggaccgc tacttcgccg gcgtcgcaaa gggttcggtg ctgaccgcgg cattgaacga
     7021 gcccggcgca ccgctgccgg accggccgac gaccaccttc gccggcggca agctcaacgg
     7081 tacgaagatc gcggttccct acgcgcaaca agcggagtgg atcatcgtga ccgcggacag
     7141 cgccgtcgtg gtggtgtcat cgaaggctga cggtgtgcag atgaccaaga cgccggcgtc
     7201 gaatggcggc gacgagtaca ccgtcacctt ctccgacgca gaggccgccg gggtgctgga
     7261 gggcgccgaa gcccaccggg taaaccagat ggcgctcgcg gcaattggtg cgtttgcatc
     7321 cgggctcgtt gcaggtgcgc tgcggttgac cgccgactac gtggccaatc gccatcagtt
     7381 cggcaagccg ctgtcgacgt tccaaacggt ggcggcacag ctagcggagg tctacatcgc
     7441 ctcgcgcaca ctgacattgg cggcgacgtc ggtcgtgtgg cggctcgccg agggccgtga
     7501 cgccgacgat gatctcgacg tcctcggtta ctggttggct tcacaggcac cgccggtgat
     7561 gcagatctgc caccacttgc acggcggcat gggcatggac atcgactatc cgatggatcg
     7621 ctactactcg accgtcaagg acctgacccg gttggtgggc gggccgtcgc atcgtctcga
     7681 tctggtggga gcgtaatgtt catcgaactg acccccgagc agcgacagct tcaagccgaa
     7741 ctgcggcaat acttttcgaa cctcatctcc cctgacgaat acaaggagat ggaggccgat
     7801 cggcacggca aggcctaccg cgcggtcata aagcggatgg gttctgacgg caagctcgga
     7861 gtgggctggc ccaaggagtt cggcggcctg ggctatggcc cgatcgaaca gtcgatcttc
     7921 gtcaacgagg ctcaccgcgc agacgtaccc ctgcccgccg ttaccttgca aacggtggga
     7981 ccgacactgc aagtgttcgg gagcgaagcg cagaagaaga agttcctacc cgccattctg
     8041 gccggcgagt tgcactttgc gatcgggtat accgagccgg aggcgggcac cgaccttgcg
     8101 tcgttgcgca ccaccgcggt tcggcagggc gacgagtaca tcgtcaacgg gcagaaggtc
     8161 ttcaccaccg gaggacatga cgccgactac atttggctcg cgtgccgcac agatccggaa
     8221 gcagtaaagc acaagggcat ctcgattctc atcgtcgata cgaaggatcc cggctactcc
     8281 tggacgccga tgatcctgtc cgacggcgca caccacacaa acgccacgta ctacagcgac
     8341 gtccgggtac ccgccgacat gcttgtcggt gaggagaacg gcggctggcg cctgattacc
     8401 actcagctca acaacgagcg cgtgatgctg ggccccgcag gtcgtttcgc cgcgctttac
     8461 gaccggatcc acgcgtgggc gtcgaagccg ggtggcaacg gtgacatccc gctcgaccac
     8521 gacgacgtca agcgctcact tggtgaactc aaggccatgt ggcggatcaa cgagctgctc
     8581 aactggcaag tcgcggcgtc gggcgaaacc attgacgtcg ccgacgcggc cgcgaccaaa
     8641 gtgtttggca ccgaacggat ccagtacgcg ggtcggctgg cagaagagat cgtcggcaag
     8701 tacggtaatc cggctgaacc ggacaccgcc gagctgttgc gctggctcga cagccagacc
     8761 aagcggaatc tggtgatcac gttcggcggc ggagtgaacg aggtgatgcg cgagatgatt
     8821 gcggcgtcgg gtctgaaagt gccgagggtc cccaggtgag cgtcgaagac atccagaagg
     8881 cggccgagaa ggtcaaggcc gagggcaaaa gcaagccacg ggtcggtcgc catccggtga
     8941 accagccgat ggtcgaccac tggctcgacg cgatgggcga caagaacccc atctacgtcc
     9001 acgaggaagc cgcgaaggcc gcgggccatc cagggatcgt tgcgccgccg gcgatgatcc
     9061 aggtgtggac gatgatgggt ctcggcggta tcaggccgga cgacgacccg ttgggcaaga
     9121 ttctcggatt attcgacgac gcaggctata tcggtgtcgt cgcgaccaac tgcgagcaga
     9181 cctaccaccg gtatctgcgc cccggtgagg aggtcagcgt cgcagccgag ctgaccgacg
     9241 tcgtgggccc gaagaagacc gcgctgggcg agggcttctt catcacgcag aagatcacct
     9301 ggcaggttgg cgatgaggat gtcgccgaaa tgatgtggcg catcatgaag ttcaagcctg
     9361 ccgagagcga tggcggggcc gaggtgcccg aggacctgga cgcagacacg atgatgcggc
     9421 ccgcgtcgtc caaggacacg aagttcttct gggacggcgt caacgctcac gagctgcgaa
     9481 tccagaagcg gcccgatggc agcctgcagc atcctccggt gcctgcgatc tggcaggaca
     9541 aagaagcatc gatcgaatat caggtggcca gcggcaaagg cacggtgttc agcttcgtcg
     9601 tgcaccacgc gccgaaggtg cccggtcgca cgctgccgtt cgtcatcgcg ctcgtcgaac
     9661 tcgaagaggg agtgcggatg ctcggtgagc tccgcaacgt ggacccggcc aaggtggaga
     9721 tcggaatgcc cgttcgcgca atatatatcg actttcccga gggcgattcg ggcccggcgt
     9781 ggacgctgta cgcgtgggag cctgacgaat gagcgcgccg accgtagaag tgggcacgaa
     9841 gcttcccgaa ctggcgctgt atggcgaccc gacgtttatc gtgtcgacgg cgatcgccac
     9901 gcgcgactat caggacgtcc atcacgaccg cgacaaggcg caggccaagg ggtcgaagga
     9961 catcttcgtc aacatcctga ccgacaccgg tctggtccag cgttacatca ccgactgggc
    10021 gggaccgacc gcgatcatca agtcgatctc gctgcggctc ggtgtgccgt ggtacgccta
    10081 tgacaccgtg acgttcaggg gcgagatcac cgccatcgac ggtgacctga tcacgctgaa
    10141 agtcgttggc agcaacagtc ttggcgatca cgtgatcgcg accgcgacac ttacgattgg
    10201 agctgcctga tgccgggtga gctgtcggga aaggcggcga tcgtcggcat cggtgccacc
    10261 gacttctcga agaattccgg ccgcagcgag cttcgactgg cggctgaggc ggtgctggat
    10321 gcgctcgacg atgcgggcct gacgccagcc gacgtcgacg gcatggtgac gttcacgatg
    10381 gactcgaaca ccgaggtcgc catagcgcgg gccaccggca tgggcgagct gaagttcttc
    10441 tccaagatcc atcacggcgg cggcgccgcc tgcgccacca ttcagcaggc ggccatcgcg
    10501 gtggcgactg gtgtcgcgga atgcgttgtc gcataccgtg ccttcaacga acgctcggga
    10561 gtgcggttcg ggcaggtgca aatgcgcttg gtggaaaacg ccgattccac gggcgtggac
    10621 aactcgtttt cgtatccgca cggcctgtcg acgccggcgg cgcaggtggc gatgatcgcc
    10681 caacggtaca tgcacttgtc cggggcgacg agcaaggact tcggcgcagt ttcggttgcc
    10741 gaccgcaagc acgcggccaa gaaccccaag gcctacttct acgagaagcc gatcaccatt
    10801 gaggaccacc agaattcgcg gtggatcgcc gaaccgttgc ggctgctgga ctgctgtcag
    10861 gagaccgacg gcgccgtcgc gatcgtcgtg acgtcggcgg agcgggccaa ggacctgaag
    10921 catcgaccgg cgattatcga ggccgcgtcg cagggctcga gcccggatca gtattcgatg
    10981 gtcagctact accgcccgga gctgggtctg cccgaaatgg gcctggtcgg caggcaactg
    11041 tggcagcagt cgggcctgaa gccgaccgac atccagaccg cgattctgta tgaccacttc
    11101 acccccttca cgctgatcca gctggaggag ttgggcttct gcggtcaggg cgaggcgaag
    11161 gacttcatcg ccgacggcgc catcgagatc ggtggcaggc tgccgatcaa cacccacggc
    11221 ggccagcttg gcgaggcgta catccacggc atgaacggca tcgccgaggg tgttcgccag
    11281 ctgcgcggta cgtcggtgaa tccggtgccc gacgtggagc atgtgctcgt gaccgcgggc
    11341 accggtgtgc cgacgtccgg cctgattctg ggctagccca ctcctcctac taacctcata
    11401 ccggcagccc ggccatcgct aggctgccgt aagtggttgt cgccgcgatg atcatggggg
    11461 ttgtgtagtg aacatgagcc acacggaggt acgggcatga gttcgcccac accgccggac
    11521 ccgtggcagt ctcaaacttc gacgcctgac gacgtgcacg aaccggaccc cgacgacgcc
    11581 gacacggggc ctctgcccgc ccagacgcca ccgctgccgg ccgaggagcg atggcacgcc
    11641 ggccaggggg catgggcgga gaagttcgac gcaccgttga tcgtaaaccc gcgaccggtg
    11701 cggcatcacg aacgcaaacc gattgtcttc ctcggcgtcg tctcgctgct gatcgtggtc
    11761 gtcgtcggcg ccctgatctt ctggctggtg cggccgtcgt ccgacacgac cagtgcaccg
    11821 ccgccgtcag ccgcaccgag tacgccgagt cccgacaccg atcgcctctt gcgcctcgtg
    11881 ccggccggtt atccagccga cgcgtgcaag ccgaccggtg cgccgaaaga cgcactcgcg
    11941 cagatcaatt gcgacaagaa ctccgacctg ggcggcccac cgaccgcgac ctacacattg
    12001 gttgcgaaca aagccgcgct cgacatggcg ttcaacggca ttgtccgggg gtcaacgagg
    12061 gtcaactgcc ccggcaacat tcagtcaccg gggccgtggc gccgcaacgc gacgccagac
    12121 aagatcagtg gcttgttgtt ctgcggcgtg caggacggcc gcccgatggt ggcatggacc
    12181 gacgacgcca ggctcgtcgt cagtcaggtc caatcaggcc aggaagggcc aactttcgac
    12241 gagctgtacg cgtggtggtc aagccactcg tagcggaccc ctcagcctgc gggtgcagga
    12301 gcaggcggaa ccgttgtcgt cgtcaccggt gt
//
LOCUS       DASRJA010000061        11204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2484215,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000061 DASRJA010000000
VERSION     DASRJA010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11204
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484215"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFC01_02710"
     CDS             <1..213
                     /locus_tag="VFC01_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN78588.1"
                     /translation="AAMLSFALSLDDFIITYFVSGSTVTYPLYVNAAVKAAVPPQINV
                     LATAILVISLLLLAVGTLYRRKRIET"
     gene            complement(210..560)
                     /locus_tag="VFC01_02715"
     CDS             complement(210..560)
                     /locus_tag="VFC01_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HZN78589.1"
                     /translation="MARGIFDTPVVGIVNKGFVGLINAPVIGRFVGRGLVVIRYVGRR
                     SGQTFEIPVGYRRSGDTLTINVGAPDSKNWWRNFLGEGGPITLLNLDGADRTGHAVAS
                     RDEKGRVSVSVTLN"
     gene            complement(578..1444)
                     /locus_tag="VFC01_02720"
     CDS             complement(578..1444)
                     /locus_tag="VFC01_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZN78590.1"
                     /translation="MMTNQARTGALMAMGSMLCVQVGLAIAVTLIDRIGVEGAAWLRL
                     AWAGVLMLVIVRPRFASFTWATFRVCVVLGVVTAAVTLLFMAAVDRIPLGTASALEFL
                     GPLGVAVAHGKGSHRVVWPGLAAIGVVLLTEPWTGAVDPIGVLYALGAAACWACYILL
                     TQRAGDEVSGINALAVSMPVAGLVATIAVGPWVLPRITPDILLIGIGLAILLPVVPFA
                     LELLALRRLTTAAFGTLMALEPGFAMIVGLVVLHQIPGPAGIVGICFVVAAGIGAART
                     GARSSPVPAEVG"
     gene            complement(1532..2509)
                     /locus_tag="VFC01_02725"
     CDS             complement(1532..2509)
                     /locus_tag="VFC01_02725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN78591.1"
                     /translation="MKHITLGKLDVSRIGLGAMSMSGYYNTGEGSEAESIRTIHRALD
                     LGVTHIDTAEIYGPYLNEELVGGAIKGRRDQVVVATKFGLVSHSGGGPGVVDSSPANV
                     RAAVEGSLKRLGVDHIDLYYQHRVDPNTPIEDTVGVLAELVAEGKVRHIGLSEAGVET
                     IRRAHAVHPITALQTEYSLWTRDVEAELLPLLRELGVGFVPYSPLGHGFLTGQIRSTA
                     DISDDDWRKTNPRFVGENFQRNLRIVDEVEAVAAEVGVTPAQIALAWLLAQGDDIAPI
                     PGTKRVARVEENTAADGVELSSDQVERLNNLTPASGERHDEGNMAAIDR"
     gene            complement(2592..3623)
                     /locus_tag="VFC01_02730"
     CDS             complement(2592..3623)
                     /locus_tag="VFC01_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017572520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN78592.1"
                     /translation="MAACANKPEEISNRSDDAMRTRNLGALEVSEIGAGCMSISANYG
                     PPAGRNQGINTIRTAYENGVTFFDTAEVYGPYTSEELVGEALSPFRDKVTVASKFGFD
                     VEGAGGPLSRPEHIKKVVEGSLQRLRTDRIDLYYQHRVDPTVPIEDVAGAVKDLISEG
                     KVLHFGLSEAGARTIRRAHAVHPVAAVQSEYSVWTRDPERNGVLDTCEELGVGFVPWG
                     PVGLGYLTGKIDARARFDPQTDLRSRFPRFAPEALAANMPVVDLMKRFAEKKNATPAQ
                     IALAWLLAQKPFIVPIPGTRNIDHLTENLGALNVAPTPADLQEIDSDFSKITVHGARM
                     DEANMQIVE"
     gene            3843..4730
                     /locus_tag="VFC01_02735"
     CDS             3843..4730
                     /locus_tag="VFC01_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN78593.1"
                     /translation="MDSRNEIREFLATRRARITPQQAGLATYGRRRVPGLRREEVAML
                     AGVSTDYYARLERGNLTGVSDSVLEALARALRLDEAERAHLRDLARVANTTSHTRRRP
                     APKQHIRPVVQRILDGMTAMPAIVLTGRLDLLAANRLGVALYSPVYADPTRPVNLARF
                     CFLNPYATALYPHWDDAADTTVAMLRIEAGRNPYDRDLSDLIGELSTRSETFRNRWAA
                     HNVVLHRAGAKQFHHPVVGDLSLAYEVMELSADTGLTLTAYSPEPNSPSEDGLALLAS
                     WAATLDQTDQEQTTYPTDR"
     gene            4830..5807
                     /locus_tag="VFC01_02740"
     CDS             4830..5807
                     /locus_tag="VFC01_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009476367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HZN78594.1"
                     /translation="MAAWYDRQGPAGEVLQVGDLPDPQPGPGEVRVRVHLSGVNPGDT
                     KKRRGWLGSSMPFPRVIPHSDAAGVIDIAGDAVDKSRVGQRVWVYGAQSYRPFGTAAQ
                     FAVVPEQLAVPLPDGLGDDVGAALGIPGITAHRAVFSDGPVDGKTLLVHGVLGGVSSL
                     AAQLARWGGATVIATVTRSSDLARVDPAVVAHAVALDKSDPAGVIREFAPKGVDRIVE
                     VAFSENADLDAAVVGNDAVIAAYATRADRPEFPFWPMLFANVTIRLLGSDDFPAGAKQ
                     QAARDLTSAAAADALHIEVGQSYPLHQIADAHDHVDAGTRGRVLVHIPQ"
     gene            6012..6956
                     /locus_tag="VFC01_02745"
     CDS             6012..6956
                     /locus_tag="VFC01_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN78595.1"
                     /translation="MDTRRLALLLALSRLGSMRKVAEAFALTTSTVSQQIAALGREAG
                     EQLVEPDGRRVRLTPAGRRLADHAVTILAAVDAARLDLNPDAEPIGTVRVGGFATGIR
                     LSLLPIVRELAATHPRVEVAINEYEPLEAFELLVDDDLDLAITYDYNLAPASPDTVLE
                     SVPLWSTPWGLGVPADAATNAVTDLSAFADSTWIINSRNTADEDAVRTLAALAGFTPR
                     IVHQIDSLDLVEDLILDRHGVGLLPVNRPTRDGVAILRLSDPSVILTAYAVTRRGRST
                     WPPLRAILDRLRPPNQSLPQEDWPRPTAHPRVADGNES"
     gene            7423..7971
                     /locus_tag="VFC01_02750"
     CDS             7423..7971
                     /locus_tag="VFC01_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinucleotide-binding protein"
                     /protein_id="HZN78596.1"
                     /translation="MKIVVIGRGHVGGGLARLWSSRGHQVTALGRDGGDATDAEVVVI
                     AVAAVQIPDALDAVTGLAGQPTIDACNIYTDRQHEFPSLSHQIQSIIGGPTAKAFSTN
                     FASAYGRIAEQRVAPSNLYAADDDARESAEQLSIDAGYQPVYVGPLDPGARLLEDSSG
                     LTRALAAQLGPFFYRYAQPGQL"
     gene            7971..8921
                     /locus_tag="VFC01_02755"
     CDS             7971..8921
                     /locus_tag="VFC01_02755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZN78597.1"
                     /translation="MRAVKFTRYGDPDVLEVVEVPYPAAGPGQVVVAVVTAATNPGEI
                     GIREGRFADIWPAHFPEGQGNDFAGYVAEMGSGVDDFAVGDEVLGFAPRAAQAEYVAL
                     NVDRVTAKPAGLSWEAAATIAGAGATAWASVTAVNPQPGETIVVSAAAGGVGIYAAQL
                     ARLRGADVIGTCGPANVDLLTSLGIRPAQYGEGLFQLLRDLAPDGIDGYIDTFGSGNV
                     SLAISLGVQPHRINTLIDQEAVQRFGVHSEAQEQADTPGVWAELADLVARKSITVPIA
                     AVYDFSTEQVRQAYHDVGARHVSGKRVLRIMPADVQACHT"
     gene            complement(8912..9349)
                     /locus_tag="VFC01_02760"
     CDS             complement(8912..9349)
                     /locus_tag="VFC01_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN78598.1"
                     /translation="MSAADYAVMVPLSEAPNGQLRTKDLGIALGWDRSRTSHQVTRMV
                     KRGLVFREFCEEDARGSVVGLTPAGRTAIEKAAPNHVALVRQLFFDPLSDHELDALGE
                     ALNRMLAVIRQYTAEQPSDEGDHGDRLPNRSRAKRRNGIERHV"
     gene            9525..9950
                     /locus_tag="VFC01_02765"
     CDS             9525..9950
                     /locus_tag="VFC01_02765"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZN78599.1"
                     /translation="MTHPDAEALKAVVRRNTEQVQGQGDFALFDELFADDFVDHTPQP
                     GTTADKAGVRVLYKRLRDAFPDFRPEIHWQTVDGDIVTTYKIYHGTHLGDFLGIAATG
                     KAIHFETVDAMRVRDGTITDHWGVANLYSVLQQLGQLPT"
     gene            complement(9939..10823)
                     /locus_tag="VFC01_02770"
     CDS             complement(9939..10823)
                     /locus_tag="VFC01_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN78600.1"
                     /translation="MSDRRPSELAEFLSVRRARLTPQDAGLEGQAARRRVPGLRREEL
                     ARLAGVSVDYYTRLEQGRSRSASTDVLDALAAALQLNDAERQHLHLLAKPQPAQRKRR
                     SRPQSVDPATLRLLELLDQAWSPAFVLGRRLDVLAHNRLAGALITEFRELPAPLRNQA
                     RFVFFDPHARELYADWNEVAADTVAMLRLDAGRYPDDEKLSALVGELSIQSVEFRSWW
                     SNHNVERRTTGTKAYHHPLVGDLTVKYQALNPSGDPDQVLIVYTTEPGSADETALRLL
                     ANWHQGDRHSEPYTRSGR"
     gene            10979..>11204
                     /locus_tag="VFC01_02775"
     CDS             10979..>11204
                     /locus_tag="VFC01_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN78601.1"
                     /translation="MSTVITGAPLAGRVAIVTGASSGIGEATAFRLATLGAKVVVAAR
                     RKDNLDALVARITAAGGTALALPLDVTDRSA"
BASE COUNT         1963 a         3707 c         3509 g         2025 t
ORIGIN      
        1 gcggccatgt tgtccttcgc gctgtcgctc gacgacttca tcatcacgta tttcgtcagt
       61 ggttcgacgg tgacctatcc gctgtacgtg aatgcggcgg tgaaggccgc cgtgccaccg
      121 cagatcaatg tgctggcgac cgcgatcctg gtgatcagcc tgctcctgct ggcagtggga
      181 acgctgtacc gccgcaagcg aatcgagacc tagttcagcg tcacgctgac cgacacgcgg
      241 cccttctcgt cgcgactggc gaccgcatgc cccgtgcggt cggcgccgtc caggttgagc
      301 agtgtgatgg gtccgccctc accgaggaag ttgcgccacc agttcttgga gtcgggcgcc
      361 ccgacattga tcgtcagcgt gtcgcccgat cggcggtacc ccactgggat ttcgaaggtc
      421 tgcccggagc ggcggccgac atagcgaatc acgaccaacc cgcggccgac gaagcgtcct
      481 atgacggggg cgttgatgag tcctacaaag cctttgttga cgatgcccac cacgggggta
      541 tcgaagatgc cgcgtgccat gtcccgagac tacgtggtca gccgacctcg gcgggcaccg
      601 gcgacgagcg cgcaccggtg cgggctgcac cgatgcctgc ggctaccacg aagcagatgc
      661 cgacgatgcc cgccgggccg gggatctggt gcagcaccac taagccgacg atcatcgcga
      721 accccggttc gagtgccatc agggtgccga atgcggcggt ggtcaaacga cgtagcgcta
      781 gcagttcgag tgcgaacggc accaccggca acaggatcgc caggccgatt ccgatgagca
      841 ggatgtccgg cgtgatccgc ggcagcaccc atgggccgac ggcgatggtc gcgacaagac
      901 cggcgacggg catcgagact gccagcgcgt tgatgccgga cacctcgtcg ccggcgcgct
      961 gggtaagcag gatgtagcag gcccagcagg cggccgcgcc gagcgcgtac aaaacgccga
     1021 tcgggtcgac ggcaccggtc cacggctcgg tcagcaggac gacgccgatc gccgcgagtc
     1081 ccggccatac gacgcggtgg ctgcccttgc cgtgtgccac cgcgacgccg agcgggccga
     1141 ggaattccag tgcgctggcc gtgcccagcg gaatgcgatc cactgccgcc atgaacagga
     1201 gcgtgacggc cgcggtcacc acacccagca cgacgcagac gcggaaggtg gcccaggtga
     1261 acgacgcgaa gcggggccgg acgatgacga gcatcagcac gccggcccac gccagccgta
     1321 gccacgccgc accctcgacg ccgatgcggt cgatcagcgt cacggcgatc gccaggccga
     1381 cctgtacgca cagcatcgag cccattgcca tcagagcgcc ggtgcgggcc tggttcgtca
     1441 tcactcctgc attgaacggc atcacaaccg ttcacgtcca cgtgattttc ctggacatac
     1501 cgttcatgaa tcatgaacgg tcgttccggg gtcagcggtc gatggcggcc atgtttcctt
     1561 cgtcgtggcg ttcgccggag gccggcgtga ggttgttgag ccgttcgact tggtcggagc
     1621 tcagttcgac gccatcggca gcggtgttct cctcgactcg ggcaacccgc ttcgtgccgg
     1681 ggatcggggc gatgtcgtcg ccctgcgcga gcagccaggc cagcgcgatc tgggcaggag
     1741 tgacacccac ctcggcggca acggcctcaa cctcatcgac gatgcgcagg ttgcgctgaa
     1801 aattctcccc cacaaagcgc gggttggtct ttcgccagtc atcgtcggaa atgtcggcgg
     1861 tggaacggat ttggccggtg aggaatccgt ggcccaacgg cgaatacgga acgaagccca
     1921 cacccagttc gcgcagcagc ggcagcagct cggcctccac gtcgcgggtc cacaatgagt
     1981 attcggtctg cagtgcggtg atcgggtgca cggcgtgggc gcggcggatt gtctcgaccc
     2041 cggcttccga cagtccgatg tggcggacct tgccctcggc caccagctcg gccaaaacac
     2101 cgacggtgtc ctcgatcgga gtgttcgggt cgacccgatg ttggtagtac aggtcgatgt
     2161 ggtcgacgcc taggcgcttc agggaaccct cgaccgcggc gcggacgttc gccgggctgc
     2221 tgtcgacgac gcccggcccg cccccggagt gagacacaag gccgaacttc gtcgccacca
     2281 caacctggtc gcgacggccc ttgatcgcgc cgccgaccag ctcctcgttg aggtaagggc
     2341 catagatctc ggcggtgtcg atgtgggtga cgccaaggtc cagtgcccgg tggatggtcc
     2401 ggatcgattc ggcctcgctg ccttcccccg tgttgtagta gccggacatc gacatggcgc
     2461 caagaccaat ccgggagacg tccagctttc ctagggtgat gtgtttcatc agttgctcca
     2521 attctcaatt tctcttgttc ccaggcgatt tcgggcctga agtcgggtgg aggtcgtcca
     2581 ctatcggacg gctattcgac aatctgcatg ttggcttcat ccatacgcgc accgtgcacc
     2641 gtgatcttgg agaagtcgct gtcgatctcc tgaagatccg cgggcgtcgg cgcaacgttg
     2701 agcgctccga ggttttcggt gaggtgatcg atgttgcgtg tgccggggat gggaacgatg
     2761 aacggtttct gcgccagcag ccatgccagt gcgatctggg caggtgttgc gttcttcttc
     2821 tccgcgaatc gtttcatcag gtcaacgacc ggcatgttcg ctgccagggc ttccggggca
     2881 aagcgcggga accgggatcg aaggtcggtt tgcgggtcga accgagcgcg cgcatcgatc
     2941 ttcccggtca ggtagcccaa tcccaccgga ccccacggga cgaagccgac gcccaattcc
     3001 tcgcaagtat cgaggacgcc gttgcgttcg ggatcgcgtg tccacactga gtattcgctc
     3061 tgcactgcgg ccacggggtg aacggcgtga gctcgtcgga tggttcgtgc acccgcctcg
     3121 gaaagaccga agtgcagcac cttcccctcg ctgatgaggt ccttgaccgc gccggccacg
     3181 tcttcgatcg gcacggtcgg gtcgacccgg tgttggtaat agaggtcgat gcggtcggtt
     3241 cgcagacgct gcagcgaccc ctcgaccacc ttcttgatgt gctcgggtcg gctcagcggt
     3301 ccacccgcgc cctcaacatc aaaaccgaac ttgcttgcaa cagttacctt gtcgcggaat
     3361 ggtgagagtg cttctccgac aagctcttcg ctcgtgtagg gaccataaac ttcggcggta
     3421 tcgaagaacg taacaccgtt ttcatacgcc gtgcggatgg tgttgatgcc ttggtttctg
     3481 cctgcgggag gtccgtagtt ggcgctgata ctcatacagc cggcaccaat ttcggacact
     3541 tccagtgctc cgagatttct ggttcgcatc gcgtcgtcgc tccggttgct gatttcctcg
     3601 ggtttgttcg cgcatgcggc caacaacggg ggcccgacca ccaaccccgc tccgacgagg
     3661 gcggttctcg ccatgaggtt gcggcggctg atagcccgga taccgtttgt ctcagccaat
     3721 tcggttgcct ccttcgtggc ctgagctcag gaaacctcac gcagggggcg ggtggcagtg
     3781 cctggttatc catgtaatgg cagaccaccc ctacagtcga tcggccgccg taacctggcc
     3841 gtgtggacag caggaacgag atacgcgagt ttctggccac ccgacgggcc aggatcacac
     3901 cccagcaggc ggggctggcc acctacggca ggcgacgcgt cccgggcctg cgtcgcgaag
     3961 aagtggcgat gctggcgggc gtcagcaccg actactatgc ccgtctggag cggggaaacc
     4021 tcaccggagt ctccgacagc gttctcgaag cccttgcccg cgccctgcgc ctcgacgagg
     4081 ccgaacgcgc acatctgcgc gacctagccc gcgtcgccaa caccacatcc cacacccgcc
     4141 gacgccccgc tccgaagcaa catattcgtc ccgttgtgca gcgcatcctc gacggaatga
     4201 ccgcgatgcc ggcaatcgtg ctcaccgggc gtcttgacct tctggctgcc aaccggctcg
     4261 gtgtggccct gtactcaccg gtgtatgccg acccgacccg gccggtgaac ctcgcgaggt
     4321 tctgctttct caacccatac gccaccgcgc tctatccgca ctgggacgat gccgcggaca
     4381 ccactgtcgc catgcttcgc atcgaagcgg gccgcaaccc atacgatcgc gacttgtccg
     4441 acctcatcgg ggagctatcc acccgcagcg agaccttccg caatcggtgg gccgcgcaca
     4501 atgttgtcct gcaccgagcc ggcgcgaaac agttccatca cccggtcgtg ggcgacctca
     4561 gcctcgccta cgaggttatg gaactctcgg ccgacactgg attgaccctc accgcctaca
     4621 gcccagaacc caacagccca tcggaggacg gtctggctct tctcgcaagc tgggccgcca
     4681 cccttgatca gaccgatcag gaacaaacaa cctatccgac agatcgatga gatccctagc
     4741 ggcgcatcgc cgctctgtgc tgcgtaggcg gtttgatttt ctccaagcgc cggccagctt
     4801 cttctatcac ggtgaggaca ctcaatatga tggctgcgtg gtatgaccgg cagggcccgg
     4861 ccggtgaggt actgcaggtc ggcgatctac ctgacccgca gcccggtccg ggcgaggtcc
     4921 gcgtgcgtgt ccacctttcg ggggtcaacc cgggcgacac caagaagcgg cgcggatggc
     4981 tcggctcatc catgccattc ccgcgcgtga tcccccacag tgatgcggca ggtgtcatcg
     5041 acatcgctgg cgacgcggtc gacaagagcc gtgtgggaca acgggtgtgg gtctacggtg
     5101 cgcagtccta ccggcccttc ggcactgccg ctcagttcgc cgtggtcccc gaacagctcg
     5161 cggtgccgct gccagacggc ctcggcgacg atgtcggagc ggctcttggc attcccggta
     5221 tcaccgcgca ccgcgccgtc ttcagtgacg gaccagtcga cgggaagacg ctgctcgtgc
     5281 acggcgtgct cggcggcgtc agctccctag ccgcccaact cgcccgctgg ggcggagcaa
     5341 cggtgattgc caccgtcact cgcagtagcg acctcgctcg cgtcgatccc gcggtggtgg
     5401 cgcacgccgt cgccctcgac aaatccgatc cggctggcgt gatccgggag ttcgcgccga
     5461 aaggcgtgga ccgtatcgtc gaggtggctt tctctgagaa cgccgacctc gacgccgcgg
     5521 tggtcggcaa cgacgctgtc atcgccgcct acgccacccg cgccgacagg ccagaattcc
     5581 ctttctggcc aatgcttttc gccaatgtga ccattcgact gttaggcagc gacgatttcc
     5641 ccgccggtgc caagcaacag gccgcgcgcg acctcaccag cgcagcagct gccgacgcat
     5701 tgcatatcga ggtcggtcag tcttacccgc tacaccagat cgccgacgcc cacgaccacg
     5761 tcgatgccgg cacccgcggc cgcgtcctcg ttcacatccc gcaatagcca acacccacgc
     5821 cgttaacggc cggcgcagaa attgttcgaa acagtgtcgg ttttcaccga gaccgtttag
     5881 ccaaagtgga caataagcgg catggatatt cgccgccgag ggcgccacgt gtcgggtctc
     5941 agagtttgtg gcgaaatctc acgcgaaatg gcgaatggtg gcgaattcgc cacggataga
     6001 gcggacgtct agtggacacc cggcggttgg cgctgcttct ggccttgtct cgactggggt
     6061 cgatgcggaa ggtggccgag gccttcgcgt tgacgacgtc gactgtgtcg cagcagatcg
     6121 cggcgctggg ccgagaggcc ggtgagcagt tggtcgagcc ggatggccgg cgggtgcgcc
     6181 tcacgccggc agggcgccga ctggccgatc acgcggtgac cattctggcc gccgttgacg
     6241 ctgcgcgact cgacctcaat cctgacgcag aaccgatcgg cacggtgcgc gtcggtggct
     6301 tcgcgaccgg catccgcctc tcgttgctgc cgatcgtccg cgagttggcg gccacccatc
     6361 cacgggtcga ggtggcgatc aacgagtacg agccactcga ggcattcgag ctgctggtcg
     6421 atgacgacct cgatctggct atcacctacg actacaacct ggcacccgcc tcccccgaca
     6481 ccgtactcga atcggttccg ctgtggtcaa ctccgtgggg tttgggcgtt ccggccgacg
     6541 ctgcgacgaa cgctgtcacc gatctcagcg cgttcgcgga ttcgacttgg attatcaact
     6601 cccgcaatac agctgacgag gacgcggtac gtacgctggc ggcattggct ggtttcacgc
     6661 cgcgcatcgt ccatcaaatc gacagcctgg accttgtcga agatctgata ctcgatcgcc
     6721 acggggtagg cctactgcca gtcaatcgcc caacccgtga tggggttgcg atcctgagac
     6781 tctcggaccc cagtgtcatc ctcactgcgt acgccgtaac tcgacggggc cgatcgacgt
     6841 ggcctcctct tcgagcgatt ctcgatcgac tacgcccgcc gaatcagtca ctgccccagg
     6901 aggattggcc ccgtccgacg gcgcaccctc gcgttgcgga tggcaacgag tcgtagaccg
     6961 actgggttgc gcggccgtcg tcgatagcat gcaattgtgt tcactcatac gcgattcaac
     7021 tgcactgagc gttttcgcga gtgaccgcag ttatcctcgg actggcaaag gaccctctga
     7081 tgccgggata tgctacgttt gccgagacgc agaccttgac ggcgcacgca tcaatgttca
     7141 gcgccggttg gactgccagc gataagcccg acacccggtc ccgactcccc tcggcgttcg
     7201 accacggcga cagcaatcgc ggatcacaca acgccgatgt ggccttgcct atgcaagggt
     7261 ggcaccgtca gcggaaccga ccgcgactct gtcacagtcc cgatcgtgag ccgatgacca
     7321 acaatcgagt gatgtctacg gttactgttg ccccggacag cgacccgtgt gtattttgct
     7381 gatacgtcac caaattctgg tcggctgtga gaggtctacc ttgtgaagat cgttgtgatc
     7441 ggtagaggac atgtgggcgg cggtttggcc cgattgtgga gcagccgcgg ccaccaggtg
     7501 accgcgctcg gacgggacgg cggcgacgca accgacgccg aggtcgtcgt cattgccgtt
     7561 gcggcagtgc agatcccgga tgcgctcgac gccgttaccg gcctggccgg ccaaccgacc
     7621 attgatgcct gcaacatcta caccgaccgc caacacgagt ttccgtcgtt gtcacatcag
     7681 attcagtcga tcatcggcgg accgaccgcc aaggcgttct ccaccaactt cgccagtgcc
     7741 tacggccgaa tcgccgagca gcgggtggcg ccaagcaatc tgtacgccgc cgatgacgat
     7801 gcccgggaat ccgccgaaca gctcagcatc gacgccggct accaacccgt gtatgtgggc
     7861 ccactggacc caggtgcgcg cctgctcgaa gacagttccg gactgacgcg agcattggcc
     7921 gcgcagcttg ggcccttctt ctaccgatac gcacagccgg ggcaattgtg atgcgggcgg
     7981 tcaagttcac ccgatacggc gatcccgacg tgctcgaggt cgtcgaggtc ccctaccctg
     8041 ccgccggtcc cgggcaggtc gtggtggctg tggtgaccgc ggccaccaac ccgggcgaaa
     8101 tcggtattcg agaaggtcgc ttcgccgaca tctggcccgc ccatttcccc gagggacagg
     8161 gcaacgactt cgcagggtat gtcgctgaga tgggttccgg tgtcgacgat ttcgcggtcg
     8221 gagatgaggt tctcggcttc gccccgcgcg cagcacaggc ggaatacgtg gcgctcaacg
     8281 tcgatcgggt cactgccaaa ccggcggggc tttcatggga ggcggccgcg acgatcgcgg
     8341 gtgcgggggc aactgcgtgg gcgtcggtca ccgcggtcaa tccgcaaccc ggtgaaacca
     8401 tcgtggtgtc cgccgcggcc ggcggcgttg ggatctacgc cgcccagctg gcccggctgc
     8461 gcggcgccga tgtcatcggc acatgcggtc cagccaacgt cgacctattg acctcgctgg
     8521 gcatccggcc tgcgcagtac ggggagggat tgttccaact acttcgcgac ctcgcgcccg
     8581 atggcatcga cggctacatc gataccttcg gatcgggcaa cgtctcgctc gcgatcagtc
     8641 tcggcgtaca accacaccgc atcaacacgc tcatcgacca ggaggcggtg cagcgcttcg
     8701 gagtgcactc cgaagcccag gaacaggccg acacaccggg cgtctgggcc gaactcgcag
     8761 acctggtagc ccgaaagtcc atcaccgtac cgatcgcggc ggtctacgac ttcagcaccg
     8821 agcaagtccg ccaggcctac cacgacgtcg gtgcccgcca cgtcagtggc aaacgcgttc
     8881 tgcgtatcat gccggcagac gtacaagcct gtcacacgtg acgttcaatg ccgttgcggc
     8941 gcttggcccg actgcggttg ggcagccgat caccgtgatc accttcgtcc gaaggttgtt
     9001 cggcagtgta ctgacggatc actgcaagca tccggttcag cgcctcgccc agcgcgtcca
     9061 gctcatgatc ggacaacggg tcgaaaaaca gctgacgcac cagcgctacg tgattggggg
     9121 cggccttctc gatggccgtc ctgcccgcgg gcgtgagacc aaccaccgat ccccgagcgt
     9181 cctcttcgca gaactcgcga aaaacgaggc ctcgcttgac cattcgggtg acttggtgcg
     9241 atgtgcggct gcgatcccac cccagcgcga taccgagatc ctttgtccgc aattgtccgt
     9301 tcggggcctc cgacaacggc accattacgg cgtagtctgc ggccgacatg ccttgttccc
     9361 tggacagctg aagttggaac gccccttgca gctctcgtcg cacatctcgg tacgactgcc
     9421 agatgcgcgc ttcccgttta gtgagccacc cagaccttct caccacgaca cccaagctag
     9481 cagggtgttc tccccatgag tgtcgacaga aaggcaggca gcaagtgacc cacccagatg
     9541 cagaagcttt gaaagcggtg gtccgccgca acaccgaaca agtccagggt caaggcgatt
     9601 tcgccctgtt tgatgaattg ttcgccgacg acttcgtcga ccacaccccg cagcccggca
     9661 ccacagccga caaagccggt gttcgtgtcc tctacaagcg gctgcgcgac gcatttcccg
     9721 acttccgccc cgagatccat tggcagaccg tcgacggcga catcgtcacc acgtacaaga
     9781 tctaccacgg gacccacctc ggcgactttc tcggcatcgc cgccaccggc aaggccatcc
     9841 acttcgaaac ggtcgacgcg atgcgggtcc gcgacgggac gatcaccgac cactggggag
     9901 tggcgaacct gtactccgtg ctgcaacagc tcgggcagct accgacctga ccgcgtgtac
     9961 ggctcgctgt gccggtcacc ctggtgccaa ttggccagga gtcgcagggc cgtctcatcg
    10021 gccgatccgg gttcggtggt gtagacgatc aggacctggt cggggtcgcc ggatgggttg
    10081 agcgcctggt atttgacggt gagatcgccc acgagcggat ggtgataagc cttcgttccc
    10141 gtggtgcggc gttccacatt gtggttggac caccaggaac gaaactccac cgactggatg
    10201 gacaactcgc cgaccaaagc cgacagcttc tcgtcgtcgg ggtagcgtcc ggcgtccagg
    10261 cgcagcatgg caacggtgtc cgcggccacc tcgttccagt cggcatagag ctcacgggcg
    10321 tgtgggtcga agaacacgaa ccgtgcctga ttgcgcagcg gggcagggag ttcacggaac
    10381 tcggtgatga gcgcgccagc caatcggttg tgcgcaagta cgtcgagcct tcggccgagc
    10441 acgaaggcgg gcgaccacgc ttggtctaag agctccagca gtcgcagtgt cgccggatcc
    10501 accgactgtg gccgggaccg ccgtttgcgt tgcgcaggtt gcggcttggc cagtaggtgc
    10561 aggtgctggc gttcggcgtc gttgagttgc agggcggcgg ccaaggcgtc gagcacatcg
    10621 gtggaggcgc tgcggctgcg gccctgctcg agtcgggtgt agtagtcgac gctgactccg
    10681 gcgagcctgg ccagttcctc gcgccgcaga cccggcacgc gccggcgcgc tgcctgccct
    10741 tccagccccg cgtcctgcgg ggttagccgg gcccgccgca cgctcaggaa ttccgccagc
    10801 tcagacggcc ttcggtcgct catatcagtc agtctgccgt gccgcggtcg cggcgtcgcc
    10861 gcttatggtg gcccatcttg tcccccgata ggcaaggcca gcttgacccc gcaataccga
    10921 ccttcggagc tgttgtctcg gatgcacaca tccaaccact ctcgaaggga cgaacctcat
    10981 gtccacagtc atcacagggg ccccgctggc ggggcgcgtc gccatcgtca caggggcctc
    11041 cagcggcatc ggggaggcca cggccttccg gttggccacc ctcggcgcca aggttgtcgt
    11101 cgcagcgcgt cgcaaggaca acctcgacgc tctggtggct cgtatcaccg ctgccggcgg
    11161 caccgcactc gccttgcccc tcgatgtcac cgacaggtcg gccg
//
LOCUS       DASRJA010000062        16706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3329395,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000062 DASRJA010000000
VERSION     DASRJA010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16706
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3329395"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..628
                     /locus_tag="VFC01_02780"
     CDS             188..628
                     /locus_tag="VFC01_02780"
                     /inference="COORDINATES: protein motif:HMM:NF042550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6636 domain-containing protein"
                     /protein_id="HZN78602.1"
                     /translation="MKTSTKLTLITAATIAATIALPATAQAGVPPSFQTPSGNIMCWI
                     AENAATCGIVDYTYSERQRPSDCSSDEWPSTFWLSEGEPASVNCSDSPPGTREGLRTH
                     TTLDYGQTKSVGVMTCASEVSGVTCTDTSTGHFFRVSRDSYELG"
     gene            637..1545
                     /locus_tag="VFC01_02785"
     CDS             637..1545
                     /locus_tag="VFC01_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HZN78603.1"
                     /translation="MKSRIFVSLVVTVGIAAAAVSTAGCSVNSQGTPLPVPAPTATST
                     QSPRATTYSPQVELPFGDYINHMSGLAVDAAGNAYVLDLYYGQVWKQSPGATHLTTLP
                     FTDLGRPVEVAVDAEGALYVVDGLNNRVLKLVPGSTEPIVLPFNDIRPGKVAVDAAGT
                     LYVTDFGNNRVMKLADGASTPTVLPFTGIRPGSVAADTAGNVYVTDLGADRVLKLAAD
                     ASSPTVLPFNDLEIPFGVAADPAGNIYVTDFGTDRVLKLAVGASTPTVLPFTGLKEPT
                     AVAVDPASNVYIIGDGNFRVFKLPAQ"
     gene            1598..2929
                     /locus_tag="VFC01_02790"
     CDS             1598..2929
                     /locus_tag="VFC01_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78604.1"
                     /translation="MTTTDNTFHGKGFIMNRRLLGGVGVFAIAALMAVGCSSPTNSPA
                     AHESSNKPATSAATSAPATPAAARGVEATINEVPWSKVGPGWTLATWTPVTAHRPGEK
                     PAPGEADPETAATTLYLVDPLGNRYAITTFSGDDARLYLTDWSGDGSKALFVDSEYPE
                     PSKVISVDLHTGDRTTIPVQGYPRFTRPDGKALLVSTHFNGNEPGTLTRIDLDGNAQL
                     RYPTEDLGGAGQFSGGYLASPDGTQLVLSTANLGNALVPRSDDSLVVMGNDGKIIRTL
                     PTPMPDADCSPVRWWAPTVVLADCSAEHGSAHQLWTMPIDGEAPTALTAVNSGQGDDP
                     GFGADLGDGIAWKLPSGTFLQSAGACGTAFLSRLTPDGHTTRVDVPGMSESVFVTGAT
                     GDKLVLQGHLDCGGATSLVSYDPAANTSTVLLGPPVNGGGVAEAILFQEEG"
     gene            2933..3634
                     /locus_tag="VFC01_02795"
     CDS             2933..3634
                     /locus_tag="VFC01_02795"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF043047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008126 - acetylesterase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HZN78605.1"
                     /translation="MNRIARQIITLSVAAVITAATASIPAVASPPSACADLGGTVDTN
                     RICQVHTADSGYKVTFTFPVDYPDQQALVDHLTQRREQFISFVAERPPRDFPYELDAK
                     AEMFRSGTASSGTESLVFEEYSESGGAHPVTGYQAFNYDLGKGAPITLDTLFKPGTQP
                     EEVLDPIVRRKWEKFTDDYGAVDDNIVGAKVYQNFAITDDAVIFFIGQGMWLPEVAGP
                     RQVSVSRAELASVLA"
     gene            complement(3631..4770)
                     /locus_tag="VFC01_02800"
     CDS             complement(3631..4770)
                     /locus_tag="VFC01_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HZN78606.1"
                     /translation="MANSALQFGVLGPLQMSADGSDVALGAAKQRAVLALLLVNRNRP
                     VAIDSLLDAAWRGSPPPEARASLHTYVSNLRRLLSGAGADPAGALVSARPGYRLNVAD
                     RACDSGRFNIGLAAGMQAAAGGRFEQASGHLSAALAEWRGPVLDDLRDFEFVEPYAAA
                     LAEDKLVAQTLRAEAEIACGRSHSIISELEALTAEQPFRELLWAQLITAYYLTERQYD
                     ALEAYRRLKATLADELGIDPGPTLRALHERVLRQEPLEVKQIARATAKRAVATLERRT
                     RASQQSDAAAQLRKADGRTYPLRGAATRIGRLPDNDIVIDDAMVSRYHAMIVDTGASF
                     VITDLRSANGVELEDKKIRGSATLGDGDRIRISDHEFTFEFGGDG"
     gene            4863..5690
                     /locus_tag="VFC01_02805"
     CDS             4863..5690
                     /locus_tag="VFC01_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78607.1"
                     /translation="MWLIAGAAVLATVALVAGVIIGLRLGNRKTASEPPVSEALAVDF
                     TRLQTQLHAVAGIAVSAVGDGHAPMTLGQWRSGPAWSTIKVPLIIAALREEQPPKVTD
                     AMVAAITESDTAAAESIWASMGDPVTAAHKVDDVLRETGDPTTVQSQRVRPEFSAFGQ
                     TDWSLTDQVQFLSIAVCDKTNEPVFALMGEVQSDQRWGIGTLADSRFKGGWGPLPTGS
                     YLVRQMGVLVGPAGMIAVALAAQPDSGKFDDGIAALTAMARWLTDHLSALPAGHCDS"
     gene            complement(5703..7136)
                     /locus_tag="VFC01_02810"
     CDS             complement(5703..7136)
                     /locus_tag="VFC01_02810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN78608.1"
                     /translation="MDSRVGTTFGKYNVTRLLGKGGMGEVYEAYDTDKGRTVALKILA
                     HEFSDDVAFRTRFQRESQAAAILQEPHVIPIHDWGEFDGRLYIDMRLVQGQTLLDLLE
                     KGPLEPSRAVNIISQVAAALDAAHAEGLIHRDIKPQNILVTPADFAYLVDFGIAEASG
                     KTRLTTAGTQIGTLDYMAPERFGDQTSTPAVDVYSLACVLYESLTGEIPFTRDGLEIL
                     VAAHIAWPPPRPSEANPRVPVAFDDVIARGMAKEPDDRYGTAGALGRAAQRALQTVPG
                     ANAASAPGGSTVGTYAGVTVTRPSDGATTKPTAPEARDLDTGARRRGWVMPTVIAVAA
                     VAILGGIGVVIGLLASRDSGQAGPEEPTAALPPVVTGPDLSVRHQSCDQGFSLPTATG
                     FGTHAGRGTPETSCLFAESVLTSYWAAYGNASPLPRAVSAPGAVDCQTVPGALCNGAN
                     FLMHCQNEPGDSWITCVGGNNARVYLW"
     gene            7337..7684
                     /locus_tag="VFC01_02815"
     CDS             7337..7684
                     /locus_tag="VFC01_02815"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN78609.1"
                     /translation="MFTNRFTKSIASTVLAAGTLGIAAIVGAGAANADAVDDTYVASL
                     AQAGIPQIAPELEISAGHAVCRNLDEGTTTPDRVLAAFVAKHVFATREQDRAMIVASM
                     TAYCPQYLPQLHS"
     gene            complement(7695..9038)
                     /locus_tag="VFC01_02820"
     CDS             complement(7695..9038)
                     /locus_tag="VFC01_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZN78610.1"
                     /translation="MNSRSLKECYVTENPVRTYVLDTSVLLSDPWAATRFAEHEVVVP
                     LVVISELEAKRHHHELGWFARQALRMFDDLRLEHGRLDQPIPVGTQGGTLHVELNHSD
                     PTVLPAGFRTETNDARILTCAANLAAEGKRVTLVSKDIPLRVKAGAVGLAADEYHAQD
                     VITSGWTGMDEIEATSDDIDRLYADGEIDLEAARNLPCHTGIRLLGGSSHALGRVNPE
                     KRVQLVRGDREVFGLRGRSAEQRVALDLLLDESIGIVSLGGKAGTGKSALALCAGLEA
                     VLERRTHRKVVVFRPLYAVGGQDLGYLPGSESEKMGPWAQAVFDTLEGLASPAVLEEV
                     LSRGMLEVLPLTHIRGRSLHDSFVVVDEAQSLERNVLLTVLSRLGAGSRVVLTHDVAQ
                     RDNLRVGRHDGVAAVIEKLKGHPLFAHITLLRSERSPIAALVTEMLEEISPGALP"
     gene            complement(9215..10036)
                     /locus_tag="VFC01_02825"
     CDS             complement(9215..10036)
                     /locus_tag="VFC01_02825"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HZN78611.1"
                     /translation="MAQKPVPDALILELEPVVQQELRRHIDTEDLWYAHDYVPFDQGE
                     NFAFLGGNDWDPSQVTLPRTVTDALEILLIIKDNLAGYHREFVFSFILEEKWGRWIGR
                     WTAEEHLHAIALRNYLVVTREIDPAANEDVRVEHVMKGYRADTYSQVERLVFMAFFER
                     KHAVFCRNLAAQTAEPTLEGLIDRIARDEERHEEFFANLVNHLLASARDETIDAIARR
                     AGDLQVVGADIDAYQDKVAAMAEAGIFGPEQLRQVIADRITAWGLADEPRLKQYV"
     gene            complement(10089..11343)
                     /gene="glyA"
                     /locus_tag="VFC01_02830"
                     /pseudo
     CDS             complement(10089..11343)
                     /gene="glyA"
                     /locus_tag="VFC01_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177787.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
     gene            complement(11449..12912)
                     /locus_tag="VFC01_02835"
     CDS             complement(11449..12912)
                     /locus_tag="VFC01_02835"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine hydroxymethyltransferase"
                     /protein_id="HZN78612.1"
                     /translation="MTADSTATTTTAPGAEYADTASAAYRSILQIIESVEPRIAAATR
                     KELADQRDSLKLIASENYASPAALLTMGNWLSDKYAEGTIGHRFYAGCQNVDTVETVA
                     AEHARELFGSPYAYVQPHSGIDANLVAYWAILATRVEAPTLAEHGVKNVNDLSEADWE
                     KLRNKLGNQRLLGMSLDAGGHLTHGFRPNISGKMFHQRSYGTDPETGLLDYDALAKSA
                     REFKPLILVGGYSAYPRRINFAKLREIADEVGATLMVDMAHFAGLVAGKVFTGDEDPV
                     PHAHLTTTTTHKSLRGPRGGLVLATADYSDAVDKGCPMVLGGPLSHVMAAKAVALAEA
                     RQPSFQAYAQRVADNAKALAEGFLKRDARLVTGGTDNHIVLLDVTSFGLTGRQAESAL
                     LDSGVVTNRNSIPADPNGAWYTSGIRLGTPALTTRGFGPHDFDRVAELMIDVLSNTQP
                     EGTSKAKYKLADGTAERVHAGAAELLAANPLYPGLSL"
     gene            complement(12918..14117)
                     /locus_tag="VFC01_02840"
     CDS             complement(12918..14117)
                     /locus_tag="VFC01_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZN78613.1"
                     /translation="MEPGTLIREYLLLGLRFDRIEEGYVDSFTGDPALRQQVQNEPVP
                     DPADLAIQADRLATELPGGLDPARADYVSAHLRALTCAGRKFAGQDVGFVDEVHAYFD
                     VHIAKGDPETYRQAHRKLDDALPGSGTLVERMQADRASEEVPPEKLEEAIHAFSSALR
                     DRVRAEYPLPDTETITYEVVTDKPWSGFNYYLGDYRSTVAVNADLKQQMSNLPRLVAH
                     ESYPGHHTEHCRKEAGLVEGKGQAEQTIFLVNTPQCLMAEGLADLALHAVIGPGWGAW
                     AAEIYADLGLRFDGERAETVSEAAAALADVRQDAALMLHDEHRDVDDVVDFLKRWLLV
                     NDERARQMLRFLSSPLWRAYTSTYVEGYRLLRSWLEARPGGVSLTERFGRLLDEPLIP
                     SSLRDVG"
     gene            14148..15086
                     /gene="coaA"
                     /locus_tag="VFC01_02845"
     CDS             14148..15086
                     /gene="coaA"
                     /locus_tag="VFC01_02845"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HZN78614.1"
                     /translation="MPRLSEPSPYVEFDRSQWRALRMSTPMKLTEDEVLQLRGLGEKL
                     DLLEVEEVYLPLARLIHLQVAARQRLFATTAEFLGEPQQNPDRPVPFVIGVAGSVAVG
                     KSTTARVLQALLARFGNHPQVDLVTTDGFLYPNAELSRRNLMHRKGFPESYDRRALMR
                     FVTAVKSGADKVCAPVYSHLLYDIVPGEKQIIKHPDILILEGLNVLQTGPALMVSDLF
                     DFSVYVDARIEDIEQWYINRFLGLRTTAFADPASHFHHYATLTDEAAVFAARDIWHSI
                     NRPNLIENILPTRPRATLVLRKDADHSINRLRLRKL"
     gene            complement(15129..15536)
                     /locus_tag="VFC01_02850"
     CDS             complement(15129..15536)
                     /locus_tag="VFC01_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78615.1"
                     /translation="MSAITIDRTGTRDGLLRLAMRADAVLTGLAGVAGIPLAGWLAET
                     SGTTRAFEYGMAAFFIAYGVIVFGLAALPSVRRAGMAVIVANLLYTVAAVVLVLADVF
                     PLTATGVVLTLASGVYTLVFAELQYQGWRRAKA"
     gene            complement(15625..16032)
                     /locus_tag="VFC01_02855"
     CDS             complement(15625..16032)
                     /locus_tag="VFC01_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78616.1"
                     /translation="MTAINTRPVRESTDSLLRFALRADATLCAGLGLLVAMTADPLSR
                     LSGLSSTSEWIGGAALVIYGAALYMAAGLPDVRRVGVGVLAGNIAFAILVTVVLIAGW
                     LPLTEFGVVATAAFTLATLGFAYAQYLGVRRLA"
     gene            complement(16170..>16706)
                     /locus_tag="VFC01_02860"
     CDS             complement(16170..>16706)
                     /locus_tag="VFC01_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78617.1"
                     /translation="RSPGLRADRATLRRTEAALPLRSDNPLDEVDADTFARLTRRALG
                     HYGDLSKLVASPLTALPMIDERLAARGAPDQPLERANELKALLADRIARLKPRDGGDF
                     GTTEQWRHYNSLYFPYVVGVRAYAQNATAAGLDPVARQAWQWFVTEVPQRSLHNWQNA
                     AARLIAADLRGNLVTAQD"
BASE COUNT         2864 a         5523 c         5521 g         2798 t
ORIGIN      
        1 gcgtagcgcc agcgctggct gtcgggtcgt ttcggcaccc aagcagccta ccgattggtg
       61 atttcggtgc gcttacacac gctcagcaac acgctcagcg tcggtttgcg caccgaaatc
      121 gcgaggccaa ggagttgcca agaatcattt gagaaccgca cgaggcgtcc gccgcagcct
      181 ttcagacatg aaaacttcga cgaagctcac gctgatcacc gcagcgacga tcgccgcaac
      241 catcgcgctc ccggcgaccg cgcaggcggg cgtgcccccg tcatttcaaa caccgtcggg
      301 caacatcatg tgctggatcg cagagaacgc agccacctgc gggatcgtcg actacacgta
      361 ttcggaacgg caacggccat ccgattgcag ctcggatgaa tggccgagca cgttctggct
      421 ctcggagggc gagccggcgt cagtgaattg ctccgacagc ccgcccggca cgcgcgaggg
      481 cttgcgcacc catacgacgc tcgactacgg gcagacgaag tccgtcggcg tgatgacgtg
      541 tgccagtgag gtctcgggtg tgacgtgcac cgacaccagt accggccact tcttccgcgt
      601 ctcccgcgac tcctacgagc tcgggtgacc tcggccatga agtctcggat tttcgtctct
      661 cttgtggtga cggtggggat cgctgccgcg gcggtgtcga ccgcggggtg ttcggtcaat
      721 agccaaggca cgccactccc ggtgccggcg cccactgcga cgtccacgca atcaccgaga
      781 gccacgacat attcgcctca ggtcgagctg ccgttcggtg actacatcaa tcacatgtcc
      841 gggctggcgg tagacgccgc gggcaacgcg tacgtcctcg acctctacta cggccaagtg
      901 tggaagcagt cgccaggcgc gactcacctc accacgctgc cgttcactga cctcggccga
      961 cccgttgagg tggcggtcga cgcagagggc gccctctatg tcgtcgatgg actcaacaac
     1021 cgggttctga agttggtgcc cggatccacg gagccaattg tgctgccgtt caacgatatt
     1081 cgtcccggca aggtcgcggt ggatgcggca ggcaccctct acgtcaccga ctttggcaac
     1141 aaccgggtga tgaagctggc tgacggggca agcaccccga ccgtgttgcc gttcaccggc
     1201 attcgacccg gcagtgtggc ggccgatacc gcaggcaatg tctacgtcac cgacctcggc
     1261 gctgaccggg tgttgaaatt ggcggcggat gccagcagcc caaccgtgct gccgttcaac
     1321 gatttagaaa tacctttcgg cgtggcggcc gaccccgcgg gcaacatcta cgtcaccgac
     1381 ttcggcaccg accgcgtgtt gaagctggcg gtcggtgcga gtactccaac agtgctgccg
     1441 ttcaccggcc tcaaggagcc caccgctgtg gcggtcgacc ccgcgagcaa cgtctacatc
     1501 attggcgacg gcaacttccg cgttttcaag ctgcctgcgc agtgatgacg ccaagattca
     1561 cttgagaaac cgtcgagcca gcaacagcaa cctagtcatg acaacaaccg acaacacgtt
     1621 ccacggaaag ggcttcatta tgaaccgcag attgctcggc ggggtaggcg tcttcgcgat
     1681 cgccgccctg atggcggtgg gctgctcatc gccgaccaat tcccctgcgg cacacgagtc
     1741 atcgaacaaa ccggccacat ccgcggcgac gtccgcaccc gcgacccctg ccgcggctcg
     1801 cggtgtcgag gccaccatca acgaggtgcc atggtcgaag gtcggcccgg gctggacgct
     1861 cgccacctgg acacccgtca ccgcccaccg accgggcgaa aaaccggccc cgggcgaggc
     1921 ggaccccgag acggctgcca cgacgctgta cctcgtcgac ccgttgggta accgctatgc
     1981 aatcaccacc ttctcgggtg atgacgcgcg gctctatttg accgactggt caggcgacgg
     2041 gagcaaggcc ctattcgtgg actcggaata ccccgagccc tccaaggtca tctcggtcga
     2101 cctgcacacg ggcgaccgga ccacgattcc cgtccagggc tatccccgct tcacgcggcc
     2161 cgacggcaag gcactgttgg tttccacaca cttcaacggc aatgagccag gaacgctgac
     2221 gcggatcgac ctcgacggca acgcgcagct gcgctacccg accgaggatc tcggcggcgc
     2281 gggccagttc agcggtggct atcttgcgtc gcccgacggg acgcagctcg ttctcagcac
     2341 cgccaacctc ggaaacgcgc ttgtgccgcg cagcgacgac agcctggtgg tgatgggcaa
     2401 cgacggaaag atcattcgca cactgccgac accgatgccc gacgccgact gctctccggt
     2461 gcggtggtgg gcacccaccg tggtcctcgc cgactgcagt gcggagcacg gctcggcaca
     2521 ccagctttgg acaatgccta tcgacggtga ggcgcccacc gcgctcacgg ccgtcaactc
     2581 aggccagggc gatgatcccg ggttcggcgc ggatctcggc gatgggatcg cctggaagct
     2641 gccgagcggc acgttccttc agtccgcggg agcctgcggc accgcgttcc tgtctcgctt
     2701 gaccccagac ggccacacca cacgcgtgga tgttcccggc atgtccgaga gcgtgttcgt
     2761 caccggggcg accggtgaca agttggtgct gcagggccac ttggactgtg gcggtgccac
     2821 gtctctggtg agctacgacc cggcggccaa cacctccact gtgctgcttg gcccgcccgt
     2881 caacggcggc ggcgtcgcag aggcgatcct gttccaggaa gagggctagg ccatgaatcg
     2941 cattgcgcgc cagatcatca cgctcagcgt cgccgcggtc atcaccgccg cgacggcctc
     3001 tatccctgct gtggcgagcc cgccctcggc gtgcgccgat ctcggcggaa ccgtcgacac
     3061 caaccggatc tgccaggtcc acaccgccga ctcgggttac aaggtcacct tcaccttccc
     3121 ggtcgactac cccgaccagc aggcgctcgt cgaccacctg acgcagcggc gagagcaatt
     3181 catcagcttc gttgctgagc ggcccccgcg tgactttccg tatgagctcg acgccaaagc
     3241 ggaaatgttc cgatccggaa cggcgagctc gggcaccgaa agcctggtgt tcgaggaata
     3301 cagcgaatca gggggcgccc atccggtaac cggctaccag gccttcaatt acgacctggg
     3361 caagggcgcc ccgatcaccc tcgacacgtt gttcaaaccg ggcactcaac cggaggaggt
     3421 gttggacccg attgtccgcc gtaaatggga gaagttcacg gacgactacg gcgcggtgga
     3481 cgacaacatc gtcggcgcca aggtctacca gaatttcgcc atcactgacg acgcggtgat
     3541 cttcttcatc ggccagggca tgtggctgcc ggaagttgct gggccacggc aagtctcggt
     3601 gtcccgcgct gagctggcgt cagtgctggc ttagccgtcg cccccaaact caaaggtgaa
     3661 ttcatggtcg ctgatgcgga tgcggtcgcc atcgcccagc gtcgcgctgc cacgaatctt
     3721 cttgtcctcc agctcgaccc cgttggctga tcgaaggtcg gtgatgacaa agcttgcacc
     3781 ggtgtcgacg atcatcgcat ggtaacggct gaccatggca tcgtcgatga cgatgtcgtt
     3841 gtcggggagg cgcccgattc gggtggcggc tcctcgcagc ggataggtac gcccgtcggc
     3901 cttgcgtagt tgggcggcgg catcagactg ctgggaggcc ctggtgcgcc gttcgagcgt
     3961 cgcaacagca cgtttcgccg tcgcacgggc tatctgtttg acttccagcg gctcttggcg
     4021 aaggacccgc tcgtgcaggg cccgcaacgt tggccccggg tcgatgccca attcgtctgc
     4081 gagcgtagcc ttcaaccgcc gatacgcctc gagcgcatcg tattgccgct cggtcaggta
     4141 gtacgcggtg atcaactggg cccacaacag ctcgcggaag ggctgctcgg cggtaagggc
     4201 ttcaagctca gagataatgg aatgacttct gccgcacgct atctcggctt ccgctcgcaa
     4261 cgtctgtgcc accagtttgt cctccgccaa tgcggcggcg taaggctcaa cgaactcgaa
     4321 gtcgcgcaga tcgtcgagga ccggcccgcg ccactcggct aacgctgccg acagatggcc
     4381 gctggcctgt tcgaaccggc cgcccgccgc ggcctgcatt ccggctgcga gtccgatgtt
     4441 gaagcggccg ctatcgcacg cccggtcggc gacgttcagt cgataccctg gccgcgcgct
     4501 gaccagagcc ccggctggat cggcacctgc cccggacaac agtcggcgaa ggttggagac
     4561 gtaggtatgc aggctggctc gagcctccgg cggtgggctg cctcgccatg ccgcgtccaa
     4621 caatgaatcg attgcgacgg ggcggtttcg attgaccagc aacagggcca gcaccgctcg
     4681 ctgtttagcc gcacccaagg ccacgtcgga gccgtcggcg ctcatctgca acggtccgag
     4741 caccccgaac tggagagcgc tgttggccat cgcggcagca accctttcgg ttcggcggtg
     4801 cgtcgatacc cattgtggcc cgtcccgtag tctcacggac agcaatgcgt aaccgagggc
     4861 gtgtgtggct catcgccggt gccgctgtgc tcgcaacggt ggcgctggta gcgggcgtga
     4921 tcatcgggct ccgtctcggc aatcgaaaga cggcatcaga gccgccggtg tcggaagcgt
     4981 tggcggtcga cttcacccgc ctgcagacac agctgcacgc ggtggcgggc atcgcggtca
     5041 gcgccgtggg cgacgggcat gccccgatga cgctgggaca gtggcgaagt gggcccgcct
     5101 ggtcgacgat caaggttccg ctgatcatcg cggcgcttcg tgaggagcag ccgcccaagg
     5161 tgaccgacgc gatggtagcc gcaatcaccg aatctgacac cgcggccgcc gaatcgatct
     5221 gggcgagcat gggtgatccc gtcacagccg cgcacaaggt cgatgacgtg ctgagagaaa
     5281 ccggtgatcc gacgacggtg cagtcgcaac gagtacgccc agagttctcc gcgttcggcc
     5341 agaccgactg gtcgctgacc gatcaggtgc agttcctgtc aattgccgtg tgtgacaaga
     5401 caaatgaacc ggtcttcgca ctgatgggag aggttcaatc cgaccagagg tggggtatcg
     5461 gcacgcttgc ggacagccga ttcaaaggcg gctgggggcc gctacccacc ggaagctatc
     5521 tggttcggca gatgggcgtg ctggtcggtc cggcaggaat gattgccgtc gcattggccg
     5581 cacagcccga ctcagggaag ttcgacgacg ggatcgccgc gctcacagcg atggccaggt
     5641 ggctgacgga tcacttgtcg gcgctgccgg ccggccactg cgattcctga ctgtcgaagc
     5701 actcaccaca agtagacacg cgcgttgttt ccgccgacgc acgtgatcca gctgtctccc
     5761 ggctcgttct ggcagtgcat gaggaagttc gcgccgttgc acaaggcgcc gggcacggtc
     5821 tggcaatcaa cagcgcccgg cgccgacacc gccctcggca gcgggctggc attgccgtaa
     5881 gcggcccaat acgacgtcag cacactctcc gcgaaaagac acgaggtttc gggtgtcccg
     5941 cgtccggcgt gagtgccgaa gccggtggcc gtcggcaacg aaaagccttg atcgcaggac
     6001 tgatgccgga cgctgagatc cggtcccgtg accaccgggg gtagcgcagc ggtgggctcc
     6061 tccggcccgg cctgcccgga gtcgcgactg gcaagtaggc cgatcaccac accgatgccg
     6121 cccagaatcg ccaccgccgc cacggcaatc acggtcggca tcacccatcc ccgacggcgc
     6181 gcgccggtgt cgaggtcacg agcctccggt gccgtgggct tggttgtcgc gccgtcggat
     6241 ggtcgggtaa cggtgactcc cgcgtatgtc cccacggtgg agccaccagg cgctgacgcg
     6301 gcgttggcgc cggggacggt ctgcagggcg cgctgcgccg cgcggcccag agcgcctgcc
     6361 gttccgtacc ggtcgtcggg ctccttggcc atgccgcgag caattacatc gtcgaaggcg
     6421 accggaacgc gaggattggc ctcgctcggc cgcggcggtg gccaggctat atgggccgcg
     6481 accaaaattt ctaaaccgtc gcgcgtaaac gggatttcac cagtcagaga ctcgtacaac
     6541 acacatgcca gcgagtagac atcgaccgcg ggcgtactgg tttgatctcc aaatcgttct
     6601 ggcgccatgt aatccaacgt gccgatctga gttccagccg tcgttagccg cgttttgccg
     6661 ctggcctcgg cgattccgaa gtcgaccagg tacgcgaaat cggcaggggt gacaaggatg
     6721 ttctgcggct tgatatcgcg atggatcaag ccctcggcgt gggcggcatc cagggcagcg
     6781 gcgacctgac tgatgatgtt gaccgcgcgg gacggttcca gcgggccctt ctcgagcagg
     6841 tcaagcagcg tctggccctg caccaggcgc atgtcgatgt agagtctgcc gtcgaactca
     6901 ccccaatcgt gaatcggaat gacgtgcggc tcttgcagaa tcgccgccgc ctgggactcg
     6961 cgctgaaacc gcgtgcgaaa tgcgacgtcg tccgaaaact cgtgggcgag tatcttcagt
     7021 gcgacggttc ggcccttgtc ggtgtcgtag gcctcgtaca cctcacccat cccgcccttg
     7081 ccgagcagcc gcgtgacgtt gtacttgccg aacgtcgttc cgacgcgcga gtccaccgca
     7141 cttcctttcg tttcctgaga tttccatggc atacgcacgc agtatctccc ccgcggccga
     7201 accgcgcgac ggtttaccgg ccagaagccg atcacaagaa gtcgccaaga atcgcttgag
     7261 agtcgcgcga ggtgtccacc gcagccttga catgcccggc aacacggccg gcgctaccgg
     7321 ataggacccc aacacgatgt tcaccaaccg cttcaccaag tcaatcgcga gcactgtgct
     7381 cgcagccgga acgctcggga tcgccgcgat cgtcggcgcc ggcgcggcca acgccgacgc
     7441 tgttgacgac acctacgtgg ccagcctggc ccaggcgggc atcccgcaga tcgcaccgga
     7501 gcttgaaatc tcggcaggcc atgccgtgtg ccgaaacctc gacgagggca ctactacccc
     7561 cgaccgggtg ctcgcggcgt tcgtcgcgaa gcacgtattc gccaccagag agcaggacag
     7621 ggcgatgatc gtcgcatcga tgacggccta ctgcccgcag tacctgccac agctgcactc
     7681 atagccgaga ttgctcaggg caacgcgccg gggctgatct cctcgagcat ctcggtcacc
     7741 agcgccgcga tgggcgagcg ctcgctgcgc agcagcgtga tgtgggcgaa cagcgggtgg
     7801 cccttgagct tctcgatcac cgccgccaca ccgtcgtggc ggccgacgcg gaggttgtcg
     7861 cgctgggcga catcgtgggt gagcaccacc cgcgaaccgg cgcccagccg ggacagcacg
     7921 gtcagcagca cgttgcgttc cagcgattgc gcctcgtcga cgacgacgaa ggagtcgtgc
     7981 agcgagcggc cgcggatatg ggtgagcggc agcacctcga gcatgccgcg ggatagcact
     8041 tcttccagta ccgcggggct ggccagacct tcgagcgtgt cgaacacggc ctgcgcccac
     8101 gggcccatct tttcgctctc gctgccgggc aggtagccga ggtcctgtcc gccgaccgcg
     8161 tacagcggcc ggaatacgac gaccttgcga tgggtgcgac gctccagcac cgcctccagg
     8221 cccgcgcaca gcgcgagcgc cgacttaccg gtcccggcct tgccgcccaa cgagacgatg
     8281 ccgatcgact cgtcgagcag cagatccagc gccacccgct gttcggcgga gcggccgcgc
     8341 aacccgaaca cctcgcgatc gccgcgcacc agctgcacgc gcttttccgg gttgacccgg
     8401 ccgagggcgt gggaactgcc gccgagcagc cgaataccgg tgtggcacgg caggtttcgc
     8461 gccgcttcca ggtcgatctc gccgtcggcg tacagcctgt cgatgtcgtc ggacgtagcc
     8521 tcgatctcgt ccatcccggt ccagccggac gtgatgacgt cctgggcgtg gtactcgtcg
     8581 gcggccagac cgacggcacc cgccttcacc cgcagcggaa tgtccttgct gaccaacgtg
     8641 actcgcttgc cttccgcggc gaggttggcc gcgcaggtga ggatccgcgc gtcgttcgtc
     8701 tcggtgcgga agcccgcggg aagcaccgtc ggatcgctgt ggttgagctc gacatgcagc
     8761 gtgccgcctt gcgtcccaac gggaatcggc tgatccagcc gtccatgctc cagccgcaga
     8821 tcgtcgaaca tccgcagggc ctgccgggcg aaccagccaa gctcgtggtg atgccgtttg
     8881 gcctccagct cgctgatgac caccaacggg accacgactt cgtgttcggc aaacctcgtg
     8941 gccgcccagg gatcggacag cagcacggaa gtgtcgagca cataagtccg gacaggattt
     9001 tcggtcacgt agcactcctt gaggctgcgc gagttcatgc ggccccgcac gaacttctcg
     9061 gcggacactg cggtaccagg accggggccg gtcctctcat gatcgaaatc atcgggaccg
     9121 cccggatagc agagcatgtc gctagccatc gagaaacgac gctactctcg gtgagcgctt
     9181 cgcgccgtgc agacgcgccg tggctaacta ggcgtcacac gtactgcttc agccgaggct
     9241 cgtcggccag accccacgcg gtgatgcgat cggcgatcac ctggcgcagc tgttccggtc
     9301 cgaagatgcc cgcctcggcc atggcagcca ccttgtcctg gtaagcgtcg atgtcggcgc
     9361 ccaccacttg aaggtcgccg gcgcggcgtg cgatcgcatc gatcgtctcg tcgcgggcgg
     9421 acgcgagcag gtggttgacg aggttggcga agaactcctc gtggcgctct tcatcgcggg
     9481 caatccggtc gatcaagccc tccagcgtcg gctcggcggt ctgcgctgcg aggttgcggc
     9541 agaagacggc gtgcttgcgc tcgaagaaag ccatgaacac cagccgctcg acctggctgt
     9601 acgtgtcggc gcggtagccc ttcatcacgt gctcgacgcg cacgtcctcg ttggcggccg
     9661 ggtcgatctc gcgggtgacc acaaggtagt tgcgcagtgc gatcgcgtgc aggtgctcct
     9721 cggcggtcca gcggccgatc cagcggcccc acttctcctc gaggatgaag ctgaacacga
     9781 actcgcggtg gtagccggcg aggttgtcct tgatgatcag caggatctcc agggcgtcgg
     9841 tgacggtcct gggcagcgtc acctgcgacg ggtcccagtc gttgccgccg aggaaggcga
     9901 agttctcgcc ctggtcgaac ggcacgtagt cgtgggcgta ccagaggtct tcggtgtcga
     9961 tgtggcgacg cagctcctgc tgcacgaccg gctcgagctc gagaatcagc gcatcaggta
    10021 caggtttctg tgccatgaaa gtaactgtaa cccgagttca cacattttct gaaattgggt
    10081 aaggcgtgtc agacgcggcc gtgcagctcc cactcttcta ggccgtcgta cagcggaaac
    10141 tcgcgggcca ggcggcgcac ccgagcgccc agcgccgaca cgtcggccgc ttgcgtcaac
    10201 gccatcgcga tgacatcggc aacctcggcg aactccacgt cgccgaagcc gcgggtggcc
    10261 aacgcggacg tgccgatccg caggcccgac gtgaccatcg gtggccgcgg atcgttcggc
    10321 acggcgttgc ggttgaccgt gatgccgatg tcacccagca gatcctcggc ggccttgccg
    10381 tccagcgggg aatcgcgaag atcggcgagc accaggtgga cgtccgtgcc gccggtgacg
    10441 acgctgacgc ccgcccgttt gacgtcggcg cccagcagtc ggtcggcgat gacgcgggcg
    10501 cccgaaagct tgcggcgttg ccggtcggcg aattccggcg tcgccgcgat cctgaacgtc
    10561 accgcctttg ccgcgaccac ttgcatcgcc ggaccgcctt gcgtgcccgc aaacaccgcc
    10621 gagttgacgg ccttcgcgaa ttcccgcctg cccaggatca atccggaacg ggcgccgccc
    10681 aacgtcttgt gcacagtcgt cgacaccaga tcggcgtgcg ggaccggcga cggatgcagg
    10741 tccgcggcca ccagaccagc gaagtgcgcc atgtccaccc acaggtgcgc cccgacctcg
    10801 tcggcgatgt cgcggaatgc cgcaaagtcg agcgtgcgcg gatacgccga ccaccccgcg
    10861 accagaaccc cgggccgaaa ctggatggcc ttggcccgca cggcgtccat gtcgatgagt
    10921 ccggttacgg aatcgactcc atagcaaccg gattcgtaga ttctcccgga gaagttcagc
    10981 ttcatgccat gcgtcaggtg accgccgttg gcgaggtcga gaccgagcag ccgctccccc
    11041 ggcgacatca gcgccgctag cacggcagcg ttggcctggg cgccggaatg cggttgcaca
    11101 ttaacgaatt cggcgttgaa caaggccttg gcacggtcgc gggcgatgtt ctcgatggcg
    11161 tcgacgtgct tgcatccgcc gtagtaccgg cgacccggca gtccctcggc gtacttgttg
    11221 gtgagcacgc tgccctgcaa ctgcagcacc gagcgcggtg cgaagttctc cgacgcgatc
    11281 atctccagca tgtcgcgctg gcggcccagc tccttgtcca acgtcggcca gcggggcgga
    11341 catcgcggac gtggcaaggt cagctgtcgc ggtcacggcg gcgagtctgg catacccgca
    11401 cggggtatgg gggcctaccc tggacggccg ctgagagatc gctgggaact acagcgacaa
    11461 acccggatac aacgggttgg ctgccagcag ttcggcggcg cccgcatgca cacgttcggc
    11521 ggtgccgtcg gccagcttgt acttggcctt cgaggtgccc tccggttgcg tgttggacag
    11581 cacgtcgatc atcaactcgg cgacgcggtc gaagtcgtgc ggcccgaacc cgcgggtggt
    11641 tagcgccggg gtgcccaacc ggatgccgct ggtgtaccag gcgccgttgg ggtcggccgg
    11701 gatcgagttg cggttggtga cgacgccgga gtccagcagc gccgattcgg cctgcctgcc
    11761 agtgagcccg aacgacgtca cgtcgagcag cacgatgtgg ttgtcggtgc cgccggtgac
    11821 caggcgcgcg tcgcgcttga ggaagccctc ggctagtgcc ttggcgttgt cggcgacgcg
    11881 ctgcgcgtag gcctggaagc tgggctggcg cgcctcggcg agcgcgacgg ccttggccgc
    11941 catcacgtga gaaagcggtc cccccaacac cattgggcag cccttgtcga ccgcgtcgga
    12001 gtagtcggcg gtcgccagca ccagaccgcc gcgcggccct cgcagcgact tgtgtgtggt
    12061 ggtcgtggtg aggtgggcgt gcggcacggg gtcctcgtcg ccggtgaaca ccttgcccgc
    12121 gaccagaccg gcgaagtgcg ccatgtcgac catcagggtc gcgcccacct cgtcggcgat
    12181 ctcgcgcagc ttggcgaaat tgatcctgcg cgggtaggcc gagtagccgc cgacgaggat
    12241 cagcggcttg aactcacgcg cggacttggc gagcgcgtcg tagtcgagca gaccggtctc
    12301 cgggtcggtg ccgtagctgc gctggtggaa catcttgccc gagatgttgg gccggaagcc
    12361 gtgggtgagg tgcccgcccg cgtccagcga catgccgagc agccgctggt ttccgagctt
    12421 gttgcgcagc ttctcccaat ccgcttccga gaggtcgttg acgttcttga cgccgtgttc
    12481 ggccagcgtg ggcgcctcga cacgggtggc caggatcgcc cagtacgcaa cgaggttggc
    12541 gtcgataccc gagtgcggct gcacgtaggc gtagggcgag ccgaacagct cgcgggcgtg
    12601 ttcggcggcg acggtctcga cggtgtcgac gttctggcag ccggcgtaaa accggtgtcc
    12661 gatggtgcct tccgcgtact tgtcggacag ccagttaccc atcgtcagca acgccgcggg
    12721 ggacgcgtaa ttctcgctgg cgatcagctt gagcgaatca cgttgatcgg caagctcttt
    12781 gcgggtcgcc gcggctatgc ggggctcgac ggattcgatg atctgcagga tcgagcggta
    12841 ggcggcgctc gcggtatcgg cgtactcggc accgggagcg gtggtggtgg tcgcggtcga
    12901 gtctgcggtc atgcagttca gcctacgtcc cgcagcgacg acgggatcag cggctcgtcg
    12961 agcagccgac cgaaccgttc ggtgaggctg acgccgccgg gcctggcctc tagccagctc
    13021 cgcagcaacc ggtagccctc gacgtaggtg ctggtgtagg cgcgccacag cggcgaggac
    13081 aagaagcgca gcatctgtcg ggcgcgctcg tcgttgacca gcagccagcg cttgaggaag
    13141 tcgaccacgt cgtcgacgtc gcggtgctcg tcgtgcagca tcagcgcggc gtcctgtcgc
    13201 acgtcggcca gcgccgcagc cgcttccgac accgtctcgg cgcgttcgcc gtcgaaccgc
    13261 agcccgaggt ccgcatagat ttcggcggcc caggctcccc agccgggccc gatcacggcg
    13321 tgcagcgcca ggtcggccag accctcggcc atcaggcact gtggcgtgtt caccaggaag
    13381 atcgtctgct cggcctggcc cttgccctcg accagcccgg cttccttacg acagtgctcg
    13441 gtgtggtggc cgggatacga ctcgtgtgcg acgagccgag gcaggttgga catctgctgc
    13501 ttgagatcgg cgttgacagc gaccgtggag cggtagtcac cgaggtagta attgaacccc
    13561 gaccacggct tgtcggtcac cacttcgtag gtgatggtct cggtgtcggg cagcgggtac
    13621 tcggcccgca cccggtcgcg cagcgcgctg gagaacgcgt ggatggcttc ctcgagcttc
    13681 tcgggcggca cctcttcgga cgcgcggtcg gcctgcatgc gctcgacgag cgttccgctg
    13741 ccgggtaatg cgtcatcgag cttgcggtgg gcctgccggt acgtttcggg atcgcctttg
    13801 gcgatgtgaa cgtcgaaata ggcgtgcacc tcgtcgacga agccgacgtc ctggcctgcg
    13861 aacttccggc ctgcgcacgt cagcgcgcgc aggtgggcgc tgacatagtc ggcgcgggcg
    13921 ggatcgaggc caccgggcag ttcggtggcc agccggtcgg cctggatggc caggtcggcg
    13981 ggatcgggca cgggctcgtt ctgcacctgc tggcgcaggg cggggtctcc ggtgaaggag
    14041 tcgacgtagc cttcctcgat ccggtcgaag cgcaaaccga gcagcagata ctcgcggatg
    14101 agggtgccgg gctccatggc gaccacgcta actgcaagac tgagcgcatg ccgcggctga
    14161 gcgagccgag cccctacgtg gagttcgacc ggagtcaatg gcgcgcgctg cgcatgtcga
    14221 cgccgatgaa gctgaccgaa gatgaggtgc tacagctgcg gggtcttggc gagaagctcg
    14281 acctgctcga ggtcgaagag gtctacctgc cgctggcccg gttgatccac cttcaggtcg
    14341 cagcccgtca gcggttgttc gccaccaccg ccgagttcct cggtgagcca cagcagaatc
    14401 cggaccggcc ggtgccgttc gtcatcggcg tcgcgggaag cgtcgccgtc ggcaagtcca
    14461 cgaccgcccg tgtgctgcag gctctgctgg cccgcttcgg aaaccacccg caggtcgacc
    14521 tggtcaccac cgacggtttc ctctacccca acgccgagct gagccgtcga aacctgatgc
    14581 accgcaaagg ttttccggag agctacgatc ggcgcgcgct gatgcggttc gtcacggcag
    14641 tgaagtcggg cgctgacaaa gtctgtgcgc ccgtgtactc gcacctgctc tacgacatcg
    14701 tgccgggcga aaagcagatc atcaagcatc cagacatcct gattctggag ggcctcaacg
    14761 tattacagac gggtccggcg ctgatggtgt cggacctgtt cgacttctcg gtgtacgtcg
    14821 acgcccgcat cgaggacatc gagcagtggt acatcaaccg gttcctcggc cttcgcacga
    14881 ccgcattcgc cgatcccgcg tcgcacttcc accactacgc cacgctgaca gatgaggccg
    14941 cggtgttcgc cgcccgcgac atctggcatt cgatcaaccg gcccaacctg attgagaaca
    15001 tcctgccgac gcggccacgc gcgacgctgg tgttgcgcaa ggacgccgac cactccatca
    15061 accggttgcg gctgcgcaag ctctaaaccc gcccgactgt cggccgagcg ggcttgagcg
    15121 gcaacggctt acgccttggc gcgacgccag ccctggtact gcagctcggc gaacaccagc
    15181 gtgtagacgc ctgacgccaa agtcagcacg acgcccgtgg cggtcaacgg gaagacgtct
    15241 gcgaggacca gaacgaccgc ggcgacggtg tacagcaggt tggcgacgat cacggccatg
    15301 ccggcccggc gcaccgacgg caatgcggcg agcccgaaca cgatcacgcc gtacgcgatg
    15361 aagaacgcgg ccatgccgta ctcgaacgct ctggtggtgc ccgatgtctc cgcgagccaa
    15421 ccggccagcg ggatgccggc cacaccagcg aggccggtga ggacggcgtc ggcacgcatc
    15481 gccagtcgaa gcaacccgtc gcgggtgccg gtgcggtcaa ttgtgatggc ggacatggtt
    15541 ttcctttctc ggtgggtggt taccggttcc ggagccgacg ccgcaacctc tgacgacggc
    15601 ggctccggac cagtcatgag ggagtcacgc aagacggcgc actccgaggt actgggcgta
    15661 ggcgaagccg agggtggcga gggtgaacgc ggcggtggcg accacgccga attcggtcag
    15721 cggtagccaa ccggcgatga gcacaacggt gacgaggatc gcgaacgcga tgttcccggc
    15781 gagcacaccg acgccgacgc gtcggacgtc cggcagaccg gcggccatgt acagcgccgc
    15841 gccgtagatc accaacgcgg cgccgccgat ccactccgag gtcgacgaca gaccggacag
    15901 gcgcgacagc ggatcggccg tcatcgcgac gagcagcccc aggccggcgc acagtgtggc
    15961 gtcggcgcga agggcgaagc gcagtagtga gtcggtactt tcccggaccg ggcgggtgtt
    16021 gattgcggtc atgacactga gattgctcag aacccactgc cagatcgacg ccagaagctg
    16081 ccagctactg ccaaccgcag gtcagcgacg atgcgggcga ggaacgagcc cgagaaggag
    16141 ctgaccaatt gggcagagcc agagcgtcct taatcttggg ctgtcaccag attgccgcgc
    16201 agatcggcgg cgatcagccg ggccgccgcg ttctgccagt tgtgcagcga ccgttgcggc
    16261 acctcggtga cgaaccactg ccaggcctga cgcgcgaccg ggtccaggcc ggcggcggtg
    16321 gcgttctgcg cgtaggcgcg tacgccgacg acgtacggga agtacagcga gttgtagtgg
    16381 cgccactgct cggtggtgcc gaagtcgccc ccgtcgcgcg gcttgagccg ggcgatgcgg
    16441 tcggccagca gggccttgag ttcattggcc cgctccagcg gctggtcggg cgcgccacgg
    16501 gcggcgagcc gttcgtcgat catcggcagc gcggtcagcg gactggcgac cagcttggac
    16561 aggtcgccgt agtggccgag cgcgcggcgg gtgagccggg cgaaggtgtc ggcgtcgacc
    16621 tcgtcgagcg gattgtccga ccgcagcggc aacgcggcct cggtgcgccg cagcgtggcc
    16681 cggtcggcgc gcagtcccgg cgagcg
//
LOCUS       DASRJA010000063         6760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4174677,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000063 DASRJA010000000
VERSION     DASRJA010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4174677"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VFC01_02865"
     CDS             complement(<1..1244)
                     /locus_tag="VFC01_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HZN78618.1"
                     /translation="MRRLMSRTTLAVALVVVMVLGVIVGVRATGQAARTMVVGYFDNS
                     TGVFAGDDVRILGVPVGKVEKIEPQPQRVKISFWLDPKYKVPANVNAVILSPQLVTGR
                     AIQLTPAYTSGPTLQDGAEIPQNRTAVPVEWDDVRDQLQRLNELLQPTKPGGVSTLGE
                     FINTAANNLRGQGATIRDTVIKLSQTLSALGDHSKDLFGTFKNLSTLVSALHDSTDLL
                     EQLNRNLASVTSLVADDPNKVGQMVKDLNAVVGDLQSFAADNREALGTTSDKLASVTT
                     ALVESLDDIKQDLHIAPTALQNFSNIYEPANGSLTGALAVNNFANPIGFLCGAVQAAS
                     RLGAEQAAKLCVQYLAPIVKNRQYNWPPLGFDLFVGAQARPNEVTYSEDWMRPDFVPQ
                     GPSPSAPASPVQAPPLAAEAPAP"
     gene            complement(1244..2599)
                     /locus_tag="VFC01_02870"
     CDS             complement(1244..2599)
                     /locus_tag="VFC01_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN78619.1"
                     /translation="MKSFSERNPVTIGLIGITAVVAIALAALNYQKLPFLNPGKTYSA
                     QFADAGGLFTGATVEVSGYPTGKVSAIELDGSRVLVTFKIDKDIHVGDRSEARIRAKS
                     LLGTKVLEVVPRGEDPLVSTIPIDRTQSPYQLPDALGDLATTIGGLNTVQLSESLATL
                     AQTFSDTPEEVKSAVQGVARFAQTLNERDANLRRLLENAAKATGVLAKRTDQVVSLVR
                     DTNALLAELRTQSAALDQIWYNVSAAAQQLKGFIEENRQTLRPALDKLNGVLAIIDNR
                     KQRLQLAIKGLGTYAMSLGESIGSGPFFKAYVVNLLPGQFVQPFVDAAFSDLGLDPAT
                     LPPSKLTDPQTGQPGTPPLPVPFPRTGQGGEPHLTLPDAITGNPGDQGCGPPGLPLPG
                     PTGCYPYREPLPAPPPGGPPPGPPAPSPPELQSVPEPTPAPVYVPAPGEAPPIPRGGQ
                     S"
     gene            complement(2596..3621)
                     /locus_tag="VFC01_02875"
     CDS             complement(2596..3621)
                     /locus_tag="VFC01_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HZN78620.1"
                     /translation="MENLRGAAWRLGIFLAICLLGTFVLVTVFAQFRFEKGKNYVAEF
                     TNVSGLRDGDIVRIAGVEVGKVQKISVNRDATVRVEFSADDSVVLTEGSRAIIRYDDL
                     IGNRFLALTEGAGGIKPLKPGDTIPVDRTEPALDLDALIGGFRPLFRALNPDQVNELT
                     GQLIEAFQGQGPTIGSFLNQAAAVTNTLADRDLLIGQVIDNLNVVLGSLGGQSERFDK
                     AVTSLSELVHGLAQRKTDLTNAVAYIDAGAGSFADLFTEVREPFQKVVHELDRASGIA
                     VADHEYLDRFLDQLPDKYRALNRQAMYGDFFSFYLCDAVLKLNGKGGQPVYVKVAGQS
                     TGRCAPK"
     gene            complement(3653..5032)
                     /locus_tag="VFC01_02880"
     CDS             complement(3653..5032)
                     /locus_tag="VFC01_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN78621.1"
                     /translation="MEPRPGERRIHPGWWALMLFAFTALFLFVTSAFFLGTFKSSVPV
                     TLTSDRSGIIMETNAKVKMRGVQVGRVSEVGTSKDGAKLTLEISHDQIRYIPANVEAK
                     IDVTTAFGAKFVDLVYPENPSKERLAAGAVLRSTNVSTEVNTVFENVVDLVKMIDPAK
                     LNAVLTAVADAVRGQGERMGQATTDLNQVLMALNDRSEIIRQDWRSFKNFNDTYAAAA
                     QDILTVLDAASTTSSTVVKHSASLDNLLLNVIGFGNAGTNLLVTSKDNLVASANVLEP
                     TTNLLLKYSPTYTCLLQGQTNNLKDGAYSVFGGADGRTLQFDVALLLGNDPYQYPDNL
                     PIVGAKGGPGGKPSCGSLPDVSKNFPVRQLVTNTGWGTGVDIRPNPGIGHPCYADWFP
                     ATRAVPEAPKIRQCIPGPAVGPVPYPGAPPYGAPLYGPGGVPLWPGVPPAGPEPGPAQ
                     PEENGQTPP"
     gene            complement(5037..5891)
                     /locus_tag="VFC01_02885"
     CDS             complement(5037..5891)
                     /locus_tag="VFC01_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN78622.1"
                     /translation="MTATVPSRLHPRLRSTAKSVVAEWNRIGSQAQFYVKTLTSIPDA
                     VINYRTELWRLIAQMGLGSGALAVVGGTVAIVGFLTITTGALVAVQGYNQLASVGFEA
                     LTGFASAFFNVRLIVPGTTSVALSATIGAGATAQIGAMRINEEIDALEVIGIRSITYL
                     ASTRVLAGVVVVIPLYCVAVMMAFLAARTGTTAIYGQGSGVYDHYFGTFLNPTDLIWS
                     FFQVVAIAVVIMLVHTYYGYNASGGPAGVGEAVGRAVRTSMVIAAVEIAFISLAVYGQ
                     SGNFNLAG"
     gene            complement(5901..6671)
                     /locus_tag="VFC01_02890"
     CDS             complement(5901..6671)
                     /locus_tag="VFC01_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN78623.1"
                     /translation="MVSTYLGGKPVRAMGGFFAMSLDTFVQMVKPPFAWKEYILQSWF
                     VARVSVLPALMLTMPYSVLLVFTFNVLLTEFGASDFSGTGAAIGTVNQIGPIVTVLVV
                     SGAGATAMCADLGARTIRDELDALRVMGINPIQALVVPRVLAATTVALALSATVILVG
                     LSGAFFFCVYVQHVSPGAFVAGLTLLTGMADVIVSLVKATLFGMAAGLIACYKGISVG
                     GGPAGVGNAVNETVVFTFMVLFAINIIVTAVGIQFTVQ"
BASE COUNT         1215 a         2143 c         2170 g         1232 t
ORIGIN      
        1 ggggccggcg cctcggcagc caggggtggg gcctggaccg gcgaagcagg cgcgctcgga
       61 cttggaccct gcggaacgaa gtccggacgc atccagtcct cgctgtacgt gacctcgttg
      121 ggtcgggcct gtgctccgac gaaaagatca aaccctaagg gcggccagtt gtactggcgg
      181 ttcttcacga tcggcgccag atactgcacg cagagtttcg cagcttgctc ggcgcccaat
      241 cgggaggcgg cctgtaccgc cccgcagagg aacccaatcg ggttggcgaa gttgttgacg
      301 gccagcgcgc cggtaagcga accgttggcc ggctcgtaga tgttgctgaa gttttgaagc
      361 gccgtcgggg cgatatgcag gtcctgcttg atgtcgtcga ggctttcgac cagcgccgtg
      421 gtgaccgacg ccagtttgtc cgacgtggtg ccgagcgcct cgcggttgtc cgcagcgaag
      481 ctctgcaggt cgccgacgac cgcgttgagg tccttgacca tctgaccgac cttgttcggg
      541 tcgtcggcca ccagcgatgt cacggatgcc aggttgcggt tcagctgctc gagcagatcc
      601 gtgctgtcat gcagcgccga caccagcgtc gacagatttt tgaaagtgcc gaagaggtct
      661 ttgctgtggt cgccgagtgc ggacaatgtc tgcgacagct tgatgaccgt gtcgcggatg
      721 gtggcaccct gcccgcgcaa gttgttggcg gccgtgttga tgaactcacc caacgtgctc
      781 acgccgcccg gcttggtggg ctgtagcagt tcgttcagcc tctgaagttg gtcgcggacg
      841 tcatcccatt cgactggcac cgcagtgcgg ttctgcggga tttccgcgcc gtcctggagc
      901 gtcggaccag aggtgtatgc cggcgtcagc tgaatggccc gacccgtcac cagctgtggt
      961 gacaagatca ccgcgttgac gttggcgggg accttgtact tgggatccaa ccagaacgag
     1021 atcttgaccc gctggggctg cggctcaatc ttttctacct tgcccaccgg tacgccgagg
     1081 atgcgtacgt cgtcgcccgc gaacaccccg gtgctgttgt cgaagtagcc gaccaccatc
     1141 gttcgggcag cctgaccggt ggcacgcacc ccgacgatca ccccgagaac catcaccacg
     1201 acgagggcga ccgccagggt ggtgcgggac attagccttc gcatcatgat tgacctcccc
     1261 gcggaatcgg cggtgcttca cccggtgcag gcacgtagac cggtgcgggt gtcggctccg
     1321 gtacggactg cagctcgggc ggcgacggtg cgggcggccc gggcggcggc ccgcctggcg
     1381 gcggggcagg cagcggttcg cggtaggggt agcagccagt agggccgggc agcggcagac
     1441 cgggcggacc gcacccctga tctccggggt tgccggtgat cgcgtcgggc agtgtgagat
     1501 gcggctcgcc gccctggccg gtgcgcggga acggcaccgg caacggcgga gtccccggct
     1561 ggccggtctg cgggtcggtc agcttggacg gcggcaacgt cgccgggtcc agtccgaggt
     1621 ctgaaaatgc ggcatcgacg aacggctgga cgaattgacc gggcagcagg ttcaccacgt
     1681 atgccttgaa gaagggtcct gagccgatcg attcaccaag tgacatggca taggtgccga
     1741 ggccttttat ggccagttgc aggcgctgct tgcgattgtc gatgatcgcc agcaccccgt
     1801 tgagcttgtc cagagccggc cgcaatgttt gtcggttttc ttcgatgaac cccttgagtt
     1861 gctgtgccgc cgcggaaacg ttgtaccaga tctgatccaa tgcggcgctt tgcgtccgca
     1921 gttccgcaag cagcgcgttg gtgtccctca ccaggctgac cacctggtct gtgcgcttgg
     1981 cgagcactcc cgtcgccttg gctgcgttct ccagcagcct gcgcaggttg gcatcgcgtt
     2041 cgttgagtgt ttgcgcgaac cgcgccacgc cttgtaccgc gcttttcacc tcttccgggg
     2101 tgtccgagaa ggtttgcgcc agggtggcga gcgattccga tagctggacg gtgttcagcc
     2161 cgccgatcgt ggtggccaag tcgccgaggg cgtccggcag ctggtatggc gactgcgtcc
     2221 ggtcgatcgg gatggtgctg acgagtgggt cctccccgcg ggggacgacc tccaggacct
     2281 tggtacccag aaggcttttg gctctgatcc tggcctcgga gcggtccccc acatgaatgt
     2341 ccttgtcaat cttgaacgta acgagaacac gggatccgtc gagttcgatg gcggacacct
     2401 tccccgtggg ataaccggag acctcgacgg tggcgcctgt gaatagcccg cccgcgtcgg
     2461 cgaactgggc cgagtaggtt ttgcccgggt tcaaaaacgg cagcttctgg tagttcaacg
     2521 cggccagcgc aatcgcgacc actgccgtga tgccgatgag gccgatggtc acggggttgc
     2581 gctcggagaa cgatttcatt tcggcgcgca ccgccccgtg ctctgaccag cgacctttac
     2641 gtacaccggc tggccaccct tcccattgag cttgagcacc gcatcgcaga ggtagaagct
     2701 gaagaagtcg ccgtacatcg cctgacggtt gagcgcccgg tacttgtctg gcagctggtc
     2761 gaggaaacgg tcgaggtact cgtgatcggc caccgcgatg cccgaggcgc ggtcgagttc
     2821 gtgaaccacc ttttggaacg gttcgcgaac ctcagtaaac aaatcggcga acgagccggc
     2881 gccggcatcg atgtaggcca ccgcgttggt gaggtcggtc ttgcgttgcg ccaacccgtg
     2941 gacgagttcc gacagcgacg tcaccgcctt gtcgaacctc tcgctctggc cgcccagcga
     3001 ccccaacacc acgttgaggt tgtcgatgac ctgtccgatc agaaggtctc ggtcggccag
     3061 cgtgttggtt accgcagccg cctggttcag aaacgacccg atcgtgggac cctgtccctg
     3121 aaacgcctct atcagctgtc ccgtcaactc gttgacctgg tccgggttca gcgcacgaaa
     3181 cagcggccga aagccgccga tcagcgcgtc cagatccaat gcgggctcgg tgcggtcaac
     3241 aggaatcgtg tcaccgggct tgaggggctt gatccccccg gcaccttctg tgagcgccag
     3301 gaagcggtta ccgatcaggt cgtcgtacct gatgatcgcc cgggatccct cggtgaggac
     3361 caccgagtcg tcggccgaga actcgacgcg caccgtcgcg tcccggttga cggagatctt
     3421 ctgaaccttg ccgacctcca ccccggcgat ccggacgatg tcgccatccc tgaggccgga
     3481 aacgttggtg aactcggcga cgtagttctt gcctttctcg aaccgaaact gcgcgaagac
     3541 cgtcaccagt acgaatgtgc caagcaggca aatcgccagg aagatgccaa ggcgccacgc
     3601 cgcgcctctc agattttcca cgtcttctcc tgcgtcggta tgtcgtcggc gatcacggtg
     3661 gcgtctggcc gttctcttcg ggttgcgccg gacccggctc gggccccgcc ggcggcacgc
     3721 ccggccacag cggaaccccg cccggcccat acaggggcgc gccgtacggc ggcgctccgg
     3781 gatacgggac cgggccgact gccggccccg ggatgcactg gcggatcttg ggcgcctccg
     3841 gtacggcgcg ggtcgccggg aaccagtcgg catagcacgg atgcccgatg ccagggttgg
     3901 gccgaatatc cacgccggtc ccccacccgg tgttcgtgac gagctggcgc accgggaagt
     3961 tcttcgaaac atccggcagc gacccacagc tcggcttccc gcccggtccc cccttggccc
     4021 cgacaatcgg cagattgtcg ggatactggt aggggtcgtt gccgagcaac agggcgacgt
     4081 caaactgaag cgtccggcca tcggcgccgc cgaacaccga ataggccccg tccttcaggt
     4141 tgttcgtctg gccctgcagc aagcaggtgt atgtggggct gtacttgagc agcaggttcg
     4201 tggtgggctc gagaacgttg gccgatgcga cgagattgtc cttgctggtc acgagcaggt
     4261 tggtcccggc gttcccgaag ccaatgacgt tgagcaggag gttgtccagg gacgccgagt
     4321 gcttcacgac cgtgctactg gtagtgctgg cggcgtccaa gaccgtcagg atgtcctggg
     4381 cggccgcggc gtaggtgtcg ttgaagttct tgaaggagcg ccaatcctga cggatgatct
     4441 cgcttcggtc gttgagcgcc atcaacacct ggttgaggtc ggtggtggcc tggcccatcc
     4501 gttcgccctg tccgcgcacc gcgtcggcca ccgccgtcag caccgcgttc agcttcgccg
     4561 gatcgatcat cttgaccagg tcgacaacgt tttcgaagac cgtgttgacc tctgtgctga
     4621 cattcgtcga acgcagcact gcgccggcag ccagccgttc tttactcggg ttctccggat
     4681 acaccaggtc gacgaacttg gcgccgaacg ccgtggtgac gtcgatcttc gcctcgacgt
     4741 tggccgggat gtaccggatc tggtcgtgac tgatctccag cgtcagcttg gcgccgtcct
     4801 tgctggtgcc aacctcgctg acccgcccga cctggacgcc acgcatcttg accttcgcgt
     4861 tggtctccat gatgatcccg gaccgatccg aagtcagcgt caccggcaca gaggacttga
     4921 acgtgccaag gaaaaacgca gaggtcacga acaggaacag ggcggtaaac gcgaacagca
     4981 tcaaggccca ccacccgggg tggatgcgac gttctcctgg ccggggttcc atgccgctat
     5041 ccagccaggt tgaagttgcc ggactggcca taaacggcca gcgagatgaa cgcgatttcg
     5101 accgccgcga tcaccatcga cgtacgcacc gcccgaccca cggcctcacc aacaccggcg
     5161 gggccaccgg acgcgttgta cccgtagtag gtgtgcacca acatgatcac caccgcgatc
     5221 gcgaccactt gaaagaacga ccagatcaga tcggtgggat tgaggaaggt gccgaagtag
     5281 tggtcataca caccggagcc ttggccgtag atggcggtgg tgccggtgcg cgcggccaga
     5341 aaggccatca tgacggccac gcagtacagc gggatcacta cgaccactcc ggcgagcacc
     5401 cgggtggatg cgaggtaggt gatgctgcgg ataccgatca cctcgagcgc gtcgatctcc
     5461 tcgttgatcc gcatcgcgcc gatctgggcg gtggcgccgg caccgatggt cgccgacaac
     5521 gcgacggacg tcgttcccgg cacgatgagc cggacattga aaaacgccga cgcgaacccg
     5581 gtgagggcct cgaaacccac ggacgccaac tggttgtaac cctgcaccgc caccaacgcg
     5641 ccggtggtga tcgtgaggaa gccgacgatc gccacggttc caccgacgac cgcgagcgcc
     5701 ccagaaccca atcccatctg tgcgatcagc cgccacagct cagtccggta gttgatgaca
     5761 gcgtcgggaa tcgacgtcag tgtcttgacg tagaactgtg cttgcgaccc gatccggttc
     5821 cactcggcga caacgctttt cgccgtgctc cgcagccgcg gatgtagcct gctcggaacg
     5881 gtagccgtca tgggttcacc tcattgcacc gtgaactgga taccgacggc cgtcacgatg
     5941 atgttgatcg cgaacagcac catgaaggta aacacgacgg tttcgttgac cgcgttgccg
     6001 acgccggccg gaccaccgcc gaccgagatc cccttgtagc aggcgatcag gcccgcggcc
     6061 atcccgaata acgtcgcctt gaccagcgag acgatgacgt cggccatgcc ggtgagcagc
     6121 gtcagacccg ccacgaacgc gcccggcgag acgtgctgca catacacgca gaaaaagaac
     6181 gcgccggaca ggcccaccag gatcacggtg gccgacagcg ccaaggccac ggttgtagct
     6241 gccaacacgc ggggaaccac caacgcctgg atggggttga tgcccatcac tcgcagcgcg
     6301 tcgagttcgt cacgaatggt ccgcgccccc agatcggcgc acatggcggt ggcgccggcg
     6361 ccggagacaa ccagcaccgt gacgatcggg ccgatctggt tgactgtgcc aatcgctgcg
     6421 cctgttccgg agaagtccga tgcaccgaat tcggtcagca ggacattgaa cgtgaacaca
     6481 agcagcaccg aatacggcat cgtcagcatt agcgccggca agaccgatac ccgtgccacg
     6541 aaccagcttt gaagtatgta ttccttccag gcgaacgggg gcttgaccat ctgcacgaag
     6601 gtgtccagtg acatcgcgaa gaagccgccc atcgcgcgga ccggcttgcc gcccagatac
     6661 gtcgacacca tcagctacgc ccccacgacg atcgaatggt gtccttgatg atgcgacccg
     6721 tgacggtgat acgtacttgt aattgaagaa ttgttatttt
//
LOCUS       DASRJA010000064         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1913132,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000064 DASRJA010000000
VERSION     DASRJA010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913132"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(751..1596)
                     /locus_tag="VFC01_02895"
     CDS             complement(751..1596)
                     /locus_tag="VFC01_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN78624.1"
                     /translation="MKYDLTNRTVAITGAAGGLGQDLAKALRARGASVALLDLDADAV
                     GDLAEHLGGDSVARGWKADVRNLDNLTEVMDSVAAHFEGLDVVVAGAGTGGPIGPIAG
                     MAPGDWERTIDVNLNGVWRTFKAALPHVAFQRGHLLAVASMASFVHSPLHSPYSASKA
                     GVLGMCNSLRPELRQFGVTVGSAHPSFFRSFMVDEVLDDPVARNVWNDFSGLFKLVPR
                     ETVVDAIVGGIERRAEQIVVPRRFTASAKLSALFRPAVERFVFPDSRVMQAIARFTTT
                     PFGTT"
     gene            complement(1596..>2310)
                     /locus_tag="VFC01_02900"
     CDS             complement(1596..>2310)
                     /locus_tag="VFC01_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN78625.1"
                     /translation="GQPRQLLNIKRQLADWAAAIRFARARDDVDGSRIILWGSSFSGG
                     HVITTAARDAGISAAIAQCPFTDGVTSVLAMPRLTALKVGARALIDLTASQFGKGPVL
                     LPVVGPPGSTAVMTAPDALPAMLAMVPNGVPWRNEVAARVALEVLTYRPGRTAARVQC
                     PVLFGICEADTVTPADTTLRYANTAPRAEVRLYPEGHFDIYLGDAFERTVSDQIDFLH
                     RHVPVNPTSPAPTQQQETA"
BASE COUNT          442 a          700 c          758 g          410 t
ORIGIN      
        1 cgtcgcagag acgcttcaac gtaaccagca gcgccgcagc cgaacgatct ggaaggaaca
       61 cctcaaaact gtcgatgcct ggcgcgccgg atatgaacgc atggctgccg cggccacgcc
      121 ttctgccgaa atcttcctcg acacaggtgg actggaggtc tgacggctgg cgaactgcga
      181 ctcaaaatcg acgaccgtca atgttgaaat gagcgccgac gagctgacgc agatgtcatt
      241 gcggagcgcg gggaagaggg ccaggagtgt cactccgtct atgcacaacg agcatgtggt
      301 aaggcgacta tgcaggcgta cgcccccccg agacaacatc gcagctgatg tttgtgaacc
      361 ccaacgatgt gcggctcgag cccactcggc gtttgcagca agaggatccg ggcaccaaaa
      421 cccgtgaggc cgacgctaat ggttcccgat tcgcattagc acttctaatg ccttagggcg
      481 acatagttgg gcattatcgg actgcgtcgt cgttacccgg gcgcgcagga gcaacgtcgg
      541 attgggcgga ctgcaaccgc ttggtgacgg gcccgtgcgg attacgtttc gccgtgccag
      601 cgacacaacg agatgattgt tcgatgccgc ttgataggtc agtggtacat cagacggagt
      661 cgcccactaa caagacgcag caccgagcat gtgtcgccat gcgcgatgac atcagaacgt
      721 catagacgcg gccgcgtact tgagagatgc ttacgtcgtc ccgaacgggg ttgtggtgaa
      781 tcgtgcaatc gcctgcatca cgcgagagtc cgggaatacg aatcgctcca ccgccgggcg
      841 aaataatgcc gagagcttgg cggaggcggt gaaccgccgt gggacgacga tttgttccgc
      901 gcgccgctcg atacctccga caatggcgtc caccaccgtc tcgcgcggca ccagcttgaa
      961 cagcccgctg aagtcgttcc atacgttgcg cgctacgggg tcgtcgagaa cctcgtcgac
     1021 catgaacgat cggaagaaac tgggatgtgc acttcctacg gtcacgccga actggcgcag
     1081 ttcgggccgc aacgagttgc acatcccgag cacgccggcc ttgctggcgc tgtaaggaga
     1141 gtgcagcggc gagtgtacga agctggccat cgacgctacg gccaggaggt ggccccgctg
     1201 gaaggcgacg tggggaaggg cggctttgaa tgtgcgccat acgccgttga ggttgacgtc
     1261 gatcgtgcgt tcccagtcgc cgggcgccat gcccgcaatc ggtccgatgg gaccgccggt
     1321 acctgcgccg gcaacgacga cgtccagccc ttcgaaatgc gctgcgaccg aatccattac
     1381 ctcggtgagg ttgtcgaggt tgcggacgtc ggctttccag ccacgggcca cgctgtcacc
     1441 gccgagatgc tcggcgaggt cgcccactgc gtcggcatcg aggtcgagca acgcgacgct
     1501 cgcccctcga gcgcgtagcg ctttggccag atcctggccg aggccgccgg ccgcgccggt
     1561 gatcgcgact gtgcggttcg tgaggtcgta cttcattacg cggtctcctg ttgttgggta
     1621 ggcgctggcg aggtcgggtt gactggcacg tgccggtgta ggaagtcgat ctggtcgctc
     1681 acagtgcgtt cgaaagcatc gcccaggtag atatcgaagt gtccttccgg gtaaagcctc
     1741 acttctgcgc ggggcgcggt gttggcgtag cgcagcgtcg tatcggcggg tgtcacagtg
     1801 tccgcctcgc agataccgaa cagaaccggg cactgtacgc gggcggcggt ccggcctggg
     1861 cggtaggtaa gcacctcgag cgccacgcgc gccgccacct cgttgcgcca cggcacgccg
     1921 ttggggacca tcgccagcat ggcgggcaac gcatcgggag ccgtcatgac tgcggtggaa
     1981 ccgggcggcc cgaccacggg cagcagcaca ggacctttgc caaattggct cgctgtcagg
     2041 tcgattagtg cacgagcgcc gaccttcagt gcggttagtc gaggcatcgc caacactgag
     2101 gtcaccccgt cggtaaacgg gcactgcgcg atcgcagcac tgattccggc atcgcgagcc
     2161 gcagtggtaa tgacgtggcc gccgctgaat gagcttcccc acagaattat tcgggagccg
     2221 tcgacatcgt cgcgggcgcg cgcgaatctg atcgcggccg cccagtctgc caattggcgc
     2281 ttgatgttga gaagctgccg aggctgaccg
//
LOCUS       DASRJA010000065         3325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1074209,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000065 DASRJA010000000
VERSION     DASRJA010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074209"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..>3325
                     /locus_tag="VFC01_02905"
     CDS             191..>3325
                     /locus_tag="VFC01_02905"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN78626.1"
                     /translation="MLAHSVPLSGLSANTTYNYRVRSRDPAGNERVSANSTFKTAIAD
                     TVPPTVQSLNRVGASPTNAGSLSWTVTFSEPVTGVDTGDFALVGSVTGAAISSVTGSG
                     NQYVVAVGTGSGNGTLGLNLVDNDSIVDTAANPLGGAGLGNGSFTGQSYTIDKTAPSV
                     TIDQAAGQADPTNTSPISFTAVFSEAVSGFAGGDVSFVGSTMGGTLVATVSGTGPSYS
                     VAVTGMTGTGIVVASIPTGAASDAAGNASVGSTSTDNTVTATAPVDATPPTVSVTAPA
                     AGAIVTGTVTVTADAADDVGVTGVQFLLDGANLGAEDLTAPYSVSWNSTAVADGSHTL
                     LARARDAAGHMTTSSPVAVTVANAQSAGLVAAWSFDETSGSLATDSSANGNTATLLNG
                     VARTPGTSGGGLTLDGVDDYLNVPNSPSLDISGTGLTLRMSIKPQATGADTVVLSKAW
                     GATMTSPFYQYGLELAGGTVPSLHIGTSGGVLAASMGSALVTNQWSDLAISFNGAQVQ
                     FYVNGAPVKSVLLLASISARGRSLRLGADATPAQFFKGSLDEVRIYNRALTTAELASG
                     NHFQNEILTVGLNLPTTMAFLPGGAMLVGELGGTVRRALPPYTQLAPTPFLQITNIGQ
                     NFANQGLYTIAVDPAFTTNHYIYVCYTLGTPNHDRLSRFTVNQALTSAGSELVLYEDL
                     GISGADHHGGAIMFGNDNMLYLTTGDQFDGPTAQDMGNPEGKILRLNPVDGSAAPGNP
                     FLNTPGVDPRIWASGLRNPFRGYYDAPTGRMLIGDVGYQTIEEINIGAARANYGWPNA
                     EGPSNNPAYTNPVFSYPHAGRDSAVVGGFVYHGTQFPSSYQGSYFYADYAQHWIKRLT
                     FGANGNVTGAFNFEPTDGSPDGPFGDIVWLTEGPEGALYYVDLGFQLGGNGAGAVRRI
                     SYVSTNQAPLAAASAAPTTGPKPLTVTFSSAGSSDPEGTPLTYSWDFGDGVVSTDANP
                     THTYTTAGLYQARLTVSDGVNSSLAAPVSISVGSPPAITTFNTTPADGGFFVAGDVVS
                     FSATATDPDGVLSPS"
BASE COUNT          604 a         1160 c         1046 g          515 t
ORIGIN      
        1 gtcctcactg ccgatcaccg acaccatcgt gctcgacacg acggcaccga ccatctcatc
       61 tcgcaccgaa acatcgatca cgagcaatgc cagcacgatc acctggacca caaacgagcc
      121 cgccacgtcg caggtcgact acgggctcac cactggctac gggtcaacca cgccgctcga
      181 tcctgcgctg gtgctcgctc acagcgtccc gctcagcgga ctgtcggcaa acacgacgta
      241 caactaccgc gttcgctcgc gcgatccggc tggcaacgaa cgcgtcagcg ccaacagtac
      301 gttcaagact gccatcgccg acaccgtgcc gccgaccgta cagtcgctga accgggtagg
      361 ggcatcgccg acgaacgccg gaagcttgtc ctggacggtg acgttcagcg aacccgtgac
      421 cggagtggac acgggagact tcgcgctcgt gggctcggtc acgggcgcgg cgatctcgtc
      481 ggtcaccggc agcggcaatc agtatgtcgt cgcggtgggc accggctcgg gcaatggcac
      541 gttgggcctc aatctggtgg acaacgattc gatcgtcgac accgccgcga atcccttggg
      601 cggcgccggc ttgggcaacg gcagcttcac cggccagagt tacaccatcg acaagacggc
      661 gccgagtgtg acgatcgatc aggcggccgg tcaggcggat ccgacgaata cgagtcccat
      721 cagcttcacg gcggtcttca gtgaggcggt gtcgggcttc gccggcggag acgtcagctt
      781 cgtgggcagc acgatgggtg gcacgctcgt ggcgaccgtg tctgggaccg gtcccagcta
      841 cagcgtcgcg gtgacaggga tgacggggac ggggatcgtc gtggcgagca tccccactgg
      901 agcggcgagc gatgcggcgg gcaacgccag cgtggggtcg acgagcacgg acaacacggt
      961 gaccgccacg gcgccggtcg atgcgacccc tccgacggtg tcggtcaccg caccggccgc
     1021 gggagcgatt gtcaccggca cggtcacggt cacggcggac gcagccgatg acgtcggcgt
     1081 gaccggcgtc cagtttctgc tcgacggcgc gaacctgggc gccgaggacc tcacggctcc
     1141 gtacagcgtg agctggaaca gcaccgcggt ggccgacgga tcgcacacgc tgcttgcccg
     1201 ggcgcgcgac gcggctggcc acatgacgac ctcgtcgccg gtcgccgtca cggtcgccaa
     1261 cgcccagtcc gcagggctcg tcgcagcctg gagcttcgac gagacttccg gatcgctcgc
     1321 caccgacagc tccgccaacg gcaataccgc gaccctgctc aatggggtcg cgcgtacgcc
     1381 cggaacgtcc ggtggcggcc tgaccttgga cggcgtcgac gattatctga atgttcccaa
     1441 ctcgccatcg ctcgatatct caggcaccgg cttgacgttg cggatgtcga tcaagccgca
     1501 ggccaccggc gcggacacag tcgtcctgag caaggcatgg ggggcgacga tgacgagtcc
     1561 gttctaccag tacgggctgg aattggccgg cggtacggtg ccaagcctcc acatcggcac
     1621 gtcgggtggg gtgctggccg catccatggg aagcgcattg gtcaccaacc agtggagcga
     1681 cctcgcgatc agcttcaacg gtgcccaggt gcagttctat gtcaacggcg ctccggtgaa
     1741 gagcgtattg ctgctggctt cgatctcggc acgaggcagg tcgttgcggc tcggcgccga
     1801 cgccacgccg gcacagttct tcaagggttc gctcgatgag gtgcggatct acaaccgggc
     1861 gctgacaaca gcagagctcg cgtccggcaa ccacttccag aacgagatcc tgacggtcgg
     1921 actcaacctc ccgaccacga tggccttcct tccgggcggt gccatgctgg tcggcgagct
     1981 cggtggaaca gtccgacggg cattgccgcc atacacccag ctcgccccga caccgttcct
     2041 gcagatcacc aacatcggac agaacttcgc gaaccaaggg ctctacacga tcgccgtcga
     2101 tcccgccttc accaccaacc actacatcta cgtctgctac acgctcggca cgcccaatca
     2161 cgatcgcctg tcccgattca ccgtcaacca ggccctcacg agcgccggca gcgaactcgt
     2221 gctgtacgag gacctgggca tcagcggcgc tgaccaccac ggtggcgcga tcatgttcgg
     2281 caacgacaac atgctctacc tcacgaccgg agaccagttc gacggtccga cggcgcagga
     2341 catgggcaac ccggagggca agatcctgcg cttgaatccc gtcgacggct ccgccgctcc
     2401 tggcaatccc ttcctcaaca cgccgggtgt cgaccctcgc atctgggcct ccggactgcg
     2461 caaccctttc cgcgggtact acgacgcgcc gacggggcgg atgttgatcg gcgacgtggg
     2521 ctatcagacc atcgaggaga tcaacatcgg cgccgctcgg gcaaactacg gctggcccaa
     2581 cgcggaaggt ccgagcaaca atccggccta caccaacccg gtcttctcct atccccacgc
     2641 gggccgcgac tcggccgtgg tgggcgggtt cgtctaccac gggacgcagt ttccgagcag
     2701 ctaccagggc agctacttct acgccgacta cgcgcagcac tggatcaagc ggctgacctt
     2761 cggcgccaac ggcaacgtca ccggcgcttt caacttcgag cccaccgacg gatcgcccga
     2821 cggacccttc ggtgacatcg tgtggctgac cgagggaccg gagggcgcgt tgtactacgt
     2881 cgacctcggc ttccagctcg gcggcaacgg tgcgggggcg gtccggcgga tcagctacgt
     2941 gagcaccaac caggcaccgc tcgcggccgc gagcgcggca ccaacgactg ggcctaagcc
     3001 actcaccgtg acgttctcga gcgctggctc ctccgacccg gagggaacgc cgctcacgta
     3061 ctcgtgggac ttcggcgacg gcgtggtgtc gaccgatgcc aacccgaccc acacctacac
     3121 cacagccggt ctgtatcagg cgcggctcac ggtgtccgac ggcgtcaaca gctcgctcgc
     3181 cgcaccggtg agcatcagcg tcggcagccc gccagcgatc acgacgttca atacgacccc
     3241 cgccgatggc ggtttcttcg tggccggaga cgtggtgtcc ttcagcgcca cggcgaccga
     3301 ccccgacggc gttctgtccc cgagc
//
LOCUS       DASRJA010000066         3681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1347974,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000066 DASRJA010000000
VERSION     DASRJA010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347974"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFC01_02910"
     CDS             complement(<1..239)
                     /locus_tag="VFC01_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HZN78627.1"
                     /translation="MRHYYTADEIREAEAPLLASLPEGGLMRRAAFGLATAVVRELIL
                     LTGGISGRRVCAVVGSGDNGGDALWAATFLRRRGAS"
     gene            complement(281..754)
                     /locus_tag="VFC01_02915"
     CDS             complement(281..754)
                     /locus_tag="VFC01_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HZN78628.1"
                     /translation="MSATVDWQVVLLDLLNTTPMVDGQMIDQLDARWLRGRGAAKDDL
                     DAVRQLRDDLQRVVRMQADASVLRRYLSDVRQVPGIGSDGVAWSLEAGWPARVVLAWG
                     ELERDMPGRLRPCANDECRLFLLDHSRAGTARWCSMSGCGNRMKARRHYSRTRRD"
     gene            839..1708
                     /locus_tag="VFC01_02920"
     CDS             839..1708
                     /locus_tag="VFC01_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN78629.1"
                     /translation="MVTHHRFSEIAGRRIFYREAGSPAAPAVVLLHGTPASSHQYRNL
                     IPALADRYHVIAPDYPGFGHSDVPSVDEFKYTFDSLADHVEELLDHLGLQRYAIYVQD
                     YGAPVGWRLALRHPERIAAIITQNGNAYVEGFVGDAMEPLFAYGRERSDANASALREI
                     IGMDGLKWQFTHGVSDPSVVDPDTWINAHSAVASSPERVDAQQELFADYSSNVDLYPR
                     VHEYFRTSQVPLLAVWGRNDQIFGPDGALAFARDLPQAEINLLDGGHFLLESRLSEVV
                     ALIRPFLERHLLG"
     gene            complement(1697..2074)
                     /locus_tag="VFC01_02925"
     CDS             complement(1697..2074)
                     /locus_tag="VFC01_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HZN78630.1"
                     /translation="MSLVEIIEGIGTAIDAIGVAVIAAGAIVALFMTFAKGRREEGGA
                     YEFFRRRLGRAILLGLEFLVAADIIRTVAVTPSGESVAVLGGIVLIRTFLSFSLQLEV
                     TGAWPWQQRSAAAAAKTEGRSAE"
     gene            complement(2102..3001)
                     /locus_tag="VFC01_02930"
     CDS             complement(2102..3001)
                     /locus_tag="VFC01_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN78631.1"
                     /translation="MPRRNVGHFKGERARAHYLSAYQTAMAGLPPVDESVDVPTSFGT
                     VRAYRFDGPSSGPPVVLLPGRNASTPMWRSNIPALLEHRTVICVDLLGEAGMSVQEKP
                     ITGPADEAQWVDDVLAGLGLHRTHLMGVSIGGWTAVNFAVHRPGRAASLSLLDPVFTF
                     APVPVKSMVIAAGMFLPLPDSVQRRVFSWISGGADVDDSLPEAALISAGSTDYVLRKA
                     MPKLFTNDQLRSLAVPVLALIAGRSVMLDAALAAARARNLLPRGQVELWPDASHAING
                     EYPGEIAQTAAEFWDEVDGVSSQ"
     gene            complement(3001..>3681)
                     /locus_tag="VFC01_02935"
     CDS             complement(3001..>3681)
                     /locus_tag="VFC01_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZN78632.1"
                     /translation="VLYTRAGPEIGVASTKTFLAQIAANYLVGLALAQARGTKYPDEV
                     EREYRDLEAMPDLVARVLDTVKPVSALAQRFAQSQTVLFLGRHVGYPVALEGALKLKE
                     LAYMHAEGFAAGELKHGPIALIEDDLPVIVVMPSPKNAATLHAKLLSNIREIQARGAI
                     TIVIAEEGDDTVRPYADHLIEIPAVSTLFQPLLSTIPLQVFAAGVAQARGYDVDKPRN
                     LAKSVTVE"
BASE COUNT          640 a         1225 c         1211 g          605 t
ORIGIN      
        1 gaggcacccc gtcggcgcag gaacgtcgcc gcccacagcg catcgccgcc gttgtcgccg
       61 gagccgacga cagcacagac ccggcggccg gagatgccgc cggtcagcag gatcagttcg
      121 cggacgacgg cggtggccaa cccgaacgcg gcgcgtcgca taagcccacc ctcgggcagc
      181 gacgccagca gcggcgcttc ggcttcgcgg atctcatcgg cggtgtagta gtgccgcatc
      241 ccagccagga ttccatgcct gcgacattga cagccaggtt tcagtcccgg cgggtccggc
      301 tgtagtggcg gcgcgccttc atgcggttgc cgcagccgct catcgagcac caccgggccg
      361 taccggcgcg agagtgatcg aggaggaata accggcactc gtcgttcgcg cacggccgca
      421 gccggcccgg catgtccctt tccagctcgc cccaggccag cacgacgcgg gccggccaac
      481 cggcctcgag cgaccaggcg acaccgtcgg acccgatgcc gggcacctgg cgcacatcgc
      541 tcaagtagcg gcgcaggacg gatgcgtcgg cttgcattcg gaccacgcgt tgcaggtcgt
      601 cgcgtagttg ccgcacggcg tcgaggtcgt ccttggcggc gccgcggccg cgcagccaac
      661 gcgcatccag ctgatcgatc atctgcccgt caaccatcgg cgtggtgttg agcagatcca
      721 gcaggacgac ctgccaatcc actgtcgcag acatgctaac catcctagag cacttgacag
      781 gttagatcca ggggtgttga ctgcacctaa ccatcacatc taatgataga ggttagaaat
      841 ggtcacgcat catcgcttct ccgagatcgc cggacgccgg atcttctacc gcgaggcagg
      901 ctcccccgcc gcgcccgcgg tggtgctgct gcacggcacg ccggccagct cgcatcagta
      961 ccgcaatctg atcccggcgc tggccgaccg gtaccacgtc atcgcgcccg actaccccgg
     1021 cttcggccac tcagatgtgc cgagtgtcga tgagttcaag tacaccttcg acagcctcgc
     1081 cgaccatgtc gaagagctgc tggaccactt gggtctgcag cggtacgcga tctacgtcca
     1141 ggactacggc gccccggtgg gctggcggct ggcgttgcgt caccccgagc gcatcgcggc
     1201 gatcatcacc cagaacggga atgcctatgt ggaggggttt gtcggcgacg cgatggagcc
     1261 gctgttcgcg tacggccgcg agcgttccga cgcaaacgcc tcggcgttgc gcgagatcat
     1321 cggcatggac ggcctgaaat ggcaattcac ccatggtgtt tcggacccgt cggtggtcga
     1381 ccccgacacc tggatcaacg cgcattcggc ggtggcgagt agcccggagc gggtcgacgc
     1441 acagcaggaa ctgttcgccg attacagctc gaacgtcgac ctgtacccgc gcgtgcacga
     1501 atacttccgt acctcacagg tgccgctgct ggcggtgtgg ggccgcaacg accagatctt
     1561 cggcccggac ggcgctttgg cgttcgcgcg ggacctgcct caggccgaga tcaacctgct
     1621 cgacggcggt cacttcttgt tggagagccg cctctctgag gtcgtcgcgc tgatccggcc
     1681 attcctcgag cggcacttac tcggctgacc ggccctcggt tttggcggcg gccgccgcgg
     1741 agcgctgctg ccatggccat gcgcccgtca cctccagttg gagggagaag ctgaggaagg
     1801 ttcggatgag cacgatgccg ccaagcaccg cgacgctctc gccgctcggt gtgacggcga
     1861 cggtgcggat gatgtctgcg gcgacgagga attcgaggcc cagcaggatc gcgcggccga
     1921 ggcgccgacg gaagaactcg taggcgccgc cctcctcacg acgacccttt gcaaacgtca
     1981 tgaagagggc gactatcgca ccggctgcga tgacggcgac accgatggcg tcgatcgccg
     2041 tgccgattcc ctcgatgatc tcgacgagag acacgacgtc atggtgtcac cgacgccgcc
     2101 gctattgcga cgacacgccg tccacttcgt cccagaactc cgccgccgtt tgcgcgatct
     2161 cgcccgggta ctcgccgttg atcgcgtgcg acgcgtcggg ccagagctct acttgtccgc
     2221 gcggcaagag gtttcgcgct cgtgccgctg cgagggcggc gtcgagcatc acacttcggc
     2281 ccgcaatgag cgccaacacc ggaacagcga gtgaccgcaa ctggtcgttg gtgaacaact
     2341 ttggcatcgc cttgcgtaac acgtaatcag tagagccggc ggagatcagc gcggcctcgg
     2401 gcagcgagtc gtcgacgtcg gctccgccgg aaatccagct gaacaccctg cgctgcacgg
     2461 agtccggcaa cgggagaaac attccggccg ctatcaccat cgatttgacc ggcaccggcg
     2521 cgaaggtgaa aaccgggtcg agaagcgaca gtgacgcggc acggccgggt cggtggaccg
     2581 cgaagttgac cgccgtccag ccgccaatcg agacacccat aaggtgcgtc cggtgcagcc
     2641 cgaggccggc cagcacgtcg tccacccact gtgcctcgtc ggcgggaccg gtgatcggct
     2701 tttcctgcac ggacatcccg gcctcgccga gcaggtcgac gcagatgacg gtgcgatgct
     2761 cgagcaacgc gggaatgttg ctccgccaca tcggcgtgga cgcgttgcga cccggcagca
     2821 gcaccaccgg tggaccggag gatggcccgt cgaaccggta ggcacggacc gtcccgaacg
     2881 acgttggcac atcgactgac tcgtcgacag gcggcaaccc agccatggcg gtctggtagg
     2941 cgctcaggta gtgcgcgcgg gctcgttcac ccttgaagtg tccgacgttt cggcgcggca
     3001 ctattcgacg gtgacggact tcgccaggtt gcgtggcttg tccacgtcgt agccgcgggc
     3061 ctgcgcgacg cctgcggcga acacctgcag cgggatcgtc gacaggagtg gctggaaaag
     3121 cgttgatacc gcaggtattt cgatcaggtg atcggcgtac ggccggacgg tgtcgtcgcc
     3181 ttcctctgcg atgacgatgg tgatcgcgcc acgcgcctgg atctcgcgga tgttcgacag
     3241 cagcttggcg tgcagcgtcg cggcattctt cggcgacggc atcacgacga tcaccggcag
     3301 atcgtcctcg atgagcgcga tcgggccgtg cttgagctcg ccggcggcga acccctcggc
     3361 gtgcatatac gccaattcct tgagtttgag cgcaccttcg agcgcgacgg gatagccgac
     3421 gtggcggccg aggaacaaca cggtctgtga ctgcgcaaac cgttgggcca gtgcggagac
     3481 gggtttgacg gtatccagca cgcgggccac caggtcgggc attgcctcca gatcgcggta
     3541 ctcccgctcc acctcgtcgg ggtacttggt accgcgggcc tgcgcaaggg caagcccgac
     3601 aagataattc gccgcaatct gcgcaaggaa cgtcttggtg gatgcgacgc cgatctccgg
     3661 cccggcgcgg gtgtagagca c
//
LOCUS       DASRJA010000067        19268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3326559,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000067 DASRJA010000000
VERSION     DASRJA010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 19268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19268
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3326559"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFC01_02940"
     CDS             complement(<1..234)
                     /locus_tag="VFC01_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZN78633.1"
                     /translation="MLNRTARIQLVVFAVVTVLTVGAISLFYLRVPARLGIGAYQVTA
                     NFVAGGGLYENANVTFRGVQAGRVESVKLTDDGV"
     gene            complement(234..1433)
                     /locus_tag="VFC01_02945"
     CDS             complement(234..1433)
                     /locus_tag="VFC01_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN78634.1"
                     /translation="MTRKSLGINVGRSTSRTVAIGSGVVLLAGCQFGGLNSLNMPGTA
                     GHGPGAYSITVELPDVATLPQNSPVLVDDVTVGSVSGVEAVQRPDGTFYAAVKVSLEP
                     NVKLPANATAKVGQTSLLGSQHIQLSAPVGQPAVGELRDGSEIPQSQGGRYPTTEEVL
                     SALGVVVNKGNLGALQDITQEVSNAVAGRQGQFADLIPRLAELAASLNRQTDDIIAAA
                     EGLNRFAGVLARQRDSLGRALDTLPGALRVLNDNRTNIVDTFAALRRFATVGARILAE
                     TKDDFAADIKDLYAVIKPLNDNRAALVSDLDSLATFPFPGKNLKRVARGDYFNIFAVF
                     DLTLRRLGETIFTTSLGLDPNMKHLDEVVNPPDFLVGEMANLSGQAADPFKIPPGTAS
                     GQEVPPQ"
     gene            complement(1430..2884)
                     /locus_tag="VFC01_02950"
     CDS             complement(1430..2884)
                     /locus_tag="VFC01_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HZN78635.1"
                     /translation="MSRWLRYGIITALAVALIGGIYVVWPRVGTYTVVGYFTSATGLY
                     PGDDVRVVGVPIGSIESISPEADAVKITMRVQDSVKLPADARAVIISPNIVAARFIQL
                     APAYKQGEAMKDGGSIGLDRTAVPVEWDDVKTQLTQLSQQLGPQAGQVQGPLGAFINQ
                     AADTFDGNGDSFRRALRELSQTAGRLGDSRTDLFGTVKNLQILIDALSNSNEQIVQFS
                     GHLASVSQVLADSSVDLDNTLGALNQALSDIRGFLDQNNEALIGSINRLTDFTSLLSN
                     QSDDLEQILHVLPNAMANFYNIYNPAQGTANGLLGLPEFANPVQFICANFDAAGTTDN
                     IKRTEICRQRMAPVLRRLMMNYPPIMAHGINTITAYKGQVIYDTPATEAKAQTYIPYL
                     EWIPADGRYPPRSGEGGDVSSLLLPPNPVPGPPPPAQPYTLGPVTLPPGPAPGEPPPP
                     PAAPGPLPAEAAALPADAVPPMQPVAPQQPGGGG"
     gene            complement(2881..3927)
                     /locus_tag="VFC01_02955"
     CDS             complement(2881..3927)
                     /locus_tag="VFC01_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN78636.1"
                     /translation="MDKTIRTGIFGIVVVACVVMVAFGYSSLPFYPQGKSYEAYFADA
                     GGISPGNDVNVSGIAVGKVSGVALAGDVAKVTFTVNRKVRLGDQTLASIKTDTVLGER
                     SLAVTPQGGGSVTSIPLGRTTVPYTLNMALQDLGQNASELDKGQLNQALGVLTDSLRD
                     ATPELRRTLDGVAALSRSINANDEALGQLLARAKSVTKVLADRAGQVNQLINDGNLLF
                     AALDERRAALSNLIAGIDEVSQQISGFVADNRREFGPALRKLNLVLDNLLERREHISQ
                     ALRRLPPYATALGESVGSGSGFSVSLPNSLPNIPQAELLLDLYFQPGKVPDSLADFLR
                     GFISERLIVRPKSP"
     gene            complement(3920..4954)
                     /locus_tag="VFC01_02960"
     CDS             complement(3920..4954)
                     /locus_tag="VFC01_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN78637.1"
                     /translation="MGHRGLLIKVSIFAVAMLLVSAGLVVIFGEFRFASTNTYHATFS
                     DAARLKGGNDVRIAGVPVGSIKDVKLNPDNTVDVAFTVNKKYQMYTSTRAVIRYENLV
                     GDRYMEVTSGPGELRKLPAGGTVDIDHTQPALDLDALLGGLRPVVKGLNGEQVNQINN
                     AILELLQGQGGALSQLLSETGTFSQTLADRYQVISEVIHNLNAVLGTVDEKSAQFDAS
                     VDQLQQLVSGLAQGRDPIAGAIPPLASAQADLTDMLVNSRRPLQGVLENLRPLATELD
                     NRKDEVNRVIEPLAENYLRLNALGSYGAYFNYYICAVSLKFNGPAGSDIMLPMGGVPD
                     PSKGRCSDNG"
     gene            complement(4954..6144)
                     /locus_tag="VFC01_02965"
     CDS             complement(4954..6144)
                     /locus_tag="VFC01_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN78638.1"
                     /translation="MSRTTAIRIAAAVLAAIIVVFLVFTYLAYSAAFTPTDTVTVTSP
                     RAGLVMDKEAKVKYRGIQIGKVEDISYTGDQAKLTLAINRDQLRYVPSNALVRIGSTT
                     VFGAKSVEFIAPEQPTGTRLRPGATVQAEAVQLEANTLFQTLMDVLNKVDPVHLNATL
                     SAIAEGLRGHGDDFGATLAGLNQYLAQLNPKLPTVESDFAQTATVANIYGDAGPDLVT
                     VIDNVPTISNTIVDQQENLNATLLAATGLANEGSATLEPGADNYIAAIQRLRAPLKVL
                     GDYSPEIGCILLGVKNGADRAGRLVGGIKPGAMVSSSFVLGVPSYTYPESLPIVNASG
                     GPNCRGLPDLPSKQYGGSWYRAPFLVTDNAYIPYEPFTEVQVDAPNTLQFLFNGAFAE
                     RDDF"
     gene            complement(6156..7010)
                     /locus_tag="VFC01_02970"
     CDS             complement(6156..7010)
                     /locus_tag="VFC01_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN78639.1"
                     /translation="MSELGSYDITLRLRRAFRWLPRAVDAFGEQALFYAESIRYMPLA
                     LRRYRKETVRLIAEMTLGTGALILIGGTVGVAAFLTLASGGVIAVQGYESLGNIGIEA
                     LTGFLSAFLNVRIVAPIIAGIALAATIGAGTTAQLGAMRIAEEIDATEAMAIHSVSYL
                     VSTRIMAGLVAIIPLYSLAVLASFFSARAITVYVNGQSGGLYDHYFNTFLIPTDLLWS
                     FAQAIVMSIAVMLVHTYYGYNASGGPVGVGIAVGQAVRTSLIVVVVITLFIALAVYGA
                     SGNFNLSG"
     gene            complement(7007..7771)
                     /locus_tag="VFC01_02975"
     CDS             complement(7007..7771)
                     /locus_tag="VFC01_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN78640.1"
                     /translation="MREQLAVPARAVGGFMEMSIDTFRAMFRRPFQFGEFLDQTWMIA
                     RVSIIPTLLISIPFTVLVAFTLNILLREIGAADLSGSGTAFGTVTQLGPVSTVLVVAG
                     AGATAICADLGARTIREEIDAMRVLGIDPVQRLVVPRVLASTLVALLLNGLVIAIGLS
                     GGYVFSVLLQGVNPGAFINGLTILTGLGELVVSEVKALLFGVMAGLVGCYRGLTVKGG
                     PKGVGEAVNETVIYAFICLFVINVLMTAIGVRVLGR"
     gene            complement(8034..8954)
                     /locus_tag="VFC01_02980"
     CDS             complement(8034..8954)
                     /locus_tag="VFC01_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HZN78641.1"
                     /translation="MTSISDATDLSGLVAVVTGAAAGLGRAEAIGLARAGATIVVNDI
                     AGTLDKSDVIDEITSAGAKAVAVAGDISQRSTADELVETADGLGGLSIVVNNAGITRD
                     RMLFNMTDDDWDAVMAVHLRGHFLLTRNAATYWRAKAKEGDGTVYGRIINTSSEAGLA
                     GPVGQANYGAAKAGITALTLTAARALERYGVRANAIAPRARTAMTADVFGDSPELEDG
                     QIDPLSPEHVVNLVRFLASPASKDVNGQLFIVYGPTVTLVAAPTAEHRFSAESDGWNP
                     SELSKTLQNYFADRDPERNFSATALMGQGE"
     gene            complement(8968..9159)
                     /locus_tag="VFC01_02985"
     CDS             complement(8968..9159)
                     /locus_tag="VFC01_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN78642.1"
                     /translation="MRVEVDRDRCEGNAVCVGIAPDLFDLDDEDYAVVKADPVPADQE
                     DLAEQSIAECPRAALIRRD"
     gene            9300..10484
                     /locus_tag="VFC01_02990"
     CDS             9300..10484
                     /locus_tag="VFC01_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN78643.1"
                     /translation="MRIGYTPEQEELRRELRAYFTKLMTPERAEALAAGDGGEMGRGN
                     VYRETVAQMGKDGWLTLSWPKEFGGQARPPMDGLIFNDEAAIANVPVPFLTINSVAPT
                     IMAFGTDEQKKFFLPKIAAGDLHFSIGYSEPGAGTDLAALRTTAVRDGDDYVINGQKM
                     WTSLIAYADYVWLAVRTNPEAKKHRGISMLIVPTTAEGFSWTPVHTMAGVDTSATYYQ
                     DVRVPVGNLVGEENGGWKLVTNQLNHERVALVSAQPIFLALNGVREWAQNTKDVHGNR
                     LIDSEWVQLNLARVHAKAEVLKLINWELASTEDAAPSPADASAAKVYGTELATEAYRL
                     LMEVLGTAGTLRTDSPGALLRGRVERMHRSCLILTFGGGTNEIQRDIIGMVALGLPRV
                     NR"
     gene            10493..11593
                     /locus_tag="VFC01_02995"
     CDS             10493..11593
                     /locus_tag="VFC01_02995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN78644.1"
                     /translation="MDFSTTEAANDLGGLTRTITEAVCTNEHQRELDSLDEKFDRPLW
                     GKLIESDILSAATPEALGGGGFGVLEQVAVLVALGRQLAAVPYLESVVLGAGALAKFG
                     SEALQQEWATPAITGEKIIALALDGEMGQGPVQATSSGAAYKLTGSRTQVGYGPVADA
                     FLVPAETDSGRNVFLIAKDDPGVTVTSLDTTGHGSVGHLELQGVEAARVVGGDQGDTV
                     LAWLTNHSSLGRSAYQLGVLERALELTSQYAGEREQFDRPIGSFQAVSSRLADGYIDV
                     KALRLTVTQAAWRLSEDLPADVDVDTAAFWAADAGHRVAHTTVHVHGGVGIDLDHPVH
                     RYFLAAKQTEFAVGGATGQLLRIGRELADTPA"
     gene            11597..13102
                     /gene="fadD17"
                     /locus_tag="VFC01_03000"
     CDS             11597..13102
                     /gene="fadD17"
                     /locus_tag="VFC01_03000"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD17"
                     /protein_id="HZN78645.1"
                     /translation="MTGSLPTVTDLLAPLVEVDDRGVYFEDSFTSWRDHIRHGAAVAA
                     VLKARLDPDKPPHVGVLLQNTPFFSEVLVGAALTGIVPVGLNPVRRGAALARDVTHAD
                     CQLVLADSASATGLDVDYLDVDSPEWAAEVAALRDEPVAAYDADPSDLFMLIYTSGTS
                     GEPKAVNCSQGKVAIAGVTMTQRFSLGSNDICYVSMPLFHSNAVLVGWAVALACGGSL
                     ALRRKFSASQFLPDVRRYGATYANYVGKPLSYVLATPERPDDADNSLRAVYGNEGAPA
                     DVDRFAQRFDTVVMDGFGSTEGGIAIGRTPDTPPGALGPLPPGTEIVDVDTGEPCPPG
                     VTGELVNASDAGRFEGYYNDPDAEAERMRGGMYHSGDLAYRDENGYAYFAGRLGDWMR
                     IDGENLGSAPIERVLLRHPDVVEVAVYGVPAPDVGDQVMAALVLSEGAEFDAGKFKAF
                     LAEQPDLGPKQWPSFVRVGAELPRTETFKVIKRQLRSEGTDCADPVWPIER"
     gene            13139..13867
                     /locus_tag="VFC01_03005"
     CDS             13139..13867
                     /locus_tag="VFC01_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZN78646.1"
                     /translation="MGYREFAPPAALRDLVECAWVVDGPAEPVRVLPDGCMDLMRMDG
                     RIMIAGPDTAASISPRDAEPFVGLRFHPGVLPRLLRVPASELRNARVPLRDVQRGARQ
                     HGSLTEIAAALASEEPQAETAPWSLQLLGHVTGRLAAGSTVAELAQQVGWSARTLQRQ
                     CTAVYGYGPAMLRRILRFRRAVRLLDDGLSLGEVAAGAGYADQPHLHREARELAGVPL
                     ASLRQDSSSANRSTQFPSGSATVA"
     gene            complement(13804..14202)
                     /locus_tag="VFC01_03010"
     CDS             complement(13804..14202)
                     /locus_tag="VFC01_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN78647.1"
                     /translation="MDIRLTSAVFQVFAHDLARSIAFYRLLGLPVPDAEIPHVAVELP
                     GGNSLSLDTDETIAGMHPGWSPPSSSDSRVALALGVSSPAEVDALFEKITAAGHAGQL
                     APYDAPWGMRYATVADPDGNWVDLFALLES"
     gene            14424..15137
                     /locus_tag="VFC01_03015"
     CDS             14424..15137
                     /locus_tag="VFC01_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqN/LpqT family lipoprotein"
                     /protein_id="HZN78648.1"
                     /translation="MKRSAITGTAAIAAALAVLLSGCGSDTKTEPSTTTSASKSTSTS
                     AKAAPSWKPKVAPRDEDAKGPHPTIASYIQENNIQETPVKRGDPGSPTIDLPIPDGWA
                     PGGEDTPEWAYGAIVYTGPDAGDYTPSIVALVSKLTGNVDPQQIIDLAPGELNNLPGW
                     KAMNEGEASTLGEYPAYQLGGTWVQDGQTKIVAQKTVVIPGSDGLYVLQLNADGLEDQ
                     KEIIGAATDVIDSQTTITP"
     gene            complement(15208..15777)
                     /locus_tag="VFC01_03020"
     CDS             complement(15208..15777)
                     /locus_tag="VFC01_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN78649.1"
                     /translation="MAQTAVRRMARAERREHLLDAATRAFARSGFAGTSLDNIAAEAG
                     VTHVILYRHFDSKSDLYRAVLERAAARLSEIVGTDEFDEGSISRLLRGAAGDPDGFRL
                     LFRHAVREAEFRDIVDEIRSTAVDIANRQLADRINEGQWRQWASRLLPTLTLEAVIAW
                     LDAGQPDPDRAADHIGHAVEAVIQAASDI"
     gene            15924..17033
                     /locus_tag="VFC01_03025"
     CDS             15924..17033
                     /locus_tag="VFC01_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN78650.1"
                     /translation="MQANGSVHRVRTEVGDPAWLITGHATVRRLLEDDRLGRSHPQPE
                     AAARAGESVLFGGPTGNFATERADHTRMRTLLQPHFTPKHMRALTGRIEALTTQLLDT
                     MAEQGPPVDLHTAVALPLPILVICELLGVPYEDRDQFRRWTTEASNTRDRTLSAHGVW
                     QLFGYGQRLVAAKRHQPGDDVISRLCATKGVPDDEIAWLSMALLFAGHETTVVQIGLA
                     TLLLLAQREQWQALVNDPGLVSRAVEETLRSSRKGGGGTIPRYASADLEVDGVTIKTG
                     ELVLIDIAAANQDPVAFADPDRVDITRSSLAHLTFGYGPRYCLGAPLARVELQVALSQ
                     LASRFPTLRLAVEVDELTMRADVLVGGLTELPVRW"
     gene            complement(17041..18162)
                     /locus_tag="VFC01_03030"
     CDS             complement(17041..18162)
                     /locus_tag="VFC01_03030"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HZN78651.1"
                     /translation="MRTELCDRFGIDYPIFVFTPSEKVAAAVSKAGGLGVLGCVRFNE
                     AEDLENVLCWMDENTDGKPYGVDIVMPAKVPQEGTAVDIDKLIPQSHRDFVAKTLADL
                     GVPPLPDEGERNEGVLGWLHSVARSHVEVALKHPIKLIANALGSPPKDVIDQAHETGV
                     PVAALAGSAKHAQRHVENGVDIVVAQGHEAGGHTGEIGSMVLWPEIVDALEGRAPVLA
                     AGGIGTGRQVAAALALGAQGVWMGSAFLTSAEYDLGHRKPSGVSTIQEALLRANSSDT
                     VRRRIYTGKPARLLKSRWTDAWDAPDAPEPLPMPLQNILVSEAHQRMNESDNPDTVAM
                     PVGQIVGRMNEIRPVADIIAELVAGFEDATRRLDGIREN"
     gene            complement(18162..>19268)
                     /locus_tag="VFC01_03035"
     CDS             complement(18162..>19268)
                     /locus_tag="VFC01_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN78652.1"
                     /translation="MWRHEDIYRVLFGGTDFATGEFVKDEYDLAKAAAENPPMIRYPI
                     PPMIHGATQSATWMSLFSGQTTVLAPEFDADEVWATIEKYKVNLLFFTGDAMARPLLD
                     ALTKLHDGGHEPDLSSLFLLASTAALFSTSIKEKFLELLPNRVITDSIGSSETGFGGT
                     SIVAKGQSHTGGPRVTIDHRTVVLDEDGNEVKPGSGVRGMIAKKGNIPVGYYKDEKKT
                     AETFKTFNGVRYAIPGDYAEVDEDGSVTMLGRGSQSINSGGEKIYPEEVEAALKGHPD
                     VFDALVVGVPDERYGNCVAAVVHRRPGTNPTLAELDTFVRQEIAGYKVPRKVWWVDEI
                     HRTPAGKPDYRWAKDQTEERPADDAHANHVGTSS"
BASE COUNT         3420 a         6237 c         6219 g         3392 t
ORIGIN      
        1 gacgccgtcg tcggtcagct tcacggactc aacgcgtccg gcctgcacgc cgcggaaggt
       61 gacattggca ttctcataaa ggccgccgcc ggccacgaag ttcgcagtga cctgatacgc
      121 accgattccc agccgggccg ggacgcgcag gtagaacagc gagatcgcgc cgacggtgag
      181 cacagtcacg acggcgaaga ccaccaattg gattcgcgca gttcggttca gcattactgt
      241 ggcggcacct cctgacccga cgcggtccct ggcgggatct tgaacggatc tgcggcctgc
      301 ccagagaggt tcgccatctc cccaaccagg aagtccggcg ggttgacgac ttcgtcgagg
      361 tgcttcatgt tcgggtcaag gcccaacgac gtcgtgaaga tcgtctcgcc gagccggcgg
      421 agcgtcagat cgaacacagc gaaaatattg aagtagtcgc ccctggccac gcgcttcaaa
      481 ttcttacccg ggaacggaaa ggtcgccagg ctgtccaggt cggagacgag ggccgcacgg
      541 ttgtcgttga gcggcttgat gaccgcgtaa aggtccttga tgtctgcggc gaaatcgtcc
      601 ttcgtctccg caaggatgcg tgcccccacc gtggcgaacc tccgcaacgc cgcgaacgtg
      661 tcgacgatgt tggtccggtt gtcgttgagc acccggagcg ctccgggaag cgtatccagg
      721 gcacggccca gactgtccct ctggcgcgcg aggactccgg cgaaccggtt caggccttcg
      781 gctgcggcga tgatgtcgtc ggtctgccga ttcaacgacg cggccagctc ggcgagtcgc
      841 ggaatcaggt cagcgaactg gccctgacgc ccggccaccg cgttggagac ctcttgggtg
      901 atgtcttgca gcgcaccgag attgcccttg ttgacgacca cacccaacgc ggaaagtact
      961 tcctccgtgg tcgggtagcg gccgccctgg ctttgcggga tctccgaccc gtcgcgcagc
     1021 tcgccaaccg ctggctggcc caccggagcc gatagctgga tatgctgcga ccccagtagt
     1081 gatgtctgcc cgaccttcgc cgtcgcattg gccggcaact tgacgttggg ttccagggac
     1141 actttcaccg ccgcgtagaa cgtgccatcc ggccgctgca ccgcctccac ccccgagacg
     1201 ctgccgacgg tgacgtcatc aaccagcacc ggcgaattct gcggcagggt ggccacgtcc
     1261 ggcagctcca cggtgattga gtaagcgccc ggaccgtgac cggcggtgcc cggcatattc
     1321 agcgagttca acccgccgaa ctggcagccg gcaagaagta ccacgccgga accgatggcc
     1381 accgtgcggc tcgttgagcg gcccacgttg atacccaacg actttcgagt catcctccgc
     1441 ctcccggttg ttgcggtgcg accggctgca tcggcgggac ggcatcggcc gggagcgcag
     1501 cagcctcggc cgggagtggg ccgggcgcag ctggaggtgg cggcggctca ccaggcgctg
     1561 gtcccggcgg cagtgttacc ggaccgagcg tgtacggctg cgccggtggc ggtggacccg
     1621 gaaccgggtt cggtggcagg agcagcgaac tcacatcccc gccctcgccg gacctaggcg
     1681 ggtaccgccc gtccgccggt atccactcga gatacgggat gtaggtctgc gccttggcct
     1741 ccgtcgccgg ggtgtcatag atgacctggc ccttgtacgc ggtgatcgtg ttgatcccgt
     1801 gcgccatgat gggcgggtaa ttcatcatca ggcgccgcag caccggagcc atccgctggc
     1861 ggcatatctc ggttcgcttt atgttgtcgg tcgttccagc ggcgtcgaag ttcgcgcaaa
     1921 tgaactgcac cgggttggcg aactccggca ggcccagcag accgttcgcg gtgccctggg
     1981 ccgggttgta gatgttgtag aaattcgcca tcgcgttcgg gaggacatgc agtatctgct
     2041 cgaggtcgtc gctctggttc gacagcaggc tcgtgaagtc ggtcagcctg ttgatgctgc
     2101 cgatcagcgc ctcgttgttc tggtctagga aaccgcggat gtccgaaagg gcctggttga
     2161 gggcgcccag cgtgttatcc aggtccaccg agctgtccgc cagcacctgc gacaccgagg
     2221 ccaaatgccc actgaattgg acgatttgct cgttgctgtt cgacagcgcg tcgatcagga
     2281 tctgcaaatt cttcacggtg ccgaacaggt cggtgcgtga gtcacccagc cgtcccgcgg
     2341 tctgcgacaa ctcgcgtagt gctcggcgga acgagtcgcc gttgccgtcg aaggtatcgg
     2401 cggcctgatt gatgaacgcg cccaatggac cctgaacctg gcccgcctgc ggtccgagct
     2461 gctggctgag ctgagtcagc tgcgttttga catcgtccca ttccaccggc acggccgtgc
     2521 ggtccaaccc gatactgccg ccgtccttca tcgcctcgcc ctgcttatac gccggggcga
     2581 gctgaatgaa ccgggccgca acgatgttcg gcgagatgat gaccgcgcgg gcgtcggcgg
     2641 gcagtttgac actgtcctgc accctcatcg tgattttgac ggcatcggcc tcgggcgaga
     2701 tcgattcgat cgagcctatc ggtacaccga cgacgcggac atcgtcgccc ggatacaagc
     2761 cggttgccga cgtgaaatat ccaaccaccg tgtatgtgcc aacccggggc cacaccacgt
     2821 aaatgccgcc aatcagcgcc accgccagag cggtgatgat tccgtaacgc aaccagcggc
     2881 tcatggcgat ttcggcctta cgatcaaccg ctcggagatg aatccgcgca ggaaatcggc
     2941 caggctgtcc ggaaccttgc ccggctggaa gtagaggtcc aacagcaact ccgcctgcgg
     3001 aatgtttggg agcgagttgg gcaggctgac gctgaatccc gaaccggagc cgaccgactc
     3061 gccgagggca gtggcatagg gcggcaatcg ccgcagcgcc tggctgatgt gctcccggcg
     3121 ttcaagcagg ttgtccagga cgaggttcag cttcctcagg gccggaccga actcgcggcg
     3181 gttgtcggcc acaaatcccg aaatctgctg tgacacttca tcgatgccgg caatcaagtt
     3241 actcagcgcg gcacggcgct cgtcgagcgc agcgaatagt agattaccgt cgttgatgag
     3301 ctggttcacc tgaccggccc ggtcggcgag caccttggtc accgacttcg cgcgcgccaa
     3361 cagctggccc aacgcctcgt cgttggcgtt gatgctgcga gacaacgcgg ccaccccgtc
     3421 gagtgttcga cgcagctcgg gggttgcgtc gcgaagcgaa tcggtcagca cacccaacgc
     3481 ctggttcagc tgacctttgt cgagttcgct ggcgttctga ccgagatcct gcagcgccat
     3541 gttcaacgtg tacggaactg ttgtgcggcc caatggaatt gatgtcaccg agccgccgcc
     3601 ttgtggggtc accgccagcg acctctcacc caggacggtg tcggtcttga ttgatgccag
     3661 cgtttgatcg cccagcctga ccttgcggtt gacggtgaag gtcaccttcg cgacgtcgcc
     3721 tgcgagcgcg accccgctca ctttgccgac cgcgatgccg gagacgttga cgtcgttacc
     3781 cggcgagatg ccaccggcgt ctgcgaagta ggcctcgtac gacttgcctt gcggataaaa
     3841 cggcaggctg ctgtagccga acgcgaccat cacgacacac gcgacgacca cgattccgaa
     3901 gatgcccgtc cggatcgtct tatccattgt ctgaacacct gcccttcgac gggtccggga
     3961 ctccgcccat tggcagcatg atgtcgctgc ccgctggccc gttgaacttg agcgagacgg
     4021 cgcagatgta atagttgaag tacgccccat aagagccaag ggcgttgagc cgcaagtagt
     4081 tttcggcgag cggttcgatg acccggttga cctcatcctt gcggttgtcg agctcggtgg
     4141 ccagtgggcg aaggttctcc agcaccccct gtagcggccg ccgtgagttc accagcatgt
     4201 cggtcagatc cgcttgcgcg gacgccagcg gcggaatggc ccctgcaatc gggtcgcggc
     4261 cctgcgccag cccgctgacc aactgctgga gctggtccac gctggcatcg aactgggcgc
     4321 tcttctcgtc aacggtccca agcaccgcgt tgagattgtg gatcacctcc gagatcacct
     4381 gatagcgatc ggccagggtt tggctgaatg ttccagtctc cgagagcaat tgcgacagcg
     4441 cgccgccctg gccctgcaac agctcgagga tcgcgttgtt gatctggttg acctgctcgc
     4501 cgttgaggcc cttgaccacc gggcgcaggc caccaagcaa cgcatcgaga tccaacgccg
     4561 gctgggtgtg gtcgatgtcg accgtgccgc ccgccggcag cttccgaagc tcgccggggc
     4621 ccgaggtgac ttccatgtag cggtcgccga ccagattctc gtagcggatc acggcacgcg
     4681 tcgacgtgta catctgatac ttcttgttga ccgtgaacgc cacatcgacg gtgttgtcgg
     4741 ggttcagctt cacatctttg atgcttccca ccggaacgcc ggcgatccgc acgtcgttgc
     4801 cacccttcag ccgcgcggcg tcgctgaatg tggcgtggta ggtgttggtg gaggcgaacc
     4861 ggaactcgcc gaagatcacc accaaaccag cggagaccaa gagcatcgcc accgcgaaga
     4921 tgctcacctt gatcaggagt cccctgtggc ccattagaag tcgtcccgtt ctgcgaatgc
     4981 gccattgaag aggaactgca gggtattcgg tgcatcgacc tgcacctcgg tgaacggctc
     5041 gtacggaatg taggcattgt cggtaaccag gaatggtgcc ctgtaccacg agccgccgta
     5101 ctgcttgctg ggaaggtcgg gcagtccgcg gcagttgggt ccgccggacg cgttcacgat
     5161 cggcaggctc tctggatacg tgtacgacgg cacgcccagc acgaagctgg aactcaccat
     5221 cgcgcccggt ttgatgcccc cgaccagacg tcccgcccgg tcggcgccgt tcttgacgcc
     5281 cagcaatatg cagccgattt ccggcgaata gtcgccaagc accttcaacg gcgcccgcaa
     5341 ccgctggatg gccgcgatgt aattgtctgc cccgggctcc agcgtggcgg atccctcatt
     5401 cgccaagccg gttgccgcga gcagcgtcgc gttcaagttt tcctgctgat cgacgatcgt
     5461 gttgctgatc gtcggcacat tgtcgatcac agtgacaaga tcgggaccgg cgtccccgta
     5521 gatgttggcc accgtcgctg tctgcgcgaa atccgactca accgtcggca actttggatt
     5581 cagttgcgcg agatactgat tcaggcccgc cagggtagcg ccgaaatcgt cgccatgacc
     5641 acgcaggccc tcggcgatcg ccgacagagt cgcgttcagg tgcactggat cgaccttgtt
     5701 cagcacgtcc atcagggtct ggaacaacgt gttggcttcc agctgcacgg cctcagcctg
     5761 gaccgtggcg ccgggacgca accgtgtgcc cgtcggctgc tccggcgcga tgaactccac
     5821 cgacttcgcg ccgaacactg tggtgctgcc gatgcgcacc agagcgttcg aggggacgta
     5881 gcgcagctgg tcgcggttga tcgccagcgt caacttcgcc tgatcaccgg tatacgagat
     5941 gtcttcgacc ttgccgatct gaataccgcg gtacttgacc tttgcttcct tgtccatcac
     6001 caaaccggcc cgcggcgatg tcaccgtcac ggtgtcggtc ggggtgaacg cggcggagta
     6061 cgccaagtag gtaaatacca agaacaccac gatgatcgcc gccaacactg cggcggcgat
     6121 cctgatcgcg gtagtcctcg acacgacgtt cccctctacc ccgacaggtt gaaattaccg
     6181 gacgcgccgt atacggcgag ggcgatgaac aaagtgatga cgaccacgac gatcagcgac
     6241 gtccgcaccg cttggcccac agcgataccc actccgacgg gtccgccgga ggcgttgtag
     6301 ccgtagtacg tgtgcaccag catcaccgcg atcgacatca cgatggcctg ggcgaacgac
     6361 cacagcaagt ccgttggtat caggaacgta ttgaaatagt ggtcatatag accgccggat
     6421 tgtccgttga cgtagacggt gatggcgcgg gcagagaaga acgaggccaa cacggccagc
     6481 gagtacagcg gaatgatcgc gaccagcccg gccatgatgc gggtggacac caggtaggac
     6541 accgaatgta tggccatcgc ctcggtggcg tcgatctcct cggcgatgcg catcgcgccg
     6601 agttgggcgg tggtaccggc gccgatggtg gcggcgagcg caatgccggc gatgatcggc
     6661 gcgacgatgc gtacgttgag aaaggcggag aggaacccgg tcagcgcctc gatgccgatg
     6721 ttgccgagcg actcgtagcc ctgcaccgcg atgacgccgc cggatgccaa ggtcaggaac
     6781 gccgcgacgc cgaccgtgcc gccgatgagg atcagcgcac cggtgcccag agtcatctcg
     6841 gctatcagcc ggaccgtttc cttgcgatag cgccgcaacg cgaggggcat gtatcggatt
     6901 gactcggcgt agaacaacgc ctgctcgccg aaggcgtcga cggcgcgggg cagccatctg
     6961 aacgcccgcc gcagccgcag cgtgatgtcg taactcccca gttcgctcat cggcccaaga
     7021 cccgcacgcc gattgccgtc atgagcacgt tgatcacgaa caggcagatg aaggcataga
     7081 tgacggtctc gttgacggct tctccgacac ccttcggtcc acccttgacg gtgaggccgc
     7141 ggtagcagcc gacgagcccc gccatgacgc cgaacagtag ggccttgact tctgaaacca
     7201 ccaactcgcc caacccggtc aggatggtca agccgttgat gaacgcccct gggtttaccc
     7261 cctgcagcag caccgagaat acgtagccgc cgctcaggcc tatcgcgatc accagaccgt
     7321 tgagcagcag tgccactaac gtcgacgcca ggacgcgcgg caccacgagc cgttgaacag
     7381 gatcgatgcc gagcacccgc atcgcgtcga tttcctcacg gatggtgcgg gcgcccaggt
     7441 cggcgcagat cgcagtcgct ccggcgccgg cgacgaccag cacggtcgaa accggaccca
     7501 gctgagtcac ggtgccaaac gcggtgcccg agccggacag gtccgcggcg ccgatctcac
     7561 gcaacaggat gttgagggtg aacgcgacca gcactgtgaa cggaatcgag atcagcagcg
     7621 tcggaatgat cgaaaccctg gcgatcatcc aggtctggtc gaggaactcg ccaaattgga
     7681 aggggcgacg gaacatggcc cgaaaagtat cgatggacat ttccatgaac ccgccgacgg
     7741 cccgagccgg aacggcgagc tgctccctca acctcggctc cgttctcggc gcgcacggcg
     7801 cgagatgtgc gccatggcga agagtgcgag actacttgcc gcggcggttc tcccacccgt
     7861 tacttaagcg ttgctcttag tgagccggat catactaact agaacatgtt cgcagtgtca
     7921 aagaacagca aaactacccg aaaccgtaat ctttttcctg gtcagcgaca ctgtgaccca
     7981 ggtcattctg taacacgttc tagtcgatgc aggactctga caatgagaga acgtcattct
     8041 ccttgaccca tgagggccgt cgcggagaaa ttacgttcgg gatcacggtc agcaaagtaa
     8101 ttttgcaacg ttttcgacag ttctgacggg ttccagccat cggattccgc actgaaccga
     8161 tgctcggccg tcggcgcggc aacgagcgtc accgtcggcc cgtaaacaat gaacagctgt
     8221 ccgttgacat ctttcgatgc gggggaagcg aggaaccgca ccagattcac gacgtgctcc
     8281 ggcgatagcg gatcgatctg accgtcctcc agctcgggcg aatccccgaa cacgtcggcc
     8341 gtcatcgcgg tgcgcgcacg aggagcgatg gcgttggccc gcaccccgta acgctccagc
     8401 gcacgcgcgg ccgtcagcgt cagcgcagtg atgcccgcct tggcggcacc gtagttggcc
     8461 tgcccaactg gtccggccag tccggcttcc gaggacgtgt tgatgatgcg gccgtagacg
     8521 gtgccgtcac cctctttagc cttcgcccgc caataggtgg ccgcgttacg cgtcaacagg
     8581 aagtggccgc gcaggtgcac cgccatcacc gcgtcccagt cgtcgtcggt catgttgaac
     8641 aacatgcggt cgcgggtgat ccccgcgttg ttcacgacga tgctcaggcc gccgaggccg
     8701 tcggccgttt ccaccaattc gtcggccgtg gaccgctggc tgatgtcgcc cgcgacggcg
     8761 acggccttgg caccggcgct ggtgatctcg tcgatgacgt cggatttgtc gagcgtgccc
     8821 gcgatgtcgt tgaccacgat ggtcgcgccc gcccgagcaa gcccgatggc ctcggcgcgg
     8881 cccaggcccg cggccgcacc ggtcaccacc gcgaccagtc cggacagatc ggtcgcgtca
     8941 ctaattgaag tcaatttatg aatacctcta gtctctgcgg atcagggcag cgcgggggca
     9001 ctcggcgatc gactgttcgg ccaggtcctc ctggtcggcc ggcaccggat cggccttgac
     9061 cacggcgtaa tcctcgtcgt cgagatcgaa caggtccggg gcgattccca cgcacaccgc
     9121 gttcccctca cagcggtcgc ggtccacttc cactcgcatc acaacctcct cgcggtaaga
     9181 gccgctctga gcaccagtgt ggcaccggcc tggaccccaa gactagaacg tgttacaacc
     9241 gggagtgaaa gcgggcccag acgacggttt cttatacctg tagaagttag gacaaggcga
     9301 tgcggatcgg ctacaccccc gagcaggagg agttgcgccg cgagctgcgc gcgtacttca
     9361 ccaaactcat gacgcccgag cgcgctgagg cgctggccgc gggcgacggt ggcgagatgg
     9421 ggcgcggcaa cgtctaccgc gagacggtcg cacagatggg caaggacggc tggctgacac
     9481 tgtcgtggcc caaggagttc ggcggccagg cacgcccgcc gatggacggg ctgatcttca
     9541 acgacgaggc agccattgcc aacgtgccgg tgccgttcct gaccatcaac agcgttgcgc
     9601 cgacgatcat ggcgttcggc actgacgagc agaagaagtt cttcctgccc aagatcgccg
     9661 cgggtgacct tcacttctcg atcggctact ccgagcccgg cgcgggtacc gacctcgccg
     9721 cgctgcgcac taccgcggtg cgcgacggcg acgactacgt catcaacggc cagaagatgt
     9781 ggaccagcct gatcgcctac gcggactacg tgtggctggc agtgcgcacc aacccagaag
     9841 ccaagaagca ccgcggtatc tcgatgctga tcgtgcccac gacggccgag ggcttctcct
     9901 ggacgccggt gcacaccatg gcaggcgtgg acaccagcgc cacctactac caggacgtcc
     9961 gggtgcccgt cggcaatctg gtcggcgagg agaacggtgg ctggaagctg gtcaccaacc
    10021 agctcaacca cgagcgcgtc gcgctggtct cggcgcagcc gatcttcctg gcgctcaacg
    10081 gcgttcgcga gtgggcacag aacaccaagg acgtgcacgg caatcgactg atcgattccg
    10141 agtgggtgca gctgaacttg gcccgcgtgc acgccaaggc cgaggtgcta aagctgatca
    10201 actgggaact ggcatccacc gaggatgcgg ctccgtcacc cgccgacgcc tcggcggcca
    10261 aggtgtacgg caccgaactg gccaccgagg cctaccgtct gctcatggag gtgctgggca
    10321 cggcgggcac gctgcggacg gactcccccg gcgcgttgct gcgcggacgc gtcgagcgga
    10381 tgcaccgcag ctgcctgatt ctgacgttcg gcggcggcac caacgagatt cagcgcgaca
    10441 tcatcggcat ggtcgcgctc ggcctgcccc gggtcaatcg gtaagggaca tcatggattt
    10501 ttcgactacg gaagccgcca acgatctggg cggcctgacc cggaccatca cagaagccgt
    10561 ctgcaccaac gaacaccagc gggagctcga cagccttgat gagaagttcg accgccccct
    10621 gtggggcaag ctgatcgagt ccgacatcct gtcggccgcc acgccggaag cgttgggcgg
    10681 cggcggtttc ggcgtgctcg aacaggtggc ggtgctggtg gcgctcggcc gtcagctcgc
    10741 cgcggtgccg tacctggagt cggtggtact cggcgcgggc gcgctggcga agttcgggtc
    10801 ggaagcgctg cagcaggagt gggcgacgcc ggcgatcacc ggcgagaaga tcatcgcgct
    10861 ggccctcgac ggcgagatgg gccagggtcc ggtgcaggcg accagtagcg gtgcggcgta
    10921 caagctgacg ggctcacgaa cgcaggtcgg ttacggaccg gtcgccgacg cgttcctggt
    10981 tccggccgaa accgattcgg gcagaaacgt tttcctgatc gcgaaggatg atcccggcgt
    11041 gacggtgacg agcctcgaca cgaccggaca tggcagtgtc gggcatctgg agttgcaggg
    11101 agtggaggcc gctcgggtcg tcggtggcga tcagggcgac acggtgctcg cctggctcac
    11161 caaccacagc agtctcggcc gcagcgcata tcagctcggg gtcctcgagc gtgcacttga
    11221 gctcacgtcg cagtacgccg gcgagcgtga gcagttcgac cggccgatcg gcagcttcca
    11281 ggccgtgtcg tcgcggctgg ccgacggcta catcgacgtc aaggcactcc ggctgaccgt
    11341 gacccaggcg gcgtggcggt tgtcggagga tcttcccgcc gacgtcgacg tcgacacggc
    11401 ggcattctgg gcggccgacg ccggacatcg cgtggctcac accaccgttc atgtgcatgg
    11461 cggggtcggg atcgacctcg accacccggt gcaccggtac ttcctcgcgg ccaagcaaac
    11521 ggagttcgcg gtcggcggcg ccacggggca gctgctgcgc atcggccgtg aattggctga
    11581 cacgccagcc tgatccgtga ctgggagcct gccgaccgtc accgacctgc tggcgccatt
    11641 ggtcgaggtc gacgatcggg gcgtgtactt cgaggattcg ttcaccagct ggcgtgacca
    11701 catccggcat ggcgcagcgg tggcggcggt gttgaaggcg cgtctagatc ctgataagcc
    11761 tccgcatgtc ggcgtgctgc tgcagaacac cccgttcttc tcggaagtgc tcgtcggtgc
    11821 ggcgttgacg ggcatcgtcc cggtggggct gaacccggtt cggcgcgggg ccgcgctggc
    11881 gcgcgatgtc acacacgcgg attgccaact ggtgctggct gattcggcat cggccacggg
    11941 cctcgatgtc gactacctcg acgtcgactc gccggagtgg gcggccgagg tggcggccct
    12001 tcgggacgag ccggttgctg cttatgacgc cgatcccagc gacctgttca tgctgatcta
    12061 cacctccggc accagcggcg agcccaaggc ggtgaactgc agccagggca aggtggcgat
    12121 cgccggtgtg acgatgacgc aacgcttctc gctcgggtcc aatgacatct gttacgtgtc
    12181 gatgccgctg ttccattcca atgcggtgct ggtcggctgg gcagtggcgc ttgcgtgcgg
    12241 cggctcgctt gcgttgcggc gcaagttctc cgcgtcgcag ttccttcccg atgtgcgccg
    12301 ctacggcgcg acgtatgcga actatgtcgg caagcccttg tcctatgtgc tcgccacacc
    12361 cgaacgtcct gacgacgcgg acaattcact gcgggcggtc tacggcaacg agggcgcacc
    12421 cgccgacgtc gaccggttcg cccagcggtt cgacaccgtg gtgatggacg ggttcgggtc
    12481 gacggagggc ggcattgcga tcggccgcac gccggatacc ccgcccgggg cgctgggccc
    12541 gttgccgccc ggcaccgaga tcgtcgatgt ggacaccggc gagccctgtc cacccggggt
    12601 cacgggtgag ctggtaaacg cttctgacgc aggccgtttc gagggttact acaacgaccc
    12661 ggatgccgag gcggagcgga tgcgcggtgg catgtaccac agtggcgatc tggcctaccg
    12721 cgacgagaac ggctacgcgt atttcgcggg caggctcggt gactggatgc gcatcgacgg
    12781 cgagaacctc ggatcggcac cgatcgagcg ggtactgctg cgccaccccg acgtggtcga
    12841 ggtagcggtg tacggagtcc ccgcccccga tgtcggcgat caggtgatgg cggcgttggt
    12901 gctcagcgag ggcgctgaat tcgacgccgg gaagttcaaa gcgttcctcg ccgagcagcc
    12961 ggatctcggg ccaaagcagt ggccgtcgtt cgtgcgtgtc ggcgccgagc tgcccaggac
    13021 cgagacgttc aaggtgatca agcgccagct acggtcggag ggcaccgatt gcgccgaccc
    13081 agtgtggccg atcgagcgct aaccgcggtt gtcataggcc gccggtaacc tggccaccgt
    13141 gggctatcgc gaattcgcgc cgcccgccgc gctgcgcgac ctggtcgaat gcgcgtgggt
    13201 ggtggacggc ccggctgagc cggtgcgggt gcttccggac gggtgcatgg atctgatgcg
    13261 catggatggc cgcatcatga tcgccgggcc ggataccgct gcgtcgatca gcccccgcga
    13321 cgcagaaccg ttcgtgggct tgcgatttca tcccggtgtg cttccgcgcc tgcttcgagt
    13381 gcccgcgtcg gagctgcgca atgcgcgcgt gcccctgcgt gacgtgcagc gcggcgctcg
    13441 gcaacacggt tccctgaccg agatcgcggc cgcactcgca tcggaggaac cgcaggcaga
    13501 gacagcgccg tggtcgcttc agctgctggg ccatgtgacc ggcaggctcg cggcgggctc
    13561 gaccgtcgcc gagctggcac aacaagtcgg ctggtcggcc cgcacgttgc agcgccaatg
    13621 cacagccgtg tacggctacg gccccgcaat gctgcgtcgc atcctgcggt tccggcgcgc
    13681 ggtgcggtta ctggacgatg gactgtccct tggcgaggtg gccgcaggcg cgggctacgc
    13741 cgaccaaccc cacctgcacc gcgaggcccg cgaactcgcc ggtgtgccgt tggcgtcgct
    13801 tcgtcaggat tccagcagtg cgaacaggtc cacccaattc ccgtccgggt cggcgaccgt
    13861 cgcatagcgc atgccccacg gcgcgtcata tggcgccagc tgcccggcat gccctgcggc
    13921 cgtgattttc tcgaacaacg cgtccacctc cgcgggagag ctgacgccga gcgccagcgc
    13981 cactcgactg tccgacgacg acggcggcga ccaccccggg tgcatgccgg cgatggtttc
    14041 gtccgtgtcg agcgacaggc tgtttccgcc tggcaattcc acggcgacgt gcggtatctc
    14101 cgcgtccggt acaggcaggc ccagtaggcg gtagaacgcg atcgatcgtg ccagatcgtg
    14161 ggcgaagacc tggaagacgg ccgatgtcag tcgaatatcc atgccggcca cgctaggacg
    14221 gcgggcacgg cgctgtcgtg aacaaatcgg acacgcacgg tggtcgcttc gaataccctt
    14281 gctgagattt atgcctcggc agaatgagta gatctactcc ccctgcacaa gcgccctttt
    14341 gcactacgct tcgctacggc atacccaccg aatccgtttg cccgacacgg caacgccgaa
    14401 cacagactga catgagttaa ttcgtgaaac gcagtgcaat tactgggact gccgcgatcg
    14461 ccgcggcact cgcggtcctg ctgtcgggtt gcggctcgga caccaagacc gaacccagca
    14521 ccaccacgtc ggcgtcgaag tccacgtcga cgagcgccaa ggcggctccg tcgtggaagc
    14581 cgaaggtggc cccacgcgat gaggatgcca agggtccgca cccgacgatc gccagctaca
    14641 tccaggagaa caacatccag gaaaccccgg tcaagcgggg cgatcccggc tcacccacca
    14701 tcgacctgcc gatccccgac ggctgggcgc ccgggggtga ggacaccccc gaatgggcct
    14761 acggcgcaat cgtttacacc ggcccggacg ccggtgacta cacgccgagc atcgtcgccc
    14821 tggtgtcgaa gctgaccggg aacgtggacc cgcagcagat catcgacttg gcgcccggtg
    14881 agctcaacaa cctgccggga tggaaggcga tgaacgaggg agaggccagc acactcggcg
    14941 agtacccggc ctaccagctc ggtggcacgt gggttcagga cggtcagacg aagatcgtcg
    15001 cgcagaagac ggtcgtgatc cctggcagcg acggacttta cgtgctgcag ctcaacgccg
    15061 acggcctgga ggaccagaag gaaatcatcg gcgcggcaac agatgtgatc gattcgcaga
    15121 ccacgatcac gccctgatgt atcgatgatg gtcggacggt gttgtccaac aacatcttcc
    15181 gatctcatcg ctggtaacaa cagcgcgcta gatatcgctg gccgcttgga tcaccgcttc
    15241 gacggcatgg ccgatgtggt cggcagcgcg atcgggatcg ggttggccgg cgtcgagcca
    15301 cgcgatgacc gcctccaaag tgagggtcgg aagcagtcgc gaagcccact ggcgccactg
    15361 cccttcgttg attcggtcgg cgagctgtcg gttagcgatg tcgaccgcgg tggatcggat
    15421 ctcgtcgacg atgtcgcgga actctgcttc tcgcaccgcg tggcgaaaga ggaggcgaaa
    15481 cccgtctggg tcgcccgcgg cgccccgaag caaccgggag atgctgccct cgtcgaactc
    15541 gtcggtcccc acgatctcgg ataaccgcgc ggccgcacgc tcgaggacag cgcggtagag
    15601 gtcgctcttg gagtcgaagt ggcggtagag gatcacgtgg gtgacccccg cttctgcagc
    15661 gatgttgtcg aggctggtgc ctgcgaaccc cgaacgggca aaggcgcggg tggctgcgtc
    15721 gaggaggtgt tcgcgccgct cggcacgcgc cattcgacgg acggccgtct gagccattgc
    15781 acggaccccc tgccgggtct agtcttgttt aagtcatctt atacaagagt acttaaactt
    15841 gactggaggt cacgacggtg gccgtcacgc agttgccgct agagcagccc catccgctgc
    15901 gagtcccgcc gctgctgcgg gacatgcagg ccaatggctc agttcaccgt gttcgtaccg
    15961 aagtcggcga tccggcgtgg ctgatcaccg gacacgcgac ggtgcgccgc ctgctcgagg
    16021 acgaccgcct cgggcgatcc catccccagc ccgaggccgc ggcccgggcc ggggagtcgg
    16081 tgttgttcgg tggaccaacg ggcaatttcg ccaccgaacg cgccgaccac actcggatgc
    16141 gtaccctgct gcagccccat ttcacgccga aacacatgcg tgccttgacc ggacggatcg
    16201 aggcactgac cacgcaactg ctcgacacaa tggcagagca aggcccgccg gtcgacctgc
    16261 acaccgcggt cgcattgcca ttgccgatac tggtgatctg cgaattgctc ggcgtgccct
    16321 acgaggaccg cgaccaattc cgccgctgga ccaccgaggc ttcaaacaca cgtgaccgaa
    16381 ctctgtccgc gcacggtgtg tggcagttgt tcggctacgg gcagcggctc gtcgccgcca
    16441 agcgacacca acccggcgac gatgtgatct cgcgactgtg cgcaaccaaa ggggtacccg
    16501 acgacgagat cgcctggcta tcaatggcgt tactgttcgc cggacatgag acgacggtag
    16561 ttcagatcgg cctggccacg ctgctgctgt tagcgcagcg tgagcaatgg caggccctcg
    16621 tcaacgatcc cggcttggtg tcgcgggccg tcgaggagac cctgcgaagc tcgcgcaagg
    16681 gcggcggtgg gacgatcccg cgatatgcca gcgccgatct tgaagtcgac ggcgtcacca
    16741 tcaagaccgg agagctcgtc ctgatcgaca tcgcggccgc caaccaggat cctgtggcgt
    16801 tcgccgaccc cgatcgagtg gacatcacgc gttcgagcct ggcccatttg acgttcggct
    16861 acggcccacg gtattgcctg ggagcaccgc tcgcccgcgt cgagctgcag gtggccttgt
    16921 cccagttggc atctcggttc ccgacgctgc ggctggcggt cgaggtcgac gaattgacga
    16981 tgcgagccga cgtgctggtg ggcgggctca ccgagctgcc agtgcgctgg tagttcggcg
    17041 ttagttttct cggatgccgt cgagccgccg ggtggcgtcc tcgaagccgg ccacgagctc
    17101 ggcgatgatg tcggcgacag gccggatctc attcatccgg ccgacgatct ggccgaccgg
    17161 cattgcgacg gtgtcgggat tgtccgactc gttcatccgc tgatgggcct cgctgacgag
    17221 aatgttctgc agcggcatcg gtaacggctc aggcgcatcc ggagcatccc aggcgtcggt
    17281 ccagcgggac ttgagcaacc gtgccggctt cccggtgtag atgcgacggc gcacagtgtc
    17341 gctggagttc gcacgcagca gcgcttcctg gatcgtcgag acaccgctcg gcttgcggtg
    17401 gccgaggtcg tactccgccg acgtcaagaa cgccgagccc atccagaccc cttgcgcgcc
    17461 aagggccaac gccgcggcca cctgcctgcc ggtgccgata cccccggccg ccaacaccgg
    17521 cgccctaccc tcaagcgcat caacgatttc cggccacagc accatcgacc cgatctcgcc
    17581 ggtgtgcccg cctgcctcat ggccctgcgc gacgacgatg tcgacgccgt tctcgacgtg
    17641 gcgctgagcg tgtttggcac tgcccgcaag ggcggccacc gggacaccgg tctcgtgagc
    17701 ctggtcgatg acatccttcg gcggcgagcc caaagcgttg gcgatcagtt tgatcggatg
    17761 cttgagcgcc acctcgacgt gcgaccgcgc caccgaatgc agccagccga gcacgccctc
    17821 gttacgctcc ccctcgtcgg gcagcggcgg aacaccaagg tcggcaaggg ttttggcaac
    17881 gaagtcgcga tggctctgcg ggatcagctt gtcgatgtcg acggccgtgc cctcctgcgg
    17941 gaccttggcg ggcatgacga tgtcgacgcc gtacggcttg ccgtcggtgt tctcgtccat
    18001 ccagcacagg acgttctcga gatcttcggc ctcgttgaac cgcacgcacc cgagcacccc
    18061 gagcccgccg gccttgctga ccgcggctgc gaccttctcc gatggcgtga agacgaagat
    18121 cggatagtcg atgccgaagc ggtcacacag ctcggtccgc atcaggacga tgtccccaca
    18181 tggttggcgt gtgcatcgtc ggcgggccgc tcttcggttt gatctttggc ccagcgatag
    18241 tcgggctttc cggcaggtgt ccgatggatc tcgtcgaccc accaaacctt gcgcggcact
    18301 ttgtatcccg cgatctcctg acgcacgaac gtgtcgagtt cggcgagtgt cggattggtg
    18361 ccaggccggc ggtgcacgac ggcggccacg cagttgccgt accgctcgtc gggaacgccg
    18421 accaccaatg cgtcgaacac gtccggatga cccttgagtg cggcctccac ctcttcaggg
    18481 tagatcttct cgccgccgct gttgatcgac tgcgaaccac ggccgagcat cgtgacgctg
    18541 ccgtcctcat cgacctccgc gtagtctccc gggatcgcat agcggacacc gttgaaggtc
    18601 ttgaacgtct cggcggtctt cttctcgtcc ttgtagtagc cgactgggat gttgcccttc
    18661 ttggcgatca tcccgcgcac tccggagccc ggtttgacct cgttaccgtc ctcgtcgaga
    18721 acgacggtgc ggtggtcgat cgtcacccgc gggccgccag tgtgcgactg gcccttggcc
    18781 acgatgctgg ttccgccgaa gcctgtttcc gaggaaccga tcgagtcggt gatcacccgg
    18841 ttgggaagca gttcgaggaa cttctccttg atgctggtgg agaacaacgc ggcggtgctg
    18901 gcgagcagga acaggctgga caggtcgggc tcgtggccgc cgtcatgcag cttggtcaac
    18961 gcgtccagca gcgggcgtgc catcgcatcg ccggtgaaga acagcagatt caccttgtac
    19021 ttctcgatcg tggcccatac ctcgtcggca tcgaactccg gtgcgagaac agtggtttga
    19081 cccgagaaga gggacatcca ggtagccgac tgcgtcgcac cgtggatcat cggcgggatc
    19141 gggtagcgga tcatcggcgg attctccgca gcggcctttg ccaggtcgta ttcgtccttg
    19201 acgaactcgc ccgtcgcaaa gtcggtgccg ccgaacaaca ctcggtagat gtcctcgtga
    19261 cgccacat
//
LOCUS       DASRJA010000068        12766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4170578,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000068 DASRJA010000000
VERSION     DASRJA010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12766
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4170578"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VFC01_03040"
     CDS             <1..923
                     /locus_tag="VFC01_03040"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN78653.1"
                     /translation="LGIEVDPNFMNNGYIYASYIAADNYEVLSRFTVTDPSAAILTAD
                     PESEVVLLHGTQLAADDHLAGAVRFGPDGKLYWSVGNNDYYAVTTPPQGFTYPSNNAQ
                     DLSNIYGKILRLNPDGTVPADNPFVNTAGANPYIYAYGFRNPFRMTFTPDGQLLVGDV
                     GQEVWEEVDNVTAGGNYGWPLAEGPCNGIGTTSCLTPSPFANPIYAYLHTGGGNSITA
                     ILVYTGSTFGTSYQNKVFIADFNQQWIQELTCTPDYSSCASPTMFQAQAGHIVELIQG
                     PDGNIYQLTFAAPSLSPNSGELSRIAPSAG"
     gene            complement(1323..1937)
                     /locus_tag="VFC01_03045"
     CDS             complement(1323..1937)
                     /locus_tag="VFC01_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN78654.1"
                     /translation="MLLLLNVAHQSPSATAAALCPDVEVVFARGTGEQPGIGGVGQAF
                     VDSLRPQVGARSVGVYAVNYPAGDDYPNSAPSGAQDASARVQYMTANCPSTRIVLGGY
                     SQGAGVIDLITAPTAPAPGFTPAPMPPDVADHVVAVAVFGNPSMKSGGGPLTTISPLY
                     GAKAIDLCMPLDPVCSESLLPFMHYLYVQSGMVNQAASFVASRL"
     gene            2137..3777
                     /locus_tag="VFC01_03050"
     CDS             2137..3777
                     /locus_tag="VFC01_03050"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HZN78655.1"
                     /translation="MTSVSEHRAEAAAEHQIDIHTTAGKLADLRKRAEETLHPVGEAA
                     VEKTHARGKLTARERILALLDEDSFVELDALARHRSTNFGLADKRPLGDGVVTGYGTI
                     DGREVCIFSQDATVFGGSLGEVYGEKIVKVQELAIKTGRPLIGINDGAGARIQEGVVS
                     LGLYSKIFHNNILASGVIPQISLIMGAAAGGHVYSPALTDFVIMVDHTSQMFITGPDV
                     IKTVTGEDVTMEELGGAHTHMAKSGTAHYVASGEQDAFDYVRELLSYLPSNNYADPPR
                     YPAPVPTGAIEENLTDEDLELDTLIPDSPNQPYDMHEVITRILDDDEFLEVQAGYATN
                     IVVGFGRVDGRPVGIVANQPTQFAGCLDINASEKAARFVRTCDCFNIPIVMLVDVPGF
                     LPGTDQEYNGIIRRGAKLLYAYGEATVAKITVITRKAYGGAYCVMGSKDMGADVCVAW
                     PTAQIAVMGASGAVGFVYRQQLSEAAKKGEDVDALRLQLQQDYEDTLVNPYIAAERGY
                     VDAVIPPSHTRGYVGTALRLLERKITQIPPKKHGNIPL"
     gene            3774..4001
                     /locus_tag="VFC01_03055"
     CDS             3774..4001
                     /locus_tag="VFC01_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="HZN78656.1"
                     /translation="MSDEQHYEPHIKVIKGEPTEEELAAVIAVLADASARPAEPREQE
                     QNLWGHPVDRLRYPSFSWQRVTLLERTHMRR"
     gene            3998..4639
                     /locus_tag="VFC01_03060"
     CDS             3998..4639
                     /locus_tag="VFC01_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HZN78657.1"
                     /translation="MTRVVLASASPGRRKVLRQAGIDPLVILSGVDEDAVIARLDPDA
                     TPGTVTTALAAAKADAVAHALDPAVAADCVVIGCDSMLYRDSELLGKPASTDAAREGW
                     LTMAGKCGQLYTGHCVIRLRDNRITYRAADATATTVCFGVPLPADLDAYVSSGEPTAV
                     AGGFTLDGLSGWFIESIEGDPSAVIGIGLPTTRRLLERAGLTVAELWATNPVR"
     gene            4704..5183
                     /locus_tag="VFC01_03065"
     CDS             4704..5183
                     /locus_tag="VFC01_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78658.1"
                     /translation="MADFINETIMLGTCRVGVAQPRRGDQCLRKALTYSDERLQVLTL
                     VTPDGAYGGPAQVAFNQASEIRAQGYSLTIAGGCMGYRTTPETLYGTSVRLCRCPASR
                     AIHGARGHRRTVGDLLILKNRRNIDAVHVHLGRDLVSMPAALLGLLLKKRVILQPTE"
     gene            5451..6662
                     /locus_tag="VFC01_03070"
     CDS             5451..6662
                     /locus_tag="VFC01_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN78659.1"
                     /translation="MRLVVHDYSGHPGQVHLSRELARRGHQVEHQFCASYTTGRGATR
                     RRKGDPDTFTVRAVELPRPFARYSPILRVWQELQYGQIAARAVLAARPDLVVLSNVTL
                     ISLFLLTLCLRIRRVPYVFWQQDIYSDAIGVVARKRLGRVGGLIGRLASRTECSVARG
                     AAAIVAISDTFIEQLDAWGIPASKVQVIPNWAAIDEMPARPRDNAWAKAHGLVGSPVV
                     MYAGTLGLKHNPSVLATLARSAPTGCRVVVVSEGLGREWLESNAASEPRLVLLDYQPY
                     EQLPDMLASADVLVVVLEQDASRYSVPSKVLNYLCAGRPVLALLPANNAVAQMIEAAQ
                     AGLVVDPGNADEASAVLNQLLTDSNIRQKMGASARRYAEQAFDVEAVGDLFDSVFRAV
                     YDRRAHSQKQT"
     gene            6755..7753
                     /locus_tag="VFC01_03075"
     CDS             6755..7753
                     /locus_tag="VFC01_03075"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN78660.1"
                     /translation="MISAQETVLVAGAGGFIGGHLARTLSEQGLRVLGADVKPLDEWY
                     QIPSGVDARQLDLARLDDCHEAVRGVDVVYNLAADMGGMGFIETHKADCMLSVLISTH
                     MLLAAKDAGVQRFFYSSSACVYAADKQVDANVTALKEDDAYPAMPEDGYGWEKLFSER
                     MSRHFLEDFGLQTRVARYHNVYGPIGSFEGGREKAPAALCRKVAQAVLSGQHEIEIWG
                     DGEQTRSFMYVDDCVDGTLKVTAGHSPLPVNVGSSELVSINEMVSVIEQIAGITVKRN
                     YKLDAPLGVRGRNSDNTLIQEIYGWEPSIRLADGLERTYQWIFDILSARERRSAVG"
     gene            8155..9042
                     /locus_tag="VFC01_03080"
     CDS             8155..9042
                     /locus_tag="VFC01_03080"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HZN78661.1"
                     /translation="MTLPADPDPPLQSYAHPERLVTADWLSGNLGRPGLAIVESDEDV
                     LLYDTGHIPGAVKIDWHTDLNDPHVRDYINGKQFAELMDRKGISRDDTVVIYGDKCNW
                     WAAYALWVFTLFGHPDVRLLDGGRDLWINDGRDTTLDVPSKETTGYPVVDRDDTPIRA
                     FKDDVLDILGKQPLIDVRSPQEYTGERTHMPDYPEEGALRGGHIPTARSIPWAKAAQD
                     NGRFRGRDELEQLYGFLAPDDKTVVYCRIGERSSHTWFVLTHLLGKQGVRNYDGSWTE
                     WGNAVRVPVAVGEEPGEAP"
     gene            9039..9455
                     /locus_tag="VFC01_03085"
     CDS             9039..9455
                     /locus_tag="VFC01_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HZN78662.1"
                     /translation="MTMPAALTEVVSDFKEVQGQDKLRLLLEFADELPPLPTELEEAA
                     MEPVPECQSPLFLHVDAADREKVRLYFSAPAEAPTTRGFAAILATGLDQQPAADILAV
                     PDDFYTDLGLAALISPLRLRGMSAMLARIKRRLRGE"
     gene            9572..11371
                     /locus_tag="VFC01_03090"
     CDS             9572..11371
                     /locus_tag="VFC01_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HZN78663.1"
                     /translation="MASHASSRISKVLVANRGEIAVRVIRAAKDAGLPSVAVYAEPDA
                     DAPHVRLADEAFALGGQTSAESYLDFAKLLDAAEKSGANAIHPGYGFLSENADFAQAV
                     IDAGLIWIGPSPQSIRDLGDKVTARHIAARAKAPLVPGTPDPVKDADEVIAFANEYGL
                     PIAIKAAFGGGGRGMKVARTVEEIPELFDSATREATAAFGRGECFVERYLDKPRHVEA
                     QVIADQHGNVVVAGTRDCSLQRRYQKLVEEAPAPFLTDAQRKEIHESAKRICKEAHYY
                     GAGTVEYLVGQDGLISFLEVNTRLQVEHPVTEETAGIDLVLQQFRIANGEKLDITEDP
                     EPRGHAIEFRINGEDAGRGFLPAPGPVSKFEAPSGPGVRMDAGVETGSVIGGQFDSML
                     AKLIVHGGTREEALARARRALDEFHVEGLATVIPFHRAIVSDPAFIGDGDGFTVHTRW
                     IETEWNNTVEPFTGGDPIEEEDTIPRQTVVVEVGGRRLEVSLPGDLAIGNGGAPAGGG
                     NVLRKKPKPRKRGAHAGAAASGDAVTAPMQGTVVKVAVEEGQQVSAGDLVVVLEAMKM
                     ENPVTAHKDGTITGLAVEAGAAITQGTVLAEIK"
     gene            complement(11456..12151)
                     /locus_tag="VFC01_03095"
     CDS             complement(11456..12151)
                     /locus_tag="VFC01_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZN78664.1"
                     /translation="MKITVVGGSGLIGTKVVDLLTADGHDVVAASRASGVDVLTGDGL
                     AEALSGADALIDVLNSPSFEDDPVMEFFTKATTNLVDEAKRAGVGHYVALSIVGVDGL
                     PESGYMRAKVVQEKIIAESGLPYTIVRATQFAEFTDAITDTMFAGDEVRVPDALIQPV
                     AADDVARDVARAAVADPLNGIVNIGGPEKVSFERMARDVLGRRGTDTTVVVDPQARYF
                     GTLLEQRSLVTPD"
     gene            12168..12479
                     /locus_tag="VFC01_03100"
     CDS             12168..12479
                     /locus_tag="VFC01_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78665.1"
                     /translation="MEPVEINAGAWYLRALRADDRIDDRPALAELGETDGGYVQNRAA
                     QWESDTTYSWAVCEPTTGELLAEVTLYPATGLIGLRARPGHADAAQAAGHAVRRFADS
                     L"
     gene            complement(12484..>12766)
                     /locus_tag="VFC01_03105"
     CDS             complement(12484..>12766)
                     /locus_tag="VFC01_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZN78666.1"
                     /translation="AAYAVLHTVDTPDVGSDTVRRVLDAARSSASGLARARYGDRPGH
                     PVVIARRYWPDLLDGLRGDEGARAFLAGRPDVVAVDCADLASGADIDTR"
BASE COUNT         2378 a         4171 c         4050 g         2167 t
ORIGIN      
        1 tactcggtat cgaggtggat ccgaacttca tgaataatgg ctatatttac gcgagctata
       61 tcgcggcgga caactacgag gtgttgtcgc gcttcaccgt aactgaccca tcggctgcga
      121 ttctgaccgc cgaccccgag tccgaagtcg tcctgctgca cggtacccag ctggctgccg
      181 acgatcatct cgccggtgcc gtgcgtttcg gcccggatgg caagctgtat tggtcggtgg
      241 gcaacaacga ttattatgcc gttacgacgc ccccgcaggg cttcacttac cccagcaaca
      301 acgcccagga tctttccaac atctacggca agattttgcg gctaaatcct gatggcacgg
      361 tgcccgccga caacccgttc gtcaacacgg ctggcgctaa cccgtacatc tacgcctacg
      421 gtttccgcaa cccattccgg atgacgttta cacccgacgg ccaactgcta gttggtgatg
      481 tcggccagga agtctgggaa gaggtcgaca acgtgaccgc tggcggcaac tacggctggc
      541 cgctagccga aggcccctgc aacggcatcg gcaccaccag ctgtttaacg ccttccccat
      601 tcgccaaccc gatttatgcc tacctccata ccggcggcgg caattcgatc acggcaatct
      661 tggtctatac cggctcgaca ttcgggacaa gctatcaaaa caaggtgttc atcgcggact
      721 tcaaccagca atggatccag gagctgacct gcactcctga ttactcgtcg tgcgctagcc
      781 cgacaatgtt tcaggcccaa gccggccaca tcgtcgaact gatccaggga ccggatggca
      841 acatctatca actgacattc gctgctccgt ccctttcgcc caattccggt gagctttcac
      901 gcattgcccc atcagccggg taagcaagca ccgatcgcca tcgtcaccgc cacatcaacc
      961 attggcttgg cactctgggc cccgaccttt catcgcaagg ttcgagcgac ccaaatccgg
     1021 ctccaccgcg ggccttgcat gggcgtgttt ggccacggca tcacgttggg ggctgctgac
     1081 tacggtgcag tctgcacgac ccgccaaccc cactgccgca agggaattcg gcggttcacg
     1141 ccgaaaccgg aatgccatca aacagattcg gccgctatcg cattgaatca aaaccgtaag
     1201 tgagcggaat ctatgtccga gcaacaacac gccgacgatg acgaggtata cccgtgccgc
     1261 cgagggccgt cccttcgagg cgtgaaatgc gcagcggccg acttgcgctc gctcgcacct
     1321 ggctaaaggc gactcgcgac gaaactcgcg gcctgattca ccatcccgga ctgcacatag
     1381 agatagtgca tgaaggggag caagctctcc gagcaaaccg gatccaaagg catacacaag
     1441 tcaatggcct tggccccgta cagcgggctg atcgtggtca gcggcccacc cccggatttc
     1501 atcgatggat ttccgaaaac ggcgaccgcg acaacgtgat cagccacatc aggcggcatc
     1561 ggagctggag taaaccccgg agcgggtgct gtaggggcgg tgatgagatc gatcacgccc
     1621 gcgccttgag aatatccacc gagcacaatc ctggtgctgg gacagttcgc ggtcatgtac
     1681 tggacacgag cgctcgcatc ctgtgcacca gagggggcgg agttgggata atcatcgccg
     1741 gcagggtaat tgactgcgta caccccgacc gacctagcgc caacttgcgg gcgcaacgag
     1801 tcgacaaatg cctgtccaac tcctccaata ccaggttgct cgccggtgcc gcgtgcaaac
     1861 accacctcga cgtccgggca tagcgcagca gcagtagcgg aagggctttg gtgggcgaca
     1921 ttgagcaata acaagatcgt caatgccgca gcaccctgga tatgagcaaa ctggcctccg
     1981 gtcacttgtc aatgctggca tacgcagcgg acctcggcga ctcgcgcgca ctatgtcggg
     2041 gccgtgttgc cagctccaga tgaacgtcgg caaggatcgg ccgcagtgtt gggcgggccc
     2101 gacgacctga cggccttgcc gatagcatcg acatccatga cgagcgtttc tgagcacagg
     2161 gccgaggcgg ccgccgagca ccagatcgat atccacacca ccgcgggcaa gctggccgac
     2221 ctccgcaagc gggccgaaga gaccctgcac ccagttggcg aggccgcggt cgagaagacg
     2281 cacgcgaggg gcaagctgac tgcccgtgag cgcatcctgg cgctgcttga cgaggattcc
     2341 ttcgtcgagc tcgatgcgct ggcccgccac cgcagcacca acttcggcct ggccgacaag
     2401 cggcccctcg gtgacggtgt cgtgaccgga tacggcacca tcgacggccg cgaggtgtgc
     2461 atcttcagcc aggacgcgac ggtattcggc ggcagcctcg gcgaggtcta cggcgaaaag
     2521 atcgtcaagg tccaggaact tgcgatcaag accggccgtc cgctgatcgg catcaacgac
     2581 ggcgccggcg cgcgcatcca ggagggcgtc gtctcgctgg gcttgtacag caagatcttc
     2641 cacaacaaca tcctggcgtc cggcgtgatc ccgcagatct ccttgatcat gggcgccgcg
     2701 gcgggcggcc acgtgtactc ccccgcgctc accgacttcg tcatcatggt cgaccacacc
     2761 agccagatgt tcatcaccgg gcccgacgtg atcaagaccg tcaccggcga ggacgtcacc
     2821 atggaggaac tcggcggcgc acacacgcac atggccaagt ccggcactgc gcactacgtc
     2881 gcctcgggcg agcaggacgc cttcgactac gtccgcgagc tgctgagcta cctgccttcc
     2941 aacaactacg ccgatccacc gcgctatccc gcgccggtgc cgacgggtgc gatcgaggag
     3001 aacctgaccg acgaggacct cgagctggac acgctgatcc ccgattcacc gaaccagccg
     3061 tacgacatgc acgaggtgat cacccgcatc ctcgacgacg acgaattcct cgaggtgcag
     3121 gccggctacg ccaccaacat cgtggtcggg ttcggccgcg tcgacggccg accggtcggc
     3181 atcgtcgcca accagccgac ccagttcgcc ggctgtctcg acatcaacgc ctcggagaag
     3241 gccgcccggt tcgtgcggac ctgcgattgc ttcaacattc cgatcgtcat gctggtggac
     3301 gtgccgggct tcctgccggg caccgaccag gagtacaacg gcatcatccg ccgcggggcc
     3361 aagctgctct acgcgtatgg cgaggccacc gtcgccaaga tcaccgtgat cacccgcaag
     3421 gcctacggcg gcgcctactg cgtgatgggg tccaaggaca tgggcgcaga cgtctgcgtc
     3481 gcatggccga cggcgcaaat cgcggtgatg ggcgcctccg gggcggtcgg cttcgtctac
     3541 cgccagcagc tgtccgaggc cgccaagaag ggcgaggacg tcgatgcgct gcgcctacag
     3601 ctgcagcagg actacgagga caccctcgtc aacccgtaca tcgccgccga gcgcggctat
     3661 gtcgacgcgg tgatcccgcc gtcgcacacg cgcggttatg tgggcaccgc gctgcgtctg
     3721 ctggagcgca agatcactca gattccgccg aagaagcacg ggaacattcc actgtgagcg
     3781 acgaacagca ctacgagccg cacatcaagg tcatcaaggg cgagccgacc gaggaggagc
     3841 tcgccgcggt gatagccgtg ctcgctgacg cgtcggcgcg tcccgccgag cccagggagc
     3901 aggagcagaa cctgtggggc catccggtgg atcggctgcg ctatcccagc ttcagctggc
     3961 agcgggtgac cctgctggaa cggacgcaca tgcgccgatg acgcgggtgg tgcttgcgtc
     4021 ggcctctccc ggtcgtcgta aggtgttgcg ccaggcggga attgaccccc tcgtcatcct
     4081 ctccggggta gacgaggacg ccgtcatcgc gaggcttgac cctgacgcca cgccaggcac
     4141 tgtaactacg gcgttggccg cagcgaaggc cgacgctgtc gcgcacgccc tggatcctgc
     4201 tgtcgccgcc gattgcgttg tgatcggctg tgattcgatg ctgtaccgag acagcgagct
     4261 gttgggcaag ccggcgtcga ccgacgcggc gcgcgaaggc tggctgacga tggccggcaa
     4321 gtgcggacag ctgtacaccg gacattgtgt gattcgcttg cgcgacaaca ggatcaccta
     4381 tcgcgccgct gacgcgactg caaccacggt ttgtttcggc gtacccttgc ctgccgactt
     4441 ggacgcttat gtgtcgagcg gcgaacctac ggcggtggcc ggcggcttca cgctcgacgg
     4501 actcagcgga tggttcatcg agagcatcga aggagatccc tcggcagtta tcggtatcgg
     4561 cctaccgacc acacgacggt tgttggagcg ggccggcctg acagttgcag agctctgggc
     4621 gaccaatcca gtccgatgag cgcgacaaga cgccggattg ccgttacgta tgtcggcgag
     4681 cgccgcaaag agtcgagaca tcgatggcag acttcatcaa cgagaccatc atgctgggta
     4741 cctgccgggt cggcgtggct cagccacgcc gaggagacca atgcctcagg aaggctctga
     4801 cgtatagcga tgaacgtctt caggtgctga cgctcgtgac tccggatggc gcctacggtg
     4861 gtcctgcgca agttgcgttc aatcaggcga gtgagattcg tgcccagggt tactcattga
     4921 cgatcgcggg cggctgcatg ggttaccgca cgacgcccga gaccctgtac ggcacgtcgg
     4981 tgcgactctg caggtgcccg gcaagtcgtg ccattcacgg ggcacgcggg catcgccgca
     5041 ccgtcggtga tctattgatt ctcaagaatc gccgaaacat cgacgcggtg cacgtgcact
     5101 tgggcaggga tctggtttcg atgccagcag cgctactcgg attgcttctc aagaaacgcg
     5161 tgatccttca acccacggaa tgatcaccaa atccaagcat ccgatcgcgc gggcgctcga
     5221 tcgcttcctg acccggccga tcttgcggcg tgccgtccgg gttaccgccg tggcgacttc
     5281 tgcttcgccc cagcgtcttt cacccgcgtg ccacggccca gcacgcggtc gtctacgcag
     5341 gacatctgac catctttgag gtctggacgc acgcggccgc ccgcttgctg gcatgctagc
     5401 cgggtgacct tgcgcctccc gcttcgcgaa tccattgggt gctctttgag atgaggctgg
     5461 ttgtccacga ctattccgga caccccggac aggtccacct cagccgcgag ctcgcccgcc
     5521 gaggacatca ggtagagcac cagttctgcg cgtcatatac gaccggacgt ggagcgacgc
     5581 ggcgccgcaa aggcgatcca gacaccttta ctgtgcgtgc cgtcgagcta ccgaggccgt
     5641 tcgctcgtta ctcacccatc ttgcgagtgt ggcaggagct gcagtacggc cagatcgctg
     5701 ctcgggccgt cctggccgcg cgaccggatc tcgttgtgct gtccaacgta accttgatat
     5761 ccctgtttct gctcacattg tgccttcgga tacggcgggt gccgtatgtg ttttggcagc
     5821 aagacattta cagcgatgcg ataggcgtgg ttgctcgtaa gaggctgggt cgggttggcg
     5881 gactcatcgg caggcttgcg agccgcaccg aatgcagtgt cgcgcgaggc gccgccgcaa
     5941 ttgtcgcgat cagcgacaca ttcatcgagc agctcgacgc gtggggcatc ccagcgagca
     6001 aagttcaggt cattcccaac tgggccgcga tcgacgagat gcccgcccgg ccccgggaca
     6061 acgcctgggc gaaggctcat gggttggttg gttctcccgt tgtgatgtac gcgggcacgc
     6121 tcgggttgaa acacaatccc tcggtgctcg cgacactcgc gcggtcagcg ccgaccggct
     6181 gccgcgtcgt ggtcgtatcc gaggggctgg gccgcgaatg gctggaatcg aacgccgcgt
     6241 cagagccgag gctggtgctg cttgactacc agccctatga gcagctcccc gacatgctcg
     6301 cgagtgccga tgtgctggtg gtcgtgctgg agcaagatgc gagccgctat tcggttccgt
     6361 cgaaggtgtt gaactacctt tgcgccggcc gtccggtctt ggcgctgctg cctgccaaca
     6421 acgccgtcgc tcagatgatt gaggctgcgc aggcggggct cgtggtagat cccggcaacg
     6481 ccgatgaggc gtctgccgtg ctcaaccaac tgctcacgga ttcgaatatc cggcagaaga
     6541 tgggagcatc cgctcgccgc tacgccgaac aggccttcga cgtagaggcc gtcggtgacc
     6601 tctttgattc ggtattccgt gccgtttacg atcgacgcgc tcattctcaa aagcaaacgt
     6661 agattactct cgacagcaaa cgtaggttcc cgtatgccaa gattttacga tcgtcaagca
     6721 ttagaggcgt accacgccct aggatggact gcaggtgatt agcgcgcaag agacggttct
     6781 ggtcgcaggg gcgggtgggt tcatcggcgg acatctcgcc aggaccctta gcgagcaggg
     6841 cctgcgcgtc cttggtgccg atgtcaagcc gttggacgag tggtaccaga tcccaagcgg
     6901 cgtggatgct cgccagctgg acctcgccag gctcgacgat tgtcacgaag ccgttcgtgg
     6961 cgtcgacgtc gtgtacaacc ttgcggccga catgggcggc atggggttca tcgagaccca
     7021 caaggccgac tgcatgctgt cggtgctcat cagcacccac atgctgctgg ccgcgaaaga
     7081 cgccggggtc cagcgattct tctactccag ttctgcctgc gtatacgccg ctgacaagca
     7141 ggtggacgcc aacgtcaccg cactcaagga ggacgacgcc tacccggcga tgccggaaga
     7201 cggttatggg tgggagaaac tgttcagcga gcgcatgagc cggcacttcc tggaggactt
     7261 cggcctgcag acgcgggttg cccgctatca caacgtctac ggccccatcg gcagctttga
     7321 gggtggccgc gaaaaggcgc ccgccgccct ctgccgcaaa gtcgcacagg ctgtgctctc
     7381 cggccagcac gagatcgaga tctgggggga cggcgagcaa acccgtagct tcatgtatgt
     7441 cgacgactgc gtcgacggaa cgctgaaagt gaccgccggg cacagccccc tgcctgtcaa
     7501 tgtcggcagt tcagaactgg tgagcatcaa cgagatggtc agcgtcatcg agcaaatcgc
     7561 cggtatcacg gtaaagcgga actacaaatt ggacgcgcct ctcggcgtgc gcggacgcaa
     7621 tagcgacaac acgttgattc aggaaattta cggttgggaa ccgtcgatcc ggctcgctga
     7681 cggcctcgag cgcacgtacc agtggatctt cgacatactg tcagcgcggg aacgccggtc
     7741 agccgtgggc tgaccggtca gccgtcggct atcaggcgcc gagcgcggtg cctgcaggat
     7801 cggccaccac ttcgagccaa attgacgacg gatacgcacc ctcggcgttt gcgagcgttt
     7861 gcggaggtaa cgccgcgctc ggtttcatcc gattgctgta ggtcggaatg ctctcctaca
     7921 cccgctgttg cgcggcatcg tgcaacagcg caggcgagcc gggtctcaaa ccgcggattg
     7981 tccgcgagat aagcatgaag cagggcgcgc tcaggccaga agaacgcggc ggccttggtc
     8041 atcgccgaac ttgggtccgt tgtcgggaaa tcgctgaagg caaagttaag ccaatcggct
     8101 cggcagccat atgccaacca cggcgcaatt cagagatctc gctaggctca tggtgtgacg
     8161 ctgcctgccg atcccgatcc ccccctgcag tcgtatgccc atcccgaacg cctggtcacc
     8221 gccgattggc tatcgggcaa cctgggccgc ccggggcttg ccatcgtcga gtccgacgaa
     8281 gacgtgctgc tctacgacac cggccacatt cccggcgcgg tgaagatcga ctggcacacc
     8341 gacctcaacg acccgcacgt ccgcgactac atcaacggca agcagttcgc cgagctgatg
     8401 gaccgcaagg gcatctcccg cgacgacacc gtggtgatat acggcgacaa gtgcaactgg
     8461 tgggcggcct acgcactgtg ggtgtttacc ttgttcggcc atcccgatgt ccggctgctc
     8521 gacggcgggc gtgacctctg gatcaacgac ggtcgcgata ccacgctgga cgtgccgtcc
     8581 aaggagacca ccggctaccc cgtcgtcgac cgcgacgaca cgccgatccg cgccttcaag
     8641 gacgacgtcc tggacatcct tggcaagcaa ccgctgatcg atgtgcggtc gccgcaggag
     8701 tacaccggcg agcgcaccca catgccggac tatcccgagg aaggcgcgtt gcgcggcggc
     8761 cacatcccca ccgcccggtc cattccgtgg gcaaaagccg cgcaggacaa cgggcgcttc
     8821 cgcggccgcg acgaactcga acagctctac ggcttcctcg ctcccgacga caagaccgtc
     8881 gtgtattgcc gcatcggtga gcggtccagc catacctggt tcgtgctgac ccacctgctg
     8941 ggcaagcagg gcgtgcgcaa ctacgacggc tcctggaccg aatggggcaa cgccgtgcgg
     9001 gtgcccgtcg ccgtcggaga ggaacccggc gaagcgccat gaccatgccg gcggctttga
     9061 ccgaggtggt gtcggacttc aaagaggtgc aggggcagga caagctgcgg ctgctgctgg
     9121 agttcgccga tgagctgcca ccgctgccta cggagctcga agaggctgct atggagccgg
     9181 tgcctgaatg ccagtcgccg ctgtttctgc acgtcgacgc ggccgaccgg gagaaagtca
     9241 ggctgtactt cagcgcgccc gccgaggcgc cgacaacccg gggattcgct gccattctcg
     9301 cgacaggcct cgaccagcaa cctgccgccg acatcctggc tgtgcccgac gacttctaca
     9361 ctgatctggg tttggcggca ttgatcagcc cgctgcgatt acgaggcatg tcggccatgc
     9421 tggcgcggat caagcggcgg ctccggggcg agtagaaaag ctactcatcg gtaggagacc
     9481 accgctcggc gcgctcacat gagcgcccct taaactgccg gggatacatt cttaaagacg
     9541 tttcttagag aacatgccgt caggaggcgc agtggctagt catgccagct caaggatctc
     9601 caaggtgctg gtcgccaatc gcggggagat cgcggtccgg gtgatccgag ctgccaagga
     9661 tgccgggctt ccaagcgtgg ccgtctacgc cgaaccggac gccgatgcgc ctcacgtacg
     9721 gctggccgac gaggccttcg cgctcggcgg ccagacctcg gccgagtcct acctcgactt
     9781 cgccaagctg ctcgatgcgg ccgagaagtc gggcgcgaac gcgatccacc cgggctacgg
     9841 attcctttcc gagaacgcgg atttcgcgca ggcggtgatc gacgcgggcc tgatctggat
     9901 cggccccagc ccacagtcga tccgcgacct cggcgacaag gtgaccgcgc gccacatcgc
     9961 tgcgcgcgcc aaggcgccgc tggtgcccgg caccccggac ccggtgaagg acgccgacga
    10021 ggtaatcgcg ttcgccaacg agtacggcct gccgatcgcg atcaaggcgg cgttcggcgg
    10081 cggcggccga gggatgaagg tcgcccgcac cgtcgaggag atccccgagc tgttcgactc
    10141 ggccacgcgc gaggcgaccg cggcgttcgg ccgcggcgag tgcttcgtcg agcgctacct
    10201 ggacaagccg cgccacgtcg aggcgcaggt gatcgccgac cagcacggca acgtcgtcgt
    10261 cgcaggcacc cgcgactgct cgctgcagcg ccggtaccag aaactggtcg aggaggcgcc
    10321 cgcgccgttt ttgaccgacg cgcagcgcaa ggagatccac gagtcggcca agcgcatctg
    10381 caaggaggcg cactactacg gcgcaggcac cgtggaatac ctggtgggcc aggacgggct
    10441 gatctccttc ctcgaggtca acacccgcct gcaggtcgag caccctgtca ccgaggaaac
    10501 cgccggcatc gacctcgtgt tgcagcaatt ccggattgcc aacggcgaga agctggacat
    10561 caccgaggat ccggagccgc gcggccacgc gatcgagttc cgcatcaatg gcgaggacgc
    10621 cggccgcggc ttcctgccag cgcccggccc ggtcagcaag ttcgaggcgc cgagcggccc
    10681 cggcgtacgg atggacgctg gggtcgaaac cggctcggtg atcggcggac agttcgactc
    10741 aatgctggcc aagctgatcg tgcatggcgg cacccgtgag gaggcgctgg cccgcgcccg
    10801 ccgcgcgctg gacgagttcc acgtcgaggg cctggccacc gtgatcccgt tccaccgcgc
    10861 gatcgtgtcc gatcccgcgt tcatcggtga cggcgacggc ttcaccgtgc acacccgctg
    10921 gatcgagacg gagtggaaca acaccgtcga gccgttcacc ggcggggacc cgatcgagga
    10981 agaggacacc atcccgcggc agacggtcgt ggtcgaggtc ggcggacgtc ggctcgaggt
    11041 gtcgctgccc ggcgacctcg cgatcggcaa cggcggcgcc cccgccggcg gcggcaacgt
    11101 ccttcgcaag aagcccaagc cccgtaagcg cggcgcgcac gccggtgccg ccgcatccgg
    11161 cgacgcggtg actgccccga tgcagggcac cgtggtcaag gtcgccgtgg aagagggcca
    11221 gcaggtgtcg gcgggcgatc tcgtcgtcgt cctcgaggcg atgaagatgg aaaatccggt
    11281 caccgcccac aaggacggca ccatcactgg gctcgccgtc gaagccggcg ccgcgatcac
    11341 ccagggcacg gtgctggcgg agatcaagta gctctcgcgc gccgaacgtt ccttaccgct
    11401 cgaaattttc gcgatttttc gagtggtacg gaacgttcgc gcccgtccgg gcagcctagt
    11461 cgggggttac caggctgcgc tgttccagca gcgtgccgaa gtagcgggcc tgcggatcga
    11521 cgacgaccgt cgtgtcggtg ccgcgcctgc ccaacacgtc tcgagccatc cgttcgaacg
    11581 aaaccttttc cggcccaccg atattcacga tgccgttgag tgggtctgcg acggccgcac
    11641 gggcgacgtc cctcgcgacg tcgtcggcgg cgaccggctg aatcaacgcg tcgggaaccc
    11701 gcacctcgtc gccggcgaac atcgtgtcgg tgatggcgtc ggtgaactcg gcgaactgcg
    11761 ttgcccgcac gatcgtgtat ggcaggccgg actcggcgat gatcttttcc tgcaccacct
    11821 tcgcccgcat gtaaccgctt tcgggtaacc cgtcgacgcc gacgatcgac agcgcgacgt
    11881 agtggccgac gccggcgcgc ttcgcctcat ccaccagatt ggtggtcgcc ttcgtgaaga
    11941 actccatcac gggatcgtct tcgaacgacg gggaattcag aacgtcgatg agcgcgtcgg
    12001 cgccactcag cgcctcggcc aggccgtcgc cggtcagcac gtcgactccc gaggcgcgtg
    12061 aagccgcgac gacgtcgtgc ccgtcggcgg tcagcaggtc gaccaccttc gtgccgatga
    12121 ggccgctccc gccaacaact gtgatcttca tgcctaccat cagacgcgtg gaacccgtcg
    12181 aaatcaatgc cggtgcttgg tatttgcgcg ccctgcgcgc agatgaccgc atcgacgacc
    12241 ggccggcgct cgccgaactg ggcgaaaccg acgggggtta cgtacagaac cgagctgccc
    12301 agtgggagtc cgatacgacg tactcctggg cagtgtgtga gccgacgacg ggtgaattac
    12361 tcgccgaggt gacgctgtat ccggccaccg ggctgatcgg cttgcgcgca cgacccggcc
    12421 acgccgacgc cgcacaggcc gctgggcacg ccgtgcggcg gttcgccgac agcctgtagg
    12481 cagctatcgc gtgtcgatgt ccgcgcccga cgctaggtcg gcgcagtcga cggcgaccac
    12541 gtcggggcgg ccagcgagaa atgcccgcgc gccctcgtcg ccgcgtagcc cgtcgagcag
    12601 atccggccag tatcggcgcg cgatgacgac gggatgcccc ggccggtcgc cgtaacgggc
    12661 gcgggcaagt cccgacgccg acgagcgcgc cgcatcgagc acgcgtcgca ccgtgtcgga
    12721 gccgacgtcg ggcgtgtcga ccgtgtgcag tacggcgtag gcggcg
//
LOCUS       DASRJA010000069        15379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3325278,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000069 DASRJA010000000
VERSION     DASRJA010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15379
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3325278"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFC01_03110"
     CDS             <1..620
                     /locus_tag="VFC01_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN78667.1"
                     /translation="IGAVFNNAGVATASSVLDAIPEDDQWLWNINFHGVVNGTRAFLP
                     ILVEQNEGAIVNTSSVFGLVGMPHQSAYCSAKFAVRGFTDSLRQELRGTGVTAINVHP
                     GGINTNIVRNARWRKDPHGRGRSQEQMVQEFAAITLTQPDKAAEIIHRGVEQGKARIL
                     VGPDAYLFDTLGRLAPTRYYDILGRLESVLRRRSQTNVVPEVAVP"
     gene            617..1960
                     /locus_tag="VFC01_03115"
     CDS             617..1960
                     /locus_tag="VFC01_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN78668.1"
                     /translation="MTAAVHLPPGPRWPVPLQGLAYIASRRRMMQALRKRYGSSFTVR
                     LPFFGPTVVISDPALVKQFFQTRTDVVRNVEPNLDLVLGPGSTFGLQDEQHRRRRKLL
                     VPPLHGKRMRAYEDLVEEETLKEIATWPEGREFAVLPSTNRITLNVILRAVFGAEGAE
                     FDALRKVMPPLVELGSHLAFLPWLHRDLGPWSPWGRFLGQRRQFDEIVGSLIDRVDAD
                     PNIEQRDDVLSLMLQARYEDGTAMSRSDLADELFTLLAAGHETTATQLAWAVERLRRH
                     PAVLSRLVDEVDTGSSDLLQATIHEVQRNRPVIDGAARQVIAPTMPLGEWVIPHGYTV
                     QVDIPLTHQNAYPDPERFDPDRFMESSPDMYSWVPFGGGARRCLGAAFANMEMNVVLR
                     TLLRKFRFVPTASRPERLHSRGVAFAPSGGGRAAVYRRIHTAASDNLRKSARVSV"
     gene            1965..2969
                     /locus_tag="VFC01_03120"
     CDS             1965..2969
                     /locus_tag="VFC01_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN78669.1"
                     /translation="MRAVVITKHGDLSVLQVQERPDPPPPGPGQVCITVRAAGVNFAD
                     HLARVGLYPDAPKPPMVVGYEVAGVIEAVGDGVDAARVGERVFAGTRFGGYAEIVNVQ
                     ALDAVPIPDSLTFERGAAIPVNYATAWAALHGYGSLRPGERVLIHAAAGGVGIAAIQL
                     AKAAGAEVHGTASPGKHARLAELGVDRAIDYRRDGWWKGLPPYDIVLDAIGGKSLKRS
                     YDLLRPGGRLVGYGASAMQQGEKRSLRTALPQLLSMVRGFNLVKQLSESKTLIGLNML
                     ALWDDRGTLQPWIEPLSKAMSDGVVQPVVHAAVPFANAGEAHRILAARENVGKVVLVP
                     "
     gene            complement(2966..3745)
                     /locus_tag="VFC01_03125"
     CDS             complement(2966..3745)
                     /locus_tag="VFC01_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN78670.1"
                     /translation="MKVHHLNCGTMRMPTAPMVCHVLLIESNNGLVLVDSGFGTHDCD
                     DPRRVGPTRRMVRAILAHEETVAHQIKRLGFVRDDVRHIVITHMDMDHAGGLSDFPHA
                     QIHLTAAEALGAIWAPSRQEKIRYRSAQWAHEPNIVEHDPRGEKWRGFAAAKELDEIS
                     PGIALVSLPGHTRGHACVAVDAGHRWVLHAGDAFYHYGTLDGTAVPRALTAMESMVAF
                     DRKAVLANHARLVELYRRQDPDLMIVCAHDPTLLERAQATA"
     gene            complement(3742..4362)
                     /locus_tag="VFC01_03130"
     CDS             complement(3742..4362)
                     /locus_tag="VFC01_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78671.1"
                     /translation="MADSSLPRTQEHRRIEAERRLVQAAAELVGEVGPGKVTLANVGE
                     RAGYSRGLATHHFGSKGAMMQRLVDTVTSQFRDAMVEESQSDSPIDQLRQLIDFYFHV
                     VSDLQPVNRARLVLWADAVAGPSEDIRPQMISADREFREEIEKRIQLAVTAGDVSDTV
                     DPHGLATVIIAMLRGVALQRVLDDHVDLVAAQHEVEQLLTSRLGLS"
     gene            complement(4355..4810)
                     /locus_tag="VFC01_03135"
     CDS             complement(4355..4810)
                     /locus_tag="VFC01_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZN78672.1"
                     /translation="MITDDLRQQRLAIIREHMDTEVTKEFDATLATFNGHPHYEIMAT
                     GQIYDGDDEVMGYYLTTRTAFPDQRHDNVRFHVADDAVIVEFDLLGTNLGEFYGLPPT
                     GKAFRVPLIAVFFFEGDRIVNERIYFDTASLVTQIGRGELLAMAGNVDG"
     gene            complement(4883..5302)
                     /locus_tag="VFC01_03140"
     CDS             complement(4883..5302)
                     /locus_tag="VFC01_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN78673.1"
                     /translation="MQVTSQTMVAAPIETVWDKLSNHVGMSQWGPGITVTMDRVGTDD
                     PNGVGAIRRIASPGPAPDIVEEVVAFEAPNLLGYRALSGTPFPGYTGEVRLTPAAPGT
                     HISYTISSTAKFPLVKAPLAVLCQMLLRLLARASKQK"
     gene            complement(5242..6093)
                     /locus_tag="VFC01_03145"
     CDS             complement(5242..6093)
                     /locus_tag="VFC01_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN78674.1"
                     /translation="MKVHHLNCGTMHPILCGKTVCHVLLVETDNDLVLIDAGFGSKDC
                     ADPARRLGPARRILRPALDHQEAAINQVERLGFRREDVRHIVITHFDPDHIGGISDFP
                     EAQIHVTTAEADSMLRAPSLNERIRFRAVQWAHGPKVIEHTPDGEKWRGFAAAKELTE
                     VSPGIALISLPGHTRGHACVAVDAGDRWVLHCGDAFFHHSTIDGTRMPRAIAAFETAT
                     AFDRKMVQQNHARLNELYGRREPDMFMVCSHDLTLYEQAKAPRESQCKSPRKPWSPHP
                     SKPCGTN"
     gene            complement(6104..6586)
                     /locus_tag="VFC01_03150"
     CDS             complement(6104..6586)
                     /locus_tag="VFC01_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HZN78675.1"
                     /translation="MVSLVVHALLGIAVVWFTVASNPKIFSRPPDGPRFSALEIAFWA
                     IGLASLPLCWYFNINYVYEYSANPFWGEGADWSQFISLGYANPAAASASADYTIVNVL
                     LLPLYTILDGRRRNIRRPWLFFVSSLFTSCAFAYAFYFATIERQRRHKIADIEVPSGV
                     "
     gene            6656..7261
                     /locus_tag="VFC01_03155"
     CDS             6656..7261
                     /locus_tag="VFC01_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78676.1"
                     /translation="MVRPAQTARSERTREALRRAALVRFIAQGVDETSAEQIAGDAGV
                     SLRTFYRHFASKHDLLFADYDAGVHWFRVALADRPADEPIIESVQAAIMAAPYDDWAV
                     TQIASLRAQELDPGRIVRHIRQLEADFADAIEDRLAGDNPPQAGTDERMLITVTARCI
                     AAAVFGAMEVWMLGNDRSLPELDRLCRAALKSLRTGLGDTD"
     gene            7316..8878
                     /locus_tag="VFC01_03160"
     CDS             7316..8878
                     /locus_tag="VFC01_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN78677.1"
                     /translation="MADYDAIVIGAGHNGLTAATLLQRAGLRTLCLDSKLYAGGMAST
                     VELFDGYKFEIAGSVQIPTSVAVSRELGLDELPNVELDVMSVSLRGVGDDPLIYYTDP
                     MKLMTHINDVHGAEAVNGMAGLLAWCQAPTRALGRFDAGKPPKTLDEMYACATTEFER
                     STITDLLFGSVTDVLDRYLPDKEKHGALRGMLSLLACNTTYRGPATPGTAAALAYGFA
                     VPDENALLVKKVRGGIGALTSHLCDVFTANGGELRLRSKVDEILVADGRITGVRLEDG
                     TTFTTPVVVSGIAPDLTINGLVDGAAVPADIRERFSRVDHRGSYLQMHFALDGIPEFA
                     SPYELLNDPAMQSNIGIFSTPEELQQQWEDCRRGIVPADPSIAVQIPSVNDPDLAPPG
                     KHAASAFSLWFPIEEGDAGYGEMKVEMGQRVIDKITRLAPNFESLILRHTTFTPKHMG
                     TMFGAPGGDYCHGLIHPDQIGTNRPGPKGYVDQPIPIDGLYLGSAGCHGGPGITFIPG
                     YNAAQAVLADAT"
     gene            8918..9898
                     /locus_tag="VFC01_03165"
     CDS             8918..9898
                     /locus_tag="VFC01_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN78678.1"
                     /translation="MALTVAVTGPTGDIGISAVEALEHAPEVERIIGMARRPFDPAAH
                     GWTRTEYLQGDILDRAAVDALVADADVVVHLAFIIMGSREESARVNLEGTRNVFEATV
                     AADRPCRLVYTSSVAAYGYHADNPMPITEDVPTRGSPEHYYSEQKAACESALVEVTAD
                     SSLDVYVLRPCIVAGPKAPALAEAMPWNQLPGPVKRVAQALPLLKPPFPDPGTPLQLV
                     HHDDVAAAIALAATTSAPPGAYNIAADGVLSMSDVAETLGARPFRLPRAAASAASEVI
                     ARLPFVPSALEWLHVGRTSVVMDTAKAKSQLGWTPKYSAAETLAALGAAI"
     gene            complement(9895..10059)
                     /locus_tag="VFC01_03170"
     CDS             complement(9895..10059)
                     /locus_tag="VFC01_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78679.1"
                     /translation="MNTKKPKTAESDVGAQGADDDVESDPAKGSDDRVDWSDEGGATP
                     SGPAGDTDDR"
     gene            complement(10056..11000)
                     /locus_tag="VFC01_03175"
     CDS             complement(10056..11000)
                     /locus_tag="VFC01_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZN78680.1"
                     /translation="MVKAGSESTLTVLVAFGANFAIAAAKTAAAAVSGSASMVAEAAH
                     SWADTGNEVLLLVANRRSARPADDRRPLGYGREVYVWSLLAAIGLFVVGASVSIWHGV
                     SELVGGEQAHGRDYALAYVVLAISFVLEGISFFQAVRQLRAEAHRLDRDLLTHALETS
                     DPTTRAVFAEDAAALIGIVIALGGIGLHELTGVAAWDAVGSILVGVLLGVVAVLLIDR
                     NRRFLTGEPGSADLRNAAITRVQQLPDVAEVRYLRLEFIGPKQLFLVASVDLTGDDAE
                     SSVARRLRRLERELETDPYVVDAVLTVSEPDDAGSQSR"
     gene            complement(11007..11354)
                     /locus_tag="VFC01_03180"
     CDS             complement(11007..11354)
                     /locus_tag="VFC01_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HZN78681.1"
                     /translation="MSADIDPRVPPSGTGCVECDAVGGWWVHLRRCAACGHVGCCDDS
                     LARHASAHWRETGHPIIRSFEPGEDWFWNFATNEYDDGPELAAPECHPVDQPAPGPSD
                     RVPADWVQQLRSR"
     gene            complement(11597..12046)
                     /locus_tag="VFC01_03185"
     CDS             complement(11597..12046)
                     /locus_tag="VFC01_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN78682.1"
                     /translation="MELWELAARERIRDTLARYNWSGDALRLDELAQSFSEDGELELR
                     GGEHVRGRAAIVELLSGVVAAPKPTAQAAGVKRIVRHNVTNIRFTEIRPDEARVACYF
                     TVFTEIGLDHYGRYRDVFVPVGNDWLIRHRFVSTDWSAPDSTMARTH"
     gene            12079..12525
                     /locus_tag="VFC01_03190"
     CDS             12079..12525
                     /locus_tag="VFC01_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN78683.1"
                     /translation="MATSDSREVVIEASPDEILDVIADVESTPTWSPQYQSAEVLEAY
                     DDGRPKRVKMKVKSAGISDEQVVEYTWTDNKASWTLISAGQLKSQDASYTLTPEGDKT
                     KVKFDITIDLSVPLPGFVLKRAMKGAMETATDGLRKQVLKVKKDGK"
     gene            13233..>15379
                     /locus_tag="VFC01_03195"
     CDS             13233..>15379
                     /locus_tag="VFC01_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN78684.1"
                     /translation="MRTIEAAISASAVSGVLVCGAAGVGKSRIAYEALAAAAAQGCET
                     RWTAGSASARAIPLGAFTAWAPTGVTDTFQLLRGVIDSLSAASSASNVILGVDDVHML
                     DELSTFVVHQIVQRGAAKVILTVRDGAPMPPAIQELWTAGRFDRLDLKQLSLDETAAL
                     LSAALEGPVDSDAAQRLWKLTQGNVLYLRNIVEQEVADGRIVHERGYWRWVGDPIMPP
                     GLVELIESRIGDLPTPVSDVIDVLAVGEPIDLEVLTRITDEAAVEEAETRGVIKLEPA
                     RRSIEVRVAHPLYGQVRRKRAAHIRLRRLRGLIATELAASTDRDDIRVVVRRATLSLD
                     SDLTPDADLLVRAAHGAVWLADLPLADRLAEAAIDAGAGPEPNLLRAHALSWLGRGEE
                     AEAVLADVRTDQLSDEDRARFAFLRSSNLMWALGDPVRAKEFIDEASRVAPARARTYI
                     DAFLTVYWFAMDQPAAAMQASKNIALDAVPVVGAEIAWALAQIFGDAGHTTDAVSAAQ
                     AGYTVATRCLDAPHMRFNIADAEVGALLLSGQIPGALDGAERIRQQAADLPGAAQLLG
                     AAVAGRAALGAGNLDSACSLLEQAADGLSVSHAIGWGYRYRIPHVTALAIRGLTDNAV
                     AALAALDKVQRKYRSLDYERSLAQAWVAASEGAVSEAIAVLLSAAERARATGRFAAEV
                     LCLQTATQFGDRTGAPRLRELESIVEGPRAALAA"
BASE COUNT         2710 a         4844 c         5085 g         2740 t
ORIGIN      
        1 cgatcggtgc ggtgttcaac aacgccggcg tcgccacggc ctccagcgtg ctcgacgcga
       61 tacccgagga cgaccaatgg ctgtggaaca tcaacttcca cggcgtggtc aacggcaccc
      121 gtgcgttctt gccgatcctc gtcgagcaga acgagggtgc gatcgtcaac acgtcgagtg
      181 tgttcggcct tgtcggcatg ccccaccaaa gcgcgtactg ctcagccaaa ttcgccgtcc
      241 gcggcttcac ggattccctt cgccaggagt tgcgcggcac cggcgtgacc gccatcaacg
      301 tgcaccccgg cggcatcaac accaacatcg tgcgcaacgc ccggtggcgc aaggatcccc
      361 acggacgtgg ccgcagccaa gaacagatgg ttcaggaatt cgccgcgatc acgctgacgc
      421 agccggacaa ggccgccgag atcattcacc gcggagtaga gcagggcaag gcgcggatcc
      481 ttgtcgggcc cgacgcctat ctgttcgaca cgcttgggcg gttggcgccg acgcgctact
      541 acgacattct cggtcggctc gaatccgtcc tgcggcgacg cagccagacg aacgtcgtgc
      601 ctgaggtggc cgtgccgtga ccgcggcggt gcacctgccc ccggggccac gctggccggt
      661 tcccctccaa ggccttgcat acatcgcgtc gcgccgccgc atgatgcagg ccttgcgcaa
      721 gcgctatggc agctcgttca ccgtgaggct gccgttcttc gggccgacgg tcgtcatcag
      781 cgatcccgca ctggtgaagc agttcttcca gacgcgaacc gatgtcgtgc gcaatgtgga
      841 acccaatctc gacctggtgc tgggccctgg ttcgacgttc gggttgcagg acgaacagca
      901 tcgccgacgc cgcaagctgc tggttccgcc gcttcacggc aagcggatgc gcgcgtacga
      961 ggatctcgtt gaggaagaaa cgctcaagga gatcgccacc tggcccgagg gccgggagtt
     1021 tgcggtactg ccgtcgacga accggatcac cctcaacgtg atcctgcgcg cagtgttcgg
     1081 cgccgagggt gccgagttcg atgcgctgcg caaggtgatg cccccgctgg tggaactggg
     1141 atcgcatctt gctttcctgc catggctgca ccgcgacctg ggtccatgga gcccgtgggg
     1201 ccgattcctc ggccagcgaa ggcaattcga cgagatcgtc gggtcactga tcgaccgcgt
     1261 cgacgccgac ccgaatatcg agcagcgcga cgacgtgttg tctctgatgt tgcaggctcg
     1321 ctacgaggat ggaacggcga tgtcgcgcag cgatctcgcc gacgaactgt tcacactgct
     1381 tgccgctggg cacgaaacca ccgcaacgca attggcgtgg gccgtcgaac ggctgcgcag
     1441 gcatccggcc gtgttgtcgc gactcgtcga cgaggttgat actggcagct cggatctgct
     1501 gcaggcgacc attcacgagg tgcagcgcaa tcggccggtc atcgacggtg cggctcggca
     1561 ggtcatcgcg ccgacaatgc cgttggggga gtgggtcatt ccgcacggct acaccgtcca
     1621 ggtcgacatc ccactgacac atcagaacgc ctatccggat cccgagcggt tcgacccgga
     1681 ccggttcatg gagtcgagcc cggatatgta ttcgtgggtg ccgttcggcg ggggcgcgcg
     1741 gcgttgcctg ggcgcggcgt tcgcgaacat ggagatgaat gtcgtgttac ggacgcttct
     1801 gcgcaagttt cggttcgtgc caacggcatc gcgacctgag cgattgcaca gccgtggcgt
     1861 agccttcgca ccgagtggcg gcggccgcgc cgctgtctat cgacgtatcc acacggccgc
     1921 gtcggacaac ctgcggaaga gcgcgcgcgt tagcgtttga catcatgcgc gcggtcgtca
     1981 tcaccaagca cggagacctc tcggtcctgc aggtgcagga acgtcccgat ccgccaccgc
     2041 cggggccggg gcaggtgtgc atcacggtgc gcgcggccgg ggtcaacttc gccgaccacc
     2101 tcgcccgcgt cgggctgtat cccgatgcgc cgaagccacc catggtcgtc ggttacgagg
     2161 tggccggggt catcgaggct gtcggcgacg gcgtcgatgc tgcacgggtg ggggagcggg
     2221 tgttcgcggg cacccgattc ggcgggtatg cggagatcgt caacgtgcaa gcgctcgacg
     2281 cggtgccaat cccagactcg ctgactttcg agcggggtgc ggccatcccg gtgaactacg
     2341 ccaccgcgtg ggcggcgctg cacggctacg ggtcgctgcg tcccggcgaa cgtgtgctga
     2401 ttcatgcggc ggcaggtggt gtcggcatcg cggcgatcca gctggccaag gccgccggag
     2461 ccgaggtgca tggcaccgcg tcaccgggta agcacgcccg gctggccgag ctgggtgtcg
     2521 accgtgccat cgactaccgc cgcgacggct ggtggaaagg cttgccgccg tacgacattg
     2581 tcctcgacgc gatcggcggc aagtcgctga agcgctcgta tgacctgctg cgccccgggg
     2641 gccggcttgt cggctacggc gcttcggcca tgcaacaggg cgagaagcga tcgctgcgca
     2701 ccgcgctacc gcaactgttg tcgatggtgc gcggtttcaa tttggtcaag cagctgtcgg
     2761 agtccaagac cttgatcggg ctgaacatgc ttgcgctgtg ggacgatcgc ggcacgctgc
     2821 agccgtggat cgagccgctg tcgaaggcga tgtccgacgg tgtggtccaa ccggtagtcc
     2881 acgcggcggt gccgttcgcc aacgccggtg aagcccatcg catcctggcc gcccgcgaga
     2941 acgtcggcaa ggtggtgttg gtgccttaag cggtggcttg cgctcgctcg agaagtgtcg
     3001 ggtcgtgcgc gcagacgatc atcaggtccg ggtcctgacg ccgatacagc tcgacgaggc
     3061 gagcatggtt ggccagcacc gcctttcgat cgaacgcgac catggactcc atcgccgtca
     3121 gcgcccgcgg cactgctgtg ccgtcgagcg tgccgtagtg atagaaagca tcgccggcgt
     3181 gtagcaccca tcgatggccg gcatcgacgg ccacgcaggc gtgcccacgg gtgtgcccgg
     3241 gcagcgaaac gagcgcaatg cccggcgaga tctcgtcgag ttccttggcg gcggcgaacc
     3301 cgcgccactt ttcgccgcgt gggtcgtgct ccacgatgtt gggttcgtgc gcccactgcg
     3361 cggagcggta gcgaatcttc tcctgccgcg acggggccca aatcgcgccg agcgcctcgg
     3421 cggcggtgag gtggatctgc gcgtgcggga agtcagaaag cccgcccgcg tggtccatat
     3481 ccatatgcgt gatgacgatg tgccgcacgt catctcgcac gaagccaagt cgttttatct
     3541 gatgcgcgac ggtttcctcg tgagccagga tcgctcggac catgcgccgg gtcgggccca
     3601 cccttctcgg atcgtcgcag tcatgggtcc caaatccgct gtcgaccaag accagcccgt
     3661 tgttgctttc gatcagcagc acgtggcaga ccattggggc ggtcggcatc ctcatggtgc
     3721 cgcagttgag atggtggacc ttcatgacaa tcccagccgc gacgtgagca gctgctcgac
     3781 ctcatgttgc gccgcaacga gatcgacgtg gtcgtcgagg acgcgctgca atgccacacc
     3841 ccgcaacatc gcgatgatca ccgtcgccag accatgcggg tcgacggtgt cgctcacgtc
     3901 cccggcggtg acggccagct gaatgcgctt ctcgatctct tcgcggaact cacggtcggc
     3961 ggagatcatc tgcggcctga tgtcctcgga cggcccagcg accgcatcgg cccacagcac
     4021 cagccgagcc cgattcaccg gttgcaggtc ggagaccacg tggaagtaga agtcgatcaa
     4081 ttgccgcaat tggtcgatcg gtgaatccga ttggctctct tcgaccatcg cgtcgcggaa
     4141 ctggctggtg accgtgtcga cgagtcgctg catcatggca cccttggacc cgaaatggtg
     4201 ggtcgccagg ccgcggctgt agccggcgcg ctcgccgaca ttggcgagcg tcaccttgcc
     4261 cgggccgacc tcaccgacga gctctgcggc ggcctgaacc agccgccgtt cggcttcgat
     4321 gcggcggtgc tcctgcgtgc gcggcaggct ggagtcagcc atcgacattc ccggccatgg
     4381 cgagcagttc gccgcgaccg atctgagtga ccaggctggc cgtgtcgaag tagatgcgtt
     4441 cgttcacgat ccgatcgcct tcgaagaaga acaccgcgat gaggggaaca cgaaacgcct
     4501 tgcccgtcgg cggaagtccg tagaactcgc cgagattcgt tccgagcaag tcgaattcga
     4561 cgatcacggc atcgtcggcg acgtggaagc gcacattgtc atggcgttgg tcggggaagg
     4621 cggtgcgagt cgtcaggtag tagcccatca cctcgtcgtc accgtcgtag atctgaccgg
     4681 tggccatgat ctcgtagtgc ggatggccgt tgaacgtggc cagcgtggcg tcgaactcct
     4741 tcgtcacctc ggtgtccatg tgctcgcgga tgatcgcgag gcgctgttgc cgtaggtcgt
     4801 cggtgatcac gagcactcct ccataacttg cttgttgacg aacaagttac ggtacgaggt
     4861 ggtaccgcgt caaggtgtga gcctattttt gcttggacgc gcgagcgaga aggcggagca
     4921 acatctggca gagcaccgcc aacggcgctt taacgagcgg gaatttcgcg gtggaggaga
     4981 tggtgtagct gatgtgggtg cccggggcgg ccggggtcag ccgcacctct ccggtgtaac
     5041 ccgggaacgg cgtgcccgaa agggccctgt agccgagcag gttcggagcc tcgaaggcca
     5101 cgacctcctc gacgatgtcg ggggccgggc ccggcgacgc aatacgccgg atggcgccga
     5161 cgccgttggg atcgtctgtg ccgaccctgt ccatcgtcac cgtgatgccg ggaccccact
     5221 ggctcatccc gacgtggttg ctcagtttgt cccacacggt ttcgatgggt gcggcgacca
     5281 tggtttgcga ggtgacttgc attgtgactc cctaggggct ttggcctgct cgtacagcgt
     5341 caagtcatgt gagcaaacca taaacatgtc gggttcccgg cgtccataca gctcgttgag
     5401 tcgagcgtgg ttctgctgca ccatctttcg atcgaatgca gtcgccgtct cgaatgcggc
     5461 gatcgcacgc ggcatccgtg ttccgtcgat tgtgctgtgg tgaaagaagg cgtcgccgca
     5521 gtgcagcacc cagcgatcgc cggcgtcgac cgccacacac gcgtggccgc gggtgtgccc
     5581 ggggagcgat atcagcgcga tacccggcga tacctcggtg agttccttgg ccgcggcgaa
     5641 tccgcgccac ttctcgccgt ccggggtgtg ctcgatgacc ttggggccgt gcgcccattg
     5701 caccgcgcgg aagcggatcc gctcgttgag ggacggggcg cgcagcatgc tgtccgcctc
     5761 cgcagtggtg acgtgtatct gggcttcggg gaaatcggag atgccgccga tgtggtcggg
     5821 gtcgaaatgg gtgatgacga tgtggcgcac gtcctcgcgg cgaaagccaa gccgctcaac
     5881 ctgattgatg gcggcctcct gatgatcgag cgccgggcga agaattcgcc tcgccggacc
     5941 caggcggcgc gcgggatcgg cgcagtcctt cgacccgaag ccagcgtcga tgagcaccag
     6001 atcattgtcg gtctcgacga gaaggacgtg gcacaccgtc ttgccgcaca gaatcgggtg
     6061 catcgtcccg cagttgaggt ggtgaacctt cacacgtgca gtgtcacacg ccagacggca
     6121 cctcgatgtc ggcaattttg tgccgacgct gccgctcaat ggtcgcgaag tagaaggcgt
     6181 acgcgaatgc gcagctggtg aagaggctcg atacgaagaa caaccacggg cggcggatgt
     6241 tacgccgccg cccgtcgagg atggtgtaca acggcagcag aagaacattg acgatcgtgt
     6301 agtccgcgct ggccgaagct gcggcgggat tggcgtaacc cagcgaaatg aattgggacc
     6361 agtcggcccc ctcgccccag aacgggttcg ccgagtactc gtacacatag ttgatgttga
     6421 aataccagca cagcgggagc gacgcgagac cgatcgccca gaacgcaatt tccagggcgg
     6481 agaaccgcgg accgtccggg gggcgggaaa agatcttcgg attcgatgcg acggtgaacc
     6541 agacgacggc gatgccgagg agcgcgtgca cgacgaggga gaccattccc ggagtctcgc
     6601 caccgatctg agttttgtca atattgacac aaccgagttg gggtacctta cgcacatggt
     6661 ccggcctgcc caaacggcgc gcagcgagcg tacgcgcgag gctttgcggc gcgcagcgct
     6721 ggtgcggttc atcgcgcagg gggtcgacga gacgtcggcc gaacagatcg cgggcgacgc
     6781 cggcgtctcg ctgcgcactt tctatcgcca cttcgcgtcg aagcacgatc tgttgttcgc
     6841 cgactacgac gcgggcgtcc actggttccg cgtcgcgctg gcggatcgcc cagccgatga
     6901 gccgatcatc gaatccgtgc aggcggcgat catggctgcg ccgtatgacg attgggcagt
     6961 cactcagatc gcctcgttgc gtgcccagga actcgatccc ggtcggatcg tgcggcatat
     7021 ccgccagctg gaggccgatt tcgccgacgc gatcgaggat cggcttgccg gtgacaatcc
     7081 gccgcaggcc ggcaccgacg agcggatgct catcacggtc accgcacgtt gcatcgccgc
     7141 cgcggtgttc ggcgcgatgg aggtctggat gctcggcaac gaccggtcac tgccggagct
     7201 cgaccggctc tgccgcgccg cgctgaaatc gttgaggacg gggcttggcg acacggacta
     7261 acgcgtagtt tcgtcaatat tgacaaaact acgccgtgcg tgtcagcctc agccgatggc
     7321 ggactacgac gcgattgtca tcggcgcggg ccacaacgga ctgactgcgg ccacacttct
     7381 gcagcgtgcg gggttacgca cgctctgcct cgactccaag ctgtacgccg gcgggatggc
     7441 gtcgacggtg gaactgttcg acggctacaa gttcgagatc gccggctcgg tgcagattcc
     7501 gacgtcggta gcggtgagcc gcgaactcgg gctcgacgag ctgccgaacg tcgagctcga
     7561 tgtgatgtcg gtatccctgc gcggcgtcgg cgacgatccc ctcatctact acaccgaccc
     7621 gatgaagctg atgacgcaca tcaacgacgt gcacggtgcg gaggccgtca acgggatggc
     7681 cggcctgttg gcctggtgcc aagcgccgac gcgggcgctg ggacgtttcg acgccggcaa
     7741 gcccccgaaa accctcgacg agatgtacgc ctgcgcgaca accgaattcg agcgctcgac
     7801 gatcaccgac ctgctgttcg gctcggtcac cgacgtgctc gaccggtacc tgccagacaa
     7861 ggagaagcac ggcgcgctgc gaggcatgct gtcgctgctg gcgtgcaata ccacctaccg
     7921 cgggccagcg actcccggca ccgcggcggc gctcgcgtac ggattcgctg tcccggacga
     7981 gaacgcgctg ttggtcaaga aggtgcgagg cggtatcggg gcgctcacgt cgcacctgtg
     8041 cgacgtcttc accgcgaacg gcggtgaact acggctgcgc agcaaggtcg acgagatcct
     8101 ggtcgccgac gggcgtatta ccggggtccg cctcgaagac ggcacgacat tcactacacc
     8161 ggtcgtcgtt tcgggcatcg cacccgacct cacgatcaac ggattggtcg acggcgccgc
     8221 cgtgccagca gacatcaggg agcggttctc gcgcgtcgac caccgcggca gctacctgca
     8281 gatgcacttc gcactcgacg gcataccgga attcgcatcg ccctacgaat tgctcaacga
     8341 ccccgcgatg cagtcgaaca tcgggatttt cagcacgcct gaggagctgc aacaacagtg
     8401 ggaggattgc cgacgcggca ttgtgccggc cgacccgtcc atcgccgtgc agattccgtc
     8461 cgtcaacgac cccgacctgg caccgcccgg caagcacgcg gcgtcggcgt tttcgctgtg
     8521 gttcccgatc gaggagggcg acgcaggtta cggcgagatg aaagtggaga tggggcaacg
     8581 ggtcatcgac aagatcaccc ggctcgcgcc caacttcgaa agcctgatcc tcaggcacac
     8641 cacattcacg cctaagcaca tgggcaccat gttcggcgca cctggcggcg actactgtca
     8701 cgggctgatt catcccgacc agatcgggac caaccggcca gggccgaaag gctatgtcga
     8761 ccaaccgatt ccgatcgacg ggctgtacct cgggagtgcc gggtgccacg gcgggccagg
     8821 gatcacgttc atccccggct acaacgccgc acaggcggta ctcgccgacg cgacctgacg
     8881 cgtttcaaga tctcggcggc ggggaacgcc gacgagtatg gctctcacag ttgccgtcac
     8941 gggtccaacc ggcgacatcg ggatctcagc ggtcgaggcg ctcgaacatg cgcctgaagt
     9001 cgagcgaatc atcgggatgg cccggcgtcc cttcgatccg gctgcgcacg ggtggacccg
     9061 aacagaatat ctacagggcg acatcctgga ccgcgccgcg gtcgacgcgt tggtcgctga
     9121 cgccgacgtg gtggtccatc tggccttcat catcatgggc tcgcgtgagg aaagcgcgcg
     9181 cgtcaacctc gagggcaccc gcaacgtctt cgaagcgacg gtggcagctg accggccctg
     9241 tcgcctggtg tacacctcgt cggtggcggc gtacggctac cacgccgaca atccgatgcc
     9301 gatcaccgag gatgtaccga cccgcggctc acccgagcac tactactccg agcagaaggc
     9361 cgcctgcgaa tccgcacttg tcgaagtcac ggccgactca tcgcttgatg tctatgtgct
     9421 gcggccgtgc atcgtcgccg gaccgaaggc gcccgcgctc gccgaggcga tgccatggaa
     9481 ccagctgccc ggtccggtga agcgggtcgc gcaggcgctg ccgttgctca agccgccgtt
     9541 ccccgatccc ggtacgccgc tgcagctggt gcaccacgac gacgtggccg ccgcaatcgc
     9601 cttggcggca acgacttccg ctccaccagg tgcctacaac atcgcagccg acggagtgct
     9661 gtcaatgtct gacgtggccg agacacttgg cgcgaggccg ttccggcttc cccgggcggc
     9721 ggcgtcggcc gcgtcggagg tcatcgcgcg tctgccgttt gtaccgtcgg cgttggagtg
     9781 gttgcatgtg ggccgcacgt ctgtggtgat ggacaccgcc aaggcaaagt cacagctcgg
     9841 gtggacaccg aaatactctg ccgccgagac actggcggcc ttaggtgccg cgatctagcg
     9901 gtcgtcggta tcccctgcgg ggccgctcgg cgtggcgcca ccttcgtcgg accagtcgac
     9961 gcgatcgtcg gatcccttgg cgggatcgct ctcgacgtcg tcgtccgcgc cctgtgcgcc
    10021 gacgtccgat tcggcggtct tgggcttctt cgtgttcacc tgctctgact acccgcgtcg
    10081 tcgggctcgg aaaccgtcag gacggcatcg accacatacg ggtcggtttc caattcgcgc
    10141 tccaagcggc gcagccgacg cgcgaccgac gactccgcat cgtcgccagt gaggtccacg
    10201 ctggcgacga ggaaaagctg tttggggcca atgaattcca gccggaggta ccgcacctca
    10261 gccacgtcgg gtagctgttg aacccgagtt atggccgcat tccgtaggtc agcggaaccg
    10321 ggttcaccgg tcaggaaccg gcggttgcgg tcgatcagca gtaccgcgac cacgccgagc
    10381 agcacgccca caaggatcga gcccaccgca tcccacgctg ccaccccggt gagttcgtgc
    10441 agcccgatgc cgccaagggc gatgacgatg ccgatgagtg cagcggcgtc ctcggcgaac
    10501 accgcgcgcg tggtcggatc cgacgtctcc agcgcgtgcg tgagcaggtc acgatcgaga
    10561 cggtgtgcct cggcccgcag ttgcctgacg gcctggaaga acgagattcc ttccagtaca
    10621 aaggaaatgg ctaacacgac gtaggccagc gcgtagtcac ggccgtgggc ctgctcacca
    10681 ccgacgagct cgctgacgcc gtgccagatc gacaccgacg cgccgacgac gaacaggcca
    10741 atggcggcga gcaacgacca cacgtaaacc tcgcggccat agccaagtgg tcggcgatcg
    10801 tcggcgggac gcgcactgcg ccggttcgca accagtagca gcacttcgtt gccggtgtcg
    10861 gcccaggaat gcgccgcttc ggccaccatc gacgccgaac cggacaccgc ggcggcagcg
    10921 gttttggcgg ccgcgatcgc gaaatttgca ccgaacgcga cgaggacagt cagtgtgctt
    10981 tcgctgccgg ccttgaccac gagcgctcag cggctacgca gttgctggac ccaatccgcg
    11041 ggcaccctgt cgctcggccc gggtgccggc tgatcgaccg gatggcattc gggcgcggcg
    11101 agttcgggcc cgtcgtcata ctcgttcgtt gcgaaattcc agaaccagtc ttcgcccggt
    11161 tcgaaggacc ggatgatcgg atgcccggtt tcgcgccaat gcgccgacgc gtgccgcgct
    11221 agcgagtcgt cgcagcagcc gacgtggccg cacgcggcgc agcggcgaag gtggacccac
    11281 cagcccccga ccgcatcgca ttcgacgcaa ccggtgccgc tcggtggaac cctgggatcg
    11341 atgtcggcgc tcatgtgacg agcctaccga ggcggtcgca gcgtgctcgc aagaccaaaa
    11401 ttaacacgat ttcatcgata tgagcgaaac caaatgcgtt ggggcaacca gcgaattccc
    11461 tcagttgagg gtttcgtttg tgaatctggg gggtgaagag cgcgctcgcc aactgggtcc
    11521 gcggcgcctt agcgtgtgcc gttgactccg cgcgtatggt ggcgcgtact cgcactttgt
    11581 cgccattaac gcatagtcaa tgcgtcctag ccatcgtcga atcgggtgcg ctccagtcgg
    11641 tcgagacgaa gcgatggcgg atcagccagt cgttgcccac gggtacgaag acgtctcggt
    11701 agcgcccgta gtgatcgagg ccgatctcag tgaagaccgt gaagtagcag gcgactcgcg
    11761 cctcgtccgg gcgtatctcg gtgaagcgga tgtttgtgac gttgtggcgc acgatccgct
    11821 tcactcccgc cgcttgcgcg gtcggcttcg gtgcggcgac gacgccactt agaagctcga
    11881 cgatcgcggc gcgtccacgt acgtgttcgc cgccgcgcag ctccagctcg ccgtcctcgc
    11941 tgaaactttg agcaagctcg tcgagtcgca gcgcgtcgcc cgaccagttg tagcgggcca
    12001 gtgtgtccct gatgcgttca cgggccgcca gctcccaaag ctccatgacg caaaccgtaa
    12061 accagctaag gtcgcgccat ggctaccagc gattcccgtg aggtcgtgat cgaggcaagt
    12121 cccgacgaga ttctcgacgt catcgccgac gtcgagtcga caccgacgtg gtctccgcag
    12181 taccagagtg ccgaggtcct cgaggcctac gacgatggca ggcccaagcg agtcaagatg
    12241 aaggtcaaat ccgcgggcat ctcggacgaa caggttgtcg agtacacatg gacggacaac
    12301 aaagcgagtt ggaccctgat ctccgcgggt cagctgaagt cgcaggacgc cagctacacg
    12361 ctgaccccgg agggggacaa aaccaaggtc aagttcgaca tcacgatcga cctatcggtg
    12421 ccgctgccgg gattcgtgct caagcgggcg atgaaggggg cgatggaaac cgccaccgac
    12481 gggctgcgca agcaggtgct gaaggtcaag aaagacggca agtaattcga cagtggaccg
    12541 cccgcaccgt acgaaacgcg atgcgggcgc cacgaatcgc cccttcttta gcgggggcag
    12601 tttcccggga agttcgggga gcttgggtcg gtggcggcgg tgtgcccgcg gttgcaccca
    12661 ggtccggaag ccttgacgcc cattcctaca ggggtcttgt ccgggtatac atccggatag
    12721 aagacatcca acaaggtccc gtaggcctga ggaatgctgc aatgcgtggt gcaaacagta
    12781 tcctttagtt gactggttat cgacccggga ggattcggct cgagagggcc gtttgggtcc
    12841 ggaattccct gatttgcggc tatctcgttg atcctctgtg gcataccccc ttggcctatt
    12901 ccgccatcct tgcccgtgtc gtcgggcggg tctgcccatg ccattccggc gaggggaaca
    12961 gacacggccg ctgctgcggc ggcggtgacg aacagtttct tgagcatggc tcctcctctc
    13021 gataactgcg agcgcgagtg ctcacgtcag cgatcgtcag ggtctgccga tgagctggat
    13081 tcagtagtcg agccctgcac tttgcgaagg caatcgcccg actactgcag ctgactaggg
    13141 cgaagtcgcc ataactgcac gttcgcgagc ccaggcctct taggctcttt aaggtgcgtt
    13201 tgtcgtggcc gttaattggc cgcaccgagg agatgcgaac catcgaggcc gccatctccg
    13261 cctcggctgt ctctggcgtc cttgtttgcg gggcggcggg tgtggggaag agtcggatcg
    13321 cctatgaggc gttggctgcc gccgctgcgc agggctgtga aacccgatgg acggcgggca
    13381 gcgcgtctgc gcgggctatt ccgctgggcg ctttcaccgc gtgggcgcca acgggtgtca
    13441 cggatacgtt tcagttgctg cggggtgtga tcgactcgct gtccgccgca tcttctgcca
    13501 gcaacgtgat cctgggtgtc gacgacgtac acatgctcga cgaactgtcg acttttgtgg
    13561 tgcatcagat cgtgcagcgg ggcgcggcga aagtgatact caccgtccga gacggtgcgc
    13621 cgatgcctcc tgcgatacag gagctatgga cggctggccg attcgatcga ctcgacctca
    13681 agcaactgtc gctcgacgaa accgccgcgc tgctgtcggc agcattggaa ggtccggtgg
    13741 attccgatgc ggcacagcga ctatggaagc tgactcaagg caatgttctg tatctgcgta
    13801 acattgtcga gcaggaagtt gccgacggcc ggatcgtgca cgagcgcggg tactggcgtt
    13861 gggtgggtga tccgatcatg ccgcccggcc tggtcgaact catcgaatcg cgcatcggtg
    13921 acctgcccac gccggtcagt gatgtgatcg acgtgttggc tgtcggcgag ccgatcgacc
    13981 ttgaggtcct gacgcggatt accgatgaag cggcggtgga ggaggccgaa acccgcggtg
    14041 ttatcaagct ggagcctgcc cgtcgcagca tcgaggtgcg ggtggcgcat ccgctttacg
    14101 gacaggtacg ccgcaagcgg gcagcgcaca tcaggttacg acggctgcgg ggattgattg
    14161 ccaccgaact cgctgcatcc actgaccgcg acgacatacg agtcgtcgtg cgccgcgcga
    14221 cgttgagtct ggactccgac ctcaccccag acgccgattt gttagtgcga gcagctcatg
    14281 gcgcggtctg gctggcagac ctgccactcg cggatcgtct cgcggaagcg gcaatcgatg
    14341 ccggagcggg accggagccc aatctccttc gcgcccatgc cttgtcgtgg ctaggccgcg
    14401 gcgaggaggc tgaggctgtg ctcgcagacg tccgcaccga ccagttatct gatgaggacc
    14461 gcgccagatt cgcgttcctg cggtccagca acttgatgtg ggctcttggt gatccggtga
    14521 gggccaaaga attcatcgac gaggcgtcgc gcgtcgcacc tgcccgtgcg cgcacctata
    14581 tcgatgcctt cctgaccgtg tattggttcg cgatggatca gccggctgcg gcgatgcagg
    14641 cgtcgaagaa tattgcgttg gacgctgtgc ctgtcgttgg cgcggagata gcctgggcgc
    14701 tcgcccagat attcggggac gccgggcaca caaccgatgc cgtttcggcc gcgcaagcgg
    14761 ggtataccgt cgcgacacgt tgtcttgacg ctccacacat gaggttcaac atcgctgacg
    14821 cagaagtcgg cgcactgctg ttatcgggcc agatcccagg ggctttggac ggagccgagc
    14881 gcatacgcca gcaggcagcc gatcttccgg gagcggccca attactcggt gctgccgtcg
    14941 cgggtcgggc tgctcttggg gccggaaatt tggatagcgc atgctcgctg ctggagcaag
    15001 cggcagacgg gctgtctgta tctcatgcca tcggctgggg ataccgttat cgcatacccc
    15061 atgtgacggc actcgcgata cgtggtctga ctgataatgc cgtcgcggcg cttgccgcgc
    15121 tcgacaaggt ccaacgtaaa taccggtctc tggactatga acgaagcctg gcgcaggcct
    15181 gggtggccgc cagcgagggc gccgtcagcg aggcgatcgc cgtcctatta tcggctgccg
    15241 aaagagccag agcaacaggg cgattcgcgg cggaggtgct atgcctgcag accgctaccc
    15301 agtttggcga tcgcacaggc gcgcctaggt tgcgcgagct cgaatcgatc gtcgaagggc
    15361 cacgagcagc gctagccgc
//
LOCUS       DASRJA010000070         8968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3044756,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000070 DASRJA010000000
VERSION     DASRJA010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3044756"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VFC01_03200"
     CDS             complement(<1..653)
                     /locus_tag="VFC01_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HZN78685.1"
                     /translation="MSSTASSGAVVVSPTERLEVLFEELAELAGQRNAIDGRIVEIVA
                     EIDRDQLCGATGARSVPALVAWKMACSPGNAHTIATVAGRLEEFPRCAQGMREGRLSL
                     DQVGVIAARAGEGSDEHYAALAAVATVNQLRTAVKLEPRPEPEPRPEPDARPEPHPSI
                     TKTSDEEFSCWRIKLPHPDAAKFDAALASHRDALIAEWKRDHGNGDRACDHAPPLPNT
                     "
     gene            complement(1079..2227)
                     /locus_tag="VFC01_03205"
     CDS             complement(1079..2227)
                     /locus_tag="VFC01_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78686.1"
                     /translation="MNHERRARKAKQIRRDERRARKRNVNGPADTVFRDAIRGALAGG
                     HPLSLLSLASMVIHVAKPKPPILVKSGRCDTNYLHPVLDGLIGVRNRETTVLLAVIAE
                     LLVDDPAPQLRCRNELAERGEYLPRWITALPQVEVYRAVRRTNVFGDVDELVIGMRLE
                     GGHELTIAVQIDHNMLSSVADAGVVPEPIDEALARVAESSSDTKVVETRLADARVWIE
                     DALSKPTLAPESDTWPLYRALVQWLVGRLPEGGEHQPPAVESESNEQLCDRFFATPSA
                     APFTDSSHRELLLELFETGTGDPLRWSTARVRSAIGSARYVEDRVPLDVALDAPDLLR
                     AFIPYAHAQSGIRDELTSRTLAMVDALRSSYKREVLRQAKYWGLDDAV"
     gene            complement(2271..3938)
                     /locus_tag="VFC01_03210"
     CDS             complement(2271..3938)
                     /locus_tag="VFC01_03210"
                     /inference="COORDINATES: protein motif:HMM:NF045160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78687.1"
                     /translation="MPLSETTLLEVAGDLVYARGEDYVRYVRGLRTTDFKAYASIQAK
                     NVYQVELDWSGPLPDGSCTCPHYADGNFCKHLVATGLAAIDTGRVAVDDTAGSAAEAA
                     LEAVVQAMDVDELRELVMTLAQRDDGVRRLLEIRATTAAGDNAHAKAALEAYVRNTLA
                     FRGFIDYRRSFEVAGVASEMLDELENHLNRGAAEVARPALLRAVTRLRKMIEHIDDSS
                     GSIGDQCQRAADLYARACRLGEPDPVKLATWLVKFRADSPGWPHLVLADFVEAFDEHA
                     LATYRRAVAALDRKLEGQDHWKRFEVDAMLLELADHDGNVDQAVDLLCQREHPQYGAI
                     VDRLRAAGRTEDAVAWIDRAVAEGRISSHGGGNSYWLSPDHVAETYLGLGRIDDAVAV
                     LRADFVRQPSVGAYRVLLDFASGVDRVDTERSWALDHARQLAASDRFAAGAVLVQLYL
                     SDGDIDAAWQAADRYGPGWAWRELADRGAEVRPVEAADLYRPQLENDLRYPNSKLYPD
                     IAARLATMAELYERGGRSDDFASFIAQIRQDYGRRPSLMKALDAKGL"
     gene            4123..4737
                     /locus_tag="VFC01_03215"
     CDS             4123..4737
                     /locus_tag="VFC01_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZN78688.1"
                     /translation="MARTNLSMSISADGYVAGPDQSEQNPLGVGGLAVHNWHIGPNTD
                     HPVNRQVMSDMMDGMGATIMGRNMFGPIRGEWADSDWRGWWGDTPPYHCPVFVLTHYA
                     HDPIEMNGGTTFHFVTDGIESAYAQAEAAAGGKDISIAGGASCARQAINAGLVDEIDL
                     QVAPVILGSGERLFDGFQPGIPQLELVRVLQAPGVAHLRYRVTR"
     gene            complement(5053..5424)
                     /locus_tag="VFC01_03220"
     CDS             complement(5053..5424)
                     /locus_tag="VFC01_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78689.1"
                     /translation="MNIIRVELLPDFMWGDDVVLLAMDGAGVATFADALNDAVRLGSS
                     RLEHGGVMHEFVMQAGAADIEMGEDRVVWRLDRTKAVEIADDLTAIREVDGPGHNYVD
                     ISSPTKTLVLSRDEYVSVNPS"
     gene            5873..7042
                     /locus_tag="VFC01_03225"
     CDS             5873..7042
                     /locus_tag="VFC01_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78690.1"
                     /translation="MAAISPVPSLSQIRAWDTEHLVDAATTWRTTADHWHGSFSQVAS
                     HMPAPGGTPWEGAGSETAQLRASTDAMKVDEIAVSLRSASTVAGNGADEIDGKRQSVL
                     KAIEDAESAGFSVGEDLSVTSRYADLTPDAEVARQAQVEALAADIHAKATALAAADHE
                     VAAKLASAASGVRDVTLCESPGADTGVDATQQEPHVLAAGWKQGPSDNPAPPPPSPPP
                     RVRGLPPEGLQPPVSGTLTPGPASRPSEQRVGGQSLWDEKGGEWRYYPGNKHHNPHWD
                     YNPHDVPNARWQNVPINNLPPVKGGPAPVEAAPPRPAVPASPPAEAAPPRPAVPVPPP
                     AEAPPIRGGPTGGLPLGGGALPDGALPHLVEPPEGAAGSPDLPVIGDGIADVPEA"
     gene            7064..7447
                     /locus_tag="VFC01_03230"
     CDS             7064..7447
                     /locus_tag="VFC01_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78691.1"
                     /translation="MTYDSQRGQNAYERLRAEVLTSGLSDWVSIAEVQQIITHFQLAE
                     TDSERQDLVLRAIRSLLEDCLMQIGELPGPDGTFPAWEPIDVAMDRLRERFVDHYAEP
                     TSWDYSIWLGLTEAGRSVAEKLRAG"
     gene            7594..8610
                     /locus_tag="VFC01_03235"
     CDS             7594..8610
                     /locus_tag="VFC01_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78692.1"
                     /translation="MQQDWFADLTGFRETSYDATREQLFVEGDELVSTVSGKRYGIGT
                     LEVPTMAELRGRVEVPSDGCTTVRCVAGEARSMHADPELEGALFQVASQFNLLEMTGP
                     SITPEDGVTRYAGDHTQGPACAIAAGAATIYRNYFAPVDGEKGQTRERQIDALAAVGE
                     ALSSKLGWPVSELWQMRNGYALCTTKGLDGITRLLADGDEDSRDELRGRLAIGLHRNV
                     EVTDVREGARRLVSQAFCSALPVAYGSGPSTAWEAFARLVLEAAYEATLIAAVEQSSA
                     GGSNIVLLTRVGGGAFGNADEWIDDAIVRALAIVEQAGLDIRLVSHGRIHDSNRAIAE
                     RFNG"
     gene            8603..>8968
                     /locus_tag="VFC01_03240"
     CDS             8603..>8968
                     /locus_tag="VFC01_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HZN78693.1"
                     /translation="MDENTSWPHDAILHAWWVEPGRLLAGEYPGARTASKAAVKVRLL
                     IDAGVHSIVDLTTPDDHLASYEEALRDAAEKAGRPVHHFAYPIADMGVIHREGYDEIV
                     ARVRGEMDSGRVVYVHCWGG"
BASE COUNT         1672 a         2856 c         2844 g         1596 t
ORIGIN      
        1 gtgttcggca acggcggtgc gtgatcgcag gcgcggtcgc cattgccgtg atcgcgcttc
       61 cactcggcga tcagcgcatc acgatgagac gccaaggccg cgtcgaactt cgccgcgtcg
      121 gggtgcggaa gtttgatccg ccagcagctg aactcctcat cggaggtctt ggtgatcgag
      181 gggtgcggtt caggccgggc atcgggttcg ggccgcggtt cgggttcggg tcgcggttcg
      241 agtttgaccg cggtgcgcag ctggttgacc gtcgcaacgg cggccagcgc cgcgtagtgc
      301 tcatcggatc cctcgcccgc ccgggccgcg atgaccccga cttgatccag cgacagccgg
      361 ccctctcgca tgccttgggc gcagcgggga aactcctcca gccggcccgc gacggtggcg
      421 atcgtgtggg catttcccgg cgagcaggcc atcttccagg ccaccaacgc cggcaccgac
      481 cgcgcgcccg tagcaccgca caactggtcg cgatcgatct cggcgacgat ctccacgatg
      541 cgcccatcaa tcgcgttgcg ctgaccggcc aactccgcca actcctcgaa caacacctca
      601 agacgctcgg taggactcac caccacggca ccagaggatg cggtcgaaga catgacgtca
      661 tcatcgcaga ggggtacgac aagtctcgtc agcgccaaat ctgcgccggg atgtggccgt
      721 catcaatcca caaggagtag agcctgattg gcggccaaac gctgctcgcc gctgctcgat
      781 tgtggccgtc tgaaggtgat gcaacccgga ccactacgcc tgttaatcct gactcggata
      841 acggcagcgt tagtccgagc tagcaggtct atgaaaccag cccaagaagc gtggccaccg
      901 catggccagg aatcccggcg ttgggcacga acacaccgag gtggcacaca agacgatcgg
      961 acgctgcgct caatcgtcgt ctcgttgcct gcattcatcg cgaaccgcac cgccgatgac
     1021 ggcatgccaa gacgcattga ctgatcgccg acgaaaccaa atctcgcggt gcggatagct
     1081 agaccgcgtc gtcgagaccc caatacttgg cttgcctcag aacctcccgc ttgtagctcg
     1141 atctcagcgc gtcgaccata gcgagcgttc gtgatgtcag ttcgtctcga atcccgctct
     1201 gcgcgtgagc gtatgggatg aacgcgcgga gcagatcggg agcgtctagc gcgacgtcca
     1261 atggaacgcg gtcctcgacg tagcgggcgc tgccgatcgc cgaccggaca cgagccgtgc
     1321 tccaccgcaa tgggtcaccg gtgccagtct cgaagagctc cagcagcagc tcccgatggc
     1381 tggagtcggt aaatggcgct gcggaagggg tcgcgaagaa cctgtcacac agctgctcgt
     1441 tcgactccga ttcaacggcc ggcggctggt gttcaccacc ttcgggtagc cgacctacca
     1501 gccactgaac cagtgccctg tagagcggcc atgtatcgga ctcaggtgca agggtcggct
     1561 tgctgagcgc atcctcgatc cacacccgcg cgtcggccag cctcgtctcg acaaccttgg
     1621 tgtcgctgct ggattcggcc acccgtgcaa gggcctcgtc gattggctcc ggtacaacgc
     1681 ctgcgtcggc aacgctcgac aacatgttgt ggtcgatctg gaccgcgatg gtcagctcgt
     1741 gcccaccctc gagtcgcata ccgatcacga gttcgtcaac atccccgaac acattggttc
     1801 tgcgcaccgc ccggtacacc tcgacctgcg gtagcgccgt gatccatcgc ggcagatact
     1861 cacctcgttc ggccagttcg tttcggcatc ggagttgtgg cgctggatcg tcgacgagaa
     1921 gttcggcgat gacggccagt aataccgtcg tctcccgatt acgcacaccg atcaggccgt
     1981 caaggaccgg atgcagataa ttggtatcgc accggccgga cttcaccaat atcggcggtt
     2041 tgggtttcgc cacgtggatc accatgctgg ccaaactgag caggctgagt ggatgcccac
     2101 ccgccagggc accgcgtatt gcgtccctga acaccgtgtc ggcaggaccg ttcacgttgc
     2161 gcttcctcgc ccgccgctcg tcacgtcgga tctgtttggc ctttcgtgct cgtcgttcgt
     2221 gattcatgcc gcggacgcta cttacgggca gtgacaatgc cgcccgccgt tcagagcccc
     2281 ttcgcgtcca gcgccttcat cagcgagggt cgccgaccgt agtcctggcg aatctgcgcg
     2341 atgaacgacg cgaaatcgtc gctgcggccg cctcgttcat agagttcggc catcgtcgcc
     2401 agtctcgcag cgatgtcggg atacagcttg gagttggggt atcgcagatc gttctcgagc
     2461 tgcggccggt acaggtctgc agcctcgaca ggccgaactt cggcgccgcg gtcggccaat
     2521 tcccgccacg cccagccggg accgtaccgg tcggcggctt gccacgcggc atcgatatcg
     2581 ccgtcgctca aatagagctg cactaggacc gcgccggccg cgaagcggtc ggaagccgcc
     2641 agttgtcgcg cgtggtccaa cgcccaggag cgttcggtat cgacgcggtc gacgccggac
     2701 gcgaaatcga gaaggacgcg gtaggcgccc accgacggct gtcggacgaa gtccgcgcga
     2761 aggacggcga ccgcatcgtc gattcggccg agtcccaaat aggtttcggc gacatggtcg
     2821 gggctcagcc aatacgagtt gccaccaccg tggctgctga ttcgtccctc ggcaactgca
     2881 cgatcgatcc acgccactgc atcctcggtg cgtcctgctg cgcgcaaccg gtccacgatc
     2941 gccccgtact gcgggtgttc ccgctgacac agcaagtcca cggcctggtc gacgttgcca
     3001 tcgtgatcag cgagctccag cagcatggcg tcgacttcga accgcttcca gtgatcctga
     3061 ccttcgagct tccggtcgag cgccgccacc gcccggcggt aggtcgcgag cgcatgttcg
     3121 tcgaacgctt ccacgaagtc cgccaagacc aggtgcggcc accccggcga gtcagcgcgg
     3181 aatttcacca gccacgtcgc gagtttgaca gggtcgggct cacccagccg gcaggcccgc
     3241 gcgtagaggt cggcggcccg ctggcattga tcaccgatcg agcccgatga gtcgtcaatg
     3301 tgctcgatca ttttgcgcaa ccgggtgact gcgcgcagca acgctggccg cgcgacttcg
     3361 gcggcgccac ggttcaggtg gttctcgagt tcgtcgagca tttcactggc tactcccgcg
     3421 acctcaaagg aacgtcggta gtcgataaag cctcggaacg ccaacgtgtt tcgtacatag
     3481 gcctccagcg cggctttggc gtgcgcgttg tcacctgccg ccgtcgtggc gcgaatctcc
     3541 agcaggcggc gcactccgtc atcgcgctgc gcaagtgtca ttaccagttc acgcagctca
     3601 tcgacatcca tcgcctgcac gactgcttcc agcgctgcct cggccgcact gccggctgtg
     3661 tcgtcgacgg cgacccgtcc ggtgtcgatc gcggcgaggc cggtggcgac cagatgcttg
     3721 cagaagttgc cgtcggcata atgcgggcag gtacaggatc cgtctggtag cggaccggac
     3781 cagtcgagct cgacttggta gacgttcttg gcttggatcg acgcgtaggc cttgaagtcg
     3841 gtcgtgcgga gaccgcggac ataacgaacg tagtcttcgc cccgcgcgta gaccagatca
     3901 ccggccacct cgagcagtgt cgtttcggag agcggcactt caccatggtg ccggattcgt
     3961 gggccagact cagctgagac ttgggaagca ggcatctcgg cagctgaatt gagaggactt
     4021 gttctccacg gcgtgagcgg cgggacgccg atgagtttct tgcggcgcag ctgtcttatt
     4081 gagggcatag gcaaccgaat ggaccgacgg acggaatgtg acatggcaag aaccaacctc
     4141 tcgatgtcga tctctgccga cggctacgtc gccgggcccg atcaaagcga acagaatccc
     4201 ttaggggtgg gcgggcttgc cgttcacaac tggcatatcg gccccaatac ggaccacccg
     4261 gtcaaccgcc aggtgatgtc cgacatgatg gacggcatgg gcgcaaccat catggggcgc
     4321 aacatgttcg gacctattcg gggcgaatgg gccgactcgg attggcgagg ctggtggggt
     4381 gacactccgc cgtaccactg ccctgtattc gtgcttacgc actacgccca cgatccgatc
     4441 gagatgaacg gcgggacgac gttccatttc gtcaccgacg gcatcgaatc cgcctacgca
     4501 caggctgagg cggctgccgg cggcaaggac atctcgatcg ccggtggcgc atcatgcgcc
     4561 cgccaggcga tcaacgcggg cctggtcgac gagatcgacc tgcaagtggc gccggtgatc
     4621 ctcggctcgg gtgagcgctt gttcgacggc ttccagcccg gcataccgca gctggagctc
     4681 gttcgcgtgc tgcaagcccc cggcgtggct cacctccgct accgtgtcac gcgctgagca
     4741 gatcgggggt aatgagcagc agctatccgc cttcgaggac cgatatggcg tgccgaactc
     4801 gtcgccggag tctcgccaag cgtatcggga acttccgttc ggccgacgag atgggactca
     4861 ataccaacat ctgggctttc ctgttcgggc cgtccgactt cttcccaaaa ggcgtgtggc
     4921 gcaaggacga caacgggaaa ccgaccgact acgaccgagc tgtcaatgtc tgggtcttgg
     4981 taccgatgac ttgcgctctt cgccagacca cttgtcgttc cgggagtgtc atcatctgaa
     5041 tgttgactca ccctacgacg ggttgacgct cacgtactca tcgcgcgaga gaacgagagt
     5101 ttttgtgggc gaagagatat cgacgtaatt atggcctggg ccgtcaactt ctcttattgc
     5161 cgttaggtcg tccgcgattt caaccgcttt ggtgcggtcc agccgccaca cgacacgatc
     5221 ttcgcccatt tcgatatcgg ctgcgccggc ctgcataacg aactcatgca tcactccgcc
     5281 gtgctccagc cgcgacgacc ccagccgcac ggcgtcattc agcgcatccg cgaaagtggc
     5341 cacccccgca ccgtccatgg ccaacaacac cacatcatca ccccacatga aatcggggag
     5401 gagttctacg cggattatgt tcatgcgact ccaagtacac gagcgttggc gatgccgcca
     5461 cgggtataga cgaacggtat caacaacggc ggacatggca ccgacggcga taacgacatt
     5521 tcaccaagaa aattgccgac gggctaccag cgattcccaa attagactcg tcagggtgga
     5581 gtcatcggtc agacttgatc ccgttggtct ggagtggttg gccgcaacgt gcacgtgttt
     5641 ggcggccgag atgacagaca ccgctccaga gctaaccgct ggcccggcgg gtcaggccac
     5701 gtcgcaagcc gtggccgttg tccattccaa tgtcgcgata actcgcgcgc tccttcgtgg
     5761 ccgtttgatc tcgaccgccg ccaaactcac cgcatcagcg agctattacg tgactgcaga
     5821 cgataactcg gcagccgaga tctcagcggt tggtgaaact gtagagacgt agatggctgc
     5881 catatcgcca gtcccatcgc tatcgcagat tcgcgcgtgg gataccgaac atctcgtcga
     5941 tgccgccacc acgtggcgca caacggccga tcactggcac gggtcgttct ctcaagtagc
     6001 cagtcacatg ccggcacccg gcggcacccc atgggaaggc gctggcagtg aaacggcgca
     6061 attgcgtgcg agcaccgacg ccatgaaagt ggacgagata gctgtcagtc tgcgaagcgc
     6121 gtcgacggtc gcgggcaacg gcgccgacga aatcgatggg aaacgccagt cggtgctgaa
     6181 agcaatcgag gatgccgaga gcgcgggttt cagcgtcggc gaggatctct ccgttaccag
     6241 tagatacgcc gacctcacac ccgatgcgga ggttgcgcga caggcgcaag ttgaggcatt
     6301 ggccgcggac atccacgcga aagcaacagc attggctgca gccgaccatg aggtcgcggc
     6361 gaagctcgct tcggccgcgt ccggagttcg cgacgtcacg ttatgcgaga gtccgggtgc
     6421 tgacaccggc gtcgacgcca cgcaacagga gccgcatgta ctcgccgccg gctggaagca
     6481 agggcctagc gacaatcccg cgcctccacc accgtcgcct ccacctcggg tgcggggcct
     6541 gccgcctgaa ggccttcagc cgcctgtctc cgggacgctg acgccgggac cggctagtcg
     6601 ccccagtgag caacgtgtcg gtggccagag tctctgggac gagaagggtg gggaatggcg
     6661 ctactacccg ggcaacaagc atcacaaccc gcattgggac tacaacccgc acgacgttcc
     6721 gaatgcacga tggcagaacg ttccgatcaa caatcttccg cctgtgaagg gcggcccggc
     6781 acctgtcgaa gcagccccgc cgcggcccgc ggtccctgcg tcccctcccg ctgaggcagc
     6841 cccgccccgg cccgcggtcc ctgtgccccc gcccgccgag gcgccaccga tccgcggcgg
     6901 cccaacgggt ggacttcctc tgggcggcgg tgcgctcccg gacggtgcgc tgccgcacct
     6961 cgtcgagcct cccgaaggtg ccgctggtag ccccgacctg ccggtgattg gcgacggcat
     7021 agcagatgtc ccggaggctt gagcgatgac aaaggacggc cggatgacat acgacagtca
     7081 gcgcggccag aacgcctacg agcgcttgcg cgcagaggta ttgactagcg gtttatctga
     7141 ttgggtctcc atcgcggaag tccagcagat catcacccat ttccagctgg ctgaaaccga
     7201 tagtgagcgg caggacctgg tcctcagggc catccgctca ctgctcgaag actgcctaat
     7261 gcagatcggt gagttgcccg ggccggatgg aacgttcccc gcgtgggagc caatcgatgt
     7321 ggcgatggac cgtctgcgcg agcgcttcgt cgaccactac gccgagccga catcgtggga
     7381 ctattcgata tggctgggcc tgaccgaagc aggccgatcg gtcgcggaga aactgcgcgc
     7441 cgggtaacga cggtgagtta cgccgacggt gaatggagct caattcgcgc gcgacgcgat
     7501 cagtctgttc gtcaaagtgg gctaggtgcg gaacttaacc gcggttaatt ggcaaacgtc
     7561 tggatgtcgg tggcatgtgc gaacatgcag gccatgcagc aggattggtt cgccgacctc
     7621 acggggttcc gagaaaccag ctacgacgcc actcgcgagc aacttttcgt tgagggcgac
     7681 gagttggtct cgacggtgag cggcaagcgg tacggcatcg gaacgctcga agtgccgacg
     7741 atggctgaac tgcgtggccg cgtcgaagtt ccgtcggacg ggtgcaccac tgtccggtgt
     7801 gtcgccgggg aggcacggtc catgcatgcc gaccccgaac tcgagggggc gctgttccag
     7861 gtcgcgtcgc agttcaacct gttggagatg acggggccga gcatcacgcc ggaggacggg
     7921 gtgacgcggt acgccgggga tcacacgcag ggtccggcgt gtgcgatcgc cgcgggagcg
     7981 gcgacgatct accgcaacta tttcgcgccc gtagacggtg agaagggcca gacgcgggag
     8041 cgccagatcg acgcgttggc cgcagtaggt gaggcactgt catccaagct cggctggccg
     8101 gtgtctgagc tctggcagat gcgaaacggc tacgcgttgt gcacgacaaa gggtttggac
     8161 ggaatcaccc gcctgctggc ggacggcgac gaggactcgc gcgacgagtt gcgcggccga
     8221 ctcgccattg gacttcaccg caacgtcgaa gtgaccgatg ttcgagaggg cgcgcgacgt
     8281 cttgtctcgc aggcgttctg ctcggcgctg ccagtcgcgt acgggagcgg tccgtcgacg
     8341 gcatgggagg ccttcgcgcg gctggtgctc gaagcggcat atgaggcaac gctgatcgcg
     8401 gcggtcgaac agtcgtcagc gggcgggtcg aacatcgtgc tgctgacccg cgtgggtggt
     8461 ggagcgttcg gcaacgccga cgagtggatc gacgacgcga tcgtccgggc gctggcgatc
     8521 gtcgaacaag cgggactaga catccggctg gtcagccacg gacgcattca cgattcgaat
     8581 cgagcgattg ccgagcggtt caatggatga gaacacgtcg tggccgcatg acgcgatcct
     8641 gcatgcgtgg tgggtcgaac ccggccgtct gctcgctggc gaatatccgg gtgctcgcac
     8701 agcgtcgaaa gcagcggtga aggtccggct tctcatcgac gcgggagtcc actcgatcgt
     8761 cgacctaacg accccggacg accacctcgc ctcgtacgaa gaagcgctac gagacgcggc
     8821 tgagaaggca ggacgccccg tccatcactt cgcctatccc attgccgaca tgggtgtgat
     8881 ccaccgtgag ggctacgacg agatcgtcgc tcgcgtccga ggcgaaatgg actcaggcag
     8941 agtagtttac gttcactgct ggggtggc
//
LOCUS       DASRJA010000071        11149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_783837,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000071 DASRJA010000000
VERSION     DASRJA010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783837"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1011)
                     /locus_tag="VFC01_03245"
     CDS             complement(148..1011)
                     /locus_tag="VFC01_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78694.1"
                     /translation="MSDLGVSDVAFALAVGLCLTLGAVFGSVFYFRRFRLARPAVGVF
                     NGRDVAVMLAFVVVMPLLYVALPTWVLPTMLGLVFFGVLSVSAEPVLRQRWPRRGAIA
                     TLLAIDIVAYQTSTFTIVNSIVIVLVCVGAANLNVQGGFTLRHAVWFVLGLAAYDLVF
                     ITVIPLTQQLFAAAEGYVFAPVAVVRIGDFATAVGMGDILAYALFGLAAYKAYGRVGL
                     RMAVAVVLVFGAIAPPLAPLAIGSLVGHVPQLIPAQVLFAPAAFAAYQVLRRRRGAER
                     RMVSVVSSAVA"
     gene            complement(1008..2441)
                     /locus_tag="VFC01_03250"
     CDS             complement(1008..2441)
                     /locus_tag="VFC01_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN78695.1"
                     /translation="MFETGVRQMRVAFATVFGRPIPVRALQRLLADALATLEEFGSPG
                     DDVQHLLDGPYADPGLRVQLQVKALRRTARRMTARSSFYRDRFAASDVDPARLTLDTM
                     RAVPVTARWELDAHGPAMRCGRPFLSTRTSGTTAAPLEVWLSRYEMELWPALVALSMV
                     LRGELGPNDHVQISTNVRATATMQADVELCRLVGAACSVVGLVDPEEGLDRLRGRFGL
                     APTVLNTYPSYLGALVTAARDRGLGPDDFALHTVNVGGEMMTVALADAARQTLGASRI
                     DDSYGATELMPFGGRSCEQGHLHLDPNLGYAEVLDLSTMEPVEPGELGTLVVTPYFPY
                     RECTPVFRYDTRDLVRLPVAQRTTCELAAIPAVSQILGKANDLVHTADGPVTPRQIAE
                     VLEAIPGVRWPVRFTAHATGGRLRITVPRSCLPPEVNRPGLAETCAARGIDAEVVVAV
                     GDPPGGRPLRADFVLPRLGRQPSVAVS"
     gene            complement(2794..3492)
                     /locus_tag="VFC01_03255"
     CDS             complement(2794..3492)
                     /locus_tag="VFC01_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78696.1"
                     /translation="MRYARSMVPVALTAALLAGCGTTPATPSSSPSPAKSAALSAKEI
                     VQKSGEALNKAGSYRMRGDIVSSGDKMSIDVKVQGSESFAVISMGDQGSITVLNVAGT
                     SYFQADEVFWKKTGGIDAKTYNSVFKDKWVKPKAGDTNMQKFTEFTDAKAFLDPAGSE
                     SLTVGEKTTINGTSALTLKDPSASGSALYVAAEGEPYPLRVEHGTDGKIDFSEFGAKF
                     AEVKAPPPDKVISL"
     gene            complement(3724..4308)
                     /locus_tag="VFC01_03260"
     CDS             complement(3724..4308)
                     /locus_tag="VFC01_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZN78697.1"
                     /translation="MPTTYTFDVFSSLDGYGSVSESANWGGYWGKQGPELLDHRLALY
                     GRQQRMVFGANTYRAFAQMLAASTEESDVRDPWVTRMRNLPATVVSTTLQGPLDWPDA
                     TVVSGDAVDVVARLKEESEMPLRSHGSLSLNRALMAAGLVDFVQVTIFPVITGQTGQE
                     PIFQGAADFDLQLIEHRTLDGRTQELIYRPTLHV"
     gene            4563..5336
                     /locus_tag="VFC01_03265"
     CDS             4563..5336
                     /locus_tag="VFC01_03265"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN78698.1"
                     /translation="MTINEETYGLTGAAFYDAYFPVDARALETATFLARCARESGTDE
                     PIALELGIGTGRVAFPLADQGVKVFGVDLAPAMLQILRDKPEAHRLIVALGDMTDAAT
                     LASISGGVGYDLVYCVFSTLTALPDAAAQRRCLAAAASMLAPNGRLVLEMPVPDLTTF
                     DKNRRRVRHLGRYGDGTTMETARLDPVAQTINFEVTVLSPTTGVTLQPVRSRYVWPHE
                     LNLMAEFAGLRPIARYGNWTHGPFGSDSAINVTVYSGRS"
     gene            5760..6107
                     /locus_tag="VFC01_03270"
     CDS             5760..6107
                     /locus_tag="VFC01_03270"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZN78699.1"
                     /translation="MKYIMLIYQGAALERQAALSEEEQKQVYADYQGINQTPGVTPGL
                     PMGLPENATTVRVEGGKTLTTDGPFVGMKEAVGGWFILEADDLDAAIEVASRVPAARY
                     GGAVEIRPSEVYW"
     gene            6101..7288
                     /locus_tag="VFC01_03275"
     CDS             6101..7288
                     /locus_tag="VFC01_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN78700.1"
                     /translation="MVTTLEQIFREEWGRVLAVLVGFFGDFDLAEEAAQEAFAIAADR
                     WPRDGIPSNPRAWLMTTARNRATDRIRRDRALVARFGLLVADDRAEVPMNATIFPDER
                     LELIFTCCHPALAVDAQVALTLRTLGGLTTDEIARAFLVPERTMAQRLVRAKRKIKAA
                     GIPFRVPPVHLLRERLDAVLAVVYLIFNEGYGGRDELAAEAIWLARALAELLPDDPEV
                     HGLLALMLLHDSRRETRFSDGELVLLGDQNRSRWNTEQIAAGRAELDRALALGGRGPY
                     VLQAAIASLHAETPCDWAQIAALYGQLARLTGSPVVELNRAIAIAETEGPEAGLRIVD
                     ELGLHDFRYLHSTRAELLRRLGRTDEARDAYRRARQLTDDGAERRFLERRLTELAGMT
                     DSG"
     gene            7541..10225
                     /locus_tag="VFC01_03280"
     CDS             7541..10225
                     /locus_tag="VFC01_03280"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN78701.1"
                     /translation="MGWLLQEAGAAARRRFSDQVVRVLTGVVDAAVSQAVARVYVDAE
                     QREHTIALLKERPASALPLVDGAPLGQLATAVGLWVASIEAPVDDDGPPPPVTNHPLA
                     RALCREILAGIEHEALRGEGVLYPLWSDRQRTIDTKKILASIEDVRRSVSEPSSGRGG
                     RSGSRPSRIAYWHPPVAHPSIFVGRDAELTWLSMPDGGRMRLVYGVGGVGKTAVVLAF
                     AEKVGDAFPDGRVFVDFQSYSEHPRHHRKTAEQALAELLPICGIDDQLIVGTDLQQRE
                     LLWKQAISGRRMLFFWDNVGSLAQIEPLLTGQPGCLTLITSRSELDVPADSLKLEHLS
                     EQDAIELFAVVAGGESNCLGQELVSDAVRLCAWMPLQIMVHAASVRRRRSLPELVTEL
                     AKLPTSDRLARLFASLDLSYQELTEDVQRAWRVLGTHPGPHLTAGTAAAMLGCAVEEA
                     VKLLDELVDANFAERHRDRFDLYGGGHEIASEPRFYAYVAHDLLRDYAVHRAARSIDE
                     MLVHVKRLLDHYWERLTADIDHAWIQVERACIVLALWLVKPNEDIRDFAMQAGGRLRE
                     AGWYVDAEVVDWYALHLSREARARDGEAYALWSLAQAAVTRGDHEQAVEHLHAALTIN
                     REIGNRQGEAEVLMDLGMSAIFRGRHQEGFAALEDALALSRELGDRNLEFSVLLQLAQ
                     QRLALSAMTRTDLDRAARQARALLRLCHKLDERRRASALLFLAQVKEIQRATKAAAEA
                     YQSALEIFLVIGHRHGQANALFGIGSVMLQRGDEAQAAARWRKALAIYREVSDRQGEA
                     KTLAHMAEVAISRGALDEAKQLLLDAHTTYQQSGIPLGQAAIAKRLAEVASALGQQSQ
                     AHAHLRESLSLLETINSPHADKIRQQFNDLTRPSATQE"
     gene            10492..11040
                     /locus_tag="VFC01_03285"
     CDS             10492..11040
                     /locus_tag="VFC01_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZN78702.1"
                     /translation="MRLLRYSINVTLDGCCDHRAIPADEDLHRHAVENLNQADALLFG
                     RVTYEMMESAWRPAAPAGARPDWMEPFAQTINAAKKYVVSSTLERVDWNAELVRGDLG
                     KAVQQLKRESGKGLFTGGVKLPLALAELGLIDEYEFVVHPRLAGHGPTLFAGLSKHID
                     LKLLSRLEFGSGAVAMRYEPRR"
BASE COUNT         2027 a         3579 c         3620 g         1923 t
ORIGIN      
        1 caggctgctc gggcgggtgt ggcgtgagcg ggacgagtat cggcgcgccg acggttgccg
       61 ccagcgcgcc gatgccatcc tcgccggact gcctcccctt ggcggcccgg tagcccccga
      121 tgacccgtgg ccgccggtgc ccgcggatca ggccacggct gatgaaacca ccgacaccat
      181 tcgccgttcc gcaccacggc ggcggcgtag cacttgatag gcggcgaagg ccgccggcgc
      241 gaacagcacc tgcgccggga tgagctgagg gacatggccc accagcgatc cgatggcaag
      301 tggcgcaagc ggcggcgcta tcgccccgaa gacgagcacc acggcgacgg ccattcgcag
      361 cccgactcgc ccgtaagcct tgtaggcggc cagcccgaac aacgcgtacg ccagaatgtc
      421 gcccatgccc accgcggtcg cgaaatcacc tatccgcacc acagccaccg gcgcgaagac
      481 gtaaccttcg gcggccgcga aaagctgctg tgtcaacgga atcacagtga tgaaaaccag
      541 atcgtaggcg gccagcccaa gcacgaacca gacagcgtgc cgcagggtaa acccgccctg
      601 cacgttgagg ttggccgcgc cgacgcagac cagcacgatc acgatgctgt tgacgatcgt
      661 gaacgtgctg gtctggtacg cgacgatgtc tatggcaagc agcgtcgcga tcgcaccgcg
      721 gcgtggccaa cgctgccgca gcaccggttc ggcgctgacc gacaggacac cgaagaacac
      781 cagcccgagc atcgtgggta ggacccaggt cggcagggcc acgtacaaca gcggcatgac
      841 gacaacgaat gccagcatca ccgcgacatc gcgcccgttg aagaccccga ccgcggggcg
      901 ggccaggcgg aaacgccgga agtaaaacac cgagccgaag acggcgccca gcgtcaggca
      961 taggcccacc gcgagggcga aggcgacatc actcacgcca aggtcgctca tgacaccgcc
     1021 accgagggtt gccggccgag gcgcggcagc acgaagtcgg cgcgtagtgg gcgtccgccc
     1081 ggcggatcgc caacggcgac aacaacttcg gcgtcgatgc cgcgagccgc gcaggtctcg
     1141 gccaggccgg gtctgttcac ctccggcggc aggcagctgc gcggcaccgt gatgcgcaga
     1201 cggccaccgg tagcgtgggc ggtgaagcgc accggccagc gcacacccgg gatcgcctcg
     1261 agaacctcag cgatctgccg cggggtcacc ggcccgtccg ctgtgtgcac caagtcgttg
     1321 gccttgccca gaatctgcga caccgcggga atcgccgcca gctcgcaggt ggtccgctgc
     1381 gccaccggca accggaccag atcgcgggtg tcgtaacgga acaccggcgt gcattcgcgg
     1441 tacgggaaat acggtgtcac gacaagcgtt cccagctcac ccggctcgac aggctccatt
     1501 gtggacagat caagcacttc ggcgtacccg aggttcggat ccaagtgcag gtggccctgt
     1561 tcgcaggacc gtccgccaaa gggcatcagc tctgtcgcgc catacgagtc gtcgatccgg
     1621 ctggcgccaa gcgtctgccg cgcagcgtct gccagcgcga cggtcatcat ctcgccgccc
     1681 acgttgacgg tgtgcaacgc gaagtcgtcc gggccgaggc cgcggtcgcg agccgcggta
     1741 accagcgcgc ccaggtagct cgggtatgtg ttgaggacag tgggggccag gccaaaccga
     1801 ccacgcagcc ggtcaagccc ctcctccgga tccaccagcc ccaccacgct acacgccgcc
     1861 ccgaccaacc ggcacagctc gacatcggcc tgcatcgtgg cggtcgcgcg cacgttcgtg
     1921 gaaatttgga cgtgatcgtt gggcccaagt tcgccgcgca gcaccatcga cagcgccacc
     1981 agcgccggcc acaactccat ctcgtacctt gacagccaga cctccagcgg cgcggcggtg
     2041 gtgccgctgg tgcgggttga caggaacggc cggccgcaac gcatcgccgg cccgtgggca
     2101 tccagttccc aacgggcggt gaccggcacc gcccgcatcg tgtcgagggt gagccgagcc
     2161 ggatccacat cagacgcggc gaaacgatcc cggtagaacg atgaccgcgc cgtcatccgc
     2221 cgcgccgtgc gacgcagcgc cttcacctgc agttgtaccc gcaggccagg gtccgcgtaa
     2281 ggaccgtcca acagatgttg cacatcgtcg cccggcgaac cgaactcctc cagcgtcgcg
     2341 agcgcgtcgg ccaacaaacg ctgcagcgcc cgtaccggga tgggacgccc gaacacagtc
     2401 gcgaaggcca cgcgcatctg gcggactccg gtttcgaaca cgtgtcactc ctcaggcagg
     2461 agggtggtgc cgggtggcag ccgccacccg gcaccggtgg ttgttcggtt ctagtgccga
     2521 aggcagttca ggaagaacac gcctgccatc gcacccatga catacttggt gtgcttcact
     2581 gtgctgatga tcgcggccat cgtcgcctcc ttcccctctt ccagttggcg gatagatcga
     2641 cgctaaggcg agcaccgtat aggagctgga tatctggcgt atacgacggg ataaccgcag
     2701 cgcatgaccc ggtttgtctg gctcggttct cggcgtgtgg cggggcgcat cccgtcctgg
     2761 gccgatcatc gccgcgctat ggcactcctg tcattaaagg ctgatcacct tgtcgggcgg
     2821 cggcgccttg acctcggcga acttcgcgcc gaactcactg aaatcgatct tgccatcggt
     2881 gccgtgttcc acccgcagcg gataaggctc accctccgcg gcgacgtaca acgcgcttcc
     2941 cgaggcggaa gggtccttga gcgtcaacgc cgacgtgccg ttgatagtgg tcttctcgcc
     3001 cacggtcaac gactctgagc ccgcgggatc caggaacgcc ttcgcgtcgg tgaactcggt
     3061 gaacttctgc atattggtat cgcccgcctt gggctttacc cacttgtcct tgaaaacaga
     3121 gttgtaggtc ttggcgtcga tgccaccggt cttcttccag aagacctcat cggcctggaa
     3181 gtaggacgtc ccggcgacat tcagcacggt gatggaacct tgatccccca tggaaatgac
     3241 ggcgaaggac tcggaaccct gcaccttgac gtcgatggac atcttgtcgc cgctactgac
     3301 gatgtcgccc ctcatgcggt aggatccggc cttgttcaag gcctcccccg acttttggac
     3361 gatttccttc gccgagaggg cggcgctctt ggcgggtgac gggctggatg acggcgtggc
     3421 cggcgtggtg ccacaaccgg caagcaaagc cgcggtcaga gcgaccggga ccatggagcg
     3481 cgcgtagcgc acgatgttct ccttcgggtt ggaggccggt cctcgcctgg acggccaatg
     3541 gaccgcgagc ataaggccgg ccgtggctct cgcgctgtcc cgtgcgctga ggtgtacgcc
     3601 gacgggtaca tgccttacct tcgccgcctc gccgtctttg atcagcggtt cgtccgctcg
     3661 gccacaccgg tttcattcac caccctgccg ccgagcagta ccccggcgtc gcaagggacg
     3721 gactcagaca tgcagggtgg gccggtagat gagctcttgg gtgcgaccgt cgagcgtccg
     3781 gtgctcgatc agctgcaggt cgaagtcggc cgccccctgg aagatcggtt cctgcccggt
     3841 ctgaccggtg atcacaggga agatcgtcac ctgcacgaag tcgaccaggc ctgcggccat
     3901 cagcgcccgg ttcaacgaca ggctgccgtg cgagcgcaac ggcatctcgg attcctcctt
     3961 gagccgggcg acgacatcga cggcgtcacc gctcacgacg gtcgcgtccg gccagtcgag
     4021 gggtccttgc agtgtcgtcg acaccaccgt tgccggtagg ttccgcatcc gggtgaccca
     4081 ggggtcacgc acgtcggact cctcggtgct cgcggccagc atctgcgcga acgcgcgata
     4141 tgtgttggcc ccgaagacca tccgctgctg ccggccgtac aaggcaaggc ggtggtcgag
     4201 cagttcgggt ccctgcttac cccagtagcc gccccagttg gcgctttcgc tgacggagcc
     4261 gtagccgtcg aggctggaaa agacgtcgaa ggtgtaagta gtgggcatga tgctctccta
     4321 gtgaacggtt gatcgggttt gagagtaaga ctggaaccca cggccaaact catcgtcgtt
     4381 ggccgttacc ggcatcctcg gcccggacaa cgacacaacg ctcgtctggg tttcaccgcg
     4441 aatccgcggc cttgcccgag cgtgggcgcg caatgttggt gtctatatct cgaatcgcgc
     4501 ggcgcaggcg gccgtacccg cccatttgtg tggctacggt tcgccgtgcg aaggtcggca
     4561 tcgtgaccat caatgaagag acgtacggcc tgaccggtgc cgcgttctat gacgcatact
     4621 tcccggtcga tgcgcgcgcg cttgaaactg ctactttcct ggctcggtgt gctcgggaaa
     4681 gcgggacgga cgagccgatc gcgctcgagc tcggcatcgg caccggccgg gtcgcgtttc
     4741 cgctggccga tcagggcgtc aaggtgttcg gcgtcgatct ggctccagcc atgctgcaga
     4801 tcctgcggga caagcctgag gcacatcgcc taattgtggc gctcggggac atgaccgacg
     4861 cggcgacgct cgcctccata tccggcgggg ttggctacga ccttgtctac tgcgtcttta
     4921 gtaccctcac cgccctgccc gatgccgcgg cgcagcggcg atgcctcgca gcagccgcgt
     4981 cgatgctggc cccgaacggg cggctcgtgc tcgaaatgcc ggtaccggac ctgaccacgt
     5041 tcgacaagaa ccgacgccgt gtgcggcacc tgggccggta cggcgacggc acgaccatgg
     5101 agacggcccg cctggacccg gtcgcccaga cgatcaattt cgaggtgacg gtgctttcgc
     5161 ccacgacagg tgtgaccctc caacccgtac gcagccgata tgtatggcca catgagctaa
     5221 acctgatggc cgagttcgct ggactgcggc cgattgcccg ctacggcaat tggacgcacg
     5281 ggcccttcgg cagcgattcg gcgatcaacg tcaccgtcta ctctggacgc tcttaagcca
     5341 cggcacgacg aaagctcctc gatacggttt agcacataag gcttgtccgc ctatgcgccc
     5401 ggatacgact cggcggcaag gcataggtcc gccgcgtggg cgccgaacct caaaccgcgg
     5461 gcgataccgg ttccgggccg gtttccgttg caccgagcca gaacgccgac cggcagcacc
     5521 atcgctatca gcgcgagacc gtccgcatcc gcgtggaggg ctcgggcaag gggccgcaca
     5581 gttggcttcg cctcacccgg gcgaccgtgc gacccgccgg aaggaccgtc gccgcccatc
     5641 gcgccggagc cgacggccga gtcagcgggg ggtcaaagat ttctggtgtc gagctgtcga
     5701 tttcctggct ggtcgttcgt cttagtgggt gactatctgg tagtgacgag gaggacgcga
     5761 tgaagtacat catgctgatc tatcagggcg ccgcgcttga gcgacaggcg gcgctgtctg
     5821 aggaggagca gaagcaggtc tacgccgact atcaagggat caatcagacg ccgggtgtca
     5881 ccccggggct gccgatgggc cttcccgaga acgcgaccac cgtgcgggtc gaaggcggca
     5941 agaccctgac aaccgacggc ccgttcgtcg ggatgaaaga ggccgtcggc ggttggttca
     6001 tcctcgaggc cgacgacctc gacgcggcca tcgaggtggc ctcccgcgtc ccggcagcgc
     6061 gctatggtgg cgccgtcgag atccgccctt cggaggtgta ttggtaacga cgctcgaaca
     6121 gatcttccgc gaggagtggg gccgcgttct ggccgtcctg gtcggcttct tcggcgattt
     6181 tgacctcgcc gaagaagccg cgcaggaggc gttcgccatc gccgccgacc ggtggccacg
     6241 cgacggcatc cccagcaacc cgcgtgcctg gctgatgacg acggctcgga accgagcgac
     6301 ggatcgcatt cgccgcgatc gggctctcgt ggccaggttc ggcctgctcg tcgccgacga
     6361 tcgtgctgag gtgcctatga acgccacgat ttttcccgac gagcgtctgg agctcatctt
     6421 cacctgctgt cacccggcgc tcgcggtcga tgcgcaggtt gcgctcacct tgcgcacctt
     6481 gggcggactg accaccgacg agatcgcccg tgccttcctg gtaccggagc gcaccatggc
     6541 gcagcggctg gtgcgggcca aacgcaagat caaagcagcc gggatcccgt ttcgggtacc
     6601 accggttcac ctgctccgcg agcgtctcga cgccgttctc gccgtcgtct atctgatctt
     6661 caacgagggc tacggcggac gcgacgagtt ggcggccgag gcgatctggt tggcgcgcgc
     6721 actcgccgag ctacttcccg acgaccccga ggttcacggc ctcctggcgc tgatgctcct
     6781 gcacgattcc cgacgcgaga cgcggtttag cgacggcgag ctcgtgctgc tcggcgatca
     6841 gaaccggtcc cggtggaaca cagagcagat cgccgccggc cgcgccgagc tcgaccgggc
     6901 gctggccctg ggcggtcgcg gtccgtacgt gctgcaggcc gccatcgcct cgttgcacgc
     6961 cgagacgccg tgcgactggg cgcagatcgc ggcactgtac ggccaactcg cccgcttgac
     7021 cggctcgccc gtcgtggaac tcaaccgcgc catcgccatc gccgagaccg aaggcccgga
     7081 ggccggcctg cgcatcgtcg acgaactcgg tctccacgac ttccgctacc tgcattccac
     7141 ccgggccgaa ctcctgcgac gcctcgggcg taccgatgag gcgcgtgacg cctaccgacg
     7201 tgccagacaa ctcaccgacg acggcgctga acggcgcttc ctcgaacgcc ggctcacgga
     7261 actggcaggc atgacagact ccggttaagc cacggttaaa gcctggattc acagaagccc
     7321 ggcctaccct gattcggggt tgagctgggc ttctgtttcg catgtccgcg caggtcaggg
     7381 ctatggctcg aacgccgtga ccaggtaccg gctcgggaac atagggcttt cgcgagacat
     7441 cgacaaagcg gctgatcatg gactgcacct cgtatggtga gagatacatc gacgatgtgc
     7501 cataggagtc gttttggtgg aaaccttcat aggtgccctc gtgggctggc tgcttcagga
     7561 ggcaggcgcg gcggcgcgcc gacggttcag tgatcaagtg gttcgggtgc tgaccggagt
     7621 ggtcgatgcg gcggtatcgc aagcggtggc tcgggtctat gtggatgccg agcaacgcga
     7681 gcacacgatc gccctcctca aggaacgccc ggccagcgcg ttaccgctgg ttgatggagc
     7741 acctttgggt cagctggcga cagccgtggg cctttgggtg gcaagcatag aggcgccagt
     7801 cgatgatgac ggtccgccac cgccggtcac gaaccatccg ttggcacgag cgttgtgccg
     7861 cgagatcctc gccgggatcg aacatgaagc gctacgcggc gagggcgtgc tgtatccgct
     7921 ttggagcgac cgtcaacgca cgatcgacac caagaagatc cttgcaagca ttgaggatgt
     7981 ccgacggtcg gtgtccgagc cgtccagtgg tcgtggtgga cggagcggta gtcgaccatc
     8041 cagaatagcc tattggcacc cgccggtcgc tcatccgagc atattcgtgg gccgcgatgc
     8101 tgagttaact tggttatcca tgccagacgg cggccggatg aggctggtgt atggcgtcgg
     8161 cggagtcggc aagaccgctg tggttctggc gttcgctgag aaagttggcg atgccttccc
     8221 ggacggccga gtgtttgtgg attttcagag ctattctgag cacccgcgac accaccgcaa
     8281 gaccgccgaa caggctctgg ctgagctgct tcccatttgt ggaatcgacg accagctgat
     8341 agtcggtacg gatctgcagc agcgtgaatt gctgtggaag caggcgatct cggggcgccg
     8401 gatgctgttc ttttgggaca atgtgggcag tctggcgcag atcgagccgc tgttgaccgg
     8461 ccaaccgggc tgcctaactc tgatcacaag ccgttcggag ctggacgtgc cggctgattc
     8521 gctgaagctt gagcatctgt ccgagcaaga tgcgattgag ctcttcgctg tcgtcgccgg
     8581 tggagagtct aattgcctgg gtcaggagct ggtatccgac gcggtgcggc tttgtgcgtg
     8641 gatgccgctg cagatcatgg tgcatgcagc aagtgtccga cgtcgacgca gtctgcctga
     8701 actcgtaacg gagttggcca agctgccgac aagtgaccga ctcgctcgtc ttttcgccag
     8761 cctcgacttg tcctaccagg aactcaccga ggacgtccag cgggcatggc gtgtacttgg
     8821 cacgcatccc ggcccacatc tgacggccgg gacggccgct gccatgttgg gctgtgctgt
     8881 cgaggaagca gtaaagcttc tcgacgagct cgtcgacgcc aacttcgccg aaaggcaccg
     8941 tgaccggttc gacctttacg gtggcggcca cgagatcgcc agcgagcccc gcttttacgc
     9001 gtatgtagca cacgatctgc tgcgcgacta cgcagtccat cgggcagcac ggtctatcga
     9061 cgaaatgctt gtgcacgtca agcggctgct cgaccactac tgggaacgac tcacagcaga
     9121 tatcgatcac gcctggatcc aagtggaacg cgcctgcata gtgttagccc tatggctcgt
     9181 caagccgaac gaggacattc gcgacttcgc catgcaggcc ggtggtcgcc tgcgggaagc
     9241 cggctggtat gtcgacgccg aagtggtcga ctggtatgca ctgcacctgt ctagggaagc
     9301 gcgagcacgg gacggcgaag cgtacgcgtt gtggagcttg gctcaggctg ccgtgactcg
     9361 cggtgatcac gaacaggcgg tggagcacct gcacgctgcc ctcacgatta atcgggagat
     9421 cggcaaccgg caaggggagg cggaggtcct gatggacctc ggcatgtcgg cgatctttcg
     9481 aggccgtcat caggagggat tcgccgcgct ggaagacgcg cttgcgctca gccgtgaact
     9541 tggcgaccgc aaccttgagt tcagcgtact tttgcagctg gctcagcaaa ggctcgcgct
     9601 gagcgccatg acacggactg acctggaccg cgctgccagg caggcccgcg ctctcctccg
     9661 gctctgccac aagctagacg agcgcaggcg tgcatcggct ctcttgttcc tcgcccaggt
     9721 taaagagatt cagcgcgcga ccaaggcagc ggcggaggcg taccaatctg ctctggagat
     9781 ctttctggtc attggccatc gtcacggaca ggccaacgct ctttttggaa tcggctcggt
     9841 catgctgcaa cgcggtgatg aggcgcaagc agcggcccgc tggcggaagg cactggcgat
     9901 ctatcgggaa gtcagtgatc gccaaggcga ggccaagaca ttggcgcata tggctgaagt
     9961 ggcgatctcg cgtggtgcgc tcgacgaagc gaaacagctg ctgcttgatg cgcacacgac
    10021 gtaccaacag tccggaatcc cgctggggca ggcggcaatc gcgaagaggc tggcagaggt
    10081 ggcctcagca ttgggacagc agtcccaggc gcatgcccac cttcgagaaa gcctcagcct
    10141 cctcgaaacg atcaactcgc cacacgccga caagatccgg caacagttca acgacctaac
    10201 aagaccatca gcaacgcaag aatgacatgg agcccgccga cttcgagttt tggacaaagt
    10261 cagatggccg aaccgcgagc gaggacaagc ggacaatttc gcgcgagcgg cgcttcgccc
    10321 gtgatcgcga ggtgacgggt ccgccggtcg atggcttcga gatcagctac gtggccggtg
    10381 atcggcacca ctgccgcccg cgccgaaaca cggacgcttc gccggtcaaa attcctcata
    10441 caaatcggcc gcctcaacac gagaaagatc aattggcggt acccttgcga aatgcgtctc
    10501 cttcggtact ccatcaacgt cacattggac gggtgctgcg atcatcgtgc catccccgcg
    10561 gacgaagact tgcatcgtca cgcggtcgag aacctcaacc aggccgatgc ccttctcttt
    10621 ggccgggtga cttacgaaat gatggaatcg gcgtggcggc cggcggcgcc ggcgggagcc
    10681 agacctgatt ggatggaacc cttcgcccaa acgatcaacg cggcaaagaa gtacgtcgtg
    10741 tcgagcaccc tggagcgggt cgattggaac gcggagctcg tgcgcgggga tctgggcaag
    10801 gccgttcagc agctcaagcg ggagtcgggc aagggcctgt tcacgggagg cgtgaagctc
    10861 ccgctggcgt tggcggagct gggattgatc gatgagtacg agttcgtggt gcatcccagg
    10921 ctggcgggcc acgggccaac gttgttcgcg gggctatcga agcacatcga cttgaagctc
    10981 ttgagccggc tggagttcgg ctcgggggcg gtggcgatgc ggtatgagcc gagacggtag
    11041 ccactcgctc tttacggtcg ccagacgtga cttggccgtg ttggcatgca acttattcga
    11101 gtcagaaccg attcaaagga cctagtcctg ttccagcaag ttccggaac
//
LOCUS       DASRJA010000072         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3322078,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000072 DASRJA010000000
VERSION     DASRJA010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3322078"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VFC01_03290"
     CDS             <1..1540
                     /locus_tag="VFC01_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78703.1"
                     /translation="RSKGRCAVGTNGRGGDALADLAAAVGEADRGGSLYETANALARV
                     VVERADADEALRMLSAVRPRTWLRLDVELRSLRHFFHPRGEWQQITAAAWAGANPLAL
                     LLTACSGDGRLRQRAVHMPLMRSDQRLLPVLLIRTADWAKQVRDDARQVLPTALDAAN
                     TDGLIRAVGVALAMREWQRGEGAFAAVTEALRTRSDGTLDAARTSDDLQVRRLAYRVW
                     LESGHADSDALVAAALTEPDIICQSLSVDGVVRAAVRDRQRDALDRLLAARFARVRVE
                     ALAGLVQIGHPEAGEAFLADRSAMLRATAQWATRRAGRDAAERYRAMLASGDDSLLRA
                     AVAGLGECGAVDDAELVAGFLWHDRPRVRAEAVRAVRRLGGALNQIARMLTDPAPVVV
                     RAVADAVLGQPGLVPIDRLWELVAADQPAHVRRTVFRLLIARDTWTRIEADLRLVGDS
                     DDRLRPHARSNLTSWLDHEASTAYQMPPRTTRERLGRLIDAAEPNIGAPKAHLLRWHL
                     ALSR"
     gene            complement(1537..>2368)
                     /locus_tag="VFC01_03295"
     CDS             complement(1537..>2368)
                     /locus_tag="VFC01_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZN78704.1"
                     /translation="SWPDSIRDRLTTLAGVLDDTVVDTLWVADHLLQADPASQLDEPM
                     LEAYTTLGYLAAATSRIRLGTMVTAATFRAPALLIKAVTTLDVLSAGRAWLGIGAGYN
                     SDEARAMGLFLPETAERFTRMTELLRLARQMWNGDETPFRGEYLDLEHPVGSPCPVTT
                     PPVLIGGTGERRTLRLVAEYGDACNLFDVPDGGTAIRRQLSVLDRHCADVGRPPEEIE
                     RTVTTALQDGDSSDQLAQRCRKLGDLGVRHVVVIARGRPLTDTDLNCVAGVADQLAAA
                     "
BASE COUNT          353 a          783 c          854 g          378 t
ORIGIN      
        1 ccgcagcaag gggaggtgtg cggtggggac gaacggcagg ggtggcgacg cgctcgcgga
       61 tttggctgcc gccgttggag aagcggatcg cggaggcagc ctttacgaga ccgcgaatgc
      121 cctcgcacgg gtggttgtcg aacgggcaga cgccgacgaa gcactgcgga tgctgtcggc
      181 cgtccgcccg cgcacctggc tgcggctgga cgtcgagcta cggagcttgc gccatttctt
      241 ccaccctcgc ggcgagtggc agcagatcac cgccgctgcc tgggccggcg cgaatccact
      301 cgccttgctc ctcaccgcat gctcgggcga cgggcggctg aggcagcgtg cggtgcatat
      361 gccgctgatg cgcagtgatc agcggttgct ccccgtgctg ctgatccgga ccgccgactg
      421 ggcgaagcag gtgcgggacg acgctcggca agtgctcccg accgcgctcg acgcggccaa
      481 tacggacggg ctgataaggg cggtcggcgt ggcgctggcg atgagggagt ggcagcgcgg
      541 cgaaggagcg ttcgcagcgg tgaccgaggc actacgcacg cgatcggacg ggacgcttga
      601 cgcggcccgc acgagcgacg accttcaggt gcgccggctc gcttatcggg tgtggctgga
      661 gtcgggccac gccgactcgg acgcgctggt tgcggccgcc ctgaccgaac ccgacatcat
      721 ttgtcagagc ctgagcgtcg atggtgtcgt ccgcgccgcc gtgcgcgacc ggcagcgcga
      781 cgcgttggat cgtttgcttg cagcccgttt cgcccgcgtg cgcgtcgagg cgcttgccgg
      841 actggtccag atcgggcacc cagaggcggg cgaagcgttc ctagccgatc ggtcggcaat
      901 gctgcgagcc accgcgcaat gggcgacgcg acgagcaggc cgtgacgcag ccgagcgata
      961 ccgagccatg ctggcgtcgg gtgacgactc actgctgcgc gcagccgtgg cggggctcgg
     1021 cgaatgcggc gccgtcgacg acgcggagtt ggtcgcaggt tttctctggc acgaccgtcc
     1081 gcgggtgcgc gcggaggcgg tgcgggccgt gcgccggctg ggcggcgcgc tcaaccagat
     1141 cgcgcggatg ctcaccgacc cagctcctgt ggtcgtgcga gcggtcgccg acgcggtgct
     1201 tggccagccc ggtcttgtgc ccatcgatcg gctgtgggag ctggtggccg cggatcagcc
     1261 agcacatgta cggcgcaccg tgttcagact gctgatcgcc cgtgacacgt ggacacggat
     1321 cgaggcagac ctccggctcg tcggcgactc cgacgaccgg ctccggcctc acgccagatc
     1381 gaatctgacc agctggctcg accacgaggc ctcgacggcg taccagatgc caccccggac
     1441 cacccgcgag cgcctgggac ggctcatcga cgccgccgaa ccgaacattg gtgccccgaa
     1501 ggcccaccta ctccgctggc acctcgccct ctcgcgttaa gcggcggcga gctgatccgc
     1561 gactcccgca acacaattga gatcggtgtc ggtcagcggc ctgccgcggg cgatcaccac
     1621 gacgtgtcgc acgccgagat cgcccagctt ccggcatcgt tgcgccagct ggtccgagct
     1681 gtcgccgtct tggagcgccg tggttaccgt gcgctcgatc tcctcgggcg gccggccaac
     1741 gtcagcgcaa tgacgatcga ggacgctgag ctgcctgcgg atcgcggtac cgccgtcggg
     1801 cacgtcgaac aggttgcatg cgtcgccgta ctcggcgacc agccgcagcg tccggcgttc
     1861 gccggtgcct ccgatcagca ctggaggcgt tgtgacgggg cacgggctgc caactgggtg
     1921 ctcgaggtcg agatattcgc cgcggaaagg tgtttcgtcg ccgttccaca tttgcctggc
     1981 cagccgcagc aattcggtca ttcgggtgaa ccgctcggct gtctcgggga ggaacagtcc
     2041 catcgcgcgt gcctcgtcgc tgttgtagcc cgcgccgatc cctagccacg cccggccggc
     2101 ggacagcacg tcgagcgtag tgacggcctt gatcagcaac gccggtgcgc ggaatgttgc
     2161 agcagtgacc atggtgccca agcggattcg cgacgtggcg gccgcgagat atcccagcgt
     2221 ggtgtaggcc tccagcatcg gttcgtcgag ctggcttgcg ggatcggctt gcagaagatg
     2281 gtcggccacc cacagtgtgt cgacgacggt atcgtcgagc acgccggcga gtgtcgtgag
     2341 ccggtcgcgg atcgagtcgg gccacgag
//
LOCUS       DASRJA010000073         8767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_504944,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000073 DASRJA010000000
VERSION     DASRJA010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504944"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFC01_03300"
     CDS             complement(<1..474)
                     /locus_tag="VFC01_03300"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZN78705.1"
                     /translation="MSIAESSIPIAVPVPTGGDDPTKVAMLGLTFDDVLLLPAASDVV
                     PANADTSSQLTKRIRLKVPLVSSAMDTVTESRMAIAMARAGGMGVLHRNLPVAEQAGQ
                     VETVKRSEAGMVTDPVTCSPDNTLAEVDAMCARFRISGLPVVDDTGELVGIITNRD"
     gene            623..1024
                     /locus_tag="VFC01_03305"
     CDS             623..1024
                     /locus_tag="VFC01_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 domain-containing protein"
                     /protein_id="HZN78706.1"
                     /translation="MRDHLPPGLPPDPFADDPCDPSAALDALEPGQPLDPQERTAVEA
                     DLADLAVYEALLAHKGIRGLVVCCDECQQDHYHDWDMLRANLLQLLVDGTVRPHEPAY
                     DPEPDAYVTWDYCRGYADASLNEATSDTDYR"
     gene            complement(1037..2212)
                     /locus_tag="VFC01_03310"
     CDS             complement(1037..2212)
                     /locus_tag="VFC01_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-D factor RsdA"
                     /protein_id="HZN78707.1"
                     /translation="MPDFGRWTNGGDPSLNEINRTDRLIDSLASQQQVYATDPAEAEL
                     AQLLAGWRDEIRTPPLMAPVTPRDATFALRRAVEARKRTRTSMAIVGSVAAAVLCLGG
                     FGAVVAGAGPGDPLYGLHTMLFGEQKPTRTDQVALASQQLAEVQQLIDEGQWQAAQDK
                     LQTLTTTVASVDDVEQKEELVSQWQQLTVKVEAQDPDATLAPGVAPPPMPEPVVVAPG
                     SDASTSTSSETSSATSSSSETSTSSSETTSSTTTTSSPTSSSPAPEPPPATGTPTTTT
                     PPTTTTTAPTTTTTTTHAVTTTTTTTQAPIAGPPVGGGAPPAGGDGDSPVGGGAQVVA
                     PPTTVPSEAPRQRGPESPAPQSPVTQNPVTEPQPVVTIPVTTTTFELPPPKKRGDGE"
     gene            complement(2205..2783)
                     /locus_tag="VFC01_03315"
     CDS             complement(2205..2783)
                     /locus_tag="VFC01_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN78708.1"
                     /translation="MTTSGERLDAVVAEAVAGNRDALREVLEIIRPIVVRYCRARVGT
                     TERSGLSADDVAQEVCLAAITALPRYKDQGRPFLAFVYGIAAHKVADAHRAAARNRSD
                     PTEVVPERFSLDAGPEQMALDAESSARMNKLLSVLPEKQREILILRVVVGMSAEETAE
                     AVGSTAGAVRVAQHRALARLKSEIIATGHDYA"
     gene            complement(2875..3648)
                     /locus_tag="VFC01_03320"
     CDS             complement(2875..3648)
                     /locus_tag="VFC01_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78709.1"
                     /translation="MSIVLANAAFGDDPQHWPLPPASTPYELWLRAVVAGGQGRYGSA
                     MSDLDAVCGLHRGGPLVSLAHSTRASFLRQLGWHDRARRWDGRAIVLAGSDPEAGADA
                     LIGLAADALGVGRFAASATALRRAAELVTGSRLPVRLAWVSAELAMARGDGATAVRQA
                     ERAADLAGTLGSARHAVKSQMVLAAALCSAGDINRSRTVADAALDAAGRLGMIPLRWA
                     LACLLADIGSAAHPAADVLRIRNECAATVRRRGGALADH"
     gene            4065..4355
                     /locus_tag="VFC01_03325"
     CDS             4065..4355
                     /locus_tag="VFC01_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HZN78710.1"
                     /translation="MPQPQQLPGPNADIWDWQMSGLCRGVDSAMFFHPDGERGRARAQ
                     REMRAKEMCRQCPVVAQCRAHALAVGEPYGIWGGLSESERELLLKRGIRRAS"
     gene            complement(4403..4918)
                     /locus_tag="VFC01_03330"
     CDS             complement(4403..4918)
                     /locus_tag="VFC01_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78711.1"
                     /translation="MIRSQLFAVAGAVTALGFLSVAGPAQAAPEACSQYAFNGDFMLQ
                     GANIGEVRVSAVPAGTRFAGETFTLADDDSTPVHGFIQDGGIQGHDINFTISWIEPSD
                     TTWTFKGTVSDDGLVHRGLMHGPGFMSLWDSTTPLACNDPGIETKPLPDDIVTPPVPA
                     TTRVPGPITAP"
     gene            5023..5484
                     /locus_tag="VFC01_03335"
     CDS             5023..5484
                     /locus_tag="VFC01_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZN78712.1"
                     /translation="MTTDDNGNEAASPEGAVTVTETGPGTYTQEIIAGHHRLNADEPR
                     PIGEDAGPTPYDLLLAGLGACTSMTVRMYADQKGWPLERVRVTLRHKRIHAEDCAECE
                     TAKGWIAHIDRDIELVGDLSDAERQRLLQIAERCPVHQTLTSEVRIATSLR"
     gene            complement(5533..6978)
                     /locus_tag="VFC01_03340"
     CDS             complement(5533..6978)
                     /locus_tag="VFC01_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HZN78713.1"
                     /translation="MELTRDRAGQPWRARQREILSRIVRETDPPTDTLSRITDPERGE
                     RDDSYNERVGEVLDLAASIGALLMASGTPATATMDQVMAITAAYGIERCEVDVINTTI
                     HVSAYLGPTQPAASTLHIVQSRSMDFSRLAAVDRLIGRIRAGQVAPSQARDELDAIIT
                     APHPYKRWIATLAWGALAFATAGTLGASLLACLVSAVSAMTIDRVNRQLNRHGLPFFF
                     QYAVGGAIATAPPLVLYWLGPKLGLQFEPTVAIAAGLVVLLAGLSLVGSVGDVITGAP
                     VTAAGRFFELVMMTGATIAGVALVLHLANRFGAPFVAIGADAPPALAELPARVAFGAA
                     SAAAYALACYAERSAAMAAAFGGAAGTIAFLLAQGAGLGAVVASFVAAVPIGLVGRLM
                     ERRNLAPPLVVSIAGIVPLLPGLALLHGIYAILNDQHAVGFASVLGAFAIGTALAAGV
                     TLGEWSSWKVRRRRLQARRRGRGTPLAGKQR"
     gene            complement(7252..7683)
                     /locus_tag="VFC01_03345"
     CDS             complement(7252..7683)
                     /locus_tag="VFC01_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZN78714.1"
                     /translation="MSADDPVTVLSEDESWRLLSSVALGRLVTVLAGQPEIFPINFIT
                     QRRTLLFRTAQGTKLYSAVMGGQVAFEADDYTADEGWSVIVRGKAHLLSANADILDAE
                     EAPLLSWPATVKPHYVRVIATEISGRRFKFGPAPDRESNFA"
     gene            complement(7746..8414)
                     /locus_tag="VFC01_03350"
     CDS             complement(7746..8414)
                     /locus_tag="VFC01_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78715.1"
                     /translation="MNLRRKRRAPPTDVVIELNNADELLAADPRGLLAGGHRIDSGMD
                     ELVERLLTQRKIAADQRVVLDIASECSDEQAAGMVSAVRRYCELRLRQANREHDLIWR
                     QGMRSLLSGSLLFVVGIALSYSFTRPVMGEFANDLLGNGVFLVVAWVGLWYPLDLLFI
                     AREQAKREIRVLGKMLTLPVIVRPHDRALPVAVLPGSPVSQGLSPSLGTRFRRFRGPS
                     KSGL"
     gene            complement(8625..>8767)
                     /locus_tag="VFC01_03355"
     CDS             complement(8625..>8767)
                     /locus_tag="VFC01_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78716.1"
                     /translation="DPVKVTRSAVLNAASVARMVLTTETAVVEKPAEEEDHGHGHHGH
                     AH"
BASE COUNT         1550 a         2881 c         2843 g         1493 t
ORIGIN      
        1 gtcgcggttg gtgatgatgc cgacgagctc gccggtgtca tcgacgacgg gcagcccgga
       61 gatccgaaac cgcgcgcaca tcgcgtcgac ctcggccagc gtgttgtcgg gcgagcaggt
      121 gaccggatcg gtgaccatgc cggcttcgga gcgcttgacc gtctccactt ggccggcctg
      181 ctcggccacc ggaaggttgc gatgcagcac gcccatgccg cccgcccggg ccatcgcgat
      241 cgccattctg gattcggtca cggtatccat cgccgagctg accaacggca ccttcagccg
      301 gatcctcttc gtcagctggc tggaggtatc ggcgttggcg ggcaccacgt cggaagcggc
      361 gggcagcagc agaacgtcgt cgaacgtgag cccgagcatc gcgaccttgg tcgggtcatc
      421 gccgccggtc ggcaccggaa cggcgatggg aatgctgctt tcagcaatcg acatgggcgg
      481 gccttccatc ggcgtcgagc tagatactca tcttatcggc attgacctcg ccacctgggc
      541 cccacacggc gttgcagatc cccaggtggg cttgccgcgc aacggctggc gcgatcccag
      601 taccgctgcg taatgtggac tcgtgcgcga tcacctccca ccgggtttgc cgcccgaccc
      661 gttcgccgac gacccatgcg acccttcggc ggcgcttgat gcgctcgagc cgggccagcc
      721 gctggacccg caggagcgga ccgccgtcga ggccgatctc gctgatctcg cggtgtacga
      781 ggcgctgttg gcgcacaagg gaattcgcgg cctggtggtg tgctgcgacg agtgccagca
      841 ggaccactac cacgattggg acatgctgcg cgccaacctg ttgcagctgc tggtcgacgg
      901 cacggtgcgg ccacacgagc cggcctacga cccagaaccc gatgcctacg tgacctggga
      961 ttactgccgc gggtacgccg acgcgtcgct caatgaagcc acgtcggaca ccgactatcg
     1021 ctaagggctg aacccgctat tcgccgtcgc cgcgcttttt cggcggcggc agttcgaatg
     1081 tcgtcgtcgt gaccggaatg gtcacgacag gctgaggctc cgtgaccgga ttctgagtca
     1141 ccggactttg cggcgcagga ctctccgggc cgcgctgacg cggggcctct gacgggaccg
     1201 tcgtcggggg cgcgacgacc tgcgcaccgc cgccgaccgg cgaatcacca tcaccacccg
     1261 cgggcggcgc gccgccaccc acaggcggac cggcaatggg cgcctgcgtt gtggtggtcg
     1321 tcgtcgtgac ggcgtgggtt gtcgttgttg tcgtcgtcgg ggcggtggtc gtcgtggtcg
     1381 gcggcgtcgt ggtcgtcggc gttccagtcg ccggtggcgg ttcaggcgcc ggcgagctgg
     1441 atgtcggcga gctcgttgtt gtggtcgacg acgtcgtctc ggacgacgag gtgcttgtct
     1501 ccgatgacga cgatgtcgcg ctgctggtct ccgacgacgt gcttgtcgat gcgtccgatc
     1561 ccggcgcgac gaccaccggc tcgggcatcg gcggcggcgc cacgcccggc gcgagggtcg
     1621 cgtccggatc ctgagcctcg accttgaccg tcagctgttg ccactgactg accagttcct
     1681 ctttctgttc aacgtcatcg accgaggcga cggtggtcgt cagcgtctgc agcttgtcct
     1741 gggcggcttg ccactggccc tcgtcgatca gctgctgcac ctcggccagc tgctgcgagg
     1801 cgagcgccac ctgatcggta cgggtcggct tctgctcgcc gaagagcatc gtgtgcagcc
     1861 cgtacagcgg atcgccggga cctgctcctg ccaccaccgc gccaaacccg ccgagacaca
     1921 acacagccgc ggcaaccgag cccacgatgg ccatcgacgt gcgggtgcgc ttgcgcgcct
     1981 cgaccgcgcg gcgcagggca aaggtggcat cgcggggtgt caccggcgcc atcagcggcg
     2041 gcgtgcggat ctcgtcgcgc caccctgcga gcagttgggc cagctcggcc tcggccggat
     2101 cggttgcgta cacctgctgc tgcgatgcca gcgaatcgat caaccggtcc gtgcggttga
     2161 tctcgttgag cgacggatcg cccccgttag tccagcgtcc gaaatcaggc atagtcgtgc
     2221 cccgtcgcga tgatttcgga tttcagccgc gcaagcgccc ggtgctgcgc gacccggacc
     2281 gcgccggcgg tggaaccgac cgcctcggcg gtttcttcgg cgctcatgcc gaccaccacg
     2341 cgcaggatca ggatttcgcg ctgcttctcg ggcagcacgg acaacagctt gttcatgcgt
     2401 gccgacgatt cggcatccag agccatctgc tcggggcctg cgtccaggga aaagcgctcc
     2461 ggcacgacct ccgtcgggtc ggaacgattc ctggcggctg cgcgatgagc gtcagcaacc
     2521 ttgtgcgcgg cgatgccata cacgaaggcc aggaacggtc gtccctgatc cttgtagcgc
     2581 ggcagcgccg tgatggcggc caagcacacc tcctgtgcga cgtcatctgc tgatagacca
     2641 cttcgctcgg tcgtcccaac ccttgcccgg cagtaccgaa ccacgatcgg gcgaatgatc
     2701 tccagcacct ctcgtagcgc gtcccgattg ccggccactg cctcagcaac gacagcatcg
     2761 agacgttctc ccgaagttgt catcgtgagc ggtatctcca gcgttacgtg agggacacgt
     2821 ccccgaagtg tggacgtcag ctaaacaata acgatccgag cggcgcgacc attgttagtg
     2881 atccgccaac gcgccaccgc ggcgacgcac agtagctgcg cattcgttac gaatgcgcag
     2941 cacatctgcg gccgggtgag cggcgctacc gatgtcggcc agcaggcagg ccaacgccca
     3001 ccgcaacggg atcattccca gccgcccggc cgcatcgagt gcagcgtcgg caaccgtgcg
     3061 ggagcggttg atatcgcctg cactgcacag ggccgcggcg agcaccatct gcgacttgac
     3121 cgcgtgccgg gccgagccca gcgtgccagc caggtcggcg gcgcgttcgg cttgcctgac
     3181 ggccgtcgcg ccgtcgccgc gggccatggc cagctcggcg gacacccatg ccagccgtac
     3241 cggcagccgc gatccggtga ccaactcagc ggcgcgccgc agcgccgtgg ccgaggccgc
     3301 gaaccggccg acgccgagcg cgtcggcggc cagcccgatg agggcgtcgg cgccggcctc
     3361 tggatccgac cccgcgagca cgatcgcgcg gccgtcccac cgccgtgctc ggtcgtgcca
     3421 gccgagctgc cgcaagaacg acgcccgggt gctgtgtgcc agcgagacca acggcccgcc
     3481 gcggtgcagt ccgcacaccg cgtccaggtc ggacatcgcg ctgccgtagc ggccctgacc
     3541 gccggccact acagcgcgca accacagttc gtatggcgtg gatgcgggcg gcagcggcca
     3601 gtgctgcggg tcgtcaccga aggcggcgtt cgccaacacg atgctcattg gatcgcgacg
     3661 ctatcaatac agtgcgcgaa acccggtacc gcggggcacg cattcaaatc cgcatgagtt
     3721 aacagttcgt ggtggccgtg ttaattcgat gtcaactggc tccggggagc gcacgagatt
     3781 ctttgccgcg cagcgagaag cgaactgtga aaacaccagt cgtgatcgca tcgccggtgt
     3841 ttcggtacca ccgatgtcgt tgccggagag tcgaaacgat gaacgtgatg taaattctcg
     3901 gcctgcggtt atgcacatga gcacatgttc gggctattga cgaaatttcg tgagcgcccc
     3961 tagtttgtgt gcacgagtag tcatcggcga cttgtgctcg gatcggttcc acgactcacg
     4021 cgcccgttcg atcgcgaaat gggtgggtag agaggggttt tcctatgcca cagccgcagc
     4081 agctacccgg acccaatgct gacatctggg attggcagat gtcaggcctg tgccgaggcg
     4141 tcgattccgc tatgttcttt catcccgacg gcgagcgtgg ccgcgcccgc gcgcagcgcg
     4201 agatgcgtgc caaggagatg tgccgtcagt gcccagtagt cgcgcaatgc cgcgctcatg
     4261 cactggccgt cggtgagccg tacggcatct ggggcggcct gtccgagtcc gagcgcgagc
     4321 tgttgctcaa gcgaggcatt cgccgcgcct catagtttct gaaggaaagg ccccacccgg
     4381 ttcgggatgg ggccttttcg cgttaagggg cggtgatggg gcccggcact ctggttgttg
     4441 ccggtacggg cggcgtgacg atgtcatccg gtaggggttt ggtttctata cccgggtcgt
     4501 tgcacgcgag cggagttgtg gagtcccaga gcgacatgaa accggggccg tgcattaagc
     4561 cgcggtgcac gaggccgtcg tcactcacgg tgcccttgaa cgtccacgtg gtgtctgacg
     4621 gttcgatcca cgagatggtg aagttgatgt cgtggccctg aatgccgcca tcctggatga
     4681 aaccgtgcac cggggtgctg tcgtcgtcgg cgagcgtgaa cgtttccccg gcaaatcggg
     4741 tacctgctgg cacggcggat accctcactt ccccgatgtt ggccccttgg agcatgaagt
     4801 cgccgttaaa ggcgtactgg ctacacgcct cgggggcggc ctgcgcgggt ccggcgacgg
     4861 agaggaaccc cagggcggtt accgccccgg caacggcgaa cagctgggat ctgatcacga
     4921 tggttcctcg cgctgcggaa gcttactctc attgaccact tcgccatcgg cgatgcagca
     4981 aacccgccga ggaccggcgt tcaaccagcc aaagtgagtt ccatgacaac agacgacaac
     5041 ggcaatgagg cggcgtcgcc cgagggcgcg gtcaccgtga cggagacggg tccgggcact
     5101 tacacgcagg agatcatcgc cgggcaccac cggctcaacg ccgacgagcc acggccgatt
     5161 ggcgaggacg cggggccaac accctacgat ctgctactgg ccgggctcgg ggcatgcacc
     5221 tccatgacgg tgcggatgta cgccgaccag aagggctggc cgctcgagcg tgttcgagtg
     5281 acgttgcgac acaagcgcat tcacgctgaa gattgcgccg agtgcgagac ggccaaggga
     5341 tggatcgctc acattgatcg cgacatcgag ctcgtcggtg atctcagcga cgctgaacgt
     5401 caacggttat tgcaaatcgc agaacgttgt cccgttcatc agaccctgac ctcggaggtc
     5461 cgcatcgcca cgtccttgcg ctgacaatga aacgcccgcc gacagaccag ggtgtcccat
     5521 ctcaactaac tattagcgtt gctttccggc gagtggtgtc ccgcgcccgc ggcggcgcgc
     5581 ctgaagtcgg cggcgccgaa ctttccacga gctccattcg cccaatgtca cgccggccgc
     5641 gagcgcggtg ccgatcgcaa atgcacccag caccgaagcg aacccgacgg cgtgctggtc
     5701 gttgagaatc gcgtagatgc cgtgcagcag cgccaagcca ggcagcaggg gcacgatgcc
     5761 cgcgatcgac accaccagcg gcggcgcgag gttacggcgt tccatcagac ggccgaccaa
     5821 gccgatcggc accgcggcga cgaacgacgc cacgacggca cctagacccg cgccctgagc
     5881 cagaaggaac gcgatggtgc ctgcggcgcc gccgaatgcg gcagccatcg ctgcggacct
     5941 ttccgcatag caggccaggg cgtaggccgc tgcgctcgcc gcgccgaatg cgacacgtgc
     6001 aggcaactct gccagtgcgg gcggcgcatc cgcgccgata gcgacgaagg gcgcaccgaa
     6061 gcggttggcg aggtgcagaa ccaacgccac acccgcgatc gtcgctcccg tcatcatgac
     6121 caactcgaag aagcggcccg cagcggtgac cggggcgccg gtgatgacgt cgccgaccga
     6181 acccaccagc gaaaggccgg caaggagcac gaccaggccc gcggcgatcg ccaccgtcgg
     6241 ttcgaactgc agacccagct tggggccgag ccagtagagc acgagcggag gcgcggtcgc
     6301 gatcgcgccg ccaaccgcgt attgaaagaa gaagggcagt ccatgccggt tgagttgtcg
     6361 attgacgcgg tcgatcgtca tggcgctcac agcgctgacc aggcaagcca gcaagctcgc
     6421 gccgagggtc ccggccgttg cgaacgccag cgcgccccag gcgagggtgg cgatccagcg
     6481 cttgtagggg tgcggcgcgg tgatgatggc gtcgagctca tcgcgggctt gcgacggagc
     6541 tacctggccc gcgcggatac ggccgatcaa gcggtcaacg gctgccagtc ggctgaagtc
     6601 catcgaccgg gattgcacga tgtgcagcgt gctggccgca ggctgcgtcg gcccaagata
     6661 ggcggagacg tggatggtcg tgttgatgac atcgacctcg caacgctcga tgccgtacgc
     6721 ggcggtgatg gccatcacct gatccatcgt cgcggtcgct ggcgttcctg acgccatcag
     6781 gagggcaccg atactcgcgg ctagatcgag cacctcgccg acgcgctcgt tgtaagagtc
     6841 gtcgcgctca ccgcgctccg gatccgttat ccgcgacagg gtatccgtcg gtgggtcggt
     6901 ttcccgaacg attcggctca agatctcgcg ttgacgtgcc cgccacggct ggcccgcacg
     6961 atcacgggtc aactccacag catttcctcc tattgcactt atgcatcgac cgactcggat
     7021 agtcccaacg cgcaggcgcg gacgacggtt ccgtttatcg cttaacgtat ttggtgtttt
     7081 cgctcacatc gccacggcgc ggtgtcccag gcagtcgcac gtaacggttg cagtttcgag
     7141 cggaatggaa cagcaactcc acattctact ttgatcgtca aatccgctgg acacgaatct
     7201 ttttgacacc aaacaaaaca gccccggtga cgaaccggaa agcaccagta atcaagcaaa
     7261 gttggattcg cgatctggcg ctggaccgaa cttgaatcgc cggccggaga tctccgtggc
     7321 gatcacccgc acatagtgag gcttcacggt cgcgggccac gacagcaacg gcgcttcctc
     7381 ggcgtccagg atatcggcgt tggctgacaa cagatgcgcc ttgcccctga cgatcacgct
     7441 ccatccctcg tctgccgtgt agtcgtctgc ctcgaaagcc acttggccgc ccatcacagc
     7501 gctgtacagc ttggtgccct gggcagttcg gaagagcagc gttcgccgtt gggtgatgaa
     7561 gttaatcggg aagatctcgg gctgcccggc caagaccgtg actaatcggc ctaacgccac
     7621 gcttgataac aagcgccagc tctcgtcttc actgagcacg gtcactggat cgtcggcgga
     7681 catggttcag cctaactcga tgtccagcac cgcagacctg acccgcaaaa cggaaccaca
     7741 tcgtcctaaa ggcccgattt ggatgggcca cgaaaacgac ggaatcgggt accgaggctt
     7801 ggcgataaac cctggcttac cggtgagccg ggtaacactg caacgggcag tgcgcggtcg
     7861 tgggggcgaa caatcaccgg gagggtgagc atcttgccga gcacgcggat ctctcgcttt
     7921 gcctgttccc gggcgatgaa caacaagtcg agcgggtacc agaggccaac ccacgcgacg
     7981 actaggaaca cgccgtttcc gagcaggtcg ttcgcgaatt cacccatcac cggtcgcgtg
     8041 aacgagtacg acaacgcgat gcccacaacg aacagcagcg aaccactgag cagcgaccgc
     8101 atgccctggc gccagatcag gtcgtgttcg cggttggctt ggcgcagtcg cagctcgcaa
     8161 tagcggcgca cggccgacac catcccggca gcctgttcgt cggagcactc gctggcgatg
     8221 tccaaaacga cgcgctggtc agcggcgatc tttctctggg tcagtaaccg ctccaccagt
     8281 tcgtccatgc cgctatcgat gcggtgccca ccggcgagca ggccgcgagg atcggccgcg
     8341 agcagctcgt cggcattgtt tagctcgatc accacatcgg tcgggggcgc tcgcctcttg
     8401 cgtcgcaggt tcactacgta acctccccgc ccggcgcgca attggccagt ccgcagtgga
     8461 tccaccatgg aaccagtaaa agtcgcaccg ttcaccaacg gcactcgcga atactacgta
     8521 ccaaggtgtc cgcaatcagg cggatcggcg tccggggcct tgccctaatg ggccgaaaaa
     8581 caaagcaccc ccggcgataa accggggtcc tccgtctcaa ttacttaatg cgcgtggccg
     8641 tgatggccgt ggccgtgatc ttcttcctcg gccggcttct cgacgaccgc cgtctcggtg
     8701 gtgagcacca tccgggccac cgacgccgcg ttcagcacgg ctgagcgggt gaccttgacc
     8761 gggtcga
//
LOCUS       DASRJA010000074        18525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_504555,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000074 DASRJA010000000
VERSION     DASRJA010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18525
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504555"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..858
                     /locus_tag="VFC01_03360"
     CDS             10..858
                     /locus_tag="VFC01_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HZN78717.1"
                     /translation="MTEVRATDPAAIDRAWQNRTTRPTVRGNGRLMIVAADHPARGAL
                     AVGSRPTAMNSRTDLLDRLRAALADPGVDGVLATADILDDLVLLGALEDKVVFSSMNR
                     GGLAGAQFELDDRMTAATAAATAAAKMNGGKMLCRIDLGDPGTVATLEACARAIDELA
                     SHGLIAMVEPFMSTRVEGKVRNDLSPDAVIKSIHIGQGLGSTSAYTWMKLPVVDEMDR
                     VMEATTLPTLLLGGDPADPDEAFASWEKALGLPSVRGLIVGRTLLYPADDDVSSAVAT
                     AVSMVR"
     gene            861..1721
                     /gene="iolB"
                     /locus_tag="VFC01_03365"
     CDS             861..1721
                     /gene="iolB"
                     /locus_tag="VFC01_03365"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HZN78718.1"
                     /translation="MQGKYYIPARSATPPYTVDVTPESAGWTESSLKVLELESPLTLD
                     TGDTEVMVLPLAGQGSVEVDGETFELSPRASVFDGPADMVYVGTGQTYTLAGEGRFAI
                     CGARARRNLPNRRVAAHDVSVELRGAGNCSRQVHNFGTAGVFEADSLIACEVITPGGN
                     WSSYPAHKHDEESPVESQLEEIYYFEIASGPGNSAGFGYHRVYGTPDRPIDVLEEVRS
                     GDVVLVPHGYHGPSIAAPGFDMYYLNVMAGPGQERAWKIVFDPHQDWLRDTWEHQDVD
                     PRLPLHTRGA"
     gene            1723..3657
                     /gene="iolD"
                     /locus_tag="VFC01_03370"
     CDS             1723..3657
                     /gene="iolD"
                     /locus_tag="VFC01_03370"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009474060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HZN78719.1"
                     /translation="MVSKASAFKSPHNEPTVRLTVAQATIRFLLNQYVERDGERAKFF
                     AGCFGIFGHGNVAGIGQALLQAEVDEDEPTLRYVLGRNEQAMVHTAAAYARQKDRLQA
                     WAVTASIGPGSTNMLTGAALATINRLPVLLLPSDTFATRVSAPVLQDLEQPHDGELTV
                     NDAFKPLSRFFDRVWRPEQLPSALLGAMRVLTDPVETGAATISLPQDVQAEAYDWPES
                     LFAERTWHVARPVPERSVITRAAEIIRSAQRPLIVAGGGVIYSGANDALAAFVEKTGI
                     PVSETQAGKGSLRYDHPQSVGAVGSTGTTAANALATEADVVIGIGTRYSDFTSASRTA
                     FNNPDVRFVNVNVASFDAVKQGGLSVVADAREALEALSAALGDYAVSAEYRSQTTELA
                     KEWDVTVSAAYRVEADGSALNQSQVIGLANTLSDPRDVVVCAAGSMPGDLHKLWRTRD
                     RKGYHVEYGYSCMGYEVAGGIGIRMAAPDRDVFIMVGDGSYLMMATELVTAVQEGVKV
                     IVVLVQNHGFASIGSLSESLGSQRFGTSYRYRSADGRLDGDKLPVDLAANAASLGAEV
                     IRVTTAAEFTDAVKVAKANEHTTVIHVETDPLVYAPDSQSWWDVPVSQVSALESTQAA
                     YETYAEWKKIQRPLVRPADS"
     gene            3678..4580
                     /locus_tag="VFC01_03375"
     CDS             3678..4580
                     /locus_tag="VFC01_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZN78720.1"
                     /translation="MSTILVGSAPDSWGVWFPDDPDQTPYTRFLDEVANSGYQWIELG
                     PYGYLPTDPQKLSDELASRNLKLSAGTVFEHLHQDNSWDAVWKQIEDVAKLTAAVGGK
                     HVVVIPEMWRDPATGEVLEDRNLTAEQWKKKTGGMNDLGKAMFEKYGVRAQYHPHADS
                     HVDTEENVYRFLDGTDSEFVNLCLDTGHISYCGGDNLAIINRAPDRIGYLHLKQVDPE
                     VRAKVEAEDLPFGEAVKLGAMTEPPRGIPEMPPLLDAIEKLGIDVFAIVEQDMYPCDP
                     DQPLPIAQRTRRYLGSCGVPSVKF"
     gene            4594..5604
                     /locus_tag="VFC01_03380"
     CDS             4594..5604
                     /locus_tag="VFC01_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN78721.1"
                     /translation="MSDLRIAVLGVGMMGADHVARITSRISGARVAVINDYLPEKAQE
                     VAASVPGARVAADPFDAINADDVDAVLLATPGPTHEKQLLACLEQGKPVLCEKPLTTD
                     STSSLEVVRREAALGTPLIQVGFMRRFDPEYHQLKLLIDGGELGQPLVMHCAHRNPAV
                     PPGFDSAMVVKDSLVHEVDVTRFLLGEEIASVQIIKPVPNRGAPRGLQDPQIAIFRTV
                     SGRHVDVELFVTTGVAYEVRTEVVGELGSATIGLDVGLVRKSSPGTWGGRITPGFRER
                     FGTAYDIELQRWVDAVSSGVNVDGPTVWDGYAAVAVCEAGVRSLETGERVDIELAERV
                     GA"
     gene            5604..6479
                     /locus_tag="VFC01_03385"
     CDS             5604..6479
                     /locus_tag="VFC01_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZN78722.1"
                     /translation="MKIALDPTPFHHDHELLEFPRVVAELGYEYLQLTPHRDFIPFFN
                     HPRADDELVAKFRKACADAGVGIASVLPVLRWSGPDEDAREAAVRNWKRVIQITVDLG
                     VNVINTEFSGRPERAEESERAFFRSMEELVPIFEREGIDVRIDPHPDDFVEDGLEAVR
                     IIRGVNSPNIGMVYVACHSYHMGGNMTEIMRAAGEKLRLVHVADTMDHHRSHGLRYIT
                     NPPGSPARVHQHLKIGDGDLNWDEFFGGLADIGFYDRPDTVMVSSVFAEDENAQDVSR
                     YQLKTMTEYISKYRK"
     gene            6482..7975
                     /locus_tag="VFC01_03390"
     CDS             6482..7975
                     /locus_tag="VFC01_03390"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZN78723.1"
                     /translation="MSKTISHWINNKAYSGTTGDTLPVTNPATGEVTGQLALATVEDA
                     RAVIDAAAAAFPAWRDTSLAKRTQILFAFRELLNVRKHELAEIITSEHGKVVSDALGE
                     VSRGQEVVEFACGIPHLLKGGFTENASTKVDVYSIRQPLGPVGIISPFNFPAMVPMWF
                     FPIAIAAGNTVVLKPSEKDPSASLWMAELWAEAGLPPGVFNVLQGDKTAVDELLTNPK
                     IKSVSFVGSTPIASYVYQTGTAAGKRVQALGGAKNHAVILPDADLDLAADAMVNAGFG
                     SAGERCMAISACVAVGPIADDLVAKIAERARTIKTGDGTGDADMGPLVTKAHRDKVAS
                     YIDAGEADGAKIVVDGRGVTAEGGKDGFWLGPTLIDNVTPEMSVYTDEIFGPVLSVVR
                     VESYDEALQLVNENPYGNGTAIFTNDGGAARRFQNEVEVGMVGINVPIPVPMAYYSFG
                     GWKASLFGDTHAHGTEGVQFFTRGKAITSRWLDPSHGGIELGFPQNK"
     gene            8057..8905
                     /locus_tag="VFC01_03395"
     CDS             8057..8905
                     /locus_tag="VFC01_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZN78724.1"
                     /translation="MTRPQLVATAWTSAGDTSPLRVPATSPVPIAERVAAVAEAGFTG
                     LGLIADDLVAVRDSIGFAALRNVITDHGLTHVEIELIERWWVPRAAAGNSYQVRELLF
                     EAADVLAPAFIKIGSELGPPTPHPEQLIPPLCELATQAAEHGTRIAIETMPFSIISTV
                     PMGADIISAAAHPGIGLLVDAWHVFRAGTTLEELRAALTPGVLFGVELDDAAQAVVGT
                     LFEDTVDHRLLCGEGSFDLRGLVALLRDKGFDGPWGVEILSSSFRRLPVSQAVKLAAE
                     SALTVL"
     gene            complement(8991..11168)
                     /locus_tag="VFC01_03400"
     CDS             complement(8991..11168)
                     /locus_tag="VFC01_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HZN78725.1"
                     /translation="MSGNAVRLQAINKVEAYVPPAISFDPGEAPGEIFGSNVFTKAEM
                     QARLPKSVFKSVVATIEKGAKLDPAVADAVAAAMKDWALEKGATHYAHVFYPMTGLTA
                     EKHDSFLEPVSDGQTLAEFAGKTLIQGEPDASSFPSGGLRSTFEARGYTGWDVTSPAY
                     ILENPNGNTLCIPTVFVSMTGEALDYKTPLLRSQQAMGVHAERILKLFGHEQLEKVVS
                     FCGPEQEYFLVDRHFFLARPDLINAGRTLFGAKPPKGQEFDDHYFGAVPERVLGFMMD
                     TERELFKLGIPAKTRHNEVAPGQFEVAPMFERANLASDHQQLLMTTFKTIAKKHGMEC
                     LFHEKPFAGVNGSGKHVNFSVGNAELGSLLVPGDTPHENAQFLVFCAAVIRAVHKYAP
                     LLRISVASATNDHRLGANEAPPAIISIFLGDQLADVFEQIAKGAATSSKGKGTMIIGV
                     DTLPPLPTDPGDRNRTSPFAFTGNRFEFRAPGSGQTVAVPMIILNTIMADSFDYIATI
                     LEKAAEDGEDFDVSVQKLLTEVITEHGAVVFNGDGYSENWQIEAAERGLPNFKTTLDA
                     LPSLITPESVELFEKYSVFNDRELHSRYEVRLEQYALTIGVEAKLTLEIGSTVVLPAA
                     VRYQTELAQNVAALKKAGGQPSTVLLEAVSEPIAELTSALADLRKVLGDHSAESAYDE
                     ALHARDALLPAMEAVRAAADTLEGVVADDLWPLPTYQEMLYIL"
     gene            11372..12469
                     /locus_tag="VFC01_03405"
     CDS             11372..12469
                     /locus_tag="VFC01_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012833794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN78726.1"
                     /translation="MRLVAGMVGGGPGAMIGAAHRHAMWLDNQYVLAAGIFGRNREAS
                     AQFADELGVERVYRDHREMVEKEAGRLDVVAVVTPNDTHFEIAKAFLEAGISVVCEKP
                     LTNDSATAAELVAIAESNNVILAVPHIYSAYPMVRHAARMVRNGDLGRIRFVAAEHAS
                     GWASADVEQWRMDPDRGGVATAVADVGTHAFHLLRYITGLEATRVSAELGTLVPNRPV
                     FDNATMRLTLSNGAPATVWATMAATGQEHGLRIRVFGDDASLEWHHEDPQHLTVRDPD
                     GGATILAHGISGLSDDAARLTRVGLGHPEGFLEAFANFYLDLAELLRGRPARDLSIPT
                     GVDGLIGVQLVEATVASHANDAAWVDTSFGT"
     gene            complement(12593..12637)
                     /locus_tag="VFC01_03410"
                     /pseudo
     CDS             complement(12593..12637)
                     /locus_tag="VFC01_03410"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12892..15210
                     /locus_tag="VFC01_03415"
     CDS             12892..15210
                     /locus_tag="VFC01_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HZN78727.1"
                     /translation="MSTRRHPDQDVRAGYDEHAVVVTEAKHEAAGVKAVLVSLRRGLE
                     SMGAVRTAAALARLNQRHGFDCPGCAWPEEHGGRKLAEFCENGAKAVAEEATKRVVTP
                     EFFARHSIADLEGRPEYWLSQQGRLTHPVVLRPTDDHYRPISWDDAYRLIADELRALA
                     SPNEAVFYTSGRTSNEAAFLYQLMVRCFGTNNLPDCSNMCHESSGAALIEAIGIGKGS
                     VTVEDVTQADLIVIAGQNPGTNHPRMLSVLEKAKANGAKIIAVNPLPEAGLIRFKDPQ
                     KVHGVIGHGVPIADEFVQIRIGGDMALFAGLGRLLLEADDAAPGTVVDREFIAKHCAN
                     FAEYEARTRTVDLDTVLEATGIDRAQLDLMAKMMAASERTIVCWAMGLTQHRHAVPTI
                     SEITNLLLMRGMIGKPGAGLCPVRGHSNVQGDRTMGIWEKMPEQFLAALDDRFGIVSP
                     REHGVDTVDAIRAMRDGRAKVFMGMGGNFAMATPDTAVTEAALRGCSLTVQVSTKLNR
                     SHVVHGATALILPSLGRTDRDIQNGTKQQVSVEDSMSMVHLSRGSLHPPSDQVRSEVA
                     IVCQLAHALFGSDHPVPWEQFTGDYDAIRDAIAAVVPGCEDYNRKVRAPDGFQLPHGP
                     RDAREFPTSTGKANFSADPLEWVPVPEGRLVLQTIRSHDQYNTTIYGLDDRYRGVKGG
                     RRVIFVHPADIERFGLTDGDRVDLVSEFTDGDGHVQERRAKDFMVVAYSTPQGNAAAY
                     YPETNPLVPLDHTAAKSNTPVSKAIVIRLERT"
     gene            15210..16064
                     /gene="fdhD"
                     /locus_tag="VFC01_03420"
     CDS             15210..16064
                     /gene="fdhD"
                     /locus_tag="VFC01_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZN78728.1"
                     /translation="MGRVTARRRVQHVTADDAVARPDTLVVEEPLEIRVNGSPITVTM
                     RTPGSDIELAQGFLLTEGVIAGRDDVVTVRYCKGATDEGVNTYNVLDVTLAPGVQMPD
                     VDVTRNFYTTSSCGVCGKASLDAVRLISRHCPGDDPSTVAAETLSAMPEKLRDAQKVF
                     ASTGGLHGAALFGVDGTTLAVREDIGRHNAVDKVIGWALEADRIPLTSTVLLVSGRAS
                     FELTQKAVMAGIPVLAAVSAPSSLAVDLASQSGLTLVAFLRGDSMNVYTRPDRVTPRG
                     MSVTKSGR"
     gene            complement(16242..18443)
                     /locus_tag="VFC01_03425"
                     /pseudo
     CDS             complement(16242..18443)
                     /locus_tag="VFC01_03425"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
BASE COUNT         3354 a         6081 c         6044 g         3046 t
ORIGIN      
        1 tacaccgaga tcacggaagt gcgcgccacc gatcccgccg cgatcgaccg cgcgtggcag
       61 aaccgcacca cccggccgac cgtgcgcggc aacggcaggt tgatgatcgt cgccgccgac
      121 caccccgccc gcggcgcgct cgcggtgggc tcgcggccca cggcgatgaa cagccgcacc
      181 gaccttctcg accggctccg cgccgcgctc gccgatcccg gtgtggacgg cgtgctggcc
      241 accgcagaca tcctcgacga tctggtgctg ctcggtgcgc tggaagacaa ggtggtgttc
      301 tcgtcgatga accgcggcgg gcttgccggc gcgcagttcg aactcgacga caggatgacc
      361 gccgccaccg ccgcagccac cgccgcggcg aagatgaacg gcggaaagat gttgtgccgc
      421 atcgatctcg gcgatcccgg tactgtcgcc actctcgagg cgtgcgcccg ggcgatcgac
      481 gagcttgcgt cgcatgggct gatcgccatg gtcgagccgt tcatgtcgac acgcgttgag
      541 ggcaaggttc gcaacgacct gtcccccgac gccgtgatca agtcgattca catcgggcag
      601 ggcctcggct cgacgtcggc gtacacctgg atgaaattgc cggtggtaga cgagatggac
      661 cgggtgatgg aagcgacgac gctgccgacc ctactgctcg gcggcgatcc cgccgaccct
      721 gatgaggcat tcgccagctg ggagaaggcc cttgggctgc cgtcggtgcg cggcctgatt
      781 gtcggccgca ccctgctcta tccggccgac gacgatgtca gctccgcggt cgcgaccgcc
      841 gtctcgatgg tgaggtgaac gtgcaaggca agtactacat ccccgcccgc agcgccacgc
      901 ccccgtacac cgtcgacgtc accccggagt cggcgggttg gaccgaatcc agcctgaaag
      961 tgctggaact ggagtctccg ctcacgctgg acaccggcga caccgaggtg atggttctgc
     1021 cgctggccgg ccaggggtct gtcgaagttg acggtgagac gttcgaacta tccccgcggg
     1081 catcggtttt cgacggtccg gccgacatgg tctacgtcgg caccggccag acctacaccc
     1141 tggccggcga gggccggttc gcgatctgcg gcgcccgggc gcggcggaac ctgccgaacc
     1201 ggcgggtcgc ggcccacgac gtgtcggtcg aactgcgcgg tgcgggcaac tgcagccgcc
     1261 aggtgcacaa cttcggcacg gcaggggtat tcgaggccga ttcgttgatc gcgtgcgagg
     1321 tgatcacgcc gggcggcaat tggtcgagct accccgccca caaacacgac gaggagtccc
     1381 cggtcgaatc gcagctcgaa gagatttact acttcgagat tgcctccggc ccaggcaatt
     1441 cggccggatt cggctaccac cgcgtgtacg gcactccgga ccggccgatc gatgtgctcg
     1501 aagaggtccg ctccggcgac gtcgtcctcg tgccgcacgg ctatcacggt ccgtcgatcg
     1561 ccgcgccagg ttttgacatg tactacctca acgtgatggc cggccctggc caggagcggg
     1621 cctggaagat cgtcttcgac ccgcaccagg actggttgcg cgacacctgg gagcatcagg
     1681 acgtcgaccc ccggctccca ctgcacaccc gaggagcgtg acgtggtttc caaagcatcc
     1741 gcgttcaagt cgccgcacaa cgagccgacc gtgcgactca ccgtcgcgca ggccaccatt
     1801 cgattcctcc tcaaccagta cgtcgagcgt gacggtgaac gcgccaagtt cttcgccggc
     1861 tgcttcggca tcttcggcca cgggaacgtg gccggcatcg ggcaggcgtt gctgcaggcc
     1921 gaggtcgacg aggacgagcc cacgctgcga tatgtgttgg gccgcaacga acaagcgatg
     1981 gtccacaccg cggccgccta tgcgcggcag aaggatcggc tgcaggcgtg ggcggtgacg
     2041 gccagcatcg gcccagggtc gacgaacatg ctgaccggtg ccgcgctggc caccatcaac
     2101 cggttaccgg tgctgctgct gccgtcggac accttcgcca cccgagtcag tgcgcccgtt
     2161 ctgcaggacc tcgaacaacc acacgatggc gagctgaccg tcaacgacgc gttcaagccg
     2221 ctgtcgcgct tcttcgaccg ggtgtggcgt ccagagcagc tgccgtcggc actgttgggc
     2281 gcgatgcgcg tgctcacgga tccggtcgag accggtgcgg ccacgatttc actgccgcag
     2341 gacgtgcagg ccgaggcgta cgactggccg gaatcgctgt ttgccgaacg gacttggcac
     2401 gtcgcacgcc ccgtgcctga gcgctcggtg atcaccaggg ccgcggagat catccggtcg
     2461 gcccaacgac cgttgatcgt ggcgggcggc ggtgtcatct actccggcgc caacgacgca
     2521 ctggccgcgt ttgtcgagaa gacaggcatt ccggtatcgg agacgcaggc cggcaaggga
     2581 tcgctgcgct acgaccatcc ccagtcggtc ggcgcggtcg gttcgacggg cacgaccgcc
     2641 gccaacgcgc tggccacaga ggccgacgtg gtgatcggga tcggcacgcg ctacagcgat
     2701 ttcaccagcg cctcgcggac ggcgttcaac aatcccgacg tgcggttcgt caatgtcaac
     2761 gtcgcgtcgt tcgacgccgt caaacagggc gggttgtcgg tggtggccga tgcccgcgag
     2821 gcgctggaag ccctgtcggc cgcgctgggc gactacgcgg tgagcgccga gtaccgctcg
     2881 cagacaactg aactcgccaa ggagtgggat gtgacggtgt cggcggccta ccgcgtcgag
     2941 gccgacggca gcgccctgaa ccaaagccag gtgattgggc tcgcgaacac gctgtccgat
     3001 ccacgggacg tggtggtgtg tgcggccggt tcgatgcccg gcgacctgca caagctctgg
     3061 cgcacccgcg atcgcaaggg ttaccacgtc gaatacggct actcctgcat gggatatgag
     3121 gtggccggcg gcatcggcat ccgaatggcc gcccctgacc gcgacgtgtt catcatggtc
     3181 ggcgacggct cgtatctgat gatggcgacg gaactggtca ccgcggtcca agagggcgtg
     3241 aaggtcatcg tcgtgctggt gcagaaccac ggcttcgcat cgatcggctc gctctcggaa
     3301 tcgttgggct cacagcgttt cggcacctca tatcgctacc gcagcgcgga cggacggctc
     3361 gacggcgaca agctgcccgt cgaccttgcg gccaacgccg ccagcctggg tgccgaagtg
     3421 atccgcgtga cgaccgccgc cgagttcacc gatgcggtca aggtggccaa ggccaacgag
     3481 cacaccaccg tcatccatgt cgagaccgac ccgttggtgt atgcgccgga cagccaatcg
     3541 tggtgggacg ttccggtgag ccaggtttcg gccctggagt ccacccaagc agcctacgag
     3601 acgtatgccg agtggaagaa gatccagcgc ccgctggtgc gacccgccga cagctaaaca
     3661 gaaaggacta ccgatagatg agcacgatcc ttgtcggctc ggcacccgac tcgtggggcg
     3721 tatggttccc cgacgacccc gaccagacgc cgtacacccg attcctcgac gaggtggcca
     3781 actccggcta ccagtggatc gagttgggcc cgtacggcta tctgcccacc gacccgcaga
     3841 agttgtccga cgaactcgcg tcacgcaact tgaagttgtc cgccgggacg gtgttcgagc
     3901 atctgcacca agacaattcg tgggatgcgg tgtggaagca gatcgaggat gtggccaagc
     3961 tgaccgcggc cgtaggcggc aagcatgtcg tcgtcattcc cgagatgtgg cgcgaccccg
     4021 ccaccggcga ggtgctagag gatcgcaacc tcaccgccga acagtggaag aagaagaccg
     4081 gcggcatgaa cgacctcggc aaggcgatgt tcgaaaagta cggcgtgcga gcccaatatc
     4141 acccgcacgc cgacagccac gtcgataccg aagagaatgt ttaccgtttc ctcgacggca
     4201 cggactccga attcgtgaac ctctgtctgg acaccggcca catcagctac tgcggcggtg
     4261 acaacctggc gatcatcaac cgtgcgcccg accggatcgg gtacctgcac ctcaagcagg
     4321 tcgatcccga ggtgcgcgcc aaggttgagg cggaagatct gccgttcggc gaggcggtca
     4381 agctcggtgc gatgaccgaa ccaccgcgtg gcatcccgga gatgccgccg ctgctcgacg
     4441 cgatcgagaa gctcggcatc gacgtgttcg cgatcgtcga gcaggacatg tatccgtgtg
     4501 atcccgatca accgctgccg atcgctcagc gcacccgccg ctacctcggg tcgtgcggtg
     4561 ttccgtccgt gaagttctag gagttcaaaa gtcatgtcag atctgcgtat tgccgtgctt
     4621 ggcgtcggga tgatgggcgc cgaccatgtc gcccgcatca ccagtcgaat cagcggcgca
     4681 cgcgtcgccg tcatcaacga ctacttaccg gagaaggccc aagaggtcgc cgcgtcggtg
     4741 cccggtgccc gggtggctgc ggatccgttc gacgcgatca acgccgacga cgtcgatgcg
     4801 gtgctgctcg cgacgccggg tccgacgcat gaaaagcagc tgctggcctg tctcgaacag
     4861 ggcaagccgg tgctgtgcga gaaaccgttg accacggact cgacatcctc gctggaagtg
     4921 gtgcgccggg aggcggcgct ggggacaccg ctcattcagg tcggcttcat gcgccgcttc
     4981 gacccggagt accaccagct caagttgctg atcgatggcg gcgaactcgg ccagccgttg
     5041 gtgatgcact gcgcacaccg caaccccgcg gtgccaccgg gtttcgactc cgcgatggtg
     5101 gtcaaggact cgctggtaca cgaggtcgac gtgacccgct tcctgctcgg cgaggagatc
     5161 gcctcggtgc agatcatcaa gccggtgccg aatcggggtg caccgcgcgg gctgcaggac
     5221 ccgcagatcg cgatcttccg gacggtcagc ggcaggcacg tcgacgtcga gctcttcgtc
     5281 acgacaggcg tcgcatacga ggtgcgtacc gaggtcgtcg gcgaactggg cagcgccaca
     5341 atcggtctgg acgtcggatt ggtccgcaag tccagccctg gcacgtgggg aggacggatc
     5401 acgcctggct tccgtgaacg cttcgggacg gcgtacgaca tcgaactgca gcggtgggtc
     5461 gacgccgtca gctccggcgt caacgtcgac gggccgacgg tgtgggacgg ctacgccgcc
     5521 gtagcggtgt gcgaagcagg ggtccggtcg ctggagaccg gtgagcgcgt cgacatcgaa
     5581 ctcgcggaaa gggtgggtgc ctgatgaaga tcgcgttgga ccccaccccg ttccaccacg
     5641 accacgagct gctcgagttc ccgcgcgtgg tcgcggaatt gggatacgag tacctgcagc
     5701 tcacgccgca ccgggacttc atcccgttct tcaaccaccc ccgcgccgac gacgaactgg
     5761 tcgcgaagtt ccgcaaagca tgcgcggacg cgggtgtggg aatcgcgtcg gtgctgccgg
     5821 tgctgcgctg gtccggcccc gacgaggacg cccgcgaagc cgcggtgcgt aactggaagc
     5881 gcgtcatcca gatcaccgtc gacctcgggg tcaacgtgat caacaccgag ttctccggcc
     5941 ggcccgaacg ggccgaggag tcggagcgag cgttcttccg gtcgatggaa gagctggtgc
     6001 cgatcttcga acgcgagggc atcgacgtcc gaatcgaccc gcacccagac gatttcgtcg
     6061 aagacgggct ggaggcggtg cgcattatcc gcggggtcaa ctcccccaac atcggcatgg
     6121 tgtacgtcgc ctgccacagc taccacatgg gcggcaacat gaccgagatc atgcgtgccg
     6181 caggcgaaaa gctgcgactg gtgcacgtag ccgacaccat ggatcaccac cgcagccacg
     6241 gcctgcgata catcaccaat ccgccgggca gccccgcccg ggtgcaccag cacctcaaga
     6301 tcggcgacgg cgacctgaat tgggacgaat tcttcggcgg gctcgccgac atcggcttct
     6361 acgaccggcc agacaccgtc atggtgtcgt cggtgttcgc cgaagatgag aacgcccagg
     6421 acgtctcgcg ctaccaactg aagaccatga ccgaatacat ctcgaagtac agaaagtgat
     6481 tatgagcaag accatttcgc attggatcaa caacaaggcc tattccggca ccacgggcga
     6541 cacgctgccc gtgaccaatc cggcgacagg tgaggtcacc gggcagctgg cgctggccac
     6601 cgtagaggat gcgcgcgcgg tgatcgacgc tgctgcggcg gcgttcccgg cgtggcgtga
     6661 cacgtcgctg gccaagcgca cccagatcct gttcgcgttc cgcgagctgc tgaacgtccg
     6721 caaacacgag ctggccgaga tcatcacgtc cgagcacggc aaggtggtct ccgacgcgtt
     6781 gggtgaagtg tcacgtggcc aagaggtcgt cgaattcgcc tgcggcatcc cacatctgct
     6841 caagggcggc ttcaccgaaa acgcctcgac caaggtcgac gtgtactcga tccgccagcc
     6901 gttgggcccg gtcggcatca tcagcccgtt caacttcccg gccatggtgc cgatgtggtt
     6961 cttccccatc gcgatcgccg ccggcaacac cgtcgtgctc aaaccatcgg agaaggatcc
     7021 gtcggcgtcg ctgtggatgg ccgagctgtg ggccgaagcc ggcctgccgc caggcgtgtt
     7081 caatgtgctg cagggcgata agaccgccgt cgacgaacta ctcaccaacc ccaagatcaa
     7141 gtcggtgtcc ttcgtcggct ccaccccgat cgcgtcctac gtctaccaaa ccggcaccgc
     7201 ggccggcaag cgggtgcagg ccctgggcgg cgccaagaac cacgcggtga tcctgccgga
     7261 tgccgacctg gacctggccg ccgacgcgat ggtcaacgcc ggcttcggct cggcgggcga
     7321 acgttgcatg gccatctccg cgtgtgtggc ggtcggcccg atcgccgatg atctggtcgc
     7381 caagatcgcc gaacgcgccc ggaccatcaa gaccggtgat ggcaccggtg acgccgacat
     7441 gggcccattg gtgaccaagg cccaccgcga caaggtggcg tcctacatcg acgccggcga
     7501 ggccgacggc gccaagatcg tcgtcgacgg ccgcggcgtg acggccgaag gcggtaagga
     7561 tggcttctgg ctgggcccga cgctgatcga caacgtcacc ccggagatga gcgtctacac
     7621 cgacgagatc ttcggaccgg tgctctcggt ggtgcgcgtc gagtcgtatg acgaagcgct
     7681 gcaactggtc aacgagaacc cgtacggcaa cggcaccgcg atcttcacca acgacggcgg
     7741 cgcggcccgg cgcttccaga acgaggtcga ggtcggcatg gtcggcatca acgtgcccat
     7801 ccccgtgccg atggcctact acagcttcgg cggctggaaa gcctcactgt tcggcgacac
     7861 ccacgcccac ggcaccgaag gcgtgcagtt cttcacccgc ggcaaagcca tcaccagccg
     7921 ctggctcgac cccagccacg gcggcatcga actcggcttc ccccaaaaca agtaggcgct
     7981 gtcatcgaga gggcagattg tcgattcgac tgaaatcgcg atctgaccgc agtttcgagc
     8041 gccacggagg agcgacgtga cccgacccca gctggtggcg acggcgtgga ccagtgccgg
     8101 tgacacctcg ccgctgcgcg tgcccgcgac gagccccgtt ccgatcgcgg aacgtgttgc
     8161 tgcggtcgcc gaggccggct tcaccggctt ggggctcatt gccgacgacc tagtcgccgt
     8221 ccgcgacagc attggtttcg ccgcactgcg aaacgtgatc accgaccacg gcctcactca
     8281 cgtcgagatc gaactcatcg aacggtggtg ggtccctcgc gccgcagcgg gcaactcata
     8341 tcaggttcgc gagctgctgt tcgaagcggc ggacgtcctg gcaccggcgt tcatcaagat
     8401 cggatccgaa ctggggccac ccacaccaca cccggagcag ttgatcccgc cgttgtgcga
     8461 actggccacg caggcagccg aacacggcac ccgaatcgct atcgagacca tgccgttctc
     8521 gatcatctcg accgtcccga tgggcgcgga tatcatctcg gccgcggctc atcctggaat
     8581 cggattgctg gtggatgcct ggcacgtatt ccgcgcaggc acgacgctcg aggaactgcg
     8641 ggcggcactg acacccggag tgctcttcgg cgtcgagctc gacgacgccg cccaggctgt
     8701 ggtcggaacg cttttcgaag acacggtcga ccatcgattg ctgtgcggtg aaggctcgtt
     8761 tgaccttcgc ggcctggtgg cgctcttgcg cgacaagggt ttcgacgggc cgtggggcgt
     8821 ggaaatcctg tcctcctcgt tccgacggct tccggtatcg caagccgtca agctggccgc
     8881 cgagtccgcg ctcaccgtgc tctgagcgat gctgaggatc gccgggtgtg ggggttgcga
     8941 gttaccagca cccgtacgga cgggcggacc ccacacccgg cgacggctga tcagaggatg
     9001 tacaacatct cctgataggt gggcagcggc cacagatcat cggccacaac gccttccagc
     9061 gtgtcagcgg cggcacgcac cgcctccatc gccggcagca acgcatcgcg ggcgtgcagc
     9121 gcctcgtcgt aagcactctc ggcggagtgg tcgccgagca ccttcctcag atccgcgagc
     9181 gcactggtca attcggctat cggttcggaa accgcttcca gcagaacggt actcggctgg
     9241 ccaccggcct tcttcagcgc ggccacattc tgtgccagct ccgtctgata acgcaccgcc
     9301 gcgggcagca ccaccgtcga gccgatctcg agcgtcagct tggcctcgac cccgatcgtg
     9361 agcgcgtact gctccaacct gacctcgtac cgactatgca attcccgatc gttgaaaacg
     9421 ctgtacttct caaacagttc gacagactcc ggagtgatca gggacggcag cgcgtccagc
     9481 gtggtcttga agttcggcag gccacgttcc gcggcctcga tctgccagtt ctccgagtag
     9541 ccgtcgccgt tgaacaccac cgcaccgtgc tcggtgatga cttccgtgag tagcttctgc
     9601 acggacacgt cgaagtcctc gccgtcctca gccgccttct cgagaatcgt ggcgatgtaa
     9661 tcgaacgagt cggccatgat cgtgttgaga atgatcatgg gcaccgcgac cgtctggccc
     9721 gagccgggcg cgcggaactc aaacctgttg ccggtgaatg cgaatgggct cgtgcggttg
     9781 cgatcgccgg ggtcggtcgg cagtggcggc agggtgtcga cgccgatgat catcgtgccc
     9841 ttgcccttcg acgacgtcgc cgcgcccttg gcaatctgct cgaaaacgtc ggccagctgg
     9901 tcgccgagga agatcgagat gatcgcgggc ggcgcctcat tggcgccgag ccgatggtcg
     9961 ttggtggccg atgcgaccga gatacgcagc agcggtgcgt atttgtggac cgcgcggatg
    10021 accgctgcgc agaacaccag gaactgggca ttctcatgcg gggtgtcgcc tggcaccagc
    10081 aggctgccga gctcggcgtt gcccacggag aagttgacgt gcttgcccga accgttgacg
    10141 ccggcgaacg gcttctcgtg gaacagacat tccatgccgt gcttcttggc gatggtcttg
    10201 aaggtggtca tcagcaactg ctggtgatcg gacgcgaggt ttgcgcgttc gaacatcggc
    10261 gcgacctcga actggcccgg ggccacctcg ttatgccggg tcttggccgg gatgccgagc
    10321 ttgaacagct cgcgctcggt gtccatcatg aagcccagca cccgctcggg cactgccccg
    10381 aaatagtggt catcgaactc ctgcccctta gggggcttgg cgccgaacag ggtccggccg
    10441 gcattgatga ggtccggtcg cgcgaggaag aaatgccggt cgaccaggaa gtactcctgt
    10501 tccggtccgc agaacgacac caccttctcg agctgctcgt ggccgaatag cttcaatatg
    10561 cgttccgcgt gcacacccat cgcctgctga ctgcgcagca gcggcgtctt gtaatcaagg
    10621 gcttcgccgg tcatcgacac gaacaccgta ggaatgcaaa gcgtattgcc gttcgggttc
    10681 tcgaggatgt aggccgggct ggtgacatcc cagcccgtgt agccgcgggc ctcgaaggtg
    10741 ctgcgcaggc caccggaggg gaaactcgac gcgtccggct cgccctggat cagggttttg
    10801 ccggcgaact cggccagcgt ctggccgtcg gaaacgggct caaggaagct gtcgtgcttc
    10861 tcggcagtca accccgtcat cggatagaac acgtgggcgt agtgggtggc ccccttctcc
    10921 agcgcccagt ccttcatcgc ggccgccacc gcgtcggcga ctgccgggtc gagtttcgcg
    10981 cccttctcga tcgtcgcgac cacggatttg aagaccgact tgggcagccg cgcctgcatc
    11041 tcagccttgg tgaagacgtt cgatccgaag atctcacccg gcgcctcgcc gggatcaaag
    11101 ctgattgcag gaggcacata cgcctcgacc ttgttgatcg cctgcaggcg taccgcattt
    11161 ccgctcaatt gagttcctat caccccagat gcgtcgcatc tgatttgctc gacgttgacc
    11221 ggcccacact agggaccatc gatggcaaac ttgttacgcc gccgtcaaca ccacgatgcg
    11281 gacgcgcgcg atcgcgcacg agcgtgcgcg gaatgtccaa aaattacggc gtgtctgcgt
    11341 gcagacgcgc acgctcgcgg aaaatgaaca gatgcgactg gttgcgggca tggtgggtgg
    11401 cgggcctggt gcgatgattg gcgccgctca ccgccacgcc atgtggctgg acaaccaata
    11461 cgtactagct gcaggcattt tcggccgaaa ccgtgaggcg tcggcccaat tcgcggacga
    11521 actcggtgtc gagcgtgtct atcgcgatca ccgggaaatg gtcgagaaag aagccgggcg
    11581 tctcgatgtc gtcgcggtgg ttacgccgaa cgacacccat ttcgagatcg ccaaggcgtt
    11641 cctggaggcc ggcatctcgg tggtatgcga gaagccgttg accaacgact ccgccacagc
    11701 ggccgaactt gtcgcgatcg ccgaatcgaa caacgtgatt ctggccgtgc cccacatcta
    11761 ttcggcctac cccatggtcc ggcacgccgc ccggatggtg cgtaacggcg acctcggccg
    11821 gatccggttc gtcgccgcag aacacgcatc gggctgggcg tcggcggacg tcgagcagtg
    11881 gcggatggat cccgaccggg gaggcgtcgc caccgccgtc gccgacgtcg gcacccacgc
    11941 gtttcacctg ctgcgctaca tcaccggcct cgaggccacc cgcgtctcgg ccgaactcgg
    12001 cacactggtg ccgaacagac cggtgttcga caacgcgacc atgcggctca cgctgtccaa
    12061 cggcgcgccg gcaaccgtgt gggcgacgat ggcggccact ggccaggagc acggtctgcg
    12121 gatccgggtc ttcggcgacg acgcgagcct ggagtggcac cacgaggatc cgcagcacct
    12181 gaccgtgcgc gatcccgacg gcggcgcaac gattctcgcc cacggcataa gcggcctgtc
    12241 cgacgacgcc gcccgcctca cccgcgtcgg actcggccac cccgagggct tcctggaggc
    12301 attcgccaac ttctatctgg accttgccga gctgctgcgg ggccgcccgg ccagagatct
    12361 gtccatcccc accggcgtcg acggtctcat cggcgttcag ctcgtcgaag ccaccgtcgc
    12421 ctcgcacgcc aacgacgcag cctgggtcga cacctccttc ggaacctgac gacccgtcag
    12481 tccgggcgaa ccgtgctaag gcagaacgga tgtccgacca ggtcgatgag aacccgccac
    12541 tgtccggcgc gggctgatgc tcggcctcga tggcacccag cgcgaccgcg gccgtcaccg
    12601 cagaatcgag gtcgtccacg gccaggtcga agtgcatctg ctggtgttgc ggtccgtgcg
    12661 gccatgacgg cgcggcgaag gcgtcgacgc gcaagcgtca ccatggggcc ggcgcccgcc
    12721 agacagatga ccccgcgatc agcaggtcgc ggtagaagac tgcgagggcg tcggggtccg
    12781 ggcagtcgat cgaaatggaa gcgagggtcc ctaccggcgg cgtcacgagc ctcaagggta
    12841 gagaccgggc ggtctgtgca gatcatgacg tcacagtcct aggctgggcg tatgagcacc
    12901 cgtcgccacc ccgaccaaga cgtccgcgcc ggctatgacg agcacgcggt ggtcgtgacc
    12961 gaggcgaagc atgaggccgc cggcgtcaag gcggtgttgg tgtccctgcg ccgcgggctc
    13021 gagtcgatgg gtgcggtgcg caccgccgcg gcgctggccc ggctcaacca acggcacggc
    13081 ttcgactgcc cgggctgcgc atggccggag gagcacggcg gccgcaaact cgccgagttc
    13141 tgcgagaacg gcgccaaggc cgtcgccgag gaggccacca agcgggtcgt cacgccggag
    13201 ttcttcgccc ggcactcgat cgctgacttg gagggccgcc ccgagtattg gctgtcgcaa
    13261 cagggccggc tcacccatcc cgtggtgctg cgccccaccg acgaccacta ccggccgatc
    13321 agttgggacg acgcgtaccg gttgatcgcc gacgagctgc gcgcgcttgc cagtccgaac
    13381 gaggcggtgt tttacacgtc gggccggacc agcaacgagg ccgcgtttct gtatcagctg
    13441 atggtccgct gcttcggcac caacaacctg ccggactgtt ccaacatgtg ccacgagtcc
    13501 tcgggcgccg cgttgatcga ggcgatcggg atcggcaagg gctcggtcac ggtcgaggac
    13561 gtcacccagg ccgacttgat cgtgatcgcc ggccagaatc cgggcaccaa ccatccgcgg
    13621 atgctgtcgg tgctcgaaaa ggcgaaggcc aacggcgcga agatcattgc ggtcaatccg
    13681 ctgcctgagg cggggctgat ccggttcaag gatccgcaga aggtacacgg ggtgatcggc
    13741 cacggtgtac cgatcgccga cgagttcgtg cagatccgca tcggcgggga catggcgttg
    13801 ttcgccgggt tgggccggct gctgttggag gccgacgacg cggcgcccgg cacggtggtt
    13861 gaccgcgagt tcatcgcaaa gcattgcgcc aacttcgccg aatacgaggc ccgcacccgc
    13921 accgtcgatc tcgacaccgt gctcgaagcc acgggcatcg accgggcgca gctggacttg
    13981 atggcgaaga tgatggccgc gtccgagcgc acgatcgtct gctgggcgat gggcctgacg
    14041 cagcaccggc acgccgtgcc gacgatctcg gagatcacca atctgctgct gatgcggggc
    14101 atgatcggca agcccggcgc cggattgtgc ccggtgcgtg gacattcgaa tgtgcagggc
    14161 gaccgcacga tgggcatctg ggagaagatg cccgagcaat ttcttgcggc gcttgacgac
    14221 cggttcggca tcgtcagccc ccgcgagcac ggtgtggaca ccgtcgatgc gatccgggcg
    14281 atgcgcgacg ggcgcgccaa ggttttcatg ggcatgggtg gcaactttgc gatggccacg
    14341 ccggataccg ccgtcaccga ggcggcgctt cgcggctgct cgctgacggt tcaggtgtcg
    14401 acgaagctga atcgcagtca cgtggtccac ggtgctacgg cgttgatcct gccgtcgctg
    14461 ggccgcaccg accgcgatat ccaaaacggc accaaacagc aggtgtcggt ggaggattcg
    14521 atgtcgatgg tgcatctgtc gcggggcagc ctgcacccgc cgagcgatca ggtgcgcagc
    14581 gaggtcgcga ttgtctgcca gttggcccac gctctgttcg ggtcggatca cccggtgccg
    14641 tgggaacagt tcacaggcga ctacgacgcc atccgcgacg cgatcgcggc cgtggtgccg
    14701 ggctgtgagg attacaatcg caaggtccgc gcacctgacg gcttccagct gccgcatggg
    14761 ccgcgggatg cgcgcgaatt ccccaccagc acaggaaaag ccaacttttc agcggatccg
    14821 ctggagtggg tgccggtacc cgaaggtcgg ctggtgttgc agacgatccg gagccacgac
    14881 cagtacaaca ccacgatcta cggcctcgac gaccgctacc gcggcgtcaa gggcggcagg
    14941 cgcgtcatct tcgtccaccc cgccgacatc gaacgcttcg ggctcaccga cggcgaccgc
    15001 gtggacctgg tgtccgaatt caccgatggt gacgggcacg tccaggagcg tcgcgccaag
    15061 gacttcatgg tggtcgcgta ttccaccccg caaggcaacg ccgcggccta ctatccggag
    15121 accaatcccc ttgtgccgct ggatcacacc gctgcgaagt cgaatactcc agtgtccaag
    15181 gccatagtca tcaggctgga gcgaacctag tgggtagagt gacggcccgg cgccgggtgc
    15241 aacacgtgac cgccgacgac gcggtcgcgc ggcccgacac gctcgtcgtc gaagaaccgc
    15301 tggagatccg ggtcaacggc tccccgatca cggtcaccat gcgcacgccg ggatcggata
    15361 tcgaactggc gcaaggcttt ctactcactg aaggtgtgat cgccgggcgc gacgacgtgg
    15421 tcaccgtgcg gtactgcaag ggtgccaccg acgagggtgt caacacctac aacgtgctcg
    15481 acgtgacact ggcaccaggc gtccagatgc ccgacgtgga tgtcacccgc aacttctaca
    15541 ccacctcgtc gtgcggggtt tgcggaaaag cgtcactgga cgccgtgcgc ctgatcagcc
    15601 gccattgccc cggtgacgac ccgtcgacgg tcgccgcgga gaccctgtcg gcgatgccgg
    15661 agaaactgcg tgacgcgcag aaggtgttcg ccagcaccgg cggactgcac ggcgccgcgc
    15721 tgttcggtgt cgacggcacc acgctggccg tccgcgaaga catcggccgt cacaacgccg
    15781 tcgacaaggt catcggctgg gcattggaag ccgaccgcat cccgctcacc tcgaccgtgc
    15841 tgctggtcag cggtcgcgcg tcattcgaac tcacgcagaa ggccgtgatg gcgggcatcc
    15901 cggtgctggc cgcggtgtcg gcaccgtcct ccctggcggt cgacctggcg agccagtccg
    15961 ggctgaccct ggtggcgttc ctgcggggtg actcgatgaa cgtctacacc cgccccgacc
    16021 gggtaacccc gcgcgggatg tcggtcacga aatccgggcg ctgatttcgc cggacgccta
    16081 tcaattctga ccgtcggctg gtcgattcgt cgcatcggat ggccagattg ccacgcgggt
    16141 gccgttgtgt ggtaagcgct ttcgaaccgg agagtccggc agccgatccg gaccgacgct
    16201 cccgggcgcc ataccgcagg ttgccaaggg gtcagccggc tccagccacg cgttgccggt
    16261 tcgcgaagga ggacgccgtt tctcgaacca acgtcgacga cgaacacctg gccattcaca
    16321 cgtcgaatct cgatgtgggc tcgcgacatg ccgctcgaca gtcctcggat gcgtatgaga
    16381 cgaaggccgc tccgcacgac gttcgaatcc tgcggagccc ggccgatcac gcagtctcgg
    16441 tcgatctcga atcgggaatt gtcttcgagg acgatgtacc cgaacggtcg gcggatgcgg
    16501 accgtggcgg cggtgcaaga gggatacgcc cagccgggga ccgtgggttt cggcaagcga
    16561 acagtctcgg gctcaggtgc ccgctgcacc ggcgctggga tcgcaggggc ttcgctgaag
    16621 ccgatgtggt cgtgtagcca cttgagcggc cgtggtgccc accagttgaa atcgccggcg
    16681 acgcgcagga acgcgggcac gatgacaccg cggatgaccg tggcgtcgag tatgactgcc
    16741 agagccgtgc ctatgccgaa catcttcatg aaggacagcc cgttggcgaa ggagaccaac
    16801 gtgatggtga gcaggatcgc agcgctactg atgattcgtc caacgcgccc aagcccgatg
    16861 acgatggcgt cgttgttcga cagccccgag tcgcgggctt ccttgatccg gctgagcaag
    16921 aagatctcat agtccacgga caagctgaat gcgatgcagc agagcaacac caccatggac
    16981 aggttgagcg gcgctggagt gatgccgagt atcgacgcca agtgcccgtt ctgaaagatc
    17041 cagaccatcg cgccgagaac cgcgctcaac accagcagat tgagagcaag cgcctttatc
    17101 ggcaccacga cgctgcccgt gaacaggaac agcaaaacaa acgtcgacaa cgctatgagg
    17161 aggattgcca atggaagccg gtctgcgaca gcggctcggc tgtcgatgag cgtggcggtg
    17221 ggaccgccca cctggacctg gtggtcggcg atcttcgcac ggatatcacg caccagctct
    17281 tgagccgcat ccgactgcgc gcccactgac acatacacca acccgtaagc ggcgccggac
    17341 ttttcgctct cgggaatggg gccggtgatc agcttcccgc gctcatatct gccactgggc
    17401 acatcgaccc ggaccacgtc gtccatgagc gacaccgggg cggcaagctc gttgagcgct
    17461 tcggtgttgg tgcgcgccaa caacgtgacg gcctgcgaga tgtctaccgc gtagtcttcg
    17521 cgcagcgatt ccgccagtaa ccgcgcgttc gagtccgatg gcagcgcgcg ctcgtcgggc
    17581 gtggcgaact gcacgctcag gaaggggatg cccagcgcaa gcaacgccgc gacgaccggc
    17641 agggcacacc gcagcggcct gcgtgtcaca aaatgggcgc aacgtcgcca gaacaccgaa
    17701 tccgccgaaa gcggaacctt acggcgcacc acggcaagtg cgtcgatccg tgtgcccagc
    17761 agggccagga gcgcgggcac caccacgata gcgctgagcg ccgacacgat gacgaccgca
    17821 ctcgccgtaa ttccgaccga ccgcaggaag taggtgggga acaccagaag tccacccatg
    17881 gccagggtga ccgtggccgc gctgaaaatg atggttcgac ctgccgtcgc gacggtcgcg
    17941 acaatcgccg attcattgtc cctgccccgg tcgcgttcct cgcggaatcg cgacaccatc
    18001 aggaggccga agtcgatgga cagcccgagt ccgaatgcgg tcgctacggt gagcgcgtgc
    18061 accgatacgt ccgtgaagct agtcatgacc aacaacacca gcattgtcgt gacgatcgac
    18121 gtcaaaccga cggctagcgg caggaaggcc gccacgagac cgccaaagac gagcaccagc
    18181 acggcgagcg agatgggcag cgcgatgctc tcgctgatga ggagatcgtg attgaccctg
    18241 tcctcgatct cttcctgcac gccaagcggt ccggcgacac gcaccgagac cgcttgctca
    18301 tgtgtgggca gctcttcgat gatccggctc gcgacctttg cggcctcgtc ggtggtcccg
    18361 gcgacattca ccagaatcag ccctgaatgc ccgtcgcgac tgcgcagatc gccggccgga
    18421 tcctgttgga acgagccgat caccttcgcc gccgtctcgg tttcgacgag ttgctgcagc
    18481 cgatccgcgg ccgccgtgac atcaggactg tcgacggtgc cgtca
//
LOCUS       DASRJA010000075         6801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1629947,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000075 DASRJA010000000
VERSION     DASRJA010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629947"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..709
                     /locus_tag="VFC01_03430"
     CDS             146..709
                     /locus_tag="VFC01_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN78729.1"
                     /translation="MASPRARNATQTRADILAAARVRFGSDGYERTTLRAVAADVGVD
                     PALVIRYFGSKQELFAEAADFTIDLPDLTGVRPDDVADALLPSFFAVWEDDTTFVALL
                     RAAMTSPTAADTMRRIFATQVAPKLAAVAPDHPAQRAGLMGAFVIGLATTRYVLANPA
                     VADLGHDELIAWARPVIRQLLVGPAPA"
     gene            complement(808..966)
                     /locus_tag="VFC01_03435"
     CDS             complement(808..966)
                     /locus_tag="VFC01_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78730.1"
                     /translation="MTANTTTPTRGVAAGAAAQHDCADRGCLAAMLGVELGDATASNA
                     EEGSSSSW"
     gene            complement(1244..3238)
                     /locus_tag="VFC01_03440"
     CDS             complement(1244..3238)
                     /locus_tag="VFC01_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HZN78731.1"
                     /translation="MTTGGRVLTPAGWAWARGLRVTTPDVGAVARSLLAVLAVAAVGL
                     SASPSAAVWAAGAAAIAGAIAMQDSPAGRVPLVIVVSLQLGVAVFLGAVFASNNAVFI
                     GVVAVWCFAAGMQWALGSKPGMVAAASAALLVISPPVARSLTAVVFVTLSTVAAGCVQ
                     AALIAVWPPQRWRVQREALTRAYRTLAADARGVAADREASVGTAPLTWLREAFVDSPA
                     TQRPMAYHGGYRLPERITATLGALRGGDGAIAQVLSPAADALDAIANHNHTARRDAEY
                     ALARVDAAVAAATGPQAAAAQRFSQQLHEAFASRFGEFRRPDLIASLRATANVMRGQL
                     AWTSPVLRHAVRLSSATALGVAADRFGAVAHGHWIALTVLMVLRPETAHTYTRCVGRV
                     AGIAVGTAVASVLTLIWQPTGSSAAFVAVLFLTVTYGVTRFGYLAVSAALAAAVLFLL
                     DIDSAAAGAPIQDLLFAVVIGGGLAVVAHVVLPDHGLIRLHQRAGELLKSEIDYAAAV
                     VKAFVHELDHPTEALSAAWQRAFRARAAFEAASGATRADSRELRRWLRSYRAALNAVT
                     SACTSLETSLSNEPTTALTPEFVAAVDDYVDALRGAPPSAATPWTVDIDELTAANQHV
                     REQGAGLAADNAAARVLVAEVGKITRSLADIAATGDLAAH"
     gene            3527..4363
                     /locus_tag="VFC01_03445"
     CDS             3527..4363
                     /locus_tag="VFC01_03445"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HZN78732.1"
                     /translation="MSTAAERAAQLDLVARLKSAYPELPDAPTPDMLDHDRITAYLKP
                     VHDVGGEPDAPMKYENKQYELWEHMTYVICEVLAWRGIWLSEERRRIGNVDVGRAEYL
                     GLPYYGRWLIAVARVLVEKHHIGLTELSERMAEVQARYEGGLKGKKLEAKPKSEGDGS
                     DVKRNEHHKRAVGKGDPGVYAGQAGKAKFKVGDAVVVRELPVIFYTRTPEYVRGAKGE
                     IAAVAYESPAAEDETWDRPATPEWFYVVRFNMAELWDGYTGTTDDVVQTEIPEHWLEA
                     AK"
     gene            4385..5092
                     /gene="scnC"
                     /locus_tag="VFC01_03450"
     CDS             4385..5092
                     /gene="scnC"
                     /locus_tag="VFC01_03450"
                     /EC_number="3.5.5.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiocyanate hydrolase subunit gamma"
                     /protein_id="HZN78733.1"
                     /translation="MAGHDHDHDRTVKPMVDEITDFEVLEIALRELCIEKGIFTAEEH
                     RRFTEFAEQIGPTPAAHLVAKAWVDPEFKKLALAEPMTASKEVGVDWMEPTGFGTPSD
                     FTAFQILADTPTVHNVIVCALCSCYPRPILGNSPEWYRTPNYRRRLVRWPRQVLAEFG
                     LYLPDEISIHVQDSNQKHRFMVMALQPQGTEDWTEDQLVEIVTRDVLIGVALPKAGVT
                     TNVITETRPAIHPGTAG"
     gene            5089..5430
                     /locus_tag="VFC01_03455"
     CDS             5089..5430
                     /locus_tag="VFC01_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiocyanate hydrolase"
                     /protein_id="HZN78734.1"
                     /translation="MSERIEALQEMVERNQVWPVMAAKYGVENPVPPWKTSLDGLCDA
                     LDHAACDENVPNFKERRDEEDALSATVYANLPYPENQLVALAHSLVARGVIDEAELKR
                     RLASVRERLEA"
     gene            5440..6150
                     /locus_tag="VFC01_03460"
     CDS             5440..6150
                     /locus_tag="VFC01_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78735.1"
                     /translation="MEKVIITLRGADPNETWCSRLRNDVAADILDTAVPGLAINVRDD
                     AVRDSLMTLTTLDPPVVGFATLWTQQSYGDQVRSAITRLEKECDDVAAYLVTESVPLP
                     PPPTQPGERTPGLANVALLRRPGDLDEAMWRQRWHIDHTPVAIETQSTFGYTQNEVVH
                     PLTSGAPVLNAIVEELFPIEATSDLHAFFGAADDDDLRDRMERMVASTTAFGANRDID
                     TVPTSRYVFRTPFTEVVP"
     gene            6147..6590
                     /locus_tag="VFC01_03465"
     CDS             6147..6590
                     /locus_tag="VFC01_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HZN78736.1"
                     /translation="MTTLDEAVALASDESGLAVVSTVRADGTIQASLVNAGKLPHPAS
                     GAEALAFVTYGKVKLANLRARPQLAATFRKGWMWATVEGRAELAGPDDPQPWLTDSEQ
                     LRLLLREVFTAAGGTHDNWDEYDRVMAEQRRTVVFVAPTRVYSNG"
     gene            6607..>6801
                     /locus_tag="VFC01_03470"
     CDS             6607..>6801
                     /locus_tag="VFC01_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN78737.1"
                     /translation="MTLLISDDNRVRTLTLNRPDVLNAFNEELYHATAVALRDTAADP
                     DVAVVLLTGAGRAFSAGNDLN"
BASE COUNT         1246 a         2268 c         2218 g         1069 t
ORIGIN      
        1 gcggcggtca ggccggtcgg ccctgcgccg acgatcagaa cgggcagatt catggtcggc
       61 tcctgtccct aagtcaacga ctggtgactt agacgatagg cctgggtgct ggtgttgtca
      121 acggttggtg acttatcctc gtggaatggc atcgccgcgc gcacgcaacg ccacccagac
      181 ccgcgccgac atcctggccg ccgcacgcgt tcggttcggg tcggacggct atgaacgcac
      241 cacgctgcga gcggtcgcgg cggacgtcgg cgtcgacccc gctctggtca tccggtactt
      301 cggcagcaag caggagttgt tcgccgaagc agcggacttc acgattgatc tgccggatct
      361 gaccggtgtg cgtcccgacg acgtcgcaga tgcgttgctg cccagcttct ttgccgtctg
      421 ggaggacgac accacattcg tcgcgctctt gcgcgcagcg atgaccagtc cgaccgccgc
      481 ggacaccatg cgaaggatct tcgcgaccca ggtggctcca aagctcgccg ccgtcgcacc
      541 cgaccacccg gcccaacgcg ccggcctgat gggcgcgttc gtgatcgggt tggcaaccac
      601 ccgctacgtg ctggctaacc ctgccgtggc agatctcggc cacgacgagc tgatcgcctg
      661 ggcccgcccc gtgattcgac agctgctggt cggccccgcg cctgcgtgac tcggtcgcca
      721 ggcggttgcg gtacgccagc cagttttgct tcgggcgtgg ggcgtacgac gcgtttgctg
      781 gatgcccgca cttcagcggc gttctgatca ccagctcgat gaggaacctt cctcagcgtt
      841 gctggcagtg gcgtcaccca gctcgacccc gagcatcgcg gccaggcagc cgcgatccgc
      901 gcagtcatgt tgcgcggccg ctccggcggc cactccacgg gtcggggtgg tggtgttggc
      961 agtcatctca attctcctgt ttgcgttgtc attccgaacg ttcaccgatt tgttccggcg
     1021 accacaagct atgtgtccgc agtcgatgtg gctcgcttgt gcgcgcaaac cgcacaagac
     1081 gcgatttacg ccttttgcgc attgtgctca aggagccgac gggggcgctg agccgacagc
     1141 ggacacaacg gcgcgggtat cgatgacgag cccggcgtgg tcgtcaatgg cgttgttgac
     1201 gatcaccggc agcgacacac cagaaaaatg tcggaggcag ctgctagtgc gcagcaaggt
     1261 cgccagtcgc cgcgatgtcc gccaggctcc tggtgatctt gccgacttcg gcgacgagta
     1321 cccgcgccgc cgcgttgtcg gcagcgagcc ctgccccttg ctcgcgtaca tgttgattcg
     1381 ccgcggtcag ttcatcgatg tcgactgtcc acggcgtggc cgcgctgggc ggtgcacccc
     1441 gcagggcatc gacgtaatcg tcgacagcgg cgacgaattc gggggtcagc gccgtcgtcg
     1501 gttcattgga caggctggtc tccaacgacg tgcatgcgct ggtgacggcg ttcagcgccg
     1561 ctcgatacga cctcagccac cgtcgcagct cgcgtgaatc cgcccgcgtc gcaccggacg
     1621 ccgcctcaaa cgcggcgcgg gcccggaagg ctcgctgcca cgccgccgac aacgcttcgg
     1681 tggggtgatc gagctcgtgc acgaacgcct tgacgaccgc tgccgcataa tcgatttcag
     1741 atttcagcag ctccccggcg cgctggtgca gccgaatcaa cccgtgatcc ggcagcacga
     1801 catgggccac cactgccaga cctccgccga tgacgaccgc gaacaacagg tcttgtatgg
     1861 gcgcgccagc cgcggcggaa tcgatgtcga gcaggaagag aacggccgcc gccaacgcgg
     1921 cgctgaccgc gaggtatccg aagcgcgtga ccccgtacgt caccgtaaga aatagcactg
     1981 ccacgaaggc ggccgacgaa cccgtcggct gccatatcaa agtcagcacc gacgcgaccg
     2041 cggttccgac cgcgataccc gcgacccgcc cgacgcagcg tgtgtaggtg tgtgcggtct
     2101 ccggccgcag caccatcaag acggtcaacg cgatccaatg accgtgcgcc acggcaccga
     2161 atcggtctgc ggccacaccc agcgcggtgg ccgacgacag ccgaacggcg tgccgaagca
     2221 ctggggacgt ccacgcgagt tgaccgcgca tcacattggc ggttgcccgc agcgaggcga
     2281 tcaagtcggg gcggcggaac tcaccgaatc gcgacgcgaa ggcctcgtga agttgctgcg
     2341 aaaaccgttg cgcggcggcc gcttgcggcc ccgtcgccgc cgctaccgca gcatcgacgc
     2401 gggccagcgc atattcagcg tcccgccgcg cggtgtggtt gtggttcgcg atcgcgtcga
     2461 gggcatcagc ggcgggggag agcacctgcg cgatcgcgcc gtcgccacca cgcagggcac
     2521 cgagcgttgc ggtgatccgt tcgggaagcc ggtagccgcc gtgatacgcc atcgggcgtt
     2581 gtgtcgccgg gctgtcgacg aacgcttcgc gcagccacgt gagtggggcg gtgccgaccg
     2641 acgcctcacg gtcggcggcg actccgcggg cgtcggcggc gagtgtccgg taggcgcgcg
     2701 tcagtgcttc gcgctgcacc cgccagcgtt gcggcggcca aacagcgatc agcgccgcct
     2761 gaacgcagcc ggcggccacg gtcgacagcg tcacaaacac caccgcggtc agcgaccgcg
     2821 ccaccggcgg ggagatcacc agcagcgcag cgctggccgc ggcgaccatg cccggcttac
     2881 tgccgagcgc ccactgcatg cctgccgcga agcaccacac cgcgacgacg ccgatgaata
     2941 ccgcgttgtt ggacgcgaac accgcgccga ggaacaccgc cacacccagt tgcagggaca
     3001 ccacgatgac cagcggcacc cgtccagcgg ggctgtcctg catcgcgatt gcgccggcaa
     3061 tcgccgccgc gccagcggcc cacaccgcgg ccgacggaga cgccgacagc ccgaccgcgg
     3121 ccaccgccag caccgcgagc aggctgcggg ccaccgcgcc gacgtccggc gtcgtcaccc
     3181 gcaggcctcg ggcccacgcc cagcccgccg gggtgagcac ccgaccgccc gttgtcacct
     3241 gcctattctg actccaccga gggcaccgaa cggtcaagca cacacgtcga atcctgtctc
     3301 gatggcggct ccgctcacat tgctgtacca gccagcaaag gctcaggcga ctacgaatgt
     3361 tgcgatcaaa acttcgccgc cgtcaaatgc tttgcggcac agcgcatgct ttcgacaagc
     3421 ggaattgacc acgctgaccg ggctattcga ggtaccgtcg tcgccaacgg cgttgctggc
     3481 tttcgtcgga tgtcaccctc ctgtccgaaa agcgaaggga gcttgcatga gtacggccgc
     3541 cgagcgtgca gcacagctgg atctcgtcgc tcgactgaag tcggcatatc cggagcttcc
     3601 ggacgcgccg acgcccgaca tgttggacca cgatcgcatc accgcgtacc tgaagccggt
     3661 gcatgacgtc ggcggtgagc cggacgcgcc gatgaaatac gaaaacaagc aatacgagct
     3721 ctgggagcac atgacctatg tgatctgcga ggtgctcgcg tggcggggga tctggctgtc
     3781 ggaggagcgc cgccggatag gcaacgtcga cgtcgggcgg gccgagtacc tggggttgcc
     3841 gtattacggc cgctggctga tcgctgtcgc gcgtgttctg gtggagaagc accacatcgg
     3901 tctgacggag ctgagcgagc gcatggccga ggtccaggcg cgttacgaag gcggcctgaa
     3961 gggcaagaag ttggaggcca aaccgaagtc cgaaggcgac gggtcggacg tcaaacgcaa
     4021 cgagcaccac aagcgcgcag tcggtaaggg cgatcccggg gtgtacgccg gtcaggccgg
     4081 gaaggcgaag ttcaaggtcg gcgacgcggt ggtggtgcgg gagcttccag tgatcttcta
     4141 cacccgcacc ccggagtacg tgcgaggcgc gaaaggcgaa atcgctgcgg tggcctacga
     4201 aagcccggcc gcggaggacg agacctggga tcgccctgcg acaccggaat ggttctacgt
     4261 cgttcggttc aacatggccg agttgtggga cggctacacc ggaaccaccg atgacgtcgt
     4321 ccaaaccgaa atccccgaac attggctgga agccgccaaa taagccgccc ggaaaggatt
     4381 ttgcatggct ggtcacgatc acgaccacga ccgcaccgtc aagccgatgg tcgacgagat
     4441 caccgacttc gaagtactcg aaatcgccct gcgcgaattg tgtatcgaga agggcatttt
     4501 caccgcggag gagcaccgtc ggttcaccga gttcgccgag cagatcggcc cgacccccgc
     4561 agcgcatctg gtcgccaagg cgtgggtgga tcccgagttc aagaagctcg ccctcgccga
     4621 accgatgacg gcgagtaaag aggtcggtgt cgactggatg gagcccaccg gcttcggcac
     4681 ccccagcgac ttcaccgcgt tccagatcct cgcggacacg ccgacggtgc acaacgtaat
     4741 agtctgcgcg ctgtgctcgt gctacccgcg gccaatcctc ggcaactcgc ccgagtggta
     4801 tcgcacgccc aactaccgca ggcgattggt gcgctggcca cgccaggtgc tggccgagtt
     4861 cggcctctac ctgcctgacg aaatctccat ccacgtacag gattccaacc agaagcatcg
     4921 cttcatggtg atggcgttgc agccgcaggg cactgaggac tggactgaag atcaactggt
     4981 ggagatcgtc acgcgtgatg ttctgatcgg cgtcgcactg ccgaaggcag gcgtcaccac
     5041 caacgtgatc accgagaccc gccctgccat ccaccccggg acagccggat gagcgagcgc
     5101 atcgaggccc tgcaggagat ggtcgagcgc aatcaggtct ggccggtgat ggccgcgaag
     5161 tacggcgtcg agaatcccgt gccgccgtgg aagaccagcc ttgacggcct gtgcgacgcc
     5221 ctcgaccacg ccgcgtgcga cgagaacgtc cccaatttca aagagcggcg cgacgaggaa
     5281 gatgcgttgt cggcaaccgt gtacgcgaac ttgccctacc cggagaatca gctcgtcgcg
     5341 ctggcgcact ccctggtggc tcgcggcgtc atcgacgagg cagaactgaa acgacgactc
     5401 gccagcgtcc gcgaaaggct cgaagcctag attcaagtcg tggaaaaagt catcatcacc
     5461 ctgcgcggcg ccgatccgaa cgaaacgtgg tgcagccgac tgcgcaacga cgttgccgcc
     5521 gacattctcg acacggctgt gcccggcctc gcgatcaatg tccgcgacga cgcggtgcgt
     5581 gactcactga tgacactgac gacgctggat ccaccggtgg tcgggttcgc gacgctgtgg
     5641 acgcagcagt cctacggcga tcaagtgcgt tcggcgataa cccggctgga aaaggagtgc
     5701 gacgacgtgg cggcgtatct ggtgaccgaa tcggtgccgt tgccaccacc accaacacaa
     5761 ccgggcgagc gcacgcccgg gttagcgaac gtcgcgctgc tgcgccggcc cggcgatctg
     5821 gacgaggcca tgtggcggca gcgctggcac atcgaccaca caccggtagc cattgagacc
     5881 cagtcgacgt tcgggtacac gcagaatgaa gtagtgcacc cgctgacgtc aggggcacca
     5941 gtactcaatg cgatcgtcga ggaactcttc ccgatcgagg cgacatccga cctccacgcg
     6001 ttctttggcg ccgccgatga cgacgatctg cgcgaccgga tggaacgcat ggtggccagc
     6061 accacggcgt tcggcgcgaa tcgagacatt gacacggtgc cgacgagtcg ctacgtattc
     6121 cggacaccgt tcacggaggt ggtcccatga cgacgctcga tgaagctgtt gcgctggcct
     6181 ccgacgaaag tggcctcgcg gtggtctcga cggtgcgcgc cgatggcacc atccaggcct
     6241 cgctcgtcaa cgcagggaag ctgccgcatc ccgcctccgg agcggaagcc cttgcgttcg
     6301 tcacctacgg caaggtgaag ctggcgaatc tgcgggcgcg gccccagctc gcggcgacct
     6361 tccggaaggg gtggatgtgg gcgacggtcg agggccgtgc cgagctcgcc ggacccgacg
     6421 atccgcagcc ctggctcacc gactccgagc aattgcgact actgctgcgc gaggtgttca
     6481 ccgccgcggg cgggacacac gacaactggg atgaatacga ccgggtgatg gccgagcagc
     6541 ggcgcaccgt ggtcttcgtc gcacccaccc gggtgtacag caacggttag cgaatacgct
     6601 gctggcgtga ctctcctcat cagcgacgac aaccgggtgc gcacgcttac cctcaaccgg
     6661 cccgacgtcc tcaacgcgtt caacgaggag ctgtaccacg cgaccgcggt cgcgctgcgt
     6721 gatacggcgg ccgatcctga cgtggcggtc gtgttgctga ccggtgccgg gcgggcgttt
     6781 tccgcgggca acgacctcaa c
//
LOCUS       DASRJA010000076        22710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3879998,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000076 DASRJA010000000
VERSION     DASRJA010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22710
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3879998"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2097
                     /gene="gcvP"
                     /locus_tag="VFC01_03475"
     CDS             <1..2097
                     /gene="gcvP"
                     /locus_tag="VFC01_03475"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HZN78738.1"
                     /translation="VVAADLLALTLLESPGSMGADVVVGSSQRFGVPLFYGGPHAGFM
                     SVRAGLERHLPGRLVGVSVDAEGRPAYRLALQTREQHIRRDKATSNICTAQVLLAVIA
                     AMYASYHGADGLAGIARRVHARAEAIAGALGDALVHDTFFDTVLARVPGRADEVIAAA
                     KATGINLWRVDADHVSVSCDEATTDEHVATVIEAFGLASAEPLRSDIATRTSEFLTHP
                     AFTRYRTETEMMRYLRSLADKDIGLDRSMIPLGSCTMKLNAAAEMEPITWPEFARQHP
                     FAPVSDTPGLRKLIADLESWLTAITGYDAVSLQPNAGSQGEYAGLLAIQAYHAERGQP
                     DRDVCLIPSSAHGTNAASAALVGMRVVVVACRANGDVDLDDLRAKVAEHADRLSALMI
                     TYPSTHGVFEHDIAEICAAVHDVGGQVYVDGANLNALVGLARPGRFGGDVSHLNLHKT
                     FCIPHGGGGPGVGPVAVRGHLAKYLPGHPLAEELPDKYTVSSAPYGSASILPITWAYI
                     RMMGAAGLRVASLTAIASANYIARRLDEYYPVLYTGENGMVAHECILDLRGITKETGV
                     TVDDVAKRLADYGFHAPTMSFPVAGTLMVEPTESESLAEVDAFCDAMIAIRDEIDQVG
                     AGTWPVDDNPLRGAPHTAECLLVADWDHPYTREQAAYPLGKGFRPKVWPPVRRIDGAY
                     GDRNLVCSCPPVEAFA"
     gene            2107..2982
                     /locus_tag="VFC01_03480"
     CDS             2107..2982
                     /locus_tag="VFC01_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN78739.1"
                     /translation="MLPPSVSGWRDGGTWLTTRAGRVFVRNADGDGPTILLLHGYPSS
                     SFDYRHAVPHLAGRAWLTLDFLGFGLSDKPRPHRYSLLEQADLVQAVVAEFAPGPVVL
                     VAHDMGTSVTTELLARDLQGHLPFDLQRVVLSNGSVILRRASLRPIQKVLRGPLGPLA
                     ARLSSKAAFTREFGRLFSRNHPLAAAEADAQWALMTNNDGHRIAHLLISYLDERERYA
                     ERWHGAVRDWPKPLSFLWALDDPVATTNVLAGLRELRPAADVVELPGVGHYPQVEVPE
                     VFTRAALSLLSTASH"
     gene            complement(2979..5147)
                     /locus_tag="VFC01_03485"
     CDS             complement(2979..5147)
                     /locus_tag="VFC01_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78740.1"
                     /translation="MGRHSAPDPEDAAGKDVPEEPPTQRFGRQRPEEPDHGPGYGEQD
                     YRGQGYRGYDRPDYREPAYPPTDYPEGDYDEPGYDEAGYGEPGYDEPGYGDAEYDESD
                     YPHPDYPELDEDYAQPEARRGGGATPPPRSTGPQHGGQWEGGEWTGSHRAVQTGRRGV
                     SIGVIAALVAVVVVVGAFILWRFFGDALSHRTDALASRCVDGELAVAVVADPSIADQI
                     HKLADAYNQSAAPVADKCVKVGVRPADSDQVVNGFIGKWPADLGERPALWIPASSVSE
                     ARLEAAAGAQTVSDSRSLVTSPVLLAVRPQLKDALAQQNWGTLPGLQTNPTALDGLNL
                     PGWGPLRLSLPLSGDSDASYLAAEAVAAASAPAGQPASAGAGAVNTLVGGQPKLSDTK
                     AATAMDALLSATDPATAPVHAVVTTEQQLFQRAASMPDAKGKLASWLPPGPTAIADYP
                     TVLLSGNWLSQEQVSAASEFARFMRKSEQLAELSKAGFRAEGASPPKSDVTSFAPVSA
                     PLSVGDTAMRATLANALTAPVGSPAVTIMLDQSMPTDEGGKSRLANVVAALNARLQAL
                     PANSAVGLWTFDGVEGRSEVSIGPLSDPVNGQPRSAALTSALNDQSASGGGAVSFTTL
                     RILYDQTKANFRQGQSNSILVITAGPHTDQSLDGSGLQQYIRGAFDQARPIAVNVIDF
                     GNDSDRATWEAVAQATGGSYQNLTNSTSPDLTTAITQFLG"
     gene            complement(5186..7378)
                     /locus_tag="VFC01_03490"
     CDS             complement(5186..7378)
                     /locus_tag="VFC01_03490"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HZN78741.1"
                     /translation="MTNRVTVGNLRVAQVLYDFINNEALPGTDIDPDSFWAGVDKVVT
                     DLSPQNQDLLDRRDQLQAQIDKYHRQHVIEPLDPEGYKQFLTEIGYLQPEPDDFTITT
                     AGVDDEITTTAGPQLVVPILNARFALNAANARWGSLYDALYGTDVISEDDGAEKGASY
                     NKVRGDKVIAYARKFLDGAVPLASGSWSEATGLKIDDGQLLIDLGDDLSTGLATPEQF
                     VGYTGELGSPQWSVLLVNNGLHIEVLVDPDSPVGSTDDAGIKDVVLESAITTIQDFED
                     SVAAVDAEDKVLGYRNWLGLNKGDLAEEVTKDGETFTRVLNQDRSYTTPDGGELTLPG
                     RSLLFVRNVGHLMTNDAIVDADGKEVFEGIQDALFTSLIAVHGLKQGDGNGPLLNSRT
                     GSIYIVKPKMHGPDEVAYTVELFSRVEDVLGLPQNTLKVGIMDEERRTTLNLKACIKA
                     AADRVVFINTGFLDRTGDEIHTSMQAGPMIRKGAMKSTQWIAAYEDQNVDIGLVTGFS
                     GRAQIGKGMWAMTDLMADMVEQKIGQPKAGATTAWVPSPTAATLHAMHYHEVDVYAVH
                     KELEGKRRTNIDQLLTVPLAKELAWAPEEIREEVDNNCQSILGYVVRWIDAGVGCSKV
                     PDIHNVALMEDRATLRISSQLLANWLRHGVITEEDVKASLRRMAAVVDKQNEKDPEFR
                     PMAPDPDGSIAFQAAQELILSGGAQPNGYTEPILHRRRREYKAASNGG"
     gene            complement(7462..8340)
                     /locus_tag="VFC01_03495"
     CDS             complement(7462..8340)
                     /locus_tag="VFC01_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase"
                     /protein_id="HZN78742.1"
                     /translation="MPDLVQLDEPDWTTRERIHQKRVDEFCEPHRRRARAGQVHPVWD
                     FLFTYYSLRPRQLRVWHPGFGVTLGGAAAERYLDRSGYGRGRDGVSVTGEHLLARVDT
                     VRFISRLLRATAARPARLNCFGLHEWAMVYRASAVRHGQVPLRLGAAGTDAVVESMPL
                     RCSHFDAYRFFTKPAMSRNTEELTRDGQVMAEQPGCVHAGMDLYKWAFKLGPLVPSDL
                     VMDCLELAADARSLDMRASPYDLRDYGFEPIAVETPAGRAEYVRSQHSIAERAAPLRA
                     NLADRCDLLLSAAGGE"
     gene            complement(8333..9385)
                     /locus_tag="VFC01_03500"
     CDS             complement(8333..9385)
                     /locus_tag="VFC01_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZN78743.1"
                     /translation="MGDLFGVILTALLLAANAFFVGAEFALISARRDRLEALAEQGKH
                     SAVTVIRAGENLPLMLAGAQLGITICSILLGRVGEPAVAHLLEKPFHLLGIPDTILHT
                     VSFVIAITIVVVLHVLLGEMVPKNIAIAGPESSAMLLVPPYLLWVRAARPVIAVYDWC
                     AHVILRAFGVEAKSELENTVSTLELSEMIAESLSEGLLDPEEHNRLSRALRIRNRVVA
                     DVAMPLADVHAVPVAADEYGPTVAAIRSALAETGYSRFPVVDSWGRFIGYVHIKDVLP
                     AVDDPDAVVDLTTVRPLPEVAETTPLPDALSLLRRENSHLALVTATDGGVSAMVTLED
                     LVEDVVGAVRDRTHRA"
     gene            complement(9378..10742)
                     /locus_tag="VFC01_03505"
     CDS             complement(9378..10742)
                     /locus_tag="VFC01_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZN78744.1"
                     /translation="MSAMFTVLAVLAFLVLTAGTAVFVSAEFSLTALERSTVDANARG
                     GGRRDQLVQRAHRTLSFQLSGAQVGISITTLITGYLAEPLVARLLEPLFAAMSLSHDV
                     SSTLALVLALLIATSVSMVFGELVAQYLAVAKPLPVARVVAGPQLLFSMLFTPLIHLT
                     NGTANWILRRLGIEPAEELRSARSPQELGSLVRNSARRGSLDPVTATLVDRSLQFGSR
                     TAEELMTPRTEIVTLEVDDTIADLVAAAIESGYSRFPIVDGDLDETIGIVHVKQVFEV
                     PPEQHTKTRLATLAQPVARVPSTLDGDDVMAQLRANGLQTALVVDEYSGTAGMVTVED
                     VIEEIVGDIRDEHDEPTADVVQAGQGWHVSGLLRIDEVADATGFRASEGEYETIGGLV
                     LQELGHIPEEGESVELAAFDPDAPLEDPVRWLATVVRMDGRRIDQLELTQLGRREEEH
                     GNDG"
     gene            complement(10932..12368)
                     /locus_tag="VFC01_03510"
     CDS             complement(10932..12368)
                     /locus_tag="VFC01_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="HZN78745.1"
                     /translation="MRFLEGHDPAYDLTYNDVFIVPGRSDVQSRFDVDLSTHDGSGTT
                     IPVVVANMTAVAGRRMAETIARRGGIVVLPQDLPISAVKNTVDFVKSRDLVVDTPVVL
                     SPDDSVSDAMALIHKRAHGTAVVVFEGRPIGLVTEADCVGVDRFTRVRDVAISDFITA
                     PAGTDPRKVFDLLEHALVDVAVLTEPDGTLAGVLTRTGAIRAGIYTPAVDDKGRLRIA
                     AAVGINGDVGAKARSLAEAGVDLLVIDTAHGHQLKMLDAIKSVSSLDLGVPLAAGNVV
                     SAEGTRDLITAGASIVKVGVGPGAMCTTRMMTGVGRPQFSAVVECAAAARQLGGHVWA
                     DGGVRHPRDVALALAAGAANVMIGSWFAGTYESPGDLMRDREDQPYKESYGMASKRAV
                     AARTAADSSFDRARKALFEEGISTSRMGLDPSRGGVEDLLDHITSGVRSTCTYVGAAN
                     IAELHDKVVLGVQSAAGFAEGHPLPTGW"
     gene            complement(12390..13850)
                     /gene="gndA"
                     /locus_tag="VFC01_03515"
     CDS             complement(12390..13850)
                     /gene="gndA"
                     /locus_tag="VFC01_03515"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HZN78746.1"
                     /translation="MSSSESPTNGTAQIGVTGLAVMGSNIARNFAHHGYTVALHNRSI
                     AKTDALLKDHGDEGKFVRSETIPEFLAALEKPRRVLIMVKAGDPTDAVINELADAMEE
                     GDIIIDGGNALYTDTIRREKAMRERGLHFVGAGISGGEEGALNGPSIMPGGPAESYKS
                     LGPLLEEISAHVDGVPCCTHIGPDGAGHFVKMVHNGIEYSDMQLIGEAYQLLRDGLGL
                     SAPQIADVFTEWNKGDLDSFLVEITAEVLRQVDARTGKPLVDVILDEAEQKGTGRWTV
                     KSALDLGVPVTGIAEAVFARALSGSVAQRHATTGLASGDLGEKPSGADKFVEDVRQAL
                     YASKIVAYAQGFNQIQAGSAEYDWGITPGDLATIWRGGCIIRAKFLNRIKEAFDEDPN
                     LPTLITAPYFRSAVENAIDSWRRVVVTATQLGIPIPGFSSALSYYDALRTERLPAALI
                     QGLRDFFGAHTYARIDDDRDKKFHTLWSGDHSEVPA"
     gene            complement(13900..14817)
                     /locus_tag="VFC01_03520"
     CDS             complement(13900..14817)
                     /locus_tag="VFC01_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZN78747.1"
                     /translation="MSALAFTIVALVLSGPVPAMLARATWPMRAPRAAIVLWQSIALA
                     AVLSAFSAGIAIASRLFVPGPDGRPTATITSEIDVLGWPVWIAYLLVFALTLVIGARL
                     TIAVLQVAIATRRRRAHHRMVVDLVGKPQGGLRILDVKEPLAYCLPGVRSRVVVSQGT
                     LTTLSDNEIAAILSHERAHLRARHDLVLEMFTAVHAAFPRFVRSGNALDAVRLLIEML
                     ADDAAVRAAGPTPLARALVACAAGRTPSGALAVGGPTTVLRVRRLGGRPNSRAFAATA
                     YLAAAAVLVVPTLALAVPWLTELHRLFTA"
     gene            complement(14832..15248)
                     /locus_tag="VFC01_03525"
     CDS             complement(14832..15248)
                     /locus_tag="VFC01_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HZN78748.1"
                     /translation="MAKLTRLGDLERAVMDHLWSSREPQTVRQVHEALSARRDLAYTT
                     IMTVLQRLAKKNLVVQHRDDRAHRYAPTHGRDELVAGLMVDALDQVSDTGSRQAALVH
                     FVERVGADEAAALRRALAELESKNTEAPPAGNPGTG"
     gene            15467..16225
                     /locus_tag="VFC01_03530"
     CDS             15467..16225
                     /locus_tag="VFC01_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron reductase"
                     /protein_id="HZN78749.1"
                     /translation="MTVLIEDPLIAGMAIRRTLPLHESSRRLRELHPECPRVYGVAVM
                     GDLSRRRWWPLADAVTTDRLQTMFDIGAAETDSRASIAQQLAATLAHVVVGRVVPLLV
                     LEGRAWDTGLENLWVHVDSEGTIDWVGVVDPQLRALPDDPALADGVPSAGIVALPSEA
                     ALTTWVAHRSHRALDPLFARLVEVCAGVMSMASMWHIVGAAVVSAATHVPLLAGSSEV
                     TSMRRAQAVLDALVGFGLPVRGATRIGARKALLN"
     gene            complement(16357..16734)
                     /locus_tag="VFC01_03535"
     CDS             complement(16357..16734)
                     /locus_tag="VFC01_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZN78750.1"
                     /translation="MAKTAKDLVAAANDVVPKISPAQAQELIAKGNALVVDVRDAPEV
                     EQSGKVAGAVHVSRGMLEFRADPESPYHDAQFAKDKTVILYCASGGRSALSGKALKEL
                     GYAEVYNMGAFKDWAESGGAIDK"
     gene            16796..17194
                     /locus_tag="VFC01_03540"
     CDS             16796..17194
                     /locus_tag="VFC01_03540"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN78751.1"
                     /translation="MTTEGEFGSGFDNWLGLTYIEMTPDGGRARLEIDDKLLQPWGIV
                     HGGVYCSIIESLASVSAHVWLSENGGGTVVGVNNNTDFLRAISAGTVTAVSTPIHRGR
                     RQQLWLITITDDNDRVIARGQVRLQNIPPE"
     gene            17249..17551
                     /locus_tag="VFC01_03545"
     CDS             17249..17551
                     /locus_tag="VFC01_03545"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HZN78752.1"
                     /translation="MRLTPHEQDRLLISYAAELARRRQARGLRLNHPEAVALITDHLL
                     EGARDGRTVAELMVSGREVLSRDDVMDGVPEMLYDVQVEATFPDGTKLVTVHQPIS"
     gene            17548..17862
                     /locus_tag="VFC01_03550"
     CDS             17548..17862
                     /locus_tag="VFC01_03550"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HZN78753.1"
                     /translation="MIPGEIVYGDGDIEINPGAARLTLDVVNTGDRPVQVGSHVHLPQ
                     ANASLQFDRDAAHGHRLDIPAGTAVRFEPGVAQHVSLVPLSGSREVHGLSLNPPGRLD
                     AS"
     gene            17859..19580
                     /locus_tag="VFC01_03555"
     CDS             17859..19580
                     /locus_tag="VFC01_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HZN78754.1"
                     /translation="MTELSRARYAALFGPTTGDRIRLADTDLFVEITEDRSGGPGLAG
                     DEAVFGGGKVLRESMGQGRATRADGAPDTVITGVVIIDHWGIIKADIGIRDGRIAAIG
                     KAGNPDIMTGVHPDLVVGPSTEIISGNGRIVTAGAIDCHVHLICPQIMEEALGGGITT
                     IIAGGTGPAEGSKATTVTPGSWHLARMFESLDSWPLNIALLGKGNTVSAEAMWEQLRG
                     GAAGFKLHEDWGTTPAAIDACLTVSDAAGVQCNIHTDTLNEAGFVEDTLAAVKGRSIH
                     AYHTEGAGGGHAPDIITVVSHPNVLPSSTNPTRPHTLNTLDEHLDMLMVCHHLNPSVP
                     EDLAFAESRIRPSTIAAEDLLHDIGAISMIGSDAQAMGRIGEVVLRTWQTAHVMKRRR
                     GALEGDSKADNNRARRYVAKYTICPAIAHGLDHEIGSVEVGKLADLVLWEPAFFGVRP
                     HAVLKGGMIAWAAMGDANASIPTPQPVLPRPMFGAAPAAAAATSVHFVSQQAIEDGLA
                     DRIDVKRRLAAVKDVRKVGKADMPLNDALPHIEVDPDTFTVRIDGDVWQEQPAAELPM
                     AQRYFLF"
     gene            19580..20215
                     /locus_tag="VFC01_03560"
     CDS             19580..20215
                     /locus_tag="VFC01_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HZN78755.1"
                     /translation="MTNLSTLLALADSRLPTGGHVHSGGVEEAVTGGLVADLTTLRAY
                     LWRRIRTHGLVAASLAAAVHAGSLTIEEADRETDARTPAPAARQSSRAQGRGLARLAR
                     RVWADHDWQPLGTTPHLAVAAGAVGKASGLNPDQTALSVVYTTMTGSATAAQRLLALD
                     PADVAALTFELAPLCEATAAEATKELADLSDPLLDVLAQRHSQRERPLFVS"
     gene            20226..20903
                     /gene="ureG"
                     /locus_tag="VFC01_03565"
     CDS             20226..20903
                     /gene="ureG"
                     /locus_tag="VFC01_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HZN78756.1"
                     /translation="MPPHYLDGEPHAHTERPRRQRQLGEPLRIGVGGPVGSGKTALVA
                     ALCRQLRDDLSLAVLTNDIYTTEDADFLRRHAVLADERIAAVQTGGCPHTAIRDDITA
                     NLDAIDDLIAHNDRLDLILVESGGDNLTATFSSGLVDVQIFVVDVAGGDKVPRKGGPG
                     VTFSDLLVVNKTDLAPLVGADLEVMRRDAADVRGDRPTVLISLTEDPAATAVLAWVRE
                     QLCVPTS"
     gene            20885..21511
                     /locus_tag="VFC01_03570"
     CDS             20885..21511
                     /locus_tag="VFC01_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HZN78757.1"
                     /translation="MRSDVLIVAHRDRQPRIECGGGVAARRTEPDTVHLVSAAATPLG
                     GDAIHIRVVVEPGAQLRVRTAAATVTLPGSATVESHASWTLEVAGDLDLDPQPTVVAA
                     TSRHFTSTRLELTGAGRVRLRERIQIGRSDERQGFWSGSLHADLDGSPLLRHGIELGN
                     GSVADDALGTPMACVSEFHYPDSDVETQGVALALAGGGCLSTWQGDRL"
     gene            complement(21508..>22710)
                     /locus_tag="VFC01_03575"
     CDS             complement(21508..>22710)
                     /locus_tag="VFC01_03575"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN78758.1"
                     /translation="TGIISSGEIAPPTRIILRKQKNAQVLLGNVTHIDLANKTVKSEL
                     LGHTYVTPYDSLIIAAGAGQSYFGNDHFAEWAPGMKTVDDALELRGRILGAFEQAERS
                     SDPARREKLLTFTVVGAGPTGVEMAGQIAELADHTLKGAFRHIDSTQARVILLDAAPA
                     VLPPMGEKLGLKARARLEKMGVEIQLGAMVTDVDRNGITVKDSDGTIRRIESATKVWS
                     AGVSASPLGRDLADQSDVELDRAGRVKVLPDLSLPGHRNVFVVGDMAAVEGVPGMAQG
                     AIQGAKYAAKAIKAELKGADPAQREPFQYFDKGSMATVSRFSAVAKIGPIEFGGFIAW
                     LAWLVLHLVYLVGFKTKVTTLLSWTVTFLSRARGNLTITEQQAYARTRIEELKEIAAG
                     VQDERAAS"
BASE COUNT         3606 a         7731 c         7605 g         3768 t
ORIGIN      
        1 gtggtcgccg ccgacctgct ggcgctcacg ctgctggagt cgccgggctc gatgggcgcc
       61 gacgtcgtcg tcggctcctc gcagcgcttc ggcgtcccgc tgttctacgg cggcccgcac
      121 gccgggttca tgtcggtgcg cgccggcctg gagcggcacc tgcccggccg cctcgtcggc
      181 gtctcggtgg acgcggaggg ccgcccggcg taccggctgg cgctgcagac ccgcgagcag
      241 cacatccgcc gggacaaggc caccagcaat atctgtaccg cgcaggtgct gctggcggtg
      301 atcgccgcga tgtacgccag ctaccacggc gccgacgggc tggccgggat cgcgcgccgg
      361 gtgcatgccc gtgcggaggc aatcgccggt gcgctcggcg atgcattggt gcacgacacg
      421 tttttcgaca ccgtgctggc ccgcgtgccc ggccgcgccg acgaggtgat cgcggcggcc
      481 aaggccaccg gcatcaacct gtggcgcgtc gacgccgacc acgtctcggt ctcgtgtgac
      541 gaggccacca ccgacgaaca cgtcgcaacg gtgatcgaag cgttcgggtt ggcctccgcc
      601 gagccgctgc gctcggacat cgccacccgc acctcggaat tcctcaccca tccggcgttc
      661 acccgatacc gcaccgaaac cgagatgatg cgctatctgc ggtcgctggc ggacaaggat
      721 attgggttgg accgcagcat gattccgctc gggtcgtgca cgatgaagct caacgcggcc
      781 gccgagatgg agccgatcac gtggccggag ttcgcgcgcc agcacccgtt cgcgcccgtg
      841 tcggacacgc cgggactgcg caagctgatc gccgacctgg agtcctggct gaccgcaatc
      901 accggctacg acgcggtctc gctgcagccg aacgcgggtt cgcaggggga gtacgcgggg
      961 ctgctggcga tccaggcgta tcacgccgag cgcggccagc ccgaccgcga cgtatgcctg
     1021 attccgtcca gcgcccacgg caccaacgcg gcgtcggcgg cgctggtcgg catgcgggtc
     1081 gtggtggtgg cgtgccgcgc caacggtgac gtcgacctcg acgacctgcg cgcgaaggtg
     1141 gccgagcacg cggatcggtt gtccgccttg atgatcactt acccgtcgac gcacggcgtt
     1201 ttcgaacatg acatcgccga gatctgtgcg gcggtacacg atgtcggcgg ccaggtgtac
     1261 gtcgacggcg ccaacctcaa cgcgctcgtc gggctggccc ggccgggccg gttcggcggc
     1321 gacgtcagcc acctcaatct gcacaagacg ttctgcatcc cgcacggggg cggcggccct
     1381 ggcgtgggcc cggtcgcggt gcgcgggcac ctcgcgaagt atctgcccgg tcatccgctg
     1441 gccgaggaac tgcccgacaa gtacacggtg tcgtcggcgc cgtacggctc ggcgtcgatc
     1501 ctgccgatca cctgggccta catcaggatg atgggcgcgg ccggcctgcg cgtggcgtcg
     1561 ctgactgcga tcgcgtcggc caactacatc gcccgtcgcc tcgacgagta ctacccggtg
     1621 ctgtacacgg gcgagaacgg catggtggcc cacgagtgca tcctggatct gcgtggcatc
     1681 accaaggaaa ccggtgtcac cgtcgacgat gtggcaaaac gcttggcgga ctacggcttc
     1741 cacgcaccga cgatgagctt cccggtggcc ggcacgttga tggtcgagcc gactgaaagc
     1801 gagagcctgg ccgaggtcga cgcgttctgc gacgcgatga tcgcgatccg cgacgagatc
     1861 gaccaggtcg gcgccggcac ctggcccgtc gacgacaatc cgctgcgcgg tgcgccgcat
     1921 acggcggagt gcctgctggt ggcggactgg gatcacccct acacccgcga gcaggcggcc
     1981 tacccgctgg gcaagggctt ccggcccaag gtctggcctc cggtgcgccg catcgacggc
     2041 gcgtacggag accgcaattt ggtctgctcg tgccctccgg tggaggcgtt cgcataatct
     2101 gacggcatgc tgcccccgag tgtgtctggg tggcgcgacg gcggcacatg gttgacgacg
     2161 cgtgcgggtc gcgtgttcgt ccgcaacgcg gacggcgacg gccccacgat cctgctgctg
     2221 cacggctatc cgtccagttc gttcgactac cggcacgcgg tcccgcacct cgcgggacgg
     2281 gcgtggctga cgcttgactt ccttggcttc ggcctttcgg acaaaccccg cccgcaccgc
     2341 tacagcctgc tcgaacaggc tgacctcgtg caggccgtcg tagccgagtt cgcgcccggt
     2401 ccggtggtgc ttgtcgcaca cgacatgggg acctcggtca ccaccgaact tctggcccga
     2461 gatctccagg gccacttgcc gtttgatctg cagcgcgtcg tgctcagcaa cggtagcgtc
     2521 atcctgcgcc gcgccagcct gcggcccatc cagaaggtgc tacgcggtcc gctcggtccg
     2581 ctcgcggcac ggctgtccag caaggccgcg tttacccggg agttcggccg gctcttcagc
     2641 cgaaaccacc cgctggcggc cgccgaggcc gatgcacagt gggccctgat gacgaacaac
     2701 gacgggcatc gcatcgcaca cctgctcatc tcctacctcg acgagcgcga gcgctacgcc
     2761 gagcgctggc acggcgcggt gcgggactgg cccaagccgc tgagcttcct gtgggcgctc
     2821 gacgatccgg tcgcaaccac gaatgtgctt gccgggttgc gtgaattgcg gcccgccgca
     2881 gacgttgtcg aattgccggg ggtcgggcat tacccgcaag tggaagtgcc ggaggtgttt
     2941 acccgcgcgg cactgagcct gctgtcaacg gcgtcgcatt aacccaggaa ctgcgtgatg
     3001 gccgtcgtca ggtcgggcga cgtcgagttg gtcaggttct ggtagctgcc gccggtggct
     3061 tgcgcgacgg cctcccaagt cgcccggtcc gagtcgttgc cgaagtcgat gacgttgacg
     3121 gcgatcgggc gcgcctgatc gaacgcaccg cgaatgtact gctgcagccc cgagccgtcg
     3181 agcgactggt ctgtatgcgg gcccgcggtg atgaccagga tcgaattgct ttggccctga
     3241 cggaaattcg ccttggtctg gtcgtacagg atccgcagcg tggtgaacga caccgcaccg
     3301 ccgcccgacg cgctctggtc gttcagcgca ctggtcaacg ccgccgaacg cggctgcccg
     3361 ttgacgggat cggacagcgg gccgatgctc acctcggagc ggccttcgac gccgtcgaac
     3421 gtccacaggc cgaccgcgga attcgcgggc aacgcctgca gccgcgcgtt gagcgccgct
     3481 accacgttgg cgagccggga cttgccgccc tcatcggtcg gcatcgactg gtcgagcatg
     3541 atcgtcacgg ccgggcttcc gaccggcgcg gtcaacgcat tggccagcgt cgcccgcatc
     3601 gcggtgtcgc ccaccgacag cggcgccgac accggcgcaa agctggtcac gtcgctcttc
     3661 ggcgggctgg cgccctcggc gcggaaaccg gccttggaca gttcggccag ctgctcggat
     3721 ttgcgcatga accgagcgaa ttcactggcc gcgctgacct gttcctgcga cagccaattg
     3781 ccgctgagca gcaccgtcgg ataatccgct atcgccgttg ggccgggcgg cagccatgat
     3841 gcgagcttgc ccttggcgtc gggcatcgac gcggcgcgct ggaacagctg ctgttcggtc
     3901 gtcaccacgg cgtgcaccgg agcggtggcc gggtcggtgg cgctaagtag tgcatccatc
     3961 gcggtggcgg ccttggtgtc ggacagcttg ggctgaccgc ccaccaacgt gttgactgcg
     4021 cccgcgccgg cgctggccgg ctgccccgcg ggcgccgacg ccgccgcgac ggcctcggcc
     4081 gccaggtagg aggcatcgct gtcaccgctg agcggtaacg acagccgcag cggaccccaa
     4141 cccgggaggt tgagaccgtc tagcgccgtc gggttggtct gcagacctgg cagcgttccc
     4201 caattctgtt gggccaacgc gtctttgagc tgcggccgaa cagcgagcag gacgggtgag
     4261 gtgaccagcg agcggctgtc gctgacggtc tgcgctcctg cggccgcctc gagccgggcc
     4321 tcggacaccg aactggccgg gatccacagt gcggggcgct caccgaggtc ggccggccat
     4381 ttgccgatga agccgttcac gacctggtcg gagtcggcgg gtcggacacc caccttgacg
     4441 cacttgtcgg cgacgggggc cgcgctctgg ttgtaggcgt cggccagctt gtgaatctga
     4501 tcggcgattg acgggtcggc gacgacggcg acggcgagtt caccgtccac gcagcgggag
     4561 gcgagcgcgt cggtgcgatg ggacagcgcg tcgccgaaga accgccacag gatgaacgcg
     4621 ccgaccacca ccacgaccgc gaccagtgcc gcgatcacgc caatgctgac gccgcgcctg
     4681 ccggtttgca ccgcgcggtg gctgcccgtc cactcgccgc cttcccattg gccgccgtgc
     4741 tgcggtcctg tcgaccgcgg cgggggagta gccccaccac cgcggcgggc ttccggctgc
     4801 gcgtagtcct cgtcgagctc ggggtagtcg gggtgcggat agtccgactc gtcgtattcg
     4861 gcgtcgccgt atcccggttc gtcgtagcct ggctcgccgt acccggcctc gtcgtacccg
     4921 ggctcgtcgt agtcgccctc gggatagtcg gtcggcgggt aggcgggttc ccggtagtcc
     4981 gggcggtcgt atccgcgata tccctggccg cgatagtcct gctcgccgta gcccgggccg
     5041 tgatcgggtt cctcgggccg ctgtcgaccg aaccgctgtg tcggcggctc ctcgggcacg
     5101 tccttgccgg ccgcgtcctc gggatcgggc gcgctatgcc tgcccactta caccctcgtg
     5161 ctcactgtgt cggccggtcg tcgcatcatc caccgttgct tgctgcctta tattcgcgtc
     5221 gccgccgatg caggatgggc tcggtgtagc cgttgggttg cgcgccgccg gacaggatca
     5281 gctcctgcgc ggcctggaac gcgatgctgc cgtcgggatc cggtgccatt ggccggaatt
     5341 ccggatcctt ctcgttctgc ttgtcgacga cagcggccat ccggcgcagg ctcgccttga
     5401 cgtcctcttc ggtgatcacg ccgtgtcgca accagttcgc cagcagctgg ctcgaaatgc
     5461 gcagtgtggc acggtcttcc atcaaggcga cgttgtggat gtcgggcacc ttggagcagc
     5521 cgacgcccgc gtcgatccag cgcaccacat agccgaggat cgactggcag ttgttgtcga
     5581 cctcttcgcg gatctcctcg ggcgcccacg ccagctcctt ggccagcgga accgtcagca
     5641 gctggtcaat gttggtgcgg cgcttgcctt ccagctcctt gtgcacggcg tacacgtcga
     5701 cctcgtggta gtgcatggcg tgcagggtgg ccgccgtcgg cgacggcacc cacgcggtcg
     5761 tagcaccggc cttcggttga ccgatcttct gctcgaccat gtcggccatc aggtcggtca
     5821 tcgcccacat gcccttgccg atctgggcgc gacccgaaaa accggtgaca agcccgatgt
     5881 cgacgttctg gtcctcgtag gctgcgatcc actgcgtgct cttcattgca cccttgcgga
     5941 tcatcggtcc ggcttgcatc gaggtgtgga tctcgtcgcc ggtgcggtcc aggaaaccgg
     6001 tattgatgaa caccacgcgg tcggcggccg ccttgatgca ggccttcagg ttgagggtgg
     6061 tgcggcgctc ctcgtccatg attccgacct tgagcgtgtt ctgcggcagc ccgagcacgt
     6121 cctcgacccg gctgaacagc tcgaccgtgt aggccacctc gtcggggccg tgcatcttcg
     6181 gcttcacgat gtagattgaa ccggtccggc tgttgagcag cggcccgttc ccgtcaccct
     6241 gcttcagccc gtgcacggcg atcaggctgg tgaacaaggc gtcctggatg ccttcgaaca
     6301 cctctttacc gtcggcgtcg acgatcgcgt cgttggtcat cagatggccg acattgcgga
     6361 cgaacagcag gctgcgcccc ggcagggtga gttctccgcc gtccggtgtg gtgtagctgc
     6421 ggtcctggtt gaggacacga gtgaaggtct cgccgtcctt ggtgacctcc tcggccaggt
     6481 cgcccttgtt caggcccagc cagttgcggt aaccgagcac cttgtcctcg gcgtcgacgg
     6541 cggccaccga gtcctcgaag tcctggatcg tggtgatcgc cgactccagc accacgtcct
     6601 tgatgccggc gtcgtccgtc gatccgaccg gggagtcggg gtcgacgagg acctcgatgt
     6661 gcaggccgtt gttgacaagc agcacagacc actggggcga gccgagctcg cctgtgtagc
     6721 cgacgaactg ctccggcgtg gccagcccgg tcgacaggtc gtcgccgaga tcgatcagca
     6781 actgaccgtc gtcgatcttc aacccagtag cctcgctcca cgaccctgat gccagcggaa
     6841 cggcgccgtc gaggaactta cgggcgtacg cgatgacctt gtcgccgcgc accttgttgt
     6901 agctggcgcc cttctcagcg ccgtcgtcct cggagatcac gtccgtgccg tacagcgcgt
     6961 cgtacagcga accccagcgg gcgttggcgg cgttcagcgc aaaccgcgcg ttgaggatcg
     7021 gcaccaccaa ctgcggaccc gcggtcgtgg tgatctcgtc gtcgacgccg gcggtggtga
     7081 tggtgaagtc atccggctcc ggttgcagat agccgatctc ggtgaggaac tgcttgtagc
     7141 cttccgggtc gagcggctcg atgacgtgct ggcggtgata cttgtcgatc tgcgcctgca
     7201 gctggtcgcg gcggtcgagc aggtcctggt tctgcgggct gagatcggtg acgaccttgt
     7261 cgacgcccgc ccagaagctg tctggatcga tatcggtgcc cggaagcgcc tcgttgttga
     7321 tgaagtcgta caacacctgg gcgacgcgca ggttccccac cgtcacgcga ttagtcatgc
     7381 gcgatccgcc ttcgctcctc gctcgctggt catttttcct ccctaaagcc gtcgatccag
     7441 cttacccagc ggtagctggg gctattcgcc accagccgca ctcagcagca ggtcacaccg
     7501 gtcggccagg ttggcccgca aaggcgcggc ccgctcagca atgctgtgct gggaacggac
     7561 gtactcggcg cgacccgccg gcgtctcgac ggcgatcggc tcgaatccgt agtcgcggag
     7621 atcatagggg ctggcccgca tatccagcga acgggcatcg gcggccagtt ccagacagtc
     7681 catcaccaga tccgacggca ccagcggacc gagcttgaac gcccacttgt acaaatccat
     7741 cccggcgtga acgcagcccg gttgttcggc catcacctgg ccgtcgcggg tgagctcttc
     7801 ggtgttgcgg ctcatggccg gcttggtgaa gaagcggtag gcgtcaaagt ggctgcagcg
     7861 caacggcatt gattccacga cggcgtcggt gcccgcggcg ccgagtcgca gcgggacctg
     7921 gccgtgtcgc accgccgacg cccggtacac catcgcccac tcgtgcagcc cgaagcagtt
     7981 gagccgtgcc ggacgggcgg ccgtggcacg cagcaaccgg gaaatgaacc gcaccgtatc
     8041 gacgcgcgcc aacaagtgct caccggtcac cgacacacca tcgcgaccgc gcccgtagcc
     8101 gctgcgatcg agataccgtt cggccgcagc gccgccgagc gtcacgccga agccggggtg
     8161 ccagacccgc aactgccgcg gccgcaggct gtagtaggtg aacaggaagt cccacacggg
     8221 atggacctgg cccgcccgcg cccgcctgcg atgtggctca cagaactcgt ccacccgttt
     8281 ctggtgtatc cgctcgcggg tggtccagtc gggctcgtcg agctgcacga gatcaggcac
     8341 ggtgcgtcct gtcacggacc gcgccgacga cgtcctccac caggtcttcc agcgtgacca
     8401 tcgccgacac accgccatcc gtggccgtga cgagcgcgag gtggctgttc tcccggcgca
     8461 gcagcgacag cgcgtcgggc agcggcgtcg tctcggccac ctctggcaac ggccgtaccg
     8521 tcgtcaggtc gaccaccgca tccgggtcat cgacagcggg cagcacgtcc ttgatgtgca
     8581 cgtagccgat gaaccggccc cacgaatcaa ccaccgggaa gcgggaatag ccggtctccg
     8641 ccagcgcgct ccggatcgca gccactgtcg gtccatactc gtcagcggcc accggcaccg
     8701 catgcacatc agccagtggc atcgcgacgt cggcgaccac ccggttgcgg atccgcaggg
     8761 cccgtgacag gcggttgtgt tcctcgggat cgagtagacc ctccgacagc gactcggcga
     8821 tcatctccga aagctcaagg gtcgaaaccg tgttctccag ttcgcttttg gcctcgacgc
     8881 cgaacgcgcg caggatcacg tgcgcgcacc agtcgtaaac cgcgatgacc gggcgggcgg
     8941 cgcgcaccca caacaggtac ggcggcacca acagcatggc cgacgattcg gggccggcga
     9001 tcgcgatgtt cttcggcacc atctcgccga gcagcacatg caagacaacg acgattgtga
     9061 tcgcgatgac gaacgacacg gtgtgcagga tggtgtcggg gatgccgagc aagtggaacg
     9121 gtttctccaa caggtgcgcc accgccggtt cacccacccg gcccaacagg atcgaacaga
     9181 tcgtgatgcc gagctgagcg cccgccagca tcagcggcag attctcgccg gcccggatca
     9241 ccgtcaccgc actgtgcttg ccttgctcgg caagcgcctc gagccggtcg cggcgcgcag
     9301 agatcagtgc gaactccgcg ccgacgaaga acgcgttggc cgccagcaga agcgcggtca
     9361 ggatcacgcc gaagaggtca cccatcgttg ccgtgttcct cttcgcgcct gcccagctgc
     9421 gtcagctcca actggtcgat gcgccgcccg tccatccgca ccacggtggc cagccagcgc
     9481 accgggtcct ccagcggtgc gtcgggatcg aacgccgcca gctcgacgga ttcgccttct
     9541 tcggggatgt ggccgagctc ctgcagcacc aggccgccga tggtttcgta ttcgccctcg
     9601 gaggcccgga atccggttgc gtcggccact tcgtcgatgc gaagcagacc cgagacgtgc
     9661 cagccctggc cggcctgcac gacatcggcc gtcggctcgt cgtgttcgtc gcggatgtcg
     9721 ccgacgatct cctcgatgac gtcctccacg gtcaccatgc ccgcagtgcc gctgtattcg
     9781 tcgaccacca gcgcggtctg caagccgttg gcccgcagct gcgccatcac atcgtcgccg
     9841 tccaaggtcg acggcaccct ggcgacgggc tgggccaggg tggccagcct ggtcttcgtg
     9901 tgttgctcgg gggggacctc gaagacctgt ttgacgtgaa cgattccgat ggtttcgtcg
     9961 aggtcaccgt cgacgatcgg gaatcgcgag taaccgctct cgatggccgc cgccaccaga
    10021 tcggcgatgg tgtcgtcaac ctcgagggtc acgatttccg ttcgcggtgt catcagttcc
    10081 tcggccgtcc gcgagccgaa ctgcagcgaa cggtccacca gcgtcgcggt gacgggatcg
    10141 agcgaccccc gccgagccga gttgcgcacc agtgacccga gctcctgcgg tgaccgggcc
    10201 gaacgcagct cctccgcggg ctcgatgccc agccggcgca gaatccagtt cgccgttccg
    10261 ttggtcaggt ggatcaacgg agtgaacagc atcgagaaca gcaactgcgg accggccacg
    10321 acacgcgcga ccggcagcgg cttggccact gcaaggtact gagcgacgag ttcgccgaac
    10381 accatcgaca ccgacgtggc gatcaacagc gccagcacca acgcgagggt gctggatacg
    10441 tcgtgagata gcgacatcgc cgcgaagagc ggctccagca gacgcgcgac cagtggctcg
    10501 gcgagatatc cggtgatcaa cgtggtgatc gagatgccca cctgggcacc tgagagctgg
    10561 aaggacagcg tgcgatgcgc ccgctgcacg agctggtctc ggcgcccgcc gccacgcgca
    10621 ttcgcgtcga ccgtgctgcg ttccagcgcg gtcagcgaga attcggcgga gacgaaaacc
    10681 gcggtgccgg cggtgagaac caggaaggcc aacaccgcga gcactgtgaa catggcgctc
    10741 accgcgagtc cgccaagtgg gcgccgggca ctgggcccgg cttagtaggg gacggctcgg
    10801 cgtcggtcgg ctcgtgggac tgctgctcgg cccggaagcc cacggcctcg acgggtgcct
    10861 gcggcactgg ctgcctttcg cttggtcggc aatgaatgcc ccatgttagc cgtcgtcgct
    10921 gctcgacggc ctcaccatcc ggtgggtagc gggtgacctt cggcaaagcc ggcggcggac
    10981 tgcacgccga gcacgacctt gtcgtggagt tcggcgatat tcgccgcgcc gacgtaggtg
    11041 cacgtgctgc gcacgccgga ggtgatgtgg tcgagcagat cttccacgcc gccgcgcgac
    11101 gggtccagcc ccatccgcga cgtcgagatg ccctcctcga acagcgcctt gcgtgcccgg
    11161 tcgaacgaac tgtcggcggc ggtgcgggcg gcaacggcgc gcttggaggc catgccgtag
    11221 ctctccttgt acggctggtc ctcgcggtcg cgcatcaggt cgcccggcga ttcgtaggtg
    11281 cccgcgaacc aggacccgat catcacgttg gccgcgcccg cggccagggc cagcgcgacg
    11341 tcgcggggat ggcgaacccc gccgtcggcc cacacatgac cgccgagttg tcgtgccgcc
    11401 gcggcacatt cgactaccgc ggagaattgc ggcctgccga cgccggtcat catgcgggtg
    11461 gtgcacatcg caccgggccc gacaccgacc ttgacgatcg acgcgcccgc ggtgatcagg
    11521 tcgcgcgtgc cctcagccga caccacgttg cccgcggcca gcggcacccc gaggtcaagc
    11581 gacgacaccg acttgatcgc gtcaagcatt ttgagttgat ggccgtgcgc ggtgtcgatc
    11641 accagcaggt cgacgccggc ctcggcgagt gaacgggcct tggcgccgac gtcgccgttg
    11701 atgccgaccg cagcggcgat gcgcaaccga cccttgtcgt cgacggcggg ggtgtagata
    11761 cctgcccgga tcgcgccggt gcgggtcaac acaccggcca gcgtgccgtc gggttcggtc
    11821 aaaaccgcca cgtccaccag cgcgtgttca agcaagtcga acaccttgcg ggggtcggtg
    11881 cccgcgggtg ccgtgatgaa atcggagatc gcgacgtcgc ggacccgggt gaaccggtcg
    11941 acgcccacgc agtcggcctc ggtgaccagc ccgatcgggc ggccctcgaa caccacgacg
    12001 gcggtgccgt gtgcgcgctt gtggatcagc gccatcgcgt ccgacaccga atcgtcgggt
    12061 gacagtacaa ccggggtgtc gacgaccaga tcacggctct tgacgaagtc gaccgtgttc
    12121 ttgaccgctg aaatgggcag gtcctgagga agcaccacaa tgccgccgcg gcgcgcgatc
    12181 gtctcggcca tccggcggcc cgccaccgcg gtcatgttcg ccaccaccac cgggatggtg
    12241 gtgcccgacc cgtcgtgcgt cgagaggtcg acgtcgaacc gcgactgcac gtccgagcgt
    12301 cccgggacga tgaagacgtc gttgtaggtc aggtcgtagg cggggtcgtg cccctcaaga
    12361 aatcgcatcg acctcgattt taggcccgat taggccggga cctcgctgtg gtcgccgctc
    12421 cacagcgtgt ggaacttctt atcgcggtcg tcgtcgatgc gcgcgtaggt gtgcgcgccg
    12481 aagaagtcgc gcagaccctg gatcaacgcc gcgggcagcc gctcggtgcg cagcgcgtcg
    12541 tagtacgaca gcgctgagga gaagccgggg atcgggatcc cgagttgcgt cgcggtgacc
    12601 accacccgcc gccagctgtc gatcgcgttc tccaccgcac tgcggaagta cggcgcggtg
    12661 atcagcgtcg gcagattggg gtcctcgtcg aaggcttcct tgatccgatt gaggaacttc
    12721 gcgcgaatga tgcagccgcc gcgccatatg gtggccagat caccgggggt gatgccccag
    12781 tcgtattcgg cgctgcccgc ctggatctgg ttgaacccct gcgcgtaggc aacgatcttc
    12841 gacgcgtaca gcgcctgccg cacgtcctcg acgaatttat ctgcgccgct tggcttttca
    12901 ccgagatcac ccgacgccag gccggtcgtt gcgtgccgct gcgcgaccga accagatagc
    12961 gcacgggcga acaccgcctc ggcgataccg gtaaccggca cgccaaggtc gagcgcggac
    13021 ttcaccgtcc agcggccggt gcctttctgc tcggcctcat cgaggatgac gtcgacgagc
    13081 ggtttgccgg tcctggcgtc gacctggcgc agcacctcgg cggtgatctc caccaggaag
    13141 ctgtccaggt cgcccttgtt ccattcggtg aacacgtcgg cgatctgcgg tgcggatagt
    13201 ccgagcccat cgcgcaacag ttggtaggcc tcaccgatca gctgcatgtc ggagtactcg
    13261 atgccgttgt gcaccatctt cacgaaatgc cctgcgccat cgggcccgat gtgcgtgcag
    13321 cagggcacgc cgtcgacgtg tgcggagatc tcctcgagca gtggaccgag cgacttgtag
    13381 gactcagccg ggccgccggg catgatcgac ggaccgttga gggcgccctc ctcgccgccg
    13441 gagatacccg cgccgacgaa gtgcagacca cgctcgcgca tcgccttctc gcggcggatg
    13501 gtgtcggtgt agagcgcgtt accgccatcg atgatgatgt cgccttcttc catcgcgtcg
    13561 gcgagctcgt tgatgacggc gtcggtggga tcgcccgcct tgaccatgat cagcactcgg
    13621 cgcggtttct caagggcggc aaggaattcc ggaatggtct cgctgcgcac gaacttgcct
    13681 tcgtcgccat ggtccttgag caatgcgtcg gtcttggcga tcgaccggtt gtgcagagcc
    13741 acggtgtaac cgtggtgcgc gaagttacgg gcaatgttcg agcccatcac ggccaagccg
    13801 gtgaccccga tctgagcggt gccgttggtg ggtgattcag acgagctcat tcgggcgcct
    13861 ttcattgttt gttgtgaagc ggtatcggct acctaatcac tacgccgtga acagccgatg
    13921 taattccgtc agccacggca ccgccagcgc cagggtaggc accacgagca ccgcggccgc
    13981 ggcgagatac gcggtggcgg caaaggcgcg gctgttcggc cggccgccga ggcggcgcac
    14041 ccgcagcacg gtcgtcggac cgccgacggc cagggcgccc gacggggtac gtccggctgc
    14101 gcaggcgacc agggcgcggg ccaggggagt ggggcctgcg gcgcgcaccg cggcgtcgtc
    14161 ggcgagcatc tcgatgagca gccgcacggc gtcaagagcg ttgccgctgc ggacgaatcg
    14221 ggggaaggcg gcgtgcaccg cggtgaacat ctccaggacc aggtcgtggc gggcgcgcag
    14281 gtgggcgcgt tcgtgggaga ggatcgcggc gatctcgttg tcggacaagg tggtcagcgt
    14341 tccttggcta accacaacgc ggctgcgcac cccgggcagg cagtaggcca gcggctcttt
    14401 gacatcgagg atgcgcagcc caccctgggg cttgccgaca agatcgacaa ccatgcggtg
    14461 gtgcgcccgg cggcgacgag tcgcgattgc gacctgcagc accgcgattg tgagtcgtgc
    14521 gccgatcacc agggtgagcg cgaagaccag caggtacgcg atccagaccg gccaacccag
    14581 tacgtcgatc tcactggtga ttgtggcggt ggggcggccg tcgggcccag gcacgaacag
    14641 ccggctggcg attgcgatgc cagccgagaa ggccgacaac acggcggcaa gggcgatgga
    14701 ctgccacagc acgatggccg cccgcggtgc gcgcatcggc caggtcgccc gcgccagcat
    14761 tgccgggacc ggtcccgaca ggaccagcgc gacgatggtg aaggccagcg cggacacgct
    14821 gtcagtattt ctcagccggt gcccggattg ccagcgggcg gggcttccgt gttcttgctt
    14881 tccaactcgg ccaacgcgcg gcgcagcgcc gcggcttcgt cggcgccgac acgttcgacg
    14941 aagtgcacca gcgcggcctg ccgactgccg gtgtcggaga cctggtcgag cgcgtcgacc
    15001 atcagcccgg caaccagctc atcgcggccg tgggtgggtg cgtaccggtg cgcccgatcg
    15061 tcgcggtgct gcacgacgag gttcttcttc gcgagccgct gcagcacggt catgatcgtg
    15121 gtgtaggcca gatcgcggcg ggccgacagc gcctcgtgga cctggcgcac ggtttggggc
    15181 tcgcgcgagg accacaggtg gtccatcaca gcgcgttcca ggtcccctaa acgcgtcagc
    15241 ttggccatat tccgttcatc tcctaccggc agtaaatcca gggtactacg gggttactac
    15301 gtcgcgtcgt aatccaacgc gccagaacaa cttcttgcag tgcgccgaat gttccggcga
    15361 cgtacaccgc ctggtagttg gcccttgtga gtccagtcac acggggtagc ccctcagcac
    15421 ggcatgacta tagtaaggct aacctaactc aagatcggga ggtgccatga cggttctgat
    15481 cgaggatccg ctcatcgcgg gcatggcgat caggcggacg ctgccactgc acgagtccag
    15541 ccgccggctg cgcgagctcc atcccgaatg tccccgcgtc tacggcgtcg ccgtgatggg
    15601 cgatctgtcg cgccggcggt ggtggccgct ggctgatgcc gtgacgactg atcggctgca
    15661 gacgatgttc gatatcggcg cggcggagac cgacagccgg gcctcgatag cccagcagct
    15721 ggccgccacg ctggcccacg tcgttgtcgg ccgcgtcgtg ccgctgctcg tgctcgaggg
    15781 gcgcgcctgg gacaccgggc tggagaacct ctgggtgcac gtcgactccg agggcacgat
    15841 cgactgggtc ggcgtcgtcg acccgcagct gcgggcgctg cccgacgacc ccgccctggc
    15901 cgacggagtc ccctcggctg gcatcgtcgc gctgcccagc gaggcggcgt tgacgacctg
    15961 ggtcgcccac cgcagtcacc gcgcgctgga tcccctgttc gccaggctcg tcgaggtctg
    16021 cgccggcgtc atgtcgatgg cgtcgatgtg gcacatcgtc ggcgcggcgg tggtcagcgc
    16081 ggcgacgcac gtgccgctgc tggctggttc gagcgaggtg acgagcatgc gtcgagcgca
    16141 ggcggtgctc gacgcgttgg tcgggttcgg cctgccggtc cgcggcgcca cgcggatagg
    16201 agcacggaag gccctgctta attaggcaag ccttgcctat tctagacacg accgaaagct
    16261 accggaccgc gccggagtcc tgagggctgc agagactccc ggtccctttc gaaggacggg
    16321 ccccacattc gcgtggggcc cgtcctcatt tccgcgttac ttgtcgatcg ctccgccgct
    16381 ttcggcccag tccttgaacg cgcccatgtt gtagacctcg gcgtaaccca gctccttgag
    16441 cgccttaccc gacagcgccg agcggccgcc ggacgcgcag tacaggatca cggtcttgtc
    16501 cttggcgaat tgcgcgtcgt ggtaggggga ttcgggatcc gcccggaatt cgagcatgcc
    16561 gcgggagacg tgcacggcgc ccgccacctt gccggactgc tccacctccg gcgcatcgcg
    16621 gacatcgacc acgagggcgt tgcccttggc gatcagttcc tgcgcctgtg ccggcgagat
    16681 cttgggcacc acatcgtttg cggcggctac gaggtctttc gcggttttgg ccatgcccga
    16741 cgatagctcc ggcccgattg cggaggagcg gtgtgcgccg tgtagacacg acggcgtgac
    16801 aacggaaggc gaattcggca gtggattcga caactggctc ggcctgacgt acatcgagat
    16861 gacacccgac ggtggacggg cgcggctgga gatcgacgac aaactgctgc agccgtgggg
    16921 catcgtgcac ggcggtgttt actgctcgat catcgagagc ctggccagtg tgtccgcgca
    16981 tgtctggttg tccgagaacg gcggtggcac cgtcgtcgga gtcaacaaca acaccgactt
    17041 cctgcgcgcc atctcggccg ggacggtcac cgcagtgtcg acgcccatcc accggggccg
    17101 tcggcagcag ctgtggttga tcacgatcac cgacgacaac gaccgcgtca ttgcccgcgg
    17161 ccaggtgcgg ttgcagaaca tcccgccgga gtagccccca attcgcgtgc gctgctgact
    17221 gccgcgcgcc gtggcaatat cgcgcactat gcgtttgacc ccgcatgaac aggaccggct
    17281 gctcatctcg tatgccgcgg agctagctcg tcgcaggcag gcgcgcggcc tgcggctgaa
    17341 ccaccccgag gctgtggcgc tgatcaccga ccatcttctc gagggcgcgc gcgatggtcg
    17401 caccgtggcg gagctgatgg tcagcggccg cgaggtgctg tcccgcgacg acgtcatgga
    17461 cggggtcccg gagatgctct acgacgtcca ggtcgaggcc acgtttcccg acggcaccaa
    17521 gctcgtcacc gtccatcagc cgatctcatg attccggggg agatcgtcta cggcgacggg
    17581 gacatcgaga tcaaccccgg cgcggcacgg ctcacgctcg atgtggtgaa tacgggcgat
    17641 aggccggtgc aggtcggcag ccacgtgcac ctgccacagg ccaacgcctc gctgcagttc
    17701 gaccgcgacg ccgcacatgg ccaccggctc gacatccccg cgggtaccgc tgtgcgattc
    17761 gaacccggag tcgcccagca tgtttcgttg gtgccgcttt ccggctcccg cgaggtccac
    17821 ggcctcagct tgaatccgcc cggccgattg gacgcctcat gaccgaattg tcgcgcgccc
    17881 gctacgccgc gctgttcggg ccgacgaccg gcgaccggat ccggctggcc gacaccgacc
    17941 tgtttgtcga gatcaccgag gaccgcagtg gtggacccgg attagccggc gacgaggcgg
    18001 tgttcggcgg cggcaaggtg ctgcgcgagt cgatggggca gggtcgagcc acccgcgccg
    18061 acggcgcacc cgacaccgtg attaccggcg tggtgatcat cgaccactgg ggaatcatca
    18121 aggccgacat cggcattcgt gacggcagga tcgcagcgat cggcaaggcg ggcaacccgg
    18181 acatcatgac cggcgtgcac cccgatctgg tggtcgggcc gtcgaccgag atcatctccg
    18241 gcaatggccg catcgtcacc gcgggcgcca tcgactgcca cgtgcatctc atctgcccgc
    18301 agatcatgga ggaggcgctc ggcggcggca tcaccacgat catcgccggc ggcaccggtc
    18361 cggccgaagg cagcaaggcc accaccgtca ccccgggatc ctggcacctg gcccgcatgt
    18421 tcgagtcgct cgactcctgg ccgctcaaca tcgccctgct cggcaagggc aacaccgtca
    18481 gcgccgaggc gatgtgggag caattgcgcg gcggcgcagc cggattcaag ctgcacgagg
    18541 actggggcac gacgcccgct gcgatcgacg cctgcctgac ggtgtcagac gcggcgggtg
    18601 tgcagtgcaa tattcacacc gacaccctca acgaagcagg cttcgtcgag gacacgctgg
    18661 ccgcagtcaa aggccgctcg atccacgcct accacaccga gggcgcgggc gggggtcacg
    18721 cacccgacat catcaccgtc gtatcgcacc cgaacgtgtt gccgagttcg accaatccga
    18781 cccggccgca taccctcaac accctcgacg aacacctcga catgctgatg gtctgccatc
    18841 acctgaaccc cagcgtccct gaggatctgg cgttcgccga gagccgcatc cggccatcga
    18901 ctatcgcggc cgaggacctg ctgcacgaca tcggtgcgat ctcgatgatc ggcagcgacg
    18961 cacaagcgat gggccgcatc ggcgaggtgg tgctgcgcac ctggcagacc gcgcacgtga
    19021 tgaagcgccg acgcggcgcc ctggagggtg acagtaaggc cgacaacaac cgggcccgcc
    19081 gatacgtcgc caagtacacg atctgcccgg ccatcgcaca cggtctcgac cacgagatcg
    19141 gctccgtcga ggtcggcaag ctcgccgacc tggtgctgtg ggaaccggcg ttcttcgggg
    19201 tccgcccgca cgcggtgctc aagggcggca tgatcgcttg ggcggcaatg ggagacgcca
    19261 acgcatcgat tccaacgcca cagccggttc tgccgcggcc aatgttcggc gccgcgcccg
    19321 cagcggccgc ggccacctcg gtgcacttcg tgtcgcagca ggccatcgag gacgggctgg
    19381 ccgaccggat cgacgtgaaa cggcgcctcg ctgcggtcaa ggatgtccgc aaggtcggca
    19441 aggccgatat gcccctcaac gacgcgctgc cgcacataga agtcgacccc gacacgttca
    19501 cggtccgcat cgacggcgat gtgtggcagg agcaacccgc cgccgaattg cccatggcac
    19561 aaagatattt cctgttctga tgaccaatct gtcgacactg ctggccctgg ctgattcgcg
    19621 gctgccgacc ggagggcacg tgcactccgg cggtgtcgag gaggcggtca ctggtgggct
    19681 ggtggccgac ctcaccacgc tgcgggccta tctgtggcgc cggatccgta cacacggcct
    19741 cgtcgcggca tcgctggcgg ccgccgtgca tgcaggctcc ctgacgatcg aagaggctga
    19801 ccgcgaaacc gatgcccgca caccggctcc cgctgcgcgg cagtcgtcgc gagcccaggg
    19861 tcggggtctc gcacggctgg cgcggcgagt gtgggccgac catgattggc agccgctcgg
    19921 aacgacgcca catctcgcgg tcgcggccgg tgcggtcggc aaggccagcg ggctcaaccc
    19981 ggaccagacc gcactgtcgg tcgtctacac cacaatgacc ggttccgcga cggccgcaca
    20041 gcggctgctg gcgctcgatc ccgccgacgt cgcggccctg accttcgagc tggcaccgct
    20101 gtgtgaggcg accgccgccg aggccaccaa ggagctggcc gacctgtccg acccactgct
    20161 cgacgtgctg gcccagcggc acagccaacg cgagcgacca ttgttcgtct cctgaaaggc
    20221 agaccatgcc accgcattac ctcgacggcg agccacacgc ccacaccgaa cggcccaggc
    20281 gccagcggca actgggggag ccactgcgca tcggcgtcgg cggacccgtc ggctccggca
    20341 agaccgcgct cgtcgcggcg ctatgcaggc agctgcgcga cgatctctcg ctggcggtgc
    20401 tgaccaacga catctacacc accgaggacg ccgacttcct gcgccgccat gccgtgctgg
    20461 ccgacgagcg gatcgccgcg gtgcagacgg gcggctgccc gcacaccgcg atccgcgacg
    20521 acatcaccgc caacctcgac gccatcgacg atctgatcgc acacaacgac cggctcgacc
    20581 tgattctggt ggaatccggc ggcgacaacc tgacggccac gttctcgtcg ggactcgtcg
    20641 acgtgcagat ctttgtcgtc gacgtggcag gcggcgacaa ggtgccgcgc aagggcgggc
    20701 ccggcgtcac cttttcagat ctgttggtgg tcaacaagac tgaccttgct ccgttggtcg
    20761 gtgcggatct cgaggtgatg cgacgcgatg cggccgacgt ccgtggggac cggccgacag
    20821 tgctgatctc attgaccgaa gaccccgccg cgacggcggt gctggcgtgg gtgcgagaac
    20881 agctgtgcgt tccgacgtcc tgatcgtcgc ccaccgcgac cgtcagccgc gcatcgagtg
    20941 cgggggagga gtcgcggcac ggcgcaccga acccgacacc gtgcatctgg tttcggcggc
    21001 cgccaccccg ctcggcggcg acgcgatcca catccgcgtg gtggtcgaac cgggtgcgca
    21061 attgcgggtg cgcaccgcgg cggcgaccgt gacgctgccc ggatcggcca cggtggaatc
    21121 gcatgcgtcc tggacgctcg aggtggcggg cgatctggac ctcgacccgc agccgaccgt
    21181 cgtcgctgcc acgtcgcgac atttcacctc gacccgcctg gagctgaccg gagccggccg
    21241 cgtccggctg agagaacgca tccagatcgg cagatccgat gagcggcaag ggttttggtc
    21301 gggatcgctg catgccgatc tcgacgggtc accgctgctg cggcacggca tcgagctcgg
    21361 taacgggtcg gtagccgacg acgcgctggg gacaccgatg gcgtgcgtca gcgaattcca
    21421 ctatcccgac agtgatgtcg agacccaggg cgttgcgctg gcgttggccg gcggcggctg
    21481 cctgtccacc tggcagggcg acaggctcta gctcgccgct ctttcgtcct gaacgcccgc
    21541 cgcgatctcc ttgagctctt cgatcctcgt gcgcgcgtac gcctgctgct cggtgatggt
    21601 gaggttccca cgcgctctcg acaggaacgt gaccgtccag gacagcaacg tcgtgacctt
    21661 ggtcttgaaa ccgacgagat acaccaagtg cagcaccagc caggcaagcc aggcgatgaa
    21721 gccaccgaat tcgatcgggc cgatcttggc gaccgcggag aaccgcgaca cggttgccat
    21781 cgagcccttg tcgaagtatt ggaacggctc ccgctgcgcg ggatcggcgc ccttcagctc
    21841 cgccttgatg gccttcgccg cgtacttggc gccctggatc gcgccctgcg ccattcccgg
    21901 cacaccttcc acggccgcca tgtcgccgac gacgaagacg ttccgatgcc ccggcaggga
    21961 caggtccggc agcaccttga cccgtcccgc gcggtccagc tccacgtcgg actggtcggc
    22021 gaggtcgcgt cccagcgggc tggccgacac accggccgac cacaccttgg tcgccgactc
    22081 gatgcgccgg atggtgccgt cggaatcctt gaccgtgatc ccgttgcggt ccacatcagt
    22141 gaccatcgcg cccaactgga tctcgacgcc catcttctcc aaccgggccc gggccttcag
    22201 gccgagtttc tcgcccatcg gcggcagcac tgcgggcgcc gcatcgagca ggatcacccg
    22261 cgcctgcgtc gaatcgatgt ggcggaacgc ccctttgagg gtgtggtcgg cgagttcggc
    22321 aatctgcccg gccatctcga caccggtggg cccggccccg acgacggtga acgtcagcag
    22381 cttctcgcgg cgcgcgggat cacttgagcg ctcggcctgt tcgaacgcgc cgaggatgcg
    22441 gccgcgcagc tcgagtgcgt cgtcgaccgt cttcataccg ggcgcccact cggcgaaatg
    22501 gtcgttgccg aagtaggact gccccgcacc cgccgcgatg atcaagctgt cgtaaggcgt
    22561 gacgtaggta tgaccgagca gctccgactt gaccgtcttg ttcgccaggt cgatgtgcgt
    22621 cacgttgccc aacaacacct gtgcgttctt ctgcttgcgc aggatgatcc gggtgggcgg
    22681 ggcaatctcg cccgacgaga tgatgccagt
//
LOCUS       DASRJA010000077        10552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3318240,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000077 DASRJA010000000
VERSION     DASRJA010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10552
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3318240"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VFC01_03580"
     CDS             complement(<1..646)
                     /locus_tag="VFC01_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HZN78759.1"
                     /translation="MTYDPGRRGSGRVPVLRDEWREPLRAQRDPLAEDSGRVRSNRDE
                     HRRVRKQTWLGRFISTYGWRAYALPALIALTAFVVYQTVTGTNAPAPKEVEGPVQGPP
                     TIGVTSTQIIGAPPKGLTQFDVNLPTGILPDGGPITETGAKTWHIVPGTTPKVGEGTT
                     KTFTYTVEIEDGIDTTTFGGDDGFARMVSETLANPKSWTHNPQFAFTRIDSGEPD"
     gene            776..1450
                     /locus_tag="VFC01_03585"
     CDS             776..1450
                     /locus_tag="VFC01_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78760.1"
                     /translation="MSDLANTAARRGAQPANGGAVSGAGRRGNRLPRDERRGQLLAAA
                     SEVFVDRGYHAAGMDEIADRAGVSKPVLYQHFTSKLELYLAVLQRHADNLVSGVRQAL
                     RTTTDNRQRVRAAVDAFFDFIEHDGQGYRLIFENDYVTEPQVYAQVKVATEACTDAVF
                     DLISHDSGLEPHRARMIAVGLVAISVDSARYWLNNDRPISKDDAVEGTVQFIWGGLSH
                     VPLTRS"
     gene            complement(1437..1694)
                     /locus_tag="VFC01_03590"
     CDS             complement(1437..1694)
                     /locus_tag="VFC01_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HZN78761.1"
                     /translation="MEVKIGVTDSPRELIFNSAQTPSEVEKLIADALGKDSGVLALTD
                     EKGRRFLVQSAKIAYVEIGAADIRRVGFGVGAVGSEAVKSG"
     gene            1978..2715
                     /locus_tag="VFC01_03595"
     CDS             1978..2715
                     /locus_tag="VFC01_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78762.1"
                     /translation="MNRQYTAHSPYSLSPTERIPSHGDDGETVDLDRERNDVYPGLEE
                     YDDYEDIEEYYEPIDRRWVWIAGVAGAILLVAVICTVVILGGGDSGSVSTTVAPPVIT
                     SQPAITSAPPAATTSSSPPPAATLPPETVTTVTPTASATPSEAPTESAPPPTPGPNTI
                     TYRVTGNRNLLDLVTVIYTDSQGALQTDVNVALPWAKTIVLDPGVTLSSVTATSVAGQ
                     LNCSIADASGTAIAVQNNNTMIANCTK"
     gene            complement(2717..3409)
                     /locus_tag="VFC01_03600"
     CDS             complement(2717..3409)
                     /locus_tag="VFC01_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HZN78763.1"
                     /translation="MTSTPPAASPEQTASGVSADHPGVNELFALLAYGEVAAFYRLTD
                     EARMAPNLRGRINMASMASAEMNHYEVLRDALERRGVDVVPAMTKYASALENYHRLTT
                     PSTWLEALVKTYVGDALAADFYLEIADAMPDEVADVVRAVLSETGHSQFVVAEVQAAV
                     TASEKQRHRLALWSRRLLGEAITQAQFVMAEHDELVDLVMSSGEGLTQMTEFFDRLQR
                     THSSRMHELGLA"
     gene            3653..5116
                     /locus_tag="VFC01_03605"
     CDS             3653..5116
                     /locus_tag="VFC01_03605"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN78764.1"
                     /translation="MTHLNPKFAELGVRDEIVRALAEDGKEHPFAIQELTLPLALAGD
                     DLIGQARTGMGKTLAFGVPLLQRITTDTERPLSGIPRALVVVPTRELCLQVSGDLAMA
                     AKYLTAGERKLTVTSIYGGRPYEPQIEALQKGVDVVVGTPGRLLDLAQQGHLRLGGLS
                     MLVLDEADEMLDLGFLPDIERILKQIPDERQAMLFSATMPDPIITLARTFMNQPTHIR
                     AEAPHSAAIHDTTEQFVYRAHALDKVELVSRILQANGRGATMIFTRTKRTAQKVSDEL
                     AERGFKVGAVHGDLGQAAREKSLKAFRTGAIDVLVATDVAARGIDIDDITHVINYQIP
                     EDEQSYVHRIGRTGRAGKTGIGVTLVDWDELTRWETIDKALGLACPDPAETYSSSPHL
                     YEELDIPTDAKGTVGEPRKAQAKRAPADKPADKPARNRTRNRRRTRGGKSTSGHPEAA
                     AEQTEPTGDDAPADGAEHSGRRRRRRRPRKAAAANAG"
     gene            5135..6346
                     /locus_tag="VFC01_03610"
     CDS             5135..6346
                     /locus_tag="VFC01_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78765.1"
                     /translation="MVKPERRTKGDMVAAAAIAAVIAVVAALIWWTSDARATISRPAA
                     TPAPTLPSAKAVPASLRQLWTAPSPKTTTPMVVGGNVVTGDGREVDGRDPATGNTRWT
                     YARDLELCGVTYVYNYAVAVYPDVRGCGQVSTIDAKTGERGPSRTAYADPEVRLSSDG
                     TTVLSAGDSRLEQWRSDMVRMLSYGALDARIKPGTAASPICRLVDAQASSTAVSVLEA
                     CPKQNDLRLTLLRPADEEDTPNVKFVPQPGVVDDSGARVVAVSDTTTAVYVPIPKPTL
                     NIVDETGATIASTLVPKPPSPNAVMSRAGDLITWWTGDSVMVFAANGLRYRYTVSAAG
                     NNMPLGPATVMAGRLLVPVTGGYDVFDPATGSGVAHLPVQRPPSSAAVVPAVAGSTII
                     EQRGDELVALG"
     gene            complement(6343..7146)
                     /locus_tag="VFC01_03615"
     CDS             complement(6343..7146)
                     /locus_tag="VFC01_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZN78766.1"
                     /translation="MNTVTRVLAVANQKGGVAKTTTVASLGAAMEEKGKRVLLVDLDP
                     QGCLTFSLGQDPDKLPVSIHEVLLGDVEPDAALVDTSEGMTLLPANIDLAGAEAMLLM
                     RAGREYALKRALAKVGGNFDVVIIDCPPSLGVLTLNGLTAADDVIVPLQCETLAHRGV
                     GQFLRTITDVQQITNSDLKLLGALPTLYDSRTTHSRDVLLDVADRYAMPVLAPPIPRT
                     VRFAEASASGASVLASRKNKGAHAYRELASALLKHWKNGKPLATYTPDL"
     gene            7197..7808
                     /locus_tag="VFC01_03620"
     CDS             7197..7808
                     /locus_tag="VFC01_03620"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HZN78767.1"
                     /translation="MGLLQHRLILLRHGETPWSKSGQHTGRTELDLTETGREQAKLAA
                     DALSVLTLDNPLVVSSPRRRALVTAELAGLKVDEVSPLLAEWDYGDYEGLTTQQIRQK
                     VPDWLVWTHGCPGGESVQQVSDRADRAIAYALKHMESRDVLFLGHGHFSRAVLTRWVE
                     LEISEGIRFAMVAASIAVCGFEHGVRQISALGLTGHRHSCLPA"
     gene            7805..8902
                     /locus_tag="VFC01_03625"
     CDS             7805..8902
                     /locus_tag="VFC01_03625"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HZN78768.1"
                     /translation="MTREPAFVLAGRTGVVVAEGVHTAFPRLADARAALASHSAPIIV
                     GALPFDISKPAALIRPQAVQFTETLPEWPLRELPTARITQMIPDPEEHRARIGAALRR
                     LQDAASGLHKVVLARALRLVAEGPLDARTIVHRLVANDGAANAYLVDLTAAGGGYSGA
                     VLVGASPELLVARRGDQVTCRPFAGSAPRMADPDADEASGAGLAASAKNRHEHQLVVD
                     AMRDALDPLCVELQIAPEPQLSKTAAVWHLYTPITGTLREKSTTALDLAVALHPTPAV
                     GGVPAMAAAELINELEGDRGFYAGAVGWCDQRGDGRWVVSIRCAQLSADRRTADAHSG
                     GGIVAESDPDDEVAETTTKFNTILSALGVHP"
     gene            9054..9536
                     /locus_tag="VFC01_03630"
     CDS             9054..9536
                     /locus_tag="VFC01_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN78769.1"
                     /translation="MSEVIRPAQPGDETELTAMIHELAEFERASADCTVTESQLREAL
                     FGEHPTVNGHIAEVDGRAAAGALWFRNFSTWDGVAGIYLEDLFVRPQFRRRGLARKVL
                     ATLARECVNSGYTRLSWAVLDWNVNAIALYDAIGGRPQSEWITYRVSGPELSALADES
                     "
     gene            complement(9488..9916)
                     /locus_tag="VFC01_03635"
     CDS             complement(9488..9916)
                     /locus_tag="VFC01_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78770.1"
                     /translation="MIVPSPSTVRQAAVVVTLEGAALVGVAVVLVVRHLAGVEDLPWL
                     SGYGTAAWFAIMGAGVLAAGWALWTGRRWGRGIAVFAQLLLLPVAWYMGVGSQRWLYG
                     VPVGLAALATLALLFSPSALQWLGAQDSSAKADNSGPETR"
     gene            9956..>10552
                     /locus_tag="VFC01_03640"
     CDS             9956..>10552
                     /locus_tag="VFC01_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZN78771.1"
                     /translation="MRAVLIVNPNATSTTPAGRDLLAHALESRVKLTVAHTDHRGHAI
                     EIAQDAARDGVDLLIVHGGDGTVNEVVNGILGEAGPDGTAPAVGVVPGGSANVFARAL
                     GISPDPIEATNQLVDLLSVYRRRKSWRRIGLMDTGERWCVFTAGMGVDGDVVAAVEAQ
                     RAKGRKVTASRYIRVAIREMLSSARKDPSLTLHIPGREP"
BASE COUNT         1822 a         3636 c         3502 g         1592 t
ORIGIN      
        1 aatcgggttc gccgctgtcg atccgggtga acgcaaactg cgggttgtgg gtccagctct
       61 tggggttggc cagggtttcg ctgaccatcc gggcgaagcc gtcgtcgccg ccgaatgtcg
      121 tggtgtcgat gccgtcctcg atctcgacgg tgtaggtgaa cgtcttggtg gtgccctcgc
      181 caaccttggg tgtggtgccg ggcacgatgt gccacgtctt ggcgccggtc tcggtgatcg
      241 ggccgccgtc gggcaggatg ccggtgggca ggttcacatc gaactgggtg agccccttgg
      301 gcggcgcacc gatgatctgc gtgctcgtca cgccgatggt cggcgggccc tgaaccggcc
      361 cctcgacctc cttgggcgcg ggcgcgttgg ttccggtgac ggtctggtac accacgaagg
      421 ccgtcagcgc gatcaacgcc ggcaacgcgt aggcccgcca cccgtaagtg gagatgaagc
      481 ggcccagcca cgtctgcttg cgaacgcggc gatgctcgtc gcggttggat ctgacccggc
      541 cggaatcctc ggcgaggggg tcgcgttgcg cgcgcaatgg ctctcgccac tcgtctcgca
      601 acacgggcac gcgaccgctc ccgcgacgcc ccgggtcgta ggtcaccgaa acaggatggc
      661 acagtctgct gcacttgcac ttccggcgca ccgccacctc gctatggcgt gcatccgcta
      721 tcgcagtggc aacggtagta atgtcggagc gaaacgcatt cagatgaagg acgtgatgag
      781 cgatctcgcc aacaccgccg cgaggcgggg cgcgcaaccg gcgaacggtg gcgcggtgtc
      841 cggcgccggc cggcgtggga accggctgcc ccgcgacgaa cgccgcgggc agttactcgc
      901 cgctgcgagc gaggttttcg tggaccgtgg ctaccatgcc gcgggaatgg acgagatcgc
      961 cgaccgcgca ggtgtaagca aacccgttct ctaccaacac tttacgtcca agctagagct
     1021 gtatctggcc gtgctgcagc ggcacgccga caatctcgtc tcgggtgtac gccaggcgct
     1081 gcgcaccacc accgacaacc ggcagcgggt gcgcgccgcg gtggacgcct tcttcgactt
     1141 catcgagcac gacggccagg gctaccggct gatcttcgag aacgactatg tgaccgagcc
     1201 ccaggtatac gcgcaggtca aggtggccac ggaggcgtgc accgatgcgg tattcgacct
     1261 gatcagccac gactccgggc tggagccgca ccgggccagg atgatcgcgg tcgggctggt
     1321 ggccatcagc gtcgactcag cccgctattg gctcaacaac gaccggccga tctccaagga
     1381 cgacgcggtg gagggcacgg tgcagttcat ctggggcggc ctgtcccacg tgccgcttac
     1441 ccgctcttaa cggcctccga gccgactgcg ccgacaccga agccgactcg gcggatgtcg
     1501 gcggcgccga tctcgacgta tgcgatcttc gccgactgca cgaggaaccg gcggcccttc
     1561 tcgtcggtca gagccagcac gcccgagtcc tttccgagcg catcggcgat caacttctcc
     1621 acctcgctgg gtgtctgcgc gctgttgaag atcagctcgc gcgggctgtc ggtgacaccg
     1681 atcttgacct ccacgctgga ccctttccgt tcgtgtgttc ctcgacttga aggctagtgg
     1741 acgcatgcgg cgcgatgccg ccccggccac ttcgcgggta gcgaaccgat gatccagcgc
     1801 gggtagcgaa ccgatgatcc agcgcggtta gcgaacccgc ggtacggcct tgccgcgcgg
     1861 attctcgacc gaaccgagtc cggaccgtga ccaaaggcgc agcgcgtcaa cctcattcgt
     1921 cacatcagcc tcgatagcat caggcccggc attgaaatat aaggaccggg atagcgcatg
     1981 aacaggcaat acaccgcaca ttcaccgtat tctttgagcc ccaccgaacg gatccccagc
     2041 cacggggacg acggcgagac cgtcgacctc gatcgcgagc gcaacgacgt gtacccgggc
     2101 ctcgaggagt acgacgacta cgaggacatc gaggagtact acgagccgat cgatcggcgg
     2161 tgggtctgga tcgccggcgt ggcaggcgcg attctgctcg tcgcggtgat ctgcacggtc
     2221 gtcatcctcg gcggcggcga cagcggctcg gtgtccacca cggttgctcc gccggtcatc
     2281 acctcacaac cagccatcac gtcggcacca cctgccgcga cgacctcgtc gtcgcccccg
     2341 ccggcggcga cgttaccgcc cgaaacggtc acgaccgtga cgccgaccgc cagcgccacg
     2401 cccagcgagg ctccgaccga atcggcaccg ccgcccacac ccgggccgaa cacgatcacc
     2461 taccgcgtca cgggcaatcg caatctcctc gacctggtga ccgtgatcta caccgactca
     2521 cagggagcgc tgcagaccga cgtcaacgtg gcgctgccat gggcgaagac gatcgtgctg
     2581 gatccgggtg tgacgctgag ctcggtcacc gcgacgagcg tggccggtca gctgaactgc
     2641 agcatcgccg acgcttcggg cactgcgatc gccgtgcaga acaacaacac gatgatcgcg
     2701 aactgcacca agtagctcag gccaggccga gctcatgcat gcggctgctg tgcgtgcgct
     2761 gcagccggtc gaagaactcg gtcatctggg tgaggccttc gccgctggac atgaccaggt
     2821 cgacgagctc gtcgtgctcg gccatgacga actgcgcttg ggtgatggcc tcaccgagca
     2881 gccggcgcga ccacagcgcc agccggtggc gctgtttctc gctggccgtc accgcagcct
     2941 gcacctcggc caccacgaac tgcgagtgcc cggtttccga cagcaccgct cgtacgacgt
     3001 cggccacctc gtcaggcatg gcgtcggcga tctccaggta gaagtccgcc gcaagcgcgt
     3061 cgccgacgta ggtcttgacc aacgcttcca gccacgtact cggcgtcgtc agccggtggt
     3121 agttctccag cgccgaagcg tatttcgtca tcgcgggcac cacgtcgact cccctgcgct
     3181 ccaaggcatc gcgcagcacc tcgtaatggt tcatctccgc ggaggccatg ctggccatgt
     3241 tgatccggcc acgcagattc ggcgccatgc gggcctcgtc ggtgagccgg tagaacgccg
     3301 ccacctcgcc ataggccaac agcgcgaaaa gctcgttgac gccgggatgg tcggccgaca
     3361 cgcccgaagc ggtctgctca ggagaggccg cgggcggcgt ggaagtcatg gcccaactct
     3421 agacgcggat gggcgcgccg accgccgggc aaaggcagcc agctatcatg gaagtaggca
     3481 gtggcccggc acgatcgaaa gtgaccagcc gctacctaag aaatgtgcgt gcacgcagtc
     3541 ggcccgcgac ctcaacgcag ggccctcggc cgtagcttcg tcgaactcgt gcgcgcgtgg
     3601 aaatccgcga ggacttacga ggttcgaccc agaaaggcta cgtcctaaca gcatgactca
     3661 cctcaatccg aagtttgccg aactcggagt tcgcgacgaa atagtgcgcg cgctcgccga
     3721 agacggcaag gaacatccct ttgctatcca agaactgacg ctcccactcg ccctcgcagg
     3781 cgacgacctc atcgggcagg cgcgcaccgg catgggcaag accctggcct tcggggtgcc
     3841 gctgctgcag cgcatcacca ccgacaccga gcgcccgctg tccggtatcc cccgcgcgct
     3901 cgtcgtggtg cccacccgcg aactctgtct gcaggtgtcc ggcgacctgg ctatggcggc
     3961 gaagtacctg accgccggcg agcgcaagct gaccgtcacg tccatctacg gcggcaggcc
     4021 ctacgagccg cagatcgaag cgctgcagaa gggtgtcgac gtcgtcgtcg gcaccccggg
     4081 gcggctgctc gacctggcgc agcagggcca cctgcggctg ggtgggctgt cgatgttggt
     4141 gctcgacgag gccgacgaaa tgctcgacct cggcttcctg cccgacatcg agcggatcct
     4201 caagcagatc cccgacgaac ggcaggccat gctgttctcg gcgacgatgc ccgacccgat
     4261 catcacgctg gcccgcacgt tcatgaacca gcccactcac atccgggctg aggccccgca
     4321 ttccgcggcc attcacgaca ccaccgagca gttcgtctac cgcgcccacg cgctggacaa
     4381 ggtcgagctg gtcagtcgca tactgcaggc caatggccgt ggcgccacga tgatcttcac
     4441 gcgcaccaag cgcaccgccc agaaggtctc cgacgagctc gccgaacgcg gcttcaaggt
     4501 cggcgcggtg cacggcgatc tgggacaggc cgcgcgcgag aagtcgctca aggcgttccg
     4561 caccggtgcc atcgacgtgc tcgtcgccac cgacgtcgcg gcccgcggca tcgacatcga
     4621 cgacatcacc cacgtcatca actaccagat ccccgaggac gagcagtcct atgtgcaccg
     4681 catcgggcgc acgggacgcg ccggcaagac cggcatcggc gtcacgctgg tcgattggga
     4741 cgagctgacc cgctgggaga cgatcgacaa ggcgctcggc ctggcttgcc cggatccggc
     4801 cgagacctac tccagctcac cgcatctcta cgaagagctg gacatcccga ccgacgccaa
     4861 gggcacggtc ggcgaaccgc gcaaggcaca ggccaagcgc gcgccggcgg acaagcccgc
     4921 cgacaagccc gcccgcaacc ggacccgcaa ccgcaggcgc acccgtggtg gcaagtcgac
     4981 cagcgggcac cccgaggcag cggccgagca gaccgaaccc accggggacg acgcgcccgc
     5041 cgacggcgcc gagcactccg gccggcgccg ccgtcgccgt cgcccgcgta aggccgcggc
     5101 cgcgaacgcc ggctagtcga gctccgtcgg acggatggtc aaacccgaac gccgcaccaa
     5161 aggtgacatg gtggcggccg cggcgatcgc cgcggtgatc gccgtcgtcg ccgccctgat
     5221 ctggtggacg agcgatgccc gggccaccat cagcaggccc gctgcaacgc cggcgccgac
     5281 gctgccgtcc gccaaggcgg tgccggcctc gctgcggcag ctgtggacgg cgccgagccc
     5341 gaagaccacc accccgatgg tggtgggagg caacgtcgtc accggcgacg gccgcgaagt
     5401 cgacggccgc gaccccgcca ccggaaacac gcggtggact tatgcgcgcg acctcgagct
     5461 gtgcggcgtc acgtatgtgt acaactacgc ggtcgcggtg tatcccgacg tccgcggctg
     5521 cgggcaggtc agcacgatcg acgccaagac cggcgagcgc ggccccagcc gcaccgcgta
     5581 cgccgacccc gaggtgaggc tgtcgtccga tggcaccacc gtgttgtcgg ccggggacag
     5641 ccgcctggag cagtggcgct cggacatggt gcggatgctg tcctacggtg cgctcgacgc
     5701 gcggatcaag ccgggcacgg ccgcctcgcc gatctgccga ctggtagatg cgcaggcgag
     5761 ttccacggcg gtgtcggtgc tggaggcgtg tccgaagcaa aacgacctac ggctcacgct
     5821 gctgcggccc gccgacgaag aagacacgcc caacgtgaaa ttcgtgccgc agcctggcgt
     5881 cgtcgacgac tccggcgcgc gcgtcgtcgc agtctccgac accacgacgg ccgtctacgt
     5941 gccgatcccg aagccgacgc tcaacatcgt cgacgagacc ggtgcgacaa tcgcgagcac
     6001 gctcgtcccc aagccgccgt ctccgaatgc ggtgatgtcg cgggccggtg atctgatcac
     6061 gtggtggacc ggtgattccg taatggtgtt cgccgccaac ggacttcgct accgctacac
     6121 ggtcagcgcg gcgggcaaca acatgccgct ggggcccgcg acggtgatgg ccgggcgatt
     6181 gctggttccc gtcaccggcg ggtacgacgt gttcgatccg gccaccggca gcggtgtcgc
     6241 gcaccttccg gtgcaacgcc cgccgagttc cgctgcggtg gttccggcgg tggcggggtc
     6301 gacgatcatc gaacagcgcg gcgacgaact cgtcgcgttg ggttagaggt ccggcgtgta
     6361 cgtcgccagc ggcttaccgt tcttccaatg cttgagcagc gcgctggcca actcgcggta
     6421 ggcgtgcgca cccttgttct tccggctcgc gagcaccgac gcgcccgacg cactggcctc
     6481 ggcgaaccgc acggttcgcg ggatcggtgg cgccagcacc ggcatggcgt agcggtcggc
     6541 gacgtcgagc agcacatcgc ggctgtgggt ggtgcgcgag tcgtacagcg tcggcaatgc
     6601 gcccagcagc ttcaggtccg aattggtgat ctgctgcacg tcggtgatag tgcgaagaaa
     6661 ctgaccgacg ccgcgatgcg ccaaagtctc gcactgcagc ggcacgatca catcgtcggc
     6721 ggcggtcagc ccgttgagcg tgagcacgcc cagcgagggc gggcaatcga taatcaccac
     6781 gtcgaagttg ccgccgacct tcgcgagcgc acgcttgagc gcatattcgc ggccggcgcg
     6841 catcaacagc atcgcctctg cgcccgccag gtcgatgttg gccggcagca gcgtcatccc
     6901 ctccgacgtg tcgaccagcg ccgcgtcggg ttccacgtca ccgagcagca cctcgtggat
     6961 cgacaccggc agcttgtcgg gatcctggcc cagcgaaaac gtcaaacatc cctggggatc
     7021 caggtcgacg agcagtaccc gtttaccctt ttcctccatc gccgcaccca acgacgcgac
     7081 cgtcgtcgtt ttggcaaccc cacccttttg attggcgacc gcaagtaccc gcgtcacggt
     7141 gttcatcgtt gcacgggtac ccatttatca acggacgtgc ggcagaatcg gtctccgtgg
     7201 gtcttttgca gcatcggctg attttgcttc gccacggcga gacgccgtgg tcgaagtccg
     7261 gtcagcacac cggccggacc gaactcgacc tgaccgaaac gggccgagag caggccaagc
     7321 tggcggccga cgcgctgtcg gtgctcaccc tcgacaaccc gttggtggtc tccagcccgc
     7381 gacggcgtgc gctggtcacg gccgaactgg ccggcctgaa ggtcgacgaa gtgtctcccc
     7441 ttctggccga atgggattac ggagactacg aaggcctgac cacgcagcag atccggcaga
     7501 aggtgcccga ctggctggtg tggacgcacg gctgtcccgg cggggaaagc gtgcagcagg
     7561 tcagcgaccg cgccgaccgg gcgatcgcgt acgcgctcaa gcacatggag tcgcgcgacg
     7621 tgcttttcct cgggcacggg cacttctccc gcgcggtgct cacgcggtgg gtcgagctgg
     7681 agatatccga ggggatcagg ttcgcgatgg tggccgcatc gatcgcggta tgcggcttcg
     7741 aacatggcgt gcgccagatc agcgcgctcg gactcacggg tcaccgccac tcgtgcctgc
     7801 ccgcgtgaca cgcgaaccgg ccttcgtgct cgccggacga accggcgtgg tggtcgccga
     7861 aggtgtgcac accgctttcc cccgactggc ggacgcacgt gccgcactgg catcgcatag
     7921 cgccccaatc attgtgggcg ccttgccttt tgacatatcc aagccggcgg cgctgatccg
     7981 accgcaggcc gtgcagttca ccgagacgct gccggaatgg ccccttcgcg agctgcccac
     8041 ggcgcggatc acgcagatga ttcccgatcc cgaggaacat cgcgcccgca tcggcgcggc
     8101 gttgcgcaga ctgcaggacg ctgccagcgg gctgcacaag gtggtgttgg cccgcgcctt
     8161 gcggttggtt gccgagggtc ctctggatgc ccgcaccatc gtgcatcggc tcgtcgccaa
     8221 cgacggtgcg gccaacgcgt atctggttga cctcacggcc gcgggcggcg gctattcggg
     8281 cgccgtgttg gtcggcgcga gcccggagct gctcgtcgcg cggcgcggcg accaggtgac
     8341 ctgccgcccg ttcgcgggat cggcgccgcg gatggcagat cccgacgccg acgaggccag
     8401 cggcgccggg ctcgccgcgt cggccaagaa ccgccacgaa caccaactgg tggtcgacgc
     8461 gatgcgcgac gcgcttgacc cgctgtgtgt cgagcttcag atcgcgccgg aaccgcaact
     8521 gagcaagacc gcggcggtct ggcacctcta tacccccatc accggcacgc ttcgtgaaaa
     8581 gtccactacc gcattggatt tggccgtcgc cctgcacccg acacccgcag tgggcggcgt
     8641 tccagcaatg gccgcggccg agctgatcaa cgaactggag ggcgatcgcg gcttctacgc
     8701 gggcgcggtc ggctggtgtg atcagcgcgg cgacggccgg tgggtggtgt cgatccgctg
     8761 cgcgcaactg tcggcagatc gccgcaccgc cgacgcacac tcgggcggcg gcatcgtcgc
     8821 cgaatccgat cctgacgacg aagtcgcgga aaccacaacg aaattcaaca cgatcctgtc
     8881 ggcccttgga gtgcacccgt gagcgcccga agacgaccgc gagtgaggat cgcagcgagg
     8941 cacgagtccc cgaagacgaa cgcgagtgag gatcgcagcg aggcacgagc gaggatcgga
     9001 gcgagcggga gtcgaggcag taacggagga ccggagcgag cgggagtcga ggcatgagtg
     9061 aagtcatccg cccggcccaa ccgggcgatg agaccgagct gaccgcgatg atccacgaac
     9121 tggcggagtt cgagcgggcc agcgcggact gtaccgtcac cgaaagtcag ttgcgtgaag
     9181 cgcttttcgg tgagcacccc acggtcaacg ggcacatcgc ggaggtcgac ggccgggccg
     9241 cggcgggcgc gctgtggttc cgcaactttt ccacctggga cggagtcgcc ggcatctatc
     9301 tggaggatct ctttgtgcgc ccgcagttcc gccgccgcgg cctggcgcgc aaggtgctcg
     9361 ccaccttggc acgcgaatgc gtcaacagcg gatacacccg gctgtcgtgg gcggtgctgg
     9421 attggaacgt caacgcgatc gccctctacg acgccatcgg cggacggccg cagtccgagt
     9481 ggatcaccta ccgggtttct ggcccggagt tgtctgcttt ggccgacgaa tcctgagcgc
     9541 ctagccactg cagcgccgac gggctgaaca gcagcgccag agtcgcgagc gcggcgagcc
     9601 ccacggggac gccgtacagc cagcgctgtg agccgacgcc catgtaccac gcgacgggaa
     9661 gcagcagcag ctgagcgaaa accgcgattc cacggcccca gcggcgtccg gtccacaacg
     9721 cccaccccgc agcgagcact ccggcgccca tgatcgcgaa ccacgccgcg gtgccatacc
     9781 cgctgagcca cggcagatcc tcgacgcccg ccaggtgacg cacgaccaac accacggcga
     9841 cacccaccag cgcggcgccc tcaagagtga cgaccacggc cgcctggcga acggtggatg
     9901 gcgagggcac gatcacgggg tcgagcctag aggttgccgg ataggctcgg actccgtgcg
     9961 cgccgtgctg atcgtgaacc cgaacgctac gtcgacgact ccggcgggcc gcgacctgct
    10021 ggcacacgcg ctggagagcc gcgtgaagct gaccgtcgcg cacaccgacc accgcggcca
    10081 cgccatcgaa atcgcccagg acgcggcccg tgacggcgtc gacttgctga tcgtgcacgg
    10141 cggtgacggc acggtgaacg aagtcgtcaa cgggatactc ggtgaggccg gacccgatgg
    10201 gacggccccg gccgtcggcg tggtgccggg cggttcggcc aacgtgttcg cccgtgcgct
    10261 gggcatcagc cccgacccga tcgaggcgac gaatcagctc gtcgacctgc tgtcggtcta
    10321 ccggcggcgc aagtcgtggc ggcgcatcgg gctgatggac accggggaac gctggtgcgt
    10381 gttcaccgcg ggcatgggcg tggacggcga cgtcgtcgcc gccgtcgagg cccagcgcgc
    10441 caagggccgc aaggtgacgg cctcacgcta catccgggtg gctatccgcg aaatgttgtc
    10501 cagcgcccgt aaggatccgt cgttgacgtt gcacatcccc ggccgcgaac cg
//
LOCUS       DASRJA010000078         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1626234,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000078 DASRJA010000000
VERSION     DASRJA010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626234"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2786)
                     /locus_tag="VFC01_03645"
     CDS             complement(<1..2786)
                     /locus_tag="VFC01_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78772.1"
                     /translation="MRTLSLVASLGLAVSAFSQDPGQELPSPKAAMADFLRAHGDSWI
                     TQWHPATGTPSAIFGPGLALENWNDNTLEHARQHALKLLQEHSDLLGLGTSTFREVCA
                     GRMGRTWSFVFDQFFRGLPVIEGRADVRINMNGKVAMFGSRAWPIPATFNITPVIAEQ
                     VAVANAWLALGETPGEVTQPGTPRAPQLVIWGDINAPASAPFYLAWEVPISNIDRNGK
                     GPIGRCYIDALTGGVLHYQNDRHECGMPGCTLATHSRKSAPRAIDTVPATGAPPVPTV
                     VTLMAWTRTGADGYSALVNTPLTDIVLNVPGIGTRTTDANGEFTIDIVSPVAITVGAL
                     DGTHHAPITGSLPPSAIVTVNPGVNTTIQLLTAASTGNEGAHPTAAYWVDKTNEYARS
                     IVGNTSQMSTASTIAVAVNVTGTCNAFYSGNSINFYSEGGGCPNLAFSTVISHEWGHG
                     LDDRYGGISNATGDGLSEGWGDIIGMYIVDSVLLGSGFQTPGVALRRGDNTRLYPQTG
                     AEVHTAGQVWMGFAWRYREALRTAFGTPQALQISEDTVIGSIIADATNQVDAVREVFV
                     ADDDDANLLNGTPHYAQLSAAAIAKNLPYPMIQVIAFSHAPLGNTTNRVTPRVVNCTA
                     ALVSGGAITAIRLHYNAGAGAVVRNMHPNGLVNGYRAMLPGQLSGSVSYHIEAVHNGT
                     TTVRLPTSGEYSYTTSVQTSGPFVGFHLQDFESGAAGWTHGLYSGITDDWQLGVPQGK
                     FSTVSGVAWADPTAAGSTGSVYGTDLGAGTSNGRYPNSVSYWLRSPAINCTGQFGCFL
                     RFKRWLTVEQGIFDQASVYVNGIQIWTNATATHHLDAAWQVVEYPIPMADNNAAVTVE
                     FRLTADVGLNLGGWNIDNFELGTKTIVPLEAELRLLPEQVVQGGTLTLAVQTGTNSRP
                     FLIGLGDLPGPIVVP"
BASE COUNT          472 a         1000 c         1129 g          555 t
ORIGIN      
        1 gggaccacga tcgggcccgg taggtcaccg aggccgatca ggaacgggcg cgagttggtg
       61 ccggtctgca cggccagggt cagcgttccg ccttgcacca cctgttcggg caggagcctc
      121 agctctgctt cgagcggcac gatggtcttc gtgccgagct cgaagttgtc gatgttccaa
      181 ccgccgaggt tcaacccgac gtcggcagtg agccggaact cgaccgtgac ggccgcattg
      241 ttgtcggcca tcgggatcgg gtactcgacc acctgccacg ccgcgtcgag gtggtgcgtg
      301 gccgtcgcgt tggtccagat ctggatgccg ttgacgtaga ccgaggcctg atcgaagatg
      361 ccctgctcga cggtcagcca gcgcttgaag cgcaggaagc agccgaactg gcccgtgcag
      421 ttgatcgcgg gcgagcgcag ccagtagctg acgctgttcg ggtagcggcc gttgctcgtg
      481 ccggcgccga ggtcggtgcc gtagaccgaa ccggtgctgc ccgccgccgt cgggtcggcc
      541 cacgccactc cggacaccgt gctgaacttg ccttgaggca cgccgagctg ccagtcgtcg
      601 gtgatgccgc tgtacaggcc gtgcgtccag cccgcggcgc cgctttcgaa gtcttgcagg
      661 tggaagccca cgaacggacc cgatgtctgg acactcgtgg tgtaggagta ctcgccactc
      721 gtgggcagcc gcaccgtcgt ggtgccgttg tgcacggctt cgatgtggta gctcaccgag
      781 ccgctcagct gtccaggcag catggcgcga tacccgttca ccaagccatt cgggtgcatg
      841 ttgcggacga cggcgccggc accggcgttg tagtgcaggc gaatcgccgt gatggcaccg
      901 cccgacacga gcgcggcggt gcagttgacg acgcgcggcg tgacgcggtt ggtcgtgttg
      961 ccgagcgggg cgtgcgagaa cgcgatgacc tggatcatcg gatagggcag gttcttcgcg
     1021 atcgcggccg cgctcagctg tgcgtagtgc ggcgtcccgt tgagcaggtt cgcgtcgtcg
     1081 tcgtcggcga cgaacacctc gcgcacggcg tcgacctggt tcgtcgcgtc cgcgatgatc
     1141 gacccgatga ccgtgtcttc cgagatctgc agcgcctgcg gcgtgccgaa cgcggtgcgc
     1201 agcgcctcgc ggtaacgcca ggcaaaaccc atccagacct gacccgccgt gtgcacttcg
     1261 gcgcccgtct gcggatacag gcgcgtgttg tcgccgcggc gcagcgcgac gcccggcgtc
     1321 tggaagccac tgccgagcag cacgctgtcg acgatgtaca taccgatgat gtcaccccag
     1381 ccttcgctga gtccgtcgcc agtggcgttg ctgatgccgc cgtagcggtc gtcgagaccg
     1441 tggccccatt cgtgcgagat gacggtcgag aacgcgaggt tcgggcagcc accgccctcg
     1501 gagtagaagt tgatcgaatt acccgagtag aacgcgttgc acgtacccgt cacgttcacc
     1561 gccaccgcga tggtgcttgc ggttgacatc tgggacgtat tgccgacgat cgagcgcgcg
     1621 tactcgttgg tcttgtcgac ccagtacgca gccgtcgggt gggcgccttc gttccccgtc
     1681 gatgccgccg tcagcagctg gatcgtcgtg ttgaccccgg ggttcaccgt gacgatggcg
     1741 ctcggcggaa gcgagccggt gatcggcgcg tggtgagtgc cgtcgagtgc acccaccgtg
     1801 atggcgacgg gcgacacgat gtcgatcgtg aactcgccgt tcgcgtcggt ggtgcgcgtg
     1861 ccgatgccgg gcacgttgag cacgatgtcg gtcagcggag tgttcacgag cgcgctgtag
     1921 ccgtcggcgc ccgttcgggt ccaggccatc agggtcacga cggtcggcac ggggggtgcg
     1981 cccgtcgcgg ggaccgtgtc gatggcgcgc ggggcgctct tgcgactgtg cgtggccagc
     2041 gtgcagccag gcatgccgca ttcgtgcctg tcgttctggt agtgcagcac gccaccggtc
     2101 agggcgtcga tgtagcagcg gccgatgggc cccttgccgt tccggtcgat gttgctgatc
     2161 gggacttccc aggcgaggta gaacggcgcc gacgcaggtg cgttgatgtc gccccagatg
     2221 accagctgcg gtgctctcgg cgtcccgggc tgcgtcacct cgccgggtgt ctcgccgagt
     2281 gcgagccacg cattggcgac ggcaacctgt tcggcgatga cgggcgtgat gttgaaggtc
     2341 gcggggatcg gccacgcgcg gctgccgaac atcgcgacct tgccgttcat gttgatgcgg
     2401 acgtcagctc ggccctcgat gaccggcaga ccgcggaaga attgatcgaa gacgaacgac
     2461 caggtgcgac ccatgcggcc ggcgcagacc tcgcggaacg tcgaggtgcc gagcccgagc
     2521 aggtcgctgt gttcctgcag cagtttcagc gcgtgctgcc gcgcatgttc gagcgtgttg
     2581 tcattccagt tctccagtgc gagcccaggg ccgaagatcg cgctcggcgt gcccgtcgcg
     2641 gggtgccatt gcgtgatcca agaatctcca tgcgcgcgga ggaaatccgc catggcggcc
     2701 ttcggcgacg ggagctcttg accgggatcc tgggagaatg cggagaccgc gagcccgaga
     2761 ctcgcgacga gcgacagtgt tcgcatgagg acttgacctg gcgggacttg tgctgcggcg
     2821 accgtcgggg gacaggcgct gcggagggac aacgaacgtc tcgaggtgaa ctcgaggcgg
     2881 gcaggatggg gcgagcgccg ggcagtagac gcgcacagcc acgggtggtc aacccagcac
     2941 ttacctcact tgcggaaatg gcactgcgtt gggcctgcac gctgcgttcc cgcgtgctgt
     3001 gacccggtag cccgtaagcg ccggcgcacg ggagtgcgcg tccgccgcgg gtggcgcgtc
     3061 tcctggtcgg cgatcgagcc gtcatctccg caagccgacg cgggttgggt cgatcggcca
     3121 ccgagcggcg gcttttttct gcaccccccg gcgccg
//
LOCUS       DASRJA010000079         3061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2780892,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000079 DASRJA010000000
VERSION     DASRJA010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2780892"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VFC01_03650"
     CDS             complement(<1..220)
                     /locus_tag="VFC01_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78773.1"
                     /translation="MRDADLRANSTRYLEVSNARFPQIYASTPTAMLRGMAFYWARIW
                     IEVTPVRVLWWPNGDLDAAPQIWMRDAAR"
     gene            complement(349..1605)
                     /locus_tag="VFC01_03655"
     CDS             complement(349..1605)
                     /locus_tag="VFC01_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN78774.1"
                     /translation="MTETAPAPTETTESVEEFAARARAWLADNMPPIDPNNPPEHDRG
                     EEEPWLRARELQKKLYEGGFAGICFPKEYGGLGLPIAYQKAFDNESRCYELPIILNTP
                     TFTICAATILDTGSEEQKQQHISAALRGDEVLVQLLSEPSGGSDLAGVITRADRVGDK
                     WVINGAKTWSTSAFAADYGLMLARTNWDVPKHEGLTMFLVPINSPGITLRRIKQVNGS
                     VEFCEEFFDNLELGDDAVVGEVGGGWQVASRQLYHERRAVGGGSEFASGIGAEGKSDV
                     PIDYVALLKKTGQADSEHARVLAGRALVHRAVREQLIDHVYHGVLDGSLPPAAGSIIR
                     VTHAETHHLEFDTALAISGSSGVVDGGDDVLRFGERYLSRQTAALGGGTTEIAKNIIG
                     ERILGFPREYAADRGVPFNQVKRNKS"
     gene            complement(1602..2738)
                     /locus_tag="VFC01_03660"
     CDS             complement(1602..2738)
                     /locus_tag="VFC01_03660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN78775.1"
                     /translation="MTEDANPERLLFASTTQSFLEKEASLARLRELHAAGASFEAGWW
                     QRAAELGWTSLLVPEDLGGGSVSGDGVADLALVAELAGRTVAPGPLHPVSTVLAGLVE
                     APENHQDTIESLISGEAVASWAVYEPGKPWAPLNTSVTATRTETGYRIDGVKDRVEAG
                     VESAVLLVVAQHEGAVRQFLVPTDAPRVRVEPQRSIDMVKSYARVHFDGVEVDDSAAV
                     GTAEQTAGLIARQSQIAQMLQCAEVVGILDTVLAFTIQWGFDRHSFGRPIGSYQALKH
                     KYADLKIWFEACRATTNAAVAEVASGSAGADMAVSVAKSYVGEHAPGMLQDCIQLHGG
                     IGVTWEHDLHLFLRRVTLYRSMFGTPEDHNLHVYALTEHLEPAS"
     gene            complement(2744..>3061)
                     /locus_tag="VFC01_03665"
     CDS             complement(2744..>3061)
                     /locus_tag="VFC01_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN78776.1"
                     /translation="NTYKKMPQAFPENPIEAFKRGVYVAPFWEDDFAQMAGLCGVDRV
                     IFGSDWPHPEGLAEPTHLIDDLQGLDEEGQRKVMGGNMIDLFKVENKIVHNPDVPPLV
                     IPA"
BASE COUNT          500 a         1028 c          955 g          578 t
ORIGIN      
        1 gtcgtgccgc gtcgcgcatc cagatctgcg gcgcagcgtc gagatcaccg ttgggccacc
       61 acaacacgcg caccggcgtg acctcgatcc agatacgtgc ccaatagaag gccatgccgc
      121 gcagcatcgc cgtcggagtg ctcgcgtaga tctgcgggaa gcgcgcgttc gaaacctcca
      181 ggtaccgagt cgagttcgcc cgtaggtcag cgtcgcgcac ggtcgccaac ccctgcacgg
      241 gttcgacgtg atggcggtga cgggctcgcc ctacgtgtac acgtatttca ccgtcgacgc
      301 cggctggtca cccaagcagt acgagcgatg gattgccgac gcgctgcctc agctcttgtt
      361 gcgcttgacc tgattgaacg gcaccccgcg gtcggcggcg tattcgcgcg ggaagccaag
      421 tatccgttcg ccgatgatgt tcttcgcgat ctctgtggtg ccgccgccga gcgccgcggt
      481 ctgacgcgac aggtatcgct cgccgaagcg gagcacgtcg tcgccgccgt cgaccacgcc
      541 cgagctgccg ctgatcgcca gcgccgtgtc gaactccagg tgatgtgtct ccgcgtgcgt
      601 gacccggatg atcgagcctg cggccggggg cagagagccg tcgagaacac cgtgatagac
      661 gtggtcgatg agctgctctc gcaccgcacg gtgcaccagc gcccggcccg ccagcacgcg
      721 ggcatgttca ctgtcggctt gacctgtctt cttcagcaac gcgacgtagt cgattggcac
      781 gtcggacttg ccttctgcgc caataccact ggcgaactcc gagccgccgc cgacggctcg
      841 ccgctcgtgg tagagctgcc gggaggccac ctgccagccg ccgccgactt cgccgacgac
      901 ggcgtcgtca ccgagttcga ggttgtcgaa gaactcctcg cagaattcga ccgatccgtt
      961 cacctgcttg atccgccgca gcgtgatccc ggggctattt atgggcacca ggaacatcgt
     1021 cagcccctcg tgcttgggca catcccaatt ggtgcgtgcc agcatcagcc cgtagtcggc
     1081 cgcaaatgcg ctcgtgctcc atgtcttcgc gccgttgatc acccacttgt cgccgacgcg
     1141 gtcggcccgg gtgatgacgc ctgcgaggtc tgacccgccg ctgggctccg acagcagctg
     1201 caccagcacc tcgtcaccgc gcagtgcggc cgaaatgtgt tgctgcttct gctcttcgga
     1261 accggtgtca agaatcgtcg cggcgcagat cgtaaatgtc ggcgtgttga ggatgatcgg
     1321 tagctcgtag caacgggatt cgttgtcgaa tgccttctgg tacgcgatcg ggagacccag
     1381 cccgccgtac tccttcggga agcagatgcc cgcgaagccg ccctcgtaaa gcttcttctg
     1441 aagctcacgt gcccgcagcc acggttcctc ttcacctcgg tcgtgttccg gcgggttatt
     1501 cggatcgatc ggtggcatgt tgtccgccaa ccatgcccgc gcacgggcgg cgaattcctc
     1561 gaccgattcg gtggtttcgg ttggtgccgg tgctgtctcg gtcatgaagc gggctccagg
     1621 tgctcggtga gcgcgtagac gtgcaggttg tggtcttcag gtgtgccgaa catcgagcgg
     1681 tacagcgtga ctcgccgcag gaacagatgc aggtcgtgct cccaggtcac cccgataccg
     1741 ccgtgcaact ggatgcagtc ctgcagcatg cccggagcgt gttcaccgac ataggatttc
     1801 gcaacgctga cggccatatc ggcgccagca gaacccgacg ccacctctgc caccgcggcg
     1861 ttggtcgtcg cgcggcacgc ctcgaaccag atcttcaggt cggcgtactt gtgcttgagt
     1921 gcctgatacg aacctatggg tctgccgaag gagtgccggt cgaaacccca ctggatggtg
     1981 aaggccagca ctgtgtccag aatgccgacc acctcggcgc actgcagcat ctgcgcgatc
     2041 tggctctgcc gtgcgatgag gcccgcggtc tgttcggccg ttccgactgc cgcggagtcg
     2101 tcgacttcga cgccgtcgaa gtgcacgcgg gcatagctct tcaccatgtc gatcgaacgt
     2161 tgcggctcaa cgcgtacccg tggcgcatcc gttggcacca ggaattggcg caccgcaccc
     2221 tcgtgttgcg cgaccaccag caggaccgcg ctctccacac ccgcctcgac gcggtccttc
     2281 acgccgtcga ttcggtaacc ggtctcggtg cgcgtcgcgg tgaccgaggt gttcaacggc
     2341 gcccacggct tgcctggctc gtagaccgcc cacgacgcca ccgcctcgcc cgatatcagc
     2401 gattcgattg tgtcctgatg gttttcgggg gcctcgacca aacctgccag cacggtgctg
     2461 accggatgca gcggtccagg cgcgacggtc ctgcctgcca gctcggcgac cagggcgagg
     2521 tccgccacgc catcgccgga cacgctgccg ccgccgagat cttcgggcac cagcaggctc
     2581 gtccagccga gctcggcggc ccgttgccac cagcccgcct caaaggacgc accggccgca
     2641 tggagttcac gcagccgggc tagcgaagct tccttctcca gaaacgactg tgtcgtcgag
     2701 gcgaacagca gccgttcagg attcgcgtct tcggtcatgt agttcaggcg ggaatgacga
     2761 gaggtggcac gtcggggttg tggacgatct tgttctccac cttgaacagg tcgatcatgt
     2821 tgccgcccat gacctttcgc tgaccctcct catcgagacc ctggagatcg tcgatcagat
     2881 gggtcggctc ggccagcccc tcgggatgcg gccagtcgga accgaagatc acccggtcga
     2941 cgccgcacag gccggccatc tgcgcgaagt cgtcctccca gaatggtgcc acgtagacgc
     3001 cacgcttgaa ggcctcgatc gggttctccg ggaacgcctg cggcatcttc ttgtaggtgt
     3061 t
//
LOCUS       DASRJA010000080         6375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_497948,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000080 DASRJA010000000
VERSION     DASRJA010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497948"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..771)
                     /gene="fabG1"
                     /locus_tag="VFC01_03670"
     CDS             complement(4..771)
                     /gene="fabG1"
                     /locus_tag="VFC01_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG1"
                     /protein_id="HZN78777.1"
                     /translation="MPVTDDPAEAAGETAAGRPAFVSRSVLVTGGNRGIGLAIAQRLA
                     ADGHKVAVTHRGSGAPDGLFGVECDVTDNEAVDRAFKEVEEHQGPVEVLVSNAGLSAD
                     AFLIRMTEERFEKVIDANLTGAFRVAQRASRSMQRKRFGRMIFIGSVSGTWGIGNQAN
                     YAASKAGVIGMARSIARELSKAGVTANVVAPGYIDTDMTRALDERIQDGALEFIPAKR
                     IGTAAEVAGVVSFLASEDASYISGAVIPVDGGMGMGH"
     gene            complement(815..1822)
                     /locus_tag="VFC01_03675"
     CDS             complement(815..1822)
                     /locus_tag="VFC01_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN78778.1"
                     /translation="MTLPVLGPMTLTGFDHPWFFLFLFVVLGLVGLYIAMQVARRKRM
                     LRFANTELLESVAPKRPSRWRHLATILLVLSLVLFTVAMAGPTRDVRIPRNRAVVMLV
                     IDVSQSMRATDVAPSRMAAAQEAAKQFVDQLTPGVNLGLISYAGAATVLVSPTTNRDA
                     SKNAIDKLQVADRTATGEAIFTALQAINTVGAVIGGGDEPPPARIVLMSDGKETTPSN
                     PDNPKGAYTAARTAKDQGVPISTVAFGTPNGYVEINDQRQPVPVDDEMLKKIAQLSNG
                     QAYKASNVEQLKEVFTNLQEQIGYETRKGDASAGWLRLGALVLALAAVAALLINRRLP
                     N"
     gene            complement(1855..2862)
                     /locus_tag="VFC01_03680"
     CDS             complement(1855..2862)
                     /locus_tag="VFC01_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN78779.1"
                     /translation="MTIPLLGPMTLSGFEHAWFFLFLLVVFGIVALYVIVQVARHKRM
                     LRFANMELLESVAPKRPSRWRHLPAILLVLSLLLFTVAMAGPTHDVRIPRNRAVVMLV
                     IDVSQSMRATDVSPNRLAAAQEAAKQFADQLTPGINLGLIAYAGTATVLVSPTTNREA
                     SKAAIDKLQLADRTATGEGIFTALQAIATVGAVIGGGDEPPPARIVLMSDGKETVPSN
                     PDNPKGAYTAARTAKDQGVPISTVSFGTPYGYVEINDQRQPVPVDDEMLKKIAELSGG
                     QAYTASSLEQLKEVFTNLQEQIGYETRKGDASASWLRLGAVVLALAALAAMLINRRLP
                     N"
     gene            complement(2886..3833)
                     /locus_tag="VFC01_03685"
     CDS             complement(2886..3833)
                     /locus_tag="VFC01_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN78780.1"
                     /translation="MTTSRRTVDLPSLKRGEIRDPALTAALRKLELTVRRKLDGVLHG
                     DHLGLLPGPGSEPGESRLYQPGDDVRRMDWSVTARTTHPHVRQMIADRELETWLVVDM
                     SASLDFGTTGCEKRDLAVAAAAAITFLNSGGGNRIGAIIANGDSVRRVPALSGRMHEQ
                     ELLRTIATMPKAPTGVRGDLAAAIDALRRPERRRGMAVILSDFLGPINWMRPLRAIAG
                     RHEVLGIEILDPRDVELPPVGDVILQDTETGVTREFTIDEQLRDDFEKAAAAHRAEVA
                     RTLRRCDAPLLSLRTDRDWIADVVRFVATRRRGALAGRP"
     gene            complement(3830..4981)
                     /locus_tag="VFC01_03690"
     CDS             complement(3830..4981)
                     /locus_tag="VFC01_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN78781.1"
                     /translation="MTSPSGPPQGAGGYSGQAPTQGYNPPGNPGSHTGSPNNGGLQQE
                     VHTLERAIFEVKRIIVGQDQLVERMLVGLLAKGHVLLEGVPGVAKTLAVETFAKVVGG
                     TFARIQFTPDLVPTDIVGTRIYRQGKEEFDIELGPVVVNFLLADEINRAPAKVQSALL
                     EVMAERKISIGGKTFPLPAPFLVMATQNPIEQEGVYALPEAQRDRFLFKLNIDYPTPE
                     EEREIVYRMGVKPPEPKQILNPGDLLRLQDVASNTFVHHALVDYVVRVVTATRHPEKF
                     GMPDAKAWIAYGASPRASLGIIAASRALALVRGRDYVIPQDVVEVIPDVLRHRLVLTY
                     DALADEISPETVVNRILQTVALPQVNAIPQQGHSVPPVMQTAAAAAGNR"
     gene            complement(5156..5854)
                     /gene="ripB"
                     /locus_tag="VFC01_03695"
     CDS             complement(5156..5854)
                     /gene="ripB"
                     /locus_tag="VFC01_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family peptidoglycan endopeptidase
                     RipB"
                     /protein_id="HZN78782.1"
                     /translation="MVILLRRFTIALLVFAGLLLGLSAPALADPDDGQWDPTLPKLIS
                     SGAPGDPLAIANASLAATTQATQVTMDLGRKFLSSLGLAPPAASSAGVAPGRVRGPQA
                     IEYVIRRGASQMGVPYSWGGGKPTGPTTGIDSGANTVGFDCSGFTQFSFAGVGVLIPK
                     YSGDQYNTGRKVPVSQAKRGDLLFWGPGGSQHVALYLGGGQMLEASGSADKVTVSPVR
                     RGGLQPYAARIIES"
     gene            complement(5848..>6375)
                     /locus_tag="VFC01_03700"
     CDS             complement(5848..>6375)
                     /locus_tag="VFC01_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HZN78783.1"
                     /translation="ILGYASTSAQVTQQMGRNFLQSVGLLPTPSGYTNGAIPRVYGRQ
                     ATEYVINRGLAVRGTPYSWGGGNAAGASRGIDSGAGTVGFDCSGLMLYMFAGVGIKLD
                     HYSGSQYNAGRKVPSSQMRRGDMIFYGPNASQHVAMYLGDGQMLEAPYTGSVVKVSPV
                     RTSGMTPYVTRLIEW"
BASE COUNT         1060 a         2114 c         2088 g         1113 t
ORIGIN      
        1 ctcttagtgg cccatgccca tgccaccgtc gaccgggatc accgcaccgg agatgtaact
       61 cgcatcctcg gacgccagga agctgaccac cccggcgacc tcggcggcgg tgccaatccg
      121 cttcgccggg atgaactcca gtgccccgtc ctgaatcctt tcatccagcg cgcgggtcat
      181 atcggtgtcg atgtagcccg gagccaccac attcgcggtg accccggcct tggacagctc
      241 tcgggcgatc gagcgggcca tgccgataac accggccttg gaggccgcgt aattggcctg
      301 gttgccgatg ccccaggtgc cggagaccga acctatgaag atcatccggc cgaatcgctt
      361 tcgctgcatg ctgcgcgatg cccgctgggc cacccggaac gccccggtga ggttcgcgtc
      421 gatgaccttt tcgaatcttt cttccgtcat ccggatgagg aatgcatcag cggacaggcc
      481 ggcgttggac accagcacct cgaccgggcc ctgatgctcc tcgacctctt tgaaagcgcg
      541 gtcgacggcc tcgttgtcgg tcacatcgca ctcgaccccg aacagcccgt cgggcgcgcc
      601 cgatccgcgg tgggtgacgg cgaccttgtg gccgtcggcg gccaggcgtt gggcgatggc
      661 caggccgatg ccgcggtttc cgccggtcac cagtaccgac cgcgatacga aggccggacg
      721 gcccgccgcg gtttctcccg cggcctcggc cggatcgtca gtcacaggca tgcccgccaa
      781 cttagtgcct aagccgcatt cttaagaaat cgccttagtt gggcagccgc cggttgatca
      841 acaacgcggc taccgccgcc aacgccagca ccaatgcgcc gagccgcagc cagcctgcgc
      901 tggcgtcgcc cttgcgagtt tcgtagccga tctgctcctg caggttggtg aagacttcct
      961 tgagctgctc gacgttggac gccttgtagg cctgcccatt ggacagctgg gcgattttct
     1021 tgagcatctc gtcgtcgacg ggcacgggct ggcgctggtc gttgatctcg acgtagccgt
     1081 tcggggtgcc gaacgccaca gtcgagatcg gcacgccctg gtccttggcc gtgcgcgcgg
     1141 cggtgtatgc gcccttgggg ttgtccgggt tcgacggcgt ggtctccttg ccgtccgaca
     1201 tcagcacgat gcgggccggc ggcggctcgt ccccaccacc gatcaccgca ccgacggtgt
     1261 tgatcgcctg cagtgccgtg aagatggctt caccggtcgc ggtgcgatcg gccacctgca
     1321 gcttgtcgat cgcgttcttg gacgcatcac ggttggtggt cggcgagacc aacacggtcg
     1381 cggcgcccgc gtaggagatc aacccgaggt tgacccccgg cgtcagctga tcgacaaact
     1441 gcttggcagc ctcctgcgcc gccgccatcc gactcggcgc aacgtcggtt gcccgcatcg
     1501 attgcgacac gtcgatcacc agcatcacca ccgcgcggtt acgaggaatc cggacgtctc
     1561 gggtcggccc ggccatcgcg acggtgaaca gcaccagcga caacaccaac aatatcgtcg
     1621 ccagatgccg ccagcgcgac ggccgtttgg gcgcaacgct ttccagcagc tcggtgttgg
     1681 caaagcgcag catccgcttg cgtcgggcca cctgcatcgc gatgtagagc ccgaccagcc
     1741 ccagcacgac aaagaggaac aggaagaacc aggggtggtc aaaccccgtc agcgtcatcg
     1801 gtccaagaac cggtaatgtc atgtgctgct tgccatttca gttgctcttg ggcgttagtt
     1861 tggtagtcgg cggttgatta gcatggccgc cagtgccgcc agggccagca ccactgcgcc
     1921 aagccgtagc cagctggcgc tggcgtcgcc cttgcgggtt tcgtaaccga tctgctcctg
     1981 caggttggtg aaaacctcct tgagttgctc caggctcgat gcggtgtagg cctgaccgcc
     2041 ggacagttcg gcgatcttct tgagcatctc gtcgtcgacg ggcacaggct ggcgctgatc
     2101 gttgatctcg acgtagccgt acggcgtacc gaacgacacc gtcgagatcg gcacgccctg
     2161 atccttggcc gtgcgcgcgg cggtatacgc gcctttgggg ttgtcgggat tcgacggcac
     2221 cgtctccttg ccgtccgaca tcagcacaat gcgggccggc ggcggctcgt ccccaccgcc
     2281 gatcactgcg cccaccgtgg cgatcgcctg cagcgcggtg aagatgccct caccggtcgc
     2341 ggtgcggtcg gccaactgaa gcttgtcaat tgcggccttg ctcgcctcac gattggtggt
     2401 cggcgacacc agtacggtcg cagtgccggc gtaggcgatc agccccaggt tgatccccgg
     2461 tgtcagttga tcggcgaact gcttggctgc ctcttgggcc gcggcgaggc ggttcgggga
     2521 cacgtcggtg gcgcgcatcg attgcgacac gtcgatcacc agcatcacca ccgcgcggtt
     2581 gcgtggaatc cggacgtcat gggtgggccc ggccatcgcg accgtgaaca gcagcagcga
     2641 cagcaccagc aggatcgcgg gcaggtgccg ccaccgcgac ggccgtttgg gcgcaacgct
     2701 ttccagcagc tccatgttcg caaagcgcag catgcgcttg tgtctggcca cctgaacgat
     2761 gacgtacagc gcgacgattc cgaagacaac gaggaggaac agaaagaacc acgcgtgttc
     2821 gaaaccggac agcgtcatcg gtccgagcaa cggtattgtc atgtgctagt tgtcatttcg
     2881 ttggatcagg gccgtccggc aagcgctcca cgtcggcggg tggcgacaaa gcggacaaca
     2941 tcggcgatcc agtctcggtc tgtgcgcagc gacagcaacg gtgcgtcgca gcgccgcagc
     3001 gtgcgggcca cctccgcccg gtgcgccgcc gccgccttct cgaagtcgtc gcgcagctgc
     3061 tcgtcaatgg tgaactctcg agtgacccca gtttcggtgt cctgcagaat gacatccccg
     3121 accggcggta attcgacgtc gcgcgggtcg agaatctcga tgccgagcac ctcatgccgt
     3181 cccgcaattg cccgcagcgg acgcatccag ttgatcggac cgaggaagtc gctgagaatc
     3241 acggccatgc cacggcgacg ctcgggcctg cgcagcgcgt caatcgcggc ggccaagtcg
     3301 ccgcgtacac ccgtcggcgc cttgggcatt gtggcgatcg tgcggagcag ttcctgctcg
     3361 tgcatgcggc ccgatagcgc cggcacccgg cgcacagagt cgccgttggc gataatcgcg
     3421 ccgatccggt tgccgccacc gctgttgagg aacgtgatcg ccgcagccgc cgctaccgcc
     3481 aggtcgcgct tttcacaacc ggtggtgccg aagtcgaggc tggccgacat atcaaccaca
     3541 agccatgtct cgagctcacg gtcggcgatc atctggcgca cgtgcggatg ggtggtgcgt
     3601 gcagtgaccg accagtccat tcgtcgcacg tcgtcgccgg gctgatataa ccgcgactcc
     3661 cccggctcgg agcccggccc cggcagcagc ccgaggtggt cgccgtgcag cacgccgtcg
     3721 agcttgcggc gcaccgtcag ctcgagcttg cgcagcgccg cggtcagcgc cggatcacgg
     3781 atctccccgc gcttcagcga cggcaggtcg accgtgcgtc tggaagtagt caccgattac
     3841 cggcggcagc cgctgcggtt tgcatgaccg gcggcaccga atgtccttgc tgcggaatgg
     3901 cattcacctg cggcagggcc accgtctgca ggatccggtt gacgaccgtc tcgggtgaga
     3961 tctcgtcggc cagtgcgtcg taggtcagca cgagccggtg acgcagcaca tcgggaatga
     4021 cctcgacgac gtcctgcggg atcacgtagt cacgcccacg caccaacgcc agcgcgcgcg
     4081 acgccgcgat gatgccgagc gaggcacgcg gcgaggcacc gtaggcgatc catgccttgg
     4141 cgtcgggcat gccgaatttc tcggggtgcc gggtcgcggt gacgacgcgc accacgtagt
     4201 cgaccagagc gtggtgcacg aacgtgttcg acgcgacgtc ctgcagacgc agcaggtcac
     4261 cgggattgag gatctgcttc ggctccggcg gcttgacgcc catccggtag acgatctcgc
     4321 gctcctcctc cggcgtgggg taatcgatgt tcagcttgaa caggaagcgg tcgcgctgcg
     4381 cttccggcag tgcgtagacg ccttcctgct cgatcgggtt ctgcgtcgcc atcacgagga
     4441 acggcgccgg cagcgggaac gtcttaccgc cgatcgagat cttgcgctcg gccatcacct
     4501 cgagcagtgc cgactgcacc ttggcgggtg cgcggttgat ctcgtcggcg agcaggaagt
     4561 tcaccaccac gggcccgagt tcgatgtcga actcctcctt gccctggcgg tagatccggg
     4621 tgccgacgat gtcggtgggc accaagtcag gagtgaactg gatgcgggcg aaggtgccgc
     4681 cgaccacctt ggcgaaggtt tcgacggcca gggtcttggc cacccctggc acaccttcga
     4741 ggagcacgtg gcccttggcg agcaggccga ccagcatccg ctcgaccagt tggtcctggc
     4801 cgacgatgat ccgcttgacc tcgaagatgg cgcgctcgag ggtgtgaacc tcctgctgca
     4861 ggcctccgtt gttcggcgat ccggtgtggg agccggggtt accgggaggg ttgtagccct
     4921 gggtcggggc ctgtccggaa tatcctccag cgccctgcgg gggcccactc ggtgacgtca
     4981 tcaacggtcc ttccacatct attaagacgc tggtcgtagc ggcgtcggcc gaagttggcc
     5041 gcacgctcgc cgtcaactat tccaggcact ccggaatccg tcgacgttgc ccagcgaagt
     5101 gtcggctggt cgaacggcat tcatcaggag tgctcaggat taactcaggt cccgctcagg
     5161 attcgataat tcgggcggca tacggctgca gaccaccccg ccgcaccggg ctgaccgtga
     5221 ctttgtctgc gcttcccgag gcctccagca tctgaccgcc gcccagatat agcgcgacgt
     5281 gctggctacc gccgggaccc cagaacaaca ggtcgccgcg cttggcttgc gacacaggca
     5341 ccttgcgccc ggtgttgtac tgatcaccgg agtacttcgg gatcagcacg ccgacgccgg
     5401 cgaacgagaa ctgggtgaag cccgagcagt cgaagccgac ggtgttggcg ccggagtcga
     5461 tgccggtggt gggcccggtg ggcttgccgc cgccccacga gtacggcaca cccatctgcg
     5521 aggcgccgcg acggatcaca tactcgatgg cctgcggtcc gcgcacccgg cccggcgcga
     5581 cgccggcgct cgacgcagct ggcggcgcaa gtcccaagct gctcaagaac tttcggccaa
     5641 ggtccatggt gacctgggtg gcctgggtgg tcgcggcgag cgacgcgttg gcgatcgcca
     5701 gcgggtcacc aggcgcgcca gagctgatca gcttgggcag cgtcggatcc cattgcccgt
     5761 catcggggtc ggccaatgca ggcgcggaca gcccgagcag cagcccggcg aacaccaata
     5821 gtgcaattgt gaagcggcgc agaagaatca ccattcaatc aatcgggtca cgtagggggt
     5881 catgccgctg gtgcgcaccg gcgagacctt gaccacggag ccggtgtacg gagcctcgag
     5941 catctggccg tcgccgaggt acatcgcgac gtgctggctg gcgttggggc cgtagaagat
     6001 catgtcgccg cgacgcatct gcgaggacgg aaccttgcgg cccgcgttgt actgcgagcc
     6061 cgagtagtgg tcgagcttga tgccgacgcc ggcgaacatg tacagcatca gcccggaaca
     6121 gtcgaaaccg actgtgccag ccccggaatc gatgccgcgg ctggcgccgg ccgcgttgcc
     6181 gccaccccat gagtacggcg tgcccctcac ggcgaggccg cggttgatga cgtactcggt
     6241 ggcctgcctg ccatagaccc ggggaatcgc gccgttggtg tagccgctcg gcgtcggcag
     6301 gagcccgacc gactgcagga agttgcggcc catctgctgc gtcacctgcg ccgacgtcga
     6361 cgcgtagccc aggat
//
LOCUS       DASRJA010000081         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1897954,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000081 DASRJA010000000
VERSION     DASRJA010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897954"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VFC01_03705"
     CDS             complement(<1..750)
                     /locus_tag="VFC01_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZN78784.1"
                     /translation="MADDPGTGQAVQAPPEAQLSETQPLEIPKVSTSASRRVRARLAR
                     RMTAQRSAVNPVLEPLVAVHKGHFPKANLALLQKAYEVAEERHADQMRRSGDPYITHP
                     LAVANILAELGMDTTTLVAALLHDTVEDTGYTMEQLTADFGEEVAHLVDGVTKLDRVA
                     LGTAADAETIRKMIIAMARDGRVLVIKVADRLHNMRTMRFLPPEKQARKARETLEVIA
                     PLAHRLGMATVKWELEDLSFAILHPKKYEEIV"
     gene            complement(791..1315)
                     /locus_tag="VFC01_03710"
     CDS             complement(791..1315)
                     /locus_tag="VFC01_03710"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HZN78785.1"
                     /translation="MSDISRLIASLTREVADFPEPGIQFKDLTPLLADAHGLDAVTDA
                     LADVARGADLVAGVDARGFLLGGAVAIKLGIGVLAVRKGGKLPPPVIGETYDLEYGTA
                     TLEVPAEGIELAGRNIAIIDDVLATGGTVAATAKLLRHAGANVTGAAVVLELTALGGR
                     DVLKPLPVSSLHTV"
     gene            complement(1312..2922)
                     /locus_tag="VFC01_03715"
     CDS             complement(1312..2922)
                     /locus_tag="VFC01_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN78786.1"
                     /translation="MLSLIGGLGLSACSGSPADSIDYAVDGTLVSYNTNTVAGAASGG
                     PQAFARVLTGFNYHGPDGQIVGDHDFGTISVVGRAPLVLDYEIGANAVYSDGKPITCD
                     DMVLAWASQSGRLPGFDAASRAGYSDIAAVDCTPGQKKARVTFVQDRGFVDFGQLFAA
                     TSMMPSHVIADELGIDVTTALLNNDGPTVERIAQVWNNTWNLDAEHFDAKRFPSSGPY
                     KLDAVNKDGSVVLVANDKWWGAKPVTQKITVFPRGADIQDKVNQGAYDVVDVATGSSG
                     TLNLPDDYVRTDSPSAGIEQLIFAPEGPLAGPPARRALALCTPRDVIAKNAAVPIANA
                     RLNPASEDAFGATEGAVEAGQFVVANPDAARAAINNQPMTVRIGYQSPNARLAATVGA
                     ISKACAPAGITVQDAAADTVGPLSLRGNKIDVLIASTGGAAGGGSSGSSAMDAYDLHT
                     GNGNNLSGYSNERIDGIIASMAVTSDPKEIARLLGEGGSILWADMPTLPLYRQQRTLL
                     TSKKMYAVSSNPTRWGAGWNMDRWVLSQ"
BASE COUNT          442 a          987 c          980 g          524 t
ORIGIN      
        1 cacgatctcc tcgtacttct tcggatgcag aatcgcgaac gacaggtcct cgagctccca
       61 cttcaccgtg gccatgccca gtcgatgtgc cagtggcgca atgacttcca gcgtctcgcg
      121 ggcctttcgg gcctgctttt ccgggggcag gaagcgcatg gtgcgcatgt tgtgcagccg
      181 gtcggcgacc ttgatcacca gcacccgccc gtcgcgggcc atcgcaatga tcatcttgcg
      241 gattgtctcg gcgtcggcgg cggtgcccag cgctacccgg tccagcttgg ttacgccgtc
      301 aaccaggtgc gcgacctcct cgccgaagtc ggcggtcagc tgttccattg tgtagccggt
      361 gtcctcgacg gtgtcgtgca acagcgcggc gaccagcgtg gtggtgtcca tgccgagctc
      421 ggccaggatg ttggcgacgg ccaacgggtg ggtgatgtac ggatcgccgg agcgacgcat
      481 ctggtcggcg tgccgctcct cagctacctc gtacgccttc tgcagcaacg ccaggttggc
      541 cttcgggaag tgtcccttgt gcactgcgac gagcggctcg agcaccgggt tgacggcgct
      601 gcgctgggcg gtcatccggc gggccagccg ggcccgtacc cgccgcgacg cgctggtcga
      661 aaccttgggg atctccagcg gctgcgtttc ggagagctgc gcttctggag gcgcctgcac
      721 ggcctgtccc gtgccgggat cgtcagccat ggtcacctcc taccatttcg ccagacttcg
      781 aggatatccc tcacaccgtg tgcaggctgc tcaccggtaa cggtttgagc acgtcacggc
      841 cgcctagcgc ggtcagttcc agcaccacag cggcgccggt gacgttggcg cccgcatgcc
      901 gcagcagctt cgccgtcgcg gctaccgtgc cgccggtagc tagaacgtcg tcgataatcg
      961 cgatgttccg gccggccaac tcgatgccct cggccggcac ctccagcgtg gcagtgccgt
     1021 attccagatc gtaggtttcg ccgatcaccg gtggtggcaa cttgccgccc ttgcggaccg
     1081 ccagcacacc gatgcccagt ttgatggcga cggcaccgcc gagcaggaag ccgcgggcgt
     1141 ccacgccggc caccaggtcg gcgcccctgg cgacgtcggc aagcgcgtcg gtcaccgcgt
     1201 ccaggccatg cgcgtcggcc agcagtgggg tgaggtcttt gaactggatg cccggctcgg
     1261 gaaagtcggc gacctcacgc gtcagcgacg cgatcagccg cgagatgtcg ctcattgcga
     1321 cagcacccag cggtccatgt tccaccccgc gccccatcga gtcggattgc tgctcacggc
     1381 atacatcttc ttcgaagtca gcagggttcg ctgctggcgg tatagcggaa gggtcggcat
     1441 gtcggcccac aggatcgatc cgccctcccc gagcaaccgg gcgatctcct tcgggtcgga
     1501 ggtcaccgcc atcgacgcga tgattccgtc gatgcgctcg ttggagtacc ccgacaggtt
     1561 gttgccgttg ccggtgtgca gatcgtaggc gtccatcgct gatgagccgc tggacccacc
     1621 gcccgctgca ccgccggtgc tggcgatcag cacgtcaatc ttgttgccgc gcaggctcaa
     1681 cggaccgacc gtgtcagccg cggcatcctg cacggtgatt ccggccggcg cacaggcctt
     1741 ggagatcgcg ccgaccgtcg cggcaagccg ggcgttcggg ctctgataac cgatgcgcac
     1801 ggtcatcggt tgattgttga tcgcggcgcg ggccgcatcg gggttggcga cgacgaactg
     1861 tccagcctcg acggcgccct ccgtggcgcc gaacgcatcc tcgctggccg ggttgagtcg
     1921 ggcgttggcg atcggcactg ccgcgttctt ggcgatcaca tcccggggcg tgcacagcgc
     1981 aagcgcccgg cgcgcgggcg ggccggccaa cgggccctcc ggcgcgaaga tgagttgttc
     2041 gatgccggcc gacggcgagt cggtgcggac gtagtcgtcg ggcagattca gcgttcccga
     2101 cgaaccggtc gccacgtcga cgacgtcata ggcaccctgg ttgaccttgt cctggatgtc
     2161 ggcgccgcgc ggaaacacgg tgatcttctg ggtgacgggc ttggcgcccc accacttgtc
     2221 attggccacg agcacgaccg agccgtcctt gttcaccgcg tcgagcttgt acggccccga
     2281 cgatgggaac cgtttggcgt caaagtgctc cgcatcgaga ttccaggtgt tgttccacac
     2341 ctgcgcgatg cgttcgacag tcgggccgtc attgttgagc agcgcggtgg tgacatcgat
     2401 gccgagttcg tcggcgatga cgtgcgacgg catcatcgac gtcgcggcga acagctggcc
     2461 gaagtccacg aacccccggt cctggacgaa agtcacccgc gccttcttct gtcccggcgt
     2521 gcagtcgacg gcggcgatgt cgctgtagcc ggcgcggctg gccgcgtcga acccaggcag
     2581 ccgacccgac tgcgatgccc acgccaggac catgtcgtca cacgtgatcg gcttgccgtc
     2641 ggagtacacc gcgttggcgc cgatctcgta gtcgagcacc agcggtgcac gcccgaccac
     2701 cgaaatggtg ccgaagtcgt gatcgccgac gatttggccg tcgggtccgt ggtagttgaa
     2761 gccggtcagc acccgggcga aggcctgcgg accacccgat gcagcgccgg cgacggtgtt
     2821 ggtgttgtag ctgaccagcg tgccgtcgac ggcgtagtcg atgctgtcgg ccgggctgcc
     2881 cgagcatgcc gagagaccca gcccgccgat cagcgacagc acggccgccg cga
//
LOCUS       DASRJA010000082         4428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1622374,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000082 DASRJA010000000
VERSION     DASRJA010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622374"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            505..2388
                     /locus_tag="VFC01_03720"
     CDS             505..2388
                     /locus_tag="VFC01_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HZN78787.1"
                     /translation="MSVYQRSDVPFANNLFTLPLTPDYWTSVLTSNFPTLLGGVLGGV
                     ITNPNLNLPDGSILTLQGTFTVALGLGTADISGTVTGLVRTAAGMIIETVSGFSLPFT
                     AHYDAFNSANNSSSAPTLPDIINGDDQLNGGSQDDLLMGFGGADTFNGGAGTDTVSYV
                     AAPTAVRADLGNPATNTGSDAVGDTYSSIENLIGSQFNDTLVGNAQDNVLTGGLGTDS
                     MLGGLGNDSYVVDNIGDVVTENLNQGSDTVHASIDYRLTANVEYLVLQGGAVQGYGNA
                     LSNAIYGTGADNLLDGDTGADAMYGGAGSDSYFLDNISDSISENANEGSDTVYASANY
                     RLGANVEYLVLQGSAVQGYGNSLTNVISGTSADNLLDGDAGADAMYGGAGNDSYFLDN
                     INDQVVENLNAGSDTVYSTANYRLGANVEYLVLQGGAVQGYGNSLTNVISGTSADNLL
                     DGDAGADAMYGGAGNDSYFVDHINDAVVENLNEGSSDTVYASVDYRLGANVENLVLQG
                     AAVQGYGNALANALSGTSNGNLLNGGGGGDFLTGGGGNDFFAFNTGEANGDIVADFAG
                     GGTGAGDSLLFVGYGTTVQGATFTRIGMTNQWQIHSGLDGHNEIITFSNGAAIDPTDY
                     SFM"
     gene            2525..>4428
                     /locus_tag="VFC01_03725"
     CDS             2525..>4428
                     /locus_tag="VFC01_03725"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HZN78788.1"
                     /translation="MADIPGNITTQRTLRVGDTWSDAIELSTDQDWFRVWLVQGVTYH
                     IRLDSSGVTPLIDPVLRVLDGTGTVELAFDDAGGGGLNSLIDMTAAVTGTYFISAQGF
                     GSSTGQYTLGLTETDIEPAPTVANSISGTSTTAVVLADGFVRGTLSNITYFDPLLNDY
                     FTRLDDDWYRIDVQAGQEYNFVWARAGAGGATDVNLTLYDAAGNVRFPLQNPGTWDWS
                     YIHWFPDSSGTWFIGVSGAATSGEYTLGVNRITDSPIESISGATALRFKQVDVYFAQA
                     GETFDGTTSEGWNAFEIAQAMAALGEYSKIANVTFSQTMVQADAEFTLVLDDNEQLEF
                     AGKFGLPGEPIEGIGTFDGNWAGWSTTAGGAVPGEAIDQGGYGFDTLLHEFGHGLGLV
                     HPHEDSYGNKIMHGVNDSEDHGDFDLNQGIYTVMSYNTGWETAPQGEPTKPDGKLDAR
                     FGWTGTPMAFDIAVIQQKYGANTGYHTGNDTYTLPSADGSGTFYSCIWDAGGIDTIRA
                     GATTASCIIDLRAATLRYELGGGGWVSYQAGIHGGFTIANGVVIENAIGGAGGDQITG
                     NAANNVLDGAVGADSMIGGAGNDVYFVDNAADVATENVGEGNDTVYSTAHFRLSANVE
                     GLILQGSADL"
BASE COUNT          883 a         1417 c         1373 g          755 t
ORIGIN      
        1 tgagctcggg cgcgtccgtt gccggcgcaa cgtgcgtcga ttccagcgcg cgcaccggcg
       61 cgtccgcatg ccgccggaac aaccacgatt cagaactatt cacatctgcc ccaagaactc
      121 ggccaagtgc gccggtgcac gctgcagagt atggcggctt cccaccgccg cagcgactcc
      181 tgcgggacgg agatgcaacc cgcagaattc gatcgtatcg cgtaagcgta tggaggcacg
      241 cgatgcccct tggcgatcag cagagcagag acgacgccat cgtcgtcgac gccacaggaa
      301 ctgacagtgg catccaataa tgtccttcgc ttgtgaccga cctcacaccc cgacggaagg
      361 cgacgcggct atgtagtgcg tgcatcttgc ttctcatcgg cacggcgcgt gcgcaatggc
      421 tggggtcgcc gccgagctcc ttggccacac cgttctgccg gatttgaggt cgattcctgc
      481 ctgcaggcca atggaggctc gacgatgtcg gtataccagc gctccgacgt gccgtttgcg
      541 aacaacctgt tcacgctgcc ccttacgccg gattactgga cttcggtact aaccagcaat
      601 ttcccgaccc tgcttggtgg agtattgggc ggggtcatca ccaatcccaa tctgaacctg
      661 cccgatggaa gcatcctgac gctccagggc acgtttacgg tggcgctagg gctcggaacc
      721 gccgacattt ccggcaccgt caccggcttg gtccgtacgg ccgccggcat gatcatcgaa
      781 acggtgagcg ggttttcgct gcccttcacc gcccactacg acgccttcaa ctctgcaaat
      841 aattcgtcct cggcgccgac cctcccggac atcatcaacg gcgacgacca gctcaacggc
      901 ggcagccaag acgatctgct gatggggttc ggcggcgccg acaccttcaa tggcggcgcc
      961 ggtacggaca ctgtgtccta cgtcgcggcc ccgacggcag tccgcgccga cctgggcaat
     1021 cctgcgacca acaccggctc ggatgctgtc ggcgacacct acagctccat cgagaacctg
     1081 atcggctccc agttcaacga caccctggtc ggaaacgctc aggacaacgt gctcaccggc
     1141 ggtctgggca ccgacagcat gctcggcgga ttgggcaatg actcctatgt cgtcgacaac
     1201 attggcgacg tggtgaccga gaatctcaac cagggcagcg acacggtcca tgcgtcaatc
     1261 gattacagac tcacggccaa cgtggaatac cttgtcctgc aagggggcgc cgtgcagggc
     1321 tacggcaacg ccctgtccaa tgcgatctac ggcaccggcg cggacaacct gctcgacggc
     1381 gataccggcg ccgacgccat gtatggcggg gccggcagcg actcgtattt cctcgacaac
     1441 atcagcgatt cgatcagtga gaacgccaac gaaggcagcg acacggtcta tgcgtcggcc
     1501 aactatcggc tcggggcgaa cgtggaatat ctggtgctgc agggcagcgc cgtgcagggc
     1561 tacggcaaca gcctgacgaa cgtgatcagc ggcaccagcg ccgacaacct gctcgacggc
     1621 gatgccggcg ccgacgccat gtatggcggg gccggcaacg actcgtattt cctcgacaac
     1681 atcaacgatc aggtggtcga gaacctcaat gcgggtagcg atacggtcta ctcgacggcc
     1741 aactaccggc tgggggcgaa cgtggaatac ctggttttgc aagggggcgc cgtgcagggc
     1801 tacggcaaca gcctgacgaa cgtgatcagc ggcaccagcg cggacaacct gctcgacggc
     1861 gatgccggcg ccgacgccat gtatggcggg gccggcaacg attcgtattt cgtcgaccac
     1921 atcaacgatg cggtggttga gaacctcaat gagggtagca gcgatacggt ctatgcgtcg
     1981 gtcgactacc ggctgggggc gaacgtggag aatctcgtgc tgcaaggagc cgccgtgcag
     2041 ggctatggca acgctctggc gaacgcgctc agcggcacct ccaatggcaa cctcctcaat
     2101 ggcggcggag gtggcgactt tctcaccggc ggcggcggca atgatttctt cgcgttcaac
     2161 accggggaag ccaacggcga catcgtcgcc gactttgctg gcggcggcac cggcgccggg
     2221 gattccctcc tgttcgtcgg ctacggcacc accgtgcaag gggccacatt cacccggatt
     2281 ggcatgacca atcaatggca gatccactcc ggcctcgacg gccacaacga gatcatcacc
     2341 ttcagcaacg gggcggcgat cgatccgacc gactacagct tcatgtgaag ccgcaatacg
     2401 gccagatgac cgtctgtaac aaaatcgagc tcacgtctgc tcgcccatcg gcgcctgatg
     2461 gagcagccga agatgccgtc tgggcattgt cgagcactgc tcatggcgcg tggaggaccg
     2521 aacgatggct gacatcccgg ggaacataac cacccagcgc acgcttcgcg tcggtgacac
     2581 gtggagcgat gccatcgagc tgagcaccga ccaagactgg tttcgggtgt ggttggtcca
     2641 gggagtgacc tatcatatca gactcgacag ctcgggtgtt acgccgctca tcgatccggt
     2701 ccttcgcgtg ctggacggca ccgggactgt tgaactcgcc ttcgacgacg ccggaggtgg
     2761 cggcctcaac tcgctgatag acatgactgc agccgtcacc gggacgtact tcatctcggc
     2821 gcaggggttc ggaagttcta cgggccagta cacgctgggg ctcaccgaaa cagacatcga
     2881 gcctgcccct acggtggcca attcgatctc tggaacgtcc acgacggccg tcgtactggc
     2941 cgacggtttc gtccgcggga cgctgagcaa catcacatac tttgatcccc tgctgaacga
     3001 ctacttcacc cgtctggacg acgactggta ccggatcgat gtccaggccg ggcaggaata
     3061 caacttcgtc tgggcgcggg ccggcgccgg tggggccacc gacgtcaatc tcaccttgta
     3121 cgatgcggca ggcaacgttc gctttccgtt gcagaacccc ggcacctggg attggagtta
     3181 catccattgg ttcccggaca gcagcggcac ctggttcatc ggcgtcagcg gcgcggctac
     3241 ttcgggcgaa tacaccctgg gcgtgaatcg catcaccgac tccccgatcg aatccatcag
     3301 cggggcgacg gcgctgcggt tcaagcaggt cgacgtctat ttcgcccagg ctggagagac
     3361 cttcgacggt acgacgtccg aaggctggaa cgctttcgaa atcgcccagg cgatggcggc
     3421 gctcggcgag tactccaaga tcgccaacgt cacgttcagc cagacgatgg tgcaggctga
     3481 cgcggagttc acgctggtcc tcgacgacaa cgaacaactg gagtttgccg gcaagttcgg
     3541 ccttcccggg gaaccgatcg agggcatcgg cacgttcgac ggcaactggg ccggctggtc
     3601 caccacggcc ggcggggcgg tgcccggcga ggcgatcgac cagggcgggt atggttttga
     3661 caccctgctg cacgagttcg gtcacggcct gggtctcgtc cacccgcacg aggattcgta
     3721 cggcaacaag atcatgcacg gagtgaacga ttcagaggac cacggcgact tcgacctcaa
     3781 tcagggcatc tacaccgtaa tgagctacaa cacaggctgg gagacggcac cgcagggcga
     3841 gccgaccaag cctgacggca agctcgacgc ccgctttgga tggaccggga cgcccatggc
     3901 gttcgacatc gcggtgatcc agcagaagta cggcgccaac accggctatc acaccggcaa
     3961 cgacacctac acgctgccga gcgccgacgg ctccggtacg ttctacagct gcatctggga
     4021 tgccggtggc atcgatacga tccgcgccgg cgccacgaca gcgagctgca tcatcgacct
     4081 tcgcgccgca acgctgcgat atgagctggg aggcggcggg tgggtctcgt accaggccgg
     4141 catccatggc ggcttcacga ttgccaacgg cgtcgtcatt gagaatgcaa tcggcggggc
     4201 aggcggcgac cagatcaccg gcaacgcggc caacaacgtg ctcgatggtg ccgttggcgc
     4261 cgacagcatg attggcggcg ccggaaacga cgtctatttc gtcgacaacg ccgccgacgt
     4321 ggcgaccgag aatgtcggcg agggcaatga cacggtctat tcgaccgccc acttcaggct
     4381 ttcggccaac gtggagggcc tgatcctgca aggcagcgcc gacctgca
//
LOCUS       DASRJA010000083        15725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2175852,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000083 DASRJA010000000
VERSION     DASRJA010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15725
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175852"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFC01_03730"
     CDS             complement(<1..252)
                     /locus_tag="VFC01_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN78789.1"
                     /translation="MTEDANPERLLFASTTQSFLEKEASLARLRELHAAGASFEAGWW
                     QRAAELGWTSLLVPEDLGGGSVSGDGVADLALVAELAGRT"
     gene            complement(261..1484)
                     /locus_tag="VFC01_03735"
     CDS             complement(261..1484)
                     /locus_tag="VFC01_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN78790.1"
                     /translation="MTRKLPYPVFDADNHFYEPKEALTKFLPDHRKNVIDYIEVRGRT
                     KIMVRNQVSDYIPNPTFEVVARPGAQEEYFRHGSGGKSFREVMGKPMKAIPAFREPAA
                     RLEVMDGLGLDYSLMFPTLASLVEERMKDDPELIHDVIHALNEWMYETWQFNYENRIF
                     STPVITLPIVDRALEELEWCLERGAKTVLVRPAPVPGMRGTRSFGTEEFDPFWDACVK
                     AGIPVSMHASDSGYAEYLNDWEPADEFLPFKPTAFRMVSMGKRPIEDSMAALVCHGAL
                     TRNPDLRVLSIENGADWVPYLFKQFENTYKKMPQAFPENPIEAFKRGVYVAPFWEDDF
                     AQMADLCGVDRVIFGSDWPHPEGLAEPTHLIDDLQGLDEEGQRKVMGGNMINLFKVEN
                     KIVHNPDVPPLVIPA"
     gene            complement(1596..2231)
                     /locus_tag="VFC01_03740"
     CDS             complement(1596..2231)
                     /locus_tag="VFC01_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78791.1"
                     /translation="MAKSTGRSPDSAGVDTEPRERRFMKSALAILGETGRTDFTVLEV
                     VERSKTSLRSFYQHFSTKDELLLALIDKIMSESTRKWREDTTGLESAAALRVLVDRIC
                     TPAESSTQDRVNRGLTDYNDHLAETLPREYARVLSPLHELIKDIINRGITEGAFRTDL
                     VVDATAALIMQSALGAMRLRVLGAELNGVPIDADHIYDFCISGLFRRDQAL"
     gene            2303..3199
                     /locus_tag="VFC01_03745"
     CDS             2303..3199
                     /locus_tag="VFC01_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HZN78792.1"
                     /translation="MTTDSAHPSSEAQWTVEGLLDLFDVQPDGENRFTAETGPAGEDQ
                     RQVVEGTQVLAQVIVAVAKRFPDKSLRSVYAVFARAVMVSAGPVELEIDVVSEGRSTA
                     TAIVAAKQNGKRCITATVLLDVPTGDVIRHHLPRPQVAAPADANVSEMPMTGRQLRLV
                     DVVDVNSPDEVGPPELYAWLHYDPIPTRDDLAKALIAYFTGHLGISTTMRAHEGIGTA
                     QSHLTVSTAPMTVSVSFHEPVRWDGWLLYTHESTQVGAGMSYVRGAVHTEKGELIASF
                     AQDALIRPLRTTDTAIKHQSRL"
     gene            complement(3196..4020)
                     /locus_tag="VFC01_03750"
     CDS             complement(3196..4020)
                     /locus_tag="VFC01_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZN78793.1"
                     /translation="MAEDKPLAGKVAYVTGAARGQGRSHCIRLARAGADIVAIDACAP
                     VTDYNGYPPATPEDLAETVSLVEGEGRKILADEVDVRDAAGQQRVVADALEQFGRLDI
                     VVANAGVLNWGRLWEISAQQWQDILDINLTGLWNTIKAVVPAMIEAGNGGSIITISSA
                     AGIKAVPGCGHYCASKFGVVGLTNSLAVELGEFGIRVNSVHPYGTDTPMGNDTAMWKI
                     FQDHPNYIHSYSPGALPTDSLADPDLISDIVVWLASDASSLVTAAQIPADKGYLKI"
     gene            complement(4013..4543)
                     /locus_tag="VFC01_03755"
     CDS             complement(4013..4543)
                     /locus_tag="VFC01_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZN78794.1"
                     /translation="MSPEPIEPDTKDWTWVLARPCPECGFDATSLHHTDVAARIRDDA
                     AAWVSRLNEPSVRARPQPTVWSALEYGCHVRDVHRIFNHRVHLMLTEDDPQFPNWDQD
                     ETAIADDYASQDPATVATELFDAAGIVADRYANVPADAWTRRGLRSNGSEFTVASIAI
                     YHLHDIVHHAHDVDHG"
     gene            complement(4540..5217)
                     /locus_tag="VFC01_03760"
     CDS             complement(4540..5217)
                     /locus_tag="VFC01_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN78795.1"
                     /translation="METLPLTPQLSMLRINGWQVYVWRDGDSVTLIDTGAPGSAAEIL
                     SAVAGVDRIVLTHGHVDHSGSAAELHNATGAPVLAGAGDATAIQTGAALPPPVFEDWE
                     VPIYQRVSAGLPETAEPVAVDRELHDGDLVDFGGGAEILAIPGHTEGSIAVYLPHHCV
                     LFTGDTIANVGTVMLGTFNQNRAQTVASFQRLAGLDVETACFGHGQPIVSGAGDRIRD
                     VAATLMP"
     gene            complement(5259..5642)
                     /locus_tag="VFC01_03765"
     CDS             complement(5259..5642)
                     /locus_tag="VFC01_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN78796.1"
                     /translation="MLAFRRAVEARELDVIAELLADDVVLHSPISFKPYHGRENVAKI
                     INLVASILQDFVYQREIGAESDNDHALVFGARVGDLSIQGCDFIHTDTDGLIDELTVM
                     MRPLRAVHAFETQMRARFAESFASS"
     gene            complement(5671..6186)
                     /locus_tag="VFC01_03770"
     CDS             complement(5671..6186)
                     /locus_tag="VFC01_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78797.1"
                     /translation="MSDSTRGALETKSEFDESRARVRRIAVPPTARALSTLADIDYAD
                     AFLVETGTASSPEQCARAVLDGAPLAVRANLQFGWSAIGLRPAIGRARKSILGWEIRA
                     STPEFVLLGRDSLIGMPGELLFKRERDAVLFCTFVQHKTMVARATWAAIESSHLRIVG
                     DLLEQAAHRLV"
     gene            6372..6830
                     /locus_tag="VFC01_03775"
     CDS             6372..6830
                     /locus_tag="VFC01_03775"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN78798.1"
                     /translation="MTKKNLDASIHIAAAPERVWSVLADLTRMPEWSPQCRRMQPLGR
                     VCEGSYTVNWNRQGWKFWPTASKVESFEPNEAIAFRTLTNNSTWLFEIRPAADGTTLT
                     ERRLVPPKGTAWVSKTIVQHVLGGEDAFDDEMLGGMKTTLAKIKAAVEAR"
     gene            complement(6843..8489)
                     /locus_tag="VFC01_03780"
     CDS             complement(6843..8489)
                     /locus_tag="VFC01_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZN78799.1"
                     /translation="MGRPPTAIDAVAERYLDTFASLDPCAATEMGIIGHDDDITDYSP
                     DGVTARVDAARSALRELDGTSPADDVDQVTIAAMCERLGLQIELHDARLDIGDLNVIA
                     SPLQSMRDVFDLMATDTDEDWALIGRRMSKVPDRVAGYVEALRAAVTAGRAPAARQVA
                     RGIQQAGQIQQLFVDMVADAAPENAVLHAELQQRAAAAADSYAALAAALRDEIAPHAR
                     YEDAFGRDAYRLLSREFLGTSVDLDETYEWGLRQLESIVAEQQTIADRLYPGASVSEA
                     LRRLDDEPRYLVHGTEALQAWMQDLSDRAVQSLADTHFDIAAPLRTLECRIAPTHTGG
                     IYYTGPSEDLSRPGQMWWSVPTGVETFRTWQETTTVFHEGVPGHHLQIGRAVVSSDRL
                     NRWRRMGCWVSGHGEGWALYAERLMADLGWLDDAGNRMGMLDAQRFRAARVVIDIGVH
                     CGLTAPDGGVWDAARAWAFLQSHSAMAEENLRFELDRYLGWPGQAPSYAIGQRLWQQL
                     RDETLRRGLSLKEFHSRALDLGGLPLDVLRSALIIDAAAS"
     gene            complement(8482..9369)
                     /locus_tag="VFC01_03785"
     CDS             complement(8482..9369)
                     /locus_tag="VFC01_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HZN78800.1"
                     /translation="MTFNEGMQIDTTNISTGGGRGPGRGIAIGGGLGGLLIVVIALFL
                     GVDPSSVMPQQQEIDAGGVEAPGYDLSHCKTGADANKFVECRVVATTNSVDGVWQQLT
                     PGYTPPHTTLFHGAVQTGCGPADTSVGPFYCPVDQTAYFDTDFFQVLVDQFGSSGGPL
                     AQEYVVAHEYGHHVQNLRGVLGRAQQDPTGAEGAGVRTELQADCYAGVWAHYAAITKQ
                     ESTGVPFLEPLSDKDIADALSAASSVGDDRIQKEATGRINPEQWTHGSSAERQKWFTV
                     GYQTGDPNKCDTFATNNLG"
     gene            9442..10947
                     /locus_tag="VFC01_03790"
     CDS             9442..10947
                     /locus_tag="VFC01_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HZN78801.1"
                     /translation="MAVLLGHSTIAHTGLGDLRGTTEGGVGVWRGVSYAEQPVGERRF
                     LAPAPLQPWTGVRDALEHGPLPPQGKSFVGGGRDDPKVRDEACLTLTVWSPDTSGSLP
                     VMVWIPGGAFVFGAGQFQLYNGSRLAANGNVVVVNVTYRIGVFGGFELSDLGDGFDDN
                     LALRDQLAALRWIRENIASFGGDPDRVTVFGESAGATSVLALLTSPAADGLFRRAIAQ
                     SPALPLIADRELRARRAHDFLKRLGVTAAEVKGLPQRQLRRAAGMLQLKSAASTPTLA
                     YGLTYGVDLLPKHPVDAAREGALARVPLIIGTNSHEASMFAWTKPPMLPTTTASIDAY
                     LNRVAPDAKERVLAAYPDYPRRRALVAFGSDVMFGGPAWAFADAYSAHAPTHMYRFDH
                     FGISLRVLGLGATHGSEIVHIQHSYASFIGRKLHPLGRRLQPPVGRRMQRAWLDFAAK
                     GWESGEIHWSSGHDWPVYDTDDRFTRIIQSARDTVVSDPDAERREAWVGLY"
     gene            10955..12508
                     /locus_tag="VFC01_03795"
     CDS             10955..12508
                     /locus_tag="VFC01_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN78802.1"
                     /translation="MSLVGRVIGLLAVVVLAGCGQGGPAGGTSSATSVAPRPAPPAAS
                     YAKAPCPNPIYTGVPQLDLGPDVECGYLTVPQNRADPNSRKIKLAVVTRKTTSPDPKP
                     DPVVYLAGGPGGTPMVHNFDNWQLDRDLILLGQRGTMKDDPFLACPEVDQFLVKAVGI
                     GAENSAYSQQSAAAVRACRDRIAKDVIDVAAYNTTESVADVADLRTTMGIDQWNIYAL
                     SYGTDLALQVLRDRPDGIRSVVLDSVLPPPANLAESGWDWAAQSYEAIFDACAVDPGC
                     SEAFPDARAEFTRMVNELSASPIRVTVDVGGTPTDVVIDGYKLASGVVVAAAQTPGQL
                     ARIPAMIHKLATGDPTDVGQALAAVTPPNILSYGVMYSVLCREAVARTSPDKVLAVGK
                     QALPDFPDAVLKLPAQVPTLFSDCEQWSVPAAPWWVFDLAHSDVPTLLVSGSFDSATP
                     PPLAEEAAQTLSNSRHYVFPGTGHEVALNTREAAGCLLSVMRSFFDNPVEYNADCVAT
                     LTVPPFQTA"
     gene            complement(12632..13132)
                     /locus_tag="VFC01_03800"
     CDS             complement(12632..13132)
                     /locus_tag="VFC01_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN78803.1"
                     /translation="MAMKPCERVGLDFIDSAPFRFVSTVDLNITPEQLFEVLDDAESW
                     PQWATAITKVTWTSPQPHGIGTTRTVDMRGGIVGDEEFLAWEPYSHMAFRFNEASTGS
                     IAAFAEDYRIVPTASGCHLTWVMAMKPKGVAARLGMTLGRPVMARTFQKFLYNLRDYT
                     DKRFAA"
     gene            complement(13152..14039)
                     /locus_tag="VFC01_03805"
     CDS             complement(13152..14039)
                     /locus_tag="VFC01_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZN78804.1"
                     /translation="MGSRIALVGPGAIGATVAAYLHAAGHSVLVCGHTARESIEVRPD
                     DKEPIVLPGPVHTDPAEVDGPVDVLFIAVKDTQNEQAGAWLARLCHERTVVCALQNGV
                     EQVERVGRFCPSSTVVPAAIWLSSEIQPEGWVRVRNEPRLVLPDSTAASTLAELLRDA
                     GVTVELDPDFSTAAWHKLLVNAVVAFMVLTGRRSGIFRRDDVAALGRRYLAECLAVAR
                     AEGANLSDEVIDEIIGMLAQAPPDVTTSMLTDREAGRPLEWDIRNGVILRKAAAHGIA
                     TPISEVLVPLLAAASDGPG"
     gene            14116..>15725
                     /gene="aspS"
                     /locus_tag="VFC01_03810"
     CDS             14116..>15725
                     /gene="aspS"
                     /locus_tag="VFC01_03810"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZN78805.1"
                     /translation="MLRTHGAGSLRAADAGQTVTLAGWVARRRDHGGVIFIDLRDASG
                     VSQVVFREGDVLAQAHRLRAEYCIAVEGVVEIRPEGNENPEIPTGEIEVNATSLTVLG
                     ESAPLPFQLDEPAGEEARLKYRYLDLRRDGPGAAIRLRSKANAAAREVLARHEFVEIE
                     TPTMTRSTPEGARDFLVPARLQPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDE
                     DFRADRQPEFTQLDMELSFVDTEDVIAISEEILKALWALIGYDVPVPLPRMTYADAMR
                     RFGSDKPDLRFGLELVECTDYFKATEFRVFQAPYVGAVVMPGGASQPRRTLDAWQDFA
                     KQRGHRGLAYVLVNDDGTLGGPVAKNLCDAERDGLAAHVGASPGDCVFFSAGPAKSSR
                     ALLGAVRIEVAKRLDLIDPNAWAFTWVVDPPLFEPADEATAHGDVAVGSGAWTAVHHA
                     FTAPKPDFEDSIESDPGAALADAYDVVCNGNEIGGGSIRIHRRDVQEKVFGVMGLDKA
                     EAEEKFGFLLEAFTFGAPPHGGIAFGWDR"
BASE COUNT         2673 a         5166 c         5136 g         2750 t
ORIGIN      
        1 ggtcctgcct gccagctcgg cgaccagggc gaggtccgcc acgccatcgc cggacacgct
       61 gccgccgccg agatcctcgg gcaccagcag gctcgtccag cctagctcgg cggcacgttg
      121 ccaccagcca gcctcaaagg acgcaccggc cgcatggagt tcacgcagcc gggctagtga
      181 ggcttccttc tccagaaacg actgtgttgt cgaggcgaac agcagccgtt cgggatttgc
      241 gtcttcggtc atgaatcggt tcaggcggga atgacgagtg gcggcacgtc agggttgtgg
      301 acgatcttgt tctccacctt gaacaggttg atcatgttgc cgcccatgac ctttcgctga
      361 ccctcctcat cgagaccctg gaggtcgtcg atcagatggg tcggctcggc cagcccctcg
      421 ggatgcggcc agtcggaacc gaagatcacc cggtcgacgc cgcacaggtc cgccatctgc
      481 gcgaagtcgt cctcccagaa cggtgccacg tagacgccac gcttgaaggc ctcgatcggg
      541 ttctccggga acgcctgcgg catcttcttg taggtgttct cgaactgctt gaacaggtag
      601 ggcacccagt ccgcaccgtt ctcgatcgag aggacgcgca gatcggggtt gcgggtcagc
      661 gcaccatggc agaccaaggc tgccatcgaa tcctcgatgg gccgcttgcc catcgaaacc
      721 atcctgaacg cggtcggctt gaacggcagg aactcgtcgg caggctccca gtcgttgagg
      781 tactcggcgt acccggagtc ggaggcgtgc atcgacaccg ggatgccggc cttcacgcac
      841 gcgtcccaga acgggtcgaa ctcctccgtg ccgaacgacc gtgtaccgcg catcccgggc
      901 accggcgcgg gccggaccag aacggtcttg gcgccgcgct ccagacacca ctcgagttcc
      961 tcgagggcgc ggtcaacgat cggcagcgtg atgaccggcg tggagaagat ccggttctcg
     1021 tagttgaact gccaggtctc gtacatccac tcgttgagcg cgtgaatgac gtcgtggatc
     1081 agctcggggt catccttcat gcgctcctcg accaggctgg ccagggtcgg gaacatcagc
     1141 gagtagtcga gcccaagtcc gtccatgacc tccagtcgcg ctgcgggctc gcggaacgct
     1201 gggatcgcct tcatgggctt gcccatgacc tcgcggaagc tcttgccacc gctgccgtgc
     1261 cggaagtact cctcctgcgc gccggggcgg gcgacgacct cgaaggtggg gttcgggatg
     1321 taatcgctga cctggttgcg caccatgatc ttggtgcggc cgcggacctc gatgtagtcg
     1381 atgacgttct tgcggtggtc cggcaggaac ttcgtcaacg cctctttggg ctcgtagaag
     1441 tggttgtcgg cgtcgaacac cggatagggg agcttgcggg tcatttccgc ctcctggaag
     1501 tctcgtttag ctctggtggt aatgacatta ccacgtctgg accagcgaaa acaccacccc
     1561 cggagaaagg gatttccagc cagatatcgt gcaggtcaga gggcttgatc gcgccggaac
     1621 aggccgctga tgcagaagtc gtagatgtga tcggcgtcga tgggcacgcc gttgagctcc
     1681 gcgcccagaa cgcgcagccg catggcacca agcgcggact gcatgatcag cgcggcggtg
     1741 gcgtcgacaa caagatcagt tcggaaggcg ccctcggtga tgccgcgatt gatgatgtcc
     1801 ttgatcaact cgtgcagtgg tgagagaacg cgcgcatatt cgcggggcag cgtctcggcg
     1861 aggtggtcgt tgtaatcggt gaggccgcgg ttgaccctgt cctgggtgct ggattcggcg
     1921 ggtgtgcaga tccggtcaac cagcacccgc agcgccgcgg cgctttccag cccggtcgta
     1981 tcctcgcgcc acttgcgcgt cgactcggac atgatcttgt cgatcaaggc cagcagcaac
     2041 tcgtccttgg tggaaaagtg ctggtaaaag gaccgcagtg aggtcttgga ccgctccacg
     2101 acttcgagca cggtgaagtc ggtgcgaccg gtttcgccga gaatcgccag cgccgacttc
     2161 atgaaccgcc tttcgcgcgg ttcggtgtcg acgccggcgg agtccggcga ccgtccagtc
     2221 gacttcgcca tggaacacct ccggtcgtgg ttctggtaat gacgttaccg cttttgtaca
     2281 agctcggtta ctgttccgtg ccatgacgac agattcagcg catccatcct cggaggctca
     2341 gtggaccgtc gagggactgc ttgacctgtt cgatgtgcag ccggacggcg agaaccgctt
     2401 caccgcagag actggtccgg caggggagga tcagcggcag gtggtggagg gcacacaggt
     2461 gctcgcccag gtgattgtcg ctgtggccaa acggttcccg gacaagtcgc tgcgctcggt
     2521 ttatgcggtg ttcgcgcggg ctgtgatggt cagcgccggc ccggtcgagc tggagatcga
     2581 cgtggtcagt gagggccggt ccaccgccac ggcgatcgtc gcggccaagc aaaacggcaa
     2641 acgatgcatc acggcgacgg ttctgctcga cgtgcccacc ggcgacgtga ttcgccatca
     2701 tctgccgcgc ccgcaggtcg ccgcgcccgc cgatgcgaac gtctcggaga tgccgatgac
     2761 tggacgccag ctgcgcctcg tcgacgtcgt cgacgtcaac agtcctgacg aggtcggacc
     2821 gccggagctg tatgcatggt tgcattacga cccgatcccg acccgcgacg accttgcaaa
     2881 ggcgttgatc gcgtacttca ctgggcacct tgggatttcg acgacgatgc gcgcgcacga
     2941 gggcatcggc accgcgcagt cgcacttgac cgtgtcgacg gcgccgatga cagtgtcggt
     3001 cagctttcac gagcccgtgc gctgggacgg ctggcttctc tatacgcacg agagcacgca
     3061 agtcggtgcc ggcatgtcgt atgtgcgggg tgcggtgcat accgagaagg gcgagctgat
     3121 cgcgtcgttc gcgcaggacg cgttgatccg gccgctacgc accacggata cggcgatcaa
     3181 gcaccagtcg cgcctctaga tcttgagata gcccttgtcc gcggggatct gggcggcggt
     3241 caccagtgac gatgcgtcgc tggccagcca caccacgatg tcggagatca ggtcggggtc
     3301 ggccaacgaa tcggtgggca acgcgcccgg cgaatagctg tggatgtagt tggggtggtc
     3361 ctggaagatc ttccacatcg ccgtgtcgtt gcccatcggt gtgtcggtcc cgtacgggtg
     3421 caccgagttg acccgaatcc cgaactcgcc aagctcgacc gccagcgaat tggtcagccc
     3481 gacgacgccg aacttgctgg cgcaataatg gccgcatcct ggaacggcct tgatgccggc
     3541 cgcggagctg atcgtgatga tcgagccgcc gttgcccgcc tcgatcatcg ccggcaccac
     3601 cgccttgatg gtgttccaca gcccggtgag gttgatgtcg aggatgtcct gccactgctg
     3661 cgccgaaatc tcccacagcc gaccccaatt gagcacgccg gcgttcgcga cgacgatatc
     3721 gaggcgcccg aattgttcca acgcgtcggc gaccacgcgc tgctgtccgg cggcgtcgcg
     3781 gacatcgacc tcatcggcga ggatcttgcg accctcgcct tccaccaggc tcaccgtctc
     3841 ggcgaggtcc tcgggtgtgg ccggcggata cccgttgtag tcggtgaccg gcgcgcacgc
     3901 gtcgatcgca acgatgtctg cgcccgctcg cgccaaccgg atgcaatgtg aacggccctg
     3961 cccgcgtgcc gctcccgtca catacgcgac cttgcctgca agcggtttgt cttcagccat
     4021 ggtcgacatc gtgcgcgtgg tgaacgatgt cgtgcaagtg atagatcgca attgatgcca
     4081 ctgtgaattc gctgccgttg ctgcgtaaac cacgccgtgt ccacgcgtcg gcgggcacgt
     4141 tggcatagcg gtcggccacg atgccggcgg cgtcgaaaag ttcggtggcg acggtagccg
     4201 gatcctgcga cgcgtagtcg tcggcgatgg cggtctcgtc ctggtcccag ttggggaact
     4261 gcgggtcgtc ctcggtcagc atcaagtgga cgcggtggtt gaagatgcgg tgcacgtcgc
     4321 gaacgtggca gccgtattcg agtgcggacc acacggtcgg ctggggtcgg gcgcgaacgc
     4381 tgggttcgtt gagcctgctc acccaggcgg cggcgtcgtc gcggatacga gcggcgacgt
     4441 cggtgtggtg cagcgatgtt gcgtcgaagc cgcactcggg gcacgggcgc gccaagaccc
     4501 aggtccagtc tttggtgtcg ggctcgatcg gctcgggact cacggcatca gtgtggcagc
     4561 cacgtcgcga atcctgtcac cggcacccga cacgatgggc tggccgtggc cgaagcaggc
     4621 cgtctcgacg tcgaggccgg ccaaccgttg aaacgacgcc acggtctgcg cccgattctg
     4681 gttgaaggtg cccagcatga cggtgccgac attggcgatg gtgtcgccgg tgaacagcac
     4741 gcaatggtgc ggcaaataga ccgcgataga gccctcagtg tgacccggga tcgccaggat
     4801 ttcggcgccg ccaccgaaat cgacgaggtc gccgtcgtgc agctcacgat ccacggcgac
     4861 cggctccgcc gtctcgggca ggccggccga gacccgttgg tagatgggca cttcccaatc
     4921 ctcgaacacc ggcggtggca atgccgcgcc cgtctggatc gcggtcgcgt cgcccgcgcc
     4981 ggcgagcact ggcgctcccg tcgcattgtg cagttctgcg gccgaaccgc tgtggtcgac
     5041 gtggccgtgg gtcaacacga tacggtcgac acccgctacg gcggacagaa tctcggcagc
     5101 cgatcccggg gcgccggtat cgatcagcgt caccgaatct ccgtcgcgcc acacgtacac
     5161 ctgccagccg ttgatccgca gcatggacag ttgaggcgtc agcggaagtg tctccatgac
     5221 tgcacgctaa gaggccgtcg tcggcctggg gaagcggatc agctactggc gaaactctcg
     5281 gcgaaccgtg ccctcatttg tgtttcgaat gcgtgcacag cccgcagcgg acgcatcatc
     5341 accgtcagct cgtcgatcag cccgtcggtg tcggtgtgta tgaaatcgca accctgaatt
     5401 gacaagtcgc cgacgcgcgc accgaacacc agtgcgtgat cattatcaga ctctgcgccg
     5461 atctcacgct gatacacgaa gtcctgcaga atgctggcca ccaggttgat gatctttgcc
     5521 acattttccc ggccgtggta cggcttaaac gaaatggggc tgtggagcac cacatcatcg
     5581 gcgagaagct cagcgatgac gtccaactca cgtgcttcca ccgcacgacg gaatgcgagc
     5641 atgacggtct cctttcctcg ttgcggttcg ttacaccagc cggtgggcgg cctgctcgag
     5701 gaggtcgccc acgatccgga gatgcgacga ctcgatggct gcccatgtcg cgcgagcaac
     5761 catggtcttg tgttggacga aggtgcagaa caggaccgcg tcgcgctcac gcttgaagag
     5821 cagctcgccc ggcatcccga tcaaggaatc gcggccgagc aatacgaatt ctggtgtgct
     5881 cgctcggatc tcccaaccga ggatcgattt tcgcgctcga ccgattgcag gccttagccc
     5941 gatcgccgac cacccgaact gcaggttcgc tctcacagcc agcggcgcac catccaagac
     6001 ggctcgagcg cactgctcgg gggaactcgc tgtacctgtt tcgacaagga acgcatctgc
     6061 gtagtcgatg tcggccaacg tgctgagtgc ccgggcggtc ggcggcactg cgatccggcg
     6121 caccctggcg cgcgactcgt caaactccga cttcgtttca agcgcgcccc gcgttgaatc
     6181 tgacatccca caactcccaa ggtcgcgaac cgttgtcatc cgtacaatac agaactgtat
     6241 ggatgatgtt gttcgttgtc aacggcttga tcgggctgca taaagtcatt gaccgactgg
     6301 tcggtcggtg ataagttaca ggctgcgttg ccgaaggcac gggctaatct cacacctacc
     6361 tggcggaagc aatgaccaaa aagaaccttg acgcgagcat ccacattgcc gctgctccgg
     6421 aacgtgtctg gagcgtgtta gccgacctca cgcgaatgcc ggagtggagc ccgcagtgtc
     6481 gccgcatgca gccgctgggg agggtgtgtg aaggctccta caccgtcaac tggaaccggc
     6541 aaggttggaa gttctggcca acggcgtcga aggtcgaatc tttcgaaccc aacgaagcca
     6601 tcgcatttcg gaccttgacg aacaacagca cgtggctgtt cgagatcagg ccggccgccg
     6661 acggaaccac actgaccgaa cgccggctgg ttcccccgaa aggtacggcc tgggtgtcga
     6721 aaaccattgt ccagcacgtt ctcggaggtg aggacgcgtt cgacgacgaa atgctcggcg
     6781 ggatgaagac caccctcgcg aagatcaagg ctgccgtcga agccaggtaa cgagacgggg
     6841 catcaactgg ctgcggcatc gatgatgagc gccgaccgaa gtacatccag cggcagcccg
     6901 ccgagatcta gtgcgcggct gtggaattcc ttcagcgaca gcccccggcg gagcgtttcg
     6961 tcgcgtagct gctgccagag ccgctgtccg atcgcatacg acggagcctg cccgggccag
     7021 cccagatagc ggtcgagctc gaagcgcagg ttctcctccg ccatcgcact gtgcgactgc
     7081 aggaatgccc acgcccgtgc ggcgtcccag acgccgccgt caggcgcggt gagcccacag
     7141 tgcacaccga tgtcgatcac cacgcgcgcg gcccgaaaac gttgtgcgtc cagcattccc
     7201 atccggttgc cggcatcgtc gagccagccg aggtcggcca tcaaccgctc ggcgtagagc
     7261 gcccaaccct cgccgtgtcc cgacacccag cagcccatcc ggcgccatcg gttcagccgg
     7321 tccgatgaca cgaccgcccg gccgatctgc aggtggtggc ccggcacgcc ctcatgaaag
     7381 accgtggtgg tttcctgcca ggtccgaaac gtctcgacac ccgttggcac cgaccaccac
     7441 atctgtccgg gacggctcaa gtcctcggac ggtccggtgt agtagatgcc accggtgtgg
     7501 gtaggagcga tccggcactc caatgtgcgc agaggcgcgg ctatgtcgaa gtgggtatcg
     7561 gccagcgatt gcaccgcgcg gtccgacagg tcctgcatcc atgcttgcag cgcctcggtg
     7621 ccgtgcacca ggtagcgcgg ctcgtcgtcc agccgccgca gtgcctcgct gaccgaggcg
     7681 ccgggataga gccggtctgc aatcgtttgc tgctcggcga caatgctttc caattgacga
     7741 aggccccact cataggtctc gtcgaggtcg accgaggtgc cgaggaattc ccgtgacagc
     7801 agccggtagg cgtcgcggcc aaatgcgtcc tcgtaccggg cgtgcggcgc gatctcatcg
     7861 cgcagtgcgg cggccagcgc ggcgtaggaa tcggccgctg ccgccgcgcg ttgctgcaat
     7921 tccgcgtgca gcacagcgtt ttccggcgcc gcgtcggcca ccatgtccac aaatagctgt
     7981 tggatttggc ccgcctgctg gatgccacgg gcgacctgcc gcgcggcggg cgcacggccg
     8041 gctgtcaccg cggcccgcag tgcttcgaca tagccggcga cgcggtcggg cacctttgac
     8101 atccgtcggc cgatcagcgc ccagtcctcg tcggtgtcgg tggccatcaa gtcgaacacg
     8161 tcgcgcatcg actgcagcgg tgaagcgatc acgttcagat cgccgatgtc caatcgggcg
     8221 tcgtgcagtt cgatttgcaa accgagtcgt tcgcacatcg ccgcaatcgt cacttggtcg
     8281 acgtcgtcag ccgggctggt tccgtcgagc tcccgcagcg ccgagcgggc ggcgtcgacc
     8341 cgcgccgtca caccgtcggg ggagtagtcg gtgatgtcgt catcgtggcc gatgatgccc
     8401 atttccgttg ccgcgcaagg gtccaacgac gcgaacgtgt cgaggtagcg ttcggcgacg
     8461 gcgtcgatgg ccgtaggtgg cctacccaag gttgttcgtc gcgaaggtgt cgcacttgtt
     8521 ggggtcgccg gtctggtagc cgaccgtgaa ccacttctgc cgctcggccg acgatccgtg
     8581 ggtccattgc tccgggttga tccgcccggt cgcctctttc tggattcggt catcgcctac
     8641 ggatgaggct gccgacagcg catcggcaat gtccttgtcg ctcaacggct caaggaacgg
     8701 cacgccggtg ctctcctgtt tggtgatcgc cgcgtagtgc gcccagaccc ccgcatagca
     8761 gtcggcctga agctcggtgc gcaccccggc gccctctgcg cccgtcgggt cctgctgcgc
     8821 gcggccaaga acgcccctga ggttctgcac atggtgaccg tactcgtggg ccaccacata
     8881 ctcctgcgcc agcgggccac cgctggaacc gaactggtcg acgagcacct ggaagaagtc
     8941 cgtgtcgaaa taggcggtct ggtcgacggg gcaatagaag gggccgacgg aggtgtcggc
     9001 gggtccgcag cccgtctgga ccgcaccgtg aaacagcgtg gtgtgcggag gcgtgtagcc
     9061 cggtgtcagc tgctgccaca cgccgtcgac cgagttcgtc gtcgcgacca cccggcattc
     9121 gacgaacttg ttggcgtccg cgccggtctt gcagtggctc aggtcgtatc ccggggcttc
     9181 gacgccgcct gcgtcgattt cctgttgctg cggcatcacc gagctggggt cgacgccgag
     9241 gaacaacgcg atcacaacga tcagcaatcc gccgagcccg ccaccgatcg cgattccacg
     9301 accgggcccg cggccaccac ccgtcgagat gttggtcgtg tcaatctgca taccctcgtt
     9361 gaaggtcatt gcaccgctcc gtcgctcgcc acaccctgtt cggacccagg cccgtccagc
     9421 tttgcacact acgatttagg cgtggcagtc cttcttggac attcgaccat cgcgcatacg
     9481 gggctgggag atctgcgcgg caccaccgag ggtggtgtcg gtgtctggcg cggcgtgtcg
     9541 tacgccgagc agcccgtcgg cgaacgccgg tttctggcac cggccccgct gcagccgtgg
     9601 accggcgtgc gggatgcgct cgaacacgga cccctcccgc cgcagggcaa gtcgttcgtc
     9661 ggcggcggtc gcgacgaccc gaaggtgcgc gacgaagcct gtttgacgct gacggtgtgg
     9721 tcgcccgaca ccagcggctc gttgccggtg atggtgtgga ttcccggcgg cgcgtttgta
     9781 tttggcgccg gccaattcca gctctacaac gggtcgcgac tggcggccaa cggaaacgtg
     9841 gtcgtggtca acgtcaccta tcggatcgga gtgttcggcg gcttcgaact gtccgacctc
     9901 ggcgacggat tcgacgacaa cctcgcgctg cgcgatcagc tggcggcgtt gcgatggatt
     9961 cgggagaaca tcgcatcgtt cggcggtgat cccgaccggg tgaccgtgtt cggcgagtcg
    10021 gccggcgcga cctcggtgct cgcgctgttg accagtcccg cggccgacgg cctgttccgc
    10081 cgcgcgatcg cgcagagccc cgcgcttccg ctgatcgctg accgcgagtt acgggcgcgg
    10141 cgggcccacg acttcctgaa acggctgggc gtgaccgctg ccgaggtcaa ggggctgccc
    10201 cagcggcagc tgcgccgcgc cgcgggcatg ctgcagctca agagcgccgc aagcaccccg
    10261 acgctggcgt acggcctgac ctacggcgtc gacctgctgc cgaaacaccc ggtcgacgct
    10321 gcccgcgagg gcgccctggc gcgggtgccg ctgatcatcg gcaccaacag ccacgaggca
    10381 tcgatgttcg cctggactaa gccgccgatg ctgcccacga ccaccgcgtc aatcgacgcc
    10441 tacctcaacc gcgtcgcccc cgacgccaag gagcgcgtgc tcgccgcgta cccggactac
    10501 ccacgcagac gcgcactcgt cgcgttcggg tctgacgtca tgttcggggg gccggcctgg
    10561 gcgttcgccg acgcctacag cgcgcacgcg ccgacgcata tgtaccggtt cgaccacttc
    10621 gggatcagcc tgcgggtgct cggcctcggc gccacccacg gcagcgagat cgtgcacatt
    10681 cagcacagct atgcctcgtt catcggccgc aaactgcacc cgttggggcg gcggctgcag
    10741 ccgcccgtcg gtcggcgcat gcagcgcgcc tggctggact tcgcggccaa aggctgggag
    10801 agcggcgaaa tccattggtc cagtgggcac gattggcccg tgtacgacac cgacgatcga
    10861 ttcacccgca tcatccagtc cgcccgcgac acggtggtat cggatccaga cgccgagcgc
    10921 cgcgaggcgt gggtcggcct gtattgaggc cgcaatgagc cttgttggtc gcgtgatcgg
    10981 actgctcgcc gtcgtcgttc tcgcgggatg cggccagggc ggaccggctg gcgggacgtc
    11041 gagcgcgacg agcgtcgcac cgcggcctgc gccgcccgcc gcctcgtacg caaaagcgcc
    11101 gtgccccaac ccgatttaca ccggggtgcc gcagctcgac ctaggtcccg acgtcgaatg
    11161 cggctatctc accgtgcccc agaatcgcgc cgatccgaac agcaggaaga tcaagctcgc
    11221 tgtcgtcacg cgcaagacga catcgccgga cccaaaaccc gatcccgtgg tctatctcgc
    11281 gggcggcccc ggtggcacac cgatggtcca caacttcgac aactggcaac tcgaccgcga
    11341 tctgattctg ctcggccagc gcggcacgat gaaggacgac ccgttcctgg cgtgccccga
    11401 agtggaccaa ttccttgtca aggcagtcgg aatcggcgcc gagaattccg cctattcgca
    11461 acagagcgcg gcggccgtgc gtgcgtgtcg cgaccggatc gcgaaggacg tcatcgacgt
    11521 cgcggcctac aacaccaccg aaagcgtcgc cgacgtcgcc gacctgcgca ccacgatggg
    11581 catcgaccag tggaacatct acgcgctgtc ctacggcacc gacctagcgc tgcaggtgct
    11641 gcgtgaccgt ccagacggca tccgctcggt ggtgctggac tccgtattgc cgccaccggc
    11701 caacctcgcc gaatcaggtt gggactgggc agcgcagagc tacgaggcga tcttcgacgc
    11761 gtgcgcggtc gacccgggat gcagcgaggc ttttcccgac gcgcgcgcgg agttcacgcg
    11821 catggtcaac gagttgtcgg cgagccccat ccgagtcacc gtggacgtgg gcggcacacc
    11881 gaccgacgtc gtcatcgacg ggtacaaact cgccagcggc gtggtggtgg cggccgctca
    11941 gacaccgggc caactggccc gcattccggc gatgattcac aagttggcca ccggcgatcc
    12001 gaccgacgtg ggccaggcgc tcgcggcggt gacgccgccg aacatcctca gctacggcgt
    12061 gatgtacagc gtgctgtgcc gcgaagcggt ggcccgcacc agtcccgaca aggtgctcgc
    12121 tgtggggaag caggcgctac ccgatttccc cgacgccgtc ttgaagctgc ccgcgcaggt
    12181 gccgacgctt ttcagcgact gcgaacagtg gtcggtgccc gcggcgccgt ggtgggtctt
    12241 cgacttggcc cacagcgacg tgccgaccct gctcgtcagc ggcagcttcg actccgcgac
    12301 gccgccgccg ttggccgagg aagcggccca gacattatcc aactcacggc attacgtctt
    12361 cccgggcacc ggccatgaag tcgcgctgaa tacccgtgag gccgcgggct gccttctttc
    12421 tgtgatgcga agtttcttcg acaatccagt cgagtacaac gctgattgtg ttgcgacgct
    12481 tactgttcca ccatttcaga ctgcctagcc gccgaggcag gccgggtgtg tgaggcacag
    12541 cagtgaccta tcggaagtcg ggaggggagg aggatcgcgc catacgaacg gttcgtcaga
    12601 ctccttggac gagtacccat accggatggc gttaagccgc aaatcgcttg tcggtgtagt
    12661 cgcgcaggtt atacaggaac ttctggaacg tccgcgccat taccggccgg ccgagtgtca
    12721 tccccaaccg cgcggcaacg cctttcggct tcatcgccat cacccacgtc aaatggcagc
    12781 cactggccgt cggcactatc cgatagtcct ccgcgaacgc tgcgatactg cccgtcgagg
    12841 cttcattgaa acgaaaagcc atgtgcgagt agggctccca ggccaggaat tcctcgtcgc
    12901 cgacgatgcc gccgcgcatg tccacagttc gtgtcgtgcc gataccgtgc ggctgcgggc
    12961 tggtccatgt caccttggtg atcgctgttg cccattgcgg ccacgactcg gcgtcgtcga
    13021 gcacctcgaa cagctgctcg ggggtgatgt tcagatcgac ggtgctcacg aagcggaacg
    13081 gtgcgctgtc aatgaagtcc aggccgacgc gctcgcaggg tttcatggcc atacacccta
    13141 gaggacgctg tctagcctgg tccgtcgctt gccgcagcga gcagcggcac caacacctcg
    13201 ctgatcgggg tggcaatgcc gtgagccgcc gccttgcgca ggatgacgcc gttgcggatg
    13261 tcccattcca acggccggcc ggcttcgcgg tcggtcagca tcgaggtggt gacatcgggc
    13321 ggggcctgcg ccagcatgcc gatgatctcg tcgatcacct cgtcgctcag gttggcaccc
    13381 tccgcccgcg cgaccgcgag gcattcggcc agataccgac ggcccaacgc ggccacgtcg
    13441 tcgcgacgga agatgccaga gcgcctaccg gtcaacacca tgaaggcgac cacggcgttc
    13501 accagcagct tgtgccacgc ggcggtactg aagtccgggt cgagctcgac cgtgacgccc
    13561 gcgtcgcgca acaactccgc gagcgtcgat gccgccgtgg agtcgggcag caccagccgc
    13621 ggctcgttgc gtacccgcac ccatccctcc ggctggatct ccgacgacag ccagatggcc
    13681 gcgggcacga ccgtcgacga cgggcagaac cgtccgacac gctcgacctg ttcgacgccg
    13741 ttctgcagcg cgcacaccac cgtgcgctcg tggcacagcc gcgccaacca ggctccggcc
    13801 tgctcgttct gggtgtcctt gacggcgatg aacagcacgt cgactggacc gtcgacctcg
    13861 gcgggatcgg tatgcaccgg ccccggcagc acgatcggct ccttgtcgtc gggccgaacc
    13921 tcgatcgact cgcgcgcggt gtgcccgcac accaggacgg aatgaccggc cgcgtgcagg
    13981 tacgcggcga cggtcgcgcc gatagcgcca gggccgacga gtgcgatgcg gctacccatg
    14041 atcgccaagc taccggcgcc gaggccccct ctacacttgg ctgtcgtctc atcaggtcat
    14101 tgacagggag tttttgtgct gcgcacacat ggggccggtt cgttgcgggc cgccgacgcc
    14161 gggcagacgg tgacgctggc cggttgggtg gcgcgtcgcc gtgaccacgg cggcgtcatc
    14221 ttcatcgacc tgcgtgacgc gtccggtgta tcgcaggtgg tgttccgcga aggcgacgtg
    14281 cttgcccagg cgcaccggct gcgcgccgag tactgcatcg ccgtcgaagg tgtcgtcgag
    14341 atccgccccg agggcaatga aaaccccgaa ataccgacgg gcgagatcga ggtcaacgcc
    14401 acgtcgctga cggtgttggg ggagagcgca ccgctgccgt tccagctcga tgagccggca
    14461 ggcgaagagg ccaggctgaa gtaccgctac ctggatctgc gccgggacgg cccaggtgcg
    14521 gcgattcggt tgcgctccaa ggcgaatgcg gccgcccgcg aggtgctcgc gcgtcacgag
    14581 ttcgtcgaaa tcgagacgcc gacgatgacc cggtcgacgc ccgagggggc ccgcgacttc
    14641 ctggtgcccg cgcggctgca gcccggctcg ttctacgcgc tcccgcagag cccgcagctg
    14701 ttcaagcagc tgctgatggt cgccggcatg gagcgctact accaaatcgc ccggtgctac
    14761 cgcgatgagg actttcgcgc agatcggcaa cccgagttca cccagctcga tatggagctc
    14821 agcttcgtcg acaccgagga cgtcatcgcg atctcggagg agatcctcaa ggcgttgtgg
    14881 gccctgatcg gctatgacgt gccggtgccg ctgccgcgga tgacctatgc cgacgcgatg
    14941 cgccgattcg gttcggacaa acccgatctg cggttcggcc tcgaactggt cgagtgcacg
    15001 gactacttca aggccacgga gttccgcgtg ttccaggcgc cctacgtcgg cgccgtggtc
    15061 atgcccgggg gcgcatcgca gccgcggcgc acgctcgatg cctggcagga cttcgccaag
    15121 cagcgcgggc accgcgggct ggcctacgtg ctggtgaacg acgacggcac gctgggcggg
    15181 ccggtggcca agaacctgtg cgacgccgag cgcgatgggc tggctgccca cgtcggcgcg
    15241 tcacccggtg actgtgtctt cttttccgcc ggcccggcca agtcgtcgcg ggcgctgctc
    15301 ggcgcggtcc gcatcgaggt cgccaagcgg ctcgacctga tcgacccgaa cgcatgggcg
    15361 ttcacctggg tggtcgaccc gccgctgttc gaacccgccg acgaggcaac cgcgcacggc
    15421 gacgtcgccg tcggatcggg cgcctggacg gcggtacacc acgcgttcac cgcgcccaag
    15481 ccggacttcg aggacagcat cgaatccgac ccgggtgccg cgctggccga cgcgtacgac
    15541 gtggtgtgca atggcaacga gatcggcggc ggctcgattc gtatccaccg gcgagacgtt
    15601 caggaaaagg tcttcggcgt gatgggcctg gacaaggccg aagccgagga gaagttcgga
    15661 ttcctgttgg aagcgtttac ctttggcgcg ccgccgcatg gcggcatcgc gttcggatgg
    15721 gaccg
//
LOCUS       DASRJA010000084        14337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3311111,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000084 DASRJA010000000
VERSION     DASRJA010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14337
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3311111"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..622)
                     /locus_tag="VFC01_03815"
     CDS             complement(158..622)
                     /locus_tag="VFC01_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZN78806.1"
                     /translation="MDKRNSRVAAAVAAAGVEPNVKILDVDAKTAVAAAAQLGCEVGA
                     IANSLVFDCDGAPLLVMASGAHRVDTDHLARILGATAIRKANPQLVRSSTDQVIGGVA
                     PTGHPSRLRTIVDETLARYPVIWTAAGTADSVMSMTYEELLVVTEGVAISVR"
     gene            641..1534
                     /locus_tag="VFC01_03820"
     CDS             641..1534
                     /locus_tag="VFC01_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN78807.1"
                     /translation="MSSRGWILFLAMSIIWGIPYLMIKVAVEGVSVPVLVLARTAVGA
                     AVLLPLAFSRQTLTIVRNHWKALLGFAFFEIIAAWWLLSDAERHLTSSMTGLLIAASP
                     IIAAVLDRFTGGERLGYKRIIGLSIGIAGVAVLAGPHIGGSTWPIIEVLMVSTCYAIA
                     PLIAARYLADVPALPMTAVCLGFAALVYAAPAAATWPDEMPSTRVLLALGGLAVICTA
                     LAFIVFFALIREVGAARALVFTYINPAVALAAGVIVLHEPLTAWNVAALTLIMVGCVL
                     ATRRHPEHEPDIVTEATVAPR"
     gene            complement(1553..2017)
                     /locus_tag="VFC01_03825"
     CDS             complement(1553..2017)
                     /locus_tag="VFC01_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78808.1"
                     /translation="MLSPEPELIWQGRVHLGDNPGVFADASYSGLVVEFPVTLTKRDA
                     TGSHATTLRLRSENVRTFEPYPGHRIAVTMYEDSGSTVLQKRYIKGAGGDAPVDTEID
                     LDLKGQPPSIFTGVRVQVDTSVPHGLYDDFVVTQLWNKSDNYNVVAALGFNG"
     gene            2430..3296
                     /locus_tag="VFC01_03830"
     CDS             2430..3296
                     /locus_tag="VFC01_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020105297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZN78809.1"
                     /translation="MKPAPFDYHCPGDLAEAIALLQQHGDEAKVLAGGQSLIPLLSLR
                     LSRFDHLVDLRAIEELRGVTTDDGSIRVGAMTTQANLARSEDAAAVPLLAKATSHIGH
                     YQIRNRGTLGGSLAHADPAAEYPAVALALDASFEIVGPSGARRIPAADFFISTWVTAI
                     EPDEVLAAAHFPIWSGNCGWAVAEVARRAGDFAMCGAVCGIQLDGQRVSRTALALFGV
                     ASAPVRAHAAESVVTGATVDDLDLPDIGAAVAATLDPPGDVHASGDQRGRMAKTLVPQ
                     VLAAAIDEARHA"
     gene            3289..3768
                     /locus_tag="VFC01_03835"
     CDS             3289..3768
                     /locus_tag="VFC01_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN78810.1"
                     /translation="MREIALTVTVNGDRLSRTVEARRTLADFLRDDLSLTGTHLGCEH
                     GVCGACTVLLDGDAVRSCLMFAVQADGAEVTTIEGLSERNGELTPIQAAFQAEHGLQC
                     GFCTPGFVVSAHAFLEEHPNPSDEEIRDALSGNLCRCTGYQGIIAAVKRAAENGRSS"
     gene            3765..6098
                     /locus_tag="VFC01_03840"
     CDS             3765..6098
                     /locus_tag="VFC01_03840"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZN78811.1"
                     /translation="MTAGRFMGQAIARSEDPRLLTGRGRFIADVVVPGASDAAFLRSE
                     VARGRITGLDVEAARSAPGVRAVYTAADLNPLVHQTSLSGMPDAPFPPKYCLAQDDVR
                     YVGDPIAIVIAENRYLAEDAVELIELDIDVQDPVIDVDAARDGKGDLVHPEIGTNTPQ
                     VIPLFGDPDLEQAFADAADVVRDTIVMHRYVQVPMECRGIIADWDPYGEALTVWSATQ
                     SVHEVQAFLAGLLGINSNKIRVLQPDVGGGFGQKMMIGRDEWAVVLAATVFGRPVRWI
                     EDRRENLTAASHARDERMDLAMALNGDGTVVAIRGEMIENIGAYQTMGSSGMGAFVAP
                     FLTNAYKIPKVGFASEAVYTNTCGKAPYRGPFLIYAVGIEQLMDTAARQIGMDPLELR
                     RRNVIRAEDLPYTLPSLQCFDAITPAETLEQAASMIDYDGFRDKQAAARDQGRYLGIG
                     ISLCVEPSAIAVGGLGTEAAVVRMDYSGKVTVLLGSSSTGMSVVTTMRQVVAEHLGCA
                     PDDVTVIQGDTAATPYSAGTGGSRSAVLCGNAVAQATIELRSKLTEIAADALEAAVED
                     LEVDDGRVFVRGTATKGMTFAELGQLAYVGHDLLGPGTTPGLEATHRYKAPPFTFSNA
                     CHICTCEVDVDTGLVRILRYVVSEDCGRMINPKVVEGQIFGGVVQGLGGVLYEHMVYD
                     DDGNPLTSTFVDYLLPTAAEVPTIEVGHVESPGPNPLGVKGMGEGGAIASPAAVVNAV
                     ADALAPLGVTVSGSPLGPKQILDMIAAADGHFESTSS"
     gene            complement(6074..7237)
                     /locus_tag="VFC01_03845"
     CDS             complement(6074..7237)
                     /locus_tag="VFC01_03845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN78812.1"
                     /translation="MARLRFGYFIAPFHRAGTNPTLALQRDLEFAEHLDALGYDEIWY
                     GEHHSAGSEIISSPEIFIAAAAERAKRIRFGTGVISLSYHNPLWVADRLVLLDHLTHG
                     RIIGGMGPGSLPSDSAMIGLTPTDTRELLETNLDIVVRLLAGESVTAKTATHELFDAR
                     LQLAPYSDGGIPLAVAAVASPTGARLAGKHGIGLLSIGATLVVEGFDALAYHWGIVEE
                     RAATFGTQVDRRNWTLVGPMHIAETEEQARADVRFGIEPWFRYFQKVAAFPQMTIPGD
                     QIDEMINIINDAGAGVIGTPEQARAQVQRLWDQSGGFGCMLQMGHEWANPAATKRSAE
                     LFAAEVIPHFQGQAQPTVDAAARASEVRDDLAQTQLQAIDHMTKKYQDEVDSK"
     gene            7345..8832
                     /locus_tag="VFC01_03850"
     CDS             7345..8832
                     /locus_tag="VFC01_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZN78813.1"
                     /translation="MTANVAVMGAGPGGLATARWLLSEGFEPTIFEQAPTVGGQWTGL
                     PGLSGVWPTMHTNTSRVLTAFSDLEHETNLVYPSGRDVLDYLSRYAEEFGLMSRIRFG
                     ARVERLNRDDAGWLVDSTGGSERFERVVVATGRFQSPTIPSVLGLDTFAGAAGVITTY
                     EYRDPAPYRGKRVLVAGGAISALEIAADIAHFGAARVVVTQRRQRYVLPKFAAGVPSD
                     YRMFTRYGTLADERLPVAEVDRQLKEIVVEAGGSPEQYGAPAPAPSFFAAGVTLSQHY
                     LPLVAEGRITVRPWIESVEGAAVSFLDGTTEEFDGIIFGTGFAMQLPFLSEEIRAILD
                     LDTVHLDADRYTFHPDLPGLAFVGMWDQGGGYFVPLELQARWIAYSWSGAVAADEAEQ
                     RAAVNAYRARRGMSQKTRMNLVALTFARAAGVEPRLENWPGLRRALLFGPLAPSCFRL
                     EGSDALADAPARFARDAAAFGAITSTELTQREQMYWALVGPRHPW"
     gene            8966..10510
                     /locus_tag="VFC01_03855"
     CDS             8966..10510
                     /locus_tag="VFC01_03855"
                     /inference="COORDINATES: protein motif:HMM:NF019842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HZN78814.1"
                     /translation="MAKHRQPKNRRISSNGQAVTKRRPSPSLVLAGSTAVALSTVLVF
                     GHATNNTADSPQVQLAAATIGIGGRGDPNAANIPNKLQGQVVPFGVPPVNYIPVVYPA
                     GFDIDRSVAAGVPVLDQTIKSNSAQFLMVVGYSEGALVAENVRRDLDPGDPGAPPDNS
                     TNTNNPGLLWVMIASPNIPNGGLAGRFPTGFGIPFIVTSNGPAEPSPYDTQYVTNEYD
                     PYADFPAYFNPLSLVNSLFAVVYVHPDQYYDSIDYDPLTGATNDPNVLVHPPVPNSAG
                     GTDTYVFVLANELPLFAPVRQIAGILQLTPLTEPLLDSVEPLVRLVVDMGYTDRQNLN
                     PQQHVQFSLITPPERILETIAGVPGAIGQGVTNLVTGLPAPVAPTNSPAINAKSLAQE
                     PSESLVNDEPTIAAATDPAPPQTPSGSGTSSPTETPAATTLSDPRPTLGKVTEDGNKA
                     TPNTSSPTTTPKKNVFTELADAVNGFFSPKTKPAPSPPSSDPTGTTPSGSTTQDQSEG
                     PTSNAA"
     gene            complement(10514..12892)
                     /locus_tag="VFC01_03860"
     CDS             complement(10514..12892)
                     /locus_tag="VFC01_03860"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN78815.1"
                     /translation="MTAALAGLRVVELAGDIAGPYCAKLLVDLGAEVTKVEPPSGDPM
                     RGWGPFPGGIPDPNRSGLFEYLNAGKRGATLDLAADSDASAVRDLIARADVLVEALPP
                     GTLDRCGLDARALEAVSPGLVVVRISDFGQHGPLRDRVASPLTLQAAAGWINRRDPER
                     PPVQAGGRISEYVAGGYAALGALTALRTLSANVSHLVEVDVSVLESLLSTLPYPMLMA
                     EKMRNLGLPANTRSAPMMGIVRAADGWLGINCLTGQHWLDVCAMLDLPEYGEHQIAIM
                     LGGPERAEFYGHAQPWLSERTVAEIVDLGQAMRIPVSPVHDGGTVAECPQYRDRGFFV
                     DGGGDGWSFRRPGSPFRLSRTPVLPVRTAPRLGDVATETDSARWPGAGANPVSPFADV
                     KVLDLSTFWAGAYLTCYLGAFGAEIVKVESIQRPDGHRYSGAWAYEGDRWYERSAMWQ
                     GTNLNKRDITLDLTSDQGRDLVRRLVRDADVVVENFSPRVIEQFGLDYESLVALKSDV
                     ILVRMPGFGLEGPWRDYVGWALNFEQTSGMAAVTGYGDGPPCNLQGPADPIVGVHGGV
                     ALLAALEHRRRTGEGQLIEVAQIEVAACVAAEPVIEYSMNAVVPPRDGNRRRGHAQGV
                     YPTVDDDAWVALCVRDDADWAHMVEAMGRTDLLSDPRFASTEQRHLTHDEFDTVVADW
                     TRTQAAGHIVKILAAQHIPAELVLTADRMYDIPQLDARHYYQDVEHPITGPHRYPGWP
                     FRMTPGPRSHHRSAPPTLGQHNDEILRGLGLTDDQLADLRARHVIGEAALNA"
     gene            13084..14304
                     /locus_tag="VFC01_03865"
     CDS             13084..14304
                     /locus_tag="VFC01_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN78816.1"
                     /translation="MICLISTPSREAALRTTFPLHSPDFYAGDPYPAYKELRASAPVC
                     WNDVTKFWALLRYDDIRYVSTNPDKFSSTAGITIPDPVMPNPVQEGALIFTDPPRHRQ
                     LRKLINTGFTRRQVAILEPKVREIVHGVFDDVRAGSTHEFAEELAAPLPTRMIAELLG
                     APPDDWEKFRRWSDACTGNADPEIEMDSLVAVGELFEYFQQLIAVRRTEPRNDMLSVL
                     TTAEVDGAKLTDEDLLNFAFLLLVAGNETTRNLIALGTLALIEHPAEAAKLVEDPGLI
                     PAAVEEMLRWTSPVTHMARAATADIEIRGQQIRRGDMVVMLYGSANRDEEVFGSDAEE
                     FKVTRHPNPHIAFGCGEHSCVGAQLARLEARVFFEVLLGRFPELELVGDVDRMRATMV
                     PGVKRMPVRLGTAS"
BASE COUNT         2547 a         4736 c         4551 g         2503 t
ORIGIN      
        1 cactcaccgc cgatcccacc cgcaactacc agcccagcgc cccaaatacc cggacttacc
       61 gcggccgcga gcccaaacca ccaccatgag tgtcagcgat gtcccgagac ataactgtca
      121 gcgatgtccc gagacaccac agtacgctga gcgtctgtta gcgcaccgaa atcgctacac
      181 cttcggtgac caccaacagc tcttcgtacg tcatcgacat cacggaatcc gcggtgccag
      241 cggcggtcca gatgaccgga taccgggcca acgtctcgtc gacaattgtg cgcagccggc
      301 tcgggtgccc agtgggtgcg acgccgccga tgacctgatc ggtcgagctg cgcaccagtt
      361 gcggattcgc cttgcggatc gcggtggcgc cgaggatgcg cgccaggtga tcggtatcga
      421 cgcggtgagc gccgctagcc atcaccagca gcggtgcacc atcgcagtcg aacaccaggc
      481 tgttcgcaat cgcgccgacc tcacagccga gttgggccgc cgccgcgacc gcggtcttcg
      541 catcgacgtc caggatcttg acgttgggct cgacgcctgc ggcggccacc gcggccgcca
      601 cccgggaatt tcgcttgtcc acgtttcgta gggtatgtat gtgagctcgc gtggatggat
      661 cctgttcctc gcgatgagca tcatctgggg catcccatac ctgatgatca aggttgccgt
      721 tgaaggtgtg tcggttcccg tgctggtact ggcccgcacc gcggtcgggg cggcggtgct
      781 gctgccgctg gccttctccc ggcagaccct gaccatcgtc cgcaaccact ggaaggcgct
      841 gctggggttc gcgttcttcg agatcatcgc cgcgtggtgg ctgctctcag acgccgagcg
      901 ccatctgacc agttcgatga ccggtctgct catcgctgca tcacccatca tcgccgcagt
      961 cctcgaccgc ttcaccggcg gtgagcggtt gggttacaaa cgcatcatcg gcctctccat
     1021 cggcatcgcc ggggtggccg tgctggccgg tccgcatatc ggcggcagca cctggccgat
     1081 catcgaggtg ttgatggtgt cgacgtgcta cgcgatcgcg ccgctgatcg ccgcgcgcta
     1141 cctggccgac gtgccagctc tgccgatgac cgcggtatgc cttggcttcg ccgcgctggt
     1201 gtacgccgct cccgcagcgg ctacctggcc cgacgaaatg ccgtccaccc gagtgctttt
     1261 ggcgctgggc ggactcgcgg tcatctgcac cgccttggcg tttatcgtgt tcttcgcgct
     1321 catccgcgaa gtcggtgcgg cgcgtgcgct cgtgttcacc tacatcaatc cggcggtcgc
     1381 cttggcagcc ggggtgatcg tattgcacga gccgctgacg gcgtggaacg tcgcggcgtt
     1441 gaccctcatc atggtgggtt gcgtcttggc gacccggcgg cacccggaac acgaacccga
     1501 catcgtgacg gaggcgacgg ttgcgccgcg gtgacgatgg gctgctgcgc cttcagccgt
     1561 tgaagcccag cgcggcaaca acgttgtagt tgtccgactt gttccacagt tgggtcacaa
     1621 cgaaatcgtc gtacaaaccg tgcggaacgc tggtatccac ttgcacgcgg acaccagtga
     1681 atatggacgg cggctgccct ttcagatcca ggtcgatctc cgtatcaaca ggcgcgtcgc
     1741 cgccggcacc cttgatgtag cgcttctgga gaacggtgct gcccgaatct tcgtacatcg
     1801 tgaccgctat ccggtggccg ggataaggct cgaatgtgcg gacattctcg ctgcgcagcc
     1861 ggagcgtcgt cgcgtgagaa ccggtcgcgt ccctcttggt cagtgtgacg ggaaactcca
     1921 cgacaagacc ggaataagac gcatccgcga agacgcctgg gttgtcaccg agatgtacgc
     1981 ggccttgcca gatcagttcc ggttccgggc tcagcatgga atacccccac tcaatggacg
     2041 ttgccgctga acttaccgat acctcaccac tgttgccgga atttccggaa aagcgttgcg
     2101 tagctatgtg ctgacccacc gggagcgctg gattacgtca agttcgcgga ttcaatcgaa
     2161 tttgctgacg cacgaccgtc acttcgagcc gcggtgagcg cggcgtacgg cgtcaacacc
     2221 gtcgagccgt gggtcttgca gccctccaga ccccggtgcg cttgtgctag caggtagagc
     2281 gcctgctcat tccgcaaacc tgacccgtgc ggatgtgcga tgctgggctc ggtcgtgcac
     2341 tgaagcgggg gccgggggca aatgcagacc ttcgtccgtg accacacgga ttgtccgagc
     2401 cagcatgtgg cgtgccaaag gccgcgccag tgaagcctgc accattcgac taccactgcc
     2461 ccggagatct cgccgaggcg atcgcgctgc tgcaacagca tggcgacgag gcaaaggtgc
     2521 tcgctggcgg gcagagcctg atccctcttc tatcgctgcg tctttcacgg ttcgaccacc
     2581 ttgtcgacct gcgggcgatc gaggaactcc gcggcgttac gaccgatgac ggctcgattc
     2641 gagtgggagc catgaccact caagccaacc tggcccgaag tgaagacgcc gctgccgtgc
     2701 ccctgctcgc caaggcgacg tcgcacatcg gccactatca gatccgcaat cgcggcacgc
     2761 tgggcggctc tcttgcgcat gccgaccccg ccgcggaata cccggcggtg gcgctagcgc
     2821 tcgacgcgtc gttcgagatc gtggggccaa gcggcgcccg gcgcattccc gcggctgact
     2881 tcttcatatc gacctgggtg accgccatcg agccggacga ggtcttggcg gcagcccact
     2941 ttcccatctg gtccggcaac tgcggctggg ccgtcgctga agtggctcgt cgcgcgggcg
     3001 acttcgcgat gtgcggcgcc gtgtgcggga tccaactcga cggccaacga gtgagccgaa
     3061 cggcattggc tctgttcggc gtcgcaagtg caccggtgcg ggcacatgcc gccgaatcgg
     3121 tagtcacggg ggcgacggtg gacgatctcg atttgccgga tatcggggca gcggtcgcgg
     3181 ccaccttgga tccgcccggt gatgttcatg cgtccggtga ccaacgcgga agaatggcca
     3241 aaacccttgt accacaggtg cttgcggccg caatcgatga ggcgagacat gcgtgagata
     3301 gcactgactg tgacggtcaa tggggatcgg ctttcccgca ctgtggaggc gcggcgcacg
     3361 ctcgcggatt tcctgcgtga cgatctgagc ctgaccggca cgcacctggg ctgcgagcac
     3421 ggcgtctgcg gggcgtgcac ggtgttgctc gatggggacg cggtgcgctc ctgcctgatg
     3481 ttcgccgtac aggccgacgg tgccgaagtg acgacgatcg aggggctctc agagcgcaac
     3541 ggcgaactga ctccgatcca agccgccttc caggccgagc acggccttca atgtggtttt
     3601 tgcacaccgg gtttcgtggt ctcggcgcac gccttcctcg aagagcaccc gaacccgtcc
     3661 gacgaggaga tccgcgatgc gctgtcggga aacctctgcc ggtgcaccgg atatcagggc
     3721 atcattgccg cggtgaaacg cgcagcggag aacgggaggt catcatgacc gccggtcgct
     3781 tcatggggca ggcgatcgcc cgctcggaag acccgcgcct actgactgga cgcgggcgct
     3841 tcatcgccga cgtcgtggta cccggcgcat ccgacgcagc gttcctgcgc agcgaggtgg
     3901 cccgtgggcg catcaccggc ctcgacgtcg aggcggcccg gtcagcaccc ggcgtacgag
     3961 ctgtgtacac cgccgccgac ctcaacccgc tggtgcacca gacatctctg tccggcatgc
     4021 ccgatgcgcc atttccgccc aagtactgtc tcgcccaaga cgacgtccgc tatgtcggcg
     4081 acccgatcgc catcgtgatc gccgagaatc gctacctcgc cgaggacgcc gtcgagctga
     4141 ttgagttgga catcgatgtc caggaccccg ttatcgacgt cgacgctgcc cgcgacggca
     4201 aaggcgacct ggtgcacccc gagatcggca ccaacacacc tcaggtgatt ccgctgttcg
     4261 gcgaccctga cctcgagcag gccttcgcgg acgccgcaga cgtagtgcga gacaccatcg
     4321 tgatgcaccg ctacgtgcag gtgccgatgg aatgccgcgg catcattgcc gactgggatc
     4381 cctacgggga ggcgctcacc gtgtggagcg caacccaaag cgttcacgag gtgcaggcgt
     4441 tcctcgccgg gctgctcggc atcaacagca acaaaatccg agtgctgcag ccggacgtcg
     4501 gtggggggtt cggccagaag atgatgatcg gacgcgacga gtgggcagtg gtgctggccg
     4561 ccaccgtgtt cgggcgtccc gtgcgctgga tcgaggaccg acgcgagaac ctgacggcgg
     4621 cctcccacgc ccgtgacgag aggatggacc tcgcgatggc gctgaacggc gacgggactg
     4681 tcgtcgccat ccgaggcgaa atgatcgaga acatcggcgc ctaccagaca atgggatctt
     4741 ctggcatggg cgcctttgtg gcaccgtttc tgacgaacgc ctacaagatt cccaaggtcg
     4801 gatttgcgag tgaggcggtg tacacgaaca cctgcggcaa ggccccttac cgtggcccgt
     4861 tcctgatcta cgccgtgggc atcgaacagc tgatggacac cgcggcccgc caaatcggaa
     4921 tggacccgct tgagctgcgc cgccgcaacg tcatccgcgc cgaggacctg ccgtacacgt
     4981 tgcccagcct gcagtgcttc gatgcgatca ctcccgccga gacgcttgag caggccgcgt
     5041 cgatgatcga ttacgacggc ttccgggaca agcaggcagc cgctcgggat caaggacgct
     5101 acctcggcat cggtatcagc ctgtgcgtgg aaccgtcggc gatcgcggtc ggcggactgg
     5161 gcaccgaggc cgcggtggtc cgaatggatt acagcggcaa ggtcacggtg ttgctcggta
     5221 gctccagcac cggcatgagt gtcgtgacga caatgagaca ggtggtcgcc gaacaccttg
     5281 gctgtgcacc ggacgacgtc accgtcatcc aaggcgacac cgccgccacc ccgtacagtg
     5341 ccggcacggg gggcagccgc agcgcggtgc tatgcggcaa cgcggtcgcc caggcgacca
     5401 tcgagttgcg gtcgaagctg acagagatcg ccgcggatgc actcgaagcg gctgtcgagg
     5461 atctcgaggt cgacgacggt cgcgtcttcg ttcgcggcac cgcaaccaag ggaatgactt
     5521 tcgccgaact gggccaattg gcctacgtag gccatgacct tctcggaccg ggaacgacgc
     5581 caggactcga ggcgactcac cgctacaagg cgccgccgtt cacgttctcc aacgcgtgcc
     5641 acatctgtac gtgtgaggtc gacgtcgaca cgggtctggt gaggatcctc cggtacgtcg
     5701 tcagcgagga ctgcggacga atgatcaacc cgaaggtggt ggagggccag atcttcggcg
     5761 gggtggtcca gggcctcggc ggtgtgctct acgaacacat ggtctacgac gacgacggta
     5821 atccgctcac cagcaccttc gtcgactatc tactgccgac cgcggccgaa gtgcccacca
     5881 ttgaggtcgg ccatgtcgag tcgcctggcc caaacccgtt gggcgtcaag ggaatgggag
     5941 aaggtggtgc gattgcgtcg cctgccgcgg tggtcaacgc cgtcgccgac gcgctcgcac
     6001 cgctcggcgt caccgtctct gggtcgccgc tcggcccgaa gcagatcctc gacatgatcg
     6061 ccgcggccga cggtcacttc gagtcgactt cgtcctgata tttcttcgtc atgtggtcga
     6121 tcgcctgaag ctgcgtctgg gcaaggtcgt cacgcacctc gctcgcacgc gccgccgcat
     6181 ccaccgtcgg ttgtgcctgc ccctggaaat gcggtatcac ctcggcggcg aacaattcgg
     6241 cggaccgctt cgtcgcagcc gggttcgccc attcgtggcc catctgcagc atgcagccga
     6301 acccgccgga ctgatcccaa agccgctgca cctgcgcccg tgcttgctcg ggcgtgccga
     6361 tcactccggc tcccgcgtcg ttgatgatgt tgatcatttc gtcgatctga tcccccggaa
     6421 tcgtcatctg cgggaacgcc gcgaccttct ggaagtaccg gaaccacggc tcgatgccga
     6481 acctgacgtc ggcgcgcgcc tgctcttctg tctcggcgat gtgcatcggg ccaaccaggg
     6541 tccagttgcg gcgatcgacc tgcgtgccga atgtcgccgc ccgctcttcg acgatgcccc
     6601 agtgatacgc gagcgcatcg aagccttcga ccaccagtgt ggcgccaatc gaaagcagac
     6661 caatgccgtg cttgcccgcc agccgcgcac ccgtcggaga ggcgaccgcc gccaccgcca
     6721 gcggaatgcc gccgtcggaa tacggcgcga gttgcaaacg cgcatcgaac aattcgtgcg
     6781 tggcggtttt ggccgtcacg ctttcgcctg ccaacagccg cacgacgatg tcaaggttgg
     6841 tttcgagaag ctcgcgggta tcggtcgggg tcagaccgat catcgcggaa tcgcttggca
     6901 gtgaacccgg ccccatcccg ccgatgatgc ggccatgggt gaggtgatcg agcagcacca
     6961 agcgatcggc tacccacagc gggttgtgat acgagagcga aatgaccccg gtgccgaagc
     7021 gaatgcgctt cgcccgctcg gcggccgccg cgatgaagat ctcgggtgag ctgatgatct
     7081 cgctgcccgc ggagtggtgc tcgccgtacc agatctcgtc gtagccgagc gcatcgaggt
     7141 gctccgcgaa ctcgaggtcc cgctgcaatg ccaatgtcgg gttggtgccc gcgcggtgaa
     7201 acggtgcgat gaagtatccg aacctcagcc tggccatcgg gcgcctcctc gcgcaagcaa
     7261 cgttcgcggt ctagatcaga ccactcttgc atggcgtcat cgtttgatga cgcgtctgga
     7321 ggacaaacat gcgacagttc ccgaatgacg gcaaacgtcg ccgtgatggg tgcgggaccc
     7381 ggcgggctgg ccaccgcgcg gtggctgttg tcggagggat tcgagccgac catctttgaa
     7441 caggcgccga cggttggcgg ccagtggaca ggactccctg gcctcagtgg ggtatggccg
     7501 acgatgcaca ccaacaccag tcgggtcctg acggccttca gcgacctgga gcatgagacc
     7561 aacctcgtgt atccgtcggg ccgcgacgtc ctcgactacc tgagccgcta cgccgaggag
     7621 ttcgggctga tgtcgcgcat ccggttcggc gcgcgggtcg agcgcctcaa ccgagatgac
     7681 gcgggttggc tcgtggactc caccggcggc tccgagcgct tcgaacgagt ggtggtggcg
     7741 acaggtaggt tccaatcccc caccattcca agcgttctcg ggctggacac gttcgcaggc
     7801 gcggcgggtg tgatcaccac atacgagtat cgggaccccg cgccttatcg cgggaagcgt
     7861 gtacttgtcg cgggcggagc gatcagtgcc ctggagatcg ccgccgacat cgcccacttc
     7921 ggcgcggccc gcgtcgtcgt cacccagcgc cggcagcggt acgtgctgcc gaagttcgcg
     7981 gcgggcgtgc cttccgacta tcgaatgttc acccggtacg gaacgctcgc ggacgagcgc
     8041 ctccccgtag cggaggtcga ccggcaactg aaggagatcg ttgtcgaggc gggcggaagc
     8101 cccgagcagt atggcgcgcc ggcgcccgct ccttcattct tcgccgcggg tgtcaccctc
     8161 agccagcact acttaccgct cgtggccgag ggccggatca ccgtccgacc gtggatcgag
     8221 tccgtcgagg gcgcggccgt gtccttcctc gacgggacca cagaggagtt cgacggaatt
     8281 atcttcggca ccggattcgc aatgcagttg ccattcctga gcgaggagat ccgcgcgatt
     8341 ctcgacctcg acacggtgca cctggacgcc gatcgctaca cctttcatcc cgacttgccc
     8401 gggctggcct tcgtcggcat gtgggaccag gggggcggat atttcgtgcc actggagttg
     8461 caagcgaggt ggatcgccta ctcctggagc ggcgcggtgg cggctgatga ggccgaacag
     8521 cgggcggctg tcaatgccta ccgcgcgcgg cgcggtatgt cccagaagac gcggatgaac
     8581 ttggtcgccc tgacgttcgc cagggccgcg ggtgtcgagc cccggctaga gaactggccg
     8641 gggctgcgcc gggctctgct gtttggtcct ctcgcgccga gttgctttcg gctcgagggg
     8701 tcggacgccc ttgccgacgc cccggcccga ttcgctcgcg atgccgctgc gttcggtgcg
     8761 atcacatcga ccgagctgac gcagcgcgaa cagatgtact gggcgctggt ggggccgcgc
     8821 cacccatggt gagccaatgg ccggtaagcg ctccagctaa gatccgtctg tcataggatt
     8881 cgcaccgatt caagcgccaa tctccgcgcc gtgcgataac gttcgacgca cgccgggatt
     8941 cgcggcgatc gtaaaggagc aattcgtggc aaagcaccgc caaccgaaga atcgtcgaat
     9001 ttcgtccaat ggacaggcgg tcacaaagag acggccgtca ccgtcgctgg ttctcgcggg
     9061 ctccaccgct gtcgccctgt cgaccgtcct cgtcttcggt catgcgacca acaacacggc
     9121 ggacagccca caggtgcagc tcgcggccgc caccataggc atcggcggcc gcggcgaccc
     9181 gaacgcggcc aacatcccga acaagttgca gggccaggtc gttcctttcg gtgtcccccc
     9241 tgtcaactac attcccgtcg tgtacccggc cggattcgac atcgacagga gcgtcgccgc
     9301 gggtgttccg gtgctggacc agacgatcaa gtcgaattcc gctcagttcc tgatggtggt
     9361 cggctattcg gaaggagcgc tcgtcgccga gaacgtacgg cgcgaccttg accccggcga
     9421 tcccggtgcg ccgcccgaca actccaccaa cacgaacaat ccgggattgc tctgggtcat
     9481 gatcgcctcg ccgaatatcc cgaacggcgg cctcgccggg cgtttcccaa ccggattcgg
     9541 aatccccttc atcgtcacgt cgaacggtcc cgcggagccg tcgccctacg acacgcagta
     9601 tgtgacgaat gagtacgacc cgtacgccga tttcccggcc tacttcaatc cgctctcgct
     9661 ggtgaactcc ctttttgccg tcgtatatgt ccacccggat cagtactacg acagcatcga
     9721 ctacgacccc ctcaccggcg caacgaacga tcccaatgtc ttggtgcacc ccccggtgcc
     9781 gaacagcgct ggcggcacgg acacctacgt gttcgttcta gccaacgagc tgccgctgtt
     9841 tgcgcccgtg cggcagatcg cgggcatcct gcaattgacg ccactcaccg aaccgttgct
     9901 ggacagcgtc gagccgctgg tgcggttggt cgttgacatg ggttacaccg atcgccaaaa
     9961 cctgaatcct caacagcacg ttcagttttc gttgatcacg cccccagaac gaatcctcga
    10021 gacgatagcc ggggttccgg gcgcgatcgg gcaaggggtg accaacttgg tcactggact
    10081 acccgcgccg gtcgcgccga cgaactcgcc cgcgatcaac gcgaagtcgc tggcgcagga
    10141 gccctcggaa tcgctagtga acgacgagcc gacgatcgcc gcagccaccg acccggcacc
    10201 gccgcagacg ccgtccggct cgggcacctc ctccccgacc gagacaccgg cggcgaccac
    10261 gctgtcggac ccacggccaa cgcttggcaa ggtcaccgag gacggcaaca aagccacgcc
    10321 gaacacgtcc agcccgacca cgacaccgaa gaagaacgtg ttcacggagc tggccgacgc
    10381 ggtcaacggg ttcttcagcc ccaagacgaa gccggcgcca tccccaccga gctcagatcc
    10441 caccggcacc acgccgagcg gctcgaccac gcaagaccag tcggaaggtc cgacgagcaa
    10501 cgccgcctga cagctatgcg ttgagtgcgg cttcgccgat gacatgtcgc gcgcgaaggt
    10561 cggccagctg gtcatcggtg aggccaagac cccgcaggat ctcgtcgttg tgctgcccaa
    10621 gggtcggcgg cgcggaccgg tggtggctgc gggggccggg tgtcatcctg aacggccagc
    10681 cggggtagcg gtgcggcccg gtgatcgggt gctcgacatc ttggtagtag tgccgcgcgt
    10741 cgagttgagg gatgtcatac atgcggtcgg ctgtcagcac cagttctgcc ggaatatgct
    10801 gtgcggcaag gatcttgaca atatggcccg ccgcttgggt gcgggtccag tcggcgacca
    10861 ccgtgtcgaa ctcgtcgtgt gtgagatgcc gttgttccgt cgacgcgaat cgcggatctg
    10921 agagcaggtc cgtacgcccc attgcctcga ccatgtgcgc ccagtcggcg tcgtcgcgca
    10981 cgcacagcgc gacccatgca tcgtcgtcca cggttggata gacaccttgt gcatgaccgc
    11041 ggcggcggtt cccgtcgcgc ggcggcacaa cggcgttcat cgagtactcg atcacgggct
    11101 cggcggcgac gcaggccgcg acctcaatct gagcgacctc gatgagttgc ccttcgccag
    11161 tgcggcgccg atgctcgagc gcggcgagaa gcgcaacgcc gccgtgcacg ccgacgatgg
    11221 gatccgcagg tccctgcaga ttgcacggcg gcccgtcgcc gtacccggtc acggccgcca
    11281 tgcccgacgt ctgctcgaaa ttgagtgccc agccgacata gtcacgccaa ggcccctcca
    11341 ggccaaaccc gggcatccga accaagatca cgtcggactt cagtgccacc agggattcat
    11401 agtccagccc gaattgctcg atgacgcgag gcgagaaatt ctccacgacg acatcggcat
    11461 cgcggacaag tctgcggacc aggtcacgcc cctgatcgga cgtgaggtcc agtgtgatgt
    11521 cccgcttgtt gaggttggtg ccctgccaca ttgcgctgcg ctcgtaccac cggtccccct
    11581 cgtaagccca cgcacccgaa tagcgatgtc cgtcgggtcg ttgtatcgat tcgactttga
    11641 cgatctcggc accgaacgcg ccgagatagc aggtcaaata ggcacccgcc cagaacgtgg
    11701 agaggtcaag gactttgacg tcggcgaacg gcgagaccgg attggcgccg gcgccaggcc
    11761 agcgagccga atccgtctct gtcgcaacgt caccaagtcg gggcgcggtt cgcacaggca
    11821 atacgggcgt ccgtgacagg cggaatgggc tgcccggccg ccggaacgac cagccgtcac
    11881 cgcccccgtc cacaaaaaac ccgcgatccc ggtactgggg gcactctgcg acggttccgc
    11941 cgtcgtggac gggcgaaacc ggaatccgca tcgcctggcc caagtccaca atctcggcca
    12001 cggttcgctc tgacagccat ggctgggcgt ggccgtagaa ctcggcgcgc tcgggacctc
    12061 ccaacatgat cgcgatctgg tgctcgccgt attcgggcag atcgagcatt gcgcacacgt
    12121 ccaaccagtg ctgacccgtc aggcagttga tcccgagcca gccgtccgcg gcgcggacga
    12181 tgcccatcat cggggccgac ctggtgttgg ccggcaagcc gaggttgcgc atcttttcgg
    12241 ccatcagcat cgggtacggc agcgtcgaca gcagcgattc aagcaccgaa acgtccacct
    12301 cgacgaggtg acttacgttg gcggacaggg ttctcagcgc agtgagcgca ccgagcgcgg
    12361 cgtagccacc cgccacatac tccgagatcc ttccgcctgc ctgcaccggc ggtcgctccg
    12421 ggtctctcct gttgatccat cccgctgccg cctgcagggt cagcggcgag gcgacgcggt
    12481 cacgcagcgg cccgtgctga ccgaagtccg agatgcgaac aacgacgagg cctggactga
    12541 ccgcttctaa tgcccgcgcg tctagcccgc agcgatcgag cgttccgggc ggcaaggcct
    12601 cgacgagcac atcagcgcgg gcaatcaagt cgcggacggc cgaagcgtcg ctatccgccg
    12661 cgagatcgag tgtggctccg cgctttccgg cgttgaggta ctcgaacaga ccggaccgat
    12721 tgggatccgg tatcccgccg ggaaatggtc cccagccgcg catgggatct ccggaaggtg
    12781 gttcgacttt ggtgacctcg gcaccgaggt cgacgagcag cttggcgcag tacgggcccg
    12841 cgatgtcccc cgcgagctcg acgacgcgca atcccgcaag tgctgcggtc acgccgggat
    12901 cccgaccgtt ttcggctcca tgcgctttcc tcaccgaagt cagtgaacca acacatggtg
    12961 tacacctgtc ggactcgcca cgtcaatcac gtctacctaa ctcgattgat aaccaacgga
    13021 atcactgagt attgacagtg ccatgcccat cgagtaaaca cagtctctat cagacagatt
    13081 gatatgatct gtctgatttc gactccatcg agggaggccg cgctgcgtac cacattccct
    13141 ctgcactccc ccgatttcta cgcgggggac ccataccctg cgtacaagga gctgcgcgcg
    13201 agcgcgccgg tgtgctggaa cgacgtcacg aagttctggg cactgctgcg ctacgacgac
    13261 attcggtatg tgtccacaaa cccggacaag ttctcctcga ccgcgggcat caccatccca
    13321 gacccggtga tgccgaaccc ggttcaggag ggcgccctca tcttcaccga cccgccgcgg
    13381 catcgtcagc tgcgcaagct gatcaacacc gggttcactc ggcggcaggt ggccatcctc
    13441 gagccgaagg tgcgcgaaat cgtccacggc gtcttcgacg atgtgcgggc cggatccacg
    13501 cacgagttcg ccgaagagct cgcggcaccg ttgcccaccc ggatgatcgc cgagctgctc
    13561 ggcgcgccac ccgacgattg ggaaaagttc cgcagatggt ccgatgcgtg caccgggaat
    13621 gccgaccccg agatcgaaat ggattcgctg gtggccgtcg gcgagctgtt cgaatacttc
    13681 cagcagctga tcgccgtgcg gcgcaccgag ccgcgaaacg acatgctgtc ggtgttgacc
    13741 accgccgaag tcgacggggc caagctgacc gacgaagact tgctgaactt cgcgttcctg
    13801 ctgctcgtcg cgggtaacga aaccactagg aacctgatcg cgctgggaac gttggcgctc
    13861 atcgagcatc cggcagaggc cgccaagctt gtcgaggatc ccgggctgat accggcggcg
    13921 gtagaggaga tgctgcgctg gaccagcccg gtgacacaca tggcacgggc ggcaacggcg
    13981 gacatcgaga tccgcgggca acagatccgc cggggcgaca tggtcgtgat gctgtacggc
    14041 tccgccaacc gcgacgagga ggtcttcggt tccgacgcag aggaattcaa ggtcactcgc
    14101 catccgaatc cacatatcgc attcggctgt ggcgaacact cgtgtgtcgg cgcacagctg
    14161 gcccgcctcg aagcccgcgt gttcttcgag gtattgctgg gccgcttccc cgagctggag
    14221 cttgtcggcg atgtcgatcg gatgcgcgcg acgatggtgc ccggcgtcaa gcgaatgccc
    14281 gtgcgactcg gaacggcaag ctgagcatgt acctggactt cacaccggag cagaaac
//
LOCUS       DASRJA010000085         4884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3869673,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000085 DASRJA010000000
VERSION     DASRJA010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3869673"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFC01_03870"
     CDS             complement(<1..618)
                     /locus_tag="VFC01_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZN78817.1"
                     /translation="MARFPKPFEGSWTEHYPQLGTDPVSYEDSISPEFYEVERNAVFK
                     RAWLNVGRVEQLPRKGSYFTKEMKVVNTSIILVRTGSGEIKAYHNICRHRGNKLVWND
                     MPLEETSGVCRQFTCKYHAWRYDLDGNLTFVQQEGEFFDLDKSQYGLVPVHCDVWEGF
                     IFVNFAPEPEQSLRDFLGPMITGLAGYPFDQMTSRWYYRSEVKANW"
     gene            723..2168
                     /locus_tag="VFC01_03875"
     CDS             723..2168
                     /locus_tag="VFC01_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZN78818.1"
                     /translation="MHQDQLFIGGNWAKPSTDRRIEVISPHSEEPVAQVAAAGTADVD
                     TAVEAARVAFDTGSWSRQTPAERVDAVRRLAKVYGERRSEMAEVITSEVGAPISFSQR
                     TQVGLPAMMMSAFCDVAESYPWREARPGRYGADIHIHREPVGVVAAIVPWNMPQFLIT
                     TKLIPALLAGCTVVVKPAPESPLNALLLAEMIAEIGLPEGVVSVLPGDAAVGEHLVKH
                     PGVDKVSFTGSTAAGRAVAAACATDLRRVSLELGGKSAAIVLADADPGAVAAGVRSAS
                     LSNSGQICNALTRILVPDGRADEFTDALAAEMTSLVVGDPTDSATQVGPLVAQRQQQR
                     VRGYIESGRQQGARLVVGGAEMPDGLDRGWYVKPTLFDGADNSMRIAREEIFGPVLTV
                     IPYADDEDAVAIANDSDYGLAGSVWTDDTDRGLAIAARIRTGTFGVNQGYTMDPFAPF
                     GGMKASGYGRELGREGIDGYTDTKSIAVAGH"
     gene            complement(2169..3092)
                     /locus_tag="VFC01_03880"
     CDS             complement(2169..3092)
                     /locus_tag="VFC01_03880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN78819.1"
                     /translation="MTIRLGLQIPNFSYGTPVTELFPAVRAQVAEAEAAGFDTVLVMD
                     HFYQLPTLGEPDEPMLEAYTTLGALASATERVQLSTLVTGNTYRNPTLLAKEITTLDV
                     ISGGRAVLGIGAGWFELEHNQLGFEFSTFTERFEKLEEALQIIIPMTHGERPSFSGKW
                     YHTESAINEPRYRDHIPVMLGGSGEKKTFRLAAQYADHLNIIAPMDDLPGKLRALEQR
                     CAEANRDPATLETSCLLTVMVDGFESHGTMDEARGGRAVSGTPEQVAEEIKRRVLDVG
                     IDGIIVNLPTHRYTPGVITAVGEALKPLTTP"
     gene            complement(3150..3752)
                     /locus_tag="VFC01_03885"
     CDS             complement(3150..3752)
                     /locus_tag="VFC01_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN78820.1"
                     /translation="MVEQWTRERRIEHTRSLLLDAAEEVFAEKGFAPATLDDIAHAAG
                     YTKGAIYKHFATKEDLFLAVSDRYWRRYFDNFAEVMSSARQIGARELDEIAKRWRQLS
                     RDRGAEHAALGHEFTLYLLRNPDARERVAAKRSEVVEALAKFIVTGMDRLGAALLIPP
                     MTFAQLLIATSDAIVLSSELDDVDLYRPIVEMYTSAIKLP"
     gene            3824..>4884
                     /locus_tag="VFC01_03890"
     CDS             3824..>4884
                     /locus_tag="VFC01_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spirocyclase AveC family protein"
                     /protein_id="HZN78821.1"
                     /translation="MSELSNKPRAVVEPIPGAAALSDDVHSKVKPITIWAAIGGAVLV
                     LQLYVWIRWITGPYFERVPGGPTDPPLYMRAFLTFNGILVCVGLPVAIWWFIIRPWRR
                     ERRITLDGMLLVSMGLMFFQDPLLNYFNTWCTYNTWLWNRGSWSSDIPGWVSPEQPGR
                     QVAEPLLINAPGYTAGVLVITIFGCWVMRKIKARWPSITNLRLILVTYAFTFVLDFVM
                     EAGFMLPFGLYTYPGAIRSVSVFAGTYHQWPIYEGLMWGGVQAAMCCLRYFTDDRGRT
                     IVERGLDNVRGGFARQQFIRFLAIFAAISGCFFFFYNVPAQWIAMHSDPWPEDHLKRS
                     YFNGGICGDGTDVPCPDPAL"
BASE COUNT          847 a         1525 c         1557 g          955 t
ORIGIN      
        1 ccagttcgcc ttgacctccg agcggtagta ccaccgcgac gtcatctgat cgaacgggta
       61 gcccgcgagc ccggtgatca tcggcccgag gaagtcacgc aggctctgtt cgggttcagg
      121 tgcgaagttg acgaagatga agccttccca cacgtcgcag tgcaccggca ccaacccgta
      181 ctgactcttg tcgaggtcga agaactcccc ctcctgctgc acgaacgtga ggttgccgtc
      241 caagtcatag cgccaggcgt ggtacttgca ggtgaactgg cgacagacgc cgctggtctc
      301 ctcgagaggc atgtcgttcc acaccagctt gttgccgcgg tgcctgcaga tgttgtggta
      361 ggccttgatc tcaccggacc cggtgcgcac caagatgatt gacgtgttga cgaccttcat
      421 ttccttggtg aagtaactgc ccttgcgcgg aagttgttca acgcgaccaa cattgagcca
      481 tgcgcgcttg aacaccgcgt tccgttctac ctcgtagaac tcggggctga tggaatcctc
      541 ataggacacc ggatccgtcc ccagctgcgg gtagtgctcc gtccagctgc cttcgaacgg
      601 tttcgggaat cgagccatcg ccttctctcc ttctccactc ggtagcgccg acggtatatg
      661 ctccaggcaa caatcacaag tagttgatat tgccgtcgtg tcgttcggaa ggagacggtc
      721 gagtgcatca ggaccagctg ttcatcggcg gaaattgggc caagccgagc accgaccgcc
      781 gcatcgaggt gatctccccg cactccgaag aaccggtggc acaggtcgcg gccgcaggga
      841 ctgccgacgt ggacacggcg gtcgaggccg cacgggtcgc attcgatacg gggtcgtggt
      901 ctcggcagac accagccgag cgcgtcgacg ccgtgcgccg gcttgccaag gtgtacggcg
      961 aacggcggtc cgagatggcc gaggtgatca cctcggaggt cggtgctccg atcagcttct
     1021 cgcagcggac tcaggtaggg ctgcccgcga tgatgatgag tgcgttctgc gatgtggccg
     1081 agtcttatcc gtggcgggag gcgcggccgg gaaggtacgg cgccgacatc cacatccatc
     1141 gcgagccggt cggcgtcgtc gccgcgatcg tgccgtggaa catgccgcag ttcctgatca
     1201 ccaccaagct gatccccgcg ctgttggccg ggtgcaccgt cgttgtcaaa cccgcaccgg
     1261 aatcaccgct gaacgcgctg ctgctggcgg agatgatcgc cgagatcggg ttgccggagg
     1321 gcgtcgtcag tgtgctgccc ggtgacgctg ccgttggcga gcacctggtg aaacaccctg
     1381 gtgtggacaa ggtttcgttc accggttcga cggccgcagg tagggccgtc gcggccgcat
     1441 gcgcgaccga cctgcgccgc gtcagcctgg aactcggcgg caagtccgct gcgatcgtgc
     1501 tcgcggacgc cgatccaggc gccgtcgccg ccggcgtgcg gtcggcgagc ctgtcgaata
     1561 gcggccagat ctgcaatgcg ctgacccgca tcctggtgcc ggacggtagg gccgacgaat
     1621 tcaccgatgc actggccgcc gagatgacgt cactcgtcgt cggggacccg acggacagcg
     1681 cgacgcaggt gggtccgctc gtcgcgcagc gtcagcagca gcgcgtgcgc gggtacatcg
     1741 agagcggtcg gcaacagggc gcccggctag tcgtgggcgg ggccgagatg cccgacggcc
     1801 tggaccgggg atggtatgtg aagccgaccc tgttcgacgg tgccgataac tcaatgcgaa
     1861 tcgctcgcga ggagatcttc ggcccggtgc tgaccgtgat cccctacgcc gacgatgagg
     1921 acgcggtggc catcgccaac gactccgact acggcctggc cgggtccgtc tggaccgatg
     1981 acaccgaccg tggcctcgcg atcgccgcac gcattcgcac cggcaccttc ggcgtcaacc
     2041 agggctacac catggatccg ttcgcaccgt tcggcggcat gaaggccagc ggttacggac
     2101 gcgagctggg tcgcgaggga atcgacggct acaccgacac gaaatccatt gcggtggcgg
     2161 gacactgact acggtgtggt cagtggcttc agtgcctcgc caaccgcggt gatcacgccg
     2221 ggcgtatagc gatgggtcgg cagattgacg atgatgccat cgatgccgac gtcgaggacg
     2281 cggcgcttga tctcctcggc cacttgctcg ggtgtgccgg aaactgcgcg ccctccgcga
     2341 gcttcgtcca tcgtgccatg ggattcgaat ccgtcgacca tcaccgtgag caggcagctc
     2401 gtttccagcg ttgccgggtc gcggttggcc tccgcgcacc gttgttcgag cgcgcgtagc
     2461 ttcccgggaa ggtcgtccat gggcgcgatg atgttgaggt ggtcggcgta ctgggcggcg
     2521 agccggaagg tcttcttctc cccgctaccc ccgagcatca ccggaatgtg gtcacggtag
     2581 cgtggctcgt tgatggcgct ttcggtgtga taccacttgc ccgaaaagct gggccgctca
     2641 ccgtgagtca tcggaatgat gatctgcagt gcctcttcca gcttctcgaa acgctctgtg
     2701 aacgtgctga attcgaaacc cagctggttg tgctcgagct caaaccaccc ggccccgatg
     2761 cccaagaccg ctcgtccgcc actgattacg tccaaggtcg tgatttcctt ggccaacagg
     2821 gtcgggttgc gataggtgtt tccggtgacc agcgttgaaa gctgcacacg ctcggtggcg
     2881 ctcgccagcg cacccagtgt cgtgtaggcc tccagcatcg gttcgtcggg ctcccccagc
     2941 gtgggcagct gatagaagtg atccatcacc aaaacggtgt cgaatccggc agcttcggct
     3001 tctgcgactt gagctcgcac tgcggggaac agctctgtga cgggtgttcc gtatgagaaa
     3061 ttggggatct gcaatccaag tcgaatagtc acgcctcacg gtgacgactg ccgggcagga
     3121 tttattcccc ggcccgaacc ggctctaagt caaggaagct tgattgccga ggtgtacatc
     3181 tccacgatcg gacggtagag gtcgacgtcg tcgagttcgc tgctcaatac gatggcgtcg
     3241 ctggtggcga tgagtaactg cgcgaacgtc atcggtggaa tcagtaaggc tgcgccgagc
     3301 cggtccatgc cggtcacgat gaatttcgcc agcgcctcga caacctcaga cctcttggcc
     3361 gccacccgtt ctcgggcgtc ggggttgcga agcagataaa gggtgaattc atggcccagc
     3421 gcggcgtgct cggcgccgcg gtcacggctg agctggcgcc aacgcttggc aatctcgtcg
     3481 agctcgcgcg ctccgatctg cctggcggac gacatcacct cggcgaagtt gtcaaagtaa
     3541 cggcgccagt acctgtcgct caccgccaga aacaggtcct ctttcgtcgc gaagtgcttg
     3601 tagattgccc ccttggtgta gccggccgca tgcgcgatgt cgtcgagagt cgctggcgcg
     3661 aacccttttt cggcgaagac ctcttctgcg gcatcgagca gaagcgaacg cgtgtgctcg
     3721 atgcgtcgct cgcgcgtcca ttgctccacc atgcgtgcga ttgtgacaca ggatcttaga
     3781 aacttgtagg ttgctcagat actgagtggt atcttgacgc gtcatgagcg aactgtcaaa
     3841 caagccacgg gccgtcgtcg agccgatccc gggcgcggcg gcgctgagcg acgacgttca
     3901 cagcaaagtc aagccgatca ccatctgggc tgccatcggt ggcgcggtgt tggtgctcca
     3961 gctgtacgtg tggattcggt ggatcaccgg gccctacttc gagcgggtgc cgggcggccc
     4021 caccgacccg ccgctctaca tgagggcctt cctcacgttc aacggcatcc tcgtctgcgt
     4081 cggcctgccg gtcgccatct ggtggttcat catcaggccg tggcgtcggg agcggagaat
     4141 cacgctcgac ggcatgctgc tggtgtcaat gggcctgatg ttctttcaag acccgctact
     4201 caactacttc aacacgtggt gcacctacaa cacctggctg tggaaccggg gctcgtggtc
     4261 gtcggacatt ccgggatggg tctcacccga gcaacccggc cgacaggtcg ctgagccgct
     4321 cttgatcaac gctccgggat acacggccgg cgtactggtc atcaccatct tcggatgctg
     4381 ggtgatgcga aagatcaagg cgcgctggcc cagtatcacc aacttgcggc tgatactggt
     4441 cacctacgcc ttcacgttcg tgctcgactt cgtcatggaa gccggcttca tgctgccgtt
     4501 cgggctctac acctatcccg gcgcgattcg ctcggtgtcc gtgttcgccg gcacgtatca
     4561 ccagtggccg atctacgagg gattgatgtg gggcggcgtc caggcagcaa tgtgctgtct
     4621 gcgctacttc accgacgatc gcggccgcac gatcgtcgaa cgggggctcg acaacgtacg
     4681 agggggtttc gcgaggcagc agttcatccg ctttcttgcc atcttcgcgg cgatcagcgg
     4741 atgctttttc ttcttctaca acgtgcccgc gcaatggatc gccatgcact ccgacccgtg
     4801 gcccgaagac cacttgaagc gttcgtactt caacgggggc atctgcgggg acgggacgga
     4861 cgtaccctgc cccgaccccg ccct
//
LOCUS       DASRJA010000086         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_770241,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000086 DASRJA010000000
VERSION     DASRJA010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770241"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1142
                     /gene="selD"
                     /locus_tag="VFC01_03895"
     CDS             105..1142
                     /gene="selD"
                     /locus_tag="VFC01_03895"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HZN78822.1"
                     /translation="MAIAAGTPNRGSKTQVRLTQYAHGGGCACKIPPDELEAVVGGLQ
                     LDAPMLQAYGPLLVGLENGDDAAVVAAPDGGPAFVLTTDFFTPVVDDAYDWGRIAATN
                     ALSDIYAMGGRPVVAVNLLCWPREVLPMELAAEVLRGGLAVTSVAGCHLVGGHSVDDP
                     EPKYGLAVSGVVDPNRILRNDAGRVGDPLSLTKPLGIGVLNSRHKATGEVFEQAVAAM
                     TTLNRDAAAAAVERGYRCATDVTGFGLLGHVLKLARSSGVTAVIDSAAVAYLDGARMA
                     ADAGYISGGTRRNLDWVRPHTDLTGIDELEALLLADAQTSGGLLIAGEIPGADIIGEL
                     VPRGEHLVVVR"
     gene            1182..1276
                     /locus_tag="VFC01_03900"
     tRNA            1182..1276
                     /locus_tag="VFC01_03900"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1216..1218,aa:Sec,seq:tca)
     gene            1328..>2376
                     /gene="selB"
                     /locus_tag="VFC01_03905"
     CDS             1328..>2376
                     /gene="selB"
                     /locus_tag="VFC01_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005514720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HZN78823.1"
                     /translation="MHVIATAGHVDHGKSALLRALTGMEPDRWEEEQRRGLTIDLGFV
                     WTDSDRDPIAFVDVPGHERFVGNMLAGVGPVPAVLFVVAADEGWMPQSAEHLMALHAL
                     GVSHGVLAVTKADLMEPELAAEEALDRIRTTTLGEIPWVGVSARTGTGLADLCRALDD
                     LVARLPASDDSAPVRLWVDRSFTIRGAGLVVTGTLAAGTIRAGDTLELARTEARFVVR
                     EIQTLNRHVEDVAGVARVALNLRGPGRVQIRRGDALMTPDSYRCSAVVDVRTSLCTPA
                     DLPREMLMHIGAATVTCQVRPIGQDTARLTLDEALPVRVGDRVVLRHDRGIAGGAVLL
                     DVDPPVLNRRGAGRRR"
BASE COUNT          353 a          770 c          835 g          418 t
ORIGIN      
        1 cgagccggtc gacggccgcg gccagctccg gcatggcgag caggtggtcc gtagcgggga
       61 tgcgccgacg tgcgtctacg tcatccccgc ctgacaatgg ttgcatggcc atcgcagccg
      121 gcactcccaa tcgaggttcg aagacgcagg ttcgcctgac gcagtatgcc catggtggcg
      181 gttgtgcatg caagattcca ccggacgaac tcgaggccgt tgtcggcggg ctgcagctcg
      241 acgctccgat gctgcaggcg tacggtccgt tgctggtcgg ccttgagaac ggcgacgatg
      301 cggcggtggt cgcagcgccc gacggcggcc cggcgttcgt actgaccacc gacttcttca
      361 cgccagttgt cgacgacgcg tacgactggg ggcggatcgc ggctaccaac gcactctccg
      421 acatctatgc gatgggtggt cgacccgtcg tggcggtcaa cctgctctgc tggcctcgag
      481 aggtgctgcc gatggagctc gccgccgagg tgctgcgcgg cggcctcgcg gttaccagcg
      541 tcgccggttg ccatcttgtc ggcggacaca gcgtcgacga cccagagccg aagtacgggc
      601 tcgccgtttc tggcgtggtt gacccaaacc ggattctccg caacgacgcc ggccgggtcg
      661 gagatccgct ctccctgacg aagccgctcg gcatcggcgt gcttaattca cgccacaagg
      721 caacgggaga ggtcttcgaa caggcggtcg ccgcgatgac cacgttgaac cgcgacgccg
      781 cggcggcggc cgtcgagcgt ggataccgct gcgcgacaga cgtcaccggc ttcggcctac
      841 tcggccacgt gctcaagctc gcccggtcca gcggcgtcac ggcggtcatc gactctgctg
      901 cggtggcgta ccttgacggc gcccgaatgg cggcggatgc cggatacatc agtggcggca
      961 cccggcgcaa tctggactgg gtgcgccccc acaccgatct cacgggcatc gatgagcttg
     1021 aggcgctgct gctggccgac gcgcagacgt cgggcggctt gctcatcgcc ggcgagatcc
     1081 ccggagcgga catcatcggc gaacttgtac caaggggcga gcatctcgtc gtcgtccgct
     1141 gacctcttcc ccgacggcga tctaggttct agggtcgtcg cggaggtgtt tgtcggctgg
     1201 tgccggccgc ggccttcaaa gccgtagggc gtgtgacgaa cgcgccggcg ggttcgattc
     1261 ccgtccgcct ccgccagtcg tcgtcgagga cttgcacctc cttgaccgag cgagcggcgg
     1321 tacccggatg catgtgatcg ccacagctgg tcacgtcgac catgggaagt ccgcgctgct
     1381 gcgggctttg acgggaatgg agcccgatcg ctgggaggag gaacagcggc gcgggctgac
     1441 catcgatctc ggtttcgtgt ggacagacag cgaccgtgat cccatcgcct ttgtcgatgt
     1501 tcccggccac gagcggttcg ttgggaacat gctcgccggc gtgggtccgg tgcccgcggt
     1561 gttgttcgtg gtggctgcgg acgagggctg gatgccccaa tcggccgagc atctgatggc
     1621 tctgcacgcc ctgggcgtct cccacggcgt cttggccgta acgaaggccg acctcatgga
     1681 gccggagctc gccgcggagg aggcactcga ccggatacgg actaccacac tcggcgagat
     1741 cccatgggtc ggcgtgagcg cacgaacggg cacaggtctg gcggaccttt gtcgagcgct
     1801 tgacgacctc gtcgctcggc tgccggcgag cgacgattcg gcgccggtgc gattgtgggt
     1861 cgaccgcagc ttcactatcc gcggggctgg tcttgtcgtt actggaacgc tcgccgcagg
     1921 cacgataagg gccggcgaca cgttggagct cgcgcggacc gaggcgcgtt ttgtcgtgcg
     1981 tgagatacag acgctcaacc gccatgtcga ggacgtggcc ggtgtcgcca gggtggcgtt
     2041 gaatctgcgc ggtccggggc gcgtgcagat tcgacgtgga gacgcgctca tgacgcccga
     2101 ctcgtaccgc tgttccgcag tggtcgatgt ccgcacgtcc ctgtgcacgc cagcggatct
     2161 gccgcgcgag atgctgatgc acataggtgc tgcgacagtg acctgccagg tccggccgat
     2221 cggccaagac actgcgcggc tcacgctcga cgaggccctg ccggtccgcg tcggtgaccg
     2281 ggtggtgttg cgccacgatc gtggtatcgc cggaggcgca gtcctgctcg acgtcgatcc
     2341 gccggtcctc aatcgccgcg gagccggccg gcgtcg
//
LOCUS       DASRJA010000087         5650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3027831,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000087 DASRJA010000000
VERSION     DASRJA010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3027831"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VFC01_03910"
     CDS             <1..594
                     /locus_tag="VFC01_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016888515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZN78824.1"
                     /translation="TTETAGRPKIAGAAAIPGSDELVVYKTIDPLTGVELPPEQEGEL
                     VSNGPTVMQGFWNRPEQTAATLRDGWLHSGDVGVVRADGYLQVTGRSKELYKSGGELV
                     MPKEIEDLLAGHDAISQVYAIGLVDDRWGEIGCAVVVRVPGAELTEDEVLTICRAKLA
                     KFKVPKRVVFFDADELPKTPTGKVQKFKLAELLSFRR"
     gene            complement(584..1777)
                     /locus_tag="VFC01_03915"
     CDS             complement(584..1777)
                     /locus_tag="VFC01_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZN78825.1"
                     /translation="MGFRDKVATIATAGKIASSIVGERFLPQAPIKDPAQVPPSAEAI
                     TPDWLSSVLCSRVTSARVVDIRLSRGDNGTSARRAIDVVYNDAGTMAGLPTQLFSKST
                     ATLGSRMLLGVTGIAEGESVFYNHARPDLKLRSPHAYYSGYDPKSHRSLVLLEDLTVR
                     GWTFPDPMDNLVRRGDAEDMVTELALYHGALWDSPRFRTDLTRLRPAYDWQDNLNRKV
                     GFEKRTLTGLERAKDVVSPALYAQRDKLYPAFMRSLTLHHAAPQTLLHQDLHLGNWLR
                     DDDGRMGLYDWQCVAKGSWALDYSYAIGGSLTTADRREWQEDLLRLYVEQLRHHGAAT
                     APSFDEAWLAYRQQPMHALAFALFTYGGSRLEPELQPKDYTLASIERIAQHVTDLGSI
                     DALTA"
     gene            complement(1795..2865)
                     /locus_tag="VFC01_03920"
     CDS             complement(1795..2865)
                     /locus_tag="VFC01_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN78826.1"
                     /translation="MKIGMPISYAGDFRETMANLRDYESVGLDRIMVPEAYGFDAVTQ
                     LGYAAASTDRLELAFGILPMYSRSPTNLAMTAAGLDYVSNGRCILGLGASGPQVVEGF
                     HGVKYDAPLGRAREHVEICQKIWRRDKSEYHGEHYQLPLTEAAGGSGLGKPLRMMNHP
                     VRERIPMLLAAIGPKNVALAAEIFDEWQPFLFHPELAELAFGPALAAGRAKRDPALGE
                     LGISVQTFLLITDDPDEAAAALARVRAHVALYVGGMGAAGKNFYNSLFSRYGYPDEAE
                     SIQKLYLAGRKEDAAAAVPDAFAQSISLIGSIEHIGRRLEAYRLAGATCLIAEPLAGS
                     HAERVEQVAQLKILVPAAASVN"
     gene            3035..3490
                     /locus_tag="VFC01_03925"
     CDS             3035..3490
                     /locus_tag="VFC01_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZN78827.1"
                     /translation="MATVFGDLAELVSAVGADLGPTDWLEVDQKRIDQFAQATDDHQW
                     IHVDPERAVDGPYGGTIAHGLLTLSLLPHFMHLLYRVDNVAMAINYGFNKVRFITPVP
                     VGSRLRASSKLTAVTELAGGAQATLTTTIEVEGSDKPAAVVESIVRYVA"
     gene            3480..4700
                     /locus_tag="VFC01_03930"
     CDS             3480..4700
                     /locus_tag="VFC01_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019509854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN78828.1"
                     /translation="MWREVSVETRGDLASTAQALYQALAAGDRDSIDKLLSDDFVGHA
                     AEGLPLNMGGEHRGAEAMRRNLWWMIGKHFKAEAQPDEFRMLDDGGLLVAGHYRGVAR
                     HSGRRLDAEFTHVLAFDGDGRISSLRQLTDTAAWVEALGSGAPLETIDYSVSDGVATV
                     CLNRPDARNAIDLRVAEETLVVARRIADDPSVRAVLICGNGPALTVGGDIKYFLASPP
                     SEFGNLFSHMTAPFHEAFRILSRIDAPIVTAAHGSVAGGGLGYVYAADIVLAAEGTKF
                     VTAFAGIGLSGDGGGTWHLPRLIGPARATRAYLENRPITAEQALDWGLISEVVPADEL
                     RERAAALAASLATGPTRAFGRMRMLLRETWHNDLSQQLLAETEGVKATGDTVDAANAI
                     ASFAAKRKPQFEGR"
     gene            4703..>5650
                     /locus_tag="VFC01_03935"
     CDS             4703..>5650
                     /locus_tag="VFC01_03935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN78829.1"
                     /translation="MSGLIEDSVEHKAIRESVAGVVGRYGPEYFLERGRAGKDIEELW
                     KDLGAAGLLGVHLPEEYGGGGGGMAEAVVVVEELAAHGMPMLIWVISPAICGNILAHH
                     GSEAMKREWLPDLADGSRKMAFALTEPDAGSNSHKVATTARRTAAGWKISGAKYYISA
                     VDQADALLVVAKDAEHSTSEKNALSLFVVPTNSPGLQWQKIETSIVSPDSQFTVFLDD
                     VEIGEDALIGTAGNGLRQVFAGLNPERILVGAICGGVGRYAIGKAADYAKQRQVWSTP
                     IGAHQGIAHPLAESHIAVELSRLATMRSAELFDAGQSAAE"
BASE COUNT         1016 a         1739 c         1906 g          989 t
ORIGIN      
        1 accaccgaga ctgccgggcg gccgaagatc gccggcgcgg ccgccatccc cggatcagac
       61 gaactggtgg tctataagac catcgacccg ctcacgggtg tggagctgcc gccggagcag
      121 gagggtgagc tcgtctcgaa cggtccgacc gtgatgcagg gcttctggaa ccgaccggag
      181 cagaccgcag cgacgttgcg cgatggatgg ctgcactccg gtgacgtggg cgtggtgcgc
      241 gccgacggct accttcaagt cacgggccgc agcaaggagc tctacaagag tggtggcgag
      301 ctggtgatgc ccaaggagat cgaagatctc ctggccgggc acgacgcgat cagtcaggtg
      361 tacgcgatcg gcttggtcga cgatcgatgg ggcgagattg gttgtgcggt agtcgttcgc
      421 gtgcccggtg ccgagctcac cgaggatgag gtcctgacga tctgccgagc caagctggcg
      481 aagttcaagg tgcccaagcg ggtcgtcttc ttcgacgccg atgagctgcc caagacgccg
      541 acgggcaagg tgcagaagtt caaactcgcc gagctgctgt cgttcaggcg gtaagtgcgt
      601 cgatcgaccc gaggtccgtc acgtgttgcg caatgcgttc gatggacgcc aaggtgtaat
      661 ccttgggctg cagttcgggt tcgaggcggc tgccgccata ggtgaacagg gcgaatgcca
      721 aggcgtgcat cggctgctgc cggtaggcga gccaggcttc gtcgaatgac ggggcggtgg
      781 cggccccgtg atgcctcaac tgctcgacgt agagacgcag aaggtcttct tgccactcgc
      841 ggcggtccgc ggtcgtcaat gagcctccga tcgcgtacga gtagtccagt gcccagcttc
      901 ctttggccac gcactgccag tcgtagaggc ccattcggcc gtcgtcgtcg cgtagccaat
      961 tgccgaggtg gaggtcttga tgaagcagag tctgtggagc tgcgtggtga agcgtcagcg
     1021 atcgcatgaa ggcggggtac aacttgtccc gctgggcgta gagcgcgggg ctgacaacgt
     1081 ctttcgcccg ttccaggccg gtgagcgtgc gcttctcaaa accgaccttg cgattcaagt
     1141 tgtcctgcca atcataggcg ggacgcagcc gagtcaggtc ggtacggaac cgggggctgt
     1201 cccacagggc accgtggtac agcgccagct cggtgaccat gtcctctgcg tcgccgcgac
     1261 ggacgagatt gtccatcggg tcgggaaagg tccatccccg cacggtcaag tcctcgagga
     1321 gcaccagcga gcgatgcgac ttcgggtcgt agcccgagta atacgcgtgt gggctacgga
     1381 gcttcaagtc agggcgggca tggttgtaga agacggattc tccctcggcg atgccggtga
     1441 cgccgagcag catgcgtgaa ccgagagtcg ccgtcgactt agagaacaac tgggtcggaa
     1501 gcccggccat cgtgccggcg tcgttgtaga cgacgtcgat ggcgcggcgt gcgctcgtgc
     1561 cgttgtcacc tcgcgacagc ctgatgtcaa ccacgcgcgc actggtgact cgggaacaca
     1621 acaccgatga gagccagtcc ggcgtgatcg cctccgccga gggcgggacc tgcgcggggt
     1681 ccttgatggg agcctgtgga aggaacctct ccccgacgat tgacgaggcg atctttccgg
     1741 ctgtagcgat cgtcgcaacc ttgtcgcgaa aacccatgtc gaggttccct tcagtcagtt
     1801 cacgctcgcc gctgcgggaa cgaggatctt cagttgcgcc acttgctcga cgcgctcggc
     1861 gtggcttcct gccaacggtt cggcgatcaa acaggttgcc ccggctaggc gatacgcctc
     1921 caggcgccgc ccgatgtgct cgatcgaccc gatgagggaa atcgactggg cgaaggcgtc
     1981 tggcaccgcg gccgccgcat cttccttgcg cccggcgagg tagagctttt ggatcgactc
     2041 cgcctcatcg ggatagccgt aacgggagaa cagcgagttg tagaagttct tgcccgcggc
     2101 gcccatgccg ccgacataga gcgcgacgtg ggcgcgcacg cgcgccaggg ccgcggccgc
     2161 ctcgtcgggg tcatcggtga tcagcaggaa ggtctgcacg gagatgccta gctcacccag
     2221 cgcaggatcg cgcttggccc gcccggcggc caaggccggt ccaaacgcca gctcggccaa
     2281 ctcgggatgg aacaggaacg gctgccattc gtcgaagatc tcagcggcca gcgcaacgtt
     2341 cttcggtccg atcgctgcga gcagcatggg aatgcgttcc cgcacggggt gattcatcat
     2401 gcgcagcggc tttcccaggc cgctgccgcc ggctgcctcg gtgaggggga gttgatagtg
     2461 ctcaccgtgg tactcgctct tgtctcggcg ccagatcttt tggcagatct ccacgtgctc
     2521 gcgtgcgcga ccaagcggtg cgtcgtactt cacgccgtga aatccctcga cgacctgtgg
     2581 accggatgcc ccgagtccga ggatgcaccg tccattgctg acgtagtcga gtcccgccgc
     2641 ggtcattgcg aggttggtgg ggctacggga gtacatgggc aggatgccaa aggccaactc
     2701 gagccgatcg gtcgacgcgg ccgcgtagcc caactgggtg acggcatcga acccgtaggc
     2761 ctcgggcacc atgatgcggt ccaagccgac ggactcgtag tcacgcaggt tggccatggt
     2821 ctcgcggaaa tcaccggcgt agctgatggg catgccgatc ttcatctgtc gcctccgagg
     2881 tccggtctga atgtttcgat gatgcggcat aatttaacat gcaatagaaa gactgttgac
     2941 atgtgcgtag cagcgccttt ggtatatggt gcgccatcgt ctaatctata ttaaatagtt
     3001 agtgtactgg atcgcgacga tgcggaggtg gacggtggcg acggtattcg gtgacctggc
     3061 cgaactagtg agtgcggtgg gcgcagacct aggccccacc gactggctgg aggtggacca
     3121 gaagaggatc gaccagttcg cccaagccac cgacgatcac caatggattc acgtcgaccc
     3181 cgaacgtgcc gtcgacggtc cctacggagg caccatcgcg cacgggctgc tgacgctgtc
     3241 gttgttgcca cacttcatgc atctgctgta tcgggtggac aacgtcgcca tggcaatcaa
     3301 ctacggcttc aacaaggttc ggttcatcac gccggtgccg gtcgggtcac ggctgcgtgc
     3361 gtcctcgaag ctgaccgcgg tgaccgagct tgccggaggc gcccaggcaa cgctgaccac
     3421 cacgatcgag gtcgaggggt cggacaagcc cgctgctgtc gtcgagtcca tcgtccgata
     3481 tgtggcgtga ggtgagcgtg gagactcgcg gcgaccttgc atcgacggca caggcgctct
     3541 accaggcctt ggcggctggc gatcgcgatt cgatcgacaa gctactcagt gacgacttcg
     3601 tcgggcacgc cgcggagggg ttgccgctga acatgggtgg ggaacaccgc ggtgcggagg
     3661 cgatgcggcg caacctctgg tggatgatcg gcaagcactt caaggccgag gctcaacccg
     3721 acgagtttcg gatgctcgac gacggcggcc ttctggtggc cggtcactac cgaggggttg
     3781 cgcgccattc gggacggcgg ttggatgccg aattcacgca cgtgcttgcg ttcgacggcg
     3841 acggccggat cagctcactg cgccaactga ccgacaccgc cgcctgggtc gaggcgttgg
     3901 gctctggcgc gccgctcgag acgatcgact actcggtgtc cgacggtgtc gccaccgtct
     3961 gcttgaacag acccgacgca cgcaatgcca tcgacctacg cgtagcggag gagacgttgg
     4021 tcgtcgcccg ccgcatcgcc gatgatccgt ccgttcgcgc cgtgctcatc tgcggcaacg
     4081 ggcccgcgct gaccgtcgga ggggacatca agtacttcct cgcgtcaccg ccgtcggaat
     4141 tcggaaacct gttctcccac atgacggcac cgttccacga ggcgtttcgc attctgagcc
     4201 ggatcgatgc cccgatcgtc accgccgctc acggatcggt ggccggtggc gggctggggt
     4261 acgtctacgc ggccgacatc gtgctggccg ccgaagggac caaattcgtc acggcgttcg
     4321 caggaatcgg gctctcggga gacggcggcg gcacctggca tctcccacgg ctcatcggac
     4381 cagcccgtgc cacccgcgcc tacctggaga atcgcccgat caccgccgag caggcgctcg
     4441 actggggatt gatcagcgag gtcgtgcccg ccgacgaact ccgcgaacga gcggccgcac
     4501 tggccgcgtc gctggccaca ggccccaccc gagccttcgg ccggatgcgc atgcttctgc
     4561 gggagacctg gcataacgac ctgtcccaac agctgttggc cgagaccgag ggagtcaagg
     4621 ccaccggcga caccgtcgac gccgccaacg ccatcgccag cttcgccgcg aagcgcaaac
     4681 cccagttcga aggaaggtag acatgtcagg actcatcgaa gactcggtgg aacacaaggc
     4741 tattcgggag agcgtcgcgg gcgtcgtcgg gcgttacggg cccgagtact tcctggaacg
     4801 cggccgcgcc ggcaaagaca ttgaagagct gtggaaggac ctcggcgccg cgggtctgct
     4861 tggcgtccac ctgcccgagg agtacggcgg cgggggcggt ggcatggccg aggccgtcgt
     4921 cgtcgtggag gaactggcag cccacggaat gccgatgctg atctgggtga tttcgccggc
     4981 gatttgcggc aacatcctcg ctcaccacgg ttctgaggcg atgaagcggg aatggctgcc
     5041 cgacctcgcc gacggatcgc gcaagatggc gttcgcgctg accgagcccg atgccgggtc
     5101 gaacagccac aaggtcgcca ccaccgcgcg gcgcaccgct gcgggttgga agatctccgg
     5161 ggcgaagtac tacatttcgg ccgtcgatca ggccgacgcg cttctggtcg tggccaagga
     5221 cgcggaacac tcgacgagcg agaagaacgc gttgtcgctg ttcgtggtgc ccacgaacag
     5281 tccaggcctg cagtggcaaa agatcgagac gtcgatcgtg tctccggaca gccaattcac
     5341 cgtgttcctc gatgacgtcg agatcggcga ggacgctcta atcggcacgg caggcaatgg
     5401 tctgcggcag gtgttcgccg ggctcaaccc cgagcgcatc ctggtgggtg ccatctgcgg
     5461 tggggtcgga cggtatgcca ttggcaaggc ggcggactac gccaaacaac gccaggtgtg
     5521 gtccaccccg atcggtgcac accaggggat cgctcatccg ttggcggagt cacacatcgc
     5581 cgtagagctc agccggctgg ccaccatgcg aagtgccgaa ctattcgacg ccggtcagtc
     5641 ggcagccgaa
//
LOCUS       DASRJA010000088        10422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3027661,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000088 DASRJA010000000
VERSION     DASRJA010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10422
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3027661"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VFC01_03940"
     CDS             <1..1091
                     /locus_tag="VFC01_03940"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZN78830.1"
                     /translation="IVSKALRPLPVAHKEMSEESRVRQRYVDLIIRPEARSIARQRVA
                     VVRAARDALHRRGFLEVETPMLQTLAGGAAARPFVTHSNALDADLYLRIAPELFLKRC
                     LVGGFDRVFELNRVFRNEGADSTHSPEFAMLETYQAYGTYDDSAIMTREVIQEVADDA
                     IGTRRLPLPDGTIYDLDGEWETIQMYPSLSEALGEQITPETPAERLWQIADRLGVEIP
                     RDRGYGHGKLVEELWEHSVGNKLWAPTFVRDFPVETTPLTAPHRSIEGATEKWDLYIR
                     RIELATGYSELIDPVIQRERFEAQARAAAAGDDEAMVLDEDFLAALEYAMPPSTGTGM
                     GIDRLLMALTGLSIRETVLFPIVRPHIN"
     gene            1206..1547
                     /locus_tag="VFC01_03945"
     CDS             1206..1547
                     /locus_tag="VFC01_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HZN78831.1"
                     /translation="MAKKVTVTLVDDFDGEGAADETVEFGLDGVSYEIDLSSKNAAKL
                     RNDLKQWVEAGRRVGGRRRGRSAGSGRGRAAIDREQSAAIREWARRNGHNVSTRGRIP
                     ADVIDAFHAAT"
     gene            1835..4384
                     /gene="clpC1"
                     /locus_tag="VFC01_03950"
     CDS             1835..4384
                     /gene="clpC1"
                     /locus_tag="VFC01_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpC"
                     /protein_id="HZN78832.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKSLESLGISLEGVRSQVEEIIGQGQQAPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGAELTRVRQQVIQLLSGYQGKETAEAGTGGRGG
                     EAGNPSTSLVLDQFGRNLTAAAMEGKLDPVIGREKEIERVMQVLSRRTKNNPVLIGEP
                     GVGKTAVVEGLAQAIVHGEVPETLKDKQLYTLDLGSLVAGSRYRGDFEERLKKVLKEI
                     NTRGDIILFIDELHTLVGAGAAEGAIDAASILKPKLARGELQTIGATTLDEYRKYIEK
                     DAALERRFQPVQVGEPTVEHTIAILKGLRDRYEAHHRVSITDGAIAAAATLADRYIND
                     RFLPDKAIDLIDEAGARMRIRRMTAPPDLRDFDEKIAEARREKESAIDAQDFEKAASL
                     RDKEKQLVAQRAEREKQWRSGDLDVVAEVDDEQIAEVLGNWTGIPVFKLTEEETTRLL
                     RMEEELHKRIIGQEDAVTAVSKAIRRTRAGLKDPRRPSGSFIFAGPSGVGKTELSKAL
                     AEFLFGDQDALIQIDMGEFHDRFTASRLFGAPPGYVGYEEGGQLTEKVRRKPFSVVLF
                     DEIEKAHQEIYNSLLQVLEDGRLTDGQGRTVDFKNTVLIFTSNLGTSDISKAVGLGFT
                     KGGGDNNYERMKQKVNDELKKHFRPEFLNRIDDVIVFHQLTTDQIVRMVDLMIGRVEK
                     QLKSKDMAMQLTDKAKSLLAKRGFDPVLGARPLRRTIQREIEDALSEKILFEEVGPGQ
                     LVTVDVDNWDGEGSGEDAVFTFTGTKKPAKPAEPELEKAATAGAPTAAASE"
     regulatory      4464..4584
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01482"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The adenosylcobalamin (AdoCbl) riboswitch is found
                     in the 5'-untranslated regions of vitamin B12 (Cobalamin)
                     related genes in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01482"
     gene            4593..4808
                     /locus_tag="VFC01_03955"
     CDS             4593..4808
                     /locus_tag="VFC01_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB domain-containing protein"
                     /protein_id="HZN78833.1"
                     /translation="MTSPEARKTVAPAVDLSVAKAAVWLTLTAFLALIVLYFVGLDQG
                     ATSVFGDNMYVHEFVHDARHLLGFPCH"
     gene            4828..5553
                     /locus_tag="VFC01_03960"
     CDS             4828..5553
                     /locus_tag="VFC01_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HZN78834.1"
                     /translation="MEKRIIARGLMAGAIGGVLAFLFARTFVEPVIARALEFEEAHEQ
                     GHDHGVELFTRGVQANVGMGFGVLAFSVAMGALFAVVYCVAYGRVGNLSPRLLSVLLA
                     GGMFLSLYVIPFLKYPPSPPAVSLEETIRQRTLLYLLMVVLSAALLVAAVYLGHRLVA
                     QFGHWSATLIGAGCYVVAISLVMLMLPTIDETPDDFPADVLYEFRLYSLGTQLVLWAI
                     IGLVFASMAHRLFGERRRETVSA"
     gene            5650..6201
                     /locus_tag="VFC01_03965"
     CDS             5650..6201
                     /locus_tag="VFC01_03965"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZN78835.1"
                     /translation="MSEVVRLILVSHAMTDAMAAGRFPTDEPLNALGHRQVDAAIDLG
                     VTERAYCGPEKRTRQTAELLGLHASVDTRLADLDCGRWRGDVVGGVQSDDLAIWLTDP
                     ARAPHGGESVVELIDRVRGWMDSLTTRRIRLVAVTHPAVIRAAILIALNAPPKSFWRI
                     DIAPVSRTVVHFRGHAWTLRSGL"
     gene            complement(6202..7563)
                     /locus_tag="VFC01_03970"
     CDS             complement(6202..7563)
                     /locus_tag="VFC01_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZN78836.1"
                     /translation="MQGPAPRRSAGRARRTAAGLAVALVVAQACGCTNTGPAPVADAA
                     YGVPIDTNTPQGLRAKQTMDMVNSDWPIGTVGVRTMAIPEAVNGITSAMGNLWVDRPI
                     TVAGIEVGAGVATLHTLTPYGAAQDIEIHTNHANMVDRFAVELRPPAIRKWSDIDAEL
                     TKSGARYSYQVSKVTDGNCSTVAGSNTDLSLPLASIFKLYVLLAVADAIKAGTVSWTD
                     QLTITEEAKAVGSAGLEELPPGAQVSVRKAAQEMISASDNMATDLLIARLGPGAVERA
                     LVAAGHHDPASMTPFPTMHELFSVGWGEPDLREQWKHASRQDRVKMLQQANSRPFQPD
                     PNRTHTPASPDGVEWYGSAADICRVHAALQAAAVGKAAPVKQILATIPGIELDKAKWP
                     YIGAKGGNLPGDLTFSWYAVDHAGQPWVVSFQLNWPHFYSQTAAAWLLSIAHRAFDLA
                     PVA"
     gene            complement(7538..7855)
                     /gene="mhuD"
                     /locus_tag="VFC01_03975"
     CDS             complement(7538..7855)
                     /gene="mhuD"
                     /locus_tag="VFC01_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycobilin-forming heme oxygenase MhuD"
                     /protein_id="HZN78837.1"
                     /translation="MPVVKINAIEVPPDAGPELEKRFAHRAHAVDNQPGFLGFQLLRP
                     IKGEDRYFVVTQWESEEAFQAWATGPAIEAHAGQRANPVATGASLLEFEVVLDVAGSG
                     AKA"
     gene            7894..8664
                     /locus_tag="VFC01_03980"
     CDS             7894..8664
                     /locus_tag="VFC01_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN78838.1"
                     /translation="MHPDLLTRCGGDGDPLVLVHGLMGRGGTWTRQVPWLTRLGQVYT
                     YDAPWHRGRDVIDPYPVSTERFVSDLGDAVAELGRPAVMVGHSMGALHSWCLAADRPD
                     LVSALVVEDMAPDFRGRTTGPWEPWLHALPAEFDSAQKVYDEFGQVAGQYFLEAFDRT
                     ATGWRLHGHTRYWIDIAAEWGTRDYWKQWEAVRVPALLLEAGNSVTPAGQMRKMQETG
                     YRTTYLHVPGAGHLIHDEQPQIYQEAVESFLAALADGT"
     gene            complement(8638..8892)
                     /locus_tag="VFC01_03985"
     CDS             complement(8638..8892)
                     /locus_tag="VFC01_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78839.1"
                     /translation="MTTALTFLILIAPFAVAAALSWAAHRNDSLRIRFDQFRWSAPMM
                     GRLFEDDRDGFRVAHDVDAVRTRFEKQPFWPASGAVGERR"
     gene            8964..9950
                     /locus_tag="VFC01_03990"
     CDS             8964..9950
                     /locus_tag="VFC01_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN78840.1"
                     /translation="MALKSVSALVLDGLAIFEFGVICEVFGIDRSADGVPNFDFKVCG
                     PEPGKPLRTSVGASITPDHGLDDLVGADLVAIPAISSQYGGYLPEALDAVRKAADSGS
                     IILTVCSGAFVAGAAGLLDGRPCTTHWMHADDLARMYPTAKVDRNVLFVDDGNLITSA
                     GTAAGIDACLHLVRRELGSEVTNKIARRMVVPPQRDGGQRQYIDQPIPVRCSEGFAPH
                     LDWILTNLDRPHTVATLATRAHMSARTFARRFVEETGRTPMQWVTDQRVLYARRLLEE
                     SDLDIDRIADRSGFGTATLLRHHFRRIIGVTPSDYRRRFSCSGPCEEAAATA"
     gene            complement(9947..10225)
                     /locus_tag="VFC01_03995"
     CDS             complement(9947..10225)
                     /locus_tag="VFC01_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005081381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78841.1"
                     /translation="MNKLAIATATASLFAAGAIGLAPTAAAIPTGGGSAADTITTLQD
                     MGYAVQINGTVTGPLAQCIVTGVHGVSNSAPAVFTTAYVDVSCPSSDD"
     gene            complement(10276..>10422)
                     /locus_tag="VFC01_04000"
     CDS             complement(10276..>10422)
                     /locus_tag="VFC01_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014588448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN78842.1"
                     /translation="EVLVSSTVRDIVTGSRRHFTERGEYDLKGVPGHWRVHTLIRENA
                     PAKR"
BASE COUNT         1857 a         3235 c         3497 g         1833 t
ORIGIN      
        1 aaattgtttc caaggcgttg cgaccgcttc ctgttgcaca taaagagatg agcgaagagt
       61 cccgtgtccg gcagcgctat gtcgacctga tcattcgtcc ggaggcccgc agcattgctc
      121 gccagcgcgt ggccgtggtg cgcgcagcgc gtgacgcgct ccataggcgc ggttttctcg
      181 aagtcgagac gccaatgctt cagacgctgg cgggtggtgc cgcggcgcgt ccgttcgtca
      241 cacactccaa tgcgctcgat gcggacctgt accttcgcat cgcgccggaa ctgttcctaa
      301 agcgttgctt ggtaggcggt ttcgacaggg tcttcgagtt gaatcgagtg ttccgaaacg
      361 aaggtgcgga ttccacacat tcaccggaat ttgcgatgtt ggagacatat caggcgtacg
      421 gcacctacga cgattccgcg atcatgactc gcgaggttat tcaagaggtc gccgacgacg
      481 cgatcggcac cagaaggctg ccattgccgg atggcactat ctacgatctt gacggcgaat
      541 gggagacgat acaaatgtat ccgtcgctgt ccgaggcgct cggtgaacag atcactcccg
      601 agactcccgc ggagcggtta tggcagattg ctgaccggct cggcgttgag attccgcgcg
      661 accgtggtta cgggcacggg aaattggtcg aagaactgtg ggagcactcc gtcggtaaca
      721 agctgtgggc acctaccttc gtgcgcgatt ttccggtcga gacgacgccg ttgaccgcgc
      781 ctcatcgcag cattgagggc gctaccgaga agtgggatct gtacatccga cgcatcgagt
      841 tggcgaccgg ctactccgaa ctgatcgacc cggttatcca acgggaaagg ttcgaagccc
      901 aggcacgtgc ggcggccgcc ggtgacgacg aggcaatggt tctcgacgag gattttctgg
      961 cggctttgga gtatgcgatg ccgccctcta cgggcaccgg aatgggtatc gatcggttgt
     1021 tgatggcttt gacgggactg tcgattagag agacagtttt gttcccgatt gttcgtccac
     1081 acatcaactg aactgctccg attcagaact tgttgaatgg tgtgccttga tatggcacat
     1141 tggtgccggg gttcgagaat ttttgtactc atgtagtgct ctggcagaag ggtcagtgtg
     1201 ggctaatggc gaaaaaagtg accgtcacgt tggtcgatga tttcgacggc gaaggcgccg
     1261 ccgatgagac ggtcgaattc gggctcgacg gggtgagcta tgagatcgac ctttcttcca
     1321 aaaatgctgc aaagcttcga aacgacctga agcaatgggt cgaggcgggc cgccgggttg
     1381 gtggtcgtcg gcgcgggcgt tcagctggat cgggccgggg gcgagcggcg atcgatcgcg
     1441 aacaaagtgc cgcgatccgc gaatgggctc ggcgcaatgg acacaacgtg tccacccgtg
     1501 gtcgcatccc ggctgacgtc atcgacgctt tccacgcggc aacgtaatcg gcagttgcca
     1561 aaccatcgcg taggcgaact tcgccgctaa ctgctttcgc tagcggcgaa ctccctgcgt
     1621 cgcgagctcg gaaacgaatt ctccagcagc ggcgttgcta tgagcagcgc ctggtacagg
     1681 cccaaggcgc caacagccgt cactacatcg ctttctggac agaaccggca ggcggcgcgg
     1741 tggcggcagt cgcccactag acttgggtga cgggttcggg cgcggcgtgc cgcaaagaac
     1801 ccggcctatt ttgatggaga gcaggtaacc accgatgttt gagagattca ccgaccgtgc
     1861 ccgcagggtc gtcgtcctgg ctcaagaaga agcccggatg ctcaaccaca actacatcgg
     1921 caccgagcac atcctgttgg gacttattca tgagggtgag ggtgtagccg ccaagtcgct
     1981 ggagtcgctt ggcatctcgc tggaaggcgt gcgcagccag gtcgaggaga tcatcggcca
     2041 aggccagcag gcgccgtccg gtcacatccc gttcacccca cgcgccaaga aggtgctgga
     2101 gctgagcctg cgcgaggcgc tgcagctcgg ccacaactac atcggcaccg agcacattct
     2161 gctcggcctg attcgcgagg gcgaaggcgt cgcggcccag gtgttggtca agctgggcgc
     2221 tgagctgacc cgggtgcgtc agcaggtcat ccagctgctg agcggctacc agggcaagga
     2281 aaccgcggag gccggcaccg gcggccgcgg cggcgaggcg ggcaacccgt ccacctcgct
     2341 ggtgctcgac cagttcggcc gcaacctgac cgcggccgcc atggagggca agctcgaccc
     2401 ggtcatcggc cgtgagaagg aaatcgagcg ggtgatgcag gtgctgagcc gtcgcaccaa
     2461 gaacaacccg gtgctgatcg gtgagcccgg cgtcggcaag accgccgtcg tcgaggggct
     2521 tgcccaggcg atcgtgcacg gcgaggtgcc ggagacgctg aaggacaagc agctctacac
     2581 gcttgacctg gggtccttgg tcgctggctc aaggtaccgc ggtgacttcg aggagcgcct
     2641 gaagaaggtg ctcaaggaga tcaacacccg cggcgacatc atcctgttca tcgacgagct
     2701 gcacacgctg gtcggtgccg gtgccgccga gggcgcgatc gacgcggcca gcatcctcaa
     2761 gccgaagctg gcccgtggtg agctgcagac gatcggtgcg accaccctcg acgagtaccg
     2821 caagtacatc gagaaggacg ccgcgcttga gcgccggttc cagccggtgc aggtcggcga
     2881 gccgacggtc gagcacacga tcgcgatcct caagggattg cgtgaccgct acgaggcgca
     2941 ccaccgggtg tcgatcaccg acggcgcgat tgccgccgcc gccaccctgg ccgaccgcta
     3001 catcaacgac cggttcctgc cggacaaggc gatcgacctt atcgacgaag ccggcgcccg
     3061 gatgcggatc cgtcggatga ccgcgccgcc agacctgcgc gacttcgacg agaagatcgc
     3121 cgaggcgcgc cgcgagaagg agtccgcgat cgacgcgcag gacttcgaga aggcggccag
     3181 cctgcgcgac aaggagaagc aactcgtcgc gcagcgcgcc gaacgcgaga agcagtggcg
     3241 ctcgggcgat ctcgatgtgg tcgccgaggt cgacgatgag cagatcgccg aggtgcttgg
     3301 caactggacc ggcatccccg tgttcaagct gaccgaggag gagaccactc ggctgctgcg
     3361 catggaggag gagctgcaca agcggatcat cggccaagag gatgccgtca cggcggtctc
     3421 aaaggcgatc cggcgtaccc gcgccgggct gaaggatccg cggcgcccgt cgggctcgtt
     3481 catcttcgcc ggcccgtccg gtgtcggtaa aacggagctg tccaaggcgc tggcggagtt
     3541 cctgttcggc gaccaggacg cgctcatcca gatcgacatg ggcgagttcc acgaccgctt
     3601 caccgcctcg cgactgttcg gtgcccctcc gggctacgtc ggctacgagg agggcggcca
     3661 gctcaccgag aaggtgcggc gcaagccgtt ctcggtggtt ctgttcgacg agatcgagaa
     3721 ggcgcaccag gagatctaca acagcctttt gcaggtcctg gaggacggcc gcctgaccga
     3781 cggtcagggc cgcacggtcg acttcaagaa cacggtgctg atcttcacgt cgaacctggg
     3841 cacatccgac atcagcaaag cggtcggcct ggggttcacc aagggcggcg gcgacaacaa
     3901 ttacgagcgg atgaagcaga aggtcaacga cgagctgaag aagcacttcc gccccgagtt
     3961 cctcaaccgc atcgacgacg tcatcgtctt ccaccagctg acgaccgacc agatcgtccg
     4021 gatggtggac ctgatgatcg gccgggtgga gaagcagctc aagagcaagg acatggccat
     4081 gcagctgacg gacaaggcca agtcgctgct cgccaagcgc gggttcgacc ccgtgctcgg
     4141 tgcgcggccg ctgcgtcgga ccatccagcg cgagatcgag gatgcgctgt cggagaagat
     4201 cctcttcgag gaggtcggac cgggccagct cgtcacggtc gatgtcgaca actgggacgg
     4261 cgaaggctcc ggcgaggacg cggtgttcac gttcaccgga accaagaagc cggccaagcc
     4321 ggccgagccg gaactggaga aggcggccac cgccggcgcg ccgactgcgg ccgcttcgga
     4381 gtaagcacga caacacggaa aggtccccgg aatctagcgg ttccggggac ctttgcgtct
     4441 gttcgccaga ccttctgctt aggatgctga tgtaatcgac gggcgaagga atctggtgag
     4501 aatccagaac ggtcgcgcca ctgtgacagt cagacccgac gcccgtcgtc atccagtgaa
     4561 actgggacgc gaaatcccgg aaaggaactg acatgacctc tcccgaggcc cgtaagaccg
     4621 tagcgccggc agtcgacctc tcggtggcca aggcggcggt gtggctgacg ttgacggcgt
     4681 ttctggcgtt gatcgtgctc tacttcgtcg gcctggacca gggtgcgacg tcggtgttcg
     4741 gcgacaacat gtatgtgcac gagttcgtgc acgacgcccg ccacctgctc ggcttcccct
     4801 gccactgagc gccggcggcc aattcacatg gaaaaacgaa taatcgcgcg cggtctgatg
     4861 gccggcgcaa ttggtggcgt gctggccttc ttgttcgcgc gcacatttgt cgagcccgtc
     4921 attgcccgtg cacttgagtt tgaggaagcg cacgaacaag gccacgacca tggcgtcgaa
     4981 ttattcaccc gcggagtgca ggccaacgta gggatgggct tcggcgtgct ggcgttcagc
     5041 gtggcgatgg gcgccctgtt cgcggtcgtc tactgcgtgg cctacggccg cgtcggtaac
     5101 ctgtctccgc ggctgctgtc ggtgctactg gccggcggaa tgttcttgtc gctctatgta
     5161 attccgttcc tgaagtaccc gccaagtccg ccggccgtca gcctcgagga gaccatccgg
     5221 cagcgcaccc tgctgtactt gttgatggtg gtgctgtccg cggcgctact ggtcgcggcg
     5281 gtctacctgg ggcatcggct ggtagcgcag ttcggccatt ggagtgcgac cctgattggg
     5341 gcggggtgct acgtcgtggc catctcgctc gtcatgctga tgctgccgac catcgatgag
     5401 acgccggacg actttcccgc cgacgtcctc tacgagttcc ggttgtattc gctgggcacg
     5461 cagttggttt tgtgggcgat catcgggctg gtgtttgcgt cgatggcgca ccggctgttc
     5521 ggtgagcgtc gacgggagac agtctcggcg tgagtcccga aggcgagctc gagtgaggat
     5581 cgcagcgagg gacgagcgag gaccggtggg cccacgcccg cagggcaggg gacgagcggg
     5641 agccgaggca tgagtgaggt cgtccggctc atcctcgtat cgcatgcgat gaccgatgcg
     5701 atggcagccg gacgatttcc caccgacgag ccgctcaatg cactcggcca ccggcaggtc
     5761 gacgcggcca tcgacctggg cgtgaccgaa cgtgcctact gcggacccga gaagcggacc
     5821 aggcagacgg ccgaactgct cgggctgcac gccagtgtcg acaccaggct ggccgatctg
     5881 gactgcggcc gctggcgtgg cgatgtggtg ggcggggtgc agtccgacga tctggcaatc
     5941 tggctgactg atccggcgcg ggcgccgcac ggtggcgaat cggtcgtcga gctgatcgac
     6001 cgggtgcgcg ggtggatgga ctcgctgacc acccgtcgca tcaggctggt cgccgtcact
     6061 catccggcgg tgatccgcgc cgcaatcctg attgcgctca atgcaccgcc gaagtcgttc
     6121 tggcgcatcg acattgctcc cgtcagccgc acggtggtgc acttccgtgg acacgcctgg
     6181 acgctgcgct ccggcttgtg atcaggccac cggcgcgagg tcgaacgccc gatgcgcgat
     6241 cgacagtagc caggccgctg ccgtctggct atagaagtgc ggccaattca attggaagct
     6301 gaccacccac ggttggccgg cgtggtccac cgcgtaccaa ctgaatgtca agtcgccagg
     6361 caggtttccg cccttggcac cgatgtaagg ccacttcgct ttgtccagct cgataccggg
     6421 gatggtggcg aggatctgct tgaccggcgc cgccttgccg accgccgcag cctgcagcgc
     6481 ggcgtgcacc cggcagatgt cggccgcgct gccgtaccac tccacgccgt ccggagatgc
     6541 cggagtgtgc gtgcggttcg gatccggttg gaacgggcgg gaattcgcct gctgcagcat
     6601 cttcacccga tcctgccgag aggcgtgctt ccactgctca cgcagatccg gctcgcccca
     6661 accgacggag aacagttcgt gcatagttgg gaacggcgtc atgctggccg gatcgtggtg
     6721 cccggccgcc accagcgcac gctcgacggc gccgggcccc agccgcgcta tgagcaagtc
     6781 ggtggccatg ttgtcgctcg ccgaaatcat ctcctgcgcc gccttgcgga ctgaaacttg
     6841 cgcaccaggg ggcagttcct cgaggcctgc cgatccgacc gccttggcct cctcggtgat
     6901 ggtcagctga tcggtccagg agacggttcc ggccttgatg gcatccgcaa cagcaagcag
     6961 cacatacagt ttgaagattg aggcgagtgg gagcgacaag tcggtattgc tgcccgccac
     7021 cgtcgagcag ttgccatccg tcaccttgga cacctgatac gagtagcgcg cccccgactt
     7081 cgtcagctcc gcgtcgatgt ctgaccattt cctgatcgcg ggcggtcgca gctccaccgc
     7141 gaaccggtcg accatgttcg cgtggttggt gtgtatttcg atgtcctgtg cggcgccata
     7201 gggcgtcagc gtgtgcaggg tcgcgacccc agcgccgacc tcgatgccgg caacggtgat
     7261 cgggcgatcc acccataggt tgcccatggc agacgtgatg ccgtttaccg cttcgggaat
     7321 ggccatcgtg cgcaccccga ctgttccgat cggccagtcg gagttgacca tgtccatggt
     7381 ctgcttggcc cgcaacccct gaggggtgtt ggtgtcgatg gggacgccat acgccgcatc
     7441 ggccaccggc gctggtccgg tattggtgca gccgcaagcc tgcgcgacga ccagcgcgac
     7501 cgcgagcccg gctgccgtgc gtcgcgcccg gcccgcgcta cgccttggcg ccggaccctg
     7561 caacgtccag cacaacctcg aactcgagca gcgacgcgcc ggtggccacg gggttggcgc
     7621 gctgaccggc gtgtgcctcg atggcaggac cggtcgccca cgcctggaac gcctcctcgg
     7681 actcccactg ggtaacgacg aagtagcggt cttcgccctt gatcgggcgc agcaattgga
     7741 agccgaggaa accgggctgg ttgtcgacgg catgtgcgcg gtgcgcgaac cgcttctcca
     7801 gttccggacc ggcgtcgggc gggacctcga ttgcgttgat cttcaccacg ggcatgcggc
     7861 aaggctaccg tgcggtcacg gcaagataac ggtgtgcacc ctgacctgct cacccgctgc
     7921 ggcggcgacg gtgatccgct ggtgttggtc cacggtctga tggggcgggg cggcacatgg
     7981 acgcggcagg tgccgtggct gacgagactc ggccaggtct acacctacga cgcgccctgg
     8041 cacaggggcc gcgatgtcat cgatccgtat ccggtcagca ccgagcgatt cgtctccgat
     8101 ctcggtgacg cggtcgccga gttgggccgg cccgctgtca tggtcggcca ttcgatgggc
     8161 gcgttgcatt cgtggtgtct tgccgctgac cggcccgacc tcgtcagcgc actggtggtc
     8221 gaggacatgg ccccagactt ccgcggccgc acgaccgggc cgtgggagcc gtggctgcat
     8281 gcacttccgg ccgaattcga ttctgcgcaa aaggtttacg acgagttcgg ccaggtggcg
     8341 gggcaatact tcctggaggc gttcgaccgg actgcgaccg gctggcggct gcacgggcac
     8401 accaggtact ggatcgacat cgccgccgag tggggcaccc gcgactactg gaagcagtgg
     8461 gaggcggtgc gggttcccgc gttgctgctg gaggccggca actccgtcac acccgccggg
     8521 cagatgcgca agatgcagga aaccggctac cggacaacgt atttgcacgt gcccggtgcc
     8581 ggtcacctga tccacgatga acagccgcag atctaccagg aggcggtcga gtcgttccta
     8641 gcggcgctcg ccgacggcac ctgaggcggg ccagaacggc tgcttctcaa agcgggtgcg
     8701 gaccgcgtcg acgtcatggg cgacgcgaaa gccgtcgcgg tcgtcttcga acagcctgcc
     8761 catcatcggt gccgaccagc ggaactggtc gaaccggatg cgcagggagt cattgcgatg
     8821 ggcggcccag ctgagcgccg ctgccaccgc gaacggggcg atcaggatga ggaatgtcag
     8881 tgcagtggtc atggcagtaa tgctccgctg ataaatatcc cgccaacagt ggcaggaagg
     8941 acatagtgcg ataaaatgct gccatggcat taaagagcgt atctgcgctg gtgctggacg
     9001 gtctagcgat ttttgagttc ggcgtgatct gcgaggtctt cggcatcgac cgctccgcgg
     9061 acggtgttcc caacttcgat ttcaaggtct gcggacctga gccgggcaag ccgctgcgga
     9121 catccgtcgg cgccagcatt accccagatc acgggctgga cgacctggtc ggagcggacc
     9181 tcgtcgcgat cccggcaatc agtagccagt acggcggata tctgcccgag gccctcgacg
     9241 ccgtgcgaaa ggcggctgat tcgggctcga tcatcctgac ggtgtgctca ggggcgttcg
     9301 tcgccggtgc cgcgggccta ctcgacggca ggccctgcac cacccactgg atgcacgccg
     9361 acgatctggc ccggatgtat ccgacggcga aggtcgaccg caacgtgctg ttcgtcgacg
     9421 acggaaacct catcaccagc gccggcaccg cggcgggcat cgacgcctgc ctgcacctgg
     9481 tgcgtcgcga gctgggcagc gaggtcacca acaagatcgc acgccggatg gtggttcctc
     9541 cacagcgcga cggcgggcag cgacagtaca tcgatcaacc gattccggtg cgatgttcgg
     9601 aaggctttgc gccgcacctc gattggattc tcaccaacct cgacaggccg catacggtgg
     9661 ccacgctggc cacccgcgcg cacatgtcgg cacgcacttt cgcgcgccgg ttcgtcgagg
     9721 aaaccggccg cacgccgatg cagtgggtga ccgaccagcg ggtgctctac gcgcgacggc
     9781 tgctggagga gagcgatctc gacatcgaca ggatcgccga ccgatccggt ttcgggacgg
     9841 ccacgctgct gcgccaccac ttccgcagga tcatcggcgt gacgccgtcg gattaccggc
     9901 gcaggttctc ctgcagcggt ccgtgcgaag aggccgcagc taccgcttag tcgtcgctcg
     9961 acgggcagga gacgtccacg taggcggtgg tgaacaccgc aggggcgcta ttggacactc
    10021 cgtgcacacc ggtgacgatg cactgggcca gcggaccggt aaccgtcccg ttgatctgga
    10081 cggcgtatcc catgtcctgc agtgtcgtta tggtgtcggc ggccgatccc cctccggtcg
    10141 ggatggcagc ggctgtaggt gccagaccga tcgcgcccgc cgcgaacagg cttgcggtgg
    10201 cggtggcgat tgccaacttg ttcatgactg aactccctct gtctccaaga ggtagcgcga
    10261 ggggttacgc gacagtcagc gcttggcagg ggcgttctcg cggatgagcg tgtgcacccg
    10321 ccagtggcca ggcacgccct tgaggtcgta ctcgccccgt tcggtgaagt ggcgcctcga
    10381 accggtgacg atgtcccgca cggtcgacga cacgagcacc tc
//
LOCUS       DASRJA010000089         6281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3062515,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000089 DASRJA010000000
VERSION     DASRJA010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3062515"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VFC01_04005"
     CDS             complement(<1..1155)
                     /locus_tag="VFC01_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZN78843.1"
                     /translation="MSRIGALQLRDAVLDEGSFRTWDAPPIEVAADYAYRRELADASA
                     KTGLDESVVTGEGTVFGRRVALVACEFDFLAGSIGVAAAERITDAVQRATGERLPLLA
                     SPSSGGTRMQEGTVAFLQMVKIAAAVELHKRAHLPYLVYLRHPTTGGVFASWGSLGHV
                     TAAEPGALVGFLGPRVYEHLYGEPFPAGIQTAENLHRHGVIDGVVPLDALRSTLDRAL
                     KVVADPPGAPPPVPGVEPLPDIEAWASVEASRRPDRPGVRYLLRHGTTERVLLSGTER
                     GEAATMMLALARFGGQPAVVLGQQRVVGGLVGPAALLEARRGMALAAGLRLPLVLIID
                     TAGPALSAEAEQGGLAGEIARCLAELVTLDTPTVSVLLGQGSGGPALAMVP"
     gene            1182..1916
                     /locus_tag="VFC01_04010"
     CDS             1182..1916
                     /locus_tag="VFC01_04010"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZN78844.1"
                     /translation="MIGVTRDGHVMTLEMQRSERRNALNSALVDSLREAVEKAASEDV
                     RAVVLTGQGHVFSSGADLSDPSSVADELPDKAKALNLAIDAAPVPVIGAINGPAIGAG
                     VILSMICDLRVVAPDAYFQFPVAKYGLALDNWSIRRLTSLVGAGRARGMLLAAERLTA
                     DVALQTGMANRIGTLADAQAWAAEIAGFAPLALKHAKRVLNDDGAYEDPWPEHQDLFD
                     KAWSSQDVIEAQVARIEKRPPRFQGA"
     gene            1919..3031
                     /locus_tag="VFC01_04015"
     CDS             1919..3031
                     /locus_tag="VFC01_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN78845.1"
                     /translation="MARAALRFGFGTASLLAGGWLLRATRGAPAALGASPAEIQAVAR
                     RSPNFQDGVFVNVDPASTVSLDREQQRLLLRELVGSRDHARPPGPIPLATPASTDAVA
                     ANLAVSWYGHSTAMIEVDGYRVLADPVWSRRVSPSHTVGPQRMHEVPVPLEALPAVDA
                     VLISHDHYDHLDIDTTIGLARTQRAPFVVPLGVGAHLRKWGIPEARIVELDWNESHRI
                     GELTLVCTPARHFSGRLFSRNTTLWASWVIAGPRHRAFFGGDTGYTKTFAEIGMDHGP
                     FDVTLLPIGAYHPAWPDIHMNPEEAVRAHLDVAEADSGLLVPIHWATFRLAPHPWAEP
                     VERLLRAADPAGVHVVVPKPGQRVDPDTSVDPWWQL"
     gene            3071..4648
                     /locus_tag="VFC01_04020"
     CDS             3071..4648
                     /locus_tag="VFC01_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZN78846.1"
                     /translation="MNALARTAAAALLLTLVAGCNTKPAEPAPPPTAQSQSDLPPPLV
                     PAMPLPDNAVENAVAKLDGIANDLMKKSGIPGMAVAVVHGGKTVYAKGFGVKDVRSGD
                     KIDPDTVFQLASVSKSLSATVVAQQVGTNAIGWDTPIVSKLPWFALSDPVVTPMVNVG
                     DMFSHRSGLPDHAGDLLEDLGYDRRYVLERLRQLPLEPFRISYAYTNFGLTAGAEAVA
                     VSAGKSWDDLADEVLFRPLGMASASYRFTDYEARPNRAIGHIHVDGRYEPLYVRDADP
                     EGPAGGASSSVNDMTRWLAMVLADGGHDGKQIVDSRALLPAVTPQMVSSPATEPAMRS
                     GFYGYGFNVGATSAARMELSHSGGFVLGAGTNFVILPSADVAIVALTNATVSGVPEAL
                     TAEFADLVQFGEVREDWYKLYGDIFKQMEQPTGSLVGQKPPASPAPAAPLASYVGTYH
                     NDYWGPATITEKDGKLHLALGTKLDVPLDHWDGNVFTYAWVSENSPPGTVSKATFDGN
                     KLALEFYDTFNKGTFTK"
     gene            4645..>6281
                     /locus_tag="VFC01_04025"
     CDS             4645..>6281
                     /locus_tag="VFC01_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZN78847.1"
                     /translation="MTTIPGVEATGLTDAEVAQRVADGKTNDVPTRAARSVSDIVRAN
                     VFTRINAILGVLLIIVLSTGSIINGAFGLLIIANSAIGIIQELRAKQTLDKLAIVGQT
                     KPLVRRQSGTQALLPSEVVLDDIIELGPGDQIVVDGEIVEETNLEVDESLLTGEADPI
                     AKDASENVMSGSFVVAGSGAYRATKVGREAYAAKLADEASKFTLVNSELRSGINKILQ
                     FITYLLVPAGLLIIYTQLFTTDAGWRESVLRMVGALVPMVPEGLVLMTSIAFAVGVVR
                     LGRRQCLVNELPAIEGLARVDVVCADKTGTLTENGMRVSDLKPLSEAAVGDILAQLAS
                     DDARPNASMAAIGEAYKMPPGWTATATAPFKSATKWSGASYGEHGNWVIGAPDVLLEP
                     GSEAAAQAEQIGSLGLRVLLLGSCDLPVDHPDAPGTVTPVALVVLEQRVRPDARDTLD
                     YFASQKVTIKVISGDNAVSVGAVAGSLGLEGETMDARQLPDQPEQLADTLEEYTTFGR
                     VRPDQKRAMVHALQSRGHTVAMTGDGVNDVLALKDAD"
BASE COUNT         1052 a         2131 c         2132 g          966 t
ORIGIN      
        1 gggcaccatc gccagcgctg gcccgccgct gccctgcccc aacaacaccg acaccgtggg
       61 cgtatccagc gtgacgagtt cggcaaggca gcgggcgatc tcaccggcca acccaccctg
      121 ttcggcctcg gccgacagcg cggggcccgc ggtgtcgatg atgagcacca gcggcaggcg
      181 caaacccgcc gccaacgcca taccgcgccg ggcctccaac agcgccgccg gaccgaccaa
      241 cccaccgacg acacgctgct ggccgaggac gacggcgggc tgaccgccga agcgcgccag
      301 cgccaacatc attgtggccg cctcgccacg ctcggtgccc gacagcagta cgcgctcagt
      361 tgtgccgtgg cgcaacagat atcggacgcc gggacggtcc gggcgccgcg acgcctctac
      421 cgaagcccac gcctcgatgt cgggtagtgg ctcgaccccg ggaacgggtg gcggcgcccc
      481 tggcggatcg gcgacgacct tgagcgcgcg gtccagcgtc gagcgcaatg cgtcgagcgg
      541 gacgacgccg tcgatgacgc cgtgccggtg caggttctcc gcggtctgga tgccggccgg
      601 gaacggctcg ccgtacagat gctcgtagac ccgcggcccg aggaagccga cgagcgcgcc
      661 gggttccgcc gcggtgacgt gaccgagcga cccccacgac gcgaacaccc cgccggtggt
      721 ggggtggcgc aggtacacga ggtagggcag atgcgcgcgc ttgtgtagct cgacggccgc
      781 cgcgatcttg accatctgca ggaacgcgac ggtgccctcc tgcatgcggg tgccgccgga
      841 gctcggtgac gccagtagcg gcagccgctc gcccgtcgcc cgctgcactg cgtcggtgat
      901 gcgctcggcg gcggccaccc cgatcgagcc cgcgaggaaa tcgaactcgc acgcgaccag
      961 ggcgacgcgg cggccgaaga cggtcccctc gccggtcacc accgattcgt ccaagccggt
     1021 cttcgcggag gcgtcagcca gctcgcgtcg gtacgcgtaa tcagcggcga cctcgatcgg
     1081 cggggcgtcc caggtgcgga acgagccctc gtccagcacg gcatcgcgca gctgcagggc
     1141 accgatccgg ctcacatgac gaccctatat aggctggccg catgatcgga gtgacccgcg
     1201 acggccacgt gatgaccctg gagatgcagc gctccgagcg ccgcaacgca ctgaacagcg
     1261 cgctcgtcga cagcctgcgc gaggcggtcg agaaggccgc gtccgaagat gtccgggccg
     1321 tcgtgctgac cggccagggc cacgtgttca gctcgggcgc cgatctgtcc gacccctcga
     1381 gtgtggccga cgagctgccc gacaaggcca aggcgctgaa cctcgccatc gacgcagcgc
     1441 cggtcccggt catcggcgcg atcaatggac ccgcgatcgg cgccggtgtc attctctcga
     1501 tgatctgcga tcttcgggtc gtcgccccgg acgcctattt ccagttcccg gtcgcgaaat
     1561 atggtctggc gctggataac tggagcattc gtcggctgac gtcgttggtg ggagccggcc
     1621 gggccagggg catgctgttg gcagcggaac ggctcaccgc cgacgtcgcg ctgcagaccg
     1681 ggatggccaa ccgcatcgga acattggccg acgcgcaggc ctgggccgca gagatcgccg
     1741 ggttcgcgcc gctcgccttg aagcacgcca agcgggtgct caatgacgac ggcgcctacg
     1801 aagacccgtg gcccgagcac caggacttgt tcgacaaggc atggtccagc caggacgtca
     1861 tcgaggcaca ggtggcgcgc atcgagaagc gaccgccgag attccagggg gcttgatggt
     1921 ggcgcgcgca gccctgcgct tcgggttcgg cacggcatcc ttgctggccg gcgggtggct
     1981 gctgcgcgcg acgaggggcg cgcccgcggc gctgggtgcc tcacccgccg agatccaggc
     2041 tgtggcgcgg cgctcgccga acttccagga cggcgttttc gtcaacgttg acccggcgtc
     2101 gacggtcagc ctcgaccgcg aacagcagcg gctgctgctg cgcgagcttg tcgggtctcg
     2161 cgatcatgcc cggccacccg ggccgatacc gctggcgacg cccgcgtcca ccgacgccgt
     2221 agctgcgaac ctcgcggtca gttggtatgg gcactccacg gcgatgatcg aagtcgacgg
     2281 ctaccgcgtg ctcgccgacc cggtgtggag tcgcagggtc tcgccgtcgc acacggttgg
     2341 cccacagcgc atgcacgagg tacccgtacc gctggaagcg ttacccgccg tcgacgcggt
     2401 gctgatcagc cacgaccact acgaccacct cgacatcgac acgaccatcg ggctggcccg
     2461 cacgcagcga gcgccgttcg tcgtaccgct cggcgtcggc gcgcacctgc gcaaatgggg
     2521 catcccggag gcgcgcatcg ttgagctcga ctggaacgag agccaccgga tcggcgagct
     2581 gaccctggtg tgcacgcccg cccggcactt ctccgggcgg ctgttctccc gcaacaccac
     2641 gctgtgggcc tcatgggtga tcgccggtcc gcggcaccgc gcgttcttcg gcggcgacac
     2701 cggctacacc aagaccttcg ccgaaatcgg catggaccac gggccattcg acgtgacgct
     2761 gctgccgatc ggcgcgtatc acccggcgtg gccggatatt cacatgaacc cggaggaggc
     2821 cgtccgcgcc cacctcgacg tcgccgaggc cgattcggga ttgctggtac ccattcactg
     2881 ggccacgttc cggctggcgc cgcacccgtg ggcggagccg gtggagcggc tgctgcgggc
     2941 cgctgacccg gcaggcgtgc acgtggtggt gcccaaaccg gggcagcggg tcgatcccga
     3001 cacgtcggtt gatccgtggt ggcagctgta atacctcgcc tgtgcgcggt tagccgctac
     3061 cgttcacgtc atgaatgcac tggccaggac tgccgctgcc gcgctgctcc tgacattggt
     3121 ggcgggctgt aacaccaagc cggctgaacc ggcgccgccg ccgaccgccc agtcgcagtc
     3181 cgatctgccg ccgccgctgg tgccggcaat gccgttgcct gacaacgccg tggagaacgc
     3241 ggtggccaag ctcgacggga tcgccaacga cttgatgaag aagtcgggca tcccgggaat
     3301 ggctgtcgcc gtggtgcatg gcgggaagac cgtgtacgcc aagggcttcg gggtcaagga
     3361 cgtacgcagc ggtgacaaga tcgacccgga caccgtgttc cagctggcat cggtgtccaa
     3421 gtcgctgagt gcaaccgtgg tcgcccaaca ggtgggcacc aacgcgatcg gctgggatac
     3481 accgatcgtg tccaagctgc cgtggttcgc gctgtcggat ccggttgtca cgccgatggt
     3541 caacgtgggg gacatgttct cgcaccggtc tgggctgccc gaccacgcag gcgacttgct
     3601 ggaggacctc ggctacgacc ggcggtacgt gctcgaacga ctccggcagc tgccactcga
     3661 gccgttccga atctcgtatg cctacaccaa ctttgggttg accgcgggtg ccgaggcggt
     3721 cgcggtgagc gctggtaagt cgtgggacga cctcgccgac gaagtgctgt tccgtccgct
     3781 cgggatggca tcggcgagct accggttcac cgattacgaa gcccggccca accgcgccat
     3841 cggccatatc cacgtcgatg ggcgctacga accgctctac gttcgcgacg ccgatcccga
     3901 agggcccgct gggggggcga gctcctcggt gaacgacatg acgcgctggc tggccatggt
     3961 gctggccgac ggcggccacg acggcaagca gatcgtcgac tcacgggcgt tgctgcctgc
     4021 ggtgacgcca cagatggtgt cgagccccgc gacggagccc gcgatgcggt cggggttcta
     4081 cggttacggg ttcaacgtgg gtgcgacgtc ggcggcgcgc atggagctca gccactccgg
     4141 cgggttcgtg ctcggcgcag gcaccaattt cgtcatcctg ccgtcggctg acgtggcgat
     4201 cgtcgcgctc accaacgcca cggtgtccgg tgtgcccgaa gcgttgacgg ccgaattcgc
     4261 cgacctcgtg cagttcggcg aggtgcgcga ggactggtac aagctctacg gcgacatctt
     4321 caaacagatg gagcagccga cgggctcgct ggtcggacag aagcccccgg cgagccccgc
     4381 gcccgccgcg cccctcgcgt cctacgtcgg cacataccac aacgactact ggggcccggc
     4441 cacgatcacg gagaaagacg gcaagttgca cctcgctctc ggcacgaaac tggatgtgcc
     4501 cctggaccat tgggatggca acgtcttcac ctatgcgtgg gtctcggaaa actccccgcc
     4561 gggaacggtt tccaaggcga cgtttgacgg caacaagctc gccctggagt tctacgacac
     4621 gttcaataag gggacgttca cgaaatgacg accatccccg gcgttgaggc cacgggcctg
     4681 accgatgccg aggtcgccca gcgggtagcc gacggcaaga ccaacgacgt gcccacccgt
     4741 gcggcgcgca gcgtgtccga catcgtcagg gccaacgtct tcacccgcat caacgccatt
     4801 ctgggtgtgc tgctgatcat cgtgctgtcc accggctcga tcatcaatgg tgcgttcggc
     4861 ctgctgatca tcgccaacag tgctatcggc atcatccagg agctgcgtgc caagcagacg
     4921 ctcgacaagc tggccattgt cggacagaca aaaccgttgg tgcgcaggca atccggaacc
     4981 caggcgcttc tgcccagcga ggtcgtgctc gacgacatca tcgaactcgg gccgggcgat
     5041 cagatcgtgg tggacggtga gatcgtcgag gaaaccaatc tcgaggtcga cgaatcacta
     5101 ctcaccggtg aggccgaccc gatcgcgaaa gacgcaagcg aaaacgtgat gtcgggcagc
     5161 ttcgtggtcg caggcagcgg ggcgtaccgg gccaccaagg tcggccgcga ggcctatgcc
     5221 gccaagctgg ccgacgaagc cagcaagttc acgctggtga actccgaact gcgcagcggc
     5281 attaacaaga tcctgcagtt catcacgtac ctacttgtcc ccgccggcct gctgatcatc
     5341 tacacccagc tgttcaccac cgacgcggga tggcgtgaat cggtgctgcg gatggtcggt
     5401 gctttggtcc cgatggtgcc cgaaggcctg gtgctgatga cctcgatcgc gttcgccgtc
     5461 ggggtggtgc ggctcggtcg caggcaatgc ctcgtcaacg agttaccggc gatcgagggg
     5521 ctcgcccgtg tcgacgtggt ttgtgccgac aagacaggca ctttgaccga aaacggtatg
     5581 cgggtaagcg atctcaagcc gctgtcggaa gccgccgtcg gcgacatcct ggctcagctc
     5641 gcgtccgacg acgcccggcc gaacgccagc atggctgcca tcggcgaggc ctacaagatg
     5701 ccaccggggt ggaccgccac cgcgaccgcg ccgttcaagt cggcgaccaa atggagcggc
     5761 gcgtcctacg gcgagcacgg caactgggtg atcggggcgc ccgacgtgct gctcgagccg
     5821 gggtccgagg ccgccgcgca ggccgagcag atcgggtcgc tgggcctgcg ggtgctgctg
     5881 ctcggctcgt gcgacctgcc cgtcgaccat cccgacgcgc caggcaccgt cacaccggtg
     5941 gcgctggtgg tgctcgagca gcgcgtccgc ccagacgccc gcgacacgct ggattacttt
     6001 gcctctcaga aggttacgat caaggtgatc tccggcgaca atgcggtgtc ggtgggtgcc
     6061 gtggccggct ccctcggtct ggagggcgag acgatggatg cccgtcagct gcccgatcaa
     6121 ccggagcagc tggccgacac acttgaggag tacacgacgt tcggccgggt gcgccccgac
     6181 cagaagcgtg cgatggtgca tgcgctgcag tcacgcgggc acacggtcgc gatgaccggt
     6241 gacggcgtca acgatgtgtt ggcgctcaag gacgccgaca t
//
LOCUS       DASRJA010000090         3760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1326092,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000090 DASRJA010000000
VERSION     DASRJA010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326092"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFC01_04030"
     CDS             complement(<1..375)
                     /locus_tag="VFC01_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN78848.1"
                     /translation="MEAVGGLFAMSKDAVRFIFRRPFQWREFLEQSWFVVKVSLAPTL
                     LVAIPFTVLVSFTLNILLRELGAADLSGAGAAFGAVTQVGPLVTVLIVAGAGATAMCA
                     DLGSRTIREEIDAMEVLGIDPVQ"
     gene            complement(428..1597)
                     /locus_tag="VFC01_04035"
     CDS             complement(428..1597)
                     /locus_tag="VFC01_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZN78849.1"
                     /translation="MRKVAIVGAGMTAFGEHFALGIKDLLPMAFADCAKSVDKGLAKS
                     DIQGAWFGAMGTADGFPSGILADSLGLPDLPVTRVENSCATGNDAVRNALFGVASGAF
                     DVALVMGADKLRDTTSKDMLWKWEAMARDMAWDYPLGLVSPAGFALHVRRYMHESPTT
                     REHLAMVAVKNHRHGVNNPKARLRFEITLEQALEAPTIVTPFGLYDCAPQSDGAAALV
                     IAAEDVVERFTDRPVWIRGVGLGLDSVMHQHKPDMTTFPATTRAAKQAFAMAGMTPAD
                     VDVSEVHDFFTGIELISYEDLGFAERFGGYKLVEAEVTSVGGALPVNPSGGLKAKGHP
                     PGATGVAQCVELFAQLRGEAVNQVDGARVGLAHNIGGPTAVAAVTLLEGSAHGAR"
     gene            complement(1594..2766)
                     /locus_tag="VFC01_04040"
     CDS             complement(1594..2766)
                     /locus_tag="VFC01_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZN78850.1"
                     /translation="MPYVASIGTYLPCWGAPQHRVPGDDEDAITLAVEAGRAALIASG
                     AVERVVLVSRDMPLLESSNAAVLLAGLGLDPELEVDERLGGAPATLDAVSSARPRTLI
                     IGTDLDPAGAAAILTAERGLQVRTAARVARSLPVRTRNATGDVHDYGDPRLLHERGLV
                     ASLAAAWLDTPSAVAGVEHERAVELCIGAPPPLPTTGASASLFALAGMADAGTTGPLV
                     AVEQASLSGVTVSGGVAELHRREPPARPAPAGTYLEGADIPISLAAYERAFEAKTRWE
                     AGRFAASDELDFPPRYRLAEDGALASDYELIPLPRTGTVYTETTVHMPVPGLRTPYSL
                     VIVELDGVGVRALVKVTGAEAGTVDIGARGRLALRRVAVRSGVPDYGYMFEPERAA"
     gene            complement(2784..>3760)
                     /locus_tag="VFC01_04045"
     CDS             complement(2784..>3760)
                     /locus_tag="VFC01_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN78851.1"
                     /translation="SVPEGLEVLLNLARRADVFLTSFLPGHRQKFGIDVDDIRAVNPN
                     IIYARGSALGPRGEESVKGGYDMTAFWCRAGTAATITPPGIEGMIGPPGPAYGDTISG
                     TNLAGGIAAALLKRERTGEPSVVDVSLLGSGLWSMGHTVALTSHLNQLMVQPPPGVHG
                     SPINPLVGVYPTADDRYISFVMMQPTKFWADVCRHMDLEDLIDDPRFATVESIAENTA
                     AAVEILREAMTKRTLPEWSERFSTLAGPWAPVQDTLQAAEDAQIRANEYIVSTGELEL
                     VANPVQFDVSAPQTGPAPGFAEQTDEILLELGLDWDRIIELKTAGAVT"
BASE COUNT          623 a         1320 c         1204 g          613 t
ORIGIN      
        1 ttgcaccgga tctatgccca gcacttccat cgcgtcgatt tcctcgcgga tggtgcgtga
       61 ccccagatcc gcgcacatcg ccgtggcacc cgcgcccgcc acgatcagca ccgtcaccag
      121 gggaccgacc tgggtcaccg cgccgaacgc tgcaccggca ccgctcaagt cggcggcgcc
      181 gagttctcgc aacaaaatgt tgagtgtaaa gctgaccaac accgtaaagg ggatcgccac
      241 cagcagtgtg ggcgccagcg ataccttcac cacgaaccac gactgctcca gaaattcccg
      301 ccactgaaac ggtcggcgga agatgaagcg cactgcgtcc ttcgacatcg cgaacagccc
      361 gccgacagcc tccatcggct tggaaccacg gcccaacgag atccctggcg accaccgttc
      421 cgccccgcta ccgcgcgcca tgagcggagc cttccaggag ggtgacggca gccaccgccg
      481 ttgggccgcc gatattgtga gccaatccga cccgcgcgcc gtccacttgg ttgactgcct
      541 cccctcggag ttgcgcgaat agctccacgc actgtgcgac acccgtcgcc cccggaggat
      601 ggcccttggc cttcagcccc ccgctggggt tgaccggcag cgcaccgccg acacttgtca
      661 cctcagcctc gacaagcttg tagcccccga accgctcggc aaagcccaaa tcttcatagc
      721 tgatcagctc aattcccgtg aagaaatcgt gaacttcgct gacgtcgacg tcagccggtg
      781 tcatgccggc catcgcaaat gcctgcttgg cggcgcgcgt ggtggccgga aacgtcgtca
      841 tatccggctt gtgttgatgc atcaccgagt cgagcccaag gcctacgccg cggatccaca
      901 ccggacggtc ggtgaatctc tcgacaacat cctctgcggc gatgacgagc gcggcggcgc
      961 cgtcgctctg cggggcacag tcgtagagcc cgaacggagt cacgatggtg ggcgcctcga
     1021 gcgcctgttc cagcgtgatc tcgaagcgca accgcgcctt cgggttgttc actccgtggc
     1081 ggtggttttt gaccgcgacc atggccaagt gttcgcgcgt cgtcggggac tcgtgcatgt
     1141 agcggcggac gtgcagcgcg aatcctgccg gcgagaccaa cccgagcggg tagtcccacg
     1201 ccatgtcgcg cgccatcgcc tcccacttcc acagcatgtc cttggacgtg gtgtcgcgca
     1261 gcttgtccgc gcccatcacc agggccacgt cgaatgcgcc tgacgcgacg ccgaacagtg
     1321 cgttacgcac cgcgtcattg cctgtggcac aagagttttc gacgcgcgtg acgggcaggt
     1381 caggaagtcc gagcgagtcg gccaggatgc ccgacgggaa gccgtcggcc gttcccatcg
     1441 cgccgaacca tgcaccttgg atgtcggact tcgccaagcc cttgtcgacg cttttcgcgc
     1501 agtccgcgaa cgccatcggt agcaggtcct tgatgccgag cgcgaaatgc tcgccaaacg
     1561 ccgtcatccc ggccccgaca atcgccacct tcctcatgcg gcccgctccg gctcgaacat
     1621 atagccgtag tccggcacgc ccgaacgcac cgcgacacgg cgcagcgcca gtcgtccgcg
     1681 ggcgccgatg tcgacggtgc ccgcctcggc gccagtgacc ttgaccagtg cgcgcacccc
     1741 gacgccgtcg agttcgacga tcaccagaga gtacggcgtg cgcaagcctg gcaccggcat
     1801 gtgcacggtg gtttcggtgt agaccgtccc ggtacgcggc agcgggatca attcatagtc
     1861 ggatgcgagc gccccgtcct cggctagccg gtagcgcggc gggaaatcca attcgtcaga
     1921 agcggcgaat ctgcctgcct cccaacgggt cttggcctcg aaggcccgct cataggcggc
     1981 cagtgagatc gggatgtccg cgccttcgag ataggttccg gcgggcgcag gccgcgcagg
     2041 cggctcgcgc cggtgaagct ccgcgacgcc gccgctgacc gtcacaccgg agaggctggc
     2101 ctgttcgacg gccaccaatg ggcccgtcgt tcccgcgtcg gccatgcccg caagcgcgaa
     2161 gagactggcg ctggcgccgg tggtcggcaa gggcggtggc gcaccgatac acaactcgac
     2221 ggctcgttcg tgctcgacgc ctgccaccgc ggacggggtg tcgagccatg cggcggccag
     2281 cgacgcgacc aggccgcgct cgtgcagcag ccgggggtcg ccgtagtcgt gcacgtcgcc
     2341 ggtcgcgttg cgggtgcgca ccggcaggct gcgcgcaaca cgcgccgcgg tacgaacctg
     2401 cagtccccgc tctgcggtca ggatggccgc cgcgcccgcc gggtccagat cggtgccgat
     2461 gatgagtgtc cgcggccggg ccgaactgac cgcgtcgagt gtcgcgggcg cgccgccgag
     2521 gcgttcgtcg acctcgagtt cggggtcgag tcccagtccc gccagcagca ccgcggcgtt
     2581 gctgctctcc agcaacggca tgtcgcggct gaccaagacg actcgctcga ccgccccgct
     2641 ggcaatcaac gcggcgcgcc cggcctcgac cgcgagggtg atcgcgtcct cgtcgtcacc
     2701 gggcacccgg tgctgaggcg cgccccagca cggcaggtag gtgccgatcg acgcgacata
     2761 gggcattgag atctctcccg aacctaggtg acggcgccgg ccgtcttgag ctcgatgatg
     2821 cggtcccaat ccaaaccgag ttccagcaag atttcgtcag tttgctccgc aaagccaggg
     2881 gcgggtccgg tttgcggtgc gctgacatcg aactgcaccg gattcgcgac caattcgagt
     2941 tcacccgtcg agacgatgta ctcgttggcc cggatctggg cgtcctcagc ggcttgcagg
     3001 gtgtcctgca cgggcgccca cggcccggcc aatgtcgaga accgctcgct ccattcgggc
     3061 agcgtccgct tggtcatcgc ctcccgcagg atctcgacgg ccgctgcggt gttctcggcg
     3121 atcgactcga ccgttgcgaa ccgcggatcg tcgatcagat cctcgaggtc catgtgcctg
     3181 cacacgtcag cccagaactt ggtcggctgc atcatcacga acgagatgta gcggtcatcg
     3241 gcggtcggat acacgccgac gagcgggttg atcggtgagc cgtgcacccc tggcggcggc
     3301 tgcaccatca gctgattcag gtgtgacgtc agcgcgacgg tgtggcccat cgaccacagg
     3361 ccgctgccca acagcgacac gtcgaccacc gacggttcgc cggtgcgctc gcgcttcagc
     3421 agcgcggccg cgattccgcc tgccaggttg gtgccggaaa tcgtgtcgcc gtaggccggt
     3481 ccgggcgggc cgatcatgcc ctcgatgccg ggcggggtga tggtcgccgc cgtgcccgcc
     3541 cggcaccaga acgcggtcat gtcgtagcca cccttgaccg actcctcacc acgcgggccc
     3601 agcgcgctgc ccctggcgta gatgatgttc ggattcaccg cgcggatgtc atcgacatcg
     3661 atgccgaact tctgccgatg tcctggcagg aagctggtga ggaacacgtc ggcgcggcgc
     3721 gcgaggttga gcagcacctc cagtccttcg ggtaccgaca
//
LOCUS       DASRJA010000091         9266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1614222,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000091 DASRJA010000000
VERSION     DASRJA010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614222"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VFC01_04050"
     CDS             complement(<1..886)
                     /locus_tag="VFC01_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZN78852.1"
                     /translation="MTSHDTVYLDHAATTPMHPAAIEAMTAVLATVGNASSLHGTGRV
                     ARRRMEEARESLAHLLGARPSEVIFTAGGTESDNLAIKGIYWARRDADPRRRRIVTTP
                     VEHHAVLDSVQWLAEHEGAEVTWLPVGDDGSVSPAALRGALQAHDDVALVTIMWANNE
                     VGTIMPITELATIAAEFDVPMHSDAVQAIGQIPVDFAASGLSAMSIAAHKFGGPTGVG
                     ALLLRRDTSCVPLLHGGGQERDVRSGTPDVAGAVAMAAAAKVAIENLTANSTRVQALR
                     DRLIDGVLSTIDDVYLNGA"
     gene            complement(935..1765)
                     /locus_tag="VFC01_04055"
     CDS             complement(935..1765)
                     /locus_tag="VFC01_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN78853.1"
                     /translation="MTIEHAWLPRATCDATCVSADAVAPGQPVVVAWRIAMRATFALI
                     LLSGSPLLAVPMPGRSHIQRGYCRMMLRCLGVRITVSGGPIRNLRGVLVVSGHVSWVD
                     IFAIGAVMPGRFVARADLVDWIGLGMVARLMKVIPIDRASLRRLPDVVGTVAARLRAG
                     QTIVAFPEGTTWCGLAYGPFRPAMFQAAVDAGRPVQPLRLSYHHRDGRPSTVAAYVGD
                     DTLMASIRRLVTARRTVVHVQVESLQLPGTSRRDLAARCEAAVRGDATLRGHGHALVA
                     "
     gene            complement(1762..2592)
                     /locus_tag="VFC01_04060"
     CDS             complement(1762..2592)
                     /locus_tag="VFC01_04060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HZN78854.1"
                     /translation="MSTASVLIAADPTTPSADTPRYTLLLSTDPALIDAAQRLRHDVF
                     TSEPGFALAGATDGRDADRFDEHCDHLLVREDNSGELVGCYRMLPPPGAIAAGGLYTA
                     TEFDVRELDSLRPSLVEMGRAVVREDHRNGGVVLLMWAGILAYLDRCGYDYVAGCVSV
                     PVDSADGEAPGRQIRGVRDFVRRRHAAPYTVHPYRPVTIGGKGLDDIDPPARVSVPPL
                     MRGYLRLGAKVCGDPAHDPDFGVGDFPALLDKRHADVRYLRRLRSVGAAAAMADGMSS
                     "
     gene            complement(2684..3337)
                     /locus_tag="VFC01_04065"
     CDS             complement(2684..3337)
                     /locus_tag="VFC01_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HZN78855.1"
                     /translation="MTELGEVVRDRRSTRLFLRDKPVPRELLDEALQLAMRAPSNSNV
                     QPWRVFIASGPRRDRLVEALLEEASVELPVTTGLPEHFIPLRQELGALVYGAMGIARH
                     DAEARLLAQLRNWEFFRAPVGAVVCMHRDLGLVDALGVGMFLQTLVLALTERGLGSCV
                     QVSIAAYPEILRAHLDIPDDLTVLCGLAIGYPDPAFPGNSLAVPRNQVDKNVVFLDS"
     gene            complement(3334..3738)
                     /locus_tag="VFC01_04070"
     CDS             complement(3334..3738)
                     /locus_tag="VFC01_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN78856.1"
                     /translation="MFDAADLAKAVSTIQTVTPPFIPSDAEVMTIVIEWPAGSAGAPP
                     HRHPGGPSFGYVLEGEMLFELEGEAPRVIKAGEAFWEPGGDAIHYSDANNRSDIPLRY
                     VVTMVCTPGAPMLVFVDEDELEVRKDRRVPRR"
     gene            complement(3767..4459)
                     /locus_tag="VFC01_04075"
     CDS             complement(3767..4459)
                     /locus_tag="VFC01_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005056740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZN78857.1"
                     /translation="MKISVIGATGLIGSKVVDLLKGDGQDVVAASRASGADVLTGEGL
                     ADALAGADVLVDLTNSPSFDDDPVMDFFTRSSANLVAAAKESGVGHYVALSIVGVDGL
                     PESGYMRAKVVQEKTIAESGLLYTIVRATQFQEFAAAIVGSLDAGDEVRVPDALIQPI
                     AADDVAAEVARAAVAGPADSVVNIGGPEKISFADLARSVLAKQGVDKTVVVDPQATYF
                     GTRLQQHSLVTA"
     gene            complement(4586..5542)
                     /locus_tag="VFC01_04080"
     CDS             complement(4586..5542)
                     /locus_tag="VFC01_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZN78858.1"
                     /translation="MAEVLVLVEHADGAVKKITAELITAARKLGEPAAVVVGKPGTAA
                     PLTDALKEAGAAKIYVAESDDAENYLITPYVDVLAALTESAAPAGVLLAASADGKEIG
                     GRLAARTGAGLLVDVVEVKDGGKAVHSIFGGAFTVEAEATGDTPVITVRPGAIEAEPA
                     AGAGEVVNVEVPAQAENATKIIKREPAVAGDRPELTEATVVVSGGRGVGSAENFSVVE
                     ELADSLGAAVGASRAAVDSGYYPGQFQVGQTGKTVSPQLYIALGISGAIQHRAGMQTS
                     KTIIAVNKDEEAPIFEIADLGIVGDLFKVAPQLTEAVKARKG"
     gene            complement(5579..6379)
                     /locus_tag="VFC01_04085"
     CDS             complement(5579..6379)
                     /locus_tag="VFC01_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZN78859.1"
                     /translation="MTNIVVLIKQVPDTWSERKLTDGDFTLDREAADAVLDEINERAV
                     EEALLIKEKEAANGTESTVTVLTAGPARATEAIRKALSMGADKAVHLLDDGLHGSDLI
                     QTGWALARALGTIEGTELVIAGNEATDGVGGAVPAVIAEYLGLPQLTHLRKVSVEGGK
                     VTGERETDDGVFTVEAPLPAVVSVNEKINEPRFPSFKGIMAAKKKEVTTLTLAEIGVE
                     ADEVGVANAGSKVTASTPKPPKTAGEKITDEGDGGTKVAEYLVAQKII"
     gene            6575..7354
                     /locus_tag="VFC01_04090"
     CDS             6575..7354
                     /locus_tag="VFC01_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN78860.1"
                     /translation="MSASFVTNGPLGDDALPLTGERTIPGLAEENYWFRRHEVVYERL
                     AGRCADRDVLEAGCGEGYGADLIADVARRVIGLDYDESAVAHVRARYPRVEMLHGNLA
                     ELPLPDAAVDVVVNFQVIEHLWDQGQFVAECFRVLRPGGALLISTPNRITFSPGRDTP
                     INPFHTRELNAAELTELLTTAGFAVEAMLGVFHGSRLAELDERHGGSIIDAQIARALA
                     DAPWPEDLLADVASVATDDFDVVDDRDIDDSLDLVAIAVRP"
     gene            7351..8874
                     /locus_tag="VFC01_04095"
     CDS             7351..8874
                     /locus_tag="VFC01_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HZN78861.1"
                     /translation="MTNSERVPGLFTLVLHTHLPWLAHHGRWPVGEEWLYQSWSAAYL
                     PLMRVLRTLADEGRTRVVTLGMTPVVTAQLDDPYCLSGMHHWLANWQLRALEATTLGD
                     PLREFGIREHTEAGHALEEYATLWRHGGSPLLRVLIDAGTIELLGGPLSHPFQPLLNP
                     RLREFALREGLADAGQRFAHTPRGIWAPECAYAPGMEIDYAAAGVGHFMVDGPSLHGD
                     TALGRPVASSDVVAFGRDLQVSYRVWSPKSGYPGHAAYRDFHTYDHVTGLKPARVTGR
                     NIPSDAKAPYDPQRADRAVDVHVADFVGVVRDRLISESERIGRPAHVVAAFDTELFGH
                     WWYEGPVWLERVLRALPEAGVRVGTLSDAVADGFVGAPVELPPSSWGSGKDWQVWAGE
                     KVADLVQLNTEVVDTALTTVDKALGETDAPPARNFVADQILRETLLTVSSDWPFMVSK
                     DSAADYARYRAHLHAHATREIAGALASGRNEHAQRLADNWNRADGLFGALDARRLPR"
     gene            8871..>9266
                     /locus_tag="VFC01_04100"
     CDS             8871..>9266
                     /locus_tag="VFC01_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HZN78862.1"
                     /translation="MKVLIVSWEYPPVVIGGLGRHVYNLATALAAAGHEVVVLNRRPG
                     GTDPSTHPSTDEIAEGVRVVAAAQDPHEFDFGADMMAWTLAMGHSMVRAGLGIRDRRG
                     RPWRPDVVHAHDWLVAHPAIALAEYLDVPL"
BASE COUNT         1416 a         3142 c         3164 g         1544 t
ORIGIN      
        1 tggcgccgtt gaggtagacg tcatcgatcg tggacagcac gccgtcgatg agccggtcgc
       61 gcaacgcctg tactcgggtg ctgttggccg tcaggttctc gatggccacc tttgctgcgg
      121 cggccattgc caccgcaccc gcgacatccg gtgtgccaga acggacgtcg cgctcctggc
      181 caccgccgtg cagtagcggc acacagctgg tgtcgcggcg tagcagcagc gcaccgacgc
      241 cggtcgggcc gccgaatttg tgcgcggcga tgctcatcgc cgacaggccg ctcgccgcga
      301 agtccaccgg tatctgcccg atcgcctgga cggcatcgct gtgcatcggc acgtcgaatt
      361 ccgcagcgat ggttgccaat tcggtgatcg gcatgatggt gccgacctcg ttgttggccc
      421 acatgatcgt caccaacgcg acgtcgtcgt gagcctgcaa tgcccctcga agcgcggcgg
      481 gtgagaccga gccgtcgtcg ccgaccggca gccaggtgac ctcggcacct tcgtgttcgg
      541 cgagccactg caccgagtcg aggacggcat ggtgctcgac cggggtggtc acgatccggc
      601 gacgacgcgg gtcggcatcg cggcgcgccc agtagatgcc cttgatcgcg aggttgtcgc
      661 tctcggtgcc gcctgccgtg aagatcacct ccgacggtcg cgcgccgagc aggtgggcca
      721 gcgactcgcg ggcctcctcc atccgccgcc gcgcgacgcg gcccgtcccg tgcagtgacg
      781 acgcattgcc gaccgtggcc agcaccgccg tcatcgcctc gatggcagca gggtgcatcg
      841 gggtggtggc ggcgtgatcg aggtagaccg tgtcgtgcga ggtcataacc gttccatgct
      901 agcggccatg gaacggcagg cccgaatccg aggttcaggc caccagagcg tgaccgtgac
      961 cacgcagggt cgcgtcgccc cgcaccgcgg cctcgcaccg ggccgccaga tcccgccgtg
     1021 acgttcctgg cagctgcagc gactcgacct gcacgtgaac caccgtgcgg cgggcggtca
     1081 ccagccgccg gatggatgcc atcaacgtgt cgtcgccgac ataagcggcc accgtcgacg
     1141 gcctgccgtc gcgatggtgg tagctcagcc gcagtggctg cactggtcgt cccgcgtcga
     1201 cggcggcctg gaacatcgcc ggccggaagg ggccgtatgc gaggccgcac cacgtggtgc
     1261 cctcggggaa cgcgacgatt gtctggcccg cgcgcagccg ggcggcgacc gtgcccacca
     1321 cgtcgggcag acgcctcagg ctcgcacggt cgatcggaat caccttcatc aggcgggcca
     1381 ccattcccag gccgatccag tcgacgaggt cggcgcgggc gacgaacctg ccgggcatca
     1441 ccgcgccgat cgcgaagatg tccacccagg acacgtgtcc gctgaccaca agcacaccac
     1501 gcaagttacg aatgggcccg ccggacaccg tgattcgcac gccgaggcag cgcagcatca
     1561 tcctgcagta gccccgttgg atgtgtgagc ggccgggcat cgggacggcc agcaagggcg
     1621 atcccgacaa caggatcagc gcgaaggtgg cccgcatcgc gatgcgccag gcgacgacca
     1681 cgggctggcc gggcgccact gcgtcagcgc tgacacaggt ggcgtcacag gtcgcccgtg
     1741 gcagccaggc atgttcgatc gtcacgacga catcccgtcg gccatcgcgg ccgccgcccc
     1801 taccgagcgc agcctgcgca ggtagcggac gtcggcgtgg cgtttgtcca gcagcgccgg
     1861 gaagtcgccg accccgaagt cggggtcgtg ggccggatct ccgcacacct ttgcgcccag
     1921 tcgcaggtag ccccgcatca gcggcggcac gctcacccgg gccggcgggt cgatgtcgtc
     1981 gaggcccttg ccgccgatcg tcaccggccg gtacgggtgc acggtgtagg gcgcggcgtg
     2041 gcggcggcgc acgaagtcgc gcacaccacg gatctgcctg cccggcgcct ccccgtcggc
     2101 gctgtccacc ggcaccgaca cgcagccggc gacgtagtcg tatccgcacc ggtcgaggta
     2161 ggccaggatg cctgcccaca tcagcagcac cacgccgccg ttgcggtgat cctcgcggac
     2221 cacggcgcga cccatttcga ccagcgaggg ccgcagcgaa tccagctccc gcacgtcgaa
     2281 ttccgttgcg gtgtaaagac ccccggccgc gatggccccg ggcggcggca gcatccggta
     2341 gcagccgacc agttcgccgg agttgtcctc gcggaccagc aggtggtcgc agtgttcgtc
     2401 gaagcggtcg gcgtcacgac catcggtggc gccggccaac gcgaatccgg gttcggaggt
     2461 gaacacgtcg tgacggaggc gctgcgcggc gtcaatgagc gctgggtcgg tggacagcag
     2521 cagggtgtag cggggggtat cggctgatgg ggtcgtcgga tctgcggcga tgagtacaga
     2581 agcagtgctc atagccagca cggtcgccga accgcatctc gggacggcat cgtggcggtg
     2641 acgtgtccgt gagcggtagg tgacgacttg tggcggtcag acgtcagctg tccaggaaca
     2701 ccacgttctt atcgacctga tttcgcggta cagcgaggct gtttcccggg aaggcggggt
     2761 ccggataacc gatggctaac ccgcagagca cggtcaggtc gtccggtatg tccaggtggg
     2821 cccgcaggat ctccgggtag gccgcgatcg acacctgcac gcaggagccg agaccgcgtt
     2881 cggtcaacgc cagcaccagc gtctgcagga acatgccgac gccgagggcg tcgaccagcc
     2941 ccagatcgcg gtgcatgcac accacggcgc cgacgggcgc gcggaagaac tcccagttgc
     3001 gcagctgggc cagcagccgc gcctccgcgt cgtggcgcgc gatgcccatg gcgccgtaga
     3061 ccagtgcgcc gagttcctgc cgcagcggga tgaagtgctc cgggagaccg gtggtgaccg
     3121 gcaactcgac ggatgcctcc tcgaggagcg cttcgaccaa ccggtcgcgg cggggccccg
     3181 aggcgatgaa cactcgccac ggctggacat tggagttcga cggcgcgcgc atcgcaagct
     3241 gcagcgcttc gtcgagcagt tcacggggta ccggcttgtc gcgcaggaac agccgtgtcg
     3301 atcggcggtc gcgcacgacc tcgcccaatt cggtcatcgt cgtgggacgc gccggtcctt
     3361 acgcacctcg agttcgtcct catcgacgaa gaccagcatc ggcgcgccag gcgtgcacac
     3421 catcgtgacc acgtaccgga gcgggatatc ggagcggttg ttggcgtccg aatagtggat
     3481 ggcgtcaccg cccggctccc agaacgcctc accggccttg atcacccgcg gcgcttcgcc
     3541 ttccagctcg aagagcatct cgccttccag tacatagccg aatgacgggc cgccggggtg
     3601 gcggtgcggc ggcgcgcctg cgcttccggc aggccactcg atcacaatag tcatcacctc
     3661 agcatcggaa gggatgaacg gcggtgtgac ggtctggatg gtcgagacgg ctttcgccag
     3721 atcggcggcg tcgaacattg tttctcctca gtcgaattcg gatcggttag gcggtgacca
     3781 ggctgtgctg ctggagccgt gtaccgaagt aggtggcttg cgggtcgacc acgacggtct
     3841 tgtcgacgcc ctgcttggcg agcacgctgc gggcgaggtc ggcgaacgag atcttctccg
     3901 gcccaccgat gttgacgaca ctgtcggccg gtccggcgac cgcggcgcgg gccacctcgg
     3961 cggcgacgtc gtcggcggcg atcggctgga tcagcgcgtc ggggacgcgc acctcatcgc
     4021 cggcgtccag cgatcccacg atcgccgcgg cgaactcctg gaactgggtg gcccgcacga
     4081 tcgtgtacag cagccccgac tcggcgatcg tcttctcctg caccactttt gcgcgcatgt
     4141 agccgctctc gggcaggccg tccactccga cgatcgacaa cgcgacgtag tggccgaccc
     4201 cggactcctt ggcggctgcc accaggttcg ccgatgagcg ggtgaagaag tccatcaccg
     4261 ggtcgtcgtc gaacgacggt gaattggtca ggtcgacgag gacgtcggcg ccggccagcg
     4321 cgtcggcgag tccctcgcct gtgagcacgt cggcgcccga cgcgcgcgac gcggcgacga
     4381 cgtcctggcc gtcacccttg agtaggtcga ccaccttcga gccgatcagg ccggtggctc
     4441 cgataactga gatcttcatg cgtccagcct gacgctcgcg gcccgtggtc gagcccttga
     4501 aagtccgtag tcttcatggc gagaagacgc aaaaacccct tacgttggtg tcgggggctt
     4561 ttccgtctgc tcgcgcagag aagaactaac ccttgcgggc cttgaccgcc tcggtcagct
     4621 gcggggcgac cttgaacagg tcaccgacga taccgaggtc ggcgatctcg aagatcggcg
     4681 cctcttcgtc cttgttcacc gcaatgatcg tcttcgacgt ctgcataccg gcgcggtgct
     4741 ggatcgcccc ggagatgccc agcgcgatgt acagctgcgg cgacacggtc ttaccggtct
     4801 ggcccacctg aaactggccc gggtagtagc cggagtcgac cgcggcacgc gacgcgccga
     4861 cagccgcgcc gagcgagtcg gccaattcct ctaccacgct gaagttttcg gcgctgccga
     4921 cgccgcggcc gccggagacg acgacggtgg cctcggtcag ttccggacgg tcgccggcga
     4981 ctgcgggctc acgcttgatg atcttcgtcg cattctcggc ctgcgcgggc acctcgacgt
     5041 tgacgacctc accggcaccc gccgcgggct cggcctcgat cgcacccggg cgcacggtga
     5101 tcaccggggt gtcgccggtg gcctcggcct ccacggtgaa cgcgccaccg aagatcgagt
     5161 ggacggcctt gccgccgtcc ttgacctcga cgacgtcgac gagcagaccg gcgccggtgc
     5221 gggcggccag ccgacccccg atctccttgc cgtctgcgct ggcggccagc agcacaccag
     5281 ccggagcagc cgactcggtc agcgcggcca gcacatccac gtaaggggtg atcaggtagt
     5341 tctccgcgtc gtcggactcg gcgacgtaga tcttggcggc gcccgcctcc ttcaacgcgt
     5401 cggtcagcgg cgcagcggtg cccggcttgc ccaccacgac ggcggcgggc tcgcccagct
     5461 tgcgggcggc ggtgatcagt tcggcggtga tcttcttcac cgcgccatcg gcgtgctcga
     5521 cgagcacaag tacttcagcc atgtgtcttc tatgtctttc gctgtctgag gaagtggtct
     5581 agatgatctt ttgggcaacc aggtactcgg cgaccttcgt gccgccatcg ccctcgtcgg
     5641 tgatcttctc tccggccgtc ttcggcggct tcggggtcga cgcagtcacc ttggaaccgg
     5701 cgttcgcgac gccgacctcg tcggcctcga caccgatctc cgccagcgtc agcgtcgtca
     5761 cttccttctt cttggcggcc atgatgccct tgaaggacgg gaagcgcggc tcgttgatct
     5821 tctcgttgac cgacaccacc gcgggcagcg gcgcctcgac agtgaacacg ccgtcatcgg
     5881 tctcgcgctc cccggtgacc ttgccgccct cgaccgacac cttccgcagg tgggtcagct
     5941 ggggcaggcc caggtattcg gcgatgaccg cgggcaccgc tccgccgacg ccgtcggtgg
     6001 cctcgttacc ggcgatcacc agctcggtgc cctcgatggt gcccagcgcg cgggccagcg
     6061 cccagccggt ctggatcagg tcggaaccgt gcaggccgtc gtcgagcagg tgcaccgcct
     6121 tgtcggcgcc catcgacagg gccttgcgaa tggcctcggt cgcgcgcgcg gggcccgctg
     6181 tgagcactgt cacagtgctc tcggtgccgt tggcggcttc cttctccttg atcagaagcg
     6241 cctcttcgac ggcgcgctcg ttgatctcgt cgagcacggc gtcggcggcc tcacggtcga
     6301 gggtgaaatc gccgtcggtc agcttgcgct ccgaccacgt gtctgggacc tgtttgatca
     6361 ggaccacgat gttcgtcatg agtctggttc gtcctcctcg gagaggccgg cggccggcct
     6421 gcaataccac gtttcaagca actagcacca tgttactcgc gggtaactta tggtggttcg
     6481 caggatcacc atagctggtc gaagtttgtg ttctagcagc ccgtagcacc tgaaccgttc
     6541 gccatcgggc cgatccgcgt tagcctgcgt tgcaatgagc gcctcattcg ttaccaacgg
     6601 tccgcttggg gacgatgcgt tgcctctcac gggcgaacgc acgattcctg ggctggcgga
     6661 ggaaaactac tggttccgcc gccacgaggt ggtgtacgag cggctggccg gtcgctgcgc
     6721 agatcgcgac gtgctcgagg cgggctgcgg cgagggctac ggcgccgacc tgatcgccga
     6781 cgtggcgcgc cgggtgatcg ggctggatta cgacgagtcg gccgtcgcgc acgtgcgggc
     6841 ccgctatccg cgggtggaga tgctgcacgg caacctggcc gagctcccgt tgcccgatgc
     6901 cgccgtcgat gtggtcgtca acttccaggt gatcgagcac ctgtgggatc aaggccagtt
     6961 cgtggccgaa tgctttcgcg tcttgcgacc gggcggtgcg ctgctgatct cgacgccgaa
     7021 ccggatcacc ttctcccccg gccgcgacac ccccatcaac ccgtttcaca cccgcgaact
     7081 caacgccgcg gagctgacgg agctgttgac gaccgcgggc tttgcggtcg aggccatgct
     7141 gggtgtcttt catggttcgc ggctggccga gctcgacgag cggcacggcg gctcgatcat
     7201 cgacgcgcag atcgcccgcg cgctcgccga tgcgccctgg cctgaggacc tgctcgccga
     7261 tgtcgcttct gtggcgacgg atgacttcga tgtcgttgat gatcgcgata tcgatgacag
     7321 cctcgacttg gtcgcaatag cggtgcggcc gtgacgaatt ccgagcgcgt acccgggctg
     7381 ttcacgctcg tactgcacac gcatctgccg tggctggccc atcacgggcg ctggccggtc
     7441 ggtgaggaat ggctctatca gtcgtggtcg gcggcctacc tgccgctgat gcgggtgctg
     7501 cgcacgctgg ccgacgaagg gcgcacgcgc gttgtgacgc tcggtatgac gccggtggtc
     7561 accgcgcagc tcgatgaccc gtactgcctg tcgggcatgc atcattggct ggccaactgg
     7621 cagctgcggg cgctggaggc aaccacgctg ggtgacccgt tgcgggagtt cgggattcgt
     7681 gaacataccg aggccggtca cgcgctggag gagtacgcga cgctgtggcg acacggcgga
     7741 agtccgctgc tgcgcgtgct gatcgacgca ggcaccatcg aattgctcgg cggcccgctg
     7801 tcgcatccgt tccagccgtt gctcaatccg cggctgcgcg agttcgcgtt gcgtgagggc
     7861 ctggccgacg ccggtcagcg gttcgcgcac accccgcgtg gcatttgggc gccggaatgc
     7921 gcgtacgcgc ccggcatgga aatcgattac gccgcagcgg gtgtcggcca tttcatggtg
     7981 gacggcccgt cgttgcacgg cgacaccgcg ttgggccgcc cggtcgcctc gtcggatgtc
     8041 gtcgcgttcg gccgcgatct gcaggtcagc taccgcgtgt ggtcgccgaa gtccggttac
     8101 cccggccacg ccgcctaccg cgacttccac acctacgatc acgtgaccgg cctcaagccc
     8161 gcgcgggtaa cgggccgcaa cataccgtcg gatgccaagg cgccctacga cccccagcgc
     8221 gcagaccgcg cggtcgacgt gcacgtcgcc gacttcgtcg gagtggtgcg cgaccggctc
     8281 atcagcgaat ccgagcggat cggcaggccg gcccacgtgg tggcggcgtt cgacaccgag
     8341 ttgttcgggc attggtggta cgagggcccg gtgtggcttg agcgggtgct gcgtgcgctg
     8401 ccggaggcag gggtgcgggt gggcacgctg tccgacgcgg tcgccgacgg ctttgtcggc
     8461 gcacccgtcg aattgccgcc cagctcttgg ggatccggca aagactggca ggtctgggcg
     8521 ggcgagaagg tcgccgatct ggtgcagctg aacacggaag ttgtcgacac cgcgttgact
     8581 acggtggaca aggcgctggg cgagaccgac gcgccgccgg cccgcaactt cgtcgccgac
     8641 cagatcctgc gcgaaacgct gctcacggtg tccagcgact ggccgttcat ggtgagcaag
     8701 gattccgccg ccgactacgc gcgctaccgc gcacacctgc acgcgcacgc cacccgcgag
     8761 atcgcgggcg cgctggcctc cggccgcaat gagcacgcac agcgcctcgc cgacaattgg
     8821 aaccgcgcgg acggtttgtt cggcgcgctc gatgcaaggc gactcccccg atgaaagttc
     8881 ttatcgtgtc gtgggagtac ccgccggtgg tgatcggtgg gctcgggcgg cacgtataca
     8941 acttggcgac ggcgctggcg gcggccggac acgaggtggt ggtgctcaac cgtcgacccg
     9001 gcggcaccga cccgagcacg catccgtcga ccgacgaaat cgccgagggc gtacgggttg
     9061 tcgccgccgc gcaggaccca cacgagttcg acttcggtgc cgacatgatg gcgtggacgc
     9121 tggccatggg gcattcgatg gtccgcgccg gcctgggcat ccgggaccga cgcggccggc
     9181 cgtggcgtcc cgacgtcgtg cacgcccacg actggctggt cgctcatcct gcgatcgcgc
     9241 tcgccgaata cctcgacgtg ccactg
//
LOCUS       DASRJA010000092         7014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1051285,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000092 DASRJA010000000
VERSION     DASRJA010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051285"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFC01_04105"
     CDS             complement(<1..197)
                     /locus_tag="VFC01_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78863.1"
                     /translation="MNALRRAAQVFVVATALTALAVIGVSGVAGADPGIGWGAPGPGI
                     LPGPGLGGWGGPASGLGVLPGL"
     gene            complement(279..593)
                     /locus_tag="VFC01_04110"
     CDS             complement(279..593)
                     /locus_tag="VFC01_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HZN78864.1"
                     /translation="MIFKGVREGKPYPEHGLSQRQWAQIPPRQIRLDELVTTTTVLAL
                     DRLLSEDSTFYGDLFPHAVRWKGIVYLEDGLHRAVRAALRNRTVLHARVFDMDMPVAQ
                     HA"
     gene            739..1770
                     /gene="hrcA"
                     /locus_tag="VFC01_04115"
     CDS             739..1770
                     /gene="hrcA"
                     /locus_tag="VFC01_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZN78865.1"
                     /translation="MGSADDRRFEVLRAIVADFVATKEPIGSKSLVERHNLGVSSATV
                     RNDMAVLEAEGYIAQPHTSSGRVPTEKGYREFVDRLDDVKPLSSSERRAILTFLESGV
                     DLDDVLRRAVRLLAQLTRQVAIVQYPTLSTSSVRRLEVVALTAARLLLVVITDSGRID
                     QRIVELGDAIDDHELSQLRDRLGQAMEGKPLAAASIAVSDLASHLNGTGGLADAVGRA
                     ATVLVETLVEHSEERLMLGGTANLAQNTADFGGSLRSVMEALEEQVVVLRLLAAQQEA
                     GKVTVRIGHETEAEQMAGTSVVSTAYGSSGKVYGGMGVLGPTRMDYPGTIANVAAVAL
                     YIGEVLGSR"
     gene            1793..2935
                     /gene="dnaJ"
                     /locus_tag="VFC01_04120"
     CDS             1793..2935
                     /gene="dnaJ"
                     /locus_tag="VFC01_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZN78866.1"
                     /translation="MARDYYGMLGVSRGASDSEIKRAYRKLARELHPDVNPDEQAQAR
                     FKEISVAYEVLSDPEKRRIVDLGGDPLESAAAAGNGFGGFGGLGDVFEAFFGGGTTSR
                     GPIGRVRPGSDSLLRMRLDLEECATGVTKQVTVDTAVLCDLCQGKGTHGNSTPVACDT
                     CGGRGEIQTVQRSLLGQVMTSRPCPVCGGVGEVIPDPCHRCGGDGRIRARRDISVKIP
                     AGVGDGMRVRLAAQGEVGPGGGPAGDLYVEVHEKQHELFIRDGDDLHCTISVPMVDAA
                     LGTTVTVDAIIDGPTELTIPAGTQPGAVTTLRGHGMLHLRSGVRGDLHAHIEVMVPQR
                     LDHEDIELLHKLKEKRIRDVAEVRSTQSTATGSGFFSRLRETFTGR"
     gene            2942..3682
                     /locus_tag="VFC01_04125"
     CDS             2942..3682
                     /locus_tag="VFC01_04125"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HZN78867.1"
                     /translation="MTRALFYVDALPEVGELAIVDGDEGFHAANVRRIRLGEEIDLGD
                     RDGTVAHCVVEDVARGRLTARVLDRGTVPVPSPTVTVVQALPKSDRSELAVELATEAG
                     ADAFIAWQASRCVARWDGSAKVDKGLRRWAAVARSAARQSRRPYVPSISGVVSTAELV
                     QRVRDDTAMGLVLHESATVTLTEVPVAQVDSILLVVGPEGGIADDEIAALTDAGATPV
                     RLGPTVLRTSSAAAVALGALGALTSRWS"
     gene            3788..4819
                     /locus_tag="VFC01_04130"
     CDS             3788..4819
                     /locus_tag="VFC01_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZN78868.1"
                     /translation="MTLRENADSDSPVRSSINVPPDLVVGLLGSADENLRVLERILAA
                     DIHVRGNALTLSGEPADVALAERVISELMAIVASGQRLTPDAVQHSVAMLTGTGDESP
                     AEVLTLDILSRRGKTIRPKTLNQKRYVDAIDAHTIVFGIGPAGTGKTYLAMAKAVNAL
                     QSKQVNRIILTRPAVEAGERLGFLPGTLSEKIDPYLRPLYDALYDMMDPESIPKLMNA
                     GIIEVAPLGYMRGRTLSDAFIVLDEAQNTTGEQMKMFLTRLGFNSKIVVTGDITQVDL
                     PGGAASGLRAAMRILDGIDDIHFSELTSADVVRHRLVSDIVDAYAKSEEPAQMNRAQR
                     RASGTRSRR"
     gene            4824..5360
                     /gene="ybeY"
                     /locus_tag="VFC01_04135"
     CDS             4824..5360
                     /gene="ybeY"
                     /locus_tag="VFC01_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZN78869.1"
                     /translation="MSIEVSNESGIDVSEQELISVARFVIRKMKINPAAELSMVLLDT
                     AAMADLHMRWMDLPGPTDVMSFPMDELEPGGRPDAPEPGPSMLGDIVLCPEFASQQAT
                     AAGHTLGQELALLTVHGVLHLLGYDHAEPDEEKEMFALQRQLLEEWVADQVESYHQDR
                     QSEKDRRLLDKSRYFDEP"
     gene            5357..6643
                     /locus_tag="VFC01_04140"
     CDS             5357..6643
                     /locus_tag="VFC01_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZN78870.1"
                     /translation="MTGIAPLIGAIALIGFGGLFAAIDAAISTVSVARVEELVRDERP
                     GAVRLARVMVERPRYINLVVLLRITCEVTATVLLVAYLDGLLGVAWGLFAAAAIMVVV
                     SFVAIGVGPRTVGRQNAYSIALVAALPLQAISVLLTPISRLLVLIGNALTPGRGFRNG
                     PFASEIELREVVDLAQQRGVVADEERRMIQSVFELGDTPAREVMVPRTEMVWIESDKT
                     AGQATSLAVRSGHSRIPVIGENVDDVIGVVYLKDLVQRTYYSRNGGRDTKVSEVMRPA
                     VFVPDSKPLDALLREMQRDRNHMALLVDEYGAIAGLATIEDVLEEIVGEIADEYDTDE
                     VAPVEDLGDKHFRVSARLPIEDLGELYDIEFDEDLDVDTVGGLLALELGRVPLPGAEV
                     MWDGLRLRAEGGPDPRGRVRIGTVLVSPTEPEEPHE"
     gene            6636..6953
                     /locus_tag="VFC01_04145"
     CDS             6636..6953
                     /locus_tag="VFC01_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HZN78871.1"
                     /translation="MSELDAEDAKLVTLARGAMGRAEATTGAAVRDVDGRTYAGAPIR
                     LDALTLTALQAAVAAAVSSGAAGLEAAVIVGGSSDDAGVAAVRELSSDAKVIVTDRSG
                     AVR"
BASE COUNT         1172 a         2238 c         2443 g         1161 t
ORIGIN      
        1 agaccgggca gcacgcccag cccggacgcg gggccgcccc aaccgccgag gcccgggccg
       61 ggaaggatcc ccggtcccgg tgcgccccat ccgatcccag gatccgcacc ggccacgccg
      121 gacaccccaa tcacagccaa tgccgtcaat gcggttgcga ctacgaaaac ctgtgctgct
      181 cttcgtaatg cgttcatgtg ttctcctatc gagtcgggac gtgcacgtcg tcgagcacac
      241 gacgatcgtg acgccaaccc tgtttggaga gcgtgaggct aggcgtgctg cgcgaccggc
      301 atgtccatat cgaagacgcg tgcgtgcagc acggtgcgat tccgcaacgc cgcccgcacc
      361 gcgcggtgca aaccgtcctc taggtagacg atgcccttcc accggaccgc gtgtggaaac
      421 aggtcgccat agaacgtgga gtcctccgac agcaaccggt ccagcgccag cacggtcgtg
      481 gtggtgacca attcgtcgag gcggatctgc cggggcggaa tctgtgccca ctgccgttgg
      541 gacaggccgt gctctgggta tggcttgccc tcgcggactc ccttgaagat catcggctag
      601 caatccctcg cgtctttccc cggcgttaaa tgctagtcgc aaccggccta ttcgccacgc
      661 gaagaaccgc tcgctcggta tccgccctga tgatcaccgt aaaataggga ccgactgcgt
      721 gagaagggca ggtgaacaat gggcagtgcc gacgatcgtc gcttcgaggt gctgcgcgcc
      781 atcgtcgccg acttcgtggc caccaaggag cccatcggct cgaagtcgct cgtcgagcgg
      841 cacaatctcg gcgtctccag cgcaaccgtg cgcaacgaca tggcggtcct ggaggccgag
      901 ggctacatcg cccagccgca caccagctcg ggccgggtgc ccacggagaa gggctatcgc
      961 gaattcgtcg accggctgga cgacgtcaaa ccgctgtcgt cctcggaacg gcgcgcgatc
     1021 ctgaccttcc tcgagtccgg cgtcgacctc gacgacgtgc tgcgccgcgc cgtccggctg
     1081 ttggcccagc tcacgcgcca ggttgcgatc gtgcagtacc caacgctgtc cacgtcgagc
     1141 gtgcggcgcc tcgaggtggt cgcgttgacc gcggcccgcc tgctgctggt ggtgatcacc
     1201 gactcgggcc gcatcgacca gcgcatcgtg gagctcggcg acgccatcga tgaccacgag
     1261 ctgtcccagc tgcgggaccg gctcggtcag gcgatggagg gaaaaccgtt ggccgccgcc
     1321 tcgatcgcgg tgtccgacct cgcctcgcac ctcaacggca ccggcgggct cgccgacgcg
     1381 gtcggccggg cggctaccgt gctggtggag acgctggtcg aacacagcga ggagcggcta
     1441 atgctcggcg gcaccgccaa cctggcccag aacaccgcgg acttcggcgg ctcgctgcgg
     1501 tcggtgatgg aggcgctcga agagcaggtg gtcgtgctgc gcctgctcgc ggcccagcag
     1561 gaggccggca aggtcaccgt ccgcatcggg cacgagaccg aggctgagca aatggcgggc
     1621 acgtcggtgg tcagtaccgc ctacggcagc tcgggcaagg tgtacggcgg catgggtgtg
     1681 ctgggaccca ctcggatgga ctatccggga actatcgcta atgtcgctgc ggttgctctc
     1741 tatatcggcg aagttctagg tagccggtaa ttcggttttg ggaaggtaag gcgtggcacg
     1801 cgactactac gggatgctcg gcgtgagccg gggggcgagc gattcggaga tcaagcgcgc
     1861 ctatcgcaag ctggcccgcg aattgcatcc cgacgtcaac cccgacgaac aggcccaggc
     1921 gcggttcaag gagatcagcg tcgcctacga ggtgctcagc gacccggaga agcgccgcat
     1981 cgtcgacctc ggtggtgacc cgcttgagtc cgcggccgcc gcgggcaatg gtttcggcgg
     2041 gttcggtggc ctcggcgacg tgttcgaagc cttcttcggt ggcggaacca cctcccgcgg
     2101 cccgatcggc agggtccggc caggctcgga ctcgctcctg cggatgcggc ttgacctgga
     2161 ggaatgcgcc accggcgtga ccaagcaggt caccgtcgac accgcggtgc tgtgcgacct
     2221 ctgtcagggc aagggcaccc acggcaactc gacaccggtc gcctgcgaca cctgcggtgg
     2281 gcgcggcgag atccagaccg tccagcgctc gctgctcggt caggtgatga cgtcgcggcc
     2341 gtgccccgtg tgcggtgggg tcggcgaggt catccccgat ccgtgccacc gctgcggcgg
     2401 cgacggccgg atccgggcgc gacgggatat cagcgtgaag atccccgcgg gtgtcggcga
     2461 cggaatgcgg gtccggctgg ccgctcaagg cgaggtcggt ccgggtggcg ggcctgccgg
     2521 cgacctgtac gtcgaggtgc acgaaaagca gcacgagctg ttcatccgcg acggtgacga
     2581 cctgcactgc acgatctcgg tgccgatggt cgacgccgcg ctgggcacca cggtcaccgt
     2641 cgacgcgatc atcgatggcc cgaccgagct cacgattcct gcgggcacgc agcccggtgc
     2701 ggtgacgacg ctgcgcgggc atgggatgct gcatctgcgc tccggggtgc gcggcgacct
     2761 gcacgcccac atcgaggtca tggtgccgca acggctcgac cacgaagaca tcgagctgct
     2821 gcacaagctg aaggaaaagc gcatccgcga cgtggccgag gttcgctcga cccagagcac
     2881 ggcgacgggt agcggattct tcagccgctt gcgcgaaacg ttcactggcc gctgattttc
     2941 tgtgactcgc gcgctcttct acgtcgacgc gctgcccgaa gtcggtgagc ttgcgatcgt
     3001 cgacggcgac gagggatttc atgcggctaa cgtccggcgc attcgtctcg gtgaggagat
     3061 cgaccttggc gatcgcgacg ggacggtggc ccactgcgtt gtcgaggacg ttgccagggg
     3121 ccggttgacg gcgcgggtgc tcgaccgggg aacggtgcca gtgccgtcgc cgactgtcac
     3181 cgtcgttcag gcactgccca agtccgaccg ctccgagctg gccgtcgaat tggccaccga
     3241 agccggggcc gacgcgttca tcgcatggca ggcgtcgcgc tgtgtggccc ggtgggatgg
     3301 gtctgcgaag gtcgacaagg gtctgcgccg gtgggccgca gtcgcgcggt cggcggctcg
     3361 ccagtcgcgg cgtccgtacg ttccgtcgat cagcggtgtg gtgtcgacgg ccgagttggt
     3421 gcagcgggtg cgtgacgaca ccgcaatggg gctggtgctg catgagtcgg ctaccgtcac
     3481 gctgacggaa gtgcctgtgg cacaagtcga ttcgatctta cttgtcgtcg gccccgaggg
     3541 gggcatcgcc gacgacgaga tcgctgcgct gaccgacgcg ggtgcgacac cggtgcggct
     3601 ggggccgaca gtcttgcgga cctcttctgc tgcggccgtc gcgctgggcg cgttgggcgc
     3661 gctgacctcg cggtggtcgt gagctggtgc tggttaagca ttgggatgca tcgctacccg
     3721 gcggtaaaat gggtattcaa gagcaaacga ccaggccaga acccagaaag caggcagaaa
     3781 accccacgtg acgctccgcg agaacgctga ctcggactcg cccgttcgta gcagcatcaa
     3841 tgttccgccc gacctcgtcg tgggcctgct tggttccgcc gacgagaatc tgcgcgtact
     3901 cgagcgcatc ctcgcagccg atatccacgt ccgcggcaac gcgctgacct tgtccggcga
     3961 gccggccgac gtggcgctgg ccgagcgggt gatctccgag ctcatggcga tcgtggccag
     4021 cggtcaacgg ctgacaccag atgcggtgca gcacagcgtc gcgatgctga ccggcaccgg
     4081 cgacgaatcg cctgcagagg tgttgacgct cgacatcctg tcgcggcgcg gcaagaccat
     4141 tcggcccaag acgctgaacc agaagcggta cgtcgacgcg atcgacgcgc acaccatcgt
     4201 gttcggtatc gggccggccg gcaccggcaa gacttatctc gcaatggcca aggcggtcaa
     4261 cgcattacag agcaagcagg tcaataggat catcctcacc cggccggccg tcgaggcagg
     4321 cgagcggctt ggcttcctgc ccggcacgct tagtgagaag atcgacccgt atttgcggcc
     4381 gctgtatgac gcgctgtacg acatgatgga tcccgagtcg atcccaaagc tgatgaacgc
     4441 cgggatcatt gaggtggcac cgctagggta catgcgtggt cgcacgcttt cggacgcgtt
     4501 cattgtcctc gacgaggcgc agaacaccac cggcgagcag atgaagatgt tcctgacccg
     4561 gctcgggttc aactccaaaa ttgttgtgac aggcgatatt acgcaggtcg atctgccggg
     4621 cggtgccgct tcgggtctgc gcgcggcaat gcgaatactc gacggcatcg acgacattca
     4681 cttctccgaa ctcaccagcg ccgacgtcgt gcggcaccgg ctggtctccg acatcgtcga
     4741 cgcctacgcc aaatccgagg agcccgcgca gatgaaccgg gcccagcggc gcgcgtcggg
     4801 cacccgatcg cgaaggtgag cgcgtgagca tcgaggtgtc caacgagtcg ggcatcgacg
     4861 tttccgaaca ggaattgatc agcgtcgcgc ggttcgtcat ccgcaagatg aagatcaacc
     4921 ccgcggccga actgtcgatg gtgctgctcg acaccgcggc gatggccgac ctgcacatgc
     4981 gctggatgga cctgccggga ccaaccgacg tgatgagctt cccgatggac gagctcgaac
     5041 cgggcgggcg gcccgacgcg cccgaaccgg ggccgtcgat gctcggcgac atcgtgctgt
     5101 gcccggagtt cgcgtcccaa caggccaccg cggccgggca cacgctgggc caggaactgg
     5161 cgctgttgac cgtccacggc gtgctgcatc tgctcggcta tgaccacgcc gagccggacg
     5221 aggaaaagga aatgttcgcg ctgcagcgcc aactgctcga ggagtgggtg gctgaccagg
     5281 tcgagtcata ccaccaggac cgtcagtccg agaaggaccg gcggctgctg gacaagtccc
     5341 gatacttcga cgaaccgtga ccggcatagc cccgctcatc ggagcgatcg cactcatcgg
     5401 tttcggcggg ctgttcgcgg ctatcgacgc cgctatcagc acggtgtcgg tggctcgtgt
     5461 cgaggaactg gtgcgcgacg aacgcccggg cgcggtgcgg ctggctcgtg taatggtgga
     5521 gcggccgcgg tacatcaatc tcgttgtgct gctgcgcatc acatgcgaag tgacggcgac
     5581 ggtgttgctc gtcgcctacc tggacgggct gctcggcgtg gcctgggggc ttttcgcggc
     5641 cgctgcgatc atggtggtgg tcagcttcgt cgcgatcggt gtcggtccgc gcacggttgg
     5701 gcggcagaac gcctacagca tcgcgttggt ggccgccctt ccgctgcagg cgatctcggt
     5761 gttgctcacc ccgatcagcc ggcttctggt gttgatcggt aatgcgctga cgcccggtcg
     5821 agggttccgc aacggaccgt tcgcctccga gatcgagcta cgtgaggtcg tcgacctggc
     5881 ccagcagcgc ggtgtggtgg ccgacgaaga gcgccggatg atccagtcgg tcttcgaact
     5941 cggcgacact ccggcccgcg aggtgatggt gccgcgcacc gagatggtgt ggatcgaaag
     6001 cgacaagacg gcgggccagg cgacgtcatt agcggtgcgc agtgggcatt cgcggatccc
     6061 ggtgatcggc gagaacgtcg acgacgtcat cggtgtcgtc tacctcaaag accttgtgca
     6121 gaggacgtat tactcgagaa acggcggtcg cgacaccaag gtgtcagaag tgatgcgccc
     6181 cgcggtgttc gtcccggact ccaaaccgct cgacgcactg ctgcgcgaaa tgcagcgcga
     6241 tcgcaaccac atggcgctgc tggtcgacga atacggcgcc atcgcgggcc tggcgacgat
     6301 cgaggacgtg ttggaggaga tcgtcggcga gatcgccgac gagtacgaca ccgacgaggt
     6361 tgcgccggtg gaagacttgg gcgacaagca ttttcgagtg tctgctcggc tgcccatcga
     6421 ggatcttggc gagctctacg acatcgaatt cgacgaggat ctcgacgtcg acacggtcgg
     6481 cggcctgctg gccctcgagc tgggccgcgt tccgctgccg ggggcggaag tgatgtggga
     6541 cgggctgcgg ctgcgcgccg aaggcggccc ggaccctcgc ggtcgggtgc gcatcggtac
     6601 ggtcctggtc agtccgacgg agccggagga gccccatgag tgaactcgac gccgaggacg
     6661 ccaagctcgt gacgttggcc cggggtgcaa tgggccgggc ggaggcgacc accggcgcgg
     6721 cggtgcgtga cgtcgacggc cgcacgtacg ccggcgcacc catcaggctc gatgccctga
     6781 cgttgaccgc gctgcaggcg gctgtcgcgg ccgcggtgtc cagcggcgcg gcgggtctgg
     6841 aggccgcggt aatcgtgggt ggctcgtcgg acgacgccgg ggtggccgcg gtccgcgagc
     6901 tgtccagcga cgcgaaggtg atcgtcaccg accgcagcgg cgccgtgcgg tgagcgaatt
     6961 ccgttcgggc tttgtctgct tcgtcggccg gccgaacacc ggaaagtcga cgct
//
LOCUS       DASRJA010000093        30475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4149927,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000093 DASRJA010000000
VERSION     DASRJA010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 30475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30475
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4149927"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VFC01_04150"
     CDS             complement(<1..820)
                     /locus_tag="VFC01_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561927.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HZN78872.1"
                     /translation="MAASAQGLCEFIDASPSPFHVCVTVAQRLSAAGFTELSERDEWP
                     AQGRFFTVRAGSLVAWQANEVDRSGPFRIVGAHTDSPNLRVKQHPDRFVSGWQVVALQ
                     PYGGAWLNSWLDRDLGISGRLSIRVGNTLDHWLVRIDEPILRVPQLAIHLAEDRKSVS
                     LDPQRHVNAVWGVGSGSRSFLRYVAEHAGVAEADVLGADLMTHDLTPSTLAGANEDLV
                     SAPRLDNQATCYAGLEAFLAAAPGEHMPVLVLFDHEEVGSQSDHGAQSELLHTVL"
     gene            864..1892
                     /locus_tag="VFC01_04155"
     CDS             864..1892
                     /locus_tag="VFC01_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HZN78873.1"
                     /translation="MPAPLPQPVTAPLTPAAIFLVVTIDEGGEAAVHDSLPDISGLVR
                     AVGFRDSDKRLSVITSIGSDAWDRLFSGPRPAELTRFVELDGGRHKAPATPGDLLFHI
                     RAMSMDMCFELAGRLVKAMGAVTVVDETHGFRFFENRDLLGFVDGTENPAGPLAVSAT
                     EIGDEDPDFAGGCYVHVQKYLHNMAAWDSLSVTEQELVIGRTKLEDIEMDDDVKPANS
                     HIALNVIEDEDGNELKIVRHNMPFGEIGKDEFGTYFIGYSRTAAVTERMLRNMFIGDP
                     PGNTDHILEFSTAITGGKFFTPVVDFLNDPPPLPDSAPEEEQPVVTVARVATNGSLGI
                     GSLKGTPS"
     gene            1889..2686
                     /locus_tag="VFC01_04160"
     CDS             1889..2686
                     /locus_tag="VFC01_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HZN78874.1"
                     /translation="MNNLYRELAPITEAAWDQIEEEATRTFKRHIAGRRVVDVSEPGG
                     PVTAAISTGHLRDVGAPGDGVVAHLRESKPLVRLRVPFTVSRTAIDDVERGSQDSDWD
                     PVKDAAKKLAFVEDRAIFEGYDAASIEGIRACSSNPALALPEDARSYPDVIAQALSEL
                     RLAGVDGPYSVLLSADDYTKVSETTEHGYPIREHLNRLVDGDIIWAPAIDGAFVLSTR
                     GGDFDLQLGTDVSIGYLSHDADNVELYLEETLTFLCYTAEASVALTP"
     gene            complement(2711..3385)
                     /gene="purQ"
                     /locus_tag="VFC01_04165"
     CDS             complement(2711..3385)
                     /gene="purQ"
                     /locus_tag="VFC01_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZN78875.1"
                     /translation="MTARVGVITFPGTLDDVDAARAVRLAGAEAVSLWHADADLKGVD
                     AVVVPGGFSYGDYLRCGAIAKFAPVMGEVVRGAEQGLPVLGICNGFQVLCEAGLLPGA
                     LTRNAGLHFVCRDLWLEVVSNSTAWTSRYELGADLLVPLKSGEGRYVAGEKVLDQLEG
                     DGRVVFRYRDNPNGSMRDIAGISSANGRVVGLMPHPEHATEALTGPSDDGLGLFYSAL
                     DLVLAA"
     gene            complement(3382..3621)
                     /gene="purS"
                     /locus_tag="VFC01_04170"
     CDS             complement(3382..3621)
                     /gene="purS"
                     /locus_tag="VFC01_04170"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HZN78876.1"
                     /translation="MARVVVHVMPKAEILDPQGQAIVGALGRLGFGGVSDVRQGKRFE
                     LEVDDSIDDDTLAEIAESLLANTVIEDWSVNREQS"
     gene            complement(3658..4329)
                     /locus_tag="VFC01_04175"
     CDS             complement(3658..4329)
                     /locus_tag="VFC01_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZN78877.1"
                     /translation="MRLLLAVAMATCGLAVAPTGGWPARAVPGVCPPICDAIPDSAWI
                     APTSVPLYPVYRWPGLAGLAVTAPSPRFGFEALCGSPPVASDPRAYAVGARSVVLHED
                     GQWNLLVQVIHWRGDTATGGPTALTTLETARSRLGDCQSTAAVASPSITTDDPQRIGA
                     VISAAGEQVMHEYLLAHPASSTVVELALWSSLPPQVDWPAVADTQVFDALAAPLCDAY
                     LGSCR"
     gene            4376..5029
                     /locus_tag="VFC01_04180"
     CDS             4376..5029
                     /locus_tag="VFC01_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN78878.1"
                     /translation="MELTHFGHSCLLANFKGDSGQETTVLFDPGNFSHGFEGITGLSA
                     ILITHQHPDHADKERLPALVDANPQAALYADPQTAEQLGGPWQAVHVGDEFSVGHLTV
                     RGVGGRHAVIHPEIPVIDNISYLIGDGSHAARLMHPGDALFTPGEPVDVLATPAAAPW
                     MKISEAVEYLRAVAPARAVPIHQAIIEPNARGIYYGRLKEMTDTDFQVLSEETRTSF"
     gene            complement(5026..6669)
                     /locus_tag="VFC01_04185"
     CDS             complement(5026..6669)
                     /locus_tag="VFC01_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HZN78879.1"
                     /translation="MADADVIVVGAGLAGLVAACELVERGRRVLIVDQENANNVGGQA
                     YWSFGGLFFVDSPEQRRLGIRDSHELALQDWLGAAGFDRPEDHWPRQWAHAYVDFAAG
                     EKRSWLRERGLQTFPLVGWAERGGYDARGHGNSVPRFHITWGTGPAIVDVFARKLLGN
                     AKVRFAHRHRVDELIVENGAVTGVRGAVLEPTSAARGVASSRNTVGDFEFHAAAVIVA
                     SGGIGGNHDLVRQNWPTRMGRVPEQLLSGVPAHVDGRMIGITESAGGHVINSDRMWHY
                     TEGITNYDPIWPLHGIRIIPGPSSLWLDAHGKRLPPPLYPGFDTLGTLEYISQTGQDY
                     TWYVLNARIIAKEFALSGQEQNPDLTSKSVRDVLARVRAGAPKPVQAFVDKGVDFVSA
                     NSLRDLVSAMNAVPDVLPLDYATVEAEVTARDREVANRFTKDTQIASIRVARNYLGDR
                     LGRVVAPHRLTDPKAGPLIAVKLHILTRKSLGGLETDLDSRVLRADGSVFGGLYAAGE
                     AAGFGGGGVHGYRSLEGTFLGGCVFSGRAAGRAAAGETA"
     gene            complement(6694..7398)
                     /locus_tag="VFC01_04190"
     CDS             complement(6694..7398)
                     /locus_tag="VFC01_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HZN78880.1"
                     /translation="MAGQLIVSISGISDRTLADVEAFCRQLDERDVPASFLVAPRIKG
                     GYRLDSDAPTVDWLADRRGRGDAIVLHGFDEAATKKRRGEFATLPAHEANLRLMAADR
                     VLEHLGLRTRLFAAPGWTVSQGTVTALPRNGFRLLAGLGGITDLVRQTTVRGRVLGIG
                     EGFLSEPWWCRTLVLSAERTARREGIVRVAVAARHLRRSGPRQAMLDAVDLALMHRCM
                     PSVYHWEPYRALTDAA"
     gene            complement(7462..7953)
                     /locus_tag="VFC01_04195"
     CDS             complement(7462..7953)
                     /locus_tag="VFC01_04195"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZN78881.1"
                     /translation="MTSLTDIPLTTLDGRPTTLAELADGAALVVNVASKCGLTPQYTA
                     LEQLATEYGDRGLTVIGVPCNQFMGQEPGTAEEIQTFCSTNYGVTFPLLAKTDVNGAD
                     RHPLYAKLTQTPDVSGEAGDVQWNFEKFLLAPGGEVVNRFRPRTVPDAPEVIAAIEAV
                     LPR"
     gene            complement(7963..8520)
                     /locus_tag="VFC01_04200"
     CDS             complement(7963..8520)
                     /locus_tag="VFC01_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78882.1"
                     /translation="MSRGTYHHGDLKAVILAKAATLVAERGADGISLRELAREAGVSH
                     AAPAHHFTDRRGLFTALAAEGWRLLADALSGARPDFLDAALAYVRFALAHPGHYAVMF
                     DRSLVNPDDPELVAAVGAAGTELAQGVGTLDDVRAKGDPEAAALAAWSLVHGFSLLWL
                     NQAIDNDPDPMAMVHRVAGMLFKSG"
     gene            8608..9057
                     /locus_tag="VFC01_04205"
     CDS             8608..9057
                     /locus_tag="VFC01_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZN78883.1"
                     /translation="MAVIVTLVLGTVGARLVGWLGVDYVNSWPQAAAVGLAAMFVMTG
                     VAHFVNPLRRDMIAIVPPRLPAPGLLVTTTGVLELVGAAGLLYPPTRVAAAVCLFVLM
                     LVMFPANIYASRMPNPPKSMTSRLDVRTVEEVVYLAAAVVVGLGSIQ"
     gene            complement(9016..11121)
                     /locus_tag="VFC01_04210"
     CDS             complement(9016..11121)
                     /locus_tag="VFC01_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZN78884.1"
                     /translation="MKPPEAKRIDTRREYHGDVFIDPYEWLRDKADPEVLGYLNAEND
                     YTEHATADLAPLRQKIFDEIKARTKETDLSVPIRRGDWWYYGRSFEGKQYSVQCRCPV
                     ADPDDWIPPQFDEDTEVPGEQILLDENVEAEGHDFFALGAATVSLDGNILAYSVDVKG
                     DERYTLRFRDLRTGSLYADEIVGIGSGATWAADNHTIYYVTVDDAWRPDTVWRHRLGA
                     GLPAERVYHEPDEKFWLAVGRTRSDKYIVIAAGSAVTTELRYGDAADGHTEFTAIWPR
                     RELVEYSIEHAVVGGEDRFLIMHNDGAENFTLVEAPVSDPTAFRTLIEHRDDVRLDSV
                     DAFEGHLVVSYRSEALPRIQLWPIYATGEYGRPEDITFDSELMSAGLSANPNWSSPKL
                     RIGATSFVIPVRIYDIDLATGERTLLREQPVLGDYHPDEYVERRDWAVAADGARVPIS
                     IVHRTGVPYPAPTLLYGYGAYESCEDPRFSIARLSLLDRGMVFAVAHVRGGGELGRPW
                     YEHGKLLEKRNTFTDFVAVASHLIATDVTRPDNLVALGGSAGGLLVGAVANMAPQLFA
                     GILAAVPFVDALTTILDPSLPLTVTEWDEWGNPLEDKDVYHYMKSYSPYENIEAKDYP
                     AILAMTSLNDTRVLYVEPAKWVAALRHTKTDDHPVLLKTEMNAGHGGISGRYERWKEA
                     AFQYAWLLDAAKANHDGGG"
     gene            complement(11118..12005)
                     /locus_tag="VFC01_04215"
     CDS             complement(11118..12005)
                     /locus_tag="VFC01_04215"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZN78885.1"
                     /translation="MRPSLSDYEHLASGKVRELYRIDDETLLFVATDRISAYDYILDS
                     EIPDKGRILTAMSVFFFDYVEAPNHLAGPPEDERIPQEVLGRALVVQELEMMPVECVA
                     RGYLTGSGLIDYQKTGQVSGNALPPGLEEASKFAQPLFTPATKAELGQHDENITFNDV
                     IEMVGAVRANQLREKTLQTYIQGADHALTKGVIVADTKFEFGVDKHGNLRLADEVFTP
                     DSSRYWRADDYREGVVQQSFDKQFVRNWLTGPESGWDRNGDKPPPPLPPDIVDATRAR
                     YIEAYERISGLKFSDWIKP"
     gene            12036..12653
                     /locus_tag="VFC01_04220"
     CDS             12036..12653
                     /locus_tag="VFC01_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78886.1"
                     /translation="MRSRYLPYATTPTRLLAQLISDMVVIAWTTVWVLVGMAVHAAVS
                     TIAEVGRQVETGANGVADNLNSAGDRTDDFPLVGDALAKPLKAASEAALDIAGAGHSL
                     DTTASWLAWVLALAVAAPPILFVALPWLFLRIRFFRRKWTALTLAATPAGEQLLALRA
                     LANRPLAKLAAIHADPVGAWRSHDAAAIHGLAALELRAAGVRRRH"
     gene            12755..13621
                     /locus_tag="VFC01_04225"
     CDS             12755..13621
                     /locus_tag="VFC01_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HZN78887.1"
                     /translation="MVDEYLRQHDGVITLAQAMRAGLSHDAVNRRVHSGRWLRCSPGV
                     FFADDRPFTDASRVRTAVWSCGPKATASGLAAAWWHGVTRFAPETVEVTVAKVSNHRR
                     RPGVRTRRRDLAPADIVERKGLRVTALPLTVIEAAVRRGGGPKLMDSALQRHVELPQL
                     WKAHLRNRGRHGSPAARRLLQAASDGARSEAERLLVKLLRDNNITGWKTNYPVGGYKV
                     DVAFPEQKVAIETDGWAYHSSQEDFQNDRVRQNKITLLGWQVLRFTWLDLVEYPQRVL
                     AEIRFARAHPAR"
     gene            complement(13542..14462)
                     /locus_tag="VFC01_04230"
     CDS             complement(13542..14462)
                     /locus_tag="VFC01_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HZN78888.1"
                     /translation="MKPDTFAAVMATGIVSIAAMDHGYRVISDVSIVLAAVALPVLIV
                     AAAIAWRRESWKLTDLDVSLRLCSYVAACAVVAARLAEHRIVLWVLAGMALQGWLSLA
                     PVVARRMWRDRAGLRDRARGGWELASVATSGLAIVMADLKIVFWAVILWALAICIYLV
                     MTGLVVWRAVGDSSAPELAQPDIWILMGGAAVATLAGDHIHKAGLTSVWPVTVVTWIV
                     ATALILPLVYVTLRRRVGIAWPTVFPLGMYSSATYATAVETGWHWLTTVSLAFFWIAF
                     VAWMLTALDALSRIGSPPAPAGDTRLDLAT"
     gene            complement(14459..14938)
                     /locus_tag="VFC01_04235"
     CDS             complement(14459..14938)
                     /locus_tag="VFC01_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN78889.1"
                     /translation="MTFEQRVSELGLQLPPEPLLPPGVRIPFEWARVVGERCVLSGHG
                     ALGSDGAPCGPFGRVPGEVPLEAARESARLTLLAMLSSLRLALGSLDRVRDWVALSGF
                     VNADPSYAQTTAVLNPASDLLLEVFGEHGRHARSAIGVAALPLNLPVIMSAEVLVTP"
     gene            complement(14935..16179)
                     /locus_tag="VFC01_04240"
     CDS             complement(14935..16179)
                     /locus_tag="VFC01_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN78890.1"
                     /translation="MTSMASLSEIDFTDLDNFASGFPHSLFEVHRREAPVYWHSATEN
                     TPDGEGFWSVATYAETLAVFRDPATYSSVTGGSRPFGGTLLQDLAIAGQVLNMMDDPR
                     HSQIRRLVSSGLTPRMIRRVEDDLRARARRLLDSVEPGVPFDFLVDVAAELPMQMICI
                     LLGVPESERHWLFHAIEPQFDFGGSRKASVGQMSVEEAGSRMYAYGQELIASKRVSPT
                     DDMLSVVANATVDDDGQSGSLLSEIELYLFFSLLFSAGAETTRNSVAGGLLALAEHPD
                     QLAALRADLELLPTAVEEMVRWTSPSPSKRRTATRDVELGGCAIKAGQKVQIWEGSAN
                     RDALVFDRADEFDIARKPNPHLGFGQGVHYCLGANLARLELRVLFEELLSRLSSVRVV
                     KPVEWTRSNRHTGIRHLVVELR"
     gene            complement(16176..17594)
                     /gene="purB"
                     /locus_tag="VFC01_04245"
     CDS             complement(16176..17594)
                     /gene="purB"
                     /locus_tag="VFC01_04245"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZN78891.1"
                     /translation="MTIPNVLANRYASDEMVAIWSPEAKVVAERRLWLAVLSAQASLG
                     VDVPAGVVSDYEQVLDDVDLSSIAARERVLRHDVKARIEEFNALAGHEHVHKGMTSRD
                     LTENVEQLQIRRSLELVFTHGVAVVARLAEKAISYRDLAMAGRSHNVAAQATTLGKRF
                     ASAAEEMLLALTRLRTLIDRYPLRGIKGPMGTAQDMLDLFDGDVSKLAELERRVAEFL
                     GFTEIFTSVGQVYPRSLDHDVVSALVQVGAGPSSLAHTIRLMAGHELVTEGFAPGQVG
                     SSAMPHKMNTRSCERVNGLQVVLRGYGSMAAELAGAQWNEGDVFCSVVRRVALPDAFF
                     AIDGQIETFLTVLDEFGAYPAVIQRELDRYLPFLATTRILIAAVRAGMGREAAHEVIK
                     EHAVAVALAMREKGSEPDLLDRLAADPRLPLDRPALDAALADKQAFTGAAGDQVDRVV
                     DAVDELVGRYPEAAKYTSGAIL"
     gene            17636..18472
                     /locus_tag="VFC01_04250"
     CDS             17636..18472
                     /locus_tag="VFC01_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HZN78892.1"
                     /translation="MHFVGLDLAWGEKKQTGVAVVDADGRLLHVGAAQDNASIEAAIA
                     PFTAEDCLVAIDAPLIVKNQTGFRPCETAYNRDFQKFDAGARPAFRDNPAFNPPRAEV
                     LADLLGLDMDPSSTSSRRVIEVYPHPATVVLFKLQKILKYKRGEFEDRQRDLLQLMTH
                     IEGLDDASPRLRVNRNPAWVELHKRVQAATRPSQLDRDEDPIDAVMCAYVALLQYHRP
                     EDVTIYGDFETGYIVTPSLTDEVVPQRRGSRSSLENRLIRAMELLEDAQRELTAIREA
                     LR"
     gene            complement(18469..19844)
                     /locus_tag="VFC01_04255"
                     /pseudo
     CDS             complement(18469..19844)
                     /locus_tag="VFC01_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            complement(19905..20534)
                     /locus_tag="VFC01_04260"
     CDS             complement(19905..20534)
                     /locus_tag="VFC01_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN78893.1"
                     /translation="MLEVTAAVTPKGERRRYALVSAAADLLCEGGFDAVRHRAVARRA
                     GLPLASTTYYFSSLDDLIAKAVEHAGAREAEQLHARVAALSRRRRGAESTADVLVDLL
                     VGEANGLRVTEQLISRYERYIACARQPKLREIERSILQQRTDAVVEVVERSGRAVRAE
                     LLTALVCAVDGAVVAALVGDGDGPRATARATLIDVLDVLAPIDERVAPV"
     gene            20628..21518
                     /locus_tag="VFC01_04265"
     CDS             20628..21518
                     /locus_tag="VFC01_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016706122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN78894.1"
                     /translation="MMSRMPELNRRTLMRLGILTAAGAATTYAAGTALRTPNSSVAGP
                     SMAGVGAPLSPPAATAAPTYVNGSFLSAARGGVLTNWVIARPPGQTAPLRPVVLLHGK
                     DANVSGLMALGVEKFLADAVNAGVPPFAIAAADGGNSYWHKRASGEDSGAMVLDEFIP
                     MLESQGLDTSRVGFLGWSMGGYGAMLLGSRLGAGRTAAICAVSPAVWTSAGAAAGGAF
                     DGPDDYAANTVWGLPELNAIPLRIDCGTEDPFYEQTQQFIAQLPNTPAGGFSPGGHNM
                     DYWSAQLSPELSWIAPLLTA"
     gene            complement(21515..22783)
                     /gene="purD"
                     /locus_tag="VFC01_04270"
     CDS             complement(21515..22783)
                     /gene="purD"
                     /locus_tag="VFC01_04270"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZN78895.1"
                     /translation="MRVLVIGSGAREHALLLALRQDPELEELAVAPGNAGTAIIADQY
                     DVDVTSGEAVAQLAQRIRADLVVIGPEVPLVLGVADAVRAAGIACFGPSKDAARIEGS
                     KSFAKDVMTAAGVRTATSEIVDNPAHLDAALDRFGPPAGDEAWVVKDDGLAAGKGVVV
                     TADRDTARAHAASLLDAGHPVLLESFLDGPEVSLFSIVDGETVVPLLPAQDFKRVGDN
                     DTGPNTGGMGAYAPLPWLPDEVVSQIVNDIVKPVAAEMVRRGSPFSGLLYAGLAMTSK
                     GPAVVEFNCRFGDPETQSVLALLETPLGQLLRAAATGQLAEQPPLRWRDGYAVTVVVA
                     AENYPGRPRVGDVIVGAEADGVLHAGTARRDDGAIVSSGGRVLSVVGTGPDLVAARES
                     AYPLIKSIRLPGSHFRGDIGLAAAEGRISV"
     gene            complement(22802..23572)
                     /locus_tag="VFC01_04275"
     CDS             complement(22802..23572)
                     /locus_tag="VFC01_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase"
                     /protein_id="HZN78896.1"
                     /translation="MHTKKALVRRPCRRLAEGLLTHIERSAVDVDIAMKQWEGYVRAF
                     VDAGWRVTEVEPARDCPDSVFVEDTVVVYGDLAVIARSGAVERRPETAGTEKAVADLG
                     YRVAHIEAPGVLDGGDVLKHAGTVYVGQSGRTNADGIRQLTEYLAPFGATVVAVPLTK
                     ALHLKSAVTALPDGTIIGYEPVVDDPSVFPTFLPVPEEPGAHVVLLGDGKLLMSSAAP
                     ETAALMQARGYEPALVDIGEFEKLEGCVTCLSVRLRGI"
     gene            23632..24861
                     /locus_tag="VFC01_04280"
     CDS             23632..24861
                     /locus_tag="VFC01_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN78897.1"
                     /translation="MTQTTCPFGQGYDFTDPDVLLKGIPVTEFAELRKTAPVWWNEQV
                     DSIFSDGGYWVISRHADIKEISRNGDLWSTNRKGVVMRLPDGTTPDQLELTKALLINH
                     DAPEHTRLRKIVSRLFTPRAVATLEEKLAVAAREIVAAAKEKESGNFVDDIAMGLPLL
                     AIADLLGVPEADREKLFHWTNLIMNTDDPDFEADVIAANAELMGYAYNMAEERRRCPA
                     DDIVTRLIEADIDGEAIEDVEFAFFVILLAVAGNETTRNAMTHGMNAFFENPDQWELF
                     KRERPETTADEIVRWATPVHCFQRTALADTELGGVTVREGQRVGLFYSSANYDDAVFD
                     RPFEFNIARDPNPHLGFGGNGAHYCIGANLARMEIKLIFNEIANQLPDIAKLSEPQRL
                     RSGWINGVKELQVSYRG"
     gene            24871..25515
                     /locus_tag="VFC01_04285"
     CDS             24871..25515
                     /locus_tag="VFC01_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78898.1"
                     /translation="MRTHGWSGSAPASDEEAIARILDAASAAIDARGADFSIADVART
                     LGVTRQTVYRYFPSTDALLLASAQRAATDFLDRLTEHLRGVTDPVEAVAEGIATALEW
                     LPKDKHIGLLLMPGRASTFSQTVTSDVALQFANSILRRFDVDWAGLGFTDAELDELAE
                     HLLRIIQSFVIDPGRPPRSGNELRDYLRRWVGSAVTPPSVAATSAAGTRTSSRR"
     gene            complement(25454..26680)
                     /locus_tag="VFC01_04290"
     CDS             complement(25454..26680)
                     /locus_tag="VFC01_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78899.1"
                     /translation="MTSRYADLSREQLATLVPELLLIGQMIDRSGMAWCISNFGREEM
                     LQIAIEEWMGCSPIYTRRMQKALRYEGDDVITIFKGLQLDIGAPPQFMDFRYTVHDRW
                     HGEFHLDHCGALLDVEPMGEDYVRGMCHDIEDPTFDATAVATNRRAQIRPIHRPPRVP
                     ADRHPHCAWTVIIAESYPEVDDHPALDVIRRTRAARTELDPIDADDEGQGDYAGELLS
                     DFDFSAFSHSALVRMADEVCLQMHLLNLSYILAVGKRTGTNTQLATEICTKQLIGLAG
                     IAAERIHRALELPGGIEGAVRTLELHPMFNPVSYVTAEFGPDQVHVRRSPAHEDGAWI
                     SLVSPAETRPLQAAVATIDPHLDIELTGTETDWSARVIETDTAAKELPEVSVVRISGG
                     STFAFQPRKSLPLTVV"
     gene            complement(26670..27113)
                     /locus_tag="VFC01_04295"
     CDS             complement(26670..27113)
                     /locus_tag="VFC01_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN78900.1"
                     /translation="MDELRRKGLAKMNEVYAWEMPNMEGDEYFDLTVDHLFGTIWTRP
                     GLSMRDKRIMTLTVVTALGIQDLAEVQVNAALQNEELTEDELKEMAVFLTHYVGFPLG
                     SGLNGVVGRVVAKRKKAAAAGKGNDNKANVNDAVKMHTGKRLDDQ"
     gene            complement(27118..27972)
                     /locus_tag="VFC01_04300"
     CDS             complement(27118..27972)
                     /locus_tag="VFC01_04300"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN78901.1"
                     /translation="MSDNDVKLGYIGLGNMGAPMAKRLVEWPGGVTVFDVRTEAMTPL
                     AEAGATLADSVADVATADIISVTVLNDAQVRDVVGELAANAKPGTVIAIHSTISPETA
                     VDLAEQLAPQGIHIVDAPVSGGGGAAENGELAVMVGADREVYERIKPAFKQFASLVVH
                     AGPPGAGTRMKLARNMLTFISYAAAGEAMNLADQAGISLQQLGRVVRHTDAQSGGPGA
                     VIVRENSEMLDPDNFLYEPLAHARDLGEKDLTLALALGEAVGVELPMAEMALKNLAAG
                     LGVPRTKE"
     gene            complement(27969..28721)
                     /locus_tag="VFC01_04305"
     CDS             complement(27969..28721)
                     /locus_tag="VFC01_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN78902.1"
                     /translation="MGQFDEKVAIVTGAGGGIGQAYAEALAREGAAVVVADINTEGAE
                     RVAKQIVADGGTAIGVPVDVSDIDSAKAMADRTLAEFGGIDYLVNNAAIFGGMKLDLL
                     LTVDWDYYKKFMSVNLDGALVCTRAVYRKMAKRGGGAIVNQSSTAAWMYANFYGLAKV
                     GVNGLTQQLSRELGGMNIRINAIAPGPIDTEANRTTTPQEMVADIVKGIPLSRMGQPE
                     DLVGMCLFLLSDQAKWITGQIFNVDGGQIIRS"
     gene            complement(28731..30197)
                     /locus_tag="VFC01_04310"
     CDS             complement(28731..30197)
                     /locus_tag="VFC01_04310"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZN78903.1"
                     /translation="MGLRSDRQSQLFIDGKLVAGSGGTFPTVNPATEEVLGVAADATL
                     DDMGQAIDAARRAFDDTDWSTNTELRVRCVRQLQSVLREHVEELRELTIGEVGAPRML
                     TSMAQLEGPIADLSFCADTAESYEWTTDFGIAEPMGIPTRRTVVREAVGVVGAITPWN
                     FPHQINLAKLGPALAAGNTIVLKPAPDTPWCAAILGELIEHTDIPPGVLNIVTSSDHG
                     VGALLSKDPRVDMVSFTGSTATGRAVMADGAATLKKVFLELGGKSAFLVLDDADLTGA
                     CSMSAFTASMHAGQGCAITTRLVVPRARYDEAVEAAAATMSSLKPGDPNDAGTICGPL
                     ISERQRDRVQGYLDSATDEGGKFACGGGRPADRDTGFFIEPTVIAGLDNNAKVAREEI
                     FGPVLTVIAHDGDDDAVRIANDSPYGLSGTVFSADPERAAGIAARMRVGTVNVNGGVW
                     YSADMPFGGYKQSGIGREMGLAGFEEYLETKAIATAAN"
     gene            30338..>30475
                     /locus_tag="VFC01_04315"
     CDS             30338..>30475
                     /locus_tag="VFC01_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN78904.1"
                     /translation="MSSDAVVAITETPRNRRQEETFRKVLAAGVEMLRESSYADLTVR
                     AV"
BASE COUNT         5369 a        10380 c         9675 g         5051 t
ORIGIN      
        1 ccagcaccgt gtgcagcaac tcggactgcg cgccgtggtc cgactgcgag ccgacttcct
       61 cgtggtcgaa cagcaccagc actggcatgt gctcgccggg cgcggcggcc aagaacgcct
      121 ccagccccgc gtagcaggtc gcctggttgt ccagccgggg cgcgctcacc agatcctcgt
      181 tggctccggc cagcgtcgac ggtgtcaggt cgtgggtcat caggtcggcg cccaagacgt
      241 cggcttctgc gacccccgcg tgctcggcga cgtaacggag aaacgatcgg ctgccgctgc
      301 caacgcccca gaccgcgttg acgtgccgct gcggatccag tgagaccgac ttgcgatcct
      361 cggccaggtg gatggccaac tgcgggacgc gcaggatcgg ttcgtcgatg cgaacaagcc
      421 agtggtcaag tgtgttgccg acgcgtatcg acagccggcc gctgatgccg aggtcgcggt
      481 ccagccacga gttcagccag gcgccaccgt agggctgcag ggcgacgacc tgccagccgg
      541 agacgaaccg gtcggggtgc tgtttgaccc gcaggttggg gctgtcggtg tgggcgccga
      601 cgatgcggaa cggtccgctg cggtcgacct cgtttgcctg ccatgccacc agcgagccgg
      661 cccgcacggt gaagaatctg ccctgcgccg gccattcgtc gcgctcggag agttcggtga
      721 accccgcggc gctcaaccgc tgcgcgacag tcacgcagac gtggaacggc gacggcgacg
      781 cgtcgatgaa ctcgcacagc ccctgtgcac tcgcggccat ggttcaccat cattgctgac
      841 gcacgattcg ctagggtcaa acggtgcccg caccactacc gcaaccggta acggcgccgt
      901 tgacacccgc agccatcttc ctagtggtca ccatcgacga aggcggtgaa gccgccgtcc
      961 acgactcgct gcccgacatc tcggggctgg tccgcgcggt cggattccgt gactcggaca
     1021 agcgactgtc ggtgatcacc tcgatcgggt cggacgcctg ggaccgcttg ttctccggtc
     1081 cgcgaccggc cgagctgacg cggttcgtcg aactcgacgg cggacgtcac aaagccccgg
     1141 cgacgccagg ggatctgctg ttccacatcc gggcgatgtc gatggacatg tgctttgaat
     1201 tggcgggccg cctcgtcaag gcgatggggg ctgtgacggt cgtcgacgag actcacggtt
     1261 tccgtttctt cgaaaacagg gacctgctgg gcttcgtcga cggcaccgag aatccggccg
     1321 gcccacttgc ggtgtccgcc accgagattg gtgatgagga tcccgatttc gccggcggct
     1381 gctacgtgca cgtgcagaag tacctgcaca acatggcggc gtgggactcc ctgtcggtca
     1441 ccgagcagga actggtgatc ggccggacca agctggagga catcgagatg gatgacgatg
     1501 tcaagccggc gaattcgcac atcgcgctca acgtgatcga agacgaggac ggcaacgagt
     1561 tgaagatcgt ccggcacaac atgccgttcg gcgaaatcgg taaggacgaa ttcggtacgt
     1621 acttcatcgg ctactcgcgc acggccgcgg tgaccgaacg gatgctgcgc aacatgttca
     1681 tcggcgaccc gccgggcaat accgaccaca tcctggagtt ctcgaccgcg attaccggcg
     1741 ggaagttctt cacgccggtc gtcgactttc tcaatgatcc accgccgctt ccggattcgg
     1801 cgcccgaaga agagcaacca gtagtcaccg tcgcgcgcgt cgccaccaac gggtcgttgg
     1861 gcatcggcag cctgaaagga actccttcat gaacaacctg tatcgcgaac tggcgccgat
     1921 caccgaagcc gcgtgggacc aaatcgaaga ggaggcgacg cggacgttca agcggcacat
     1981 cgccgggcgg cgagtggtcg acgtcagcga gccgggcgga ccggtgaccg cggcgatcag
     2041 cacgggtcat ctgcgcgatg tcggggcgcc aggtgacggc gtggtggcgc atctgcgcga
     2101 gagcaagccc ctggtgcggt tgcgggtccc attcacggtg tcgcgcaccg ccatcgacga
     2161 cgtcgagcga ggctcacagg attcggactg ggatccggtc aaggacgccg ccaagaagct
     2221 ggcgttcgtc gaggaccgcg cgatcttcga gggctacgac gcggcgtcca tcgaaggcat
     2281 ccgggcgtgc agctccaatc cggcgctggc gttgcccgag gacgcccgct cgtatcctga
     2341 tgtcatcgcg caggcgctgt cggagctgcg gctcgccggt gtcgacggcc cgtattcggt
     2401 gctgctgtcg gccgacgact acacgaaggt cagcgagacc accgaacacg gctatccgat
     2461 ccgcgagcat ctcaatcgac tggtcgacgg cgacatcatc tgggcgccag ccatcgacgg
     2521 cgcgttcgtg ctgtctactc gcggcggcga tttcgatctg cagctgggca ccgacgtgtc
     2581 gatcggctac ctctcgcacg acgccgacaa tgtggagctg tatctcgagg agacgctgac
     2641 gttcctgtgc tacacggccg aggcgtcggt ggccttaacg ccctagcccc attacgccga
     2701 gcgtagttac ttaggcagcc agcaccagat ccagcgccga gtagaacagg cccagcccgt
     2761 cgtcggacgg gccggtcagc gcctcagtgg cgtgctcagg atgcggcatc aggccgacga
     2821 cgcggccgtt ggccgagctg atccccgcga tgtcgcgcat cgaaccgttg gggttgtcgc
     2881 ggtagcggaa caccacgcgg ccgtcgcctt cgagctggtc gagcaccttc tcgccggcga
     2941 cgtagcggcc ctcgccggac ttcagcggca ccagcaggtc ggcgccgagc tcgtagcgcg
     3001 acgtccacgc cgtcgaattc gacacgacct ccagccacag atcgcggcag acgaagtgca
     3061 gaccggcgtt gcgcgtcagc gcccccggca gcaggcccgc ctcacacaac acctgaaaac
     3121 cgttgcaaat ccccaacact ggcaggcctt gctcggcgcc ccgcaccacc tcgcccatca
     3181 ccggtgcgaa cttcgcgatc gcaccgcagc gcaagtaatc gccatacgag aagccgccgg
     3241 gcaccaccac cgcgtcgacg cccttgaggt cggcgtcggc atgccacagg ctgaccgcct
     3301 cggcgccggc cagccggacc gccctggcgg cgtcgacgtc atcgagcgtg ccggggaacg
     3361 tgatcacgcc cacacgtgcg gtcatgattg ctccctgttc accgaccagt cttctatgac
     3421 cgtattggcc aacagcgatt cggcgatttc ggcgagggtg tcgtcgtcga tactgtcatc
     3481 gacctcaagc tcaaaacgct tgccctgccg gacatctgag acaccgccga agccgagacg
     3541 gcccagtgcg ccgacgatcg cctggccctg cgggtcgagg atctccgcct tgggcatcac
     3601 gtgcacaacc acccttgcca cggcgatcac tctaccggcg gcaatgccgg gaccgtctca
     3661 ccggcacgac ccaaggtatg catcgcacag cggcgcggcc agcgcgtcga acacctgcgt
     3721 gtcggccacc gccggccagt cgacctgagg cgggagcgac gaccacaacg ccagttcgac
     3781 gacggtgctg ctggcggggt gcgccagcag gtactcgtgc atgacctgct cgcccgcagc
     3841 actgatcacc gccccgatgc gctggggatc gtcggtggtg atcgacggcg aggccacagc
     3901 ggccgtcgat tggcagtcgc ccagcctgga cctggcagtt tccaacgtgg taagcgccgt
     3961 gggtccaccg gtcgccgtgt cgccgcgcca gtggatcacc tgtacgagca ggttccattg
     4021 gccgtcctcg tgcagcacaa ccgaccgcgc tcccactgca tacgcacggg ggtcgctggc
     4081 aaccggcgga ctgccgcaca gcgcctcgaa gccaaaccgg ggcgacggcg ccgtgaccgc
     4141 caatcccgcc aggccgggcc accggtacac cgggtagagc gggacggacg tgggcgcgat
     4201 ccatgccgaa tcgggaatgg cgtcgcaaat cggcgggcac accccgggaa ccgcacgcgc
     4261 cggccagccg ccagtcggcg cgacggccaa cccgcaggtc gccatggcca cagccaagag
     4321 caatcgcacg gctcaagtac ccccttgcct ccccttgcgc cgccacaata tagggatgga
     4381 attgacgcat ttcggccatt catgcttact cgcaaatttc aagggcgatt ccggtcagga
     4441 aacaacggtg ttgttcgatc ccgggaactt ctcgcacgga ttcgagggca tcaccggcct
     4501 gtcggcgatc ctgatcaccc atcagcatcc cgaccacgcc gacaaggaac ggctcccggc
     4561 actggtcgac gcgaacccgc aggccgccct gtacgccgat ccccagacgg ccgagcagct
     4621 cggcggccca tggcaggccg tgcacgtcgg cgacgagttc agcgtcgggc acctgaccgt
     4681 gcgcggcgtc ggcgggcggc atgccgtgat ccacccggaa atccctgtga tcgacaacat
     4741 ttcgtatctc atcggcgacg gctcgcacgc ggcgcggttg atgcatcccg gcgacgcgct
     4801 gttcacgccc ggcgagccgg tcgacgtgct cgccacgcct gctgccgctc cttggatgaa
     4861 gatctcggag gccgtcgaat acctgcgagc ggtggcgcca gctcgtgcgg tgcccatcca
     4921 ccaggcgatc atcgaaccga acgcacgcgg catctactac ggccggctga aagagatgac
     4981 cgacaccgac tttcaggtgc tttcggagga aacccgtaca agcttctagg cggtttcgcc
     5041 tgccgcggcc cggcccgccg ccctcccgga gaacacgcag ccgccgagga acgtgccctc
     5101 cagcgaccgg tagccgtgca caccgccgcc accgaaaccg gcggcctcgc ccgcggcata
     5161 cagcccgccg aacacgctgc cgtcggcgcg cagcacccgc gagtcgaggt cggtctccaa
     5221 gccgcccaac gacttacgcg tcaggatgtg cagtttgacg gcgatcagcg gcccggcctt
     5281 cggatcggtg agccggtgcg gggcgacgac gcgaccgagc cggtcaccaa ggtagttgcg
     5341 cgccacgcga atcgacgcaa tctgggtgtc cttggtgaac cggttggcca cctcgcggtc
     5401 gcgcgcggtc acctcggcct cgacggtggc gtagtcgagc ggcagcacat cgggcacggc
     5461 attcatcgcg gacaccaagt cgcgcaacga gtttgcgctg acgaagtcga cacccttgtc
     5521 gacgaacgcc tgcaccggct ttggcgctcc ggcgcgcacc cgcgcaagca cgtcacgcac
     5581 gctcttgctg gtgaggtccg ggttctgttc ctgcccggac agcgcgaact ctttggcgat
     5641 gatccgcgcg ttgaggacat accaggtgta gtcctgtccg gtctgcgaaa tgtattccag
     5701 tgtgcccagc gtgtcgaagc cgggatacaa cggaggcggc agccgcttgc cgtgcgcgtc
     5761 cagccacagc gacgatgggc ctgggatgat ccggatgccg tgcagtggcc agatcgggtc
     5821 gtaattggtg atgccctcgg tgtagtgcca catccggtcg ctgttgatga cgtggccgcc
     5881 tgcggattcg gtgatgccga tcatccggcc gtcgacatgc gcgggcaccc cggacagcag
     5941 ctgttctggc acccgcccca tccgcgtggg ccagttctgc cggaccaggt cgtggttgcc
     6001 gccgatgccg ccgctggcga cgatcaccgc tgccgcgtga aattcgaagt cgccgacggt
     6061 gtttcgtgac gacgccacac cgcgcgccgc gctggtcggc tccagcaccg cgccacgtac
     6121 ccctgtgact gcgccgttct cgacgatcag ctcgtcgacg cgatggcggt gggcgaaccg
     6181 caccttcgcg ttacccagca gcttgcgcgc gaagacgtcg acgatcgccg ggcccgttcc
     6241 ccacgtgatg tggaaacgcg gcaccgagtt gccgtggccg cgtgcgtcgt agccgccgcg
     6301 ctcggcccag ccgacgaggg ggaaggtctg cagcccgcgc tcgcgcagcc agctgcgctt
     6361 ctcgcccgcg gcgaagtcga cataggcgtg tgcccactgc cgcggccagt ggtcctcggg
     6421 ccggtcgaag cccgcggcgc caagccagtc ctgcaacgcg agctcgtggc tatcgcggat
     6481 gcccagccgc cgctgttcgg ggctgtcgac gaagaacagg ccgccgaatg accaataggc
     6541 ctggccaccg acgttgttgg cgttctcctg gtccacgatc agcacgcggc ggccgcgctc
     6601 gaccagctca caggcggcga ccaacccggc cagaccggca cccaccacga tcacatcagc
     6661 gtcagccatg gccgccaatg tagcggcctc gccttaggcg gcgtctgtca gcgcgcggta
     6721 cggctcccag tggtagacgg acggcatgca gcgatgcatc aacgccagat cgacggcgtc
     6781 gagcatggcc tgtcgcggtc ccgaccggcg caggtgccgg gcggccactg cgacgcggac
     6841 gatgccctcg cggcgcgcgg tgcgctcggc cgacagcaca agcgtccggc accaccatgg
     6901 ctcgctcaag aacccctcgc cgatgccgag caccctgccc cgcacggttg tctgacgaac
     6961 caggtcggtg atgccgccta atcccgcgag tagccgaaag ccgttgcggg gcaaagcagt
     7021 caccgtgcct tgcgaaaccg tccaaccagg cgcggcgaac agccgggtac gcaacccaag
     7081 atgctcgagc acccggtcgg cggccatcag ccgcagattg gcctcgtgcg caggcaatgt
     7141 cgcgaactcg ccacgccgct tcttggtggc ggcctcgtcg aagccgtgaa gcacgatggc
     7201 gtcaccacgg ccgcgccgat cggccagcca gtcgacagtc ggcgcgtcgg agtcgagccg
     7261 gtagccgccc ttgatcctgg gcgccaccag aaacgaggca ggcacgtcgc gctcgtcgag
     7321 ctgacggcag aacgcctcaa cgtcggccag tgtgcggtcg ctgatcccgg agatcgagac
     7381 gatcagttgt ccagccacgt gttcagtctg gcaacgtcag gtgtcccaac ggtttacaac
     7441 acgtggtcag cagttgacca tctagcgcgg cagcaccgcc tcgatcgctg cgatcacctc
     7501 gggcgcgtcg ggaaccgtgc gcggccggaa tcggttgacc acttcgccac cgggtgccag
     7561 caggaacttc tcgaaattcc attgcacgtc gccggcttcg ccgctgacgt cgggcgtctg
     7621 ggtcagcttg gcgtacagcg ggtgccgatc cgcgccgttg acgtccgtct tggccagcag
     7681 cggaaacgtc acgccgtagt tcgtcgaaca gaacgtctga atctcctcgg cggtgcccgg
     7741 ttcctgtccc atgaactggt tgcacggcac gccgatcaca gtcaatccgc ggtcaccgta
     7801 ttccgtcgca agctgctcca gcgcggtgta ctgcggggtt aggccacatt tcgaggcgac
     7861 gttcaccaca agcgcggcgc cgtcggcgag ctcggccagc gtggtggggc gcccgtccag
     7921 cgttgtcagg ggaatgtcgg tcagcgaggt cattcccgca cattagccgc tcttgaagag
     7981 catccccgcg acgcggtgca ccatggccat cggatcgggg tcgttgtcga tggcctgatt
     8041 gagccacaac agcgaaaacc catgcaccag agaccaagcc gccagcgcgg cggcctcggg
     8101 gtcgcccttg gcgcgtacat catcgagggt gccgacgcct tgcgccaact cggtcccggc
     8161 cgcgccgacg gccgccacca actccgggtc gtccgggttg acgagcgagc gatcgaacat
     8221 caccgcgtag tggccgggat gggccagcgc gaaccgcaca taggccagcg ccgcatcgag
     8281 gaagtccggc cgggcgcccg atagtgcgtc ggccagcagc cgccacccct cggcggcgag
     8341 cgcggtgaac aggccgcggc ggtcggtgaa gtgatgcgca ggggcagcgt gcgacacgcc
     8401 agcctcccgc gccagttcgc gcagtgagat cccgtcggcg ccgcgctcgg ccaccagggt
     8461 ggcggccttg gccaggatga cggccttcag atcgccgtga tggtaggtgc ccctgctcat
     8521 ccggccatca tagcgaggaa tcttgacagt gcctagatcg ttggagtacg tttgcatctt
     8581 gacactggct agataaggag gttggcaatg gccgtcatcg tcacattggt cctgggcacg
     8641 gttggggccc gcttggtggg ctggctgggg gtcgactacg tcaacagctg gccgcaggct
     8701 gccgccgtcg ggctggcggc gatgttcgtg atgacagggg tcgcgcactt cgtgaacccg
     8761 cttcgccgcg acatgatcgc gatcgtgccg ccgcggctgc ccgcccccgg actgctggtc
     8821 acgaccactg gcgtgctgga actggtgggc gcggccggac tgctgtaccc gccgacccgc
     8881 gtcgcagcgg cggtgtgcct gttcgtgttg atgctggtga tgttcccggc caacatctac
     8941 gcgtcgcgga tgccgaaccc gccgaagtcg atgacctcgc ggctggacgt acgcaccgtc
     9001 gaggaagtcg tgtacctagc cgccgccgtc gtggttggcc ttggcagcat ccagtagcca
     9061 ggcgtactgg aaagccgctt ccttccaccg ttcgtagcgt ccgctgatgc caccgtggcc
     9121 ggcgttcatc tcggtcttca gcagcaccgg gtgatcgtcg gtcttggtgt gtcgaagcgc
     9181 cgcaacccat ttcgcgggtt cgacgtagag caccctggtg tcgttcaacg acgtcatcgc
     9241 gaggatcgcc ggatagtcct tggcttcgat gttctcgtag ggcgagtagg acttcatgta
     9301 gtggtagacg tccttgtcct ccagcgggtt tccccactcg tcccactcgg tcaccgtcag
     9361 cggcagcgac gggtcgagga tcgtcgtcaa cgcgtcgacg aacggcaccg cggccaggat
     9421 gccggcgaac aactgcgggg ccatattggc gaccgcgccc accagcagcc cgcccgcgct
     9481 tccgccgagc gcgacaaggt tgtctggtct cgtcacgtcg gtagcgatga gatggcttgc
     9541 gacggcgacg aagtcggtaa aggtgttgcg cttctccagc agcttgccgt gctcgtacca
     9601 tggccgaccc agctcgccgc cgccacgcac gtgagcgacg gcgaacacca tgccgcggtc
     9661 gagcagcgag agccgagcga tggaaaagcg tgggtcctcg cacgactcgt aggcgccgta
     9721 tccgtagagc aatgtgggcg cgggataggg cacgcctgtg cggtggacga tcgaaatcgg
     9781 cactcgcgca ccgtcggcgg cgacggccca gtcgcggcgc tcgacatact cgtcggggtg
     9841 gtagtcgccg agcaccggct gttcccgcag cagtgtgcgc tcgccggtgg ccaggtcgat
     9901 gtcgtagatg cgcaccggga tgacgaacga cgtcgcgccg atccgtagct tgggtgagct
     9961 ccaattgggg tttgcggaaa ggccggccga catcagttcg gagtcgaatg tgatgtcctc
    10021 ggggcggccg tactcgccgg tcgcgtatat cggccacagc tggatccgcg gcagcgcctc
    10081 gctgcggtag ctgaccacga gatggccttc gaacgcgtcg acggagtcga gtcgcacatc
    10141 gtcgcggtgc tcgatcagtg tgcgaaaagc ggtgggatca ctgaccggag cctcgaccaa
    10201 cgtgaagttc tcggcgccgt cgttgtgcat gatgaggaac cggtcctcac caccgacgac
    10261 cgcgtgttcg atggagtact cgacgagttc acggcgcggc cagatggcgg tgaattcggt
    10321 gtggccgtca gcggcgtcgc cgtagcgcaa ctcggtggtg acggcgctgc cggccgcaat
    10381 gacgatgtac ttgtcactgc gggtccggcc cactgcgagc cagaacttct cgtcgggctc
    10441 gtggtagacc cgttcggcgg gcagaccggc gccgaggcga tgccgccaca cggtgtcggg
    10501 gcgccaggcg tcgtcgacgg tgacgtagta gatggtgtga ttgtcggcgg cccaggtggc
    10561 gccggaaccg atgccgacta tctcgtcggc atacagggag ccggtgcgta aatccctgaa
    10621 ccgcaacgtg tatcgctcgt cgcccttgac gtccaccgaa taggccagga tgttgccgtc
    10681 caggctgacc gtggcggcgc cgagcgcgaa gaagtcgtgc ccttcggcct cgacgttctc
    10741 gtcaagcagg atctgctcac cgggtacttc ggtgtcctcg tcgaactgcg gcgggatcca
    10801 gtcgtcggga tcggcgacgg ggcaacggca ttgcacgctg tattgcttgc cctcgaagct
    10861 tcggccgtag taccaccagt cgccgcggcg gatcggcacc gagagatcgg tttccttggt
    10921 gcgcgccttt atctcgtcga agatcttctg ccgcaacggc gccaggtctg ccgtcgcgtg
    10981 ctccgtgtag tcgttctcgg cgttcaggta gccgaggact tcggggtcgg ccttgtcgcg
    11041 cagccactcg taggggtcga tgaacacatc accgtgatat tcccggcgag tgtcgatcct
    11101 cttggcttcg ggtggcttca cggcttgatc cagtcgctga acttcaggcc cgagatgcgt
    11161 tcgtaggctt cgatatagcg ggctcgggtg gcgtcgacga tgtcgggcgg cagcggtggc
    11221 ggcggcttgt cgccgttgcg gtcccagccg gactccgggc cggtgagcca attgcgcacg
    11281 aactgcttgt cgaagctctg ctggacgaca ccctcccggt agtcgtcggc ccgccagtac
    11341 ctgctggagt ccggggtgaa gacctcgtcg gccagccgca ggttgccgtg cttgtccaca
    11401 ccgaactcga acttggtgtc ggcgacgata acgcctttcg tcagtgcatg atcggcgccc
    11461 tggatgtagg tctgcagggt cttctcacgc aactggttgg ccctgacggc gccgaccatc
    11521 tcgatcacgt cgttgaacgt gatgttctca tcgtgttgtc cgagttcggc tttggtggcc
    11581 ggggtgaaca gcggctgggc gaacttgctg gcctcctcga gccctggcgg cagcgcattg
    11641 ccggatacct gaccagtctt ttggtagtcg atgaggcccg agccagtgag atagccgcgg
    11701 gccacgcatt ccaccggcat catctcgagc tcctgcacga ccagcgcccg cccgagcacc
    11761 tcttggggga tgcgctcgtc ctcgggtggg cccgccaagt ggttgggcgc ctcgacgtaa
    11821 tcgaagaaga agacgctcat cgcggtcagg atgcggccct tgtcgggtat ctcggagtcg
    11881 aggatgtagt cgtaggcgga gatccggtcg gtggcgacga acagcagggt ttcgtcgtcg
    11941 atgcggtaca gctcgcgaac cttgccgctg gccagatgtt cgtagtccga cagagacggg
    12001 cgcatcgggc cagcctatct gctgggatca acaccatgag gtcgcgttat ctgccctatg
    12061 ccaccacccc gacccgactg ctcgcgcagc tgatcagcga catggtggtg atcgcctgga
    12121 ccaccgtgtg ggtactggtc gggatggcgg tgcatgccgc cgtgtcgacc atcgccgaag
    12181 tcggccgtca ggtcgagacc ggtgccaacg gcgtcgcgga caatctgaac tcggccggcg
    12241 accgcacgga cgacttcccg ctcgtgggcg acgccttggc gaagcccttg aaagcggcca
    12301 gcgaggccgc gctcgacatc gcgggtgccg gacacagctt ggacaccacg gcgtcgtggc
    12361 tcgcgtgggt gctcgccctg gcggtggccg caccaccgat cctctttgtc gctctgccgt
    12421 ggctgttcct gcgtattcgg ttcttccgtc gcaagtggac cgccttgacg ttggcggcca
    12481 cgcccgccgg cgaacagttg ctggcgctgc gggcgctggc caaccggccg ctggccaagc
    12541 tcgcggcgat acacgccgat cctgtcggcg cctggcggag ccacgacgcc gccgcgatcc
    12601 acggcctggc cgcccttgag ctccgcgccg ccggagtccg ccggcgccac taacttgcga
    12661 tttgtgcacg atttcacgcg gtgtgagcgc ggattttcgt gcacaaatca ctcagagtgg
    12721 aaccggtggg gcgcgcgacg cgtccaccct aaatatggtc gacgagtatc tacgtcaaca
    12781 cgatggcgta attaccctcg ctcaagccat gcgcgctggc ctgagccatg atgccgtcaa
    12841 cagacgagtc cactcgggcc gatggctgcg ctgctcgccg ggcgtcttct ttgcagacga
    12901 tcggccgttt accgacgcat cgcgggtgcg tacagccgtc tggtcttgtg gcccaaaggc
    12961 aactgcgagc ggtctggccg ccgcgtggtg gcacggtgtc actcgtttcg ctccagagac
    13021 tgtcgaggtc accgtggcca aagtgagcaa tcatcgacga cgacctggcg tccgcactag
    13081 acgacgtgac ttggcacccg cagacatcgt cgagcggaag ggcctaaggg tgacggcgtt
    13141 gccgttgacg gttatcgaag ccgctgtacg acggggcggc ggccccaagc tgatggattc
    13201 agcgttgcag cgccatgtcg agctgccgca gctttggaaa gcacacctgc gaaatagggg
    13261 tcgccacggg tcgcccgcgg cgcgacgact actgcaggcc gcatccgacg gcgcgcgttc
    13321 ggaagccgag cgcctgttgg tcaagctgtt gcgcgacaac aacatcaccg gctggaagac
    13381 caactatccc gtcggcggat acaaggtcga cgtcgcattt cctgagcaga aggtcgccat
    13441 cgaaacagac gggtgggctt accacagcag ccaagaagac tttcaaaacg atcgcgtacg
    13501 gcagaacaag atcacgctgc tcggctggca ggttcttcgc tttacgtggc tagatctagt
    13561 cgagtatccc cagcgggtgc tggcggagat ccgattcgcg agagcgcatc cagcgcggta
    13621 agcatccacg ccacgaacgc gatccagaag aacgccagcg acactgtcgt cagccaatgc
    13681 caacctgttt cgacagccgt cgcatacgtc gccgacgagt acatcccgag tgggaacacc
    13741 gtcggccatg cgatgcccac gcgtcgtcgc agcgtgacgt aaaccagcgg caggatcaac
    13801 gccgtcgcaa caatccacgt cacgaccgtc accggccaca cgctcgtcag cccggccttg
    13861 tggatgtgat cgccggccag cgtcgcgacc gccgcaccgc ccatcagaat ccatatgtcg
    13921 ggctgcgcca actccggcgc agacgaatca ccgacagcac gccacacgac cagtccagtc
    13981 atcaccaggt agatacagat cgcaagggcc caaagaatca ctgcccagaa gacgatcttc
    14041 aggtccgcca ttacgatggc caatccggat gtcgcgacgc tcgccagttc ccagccgccg
    14101 cgcgcacggt cacgcaagcc tgcgcggtcg cgccacatcc ggcgcgccac caccggcgcc
    14161 agcgagagcc agccttgcaa tgccatgccc gccagtaccc atagcacgat tcggtgttcg
    14221 gccagccgcg ccgccaccac cgcacaggcc gcgacgtatg agcacaaccg aagcgacaca
    14281 tcgagatcgg tgagtttcca cgactctcgt cgccaggcga tggccgccgc cacgatcagc
    14341 accggcaacg cgacggcagc caacacgatc gacacgtcgc tgatgacccg atagccgtga
    14401 tccatcgccg cgatcgacac gatgccggtc gccatgaccg ccgcgaacgt atcgggtttc
    14461 acggcgtgac aagcacctcc gccgacatga tcacgggcag gttcagcggt agcgccgcca
    14521 cgccgatcgc gctccgcgcg tgtctgccgt gctcgccgaa cacctcgagc agcagatccg
    14581 acgccgggtt gagcaccgcg gtcgtctgcg cataactcgg gtcggcgttg acgaaaccgc
    14641 tcagtgccac ccaatcacgc acgcgatcaa gcgatcccaa cgccaaccgc aaagagctaa
    14701 gcatcgccaa cagcgtcagt cgcgcggact cccgcgccgc ctccaacggc acctcgccag
    14761 gcactcgtcc gaacggtcca caaggcgcac cgtccgagcc aagcgcacca tgcccactca
    14821 acacgcaccg ctcaccgacc acacgcgccc attcgaacgg aatgcgaacg cccggcggta
    14881 acaacggctc cggcggcaac tgcaggccga gttcggagac acgctgctca aaagtcaccg
    14941 cagttcgacc accaggtgac gaatgccggt gtgacggttg ctgcgcgtcc actccacggg
    15001 tttgaccacc cgcaccgacg agagccggct aagtagctcc tcgaacagca cccgcagctc
    15061 gagccgcgcc aggttggcgc ccagacagta gtgcacgccc tgaccaaaac ccaagtgcgg
    15121 attcggcttt cgcgcgatgt cgaactcgtc ggccctatcg aaaaccaagg cgtcgcggtt
    15181 ggccgacccc tcccaaatct gtaccttctg tccggccttg atcgcacatc cgcccagctc
    15241 gacgtcgcgc gtggcggtgc gtcgctttga cggcgacggc gacgtccacc gcaccatctc
    15301 ctcgacagcc gtcggcaaca gctccaaatc cgcacgcagc gcagccaact gatcgggatg
    15361 ttcggccaag gccagcagcc cgcccgcgac tgagttacgg gtcgtttcag cgcccgcact
    15421 aaacagcagg ctgaagaaga ggtacagctc aatttccgaa aggagggacc cagactgacc
    15481 atcgtcgtcg acggtggcgt tcgcgacgac cgacagcatg tcgtcggtcg gcgacacacg
    15541 cttcgacgcg atcagctcct ggccgtacgc gtacatccgc gaaccggcct cctcgacgga
    15601 catctgccca acggaagctt tacgcgaccc gccgaagtcg aactggggct cgatcgcgtg
    15661 gaagagccaa tgacgttcgg actccggcac tcccaacagt atgcagatca tctgcatcgg
    15721 cagttcggcg gcgacatcga ccaaaaagtc gaacggcacg cccggctcaa cggaatccaa
    15781 caaccgccgc gcccgcgccc gcagatcgtc ttcaacacga cggatcatcc gcggcgtcaa
    15841 cccggaactg accaaccggc ggatctgcga gtgccgcggg tcgtccatca tgttcaagac
    15901 ttggcccgcg atggccaaat cctgcagcag agttccgccg aacggccggg agccgcccgt
    15961 caccgacgaa taagtagccg gatcacgaaa caccgcaagg gtttccgcat acgtcgcgac
    16021 ggaccagaag ccctccccat cgggcgtgtt ctccgtcgcc gagtgccagt acaccggcgc
    16081 ctcacgccga tgcacctcga aaagcgaatg tgggaagccg gaagcgaagt tgtccagatc
    16141 ggtgaagtcg atctcggaca acgacgccat cgaagtcaca ggatcgcgcc cgaggtgtac
    16201 ttggccgcct cgggataacg gcccaccaac tcgtcgaccg cgtcgaccac cctgtcgacc
    16261 tgatcgccgg cggcaccggt aaacgcttgc ttgtcggcca acgcggcgtc gagcgccggc
    16321 cggtccagcg gcagccgcgg gtcggcggcc agccggtcga gcaggtcggg ctccgaaccc
    16381 ttttcgcgca tcgccaacgc caccgcgacg gcgtgctcct tgatcacctc atgcgcggct
    16441 tcgcggccca tgcctgcgcg caccgcggcg atcaggatcc gtgtggtggc caggaacggc
    16501 agatagcggt ccagttcgcg ttgaattacc gccggatagg cgccgaactc gtcgagcacc
    16561 gtcaagaacg tctcgatctg cccgtcgatc gcgaagaacg cgtcgggcag cgcaacccgg
    16621 cgcaccaccg agcagaacac gtcgccctcg ttccattgcg cacccgccag ctccgcggcc
    16681 atcgacccgt agccgcgcag cacgacctgc agcccgttca cccgctcgca tgaccgggta
    16741 ttcatcttgt gcggcatggc cgacgagccg acctgccctg gcgcgaagcc ctcggtcacc
    16801 agttcatggc cggccatcag ccggatcgtg tgtgccagcg acgacgggcc agcccccacc
    16861 tgcaccaacg cggagacgac atcgtgatcc agcgaccgcg ggtagacctg accgacggac
    16921 gtgaaaatct ctgtgaagcc gaggaattcg gcgacgcgcc gctccagctc cgccaacttc
    16981 gaaacatcgc cgtcgaacag gtcgagcatg tcctgcgcgg tgcccatcgg gcccttgatg
    17041 ccgcgcaacg ggtatcggtc gatcaacgta cgcagccgcg tcaacgcaag aagcatctcc
    17101 tcggcggccg acgcgaaccg cttacccaaa gtcgttgcct gcgcggcaac gttgtgacta
    17161 cggcccgcca tcgccaggtc gcggtagctg atggccttct cagccagtcg ggccaccacc
    17221 gcgacaccgt gcgtgaagac caattcgaga gaccggcgga tctgcagctg ctcaacgttt
    17281 tcagtgaggt cgcggctcgt catgcccttg tgcacatgtt cgtggcccgc caaagcgttg
    17341 aactcctcga tacgcgcctt gacgtcgtga cgcagcacgc gttcccgcgc cgcgatggac
    17401 gacaaatcga cgtcgtccag cacctgctca tagtcggaga ccacgccggc agggacgtcg
    17461 acgcccaacg aggcctgcgc cgagagcacc gccagccaca agcggcgctc ggcaacgacc
    17521 ttggcctccg gcgaccagat ggccaccatc tcgtcgctgg cataccgatt ggccagcaca
    17581 ttagggatgg tcacagatac acagcttacg gcgcgggtca ggcacagtag gggccatgca
    17641 ctttgttggg cttgaccttg cgtggggaga aaagaagcag acgggtgtcg cggtggtcga
    17701 tgccgacgga cggctcctgc acgtcggcgc cgcgcaggac aacgcgagca tcgaggccgc
    17761 catcgccccc ttcacggccg aggactgtct ggtggccatc gatgcgccgc tgatagtcaa
    17821 aaaccagact ggattccggc cttgtgagac cgcctataac cgcgatttcc agaaatttga
    17881 cgcgggcgca cggccggcct tccgggacaa ccccgccttc aatccgcccc gggcggaggt
    17941 gctggcggat ctgctcggtc tcgacatgga tccgtcctcc acatcgagca ggcgcgtcat
    18001 cgaggtgtac ccgcatccgg ccaccgtggt ccttttcaag ctccagaaga tcctgaagta
    18061 caagcggggc gagttcgagg atcgccaacg cgacctgctg caattgatga cgcacatcga
    18121 gggccttgac gacgcgtctc cgcggctgcg ggtaaaccgc aatccggcgt gggtcgagtt
    18181 acacaaacgg gtgcaagccg ccacccgacc aagccagctc gatcgagacg aggatcccat
    18241 cgacgccgtg atgtgcgcat acgtcgcgct tctccagtat caccggcccg aggacgtaac
    18301 gatctacggc gacttcgaaa ccggctacat cgtcacgccg tcgctgaccg acgaggtcgt
    18361 cccacaacgc cgcggcagcc gttcctcact cgagaaccgg ctgatccgcg cgatggaact
    18421 actcgaagac gcccaacgtg aactgacagc gatccgtgag gcgctccgct aatccggcgc
    18481 cgaacccaga tgcgtcgggt ccgtgatacc tgcgtcgcgg ataccgagct gccggttgat
    18541 cgccatggtg aggaagaaca gcagaattcc gatgccgacg agaatcagcg ccaacacata
    18601 ctgctgggcc ggccggcccg acaacggcgt caccaaatac agcgacgcca cacagccgat
    18661 caccggcagc acggtcgggg tcttgaaatg cccgccggcc tcctgaacgt cgcgccgcag
    18721 caccagcacc gcgacgttga ccatggcgaa caccgcaagc aacaacaagg acgtggtgcc
    18781 acccagcacg gcgatcgcgt tgctgttggc gaacgctgtg acgtagaaga tcaacccgaa
    18841 cgcgatcagc gtggtgaaca cgatcgccac ccatggcgtg agccgcctcg gatgcaccgc
    18901 gcccagcacc ggaggcagca cgtgctggcg ggacatgccg tagatcagcc ggcttgccat
    18961 caacatgttg atcagggccg tattggacac cgcgaacatc gagatgaacg gcaggatgtc
    19021 ttcgatcggc agccccggcg caccggcctc gaccacctcg accaacgggg tgtcgctggc
    19081 ctccactacc ccgactggca cgagcgccac cgcgacgatc gccacgatca catagacgac
    19141 accggcgatc gaaagacccg tcaacaagac cttcggaaac acccgaaccg gatccttggt
    19201 ctcctcggcc atgttcaccg aatcctcgaa gccgaccatg gcgaagaagg ccagcgacgt
    19261 cgcggtcgtg acggcgagga atatgttctt gtcggaggcg gtttcgaacg cgacgacgcg
    19321 ggaaaagtcg acgtcgccac caccggtgaa cgcccacagg ccgacgagta tcaccagcag
    19381 caggccgctg atctcgataa tggtcagcgc cacattgagt ttcacgctct caccgacgcc
    19441 gcgcaggttg acggtcgcga gcatcgccat gaacagcagc gccacgatga caattccgac
    19501 cttgccccaa tcgaggtcga acgccgtgtt gaagttcgcc gcgaagaacc gcgacgcggt
    19561 ggacgccgac gtgatgcctg aacacatcac cacgaatgcc accaggaacg tcacgaacgg
    19621 cacaccaaaa gccttgtgtg cgtacagcgc ggcacccgcc gcctgcgggt acttggtgac
    19681 cagctccagg tagctgaacg ccgttacggt ggcgatcagg aagcaacgag gaacggaatc
    19741 cacgccgccc caccgacttc ggcggccaca tccccagtca acgcgtatac gccggtgccg
    19801 aggatgtcgc cgacgacgaa cagcagcagc agtccggggc ccatgacccg ccgcagttgt
    19861 ggttgttccc caacggtgtc cggccagtca tcgccctctt ccgatcagac cggtgccact
    19921 ctctcgtcaa tcggcgcaag cacgtcgagc acatcgatca acgtcgcgcg ggccgtcgcc
    19981 cgcggaccgt caccatcgcc gaccagcgcc gccaccaccg cgccgtcgac ggcgcacacc
    20041 agcgcggtga gcagttcggc acgcaccgcg cggcccgacc gctcgacgac ctcgaccacc
    20101 gcatcggtgc gctgctgcaa aatgcttcgc tcgatctccc gtagctttgg ctgccgcgcg
    20161 caggcgatgt accgctcgta gcgcgagatc aattgctcag tgacacgcag tccgttggcc
    20221 tcaccgacca acaggtccac caacacatcg gccgtcgact ccgcgccgcg acgccgacgc
    20281 gacagcgcgg ccacccgcgc gtgcagttgt tcggcctcgc gcgctcccgc atgttcgacg
    20341 gccttggcga tcaggtcgtc aagcgacgag aagtaatagg tcgtcgacgc gagcggcagc
    20401 cccgcgcggc gggcgacggc gcggtgccgg acggcgtcga acccgccttc gcacagcaga
    20461 tcagcggccg cgctgaccag cgcgtaccgc cgacgttctc ccttgggagt gaccgctgcc
    20521 gtcacctcta gcatgctgcc agtcagcggg ttgcggcata gcgctttcgg ctaatcatca
    20581 ggctttactc agggaagtcc ggcgaaggcc ggccagcggc caatggcatg atgtcccgca
    20641 tgcccgaatt gaaccgccgc acgcttatgc gtctcggcat ccttacggca gcgggggcgg
    20701 ccactaccta tgcggcgggc accgcgcttc ggactcccaa ctcgtccgtc gctggcccgt
    20761 ccatggcggg cgtcggcgcg ccgttgtctc cgcccgcggc caccgccgca ccgacgtacg
    20821 tcaacggatc gttcctatcg gccgcccgcg gcggcgtgct gacaaattgg gtgatcgccc
    20881 gcccgcccgg ccagactgca ccgctgcgtc ccgtcgtctt gttgcatggc aaggatgcca
    20941 acgtgtcggg tctgatggcc ctgggcgtgg agaagtttct ggccgacgcg gtgaacgccg
    21001 gtgtgccccc attcgcgatt gcggccgccg acggcggcaa tagctactgg cacaagcggg
    21061 catccggcga agactccggc gcgatggtgc tcgacgagtt catcccgatg ctcgagtcgc
    21121 agggtctgga cacctcgcga gttgggttcc tgggttggtc gatgggcggt tacggcgcaa
    21181 tgctgctcgg ctcgcgcttg ggcgcgggcc gaacggccgc gatctgcgcg gtgagcccag
    21241 cggtgtggac gtcggccggc gctgccgcag gcggggcctt cgacgggccc gacgactacg
    21301 cggccaacac cgtctggggg ctgcccgagc tgaacgccat cccgctccgg atcgactgcg
    21361 gcacagagga tccgttctat gagcagacgc agcagttcat cgcgcagctg ccgaatacgc
    21421 ccgcgggcgg gttttcaccc ggcgggcaca acatggacta ctggtccgca cagctgagcc
    21481 ccgagctgag ctggatagcg ccgctgctga cagcttagac gctgatgcgg ccctcggccg
    21541 ccgcgaggcc gatgtcgccg cgaaaatggc tgcccggcaa gcgaatcgac ttgatcagcg
    21601 gataggcgga ctcccgcgcc gccaccaggt cggggcccgt tccgacgacc gacagcaccc
    21661 ggccacccga cgacacgatc gcaccgtcat cgcgtcgtgc ggtgccggcg tgcagcacgc
    21721 cgtcggcctc ggcgccgacg atcacgtcgc ccacccgcgg ccgtcccgga tagttctcgg
    21781 cggccaccac caccgtcacg gcgtacccgt cgcgccaacg cagcggcggc tgctcggcca
    21841 actggcccgt cgcggccgcg cgcagcagtt gcccgagcgg ggtttccagc agcgccagca
    21901 ccgactgcgt ctccggatcg ccgaagcggc agttgaattc gaccaccgca ggccccttcg
    21961 acgtcatcgc cagtccggcg taaagcagac cagagaatgg gctgccacgc cgaaccatct
    22021 ccgccgcaac gggtttcacg atgtcattga cgatctgcga gaccacctcg tcgggtagcc
    22081 acggcagcgg tgcgtaggcg cccatgccgc cggtgttggg gccggtgtcg ttatcgccga
    22141 cccgcttgaa gtcctgcgcg ggcagcagcg gcaccaccgt ctcgccgtcg acgatgctga
    22201 atagcgacac ctcggggccg tcgagaaacg attcgagcag caccggatgg cccgcgtcga
    22261 gcaagctggc ggcatgcgcg cgggcggtgt cgcggtcggc ggtcaccacc acgcccttgc
    22321 ccgcggccag tccgtcgtcc ttgaccaccc aggcctcgtc gcctgccgga ggcccgaacc
    22381 ggtcgagcgc tgcgtcgagg tgcgccgggt tgtcgacgat ctcgctggtc gcggtcctca
    22441 ccccggcggc cgtcatcaca tccttggcga acgacttcga tccttcgatg cgcgcggcgt
    22501 ctttcgatgg gccgaagcaa gcgatgcccg cggcgcgcac cgcgtcggcg acaccgagga
    22561 ccagtggcac ctcgggcccg ataaccacca ggtcggcgcg gatgcgctga gccaactgcg
    22621 cgacggcctc gccggacgtg acgtcgacgt cgtactgatc ggcgatgatc gcagtccccg
    22681 cgttgcccgg cgcgacggcc agctcctcca actcagggtc ttggcgcagc gccagcagca
    22741 acgcatgttc acgggctccg gatccgatca cgaggacgcg cacaggctca ccctaatggg
    22801 ctcagatgcc gcgaaggcga accgaaaggc acgtcacgca tccctcgagc ttttcgaact
    22861 cgccgatgtc gacgagcgcc ggttcatacc cgcgtgcctg catcagtgcg gcggtctccg
    22921 gagcggccga ggacatcagc agctttccat cgccgagcag aacaacgtgg gcgcccggct
    22981 cctccggtac cggaaggaat gtcgggaaga ccgacgggtc gtccaccacc ggctcgtagc
    23041 cgatgatggt gccgtccggt agcgcggtca ccgcggactt gaggtggagc gccttggtca
    23101 gcgggaccgc aaccaccgtc gcgccgaacg gcgccaagta ctcggtcaac tggcggatcc
    23161 cgtcggcatt ggtgcgcccg gactgtccca cgtagaccgt tccggcgtgc ttgaggacgt
    23221 ctcccccgtc gagcacgccg ggcgcctcga tgtgcgcgac gcgataaccg agatcggcga
    23281 ccgctttctc ggtgcccgca gtttccggcc ttcgttccac agcaccagaa cgcgcgatga
    23341 cggcgaggtc gccgtacaca acgacggtgt cctccacgaa cacgctgtcg ggacagtcgc
    23401 gtgcgggctc gacctccgtc acccgccagc cggcatcgac aaacgctcgg acgtagccct
    23461 cccactgctt catcgcaatg tccacgtcga cggccgatcg ttcgatgtgg gtgagaagcc
    23521 cctcggccag gcggcggcac ggccggcgca ccaaggcctt tttggtgtgc acggtccgat
    23581 cgtagggctt aactcggcca tacacttgac accacagtgt atggtcaaag tatgacgcag
    23641 accacatgcc ccttcggcca gggctatgac ttcaccgacc ccgacgtgct gctcaagggc
    23701 attcccgtca ccgagttcgc cgagctgcgc aagaccgcgc cggtgtggtg gaacgagcag
    23761 gtcgattcga tcttctccga cggcggctac tgggtgatca gtcgtcacgc cgacatcaag
    23821 gagatctcgc gcaacggcga cctgtggtcc accaaccgca agggtgtggt gatgcgactg
    23881 cccgacggca ccacccccga ccagctcgag ctgaccaagg ccctgctgat caaccacgac
    23941 gcaccggagc acacccggct gcgcaagatc gtgtcgcggc tcttcacgcc gagggcggtc
    24001 gccacgctgg aggagaagct ggcggtcgcc gcccgcgaaa tcgttgccgc tgcaaaggaa
    24061 aaggagtcag gcaacttcgt cgacgacatc gcgatgggcc tgccgctgct ggcgatcgcc
    24121 gatctgctcg gggtgcccga ggcggatcgt gaaaagttgt tccactggac caacttgatc
    24181 atgaacaccg acgatccgga cttcgaggcg gacgttatcg ccgccaatgc cgaattgatg
    24241 ggttacgcct acaacatggc cgaggaacgg cgccggtgtc cggcagatga catcgtcacc
    24301 cggctgatcg aggctgacat cgacggcgag gccattgagg atgtcgagtt cgcgttcttc
    24361 gtgatcctgc tcgccgtggc gggcaatgag accacccgca acgccatgac gcacggcatg
    24421 aacgcgttct tcgagaaccc cgaccagtgg gagctgttca agcgtgagcg gccggagacg
    24481 acggccgacg agatcgtccg ctgggccacg ccggtgcact gctttcagcg aacggcgttg
    24541 gcggacacgg agctcggtgg cgtcacggtt cgcgaaggcc agcgcgtcgg cctgttctac
    24601 agttcggcca actacgacga cgcagtcttc gaccggccgt tcgaattcaa catcgcgcgc
    24661 gacccgaacc cgcatctcgg cttcggcggc aacggggcgc actactgcat cggcgccaac
    24721 ctggcccgca tggagatcaa gctgatcttc aacgagatcg ccaaccagct gcccgatatc
    24781 gccaaactgt ccgaacctca acggcttcgg tccgggtgga tcaacggggt caaggagctt
    24841 caggtctcct atcgcggata gcatccaggc gtgcgtactc acggctggtc gggctcggcc
    24901 cccgcaagcg acgaggaggc catagcccgg atcctcgatg cggcgtcggc ggccatcgat
    24961 gcacgcggcg ccgacttctc cattgccgac gtggcacgca cgttgggcgt tacccgccag
    25021 acggtgtacc ggtatttccc cagcacggac gcgctgctgc tcgcatccgc tcaacgcgcg
    25081 gcgacggatt tcctggaccg gttgaccgag catctgcgtg gcgtcacaga tcccgtcgaa
    25141 gccgtcgccg agggcattgc cacggcgctg gagtggctgc cgaaggacaa gcacatcggc
    25201 ctgctcctga tgccgggtcg cgccagcacg ttcagccaga cggtgacctc ggatgtcgcg
    25261 ctacagttcg cgaactcgat tctgcggcgc ttcgatgtcg actgggccgg cctaggcttc
    25321 accgacgccg aactcgacga actcgcggag cacctattgc ggatcatcca gtcgttcgtc
    25381 atcgaccctg gccggcctcc gcgcagcggc aacgaactgc gggattacct acgacggtgg
    25441 gttggctcgg ccgtcacacc accgtcagtg gcagcgactt ccgcggctgg aacgcgaacg
    25501 tcgagccgcc gctgatcctc accaccgaca cttccggcag ttcctttgcc gccgtgtcgg
    25561 tttcgatcac acgcgccgac caatcggtct cggtaccagt gagctcgatg tcgaggtgcg
    25621 gatcgatggt ggcgacggcc gcctgcagcg gacgggtctc ggccgggctg accaacgaga
    25681 tccaagcgcc gtcctcgtgc gcgggtgacc ggcgcacatg cacttggtcg ggcccgaact
    25741 cggcagtcac atacgacaca gggttgaaca tcgggtgcag ttccagcgtg cggaccgcgc
    25801 cctcaatccc acccggcagc tccagcgcgc ggtgaatgcg ttcggcggcg atgcccgcca
    25861 ggccgatgag ctgtttggtg cagatctccg tggccagttg cgtgttcgtt cccgtccgct
    25921 tgccgaccgc gaggatgtac gacaggttca gcaggtgcat ctgcaggcac acttcgtcgg
    25981 ccattcgcac cagtgccgag tgtgagaatg ccgagaaatc aaaatctgag agcagttcgc
    26041 ccgcatagtc gccctggccc tcatcgtcag cgtcgatcgg gtcgagttcg gttctggccg
    26101 cgcgggttcg gcggatgacg tccagcgcag gatggtcatc cacctctgga tacgactcgg
    26161 cgatgatcac ggtccacgcg caatgcgggt gacggtcggc cggcacccgg ggcggccggt
    26221 gaatcggccg gatctgcgct ctgcggttgg tggctaccgc ggtggcgtcg aaggtcgggt
    26281 cctcgatgtc gtggcacatg ccgcgcacgt agtcctcacc catcggctcg acatcgagca
    26341 gcgcgccgca gtggtcgagg tggaactcgc cgtgccagcg gtcatgcacg gtgtagcgga
    26401 agtccatgaa ctgcggcggg gcaccgatgt cgagctgcag gcccttgaag atcgtgatga
    26461 cgtcgtcgcc ctcatagcgc agcgccttct gcatccgcct cgtgtagatc gggctgcagc
    26521 ccatccactc ctcgatcgcg atctgcagca tctcttcgcg gccgaaattg ctgatgcacc
    26581 aggccattcc ggaccggtcg atcatctgcc cgatcaacag aagctcgggt accagcgtgg
    26641 cgagctgttc gcgcgagagg tcggcgtacc tactggtcat cgagcctctt acccgtatgc
    26701 atcttgaccg cgtcgttgac attggccttg ttgtcgttgc ccttgcccgc cgcggcagcc
    26761 tttttgcgct tggcgacgac gcgcccgaca acgccgttga ggcccgaacc gagcgggaag
    26821 ccgacgtaat gggtgaggaa taccgccatc tccttcaact cgtcctcggt caactcctcg
    26881 ttctgaagcg ctgcgttgac ctgaacctcg gcgaggtctt gaattccgag cgcggtcacc
    26941 actgtgaggg tcatgatccg cttgtcccgc atcgagagtc cgggcctggt ccagatggtg
    27001 ccgaagagat ggtcgacggt caggtcgaag tactcgtcac cttccatgtt cggcatctcc
    27061 cacgcataga cctcgttcat cttggcgagg cccttgcgac gcagttcgtc cattgtgcta
    27121 ctcctttgtg cgcggcacgc cgagccccgc cgccaggttt ttcagcgcca tttcggccat
    27181 cggcagctcg acacctacgg cttcgcccag ggcgagcgcc agggtcaggt ccttctcgcc
    27241 gaggtcgcga gcgtgggcca gcggttcgta gagaaagttg tcagggtcca acatctccga
    27301 attctctcgg acgatgacgg caccgggccc gccgctctgg gcatcggtgt gccgcaccac
    27361 ccgccccagc tgctggagac tgattcctgc ctggtcggct aggttcatcg cctcaccggc
    27421 ggcggcgtag gagatgaaag tcaacatgtt gcgcgccaac ttcattcgcg tacccgcgcc
    27481 cggagggccg gcgtggacca ccaacgacgc aaactgcttg aacgccggct tgatgcgctc
    27541 gtacacctcg cggtcggcac ccaccatcac ggccagctcg ccattctcgg cggccccgcc
    27601 gccgccgctg accggggcgt cgacgatgtg gatgccttgc ggtgcaagct gttcggcgag
    27661 gtccaccgcg gtctcggggc tgatggtcga atggatcgcg atgacggttc ccggctttgc
    27721 gttggccgcg agttcgccca ctacatcgcg cacctgcgca tcgttgagca ctgtgacgct
    27781 gatgatgtcg gcggtcgcga cgtcggcgac actgtcggcc agcgtggcac cggcttcggc
    27841 cagcggcgtc atcgcctcgg tgcgcacgtc gaaaaccgtg actccgcccg gccattcgac
    27901 cagccgcttg gccatcggcg cgcccatatt gccgaggccg atgtagccga gcttgacgtc
    27961 gttgtcactc atgatctgat gatctgccct ccgtcgacgt tgaagatctg accggtgatc
    28021 cacttggcct gatccgacag caggaacagg cacataccga cgagatcctc tggttgtccc
    28081 atgcgggaca acggaattcc cttgacgatg tcagcaacca tctcctgcgg cgtcgtcgtc
    28141 cggttcgcct cggtgtcgat cggcccaggt gcgatcgcgt tgatccggat attcatccca
    28201 cccagctcac gcgacagctg ctgcgtcagg ccgttgacac cgaccttggc cagaccgtag
    28261 aagttcgcgt acatccacgc cgcagtcgat gactggttga caatcgcgcc accgccgcgc
    28321 ttggccatct tgcggtacac cgcgcgggtg cacaccagcg cgccatcgag gttcacgctc
    28381 atgaacttct tgtagtaatc ccagtccact gtcagcagca gatccagctt catgccgccg
    28441 aagatcgcgg cgttgttgac cagataatcg atgccgccga attcggccag cgtgcggtcg
    28501 gccatcgcct tggccgagtc gatgtcggaa acgtccaccg gcacgccgat cgccgttccc
    28561 ccgtcggcga cgatctgctt ggccactcgc tcggcgcctt cggtgttgat gtcggccacc
    28621 acgaccgcgg cgccctcgcg ggccaatgcc tcggcgtagg cctggccgat gccgccaccg
    28681 gccccggtga cgatcgcgac cttctcgtcg aactgcccca tcaagtctcc ttaattcgct
    28741 gctgtcgcaa tggctttggt ttccagatac tcctcgaaac cggcgagccc catctcccgg
    28801 ccgatgccgg actgcttgta tccgccgaac ggcatgtcgg ccgagtacca gacaccgccg
    28861 ttgacgttga cggtgccgac ccgcatccgc gcggcgatcc ccgcggcccg ctcgggatcg
    28921 gccgagaaca ccgtgcccga cagcccgtac ggcgagtcgt tggcgatgcg gacggcgtcg
    28981 tcgtcgccgt cgtgtgcgat caccgtcagc accgggccga agatctcctc gcgggcgacc
    29041 ttggcgttgt tgtcaaggcc cgcaatgacg gtcggctcga tgaagaaccc ggtatcacga
    29101 tctgcgggac gtccaccgcc gcacgcgaac ttgccgccct cgtcggtggc cgagtccagg
    29161 tagccctgta cacggtcgcg ctggcgctcc gaaatcagcg gtccgcatat tgtgcccgcg
    29221 tcgttcgggt cgccgggctt cagcgaggac atcgtcgctg ccgccgcctc gacggcttcg
    29281 tcgtagcggg cacggggcac caccagtcgg gtggtgatcg cgcagccctg cccggcgtgc
    29341 atcgaggcag tgaacgcgga catcgagcac gcgcccgtga gatccgcgtc gtcgagcacg
    29401 aggaatgccg acttaccccc cagttcgagg aagaccttct tcagcgtggc ggccccgtca
    29461 gccatcaccg cgcgccccgt cgccgtcgac ccggtgaacg aaaccatgtc cacccgtggg
    29521 tctttcgaca acaatgcacc cacgccgtgg tcgctggagg tgacgatgtt tagcactccc
    29581 ggcgggatgt cggtgtgctc gatgagttcg ccgaggattg ccgcgcacca cggggtgtcc
    29641 ggcgcgggct tgagaacgat ggtgttgccc gccgccagcg ccgggccgag cttcgcaagg
    29701 ttgatctggt gcgggaagtt ccacggggta atagcaccca ctacgccgac ggcttcacga
    29761 accaccgtgc ggcgcgtggg aatccccata ggctcagcga tgccgaaatc ggtggtccac
    29821 tcatacgact cggcggtgtc cgcacagaaa ctcaggtcgg cgatcgggcc ttccagctgc
    29881 gccatcgatg tgagcatccg cggcgcgccg acctccccga tggtgagctc acgcagttct
    29941 tcgacgtgct cgcgcagcac ggactgtagc tggcgaacgc agcggacccg cagttcggtg
    30001 ttcgtcgacc agtcggtgtc gtcgaacgct cggcgggccg catcgatcgc ctggcccatg
    30061 tcgtcgagcg tcgcgtcggc ggcgacaccc agcacctcct cagtggccgg gttgaccgtc
    30121 gggaacgtgc cgccactacc ggccaccagc ttgccgtcga tgaacagttg actctggcga
    30181 tcgctgcgca ggcccatacc aactctccgc ttctttgctg gacaactgtc cgacttggct
    30241 caaatcgaac gatagccctc gatggctccg cctggcaagc agaccctctt tacctgcatg
    30301 cttgcctata acccaccgcg tcggataact tggacatgtg tccagcgatg ccgtggtcgc
    30361 catcacagaa acaccacgta accgccgcca ggaggaaacc ttccgcaagg tgctcgccgc
    30421 aggcgtggag atgctgcgcg agtcgtcgta cgccgacctg accgtgcgcg cagtg
//
LOCUS       DASRJA010000094         6184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_200529,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000094 DASRJA010000000
VERSION     DASRJA010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200529"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFC01_04320"
     CDS             complement(<1..666)
                     /locus_tag="VFC01_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HZN78905.1"
                     /translation="MVTAQAPTQVSSAEMDVVSGPRVGVKYAVEAIGTFFLVFTVGAA
                     VGSGSPLAPLGIGAVLMVMIYAGGHLSGGHYNPAVTMAVLIRRRIGLRDAVGYWIVQL
                     GAGLLAAVVVRTVVDPTRMATAAATTLTGRTLLAVFVVELLFTFALCYVVLNVATSKD
                     HPDNSFYGLAIGFTVVAGAFAVGAISGGAFNPAVSLGAAVMGMFAWPTLWIYLVAQVL
                     GGIA"
     gene            complement(772..1806)
                     /locus_tag="VFC01_04325"
     CDS             complement(772..1806)
                     /locus_tag="VFC01_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN78906.1"
                     /translation="MTAREAFGAEFVGADGFLNSPTYGLPPRFLIDALQDCITQWQTG
                     TMEVPLFDDYVRFGRVGYATLTGVPVESVAMGGNVSAVLGLVAAAIPDGSRVATLAGE
                     FTSTTFPFAAQAGRGVTVTELAPDQLVSDAADFDVVTASLVQSANGAVLDIDALRNTV
                     AGTDTLTVVDITQALGWKQVDLGWVDVTAAAVYKWLLAPRGTSWMSLSDRVSRLMTPH
                     AANWYAGEDPWQTIYGLPLRLSDSARRFDASPTWFSALGAGLTLPWLAALDGAAVEAH
                     TVGLANRTRTELGLPQQNSAIVSIPIADAADRLRAAGIRASVRAGAVRIGFHLYNTED
                     DLDRLLDALG"
     gene            complement(1803..2111)
                     /locus_tag="VFC01_04330"
     CDS             complement(1803..2111)
                     /locus_tag="VFC01_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4229 domain-containing protein"
                     /protein_id="HZN78907.1"
                     /translation="MSDRPPRSRLVIDVLAYVLARLLLVVVLTAVIFGAGHLLGVRDF
                     PLVVALLFAFVVALPLGIWIFAPLRRRATASIAVFDERRRKDREQLQARLRGEEPPAE
                     "
     gene            2162..2323
                     /locus_tag="VFC01_04335"
     CDS             2162..2323
                     /locus_tag="VFC01_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78908.1"
                     /translation="MAYLLLTLVLAGLIYAGWRLTRLTAAARPKTRVIGPDDDPDFLR
                     RLGDNKPRQ"
     gene            complement(2327..3436)
                     /locus_tag="VFC01_04340"
     CDS             complement(2327..3436)
                     /locus_tag="VFC01_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HZN78909.1"
                     /translation="MSEQSDGFRSQQRFSDPAEAVAAEAVPAEWTAPTPPNGLPLGYA
                     LRPLTPQPEDPPPYLDLSTVALLGQPKRAPSQGWRKWLYLASFKQINVGESAKVNRHN
                     SLVNEVKRPLRGCYRIALLSLKGGVGKTTITATLGATFASTRGDRVVAVDANPDRGTL
                     SQKVPLETPATVRHLLRDAEGIAAYSDVRGYTSQGPSRLEVLASESDPAVSEAFSSED
                     YQRTLEVLERFYSLVLTDCGTGMLHSAMSAVLDKADVLIVISSGSVDGARSASATLDW
                     LDAHGHQDMVRNSIAVINAVRPRSGKVDMKKVVDHFNRRCRAVKQVPFDPHLEEGAEI
                     SLDRLKPETREALIELAAVVSAGFSGAKRPQAQSG"
     gene            complement(3473..4447)
                     /gene="ccsB"
                     /locus_tag="VFC01_04345"
     CDS             complement(3473..4447)
                     /gene="ccsB"
                     /locus_tag="VFC01_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HZN78910.1"
                     /translation="MNTEHVDIGLARYSDWAFTSSVVVLVAALLLLAVELAYSRGRKA
                     EARELVGATVGADSATPGVVADTPKRPLGERAGKAGLALVYVGIGLLLVCIVLRGLAT
                     ARVPWGNMYEFINLTCLSGLVAAAIVLRKPQYRALWVFVLVPVLILLTVSGRWLYTNA
                     APVMPALQSYWLPIHVSVVSLGSGVFLVAGVASILFLLQMSPLGQPGPDSAVRRIVHR
                     LPDAQTLDRIAYRTTIFAFPVFGFGVIFGAIWAEEAWGRYWGWDPKETVSFIAWVVYA
                     AYLHARSTAGWRDKKAAWINVVGFVAMVFNLFFINLVTVGLHSYAGVS"
     gene            complement(4521..6071)
                     /locus_tag="VFC01_04350"
     CDS             complement(4521..6071)
                     /locus_tag="VFC01_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HZN78911.1"
                     /translation="MRSTWRTLTSMGTALVLLFLLALGAIPGALLPQRSLNEFKVEQY
                     IAEHPTIGPWLDRLQAFEVFSSFWFTAIYVLLFISLVGCLTPRLIEHARSLRATPVAA
                     PRNLSRLPKHHAAEVPGDPETVAADVSDHLRGWRRISRKEGATVEISAEKGYLREFGN
                     IVFHFSLLGLLVAVAAGKLFGYEGNVIVIADKGPGFCSASPAAFDSFRAGNTVDGTSL
                     YPICLRVNDFQAHYLPTGQAVSFAANIDYQAGDDLNTDSWRPYHLKVNEPLRVGGDRV
                     YLQGHGYAPTFTVTFPDGQTRTQTLQFRPEDQTTFLSSGAMRFDPPGGSYPDPDERRK
                     NQIAIQGLFAPTEQLDGTLLSSSFPALNNPAVAVDIYKGDTGLDTGKPQSLFNLDPGL
                     IEQKRLTKKARVNLRAGQETRLDDGTIVRFDGAVPFINVQVSHDPAQIWVLVFALTMM
                     AGLLVSLVVRRRRVWVRLTPAGPGTVSVELGGLARTDNSGWGDEFERLKQRLLDVGVQ
                     REAEAHRV"
BASE COUNT         1083 a         2098 c         2020 g          983 t
ORIGIN      
        1 ggctattcca ccaagtacct gggccaccag gtatatccac agcgtcggcc acgcgaacat
       61 gcccatcacc gccgccccaa gcgagaccgc cgggttgaac gcgccgcctg agatcgcccc
      121 gaccgcgaat gcgcctgcca cgacggtgaa tccgatcgca aggccataga acgagttgtc
      181 ggggtggtcc ttgctggtgg ccacattgag cacgacgtag cacaaggcga acgtgaagag
      241 cagctccacc acgaacacag ccagcagggt gcggccggtc agcgtcgtcg ccgctgcggt
      301 cgccatccgt gtcgggtcga ccaccgtgcg caccaccacc gcggcaagca gccccgcacc
      361 gagctgcacg atccagtacc caaccgcatc acgcagaccg atccggcgcc gaatcaacac
      421 cgccatcgtc acggccgggt tgtagtgccc accggagagg tgtccgcctg catagatcat
      481 caccatcagg acggccccga ttccgagcgg cgccaacggg ctgccgctgc ccaccgcggc
      541 gcccacagtg aagaccagga agaaggttcc gatcgcctcg accgcgtact tgacgccgac
      601 ccgaggcccg ctcacgacgt ccatctctgc gctgctcacc tgagtcggtg cttgtgctgt
      661 caccatggtt catagactcg cggtcccgga cgccgatgtc gtcaacgctg tcggccatct
      721 cggccccccg gcagcgcgac tccgggccta cctgctagct gctattgccc gtcaacccag
      781 agcgtcgagc agccggtcca gatcgtcttc ggtgttgtac aggtggaagc cgatgcgcac
      841 cgcccctgca cgcacggaag cgcgaatacc tgcagcccgc agcctgtcag ccgcatcagc
      901 gatcggtatc gacacgatgg ccgaattctg ctggggcaga ccgagttctg tgcgtgtccg
      961 gttggccagc ccgacggtgt gcgcttcgac cgcggcaccg tcaagcgccg ccagccacgg
     1021 cagcgtcaac ccggcgccca atgcgctgaa ccatgttggt gaggcgtcga aacgtcgcgc
     1081 gctgtcggac agccgcagcg gcaacccgta gatcgtctgc cacgggtcct cacccgcgta
     1141 ccaattggcg gcgtgcggtg tcatgagccg gctcaccctg tcgctcaagg acatccacga
     1201 ggttccgcgc ggcgccagca gccacttgta gaccgcggcg gccgtgacgt ctacccagcc
     1261 caggtcgacc tgtttccagc cgagcgcctg cgtgatgtcg acgacggtca gcgtgtcggt
     1321 gcccgcgacg gtgtttcgca gcgcgtcgat gtcgagcacg gcgccgttgg cggactgcac
     1381 caggctagcc gtcacgacgt cgaagtcggc cgcgtccgag accaactgat cgggcgccag
     1441 ctcggtgacc gtcacgcccc tgccggcctg ggcggcgaac gggaaggtgg tgctggtgaa
     1501 ctcgcctgcc agcgtcgcca ccctgctgcc gtcgggaatc gccgcggcaa ccagcccgag
     1561 gaccgcagat acgttgccgc ccatggccac cgactcgacg ggcacccctg tcagcgtggc
     1621 gtagccgact cgcccgaagc gcacgtagtc gtcaaacaac ggcacttcca tcgtccctgt
     1681 ctgccactgg gtgatgcagt cctgcagcgc gtcgatcagg aatcgcggcg gcaagccgta
     1741 ggtcgggcta ttgagaaacc cgtcggcgcc gacgaattcg gcgccgaacg cctccctggc
     1801 cgtcattcgg cgggtggctc ttcgccgcgc agccgggcct gcaattgctc tcggtccttg
     1861 cgtcggcgtt cgtcgaagac ggcgatgctg gcggtggcgc gacggcgcag aggggcgaat
     1921 atccagatgc ccagcgggag tgcgacgacg aacgcgaaca agagcgccac cacaagcgga
     1981 aaatcgcgca cgccgagcaa atgtccggcg ccgaagatca ccgcggtcaa gacgacgacg
     2041 agcagcaggc gggccaggac ataggccagc acgtctatca cgaggcggga tcggggcggt
     2101 cggtcagaca cgggccgagc ctaccgacgg cgcgtatatt catttcaagg aggttttttg
     2161 cgtggcgtat ttgctcctga cactcgtctt ggccggattg atctacgccg gctggcggct
     2221 gacgcggttg accgccgccg cccgaccgaa gacgcgcgtg atcggaccgg acgacgatcc
     2281 ggatttcctg cggcggctcg gggacaacaa gccccggcaa taggtcctat ccggactggg
     2341 cctgcggccg cttcgcaccc gagaagccag ccgagacgac ggcggccaat tcgatcaacg
     2401 cctcccgtgt ctccggcttc agccggtcga ggctgatctc ggcgccctcc tccagatgcg
     2461 ggtcaaaagg cacctgtttg acagcgcggc accgccggtt gaagtgatcg accaccttct
     2521 tcatgtcgac cttgccggac ctcggccgca cggcgttgat cacggcgatc gagttgcgaa
     2581 ccatgtcctg gtggccgtgg gcgtccagcc aatccaaggt ggccgacgcg ctgcgggcgc
     2641 cgtcgacgga gcctgagctg atcacgatga gcacgtcggc cttgtccagt acggccgaca
     2701 tcgccgagtg cagcatgccg gtgccgcagt cggtgagcac caggctgtaa aaccgctcca
     2761 gcacctcgag cgtgcgctgg tagtcctcgg agctgaacgc ctccgacacc gccggatcgc
     2821 tttcggatgc gagcacctcg agccggctcg ggccctgtga ggtgtagcca cggacgtcgc
     2881 tgtaggccgc aatgccctcg gcgtcacgca gcagatgccg cacggtggcc ggggtttcca
     2941 gcggcacctt ctggctcagc gtgccgcggt cgggattcgc atcgacggcg acgacgcggt
     3001 caccacgggt cgacgcgaac gtcgcgccaa gtgtcgcggt gatcgtcgtc ttgccgacgc
     3061 cgcccttcaa cgacagcaac gcgatgcggt agcagccccg cagcggtcgc ttgacctcgt
     3121 tgaccaggct gttgtgccga ttgaccttcg cgctttctcc gacattgatc tgcttgaacg
     3181 acgcgagata cagccacttg cgccagcctt gcgacggcgc tcgcttgggc tgacccagca
     3241 gcgcgacggt cgacaggtca aggtacggcg gcgggtcttc gggctgcggc gtcagcgggc
     3301 gcaacgcgta gcccagcggc aagccgttcg gcggggtcgg tgccgtccat tccgcgggta
     3361 ctgcctccgc ggctacggcc tctgcggggt cgctgaaccg ctgctgcgac cgaaacccat
     3421 ccgactgctc agacaacgac taagtcccct ctcaactagc accttgtcgc ggtcaactca
     3481 ccccagcgta cgaatgcagc ccgacggtca ccagattgat gaagaacagg ttgaatacca
     3541 tcgcgacgaa gccgacgacg ttgatccagg ccgccttctt gtcgcgccac cccgccgtcg
     3601 agcgcgcgtg caggtacgcc gcgtagacca cccacgcgat gaacgacacc gtctccttgg
     3661 ggtcccaacc ccagtagcgg ccccacgctt cctcggccca gatcgcgccg aagatgacgc
     3721 cgaacccgaa caccgggaac gcgaaaatgg ttgtgcggta agcgatgcgg tccagcgtct
     3781 gcgcgtccgg cagtctatgc acgatgcggc gcacggcgct gtccgggccc ggctggccga
     3841 gcggcgacat ctgcagcagg aacaggatgc tcgcgacacc cgccaccagg aagacgcccg
     3901 agccgaggct gaccaccgac acgtggatgg gcagccagta cgactgcagg gctggcatga
     3961 cgggtgcggc gttggtgtag agccaacgcc ccgacacggt cagcaggatc agcaccggaa
     4021 cgagcacgaa cacccacagc gcgcggtact gcggcttgcg caacacgatc gcggcggcaa
     4081 ccagacccga caaacaggtg aggttgatga actcgtacat gttgccccac ggcacccgcg
     4141 ccgtggccag gccgcgcagc acgatgcaca ccagcagcag cccgatgccg acgtagacca
     4201 gggccagccc ggccttgcct gcacgctcgc cgagcgggcg cttcggcgta tcggcgacga
     4261 cgccgggggt ggcgctatcc gcgccgacag tcgctcccac gagctcgcgc gcttcggcct
     4321 tgcggccgcg gctgtacgcc agctcgacgg cgagcagcag cagcgcggcc accagcacca
     4381 ccaccgacga ggtgaaggcc caatcggagt agcgggccaa cccgatgtcg acgtgctcgg
     4441 tgttcatgcc tcgactcccg ctcgctccgg tcctcgctcg tccctcgctg cgatcctccc
     4501 tcgcgctcgt cttcgggtgt tcatacgcga tgtgcctccg cctctcgctg gacgccgacg
     4561 tcaagtagac gctgcttcaa ccgctcgaac tcgtcgcccc atcccgagtt atcggtgcgc
     4621 gccaagccgc ccagctcgac gcttaccgta cctggacctg cgggtgtcag ccgaacccag
     4681 accctgcgcc gccgcaccac cagcgacacc agcaggccgg ccatcatggt cagcgcaaac
     4741 accagcaccc agatctgggc ggggtcgtgg gagacctgca cgttgatgaa tggcaccgcg
     4801 ccgtcgaacc ggacgatggt gccgtcgtcg agccgggtct cctgaccggc acgcaggttg
     4861 accctggcct tcttggtgag acgcttctgc tcgatcagcc ccgggtcgag gttgaacagt
     4921 gactgtggtt tgccggtgtc cagcccggtg tcgcccttgt agatgtcgac ggcgacggcc
     4981 ggattgttca gcgccggaaa gctcgacgac agcagcgtgc cgtcgagctg ttcggtgggt
     5041 gcgaacaggc cctggatggc gatctggttc ttgcgacgct catcgggatc gggatagctg
     5101 ccgccgggcg ggtcgaatcg cattgcaccc gacgacagaa acgtggtctg gtcttcgggc
     5161 cggaactgca gcgtctgcgt gcgggtctgc ccgtcgggga aggtgacggt gaacgtcggg
     5221 gcgtagccgt ggccctgcag gtagacccgg tcgccgccga cccgcagcgg ctcgttgacc
     5281 ttcaggtgat acggccgcca gctatcggtg ttgaggtcgt cgcctgcctg gtagtcgatg
     5341 ttggcggcga agcttacggc ctgaccggtc gggaggtagt gcgcctggaa gtcgttgacc
     5401 cgcaagcaga tcggatacag cgacgtcccg tcgacggtgt tgccggcccg gaacgagtcg
     5461 aacgccgcgg gcgaggctga gcagaacccc gggcccttgt ccgcgatgac gatgacattg
     5521 ccctcgtagc caaagagctt gccggcggcc acggcgacga gcaggccgag cagcgagaag
     5581 tgaaagacga tgttgccgaa ttcgcgcaga tagcctttct cggctgatat ttcgacggtg
     5641 gcgccttctt tgcggctgat ccgacgccag ccgcgcagat ggtcggacac gtcggcggcg
     5701 acggtctcgg ggtcgccggg aacctcggcg gcatggtgct tgggcagcct gctcaggttg
     5761 cggggtgcgg cgacgggcgt ggcgcgcagg ctgcgggcgt gctcgatcag tcgcggagtc
     5821 aggcagccga cgagcgagat gaacagcagc acatagatgg ccgtgaacca gaagctggag
     5881 aacacctcga aggcctgcag ccggtccagc catggtccga tggtcggatg ctcggcgatg
     5941 tactgctcga ccttgaattc gttgaggctg cgttgcggca gcagcgcacc cgggatggcg
     6001 cccagcgcga gcagaaacag caacaccagc gcggtgccca tcgacgtcag ggtccgccag
     6061 gtgctgcgca tcagcgcgag cagacgcaaa actgccctat tttgacggat tttgggcagt
     6121 tttgcgtctg ctcgcgggga aacgttcgtc atatcggcag cgtcacgtcg ctgacgaaca
     6181 cgtc
//
LOCUS       DASRJA010000095         4574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3623801,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000095 DASRJA010000000
VERSION     DASRJA010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3623801"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFC01_04355"
     CDS             complement(<1..365)
                     /locus_tag="VFC01_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZN78912.1"
                     /translation="MDATDAVALADLDAIGQAALVASGRLSATELLEAAIVRLEAGRE
                     LNAVIADLFDHGRQQAAALDASGVLRSGGSGPLAGVPFLLKDLGASLAGAPEAMGSRA
                     LRTHVATDSAWIVDRYLAAG"
     gene            475..753
                     /locus_tag="VFC01_04360"
     CDS             475..753
                     /locus_tag="VFC01_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HZN78913.1"
                     /translation="MTESLNRQFTARLQKSDNKGGWTYVVMPDSAAFFGTRGLVKVRG
                     TVDGQPFASSFMALGDGTHKLPVKTDLRRRIGKEAGGEVTVHLEERIS"
     gene            874..1455
                     /locus_tag="VFC01_04365"
     CDS             874..1455
                     /locus_tag="VFC01_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN78914.1"
                     /translation="MTRLADFTMVTVRGPETRATTIDCPPDGEWAAIRFRLGVHMPSL
                     PTRLLLDHRDVHLAVSADGSFELAGSRWPLPDLDNAERYVHELARRGVIARDDVVDAA
                     IGGDGSALTIRSVQRHFRRTTGMTHSLFRQIERARRATTLLRAGAPILDTVHEAGYFD
                     QAHLSRSLRVFIGETPASVMRNDRQLSFLYKTG"
     gene            1480..1854
                     /locus_tag="VFC01_04370"
     CDS             1480..1854
                     /locus_tag="VFC01_04370"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN78915.1"
                     /translation="MRVEPYVYFQGRCEEALEFYRAALGADATVLARFGDKVGHAEVR
                     IGDTVLLASDGQGDGPSTFSGFSLSLSASDETEADRLFAALSDGGSVQVPIAPTPFAS
                     RLGLVADKFGVPWMVVSQNAAG"
     gene            complement(1834..2073)
                     /locus_tag="VFC01_04375"
     CDS             complement(1834..2073)
                     /locus_tag="VFC01_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78916.1"
                     /translation="MEPHHLKAPDLVPVEALRSGEHVTDVNGGGQHYIVLESKQLSGD
                     CVVLELESKANDQLRVIEMSFPAGYQMGRSPRRIL"
     gene            complement(2127..2483)
                     /locus_tag="VFC01_04380"
     CDS             complement(2127..2483)
                     /locus_tag="VFC01_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78917.1"
                     /translation="MGHASYPPPKPPIAGADLAISISALILTVIFGGAAAVMGLFSLA
                     FLDYCPPQSCSAEGAATAVGTGLLAAFAIGVVGLVLTVIQLYRRKPGWPFAVATFVLC
                     AVAVGLGGVGDAMAVG"
     gene            2574..4205
                     /locus_tag="VFC01_04385"
     CDS             2574..4205
                     /locus_tag="VFC01_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HZN78918.1"
                     /translation="MHSTMQDFPLTVTGILRHGTGWYSSRRVTTATPGGYREISYGEL
                     GTRVAQLAHGLRQLGVTDGDRVATFMWNNQEHLETYFAAPCMGAVLHTLNVRLAGDQI
                     AFIANQAEDRVVLVDMSLAPLLAPVLPEMTSVQTVVAVGEGDVAQLNTSGKNVIRYDD
                     LLDGQPLEYSWPDLDERSAAAMCYTSGTTGNPKGVVYSHRSSYLHSMSVCAANALGLA
                     DGDRVLPIVPMFHANAWGQPYAAMMAGADLLLPDRFLQAAPLVDMIEQRRPTIAAAVP
                     TIWNDVLQYLHANPGRDVSSLRLVACGGSAVPLGMMKEYEQSHSVHILQGWGMTETSP
                     LAALAAPPPEVTGSDHWALRSTAGRVLCGVEVRLVDDAGAVLPNDGKAVGEIEIRGPW
                     ITGSYYQNADPSKFDNGWLRTGDVGRIDPQGFITLTDRAKDVIKSGGEWISSVELELL
                     IMDHPAVLEAAVVAVPDDRWQERPLAAVVVKNGATVAAPELRKHLCDKVARFWLPERW
                     TFIDEVPKTSVGKFDKKLIRSRYADGVYDVLECRD"
     gene            complement(4216..>4574)
                     /locus_tag="VFC01_04390"
     CDS             complement(4216..>4574)
                     /locus_tag="VFC01_04390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZN78919.1"
                     /translation="SGRDGYVADTARVARALGVPDPPLTQKELRERIEEFRPELSGTA
                     AAREAARFLLLTPPLPIAARAPYGLLAATSVSMLPAWARMPLLLPYFPPVEATVIRVA
                     GRVVVGGIRWALTAGR"
BASE COUNT          803 a         1496 c         1517 g          758 t
ORIGIN      
        1 cccgcggcga gataccgatc gacgatccac gccgagtcgg tcgcgacgtg ggtgcgcagc
       61 gcgcgtgagc ccatcgcctc gggcgcaccg gcgagcgagg cgccaagatc cttgagcaag
      121 aacggaactc ccgcgagcgg acctgaccca ccgctgcgca acacaccgga ggcgtcgagc
      181 gccgcagcct gctgtcggcc atgatcgaaa aggtcggcga tgacggcgtt cagttcccgg
      241 ccggcctcga ggcgcacaat cgcggcctcc agcagttcgg tcgccgacag ccgaccggaa
      301 gcgaccagcg ccgcctgccc gatggcgtcg agatcggcga gggccacggc atcggttgcg
      361 tccatgggga cggatcattc agcgccgtca tgcgcgcggc aacttgagcg tccgcgatgt
      421 cttttcggac gccgccgagt cgttatagat gtacggccac cgaaaggacg aatcatgacc
      481 gaatccttga accggcagtt caccgcgaga ctgcagaaga gtgacaacaa aggcggatgg
      541 acctacgtcg taatgcccga ttcggcggcc ttcttcggca cccgcggcct ggtcaaggtc
      601 cgcggcaccg tggacggtca gccgtttgca agctcgttca tggcgctcgg cgacggcaca
      661 cacaaactgc ccgtgaagac cgacctgcgt cggcgcatcg ggaaagaggc cggcggcgag
      721 gtgaccgtgc acctcgagga acgcatcagc tgaatgttcc ttcgctttgc agaccgggca
      781 tcggactcgc cgtacatcga acgggtgtgg cgggcccata gcgatggcgg cggcccgttc
      841 gtctccgtcg ccgccggtca tctcgagctg gtggtgacgc ggctcgccga cttcaccatg
      901 gtcaccgtgc gcgggccgga gacccgggcc accaccatcg actgcccacc agacggcgag
      961 tgggcggcga tccggttccg gctcggtgta cacatgccgt cgctgccgac ccgcctgcta
     1021 ctcgatcatc gcgacgtgca cctggccgtg tcggccgacg gatcgttcga gctcgcgggc
     1081 tcacgatggc cgcttccgga cctggataac gccgaacgct atgtccacga gctcgcccgg
     1141 cgaggcgtga tcgcccggga cgacgtggtc gatgccgcga tcgggggtga cggatcagcg
     1201 ctcaccatcc gctcggtcca gcgccatttc cggcgtacca ccggcatgac gcactcgctg
     1261 ttccgccaga tcgaacgggc ccgccgcgcg acgacgcttc tgcgcgcggg cgcacccatc
     1321 ctggacacgg tgcacgaggc cggttatttc gatcaggccc atctcagccg ttcgctcagg
     1381 gtgttcatcg gcgagacgcc ggcgagcgtc atgcgcaacg acaggcagct gtcgtttctg
     1441 tacaaaacag gctgacctgc gccgggctaa cctgccggca tgcgggtcga accatatgtc
     1501 tatttccaag gtcgctgcga ggaggcgctg gagttctatc gcgcggcact tggcgccgac
     1561 gccacggtgc tcgcccgctt cggggacaag gtgggccatg ccgaggtgcg aatcggtgac
     1621 acggttctgc tggcttccga cggtcaaggc gacggtccgt cgacgttcag cggtttctcg
     1681 ctgtcgctga gcgcatccga tgagacagag gccgaccggc tgttcgcggc gctatccgac
     1741 gggggaagcg tgcaggtgcc tattgcgccg acaccgttcg catcgcggct cggcctggtc
     1801 gccgacaagt tcggcgttcc gtggatggtc gtgtcacaga atgcggcggg gtgacctacc
     1861 catttggtag ccggcgggaa acgacatctc gatcacccgg agctggtcgt ttgctttgga
     1921 ttccagttcc aggacgacgc agtcgccgct tagttgcttg gattcgagaa cgatgtaatg
     1981 ctggccgcca ccgttgacgt cggtgacatg ttcccccgag cgcagcgcct caacgggcac
     2041 caggtcgggg gcttttaaat ggtgtggttc caccctgaaa accgtaagcg aagacaacgg
     2101 tcccggggcg gatttcgcgc gacgtgtcag ccgaccgcca tcgcatcacc cacgcctccc
     2161 aacccgaccg cgactgcgca caacacgaaa gttgcaaccg caaacggcca tccgggttta
     2221 cgccgataga gctggatgac ggtcagaacc aatccgacga ccccgatcgc gaacgcggcg
     2281 agcaatccgg tcccgacggc ggtggcggcg ccttccgcgc tgcaactctg cggcgggcag
     2341 taatccagga aggcaaggga gaacagcccc atgaccgccg cggccccgcc gaagatcacg
     2401 gtgagaatga gcgcactgat ggagatcgcg aggtcggcgc ccgcgatcgg cggctttggc
     2461 ggcggatagc tcgcgtgccc catgcgaggc gatgctatcg gccggattag cggtttctcg
     2521 ccgattccgc tgccggatcg accatcatgg cgccatcgac tgtgggggtg aagatgcaca
     2581 gcacaatgca agactttccg ctgactgtga cgggcatcct ccgtcacggc acggggtggt
     2641 actcgagtcg acgggtcacg acggcgactc ccggtggcta ccgcgagatt agttacgggg
     2701 agctcggcac acgggtagcg cagctcgctc atgggctgcg ccaactcggt gtcacggacg
     2761 gcgatcgtgt cgcgacgttc atgtggaaca accaggagca cctcgagacc tatttcgccg
     2821 cgccatgtat gggcgccgtg ttgcacacgc tgaacgtccg gctggcaggc gaccagatcg
     2881 cgttcatcgc caatcaagcc gaggatcgcg ttgtgctggt tgacatgtcg cttgcgccgc
     2941 tgctggcccc ggtgctgccg gagatgacgt cggtgcagac ggtggtggcc gtcggcgagg
     3001 gcgacgtcgc ccagctcaac acctctggga agaacgtgat ccgctacgac gacctgctgg
     3061 acggacaacc gcttgaatac agctggccag atctggacga aagaagcgcg gccgcaatgt
     3121 gttataccag cggcacgacc ggaaacccga aaggcgtggt gtacagccac cgttcgagtt
     3181 acctgcattc gatgtcggtc tgtgcggcca atgcgttggg cctcgccgac ggcgaccggg
     3241 tgttgccaat cgtgccgatg ttccacgcga acgcctgggg ccagccgtat gcggcaatga
     3301 tggcgggtgc cgacctgctg ttgcccgacc ggttcctgca ggccgccccg ctggtggaca
     3361 tgatcgaaca gcgccggccc accatcgccg ccgccgtccc gaccatctgg aacgacgtgt
     3421 tgcagtacct gcacgccaat cccggtcgcg acgtctcgtc gctgcgactg gtcgcctgcg
     3481 gggggtcggc cgtcccactc gggatgatga aggagtacga gcagtcccac agtgtgcaca
     3541 tccttcaggg ctggggcatg acggagactt caccgctggc cgcgctcgcc gctccgccgc
     3601 ccgaagtcac cggctcggac cactgggcgt tacgcagcac cgcaggccgg gttctgtgcg
     3661 gggtcgaggt gcgcctcgtc gacgacgcgg gcgccgtcct acccaatgac ggtaaggccg
     3721 tgggcgagat agagattcgc ggcccgtgga tcaccggctc ctactaccag aacgccgatc
     3781 cgtcgaagtt cgacaacgga tggcttcgaa ccggcgacgt cggccgcatc gatccgcagg
     3841 gcttcatcac gctgacggat cgtgccaagg acgtcatcaa gtccggcggc gagtggattt
     3901 cgtcggtcga gctggaactg ttgatcatgg accaccccgc ggtgctggag gccgccgtcg
     3961 tcgcggtgcc cgacgaccgg tggcaggagc gaccattggc agcggtggtt gtcaagaacg
     4021 gtgccaccgt cgcagctcct gagcttcgaa agcatctgtg cgacaaggtc gctcggttct
     4081 ggctgccgga gcgctggacg ttcatcgatg aagtgccgaa gacgagtgtc ggcaagttcg
     4141 acaagaaact catccggtcc cgatacgccg acggcgtcta cgacgtcctg gaatgccggg
     4201 actgaccagc gccactcagc gccctgcggt cagcgcccat cggataccgc cgaccacaac
     4261 gcggccggct acgcggatga cggtggcctc caccggcggg aagtagggca gcagcaacgg
     4321 cattcgcgcc cacgcgggca gcattgacac cgacgttgca gcgagcaacc cgtacggcgc
     4381 ccttgccgcg atcggcagcg gtggagtcag cagcaggaag cgggccgcct cgcgcgccgc
     4441 tgccgtccct gacaactccg gccgaaactc ctcgatccgt tcgcgcagtt ccttctgggt
     4501 cagcggtggg tccggtacgc cgagtgccct tgcgacacgg gcggtatcgg ccacataccc
     4561 gtcgcggccg ctct
//
LOCUS       DASRJA010000096         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1888531,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000096 DASRJA010000000
VERSION     DASRJA010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888531"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VFC01_04395"
     CDS             <1..972
                     /locus_tag="VFC01_04395"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HZN78920.1"
                     /translation="KLLAGDEVLLEQEYYSVGGGFIEWKGYTPPKKNPPKYPFRLMKE
                     LRDHADTNKTTIAKIMLANEMSITGRTEEEVYAFTDKIINAMVATVKSGLSMPEDDVL
                     PGPIKLHSKAATVYKRAMDDKFQADRGIGALSAFALAASEENGRGHLVITAPTGGSAG
                     VMPALVYALMEVRKLDRQKVREGMLAAAAVGYLCKHHATLSAAEGGCQAEIGVASSMA
                     AALVATAYDAPSLVVENAAESALEHHLGMTCDPVAGYVQVPCIERCAFGAVKAWTAYQ
                     IASNEIASRHRVNLDATVAALAETAKDMNSKYKETSEAGLAVSVTLC"
     gene            1164..>2098
                     /locus_tag="VFC01_04400"
     CDS             1164..>2098
                     /locus_tag="VFC01_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78921.1"
                     /translation="MTSVTLFTTSASAQSPAATTSVQTTSAAAPQPNNERYRDGIVIW
                     ETPEDAKVPFLLKFNINTQLRYLNTQSSDETFTDHLGVTREVNKRNDITVNRAMFILG
                     GYIFDQRARYSFTVWTSAGAASIVIASNIGWQFSKGLTIMAGYTGVPGSRSLVNSFPF
                     FTSTDRTMADNFFRPGFTQGAWASGEPVNGVNYLAFVGNGLNTLNISANKIDTHLLFS
                     GSVWWEPLGPYGEPGKSRNMYDDYFSSKKTRIRLGTAFTRSREDRFSNLDQSSPENTS
                     LYNSDGVLTFSTGAFAPGVTVDKATYRMWAIDGGL"
BASE COUNT          438 a          658 c          634 g          368 t
ORIGIN      
        1 aagctcctcg ccggagatga ggtgctgctg gagcaggagt actactccgt gggcggcggc
       61 ttcatcgagt ggaaagggta tacgcccccg aagaagaacc cgccgaagta cccattccgg
      121 ctgatgaaag agctgcggga ccacgccgac accaacaaga cgacgattgc gaagatcatg
      181 ctggccaacg agatgtcgat caccggcagg acggaagagg aggtctacgc cttcaccgac
      241 aagatcatca acgccatggt cgcgacggtg aagtcggggt tgtcaatgcc ggaagacgac
      301 gtgctgcccg gcccgatcaa actgcacagc aaggccgcca ccgtgtacaa gcgcgcgatg
      361 gacgacaagt ttcaggcaga ccgcggcatc ggcgcgctct ccgcatttgc gttggcggca
      421 tccgaggaaa acgggcgggg ccacctcgtg atcacggcgc cgacgggcgg ctccgccggc
      481 gtcatgccgg cgttggtcta tgcgctgatg gaggtcagga agctcgaccg gcagaaggtt
      541 cgcgagggca tgctcgcggc ggcggcggtc ggctacctct gcaagcacca cgcgacgctg
      601 tctgcggccg agggcggctg ccaggcagag atcggtgtgg cgtcgtcgat ggctgcggca
      661 ctggtcgcga cggcgtacga cgcaccgtcg ctggtggtcg agaacgccgc cgaatcggcg
      721 ttggagcatc acctgggcat gacctgcgac ccggtcgccg gttacgtgca ggtgccgtgc
      781 atcgagcgct gcgcattcgg cgccgtcaag gcctggaccg cctaccagat cgcgagcaac
      841 gagatcgcat ccaggcaccg cgtcaatctc gatgccaccg tcgcggctct ggccgagacc
      901 gccaaggaca tgaatagcaa gtacaaggaa accagcgagg ctggcctcgc cgtctccgtc
      961 acgctctgct gatcgaaggg gctcctcgct cccgcgagga gcccgtttct tccggaatcc
     1021 cggtgcttcc gctcagggag gggccggctt cagccggccc gggcgggctg aagcccgccc
     1081 ctccggctga cgggatctag acggtgggga caatacacgt gtcgatacgc cgtgccttca
     1141 ttacgacgtc agtcgcattc gccttgacgt cagtcacgct cttcacgact tctgcctccg
     1201 cacagtctcc agcagccacg acgtccgtac agacgacatc cgctgccgca ccgcagccaa
     1261 acaacgaacg ctatagagat ggcattgtca tctgggaaac tcctgaagac gccaaggtgc
     1321 cctttctgct gaagttcaac atcaacactc agctccgata tctcaacacc caaagtagcg
     1381 acgaaacctt caccgaccac ctgggcgtga cgcgcgaggt gaacaagcgc aacgacatca
     1441 cggtcaaccg cgcgatgttc atcctgggcg gctacatttt cgaccagagg gcgcgataca
     1501 gcttcaccgt ctggacatcc gccggcgccg cctcaatcgt gattgcgagc aacatcggct
     1561 ggcaattcag caagggcctc acgatcatgg caggttacac cggcgttcca ggcagtcgat
     1621 cgctggtcaa ctcgttcccg ttttttacgt caaccgacag gaccatggcg gacaacttct
     1681 tccgccccgg gttcacgcag ggagcctggg ccagcggaga acccgtgaac ggggtgaatt
     1741 atctggcgtt cgtcggcaac ggtctcaata ccctgaatat ctcggccaac aagatcgaca
     1801 cgcatctgct gttttccggc agtgtctggt gggaaccgct gggaccctac ggcgaacctg
     1861 gcaaatcgcg caacatgtac gatgattatt tctcctcaaa aaagactcgg attcgactcg
     1921 gcacggcttt tacgagatcc cgcgaggacc gtttttcaaa tctcgatcaa tcaagtcccg
     1981 agaacacgtc cttgtacaat tccgacggcg tgctgacctt ctcgacaggg gcctttgcgc
     2041 cgggtgtcac agtcgacaag gccacctaca gaatgtgggc gattgacggt gggctcaa
//
LOCUS       DASRJA010000097         5056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1613174,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000097 DASRJA010000000
VERSION     DASRJA010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613174"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VFC01_04405"
     CDS             <1..740
                     /locus_tag="VFC01_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HZN78922.1"
                     /translation="PQHTSKAAAPQIPADQVPNHVAVVMDGNGRWATQRGLHRTEGHK
                     MGEAVLIDITCGAIEIGIKHLTVYAFSTENWKRSTEEVRFLMGFNREVVRRRRENLND
                     MGVNMRWVGSRPRMWRSVIKEFDIAEQMTVGNDVITINYCVNYGGRTEIVEATRELAQ
                     EAADGKISPNRISEAMFAKHLHRSDIPDVDLFIRTSGEQRASNFLLWQAAYAEYVFQD
                     KLWPDYDRRDLWEACEEYVNRNRRFGR"
     gene            737..1150
                     /locus_tag="VFC01_04410"
     CDS             737..1150
                     /locus_tag="VFC01_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78923.1"
                     /translation="MTLGERLAAVLDDILPIQREDDGALTVHHDDTFASLRVVTIAEG
                     LDMVSLTQVVAWDLPVDTKIRNKVARHAHDTQLGTVSLVEKSTGAKRNSKKVGDVLLR
                     YNFPCAGLTDDALRTLILLVLGAGTEIRRDLVVEH"
     gene            complement(1133..1819)
                     /locus_tag="VFC01_04415"
     CDS             complement(1133..1819)
                     /locus_tag="VFC01_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78924.1"
                     /translation="MAIYEAFNSNGVSVVVSDVDLDQAAREDGPLSTELADAAAQAQR
                     SDLVEQLRRSLAGDDRARKLAAAKALLALGQYGAGELLESRAASEPDTVVAKSFTAIA
                     TRLRGPQAALDLFTNPDTDPQLARLLVSNYNSHLRLEDGDIRFLVTALRHYLARSLPW
                     LSNLRADLWDNGVFLIVNALSNDGAVHLVRATESERAELLRLLTSLPEHTDDDDIVSG
                     AKDLRSVFDD"
     gene            complement(1819..2229)
                     /locus_tag="VFC01_04420"
     CDS             complement(1819..2229)
                     /locus_tag="VFC01_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZN78925.1"
                     /translation="MGGRRPSGSGVRATRQRAAISALLEKLDDFRSAQELHDELRRRG
                     EGIGLTTVYRTLQQLAANGLVDTLRTDTGESVYRRCSDDHHHHLVCRACGSTVEIQGG
                     DVEAWATEVAREHGFSDVSHTIEIFGICGDCADK"
     gene            complement(2241..2597)
                     /locus_tag="VFC01_04425"
     CDS             complement(2241..2597)
                     /locus_tag="VFC01_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN78926.1"
                     /translation="MKTISTLSDPEVHDHGGAPLPELPSRDVLDTAGDLLRALAAPVR
                     IAIVLQLRESARCVHELVDALAVPQPLVSQHLRILKSAGVVAGERSGREVMYRLVDHH
                     LADIVVAAVTHAAEGE"
     gene            2628..4052
                     /locus_tag="VFC01_04430"
     CDS             2628..4052
                     /locus_tag="VFC01_04430"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HZN78927.1"
                     /translation="MSTLARLSLGWSPVASIIDTVANLAKRRGFVFQAGEIYGGTKSA
                     WDFGPLGVEFKENIKRQWWRSVVTGRDDIVGLDSAIILPREVWVASGHVEVFHDPLVE
                     CLNCHKRHRQDHMQEAYAAKKGIDDPESVPMSEIVCPDCGTKGEWTEPREFNMMLKTH
                     LGPIETDEGLHYLRPETAQGIFVNFTNVVTTARRKPPFGIAQTGKSFRNEITPGNFIF
                     RTREFEQMEMEFFVEPSTAPEWHQYWIDTRLQWYVDLGIDADNLRLFEHPKDKLSHYS
                     DRTVDIEYKFGFQGNPWGELEGVANRTDFDLSTHTKHSGADLSFYDQATDTRYVPYVI
                     EPAAGLTRSFMAFLIDAYAEDEAPNAKGGVDKRTVLRLDSRLAPVKAAVLPLSRHADL
                     SPKARDLAAELRKCWNVEFDDAGAIGRRYRRQDEIGTPFCVTVDFDTLEDQAVTIRER
                     DDMTQDRVAIDSVSDYLAARLKGC"
     gene            4058..4774
                     /locus_tag="VFC01_04435"
     CDS             4058..4774
                     /locus_tag="VFC01_04435"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019737516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZN78928.1"
                     /translation="MTITYDEALREQIEARLAAHERRTVTDPTKRHAAVAVVLVDSEV
                     GEDRVDPVPADGLIAELLMNIGLDGRMVDVSGGAAFLLCRRASRLNSHAAQWALPGGR
                     VDPGEAAVDAALRELEEELGVSLPESAVLGLLDDYPTRSGYVITPVVIWGGGRLDPRP
                     APDEVAAVYRVGLHQLQREDSPRFITIPESSRPVVQIPLGHDLIHAPTGAVLLQLRWL
                     CLEGRNDPVDGLEQPVFAWK"
     gene            complement(4777..>5056)
                     /locus_tag="VFC01_04440"
     CDS             complement(4777..>5056)
                     /locus_tag="VFC01_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZN78929.1"
                     /translation="IAHANGASMLAAQAQDLANADVRAAQRSYTGQYGSTSGSNMGAV
                     LGGIIIGNILSGALRGGIGGGMGGGWSSTSFGGSGSSGGWSGGGGGRF"
BASE COUNT          904 a         1611 c         1691 g          850 t
ORIGIN      
        1 caccgcagca cacctcgaag gccgccgcgc cgcagattcc ggccgatcag gtacccaacc
       61 acgtcgccgt cgtcatggac ggcaacgggc ggtgggccac ccaacgcggg ctgcaccgca
      121 ccgagggcca caagatgggc gaagcggtgc tcatcgacat cacctgcggg gccatcgaaa
      181 tcggcatcaa gcacctgacg gtctacgcgt tctccaccga gaactggaag cgcagcaccg
      241 aagaggtccg cttcctgatg gggttcaacc gtgaggtggt ccgcaggcgg cgcgaaaacc
      301 tcaacgacat gggcgtgaac atgcgttggg tgggatcgcg gccgcgcatg tggcgcagcg
      361 tgatcaagga gttcgacatc gccgagcaga tgacggtcgg caacgacgtc atcacgatca
      421 actactgcgt gaactacggg ggccgcaccg agatcgtcga ggcgacacgc gaactcgcgc
      481 aggaagctgc ggacggcaag atcagtccga accggatcag cgaggcgatg ttcgccaagc
      541 acttgcaccg ctccgacatt cccgacgtcg acctgttcat ccgcacgtcg ggggagcagc
      601 gggcgagcaa ctttctgctg tggcaggcgg catatgccga atatgtcttt caggacaagc
      661 tgtggccgga ctacgaccgc cgcgatctgt gggaagcgtg cgaggagtac gtgaaccgga
      721 accgacggtt cggcagatga cgctcggcga gcgactggcc gcggtcctcg acgacatcct
      781 gccgattcaa cgcgaagacg atggtgcact caccgtgcac cacgacgaca cgttcgcttc
      841 actgcgcgtg gtgacgatcg ccgagggtct cgacatggtg tcgctgacgc aggtggtcgc
      901 gtgggatctg ccggtggaca ccaagattcg taacaaagtt gccaggcatg cccacgacac
      961 ccagctgggc acggtgtcgc tggtggagaa gagcacgggc gccaagcgca attccaagaa
     1021 ggtgggcgat gtgctgctgc gctacaactt tccctgcgca ggcctcaccg acgatgcgct
     1081 gcgcacgttg atcctgttgg tgctgggcgc cggtaccgag attcgtcgcg acctagtcgt
     1141 cgaacactga ccgcagatcc tttgcaccgc tgacgatgtc gtcatcgtcg gtgtgctcgg
     1201 gcagcgatgt aagcaggcgc agcaactccg cccgctctga ctcggtagcg cgcaccagat
     1261 gcacggcgcc gtcgttcgac agcgcgttga cgatcaggaa gacgccgttg tcccagaggt
     1321 cggcgcgcag gtttgacagc caaggcaggc tgcgcgcgag gtagtgccgc agcgcggtga
     1381 cgaggaaacg gatgtcgccg tcctccagtc ggaggtggct gttgtagttg gacaccagca
     1441 ggcgggctag ctgcgggtcg gtatcgggat tggtaaacag gtcgagcgcc gcttgtggtc
     1501 cgcgcagccg cgttgcgata gcggtgaagc tcttggcgac gacggtgtcc ggctctgacg
     1561 cagcgcggga ttcgagcagt tcaccggcgc cgtactggcc taaagccaga agtgccttgg
     1621 cggcggccag ttttcgcgcg cggtcgtcac cggcgaggct gcgtcgcaat tgctcgacga
     1681 gatcagaccg ttgagcctgc gccgccgcat cggcaagctc cgtgctgagc ggtccgtcct
     1741 cgcgggcggc ctgatcgagg tcgacgtcgc tgaccaccac gctgacgccg ttgctgttga
     1801 acgcctcgta gatcgccact acttgtcggc gcagtccccg cagataccga agatctcgat
     1861 ggtgtggctg acgtcggaga agccgtgctc acgggcgact tcggtggccc acgcctcgac
     1921 gtcaccgccc tggatctcga ccgtcgagcc gcaggcgcgg cagaccagat ggtgatggtg
     1981 atcgtcggag cagcgccggt acaccgactc gccggtgtcg gtgcgcaggg tgtcgacgag
     2041 cccgttggcg gccaactgtt gcagcgtgcg gtacacggtc gtcagcccga tgccctcgcc
     2101 gcggcggcgc agttcgtcat gtagttcttg tgcggaccgg aagtcgtcga gcttctccag
     2161 cagcgcggag atcgcggcgc gctgccgggt ggcccgcacc ccggagccgc ttggccggcg
     2221 acctcccatc gcggtctggc tcactcgccc tcggcggcgt gcgtgaccgc tgcgacgacg
     2281 atgtcggcga ggtggtggtc caccagccgg tacatcacct cgcgtcccga acgctcgccc
     2341 gccaccacgc cggccgactt gaggatccgc aggtgctggc tgaccagggg ctgcggcact
     2401 gccagtgcgt cgacgagctc gtgcacacag cgcgccgact cacgcagctg cagcacgatc
     2461 gcgatccgta ccggcgccgc caaggcacgc agcaggtcgc ccgcggtgtc gagaacgtcg
     2521 cgcgacggaa gctccggcag tggagcgccg ccgtggtcgt gcacctcagg atcggacaaa
     2581 gtggaaatcg ttttcattat gcggccagaa tacatgcgcg accgcgcatg tcaacgttgg
     2641 cgagactgtc gctaggctgg tcacccgtgg cgtccatcat cgacaccgtt gcgaacctgg
     2701 ccaaacgtcg tggctttgtg tttcaggccg gcgaaatcta cggcggcacg aaatcggcgt
     2761 gggacttcgg gccgttggga gtcgagttca aggagaacat caagcggcag tggtggcggt
     2821 cggtggtcac cggccgcgat gacatcgtcg gtctcgacag cgcgatcatc ttgccgcgcg
     2881 aagtgtgggt ggcctcgggt cacgtcgagg tgttccacga tccgctggtc gaatgcctga
     2941 actgtcacaa gcgccaccgg caggaccaca tgcaggaggc atacgccgcg aagaagggaa
     3001 tcgacgaccc ggaatcggtg ccgatgtccg agatcgtctg cccggactgc ggcaccaagg
     3061 gtgaatggac cgagccgcgc gagttcaaca tgatgctcaa gacgcacctc ggcccgatcg
     3121 agaccgacga gggcctgcac tacctgcggc ccgaaaccgc gcagggcatc ttcgtcaact
     3181 tcaccaatgt ggtcaccacg gcgcgccgca agccgccgtt cggtattgcg cagaccggca
     3241 agagttttcg caacgagatc accccgggca acttcatttt ccgcacgcgt gagttcgagc
     3301 agatggagat ggagttcttc gtcgagccgt cgaccgcacc ggagtggcac cagtattgga
     3361 ttgacacgcg gctgcagtgg tacgtcgacc tcggcatcga tgccgataat ctgcggctgt
     3421 tcgagcatcc gaaggacaag ctctcgcatt actccgatcg cacggtcgac atcgagtata
     3481 agttcgggtt ccagggcaac ccgtggggtg agctcgaagg cgtggccaac cgcactgatt
     3541 tcgacttgtc aacgcacaca aagcattccg gtgccgacct gtcgttttac gaccaggcca
     3601 ccgacacccg gtacgtcccg tatgtgatcg agcctgcagc cgggctgacg cggtcgttca
     3661 tggcgttcct gatcgacgca tacgccgagg acgaggcgcc gaacgccaag ggcggcgtgg
     3721 acaagcgcac ggtgctgcgg ctggattcgc ggctggcgcc ggtcaaggcg gcggtgttgc
     3781 cgctgtcgcg gcacgccgat ctgtcgccga aggcgcgaga tcttgccgcc gagttacgca
     3841 agtgctggaa cgtcgaattc gacgacgccg gcgcgatcgg caggcgctac cgccgccagg
     3901 acgagatcgg cacgccgttc tgtgtgacgg tcgacttcga cacgctcgag gatcaggcag
     3961 tcacgatccg cgagcgcgac gacatgacgc aggaccgcgt cgcgatcgac agtgtgagcg
     4021 actatctggc ggcgcgactc aagggctgtt gaccgaggtg acgatcacct acgacgaggc
     4081 tctgcgggaa caaatcgagg cacgcctggc ggctcacgaa cgtcgcacgg tgaccgaccc
     4141 gacgaagcgg cacgcggccg tcgcggtggt cttggtcgac tccgaagtcg gtgaggacag
     4201 ggtcgacccg gtgcctgccg acgggttgat cgctgagctc ctgatgaata ttggtctcga
     4261 cggccggatg gtcgatgtct ccggcggcgc cgcctttctc ctgtgccgca gggcgtctcg
     4321 gctcaactca catgccgcac agtgggcgct gcccggcggc cgcgtggacc ccggtgaagc
     4381 cgcagtcgat gccgcactgc gggaactcga agaagagctc ggcgtctcgc tgccggagtc
     4441 ggccgtgctc ggcctgctcg acgactaccc gacgcgttcg ggatatgtga ttacaccggt
     4501 ggtcatctgg ggcggaggtc gtctcgaccc tcgccctgca cccgacgagg tggcggcggt
     4561 ctatcgggtc ggactacacc aactgcaacg cgaggattcg ccccggttca tcacgattcc
     4621 cgagagttcg cgtccggtgg tgcagattcc cttgggacac gacctcattc atgcgccgac
     4681 gggtgctgtc ctgctgcaac tccggtggct gtgccttgaa ggtcgcaacg atccggtcga
     4741 cggacttgag caaccggttt tcgcctggaa gtagtactag aaccgcccgc cgccgccacc
     4801 gctccagccg cccgacgacc cggagccgcc gaacgacgtc gaactccagc cgccgcccat
     4861 gccgccgccg atcccgccgc gcagcgcccc ggacaggatg ttgccgatga tgatgccgcc
     4921 gagcaccgcg cccatgttgg atccgctggt gctgccgtac tgaccggtgt aggaccgctg
     4981 cgctgcacga acatccgcgt tggccaagtc ttgcgcctgc gccgccagca tggaggcgcc
     5041 gttggcgtgc gcaatc
//
LOCUS       DASRJA010000098        18838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3582669,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000098 DASRJA010000000
VERSION     DASRJA010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18838
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3582669"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="VFC01_04445"
     CDS             complement(<1..1254)
                     /locus_tag="VFC01_04445"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HZN78930.1"
                     /translation="MSAQTNTGTLGTTGTTEPTHTEQPTTAPTETAPPTPEPTQTPET
                     EEPTQPQGDPTPTPTNSNKASSNSDAGPSTSPSAHIGQPAADPSLALQSPDPVAVFAD
                     ARTLAAASPTMMSPMMATQGLSVEPFQPAVAAPAPNPLTAITNLVSSVINFVLNPFAG
                     GAPTTPAQPPLIWSLLAFARREFENFFNGLSGRSAANVLAAPQSTSLTLDNAEMTSFA
                     AAAVAPDTLYLPFATAQVSPSTRFVSWVTGGYVYDDLTLANTLSRFSIYGTDVGTMWD
                     NGIPDNPATVGINEHQVLIAVGDTFVGPNSADWRANTILRSSDTDLFDGITIPNGQWY
                     TGNWFGGAPLGPDGFPNRARQVIFASGLPAGVTLIPTAGISVPTPGTEFGVTQYMSFM
                     SVTQWGAPGQWTTNYSALAYSTDNGE"
     gene            1268..1528
                     /locus_tag="VFC01_04450"
     CDS             1268..1528
                     /locus_tag="VFC01_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78931.1"
                     /translation="MTVFVVLPGGVVSGSGSVVTGVVDGSVEVCVLVVVVVVLVVVPV
                     DPLVVSDGGGFDAVSSADATPYPVAMAVPTPNATASPPTRPI"
     gene            complement(1717..2082)
                     /locus_tag="VFC01_04455"
     CDS             complement(1717..2082)
                     /locus_tag="VFC01_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78932.1"
                     /translation="MAEAGFWIAWGLPTQGREKQALDLLHESRGYLAGLAQDRQIERF
                     DIAILKPQTTELGGFILIQGTQSQIDSLRRSDDFQVWVNRVQLVADRVGMVDAWVGEG
                     LSEATGLYEQALRKAGLSP"
     gene            2203..2913
                     /locus_tag="VFC01_04460"
     CDS             2203..2913
                     /locus_tag="VFC01_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN78933.1"
                     /translation="MTGPRIARFFGAVVVTSWATLLCAPLPFASAQPCPDVEVVFARG
                     TSEPPGVGGVGQAFVDALRSQIGTRSMDVYPVNYAASSDFGDRMGIARTVVDGIRDAG
                     AHVEATAANCPNTRIVVGGYSQGAVVAGFVTSATIPDAVPAEFRSSVPNPMPPSVADH
                     VAAVVLLGRPSDQFMRDGGAPPVVIGPLYVPKTIDLCIPDDTICNGAPAGGPSISHAL
                     YGMNGMTNQAAEFAAGRL"
     gene            complement(2917..4500)
                     /locus_tag="VFC01_04465"
     CDS             complement(2917..4500)
                     /locus_tag="VFC01_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HZN78934.1"
                     /translation="MTQLEAPTQTGRKLSQLDDAERYRCFDELQLTMPDVWESMRRDM
                     EDESVVVVPSISIERTTPGSGTIMQAMEERALFLLLLLRQPRLRMIYVTSQPVSEAII
                     EYYLGLLPGVIPSHARARLTLVPVGDASGVSLSSKLLARPRLLREIRSLIPNASRSHL
                     IPYNTTPLERDVALSLKIPMYGADPRLEDLGSKTGCRRMFEELGVRCPVGAEDLHSVD
                     EIVAGVQSMRARRPSLHQAIVKLNEGVSGAGNAMVYLSDLPAPDSPDEAAVIRDRVLS
                     LQPESEKLSVAEYLAAFEKHGGIVEERITGEKLESPSVQMRALPDGTVELLSTHDQLL
                     GGASGQKYLGCVFPADPDYAAAIAEPAMVVGRRLAELGALGRFAVDFVVVQDNTGAWT
                     PYAIELNLRKGGTTHPFLTLQFLTDGTYDGERGVFLTPSGSTKYLIATDHFEDDRLKS
                     LTVRELFDVVVRHGLHFDQARRTGVVFHMISCLTECGRVGLTAVGDTPEDATRLYEKA
                     QSVVLQEAEQALEEGSVVG"
     gene            complement(4509..5618)
                     /locus_tag="VFC01_04470"
     CDS             complement(4509..5618)
                     /locus_tag="VFC01_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN78935.1"
                     /translation="MDIKAIVLAVDDQPTNLRLLDAVLTPRGHRVVTAASGAEALALL
                     ETEDIDIVLLDILMPEMDGYEVCRRIRSTPATEFLPVVMITASGAEQRLAALEAGADD
                     FVTKPFDKSELLARVASLARIKQYHDTIRRQADELAAWNTELESRVTQQVAELERTNR
                     LRHFLSPQLADLVVGEENMLNSHRREIVVLFTDLRGFTSFAETSEPEEVMGVLGEYHH
                     AIGALIHAYGGTLERFTGDGIMVFFNDPIECDDAAERAVRTALEIRDAVTDLAARWRR
                     KGYDLPVGIGIAQGYATLGRIGFEGRFDYAAIGSVTNLAARLCSDAGPWQVLVTDRVL
                     AATEHLAIAEEVGDIQPKGFSRTVRVHNISGLKEE"
     gene            complement(5618..5998)
                     /locus_tag="VFC01_04475"
     CDS             complement(5618..5998)
                     /locus_tag="VFC01_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN78936.1"
                     /translation="MTPARILVVEDNLLNLKLVRDVLTFASYDVIEAQSGEEALRAAK
                     ETPPDLVLMDLQLPGIDGTETLHRLRESTLGSDVPVVAVTALAMAEDKERAARAGFDG
                     YIEKPISARALPGQIEAFLNGRAT"
     gene            complement(5995..8193)
                     /locus_tag="VFC01_04480"
     CDS             complement(5995..8193)
                     /locus_tag="VFC01_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZN78937.1"
                     /translation="MGERSERTTRVSRASTELNQQLRDAREQSAASREILAALGRADA
                     DPEAILDTILERAAHLCRAQAATLYIPEGDVFRLSRDTGKIPEEFRKLLLDQPLARNR
                     SSTVGRAAEEMRTIQIPDVFKDAEYLRLDLQSLAGFRTLLSTPMMLQNEVVGVLSMWR
                     TNVKPFNARECELLEEFAVQGAIVMRQDALRRALKSKLKQLEALREVGDAVGSTLDLD
                     EVLDQIVSNAVRLTGTDGGSIMEYDESSDSFHVRAAAGSSPDLLKRLRAITIDRETTL
                     VGRAATDHQTVEVPDLAAKTELDPHLDALFRDGWRSVLAVPILRGERILGALVIRRRS
                     TGSFGPHVELLETFAGQSALAIVNARLFGELQTKTSELEIASQHKSEFLASMSHELRT
                     PLNAVIGFSEVLLDRMFGEINERQEDYLRDIWNSGKHLLELVNDILDLSKVEAGQMVL
                     EPSTFSVSSALDYCRAMLRERATSHAITIGVEVADGIDTIESDELLFKRVVLNLLSNA
                     VKFTPDKGTVTVRAYGEGTDLLVTVTDTGIGVPAEDQERIFESFQQGRRGAPKEEGTG
                     LGLTLSRRIVWLFGGRMWLESTPGKGSTFGFSVPGMLRPAPEPHVSRHLPVVLLVDDD
                     RASLDLNSAYLDGSPIRVTRAMDGVEALELARKELPAAVVLEIGLPRLDGWQVLTELK
                     ADPATATIPVVIATDVDDRARGLALGAKVYLRKPIRREELVDALQQVGVL"
     gene            8332..8667
                     /locus_tag="VFC01_04485"
     CDS             8332..8667
                     /locus_tag="VFC01_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZN78938.1"
                     /translation="MNSTCDVRGMHTTLMGYASAAGEQPTEVYRVGLRAVPFLLSVAD
                     LLIGWLLLWHAEVALDAGASERDGAFCRGKIAAAVLFAETVLPRLAADRIVVESQSLA
                     AMELDEEAF"
     gene            complement(8670..9623)
                     /locus_tag="VFC01_04490"
     CDS             complement(8670..9623)
                     /locus_tag="VFC01_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN78939.1"
                     /translation="MDTHRLKYFLRIAEEGSITRAAAALGVAQPALSRQIRLLEEDLG
                     VSLFRRTRRGVQLTDEGERLRASTAAPLRQLELAVQYAGSPVAKIERGVRLGLPETTV
                     DVLAAPLITSLSTVFSNLTFSVTAGTTAALVDAMLKGAVDVALINPVPDDRVFYRDLV
                     AEDLVVVGGPACDLDTGRALTFIELVALPLVIPRSHAGIGTLLENAALRVKVNIGYRV
                     TTDSLQVTKRLIEAGLVYGVLPLSACGREIERGQMRYAPLREPTLTQHLGVAATSQLE
                     LPREFTVKLGNTLREEAARLIKAGHWQAQLLSPQAWDPHRA"
     gene            9784..10659
                     /locus_tag="VFC01_04495"
     CDS             9784..10659
                     /locus_tag="VFC01_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN78940.1"
                     /translation="MTVQEDQDWAQVLGFDRLPEHIVGKRVTELMDLSGKRAFVTGAG
                     GDGLGQAIANRLAGLGADVALIGRTLEKVERRAKEIEQRWGVNAYPVRADMSDWDQVH
                     DAVREAHGELGGLDIMVNNPVMVKAGPFEKQTKEDIDYTVLGSLTMMMYGAHAALEFL
                     LPQGSGKIINIGSVGGRIQQRGLVVYNACKSGVIGFTRNLAHEVALRGVNVLGVAPGI
                     MLKPDMKRYMLNPQNDQDRAGRGAVAEAITQQVQLGRASLPEEAANVVAFLATEAADY
                     LCGQTIDVAGGQWMN"
     gene            10663..11799
                     /locus_tag="VFC01_04500"
     CDS             10663..11799
                     /locus_tag="VFC01_04500"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZN78941.1"
                     /translation="MLSVNEVEDAAASKTGLSDFGEPSYREGLGLLLKSLGAEARLNG
                     RGEAFIYQRIIGYLGQRLQVENWFRRHPEIDELPIAAPLIGLGLPRTGSTALSMLLAQ
                     DPGVRYLRKWESSQPCPPPSTVQGVDPRIPPDKGEMIGTRYHVPTDTHGPMECHELMA
                     LDFKSHLFQSFAQVPSYSEWLIEKADLESTLSYQKRVMKLLQWGEPAKPWRLKCPSHV
                     LWLGAVHSAFPDAKYVMTHRDPTDVILSVADLYADIIGSFTDDIDRRYIGRLNVEHWS
                     LGMDRALRFRANGADDRFYDIDFRAMQADPIGEVKGLYAWLGEPVGDEFESRMQSWWT
                     RAAAEREPSSHADPVEFGIELDAVRPRFATYVDHAVRWTAHQSA"
     gene            11887..12876
                     /locus_tag="VFC01_04505"
     CDS             11887..12876
                     /locus_tag="VFC01_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78942.1"
                     /translation="MRDSVSFWTVDDRGEIGLPRIGIEAVAANWDNHDIQVNVAFPDG
                     RVYRLRGNGPTLPAEGPGGQPTELGAGGLVFRCLKPFDTWTMSYDGKAVQTSSADLVA
                     GRTDGPLVDVAFEVKATMAVPPWVQGALQADAGTQLKTSVEGDLMGGPRYEQLFRAVG
                     SVTVDNGDAQQFTGSGLRIRRQGVRRLAGFWGHCWQSAVFPSGKAFGYIAYPPRPDGQ
                     PTFNEGYLFTGDGALIPATVVQAPWLTRLQALGEDVSLVLETNSGTVQIEGETVFSTH
                     DIHHNDDTYSVHAMKNEIPDFPALQQAGVRYRWDGEETYGMLERSNPLDKISR"
     gene            12873..13643
                     /locus_tag="VFC01_04510"
     CDS             12873..13643
                     /locus_tag="VFC01_04510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN78943.1"
                     /translation="MNGLDGKSFVIAGGATGIGAGTAKRLAEEGASVVVGDINIGGAK
                     ATVDDIVRSGGRAVAVEFDLADEASVQRLVDAAIAEFGAVHGLHNVGADLSENNLGRD
                     KTLLDTGFDVWHRTLDVNLLGYVRTIRAVLPHLLERGGGNIVNTSSGGSLGSDPSHVA
                     YCASKAAVNHLTRHVANNWGKQGIRCNGVMPGLVMGETQERHNDQQIQQMFLMAAKVT
                     RLGKPSDLAAITAFLLSDDAEWINGQVWYIGGASHMRQ"
     gene            complement(14101..15186)
                     /locus_tag="VFC01_04515"
     CDS             complement(14101..15186)
                     /locus_tag="VFC01_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN78944.1"
                     /translation="MYQGQPTWVVSRYHDIRAALVDPRLSAETIPASIMPATADGETP
                     VMFARIDDPEHHRLRRMMTRDFTFRRADSMRPQIQGIVDRYLDEMIDNGPPSDLVRDF
                     ALPVPSLVIALLLGVPSDDLEYFHRNTTVSLDTRSSDEQKAQAFGAMCTYIRELVDLK
                     EHNPGDDLMSRLATEYVATGQLTRETAAVTGAIMMQAGHETTANMIALGAVALVQHRD
                     VFERLGQTDDQAVIANAVEELMRYLTIVHSQVDRLATEDLTIGGQLVHAGDLLVMNLP
                     AGNWDPDFVDDPETLDIDRNTRGHVGFGYGVHQCIGQNLARVEMQIAFATLARRLPGL
                     RLNAALDMLQFKGESGIYGMKELPVTW"
     gene            15412..16038
                     /locus_tag="VFC01_04520"
     CDS             15412..16038
                     /locus_tag="VFC01_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN78945.1"
                     /translation="MTAVADRPLRADAARNAERILRAAREAYGELGPEAPVEAIARRA
                     GVGERTLYRRFPTKADLISAALEQSIAEDLTPTIEDARRATDPLQGLAQLIEAAISLG
                     AREHHLLTAARRAGSLTMDISTPMYDVLTELADRAQEAGLVRADLVADDLPRLIAMLY
                     SVLSTMDSTGDGWRRYVALILDAITVNDSRPLPPAVALRYQPLTSTWP"
     gene            complement(16043..16387)
                     /locus_tag="VFC01_04525"
     CDS             complement(16043..16387)
                     /locus_tag="VFC01_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78946.1"
                     /translation="MNRISRLTTTVLVSGGLGLASLGLGAGIAHAGGPYQWCPGDEAG
                     GYGGGFNTPADARPNWDWNVCHTYHYVNPGQGNVSSTVWDGDNPPPPYPWQAPNMCWS
                     LFIPRPCGPNGY"
     gene            complement(16526..16654)
                     /locus_tag="VFC01_04530"
     CDS             complement(16526..16654)
                     /locus_tag="VFC01_04530"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN78947.1"
                     /translation="MADQEGQRVSLGPGSLRVLPRGWRDSWVVRETIRKSYITVDH"
     gene            complement(16659..18074)
                     /locus_tag="VFC01_04535"
     CDS             complement(16659..18074)
                     /locus_tag="VFC01_04535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN78948.1"
                     /translation="MSIDENPVDAPTEFVNGDVSFWYRSIGLPDRRPPLPGDRDADVC
                     IVGAGFTGLWTAYYLKQAQPDLRIVLLDREFAGFGASGRNGGWLSAELAGKRDAYAAT
                     HGHQGVVDLMRAMRGAVDEVIAVAKGEGIDADIAKDGLLHVARNRAQLGRLHESLRYE
                     QDWGAGPEDFVALSRDEMDSRIRVAGALGGLFSPHCARVQPAKLVQGLARVVEATGVT
                     IFEQTAVNRIEQGRAVTDLGTVRAPIVLRCLEGFTASIEGQRRNWLPMNSSMVVTAPL
                     PKDAYDQIGWQDAELLGDYAHGYMYAQRTADNRIALGGRGIPYRYGSRIDDRGATQHW
                     TVEALTALMHDMFPATTDVPIVHAWCGVLGVPRDWTATVDLDRSTGLGTAGGYVGSGL
                     TTTNLAGRTLADLVLERDTELTRLSWVGRHVRRWEPEPLRWLGVQSMYVLYRMADRRE
                     TEKNLAHTSRIARIANMITGR"
     gene            complement(18214..18792)
                     /locus_tag="VFC01_04540"
     CDS             complement(18214..18792)
                     /locus_tag="VFC01_04540"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN78949.1"
                     /translation="MESPEDSVPLTAESAMGARIREFRQARKLTLRELAVRAATSPGF
                     LSQLERGQVNASVGTLRRLCEGLGITLPDLFTDHPASGPRILRKHDRPEIHVSDTSAK
                     YLLSQKPLRNLEVYVAEFAPGGTAGEAYVHDDSQEILLVISGSVVLELDGAKHRLDAG
                     DSAEYRTSIPHTVHNTGDERAELLWIVSPPTS"
BASE COUNT         3228 a         5930 c         6245 g         3435 t
ORIGIN      
        1 ctcgccgttg tccgtcgagt atgcgagcgc cgagtagttc gtcgtccact ggccgggagc
       61 accccactgg gttaccgaca tgaagctcat gtattgcgtc acgccgaact cggtccccgg
      121 cgtcgggacg gagatgcccg cagtcgggat gagggttacc cctgcgggca gaccgctggc
      181 aaagatgact tgtcgcgctc ggttcgggaa accgtccggg cccaacggcg ctccgccgaa
      241 ccagttgccc gtgtaccact gcccgttggg aatggtgatc ccgtcgaaca ggtcggtgtc
      301 cgagcttcgc aagatcgtgt tcgcgcgcca atccgccgaa ttgggcccga cgaacgtgtc
      361 gccgaccgcg atcaatacct ggtgctcgtt gatgcctacc gtggcgggat tgtcgggaat
      421 gccgttgtcc cacatggtcc caacgtcggt gccgtagatc gagaagcggc tcagagtgtt
      481 ggccagcgtc aagtcgtcgt aaacgtatcc acccgtcacc cagctcacga accgcgtcga
      541 cgggctcacc tgagcggtgg cgaatggcag atacagcgtg tcgggagcca ccgcagccgc
      601 cgcgaagctg gtcatctccg cgttgtcgag cgtgaggctg gtgctttgcg gcgccgcaag
      661 gacgttcgcc gcggagcggc cgctcaagcc gttgaagaag ttctcgaact cacgccgtgc
      721 gaaggcgagc aggctccaga tcagcggcgg ttgggccggt gtcgtcggcg cgccgccggc
      781 gaacgggttc agcacgaagt tgatgacgct cgagaccagg ttggtgatgg cggtcagcgg
      841 attcggggca ggggcggcga cggccggttg gaatggctcg actgacaagc cttgggtcgc
      901 catcatcggc gacatcatcg tcggcgaggc ggcggcgagc gttcgtgcgt cggcgaagac
      961 ggcgacggga tctggcgact gaagggccag agagggatct gcggccggtt ggccaatgtg
     1021 cgcggacggc gaagtgcttg gcccggcgtc gctgttcgac gacgctttgt tgctgttggt
     1081 cggcgtcggc gtcggatcgc cttgtggttg tgtcggctct tcggtctcgg gagtctgcgt
     1141 cggctccggt gtgggtggcg ccgtttctgt gggcgctgtc gtcggctgtt cggtgtgtgt
     1201 cggttccgtc gtcccggtgg tccccaatgt gccggtgttg gtctgggcgc tcactacggt
     1261 gccgccggtg acggtgttcg tagtgctgcc cggcggcgtg gtgtccgggt ccgggtctgt
     1321 ggtgaccggt gtggtcgacg gttccgtcga ggtctgtgtg ttggtggttg tcgtggtggt
     1381 gttggttgtg gtgccggtcg acccgctggt cgtatcagac ggcggcgggt tcgacgcggt
     1441 gtcgtcggcc gatgcgacgc catacccggt ggcgatggcc gtcccgacgc ccaacgcgac
     1501 tgcgagcccc ccgacgcgac cgatataggt tgcggctccc atgttgtccc tccaaaaagc
     1561 aactcccccg tcaatttgcg atgtgaggtc attaaatcga atggagacga agcgcacagc
     1621 aaaagggaca aatgactcat gaatcacagg cgtcgctgtg gttttgatct tgttgggctt
     1681 ttggaagggc cgatgtcggt tgagctaccc cgctcgctac ggggagaggc cggcctttcg
     1741 gagcgcctgc tcgtacaggc cggtcgcctc gctgaggccc tcgccgaccc aggcgtccac
     1801 catcccgacc cggtcggcaa ccagctgcac ccggttgacc cagacctgaa agtcgtcgct
     1861 acggcgtaac gagtcgatct gcgactgcgt gccctgaatc aggatgaagc cgcccaactc
     1921 tgtggtctgt ggcttgagga tggcgatgtc gaaacgctcg atctgccggt cttgcgcgag
     1981 accagcgagg tagccccggc tctcgtggag caggtcaagt gcttgttttt cgcgtccctg
     2041 cgttggtaga ccccacgcga tccagaaccc ggcttcggcc atggcctcta cgtttatcag
     2101 acgccgtggc cgtcctacga gcgttttgcc cggcaaacat ccgggtacat ggtcaaccgg
     2161 cggcggcagt caaccgccat ccgcgggtca aggagttcgc taatgaccgg tccccgaatc
     2221 gctcgttttt ttggtgcagt ggtcgtgaca tcctgggcga cgctgttgtg cgcacctctc
     2281 ccattcgcat ccgctcagcc atgccccgac gtcgaggtgg tgttcgcccg cggcaccagt
     2341 gaaccacccg gggtcggtgg ggttggccag gcgttcgtcg acgcgttgcg ctcccagatc
     2401 ggcaccagat cgatggacgt gtatccggtc aactacgccg ccagcagcga tttcggcgat
     2461 cgcatgggga tcgcgagaac ggtcgtcgac gggataaggg acgccggcgc tcatgtggag
     2521 gcgacggcgg cgaactgccc caacaccaga atcgtcgtcg gcggatattc ccagggtgcc
     2581 gtcgtcgcgg gtttcgtgac ctcggcgacc attccggacg ccgtcccggc cgagttccgg
     2641 tcctccgtac cgaatccgat gccgccttcg gtggcagacc acgtcgcggc cgtcgtcttg
     2701 ctcggcaggc cctccgatca gttcatgcgc gacggcggcg cgccgccggt agtgatcggc
     2761 ccgctatacg tgccgaagac aatcgacctg tgcattcccg acgacacgat ctgcaacggc
     2821 gcgccggcgg gtggccccag catctcgcac gccctgtacg gcatgaacgg catgaccaac
     2881 caggccgcgg agttcgcggc aggtcggctc taggtctcag ccgacgacgc tgccttcctc
     2941 gagcgcctgt tcggcctcct gcaggacgac ggactgcgcc ttctcgtaca gccgcgtggc
     3001 gtcctcgggg gtgtccccga cggcggtcag gccgacccga ccgcactcgg tcaggcagct
     3061 gatcatgtgg aacaccacgc ccgtgcgccg ggcctgatcg aaatgcaggc cgtgccgcac
     3121 gacgacatcg aacaattccc gcacggtcag tgacttgagc cggtcgtcct cgaagtgatc
     3181 ggtggcgatc aagtacttgg tgctgccgct gggggtcagg aacacgccgc gctcgccgtc
     3241 gtaggtgcca tcggtgagga attgcagggt gaggaatgga tgggtggtcc cacccttgcg
     3301 gaggttcaac tcgatcgcat acggcgtcca cgcgccggtg ttgtcctgca ccacaacgaa
     3361 atccacagcg aacctgccga gcgcaccgag ctctgcgagc cgtctgccga ccaccatggc
     3421 gggttccgcg attgccgctg cgtagtccgg atcggccggg aacacgcagc cgaggtactt
     3481 ctggccgctc gcgccgccga gaagctggtc gtgggttgac agcagttcga cggtgccgtc
     3541 gggcaacgcc cgcatctgga cgctcggact ttccagtttc tccccggtga tccgctcttc
     3601 gacgatgccg ccgtgttttt cgaacgcggc cagatattcc gctaccgaga gcttttccga
     3661 ctccggttgc aagctcagca cgcggtcccg aatgacggcc gcctcgtcgg gcgagtcagg
     3721 ggccggcagg tcgctcagat acaccatcgc gttgcctgcc ccggagacgc cttcgttgag
     3781 tttcacgatt gcctggtgca gcgacggccg ccgcgcgcgc atgctttgca cgcccgcgac
     3841 gatctcatcc accgagtgaa ggtcctcggc cccgactgga cagcgcaccc cgagttcctc
     3901 gaacatcctg cggcagccgg tcttgctgcc gaggtcctcg agccggggat ccgcgccgta
     3961 catcgggatc ttcaggctca gggcgacatc gcgctcgagc ggtgtggtgt tgtacgggat
     4021 gaggtggctg cgcgatgcgt tgggaatcag cgaccggatt tcgcggagaa gtcggggccg
     4081 cgcaaggagt ttcgagctga gcgaaacgcc tgacgcatcg ccgaccggca ccagggtgag
     4141 ccgggcgcgg gcatggctgg gaatcacgcc gggaagcaac ccgaggtagt actcgatgat
     4201 cgcttcggac accggctgcg aggtcacgta gatcatccgc agccgcggct ggcggagcag
     4261 cagcaagagg aacagcgcgc gttcctccat cgcctgcatg atggtgccgc tgccgggtgt
     4321 ggtgcgttcg atgctgatcg acgggaccac gacgaccgat tcgtcctcca tgtcgcgcct
     4381 catggactcc cagacatccg gcattgtcag ctgcagttca tcaaagcagc gatagcgctc
     4441 cgcatcgtcg agctgcgaca gtttgcggcc cgtctgggtg ggtgcctcta gttgtgtcat
     4501 ctgcctacct actcttcttt gagcccgctg atgttgtgga cccgcaccgt gcggctgaat
     4561 cccttgggct gtatgtcgcc gacttcttct gcgatggcta ggtgctcggt ggcagcgagt
     4621 acgcggtcgg tgaccaacac ctgccacgga ccggcgtccg aacacagccg ggctgccaga
     4681 ttggtgacgc tgccgatggc ggcatagtcg aatcggccct cgaagccgat ccgcccgagc
     4741 gtcgcataac cttgggcgat accgattccc acgggcaggt cgtagccctt acgacgccag
     4801 cgagcggcaa ggtcggtgac ggcgtcccgg atctccagcg ccgttcgcac ggcacgctcc
     4861 gcggcatcgt cgcattcgat tgggtcgttg aagaacacca tgatcccgtc gcccgtgaac
     4921 cgttcgagcg tgcctccgta tgcatggatg agagccccga tggcatggtg atactccccg
     4981 agtaccccca tcacctcctc tggctcgctg gtttccgcga acgacgtgaa gcctctgaga
     5041 tcggtgaaca ggacgacgat ttcgcggcga tggctgttca gcatgttctc ttcgccgacg
     5101 acgagatcgg ccagctgtgg cgacaggaag tggcgtagcc gattggtgcg ctccaactcg
     5161 gcaacctgtt gggtgacgcg cgattcgagt tcggtattcc aggccgccag ttcgtcggcc
     5221 tgacgccgga tggtgtcgtg gtattgcttg atccgcgcca gcgacgccac gcgcgcgagt
     5281 agctcgctct tgtcgaaggg tttggtgacg aagtcgtcgg cgcccgcctc gagggcggcc
     5341 agccgttgtt cggctccact ggcggtgatc atcacgacgg gcaggaattc cgttgcggga
     5401 gtggagcgta tcctccggca gacctcgtag ccgtccatct ccggcatcaa aatgtcgagc
     5461 agcacgatgt cgatgtcctc ggtctccagc agcgccagtg cttcagcacc cgatgccgcc
     5521 gtcacgaccc ggtggccgcg gggcgtgaga accgcgtcga gcagcctcaa attggtcggc
     5581 tgatcgtcga cggccaggac gatcgccttg atgtccatca ggtggcccgt ccgttgagaa
     5641 aagcttcgat ctgaccgggc agtgctcgcg cgctgatcgg cttctcgatg tagccgtcga
     5701 agccggcgcg agctgcccgc tccttgtcct cggccatcgc gagggccgtc acggcgacaa
     5761 ccggcacatc cgagccgagc gtgctctcgc gcaacctatg aagcgtctct gttccgtcga
     5821 taccaggcag ctggaggtcc atcaacacca gatccggggg agtctccttg gcggcgcgca
     5881 gagcttcctc acccgactgc gcctcgatga cgtcatagct ggcgaacgtg agcacgtcgc
     5941 gaaccagctt gagattcaac aggttatcct ccacgacgag gattcgcgcg ggcgtcacag
     6001 cacacctacc tgctgcagcg catccacgag ctcctcgcgg cgaattggct tgcgcagata
     6061 cacttttgcg cctagagcca gaccgcgggc ccgatcgtcg acgtctgttg cgatgaccac
     6121 cgggatagtg gcggtggcgg gatccgcctt gagctcggtc agcacctgcc aaccatcgag
     6181 tcgtggcagc ccgatctcca gcaccacggc ggcgggcagt tctttgcgtg ccaactcgag
     6241 agcttcgacg ccgtccatgg cccgcgttac gcgtatcggt gaaccatcaa gataggccga
     6301 gttgagatcg agggatgccc ggtcgtcatc gacgagaagg acaaccggca ggtgtctgga
     6361 cacatgcggc tcgggcgcgg gccgcagcat gcccggaaca gagaagccga acgtgcttcc
     6421 cttaccgggc gtgctctcca gccacattcg gccgccgaac aaccagacga tgcgacggga
     6481 gagcgtcagt ccgagcccgg tgccctcctc ttttggtgcg ccgcggcggc cctgctggaa
     6541 ggactcgaag attcgttcct ggtcttctgc cggtactccg atgccggtgt cggtcaccgt
     6601 caccaacagg tcggtcccct cgccgtaggc gcggacagtc acggtgccct tgtctggtgt
     6661 gaacttgacg gcgttggaca gcaggttgag cacgacgcgt ttgaacagca actcgtcgga
     6721 ctcgatcgtg tcgatcccgt cggcgacctc gacgccgatg gtgatggcgt gcgacgtcgc
     6781 gcgctcgcgg agcatcgccc ggcagtaatc aagggcactg ctgacgctga atgtgcttgg
     6841 ctcgagcacc atttggcccg cttcaacctt cgataggtcg agaatgtcgt tgacgagctc
     6901 cagtaggtgc ttgccggagt tccagatgtc gcgcaggtaa tcctcctgac gctcgttgat
     6961 ctcgccgaac atccgatcca gcagcacctc cgagaagccg atcaccgcgt tgagcggggt
     7021 cctcagctca tgggacatgc tggccaggaa ctctgacttg tgctggctgg cgatctcgag
     7081 ctcgctggtc ttggtctgca actccccgaa caagcgcgcg ttgacgatgg cgagggccga
     7141 ctgtccggcg aacgtctcga gcagttcgac atgcggtcca aaggagcccg tgctgcgacg
     7201 gcggatcacc agggcgccca ggatccgctc gccgcgcaga atcggaaccg cgagcaccga
     7261 ccgccagccg tcccggaaca acgcgtcgag gtggggatcg agctcggtct tggcggccag
     7321 gtccgggacc tcaactgtct ggtggtcggt tgcggcgcgt ccgaccagcg tcgtctcgcg
     7381 gtcaatcgtg atcgcccgca gccgcttcag aaggtcgggg ctgcttccgg cagcggcacg
     7441 gacgtggaac gaatcgctcg actcgtcgta ctccatgatg gatccgccgt cggttccggt
     7501 gagccgcacg gcgttgctga cgatctggtc cagtacctcg tcgaggtcga gcgtcgagcc
     7561 gacggcgtcg cccacctcgc ggagggcttc cagctgcttg agcttgctct tcagtgcgcg
     7621 cctcagcgcg tcctgtcgca tgacgatcgc gccctgaacg gcgaactcct cgaggagctc
     7681 gcactcccgg gcgttgaagg gcttgacgtt ggtgcgccac atggacagca cgccgacgac
     7741 ctcgttttgc agcatcatcg gcgtggacaa cagtgtgcgg aacccggcga gactctggag
     7801 gtcgaggcgt agatactccg cgtctttgaa cacgtccgga atctgaatgg tgcgcatctc
     7861 ttcggccgcg cgaccgacgg tggacgaccg gtttctggca agcggttggt ccagcaggag
     7921 cttgcgaaac tcctcgggga tcttgccggt atcgcgagac agccgaaaca cgtccccttc
     7981 agggatgtag agcgttgcgg cctgtgcacg gcagagatgc gcggcgcgct ccaggatggt
     8041 gtcgaggatg gcctcggggt cggcgtcggc acgccccaga gcggcgagga tctcgcggct
     8101 cgccgctgac tgctcgcggg catctcgcaa ctgctggttc agctccgtcg aggcccgtga
     8161 tacgcgggtg gtgcgctcgc ttctttcgcc caatactgcc tcccccggac cttttcagtc
     8221 tgtactgctt tcgctggccg gacagcggaa tggccgtgac agtttcgtca atttgcgcga
     8281 aggacggcga aaggctgccg atgagccact acaaagcaaa tgtgcgtgac cgtgaattca
     8341 acttgcgacg tccgcggaat gcatacgacg ctaatgggat acgcgagtgc cgccggtgag
     8401 cagccaactg aggtctaccg cgtgggattg cgggcagtgc cgttcctgct gtcggtcgcc
     8461 gacctgctga tcggctggct gttgttgtgg catgccgagg tcgcactcga cgcgggggca
     8521 tccgagcgcg acggcgcctt ctgccgcgga aagatcgctg cggcagtgct tttcgccgag
     8581 acggtgttgc cccgcctggc cgccgatcgg attgtcgtcg aatcgcagag cctggccgcg
     8641 atggaactcg acgaggaagc gttctgaagc tatgcgcggt gtggatccca tgcctgcggc
     8701 gagagcaact gcgcctgcca atggccagcc ttgatcagcc gcgcggcctc ttcgcgcagt
     8761 gtgttgccca acttcaccgt gaactcccgc ggcaactcca gctgcgacgt cgcggccaca
     8821 ccgagatgct gggtgagcgt gggctcgcgt agcggggcat aacgcatctg gcctcgctcg
     8881 atctcgcggc cacaggccga cagcggtagt acgccgtaca ccaggccggc ttcgatgagc
     8941 cgcttggtga cttgaagcga atcggtggtg acccggtagc cgatgttgac tttgactcgc
     9001 agcgcggcgt tttcgagcag agtgccgata ccggcgtggg agcgcggaat cacaagcggt
     9061 aacgcgacga gttcgatgaa cgtcagagcg cgaccggtgt cgaggtcgca ggcagggccg
     9121 ccgacgacaa ccagatcctc ggcgacgagg tcgcgataga aaacgcggtc atcgggcacc
     9181 gggttgatca gcgcgacgtc gaccgcccct ttgagcatcg cgtcgacgag tgcggcggtg
     9241 gtccccgcgg tgaccgagaa cgtgaggttc gagaacaccg tcgacaagct ggttatgagt
     9301 ggcgcggcga gcacgtcgac cgtcgtctcc ggcaggccca gccgaacgcc gcgctcgatc
     9361 ttcgccacgg gtgatccggc gtactgcaca gcgagttcga gctgccgcaa tggcgccgcc
     9421 gtcgaggcgc gcaaacgctc gccctcatcg gtcagctgca ctccccggcg ggttcggcga
     9481 aaaaggctga cgccgaggtc ctcctcgagc aggcgaatct ggcggctcaa cgccggttga
     9541 gcgacgccca gcgcagcggc tgcccgcgtg atcgagccct cctcggcgat ccgcaggaag
     9601 tacttcaacc ggtgagtgtc catcgccgcc cagatatacc agccataaat atgtggcatg
     9661 actgaacgtc gcaattggta ttaccgcgca ggtcgcacgc agtcctagtc tcgtatctga
     9721 gctcgacgga tgcccggctg atatggccaa gccatcgcgt cgcaggcgtg agaggagaag
     9781 cacgtgaccg tgcaggagga ccaggactgg gcgcaggtgc tggggttcga ccgattgccc
     9841 gagcacatcg tcggcaagcg cgtgaccgag ttgatggacc tgtcggggaa gcgggcgttc
     9901 gtgacggggg ccggcggcga cggtttgggt caagccatcg ccaatcgcct cgcaggcctg
     9961 ggcgcagacg tggcgttgat cggtcgcacc ctggaaaagg tcgagcgacg cgccaaggag
    10021 atcgagcaac gctggggtgt caatgcctac ccggtgagag ccgatatgtc cgactgggac
    10081 caggtgcacg acgcggtgcg cgaggcgcac ggcgagctcg gtggcctgga catcatggtc
    10141 aacaaccccg tgatggtgaa ggccgggccc ttcgagaagc agaccaaaga agacatcgac
    10201 tacaccgtcc ttggcagcct caccatgatg atgtacggcg cccatgccgc gctggaattc
    10261 cttctgcccc aagggtcggg caagatcatc aacatcggct cggtcggtgg ccgcatccag
    10321 cagcgggggc tggtcgtcta caacgcctgc aaatctggtg tcatcggatt tacccgcaat
    10381 ctcgctcatg aggtcgcact gcgcggcgtc aacgtgctgg gcgtggcgcc gggcatcatg
    10441 ctcaagccgg acatgaagcg ctacatgttg aacccgcaga acgatcaaga ccgagccggt
    10501 cgcggcgcgg tcgccgaggc gattactcaa caggtgcagc tgggccgcgc ctcgttgccg
    10561 gaggaggccg ccaacgtggt cgccttcctt gccaccgaag ccgccgatta tctgtgcggt
    10621 caaacgatcg acgtggccgg cgggcagtgg atgaactgaa acatgctcag tgtcaacgag
    10681 gttgaagacg cagctgccag caagaccgga ctgagtgact tcggggagcc ctcgtaccgc
    10741 gagggtctgg gcctcctact gaagtccttg ggcgcggagg cgcggctcaa cggccgcggt
    10801 gaggcgttca tctatcagcg catcatcggc tatctgggtc agcggctgca ggtggagaac
    10861 tggttccgcc ggcaccccga gatcgacgag cttccgatcg ccgcgccgct gatcgggctc
    10921 gggttacccc gcaccggttc gacggcgctg tcgatgctgc tcgcccagga ccccggcgtg
    10981 cggtacttgc ggaagtggga gtcgtcccag ccgtgcccgc cgccctccac ggtgcagggc
    11041 gtcgatccgc ggattccgcc ggacaagggc gaaatgatcg gcacgcgtta tcacgtgccg
    11101 accgacaccc acggtccgat ggagtgtcac gagctgatgg cgctggactt caagtcccat
    11161 ctgttccagt ccttcgcgca ggtccccagc tactcggaat ggctcatcga gaaggcggat
    11221 ctcgaaagca cgctgagcta ccagaaacgg gtgatgaagc tgctgcagtg gggcgaaccg
    11281 gcgaaaccgt ggcggctcaa gtgtccctct cacgtgctgt ggctcggcgc cgtgcactcc
    11341 gcctttccgg atgcgaaata cgtgatgaca catcgggacc cgaccgatgt catcctgtcg
    11401 gtggcagacc tgtacgccga catcatcgga tcgttcaccg acgacatcga ccgccgctac
    11461 atcgggcgac tcaacgtcga gcactggtcg ctgggaatgg atcgggcgct gcggttccgg
    11521 gccaacggtg ccgatgaccg gttctacgac atcgactttc gcgccatgca ggcggacccg
    11581 atcggcgagg tcaagggttt gtacgcgtgg ctgggtgagc cggtcggtga cgaattcgaa
    11641 agccggatgc agagctggtg gactcgggcc gctgcagagc gcgagcccag ctcgcacgcc
    11701 gacccggtcg aattcggcat cgaattggat gcggtgcgcc cgcgattcgc cacctacgtc
    11761 gaccacgcgg tccgctggac cgcccatcaa tccgcttaga gaaggacgtt catgtcgatt
    11821 gatctgaccg gcggcatcaa cccgtcgcgg gagtatgtgt tcgcagagcg cccagacaac
    11881 ccagagatgc gggattcggt gagtttctgg acggtggacg atcgtggcga gattggcttg
    11941 ccgcgcatcg gaatcgaggc tgtggccgcc aattgggaca accacgacat tcaggtcaac
    12001 gtcgcctttc cggatggacg ggtgtaccgg ctgcggggca acggcccgac attgcctgct
    12061 gaaggtcccg gtggtcagcc gaccgagctt ggtgcgggcg ggctggtctt tcggtgcctc
    12121 aagccattcg acacctggac gatgagctac gacggtaagg cggtgcagac atcatcggcc
    12181 gatctcgtgg cgggccgcac ggatggccct ctggttgacg tcgcgttcga ggtcaaggcg
    12241 acgatggctg tgccgccgtg ggtgcagggc gcgttgcagg ccgacgccgg gacgcaattg
    12301 aaaacgtccg tcgagggcga cctgatgggt ggaccgcggt atgagcaatt gttccgcgcg
    12361 gtcggctcgg tcaccgtcga caacggcgac gcacaacaat tcactggcag cggcctgcgg
    12421 atccgccgtc aaggcgtgcg caggctcgcc gggttctggg gtcactgctg gcaatccgcg
    12481 gtgtttccca gcgggaaggc gttcggctac atcgcctatc cgccgcggcc cgacgggcaa
    12541 ccgacgttca acgagggcta cctcttcacc ggtgacggcg cgttgattcc cgccacggtg
    12601 gtgcaggcgc cgtggctgac ccgattgcag gcgctgggcg aggatgtgtc actggttctc
    12661 gaaactaaca gcggcacagt ccaaatcgag ggcgagacgg tgttctcgac ccacgacatc
    12721 caccacaacg acgacacgta ctcggtacac gcgatgaaga acgagatacc ggactttccg
    12781 gcgttgcaac aggctggagt gcggtatcgg tgggacggcg aagaaaccta cggcatgctc
    12841 gaacgctcca accccctcga caagatctcg cgatgaacgg attggacggc aagagcttcg
    12901 tcatcgccgg cggagccacc ggcatcggcg cgggcaccgc caagcggctc gccgaggaag
    12961 gtgcctcggt cgtcgtcggt gacatcaaca tcggcggtgc caaggccacc gttgatgaca
    13021 tcgtgcggtc gggcggacga gccgtcgccg tcgagttcga cctcgccgac gaggcatcgg
    13081 tgcaacgtct cgtcgacgcg gccattgccg agttcggcgc ggtccacggg ctgcacaacg
    13141 tcggcgccga cctgtccgag aacaacctgg gccgcgataa gacgttgctg gacaccggtt
    13201 tcgatgtctg gcatcgcacg ctcgacgtta acctgctcgg ctacgtccgc acgatccgtg
    13261 cagtcttacc gcacttgctc gagcgcggcg gcggcaacat cgtcaacacg tcctcgggcg
    13321 gctcactggg cagcgacccc agtcacgtcg cctactgcgc ctccaaggca gccgtcaacc
    13381 acctcactcg ccacgtcgcc aacaactggg gcaagcaggg catccggtgc aacggagtga
    13441 tgccggggct ggtgatgggg gagacgcagg agcggcacaa cgaccaacag atccaacaga
    13501 tgttcctgat ggccgcgaag gtcacgcgcc ttgggaaacc gagcgacctc gccgcgatta
    13561 ccgccttcct cctatcggat gacgccgaat ggatcaacgg tcaggtgtgg tacatcggcg
    13621 gcgcatccca catgcggcaa tagcgattac ggacacaaga acgccaaggg cgggcacggg
    13681 ccgtaaaagc gcggcggagg agggtcagga aacccttcga tgattgtcga attggtcaga
    13741 ggactgttcg ggtcgaagta ccacggatgg cacacgttca tgtcccagtt aggcatgttc
    13801 cgcattaccg gtgagcccgg acaccactga taggtgcaga agttttgggg acccggcggc
    13861 gagttcggat cttggcacac catcggggcc ggctgggcct gggcggtacc cgcggccagc
    13921 ccgaaaccag ctagacccag gccaccagat accaacgcag ctgttgccaa ccccgcgaac
    13981 ggcagtttcg tgagcttcat cgctttcctt ccgttcccgg tgtccagtag tggcccggtc
    14041 acgaaaaggt tgcgtcgacg tccttggcga attcttggaa gctgcgcgcg gtcctggatg
    14101 tcaccacgtg acgggcaact ccttcatccc gtagatgccc gattcgccct tgaactggag
    14161 catgtccaga gcagcgttca gtcggagccc aggcaggcgc cgcgccagcg tcgcgaacgc
    14221 gatctgcatt tcgactcgcg cgagattctg cccgatgcac tgatgcaccc cgtagccgaa
    14281 acccacgtgt ccgcgggtgt ttcggtcgat gtcgagggtt tcggggtcat cgacgaagtc
    14341 gggatcccag tttcccgcgg gcaggttcat cacgagtagg tcgccagcgt gaaccaactg
    14401 gccgccaatc gtgaggtctt cggtcgccag acgatccacc tggctgtgca cgatggtgag
    14461 gtagcgcatg agctcttcga cggcgttcgc gatgacggcc tggtcatcgg tctgtccgag
    14521 tcgctcgaac acatcgcgat gctgcaccag cgcaaccgct cccagtgcga tcatgttcgc
    14581 ggtggtctcg tggcccgcct gcatcatgat cgcgcccgtg accgctgcgg tctcacgggt
    14641 gagctggccg gttgccacgt actcggtggc aagccggctc atcaagtcgt cgcccgggtt
    14701 gtgctccttg aggtcgacca actcccgtat ataggtgcac atcgctccga acgcctgcgc
    14761 cttttgttcg tcggacgacc tggtatcgag ggagaccgtg gtgttgcgat ggaagtactc
    14821 gagatcgtcc gacggcacac cgagtaaaag tgcaatcacg agtgacggca caggcaaagc
    14881 gaagtcgcgc acgaggtcgg aaggcgggcc gttgtcgatc atttcgtcga gatagcggtc
    14941 gacaatccct tgaatctgcg ggcgcatact gtcggctcgt cgaaacgtga agtcgcgcgt
    15001 catcatgcgc cgcaagcgat ggtgctcggg gtcgtcgatc cgcgcgaaca tcaccggcgt
    15061 ctcgccatct gctgtcgccg gcatgatcga cgccgggatg gtttctgcgg acaggcgcgg
    15121 atcgaccagc gcggctctga tgtcgtggta gcggctcacc acccacgtgg gctgcccctg
    15181 atacatcgcc cgtcgcaagc cgggttcctc ccgccacttc gcgaactccg ccggagggtg
    15241 cagcgggcag cttgccgcgc gctccgcggg aatgacgaga aggggcgtct cagatttggc
    15301 atcggaagtc gttggaatca ccggcgtttc cccataagtg tcaggtgcct gtcaattagt
    15361 cgagcctaga tcccgacaaa gtggcagtca accgtcagtt acgctgtcag gatgactgcg
    15421 gtggccgacc ggccgttgcg agcggatgcg gcgcgcaacg ccgagcgcat tctgcgcgcc
    15481 gcgcgcgagg cctacggcga actagggccg gaggcgccgg tggaggccat cgctcgtcgt
    15541 gccggtgtcg gcgaacggac gctgtaccga aggtttccga cgaaggccga cctgatcagc
    15601 gccgcgctcg agcagagcat cgcggaggat ctgactccca ccatcgagga tgcgcgccgc
    15661 gccaccgacc cgctgcaggg cctcgcccag ctgatcgagg cagcgatatc gctcggcgca
    15721 cgcgaacacc acctcttgac cgcggcgcgc agggccggat cgctcaccat ggacatttcg
    15781 accccgatgt atgacgtgct caccgaactc gccgaccgag cccaggaggc gggcctcgtc
    15841 cgtgccgatc tggtcgccga cgacctgccg cgcctcatcg cgatgcttta cagcgtgctc
    15901 tccactatgg actcaaccgg tgatggctgg cggcgctacg tcgccctgat tctcgacgcg
    15961 ataacggtca acgactcccg accgctgccc ccggcggtcg ctcttcgcta ccaacctctc
    16021 acgagcacct ggccgtagct tctcagtagc cattcggccc gcatggccgc ggaatgaaca
    16081 gagaccagca catgttgggg gcctgccacg ggtacggagg cgggggattg tcgccgtccc
    16141 aaacagtgga agaaacgttg ccctgcccag gattgacgta gtgataggta tggcagacgt
    16201 tccagtccca attcggccgc gcgtccgcgg gcgtgttgaa cccgccgcca tagcccccag
    16261 cctcatcccc aggacaccac tgataggggc cgccggcgtg ggcgatacct gcaccgagcc
    16321 ccaaactggc caggcccaaa ccgccggaca ccagcacggt cgtcgttaat cgcgaaatcc
    16381 ggttcatggt tgcgtccttt cgtgaaccgc caccccgttg gcgttgccaa tgcgattgag
    16441 tacagaccct ccagcgtctc taccgatccc aaccttgcga ccgatgcctt ggcggattct
    16501 tggaacggcg tgcaaccagg tagagctagt ggtcgacggt gatgtaggac ttgcggatgg
    16561 tctcgcggac cacccaggag tcacgccatc ctcgcggcag gacgcgcagg gagcctgggc
    16621 cgagagacac tcgttgtcct tcttggtcgg ccaccggttc aacgcccggt gatcatgttg
    16681 gctatgcgtg caattcggct ggtatgcgcg aggttcttct cggtctcccg ccggtcggcc
    16741 atgcggtaca acacatacat gctttgcacg ccaagccatc tcagcggctc gggttcccag
    16801 cgcctgacat ggcggccaac ccaggacaaa cgggtgagct cagtgtcgcg ttcgagcacc
    16861 aagtcggcga gggtgcgacc ggcaaggttg gtggtggtca gcccgctgcc gacgtagccg
    16921 cccgccgtgc ccagccccgt gctgcggtcg agatcgacgg tggccgtcca gtcccgcggc
    16981 acgccaagga cgccgcacca cgcatgcacg atcggcacat cggtggtggc tgggaacatg
    17041 tcgtgcatca acgccgtcaa ggcttcgact gtccagtgct gcgtggcacc tcggtcgtcg
    17101 atccgcgatc cgtagcggta cgggatgcca cggccgccga gagcgatgcg gttgtccgcg
    17161 gtgcgctgcg cgtacatgta gccgtgcgcg tagtcgccga gcagttcggc gtcctgccag
    17221 ccgatctggt catacgcgtc cttcggcagc ggcgcggtga cgaccatcga ggagttcatc
    17281 gggagccaat tacgccgctg gccttcgata ctcgcggtga atccctccag gcaacgcagg
    17341 acgatgggtg cgcgcacggt gccgaggtct gtcaccgccc gaccctgttc gatccggttg
    17401 accgcggtct gctcgaagat cgtcacaccg gtggcctcga cgacgcgggc cagcccctgc
    17461 accagtttgg caggctgaac ccgggcgcag tgggggctga aaaggccgcc gagcgcgcct
    17521 gcgactcgga tacgactgtc catctcgtcg cggctcagcg ccacgaagtc ctccggcccc
    17581 gcgccccaat cttgttcgta ccgcagggat tcgtgcaacc ggcccagctg agcacggttg
    17641 cgggcgacgt ggagcagccc gtccttggcg atgtcggcgt cgatgccctc gcccttcgcg
    17701 acggcgatga cctcgtcgac tgcgccgcgc atggcgcgca tgaggtcgac gacaccctgg
    17761 tggccgtgtg tcgcggcgta ggcatcgcgt ttgcctgcca attcggccga cagccaaccg
    17821 ccgttgcgac ccgaggcgcc gaagccggcg aactcgcggt ccaacaggac gatccgaaga
    17881 tcgggctgcg cctgcttcag gtagtaggcg gtccacagac ccgtgaaccc ggcgccgacg
    17941 atgcacacgt cagcgtcgcg atcgcccggc aatggcggcc gccggtcggg cagaccgatc
    18001 gagcggtacc agaaggacac gtcgccgtta acgaattcgg ttggtgcatc gacggggttc
    18061 tcgtcgatgg acatggttgt gctcctgttc gttatgcgag gatcgcggcg gcgtctgggc
    18121 ggaatgtgag ccagactcga tcgtcggcgt cggaagggct aagggcgcgt gaggacggat
    18181 gccgtcgtca tcgatttggg ctttaaggac tccttaactg gtgggcgggc tgacgatcca
    18241 gagaagctcg gcgcgttcgt cgccggtgtt gtgcaccgtg tgggggatgg acgtgcggta
    18301 ctcggcgctg tctcccgcgt cgaggcgatg ctttgcgccg tcgagttcga ggaccacgct
    18361 gcccgagatg accaggagga tctcctggga atcgtcgtgg acgtaggcct caccggcggt
    18421 cccgcccgga gcgaactccg cgacgtagac ttcgaggttg cgtagcggct tctgggagag
    18481 caggtacttg gcgctggtgt ccgaaacatg gatctcggga cggtcgtgct tgcgcaggat
    18541 gcggggaccg cttgcggggt ggtctgtgaa caggtcgggg agggtgatcc cgaggccctc
    18601 gcacaggcgg cgcagggtcc ccacgctcgc gttgacttgc cctcgctcca actgactgag
    18661 gaagccgggc gatgtcgcgg cccgaacggc gagctcgcgc agggtcagct tgcgtgcctg
    18721 ccgaaactcg cggatgcgcg cacccattgc cgattcagcg gtcaacggga cggaatcctc
    18781 ggggctctcc atctatcgaa cgtccgttcg tgaaagaaga tcacattttc tgtgacaa
//
LOCUS       DASRJA010000099         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_199168,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000099 DASRJA010000000
VERSION     DASRJA010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199168"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /gene="rpsA"
                     /locus_tag="VFC01_04545"
     CDS             <1..264
                     /gene="rpsA"
                     /locus_tag="VFC01_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216146.1"
                     /note="in Escherichia coli this protein is involved in
                     binding to the leader sequence of mRNAs and is itself
                     bound to the 30S subunit; autoregulates expression via a
                     C-terminal domain; in most gram negative organisms this
                     protein is composed of 6 repeats of the S1 domain while in
                     gram positive there are 4 repeats; the S1 nucleic
                     acid-binding domain is found associated with other
                     proteins; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZN78950.1"
                     /translation="GFDSETNEWLEGFEKQREEWESRYAEAERRHKMHTAQMEKFAAA
                     EAEEAAKPSSSNGASRSEEPAAGGSLASDAQLAALREKLAGSA"
     gene            274..1434
                     /gene="coaE"
                     /locus_tag="VFC01_04550"
     CDS             274..1434
                     /gene="coaE"
                     /locus_tag="VFC01_04550"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZN78951.1"
                     /translation="MIRIGLTGGIGAGKSTVAATFAECGGIIVDGDVIAREVVEPGTE
                     GLAQLVDAFGEDILLPDGALNRPALAAKAFVDDEKRAVLNGIVHPLVGKRRQEIIDAV
                     HEDAVVVEDIPLLVETGMAPAFPLVVVVTANEETRVERLIKRGMDEADARARIKAQAP
                     EEKRRAIADVLLDNSGSQGALVGKARDLWYNRVLPLAHNIRTRECASTVYQLVPYRPD
                     WLDDARRIIKRLQMACGEKALRIDHIGSTAVEGMDAKDVIDIQITVASLDVADEINDA
                     LANVGYPRHDHITADWPHTDDPSVWRKRFHCAADPGRSANLHIRVDGWPGQQFALLFR
                     DWLRANPGVQDDYLAAKRRALSAPDYAEAKEPWFADAYQRAWEWADATGWEP"
     gene            1628..2213
                     /locus_tag="VFC01_04555"
                     /pseudo
     CDS             1628..2213
                     /locus_tag="VFC01_04555"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
     gene            2400..>2740
                     /locus_tag="VFC01_04560"
     CDS             2400..>2740
                     /locus_tag="VFC01_04560"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HZN78952.1"
                     /translation="MSSAVCNLATHLDNPAERLATIRASMRYNTQFLRRLPRQVSLYL
                     AGVVGMPFNDGSRMLQFNVGISHVADARKALYHNGARLEGTYGFPPTLRGHALNIGLA
                     SNAVSLNFAIVG"
BASE COUNT          481 a          885 c          904 g          470 t
ORIGIN      
        1 ggcttcgact ccgagaccaa cgaatggctc gagggcttcg agaagcagcg tgaggagtgg
       61 gagtcccgct acgccgaggc ggagcgccga cacaagatgc acaccgcgca gatggagaag
      121 ttcgccgctg ccgaggccga ggaggccgcc aagccctcgt cctccaacgg cgcatcgcgc
      181 tccgaagagc ccgcagccgg tggatcgctc gcgagcgatg cccagctggc ggcgctgcgg
      241 gagaagctgg ccggcagcgc ctaaccggcc tgcatgattc gcatcggttt gaccggcggc
      301 atcggcgccg gtaagtcgac ggtggccgcc acattcgctg agtgtggcgg catcatcgtc
      361 gatggggacg tcatcgctcg cgaggtcgtc gaacccggaa ccgaggggct ggcgcagctg
      421 gtcgacgcgt tcggcgagga catcctgttg cctgatggtg cgctgaaccg gcccgcgctg
      481 gcggccaagg cattcgtcga cgacgagaag cgggccgtgc tcaacggcat cgtgcacccg
      541 ttggtcggca agcgtcggca ggagatcatc gacgcggtgc acgaagatgc cgtggtcgtg
      601 gaggacatcc cgctgctcgt ggaaaccggg atggcgccgg cctttccgct cgtcgtggtg
      661 gtcaccgcca acgaggagac tcgagtcgag cggctgatca agcggggaat ggacgaggcc
      721 gatgcccgcg cccgcatcaa ggcgcaggcg cccgaggaga agcgccgcgc aatagccgat
      781 gtgctgctgg acaattcggg ctctcaaggg gcgctcgtcg gaaaggcccg cgacctctgg
      841 tacaaccggg tgctgccgct ggcgcacaac atccgcacgc gagaatgcgc ctcgacggtc
      901 taccagctcg tgccgtaccg cccggattgg ctcgatgacg cgcgccggat catcaaacgt
      961 ctccaaatgg cctgcggcga aaaggctttg cgaatcgatc acatcggctc gaccgcagtc
     1021 gaaggcatgg acgccaagga cgtcatcgac attcagatca ctgtcgcatc actggacgtc
     1081 gccgatgaga tcaacgatgc cctggccaat gtcggctatc cccgccacga ccacatcacc
     1141 gcggactggc cgcacaccga cgatccgtcg gtgtggcgca agcggtttca ctgcgcggcc
     1201 gatcctgggc ggtcagccaa ccttcacatc agagtcgacg gatggccggg tcagcagttc
     1261 gcgctgctgt ttcgcgactg gttgcgggcg aaccccggtg tccaggacga ctacctggcg
     1321 gccaagcgtc gcgcgttatc agcccctgac tacgccgagg ccaaggagcc atggttcgcc
     1381 gacgcgtacc agcgcgcgtg ggaatgggcc gatgccaccg gttgggaacc ctgacctaca
     1441 ttgtttcgtt cgctgaactt tctatgaaaa tttgccagca cgggcgccaa accgccatta
     1501 actgacgctg ccgtcagtac catccgcgtt catgggcgtt cggttcaagc ggttcacgcc
     1561 gcccgacgcg atgacgttcc tgctgatgga ctcgcgcagg cgccccatgc acatgggcgg
     1621 gttgcagatg ttcgcccggc cggagggggc agacgacgac ttcgtccggc gcacctatga
     1681 agagatgcgc acctgtctgg acgtcgcgcc gatgttcgcc gggcatccca gcaccgttcg
     1741 cgggcgccca ttcggatgtc gacctcgatt accacctgag gtacacgacg ctgccggccc
     1801 ctggcaggga gcccgaactg tttcaactgg tttccgactt gcacggtcgt cgcctcgacc
     1861 gacgtagacc gttgtgggaa gttcacgtca tcgacggtct ggatgacggg cgcttcgcga
     1921 tcttcaccaa ggcccatcac gcactgttcg acggtgtgtc cttcctgagt ctcctacgac
     1981 ggtcactgag caccgacccg cacgatcgca cggtccgggc agtgtggtcg caacggccca
     2041 agcccgcaac cgaatcgcaa cgacgtccgg tgcccggtgc ctcgttgacg gaaactgtgg
     2101 gcctgatcag ggatgcgtgg cgtgagcgcg aactgttccc ggtcttccgc gcgccgagca
     2161 cctttctcaa tgtccgcagc gaggtgccgt gggtttgtgc catcgagcgg tggccgattg
     2221 agcgggtaga ggccgtcgca ggcgcggccg aggcgagcgt caacgatgtc gctctggcga
     2281 tgtgtgccgg tgcgcttcgc tcgctgctcc tcgaccatga cgcattaccc gaccgtccgc
     2341 ttgtcgcctt gagtcccgtg gatctgcgga cggccggaga cgtcgaaggc cgcaacgtca
     2401 tgagtagcgc ggtgtgcaat ctcgccactc acctggacaa cccggcggag cgcctggcga
     2461 ccatccgcgc gtcgatgcgc tacaacacgc agttcctgag gcggttgccg cggcaggtgt
     2521 cgctgtacct tgcaggcgtc gtcggaatgc cgttcaacga cggctcgcgg atgctgcagt
     2581 tcaatgtcgg catttcccac gtcgcggatg ccaggaaagc cctgtatcac aacggcgcac
     2641 ggctcgaggg gacgtacggc ttcccgccga cgctgcgcgg acacgcgttg aacatcggac
     2701 tggccagcaa tgccgtcagc ctgaactttg cgatcgtcgg
//
LOCUS       DASRJA010000100         9357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2739624,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000100 DASRJA010000000
VERSION     DASRJA010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2739624"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2141
                     /locus_tag="VFC01_04565"
     CDS             <1..2141
                     /locus_tag="VFC01_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZN78953.1"
                     /translation="REVGVGQVLAITFTNKAAAEMRERVVNLIGPRARNMWVSTFHST
                     CVRILRNQASLLPGLNSNFSIYDADDSRRLLLMIGKDMGLDTKRYSPRLLANGISNLK
                     NELIGPEEAAHDAANAMEDLPRIIAEVFAEYQRRLRAANALDFDDLIGETVAVLQAFP
                     QIAQYYRRRFRHILVDEYQDTNHAQYVLVRELVGRETEDGVAPAELCVVGDADQSIYA
                     FRGATIRNIEDFERDFPNATTILLEQNYRSTQNILSAANSVISRNAGRREKRLWTDAG
                     EGELIVGYVADNEHDEARFVAEEIDALADNGEITYNDVAVFYRTNNSSRALEEVFIRA
                     GIPYKVVGGVRFYERREIRDIVAYLRVLDNPGDSVSMRRILNTPRRGIGDRAEACVAV
                     YAENTGLSFNDALQAAAEGKVPMLNTRSEKCIASFVELIDELRGRLDGELGELVEAVL
                     DRTGYRTALESSSDPQDLARLDNLNELVSVAHEFSTDLANAQALGEVDPQDEDIPDTG
                     VLAAFLERVSLVADADELPEHGAGVVTMMTLHTAKGLEFPVVFVTGWEDGMFPHMRAL
                     GDPTELSEERRLAYVGITRARQRLYVSRARVRSSWGQPMLNPESRFLKEIPQELIDWR
                     RTEPAPSLSAPVSGAGRFGAPRPSPTRSAAGKRPLVVLKPGDRVTHDKYGLGRVEEVS
                     GVGESAMSLIDFGSAGRVKLMHNHAPISKL"
     gene            complement(2138..2689)
                     /locus_tag="VFC01_04570"
     CDS             complement(2138..2689)
                     /locus_tag="VFC01_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78954.1"
                     /translation="MPAESEPQTSIIRRHPTGEIPAAVAEPPTWGHLPPEGQGDYIPR
                     ARPVAVGTPRSKRAHPRAAIATAIACILSGWATAVIATDLITGWWQSDRLFCVAIGFL
                     TAVSAAALISGLIALLLRRRMGRLLIVVGAVVALLIFAGLFVAGAKLSPVVYTIPVLP
                     VASVLLAMLPATRRWARSGDLRV"
     gene            complement(2776..3816)
                     /locus_tag="VFC01_04575"
     CDS             complement(2776..3816)
                     /locus_tag="VFC01_04575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZN78955.1"
                     /translation="MTTKPRRTWADLEAAIAAAEITDIIPFNEFGDLTDLDFRENCAF
                     DRESQVVHAPELDDLHDTDDLVPLRLAVPSEFRANAGDERQLSHAYRDSHTDTSNGVA
                     ATDVIDISGRRGLHRKQDVPVKGRLMVAAMAVGATAAGAYTMTHATDTTADDTVLAAD
                     KSVTGGTALTGSVDGMQIVSVAPAALETVHAEEITKAAAFAQERAEREARLNRPLFVM
                     YTKGVFTSGFGYRWGVLHGGIDIANSIGTPILAVADGVVIDAGPTAGYGAWVKLRHAD
                     GTVTLYGHVNTWLVSKGDRVMAGDQIATMGNRGNSTGPHCHFEVLLNGTNRIDPVPWL
                     AQRGIGPGNYVG"
     gene            4102..5277
                     /gene="sucC"
                     /locus_tag="VFC01_04580"
     CDS             4102..5277
                     /gene="sucC"
                     /locus_tag="VFC01_04580"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZN78956.1"
                     /translation="MDLFEYQAKELFAKHNVPTTPGRVTDTAEEAKAIATEIGRPVMV
                     KAQVKTGGRGKAGGVKYAATPDDAFTHAQNILGLDIKGHIVKKLLVSEASDIAEEYYI
                     SFLLDRANRTYLAMCSVEGGMEIEEVAATKPERLAKVPVDAVKGVDLAFARSIAEQGH
                     LPAEVLDAAAVTIQKLWELFVAEDATLVEVNPLVRTPDDQILALDGKVTLDANADFRQ
                     PGHAEFEDRDATDPLELKAKANDLNYVKLDGEVGIIGNGAGLVMSTLDVVAYAGEKHG
                     GVKPANFLDIGGGASAEVMAAGLDVILHDPQVKSVFVNVFGGITACDAVANGIVTALK
                     ILGDEANKPLVVRLDGNNVDEGRRILAEANHPLVTEVATMDEAADVAAAKAAEAANA"
     gene            5288..6190
                     /gene="sucD"
                     /locus_tag="VFC01_04585"
     CDS             5288..6190
                     /gene="sucD"
                     /locus_tag="VFC01_04585"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZN78957.1"
                     /translation="MAIFLTKDNKVIVQGITGAEATTHTARMLKAGTQIVGGVNARKA
                     GTTVSHKDVNDNDIELPVFGTVVEAIEKTGADVSIIFVPPKFAKDAIIEAIDAEIPLL
                     VVITEGIPVQDSAYAWAYNVEKGKKTRIIGPNCPGIITPGQALVGITPANISGAGPIG
                     LVSKSGTLTYQMMFELRDFGFSTSIGIGGDPVIGTTHIDAIEAFEKDPGTKVIVMIGE
                     IGGDAEERAADYIKANVSKPVVGYVAGFTAPEGKTMGHAGAIVSGSSGTAAAKKEALE
                     AAGVKVGKTPSETARLAREILQSL"
     gene            6361..7887
                     /locus_tag="VFC01_04590"
     CDS             6361..7887
                     /locus_tag="VFC01_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HZN78958.1"
                     /translation="MVDPRTPVIVGVGQFTERIHDADYRGMSAVELATQAARAALADT
                     GVDNAKIASAIDTVFALRQFEISGPAPAKLGRSNNYPRSVMNRLGGDPARVVLEPVGG
                     QSPQKLVTEAGNTIVSGDADVVMIMGSEPGSTARYFANRDDKPDFTEHVDGQLEDRGH
                     QIYTYIDDYTIQHGLTGAPVQYGLLENARRARLGLNVTDYRRRMAELFAPFSKVAAKN
                     PFSSSPVERSVDEILTVTDDNRMICDPYPRLLVARDQVNQGAAAVMMSVEAARRLGVA
                     EDKWVYVHGHADLVEQATLDRADLGAGPATVMAAREALRVAGIGVDDIATFDLYSCFP
                     FPVFLISEALGIDGDDPRGLTLTGGLPYFGGPGNSYSLHGIAETVIEMREAPGQFGFV
                     GANGGIMSKYSVGIYSTEPVEWRTDRSEELKQEIAALPKVPVSRNPDGAATIETYSVR
                     YDWPIRTGIIVGRLDADNSRFMATTDDEDLVALMSDGDPLGAAISVWSTEHGNRASLA
                     "
     gene            complement(7884..8726)
                     /locus_tag="VFC01_04595"
     CDS             complement(7884..8726)
                     /locus_tag="VFC01_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014981197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN78959.1"
                     /translation="MERMIEANGVELCTEPFGDPGDPPILLVMGTGASMLWWDEGFCR
                     MLAERGRFVIRYDHRDTGRSITYEPGRPGYTSADLVADAAGVMDAYRVPAAHVVGVSA
                     GGAIAQLLALDFPDRVRSLVLISTSSALPGERELPPPTEDFTRFVTTAEVDWSDSESV
                     IEYLVDYSRVLAGAMRPFDEGPMRVLVRRDVERAHDFAAAQNHDVLPDGEPPRAPLSS
                     ISVPTLVIHGSADPMFPVAHGEALADAIPGAKLLPLDGSGHGVDRADWETIVGAIVAH
                     TAAG"
     gene            complement(8764..>9357)
                     /locus_tag="VFC01_04600"
     CDS             complement(8764..>9357)
                     /locus_tag="VFC01_04600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZN78960.1"
                     /translation="VALPVEHDPITLAKSIATLDHLSGGRVSVGVGFGWNTDELADHN
                     VPPARRRTMLREYLEAMRALWTQEEASYEGEFVNFGPSWAWPKPIQSHIPVLVGAAGT
                     EKNFKWIARSADGWITTPRDFDIDEPVKLLQDTWAAAGRDGAPQIVALDFKPDPDKLA
                     RWADLGVTEVLFGVPDKAEAEVASYVERLAGKLAALV"
BASE COUNT         1674 a         3105 c         3104 g         1474 t
ORIGIN      
        1 cccgtgaggt cggcgtcggg caggtgctgg ccatcacgtt caccaacaag gcggccgccg
       61 agatgcgcga gcgggtggtc aacttgatcg gcccgcgcgc ccggaacatg tgggtgtcga
      121 cgttccactc cacgtgcgtg cggatcctgc gcaaccaggc ctcgctgctg cccgggctga
      181 attccaactt ctcgatctac gacgccgacg actcgcggcg gctgctgctg atgatcggca
      241 aggacatggg gctggacacc aagcggtact cgccgcggtt gctcgccaac ggcatctcca
      301 acctcaagaa cgagctgatc gggcccgagg aggcggcaca cgacgcggcc aacgcgatgg
      361 aggatctgcc gcgcatcatc gcggaagtct tcgccgagta tcagcgtcgg ctgcgtgcgg
      421 ccaacgcgct cgatttcgac gacctcatcg gcgagacagt cgctgtgctg caagcctttc
      481 cgcagatcgc gcagtactac cgcaggcggt tccgacacat cctggtcgat gagtaccagg
      541 acactaacca cgcccagtac gtgttggtgc gcgaactcgt cgggcgggaa accgaggacg
      601 gcgttgcgcc cgccgagctg tgcgtggtgg gcgacgccga ccagtcgatc tacgcgttcc
      661 gcggcgcgac gattcgcaac atcgaagact tcgaacgcga cttccccaac gccacaacga
      721 ttctgctgga acagaactac cggtccacgc agaacatctt gagcgcggcg aactcggtga
      781 tctcccgcaa cgcggggcgg cgggagaagc ggctgtggac cgacgccggc gagggcgaac
      841 tgatcgtcgg ctacgtcgcc gacaacgagc acgacgaggc acgcttcgtc gccgaggaga
      901 tcgacgcgct cgccgacaac ggcgagatca cctacaacga cgtcgcggtg ttctaccgga
      961 ccaacaactc gtcgcgcgct ttggaagaag tgttcatccg ggccggtatc ccgtacaaag
     1021 tcgttggcgg cgttcggttt tacgagcgcc gggagattcg cgacattgtc gcctacctgc
     1081 gcgtgctgga caaccccggc gactcggtga gcatgcggcg catcctgaac acgccacggc
     1141 gcggcatcgg cgaccgtgcc gaagcgtgcg tggcggtgta tgcggagaac accgggctga
     1201 gcttcaacga cgcgctgcag gccgccgccg agggcaaggt gccgatgctg aatacccgct
     1261 cggagaagtg cattgcgagt ttcgttgagc tgatcgacga gctgcgcggc cgtctcgacg
     1321 gtgagctcgg tgagctcgtc gaggcggtac tcgaccgcac cggctaccgc accgcactgg
     1381 aatcctccag cgatccgcag gatctggcac gcctggacaa cctcaacgaa ttggtcagcg
     1441 tcgcacacga attcagcacc gacctggcca acgcgcaagc gctcggcgag gtcgatccgc
     1501 aagacgagga cattcccgac accggcgtgc tggcggcgtt tctggagcgg gtgtcgctgg
     1561 tggccgacgc cgacgagctg cccgagcacg gcgccggcgt ggtgaccatg atgacactgc
     1621 acaccgccaa gggtctggag ttcccggtgg tgttcgtgac gggctgggag gacggcatgt
     1681 tcccgcacat gcgggcgctg ggcgatccga ccgagctgtc cgaggagcga cggctggcct
     1741 acgtcggcat cacccgggcc cggcagcggc tctacgtcag ccgcgccagg gtccgctcgt
     1801 catggggcca gccgatgctc aacccggaat cgcggttcct gaaggagatt ccgcaggagc
     1861 tcatcgattg gcgccgcacc gagccggcac cgtcgctttc agctccggtt agcggcgcgg
     1921 gccggttcgg cgcgccacgc ccgtcaccga cgcgctcagc ggccggtaaa cgcccgctgg
     1981 tggtgctgaa gccgggcgat cgcgtgactc acgacaagta cggcctcggc cgtgtcgaag
     2041 aagtgtccgg tgttggcgag tcggcgatgt cgctgatcga cttcggcagc gccgggcggg
     2101 tcaagttgat gcacaaccac gccccgatct ccaagctcta aacccgcaga tccccgctgc
     2161 gggcccaccg tcgggtggcc ggcagcattg cgagcagaac gctggccacg ggcagcacgg
     2221 ggatcgtgta gaccaccggc gaaagtttcg ctccggcgac gaacaggccc gcgaagatca
     2281 gcagcgccac gaccgcaccc accacgatca gcagcctgcc catccgccgc cgcagcagta
     2341 gcgcgatcag gccgctgatg agtgccgccg cggacaccgc ggtgagaaag ccgatggcca
     2401 cgcagaacag ccggtccgac tgccaccagc ccgtgatcag gtcggtggcg atgaccgcgg
     2461 tggcccagcc ggacaggatg cacgcaatcg ccgtggcgat cgccgccctc ggatgggccc
     2521 gcttcgagcg cggtgtgccc accgcgacgg gtcgggcgcg cgggatgtag tccccctgcc
     2581 cttcgggcgg gaggtgcccc caggtcgggg gctcggctac cgcggcaggg atctcaccgg
     2641 tcggatgacg ccgaatgatg ctggtctgcg gttcggactc ggccggcatc ggcgatgacg
     2701 gcgtgccatc gtcggagttt cgtcggatgc gtggttgatc tacaggcggt tggtcggacg
     2761 ggccgtcact cacatctagc caacgtagtt gccggggcca attccccgct gcgcaagcca
     2821 cggcaccggg tcgatccggt tggtgccgtt gagcagaacc tcgaagtggc agtgcgggcc
     2881 ggtggagttg ccgcggttgc ccatcgtcgc aatctgatcg cccgccatca cccggtcgcc
     2941 cttgctgacc agccaggtat tgacgtggcc gtacagcgtg acagtgccgt ccgcgtgccg
     3001 cagcttgacc catgcgccgt agccggcggt cgggcccgcg tcgatcacca cgccgtcggc
     3061 gacggcgagg atcggggtgc cgatcgagtt ggcgatgtcg ataccgccgt gcagcactcc
     3121 ccagcggtag ccgaagccgg aggtaaagac gccctttgtg tacatcacga acagcggccg
     3181 gttcaggcgc gcttcgcgct cggcgcgctc ctgtgcgaat gcggcggcct tggtgatctc
     3241 ctcggcgtgc acggtctcca gggccgccgg ggcgacggag acgatctgca tgccgtccac
     3301 cgagccggtg agcgccgtgc cgcccgtcac ggacttatcg gcggccagca ccgtgtcgtc
     3361 ggccgtcgtg tcggtcgcgt gcgtcatcgt gtacgcgccc gccgcggtcg cgccgacggc
     3421 catcgcggcg accatcagcc ggcccttgac gggcacgtcc tgcttgcggt gcaggccgcg
     3481 gcggccggag atgtcgatga cgtcggtggc ggcgacgccg ttactggtgt cggtgtggct
     3541 gtcgcggtag gcgtgcgaca gctgacgctc gtcgcccgcg ttcgcgcgga actccgaggg
     3601 caccgccagc cgcagcggca ctagatcgtc ggtgtcgtgc aggtcatcga gctcgggcgc
     3661 gtggacgacc tgcgattcgc ggtcgaacgc gcaattctca cgaaagtcca ggtcagtgag
     3721 gtcgccgaac tcgttgaacg gaatgatgtc ggtgatttcc gcggcggcga ttgcggcttc
     3781 gaggtctgcc caggtgcgcc gtggtttcgt cgtcactccg tcgggagatc gagacgaacg
     3841 gtgctgcgcc aacctgaaat atccttctag tcgtgaccga accgtgatct agaccagagg
     3901 cacgttaacc aaatcccaat gttcgtcgca acccgaggga gtattccggc cggggattgt
     3961 gacctgtatc accctcggtc ggcggtgaga tctaccacat gagcggtagg cggtgtcaat
     4021 tgcttggatc cgtgtggata aagttccccc tggcccatcg ggcgcaaacc ttcgcaggca
     4081 acgcacaaag acagtgagtc catggatctc ttcgaatacc aagcgaaaga gctgttcgcc
     4141 aagcacaacg tgcccaccac cccgggtcgg gtgaccgaca ccgcagagga agccaaggcc
     4201 atcgccaccg aaatcggtcg gcccgtcatg gtcaaggcgc aggtgaagac cggcggccgc
     4261 ggcaaggccg gcggcgtcaa gtacgcagcc acccccgacg acgcgttcac gcacgcgcag
     4321 aacatcctcg gcctggacat caagggccac atcgtgaaga agctgctggt ctcggaggcc
     4381 agcgacatcg ccgaggagta ctacatctcg ttcctgctcg accgcgctaa ccgcacctac
     4441 ctcgcgatgt gctcggtcga gggcggcatg gagatcgaag aggtcgcagc gaccaagccg
     4501 gagcggttgg ccaaggtgcc ggtcgacgcg gtcaagggag tggacctggc gttcgcccgg
     4561 tcgatcgccg agcaggggca tctgcccgcc gaggtgctcg acgcggcggc cgtgacgatc
     4621 cagaagctgt gggagctctt cgtcgccgaa gacgccacgc tggtcgaggt gaacccgttg
     4681 gtgcgcacgc cggacgacca gatcctggcg ctggacggca aggtcacgct cgacgcgaac
     4741 gccgacttcc gtcagcccgg gcacgccgag ttcgaggacc gcgacgccac cgatccgctg
     4801 gagctgaagg ccaaggcgaa cgacctcaac tacgtcaagc tcgacggtga ggtcggcatc
     4861 atcggcaacg gtgcgggcct ggtgatgtcc acgctcgacg tcgtcgcgta cgcgggcgaa
     4921 aagcacggcg gggtcaagcc cgccaacttc ctcgacatcg gtggtggcgc gtcggccgag
     4981 gtgatggccg ccggtctcga cgtcatcctg cacgacccgc aggtgaagag cgtgttcgtc
     5041 aatgtgttcg gcggcatcac cgcatgcgac gccgtcgcca acggcatcgt gacggcgctg
     5101 aagatcctcg gggacgaggc caacaaaccg ctggtggtcc gcctggacgg caacaacgtc
     5161 gacgagggcc gacgcatcct cgccgaggcc aaccacccgc tggtgacgga ggtggccacg
     5221 atggacgaag ccgccgacgt cgctgcagcc aaggccgccg aagcagcgaa cgcctgaaag
     5281 gactgaaatg gcaatctttt tgaccaagga caacaaggtc atcgtccaag gcatcaccgg
     5341 cgcggaggcc accactcaca ccgcgcggat gctcaaggcg ggcacccaga tcgtcggcgg
     5401 cgtcaacgct cgcaaggccg gaacgacggt gtcgcacaag gacgtcaacg acaacgacat
     5461 cgagctgccg gtgttcggca ccgtcgtcga ggcgatcgag aagaccggcg ccgacgtgtc
     5521 gatcatcttc gtgccgccca aattcgccaa ggacgcgatc atcgaggcga tcgacgcgga
     5581 gatcccgctg ttggtggtca tcaccgaggg aattccggtg caggacagcg catatgcgtg
     5641 ggcctacaac gtcgagaagg gcaagaagac ccgcatcatc ggaccgaact gccccggcat
     5701 catcaccccc ggccaggcgc tggtcggcat cacgccggcc aacatcagcg gcgccgggcc
     5761 gatcgggctg gtgtcgaagt ccggcacgct gacctaccag atgatgttcg agttgcgcga
     5821 tttcggcttc tccacctcca tcggcatcgg tggtgatccg gtcatcggca ccacgcacat
     5881 cgatgccatc gaggcgttcg agaaggatcc cggcaccaaa gtcatcgtga tgatcggcga
     5941 gatcggcggc gacgccgagg aacgtgccgc cgactacatc aaggcgaacg tgtccaagcc
     6001 ggtcgtcggc tacgtcgcgg gattcaccgc gccggaaggc aagacgatgg gccacgccgg
     6061 cgccatcgtc tccggctcgt cgggcaccgc ggccgccaag aaggaggccc tcgaggcggc
     6121 gggcgtcaag gtcggcaaga ccccgtcgga gaccgctcgt ctcgcgcggg agatcctgca
     6181 gagcctctag gcactcggcc cactgccgcg accgtgcgtg tctgtacacc gacacgccgc
     6241 tatttgcgcg cattttgcgc acgctcgcga cccagcgcga tgtgaactag accgcaggat
     6301 taggaacgcg ttctagttat cgattagcct gacgaattaa cacgcgtcag gaggtctgta
     6361 atggtcgatc cgcggacccc ggtgatcgtc ggcgtcggcc agttcactga acgcatccac
     6421 gacgccgact accgagggat gtcggcggtg gaactggcga cacaagccgc gcgcgccgcg
     6481 ctggccgaca ctggcgtcga caacgcaaag atcgcctcgg cgatagacac cgtgttcgcg
     6541 ctgcggcagt tcgagatctc cgggccggcg cccgcgaagc tcggcaggtc caacaactac
     6601 ccgcgctcgg tgatgaatcg gctgggcggc gaccctgccc gcgtcgtgct ggaacccgtc
     6661 ggcgggcaga gcccgcagaa gctcgtcacc gaggcgggta acacgatcgt gtcgggcgac
     6721 gccgacgtcg tgatgatcat gggctccgag ccggggtcca cggcgaggta cttcgcgaac
     6781 cgggatgaca agccggactt caccgagcat gtcgacggac agctcgagga ccgcggccac
     6841 cagatctaca cctacatcga cgactacacg attcagcacg ggctcaccgg tgcgccggtc
     6901 cagtacgggt tgctggagaa cgcccgccgg gctcgcctcg ggctcaatgt gaccgactat
     6961 cggcggagga tggccgaact cttcgccccc ttttcgaaag tggctgccaa gaacccgttt
     7021 tcatcgtcgc cggtagagcg atcggtcgac gagatcctca cggtcaccga cgacaaccgg
     7081 atgatctgtg acccgtatcc ccgcctgctc gtcgcgcgcg atcaggtcaa tcagggtgca
     7141 gcggcggtga tgatgtccgt cgaggcggcc cgtcggctcg gtgtggcgga ggacaagtgg
     7201 gtctacgtcc acggccacgc cgacttggtt gagcaagcga cactggaccg ggccgacctg
     7261 ggcgccggcc cggcgacggt gatggccgcg cgagaagcat tgcgggtggc cggaatcggc
     7321 gtcgacgaca tcgccacctt cgatctgtac agctgcttcc cgtttcccgt tttcctcata
     7381 tccgaggctc tcggtatcga cggcgacgat ccgcgcgggc tgacgctcac cggcggactg
     7441 ccatatttcg gcggccctgg caacagctat tcgctgcacg gtatcgccga gacggtgatc
     7501 gaaatgcgcg aagcgccagg gcaattcggc ttcgttggcg caaacggcgg catcatgagc
     7561 aagtactcgg tcggcatcta ctccaccgag cccgtcgaat ggcgcaccga ccgcagcgag
     7621 gaactgaagc aggagatcgc cgcgctgcca aaggttccgg tgagccggaa tcccgacggc
     7681 gccgcgacaa tcgagaccta ctcggtgcgc tacgactggc cgatccgcac gggcatcatc
     7741 gtcggacgcc tcgatgccga taactcacgt ttcatggcca ccaccgacga cgaggatctg
     7801 gtggcgctga tgtccgacgg cgatccgcta ggcgcggcga tcagcgtgtg gtcgaccgag
     7861 cacggcaacc gcgcttcgct cgcctaaccg gccgcggtgt gcgcgacgat cgcaccgacg
     7921 attgtctccc aatcggctcg atcgactccg tgaccggaac catccagcgg caacagcttt
     7981 gcgcccggaa tcgcgtccgc aagcgcctcg ccatgcgcca cggggaacat cggatctgcg
     8041 ctgccatgga tcaccagtgt cggcacggag atcgacgaca ggggcgcgcg tggcggctcg
     8101 ccgtccggga gcacgtcgtg gttctgtgcc gccgcgaaat catgcgcgcg ctcgacatcg
     8161 cggcgaacga gcacgcgcat cggcccctca tcgaacgggc gcatggctcc cgcgagcaca
     8221 cgcgagtagt cgaccaggta ctcgatcacc gactccgaat cggaccagtc cacctccgcc
     8281 gttgtcacga accgcgtgaa atcctcagtc ggcggcggga gttcacgttc gcccggcagt
     8341 gctgacgagg tactgatcag aacgagcgat cgcacgcggt ccgggaagtc gagtgcaagc
     8401 agctgcgcga tcgctccgcc tgccgaaact ccgaccacat gggcggctgg aacacggtag
     8461 gcgtccatca ctccggccgc gtcggcgact aggtccgcac tcgtgtatcc ggggcgtccc
     8521 ggctcgtagg tgatcgatcg gccggtgtcg cgatggtcgt agcgaatcac gaaccgccca
     8581 cgttcggcga gcatccggca gaagccctcg tcccaccaca acatcgacgc gccggtgccc
     8641 atcaccagaa gaatcggtgg gtcgcccggg tccccgaacg gctcggtgca cagctcgacg
     8701 ccgttcgcct cgatcattcg ctccatcgcg cgcgacgtta gtgcaacgtc gcgtccgaat
     8761 cgcctagacg agcgccgcca gctttccggc cagacgctcc acgtagctcg cgacctcggc
     8821 ctcggctttg tcgggcacgc cgaacagcac ctcggtcacc cccaggtcgg cccaccgggc
     8881 cagcttgtcg gggtccggct tgaagtccag tgcgacgatc tgcggggcac cgtcgcgccc
     8941 ggccgccgcc caagtgtcct gcagcagctt gaccggctcg tcgatgtcga agtcgcgtgg
     9001 cgtggtgatc cagccgtcgg cgctgcgcgc gatccacttg aagttcttct cggtaccggc
     9061 cgcacccacg agcaccggga tgtgcgactg gatcggcttg ggccacgccc agctcggtcc
     9121 gaagttgacg aactcgccct cgtaggacgc ctcctcctgc gtccacaacg cgcgcatggc
     9181 ctcgaggtac tcgcgcagca tcgtgcgtcg ccgggccgga ggcacgttgt ggtcggcgag
     9241 ttcgtctgtg ttccaaccga atcccacgcc aacgctgacg cgtccacccg acaggtgatc
     9301 cagcgtggcg atcgacttgg ccagcgtgat cggatcgtgc tcgaccggca gcgccac
//
LOCUS       DASRJA010000101        15690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_533670,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000101 DASRJA010000000
VERSION     DASRJA010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15690
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533670"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..295)
                     /gene="rpsR"
                     /locus_tag="VFC01_04605"
     CDS             complement(35..295)
                     /gene="rpsR"
                     /locus_tag="VFC01_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003946414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZN78961.1"
                     /translation="MMAGKKSRMRRPAPPELKRQKKNPLKALGVTTIDYKDTHLLRTF
                     ISERGKIRSRRVTGLSPQQQRQVATAIRNAREMALLPFVETK"
     gene            385..1305
                     /locus_tag="VFC01_04610"
     CDS             385..1305
                     /locus_tag="VFC01_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN78962.1"
                     /translation="MRLKLGRPDLSRYAGSFDAPAAGPDAPLTVTWAGVTTLLIDDGT
                     SALMTDGFFSRPGLLEVGLKRLSPSAPRIDGCLSRLGVDRLEAVLPVHTHFDHAMDSA
                     VVAERTGARIVGGTSAVQVGRGGGLPDERLTALTPGEPTACGAYDVTLIEAEHCPPDR
                     FPGVIREPVVPPVKVSAYKCGEAWSTVVHHRPSNRRMLVVGSAGFVRGALVGQRAEVV
                     YLGVGQLGLQHEQYLIDYWTETVRTVGARRVVLIHWDDFFRPLHQPLRALPYAGDDLD
                     VSMRVLSRLAEEDGIPLHLPTLWQRCDPWS"
     gene            1296..2531
                     /locus_tag="VFC01_04615"
     CDS             1296..2531
                     /locus_tag="VFC01_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZN78963.1"
                     /translation="MELTLALVALAVVLGFAMVRPRGWPEAVAAVPTAGLLIAFGVIS
                     WHDAAAEMTRLLPVVGFLAAVLVLARLCDDEGLFRAAGAAMARSNSGNQQRLLAMVFV
                     IAAATTAILSLDATVVLLTPVVLATARALRVPARPHAYATAHLANTASLLMPVSNLTN
                     LLAFSAAGLSFVHFTAVMAVPWLAAVGVEFILLRWLFARELSVEPTHDTLPEDVQVPV
                     FVLVVLALTLVGFAVVSLFGLSPAWVALAGAVVLAVHGLIRGRSTVSGIADALDVPFL
                     AFVLCLGVVVAAVMLNGLDEAMRDVLPSGHTLTALLGIAAVAALLSNVVNNLPAVLVL
                     LPLVSGPAAVLAVLIGVNIGPNLTYVGSLANLLWRSVVRRDIRAGFVEFTRIGLLTTP
                     LTLVVAVLGLWLGIRVFGV"
     gene            complement(2528..3922)
                     /locus_tag="VFC01_04620"
     CDS             complement(2528..3922)
                     /locus_tag="VFC01_04620"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZN78964.1"
                     /translation="MAPLKSDTCVELSFRGRFNFGIDQWEYVPFDVPDGTQRIDVRTS
                     HDQFSLLGIGRNVLDLGIFGPAGHDLGNAEGFRGWSGGARAGFMLSAVNATPGYLAGP
                     IDPGRWALALGPVVLNPLGMDWHAQITLTRGVQPSLRPAPDSHPAGRRSSRPGWYRGD
                     LHTHTVHSDGRRRIAEMAAAATSAGLDFIVSTDHNTSSANRAWAVTEHDSLEVVAGEE
                     VTTRHGHWLAIGLPPGGWIDWRYAPRDGVFATYAARVRADGGLVVAAHPSVPLPGCAW
                     EFGYHDVDAMEVWNGLWNVDDELSLRIWQQLLRQGRRIAAVGGSDSHTSSQPVGRPQT
                     VVYAQGLCTPDLLDGLRRGRSYLTESSAVSLALTAFRGHGAAAGPGETLTVRPDTPLT
                     VAAEVTGAAESSLAIITDGGCVGRVKTDDCGAAKLRWDCMAGEARFARVEVRRRTRFP
                     SMVALSNPVWLQSL"
     gene            4083..4841
                     /locus_tag="VFC01_04625"
     CDS             4083..4841
                     /locus_tag="VFC01_04625"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZN78965.1"
                     /translation="MFSRYVALGDSFTEGVGDPHPTSPNGLRGWADHVAIALAQGNPD
                     LRYANLAVRGRRMDEILADQLQTAVMLEPDLVIVYAGMNDLLLVRNDIDAMMARYADA
                     LKTLQQTGAHVLAFTAGDLGTVPLFRRLRGRAAVYNELLRGIADDLGLQLVDFWRFTE
                     FRDPRMWASDRIHLSPLGHERMAAKVLDTLTIPHQLPTRSAALLSSSPPVRNFWANLQ
                     WTFAFAAPWVAKRLRRATPGAGVEPKYPTLTRIL"
     gene            4856..6292
                     /locus_tag="VFC01_04630"
                     /pseudo
     CDS             4856..6292
                     /locus_tag="VFC01_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(6289..7227)
                     /gene="rlmB"
                     /locus_tag="VFC01_04635"
     CDS             complement(6289..7227)
                     /gene="rlmB"
                     /locus_tag="VFC01_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZN78966.1"
                     /translation="MAGNSRRRGAVRKPGTKKGPTVGSGGVRRRGLEGRGATPPAHMR
                     PKHPAAKRAAKSAKQQRTPRKTDDTEIVLGRNPVLECLRAGVPATALYVALGAEADER
                     LTESVRIAADSGISILEVQRHDLDRIAANGLHQGIALQVPPYQYAHPDDLLGSATKDS
                     VPALMVALDNISDPRNLGAIVRSVAAFGGHGVVIPQRRSASVTAVAWRTSAGAAARLP
                     VARATNLNRTLKNWADAGLQVIGLDADGDTNVDELDGTGPMVVVVGSEGKGLSRLVRE
                     NCDAVVSIPMAGPTESLNASVAAGVVLAEIARQRRG"
     gene            complement(7228..8631)
                     /gene="cysS"
                     /locus_tag="VFC01_04640"
     CDS             complement(7228..8631)
                     /gene="cysS"
                     /locus_tag="VFC01_04640"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZN78967.1"
                     /translation="MTDGMRLYDTMTGAVREFVPLKPGHASIYLCGATVQGLPHIGHV
                     RSGVAFDVLRRWLMAKGYDVAFIRNVTDIDDKILNKAADAGRPWWEWAATYERAFAAA
                     YDALGVLAPSAEPRATGHITQMVELIERLIDRGHAYTGNGDVYFDVLSLSEYGKLSGH
                     KIDDVHQGEGVAKGKRDERDFTLWKGAKPGEPSWPTPWGRGRPGWHTECVAMCEAYLG
                     PEFDIHAGGMDLVFPHHENEIAQAEAAGDAFARFWLHNGWVTMGGEKMSKSLGNVLAI
                     PAVLQRVRPAELRYYLGSAHYRSMLEFSETALQDAAKAYAGIEDFLHRVRNRVGAVVP
                     GEWTPKFAAALDDDLSVPIALAEVHAARAEGNRALDAGDHETALAHAMSIRAMMGILG
                     ADPLDERWESHDETSAALAAVDVLVQAEVQRREDARVRRDWAEADAIRDRLKEAGIEV
                     TDTADGPQWALLDGYTK"
     gene            complement(8662..9144)
                     /gene="ispF"
                     /locus_tag="VFC01_04645"
     CDS             complement(8662..9144)
                     /gene="ispF"
                     /locus_tag="VFC01_04645"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZN78968.1"
                     /translation="MMSIPRVGLGTDVHPIQAGRPCWLLCLLFDDADGCAGHSDGDVA
                     AHALCDALLSAAGLGDIGDVFGTDRPEWRGVSGADMLRHVSGLLTAEGYRIGNAAVQV
                     IGNRPKVGPRRAEAQKVLSELLDAPVSVSATTTDGLGLTGRGEGLAAIATALVLGSGD
                     "
     gene            complement(9150..9638)
                     /gene="carD"
                     /locus_tag="VFC01_04650"
     CDS             complement(9150..9638)
                     /gene="carD"
                     /locus_tag="VFC01_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     CarD"
                     /protein_id="HZN78969.1"
                     /translation="MIFKVGDTVVYPHHGAALIEAIETRTIKGEQKEYLVLKVAQGDL
                     TVRVPAENAEYVGVRDVVGQEGLDKVFQVLRAPHTEEPTNWSRRYKANLEKLASGDVN
                     KVAEVVRDLWRRDQERGLSAGEKRMLAKARQILVGELALAENTDDAKAATILDEVLAA
                     AS"
     gene            9900..10460
                     /locus_tag="VFC01_04655"
     CDS             9900..10460
                     /locus_tag="VFC01_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78970.1"
                     /translation="MNRFKVAACGLAAAIALSGCGAGQVSQTATQEPAVNGTSATLGT
                     IALRNIHLRAVQTTDYVQPGRDVELIFTAVNTSPDVNDKLVSITSDVGTVSLTGDTTV
                     PAGGVLVVGQPDGQVAALESAEAAEAVKAKVDISKPITNGLTYNFTFDFEKSGETTVP
                     VPISAGEAPRRVEEPLTENADTGGHH"
     gene            10494..11873
                     /gene="radA"
                     /locus_tag="VFC01_04660"
     CDS             10494..11873
                     /gene="radA"
                     /locus_tag="VFC01_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HZN78971.1"
                     /translation="MAKARSQYRCSECHHVTAKWVGRCPDCGTWGTVDEVAVLAAVNG
                     SALRRAVAPTSPAVPISSIDPGSTRHFHTGVTELDRVLGGGLVPGSVTLLAGDPGVGK
                     STLLLEVAHRWAQSGRRALYLSGEESAGQIRLRAERTRCTHDEVYLAAESDLQMALGH
                     IDQVKPSLVVVDSVQTMSTTEADGVTGGVTQVRAVTTALTMAAKTSGVAMLLVGHVTK
                     DGAIAGPRSLEHLVDVVLHFEGDRTSALRMVRGVKNRFGAADEVGCFLLHDNGIECVA
                     DPSGLFLDQRPKPVSGTAVTVTLDGKRPLIGEVQALIGSPATGSPRRAVSGIDSSRAA
                     MITAVLEKRARLPVGSNDIYLSTVGGMRLTDPSSDLAVALAIASAYTDLPMPTNAVAL
                     GEVGLAGDLRRVSGMDRRLAEAARLGFGCAVVPDGVTAVPAGLRALPADNIQTALRVL
                     RQISQTDAG"
     gene            11932..13014
                     /gene="disA"
                     /locus_tag="VFC01_04665"
     CDS             11932..13014
                     /gene="disA"
                     /locus_tag="VFC01_04665"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HZN78972.1"
                     /translation="MVQLARPTLRETIARLAPGTALRDGLERILRGRTGALIVIGYDD
                     SVEGICDGGFELDIRYAPTRLRELSKMDGAVVLSSDGGRIVRANVQLVPDPSIPTEES
                     GTRHRSAERTAIQTGYPVISVSHSMSIVTVYVAGERHVIPDSAAILSRANQTIDTLER
                     YKTRLDEVSRQLSTAEIEDFVTLRDVMTVVQRLEMVRRISLEIDSDVVELGTDGRQLK
                     LQLEELAGDIDTARELIVRDYHANPDPPTAAQVSATLEELDSLSDNELLDFTTLARVF
                     GYPSTAEAQDSAMSSRGYRAMAGIPRLQFAHVDLLVRSFGSLQGLLAATSDDLQSVEG
                     IGSMWARHIREGLSQLAESTIADRLA"
     gene            complement(13011..13937)
                     /locus_tag="VFC01_04670"
     CDS             complement(13011..13937)
                     /locus_tag="VFC01_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78973.1"
                     /translation="MTDLVQSIRAAEDSERGEVTPKLEPPAAPVSGETRSFRWQTIGL
                     FACGALGSLAIVWFIVFSRGQLGSKAEWFFGAAVFCVVMASMWQTLNIQRQANQRAAD
                     AVERLRTELVAAEERAAREVAMAQALHRVEMEARERAFRAEVEAQRELARVERGHLIN
                     QLQKQAMVEVSRAVNGHTQMLATLWNEGARILRIEDRDEREQAMNPIFEQISRVVNDF
                     SVEVSNANVLLEDDRLNRALNQVNEAALMAIRVAQDVHDAVVEGRTPKPDSMRTVQRL
                     MQTRAAEARRLAWELLRHGLDDTRAATESAPN"
     gene            14108..14689
                     /locus_tag="VFC01_04675"
     CDS             14108..14689
                     /locus_tag="VFC01_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZN78974.1"
                     /translation="MAQLAPQERDSAIIVDLAEAAMHMYTAAIDALPFAEDRKFHKRA
                     DVVLSGLRKLRAALTDAASTSRPSPAVIVALSNVRRRYDSLMEHAAAAPGSSLGQQIY
                     VTRVHAKLSATEVANGAGVRDDLLDELEAGGTPTDEEATRIKDAIAALGGVPGTEHLQ
                     PQQPEPKPEPEPEESAEPHVNGWDGELVSDNAG"
     gene            14737..15249
                     /locus_tag="VFC01_04680"
                     /pseudo
     CDS             14737..15249
                     /locus_tag="VFC01_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602549.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(15487..>15690)
                     /locus_tag="VFC01_04685"
     CDS             complement(15487..>15690)
                     /locus_tag="VFC01_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZN78975.1"
                     /translation="SSYLFTHPWVNDFFTSLKGKPRDEMRTDVQTYMDANPQVRDELT
                     AVRQPATDFRDRCNAPIPDGPMG"
BASE COUNT         2534 a         5284 c         5157 g         2715 t
ORIGIN      
        1 aggctcgtcg cggacggcgt cgagttgaag ccgtttactt agtttcgacg aacggcaaca
       61 gcgccatttc ccgcgcgttc ctgatcgccg tcgcgacttg acgttgctgc tgaggcgaca
      121 accccgtcac ccggcgcgac cgaatcttgc cgcgctcgga gatgaacgtg cgcagtagat
      181 gggtgtcctt gtagtcgatg gtcgtgacgc cgagagcctt gagcggattc ttcttctgtc
      241 gcttgagctc tggcggcgcg ggacgccgca tccgtgactt cttgcctgcc atcaggtact
      301 ccagttgcgc atgaatgcac cgatcaggtg tgccggaagt ctaccgacgc aaaagagtcc
      361 ccgtgcgcga ttaaggttgg cgatatgcgc ctcaagctcg gacgccccga cctgagccgc
      421 tacgcgggat ccttcgacgc accggcggcc ggtcccgacg caccgctgac ggtgacgtgg
      481 gcaggcgtca ccaccctgct gattgacgac ggcacctccg cgctgatgac cgacggcttc
      541 ttctcgcgac cgggcctgct cgaggtgggg ttgaagcgct tgtcgccgtc ggcgccgcgg
      601 atcgacggct gcctgagccg cctcggcgtg gaccggctcg aagccgtgct gcccgtgcac
      661 acgcatttcg accacgcgat ggattcggcg gtggtcgccg aacggaccgg cgcacggatc
      721 gtcggcggaa cgtccgcggt acaggtcggc cgcggcggtg ggctacccga cgagcgcctc
      781 accgctctca cgccgggcga gccgacagcg tgtggcgcat acgacgtcac gctgatcgag
      841 gccgagcact gcccacccga ccgctttccg ggtgttatcc gcgaaccggt ggtcccgccg
      901 gtgaaggttt ccgcatacaa gtgcggcgag gcctggtcga cggtggtgca ccaccggccg
      961 tcgaatcgtc ggatgctggt cgtcggcagc gcaggctttg tccgcggcgc gctcgtcggg
     1021 cagcgggccg aggtcgtgta tctgggtgtg ggacaactcg ggctacaaca cgagcagtac
     1081 ctcatcgatt actggaccga gaccgtccgc accgtcggcg cgcgccgggt cgtgctcatt
     1141 cactgggacg acttcttccg cccgctgcac cagccgctgc gggcgctgcc gtacgcgggt
     1201 gacgatctcg acgtgtcgat gcgggtgttg tcccggctgg ccgaggagga cggcatcccg
     1261 ctgcatctgc ccacgttgtg gcagcgctgc gacccgtgga gttgacgctg gcgctggtcg
     1321 cgctggccgt ggtgctcgga ttcgcgatgg tccgcccgcg cggctggccc gaggccgtcg
     1381 ccgcggttcc caccgcaggc ctgctgattg cgttcggtgt gatctcctgg cacgacgctg
     1441 cggccgaaat gacccgactg ctaccggtcg tcgggttcct cgcagcggtg ctcgtgctgg
     1501 cccggttgtg tgacgacgag gggctgttcc gcgctgccgg ggcggccatg gcccggtcga
     1561 actcgggcaa ccagcagcgg ttgctggcaa tggttttcgt gatcgcggcg gccacaaccg
     1621 cgatactgag cctggacgcg accgtcgtgc tgctgacacc ggtggtgctc gcgaccgccc
     1681 gagcgttgcg ggtgcctgca cgtccgcatg cctacgccac cgcacatctg gccaacacag
     1741 cctcgctgct gatgcctgtg tcaaatctaa ccaacctgct ggcgttcagc gcggcggggc
     1801 tgtcgttcgt ccacttcacc gccgtgatgg cggtgccctg gcttgccgct gtcggcgtgg
     1861 aattcatttt gctgcgttgg cttttcgcac gggagctgtc cgtagagccg acgcatgaca
     1921 ccctgcctga ggatgtgcaa gtgccggtgt tcgtgctcgt cgtgttggcg ttgacgctcg
     1981 tcgggtttgc cgtcgtttcg ttgttcggct tgtcgcctgc gtgggtggcg ttagcgggtg
     2041 cggtggtgct cgccgtgcac ggcctgatcc gtggccgcag cacggtctcc gggatcgcgg
     2101 acgcgctgga cgtgccgttc ctggcgttcg tgttgtgcct cggtgtggtc gtcgccgcgg
     2161 tcatgctcaa cggcctcgac gaagccatgc gcgacgtgtt gccgagcggc catacgttga
     2221 ccgcgctgct ggggatcgcc gcggttgcgg cgctgctgtc caacgtcgtc aacaacctgc
     2281 ctgcggtgct ggtgctactg ccgttggtgt ccggaccggc cgctgtgctc gcggtgctca
     2341 tcggagtcaa catcgggccg aacctgacct acgtcggctc cctcgcgaac ctgctgtggc
     2401 gcagtgtcgt gcgccgcgac atcagggcag ggttcgtcga gttcacccgc atcggattgc
     2461 tcacgacgcc gctgacactc gtcgtcgcgg tgctcggcct atggctcggg attcgggtct
     2521 tcggcgtcta aagcgactgc agccagaccg gattggacag cgccaccatc gatggaaaac
     2581 gtgtccggcg gcgcacctcg actcgggcga accgagcctc gccggccatg cagtcccacc
     2641 gcaacttcgc cgctccgcag tcgtcggtct tcacgcggcc gacgcagccg ccgtcggtga
     2701 tgatggcgag gctgctttcc gccgcgcccg tcacctccgc ggcaacggtc aggggcgtgt
     2761 cggggcgcac tgttagcgtc tcacccggtc ccgccgctgc gccgtggccg cggaatgcag
     2821 tgagcgccaa ggacaccgcg ctcgattcgg tcagatagct gcgaccgcga cggaggccgt
     2881 cgaggaggtc aggcgtgcac aggccctgcg cgtacaccac ggtctgcggc cgcccgaccg
     2941 gttgactcga agtgtgtgaa tcgctgccgc ccaccgcggc tatccgcctg ccctgccgca
     3001 gcagctgctg ccagatgcgc agcgacagtt cgtcatcgac gttccacagc ccgttccaga
     3061 cctccatggc gtccacgtcg tgatagccga actcccacgc gcatccaggc agcggcacgg
     3121 atggatgcgc ggccaccacc aagccgccgt ccgcccggac ccgcgcggcg tacgtcgcga
     3181 agacaccgtc gcgcggtgcg taccgccagt cgatccagcc gccaggaggc agaccgatcg
     3241 ccagccagtg accgtggcgg gtggtgacct cctcgcccgc aacgacctcc aggctgtcgt
     3301 gttcggtgac cgcccacgcc cgattggcgg agctggtgtt gtgatcggtg gagacgatga
     3361 agtccaggcc ggctgatgtg gcggccgcag ccatctcggc gattctccgc cgaccatcgg
     3421 aatgcactgt gtgcgtgtgt aagtcaccgc gataccaacc cggtcgactg ctgcggcggc
     3481 cagcggggtg ggagtcggga gcgggacgca acgacggctg cacgccccgg gtcagcgtga
     3541 tctgcgcgtg ccagtccatg cccagcggat tgagcaccac cggcccgagc gccaacgccc
     3601 agcggccggg atcgatcggg cccgccaggt agccgggtgt cgcgttgacc gcggacagca
     3661 tgaaccccgc acgcgccccg cccgaccaac cgcggaagcc ctccgcgttg ccgaggtcat
     3721 ggccggccgg cccgaagatg cccagatcga gcacattgcg gccgataccg agcagcgaga
     3781 actggtcatg cgacgtcctg acgtcgatcc gctgggttcc gtcgggcacg tcgaacggca
     3841 cgtactccca ttggtcgatg ccgaagttga accggccacg aaacgagagc tcgacgcagg
     3901 tgtccgattt cagcggcgcc atgtcgtctc ctcgtgcggt tcggccacat catcgacggc
     3961 gtaggcctaa gcgacggtta catcgacgcg aacaatggtg ggacggctga cgtcgattcg
     4021 cccaccgacg gcgctgcagg cgcgcaattc gctttactat cggcaagatt ggtctcgtgg
     4081 ccatgtttag caggtacgtg gcgctcggcg actcattcac cgagggtgtc ggcgacccac
     4141 atcccaccag ccccaacggt ttgcgcggat gggcagatca cgtcgcaatc gcgctggcgc
     4201 aaggcaaccc cgaccttcgc tacgccaacc tcgcggtacg cggccgcagg atggacgaga
     4261 tcctcgcgga tcaacttcag accgccgtca tgctcgaacc cgacctcgtc atcgtctacg
     4321 caggcatgaa tgacctgctg ttggtgcgca acgacatcga cgcgatgatg gccaggtatg
     4381 cagacgccct gaagacgttg cagcagaccg gggcccacgt gttggcgttc acagcaggcg
     4441 atctcggcac ggtgccgttg tttcggcgac tgcgcggccg agccgcggtc tacaacgagt
     4501 tgttgcgcgg gatcgccgac gacctcggcc tgcagctcgt cgacttttgg cgcttcacag
     4561 agtttcgcga cccgcgtatg tgggcttcag atcgcatcca cctctcgccg ctcggccatg
     4621 agcggatggc agcgaaagtt cttgacacac tgactattcc gcaccagctg cccacacggt
     4681 cggccgctct actgtcctcg tcgccgccgg tacgcaactt ttgggccaat ctgcaatgga
     4741 ctttcgcgtt cgctgcgccg tgggtggcca aacggctacg ccgggcgacg cctggggccg
     4801 gcgtcgagcc gaagtacccg acattgacca ggatcctttg accaccgcca tggatgtgtg
     4861 gcaccacggc gaccgcagcg gcgcaacgcg agtcagcggc cttcatctgc agttcggtgc
     4921 cgacgcggca tccgaagtgg tggtgtcctg gcacacggca gcagcggtga agaacccccg
     4981 cgtcgtgatg ggcacgcccg acgacggcat gggccgtacc gtcgccgcag agacggtgac
     5041 ttatcgcgac gccgcctcgc gcacagaggt ccgcgtgcac catgccaggc tcacgaatct
     5101 tgcacccgac accgactacg tctacgccgc gatgcacgac ggcgccaacc ccgagttggg
     5161 caccgtgcgg acagcgccgc gcggccgcgc cccgctgcgt ttcaccagct tcggcgacca
     5221 atccaccccg acgctggacg cgatggtcgc cagcgcgttc ggccgcgaca accgcggttc
     5281 gccttcggcg ggtgacatca cgaccgcggt cgagcgcgca gacccgttgt tcaacttggt
     5341 caacggcgat ttgtgctacg ccaacctgtc ccaagaccgc gtcaggacgt gggcggactg
     5401 gttcgagaac aactccaggt cagcccgctt ccggccatgg atgcccgctg cgggaaatca
     5461 cgagaacgag ttgggcaacg gcccgatcgg ctacggcgcg tatcaggcct actttgcgct
     5521 tccggattct gattcggatc aacagttgcg agggttgtgg tacgcgttca ccgcgggatc
     5581 ggtccgggtg atcagcctgg cgaacgacga cgtgtgctat caggatggcg gcaactcgta
     5641 cgtgcgtggc tactccggtg gtgcccaaaa gcgttggctc gagcaggaac tcgaacgtac
     5701 ccgcagcgac aacggaatcg actggatcgt ggtgtgcatg caccagaccg ccgtctcgac
     5761 gggtgagcgc accaacggtg cggacctcgg gatccgggag aactggctgc cgctgttcga
     5821 ccgctttggt gtcgacctcg tggtctgcgg acatgagcat cactacgaac gcagtcacgc
     5881 gattcggggc accctgccga ccgacacact gacgccgaac ccggttgaca cacaacccga
     5941 tcacatcgac accagcaagg gtacggttca cctggtcatc ggcggcggag gcacgtcgat
     6001 cccgtcgaac cggatgttct tcgccgagcc acgctgccag gtgctgatgt cggtcgggga
     6061 ggtcaatccg gccactcgtc acaagactcc gcattacgtc caggagaccg caccatggtc
     6121 ggtgtttcgg gaccatgaga atccgtgcgg cttcgtgtca ttcgatgtcg acccgggcca
     6181 acccggcggc aacacgtcga tggctgccac gtactacgcg gtcagcggtc cgttcgggga
     6241 ggtgaccccg gtcgacaagt tcatgttgac gcggcctcgc agagacagtt agccccgtcg
     6301 ttggcgggcg atttcggcca gtaccacgcc ggcggccacg gatgcgttca acgattccgt
     6361 cggcccagcc atcggaatgg acaccacggc gtcgcaattc tcgcgcacca gtcgcgacag
     6421 gcccttgccc tcggagccga ccaccaccac catcggcccg gtaccgtcca gctcgtcgac
     6481 gttggtgtcg ccgtccgcat cgagcccaat cacctgcagt ccggcgtcgg cccaattctt
     6541 cagtgtgcga ttgagattcg ttgccctggc cacgggaagt ctggccgccg cgcccgcact
     6601 ggtgcgccat gccaccgcag tgacggacgc tgaacgccgc tgcgggatca cgactccgtg
     6661 gccaccgaag gccgccacgg atcggacgat ggcgccgaga ttgcgtggat cggagatgtt
     6721 gtccagcgcc accatcaagg cggggacgct gtccttcgtc gcagacccca gcagatcatc
     6781 gggatgcgcg tattgatacg gcggcacctg gagcgcgatg ccctgatgca acccgttggc
     6841 ggcgatgcgg tccagatcgt ggcgctgcac ttccaggatc gagatgcccg aatcggctgc
     6901 gatccttacg gattcggtca gtcgctcgtc agcttcggcc cctagggcca catacaacgc
     6961 ggtcgcagga acaccggcgc gcaggcattc gagcaccgga ttgcggccca gcacgatctc
     7021 cgtgtcatcg gttttgcgcg gcgtccgttg ctgcttagcg gacttggccg cgcgcttggc
     7081 cgccggatgt ttgggccgca tgtgcgcggg aggcgtggca ccacggccct ccagcccgcg
     7141 ccggcgaacc ccgccggacc cgaccgtcgg ccccttcttg gtgcccggct tgcgtaccgc
     7201 accccggcgc cgtgagtttc cagccattta tttcgtgtac ccgtcgagca gtgcccactg
     7261 cggtccgtcg gcggtatcgg tgacctcgat gccggcttcc ttcagccggt cgcggatggc
     7321 gtcggcctcg gcccagtccc gccgcaccct ggcatcctcg cggcgctgca cctcggcctg
     7381 caccagcacg tcgaccgcgg caagcgcggc ggacgtttcg tcgtgggatt cccatcgctc
     7441 gtcaagcgga tcggcgccga ggatgcccat catcgcgcgg atcgacatcg catgagccag
     7501 cgccgtttcg tggtcgccgg cgtccaacgc gcggtttccc tccgcacgcg cggcgtgtac
     7561 ctcggccagt gcgatcggca ccgacaggtc gtcgtccaac gcggccgcga acttcggcgt
     7621 ccactcgccc ggcaccaccg cgccgacccg gttgcggacc cggtgcagga agtcctcgat
     7681 tccggcgtac gccttggccg catcctgcag tgcggtctcg gagaactcga gcatcgagcg
     7741 atagtgcgcg ctgcccaggt agtagcgcaa ctcggctggc cgaacccgtt gcagcacagc
     7801 gggaatcgca agcacgttcc ccaaagactt gctcatcttc tcgccaccca tggtgaccca
     7861 gccgttgtgc agccagaagc gtgcgaacgc gtcacccgcg gcctcggctt gggcgatctc
     7921 gttctcgtgg tgcgggaaca ccagatccat gccgccggcg tggatatcga actcgggtcc
     7981 gagatacgcc tcgcacatcg cgacgcattc ggtatgccag ccggggcggc cgcgtcccca
     8041 cggcgttggc cacgacggct caccgggctt ggcgcccttc cacagcgtga agtcgcgctc
     8101 gtcgcgcttg cccttggcga caccctcgcc ctggtgcacg tcgtcgatct tgtggccgga
     8161 cagcttgccg tactcggaca ggctcagcac gtcgaaatag acgtccccgt tgccggtgta
     8221 ggcgtgtcct cgatcgatca gccgctcgat cagctcgacc atctgcgtga tgtgcccggt
     8281 ggcccggggc tcagccgacg gcgccagcac gcccaaagcg tcgtaggcgg ccgcaaatgc
     8341 ccgctcgtag gtggcggccc attcccacca tggccggccg gcatcggcgg ccttgttgag
     8401 gatcttgtcg tcgatatcgg tgacgttgcg gatgaaggcg acgtcgtagc ccttggccat
     8461 cagccaacgc cgcaacacgt cgaacgccac accgctgcgg acgtggccga tgtgcggcag
     8521 gccctgcacg gtggcaccgc acagatagat cgacgcgtgg ccgggcttca gcggcacaaa
     8581 ttcgcgcacg gcacccgtca tggtgtcgta gaggcgcatg ccgtcggtca cgacgcgcca
     8641 gcttaccggc cttttcgagc cctaatcacc actaccgagc accagggcgg tggcgatggc
     8701 agccaggccc tcccctcggc ccgtgaggcc gaggccgtcc gtggtcgttg cggataccga
     8761 aacgggcgcg tccagcagtt cggaaagaac tttttgcgct tcggcgcgtc gcgggccaac
     8821 ctttggccga ttgccgatca cctgcacagc ggcgttgccg atgcggtagc cctcggccgt
     8881 cagcagccca ctgacgtgcc gaagcatgtc agcgccgctg acgccacgcc attccgggcg
     8941 gtcagtgccg aaaacatcgc cgatgtcccc cagcccagcg gccgacagca acgcatcgca
     9001 cagcgcatga gcggccacgt caccgtcgga atggcccgcg cagccatcag cgtcatcgaa
     9061 cagcaggcac agcagccaac acggcctacc ggcctggatt gggtggacgt cggtgccgag
     9121 cccgactctc gggatcgaca tcatggcctt caggaagcgg cggccagaac ctcgtcgagg
     9181 atggttgccg ccttggcgtc gtccgtgttc tcggcaagcg cgagctcacc gaccaggatc
     9241 tgtcgggcct tggccagcat ccgcttctcg ccggcggaca ggccacgctc ctggtcacgg
     9301 cgccagaggt cacggaccac ctcggcgacc ttgttgacgt cgccggaggc cagcttctcg
     9361 agattggcct tgtaccggcg cgaccagttg gtcggctctt cggtatgcgg ggcgcgaagc
     9421 acctggaaaa ccttgtccag accctcctgc ccgacaacgt cgcgcacgcc gacatactcg
     9481 gcattttcgg cgggtactcg aacggtgagg tccccctggg cgaccttcaa gacgaggtat
     9541 tccttttgtt cgcctttaat ggtccgggtt tcgatcgcct cgatcaacgc agcaccgtgg
     9601 tgtggataga caacggtgtc tccgacctta aaaatcatca gatacgagcc cctttcacga
     9661 ctcaatgtta gcacggggcc tagacaggtg cggatcaacg gtgcaggtca ggggcacagt
     9721 cggtgaagag caggggttga cagggtgaca aatacgtgca ttgcgcgggt ttctttgcaa
     9781 gcaccgacga ggcagggcaa accctcgtct accgcctccg accgctacct actactgtgc
     9841 atagtcgaat ctcagcgacc ggcccgacgg tcgcctcgtc ggtcgagcag gaggcatctg
     9901 tgaaccgctt caaagtcgcc gcgtgcgggc tggccgccgc gatcgcgctc agcggctgcg
     9961 gagccgggca ggtttcccag acggccaccc aagagcccgc ggtgaacggc accagcgcca
    10021 cactgggcac catcgccctc cgcaacatcc acctgcgggc cgtccagacg acggactatg
    10081 tgcagccggg acgcgacgtc gagctgatct tcaccgccgt gaacacctct ccggacgtca
    10141 acgacaagct ggtctcgatc acctccgacg tcggcaccgt atcgctgacc ggcgacacga
    10201 cggtgccggc tggcggcgtc ctggtggtcg gccagcccga cggccaggtc gcggcgctgg
    10261 agtctgcgga agcggccgag gccgtcaagg cgaaggtcga catatccaag ccgatcacca
    10321 acgggctgac gtacaacttc acgttcgact tcgagaagtc cggcgagacg acggtgcccg
    10381 tgccgatctc ggccggagaa gcgcctcgcc gggtcgaaga gccgctgaca gagaacgccg
    10441 acacgggcgg tcaccactaa ggttctgtcg gtcgcaccgg ttacggtcac ggcgtggcta
    10501 aagcgcgttc gcagtaccgc tgctcggaat gccaccacgt caccgccaaa tgggttggcc
    10561 gctgcccgga ttgcggcacc tggggcaccg tcgacgaagt cgccgtactg gccgcggtca
    10621 acggctcagc cttgcggcgc gccgtcgcgc cgacctcccc cgctgtgccg atcagctcga
    10681 tcgacccggg cagcacccgt catttccaca ccggcgtcac cgaactcgac cgcgtcctcg
    10741 gcgggggtct ggttcccggt tcggtgacac tgctcgcggg cgacccgggc gtcggcaagt
    10801 cgacgctgct gctcgaggtc gcccaccgct gggcgcagtc gggcaggcgc gcgctgtatc
    10861 tgtccggtga ggaatcggcg ggccagatcc ggctgcgggc cgaacgcact cgctgcacac
    10921 acgacgaggt gtacctggcc gccgaatcgg atctgcagat ggcgctcggc cacatcgacc
    10981 aagtcaagcc gagccttgtc gtggtcgact cggtgcagac catgtccacc accgaggccg
    11041 acggcgtcac cggcggggtg acccaggtgc gcgcggtgac gacggcgctg accatggccg
    11101 ccaagacatc tggggtagcg atgctcttgg tcggccacgt cacgaaggac ggcgcgatcg
    11161 cggggccgcg ctcgctggag catctcgtcg acgtcgtgct gcacttcgaa ggcgatcgca
    11221 cgtcggccct tcgcatggtg cgcggggtga agaaccgttt cggcgccgcc gatgaagtgg
    11281 gctgtttcct gttgcacgac aacgggattg agtgtgtggc agacccatcg ggactcttcc
    11341 tcgaccagcg ccccaagccc gtatcgggca cagcggtgac ggtcacgctc gacggcaagc
    11401 ggccgttgat cggcgaggtg caggcactga tcggctcccc tgccaccggt tcgccacgcc
    11461 gcgcggtcag cggcatcgac tcgtcccgcg cggcgatgat caccgctgtc ctggagaagc
    11521 gcgccaggct tccggtcggc agcaacgaca tctacctgtc cactgtcggc ggtatgcggc
    11581 tgaccgaccc gtcgtcggat ttggcggtgg cactcgcgat cgcgtccgcg tacaccgacc
    11641 tgcccatgcc caccaacgcg gtcgcgctcg gcgaggtcgg gctggccggc gatctgcgcc
    11701 gcgtcagtgg catggaccgc aggctggcgg aggcggcacg gcttggcttc ggctgcgccg
    11761 tggtgccgga cggtgtcacg gcggtgcccg cgggactgcg cgcccttccg gccgacaaca
    11821 ttcagactgc gttgcgggtg ctgcggcaaa tcagtcagac tgacgccggg tagcggccga
    11881 tcctaggagg gggcgatggc cgtcaaggcg agtggcaggt cgagccgcaa cgtggtgcag
    11941 ctggcccggc cgaccttgcg ggagaccatc gcgcggttgg cacctgggac cgcgctgcgc
    12001 gacgggctgg agcgcatcct gcgcggccgc accggcgcgc tgatcgtgat cgggtacgac
    12061 gacagtgtcg agggcatctg tgacggtggc ttcgagctcg atatccgcta cgcgcccacc
    12121 cggctgcggg agctgtccaa gatggacggc gcggtcgtgc tgtccagcga cggcggccgc
    12181 atcgtgcggg ccaacgtgca gctggtgccc gacccgtcga tccccaccga ggaatcgggt
    12241 acccggcacc ggtctgccga gcgcaccgcg atccagaccg gctatccggt gatctcggtg
    12301 agccactcga tgagcatcgt cacggtctac gtcgcgggcg agcgccacgt cattccggat
    12361 tcggcggcca tcctgtcgcg ggccaaccag accatcgaca cattggagcg ttacaagacc
    12421 cgtctcgatg aggtcagcag gcagctctcg accgccgaga tcgaggactt cgtgacgctg
    12481 cgcgacgtga tgaccgtggt gcagcggctc gagatggtgc gccggatcag tctcgagatc
    12541 gactccgacg tcgtcgaatt gggcaccgac ggccgccagc tcaagctgca gctggaggag
    12601 ctggccggcg acatcgacac cgcccgtgag ctgatcgtgc gcgactacca cgccaacccc
    12661 gacccgccga cggcggctca ggtcagcgcc acgctcgagg agctcgactc gctgtccgac
    12721 aacgagcttc tcgatttcac aacgctggca agggttttcg gttatccgtc gaccgccgaa
    12781 gcgcaggatt cggcgatgag ctcgcgcggc taccgcgcga tggcaggcat cccgcggttg
    12841 cagttcgcgc acgtcgacct gctggtgcga tccttcggat cgttgcaggg cctgctggca
    12901 gccacgtccg acgatctgca gtcggtggaa ggcatcggct cgatgtgggc ccgccacatc
    12961 cgggaagggc tgtcgcagtt ggccgagtcg acgatcgccg accggctggc ctagttcggc
    13021 gccgattccg ttgcggccct tgtgtcgtcg agcccgtgtc ggagcaactc ccacgccagt
    13081 cgacgagctt ctgccgctct cgtttgcatt agccgctgca cggtccgcat cgaatccggc
    13141 ttcggcgtgc gcccttcgac gacggcgtcg tgcacatcct gagccacgcg gatcgccatc
    13201 aacgcggcct cgttgacctg gttcagcgcg cgattgaggc gatcgtcctc aaggagcacg
    13261 ttggcgttgc tgacctctac cgagaagtcg ttcacgactc ggctgatctg ctcgaagatc
    13321 gggttcatcg cctgctcgcg ctcgtcgcgg tcctcgatgc gcaggatgcg ggcgccctcg
    13381 ttccacaggg tggcaagcat ttgagtgtgt ccgttgacgg cacgcgacac ctcgaccatc
    13441 gcctgcttct ggagctgatt gatcaggtgg ccacgctcga cgcgggccag ctcccgttgc
    13501 gcctccacct cggcacggaa cgccctctcc cgcgcttcca tctccacgcg gtgcaatgcc
    13561 tgggccattg ccacttcgcg tgccgctcgc tcctcggcgg ccaccagctc cgtacgtagt
    13621 cgttcgacgg cgtctgcggc gcgctggttg gcctgacgct gaatgttgag ggtctgccac
    13681 atgctcgcca tcacgacgca gaacacggcg gcgccgaaga accactccgc cttggaaccg
    13741 agctggccgc gactgaagac gatgaaccag acgatggcca gactgcccaa ggcaccgcag
    13801 gcaaacaacc cgatcgtctg ccagcggaac gaacgggttt cgccgctaac tggcgcggca
    13861 ggtggctcta gcttgggggt cacttcgccg cgttccgaat cctcggcggc cctaatggat
    13921 tgcacgagat cggtcatcct ggcctccctt cacccgagcg tgccgccatg ccggttcaga
    13981 ctagagcggc atgggttgag gcgtcgcgcc agcacctccc tcgcggtgta cgcgccacgg
    14041 cgcggaccga tagggtcgac cccgtattca ccgctatcga tttgatccgt tggagaattg
    14101 agggctcatg gcacaactcg cgccacagga acgcgattcg gcgatcatcg tcgacctcgc
    14161 cgaagcggcg atgcacatgt acacggccgc gatcgacgcg ttgcctttcg cagaggaccg
    14221 caagttccac aaacgcgcgg acgtcgtgct gtccggcttg cggaagttgc gcgccgcgct
    14281 gactgacgca gcaagcacca gccgaccctc acccgcggtg attgtggcgc tcagcaatgt
    14341 ccgtcggcgc tacgactctc tgatggaaca tgccgccgct gcgccgggat caagtttggg
    14401 ccagcagatc tacgtcacgc gcgtgcacgc caaactgtcg gccaccgaag tggccaacgg
    14461 cgcgggagtt cgcgacgatc tcctcgatga gctcgaggcg ggcgggacgc ccaccgacga
    14521 ggaagcgacg aggatcaagg acgccatcgc cgccctgggc ggcgttcccg gcaccgaaca
    14581 cctgcagcct cagcagcctg aaccgaagcc cgaaccggag ccggaggagt cggcggaacc
    14641 tcacgtcaac ggctgggacg gggaactcgt ctcggacaac gccggctaag cggggtgtgg
    14701 gccagctggc gtgcaggtag agatcttggg cgatgaagcg agatcgagac gatcagggtt
    14761 ggcccggctt ccgaccccac gtcacaggtc ggtccgctag tgagcgctcg cgcggggggg
    14821 tgtttgcgct catttcccaa gcgactgcgg agggtgcggt gatcgcgcac caggccaacg
    14881 ccccgaccga cggttggtac gttgcgccca cactgctcac cggcgtgcgc cccgacgccg
    14941 ccatcctgcg gcaggagatt ttggccccgt tgctccaatt gtgttgtggg acaacgaaga
    15001 ggagctgccc tgttggatca acgacacgga gtacagtctt gccgcgtatg tctacaccag
    15061 ccgcctacaa gaggccctgc gcatcggcga atctattaac gccggcatgg ttgggatcaa
    15121 ccgcggcata gtgtccgacc cgtcggcccc cttcggcggg acgaagcaaa gcgggctcgg
    15181 acgcgaaggc ggccggaggg gatgcgtgag ttccaggaga cccagcactt caacgtggaa
    15241 ttcgaatgac caggcaggga tcgtggccgg cccgcgggtg ccctgaacgc atagtcacca
    15301 aatgctcact gcccaaaggt tttcgcggaa cgatcctgga ggttagtgtc gcccgacgac
    15361 ggtcacggtg tccagccctg ccccgtcggg ttcgcggatc agctcgagct gcggtgccgc
    15421 cttgatcagc tcggccgtct gtatcgggtg gctcgtgcca gctcggggta gcgatacaac
    15481 agcgcgctag cccatgggcc cgtcggggat aggcgcgttg cagcggtcac ggaagtcggt
    15541 ggcgggctgc ctgacggctg tcagctcgtc tcgcacctgt gggtttgcgt ccatgtacgt
    15601 ctggacatcc gtgcgcatct cgtccctggg cttgcctttg aggctggtga agaagtcgtt
    15661 cacccaagga tgggtgaaca ggtaggacga
//
LOCUS       DASRJA010000102        13768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_483563,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000102 DASRJA010000000
VERSION     DASRJA010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13768
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483563"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4284)
                     /locus_tag="VFC01_04690"
     CDS             complement(<1..4284)
                     /locus_tag="VFC01_04690"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZN78976.1"
                     /translation="MGYARYVGRVGGLAVALGIGVAVATTPGVALAGPSDPDSSNSPS
                     PGSPSAPSGSAAGSPSSSPGTDTQSPSSSDSPKTPDASGDDEASGDDSDGAAGDEESK
                     GSDTSETDKKSETVKDKKSGSDASTAAVTPKKPKRAVAHTPSDSDVRKGEPAADSGPA
                     VQPKSEPMAPPTVGSVSVDVGLQSASSSASTVQDLPKVEPAPEPDVRPLSSTVLSAVG
                     LAPSADDDAPEVPGESPLLLAGLAAFRRQTQQSLVGDEESTLKVAEPSGSLLMAAVVA
                     NSAPSVSPVVGVPDQSSGVVSVSLNAVDGEGDSLSYAVTGQPVSGTVSVSGAGVVTYT
                     PTAVARLAAGSTSVPDFDSFTVSVSDGETSTPVSVSVPVLPAVWTNQSSSSNVTGVSP
                     YGVAVVGTTAYVANQGANTVSVINTVTGQAVGSPIVVGSAPTGVVASLDGAYVFVSNR
                     NSGTVSVIRTSDNKVIDINPATPTTVDAIKVGSQPEMLAINTSTITTATGEIAAGARL
                     YVANYASNTVSVIDISNPVAPKVIDTNPATTTTVDAITVGSNPRGIAFAETVNGPRVY
                     VVNRTGGTVSVIDAVTNKVVDANPATPTTVDAIKVGTTPQLIVVSPDGTRAYVTNYGS
                     NSVSIIDTATNKVVDANPATPSTVDAIAVPSTPVGVGLSKDGSLLYVANGTDRISVID
                     TKTRAVINTIQIDTAPETNYHTLAVRADGALVVTDLADRALRVVAYQRGNTAPVAIAN
                     PTVGEPDSSTGAVTGLVNIKDWDGDPLTYTTVVAPTKGSLTYDPATGSYTYTPTAAAR
                     DAAANGGPTTDTFTIRATDTYNTSTTTANVTVPILSVGNHAPTAPEFQYFYATDPVTG
                     EVRGRVTVSDVDGDQLSYQLLWGPYGATTFSLDSSTGVFTYIPSREMRESATLYPGYA
                     DYDTFRVTISDGTASVSPWVNLQVMPIQMAPVAYGPPEVPSADPATGRVSGSMNVYDP
                     NGDRITFSMAGLPTRGTATVDSVTGVYTYTPFASQRSAGGLDTFVVSATDGQGSATFT
                     VTVPVRPPELASTQTQIPLSASGPTIAVNGSRAYVVNEYYGTVSAIDTNTNTVIATSS
                     RLVPSSEFYPANLAVSPDGTRLYVANWLQGKVVVVDPNTLTPVGEPIPVQGGADDMVL
                     SPDGKRLYVAHDGWVQSMSVIDTDSRTVIGSIPISSDTRGMVMSPDGRTIYVADGYNN
                     RVPVIDTNTKAVVGSIVLGTATIAPSPGGIALSPDGKWAYVTNPMDNTVAVIDTTSRT
                     VVGTPIVLGVPLASGSQTDWPTGIAVSRDGGSVYVASGDDIMVIDAVTRAVIGAVRFP
                     GYMSDTSASASQTIAVDSSGDILSYGGSGLVSVSLGSMAPGPSQSRMMLADSGAGEPM
                     MLMAAAVVNSAPSVSPVVGVPDQSSGVVSVSLNAVDGEGDSLSYAVTGQP"
     gene            4438..5643
                     /locus_tag="VFC01_04695"
     CDS             4438..5643
                     /locus_tag="VFC01_04695"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN78977.1"
                     /translation="MKLDTNQASIREAIDAGLLAGAVTLVWRSGEVLQVNELGHRDVD
                     ANLDMQRDTIFRIASMTKPVTVAAAMTLVDEGKLALTDPVAKWLPELSDLRVLDAPGG
                     PLDKTHPARRLITVEDLMTHRSGLAYFFSVTGPLAKAYSRISARQNPDRWLAEVAALP
                     LQHQPGDRMTYSNATDVLGILLERSEGKSLQDVLTERILGPLNMADTGFFVASRNRGR
                     AATMYKLEDDDTLSHDAMGPPPIAAPPFCMGGANLFSTADDYLTFTRMLLGGGEVDGV
                     RVLSEKSVRLMRTDRLTDEQKRHPFLGMPFWIGRGFGLNLSVVTDPAKSRQLFGPGGL
                     GTFSWPGAYGTWWQADPSANLILIYLIQNLPNFGADAAAAVAGNTSLLKLQSTQPKFV
                     RRTYQALDI"
     gene            5661..6860
                     /locus_tag="VFC01_04700"
     CDS             5661..6860
                     /locus_tag="VFC01_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZN78978.1"
                     /translation="MAAPTVLISGAGIAGPALAFWLNKSGYRVIIVELADGIRPGGQT
                     VDLRGAGGDVVEHMGLIEQMRQRALDQRGVAWIKSDGRRRAEMPVTAFDGNGLVSKLE
                     ILRGDLVDVLYQATKDQTDYRFDTRIEDLGQNDDGIEATLSDGTTVSADLVVGCDGPH
                     SSVRRLVFGPEAQFVKPLGGYNAWFTAPDTAGLDGWYLMYQAPGLNASMRPSHEPTMC
                     KAGLAFRSEPIGYDRRDADEQRALLAKHFTGAGWQCDALLSAAAEADDFYFDAFAQVH
                     MPSISQGRVTLAGDAGYCASPLSGMGTSLALVGAYVLAGELGAAESLGAERIRAALQR
                     YEAVMRPYIDRCQDLPNGIDGYLPKSASDIAITAQVMSWMQRWPFRYYAQKKWFTTAD
                     AIELPNY"
     gene            complement(6874..7119)
                     /locus_tag="VFC01_04705"
     CDS             complement(6874..7119)
                     /locus_tag="VFC01_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN78979.1"
                     /translation="MATRRVHADEVADELSSHESALRKLPLPYSLALRLRDAGVAPEL
                     VSEYVGVDEAALDGVYRIAEAKLSAAEQSLAPCEAQY"
     gene            complement(7184..9766)
                     /locus_tag="VFC01_04710"
     CDS             complement(7184..9766)
                     /locus_tag="VFC01_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZN78980.1"
                     /translation="MLELGGWPLIGRAEELDAIAGALAKSSESAGVVIFGELGVGKSR
                     LAHDAASRCEGAVVRWAAGSSAARAIPLGAFVNWLPIDVFEPVRATGQLINELIRGTG
                     PRSCRVVVDDAHLLDDTSAFMLQQLVDRRLARLLLTVGTDTAVSDPVAALWRDRPLQR
                     VDLQPLGQSDCLTLLKSVLGGSVDPVSERRLWRLTRGNVSFLRRIVEQETCAGRLRRA
                     DGTWTWLPGAVVPSSVCELIDHQMGDLTDALADTVDLLSVTERLPLGMLEDIVGRETV
                     EHAEMRGLIAVDGREELVVRLAHPLYAEVRRLRAGHIRLRRLRGVVASRLASDGDARH
                     ILRRGTLQLESDLAATPHDMLRAGEAALWSGDTELAVRFAHAAAAAGSGWRASLALAE
                     AQTMAGELDEAETLLAMDDPPRDARLHVALGLSKTFYLHGRTADARAVLDQATLGHPE
                     TTAMHAFLDACSGDFSSAASTADTVLQCTGVDHYSAMLAATVRTIAAGELGRADELAP
                     AAAFARHLGAASPSTGLLRFVLAEAHISASQLLGSTDADDPVDSVRDDDLPPDVFRWV
                     EMMAGSVALSRGRVEDAVPRLRDALSAHNPAFLGGWLCRYHVDLAIALAVCGETDAAM
                     QCLSRLEAARRPETAWLEPMEMLATAWISASTGAVTRAIGEARSAAVTAASRGQPARE
                     VLCLQTATRFGDTTTVARLGELVRVVGGTRARVAARHAAALAASDGDGLMAASAAYEE
                     IGDALAAVDAAAQASVALRQANRRGSALAATNRAHELARRCGDVHTPALSDAAAPAVF
                     TGRMREVVMLAGSGLTNREIAERLQLSVRTVEGHLYRAAGRVGARDRNELARAFGDAA
                     AASA"
     gene            complement(9846..12686)
                     /locus_tag="VFC01_04715"
     CDS             complement(9846..12686)
                     /locus_tag="VFC01_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN78981.1"
                     /translation="MPEDSAEVFSLGEQPLLFDREDDLAALDAALAEAGAGTGGVLLI
                     EGPAGIGKTVLLEALRRRASAAGMAVLTARGGELERGFGFGIVRQLLEGALIGADDAE
                     RDRLLAGAARLAEPVFTDVAAAEDTGDVAFATLHGLYWLVVNLAERAPLLLAVDDAQW
                     ADEPSLRFLLHLAHRLAGLPVIVALTVRSDADRHRQDLGSLMLEARPPVLRPRPLGTP
                     AVARLVRNSLGDNASEELCRACAEATGGNPFLLSELLGEFRRDARPANTIDPRAVDRL
                     APERIAAAVLLRVSRLDPQAPALARAVAVLGEQARLTMCAELAGLDVRKASALAAALV
                     DLAVLDSGEPLRFVHPIVRTAIYNDLSATEQADLHARAAKLLADQRADPGAVAVHLVA
                     TPPSGDRNVVAMLREAARSALAGGAPDTAAEQLRRALDEPPDAHDRPSVLFELGNAEH
                     ELGDPAAPGHLREAGETATDPLIRARALIALAWTTHPNPPKQRDQLPLYEWAADEVRP
                     HDRELALQLDAARLGALMLNPDLPAKLEDEAERVAGLPALTAAECLLRSFVARRALDR
                     GPVNVAGDLAEQAAAHPALVSQGGHPLWRTNVTICLIEAERYEVAEHILSRAARHAER
                     NGSLQWLSRALWLRGLTRIRRGDVRAAEADARAAVDILGTKDYTKVPGLGVVIESLAD
                     QGRVAEAEALLAEYAMDGELVPNLFSVLPLLTRGRLRAAAGDDVRARTDLEEALRWMR
                     LSRGLWPWTTDACVALVPVLRNLGEDDAARAAVEQAMQAATVAQSRRRLGGARRVAGL
                     LEGGKRGLELLREAADTLADSPALLWRAQAFVDLGAALRRDGQVASARLVLRDGMELA
                     HRCGATPLADRAEEELRAAGGRPRRRAGVGADALTASERRVAELAAEGASNKEIAQSL
                     FVTLRTVEMHLSNAYSKLEIRSRHELATALAS"
     gene            12758..13570
                     /locus_tag="VFC01_04720"
     CDS             12758..13570
                     /locus_tag="VFC01_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN78982.1"
                     /translation="MNSAPASPHYPFDNAAPQAGDRFANLSALYDEVTCRHLDRFGIR
                     AGWRCLEVGAGGGSIARIMSQRVGAQGYVMATDINTDWMAPTMPANVDMCRHDIGVDP
                     LPEAAFDVVHSRAVLTFVPERRSALRRMFTALKPQGWLLVEELIPPITEALDSADEPD
                     AEVARKGRTAIVETIRRQGGDPVFAREIPRLMAEAGLSDVGAEGYFVPFRTDAVAALS
                     KANIDQLGPSIVDSGLMTSSELAEFRRLLELPDLLYPASMALISVWGRRQLA"
BASE COUNT         2424 a         4759 c         4310 g         2275 t
ORIGIN      
        1 cggctgaccc gtcaccgcat aactcaacga atcaccctcg ccatcaaccg cattcaacga
       61 caccgacacc acacccgacg actgatccgg cacacccacc accggcgaca ccgacggcgc
      121 agaattcacc accgcagccg ccatcaacat catcggctcc cctgcgccag aatcagccaa
      181 catcattctg gactgcgaag gaccaggcgc catacttccg agagacacgg acacgaggcc
      241 gctgccccca tagctaagga tgtcgccgct ggagtcgacg gcgatggtct gtgatgcact
      301 ggcagaagta tcgctcatgt acccggggaa ccggacggca ccgattacgg cgcgcgtcac
      361 ggcgtcgata accatgatgt cgtcgccgct agcgacatag acgcttccgc catcccggct
      421 gacggcgatg cctgtcggcc agtcggtctg gctgccgctg gccagcggga caccgaggac
      481 gatgggcgtt ccgaccacgg ttctggaggt ggtgtctatt acagcgacgg tgttgtccat
      541 cggattggtc acgtaagccc atttgccgtc ggggctcaac gcaatgccgc ccggggaggg
      601 tgcgatagtt gcagtaccaa gcacgatgga cccgacgacg gccttcgtgt tggtgtcaat
      661 caccggcacc ctgttgttgt atccgtcagc aacgtagatc gtgcgaccgt cggggctcat
      721 caccatgccc cttgtgtccg aactgatggg gatggagccg atgacggtcc tgctatccgt
      781 atcgatgacg ctcatcgatt ggacccatcc atcgtgggcg acgtagaggc gcttaccgtc
      841 aggactcaac accatgtcgt ccgcaccacc ctgtacgggg atcggctcgc cgacgggcgt
      901 gagtgtattg gggtcgacca caacaacttt gccctggagc caattcgcca cgtacaggcg
      961 tgtgccgtcc ggactgaccg ccagattggc cggatagaac tctgacgacg ggaccagccg
     1021 cgatgacgtg gcgataacgg tattcgtatt ggtgtcgatc gctgacaccg tgccgtagta
     1081 ctcgttgacg acgtaggctc gcgagccgtt cacggcaatc gttgggccag aagcagaaag
     1141 cggaatctgt gtctgggtcg acgccagttc tggaggacgg acgggtaccg tcaccgtgaa
     1201 ggtggcggaa ccttgaccgt cggtcgctga caccacgaag gtgtcgagtc cgccggcact
     1261 gcgctgcgac gcgaatgggg tgtaggtata caccccggtc acggagtcga cggtggccgt
     1321 cccacgcgtc gggaggccag ccatagagaa ggtgattctg tctccattgg gatcgtaaac
     1381 gttcatcgac cccgaaactc ggccggtagc cgggtcagcc gatggcacct ccggtggccc
     1441 atacgcgacg ggcgccatct ggatcggcat gacttgtagg ttgacccacg ggctcacgct
     1501 cgccgtgccg tcgctgatgg tcactcggaa ggtgtcgtaa tcggcgtacc cggggtacaa
     1561 cgtcgcggat tcgcgcatct cgcgcgacgg gatataggtg aagacgccag tcgagctgtc
     1621 gagcgaaaaa gttgtcgccc catagggacc ccacagcagt tgataactga gctgatcacc
     1681 gtcaacgtcg ctgacggtga ccctgccccg cacctcacca gtgacagggt cggtggcgta
     1741 gaaatactga aactccgggg ctgttggcgc atggttaccc acggacagaa tcggcacggt
     1801 gacgttggcg gtggtggtgg aggtgttgta cgtgtcggtg gcgcggatgg tgaaggtgtc
     1861 ggtggtgggt ccgccgttgg cggcggcgtc gcgggcggcc gcggtcgggg tgtaggtgta
     1921 gctgcccgtg gccgggtcgt aggtcagcga tcccttggtc ggggccacca cggtggtgta
     1981 ggtcagcggg tcgccgtccc agtctttgat gttcaccaac ccggtaacgg caccggtcga
     2041 tgagtccggc tctccgacag tgggattggc gatcgccacc ggtgcggtgt tgccgcgctg
     2101 ataggccacc acccgcagcg cgcgatcggc caaatcggtg accaccaggg cgccatcggc
     2161 gcgcaccgcc agggtgtggt agttggtctc cggtgcggta tcgatctgaa tggtgttgat
     2221 caccgcacga gtcttggtgt cgatcacact gatccggtca gtgccattgg ccacatacaa
     2281 caggctgccg tccttggata gccccacccc gaccggagtc gagggcaccg caatcgcatc
     2341 caccgtcgac ggtgtggccg gattagcatc aacaaccttg ttggtcgcgg tgtcgatgat
     2401 cgacacactg ttagacccat aattggtcac atacgcccgg gtgccatccg gggacaccac
     2461 gatcaactgc ggcgtggtac ccaccttgat cgcatccacc gtggtgggcg tggccggatt
     2521 ggcgtcaacc accttgttgg tcaccgcatc gatcaccgac accgtgccac cggtgcgatt
     2581 caccacatag acccgtggcc cattgaccgt ctcggcgaac gcgatcccac gcggattaga
     2641 ccccaccgta atcgcatcca ccgtcgtggt ggtggccgga ttcgtgtcga tcaccttggg
     2701 cgccaccgga ttgctgatat caatcaccga caccgtgttc gacgcataat tggccacata
     2761 caaccgcgcc ccggccgcga tctccccagt cgcggtggtg atcgtgctgg tgttgatcgc
     2821 caacatctcc ggctgagacc ccaccttgat cgcatcaacc gtcgtcgggg tggccggatt
     2881 gatatcgatc accttgttat cggaggtccg gatcaccgac accgtgcccg aattacggtt
     2941 ggacacaaac acatacgccc catccaacga ggccaccacc ccagtcgggg ccgaacccac
     3001 cacaatcgga ctaccaaccg cctgcccggt caccgtattg atcaccgaca ccgtattggc
     3061 gccctgattg gccacatacg ccgtcgtgcc aaccaccgcc accccatacg ggctaacccc
     3121 agtcacattc gacgacgacg actgattagt ccacaccgcc ggcaacaccg gcacactcac
     3181 actcaccggc gtactggtct caccatcaga gaccgacacc gtgaacgaat caaaatccgg
     3241 cacactcgtc gaccccgccg ccaaccgcgc caccgccgtc ggcgtatacg tcaccacccc
     3301 cgcacccgac accgacacag tgccactcac cggctgaccc gtcaccgcat aactcaacga
     3361 atcaccctcg ccatcaaccg cattcaacga caccgacacc acacccgacg actgatccgg
     3421 cacacccacc accggcgaca ccgacggcgc agaattggcc accacagcag ccatcaacaa
     3481 cgacccagac ggctcagcca ccttcagcgt cgactcctca tcccccacca gcgactgctg
     3541 ggtctgccgc cggaacgccg ccaaacccgc caataacaac ggcgactcgc ccggcacctc
     3601 cggagcatca tcgtcggccg atggcgccag cccgaccgcc gacaacaccg tcgacgacaa
     3661 cggcctcaca tccggttcag gcgccggctc aaccttgggc aggtcctgca ccgtggaagc
     3721 gctcgatgac gcggattgca gcccgacgtc taccgacacg ctgcccaccg ttggcggcgc
     3781 catcggctcg gacttcggct gtactgccgg accgctgtcc gccgccggct ctcccttgcg
     3841 gacatcgctg tccgaaggtg tgtgcgccac cgcccgcttc ggcttcttcg gcgtcaccgc
     3901 agcggtcgac gcatccgaac cggacttctt gtccttgacc gtctcggact tcttgtccgt
     3961 ctcgctggtg tcactgccct tggattcctc atcgccagcg gcaccgtcac tgtcgtcacc
     4021 gctcgcctcg tcatcgccgg acgcgtccgg ggtcttgggg ctgtccgaag aggaaggtga
     4081 ctgagtgtct gttcccggtg acgatgaggg cgagcccgcc gccgaaccgg aaggggccga
     4141 tggactaccc ggtgaaggcg aattcgacga atccggatcc gacggaccag ccaacgccac
     4201 ccccggcgtg gtcgccaccg caaccccgat ccccaaggcc acagccaagc caccgacacg
     4261 accgacgtac ctggcgtaac ccatcacgac cacctcccag aacggacgcg aatatgcagc
     4321 tcaagccgca agaacatcat cagtccgtcg tccgcggcgg gattaggtag tggagtactc
     4381 gattacgcaa agcaacttag aaggcctatg caattgggct tgggtacgct gtcgccgatg
     4441 aagctcgaca ccaatcaggc ctccattcgc gaggcgatcg acgccggcct gcttgcgggt
     4501 gcggtcacgc tggtttggcg gtctggcgag gtgctgcagg tcaacgagct cggccaccgc
     4561 gacgtagatg ccaatctcga catgcagcgg gacacgatct ttcgcatcgc ctcgatgacc
     4621 aaaccagtga ccgtcgccgc cgcgatgacc ttggtcgacg agggcaagct cgccctgacc
     4681 gatccggtcg cgaagtggtt gcccgagctg tcggacctac gggtgctcga cgctccaggc
     4741 ggcccactgg acaagacgca tccggcgcgc cgcctgatca ccgtcgaaga cctgatgacc
     4801 caccgcagcg gactggccta cttcttctcg gtgaccggcc cgttggcgaa ggcgtactca
     4861 cgcatctccg cgcggcagaa cccggaccgc tggttggccg aggtagcggc gctcccactt
     4921 cagcatcaac ccggcgaccg gatgacctac agcaacgcca ccgacgtgtt gggcatcctg
     4981 ctagaacgca gtgaaggcaa gtctcttcag gacgtgctga ccgagaggat cctcggtccg
     5041 ctgaacatgg ccgacaccgg attctttgtg gcgtcccgca accggggccg tgccgccacg
     5101 atgtacaagc tcgaagacga cgacacactc agccatgacg cgatggggcc gccaccgatt
     5161 gcggcgccgc cgttctgcat gggcggcgcg aatctgttct ccaccgccga cgactatctg
     5221 acgtttacgc gaatgctgct cggcggcggc gaagtcgacg gtgtacgggt gctttccgaa
     5281 aagtcggtcc ggctgatgcg caccgaccga ctgacggacg agcagaaacg tcacccgttt
     5341 cttggtatgc cgttttggat cggccgtggc ttcggcctga acttgtcggt ggtgaccgac
     5401 ccggcgaagt cacgtcaatt gttcgggccc ggcggcctgg gcaccttcag ttggcccggc
     5461 gcctacggca catggtggca ggctgatccg agcgcgaacc tcatcctgat ctatctcatc
     5521 cagaacctgc caaacttcgg cgccgacgcc gcggccgccg ttgcaggcaa cacctcattg
     5581 ctgaaacttc agtccacgca accgaaattc gtgcgccgga cctatcaggc gctcgacatc
     5641 tagcgactac ccttgcgctc atggcagcgc cgaccgtcct gatcagcgga gcaggaatcg
     5701 cagggcctgc acttgccttc tggctcaaca agagtgggta tcgcgtcatc atcgttgagc
     5761 tcgcggatgg cattcggccc ggcggacaaa ccgtcgactt gagaggcgcc ggcggcgacg
     5821 tggtcgagca catgggcctg atcgagcaga tgcgccagcg tgcgctcgat cagcgtggcg
     5881 tggcctggat caaatccgat gggcgccgcc gagcggagat gccggtgacg gcgttcgacg
     5941 gcaacgggtt ggtctccaag ctcgagatct tgcgcggcga cttggtcgat gtgctctatc
     6001 aggcgaccaa ggaccagacc gactaccgct tcgacacccg gatcgaggat ctcggccaga
     6061 acgacgacgg tatcgaggca acattgagcg acggaacgac agtgtctgct gatctggtcg
     6121 tcggttgcga tggtccccat tcctcggtgc gccgcttggt gttcggcccc gaagcgcagt
     6181 tcgtcaagcc gctcggcgga tacaacgcgt ggttcactgc accggacacg gcagggctcg
     6241 acggctggta cctgatgtac caagcgcccg gcctcaacgc gtcgatgcga ccgtcgcacg
     6301 agccgacgat gtgcaaggcc gggctggcat ttcgctccga acccatcggc tatgaccgcc
     6361 gcgatgccga cgagcaacgg gccctgctcg caaagcattt cacgggcgcc ggttggcaat
     6421 gtgacgccct gctctcggcg gccgccgagg ccgacgattt ctacttcgac gcgttcgcgc
     6481 aggtgcacat gccgtcgatc tcacagggtc gagtcacgct tgccggtgac gccggatact
     6541 gcgcctctcc gctcagcggc atggggacca gcctcgcgct cgttggtgcg tatgtgctcg
     6601 ccggcgagct tggcgctgct gaatcacttg gcgccgaacg gatcagggca gcgctgcagc
     6661 gctacgaagc ggtgatgcgg ccctacatcg accggtgtca ggatctcccc aatgggatcg
     6721 acgggtatct gccgaagtcc gccagcgaca tcgcgatcac tgcgcaggtg atgagctgga
     6781 tgcagcgctg gccatttcgc tactacgccc agaagaaatg gttcaccacg gccgacgcca
     6841 tcgagctacc gaattactga gccccgcccg tcgtcaatat tgcgcttcgc atggcgccag
     6901 tgattgttcg gcagcgctga gcttcgcctc ggcgatgcgg tagacgccgt ccagtgccgc
     6961 ctcgtcgacc ccgacatact cgctcaccag ctcgggcgcg acgcccgcgt cgcgtaaccg
     7021 cagcgccagg gagtacggca gcggaagctt gcgaagtgcg ctctcatggg agctgagttc
     7081 atcggccacc tcatcagcgt gcacccgccg ggtggccacg gggtgagtac gcctctactc
     7141 gatttttcgc gtacccgggc cccagcccac gcaaccgcga ggcctatgcc gatgccgccg
     7201 ccgcatcgcc gaacgcccgt gccaactcgt tgcggtcgcg ggcaccgacg cgtccggccg
     7261 cccggtagag gtgtccctcc acggtgcgca cggacaactg aagccgctcg gcgatctcgc
     7321 ggttggtcag tccgctgcct gccagcatga cgacctcacg catccggccg gtgaataccg
     7381 ctggagcggc ggcatcgctc agcgcaggcg tgtggacgtc gccgcaccgg cgggccagtt
     7441 cgtgcgcccg gttcgtcgcc gccaacgcgg atccccgccg gttggcctgt cggagcgcga
     7501 cggacgcctg agcggcggcg tcgaccgcgg ccaacgcgtc gccgatctcc tcatatgccg
     7561 ccgaggccgc catcagcccg tcaccatcgc tcgccgccag cgcagccgcg tgacgggcgg
     7621 ccaccctggc gcgagtaccg ccgaccaccc gaaccaactc ccccagccgc gcgacggtcg
     7681 tcgtgtcccc gaaccgtgtc gcagtctgca ggcacagcac ctcacgcgcc ggctgtccgc
     7741 gtgacgcagc ggtcaccgcg gcgcttcgcg cctccccgat ggcgcgtgtg accgcaccgg
     7801 tcgacgccga aatccatgct gtcgcaagca tttccatcgg ttccagccac gcagtctccg
     7861 gcctgcgagc agcttcgagt ctgctcaggc actgcattgc cgcgtcggtc tcgccgcata
     7921 cggccaacgc gatggccagg tcgacgtggt agcggcagag ccagccaccc aagaaagcgg
     7981 ggttgtgtgc cgacagtgca tcacgcaacc gcggcacggc atcctcgacg cggccacgac
     8041 tgagggctac cgaaccggcc atcatctcga cccagcgaaa gacgtcgggc ggcagatcgt
     8101 cgtcacggac gctgtcgacc ggatcgtccg cgtccgtcga tccgagcaat tgcgacgcgg
     8161 agatgtgcgc ctcggcgaga acgaaccgga gtaatccagt cgacggcgac gcggcgccga
     8221 ggtggcgagc gaatgccgcg gccggggcca gctcgtcggc gcggcccagt tcaccggccg
     8281 caatcgtcct taccgtcgcc gcgagcatcg cggagtaatg gtccacgccg gtgcattgca
     8341 ggacagtgtc ggcagtcgac gccgccgagg agaagtctcc gctgcacgcg tcgaggaacg
     8401 cgtgcatcgc agtcgtctcc gggtgcccca gcgttgcctg gtccagaacc gcacgcgcgt
     8461 ctgcggtccg accgtgcaaa tagaaggtct tcgacaggcc gagtgccacg tggagtcgag
     8521 cgtcgcgcgg cggatcatcc atggcgagaa gcgtttccgc ttcgtcgagt tcgcccgcca
     8581 tggtctgcgc ctcggccagg gcgagcgagg cgcgccatcc gcttccggcc gccgcagcgg
     8641 cgtgcgcgaa ccggaccgcc aactcggtat cgccgctcca gagcgccgcc tcgcctgcgc
     8701 gcagcatgtc gtgcggcgtc gctgcgaggt cggattcgag ctgcagcgtg ccgcgacgca
     8761 gaatgtgcct cgcatcgccg tcagacgcca ggcgcgaggc gaccacaccc cgcagccggc
     8821 gcaaccggat gtggccggcc ctcagccgac gaacctccgc atagagcgga tgagccagtc
     8881 gcacaacgag ttcctcgcgc ccgtcgacgg cgatcagacc gcgcatctcg gcgtgctcca
     8941 cggtctcgcg cccgacgatg tcttccagca tccccagggg caaccgttct gtgaccgaaa
     9001 gcaggtcgac cgtgtcagcg agcgcatccg tcaagtcacc catctggtga tcgatcagtt
     9061 cgcagaccga cgacgggacc acagcgcccg gtagccatgt ccatgtcccg tcggcacggc
     9121 gcagacgtcc agcgcacgtc tcctgttcga cgatgcgccg caggaagctc acattgccgc
     9181 gagtcaggcg ccacagccgg cgctcgctga ccggatccac cgacccaccc aggacagact
     9241 tgagcagggt caaacagtcg gactgcccca gcggctgcag gtcaacgcgc tgaagcggcc
     9301 ggtcgcgcca cagcgcggcc acgggatcgg atacagccgt gtcggtgccg accgtgagca
     9361 gcaggcgcgc caggcggcgg tcgacgagct gctgcaacat gaacgccgag gtgtcgtcga
     9421 gcagatgggc gtcatcgacg accacgcggc aggaccgcgg ccccgtgcct ctgatgagtt
     9481 cgttgatcag ctggcccgtt gcccgcaccg gctcgaagac gtcgataggc agccagttga
     9541 cgaacgcacc aagcggaatc gcccgagccg ccgacgatcc cgctgcccac cgaacgaccg
     9601 cgccttcgca tcgcgaggca gcatcgtgag cgagcctgct cttgccgaca cccagctccc
     9661 cgaaaatcac cacgccggca gattcactgg acttcgcgag cgcccccgcg atcgcatcca
     9721 attcctcggc tcggccgatc agcggccagc cccccagttc caacacactg tttagtctgg
     9781 tcggagctac ttttgctgac taccgtatcg gcccgaagca ttgaccgaag tcttttcccg
     9841 cgccgtcacg acgccagcgc tgtggccagt tcgtggcgtg atcgaatttc aagcttgctg
     9901 tacgcattgg acagatgcat ctccaccgtt cgcagggtga cgaacagcga ctgcgcgatc
     9961 tccttattgc tggccccttc ggccgccagt tcggcgactc gccgctcact ggcggtcaac
    10021 gcgtccgctc ccactcctgc ccgtcgtcgt ggtcggccgc ccgcggcgcg caactcttct
    10081 tcggcccggt cggcgagcgg tgtggcgcca catcggtggg caagttccat gccgtcacgc
    10141 aggactagcc gcgccgaggc gacctgcccg tctcgccgca acgcggcgcc cagatcgacg
    10201 aatgcctgtg cccgccataa caaggccggc gaatcggcca acgtgtcggc cgcctcacga
    10261 agcagctcca gtccacgctt gccgccctcc agcagtcccg cgacccgccg ggcgccgccg
    10321 agcctgcgcc gagactgtgc gaccgtcgcc gcttgcatcg cttgttcgac ggcagcgcgg
    10381 gcggcgtcat cctcgccgag gttacgcagc acgggaacga gcgccacgca agcatctgtt
    10441 gtccacggcc acagcccacg gctcaagcgc atccacctca acgcctcttc gaggtcggtc
    10501 cgcgcacgaa catcgtcacc cgcggcagcg cgcagccgcc cacgagtcag taagggcaag
    10561 accgagaaca gatttggcac gagctcgccg tccatcgcgt attccgcgag caaggcttcg
    10621 gcttcggcga cgcggccctg atcggccagc gattcgatca ccacgccaag gcccggcacc
    10681 ttggtgtagt ccttggtccc gaggatgtcg accgcggcgc gggcatccgc ttctgccgca
    10741 cgcacgtcgc cgcggcgaat gcgggtcaga ccgcgcaacc acagcgcacg cgacaaccac
    10801 tgcagcgaac cgttgcgctc ggcgtggcgc gcggcacgag aaagaatgtg ttcggcaacc
    10861 tcgtaacgct cggcctcgat caagcagatc gtgacgttcg tccgccacaa cgggtgaccg
    10921 ccctggctga cgagtgcggg atgggccgct gcttgttcgg ccagatcgcc cgccacattc
    10981 acgggtccac ggtcgagtgc cctgcgggcg acaaaggacc gcaacaggca ctctgctgcc
    11041 gtcagcgccg gcaagccggc gacgcgctcc gcttcgtcct cgagtttggc gggcaggtcc
    11101 ggattgagca tgagtgcgcc caacctcgcc gcatcgagct gcagtgcgag ctcacgatca
    11161 tggggtcgga cttcgtcggc ggcccactcg tagagcggca gttggtcacg ctgtttgggc
    11221 gggttcggat gcgtcgtcca cgccagggcg atcaatgcgc gcgcgcgaat cagtggatcc
    11281 gtcgcggtct cacccgcttc ccgaagatga ccgggagcgg ctgggtcccc caactcgtgt
    11341 tctgcgttgc cgagttcgaa cagcacggat ggccgatcgt gagcatccgg tggctcgtca
    11401 agtgcccggc gcagctgctc cgccgcggtg tcgggggcgc cgccggccag cgcggacctg
    11461 gcggcctccc gcaacatcgc aacaacgttg cggtctccgc tcggcggcgt cgcaacgagg
    11521 tgcaccgcca ccgcacccgg atcggcccgc tggtcggcca gcagcttggc cgcccgcgcg
    11581 tggagatcgg cctgttcggt ggcggataaa tcgttgtaga tcgctgttcg cacgatcgga
    11641 tgaacaaatc gcagcggctc cccgctgtcg agcaccgcga ggtcgaccaa ggcggccgcc
    11701 agcgcgctcg ccttccttac gtccaacccg gccagctcgg cgcacatcgt cagtcgggcc
    11761 tgttcgccca gcaccgcgac tgcccgcgcc agagcgggcg cctgcgggtc gagtcggctg
    11821 acgcgaagca gcacggcagc agcgatgcgc tcgggcgcca acctgtcgac cgcccgtggg
    11881 tcgattgtgt tcgccgggcg cgcgtcgcgg cggaactcgc cgagcagttc ggacaacagg
    11941 aacgggttgc caccggtcgc ttcggcgcac gcccggcaca actcttcact tgcgttgtcg
    12001 ccgaggctat tccgaacgag acgcgcaacc gccggcgttc ccagcggccg ggggcgaagg
    12061 acaggcgggc gggcctccag catcagcgat ccgaggtcct gccgatgccg gtcggcatcc
    12121 gatcgcacgg tcagcgcgac tatgaccggt aggcccgcga gccgatgtgc gaggtgaaga
    12181 aggaaccgca gcgacggctc gtcggcccac tgcgcatcgt cgacggcgag aagcagcggc
    12241 gcccgctcgg cgaggttgac caccaaccag tacagcccgt gcaaagtggc gaacgcgacg
    12301 tcgccggtgt cctcggccgc cgcgacatcg gtgaagacgg gctcggccag gcgcgcggca
    12361 cccgccaaca agcgatcgcg ttccgcgtcg tcagcaccga tcagcgcgcc ttcgagcagc
    12421 tggcggacga ttccgaagcc gaacccgcgc tccaactccc cgccgcgggc ggtcagcacg
    12481 gccatccccg cagcgctggc gcgacgacga agcgcctcca gcagcaccgt cttcccgatg
    12541 cctgcgggcc cctcgatcag cagtacaccc ccggtccctg cgccggcttc ggccagcgca
    12601 gcgtcgagcg cagcgaggtc gtcctcgcgg tcgaacagca gcggctgctc ccctaaagaa
    12661 aagacttcgg ccgaatcttc gggcatcaac tctgtcgtag tccccccggt tcgttagacc
    12721 atcttctaca tgagagcacc tcggtggtga accggtcgtg aattccgccc cggcgtcgcc
    12781 gcactatccc ttcgacaacg cagcgccgca ggccggcgac cgcttcgcga acctttccgc
    12841 cctgtacgac gaggtgacgt gtcgccacct ggaccggttc ggaatccgcg ccggttggcg
    12901 atgcctcgaa gtcggtgcgg gcggcgggtc gatcgcgcgc atcatgagcc agcgggtcgg
    12961 cgcgcagggt tacgtgatgg cgaccgacat caacaccgac tggatggccc cgacgatgcc
    13021 cgccaatgtg gatatgtgtc gccacgacat cggcgtcgac cccctgcccg aggcggcgtt
    13081 cgacgtcgtc cattcccgcg cggtgctgac cttcgttccc gagcgacgat cggcgttgag
    13141 gcgcatgttc accgcgctca agccgcaggg ctggctactc gttgaggaac tgatcccgcc
    13201 gatcacggag gcgttggatt cagccgatga gcccgacgct gaggtggcgc ggaagggccg
    13261 cactgcgatc gtggagacga ttcggcgcca gggcggcgat cccgtgttcg cgcgggagat
    13321 cccgcgactg atggccgagg ccggcctgtc cgacgtcggc gccgagggct atttcgttcc
    13381 gttccgtacg gacgcagtgg ccgcgctctc caaggcgaac atcgaccagc tcggtccctc
    13441 gatcgtcgat agcgggttga tgacgtcgtc ggagctggcc gagttccgcc gacttctcga
    13501 gctgcccgat ttgctctatc ccgcgtcgat ggcgctgatt tccgtgtggg gacggcggca
    13561 actggcataa cgttcgagct catggaccag gggggtaggt gccggagctg atgcgacgcg
    13621 ggaagctgat tcgtctgttg gcgaatgtgc ttgcgtcact gacgcttgtg ctcgcgggtt
    13681 gtggcacggg ctctgcgccg cacaccgacc agcagctgac cctgttgcgg gtcgcgatgt
    13741 ttcccgccgg cagcacgctt cccgcgca
//
LOCUS       DASRJA010000103         7582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1691553,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000103 DASRJA010000000
VERSION     DASRJA010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691553"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2398
                     /locus_tag="VFC01_04725"
     CDS             <1..2398
                     /locus_tag="VFC01_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZN78983.1"
                     /translation="AICALGAGVDERLAAGDVRLTVGGEPTFVSIDNQVDPEWTTDAD
                     GPHKRQRASALAARLKKVWAPQGLIQHSQGKWYPGEPLPRWQIGLFWRTDGEPLWRDE
                     KLLADPWPSGAAEAFAIASDAGAQVLGAVADGLGLPASQVRPAYEDALSRLAGTVRQP
                     AGEPVASEDDLADDAADRRKALLARLEELVTEPAAYVLPLHRRDDDSGWASADWQLRR
                     GRIVLLEGDSPAGLRLPLNSISWHPPEPPFQADPLARRGRLPVAPEPGDAPVEDVEEA
                     DAVPVTAMVAEIRDGLLYIFVPPTEALEDFVDLIARIEAAAAKVDCQVVVEGYGPPPD
                     SRLQSMSVTPDPGVIEVNVAPTPSFAAQKAQLETLYEQARLARLSTEAFDFDGTHGGT
                     GGGNHITLGGITPADSPLLRRPDLLVSLLTYWQRHPSLSYLFAGRFVGTTSQAPRVDE
                     GRSEALYELEIAFAEIARLSGGEGVRKGTAKPWITDRALRHLLTDITGNTHRAEFCID
                     KLYSPDSARGRLGLLELRGFEMPPHYQMAMVQSLLVRALVARFWDEPLRAPLIRHGAN
                     LHGRYLLPHFLIHDIADVAADLRAHDINFDTSWLDPFTEFRFPRIGTAVFDGVEIELR
                     GAIEPWNVLGEESTAGGTARYVDSSVERIQVRLIGADRHRYVVTANGYPIPLLATDNP
                     DVQVGGVRFRAWQPPSALHPTITVDGPLRFELVDTVTGMSRGGCTYHVSHPGGRAYDA
                     PPVNAVEAESRRGRRFDATGFTAGKVDMSDIFEKQARQSTDVGAPGILDLRRVRTVLQ
                     "
     gene            2398..5037
                     /locus_tag="VFC01_04730"
     CDS             2398..5037
                     /locus_tag="VFC01_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZN78984.1"
                     /translation="MALPAPDTHDIDHLLARYRTARAQQALFDVRGAGAGTGYDEFVD
                     GAGNVRPAWQELAECVGERGRGGLDQLRSIVRGLVDNDGITYIEVDRHGDAVTNGDGT
                     AEPGPWHLDALPLVLSSADWDTLESGLVQRSRLLDAVLSDLYGPRHAITSGVLPPQLL
                     FAHPGYVRAARGIEVPGRHQLFLHGCDISRAADGSFRANADWTQAPSGAGYALADRRV
                     VAHAIPDLYERIGPRPASPWAQALRLALIDAAPESAEEPVVVVLSPGIHSETAFDQAY
                     LASVLGFPLVESADLVVRDGKLWMRSMGTLKRVDVVLRRVDAEYADPLDLRADSRLGV
                     VGLVEVLRRGAVTVVNTLGSGILESPGLLRFLPQLAERLLGETPLLQTAPMYWGGINT
                     ERSHLLTNLSSLLVKSVSGGEAIVGPALSSAQRKALGVRIEATPWQWVGQELPQFSSA
                     PSDYYQGGLSSASVGMRLFTVSQRSGYAPMIGGVGYLLAPGNAAYRLNTVAAKDIWVR
                     TPTRVTVEGIPSAVELPAMTPSPTHAVSSPRVLSDLFWMGRYAERAENMARLLTVTRE
                     RYHEFRYRSEMEGRECVPVLLAALGHITGTDTGVGGDDAEMIATAQTTLWSLTADRHR
                     AGSLAQSVERLGLAARAVRDQMSNDTWMVLAAVERALLHAPDSPPESQTEGDAFLSST
                     NNLTLAGMLALSGVAAESMVHDVGWTMMDIGKRIERGLALTALLRATLTTVRSPGAEQ
                     TITESTLVACESSVIYRRRTLGKVSVAAVADLVLFDTENPRSLVYQLERLRANLKALP
                     GSSGSSRPERLVDEIAIRLRRLDPADLEDVTADGRRAELADLLNGMHRDLRELSGVIT
                     ATHLSLPGGMQHLWGPDEFRVVP"
     gene            5034..5957
                     /locus_tag="VFC01_04735"
     CDS             5034..5957
                     /locus_tag="VFC01_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZN78985.1"
                     /translation="MTDGPITSPSSRCYQIAHHTVYRYSDVVTSSYGRGFLIPRDSAR
                     QRCLSHELLIEPEAADGSTSRDVYGNVSSYFHVTERHHTLSITSNSVVEVDPPPPELY
                     GGGSARAPWEIARPVGVDGALATEFTLDLQPPEITDELRAYAAPSFEPGRPLIDVLRD
                     LTSRIYRDFTYQSGSTTVSTQVSEVLAAREGVCQDFARLAIACLRANGLAASYVSGYL
                     ATDPPPGKERMIGIDATHAWASVWTPQNQWLGLDPTNDQMVDERYIVVGFGRDYADVP
                     PLRGIIYTDSERSVIDVSVDVAPYEGGVLRA"
     gene            5950..6999
                     /locus_tag="VFC01_04740"
     CDS             5950..6999
                     /locus_tag="VFC01_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HZN78986.1"
                     /translation="MRDFKCPNCGQRLAFENSLCLNCGSRLGFSLDDTALLVIASGAD
                     SDHGGAVDSSEYQLCANLHLAECNWLVEKGPVTRLMCRSCALTRTRPNDSDTKAMSAF
                     AAAEKAKRRLIFELYELKLPIVGRDEDPDYGLAFDLLSSELEKVMTGHHNGVITLDLA
                     EGDDVHREQLRVEMDEPYRTLLGHFRHEIGHYYFYRLVGLSQAYSQRFRELFGDPDGD
                     YQEALDRHYNDGPPAGWKDDYVSSYASMHPAEDWAETFAHYLHIRDALDTAAAFGFAQ
                     AAATFERRALGPSGFDTIIDMWLPLAWALNMVNRSMGKEDLYPFVLPPAVLEKMRFIH
                     TIIDEITSDPAKLTA"
     gene            complement(7145..>7582)
                     /locus_tag="VFC01_04745"
     CDS             complement(7145..>7582)
                     /locus_tag="VFC01_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZN78987.1"
                     /translation="FDGVGAGMAVEYSPLGAIPSIRDGMEYVRAASRTARAGLLVDSW
                     HFCFSDDTWDDLAAVPLEDMAYVQFTDALEPEYRDRMVRESLHRRALPGEGILELDRF
                     VATFLDRGWDGTVSVEVLSAQLRELSVDELVGRLYTTTAAYWS"
BASE COUNT         1213 a         2547 c         2561 g         1261 t
ORIGIN      
        1 tgcgatctgc gcgctggggg ccggtgtcga cgaacgtctg gccgccggtg atgtccgcct
       61 taccgtgggt ggcgagccga cgttcgtgtc gatcgacaat caggtcgacc ccgagtggac
      121 caccgacgcc gacgggccgc acaaacggca gcgcgcctcg gcgctggcgg cccggctcaa
      181 gaaggtgtgg gcaccccagg gtctgatcca gcacagccag ggcaagtggt atccgggaga
      241 acccttgccg cgctggcaga ttgggctgtt ttggcgcacc gatggcgaac cgctgtggcg
      301 cgatgagaag ctgctggccg atccctggcc ttccggagct gcagaggcgt ttgcgatcgc
      361 tagtgacgcc ggggcgcagg tgctcggtgc cgtcgccgac gggctcgggc tgccggcctc
      421 gcaggtgcgg ccggcctacg aggatgcgct gagcaggctt gccggtacgg tgcggcagcc
      481 cgcaggtgag cctgttgcgt cggaggacga tctggccgac gatgccgcag accgtcgaaa
      541 ggcactgttg gcccggctcg aggagcttgt caccgagccc gccgcctacg tgctaccgct
      601 gcatcgccgc gacgacgatt caggctgggc cagcgccgac tggcagctac ggcgcggccg
      661 aatcgtgttg ctcgagggcg actcgccggc cgggttgcgg ttgccgctga actccatcag
      721 ctggcacccg cctgagcccc cgttccaagc cgacccgctc gccaggcgtg gcaggctgcc
      781 ggtggcgccc gaacccggtg acgcgccggt cgaggacgtc gaggaggccg atgccgttcc
      841 tgtgacggcg atggtcgccg aaatccgcga cggcttgctc tacatcttcg tgccgcccac
      901 cgaagcgctg gaggacttcg tcgacctcat cgcccgcatc gaggccgccg cggcgaaggt
      961 cgactgccag gtcgttgtcg aagggtacgg gccaccgccc gattcgcggc tgcagtcgat
     1021 gagcgttacc cccgaccccg gtgtcatcga ggtcaacgtc gcaccgacgc ccagcttcgc
     1081 cgcgcagaag gcgcagctgg aaaccctcta cgagcaggcc cggctcgctc gactgagcac
     1141 cgaggcattt gacttcgacg gcacccacgg cggtaccggc ggtggcaacc acatcacact
     1201 cgggggaatc accccggcgg actccccgct gctgcgccga cccgacctgc tggtatcgct
     1261 gctgacctac tggcagcgtc acccgtcgct ctcctacctg ttcgccgggc ggtttgtcgg
     1321 caccacatcg caggcgccac gagtggacga gggccgctcc gaggcgctct acgagctgga
     1381 gatcgcattc gccgagatcg cgcggctttc gggcggcgaa ggcgtccgaa aaggcactgc
     1441 caaaccgtgg atcaccgatc gggcgctgcg ccatctgctg accgacatca ccggcaacac
     1501 ccaccgggcc gagttctgca tcgacaagct ctacagcccg gacagcgccc gcggccggct
     1561 cggcctgctg gagctgcgcg gcttcgagat gccgccgcac tatcagatgg cgatggttca
     1621 gtcgcttctc gtgcgcgcgc tggtggcccg gttctgggat gagccgctac gggccccact
     1681 catccgccac ggcgccaatc tgcacggccg atacctgttg ccgcacttct tgattcacga
     1741 tatcgccgat gtcgccgccg atctgcgcgc gcacgacatc aacttcgaca ccagctggct
     1801 ggacccgttc accgaattcc ggttcccgcg cattggcact gcggtgttcg acggcgtcga
     1861 gatcgagctg cgcggcgcca tcgaaccctg gaacgtgctc ggcgaggaat ccaccgctgg
     1921 cggcaccgcg cgctacgtcg actcgtcggt ggaacgcatt caggtccggc tgatcggcgc
     1981 cgaccggcac cgctacgtcg tcaccgccaa cggctacccg atcccgctgc tggccaccga
     2041 caaccccgac gttcaagtcg gcggcgtgcg gttccgggcg tggcagccgc ccagcgcgct
     2101 gcacccgacc atcacggtcg acgggccgct gcgcttcgag ctcgttgaca ccgtcacggg
     2161 gatgtcgcgc ggcggctgca cctaccacgt gtcccacccg ggcggccgcg cctatgacgc
     2221 tccgccggtc aacgccgtgg aggccgagtc ccggcgaggt cgtcgctttg acgcgactgg
     2281 tttcaccgcc ggaaaggtgg acatgtcgga catcttcgag aagcaggccc gccagtccac
     2341 cgacgtgggc gcgccgggca tcttggatct acgccgagtg cgtaccgttc tgcagtgatg
     2401 gctctacccg cacccgatac gcacgacatc gaccacctgc tcgcccggta ccgcacagcg
     2461 cgggctcagc aggcgctctt cgacgtgcgt ggcgccggcg ccggtaccgg atatgacgaa
     2521 ttcgtcgacg gggcaggcaa tgtccgccct gcctggcagg aactcgcgga atgtgtgggg
     2581 gagcggggcc gcggcggatt ggatcagctg cggtcgatcg ttcgcggcct agtcgacaac
     2641 gacggcatta cctatatcga ggtggaccgg cacggcgacg cggtgaccaa cggcgacggg
     2701 accgctgagc cggggccgtg gcacctcgat gcgctgccgc tggtgctgtc ctcagcagac
     2761 tgggacaccc tggaatccgg tctggtgcaa cgttcccggc tgctcgacgc ggtgttgtcc
     2821 gacctctacg gaccacgcca tgccatcacc agcggtgtgc tgccgccgca gttgctcttc
     2881 gcgcaccccg gctatgtcag ggccgcccgc ggcatcgagg tgccgggccg gcatcaactg
     2941 ttcctgcacg gctgtgacat cagccgcgcc gccgacggca gcttccgggc gaacgccgac
     3001 tggacccaag cgccgtcggg agcgggctac gcgctggccg atcggcgagt ggtcgcgcat
     3061 gccatccccg acttatacga gcggataggg ccgcggccgg cctcgccgtg ggcgcaggcg
     3121 ctgcggctgg cgctgatcga tgccgccccc gaatcggctg aggagcccgt ggtggtggtg
     3181 ctgtctcccg gcatccactc cgagaccgcg ttcgaccagg cgtatctggc gagcgtgctg
     3241 ggcttcccgc tggtggagag cgccgatctg gtggtgcgcg acggcaagct gtggatgcgg
     3301 tcgatgggca cgctcaagcg tgtcgacgtg gtgctgcgcc gggtcgacgc ggagtacgcc
     3361 gatccgcttg acctgcgggc agattcgcgg ctcggcgtgg tcggcctggt ggaggtgttg
     3421 cgtcgcgggg cggtcacagt cgtcaacacg ttgggcagcg gcatcctgga aagccctgga
     3481 ctgcttcgct ttctgcccca actggccgag cggctgctgg gcgagacccc gttgctgcag
     3541 acggcgccga tgtactgggg cggtatcaac acggaacgtt cgcatctgct gacaaacttg
     3601 tcctcgctgt tggtcaagtc ggtgtcgggc ggggaggcca tcgtcgggcc ggcgttgtcg
     3661 tccgcgcagc gcaaagcgct cggcgtgcgc atcgaggcaa cgccgtggca gtgggtgggt
     3721 caggagctac cgcagttctc gtcggcgcca tccgactact accagggtgg gctgtcgtcg
     3781 gccagcgtcg gcatgcggtt gttcacggtg tcgcagcgaa gtggctacgc gccgatgatc
     3841 ggcggggtgg gatatctgtt ggcgcccggc aacgcggcgt atcggttgaa taccgttgcg
     3901 gcaaaggata tctgggtgcg caccccgacc cgtgtcacgg tcgaagggat accgagcgcg
     3961 gtcgagctgc cggcgatgac gcccagcccg acgcacgccg tcagctcgcc gcgcgtgctg
     4021 tccgacctgt tctggatggg ccgctacgcc gagcgggctg agaacatggc gcgtctcctc
     4081 accgtcaccc gcgagcgcta ccacgaattc cgctaccgca gcgagatgga aggccgcgag
     4141 tgtgtaccgg tgctgctggc cgcgctcgga cacatcaccg gaaccgacac gggcgtcggc
     4201 ggcgacgacg cggagatgat cgcgacggcc cagacgacgc tgtggtcgtt gaccgccgac
     4261 cggcatcgcg ctgggtcgct ggcgcagtcg gtcgagagac tcggtttggc cgcccgcgcc
     4321 gtgcgcgacc agatgtccaa cgacacgtgg atggtgctgg cggccgtcga acgcgcgctc
     4381 ctgcatgcac ccgattcgcc gcccgaatcg cagaccgaag gcgacgcgtt cctgtcgtcg
     4441 acgaacaacc tcaccctggc cgggatgctg gccctgtccg gcgtcgcggc cgaatcgatg
     4501 gtgcacgacg tcggctggac aatgatggac atcggcaaac gaatcgaacg cgggctggcg
     4561 ctgacggcac tgctgcgtgc gacgctgacg acggtccgca gcccgggtgc cgagcaaaca
     4621 atcacggagt cgacactggt ggcctgcgaa tcgtcggtca tctaccgccg gcgcacgctg
     4681 ggcaaggtta gtgtggctgc tgtggcggac ctggtgttgt tcgacactga aaacccgcgc
     4741 tcgctggtct accaactgga gcggttgcgg gccaacctga aggcgctgcc cggttcgtcg
     4801 ggatcgtcgc gacctgagcg gctggtcgac gagatcgcca tccggctgcg ccgcctcgac
     4861 ccggccgatt tggaagacgt caccgccgac ggcaggcggg ccgaactggc cgatctgctc
     4921 aacggaatgc accgcgatct ccgcgagctg tccggcgtca tcaccgcgac ccacctgtcg
     4981 ctgcccggtg gcatgcagca tctgtggggt cccgacgagt tccgagtcgt cccgtgaccg
     5041 acgggcccat cacctcgccg tcgagtcggt gctatcagat agcgcaccac acggtgtatc
     5101 gctactccga cgtcgtcacc agctcctacg gccgcggctt cctcataccc cgcgactccg
     5161 cacggcagcg gtgcctctcg cacgaactgc tcatcgaacc cgaggcggcc gacggctcca
     5221 ccagccgtga cgtctacggc aacgtcagct cgtatttcca cgtcaccgag cgccatcaca
     5281 cgctgtcgat caccagcaat tcggtggtcg aggtggatcc gccgccgccc gagctctacg
     5341 gcggcgggtc ggcgcgggcg ccgtgggaga tcgcgcgtcc ggtcggcgtc gacggggcgc
     5401 tggccaccga gttcacgctc gacctgcagc cgccggagat caccgacgag ctacgtgcct
     5461 acgccgcacc gagtttcgag ccgggccggc cgctgatcga cgtgctgcgc gacctgacgt
     5521 cgcggatcta ccgggacttc acctaccaat cgggctcgac gacggtgtcc actcaggtca
     5581 gcgaggtttt ggcggcgcgg gaaggggtat gtcaggactt cgcgcggctg gccatcgcct
     5641 gtctgcgcgc caacgggttg gcggccagct acgtgtccgg gtacctggcg accgacccgc
     5701 cgccgggaaa ggaacgcatg atcggcatcg acgcgacgca tgcatgggcg tcggtgtgga
     5761 cgccgcagaa ccagtggctg gggttggatc ccaccaacga ccagatggtc gacgagcgct
     5821 acatcgtcgt cgggttcggc cgcgactacg cggatgtgcc gccgttgcgc ggcatcatct
     5881 acaccgactc cgagcgcagc gtgatcgacg tgtccgtcga cgtggcgccg tacgaaggcg
     5941 gcgtgctgcg tgcgtgattt caagtgcccc aactgcggtc agcgcctggc gttcgagaac
     6001 tcgctgtgcc tgaattgcgg aagccggctg ggtttctcgc ttgacgacac ggctctgctg
     6061 gttatcgcgt cgggggcgga cagcgaccac ggcggcgcgg tcgactccag cgagtaccag
     6121 ctgtgcgcca atctgcatct ggccgaatgc aattggctcg tcgagaaggg gcctgtcacc
     6181 aggctaatgt gcaggtcgtg cgcactgacc cgaactcgcc ccaatgacag cgataccaag
     6241 gcgatgtccg cattcgcggc cgccgagaag gccaagcgca ggctgatctt cgaactgtac
     6301 gagctcaagc tgccgatcgt gggccgcgat gaggacccgg actacggctt ggcgttcgac
     6361 ctgctgtcga gcgaactcga gaaggtgatg acgggccacc acaacggcgt gatcacgctc
     6421 gatctcgccg agggcgacga cgtgcaccga gagcagttgc gggtggagat ggacgagcct
     6481 taccgcacgc tgctgggcca ttttcgacac gagatcggcc actactactt ttaccggctg
     6541 gtcggcttgt cgcaggctta ctcgcagcgc ttccgggagt tgttcggcga tcctgatggc
     6601 gactatcagg aggcgctgga ccgccactac aacgatggcc cgccggccgg ctggaaagac
     6661 gactatgtgt cgtcctacgc cagcatgcat cccgcggagg actgggcgga gacgttcgcc
     6721 cactacctgc acatccgcga cgcgctcgac acggcagcgg cgttcggttt cgcgcaggcc
     6781 gccgcgacgt ttgagcgacg ggcgttgggt cccagcggat ttgacacgat catcgatatg
     6841 tggctgccgc tggcatgggc gctgaacatg gtgaaccgct cgatgggtaa agaggacctt
     6901 tacccgttcg tgcttccccc ggcagtgctc gagaagatgc ggttcatcca caccatcatc
     6961 gacgagatca cctcggaccc cgccaaactc actgcctagc ttgccgcgag cgtgcgtgtc
     7021 tgcacgtcga cacgccgcag agcgcctaca ttttgcgcac gttcgccgcc ggcgagagtg
     7081 cgggaaatgt tcaccaatgt cagcgtttct gtgttcagac acgcacgctc gcgcgagacg
     7141 ttgctcagct ccagtacgcg gcggtcgtgg tgtataaccg cccgactagc tcgtcgacgg
     7201 ataactcgcg cagttgcgcg ctgagcacct caacgctgac agtgccgtcc cagccgcgat
     7261 cgagaaacgt tgcgacgaaa cggtctaact cgagaatccc ttcgccgggc agcgcgcgac
     7321 ggtgcaggct ttcgcggacc atgcggtctc gatactccgg ttcgagcgcg tcggtgaact
     7381 ggacataggc catatcttcg aggggcaccg ccgccaagtc gtcccacgtg tcgtcgctga
     7441 agcagaagtg ccaggagtcg accagcaggc cggcacgcgc ggtgcggctc gcggcccgca
     7501 cgtactccat gccatcgcgg atcgacggaa tcgcacccag cgggctgtac tccaccgcca
     7561 tgcccgcgcc aacaccgtca aa
//
LOCUS       DASRJA010000104        10396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3906728,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000104 DASRJA010000000
VERSION     DASRJA010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10396
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3906728"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VFC01_04750"
     CDS             complement(<1..925)
                     /locus_tag="VFC01_04750"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZN78988.1"
                     /translation="MTTTITPTPKTLQKTVGGKTVAMTPEQAEAFGRELDALKERVIA
                     DLGDRDATYIRRIIKAQRTLEVGGRALLFGSIFPPFWLAGTAMLGLSKIIDNMEIGHN
                     VMHGQYDWMGDPAISSRGFEWDTACPGDQWRHSHNYMHHTYTNIVGMDRDIGYGILRM
                     SEDQKWRPYFLGNPIYAFLLMVLFQYGVALHELETERIRSGEITLADKREILKGIRRK
                     TKRQALKDYVAFPLLAGPFAPLVFAGNLTANLIRNVWSFMIIFCGHFPEDVQEFSIEE
                     TKAETRGMWYFRQILGSANLTGGKLFHILSGN"
     gene            complement(942..2006)
                     /locus_tag="VFC01_04755"
     CDS             complement(942..2006)
                     /locus_tag="VFC01_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HZN78989.1"
                     /translation="MFTQTLTRRVLASPLVDLLTGPHGVDRYTELVAPTWTTGEARAK
                     IVAVRGQTPRSVTLTLEPNRAFTGFRAGQHINLTVEIDGRRRTRPYSPASAEGAPYIE
                     LTIGRHDSGLVSNYLYEHARPGMVVGLDSVGGDFVLPAVRPRRILFVSGGSGITPVMS
                     MLRTLRAEGFTGEVAFIHYARAAEEACYRDELDAMPGVKVLHGFTRTTSGSDLHGRFG
                     PEHLAAAMPDPGEDTVVFVCGPPALVEAVREHCANVRSESFVPPVFDLPAEASGGRVS
                     FTDSGTEIVDDGRPLLEQAEAAGLMPESGCRMGICHSCTRRKTRGAVKNLITGAVSSA
                     EQEDVQICVSVPVGDVDIAL"
     gene            2076..2759
                     /locus_tag="VFC01_04760"
     CDS             2076..2759
                     /locus_tag="VFC01_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN78990.1"
                     /translation="MNSRTPSSRTSRSGRSGKSRSRDTLSREERKEATRRAIVAAALK
                     LLEDRSFAALSLREVTREAGIVPAAFYRHFESMEALGLVLIDESFRRLRDMLRTARAG
                     KLDPNRVIESSVDILIDGVNEDREHWRFIGRERSSGVTVLRYAIRTEIRLIVSELAID
                     LARFPGLNTWSGEDLNILASLFVNSMIVIAEAIEDANDTAAIEEIKRIAVKQLRMIAV
                     GVSGWRSAI"
     gene            2770..3549
                     /locus_tag="VFC01_04765"
     CDS             2770..3549
                     /locus_tag="VFC01_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN78991.1"
                     /translation="MPHLELTYPRPDIAVLTLNRPEKLNALDYELVEDLHAALDGIRA
                     NNDCRVVVLTGAGRGFCSGLDLSDPNPSQAGGGTEFPRSGMRWQELIADLTAKIHRLR
                     QPVIAAVNGPAYGGGFGIALACDIRIASESARFCTQFIKLGIGGCDIGVSYTLPRIIG
                     AGPAFDLILTARVVDAAEALRLGLVSRLSDSVVDEAMAIAETLCGYGKFGVESTKQVM
                     WANVDATSLESALHLENRSQILAGAAGVMGEATEAFRHRQR"
     gene            complement(3518..4288)
                     /locus_tag="VFC01_04770"
     CDS             complement(3518..4288)
                     /locus_tag="VFC01_04770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN78992.1"
                     /translation="MDREAFDRLFDMTGRSVIVTGGTRGIGLALAEGFVLAGARVVVA
                     SRKPDACEQAAEHLRGLGGEAIGVPTHLGDVDSLGVLVQRTVDEFGGIDVVVNNAANA
                     LAQPLGQMTVDAWTKSNEVNLRGPVFLVQEALPHLKESQHAAVLNMISTGAFQFAPVV
                     SMYAAGKAALLSFTRSMAAAYVPFGIRVNALAPGPVDTDMMRNNPPEAIEYMKGGTLM
                     KRLASPDEMVGAALLLTSDAGSYITGQVIIADGGGTPR"
     gene            complement(4294..5193)
                     /locus_tag="VFC01_04775"
     CDS             complement(4294..5193)
                     /locus_tag="VFC01_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZN78993.1"
                     /translation="MYLITGAGGGIGSVSRRVVQLLLDGGAQVRAMVRRDDDRADQLR
                     EMGADVIVGDLTSAGDIVNAVDGVRRMFFNMSVSPDYLRATTEVCAVALDRGQLDVIV
                     NMSQMTVSQMTLTSTDESKQHRLHWLAEHVLNWSGLPVIHVRPTTFLDNPLFTFLAKQ
                     AVRDRGVLTLPFGAGRTSPISADDVARVVAALLRDPADRIGQIYELTGPEVLDINGLA
                     EQYSRALNRPISAEDVPLDEWERQVLEPVGLPDHVRQHIATMARLHREGRYDRATGDV
                     EQITGQPAQTVEQYVASNPDLFY"
     gene            complement(5198..5581)
                     /locus_tag="VFC01_04780"
     CDS             complement(5198..5581)
                     /locus_tag="VFC01_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HZN78994.1"
                     /translation="MSASLEPTPDQIAALAARPADEPVVMINLLQFRADGGRQSYIRY
                     IQEVTPHLQRVGGTVRYAGESPTVVIGDGEKPWWDAIIVVEYPSPAAFLNMVTDEDYL
                     KVHEHRAAGLDRGDLVATSIWTRAE"
     gene            complement(5584..5832)
                     /locus_tag="VFC01_04785"
     CDS             complement(5584..5832)
                     /locus_tag="VFC01_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZN78995.1"
                     /translation="MRKIWLFAAAVIFAAGLMIPTAPAARATVCGSIGGARVDVSGCA
                     DPFYELNDALTAPPPPPPAPNVSVCVNAGRRISVSGCI"
     gene            6481..8364
                     /locus_tag="VFC01_04790"
     CDS             6481..8364
                     /locus_tag="VFC01_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HZN78996.1"
                     /translation="MMVNAPANAVSAWHPKTPPVSTPWTDLVGPDNALPQYPRPQMAR
                     TSWMNLNGLWNYTGRSGQDTLTAPPPATEYRERILVPYPPESALSGIQRHDDQMWYRT
                     VFEVPSSWGGRRVLLHFGAVDQIATVWVNNQQVAYHEGGYTEFSADITGTLKPGLQQL
                     TVRVEDRNEANPFPVGKQRNRPEGLFYTGSSGIWQTVWLEPVRPTHIDRLDIAPDLTG
                     FTITPKVSGTQYQRVQVVVSKPGGAEAMVRSGQPGATLRIPVLNPRLWTPDDPFLYDI
                     KVRLVGRSGKVVDEVSSYAGLRTIGTVPDAKGRPRIALNGKITFLHGPLDQGYWPDGI
                     YTAPTDDALRFDLEQIKALGMNFVRKHAKVEPARWYYWADKLGLLVWQDMPSLDVSLD
                     IPVGPAPDPVPPAKANFEKELSAIIDQLRSVTSIVGWVPFNEGWGEFDTARIANAVKA
                     QDPTRMVDANSGVNCCKSRGDSRAGDVYDDHTYVGPGRPVLHDNPTTPQDERLMLDNH
                     PVPLEHRVVVDGEYGGLGLVLDKNRWPGLPQAYEMTDSQERLTQRYVEVSQDLEKAVQ
                     EGGLSGAIYTQTTDVENEVNGLLSYDRQVVKMALPVVAERNRAVIVAGAQATDPPAPP
                     DRR"
     gene            9137..>10396
                     /locus_tag="VFC01_04795"
     CDS             9137..>10396
                     /locus_tag="VFC01_04795"
                     /inference="COORDINATES: protein motif:HMM:NF036814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZN78997.1"
                     /translation="MAAGSRRNVGRSKSRHRRSRRAGGAQAVYPTFRIYPSPTGDMPG
                     AGTSTGYAKHVGRVGALALALGVGMAVATGQGMGFARADDSPSVDSNSVPSGPSPTGE
                     SGDLNSPTPPSNTPTPTSSSSEPPSAPTNTPSVPEMNYDSSGGALTSGDEDKKTPPKT
                     DPKSVPTVSSSLTAPTPTPQPKNNPQQGNSTPAAHPSATVAADSSEPPSSGDAPKPAE
                     PTTTVTVLGEHTQARLVPGPNGGEIGATDSQQSPTASAMEVSALSAPQESTASPGIVD
                     LTTTVLAAALAPFVQPGPGGAPSDAPVLLAVLAWARRETQRALGSGKPEVTPQQTTSA
                     VDEPVATTEAIALSAKTTKAPAAKKGGGGGNTKPVATDDSYTTNEDNTLTIGPATGVL
                     ANDTDADGNSLSATLVSGPKNGTVNLNP"
BASE COUNT         1834 a         3330 c         3436 g         1796 t
ORIGIN      
        1 ggttgccgct caggatgtga aagagcttgc cgccggtcag gtttgccgat ccgaggatct
       61 ggcgaaagta ccacatcccc cgcgtctcgg ccttggtctc ctcgatggag aactcctgaa
      121 cgtcctcggg gaagtggcca cagaagatga tcatgaacga ccagacgttg cggatcaggt
      181 tggcggtcag gttgcctgcg aacaccaacg gcgcgaacgg tcctgccagc agcggaaacg
      241 ccacgtagtc cttgagcgcc tgccgcttgg tcttgcgccg gatgcccttg aggatctcgc
      301 gcttgtccgc cagcgtgatc tcgccggagc ggatgcgctc ggtctcgagc tcatgcagcg
      361 cgacgccgta ctggaacagc accatcagca ggaacgcata gatgggattg ccgaggaagt
      421 aggggcgcca cttctggtct tcgctcatcc gcaggatgcc gtacccgatg tcgcggtcca
      481 tcccgacgat gttcgtgtac gtgtggtgca tgtagttgtg cgagtggcgc cactggtcgc
      541 cggggcacgc ggtgtcccat tcgaacccgc ggctggagat cgccggatcg cccatccagt
      601 cgtactggcc gtgcatcacg ttgtggccga tctccatgtt gtcgatgatc ttcgacaggc
      661 cgagcatcgc ggtgccggcc agccagaacg gcgggaagat gctgccgaat agaagcgccc
      721 gtccgccgac ctctaaggtg cgctgcgcct tgatgattcg ccggatataa gtggcgtcac
      781 gatcgccgag atcggcaatc acgcgttcct tcagtgcgtc gagctcgcgg ccgaacgcct
      841 cggcctgctc gggggtcatg gcgacggttt tcccgccgac cgtcttctgc agtgtcttcg
      901 gggtgggagt gatcgtggtg gtcatgtcga gcacctttcg gtcagagtgc gatgtcgacg
      961 tcgccgacag ggacggacac gcagatctgc acgtcctcct gctcggcgct cgacaccgct
     1021 ccggtgatca ggtttttcac cgcgccgcgg gtcttgcggc gcgtgcagct gtggcagatg
     1081 cccatccggc atccgctttc gggcatcagc cccgcggcct cggcctgctc cagcagcggc
     1141 cggccgtcgt cgacgatctc agtgccactg tctgtgaatg acactcggcc gccggacgcc
     1201 tcggccggga gatcgaacac cggcggaacg aagctctccg accgcacgtt cgcgcaatgc
     1261 tcgcggacgg cctcgaccaa agccggtggg ccgcagacaa acaccactgt gtcttctccg
     1321 gggtcgggca tcgcggcagc cagatgttcg ggaccgaacc gaccgtggag atcagagccc
     1381 gaggtcgtac gcgtgaagcc gtgcaacacc ttgacgccgg gcatggcgtc gagttcgtcg
     1441 cggtagcagg cctcctcggc ggcgcgcgcg tagtggatga acgcgacctc gccggtgaag
     1501 ccctcggcgc gcagtgtgcg caacatcgac atcacaggcg tgataccgct gccgccagac
     1561 acgaacaaga tgcgccgagg ccgcactgcg ggaaggacga agtcgccgcc gacagaatca
     1621 agcccaacga ccatgccggg gcgggcatgc tcgtagaggt agttggagac caggccgctg
     1681 tcgtggcggc cgatcgtgag ttcgatgtac ggggcgcctt cggcgctggc cggtgagtag
     1741 ggccgggtgc ggcgacggcc gtcgatctcg accgtgaggt tgatgtgctg gcccgcgcga
     1801 aagccggtga acgcccggtt gggctccagg gtcagtgtga cgctgcgcgg ggtctgcccc
     1861 cgcaccgcga cgatcttggc ccgggcttca cccgtggtcc aagtcggggc gaccagctcg
     1921 gtgtaccggt ccacgccatg cggcccggtc agcaggtcga ccagcggtga tgccagcacc
     1981 cgccgcgtca aagtttgagt gaacatgcgt acaccgtgcg gcatgacggg gccgggcgtc
     2041 aaccgattcg agcaggttgt ggtaggtttc acacagtgaa cagtcgtacg cccagttcac
     2101 gaacgagccg atctggtcgt tcgggcaagt cgcgctcccg cgacacgctg tcgcgcgagg
     2161 agcgcaaaga agccacccgg cgggccatcg tcgcggcggc gctcaagctg ctcgaggatc
     2221 gcagtttcgc cgcgctgagc ctgcgtgagg tcacccggga ggcgggcatc gtgcccgcgg
     2281 cgttctatcg gcatttcgag tcgatggagg cgctcgggct ggtgctgatc gacgagtcgt
     2341 tccgccggct gcgcgacatg ctgcgcaccg cgcgggccgg caagctggac ccgaaccgcg
     2401 tcatcgaatc ctcggtcgac atcctcatcg acggcgtcaa cgaggatcgc gaacactggc
     2461 gattcatcgg acgcgagcgg tccagcggcg tgacggtgtt gcgctatgcg atccgcaccg
     2521 aaatccgctt gatcgtttcg gagttggcga tcgacctggc ccgctttccc ggtctgaaca
     2581 cctggagcgg tgaggatctc aacattctgg cgagtctgtt cgtgaactcg atgatcgtga
     2641 tcgccgaggc catcgaggac gccaacgaca cagcggcgat cgaggaaatc aagcgcatcg
     2701 cggtcaagca actgcggatg atcgccgtcg gcgtcagcgg gtggcgcagc gccatctagc
     2761 gtgaggtgca tgccgcacct cgaattgacc tacccgcgcc ccgacatcgc cgtgctcacc
     2821 ctgaaccggc ccgagaagct caacgcgctg gactacgagc tcgtcgagga ccttcatgcc
     2881 gccctcgacg ggatccgcgc gaataacgac tgccgggtcg tggtgctcac cggcgctggg
     2941 cgcggcttct gctcgggcct ggatctgagc gatccgaatc cgtcgcaggc gggcggcggc
     3001 accgagttcc cgcggtccgg catgcgctgg caagagctca tcgcggacct caccgcgaaa
     3061 atccatcgtc tgcggcagcc ggtcattgcc gcggtcaacg ggcccgcgta cggcggtggc
     3121 ttcggcatcg cgctggcctg cgacatccgt attgcttccg agtcggcgcg gttctgcacc
     3181 cagttcatca agctgggcat cggcggctgc gacatcggcg tcagctacac cctgccgcgc
     3241 atcatcggtg ccgggccggc gttcgatctg atcctgacgg cgcgtgtggt cgatgccgcc
     3301 gaggcgctgc ggctgggcct cgtctcgcgg ttgtcggact cggtcgtcga cgaggcaatg
     3361 gccatcgccg aaacactgtg tggctacggc aaattcgggg tggagtccac caagcaggtg
     3421 atgtgggcga atgtggatgc gacgagcctg gagtccgcgc tgcatctgga gaaccgcagc
     3481 cagattctcg ccggcgcagc cggcgttatg ggcgaagcta ccgaggcgtt ccgccaccgt
     3541 cagcgatgat gacctgcccg gtgatgtagc taccggcatc cgacgtcaaa agcaatgcgg
     3601 cgccgaccat ttcgtcgggc gaggccagcc gcttcatcag cgtgccaccc ttcatgtact
     3661 cgatcgcctc cggcgggttg tttcgcatca tgtcggtgtc gacgggtccg ggcgctagcg
     3721 cgttgacccg gatgccgaac gggacgtagg ccgcggccat cgaccgcgtg aacgacaaga
     3781 gtgccgcctt gccagccgca tacatcgaga ccacgggcgc gaactgaaag gcgcccgtcg
     3841 agatcatgtt cagcaccgca gcgtgctgac tctccttgag atgcggcagc gcctcctgca
     3901 ccaggaacac aggtccacgc aggttcacct cgttcgactt ggtccaggcg tcgacggtca
     3961 tctgtcccag cggctgcgcc aacgcgttgg cggcgttgtt caccactacg tcgatgccgc
     4021 cgaattcgtc gaccgtccgt tggacgagga cgccgaggga gtcgacatca ccgaggtgcg
     4081 tgggcacgcc gatggcctcg cccccgagcc cgcgcaggtg ctcggcggcc tgctcgcagg
     4141 cgtccggttt gcggctggcg accacgacgc gggcgccggc gagcacgaag ccctccgcca
     4201 acgccaggcc gatgcctctg gtgccccccg tcacgatgac agagcggccg gtcatgtcga
     4261 agagacggtc gaaggcttcg cgatccacgg cgctcagtag aacagatcgg gattcgacgc
     4321 cacgtactgc tcgaccgttt gagcgggctg accggtgatc tgctcgacgt cgccggtggc
     4381 gcggtcgtag cggccttcgc gatgcaaccg cgccatggtg gcgatgtgtt gtcggacgtg
     4441 gtcgggcagg cccaccggtt cgagcacttg gcgctcccat tcgtcgaggg gaacgtcttc
     4501 agccgagatc ggccggttca gcgctcggct gtactgctcg gcgaggccgt tgatgtcgag
     4561 cacctccggt ccggtcagtt cgtatatctg gccgatccgg tcggcggggt cgcgcagcaa
     4621 ggcggccacg acgcgggcga cgtcgtctgc ggagatcggc gaggtgcggc cggcgccgaa
     4681 cgggagggtc agcacgccgc ggtcgcggac agcttgtttc gccagaaagg tgaataacgg
     4741 gttgtcgagg aaagtggttg gcctgacgtg gattaccggt agacccgacc aattgagcac
     4801 gtgctcggcc agccagtgca ggcggtgttg tttcgactcg tcggtgcttg tcagcgtcat
     4861 ctgcgacacc gtcatctggg acatgttgac gatcacgtcg agctggcctc ggtcgagcgc
     4921 cacggcgcag acctcggtgg tagccctcag gtaatccggt gacacgctca tgttgaagaa
     4981 catgcggcgc acaccgtcga ccgcgttgac gatgtcgcct gcacttgtga ggtcgccgac
     5041 gatcacatcg gcgcccatct cccgcagttg gtcggcgcgg tcgtcgtcgc gccgcaccat
     5101 ggctcgcacc tgtgcgccgc cgtcgagcaa gagctgcacg acccggcggc tgacgctgcc
     5161 gatgccgcct ccggcacctg tgatcaggta cacggcgcta ctcggcgcgg gtccagatcg
     5221 aggtggcgac gaggtcgccg cggtccagcc ccgccgcgcg atgttcgtgc accttgaggt
     5281 agtcctcgtc ggtcaccatg tttaggaacg cagcgggcga cgggtattcg accacgataa
     5341 tggcgtccca ccatggcttt tcgccgtcgc cgatcacgac cgtcggcgat tcgcccgcgt
     5401 agcggacggt gccgccgacc cgctgcaggt gcggcgtgac ctcttgtatg tagcggatgt
     5461 agctctgcct gccgccgtcg gcgcggaact gcagcaggtt gatcatcacg acgggttcgt
     5521 cggccggccg tgcggcgagc gcggcgatct gatcgggagt cggttccagg gatgcactca
     5581 tcgctagatg cacccgctca cgctgatccg ccggcccgca ttcacgcaga cactcacgtt
     5641 gggagccggg ggcggaggcg gcggcgctgt cagggcatcg ttgagttcgt agaacggatc
     5701 ggcgcatccg ctgacatcca cgcgtgcgcc gccgatcgag ccgcacacgg tggcccgtgc
     5761 cgcaggcgcg gttggaatca tcaagcccgc ggcgaagatt accgccgcgg cgaacagcca
     5821 aatcttgcgc acgttgtgct ccttcgccta atcaggctca tcctgcaccg tcgttcacgg
     5881 agcactggtg gtttcaggat tccgatgcgg caggccagca cgaactggca gagccctgcg
     5941 aggctacctt ttgctcccta gggaagcatc gctcgcctgg aacggcgccg acgatccggc
     6001 acgaccgaca caatacctgt tggcttcgca cggtttatgg ccttgcaact tgacaatctc
     6061 cgttatcact gtgcgccgaa agttactgtg cgaaaagaat ttcggatttg gtagcgatct
     6121 tgattgacgc taggctacat ttgccgtcgg caacccatcc tcccgacctg ttcggcgcgc
     6181 gtcgtaggga actgctggac ttcctcgtcg ccagctgggt ggtttcgttt gctgtacagc
     6241 aacggtgcgc tggggtcagg ttgggcacgc gccgcacgtc ggtcttgtcc gtgttcaacc
     6301 ttgggggtag ctactgtggc cgctggcttc gagccgtggt atcgccgcga agtccagcag
     6361 gaacgatcac tcattcggcc ctatcgctca cgcggtgacc gccgctggtg cgtcgtgggg
     6421 ttgaatccct tgcggatcag caggttcgcg gccgtggggt tcgcgctgtt aggtgtcagc
     6481 atgatggtta acgcgccggc taacgccgtg tcagcctggc atccgaagac accacccgtg
     6541 tcaacgccgt ggactgacct ggtgggacct gacaacgcgc tgcctcagta cccgcgtccg
     6601 cagatggcgc ggacgagttg gatgaacctc aacggcctgt ggaattacac cggccggtct
     6661 ggtcaggaca cgttgacggc accgccgccc gcgaccgagt acagggagcg gatcctggtg
     6721 ccttatccgc cggagtcggc gctgtccggt atccaacgcc acgacgacca aatgtggtac
     6781 cgcacggtct tcgaggtgcc gagtagttgg ggcgggcggc gcgtgctgct gcacttcggc
     6841 gcggtcgacc agatcgccac cgtatgggtg aacaaccagc aggtggcgta tcacgaaggg
     6901 ggatatacgg agttcagcgc cgacatcacc ggaacgctca agccaggttt gcagcagttg
     6961 accgtgcgag tggaggaccg caacgaggcc aacccgttcc ccgttggtaa gcagcgcaac
     7021 aggccggaag ggctgttcta taccggatcg tcaggcatct ggcagacggt atggctggaa
     7081 ccggtccgcc ccacgcacat cgacagactt gacatcgcgc cggacttgac cggtttcaca
     7141 atcacgccaa aggtttccgg aacgcaatac cagcgcgtcc aggtggtcgt ctccaagcca
     7201 ggtggcgcgg aagcgatggt gcgctcgggc cagccgggag cgacgctgcg gatccccgtg
     7261 ctgaacccgc gactgtggac gccagatgac ccgttcctct acgacatcaa ggttcgcctt
     7321 gtcggccgat ccggaaaggt ggtcgacgag gtctcgagct acgccggctt gcgcaccatc
     7381 ggcaccgtgc cggatgccaa gggccggccg cggatcgcgt tgaacggcaa gatcactttt
     7441 ctacatggtc cgctggatca ggggtactgg cccgacggga tctacaccgc acccaccgac
     7501 gacgcgttga ggttcgacct cgagcagata aaggcactgg ggatgaactt cgtccgcaag
     7561 cacgccaagg tggaaccggc gcggtggtac tactgggccg acaaactcgg cctgttggtg
     7621 tggcaggaca tgccttcgct cgacgtgtcg ctcgacattc cggtgggtcc cgcgccggat
     7681 cccgttccgc cggcgaaggc gaacttcgag aaggaactat cggcgatcat cgaccagctc
     7741 cgcagtgtga cctcgatcgt cggctgggtg cccttcaacg agggttgggg cgagttcgac
     7801 accgccagaa tcgcgaacgc ggtgaaagcc caggacccga ctcggatggt ggacgcgaac
     7861 agcggcgtca actgctgcaa atcgcgcggc gacagcaggg ccggagacgt ctacgacgat
     7921 cacacctatg tcggtcccgg ccgtccggtg ctgcacgaca acccgacgac tccgcaggac
     7981 gaacggctga tgctcgacaa ccatccggtg ccactcgagc accgcgtggt ggtcgatggg
     8041 gagtacggcg gcctcgggct ggtgctcgac aagaaccggt ggccggggct gccgcaggcg
     8101 tacgagatga ccgacagcca ggagcggctg acacagcggt atgtcgaggt cagccaggat
     8161 ctcgaaaagg cggtgcagga aggcggtttg tcgggtgcga tctataccca gacgacagac
     8221 gtcgaaaacg aggtcaacgg attactgagc tatgaccggc aggtggtcaa gatggcgctt
     8281 ccggtggtgg cggaacgaaa tcgcgcggtg atcgtggcag gggcacaagc aacagacccg
     8341 cccgcgccgc ccgaccgtcg ttgatgacgg ccgtctggtg atgaactcgc gcagatgatg
     8401 tctactaatc ggatcgcaga actcggagct tgggtcgaga atttgcaagc gtcgcgagaa
     8461 attgtcgggc gcaacgtcaa cgacgttgcg gtacaagcga tcatgacgat cggcgcgccc
     8521 agcgaacacg tgtgcagccg cagccggtcc ttcttcggcg cggccgcgct gggccgggcg
     8581 gcaactcaca gccgtcgtcc gaccgtcgga gctcaccatg cggtgcgtgc gcgtccaacg
     8641 atttgccagc tgcagccgac acgctggctg gatgctgttc gcggaatcag agccggggca
     8701 cacgtccgtt agccatatgg aacacgcatc tgggtcagca atccacatac aactgagctt
     8761 gaccgctgcg tcgctctcgc gatgcggagg tgacagatcc gctcgactca ccgatagatg
     8821 agttcttgcg attatgcgta gaaccgatcc gcgatcggcc gggttttgcc tgtggggcgg
     8881 acctacctta tcagcttcgc cagaacgttc tcgccaaccg aatcagcgat ggcaattttg
     8941 tgcgttcctt agcgaatcga ttttcatcgg cgtaaatgtt gctgatgcgc agaagcaccg
     9001 aaggtaggga aacacccgat tcctcatcgt cgggctgacc gtaaatttgc tgttatcgca
     9061 gcgccgccgg gaccggacgt gcgctgctgc gttaggaaat ccttaccggg gcccgaagca
     9121 gtggggggta gcaaccatgg ccgctggatc gcgacgcaac gtcggccgca gcaagagtcg
     9181 ccatcgcagg tcaaggcggg ccggtggggc gcaagccgtc tatccgacgt tccgcattta
     9241 tccgtcgccg accggcgaca tgcctggtgc aggcacctcc accggctatg ccaagcatgt
     9301 cggtcgggtc ggcgcgctgg ccctcgcact cggcgtcggg atggcggtcg ccactggcca
     9361 gggaatgggt ttcgcacgcg ccgatgattc gccgtcggtt gattcaaatt cggtgccgtc
     9421 gggtccttcg ccgacgggtg agtcaggtga tctgaactcg ccgacgccac cgtcgaatac
     9481 gccgacgcca accagctctt ccagcgagcc gccgtcggcg ccgaccaaca ccccgtcggt
     9541 ccccgaaatg aactacgaca gctccggcgg agcactcacc tccggcgatg aagacaagaa
     9601 aactccaccg aaaacggacc cgaagtccgt gcccaccgtt tcctcatccc tcacggcgcc
     9661 gactccaacg ccccaaccga aaaacaaccc gcagcaaggc aattcgacgc cagccgccca
     9721 tccttccgcc accgtggcgg cggattcctc cgaacccccc tcgtcaggcg acgcacccaa
     9781 gccggcggag ccgaccacaa cagtgacggt ccttggcgag cacacccagg cccgcctggt
     9841 ccccggtccc aacggtggcg agatcggggc gaccgattca cagcagtccc ctactgccag
     9901 cgcgatggag gtgtctgcgc tcagtgcgcc acaagaatcg accgcatcgc cgggcatcgt
     9961 cgatctgacg accacggtgc tggccgcagc gttggcgccg ttcgtgcagc cgggcccggg
    10021 cggcgccccg tccgacgcgc cggtgctgtt ggccgtgctg gcctgggcac gccgcgaaac
    10081 gcagcgcgcc ctgggcagcg ggaaaccgga ggtaacgcct caacagacca cgtcggcagt
    10141 cgacgagccg gtcgccacca ccgaagcgat cgcgttgagc gccaagacga ctaaggcccc
    10201 cgccgcgaag aagggcgggg gcggcggcaa caccaaaccg gtggccaccg acgacagcta
    10261 caccactaac gaggacaaca cgctgaccat cgggccggcc accggggtgc tggccaacga
    10321 caccgacgcc gacggcaact cgctgagcgc cacgttggtg tcgggtccga agaacggcac
    10381 tgtgaacctc aacccc
//
LOCUS       DASRJA010000105         5981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4228669,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000105 DASRJA010000000
VERSION     DASRJA010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4228669"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFC01_04800"
     CDS             <1..292
                     /locus_tag="VFC01_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HZN78998.1"
                     /translation="KAGVFFDASAQRWPVCRQYTHTQSESQWSVGQISNANVTLSTTA
                     TQQNASAPGWACGRALALRNNVMIDINTCSANPADSASKIANQIAANVTARW"
     gene            925..1119
                     /locus_tag="VFC01_04805"
     CDS             925..1119
                     /locus_tag="VFC01_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZN78999.1"
                     /translation="MSGADKARNKLQQVKGKIKAATGRATDDPGLEARGRADQRTSHL
                     KDAGEKVKDAFRPRRRRRQM"
     gene            1137..1958
                     /locus_tag="VFC01_04810"
     CDS             1137..1958
                     /locus_tag="VFC01_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HZN79000.1"
                     /translation="MTGGTSPTGVVRDATADPVARVAARAGYVISGVLHLLIAYIIFR
                     IAIGSGGDADQTGALRAIAGTTGGNIALWAVAVALVPLTLWRLAETVLGLHPGERHDR
                     DPKDFRLANRLKALGLAVVYAAVTVTAVQFALGGRKSSAAQNAGLSGRLMRSMEGKAV
                     LVVIGLAITAIGCYFAYKGASRRFCNDLTVSPGRAITMLGLFGYVAEGLVLAGAGILV
                     IVAAVRADPSKATGLDGAVKTLGQAPHGTVLIMCAAAGFAAYGCYSFALSRYARM"
     gene            2032..2235
                     /locus_tag="VFC01_04815"
     CDS             2032..2235
                     /locus_tag="VFC01_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79001.1"
                     /translation="MSLLDMRFQQSCPHMIPVGPLRPLGHARVLGCHSATVEMSVAYS
                     VPEAYERMMRLVTAEGEQPRWFM"
     gene            2495..2986
                     /locus_tag="VFC01_04820"
     CDS             2495..2986
                     /locus_tag="VFC01_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79002.1"
                     /translation="MGDTVNGDRCYATVLIDEELWRSLATLDLAQEVAWVHGCVLEHG
                     HRGDHHALAHHAGPLSYWLQWDETRRPRISTTDRPDPLAREASPRIEPSGQPQHAERP
                     TAAKHSSTAAAEPVGSKNTGSQAEALWAIAAALERLADVIVGALDPAESTGRHAAGHD
                     ERW"
     gene            complement(3615..4163)
                     /locus_tag="VFC01_04825"
     CDS             complement(3615..4163)
                     /locus_tag="VFC01_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZN79003.1"
                     /translation="MATESTMLALGTPAPRFALPEPATGATVSLDELTGPALVVTFIC
                     NHCPYVQHVAAGLAALGRDLTDRGVAMVGISSNDVVTYPQDGPDQMVAEARRHGWTFP
                     YLYDETQDVARAFSAACTPDTFVFDGERRLVYRGQLDDSRPRKDLPVTAADVRAAVDA
                     VLAGRPVEPDQRPSIGCGIKWR"
     gene            4247..4636
                     /locus_tag="VFC01_04830"
     CDS             4247..4636
                     /locus_tag="VFC01_04830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005562704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZN79004.1"
                     /translation="MTGHIPLNPQQLDAEKVVLLQTRKRDGSWVDTPVNIAVRGDRAY
                     FRTPGKASKNKRLRNFPEVQIRPCTWSGKPTGGTTMTAHARLLSGEESEAAGRLIDRK
                     YPVLQRLLVRLTHKIMRTPTLHYELAP"
     gene            complement(4823..5794)
                     /locus_tag="VFC01_04835"
     CDS             complement(4823..5794)
                     /locus_tag="VFC01_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN79005.1"
                     /translation="MDSVRPCPAYVGLGVDEISRRAAQARVLLGPDRCRVCPRLCRVD
                     RLSDVKGLCRIGRTAVVASHFAHFGEENCLRGRNGSGTIFFSGCNLRCVFCQNYDISW
                     ELCGEQVTPQRLAQMMLELQDKGCHNINFVTPEHVVPQILEALPHAIDAGLRLPIVYN
                     TSAYDSLDSLVLMDGIVDIYMPDAKVWSRERARRFLRMPDYPDVMRATIPEMGRQVGK
                     LVLDHDGLAVRGLLVRHLVMPGMFDETAEILRWIAGMLGPDTYVDLMAQYFPAGLVGR
                     TDVRDPYPEINRHLHRDEYVRAAELADELGLRRLDRRSVASGYLLAT"
BASE COUNT         1147 a         1898 c         1911 g         1025 t
ORIGIN      
        1 aaaggccggt gtgttcttcg atgcctccgc ccagcggtgg ccggtttgcc gccagtacac
       61 ccacacgcag agcgagtcgc agtggtccgt cgggcagatc tccaacgcta acgtcacact
      121 gagtaccacc gcgactcaac agaacgccag tgccccaggc tgggcctgtg gacgcgcgct
      181 agcgctcaga aacaatgtca tgatcgacat caacacgtgc agcgctaacc ccgctgactc
      241 ggcgtcgaag attgccaacc agatcgctgc caatgtaaca gcgcggtggt agcgctgccg
      301 aactagccga caggcactgc ttggacgttg agccgatcca accctcgcgc ggctccccgc
      361 gacaccgcta gagtgcacgg ttgctcaggt tctcccccaa cacctatgcg gtggcgaata
      421 cggcgggatt gaatacggcg taccggtacc cactgcatat tccagcagat gaatcaggga
      481 cgcggaggtc tcgaagccct gctagacgcg ggagctcctc tgtggcacaa cgctgttggg
      541 aagatcgtgc ataccgtacg gataaccagc taacgactct gcaaccgcag aactaagggg
      601 ccagttcgac ggcggaatgg actgcaacgt aagccttttc gacacgacca tctcgatgct
      661 gacctatccc gccatctgga atctcaacgg cgaattcgag ccgaagcgta caacaatcac
      721 ccccagtgca gtcgtcgaaa cccaagcgga ttcggcggcg gagccgctaa cacgagcgac
      781 accggtattc gacaccactg ccggtggccg cccgattggg atatcgcggg ccgaaggatc
      841 aaagggagcg aggtgtcggt gacgaaaatg ggtaaccgct tggtggtgct tcactttgga
      901 ggcagagagt tagaaggatt tgctatgagc ggtgcggata aagccaggaa caaactgcag
      961 caggtcaagg gcaagatcaa ggcggcaacc gggcgcgcga ccgacgaccc cggcttggaa
     1021 gctcgaggca gggccgatca gcggacatct cacctgaaag acgcgggcga aaaagtcaaa
     1081 gacgcattcc gcccccggcg ccgccgccgc cagatgtagc ggagactcga tccctcatga
     1141 ctggcgggac cagtccgacg ggtgtcgtcc gcgacgccac cgctgacccg gttgcccgcg
     1201 tcgcggctcg cgccggctat gtcatcagcg gggtcctgca tctgctgatc gcctacatca
     1261 ttttccgaat cgcgatcgga tccggcggcg acgcggatca gaccggcgct ttgcgagcca
     1321 tcgctggtac taccgggggc aatattgcat tgtgggccgt tgcggtcgca ctcgtcccgc
     1381 tgacactatg gcgcctagcc gagacggtcc tgggcttgca tccgggcgag cgccacgacc
     1441 gcgaccctaa agattttcga ctggcgaacc ggctgaaggc gcttggtctc gcggtggtct
     1501 acgccgcagt caccgtcacc gcagtgcaat tcgcgctcgg cggccgcaaa tccagcgccg
     1561 cccagaatgc cgggctcagc ggccggttaa tgcgatccat ggaaggtaag gccgtcctcg
     1621 tggtgatcgg gctcgctatc accgccattg ggtgctactt cgcctataag ggcgcatcac
     1681 gcagattctg caacgacctc acggtttcgc ccggacgcgc gatcacaatg ctgggtttgt
     1741 tcggctatgt cgctgaaggt ctggtgttag ccggtgccgg catattggtc atcgtcgcgg
     1801 cggtgcgcgc tgacccgtcg aaagccactg ggctcgacgg ggcggtcaag accctgggac
     1861 aggcgccgca cggcaccgta ttgatcatgt gcgcggcggc gggttttgcc gcctacgggt
     1921 gctacagctt tgctttgagc cgttacgcgc ggatgtgact tccaccttcc acatcaccgc
     1981 cttcgcacac cagatctgcc cggccgtcgc gttcatccaa ccctctaccc aatgtcgtta
     2041 ctcgacatgc gttttcaaca gagctgcccg cacatgatcc ctgtgggccc gttgcgtcca
     2101 ctggggcacg cgcgggtcct gggctgccat tcggcgacag tcgaaatgag cgtggcctat
     2161 tccgttcccg aggcctacga gcggatgatg cggctggtca ccgccgaagg cgagcaaccg
     2221 cggtggttca tgtgacgccg cgccggggtc ctactcgtcg cttcacgcgt gacctcgcca
     2281 ccaatcgatc gtcttgccag gttaaccgcg ggaagaagca ccccgcgtct cccgcgatcg
     2341 ttgagctcgc ggccggacgg cgtcattttg ttaccgcacc ggtccgcaac aaggtgcccc
     2401 aagcggcgga tactgcatct gaaccgcggc tcacaatcaa gaggggctcg atgacgcacg
     2461 tacacccgct gcgggccgca gtcactttcg gcccatgggc gacacagtga acggcgatcg
     2521 ctgttatgcc acggtgctga tcgatgagga actgtggcgg tcgctggcca cgctggacct
     2581 cgctcaggaa gtggcttggg tgcacggatg cgtactcgaa cacggtcacc gcggcgacca
     2641 ccatgccctg gctcaccacg ccggcccgct tagttactgg cttcaatggg acgaaacccg
     2701 cagaccacgt atcagcacaa ctgaccggcc cgacccactc gcgcgggagg ccagcccacg
     2761 gattgaaccg tccggccagc cacaacatgc cgaacggccg accgcggcca agcactcttc
     2821 gactgcggcc gctgaacccg ttggttcaaa gaacacggga tcccaagccg aagcgctgtg
     2881 ggcgattgcc gctgcactgg aacggctagc cgacgtgatt gtcggcgcgc tcgatcccgc
     2941 ggaatctacc ggccgtcatg ccgctggcca cgacgagcgt tggtgaagaa caacatgcac
     3001 gaggccgtac gagcgccagc gctcggtgat cacctgtgtt ggtgtagaac ctgccgcaag
     3061 caggcaatca aaggttacgt tgctatggtt ctaaacacac cgagctacgg ttctgatagg
     3121 cagccttggc acaccgccca cgcctgtgcg ccgccgccgc taggcgctgg ttgcctctgc
     3181 gcccaggcct ggtcctgaac agtcgacctg tacatgagcg acttcaagac gaacgataac
     3241 ccatcttatc gaccgtgctg ggtgagtcga aaggcattgc tgtacaagat gataacggat
     3301 acggcgtagt ttaccggtaa gcccgccgta ttcgatctcg ccgtaatcgt cgtgcggacg
     3361 ccgattattt agcgatgccg acataggtgg agctgctgcg tcggatggac cgcctactct
     3421 ggggccgcgg gtgcccagag ggcatgcggc agcaagggag ctgtttgctg cgccgggtgc
     3481 ttcttcggta cgcggatggc agtcgcaaat cagcttcgcg agcctcagcc aacagaggac
     3541 agtcagtggg tggtccgaca gttcggctac gagacgggcg gcaccccacc tcgtgcggtc
     3601 ggcgtgagcc cggctcaacg ccacttgatg ccgcacccga tcgacggccg ctggtcgggt
     3661 tcgaccggcc gcccggcgag cacggcgtcg accgccgccc ggacgtcagc ggccgtcacc
     3721 ggtaggtctt tccgtgggcg ggagtcgtcg agctggccgc ggtagacgag tcgacgctcg
     3781 ccgtcgaaga cgaacgtgtc gggcgtgcac gccgcagaga aggcgcgggc gacgtcctgg
     3841 gtctcgtcgt agaggtacgg aaacgtccag ccgtggcggc gggcctcggc gaccatctga
     3901 tcgggtccgt cctgcgggta ggtgacgacg tcattgctgg agatgccgac catcgcgacg
     3961 ccccgatcgg tgaggtcccg accgagcgcg gcgagcccgg cggcgacgtg ctggacgtac
     4021 gggcagtggt tacagatgaa ggtgacgaca agcgcgggac ctgtgagctc gtcgaggcta
     4081 accgtggcgc cggtggcggg ctcagggagc gcgaaccgcg gcgcaggggt gccgagggcg
     4141 agcatagtgg actcggtggc catgctcgcc agagtaggtc cactggcctg gcagatgaac
     4201 ggccagccgt ggattcgacc acggcctcga cgtaaggggc gctcacatga ccgggcacat
     4261 tccgctgaat ccacagcagc tcgacgctga gaaggtggtt ctgttgcaga cccgcaaacg
     4321 cgacggcagc tgggtggaca ccccggtcaa cattgcggta cggggcgatc gtgcgtactt
     4381 ccgcaccccc gggaaggcgt cgaagaacaa gcggctgcgc aacttccccg aggtgcagat
     4441 ccggccctgc acatggtcag ggaagccaac cggcggcact actatgaccg cgcacgcccg
     4501 gctgctgtcc ggcgaggagt ccgaggcggc tgggcggctg atcgaccgga agtacccggt
     4561 actacagcgc ctactggtgc gactgacgca caagatcatg cgcacgccga cgctgcatta
     4621 cgagctcgcc ccgtagcgcg aattacccgt tcggtatgga cttgacacga atcaagaaca
     4681 acctcaggtc agatcctcaa ttgcaggaag tcagacgggc ggcagtgttg ctgccgaagt
     4741 caagtccgtg attccggcga gtactcgtta tgaatccctc gtcgaaaatt gtcgaattcg
     4801 ggccacgacg ccgccggatc gctcacgtcg ccagtaggta gccggatgcc acgctgcgcc
     4861 ggtccaaccg gcgaaggccg agttcgtcgg cgagctccgc cgcacgcacg tactcgtctc
     4921 ggtgcagatg ccggttgatc tccgggtacg gatcccgtac atcggtacgc ccgaccaggc
     4981 cggccgggaa gtactgggcc atcaggtcga cgtaggtgtc gggtccgagc atgccggcga
     5041 tccaacgcag aatctctgcg gtttcgtcga acatcccggg catgaccagg tggcgcacca
     5101 acaaaccgcg gaccgccagg ccgtcgtggt caaggacgag cttcccgacc tgtcggccca
     5161 tctctgggat ggttgcgcgc atcacgtccg gataatccgg catgcgcagg aaccggcggg
     5221 cccgttcgcg gctccacacc ttggcgtcgg gcatgtagat gtcgacgatc ccgtccatca
     5281 gcacaaggct gtcgaggctg tcgtacgcac tggtgttgta cacgatcggc agccgcaggc
     5341 cagcgtcgat cgcgtgcggc agcgcctcga ggatctgtgg gaccacatgt tcgggcgtca
     5401 cgaagttgat gttgtggcaa cccttgtctt gaagctccaa catcatctgc gctagccgtt
     5461 gcggggtaac ctgctccccg cacagctccc aggagatgtc gtaattctgg cagaacacgc
     5521 agcgcaggtt gcagccggag aagaagatcg tgcccgagcc gttcctccca cgcaggcagt
     5581 tctcctcacc gaaatgcgcg aagtgcgacg caacaacagc cgtacgcccg atccgacaca
     5641 ggcctttgac gtcactcagc cggtcaaccc ggcacagtcg cgggcataca cgacaccggt
     5701 cgggaccgag cagaacccga gcctgcgcag cccgccgaga gatctcgtcg acgccgaggc
     5761 cgacatacgc ggggcacggt cggacgctgt ccatggatca ctctcaggct cggatcctac
     5821 gcggccatgg agcggtttgg cgacacgctg cggccgtgcg tgcggcgctg tgaatgaaca
     5881 cccttgcggc cgatgaccag tcggcgtaaa cgtcacctca gcggtgttgg tcagctgtgc
     5941 tgtggtggcg gtcgagatcc agaagtgccg cgaggaggtg c
//
LOCUS       DASRJA010000106        25603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1882316,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000106 DASRJA010000000
VERSION     DASRJA010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 25603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25603
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1882316"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..422
                     /locus_tag="VFC01_04840"
     CDS             114..422
                     /locus_tag="VFC01_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79006.1"
                     /translation="MGELQSIGYTGQPVQQAFRRVDPPVAVLVDLAALFPREPHRAGG
                     YNPAGLQMHSVVEGRLTCWGICEQGYWWGLVTYDIVYGARRKSVTHWIPAWMLKRKAD
                     "
     gene            complement(403..726)
                     /locus_tag="VFC01_04845"
     CDS             complement(403..726)
                     /locus_tag="VFC01_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HZN79007.1"
                     /translation="MSSSKQQIIAALDAAEASYRALAALPLEALTRPEKTELLKRLGE
                     IDRKMVALDRRLIGQLITAGDPAMFGGTSWADVLSRRLRISPGEAQRRIAEARSAKSL
                     SRPSA"
     gene            complement(766..2295)
                     /locus_tag="VFC01_04850"
     CDS             complement(766..2295)
                     /locus_tag="VFC01_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZN79008.1"
                     /translation="MRSYDYVVIGAGSAGCAVAARLAAASHSVLLIEAGGSDRRLPVR
                     APLAYGAQMGGKTDWAFESEPEPGCNGRRIPQPRGKVLGGTSAMNAMVWVRGTRDDYD
                     GWQVPGWGWDDVEPVFHRIESGPMRVTRVAEPDEVSRRFVAAARAAGVCANDNISGPE
                     LDGAAISPVTVWKGQRWSTARAYLDHARRLENFAVVSSALVRRIVIRDGAAVGVEYER
                     RGRSFTATARREIVLSAGAYGTPQLLQLSGVGPAEHLRSVGIDPIVGSPRVGHNLTDH
                     PATFMNWGLAPGFIGLADARNPKWLLRWLIRRSGKLTSNLMEAVAQIRSTPDLAAPDF
                     QLIHAPIYARIDEMSYSRPALSILQSYWTPESRGSVMVRSSDARDAPAIQLNTLTSAG
                     DVAAFVRAIRRTREIVSTDPLASAVAVEVHPGPNVNTDEEIAAWVRATVATTGHPACT
                     AAMGTEPDSVLDPQLRVRGVRGLRVADASALPRIPRANTNAPAIMVGERCADFMLSAS
                     G"
     gene            complement(2292..3071)
                     /locus_tag="VFC01_04855"
     CDS             complement(2292..3071)
                     /locus_tag="VFC01_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HZN79009.1"
                     /translation="MAHDPVGALRRERSELLRFCADLSLADWQTPSAADGWRVQDVVA
                     HMGSAYRALFGPAALELMRSNDIERTNDALVDRRRDWPPSRVLTEYERWSQVMLRMMG
                     PVSRTPLARAPMRLAELGTFRASQLLCAIVFDTHTHLRHDIAPALGRPVPDTDGDRMA
                     VVLEWMMAVLSNQLRAARHEWLDRPVSITLSGPGGGCWVVRPDGGIDLGSPAVSAAQI
                     DGVALEFPEWGTQRVSWRDRDVHIGGDADYGARFLDAVNVV"
     gene            complement(3103..3588)
                     /locus_tag="VFC01_04860"
     CDS             complement(3103..3588)
                     /locus_tag="VFC01_04860"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZN79010.1"
                     /translation="MTKAFEKLVSLLDYPMFVVTTRTADESAGCLVGFASQVSIHPRR
                     FLVGLSKRNHTYRVAKGASHLAVHLIERRHSELARLFGGETGDETDKFGRCAWHSGPH
                     DLPILDDAAGWFAGVVLNCYDVGDHVGFLLEPIDGTAPDTLQQLVTFSDVRDLDPGHE
                     A"
     gene            3612..4067
                     /locus_tag="VFC01_04865"
     CDS             3612..4067
                     /locus_tag="VFC01_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZN79011.1"
                     /translation="MARKYATADAVSLPDLLEFIRPRHRMVLTTFRSDGSLQSSPVSG
                     GVDDAGRIVIASYPQRAKSANVRRTPRASVMVLSDEFNDAYVQIDGDAEVIELPDAVE
                     PLVDYYRSIAGEHPDWAEYRRAMVDQGKCLIRVTPRRWGPVATGGFPPQ"
     gene            complement(4070..4459)
                     /locus_tag="VFC01_04870"
     CDS             complement(4070..4459)
                     /locus_tag="VFC01_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HZN79012.1"
                     /translation="MSRHIEARFEIASWDETPFEDGDEATKLTEALVAKRYEGDIKGT
                     STTKWLMAYAPDKTALFVGVEHITGTIGGKDGGIVLIHDGEYRDGVAAAELRVASGTD
                     GLATVAGTGKFRADPAGSMTLDLDGAW"
     gene            4682..5908
                     /locus_tag="VFC01_04875"
     CDS             4682..5908
                     /locus_tag="VFC01_04875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN79013.1"
                     /translation="MRIALLSYRSKTHCGGQGVYVRHLSRGLVELGHDVEVFSGQPYP
                     EVLDPRVRLTKVPSLDLYREPDPFRIPRPSEIKTRIDLLELLTTWTAGFPEPKTFSLR
                     AARLLAGRRDDFDVVHDNQCLGTGLLTIAELGFPVVATVHHPITRDRVLDVAAARWWR
                     KPLVRRWYGFAEMQQTVARQIPELVTVSSTSAADIAEDFAVASDQLHVVPLGVDTALF
                     KPAQRRVRNRIIAIASADVPLKGVSHLLHAVARLRVERDLELQLVAKLEPNGPTEKLI
                     AELGISDIVHSSSGLSDSELADLLASAEIACIPSLYEGFSLPAVEAMASGTPIVASRA
                     GALPEVVGADGECARLVRPADVDELTRVLGELLDSPTELRRLGAAGRRRALEVFSWES
                     VAAQTVSVYEKAMAAC"
     gene            5902..6633
                     /locus_tag="VFC01_04880"
     CDS             5902..6633
                     /locus_tag="VFC01_04880"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN79014.1"
                     /translation="MLTVDYDRLGVGAGTKVIDVGCGAGRHSFEAYRRGADVIAFDQN
                     AADLNDVDEILQAMKDQGEVPESARAEAVKGDALDLPYDNSTFDCVIASEILEHVPED
                     DRAIAELVRVLKPGGSLAITVPRWLPEKVCWILSDEYHANEGGHIRIYRADALRDKVL
                     AHGLHLTHTHHAHALHSPFWWLKCAVGTWNNDHPAVAAYHRMLVWDMVGQPWLTRTAE
                     SLLNPLIGKSVALYFRKPVVSVAGS"
     gene            6620..7690
                     /locus_tag="VFC01_04885"
     CDS             6620..7690
                     /locus_tag="VFC01_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HZN79015.1"
                     /translation="MLAPDVPGVPGALTPAQCRQTAKSIAATQESSGAIPWFDAGHTD
                     PWDHVENAMALTVAGLLEPARAAFDWCRRAQRPDGSWPIQLRNGVIEDANSDSNFCAY
                     IATGVWHHVLITRNRRFAETMWPVVTKAIDFVVGLQASGGEISWARSPSGPEPDALLT
                     GCASIYHSIRCALALADYFDDPQPEWEVAVGQLGHAIAHHPDAFVTKDRWSMEWYYPV
                     LGGALRGAAARSRIDERWDDFVVPGLGIRCVDDRPWVTGAETCELVMALDAIGDSARA
                     HEQFAAMHHLREEDGSYWTGLVYADGKRWPVERTTWTGAAMILAADALSRTTPANGIF
                     RGADLPRGLEGEYDCACATSDR"
     gene            complement(7687..8553)
                     /locus_tag="VFC01_04890"
     CDS             complement(7687..8553)
                     /locus_tag="VFC01_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZN79016.1"
                     /translation="MAGLMPRALADPEADVVAPPDPPPAAPIEVFALPLPLAIPDGTP
                     VGQDPTPFTGIAPFDPPVINPSDTSMVGVAMPIIVDFGTSVADRAAAEQAIHVSSDPP
                     VPGKFYWMNDSQVRWRPFDFWPAHAVVNVDAAGTKSSFVTGDALVATADDATHQLTIT
                     RNGDVEQVFPMSMGKPGHDTPNGTYYVQGKMRDVVMDSATYGVPNTSPDGYRLHVQLA
                     VQFDNTGNFVHSAPWSAGDQGLRNVSHGCINISPSNAEWFYNNFGVGDPIVVTNSVGL
                     YAQNDGAQDWQI"
     gene            8730..10133
                     /locus_tag="VFC01_04895"
     CDS             8730..10133
                     /locus_tag="VFC01_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015385234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN79017.1"
                     /translation="MTGRTSDNLLIVHWHDTGRWLGAYGHPGVSSPCVDRLAAEGIRF
                     TRAHAASPGCSPARGAIFTGRYPHSNGLIGLAHHGFEYREGVRTLPHILSEAGWYSAL
                     FGMQHETSYPSRLGFDEFDVSNSCCEYVVDRAQEWLVESAPLEEGDPFLLTAGFFETH
                     RPWSRARYEPAPLDDVEVPAYLPDIAEVDADIADFYGAIAVADAAVGRLLDTLAETGF
                     DVNTWVVFFSDHGEPFPRAKSMLYEAGTGISLILRPPTRLGIAPGVYDELFSGVDLLP
                     TLLDLLGIEIPADIEGISHAGNLLRPATDAAPVRERVFTEKTYHDSFDPARAVRTKEY
                     SYIENYVPRHLVDLPLDVQDSPSGKVVAPFVAGPRPARELYDLREDPAELNNLLAGEV
                     SEPVEQIATELAILLHEWRERTGDVCPSEFAGPRILVEVSETYIEATMRTLGVRPTSQ
                     SPMGIHRGLRELTGTSG"
     gene            complement(10130..11452)
                     /locus_tag="VFC01_04900"
     CDS             complement(10130..11452)
                     /locus_tag="VFC01_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN79018.1"
                     /translation="MTTIEREAPTAVVEMPPGPPLPRAVQGVIAVANRRIALQMLRRR
                     YGSAFTIDLPIFGQMVVISDPSHIRQLFRTAPDIADTTDANLGRVMGPNSMFALMGER
                     HRAQRKLLTPSFNGRRLAAYEAIIEREAADEFATWPEDRTFAAMPSMMRITLNVILRA
                     VFGAEGHELERLREILPPGIRLGSMLALAPVPQWDWGRWSPWGRFYQYRREYDAIVDR
                     LIDKALADSALGERDDVLALMLQSRYEDGSPMSRAEIADELITMLAAGHETTATTLAW
                     AVERLQRHPQLLDRLVADLDAGSEELLNATIFEILRTRPVIDTTFRQVKAPTLRIGPW
                     TVPQGQTIVASIGLLHADESVFPDPDRFDPDRFISRRPDPAEWIPYGGGVRRCIGAAF
                     ATMEIRVVLRTLWRNYTIHPSTARDEGRQSRGVAIAPSRGALVRVRRR"
     gene            complement(11510..12157)
                     /locus_tag="VFC01_04905"
     CDS             complement(11510..12157)
                     /locus_tag="VFC01_04905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN79019.1"
                     /translation="MSRTDELFALAEQVTGFMPADEGRTLFDTAVRYLGDGAGVEIGT
                     YCGKSTVLLGAAAQQSGGVIYTVDHHHGSEEHQAGWEYHDASMVDAVTGLFDTLPTLR
                     HTLDTAGLDDYVVAIVGKSPVVARGWRMPLRLLFIDGGHTEEAAQRDFDSWARWVDVG
                     GALIIHDVFPDPNDGGQAPFHIYQRALSTGDFREVLATGSMRVLERTSGTPGQQL"
     gene            12212..12781
                     /locus_tag="VFC01_04910"
     CDS             12212..12781
                     /locus_tag="VFC01_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN79020.1"
                     /translation="MSDPVLESTRRRLTAKQADTVDRLGRAALDLLSREGFAGLTVRR
                     VAAEAGVGAATAYTYFSSKEHLVAEVFWRRLAAAPPAAHDSGDAAARVVDVLREISLL
                     VADEPEFACAVTNALLGKDPDVEVLRLRIGRDIRDRLAAALGPDTDPDIIDSLDMLYS
                     GALVRAGMGYASYADIAQRLEKSARLMLR"
     gene            complement(12778..13377)
                     /locus_tag="VFC01_04915"
     CDS             complement(12778..13377)
                     /locus_tag="VFC01_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN79021.1"
                     /translation="MTTALDKAASVDDAFRDRLLDGLAASIGERGYRDTTVADIVRHA
                     RTSKRTFYEQFASKEECLIELLRRNNEDLIASIQAAVDPEADWQQQIRQAAGAYVDHI
                     GSRPAITLSWIREAPALGAVARPLHRLAMEALTDMLVDLTDSPGFRRASLAPISRPLA
                     LILLGGLRELTALFVEDDRDVQGIVEPATAAATAILGPR"
     gene            13484..14830
                     /locus_tag="VFC01_04920"
     CDS             13484..14830
                     /locus_tag="VFC01_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN79022.1"
                     /translation="MTETAVTAEATTPTTVGKMPPVVRLPKAVQMVLMSAFRRPFLQY
                     ALKRYGPVFAINVPFFGRSVVVSDPALARQVFLASTDDLINVQPNLSRIFGPGSVFAL
                     NGKEHRARRKLLAPPFHGQSIKNYEKVIEEETLRETAMWPEGVEFRTLEPMNRITLNV
                     ILRTVFGAEGAELDFLREIIPPWAKLGSRMATLPEPPFSTGRRSPWGRLEEFRRNFDR
                     TVFALIHKAHADPHLDERTDILALLLRSTYEDGTPMSRQDVSDELLTLLGAGHETTAS
                     TLGWAFERLRRHPDVLAKLVDENDDGGNEFRQASIQELQRNRTVIDFSGRHVQAPHFD
                     LGDWRIPHGYTVMIAIANVHANADVFPNPERFDPDRFVGTRTPTAWIPFGGGTRRCIG
                     AAFANVEMDVVLRTILRHFVIETDDAPEEKIHFRGIAFTPKDGGRIVVRRRESAAA"
     gene            complement(14827..15342)
                     /gene="msrA"
                     /locus_tag="VFC01_04925"
     CDS             complement(14827..15342)
                     /gene="msrA"
                     /locus_tag="VFC01_04925"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZN79023.1"
                     /translation="MTEYKKAILAGGCFWGMEELIRKQPGVVSTRVGYTGGENANATY
                     RNHPGHAEAIEITYDPSQTDFRNLLEFFFQIHDPTTRNRQGNDVGTSYRSAIFYADDE
                     QKRIALDTIADVDASGLWPGKVVTEVTPASDFWQAEPEHQDYLQRFPTGYTCHFPRPG
                     WKLPKRETQQA"
     gene            complement(15339..15809)
                     /gene="msrB"
                     /locus_tag="VFC01_04930"
     CDS             complement(15339..15809)
                     /gene="msrB"
                     /locus_tag="VFC01_04930"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZN79024.1"
                     /translation="MSHQYNKNPAAVGALSPEQYNVTQKNGTERPFTGEYWDNHDPGI
                     YVDVVSGEPLFASVDKFDSGTGWPSFTKPIDAENVVEKRDFSHLMIRTEVRSAHGDSH
                     LGHVFTDGPRAEGGLRYCINSASLRFIHLDDLEAEGYGDYKALFSKSASKEESA"
     gene            15870..16268
                     /locus_tag="VFC01_04935"
     CDS             15870..16268
                     /locus_tag="VFC01_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79025.1"
                     /translation="MRSVVLAVCVVLAAWSAPSASADPVAPPEATDAEGVAFTDNPAI
                     VDPHPMPVESWSRVGNDRAVAVHFTTGTPACNGVHATAHETADTVTIDLKGGSLPASA
                     GRMCIMLAVTGTLVVPLQSPLGDRQVLSAA"
     gene            complement(16255..17103)
                     /locus_tag="VFC01_04940"
     CDS             complement(16255..17103)
                     /locus_tag="VFC01_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN79026.1"
                     /translation="MKALLGLAFALAVFAGCAPNPHEPDFAGLVYIGNGRQLFLNCQG
                     TGSPTAFIIPGKGSYAAEWNVVVPVLAKTTRVCAYDRPNTRPDGPERSTPVPQPHTVQ
                     QDVNDIVKLIVAADLSTPMVVVSHSYGALIADLLSRTHPEFVSGLVFVDPTSEYLPRL
                     GRPDQDTEFDREARAPAPNAHGEGFLAADAFARLKAAPAPPLPRVPAVVLSSDKFPPP
                     TDLTPQSYTKFQVHRANSVLAETLWADNLIVAGSGHDVMLYQPQVVADKIVAIVEKVR
                     LGPMPR"
     gene            17164..17670
                     /locus_tag="VFC01_04945"
     CDS             17164..17670
                     /locus_tag="VFC01_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN79027.1"
                     /translation="MTSKTFTRDELAAAFETFEATVDRAAQSRDWDPWVEQYTPDVTY
                     VEHAAGTMHGRDEVRAWIWKTMETFPGSYMTSFPSLWSVIDEPTGRIICELDNPMRDP
                     GDGTIISASNISILTYAGDGHWCRQEDIYNPLRFVSATRKWCRKAQELGTLPDEAAAW
                     LRQFGDLR"
     gene            17667..18677
                     /locus_tag="VFC01_04950"
     CDS             17667..18677
                     /locus_tag="VFC01_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN79028.1"
                     /translation="MSAKLVIGANGFLGSHVTRQLIADGHEVRAMVRPNAMTVGIDDL
                     PVTRFHGDIWDNDTLREAMTGVDDVYYCVVDARGWLRDPAPLFRTNVDGTRNVLEIAK
                     DAGLHRFIYTSSYVTVGRRRGHIASEDDTIVDRGLTPYVRSRAQAENIVLKYATQHGL
                     PAVAMCVSTTYGSGDWGRTPHGAIIAGAAFGKLPFVMSGIELEAVGVDDAARAMILAA
                     ERGRQGERYLISDKMISNADVVRIAAEAAGVPAPTKSIPLPVSYALATAGSFKAKLRG
                     TDERLSLGSLRLMRAEAPVDHGKAVRELGWEPRPVEESIREAARFWVGLRDAKRKSKT
                     AN"
     gene            18732..19040
                     /locus_tag="VFC01_04955"
     CDS             18732..19040
                     /locus_tag="VFC01_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79029.1"
                     /translation="MGEDQAPTDAPGTEPGGNPSGAKIGEALASELSRVVAAYTQQIQ
                     EALQTCLSQLTEQWQSAFAVYAAEYTKQWQEAMRAVAVQQTHGAPASESFEEKQGEAE
                     "
     gene            19112..19915
                     /locus_tag="VFC01_04960"
     CDS             19112..19915
                     /locus_tag="VFC01_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79030.1"
                     /translation="MKLDARSSGTPTVSADPIEVELPDITVTVPEITVELPNPTLNVT
                     FGVGDSGVVEYLEGVRAFLQSLDPLDPSDVALGAAALLAIGPDLIRAVALPKLELEAE
                     LEFGELKISADEALAIDTGRIRLHGVPATDDVLPVKIAADLDETGVTALLDGMRATLT
                     GCLVLNGGDPPPAPPAPPATVQGVDVKVDANKKLLVLIIHGQGFGPVQGTVVLSQVGL
                     SLTPVQFVSWTDQEIEVIFQPIPPPGAYAVTVTTAQGASTQPLPVTVSN"
     gene            complement(19912..20853)
                     /locus_tag="VFC01_04965"
     CDS             complement(19912..20853)
                     /locus_tag="VFC01_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN79031.1"
                     /translation="MRIDVHAHYWTDAYLDMLVDLGRSDTATQRGIGAGGGAELDARL
                     RLMDRAGVDIQVLSAAPQLPYGDDVARTAAAARYVNDEYAALVSSHPDRFRAFAATPM
                     PHIDASITEMSRAIDELGMSGLTMNTSILDRAITDPEFEPVFAELDRRGAILYLHPVG
                     NGACSPLVSEHHITWTVGAPFEDTIAAMQLITSGHLQRFPNVRIICSHLGGALPMIPL
                     RADDHVAWEAPDTPEAPSHAVHRLWYDTVSHCHAPALRCAIDSFGADRILLGTDFPYE
                     DGDTFVRAVEYIIDATDPAEAHAILDANAMALFRISG"
     gene            20987..21760
                     /locus_tag="VFC01_04970"
     CDS             20987..21760
                     /locus_tag="VFC01_04970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN79032.1"
                     /translation="MLATFYAKAVDADLPQPILGDRFAKDIVDRVDYDWKKTSITATN
                     SPAVTTRSAHFDNWARQFLAVHPHAVVLHLGCGLDARFFRLAPGPGVDWYDIDYPDVA
                     HLRQQLYPTRDHYRVIAASVTDPSWFAEIPRDRPTLMVGEGLTMYLTQEDGIALLRRV
                     VEHAPSGELQFDAFNRLGIKAQWTNTVVRRSGATLHWGIDGPDDIVTAVPGVRLLSWV
                     SAIDGDAFARVPWYYRWTLKAMRIAPVLRYMAQYHRYAF"
     gene            complement(21748..22215)
                     /locus_tag="VFC01_04975"
     CDS             complement(21748..22215)
                     /locus_tag="VFC01_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HZN79033.1"
                     /translation="MCWQCDNPNGTTEEYLDELRALIRIHGWAVQGVEDDRLPYAYTI
                     GLHDRGLPELLVTGLSQERAARVLNDVAVAAVGGRVFQPGAHIAVGDGPLLEIVEVDH
                     PDAHMNFAVALGGPEIRALQLVWADDRGRWPWAAGWGHGRRRQPVLGVRAQKA"
     gene            complement(22237..22701)
                     /locus_tag="VFC01_04980"
     CDS             complement(22237..22701)
                     /locus_tag="VFC01_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HZN79034.1"
                     /translation="MRSPEATHDDYCDLLQRKIRKKGWTVQYVESDRRPYTYTIGLHD
                     WDVPELLMTGVSPQRALRLLDIVARRLVGGDVFAPGQQTSLPDGPLIEIVEVDHPDAH
                     MGWAVAVGGPEIRALQLVWADGRGRWPWSAEFADGRAMQPVLGCRTPGASWP"
     gene            22820..23059
                     /locus_tag="VFC01_04985"
     CDS             22820..23059
                     /locus_tag="VFC01_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN79035.1"
                     /translation="MLVDHPMTLTIPVSDLERARAWYRDKLGFNPVHEVPGEALAYRT
                     GSGQSLLLFSSDEAGAVEHQLAAWRVDDVEAEVTS"
     gene            complement(23056..23529)
                     /locus_tag="VFC01_04990"
     CDS             complement(23056..23529)
                     /locus_tag="VFC01_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN79036.1"
                     /translation="MTEQEPASAQADVTIDADPQAVYALITDLPTLAACAEEAEAMKW
                     RKGDAVRPGAVFKGYNRNGSRRWTTVCTVTDAQPGRAFAFDVKTAAVVPVARWRYDIE
                     PVEGGCKVTERTWDRRPGWIRKPAQFTTGVKDRTAANAEHIKLTLQRLKGKAEAR"
     gene            23589..23963
                     /locus_tag="VFC01_04995"
     CDS             23589..23963
                     /locus_tag="VFC01_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HZN79037.1"
                     /translation="MSDRPVLEPTASHPITVEPTGKHVTVRINGELVADTDNALTLQE
                     STYPAVQYVPLGDVAAERLRPSDTETYCPYKGDASYYHVVTEAGGTVDDAIWTYEKPY
                     PAVGQIAGHVAFYPDKAEISVA"
     gene            23973..24884
                     /locus_tag="VFC01_05000"
     CDS             23973..24884
                     /locus_tag="VFC01_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HZN79038.1"
                     /translation="MHRSVSVIFDGPASPGLTLSPLRRGRGDPCYHDAQDGAIWRTSL
                     MRSGPVTARITKSAPDTVDCEAWGSGATEFTETLPALLGADDDASGFAPDEPTIAAAH
                     QRMPHLRLGRTDRVIESLIPAILEQRVYGMDARRAWRALVTKFGSSAPGPAPEHMRVP
                     PAAEVWRRIPSWEFHRANVDPGRARTIVGCAQRADSLERLATRPPEQARAAMMSLPGV
                     GEWTAAETAQRAFGDADALSVGDYHLASMVGWRLLGAPIDDPAMVELLEPLRPHRHRA
                     VRLLEVTGPAHAPRRAARQAIPNLRAL"
     gene            24924..25481
                     /locus_tag="VFC01_05005"
     CDS             24924..25481
                     /locus_tag="VFC01_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HZN79039.1"
                     /translation="MTEFTIPGISEKDANEVADILQKQLSTYNDLHLTLKHVHWNVVG
                     PNFIGVHEMIDPQVETVRAYADVSAERIATLGKSPQGTPGAIIKDRTWDDYSVGRDTV
                     QAHLAALDLVYTGVIEDTRKAIHRIGELDSVTENILEEHAAELEKFQWFVRAHLENAG
                     GQLASAGAKTERAAASKAKKKAPVK"
     gene            complement(25474..>25603)
                     /locus_tag="VFC01_05010"
     CDS             complement(25474..>25603)
                     /locus_tag="VFC01_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HZN79040.1"
                     /translation="FWLAPLLGAAIAGIAYPYLFGRNEELADRPVRDDALEEPATT"
BASE COUNT         4361 a         8415 c         8419 g         4408 t
ORIGIN      
        1 cggccagcgc cgcgctgatc agatcgcctc ggtgaggaca ctgtccacga ggcaaacgcc
       61 ctgaccagca ctgatgagta cacgtgagac ccaagcgaac gtatgatcga acagtgggtg
      121 agctccagtc gatcggctac accgggcagc ccgtacaaca ggcgttccgg cgggtggacc
      181 cgccggtcgc ggtgctggtc gacctggccg cgctgtttcc ccgcgagccg catcgcgccg
      241 gcggctacaa ccccgctggt ctgcagatgc actcggtcgt cgaagggcgg ctcacctgct
      301 ggggcatctg cgagcagggc tactggtggg ggctggtcac ctacgacatc gtctacgggg
      361 cacggcgaaa atccgtcacc cactggatcc cggcatggat gctcaagcgg aaggccgact
      421 gagtgattta gccgaccgtg cctcggcgat ccgccgctgt gcctcgccgg gtgatattcg
      481 cagccgtctg gacagcacgt ctgcccacga agtcccgccg aacatcgctg gatcgcccgc
      541 agtaatcagc tgcccgatca gccgccggtc gagagccacc atcttcctgt cgatctctcc
      601 cagccgcttg aggagttctg tcttctcagg gcgcgtcagc gcttccagcg gaagcgcggc
      661 aagtgctcgg tagcttgctt cggcggcatc gagcgccgcg atgatctgtt gtttactcga
      721 actcatgttc gaatgatatc gccccgaacc gacacgtgac gaaatttatc cacttgccga
      781 gagcatgaag tctgcgcagc gttcgccaac catgatcgcc ggcgcgttgg tattggcccg
      841 agggatgcgc ggtagcgccg aggcgtcggc cacccgcaga ccgcggacgc cgcgcaccct
      901 cagttgcgga tccagcacgc tgtccggttc agtgcccatc gccgccgtac acgccggatg
      961 cccggtcgtc gccaccgtcg cccggaccca cgccgcaatc tcctcgtcgg tgttcacgtt
     1021 cgggccgggg tggacctcca ccgccaccgc tgacgccagc gggtccgtcg acacgatctc
     1081 gcgcgtgcgc cggattgccc ggacgaacgc ggctacgtcg ccggcagaag tcagcgtgtt
     1141 cagctggatc gccggggcat cccgggcatc ggacgaccgg accattacgc tgccgcggct
     1201 ttccggcgtc caataggact gcaggatgga cagtgccggc cgcgaataac tcatctcgtc
     1261 gatgcgcgca tagatcggcg cgtggatcag ctggaagtcc ggcgcggcca aatcaggagt
     1321 cgaacgaatc tgcgcgacgg cttccatcag gttgctcgtc agcttgcctg accggcgaat
     1381 cagccagcgc agcaaccact tcggattgcg cgcgtcggcc agcccgatga aacccggcgc
     1441 gaggccccag ttcatgaacg tggccggatg gtcggtgagg ttgtgcccca ccctcgggct
     1501 gcccacaatg ggatcgattc cgaccgagcg gagatgctcg gcgggcccga caccggacaa
     1561 ctgcaacagt tgcggcgtgc cgtatgcgcc tgcgctgagc acgatctcac gtcgcgctgt
     1621 cgcggtaaac gaccgcccgc ggcgctcgta ctccacgccg accgccgcgc cgtcgcggat
     1681 cacgatccgg cgcaccagcg cactgctgac cactgcaaaa ttctcgagac gccgcgcgtg
     1741 gtccaggtag gcgcgtgcgg tgctccaccg ctgtcccttc cacaccgtga cgggtgagat
     1801 cgcggcgccg tcgagctcgg gtccgctgat gttgtcgttg gcgcatactc cagccgcccg
     1861 ggcagcggcg acgaaccggc gcgaaacctc gtcgggctca gccactctcg tcacccgcat
     1921 cggtcccgat tcaatcctgt ggaacaccgg ttcgacgtcg tcccagcccc atccaggcac
     1981 ctgccagccg tcgtagtcgt cgcgggtgcc gcgcacccag accattgcgt tcatcgcgct
     2041 ggtgccgccg agcaccttgc cgcgtggctg cgggatgcgc ctgccgttac agcccggctc
     2101 cggctcggat tcgaatgccc agtccgtctt gccgcccatc tgcgccccgt aggccagcgg
     2161 cgcgcgcacc ggcagtcgcc ggtccgaccc gcctgcctcg atcagcagca ccgagtgcga
     2221 cgccgcggcc aaccgcgcgg ccacggcgca gcccgcggag ccagcaccga tcaccacgta
     2281 gtcgtagctc ctcacaccac gttcaccgca tcgagaaacc gggcaccgta atcggcgtcg
     2341 ccgccgatgt gcacgtcgcg gtcgcgccag ctcacccgtt gcgtgcccca ctccgggaac
     2401 tccagtgcga caccgtctat ctgcgcggcg ctcaccgcag gtgaccccaa atcaataccg
     2461 ccgtcgggtc gcaccaccca gcagccgccg cccggccctg acaatgtgat cgacaccggc
     2521 cggtccagcc actcgtgccg ggccgcgcgc aactggttgc ttagcaccgc catcatccac
     2581 tcgaggacca cggccattcg gtcgccgtcc gtgtcgggga cgggacggcc tagcgccggc
     2641 gcgatgtcgt gccgcagatg agtgtgcgtg tcgaacacga tcgcgcacag cagctggctc
     2701 gccctgaacg tgcccaactc ggccagccgc atcggagcgc gagcaagcgg agtgcgcgac
     2761 accggaccca tcatgcgcag catcacctga ctccaccgct cgtactcggt caggacacgc
     2821 gacggcggcc aatcgcggcg gcgatcgacg agcgcgtcgt tggtgcgttc gatgtcgttg
     2881 ctgcgcatca gttccagtgc ggccggcccg aacagtgcgc ggtacgcgga acccatgtgg
     2941 gctacgacgt cctgcacccg ccagccgtcg gccgcgctcg gcgtctgcca gtcggcgagg
     3001 gagaggtcgg cacagaacct cagtaactcc gagcgctcgc gccggagtgc gccgaccggg
     3061 tcgtgggcca ctcgaatacc ttacagtaag ccgcccaatt agctaggcct cgtggcctgg
     3121 atcgaggtcg cggacatcgg agaacgtgac gagctgctgt aacgtgtccg gcgcggtccc
     3181 atcgatgggc tcgagcagaa atccgacgtg atcgccgacg tcgtagcaat tcagcaccac
     3241 gccggcgaac caccctgccg cgtcgtcgag aatcggcaag tcatgcggac cggaatgcca
     3301 ggcacagcgg ccgaatttgt cggtctcatc accggtttcg ccgccgaaca ggcgggccag
     3361 ttcgctgtgc cgccgctcga tcaagtgcac ggcaagatgg ctggcgccct tcgctacccg
     3421 gtaggtgtgg ttgcgtttcg acagtccgac caagaaccgc cgcgggtgga tgctcacttg
     3481 actcgcgaaa ccgacgaggc agcctgccga ctcatcggca gtgcgggtgg tgaccacgaa
     3541 catcggatag tcgagcaacg aaaccaattt ctcgaacgct ttagtcatcg ctccgtcgct
     3601 agggttgacg gatggccaga aagtatgcga ccgccgatgc ggtcagcttg cccgacctcc
     3661 tcgagttcat ccggccgcga catcgcatgg tgctcaccac atttcgatcg gacgggtcgc
     3721 tgcagagctc tccggtgtcg ggcggtgtcg acgatgccgg tcgcattgtg atcgccagtt
     3781 acccgcagcg cgccaagtcg gccaacgtcc gtcgcacgcc gcgcgccagc gtgatggtgc
     3841 tgtccgacga attcaatgat gcctacgtgc agatcgacgg cgacgccgag gtgatcgaac
     3901 tgcccgatgc cgtggagccg ttggtcgact attaccgctc catcgccggc gaacaccccg
     3961 actgggcgga gtaccggcgg gcgatggtcg accagggcaa gtgcctgatc cgcgtcactc
     4021 cgcgccgatg gggtccggtg gccaccggcg gattccctcc gcaatagcgc taccaggcgc
     4081 cgtcgaggtc gagcgtcatc gagcctgcgg gatcggcgcg gaactttccg gtccccgcca
     4141 ccgtcgccaa cccatccgtg cccgacgcga ctcggagctc ggccgccgcg acgccgtcgc
     4201 ggtactcgcc gtcgtgaatc agcacgattc ccccgtcctt accgccgatc gtgccggtga
     4261 tgtgctcgac gccgacgaac agcgcggtct tgtccggcgc gtaggccatc agccatttgg
     4321 tcgtagaggt gcccttgatg tcgccctcgt agcgcttggc caccagtgct tcggtcagct
     4381 tcgtcgcttc atcgccatcc tcgaatggtg tctcgtccca gctggcgatc tcgaagcgcg
     4441 cctcgatgtg tctgctcatg acagccggat accccggacg agaggtcggg aatcgacggg
     4501 ccgatcagga cgctgtgcgc cggctgtggt agtcagagca aggtggccgc gttgcgtccg
     4561 ctagcacgtc aaactatagt ctggacacat gtccagaggg ttcggagttt ttgcgtgacg
     4621 ccatttgtcc agctcaccgg ccctcgaact tccgactgct gcgcgtcttg gtgagttgac
     4681 cgtgcgcatc gccttgttgt cctaccggag caaaacccat tgcggcggcc agggcgtcta
     4741 cgttcgccac ctgtcccgcg ggcttgtgga actcggccac gacgtcgagg tgttctccgg
     4801 gcagccctac cccgaagtgc tcgatccgcg ggtgcggctg accaaggtgc cgagtctcga
     4861 tctgtaccgc gagccggacc cgttccggat tccgcgcccc agtgagatca agacgcgcat
     4921 cgatctgctc gagctgctga cgacgtggac ggctggcttt cccgagccga agacatttag
     4981 cctgcgcgct gcccggctcc ttgccggccg gcgcgatgat ttcgacgtcg tgcacgacaa
     5041 ccagtgcctc ggcaccggtc tgctgacgat cgccgagctt ggtttcccgg tggtggcgac
     5101 cgtgcaccat ccgatcacgc gggaccgagt tctcgacgtg gctgccgccc gctggtggcg
     5161 aaagccgttg gttcggcgct ggtacgggtt cgcggagatg cagcagacgg tcgcccgcca
     5221 gatcccagag ctggtgacgg tctcgtcgac gtcggctgcc gatatcgcag aagacttcgc
     5281 ggtcgcgtcc gaccagctgc acgtggtccc gctcggcgtg gacaccgcgc tgttcaagcc
     5341 cgcgcagcgg cgggtgcgca accggatcat cgcgatcgcc agcgcggacg tgccgttgaa
     5401 gggggtcagc catctgttgc atgcagtcgc ccggttgcgt gtcgaacgcg atctcgaact
     5461 gcagttggtc gccaagctcg aacccaacgg gccgaccgag aagctgatcg ccgaattggg
     5521 catctccgac attgtgcaca gctccagcgg gctctccgac tcggagctgg ccgatctgct
     5581 cgcatcggct gaaatagcgt gcatcccttc gctatacgag ggtttctcgt tgcctgcggt
     5641 cgaagccatg gccagcggca caccgattgt cgccagccgc gccggtgcgc tgccggaggt
     5701 ggtcggcgcc gacggcgaat gcgcacggct ggtacggccg gccgacgtcg acgagctgac
     5761 gcgcgtgctg ggcgagctgc tggactcccc gacagagctg cgccgcctcg gtgcggcggg
     5821 acgtcgtcgc gcgctcgagg tgttcagttg ggaatccgtt gcagcgcaaa ccgtctcggt
     5881 ctatgagaag gccatggcgg catgctgact gtcgactacg accggctggg cgtcggcgcc
     5941 ggcaccaagg tgatcgatgt cggttgcggc gcgggccggc attcgttcga agcgtaccgc
     6001 cgcggtgccg acgtcattgc gttcgaccag aacgcggccg acctcaacga cgtcgacgag
     6061 attctgcaag caatgaaaga ccagggcgag gtgcccgagt cggcgcgggc cgaggcggtc
     6121 aagggtgacg cgctcgactt gccgtacgac aacagtactt tcgactgtgt gatcgcgtcg
     6181 gagatcctcg agcacgtacc cgaggacgat cgtgctatcg cagaacttgt ccgggtgctc
     6241 aaacccggtg gctccctggc gatcacggta ccccggtggc tgcctgagaa ggtgtgctgg
     6301 attctgtccg atgagtacca cgccaacgag ggcggtcaca tccgcatcta ccgggctgac
     6361 gcgctgcgcg acaaggtgct tgcccacggc ctgcacctga cccacaccca ccatgcgcac
     6421 gccctgcatt caccgttctg gtggctgaaa tgcgctgtgg gaacgtggaa taacgaccac
     6481 cccgcggttg ctgcctacca caggatgctg gtgtgggaca tggtcggcca accgtggctc
     6541 acccgcaccg cagagtcgct gctgaacccg ctgatcggca agagcgtggc gctgtacttc
     6601 aggaagccgg tggtgtctgt tgctggctcc tgacgttccc ggcgtgcccg gcgccctgac
     6661 gccggcgcag tgccgccaga ccgcgaagtc gattgccgca acgcaggaat cctctggtgc
     6721 catcccgtgg ttcgacgccg gccacaccga tccgtgggat cacgtggaaa acgcgatggc
     6781 gctcaccgtc gccgggctgc tggagccggc ccgggcggcg ttcgactggt gccgacgtgc
     6841 gcagcggccg gacggatcct ggcccatcca gttgcgcaac ggcgtcatcg aagacgccaa
     6901 cagcgacagc aacttttgcg cctacatcgc caccggcgtc tggcatcacg tgctgatcac
     6961 ccgcaaccgg cggttcgccg agacgatgtg gcccgtagtc acgaaggcca ttgacttcgt
     7021 ggtcggcctg caggcgagtg gcggtgaaat ctcctgggcc agaagcccat cgggaccaga
     7081 acctgatgcc ttgctaaccg gatgcgccag catctaccac agcatccggt gcgcactggc
     7141 gctggcagac tacttcgacg atccccagcc cgaatgggag gtcgccgtcg gccagctggg
     7201 tcacgcgatc gcgcatcacc ccgacgcatt tgtcaccaag gatcgctggt cgatggagtg
     7261 gtactacccg gtgctcggtg gcgcgctgcg gggcgcggcg gcccggtcac gcatcgacga
     7321 gaggtgggat gacttcgtcg tgcccgggct cggcatccgc tgcgtcgacg accggccatg
     7381 ggtcaccgga gcggaaacct gtgagctggt aatggcattg gacgcgatcg gagactcggc
     7441 gcgtgcccac gagcaattcg cggccatgca tcatctgcgc gaggaggatg gctcctactg
     7501 gaccggactg gtgtacgccg acggcaagcg ctggccggtg gagcgcacca cgtggacagg
     7561 tgcggcgatg atcctcgccg ctgatgcgct atcgagaacc acgcctgcca acggcatctt
     7621 ccgcggcgcg gacctgcccc gcggcctgga gggcgaatac gactgcgcct gcgccaccag
     7681 cgaccgctag atctgccagt cctgcgcgcc gtcgttttgg gcgtacagac caacggagtt
     7741 ggtcaccacg atgggatcgc cgacaccgaa gttgttgtaa aaccattccg cgttggacgg
     7801 gctgatgttg atgcagccgt ggctcacgtt acgcaagccc tggtcgcctg ccgaccatgg
     7861 cgcgctgtgc acgaagttgc cggtgttgtc gaattgaacg gcgagctgga cgtgcagccg
     7921 gtaaccatcc ggcgatgtgt tcggcacgcc gtatgtcgcg gagtccatta cgacgtcgcg
     7981 catctttccc tggacgtagt aggtgccgtt gggagtgtcg tggccgggct tgcccatcga
     8041 catgggaaag acctgctcca catcgccgtt gcgggtgatg gtcagctgat gggttgcgtc
     8101 gtcagccgtg gcgaccaacg catcgccagt gacgaagctc gacttcgtac ctgctgcgtc
     8161 gacgttgacc acagcgtgcg cgggccagaa gtcgaatggc cgccagcgca cttgggaatc
     8221 gttcatccag taaaacttgc cggggacagg cggatccgac gaaacatgga tcgcttgttc
     8281 ggcggccgct cgatcagcga cggaagtgcc gaagtcgacg attatcggca tggcgacgcc
     8341 gaccattgag gtgtcggacg ggttgatcac cggcggatcg aacggcgcaa tgccggtgaa
     8401 cggagtcgga tcctgaccga ccggcgttcc atcggggatc gccaacggga gtggaagcgc
     8461 gaacacttcg atcggcgcag caggcggtgg gtcgggcggc gccacaacat cggcttccgg
     8521 atccgccagg gcgcgcggca tcaacccggc catggccgcc agcatcgcca ttgaaaccag
     8581 cactgcagcc cttcgggcac gcgaaggaca agaatcgtca cgcacccatg aatggttact
     8641 tgcctgtgcc acaaggccat caagcacgca taacggttag gcagcgaagt tcgatcccgg
     8701 cagccgtgtt tcgggcaaac ttggtgagca tgacgggtcg gacgagcgac aacctgctta
     8761 tcgtgcactg gcacgacacg ggccgctggc tcggcgccta tggacacccc ggcgtctcga
     8821 gcccgtgtgt ggatcgcttg gccgccgagg gcatccgctt cacgcgcgca catgcggcat
     8881 cccctggatg ttctcccgca cgcggcgcga tcttcacggg tcgttatccg cacagcaacg
     8941 gcctgatcgg gctggcgcat cacggatttg aataccgcga aggggtgcgc acgctaccgc
     9001 acattctgtc ggaagccggt tggtattccg cgctgttcgg tatgcagcat gagacgtcct
     9061 acccgtcgcg gctcggcttc gatgagttcg acgtgtcgaa ctcctgctgc gaatacgtgg
     9121 tcgaccgcgc gcaggaatgg ctcgtggaga gcgccccgct cgaggagggt gatccgtttc
     9181 tgttgaccgc gggcttcttc gaaacgcatc ggccgtggtc gcgagcgcgg tatgagcctg
     9241 cgccactcga cgatgtggaa gtgccggcat acctccctga tatcgcggaa gtcgacgccg
     9301 acatcgccga cttctacggg gcgatcgcgg tcgccgacgc cgccgtcggt cgtctgctcg
     9361 acacactggc cgagactggc tttgacgtca acacatgggt ggtgtttttc agcgaccacg
     9421 gtgagccgtt tcctcgagcg aaatcgatgc tgtacgaggc cggtacgggc atttcgttga
     9481 ttctccgccc gccgacgcgg ctgggaatcg caccgggtgt ctacgacgag ttgttcagcg
     9541 gcgtcgatct gctgccgacg ctgcttgact tgttgggcat cgagataccc gccgacatcg
     9601 agggcatctc ccatgccggg aatctgcttc ggccggccac cgacgccgca ccggtgcgcg
     9661 agcgggtgtt caccgagaag acctatcacg actcgttcga cccggcccgg gccgttcgca
     9721 ccaaggagta cagctatatc gagaactacg tgccacgcca tctcgtggat ctgccgctgg
     9781 acgtccagga cagcccgtcc gggaaagtgg tcgcgccatt cgtggcaggc ccgcggccgg
     9841 cgcgcgaact ttatgacctg cgcgaggacc ccgcggagtt gaacaatctg ctcgcaggtg
     9901 aagtgtccga gccagtagag cagatcgcca ccgagcttgc gattctcctt cacgagtggc
     9961 gggagcggac cggggatgtc tgtccatcgg agttcgccgg gcctcgaatc ctcgtcgagg
    10021 tcagtgagac gtacatcgag gccaccatgc gaaccctcgg cgtgcggccc acgtcgcagt
    10081 cgccgatggg gatccaccgt ggcttaaggg aattgaccgg cacgtcaggc taacggcgtc
    10141 ggacacggac cagggctcca cgtgacggcg cgatggccac cccgcgcgat tgccggcctt
    10201 cgtcgcgggc agtcgacggg tgaatggtgt agttgcgcca cagggttcgc aagactacgc
    10261 gtatctccat ggtcgcgaag gctgcaccga tacagcggcg aacaccgccg ccgtagggga
    10321 tccattccgc ggggtcgggg cgacgggaga tgaaccgatc gggatcgaag cggtcggggt
    10381 cggggaagac ggattcgtcg gcatgcagca gcccgatgct ggcgacgatc gtctgtccct
    10441 gcgggacggt ccacggaccg atccgcagcg tcggggcctt cacctgacgg aaggtggtat
    10501 cgatgaccgg gcgggtgcgc aggatctcaa agatggtggc gttcaacagt tcctcggatc
    10561 cggcgtcgag gtcggcgacg aggcggtcga gcaactgcgg gtgtcgctgc agccgctcga
    10621 cagcccacgc gagggtggtg gcggtcgtct catggccggc cgccagcatg gtgatcaact
    10681 cgtcggcgat ctccgcacga ctcatcggcg aaccgtcctc atagcgactc tgcaacatca
    10741 gggccagcac atcgtcccgc tcacccaacg cggaatcagc cagagccttg tcgatcagcc
    10801 ggtcgacaat cgcgtcgtac tcgcgacggt actggtagaa ccggccccat gggctccacc
    10861 ggccccaatc ccattgcggc acaggggcca acgcgagcat cgagccgagt ctgattcccg
    10921 gcggcagtat ctcgcggagg cgttccagct cgtgaccctc ggcgccgaac actgcgcgca
    10981 ggatcacgtt gagcgtgatc cgcatcatcg acggcatcgc cgcaaaggtc cgatcctcgg
    11041 gccaggttgc gaactcgtcg gccgcttcgc gttcgatgat cgcttcatag gcggccaggc
    11101 gccggccatt gaacgacggt gtgagcaatt tgcgctgggc gcgatgccgt tcgcccatca
    11161 gcgcgaacat cgaattcggc cccatcaccc ggccgagatt ggcgtcggtt gtgtccgcga
    11221 tgtccggggc ggtcctgaac agctgccgaa tgtggctggg atcgctgatg acaaccatct
    11281 gaccgaagat gggcaggtcg atcgtgaacg ccgatccgta tcggcgacgc aacatctgca
    11341 acgcgatcct gcggttggct accgctatca cgccttgcac ggcacgaggc agtggcggtc
    11401 ctggtggcat ctccacgacc gccgtcggtg cctcgcgctc aattgtggtc atggcgcggc
    11461 gacgatagcc cggcgaggca tcgagtcaat gcacggaaag tgtcgcttgt tacaactgtt
    11521 ggccgggcgt gcccgaggtc cgctccagga cgcgcatcga acccgttgcc aagacctcgc
    11581 ggaagtctcc cgtgctgagc gcacgctgat agatgtgaaa cggagcctgg ccgccgtcat
    11641 ttggatccgg gaacacgtcg tggatgatca acgcaccgcc gacatccacc cagcgggccc
    11701 agctgtcgaa gtcgcgctgt gcggcctctt cggtgtggcc gccgtcaatg aacaacagcc
    11761 gcaacggcat tcgccaaccg cgagcgacga caggggactt gccgacgatg gccaccacgt
    11821 agtcgtcaag gcccgccgtg tcaagcgtgt gccgcagcgt cggcagcgtg tcgaatagcc
    11881 cggtcacggc gtccaccatc gacgcgtcgt ggtactccca gcctgcctga tgctcttcgg
    11941 aaccgtggtg gtggtcgact gtgtagatca ccccgccgct ctgctgcgcc gcggccccca
    12001 gcagcacggt ggacttgccg cagtacgtgc cgatttcgac gccggcgccg tcacccagat
    12061 aacggacggc ggtgtcgaac agcgtgcggc cctcgtcggc gggcatgaag cccgtgacct
    12121 gctcggccag ggcgaagagt tcgtctgtgc ggctcatcgg cggctacctt accgttgctg
    12181 gtagatggtg gttgtagtag cgtccggaca cgtgtccgat ccggtcttgg agtcgactcg
    12241 gcgccgcctg accgccaaac aggccgacac tgtcgatcgc ctcggccgtg ccgcactgga
    12301 cctcttgagc cgcgaaggat ttgccggtct aaccgtgcgg cgggtagccg ccgaggccgg
    12361 agtcggggcg gcgaccgcct acacatactt ctcgtcgaag gagcatctcg tcgccgaggt
    12421 gttctggcgt cggctggccg cggccccacc cgcggcgcac gactcgggcg atgcggccgc
    12481 gcgcgttgtc gacgtgcttc gcgagatctc gctgttggtc gccgacgagc ccgagttcgc
    12541 ttgtgccgta acgaatgcgc tcctcggcaa agatccagac gtcgaggtgc tgcggctgcg
    12601 catcgggcgc gatatccgcg atcggctcgc cgccgcgctc gggccagata ctgatccgga
    12661 catcatcgac tcgctggaca tgctctattc cggagcgctg gtgcgcgcgg ggatgggcta
    12721 cgcgtcatac gcggatatcg cgcagaggtt ggagaagtcg gcgcggctga tgttgcgcta
    12781 gcgagggccg aggatcgcgg tcgcggccgc ggtggcgggc tcgactatgc cttgcacgtc
    12841 gcggtcgtcc tcgacaaaca gcgcggtcag ttcccgcaac ccgcccagca ggatcagcgc
    12901 gagcgggcgg gagatcggcg ccaggctcgc gcgccggaag ccggggctgt cggtgaggtc
    12961 gacgagcata tccgtgagcg cctccatggc gagccgatgc aacggcctcg ccaccgcccc
    13021 aagggcgggc gcctcgcgga tccagctcag cgtgatcgcc gggcgtgacc cgatgtggtc
    13081 gacgtaggcc ccggccgcct gacggatctg ttgctgccag tccgcctcgg ggtcgaccgc
    13141 cgcctggatg ctggcgatca ggtcctcgtt gtttctgcgc agcagctcga tcaggcactc
    13201 ttccttgctg gcgaattgct cgtagaaggt gcgcttcgac gtgcgtgcgt gccggacgat
    13261 gtcggcgacg gtcgtgtcgc gatagccgcg ctcaccgatc gatgcggcca gcccgtcgag
    13321 cagccggtct cggaaggcgt cgtcgaccga cgccgccttg tcgagcgctg tcgtcatttc
    13381 gcctccctga agcccttgcg cttatctggt accaagcggt accgtactac aaacacccac
    13441 ggtacaccga agtaccaggg aaacggccag gagtgattca cccatgactg agaccgcagt
    13501 gaccgccgaa gcaacaaccc cgacgactgt cggcaagatg ccgccggtcg tgcgcctgcc
    13561 gaaggccgta cagatggtcc tgatgtcggc ctttcgccgc ccgttcttgc aatacgcgtt
    13621 gaagcgttac ggccccgtct tcgccatcaa tgtcccgttc ttcggccgca gtgtggtcgt
    13681 gtccgatccc gcgctggccc ggcaggtgtt ccttgcgagt accgacgacc tgatcaacgt
    13741 gcagccgaac ctcagcagga tcttcggacc gggttcggta ttcgcattga acggcaagga
    13801 gcaccgggct cgccgcaagc tgctggcccc gccgttccac ggtcagagca tcaagaacta
    13861 cgaaaaggtc atcgaagagg agacgctgcg cgagacggcg atgtggccgg agggcgtgga
    13921 gttccggacg ctcgaaccga tgaacaggat cacgttgaat gtcatcctgc gcaccgtgtt
    13981 tggggccgag ggcgctgaac tcgacttcct gcgcgagatc attccgccct gggccaagct
    14041 ggggtcgcgg atggccaccc tgccggagcc gccgttcagc accgggcggc gcagcccgtg
    14101 gggccggttg gaggagttcc gccgcaactt cgaccgcacg gtcttcgcgc tgatccacaa
    14161 ggcccacgcc gacccgcacc tcgacgagcg caccgacatt ctggcgctgc tgctgcgtag
    14221 cacctacgag gacggcaccc cgatgtcgcg ccaggacgtc tcggacgaac tgctgacgct
    14281 gcttggcgcc gggcacgaga caactgcgtc gacgctgggt tgggccttcg agcggttgcg
    14341 caggcatcct gacgttctcg ccaagctggt cgacgagaac gacgacggcg gaaacgagtt
    14401 ccgccaggcc tccattcaag agctgcagcg gaaccggacc gtgatcgact tctccgggcg
    14461 gcatgttcag gcgccgcact tcgatctcgg cgactggcga attccccacg gctacacggt
    14521 gatgatcgcg atcgccaatg tgcacgccaa cgccgatgtc ttcccgaatc ccgagcgctt
    14581 cgacccggac cgcttcgtcg gcaccaggac accgacggcg tggatcccgt tcggtggcgg
    14641 cacccgccgc tgcatcggag ccgcgtttgc gaacgtcgag atggacgtgg tgctgcgaac
    14701 gatcttgcgg cacttcgtga ttgagaccga cgacgcccca gaggagaaga tccatttccg
    14761 gggcatcgcg ttcacaccga aggacggcgg gcgcatcgtc gtgcgccgcc gcgagtcagc
    14821 ggccgcctag gcttgctgtg tctcccgctt cggcagtttc cagcccggcc gcgggaagtg
    14881 gcaggtgtag cccgtcggga aacgctggag gtagtcctga tgctccggct cggcttgcca
    14941 gaagtcactc gccggggtga cctcggtgac caccttgccg ggccacagcc ctgacgcgtc
    15001 gacatcggcg atggtgtcca gcgcgatgcg cttctgctca tcgtcggcgt agaagatcgc
    15061 cgaccgatag ctggtgccga catcgttgcc ctgccggttg cgcgtcgtcg gatcgtggat
    15121 ctggaagaag aactccagca gattgcggaa gtccgtctgc gacgggtcat aggtgatttc
    15181 gatggcctcg gcgtggcccg ggtgattgcg gtaggtcgcg ttcgcgttct cgccgcccgt
    15241 gtagccgacg cgagtcgata cgacgccggg ctgcttgcgg atcagctcct ccatgcccca
    15301 gaagcagccg ccggccagga tcgccttctt gtattcagtc acgctgattc ctcctttgag
    15361 gccgacttgc tgaacaaagc cttgtaatcg ccatagccct ctgcctcgag atcgtcgagg
    15421 tggatgaatc tcagcgaagc cgagttgatg cagtagcgca acccaccctc ggcccgtggt
    15481 ccgtcggtga agacgtgacc gagatggctg tcgccgtgtg cggagcggac ctcggtgcgg
    15541 atcatcaggt gggagaagtc gcgcttttcg acgacgttct cggcatcgat cggtttggtg
    15601 aagctgggcc aacccgtgcc gctgtcgaac ttgtcgaccg aggcgaacaa cggctcgccc
    15661 gacaccacgt cgacgtagat gcccgggtcg tggttgtccc agtactcgcc ggtgaagggg
    15721 cgttcggtgc cgttcttctg cgtcacgttg tactgctctg gcgagagcgc gccgaccgcc
    15781 gcaggatttt tgttgtattg atgcgacata gtgcctttat aaccgcgtgg cgtgcctgat
    15841 taatccgcgg ctgtcgctac cgtaaataca tgcggtcagt cgttctcgct gtctgcgtgg
    15901 tgctcgccgc gtggagtgcc ccgtcagcgt cggccgaccc ggtcgcgcca cccgaggcaa
    15961 ccgatgccga aggcgtcgct ttcacggaca acccggcgat cgtcgatccg catccgatgc
    16021 cggtcgagtc gtggagtcgg gtcggcaacg atcgcgccgt ggcggtgcat ttcacgaccg
    16081 gcacacccgc ctgtaacggc gtgcatgcga ccgctcacga gacggccgac acggtcacga
    16141 tcgacctgaa aggtggttcg ttgcccgcgt ccgccggccg catgtgcatc atgcttgcgg
    16201 tgaccggcac gctcgtcgtg ccgctgcaaa gcccgctcgg cgaccgacag gtgctcagcg
    16261 cggcataggc cccaagcgga ccttctccac gatcgcgacg atcttgtcgg cgacaacttg
    16321 gggctgataa agcatcacgt cgtgcccgct gccggcgacg atcaggttgt ccgcccagag
    16381 ggtctcggcc agcacgctgt tcgctcggtg aacctggaac ttggtgtagc tctggggcgt
    16441 caggtcagtg ggcggcggga acttgtcgga actgagcacg acagcgggca ccctcggcag
    16501 cggtggcgca ggcgccgcct tgagccgagc gaacgcgtcc gcggcgagaa acccttcacc
    16561 gtgcgcattc ggcgccggtg cccgtgcctc ccggtcgaac tcggtgtcct gatcgggtct
    16621 gcccagcctc ggtaagtact ccgacgtcgg atccacgaac acgagaccgc tgacgaactc
    16681 ggggtgggtc cgcgatagca ggtcggcgat gagcgcgcca tacgaatgtg acaccacgac
    16741 catcggtgtt gaaaggtccg cggcgacaat cagtttcacg atgtcgttga cgtcctgctg
    16801 gactgtgtgc ggttgaggga caggggtgga ccgctctggt ccgtcgggtc gcgtgttggg
    16861 ccggtcgtag gcgcagacgc gggtggtctt cgcgaggacg ggcaccacga cgttccactc
    16921 cgcggcgtaa cttcccttgc cgggaatgat gaaggccgtc ggcgatccgg tgccctgaca
    16981 gttcagaaag agttggcggc cattgccgat gtacaccagc cccgcgaagt cgggttcatg
    17041 cggattcggc gcgcatccgg cgaaaacagc gagcgcgaag gcaagcccga gcagggcctt
    17101 cacgtgtctg acgatacgac cgacgctcga caaacgtggg tagaacgtgt tctagttctg
    17161 gttgtgacga gcaagacgtt cacccgcgac gagctggccg cggcgttcga gacattcgag
    17221 gcgacggtcg atcgggccgc gcagagccgc gactgggatc cctgggttga gcagtacaca
    17281 cccgacgtga cgtacgttga acacgcagcg ggcaccatgc acggtcgcga cgaggtgcgg
    17341 gcctggatct ggaagaccat ggagaccttt cccggcagct acatgacgtc gttcccgtcg
    17401 ctgtggtcgg tcatcgacga gccgaccggc cggatcatct gcgagctgga caacccgatg
    17461 cgcgacccgg gcgacggcac catcatcagc gcctccaaca tctcgatcct gacctacgcc
    17521 ggcgacggcc actggtgccg tcaggaggac atctacaacc cgctgcggtt cgtctcggcg
    17581 acaaggaagt ggtgccgcaa agcgcaggaa ctgggcacgc ttcccgacga ggcggcggcc
    17641 tggctgcgtc agttcggaga cctgaggtga gcgcgaaact tgtcatcggg gccaacggct
    17701 tcctcggctc gcatgtcacc cgacagttga tagccgacgg tcatgaggtc cgggcgatgg
    17761 tgcggccgaa cgcgatgaca gttggcatcg acgacctgcc cgtcacccga ttccacgggg
    17821 acatctggga caacgacact ctgcgcgagg cgatgaccgg tgtcgacgac gtgtactact
    17881 gcgtggtcga cgcccgtggt tggctgcgtg atcccgcgcc gctgtttcgc acgaacgtcg
    17941 acggcacgcg caacgtactg gagatcgcga aagacgctgg gctgcatcgg ttcatctaca
    18001 ccagcagcta cgtcaccgtc gggcgccgcc gcggccacat tgccagcgag gacgacacca
    18061 tcgtcgaccg cgggctgacg ccgtacgtcc ggtctcgggc gcaggccgag aacatcgtgc
    18121 tcaagtacgc gacgcagcac ggcttgcccg ccgtcgcgat gtgcgtgtcg accacgtacg
    18181 gaagcggtga ctggggccgc accccgcacg gcgcgatcat cgcgggcgcg gcattcggca
    18241 agctgccgtt cgtgatgagc ggcatcgagc tggaggccgt cggcgtcgac gacgcggcgc
    18301 gcgccatgat cctggccgcc gagcgcggcc gccaggggga gcggtacctg atctcggaca
    18361 agatgatcag taatgccgac gttgtgcgca tcgcggcgga ggcggcggga gttccggctc
    18421 ccaccaagtc aattccacta cctgtgtcgt acgcattggc gaccgcgggc agcttcaagg
    18481 ccaagctgcg cgggaccgac gaacggctgt cgctggggtc gttgcggttg atgcgtgctg
    18541 aggcgccggt cgaccatggc aaggcggtac gcgaattagg ttgggagcca agaccagtgg
    18601 aggaatcgat ccgcgaggcc gcccggttct gggtgggcct gcgcgacgcc aaacgaaaga
    18661 gcaagacggc caattagccg gagatcggca aattgacctc cgcgtcagtc gggttggtct
    18721 acagtcgcgc cgtgggggaa gaccaagcgc ccactgatgc acccggcacg gagcccgggg
    18781 gcaatcccag cggtgccaag atcggtgagg cgctcgcgtc cgagctgtca cgcgtcgtcg
    18841 ccgcctacac ccaacagatt caggaagcgc tgcaaacctg cctttctcag ctcaccgagc
    18901 agtggcagtc ggcgtttgcg gtgtacgccg ccgagtacac caagcaatgg caggaggcca
    18961 tgcgggcggt ggccgtgcag cagacgcatg gtgctccggc ctccgaatca ttcgaggaga
    19021 agcaggggga ggccgaatag acatgcgcgc cgaggtttct gcatcgcttt gtagtccaga
    19081 ggatcgtccc gtcagcctga agatcgatcc ggtgaaactg gatgcgcggt catctggaac
    19141 accgacggtc agcgccgacc ccatcgaagt cgagctgccc gacatcaccg tcacagtccc
    19201 cgaaatcacc gtcgagttgc ccaatcccac gctcaacgtg acctttgggg ttggcgacag
    19261 cggcgtcgtc gaatacctcg agggtgttcg ggcgttcttg caatcgctgg accctttgga
    19321 tcccagtgac gtcgccttgg gggccgccgc gctgttggcg atcgggcccg acctgattcg
    19381 ggcggttgcc ctgccgaaac tggagctcga ggccgagctc gagttcgggg aactgaagat
    19441 atcggcggac gaggcgctcg ccatcgacac cggccgcatt cgcctgcacg gcgtgccggc
    19501 caccgatgac gtgctgccag tcaagatcgc cgcagacctg gacgaaaccg gcgtgactgc
    19561 actcctcgac ggcatgcggg cgaccctgac cggttgcctc gtgctgaacg gtggcgaccc
    19621 accgccggca cctccggccc cgcccgccac cgtgcagggc gttgacgtga aggtcgacgc
    19681 gaacaagaag ctgctggttc tcatcatcca cggccaggga ttcgggccgg tgcagggaac
    19741 ggtggtgctc agtcaggtcg ggctgtcgct gacgccggtc caattcgttt cctggaccga
    19801 ccaggagatc gaggtgatct tccaaccgat cccgccgccg ggggcgtatg cggtcaccgt
    19861 cacgaccgcg cagggggcgt cgacgcagcc gctgccagtc accgtgagca attagccgct
    19921 gattcgaaac agcgccatcg cgttggcgtc cagaatggca tgggcctccg cgggatcggt
    19981 ggcgtcgatg atgtactcga cggcacggac gaatgtgtcg ccgtcctcgt agggaaaatc
    20041 ggtgcccagc aggatgcggt ctgcgccgaa tgagtcgatg gcgcaacgca atgccggtgc
    20101 gtggcagtgg ctcacggtgt cgtaccacag ccggtgaacg gcatgactgg gcgcctcggg
    20161 cgtatccggc gcctcccatg caacatgatc gtccgcgcgc agcgggatca tcggcaacgc
    20221 gccgccgagg tgcgaacaga tgatccgcac gttggggaaa cgctgcaggt gacccgacgt
    20281 gatcagctgc atcgcggcga tggtgtcctc aaaaggtgcg ccgactgtcc acgtgatgtg
    20341 atgctcgctg accagcggcg aacacgcccc gttgccgacg gggtgcagat acagaatcgc
    20401 gccgcgccgg tcgagttcgg cgaagacggg ctcgaactcg ggatcggtga tcgctcgatc
    20461 gagaatgctg gtgttcatcg tcagcccgga catgcccaat tcatcaatgg ccctagacat
    20521 ttcggtgatc gaagcgtcga tgtggggcat cggcgtggcc gcgaacgcgc gaaagcgatc
    20581 aggatgggac gacaccagcg ctgcgtactc gtcgttgaca tagcgtgccg cggccgcggt
    20641 acgggccacg tcgtcgccgt atggcaattg cggcgcggcg gacagcactt ggatgtcgac
    20701 gcctgcgcga tccatcaacc gcagccgcgc atccagttcg gccccaccgc cggctccgat
    20761 gccgcgttgg gtggcggtgt cggatctgcc gaggtcgacc agcatgtcga ggtacgcgtc
    20821 ggtccagtag tgcgcatgca cgtcgatacg catgtctgtc aacggtattc ggttgaacgc
    20881 cacgccgacg ggtgtttagt gaatagcctc atccatcgcg gtcggactag cgtcggatga
    20941 cgtgaccgag aaactgcacg tcgacctgtc cggtgcgccg cagaccatgc tggccacgtt
    21001 ctacgcgaag gctgtggacg ccgaccttcc ccagccgatc ctgggtgacc ggtttgcgaa
    21061 ggacattgtc gaccgcgtcg actacgactg gaaaaagacg tcgatcacgg ccacgaactc
    21121 gccggcggtg acgacccgct ccgcgcactt cgacaactgg gcgcgtcagt tcctcgcagt
    21181 ccacccgcac gccgtcgtgc ttcacctggg ctgcggtctg gacgcccgct tcttccggct
    21241 ggccccaggc ccgggtgtgg attggtacga catcgactac cccgacgtcg cgcacctgcg
    21301 tcagcagctc tatccgaccc gcgaccacta ccgcgtcatc gcggcctcgg tcaccgaccc
    21361 gagctggttc gccgagattc cgcgagaccg cccgacgctg atggtcggcg agggtctgac
    21421 catgtacctg actcaggagg acggcatcgc gctgctgcgt cgcgtcgtgg agcacgctcc
    21481 gtccggcgaa ttgcagttcg acgcgttcaa ccggttgggc atcaaggcgc agtggaccaa
    21541 cacggtggtg cggcgctcgg gagcgacgct gcattggggc atcgacggac ctgacgacat
    21601 cgtcacagcg gtgcccgggg tgcggctgct gtcatgggtc tcggcgatcg acggagacgc
    21661 gttcgcgcgg gtgccctggt actaccggtg gacgctgaaa gcgatgagga tcgcgccggt
    21721 gctgcgctac atggcgcagt accaccgcta cgccttctga gcgcggacgc ccagcaccgg
    21781 ttgtcgacgc cgaccgtggc cccaacccgc ggcccatggc cagcggccgc ggtcgtctgc
    21841 ccacaccaac tgcagcgctc gaatttccgg gccgccaagc gccacggcga aattcatgtg
    21901 cgcatcggga tggtccactt cgacgatctc gaggagcggg ccgtcaccca cagcgatgtg
    21961 cgcgccgggt tggaaaacgc gaccgccgac cgcggcgacc gcgacatcgt tcagcacgcg
    22021 tgccgccctc tcctgcgata ggccggtcac aagaagttcc ggcagaccgc ggtcgtgtag
    22081 gccgattgtg taggcgtacg gaagtcgatc gtcctcgacg ccctgcaccg cccacccatg
    22141 aatccggatc agcgcgcgga gttcgtcgag gtattcctcg gtggtgccgt tggggttgtc
    22201 gcactgccag cacatgaccg cacctttctg tcgtcgttac ggccagcttg cgccgggggt
    22261 ccgacaaccc agcaccggtt gcatcgctcg tccgtcagcg aactccgctg accatggcca
    22321 gcgaccacgg ccatcggccc aaaccaactg cagcgcgcga atctccggcc cgccgaccgc
    22381 caccgcccag cccatgtgcg cgtccgggtg gtcaacctcg acgatctcga tgagcgggcc
    22441 atcgggcagc gaggtctgtt gtcccggcgc gaacacatcg ccgcccacca accttcgcgc
    22501 gacgatgtcc agcagccgga gcgctcgctg cggtgatacg ccggtcatga gcaactcagg
    22561 cacatcccag tcatgcagcc cgatcgtgta tgtgtaaggc ctacggtcgc tttcgacgta
    22621 ctgcaccgtc caacccttct tgcggatctt tcgttgcaac aggtcgcagt agtcgtcgtg
    22681 cgtcgcttcg ggtgatcgca caaccagcac atggcacgcc tctctctgtc gtcgttgcgg
    22741 ccagcctgca ccggtggtcc gacaagtcca cgtcttgcac ggagagctcg cgggtagccg
    22801 atcaacgaaa ggaggcttca tgctcgtcga ccacccgatg acgctgacca tccccgtcag
    22861 cgacttggag cgagcccgag cctggtaccg cgacaagctc ggcttcaatc ctgttcacga
    22921 ggtaccgggc gaggcactgg cgtaccgcac cggctccggc cagtcactgc tgctgttttc
    22981 atccgacgag gccggagctg tcgagcatca gctcgcggcc tggcgcgtcg atgacgtcga
    23041 ggcggaggtg acgagctagc gggcttcggc cttccccttc agccgctgca gcgtcagctt
    23101 gatgtgttcg gcgttggccg cggtgcggtc cttcaccccg gtcgtgaact gcgcaggttt
    23161 acggatccaa ccgggacggc ggtcccaggt gcgctcggtc accttgcagc cgccctcgac
    23221 cggttcgatg tcgtagcgcc agcgggccac gggcacgacg gcggccgtct tgacgtcaaa
    23281 ggcgaaggcc ctgcctggct gcgcgtcggt gacggtgcat acggtggtcc acctgcgtga
    23341 gccgttgcgg ttgtatccct tgaacacagc accgggacgc accgcgtcgc ccttgcgcca
    23401 cttcatcgcc tccgcctcct cggcgcacgc ggccagcgtc ggaaggtcgg taatgagggc
    23461 gtacaccgcc tgcggatcag cgtcgatggt cacatctgcc tgtgcagagg ccggctcctg
    23521 ctcagtcatg gcctgatcgt atcgacggat tccggaacag gggccgttgt gcaggcgttg
    23581 cctcatctat gagcgaccga cccgtactcg aacccaccgc ttcccatccc atcaccgtcg
    23641 aacccaccgg aaaacacgtc accgtgcgca tcaacggcga actggtggcc gacaccgaca
    23701 acgcgctgac cctgcaggag tccacctatc ccgcggtgca gtacgtcccg ttgggcgacg
    23761 tcgccgctga gcggctgcga cccagcgaca ccgagacgta ctgcccgtac aaaggcgacg
    23821 cgagctacta ccacgtcgtc accgaagccg gcgggaccgt cgacgatgcc atctggacct
    23881 acgagaagcc ctatccggcg gtcggccaga ttgccggaca cgtcgcgttc tatccggaca
    23941 aggcggagat ctcagtcgca tagcctgatg cggtgcatcg ttcggtcagt gtcatcttcg
    24001 acgggccggc cagccccggc ttgaccctgt cgccgctgag gcgcggccga ggcgacccgt
    24061 gctatcacga cgcgcaagac ggtgcgatct ggcgcaccag cctgatgcgc agcggacccg
    24121 tcacggcgcg aatcacgaag tcggcgcccg ataccgtcga ctgcgaggcc tggggaagcg
    24181 gcgcaacgga attcaccgaa accttgcctg cgctactcgg ggccgacgat gacgcgtccg
    24241 gcttcgctcc cgacgagccg acgatcgccg ccgcgcatca acggatgccg cacctgcggc
    24301 ttggccgcac cgaccgggtg atcgagtcgc tcatccccgc gatactcgaa cagcgggtgt
    24361 acggcatgga cgcgcgccgc gcgtggcgag cgctggtgac caagttcggg tcgtccgccc
    24421 cgggccccgc gcctgagcac atgcgggtgc cgccggccgc ggaggtgtgg cggcgcattc
    24481 cgtcgtggga gtttcaccgt gccaacgtcg acccgggccg ggcccgcacg atcgtcggct
    24541 gtgctcagcg cgccgattcg ctggagcggc tggccacccg gccgcccgag caagcgcggg
    24601 cggcgatgat gtcgctgcca ggcgtgggcg agtggaccgc ggccgagacc gcgcagcggg
    24661 cgttcggcga cgccgacgcg ttgagcgtcg gcgactacca cctggcctcg atggtcgggt
    24721 ggcggcttct cggggcgccc atcgacgatc cggcgatggt ggagctgctc gagccgttgc
    24781 ggccgcaccg ccaccgggcg gtccggttac tggaggtcac cggccctgcg cacgctccgc
    24841 ggagagcggc gcgtcaggcg attccaaacc ttcgtgcgct ctgaccacga ttactgacct
    24901 gggcgcgtgg gtatgcgtcg cgcatgactg agttcaccat tcccggaata tccgagaagg
    24961 acgctaacga ggttgccgac atcctgcaga aacagctgag cacgtacaac gatctgcatc
    25021 tgacgctcaa gcacgtgcac tggaacgtgg tcgggccgaa cttcatcggc gtccacgaga
    25081 tgatcgaccc ccaggtcgaa acggtccgcg catatgccga cgtgtcggcg gagcgcattg
    25141 cgacgcttgg caagtcaccg cagggtacgc ccggcgcgat catcaaggac cgcacgtggg
    25201 acgactactc cgtcggccgc gataccgtgc aagcgcacct ggcagcgctc gaccttgtgt
    25261 acaccggcgt catcgaggac acccgcaaag ccattcaccg catcggcgag cttgactccg
    25321 tgaccgagaa catcctcgag gaacacgccg ccgagctgga aaagttccag tggttcgtgc
    25381 gcgcgcatct cgagaacgcc ggcggccagt tggccagcgc gggtgccaag acggagcggg
    25441 ccgccgccag caaggccaag aagaaggcgc cggtcaagta gtagcgggtt cctccagagc
    25501 gtcgtcacgc accgggcggt cggcgagttc ctcgttgcgg ccgaacaggt atgggtaggc
    25561 gatgcccgcg atggctgcgc ccagtagcgg tgccaaccag aac
//
LOCUS       DASRJA010000107         5457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2450667,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000107 DASRJA010000000
VERSION     DASRJA010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2450667"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFC01_05015"
     CDS             <1..417
                     /locus_tag="VFC01_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HZN79041.1"
                     /translation="RKEPSLTLHLPDREPVSGVHFAFASNSSPWTYANARPVWTNPTT
                     TFETGLGVFATTSTNVWANLGLVRQMVSKEPRIEAKHLIRDDDVAWVRVTSDTPVACQ
                     IDGDYLGTRSEMTFTAVPDALGVLAPPAKVPSDLHK"
     gene            652..906
                     /gene="whiB1"
                     /locus_tag="VFC01_05020"
     CDS             652..906
                     /gene="whiB1"
                     /locus_tag="VFC01_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator WhiB1"
                     /protein_id="HZN79042.1"
                     /translation="MDWRHKAVCRDEDPELFFPVGNSGPALAQIADAKLVCNRCPVTT
                     ECLTWALDSGQDAGVWGGMSEDERRALKRRNARTKARSGV"
     gene            complement(1043..2560)
                     /locus_tag="VFC01_05025"
     CDS             complement(1043..2560)
                     /locus_tag="VFC01_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HZN79043.1"
                     /translation="MSTLGDLLAEHTVLPGNAVDHLHAVVGEWQLLADLSFADYLMWV
                     RRDDGVLVCVAQVRPNTAPTVLLADAVGSLADSDHLPLVTAAFESGTIGRENGGGQGD
                     SRTANVAAAAASDLKVEAVPVRYKDEVVAVLTHQTAIAARKASPLEIAYLDCAGDLLL
                     MLSEGTFPNVGDLAMSRSSPRVGDGFIRLDEAGNVVFASPNAISAYHRMGLTAELEGH
                     NLVAITRPLIGDPFEAQELANHVRDSLAGGSSMRMEVDAGGAAVLLRTLPLVVHGKAV
                     GAAVLIRDVTEVKRRDRALLSKDATIREIHHRVKNNLQTVAALLRLQARRTNNAEGRE
                     ALIESVRRVSSIALVHDALSMSVDEEVNLDEVVDRILPIMNDVATVDTPIRINRVGDL
                     GVLDADRATALIMVITELVQNAIEHAFDGTKQQGCVTIRAERSARWLDVVVHDDGRGL
                     PDGFSLEKSDRLGLQIVRTLVSAELDGSLGMHDVSTGGTDVVLRVPIGRKARLAQ"
     gene            complement(2587..2802)
                     /locus_tag="VFC01_05030"
     CDS             complement(2587..2802)
                     /locus_tag="VFC01_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HZN79044.1"
                     /translation="MAEDVRAEIVASVLEVVVHEGDQIGEGDTLVLLESMKMEIPVLA
                     EVAGTVSKVSVSVGDVIQAGDLIAVIS"
     gene            complement(3034..3330)
                     /gene="rsrA"
                     /locus_tag="VFC01_05035"
     CDS             complement(3034..3330)
                     /gene="rsrA"
                     /locus_tag="VFC01_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HZN79045.1"
                     /translation="MSDDERWSAPIGPVDPEHPECAAVIAEVWTLLDGECTAETRDKL
                     KHHLEECPTCLRYYGVEERVKTLIAKKCSGEKAPDGLRERLRIQISRTTIIRGG"
     gene            complement(3327..3983)
                     /locus_tag="VFC01_05040"
     CDS             complement(3327..3983)
                     /locus_tag="VFC01_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN79046.1"
                     /translation="MDGTAPDTAPESSGSDRAEETDAELTARFERDAIPLLDQLYGGA
                     LRMTRNPADAEDLLQETMVKAYSGFRSFREGTNLKAWLYRILTNTYINSYRKKQRQPS
                     EYPTEEITDWQLAANAEHSSTGLRSAEVEALEALPDTEIKAALQALPEEFRMAVYYAD
                     VEGFPYKEIAEIMDTPIGTVMSRLHRGRRQLRELLADVARDRGFIRGQQLDAPEEVSS
                     "
     gene            4192..4709
                     /locus_tag="VFC01_05045"
                     /pseudo
     CDS             4192..4709
                     /locus_tag="VFC01_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
     gene            4719..>5457
                     /locus_tag="VFC01_05050"
     CDS             4719..>5457
                     /locus_tag="VFC01_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN79047.1"
                     /translation="MSLSGKTMFISGASRGIGLAIAKRAAADGANIALIAKTAEPHPK
                     LPGTVYTAAKELEEAGGQALPIVGDIRDEESVAGAVAQTVEQFGGIDICVNNASAINL
                     GSIKEVPLKRFDLMNGIQVRGTYTVSQACIPHMIGRENPHILTLSPPVRLEPEWLKPT
                     AYMMAKYGMSLCALAMAEELREDGIASNTLWPRTLVATAAVQNLLGGDEAMARARTPD
                     IYADAAYQVLIRDPRTCNGNTFIDDEVL"
BASE COUNT          966 a         1795 c         1759 g          937 t
ORIGIN      
        1 cgcaaggagc cgtcgctgac gctgcacctg cccgaccgcg agcccgtttc cggtgttcat
       61 ttcgcgttcg cgtcgaactc gagcccgtgg acgtacgcca acgcccgccc ggtgtggacg
      121 aaccccacca ccacgttcga gaccggcctc ggcgtgttcg cgacgaccag cacgaacgta
      181 tgggccaatt tggggctagt gcgacagatg gtgtcgaagg agccgcggat cgaagccaag
      241 cacctgatcc gcgacgacga cgtggcgtgg gtgcgggtga cgagcgacac gccggtggcg
      301 tgccagatcg acggggacta cctgggtacg cggagcgaga tgacgttcac ggcggttccg
      361 gatgcgctgg gcgtgctggc tccacccgca aaagtgcctt ctgacctgca taaatgatat
      421 tcacagggcc gattcagggc gcagactggt taagtgtagt acaaacgaat gagggttctg
      481 ccaacgaccc tcgttagtga gattacccac ttgttaactc gcgagtattg acatctgtcc
      541 agcctgtgaa agcatcggat gcaacagtgc agaaacattc tcaatgcacg cgttaacagc
      601 cgaagaaaag ctcgtgcgcc ctgctgcgca cacagtaagg agatcaggac tatggattgg
      661 cgccacaagg cggtctgtcg cgacgaggat ccggagctgt tcttcccggt agggaacagc
      721 gggccggcgc ttgcgcagat cgctgacgcg aagctggtct gtaaccgctg cccggtgacg
      781 acagagtgcc tcacctgggc attggattca ggccaggacg caggcgtgtg gggcggcatg
      841 agcgaagacg aacggcgcgc gctgaagcgg cgcaacgccc gcaccaaggc ccgtagcggg
      901 gtctaaccgc cacccagcgt ttcaagtttg gctacggccc cggcaaattc tgccggggcc
      961 gtaactgtgt gcaaatgcaa tttgatacga attgctgggc gaatcgtcaa tttgagaaat
     1021 cgatcacatt tcggctgtgc gtttactggg cgaggcgcgc cttgcggccg atcggcaccc
     1081 gcagcacgac atcggtccca ccggtggata cgtcatgcat gccgagtgaa ccgtccagct
     1141 ccgccgacac cagcgtccgg acgatctgca ggccgagccg gtctgacttc tccaagctga
     1201 aaccgtccgg cagtccgcgg ccgtcgtcgt ggacgacgac gtcgagccag cgcgccgagc
     1261 gctccgcgcg gatcgtcaca caaccctgct gcttggtgcc gtcgaaggca tgctcgatgg
     1321 cgttctgcac aagttcggtg atcaccatga tcaacgcggt ggcgcggtcg gcatcgagca
     1381 cgccgaggtc gccgacccgg ttgatgcgga tcggagtgtc cactgtcgca acgtcattca
     1441 tgatcggcag aatccggtcg accacttcgt ccaggttgac ctcttcgtcc accgacatcg
     1501 acagcgcgtc gtgcaccaag gcgatcgacg acacccggcg tacggactcg atgagcgcct
     1561 cgcggccctc ggcgttgttg gtgcgccgcg cctgcaggcg cagcagcgcg gcgacagtct
     1621 gcaggttgtt cttcacgcga tggtgaattt cgcggatggt ggcgtctttg gacagcagcg
     1681 cccggtcgcg gcgcttgacc tccgtgacgt cgcggatcaa gaccgccgca ccaacggcct
     1741 tgccgtgcac caccaatggc agcgtgcgca gcagcaccgc cgctcccccg gcgtcgacct
     1801 ccatgcgcat gctcgatccg cctgcgagcg agtcacggac gtggttggcc agttcctgcg
     1861 cctcgaacgg gtcgccgatc aacggccggg tgatggcgac caggttgtgg ccttcgagtt
     1921 cggcggtcag ccccatccgg tgataggccg agattgcgtt cgggctggcg aataccacgt
     1981 tgcccgcctc gtcgagccgg atgaacccgt cgccgacgcg cgggctggac cgcgacatcg
     2041 ccaagtcacc gacattgggg aaggtgcctt cggacagcat cagcaacagg tcaccggcgc
     2101 agtcgaggta ggcgatctcc agagggctgg ccttgcgcgc ggcaatcgcc gtctgatgcg
     2161 tcagcaccgc cactacttcg tctttgtagc gcaccggcac ggcttccacc ttgaggtccg
     2221 aagcagccgc ggcggcgaca ttagcagtgc gcgaatcgcc ttgtccccca ccattttcgc
     2281 ggccgattgt gccggactcg aaggcagccg tcaccaacgg cagatggtcg gaatcggcga
     2341 gcgagccgac ggcgtcggcg agcagcacgg tcggcgcggt gttcggccgg acctgtgcga
     2401 cgcacaccag tacgccgtcg tcgcggcgca cccacatcag atagtcggcg aacgacaggt
     2461 cggccagcag ttgccactcg ccgaccacgg cgtgcagatg gtcgacggcg ttgccgggca
     2521 gcaccgtgtg ctcggcgagc aggtcaccga gggtcgacat cggggcttac tccggcgtgg
     2581 aggcggtcag ctgatcaccg cgatgaggtc gccggcctgg atgacgtcgc caacagacac
     2641 gctcaccttg ctgacggtgc ctgcgacctc cgccagcacc ggaatctcca tcttcatcga
     2701 ctccaacagc accagggtgt cgccctcgcc gatctgatcg ccctcgtgaa ccacgacttc
     2761 aagcacgctg gctacgatct cggcgcgaac atcctcggcc atcttcaccc ctttcggctg
     2821 ctgtgtgccg ctctatcgaa ccacaacgtg gggtgggggg tgctctggca ggccatgaga
     2881 caatggactc aacgcgcccg ccactggcgg gttagacaac cccctgacca gacggaggaa
     2941 aaccatggcc aagcgtggcc gcaagaagcg cgatcgtaag cattccaagg ccaaccacgg
     3001 caagcggcca aacgccggtt cgaaatcggt gcgctagccg ccgcggatga tcgtggtgcg
     3061 gctgatctgg atccgcagcc gctcacgcag cccatcgggg gccttctcgc cgctgcactt
     3121 cttggcgatc agcgtcttga cccgctcctc gacgccgtag taccgcaggc aggtggggca
     3181 ttcttcgaga tggtgcttga gcttgtcacg cgtctctgcc gtgcattcac cgtccagcag
     3241 cgtccatacc tcggcaatca ccgctgcgca ctcgggatgc tcggggtcga caggcccgat
     3301 cggggcggac cagcgttcgt catcgctcat gaggacacct cctcgggcgc gtcgagctgc
     3361 tgtccacgaa tgaagccgcg gtcccttgcc acatcggcca atagctcgcg cagttggcgc
     3421 ctgccgcggt gcagccggga catcacggtc ccaatcggcg tgtccatgat ctcggcgatc
     3481 tccttgtagg ggaagccttc gacgtcggcg tagtacaccg ccatccggaa ctcctcgggc
     3541 agtgcttgca gggcggcctt gatctcagtg tccggcagcg cctcgagcgc ctccacctcg
     3601 gccgaccgca gacccgtcga gctgtgctcg gcgttggcgg ccagctgcca gtcggtgatc
     3661 tcctcggtcg gatactccga cggctggcgc tgcttcttgc ggtagctgtt gatgtaggtg
     3721 ttggtcaaaa tccggtacag ccacgccttc aggttggttc cctcgcggaa cgagcggaag
     3781 ccggagtagg ccttcaccat ggtttcctgc agcaaatcct cggcatcggc ggggttgcgc
     3841 gtcatccgca gcgcaccgcc gtagagctgg tcgagcagcg gaatcgcgtc gcgctcgaac
     3901 cgcgcggtca attcggcgtc ggtttcctcg gccctgtcgg accccgacga ctctggcgcc
     3961 gtatcgggcg ccgtcccgtc gatttcggtc actgtgggaa acaccgtccc ttccgttgcg
     4021 gcgccaccaa acaggcccgg tatgtccacc ggacggtcca ggcaaatggt aggcaccaaa
     4081 gaccccttcc ttcgatccta gaggcagtaa ccgacatagc ccgttcacgg gctgaactgc
     4141 atggcataac agcacggacg ggcgcggttg ttcccgcatt acgctgagcc ggtggcgcgc
     4201 gcggcaaccc cggcgatcgc ggccctgtcc ccctcgcaag ctgggggcac ccccaggccg
     4261 cggaagtccc gcatcaggtg ctgcagtaca cgcacgaccc gcgcaacgag tcgttcggcg
     4321 aggaagcggt cgaggagctt gcgcaggtcg agggcgtgga tccggcgcag gtgttcaaga
     4381 ccctcgtcat cgcgctgccc aagggtctgg ccgtcgccgt catccccgtg ccgtcgaagt
     4441 tgtcactgaa ggccgccgct gcggctctgg gcgtgccgaa ggtgacgatg gctgaacggg
     4501 cggcggcgga gcggtccacc ggctacgtca tcggcggtat ttcaccgttc ggtcagcggc
     4561 ggccgctgcc caccgtcgtc gatgcctcgg ccctgcactg ggaccgggtg ctgtgcagtg
     4621 ccgacaagcg gggctgggac gtcgccgtgc acccgcagga cctgatcggg ctgacgaacg
     4681 cggtcaccgc cgatatccgc gcgtcctaag gtgaccccat gtcgctttca gggaagacca
     4741 tgttcatttc cggcgccagc cgtggcatcg ggttggcgat cgccaagcgt gccgccgccg
     4801 acggcgccaa catcgcgctg atcgccaaga ccgccgaacc gcatcccaag ctgccgggca
     4861 cggtatacac cgcggccaag gaactcgagg aggccggcgg tcaggcgctg ccgatcgtcg
     4921 gcgacatccg cgacgaggaa tcggtcgcag gggcggtggc gcagaccgtc gagcagttcg
     4981 gcggcatcga catctgcgtg aacaatgcgt cggcgatcaa cctcggttcg atcaaagagg
     5041 tcccgctcaa gcgcttcgac ttgatgaacg gcatccaggt gcgcggtacg tacaccgtgt
     5101 cgcaggcgtg catcccgcac atgatcggcc gggagaaccc gcatatcctc acgctgtcac
     5161 cgccggtgcg tttagaaccg gagtggctca agccgaccgc gtacatgatg gccaagtacg
     5221 gaatgtcatt gtgcgccttg gcgatggccg aggaactgcg cgaggacggc atcgcttcca
     5281 acacgctgtg gccccgaacg ctggtggcaa ccgccgcggt gcagaacctg ctcggcggtg
     5341 acgaggcgat ggcgcgcgcc cgcacccccg acatctacgc cgacgccgcc tatcaggtgc
     5401 tgatccgcga tccgcgcacc tgcaacggca acaccttcat cgacgacgag gtgctcg
//
LOCUS       DASRJA010000108         9814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_480266,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000108 DASRJA010000000
VERSION     DASRJA010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480266"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFC01_05055"
     CDS             <1..706
                     /locus_tag="VFC01_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN79048.1"
                     /translation="PDVLNAFNEELYNATAVALRDAATDPDVAVVLLTGAGRAFSAGN
                     DLNEMQRRITDPTFNAQGSHFSTMIDALTELPKPLICAVNGVGVGIGATILGYADLVF
                     MSSTARLKTPFTSLGVAPEAASSYLLPQLIGRQNAAWLLMSSEWVDAAEAQRMGLAWK
                     VCEPDDLLAEARRHAEILASRSISSLVAVKQTIVEPTRPEIAAATAREIAYFAELLGA
                     AANADALAEFTQKRTN"
     gene            712..1764
                     /locus_tag="VFC01_05060"
     CDS             712..1764
                     /locus_tag="VFC01_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZN79049.1"
                     /translation="MGSRPAADEIAELLEPIVHQHFPEAAGARIANWCAAERGLSTET
                     FLFDLERDGDGGPITVKQLVFRRPPAVSLYPDYDLTRQVMVMNRLRDTPIAVPVVCWL
                     DRDDKALGTPYYVMEQLPTIGTASDYPSYHSHGLYFDATPEQRATMWWGCVQAISDVH
                     ALDWHDLRLEKLLMPQRGAHPLEQVVAYCAEMLSWAPPEDRRQELVDAVDWLRDNIYQ
                     PEHLVLCWGDSRLSNVLYGPQFEVTAVLDWEIAYIGDHEADLAWLLFVDWACTEYQGV
                     PRLDGTPSREETVERYEQLSGREMRNLRYNEVLAAVEMGLPVSRLDARLRAEGLLTGD
                     LDLIGFCAERIRQLLD"
     gene            complement(1761..1955)
                     /locus_tag="VFC01_05065"
     CDS             complement(1761..1955)
                     /locus_tag="VFC01_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN79050.1"
                     /translation="MFVCLCNGVTSHVVTEVVANGARTSKQIAEACGAGGDCGRCRRT
                     LRAMIEAHFAVNGSASKVSH"
     gene            complement(1961..2593)
                     /locus_tag="VFC01_05070"
     CDS             complement(1961..2593)
                     /locus_tag="VFC01_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase"
                     /protein_id="HZN79051.1"
                     /translation="MSEYDLHSLILACDFRVDDVDRMWRWLKKHHDPLASIGAHHVVL
                     YTSIWEPGRVLVTIGIRQAGSIREVLRSPAIFEWFEISGADDIPPIFGGEVVEKIDLY
                     GPSPEEHVGRVIVGVMAAVDDVPSLMDKVHDGLERFKRGGVRKIWVYQALDDGREVMI
                     LQEIENELSARQWIDHPDAAAEWMSKAGFGAYPTQFVGRFEHLMSIGERV"
     gene            2832..3317
                     /gene="bfr"
                     /locus_tag="VFC01_05075"
     CDS             2832..3317
                     /gene="bfr"
                     /locus_tag="VFC01_05075"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HZN79052.1"
                     /translation="MQGDPDVLKLLNEQLTSELTAINQYFLHSKMQANWGFTEIAEHT
                     RKESFEEMQHAEAITDRILILDGLPNYQRLFSLRVGQTLREQFEADLAIEYEVMARLK
                     PGIIMCREKEDATTANLFEKILADEEDHIDYLETQLQLMDKLGEQLYLAQCVSRPPSS
                     L"
     gene            3396..5450
                     /locus_tag="VFC01_05080"
     CDS             3396..5450
                     /locus_tag="VFC01_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HZN79053.1"
                     /translation="MTSTAAAGPAADPESGSALISPQRRNLIFVAVLLGMLLAALDQT
                     IVATALPTVVADLGGAGHQSWVVTSYLLASTIVTAVVGKLGDLFGRKMVFALAILFFL
                     AGSVLCGLAGSMTMLVASRALQGIGGGAIMVTATALIGEVIPLRDRGRYQGALGAVFG
                     VTTVVGPLLGGFFTDHLSWRWAFWINVPVAVIVFLVALVAIPALGRTTRPVIDYAGIV
                     LVGMGASGLTLATSWGGTEYAWNSAVIIGLFVASAAALAAFVWVESRATEPILPIRLF
                     RSPVFTVCCVLSFIVGFAMLGALTFLPTFMQFVDGVSATVSGLRTLPMVAGLLLTSMG
                     SGVLVSRTGRYKIFPLVGTAVMFVGFVLLSRMDAATPMLLQSLYLFILGTGIGMCMQV
                     LILIVQNTANFADLGVATSGVTFFRTIGSSFGAAIFGSLFTNFLQDRIGPALAESGAP
                     PAAAQSPQVLHQLPHDVAAPIVNAYADSLGMVFLCAAPAALVGFIVALLLKEVPLREM
                     DAVNAVDLGEGFGMPSTDSADKVLEIAIGRMMRQSPEIRLRNICERPGCNLDVARLWA
                     LIQIYRNNQVFGSARLTEIADRLRVPCEVLEPTFDRLVDTGYALRTGDLLWLTQSGAK
                     QVDAVSSALINRIVEKLASSPTFEGRPDRIQVEAALERIAHRVLVQRDWDAERAELAA
                     AS"
     gene            5517..5999
                     /locus_tag="VFC01_05085"
     CDS             5517..5999
                     /locus_tag="VFC01_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN79054.1"
                     /translation="MSRIGAFADDDAAGWVVKSPDIGVAMAGFTNAVYNKNRLPMRVR
                     ELARMVIALDNECVVCQNTRDSEGIAAGVDEDLYDHAAEWRTWPGYTEQERVAAEFAQ
                     RFASDHTGLRDDEDFWDRAKEHFSPELLTDLALSCAMWLGMGRMLRTLDIGQTCKITL
                     "
     gene            6009..7379
                     /locus_tag="VFC01_05090"
     CDS             6009..7379
                     /locus_tag="VFC01_05090"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZN79055.1"
                     /translation="MAAVTSSAAKPLAAAAIAQLESDGVATLIGTVVNPAGLTHAKTV
                     PLRRMGAFADPGLGASPVWHVFTIDQAGIVFGDATGVVGDQRIRIDLSALRILGDGLA
                     WAPGAFFNQDGSPDPYCSRGVLRRVEERLSAAGIDAVVGHEMEFLLVGPDGSRLPSHL
                     WAQYGLAGVLEFEGFLRDVMNAAAGSGVAIEQFHPEYGVNQFEISLSPQPPVAAADQL
                     VLMRIIIARVARQYGLRVSLSPVPFAGSVGSGSHQHFSMTHGETPLFSDGTGVGGMTA
                     DGESAIAGLLAGLPQAQGVLCGSIVSGLRMQPGHWSGAYVCWGTENREASVRYLIGGP
                     SNPQGANVEVKVIDPSANPYFATATILGLALDGIERKLPLPPETTVDPATLTDDQRTQ
                     AGITLLASRQPDALNALDQSALVRGILGDEAVDAVLAVRRYEHENFGTLNPDELTEKF
                     RLAWSV"
     gene            7516..8244
                     /locus_tag="VFC01_05095"
                     /pseudo
     CDS             7516..8244
                     /locus_tag="VFC01_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            8375..9634
                     /locus_tag="VFC01_05100"
     CDS             8375..9634
                     /locus_tag="VFC01_05100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HZN79056.1"
                     /translation="MSLTAPPPTAVVSPAERLEVLFDELAELVGQRNAIDGRIVEIVA
                     EVDRDELWGATGARSVAALVAWKAGSSSGNAHTIATVARRLEEFPRCAQGMREGRLSL
                     DQVGVIAARAGEGSDEHYAELARSATVNQLRTAVKLEPRPEPESQPDQPEPQRSITKT
                     SDDKGTCWRIKLPHQEAAKFDAALASHRDALIAEWKLDREDRGRTSDQAPPLPGTVEA
                     FMRLVEAGWDAEVARRPHGHHTTVVLHVDVEQRAAALHLGPLLTEAERRYLTCDATCE
                     VWFERDGEVIGAGRATRLINRRLRRALEHRHRTCAVPGCGATRGLHAHHIRHWEDGGP
                     TELFNLVLLCPYHHRLHHRGVITITGPADHLTVTDSSGRRLSAGSLARPPNLPPPDVP
                     PCPGPTGERADWWWYEPFQPQPPPTNN"
BASE COUNT         1709 a         3168 c         3176 g         1761 t
ORIGIN      
        1 gcccgacgtc ctcaacgcgt tcaacgagga gctgtacaac gcgactgcgg tcgcgctgcg
       61 tgatgcggcg accgatcctg acgtggcggt cgtgctgctg accggtgccg ggcgggcgtt
      121 ttccgcgggc aacgacctca acgaaatgca gaggcgcatc accgatccga cgttcaatgc
      181 acagggcagt cacttctcca cgatgatcga tgcgctcacc gagctgccca agccactgat
      241 ctgcgcggtc aacggtgttg gagtcggcat cggcgcaacc attctcgggt acgcggacct
      301 ggtgttcatg tcgtcgacgg cacggctgaa aaccccgttc accagcctgg gcgtcgcgcc
      361 cgaggccgcg tcgtcgtacc tgttgccgca gttgatcggt cggcagaacg cggcgtggct
      421 gttgatgtcg tcggagtggg tcgacgccgc ggaggcgcag cggatggggc tggcctggaa
      481 ggtgtgcgag cccgacgatc tgctggccga agcccgtcgt cacgccgaaa tccttgcgtc
      541 acggtccatc tctagcctgg tcgcagtcaa gcagacgatc gtcgaaccca cccgcccgga
      601 aatagctgcg gcgaccgcca gagagatcgc gtacttcgcc gagctgctcg gtgcggcggc
      661 caacgccgac gcgcttgccg aattcacaca gaaacgaacg aattaactga catgggctcg
      721 cgtcctgcgg cggacgaaat cgctgagcta ctcgaaccga ttgtgcacca gcactttccg
      781 gaggccgccg gcgcccgaat agctaattgg tgcgctgccg aacgcggact gtccaccgag
      841 acctttctgt tcgacctaga gcgagatggc gacggcgggc caatcacagt gaagcagttg
      901 gtctttcgcc ggccgcctgc cgtcagcctg tacccggact acgacctgac acgccaggtg
      961 atggtgatga accggctgcg ggacacaccg atcgcggtgc cggtcgtgtg ctggctggac
     1021 cgcgacgaca aggcccttgg cacgccgtac tacgtgatgg aacagctgcc gacgatcggc
     1081 accgcgagcg attacccgtc gtatcactcg cacggcctgt atttcgacgc cacgccggag
     1141 cagcgggcca ccatgtggtg gggctgcgta caggccatct ccgatgtgca cgcgctggac
     1201 tggcacgacc tgcgcttgga aaagctgctg atgccgcagc gtggcgcgca tccgctggaa
     1261 caggtcgtcg cctactgcgc cgagatgctg tcgtgggcgc cgccggaaga ccgccgccag
     1321 gaactcgtcg acgccgtgga ctggttgcgc gacaacattt atcagcccga gcacctcgtg
     1381 ctgtgctggg gcgacagcag gctttccaac gtcctgtatg ggccgcagtt cgaagtcacc
     1441 gccgtgctgg actgggagat cgcctacatc ggtgaccacg aggccgattt ggcgtggctg
     1501 ctgtttgtcg actgggcgtg cacggagtac cagggcgtgc cccgactgga cgggactccg
     1561 agccgggagg aaaccgtcga gcggtacgag cagttgtcgg gccgcgaaat gcgaaatctg
     1621 cgctacaacg aagtgctcgc agcggttgag atgggactgc cggtctcccg gttggacgcc
     1681 aggctgcgcg ccgaaggact gctcaccggt gatctggacc tgatcgggtt ctgcgccgaa
     1741 cgcatccgcc agctgctgga ctaatggctc accttcgacg cagagccgtt gaccgcgaaa
     1801 tgggcctcga tcatcgcccg cagtgtgcga cggcaccgcc cgcagtcgcc accggcgccg
     1861 caagcctcgg cgatctgctt tgaggtcctc gcgccgttgg ccaccacctc ggtgacgacg
     1921 tggctggtga ctccgttgca caggcagacg aacatcgtgg tcagacccgc tcgccgatgc
     1981 tcatgagatg ctcgaacctg ccgacgaatt gagtgggata ggcgccgaag ccggccttgg
     2041 acatccactc cgcggcggcg tccgggtgat cgatccactg gcgggcgctg agttcgttct
     2101 cgatctcctg caggatcatc acttcccgtc cgtcgtcgag tgcctgatac acccagatct
     2161 ttcgcacgcc accgcgcttg aaccgctcga ggccgtcgtg caccttgtcc atcagggacg
     2221 gcacgtcgtc gaccgccgcc atcacgccga cgatcacccg gccgacgtgc tcctcgggcg
     2281 agggcccgta gaggtcgatc ttctcgacta cctcgccgcc gaatatcggt ggaatatcgt
     2341 cggcgccgga aatctcgaac cattcgaata ttgcgggcga ccgcaaaacc tcacgaattg
     2401 acccggcctg gcggatacca attgtcacta atacccggcc gggttcccaa atggacgtgt
     2461 acagcaccac gtgatgggca ccgattgagg ccagcgggtc atggtgcttc ttgagccatc
     2521 gccacatccg gtcgacatcg tcgacacgaa aatcacaggc aaggatgagt gagtgtaaat
     2581 cgtactcact catggttttc ctgtctgccg gacggggtag cgcaatgtta gtgcagtcta
     2641 accttactaa gcgtagccag tccaggctgc tggcctgatc tgcggcccct cgcccgccgg
     2701 tcgttgccta aatcaccgaa aacacgcctg gcgcaacccc attacctgcg ccctcgtcgg
     2761 actcctgcac tagattgagt gcgcacggaa gacgcggtac ttgatcagcc ctcaaggtgc
     2821 ttaggagcga catgcaaggc gaccccgacg ttctgaagct gctgaacgag cagttgacaa
     2881 gcgaactcac ggccatcaac cagtacttcc tgcattccaa gatgcaggcg aattggggat
     2941 tcaccgagat cgccgagcat acgcgcaaag aatctttcga agaaatgcag catgcggagg
     3001 caatcaccga ccgcatcctc attttggacg ggctgccaaa ttatcagcgc cttttctcgt
     3061 tgcgggtcgg ccagactctt cgcgaacaat ttgaagccga cctggccatc gaatacgagg
     3121 taatggcgcg gctgaagccg ggaatcatca tgtgccgcga aaaagaagac gccaccaccg
     3181 cgaacctgtt cgagaagatc ctcgccgacg aagaggatca catcgactac ctcgagaccc
     3241 agctgcagct gatggacaag ctcggcgagc agctctacct cgcgcagtgc gtgtcgcggc
     3301 ccccgtcgtc gctgtaggcc ggttgccaac gacgcccgaa cggcaactct cacctcggtt
     3361 gtaactttcc tagcttgtct aacgataagg caggcatgac gagtaccgca gcagcgggcc
     3421 ctgcggcgga tcccgaatcc ggcagtgccc tgatcagtcc tcagcgccgc aacttgatct
     3481 ttgttgcggt gctgctgggc atgctgctgg ccgcgctgga ccagacgatc gtcgccaccg
     3541 cgctgccgac ggtggtcgcg gacctaggcg gcgccggcca ccagtcctgg gtggtcacca
     3601 gctatctgct ggcgtcgacg atcgtcaccg cggtcgtggg caagctcggc gacttgttcg
     3661 gccgcaagat ggtcttcgcg ttggcgatcc tgttcttctt ggccggttct gtgctgtgcg
     3721 ggttggccgg ttcgatgacc atgctggtgg cgtcgcgggc actgcagggc atcggcggcg
     3781 gcgccatcat ggtgacggca acggcgctga tcggtgaggt catcccgctg cgcgaccggg
     3841 gccggtatca aggagcactg ggtgccgtgt tcggcgtcac cacagtggtc gggccgctgc
     3901 tcggcggctt cttcaccgac catctcagtt ggcgttgggc gttttggatc aacgtgccgg
     3961 tggccgtgat cgtcttcctc gtcgcgctcg tggcaatacc ggcgctgggc cgcaccacgc
     4021 gcccggtcat cgactacgcc ggcatcgtcc ttgtcggcat gggcgcatcg ggactcacgt
     4081 tggccaccag ttggggtggc accgagtacg cctggaactc agcggtgatc atcgggctgt
     4141 tcgtcgcgtc cgcggcggcg ctggcagcct tcgtatgggt ggaaagccgt gcgacggaac
     4201 cgatcctgcc gatccggctg ttccgcagcc cggtgttcac ggtgtgctgt gtgctgtcgt
     4261 tcatcgtcgg gttcgcgatg ttgggcgcgt tgacattcct gcccaccttc atgcagttcg
     4321 tcgacggcgt gtcggcgacg gtgtcagggc tgcgcacgct gccgatggtc gccggcctgc
     4381 tgctgacgtc gatgggcagc ggcgtgctcg tgagccgcac cggccgctac aagatcttcc
     4441 cgctcgtagg gacggcggtg atgttcgtcg ggttcgtgct gttgtcgcgg atggacgccg
     4501 ccacaccgat gctgttgcaa tcgctgtatc tgttcatcct cggcaccggg atcggaatgt
     4561 gcatgcaggt gctcattctg atcgtgcaga acaccgcgaa cttcgccgat ctcggagtcg
     4621 cgacctccgg cgtcacattc ttccggacca tcggaagttc cttcggcgca gcgattttcg
     4681 ggtcgctgtt caccaacttc ctccaggacc gcatcggtcc ggcgctggca gaaagcgggg
     4741 cgccaccagc ggccgcgcag tcaccgcagg tgctgcacca gttaccgcac gacgtcgccg
     4801 ccccaatcgt caacgcgtac gcggattccc tgggcatggt tttcctgtgc gcagccccgg
     4861 cggctttggt cgggttcatc gtcgcgctct tactcaaaga ggttccgctg cgggagatgg
     4921 acgccgtcaa cgccgtcgat ctgggtgagg gcttcggcat gcccagcacc gactcggcag
     4981 acaaggtgct ggagatcgcg atcggccgga tgatgcggca gtcaccggag atccggctgc
     5041 gcaacatctg cgaacgaccc ggctgcaacc ttgatgttgc acgtctgtgg gcgctgatcc
     5101 agatctaccg aaacaatcag gtgttcgggt cggcgcgact gaccgaaatc gccgatcggc
     5161 tgcgggtgcc atgcgaggtg ctcgagccga cgttcgacag actcgtcgac accggctacg
     5221 cgctgcgcac cggcgatcta ctgtggctga cccagtcggg tgcgaagcag gtcgacgcgg
     5281 tgtcgtcggc gttgatcaac cggatcgtcg agaagctggc gtcatctccg acgttcgagg
     5341 ggcgcccgga ccgcatccaa gtggaggcgg cgctggaacg catcgcgcac cgcgtgctgg
     5401 tacagcggga ctgggacgcc gagcgggccg aactggccgc tgccagttga ccagggcatc
     5461 taacccggtt tctagaacgt gttccaattc gtttccaccg ggcgtacgat gccgccatga
     5521 gccgaattgg agccttcgct gacgacgacg ccgcaggctg ggtggtgaag tctccggaca
     5581 tcggagtcgc catggccggg ttcaccaacg cggtctacaa caagaaccgc ctgccgatgc
     5641 gggtccgcga gttggcccgg atggtcatcg cgctcgacaa cgaatgcgtc gtgtgccaga
     5701 acacccgcga ctcggagggc atcgcggcgg gcgtcgacga ggacctctat gaccacgccg
     5761 ccgaatggcg gacgtggccg ggctacaccg agcaagaacg cgtcgccgcc gagttcgcgc
     5821 aacgcttcgc cagtgaccac accgggctac gggacgacga ggacttctgg gaccgcgcca
     5881 aagaacactt cagccccgaa ctgttgaccg atctggcgct gtcgtgtgcg atgtggcttg
     5941 gcatgggccg gatgctgcgc acgctggaca tcggccaaac ctgcaagatc acgctctagc
     6001 gttggacaat ggctgctgtg acatcgtccg cagccaagcc cctggccgca gcggccatcg
     6061 cccaactgga gtccgacggg gtcgccacgc tcatcggcac ggtcgtcaat ccagccgggc
     6121 tcacgcatgc caagacggtt ccgctccgcc ggatgggcgc attcgccgac ccgggactcg
     6181 gcgcgagccc tgtctggcac gtcttcacca tcgaccaggc gggcatcgtc ttcggcgacg
     6241 ccaccggtgt ggtgggggac cagcggatcc gcatcgacct gagcgcactg cgcatcctcg
     6301 gcgacggatt ggcctgggcc cctggtgctt tcttcaatca ggacggctcg cctgacccgt
     6361 attgcagccg cggcgtgctg cgccgggtcg aggagcgatt gtccgccgcc ggcatcgacg
     6421 ccgtcgtcgg tcacgagatg gagttcctgc tcgtcgggcc ggacggcagc cgattgccat
     6481 cccacctgtg ggcgcagtac ggcctggccg gggtgctcga attcgagggc ttcctgcgcg
     6541 acgtgatgaa cgcggcagcc ggttcgggtg tggccatcga acagttccac cccgagtacg
     6601 gcgtgaacca attcgagatc tccctgtcac cacagccacc cgtcgcggct gccgaccagc
     6661 tggtgttgat gcgcatcatc atcgccaggg tggcccggca gtacgggttg cgagtcagcc
     6721 tgtcaccggt gccgttcgcg ggcagcgtcg gatcgggttc gcatcaacac ttttcgatga
     6781 cgcacggcga aaccccgctg ttctcggacg gaaccggcgt cggcggcatg acggccgatg
     6841 gtgaatcggc cattgcggga cttctggccg gtctgccgca agcgcagggc gtgttgtgcg
     6901 gctccatcgt gtcgggattg cggatgcagc cgggtcattg gtcgggcgct tacgtctgct
     6961 ggggaaccga gaaccgcgaa gcctcggtgc ggtacctcat tggcggacca agcaatccac
     7021 aaggggccaa tgtcgaggtg aaagtcatcg acccgtcggc caatccgtac ttcgcgaccg
     7081 ccacgatcct cgggctcgcc ctcgacggca tcgaacgcaa actgccgctg ccgcccgaaa
     7141 cgaccgtcga ccccgccacg ctcaccgatg accaacgcac gcaagccggc atcacgctcc
     7201 ttgcgtccag acagccggac gccctcaatg cgctggacca atccgccttg gtgcgcggca
     7261 ttctcggcga cgaggccgtc gacgccgtgc tggcagtgcg acgctacgag catgagaact
     7321 tcggcactct caacccggac gaactcaccg agaagttccg gttggcctgg agtgtctgag
     7381 gttgagcacg ctggcggagc acatcgacaa cgtgccgctc gtcgatcatc atgtgcacgg
     7441 ctgctgactg gacgcgcctg accggggacg gttgcggatc tcgacgagtt gaccgctctc
     7501 tcggtgggcc gcatccacga gatcgtccgt ctggagtcgg tcgccgaaga cgccattcgg
     7561 atgcctgacg actatgcgac ggcgttcgcc cgcctcctcg acgagcgcgc gaagaccgcg
     7621 atcgcgacaa agtccatact ggcctaccgc ggcggattcg acggcgatct atccgagccg
     7681 atacccgcac aggtagcaga tgcggcgcag cgatggcggg acagcggcaa tccccggctg
     7741 accgaccggg tgctgctgcg attcggcctg tatcaggctc tgcgccttgg caagccgctg
     7801 cagtttcaca tcgggttcgg cgaccgcgac tgcgatctgt acagaacgaa cccgctgtac
     7861 ctgctggact ttctgcgcaa cagcggcgac accccgatca tgttgctgca ctgctatccc
     7921 tacgagcgcg aggccggcta tctggcgcag gcgttcaaca acgtgtacct agacggcggg
     7981 ctggtgatca actatctcgg cgcgcggtcg gcgtcgttca tcggccggct gctggagatg
     8041 gcgccgttcc gcaagatcct gtactcatcg gacgggttcg gtccggccga gctgcactgc
     8101 ctcggggcgc ggctgtggcg caacggaatc cgcgatgtcc tacaaggctt catcgacgcc
     8161 gacgaatgga gtgaacgcga cgcgatccgc gtggtcgacc tgatcgcaca cgagaccgcg
     8221 cgtcgtgtct attgcatgga ctgaggtgct gaaggctgcg gtgggcagcg tttggcggcc
     8281 aatcaggctg tccctcttgg cagatgacga ctgccacgcc ccggcgcgga ttggacagcc
     8341 gaaacttgtc gtacccccct gcaatgatga agttatgtcc ttgaccgcac cgccgcccac
     8401 ggctgtggtg agccctgccg agcgtcttga ggtgttgttt gacgagttgg cggagttggt
     8461 cggtcagcgc aacgcgattg atgggcgcat cgtggagatc gtcgcagaag tggatcgcga
     8521 cgagctgtgg ggcgccacgg gggcgcggtc ggtggcggcg ttggtggcct ggaaggcggg
     8581 ttcgtcatcg ggaaacgctc acacgatcgc caccgtggcg cgccggttgg aggagtttcc
     8641 ccgctgtgcc caaggcatgc gggaaggccg attgtcgctg gatcaggtcg gggtgatcgc
     8701 ggcgcgggcg ggcgagggat ccgatgagca ctacgcggag ctggcgcgca gtgccacggt
     8761 caaccagctg cgcaccgcgg tcaagctcga accgcgaccc gaacccgagt ctcagccgga
     8821 ccagccagaa ccgcagcggt cgatcacgaa gacctccgac gacaagggca cctgttggcg
     8881 gatcaaactt ccgcaccagg aggcggcgaa gttcgacgcc gcactggcgt ctcatcgtga
     8941 tgcgctgatc gccgagtgga aactcgatcg cgaggaccgc gggcggacat cagatcaggc
     9001 gcctccgttg ccgggcactg tcgaggcgtt catgcgactg gtggaggccg ggtgggacgc
     9061 cgaggtggcc cgccggccgc acgggcatca caccacggtg gtgctgcacg ttgacgtcga
     9121 gcagcgcgcg gcggcgctgc atctgggtcc gctgctcacc gaggccgaac gccgatacct
     9181 gacctgtgat gccacctgtg aagtgtggtt cgaacgcgac ggcgaggtca tcggcgccgg
     9241 ccgagcgacc cggctgatca accggcggct tcgccgcgcg ctggagcacc gccatcggac
     9301 gtgtgcggtt ccgggttgtg gtgccacccg cggtttgcac gcccaccaca tccggcactg
     9361 ggaggacggc ggtcccaccg agttgttcaa cctcgtgctg ctgtgcccgt atcatcaccg
     9421 gttgcaccat cgaggcgtca tcaccatcac cggacccgcc gaccatctca ccgtcaccga
     9481 cagctccggc cgacgactca gcgcgggatc gctcgcgcgt ccgcccaacc ttcccccacc
     9541 cgatgtccca ccgtgccccg gacccaccgg tgagcgcgcc gattggtggt ggtatgaacc
     9601 cttccagccg cagccaccac caaccaacaa ctaggttgct taccagggcc ggttattacc
     9661 agcaccgtag cgaagctcaa acgggttggg gcatagagaa tttcgttcgc gatacgcgca
     9721 gctaccgcat cgtgaagatt cgaagctgac aaccagaccc gcaccgccgc cgacccgcta
     9781 accgacgacg tccgcaacgc cctcatcacg accg
//
LOCUS       DASRJA010000109         3754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4189036,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000109 DASRJA010000000
VERSION     DASRJA010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4189036"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFC01_05105"
     CDS             <1..500
                     /locus_tag="VFC01_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN79057.1"
                     /translation="LMPFLTELFEATRQTVHLAMLNGTDVVYLAKLYGHRNAVAPSRI
                     GGRLPAHCTAVGKALLAYDPAAVEKITSGRLTALTAHSITDGNELARALERVRREGVA
                     TEREESRPGLGCVAAPVFGRNGLPIAALSVSVPLRADLRPLTHSLRRVCASASRTMSH
                     SHRNG"
     gene            complement(501..944)
                     /locus_tag="VFC01_05110"
     CDS             complement(501..944)
                     /locus_tag="VFC01_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79058.1"
                     /translation="MKRLSLDLGPGWTVTPGEKDVLGIIRRDDDGECVEGVARYASGG
                     PLRDAYPVGAHDVEIRISRRPPPSSRLTAFLDELGVAIVAADPACRRVVVAVAPDDLA
                     AISAAESVGFRHVVDVDVPEELSLLVREPEWVTQVDADLDRVPGS"
     gene            complement(941..1774)
                     /locus_tag="VFC01_05115"
     CDS             complement(941..1774)
                     /locus_tag="VFC01_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016894992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN79059.1"
                     /translation="MTGWLQGYVALITGGSTGIGRAVAERFIAEGSSVAILGRDPDAL
                     HAAIEASADPAKLHAISADVRDSADLHRAVGETVDRFGKLDTLVANAGIWDYHRQLTR
                     MTGAQLSATFDELFAVNVKGYLLAAEAAWRELVKTRGSIVMTLSNASFYVNGGGPIYT
                     ASKHACLGLMREFAYELAPKVRVNGVACGAMNTALRGPAALDMQDRTLAASFAKKKAG
                     DAPPPIPLYDSSTDPRDFTGSYVLLASREQSGPITGQAISVDGGIGVRGFASAAGGDH
                     L"
     gene            complement(1771..2622)
                     /locus_tag="VFC01_05120"
     CDS             complement(1771..2622)
                     /locus_tag="VFC01_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN79060.1"
                     /translation="MPTLLTRTVTTSYGDIAMSETGEGPVLVMLHGGGLGASGVSNYH
                     QNLPALAKTFRVVLPDQPGFGGSYRPTEADLDAQSITQISVGALFEALDAIGIDRFHL
                     LGNSLGGAAAIAMAQAQPDRITGLVLMAPGGGWLPFGPTPTEGQKEMFRYYNGDGPSE
                     KKMSNFIRTMVFDHKQFGADVVKARYEASLDPSHIEFYHRYNAAFGKRHGMDPLWRDL
                     HKVTAPTLLLWGRDDRTITLDGSQIMLKQIRNVQLHVFGNCGHWVQLERRRDFELLVA
                     QFLTELR"
     gene            complement(2702..3271)
                     /locus_tag="VFC01_05125"
     CDS             complement(2702..3271)
                     /locus_tag="VFC01_05125"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HZN79061.1"
                     /translation="MTSAATILASIDGARRASHDIHFEVEQFYYTEAELLDDGRFTDW
                     LDLLDDDLDYWMPTRTNRLRRQQALSISARGEAAFYDETKESLAWRIRRFDSGMAWAE
                     DPPSRTRHLVTNVVVHHVDSDQGSGSDLAARSAFLVYRNRLEHEVNLFAGCRTDILRR
                     SPDGGLKVARRVILLDQSVLLAKNISTFF"
     gene            complement(3294..>3754)
                     /locus_tag="VFC01_05130"
     CDS             complement(3294..>3754)
                     /locus_tag="VFC01_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016894995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HZN79062.1"
                     /translation="VDGGIVVDPMQVTSNPAVLAVGDVARTCAGGVLLHRSEHWEAAQ
                     HDAQRAAATILGAPPPAASASWFWTDRYGTHVEVVGNLAGAEEFASRGRLGEQPYACF
                     GLRGGRVAGAVAVDDSASVRAARRMIDRGITVSAAELGDRSVDLRRLIKR"
BASE COUNT          655 a         1254 c         1191 g          654 t
ORIGIN      
        1 agctgatgcc cttccttacc gaactgttcg aagcgactcg ccagaccgtg cacctcgcga
       61 tgctgaacgg taccgatgtc gtctacctgg ccaaactgta cggacatcgc aacgcggttg
      121 cgccgtcacg catcggcggg cgcttacccg cgcattgcac cgccgtcggc aaggccttgt
      181 tggcctatga ccccgctgcg gtggaaaaga tcaccagcgg ccgactcacc gcgctcaccg
      241 ctcactcgat cactgatggc aacgagttgg cgcgtgcgct cgagcgcgtg cgacgcgagg
      301 gcgtggcgac cgagcgcgag gaaagccgtc ccgggttagg ctgcgttgcg gcaccggtgt
      361 tcggccgtaa cgggttaccg atcgccgcgt tgtcggtgtc agtccccctg cgcgccgacc
      421 ttcgtccgct cacgcacagt ttgcggcgtg tctgcgcttc cgcctcccgg accatgagcc
      481 actctcaccg caatgggtga tcagctgcct ggcactcgat cgaggtcggc gtccacctga
      541 gtgacccact cgggttctcg gacaagcaaa ctcaattcct cgggaacgtc gacatcgacg
      601 acgtggcgaa agcccaccga ctccgccgcc gagatcgcgg ccaggtcatc gggtgcaaca
      661 gcaacgacaa cacgtcggca ggcggggtct gcggccacaa tcgcaacgcc aagttcgtcg
      721 aggaaagcag tcagccgaga cgagggcggg ggtcggcgtg agatgcgaat ctcaacatca
      781 tgggcaccca ccgggtaggc gtcgcgcaga ggaccgccgc tggcataccg ggctaccccc
      841 tcgacgcact cgccgtcgtc atcacgacgg ataatgccga ggacgtcttt ctccccgggt
      901 gtgaccgtcc accccggccc caagtccagc gacagccgct tcacagatgg tcacctccag
      961 cagccgaagc gaacccgcgc acgccgatac cgccgtcgac cgaaatcgcc tgtccggtaa
     1021 tgggtccgct ctgctcgcgg ctggccagca gaacgtagga tccggtgaaa tctcgcgggt
     1081 cggtgctcga gtcgtacagc gggatcggtg gcggcgcatc acccgctttc ttcttcgcaa
     1141 aggacgccgc cagtgtgcgg tcctgcatgt ccagggccgc cggcccgcgc aacgccgtat
     1201 tcatcgcccc acaggccacg ccgttgacgc ggaccttcgg cgccaattcg tatgcgaact
     1261 cacgcatcag ccccaggcac gcgtgcttgc tggccgtgta gatcgggccg ccgccgttga
     1321 cgtaaaacga ggcgttggac aatgtcatca cgatgctgcc gcgggtcttg accagctccc
     1381 gccacgctgc ctcagcggcc agcagataac ccttgacgtt gacggcgaac agctcgtcga
     1441 aggtagcact gagctgcgcg ccggtcatcc gggtcagctg ccggtggtaa tcccagatcc
     1501 cggcattggc cactagcgtg tcgagcttcc cgaagcggtc aacggtctcg ccgaccgcgc
     1561 gatgcaggtc cgccgaatcg cgcacatccg cgctgatcgc gtgcagcttg gccggatcgg
     1621 cgctggcctc gatggcggcg tgcagcgcat cggggtcgcg gccgaggatg gcgaccgagg
     1681 aaccctcggc gatgaatcgt tcggcgacgg cccgcccgat cccggtactc ccgccggtaa
     1741 tcagcgcgac gtatccttgc agccagccgg tcaacgcaac tccgtcagga actgcgccac
     1801 cagcagttca aaatcgcgcc ggcgctctaa ctgcacccag tgcccgcagt tgccgaatac
     1861 gtgcagctgc acgttgcgga tctgtttgag catgatttga gatccgtcga gggtgatggt
     1921 gcggtcatca cggccccata acagcagggt gggggcggtg accttgtgca gatcacgcca
     1981 cagcggatcc ataccgtgtc gcttaccgaa ggcggcgttg tatcggtgat agaactcgat
     2041 gtgactgggg tccagcgatg cctcgtaccg cgctttgacg acgtcggcgc cgaactgctt
     2101 gtggtcgaag accatggtgc ggatgaagtt cgacatcttc ttctcgctgg ggccatcacc
     2161 gttgtagtac cggaacatct ccttttgccc ctcggtcgga gtcgggccga atggcagcca
     2221 gccacccccg ggcgccatca gcaccagtcc ggtgatccga tccggctgcg cctgtgccat
     2281 cgcgatggcc gccgcgccgc caaggctgtt gcccagcagg tgaaaccggt cgataccgat
     2341 ggcgtccagc gcctcgaaaa gcgcgccgac cgagatctgg gtgatcgact gcgcgtcgag
     2401 gtcggcctcg gtggggcggt agctgccgcc gaacccgggc tgatcgggca gcacgacccg
     2461 aaacgtcttg gccagcgcag gcaggttctg gtggtagttg ctgaccccgg aggctcccag
     2521 gccgccgccg tgcaacatga ccaggacggg tccctcgccg gtctctgaca tggcgatgtc
     2581 gccgtaggag gtggttaccg tccgcgtgag caacgtgggc atttcgtcga tctcagtgtc
     2641 gaattgcgtg ctggccaccg gcacgtcctt tcactcgtcg gtctgaagga aatctgtcga
     2701 ctcagaagaa ggtggagatg ttcttggcca gcaggacgct ctggtcgagc aagatgaccc
     2761 tgcgggcgac cttcaacccg ccatcggggc tgcgacgcag gatgtcggtg cggcaccccg
     2821 cgaaaaggtt cacctcgtgt tcgaggcggt tgcgatacac caggaacgcc gagcgggcgg
     2881 cgagatcaga gccactcccc tgatccgaat cgacatggtg caccacgacg ttggtgacta
     2941 gatgccgggt ccgcgagggc gggtcctcgg cccaggccat gccggaatcg aatcgccgga
     3001 tccgccacgc cagactctcc ttggtctcgt cgtagaacgc ggcctcgccc cgcgccgaga
     3061 tcgacagcgc ctgttggcga cgcaaccgat tggttctggt gggcatccag tagtcgaggt
     3121 cgtcgtccaa cagatcgagc cagtcagtga agcgtccgtc gtcgagcaac tcagcctcgg
     3181 tgtagtagaa ctgctcgacc tcgaagtgaa tgtcgtggct ggcccgccgt gcaccgtcga
     3241 tgctggccaa tattgttgct gcgctggtca tgtgggtact ctccgtgtcg tcatcatcgt
     3301 ttgatcagcc tgcgtaggtc taccgatcga tcgcctagtt cggccgcgga taccgtgatg
     3361 ccgcggtcga tcatcctgcg cgcggcccta acagacgccg agtcgtcgac cgccaccgct
     3421 ccggcaaccc gcccgccacg caatccgaag cacgcgtagg gctgctcacc gaggcgaccc
     3481 cgagatgcga actcctcagc accagcgagg tttccgacga cctcgacgtg tgtcccgtac
     3541 cggtcggtcc agaaccagga tgcgctggca gctggcggcg gcgcgccgag gatcgtcgcg
     3601 gccgcgcgct gtgcgtcgtg ctgggccgcc tcccagtgct cgctgcggtg cagcaggaca
     3661 ccgccggcac aagtgcgcgc gacatccccg accgcgagga ccgcaggatt cgacgtcacc
     3721 tgcatcgggt cgacaacgat gccgccatct actg
//
LOCUS       DASRJA010000110        21291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3575505,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000110 DASRJA010000000
VERSION     DASRJA010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 21291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21291
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3575505"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFC01_05135"
     CDS             <1..658
                     /locus_tag="VFC01_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HZN79063.1"
                     /translation="SVGAGLDKWIVFVPADSADLVRDAMFAAGAGSVGTVERVPADRV
                     EVTAPTRLRAHVLAAMRAAHPNEEPAFDIYALATVPAGVGLGRVGLLPRAEPLSAFVS
                     RVHDALPGTSWGVRASGDSEAMVSRVAVCGGAGDSLLDTVADAGVQAYVTADLRHHPA
                     DEHRRASDVALVDVAHWASEYPWCTQAADVLRSHFGDALPVRVSAIRTDPWNVERNET
                     "
     gene            655..1392
                     /locus_tag="VFC01_05140"
     CDS             655..1392
                     /locus_tag="VFC01_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HZN79064.1"
                     /translation="MKAEVVQQRSLLELAELDAELSRIEHRAKNLPAQQQLEEAQTAH
                     REANDRLAAVQIALEDLDAQVAKFESEIDAVRQREDRDRSMLSSGTVDAKQLTDLQHE
                     LETLERRQSSLEDSLLEVMERREELQGQQAAKLAEIDELQSKLTEAQRACDEARTEID
                     QSRHQSISRRDELVASLDADLVALYERQRSRGGAGAGLLQGRRCGACRIEIDQGERAR
                     IAAAAEDDVLRCPECGAILLRVKGTGA"
     gene            1389..2477
                     /locus_tag="VFC01_05145"
     CDS             1389..2477
                     /locus_tag="VFC01_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional RNase H/acid phosphatase"
                     /protein_id="HZN79065.1"
                     /translation="MKVIVEADGGSRGNPGPAGYGTVVWTADHGTVLAESKQAIGRAT
                     NNAAEYRGLLAGLEEAVKLGATDVDVMMDSKLVVEQMSGRWKVKHPDIAALHQQATAL
                     SGRFEHISYTWIPRAKNSHADRLANEAMDAAAEMDTAAEAPKESSTPSSAPGWTGARG
                     APTRFLLLRHGQTELSTQRRYSGRGNPALTDVGRRQAEAAAQFLAQKGGIEAVITSPL
                     QRAYDTAAAAAKALGLDVTVDHDLIETDFGAWEGLTFGEAAERDPDQHRRWLRDTSVT
                     PPDGESFDSVAERVRRAQARIINEHTGQNVLVVSHVTPIKTLLRMALDAGPGILYRLH
                     LDLASLSIAEFYPDDVASVRLVNQTAYL"
     gene            complement(2478..3053)
                     /locus_tag="VFC01_05150"
     CDS             complement(2478..3053)
                     /locus_tag="VFC01_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZN79066.1"
                     /translation="MEAKSEDGVDLRVRRRLRELRMQRGLTLEDVASRSSIDVSTLSR
                     LESGKRRLALDHLPRLAAALSVSTDELLRAPEAQDPRVKGGSHTHNDVTYWPLTRHGP
                     AGGLHAYKIRVSARRRNPPAELPVHEGQDWMYVLSGRMRLILGEKDFVIKPGEAVEFS
                     TWTPHWFGAVDGPVEAITLFGVHGERLHLHN"
     gene            3101..3724
                     /locus_tag="VFC01_05155"
     CDS             3101..3724
                     /locus_tag="VFC01_05155"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN79067.1"
                     /translation="MNTTDAKEHWEQHYGERDRRWSGRVNVRLPEVAAELRPGTALDL
                     GCGEGADAWWLAERGWTVVAVDISDTALQRAATEADARGLSDRIEFVQQDLSDGFPSG
                     TFDLISAQFLHSMIPFDRPRVLKRAAGAVRPGGSLLIVDHAGPPPWASKLDHHHEFPS
                     AEEVIGSLELDDFEWERVRVEAVEREVTTPDGQTATWFDNVIVLRRR"
     gene            complement(3721..3975)
                     /locus_tag="VFC01_05160"
     CDS             complement(3721..3975)
                     /locus_tag="VFC01_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HZN79068.1"
                     /translation="MKHYTLIGSNGIPIDSATPGTLGGHKATRIYGRLDCPSALRALA
                     KGGYVTHREFFADEATAIAAGYRPCARCLPERYRAWKART"
     gene            complement(3979..4695)
                     /locus_tag="VFC01_05165"
     CDS             complement(3979..4695)
                     /locus_tag="VFC01_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZN79069.1"
                     /translation="MAQTKWQKRVDSGDWDAITAEVNDFGGALLPRLLTKAEAERIRG
                     LYPDDHLFRATIDMRRYRFGDGEYRYFQQPYPEPIEQLKQAIYPRLLPIARDWWTKLG
                     RKAPWPDSLDDWLDMCHDAGQKKSTAILLKYGEGDWNALHRDLYGDLVFPLQVVINLS
                     EPGVDHTGGEFLLVEQRPRAQSRGTATLLPFGHGFVFTTRERPVESTRGWSASPVRHG
                     VSAVRSGERFTLGLVFHDAA"
     gene            complement(4695..5207)
                     /locus_tag="VFC01_05170"
     CDS             complement(4695..5207)
                     /locus_tag="VFC01_05170"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZN79070.1"
                     /translation="MNTRHAVIESPLGELTLVAEDDALTGLYFRHHWYRPAAESLGER
                     VEAKSDGLLAEAQTQLTDYLAGRRHGFNLPTKLNGDEAQRRVWHLLSTIPYGETITYG
                     ELADAVADGTTAREVGQAVGRNPLSIIVPCHRVVGKNGQLTGYAGGLNRKQLLLELEE
                     PSEVKAARLF"
     gene            5274..5876
                     /locus_tag="VFC01_05175"
     CDS             5274..5876
                     /locus_tag="VFC01_05175"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN79071.1"
                     /translation="MFEVVADADLIDVMSEATRDESTAADASVEQQAVLPLSAQVPPF
                     YRAAALERGKMTDMGEAKGPFGGSSHSSASAEDVWAVWTNPSEWPGDVIAMAKIDGPF
                     AVGAKITTRVKGYPPLTSTVTRIEPPMLWTGVAKAPGLTQTIDHLIEPTDSGTLITER
                     TVFTGPLASIAARLVGGRLRKTFEATTAHCGRLAESRASH"
     gene            5943..6302
                     /locus_tag="VFC01_05180"
                     /pseudo
     CDS             5943..6302
                     /locus_tag="VFC01_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(6281..7093)
                     /locus_tag="VFC01_05185"
     CDS             complement(6281..7093)
                     /locus_tag="VFC01_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZN79072.1"
                     /translation="MYRSAAYWTSTLIVAAECIGGGVMGALQLRPFRDTATQLGYPVY
                     FMSILGVWYISAGLIVLAPGLPRLKEWAYAGLVINYTGAAASHIAVGDDAETLLGPTV
                     FLVLTAASWWLRPPARRQIPPATGTSRPGRFAAYWVTTALVAAELAVGGIWDLLRIDY
                     VRGVIEHLGYPPYLLTIMGVWKLAGAVVLLIPRFPRLKQWAYAGATINYASAVASHVI
                     VDGTPAPLIAPLALLALTVASWALCPPRAAALSKPTAAESNTTSCSQNGGGS"
     gene            complement(7130..7627)
                     /locus_tag="VFC01_05190"
     CDS             complement(7130..7627)
                     /locus_tag="VFC01_05190"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZN79073.1"
                     /translation="MRTSTAAVGSAAFFLVAPGTFVGLGPWLITHWEIPGSSPPLRVV
                     LGVVLIVLGLIPPVHAFVQFAKAGGTPMPVAPTERLVVTGFNRFVRNPMYVGLLTAIL
                     GQALLFDSPWLVLYAVIGWIATASFVHWYEEPTLVRTYGEQYEEYRRNVPAWTPRLTP
                     WKAQG"
     gene            complement(7628..8065)
                     /locus_tag="VFC01_05195"
     CDS             complement(7628..8065)
                     /locus_tag="VFC01_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN79074.1"
                     /translation="MIKIASSHLWVHDQEVALKYWTEKVGMEVRQDVSFPEEMGEFRW
                     LTVGPRGQDDVSIVLMAVPGEPVMDEATRKEVLNLTAKGFAGTVFLTTEDCQASCEEM
                     KARGVEFTEEPHQMPYGIDCGFRDPSGNSVRLTQLTEIPANTG"
     gene            complement(8076..8528)
                     /locus_tag="VFC01_05200"
     CDS             complement(8076..8528)
                     /locus_tag="VFC01_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZN79075.1"
                     /translation="MTQVPPVRYLQRAKDLVDARYAEAITVDDLAAAAGLSRAHFSRM
                     FTRTFGESPRSYLQSRRLERAAALLRYTDRSVADICVMVGLQSVGSFTTSFARVYGLP
                     PAAYRASMPPAAIYARVPSCILRRDTRRPADSRVKTAHGEKTNGSGRP"
     gene            8548..8972
                     /gene="rnpB"
                     /locus_tag="VFC01_05205"
     ncRNA           8548..8972
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFC01_05205"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(8982..10472)
                     /locus_tag="VFC01_05210"
     CDS             complement(8982..10472)
                     /locus_tag="VFC01_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HZN79076.1"
                     /translation="MAKSNDPATSRYLEVERKFEVLESTVSPSFEGLSSVARVERSPS
                     QQLDAVYFDTAAHDLAARRVTLRRRTGGTDAGWHLKLPAGPDARTEVRVPLDESNVDT
                     VPEDLLDVVLAIVRDRPVGPVARISTRRTVDMLYGPDGVAVAEFCDDDVVARADDDEE
                     SEQRWREWELELGEGADRALLDRLTNRLLDAGAVTAGHGSKLARVLERDDTDTAIEPP
                     ADPVHRAVAEQVEQLVVWDRAVRADVYDSVHQMRVITRKIRSLLQASEGAFGISDDAW
                     ILDELRQLAAVLGVARDAEVLAERYERAIDELPAELVRGPVRERLVDGAKKHYKSGLR
                     RSLIAMRSERYFRLLDALEGLVAAEPPPTPPGEEPAQLTIDSAYRRVRKAAKAAAQAA
                     DTEKDEALHRIRKGAKRLRYTAAATGEGKVSERAKTIQTLLGDHQDSVVSRTHLSRQA
                     EAAQAAGEDTFTYGLLYQLEDNLARRSREQLDDALKKLDRSVRKAR"
     gene            complement(10510..12504)
                     /locus_tag="VFC01_05215"
     CDS             complement(10510..12504)
                     /locus_tag="VFC01_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HZN79077.1"
                     /translation="MTAIEVVRPGMLTTVQDWPGRIGYWHVGVPPSGPMDDLSFRIGN
                     RVLGNLEGIAGLECTRAGPALRFPDGGRVCITGAPVSVTLDGTPVPQWQSIGVPVGGL
                     LDVGALTGPGMRCYVLIAGGLQELEYLGSTATFTLGAFGGHLGRPLRDGDVLTADVDP
                     GPADGRPARAAIDEQPSIGHRWEVAVTEGPHAAPEFFTRADIEALIRTDYAVHFNSDR
                     TGVRLMGPQPQWARSDGGEAGLHPSNIHDNAYCVGTLDFTGDTPILLGPDGPSLGGFV
                     CPVTVVGGDRWKLGQMAPGDAVRFVPVRADRAPSLRTIDIDRRASFPLVISTSSDNDD
                     GVLSRFTAADGTDVVLRRDGDGGVLVEYGPMVLDLAMRARVHVLYEHLLAQCIPGITE
                     LTPGVRSLQVQFDPAVLSMAEMTDLLAGLDSELPSSDQLVVPSRTVRLPLSWDDPATH
                     EAIERYMHGVRADAPWCPWNIEFIRRINGLDDVAQVHDIVFAAEYLVLGLGDVYLGAP
                     VATPLDPRHRLVTTKYNPARTWTPENAVGIGGAYLCIYGMEGPGGYQFVGRTTQVWNH
                     CHPGGARSFEPGTPWLLRYFDRISFYPVSAEELVHLRAEMAAGRGEVAIDDGKFSLAD
                     YRDFLTDNDDHITAFRQQQAVAFAAERRAWDLAGEFRRAS"
     gene            complement(12504..13133)
                     /locus_tag="VFC01_05220"
     CDS             complement(12504..13133)
                     /locus_tag="VFC01_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP2"
                     /protein_id="HZN79078.1"
                     /translation="MTTTELTIIGDEVVDARAPWSAIVRTGQTLQIIDLHGNQAVDTL
                     LYGVSGDQVDHALRYSAQATITAQRNIFLSTGSVLKAANGTPLMTIVADDVGNHDTIA
                     GACSKESNTLRYGHHTVHQHACAENFLAEGAKWGLGKRDIVPNINFFMNVPVEADGTL
                     GIVDGLSAPGKSLTLRAERDTLVLVSNCPQINNPCNGFDPTPVRMVISA"
     gene            complement(13130..13879)
                     /locus_tag="VFC01_05225"
     CDS             complement(13130..13879)
                     /locus_tag="VFC01_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019932733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HZN79079.1"
                     /translation="MSTDSTTGARDHARSQVQTATMRIPATPTDVDPSRLLWAEAVPP
                     GSYATRVLGRGTRLRLIDPDGGACAHLLLFRADAPWERLNVADTVKVPWQAYLGAGHP
                     LLSDQGRVLATVTDSSGQHDTLCGMTDAGRAKMLLAAVKHGLDLRDVAPSVSFFKGVR
                     VGDDGTLGFTGSAGPGSAVDLLIHLPVVVALVNTAHPLDPDPALTGLDVLAWRAGEQL
                     ATPVSDDPEYLRALFNTESTWAAAQTSETFT"
     gene            complement(13952..14329)
                     /locus_tag="VFC01_05230"
     CDS             complement(13952..14329)
                     /locus_tag="VFC01_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79080.1"
                     /translation="MISTAKIVVTLTAGIAATFVAAELQVNERNPWEKSAALLLIPML
                     CATFWVLWLRAPSHDKEIGVDELRTAKSVADRAHWLMLAQVAFSVLSGLFAVLGLLIP
                     SDTGQASPGPNTCHLREIQSTHS"
     gene            14429..15097
                     /locus_tag="VFC01_05235"
     CDS             14429..15097
                     /locus_tag="VFC01_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN79081.1"
                     /translation="MRSDGRGRPRLEQSRRPGKSAREEILDAAAELFTNLGYASTSTR
                     RIADAVGVRQASLYHHFATKDDILDALLAGTVDEPLELAAELLDEEGPAVQRLHALVV
                     GDVTQLCASRWNLGALYLLPELRLDRFEQFRLRRAELRARYRTLAAEVIEECDGPVDA
                     DDLPFRLVESVINRRSDDTDCPDEQPWVIADGALRTLGFDGGFAKLHKLTAARLGFAD
                     TVQV"
     gene            15094..15873
                     /locus_tag="VFC01_05240"
     CDS             15094..15873
                     /locus_tag="VFC01_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN79082.1"
                     /translation="MTEYQTITFEQSGPITRITLDRPDAANGMNDTMTRELADAAKRC
                     DSPATKVVVITGSGRFFCAGGDLKSFAAAETRGPFIKGVADDLHRAISTFARMDAVVI
                     TSVNGVAAGAGFSLAVTGDLVLAAESASFTMAYTRAGLSPDGSSSYFLPRLIGITKTK
                     ELMLTNRTLTAPEASQWGLVTEVVPDAELAARTDKLAHQMAATARGSNGGVKALLLST
                     FANGLEEQMELEGRLIAGLADSVDGREGIDAFLAKRKAEFA"
     gene            complement(15870..16706)
                     /gene="panB"
                     /locus_tag="VFC01_05245"
     CDS             complement(15870..16706)
                     /gene="panB"
                     /locus_tag="VFC01_05245"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZN79083.1"
                     /translation="MSEQSVYGAAEVKPRGKVRTHHLQKWKAEGHKWAMLTAYDYSTA
                     RIFDDAEIPVLLVGDSAANVVYGYDTTVPVTIDELIPLVRGVVRGAPHALVVADMPFG
                     SYEAGPQQALATATRFLKETGAHAVKLEGGERVADQIATITQAGIPVVAHIGFTPQSV
                     NGLGGFRVQGRGDAAEQTIHDAIAVQEAGAIAVVLEMVPAELATQITGKLTIPTVGIG
                     AGPNCDAQVLVWQDMAGFTSGKTAKFVKRFGDVGSELRSAATQYADEVASGAFPAEEH
                     SF"
     gene            16850..18241
                     /locus_tag="VFC01_05250"
     CDS             16850..18241
                     /locus_tag="VFC01_05250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZN79084.1"
                     /translation="MQRLSGLDASFLYLETAKQPMHVCSILELDTSTMPGGYTFDRLR
                     DALTLRIQAMPQFREKLADSRFNPDHPVWVDDKDFDVDRHLHRIGLPSPGGRRELAEI
                     CGHIASLPLDRSRPLWETWVIENVAGTDAHAGGRIAVMTKMHHAGIDGVTGANLMSQL
                     CSTEPDAPAPDPVEGIGDASGLEIAVSGAVKFVTRPLRLVNVVPTTLTSVVDTVKRAR
                     SGLAMAAPFAAPKTAFNTNVTGHRNIAFAQLDLEDIKTVKNHFGVKVNDVVMALVSGV
                     LRKFLADRGELPDNSLVAMVPVSVHEKSDRPGRNQVSGMFSKLETHVDDPAERLKSIA
                     ESNSVAKQHSSAIGATLLQDWTQFAAPAVFGVAMRVYAASRLSGARPVHNLVISNVPG
                     PQEPLYFLGNEIKAMYPLGPIFHGSGLNITVMSLTGKLDVGIVSCPELLPDLWDLADD
                     FEVALNELLDATR"
     gene            18287..19795
                     /locus_tag="VFC01_05255"
     CDS             18287..19795
                     /locus_tag="VFC01_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN79085.1"
                     /translation="MNLRIGVLATIAALLLAGCSNMVDGRAVISAPRPGSPIQWLPCK
                     AAASDQNRIPAGAECGMLSVPVDYSKPDGDVAQIAMIRFKATGQKVGSLVVNPGGPGE
                     SGVEAAASMAPTLPQSVRERFDLVGFDPRGVANSTPAAWCNSDEDNDKLRADPTVEYT
                     QEGVDHIEKENKEFVQRCVDKMGKEFLANLGTANVAKDLEAIRTGLGDDKLTYLGYSY
                     GTRIGALYAEAYPDKVRAMILDGAVDPNADQIEEEIRQAAAFQKAFDNYAADCASSPD
                     CPLGTDPSKAVDVYKSLVDPLVKHPAETKDPRGLSYSDAIVGTILPLYSPSLWRHLTQ
                     ALSEIKEGRGDTMLAMADLYMGRDDKGHYNNSTDVRVAVNCMDKPHITDRAKVVDEDR
                     RTREVAPFLSYGEFTGLAPLDTCAFWPVPATGDQHELKVAGLPPILVVSTTNDPATPY
                     QAGVDLAQQLGGTLLTFDGTQHTVVFQGNTCVDDIAARYLVDVTVPPPNTRC"
     gene            19789..20238
                     /locus_tag="VFC01_05260"
     CDS             19789..20238
                     /locus_tag="VFC01_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN79086.1"
                     /translation="MLTITTVGSADVPDLMPMLRAYCDFYRVDPPDDQLAALVTALID
                     DPSEGLQLIARDTDGTPLGFATIYWTWQTLYAARVGVLNDLFVVPASRGSGAGRALIE
                     RCRELCREHGAGKLVWETAPDNATAQRLYDGIGAEKSTWLTYELDAR"
     gene            20295..>21291
                     /locus_tag="VFC01_05265"
     CDS             20295..>21291
                     /locus_tag="VFC01_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN79087.1"
                     /translation="MLAMWRVGRWSTVLLAIFLMSAIACPAAWASPEGESIQQYGQPP
                     LWGSCEQFIGDATRIPTAQCGTVSVPVDYAKPEGAQAQLAVIRIPASGDRIGVLVVNP
                     GGPGASAVDTVASMGASLADTDIMRRFDLVGVDPRGVGHSTPELRCRTDAEFDAFRRE
                     PMADYSPEGVAHIEAVWRQVVDACAQRMGSEFLANVGTASSARDMDVVRAALGENQIN
                     YLGFSYGTELGAAYAEQYPDRVRAMVLDGAVDPSLDPITNSIRQMAGFQTAFNDYAAD
                     CAQSAGCPLGTDPAKFVDRFHELVDPLVNKPGWTSDPRGLSYQDAITGTVNALYTR"
BASE COUNT         3702 a         6933 c         7065 g         3591 t
ORIGIN      
        1 ctcggtgggc gccggtttgg acaaatggat tgtcttcgta cccgccgaca gcgccgacct
       61 ggtgcgggac gcgatgttcg cggcgggtgc gggcagcgtc ggcaccgtcg agcgggtgcc
      121 cgcggaccgt gtcgaggtga ctgcgccgac acggctgagg gcgcatgtgc tggcagcgat
      181 gcgcgccgcg catcccaatg aggagcccgc gttcgacatc tatgcgttgg ccactgtccc
      241 cgccggtgtc gggctgggcc gagtcggttt gttgccgaga gcggaaccgt tgtccgcctt
      301 cgtttcccgc gtgcacgatg cgctgcccgg aacctcgtgg ggcgtgcggg cctcggggga
      361 ctcggaggcg atggtgtctc gggttgcggt atgcggggga gcgggcgact cgctgctcga
      421 cacggttgcg gacgcgggtg tgcaggccta cgtcacagcc gatctgcggc atcaccccgc
      481 tgacgagcac aggcgcgcat cggatgtcgc gctggtcgac gtcgcgcact gggccagcga
      541 atacccctgg tgtacccagg cggccgatgt gctgcgaagc catttcggcg acgcgctgcc
      601 ggtgcgggtg tcggcaatac gcactgatcc gtggaatgtc gagagaaacg agacatgaaa
      661 gctgaagtag ttcaacagcg ttcgctgctc gagctagcgg agctcgatgc agagctgagc
      721 cgcatcgagc accgggccaa gaatctgccg gcgcagcagc aactcgagga ggcgcagacc
      781 gcgcaccgtg aagcgaatga ccgcctggcc gcggtgcaga tcgcgctcga agaccttgac
      841 gcgcaggtgg ccaagttcga gtccgagatc gacgcggttc gacagcgtga ggaccgcgac
      901 cggtcgatgc tgtcgtcggg caccgtggac gccaagcagc tcaccgactt gcagcatgag
      961 ctggagacgt tggagcggcg ccagtccagc ctggaggatt cgcttctgga ggtcatggag
     1021 cgccgcgagg agctgcaagg tcagcaggcc gccaaacttg ccgagatcga cgagctgcag
     1081 agcaagctga ccgaagccca acgggcgtgc gacgaggccc ggacggagat cgatcagtcg
     1141 cgccatcaat ccatctcgag gcgagacgaa ctcgtcgcct cgttggacgc cgacctcgtt
     1201 gcgctctacg aacgtcaacg ctcccgcggc ggtgcgggcg ccggcctgct gcaggggcgg
     1261 cggtgtgggg cctgtcggat cgagatcgac cagggtgagc gggcacggat cgccgcagcg
     1321 gccgaggacg acgtgttgcg atgccctgag tgtggggcaa ttctgttgcg ggtcaagggg
     1381 accggcgcgt gaaggtcatt gtcgaggcgg acggcgggtc gcgcggtaac ccgggacctg
     1441 ccggatatgg gacggtggtg tggacggcgg accacggcac cgtgctggcg gaaagcaagc
     1501 aggcgatcgg acgggccacc aacaacgcag ccgaataccg cgggctgctt gcgggactgg
     1561 aggaggccgt caagctcggc gccaccgacg tcgacgtgat gatggactcc aagcttgtgg
     1621 tggaacagat gtcgggccgc tggaaggtca agcatcccga tatcgccgcg ctgcatcaac
     1681 aggccacggc gttatccggg cggttcgagc acatcagcta cacgtggatc ccgcgtgcca
     1741 agaacagcca cgccgaccgg ctggccaacg aggcgatgga tgccgccgcc gaaatggaca
     1801 ctgccgcaga ggcacccaag gaatcgtcga cgccgtcgtc agcccccgga tggaccggtg
     1861 cccgcggcgc gcccacccgg ttcttgttgc ttcgccacgg gcaaactgag ttgtccacgc
     1921 agcggcggta ttccggccgt ggcaatcccg cgctgacgga cgtcggacgg cggcaagccg
     1981 aggccgcggc acaattcctc gcgcagaagg gcggcatcga ggccgtcatc acctcaccgc
     2041 tgcagcgcgc ttacgacacc gcggcggcgg cggcgaaggc cctcggcctc gacgtcaccg
     2101 tcgaccacga cctgatcgaa acggacttcg gggcgtggga agggctgacc ttcggtgagg
     2161 ctgccgagcg cgacccagat cagcatcggc gatggctgcg cgacaccagc gtgacgccgc
     2221 ccgacgggga gagtttcgac agcgtggccg aacgcgttcg tcgggcccag gcccgaatca
     2281 tcaacgaaca cacgggccag aacgtgttgg tggtgtcgca cgtgaccccg atcaagacgc
     2341 tgctccggat ggcgttggac gccggcccgg gcatccttta ccgattgcac ctcgaccttg
     2401 catcgctttc catcgcggag ttctatccgg acgacgtggc gtcggtgcga ctggtgaatc
     2461 aaacggccta cctgtaatca gttgtgcagg tggagccgtt cgccgtgcac cccgaacagt
     2521 gtgatcgcct cgacgggccc gtcgaccgcc ccgaaccagt gcggggtcca cgtcgaaaac
     2581 tccacggcct caccgggctt gatgacgaag tccttctccc cgaggatgag cctcatccgg
     2641 ccggagagta cgtacatcca gtcctggcct tcatgcaccg gcagttcggc aggcgggtta
     2701 cgccgacggg cgctgactcg gatcttgtac gcatgcaatc cgcccgccgg gccgtggcgg
     2761 gtcagtggcc agtaggtcac gtcgttgtgc gtgtgcgatc cgcccttgac gcgcgggtcc
     2821 tgcgcctcgg gcgcccgcag gagttcgtcg gtgcttaccg acagcgctgc ggctagtcgg
     2881 gggaggtggt cgagtgcgag ccggcgcttg ccggattcca gcctgctcaa ggtggacacg
     2941 tcgatgctcg accggctcgc gacatcctcc agcgtcaatc cgcgctgcat acgcagttcc
     3001 ctaagccggc gacggacccg aaggtcgacg ccatcctcgg actttgcctc catggcaaat
     3061 cagcttggca ggcggagctg ggcgcgtgca agctcgagac atgaacacga ctgatgccaa
     3121 ggagcactgg gagcagcact acggcgagcg agaccgtcgt tggagcggtc gcgttaacgt
     3181 ccgcctgcca gaggtggccg cggagcttcg acccggcact gcactggatc tgggctgcgg
     3241 tgaaggcgcc gacgcgtggt ggcttgccga gcgtggctgg accgttgtcg ccgtcgacat
     3301 ttcggacacg gcgttgcagc gcgccgctac cgaagccgac gctcgagggc tgagtgaccg
     3361 gatcgagttc gtccagcagg acctatcgga tggattcccg tccggcacct ttgatctgat
     3421 ttcggcgcag tttctgcatt ccatgattcc gttcgatcga ccgcgggtgc tcaagcgcgc
     3481 ggcaggcgcc gttcgccccg gtgggtcgtt gctgatcgtt gaccacgctg ggccgccgcc
     3541 gtgggcgtcg aagctcgatc atcaccacga gttcccaagc gcggaggaag ttatcggctc
     3601 actcgaactc gacgatttcg agtgggagcg ggtccgtgtg gaagccgtcg agcgagaggt
     3661 gaccacaccg gacgggcaga ccgcgacctg gttcgacaac gtgatcgttc tgcgtcggcg
     3721 ctaggtgcgg gccttccacg ctcggtaccg ctcgggcagg catctggcac acggtcggta
     3781 gcccgcggcg atggcggtgg cctcgtcggc gaagaactcg cgatgggtga cgtagccgcc
     3841 tttggcgagc gcccggagcg ccgacggaca gtccagccgg ccgtagatcc gcgtcgcttt
     3901 gtgtcctccg agcgtgcccg gtgtggccga gtcgatcggt atgccgttgg agccgattag
     3961 tgtgtagtgc ttcatatttc aggcggcgtc gtgaaagacc agcccgagtg taaagcgctc
     4021 accggagcgc acggccgaga cgccgtgccg gaccggcgat gccgaccagc ctctggtcga
     4081 ttccacaggc cgttcccgcg tcgtgaagac gaagccgtga ccgaacggaa gcagcgttgc
     4141 ggtgccgcgc gattgtgcgc gcggacgctg ctcgacgagc aggaactcgc caccggtgtg
     4201 gtcaaccccg ggttcgctga ggttgatcac gacttgcagc ggaaacacca agtcgccgta
     4261 tagatcccga tgcagcgcgt tccagtcgcc ctcgccgtat ttcagcagga tcgcggtcga
     4321 cttcttctgg cccgcatcgt ggcacatgtc cagccaatcg tcgaggctgt cgggccaggg
     4381 cgccttgcgg cccagcttgg tccaccagtc ccgcgcgatc ggtaacagtc gcgggtatat
     4441 cgcctgcttg agctgttcga tgggctcggg ataaggctgc tggaagtacc ggtactcgcc
     4501 gtcgccgaat cggtatctgc gcatgtcgat cgtcgcgcgg aacaggtggt cgtccgggta
     4561 gagcccgcgg atcctctccg cctcggcctt ggtgagcagc cggggaagca gtgcaccgcc
     4621 aaagtcgttg acttcggcgg tgattgcgtc ccagtcacca gagtcgacac gtttctgcca
     4681 tttcgtctga gccatcagaa caacctcgcc gccttcactt cgcttggctc ttccaattcc
     4741 agcagtagtt gcttgcggtt gagcccgccc gcgtatccgg tcagctggcc gttctttccg
     4801 accacccgat ggcacgggac gatgatgctc agtgggttac ggccgactgc ctgcccgacc
     4861 tcccgggcgg tggtgccgtc cgccactgcg tcagccaatt ccccgtacgt gatcgtctcg
     4921 ccgtacggga tggtgctcag taggtgccag acacgccgct gggcttcgtc gccgttcagc
     4981 ttggttggca ggttgaagcc gtgccggcgg ccggcaaggt agtccgtcag ctgggtctgt
     5041 gcctccgcga gtagcccgtc ggatttggct tcgacccgct cgccgagcga ttcggcggcc
     5101 ggtcgatacc agtggtgccg gaaatagagg ccggtcagcg cgtcgtcctc ggcgaccagg
     5161 gtgagctcgc cgagcgggct ttcgattacc gcatgtcggg tgttcatacc ttgtagacga
     5221 ggcgggggat cggcgtgtga gatttgtcgt agggccatgg catactcgaa cacatgttcg
     5281 aagttgtggc ggatgctgat ctcatcgatg tgatgagtga ggcgaccagg gatgagtcca
     5341 cggcggccga cgcatccgtc gaacaacaag ccgtattgcc gctgtcagct caggttcctc
     5401 cgttctacag agcggcagcg cttgagagag ggaagatgac cgacatgggt gaggcgaagg
     5461 gcccgttcgg cgggtcgtcc cactcctcgg cgagcgccga ggatgtatgg gcggtatgga
     5521 cgaacccgtc cgagtggcca ggagatgtca ttgcgatggc caagatcgac ggcccgttcg
     5581 cggtaggggc gaagatcacc actcgggtca agggctaccc gccgctgaca tcgaccgtca
     5641 cccgaatcga accgccgatg ttgtggacgg gagtggcgaa agcgccggga ttgacgcaga
     5701 ccatcgatca cctcatcgag cccactgact cgggcacttt gatcacagag cgcaccgtct
     5761 ttaccgggcc gctggcttcg atagccgcgc gtttagtcgg cggaagactg cggaagactt
     5821 ttgaggcgac gacggcacac tgcgggcggc tggccgagag ccgcgcgtcc cactagagcg
     5881 ttgatacgca gcaagcacaa atagccgtcg cccggctcac accagagtgt tcttcaccca
     5941 atcggtgtca tcacctgatc aagaccttct tcggcgggcc gggtggttgg acggatcagc
     6001 agctgcccga cggcactatt atcttgaccg cacccaccgg gcacgcctac accaccgagg
     6061 cacacggcgc ccacatgttt cccgcacttg gacaatccac gggtgaggtg gacatcccgc
     6121 cgcatttgat gccgccagat accgatcggt cggtgatgat gccgcgccgc aagcaaactc
     6181 gcgagcagga tcgccgcgac cgcataaatg ccgaacggcg cgaacgcacc gaactcatcg
     6241 ccgaagaaga acgccaacgc caagcctggc tcgccgccaa ctacgaacca ccgccgttct
     6301 gactgcagct agttgtattc gactctgccg cagtcggttt cgacagtgcg gcagccctgg
     6361 gtggacatag cgcccatgag gcaacggtca acgcgagcag cgccagtggt gcaatcaacg
     6421 gtgcgggcgt gccgtcgacg attacgtggg atgcgacggc ggaagcataa ttgatggtcg
     6481 ctccggcgta cgcccattgc ttgaggcgcg ggaaccgggg aatcagcagc acgactgcgc
     6541 ctgcgagctt ccacacgccc atgatcgtca acagatatgg cggatagcca agatgctcga
     6601 tgacgccgcg gacgtagtcg atccgcagca ggtcccagat cccaccgacg gcgagttccg
     6661 cggcaaccaa tgccgtcgtg acccaatagg cggcgaagcg gccgggcctg gatgtgccgg
     6721 tggcaggggg gatctgacga cgcgcaggtg gccgcagcca ccacgatgcg gcggtgagga
     6781 cgagaaagac cgtcggcccc agaagcgttt cagcgtcgtc gccgaccgcg atgtgcgatg
     6841 ccgcggcgcc ggtgtaattg atgacgaggc ccgcgtacgc ccactccttg agccggggta
     6901 gccctggcgc caacacgatc aagccggcag atatgtacca gacaccgaga atcgacatga
     6961 agtacacggg ataacccagc tgcgttgccg tgtcgcgaaa cggtcgcagc tgcagggcgc
     7021 ccattactcc gccgccgatg cattcggcgg caacgatgag ggttgaggtc caatacgcgg
     7081 cggaacggta catacagatg tgacgaaccg gccgccacga atgtgaccgc tatccctgcg
     7141 ccttccacgg tgtcaaccgt ggagtccacg caggcacgtt gcggcggtac tcctcgtact
     7201 gctcgccgta tgtgcgaacc aacgtcggct cttcatacca gtgcacgaac gaggccgtcg
     7261 cgatccagcc gatcactgcg tagagcacca gccaggggct gtcgaacagc agcgcctgac
     7321 cgaggatcgc cgtcagcagg cccacgtaca tcgggttgcg cacgaatcgg ttgaacccgg
     7381 tgacgacgag acgctccgtg ggcgccactg gcatcggcgt gccgccggcc ttggcgaact
     7441 ggacgaacgc gtggactggc gggataaggc ccaagacgat caacacgacg ccgaggacca
     7501 cacgcaaagg tgggctggac cccgggatct cccagtgggt gatcaaccag gggcccagcc
     7561 caacgaaagt tccgggggcc accagaaaga aggccgccga cccgaccgct gcggtggaag
     7621 ttcgcaccta tccagtattg gccgggatct ccgtcaattg ggtcagtcgc acactgttgc
     7681 ccgacggatc gcggaatccg cagtcgatgc cgtacggcat ctggtgtggc tcctcggtga
     7741 attcgacgcc gcgggccttc atttcctcac agctggcctg gcagtcctcg gtggtgagga
     7801 agacggtgcc ggcgaagccc ttcgcggtga ggttgagcac ctccttgcgt gtcgcttcat
     7861 ccatcaccgg ctcgccgggc accgccatca gcacgatcga aacgtcgtcc tggccgcgcg
     7921 ggccaaccgt cagccaccga aattcgccca tctcctccgg aaacgaaaca tcttggcgca
     7981 cttccattcc gaccttctcg gtccagtact tcagcgcaac ttcttggtcg tgtacccaca
     8041 ggtgtgagct tgcaattttg atcatgtgca cgacgctacg gtcggccgct gccgttcgtc
     8101 ttctccccgt gtgctgtctt gacgcggctg tcggcgggac gacgggtgtc gcgccgcaag
     8161 atgcaactcg gcacccgcgc gtagatcgcg gcgggcggca tgctggcccg ataggcggcg
     8221 ggcggcaacc catagacccg cgcgaagctg gtggtgaacg acccgacgct ctgcaggccc
     8281 accattacgc agatgtccgc caccgagcgg tcggtgtagc gcagcagcgc cgcggcccgc
     8341 tcgaggcggc gcgactgcag atacgagcgc ggcgattcgc cgaaggtccg ggtgaacatc
     8401 ctgctgaagt gggcccgcga cagtccggcc gctgcggcca ggtcgtcgac ggtgatggcc
     8461 tcggcgtagc gggcgtccac gagatccttg gcccgctgca ggtagcggac aggcggaacc
     8521 tgcgtcatgc gatcattatg aggcggatga gttggctggg cggccgcgga tcatgcgcca
     8581 aaccgtgggg gattaggtgc ggcggtggtt cgaggaaagt ccggacttca cagagcaggg
     8641 tgattgctaa cggcaatccg aggtgactcg cgggaaagtg ccacagaaaa tagaccgcca
     8701 ccttcgcggt ggtaagggtg aaacggtgcg gtaagagcgc accagcattc cgggtgaccg
     8761 gagtggctag gcaaacccca cccgaagcaa ggccaagaat gtcgcagagg gggtgcggca
     8821 gcgcaggcgt ccgagggctg ctcgcccgag cctgcgggta ggccgcttga ggcacccggc
     8881 aacggtgtgt ccagatggat ggtcgccgcc tcgtcgccgc ggtagtacgc ggcggcgagg
     8941 gacagaatcc ggcttacagg ccaactcatc cgcccgctcg gttatcgcgc tttgcgtacc
     9001 gaccggtcca gcttcttcaa cgcgtcgtcg agctgctcgc gggagcgccg cgccaggttg
     9061 tcctccaatt ggtagagcag cccataggta aaggtgtcct cgcctgcggc ctgcgcggcc
     9121 tcggcctggc ggctcagatg ggtacggctg accacactgt cctgatggtc accgagcagc
     9181 gtctggatcg tcttggcgcg ctcgctaacc ttgccctcgc ccgtcgccgc cgctgtatac
     9241 cgaagtcgct tggcgccctt acggattcgg tgtagcgcct catccttctc ggtgtcggcg
     9301 gcctgcgctg cggccttggc cgccttgcgt acccgtctgt aggccgaatc gatggtcagc
     9361 tgggccggct cttcacccgg cggtgtgggc ggcggctccg cggctaccag cccctccagc
     9421 gcatccagca gtcggaagta gcgctctgac cgcatcgcaa tcagcgatcg ccgcaggccg
     9481 gacttgtaat gcttcttcgc gccgtcgacc aaccgctccc gcactggccc tcggaccagt
     9541 tcggcgggca gttcgtcgat cgccctttcg tagcgctcgg cgagcacctc agcgtcgcgg
     9601 gccacaccca gaaccgccgc cagctgccgc agctcgtcga ggatccacgc gtcatcggag
     9661 atcccgaacg caccctccga tgcctgcaac aggcttcgga tcttgcgggt gatcacccgc
     9721 atctggtgca ccgagtcgta cacgtcggcc cgcacggcgc ggtcccacac cacgagttgt
     9781 tcgacctgct cggccactgc gcggtgcacc gggtcggccg gcggctcgat cgcggtatcg
     9841 gtgtcatcgc gctccagtac ccgcgcgagc ttcgaaccgt ggccggccgt gaccgcgccc
     9901 gcgtccagca gccggttcgt cagccggtcc agcagcgcgc gatcggcgcc ctcgcccagt
     9961 tccagttccc actcgcgcca ccgttgctcg gattcctcgt cgtcgtcggc tcgcgcgacg
    10021 acgtcgtcgt cgcagaactc cgccaccgcg acgccgtctg gaccgtagag catgtcgacc
    10081 gtgcgcctgg tcgagatccg cgcgaccggg ccgacggggc gatcacgcac gatggccaat
    10141 acgacgtcaa gcaagtcctc cggcaccgta tcgacgttgc tctcgtcgag cggcactctc
    10201 acttcggtcc tggcgtccgg ccccgcgggc aacttgaggt gccaacccgc gtcggtgccg
    10261 ccggtgcgac ggcgcagggt gacgcgccgg gccgcgagat cgtgggccgc tgtgtcgaag
    10321 tagacggcat cgagctgctg ggacggcgaa cgctcgacgc gcgcgacgga ggacagcccc
    10381 tcgaacgacg ggcttacggt cgattccaac acctcaaact tgcgctcgac ctccaggtac
    10441 ctggacgtcg cggggtcatt ggatttcgcc attgtgcagc acagcgtgcc atacccaggt
    10501 gaacgagcgt caggacgccc ggcggaactc gccggccagg tcccacgccc gacgttcggc
    10561 cgcgaacgcg accgcctgct gttgacggaa cgcggtgatg tggtcgtcgt tgtcggtcag
    10621 gaagtcgcga tagtcggcga gcgagaactt tccgtcgtcg atcgcgacct caccgcgccc
    10681 ggcggccatc tcggcgcgta gatgcacaag ctcctccgca ctcacggggt agaagctgat
    10741 tcggtcgaaa tatcgcaaca gccaaggcgt tccgggctcg aacgatcggg caccgccggg
    10801 gtggcagtga ttccacacct gcgtggtgcg gccgacaaac tggtatccgc cggggccctc
    10861 cataccgtag atgcacaggt aggcgccgcc gatgccgacg gcgttctccg gcgtccaggt
    10921 gcgcgccggg ttgtacttcg tggtcaccag ccggtgtcgc ggatccaggg gagtggcgac
    10981 gggtgcaccc aggtacacgt cgccaagccc gaggaccaaa tactctgcgg cgaacacgat
    11041 gtcgtgcacc tgcgcgacgt cgtcgagacc gttgatccgc cggatgaatt cgatgttcca
    11101 tgggcaccac ggcgcatccg cccgcacacc gtgcatgtag cgctcaatgg cctcgtgagt
    11161 cgccgggtcg tcccacgaca atggcagtcg gacagtgcgg ctcggcacga cgagctgatc
    11221 ggacgagggc agttcgctgt cgagaccagc cagcaagtcg gtcatttcag ccatcgacag
    11281 caccgcgggg tcgaactgca cctgcaggga gcgcacgcct ggggtgagct cggtgatgcc
    11341 gggaatgcat tgtgcgagga ggtgctcgta gagcacgtga accctggccc gcatggccaa
    11401 atcgagcacc atcggcccgt actcgaccag cacgccgccg tcaccgtcgc gccgcaacac
    11461 cacgtcggtg ccgtccgccg cggtgaaccg gctgagcacg ccgtcgtcgt tgtcgctgga
    11521 cgtcgagatc accagaggga acgacgcccg tcggtcgatg tcgatggtcc gcagcgatgg
    11581 cgcccgatcg gcgcgcaccg gaacgaaccg caccgcgtcg cccggtgcca tctggcccag
    11641 cttccagcgg tcgccgccga ccacggttac cgggcagacg aacccgccca ggcttgggcc
    11701 gtcagggcct agcaggatcg gggtgtcgcc ggtgaagtcc aaagtgccga cgcagtaagc
    11761 gttgtcgtga atgttcgacg ggtgcagccc ggcctcaccg ccgtcgctgc gcgcccactg
    11821 cggttgcggt cccatcagcc gcacgccggt gcgatccgaa ttgaagtgca cggcgtagtc
    11881 ggtgcggatc agcgcctcga tatcggcacg ggtgaagaac tcgggtgcgg cgtgcgggcc
    11941 ttcggtgacc gcgacctccc agcggtggcc gatgctgggt tgctcgtcga tcgccgctcg
    12001 cgcgggacgg ccgtcggctg gtccagggtc gacatccgcc gtcagcacat cgccatccct
    12061 caacggcctg ccgaggtgac caccgaacgc gccaagtgtg aatgtggctg tgctgccgag
    12121 gtattcgagt tcctgcaggc cgcccgcgat cagcacgtag cagcgcatac ccggacctgt
    12181 cagcgcccca acgtccagca gtccaccgac tgggacaccg atggactgcc attgcggcac
    12241 gggcgtgccg tccagtgtca cgctcaccgg cgccccggtg atgcagacgc gtccgccgtc
    12301 cggaaagcga agggccggac cggctctcgt gcattcaaga ccggcgatgc cctccaggtt
    12361 gccgaggacc cggttgccga tccggaacga caggtcgtcc atcggcccgg acggcgggac
    12421 accgacatgc cagtacccga tgcgaccggg ccagtcctgc acggtggtca gcattcccgg
    12481 ccgcaccacc tcgatcgcgg tcattacgcc gagatcacca tccgcacggg tgtcgggtcg
    12541 aatccgttgc aggggttgtt gatctgcggg cagttcgaca ccagcacgag cgtgtcacgc
    12601 tcggcgcgca acgtcagcga cttgccaggt gcggagagac cgtcgacgat gcccagcgtg
    12661 ccgtcggctt cgacgggcac gttcatgaag aagttgatgt tcggcacgat gtcgcgtttg
    12721 cccagccccc acttggctcc ttcggccaaa aagttttcag cacaagcgtg ttggtgcacg
    12781 gtgtgatggc catagcgcag cgtgttcgac tccttcgagc aagcacccgc aatcgtgtcg
    12841 tggttgccga cgtcgtcggc gacgatcgtc atcagcggtg tgccgttggc ggccttcagc
    12901 accgacccgg tggacagaaa gatgttgcgc tgcgcggtga tggtggcctg cgcgctgtag
    12961 cgaagcgcgt gatcgacctg atcgcccgaa accccgtaca gcagggtgtc gacggcttga
    13021 ttgccgtgca ggtcgataat ctgcaacgtc tggccggttc gtacgatcgc cgaccacggc
    13081 gcccgggcat cgacgacctc gtcgccgatg atggtgagtt ctgtggtggt catgtgaagg
    13141 tctcgctggt ttgcgctgcg gcccaagtgc tttcggtgtt gaacagggcg cgcaggtatt
    13201 caggatcgtc gcttacgggc gtggcgagct gctcgcccgc gcgccaggcc agcacgtcga
    13261 ggccggtgag cgccgggtcg gggtccaacg gatgagcggt gttgaccagc gccaccacca
    13321 cgggtaggtg gatcagcaga tcgaccgcgg accccggccc ggcagaaccg gtgaatccca
    13381 atgtgccgtc gtccccgacg cggacaccct tgaagaagga caccgacggt gcgacgtcgc
    13441 ggagatcgag gccgtgtttg acagccgcca gcagcatctt ggcgcgcccg gcatcggtca
    13501 tgccgcacaa cgtgtcgtgc tggccggaac tgtccgtgac ggtcgccagc acgcggccct
    13561 gatcggagag cagcggatgg ccggcgccga ggtatgcctg ccaaggcacc ttcacggtgt
    13621 cggccacgtt gagtcgctcc caaggcgcat cggctcggaa gagcagcagg tgcgcgcacg
    13681 cgccgccatc gggatcgatc agccgaaggc gtgttccgcg ccccagcacc cttgtcgcat
    13741 aggagccagg gggaacggct tcggcccaca gcaggcgcga cggatcaacg tcggtcggcg
    13801 tggcgggaat ccgcatcgtg gcggtttgta cctgagaccg ggcgtggtcg cgcgccccag
    13861 tggtcgagtc ggtactcaag gtcggctcct gtccgaataa ctgtcaactg aaagttttca
    13921 gcgcaccgat tccacccgcg tgtcgcggtt gttacgagtg cgttgactgt atttctctca
    13981 agtgacaggt attcggtcca ggcgatgctt ggccggtgtc cgacgggatc agtaacccga
    14041 gaacagcgaa gaggcctgac agtaccgaga acgcgacctg cgccaacatc agccagtgcg
    14101 cgcggtctgc cacactcttc gccgtcctga gctcgtcgac accgatctct ttgtcatgcg
    14161 atggcgcacg gagccagaga acccaaaacg tggcacacag catgggaatc aggagcagcg
    14221 cggccgattt ctcccatgga tttcgttcgt tgacttgcaa ttccgcggcg acgaaggtgg
    14281 cggcgatgcc ggcagtcaag gtaaccacaa tcttggcggt gctgatcacg tgccgtaggt
    14341 gctttacctc tcgctcatac ggattcatgc cagccccctc cgtccgcagt tttcgtgagt
    14401 atcgcaagcg cggtgcgaga atgtggccat gcgcagcgac ggccgtggac gcccgaggct
    14461 ggagcagtcg cggcggccgg gcaagtccgc gcgcgaagaa attctggacg cggcggccga
    14521 gctctttacc aacctcggct atgcgagtac ctcgacgcga cggatcgccg acgccgttgg
    14581 cgtgcgccaa gcctcgctgt atcaccactt cgccaccaag gacgacatcc tcgatgcgct
    14641 gctggccggt acggtcgacg aaccgttaga actcgccgcc gagctcctcg acgaagaagg
    14701 cccggcggtg cagcgcctgc atgcgctggt ggtcggcgac gtcacccagc tgtgcgcgag
    14761 ccgatggaac ctcggcgcgc tgtatctact gcccgagttg cggctcgacc ggttcgaaca
    14821 gttccggctg cgccgagccg aattgcgggc ccgctatcgc accctcgccg ccgaggtcat
    14881 cgaggaatgc gacggtcccg tcgacgccga cgacctgccg tttcggctgg tcgaatcggt
    14941 gatcaaccgt cgctccgacg acaccgattg tccagacgag cagccgtggg tcatcgctga
    15001 cggggcgctg cgcacactgg gattcgacgg gggcttcgcc aaacttcaca agctgaccgc
    15061 ggcgcgactg gggtttgccg atacagtcca ggtgtgaccg aataccagac catcacgttc
    15121 gagcagtccg ggcccatcac gcggatcacg ctagaccggc ccgacgcggc aaacggcatg
    15181 aacgacacca tgactcggga actcgccgac gccgccaaac gctgcgactc gcctgccacc
    15241 aaggtggtcg tgatcaccgg atcgggccga ttcttttgcg ctggagggga tctgaagtcg
    15301 ttcgcggcgg cggagaccag ggggccgttc atcaagggcg tcgccgacga cctgcaccgc
    15361 gcgatctcga cgttcgcacg gatggacgcc gtggtgatca cctcggtcaa cggcgtggcg
    15421 gccggcgccg gtttctctct ggccgtgacc ggtgatctgg tgctggcggc cgagtcggcg
    15481 tcgttcacaa tggcctacac gcgagcgggc ctcagccccg acggcagctc gtcgtacttc
    15541 ctgccaaggc tgatcggcat caccaagacc aaggagttga tgctgaccaa ccggactctc
    15601 acggcgccag aggcgtcgca gtggggactg gtcaccgagg tcgtccccga cgcggaactg
    15661 gccgcgcgca ccgacaagct cgctcaccaa atggccgcca ccgcaagggg atccaacggc
    15721 ggcgtcaagg ccctgctgct ctccacattc gccaacgggc tcgaagagca gatggaactc
    15781 gagggccgtc tcatcgccgg tctggccgac tccgtcgacg gccgcgaggg catcgacgcg
    15841 ttcctggcga agcgtaaagc cgagttcgcc tagaaggagt gctcctcggc ggggaaggcc
    15901 ccgctggcca cctcgtcagc gtattgggtt gccgcgctgc gtaattcgct gcccacatca
    15961 ccgaagcgct tgacgaactt ggccgtcttg ccgctggtga acccggccat gtcctgccac
    16021 accagcacct gcgcgtcgca gttcggtcct gcgccgatgc ccaccgtggg gatggtcagc
    16081 ttgccggtga tctgggtggc cagctcggcc ggcaccatct ccagcacgac cgcgatcgca
    16141 ccggcctctt gcacggcgat cgcgtcgtga atggtctgct cggcggcgtc accgcggccc
    16201 tgcacccgga agccgccgag gccgttcacg ctctgcggcg tgaacccgat gtgcgccacc
    16261 actgggatgc cggcctgggt gatcgtcgcg atctgatcgg cgacgcgttc gccgccttcc
    16321 agcttgaccg catgcgcgcc ggtttccttg aggaatcggg tcgcggtggc cagtgcctgc
    16381 tgcgggccgg cctcgtagct gccgaacggc atatccgcga cgacgagcgc gtgcggagca
    16441 ccccgcacca caccacgcac cagcggaatc agctcgtcga tggtcacggg cacggtcgtg
    16501 tcgtagccgt agacgacgtt ggcggcggag tcaccgacga gcaacaccgg aatctcggcg
    16561 tcgtcgaaga tgcgggcggt ggagtagtcg taggccgtca gcatggccca tttgtggcct
    16621 tcggccttcc acttctgcag gtggtgcgtg cgaaccttgc cgcgtggttt aacctcggca
    16681 gcaccgtaaa cggactgctc agacatcgtt gtcccccaat tgggtgggtc gggtcgaatc
    16741 ctcgaggccg ttacggtccc cgggttcgtc tgaccatcta agtctgccac cccgtcacaa
    16801 gaacctgaac ttcaggttaa gtggatttcc tcacactacg atctccggca tgcaacggct
    16861 cagcggactc gacgccagtt tcctctatct cgaaaccgcg aaacagccaa tgcatgtgtg
    16921 ctcgatcctc gagctggaca cctccacgat gcccggcggc tacacgttcg accggctgcg
    16981 cgacgcgctc acgctgcgca tccaggccat gccgcagttc cgcgaaaagc tggccgactc
    17041 ccggttcaac cccgatcatc cggtgtgggt ggacgacaag gactttgacg tcgatcggca
    17101 tctgcaccgc atcggtctgc cttccccggg cggccggcgc gagctcgccg agatctgcgg
    17161 ccacatcgcg tcgctcccac tggaccgaag caggccgctg tgggagacct gggtgatcga
    17221 aaacgtcgca ggcaccgacg cacacgccgg gggccggatt gcggtgatga cgaagatgca
    17281 ccacgccggc atcgacggcg tgacgggcgc caacctgatg tcgcagctgt gcagcaccga
    17341 accggacgcg cccgcgcccg acccggtcga gggcatcggc gatgccagcg gccttgagat
    17401 cgcggtcagc ggtgcggtca agttcgtcac gcggccattg aggctggtca acgtggtgcc
    17461 gaccacgctg acgtcggtcg tcgacaccgt caaacgcgcc cgcagcggcc tggcgatggc
    17521 cgcacccttc gcggcgccga agacagcgtt caacaccaac gtcacgggcc accgcaacat
    17581 cgcgttcgcc caactcgacc tcgaggacat caagacggtg aagaaccact tcggtgtcaa
    17641 ggtgaacgac gtggtgatgg ctctggtgtc gggcgtgctc cgcaagttcc tcgccgaccg
    17701 aggcgaactg cccgacaact cgttggtcgc gatggttccg gtgtcggtgc atgagaagtc
    17761 cgaccggccc ggccgcaatc aggtgtcggg tatgttctcc aaactcgaga cgcacgtcga
    17821 cgaccctgcg gagcggctga agtccatcgc agagtcgaat tcggttgcca agcaacatag
    17881 ttcagcgatc ggtgcgactc tgcttcagga ctggacgcag ttcgccgcgc cggctgtgtt
    17941 cggcgtcgcc atgcgcgtct atgccgcgag ccggctgagc ggcgcacgac cggtgcacaa
    18001 cctggtgatc tccaacgtgc ccggtccaca ggagccgctg tacttcctgg gcaacgagat
    18061 caaggcgatg tacccgctgg gcccgatctt ccacggctcg ggcctcaaca tcacggtgat
    18121 gtcgctgacc ggcaagctcg acgtcggcat cgtgtcgtgc cccgagctgc tgcccgatct
    18181 gtgggacctc gccgatgact tcgaggtggc gctgaacgag ctgctcgacg ccacgagata
    18241 gcgccctcgc ctgcgtggat gcatggtcat ggcagcatgt ggtgccatga acctgcggat
    18301 cggggtcctc gcgaccatcg ctgccctgct gctggccggg tgcagcaaca tggtcgacgg
    18361 gcgcgccgtc atctcggcgc cccggccagg ctctccgatc cagtggttgc cgtgcaaggc
    18421 cgccgcctcg gaccagaacc gcatccccgc gggtgccgag tgcggcatgc tgtcggttcc
    18481 ggtcgactac tccaagcccg acggtgacgt cgcgcagatc gcgatgatcc gcttcaaggc
    18541 caccggccag aaggtcggct cactggtcgt caaccccggc ggtcccggtg aatccggcgt
    18601 ggaggccgcc gcgtcgatgg cgccaacgct gccgcagtcg gtgcgagagc ggttcgatct
    18661 cgtcggcttc gatccccgcg gcgtcgccaa ctccacgccc gcggcgtggt gcaactccga
    18721 cgaggacaac gacaagttgc gggccgatcc cacggtggaa tacacccaag agggtgtcga
    18781 tcacatcgag aaggagaaca aggagttcgt ccagcggtgc gtcgacaaga tgggcaagga
    18841 gttcctggcc aacctcggaa ccgccaacgt ggccaaggat cttgaagcga tccggaccgg
    18901 cctcggcgac gacaaactga cctacctcgg ctactcgtac gggacccgga tcggagccct
    18961 gtacgccgag gcctatcccg acaaggttcg agcgatgatc ctcgacgggg ccgtcgaccc
    19021 gaatgccgac cagatcgagg aggagatccg ccaggccgcc gcgttccaga aggcattcga
    19081 caactacgcc gccgactgcg ccagcagccc ggactgcccg ttgggcactg atccctccaa
    19141 ggcggtggac gtctacaaga gcctcgtcga tccgctggtc aagcacccgg cggagacgaa
    19201 agatccgcgc gggctgagct atagcgacgc gatcgtcggg accatcctgc cgctctattc
    19261 gccgagcctg tggcgccacc tcacccaggc gctgtccgaa atcaaagagg gcagaggcga
    19321 caccatgctg gcgatggccg acctgtacat gggtcgggat gacaaggggc actacaacaa
    19381 ctccaccgat gtccgggtcg cggtcaactg tatggacaag ccgcacatca ccgaccgcgc
    19441 caaggtggtc gacgaggacc gtcgcacccg tgaggtcgcg ccgttcctga gctacggcga
    19501 attcaccggt ctcgcaccgc tggacacgtg cgcgttctgg ccggtgcccg caaccggcga
    19561 tcagcacgag ctcaaggtgg cgggtctgcc gccgatcctg gtggtgtcga cgaccaacga
    19621 tccggccacg ccgtatcagg ccggcgtcga tctggctcag caactcggtg gcacgctgct
    19681 caccttcgac ggcacccagc acaccgttgt cttccaggga aatacgtgtg ttgacgacat
    19741 cgcagcgcgc taccttgtcg acgtgactgt gccgccgccg aatacacgat gctgaccatc
    19801 accacggtcg gcagcgccga tgtgcccgac ctcatgccga tgttgcgggc ctactgtgac
    19861 ttctatcggg ttgatccacc cgacgatcaa ctggcggcac tcgtaactgc gttgatcgac
    19921 gatccgtccg agggacttca gctcatcgcg cgcgacaccg atggaacgcc attgggtttc
    19981 gcgacgatct actggacatg gcagacgctg tacgccgctc gggtcggagt gctcaacgac
    20041 ttgttcgtgg tgcctgcctc ccgcggatcg ggcgcgggcc gcgcgttgat cgaacgctgc
    20101 cgagaactct gccgcgagca tggcgccggg aagctggtgt gggagacggc gccggacaac
    20161 gcgacggcgc aacggcttta cgacggtatc ggcgccgaga agtcgacctg gttgacgtac
    20221 gagctagacg cccgataaca gcaaggtcac cgttgtattc cagccgcaga ccggcggccg
    20281 ttacccatgc gaacatgttg gccatgtggc gggtgggcag gtggagtacg gtgctgctcg
    20341 cgatcttcct gatgtcggcg atcgcgtgcc cggccgcatg ggcgtcgccc gagggtgagt
    20401 cgatccagca gtacggccag ccgccgctgt ggggaagctg cgagcagttc atcggcgacg
    20461 ccacccgcat cccgaccgcg cagtgcggga cggtgtcggt gcccgtcgat tacgccaaac
    20521 ccgaaggcgc ccaagcacag ttggcggtga tccgcattcc cgcatccggc gaccggatcg
    20581 gcgtgctcgt ggtgaacccg ggcggaccgg gtgcgtcggc cgtggacacc gtggccagca
    20641 tgggggcgtc gctggccgac accgacatca tgcgccggtt cgatctggtg ggcgtcgatc
    20701 cgcgcggggt cggccattcc acgccggagc tgcgctgccg caccgacgcc gagttcgatg
    20761 cgttccgtcg tgaaccgatg gccgactaca gcccagaagg tgtcgcccac atcgaagccg
    20821 tctggcgcca ggttgtcgat gcatgcgctc agcgaatggg ttcggagttc ctggccaacg
    20881 tcggaacggc gtcgtcggcg cgcgacatgg acgtcgtccg tgcagcattg ggcgagaacc
    20941 agatcaacta tctcggcttc tcgtacggaa ccgaactggg cgcggcgtat gccgagcagt
    21001 accccgaccg ggtgcgcgcg atggtgctcg acggcgccgt cgaccctagc ctcgacccga
    21061 tcaccaacag cattcgccag atggccggct ttcaaacggc tttcaacgat tacgccgccg
    21121 actgcgcaca gtcggcgggc tgtccgctcg gcaccgaccc cgcgaagttc gtcgaccgct
    21181 ttcacgagtt ggtcgacccg ctggtgaaca agccgggctg gacgtcggat ccgcgtggcc
    21241 tgagctacca ggacgcgata accggcaccg tcaacgcgct ctacacgcgg c
//
LOCUS       DASRJA010000111         6041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3856716,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000111 DASRJA010000000
VERSION     DASRJA010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3856716"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFC01_05270"
     CDS             <1..343
                     /locus_tag="VFC01_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79088.1"
                     /translation="NPYLPPPIYIPRPRPWQPPWQQPPQQEPPQQDPPDDPPTHQNPP
                     PWQWPGSGGGSGGSGSGGSGSGGSGSDDYPGSGSSGGAPQYPGSGSGGSRGGSGGCLL
                     PILCGGGGGRH"
     gene            417..1757
                     /locus_tag="VFC01_05275"
     CDS             417..1757
                     /locus_tag="VFC01_05275"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016890409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZN79089.1"
                     /translation="MRCTVFGTGYLGATHAAGMAGLGHDVIGVDIDAGKVAKLAGGDI
                     PFYEPGLRKMLSDNLAAGRLRFTTDYDEAADFADVHFLGVGTPQKKGEYGADLRHVHA
                     VIDTLVPKLRRTAVIVGKSTVPVGTAAELADRARGLAPEGIDVEVAWNPEFLREGFAV
                     HDTLHPDRIVLGVQRDSIRAEAAIRELYASLLEEGVPFLVTDLQTAELVKVSANAFLA
                     TKISFINAISEVCEAVDADVTLLADALGYDPRIGRRFLNAGLGFGGGCLPKDIRAFMA
                     RAGELGANHALTFLREVDSINMRRRTRMVDLATKACGGSLLGANIAVLGAAFKPESDD
                     VRDSPALNVAGMLQLNGATVNVYDPKAIENSQRLFPTLNYSTSVVEACERADAVLVLT
                     EWREFVELDPDDLADTVRAKVVVDGRNCLDAERWTNAGWRMYCLGKSVNQPTPA"
     gene            complement(1735..2355)
                     /locus_tag="VFC01_05280"
     CDS             complement(1735..2355)
                     /locus_tag="VFC01_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN79090.1"
                     /translation="MARTRIPAAERRARILDAAVETFAEHGFAEAKMQDIAARAGVVP
                     SVLYDHFGSKRELHITVLEQHAEQLRERSLRPIEGASAEELVRVSVANYFKFVEEDPF
                     LFRFLHRDPPADPEIAAVCQEIADRGTEAIAELLRFGAGDAKSVKGITVDEAAWILAR
                     ATQSACAGVATWWYENRDVPRERVVELVFMLLWDGFGGMLKQASAG"
     gene            2429..3700
                     /locus_tag="VFC01_05285"
     CDS             2429..3700
                     /locus_tag="VFC01_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN79091.1"
                     /translation="MALKQRIRWLAMHGTARGVSRLLARRGDPQGRLMTDPTVRSNPV
                     PFTDELRAMGPIVKCRLMYITVDHEIANDLLRSDDFHVFGFGSGLPKPLQRVISWTDP
                     GLLHPVEPPSMLSVEPPDHTRYRKLVSSVFTTRAVAALRDRVQNTADRLVDELAENAG
                     TVDIVERYCSQLPVTVIGDILGVPDEARPRILEFGELGAPSLDMGLSWKQYQQVYRGI
                     EGFNNWLSDHLEQLRRNPGDDLMSQLIQASNNSAEGARLSNEELLATAGLVLAAGFET
                     TVNLLGNGIRMLLDTPEQLETLKARPELWPSAVEEILRLESPVQMTARVAAVDTDIAG
                     TLVLTGEGVLIYVAGANRDPKVFSDPHRFDIERTNAGKHLSFSGGRHFCLGAALARQE
                     GEVGLRTFFERFPDARLAGAGSRRDTRVLRG"
     gene            3802..4155
                     /locus_tag="VFC01_05290"
     CDS             3802..4155
                     /locus_tag="VFC01_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN79092.1"
                     /translation="MSIDHVLAVVPASDVDKSHQWYSSLFGRSADNNPMPTLVEWQVL
                     PGAWVQVFSDPQRAGSGLLNFAVDDLESHLAQVRERGLVPGEIVEANKSVRLSTITDP
                     DGNTIRFIGGFRVEY"
     gene            4170..4922
                     /locus_tag="VFC01_05295"
     CDS             4170..4922
                     /locus_tag="VFC01_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HZN79093.1"
                     /translation="MDFRAALLDQTRDFGELIRSADPSTPVPTCPDWTLRQLFRHVGR
                     GNRWAAQIIADRRTEPLDPRDVREGKPPDDPDAAIDWLNKGAQLIIDSVDRVGSETRV
                     WTFIGARPCGWWIRRRLHEATVHRADAALALGLDYDLSPELAADGISEWIERISVQAR
                     SQPLPLELGRTLHLHATDDGLGPTGEWTITSDREDGVSWSHEHGKGDVALRGPAKELL
                     LAIVRRRTAAEGRVEVLGDTAIWDSWLEHTPF"
     gene            4943..5290
                     /locus_tag="VFC01_05300"
     CDS             4943..5290
                     /locus_tag="VFC01_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN79094.1"
                     /translation="MTTSEIATVLAWHDALTEQDFDTLLQVSSDDIEFGDANGAGQGY
                     EGLQEWASSLAVTAADPGRMYVRGGVVVAEEQDGEAVAFRVVRDHVTSVFRHPDLATA
                     LAATELTEKDLVG"
     gene            5290..>6041
                     /gene="rfbA"
                     /locus_tag="VFC01_05305"
     CDS             5290..>6041
                     /gene="rfbA"
                     /locus_tag="VFC01_05305"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HZN79095.1"
                     /translation="MRGIILAGGSGTRLYPITKGVSKQLLPVFDKPMIFYPLSTLMMA
                     GIRDILVITTPDDAHAFRRLLGDGSDLGINISYAVQDEPNGLAQAFVIGADHIGDGPV
                     ALVLGDNIFYGPGLGTSLSRFQNISGGAIFAYWVANPSAYGVVEFADDGTALSIEEKP
                     ATPKSNYAVPGLYFYDNDVIEIARSLKPSARGEYEITEVNQAYLNQGRLTVEVLARGT
                     AWLDTGTFDSLLDAGDYVRTIERRQGLKISVP"
BASE COUNT         1054 a         1964 c         1979 g         1044 t
ORIGIN      
        1 caacccgtat ctgccgccgc cgatctacat cccgcggccc aggccgtggc aaccgccgtg
       61 gcagcagccg ccgcagcagg agccgccgca gcaagacccg ccggatgatc cgccgacgca
      121 tcagaatccg ccgccgtggc agtggccggg atccggcggg ggctcgggtg ggtccggctc
      181 cggtggctca ggctcgggtg gctcgggttc cgatgactat ccgggttccg gcagttccgg
      241 tggcgccccg cagtacccgg gctccggttc cggtggctct cgcggtggct cgggaggatg
      301 cttactcccg atcctctgcg gcggaggcgg cggtcgccac tagcggtaac cttctgttgg
      361 tctgccgctt ggtgtccctt agcagttagc tcatcgcggc tgcctataga ggcagtatgc
      421 gatgcaccgt atttggcacg ggctatctcg gcgcgacgca cgccgccggg atggccggac
      481 tcgggcacga cgtaatcggc gtcgacatcg acgcaggcaa ggttgccaag ctcgcgggtg
      541 gcgacatccc gttctacgaa cccgggctgc gaaagatgtt gagcgacaac ctcgctgccg
      601 gccggttgcg cttcaccacc gactacgacg aggcggccga cttcgccgac gtgcactttt
      661 tgggcgtcgg aaccccgcag aagaagggtg aatacggcgc cgacctgcgc catgtgcacg
      721 ccgtgatcga caccctggtg ccgaagctgc gccggaccgc ggtgatcgtc ggcaagtcaa
      781 ctgtgccggt cggcacggcc gccgagctgg ccgaccgcgc ccgcgggctt gcccccgagg
      841 ggattgacgt cgaggtggcg tggaatcccg aattcctgcg cgagggcttc gccgtccacg
      901 acaccctgca ccctgatcgc attgtgcttg gtgtgcaacg ggattcgatc cgagccgagg
      961 ctgcgatccg ggagctctac gcctcgctgc tggaagaggg cgtacccttc ctcgtcacag
     1021 accttcagac cgccgaactt gtcaaggtgt ccgcgaacgc ttttctggcc accaagatct
     1081 cgttcatcaa cgcgatctcc gaagtgtgcg aggccgttga cgccgacgtg accctgcttg
     1141 ccgacgcgct ggggtatgac ccgagaatcg gccgtcgctt cctcaacgcc ggcctcggct
     1201 tcggcggcgg atgcctgccc aaggacatcc gcgcattcat ggcccgggca ggcgaactcg
     1261 gtgccaacca cgcactgacg tttctgcgcg aggtcgacag catcaacatg cgcaggcgca
     1321 cccggatggt cgatctggcg accaaggcgt gtggcggatc gctgctcggc gccaacatcg
     1381 cagtgctcgg cgccgcgttc aaacccgagt ccgacgacgt ccgcgactcg cccgccctca
     1441 acgtcgcagg catgctgcaa ctcaacggcg ccacggtcaa cgtctatgac ccgaaggcga
     1501 tcgagaactc gcagcgcctg tttccgacgc tgaactattc gacgtctgtg gtggaggcct
     1561 gcgagcgcgc cgacgccgtg ctggtgctca ccgagtggcg ggagttcgtc gagctcgatc
     1621 ctgacgacct cgccgacacg gtgcgcgcca aagtcgttgt ggacggccgc aattgtctcg
     1681 atgccgaacg gtggaccaat gccgggtggc ggatgtactg ccttggcaag agcgtcaacc
     1741 agccgacgcc tgcttgagca ttccgccgaa gccgtcccac aacaacatga agacgagttc
     1801 gaccacgcgt tcgcgtggca cgtcgcggtt ttcataccac cacgtggcaa ccccggcgca
     1861 cgccgactgt gtggcgcggg caaggatcca ggccgcttcg tccacggtga tgcccttgac
     1921 cgacttggcg tcccctgcgc cgaaccgcag gagttcggcg atggcctcgg tgccacgatc
     1981 ggcaatctcc tggcagaccg cggcgatttc cgggtcggcc ggcggatcgc ggtgcagaaa
     2041 gcgaaacagg aacgggtctt cctcgacgaa tttgaagtag ttcgcgacgc tcactcggac
     2101 caactcctcg gccgaggcgc cctcaatggg tcgcagcgaa cgttccctca actgctcggc
     2161 gtgctgctcg aggaccgtga tgtgaagctc gcgtttggag ccgaaatggt cgtagagaac
     2221 cgatgggacg acgccggcgc gcgcggcgat gtcctgcatc ttcgcctcgg cgaagccatg
     2281 ctcggcgaac gtttcgacgg ccgcgtcgag gatgcgggcc cgccgctctg cagccggtat
     2341 ccgcgtcctt gccataccga atactaattc ggtaggctgc tgacacatta ccgaatcgcg
     2401 attcggtttg gttgggacgg agtgtctgat ggccttgaag caacgcatcc gatggctggc
     2461 aatgcacggc accgcccgcg gagtttcgcg acttctcgcc cgtcggggcg acccccaggg
     2521 ccggctcatg acggacccga cggtgcgaag caatccggtt ccgttcaccg acgaactgcg
     2581 tgcgatgggc ccgatcgtca agtgccgact catgtacatc accgtcgacc acgagatcgc
     2641 caacgacttg ttgcgctctg acgatttcca cgtctttggc ttcggctccg ggctgcccaa
     2701 gccgctgcag cgcgtcatca gctggacgga tccgggactg ctccatccgg tcgagccgcc
     2761 gtcgatgctt tccgtcgagc cgcccgacca cacccgctac cgcaagttgg tgtcgtcggt
     2821 ttttaccacc agagccgttg cggcgctgcg ggatcgcgtg cagaacaccg ccgaccggct
     2881 cgtcgacgag ctcgccgaga atgcggggac tgtcgacatc gtagagcggt attgctcgca
     2941 gcttccggtg acagtgatcg gcgacatcct cggggtgccc gacgaggcgc ggccccgcat
     3001 cctggaattc ggtgaactgg gcgcgcccag cctcgacatg gggctgagct ggaagcaata
     3061 ccaacaggtc taccgtggca tagagggctt caacaattgg ctgtcggatc acctggaaca
     3121 gttgcggcgc aaccctggcg acgacctgat gagccagctc atccaggcgt ccaacaacag
     3181 cgcagaaggt gcacgactga gcaatgagga gctgctggcc accgcgggtc tggtgctggc
     3241 cgccggcttc gagaccacgg ttaacctgct gggcaacgga attcggatgc tgctcgatac
     3301 gcccgagcaa ctcgagacgc tcaaagcgcg gccggagttg tggccgagcg cggtcgagga
     3361 gattttgcgg ctggaatcgc cggtccagat gacggccagg gtggcggccg tcgacaccga
     3421 tatcgccggg acgctggttc tcaccggcga aggcgtcctc atctacgtgg caggcgccaa
     3481 ccgcgatccg aaagtcttct ccgatccgca ccgtttcgac atcgagcgca cgaatgcggg
     3541 caagcatctg tcgttctcgg gtggcaggca cttctgcctc ggcgccgcgc tggcgcgcca
     3601 ggaaggcgag gtcggactgc gcacgttctt tgagcggttc ccggacgcac ggctcgcggg
     3661 tgccggtagc cgccgcgaca cccgggtgct tcggggatga tcgtcgttgc cgatcacgct
     3721 tggcaaggcg cgcgatcccg cgatgactac gcgcgcgtag cgcggtcaat acccctgagg
     3781 accgattaga aggggtaacc cgtgtcgatt gatcatgtgt tggctgtcgt tccggcatcc
     3841 gacgtggaca agagccacca gtggtattcg tcgctgttcg ggcgatcggc ggacaacaac
     3901 ccgatgccca cgttggtcga gtggcaggta ttgccgggag cgtgggtgca ggtcttcagc
     3961 gatccgcagc gggcgggctc gggccttctc aacttcgccg tcgacgacct tgaaagtcat
     4021 cttgctcagg tgcgcgaacg cggcctcgta cccggcgaaa tcgtcgaagc caacaagagc
     4081 gtgcggttgt ccaccatcac cgaccccgac ggcaacacga tcagatttat cggaggtttc
     4141 agagtcgaat attgagctag gttcgtagcg tggatttccg agcggcgctg ctcgaccaga
     4201 cccgggactt cggggaactg atccgcagtg cagacccttc aacgcccgta ccgacctgcc
     4261 ccgactggac gctgcgacag ctgttccgcc atgtcgggcg cggaaaccgt tgggccgcac
     4321 agatcatcgc cgaccggcgg accgaaccgc tggacccgcg cgacgttcgc gagggcaaac
     4381 cgcccgacga ccccgacgcc gcgatcgact ggttgaacaa gggcgcacag ctcatcatcg
     4441 actccgtcga tcgcgtcggt tccgaaacgc gggtgtggac gttcatcggt gcccgcccgt
     4501 gcggctggtg gatccgtcgc cgcttgcacg aggccacggt gcaccgcgcc gacgccgcgc
     4561 tcgcactcgg cctggactac gacctgtcgc cggagctggc cgccgacggg atcagcgaat
     4621 ggatcgagcg catcagcgtc caggcgcgct ctcagccgtt gccgttggag ttggggcgga
     4681 cactgcacct gcacgccacc gacgacggtc ttggtcccac gggtgagtgg acgatcacga
     4741 gcgacaggga ggacggcgtc tcctggtcgc acgaacacgg taagggcgac gtggcgctgc
     4801 gcggcccggc caaggagctg ctgctggcca tcgtgcgcag gcgcaccgca gccgaaggcc
     4861 gcgtcgaagt actcggcgac accgccatct gggatagctg gctggagcac acaccgttct
     4921 aattgggcgt agtttctcga ccatgaccac ttcggagatc gcgaccgtcc tcgcctggca
     4981 tgacgcgctc accgagcagg atttcgacac cctgctgcag gtgtccagcg acgatatcga
     5041 gttcggtgat gcgaacggcg cgggccaggg atatgaaggc ttacaggagt gggcgagctc
     5101 attggccgtc acagctgccg acccaggccg gatgtacgtc cgcggcggtg tggtggtcgc
     5161 cgaggagcag gacggcgagg cagtggcgtt tcgggtggtg cgcgaccacg tcacgtcggt
     5221 attccggcac cccgacctcg ccacggcgct ggccgcgacc gagctgaccg agaaggacct
     5281 cgtcggctga tgcggggcat catcctcgcc ggcggttcgg gcacgcggct ctatccgatc
     5341 accaagggcg tcagcaagca gctgctgccg gtattcgata agccgatgat cttctatccg
     5401 ctgtcgacgc tgatgatggc aggcatccgc gacatcctgg tcatcacgac acccgacgat
     5461 gcgcacgcat tccgccgatt gttaggcgac ggttcggatt tgggcatcaa catcagctac
     5521 gcggtgcagg acgaacccaa cgggctggca caggcatttg tgatcggcgc cgaccacatc
     5581 ggcgacggtc cggtggcatt ggtgttgggc gacaacattt tctacgggcc gggcctgggt
     5641 accagcctga gccgattcca aaacatcagc ggcggagcaa ttttcgccta ctgggtggcc
     5701 aacccttcgg cctacggtgt cgtcgagttc gccgacgacg gcaccgcgct gtccattgag
     5761 gagaagccgg ccacgccgaa gtcgaactac gcggtgcccg gcctgtactt ctacgacaac
     5821 gacgtcatcg agatcgcgcg ctcactgaaa ccctccgcac gcggcgagta cgaaatcacc
     5881 gaggtcaacc aggcctacct gaaccagggc aggctgacgg tcgaggtact ggctcgcggc
     5941 acggcctggc tggacaccgg aacgttcgac tcgctgctgg atgccggcga ctacgtccgc
     6001 accatcgaac gcaggcaggg gctgaagatc agcgtgcccg a
//
LOCUS       DASRJA010000112         3818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2203673,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000112 DASRJA010000000
VERSION     DASRJA010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2203673"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(408..1433)
                     /locus_tag="VFC01_05310"
     CDS             complement(408..1433)
                     /locus_tag="VFC01_05310"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZN79096.1"
                     /translation="MTVIIGVDPHKATHTAVAIDSDERPLARLDVVAERSQTQRLLAW
                     AAPLGKDRRWAVESADGLGKLLAQQLVAAGEDVVDVPPTLAARVRLLGSTKASKNDPN
                     DALATAVAGLGHCGLRAVRVDDHSAVIRMLVDRYDDLTGLRTQAACRLHVALRERIAG
                     GAPKRLSADRAARMLRSVRPVGVAEIERKRLASELLGDVRCLDREIVTARARVAHAVA
                     KSATTLLELHGVGPIVAGFILGHVGDPRRFPSPERFASYNGTAPIEASSGPTKRHRLN
                     PRGNHKLNHAIHMAAVTQVRHDTPGRVYYQRKLTEGKSKKKALRALKRRISDAVWRQL
                     QVDLSRR"
     gene            complement(1713..1871)
                     /locus_tag="VFC01_05315"
     CDS             complement(1713..1871)
                     /locus_tag="VFC01_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79097.1"
                     /translation="MAFFGSMGWIGALPADEAQGLLDEVRSRLIHREHRLLFETDVHW
                     TRLADERG"
     gene            complement(1956..2471)
                     /locus_tag="VFC01_05320"
     CDS             complement(1956..2471)
                     /locus_tag="VFC01_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79098.1"
                     /translation="MSVSARRPAAWVAILALIIVSACQKTVADPVDDELFDIDIVEVG
                     VDYRTDVTRLWLRYTPDSYGASSGAAEWTIALADGTPIVAGPHVFVSHSPTGSTFRAF
                     LCTGPVGSGDGFDGVDTVRLTIDNSCLLPHTGGTLPNAIRVSGRHYVVMGQEIIVTDD
                     TGYTDPVGPYP"
     gene            complement(3042..>3818)
                     /locus_tag="VFC01_05325"
     CDS             complement(3042..>3818)
                     /locus_tag="VFC01_05325"
                     /inference="COORDINATES: protein motif:HMM:NF020664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79099.1"
                     /translation="GTNTFLPSISAEGRYVAFQSDATDLVPGDGNGVYDVFVRDLRAG
                     TTVRASVDVSGGDPDGGSFYPSLNDNGRYVAFHSQATDLISGDGNFDLDVFVRDLHAR
                     TTTRSSVDFLGREQLRQGSLFASISGNGQYVAFQSRASDLVPGDGNGLEDVFVRAVVT
                     PTVDSLTPATVARGSTATLTLTGSGFFPGAQASLQAFGPYGLRVNSVDVVSESELEVL
                     VTVSAAAPTGARDVMVWNPGTGPGPFATGFGFCFGCLTVT"
BASE COUNT          652 a         1250 c         1267 g          649 t
ORIGIN      
        1 ccgccgctgc tccaccgtca accccacccc acgtcatccc tcagcctcct cggatcacgg
       61 accacacagt catcgactca ccgaggagtt gcgcagaccc cttgaacccg cccatctcgg
      121 gtctagctct gcgctgccga attcggctct aagaggatca agggcgccgg cagagccggc
      181 gcccgggctg cctgatcgac ctttggcgct gctcgtcggg ggccgccaga tcgaagcctc
      241 tttgtgtcaa ggggtggttc ttgggcgcgt tgaagcacgc ccctgggggt gggcgtagcg
      301 ttgttgtcgg gcccgggagt gactgatccg aagagccggt gttcagggtt cagccggcca
      361 cgctggattg aagagtcgtc cctggttgtc ctctcgggcc cgctcgatta gcggcggctg
      421 agatcgactt gtagctggcg ccacacggcg tcgctgatgc gacgcttgag cgctcgcagg
      481 gcttttttct tggactttcc ttcggtgagc ttgcgctggt agtagacccg tcccggtgtg
      541 tcgtggcgga cctgggtgac ggcggccatg tggatggcgt ggttcagctt gtggttgccg
      601 cgggggttga gccggtgtcg tttcgttggc ccgctggacg cttcgatggg ggcggtgccg
      661 ttgtaggagg cgaaccgctc tgggctgggg aaccggcggg ggtcaccgac gtgaccgagg
      721 atgaacccag ccacgatcgg gccgacgccg tggagctcga ggagtgtcgt tgcggacttg
      781 gcgacggcgt gagcgacccg ggctcgggcg gtgacgatct cacggtcgag gcatcggacg
      841 tcgcctagta gctcgctggc gagtcgcttg cgttcgatct cggcgacccc gaccggcctg
      901 actgagcgca acatgcgggc cgcacgatcg gccgagagac gcttgggtgc gccgccggca
      961 atgcgttcgc gcaacgccac gtgcagacga catgcggcct gggtgcgcag accggtgagg
     1021 tcgtcgtagc ggtccaccag catccggatc accgcggagt ggtcatcgac acgcacggct
     1081 cgtagcccgc agtgacccag cccggcgact gcggtggcca gggcgtcgtt ggggtcgttc
     1141 ttcgacgcct tggtcgagcc gagcaggcgc acccgagctg ccagcgtcgg cggcacgtca
     1201 acgacgtcct caccggccgc caccagctgc tgggccagca gcttgcccaa tccgtcggcg
     1261 gactcgaccg cccatcggcg gtccttcccc aagggagcgg cccacgccaa cagccgctgt
     1321 gtctgggacc gctcagcgac cacgtcgagg cgggcaagtg gccgctcgtc gctgtcgatt
     1381 gcgacagccg tgtgggtcgc cttgtgcggg tcgacaccga tgatcaccgt catggagttc
     1441 ctcctcgtgt acgcgtggaa gcgtcacgac gggcactcct gatgaagggt tgctgctcgc
     1501 ctctttcgag ccacgccgtg accggacccc gaccggctga cgcttcgttc gggagctaac
     1561 ccgacacgcg agcagcacgc agttcgagag ccagccgacc ggaacccgac gacgctagag
     1621 ccacaacccc acgcccaccc gcagcatcca tcaggcagtt atggcgcgtc tggtatcgac
     1681 cgtctgcgct cacggcggtg acggccaggc tgtcagccgc gctcatcggc gagccgtgtc
     1741 cagtgaacgt cggtctcgaa cagcaggcgg tgctcgcgat ggataagtcg tgacctcacc
     1801 tcatcgagca gaccttgggc ttcgtccgca ggcagcgcgc caatccagcc catcgacccg
     1861 aagaacgcca ccaggtcgtc tcggccaacc gtacatgtat tcgcgaattt agactcgtgc
     1921 agtggttcga cgcgtcgaca catatggcgg tttccctagg ggtacgggcc gacgggatcc
     1981 gtgtagccgg tgtcatccgt cacaatgatt tcctggccca tgacgacgta gtgacggccg
     2041 gaaactcgga tcgcgttcgg cagcgtgccg cccgtgtggg gaaggagaca actgttgtcg
     2101 atggtcaacc tgaccgtgtc gacgccgtcg aagccatcgc cgcttcccac gggcccggtg
     2161 cataggaaag cgcggaacgt cgaaccggtc ggcgaatgcg agacgaacac gtgcggtccg
     2221 gccacgatgg gcgtcccgtc ggccagggcg atggtccact ctgcagcacc cgagctcgcc
     2281 ccataggagt caggcgtgta ccggagccac aacctggtca cgtcggtccg gtagtcgacc
     2341 cccacttcga cgatgtcgat gtcgaacagt tcatcatcga ccgggtcggc gaccgtcttc
     2401 tggcacgcgc tcacaattat gagcgcgagg atcgcgaccc aagccgccgg gcggcgggcg
     2461 gacacactca cgaggtgacg ctatacccgc gcatgccccg tgtctccggt taggtagccg
     2521 cccggcaaag tcagtccgtg gttcctcaga gcaaaggcgt tcggcctcag tgtgcttcgt
     2581 catgccagat ccacgactcg tgcggcggcg tctgcgggta gcccggcggc gcatgccatt
     2641 gcgaacggcg tacagattgc cctgatggtc tggtacgcgg gccgcacagc tcaagtcact
     2701 cactcgctct gtacgaagcg cagctcctgg cagccgacga ggccactttg gccgccgcgc
     2761 agtagcaccg agtttcgatc cgcatcgcac cgagcgcatc cctgagcgtg ccagttcgtg
     2821 cgagattgga cacggaccgg agcgcatcag cggtcaccca cggtcaacga gccatgtcag
     2881 cgcaggtcct ggtcgcgatg cagacgcctc ggcccttccc aagctggtga tacaccacga
     2941 cagaccggca gctctgcgcc accacggcaa gtcctccaac gggggccgca ttcaatcccg
     3001 cacaccctga ccgcgtctcg agtcactggc gccgcgtcgg atcaagtgac ggtgaggcag
     3061 ccgaagcaga acccgaaccc cgtcgcgaac ggcccagggc cagtcccggg attccacacc
     3121 atcacgtcgc gtgctccggt cggtgccgcg gcactcacgg tgaccaagac ttcgagttcg
     3181 gactctgaaa ccacgtcgac cgagttcacg cgaagcccgt atggaccgaa cgcctggagg
     3241 gaagcctgcg caccggggaa gaagccgcta ccggtgagag tgagtgtggc cgtgctgccc
     3301 cgagcgaccg tcgctggtgt gagcgagtcg accgtcggcg tcacgacggc ccgcacgaaa
     3361 acgtcctcaa ggccgttccc gtcgccggga acgagatcac tcgcgcggga ctggaaggcg
     3421 acgtactgtc cgttgccgct gatcgatgcg aagaggctgc cttgcctgag ctgttcccgg
     3481 ccaagaaagt cgacgcttga tcgcgttgtc gtccgggcgt ggagatcgcg cacgaacacg
     3541 tcgaggtcga agttaccgtc gccggaaatc aggtcagtgg cctgggagtg gaaggcgaca
     3601 tagcggccgt tgtcattcag cgacgggtag aaactgccac cgtcgggatc gccgccgctc
     3661 acgtcgacac tggcccgaac ggtggtgccc gcccgcaagt cccggacgaa tacatcgtag
     3721 acgccgttgc cgtcaccggg aacgagatcg gtcgcgtcgg actggaaggc gacgtagcgg
     3781 ccctcggcac tgatcgacgg caagaaggtg ttcgtgcc
//
LOCUS       DASRJA010000113         4591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4138667,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000113 DASRJA010000000
VERSION     DASRJA010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4138667"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3082
                     /locus_tag="VFC01_05330"
     CDS             <1..3082
                     /locus_tag="VFC01_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HZN79100.1"
                     /translation="AGSACNDSYTVKLVVTDGQTCPSNCEKTINVVDTSAPVLSGVPS
                     GGDLGCNPTTLPSCATATATDNCDSSPTVTCVPGAITGTCNKSQTFTYTATDDCGNSA
                     VSTATYTWKQDTTNPVLVGVPAGGDLGCNPTLPTCANVTATDNCDGSLGAATCVPGAI
                     TGAGCLKSQTFVYSAVDACGNSASTSAIYTWKEDTTEPVLAGVPDGGDLGCNPATLPS
                     CNNGVTASDNCDGGLQVQCAPGEIRSKGCSRAQDFVYTATDACGNSASASVRYGWTED
                     TTPPVLVGVPGGGDLGCNPTAPPSCDTGVTATDDCVRELQVTCTPGAITGDCLKSQTF
                     VYSAVDACGNSASTSAIYTWKEDSTGPDLTCPPNKELQCGDPIVPGFTGTGTATDACG
                     TATVDFSDQPIPPNCTGLPGISRTWTATDDCGNTSTCQQTIVFVDTTPPTIQCPGDVT
                     VECNTPYDPSVTGRPTSSDNCGGEILVDFDDGGLTGPEPGCAGAPIPRTWTVTDNCGN
                     SATCVQIISFEDTHAPSIHCPTDIEVGACDNKPTFTATGEDDCDDTPTIVCVPPSGSE
                     FPVGTTEVTCTATDDCGNSTSCGFDVTVTGLPSCDIEGASAICAGTTTELCGPAGATS
                     WSWTGPGGFSAGTQCVTVGAEGTYTLETTDAKGCKSTCTHDLTLNDQPVCGITGPTET
                     CTGESVDLCGPAGNYSYAWSGPGGFNAASQCITVSTGGTYTLVVTDLVTKCSSDGCSH
                     ELTVAPCAVSCPRTPGFWSAQCDQKSGGSTKFNVAQMTQISECVDGKVDIFTWAAGSD
                     FSSFCSAVSNGGVMNQRRQAKRQFAALLANVCTGELGLVASNGDIIKLDPSTPVSCSG
                     LSSTTIGALITEVDGVLLTLEGQPLSSPVVKAMYSQIIACLDAINNAENVGTVACTSA
                     AAENAMYRDYQTPGDGGLTEVVELYRPSPNPFALATRIAYVVRGTGERVDIGVFDLAG
                     RKIRSLASGFQPAGRYEVTWNGVSDDGARVNNGVYFVRSTLAGQHQSMRVIYVK"
     gene            3188..>4591
                     /locus_tag="VFC01_05335"
     CDS             3188..>4591
                     /locus_tag="VFC01_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79101.1"
                     /translation="MSRALWTVVVTAALASLGVQAVQAQCTYTMLASGAAATVTATPT
                     PLQFNQSSAFWTAIAIRSAAGSNWDLGIYSGVGGPPNCVNGSLGSSVKTTGVDFVVGD
                     FNPGHNVAGIHYPLATRVSGAGSATVEWDDGADLLTVNAPSLTVPTSSSDVIQVWDVF
                     LSAGVTYTISFSRTGADAKALLFKSGAGAYWAGRDSRVLELTSNGTYAATTSGYYGLV
                     VVNDDGVTGSYDIAVGRCEIPTDLVSGVAVSTAGLGQRYYRWDQNGTFHLAVGVRGTA
                     NWNIAAFGDRTGSSWPTCFADPLATSSLAAPAVDFIVGNFDTQIADTSYFVRAFMNNN
                     QGTPSAMVEWDGGSTLSDIVLTDPSNPTISRTTGPSDVLECWDIYLNSGQSYQVFFNT
                     NGADLKLFLFAPSLNWGGRSNAILQRSGSSSAASYVAANTGWHGLVVVNDNGQPGSYD
                     LRVYSQNAVGVGDLPPGV"
BASE COUNT          828 a         1534 c         1504 g          725 t
ORIGIN      
        1 ggctggatcg gcctgcaacg actcgtacac cgtgaagctg gtggtgacgg acggtcaaac
       61 ctgcccgagc aactgcgaga agaccatcaa cgtggtcgac acgtctgctc cggtgctgtc
      121 cggtgttccg tccggtggcg atctgggctg caacccgacg acgctgccga gctgtgccac
      181 ggcgacggcg acggacaact gcgacagcag cccgacggtg acttgcgtgc cgggtgccat
      241 caccggcacc tgcaacaaga gccagacctt cacctacacg gcgaccgacg actgcggcaa
      301 cagcgcggtc tcgacggcga cctacacctg gaagcaagac acgaccaacc cggtgctggt
      361 gggcgtaccg gccggtggcg atctgggctg caacccgacg ctgccgacct gcgccaatgt
      421 gacggcgacc gacaactgtg acggcagtct gggagcggcc acctgcgttc ccggcgccat
      481 taccggcgcg ggctgcctca agagccagac tttcgtttac tcggccgtcg acgcgtgcgg
      541 caacagcgcc tcgacgagcg cgatctacac ctggaaggaa gacaccaccg agcccgtgct
      601 ggctggcgtg cctgatggtg gcgacctggg ctgcaacccg gcgacgctgc cgagctgcaa
      661 caacggcgtg accgccagcg acaactgtga cggcggtttg caggtgcagt gcgccccggg
      721 tgagatccgc agcaagggct gctccagggc ccaggacttc gtctacacgg cgaccgacgc
      781 ctgtggcaac agcgcctcgg cgagcgtccg ctacggctgg acggaagaca cgaccccgcc
      841 cgtactggtg ggcgtgcctg gcggtggcga tctggggtgc aacccgacgg cgccgccgag
      901 ctgtgacact ggcgtgacgg cgaccgacga ctgcgtccgc gagttgcagg tgacctgcac
      961 tccgggagcg atcacgggcg attgcctcaa gagccagacc ttcgtttact cggccgtcga
     1021 cgcgtgcggc aacagcgcct cgacgagcgc gatctacacc tggaaggaag acagcaccgg
     1081 cccggatctg acctgtccgc cgaacaagga gctgcagtgc ggtgatccga tcgtccccgg
     1141 cttcacggga acgggaacgg ctaccgatgc atgcggcacc gcgacggttg acttcagcga
     1201 ccagccgata ccgccgaact gcaccggcct gccggggatc agccggacct ggaccgcgac
     1261 cgacgactgc ggcaatacca gcacctgcca gcagaccatc gtgttcgtcg ataccacgcc
     1321 gcccaccatt cagtgcccgg gtgacgtcac ggtcgagtgc aacacgccgt acgacccgag
     1381 tgtcaccgga cgacccacca gcagcgacaa ctgcggtggc gagatcctgg tggacttcga
     1441 cgacggcgga ctgaccggac ctgaacccgg ttgcgccgga gcgccgatcc cccgcacgtg
     1501 gacggtgacc gacaactgcg gcaactcggc gacatgtgtc cagatcatct cgttcgaaga
     1561 cacccatgcg ccgtcgatcc actgtccgac cgacatcgaa gtgggggcct gcgacaacaa
     1621 gccgaccttc accgcgaccg gtgaggacga ttgcgacgac acaccgacga tcgtctgcgt
     1681 cccgccgtcg ggatccgagt tcccagtcgg aaccaccgag gtgacctgca ccgcgaccga
     1741 cgattgcggt aacagcacga gctgcggatt cgacgtcacc gtgactgggt tgccgagctg
     1801 cgacatcgag ggcgcgagtg ccatctgcgc gggcaccacg accgagctgt gcggccccgc
     1861 gggagcgacc agctggagct ggacgggccc gggtggattc tcggctggca cccagtgcgt
     1921 caccgtgggg gcagagggga cgtacaccct cgaaaccacg gatgccaagg gctgcaagag
     1981 cacctgcacc cacgacctga ccctcaacga tcagccggtc tgcggcatca ccgggccgac
     2041 cgaaacctgt acgggtgaga gcgtcgatct gtgcggacct gccggcaact acagctatgc
     2101 ctggtcaggg cccggcggct tcaacgccgc gagccagtgc atcacggtca gcaccggggg
     2161 cacgtacacc ctggtggtga cggatctcgt gaccaagtgc tccagcgacg gctgctcgca
     2221 cgagctcacc gtggctccgt gcgcggtcag ctgcccgcgg acgcccggtt tctggtcggc
     2281 tcagtgtgat cagaagtccg gaggcagcac gaagttcaac gtggcccaga tgacccagat
     2341 ttcggagtgt gtcgacggta aggtcgacat cttcacctgg gcggctgggt cggacttcag
     2401 ctcgttctgt tcggcggtgt ccaacggtgg cgtgatgaac cagcgccgcc aggccaagcg
     2461 tcagttcgcc gccctcctcg ccaacgtctg cacgggtgag ttgggactgg tggcgagcaa
     2521 cggcgacatc atcaagctcg atccgtcgac gccggtttcg tgctcgggcc tcagctcgac
     2581 cacgatcggc gcgctgatca ccgaagtgga tggtgtgctg ctgacgctcg aaggccagcc
     2641 actcagcagt ccggtggtga aggccatgta cagccagatc atcgcctgtc tcgacgccat
     2701 caataacgcc gagaacgtgg gcaccgttgc ctgcacctcg gccgccgcag agaatgccat
     2761 gtaccgtgac taccagactc cgggtgacgg cggcctgacc gaagtggtcg agctgtatcg
     2821 tccgtcgccc aatccgttcg ctctcgcgac ccgcatcgcc tacgtggtgc gtgggaccgg
     2881 cgagcgggtg gacatcgggg tctttgacct cgcgggacgc aagatccgct cgctggccag
     2941 cggcttccag cccgcgggcc ggtacgaagt gacctggaac ggtgtcagcg acgacggggc
     3001 gcgcgtcaac aacggcgtct acttcgtccg cagcaccctc gcgggtcagc accagtcgat
     3061 gagagtcatc tacgtgaagt agcatcacgg aacggtgggc cggggaccat ccggcccacc
     3121 tccggaaaag tcgtggacgg ccccgctcgg ctgcacgcgg cggggccgtt ttactagggg
     3181 gcagtcgatg tcgagagcac tatggacagt ggtcgtcacc gccgcactgg ccagcctggg
     3241 cgtccaggcc gtccaggctc aatgcaccta caccatgctg gcgagcgggg cggcggcaac
     3301 ggtgaccgcg acgccgacgc ctcttcagtt caatcagtcc agcgccttct ggaccgcgat
     3361 cgccatccgc tcggccgcgg gaagcaactg ggacctcggg atctattcag gtgtgggcgg
     3421 accgcccaac tgcgtcaacg gatcgttggg atcctcagtc aagaccaccg gggtcgactt
     3481 cgtcgtgggt gacttcaacc ccgggcacaa tgtggccggc atccactacc cgctcgccac
     3541 ccgcgtcagc ggggcaggct cggcgacggt cgagtgggac gatggcgcgg atctgctgac
     3601 ggtgaacgcc ccctcgttga cagtcccgac cagcagctcg gacgtgatcc aggtatggga
     3661 cgtgttcctg agcgcggggg tcacctacac catcagcttc agccgtaccg gggccgacgc
     3721 caaggccctg ctcttcaaga gcggcgccgg cgcgtactgg gcggggcgtg actcccgggt
     3781 gctggagctc acctccaacg gcacgtacgc cgccacgacg tccggctact acggcctggt
     3841 cgtggtcaac gacgatggcg tcaccggaag ctacgacatt gcggtcgggc gctgtgagat
     3901 tccaaccgac ctggtttcgg gcgtcgcggt cagcaccgcg gggttggggc agcgctacta
     3961 ccgctgggat caaaacggga ccttccatct ggccgtgggc gtgcgcggta ccgccaattg
     4021 gaacatcgcc gcctttggcg accgcaccgg gagttcctgg ccgacgtgct tcgcagaccc
     4081 gctggcgacc tcgagcctgg ccgccccggc ggtggacttc atcgtcggca acttcgacac
     4141 ccagattgca gacacgtctt atttcgtgcg cgccttcatg aacaacaacc agggcacgcc
     4201 ttccgccatg gtggagtggg atggaggctc gactctcagc gacatcgtgc tcaccgatcc
     4261 gtccaatccg accatcagcc ggacgaccgg accctccgat gttctcgaat gctgggacat
     4321 ctatctgaac tccggccagt cgtaccaggt gttcttcaac acgaacgggg ctgacctcaa
     4381 gctgtttctg ttcgccccta gcctcaattg gggaggacgg agcaacgcca tcctgcagcg
     4441 aagcgggagc tcgagcgcgg cgtcttacgt ggccgccaac accgggtggc acgggctggt
     4501 ggtggtcaac gacaacgggc agcccggctc gtacgacctg cgggtctata gccagaatgc
     4561 cgtgggcgtc ggcgacttgc ctcccggcgt c
//
LOCUS       DASRJA010000114         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1040061,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000114 DASRJA010000000
VERSION     DASRJA010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040061"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1126
                     /locus_tag="VFC01_05340"
     CDS             122..1126
                     /locus_tag="VFC01_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HZN79102.1"
                     /translation="MGISKVERLMNLVIALLSTRTFITAERIRETVYGYDDSATDEAF
                     SRMFERDKNELRDLGIPLETGRVSQFDPTEGYRINRDAYALPAVELTADEATAVAVAT
                     QLWESPELITATQGALLKLRAAGVDVDAADSGIAIASTAVLPGLRGSEDVLGILLSAI
                     DSGHAVRFPHRPSRSDPYTTRTVEPWGVVTDRGRWYLVGHDRDRNATRTFRLSRIGAG
                     VTPIGPPGAVKMPDGINLREIVDRAVGEWPSTGQARVWVADGRATALRRHATVVGPLT
                     LGGRAGEEIIIDIGMLDRLGREIASYGPDAVALEPQSLRDDVLARLRAQAGHNDGVTA
                     "
     gene            1123..2097
                     /locus_tag="VFC01_05345"
     CDS             1123..2097
                     /locus_tag="VFC01_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HZN79103.1"
                     /translation="MTAVSTRLVRLLNMVPYFQANPKITYAEAATDLGVSVKQLRDDL
                     NQLWMCGLPGYGPGDLIDFEFSGDTIEVTFSAGIDHPLRLTSPEATGVLVALRTLLDV
                     PGMVDPEAARSAIAKIESAAGTLSHGAEAAVDEPAPAESEAAAAVRAAVRNGRALAIE
                     YYSASHDVLTSRVVDPIRVVLVADHSYLEAWCRSAEGVRLFRFDRIVDAKVLDEPSAP
                     PPPAVQAGPDTSLFDADPSLPSATLLIDRSASWMFDYYPLRVIRELTDGACEASMTYA
                     SDEWMARFVLGFGSAVRVLEPESLAQRVRESATAALDAYEVGADTTGR"
     gene            2128..2379
                     /gene="tatA"
                     /locus_tag="VFC01_05350"
     CDS             2128..2379
                     /gene="tatA"
                     /locus_tag="VFC01_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HZN79104.1"
                     /translation="MGGLQPWHWLIVIAVFVLLFGAKKLPDAARSLGKSMRIFKSEIK
                     EMQSDSKPETPPATPIASERADTPPPAPQAEQPTDQRPA"
BASE COUNT          384 a          909 c          727 g          364 t
ORIGIN      
        1 ctgcccaacg tggggcgatc gccgactggg tctactggca gcacctgagc gtgctgatct
       61 ccgacgcgct ggccgggtcg gccgccaaat aacaccgacc tgaagcctta agctcttgca
      121 agtgggcatt tccaaagtcg agcggttgat gaacctcgtc atcgcgctgc tgtccacccg
      181 cacgttcatc accgccgagc ggatccgcga aaccgtctac ggctacgacg acagcgccac
      241 cgacgaggcg ttctcccgca tgttcgagcg cgacaagaac gagctgcgtg acctgggcat
      301 cccgctcgaa accgggagag tgtcgcagtt cgaccccacc gagggctacc gcatcaaccg
      361 cgacgcctac gccctgcccg ctgttgagct caccgccgat gaggccaccg ccgtcgccgt
      421 cgccacccaa ctctgggaat cgcccgagct catcaccgcc acccagggcg ccctgctcaa
      481 actgcgcgcc gccggcgtcg acgtcgacgc ggcagactcc ggcatcgcga tcgcctcgac
      541 cgccgtgctc cccggcctgc gcggctccga ggacgtgttg ggaatcctgt tgtccgccat
      601 cgactctggc cacgcggtgc gcttccctca ccggccctcc cgcagcgacc cctacaccac
      661 ccgcaccgtc gagccctggg gcgtcgtcac cgaccggggc cgctggtacc tcgtcggcca
      721 cgaccgcgac cgcaacgcca cccggacttt ccggctctcc cgcatcggcg ccggcgtcac
      781 ccccatcggc ccacctggag cggtcaaaat gcccgacggg atcaacttgc gcgagatcgt
      841 cgaccgcgcg gtcggagagt ggcccagcac cggccaggcc cgcgtgtggg ttgccgacgg
      901 ccgtgccacc gcgctgcgcc gccacgccac cgtcgttggc cccctcacgc taggtggccg
      961 ggcgggcgag gagatcatca tcgacatcgg catgctcgac cggctcggcc gcgagatcgc
     1021 cagctacggt ccggacgccg tcgcactcga gccccaatca ctgcgcgacg acgtgctggc
     1081 tcgcctccgc gcccaagccg gccataacga cggggtgacc gcatgaccgc cgtctccacc
     1141 cggctggtcc ggttgctcaa catggtgccg tacttccagg ccaacccgaa gatcacatac
     1201 gcagaagcgg ccaccgacct cggcgtcagc gtcaagcagc tgcgcgacga cctcaaccag
     1261 ctgtggatgt gcggcctgcc cggctacggc cccggcgacc tcatcgactt cgaattctcc
     1321 ggcgacacaa tcgaagtcac gttcagcgcc ggcattgacc acccgctacg gctgacgtcg
     1381 cccgaagcca ccggcgtcct cgtcgcgttg cggaccctcc tcgacgtgcc cggcatggtc
     1441 gaccccgagg ccgcccgcag cgcgatcgcc aagatcgagt ccgccgcggg caccctgagt
     1501 cacggcgccg aagccgccgt cgacgaaccc gcccccgcgg aaagtgaggc tgcggccgcc
     1561 gtccgtgccg ccgtgcgcaa tggccgcgcg ctggcgatcg agtactactc cgcgtcccac
     1621 gacgtgctga ccagtcgcgt cgtcgacccg atccgcgtgg tgctggtcgc cgaccacagc
     1681 tatctggagg cctggtgccg gtcggccgag ggcgtgcggc tgttccgctt cgaccgcatc
     1741 gtcgacgcca aggtgctcga cgagccgtcg gcgccgccgc cgcccgccgt acaggccgga
     1801 ccggacacgt cgctgttcga tgccgacccg tcgctgccgt ccgccacgtt gctcatcgac
     1861 cggtccgcgt cgtggatgtt cgattactac ccgctgcggg tgattcgcga gctgaccgac
     1921 ggtgcctgcg aagcctcgat gacttatgcc tccgacgaat ggatggcccg gttcgtgctt
     1981 ggcttcggct ctgccgtgcg cgtgctcgaa cccgagtcgc ttgcccagcg ggtacgcgag
     2041 tcggccaccg ccgcgctgga cgcctacgag gtcggcgcgg ataccaccgg caggtagact
     2101 cggtccagac gtctggaggt gaccaaattg ggtggtctac aaccctggca ttggctaatc
     2161 gtcattgcgg tattcgtgct gctcttcggc gccaagaagc tcccggacgc agcgcgctcg
     2221 ctcggcaagt cgatgcgaat tttcaagtcc gaaatcaagg aaatgcagtc cgactccaag
     2281 ccggagacgc cacctgctac cccgatcgca tcggaacggg ccgacacccc accaccggct
     2341 ccccaggccg agcagccgac tgaccaacgc ccggcctgac ccac
//
LOCUS       DASRJA010000115         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1315327,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000115 DASRJA010000000
VERSION     DASRJA010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315327"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFC01_05355"
     CDS             <1..276
                     /locus_tag="VFC01_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HZN79105.1"
                     /translation="GMQPKDLDLLVTNQPNRVFLRNWREALELPKERHVDTFDRCGNL
                     FAAGIPVNLDRAISDGTLKKGDVVMMAAFAHAGDFAGAAAIKWGGRN"
     gene            276..1313
                     /locus_tag="VFC01_05360"
     CDS             276..1313
                     /locus_tag="VFC01_05360"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZN79106.1"
                     /translation="MGTLKRELTEAVGALPSALDPLALSLNENPFPPLPAVRSALIES
                     IDAANRYPEFLPERLRSLVAGHVGVGDEQVVIGVGATGVIMQMLRAVTSPGDRMVMTS
                     PTFDGYPIFAQMARLESVTVALDAHGHHDLDAMADAAAHARVVVVCRPHNPTGTIEPA
                     ADIERFLRRVPSDTVVLLDEAYVEFLSPVYRIDAPALVERYPNVVVVRTFSKAYGLAG
                     LRIGYGFCAPDLARRLWTMQLPFGIGITSLVAVAASYDAENQLRQRIRMIASERRFLR
                     MRLRSMGVYSTDGHANFVFLPPWHQPWREVFDGTGLQVRNYADGSVRITVGNRQSTRA
                     VLAAVAPNAMR"
     gene            1356..1859
                     /locus_tag="VFC01_05365"
     CDS             1356..1859
                     /locus_tag="VFC01_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN79107.1"
                     /translation="MAAQRDDRDPIALARANWQRSGWGDVADGMVAVTSVMRAHQILL
                     ARVETALRPYDLSFSRYELLRLLAFSRTGALPITKASDRLQVHVTSVTHAIRRLEADG
                     LVERIPHPTDGRTTLVRITDLGRSTVEDATVTLNNEVFADIGMSDDESRALADSIETL
                     RRNSGDF"
     gene            complement(1897..>2543)
                     /locus_tag="VFC01_05370"
     CDS             complement(1897..>2543)
                     /locus_tag="VFC01_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZN79108.1"
                     /translation="VIEAQEIERRRLAGDIHDGISQRLVTLSYRLDAATRAVKDPEMM
                     SEQLSKARELVDLTLQEARAAIGGLRPPVLDDLGLSGGLASLARSIPQIDMEVDLDDT
                     RLPDHIELALYRIAQECLQNIVKHANAKRARLTFLADSDIARLEIIDDGVGFDTFEHP
                     LGGDEMGGYGLLSMAERAEIVGGRLNIRSRPGSGTAVTATIPLPSPARADGKGP"
BASE COUNT          404 a          836 c          871 g          432 t
ORIGIN      
        1 ggcatgcagc ccaaggatct tgacctgctc gtcacgaacc agcccaaccg ggtgtttctg
       61 cggaactggc gcgaggcgct ggaactgccc aaggagcgcc acgtcgacac attcgaccgc
      121 tgcggcaacc tgttcgccgc aggcatcccg gtcaacctcg accgggccat ctccgacggc
      181 accctgaaga agggcgatgt ggtgatgatg gccgcgttcg cgcacgccgg agacttcgcc
      241 ggggccgccg cgatcaagtg gggcggtcgc aactgatggg cacgctgaag cgagagctga
      301 ccgaggccgt gggtgcgctg cccagcgcgc tggacccgtt ggcgttgtcg ctcaacgaga
      361 atccgtttcc gccgcttcca gcggtgcgct cggcgctgat cgagtccatc gacgcggcca
      421 accgctatcc ggagtttctg cccgagcggc tgcgctcgct ggtcgcgggc cacgtcggtg
      481 tgggcgacga gcaggtggtg atcggggtcg gcgcgaccgg cgtgatcatg cagatgctgc
      541 gtgcggtgac cagtccgggc gaccggatgg tgatgacatc tccaacgttc gacggctatc
      601 cgatcttcgc ccagatggcg cggctggagt cggtgacggt ggcgctggat gcgcacggcc
      661 accacgacct cgacgccatg gccgatgcgg cagcacacgc ccgggtggtc gtggtgtgcc
      721 ggccgcacaa cccgaccggc accatcgagc ccgccgccga catcgagcgc ttcttgcgaa
      781 gggttccctc cgacacggtg gtgctgctgg acgaggccta cgtggagttc ctgtcaccgg
      841 tgtaccggat cgacgcgccc gcactcgtcg agcggtatcc gaatgtcgtg gtggtgcgga
      901 ccttctcgaa ggcctacggg ctggcgggtc tccggatcgg ctatggcttc tgcgcacccg
      961 atctggcccg ccggctgtgg acgatgcagc tgccgttcgg gatcggcatc accagtctgg
     1021 tggcggtggc ggcctcctat gacgcggaaa atcagctgcg gcaacgcatt cggatgatcg
     1081 cctccgagcg acgcttcctg cggatgcggt tgaggtcgat gggcgtgtac agcaccgacg
     1141 gccacgcgaa cttcgtcttc ctgccgccgt ggcaccagcc gtggcgggag gtgttcgacg
     1201 gcaccggatt gcaggtccgc aactacgccg atggcagcgt gcgcatcacc gtcggcaacc
     1261 ggcagtcgac gcgtgcggta ttggcggcgg tggcgccgaa cgcaatgcgc tgagaatctg
     1321 cccggtgttg gccggtgcat gcggtatgaa gaggcgtggc cgcgcaacgg gatgaccgtg
     1381 atccgatcgc gttggcgcgc gccaattggc agcgatccgg ctggggcgac gtcgccgatg
     1441 gcatggtcgc ggtgacatcg gtgatgcggg cccatcagat tctgctggca cgcgtggaga
     1501 cggcgctgcg gccctacgac ctgagcttct cccgctacga attgctgcgg ctgctggcgt
     1561 tcagccgcac cggggcgctg ccgatcacca aggcctcaga ccggctgcag gtgcacgtca
     1621 ccagcgtcac gcacgcgatc cgccggctcg aagcggacgg gttggtggaa cgcatcccac
     1681 atcccaccga cggccgcacc acgctggtgc ggatcaccga tctcggccgc tccacggtcg
     1741 aggacgcgac cgtcacgctc aacaacgagg tgttcgccga catcggaatg tccgacgatg
     1801 agtcgcgggc actagcggac tcgatcgaaa cgttgcgccg caactcggga gacttttagt
     1861 cgcgagcagt cgcaaaagtc ccccgacacg ccgaatttag gggccttttc cgtctgctcg
     1921 cgcgggagag gggagcggga tcgtcgccgt cacagcagtt cccgaacccg ggcgggaccg
     1981 gatattcagc cggccgccga cgatctcggc gcgttcggcc atcgacagca gcccgtagcc
     2041 gcccatctcg tcgccgccca gcggatgctc gaaagtatcg aaacccactc cgtcgtcgat
     2101 gatttcgagc cgggcaatgt cgctgtcggc caagaatgtc agccgcgctc tcttggcatt
     2161 ggcgtgcttg acgatgttct gcagacattc ctgggcgatg cggtacagcg caagctcgat
     2221 gtggtcaggc agccgggtgt cgtcgagatc gacctccatg tcgatctgcg ggatcgaacg
     2281 ggccaagctg gccagcccgc cggacagccc caggtcgtcg agcacgggtg gccgcagtcc
     2341 gccgatcgcc gcgcgagcct cttgcagcgt caggtcgacc agctcgcgcg ccttgctcag
     2401 ctgctcggac atcatctccg gatccttgac ggcccgggtc gccgcgtcga gccggtagga
     2461 cagcgtgacg agccgctgcg agatgccgtc gtggatgtcg ccggccagcc ggcgccgctc
     2521 gatctcctgt gcctcgatca cct
//
LOCUS       DASRJA010000116         3029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1038810,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000116 DASRJA010000000
VERSION     DASRJA010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038810"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..612
                     /locus_tag="VFC01_05375"
     CDS             346..612
                     /locus_tag="VFC01_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HZN79109.1"
                     /translation="MHFQDSDIIPSAWHHVERFANNPIEADHGRLKHRLKAMRGLRTD
                     RTAQVVTACHAFVQNLRRGHYELGHHASRALRVATAFSELARAI"
     gene            complement(718..1686)
                     /locus_tag="VFC01_05380"
     CDS             complement(718..1686)
                     /locus_tag="VFC01_05380"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZN79110.1"
                     /translation="MGDRGRLGVGERAADFVLSGRSGVPARFYAHAGGRPTAVVFDGD
                     GDDRRLADLAGRLADRDDVALCCITSNGTADGPGIPAWSDRNGTVAQAYGVAAGELTA
                     VVLDPNLRVVGVVVGDGLTDRVVELLDTAVHREPTVQVVAQAPVLLVPRALDEGYRDR
                     LIRVWEHDGGTDTGVERVGGDVLDAGFKRRRDHTIDDRDLLRELTTVVGRRVLPEVSK
                     AFAFRATRFEGFKIACYDAATGGFFRPHRDNLTPATAHRVFALTLNLNDDYAGGQLRF
                     PEYGNQLYRPDGGAALVFSCAHLHEVLDVTAGRRFVLLSFLYGEPR"
     gene            1884..2804
                     /locus_tag="VFC01_05385"
     CDS             1884..2804
                     /locus_tag="VFC01_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZN79111.1"
                     /translation="MRGRPIGLVLWLLLLAGVEVAGLVAVWWFFVRSAHGQQLDTVAL
                     AGNRIGQTRVENPVDTVLNTVSVLSLVIATAVIGFIALIRRRVAVAFGVMLLIVGANL
                     TTQLLKEVIDRPQLGIDLPRAAAGNSLPSGHTTTAASVAVALLLVLPAGIRGAGGMLG
                     ALAAAVVGIATLSAGWHRPSDAVAALLIVGVWACAAGLCIVIAQRRHRDVEHGPPNRF
                     AVGALTVVAVVLLAGAALALALTDQALSTPVEQLGRSRLLVAYGGGALGIAGMAGLVV
                     ASVLATAHRIAPQTMPPTVSPGYDATVGKP"
BASE COUNT          481 a         1053 c         1006 g          489 t
ORIGIN      
        1 gctccgctag gagcccaaga atcccgaact tcagtgacat ccctcaccta tacttaccca
       61 gtaacggttt tccagtaacc catactgggt aaccgttgtc aaccctcccc ggcgcggtcc
      121 gcaagccgca cgcctcatgc atggtcgctt cctccggcgc gcaacgcgcc caacggaaca
      181 cggcatcgac ccctagccgt ggcgccggtg cgcgacgaag aaccgctgga tctcatcgaa
      241 gtcgacgcga gcgttggccg atcggtccta tgtggacaat ctgcgggaag ccatcgacca
      301 cgactacacg gtcgtagcgg cgtcccctag cgcacgccgc tacgggtgca ttttcaagac
      361 tcggacatca ttccctcggc gtggcaccac gtcgagcggt tcgccaacaa cccgatcgag
      421 gccgaccacg gccgcctcaa acaccggctc aaagccatgc gcggactacg cactgaccgc
      481 accgcacagg tcgtcaccgc ctgccacgcc ttcgtccaga acctacgccg tggccactac
      541 gaactcggtc accacgcgtc aagggcgtta cgggtcgcca ccgccttcag cgaactcgcc
      601 cgcgcgatct gaccgggccg tgtccggcag acagccatgt ctgccgatca gacaacgcaa
      661 cgtcgccccg acgaccgggt ttcgggatcg tagctctcgc gcagttggtg ttgcatctca
      721 gcgaggttcg ccgtacagga aggacaacag cacgaagcgt cggcccgcgg tcacgtccag
      781 cacctcgtgc agatgggcac atgagaagac cagcgcagct ccgccgtcgg gccggtagag
      841 ctggttgccg tactcgggga agcgaagctg tcccccggcg tagtcgtcgt tgaggttcag
      901 ggtgagtgcg aacacgcggt gcgccgttgc cggggtgagg ttgtcacggt gcggtcggaa
      961 gaagccgccg gtggcggcgt cgtagcaggc aatcttgaac ccttcgaagc gggtggcccg
     1021 gaacgcgaat gctttactga cctcaggcag cacgcggcgg ccgaccaccg tggtcaactc
     1081 ccgcagcaga tcgcggtcgt cgatagtgtg gtcgcgacgc cgcttgaagc ccgcgtcgag
     1141 gacatcgccg ccgacgcgtt ccacgcccgt atcggtcccg ccgtcgtgtt cccacactcg
     1201 gatcagtcgg tcccggtacc cctcatcgag ggcgcggggc accagcagca ccggagcctg
     1261 cgccaccact tgcactgtgg gctcgcggtg cacggcagtg tcgagcagtt cgacgacccg
     1321 atccgtgaga ccgtcgccga ccacaacgcc gaccacccgc agattcggat cgagtaccac
     1381 cgcggtcagt tctccggccg ccaccccgta tgcctgggcg accgtgccgt tgcggtcaga
     1441 ccacgccggt atgcccggac cgtccgctgt cccgttcgac gtgatgcagc acaacgccac
     1501 gtcgtcccgg tccgccagcc gaccggcgag gtcggccaac cgtcggtcat caccgtcgcc
     1561 gtcgaagacc acagcggtcg ggcggccacc cgcatgcgcg tagaaccgcg caggcacacc
     1621 ggaccggccg ctcagaacga agtcggccgc acgctccccg acaccgagcc gcccccgatc
     1681 acccaccccg caccttgcct tccttggatc cgattcctcg tcgagcccga accatactcg
     1741 gtccactcgg ttcacgaacc gaacatcgag ccgacggaca acccgcaggc cagcgcagct
     1801 cacgacggcg cccgagtgta cgacccgtgc ggtgtggcca gcgccacggg taggtcgccg
     1861 gtccgtcggc taggttgctg cgcatgcgtg gacggccgat cggtctggtt ctgtggttgc
     1921 ttctgctggc cggcgtcgag gtggccggct tggtagcggt ctggtggttc ttcgtgcggt
     1981 ccgcacatgg gcagcagttg gacacggtgg cgctggccgg caaccgcatc gggcagaccc
     2041 gggtcgagaa cccggtcgac accgtgttga acaccgtgtc ggtgctgtcc ttggtcatcg
     2101 ccacggcggt catcggcttc atcgcgttga tccgccgccg ggtcgcggtg gcgttcgggg
     2161 tgatgctgct catcgtcggt gcgaacctga cgacgcagct gctcaaggag gtcatcgacc
     2221 gaccgcagct gggcattgat ctgccgcggg cggcggcggg caacagtctg cccagcgggc
     2281 acacgacgac cgcggcgtcg gtcgcggtcg cgttgctgct ggtgctgccg gcggggatac
     2341 gcggggccgg cggcatgctc ggcgcgctgg cggctgccgt cgttggtatc gcgacgctgt
     2401 cggccggttg gcaccgtccc agcgacgcgg tggccgcgct gctcatcgtc ggggtgtggg
     2461 cgtgcgctgc cgggctgtgc atcgtgatag cgcagcgccg gcaccgggac gtcgagcacg
     2521 ggccgccgaa ccgattcgca gtcggtgcgc tcaccgtcgt cgcggtggtg ctactggccg
     2581 gtgcggcact ggcgctggcg ctgaccgacc aggcattgtc cacgccggtc gagcagctgg
     2641 gccgcagccg actgctggtc gcctatggcg gtggcgcgct gggcatcgca ggcatggccg
     2701 gcctcgtcgt ggcctcggtg ctggccaccg cgcaccggat cgcaccacag accatgccac
     2761 ccaccgtcag ccccgggtat gacgcgacag tcgggaagcc ctgaccttgg gcgcagacgg
     2821 tgtcaatgcg acacactggc cgccttcgtc atcccggtcg ggatgcgtgg tgcgtgcggg
     2881 gtgcaactcg agcgctacca cctcgacctg ccgggacgcg acggcgtgat gggttcaatg
     2941 cggtgatcgg aagcgggcgc gatgtggacg ctgatgctgc ggtgacccgc gtacgggcgc
     3001 gagccaagtc gggcgtaacg tctcagctt
//
LOCUS       DASRJA010000117         4406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1037150,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000117 DASRJA010000000
VERSION     DASRJA010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037150"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFC01_05390"
     CDS             <1..204
                     /locus_tag="VFC01_05390"
                     /inference="COORDINATES: protein motif:HMM:NF045154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79112.1"
                     /translation="HLLPLLDGTRDRSALVDALLAIHRENPFPIELDGSQVSDEAEMR
                     DAVTDYIDALPRHLADMKLLRVS"
     gene            complement(197..1447)
                     /locus_tag="VFC01_05395"
     CDS             complement(197..1447)
                     /locus_tag="VFC01_05395"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN79113.1"
                     /translation="MASDDIRPAVSASSPWAPLRIPAFRGLFLAALTSNVGTWMQTVG
                     AQWFLVEQHASATTVALVQTATLSPTLILAIFAGALADRVDRRSLLIALQIYAAVAAA
                     VLTAIAVAGLLNPLSLLSLLFATGCAVAMTTPAWQAIQPELVPREQFPAAASLSGVTI
                     DAARAIGPPIAGVLVAAAGPEAVFAINAVSFAAVVAALMAWKRPPSTPVVTQERLSQS
                     IVTGIRYARDSRVVRRILLRAALFAFPVSALWALLPIAASQHWHLRAIGYGSVLGTVG
                     VGAVLGALVMSRLNRTFSANTLLVLAALIDAAGLLAAAYLPLAVGVVLLLLAGVAWIA
                     TLTTLTVAAQLALPQWVRARGFAMYLVVLIGFQALGAFVWGLVASQFGLAASLTAAAV
                     LLIAAAASQGVLPLLPATSDEPVS"
     gene            1572..2120
                     /locus_tag="VFC01_05400"
     CDS             1572..2120
                     /locus_tag="VFC01_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79114.1"
                     /translation="MERDHEFTVNLTDEDTYTVELLEAVQIERRDDATIGRWPPFNLN
                     AKGDTEDEVYQELLGGLQQQIASGPGSPEFEPFAAYVREHGTRLSDEEAAARELAQLR
                     EMTVRWRVTDDEQYMVRLFQDVEIQRDGDTVTARLRAGRQRPGARCGPAGAEQRHRRG
                     LRRARRTRTPFRRADELGAIEW"
     gene            2125..2505
                     /locus_tag="VFC01_05405"
     CDS             2125..2505
                     /locus_tag="VFC01_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZN79115.1"
                     /translation="MPADVLAQEAKDKQAYLAARGKLAAITPDEITAESSTGVPLLVD
                     FWAEWCGPCRMVTPVLAGLAEQWAGRIVVRKIDVDQFDGIWERFNFRGIPAMIMFKDG
                     KEIHRVIGFGGKAQLVAEVEPHLA"
     gene            2546..2644
                     /locus_tag="VFC01_05410"
                     /pseudo
     CDS             2546..2644
                     /locus_tag="VFC01_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952562.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            2644..3252
                     /locus_tag="VFC01_05415"
     CDS             2644..3252
                     /locus_tag="VFC01_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN79116.1"
                     /translation="MSSRKAEELVKVARSLPGGGHTTLVSDLAEDGAALALLDQAGDI
                     DMLVANAALPASGQLEGFSQDQIGRALRVNLEAPVRLTRELLPRWQERGSGHFVFISS
                     ISGFASTPRASLYAATKFGLRGFALNLREDLRGSGIGVSVITPGAIRDAGMFADSGAP
                     PPPGLGTGTHSRSAMPSCGRSSTTSRRSPWLRFVSGPSPASR"
     gene            complement(3352..3930)
                     /gene="ripD"
                     /locus_tag="VFC01_05420"
     CDS             complement(3352..3930)
                     /gene="ripD"
                     /locus_tag="VFC01_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family peptidoglycan-binding protein
                     RipD"
                     /protein_id="HZN79117.1"
                     /translation="MKRICAVMVGLALLVATPGLATADARNQQAINLVIQRALSQRGV
                     PFAYGGGNIAGPSVGTASVDTPAGTADAGVVADPLSTNPGLNLNPVATPAAEPRVTRV
                     GFDASGLVVYAFAGAGIKMPRSSGEQYKVGQKVLPSQALPGDLIFYGPGGAQSVALFV
                     GNGQMIEATDPAVTVSPVRTNGMAPYLTRIIA"
     gene            4182..>4406
                     /locus_tag="VFC01_05425"
     CDS             4182..>4406
                     /locus_tag="VFC01_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZN79118.1"
                     /translation="MASFHDQDLTGARFDRVSLRDAQMNSVDLSGIQIRGAAFNGCRM
                     RGVELVDVEINGELQNVIVNGVDIVPLVDAE"
BASE COUNT          813 a         1464 c         1429 g          700 t
ORIGIN      
        1 cacctcctcc cactgctcga cggcactcgt gaccgcagtg cactcgtcga cgccctcctc
       61 gctattcacc gtgagaaccc gtttccgatc gagctcgacg gcagtcaggt gtctgacgag
      121 gccgagatgc gcgacgcagt aaccgattac atcgacgcgc tgccgcggca cctcgcggac
      181 atgaagctgc tgcgagtcag ctgaccggct cgtcactcgt cgcgggcagt aacggcagaa
      241 caccctggct cgccgcggca gcgataagaa gaaccgcggc cgccgtgagc gacgccgcaa
      301 gcccgaactg ggaggccact agcccccaca cgaatgcccc gagagcctga aaaccgatca
      361 gcaccaccag gtacatggcg aatcctcggg cccgaaccca ctgcggcaag gcgagctgcg
      421 cagcgacggt aagcgtcgtg agtgtcgcaa tccacgccac accggccagc agcagcagaa
      481 ccacgcccac tgcgagcggc aggtacgccg ccgcaagcaa tcccgcggca tcgatcaacg
      541 ccgcaagcac cagcaacgtg ttggccgaaa acgtcctgtt cagccgggac atcaccagag
      601 caccgagcac cgcccccact cccaccgttc ccagtacgga accgtagccg atcgcgcgca
      661 gatgccaatg ttgcgaggcg gcgatgggca gtaacgccca cagcgccgac accggaaatg
      721 caaacaatgc cgcccgcaac agaattcggc gaacgaccct gctatccctg gcatagcgga
      781 tgccggtgac gatcgactgg ctcagccgct cctgggtgac gaccggcgtc gacggcggcc
      841 gcttccacgc catcagcgcg gcgacgaccg ccgcgaacga caccgcgttg atcgcgaaca
      901 ccgcttctgg acccgccgcg gccaccagca caccggcgat cggcggtccg atcgcccgcg
      961 cggcatcgat cgtgacacca ctgagcgatg ctgccgccgg gaactgctcg cgtggcacca
     1021 gctccggttg aatcgcttgc cacgccggtg tcgtcatcgc gaccgcgcag ccggtcgcga
     1081 acaagagcga cagcagcgac agcggattga gaagaccggc gaccgcaatc gcggtcaata
     1141 ccgccgcggc aaccgcggcg tatatctgca gcgcgatcag caggctccgg cggtcgacgc
     1201 gatcggccag tgcgcctgcg aagatcgcga ggatcagggt cggcgacaaa gtggccgtct
     1261 ggacgagcgc aacagtcgtc gcgctcgcat gttgttccac cagaaaccac tgcgccccaa
     1321 cggtttgcat ccaggtaccg acgttggagg tgagcgcggc gaggaacagc cctcggaacg
     1381 ccgggatccg cagcggcgcc cacggcgacg atgccgacac tgccgggcga atgtcgtcag
     1441 atgccatcgt cgctccctga ggatctatct ctgaccgaaa tgctattggc tgatatggct
     1501 ctttaggact cacttcagca aggcgctgat agcgtttctg tccaaccgct ttgagcgtcg
     1561 gagggggcct catggagcgc gatcacgagt tcaccgtgaa cctaaccgat gaggacacgt
     1621 acacggtcga gctgctcgaa gcggtgcaga tcgaacggcg ggacgacgcc accatcggcc
     1681 gctggccccc cttcaacctg aacgcgaagg gcgacaccga ggacgaggtc tatcaagagt
     1741 tgcttggcgg cctgcagcag cagatcgcca gcggccctgg ttcgccggag ttcgaaccgt
     1801 tcgcggctta tgtgcgggaa cacggcacac ggttgtccga cgaggaagcc gccgcgcgag
     1861 agttggcgca gctgcgcgag atgacggtcc gctggcgtgt cacggacgac gagcagtaca
     1921 tggtccggct gttccaggac gtcgagatac agcgggacgg cgatacggtc accgcgcgcc
     1981 ttcgggctgg aaggcagcgg cccggagctc ggtgcggccc tgcaggcgct gaacagcgcc
     2041 atcggcgagg cttgcggcga gcacgacgca cccggacccc gtttcgacga gctgacgagc
     2101 tgggcgcgat cgaatggtga gccgatgccg gccgacgtcc tggcacagga ggcgaaggat
     2161 aagcaggcct acttggccgc ccgtggcaag ctcgccgcca tcacaccgga cgagatcacc
     2221 gccgagagct cgaccggcgt tccattgctg gtcgacttct gggccgagtg gtgcgggccg
     2281 tgccgcatgg tgacaccggt gctggccggg cttgccgaac agtgggccgg acggatcgtc
     2341 gtccgcaaga tcgacgtcga ccagttcgac ggcatctggg agcggttcaa tttccgcggt
     2401 atcccggcca tgatcatgtt caaggacggc aaggaaatcc accgcgtgat cggcttcggc
     2461 ggcaaggcgc agctggtggc cgaggtcgag ccgcacctcg cttgatgtcg gacgccgcag
     2521 ggatcgccgg aggtaggttc cgggtatcga ccttagcggc aggactgcac tactcaccgg
     2581 cgcgacggga ggcctgggaa gggcgatcgc cacggcgctc gcggcacgcg gagcgcggct
     2641 catttgagct cccgcaaggc cgaggagttg gtgaaggtcg ccagatcgct gccgggcggc
     2701 gggcacacca cgttggtgtc tgacctcgcc gaggacggcg ctgccctcgc attgctcgac
     2761 caggccggcg atatcgatat gctcgtcgcc aacgcagcgt taccggcgtc gggccagctg
     2821 gagggcttta gtcaagacca gatcggcagg gcgcttcgtg tcaacctcga ggcgcccgtt
     2881 cgattgaccc gagagctgct gccgcgctgg caggaacgcg gtagcgggca cttcgtcttc
     2941 atctcgtcga tctcgggctt cgcctccacc ccacgtgcgt ccctctatgc ggccaccaag
     3001 ttcggcctgc gcggcttcgc gctcaaccta cgagaagact tgcgcggcag cggcattggc
     3061 gtttcggtga tcacgcccgg ggcgatccgc gatgccggca tgttcgccga cagtggcgcc
     3121 ccacccccgc ccggcctcgg tacgggaacc cacagcaggt cggcaatgcc gtcgtgcggg
     3181 cgatcgagca cgacaagtcg gagatctccg tggctccgct tcgtcagcgg gccctcgccc
     3241 gcttcgcgat gatggcgccc gagatctccg gtcgcctcgc gggtgccact gcactgaagg
     3301 cggcggacgc gattgccgag ggccagaccg acaagcgata gcggtacggc gtcaggcgat
     3361 gattcgcgtc aggtatggcg ccatgccgtt ggtacgcacc ggtgaaaccg tgacggcggg
     3421 gtcggtggcc tcgatcatct gaccgttgcc gacaaacagc gcgacacttt gggcgcctcc
     3481 ggggccgtag aagatcaagt cgccgggcag cgcctgcgat ggcagcacct tctggccgac
     3541 cttgtactgc tcgccagacg accgcggcat cttgatcccg gcgccggcga atgcgtaaac
     3601 caccagaccg gacgcatcga atccgaccct ggtgactcgc ggttcggcgg ccggggtggc
     3661 gacagggttg aggttgagac cggggttggt cgacagcgga tcggcgacaa cgccggcgtc
     3721 cgcggtgccg gccggagtgt ccaccgaggc agtgccgacg ctgggtcccg caatgttgcc
     3781 gccgccgtac gcaaagggca cgccgcgttg cgacagcgca cgctgaatga ccaaattgat
     3841 cgcttgctgg tttctcgcgt cagcggtcgc caggcccggc gtcgcgacca acaacgccag
     3901 accgaccatc acggcgcaga tgcgtttcat tcgaacttcc ttcatctcgt ggccggtccg
     3961 ccacaactac tcctgtgcgg tttccgggca cccttgaccg cgcgacctat ggaattaaca
     4021 tccgtcacta tattcacttc tgcaacaggt cggtgcgcat cggccggcgt ctcccctggt
     4081 cggcttcgga tctttgctat accgtgaccg cggcgctcac gatttgtgat cgaccgaatc
     4141 ccccacggct ggttgcggtt tcacgcacta ccgtggactt catggcctcg ttccacgatc
     4201 aggacctcac cggagcacga ttcgaccgtg tcagtcttcg cgatgctcag atgaattcgg
     4261 tggatctgag cgggatacag atacgcggtg cggcgttcaa cggttgccgc atgcgtggcg
     4321 tcgaactcgt cgacgtcgag atcaatggcg aactgcagaa cgtcatcgtc aacggcgtcg
     4381 acatcgtgcc acttgtcgac gctgaa
//
LOCUS       DASRJA010000118         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3852226,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000118 DASRJA010000000
VERSION     DASRJA010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3852226"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VFC01_05430"
     CDS             complement(<1..928)
                     /locus_tag="VFC01_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsB family cyclophane-forming radical SAM/SPASM
                     peptide maturase"
                     /protein_id="HZN79119.1"
                     /translation="MACDHCYVYEHADQSWRGRPKFMTTATVRAAAGRIADHAASHHL
                     RRVLVVLHGGEPLLLGVARLRATLEILRDRIEPVTRLDLRMQTNGVLLSEAMCDLLAS
                     FEVKVGVSLDGDREANDRHRRFANGASSYNQVLSALALLRRPEYHHLYAGILCTVDVR
                     NDPNRVYAALAAQSPPRIDFLLPHATWDSPPLRPGPDPTPYSTWLLKIHDLWTSQGRP
                     MSVRLFESLQSTAEGGPSQSEWVGLDPVDLAVIETDGAWEQVDSLKTAFDGAPATGFD
                     VFADPVDTVATWPGIARRQSGIDDLCDTCRSCP"
     gene            complement(1019..1228)
                     /locus_tag="VFC01_05435"
     CDS             complement(1019..1228)
                     /locus_tag="VFC01_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79120.1"
                     /translation="MRDTTNIGQDIVNTTSVSSERIVPSLDAAASTPLDRIPADDVAA
                     VVRRVTKRDSDRPTTVDVAMFGSAI"
     gene            1815..>2287
                     /locus_tag="VFC01_05440"
     CDS             1815..>2287
                     /locus_tag="VFC01_05440"
                     /inference="COORDINATES: protein motif:HMM:NF040095.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79121.1"
                     /translation="MDFQAPPTIPLQRQGELRDVLLEICRVDADGNIDPSRSWQDAVH
                     AVEVLTEIGGGRSGATVLDVNVRMNGQPGFERRVVKVAAVSEIAAEWKALNAVRVRRQ
                     NTLIVPIDAVSSDVQEPSQHWLEGQLAAIVYQHVGAFAAMPHRRVQTLEELVASA"
BASE COUNT          384 a          725 c          778 g          400 t
ORIGIN      
        1 ccgggcagct gcggcaggtg tcgcagaggt cgtcgattcc cgactgtcgt cgcgcgatgc
       61 ccggccaagt ggcaacggtg tcgaccgggt cggcgaagac atcgaagccg gtcgctggtg
      121 cgccgtcgaa cgccgttttc aacgagtcga cctgttccca cgcgccgtcg gtctcgatga
      181 ccgcgagatc gaccggatcg aggccgaccc attcgctttg gctcggacca ccctccgcag
      241 tggactgcag cgactcgaac agccggaccg acatcggccg gccctgactc gtccagagat
      301 cgtggatctt gagaagccag gtcgagtagg gagtcgggtc cggcccgggt cgcagaggcg
      361 gactgtccca cgtggcatgg ggcaaaagga agtcgatgcg gggcggactc tgggccgcga
      421 gcgccgcgta gacccggttc gggtcgttac gaacgtccac agtgcacaag atgccggcgt
      481 agagatggtg gtactcgggc cgtcgcagca gggccagcgc cgagagcact tggttgtagc
      541 tgctggcccc gttggcgaaa cggcgatgtc ggtcgttggc ctcccggtca ccgtccaggg
      601 agacacccac cttcacctcg aaggaagcca gcaggtcgca catcgcctcg gacaggagca
      661 caccgttcgt ctgcatgcgt agatcgagcc gggtcaccgg ctcgatgcgg tcgcgcagga
      721 tctccaacgt cgcccgcagc cgcgccacac ccaggagcag cggttcgccg ccgtgcagca
      781 ccaccagcac ccgccggagg tggtggctcg ccgcgtggtc ggcgatgcgc ccggccgccg
      841 cccgcacggt ggccgtggtc atgaacttgg ggcggccgcg ccagctctgg tcggcgtgct
      901 cgtagacgta acagtggtcg cacgccaaat cacaccgact gtggactttc agtacatact
      961 ggctgatcga cgggtgggca gccggtgacc cacccatcct catggcggac tccgggagct
     1021 agatcgccga accgaacatc gcgacatcca ccgtcgtcgg ccggtccgag tcgcgtttcg
     1081 tcacacgcct gacaacggca gcaacgtcgt cagcgggaat gcggtcgagt ggcgtactgg
     1141 ccgccgcgtc aagcgaggga acgatgcgct cactgctcac cgaagtcgtg ttcacgatgt
     1201 cctgcccaat gtttgtggta tctcgcacga tagttgtgta acgcaagcac cgtatttgcc
     1261 gatgcaaaca ccttattcgc tgtcagccaa tccgcttcgc cgtggcaagg cggcgcgccg
     1321 gtagagagct tatcaatcag ccgtttggtc ggcagttcgg acgtcaaatg catctttgca
     1381 ggtcagaggc ggtctcgtcg gcgtttccgc tgatcccgcc gccgtgttgt gtgagtgatg
     1441 gccgcacgtc gcgccgacgc tttccccggc gcgagtccgg atcttacggt accgccgcgt
     1501 tggtcgcgcg cttcctccag cgtgcggagg tttgactttg cgcacatcat gaaaaagtca
     1561 gatacccggg cccgttgact cgcgtaggtc atctcggcgc gcaagcgccg gtgcgcggag
     1621 cgtgcttgcg gccgtgccag tcaccttgct gtgggtcttg gcgaccgcga tcctgctcag
     1681 ttagcctttc ggctccgagg tcgcctggcc ccgccgggca aaacgcggaa tgcttcggac
     1741 tttcgttcga tggggcgccg accgaagcgc tgcatttaag gcgccgaatg ggtcgtgtag
     1801 gggaggtgcg gaacgtggac ttccaagcgc cgccgacaat tcccctccaa cggcagggcg
     1861 aactgcgtga cgtgctgctc gagatctgcc gcgtcgatgc ggatggcaac atcgacccga
     1921 gtcggtcatg gcaggacgcg gtgcacgcgg ttgaggtgct gaccgagatc ggcggcggtc
     1981 gtagcggggc gaccgtgctg gacgtcaacg ttcggatgaa cggacagccg ggcttcgagc
     2041 gccgggtcgt caaggtagct gccgtctccg agatcgcggc cgaatggaag gcgctgaacg
     2101 cggtacgcgt ccggcggcag aacacgctca tcgtccccat cgacgcggtg agcagcgacg
     2161 tgcaggagcc gtcgcagcat tggctcgagg gccagttggc ggccatcgtg taccagcacg
     2221 tcggtgcctt tgccgccatg ccccaccgcc gggtgcagac actcgaggag ctggtggcga
     2281 gcgcgat
//
LOCUS       DASRJA010000119         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_184665,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000119 DASRJA010000000
VERSION     DASRJA010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184665"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VFC01_05445"
     CDS             complement(<1..539)
                     /locus_tag="VFC01_05445"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HZN79122.1"
                     /translation="MTFLPLTPPGETPVRVMTIAGSDSGGGAGIQADLRTFAMLGVHG
                     LVAVTAVTVQNSVGVKGFHEIPLDVIAGQIEAVASDIGVQAAKTGMLASSAIIDTIAD
                     AWRRQGLAGTVPLVVDPVCASMHGDPLLHPSALDSLRGQLFPLATLVTPNLDEVRLLV
                     DIDVVDFNSQRDAARALHAL"
     gene            662..>2083
                     /locus_tag="VFC01_05450"
     CDS             662..>2083
                     /locus_tag="VFC01_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79123.1"
                     /translation="MAGKRSLRRISDPVVAAAPAAVRIRTRIRVTEAESAALTAIGDF
                     LGSVYRGELARRIRCGVLDRDGRAAWRAERKRAITAVSSSRWAGAITRAAEDQYQLGM
                     RGLAAHVSDLGDAVEVLAERCALRPGEWAPESADEPLSRRRGRQRVRGYRSAAERFTK
                     TRRLAVLRERLAAADQALAAGRPSMVVGGKRLWRSRNHLDAAEMTEQQWRQRWDAVRM
                     FLTADGESGKPGGNETIRVDERSRLRLKVPAALVEQLGTHLQIAAPVRFSHRGAEWAD
                     RVAARRAVRYDISCDPDRGRWYLDASWTITPERAPELDELRAGRVLGVDLNADHLAAC
                     VLDSAGNPIGEPTTIPVAISGLSASRRDGRVRATITALLDHAEQQHCRAVVVENLDFA
                     DARATGRETLGRGARGKTLRRTVAGIPTRRFRVRLTAMAARRGIGVIGVDAAYTSKWG
                     AQHWRTPLQQQTSDPAAVTRHHGA"
BASE COUNT          304 a          666 c          785 g          328 t
ORIGIN      
        1 agtgcgtgca gggcgcgggc ggcatcccgc tggctgttga aatcgacgac gtcgatatcg
       61 accagcaggc ggacctcgtc gaggttgggc gtgaccagcg tggcgagcgg aaacagctga
      121 cctctaagcg aatccagcgc gctcgggtgc agcagcggat cgccgtgcat ggatgcacag
      181 acgggatcga cgaccaacgg caccgtgccg gcaaggccct gtcggcgcca ggcatcggcg
      241 atggtgtcga tgatcgccga cgacgccagc atgccggtct tggcggcctg cacaccgata
      301 tcggaggcca ccgcctcgat ctgacccgcg atcacgtcga ggggaatctc gtgaaagccc
      361 ttgacgccta ccgagttctg cacggtcacc gccgtcacgg cgaccaggcc gtgcacgccg
      421 agcatggcga aggtgcgcag atccgcctgg atgccggcac cgccgcccga gtcggagccg
      481 gcgatcgtca tcacccgcac cggtgtctcg cccggtgggg tcagcggaag gaacgtcata
      541 gcgccaaggg tgccagctgt tggtcggata gtgagtggaa gtgattgatg ctgcgcgacg
      601 gtgggcggtg tggttgcggt ggtcggggct tgatgtcggt ggtgactgcc agcatggcgg
      661 catggctggg aagcggtcgc tgcgtcggat tagtgatccg gtggtggccg ccgcgccggc
      721 ggctgtgcgc atccgcaccc ggattcgggt gaccgaggcg gagtcggcgg cgctgaccgc
      781 gatcggggac tttctgggtt cggtgtatcg cggcgagctg gcccgccgga tccgctgtgg
      841 tgtgctggat cgtgacggcc gggcggcatg gcgtgccgag cgcaagcggg cgatcaccgc
      901 ggtgtcgtcg tcgcggtggg cgggtgcgat cacccgcgcc gccgaggacc aataccagtt
      961 ggggatgcgt ggcctggccg cccatgttag cgatctgggt gacgcggttg aggtgttggc
     1021 tgagcggtgt gcgctgcgcc ccggcgagtg ggcaccggaa tctgccgatg aacctctctc
     1081 gcggcgacgt ggtcgtcagc gcgtgcgggg gtatcgcagc gcggccgagc ggttcaccaa
     1141 gacccggcgg ctggcggtgt tgcgtgagcg cctggccgcc gccgatcagg cgttggcggc
     1201 gggacgcccg tcgatggtgg tgggcgggaa gcggttgtgg cgcagccgca accacctcga
     1261 cgccgccgag atgaccgagc agcagtggcg tcagcggtgg gatgcggtgc ggatgtttct
     1321 gaccgccgat ggggagtcgg gcaagcccgg cgggaacgaa accatccgcg tcgacgagcg
     1381 cagccggctg cgtctgaagg tgcccgctgc cctcgttgag cagctcggca cccacctgca
     1441 gatcgccgcg ccggtgcggt ttagccaccg cggcgccgaa tgggccgacc gggtggccgc
     1501 acgacgggcg gtgcgctacg acatcagctg cgacccggat cggggacgct ggtatttgga
     1561 tgcctcgtgg accatcaccc cggagcgggc gccagaactc gatgagctgc gcgccgggcg
     1621 agtgttgggg gtcgatctca acgccgatca ccttgcggcc tgtgtgctcg acagcgcggg
     1681 caacccgatc ggtgagccca ccaccatccc cgtcgccatc tccgggttgt cggcgtcgcg
     1741 ccgggatggc cgggtgcgcg cgacgatcac cgccctgctc gaccacgccg agcagcagca
     1801 ctgtcgtgcg gtcgtggtcg aaaatctcga cttcgccgat gcccgcgcca ctggacggga
     1861 aaccctgggc cgcggtgcgc gcggcaagac gttgcgccgc accgtcgctg gtatccccac
     1921 ccgtaggttc cgcgtgcggc tcaccgccat ggccgcccgc cgcggtatcg gcgtgatcgg
     1981 ggtggatgcc gcctacacca gcaagtgggg cgcccagcat tggcgtactc ccttgcagca
     2041 gcagacttcc gatccggccg ccgtgacccg ccatcacggc gcg
//
LOCUS       DASRJA010000120        14943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2441723,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000120 DASRJA010000000
VERSION     DASRJA010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14943
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2441723"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VFC01_05455"
     CDS             complement(<1..165)
                     /locus_tag="VFC01_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN79124.1"
                     /translation="MTIALRRSWAKDLDAATLYELLKLRVEVFVVEQACAYPELDGRD
                     LSADTRHFWLE"
     gene            complement(162..1655)
                     /gene="mqo"
                     /locus_tag="VFC01_05460"
     CDS             complement(162..1655)
                     /gene="mqo"
                     /locus_tag="VFC01_05460"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HZN79125.1"
                     /translation="MSDPKAATTDVVLVGAGIMSATLGALLRQAEPNWSITLVERLDG
                     AAAESSDPWNNAGTGHSGLCELNYTPARPDGSIDISKAIQVNEQFQVTRQFWAYAVEN
                     GLLPDVRSFLNPTPHVSFVRGDSRVDYLKRRHEALVANPLFASMEFVDDPDEFARRLP
                     LMAGKRDFSRPVALDWVQDGTDVDFGALSRQLIGYAAQRGMDTLFGHEVRDLGKQSDG
                     RWTLTVVNRRTGAKRRLNARFVFVGAGGGTLPLLQKAGIPEVKGFGGFPIGGRFLRTA
                     NPGLAAAHRAKVYGMLPLGAPHPGASHLDARVINGKPWLLFGPFAGWSPKFLKHGQVT
                     DLPLSVRPNNLASIVNVGLRQAGLVKYLVSQLLLSESDRVQALREFVPAAVDSDWEPA
                     IAGQRVQVIRRGGVLEFGTTVLSAADGTIAGLLGASPGASTAVPAMLDVMERCFPDRY
                     GSWSPKLKEMVPSLGAELSNEPALFEEVWSWGTKVLKLDRPITVAAQ"
     gene            1769..2791
                     /locus_tag="VFC01_05465"
     CDS             1769..2791
                     /locus_tag="VFC01_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN79126.1"
                     /translation="MPGWEPDVLPGYWQQTFDLGPDPDGEGDLAATLVRRGEAEPAAP
                     RAVLLVHGFTDYFFNTEQADHFAARGFAVYALDQHKCGRSWREGQTPHFTTDLARYDA
                     ELERALDLITQDSPEADVLICAHSTGALIVALWLDRLRRRGEITRRRITGLVLNSPWL
                     DLQGPAILRAAPTGAAIGAISRLRKKRVLRPPAEGGYGATLHKEYGGEFDYNLQWKPI
                     GGFPVTYGWLHAIRRGQVRLHRGLDVGVPNLILRSDRSVREVTDPTKVDDIQRGDAVL
                     DVKQIARWAGCIGNRSTIVPIVDAKHDVFLSVPASRQAAYRELDRWLDWYLAETSDTA
                     TQSERG"
     gene            2806..4221
                     /gene="mtr"
                     /locus_tag="VFC01_05470"
     CDS             2806..4221
                     /gene="mtr"
                     /locus_tag="VFC01_05470"
                     /EC_number="1.8.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050627 - mycothione reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HZN79127.1"
                     /translation="MTDFDIAIIGTGSGNSILDERYADKRVAICEQGVFGGTCLNVGC
                     IPTKMFVYAADVAQNAHESAQYGVDAHVDGVRWTDIVSRVFGRIDPIAMGGENYRRSS
                     PNVEVFASHTRFRPPLADGRYRLRTADGDEFTADQVVIAAGSRAVAPDAVTECGVPYH
                     TSDTIMRIADVPEHLIIIGGGFVACEFAHIFSSLGSHVTLLVRGSTLLRGHDDEITMR
                     FTDIASKKWEIRSHHELADARAVGDGVEIRCTDGTTLRGDTLLVATGRVPNGDLLDAE
                     QAGIKVTAGGQVVVDEYQRTSARGIFALGDVSSDYQLKHVANHEARVVKHNLLQDWDD
                     TDALMPSNHRYVPSAVFTDPQIASVGLTENEARAKGFNIKVKVQDFADVAYGWAMEDT
                     TGITKIIVDDDTGLILGAHIMGHQASSLIQPLIQAMAFDLHAQDMARGQYWIHPALPE
                     VVENALLALCGEPPWPPSKRH"
     gene            complement(4218..5321)
                     /locus_tag="VFC01_05475"
     CDS             complement(4218..5321)
                     /locus_tag="VFC01_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HZN79128.1"
                     /translation="MTVVEQPEVTVSTRFDRTDWTTIAAMGSIVLVLHVFGWGVLVFG
                     VAPQHITLGSAGVFGVGLGVTAYLLGVRHAFDADHIAVIDNTTRKLVGEDTRSLSAGF
                     WFSLGHSSVVFGLAFLLAIGVRAIVGPVQDEGSPLLQTLGLVGSLVAGTFLILIGLSN
                     LFAAVGIAKVFRRMRSGDFDEAELERQLENRGFLARLFAKTMRRVSKPWHLYPVGLLM
                     GLGFDTATQVALLVLAAGTAAFTLPWYAILVLPVLFAAGMSLFDALDGILMSRAYGWA
                     FLQPVRKVYYNLTVTVLSVIVALVIGVIVLAGLVTDRLGIDSGPLAAIGSANLEFVGF
                     LIVGLFVAAWFIALAVWRFGQVEKRWAPALDAP"
     gene            complement(5392..6207)
                     /locus_tag="VFC01_05480"
     CDS             complement(5392..6207)
                     /locus_tag="VFC01_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN79129.1"
                     /translation="MTTVEADRALKAKHRALWASGDYPAVAAELIAALGPELVRAIGV
                     KSGDRVLDVAAGSGNAAIPAAAAGGIVTASDLTPELFDAGRQIAAERGVELEWVEADA
                     EAMPFADNSFDAVISCVGVMFAPHHQAAADELVRVVRTGGTIGLINWTPEGLIGNLLA
                     TMKPYAPPPPPGASPPPLWGNEDHVRQLFGERVTGLTARRQTVRMDHCADPLEFREYW
                     KRNYGPTIAAYRFNQDQPERVAALDRDFLAFLTESQRGDGWDAEYLLVTATKR"
     gene            6467..6976
                     /locus_tag="VFC01_05485"
     CDS             6467..6976
                     /locus_tag="VFC01_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010842536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79130.1"
                     /translation="MNTRNKIASMMGGAAVIAAAVGMTAGLTPASAAPSQSPGSMLTI
                     VDQNITGAGYNRVIVQGVYPMQKPDAVGYLNNLTAGCGGMHYIVWGDDGNEQYLFDRN
                     YPGVHEDIDGFVRASDRGLEYQQQFIVPASVLNEDNDGTDEVFVRARFVDGDCGSRVQ
                     NTNVVKGNF"
     gene            complement(7100..9949)
                     /locus_tag="VFC01_05490"
     CDS             complement(7100..9949)
                     /locus_tag="VFC01_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HZN79131.1"
                     /translation="MTVEFRLLGHVEAFLDGRRLDIGHARQRCVLGALLVDVNEPVPA
                     DQLIDRVWADDLPHRARNALAAYISRLRQIFDAAGVQIVRERGSYLLQADALSVDIHR
                     FRHIASRARATADPADAAALFDSALDLWRGEPFASLDTPWINDVRNALEAERVSVTLD
                     RNDAALQAGRHADLLGALVLAAQAHPLDERLAGQLMLAQFRSGRQAQALETYRLMRER
                     LVDELGVDPSPTLRDVYQRILDGDKEAPRPTAPTPDRDAVIALPRRATSFVGRTQELS
                     RLADALHRGPLLTLTGVGGVGKTRLALEVAGRERARFRDGAWLCELAPLDDGAAVGHA
                     IAAALRLQQRQGLDIESTVIEYLATRELLLIVDNCEHVLDTSARLVNRIVQRCPQVTA
                     LCTSREALGIEGETIFAVPPLAVEEATELFADRARAGRPDFDLEREPVGAVAEICRQL
                     DGLPLAIELAASRMRVMSSLDVARRLDTLRLLSGGSRSALPRQQSVTATIDWSYRLLA
                     EAERSLFARLSVFAGGFDLDAAHGVCGEDGADQHDTLDLLTGLVDKSMVTVRSDGGSM
                     RYGVLETLRAYGRERLRDNGVDEEYARRHTAYFAELAQRAAAGMHGPDEGAWVERMLP
                     DYDNLRAAFEHAMTAGGIDVALQLITSLSEFVHLRIGFEASGWAERALRVVDPEHPLF
                     AAVVGFAARGAWNRGDQARARELAALAHGRVPQRGNGRVAYPGDVLADVALYEGDSAA
                     ALAHYEKEMARARRDDDPIRLVWTLFYVAICQAALRTPEAGLAASEEAVHVADVTANP
                     TARSMGRYALGLVLKKSDPDRALALFDEAAELAASVQNFWWHGIALMEAASTRGVHGD
                     PTRAASDFIAVLDHWDRVGDWSQQWLNLRYVTRFLNRVGAEDDAVALHRALVRAGKQS
                     PLSATQSVALGNLPGDALSGADLVLRARSALSRYL"
     gene            complement(9978..12437)
                     /locus_tag="VFC01_05495"
     CDS             complement(9978..12437)
                     /locus_tag="VFC01_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN79132.1"
                     /translation="MATTSLPNRAHVVIIGGGVIGTSIAYHLTKFGFTDVVLLEQGQL
                     SCGTTWHAAGLVGQLRASESATRLVQYSTQLYAELETETGLSAGYKQCGGVTVARTED
                     RMIQLRRTAANAAAFNMECELLTPEAALERYPVMRVDDLVGAIWLPGDGKANPTDLTF
                     ALAKGARMRGAHIIEKTRVTDILTRDGRVRGVRTDVGDIEAEIVVNCAGQWAKQVGAM
                     AGVNVPLHSAEHFYVVSERIDGVHSDLPILRDPDGYTYFKEEVGGLVIGGFEPEAKPW
                     VAPDQIPYPFEFQLLEEDWDHFEILMNSALLRIPALEVTGIKKFYNGPESFTPDNQFI
                     LGEAPEVTNFFVGAGFNSVGIATAGGAGRALAEWIVNGAATSDLTGVDIRRFAPFNGN
                     NRWLRDRVAEVLGIHYEIPWPNREMTTARPFRRSPVHHLLEAAGANFGSKMGWERANF
                     FAPEGAEPAIDYTWGKPNWLDWSAAEQANTRSNVTVFDQTSFSKYLLVGRDAEAALQW
                     LCTADVAAEVGKSVYTGLLNERGTYESDVTVTRTGPEEFLIVSSAATTERDKDHIRKN
                     LPSGTSAALVDVTSAYAVFGVMGPGSRELLSALTSTDLSDEAFPFATSQQISLGYATV
                     RATRITYVGELGWELYVPAEFAVGVYEDLISAGAQFGVGRGGYYTIESLRLEKGYRAF
                     GRELTPSENPVEAGLLFACKLKTDIAFLGREAVEKAKAEGPRKKLVGFRVDAPEAMLW
                     GGELILRDGAVAGQVTSAAWGATTGACVGLAYVRASDNSVINGDWVNSGSYEVNVGGQ
                     LYPVSVSLKPLYDPTNERIRP"
     gene            complement(12449..13369)
                     /locus_tag="VFC01_05500"
     CDS             complement(12449..13369)
                     /locus_tag="VFC01_05500"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HZN79133.1"
                     /translation="MPFPLTDAQLDALLEEIPAVAGRPRQLDELSGGLTNRNVKVTTP
                     DAVYVARCVDTSCNFLAIDRDEEYHNSVAAERAGVGAPVIDYRPDLGILLLGYVNGKT
                     LSNDDFQRPEVVAKVAAGCRTLHSGPRFRSRFDMFERQPQYLKTVLDNGFKIPHDYMD
                     YADTFAQIKRALAADQATVPCNNDLLAGNFIEDGERVWIIDYEYSGNNDPCFELGNIW
                     SECGLSTEQLDELVTAYYGHRLRHKTARAHLQGIVAKYGWTLWGCIQSASSPLEFDFW
                     GWAMERYEAALAEFKSPHLARLLDDVHARD"
     gene            13416..14156
                     /locus_tag="VFC01_05505"
     CDS             13416..14156
                     /locus_tag="VFC01_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZN79134.1"
                     /translation="MPSATEVIETWLSGPQPAVVFDFNGTLSDDEHILFCIFSELFRA
                     HLGWAMTAEDYRAELLGRSDREIVERAVARHGGGSDEKVTELLRLREGVYKQRVADHN
                     PIGASAAQLVKLLVDNEIPVGIVTGAQRDDVLAVLDGSPTGELVSFLIAEEDVSNGKP
                     HPEGFLEAARMLNRRPSDVLVFEDSVPGVQAAVAAGMNCIAVCGSEPSQELSATAPAI
                     VAELSADLVAGPLNRWRLGPGKPIQPAT"
     gene            complement(14107..14895)
                     /locus_tag="VFC01_05510"
     CDS             complement(14107..14895)
                     /locus_tag="VFC01_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN79135.1"
                     /translation="MDDPLLVDRPDLAGAGDLPAHTRIGNWLEQLIVSRTLVPGDKLP
                     SEVDIANALGVSRMTLRQALSAIEAKGLIDRRRGRFGGNFVAAPRFDFDHASLPGFTE
                     QMRRIHVEAGAQVLHATTHRPAADVRQALGLGRGAQVHEILRARSANGEPIVLEEAYL
                     PAAIFPGLLAADLTGSLYAVMREFDASPFSADEELEATQANEQQAEILGVAPGSPLLL
                     ITRTAFTEDGVAVEFSRDYHRSDRTRIRIKSRVESVSPARDASG"
BASE COUNT         2607 a         4991 c         4816 g         2529 t
ORIGIN      
        1 ttccaaccag aagtgccgcg tgtccgccga caggtcgcgg ccgtccaact cgggatatgc
       61 gcaggcctgt tcgaccacga acacctcgac ccgcagcttg agcagttcgt acagcgtggc
      121 ggcgtcgagg tccttggccc agctgcggcg cagcgcgatg gtcattgtgc cgccaccgtg
      181 atgggtctat ccaacttgag cacctttgtg ccccatgacc aaacctcctc gaacagcgcc
      241 ggttcgttcg acagttccgc gcccagcgac gggaccatct ccttgagctt gggcgaccat
      301 gacccatagc ggtcaggaaa gcagcgctcc atgacgtcga gcatcgcagg caccgcagtc
      361 gatgctcccg gcgaggcgcc gagtagcccg gcgatggtgc cgtcggcggc ggacagcacc
      421 gtggttccga attcgagcac accgcctcgg cggattacct gcacccgctg tccggcaata
      481 gcgggctccc aatcggaatc gactgcggcg gggacgaatt cacgcagtgc ctgcacacga
      541 tcggactcgg acagcagtag ttggctgacc aagtacttca ccagacccgc ctgcctgagc
      601 ccgacgttga cgatcgacgc cagattgttg ggcctgaccg acaacggcag atcggtgacc
      661 tggccgtgct tcaagaactt cggcgaccag cccgcgaacg ggccgaacag cagccagggc
      721 ttgccgttga tgacgcgcgc atcgagatgc gacgcccctg gatggggagc gccgagcggc
      781 agcattccgt agaccttggc ccggtgtgcg gcggccagtc ccgggttggc agtgcgcagg
      841 aaccggccgc cgatcgggaa cccgccgaag cccttgacct ccgggatgcc ggccttttga
      901 agcagcggca gggtgccacc gcccgccccg acgaacacga atcgcgcatt cagtcggcgt
      961 ttcgccccgg tgcgccggtt gacgacggtc aatgtccacc ggccgtcgga ttgcttgccc
     1021 aggtcgcgca cctcgtgtcc gaacagcgtg tccatcccgc gctgcgccgc gtagccgatg
     1081 agctgacgcg agagtgcccc gaagtccacg tcggtgccgt cctgcaccca atccagcgcc
     1141 acgggccggg agaagtcacg cttgccggcc atcagcggca accgccgggc gaattcgtcc
     1201 gggtcgtcga cgaattccat cgacgcgaac aacggattgg cgaccagcgc ctcgtggcgg
     1261 cgcttcaagt agtcgacgcg ggagtcgccg cgcacgaagc tgacgtgcgg ggtggggttg
     1321 aggaaactgc gcacgtcggg cagcaacccg ttctccaccg cgtacgccca gaactggcgc
     1381 gtcacctgga actgctcatt gacctgaatc gctttgctga tgtcgatcga gccgtcgggt
     1441 cgagccggcg tgtagttcag ttcgcacagc cccgagtgac cggtgcctgc gttgttccac
     1501 gggtcgctac tctcggcggc ggcgccgtcc aggcgctcga ccagcgtgat cgaccagttc
     1561 ggctccgcct gccgcaacag cgcgcccagc gtggcgctca tgatgccggc gcccaccagc
     1621 acaacgtcgg tcgttgcggc tttggggtca gacaaccgaa tcgtgggccc ttcgtggttc
     1681 tcgacggtta cagctgttca ggctatcccg cggccgtcgg atcctcatcg gcccgggtcg
     1741 ttcgccgctc gcggttaagg tggctttcgt gcctggatgg gagcccgacg tgctgcctgg
     1801 ttactggcag cagacgttcg acctcggccc agatcccgac ggcgaaggcg acctcgccgc
     1861 cacgctggtg cgccgcggcg aagccgaacc ggcggcacct cgcgccgtgc tactggtgca
     1921 cggcttcacc gactacttct tcaacaccga acaggccgac cacttcgccg cccgcggctt
     1981 cgccgtatac gcgttggacc agcacaaatg tggccggtca tggcgcgaag gccagacgcc
     2041 gcacttcacc accgacctgg cccgctacga cgccgaactg gagcgggcgc tggatttgat
     2101 cacccaggac tctccggaag ccgacgtact catctgcgcg cattccaccg gagcgctgat
     2161 cgtcgctctt tggctggacc ggctacggcg ccgcggcgag atcacgcggc gtcgcatcac
     2221 cggcctcgtg ctcaacagcc cgtggctgga tctgcagggg cccgccatcc tgcgcgctgc
     2281 gccgacggga gcggccatcg gcgcgatctc gcgcctccgc aagaagcggg tgctgcggcc
     2341 gccggcggag gggggatacg gcgccaccct gcacaaggag tacggcggcg agttcgacta
     2401 caacctccag tggaagccga tcggcggctt tcccgtcacg tacggctggc tgcacgccat
     2461 ccgccgcggg caggtcaggc tgcaccgcgg gctcgatgtc ggtgtgccca acctgatcct
     2521 gcgctcggac cgcagcgtgc gcgaggtcac cgaccccacc aaggtcgacg acatccagcg
     2581 cggcgacgcg gtgctcgacg tcaagcagat cgcccgctgg gccggctgca tcggaaaccg
     2641 ctccacgatc gtcccgatcg tcgacgccaa gcacgacgta ttcctatcgg tgcccgcgtc
     2701 gcgacaggcg gcgtaccgcg aactggaccg gtggctggat tggtatctcg ccgaaacgtc
     2761 cgatacggca actcaatccg aacgaggatg acgaggttat tggatatgac cgatttcgac
     2821 atcgccatca ttggcacggg ttcgggcaac tcgatcctcg acgaacgcta cgccgacaag
     2881 cgcgtcgcga tctgcgagca aggggtgttc ggcggcacct gcctgaacgt cggatgcata
     2941 ccgaccaaga tgttcgtcta cgccgccgat gtcgcacaaa atgcgcatga gtcagcgcaa
     3001 tacggagtcg acgcccacgt tgacggggtg cggtggacgg acatcgtctc ccgggtgttc
     3061 ggtcgcatcg atccgatcgc gatgggcggt gagaactacc ggcgttcctc cccgaacgtg
     3121 gaggtgttcg ccagccacac ccgattcagg ccgccgcttg ccgacggccg gtaccggctg
     3181 cgcaccgctg acggcgacga gttcaccgcc gaccaggtgg tgatcgccgc cggttcccgg
     3241 gccgtggcac cggatgccgt caccgaatgc ggcgtgccct accacaccag cgacaccatc
     3301 atgcgcatcg ccgacgtgcc ggagcacctg atcatcatcg gcggcggatt cgtggcatgt
     3361 gagttcgccc atatcttttc gtctctcggc agccacgtca cgctattggt ccggggcagc
     3421 acgctgctgc gcggtcacga cgacgagatc accatgcggt tcaccgacat cgccagtaag
     3481 aagtgggaga tccgcagcca tcacgaattg gccgacgcgc gcgccgtcgg cgacggagtc
     3541 gaaatccgtt gcacagacgg cacgacgttg cgcggcgaca cgttgttggt ggcgacgggc
     3601 cgggtcccca acggcgacct gctggacgcc gagcaggcgg gtatcaaggt gaccgcgggc
     3661 ggtcaggtcg tcgtcgacga ataccaacgc acctcggcgc ggggcatttt cgccctcggc
     3721 gacgtgtcgt ccgattatca actcaagcac gtggccaacc acgaggcccg ggtcgtgaag
     3781 cacaacttgc tccaggactg ggacgacacc gacgcgctga tgccgtccaa tcaccgatac
     3841 gtaccgtcgg cggtattcac cgacccgcag atcgccagtg tcggactgac cgaaaacgag
     3901 gcccgggcaa agggtttcaa catcaaggtc aaggttcagg acttcgccga cgtcgcctac
     3961 gggtgggcga tggaagacac caccggcatc acgaagatca tcgtcgacga cgacaccggg
     4021 ttgattctcg gcgcgcacat catgggccat caggcctcgt cgctgattca gccgctgatc
     4081 caggcgatgg ccttcgatct gcatgcgcag gacatggcgc gcggccagta ctggattcac
     4141 cccgcgctgc ccgaagtcgt cgagaacgca ttgttggcgc tatgcggcga gccgccctgg
     4201 ccgccgtcca aacggcatta gggagcatcc agcgctggcg cccagcgctt ttcgacctgt
     4261 ccgaagcgcc acaccgccag cgcgataaac caggccgcga cgaacagccc gacgatcagg
     4321 aagccgacga actcgaggtt cgccgagccg atcgcggcca gcggacccga gtcgatgccg
     4381 aggcggtcgg tcaccaggcc ggccagcacg atgacgccga tgaccagcgc aacgatcacc
     4441 gatagcaccg tcacggtcag gttgtagtac accttgcgca ccggctgcag aaacgcccaa
     4501 ccgtatgccc tagacatcag gatgccgtcc agcgcgtcga acaggctcat gccggccgcg
     4561 aacagcacgg gcagcaccag aatggcgtac cagggcagcg tgaacgccgc cgttcccgcg
     4621 gccagcacca gcagcgccac ctgcgtggcc gtatcgaagc cgaggcccat cagcaggccg
     4681 accgggtaga ggtgccaggg cttgctcacc cggcgcatgg tcttggcgaa gagccgcgcc
     4741 aagaagccgc ggttctcgag ttgccgttcg agttcggcct catcgaagtc gccgctgcgc
     4801 atccggcgga acaccttggc gatgcccacc gcagcgaaca ggttcgacag tccgatcagg
     4861 atgaggaagg tccccgcgac gagggagccc accaggccga gggtctgcaa tagcggcgag
     4921 ccctcgtcct gcaccggccc cacgatcgcg cgcacgccga tcgccaacag gaatgccagc
     4981 ccgaacacca cgctggaatg gcccagtgag aaccagaaac cggcggacag cgagcgggtg
     5041 tcctccccga ccagcttgcg ggtggtgttg tcgatgaccg cgatgtggtc ggcgtcgaac
     5101 gcgtgccgca ccccgagcag gtaagcggtc acgcccaggc cgacaccgaa aacacctgcc
     5161 gatccgagcg tgatgtgctg cggcgccacc ccgaacacca gcacacccca gccgaacacg
     5221 tggagcacca agacgatgga gcccatcgcg gcgatcgtcg tccagtccgt gcggtcgaat
     5281 cgggtcgaaa cggtcacttc gggctgttcg acgacggtca tgggtcgacc ctatgcaggc
     5341 aacccctcat ctgtctagat gaacagatgt tcaggtgtgt cgggttccgt cctatcgctt
     5401 ggtcgcggtg accaacaggt actcggcgtc ccacccgtcg cctcgctgcg actcggtgag
     5461 gaaggccagg aagtcccggt cgagtgctgc gacgcgctcg ggctgatcct ggttgaaccg
     5521 gtaggccgcg atggtcgggc cgtagttgcg cttccagtac tcgcggaact ccaacggatc
     5581 ggcgcaatgg tccatgcgca cggtctggcg tcgagccgtc agaccggtga cccgctcacc
     5641 gaacaattgc cgaacatggt cctcgtttcc ccacagcggc ggagggctgg cacccggcgg
     5701 tggcggtggc gcatacggct tcatggtggc gagcaggttg ccgatgaggc cctccggcgt
     5761 ccagttgatc agtccgatgg tgccgccggt tctgacgact cggaccagct catcggcggc
     5821 ggcctggtgg tgcggcgcga acatcacgcc cacgcacgag ataaccgcgt cgaaactgtt
     5881 gtccgcgaac ggcattgcct ctgcatcggc ctcgacccac tcgagctcca cgccgcgctc
     5941 ggcggcgatc tggcgaccgg cgtcgaacag ttccggcgtc aggtcgctcg cggtgacgat
     6001 tccaccggcc gctgcggccg gaatggccgc gttgcccgag ccggcggcta cgtccagaac
     6061 gcggtccccc gacttcacgc cgatagcgcg gacgagttca ggcccgagcg ccgcaatcag
     6121 ctccgcggca acggcgggat agtcacccga cgcccacagg gcgcggtgct ttgcctttag
     6181 ggcacggtcg gcttcaacgg ttgtcatcgt gttctccttg caggtcggtt cgatgccgtc
     6241 aaggttgctt tggagagttt cggcagacgt ttggcagtcg tgaaatcgcc tgatcagccg
     6301 agctttcggt gtcgtgccca cggctggctc gtgcgcacgc cattgcccgg cggatggtcg
     6361 ttgagctgcc catttcacga atccgtcagg gtcgcagaaa cccgtgcccg cacagtcggt
     6421 ccgtctacca atcgggccgc gaccggcccc aagcacagga gaaggaatga acacacgcaa
     6481 caagatcgca tcaatgatgg gcggggcggc cgtgatcgcc gctgctgtcg ggatgacggc
     6541 cgggctgacg ccggcttcgg cggcgccgtc gcagtcaccc ggctcgatgc tcaccatcgt
     6601 cgaccagaac atcaccgggg cgggctacaa ccgggtgatc gtgcagggcg tgtatccgat
     6661 gcagaagccg gatgccgttg gctacctcaa caacctcacc gcgggttgtg gcgggatgca
     6721 ctacatcgtc tggggcgacg acggtaacga gcagtatctg ttcgaccgca actaccccgg
     6781 agtccatgaa gacatcgacg gcttcgtgag ggcatcggac cgcggcctcg agtaccagca
     6841 gcaattcatc gttccggcga gcgtcctcaa tgaggacaac gacggtaccg acgaggtgtt
     6901 cgtccgagcc cgcttcgtcg acggtgattg cggaagccgg gtgcaaaaca ccaacgtggt
     6961 caagggcaac ttctgacgac ataggcgggc ccggcgtgcg aatgatgttg cgccgggctc
     7021 gtttcgtgtc cggcgagcgc atgtcatcaa cgcccgacag tcactcgaca gttgatccag
     7081 tcggatggcc cgcgcgagct cagagatacc ggctcagggc cgaacgcgcg cggaggacca
     7141 agtccgcacc actcaaagca tctccgggca ggttgccgag ggccacggac tgtgtagcgc
     7201 tcaacggcga ctgtttaccc gcacggacaa gtgcgcggtg gagcgcaacc gcatcatcct
     7261 cggcgccaac gcgattcagg aaccgcgtca catagcgcag gttcagccac tgctggctcc
     7321 agtcgcccac gcggtcccaa tgatcgagca ctgcaatgaa atcgcttgcc gccctggtcg
     7381 gatcgccgtg cacgccacgc gttgacgccg cttccatcaa cgcgatgccg tgccaccaga
     7441 agttctgcac cgaggcagcc aattccgccg cttcgtcgaa tagcgccagc gccctgtccg
     7501 ggtcggattt cttgaggacc agtcccagcg cgtaccgccc catcgaccgt gccgtcgggt
     7561 tggcggtgac gtcggcgacg tgcacggctt cctccgatgc ggcaagaccc gcctccggtg
     7621 tacgcagtgc tgcctggcag atcgcgacgt agaacagcgt ccacaccagc cgaattgggt
     7681 cgtcgtcgcg acgggcccgc gccatctcct tttcgtaatg cgcgagcgcg gcggccgaat
     7741 caccctcgta gagggcgaca tcggcgagca catcgcccgg atacgccacc ctgccattgc
     7801 cgcgttgcgg aacacggccg tgggcaagcg cggccagctc ccgcgccctc gcttgatcac
     7861 cgcggttcca ggcaccccgc gcggcgaagc ccaccacggc ggcgaacaac ggatgctcgg
     7921 gatcgacgac ccggagggcg cgttccgccc aaccggatgc ctcgaagccg atgcgcaagt
     7981 ggacgaactc cgacagggac gtgatcagct gtagcgccac gtcgatgcca ccggcggtca
     8041 tcgcgtgctc gaatgccgcg cgcaggttgt cgtagtcggg cagcatccgc tcgacccagg
     8101 ccccttcgtc ggggccgtgc atgcccgccg cggcgcgctg ggcgagttcg gcgaagtatg
     8161 ctgtgtggcg ccgcgcgtac tcctcgtcaa cgccgttgtc acgcaaccgc tctcggccat
     8221 aggcacgcaa cgtctccagg acgccgtatc gcatgctgcc gccgtcactg cgcacggtca
     8281 ccatcgattt gtcgacgagc ccggtcagta gatcaagtgt gtcgtgctgg tctgctccgt
     8341 cttcgccgca tacgccgtgc gctgcgtcca agtcgaatcc cccggcgaag accgaaagcc
     8401 gtgcgaacaa ggaccgctcg gcctcggcca gcagccgata ggaccagtcg atcgtcgcgg
     8461 tcacactctg ctgcctgggc agcgcgctgc gcgatccacc gctcagcagc cgcagcgtgt
     8521 ccaagcggcg ggccacgtcc aggctgctca tcactcgcat gcgggacgcg gccagctcga
     8581 tcgccaacgg cagcccgtcg agttgccgac agatttctgc caccgctccg acaggctcgc
     8641 gctcgaggtc gaaatccggg cggccggccc tcgcgcggtc agcgaacagc tcggtggcct
     8701 cctcgacggc caacggagga accgcgaata tcgtttcgcc ttcgatgccc aacgcttcgc
     8761 ggctggtgca cagcgccgtt acctgcggac aacgctggac gatccggttg accagccgcg
     8821 cgctggtgtc gagaacatgc tcacagttgt cgacgatcag cagcagttcg cgtgtcgcca
     8881 agtattcgat caccgtcgac tcgatgtcga gcccctggcg ctgttgtagt cgcagggctg
     8941 ccgcaatcgc atggcccacc gccgcaccat cgtcgagtgg cgcaagctcg cacagccatg
     9001 cgccgtcgcg gaagcgcgcc ctttcgcggc ccgccacttc gagcgcgagc cgggtcttgc
     9061 cgacgccgcc cactccagtg agcgtcagca gtgggccccg atgcagagca tcggcgaggc
     9121 gcgaaagttc ttgcgtacgc ccgacgaagc tcgtcgcgcg ccgcggcaag gcaatgacgg
     9181 cgtcgcgatc aggtgtgggc gccgtagggc ggggagcctc cttgtcgccg tcgaggatcc
     9241 gctggtacac gtctcgcagc gtgggactgg gatctacgcc cagctcgtcg acgagtcgct
     9301 cacgcatcag ccgataggtt tccagcgcct gggcctgcct gccgctgcgg aactgggcga
     9361 gcatcagctg cccggccagg cgttcatcca gcgggtgggc ttgggccgcc aatacgagcg
     9421 caccgagcag gtcggcatgc cgtcctgcct gcaatgcggc gtcattgcgg tccagagtga
     9481 ccgagacgcg ttcggcttca agcgcattgc gtacgtcgtt gatccacggc gtgtcaaggg
     9541 atgcgaacgg ctcgccgcgc cacaagtcga gcgcgctgtc gaagagggcc gcagcgtcgg
     9601 ccggatcggc ggtcgctctc gctctggatg cgatgtgccg gaagcgatgg atatccaccg
     9661 acagcgcatc ggcttgcagc aagtaggagc cgcgctcacg gacgatctgc acaccggcgg
     9721 cgtcgaatat ttgtcgcagt cgcgatatgt atgccgcgag cgcgttgcgg gcccggtgtg
     9781 gaaggtcgtc cgcccacacc cgatcgatca attggtcggc gggcacgggc tcgttgacgt
     9841 cgacgagcag cgccccgagc acgcaccgtt ggcgcgcgtg cccgatgtcg aggcggcgcc
     9901 cgtccaggaa cgcctcgacg tggccgagca gccggaactc caccgtcacg tcatacagcc
     9961 tctatcaggc ggacgagcta cgggcgaatt ctctcgttcg tcgggtcgta gagcggcttc
    10021 agcgataccg agaccggata caactggccg ccgacgttca cctcataact gcctgagttg
    10081 acccagtcgc cattgatgac tgagttgtcc gacgcacgca cgtacgccag cccgacgcac
    10141 gcacccgtcg tcgcacccca tgccgcggac gtcacctgtc cggccactgc accgtcacgc
    10201 agaatcaact cacctcccca cagcatcgcc tcgggtgcat cgaccctgaa gccgacgagt
    10261 ttcttgcgtg gtccctcggc tttggccttc tcgaccgcct cgcggccgag gaacgcgata
    10321 tcggttttca gcttgcacgc gaagagcaat ccggcttcga cggggttttc actcggggtc
    10381 agctcccggc cgaatgcgcg gtagcccttt tccaggcgca gcgactcgat cgtgtagtag
    10441 ccgccgcggc cgacaccgaa ctgggctcca gccgagatca ggtcttcgta gacaccgaca
    10501 gcgaactcag cgggaacata gagctcccaa cccaattcgc cgacatacgt gatcctggtc
    10561 gcgcgcacgg tggcgtatcc cagtgaaatc tgctggctgg tggcgaacgg gaacgcctca
    10621 tcggacaggt cggtcgacgt cagcgcggac aacaactccc gagacccggg tcccatcacc
    10681 ccgaacaccg cgtacgcaga cgtcacatcg acgagtgcgg cgctcgttcc ggagggcagg
    10741 ttcttgcgga tgtggtcctt gtcgcgttcg gtggtggccg cgctgctgac gatcaggaat
    10801 tcctcggggc cggtgcgggt caccgtcacg tctgactcgt acgtgccgcg ttcgttgagc
    10861 agaccggtgt acaccgactt gccgacctcg gcggccacgt cggcggtgca cagccattgc
    10921 agcgccgcct cggcgtcgcg gccgaccaac aggtatttcg agaacgaggt ttggtcgaac
    10981 acagtgacat tcgaccgcgt gttggcctgc tcggccgccg accagtcaag ccagttgggc
    11041 ttgccccagg tgtagtcgat cgccggctca gccccctcgg gcgcgaagaa gttggcccgc
    11101 tcccagccca tcttgctgcc gaagttggcg cccgcggcct ccagcaggtg gtgcaccggc
    11161 gagcgccgaa acggccgggc ggtcgtcatt tcccggttgg gccaagggat ttcgtagtgg
    11221 atgccgagca cttccgctac ccggtcgcgc agccagcggt tgttgccgtt gaacggtgcg
    11281 aaccggcgga tgtcgacgcc cgtcaggtcg ctggtcgccg ctccgttgac gatccattcg
    11341 gcaagcgcac gtccggcacc acctgctgtg gcgataccca ccgagttgaa tccagcaccg
    11401 acgaaaaagt tggtgacctc cggagcctcg ccgaggatga actgattatc cggagtgaag
    11461 ctctccggac cgttgtagaa cttcttgatg cccgtgacct cgagcgcggg gatgcgcagc
    11521 agcgcgctgt tcatcaggat ctcgaagtga tcccaatcct cttccaacag ttggaattcg
    11581 aacggatacg gaatctgatc gggcgccacc cacggtttgg cttcgggctc gaagcctccg
    11641 atgaccaggc cgccgacctc ctccttgaag taggtgtagc cgtcggggtc acgcaggatg
    11701 ggcaggtccg aatgcacccc gtcgatccgc tcgctgacga cgtagaaatg ttcggctgag
    11761 tgcagtggga cgttgacgcc ggccatcgca cctacctgct tcgcccattg gccggcgcag
    11821 ttgacgacta tctcggcttc gatgtctccg acgtccgtgc gtacgcctct gacccgtccg
    11881 tcgcgggtga gaatgtctgt caccctagtc ttttcgatga tgtgcgcgcc tcgcatcctg
    11941 gcccccttgg cgagcgcgaa tgtcaggtcg gtggggttgg ccttgccgtc gccgggcagc
    12001 cagatcgcac cgaccagatc gtcgacacgc atcaccgggt aacgctcgag cgctgcttcg
    12061 ggggtgagca gttcgcattc catgttgaac gcggcggcgt tggcggcggt gcggcgcagt
    12121 tgaatcatcc gatcctcggt gcgcgcgacg gtcactcccc cgcactgctt gtagccggcc
    12181 gacagaccgg tctcggtctc gagctcggcg tacagctgcg tcgagtactg caccaaccgg
    12241 gttgcgctct ccgaggcccg tagctgaccc accaggccgg ccgcgtgcca cgttgtgccg
    12301 caggacagtt ggccctgttc gagcagcacg acgtcggtga acccgaactt ggtcaggtgg
    12361 taggcgatgc tcgtgccgat gacgccgccg ccgatgatca caacgtgggc gcggttggga
    12421 agcgatgtgg tggccacagt gtccttatct agtcgcgggc gtgcacgtcg tcgaggagtc
    12481 gggccaagtg cgggctcttg aactcggcga gcgcggcctc gtaccgctcc atcgcccagc
    12541 cccagaagtc gaattcgagt gggctggagg cgctttgaat gcagccccac agcgtccagc
    12601 cgtatttggc gacgatgccc tgcaggtgag ccctggcggt cttgtgacgc agccggtggc
    12661 cgtaataagc ggtgacgagc tcatcgagct gctcggtgga taggccgcat tcgctccaga
    12721 tgttgccgag ctcgaaacat gggtcgttgt tgcccgagta ctcgtagtcg atgatccaca
    12781 cccgctcgcc gtcctcgatg aagttgccgg ccagcagatc gttgttgcac gggacggtgg
    12841 cctgatcggc agccagcgct ctcttgatct gggcgaatgt gtcggcgtaa tccatgtagt
    12901 cgtgcggaat cttgaagccg ttgtccagga cggttttcag gtattgaggc tggcgttcga
    12961 acatgtcgaa ccggctgcga aaccgcggcc cggagtgcag tgtgcggcat cctgcggcga
    13021 cctttgccac cacctccgga cgctgaaagt cgtcattgga cagcgtcttt ccgttcacgt
    13081 agcccaacag caggatgcca aggtccgggc gatagtcgat caccggcgcg ccgacgcccg
    13141 ctcgctcggc agcgacggaa ttgtggtact cctcatcgcg gtcgatggcc aggaaattgc
    13201 aggaggtgtc gacgcaccgg gcgacgtaga ccgcgtccgg ggtggtgacc ttgacgttgc
    13261 gattcgtcag cccgcccgac agttcgtcca attgccgcgg ccgccctgcc accgccggaa
    13321 tctcttccag cagcgcatcg agctgcgcgt cggtcaacgg aaacggcatc ggttcactgt
    13381 aaccctcacc ccgttaaagg tctagactat tggccatgcc gtccgcgacc gaggtcatcg
    13441 aaacgtggtt gagcggcccg cagcccgccg tcgtgttcga cttcaacggc accctctccg
    13501 atgacgaaca cattttgttc tgcattttca gcgagctctt ccgcgcccac ctcggctggg
    13561 cgatgaccgc cgaggactat cgcgccgagt tgctggggcg tagcgaccgc gagatcgtcg
    13621 agcgcgcggt ggcgcgtcat ggcggcggca gcgacgagaa ggtgaccgag ctgctgcgac
    13681 tgcgtgaggg cgtctacaag caacgggtcg ccgaccacaa cccgatcggg gcctctgccg
    13741 cacagttggt gaaactgttg gtggacaacg agattcccgt tggcatcgtt accggggcgc
    13801 agcgcgacga cgtgctcgcg gtgctggacg gcagccctac gggtgaattg gtcagcttcc
    13861 tgatcgccga ggaagacgtc agcaacggta aaccgcatcc ggagggcttt ctcgaagcgg
    13921 cgcggatgtt gaaccgccga cccagcgatg tattggtttt cgaggattcg gtgcccggcg
    13981 tacaggccgc ggtggccgcg ggcatgaatt gcatcgccgt ctgcggtagc gagccaagcc
    14041 aagagctgag cgccactgct cccgcgatcg tggcggaatt gtcggccgat ctcgtcgcag
    14101 gcccgctcaa ccgctggcgt ctcgggccgg ggaaaccgat tcaacccgcg acttgatgcg
    14161 gatccgagtg cggtcggagc ggtggtagtc gcgcgagaac tcgaccgcga caccgtcctc
    14221 ggtgaaggcc gtccgggtga tcaacaataa cgggcttccc ggtgcaacgc cgaggatttc
    14281 ggcctgctgc tcgttggcct gggtcgcctc gagttcttcg tctgccgaga acggggacgc
    14341 gtcgaactcg cgcatcaccg catacagcga gccggtcaga tcagcggcca gcaggccggg
    14401 aaagatcgcg gcgggcagat acgcctcttc gagcacgatc ggctcgccgt tcgccgaccg
    14461 ggcgcgcaag atctcatgga cctgcgcacc gcggcccagt cctagggcct gccgaacgtc
    14521 ggctgccgga cggtgcgtcg tcgcatgcag cacctgcgcg ccagcctcca cgtggattcg
    14581 acgcatctgc tcggtgaacc cgggcaggct ggcgtggtcg aagtcgaatc gcggtgcggc
    14641 gacgaagttg ccgccgaagc ggcccctcct ccggtcgatg aggcccttgg cttcgatggc
    14701 gctcagcgcc tgccgcaacg tcattcggct gacgcccaac gcgttggcga tatcgacctc
    14761 ggacggcaat ttgtcgccgg gcaccaacgt gcgggacacg atcagctgct cgagccaatt
    14821 cccgatgcgc gtatgcgcag gcagatctcc tgcgccggcc aggtcgggac gatccaccag
    14881 cagcggatca tccatcgtca atacgcgagt caccgtcagc cgtgccgggc gccgatccag
    14941 tgc
//
LOCUS       DASRJA010000121         4308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1596788,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000121 DASRJA010000000
VERSION     DASRJA010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596788"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VFC01_05515"
     CDS             <1..175
                     /locus_tag="VFC01_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZN79136.1"
                     /translation="SDQRSQAEALFDRLVAAAGPTGLLSEEYDPVAERALGNHPQAYS
                     HIGLLRCAQLLDT"
     gene            complement(233..1333)
                     /locus_tag="VFC01_05520"
     CDS             complement(233..1333)
                     /locus_tag="VFC01_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZN79137.1"
                     /translation="MSRSPAPRRRATLASLAAELKVSRTTISNAYNRPDQLSADLRER
                     VLATAKRLGYAGPDPVARSLRTRRAGAVGLMITEPLNYSFSDPAALDFVAGLAESCEE
                     AGQGLLLVAIGPNRSVSDGTAAVLAAGVDGFVVYSASDDDPYLPVVQQRHLPVVVVDQ
                     PKDVPGTSRVCIDDRGSMRQLAEYVVGLGHREIGLLTMRLGRDWPHGGPRPALADPER
                     VQTPHFHVQRERIHGVYDAMTAAGLDPESLTVVESYEHLPTSGGAAAEVAIDANPRIT
                     ALMCTADVLALSAMDYLRARGIYVPGQMTVTGFDGIPEAVRRGLTTVVQPSMEKGRRA
                     GRLLHHPPRDGLPVIDVLDTEVLRGRTSGPPA"
     gene            1426..2340
                     /locus_tag="VFC01_05525"
     CDS             1426..2340
                     /locus_tag="VFC01_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HZN79138.1"
                     /translation="MRAIRAAVGLALSTSLIVGVAGCGQDNKATSDHGAASIVASTNV
                     WGSVASAVTGDHASVKSIIDSPAEDPHSFEVTPADAAAIADASLVVYNGGGYDQWVDD
                     VLAGHPNVATVSAYSLLKPTEEPANEHVFYDIATAQLAATRIADRLAQADPDHADAYR
                     TNAAEFGKQAGAILQSERAIAQSHPGAAVVATEPVAHYLLVNSGIVDKTPEGFTKAIE
                     EDTDPSPADLAAVLDLIKNRQVSALVYNAQTQTAVTKQLQDAAQQASVPIVTVTETLP
                     AGIDYLTWQRQTVDQLASQLDKAAQTTR"
     gene            2350..3162
                     /locus_tag="VFC01_05530"
     CDS             2350..3162
                     /locus_tag="VFC01_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HZN79139.1"
                     /translation="MAADVVAELTGARLAFGDRVLWDHLDLTVRAGEFVAVLGPNGTG
                     KTSLLKVLLGQLPLSAGTVSVAGRRVEAGSERIGYVPQHRSVERGLSMRGQDLVGLGY
                     DGHRWGMTSLRERSAKHGAVQQALRQVNGVHLAGVPVGVMSGGEMQRIRIAQALATDP
                     VLLLCDEPLLNLDPANARLVSALIDQRRREADTAVLFVTHEVNPVLPFVDRVLYLVDG
                     RFRIGTVEEVMTSETLSELYRADIQVVKVGGRYVVVGEHLDHSGHAVGHDHD"
     gene            3155..4021
                     /locus_tag="VFC01_05535"
     CDS             3155..4021
                     /locus_tag="VFC01_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZN79140.1"
                     /translation="MTERLADLFGNLFAFNVTGDLLSRDFVQQALLAAALLALVSGLV
                     GPFIVMRQMSFAVHGSSELSLTGASFALLAGFNVGIGALVGCALAAVLFGILGQRARE
                     RDSAIGVVLAFGLGLAVLFIHLYPGRTGTSFALLTGQIVGVGYSGLALLSVVTVLVVA
                     VLAVSYRPLLFATVDPEVAAGRGVPVRALGIVFAALVGVTAAQGVQIVGALLVMSLLI
                     TPAAAAVRVFASPVATIAASVVFAEIAAVGGIVLSLAPGVPVSVFVTTISFAIFLVCW
                     LIGSRDRQPIAG"
     gene            4100..>4308
                     /locus_tag="VFC01_05540"
     CDS             4100..>4308
                     /locus_tag="VFC01_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HZN79141.1"
                     /translation="MNVSTTSHWIPDGSGPPLLERATVGGRLREVADEVPDRVALVEG
                     LPTPERRQWTYAELLADAERCARWLL"
BASE COUNT          666 a         1419 c         1463 g          760 t
ORIGIN      
        1 atccgatcag cggtcgcagg ccgaggcgct gttcgatcgg ctggtcgccg cagcggggcc
       61 gacggggctg ttgtccgagg agtacgaccc ggtggccgag cgggcgttgg gcaaccatcc
      121 gcaggcgtac agccacatcg ggctgctgcg ttgcgcgcaa ctcctcgaca cgtagttgcg
      181 atttcggtgc gctaacagac gctgtgagcg tacgtttgcg caccgaaatc actcaagcgg
      241 gcgggcccga ggtgcggccg cgcagcactt cggtgtcgag cacatctatc accggcagcc
      301 cgtcgcgagg cgggtggtgc agcagccgtc cggcgcggcg gcccttctcc atgctgggct
      361 gtacgacagt ggtcagcccg cgccgcaccg cctccgggat gccgtcgaac ccagtcaccg
      421 tcatctgtcc gggcacgtag atcccccgcg cgcgcaggta gtccatcgct gacaacgcga
      481 gcacatcggc cgtacacatc aacgccgtga tccgcggatt cgcgtcgatc gccacctcgg
      541 cggctgcccc gccggacgtc ggcaggtgtt catagctctc gaccacggtc agcgactcgg
      601 gatctaaccc ggccgcggtc atcgcgtcgt acaccccgtg gatgcgctcg cgctgcacat
      661 ggaaatgcgg cgtctgtacc cgctccgggt cggccagcgc aggccgcggc ccgccatgcg
      721 gccagtcgcg tcccagtcgc atggtgagca ggccgatctc ccgatgaccc agcccgacaa
      781 catattccgc gagctgccgc atcgaaccgc ggtcgtcgat gcatacccgc gaggtgccag
      841 ggacgtcctt gggctggtcg accacaacta cgggtagatg tcgctgctgg acgacgggca
      901 gatatggatc gtcatcggac gccgagtaga cgacgaagcc gtcgacaccc gcggcgagca
      961 cggccgccgt gccgtcgctc acgctccggt tcggtccgat cgccaccagc aacaagccct
     1021 gccccgcctc ttcacatgat tcggcaagtc cggcaacgaa atccagtgcg gcgggatcgg
     1081 agaacgaata gttcagcggc tcggtgatca tcaggccgac cgcgccggcc ctgcgggtgc
     1141 gcagcgaccg ggccaccgga tcgggtcccg cgtagcccaa ccgcttggcg gtggccagca
     1201 cgcgctcacg gagatcggcc gacaactggt ccggcctgtt gtacgcgttg gagatggtcg
     1261 tgcgcgagac cttgagctcg gccgccaacg aggcaagggt cgcccgccgc ctgggcgcgg
     1321 gactcctcga catgcattac gacgttagcg gatcgatttg gcgataccag gttgccgtgc
     1381 gctagagtta ttggaaacgg ttttcatttg tattaggggt gaacgatgcg tgcgatcagg
     1441 gcggctgtcg gtctggcgtt gagcacaagc ctgatcgtcg gtgtggccgg ctgcggccag
     1501 gacaacaaag ccacgtccga ccacggcgcg gcgtcgatcg ttgcgtcgac gaacgtgtgg
     1561 ggcagcgtcg ccagcgccgt caccggcgac cacgcgtctg tgaagtcgat catcgacagc
     1621 cccgccgaag acccgcactc gttcgaggtc acccctgccg atgcggccgc gatcgccgac
     1681 gcatcactgg tcgtgtacaa cggcggcggc tacgaccaat gggtcgacga cgtgctcgcc
     1741 ggccatccca acgtcgcgac cgtcagcgcc tactcgctgc tcaaacccac cgaagagccc
     1801 gccaacgagc acgtcttcta cgacatcgcc accgcccagt tggcggccac ccgcatcgcc
     1861 gaccggctgg ctcaggccga tcccgaccac gccgacgcgt atcgaacgaa tgccgcggag
     1921 ttcggcaagc aggccggcgc catcctgcaa tccgagcgcg cgatcgccca gtcgcacccg
     1981 ggggctgcgg tcgtggccac cgagcccgtc gcccactacc tgctggtcaa ctccgggatc
     2041 gtcgacaaga cgcccgaggg attcaccaag gcgatcgaag aggacaccga tccgtcgccc
     2101 gccgacctgg ccgcggtcct cgacttgatc aagaaccgtc aggtgtcggc gttggtgtac
     2161 aacgcgcaga cccagaccgc cgtcaccaaa cagcttcaag acgcagccca gcaggcgtcg
     2221 gtgcccatcg tcacggtgac cgagacgctt cccgccggca tcgactacct gacttggcag
     2281 cggcagaccg ttgaccagtt ggcgagccag ctcgacaagg ccgcgcagac aacccgatag
     2341 gttctggttg tggctgctga cgtcgtcgcc gaactgaccg gcgcccgatt ggcattcggc
     2401 gatcgcgtgc tttgggacca cctggacctg accgtgcggg ccggcgagtt cgtcgcggtg
     2461 ctcggcccca atgggacggg caagacctcg ctgctcaagg tgctgcttgg acagctgccg
     2521 ctcagcgcgg gcaccgtcag cgtcgcgggt cggcgagtgg aggcaggcag cgagcggatc
     2581 ggctatgtcc cgcaacaccg gtcggtggag cgcgggctgt cgatgcgggg gcaagacctt
     2641 gtcgggctgg gttacgacgg tcaccgctgg ggcatgacga gcctgcgcga gcggtcggcc
     2701 aaacacggtg ccgtgcagca ggcacttcgt caggtcaacg gcgtgcacct cgcaggtgtg
     2761 ccggtcggcg tgatgtccgg tggcgaaatg cagcgcatcc gcattgcgca ggcactggcg
     2821 accgatccgg tgctgctgct gtgtgacgag ccgctgctga acctcgatcc cgccaacgcc
     2881 cggctggtct ccgcgctgat cgaccagcgc cgccgcgagg ccgacaccgc cgtgctgttc
     2941 gtcacccacg aagtcaaccc ggtactaccg ttcgtggatc gggtgctgta tctcgtcgac
     3001 ggccgtttcc gcatcggcac cgtcgaagag gtgatgacct cggagacgct gtccgagttg
     3061 taccgagctg atatccaagt ggtgaaggtc ggcggccgct acgtcgttgt tggcgagcac
     3121 ctcgaccact ccggccacgc ggttgggcat gaccatgact gagcggctcg cggacttgtt
     3181 tggaaatctc ttcgcgttca acgtgaccgg cgatctgctg agccgcgact tcgtacagca
     3241 ggcgctgctc gcggccgcgt tactcgcgct ggtgtccggc ttggtcgggc cgttcatcgt
     3301 gatgcggcag atgtcgttcg cggtgcacgg ctccagcgaa ttgtcgctca ccggtgcgtc
     3361 tttcgcgctg ctggccggtt tcaacgtcgg gatcggtgcg ttggtcgggt gcgcactggc
     3421 ggcggtgctg ttcggcatcc tcggccaacg tgcacgcgaa cgtgattcgg cgatcggagt
     3481 cgtgctcgca ttcgggctag gccttgcggt gctgttcatc catctgtacc cgggccgtac
     3541 cggcaccagc ttcgcgttgc tgaccgggca gatcgtcggt gtcggctatt cggggttggc
     3601 gctgctgagc gtggtgaccg tgctcgtcgt cgcagtgctg gcggtctcct accgcccgtt
     3661 gttgttcgcg accgtcgacc ccgaagtggc ggccggccgc ggagttccgg tgcgggcgtt
     3721 gggaattgtg ttcgcggctc tggtgggcgt gaccgccgcg cagggtgtgc agatcgtcgg
     3781 cgcgctgttg gtgatgtcgc tgttgatcac gccggcggcc gccgcggttc gggtattcgc
     3841 ctcgcccgtc gcgacgatcg cggcatcggt ggtgttcgcg gagatcgcgg cggtcggtgg
     3901 catcgtgctg tcgctggcgc ccggtgtgcc ggtttccgtg ttcgtcacga cgatttcctt
     3961 cgcaatcttc ttggtgtgct ggctgatcgg aagccgcgac cgtcagccga ttgcaggctg
     4021 atttcgccgc taatcgccgc cgaagaacgt accgtttcct gtgaattgcc tggggaggcg
     4081 cagggccaaa gggggccgca tgaacgtttc gaccacgtca cattggattc ccgacggcag
     4141 cggaccgccg ctgctggagc gagcgacagt cggtggccgg ctgcgcgagg tcgccgacga
     4201 ggtgcctgac cgcgtcgccc tcgtcgaggg gttgccgacg ccggagcgac gccaatggac
     4261 ctatgcggaa ttgcttgccg acgcggagcg ctgtgcacgt tggctgct
//
LOCUS       DASRJA010000122        16573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4133031,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000122 DASRJA010000000
VERSION     DASRJA010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16573
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4133031"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFC01_05545"
     CDS             <1..369
                     /locus_tag="VFC01_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5130 family protein"
                     /protein_id="HZN79142.1"
                     /translation="DVSVTYPFPVKDLVFLDDALKYGSRAAKARFAVYLGDLGEDTAA
                     TAREILAEVPTPNNAVLLAVSPNQRAIEVVYGADVQGRGIEESAPLGVSAAAASFKEG
                     NLIDGLISAVRVLSSGVSPR"
     gene            complement(358..708)
                     /locus_tag="VFC01_05550"
     CDS             complement(358..708)
                     /locus_tag="VFC01_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HZN79143.1"
                     /translation="MAVNRSRKARAARRRKRRVSAVVNDLTAEEWAALKAAWKGCAYC
                     GAADGPLQRDCVMAISRGGRYTVDNVVPACASCNTSKCNEEVTTWLRRKRLDERAFLM
                     RYMQIRAALVTSAR"
     gene            complement(793..3378)
                     /gene="pepN"
                     /locus_tag="VFC01_05555"
     CDS             complement(793..3378)
                     /gene="pepN"
                     /locus_tag="VFC01_05555"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HZN79144.1"
                     /translation="MALPNLTREQAVERAALVTVDSYRITLDLTDGAGKPGEHTFHSV
                     TTVEFDALAGADTYIDIAADAIRSATLNGCTVDVSGYDESTGIPLQGLSEHNVLVVDA
                     DCRYSNTGEGLHRFVDPVDGEVYLYSQFETADAKRMFACFDQPDLKATFDIMVTAPLN
                     WRVISNGASGHVHGEGDAHVHTFVTTPRMSTYLVALIAGPYAHWADTYSDDHGDIPLG
                     LYCRSSLAEFMDHERLFTQTKQGFGFYHNNFGVPYAFGKYDQLFVPEFNAGAMENAGA
                     VTFLEDYVFRSKVTRASYERRAETVLHEMAHMWFGDLVTMQWWDDLWLNESFATFASV
                     LCQAEATEFTEAWTTFANVEKSWAYRQDQLPSTHPVAADIPDLAAVEVNFDGITYAKG
                     ASVLKQLVAYVGLEEFLSGLRDYFRDHAFANATFGDLLGALEKASGRDLSGWGRQWLK
                     TTGLNTLRPDFDLDSDGRITRFAINQSGAQPGAGETRVHRLAVGIYDDDGSGKLVRTH
                     REELDVEGSTTEVPALVGLSRGKLILVNDDDLTYCSLRLDPESLQTALTRIADIADPL
                     PRTLAWSAAWEMTREAELRARDFVSLVAGGVQAETEVGVAQRLLLQAQTALSAYADPQ
                     WAVADGWPAFADRLLELARGAEAGSDHQLAYVNALCGSLLSDRHTGVLSTLLDADPAS
                     QGLAGLEVDTDLRWRIVTALAAAGVVDSDGPDTPFIDTEAERDPTAAGQRHALEASTA
                     RPQAAVKESAWEEVIEDDTLPNVTARSMVGAFVRPGQHELLKPFRDRYFGAISGVWER
                     RSSEVAQTVVIGLYPSWDISQAGLEAADQFLADPDVPPPLRRLVLEGRAGVERSLRAR
                     AFDIT"
     gene            complement(3413..3802)
                     /locus_tag="VFC01_05560"
     CDS             complement(3413..3802)
                     /locus_tag="VFC01_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN79145.1"
                     /translation="MIKAKSTITATLVGVATAVTLAAPASADPTADNSFLNALTNAGI
                     GYNDPASTEQLGQSICPMLVEPGKSLASVYSTVSNNGINPDMAAFFTGIAISMYCPSA
                     MSSIGNGTFLNQLSGLNGLNGLGIPGL"
     gene            3978..4601
                     /locus_tag="VFC01_05565"
     CDS             3978..4601
                     /locus_tag="VFC01_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HZN79146.1"
                     /translation="MPEKSIADFWFDPLCPWCWITSRWILEVEKVRDIEVNFHVMSLA
                     VLNENREDLPEQYREGLKQAWKPVRVAIAAEQAKGQEILAPLYTAMGTRIHNQSYRDF
                     DSDFSTVIKEALEEVGLPAELAEAADTDKYDEALRKSHHEGMDAVGDDVGTPTIHVNG
                     VAFFGPVLSKIPRGEEAGKLWDASVTFASYPHFWELKRTRTEPPTFD"
     gene            complement(4620..5252)
                     /locus_tag="VFC01_05570"
     CDS             complement(4620..5252)
                     /locus_tag="VFC01_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZN79147.1"
                     /translation="MKTLVVSASPREDGNSHVLARSAIDGARSAGHDVQHVFLDEYVQ
                     RMLGNCRRCRSVDDGSCSLDDRYEELLVDHILPADGIIFAMPLYFYGMPARLKTVFDR
                     LFCYTANSAPLQDQVVSEVMDKRVAVLISCEESYVGATQGVIAQFQEITRYLRQDLVG
                     VVVGNANSRGEIVRDPSDPLSCARDLGARLFDVRVTDYRLDTARSNRVWA"
     gene            5334..6740
                     /locus_tag="VFC01_05575"
     CDS             5334..6740
                     /locus_tag="VFC01_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HZN79148.1"
                     /translation="MTVTLTEPTVQTDGTYRDQIVAVEPGGNEYIAEADRHGKPSQLF
                     WTWTSPNLEFATIFVGVLSVAVFGMTFWQAVLGIVIGTGLGAIAHYFLSARGPLHGVP
                     QMVLGRLPFGFKGNALPSVLMSVTAGVGWFATNSVSGAYALATLFGFGPLLGLVIIVL
                     LQTGLAFFGHNLVQAFERWTFPVLAVIFAIASVVILSKSHFGAPAGAGGVGGMGGFLL
                     TVGTAFGYAAGWTPYAADYTRYLPATVSTARTGFFAAAGLFVSCVFLEVVGAASVTIG
                     GDSLDNPTAAFTSQLASPLAKATLLAIAIGAVAANAINIYSGAMAFVTLGVKLPDHIA
                     RALVTVFFGVAGFLVAWWALPDAAQSYEAFLLIIAYWIGPWLGVMFADQYLRRGQTVA
                     SFLYDRSYANWTGLLSFVVGLVVSVALFSNQEKFVGWVAKAYPALGDVTFFVGFVLSG
                     AAYLVLCRSKIAAERAAV"
     gene            6741..7187
                     /locus_tag="VFC01_05580"
     CDS             6741..7187
                     /locus_tag="VFC01_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZN79149.1"
                     /translation="MDAAAMLEVAYQEARKGLAEGGIPIGAALFGADGVLLGSGHNRR
                     VQQGDPSVHAETDAFRAAGRQRDYRSTVMVTTLSPCWYCSGLVRQFNIGAVVIGESRT
                     FTGGHDWLAEHGVSVTVLDDERCVTMMTEFIAAKPELWNEDIGVSE"
     gene            7184..8137
                     /locus_tag="VFC01_05585"
     CDS             7184..8137
                     /locus_tag="VFC01_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HZN79150.1"
                     /translation="MIATVDISHWHEGGAQADAAAAAVDEGLQQAGFVLVTGHDVDPA
                     LAREVRAAARAFFSLPPSVKQRYSVPVAGHGWLAPGVEANAYAEGTETPPDLKESYSL
                     GAETTTGDPEVDRIWFAPNVWPDEVPSLQPLVHEYTNAMRRLADDLLALFAHALGLPE
                     NPFATLASRPTWTMNINHYPPVSVVGEPEPGQFRIGPHTDFGTVTILDREPGAGGLQV
                     YSDADGWSDAPYDPDALTVNIGDLLEYWSGRRWPSGRHRVQPPQPHAPEEDLVSLIYF
                     YEANHDAVVTPLPPPIGRVSGLEPVTSSAFIKERLDAITVA"
     gene            complement(8134..9156)
                     /locus_tag="VFC01_05590"
     CDS             complement(8134..9156)
                     /locus_tag="VFC01_05590"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005074679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HZN79151.1"
                     /translation="MKLQDFPRYPLTFGPSPIHQLDRLTKHLGGATIWAKRDDCNSGL
                     AFGGNKVRKLEYLVPDALAKGADTLVSIGGVQSNHTRQVAAVAAKLGLKCRLVQEKWV
                     DWDDPVNDKVGNILLSRIMGADVRLDPSGFDIGIRSSWEDALREVEEAGGKPYGIPAG
                     ASEHPLGGLGFANWAFEVAEQERQSGVYFDTIIVCTVTGSTHAGMIAGFAALQDTVDS
                     RPRRVLGIDASATLDKTRDQVARIARHTADLIELGRGLRDDEITVLEGWAGEYYGIPV
                     KSTVEAIKLCGSLEGMIIDPVYEGKSMAGLIDLVTSGEIPKDSTVLYAHLGGQPALNA
                     YSSIFD"
     gene            9210..9689
                     /locus_tag="VFC01_05595"
     CDS             9210..9689
                     /locus_tag="VFC01_05595"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016888270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HZN79152.1"
                     /translation="MRVYLGADHAGFELKQAIIEHLRNAGHEPIDCGAYVYDAEDDYP
                     AFCIAAAAKTLADPGSLGIVIGGSGNGEQIAANKVPGIRCALAWSVETATLAREHNNA
                     QVMGIGGRMHTLEQALEIIDAFLAAEWSKAERHQRRIDILAEYEKTHIAPPVPGAPA"
     gene            9692..10486
                     /locus_tag="VFC01_05600"
     CDS             9692..10486
                     /locus_tag="VFC01_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HZN79153.1"
                     /translation="MPEGHTLHRLARLHQRRFGRAPVMVSSPQGRFVDGAAAVNGRIL
                     KKATAWGKHLFHHYEGGRVVHVHLGLYGTFTEVPIPMALPVGQVRMRMIGTEYGTDLR
                     GPTVCEVIEEPEIADVVARLGPDPLRRDADGTLAWARITKSRRLIGALLMDQTVIAGV
                     GNVYRNELLYRHRIDPYRAGTHIDEAEFDAMWTDLVDLMKVGYRRGKIIVVRPEDDHG
                     LPSYGPGRPRTYVYRRAGEPCRVCGTTIRTAELEGRNVFWCPTCQK"
     gene            10509..12182
                     /locus_tag="VFC01_05605"
     CDS             10509..12182
                     /locus_tag="VFC01_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HZN79154.1"
                     /translation="MELILVVVGAIVVTAVAHRRGLEPALVLVVVGFAVSFAPEFEGI
                     ELESEVLLSVVLPPLLYSAALDFSFPTFLRNIRPILGLGVGLVVVTAFAVAGIASWLV
                     PSLTFATALILGAVVAPPDAVTAVAVGRKLGLPKKVMAILTGESLVNDAAALSLFSIA
                     VAQVAGSKAFISNPLLLFTYSAALGTAVGAALGFITLWIRRRLAHPGLETIQGLVVPF
                     AAFISAEHLHASGVLAVVVAGFVVGHGSLDAGYQTRLQERYVWNSVDVMLEAFVFAYI
                     GLQLRFVLEDLHQAHESLTEVALASGVVLVVVLLIRPACVFLMFGRGLLSRHVETRLS
                     VPVPVVGARGALGNRKRGKWSTLVDRRLLTWQENVVVSWTGMRGVVTLAAASGIPMMT
                     TAGQPFPERATIQAIAFAVSVGTLLIQGWTLPLLIRWLGPSFDGDRACDDAETRKAEQ
                     IVHGAADDVLARFAADPPAGLDAATLANIRLTIARHSQDADEMPDPESHTLRAEVFSQ
                     LYREVLSAQRAALITERDAGRIEDEAARAMLERLDLQEAGVSSRLESRF"
     gene            complement(12179..12469)
                     /locus_tag="VFC01_05610"
     CDS             complement(12179..12469)
                     /locus_tag="VFC01_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HZN79155.1"
                     /translation="MAAYVISEVEVLDQESFEQYRNLAKASIEEHGGRYLVRAALPEA
                     AEGDWPSQRRLVILEFADMETARRWHSSESYAKALAIRDRALSRRLIFVEGV"
     gene            complement(12475..13308)
                     /locus_tag="VFC01_05615"
     CDS             complement(12475..13308)
                     /locus_tag="VFC01_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HZN79156.1"
                     /translation="MNGVLLVLIIVVIAAIALAVYSSSKASGRRNAASLADAKADARR
                     LIERLGGQVLNLTGADDASKQALADASERYTAASSQIDQATTVRQAALAKESAIEGLY
                     YARAARVAMGMDPGPELESLAGQSSAGAVTEDRRINFEGREIEASPTPSQRTPNYYPG
                     GRVAGRPVPAGWYSEPWWKPALVAGAWGLGSVLLFDALFTGMHGVDYGAQGFESGYGD
                     GYSDGFAAGQDGGVDGGGADASGFDGYSGDGGGGWDGGGWDSGGDFGGGDFGGGDFGG
                     F"
     gene            complement(13354..14547)
                     /locus_tag="VFC01_05620"
     CDS             complement(13354..14547)
                     /locus_tag="VFC01_05620"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN79157.1"
                     /translation="MLDESEVPINGLCAPGFERVRDAFESNFTDGDDVGAAVAVWVEG
                     DLVVNLWGGFADAAGRRPWREDTLASIYSGTKGLTSTCVHLLADRGELDLNAPVTRYW
                     PEFGQAGKRDITVAMVLGHRSGVIGPRNRLHWADATDWDAVCEHIAAAEPWWEPGTAQ
                     GYHMVTFGFILGEVVRRVTGRTIGQYLRTEIAEPLGIDVHIGLPKQEHARCAEMINKP
                     HIRDVLADGDAPEHPESLTEHPMAGMAIAMGFVPDDELGSNALDVWRTLEFPATNAHV
                     SALGMATFYNALAQEKLLSREHMELVRVSQGGFDTDVVLGSRVADHGWGLGYMLNQRG
                     VAGPNLRTFGHGGSGGSFAFVDLEHRIGYAYVMNYFDATKCNADPRSVVLSDEVYSAL
                     GVIRD"
     gene            complement(14655..15371)
                     /locus_tag="VFC01_05625"
     CDS             complement(14655..15371)
                     /locus_tag="VFC01_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79158.1"
                     /translation="MQHGAIRGGLVDLLHALHDGRYSRIVVVAHGVGTYISYDALTAF
                     WAQTHGLHARPSAWQDLRDQGNPWRITDFVTVGAPMALADLLLTRPTLASGFKKSDAV
                     RRRELFEGLVRRGVLVRCPPRSESLPVDRNAVLGSHSPFAVTRWTNLWFPVVRGELRG
                     DWFGGALRPLFGPGIRDIEILGNEPERFKRGSAHTEYFAHPDKDDEGDAAWHIRQTLA
                     LQTLTVLKHLLNAPPPQPGN"
     gene            15422..15724
                     /locus_tag="VFC01_05630"
     CDS             15422..15724
                     /locus_tag="VFC01_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79159.1"
                     /translation="MVLFTNALAAMRYRPNQKTKMTALVIKPMIHTGTSVLSAYPVTN
                     SAGIARSIARTTIHTTRQIPNRESGTLLGRRRSSRSVIGIAPANLLAVIRNDQWYS"
     gene            16110..>16573
                     /locus_tag="VFC01_05635"
     CDS             16110..>16573
                     /locus_tag="VFC01_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,3-beta-glucanase"
                     /protein_id="HZN79160.1"
                     /translation="MGELNRRNLMLMTGFGALAAAIPVPEGRASAGRGPAPAAPPGGA
                     AQTYLFRDEFDGPAGSAPDASKWETALAREDMEDPTFWELPENVGQYRDDRRNVFLDG
                     KSNLVFRAAKDGNTFYSGKLFGKYRGPIGTTWEARIKLNCLTPGCWPAWYLA"
BASE COUNT         2719 a         5394 c         5519 g         2941 t
ORIGIN      
        1 gacgtgtcgg tgacctaccc gttcccggtc aaggacctgg tgttcctcga cgatgcgctg
       61 aagtacggct cacgcgccgc caaggctcgc ttcgccgtct acttgggcga tctcggcgag
      121 gacaccgccg ccacggcccg cgagattctg gccgaggtgc cgacgccgaa taatgcggtg
      181 ctgcttgcgg tttcgccgaa tcagcgcgcg atcgaagtgg tctacggcgc cgatgtgcag
      241 ggtcgcggga tcgaggagtc cgcgccgctc ggagtgtcgg cggccgccgc gtcgttcaaa
      301 gagggcaacc tgattgacgg cctgatcagc gccgtccgcg tgctcagctc tggcgtctca
      361 ccgcgctgac gtaacaaggg ccgcacggat ctgcatgtag cgcatcaaga atgcgcgctc
      421 gtcgagccgc ttccggcgta gccaggtggt gacctcttca ttgcacttgc tggtgttgca
      481 agatgcgcac gcgggcacga cgttgtcgac tgtgtatcgc ccgccccgcg agatcgccat
      541 gacgcagtcg cgctgcagcg gtccatcagc agcgccgcag taggcgcatc ccttccaggc
      601 ggccttgagt gctgcccact cttcggcggt gaggtcgttg acgaccgcgc ttacccgtcg
      661 ctttcgccga cgggcagccc gcgccttgcg actccgattg accgccatcc gaaaagcgta
      721 tgggcgatga gcgtgcgtga aatgtacgtc cgacgcggcg tgtcggcgta cagacacgca
      781 cggccgcggt agttaggtga tgtcgaaggc gcgggcgcgc aatgagcgct cgacacccgc
      841 gcggccctcg agcaccagcc tccgcagcgg cggcggcaca tccgggtcgg ccaagaactg
      901 atccgccgcc tccaggcccg cctggctgat gtcccacgac gggtacagcc cgatgacgac
      961 ggtctgtgcg acctcgctcg aacgccgctc ccacacgccg gaaatcgcgc cgaagtacct
     1021 gtcgcggaac ggctttagca actcatgctg gcccggtcgc acgaacgcgc cgaccattga
     1081 ccgcgcggtg acgttgggca gcgtgtcgtc ctcgatcacc tcttcccatg cggactcctt
     1141 caccgcggcc tggggtcgtg ccgtggacgc ctccaacgcg tgccgttgac ccgccgcggt
     1201 cgggtcccgc tcggcctcgg tgtcgatgaa cggcgtgtcg gggccgtcgg aatcgacgac
     1261 gcccgccgca gccagcgcgg tcacgatccg ccaccgcaga tccgtgtcca cctcgaggcc
     1321 ggccagaccc tgcgaagccg gatccgcatc caacagcgtg ctcaacacgc cggtgtgcct
     1381 gtccgacagc agcgagccgc acagcgcatt gacgtacgcc agctggtgat cggaacctgc
     1441 ctcggcgccg cgggccaact ccagcaaccg gtcggcgaag gcaggccaac cgtccgcaac
     1501 cgcccattgc gggtcggcgt aggcgctcag cgccgtctgc gcctgcaaca gcagccgctg
     1561 tgccacaccg acctcggtct cggcctgcac gcccccggcg accaacgaca caaaatcgcg
     1621 agctcgaagt tctgcttcgc gggtcatctc ccacgccgcc gaccaggcca gcgtgcgggg
     1681 caacgggtcg gcgatgtcgg cgatgcgggt aagcgctgtt tgcaacgact cagggtcaag
     1741 ccgtagcgag cagtacgtca ggtcgtcgtc gttgacgagg atcagtttcc cgcgcgaaag
     1801 gccaaccagc gcaggaactt cggttgtcga gccttcgacg tcgagctcct cgcgatgggt
     1861 gcggaccaac ttgccgctgc cgtcgtcgtc gtaaatgccg accgccagtc ggtggacccg
     1921 cgtctcaccg gcgcccggct gcgccccgct ctgattgatc gcgaagcggg tgatccgccc
     1981 gtcgctgtcg aggtcgaagt cgggccgcag tgtgttgagc ccggtggtct tgagccactg
     2041 ccgcccccag ccggacaggt cgcggcccga cgccttttcc agcgcaccga gtaggtcacc
     2101 gaacgtcgcg ttggcgaagg cgtggtcgcg gaagtaatcc cgcaggccgg acaggaactc
     2161 ctccagcccg acgtaggcaa ccagctgctt gagcacgctg gcgcccttgg cgtaggtgat
     2221 gccgtcgaag ttcacctcga cagcggccag gtccgggatg tcggcggcta ccggatgcgt
     2281 cgagggcaac tggtcctggc ggtaggccca cgacttctcc acgttggcga atgtcgtcca
     2341 cgcctcggtg aattcggtcg cctcggcctg gcacagcacc gaggcgaagg tcgcgaacga
     2401 ctcgttgagc cagaggtcgt cccaccactg catggtgacg agatcaccga accacatgtg
     2461 ggccatctcg tgcagcacgg tttcggcgcg ccgctcgtag gacgcgcgag tgaccttgct
     2521 gcggaagacg tagtcctcca ggaaggtcac cgcgcccgcg ttttccattg cgccggcgtt
     2581 gaattcggga acgaacagct ggtcgtactt gccgaatgcg tacggtacgc caaaattgtt
     2641 gtggtagaag ccaaatccct gcttggtctg ggtgaagagt cgttcatggt ccatgaactc
     2701 cgccagcgat gagcggcagt acaggccgag tgggatgtcg ccgtgatcgt cggaatacgt
     2761 gtcagcccag tgcgcatacg gccctgcgat cagcgcgacg aggtatgtgc tcattcgcgg
     2821 tgtggtgacg aaggtgtgca catgtgcatc accctcgcca tggacgtgtc cgctggcgcc
     2881 gttagagatg actcgccagt tcagcggggc ggtcaccatg atgtcgaacg tggccttcag
     2941 gtccggctgg tcgaaacacg cgaacatccg cttggcgtcg gcggtttcga actgtgagta
     3001 caggtagacc tcgccgtcga ctgggtcgac gaaccggtgc aggccctcgc ccgtgttgga
     3061 gtagcggcag tcggcgtcga ccacgagaac gttgtgttcg gacaggcctt gcagcgggat
     3121 cccggtcgat tcgtcatacc cggacacgtc gacggtgcac ccgttgagtg tggcgctgcg
     3181 aatcgcgtcg gcggcgatgt cgatataggt gtcggcgccc gccagcgcgt cgaactcgac
     3241 cgtcgtcacc gagtggaagg tgtgttcgcc gggtttaccg gcgccgtcgg tcaggtccag
     3301 ggtgatgcga tagctgtcga cggtgacgag cgcggcgcgt tcgacggcct gctcgcgggt
     3361 gagattggga agtgccacgc gaccaaccta tcggccgcgc tcgctagcgg ccctatagcc
     3421 caggtatccc gagcccgttc aggccgttca gcccgctcag ctggttcaag aacgtcccgt
     3481 tgccgatcga ggacatcgcc gacgggcagt acatcgatat cgcgatgccg gtgaagaacg
     3541 ccgccatgtc ggggttgatg ccgttgttcg acacggtgga gtagacggaa gcgagtgact
     3601 tgcccggctc cacgagcatc gggcagatcg actgtccgag ttgttcggtg cttgccggat
     3661 cgttgtagcc gatgccggca ttggtgaggg cgttgaggaa ggaattgtcg gcggttggat
     3721 ctgcggatgc cggcgccgcg agcgttaccg ccgtcgcgac cccgaccaaa gtcgcggtaa
     3781 tcgtggattt cgctttaatc acaaaagaaa cactggtgta caacggtcac attgacaaca
     3841 cggctccata accatttgca cactatgcgt gtcctgtgcc ccggctattc gaactccgtg
     3901 gcagtgggaa cacgatcgtt gagtgcacgg ttgtgctgaa cggattaccg accttcgatc
     3961 gagaggacca agcaggaatg cccgaaaagt ctattgcgga tttctggttc gacccgctgt
     4021 gcccgtggtg ctggatcacg tcgcggtgga tcctcgaggt cgagaaggtc cgcgatatcg
     4081 aggtcaactt tcacgtcatg agcctggcgg tgctcaacga gaacagggag gacctgcccg
     4141 agcagtaccg cgaaggcctc aagcaggcgt ggaagccggt gcgcgtggcg atcgccgctg
     4201 agcaggccaa gggccaggag attctggcgc cgctctacac cgcgatgggc acccggatcc
     4261 acaaccagag ctatcgagac ttcgactccg atttctcaac tgtcatcaag gaggcgctcg
     4321 aagaggtcgg gctgcccgcc gagttggccg aggcggccga caccgacaag tacgacgagg
     4381 cgctgcgcaa gagccaccac gagggcatgg acgcggtcgg cgacgacgtc ggcaccccga
     4441 ccatccacgt caatggtgtc gcgttcttcg gcccggtgct ctcgaagatc ccgcgcggcg
     4501 aggaagccgg caagctgtgg gatgcctcgg tgacgttcgc gtcctacccg cacttctggg
     4561 agctcaagcg cacccgcacc gagccgccga ccttcgactg acggcgcctg ccgcgaacgt
     4621 caggcccaga cgcggtttga ccgcgcggtg tccagccggt aatcggtgac ccggacgtcg
     4681 aacagccgcg cgcccagatc ccgcgcgcat gacagcggat cgctggggtc gcgcacgatc
     4741 tcgccgcggc tgttcgcgtt gccgaccacg acgccgacga ggtcctgccg gagataccgg
     4801 gtgatctcct ggaactgtgc gatcacgccc tgtgtcgcgc cgacgtagct ctcctcgcat
     4861 gagatgagca cagcgacgcg cttgtccatg acttcgctga ccacttggtc ctgcagcggt
     4921 gcgctgttcg cggtgtagca gaacagccgg tcgaagactg tcttcaaccg ggcaggcatg
     4981 ccgtagaagt acagcggcat cgcgaagatg atgccgtcgg ccggcaggat gtggtcgacc
     5041 agcagctctt cgtaacggtc gtcgagtgag cagctgccgt cgtcgacgct cctgcagcgt
     5101 cggcagttgc ccagcatgcg ttgcacgtac tcgtccagaa agacgtgttg gacgtcgtgg
     5161 cccgccgagc gggcgccgtc gatcgccgat cgggcaagaa catgggagtt gccgtcttcg
     5221 cgcgggctcg cgctcaccac gagtgtcttc acgtggctac caacttgctt tgaagccctt
     5281 ttgtttccgg cgcgtttact ccggttgcgg gggatggtcg cgggcatgac ggtatgacgg
     5341 tgacactgac cgaacccacc gtgcagaccg acggcaccta ccgcgaccag atcgtcgcgg
     5401 tggaaccggg cggcaacgaa tacatcgccg aggcggaccg ccacggcaag cccagccagc
     5461 tgttctggac ctggacctcg cccaatctgg agttcgcgac gatcttcgtc ggcgtgctgt
     5521 ccgttgccgt cttcggcatg accttctggc aggcggtgct cggcatcgtc atcggcaccg
     5581 gcctgggcgc catcgcgcac tacttcctgt ccgcgcgcgg cccgctgcac ggggtgccgc
     5641 aaatggtgct gggccgactt ccgttcgggt tcaagggaaa tgcgttgccc tcggtgctga
     5701 tgtcggtcac cgcgggtgtc ggctggttcg ccaccaacag cgtgagcggc gcctacgcgc
     5761 tggccacgct gttcgggttc gggccgctgc tcgggctggt catcatcgtg ctgctgcaga
     5821 ccggcctggc cttcttcgga cacaacctgg tgcaggcgtt cgagcgctgg acattcccgg
     5881 tgctcgccgt gatcttcgcg atcgcctcgg tggtcatcct gtcgaagtcg cacttcggtg
     5941 cacccgccgg cgccggcggt gtcggcggca tgggtggatt cctgctcacc gtcggcactg
     6001 cattcgggta cgcggccgga tggacgccgt acgccgcgga ctacacccgc tacctgcccg
     6061 caaccgtgtc gaccgctcgc accggtttct tcgccgccgc ggggctgttt gtgtcgtgcg
     6121 tgttcctgga ggtcgtcggc gcggcatcgg tcaccatcgg cggcgattcg ctggataatc
     6181 cgaccgcggc gttcaccagc cagctcgcct cgccgctggc caaggcgaca ttgttggcca
     6241 tcgcgatcgg tgccgtcgcg gccaacgcga tcaacatata ttccggcgca atggctttcg
     6301 tgaccctggg tgtcaagctg cctgaccaca tcgcaagggc gctcgtcacc gtgttcttcg
     6361 gtgtggcggg gttcctggtg gcctggtggg cattgcccga cgccgcgcaa agctacgaag
     6421 cgttcctgtt gatcatcgcc tactggatag ggccatggct cggcgtgatg ttcgccgacc
     6481 agtacctgcg ccgggggcag accgtcgcca gcttcctgta cgaccgctcg tatgcgaact
     6541 ggaccggctt gctgtcgttt gtcgtcgggc tggtggtgtc ggtggcgttg ttctccaatc
     6601 aggagaagtt tgtcgggtgg gtggccaagg cgtacccggc gctcggcgac gtcacgttct
     6661 tcgtcggatt cgtgctgtcc ggcgctgcgt atctggtgct gtgccggtcg aagatcgccg
     6721 cggagcgggc tgccgtctag atggacgccg ccgcgatgct cgaggtcgcc taccaggagg
     6781 cccgaaaagg cttggcggag ggcggcattc cgatcggtgc cgcgttgttc ggcgccgacg
     6841 gtgtgttgct cggcagcggt cacaaccggc gggtccagca gggcgacccg tcggtgcacg
     6901 ccgaaaccga cgcgtttcgg gcggccggac gccaacgcga ctatcggtcc acggtgatgg
     6961 tgacgacgct gtcgccatgc tggtattgca gcgggctggt gcgccagttc aacatcggcg
     7021 ccgtggtgat cggcgagagc cggacattca ccggcgggca tgactggctg gccgaacacg
     7081 gcgtgtcggt cacggtccta gacgacgagc gatgcgtgac aatgatgact gagttcatcg
     7141 ccgccaagcc ggaactgtgg aacgaagaca tcggagtaag tgagtgattg caaccgttga
     7201 catctcgcac tggcacgagg gtggcgccca agctgacgcc gccgcggccg ccgtcgacga
     7261 gggattgcag caagcgggat tcgtcctcgt caccggacac gacgtcgacc cggcattggc
     7321 gcgcgaggta cgcgcggccg cccgcgcgtt cttcagcctg ccgccgagcg tcaagcagcg
     7381 gtactcggtg ccggtggccg ggcatggttg gctcgcgccc ggggtcgagg ccaatgccta
     7441 cgccgaaggc accgagacgc cgccggatct caaggaaagc tacagccttg gcgccgagac
     7501 aacgaccggc gatcccgagg tcgaccgaat ctggttcgcg cccaacgttt ggcccgacga
     7561 ggtaccgtca ttgcagccgt tggtgcacga gtacaccaac gccatgcgcc gattggccga
     7621 cgatctgttg gcgttgttcg cccatgcgct cgggctgccg gaaaacccgt tcgccacgct
     7681 ggccagcaga cccacgtgga ccatgaacat caatcactac ccgccggtga gcgtggtcgg
     7741 cgaacccgag ccaggccagt tccgcatcgg cccgcacacc gacttcggca ccgtgacgat
     7801 cctcgaccgc gaacccggcg ccggcggcct gcaggtgtat tcggacgccg acggctggag
     7861 cgatgcgccg tatgaccccg acgcgttgac cgtgaacatc ggtgatttgt tggagtactg
     7921 gagcgggcgg cgctggccgt cgggccgcca tagagtgcag cctccccaac cgcacgcgcc
     7981 ggaggaggac ctggtctcgc tgatctactt ctacgaggca aaccacgacg cggtcgtcac
     8041 gccgctgccg ccgccgatcg ggcgggtgtc ggggctggag cctgtgacgt cgtcggcatt
     8101 catcaaggag cggctcgacg ccatcaccgt ggcttagtcg aagatgctcg agtaggcgtt
     8161 gagcgccggt tggccgccta ggtgggcgta gaggaccgtg gagtcctttg gaatctcacc
     8221 cgaggtgacg aggtcgatga gtccggccat cgacttcccc tcgtagaccg ggtcgatgat
     8281 catgccctcc agcgagccgc acagcttgat cgcttcgact gtcgacttga ccgggatgcc
     8341 gtagtactcg cccgcccagc cctcgagaac ggtgatctcg tcatcacgca gaccacggcc
     8401 gagctcgatg aggtcggccg tgtggcgggc tattcgcgca acttggtcgc gggtcttatc
     8461 gagggtcgcg gacgcgtcga tgccgagtac ccggcgaggg cggctgtcga cggtgtcctg
     8521 cagggccgcg aagccggcga tcatgccggc gtgtgtagag ccggtcactg tgcagacgat
     8581 gatcgtgtcg aagtacacgc cggactgtct ttcctgctcg gcgacctcga acgcccaatt
     8641 ggcgaagccg agtccgccga gcgggtgctc ggacgcgccg gccgggatgc cgtacggctt
     8701 cccgccggcc tcctcgactt cgcgtagtgc gtcctcccag gagcttcgga tgccgatgtc
     8761 gaagccggac gggtccagcc gcacatccgc acccatgatg cgtgacagaa gtatgttgcc
     8821 gaccttgtcg ttgacggggt cgtcccaatc gacccacttc tcctggacga ggcggcattt
     8881 caggccgagc ttcgccgcga cggccgcgac ctggcgggtg tggttcgatt ggaccccgcc
     8941 gatcgacacc agcgtgtcag ctcccttggc gagcgcgtcg gggacaaggt attcaagctt
     9001 gcggaccttg tttccaccga aggcgagccc tgaattgcag tcgtcgcgtt tggcccagat
     9061 ggtcgcacca ccgagatgct tggtgagtct gtcaagctgg tgaatggggc tcggcccgaa
     9121 ggtgagtggg tagcgcggga agtcttggag tttcatgatg cgcctccttc cgccaagtat
     9181 cctgccgtgc gtctgccaga atgaccgcca tgcgcgtcta cctgggagct gaccacgcag
     9241 gcttcgagct gaagcaggcc atcatcgagc acctccgcaa cgccggccac gagccgatcg
     9301 actgcggggc ctacgtctac gacgcggagg acgactaccc ggcgttctgc atcgccgccg
     9361 cggcgaagac cctcgccgat cccggcagcc tcggcatcgt gatcggcggc tcgggcaacg
     9421 gcgagcagat cgccgcgaac aaggttccag gtatccgctg tgcgctggcc tggagtgtcg
     9481 agaccgcaac gctggcccgc gaacacaaca acgcgcaagt gatgggcatc ggcggccgca
     9541 tgcacaccct cgagcaggcg ttggagatca tcgacgcttt cctcgccgcc gaatggtcga
     9601 aagccgaacg gcaccaacgc cgtatcgaca tcctcgccga gtacgagaaa acccacatcg
     9661 cgccgccggt gcccggcgcg cccgcctagg aatgccggaa ggccacaccc tgcaccggct
     9721 cgcccggctg catcagcgtc gattcggccg tgccccggtc atggtgtcca gcccgcaggg
     9781 cagattcgtc gacggcgccg cagcggtcaa cggacggatc ctgaagaagg ccaccgcctg
     9841 gggcaaacac ctgtttcacc actacgaagg cggccgtgtg gtgcacgttc acctggggct
     9901 gtacggcacc ttcaccgagg tccccatacc gatggcgctg ccggttggac aggtccgcat
     9961 gcggatgatc ggcacggaat acggcaccga cctgcgcggc ccgacggtgt gcgaggtgat
    10021 cgaggagccc gagatcgccg acgtggtggc tagactcggc ccggacccgt taaggcgcga
    10081 cgccgacggc acgctggcct gggcgcgaat caccaagtcc cgcaggctga ttggtgcact
    10141 gttgatggac cagacggtga tcgccggggt cggcaacgtg tatcgcaacg agctgttgta
    10201 ccggcaccgc atcgacccat accgcgcggg cactcatatc gacgaggccg aattcgacgc
    10261 gatgtggacc gacttggtcg acctgatgaa ggtcggctat cgtcgcggaa aaatcatcgt
    10321 cgtccggccc gaggacgacc acggcctgcc gtcctacggc cccggccgac cacggaccta
    10381 tgtgtaccgc cgcgcgggtg agccatgccg ggtctgtggc accacgatcc gcaccgcgga
    10441 actggagggc cgcaacgtgt tctggtgtcc gacctgccag aagtaggtta ggacgcgaca
    10501 atcggcgggt ggaactgatc ctcgttgtcg tcggcgccat cgtggtgacg gccgtcgcgc
    10561 atcggcgcgg cctcgaaccc gcgctcgttc tcgttgtcgt cggattcgcg gtgtcgttcg
    10621 cacccgaatt cgaaggcatc gagctggaat ccgaagtgct gctgtcggtg gtgctgccgc
    10681 cgctgcttta ctctgcggcg cttgacttct cgttcccgac gttcctgcgc aacatccggc
    10741 cgatcctcgg tctcggcgtc gggctcgtcg tggtcaccgc attcgccgtc gccggtatcg
    10801 cctcgtggct ggtgccctcg ctcacgttcg ccaccgcgct gatcctcggc gcggtggtgg
    10861 cgccgcccga cgctgtcacc gctgtggccg tgggtcgcaa actcgggctg cccaagaagg
    10921 tgatggcgat cctgacgggg gagagcctgg tcaacgacgc ggcggcgctg tcgctgttct
    10981 ccatcgcggt ggcacaggtc gcgggcagca aggccttcat ctccaatccg ctgctgctgt
    11041 tcacctacag cgccgcactg ggcacggccg tcggggcggc gctcggcttc atcacgctgt
    11101 ggatccgccg tcggctcgcg catcccggcc tggaaaccat ccagggtttg gtggtgccgt
    11161 tcgccgcctt catctcggcc gaacacctgc acgcctcggg cgtgctggcg gtggtggtcg
    11221 cgggcttcgt ggttggccac ggatcgctcg acgccggcta tcagacgcgg ctgcaggagc
    11281 gctacgtgtg gaactccgtc gacgtgatgc tcgaggcatt cgtgttcgcc tacatcgggc
    11341 tgcagctgcg cttcgtgctc gaggatctcc accaggcgca cgagtcgctg acggaggtgg
    11401 cgctggcgtc cggtgtggtg ttggtggtgg tgctgttgat ccgtccggca tgtgtcttct
    11461 tgatgtttgg ccgcggcctg ttgtcgcgtc acgtcgagac gcggctcagc gtgcccgttc
    11521 cggtcgtcgg tgcgcgcggc gcgctcggca accgaaaacg aggcaaatgg tcgacccttg
    11581 tcgaccgccg tctgctgaca tggcaggaga acgtcgtcgt gtcgtggacg ggcatgcgcg
    11641 gcgtggtgac tttggctgcg gcgtcgggca ttccgatgat gaccaccgcc gggcagccct
    11701 tccccgagcg ggcgacaatt caggccatcg cgttcgcggt cagcgtcggc acactgctaa
    11761 ttcagggctg gacgctgccg ctgttgatcc gttggctcgg gccgtcattc gatggggacc
    11821 gcgcgtgcga tgacgccgag acccgcaagg ccgaacagat cgtgcacggc gccgcagacg
    11881 atgtgctggc gcggttcgcc gctgatccgc cagccgggct cgacgccgcg acgctggcca
    11941 acatccggtt gacgatcgcc aggcattcgc aggacgccga cgagatgccc gacccggagt
    12001 cgcacacgct gcgcgccgag gtgttctcac agctgtatcg cgaagtgctg tcggcgcaac
    12061 gggctgcgct catcaccgaa cgcgacgccg gccgtatcga ggatgaggcg gcgcgggcca
    12121 tgctcgagcg gctcgacctc caggaggccg gcgtctcgtc gcgcctggaa agccgcttct
    12181 agacgccttc gacaaagatg aggcgccggc tcagcgcgcg gtcgcggatg gcgagcgcct
    12241 tggcgtagga ctccgacgag tgccaccgcc gcgcggtctc catgtccgcg aattcgagga
    12301 tgaccagccg tcgttgcgac ggccaatctc cttccgccgc ttcgggaagc gccgcccgca
    12361 cgaggtagcg cccgccgtgt tcctcgatcg acgccttagc gaggttgcgg tattgctcga
    12421 agctttcttg gtcgagcact tcgacttcgg agatgacgta cgcggccatc gcgctcagaa
    12481 gccgccgaag tctccgccgc cgaaatcgcc gcccccgaag tcaccgccgc tgtcccagcc
    12541 gccgccgtcc cagccgccgc cgccgtcgcc gctgtaaccg tcgaagccgg aggcgtccgc
    12601 gccaccaccg tcgacaccgc cgtcctgccc ggccgcgaac ccgtcggagt acccgtcgcc
    12661 gtagccgctc tcgaaaccct gtgcgccgta atcaactccg tgcatgccgg tgaacagcgc
    12721 gtcgaacaac agcaccgaac ccaggcccca cgcgccggcc accagggccg gtttccacca
    12781 tggctctgaa taccagccgg caggcaccgg gcggccggcg acgcgaccac cggggtagta
    12841 gttcggggtg cgttgcgacg gtgtcggcga cgcctcaatt tcgcggccct cgaagttgat
    12901 tcgccggtcc tcggtcaccg cgcccgccga cgactgcccg gccagcgact ccaactccgg
    12961 ccccgggtcc atacccatcg cgacgcgagc tgcgcgcgcg tagtagaggc cttcgatagc
    13021 actttccttg gcgagggccg cctgtcgcac ggtggtggcc tggtcgatct gtgacgaggc
    13081 tgcggtgtag cgctcggagg cgtcggccag cgcctgcttg gaggcgtcgt cggcgccggt
    13141 caggttgagc acttgcccgc cgagccgttc gatgagccga cgggcatcgg cctttgcgtc
    13201 ggccagcgag gccgcgttgc gcctgcccga agccttcgat gagctgtata cggccaacgc
    13261 gatggcggcg atgaccacga tgatgagcac gagcaagacg ccgttcatgg cgtccagcgt
    13321 acagagagga aagcccccaa ttagggctgc gaatcagtcg cgaattacgc ccagagctga
    13381 gtacacctca tcggagagca cgacgctgcg cgggtcggca ttgcacttcg tcgcgtcgaa
    13441 gtaattcatc acgtacgcgt agccgatgcg gtgctcgagg tcgacgaacg cgaacgagcc
    13501 acccgacccg ccatggccga acgtccgcag gttcgggcct gcgacgccgc gttgattgag
    13561 catgtagccc aggccccagc cgtggtcggc gacccgcgag cccagcacga cgtcggtgtc
    13621 gaaaccgcct tgcgacaccc ggaccaactc catgtgctcg cgcgacagca gcttctcctg
    13681 ggccagcgcg ttgtagaacg tcgccatgcc gagtgccgat acgtgtgcgt tggtggcggg
    13741 gaattcgagc gtgcgccaca cgtcgagcgc gttggagccc agctcgtcgt cggggacgaa
    13801 tcccatggca atagccatgc ccgccatcgg atgttcggtc agcgactccg ggtgttcggg
    13861 cgcgtcaccg tccgccagta cgtcgcggat gtgcggcttg ttgatcatct cggcgcagcg
    13921 ggcgtgctct tgcttcggca gaccgatgtg cacatcgatg ccgagcggtt cggcgatctc
    13981 ggtgcgtagg tactggccga tggtgcgacc ggtcacgcgg cggaccacct cgccgaggat
    14041 gaacccgaac gtcaccatgt gatagccctg cgcggtgccg ggctcccacc acggttcggc
    14101 cgccgcgatg tgttcgcaca ccgcgtccca gtcggtcgcg tcggcccagt gcagccggtt
    14161 ccgcggcccg atgacgccgg accgatgccc cagaaccatg gcgacggtga tgtcccgctt
    14221 gcctgcctgg ccgaactccg gccaataccg cgtcaccggg gcgttcaggt cgagctcgcc
    14281 gcggtcggcc agcagatgca cgcaggtgct ggtcagcccc ttcgtgccgg agtagatgct
    14341 ggccagcgtg tcctcccgcc atggccgccg tcccgcggca tcggcgaagc cgccccagag
    14401 gttgacgacg aggtcgcctt ccacccagac ggcgaccgcg gcgccgacgt catcgccgtc
    14461 ggtgaagttg gattcgaaag cgtcgcgcac ccgctcgaag ccgggagcgc acaatccgtt
    14521 gatggggacc tcggactcgt cgagcaagca tcctcctgcc ggtccaagtc cccgattggt
    14581 ttggctgcgc cttacggtag ccgagcaacg ccgtggtcag cgcgaacgtc acacacctgt
    14641 gacgatcttc acggctagtt cccaggctgt ggaggcggtg cgttcaacag gtgcttgagg
    14701 acggtaaggg tctgcagcgc gagcgtctga cgtatgtgcc aggccgcgtc gccctcgtcg
    14761 tccttgtcgg gatgggcaaa gtattcggtg tgcgccgaac cgcgtttgaa cctttcaggc
    14821 tcgttcccaa gtatttcgat gtcgcggatg cccggaccga acagcgggcg tagtgcgccg
    14881 ccgaaccagt cgccgcgcag ctcgccgcga accaccggaa accagaggtt cgtccagcgt
    14941 gtgacggcga agggggagtg ggaaccaagc acggcgttcc tgtcgacggg cagcgattcg
    15001 gaccgtggcg ggcaccgcac cagcacgccg cgacgtacca gcccctcgaa cagttcgcgc
    15061 ctgcgcacgg cgtcggactt cttgaatccg ctggcgagcg tgggccgggt gagtaaaagg
    15121 tcagcaaggg ccattggtgc gccgacagta acgaaatcgg ttatgcgcca cgggtttccc
    15181 tggtctcgaa ggtcctgcca cgcagacggt ctggcgtgta gcccgtgcgt ttgggcccag
    15241 aacgcagtca gcgcgtcgta ggagatgtag gtgccgacgc cgtgggccac cacgacgatg
    15301 cgcgagtagc gaccgtcgtg aagtgcatgc aacaggtcga ccaatccgcc tcgaatggcg
    15361 ccgtgctgca tatgagtacg gcgacgtgtc caggtagcgg gcgacgtcga ccaaggaggt
    15421 cgtggtcttg ttcaccaacg ccctcgccgc catgcggtac aggccgaacc agaagaccaa
    15481 gatgactgca ctggtgatca agccgatgat ccacactgga acgtccgtgt tgagcgcgta
    15541 tccggtgacg aacagtgcgg ggatcgccag aagcattgcc aggaccacga tccacaccac
    15601 gcgccagatc ccgaataggg agtcaggcac attactgggc cgacgcagga gcagccgcag
    15661 cgtcatcggc atcgcaccgg caaatttgtt ggcggtcatc aggaatgacc agtggtactc
    15721 gtagatctcg gcttgggctt gcggttgggc gaagcggcga gcttcgtagg aatccgttat
    15781 ctcagcgggt agcgagtagt agtcccattt cttctcgccc tcggcggcat tggggcgaag
    15841 tgccgtcttc gcaaatccgt cgagagtctc cgtcggatgc ttctcgccca tgccgtggac
    15901 gatcaccacg gcggtacgga ccgtttcgtc ggctgccatg tcgtccgagt ctgagggttg
    15961 gccgcgccca atcggacagg ttttcgatga ttgacgccgg cgttcacccg atttcttagc
    16021 cctgtcgtgc tggccctacc aggcgtcaac ataggtgcgt accactcccg cgcggcgcgc
    16081 gcgttcggcc gcatggttgt atccgaagga tgggtgagtt gaatcgtcgc aatctgatgt
    16141 tgatgacggg gttcggtgca ttggccgccg cgatccctgt gccggagggc cgagcctcgg
    16201 cgggtcgtgg tccggcaccc gccgcgccgc caggtggcgc ggcccagacg tacctgttcc
    16261 gggatgagtt cgatggcccg gccggctcgg cccccgatgc atcgaaatgg gaaacggcac
    16321 tggcccgcga ggacatggaa gacccgacgt tctgggagtt gcccgagaac gtcggccaat
    16381 accgtgacga tcggcgaaac gtcttcctcg acggcaagtc caaccttgtc ttccgcgcgg
    16441 ccaaggacgg caacacgttc tacagcggca agctcttcgg caagtacaga ggacccatcg
    16501 gcaccacctg ggaagcccga atcaagctga actgcctcac tcccggttgt tggcccgcct
    16561 ggtacttggc caa
//
LOCUS       DASRJA010000123         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3566993,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000123 DASRJA010000000
VERSION     DASRJA010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3566993"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..960)
                     /locus_tag="VFC01_05640"
     CDS             complement(46..960)
                     /locus_tag="VFC01_05640"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HZN79161.1"
                     /translation="MPHKASVAIVGSGNISTDLLYKLLRSEWLEPRWMVGIDPESEGL
                     ARARKLGLETSHEGVDWLLGLDEKPDLLFEATSAYVHRDAAPRYAEAGIRAIDLTPAA
                     VGPAVIPPANLREHLDAPNVNMITCGGQATIPIVYAVSRVVDVPYAEIVASVSSVSAG
                     PGTRANIDEFTKTTSKGVETIGGAKRGKAIIILNPAEPPMIMRDTIFCAIPEDADHDA
                     IAQSIRDVVAEVQTYVPGYRLLNEPQFDEPSVVNGGNHVVTTFVEGEGAGDYLPPYSG
                     NLDIMTAAATKVGEEIAKESLAVTGGQA"
     gene            complement(973..1758)
                     /locus_tag="VFC01_05645"
     CDS             complement(973..1758)
                     /locus_tag="VFC01_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HZN79162.1"
                     /translation="MLSVQVRDQLAADLAQAERSRVPISPLTDGNPDIDVVDAYEIQL
                     INIRQRVAEGARVVGHKVGLSSEAMQKMMNVDEPDYGHLLDEMQVYEDRPVQSANYLY
                     PRVEVEVGFILADDLPGAGCTEDDVLAATAAFAPSIELIDTRITDWKIKLCDTIADNA
                     SSAGWVLGEARVSPKDIDIKSIDAVLTNNGEVVAKGRSDAVLGNPVTAVAWLARKVEG
                     FGVRLKAGDIVLPGSCTRAIDAPPGSNFVADFAGLGSVRLSFE"
     gene            1832..>2360
                     /locus_tag="VFC01_05650"
     CDS             1832..>2360
                     /locus_tag="VFC01_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN79163.1"
                     /translation="MTGQEYDVVVVGSGAAGMVAALTAAHQGLSTVVVEKAPHYGGST
                     ARSGGGVWIPNNEVLKRDGVADTAEAARKYLHTIIGDVVEPERIDTYLERGPEMLSFV
                     LKNSPLKMCWVPGYSDYYPETPGGRPGGRPAGRSIEPKPFNARKLGADLGGLEPPYGK
                     VPLNVVVMQQDYVRLN"
BASE COUNT          407 a          763 c          750 g          440 t
ORIGIN      
        1 cggtgatccg gatatcccag atcggattga agaagatgtc ggtgctcatg cctggccccc
       61 tgttactgcc agtgattcct ttgcgatttc ctcgccgacc ttggtggccg ccgctgtcat
      121 gatgtccagg tttcccgaat acggcggcaa gtagtcgcct gcgccctcgc cttcgacgaa
      181 cgtcgtgacg acgtggttgc caccgttgac caccgacggc tcgtcgaact gcggctcgtt
      241 gagcagccgg tagccgggca catacgtctg cacctcggcg acaacgtccc tgatggactg
      301 cgcgatcgcg tcgtggtcgg catcctcggg gatcgcgcag aagatggtgt cgcgcatgat
      361 cattggcggt tcggcggggt tgaggatgat gatcgccttg ccgcgcttcg cgccaccgat
      421 ggtctccacg cccttgctcg tggtcttggt gaactcgtcg atgttggcgc gggtgcccgg
      481 ccccgcagaa accgaagaca ccgaggccac aatttccgcg tacggcacat cgacgacgcg
      541 cgacaccgca tagacgatcg gaatcgtggc ctgcccgccg caggtgatca tgttgacgtt
      601 cggtgcgtcg agatgctcac gcaggttggc cggcgggatg acggcggggc caaccgccgc
      661 gggcgtcagg tcgatggcac ggatgccggc ctcggcatag cgaggcgccg catcgcggtg
      721 cacgtaggcg ctggttgctt cgaacagcag gtcgggcttc tcgtcgagac cgagcagcca
      781 gtcgacacct tcgtggcttg tttccagccc gagcttgcgt gcgcgtgcga gcccttcgga
      841 ctccgggtcg atgccgacca tccagcgcgg ctcgagccac tccgagcgca acaacttgta
      901 cagcaggtcg gtgctgatgt tgcccgatcc gacgatcgca acggaggcct tatggggcat
      961 gggtactcct cgttattcga aactcagtcg tacagaacct aacccggcga agtccgcaac
     1021 gaaattgctg ccgggcggtg catctatcgc acgtgtgcat gagcccggca acacgatgtc
     1081 gccggccttg agacgcacgc caaacccttc caccttgcgg gccaaccacg ccactgcggt
     1141 cacagggttg ccgagcaccg catcgctgcg gcccttggcg acgacttcgc cgttgttggt
     1201 caacaccgcg tcgatggact tgatgtcgat gtccttcggc gacacccgtg cctcccccag
     1261 cacccagccg gccgaggagg cgttgtcggc gatggtgtcg cacagcttga tcttccagtc
     1321 ggtgatgcgg gtgtcgatca actcgatgga aggagcaaag gcggccgtcg ccgcgagcac
     1381 gtcgtcctcg gtgcagccgg ctccgggcag gtcatcggcc aggatgaagc ccacctcgac
     1441 ctcgacgcgc ggatacaagt agttggccga ctgcaccggg cggtcttcat acacctgcat
     1501 ctcatcgagc aagtgcccgt agtcgggttc gtccacattc atcatttttt gcatggcctc
     1561 ggacgacagg ccaaccttgt gtcccaccac gcgggcaccc tctgcgaccc gctgacggat
     1621 gttgatcagc tggatctcgt aggcatcgac cacgtcgata tcggggttgc catcggtcaa
     1681 cggggagatc gggacgcggc tgcgctcggc ctgagcgagg tcggcagcca gctggtcacg
     1741 cacctggacg ctgagcatct atgcgaagtc ccctcgtttc gtcgaccggc gcagttgtgg
     1801 gtcggccagg caattctata acgtgttcta catgacgggt caggagtacg acgtcgtcgt
     1861 ggtgggaagc ggcgcggccg gcatggtcgc cgccctcacc gcggcccacc agggactatc
     1921 cacagtagtc gtcgagaagg cgccgcacta tggcggttcc actgcgcggt ccggcggtgg
     1981 cgtatggatt ccgaacaacg aggtcctcaa gcgcgacggc gtcgcggaca cggccgaggc
     2041 cgcccgcaaa tacctgcaca cgatcatcgg cgacgtggtc gaacccgagc gcatcgacac
     2101 ctacctcgag cgcggcccgg agatgttgtc cttcgtcctg aagaactcgc cgctgaagat
     2161 gtgctgggtc cccggctact ccgactacta tccggaaaca ccgggcggac ggcccggcgg
     2221 tcgcccagcg ggtcgttcga tcgagccgaa gccgttcaac gcccgcaagc ttggcgccga
     2281 tcttggcggc ctcgaaccgc cctacggcaa ggtcccactc aatgtcgttg tgatgcaaca
     2341 ggactacgtg cggctcaacc
//
LOCUS       DASRJA010000124         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2439021,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000124 DASRJA010000000
VERSION     DASRJA010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2439021"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VFC01_05655"
     CDS             <1..793
                     /locus_tag="VFC01_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HZN79164.1"
                     /translation="RNEFERQWNPHSPAPDLLVGVEAVVHLAGATIAGRFTDDHKAAI
                     RDSRVEPTRRLADAAALADDGPATFVSASAVGFYGFDRGDTLLGEESMRGDGFLADVV
                     ADWEAATAPAAQAGLRVVNVRTGIVQSARGGTLKLMRPLFAAGLGGRLGSGEQWLSWI
                     GLDDLLDVYHRALYDGRLTGPVNAVAPEPVRNADYTRALAAVLHRRAVVPVPSVGPRL
                     LLGEQGARELAQANQRVVPSKLETLGHRFRRSKLTDALAHELGRC"
     gene            complement(772..1506)
                     /locus_tag="VFC01_05660"
     CDS             complement(772..1506)
                     /locus_tag="VFC01_05660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZN79165.1"
                     /translation="MSGVAPKVLFIVNEHLATEALLGEAFTDCGFDVDTFEVVPAERV
                     DNPAGDVTFPDPTGYDVIVPLGARWPVYDDALRRTWVGAETQLVRDAADAGVALLGVC
                     FGGQLLAEAFGGSVARSTVPEIGWYDVATDNPDLVPGGPWFQWHFDRWTLPPGATEIA
                     RTANASQAFVLDRALALQFHPEIDLKLLDLWLDDDRDGEVVGAGLHHDELRDRTIELA
                     DDAAARVRGLVHGFLKHVASQQRPSS"
     gene            complement(1506..2738)
                     /locus_tag="VFC01_05665"
     CDS             complement(1506..2738)
                     /locus_tag="VFC01_05665"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015287811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN79166.1"
                     /translation="MPAVRRLLAVICALVMFTGCAPGALPTETETTTTDTAKADAVMR
                     IVRDVMAEAHLKAVIVRVTVDGREVVTEAVGESMTGVPATPAMHFRNGAVAISYVSTL
                     LLKLVDEKKVSLDDKLSKWLPEIPHADRVTLGQLAQMTSGYVDYVIGNTAMNEALYAN
                     PFRRWTVNEMLSYAVSKPLLYEPGTNWNYAHTNYVLLGLALEKATGEEMSKLLSDKVL
                     RPLGLTNTANSLTPEIPAPVLHAFTSERRAFLKIPAGTPFYEESTFWDPSWTITHGAI
                     QTTNIYDMEATAAGIGSGRLLTADSYHKMVSTDLRGKTHKQPGCTTCDEMNDAYTYGL
                     GIVISGNWLVQNPLFGGYGAIEAYLPSQKIAIAAAVTFAPEAFDDQGNYRNEADPLFR
                     KIAAELAPDDAPPMPPKK"
BASE COUNT          473 a          867 c          968 g          526 t
ORIGIN      
        1 gcgcaatgag tttgagcgcc aatggaatcc gcacagcccg gcacccgatt tgctggtcgg
       61 cgtcgaagcc gtcgtccacc tggcaggcgc gacgatcgcc ggccgtttca ccgatgacca
      121 taaggccgcg atccgagaca gccgtgttga gccgacacgc agactcgccg acgccgcggc
      181 actggccgac gacggtccag cgacatttgt cagcgcgtcg gcggtcggct tctacggctt
      241 cgaccgcgga gacacgctgc tcggtgaaga aagcatgcga ggcgacggct ttctggccga
      301 cgtggtggcc gactgggagg cggcgacggc gccggccgcc caggcggggc tgcgagtggt
      361 caacgtgcgc acgggaattg tgcaatcggc ccgcggtggc acgctgaagt tgatgcgccc
      421 gctgttcgcg gccggactgg gcgggcggct aggcagcggt gagcagtggt tgtcgtggat
      481 cgggctcgac gacctgctcg atgtgtacca ccgcgccttg tatgacgggc gattgacggg
      541 tcccgtcaat gcagtcgcgc cggaaccggt gcgcaacgcc gactacacca gggcgctcgc
      601 ggcggtgctg caccgacggg cggttgtgcc ggtgccatcg gtgggtccgc ggctgttgct
      661 gggggagcag ggtgcacgcg agctcgccca ggccaatcag cgggtggtac cgagcaagct
      721 ggagacgctg ggccaccggt tccggaggtc aaagctcaca gacgcgctgg ctcacgaact
      781 cgggcgctgc tgactcgcga catgctttag gaagccgtgc accaggccgc gcactcttgc
      841 cgcggcgtcg tctgcgagtt caattgtgcg gtcgcgtaat tcgtcgtggt gcagccccgc
      901 gccgacgact tccccgtcgc ggtcgtcgtc cagccacaaa tcgagcagct tcaagtcgat
      961 ctctgggtga aattgcagcg ccaacgcgcg gtcaaggacg aatgcctgcg acgcgttcgc
     1021 ggtcctggca atctcggtgg cgccgggcgg caacgtccac cggtcgaaat gccattggaa
     1081 ccacggcccg ccgggcacca ggtccgggtt gtcggtggcg acgtcgtacc aaccgatctc
     1141 gggcacggtc gatcgcgcca ccgagccgcc gaacgcctcg gccagcagtt gaccgccgaa
     1201 gcacacgccg agcagtgcga cacccgcgtc ggcggcgtcg cgcaccagtt gtgtctcggc
     1261 gccaacccag gtgcgacgca gcgcgtcgtc gtacacgggc cagcgcgctc cgagcgggac
     1321 aatgacgtcg tagcccgtcg gatcggggaa ggtcacgtcg ccggcggggt tgtcgacgcg
     1381 ttcggcgggc accacctcaa aggtgtcgac gtcgaaaccg cagtccgtga acgcctcgcc
     1441 cagcagggct tcagtcgcga gatgttcgtt gactatgaac aggaccttcg gcgccacgcc
     1501 cgacattatt tctttggtgg catgggtggc gcatcgtcgg gggcgagttc ggcagcaatc
     1561 ttgcggaaca gcgggtcggc ttcgttgcgg taattgccct ggtcatcgaa tgcttccggg
     1621 gcgaacgtga cagcggcggc gattgcgatc ttctgcgaag gtaagtaggc ctcgattgcg
     1681 ccgtacccac cgaacaacgg gttctgtacc agccagttgc ccgagatgac gattccgagg
     1741 ccgtacgtgt aggcgtcgtt catctcatcg caggtggtgc aaccgggctg tttgtgggtc
     1801 ttgccgcgaa gatcggtgga taccatcttg tggtacgagt cggcggtgag cagccgtccg
     1861 gatccgatgc ccgccgctgt cgcctccatg tcgtagatgt tcgtggtctg gatcgcgccg
     1921 tgggtgatgg tccaggacgg atcccaaaac gttgattctt cgtagaacgg tgtgcccgca
     1981 ggaatcttga ggaaggcgcg gcgctcagag gtgaacgcgt gcagcaccgg cgccgggatc
     2041 tcgggtgtca gtgagtttgc cgtgttcgtc agtcccagcg gccgaagcac cttgtccgac
     2101 aacagcttcg acatctcttc accggtcgcc ttctccagcg caagcccgag cagcacgtag
     2161 ttggtatgag cgtagttcca gttggtgccg ggctcgtaga gcagcggctt ggatacggcg
     2221 tagctcagca tctcgttgac tgtccagcgc cggaacgggt ttgcgtacag tgcctcgttc
     2281 atcgcggtgt tcccgatgac gtaatcgacg tagcccgaag tcatttgggc gagctggccc
     2341 agggtgactc tatcggcgtg cggtatctcg ggcagccact tcgacagctt gtcgtcgagg
     2401 cttaccttct tctcgtcgac gagcttgagc agcagtgtgg agacatacga gatcgcgacg
     2461 gcaccgttgc ggaagtgcat cgccggggtc gcagggacgc cggtcatcga ttcgccgaca
     2521 gcctcggtga caacctccct gccgtcgacc gttacccgga cgatcaccgc cttcagatgt
     2581 gcctccgcca tgacatcgcg aacgattcgc atcacggcgt cagctttggc ggtgtcggtg
     2641 gttgtggttt cagtctcggt cggcagtgcg ccgggtgcgc agccggtgaa catcactagg
     2701 gcgcagatca ccgcgaggag gcgccgtacg gcgggcatgc ccgatcttga cagagtgccg
     2761 cgccggattg tgatttttgg gtatccaacg acgtgtcgaa aatgaccgac tggttcctca
     2821 cagacgccga acgc
//
LOCUS       DASRJA010000125         3326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1871174,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000125 DASRJA010000000
VERSION     DASRJA010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871174"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFC01_05670"
     CDS             <1..398
                     /locus_tag="VFC01_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009475115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZN79167.1"
                     /translation="YRARGMSAEEATGHAATVLNNLAKSGAHTQLARPDADRHEAVGT
                     GLRAAISSFTFFATGALIPVLPYLFGLQGTIAVVVAASLVGVALLATGVIVGLLSGGP
                     PLRRGLRQLMIGYGAAAVTYLLGLLFGTG"
     gene            complement(447..1985)
                     /locus_tag="VFC01_05675"
     CDS             complement(447..1985)
                     /locus_tag="VFC01_05675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HZN79168.1"
                     /translation="MTGSTALNESRRATELAALADGEALDVIVIGGGITGTGIALDAA
                     TRGLRVALVEKHDLAFGTSRWSSKLVHGGLRYLATGNVGIARRSAVERGILMTRNAPH
                     LVKAMPQLVPLLPEMNTASRALVRFGFAAGDGLRKLAGTPSSTLPRSRRIDAARAIEM
                     APTVRRDGLSGGLLAYDGQLIDDARLVTAVARTAAQHGARILTRVGASNATGTSVTLT
                     DQRSGESFDVSARAVINAAGVWAGQVDTSIRLRPSRGTHLVFDAKSFGHPVAALTVPI
                     PGEINRFVFAMPEQLGRVYLGLTDEDAPGSIPDVPQPTSEEISFLLDTVNTALGVALG
                     PADVIGTYAGLRPLIDTGGGRTADVSREHAVVESGSGVISVIGGKLTEYRFMAEDVLD
                     RAVTLRGFAAGPCRTRNLPLVGAPSNPVATLRSPADLPGSLVARYGAEAPNVIAAASC
                     ERPTEPVADGIDVSRAEFEYAVTHEGAMTVDDILDRRTRIGLVAVDRDRAASAAEELL
                     TRHA"
     gene            complement(1982..2560)
                     /locus_tag="VFC01_05680"
     CDS             complement(1982..2560)
                     /locus_tag="VFC01_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN79169.1"
                     /translation="MVSIRNSQQTVEARILDAAAECVLAYGVERMTLTDIARRARVSR
                     PTIYRRWPDIRYVLADLLTARIAGLLDAIPSRGVGREDMVERIVAAAEYLRGDEVVMS
                     VLHNAPNMAMVYITERLGTSQQILIDALAHAIKLGQDEGSVRPGDPRHLAAMCLLITQ
                     SVIQSAQMVAPILDSKALGVELAHSLNGYLKP"
     gene            2578..>3326
                     /locus_tag="VFC01_05685"
     CDS             2578..>3326
                     /locus_tag="VFC01_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN79170.1"
                     /translation="MTRPDDQLLPPMKWNAWGDPAEAKPLSEGIRSLLTQALGIEESA
                     TAELEPEQVRLRPSALSPADREGLASIVGAEYCGSDDRARLLRAGGKSTLDLLRRKDS
                     GVQDAPDAVLLPGGEDEITAILRYCADRNIAIVPFGGGTSVVGGLDPIRGDFKAVVSL
                     DLRRLDQLHSLDEVSGEAELGAGLTGPEAERLLGERGFSLGHFPQSFEFATIGGFAAT
                     RSSGQDSAGYGRFNDMVRGLRTVTPAGVLDLG"
BASE COUNT          498 a         1130 c         1154 g          544 t
ORIGIN      
        1 tctatcgggc acgcggcatg agcgccgagg aggcaaccgg tcacgcggct acggtgctga
       61 acaacctcgc caagagcggc gcccataccc agctcgcccg gcccgatgcc gaccgacacg
      121 aggccgtggg cacgggtctt cgcgcggcga tttccagctt tacctttttc gcaaccgggg
      181 cgctgattcc cgtgctgcct tacctgtttg ggctgcaggg caccatagcg gtcgtagtgg
      241 cggccagcct ggtcggcgtc gcattactcg ctaccggagt cattgtgggc ctactgtccg
      301 gtgggccccc gctgcgccgt gggctgcgac agctgatgat cggctacggc gccgcggccg
      361 tcacctactt gctggggctg ctattcggca ccggctagcg cgtcgcgccg ccaccggggt
      421 cgcgaccacg tgggtgaccg gcacggttat gcgtgccgcg tcagcaactc ctccgcggcg
      481 gatgccgcgc ggtcccgatc taccgccacg agcccgatgc gggttcggcg gtccaggatg
      541 tcgtcgacgg tcatcgcacc ttcgtgggtg acggcgtatt cgaattccgc ccggctgacg
      601 tcgatgccgt cggcgaccgg ctccgtcggc cgctcacagc tggcggccgc gatcacgttc
      661 ggtgcttcgg cgccgtagcg ggctaccagc gaccccggca ggtcggcggg tgagcgcagc
      721 gtcgccaccg ggttcgacgg ggctccgacc agcggcaggt tgcgggtgcg gcatgggcct
      781 gccgcgaatc cccgcagcgt gaccgcgcgg tcgaggacgt cctcggccat gaaccggtat
      841 tcggtcagct tgccaccgat cacgctgatc acgccggagc ccgactcgac gacagcgtgt
      901 tcccgcgaca cgtcggccgt gcggcctccg cccgtatcga tcaacggccg cagcccggcg
      961 tatgtgccga tgacgtcggc cgggccgagc gcgacgccca acgcggtgtt gaccgtgtcg
     1021 agcagaaacg agatttcctc cgaagtcggt tgcggcacat cgggaatcga tcccggcgcg
     1081 tcctcatccg tcagccccag gtagacccgt cccagctgtt cgggcatcgc gaacacgaac
     1141 cggttgatct cgccgggaat cggaacggtc agggcagcaa cgggatggcc gaacgacttt
     1201 gcatcgaaca cgagatgtgt tccgcgactt ggccgcaatc ggatcgaggt gtccacctga
     1261 cccgcccaca cgccggcggc gttgatgacg gcgcgcgccg aaacatcgaa cgattcaccg
     1321 gagcgctgat cggtcaacgt caccgaggtg cccgtcgcgt tcgatgcgcc gactcgggtc
     1381 aggatgcgcg cgccgtgctg cgcggcggtg cgggccaccg cggtgacgag ccgcgcgtcg
     1441 tctatgagtt ggccgtcgta ggcgagcagg ccgccgctca acccgtcgcg gcgcacggtc
     1501 ggtgccatct cgatggcgcg cgccgcgtcg atccgccggg atcgcggcag cgtcgacgac
     1561 ggcgtgcccg ccagcttgcg cagcccgtcc cccgccgcga aaccgaagcg caccagagcc
     1621 cttgacgccg tgttcatttc gggcagcagg ggaaccagtt gcggcatggc tttgacgagg
     1681 tgaggagcgt tgcgggtcat aaggatgccg cgttcgaccg cgctgcggcg cgcgatgccg
     1741 acattgccgg tggcgaggta tcgcagcccg ccgtgcacca gcttggagct ccaccggctg
     1801 gtgccgaacg cgaggtcgtg cttttcgacc agcgcgaccc gcagcccgcg ggtcgcggcg
     1861 tcgagagcga tgcccgtgcc ggtgatgccg ccgccaatca cgatcacgtc gagcgcctcg
     1921 ccgtcggcca acgccgccag ctcggttgcg cgtcgggact cgttgagtgc tgttgagccg
     1981 gtcacggttt gaggtatccg ttcagtgagt gggcgagttc gacacccagc gccttcgagt
     2041 cgaggatcgg cgcaaccatc tgggcggact ggatcaccga ctgggtgatc agcaggcaca
     2101 tcgccgcgag gtggcgcggg tcgcccggtc gcacactgcc ttcgtcctgg ccgagcttga
     2161 tcgcatgcgc cagcgcgtcg atcagaatct gctggctggt gcccagccgc tcggtgatgt
     2221 acaccatcgc catgttgggg gcgttgtgca gtaccgacat caccacctca tcgccgcgca
     2281 ggtattcggc ggccgcgacg atgcgctcga ccatgtcctc acgcccaaca ccacgactcg
     2341 gtatcgcgtc gagcagaccg gcgatgcgtg cggtcagcag gtcggcgagc acataacgga
     2401 tatcgggcca gcggcggtag atggtcgggc ggctgacccg ggcgcgccgc gcgatgtcgg
     2461 tcagcgtcat ccgctcaacc ccgtacgcca gcacgcactc cgccgcagcg tcgaggattc
     2521 gcgcctcgac cgtctgctga gagttacgga ttgacaccat gtgtaatact gtaacgcatg
     2581 acgcgtccgg atgaccagct cctaccccca atgaagtgga acgcatgggg tgatcctgcg
     2641 gaggcgaaac cgctgtcaga gggcattcgc tcactgctca cgcaggcgct cggcatcgag
     2701 gagtcggcga cggccgagct cgagcccgag caggtcaggc tgcggccgtc cgcgctgtcg
     2761 cctgcggacc gcgaggggtt ggcgtcgatc gtcggcgccg agtactgcgg tagcgacgac
     2821 cgcgcccggc tactgcgcgc gggcggcaag tccaccctgg acctgttgcg ccgcaaggat
     2881 tccggcgtgc aggacgcgcc cgacgcggtg ttgctgcctg gcggcgaaga cgagatcacg
     2941 gcgattcttc ggtactgcgc ggaccgcaac atcgcgatcg tcccgttcgg cggcggcacc
     3001 agcgtggtcg gcgggctgga tccgatccgc ggcgacttca aggccgtggt gtcgctggac
     3061 ctgcgccggc tggaccagct gcactcgctc gacgaagtgt ccggcgaggc cgagttgggc
     3121 gctggactga ccggacccga ggctgaaagg ctgctcggcg agcgcggctt ctcgctcggc
     3181 cacttcccgc agagcttcga gttcgcgacg atcggcggat tcgcagcgac ccggtcgtcg
     3241 ggccaggact ccgccggtta tggccgcttc aacgacatgg tccgcggcct gcgcacggtc
     3301 acgcctgccg gtgtgctcga cctcgg
//
LOCUS       DASRJA010000126         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2438523,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000126 DASRJA010000000
VERSION     DASRJA010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2438523"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VFC01_05690"
     CDS             complement(<1..574)
                     /locus_tag="VFC01_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HZN79171.1"
                     /translation="MTYDGLSPEELQAETGIALPDKEVVSILDLNVDVDVLLDAASPI
                     DLAAAAQLNVGAALDAAAGASVLAFDSTAHADVQQETMITQILHADAIAESNQVSDIN
                     QTDAAEPLPPPTDDGTTPELGLFEGPLLNVDVDVDVDADLAAPIAGAVAANANVAAPI
                     SASVGANVITWNSETTAFSSQTAIINQSLEG"
     gene            complement(853..1320)
                     /locus_tag="VFC01_05695"
     CDS             complement(853..1320)
                     /locus_tag="VFC01_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79172.1"
                     /translation="MDRSLNPRLTRLDLLVGDWELTATSGGRIMSRSRSTFHWLGDRG
                     FLVQRNDPPTYLVPEWVDAAPRWVDAVIGLDDHSDVFTMLYTDSRGVCRTYRMHLDAG
                     RWTLESRPGRDFFQRFEGAFDDGRTVIDGRWEASDDGENWSTDFDVTYRRMAG"
     gene            1415..2170
                     /locus_tag="VFC01_05700"
     CDS             1415..2170
                     /locus_tag="VFC01_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HZN79173.1"
                     /translation="MTTDVGHAPFLLLSIRAEDAAADDEYAAMMRFAGLDTNGMRRIR
                     LTHEPLGDIDLDDWSGIILGGGPYNVSDPADSKSQTQRRVESELLPLIGRIVDDDFPF
                     LGCCYGVGTLGSVVGASVDRTYTEPVGALTVTLTEDGRADPLFAELPDAFDAYGGHKE
                     GASSLPSDLACLAYSADCPIQAFRVGDNVYATQFHPELDAGGICTRIDVYKDYGYFEP
                     ETADSLKSAALQRNIEHPPTILRRFRERYARPV"
     gene            2437..2889
                     /locus_tag="VFC01_05705"
     CDS             2437..2889
                     /locus_tag="VFC01_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZN79174.1"
                     /translation="MTPAPDYADQLRAADLRVTRPRLAVLEAVHSHPHADTETIFGAV
                     RSDLPDVSRQAVYDVLAALTGVGLVRKIQPSGSVARYESRVGDNHHHVVCRSCGVIAD
                     VDCAVGDAPCLTPSEHDALDGFVLDEAEVIYWGLCPDCSAAQASRSHP"
     gene            2930..>3440
                     /locus_tag="VFC01_05710"
     CDS             2930..>3440
                     /locus_tag="VFC01_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HZN79175.1"
                     /translation="MSESENPAISAPTAKSGRPHTNQDWWPDQVDLSVLHKHNPQATP
                     LGDDYNYAEEFKKLDVEAFKRDVIEVLTTSQDWWPADFGHYGPLMIRLSWHAAGTYRI
                     EDGRGGGGEGAQRFAPLNSWPDNASLDKARRLLWPVKQKYGRKISWADLLIFAGNVAY
                     ESMGFKTFGF"
BASE COUNT          630 a         1067 c         1099 g          644 t
ORIGIN      
        1 cgccttcgag cgactggttg atgatcgcgg tctgtgacga gaacgcggtc gtctcggagt
       61 tccacgtgat gacgttggcg ccgacgctgg cgctgatagg cgcggccacg ttggcgttgg
      121 cggccacagc gccggcgatc ggtgccgcca gatcggcgtc gacatcgacg tcgacgtcca
      181 cgttgagcag cgggccctcg aagagcccga gctccggggt ggtgccgtca tcggtgggcg
      241 gtggcagcgg ttcggccgca tcggtttggt tgatgtcgct cacctgattc gattcggcga
      301 tcgcgtcagc atgcagaatc tgggtgatca tggtttcctg ctggacatcg gcgtgggccg
      361 tggagtcgaa cgccagcacg ctcgcgccgg cggcggcgtc gagggccgct cccacgttca
      421 actgcgctgc ggcagccaga tcgatcggcg atgccgcgtc gagcagcaca tccacgtcga
      481 cgttgaggtc gagaatcgat acgacctcct tatcgggaag tgcgatgccg gtttcggcct
      541 gtagctcctc ggggctcagt ccgtcgtagg tcatggccgc ctctcctgtt cgttcccgcc
      601 cggcatttgc cgatgacgag tagtccgttc atcgttggtg caggcacgaa taccccgttg
      661 ttctcatgat caacaggaaa accacagaaa aagttaagaa aactctgaat cccgcaggtc
      721 aagcgaatgg cgttaattca ctcccacgtc gataattcgc ccgcttgccg cacgtcagtc
      781 gcccaattcg atgcgagccg ggtcgaacga gttgttcgct gcgcgatgcg acgaaacttg
      841 cgtatgcaac gttcagccgg ccatgcggcg gtatgtcacg tcgaagtctg tcgaccagtt
      901 ctcgccatcg tcggaggctt cccaccgtcc gtcgatcacg gtgcggccgt cgtcgaaggc
      961 gccctcgaag cgctggaaga agtcgcgacc gggccgggat tccagcgtcc agcgaccggc
     1021 gtccaggtgc attcgatagg tgcggcagac gccgcgggag tcggtataca gcatggtgaa
     1081 aacgtcggag tggtcgtcga ggccgatgac cgcgtccacc catcggggtg ccgcgtcaac
     1141 ccattccggc acaaggtagg tgggcgggtc gtttcgctga acgaggaagc cacgatcgcc
     1201 gagccagtgg aaggtcgacc gcgacctgct catgatccgt ccgcctgagg tggccgtgag
     1261 ctcccagtcc cctacgagga gatccagtcg ggtcagccgc ggattgagtg atcggtccat
     1321 gtatacaagg actgacgagc gggcgacacc tcatcgcagg cgtggtcacc gacaagctca
     1381 tcagccgttc gtcagccgct agattgatcg gaccatgaca acagacgttg ggcacgcccc
     1441 gtttctgctg ctgtcgatcc gcgccgagga tgcagccgcg gacgatgaat atgcggcgat
     1501 gatgcgcttc gctgggctgg acacgaatgg gatgcgccgg atccggctga cgcacgagcc
     1561 gttgggcgac atcgatctgg acgactggtc cggcatcatc ttgggaggcg ggccgtacaa
     1621 cgtcagcgat ccggcggatt cgaaatcgca gacgcagcga cgggtggaat ccgaactgct
     1681 gccgctgatc ggtcgcatcg tcgacgacga cttcccgttt ctcggatgct gttacggcgt
     1741 aggcacgctc ggttcggtcg tcggtgctag cgtcgaccgc acctacaccg agcctgtcgg
     1801 tgcgctgacc gtcacactca ctgaagacgg tcgggcggat ccgctattcg ccgaactacc
     1861 cgatgccttc gacgcatacg gtggccacaa ggaaggcgcc agctcgctgc cctcggatct
     1921 ggcttgcctt gcgtattcgg ccgactgccc cattcaggca tttcgcgtgg gcgacaacgt
     1981 ctatgcgacg cagtttcacc ccgaactcga cgctggcggg atctgcacgc ggatcgacgt
     2041 ctacaaggac tatggttact ttgaacccga gacggcggac tcattgaaat ctgctgcgct
     2101 acaacggaac atcgagcatc ccccgacgat cttgcggagg tttcgcgagc gctacgcccg
     2161 accggtgtga cggcgacgag cgtacgacgg cggatacgac tgcggcgcag cggttctcgc
     2221 tctttggtca gctgtcgcga gacacgccgg attcgctcac gtctttagaa atctcctacg
     2281 gtgacgaccg gttgggggtc ggcgcgaagg ttgtcgacga gcttgcggta cacctggcca
     2341 cagcgaaacc cgatgcgagc gacgtcggtc gatggccgcc aataaagata gtccagaaga
     2401 cttgactcat tccagaaaag gggcaagatg ggtggcgtga ccccggcacc ggattacgcc
     2461 gaccagctgc gtgcagctga cctgcgcgtg acacggcctc gacttgcggt gctggaagcg
     2521 gtccattccc atccgcatgc cgacaccgag acgattttcg gcgcggtgcg atccgatctt
     2581 cccgatgtgt ctcggcaggc tgtgtatgac gtgcttgccg cgctgactgg cgtgggtctg
     2641 gtccgcaaga ttcagccatc cggctccgtc gcccgttacg agtcgcgggt cggtgacaac
     2701 caccaccacg tggtctgccg gtcctgcggg gtcatcgccg acgtcgactg tgccgtcggt
     2761 gacgcgccct gcctgacgcc gtcggagcat gacgcccttg atggcttcgt gctcgacgag
     2821 gccgaggtta tctactgggg tctgtgcccc gattgttcgg cagcacaagc ttcacgatca
     2881 catccgtgat cacagcccat ttcacccaca cgaagtaagg aaggacgacg tgtccgagag
     2941 cgaaaatccg gcaatctccg caccgactgc caagtcagga cggccgcata ccaaccaaga
     3001 ctggtggccc gaccaggtcg acctttcggt tctgcacaag cacaacccgc aggccactcc
     3061 actgggcgat gactacaact acgcggaaga gttcaagaaa ctcgatgtcg aggcgttcaa
     3121 gcgcgacgtc atcgaggtgc tgaccacctc gcaggactgg tggcccgccg acttcgggca
     3181 ttacggaccg ctgatgatcc ggctgagctg gcacgccgcg ggcacctacc gcatcgagga
     3241 tggccgcggt ggcggcggcg agggcgccca gcgtttcgcc ccgctgaaca gctggccgga
     3301 caacgccagc ctcgacaagg cgcgccgcct gttgtggccc gtcaagcaga agtacggccg
     3361 caagatctcc tgggccgatc tcctgatctt cgccggcaac gtcgcctacg aatcgatggg
     3421 gttcaagacg ttcggcttcg
//
LOCUS       DASRJA010000127         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1869117,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000127 DASRJA010000000
VERSION     DASRJA010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869117"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VFC01_05715"
     CDS             <1..443
                     /locus_tag="VFC01_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZN79176.1"
                     /translation="YRADYTTRGWAMNRLFDGIDTLLADLRATGVRLAVATSKAEPTA
                     RRILAHFGLDGHFEVIAGASVDGSRATKSDVVAHALAQLRPLPDRVLMVGDRSHDVEG
                     AAEHGIDTIVVGWGYGRHDFADPHGMSALAHVFTIEDLREVLGV"
     gene            436..930
                     /locus_tag="VFC01_05720"
     CDS             436..930
                     /locus_tag="VFC01_05720"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HZN79177.1"
                     /translation="MSEPLHITFICSGNICRSPMAEKMFAHQIKERGLADAVRVSSAG
                     TGGWHAGDPADRRASHVLREHGYPTTHCAAQIADEHLSADLVVALSRNHVRMLRELGV
                     PPTKIRMLRSFDPRSAAHALDVEDPYYGTHDDFEDVFAVIEASLPGLHDWVDEQLASG
                     GVAS"
     gene            930..1748
                     /locus_tag="VFC01_05725"
     CDS             930..1748
                     /locus_tag="VFC01_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HZN79178.1"
                     /translation="MRRWAFLLRPAWLALFVVVIAFAYLCFTVLAPWQLGKNTKTSRE
                     NTQISQSLNADPVPLTTFLPHQDSSAPDDQWRRVTATGRYLPDGQVLARLRVIDGDPA
                     FEVLTPFAVDGGPVVLVDRGYVRPVQGSKVPTIAPAPTETVTITGRLRDSEPVVQDKD
                     PFRQDGAQQVYSINTAQISALTRVPLAGSYVQLVEDQPGGLGVIALPRLDAGPFLSYG
                     IQWIAFGIAAPILLGYFLYAEIRQRRREKAAAPPTPAAPLTVEQKLADRYGRRR"
     gene            complement(1702..>2314)
                     /locus_tag="VFC01_05730"
     CDS             complement(1702..>2314)
                     /locus_tag="VFC01_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CobD/CbiB family cobalamin biosynthesis protein"
                     /protein_id="HZN79179.1"
                     /translation="ARRLLPSLCGRDPAALDSAGLTRAALESVAENTSDAHVAPVLWA
                     AVGGVPAVLVYRGTNTLDAMIGNKSPRCARFGWAAARFDDAANFVAARVTALLVATCA
                     PVVGGSPAEALRAWRRDAARHPSPNAGVVEAAFAGALGVRLGGPTQYAHELEIRPTLG
                     DGRVPEISDLARAVRLSRVVQAAAVTVCLSVVGRIGRRVSARP"
BASE COUNT          374 a          828 c          761 g          351 t
ORIGIN      
        1 cctatcgtgc ggactacacc acccgcggct gggcgatgaa caggctcttc gacggcatcg
       61 acacgctgct cgccgacctg cgagccaccg gggtgcggtt ggcggtggcc acgtcgaagg
      121 ccgagccgac ggcgcggcgg atcctcgcgc atttcggact cgacgggcat ttcgaagtca
      181 tcgccggcgc tagcgtcgac ggctcacggg ccaccaagtc cgacgtcgtc gcgcatgcgc
      241 tggcccaact gcgcccgctg cccgaccgtg tgctgatggt cggcgaccgg tcgcacgacg
      301 tcgaaggcgc cgccgaacat ggcatcgaca ccatcgtggt cggctgggga tacggacgcc
      361 acgacttcgc cgacccgcac gggatgagcg cgctggcgca cgtcttcact atcgaggacc
      421 ttcgcgaggt gctcggtgtc tgagcctctg cacatcacct tcatttgctc tggcaacatc
      481 tgccggtcgc cgatggccga aaagatgttc gcccaccaga tcaaggagcg tggcctggcc
      541 gacgcggtgc gagtcagcag cgccgggacc gggggctggc acgcgggcga tccagccgac
      601 cggcgggcct cgcatgtgct gcgcgaacac ggctacccca ccacgcactg cgccgcccag
      661 atcgctgacg agcacctctc ggccgaccta gtcgtcgccc ttagccgcaa ccacgtacgg
      721 atgctgcgcg aattgggggt tccgccaacc aagatccgca tgttgcggtc gttcgatccg
      781 cggtccgcag cgcacgccct cgacgtcgaa gacccgtact acggcaccca cgacgatttc
      841 gaggacgtgt tcgccgtgat cgaggcctcc ctgcccggtc tgcacgactg ggtggacgaa
      901 cagctggcca gcgggggagt cgcgagctga tgcgccgctg ggcctttctg ctgcggcctg
      961 cgtggctggc cctgttcgtc gtggtgatcg cgttcgccta cctgtgcttc accgtgctgg
     1021 ccccctggca gctgggcaag aacaccaaga cctcgcggga gaacacccag atctcgcagt
     1081 cgctcaacgc cgacccggtg ccgctgacaa cctttctgcc acaccaagat tcgtcggcac
     1141 cggatgatca gtggcggcgg gtgacggcga ccggccggta cctgcccgat ggtcaggtgc
     1201 tggcacggct gcgcgtcatc gacggtgatc ccgcgttcga ggtgctgacg ccgttcgccg
     1261 tcgacggcgg acccgtggtg ctggtcgacc gcggatacgt gcggcccgtg cagggctcga
     1321 aggtgccgac gattgcgccc gcaccgaccg aaacagtgac gatcaccggg cggctgcgcg
     1381 actccgaacc ggttgtgcag gacaaggatc cattccgcca ggacggcgcg caacaggtgt
     1441 attcgatcaa caccgcgcag atatcggcgc tgacccgcgt cccgctggcc ggatcgtatg
     1501 tgcaactggt cgaggatcag cccggcggcc tcggcgtcat cgccctaccc cgcctcgacg
     1561 cgggtccgtt cctgtcgtac ggcatccagt ggatcgcgtt cggcatcgcg gccccgattc
     1621 tgttgggcta cttcctgtat gccgaaattc ggcagcgccg acgagagaaa gccgccgcac
     1681 cacccactcc tgccgcgcca ctcacggtcg agcagaaact cgccgaccga tacggccgac
     1741 gacgctgagg cagaccgtga cggccgcggc ctgtacgaca cgcgacaatc gcaccgcccg
     1801 agcgagatcc gagatctcgg ggactcggcc gtcaccgaga gtcggtctta tctcgagttc
     1861 atgcgcgtac tgtgtcggcc cgccgagccg cacgcccagc gctccggcga acgccgcctc
     1921 caccacaccc gcgttggggc tgggatgacg ggccgcgtcg cggcgccacg cacgtaacgc
     1981 ttcggcgggc gatccgccga cgacaggcgc gcacgtcgcc accagcagcg ctgtcacgcg
     2041 agccgcgacg aagttggcgg cgtcatcgaa acgcgctgcg gcccagccga accgggcgca
     2101 acgcggcgac ttgttgccga tcatcgcgtc cagcgtgttg gtgccgcggt acaccagcac
     2161 cgcgggcacc ccaccgaccg ctgcccacag cacgggcgcg acgtgcgcat cggaggtgtt
     2221 ctcggcgacc gattcgagcg ccgcccgcgt cagcccggcc gaatcgagcg cggccgggtc
     2281 acgtccgcac agcgacggca acagccgccg tgcg
//
LOCUS       DASRJA010000128        31607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3563033,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000128 DASRJA010000000
VERSION     DASRJA010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 31607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31607
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3563033"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1775)
                     /locus_tag="VFC01_05735"
     CDS             complement(144..1775)
                     /locus_tag="VFC01_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HZN79180.1"
                     /translation="MSARRLDASALRDWAHTAVGDLISHTDEINRLNVFPVADADTGT
                     NMLFTMRSAWAHADSESDSDDVTQVAAALAAGALHGARGNSGVILSQILRGLADVVSS
                     AAADRGEALADIDGPVFAAALRHAVGLVVASMGEPVPGTIVSVLQAAAGAAEHASADS
                     AELAAAVSAAADAATVALDKTTGQLDVLAEAGVVDAGGRGLLVLLDAMSTTLTGRAPH
                     RQEYLPSPPQASSATTAAAPQFEVMYLLSGCDARGVETLRQRLDQLGESVAIAASADG
                     AGHYSVHVHADDAGAAVEAGLSVGTASRIQITALSGSASGRPAGGWSHERAVLAVVDG
                     AGAAELFAGEGAHVLRPEDDAPVSAKQLLHALVNTGAAQIMVLPNGYLAAEELVAGCT
                     AAIGWGIDVVPVPAGSMVQGLAALAVHDAERLAVDDGYTMARAAAGARHGSVRMATEE
                     ALTWAGTCKPGDGLAIAGDEVLIVADDITAAAAGLIDLFLAAGGELITVLTGAGVDRR
                     VGEALQEHAHRHHPGTELVTYHTDHRGDALLIGVE"
     gene            1963..2157
                     /gene="rpmB"
                     /locus_tag="VFC01_05740"
     CDS             1963..2157
                     /gene="rpmB"
                     /locus_tag="VFC01_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009030042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZN79181.1"
                     /translation="MAAVCEICGKGPGFGKSVSHSHRRTSRRWDPNIQTVRAVTHPGG
                     NKKRINVCTSCIKAGKVSRG"
     gene            complement(2603..3286)
                     /locus_tag="VFC01_05745"
     CDS             complement(2603..3286)
                     /locus_tag="VFC01_05745"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZN79182.1"
                     /translation="MTARPLHELVEAGWATALEPVGAQVTQMGEFLRAEIAAGHRYLP
                     AGPNVLRAFTFPFDQVRVLIVGQDPYPTPGHAVGLSFSVAADVRPLPRSLENIFAEYG
                     KDLGFPPPTTGDLSPWAERGVMLLNRVLTVRPGNPASHRGRGWEAVTECAIRALVARQ
                     QPLVAVLWGRDASTLKPMLDGSDCVAIESPHPSPLSASRGFFGSRPFSRANELLEKMG
                     ADPIDWRLP"
     gene            complement(3315..4268)
                     /locus_tag="VFC01_05750"
     CDS             complement(3315..4268)
                     /locus_tag="VFC01_05750"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZN79183.1"
                     /translation="MASDEAETLAGVGEFAVIDRLVAGRNQPATVALGPGDDAAVVVT
                     GDGRTVVSTDMLVAGRHFRLDWSTPHDVGRKAIAQNAADIEAMGAKATAFVVAFGAPG
                     DTPTSQALELADGMWHEARLLGAGIAGGDLVSAPQWVISVTVLGDLGGREPVLRSGAK
                     SGDTVAIAGELGRSAAGYALWHSDIRQFETLRRRHLVPEPPYGQGRAAADAGATAMTD
                     VSDGLMADLGHLATASGIGIDVSTAALSADRDAVADAAAVIGGDAWAWVLGGGEDHAL
                     VATFPGTPPTGWRPIGRVVDGPARVLVDGDTWRGNPGWQSF"
     gene            4344..4586
                     /locus_tag="VFC01_05755"
     CDS             4344..4586
                     /locus_tag="VFC01_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HZN79184.1"
                     /translation="MVEAFMLIQTEVGRAEVVAKQLAALPGVLSSEYVTGPYDVVVRI
                     SADSLDQLQADVVPSVQQVTGITRTLTCPIANGVRP"
     gene            4597..5130
                     /locus_tag="VFC01_05760"
     CDS             4597..5130
                     /locus_tag="VFC01_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3515 domain-containing protein"
                     /protein_id="HZN79185.1"
                     /translation="MDAENRDGPPRALLIAAIVVAVGAVIGILGIAVLRQSPPGPQTV
                     AIASVPAPKADSAECRTLLDALPDRLGDYRRTPAAKPAPPGAAAWAAAGEPVILRCGL
                     DRPAEFVVGSPLQVVDAVQWFELADQGRSTWFAVDRPVYVALTLPQGSGPTAIQEISG
                     VIAKSLPAKPIDPAPAR"
     gene            complement(5078..7924)
                     /locus_tag="VFC01_05765"
                     /pseudo
     CDS             complement(5078..7924)
                     /locus_tag="VFC01_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RND family transporter"
     gene            8057..9265
                     /locus_tag="VFC01_05770"
     CDS             8057..9265
                     /locus_tag="VFC01_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZN79186.1"
                     /translation="MLGAVSRYVQERVRNRGEQIPIGYNWTFVITVDITLLGIFAVAV
                     LQRPTADLPIGLLALGLAVIPFALFFVLGINWEAHLVGIAWTAATAILLFATSTPIHT
                     DFAPLVLALMVGEVGSMTPRFGGFLAAASATALLLAASTLHRLDAVALYVGIVGMSWL
                     VGYLMRTQQQLMLAQQEAQTQLAEHAAADERRRIAREVHDVIAHSLSITLLHITGARR
                     GLQQDRDVDDAVEALEQAEHLGRQAMTDIRRTVGLLDGAPMKMAPEPTVGDISSLVDD
                     FVRAGMNVTLDVKGRRERVSAAVGLALYRITQESLANIAKHAPDSQSAVTLTISRHST
                     TLTVINQLPVAVSASTASEGRGVRGMRQRVHLLGGMIDIGPSDHGWSVRAEIPLTDAD
                     PTHWPPWCPV"
     gene            9274..9924
                     /locus_tag="VFC01_05775"
     CDS             9274..9924
                     /locus_tag="VFC01_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN79187.1"
                     /translation="MADVAVLLVDDQDLVRSGLRRILRRKDGFTIVAECADGDEVPDA
                     VAAHRPDVVVMDLRMKRVDGIEATGRLAAGDGPPVLALTTFNDDELLSGALRAGAAGF
                     VLKDSSAEELIRAVRAVARGDSYLDPAVTARVLTTYRKAAEPPRKATISDLTARELDV
                     LTLIAKGSSNAEIADALGISNVTVKSHIGRIFTKLDLRDRAAAIVYAYDRGIVAPR"
     gene            complement(10215..11336)
                     /locus_tag="VFC01_05780"
     CDS             complement(10215..11336)
                     /locus_tag="VFC01_05780"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HZN79188.1"
                     /translation="MTAHQPGATGRVRVAVVYGGRSSEHAISCVSAGSILRNLDPERF
                     EVVAVGITPGGSWVLTDGRPETLAITDGRLPQVSGESGTELALPADPIRGGQLLSLGQ
                     GGEMLAAVDVVFPVLHGPYGEDGTIQGLLELAGVPYVGAGVLASAAGMDKEFTKKLLV
                     AEGLPVGDHVVLRPRHDTVELEDRERLGLPVFVKPARGGSSIGVSRVTAWDQLPAAIE
                     LARRHDPKVIVEAAVPGRELECGVLEFPDGRVEASTVGEIRVAGVRGREDAFYDFETK
                     YLDDGAELDVPAKIDDELADTVRQLAIRAFRAVDCQGLARVDFFLTDDGPVVNEINTM
                     PGFTTISMYPRMWAASGVDYPTLLATMVETALARGTGLR"
     gene            complement(11477..12580)
                     /locus_tag="VFC01_05785"
     CDS             complement(11477..12580)
                     /locus_tag="VFC01_05785"
                     /EC_number="4.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-lyase"
                     /protein_id="HZN79189.1"
                     /translation="MDGTYGDSTRSVKAVGSEAIPGSPVSQPPVPASTYYLSPDETEP
                     LDSYGRSSNPTWRQLESALAELEGAATALTFGSGMAAITSALRVMAKPDGKLVVPADG
                     YYQVRRYAAEYLAPQGVTVVEARSAEMCEASADANVVLAETPVNPGLDVVDLHHLAMT
                     CRGRGAILIVDNTTATPLGQQPLSLGADLVVASATKALSGHSDLVAGYVAGSQPELME
                     ALERDRLLAGPILGAFEAWLVLRSLGSAGLRFERQCQNAQALAVMLRAHPAVKSVRYP
                     GLPDDPSHQIATLQMKRFGGLIAVELEDAAAVHALVERSALLIASTSFGGIHTSVDRR
                     ARWGDPVSDGFARISLGIEDTDDLIADFEQALG"
     gene            complement(12585..13565)
                     /locus_tag="VFC01_05790"
     CDS             complement(12585..13565)
                     /locus_tag="VFC01_05790"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZN79190.1"
                     /translation="MGAGAWGTALAKVLADAGNNVTLWARRPELADEINDTRRNTHYL
                     DDTELPKSIRATSDPAEALSGACTVLLAVPSQTMRANLEHWTHLIGDDVTLVSLAKGI
                     ELDTLMRMSQVIVQVTGADPARVAVVTGPNLASEIADEQPAATVVACSDSGRAVALQR
                     ALSTGYFRPYTNADVIGAEVGGACKNVIALACGMAAGVGLGENTAAAIITRGLAEIMR
                     LGIALGAKPATMAGLAGVGDLVATCTSPHSRNRSFGERLGKGDTMEAALLAAGGHIAE
                     GVTSCESVLALAASYDVEMPLTDAVQRVCHKGLSVDQAVALLLGRSTKPE"
     gene            13619..14281
                     /gene="cofC"
                     /locus_tag="VFC01_05795"
     CDS             13619..14281
                     /gene="cofC"
                     /locus_tag="VFC01_05795"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HZN79191.1"
                     /translation="MALMSGTRTGEAADIGLVIAVKRLTAAKTRLAPVFSAPTRESVV
                     LAMLVDTITAASAVAALQSVTVVTPDEVAADATRRLGARVLIDPTPEGHRNPLNNALA
                     AAETAILEETPNIVALQGDLPALQPQELAEAITAARGYSRSFVGDRHGTGTSALFAFG
                     VGLDPHFGPDSAQRHRQSGAIELTGAWPGLRCDIDTPDDLLVARRLGVGAATAQAIGH
                     HR"
     gene            14348..16501
                     /locus_tag="VFC01_05800"
     CDS             14348..16501
                     /locus_tag="VFC01_05800"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HZN79192.1"
                     /translation="MTEAEAQIRPDGAELVDSAPEAPPAATSPAVDNALPEDRYLNRE
                     LSWLDFNARVLALAADTSLPLLERAKFLAIFASNLDEFYMVRVAGLKRRDEMGLSVRS
                     ADGLSPREQLRRINELTQKIADRHAHVFLDSVRPALADEGIVIVTWAELDDDERSRLS
                     TYFHEQVFPVLTPLAVDPAHPFPFVSGLSLNLAITVKHPDDGGQHFARVKVPDNVDRF
                     VELARREDSEDVVRFLPMEELIAAFLSALFPGLEVVEHHAFRITRNADFEVEEDRDED
                     LLQALERELARRRFGSPVRLEVSDDMTESMLELLLRELDVYPGDVIEVPGLLDLSSLW
                     QIYGVDRPALKDPPFVPATPPAFGERETPKSIFSTLRDGDVLVHHPYDSFSTTVQRFI
                     EQAAADPNVLAIKQTLYRTSGDSPIANALIDAAAAGKQVVALVEIKARFDEQANIKWA
                     RALERAGVHVVYGLIGLKTHCKTCLVVRREGSTIRRYCHIGTGNYNPKTARLYEDVGL
                     LTAAPDIGADLTDLFNSLTGYSRKDSYRNLLVAPYGVRRGIIERIEREIAATRDGAEG
                     QIRLKANALVDEQVIDALYRASQAGVRVEVVVRGICALKPGAAGYSENIVVRSILGRF
                     LEHSRIIHFRAIDEFWIGSADMMHRNLDRRVEVMAQVKDPRLTAQLNDVFESAMDPAT
                     RCWELGVDGHWIASPQEGQTVRDHQVSLMERHRHP"
     gene            16552..17481
                     /locus_tag="VFC01_05805"
     CDS             16552..17481
                     /locus_tag="VFC01_05805"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZN79193.1"
                     /translation="MPKELPLDAVPASKAVLAAGAVLWRSNGDPSSPEIAIIHRPRYD
                     DWSLPKGKVDPGETEPVTAVREVHEETGYSSHLGRRLAAVSYPVEQGIKKVRYWAARR
                     VDGEFNPNAEVDELKWLPVAEALKQLEYPHDRKVLRRFAKQPVDTQTVLIVRHGTAGS
                     KSRYKGDDRKRPLDKHGRAQAESLVGVLLAFGADVLYAADRVRCHQTLEPLAEELGAT
                     IQGEPLLTEEAYADNRKAARHRFLEIAAAGGTPVICTQGKVIPDLIAWWSERDGVRPD
                     KSRNRKGSAWVMSLANERLIAADHIGSPLAAKK"
     gene            17541..17684
                     /locus_tag="VFC01_05810"
     CDS             17541..17684
                     /locus_tag="VFC01_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79194.1"
                     /translation="MPSGGDAYVLKQIVRDWDESKGLEILRSVPMMIASICPNCSMTM
                     PSC"
     gene            complement(17827..18426)
                     /locus_tag="VFC01_05815"
     CDS             complement(17827..18426)
                     /locus_tag="VFC01_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZN79195.1"
                     /translation="MNKAELIDVLTEKLGSDRRQATAAVENVVDTIVRAVHKGDSVTI
                     TGFGVFEQRRRAARVARNPRTGETVRVKPTSVPAFRPGAQFKAVVSGAQRLPAEGPAV
                     KRGVTAGGARKAVRKAAAKKAVKRAAVKKAVKRAAVKKAVKRAAVKKAVKRTAAKKAV
                     KRTAAKKAVKRTAAKKAVKRAGVKKAAAKRAPAKKGGRR"
     gene            complement(18631..19224)
                     /gene="leuD"
                     /locus_tag="VFC01_05820"
     CDS             complement(18631..19224)
                     /gene="leuD"
                     /locus_tag="VFC01_05820"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZN79196.1"
                     /translation="MQAFRTHTGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFA
                     AWRTDPSFILNLSPFDKGSVLVSGPDFGTGSSREHAVWALMDFGFRVVISSRFADIFR
                     GNAGKAGLLAAEISQDDVELLWKLIEEHPGMEITVNLQDRNIVAGTVMVPFNIDDYTA
                     WRLLEGLDDIGLTLRKLDAIESYESSRPNWKPRTLPV"
     gene            complement(19237..20661)
                     /gene="leuC"
                     /locus_tag="VFC01_05825"
     CDS             complement(19237..20661)
                     /gene="leuC"
                     /locus_tag="VFC01_05825"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZN79197.1"
                     /translation="MTTPRTMAEKVWADHVVAHGTGEGTAREPDLIYIDLHLIHEVTS
                     PQAFDGLRLNNRAVRRPDLTIATEDHNVPTIDIDKPIADPVSRTQVETLRRNCEEFGI
                     RLHPMGDAEQGIVHIIGPQLGLTQPGMTVVCGDSHTSTHGAFGALAMGIGTSEVEHVL
                     ATQTLPLRPFKTMAVNVDGQLPPGVSAKDIILAVIAKIGTGGGQGHVIEYRGSAIESL
                     SMEGRMTICNMSIEAGARAGMVAPDETTFEFLRGRPHAPTGADWDAAVAAWTQLRTDE
                     GAEFDTEVYLDASTLSPFVTWGTNPGQGVALSDSVPDPELMFDDAERQTAEKALAYMD
                     LRPGTAMRDIAVDAVFVGSCTNGRIEDLRVVADVLRGRKVANGVRMLIVPGSMRVRAQ
                     AESEGLGEIFTAAGAEWRQAGCSMCLGMNPDQLSPGERCASTSNRNFEGRQGKGGRTH
                     LVSPAVAAATAVRGTLASPADLTN"
     gene            20701..21402
                     /locus_tag="VFC01_05830"
     CDS             20701..21402
                     /locus_tag="VFC01_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN79198.1"
                     /translation="MRQDSGIGVLDKAVGVLHAVADSPCGLAELCERTGLPRATAHRL
                     AAGLEVHRLLSRNGDGRWRLGPALTELAGQVNDPLLAAGAVVLPRLREITGESVQLYR
                     REGTSRICVAALEPPAGLRDTVPVGTRLPMTAGSGAKVLLAYADAATQQAVLPAAMFT
                     DRALAEVRKRGWAQSAAEREPGVASVSAPVRDARGAVIAAVSVSGPIDRMGRRPGARW
                     AADLLAAADALTRRL"
     gene            complement(21445..21517)
                     /locus_tag="VFC01_05835"
     tRNA            complement(21445..21517)
                     /locus_tag="VFC01_05835"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21481..21483),aa:Glu,seq:ctc)
     gene            complement(21608..21679)
                     /locus_tag="VFC01_05840"
     tRNA            complement(21608..21679)
                     /locus_tag="VFC01_05840"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21644..21646),aa:Gln,seq:ctg)
     gene            complement(21798..23276)
                     /gene="gltX"
                     /locus_tag="VFC01_05845"
     CDS             complement(21798..23276)
                     /gene="gltX"
                     /locus_tag="VFC01_05845"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZN79199.1"
                     /translation="MTVRVRFCPSPTGTPHVGLIRTALFNWAYARHTGGAFVFRLEDT
                     DAERDSEESYLALLDALSWLGLNYDEGPDIGGPYAPYRQSERSEMYRDIVQRLLDAGE
                     AYEAFSTPGEVEARHIAAGRNPKLGYDNFDRTLTDEQRAAFVADGRTPVVRLRMPDED
                     ITWHDLVRGETTFAAGSVPDFALTRASGDPLYTLVNPVDDALMRITHVLRGEDLLPST
                     PRQIALYQALIRIGVAERVPEFAHLPSVLGDGNKKLSKRDPQSNLFLHRERGFIPEGL
                     LNYLALLGWGIAEDRDVFSLDEMVAAFDVVDVNSNPARFDQKKADALNAEHIRLLSEE
                     DFTERLRTYFATHGHDTGLDGSQFAEAARLVQTRIVVLGDAWDLLKFLNDSEFTLDEK
                     AAAKELRGDAAPVLDAAIAALEEVGEWTTAKIEIALKVALLENLALKPRKAFGPIRVA
                     ATGASISPPLFESLELLGRDRSLRRLRGGRGYATAAAAAAEA"
     gene            complement(23273..24061)
                     /locus_tag="VFC01_05850"
     CDS             complement(23273..24061)
                     /locus_tag="VFC01_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZN79200.1"
                     /translation="MRLGRIASPDGVAFVSIEGATDDPNEAIAKEIAEHPFGTPNFTG
                     RQWPLADVRLLAPILASKVVCIGKNYAAHVAEMGGGSFPDPVIFMKPNTAIIGPGVPI
                     QMPADANPVHFEGELAAVIGRPCKDVPAARVAENILGYTIGNDVTARDQQKKDGQWTR
                     AKGYDTFCPIGPWIVTDVDPADLDIRTEVNGEVKQHSRTSLMIHDIGAIVEWISAVMT
                     LLPGDLILTGTPEGVGPIEDGDTVSVTVEGIGTLSNPVVRKGKS"
     gene            24167..25372
                     /locus_tag="VFC01_05855"
     CDS             24167..25372
                     /locus_tag="VFC01_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN79201.1"
                     /translation="MSIESISSVRRWSMLVIALTATLCANVFINGAAFLIPTLHTERG
                     LDLAKAGLLSSMPSFGMVMTLIAWGYVVDRVGERTVLALGSALTAAAAFAAASVQSLV
                     AVGAFLLLGGMAAASSNSASGRLVVGWFAPERRGLVMGIRQTAQPLGVGLGALVIPRL
                     AESHGTSVALLFPAVVCAVSAAVCAVAVIDPPRPPRHEADEADLANPYRGSSVLWRIH
                     AVSVLLVVPQVVVWTFTLVWLMSDRGWSAASAGAMVTAAQILGAGGRIAAGRWSDIAG
                     SRLRPIRTIALAAAAAMGLLAVTDWLNSPLSVAVMVIASVITVSDNGLAFTAIAEIAG
                     PFWSGRALGTQNTSQLLTAGVAPPLFGGLIGIAGYPVAFAVCALFPVIAMPLVPVDSD
                     PLRQADTSR"
     gene            complement(25376..25810)
                     /locus_tag="VFC01_05860"
     CDS             complement(25376..25810)
                     /locus_tag="VFC01_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZN79202.1"
                     /translation="MAESETERAVGYLVKRVQQSLRRRSDAALRSVGVSMAQYAVLRA
                     LADHPDASASELSRLCFVTRQSLQDVLFGLRSAGLIVESDRPARGRARSLELTAAGTK
                     RLSAAHTAVMGVESAMTEGISPTTRRQLVTALTRCAENLETA"
     gene            25869..26192
                     /locus_tag="VFC01_05865"
     CDS             25869..26192
                     /locus_tag="VFC01_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HZN79203.1"
                     /translation="MTTIEQNSAYATLINVFTVEPDRAAELAALLNTATEEVMRHRPG
                     FRSANIHVSTDGSRVVNYAQWDSADAYRSMLKDPIAQRHMSEAAALAISFDPHLYTVE
                     SVHER"
     gene            26265..26558
                     /locus_tag="VFC01_05870"
     CDS             26265..26558
                     /locus_tag="VFC01_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79204.1"
                     /translation="MLASSVQRGGIDEAHSDLPARHNRRTALIEAALAPARSQFTTTA
                     LKSLTRSLALIIGTESMVVLKDVLQLDEPAARKVRRWAIRALVEAAQKPRSRN"
     gene            26580..26774
                     /locus_tag="VFC01_05875"
     CDS             26580..26774
                     /locus_tag="VFC01_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79205.1"
                     /translation="MILDGLVDACGEPILSPLLRRPGVVLSSAGIAGYGAAFAVCALF
                     PLMALPLVPVVADPLMKGRG"
     gene            26790..27296
                     /locus_tag="VFC01_05880"
     CDS             26790..27296
                     /locus_tag="VFC01_05880"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZN79206.1"
                     /translation="MTGAAIDIINDYFTCEFTTLSRDGSPHAWPVSPRLLTDGRLLLT
                     TSIGLPQKAFNVRRNPKVSLLFSEPTGSGINQPGAVLIQGDATSEDRIVTDPLSDPDF
                     AALAETVSTRQPAGNLWTTWLGRRLWWSYYMRLLIYVTPRRAFFWPTRDFTSSPEELD
                     LSEVRRVG"
     gene            27286..27723
                     /locus_tag="VFC01_05885"
     CDS             27286..27723
                     /locus_tag="VFC01_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79207.1"
                     /translation="MWAEAAKWLGKFDEAVLTALDIDGYPFSVRVDTRTYDADTGRLP
                     AALPGVLRAAESPASLLCHYHDEKMWKLKAIQVKGRLEKRNDAWDFVTTSFTPPSKLA
                     LFAFLKGTGTAAQKYLDRRGLERPKVNWVAVNEIKRRVSEAKR"
     gene            complement(27720..28730)
                     /locus_tag="VFC01_05890"
     CDS             complement(27720..28730)
                     /locus_tag="VFC01_05890"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZN79208.1"
                     /translation="MKLAIIAGDGIGPEVVGEALKVLDAVVPGIEKTGYDLGARRYHA
                     TGEVLPDSVIDELKAHDAILLGAIGDPSVPSGVLERGLLLRIRFALDHHVNLRPSRLY
                     PGVNSPLAGNPDIDFVVVREGTEGPYTGTGGAIRVGTPHEIATEVSVNTAFGVQRVVH
                     DAFRRAQRRRKHLTLVHKNNVLTFAGALWWRTVQEIATEYPDVEIAYQHVDAATIHLV
                     TDPGRFDVIVTDNLFGDIITDLAAAVCGGIGLAASGNIDATRTNPSMFEPVHGSAPDI
                     ACQGIADPTAAIMSVALLLAHVGEDDAAARVDKAVEHHLATRGDEKLSTAQVGDRIAA
                     NL"
     gene            complement(28727..30313)
                     /gene="serA"
                     /locus_tag="VFC01_05895"
     CDS             complement(28727..30313)
                     /gene="serA"
                     /locus_tag="VFC01_05895"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZN79209.1"
                     /translation="MSLPVVLIADKLAESTVAALGDQVEVRWVDGPDREKLLAAVPEA
                     DALLVRSATTVDAEVLAAAPKLKIVARAGVGLDNVDVDAATARGVLVVNAPTSNIHSA
                     AEHALALLLSAAREIPAADASLRERTWKRSKFSGTEIYGKTVGVVGLGRIGQLVAQRL
                     AAFGTHVVAYDPYVSAARAAQLGIELLTLDELLGRADFISVHLPKTQETAGLIGKEAL
                     AKTKPGVIIVNAARGGLVDEEALAEAVRSGHVRAAGLDVFAKEPTTDSPLFDLPQVVV
                     TPHLGASTGEAQDRAGTDVAESVKLALAGEFVPDAVNVGGGVVGEEVAPWLDLVRKLG
                     LLVGVLSTEVPLSLSVQVRGELASEDVEVLRLSALRGLFSAVIEDPVTFVNAPALAAE
                     RGVQADISTASESPNHRSVVDVRAVFSDGSVTNVAGTLSGPQLVEKIVQINGRNFDLR
                     AEGINLIIHYLDKPGTLGKIGTLLGAADINIHAAQLSEDAEGPGATILLRIDRDVPAD
                     VRATITEAVGAKLLEVVDLL"
     gene            30346..>31607
                     /locus_tag="VFC01_05900"
     CDS             30346..>31607
                     /locus_tag="VFC01_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN79210.1"
                     /translation="MDVTVVGSGPNGLAAAVICARAGLSVQVFEAQPTLGGGARTLAD
                     PEFSGVAHDICSAVHPLALASPFLAEFDLPSRGVTLKVPEASYANPLPGRPAAVGYHD
                     LDRTCTELTNGESWRRLLGPLVERDDAVLKLLLGDKRSIPTDPIAAIRVARRLLEQGT
                     PAWRALSGVDARALFTGVAAHTISQMPSLVSSGAGLMLATLAHTVGWPIPVGGSQAIP
                     DALIADLQAHGGTLTADEEITEPPSGVVLFDTAPTALLSIYRDRMPIRYAKALQRYRF
                     GPGVAKVDFVLSDEIPWKDSRLAGAPTLHMGGTREQMAHAEREAAAGRHAEWPMVLAA
                     LPHLADPKRIDAQGRRPLWTYAHVPSGSPVDQSETITKIFERFAPGFRDVVVAARSVP
                     ASRLAEHNANLVGGDIGVGGNTLFHALAG"
BASE COUNT         5359 a        10602 c        10246 g         5400 t
ORIGIN      
        1 gagcactgtc atggcgtcgc tgtacttgcg cgggtaatgg cgcagcaggt cgttgacggt
       61 gcggaagccg aagtgttcct cgagcaggcc cgcggccttc ttgccgagga cgtagtccag
      121 tcggtcggtc agcactgcca cagctattcc accccgatca gcagcgcgtc cccgcggtgg
      181 tcggtgtggt acgtcaccag ctcggtgccg gggtggtggc ggtgagcgtg ctcctgcagc
      241 gcttcgccga cgcggcggtc gacacccgca ccggtgagca cggtgatgag ctcgccacct
      301 gcggcgagga acaggtcgat cagccccgcc gcagccgcgg tgatgtcatc ggccacgatc
      361 aacacctcgt cacccgcgat ggccaacccg tcgccgggtt tacaggtgcc cgcccatgtc
      421 agcgcctcct cggtggccat ccgcaccgag ccgtgccggg cgccggcggc cgcgcgggcc
      481 atggtgtagc cgtcgtcgac ggctaggcgc tcggcgtcgt gcaccgccaa cgcggccaag
      541 ccctgaacca tggatccggc gggcacaggc acgacatcga tgccccaacc gatcgccgcc
      601 gtgcatccgg ccacgagttc ttcggcggcc aggtagccgt tgggcaacac catgatctgc
      661 gccgcacccg tgttgacgag tgcgtgcaac agctgcttgg cgctcacggg tgcgtcgtct
      721 tctggccgca acacgtgcgc accctcaccg gcgaacagtt ctgcggcgcc ggcaccgtcg
      781 accaccgcca ggacggcgcg ttcatggctc cacccacctg ccggccggcc actggcgctg
      841 cccgacaacg cggtgatctg gatccggctg gccgtgccga ccgacaagcc cgcctcgacg
      901 gccgcgcccg catcgtcggc atgcacgtgt accgagtagt ggcctgctcc gtcggccgac
      961 gcggcgatgg ccaccgattc gcccagctga tccagccgtt gacggagcgt ctcgacgccg
     1021 cgggcgtcgc aaccgctgag caggtacatg acctcgaact gcggcgccgc ggcggtggtc
     1081 gcgctggagg cctgcggcgg cgacggtagg tactcctgcc ggtgcggtgc ccgtcccgtc
     1141 agcgtggtgc tcatcgcgtc cagcagtacc agcagacctc ggcctccggc gtccaccacg
     1201 ccggcctcgg ccagcacgtc gagttgcccg gtggtcttgt cgagcgcgac cgtcgcggcg
     1261 tcggcggccg cggacaccgc agcggcaagc tcggcgctat cggcgctcgc atgttcggcc
     1321 gcacccgccg cggcctgcag caccgagacg atggtgccgg ggacgggctc gcccatcgac
     1381 gcgacgacca gaccgaccgc gtgccgcagc gccgcggcga aaacgggacc gtcgatgtcg
     1441 gccagcgctt cgccgcggtc cgcggcggca gacgagacga catcggccaa gccacgcagg
     1501 atctgcgaca ggatgactcc ggagttgcca cgcgcgccgt gcagcgcgcc ggcggccagc
     1561 gcggcagcga cctgcgtgac gtcgtcgctg tcggattcgg agtcggcgtg tgcccacgcc
     1621 gaacgcatcg tgaacagcat gtttgttccg gtatcggcgt cggcaaccgg gaacacgttg
     1681 agccgattga tctcgtcggt gtgcgaaata aggtcgccga cggcggtgtg tgcccagtcc
     1741 cgcagggcgg aggcgtcgag ccgccgagcc gacatcgaaa cctcccaccc gatatgccgc
     1801 cgtcggcgcc agcctagcca cagtccctga caggacccgt cacaacgttc gggccggtgg
     1861 atgcgatttt ggcgatacct tccacggccg gtatcctggt caggttgtcg ggtcacccgc
     1921 ctggttgttg gcccccaagt actgatccga ggagttccac atatggctgc cgtctgcgag
     1981 atctgcggga agggccccgg cttcggcaag tcggtgtcgc actcccatcg ccggaccagc
     2041 cgtcggtggg atcccaacat tcagaccgtg cgcgccgtga cccaccccgg tgggaacaag
     2101 aagcgcatca acgtgtgcac gtcctgcatc aaggccggca aggtctcgcg cggttaactg
     2161 cgtttagctg tcgtttcgcc gggtaactac cggcgacatg actactcgca gcgtcctctc
     2221 gaattctgcc ctgttcgcgc tggccgccgg tgttgcggcg ggttcgctgg cgaccggctg
     2281 tggtggcgac gattccacta aggaaacgac gaccactaca acgaccacga ccacgaccac
     2341 aacgactgaa gccaccactt ccgcccccac gacgacagtg acggtctcgc cggcggtccc
     2401 ggcgccagcc cccggtgagg gagacacaac cgtcgtcgtg cctggcgtcc catccccacc
     2461 ggctatcccg tcgccgccgc cgatcccgtc cccgcccccg atcccatcac cgccgccgat
     2521 cccgggcgtg ggttagtcgt cgcgagcaga cacaaaactg cccatttcat acgaaaatga
     2581 ggcagttttg cgtctggtcg cgctaaggaa ggcgccagtc gatcgggtcg gctcccatct
     2641 tctcgagcag ttcattcgcg cggctgaacg gccgcgagcc gaagaaaccg cgcgacgccg
     2701 acagcggcga gggatgcggc gattcgatcg ccacgcagtc gctgccgtcg agcatgggtt
     2761 tcaacgtgga ggcgtcacgg ccccacagca ctgccaccag cggctgctgc cgcgcgacga
     2821 gcgcccggat cgcgcattcg gtcaccgctt cccagcccct gccccggtgt gacgccggat
     2881 tgcccggccg cacggtgagc accctgttca gcagcatcac gccgcgctcg gcccacggcg
     2941 acaggtcgcc cgtggtcggc ggtggaaaac ccaggtcctt gccgtattcg gcgaagatgt
     3001 tctccaggct gcgcggtagt ggtcgcacgt cggccgccac cgagaagctc aaaccgacag
     3061 cgtgacccgg cgtcgggtag ggatcctgtc cgacgatgag cacccgtacc tgatcgaacg
     3121 gaaatgtaaa ggcacgtaac acattcgggc ccgcgggcag gtaccggtgg ccggccgcga
     3181 tctcggcccg caggaactcc cccatctgag tgacttgcgc gccgaccggc tccagcgcgg
     3241 tggcccagcc ggcttcgacg agttcgtgca gtggacgtgc ggtcacgaaa gctcaaccta
     3301 gcttgcgcgg cgggtcagaa cgactgccaa cccggatttc cgcgccacgt gtcgccatca
     3361 accaagaccc gcgcaggccc gtcgacgacc cggccgatcg ggcgccaacc ggtcggcggc
     3421 gtgccgggga acgtggcgac cagcgcgtgg tcttcgccgc cgccgaggac ccacgcccac
     3481 gcgtcgccgc caatcaccgc ggcagcgtcg gccacggcat cacggtcggc tgaaagcgcg
     3541 gcggtcgaca catcgatgcc gataccggac gccgtcgcga gatggccgag gtcggccatc
     3601 aagccgtcgg acacatcggt catcgcggtg gctcccgcat cggcggcggc gcggccctgc
     3661 ccatacggcg gctcgggcac cagatggcgc cggcgcagcg tctcgaactg gcgaatatcg
     3721 ctgtgccaca atgcatatcc ggcggcagat cggcccagct cgcccgcgat cgcaaccgtg
     3781 tcacctgact tcgcgccgct gcggagcacc ggctcgcgac cgccaaggtc gccgagcacc
     3841 gtcaccgaaa tcacccactg cggggcgctg accagatcac cgccggcgat ccccgccccg
     3901 aggagccgcg cctcgtgcca catgccgtcg gccagctcga gcgcctgcga cgtcggggtg
     3961 tccccgggcg cgccgaacgc gacgacaaac gcggtggctt tcgcccccat cgcctcgatg
     4021 tcggcggcgt tctgcgcgat ggccttgcgt cccacgtcat ggggtgtgga ccagtccagc
     4081 cggaagtgac gtccggccac cagcatgtcg gtggacacca ccgtcctacc gtcgccagtg
     4141 acgactaccg cggcatcgtc acccggcccg agcgcgaccg tcgccggctg attgcggccc
     4201 gccaccaggc gatcgatcac cgcgaactcc ccgaccccgg ccagggtctc ggcctcgtcg
     4261 ctcgccatgt cacctccccc gggctgcggt acgtttgggc cctgcggcca ggagtctatg
     4321 cagccagatg gaggggataa cgggtggtgg aggctttcat gctgatccag accgaggtgg
     4381 gccgcgccga ggtcgtcgcc aagcagctcg ccgccctgcc cggggtgttg tcgtcggagt
     4441 acgtgaccgg cccgtacgac gtggtggtcc gtatcagcgc agacagcctc gatcagctgc
     4501 aggccgacgt cgttccgagt gtgcagcagg tcaccggcat cacccgcacc ctgacctgcc
     4561 cgatcgccaa cggagtgcgc ccctagagtt tgggccgtgg acgccgagaa ccgcgacggg
     4621 cccccgcggg cattgctgat cgctgcgatc gtcgtggcgg tcggcgcggt catcggcatc
     4681 ctcgggatcg ccgtgctgcg gcagtccccg cctgggccac aaaccgtcgc gatcgcatcc
     4741 gtgcccgcac cgaaggccga cagtgcggaa tgccgcacgc tcctcgacgc gctccccgac
     4801 cgcctcggtg actatcgccg cacaccggct gccaaacctg caccgccggg ggcggcggcc
     4861 tgggcggcgg ctggtgagcc agtcattctg cggtgcggcc ttgaccgacc cgccgagttc
     4921 gtcgtcggtt ccccgctgca ggtggtcgac gccgtccagt ggttcgagct tgccgatcag
     4981 ggtcgcagca catggttcgc ggtcgaccga ccggtctatg tcgcgttgac attgccgcag
     5041 ggatccgggc cgacggcgat tcaggagatc tctggcgtca tcgcaaagtc gttgcctgcc
     5101 aagccgattg atcccgcacc ggcgcgttag aacgctctcg acgcggcgcc ttccacgttg
     5161 gccaccagaa ccaccggccc agcagggcaa tgagcgacgg cagcacgaag gtgcgcacga
     5221 cgagggtgtc gagcagcaag ccgacgccga tcgttgtccc gatctgggcg atgctgagca
     5281 cggtgctgcc ggcaagggcg aacatcgtga tgccgaaaac gattccggcg gtggtgacca
     5341 cgccgccggt ggcggcgaaa gcgcgcacga tgccggtgct caggcccgcg tcggcctctt
     5401 cgcggatgcg cacggccaat agcaggttgt agtcggctcc gacagcgacc agtgcgatga
     5461 acgagatcgg cgccaccgac cagtgcaagt catggcccaa tgcgtgctgc cagatcagca
     5521 cgctggcccc cagcgcggag gcataggaca gcgcgaccgt gccgaccacg acgacgcccg
     5581 cgacagggct gcgcagcatc agcgcgacga tgaggaagat cagcgcaagc gtgctggcca
     5641 acagcaaggt cacgtcgcgg ccgacgagcg tctgcaggtc aatgctggtc gcgccaacgc
     5701 cggcgatatg cactgcggtc ggcgtgagcg tgccctcttt ggtggcttct cgcacggccg
     5761 tctggatttc gcgcgaccgc tccgcgccgt cgccgctcca ttcgtgcgcg tctccgtaga
     5821 tcagcaggta tgttgcgcgg ccatccttcg atatcagttt gtccaaggcc gtttgcagtc
     5881 gcggatctgt caacgcccgc cgcggtagat agaagccgcc cgcggcgctg ccacgaaact
     5941 gcatctccat ctcctggagg tagtcggtca gttggcgaag ctgcggcccc aacgactgcg
     6001 ctagctgtgt cagatcacct gtagccgcgc gggcctgcct caactgatcg gccatggcat
     6061 tgacggctga cggcagcgcg gcaagcgagt tggtcgccgc ggtcgatccg tcggtgagtt
     6121 tgaccgcacc gccactgatt tgggccgagc tgcgcatcac gtcgtcgacc ggctgcacaa
     6181 cccgcgccac caccgaacag atcggattgg caggacagtc cggtgtcgac gacatgaagc
     6241 cgcgcaacgg gtcgaggtag cccgacaccg tcgtcgcatt ggcctgtacc ccggtcatgc
     6301 cggcgcgaat gtcttcggac gccgagccga tctcccttaa tccgtctgca ccgcccttca
     6361 atccgctggc aaggcgatcg atcgctgcgg ccatcgacga cagcgccgtg tcgagatctg
     6421 cgacgcgacc cagtcggtcc gtgacgctgt cgaacgcgtc attgagttga cggccgatga
     6481 cgccggcctg cccgctgaga gtggcctcat cgggtaccgt gccggccggt cggctcgccg
     6541 attgcaccat cctgacgccg ggaatggcca tgagctgccc tgtaatccgt tcgatcgcga
     6601 tcagtccggc agggttgcgg atgtcatggt cggccgcgag cgtgagtacc gttggcagca
     6661 gctggttcgg cggaaagtgt cgatcggcgg ccgcataacc gcggttggat tcggcggtag
     6721 cgggggtggc gcccggctcg ttccagccga tctgcagccc ggccaacggc agcgccgcga
     6781 cgatgatcag tgccccgctg gcggcaagga ttggtccggg ccaccccgcc accctcactc
     6841 cgatgcgccg ccagcgtcgc gtggtcagcg agcggcgagg ctcgagccag ccgcggcggc
     6901 cggtcaaggc gatcagcgcg ggcgtcagcg tcaacgatgc gagcatcgcc acgagaacac
     6961 ctatcgcgca agggattccg gtgcttcgga acaccgcgac gtcggccatg ctcaagcagg
     7021 tcaacgcaac ctgcgatcgt caaagccgat cctgcgatca ccggggccac accgcgatag
     7081 gcgccgacga gggcgtcgtg ccattcgatg ccggaccgcc ggccctcgtg acaccggccg
     7141 atcagaaaga tcgcgtaatc ggtgccggcc ccaggagcat cgcagccagc agcgcgaccg
     7201 agaacaagga cacctccacc agaccggtct caccaagtgc cgccacgatg ggccgggcca
     7261 cggcgatggc gaggccgacg gaaaacagcg ggatcgccgc gccaacgggc gagcgataca
     7321 caatcaacaa gagcagaagg atcagtccga ctgtcgctgc ggtgataccc agcatctgcc
     7381 ggtcgatcgc ggtgaattcg tcggcgatcg tcgctcctgg acccgtgacg tacacccgca
     7441 gaccccgggg tggaccgagg cgggccacgg tgtcacgaac cgcgccaacc gaatccgtgg
     7501 cctgcgaggt gccgatcatc cccgaaagcc gcatcatgac ggtcacggcg cgacggtccg
     7561 cgctttgtcc cgcagccgcg gtggccgggt cggaccacag gtctgtcacc gtggtcacgt
     7621 gtgcggtgtc ggcgcgcaac gtctcgatca gttcggcata gaaccggcga tcctgctcgc
     7681 tcagtggctg atcacgttcc agcacaagaa aattgaagtt attgctgcgc gcctcgccga
     7741 ataggtcggc cgccgccgaa gcggccacag aacttgcggc gtcggcgggc agaaaggacc
     7801 gcgaatgact ttcaacgaca cgttcgagct gcggcaccgc catattcgcg gcacccgcgc
     7861 cgaccaacca gacgacaacg atccagatgg cataacggct cgtgaaccgt gccaacgtgt
     7921 ccataagtcg acgctaggag acctgcatct tcacggcgtc gtgccgaagt ccgaatctcg
     7981 atttgcgacc gtggtaggac gcggatgtcg accagagcat gatgtggccg gcgccagatc
     8041 cttctaggat gacacgatgc tgggtgcggt gtcgcgctac gtgcaggagc gagttcgcaa
     8101 caggggcgag cagatcccga tcggctacaa ctggacgttc gtcatcaccg tcgacatcac
     8161 tttgttagga atcttcgctg tcgcggtgct gcagcggccc accgcagact tgcccattgg
     8221 gctgctcgcg ctcggcctag ccgtcatacc ctttgcgctg ttcttcgtcc tgggcatcaa
     8281 ctgggaagcg catttggtcg ggatcgcctg gacggcggcc accgcgatcc tgctgttcgc
     8341 cacctcaacg cctattcaca ccgacttcgc gccactggtg ctggcgctca tggtgggcga
     8401 ggtcggatcg atgacgccga gattcggcgg cttcctggct gccgcgtcgg ccaccgctct
     8461 tctgctcgcc gcgtccacgc tgcatcgact cgacgccgtc gcactgtacg tcgggatcgt
     8521 cggaatgtca tggctggtgg gctatctcat gcgtactcag cagcagctga tgctcgcgca
     8581 acaggaagcg cagacacaac tggccgagca cgccgccgcc gacgaacgga gacgcatcgc
     8641 gcgcgaagta cacgacgtga tcgcccattc cctcagcatc acccttcttc acatcactgg
     8701 tgcacggcgc gggctgcaac aggaccgcga cgtcgatgac gcggtcgagg cactcgaaca
     8761 ggccgagcat cttggcaggc aggcgatgac cgacatccgc cgcaccgtcg ggctgctcga
     8821 cggtgctccg atgaagatgg ctcctgagcc aaccgtcggc gacatcagca gtctggtcga
     8881 cgatttcgtc cgcgccggaa tgaatgtcac gctggacgtc aaggggcggc gtgaacgcgt
     8941 gtcggctgca gtcgggttgg cgctgtaccg catcacccag gagtcgctgg ccaacatcgc
     9001 caagcacgct cccgattccc aatccgcggt gacgctgacc atctcccgcc attcaacgac
     9061 gctgaccgtc atcaatcagc tgccagtcgc cgtgtccgcc tcgacggcat cggaaggccg
     9121 tggcgtgcgc ggcatgcggc aacgagttca cttgctcggc gggatgatcg acatcggccc
     9181 gtcggatcac gggtggtcgg tgcgcgcgga gattcctctg accgacgccg accccacgca
     9241 ctggccgccg tggtgccccg tgtgactggc gaaatagccg acgtcgcggt gctgctcgtc
     9301 gacgaccagg acctggtgcg ctccgggctg cgtcggatcc tgcgccgcaa ggacggcttc
     9361 acgatcgtcg ccgaatgcgc tgacggtgac gaagtacccg atgccgtcgc tgcgcatcgg
     9421 ccggacgtcg tcgtgatgga cctgcgcatg aaacgtgtcg acggcatcga agcgaccggt
     9481 cggctggcgg ccggcgatgg gcctccggtg ctggcgctca ccaccttcaa tgacgacgag
     9541 ttgctctctg gcgccttgcg cgccggtgct gcggggttcg tgctcaagga ttcctcggcg
     9601 gaggaactga tccgcgctgt gcgggcggtg gccaggggcg acagctacct ggatcccgcg
     9661 gtcacagcgc gagtgctgac cacttatcgc aaggcagccg agccccctcg caaagcgacg
     9721 atcagcgacc tcacggcgcg tgagctcgac gtgctgacac tgatcgcaaa gggctcgtcc
     9781 aacgccgaaa tcgccgatgc ccttggcatc tcgaatgtca ccgtcaagag ccacattggc
     9841 cgcatcttca ccaagctcga cctacgcgac cgcgcggccg cgatcgtgta cgcatacgac
     9901 cgcgggatcg ttgcgccccg ctaacgctgt tcagagtctg aacatttcgc gtgccatcgc
     9961 ggcgtcctcg atgaacggtt cgcggtgcgc aatcgagggt ggtggctttg gtcgcgatgg
    10021 acgacgtacc acgtgggtta cgaggtgagt cagctcgatc agccagcgcc gaatgctggt
    10081 gtgcggtacc ggcgtagcga ttgcccatgt catgagttga cgttaggaaa tcggcatcca
    10141 gatgcgcatc gtgccagggt ccggtctttt ttctcctacg gtggtaggag ctgccgccgc
    10201 ctagggcagg aggtctagcg cagcccggtg ccgcgcgcga gcgccgtctc gaccatggtg
    10261 gccaacagcg tcggatagtc cacgccgctg gccgcccaca tccgtggata catcgatatc
    10321 gtggtgaagc ccggcatggt gttgatttcg ttgactaccg gcccgtcgtc agtcaggaag
    10381 aagtcgacgc gagccaggcc ctggcagtcc acggcgcgga aggcccgaat ggctaactgc
    10441 cgcactgtat ctgcgagttc gtcgtcgatc ttggctggca cgtcgagctc tgcgccgtcg
    10501 tcgagatact tcgtctcgaa atcatagaac gcatcctcgc gcccgcgcac cccggcgacc
    10561 cggatctcgc cgaccgtgct ggcctcgacc cgcccatcgg gaaattccag gacgccacac
    10621 tctaattcgc ggccgggcac ggccgcctcg acgatcacct tgggatcgtg cctgcgtgcc
    10681 aattcgatcg ccgcgggcag ctggtcccat gcggtgaccc ggctgacgcc gatagacgag
    10741 ccgccgcgcg cgggcttgac gaacaccggc agccccagtc gctcgcggtc ctcgagttcg
    10801 acggtgtcgt ggcgcggtcg gagcacgacg tggtcgccga ccggcaggcc ctcggcgacg
    10861 aggagcttct tggtgaactc cttgtccatg cccgccgcac tggccagcac acccgccccg
    10921 acgtacggca ccccagcgag ctccagcagt ccctggatgg tgccatcctc gccatatggg
    10981 ccgtgcagga cgggaaacac cacatcgacc gcggcgagca tctcgccgcc ctggccgagc
    11041 gacagcagtt gaccaccgcg aatgggatcg gcgggtaacg ccagttcggt gcccgattcg
    11101 ccgctgacct gcggcagccg gccgtcggtg atggccaggg tctccggacg accgtcggtc
    11161 agcacccagg atccgccggg tgtgatgccg accgccacga cctcgaaccg ctccgggtcg
    11221 aggttgcgca ggatgctgcc agcggaaacg cacgaaatcg cgtgctcgga gctgcgtccg
    11281 ccgtaaacaa cagcgacgcg gacgcgcccg gtggctccgg gctggtgggc agtcacaacc
    11341 tagagaggct accgctctgg ccgaatgctg catgacgcag cgctctggct tgagcagccg
    11401 aacgtgagct tgtgagccca atttcgacat tttttcgaac acaagctcac gttcgagcag
    11461 cggctttagc ccagctttaa cccagcgcct gttcgaagtc ggcgatcagg tcgtcggtgt
    11521 cctcgatgcc cagtgagata cgagcgaagc cgtcgctgac cggatcgccc cagcgggccc
    11581 ggcggtctac cgaggtatga atgccgccga agctcgtcga cgcgatcagc agcgcactgc
    11641 gctccaccag cgcgtgcacc gccgcggcgt cttccaactc caccgcgatc aggccgccga
    11701 agcgcttcat ctgcagcgtg gcgatctgat gcgacgggtc atcgggcagg ccgggatagc
    11761 gcaccgactt cacggcaggg tgtgcgcgca gcatcaccgc gagcgcctga gcgttctggc
    11821 actgtcgttc gaaccgcaga ccggcgctgc cgaggctacg cagtaccagc cacgcctcga
    11881 acgcacccag aattggcccc gcgagcagcc ggtcacgttc gagcgcttcc atcagttcgg
    11941 gctgactgcc cgcgacgtag cccgcaacga gatcgctgtg ccctgagagc gccttggtcg
    12001 cgctggccac cacgaggtcg gcgccaagcg acagcggctg ttgcccaagc ggcgttgcgg
    12061 tcgtgttgtc gacgatcagg atggcgccgc gaccgcgaca ggtcatcgcc aaatggtgca
    12121 agtcaacaac gtccaggccg ggattgacgg gggtttccgc cagcaccaca ttggcatccg
    12181 ccgacgcctc acacatctcg gcgcttcgcg cctcgacaac cgtaacaccc tgcggcgcaa
    12241 ggtattcagc tgcatagcgg cgaacctgat aatagccatc tgcgggcacc acgagcttgc
    12301 cgtcgggttt ggccatcacc cgcagcgccg acgtgatcgc cgccatgccc gaaccaaacg
    12361 tcaacgccgt tgcggcgcct tccagttcgg ccagcgccga ttccagttgg cgccacgttg
    12421 gattggagct gcggccgtag ctgtcgagcg gctccgtttc gtcaggtgac aggtaatacg
    12481 tcgacgccgg aacaggcggt tgcgataccg ggctacccgg aatcgcttcc gagccaacgg
    12541 ctttgacgct gcgggtggaa tcgccgtacg tgccgtccat cttcctactc cggtttggtg
    12601 cttcgtccca gcagtagcgc gaccgcctgg tcgaccgata atcccttgtg gcagacgcgt
    12661 tgcaccgcat cggtcagcgg catctcgacg tcgtaactcg ccgcgagcgc gagcaccgac
    12721 tcacacgagg tgacaccctc tgcgatatga ccgcccgcgg cgagcagcgc cgcctccatg
    12781 gtgtcgccct tgccgagccg ctcgccgaac gagcggttgc gcgagtgcgg ggaggtacac
    12841 gtggcgacca gatcgccgac gccggcaagc cccgccatgg ttgcgggttt ggcacccaac
    12901 gcgattccca gccgcatgat ctcggcgagg ccgcgcgtga tgatcgccgc cgcggtgttt
    12961 tcccccagcc cgacgccggc ggccatcccg caggccagtg caatcacgtt cttgcacgca
    13021 ccgccgacct cggcgccgat gacgtcggca ttggtgtacg gacggaagta cccggtcgac
    13081 agcgcacgct gcaaggcgac ggcccggccg gagtcactgc acgccaccac ggtcgctgcc
    13141 ggctgctcgt ccgcgatctc gctggccagg tttgggccgg tgacgaccgc cacccgcgcc
    13201 gggtctgccc ccgtgacctg cacgatcacc tggctcatcc gcatcagcgt gtccagctcg
    13261 atgcccttgg ccaggctcac caacgtgacg tcgtcaccga tcaggtgcgt ccagtgctcg
    13321 aggttggccc gcatggtctg cgacggcaca gcgagcagca ccgtgcacgc accggagagc
    13381 gcctcggccg ggtcgctggt cgcgcggatt gacttcggaa gctcggtgtc gtcgagatag
    13441 tgagtgttgc gccgggtgtc gttgatttcg tcggcaagtt cgggacgacg ggcccacagc
    13501 gtgacgttgt tacccgcatc agccaatacc ttggccaatg cggtccccca cgcaccggcc
    13561 cccatcaccg ccgcctcgac cacggcttca cattagcgtg gtcgcaggtg ctggcaggat
    13621 ggcgctcatg agcggcacac ggacgggcga ggccgcggat atcggactgg tgatcgccgt
    13681 aaagcggctc accgccgcca agaccaggct ggcgccggtt ttttcggccc ccacccggga
    13741 aagcgtggtg ctggcgatgc tggtcgacac gatcaccgct gcgtcggccg tcgccgccct
    13801 gcagtccgtc acggtcgtca cacccgatga ggtggccgcg gacgccaccc ggcggctggg
    13861 cgcgcgggtg ctgatcgacc cgacgcccga agggcatcgc aatccactga acaacgcgct
    13921 cgccgcggcc gaaacggcaa tacttgaaga aacgcccaat atcgttgcgc tgcaaggtga
    13981 tttgcccgcg cttcagccac aggagctggc cgaggcaatc accgctgccc ggggctattc
    14041 gcgcagcttt gtcggcgaca ggcacggcac ggggacctcg gctctgtttg cgtttggcgt
    14101 gggacttgat cctcatttcg ggcctgattc ggcccagcgc catcggcagt cgggggccat
    14161 cgaactgacc ggcgcatggc ccggcctgcg gtgcgacatc gacacacccg acgacctgct
    14221 ggtcgcccgg cgcctcggtg tcggtgctgc taccgcgcag gcgatcggtc accacaggtg
    14281 aacagcaggc gacgggatgg cccatcgcgg tagccgggtg gctggttaat aaggaatcat
    14341 cggaaacatg acggaagccg aagcccagat ccgacccgac ggcgcggagt tggtcgattc
    14401 ggcgccggag gccccacccg cggcgacgtc cccggctgtc gacaacgcgc tgcccgagga
    14461 ccggtacctc aaccgcgagc tgagctggct ggatttcaac gcccgcgtgc tcgcgctcgc
    14521 cgccgacaca tcgttgccct tgctcgaacg agcaaagttc ctggcgatct tcgcgtcgaa
    14581 cctcgacgag ttctatatgg tgcgcgtcgc gggcctcaaa cgtcgcgacg agatgggtct
    14641 gtcggtgcgc tccgccgatg gattgtcgcc gcgcgaacag ctgcgacgga tcaatgaact
    14701 cacccagaag atcgccgaca ggcatgccca cgtgttcctc gactcggtgc gaccggcgct
    14761 ggccgacgaa ggaattgtca tcgtcacgtg ggccgaactc gacgacgacg agcgctcgcg
    14821 gctctcgacc tacttccatg agcaggtgtt cccggtgttg acgccgttgg ccgtcgaccc
    14881 cgcgcacccg ttcccgttcg tgagcggcct gtccctgaac ctggcgatca ccgtcaagca
    14941 ccccgacgac ggcggtcagc atttcgcgcg ggtcaaggtc ccggacaacg tcgaccggtt
    15001 cgtcgaactc gcgcgccgcg aagatagcga ggatgtcgtg cgattccttc cgatggagga
    15061 gctgattgcc gcctttctgt cggcgctctt cccgggcctg gaagtcgttg agcatcacgc
    15121 gttccgcatc acccgcaacg cggacttcga ggtggaagag gaccgcgacg aggacctgct
    15181 tcaggcgctg gaacgggagt tggcacggcg ccgcttcggt tcgccggtgc gtctcgaagt
    15241 gtccgacgac atgaccgaga gcatgctcga gttgctgttg cgcgaactcg acgtctaccc
    15301 cggcgatgtc atagaagtgc cagggctgct cgatctgtcg tcgctgtggc agatatacgg
    15361 agtcgaccgc ccggcgctga aggatccgcc gttcgtgccc gcgaccccgc ctgcgttcgg
    15421 tgagcgcgaa acacccaaga gcatcttctc cacgttgcgt gacggcgacg tgctcgtcca
    15481 tcacccgtac gattcgttct ccacgacggt gcaacgattc atcgagcagg cggcagccga
    15541 tcccaacgtg ctagcgatca agcagacgct ctaccgcaca tccggtgact cgccgatcgc
    15601 caacgcgctc atcgacgcag ctgcggccgg taagcaggtc gtcgcgctcg tcgagatcaa
    15661 ggcgcgcttc gacgaacagg ccaacatcaa gtgggcccgt gcgctggaac gcgccggcgt
    15721 gcacgtcgtc tatgggttga tcggtttgaa gacgcactgc aagacctgtt tggtggtgcg
    15781 ccgcgagggc tccacaatcc ggcgttactg ccacatcggc acgggcaact acaacccgaa
    15841 aaccgcgcgc ctatatgaag atgtcggctt gttgaccgcg gcgcctgata tcggcgcgga
    15901 cctcaccgat ctgttcaact cgctcacggg ctactcacgc aaggactcct accgcaatct
    15961 gctggtcgca ccatacggcg ttcgcagggg catcatcgaa cggatcgagc gtgaaatcgc
    16021 ggccacccgc gacggcgccg agggccagat tcggctgaag gccaacgcgt tggtcgacga
    16081 gcaggtcatc gacgctctat accgcgcttc acaggccggg gtccgcgtcg aggtcgtggt
    16141 gcgcggcatc tgtgcgctga agccaggcgc ggccggatac tccgagaaca tcgtcgtgcg
    16201 ctcgatcctc ggccggttcc tcgaacactc gcgaattatt cacttccgcg ccatcgacga
    16261 gttctggatc ggcagcgccg acatgatgca tcgtaatctc gaccgacggg tcgaggtgat
    16321 ggcgcaagtc aaagatccac ggctgacggc acaactgaac gacgtgttcg agtccgcgat
    16381 ggaccccgcg acccgctgct gggagctggg cgtcgacggg cactggatag catcgccgca
    16441 ggagggacag acggtacgcg accatcaagt gtccctgatg gaacgtcatc ggcatccgtg
    16501 acgaccggcg tggcgcccac ccacgggctc gactgagctg caggagttga agtgccgaag
    16561 gagcttccgc tagatgcggt cccggcgagc aaggccgtac ttgctgccgg tgcagtgctg
    16621 tggcggtcca acggtgatcc gtcgtcgccg gagatcgcca tcatccaccg gccccgctac
    16681 gacgactggt cactacccaa aggcaaggtc gaccccggcg agacggaacc cgtcaccgcc
    16741 gtgcgcgagg tacacgagga gaccggctac tcgtcgcacc tcggcaggcg actggccgcc
    16801 gtcagttatc cagtcgagca gggcatcaag aaggtgcggt actgggcggc gcgcagagtc
    16861 gacggcgaat tcaatccgaa cgccgaagtc gatgaactca agtggctgcc agtggccgag
    16921 gcgctgaagc agctggagta tccgcacgat cgaaaagtgt tgcgccgctt cgccaaacaa
    16981 ccagtggaca cccaaacggt gttgatcgtg cggcacggca cggcgggcag caagtcacgt
    17041 tacaagggcg atgaccgaaa gcggccgctg gacaagcacg gccgcgcaca ggccgagtcg
    17101 ctcgtcggtg tgctgctcgc cttcggcgcc gatgtgctct acgcggccga ccgagtgcgc
    17161 tgccatcaga cgctcgagcc gctggccgaa gaacttggcg ccaccatcca gggcgagcca
    17221 ctgctcaccg aggaggcgta cgccgataac cgcaaggccg cccggcatcg cttcctcgaa
    17281 atcgcggccg cgggaggcac gcccgtgatc tgcacacagg gcaaggtgat tcccgatctg
    17341 atcgcgtggt ggagcgagcg cgacggggtg cgccccgaca agtcgcgtaa tcgcaagggc
    17401 agcgcgtggg tgatgtcgtt ggccaacgag cgcttgatcg ccgccgacca catcggcagc
    17461 ccgctagccg ccaagaagta acctccggaa acggcagtcg gcaacgcacc acggtgatcg
    17521 gcggttcctt cttcgaattg gtgccgtcgg gtggcgatgc ctatgtgctc aaacaaatcg
    17581 tgcgcgactg ggatgaatcc aaagggctcg agattttgcg tagtgtcccg atgatgatcg
    17641 cgagcatctg tccaaactgc tcgatgacta tgccgagctg ttaggacgag ccggtttccg
    17701 caattcgcgg gtgatccccc acggtaggtc cggcgtcaat cgtggagtcc gaagcggcct
    17761 agccgaactc acgacataca gacacgccgc gggtcgcggg gacccacggc gtgcccgttg
    17821 ccgaacttag cgacggccgc ccttcttggc cggcgccctc ttggcggcgg ccttcttgac
    17881 tccagcccgc ttcacggcct tcttggcggc agtccgcttg acggccttct tggcggcagt
    17941 ccgcttgacg gccttcttgg cggcagtccg cttgacggcc ttcttgactg cagccctctt
    18001 cacggccttc ttgacggcgg ccctcttgac cgcctttttg acggctgccc tcttgacggc
    18061 cttcttcgct gcggctttcc taaccgcctt gcgggcgccg cccgcggtca cgccacgttt
    18121 cactgctggt ccttccgccg ggaggcgctg tgcgccagag acaaccgctt tgaattgagc
    18181 gccgggccta aacgccggca cggaggtggg cttaactctc accgtctcgc cagtgcgcgg
    18241 gttgcgcgcg acacgggctg cacgacggcg ctgctcgaaa acgccgaatc ctgtaatggt
    18301 gacgctgtca cccttatgca ccgcacgcac gatggtgtcg acgacgtttt ccaccgccgc
    18361 ggttgcttgc cgacgatccg agcccaattt ctccgtgagt acgtcgatga gctccgcttt
    18421 gttcatgcaa aacctccgaa accagtggcc ctctttggac cgactagagg acacggtaaa
    18481 cccattgacc actgatttcc aagagccacg cgcaatttca ggtgccactg gcgaactttt
    18541 cggcaatccg cttgcccccc ttggcctcct gccgagggcc cggagaaccg ggttgagagg
    18601 tggcgatttc ggctcgcccg gcccaattcg tcacaccggc agagtgcgcg gtttccaatt
    18661 cggccgactc gactcgtacg actcgattgc atcgagtttt cgcagcgtaa ggcctatatc
    18721 gtcgagtcct tcgagcagcc gccaggcggt gtaatcgtca atgttgaacg gcaccatgac
    18781 cgttcccgcg acgatattcc gatcttgaag attcacagtg atttccatac ccggatgctc
    18841 ctcgatgagc ttccaaagca gttccacatc atcttgagaa atttctgccg ccaagagccc
    18901 cgccttgccc gcattgccgc gaaaaatgtc ggcgaatcga gacgagataa cgaccctgaa
    18961 gccgaagtcc atcagcgccc agacggcatg ctcgcgcgac gagccggtgc cgaaatcggg
    19021 tccggagacc aacaccgagc ccttgtcgaa cggcgacaga ttgagaatga acgacggatc
    19081 ggtgcgccag gccgcgaaca gtccatcctc gaaacctgtt cgggttaccc gcttcaaata
    19141 gacggcgggg atgatttggt cggtgtcgac attcgaccga cgcaacggaa caccgatgcc
    19201 tgtgtgagtg cggaacgctt gcatgacagt ctcctctcaa ttagtcaggt cggcaggaga
    19261 tgccaacgtg ccccgcacag cggtggccgc ggcgaccgca ggcgacacaa gatgcgtgcg
    19321 accacccttg ccttgcctgc cctcgaaatt tcggttggac gtcgacgcac accgctcgcc
    19381 cggtgacagc tggtctggat tcattccaag acacatcgag caaccggcct ggcgccactc
    19441 cgcaccggcg gcggtgaaga tctcaccgag gccttcggat tcggcctgcg cgcgcacccg
    19501 catcgaccct ggcacgatca gcatccgcac gccgttggcg accttgcgac cgcgcaacac
    19561 gtcggcgaca acgcgcagat cctcgatcct gccgttggtg cacgagccga cgaacacagc
    19621 gtccaccgcg atgtcacgca tcgccgttcc gggtcgaaga tccatgtacg ccaacgcttt
    19681 ttcggccgtc tgccgctcgg cgtcgtcgaa catcagctcg ggatcgggga ccgaatccga
    19741 caacgccacc ccctgcccgg ggttggtacc ccacgtgacg aatgggctca gcgtcgaggc
    19801 gtcgagatac acctcggtgt cgaattcagc gccttcgtca gtacgcaact gagtccacgc
    19861 ggcgaccgcc gcatcccagt ccgcgcccgt cggcgcgtgc ggacgtccac gcagaaattc
    19921 gaacgtggtt tcgtcaggcg ccaccatgcc ggcgcgggcg cccgcctcga tgctcatatt
    19981 gcagatcgtc atccggccct ccatcgacag cgattcgatg gcgctgccgc ggtattcgat
    20041 gacatgaccc tgtcccccac cggtaccgat tttcgcgatc accgcgagga tgatgtcctt
    20101 cgcgctgaca ccgggcggca gctggccgtc cacattgacc gccatggtct tgaacggacg
    20161 cagcggcaac gtctgcgttg cgagcacatg ttcgacctcg gatgtgccga tgcccatcgc
    20221 gagtgcgccg aatgcgccgt gagttgaggt gtggctgtct ccacacacga ccgtcatgcc
    20281 gggctgggtc agtcccaatt gcggcccgat gatgtgcacg atgccttgct cggcgtcgcc
    20341 catcgggtgc agccggatgc cgaactcttc gcaatttcgg cgcaacgtct caacctgggt
    20401 gcgtgagacc gggtcggcga tcggtttgtc gatgtcgatc gtcggcacgt tgtggtcttc
    20461 ggtggcgatc gttaggtcgg gcctgcgcac ggcgcggttg ttgagccgaa gcccatcgaa
    20521 ggcctgcggg ctcgtcacct catgtatgag gtgcaggtcg atgtagatca ggtccggttc
    20581 gcgggcggtg ccttcaccgg tgccgtgggc gacgacgtgg tcggcccaca ccttctctgc
    20641 catggtgcgc ggcgtggtca tacttgacta tctcacaatc tgggacgtta gtatctcctt
    20701 gtgagacagg atagcggcat cggcgtgctg gacaaagcgg tgggtgtgct gcacgcggtg
    20761 gcagactcgc cgtgcgggct ggccgaactc tgcgaacgca ccggcctgcc gcgggccacc
    20821 gcgcatcgac tggccgcggg cctggaagtg caccggctgc tgagccgcaa cggggacggc
    20881 cggtggcggc tgggccccgc gctgaccgaa ttggccggac aggtcaacga tccgctactg
    20941 gcagccggcg cggtggtgct gccgcgactg cgggagatca ccggggaaag cgtgcagctg
    21001 taccgtcgcg agggcacttc gcggatctgc gtggcggcgt tggaaccgcc tgcggggctt
    21061 agggatacgg tgccggtcgg aaccaggctg ccgatgaccg ctgggtcggg cgccaaggtg
    21121 ctgttggcct atgcggatgc cgcgacgcag caggcggtgc tgccggcggc gatgttcacc
    21181 gatcgcgccc tggcagaggt tcgcaaacgt ggctgggcgc agagcgccgc cgagcgtgaa
    21241 cccggcgtgg ccagcgtctc ggcgccggtg cgagatgcac gcggtgcggt gatcgccgcg
    21301 gtatcggtgt ctggaccaat cgaccggatg ggacgccgac cgggtgcccg gtgggccgca
    21361 gacctgctgg cggcggcgga tgcgctgaca cgccggctct gaaaagagaa agcgattgcg
    21421 ccggttggcg caatcgcaat tttgtagccc cgatgggatt cgaacccacg ctaccgccgt
    21481 gagagggcgg cgtcctaggc cgctagacga cggggccaga accggattgc cagcataacg
    21541 cacggggtta agcgggccta atccactgca cctgacgctc ttcgcgccaa gcgctcatcg
    21601 cacgaagctg gggtaccagg actcgaacct agaatggctg aaccagaatc agctgtgttg
    21661 ccaattacac cataccccat tggctgccta taaccgcagg tcacaggcca tttctggcct
    21721 cctcagccgg tggaaagttc ccgccgtctg ttgtatcggg ccgacgtgca gactaccaaa
    21781 gatttgaggc tgtcttttca cgcctccgct gcagcagcgg cggctgttgc gtagccccgt
    21841 cccccgcgca gccgacgcag actgcggtca cgcccgagca gttccagcga ctcgaacagc
    21901 ggtgggctga tcgatgcgcc cgtggcggcc acccggatcg ggccgaacgc ctttcgcggc
    21961 ttgagcgcta ggttctccaa caacgcgacc ttgagcgcta tttcgatctt ggccgtggtc
    22021 cactccccga cctcctcgag cgcggcgatc gcggcgtcga gcactggggc cgcatcgccg
    22081 cgcagctcct tggcggccgc cttctcgtca agcgtgaact cgctgtcgtt gaggaacttc
    22141 agcaagtccc acgcatcacc aagcacaacg atgcgggttt gcaccagccg cgccgcctcc
    22201 gcgaactgac tgccgtcgag cccagtgtcg tggccgtggg tggcgaaata cgtccgcagc
    22261 cgctcggtga agtcctcctc gctgagcagc cggatatgtt cggcgttcag cgcgtcggcc
    22321 ttcttctggt cgaagcgggc cgggttggag ttgacgtcga cgacgtcgaa cgcggcgacc
    22381 atctcgtcaa gactgaagac gtcacggtcc tctgcgatgc cccagccgag gagggccagg
    22441 tagttcagca gcccctctgg gatgaaccca cgctcgcggt gcaggaacag gttcgactgc
    22501 gggtcgcgct tcgacagctt cttgttgccg tcgccgagaa cgcttggcag atgggcgaat
    22561 tcgggcactc gctccgcgac accgatgcgg atcaacgcct gatacagggc gatctgacgt
    22621 ggggtcgacg gcaacaggtc ctcgccacgc agcacgtgag tgatcctcat gagcgcgtcg
    22681 tcgaccggat tgaccaaggt gtacaacgga tctccgctcg cgcgtgtcag cgcgaagtcg
    22741 ggcacactgc ccgcagcgaa agtcgtctcg ccgcgcacaa ggtcgtgcca cgtgatgtcc
    22801 tcgtcgggca tccgcaaccg caccacgggc gtgcgtccgt cagcgacaaa cgccgcgcgc
    22861 tgctcatcgg tgagagtgcg atcgaagttg tcgtagccca gcttgggatt tcggccggcg
    22921 gcaatgtgcc ttgcctcgac ctccccgggg gtcgagaacg cctcgtatgc ttcgcccgca
    22981 tccagcagcc gctgcacgat atcgcggtac atctcgctgc gttcggattg ccggtacggc
    23041 gcatacggac cgccgatgtc gggcccctcg tcgtagttga gcccgagcca gctcaatgcg
    23101 tcgagcagcg ccagatagct ttcctcgcta tcgcgttcgg cgtcggtgtc ctccagccgg
    23161 aacacgaagg cgccgccggt gtggcgggcg taggcccagt tgaacaaggc ggtgcggatc
    23221 aggccaacgt gtggcgtacc ggtcggcgag gggcagaacc gaactcgtac cgtcatgact
    23281 ttcctttgcg cacaacagga ttgctcagtg ttccgatgcc ctcgacggta acgctgacgg
    23341 tgtcaccgtc ctcgatgggg ccgacgccct cgggcgtgcc ggtgagaatc agatcgccgg
    23401 gcagcagcgt catcactgcc gagatccact cgacgatcgc accaatgtca tggatcatca
    23461 gcgaggttcg gctgtgctgc ttgacctcac cgttcacctc ggtgcggatg tcgaggtcgg
    23521 ccgggtcgac gtcggtgacg atccacgggc cgatcgggca gaacgtgtcg tagcccttgg
    23581 cgcgggtcca ctggccgtcc tttttctgtt gatcgcgcgc ggttacgtca ttgccgatcg
    23641 tgtagccgag gatgttctcg gctacacgcg cggcgggaac gtccttgcag ggccgtccga
    23701 tcaccgcagc gagttcgccc tcgaagtgca cagggttggc gtcggcgggc atctggatcg
    23761 gcacgcccgg cccgatgatc gcggtgttgg gcttcatgaa gatcaccgga tcgggaaacg
    23821 atccgccgcc catctcggca acgtgcgcgg cgtagttctt gccgatgcag accaccttgc
    23881 tcgccaggat gggcgctagc aggcgcacgt cggcgagcgg ccattgccgg cctgtgaagt
    23941 tgggagtgcc gaatgggtgc tccgcgattt ccttcgcgat tgcctcgttg gggtcgtcgg
    24001 tggctccttc gatgctgacg aacgcaactc catcgggact ggcgattcgg ccaagacgca
    24061 tgatgtcagc ctagtggccg aggtcggggc ggccgcgacc cgccatgtgt ctcactattt
    24121 ggaaagatgg ttcagcatcc tgagacgcct gtgcgagcat ggtcgggtgt ccatcgaatc
    24181 gatcagctcc gtgcgtcgct ggtcgatgtt ggtcatcgcg ctaacggcga ccctgtgcgc
    24241 aaacgtcttc atcaacggtg cggcattcct catcccgacg ttgcacaccg aacgcggact
    24301 cgatctggcg aaggccggcc tgctgtcgtc gatgcccagc ttcggcatgg tgatgacgct
    24361 gatcgcgtgg ggctacgtcg tcgaccgggt cggtgagcgg accgtgctgg cactgggttc
    24421 ggcgctgacg gcagccgcgg cgttcgcagc ggcgtcggtg caatcgctcg tcgccgtcgg
    24481 cgcctttctg ctgcttggcg gcatggcagc tgccagcagc aactcggcga gcggccgact
    24541 ggtggtcgga tggtttgcgc ccgagcggcg cggcctggtg atgggcattc ggcagaccgc
    24601 gcagccgctg ggagtcggat tgggtgcctt ggtgattccc cgtctcgcgg aaagccacgg
    24661 cacctcggtg gcgctcctgt tccccgctgt ggtgtgcgcg gtgtccgcag cggtgtgcgc
    24721 ggtggcggtg atcgacccgc cgcggccgcc gcggcacgag gccgacgaag ccgatctggc
    24781 caacccatat cgcgggtcgt cggtgctgtg gcgcatccac gccgtgtcgg tgctgctggt
    24841 ggttccgcaa gtggtggtgt ggacgttcac gctggtctgg ctgatgagcg accgcggctg
    24901 gtccgcagcg tcagcgggcg cgatggtgac ggcggcccaa atattgggcg ccggtggacg
    24961 aatcgctgcc ggacgttggt cggacatcgc cggttcgcga ctgcgcccga ttcgcaccat
    25021 cgcgcttgcg gcagcggcgg cgatgggtct gctggctgtg acggactggt tgaactcccc
    25081 gctcagtgtg gcggtgatgg tgatcgcctc ggtgatcacc gtgtccgaca acggcttagc
    25141 gttcacggcg atcgccgaga tcgcgggccc gttctggagc ggccgggcac tcggcacgca
    25201 gaacaccagc cagctgctga cggccggcgt cgcaccgccg ttgttcggtg gcctgatcgg
    25261 aatcgcaggc tatcccgtcg cgttcgcggt gtgcgcgctg tttccggtca tcgcgatgcc
    25321 gttggttccc gtcgattccg acccgctgcg ccaggctgat acctcgagat gaccgtcatg
    25381 ccgtctcgag gttctcggca catcgggtca acgccgtgac gagttggcgt cgcgtggtcg
    25441 gcgagatccc ctcggtcatc gctgattcca cacccatcac tgccgtgtga gctgccgaca
    25501 gccttttggt gcctgcggcc gtcaactcca gcgaccgggc ccgccctcgc gctgggcgat
    25561 cggattccac tatgagaccg gccgaccgca gcccgaacag cacgtcctgc agcgactgcc
    25621 gggtcacaaa gcacagcctc gacagttccg atgccgacgc atcgggatgg tcagcgaggg
    25681 cgcggagaac ggcgtactgc gccatcgaca cgccaaccga ccgcagcgcg gcgtcgctgc
    25741 ggcgtcgcaa cgactgctgc acacgcttaa ccagatagcc gaccgcgcgt tccgtctccg
    25801 actcagccat gacaggaacc ttacatgagt ggtatggtcg ttgtaaggaa cctgacatgg
    25861 aggtaaagat gacgaccatc gagcagaaca gcgcatacgc caccttgatc aacgtcttca
    25921 ccgtcgaacc ggaccgggcc gcggaactcg ccgcgctgtt aaacaccgcc accgaagaag
    25981 tgatgcggca ccggcccgga ttccggtcgg ccaacatcca cgtgagcacc gacggcagcc
    26041 gggtcgtgaa ctacgcgcag tgggacagcg ccgacgcata ccggtcgatg ctcaaagatc
    26101 cgatagcgca gcggcacatg tcagaagcgg ccgcgctggc gatcagtttc gacccgcacc
    26161 tctacaccgt cgaatcggtg cacgagcgct agaggttggc cgcgatgctg tcgccctcga
    26221 cggtgtgacg cgactcgata gccaacgaga cgtcctttcg gttgatgctg gcctcgtcag
    26281 tccagcgcgg cggcatcgac gaagcgcaca gcgatcttcc tgcccgccac aatcgacgca
    26341 ccgcactgat cgaggcggca ctcgctccgg cccgcagtca attcacaacc accgcactca
    26401 agtcactgac gaggtcgctt gcgctcatca tcgggaccga atccatggtc gtcctcaaag
    26461 atgttctgca actcgacgaa cccgccgctc gcaaggtgcg gcgctgggcg attcgcgcac
    26521 tcgtcgaggc cgcacaaaag ccccgctcgc gcaattgagc ggaacgtcat ggtggccagg
    26581 tgatcctcga cggccttgtc gacgcgtgcg gcgagccgat tctgtctcca ctgttgcgta
    26641 gacccggggt cgtcttatcg agcgccggaa tcgccggata cggcgcggca ttcgcggtgt
    26701 gcgcactgtt tccgctaatg gcgctgccac tggttcccgt agttgccgat ccgctgatga
    26761 aaggccgtgg ctgagcatat gctcggccca tgacgggggc cgcaatagac atcattaacg
    26821 actacttcac ctgcgagttc accaccttgt cacgcgacgg atcgccgcac gcgtggcccg
    26881 tctcaccaag gttgttgacc gacggacggc tattgctgac cacgagcatc ggactgccgc
    26941 agaaggcgtt caatgttcgc cgcaacccga aggtgagcct cttgttctcc gaaccgacgg
    27001 gcagtggaat caatcaaccg ggggcggtgc tcatccaggg cgatgccacc tcggaggatc
    27061 gaatcgtcac ggatccgttg tcagacccgg acttcgcagc actcgcggag acggtgtcca
    27121 cgcgtcaacc tgctggcaac ctctggacta cgtggctggg ccgccgattg tggtggtcgt
    27181 actacatgcg tctcttgatc tacgtgacac cgcggcgggc attcttctgg ccgacccggg
    27241 acttcactag ctcacccgag gaactggacc tgagcgaggt gcgccgtgtg ggctgaagcg
    27301 gccaaatggc tcggaaagtt tgatgaagcg gtgttgaccg ccctcgacat cgacggctat
    27361 cccttcagcg ttcgcgtcga cacccgcacg tacgacgccg acacgggtcg gctacccgcg
    27421 gcgctgccgg gtgtgctgcg tgccgccgaa agtccagcca gcttgttatg ccactaccac
    27481 gacgaaaaaa tgtggaagct caaggccatc caggtcaagg gccggcttga aaagcgcaat
    27541 gacgcgtggg acttcgtcac gacgtcgttc acaccgccgt cgaaattggc gctgttcgct
    27601 tttctcaaag gcacagggac cgcagcccag aaatatctgg atcggcgtgg tctcgagcgc
    27661 cccaaagtga actgggttgc cgtaaacgaa attaagcgcc gcgtgtccga ggcgaagcgc
    27721 tagaggttgg ccgcgatcct gtcgccaacc tgtgcggtgg acagcttttc gtcgccacgc
    27781 gtggccaggt gatgctcgac agccttgtcg acgcgtgcgg cggcgtcgtc ctcaccgacg
    27841 tgggccagca gcagcgccac cgacatgatc gccgcggtcg ggtcggcgat gccctggcac
    27901 gcgatgtccg gtgcgctgcc gtgcaccggc tcgaacatcg acggattcgt ccgcgtcgca
    27961 tcgatgttgc cgctggcggc taggccgatg ccaccgcaca cggccgcagc gagatcggtg
    28021 atgatgtcgc cgaacaggtt gtcggtgaca atcacgtcga accgtcctgg atccgtgacg
    28081 aggtgaatcg tcgccgcgtc cacatgctga tacgcgattt cgacatcggg gtattcggtg
    28141 gcgatctcct gcacggtgcg ccaccacagc gcgccggcga aggtcagcac gttattcttg
    28201 tgcaccagcg tcagatgctt gcggcgccgc tgcgctcggc ggaacgcgtc gtgcaccacg
    28261 cgctgtacac cgaacgcggt gttgacgctg acctccgtag cgatctcgtg cggcgtgccc
    28321 acgcggatcg ctcccccagt gccggtgtat gggccctcgg ttccctcacg gacaacgacg
    28381 aagtcgatat cgggattgcc cgccagcgga ctgttgacgc cgggatacag ccgcgagggg
    28441 cgcaaattga catggtggtc gagcgcgaac cggatgcgca gcagcagacc gcgttccagc
    28501 acaccgctcg gcaccgacgg gtcgccgatc gcgccgagca ggatcgcgtc gtgcgctttc
    28561 aactcatcga tcaccgagtc gggcagcacc tcgcccgtcg cgtggtagcg ccgcgcaccc
    28621 aggtcatagc cggtcttttc gatgccgggc acgaccgcgt cgagcacctt gagggcctca
    28681 ccgaccacct cggggccgat gccgtcgccg gcaatgatcg ccaatttcac aagagatcca
    28741 ccacttccag aagtttggcg ccgacggcct cggtgatcgt ggcgcgaaca tcggcgggaa
    28801 cgtcacggtc gatccgcagc agaatcgtcg cgcccggccc ttcggcgtcc tcactgagct
    28861 gtgccgcgtg gatgttgatg tcagcggcgc cgagcagcgt accgatcttg cccagcgtcc
    28921 cgggcttgtc gagatagtga atgatcaggt tgatgccctc ggcgcgcaga tcgaagttgc
    28981 ggccgttgat ctgcacgatc ttttccacca gttgcggacc cgacagcgtg ccggcgacgt
    29041 tggtgaccga tccgtcggag aacaccgcgc ggacatcgac cacgctgcgg tggttcgggc
    29101 tctcgctggc ggtgctgatg tcggcctgca cgccgcgctc ggcggccagc gccggcgcgt
    29161 tgacgaacgt caccggatcc tcgatgaccg cggagaacaa cccgcgcagc gccgaaagtc
    29221 gcaacacctc aacgtcttcc gaggcaagct caccgcgaac ctggacggac aacgacagcg
    29281 gcacctcggt ggacagcacg ccgacgagca gacccagctt gcggaccaga tcaagccatg
    29341 gcgccacctc ttcgccgacg accccaccgc ccacgttgac agcgtcgggt acgaattcac
    29401 cggccagcgc cagcttcacg ctttcggcga cgtcggtgcc cgcccggtcc tgtgcctcgc
    29461 cggtggaggc gccgaggtgt ggcgtgacga cgacctgagg cagatcaaac agcggactgt
    29521 cggtagtcgg ctccttggcg aacacgtcga ggcccgcagc ccggacatga cccgaccgaa
    29581 cggcctctgc caacgcttcc tcgtcgacca ggcccccgcg cgcggcgttg acgatgatca
    29641 cgccgggctt ggtcttggcc agagcttcct taccgatcag cccggcggtc tcttgtgtct
    29701 tgggcaggtg caccgagatg aagtcggcgc gcccgagcag ttcgtccagg gtcagcagct
    29761 cgatgccgag ttgcgcggcg cgcgccgccg acacatacgg gtcatacgcg acgacgtgcg
    29821 tgccgaacgc ggccagccgc tgtgcgacca gctgcccgat gcggccgagg ccgacgacgc
    29881 cgacggtctt gccgtagatc tcggtgccgg agaacttcga ccgcttccac gtgcgttcgc
    29941 gcagcgacgc gtctgccgca gggatctcgc gcgcggcgga gagcagcagc gccagcgcat
    30001 gctcggcggc gctgtggatg ttcgatgtcg gcgcgttgac gaccagcaca ccgcgggcgg
    30061 tggcggcgtc cacgtcgacg ttgtccaggc caacgcctgc acgggcgacg atcttgagct
    30121 tcggcgcggc ggcgagcacc tcggcgtcga ccgtagtcgc cgagcgcacc aacagcgcgt
    30181 ctgcctccgg cacggcggcc agcagcttct ctcggtccgg gccgtcgacc cagcggactt
    30241 ctacctggtc accgagggcg gcgacggtcg actcagccag cttgtcggcg atcagtacaa
    30301 cgggaagact cacgtccaca cagcgtagtg ggcttcaatt agcgggtgga cgtcaccgtc
    30361 gtcggcagtg gacccaacgg cctggcggcc gccgtcatct gcgcccgggc cggtctatcg
    30421 gtgcaggtgt tcgaggcgca gccgactctg ggcggcgggg cgcgaacgtt agcggacccg
    30481 gagttctcgg gggtagcgca cgacatctgt tcggctgtgc atccgctggc gctggcgtca
    30541 ccgtttctcg cggagttcga tctgccgtcc cgcggcgtca cgctgaaagt ccccgaggcg
    30601 tcctacgcca acccgctgcc gggccggccc gccgcggtcg gttaccacga cttggaccgc
    30661 acctgcacag agttgacgaa cggcgagtcg tggcgccgtc tgctgggccc gcttgttgaa
    30721 cgcgacgacg ccgtgctcaa actgctgctc ggcgacaagc gttcgattcc gaccgatccc
    30781 atcgccgcca tccgagtcgc acggcggctg ctcgaacagg gcacgccggc atggcgggca
    30841 ctctccggcg tcgacgcccg cgcattgttc accggtgttg ccgcacatac gatttcgcag
    30901 atgccgtcat tggtgtcgtc tggtgctggg ctgatgctgg ccacgctggc gcacacggtc
    30961 ggctggccga tacccgtcgg cggcagtcaa gccatccccg acgcgttgat cgccgacctg
    31021 caagcgcacg gcggcaccct gacggccgac gaggagatca ccgaacctcc ttcgggcgtc
    31081 gtgcttttcg acactgctcc cacggcactg ctctcgatct accgggaccg catgccgata
    31141 cgatacgcaa aggcgttgca gcgctatcgc ttcggacccg gcgtcgcgaa agtcgacttc
    31201 gtgctgtcgg atgagattcc atggaaagat tcccggctcg ccggtgcccc gacgctgcac
    31261 atgggcggca cccgcgagca gatggcgcac gccgagcgcg aggccgccgc gggcaggcat
    31321 gcggaatggc cgatggtgct tgcggcgctg ccgcatctgg ccgatccgaa acgaatcgac
    31381 gcacagggac ggcgcccgct ttggacgtac gcgcatgtgc cgtcaggctc acccgtcgac
    31441 cagagcgaaa ccatcacaaa gattttcgag cgcttcgcgc ctggttttcg cgatgtcgtc
    31501 gtggcggccc ggtcggtgcc tgcctcgcgg ttggccgagc acaacgcaaa ccttgtcggc
    31561 ggcgacatcg gcgtcggtgg caataccttg ttccacgcgc tggccgg
//
LOCUS       DASRJA010000129         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3648510,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000129 DASRJA010000000
VERSION     DASRJA010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3648510"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..84
                     /locus_tag="VFC01_05905"
     CDS             <1..84
                     /locus_tag="VFC01_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZN79211.1"
                     /translation="DAVAAADEVTASNIEDGVARVLERWWL"
     gene            complement(87..1703)
                     /locus_tag="VFC01_05910"
     CDS             complement(87..1703)
                     /locus_tag="VFC01_05910"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZN79212.1"
                     /translation="MPCRRPAPSLLFTAIAGTIVLLPWAINGLPGDGDNRPSLPAPQL
                     AQQPLTGLGGGETIREVHQATPFSIVALTGEDLTGTSAKVRAKKADGSWGPWYEAETL
                     DGVGPDVAAPRGTEPIFVGRTTTVQISVTRPVGAAPTGASPKAPAKPGLGYVPANVEQ
                     PFAQNINAVLISPPQAPVDIVPLPSAVTVPGQPPNIIGRAQWGADEAMRCGNTVYDNG
                     IRAGIVHHTAGSNDYAPQDSAGIVRSIYEYHTRTLGWCDIAYNALVDKYGQVFEGRAG
                     GMDRPVEGSHTGGFNRNTWGVAMMGDFDVVPPTPIQLRTTGRLLGWRLGIDHIDPRGT
                     VVLPSAGGSFTKFAVGATPTLPTIFTHRDVGNTDCPGNAAYALMDRIRDIAARFNDPP
                     GPADLAESLRGGAIFARWDSMGAMNSLLAAPTSPEASGEGPTRYVTFDNGAVYWSPQT
                     GAEPVTGAIYKAWGSLGFERGALGLPTSGEIQEPQWIVQNFQHGTLNFDREKGTVTRV
                     IDGVPLELPPPSSEAAPIQLERFTPPINPT"
     gene            complement(1787..>2302)
                     /locus_tag="VFC01_05915"
     CDS             complement(1787..>2302)
                     /locus_tag="VFC01_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79213.1"
                     /translation="PALGDGALTNPALTNPVPAANAALPNPALTSPTGLAPGLTTPPA
                     TGTDPALASPISNAGALPAPGEVPISAPIGLDPAAGTYPLLGDPSLAAAPATGGSSGL
                     LGDLSSAASQLGAGQAIDLLKGVLMPAIMSAVKPAGAPAADAAAAATSAVPAAAAALP
                     AAAPALPVPGI"
BASE COUNT          349 a          765 c          822 g          366 t
ORIGIN      
        1 gacgcggtgg cagcggccga cgaagtcacc gcgtccaaca tcgaggacgg cgtcgcccgc
       61 gtactcgaac gctggtggct ctgacgtcag gtcgggttga tcggcggcgt gaaccgctcg
      121 agctggatcg gtgcggcctc actggacggc ggcggcagct ccagcgggac gccgtcgatg
      181 acgcgggtca ccgtgccctt ctcacgatcg aaattcagtg tgccgtgctg gaagttctgc
      241 acgatccatt gcggctcctg aatctcgccg ctcgtcggca ggccgagcgc gcctcgctcg
      301 aagcccagcg acccccaggc cttgtagatc gcaccggtga cgggctcggc acccgtctgc
      361 ggcgaccagt acaccgcgcc gttgtcgaac gtgacgtatc gcgtcgggcc ctcgcctgac
      421 gcctccggcg aggtaggcgc agcgagcaga ctgttcatcg cccccatcga atcccagcgc
      481 gcgaaaatcg caccgccacg cagcgattcg gccagatccg cggggcccgg cgggtcgttg
      541 aacctggcgg cgatgtctcg gatccggtcc atcagcgcat acgccgcgtt gccggggcag
      601 tccgtgttgc cgacgtcgcg gtgagtgaag atggtcggca gcgtcggcgt cgcaccgacg
      661 gcgaacttgg tgaacgaccc gcccgcagaa ggcaatacga ccgtgccccg cgggtcgatg
      721 tggtcgatcc cgagccgcca gcccagcagg cggccggtgg tccgtagttg gatgggtgtc
      781 ggcgggacca cgtcgaaatc gcccatcatc gcaacacccc aggtgttgcg gttgaagccg
      841 ccggtgtgcg aaccctcgac aggtctgtcc atgccgcccg cgcgaccctc gaagacttgc
      901 ccgtacttgt cgaccagcgc gttgtaggcg atgtcgcacc agcccagcgt gcgggtgtgg
      961 tactcgtaga tcgaccggac gatgcccgcc gaatcctgcg gtgcgtagtc attgctgccc
     1021 gctgtgtgat ggacgattcc agcgcgaatt ccattgtcgt acaccgtgtt tccgcaccgc
     1081 atcgcctcgt cggcgcccca ctgtgcgcgg ccgatgatgt tgggtggctg accgggaacc
     1141 gtgaccgctg acggcagcgg cacgatgtcc accggcgcct gcggtgggct gatcagcacc
     1201 gcgttgatgt tctgcgcgaa cggctgctcc acgttggcgg gcacgtagcc caggcccggc
     1261 ttggcgggtg ccttcggcga tgctccggtg ggtgccgcac cgaccggtcg ggtgacagag
     1321 atctgcaccg tcgtggtgcg gccgacgaaa atgggttcgg tgccccgggg ggccgccacg
     1381 tcgggaccca ccccatccag cgtctcggcc tcataccagg gcccccatga accgtcggcc
     1441 ttcttcgccc tcaccttggc cgaggtgccg gtgaggtcct cgccggtcag cgcaacgatc
     1501 gagaacggcg tggcctggtg gacctcgcgg atggtttcgc cgccaccaag gccggtcagc
     1561 ggctgctggg cgagctgagg tgccggtagt gacggcctgt tatcgccgtc gccgggcagg
     1621 ccgttgatcg cccacggcag cagcacgatt gtccccgcaa tggcggtgaa caacagcgac
     1681 ggcgcgggtc gacggcacgg cacggcccga tgttacgtat gtgtcagttg ttaccactgt
     1741 tccgacacgg ttgttggacc gactaaatgc cggctgctta ccggatctag atgcccggaa
     1801 ccgggagtgc cggcgcggct gccggaaggg cagcggccgc tgcagggacg gccgaggtgg
     1861 ccgccgcagc cgcatccgcc gccggggcgc cagccggctt gacggccgac atgatcgccg
     1921 gcatcaacac gcccttgagc aagtcgatgg cctggccggc accgagctga ctggcggcgc
     1981 tggacaagtc gcccagcaga ccgctgctgc cgccggtggc aggggccgcc gcaagtgacg
     2041 gatcgccgag gagcggatac gtgcccgcgg caggatccaa cccgatgggt gccgagatcg
     2101 gcacctcgcc cggggcaggc agggcacccg cgttggagat cggtgacgca agggccggat
     2161 cggtgccggt agcaggcggc gtggtcaggc cgggggccag gcccgtcgga ctggtcagcg
     2221 ccgggttggg cagtgccgcg ttggcagcag gaactgggtt ggtcagcgcg ggattggtca
     2281 gcgccccatc gccgagcgcg gg
//
LOCUS       DASRJA010000130         7855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1028756,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000130 DASRJA010000000
VERSION     DASRJA010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7855
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028756"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VFC01_05920"
     CDS             <1..488
                     /locus_tag="VFC01_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN79214.1"
                     /translation="AKVAGMVERAPGEGGRIVAGGSAPDRPGSFYLPTLIADVDEQSE
                     VYRDEVFGPVLTVRSFVDDDDALRQANDTEYGLAASAWTRDVYRAQRASREIHSGCVW
                     INDHIPIISEMPHGGVGASGFGKDMSDYSLEEYLTIKHVMSDITGVADKGWHRTIFTK
                     R"
     gene            589..1266
                     /locus_tag="VFC01_05925"
     CDS             589..1266
                     /locus_tag="VFC01_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN79215.1"
                     /translation="MKYGQGWRPPEVDWSSTRGRILLAASALFAQRGYFGTSTRDIAD
                     EVQIRQPSLFHHFEAKHEIFRTLVELDLGPSIDRLRSRLAEDSTWAEKLHLSIACDVR
                     EALIQPFDARGLYQDAVLGLDEFADEREGIALFHELVEHVISSGREAGEFVVFEPSFV
                     QRAVNGVLFETLREQGGPGGRIRERRPLQAADFVLRALLVDQDTLDKVRVVTQQRLGE
                     IEAAAVP"
     gene            1435..2286
                     /locus_tag="VFC01_05930"
     CDS             1435..2286
                     /locus_tag="VFC01_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN79216.1"
                     /translation="MKKSAIAGAIVGAIALAVSGCSPSSDESTPTSTGPAKVAPPAIL
                     QAGTLKICAPNDGTPPSVYHDGTGALVGSEVDLGKSIAAKLGLKPDFVESAFAAVIPT
                     LQAKQCDVIMAQLYIKPEREKVVDFVPYVYSGTGIAVSKESPADISGMDDSLCGKKVM
                     VAVGTTAESLSQEQSDKCTAAGKPAIDINRNNHADVSLQQLQNGQVDAFLDTAETLGY
                     YATKTGAQIQLAGQPFGTIKIGAATLKGNTELHNAIQQALNELESDGTYAKILDQWGQ
                     SDLSIQK"
     gene            2297..3082
                     /locus_tag="VFC01_05935"
     CDS             2297..3082
                     /locus_tag="VFC01_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZN79217.1"
                     /translation="MHFDWQFFWKALFTPSEPFLDGLILTIVISVVAMILATLAGLVI
                     ALMGRSRFTGLRWCASVYIWVIRGTPLLVQLVLIYTGLAAVGIYQFHDVSLWGLSVKA
                     AVQAAIIGLMINESAYIAEIIRAGLDSVPKGQFEAAASLGMKPGKLMRWIIVPQSLRV
                     MVPPLGNSFNGMMKTTSVLSVIGVSEMFLVAQSISSYTFHTFEIFIVAAIYYLALTTI
                     WTFIQAYIENRLARQLGIEQRVRITQRLLGARRAGTPALQAHG"
     gene            3112..3891
                     /locus_tag="VFC01_05940"
     CDS             3112..3891
                     /locus_tag="VFC01_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZN79218.1"
                     /translation="MSVTAESTAPVVSVQRASCTLGGRKVLDDVSLDVMRGQVVVLIG
                     PSGAGKTTLLRSLNHLEPLDSGEVLIGGVPIAHRDTTSKKKVSTSELARRRREIGFVF
                     QHFNLFPHMTAAENVWNGPVRVLGTPKDKARQDALALLERVGLSDKAESRPSQLSGGQ
                     QQRVAIARALAMRPRVMLFDEPTSALDVEMVGEVLEVMRELARDHMTMIVVTHEMRFA
                     REVADRIVVMDAGRIIEDAAPDQLFGHPQHERTKAFLSTIH"
     gene            3954..5546
                     /locus_tag="VFC01_05945"
     CDS             3954..5546
                     /locus_tag="VFC01_05945"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZN79219.1"
                     /translation="MSTQSAPTSFTGALATPHALATEAGMNAYRDGGNAIDSAIAAAA
                     VLTVVYPHNVALGGDLIALVRTPNGETHCVNASGWAGSAVDVASLRARHGRWLPARGA
                     DSVTVPGGIRGWETLRRFGARLTWDQTLRAAESTARDGAPVAPSLASHIADPENADLF
                     GVEDFDRVFRPAGRNLQKGDEFAQPALADTFSVLREQGPDAFYEGQLAERSIAYLRSC
                     GSSLTVEDFAAFHPDTVEPISVPFRGLTVLTSPPNTHGFLLLRALRAVDELGITDPLD
                     DGLGTLLRVFHRGNALRSSYLADPRHASVDVDALVNDGLDELAELGEMPSGPALVPHG
                     DTVGVAAADGDGYAVSLIQSVYHAFGSGLIDPVTGVLFHNRGTGFSLDEASLNVIAPR
                     KRPAHTLMPVMTTHHRSPSHVLATMGGQGQPQILAQVLLRAVGGAGAESAVAAPRAIV
                     GLQVDGATTDSVTAESDIPAAARASIGRAGLIATDVAAHTESLGQTNVVFADAKGSMM
                     AASDPRSDGAAVVAHYPRFVQL"
     gene            complement(5543..7049)
                     /locus_tag="VFC01_05950"
                     /pseudo
     CDS             complement(5543..7049)
                     /locus_tag="VFC01_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510112.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            7103..>7855
                     /locus_tag="VFC01_05955"
     CDS             7103..>7855
                     /locus_tag="VFC01_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN79220.1"
                     /translation="MTASTRVDEFEELRPHLLAVAYRLTGTVADSEDIVQDAWLRWDG
                     TSESIVDLRAWLTTVVSRLSLDRLRSAAKRRETYVGEWLPEPVVTGFDSNDPLAAVVA
                     ADDARFAAMVVLERLTPDQRVAFVLHDGFAVPFGEIASVLGVSEASARQLASRARRAV
                     ASAPPAVADDTHNEVAGSLMAALAEGDMKAVVRLLHPDVTFTGDANRRAPTAARVIRG
                     PDKVARFLFGLARRYGPGWLAANQLALVNGQLG"
BASE COUNT         1414 a         2484 c         2601 g         1356 t
ORIGIN      
        1 gcgcgaaagt ggccggcatg gtggagcggg cgcccggcga gggcgggcgg atcgtcgcgg
       61 gcggcagcgc accggatcgg ccggggtcgt tctacctgcc gacgctgatc gccgacgtcg
      121 acgagcagtc ggaggtgtac cgcgacgagg tcttcggccc ggtgctgacg gtgcgctcgt
      181 tcgttgacga cgacgatgcg ctgcggcagg cgaacgacac cgagtacggt ttggccgcgt
      241 cggcgtggac gcgcgacgtg taccgggcgc agcgcgcctc gcgcgaaatc cactccggtt
      301 gtgtgtggat caacgaccac atcccgatca tcagcgagat gccgcacggc ggcgtcggcg
      361 cgtcgggctt cggcaaggac atgtcggact actcgctcga ggaatatctc accatcaagc
      421 acgtgatgag cgatatcacc ggcgtagccg acaagggttg gcaccgaaca atcttcacga
      481 agcgctgata ccgcgaacgt tccttaccgc tcgatatttt cgcgattctt cgagcggtac
      541 ggaacgttcg tcgctggatc gcgattgatt cggtgacaca atcgagccgt gaagtatggg
      601 caaggttggc gaccgccgga ggtcgactgg tcgtcgacga ggggccgcat cctgcttgcg
      661 gcgtcggcgt tgttcgccca acggggttac ttcggcacgt cgacccgcga catcgccgac
      721 gaagtacaga tcaggcaacc gtcgctgttc catcattttg aggcgaagca cgaaatcttc
      781 cggacgctgg tcgaattgga cctcggcccg tcgatcgacc gcctccgctc tcgcctcgcc
      841 gaggactcga cgtgggccga gaagctccat ttgtccatcg cgtgcgacgt gcgcgaggcg
      901 ttgatccagc ccttcgacgc gcgtggtctc taccaggacg cggtgctcgg actggacgag
      961 ttcgccgatg agcgcgaggg cattgcgctg ttccacgagt tggtcgagca tgtcatctcc
     1021 agcggccgcg aggccggcga gttcgtggtc ttcgaaccga gtttcgtgca gcgtgccgtg
     1081 aacggcgtgc tgttcgagac ccttcgcgag cagggtggac ccggcggccg cattcgcgag
     1141 cgccgcccct tgcaggcggc cgacttcgtg ctgcgggcct tgctcgtcga ccaggacacc
     1201 ctcgataagg tccgcgttgt cacgcagcag cggcttggcg agattgaggc cgccgcagtg
     1261 ccctgacctc caggaacgcg gttagctcgt taaatctgtg caacaaagcg cgatgaattt
     1321 cgcgcaaagc tagcctatcg gtcgataggc gcctatcgtt tgataggccc gcggtgtggc
     1381 gtgcgtcacc gacgctccgc cctgatcgcc atccgcccga ctcatggagg aaccgtgaag
     1441 aagtccgcca tcgccggcgc catcgtcggc gccatcgccc tcgcagtgtc cgggtgttcg
     1501 ccctcatccg acgagtcaac gccgaccagc accggacccg cgaaggtcgc cccacccgcg
     1561 atcctgcagg caggcacgct gaagatctgt gcgccaaatg atggcacgcc gccaagcgtt
     1621 taccacgacg ggaccggcgc gctggtgggc agcgaggtcg acctcggcaa gtcgatagcg
     1681 gcgaaactcg ggctcaaacc cgacttcgtt gagtccgcct tcgcggcggt catccccacc
     1741 ttgcaggcca agcagtgcga cgtcatcatg gcccagctgt acatcaagcc ggagcgcgaa
     1801 aaggtcgtcg acttcgtgcc ttacgtgtac tcaggcaccg gtatcgcggt gtcgaaggag
     1861 agcccggccg acatctccgg aatggatgac agcctgtgcg gcaagaaggt gatggtcgcg
     1921 gttggaacga cggccgaatc gctgtcccag gagcagtccg acaagtgcac ggccgcaggt
     1981 aaaccggcga tcgacatcaa ccgcaacaac catgccgacg tgtccctaca gcagttgcag
     2041 aacgggcagg tcgacgcgtt cctcgacacc gcggagacgc tcggctacta cgccaccaag
     2101 accggtgcac agattcagct ggccggccag cccttcggca ccatcaagat tggcgccgcg
     2161 acgctgaagg gaaacaccga gctgcacaac gcgattcagc aggccctcaa cgagctcgaa
     2221 agtgacggca cctacgccaa gatcctcgac cagtgggggc aatcggacct cagcatccag
     2281 aagtaagcgc aggaccatgc acttcgattg gcagttcttc tggaaggcgt tgttcacgcc
     2341 gagcgagccc ttcttggatg gtctgatcct gaccatcgtg atctcggtgg tcgccatgat
     2401 cctggcgacg ctggccggtc tcgtcatcgc attgatggga cgctcgcgct tcaccgggct
     2461 gcgctggtgt gccagcgtgt atatctgggt gattcgcggg acaccgctac tggtgcaact
     2521 tgtgctgatc tacacggggc ttgccgcggt cggcatctac cagttccacg acgtctcgct
     2581 gtggggcctg tcggtcaagg cagcggtgca ggccgcgatc atcgggctga tgatcaacga
     2641 aagcgcttac atcgccgaga tcatccgtgc cggactggat tccgtgccga aaggtcagtt
     2701 cgaggccgcg gcatcgctcg gcatgaagcc gggcaagctg atgcggtgga tcatcgtgcc
     2761 ccagtcgctg cgcgtcatgg ttccgccact cggtaactcc ttcaacggga tgatgaagac
     2821 cacgtcggtg ctgtccgtga tcggcgtcag tgagatgttc ctggttgctc agtcgatcag
     2881 ttcctacacc ttccatacgt tcgagatctt catcgtcgct gcgatctatt accttgcgct
     2941 gacgacgatc tggacgttca tccaggccta tatcgagaat cggctggccc gccagctcgg
     3001 catcgagcag cgggtccgga tcactcagcg gctcctcggc gcacgtcgcg ccggcacccc
     3061 ggccctccag gcgcacggat gacccggatc atgaacgtta aggagtcgac gatgagtgtc
     3121 accgctgagt ccacagcgcc ggttgtgtcg gtgcagcggg catcgtgcac gctgggaggc
     3181 cgaaaggttc tcgacgacgt ctcgctcgat gtcatgcgcg gacaggtcgt cgtacttatc
     3241 ggaccctccg gggccggtaa gaccacgctg ctgcggtcgc tgaaccacct cgaaccgctc
     3301 gacagcggcg aggtcctgat cggcggagtg ccgatcgccc accgcgacac gacgagcaag
     3361 aagaaggtca gcaccagcga gctcgcccgg cgccgccgtg aaatcggatt tgtcttccag
     3421 catttcaacc tgttccccca tatgacagcg gccgagaacg tgtggaacgg cccggtgcgg
     3481 gtgttgggga caccaaagga caaggcccgt caggatgccc tggcgctgct ggagcgcgtc
     3541 ggtctatcgg acaaggcaga atcgaggccc agtcagctgt cgggtgggca acaacaacgg
     3601 gtcgcgatcg cgcgggcact ggcgatgcga cccagggtta tgctcttcga cgagccgacc
     3661 agcgcgctgg acgtcgagat ggtcggcgag gtcctcgaag tgatgaggga actcgcgagg
     3721 gaccacatga cgatgatcgt cgtcacccac gagatgcggt tcgcccgtga ggtggccgac
     3781 cgcatcgtcg tcatggatgc gggccgcatc atcgaggacg ccgctcccga ccaactcttc
     3841 ggccacccgc aacatgagcg gaccaaggcg ttcctgtcca cgatccactg accgggctcg
     3901 cgttcacccg aaacccgttg tcccgctgcg gttacagcaa ggagtttctc accgtgtcca
     3961 cccaatcagc gccaacgtcg ttcacaggtg cgctggccac gccacatgcg ctggccacag
     4021 aggccggaat gaacgcctac cgggacggcg gcaatgcgat cgactcggcg atcgccgccg
     4081 ctgcggtgct cacggttgtg tatccgcaca acgtcgcgct cggaggcgat ctcatcgcgc
     4141 tggtgcgcac tccgaatggt gagactcatt gcgtcaacgc atccggctgg gcgggctccg
     4201 cggtcgacgt cgcgagcctg cgggccaggc acggaaggtg gctgcccgcg cgcggcgcgg
     4261 actcggtgac ggtgcccggt gggatccggg gatgggagac gttgcgtcgc ttcggcgctc
     4321 gattgacatg ggaccagacg ttgcgcgcag cggaatcgac cgcgcgggac ggcgcgccgg
     4381 tggctccgtc gctggcctct cacattgccg accccgagaa cgccgacttg ttcggcgtcg
     4441 aggacttcga ccgggtcttt cggcccgctg gtcgaaacct tcagaaggga gatgagttcg
     4501 cgcaaccagc gcttgcggac accttctcgg tgttgcgtga gcaggggccg gacgcgttct
     4561 acgaagggca actcgccgag cgctcgatcg catacctccg gtcttgcgga tcgtcattga
     4621 ccgtggagga tttcgccgct ttccaccccg acaccgtgga gccgatctcg gtgccgttcc
     4681 gcggtctgac ggtgctgacc agcccgccga acacccacgg cttcctgctg ctgcgggcac
     4741 tgcgtgccgt cgacgaactc ggcataaccg atccgctcga cgacggcctc ggtaccctgc
     4801 tgcgggtctt ccaccgaggc aacgccttac gttcgtcgta tttggccgat ccgcggcacg
     4861 ccagcgtcga cgtcgatgcg ctcgtcaatg atggtctgga cgaactggct gaactgggag
     4921 agatgccttc aggtccggcc ttggtgccgc acggcgacac agtcggcgtg gcggccgcag
     4981 atggtgacgg ctacgccgtg tcgctgattc aaagcgttta ccatgcattc ggctctggac
     5041 tgatcgatcc ggtcacaggc gttttgttcc acaatcgtgg gacgggcttc tcgctggacg
     5101 aggcctccct gaacgtcatc gcgccgcgta aacgccctgc gcacacgctg atgcccgtga
     5161 tgacgacaca ccaccggtca ccgagccacg tgctggccac gatgggcgga cagggccagc
     5221 cgcagatcct cgcccaggtg ttgttgcgag cggtcggtgg cgcgggcgcc gagtcggcgg
     5281 tcgctgcgcc gcgcgccatc gtcggactcc aagtcgacgg cgcgacgacc gattcggtga
     5341 ctgcggagtc cgacatcccg gcggcggcaa gggcctccat cggccgtgcg ggccttattg
     5401 cgactgacgt tgcggcgcac accgagtcac tcgggcagac caacgtcgtc ttcgctgacg
     5461 ccaagggatc gatgatggcg gcctcggatc cgcgttctga cggcgccgcc gtcgtggctc
     5521 attacccgcg attcgtccag ctctagtaga cgtcgggaac cttgacttgg gtttctggcg
     5581 tgagagtctc accgcggaag aagcgtttgg tgccgaacgc gaagcacaac agcatcagtg
     5641 ggacaccaag cgccaacatg ccgacaccga cgacgaacac gccgccgatg gggccgtatg
     5701 cggtgtagcc gtaatcgggt tcgatcatgt ccaccgcgct ctggacgaac gcccatgtca
     5761 tggccagcgc accgagcagc gggaagatgc cgcgcatgaa caggtttcgg gccgacgaga
     5821 acaggctgcg ccggaagtac cacacgcagg cgtatgcggt gatgccgtaa tagaacgcga
     5881 ccgccaggcc aagcgaggca atggaatcgg cgagtgcatt ctggctgacg aaggtcagca
     5941 cgagataaaa gcagagtgcc gtcacgccca tcacgatcgt gccgaacgcc ggcgtcatgt
     6001 atcggggatg cacgtaggcg aaccgcttcg ggatggcttc gtacaccgcc atcgacaagg
     6061 ttccgcgggc tgtcggcagg atcgttgtct gtgtggacga cagcgccgac accgacaccg
     6121 tcaacagaag cagcgccgca gcgaggctgc ccgcgaccgg ctcgccgagg atggtcagca
     6181 cgtcgtcgac gttctcctcg ttgttgaggc caatgtcttc gtcgccgaag cccgcgaacg
     6241 cctgcaccgc atacgcgacc agtacatacg tgccgagcag aatcagcgtg gtgatgactg
     6301 cggcccggcc gggcgtcttg tcggagcccg ggtctcctcg ccgacggcca aacacgtatc
     6361 ccagccccag tagatgaaga tgcacaagat caccgcttgc gctatcgacg acgcgtcgag
     6421 gcccgacggc cacagccagc tcagctgggg cgtgatggcc tggtcgccgg ccgatccgga
     6481 gaagacccgg gccaacgcga tgatgctgac aatgatgaga acgccgaatt ggattgcgat
     6541 gaggatgttt tggatgcgct cggacagcac gatgcctcgt gcgctaacga gtgacatcgc
     6601 gatgatgaag aaggagccga gcagtacttt tgcaggcagg ctctctgcga gcgaatccaa
     6661 gccgatgaag cggaacaaga agattgccgc gatctcggcg acgttggcca gcacgatgat
     6721 tgccgacacg gccagccccc agccaccgat ccagccgatc cagggaccga aagcctttgt
     6781 ggcccaagtg aatgtggtgc cgcagtcggg agtgtcgtgg gacagctccc ggtatgcgaa
     6841 ggccaccagg agcattggga tgaacgcgag cacgaacatc gacggtgact tctcaccgac
     6901 cgcggcacga cgtagcccag cgtggcagcg aggctgtatg ccggtgcgac ggacgcaagt
     6961 ccgacgacga cgttgccgac cagtccgagc gcgtcggcct gcagcccctt gtcgctggtg
     7021 acgtgagtgg gaggttcgct gactgccatg tgcggaatat acggccgacc ggcggcccat
     7081 atgggcaaac taaactcggc acatgaccgc gagtacgcgt gtcgacgagt tcgaggagct
     7141 gcggccgcat ctgctggccg tcgcgtatcg gctcacgggc acggtcgccg actccgagga
     7201 catcgtgcag gatgcctggc tgcggtggga cggcacgtca gagtccatcg tcgatctgcg
     7261 ggcgtggctg acgacggtgg tcagcaggct gagcctggac cggctgcgct cggcggccaa
     7321 gcgtcgcgaa acctatgtcg gcgaatggct gcccgaaccg gtggtcacgg gcttcgacag
     7381 caacgacccg ctggcggcgg tggtggccgc cgacgacgcc cggttcgcgg caatggtggt
     7441 gttggagcgg ttgacgccgg atcagcgagt ggcgttcgtc ttgcacgacg ggttcgcggt
     7501 gcccttcggt gagatcgcct ccgtgctcgg cgtcagcgag gcctcggccc ggcaactcgc
     7561 gtcgcgcgca cggcgcgctg ttgcgtcggc gccgccagca gtggccgacg acacccacaa
     7621 cgaggtggcc ggctcgctga tggcagccct tgccgagggc gacatgaaag ccgttgtgcg
     7681 cctgctgcat cccgatgtca cgttcaccgg cgatgccaac cgcagggcgc cgacggccgc
     7741 cagggtcatc cgcgggccgg acaaggtggc gcggttcctg ttcgggttgg cccgtcggta
     7801 cgggccggga tggctggcgg cgaaccagct ggcgctggtc aacggacagc tgggc
//
LOCUS       DASRJA010000131         3758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2719649,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000131 DASRJA010000000
VERSION     DASRJA010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2719649"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..807)
                     /locus_tag="VFC01_05960"
     CDS             complement(94..807)
                     /locus_tag="VFC01_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HZN79221.1"
                     /translation="MTEPVKITNPVRLDDLINAIKKVHDEPLDQLTDAVIAADALGDI
                     ADHLIGHFVDQARKSGASWTEIGKCMGVTKQAAQKRFVSKADAALDPEAGFGRFTPRA
                     RNVIVVAQNKAHGAGNAEIGSDHLLLALFGEGEGLAVKLLAGQGIDADKVNKAVTLPP
                     RVDDIPVLIPFNGAAQKVLELTFRQALRLGHNYVGTEHILLALYEAEDDDGTLHQLGV
                     DRDRFERELVAALETYTKT"
     gene            complement(859..1335)
                     /locus_tag="VFC01_05965"
     CDS             complement(859..1335)
                     /locus_tag="VFC01_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN79222.1"
                     /translation="MTERIEVQRTIAAPPADIFAVLCDPQGHVAIDASGMLQDADGDP
                     AQAVGDTFVVHMDREALNDYPLGKYDVTVTIREFDKDRLIAWTILGQIRPQIGHIYGY
                     RLEPADGGTLVTSFYDWSDIDPHWREANIFPVVPESALRATLGILDRTVRRGYPRG"
     gene            1411..1773
                     /locus_tag="VFC01_05970"
     CDS             1411..1773
                     /locus_tag="VFC01_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN79223.1"
                     /translation="MPNGVETSAAQPQTLRRAIAFIHDNAHRDIGVNDIAAAINVTPR
                     SVQYTFRRHLGTTPLEYLRRVRLDRAHRDLQAADPAVDTVMEIAGRWRFGHPGRFSIA
                     YKEAFGQPPSLTLRRNAG"
     gene            complement(1789..2217)
                     /locus_tag="VFC01_05975"
     CDS             complement(1789..2217)
                     /locus_tag="VFC01_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79224.1"
                     /translation="MIRLLFAAVAIAGAAVCTAPAALADPGSDNLYFDRPGRYATDVP
                     GMNYDAHLAGPCDNMDFFTFGRGPGGEALQCRWIANQWPPVTTGFWVATYELFGVQEI
                     GTPCPKPQAAAQSPDGRPLLCLGADGWQPGFFTREGFFQG"
     gene            complement(2374..2749)
                     /gene="tnpA"
                     /locus_tag="VFC01_05980"
                     /pseudo
     CDS             complement(2374..2749)
                     /gene="tnpA"
                     /locus_tag="VFC01_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            2817..2918
                     /locus_tag="VFC01_05985"
     CDS             2817..2918
                     /locus_tag="VFC01_05985"
                     /inference="COORDINATES: protein motif:HMM:NF023742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79225.1"
                     /translation="MVMRYRYRIEPTSAQQAMLARVFGCCRVWPDPD"
     gene            2981..3758
                     /locus_tag="VFC01_05990"
                     /pseudo
     CDS             2981..3758
                     /locus_tag="VFC01_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611217.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          629 a         1131 c         1333 g          665 t
ORIGIN      
        1 cccgcagcac ggtcgcgccg tgctcggcca ccacatcctc gaacggtcgc acctccacat
       61 catgaagacg cgagaccggc agcggatgtg agatcaggtt ttggtgtacg tctcgagggc
      121 cgcgacgagc tcgcgctcga agcgatccct gtcgacaccc agctggtgca gcgtgccgtc
      181 gtcgtcttcg gcttcataca gcgccagcaa gatgtgttcc gtgccgacgt aattgtggcc
      241 cagccgaagt gcttggcgga aggtcagttc cagcaccttc tgggccgctc cgttgaaggg
      301 gatcaggacg ggtatgtcgt cgactcgcgg gggcagcgtc accgccttgt tcaccttgtc
      361 ggcatcgatg ccctgaccgg cgagcagctt gaccgcgagc ccctcgccct cgccgaacag
      421 cgccagcagc agatggtcgg agccaatctc ggcgttgccc gcgccgtggg ccttgttctg
      481 ggcgacgacg atgacgttgc gggcgcgagg ggtgaatcgg ccgaagcccg cctccgggtc
      541 gagggcggcg tcggccttgg acacgaaccg tttctgcgcg gcctgcttgg tgacgcccat
      601 gcacttgccg atctcggtcc acgacgcccc ggacttgcga gcctgatcca cgaagtggcc
      661 gatcaggtgg tcggcgatat cgccgagcgc gtccgccgcg atcacggcgt cggtcagctg
      721 gtcgagcggc tcgtcgtgga ccttcttgat ggcgttgatc aggtcgtcga ggcggacggg
      781 gttggtgatt ttgacgggct cggtcatgcc gtcaactcta ggttgacgct gttggttcgt
      841 caaccttggg ttgacgcctt agccgcgggg atatccgcgg cgcacggtgc ggtcgaggat
      901 gcccagggtg gcgcgcagcg cgctctcggg tacgacggga aagatgttgg cctcgcgcca
      961 gtgcgggtcg atgtcggacc agtcgtaaaa cgacgtgacc agcgtgccgc cgtcggcggg
     1021 ttcgagccga tagccgtaga tgtggccgat ctgcggccgg atctggccga gaatcgtcca
     1081 tgcgatcagg cggtccttgt cgaactcacg gatcgtgacg gtgacgtcgt acttgcccag
     1141 tgggtagtcg ttgagcgcct cgcggtccat gtgcacaacg aaggtgtcac caacggcctg
     1201 cgcggggtcg ccgtcggcgt cctgcagcat gccggatgcg tcgatggcta cgtggccctg
     1261 cgggtcgcac aggaccgcga agatgtcggc gggtggtgcg gcgatggtgc gttggacttc
     1321 gatgcgttcg gtcatgggca gatcgtgtca ggacggcgat cggcctaggg gcgagtttgg
     1381 gatctcggct gccgggtaat cgacgggcta atgccgaacg gtgtcgaaac gtctgccgcg
     1441 caacctcaga cgttgcgccg ggcaatcgcg ttcattcacg acaacgccca ccgagatatc
     1501 ggggtgaacg acatcgccgc ggcgatcaat gtcaccccgc gctcggtgca gtacacgttt
     1561 cgtcgccacc tcggaacaac gccgctggag tacctgcgtc gggtgcgcct cgaccgcgcg
     1621 caccgcgatc tgcaggccgc cgatccggcc gtcgacacgg tgatggagat cgccggccga
     1681 tggcggttcg gtcatccggg ccggttcagc attgcgtaca aggaagcgtt cgggcagccg
     1741 ccaagtctga cgctccggcg taacgccgga taacccctag ggcagcggtc agccctggaa
     1801 gaaaccttca cgggtgaaga acccgggctg ccagccgtct gcgccaaggc acagcagcgg
     1861 acgcccgtcg ggggactggg ccgccgcttg tggcttcggg cagggggtcc cgatttcttg
     1921 aacgccgaac aactcgtagg tcgccaccca gaagccggtg gtcactggag gccactggtt
     1981 cgcgatccac cggcattgga gggcttcacc gccggggccg cgaccgaagg tgaagaagtc
     2041 catgttgtcg cacggcccgg cgaggtgggc gtcgtagttc atgccgggca cgtcggtggc
     2101 gtagcgcccg ggcctgtcga agtacaggtt gtcgctgccg gggtcggcta gcgcggccgg
     2161 cgccgtgcag acagcggcgc ccgcgatcgc aacggcggcg aaaagtaggc gaatcatgtc
     2221 ccaccccttg gtcgtcgtga ccggttggtc tctgcgaagc ctaacgggtg cggtgtggtc
     2281 ggtgaacgtt ttactgacca agttcttggg cgcaagcctc cgacttcagt ccgggtgagc
     2341 ccatcccgtg tgccgccgga ggtagccggg cgttcagagt ggtcgggctt gtccgtcgat
     2401 gtattgcttg atgatggtca ggggtgcacc gcgcaggaga cggcgaagta ggacggggac
     2461 cagaggtgct tcgcatgccg gcgcggacac cgacgcggtg aattcgcggt acagcgcgta
     2521 ggcgctgcga cttttgagtc ggtgcgccag tacggggatc gccagcgtcg gagggtaggc
     2581 cacgagcagg tgcacgtggt cggcctggcc gttgaactcg acgaactcga catcgagttc
     2641 ggcgcacaca ctgcgcaggg cgtgctcggg agaaggtgag catcgcattg gtgaacactt
     2701 cgcgtcggta tttggtcacg aacaccagat gggcgtgggc cagcgccacg ctgtgttggg
     2761 cgcggcggtc ggtctgggct gtctttgttg tcgtaggtgg gctttagggt cgggtcatgg
     2821 tgatgcgcta ccggtatcgc atcgaaccga cttcggctca gcaggcgatg ctggcgcggg
     2881 tgttcgggtg ctgccgggtg tggcctgacc ccgattgatt gatccgtggc ttatgagagt
     2941 cttgcggccc acgacgtggg caggaaggct caacaagaac atggcgacga ggaagcggca
     3001 cagtcccgag cagatcgtgc gcaagttgac ggcggctgat cggctactag ctgagggcaa
     3061 ggacaccgcg gcggtgtgcc gggagctggg cgtgtcggag gcgacctatc atcgctggcg
     3121 taaccaattc ggcgggttga aggccgagga cgccagacgg ctcaaggacc tagaacgcga
     3181 gaactccacc ctcaagcggc ttttggcgga gtccgagctg gagaaggcgg cgctcaagga
     3241 gatcgcgaag ggaaacttct aagcccggaa cgccggcggg cagccgttcg tcatctccag
     3301 cgtgtgctgg gggtcagcga gcgattcgcc tgccgcgtga ccgggcaaca ccgcgccacc
     3361 caacgacacg aacccgccac cgataccccc gacgatcccg acgccgcctt gcgggactgg
     3421 ctgcgcacct acgccaagga tcatccgcgc cgtgggttcc ggcccgccta tcatgacgcc
     3481 cgcgccgaag gctggatcgt caatcacaag aagatccaac ggctttggcg tgaggaaggg
     3541 cttcgggtgc cacagcggcg acgccgaaaa cgccgcggca cctctaccgc agcgccggcg
     3601 gtcgtcgcgg atgcgcccaa tcgggtgtgg gcggtggact tccagttcga ttccaccaca
     3661 gacggacggc cgatcaagat cgtctcgatc atcgatgagc acacccgcga atgcttgggt
     3721 gggatggtcg agcgcggtat cactggcgag cacctgag
//
LOCUS       DASRJA010000132        11411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1591023,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000132 DASRJA010000000
VERSION     DASRJA010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591023"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /gene="thiG"
                     /locus_tag="VFC01_05995"
     CDS             complement(<1..313)
                     /gene="thiG"
                     /locus_tag="VFC01_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214931.1"
                     /note="functions in thiamine (vitamin B1) biosynthesis; in
                     Bacillus subtilis this enzyme catalyzes the formation of
                     thiazole from dehydroxyglycine and
                     1-deoxy-D-xylulose-5-phosphate and ThiS-thiocarboxylate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HZN79226.1"
                     /translation="MADSKLTIAGREFGSRLIMGTGGAANLAVLEEALIASGTELTTV
                     AMRRVDADNGTGVLDLLNRLGISPLPNTAGCRGAAEAVLTAQLAREALGTDWVKLEVV
                     AD"
     gene            complement(306..503)
                     /gene="thiS"
                     /locus_tag="VFC01_06000"
     CDS             complement(306..503)
                     /gene="thiS"
                     /locus_tag="VFC01_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HZN79227.1"
                     /translation="MFVSINDDIVEVDEQTTVAALLDGLCLPDKGIAVAVNWAVIPRS
                     DWHVPLQQGAQVEVFSAAQGG"
     gene            754..1149
                     /locus_tag="VFC01_06005"
     CDS             754..1149
                     /locus_tag="VFC01_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HZN79228.1"
                     /translation="MPIYTCTMAESTFGADAKRALASEIARIHSSMIHVPSTYVNVAF
                     HELDADGLYAGGVPASPVLVTGWVREGHPADDITRLATEIATAVTKTLEIDVERVLVV
                     IQPSPARFAVEGGRVLPEPGQEKEWLAGS"
     gene            1208..2386
                     /locus_tag="VFC01_06010"
     CDS             1208..2386
                     /locus_tag="VFC01_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HZN79229.1"
                     /translation="MPSNFGDFQIGVYLDAVKGVNSRYPFDSQSIERKASEALPDWVY
                     RYVSSAAGDGRTQRANIAAYSRYGITPRMMVSPPERDLSITLFDKHFDSPIFMCPIGL
                     IGLCAPDFQGDVAAAQASAATGVPFTLSTFSQAPMEDVIKHAGTTPNFYQLYLPGDRE
                     LAASLINRAETSGYSALVLTVDTWALGYRPADLQLGNFPQFRGFCMENYYSDENFTKH
                     LAKPPHEDQAAALQHYGKIFQHPVSWEDLRWIRSQTNLPIAVKGIQHPDDARMAIDNG
                     ADVVYCTNHGGRQANSGIPTLELLPGIVAAAGSTPVMFDSGVRDATDAVIALARGATA
                     VGIGRPYAYAVSYGGAESLTHFLHSFLAELDLCLAICGFKDIASLKEAGCEPTMDLQN
                     "
     gene            complement(2412..3245)
                     /locus_tag="VFC01_06015"
     CDS             complement(2412..3245)
                     /locus_tag="VFC01_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007317794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN79230.1"
                     /translation="MTMVAVVTGASRGAGKGIAVALGRTGATVYVTGRSRNADDSPYG
                     GTVGETAALVTEAGGRGVPMAVDHGDDEAVAELFATVGREQGELDILVNNAAKATVVR
                     GPFWTEPPAVADLLTVGLRSHYIAAFHAAPLMIAGGRGLIVHTGHYGAVSYHQGPAYG
                     AQKAGADKMAADMARELRPHHVAAVSIWMGGLDTERVRAHADALPPETRDVRRESPEF
                     TGRVIAALYRSPELMTLSGRTLIGAELGAHLSVTDVDGSVPISYRDTLGAPPEPHVSL
                     R"
     gene            3322..3636
                     /locus_tag="VFC01_06020"
     CDS             3322..3636
                     /locus_tag="VFC01_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79231.1"
                     /translation="MARNLVHADPATVTLQEALDALADPVRRSILRELSTEPNFTRPC
                     GSFDLPVSKATASHHFAVLRATGLLEQADRGPRRFNRLRREEFDARFPGLLALVLAEA
                     QA"
     gene            3689..6412
                     /locus_tag="VFC01_06025"
     CDS             3689..6412
                     /locus_tag="VFC01_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN79232.1"
                     /translation="MELIDRHAERGVLDGLLEAIRVGESRALVVSGEAGVGKTALLEY
                     LSAQASDCHLARAAGVQSEMELPFAGLHQMCAPVLGNLPRLPPPQRDALRIAFGMSAG
                     SAPDRFLVGLAVLGLLSATAEQRPLVCVVDDEQWLDRTSAQVLGFVARRLMAESVALI
                     FAARVPSGELGGLPGLVVGPLAEVDARALLDRVLSGPLDSRVRDRILAETQGNPLALL
                     ELPRGLTPQQLAGGFGLLGAVRLSRGIEENFRHRIEALPVQTRRLLLIAAAEPLGDPA
                     LLWRAGALMGIDADAAVPAVEANLIEFGSRVQFRHPLVRSVLYGSAPPRERREVHAAL
                     GDATDPQQDPDRRAWHRAEAAPGPDEEVAEELERSAGRARARGGLPAAAAFLERATVL
                     TLDSTRRTERALAAASATIKSGAFDAARDLLGVAESGSPDDFQQARIDLMEAELALLT
                     SRGSDAPSLLLKAARRLEPIDADLSRATYLQALASGYFAGRLATGGGVLEVARAAATA
                     PPPTHTQRAPDLLLDGLVAHYNVGYAAGLPIIRKGLKAFGTGMAVDEELRWHWVAGIM
                     ARHIWDDESWHRLSDRHVQLARSVGALSELPVALNSRAFMLLFAGELPAAAALIQELQ
                     PAIEATGTRLAPYPTLGLAVLAGRTAEATALIDAITRDVTMRGEGIGLTVVDWAYAVL
                     NNGLGDYQEAMTAAQRSTGYLREMTAPTWTMVELIEAAARSGHDDIAADALARLAETT
                     SASGTDWGLGVEARSRALVSEGDTAERLYREAIERLARRRVRADLARAHLVYGEWLRR
                     ERRRVDARAELRIALEMLESIGMDAFAERARRELQATGETVRKRTITTNDHGLTAQES
                     QVARLARDGLSNPEIGARLFISSRTVQYHLRKVFAKLGINSRSQLGRVLPD"
     gene            complement(6422..6982)
                     /locus_tag="VFC01_06030"
     CDS             complement(6422..6982)
                     /locus_tag="VFC01_06030"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZN79233.1"
                     /translation="MASPQIPNAFADFMNVLVLVGLPEGPINRALGRHAIDSSPEGVM
                     VNVINDLARLPRYREALESQGKPDAVVDLRLAAAEADAVLVLTSYRGRVPSIAHNAID
                     WLTHRWRRDAMHRKPLAVVGRSSGYYSGVLSRRIADTRGGLGPRVIEPLTARTLHEAV
                     KKLADEVSGHPTVVRTGAFEAEWLPH"
     gene            complement(7185..7781)
                     /locus_tag="VFC01_06035"
     CDS             complement(7185..7781)
                     /locus_tag="VFC01_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79234.1"
                     /translation="MQRIDYVHGWWLLRACRRNPLVRGTDRLELLIIALGIFVVLVSA
                     ACAGALGTAVHDARSRVYVAQAQTRHTVTARATEDSTIVLRVDDSTATRVNARWQDNG
                     SEHTGSLDWDHAVKTGDPLTIWVDRNGDRVEAPARTSQAGADAVGVAYAVWQTVALAV
                     AGFVCWGRRRLDRRRDSAWERDIRCLIDDGGGRTNPKS"
     gene            complement(7809..8486)
                     /locus_tag="VFC01_06040"
     CDS             complement(7809..8486)
                     /locus_tag="VFC01_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HZN79235.1"
                     /translation="MSETRPQATTTPAGAVGRFFDEAVSKSRFWWLLLITGVAWIVLS
                     IVILRFNYTTVAAVAVLFGVYCLVAAVNETMIGALSSSTGWRIAHGLLAALLVVAGVV
                     SLANFGATFVTLAAIISFYFIFRGCFDVVMAFAANMVSGWWVLLISGLIELGLGFWAA
                     GSWNNSVVVLVAWVAAAALVHGVGEIALAFQIHQGRRGITAVEGLADRLGVTNNHPAP
                     DTAVPTS"
     gene            8640..8960
                     /locus_tag="VFC01_06045"
     CDS             8640..8960
                     /locus_tag="VFC01_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79236.1"
                     /translation="MTVANRRWQMVARRTMTARMRALTIGGSRNCNHEGASMSTLSKL
                     HESAAKHHEHAARHHREAAKLHEVKDVLAAVDQAHMATDHQVHAIHYATQAAKEYLAA
                     HRGS"
     gene            complement(9074..11167)
                     /gene="glgX"
                     /locus_tag="VFC01_06050"
     CDS             complement(9074..11167)
                     /gene="glgX"
                     /locus_tag="VFC01_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007237708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HZN79237.1"
                     /translation="MSATVLPGRPAPLGATPGPGGTNFAVASNGDEVRLCLFSADGSE
                     TQLPLPERDGDVWHGFVPGVAPGQAYGYRVSGSYNRPRGQRYNPAKLLLDPYARAIHG
                     DVRFGPELLDYATDNSGAPSPLDSAGQMPRSLVTAPAFAPLPLGPRHALADTILYEVH
                     VRGFTAKHPGVPQELRGTYAGLAHEAAVRHLVDLGVTTVELLPIHHNVPESFLVERGL
                     TNYWGYNTIGFFAPHAAYSAAVRAGQPGGQVEEFRNMVAALHAAGLEVVLDVVFNHTA
                     EGGAGGPTLCHRGLDNAAYYRLEPSDLNRYVDTTGCGNSLNTADATTLRMIMDSLRYW
                     VSEMGVDGFRFDLAPTLGREDGDFDPFSAFFDTVGQDPVVSQVKLIAEPWDVGRMDSY
                     DIGRFPPLWSEWNGRFRDTVRDWWRSHDRLLPDFATRLCGSADIYDRPDEARRPSASV
                     NFVTVHDGFTLHDLVSYDRKHNEANTEDNRDGTDDNRSWNCGAEGPTGDTGVNAVRAR
                     QKRAFLVTLLLSAGVPLLLGGDELGRTQRGNNNAYCQDNEITWFDWSAVDEDLLRFTQ
                     ELIALRRRHPVFRRRRYSTGKEAADLRWFTPAGTEMTTENWADPNALCVALFIDGSTD
                     PDVGVDGTPMVDNDFLVLINAWWEPLAFAVPGNLPARRWDVVCDTFDPARKGTVAQEL
                     TVGPRSIVILQSPPS"
     gene            complement(11164..>11411)
                     /locus_tag="VFC01_06055"
     CDS             complement(11164..>11411)
                     /locus_tag="VFC01_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79238.1"
                     /translation="LVGHDGRRPCFGGVQRMQTDPRWRDNLLFHEYFHGDTGAGLGAS
                     HQTGWTGLVADVIRRRHHAYPKASEVIQRLVQGEETA"
BASE COUNT         2010 a         3743 c         3737 g         1921 t
ORIGIN      
        1 cgtcggcgac cacctcgagc ttgacccagt cggtgcccag cgcctcacgc gccagctgtg
       61 ctgtcaacac cgcctcggcc gcaccacggc agcccgcggt gttgggcagc ggactgatgc
      121 ccagccggtt cagcaggtca agcacgccgg tcccgttgtc cgcgtcgacg cggcgcatcg
      181 ccaccgtggt caactcggtg cccgacgcga tcagcgcctc ctcgagcacg gcgagattcg
      241 ccgctccgcc ggtgcccatg atcagccgcg agccgaactc gcgaccggca atcgtcagtt
      301 tcgaatcagc caccctgcgc cgccgaaaac acttccacct gcgcgccttg ctgtaggggg
      361 acgtgccaat ccgatcgcgg aatcaccgcc caattgaccg cgaccgcgat ccccttgtcc
      421 ggcaagcaaa ggccgtcgag cagcgccgcg acggtcgtct gctcatcgac ctcaacaata
      481 tcgtcgttga tgctcacaaa catctgcatc ctcccgcggt agccggctca cgctccgagc
      541 gtggcgtagg actcgaccga cgtcgatgtg cccgcacaca acgtccacag cgaaagcctg
      601 tgagatgcca aacgtggaaa gcgctgcagc ggctgtggtt cagcacatcg gcaaaccaag
      661 atagttgtcg ccgaggacag cgactccgcc cgaggagatc gcgacgatga attgcagcca
      721 ccccgacctg acctgaatga aggagcagca acgatgccga tctacacctg caccatggcc
      781 gaatcgacct ttggcgccga cgcgaaacgc gcattggcta gtgagatcgc ccgcattcat
      841 tcatcgatga tccacgtgcc gagcacctat gtgaatgtcg cctttcacga gttggacgcc
      901 gacggtctgt acgccggcgg cgtgcctgcg agccctgtgc tggtcacggg ttgggtgcgt
      961 gaaggccacc ccgcggacga catcacaagg cttgccaccg agatcgccac cgctgttacc
     1021 aagacgcttg agatcgatgt ggagagagtc ctcgtcgtca ttcaacccag tccggctcgc
     1081 ttcgccgtgg aaggcggacg tgtgctgccg gagccgggcc aagaaaaaga gtggctcgcc
     1141 ggaagttgat ggcaagcggt agcacgcgtc gccagaccga tttcgaacca cgtcaaggag
     1201 ttacgccatg ccaagcaatt tcggtgactt ccagatcggc gtctatctgg acgccgtgaa
     1261 gggcgtcaac tcgcgatacc cgttcgactc tcagtccatc gagaggaagg cgtccgaggc
     1321 gcttcctgac tgggtgtacc ggtatgtctc ctcggcagcc ggagacggcc ggacccagcg
     1381 cgccaacatc gccgcatact cacgctacgg aatcactcct cggatgatgg tcagcccgcc
     1441 cgagcgtgac ctgtcgatca cgttgttcga caaacatttt gactccccga tcttcatgtg
     1501 ccccatcggc ttgatcggct tgtgcgcgcc tgacttccag ggagatgtgg ccgcagcgca
     1561 ggcgtcggcc gccaccggtg tgccctttac cctgtcgacg ttctcccagg ccccgatgga
     1621 ggatgtcatc aaacacgccg ggaccacgcc gaacttctac cagctgtatc tgccaggcga
     1681 tcgtgagctg gcagccagcc tcatcaatcg tgctgaaacc agcggatatt ccgcgttggt
     1741 gttgacggtc gacacgtggg ctctcggcta ccgacctgct gacctgcagc tgggcaactt
     1801 cccgcagttc cgcggcttct gcatggagaa ctactactcc gacgagaatt tcaccaaaca
     1861 cctcgcaaag ccgccgcacg aggatcaggc cgcagcgttg cagcactacg ggaagatttt
     1921 tcagcacccg gtgtcgtggg aagacctccg ttggattcgg tcgcagacca atctgccgat
     1981 cgcggtgaag gggattcagc accccgatga tgcccggatg gcgatcgaca atggcgccga
     2041 tgtcgtgtac tgcaccaatc atggcggtcg ccaggcgaat tcgggaattc caacactgga
     2101 gctcttgccc ggcatcgtgg cggcggccgg ctcgacgcct gtcatgttcg actccggcgt
     2161 gcgggacgcg acggatgccg tgatcgcact ggcccgcggc gccacggccg tcggcatcgg
     2221 caggccctac gcatacgccg tgtcctatgg cggcgccgaa agcctcaccc acttcctgca
     2281 ctcattcctc gccgaactgg acctgtgctt ggcgatctgt ggcttcaagg acatcgccag
     2341 cttgaaagaa gcgggctgcg aaccgaccat ggatttgcag aactaaatcc gaacctcctg
     2401 ctgatgcacc gtcaccgcaa gctgacgtgc ggttccggcg gagcgcccag ggtatcgcgg
     2461 taggagatcg ggacactgcc gtcgacatcg gtgacactca ggtgtgcgcc cagttccgcg
     2521 ccgatcagcg tgcgtccgga gagcgtcatc agttcgggtg accggtacag cgcggcgatc
     2581 acccggccgg tgaactcggg cgactcgcgc cgcacgtcac gcgtctccgg cggcagggca
     2641 tccgcgtggg cgcgcacgcg ttcggtgtcc agcccgccca tccagatcga gacggcggcg
     2701 acgtggtgcg ggcgcagttc cctggccata tcagcagcca tcttgtcggc tcccgccttc
     2761 tgcgccccgt aggcgggccc ctggtggtag gacaccgcgc cgtagtggcc ggtgtgcacg
     2821 atcagcccgc ggccgcccgc gatcatcagt ggcgcggcgt ggaaagcggc gatgtagtgg
     2881 gagcgcagcc cgacggtgag cagatccgcg accgccgggg gctccgtcca gaacggaccg
     2941 cggacgacgg tggccttggc cgcgttgttg acgaggatgt cgagctcgcc ctgctcgcgg
     3001 ccgaccgtgg cgaacagctc cgcgaccgct tcgtcatcgc cgtggtcgac ggccatcggc
     3061 acgccccggc cgccggcttc ggtgaccagt gcggcggttt cgccgaccgt gccgccgtaa
     3121 ggcgaatcgt ccgcgtttcg gctgcggccg gtcacgtaca ccgtcgcgcc ggtccggccc
     3181 agcgccaccg ctatgccctt gcccgcgccg cggctggccc cggtcactac cgccaccatc
     3241 gtcatggcgt gcctccttgt gttagatgct tatcgaacat acgccagtgt acgatcacca
     3301 tcgaacagag gagggcgcgg agtggcgagg aacctggtgc acgccgatcc ggcgacggtg
     3361 accttgcagg aggcgctcga cgcgttggcc gatccggtac ggcggtcgat cctgcgggag
     3421 ctgtcgaccg aaccgaattt cacccggccc tgcgggtcgt tcgacctgcc cgtgtcgaaa
     3481 gcgaccgcca gccaccactt cgccgtgctc cgcgccaccg ggctgctgga gcaggccgac
     3541 cggggcccgc gccggttcaa ccggttgcgg cgcgaggagt tcgacgcgcg tttccccggc
     3601 ctgctcgcgc tcgtgctggc cgaggcgcag gcctgacccg tggttagcgc cactgccgtg
     3661 cagcggacct cacgggtgca ggcgcgcgat ggaactgatt gaccgccacg ccgagcgcgg
     3721 cgtgctcgac ggactgctcg aggcgattcg cgttggcgag agtcgggcgt tggtggtttc
     3781 cggtgaagcg ggtgtgggca agacagcgct gctcgagtat ctgagcgcgc aggcgtcgga
     3841 ctgccatttg gcgcgtgccg ctggtgtgca gtcggaaatg gaactgccgt tcgccggact
     3901 gcatcagatg tgcgcgccgg tgctcggtaa cctcccccgc ttgccaccac cgcaacgaga
     3961 cgcgcttcgg attgcatttg gcatgagtgc cggatcggca ccagaccgct tcctggtcgg
     4021 actcgcggtg ctgggcttgc tgtccgctac cgccgagcaa cgaccgctcg tgtgcgtcgt
     4081 agatgacgaa cagtggctcg accgcacctc ggcgcaagtg ctcggatttg tcgcgcgcag
     4141 gttgatggcg gaatcggttg cgttgatttt cgcggctcgc gtaccgagcg gcgaactcgg
     4201 gggcctgccc ggtctggtgg ttggcccgct cgcggaggtc gacgcgcgtg cgttgttgga
     4261 tcgcgtgctg tcgggaccgt tggacagccg cgtgcgggac cggatcctgg ccgaaactca
     4321 aggtaatccg ctagctctgc tggagttgcc tcgcggattg acgccgcaac agctcgcggg
     4381 tgggttcggg cttcttggcg ccgtgcggtt gtcccgcggt atcgaagaga acttccggca
     4441 ccgaatcgaa gcgctcccgg ttcagacccg tcgactcttg ctcatcgcag cagctgagcc
     4501 cctgggtgat ccggccctgt tgtggcgagc cggtgcgctg atggggatcg acgccgacgc
     4561 cgcggtgccc gcggtcgagg ccaacctgat cgagttcggt tctcgggtgc agtttcggca
     4621 tcccctcgtc cgctcggtgc tctacgggtc ggcgccgccg cgggaacggc gtgaagtgca
     4681 cgctgccctt ggcgacgcca cggatccgca acaagatcca gatcggcgcg cgtggcaccg
     4741 ggcggaagcc gcccctggac ccgacgagga ggtcgccgag gagttggagc ggtcggcggg
     4801 gcgcgcgcgt gcccgaggcg gcctgcccgc cgcggccgca ttcctcgaac gcgcgacagt
     4861 gttgaccctc gactccacga gacgcaccga acgcgcgctg gcggcggcgt cggcgacgat
     4921 caagtcgggt gcgttcgacg ctgcacgcga tttgttgggc gtcgcggagt cgggatcacc
     4981 agacgacttt caacaagccc ggatcgacct gatggaggcg gaactcgcgc tcctcacaag
     5041 ccgaggaagc gatgcgccgt ctctgctgtt gaaggccgcc aggcggctcg agccgatcga
     5101 cgcagacctc tcccgggcga cctatctgca ggctctcgcg tcgggctact tcgccggtcg
     5161 cctcgcgacg ggcgggggtg tcctggaagt ggcacgcgcc gcggcaaccg cacccccgcc
     5221 gacgcacact cagcgcgcac ccgaccttct gttggatgga cttgtcgcac actacaacgt
     5281 ggggtatgcg gctggactgc ccataatccg aaaggggctc aaggcgtttg gcaccggcat
     5341 ggcggtcgac gaggagctgc gttggcactg ggtggcgggc atcatggccc gccacatctg
     5401 ggacgacgag agctggcaca ggctctcgga tcgccatgtg cagctggccc gctccgttgg
     5461 ggcgctcagc gagctgccgg tcgcactcaa ttcgcgagcg ttcatgctgc ttttcgccgg
     5521 cgagctgccg gccgcggcag cgctgattca ggagctgcaa ccggcgatcg aggcgaccgg
     5581 caccaggctc gcgccctacc ccaccctcgg cctcgccgtg ctagccggca ggacggcgga
     5641 agcgaccgct ctgatcgatg cgatcacaag ggacgtgacc atgcgcgggg aagggatcgg
     5701 gctgaccgtg gtggattggg cgtacgcagt gctgaacaac gggctcggcg actaccagga
     5761 agcgatgacc gcagcccaac ggtcgaccgg ttacctgcgg gagatgacgg ccccgacgtg
     5821 gacgatggtc gagttgatcg aggcggccgc gcgcagcgga cacgacgata tcgccgccga
     5881 cgccctcgct cgactggccg agacaaccag cgccagcggc accgactggg ggctcggtgt
     5941 cgaggcgcgt tcgcgggcac ttgtgagcga aggggacacc gcggagcgtc tctatcgtga
     6001 ggcgatcgaa cgtctcgccc gccgccgggt ccgggcagac ctggcccggg cacacctggt
     6061 ctacggggag tggctgcgtc gggagcggcg gcgggtcgat gccagggctg agctccgtat
     6121 cgctctcgag atgctggagt cgatcgggat ggacgcgttc gccgaacggg cgcggcgtga
     6181 actgcaggcc accggcgaga cggtccgcaa gcgcaccata acgacaaacg atcatggact
     6241 caccgcgcaa gagtcgcagg tcgcgcggtt ggcgcgggat ggcctgtcga atccggagat
     6301 tggcgcccgg ttgttcatca gctcgcgcac cgtccagtac cacctccgca aggtcttcgc
     6361 caagctcggc atcaattcgc gcagccaact cgggcgggtt ctccccgatt gaccgtgaag
     6421 ttcagtgcgg cagccactca gcttcaaatg cacctgttcg tacgacggtc gggtgaccgg
     6481 acacttcgtc ggcgagcttc ttgaccgctt cgtgcagagt gcgcgcggtc agcggctcga
     6541 tcactcgggg tcccaaacct ccacgggtgt ctgcgattcg acgcgacaag acgccgctgt
     6601 agtacccgga cgagcggccg acgacggcga gcggcttgcg atgcattgca tcgcgacgcc
     6661 agcgatgtgt cagccagtcg attgcgttgt gcgctatcga gggaactcgc ccgcggtagc
     6721 tcgtgaggac gagcaccgca tcagcctcag ctgcggccaa ccgcaagtcg acgacagcgt
     6781 ccggcttccc ttgggattcc aaggcttcgc ggtagcgggg caggcgggca agatcgttga
     6841 tcacgttcac cattacacct tcaggtgagc tgtcgatcgc atgtcggccc aacgcacggt
     6901 tgatcggacc ctcggggaga cccaccagga ccaacacgtt catgaagtcc gcaaatgcat
     6961 ttggtatttg tggagacgcc atcctcgtta ccttgtccgt atcgatgtga agcgttcgca
     7021 ggcgctgccc ggcgattact tgtgacaaac cgatcgtccg atgggccgca acggctgtcg
     7081 atggtgtcgc cgacaagtcc gcgctaccgc cgatgtcggt gtgcacaccg gaggagacca
     7141 actgtggacg acgacatcga ctgccgcaca ggcacggtgc gcagtcatga tttcgggttt
     7201 gtccgcccgc cgccgtcatc gatcaggcat cggatatcgc gttcccaagc cgaatcgcgc
     7261 cggcggtcaa ggcgcctgcg gccccagcag acgaatccgg ccacagcaag ggccactgtt
     7321 tgccatacgg cgtaggcgac gccgaccgcg tccgcgccgg cctgcgatgt ccgcgcgggc
     7381 gcttcgacgc gatcgccatt acggtccacc caaatcgtga gcggatcacc ggttttgacg
     7441 gcgtgatccc agtcgaggct gccggtgtgt tcggagccat tgtcttgcca acgggcgttc
     7501 accctcgtcg ctgtgctgtc atcgacacgc agaacgattg tgctgtcctc cgtggctctg
     7561 gcggtcactg tgtgccgcgt ctgggcctgc gcgacgtaca cgcggctgcg cgcatcgtgt
     7621 acggccgtgc ccagcgcccc cgcacaggcg gcggagacca gaacaacgaa gattcccagc
     7681 gcgataatca acagttcgag gcggtcggtg ccacgcacca gcggattgcg acggcaggcg
     7741 cgcagaagcc accatccgtg gacataatcg atcctttgca tgaccattcg cccgtcgaat
     7801 caggtgggtc acgaagtggg gacggctgtg tcgggtgcgg ggtggttgtt ggtgaccccg
     7861 agtcgatccg cgagcccttc caccgcggtg atgccgcggc gtccttggtg gatctggaag
     7921 gccagggcga tttcaccgac gccgtgcacc agcgctgcag cggccaccca cgcgaccaag
     7981 acgaccacgg agttgttcca cgacccggcg gcccagaacc ccaaacccag ctcgatgagc
     8041 ccggagatca gcagcaccca ccacccggac accatgttgg cggcgaaggc catgacaacg
     8101 tcgaaacagc cgcggaagat gaagtagaag ctgatgatgg cggccagcgt cacgaaggtg
     8161 gcaccgaagt tcgccaagga caccactccg gccacgacca gcagcgccgc cagcagaccg
     8221 tgcgcgatgc gccagccggt ggacgatgac agcgccccga tcatggtctc attgaccgcg
     8281 gcgaccaggc agtacacccc gaacagcacg gccaccgccg cgacggtggt gtagttgaag
     8341 cgcaggatca cgatggacag cacgatccag gcgacgccgg tgatcagcag caaccaccag
     8401 aaccgactct tggacacggc ttcgtcgaag aaacgaccaa ccgccccagc gggtgtcgtg
     8461 gtggcctgag gacgggtctc gctcattggt actcctgaac atttctattg atgcagcctc
     8521 gtcggtgata gtgagtcaag agtgctctcg gccgtaaacg attgcgatgt gctgcgacac
     8581 aacgtcgctg tcgcgatgaa gtgccggttg acaacgccca tacgggcaca gtttcggttg
     8641 tgacggtagc caaccgccgt tggcagatgg tggcccgccg aacaatgacc gctcgtatgc
     8701 gagcactcac gattggtggg agcagaaact gcaaccatga aggggcatcc atgtcaacgc
     8761 ttagcaaact ccatgaaagt gcggcaaaac accacgagca cgctgcacgg caccaccgcg
     8821 aagcggccaa gcttcacgag gtgaaagacg ttctggcggc ggtggatcag gcgcatatgg
     8881 cgaccgacca ccaggtgcac gccatccact acgcaacgca agctgcgaaa gagtacctgg
     8941 cggcgcaccg cggcagctag aagcctcgtc ggcgggaggc acaggcattc ggttcgtcca
     9001 gcccatcttc gttccgttgc gggcgtcggc cgtcaggtca acgcccgcaa ccgtgtaatg
     9061 ccatggccga agctcagctc ggcggcgact gcaagatcac gattgaccgc ggcccgaccg
     9121 taagctcttg cgcgacagtg cctttccgtg ccggatcgaa ggtgtcgcag acgacatccc
     9181 accggcgagc aggtagatta cctggcacgg caaaggccaa tggctcccac cacgcgttga
     9241 tcaacacgag aaagtcgttg tcgaccatcg gcgtgccgtc cacgccgacg tcgggatcgg
     9301 ttgagccgtc gataaataac gcgacgcaaa gggcgttcgg gtcagcccag ttctcggtcg
     9361 tcatctcggt acccgccggt gtgaaccacc ttaggtccgc ggcttctttg ccggtggagt
     9421 agcgacgcct gcggaaaacc gggtggcggc ggcgcagcgc gatgagctcc tgagtaaacc
     9481 ggagcaagtc ctcatcgacc gcggaccagt cgaaccacgt gatctcgttg tcctggcagt
     9541 acgcgttgtt gttgccgcgc tgggtgcgac cgagctcgtc acccccgagc aacaacggca
     9601 cgccggcgga aagaagcagc gtcacaagga aagcccgctt ctgcctcgcc cgcacggcat
     9661 tcacgccagt gtcgcccgtc ggcccctctg ctccgcagtt ccaggaccgg ttgtcgtcgg
     9721 tgccgtcgcg gttgtcttcg gtgttggcct cgttgtgctt acggtcgtac gaaacaaggt
     9781 catggagggt gaacccatcg tgcacggtga cgaagttcac cgatgcgctg ggtcggcgcg
     9841 cttcgtcggg ccggtcatag atatcggcag atccgcacag gcgcgtcgcg aagtccggca
     9901 gcagccgatc gtggctgcgc caccagtcgc gcacggtgtc gcggaagcgc ccgttccact
     9961 cgctccataa gggcggaaac cggccgatgt cgtagctgtc catgcggccg acatcccatg
    10021 gctccgcgat gagcttcacc tgcgacacaa ccggatcctg gccgacggtg tcgaagaacg
    10081 ccgaaaacgg gtcgaagtca ccatcttccc ggccgagggt cggcgccaga tcaaaccgga
    10141 aaccgtcgac gcccatctcg gacacccagt agcgcagcga atccatgatc atccgcagcg
    10201 ttgtcgcgtc tgcggtgttc agtgaattgc cgcacccggt cgtgtcgacg tagcgattga
    10261 gatcactcgg ctcgagtcgg taataggcag cgttatccag cccgcgatga cacagcgtcg
    10321 ggccgccggc acctccttca gcggtgtggt tgaagacgac gtccaacacc acttccagac
    10381 cggccgcgtg caacgcggcg accatgttgc ggaattcctc gacctggcca ccgggctgac
    10441 ccgcccgcac cgcggccgag tacgcggcgt gcggggcgaa gaagccgatc gtgttgtagc
    10501 cccagtagtt cgtcaatccc cgctcgacga ggaaggactc gggcacgttg tggtggatcg
    10561 gcagcagctc gaccgtggtg acgccgaggt cgacgaggtg tcgcaccgcc gcctcgtggg
    10621 ccaagcccgc gtaggtgccg cgcagttcct gtggcacgcc cggatgcttc gcggtaaatc
    10681 cgcgcacgtg cacctcgtag aggatcgtgt cggccagggc gtgcctcggc cctagcggta
    10741 gcggcgcgaa ggcgggggcc gtcaccaggc tgcgcggcat ctgacccgcg gaatccagcg
    10801 ggctgggtgc accgctgtta tccgttgcgt aatcgagcaa ttcgggcccg aaccggacgt
    10861 cgccgtggat cgctcgggca taggggtcga gcaggagttt ggcagggtta taacgctggc
    10921 cgcgcgggcg attgtacgag ccgctgaccc gataaccgta ggcctgaccc ggcgccacac
    10981 cgggcacgaa cccgtgccag acgtcgccgt cacgctccgg cagcggcaac tgggtctcgc
    11041 tgccgtcggc actgaaaagg cacagcctaa cctcatcacc gttggacgcg acggcgaagt
    11101 tcgtgcctcc gggcccaggc gtcgcaccga gcggggcggg acgacccgga aggacggtgg
    11161 cgctcacgcg gtctcctctc cctgaaccag gcgctgtatg acttccgacg ccttggggta
    11221 agcgtggtgg cgtcgcctga tcacatcggc gaccagcccg gtccaccccg tctggtggga
    11281 cgcgccgagg cccgcaccgg tgtcgccgtg gaagtactca tgaaagagca ggttgtcacg
    11341 ccatcgggga tcggtctgca tccgctgcac gccgccgaag caaggacgcc ttccgtcgtg
    11401 gccgaccagg a
//
LOCUS       DASRJA010000133         5854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_463908,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000133 DASRJA010000000
VERSION     DASRJA010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463908"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFC01_06060"
     CDS             complement(<1..544)
                     /locus_tag="VFC01_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein"
                     /protein_id="HZN79239.1"
                     /translation="MDRLDLRLVPAALTSWAVTAAGILWQVGGSVVAVAVAIVATAAV
                     GRWGSARREWDAGSGAIGAGIVAVAVVGAGFAVAIGLRVGELRHHPIVEHYGTVAPVV
                     VTATETPRSLGGGRLMFRGSLQQIFRSEISGRVVVFTRGAEFADVTAGRPASFRARIG
                     RPTRRDLTVAVLSATGEPKLG"
     gene            complement(562..1383)
                     /locus_tag="VFC01_06065"
     CDS             complement(562..1383)
                     /locus_tag="VFC01_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HZN79240.1"
                     /translation="MRTELPAERLQRRLGGDSESAGDGDGEPDTALSRWLPDSTKSDG
                     AAGWIAAVRADPGRAGVIALAVVGVIAVLVTVFTLIRDEPPPVTSAKLPPVEMVSSAS
                     PTPGPAPSNAEGPVVVSVVGLVHKPGLVTLAPGARIADALTAAGGPLDGADLIGLNMA
                     RRVTDGEQIIVGIVAAPGQAAAMGSSISSPPATAAPTDNPSATGNDSAPGGLVDLNSA
                     TVEELDKLPGVGPVTAAAIVAWRDANGRFTSVDQLGDVDGIGPARLEKLRDLVRV"
     gene            1497..2165
                     /locus_tag="VFC01_06070"
     CDS             1497..2165
                     /locus_tag="VFC01_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79241.1"
                     /translation="MDRRIPMIAGIAFAVLFTAALLLVPTLPGIDKPGYDIVSHINEH
                     SGAMRMQVLLVTFGSLALVVVLGYARDRLQGPSAYVFTIGSAVVLVQVFIAMWFTAGL
                     ALHPDQLGSATARTITDIVTMWGPILTVADIMVAAPILLAANNDRFPRWLGILAAVFA
                     VEQLIETITIIGPPASFISPGGAMNFYLGGPLFIIFFLGLGVALSLEQPETDQPATAD
                     EPAD"
     gene            complement(2338..2754)
                     /locus_tag="VFC01_06075"
     CDS             complement(2338..2754)
                     /locus_tag="VFC01_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HZN79242.1"
                     /translation="MPVFTIDAPLGAAPSAKQKMMKDIHDALDEAYHFPDTRGWLREY
                     PGENVSQDGRVLAEPVRPVCSLEAPELASLDAKRKLVQKIEGAIGEAYVGVANVDEVL
                     VLINHYPLRNVGWRHSLQSDKPEIVAAVDQLNASIG"
     gene            2869..3525
                     /locus_tag="VFC01_06080"
     CDS             2869..3525
                     /locus_tag="VFC01_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79243.1"
                     /translation="MVGRRKYDDGCAIAQALDVIGERWALLVVRELVLGPKRFTDLLA
                     GLHGASSDVLTQRLRDLSAAGVVRRRRLGPPVSSWVYELSEWGAELEDIILGLAHWVH
                     KSPHMRYDLPLGADSLMLALKTRFHPPAADGLDATVGIHLGDAHFRVRIADGQIAIDR
                     GEADSTDLVLETDQNTLLSLLGPDGSVDDALTSGQLRLIGDEAIAETFRGLFPVPESG
                     "
     gene            complement(3522..4628)
                     /locus_tag="VFC01_06085"
     CDS             complement(3522..4628)
                     /locus_tag="VFC01_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79244.1"
                     /translation="MKIELSAGNARVYEVRAHIRSSVVVGVAMIGIGALASAPMAPPP
                     EPHAPPTVSREVRLTAAPALGSIPLAFIRNQFQYCSLICPHAVEGAGTVPLAAAQAPA
                     TFLGTLTSTGSPLKAFGAAAASVTGPANAAVTPLINNDVFLVVPKAFHALDVAVVEAV
                     NVGAAALTPEEFLQAIQTGRTNILNALNQPVGTPTTPTGATNIVQVVAVEAINVTTAV
                     AFQAGELLLSGAVQIADASAQELAKTGNPASALAAGAAEARQVAATASAPVVSAVNTA
                     VTNIRNSLHDPFPAVAETTVRTDEPKRAEPPTTKTVSESRSGKKDSDRGMVTSQTSAA
                     LADVEHPAKHERKVHDPVSSKDGRPANRTHSNAG"
     gene            4656..5285
                     /gene="nthB"
                     /locus_tag="VFC01_06090"
     CDS             4656..5285
                     /gene="nthB"
                     /locus_tag="VFC01_06090"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HZN79245.1"
                     /translation="MDGVHDLGGLDGFGPVEHATTEPYFAEDWERRVFRFMIGSLGTL
                     SINGGMFRHSIERMDPAHYLSSPYFEHWLTGVTTLAVEAGAASVDELERRAGGRFPLS
                     RPDRGLLPDDLTPRKEPRYAVGDEVRVREWHPPGHTRAPRYVQGKRGTIVRFDGAHNV
                     SDIEAHGGGFVTDPVYSVRFASRELWGEAGTDGDVIHVDLFERYLEDSQ"
     gene            5282..>5854
                     /gene="nthA"
                     /locus_tag="VFC01_06095"
     CDS             5282..>5854
                     /gene="nthA"
                     /locus_tag="VFC01_06095"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HZN79246.1"
                     /translation="MTADHHHPAELAPVEARIAAIESVLVEKGLLDTEAVDAIVRNFE
                     DNLGPMNGAKVVARAWTDQAYKERLLADGTAAIRELGFEGPEGDYMVVVENTPEVHNL
                     VVCTLCSCYPWPTLGLPPKWYKAPAYRSRAVREPRKLLAEMGTVIPDGVEIRVWDSSA
                     EARYLVLPMRPDGTDEMTEEQLAGVVTRDSM"
BASE COUNT          988 a         1981 c         1875 g         1010 t
ORIGIN      
        1 cgcccagctt cggctcaccc gttgccgaca gcaccgccac cgtcagatct cgccgtgtgg
       61 gtctgccgat gcgggcccgg aagctcgccg gccgaccagc cgtcacgtcg gcgaactctg
      121 cgccgcgcgt gaaaacgact acccgcccag atatttcact ccgaaagatc tgctgcagcg
      181 acccgcggaa catcagtcgg ccgccgccca gtgagcgagg cgtctcggtt gctgtgacca
      241 cgacgggggc gaccgttccg tagtgctcga cgatcggatg gtggcgcagt tcgccaacac
      301 gcaacccaat cgccacggcg aaacccgcac cgaccaccgc gacagcgacg atgccggcac
      361 cgatcgcccc ggaccccgca tcccactcac gccgcgcact accccacctc cccaccgctg
      421 ccgtcgcgac gatcgccacg gcaacggcca ccaccgaacc gcctacctgc cagaggattc
      481 cggctgccgt caccgcccaa ctggtgagcg ccgccgggac cagtcgcagg tctaaccggt
      541 ccatgccggt agcgaggacc gtcacacgcg gacgaggtca cgtagcttct ccagccgcgc
      601 gggcccgatg ccgtccacgt caccgagctg gtcgacgctg gtgaaccgcc cgttggcgtc
      661 acgccaggcg acgatcgcgg ccgcggtgac cggaccgaca cccggcagtt tgtccagctc
      721 ctccaccgtg gctgaattca agtccaccaa accgcctggg gccgagtcgt ttccagtggc
      781 cgacgggtta tccgtcggtg cggctgtggc cggcggcgaa ctgatcgaac tacccatcgc
      841 tgcggcctgg ccgggtgccg cgacgatccc aacgatgatc tgttcaccgt cggtgactcg
      901 ccgtgccatg ttcagcccga tcaggtcggc accatccaag gggccgccgg cggccgtgag
      961 cgcatcggcg atccgtgcac caggcgccaa cgtgacaagt ccgggcttat gcacgaggcc
     1021 caccacgctg acgacgactg gaccctcggc gttggacggc gcgggccccg gcgtcgggct
     1081 ggccgacgaa accatctcca ccggcggcag tttcgccgac gtcaccggcg gcggctcgtc
     1141 gcgtatcagc gtgaagaccg tcaccaatac ggcgatcaca ccgacgacgg ccaacgcgat
     1201 gacgccggcg cggccggggt ctgcccggac agcggcgatc cagccagccg ccccatcgga
     1261 tttggtcgag tcgggcagcc atcgcgacag cgcggtgtcc ggttcaccgt cgccgtcgcc
     1321 agccgactca ctgtcgccac cgagacgacg ctgcaggcgt tcggcgggca attcggttcg
     1381 catgccgtca aagtaggcgc gcgctcggac ctctcggccg tgtgaggcgt cccggcaacg
     1441 cgccctgtgg atgaaatccc gtgtgtggat aaccgcggcc tctacagtga tttgatatgg
     1501 atcgtcgcat tccgatgatc gctggtatcg cgtttgcggt gctgttcacc gctgcgctgc
     1561 ttttggtccc gacgcttccc ggcatcgaca agccgggcta cgacatcgtc tcgcacatca
     1621 acgaacattc cggcgcgatg cgaatgcagg tgctgttggt gacgttcggg tcactggcgt
     1681 tggttgtggt gctcggctac gcgcgcgatc ggctgcaagg cccgtcggcg tacgtgttca
     1741 cgatcggctc tgcggttgtg ctggtccagg tgttcatcgc gatgtggttc acggccggac
     1801 tcgcgctgca tcccgaccag ctcggatcgg ccactgcgcg caccatcacc gacatcgtca
     1861 cgatgtgggg gccgatcctg acagtggccg acatcatggt ggcggcacca attctgttgg
     1921 cggccaataa tgatcgattc ccgcggtggc tcgggatact ggccgcggtg ttcgccgtcg
     1981 agcagctgat cgagacgatc acgatcatcg gcccgcccgc cagcttcata tccccaggcg
     2041 gcgcgatgaa tttctacctc ggcgggccgc tgttcatcat cttcttcctc ggcctcggtg
     2101 tcgcattgtc tttggagcag ccagaaacgg atcagcccgc gaccgcagac gaaccagccg
     2161 actgatccag cgacgccggc acctcgcaac cataggccga caccactgcg accgcacata
     2221 ccgccgtcac gacggtctcg gctccgtagc gtaggaaaac gcgataccgg gaccgagcgg
     2281 caagacccgg ccccgtatcg gcgttagtgc cggaaaatta ctacgccgaa gcaggtttca
     2341 gccgatgctg gcgttcagct gatctactgc cgcgacgatc tccggcttgt cggattgcaa
     2401 gctgtgccgc cagccgacgt tgcgcaatgg gtaatggttg atgaggacga gcacctcgtc
     2461 gacgttggca accccgacgt atgcctcccc gatcgccccc tcaatctttt gcacgagttt
     2521 gcgtttggcg tcgaggctgg ccagctcggg cgcttccaac gagcacaccg gtcgcaccgg
     2581 ctccgccagc acacggccgt cctgcgacac gttctcgccc gggtactccc gcagccagcc
     2641 gcgagtgtcg gggaagtgat aggcctcgtc gagcgcatcg tgaatgtcct tcatcatctt
     2701 ttgcttcgcc gacggagccg ccccgagagg cgcatcaatg gtgaatacgg gcatttcttc
     2761 atctccttgg tcagttgtcg agagccgaca cttggacaat acatcttttt agttggaaaa
     2821 accaagtgat tagttcgagt acccgatttc tgctggtaag atgggcgcgt ggtaggtagg
     2881 cggaagtacg acgacggatg tgcgatcgcg caggctctag acgtcatcgg cgagcggtgg
     2941 gcactgctcg tggtacggga gctcgtgctt ggccccaagc gattcaccga ccttctggcg
     3001 ggtctgcacg gagcgagttc ggacgtgctc acacagcggc tgcgcgatct cagcgcggca
     3061 ggggtcgtgc gccggcgtcg gctcggaccg cctgttagct cctgggtgta tgagctgtcg
     3121 gagtggggcg ccgagcttga ggacatcatc ctcgggctgg cgcactgggt gcacaagtcc
     3181 ccacacatgc gctacgacct gccactcggg gcggactcgt tgatgctggc actgaagacg
     3241 cgtttccacc cgcccgccgc cgacggcctg gacgcgacgg tcggcattca cttgggggac
     3301 gcgcattttc gcgtccgcat cgccgacggc caaatcgcca tcgatcgtgg cgaagccgat
     3361 agcacggacc tcgttctcga gaccgaccag aacacactgc tatcgctgct gggtcccgat
     3421 ggctccgttg acgacgcgct aacgagtgga cagctacgcc tcatcggcga tgaggcgatc
     3481 gccgaaacgt tcagagggtt gttcccggta cccgagtcgg gctaacccgc attcgagtgg
     3541 gtgcggttgg cggggcggcc atccttggag ctcaccgggt cgtgaacctt tcgctcgtgc
     3601 ttggccggat gctcgacatc ggccaacgcg gcactagtct ggcttgtgac catccctcga
     3661 tccgaatcct ttttcccgct tcggctttcg gatacggtct ttgtggtggg cggctccgcc
     3721 ctcttcggtt cgtcggtcct gaccgtcgtc tccgcaacgg ccgggaacgg atcgtgcagt
     3781 gagtttcgga tgttggtcac cgcggtgttc accgccgaga cgacgggcgc gctcgccgtg
     3841 gcggccacct ggcgcgcctc cgcagccccg gccgccaggg cggacgccgg attaccagtc
     3901 ttcgcaagct cctgcgccga agcatcggcg atctgcaccg cgcccgacag caacaactca
     3961 ccggcctgaa acgcgactgc cgtcgtgaca ttgatggcct ccaccgccac cacctgcacg
     4021 atgttcgtgg cacccgtggg tgtcgtcggc gtgccaaccg gctgattgag ggcgttgaga
     4081 atgttggtgc gccctgtctg gatcgcctgt aagaactctt ccggcgtcaa tgctgccgca
     4141 cccacgttga cggcctcgac gacggcgaca tctagcgcgt gaaaagcctt cggcaccacc
     4201 agaaacacgt cgttgttgat cagcggggtg accgcggcgt tcgcgggtcc ggtcaccgac
     4261 gcggcggccg cgccgaacgc cttcagcggc gaacccgtcg acgtgagcgt gccgaggaac
     4321 gtcgccggag cctgcgccgc ggcgagcggc acggtgcccg caccctcgac cgcgtgcggg
     4381 cagatcaggc tgcagtactg aaactgattg cggatgaacg ccagtgggat cgaccccagc
     4441 gcaggcgcgg ccgtcaaccg gacctcgcgc gacacggtcg gcggtgcgtg aggctccggt
     4501 ggtggtgcca tcggcgcaga agccagcgcc ccgatcccga tcatggcaac gccgacgacg
     4561 acagacgaac ggatatgcgc acgcacctcg taaacacggg cgttgccggc ggaaagttcg
     4621 atcttcaacg aatcgatcgc cgtaggattc tcgacgtgga cggcgttcac gacctcggcg
     4681 ggctcgacgg gttcggcccg gtcgagcacg cgacaaccga gccgtacttc gccgaggatt
     4741 gggagcggcg tgtattccgg ttcatgatcg ggtccctcgg cacgctctcg atcaacggtg
     4801 gcatgttccg ccactcgatc gagcgcatgg accctgcgca ttacctctcg tcaccgtact
     4861 tcgaacactg gttgaccggt gtcaccacgc tggccgtcga ggcgggtgcg gcctctgttg
     4921 acgagctcga gcgccgcgcc ggcggccgat ttccactgtc ccggcccgac cgcggcctac
     4981 ttcccgatga cctcacgccg cgcaaggagc cgcgctacgc cgtcggcgac gaggtccgcg
     5041 tccgcgaatg gcacccgccc ggacacaccc gcgcaccccg ctacgtgcag ggcaagcggg
     5101 gaacgatcgt gcggttcgac ggcgcgcaca acgtctcgga catcgaggcg cacggtggcg
     5161 gattcgtgac cgatccggtg tattcggtgc ggttcgcctc gcgtgagctc tggggcgagg
     5221 cgggcaccga cggcgatgtc atccacgtcg acctgttcga gcgctacctc gaggattcac
     5281 aatgacggcc gaccaccatc acccagcgga actggcgccg gtcgaggcca ggatcgccgc
     5341 aatcgaatcg gtgctcgtcg agaagggcct gctcgacacc gaagcggtcg acgccatcgt
     5401 ccgaaacttc gaagacaacc tcgggccgat gaacggcgcg aaagtggttg cccgggcctg
     5461 gaccgatcag gcttacaagg agcgcctcct cgccgacggt acggcggcga tcagggaact
     5521 cggattcgag ggacccgaag gcgactacat ggtcgtcgtc gagaacaccc cagaggtgca
     5581 caacctggtg gtgtgcacgt tgtgctcctg ctacccgtgg ccaacgctcg gcctcccgcc
     5641 gaagtggtac aaggcaccgg cctaccggtc acgcgccgtg cgtgaaccgc gcaagctgct
     5701 cgccgagatg ggcacggtca tccccgacgg cgtcgagatc cgggtgtggg attccagcgc
     5761 cgaggcgcgc tatctggtgc tcccgatgcg cccggacggg acggacgaga tgacggagga
     5821 gcagctcgcc ggcgtcgtca cccgtgactc gatg
//
LOCUS       DASRJA010000134         9824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3559483,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000134 DASRJA010000000
VERSION     DASRJA010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9824
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3559483"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..1231)
                     /gene="meaB"
                     /locus_tag="VFC01_06100"
     CDS             complement(251..1231)
                     /gene="meaB"
                     /locus_tag="VFC01_06100"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HZN79247.1"
                     /translation="MTTPVEELAAAVRSGDRAALPRAITLVESTRPDHREQAQQLLLE
                     LMPEASNAMHVGITGVPGVGKSTTIEELGMHLIEQGHRVAVLAVDPSSTRTGGSILGD
                     KTRMARLAVHPDAYIRPSPTSGTLGGVTKATRETIVLLEAAGFDVILVETVGVGQSEV
                     AVANMVDTFVFLTLARTGDQLQGIKKGVLELADIVVVNKADGEHAQEAKAAARELTGA
                     IRLIYPRETLWRPPVLTMSALHGSGLTELWEMVLKHRQVLTDAGEFEARRRAQQVDWT
                     WSMVRDTVLDRVLSNPEVRRIRAEVERQVRDGELTPALAAQQLLDAADMG"
     gene            complement(1232..3454)
                     /gene="scpA"
                     /locus_tag="VFC01_06105"
     CDS             complement(1232..3454)
                     /gene="scpA"
                     /locus_tag="VFC01_06105"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HZN79248.1"
                     /translation="MTATTGIGSFADVPLHGERDGEPATEATVAKHVEAAAAAHGYTA
                     DQLYWSTPEGIDVKPVYIAADRDNAVAHGYPLDSFPGEPPYIRGPYPTMYVNQPWTIR
                     QYAGFSTAAESNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVQGDVGMAGVAIDS
                     ILDMRQLFDGIDLSTVSVSMTMNGAVLPILALYVVAAEEQGVPPEKLAGTIQNDILKE
                     FMVRNTYIYPPKASMRIISDIFGYSSARMPKFNSISISGYHIQEAGATADLELAYTLA
                     DGVEYIKAGLDAGLDIDKFAPRLSFFWGIGMNFFMEVAKLRAGRLLWSELVAEFNPKD
                     AKSLSLRTHSQTSGWSLTAQDPFNNVARTCIEAMAATQGHTQSLHTNALDEALALPTD
                     FSARIARNTQLLLQQESGTTRPIDPWGGSYYVEWLTHQLAERTRAHIREVDEHGGMAQ
                     AISDGIPKLRIEEAAARTQARIDSGQQPVIGVNKYQVDEDHEIEVLKVENSRVRAEQI
                     AKLQQLRAERDQAATDAALAELSKAAGSEGNLLELAINAARAKATVGEISDALEKVWG
                     RHQAEIRTIAGVYRDELGKVSNISQANELVEKFAHADGRRPRILVAKMGQDGHDRGQK
                     VIATAFADIGFDVDVGSLFSTPEEVARQAADNDVHVVGVSSLAAGHLTLVPALRNALA
                     EVGRPDIMIVVGGVIPPGDFDELYAAGATAIFPPGTVIADAAIGLLQKLAERLGYELN
                     "
     gene            complement(3456..5246)
                     /gene="mutA"
                     /locus_tag="VFC01_06110"
     CDS             complement(3456..5246)
                     /gene="mutA"
                     /locus_tag="VFC01_06110"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase small subunit"
                     /protein_id="HZN79249.1"
                     /translation="MQKTGDTDSDIARWRSAVAGVLAKSTRRDAADLPAEPERLLDSQ
                     TYEGFPIRPLYTSLDALPEPALPGQWPYVRGGDALRDVKSGWRVAEAFPANGSSAAEA
                     NGAVLLALTEGVSALVLRVGGVAAELDRLLDGVFLDLVPVVLDAGADYIAASEAVLAL
                     LTDLDDDQRSRLSVDLGADPLTAPLSGRPAPNVADFVAVATKVTGYGGAVRAITVDGP
                     AFHDLGASASWELAGSIAAAVSYLRVLGDGGIVPGDALRQISFRFAADDDQFMTIAKL
                     RAARQLWARVAEVVGEPDAGAARLHAVTSVPMMAQRDPWVNMLRTTVAAFSAGAGGAD
                     TVLVHPFDVAIHGGFPGTAASFARRIARNTQLLLLEESHIGRVLDPAGGSWFVEDLTK
                     GLAEQAWKHFQDIESRGGFEAARDYVAGQIKEVADGRSDDIAHRRKALTGVNEYPNLT
                     ESPLPQGDSLETVVRYAAGFEALRNRSDAFLEKTGSRPTVLLLSLGPLAEHNVRTTFA
                     TNLLASGGIEAVTEDANAKIAVICGTDARYGTEASTAVDAARTAGVSYVLLAGPEKAV
                     AEADSKPDGYLTAKIDAVTTLSDLLTRLGA"
     gene            5352..6098
                     /locus_tag="VFC01_06115"
     CDS             5352..6098
                     /locus_tag="VFC01_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HZN79250.1"
                     /translation="MKAVVSTLRALGAAVVATASSVPPRRFVAILTAIVILVAVAVLV
                     PLPTAVQLRDWARSVGPWFPMAFLAAHIVVTVFPFPRTAFTLAAGLLFGPYLGVPLAV
                     LASAASAVIALLLVRAAGWQLSRLVRHSRVDSVDARLRERGWPAVISMRMIPAVPFSV
                     VNYAAGASAVRVLPYTLATLVGLLPGTAAVVILGDALTGNVSPLLLVVSLCTASLGFA
                     GLIYEIRIHRRHHRERLADSAPDTEPAITG"
     gene            complement(6099..7352)
                     /locus_tag="VFC01_06120"
     CDS             complement(6099..7352)
                     /locus_tag="VFC01_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZN79251.1"
                     /translation="MEGAVAGLILVGVLVVFAAIIVAKSIALIPQAEAAVIERLGRYS
                     KTVSGQLTLLLPFVDKIRARVDLRERVVSFPPQPVITEDNLTVNIDTVVYFQVTNPQA
                     AVYQISNYIVGVEQLTTTTLRNVVGGMTLEQTLTSRDQINGQLRGVLDEATGRWGLRV
                     ARVELRSIDPPPSIQDSMEKQMRADREKRAMILTAEGSREASIKQAEGQKQAQILAAE
                     GAKQAAVLAAEADRQSRMLRAQGERAAQYLVAQGQAKAIEKTFAAIKAGRPTPEMLAY
                     QYLQTLPQMAKGEANKVWLVPSDFGAALQGFTKMLGAPGEDGVFRYQPSPVEENLPKP
                     EDDSDEVAEWFTTQTDPEIARAVAKAEAEARTPVQGAIPGAPTPADPPLQYPPLSQQR
                     QDGPPLPPGATAPITRDGGGGAHSL"
     gene            complement(7385..7816)
                     /locus_tag="VFC01_06125"
     CDS             complement(7385..7816)
                     /locus_tag="VFC01_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZN79252.1"
                     /translation="MPVALIWLIAALALAGAEALTGDLFLLMLGGGALAAAGSSLLVD
                     DLWVHGAVFLVVSVLLLVLVRPALKRHFMSGKGLPEPAKALEGKSALVLDRVARHEGQ
                     VKLDGEIWTARPLNDDDIYEPGDHVTVVHIDGATAVVQKVV"
     gene            7876..8718
                     /locus_tag="VFC01_06130"
     CDS             7876..8718
                     /locus_tag="VFC01_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79253.1"
                     /translation="MWCPSVSLSVWANAWLAGAAAPDDVLDALSQWAPRHSVTAYDSV
                     AAGRTGLPWPDLNDVGAVSLLQTARTAAGTGLSAPAIGVALPVPGDVRGLPAGTQFQR
                     DAVTAGEAVIVGRDASTAVGLVPEFEYDELEDESEFEPEPTSLSWTVYSVPAGAPAEY
                     LDLGEAEYALRAAVRSAADALGAIRAAGTDVADPRGLVEQVLEGTRGHRVPDHAPSRA
                     VRVLENAAHIDAIITVSSGLIPIGLQTSSEVQIASDALRPLSGVVRSARMAAISEILY
                     SAWR"
     gene            8770..9048
                     /locus_tag="VFC01_06135"
     CDS             8770..9048
                     /locus_tag="VFC01_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79254.1"
                     /translation="MNTPTAPRIWTIVTAGAIALGVALTVNAPAAQAERISESTIKSE
                     CRAAGGTYSTTVIKGIGERHSSCTYKDEDGNKWTDVYTNGEYQGTDPA"
     gene            complement(9045..>9824)
                     /locus_tag="VFC01_06140"
     CDS             complement(9045..>9824)
                     /locus_tag="VFC01_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZN79255.1"
                     /translation="PVYFGNRNWEPYVEDTVATMRDNGIRRAAVFTTSAWGGYSSCTQ
                     YVEDIARARAAAGEGAPELVKLRQYFDHPLFVEMFTEAIGESAQTVPDDARLVFTAHS
                     IPVVAQNRCGADLYSRQVSHAARLVATAAGYDDYDQVWQSRSGPPQVPWLEPDIADHL
                     ATLAEAGTKAVIVCPIGFVADHIEVVWDLDHELQQQARDLDVAFARAATPNADPRFAR
                     LAADLIDELRHGREPVRVPGPDAPPLQGLSVNGVVCTPNCG"
BASE COUNT         1558 a         3338 c         3239 g         1689 t
ORIGIN      
        1 tctgcattgc accaacatag ggtcaaacac tgacaacccc gaccgatcgc cgcgccggac
       61 acagcgcggg ccgtgctcca cgctcatccg ttggtcatga ccaaatgtcg gccagcgcga
      121 gcacttgccg atcatcaata catcgacgga cgaacccgac aacctcaccc gccaggctcc
      181 gcccggctca tcgacgcgac ggactagctc cgggccaaag ccccaacctt gcgtccaaga
      241 ataatcgcgg tcatcccatg tcagcggcat cgagcagctg ctgggcagca agggcggggg
      301 tcagctcgcc gtcccggacc tgacgctcca cctcggcccg aatcctgcgc acctcgggat
      361 tcgacagcac ccggtccagc acggtgtcac gcaccatcga ccacgtccag tccacctgct
      421 gcgcacgcct gcgcgcctcg aactcccccg cgtcggtgag cacctggcga tgcttgagca
      481 ccatttccca gagctcggtg aggccgcttc catgtagcgc gctcatcgtg agaaccggtg
      541 ggcgccaaag cgtttcgcgt gggtagatga ggcggatcgc gccggtcagc tcccgggccg
      601 cggccttggc ctcctgcgcg tgctccccgt cggccttgtt caccacgacg atatccgcga
      661 gctcgagcac gcccttcttg atgccctgca gctgatcgcc ggtgcgcgcc agggtcagga
      721 acacgaacgt gtcgaccatg ttcgcaacgg ccacttcgga ttgccccacg ccgaccgtct
      781 ccaccaggat gacgtcgaac ccggccgcct cgagcagcac gatcgtctcg cgcgtggcct
      841 tggttacccc gccgagcgtg cccgacgttg gcgacggccg gatgtaggca tcgggatgca
      901 cggcgagccg agccatgcgg gtcttgtcgc cgaggatcga tccgccggtg cgggtggacg
      961 aggggtcgac ggccagcacg gcgacccgat gaccttgctc gatcaggtgc atcccgagct
     1021 cctcgattgt ggtcgacttg ccgacgccgg gcactccggt gatgccgacg tgcatggcgt
     1081 tgctggcttc cggcatcagt tccagcagca gctgctgcgc ctgctcacga tggtcggggc
     1141 gggtcgattc aaccagcgtg atggccctcg gcagcgccgc ccggtcgccg ctgcggacgg
     1201 ccgccgcgag ttcttcgacc ggagtggtca tctagttcaa ttcgtagccc agccgctcgg
     1261 ccagcttttg cagcagcccg atagcggcgt cggcgatcac cgtgcccggc gggaaaatgg
     1321 ccgtggcgcc cgcggcgtac aactcgtcga agtcgccggg cgggatgacg ccaccgacga
     1381 cgatcatgat gtcgggccgg cccacctcgg cgagcgcatt gcgcagcgcg ggcaccagcg
     1441 tgaggtgtcc ggccgccagc gacgacaccc cgacgacgtg cacgtcgttg tcggcggcct
     1501 ggcgagccac ctcctccggc gtggagaaca gcgagccgac gtcgacgtca aagccgatgt
     1561 ccgcgaacgc cgttgcgatc accttctgcc cgcggtcgtg cccatcctgg cccatcttgg
     1621 cgacgaggat gcggggccgg cgaccgtcgg cgtgggcaaa cttctcaacg agctcgttgg
     1681 cttgcgaaat attgctgacc tttccgagtt cgtcgcggta gaccccggcg atggtacgga
     1741 tctcggcctg atggcgtccc caaaccttct cgagcgcatc ggagatctcg ccgacggtgg
     1801 ccttggcccg cgccgcgttg atcgcgagct cgagcaggtt gccttcggac ccagcagctt
     1861 tggacagctc ggccagcgcg gcgtcggtcg cggcctggtc ccgctcggcc cgcagctgct
     1921 gcagcttggc gatctgctcg gcacgcaccc ggctgttctc gaccttgagc acctcgatct
     1981 cgtggtcctc gtcgacctgg tacttgttga cgccgatcac cggctgctgt ccggagtcga
     2041 tgcgggcctg agtacgggcg gcggcctcct cgatacgcag cttcgggatg ccgtcgctga
     2101 tcgcctgcgc catgccgccg tgctcgtcca cttcacggat gtgcgcgcgg gtgcgctctg
     2161 caagttgatg cgtcaaccac tccacgtaat acgagccacc ccacgggtcg atcggacggg
     2221 tggtgccgga ttcctgctgc aggagtagct gcgtgttgcg cgcgatccgc gcggagaagt
     2281 cggtcggcaa cgccagcgcc tcgtcgagcg cgttggtgtg cagcgactgg gtgtggccct
     2341 gggtcgcggc catcgcctct atgcaggtgc gcgcgacgtt gttgaacggg tcctgcgcgg
     2401 tcagtgacca gcctgaggtc tgcgaatgtg tgcgcaggga aagtgatttc gcgtccttcg
     2461 ggttgaactc ggcaacgagc tcactccaca acagccggcc ggcacgcagc ttggccacct
     2521 ccatgaagaa gttcatcccg ataccccaga aaaaggacag ccgcggcgcg aacttgtcga
     2581 tgtccaagcc ggcgtcgagg ccggccttga tgtactcgac accatcagcc aacgtgtatg
     2641 ccagctccaa atccgctgtg gcaccggcct cctggatgtg gtagccggag attgagatcg
     2701 agttgaactt cggcatcctg gcgctggagt agccgaagat gtcggagatg atccgcatcg
     2761 acgccttggg cggatagatg taggtgttgc ggaccatgaa ctccttgagg atgtcgttct
     2821 gaatggtccc ggccagcttc tccggcggca caccctgctc ctcggcggcc accacgtaca
     2881 gcgcgagaat cggcaaaacc gcgccgttca tcgtcatcga caccgatacg gtcgacaggt
     2941 cgatgccgtc gaacagctgg cgcatgtcga ggatggaatc gattgccacg cccgccattc
     3001 cgacgtcgcc ctgcacccgt ggatggtcgg agtcatagcc gcggtgggtg gccaggtcga
     3061 atgccaccga aagccctttc tggcccgcgg cgaggttgcg gcggtagaac gcgttggact
     3121 ccgcggccgt ggaaaagccg gcgtactgcc ggatggtcca cggttggttg acgtacatag
     3181 tcgggtacgg accgcggatg tacggcggtt cgcccgggaa gctgtcgagc ggatagccgt
     3241 gggccaccgc attatcccgg tcggcggcga tgtaaacggg cttgacgtcg atgccctcgg
     3301 gagtcgacca gtacagttgg tccgcggtgt agccgtgcgc ggcggcggcc gcttcaacgt
     3361 gcttggcgac ggtggcttcg gtggccggtt ctccgtcgcg ttcgccgtgc agtggcacgt
     3421 cggcgaaact tccgatgcct gttgttgcag tcacgtcagg cccccaatct ggtgagcagg
     3481 tccgacaacg tcgtcaccgc gtcgatcttc gcggtcaggt agccatcggg ttttgaatcc
     3541 gcttcggcca cggccttttc ggggccggcc aggagcacgt atgacacgcc cgcggtgcgc
     3601 gccgcatcga cggcggtcga ggcctcggtg ccgtaacggg cgtcggtacc gcagatcacc
     3661 gcgatcttgg cgttggcatc ctccgtgacc gcctcgatgc cgccggatgc gagcaggttg
     3721 gtcgcgaacg tggtgcggac gttgtgctcc gccagtgggc cgagcgacaa gagcaacacc
     3781 gtcggccgcg atcccgtctt ctccagaaac gcatccgacc ggttccgcag cgcctcgaac
     3841 cctgctgcgt agcgcaccac agtttcaagg gaatcaccct gcggcagggg cgattcagtg
     3901 agattcgggt actcgttgac gccggtgagc gccttgcgcc ggtgcgcgat gtcatcggag
     3961 cgaccgtcgg ccacctcttt gatctggccg gcgacgtagt cgcgggcggc ctcgaaacca
     4021 ccgcgggatt cgatgtcctg gaaatgcttc cacgcctgct cggccaagcc cttggtgagg
     4081 tcctcgacga accacgaacc gcccgcgggg tcgagtacgc ggccgatgtg cgactcctcc
     4141 agcaacagca gttgggtgtt gcgggcgatg cgccgcgcga aactcgctgc ggtaccgggg
     4201 aagccgccat ggatggccac atcgaacggg tgcaccagca cggtgtcggc accgcccgca
     4261 cctgccgaga acgccgcgac cgtcgtgcgc aacatgttca cccacggatc gcgctgcgcc
     4321 atcattggta ccgacgtgac cgcatgcagc ctggccgctc ctgcgtccgg ctcccccact
     4381 acctcggcca cccgcgccca cagctggcgc gctgcgcgca gcttcgcgat tgtcataaat
     4441 tggtcgtcat cggcggcgaa gcggaaactg atctgccgca acgcatcccc cggcacgatg
     4501 ccaccgtccc cgagtacccg cagataactg acggctgcgg cgatgctgcc cgccaattcc
     4561 cacgacgcgc tggcgccgag atcgtggaat gcaggcccgt cgacggtgat cgcccgcacg
     4621 gcaccgccgt atccggtcac cttggtggcg accgcgacga aatcggccac gttcggcgcg
     4681 ggtcgcccgc tcagtggcgc ggtcaacgga tcggcgccga gatccaccga cagccgtgaa
     4741 cgctggtcgt catcgagatc ggtcagcagc gccagcaccg cctcagatgc ggcaatgtaa
     4801 tcggcacccg cgtcgagcac gacgggaacc aggtcgagga agacaccgtc gagcagtcgg
     4861 tccaactcgg ccgccacgcc cccgacgcgc agcaccaatg cgctgacgcc ctcggtcaac
     4921 gcgaggagta ccgcgccgtt cgcctcggcc gcagaggaac cgttggcggg aaacgcctcg
     4981 gccacccgcc aaccggactt gacgtcacgc agcgcatcgc cgccgcgcac gtacggccac
     5041 tgcccgggca gcgccggctc gggcagcgcg tccagggacg tgtacagggg gcggatcgga
     5101 aaaccttcgt aggtctgcga atccagcagc cgctccggct cggcgggcaa atccgccgcg
     5161 tcgcgccggg tgctcttggc caggacgccg gctaccgccg agcgccacct cgcgatatcg
     5221 gaatccgtat caccggtttt ctgcacgcca tcaggctaaa tgatggccat cacagcagcg
     5281 tccgccggtc ggccgactca caacgacgct gagagcgtgg tgccgggtaa cggcccgtag
     5341 tcttggtaaa cgtgaaagcc gtcgtcagca ccctgcgcgc gctgggtgcg gcggtggttg
     5401 cgacggcgtc cagcgtgccg ccgcgcaggt tcgtcgccat cctgacagca attgtgatcc
     5461 tcgtcgcagt tgcggtgctg gttccgttgc ccaccgcggt ccagctgcgc gactgggcac
     5521 gctcggtcgg tccgtggttc ccgatggcgt tcttggccgc gcacatcgtg gtgacggtct
     5581 ttccgttccc ccgcaccgcg ttcacactgg ccgcgggcct gctgttcggc ccctatctcg
     5641 gtgtcccgct ggccgtgctg gccagcgcgg ccagcgccgt catcgcgctg ctgctggtgc
     5701 gcgctgccgg ctggcagctg agccggctgg tccggcactc ccgcgtggac tcagtcgatg
     5761 cgcggctgcg cgagcgcggc tggccggcgg tgatttcgat gcggatgatc ccggcggtgc
     5821 cattctcggt cgtcaactac gccgcagggg cgtccgcggt gcgtgtgctg ccctacacgc
     5881 tggccactct ggtcggtcta ctgcctggca cggccgccgt ggtgatcctc ggtgacgcgc
     5941 tgacaggcaa cgtcagccca ctgttgttgg tggtgtcgtt gtgcacggcg agcctcggct
     6001 tcgcggggtt gatttacgaa atccgcatcc acaggcgcca tcaccgcgag cgcctcgccg
     6061 actcggctcc cgataccgag ccggcgatca ccggctgact acagcgagtg cgctccgccg
     6121 ccaccgtcgc gggtgatcgg agcggtggcc ccaggcggca gcggcggacc gtcctgccgc
     6181 tgctgtgaca gcggcggata ctgcagcgga ggatcggcag gtgtgggcgc cccagggatg
     6241 gcgccctgca ctggggtgcg ggcctcggcc tcggccttcg ccacagccct ggcgatctcc
     6301 gggtcggtct gcgtggtgaa ccactcggca acctcgtcgg aatcgtcctc gggcttgggc
     6361 agattctcct caaccggcga tggctggtag cggaagacac cgtcctcacc cggcgcgccc
     6421 agcatcttgg tgaagccctg cagcgccgcg ccgaagtcac tgggcaccag ccagaccttg
     6481 ttcgcttcgc ccttggccat ctgcggcagc gtctgcaggt actggtaggc cagcatctcc
     6541 ggggtcggcc gtccggcctt gatcgccgcg aacgtcttct cgatggcctt ggcctggcct
     6601 tgggccacca ggtattgggc ggcccgctcg ccctgggcgc gcagcatgcg cgactggcgg
     6661 tcggcctcag ccgcaagaac cgcggcctgc ttggcgccct cggcagccag gatctgagcc
     6721 tgcttctgtc cctccgcctg tttgatcgac gcctcgcggg agccctcggc ggtgaggatc
     6781 atggcccgct tttcgcggtc cgctctcatc tgcttttcca tcgagtcctg gatcgatggc
     6841 ggcggatcga tgctgcgtag ctccacccga gccacccgca gaccccagcg gccggtggcc
     6901 tcgtcgagca ccccgcggag ctgcccattg atctgatcgc gcgaggtcag cgtctgctcc
     6961 agcgtcatgc cgccgacgac gttgcgcagc gtcgtggtgg tcagctgctc gacaccgacg
     7021 atgtagttgc tgatctggta caccgcggcc tgcgggttgg tcacctggaa gtagacgacg
     7081 gtgtcgatgt tgaccgtcag gttgtcctcg gtgatcaccg gctgcggcgg gaacgacacc
     7141 acccgctcgc gcagatccac ccgcgcccgg atcttgtcga cgaacggcag cagcaatgtc
     7201 agctgacccg acacggtctt gctatagcgg cccagccgtt caatgaccgc ggcctccgct
     7261 tgcgggatca gcgcgatcga cttggccacg ataatggcgg cgaataccac cagcacccca
     7321 accaggatca agccggcgac ggcaccttcc atgacggatt cctcccttgt ttgtccagcg
     7381 ttctctagac aactttttgg acgacggcgg tggcgccgtc gatgtgcacg acggtgacgt
     7441 ggtcgcccgg ttcgtagata tcgtcgtcgt tcagcgggcg cgccgtccag atttcgccat
     7501 cgagtttcac ctggccttca tggcgcgcca cccgatctag caccagcgcg ctcttgccct
     7561 ccagcgcctt tgccggttcg ggcaggccct tgcccgacat gaaatgccgt ttcaatgcgg
     7621 gccgcaccag caccagcaac agcaccgaca cgacgaggaa caccgcgccg tgcacccaca
     7681 gatcgtcgac gagcaggctg gagccggccg cggcaagtgc gccgccaccc agcatgagaa
     7741 ggaagaggtc gccggtgagc gcttccgccc cggcgagggc caatgcggcg attagccaga
     7801 tcagcgcaac gggcatgcgc agagcctagc gcgtagccga cccaatgagc ggttaacaac
     7861 tacactgcga gcatcatgtg gtgcccgagt gtctccctgt cggtgtgggc caacgcctgg
     7921 ctcgcaggcg ctgccgcgcc tgacgacgtc ctcgacgcgt tatcgcaatg ggcgccaagg
     7981 cattccgtca ccgcgtatga ctccgtggcg gccggccgca ccgggttgcc gtggccggac
     8041 ctgaacgacg tgggcgcggt gtcgctgctg cagacggcgc gcacggcggc agggacgggg
     8101 ctgtcggccc ctgcgatcgg cgtggcgctg ccggtgcccg gggatgtgcg cgggctccca
     8161 gccggcactc agttccagcg tgacgcggtg accgcaggtg aggccgtcat cgtcggtcgc
     8221 gatgcatcga ccgcagtggg tttggtcccg gagttcgagt acgacgagtt agaggacgag
     8281 tcggagttcg aaccggagcc gacttcgttg tcgtggaccg tgtattcggt gccggcgggg
     8341 gcgcccgctg agtatttgga tctcggcgag gcggaatacg cgttgcgggc cgccgtccgt
     8401 tcggccgcgg atgcgctcgg cgcaatccgc gccgcgggca ccgatgtggc cgacccacgc
     8461 ggactcgtcg agcaggttct cgaaggcacc cgcggccatc gggtgcccga ccacgcaccg
     8521 tcgcgggcag tgcgcgtgct cgagaacgca gcgcatatcg acgcgatcat caccgtcagt
     8581 tcggggctga tcccgatcgg attacagacg tcgtcggaag tccagatcgc cagcgacgcg
     8641 ttgcgcccac tgtccggcgt tgtgcgctcg gctcggatgg ccgcgatcag cgagattctg
     8701 tattccgcct ggcgctagcc cgctttcgta aaagtgtccc cgcggtaggg cctcgctgct
     8761 cttaccggca tgaacacacc aacagcaccc cggatctgga ccatcgtcac cgcaggcgcg
     8821 atcgcgctag gtgtggcatt gaccgtcaac gcgccggctg cccaagctga acgaatctcc
     8881 gaaagcacga tcaagagcga atgcagggcg gccggtggca cgtactccac cacggtgatc
     8941 aagggcatcg gcgaaaggca ttcgtcgtgc acctacaaag acgaagacgg caataagtgg
     9001 accgacgtct acaccaacgg cgagtaccag ggcaccgatc cggcctaacc gcagttaggc
     9061 gtgcacacca cgccgttgac cgaaaggccc tgcagcggcg gggcatccgg gccaggtacc
     9121 cggaccggtt cgcggccgtg gcgcaactcg tcgatcagat cggcggccag gcgggcgaac
     9181 cgcggatcgg cgttcggcgt cgctgcccgc gcgaatgcca cgtcgaggtc ccgcgcctgc
     9241 tgctgtagct cgtggtccaa gtcccacacg acctcgatgt gatcggcgac gaacccgatc
     9301 gggcagacga tcaccgcctt ggtaccagcc tcggccagcg tcgccagatg gtcggcgata
     9361 tcgggctcca gccacggcac ttgcggcgga cctgagcgcg actgccacac ctggtcgtag
     9421 tcgtcgtagc cggccgccgt ggctaccagg cgagccgcat gggacacctg gcggctgtat
     9481 aggtccgcac cgcaacggtt ttgggccacg accgggatgg aatgcgcggt gaagaccagc
     9541 cgtgcgtcgt ccggcacggt ctgcgccgat tcgccgatgg cttcggtgaa catctccacg
     9601 aacagcggat ggtcgaagta ctgacgcagt ttgaccaatt ccggcgcgcc ctcgccggct
     9661 gccgcgcggg ctcgggcgat gtcctcgacg tactgggtgc agctggagta accgccccac
     9721 gccgaggtgg tgaacacggc tgcgcggcga atgccgttgt cgcgcatggt cgcgacggtg
     9781 tcctcgacat acggttccca gttgcggttg ccgaagtaga ccgg
//
LOCUS       DASRJA010000135         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_173717,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000135 DASRJA010000000
VERSION     DASRJA010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173717"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VFC01_06145"
     CDS             complement(<1..709)
                     /locus_tag="VFC01_06145"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZN79256.1"
                     /translation="MRIVFAGTPEPALPSLRRLIDSPHHDVVAVLTRPDAAKGRHGKP
                     SPSPVAHLALEHDIPLLRPTRPNSDEFIAELTELAPEACAVVAYGALLSERLLAVPTH
                     GWVNLHFSLLPAWRGAAPVQASIAAGDTVTGATTFQIELALDSGPVYGVVTETIRPTD
                     TAGDLLERLSISGAALLETTLDGIGDRSLSAVPQPADGVSVAPKITVESARVRWDLPA
                     HVVDRQIRAVTPNPGAWT"
     gene            complement(729..1265)
                     /locus_tag="VFC01_06150"
     CDS             complement(729..1265)
                     /locus_tag="VFC01_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZN79257.1"
                     /translation="MVRFLLILVLALAVLVLIGFVMGYNKLRSADVRVAEALSGIDVE
                     LTRRASLIPSLVHTVETFAAHEKGILDHVTNARAALTSATTGKSVAQRSAAERELDTA
                     LAPLLALGQSYPQLNSSNNFLNLQDNLADTENKLAFARQYYNDAVATLNRLISTIPWM
                     FVAPMSGVSEREYYQTPR"
     gene            complement(1259..3067)
                     /locus_tag="VFC01_06155"
     CDS             complement(1259..3067)
                     /locus_tag="VFC01_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HZN79258.1"
                     /translation="MGRVFKWLIPLALIAFGLLWPLVFRGGAQAAEADDPVVFSNYKA
                     DFIVDRNGDMNAVETITGEFPGGRHGIFRWFDITNQNVSGVRQKPEITSILLDGKPVS
                     YQMLWEGGERFRVAKIGDPDKYLSYGTHVFEIRYTVPGVLDPGTTGAERTFAAATGPS
                     AASPSVFFWNVIAPSESRIGRADISVTLPGDVGRAQCSVGYGVGRRCEDLTVSGNTVK
                     LSTDYLLPRTPVTVRAGVDVPTPAQTSVPWPYTWDRILGQSLTGVVWIAALTVAFGLG
                     AFLWYRTTVEPSPGFPLQYAPPPGLGPVQTEYIRTESVPKNGLTATLFYLAERRLIDL
                     RQVNEKQWNVRGIAEPGAWADVDPVSVAVGSVLKVMKAGTEFEAKKTVKAGQRLDKAK
                     RDMAKAVEKWAFDSGLMVKRKKEMWVRTANAIAFILMLCGFFRWAFPTTMWALPFVAF
                     FLFSIRSWADGVGTRRTETGRELWSRAGGFHRMLATDSSETRFDFGARKDLYSAYVPF
                     AVAAGVAALWAKKYETSTGTPAPQPDWYNSSSATTWGCAGGSSGPSFDSFESALSSSI
                     GAYTASQSSSSSSGGGGGGFSGGGGGGGGGGGSGSW"
     gene            complement(3105..>3603)
                     /locus_tag="VFC01_06160"
     CDS             complement(3105..>3603)
                     /locus_tag="VFC01_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559777.1"
                     /note="binding of PriA to forked DNA starts the assembly
                     of the primosome, also possesses 3'-5' helicase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosome assembly protein PriA"
                     /protein_id="HZN79259.1"
                     /translation="LGRQDLRAAEDTLRRWMAAAALVRPRGDGGTVAVVAESAIPTVQ
                     SLIRWDPVGHADGELDARAEVGLPPAVHIAAVDGVPAAVGALLDTAELPETAQLLGPV
                     DLPTGARRPPGVPADSPVSRMLVRVPRDGGLALAAALRRATGVLSARHDQQPTRVQID
                     PLHIG"
BASE COUNT          607 a         1224 c         1197 g          575 t
ORIGIN      
        1 ttgtccatgc gcccggattc ggtgtgacgg cccggatctg ccgatcgacg acatgcgcgg
       61 gcaggtccca ccgcacgcgg gccgattcga ccgtgatctt cggtgcgacg ctgacgccat
      121 cggcgggttg cggcaccgcc gacaatgacc tgtcgccgat gccatccagc gtcgtctcca
      181 gcagcgccgc acccgaaatc gacagtcgct caagcagatc gcccgcggtg tccgtgggcc
      241 ggatcgtctc cgtcaccacg ccgtagaccg ggcccgagtc gagcgccagt tcgatctgaa
      301 acgtcgtcgc cccggtcacg gtgtcaccgg cggcgatcga agcctgcacg ggtgccgcgc
      361 cccgccatgc cggcagcagc gagaaatgca ggttgaccca accgtgggtg ggcaccgcca
      421 gtagccgctc ggacagcagc gcgccgtagg cgacgaccgc gcacgcctcg ggtgccagct
      481 cggtcagttc ggcgatgaac tcgtcggaat tcggccgcgt cggacgcaac agcgggatgt
      541 cgtgttcgag cgccaggtgg gccaccgggg atggcgacgg cttgccatgc cgacccttcg
      601 cggcgtcggg gcgggtcagc accgcgacca cgtcgtggtg tggcgagtcg atcagcctgc
      661 gcagcgacgg cagcgcgggc tcgggggtgc cggcgaagac gatgcgcacc gcgccagtgt
      721 agggatgcct accgcggcgt ctggtagtac tcccgctcgg acacccccga catcggtgcg
      781 acgaacatcc acgggatcgt gctgatcagc cggttcagcg tggccaccgc gtcgttgtag
      841 tactgccgcg cgaacgccaa cttgttctcg gtgtcggcga gattgtcctg cagattcaag
      901 aagttgttcg acgagttcag ttgcggataa ctctggccga gtgccagcag cggcgcgagc
      961 gcggtgtcga gttccctctc ggccgcactg cgctgggcca ccgacttacc ggtcgtcgcc
     1021 gacgtcagcg ccgcacgcgc gttggtcaca tgatcgagga tgcccttctc gtgggcggcg
     1081 aacgtctcca cggtgtgcac caggctggga atcagggacg cccgccgcgt cagctccaca
     1141 tcgatcccgg acagggcttc ggcgacacgg acgtcggcgg aacgaagctt gttgtagccc
     1201 atcacgaaac cgatcagcac cagcaccgcc agtgccagca ccagtatcag caggaatctc
     1261 accatgatcc gcttcctcct ccaccaccgc cgccaccgcc gccgccactg aagccgccgc
     1321 caccaccgcc ggacgatgac gacgaggact gcgacgcggt gtaggcaccg atcgacgacg
     1381 ataacgccga ttcgaagctg tcgaaactcg gcccactgga gccgccggca catccccatg
     1441 tggtggccga cgacgagtta taccaatcgg gttgcggcgc gggcgttccc gtcgacgtct
     1501 cgtacttctt cgcccatagc gccgccacac cggcggccac cgcgaacggc acataggcgg
     1561 agtagaggtc cttgcgcgct ccgaagtcga agcgggtctc tgacgagtca gtcgccagca
     1621 tgcggtggaa tccgcccgcg cgcgaccaca attcgcggcc cgtctcggtg cgccgggtgc
     1681 cgacgccgtc ggcccacgac ctgatcgaaa acaggaagaa cgcgacgaac ggcagggccc
     1741 acatcgtggt cgggaacgcc catcggaaga agccgcacag catcaggatg aacgcgatcg
     1801 cgttggcggt gcgcacccac atttccttct tgcgcttgac catcagcccg ctgtcgaaag
     1861 cccatttctc gaccgccttt gccatgtctc gcttggcttt gtcgagtcgc tgcccggcct
     1921 tgacggtttt cttggcctcg aactcggtgc cggccttcat gactttcaat accgaaccga
     1981 ccgcgacgct gaccggatcg acgtcagccc acgcgcctgg ttcggcgatt ccccggacgt
     2041 tccactgctt ttcgttgacc tgccgcagat cgatcagcct gcgttcggcg aggtagaaca
     2101 gcgtggcggt gagcccgttc ttcgggacgg actcggtgcg gatgtactcg gtctgcaccg
     2161 ggccgaggcc cggcggcggc gcgtactgca gcgggaatcc gggcgacggt tcgactgtgg
     2221 tgcggtacca caggaacgcg ccgagcccga aagccactgt cagggctgcg atccacacca
     2281 caccggtcag cgactggccc aggatgcggt cccaggtgta gggccacggc acactggtct
     2341 gcgccggcgt aggcacgtcg acgccggcgc gcacagtgac cggtgtgcga ggcaggaggt
     2401 agtccgtcga cagcttgacg gtgttgccgc tgactgtcag gtcttcgcag cgacgtccga
     2461 cgccgtaacc gacggagcac tgcgcgcggc cgacgtcgcc gggcagtgtg accgagatat
     2521 cggcgcgtcc gatccggctt tcggacgggg cgatgacgtt ccagaagaac accgagggcg
     2581 aggccgcgga cggaccggtg gctgcggcga atgtcctttc cgcgccggtg gttccggggt
     2641 caaggacacc agggacggtg taacggatct cgaagacgtg ggtgccatag gacaggtact
     2701 tgtccggatc gccgatcttg gcgacacgga accgttcacc gccctcccac agcatttggt
     2761 atgagaccgg cttcccgtcg agcaggatcg acgtgatctc tggtttctgc cgtactccgg
     2821 agacgttctg gttggtgatg tcgaaccaac ggaaaatacc gtgccggcca ccggggaatt
     2881 ccccggtgat ggtctccacg gcgttcatgt cgccgttgcg gtcgacgatg aagtccgcct
     2941 tgtaattgct gaagacgaca gggtcgtcgg cttcggcggc ctgcgcgcca ccgcggaaca
     3001 ccagcggcca cagcagccca aaggcgatga gcgccaacgg gatcagccac ttgaacaccc
     3061 gccccatcgc cacctcctga tcggcgcgtg tcgccgtgtc cggattaccc tatgtgcaaa
     3121 gggtcgattt gcacacgagt tggctgttga tcgtgccgcg cgctgagcac gccggtggcc
     3181 cgtcgcagcg ccgccgcaag cgccagcccg ccgtcgcggg gcacccgcac caacatccgg
     3241 ctcactggac tgtcggccgg gacgccgggc ggcctgcggg caccggtggg caagtcgaca
     3301 ggcccgagca gctgcgccgt ttcgggcaat tcggcggtat ccaggagtgc acccacagcg
     3361 gcgggaacac cgtcgaccgc ggcgatgtgc acggcgggcg gcaggccgac ctcggcgcgg
     3421 gcgtcgagct ctccgtccgc gtggcccacc ggatcccacc ggatcaacga ctggacggtc
     3481 gggatcgccg actcggcgac caccgcgacg gtgccaccgt caccacgggg ccgcaccagt
     3541 gcagctgcgg ccatccagcg ccgcagggtg tcctcggcgg cgcgaagatc ctggcggccg
     3601 agc
//
LOCUS       DASRJA010000136        23074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1022111,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000136 DASRJA010000000
VERSION     DASRJA010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 23074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23074
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022111"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /gene="lnt"
                     /locus_tag="VFC01_06165"
     CDS             <1..616
                     /gene="lnt"
                     /locus_tag="VFC01_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZN79260.1"
                     /translation="RDRTGGIYKSAVLIGPNGSRGAYSKTRLVPFGEYVPLRPLLGWA
                     TSHTKAAAEDRRRGDGPAVLHADSLAVGPLISFEATFSDLPRHEVRLGAELLAYQSST
                     STYQRTWAQPQLASMAAVHAVEVGHPAVHAGLSGVSSAFDARGRELGWLPASSRGVLV
                     VEVPLGSRTTCYDRLGDWVIGLALLMVTASCAQSARRSWTGVRR"
     gene            complement(561..1007)
                     /locus_tag="VFC01_06170"
     CDS             complement(561..1007)
                     /locus_tag="VFC01_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZN79261.1"
                     /translation="MSGYTTIVVGTDGSDSSLRAVDKAGQIAAGSDAKVVVATAYFPQ
                     SEDQRAADVLKDEGYKMAGNAPIYAILREARDRAQQAGATNVEEKAVVGAPVDALVDL
                     AEEVNADLLVVGNVGLSTIAGRLLGSVPANVARRSKTDVLIVHTTP"
     gene            1346..2884
                     /locus_tag="VFC01_06175"
     CDS             1346..2884
                     /locus_tag="VFC01_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79262.1"
                     /translation="MDLSTRSPRLARVQQRARVRAGRFDPAIVAVFAAVVCAAGAARP
                     SLWFDEAATISASTRSVPDLWRLVHNIDAVHGLYYLVMHGWFTVFPVTEFWSRLSSCL
                     AVGIAAAGVVVVAKQFSTRTVAVCAGVVFAILPRVTWAGIEARSYALTAAAAVWLTVL
                     VVTAARRNAAALWPLYGLALVVSTLLNVFVVLMVLVHAVVVPVVASKRATVVWWAATS
                     TVAVLVLAPFLAFCRNQIAQVRWISPLDAGTVLDIAKEQYFDHSVSFAVLTVVVLVAV
                     LVARRQWDPSNRQLAAVAVAWIVLPTAVLLVYSVVQQPIYYPRYLCYTSPAMALLIAV
                     CTVALFRTRERVTAMLAAFALAATPNYLLNQRGPYAKEGMDFSQVADVITAHAAPGDC
                     LILDNTTAWKPGPIRPLTAARPAAYEKLVDPGRGLRGSQRNRLWDAHLGIWGVADRVR
                     LCTVLWTVSERDPTVPERQSGPGLDPGVRLKRAPAYQVPQHMGFHIVERWQFSFAQVV
                     KSTR"
     gene            2901..3563
                     /locus_tag="VFC01_06180"
     CDS             2901..3563
                     /locus_tag="VFC01_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884392.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HZN79263.1"
                     /translation="MAKRSAAEFVPDSHNLKTLSDAAEGCKGCDLYLDANQTVFGEGA
                     PDADMMLVGEQPGDQEDKAGAPFVGPAGKLLDKALVAAGVDRDRLYVTNAVKHFKFTL
                     PERGKRRIHKTPSRTEVVACRPWLFAEMMSVEPDVVVLLGATAAKSLMGSDFRLTAHR
                     GEALHLPTTDDVTNLDFDPEIVVSAHPSSVLRGPPEDREKAFHALVSDLRFAAKRLKR
                     KR"
     gene            3572..4309
                     /locus_tag="VFC01_06185"
     CDS             3572..4309
                     /locus_tag="VFC01_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN79264.1"
                     /translation="MQCVVDRVVRLPDGRSMGYAQYGSDGGFPIVNAHGGLACRLDVA
                     AANTVAAEADVRLISPDRPGIGLSDPQPGRTVLDWANDVADLFDRIDVDRFAVMGWSM
                     GGQYAAAVGHAMPHRVTRLAIIAGALPLTDPGVFDELPTMDWVLTGMSRRAPWLAAHS
                     GSGSCGSQQVPRPHCTDDWPRASWGRLTARSSAMKASTRSPGCPAKPYGNRPAPSKSI
                     GHGCGRGVSLPRTSRCQSTSGRGPTTN"
     gene            complement(4430..5221)
                     /locus_tag="VFC01_06190"
     CDS             complement(4430..5221)
                     /locus_tag="VFC01_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZN79265.1"
                     /translation="MVQIACCQIEPVVGDLAANAAVIEAQIREAVGVGADVIVLPELA
                     TSGYMFADADEARATALRPTDFANWSASVDDALVIGGFCELGDDGRLYNSAVMVDADG
                     VVASYRKTHLWDREKLIFTPGDALPPVVKTKHGAIAVMICYDLEFGELTRRVALDEAE
                     LIAAPVNWPLFPRPEGEHPGEVITAMSTARTNKVAIACCDRAGVERGQQWTEGTAIID
                     PDGWVVAASGPGIAMAVADIDLAATHDKTLTEHVHLFDDRRVDLY"
     gene            5341..6753
                     /locus_tag="VFC01_06195"
     CDS             5341..6753
                     /locus_tag="VFC01_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HZN79266.1"
                     /translation="MESPAAARIEKRSIDYVPENERHGKVSHQGPFWFVGNFQPFTLA
                     LGFVGPSLGLSLWWSIVAGIAGIAFGTLFMAFHATQGPVLGLPQMIQSRAQFGFRGVL
                     LPLIGTLFTFVGFNVVDVVIIKSGLQSIFGWNPVVVAAAITVIAALLAIFGHDLLHKS
                     FRVLFWISLPLWIILTVSVLFGGVTGGPAAPGGFSWVGFLAQFGVAASYNITYAPYVS
                     DYSRYLPRDTKPTAIIASVFVGAAGSPAWLIPLGAWMATYLGASDALAGINETGNSAI
                     SYLGSVLAIVSTLVLVATMGLNAYSGMLTVVTALDSLRPVKPTRRLRVVTIIVLAVAW
                     FVMSLLLTNATTALNTTLLIMLYLLVPWTAVNLTDYFFVRRGHYAIADLFIPNGIYGA
                     WSWRGLTAFVVGIAAEIPFVVLPFFIGPAAAAMGDVDIAFIVGLLVSGVVYVLLTRSL
                     DVSHELELIESHPELADPIS"
     gene            complement(6758..7057)
                     /locus_tag="VFC01_06200"
     CDS             complement(6758..7057)
                     /locus_tag="VFC01_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79267.1"
                     /translation="MNAKRIGAGAAVVSALAFTAIGMGAGVASADQPLPSTPGMTWKL
                     DRGHGHGHGGDWDWGDGDWNGGWNGGWQAPPAPCGAGYWVPPAVWQWVPPAAWGC"
     gene            complement(7384..7815)
                     /locus_tag="VFC01_06205"
     CDS             complement(7384..7815)
                     /locus_tag="VFC01_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN79268.1"
                     /translation="MAKLELSRELSVTPEEAWAHASDLAHLGDWLVMHQGWRGEVPAE
                     LTTGATFVGVAGAKGLRNRVTWTVREVEPPRMLEMTGDGVGGTKYALRMTVSPTASGA
                     KFTFRLDLGGRPLFGPIGAAAARAVKGDIERSIQRFEALYS"
     gene            complement(7827..8897)
                     /locus_tag="VFC01_06210"
     CDS             complement(7827..8897)
                     /locus_tag="VFC01_06210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN79269.1"
                     /translation="MDGPPLRPVDAAAQRDAMTRRLHRRSIVNGQITLPAVPGMIDQY
                     MSMCESLFATVGRPFTAEQLAHVKSVLEGQLAEAYANSPRSNIVISFDAPVGTVLNYH
                     VKAEWWTVEGAYANWIGTREPPLFGTEPDARIWALATQAPDAATHRVLDIGGGTGRNA
                     LALARRGHPVDVVEMTPKFADIIRTDAERESLNVRVIERDIFSSDEDLRRDYWLILLS
                     EVVSDFRTTQRLRAVFGLATHCLVAGGQLVFNAFLAKPGYTPDDAARELGQQCYTSIF
                     TSQELSAAASGLPLQLVSDDSVYLYEKSHLPQGAWPPTSWYADWVSGRDVFDLPREEI
                     PIEMRWLVYQKPNWNHGNRSIT"
     gene            complement(8957..9027)
                     /locus_tag="VFC01_06215"
     tRNA            complement(8957..9027)
                     /locus_tag="VFC01_06215"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8993..8995),aa:Cys,seq:gca)
     gene            complement(9089..9850)
                     /locus_tag="VFC01_06220"
     CDS             complement(9089..9850)
                     /locus_tag="VFC01_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HZN79270.1"
                     /translation="MPTTPTAYPEIATREEWLAARKKLLAREREVTHLRDAVNAERRR
                     LPMVKVEKDYVFEGPDGELRLLDMFEGRPQLYIHHFMWIDAIDKGCPSCTMAADLTFT
                     EKDRALLNAKGVTFAAVSRAPYASIARYNAEHGWTFPWYSSRDNDFSYDYHVTLDPKR
                     TPIEYNYKSLDELHAAGFGDDALRGDWPGASVFLRRGDDVFHTYSAFARGLDHSAPGY
                     PFLDLTPYGRQETWEDSPAGWPQGADAVGVPMDEQ"
     gene            9943..10671
                     /locus_tag="VFC01_06225"
     CDS             9943..10671
                     /locus_tag="VFC01_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZN79271.1"
                     /translation="MQVWLADPPFFGGPGQGARQIVELFVAFGLTSLIGLEREIQGKS
                     AGVRTQTIVGTAAALILLVSKYGFSDVLQDGLVIVDPSRVAAQIVSGIGFLGAGIIIF
                     RRGSVHGLTTAAAVWESAAIGMAAGAGMLLLAVTVTAMHFLIIVGFMPLAKRLSARLA
                     GSVTMHITYEEGRGVMSRLLQACDRRQWQLTDLAADAAGERVSPGSTGVMLTLSGRGI
                     LTAPTVLAGIDGVTAIRQLDDEPD"
     gene            10756..12310
                     /locus_tag="VFC01_06230"
                     /pseudo
     CDS             10756..12310
                     /locus_tag="VFC01_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383146.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
     gene            12310..13035
                     /locus_tag="VFC01_06235"
     CDS             12310..13035
                     /locus_tag="VFC01_06235"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HZN79272.1"
                     /translation="MATVTIGLICAIPQELAHLRANLADSRSEEAAHTVFDTGTIDGH
                     DVVLAGSGMGKVNAALVTTLLADRFECRTVVFSGVAGGLDPRLEIGDIVVADFVIQHD
                     AGVLENERVRTYQPGYAPVINPTDRLGYPVDPGLLDRVRKRLEGLSIPGQIVYGTVLS
                     GDQYLNCETTRERLLRELKGQAIEMEGGAVAQVCEAFGIPWLVVRALSDLAGGNALFD
                     FTAFVDQAAAASATILRRLLPVL"
     gene            complement(13001..14407)
                     /locus_tag="VFC01_06240"
     CDS             complement(13001..14407)
                     /locus_tag="VFC01_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN79273.1"
                     /translation="MTDTATIIRGSWRELLGPKNLGASTVLAGGVALYATNEFLTISL
                     MPSAVADIGGQRFYAWVTTVYLVASVVAATTVHSLLMRLGPRWSYLLGLSVFGVGSLT
                     CAAAPTMEIMLVGRTVQGAAGGLLAGLGYAVINTALPSTLWTKASALVSAMWGVGTLV
                     GPAAGGLFAQFSSWRWAFGILVVLTAAMAILVPIALPARRAADVEDTRTPIPVWSLLL
                     LGAVALAVSVAGIPHDVWATTALLALGAALVAVFAYVDRRLTASVLPPSTFCSGPLKW
                     IYLTLGVLMAATMVDMYVPLFGQRLAHLTPVAAGFLGAGLAIGWTVGEISSASLSSER
                     VIRRTVAVAPLVMATGLALGALTQFADAALWLVAVWAAALMITGAGVGIAWPHLSAWA
                     MCCVDDPAEGPAAAAAINTVQLICGAFGAGLAGVAVNLTDTGDATPARWLFAVFAVLA
                     AFGAVASFRAQAAGAAGS"
     gene            complement(14426..15316)
                     /locus_tag="VFC01_06245"
     CDS             complement(14426..15316)
                     /locus_tag="VFC01_06245"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN79274.1"
                     /translation="MELRHLRSFVAVAEELHFGRAAERLHIAQSPLSQQIQRLERQVG
                     ATLFERNRRKVELTEAGHAMLRHAREALAQADLAENAARTAAAGRAGLLRVGFLGSAA
                     LAILPRIVPPWRATAPDVALRLVEGSSGEHIRAVHDQRLDVAFVRPPATTAGLVVYPV
                     WHEPVVAVLPATSDLSRRERLRLRDLRDQPFVLFPRDSAPDFHDELTRACTRVGFTPA
                     VAYECTAMPTVVGLVASGLGVALVPRSINSIGLDGVVYRELADAHPEARIAMVVSTTN
                     DRPAVRHFIDSVIRLDLNSG"
     gene            15388..16872
                     /locus_tag="VFC01_06250"
     CDS             15388..16872
                     /locus_tag="VFC01_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HZN79275.1"
                     /translation="MNPRNEADVIVVGAGSAGAVVASRLTENPGRTVLLIEAGPDFGT
                     GVETQPQDIIDAEDSTATAFDWQHTGTPSGLHREIPLYAGKVVGGSSATNNVMALRGD
                     PAVYDSWAIDGWSFKDLLPAFCRLERDMDYSDRAWHGDAGPVRIRRHVAGPVHAAFLN
                     VAATQGHPSVDDHNRPSAVGAGALPLNQINGVRQSTTLTYLRVARTRPNLWVRANATV
                     DRVLLDHGSTRGVRLANGAEIHADSVILSAGALGSPAILLRSGIGPAEDIRTLGIPVV
                     ADRRGVGENLHDHPLLRMTFSTDGERARPVRQTVLTARTGTDAAPDLQLFPSGPTPAD
                     DGAVLTMLVALLTPRSRGRMRLTAADPLAPLDIDPGHLEDRADLPRVVAGVELARELL
                     TSPPLARYIQAAAPDTRSLMSEQGVNLRSAIVDQLNTYHHPVGACRMGPPDDDMAVVD
                     PAGSVYGVTGLSIVDASIFPTIPSANTNLPTLMAAEHLAPALTT"
     gene            complement(16865..19015)
                     /gene="uvrB"
                     /locus_tag="VFC01_06255"
     CDS             complement(16865..19015)
                     /gene="uvrB"
                     /locus_tag="VFC01_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019731486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZN79276.1"
                     /translation="MAFATEHPVLAHSEYRAVDEIVRVGGRFEVVSPHAPAGDQPTAI
                     EELERRIKAGERDVVLLGATGTGKSATTAWLIERLQRPTLVMAPNKTLAAQLANELRE
                     MLPHNAVEYFVSYYDYYQPEAYIAQTDTYIEKDSSINDDVERLRHSATSSLLSRRDVV
                     VVASVSCIYGLGTPQSYLDRSVELQVGQEVPRDALLRLLVDVQYNRNDLAFTRGAFRV
                     RGDTVEIIPSYEELAVRIEFFGDEIESLYYMHPLTGDVVRKVDSLRVFPATHYVAGPE
                     RMAHAISTIEAELEERLSELEGQGKLLEAQRLRMRTNYDVEMMRQVGFCSGIENYSRH
                     IDARPAGSAPATLLDYFPEDFLLVIDESHVTVPQIGGMYEGDISRKRNLVDFGFRLPS
                     AIDNRPLTWEEFADRIGQTVYLSATPGPYELSQTGGEFVEQVIRPTGLVDPQVIVKPT
                     KGQIDDLIGEIRKRTERDERVLVTTLTKKMAEDLTDYLLEMGIRVRYLHSEVDTLRRV
                     ELLRQLRLGDYDVLVGINLLREGLDLPEVSLVAILDADKEGFLRSVRSLIQTIGRAAR
                     NVSGEVHMYADNITDSMREAIDETDRRRAKQIAYNEANGIDPKPLRKKIADILDQVYR
                     EADDVEVGGSGRNASRGRRAQGEPGRAVSAGIIEGRDTTNMPRAELADLIKDLTEQMM
                     AAARDLQFELAARIRDEIHDLKKELRGMDAAGLK"
     gene            complement(19061..19840)
                     /locus_tag="VFC01_06260"
     CDS             complement(19061..19840)
                     /locus_tag="VFC01_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZN79277.1"
                     /translation="MPSVIVDQQDHVLRIGLNRPAKRNAFNVEMLDELGRAYERLERD
                     DDLRVGVLYANGDHFTAGLDLAEVGPRLMEGSLDWPDDARNPWRNDGRPWTKPVVAAV
                     QGWCLTLGIELLLAADVRIASADARFAQLEVQRGIYPFGGATTRLPREAGWGNAMRWL
                     LTGDEFDAPEALRIGLVQEVVAPGDQLDRAVELAERIATRSAPLAVRTTLAAAQRANR
                     EGEQAAEGRFVDDVVALFRTKDGAEGMLSFIERRPARFIGA"
     gene            19861..20457
                     /locus_tag="VFC01_06265"
     CDS             19861..20457
                     /locus_tag="VFC01_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN79278.1"
                     /translation="MTVITSDSRKRMIQSAALLFREHGYSGTGFRDVIEHSGAPRGSI
                     YHHFPGGKEQLAADTVAWAAAVIERRIERAARAGNPLVALSMFIDAWREVLEDSNFRA
                     GCPIVAVAAEADAGSTAIAAAAVAFGRWQDLIAHALLRAGVGRTDARRLATLVIASIE
                     GAILLCRARRDIRPLRDVHRALEATLRTAISWHGRPVE"
     gene            20483..20989
                     /locus_tag="VFC01_06270"
     CDS             20483..20989
                     /locus_tag="VFC01_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HZN79279.1"
                     /translation="MHAPKHETFDLVARTNTDPKGIVRAVDVYTVEPWGLYMARPTPG
                     RAQFHYMESWLLPSLSLRATVFHFNPGYELDQDYYLDVGLYTAGATAWHAEDHYLDLV
                     VRTDVGVELSDVDELLTAVRHGLLTPETGEHAVQTAVSAIEGLSRHDYDLNRWLADNG
                     MALTWRGA"
     gene            21019..21567
                     /locus_tag="VFC01_06275"
     CDS             21019..21567
                     /locus_tag="VFC01_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HZN79280.1"
                     /translation="MTLKEYRNKRRSGRSPEPRGRRRRARKQPRFVIQHHAASTDHYD
                     FRLEIDRVLVSWAVPKGPSTNPKDKRIARRTEDHPMEYETFEGIIPKGEYGAGRVIVW
                     DRGTYTTERDMAEALERGHLSFQLDGEKLHGGYALTRLRQGKDEAWLLVKKADDDADA
                     RRKPVRSQPESVLSGRTLDELS"
     gene            21564..22511
                     /gene="ligD"
                     /locus_tag="VFC01_06280"
     CDS             21564..22511
                     /gene="ligD"
                     /locus_tag="VFC01_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZN79281.1"
                     /translation="MKVPQWRAPTLATLTNERFSDPEWIFERKLDGVRGLAFRDGKHV
                     RLMSRNRLSLNGTYPEIVEALTSQDTPRFVADGEIVAFEGRRTSFGRLQGRSGITDPD
                     QARASGIPVFYYVFDLLHLDGNDTTQLPLIERKRLLRNEFQFADPLRYTTHRVKDGEA
                     AYRAACERGDEGVIAKHADSPYEGGRSTNWLKFKCSRDQEFVVVGYTSPKGSRIGLGA
                     LLLGYHDDGDLVYAGKVGTGFDTATLRGLHERLSPTDQKAPPITKGLAREPGAHWVRP
                     TLVAQVAFSEWTRDGKLRHPRYVGLRTDKSPDDVVRETR"
     gene            22511..>23074
                     /locus_tag="VFC01_06285"
     CDS             22511..>23074
                     /locus_tag="VFC01_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZN79282.1"
                     /translation="MPVKVEITHPDRVLFPDDGITKGDVVDYYAEVAAVMVPHLKGRP
                     LTLWRYPRGIDQHGFVQQDFGGAMPDWMGAAEVAKEGGTVVHPVADRRDALVWLANQN
                     CVTPHAWLSRRDRLDVPDLIVIDLDPTTTDFAPVRAAARACADVLDDLGLTSYLKTTG
                     SRGLHVVVPISRGPDYDAVRQFARDVAD"
BASE COUNT         3877 a         7639 c         7550 g         4008 t
ORIGIN      
        1 ccgtgaccgc acgggcggaa tctacaaatc agcggtcctc atcggaccga acgggtcgcg
       61 cggcgcgtac tcgaagacgc ggctggtgcc gttcggcgag tacgtgccac tgcggccgct
      121 gctcggctgg gcgacttcac acaccaaggc cgcggccgag gaccgtcgtc gcggtgatgg
      181 accggcggtg cttcacgcgg actctctggc cgtcggacca ttgatcagct tcgaggcgac
      241 cttctcagac ctgccgcggc acgaggtgcg cctcggtgcc gaacttctcg cgtatcagag
      301 ctcgacgtcg acataccaac gcacttgggc ccaaccgcaa ttggcgagca tggccgccgt
      361 gcacgctgtc gaagttggac acccggcggt gcatgccggg ttatccggcg tcagttcggc
      421 gttcgacgca cggggccgcg aactcgggtg gttaccagca tccagtcgcg gtgtgttggt
      481 cgtggaggtt ccgctgggat cgcggacgac ctgctacgac cgtctcggag actgggtgat
      541 cggattggcg ttgctgatgg ttacggcgtc gtgtgcacaa tcagcacgtc ggtcttggac
      601 cggcgtgcga cgttagcggg cactgaaccg agcagccgcc cggcgatggt gctcagcccg
      661 acattgccca ccaccagcag atcggcgttg acttcctcgg cgagatcgac aagtgcgtcc
      721 accggcgcgc ccacgaccgc cttctcctcg acgttcgtgg cgcccgcctg ctgtgcgcgg
      781 tcgcgggcct cgcgcaggat cgcatagatc ggcgcattgc ccgccatctt gtagccctcg
      841 tccttgagca cgtctgcggc gcgctgatcc tcgctctgag ggaagtaggc cgtcgccacg
      901 accactttcg catccgaccc ggcggcgatc tggccggcct tgtccaccgc gcgcagcgac
      961 gaatccgatc cgtccgtgcc gaccacgatg gtcgtatagc cgctcatcca atccctccaa
     1021 gtgtcagttg caacgccacc cgagacagta acccgttgct tgttgaccag gggcgcaatt
     1081 cacgacaccc gatggtaacc ggcggataca cagcaagcgc acctgaagtg atcgaaccga
     1141 ccccgtcgtg cgcccgaacg cccgtgtcgc aacggcattg ccatcgtgat ccagatcaac
     1201 tcaccgaacg ggccacggcc gcaacggaag tcgccgccaa tctctacggc gccgtcaaat
     1261 aggcggcagt agcgggcttt agcgagcgcg cagcgtaggc acgggttagg gttttgccgt
     1321 caccgaaagg ggtcaggtcg accaggtgga tctttcaacc agatcgccgc gccttgcgcg
     1381 cgtccaacag cgagcgcggg ttcgggccgg acggttcgac cccgcgattg tcgccgtgtt
     1441 cgccgccgtg gtgtgcgcgg cgggcgccgc ccgcccgtcg ctgtggttcg acgaggcggc
     1501 gacgatctcc gcgtcgacgc ggtcggtgcc cgacctgtgg cggctggtcc acaacatcga
     1561 cgccgtgcac ggcctgtact acctggtgat gcacggctgg ttcaccgtct tccccgtgac
     1621 cgaattctgg tcgcggctgt cgagttgcct ggccgtcggg atcgccgcgg caggcgttgt
     1681 cgttgtggcc aaacagtttt cgacgcgcac ggtcgcggtc tgcgcaggcg tcgtcttcgc
     1741 gatcctgccg cgcgtcacct gggcgggcat cgaggccagg tcctatgcgc tgacggctgc
     1801 ggccgcggtc tggttgacgg tgctggtggt cacggccgcc cgacggaacg cggcggcgct
     1861 gtggccgctt tacgggctgg ccttggtggt gtcgaccctg ctcaacgtgt tcgtcgtgtt
     1921 gatggttctg gtgcatgcgg tcgtggtgcc ggtcgtggcg agcaagcggg cgacggtggt
     1981 gtggtgggcc gcgacatcga cggtggcggt gctggtcctc gcgcccttcc tggcgttctg
     2041 ccgcaaccag atcgcccagg tcagatggat ttcgccgctc gatgcgggca cggtgctcga
     2101 tatcgcgaag gagcagtact tcgaccacag cgtctcgttc gctgttctta ccgtcgtggt
     2161 cctggtggcc gtgttggtgg cgcgacggca gtgggacccg agcaaccggc aactcgcggc
     2221 cgttgcggtg gcgtggatcg tgctgccgac cgcggtgttg ctggtgtact cggtggtgca
     2281 acagccgatc tactacccgc gatacctctg ctacacctcg cccgcgatgg ccctgctgat
     2341 cgcggtctgc accgtggccc tgtttcgaac ccgcgaacgt gtcaccgcaa tgctggccgc
     2401 gttcgcactc gcggccaccc cgaactacct gctgaaccag cgcggcccgt atgccaagga
     2461 aggcatggac ttcagccagg tcgccgacgt catcaccgcg cacgccgccc caggcgactg
     2521 cctgatcctg gacaacacga cggcgtggaa gcccggcccg atccggccgc tgacggcggc
     2581 ccgacccgcg gcctacgaga agctcgtcga ccccggccgc ggattgcggg gctcgcaacg
     2641 caaccgcttg tgggacgcac acctgggcat ctggggcgtc gccgaccggg tacggctgtg
     2701 caccgtgttg tggacggtgt cagagcgcga cccgaccgtg cctgaacgcc agagcgggcc
     2761 gggattggat cccggagtcc gactgaagcg ggcgccggcc tatcaggttc ctcagcacat
     2821 gggttttcac atcgttgaac gctggcagtt cagcttcgcg caggtggtga agtcgacccg
     2881 ataaggggta atcgactcac atggctaagc gcagtgccgc agagttcgtg cccgacagcc
     2941 ataacctcaa gacgctttcg gacgccgcgg agggttgtaa gggttgcgat ctgtacctcg
     3001 acgccaatca gacggtattc ggcgagggcg cgccggacgc cgacatgatg ctcgtcggtg
     3061 aacaacccgg cgatcaggag gacaaggccg gggcgccgtt cgtggggcct gcaggcaagc
     3121 tgctcgacaa ggcgctcgtc gcggccggcg tcgacaggga ccggctgtac gtcaccaacg
     3181 cggtgaagca tttcaagttc acgctgcccg aacgtggcaa gcgtcgcatc cacaagacac
     3241 ccagccgcac cgaggtggtg gcttgtcgac cctggctgtt cgccgagatg atgtcggtgg
     3301 agcccgatgt ggtggtgctg ctcggtgcga cagccgcaaa gtcgttgatg ggcagcgact
     3361 ttcgcttgac cgcgcaccgt ggcgaggcat tgcacttacc gacgaccgac gacgtcacca
     3421 acctcgattt cgaccccgag atcgtggtgt ccgcacaccc ctcgtcggta ctgcgtgggc
     3481 cgccggagga ccgcgagaag gcgttccacg cgctggtgtc ggatctgcgg ttcgccgcca
     3541 agcggctgaa gcgtaagaga tgatcggtta ggtgcagtgc gtcgtcgatc gcgtcgtccg
     3601 gctgcccgac ggacggtcga tgggttatgc gcagtacggc agtgacggcg gctttccaat
     3661 cgtcaacgcg cacggtggtc tggcctgccg ccttgacgtc gccgccgcga acaccgtcgc
     3721 ggccgaagcc gacgtgcggc tgatctcccc cgatcgcccc ggtatcgggc tgtcggatcc
     3781 acagcctggc cgcacggttc tcgactgggc gaacgacgtc gcggacctct tcgaccgcat
     3841 cgacgtcgat cgattcgcag tgatgggctg gtcgatgggc ggacagtacg ccgcagcggt
     3901 gggtcatgcg atgccgcacc gggtcacgcg cctcgcgatc atcgcgggtg cgctgccgct
     3961 gaccgatccc ggggtgttcg acgaactgcc gaccatggac tgggttctga ccgggatgtc
     4021 tcgccgagcg ccgtggctcg cggcgcacag tggttccggt tcatgcggtt cgcagcaggt
     4081 gcctcgccca cactgtacgg acgattggcc gcgcgcgagc tggggccggc tgacggcgcg
     4141 gtcgtccgca atgaaggctt cgacgcgttc gcccggatgt ccagcgaagc cttacggcaa
     4201 ccgtccggcg ccgtcgaaga gtatcgggca tgggtgcggt cgtggggttt cgctcccgag
     4261 gacctcacgg tgccagtcga cgtctgggcg gggacccacg acgaattagt caacccgagc
     4321 tggtcgcagc gcctcgtttc gcgaatcccc aacgccacct tgaatattcg cgaaggggtc
     4381 acttcctggc gcacctgtac taccgggaga tcttcgcggc gcttacccgc tagtacaggt
     4441 cgacgcggcg gtcgtcgaat aagtggacgt gctcggtcaa tgtcttgtcg tgcgtggccg
     4501 ccagatcgat gtcggcgacg gccatggcga tgccgggacc ggatgcggcg acgacccacc
     4561 cgtcgggatc gatgatcgcg gtgccctcgg tccactgctg accacgctcg acgccggccc
     4621 ggtcacagca tgcgatcgcg accttgttgg tcctggctgt cgacatcgcg gtgatgacct
     4681 ctcccggatg ctcaccctcg ggccgcggaa acagcggcca gttgaccgga gcggcgatga
     4741 gttctgcctc gtccagggcg acccgtcggg tgagctcccc gaattcgagg tcataacaga
     4801 tcatcaccgc gatggcgcca tgcttggtct tgacgaccgg cggcagcgca tcgcccggcg
     4861 tgaagatcag cttctcgcga tcccacaggt gggtcttgcg atagctggcg acgacaccgt
     4921 cggcgtcgac catcaccgcg ctgttgtaga gccgtccgtc gtcgcccaat tcgcagaagc
     4981 caccgatgac gagcgcatca tcaacggatg cagaccaatt ggcgaagtct gtcggcctga
     5041 gcgcggtcgc ccgtgcttcg tcggcatcgg cgaacatgta gcccgatgtg gcgagctccg
     5101 gcagcacgat cacgtcggcg ccgacaccga ccgcctcacg gatctgcgcc tcgatcaccg
     5161 cggcattggc ggccagatcg ccgaccacag gctcgatctg gcagcacgct atctgcacca
     5221 tgatgggagg ctacgggacg cgtgcgctgc ccgcgtcctg gacattttgt ccaggacaga
     5281 gtcgttcctt ggacattttc gccatagcgt ctcgggccgc ggcggcgcat ggtgtgacgc
     5341 gtggaatccc ccgctgccgc caggatcgaa aagcgctcca ttgattatgt gccggagaac
     5401 gaacgccacg gcaaggtcag ccaccaaggg ccattctggt tcgtcgggaa ctttcagccg
     5461 ttcacgctgg cgctcggctt cgtcggcccc agcctcgggc tgtcgctgtg gtggagcatc
     5521 gtcgccggga tcgcggggat agcattcggc acgctgttca tggcgtttca cgccacccag
     5581 gggccggtgc tcggactgcc gcagatgatc cagtcgcgcg cccagttcgg attccgtggt
     5641 gtgctgcttc cgctgatcgg cacgctgttc acgtttgtcg gcttcaacgt cgtcgatgtc
     5701 gtgatcatca agtcggggct gcaatcgatc ttcgggtgga atccagttgt cgtagctgcc
     5761 gcgatcaccg tgatcgcggc gctgctcgcc atcttcggac acgacctgtt gcacaagtct
     5821 ttccgggtct tgttttggat ctccttgccg ctctggatca ttctgaccgt cagcgtgctg
     5881 ttcggcggtg tgacgggtgg tcccgccgca ccgggaggct tctcgtgggt cgggttcctc
     5941 gcgcagttcg gcgtcgcagc gtcctacaac atcacctatg cgccatacgt ttcggactac
     6001 agccggtacc tgccccgcga cacgaagccg actgcgatca tcgcgtcggt gttcgtcggc
     6061 gccgccggct cccccgcatg gttgatcccg ctcggcgcct ggatggccac atatcttggt
     6121 gccagcgacg cgctcgcggg gatcaacgag acggggaatt cggcgatctc gtacctgggc
     6181 agtgtgctgg cgatcgtgtc gactctcgtg ctggtggcca cgatgggctt gaacgcctac
     6241 agcggaatgc tcactgtggt aacggctttg gactctctgc gtccggtcaa accgacacgg
     6301 cggctgaggg tcgtgacgat cattgtgctg gcggtggcct ggttcgtcat gagcctgctg
     6361 ctgaccaatg cgacgacagc actgaacacg acgctgctga tcatgctcta tctgctggtg
     6421 ccgtggacgg cggtgaacct gaccgactac ttctttgtcc gtcgcggtca ctacgcgatc
     6481 gcggacctgt tcatacccaa cggaatctat ggcgcatggt cgtggcgggg gctcaccgct
     6541 ttcgtggtcg gcatcgccgc cgaaatcccg ttcgtggtgc tgccgttctt catcggaccc
     6601 gcggcagccg cgatgggcga cgtcgacatc gcgttcatcg tcggcctgct ggtgtcaggg
     6661 gtggtctacg tgctactgac gcggtcactc gacgtgtcgc atgagttgga gttgatcgag
     6721 tcgcatccgg aactggccga cccaatcagt tagtgcgtca gcacccccac gctgcgggcg
     6781 gcacccactg ccacacggcc ggcggcaccc agtacccggc accgcacggg gccggtggcg
     6841 cctgccagcc gccattccag ccgccgttcc agtcgccgtc gccccaatcc caatcaccac
     6901 cgtgaccgtg cccatggcca cgatcgagct tccacgtcat cccgggggta ctgggcagcg
     6961 gttgatcggc gctcgcgaca cccgcgccca tcccgatggc agtgaaagcc aatgccgaaa
     7021 cgaccgccgc tccggctccg atcctcttcg cattcacgat gaaatcccta ctactacagc
     7081 aaaatccgac taccaacgcg aaattcatcc tatccctatg attctgagct ggatatgcgc
     7141 tgtggacttc gaaccgacgt gaagcgcttt cagcaaaatt gacgcacgcg cgttcagcta
     7201 tggaatgaac gttgctgtcg gaccgcgaga ttggcgatta caagtttgca cggcaacact
     7261 gtgatcgcat tgtccggcaa tgtccgcaaa aattacctcg acaggtgtcg tcgatcacca
     7321 ctcggtgcga cgggaacggg cggcaccggc ggcaccctcc gattagcgga ggcgccgcaa
     7381 ccttcagctg tacagggcct cgaagcgctg gatcgaccgt tcgatgtcgc ccttgaccgc
     7441 ccgggccgcg gctgccccga ttggcccgaa cagcggtctg ccacccagat caagcctgaa
     7501 cgtgaacttg gcgccggagg ctgtcggaga aaccgtcatc cgcagcgcgt acttcgtgcc
     7561 gccaacgccg tcgccggtca tctcgagcat gcgcggcggc tccacctcgc gtaccgtcca
     7621 ggtgacccga ttgcgtaatc ccttggcgcc ggctacgccg acgaacgtgg ctcccgttgt
     7681 gagctcggcg ggcacctcgc cgcgccaccc ttggtgcatc accaaccagt cgccgaggtg
     7741 ggccagatcc gaggcatgtg cccaggcctc ctcgggagtc accgacaact cgcgggacag
     7801 ctcgagtttg gccacgcgca gacctttcag gtgatggacc gattcccgtg gttccagttg
     7861 ggtttctggt agacgagcca gcgcatctca atcggaattt cctcgcgggg gaggtcgaaa
     7921 acgtcgcgac cgctgaccca gtcggcgtac cagctcgtgg gtggccaggc cccttggggc
     7981 aggtgcgatt tctcgtagag gtacacggag tcgtccgaga ccagctgcag cggcaagccg
     8041 gacgccgccg ccgataactc ttgcgacgtg aaaatgcttg tatagcactg ttgtccgagt
     8101 tcgcgtgcgg cgtcgtcggg cgtatatccg ggtttggcga ggaaggcatt gaacaccagc
     8161 tgaccacccg cgaccaggca gtgggtggcg agtccgaaga cggcgcgcaa tcgctgggtg
     8221 gtccgaaagt cggacaccac ctcggaaagc aggatcagcc aatagtcccg ccgcaggtct
     8281 tcgtcgctgg agaagatgtc ccgctcgatc acgcgaacgt tgagtgactc ccgctcggcg
     8341 tcggtccgga tgatgtccgc gaacttcgga gtcatctcca ccacgtctac tggatggccg
     8401 cgtcgcgcta gagccagggc attgcggccc gttcccccac cgatgtcgag cacgcggtgt
     8461 gtcgccgcat cgggtgcctg ggtcgccaaa gcccatatgc gagcatccgg ttcggttccg
     8521 aacagtggtg gctcgcgcgt gccgatccag ttcgcgtacg cgccctcgac ggtccaccat
     8581 tcggccttga cgtgatagtt gagcactgtg ccgacgggag cgtcgaacga aatgacgatg
     8641 tttgaccgcg gcgagttcgc ataggcctcc gcgagctggc cctcgagcac ggacttgaca
     8701 tgggcgagtt gttccgcggt gaacggtctg cccactgttg caaacaggct ctcgcacatc
     8761 gacatgtact ggtcgatcat gcccggcact gcgggcaacg tgatctgtcc gttgactatc
     8821 gaccggcgat gcaaccgccg ggtcattgcg tcgcgctgcg ccgccgcatc gaccgggcgc
     8881 aagggtggac catccatcgc tgctagacgt tagggaaggc gcgcagccgc gcacggctat
     8941 tgcgtaatgt gtcgcgagcg agtggcggga ttcgaacccg cgtagacggc tttgcaggcc
     9001 ggtgcctagc cactcagcca cacccgcaac gggacatgac cctgccatcg tggccggcac
     9061 cggcccagcg tttgaatcgg cgtgatcgct actgctcgtc catcggcacg ccgactgcat
     9121 cagcgccctg cggccagccg gccggcgagt cttcccacgt ctcttggcgc ccatacggag
     9181 tcagatcgag gaagggatag ccgggcgccg agtgatccag cccgcgggcg aacgccgagt
     9241 acgtgtggaa cacgtcatcg ccgcgccgca ggaagacgct ggcacccggc cagtcgccgc
     9301 gcagtgcatc gtcgccgaac ccggcggcgt gcaactcgtc gagtgacttg tagttgtact
     9361 cgatcggcgt ccgcttcggg tccagcgtga cgtggtagtc gtagctgaag tcgttgtcgc
     9421 gcgacgagta ccacgggaag gtccaaccgt gctcggcgtt gtagcgcgcg atgctggcat
     9481 acggcgcccg cgacaccgcg gcgaacgtga cgcccttggc gttcaacagt gcgcgatcct
     9541 tctccgtgaa ggttaggtcc gctgccatcg tgcaactcgg gcaacctttg tcgatcgcgt
     9601 cgatccacat gaagtggtgg atgtacagct gcggacgccc ctcgaacatg tcgagcagcc
     9661 gcagctctcc gtcgggtccc tcgaacacgt agtccttttc gaccttcacc atcggcagcc
     9721 ggcgccgttc ggcgttgacg gcgtcgcgca gatgagtcac ctcgcgctcg cgagccagca
     9781 gcttcttgcg ggcggccagc cattcctcgc gagtggcgat ctcgggatag gcggtgggag
     9841 ttgtaggcat tatgtgtctc ctaacgaatc aggcctcgga aatatggact ccgcggcgcg
     9901 gcgaaagtca tcggttcatt cccggcgggt taatttgttg gcatgcaggt ctggctggcc
     9961 gacccgccgt tcttcggcgg ccctggacag ggcgcgcgac agatcgtcga gctgtttgtc
    10021 gccttcggcc tgacctcgtt gatcggcctg gagcgcgaga tccagggcaa gagcgcaggc
    10081 gtacgcacgc agaccatcgt cggcaccgcg gcggccctga tcctgctggt cagcaagtac
    10141 ggcttttctg atgtgctcca ggacggcctc gtcatcgtcg acccgtcgcg cgtggcggcc
    10201 caaatcgtct cgggcatcgg ctttctcggc gccggcatca tcatctttcg ccgcggatcg
    10261 gtgcacgggc tgaccaccgc ggcggcggtg tgggaatccg cggccatcgg tatggcggcc
    10321 ggggcaggca tgctgctgtt ggcggtcacg gtgaccgcga tgcacttcct catcatcgtt
    10381 ggcttcatgc ccctggcgaa gcggctgtcc gcacggctgg cgggatcggt gaccatgcac
    10441 atcacttatg aagagggccg cggcgtgatg agtcgtctgc ttcaggcgtg cgaccggcgt
    10501 cagtggcagc tcaccgacct cgcggcggac gccgcaggcg agcgcgtcag cccgggaagc
    10561 acaggagtaa tgctgacatt gtcgggcagg gggatcctga cggcgccgac cgtgctggcc
    10621 ggcatcgatg gcgtgacggc gatccgccaa ctcgacgacg aacccgactg accgggactg
    10681 gaattactag ccggaacggc aaagacacgg cagcgggaga gacagcgcgg gcccaagcca
    10741 gcgagtctga gcgccatgac atcgaatcag attgcggcac agtacgactt catcgtctgc
    10801 ggagccggtt cgtcgggttc cgtggtggcc cgccgtatgg ccgagaaccc cgaagtgggt
    10861 gtgctgttgt tggaggcagg cggcagcgac gacgtgccag aggtgctgga agccgcgcaa
    10921 tggcccgcca acattggtag cgagcgcgac tagggtttcg tcgcccaacc gaacccgcac
    10981 atcaacggcc ggtcactccc gctgtcgatg ggcaaggtac tgggcggcgg gtccagcatc
    11041 aacgtgatgg cctgggtccg cggacatcag aacgactggt gctacttcgc cgccgaggcc
    11101 ggcgacaaat catggaacta cgagtccgtg ctcgacatct accgccgaat ggaggactgg
    11161 cacggtgcac cggatccgaa gcgccgcggt gtgagcggtc tggcgcatgt cgaaccctcc
    11221 cccgatcccc attgcctcgc catggccgtg gtcaaaggtg ctgaatccgt ggggatgccg
    11281 tcctttgaca gcaacaacgg ccgaatgatg gaaggccacg ggggtgcgtc gattctggat
    11341 gttcgagtcc gtaatggtca gcggcagtcc gctttccgcg cgtacgttac gctctatctc
    11401 aagcggccga acctgacagt tctcacccac gcgctggtaa cccggctgtg catgcagggc
    11461 gaccgggtaa ccggcgtgga gtttcatcac aacggcaaga ttcgccgcgt cggagcgggg
    11521 tccgaggtca tcgtgtcact gggcgcgatc catactccga agctgctgat gcagtcaggc
    11581 attggcgatg cggacgaact gcgccggttc ggcatccccg taacacagca cctgccgggc
    11641 gtcggtcaga actttcagga ccacgtgctg gtcccctgca acggagtatc gggatccgat
    11701 ttcgccgccc agccccgaat cgtatgcgac tttcttctgg aagagcgaca tggcgctgga
    11761 cacgcccgtt ctccaagcgt gcgtcttgcc aacgccgtgg tcaacccccg aagttgcagc
    11821 gcgatttgat atcccggagt acgggtggac gatgtgtgcg ggcgtggtgc gcccggagag
    11881 ccgtggccgc gtccgcctga cagggcccaa cccacttgat cccgtgtgca tccagacgaa
    11941 gatgctcgcc gactcccgcg acatgaaggc ggccattgag tgcgtcgaat tgtgtcagga
    12001 gatcgccgat tcggcggcgt tgcggccttt cgtcaagcgc gcgacggcgc cgggcattct
    12061 gggcggcggt gacctcgagg actacatccg gggtaccgcc caaacatatt ggcatcagac
    12121 atgcaccgcg aaaatgggcc gggacgacca ctccgtcgtc gatgggaatc tcaaagtgta
    12181 tggaattgag cgtcttcgga tcgcggacgg ctcgatcatg ccgcgggtcg ccaccgggaa
    12241 caccatggcg ccctgcatgg tgatcggcga acgagcgggt gcggtcgtca aggcagacca
    12301 tggcctttaa tggctactgt gaccatcgga ctgatctgcg cgatccccca agaactcgcg
    12361 cacctgcgtg caaacctcgc cgattcccgc agcgaagagg cggcgcacac cgtcttcgat
    12421 accggcacga tcgacgggca tgacgtggtg ctggccggct cagggatggg caaagtcaac
    12481 gccgcgctcg tcaccacgct gctggccgac cgattcgagt gccgcacagt cgttttctcc
    12541 ggcgtagccg gtgggctgga tccgcgactg gagattggcg acatcgtggt tgccgatttc
    12601 gtcattcaac acgacgcggg tgtgctggag aacgaacgcg tgcggacgta ccagcctggc
    12661 tacgcaccgg tgatcaaccc cactgaccga ctcggctacc ccgtcgatcc cggacttctc
    12721 gaccgcgtca ggaagcggct cgaggggctc tcaataccag gccaaatcgt ctacggcaca
    12781 gtgttgtccg gcgatcagta cctcaactgc gagaccaccc gtgaacgatt gttgagggag
    12841 ctcaagggcc aggcgatcga gatggaaggc ggcgccgtcg cgcaggtctg cgaggcgttc
    12901 ggcatcccat ggctcgtcgt ccgtgccctg tccgacctcg cgggcggcaa cgcgctcttc
    12961 gatttcaccg ctttcgtcga tcaggccgcc gccgcttcgg ctacgatcct gcggcgcctg
    13021 ctgcctgtgc tctgaacgaa gccacagcgc cgaacgccgc cagcacggcg aacacggcga
    13081 acaaccaccg cgccggcgtc gcatctccgg tatccgtgag attcaccgcc acgcccgcca
    13141 gtcccgcgcc gaaggcaccg cagatcaatt ggacggtgtt gatcgccgcg gccgccgccg
    13201 gcccttcggc cggatcgtcg acgcaacaca tcgcccacgc cgatagatgc ggccacgcaa
    13261 taccgacccc ggccccggtg atcatcagcg ccgccgccca taccgcgacc aaccacaggg
    13321 ccgcatccgc gaactgggtc agcgcaccga gagccaaccc cgtggccatc accagcggtg
    13381 cgaccgccac cgtccgcctg atcacccgct cgctgctcag cgacgcgctg ctgatctcgc
    13441 cgacagtcca gccgatcgcg agccctgcac cgaggaatcc ggccgcgacg ggcgtcaggt
    13501 gggccagccg ctggccgaac agcggcacgt acatgtcgac catcgtggcg gccatcagca
    13561 cgccgagggt caaatagatc catttgagcg gcccggagca gaaggtgctc ggcggcagca
    13621 cggacgccgt cagccggcgg tcgacgtagg cgaacacggc gacgagtgcc gctccgagag
    13681 ccaacaacgc agtggtcgcc caaacgtcgt gcgggatccc ggccacactg acggcgagtg
    13741 cgacggcgcc aagcagcagc agtgaccaca cgggaatcgg tgtgcgcgtg tcttcgacgt
    13801 cggccgcgcg tcgcgcgggc agggcgatgg gtaccagaat cgccatcgcc gcggtcaaca
    13861 ccaccagaat cccgaacgcc caacgccacg agctgaattg ggcgaacagc ccgcccgcgg
    13921 ccggaccgac cagcgtgccg acaccccaca tcgccgacac cagagccgag gccttcgtcc
    13981 ataacgtgct tggcaaggcg gtgttgatca ccgcgtagcc cagccccgcg agcaggccgc
    14041 cggccgcgcc ctgcacggtg cgcccgacca acatgatctc catggtcggc gcggccgcac
    14101 atgtcaggct gcccacgccg aacacgctga gacccaacag gtatgaccac cgcgggccaa
    14161 gccgcatcag tagcgagtgg acggtcgtcg ccgctacgac agaggcaacc aggtagaccg
    14221 tggtcaccca cgcgtagaac cgctgaccgc cgatgtctgc caccgcgctg ggcatcaggc
    14281 tgatggtgag gaattcgttc gtcgcataca gcgcgacacc gccggcgagc acggtggagg
    14341 ccccgaggtt cttcgggcct agcagctcac gccagctacc ccggatgatc gtcgcggtgt
    14401 cggtcacgcc gctcacgtta ggagctcaac cgctgttgag gtcaagtctt ataacactgt
    14461 cgatgaaatg ccgtaccgcc gggcgatcgt tggtcgtcga caccaccatt gcgatacgcg
    14521 cttcgggatg cgcgtcggcc agttcccggt acacgacgcc gtcgagcccg atgctgttga
    14581 tcgaccgcgg caccagcgcg acaccgagtc cggaggcgac caggccgacc accgtcggca
    14641 tcgcggtgca ctcgtacgcc accgctggcg tgaatcccac ccgggtgcag gcacgggtca
    14701 gctcgtcgtg gaaatcgggc gccgaatcgc ggggaaacag cacgaacggt tgatcccgta
    14761 agtccctgag ccgcaatcgt tctcgtcggg acaagtcgct tgtggccggc agcacagcga
    14821 cgacgggctc gtgccacacc ggatacacga ccagtccggc cgtcgtcgcc ggtggccgaa
    14881 cgaatgcgac atcgagccgc tggtcgtgca ccgcacggat gtgctcgccg ctcgacccct
    14941 cgacaagtcg gagcgcgacg tcaggcgccg ttgcccgcca cggcggaacg atccgcggca
    15001 ggatggccag cgccgcggag ccgaggaatc cgacgcgcag caatcccgcg cggccggcgg
    15061 ccgcggtgcg ggcggcattc tccgcaaggt cggcctgcgc caaggcctcg cgggcgtgcc
    15121 tgagcatcgc gtggcccgcc tccgtcagct cgaccttgcg acggttgcgt tcgaacagcg
    15181 tcgcgccgac ctgccgttcg agtcgctgaa tctgctggct cagcggcgac tgagcgatgt
    15241 gcagccgctc ggctgcgcga ccgaagtgaa gctcctcggc cacggccacg aaggaccgca
    15301 ggtgccgcaa ctccataaga cgtttaccat atcaccgcct acctactttg tctggaaacc
    15361 ggcatagaga tcggtaaggc tggagccatg aacccgagaa acgaagccga cgtcattgtg
    15421 gtcggggccg gatccgcggg agcggtggtg gcgtctcggc tcacggaaaa tcctggccgg
    15481 acggtgctgt tgatcgaagc cgggccggat ttcgggaccg gcgtcgagac ccagccgcag
    15541 gacatcatcg acgcggagga ctcgaccgcc accgcattcg attggcagca caccggcacg
    15601 ccgtcgggcc tgcaccgcga aatacccctc tacgcaggca aggtcgtcgg cggcagctcg
    15661 gccaccaata acgtcatggc attgcgcggc gaccccgcgg tgtacgacag ctgggcgatc
    15721 gacggatggt cattcaagga cctgctgccc gccttctgcc ggctcgagcg cgatatggat
    15781 tacagcgaca gggcgtggca cggcgatgcc ggtccggtca ggatccgccg tcacgtcgcc
    15841 ggccccgtcc acgcagcgtt tctgaacgtc gctgcgacac aaggacatcc gtcggtcgat
    15901 gaccacaacc gaccgtcggc agttggtgct ggtgcactgc cgctgaacca gatcaacgga
    15961 gttcgccagt cgaccacact gacatacctc agggttgcca ggacacggcc gaatctgtgg
    16021 gttcgcgcga acgcgaccgt ggaccgcgtc ctactcgacc atggttcaac ccgcggtgtt
    16081 cgcctggcca atggcgcaga gatccacgct gattcggtga tcctcagtgc tggcgcattg
    16141 ggcagcccgg cgatcctgtt gcgctccggt atcggcccgg ccgaagacat acgcacactc
    16201 ggtattccgg tggttgcgga tcggcggggt gtcggcgaga acctgcacga ccacccgctg
    16261 ctgcgcatga cgttcagcac tgacggggaa cgcgcacggc cagtgcgaca aacggtgctc
    16321 accgctcgca ccggcaccga cgccgcgcct gatttgcagc tatttcccag cgggccgaca
    16381 ccggccgacg acggtgccgt gctcaccatg ctggtcgcgc tgttgacacc gcggtcgcgc
    16441 gggcgcatgc ggttgacggc ggcagatccg ttggcgccgt tggacatcga cccgggtcat
    16501 ctcgaggatc gcgcggatct gccccgcgtc gtcgctggcg tcgaactggc ccgagagctc
    16561 ctcacgagcc cgcccctcgc gcgatacatc caggcggccg cgccagatac ccggtcgctg
    16621 atgtcggagc agggtgtgaa cttgcgatcc gcgatcgtcg accagctgaa cacgtaccac
    16681 catccggtcg gcgcctgccg gatgggcccg cccgacgacg acatggcggt ggtcgacccg
    16741 gccggaagcg tctatggggt cacgggtttg tcgattgtgg atgcgtcgat cttcccgacc
    16801 atccccagcg cgaacacgaa tctcccgacg ctgatggcgg ccgagcacct agcaccggca
    16861 ctgactactt gaggcccgcg gcgtccatgc cgcgcagttc ctttttcagg tcatggatct
    16921 cgtcacggat gcgcgccgcc aactcgaact gcaaatccct tgcggcggcc atcatctgct
    16981 cggtcagatc cttgatgagg tcggcgagtt cggcgcgcgg catgttcgtg gtgtcgcgcc
    17041 cctcgatgat gcccgcgctg acggcccggc ccggctcccc ctgggcgcgg cggccacgcg
    17101 aggcgttgcg gcccgagccg ccgacctcga cgtcgtcggc ctcgcggtac acctggtcga
    17161 ggatgtcggc gatcttcttg cgcaacggtt tcgggtcgat gccgttggcc tcgttgtacg
    17221 cgatttgttt cgcccggcgc cggtcggtct cgtcgatcgc ctcccgcatc gagtcggtga
    17281 tgttgtcggc gtacatgtga acctcgccgg acacgttgcg ggcggcgcgg ccgatggtct
    17341 ggatcaggct gcgcaccgac cgcaggaagc cctccttgtc ggcgtcgagg atcgccacca
    17401 gcgacacctc cggcaggtcg agaccctccc ggagcaggtt gataccgaca agcacgtcgt
    17461 agtctccgag tcgcagctgg cgtagcagtt cgacgcgacg caacgtgtcg acctccgagt
    17521 gcaggtagcg aacccggatg cccatctcca gcagatagtc ggtgaggtcc tcggccatct
    17581 tcttggtcag cgtggtgacg agcacccgct catcgcgttc ggtgcgcttg cgaatctcac
    17641 cgatcaggtc gtcgatctgg cccttcgtcg gcttcacgat gacctgcggg tcgaccaggc
    17701 ccgtcgggcg gatgacctgc tcgacgaact caccgccggt ctggctcagc tcatagggtc
    17761 cgggcgtcgc tgacagatac accgtctgcc cgatccgatc ggcgaactcc tcccaggtca
    17821 gcggcctgtt gtcgatcgcc gacggcaacc ggaagccgaa gtcgacgagg ttgcgcttgc
    17881 gagagatgtc gccctcgtac atcccgccga tctgcggcac cgtgacgtgc gattcgtcga
    17941 tgacgagcag gaaatcctcg ggaaagtagt cgagcagcgt cgccggagcc gaacccgcag
    18001 gccgagcgtc gatgtggcgc gagtagttct cgatacccga gcagaacccg acctggcgca
    18061 tcatctcgac gtcgtagttg gtgcgcatcc gcagccgctg cgcctccagc agcttgccct
    18121 ggccctccag ttcggacagc cgctcctcga gttcggcctc aatggtcgag atcgcgtgtg
    18181 ccatccgctc cggtccggcc acgtaatgcg tggccgggaa aacgcgcagg gaatcgacct
    18241 tgcgcaccac gtcgccggtc agcgggtgca tgtagtacag cgactcgatc tcgtcgccga
    18301 agaattcgat gcgcacagcc agttcctcgt acgacgggat gatctcgacg gtgtcgccgc
    18361 ggacgcggaa cgcgccacgg gtgaacgcca ggtcgttgcg gttgtactgg acgtcgacca
    18421 gcagccgcag cagcgcatcc cgtggcacct cctggccgac ctgcagctcg accgagcggt
    18481 ccaggtacga ctgcggtgtg ccgaggccgt agatgcagga caccgacgcg accacaacga
    18541 cgtcgcggcg ggacagcagg ctcgacgtcg ccgagtgccg cagccgctct acgtcgtcgt
    18601 tgatcgagct gtccttctcg atgtaagtgt cggtctgcgc gatgtacgcc tcgggctggt
    18661 agtagtcgta gtacgagacg aagtactcga cggcgttgtg cggcaacatc tctcgcagtt
    18721 cattggccag ctgtgcggcc agcgtcttgt tcggcgccat caccagggtg ggccgctgca
    18781 gccgctcgat cagccacgcg gtggtcgccg acttaccggt gccggtcgcg ccgagcaaca
    18841 ccacgtcgcg ctctcccgcc ttgatgcggc gctcgagttc ctcgatggcg gtcggctggt
    18901 caccggccgg cgcgtgcggg ctgaccacct cgaagcggcc gccgacccgg acgatctcgt
    18961 cgacggcgcg gtactccgag tgcgctagca cggggtgttc ggtagcgaaa gccatgttca
    19021 ccagggtaga acccggcacc gacatgcgtg attcccgcgg ctaggccccg atgaagcggg
    19081 cgggacgacg ttcgatgaac gacagcatgc cctcggcccc gtccttcgtc cggaacaagg
    19141 ccacgacgtc gtcgacaaac cggccctcgg cggcctgctc cccctcgcgg ttcgcgcgct
    19201 gggcggcggc gagcgtcgtg cgcaccgcca gcggcgccga ccgcgtagcg atccgttctg
    19261 ccagttcaac ggcgcggtcg agctggtcgc ccggtgccac gacctcctgc accagcccga
    19321 ttcgcagggc ctcgggggcg tcgaattcgt cgccggtcaa cagccagcgc atggcgttgc
    19381 cccagcccgc ctcgcgcggc agccgcgtgg tcgccccacc gaagggatag atgccgcgct
    19441 ggacctcgag ctgcgcgaag cgcgcgtccg cgctggcgat gcgaacgtcg gcggccagta
    19501 agagctcgat gccgagcgtc aaacaccagc cctgaaccgc cgcgacgaca ggctttgtcc
    19561 acggtcgtcc gtcgttgcgc cagggattgc gggcgtcgtc cggccagtcc agtgaccctt
    19621 ccatcaggcg tggtccgact tcggcgagat cgagcccggc ggtgaagtgg tcgccattgg
    19681 cgtacagcac gccaacgcgt aggtcgtcgt cgcgttccag gcgctcgtat gcgcggccga
    19741 gctcgtccag catttcgacg ttgaaggcgt ttcgttttgc gggtctgttg agtccgatcc
    19801 gaagaacgtg gtcctgttgg tcgactatga cacttggcat agttgcgagc ctaaactatg
    19861 atgaccgtca taaccagcga tagccggaaa cggatgatcc aaagcgccgc tttgctgttc
    19921 agggagcatg gctacagcgg cacgggcttt cgcgacgtca tcgagcacag cggcgcgcca
    19981 cgcggttcga tctaccacca cttccctggc ggcaaggaac agcttgcggc cgacacggtg
    20041 gcgtgggccg ccgcggtgat cgagcgacgg atagaacgcg ccgcgcgggc cggcaacccg
    20101 ctcgtcgcgc tcagcatgtt tattgacgcg tggcgagagg tgctagagga cagcaacttt
    20161 cgcgccggct gcccgatcgt tgctgtcgcg gccgaggccg acgcgggatc gaccgccatc
    20221 gcagcggcgg ccgtcgcgtt cggccgctgg caggacctca tcgcacacgc gctgctccgc
    20281 gcgggcgttg gccgcacgga tgcccggcga ctggccaccc tcgtgattgc gtccatcgaa
    20341 ggcgcgatcc tgctctgccg cgcccgccgc gacatccggc cgctgcgcga cgtccatcgc
    20401 gcgctcgagg cgacgctgcg tactgccatt tcatggcacg gccgaccggt ggagtgatcc
    20461 aaccggcggc ctatgctgag atatccacgc tcccaagcac gaaacattcg acctggtcgc
    20521 ccgcaccaac accgatccga agggcatcgt gcgggccgtc gacgtctaca ccgtcgagcc
    20581 gtggggcctc tacatggcgc ggccgacgcc gggccgggcg cagtttcact acatggagtc
    20641 atggctgttg ccctctttga gcctgcgcgc cacggtgttc cacttcaacc ccggctacga
    20701 actcgaccag gactattacc tcgacgtcgg cctgtacacc gcgggcgcga ccgcatggca
    20761 tgccgaagac cactatctgg atcttgtcgt ccgaaccgac gtcggcgtgg agttgtcgga
    20821 cgtcgacgag ctgctgacgg ccgtgcggca cggcctgctc accccagaaa cgggtgaaca
    20881 cgcggtgcaa accgcggtga gcgcgatcga ggggctgtcc aggcacgact atgacctcaa
    20941 tcgctggctt gccgacaacg gcatggcgct gacctggcgc ggggcgtagc gcgtatccgg
    21001 ggcgggtatg ccccggttat gaccttgaag gagtaccgca acaagcggcg gtcgggccgc
    21061 agccctgagc cacgcggacg tcgtcgccgg gcccgcaagc agccccgctt cgtcatccag
    21121 caccatgccg ccagcaccga ccactacgac ttccgtctgg agatcgaccg tgtgcttgtg
    21181 tcgtgggcgg tgccgaaagg gccgtcgacc aatccgaagg acaagcggat cgcgcgccgc
    21241 accgaggacc acccgatgga gtacgaaacg ttcgagggca tcattccgaa gggcgagtac
    21301 ggcgccggcc gcgtcatcgt ctgggaccgc ggcacgtaca ctaccgagcg cgacatggcc
    21361 gaggcgctgg agcgcggaca tctgtcgttc cagctggacg gcgagaagct ccacggcggc
    21421 tacgcgctca cccggctgcg ccagggtaag gacgaggcat ggctgctggt caagaaggcc
    21481 gacgatgacg ccgacgcgcg acgcaaaccc gtacgcagcc aaccggaatc ggtgctgtcg
    21541 ggccgcaccc tcgacgagtt gtcgtgaagg ttccccagtg gcgggcaccg accctggcca
    21601 cgttgaccaa tgagcgcttc tccgatccgg agtggatctt cgaacgcaaa ctggacgggg
    21661 tgcgcgggct ggcgttccgc gacggcaagc atgtgcgact gatgtcccga aaccgactgt
    21721 cgctcaacgg aacctaccct gagatcgttg aggcacttac gagccaggac accccacggt
    21781 tcgtcgccga cggcgagatc gtggcattcg agggccggcg taccagcttc ggccggttgc
    21841 agggccgctc gggcatcacc gatccggacc aggcccgcgc ctccggcata cctgtcttct
    21901 actacgtgtt tgatctgctg cacctcgacg gcaacgacac tacccagctg ccgctgatcg
    21961 agcggaaacg gctgctgcgc aacgagttcc agttcgccga tccgctgcgc tacaccaccc
    22021 accgggtcaa ggacggcgag gccgcctacc gcgcagcatg cgaacgcggc gacgaaggtg
    22081 tgatcgccaa acatgcggat tccccgtatg agggcgggcg ctcgacgaac tggctgaagt
    22141 tcaagtgcag ccgcgaccag gaattcgtgg tcgtcggcta cacgagcccc aagggcagcc
    22201 ggatcgggct cggcgcactg ctgctcggct accacgacga cggcgatctg gtctacgcgg
    22261 gcaaggtcgg cactggcttc gacaccgcga cccttcgcgg cctgcacgag cggttgtcgc
    22321 ccaccgacca gaaggcgcca ccgatcacca agggccttgc ccgcgagccg ggtgcccatt
    22381 gggtgcgtcc gacgttggtg gcgcaggtgg cgttcagcga gtggacccgc gacggcaagc
    22441 tgcgccaccc gcgatatgtc ggtttgcgca ccgacaagag ccccgacgac gtggtgaggg
    22501 agacgcgctg atgccggtca aggtggagat cacccatccg gaccgagtgc tgttccccga
    22561 cgacggcatc accaagggcg atgtggtcga ctactacgcc gaggtggccg cggtgatggt
    22621 gccgcatctc aaagggaggc cgctgacgct gtggcggtat ccccgcggaa ttgatcagca
    22681 cggcttcgtc cagcaggact tcggcggcgc aatgccggac tggatgggtg cggccgaggt
    22741 cgccaaggaa ggcgggaccg tcgtgcatcc cgttgccgac cgccgcgacg ccctggtgtg
    22801 gctggccaac cagaactgcg tcaccccgca cgcctggctg tcgcggcgtg accgcctcga
    22861 cgtacccgac ctgatcgtca tcgacctcga tcccaccacg accgacttcg cgcctgtgcg
    22921 ggcggcggcc cgcgcctgtg cggacgtcct ggacgatctc ggtttgacgt cgtatctgaa
    22981 gacgacgggg tcgcgcggcc tgcacgtcgt ggtgccaatc agccgtggcc ccgactacga
    23041 cgcggttcga cagttcgcgc gcgatgtcgc cgac
//
LOCUS       DASRJA010000137         3342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_737500,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000137 DASRJA010000000
VERSION     DASRJA010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737500"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="VFC01_06290"
     CDS             complement(<1..1265)
                     /locus_tag="VFC01_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79283.1"
                     /translation="MLAGLPADFVENRGQWDGPARFVAWKGATVASFESDAIRLRVGK
                     DRSSSMVLAFEGAVSKDARLIGENRRSGYYNFFLGNDATRWRSNVPAYSGVLYREIYD
                     GVDVRVREEGGRLEYDVLLLPGANLEQVVIRADGASRLALESDGSLTLDTPTGSVRQT
                     PPRTSEVLESGETRAKASRFRIIDAQRFGFEVPDHNSRLALVIDPGLEWSTFLGGSNR
                     EEIHGLALARDGSGDVVVAGHTWSSDFPTAPAGSLGASPLIPFVARLNSTGTALVYAT
                     LFGGTNGNVSYAFGLTLDATSAPVVVGETNAANFPTTPGAFQPTFNEPSATINRGWDA
                     FVTRFNSSGSAMVFSTFLGAAPIFDPSLPGSQRGGDESARVVVMDATDSVIVSGYTTS
                     ENFPTTAGAYDRTHSTLTVPVQGGTISSR"
     gene            complement(1447..2709)
                     /locus_tag="VFC01_06295"
     CDS             complement(1447..2709)
                     /locus_tag="VFC01_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79284.1"
                     /translation="MTVSTSATITALMGGVSRTATLTVNPAPPPAPSAPSLLSPANQA
                     TVSQPITFDWTDAANATTYNIQIDNSSNFTTPLTLSQTVGVSQATITGLPAQQLWWRV
                     RGINSAGVAGPFSPSRRFTAQTAPATASLSALSVSPSSVVGGAAAQGTVTLTSGAPSG
                     GAVVSLSSSSGSASVPASVTVAAGATTASFTVSTSTVAASTSATLTATYNTVSRTATL
                     TVTPPPPAASLSALSVSPTSVTGGNTAQGTVTLTSGAPSGGAVVSLSSSSNSASVPAS
                     VLVAAGATTVSFTVSTSTVAASTSATLTATYNTVSRTVVLTVTPVPTGPLPAPTLSSP
                     AADARFLPGQNIVFDWSDVTGATSYTIQIDDQDTFPAPQIVSQTVTPSTYSNSTLPTR
                     QMWWRVRANDSAGNPGTWSSVRRVEVKN"
     gene            2687..2971
                     /locus_tag="VFC01_06300"
     CDS             2687..2971
                     /locus_tag="VFC01_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79285.1"
                     /translation="MAEVETVTEDVVTVNVRLVAPEATVTLAGTLTALELSDSDTTAP
                     PAGAAALKVTVPVEELPPATLVGLSDSVERVTPGDPDGVSVSVALCVTFS"
     gene            3056..3331
                     /locus_tag="VFC01_06305"
     CDS             3056..3331
                     /locus_tag="VFC01_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79286.1"
                     /translation="MTVVGTTARRVLLLARSTTAFPVGAAARKVTVPVEGLPLETLPG
                     FKATEETALETTRNSVSLTVVPPDDAVMTTGVVAVTAAVLTVKVALV"
BASE COUNT          517 a         1103 c         1169 g          553 t
ORIGIN      
        1 atccgcgagc tgatcgttcc accctgcacg gggacagtca gcgtcgaatg cgtccgatcg
       61 tacgctccgg ccgtggtcgg aaaattctcg gatgtcgtgt agccggagac gatgacggag
      121 tcggtggcat ccatgacgac cacccgggcg ctctcgtccc cgcctctctg cgagccgggg
      181 aggctcgggt cgaagatcgg agccgcgccc aggaacgtcg agaacaccat cgcgctgccc
      241 gaggaattga atcgcgtgac gaaggcatcc catcctcgat tgatcgtcgc ggagggctcg
      301 ttgaatgtcg gctgaaacgc gccaggagtc gtcgggaagt tggcggcgtt ggtttcgccg
      361 acgacgacag gggcgctcgt ggcatcgagg gtcaggccga aggcatacga aacattaccg
      421 ttggtgccgc cgaatagcgt cgcgtaaacg agggcggtcc cggtggaatt gagccgcgcc
      481 acgaagggga tcaacggact cgcgccgaga ctgcctgccg gcgcggtcgg gaaatcgctg
      541 gaccacgtgt gtcccgcgac caccacgtcg cccgaaccat cgcgtgccag cgccagcccg
      601 tggatctctt cgcggttcga gcctccgagg aaggtggacc actccagccc cgggtcgatc
      661 acgagggcga gccgagaatt gtggtcggga acctcgaagc cgaagcgctg tgcgtcgatg
      721 atccggaacc gggacgcttt ggcccgcgtt tcaccggact cgagcacctc cgaggtcctc
      781 ggtggcgtct gccggaccga tccggtcggg gtatccagcg tcaacgagcc gtcgctttcc
      841 agcgcgagcc gagatgcccc gtccgcgcgt atgacgacct gctcgagatt ggctcccggg
      901 aggagcagca catcgtattc gagccgtccc ccttcctcgc gcacgcgcac atcgacgccg
      961 tcgtagatct cacggtacag cacgccgctg taggccggga cattcgatcg ccaccgcgtg
     1021 gcgtcgttcc cgaggaagaa gttgtagtag ccgctccgcc tgttttcgcc gatgagccgt
     1081 gcgtccttgc tcacggcgcc ttcgaaggcc aggaccatgg agctcgatcg atccttaccg
     1141 acgcggagtc ggatggcgtc gctctcgaag ctcgcgaccg tggctccctt ccacgccacg
     1201 aacctggccg gtccatccca ctggccgcgg ttctcgacga agtccgccgg gagcccggcc
     1261 agcatcgagg atgcagaatc gagagggagc cggccgaaac ggagcgtggc agatgtcgcg
     1321 gtgggggggc ccgcgctcgc ggcctggctg aacagggtga ccgccaagag gccgaagacg
     1381 agcagaatca gagaagggtg tctcacgttc atgaggacct cccgatcgga cggaatgcga
     1441 cgcgccttag tttttcacct cgacacgtcg gacggacgac caggtgcccg ggttgcccgc
     1501 cgagtcgttg gctcgcacgc gccaccacat ctggcgcgtc ggcagcgtgc tgttggaata
     1561 cgtcgacggc gtcaccgtct ggctcacgat ctgcggcgcc gggaaggtgt cctggtcgtc
     1621 gatctgaatc gtgtagctcg ttgcacccgt cacgtcgctc cagtcgaaca cgatgttctg
     1681 acccggcagg aagcgggcgt cggcggccgg gctgctgagc gtcggcgcgg gcagcggacc
     1741 cgtgggaacc ggcgtgaccg tcagcaccac cgtgcggctc accgtgttgt aggtcgccgt
     1801 gagcgtcgcg ctcgtgctgg cggcgacagt gctcgtgctg acggtgaagc tcaccgtcgt
     1861 cgctcccgcc gcgaccagga cgctcgccgg cacgctggcc gagttcgagc tgctgctcag
     1921 gctcaccacg gcgcccccgc tcggcgcccc gctcgtcagc gtcaccgtgc cttgcgccgt
     1981 gttgccgccg gtcacgctcg tcgggctcac gctcagtgcc gacaggctcg ccgcaggagg
     2041 cggcggcgtg acggtgagcg tcgcggtccg gctcaccgtg ttgtaggtcg ccgtgagcgt
     2101 cgcgctcgtg ctggcggcga cagtgctcgt gctgacggtg aagctcgccg tcgtcgctcc
     2161 cgcggcgacc gtgacgctcg ccggcacgct ggccgagccc gagctgctgc tcaggctcac
     2221 cacagcgccc ccgctcggtg ccccgctcgt cagtgtcacc gtgccttgcg ccgcggcacc
     2281 ccccaccacg gagctcgggc tcacgctcag ggccgacagg ctcgccgtgg ccggcgccgt
     2341 ctgggccgtg aaccggcgcg acggcgagaa cggacccgcc accccggccg agttgatgcc
     2401 ccgcacgcgc caccagagct gctgcgccgg cagcccggtg atcgtcgcct gggacacccc
     2461 gaccgtctgg ctcaacgtca gcggcgtggt gaagttgctg gagttgtcga tctggatgtt
     2521 ataggtcgtt gcgttcgccg catcggtcca gtcgaacgtg atcggctgcg agacggtggc
     2581 ttgatttgcc ggactcagca ggctcggcgc cgacggggcc ggcggcggcg cgggattcac
     2641 cgtcaatgtg gccgttcgcg acacccctcc catcaaggcc gtgatcgtgg ccgaggtgga
     2701 gaccgtcacc gaagatgtcg tgacggtgaa cgtgcggctg gtcgcccctg aggccaccgt
     2761 gacactggcc ggcacgctca cggcgctcga attgtcggac agcgacacga cggctcctcc
     2821 ggcaggggcc gcggcgctca aggtcaccgt tccggtcgag gagttgcctc ctgccacgct
     2881 ggtcgggttg agcgatagcg tggagagagt gactcccggg gatcccgacg gagtgagcgt
     2941 cagcgtggcg ctttgcgtga cattctcgta ggtggcggaa atcaccacgg tcgccgttgt
     3001 ggagactgga gcggtattga ccgtgaacgt ggcgctggtc tgacccgctg gcactgtgac
     3061 ggtggtcggc accacggcca ggagagtgtt gctgctggcg agatcgacga ccgcattccc
     3121 cgtcggcgcg gccgcgcgca aggtcacggt tccggtcgag gggttgccgt tggagacgct
     3181 gccggggttc aaggccaccg aggagaccgc cctggagacg accagaaaca gcgtctcgct
     3241 gacggtggtg ccgcccgacg acgcggtgat gacgaccggc gtggttgccg tgacggcagc
     3301 ggtgttgacc gtgaaggtcg cactggtctg acccgccggc ac
//
LOCUS       DASRJA010000138        22527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4121066,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000138 DASRJA010000000
VERSION     DASRJA010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22527
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4121066"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VFC01_06310"
     CDS             <1..300
                     /locus_tag="VFC01_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HZN79287.1"
                     /translation="EWYDELVRLTDSPIVRLNRAVAVGEADGPQAGLAALAELDPTLP
                     RYTASDAYLHERAGDIDTATKLYVEAAAQAQNLAERNHLTLKAATLRQRLSGEGG"
     gene            complement(312..1010)
                     /locus_tag="VFC01_06315"
     CDS             complement(312..1010)
                     /locus_tag="VFC01_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79288.1"
                     /translation="MRTYGQYCPIARGAEIFAERWTPLIIRNLYLGCGSFNQILEGAP
                     GLSRTLLSQRLKHLERLGIVESATKPGGHGRHYELTPAGQDLFTVCKSLGEWGAHWLE
                     IAPEHLDPFVALWSMCNMLRRDRLPERRVVIRFDFTGGPRTERYWLLIEHGDPEICKT
                     CPGLDEDLYITADAEAFVKWHAGQQTWAEATRDGRIQLHGPTWLVRGFPTWNARSGFA
                     HIKPVSRPAASLAG"
     gene            1117..2487
                     /locus_tag="VFC01_06320"
     CDS             1117..2487
                     /locus_tag="VFC01_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN79289.1"
                     /translation="MEINGFRGQLITPDDADYDTARAVWNGAVDRRPRLIARCGGTAD
                     AAAAVRFARDHDLEIAIRGGGHNVAGSAVCDDGIVIDLSAMRAVCVDPVARTALVQGG
                     ALWGDVDHETQTYGLATTGGIVGHTGVAGLTLGGGLGFLMRKHGLTIDNLLAAEVVAA
                     DGSIIRASADEDPDLFWGLRGGGGNFGVVTTFRFALHPVGPTVMAGPIFWAADDTVAV
                     LRFYRDFTAEASDELGTVVRLGTIPPLPVIPEDLHWRPAIAVACCYVGAVEDGERAVR
                     ALRGLGAPLVDLLAPTQYAGFQGALDDTVLHGWHYYWKSTNLAGLSDYTIDVVADHAY
                     AATSPRSYSAMFHMGGAVARVPDDATAYGSRDVAHNIIIDAVWLPEESGNHASTETAW
                     ARAFLNALQPHRTDSVYVNFLDSDDDACRLKEAYGDHTYQRLAEVKAKYDPDNAFHHN
                     RNIRPA"
     gene            2600..3523
                     /locus_tag="VFC01_06325"
     CDS             2600..3523
                     /locus_tag="VFC01_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN79290.1"
                     /translation="MAHIDSVGRSPRLPMPRLPKVPALAPVGQIPDGAVVDLPGRGST
                     YVFDSGPSDGPTYVLLHSLACTGLMTWYPALDMVREFGRVVIFDQRCHGQGISPPRML
                     LEDCADDVAALADVLDISSFVPVGYSMGSLIAQLVWKRHRDRVDGLVLCAAAAAVSRA
                     RYERVATGMFAALIETLSPAPRRCEPAAGVMGGLFRDHQWVLGQLRATSPGGITRAIA
                     EVVRFDSTAWASEIDVPTAVVVTMKDRAFGVQRQKWLANRIPEAEMVTVDAGHSGCTL
                     QPAAFVPGLRQAIESVQRRLPFSRYGKRTAK"
     gene            3561..4976
                     /locus_tag="VFC01_06330"
     CDS             3561..4976
                     /locus_tag="VFC01_06330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZN79291.1"
                     /translation="MKRLSGWDVLLLSSETPNVHQHTLKVAVVDTEGFQGEPTFEAFR
                     EVFRSRLPVLDPLRFQLVGTPWRLHRPMWRENAEVNLDYHLRRVPVPAPGGRRELDAV
                     VGAVASTPLDRRYPMWQLHFAEGLAERRIAVIGKVHHVLADGVASANLMARAMEWPGS
                     QPLEQEPLPSAPPPATAELLKFAVRDHVTRLRTLPSAIRDGVVGAYRLQRRSRQRHRH
                     PDLADRFDPLPTFLNHKLSPARTFASATLPLADVKAISKKLDVTINDVVLTIAAGGLR
                     RLLLNYGESADGPLIASVPTATNVSPDRITGNALATMLVSLPVQIDDPMERLRLIRTS
                     TRIAKEDHELLGPTLVGQWLEFVPPALTRMVFRWTSRREAPNQLFNVIVSNVPGPRER
                     GRIAGAVVTEIYSVGPLAAGSALNVTVWSYVDQMSISVLSDDCTFRDTHDVTEALVAA
                     FTELREGAAAAGTTDIPPATP"
     gene            5101..5574
                     /locus_tag="VFC01_06335"
     CDS             5101..5574
                     /locus_tag="VFC01_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN79292.1"
                     /translation="MEARLNMIENPVGTKIWKYIVSAGKAVADSPLPATTQELVKIRA
                     SQINGCSGCLDMHTKEAAHAGETSTRLNLIAAWREAKVFTDAERAALELTEQGTRIAD
                     AAGGVTDDAWANAVKHYDEDQLAALVSLIALINAFNRGNVIIQQPAGDYEVGQFG"
     gene            5712..6692
                     /locus_tag="VFC01_06340"
     CDS             5712..6692
                     /locus_tag="VFC01_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZN79293.1"
                     /translation="MQLGFGFWASKTLLSAVELKVFGTLSEAGPLNADELGERLGLHP
                     RGARDFFDALVALRMLDRTGGRYSNTPETDLFLDPAKPTYVGGVLEMSNARLYTFWGS
                     LTEGLRTGQPQSEVKTGGDFFETLYADPEKLAQFTGAMTGLSRAAGDAIAAKFRWRDY
                     GSVIDIGCAQGAVPAAIATAHNHLSGGGFDLPPVEPIFNSYVTQFGLADRLRFTPGNF
                     FDDPLPSADVLIMGHILHDWELDQKRLLLQKAYDALPTGGALIVYEAIIDDDRRHNAF
                     GLLMSLNMLIEGLGFDFTGPDCRGWMADVGFSESYVEHLVGPDSMVVGIK"
     gene            6723..7031
                     /locus_tag="VFC01_06345"
     CDS             6723..7031
                     /locus_tag="VFC01_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79294.1"
                     /translation="MILATDGTAYAWAIGLDQGGTGIAWWSPGSGVVRVIAKGRPSDT
                     SFQTGTTVVDTRSGAATYLRELVAHADGGTIALNLRGPGKSESIAGVVRVDALPPLSC
                     "
     gene            7132..7887
                     /locus_tag="VFC01_06350"
     CDS             7132..7887
                     /locus_tag="VFC01_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN79295.1"
                     /translation="MKIVVIGGTGLIGSKLVHTLTEHGHDAVAAAPSTGVNTFTGEGL
                     PEVLAGASVVVDVSNSPTLDDAAIEFFRTATTNLLTAEKDAGVGHHVALSVVGTDQLA
                     PQSGYFQGKLLQEKLIGGGPIPYSIVHATQFFEFLKTLADSATVGDTVRMPPAYFQPM
                     AAADVAEGLAIAAVNAPVNGITEIGGPEAFLLPDLIRTALTARGDAREVVADPKAQYW
                     GIDIAERTLVPGEGARLFDTRFEDWILEAAAKA"
     gene            8074..8739
                     /locus_tag="VFC01_06355"
     CDS             8074..8739
                     /locus_tag="VFC01_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HZN79296.1"
                     /translation="MDVYGAVTSRRAVRAFTDQPVPHEVLQRVLCAAAWSPSGSNLQP
                     WHIYVVTGAPLAQLKKLAVERVAAGDPWDESEYVMYPPALKSPYAERRAAFGKERYSA
                     LGVAREDWEARQRAAIANWDCFGAPAALFCYIDRDLGLPQWADLGMYLQSVMLLLRAE
                     GLHSCPQMSWAKVRKTVAEFVTPPDELVLFCGMSIGYEDVTVSHPRTGRASLDETVTF
                     VEG"
     gene            8803..11595
                     /locus_tag="VFC01_06360"
     CDS             8803..11595
                     /locus_tag="VFC01_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN79297.1"
                     /translation="MSSEGRGPRLRGRDSECETLRGLLSTAQSGSSQVLVLRGEAGVG
                     KTALLDYTAELASAFRCVHVVGVQSDMELAFAGLQQLCAPLLNHLDELPEPQREALNV
                     AFGRGVGAAPDRFLVGLAVLSLIAAAANDRPLFCIIDDAQWLDQVSVQTLAFVARRLL
                     AEPVALVFAARDAGAEALAGLPKLVIRGLSDGDARELLESVMVGGIDPRVRDRIVAET
                     RGIPLALLEVPRSVSATELAGGFWISGKRSSAAAIEDDFIRRIRSLPEPTQRLLLVAA
                     AEPVGDAALFLRAAAQLGIPVDSLAPAEAAGVIEFGPRMRFHHPLMRSAAYRAADLTE
                     RRAIHRALADATDPQSDPDRRAWHAANAAAGPDDAVATELEVSADRAQSRGGIAAAAA
                     FLERATALTSDSTQRGARALAAAQAKRDAADPAAAHELLAIAELGPLSDLQRAQVARL
                     RAQMEFARSRGGDAHSPRLLEPASQLLHAAKRLDTLDDDLAREAYLEALAAAMYAGRL
                     GEPGATATVAEAASAVVDRAPNLHRPIDFLLAAMAKRITGGASAGSAPLRTALELMRS
                     QAEQNDGQALRWMSLGLAIVEESAVGELWDDAIYHQLATDTVQQARDAGALAVLPPAL
                     GYRAGVHVLAGEFATAATLIEEAASITAATDYAPLRYHSLTLAAWRGTSADAVGLIEA
                     AAADGAARGEGRVLGLTGYLSAVLYNGLGRYEEAFVAARDACEYEELGFHGWCLFELI
                     EAATRTGDRETAAHAAQRLEERTSPSGTDWGLGALANARALLSDDDVADGLYTEAIER
                     LARTRIVVHLARTHLHYGEWLRRMNRRVDARRHLTEAHEMFTRMGAQAFAERARRELV
                     ATGEKVRKQAVSSGNELTAQEAQIARLAGDGLTNQEIGAQLFISTHTVEWHLRKVFVK
                     LGITSRRQLRTVSWAS"
     gene            11726..12298
                     /locus_tag="VFC01_06365"
     CDS             11726..12298
                     /locus_tag="VFC01_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN79298.1"
                     /translation="MTLTSVSTPSKSDQDLAAQFAREAEPLFEVLSRGARRLARSDAD
                     AEDLLQDTLLHAYAGFRTFREGSNLKAWLFRILYNRWVSAYRSKQRRLSEVSVDGITE
                     RDLVDSAARIPAGLRSAEAEVLDALPDSDIKAAMEALPDGFREAVYYADVQGYTYAET
                     AAILGVPHGTVMSRVSRGRRRLRIALADVA"
     gene            12391..12822
                     /locus_tag="VFC01_06370"
     CDS             12391..12822
                     /locus_tag="VFC01_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN79299.1"
                     /translation="MSKDYDAEWENALTVVQEAQPPFIPEDAHAMTVVIDYPPGSAGA
                     PPHRHPAGPAFGYMLEGEMLFELEGQPPRVVRAGEAFWEPGGDVIHYSDANNRDDMRC
                     RFTVTMLCVSGQPMLTLVDDEELAARKHLRVPAEPNMSKPL"
     gene            12837..13703
                     /locus_tag="VFC01_06375"
     CDS             12837..13703
                     /locus_tag="VFC01_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79300.1"
                     /translation="MSRILLQTTITESADDWDISRFSLLADELRAAGHDVTARNRVSS
                     DGSDRVLSRLDEMEYDQLWLMAVDLGDGLTAADAYSVRRFRENGGGVLTARDHQDLGC
                     CLNRLGSLGVVNEFHDKSVDPSTMCDDQDTPTISWPNYHSGANGDYQPVLIEGPVHEL
                     LRTNRTPSRRIEWFPAHPHEGMVSANGPFATAIAQGRSTATGRHFNLAVVLDGELTPD
                     GRPMGRAVAESTFHHFADYNWDLDYGAPSFVSEPQGNQIKADPFRLEIFKDYVRNIAA
                     WLQPANSLSVAV"
     gene            14113..14304
                     /locus_tag="VFC01_06380"
     CDS             14113..14304
                     /locus_tag="VFC01_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79301.1"
                     /translation="MADVKATAEEVVWAGANLAFGLPGFAPAFDKSTVGSVDPHHHCD
                     QSSKKRQTDKKNQSGCHDV"
     gene            14443..15069
                     /locus_tag="VFC01_06385"
     CDS             14443..15069
                     /locus_tag="VFC01_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN79302.1"
                     /translation="MAIDSRGQPLGEKTVQATSAGNASALKCARLKFGPELTWAIEDV
                     RTVSGRLEQELMSAGQSVVRVPTKLMAHTRASARTRGKSDSIDATEIARAALREPDLP
                     GAQHDPVSRDFRLLTERRDVLVEQRTATTNRVQWRIHELDPLRSRNLKPLIFAKNREP
                     VAAWLAELPGLVAELGCDELSASRCGEHVFRINRVFDNPFLQRIDFLR"
     gene            15089..15394
                     /locus_tag="VFC01_06390"
                     /pseudo
     CDS             15089..15394
                     /locus_tag="VFC01_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900212.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(15509..17029)
                     /locus_tag="VFC01_06395"
     CDS             complement(15509..17029)
                     /locus_tag="VFC01_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HZN79303.1"
                     /translation="MTDRDDALRRQPGWTGPEESDHDPETGPPGPAEPSPSADSALPR
                     RPPMDFVPPEPQERAGEPTRADEPRPFSTGPHRLRAEPPPLPPQPEGSRLGPAEGPPL
                     FVDPDRSRGSFREPPQVGPHPQPPAPGALQNAPPAASRVETGEAGGQLGSQVPRAPQP
                     AQPPPVPGGWTGQRFPGPRHGPDTGAQPAPPASYAEGIRVDELWPARRVPPIRGWRLA
                     LYKATFGLVHVGPSADEIRLAELEARIKSVLRGHYKVGVMGKGGVGKTTVSACVGSVF
                     AELRYDDRVVAIDADTAFGKLGSRVDPKAPGSYWELASDQYLESFADVRARVGNNAAG
                     LFVLVGEATPARRRVLDPAIYREATSRLDRYFTISIIDCSSTMDSPVTQEVLRDLDAL
                     IVVSSPWIDGAAAAGQTMDWLASRGLIGLLRRTVIVLNDSDGHADKRTRSILAQQFGS
                     HGQAVIEVPFDGHLRPGGVIDRTTELLPATRRRFVEIAATLAEYFPTQDDRSRERN"
     gene            17454..18101
                     /locus_tag="VFC01_06400"
     CDS             17454..18101
                     /locus_tag="VFC01_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79304.1"
                     /translation="MGLLLVRLVELLIVIVPVIGLVVAGMKAVSAARERQAHGGDESV
                     PQANTQAAQWRAISRTVKEHDRTDTRWLDYELDIGKLLDFPLMTDMRNPLTERFHRAK
                     LRADLLRPAKAEDLLEDGDAAREYLDAVENYVTAFDVAESEAIRRRRNDFTKPEQQRL
                     TRARNALRVAVDAGATLQERERAYALASRELDGLIVLPERARAAIERGILGELDG"
     gene            complement(18102..18794)
                     /locus_tag="VFC01_06405"
     CDS             complement(18102..18794)
                     /locus_tag="VFC01_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN79305.1"
                     /translation="MDRLFGAAVVATSALLGTAINIPTASAEPCPDVEVIFARGTFEA
                     PGVGGTGQAFVDALRGQLGDKSMDVYPVNYPASLDFATAADGVVDASNKIRDVAASCP
                     STKMVLGGYSQGAAVVAYTTEDSVPNGFTLPAGLTGPMAPEVAKHVAAVVLFGKPSSG
                     FLQMIYTGAPPITVGHLYADKTTDLCIPEDNVCSPTGNDNGAHNLYSVNGMAVQAADF
                     AARRVLSNGRAA"
     gene            complement(18924..19685)
                     /locus_tag="VFC01_06410"
     CDS             complement(18924..19685)
                     /locus_tag="VFC01_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018162352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN79306.1"
                     /translation="MAERVTFGSSTGPSLAGIIDRPAGEPRGWGVFSHGFTLGKDSPA
                     AARICKQLAEDGIGMLRFDALGLGDSEGDWGDGSFTVKVNDVVRACEFMAEQGTPADI
                     LVGHSFGGAAVIAAARQAPGVRAVATVGAPVDPSHAEQHYDAVVDRVLSEGSAEWMAG
                     GRTLTLKRAFVEDVRAAALHDKIRGLRMPLLILHSPTDNTVGIENASEIFRTARHPRS
                     FVSLEGSEHLLTGPGQARRAARIIGAWADAYLSTE"
     gene            19740..20657
                     /locus_tag="VFC01_06415"
     CDS             19740..20657
                     /locus_tag="VFC01_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZN79307.1"
                     /translation="MSEQVTTHAPDPADVGKRIDPVLARSWLLVNGAHADRFQAAAHS
                     RADIVVLDIEDAVAPKDKAAARDNVVRWLEAGNSDWVRVNGFGTPWWADDLKALASTS
                     IGGVMLAMVESVDHVTETAKRLPNVPLVALVETARGLERITEIAAAKGTFRLAFGIGD
                     FRRDTGFGEDPATLAYARSRFTIAAKAAHLPSAIDGPTIGSNPLKLIEATAVSVEFGM
                     TGKICLTPDQCVVVNEGLSPSQDEIAWAREFFAEFERDGGEIRNGSDLPRIARATKIL
                     ELARSYGIQVSEFDDEPVHMPAPSDTYHY"
     gene            complement(20756..21154)
                     /locus_tag="VFC01_06420"
     CDS             complement(20756..21154)
                     /locus_tag="VFC01_06420"
                     /inference="COORDINATES: protein motif:HMM:NF018933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZN79308.1"
                     /translation="MMRRKADSAGGNRRYEYNPDTTALSQTCLCGNRKKKTLSQRVHR
                     CECGVLEHRDLFSAYLGLHVHRRTDGLDRMDLGAANHGWLLRRQDVDGSPRSDRKASI
                     NRRGRRHPPSRRSVARIKARRAAKRQSRSA"
     gene            complement(21303..>22527)
                     /locus_tag="VFC01_06425"
     CDS             complement(21303..>22527)
                     /locus_tag="VFC01_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HZN79309.1"
                     /translation="AGHQFGGFVPRLGDGRALLLGEIACQDGTVRDIHLKGSGVTPFA
                     RGGDGLAVVGPMLREYVISEAMHALDIPTTRSLAVVATGRRVHRDTLLPGAVLARVAS
                     SHVRVGSFQYAATAGDADLVRRLADHAIARHHPNAAEAENPYRALFEAVVAAQASLIA
                     QWMLVGFVHGVMNTDNMTISGETIDYGPCAFMEAYDPATVFSSIDSWGRYAYGQQPAV
                     AAWNLARFAETLLPLFADDLDDALALAQESLDGFRRHYVDASMAGMRAKLGLRDVDDA
                     VATPLIAELLEQLEQSRIDHTSFYRLLGRAARGDVEPARGLFVDLAAFDGWLARWRAL
                     IPDADAMDRVNPVYIPRNHLVEEALAAATDGDVDPLTQLLAAVTAPYDERPGFDRYAE
                     PAPEDFGAYRTFCGT"
BASE COUNT         3934 a         7505 c         7342 g         3746 t
ORIGIN      
        1 gagtggtatg acgagctcgt ccggctcacc gacagcccca ttgtgaggct caatcgcgcc
       61 gtcgccgtcg gtgaggcaga cggaccgcag gcggggcttg ccgcgctcgc cgaactcgac
      121 ccgacgctcc cgcggtacac cgcttcggac gcctaccttc acgagcgggc cggcgatatc
      181 gacacggcga caaagcttta cgtggaggca gccgcacagg cgcagaacct agccgagcgt
      241 aaccacctca cgctgaaagc ggcgaccctg cgccagcgcc tctcgggtga aggtggctga
      301 acgccaagac gtcagcctgc caggctcgcc gcaggtcggg agactggctt gatgtgggcg
      361 aaaccactgc gggcgttcca agtgggaaat cctcgtacga gccacgtggg gccgtggagt
      421 tggatgcgcc cgtcgcgggt ggcttcggcc caggtttgtt ggcctgcatg ccatttcaca
      481 aaagcttcgg cgtccgcggt gatgtagaga tcctcgtcaa ggccggggca agtcttgcag
      541 atctcggggt ctccgtgctc gatcaacaac cagtaacgct cggtgcgtgg gccgccggtg
      601 aagtcgaagc ggatgacgac gcgtcgctcg gggagacggt ctcgacggag catgttgcac
      661 attgaccaca gagccacaaa cggatcgagg tgctcgggcg cgatctcaag ccagtgcgca
      721 ccccactcgc caagcgactt acataccgtg aacaggtctt gacccgcggg tgtcagctcg
      781 taatgccgtc cgtgcccacc tggcttggtt gccgactcga cgatgccgag ccgttccaga
      841 tgcttgagcc gctgcgagag cagcgtgcgg gatagcccgg gtgcgccctc aaggatctga
      901 ttgaagcttc cgcagcccag gtacagattg cggatgatca gcggcgtcca gcgctcggcg
      961 aagatctcgg cgccgcgtgc aatgggacag tactggccgt aagtccgcac gacaccagtg
     1021 tcacaaacct gctctcggcg gggaagtcca gattcttgac ttcacggccg cggtctacag
     1081 cggtgtgatg agcctcgata cgaggaggca accgcgatgg agatcaacgg attccggggc
     1141 caattaatca cgcctgacga cgccgactac gacactgccc gggccgtatg gaacggtgcc
     1201 gtggaccgcc gcccccgact gatcgcccga tgcggcggga ccgccgatgc ggccgcggcc
     1261 gtgcgattcg cccgcgacca cgacctggag atcgccatcc ggggcggggg ccacaacgtg
     1321 gccggaagcg cagtgtgcga cgacggaatc gtcatcgacc tctcggcgat gcgggccgta
     1381 tgcgtcgacc ctgtcgcccg cacggccctg gtgcagggtg gtgcgctgtg gggcgacgtc
     1441 gaccacgaga cgcagaccta cggtctggcc accaccggcg gcatcgtcgg ccacaccggt
     1501 gtcgccgggc tgaccctcgg cggtggcctg ggctttctga tgcgcaagca cgggctcacc
     1561 atcgacaacc tgctggccgc cgaggtggtc gccgccgatg gcagcatcat ccgggcgtcc
     1621 gcggacgaag accccgacct gttctggggg ctgcgaggcg gcggcggcaa cttcggcgtc
     1681 gtcaccacgt tccgtttcgc gttgcacccc gtcggcccga ccgtgatggc tggaccgatc
     1741 ttctgggcgg ccgatgacac cgtcgccgtg ctgcgcttct accgggactt caccgccgaa
     1801 gcgtccgacg agctcggcac cgtcgtgagg ctcggcacca tccctccgct gccggtcatc
     1861 ccggaggacc tgcactggcg tcccgccatc gccgtcgcct gctgctacgt cggcgccgtc
     1921 gaagacggcg agcgggcggt gcgggccctc cgcgggcttg gggcaccgct cgtggacctg
     1981 ctggcgccca cccaatacgc gggcttccag ggcgccctcg acgacaccgt tctccacggg
     2041 tggcactact actggaagtc cacgaacctt gccggcctct ccgactacac catcgacgtc
     2101 gtcgccgatc acgcgtatgc cgccacctcg ccgcggtcgt actcagcgat gttccacatg
     2161 ggtggcgccg tcgcccgcgt gcccgacgac gcgaccgcct acgggagtcg cgacgtggca
     2221 cacaacatca tcatcgacgc ggtgtggctc cccgaagagt ccgggaacca cgcttccacc
     2281 gaaactgctt gggcccgagc atttctcaat gctcttcagc cacatcgcac agacagtgtc
     2341 tacgtcaact tcctcgattc cgacgatgac gcctgccgcc tcaaggaggc gtacggagat
     2401 cacacctacc agcggctcgc cgaggtcaag gcgaagtacg accccgacaa cgccttccat
     2461 cacaacagga acatccgtcc cgcttgagca ccgttgcgcg tcggttttga gccgcgggcg
     2521 tgcggaaaca ctgatggcat gaaccggtcc attcgtggtt tcgccgtgcg tgaccgacgt
     2581 tcttctggga cgtgttctaa tggcccacat cgatagcgtc gggcggtctc cccgccttcc
     2641 gatgccgcgg ttaccgaagg tccccgcgct ggccccagtc ggccagattc cggacggcgc
     2701 agttgtcgac cttcccgggc gaggaagcac ctacgtattc gattcgggac cctccgacgg
     2761 gccgacctac gtcctgttgc actcgctggc gtgcaccgga ctgatgacgt ggtatcccgc
     2821 gctggacatg gtgcgtgagt tcggcagagt ggtcatcttc gaccagcgat gccacgggca
     2881 aggcatctcc ccaccgcgca tgctgctcga ggactgtgcc gacgatgtag ccgcattggc
     2941 cgacgtcctc gacatctcga gcttcgtgcc agtcggctat tccatgggat cgctcatcgc
     3001 tcagctggtt tggaagcgcc accgcgatcg ggtcgacggg ctcgtgttgt gcgccgcggc
     3061 ggccgcagtc agtcgcgccc gctatgaacg cgtggccacg ggaatgtttg cggcccttat
     3121 cgagaccctc agcccggcgc cgcgtcgctg tgagcctgca gccggagtga tgggcgggct
     3181 ctttcgcgac caccaatggg tcctaggcca actccgcgcg acatcgccgg gaggcatcac
     3241 gcgcgcgatc gccgaagttg tccggttcga ttcgacggca tgggccagcg agatcgacgt
     3301 gcccacagcg gttgtggtga cgatgaagga tcgggcattt ggtgttcagc ggcagaagtg
     3361 gctggcgaat cgaataccgg aagcggagat ggtgacagtc gatgccggcc atagcgggtg
     3421 caccctacag ccggccgcgt tcgtccccgg tctgcggcaa gccattgaat cggtacaacg
     3481 ccgacttccg ttctcgcgct atggaaaacg caccgctaag taactaaacc tatgccaccg
     3541 gttctaggga ggagcggccg gtgaaacgac tcagtggttg ggatgtactg ctgttgtcca
     3601 gcgagactcc caacgtccac caacacaccc tgaaggtcgc ggtcgtcgac accgaggggt
     3661 tccagggcga gccgacattc gaggcgttcc gcgaggtgtt ccgcagcaga ctcccggtcc
     3721 tcgatccgtt gcggttccag ctcgtgggca ctccgtggcg gctgcatcgt ccgatgtggc
     3781 gcgaaaacgc cgaggtgaat ctcgattacc acctgcggcg ggttcccgtg ccggccccgg
     3841 gtggccgcag agaactcgac gccgtagtcg gtgcggtcgc cagcacacca ctggatcgcc
     3901 gatacccgat gtggcagttg cattttgccg agggtctcgc cgagcgacgc atcgcggtga
     3961 tcggaaaggt gcatcacgtc ctggcagacg gtgtcgcgtc agcgaatctc atggcgcgtg
     4021 caatggagtg gcctggctcg caaccgctcg agcaggaacc tctgccgtcc gcgcccccgc
     4081 cagcgacagc cgaactcctc aagttcgcgg tacgcgacca cgtaacgcgg ctgcgcacgt
     4141 tgccgtctgc gatccgcgat ggagtggttg gtgcctaccg actgcagcgg cgttcgcggc
     4201 aacgccatcg gcaccctgac ctggccgacc ggttcgatcc gctgccgacc ttcctcaatc
     4261 acaagctctc accagcgcgg actttcgcca gcgctaccct gccgctcgcc gatgtcaaag
     4321 cgatcagcaa gaagctcgac gtcaccatca acgacgtggt gctgacgatt gccgcagggg
     4381 gtctgcgccg gctgttgttg aactacggtg aatccgccga cgggccgctc atcgcctcgg
     4441 tgccgaccgc caccaacgtt tcaccggacc ggatcaccgg caacgccttg gccacgatgc
     4501 tggtatcgct gcctgtccag atcgacgacc cgatggagcg cctccggttg atccggacct
     4561 caactcggat tgcaaaagag gatcacgagt tgctcggtcc gaccctggtg ggtcaatggt
     4621 tggagttcgt cccaccggca ctgacccgca tggtgttccg gtggacgtcg cggcgtgagg
     4681 cgcccaacca actgttcaac gtgatcgtgt cgaacgtccc ggggccgcgg gaacgcggtc
     4741 gcatagccgg tgcagtcgtg accgaaatct actcagtcgg acctcttgcc gccggttccg
     4801 cgctgaacgt caccgtctgg agctacgtcg accaaatgag catctcggtg ctatccgacg
     4861 actgcacatt ccgcgacacc cacgacgtga ccgaagccct cgttgccgcg ttcaccgagc
     4921 tacgcgaagg agcagccgcc gccgggacga ccgacatccc cccggcgacg ccgtaggtgc
     4981 gctcacctcc ggccggaccg cagcttggac agtgacgggg gtcacattcg tggctgtcac
     5041 atctcgctga gatccggtgt cccaagggtg tacgcactaa caacgccaca ggagttcacg
     5101 atggaagctc ggctgaacat gatcgaaaac ccggtcggaa ccaagatctg gaagtacatc
     5161 gtctcggcag gtaaggcggt tgcggactcg ccgctaccgg ccaccacgca agaactggtg
     5221 aagatccgcg ccagccaaat caacggctgc agtggctgcc tcgacatgca caccaaagaa
     5281 gccgcgcacg ccggggagac ctcaacacgg ctcaacctga tcgcggcgtg gcgcgaggcc
     5341 aaggtgttca ccgacgccga gcgcgctgcc cttgagctga cggagcaggg cacccgcatc
     5401 gccgatgcgg ccggcggcgt caccgatgac gcctgggcga atgccgtcaa gcactatgac
     5461 gaggatcagc tcgccgcgtt ggtgtcgctc atcgcgctca tcaacgcctt caaccgcggg
     5521 aacgtcatca tccagcaacc ggcaggcgac tacgaggtcg gccagttcgg atagcgcatt
     5581 tcaccgcgat ttgatcgcgg caccgatagg ttcacgccgt agtcgggctt tgcggcgggg
     5641 ggatttcaga tgagcggacc gaccacgccg tcgacggccg agcaaacggc ggccttgaca
     5701 cccgcgggat catgcaactg gggttcgggt tctgggcgtc gaagacgctg ctgagcgctg
     5761 tcgaattgaa agtcttcggc acgctcagcg aggccggacc attgaacgcc gacgaactcg
     5821 gcgagcgact cggcctgcat ccgcgcggcg cgcgagattt cttcgacgcc ctggtcgcgc
     5881 tccgcatgct cgaccgcacc gggggccgct actcaaacac tccggagacc gacctcttcc
     5941 tggatcccgc gaagcccaca tatgtgggcg gagtactgga gatgtcgaac gctcgcctgt
     6001 acacgttctg ggggtcgctc accgagggtc tgcgaacggg ccagccgcag agcgaggtga
     6061 agacgggagg cgacttcttc gagacgctgt acgccgaccc cgaaaaactc gcccagttca
     6121 ccggcgcgat gaccgggctc agccgcgcgg cgggcgacgc gatcgccgcg aagttccgct
     6181 ggcgcgacta cggcagcgtg atcgacatcg ggtgcgcgca aggagcggtc ccggccgcga
     6241 tcgcgacggc ccacaaccat ttgtccggcg gtggtttcga cctgccgccc gttgaaccca
     6301 tcttcaactc ctatgtcacc cagttcggcc ttgccgaccg cctccggttc acgccgggca
     6361 acttcttcga cgacccgctg cccagcgccg acgtgctcat catgggccac atcctgcacg
     6421 actgggagct ggaccagaag cgcctcctcc tgcagaaggc atacgatgcg ttgcccaccg
     6481 gcggcgcatt gatcgtctac gaagcgatca tcgatgacga ccggcgccac aacgcatttg
     6541 gtctcctgat gagtctcaac atgctgatcg aaggactcgg gttcgacttc accggaccag
     6601 actgtcgagg ctggatggcc gacgtcggtt tcagcgagag ctatgtcgag catctggtcg
     6661 gccccgactc gatggtggtc gggatcaagt aggcccaggc cctccgccgc ggtcaagacc
     6721 aggtgatcct cgccaccgac ggcactgcct acgcatgggc gatcgggctg gatcagggcg
     6781 gcaccggtat cgcatggtgg tcgccgggat ccggtgtcgt gcgggtcatc gccaaaggtc
     6841 gtcctagcga cacgtcgttc cagaccggca cgaccgtcgt cgacacccgt tcgggtgcgg
     6901 cgacgtacct gcgcgagtta gtcgcgcatg cggacggcgg cacgatcgcg ttgaacttgc
     6961 ggggcccggg caagtccgag tcgatcgcgg gtgtggtccg cgtcgacgcg ctgcccccgc
     7021 tttcctgctg aaactgcagc tcctcaaaaa agttttggcg gcgggatgag ttcgcgatcg
     7081 gccagccgtt ctggcggtca ggtaagtcat agacacaacg aaaggcgcat catgaaaatc
     7141 gtcgtaatcg gtggcaccgg cctgatcggc tcaaagttgg tgcataccct gaccgaacac
     7201 ggtcacgacg ccgtcgccgc ggccccgtcg accggtgtga acacattcac gggcgagggc
     7261 ctgccagagg ttctcgcggg tgcctcggtg gtagtcgacg tctcgaattc gccgacgctc
     7321 gacgacgcgg cgatcgagtt cttccgcacg gcgaccacga atctgctcac ggccgagaag
     7381 gatgccggag ttggccacca tgtcgccctt tcagtggtgg gcaccgatca gctggccccg
     7441 cagagcgggt atttccaggg gaagctcctg caggagaagc tgatcggcgg cggtcccatc
     7501 ccttactcca ttgtgcatgc gacgcagttc ttcgagttcc tcaagaccct tgccgactcc
     7561 gcgaccgtcg gcgacacggt gcggatgcca ccggcctact tccagccgat ggccgctgcc
     7621 gacgtcgcgg aagggctcgc catcgccgcg gtgaatgctc cggtgaacgg catcaccgaa
     7681 atcggtgggc cagaggcgtt tctgcttccc gacctgatca ggaccgcgct gaccgctcgc
     7741 ggggatgccc gcgaggtcgt cgctgatccc aaagcacagt actggggcat cgacatcgca
     7801 gagcgcaccc tggttccggg cgagggcgcg aggctgttcg atactcggtt cgaggactgg
     7861 atcctcgaag ctgccgccaa agcgtagttg aaaacacctg ggcacgaagc taattcactg
     7921 agcgggaccc tgtgtcacgg tccggtccag tcgcgagata agagcgggag caccgggttt
     7981 caggcggcct tcgggtacgc gccaagcaga gagcgtggct ggtcccacct gccaccggac
     8041 cgatatccgg gcatgaaatg aggagaaacg atcttggacg tctacggggc ggtcacgagc
     8101 cgccgggcgg tgcgcgcatt caccgaccag cccgtcccgc acgaggtgtt gcagcgggtg
     8161 ctgtgtgcgg ccgcgtggtc accgtcgggg tcgaaccttc aaccgtggca catctacgtg
     8221 gtcaccggcg cgccgctggc gcagctcaag aagctcgccg tcgaacgcgt ggccgccggc
     8281 gacccgtggg acgagtcgga gtacgtgatg tacccgcctg cactgaaatc cccgtatgcg
     8341 gagcggcgtg ccgcgttcgg caaggagcga tacagcgcac tcggcgttgc gcgcgaggat
     8401 tgggaggccc ggcagcgggc cgccatcgcg aactgggact gctttggcgc gcccgccgcg
     8461 ctgttctgct acatcgaccg cgacttgggc ctgccccagt gggccgatct cgggatgtat
     8521 ctgcagtccg tcatgctgct gctgcgcgcc gaagggctgc acagctgccc gcagatgtcg
     8581 tgggcgaagg ttcgcaagac cgtcgcggaa ttcgtaacgc cgcccgacga gctcgtcctc
     8641 ttctgtggca tgtcgatcgg gtacgaggac gtcacggtga gccacccccg caccggcagg
     8701 gcttcgctcg acgaaacggt cacgttcgtc gaggggtagg acgaaaacga tgtgcgaccg
     8761 gtcgaccggt agggttcact gcaaacagac gcggtggagc ctatgtcgag cgagggtcgg
     8821 gggccacgtc ttcgcggtcg cgacagcgaa tgcgagacgc ttcggggtct gttatcgacc
     8881 gcacaatccg gcagcagcca agtactggtg ctccgtggcg aggcgggcgt aggcaagacc
     8941 gcgttgctcg actacaccgc agagctggcc tcagcgtttc gttgcgttca cgtcgtcggc
     9001 gtgcagtccg acatggagct cgcattcgcc ggcctgcaac agctctgcgc gccgctgctg
     9061 aatcaccttg acgagctacc ggaaccacag cgcgaggcgc tgaacgtcgc attcggccgc
     9121 ggagtcggcg cggccccgga ccggtttctg gtcggcttgg cggtgctgag cctgatcgcc
     9181 gccgctgcca acgaccgacc cttgttctgc atcatcgatg acgcgcagtg gctcgaccag
     9241 gtgtcggtgc agacacttgc cttcgtcgcg cggcgtctgt tggccgagcc ggttgcattg
     9301 gtttttgcgg cgcgcgatgc gggtgcagag gcgctagccg ggctgccgaa actggtcatc
     9361 agaggcctgt cggatggcga tgctcgggaa ttgctcgagt cagtgatggt gggcggcatc
     9421 gacccgcggg tacgggaccg catcgtcgcg gagacgcgtg gcattccgct ggccctgctg
     9481 gaggtgccgc gaagcgtctc cgcgaccgag ctggccggcg ggttttggat ttcgggcaaa
     9541 cgctcgtcgg ccgcagcgat cgaggacgac ttcatccgcc gcattcggtc cctccccgag
     9601 ccgacccagc gcctgctact tgtcgccgcg gccgagccgg ttggcgatgc cgcgttgttt
     9661 ctgcgcgccg ctgcgcaatt ggggattccg gtcgactccc tggcaccggc cgaggctgcg
     9721 ggagtgatcg agttcggtcc gcgcatgcgc tttcaccatc cactgatgcg ttcggccgct
     9781 taccgcgcgg ccgatctgac cgaacgcagg gccatccatc gggcgttggc cgacgcaacc
     9841 gatccgcagt cggaccccga ccgtcgggca tggcatgctg cgaatgctgc cgcgggaccc
     9901 gatgatgcgg tggccacgga gctggaagtc tcggccgacc gagcccagag cagaggcggc
     9961 atcgccgctg cggcggcatt ccttgagcgc gcaaccgcgt tgacatcgga ctccacccaa
    10021 cgcggcgcca gggcgctcgc ggcggctcag gccaaacgag atgcggcgga ccccgcagcg
    10081 gcgcacgaac tcctcgcgat cgcggaactt gggccactgt ccgacctcca acgcgcccag
    10141 gtcgctcggt tacgagccca aatggagttc gcccgtagcc gcggcggtga tgctcactcg
    10201 ccccgacttc tcgagcccgc gtcgcaattg cttcacgccg ccaagcgact cgacacgctc
    10261 gacgacgacc tagccaggga ggcgtatctg gaagcactcg ccgcggccat gtatgcgggg
    10321 cgccttggtg agccgggcgc aaccgcgacg gtggcggagg ctgcaagcgc agtggtcgac
    10381 cgtgcaccga atcttcatcg gccaatcgac ttcctgttgg ccgccatggc gaagcggatc
    10441 accggcggtg cgagtgccgg ttccgctccg ctgcgcaccg cgttggagct gatgcgcagt
    10501 caagcggaac agaatgacgg ccaggctctg cggtggatgt cgttgggcct ggccatcgtg
    10561 gaggaatctg ccgtcggcga actgtgggac gacgcgatct accaccaact ggccaccgat
    10621 accgtccagc aggctcgtga cgccggtgca cttgctgtac tcccaccagc actcggctac
    10681 cgggccggcg tccacgtgct cgcgggcgag tttgccacgg cggcaacact tatcgaagag
    10741 gcagcctcga tcaccgcagc gacagactat gcaccactga gataccactc gctgactctg
    10801 gctgcctggc gggggacttc ggccgacgcc gttgggttga tcgaggcagc cgccgcagac
    10861 ggagctgcga ggggtgaagg acgcgtgctc ggtctgactg ggtatctcag cgcggttctc
    10921 tacaacgggc tcggccgata cgaggaagca ttcgtcgccg cccgcgacgc gtgcgaatac
    10981 gaagaactag ggttccacgg ttggtgtctc ttcgaattga tcgaagctgc gacgcgtact
    11041 ggtgacaggg aaaccgcggc acatgccgcg cagcggctcg aggagcgcac gagccccagc
    11101 gggacggact ggggcctcgg tgccctggca aatgcgcgag ccctgctgtc cgacgatgac
    11161 gtcgctgacg gcctctacac cgaggcgatc gaacgcttgg cgcgcacccg catcgtcgta
    11221 catctcgctc gcacccacct gcattacggt gaatggctgc gccgcatgaa ccggcgcgtc
    11281 gacgcccgcc ggcacctcac cgaggcccat gagatgttca cccggatggg tgcgcaggcc
    11341 ttcgcggaac gcgcgcgccg agaactggtc gccaccggcg agaaggtgcg caagcaggcg
    11401 gtcagctcgg gcaacgagtt gacggcccaa gaggcgcaga tcgcacgtct tgccggcgat
    11461 gggctgacca atcaggagat tggcgcgcag ttgttcatca gcacgcacac cgtcgaatgg
    11521 catctgcgaa aggtcttcgt caagctgggc atcacctcgc gaaggcagct tcgcaccgtg
    11581 tcctgggcca gctaagccag cccgaccagc gactttgtcg tccactacgg actactgact
    11641 acggactacg gacttccaag ggttcgcttc tgctcgccaa ccgcgagtct gatttcgacc
    11701 cgaccgcagc acttggagga ttcccatgac gctcacgtcc gtctccaccc cgtccaagtc
    11761 cgatcaggat ctcgccgcgc agttcgcccg cgaagcggaa cccctctttg aagtcctgtc
    11821 gcgaggtgct cggcgactgg cccgcagcga cgcggacgcc gaagacctac tgcaggacac
    11881 cctgctgcac gcgtacgccg gattccgtac cttccgggag ggcagcaacc tcaaggcctg
    11941 gctcttccgg attctctaca accggtgggt gagcgcctac cgatccaaac agcgccggct
    12001 atcggaggtc tcggtcgacg ggatcactga gcgcgacctg gtcgatagtg cggcgcggat
    12061 accggctggg ctccggtctg cggaggcaga ggtgctagat gcgttgcccg acagcgatat
    12121 caaagccgcg atggaggcgt tgccggacgg cttccgggag gcggtgtatt acgccgatgt
    12181 gcagggatac acctacgcgg agaccgccgc gattctgggc gtaccccacg ggactgtcat
    12241 gtcgcgggtg tcccgaggtc ggcgacggct gcgcatcgcg ctcgcggacg tcgcgtaacc
    12301 gggtcgcgtg caatggaatt aacgaatcag acagcgctgg tgactggcgg aagcgcggcc
    12361 atctgacatc caaccaagga ggaacgaccg atgtcgaaag actatgacgc cgaatgggaa
    12421 aacgcgttga ccgttgtgca ggaagcgcaa ccgcccttca tccccgagga cgcacacgcg
    12481 atgaccgttg tcatcgacta cccaccgggc agtgcgggtg cacccccaca ccggcaccct
    12541 gccggcccgg ccttcggcta catgctggaa ggcgaaatgc tgttcgagct ggaaggtcag
    12601 ccgccgcgcg tggtccgagc cggagaagcg ttctgggaac ccggcggcga cgtcatccac
    12661 tactcggacg ccaacaaccg cgacgacatg aggtgccgct tcacagtcac gatgctctgc
    12721 gtttcaggtc agccgatgct gacgctcgtc gacgacgagg aacttgccgc gcgcaagcac
    12781 ctgcgcgtac ccgccgagcc aaacatgtca aaacccttgt aggagaatga gttccgatgt
    12841 cacgaatctt gctgcaaacg acaatcacgg agagcgccga cgactgggac atcagccgat
    12901 tctcgctgct ggccgacgag ctgcgggccg ccggccacga cgtgaccgca cgcaaccggg
    12961 tcagcagtga cggctccgac cgcgtgctga gccgtctcga cgagatggag tacgaccagc
    13021 tgtggctgat ggccgtcgac ctcggcgatg gcctcacggc cgcagatgcc tactccgtac
    13081 gcaggttccg cgagaacggc ggcggggtgc ttaccgcccg tgaccatcag gacctgggct
    13141 gctgcctgaa ccggctcggg tcgctgggcg tggttaacga attccacgac aagagcgtcg
    13201 atccgagcac gatgtgcgac gaccaagaca ccccgaccat ctcgtggccc aactaccact
    13261 ccggcgccaa cggcgactat cagccagtcc tgatcgaagg accggtgcac gagctgctgc
    13321 gcacaaaccg gacccccagc cggcgcatcg aatggttccc cgcccacccg cacgagggca
    13381 tggtgtcggc gaacggtccc ttcgccaccg cgatcgcgca ggggcgcagc actgcgaccg
    13441 gccgccactt caacctcgcg gtcgtgctcg acggggagct gacgccggat ggccgcccga
    13501 tgggccgggc ggtcgccgaa tcgaccttcc accacttcgc cgactacaac tgggatctcg
    13561 attacggggc accatcattc gtgtccgagc cgcagggcaa tcagatcaag gccgacccgt
    13621 tccgactaga gatcttcaag gactacgtcc gcaacatcgc ggcctggctc cagcccgcca
    13681 acagcctgtc ggtggcggta tgacgcagcc gaccgcgatc gtcactcctt ggcatgggtt
    13741 gcgaagcact ggagatccgc actgtcgctg gcccttccag cgcggtgcag tgggtattga
    13801 gcggtggccc gtacgtcacg gcatcgcagg ttcggcagtg ccagctgacg tgtccgcctc
    13861 cgccgtgtcc cacgcaggcg acgtgcccga cgaggacttg attcgggccg agtgggtgcc
    13921 cgtttgggca gcgggtgggt ggtctggtga tccacctacc ggccgtggtc tgcaccaggt
    13981 cgcccagcac attcgcgatc ctaggtcgat ggatcgaacc tgaggtttca aacgcctgcg
    14041 caaattagct gctggagcta tcgccaacac ctacggccga aacgtggaaa gccaaggcct
    14101 ttcaataggt cagtggccga cgttaaagcc acggccgagg aagtcgtctg ggccggcgcc
    14161 aatctcgcct tcggccttcc aggcttcgcg cctgcgttcg acaagtcgac ggtgggctcg
    14221 gtcgacccgc accatcactg cgaccagtcc agcaagaagc gccagaccga caaaaagaat
    14281 cagagcggct gtcatgacgt ctgacctgca ctcatacaga aaaggtacgc cgctttgctc
    14341 ccgccgcata cagcttctat cgaaagaagt cggcccgccc ctagaaagcg aggggcgggc
    14401 cgaacaatcg gcgtggaccc gcacaaacga acacacacct gcgtggcgat cgacagccga
    14461 ggacagccac ttggcgagaa gaccgtgcag gccacaagcg caggcaacgc ttcggcgctg
    14521 aaatgcgcga ggttgaagtt cgggcctgaa ctcacgtggg caatcgagga tgtgcgaacc
    14581 gtatccggcc gcctggagca agagctaatg agcgcggggc aatcggtggt gcgggtaccg
    14641 acgaagctga tggcccatac ccgtgcctcc gctcgcaccc gaggcaaatc ggactcgatc
    14701 gacgcgaccg agatcgcacg cgcggcgctg cgagagccgg acttgcctgg ggcacagcat
    14761 gatcccgtgt cgcgagactt ccgactgctc acggagcgca gggacgtgct ggtcgagcag
    14821 cgcaccgcta cgaccaacag agtgcagtgg cggatacatg aacttgaccc cctccggtcg
    14881 agaaacctca agccactgat attcgccaaa aaccgtgaac cggtcgcggc gtggctggcc
    14941 gaattgcccg gattggtggc cgaactcggt tgtgacgagc tctccgcatc ccggtgtggc
    15001 gagcacgtgt ttcgcataaa ccgcgtgttc gacaatccgt ttctccagcg cattgatttc
    15061 ctgcgttagc cgcgcaatgt cattgagact caccgcagcg aagatcgttg gtgagaccgc
    15121 caatgcgagt cgtttcaaaa gcgaagcggc tttcgcgcgc tacatcggcg tcgcaccgct
    15181 tccccactca tcgggagcga cagatggtca cctgcgcatg acgcgctcgg gcaaccgtca
    15241 gctcaacgcc gcgatacagc gcatcgcggt tacccaagtt cgcatggatg ggccgggacg
    15301 tgcctattac cgcaaacggc gccaagcggg cgattcgtca ccggcggcgc tgcgcaactt
    15361 gaagcgtcgc gttaccaggg tcgtgttcgg ggcgtctgaa ggccgacgaa gccgaccgtc
    15421 ctccaggtgg cggccacctg gtttctcagt gctagcagtc cgacaagttc ggcagcgcga
    15481 agctcgctgg tgtggccagg cgggacggtc agtttctctc ccggctccga tcgtcttggg
    15541 tcggaaaata ctcggcgagc gtagcggcga tctcgacgaa gcgacgcctc gtggcgggca
    15601 acaactccgt cgtgcggtcg atgacaccgc cgggccgcag atgcccgtcg aacggcacct
    15661 cgataaccgc ctgaccgtgt gagccgaact gctgagccag gatcgaccga gtccgtttgt
    15721 cggcatgccc atcggaatcg ttgagcacga tcacggtgcg ccgcaacaga cctatcaatc
    15781 cgcgggatgc cagccagtcc atcgtctgac cggcggccgc cgcgccgtcg atccacggtg
    15841 acgacaccac gatgagcgcg tcgagatccc gcagtacctc ctgtgtgaca ggagagtcca
    15901 tggtggaact gcaatcgatg atcgagatcg tgaagtagcg gtccaaccgc gacgtcgcct
    15961 cccgatagat cgccgggtcg aggacgcggc ggcgagcagg tgtggcctcc ccgaccagga
    16021 cgaacaagcc agcggcgttg ttgcccaccc gggcgcgcac gtcggcaaac gactccagat
    16081 actggtcgga cgccagctcc cagtaggaac ccggagcctt cgggtcgacg cggctgccca
    16141 gtttgccgaa cgcggtgtcg gcgtcgatag ccaccacgcg gtcgtcgtag cgcagctccg
    16201 cgaacacgga cccaacgcag gcggacacgg tcgtcttccc gacgccaccc ttgcccatca
    16261 cgccgacctt atagtggccg cgcagcaccg atttgatcct cgcctccaac tcggctagtc
    16321 ggatctcgtc ggcagacggg ccaacatgga ccagcccgaa ggtggccttg tagagcgcca
    16381 gccgccagcc gcggatcgga gggacgcggc gcgcgggcca caattcgtcg acgcggatcc
    16441 cttcggcata cgaggcgggc ggcgcaggct gcgcaccggt atccggcccg tggcggggac
    16501 ccggaaaccg ttgacctgtc cacccgccgg gcaccggcgg cggttgcgcc ggctgcggtg
    16561 cacgcggtac ctgcgagccg agttgccctc ccgcctcgcc tgtctcgacg cgactggcag
    16621 cgggcggagc gttctggagc gcgccgggcg ccggaggctg tgggtgcgga cctacttgcg
    16681 gcggttcgcg gaagctgccc cgcgatcgat cagggtcaac gaagagaggc gggccctctg
    16741 ctggacccag acgggagcct tcaggttgag gtggtagtgg cggcggctcg gcccgcaacc
    16801 gatgcggtcc cgtggagaac ggccggggct cgtccgcgcg tgtgggctcg cccgcgcgtt
    16861 cctgcggctc tggtggaacg aaatccatcg gaggcctgcg cggcaacgcc gagtcggcgg
    16921 aaggcgacgg ttcggccggc ccgggcggtc ctgtctccgg gtcgtggtcc gactcctccg
    16981 gacccgtcca cccaggctgt cgccgcaagg cgtcgtcgcg gtcggtcact gcaaattttc
    17041 cctccagctg tcgaacgacg cgaatgtcgg ccactctcaa agtacgcctg gcctagggtt
    17101 acctgcgctt gttcactctt ccgacgcggg ccacaccttt ctgccacccg cgtcatcgcg
    17161 gcgaggtcag cgcaccgctt caagcaccca cgcgaccccg tcgagcgcat ctagcacggc
    17221 tgggcggccg accgcagcca tcagcaattc ggctgcccgc ccatggatct cctgcccttt
    17281 ccccacgttc ggtccatgtc ggtggcatgc aaccgaatcc ctcgcagccg tcccagcggt
    17341 acgagaccga ttggcacggg gccggtcaag aagtccaacg cgactgggcg accgcgagat
    17401 ccctgtgatg gtgctcgaac cctctggagc cgcagcggtg gcatcctggc ttgatggggc
    17461 tgctgctcgt ccgactcgtc gagttgctga tcgtcatcgt gccggtgatc ggcctggtcg
    17521 tcgccggtat gaaagcggtt tcggctgccc gggagcgtca ggcacacgga ggcgacgaat
    17581 cggtgcccca ggccaacacc caggcggcgc agtggcgggc catctcgcgc acggtcaagg
    17641 agcatgaccg aaccgacacc cggtggttgg attacgaact cgacatcggc aagctgctcg
    17701 actttccgct gatgaccgac atgcgtaacc cgctcaccga gcgcttccat cgggcgaaac
    17761 tgcgagccga cctgctacgg ccggccaagg ccgaagatct tctcgaagac ggcgacgccg
    17821 cccgtgagta cctcgacgcg gtcgagaact atgtgacggc attcgatgtc gctgagagcg
    17881 aggcgattcg caggcggcgc aacgatttca cgaaacctga gcagcaacgt ctcacccgtg
    17941 cgcggaatgc tctgcgggtg gcggtggatg ccggcgcgac cctgcaggag cgcgaacgtg
    18001 cctacgcgct tgcctcgcgg gaactcgacg ggctgattgt gctgcccgag cgtgctcgag
    18061 ccgccatcga gcgcggcatc ctcggtgagc tcgacggcta gctatgccgc ccggccgttc
    18121 gacagcaccc gacgcgccgc gaagtccgcg gcctgaaccg ccatcccgtt cacggagtac
    18181 agattgtgcg caccgttgtc gttgccggtg ggcgagcaga cgttgtcctc ggggatgcac
    18241 aggtccgtgg tcttatccgc atagaggtgg ccgaccgtga tcggcggcgc tccggtatag
    18301 atcatctgca ggaacccgct cgatggcttt ccgaacagaa ccaccgcggc gacgtgcttc
    18361 gccacctccg gcgccatcgg gccggtaagc ccggccggaa gggtgaagcc gttgggcact
    18421 gagtcctcgg tcgtataggc gacgaccgcg gccccctggg aatacccgcc gagcaccatc
    18481 ttcgtgctcg ggcagctcgc ggcgacgtcg cggatcttgt tgctcgcgtc gacgacccca
    18541 tcggcggccg tcgcgaaatc cagcgacgcg gggtaattga ccgggtacac atccatagat
    18601 ttgtcgccga gttgaccgcg cagggcatcg acgaaggcct gtcctgtgcc tcccacaccc
    18661 ggtgcctcga acgtgccgcg tgcgaatatc acctcgacgt cggggcatgg ttcagcagag
    18721 gcggtgggaa tgttgatcgc agtgcccaga agcgccgagg tcgccaccac cgccgcaccg
    18781 aaaagtcgat ccattgcgct catcaaaacc tcaccgcccc atatagttcc cgacccggcg
    18841 caggctccgg tgcccgctcc gcagatgccg ccgggtaccc agcggcgcac ccgaccaaac
    18901 caagatcata attgccgccg cggctattcg gtactgagat atgcgtccgc ccatgcgccg
    18961 atgattcgcg ctgcccgacg ggcctggccg ggtccggtca acaagtgctc tgaaccctcc
    19021 agggagacga agctccgcgg atgacgcgcg gtgcggaaga tttcgctcgc gttctcgatg
    19081 ccgacggtgt tgtcggtggg cgagtgcagg atcagcagcg gcatccgcag gccgcggatc
    19141 ttgtcgtgca gagctgccgc gcggacgtcc tccacgaacg cacgcttgag cgtgagcgtg
    19201 cgcccgccag ccatccactc ggcgctgcct tcggacaaca cgcggtccac caccgcgtcg
    19261 tagtgctgct cggcgtgcga cgggtccacg ggcgccccga ccgttgccac cgcccgcaca
    19321 cccggcgcct gccgggccgc ggcgatcacc gccgcacccc cgaacgaatg gccgaccagg
    19381 atgtcggcgg gcgtcccttg ttcggccatg aactcgcacg cccgaacgac gtcgttgacc
    19441 ttgacggtga acgaaccgtc gccccagtcg ccctccgagt cgcccaaccc gagcgcgtcg
    19501 aatcgcaaca tgccgatgcc gtcctccgcg agctgcttgc agatgcgagc ggcggcaggc
    19561 gagtccttgc caagcgtgaa cccgtgcgag aagacgcccc agccgcgggg ttccccggct
    19621 ggccggtcga tgattcccgc caggctcggc ccagtcgagc ttccgaacgt cacgcgctca
    19681 gccatgccgg aggtttacca cctgcggagc ccgattgacc atgtcgctac cgtttaggca
    19741 tgtccgaaca ggtcaccacc cacgcgcccg atccggccga tgtcggcaag cggatcgacc
    19801 cggtactcgc ccggagttgg ctgttggtca acggcgcgca tgccgaccga ttccaggccg
    19861 ccgcccactc gcgcgccgac atcgtcgtgc tcgacatcga ggatgcggtc gccccgaagg
    19921 acaaggccgc cgcccgcgac aacgtcgtgc gctggctcga ggccggaaac tccgactggg
    19981 tccgcgtcaa cggtttcggc accccgtggt gggccgacga cctgaaggca ctggcaagca
    20041 cgtcgatcgg cggcgtgatg ctggcgatgg tcgagtcggt cgaccacgtc accgagaccg
    20101 cgaagcgtct gcccaacgtt ccgctcgtgg cgctggtcga aacggcgcgg gggctggaac
    20161 gcatcaccga gatcgccgcg gcgaaaggga ccttccggct cgcattcggc atcggcgact
    20221 tccgccgcga caccggcttc ggcgaggacc cggccacgct cgcgtatgca cgctcccgtt
    20281 tcacgatcgc ggccaaggcg gcccatctgc ccagcgcgat cgacgggccg accatcggct
    20341 cgaacccgct caagctcatc gaggccacgg ccgtttccgt cgagttcggg atgaccggca
    20401 agatctgcct cacgcccgac cagtgtgtag tcgtcaatga gggactttcg ccgtcgcagg
    20461 acgaaatcgc ttgggctaga gaatttttcg ccgaattcga gcgcgacgga ggcgagatcc
    20521 gcaacgggtc ggatctgcca cgcatcgcca gggccaccaa gatcctcgag ctggctcgct
    20581 cctacggcat ccaggtttcc gagttcgacg acgagcccgt gcacatgccg gccccgtcgg
    20641 acacctacca ctactgattg acgtcttcgc gcacacgaag ctggggattc cccggaccaa
    20701 tcggctgtct cgttgctcat gtcggcaacg ccgcgggccg gttggcggtg gacgttcacg
    20761 ccgaccggct ctgtcgcttc gcggccctgc gggccttgat gcgcgccact gacctccgcg
    20821 acggcggatg tctgcggcct cgccggttaa ttgatgcttt ccgatcggac ctcggcgacc
    20881 catcaacgtc ctgacggcgg agcagccagc catggttggc tgcccccagg tccatccggt
    20941 cgagaccatc agtccgcctg tggacatgca gtcccaggta ggcggagaac aggtcccgat
    21001 gttcgaggac gccgcactca cagcggtgaa cccgctggga gagcgtcttc ttcttgcggt
    21061 tcccgcacag gcaggtttgg gacagggccg tggtgtcggg gttgtactcg tagcggcgat
    21121 tacccccggc gctgtcagcc ttgcggcgca tcatctcaac gagcagccca ggtgcccggt
    21181 cccgtacgct gcgcgggaag ttcttctgcc atgcgccgta accgagcttt tcgcaggcaa
    21241 tgtcaacacc atgggcgaac agccggtttg ccagggcccc aacacagttg tgacaggtgt
    21301 ggtcaggtgc cgcagaaggt tcggtaggcg ccgaagtcct cgggtgccgg ctcggcgtag
    21361 cggtcgaatc ctggtctttc gtcgtagggc gcggtcacgg cggcaagcag ctgcgtgagc
    21421 ggatcgacat cgccgtcggt cgcggcggcg agggcctcct cgaccaagtg gttgcgtggg
    21481 atgtacaccg ggttgacccg gtccatcgcg tccgcgtccg gaatcagcgc ccgccagcgg
    21541 gcgagccaac cgtcgaacgc ggcaaggtcg acgaacagtc cgcgcgccgg ttcgacgtcg
    21601 ccgcgcgccg cccggccgag caggcggtag aaggacgtgt ggtcgatgcg gctctgctcg
    21661 agttgttcga gcagctcagc gatcagcgga gtcgcgacgg cgtcgtcgac gtcgcgcagc
    21721 ccgagcttcg ctcgcatgcc tgccatcgat gcgtcgacgt agtgccgccg gaagccgtcg
    21781 agcgactcct gcgccagagc gagcgcgtcg tcgagatcgt cggcgaacag cggaagcaac
    21841 gtctcggcga accgggccag gttccacgcc gccaccgccg gctgttggcc gtaggcgtat
    21901 cggccccagc tgtcgatcga gctgaacacc gtcgccgggt cgtaggcctc catgaatgcg
    21961 cacggcccgt agtcgatggt ctctccggag atcgtcatgt tgtcggtgtt catcacgccg
    22021 tgcacgaacc cgacgagcat ccattgcgcg atgagcgacg cctgggcggc gacgaccgcc
    22081 tcgaacagcg cgcggtatgg attctccgcc tccgcggcat tcggatggtg ccgggcgatg
    22141 gcgtggtcgg cgagccgccg caccaggtcg gcgtcgcccg cggtggcggc gtactgaaag
    22201 ctgcccaccc gcacgtggct gctcgccacg cgcgcgagca cggctcccgg caacagcgtg
    22261 tcccgatgga cgcggcgacc cgtggccaca acggcgagcg accgggtggt ggggatgtca
    22321 agcgcgtgca tcgcctcgct gatgacgtac tcgcgcagca tcgggccgac caccgcgagc
    22381 ccgtcgccgc cgcgcgcgaa cggtgtgact ccggacccct tgaggtgaat gtcgcgaacc
    22441 gtcccgtcct ggcaggcgat ctcgccgagc agcagggcgc gtccgtcacc cagacgcggc
    22501 acgaagccgc cgaactggtg tccggcg
//
LOCUS       DASRJA010000139         3870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3555048,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000139 DASRJA010000000
VERSION     DASRJA010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3555048"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFC01_06430"
     CDS             <1..386
                     /locus_tag="VFC01_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79310.1"
                     /translation="VIVAILAVGPIAVAASMFPRYAREGGPVANVLYRICQTYAVVGV
                     AVPPLGIATAVSMGVLSQAWVLVSIALTAVAALVLVASILPDQRAALNGVVVTRLAMT
                     TGVFNLLWVVVTVLMIVRPGSTTGV"
     gene            complement(383..1264)
                     /locus_tag="VFC01_06435"
     CDS             complement(383..1264)
                     /locus_tag="VFC01_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN79311.1"
                     /translation="MTAQPGFPDLDGVEHRYVDLPGLRMHVAEAGSGEPLLLLHGFPQ
                     HWWGWYKVLPALAQHYRVIAPDLRGAGWTDAPASGYTAEQLVADVVALLDALELDRVR
                     LMAHDWGALVGFLLCLYHPERVERYVSLAIPHPFVKFSPRLLAAVPRLWFQNAIATPG
                     LGPYLLGKGRQPMATYLLDHYTSDRSAFTPRDRELFVAPFRDPARAHAGSALYRHFIV
                     PLVPRIMSGHYRKMRLTTPTLILYGREDPNMNTTILDGHQGYADDLTLEEVDGASHFI
                     ADEKPGVVIERALEFFA"
     gene            complement(1261..1875)
                     /locus_tag="VFC01_06440"
     CDS             complement(1261..1875)
                     /locus_tag="VFC01_06440"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZN79312.1"
                     /translation="MTVEARDAKRKLPAERHGELLAKAVEISAKEGLSAVTLRRVAAD
                     LGVTPGLVSHYFSSAEQLITAAFQSAAVADLDEARARVDTARTAVAKMDALMDYVLDD
                     SSDDASALWLEAWSLGRRNAPLAAEAKELTDEWLACIAGIVRAGVAEGAFHVADVDVA
                     ARRLLTMIDGLGAQKVVRAVPTDEAKHIAQSYFASELRVAGSTS"
     gene            1959..3413
                     /locus_tag="VFC01_06445"
     CDS             1959..3413
                     /locus_tag="VFC01_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014985309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HZN79313.1"
                     /translation="MTATLPRPETTFTADRIGAVEDAGVEYLPEDARDSRPRNLGAVF
                     LGANLTWTNVVFGAFAILFGLSFWQTVTSMAVGIAVGTLAVLPTAIIGPRTGTNMTVS
                     SGAFFGIRGRFIGSGIALLIAFGFAAVTVWTSGDAIVAAGHRLLGTPENDVVHAVAYA
                     AVAALMVTVALYGHATIVAMQKVVVPVVGALMILGVFAFAGGFDPNASTGEYMLGGFW
                     QTWALSAVLFAAAPISYGPTIGDYTRRISARRFSNRRICSALGVGMFVGVLLPSLFGA
                     FTAMTFTTPTDSYLDDLVAAAPGWYVLPIVVISLLGGLSQGVLCVYASGLDLEGLAPR
                     LKRTQTTIVTAAVAIALLYLGVFVFDAVDSVTAMTVALNAVITPWVAILAIGALRTKV
                     YDPTDLQAFAQGRHGGRYWFTGGWNVPAVAAWTVGAVFGLLAVNTTLYVGPLADIAGG
                     VDVSTVGSAALAGLLYVAAQAVRPAAAPRHVACR"
     gene            complement(3373..>3870)
                     /locus_tag="VFC01_06450"
     CDS             complement(3373..>3870)
                     /locus_tag="VFC01_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79314.1"
                     /translation="GWTGSVNHNWGTRHTPAYAFGQVCGFDDAPDSSLEIVTARAAVG
                     PLLTPAATLFVFRHAGQEFAVRSILGSLQTHGKYKPFSWAFGGRVGDQMIEGEIITQP
                     ADVIGLTYTDTDGGTKFCYNSAIATCRVQVAGKAFERGELVATRRAMLEILTDKQHDE
                     VPLLA"
BASE COUNT          605 a         1316 c         1263 g          686 t
ORIGIN      
        1 atgtcatcgt cgccatcctc gccgtcggcc caattgccgt cgcggcgagc atgtttcccc
       61 gctacgcacg cgagggcggc cccgtcgcca acgtgctgta ccgcatctgc cagacgtacg
      121 cggtcgtcgg tgtggcggtt ccgccgttgg gtatcgcgac ggcggtttca atgggcgtgt
      181 tgagtcaagc ttgggtgctt gtctcgatcg cgctgacagc cgtggccgcc ctcgttctcg
      241 tcgcgagcat cctgcccgac caacgcgccg ccttaaatgg cgtggtcgtg accaggctgg
      301 ccatgacaac gggcgtcttc aacttgctgt gggtggtggt cacagtcctg atgatcgtgc
      361 ggccagggtc gacgactggc gtctaggcga agaactccaa cgcccgctcg atcaccacac
      421 caggcttctc gtcggcgatg aaatgcgaag cgccgtcgac ctcttcgagc gtcaagtcgt
      481 cggcatagcc ttggtggccg tcgaggatcg tggtgttcat attggggtcc tcgcggccgt
      541 acagaatgag cgtcggtgtg gtcaggcgca tcttccggta atggccggac atgatccgcg
      601 ggacgagcgg cacgatgaag tggcgataca gcgccgaacc cgcatgcgcg cgagcgggat
      661 cgcggaacgg cgccacaaat agttcgcggt cgcgcggcgt gaacgcgctg cgatcggacg
      721 tgtaatggtc gagcaggtac gtcgccatcg gctgtcgccc cttgcccaac agatacggcc
      781 ccaaccctgg cgtcgcgatc gcgttctgaa accacagcct cggcaccgcc gccaacaggc
      841 gcgggctgaa cttcacgaac gggtgcggaa tcgccagcga gacataccgt tccacgcgct
      901 ccgggtggta cagacagagc aggaaaccga ccagcgcgcc ccagtcgtgc gccatcagtc
      961 gtacgcggtc gagttccagc gcgtcgagca gcgcgaccac gtcggcgacc agttgctcgg
     1021 cggtgtaacc ggaagcgggc gcatcggtcc agcccgcgcc tcgcagatcc ggcgcgatca
     1081 cccgatagtg ctgcgccaac gccggcagca ccttgtacca cccccaccag tgctgcggaa
     1141 acccatgcaa caacagcagg ggctcaccac tgcctgcctc ggcgacatgc atccgcagcc
     1201 cgggcaggtc gacgtaccgg tgctcgacac cgtcgagatc cggaaacccg ggctgcgcgg
     1261 tcatgacgtc gaccctgcca cgcggagctc cgacgcgaag tacgactggg cgatgtgctt
     1321 ggcctcatcg gtgggcaccg cacgcacgac cttctgcgcg ccgagcccgt cgatcatcgt
     1381 caacagccgg cgcgccgcca cgtcgacgtc agccacgtgg aacgctccct cggcgacgcc
     1441 cgcccgcacg atgccagcga tacaggccaa ccactcatcc gtcagctcct tggcctcggc
     1501 cgccagcggt gcgttgcgcc gacccaggct ccacgcctcc agccagagcg cgctcgcgtc
     1561 gtcgctggag tcgtcgagca cgtagtccat cagcgcatcc atcttggcca ctgcggtccg
     1621 cgcagtgtcg acccgcgcac gggcctcatc gaggtcggcc acggccgccg actgaaacgc
     1681 ggcggtgatc agctgttcgg ccgacgagaa atagtggctg accaggccgg gcgttacacc
     1741 gaggtcggcg gcgacgcggc gcagcgtgac tgcggacaac ccctccttgg cggagatctc
     1801 gacggccttg gccagcagtt cgccgtgccg ctcggctggg agcttccgct tcgcgtcacg
     1861 cgcctcgacg gtcaatttcg gtcctcctat tgacgaatgc cgatccttat tgaacactac
     1921 atcaacacta ttgatctatc catcaacaag gatccgccat gaccgcgacc ctgcctcgcc
     1981 cagagaccac cttcaccgcc gaccgcatcg gtgccgtcga ggacgccggc gtcgagtacc
     2041 tccccgaaga cgcccgagac tcgcggccgc gcaacctcgg cgcggttttc ctcggcgcga
     2101 atctcacgtg gacgaacgtc gtgttcggtg cgttcgcgat cctgttcggg ctgagtttct
     2161 ggcagacggt cacctcgatg gccgtcggca tcgcggtcgg caccctggcc gtcttgccca
     2221 ccgcgatcat cggcccacgc acgggcacga acatgacggt gtccagcggc gcgttcttcg
     2281 gcatccgcgg ccggttcatc ggttcgggca tcgcactgct catcgcgttc ggcttcgccg
     2341 cggtcacggt gtggacaagc ggtgacgcga tcgtcgcggc cgggcatcga ctgctcggca
     2401 cgcccgagaa cgatgtggtg cacgccgtcg cgtacgccgc ggttgcggcg ctgatggtga
     2461 ccgtcgcgct gtacggccac gcgaccatcg tcgcgatgca gaaggtggtg gtgcccgtcg
     2521 tcggcgcgct gatgatcctc ggcgtgttcg cgttcgcggg cggcttcgat cccaacgcgt
     2581 cgaccggcga gtacatgctc ggcggcttct ggcagacgtg ggcgttgtcg gccgtgctat
     2641 tcgcggcggc gccaatctcc tacgggccga caatcggtga ctacacccgc cgaatctccg
     2701 cgcggcgctt cagcaatcgg cgcatctgca gcgcattggg cgtcggcatg ttcgtcggcg
     2761 tcctgctgcc cagcctcttc ggtgcgttca ctgcgatgac gttcacgacc ccgaccgatt
     2821 cgtatctcga cgatctggtg gcggccgccc cgggttggta tgtgctgccg atcgtggtca
     2881 tctcactgct gggcgggctg agccagggcg tgctgtgcgt ctacgccagt ggcctggatc
     2941 tggaaggcct tgcgccgcgg ttgaaacgga cacagaccac gatcgtcacc gccgcggtcg
     3001 cgatcgcact actgtatctg ggtgtgttcg tcttcgacgc ggtcgattcg gtcaccgcga
     3061 tgacggtcgc gctgaacgcc gtcattacgc cgtgggtcgc catcctggcg atcggcgcgc
     3121 tgcgcacgaa agtctatgac ccgacggatc tccaggcgtt cgcacagggc cggcacggcg
     3181 gccggtactg gttcaccggc ggctggaacg tgcctgccgt cgcggcgtgg acagtcggcg
     3241 cggtgtttgg actgctggcg gtcaacacaa cgctctacgt cgggccgctc gctgacatcg
     3301 caggcggggt cgacgtgagc acggtcggat cggcggcgct ggcggggttg ctttacgttg
     3361 cggcgcaagc agttaggcca gcagcggcac ctcgtcatgt tgcttgtcgg tgaggatctc
     3421 gagcatcgcg cggcgggtgg ccaccagctc gccgcgttcg aaagccttgc ccgccacctg
     3481 aactcggcac gtcgcgatcg ccgagttgta gcagaacttc gtgccgccgt cggtgtccgt
     3541 gtaggtcagc ccgatgacgt cggccggctg ggtgatgatc tcgccctcaa tcatttggtc
     3601 ccccacccgc ccgccgaacg cccacgagaa cggcttgtac ttgccatgcg tctgcaggct
     3661 gccgaggatc gagcggaccg cgaactcctg gccggcgtgg cggaacacaa acagcgtggc
     3721 tgcgggcgtc aacagtggtc cgacagcggc ccgcgcggtc acgatctcca gacttgagtc
     3781 gggcgcgtcg tcgaatccgc acacctggcc gaacgcgtaa gccggcgtgt gccgcgtgcc
     3841 ccagttgtga ttgacgctgc cggtccagcc
//
LOCUS       DASRJA010000140        12442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2427514,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000140 DASRJA010000000
VERSION     DASRJA010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12442
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2427514"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VFC01_06455"
     CDS             <1..305
                     /locus_tag="VFC01_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN79315.1"
                     /translation="KPRIIASVPVLVSDDADAARVEAAERLAFYETIPSYQKVIARED
                     VASAADLAAVGTPEAVARKLSSYRDAGATDLALSPIRRDDKALHDIWDIARNIDAT"
     gene            324..1049
                     /locus_tag="VFC01_06460"
     CDS             324..1049
                     /locus_tag="VFC01_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN79316.1"
                     /translation="MPPRRRSVLIARLTVDSPGRPQEAILDELRRVILDGAVLPGTPI
                     PLAEVAELFGVSQIPVRESLKTLIGEGLVTHRSNFGYTVAQLTPQELREMYIVRETLE
                     AAALAASVAKATDEDRATIVAVNDILQQAIRDDDPVTYHRQSREFHLALTRPSRMYRL
                     LHMLESAWNVTEPVQSMVHVGPEDRATLHEDHCEMVEVFLSGDVEQLLTIAEIHAQRL
                     NSVIATLPTDTGLLVTPNISSVQ"
     gene            1103..2653
                     /locus_tag="VFC01_06465"
     CDS             1103..2653
                     /locus_tag="VFC01_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HZN79317.1"
                     /translation="MTDLDTSRTSELPPGAVVAAGDLVEAAGHPVGSGDLKPGYDPRL
                     TNEDLAPLRKQTWTSYNIFAFWMSDVHSVGGYVTAGSLFALGLASWQVLVALLIGIVI
                     VNIFCNLVAKPSQATGVPYPVICRSVFGVLGANIPAIIRGLIAVAWYGIQTYLASAAL
                     DVVLLKLFPSLAPYADVNQYGFLGLPLLGWGSFVLLWILQACVFWRGMEAIRKFIDFC
                     GPAVYVVMFLLCGYLIYQAGWGAIDLKLGEVTYTGLDSIPVMLGAIALVVSYFSGPML
                     NFGDFSRYGKSFDAVKRGNFLGLPVNFLVFSLLVVVTASLTVPVFGELITDPVATVAR
                     IDSTFAIVLGALTFTIATIGINIVANFISPAFDFSNVSPQRISWRAGGMIAAVGSVLI
                     TPWNLYNNPEVIHYTLEILGAFIGPLFGVLIAHYYLVHKQKVIVDDMFTLDENGTYWY
                     TKGYNPAAVIATAVGAIVAVVPVLLGGSVVGMHTAAQYSWFIGCGIGFGLYWTLATRG
                     RLAVQPVA"
     gene            2650..3363
                     /locus_tag="VFC01_06470"
     CDS             2650..3363
                     /locus_tag="VFC01_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HZN79318.1"
                     /translation="MTQIWVINPNTTQAMTDTIEACARAVVAPGVSIIGVTSEIGPPS
                     IESHYDEARGVPGVLQAVERGERQGADGYVIACFGDPGLDAAREFATGPVIGIAEAAM
                     QTASHLGRGFSVVTTLARTMGRAADLAERYGMHRFCLGIHACELPVLDLDTDPNARKI
                     VTEACREAVESDGSDVVVLGCAGMANMCASISAELRVPVIDGVTAATLTVQSLVAMGL
                     GTSKRSEFAPPVPKDYSAG"
     gene            complement(3348..4250)
                     /gene="puuE"
                     /locus_tag="VFC01_06475"
     CDS             complement(3348..4250)
                     /gene="puuE"
                     /locus_tag="VFC01_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HZN79319.1"
                     /translation="MSYPRDMVGYGRTPPDPKWPGGAVIAVQFVLNYEEGAENSVLDG
                     DPASETFLSEMTPAEAFPNRHMSMESLYEYGSRAGLWRLLRVFERRGIPLTIFAVARA
                     MQRNPEAVKAFGELGHEIACHGLRWKSYQLVDRDTERADMAEAVEILRELTGTAPLGW
                     YTGRDSSHTRELVVEHGGFVYDSDSYADDLPYWVRVNDTDHLVVPYTLDTNDMRFASA
                     AGFSNGDEFFAHLRDAFDVLYAEGRNGSPKMLSVGLHCRLAGRPARTKALERFCDHVQ
                     SHDGVWFARRIEIAQHWRTVHPAE"
     gene            complement(4247..5218)
                     /gene="alc"
                     /locus_tag="VFC01_06480"
     CDS             complement(4247..5218)
                     /gene="alc"
                     /locus_tag="VFC01_06480"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HZN79320.1"
                     /translation="MADSQDSQPDFTWLPDLALRPLGGAVLWANDETFAEKENLINQG
                     PSTFRPASFGHKGQVYDGWETRRRREPGHDAAIVRLGVPGKIRGIVVDTAFFKGNYPP
                     EVSIEAIEIDDYPRAEQLAADPRWETVVERAKVSGDTRNPFEVSSEKRWTHVRLTIYP
                     DGGVARLRVHGEGIPDPRFLGTGPVDLAALENGGLVLDCSNRFYSSPQNIIFPGVAQV
                     MGDGWETARRRDDNNDWVHIRLAGAGVVRIAEIDTSYFIGNSPGAAALSGLRPDGEWV
                     ELLPRTELLPDTRHRLLVEPKHIVEQARLDIYPDGGLARLRLFGDLA"
     gene            complement(5239..6411)
                     /locus_tag="VFC01_06485"
     CDS             complement(5239..6411)
                     /locus_tag="VFC01_06485"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZN79321.1"
                     /translation="MTVRRLEPYAVTIFAEMSALAARIGAVNLGQGFPDEDGPPSMLK
                     VAENAITEGVNQYPPGLGIAPLREAIAAQRQRHFGTEYDPDTEVLVTVGATEAIASAV
                     LGLVEPGSEVLLIEPFYDSYSPVIAMAGCGRGAVPLIQDGRGFRIDVDGLRNAVTPKT
                     KALIVNSPHNPTGMVASDDELRGLAQLAVDADLLVITDEVYEHLVFDGHRHIPLANYP
                     GMAGRTVTISSAAKMFNCTGWKIGWACGAADLIAGVRAAKQYLSYVGGAPFQPAVAHA
                     LNTEEAWVAALRESLQAKRDKLGSALADLGFEVHDSFGTYFLCADPRPLGYHDSTEFC
                     AQLPERAGVAAIPMSAFCDPGAEHAGAWNHLVRFAFCKRDDTLDEAIRRLGVLRRP"
     gene            6567..7586
                     /locus_tag="VFC01_06490"
     CDS             6567..7586
                     /locus_tag="VFC01_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZN79322.1"
                     /translation="MPRRVTIDDVARLAEVHKATVSRALNARTRDQVNAETLKRVKRA
                     ARQLGYVPNAMARGLRTSRSMTIGVIIPDLMNPIFPPIVRGIEHVLQPRGYTVLVANT
                     DSHDDVEVSAFESLLQRRVDGFILATGRLDDQTVIDEATTAAVPVVLVNRDSGGGRYP
                     LVSGNNSSGIDSAVTHLVTLGHSHIVHVAGPLNFSTTRARAEAFEAAANRAGVRHSTL
                     HAQSLSIEAGCRAADELLASHGERPTALVAGNDLVALGLIRRLRANGLRCPEDMSVVG
                     FNDMPFAEDFWPPLTTVHMPLWEIGAEGARLLLHGIEDGEQEAATLTLPVSLVVRGST
                     GPAPA"
     gene            7713..8924
                     /locus_tag="VFC01_06495"
     CDS             7713..8924
                     /locus_tag="VFC01_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HZN79323.1"
                     /translation="MRKITALRAIVGIAAIALAASGCTADGPANSDTATIGASLPLTG
                     EFSQGGLDTQRGYETWVEMTNEGGGLVGKPVKIIVKDDATTQDTAVSNYNNLIAKDKV
                     DLLLGSQSSLLNIPASAVAEKNKMLFVCPSCASPDMFSRGFSYIFFSQQAVASDQAKV
                     FAHWIANLPPDQRPKTAAYPALDDPFAGPAVDGAEKILSAAGIRTVYKDQYPTTTKNF
                     DSVVNAMKDAGAEMVVQGAQFEDGVNMIRAMNRAGYKPSIVFQSSSPTYGQQYLDGVG
                     AQNAEGVFSSASYSPIAKTADNAEFVKRYEQKYGTTPPEDAADGFAAAQVLAAAVNAV
                     GSYDDQAKLADWLRANIVETVLGPLSWNADGSPKGEFLVGQWQGGVSQVVLPTDVATT
                     DKILRWQGGVI"
     gene            8954..9835
                     /locus_tag="VFC01_06500"
     CDS             8954..9835
                     /locus_tag="VFC01_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN79324.1"
                     /translation="MVSLVQTLILGLLVGALYALMASGLTLIFGVMRVINLAHGAFAI
                     LAAFLTFTLWRNLGMDPILSIPVVMAVMFAIGWLVYVLVIRHVRGAHVSMTVLATFGI
                     ALVLEGAMGFIWGNTSTAVRTSYTDASLSLGPLYVPQAMLITAAIAALVIGGLYLLLN
                     YTWTGRAIRAASTNEGGALLVGVSVATVAALTFAIGVATLGAGGAMLSTIYPFLPGTH
                     YQWIARLLAIVVLGGLGSLPGAILGALVIGVGEMAATNYLGAAWPVAVPFLVIIVVLI
                     TRPQGILGTRLREDAVA"
     gene            9832..11700
                     /locus_tag="VFC01_06505"
     CDS             9832..11700
                     /locus_tag="VFC01_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HZN79325.1"
                     /translation="MTTISGASRLLDPRMQKLITRGLVLVAAVVIVLFPLSPSASAQN
                     TVILALVLAIGASGLNIITGYTGYLSLSQGAFIGIGAYVGAILATRFPDVQPLLWAPI
                     AGVVAALIAMLLGLVTYRSRGPAFVIITVAFLFLVQFVAIEWVPVTNGTAGLTLPLPD
                     WPDPWGNWPFHLALVVLLALSLTMTWWIRRTKFGMGLIAIREDEGKAGTIGVNAPVYK
                     LLAFGASALFVGMAGSVYGYYLSFVDPIGMFSIITSALIVLSVILGGRGTLFGPVLGA
                     FIIQPVNNFANDTFGGGNARLVIFGGLLVLLVILLPKGILPTAAALLEKTRSKGTVGL
                     SGARLNPLDRPLAPAEARPPAPTGRTLLEVRGLHKSFGGNHAVNDCSFTVPEGSITAL
                     IGPNGSGKTTVFNLISGTMTPDRGEILLNGERIERLSPWSRAHRGIGRTFQITRLFRE
                     MTVLENVVAPLRDFSVRQLGLGAVSGSEAARAEELLEFVGMSAYRDARAGALSYGQQK
                     LVELAQVLMLDPKLILLDEPAGGINPTLIERMGDLIRALNAQGTTFLLVEHNMPFVVG
                     LCDPIRVLARGAVIAQGSPEQIQKDPLVLDAYLGDDYLLEAPSRSVNIPVVERNPA"
     gene            11697..>12442
                     /locus_tag="VFC01_06510"
     CDS             11697..>12442
                     /locus_tag="VFC01_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN79326.1"
                     /translation="MIRLEGVVAGYGGGNVLQGVDLEVNTGELGCIVGPNGAGKSTVL
                     RAVSGLLKPSAGRILLDGEDITGRTPDAVLARGVTQVPQSNGLFATLTVRENILMGAY
                     VIRKQRALVRQRYDEVLDMFPLVQNKTGVRCGNLSGGQRRMVEFARSLMLDPKLVLLD
                     EPSLGLDPKSLTVIDEAVAIMRAKGKAVLMVEQNVRFGLRMATSGIVMESGRVLLTGP
                     AQSVLSNPEIADLYFGGAVHAPVQSDTAAN"
BASE COUNT         2084 a         4083 c         4030 g         2245 t
ORIGIN      
        1 cgaagccgcg gatcatcgcc tccgtgccgg tgctggtgtc cgacgatgcc gatgccgcac
       61 gagtggaagc ggctgagcga ttggccttct acgagaccat cccgtcgtat cagaaagtca
      121 tcgcgcgaga ggacgtggcg agcgcagcag acctggctgc cgtcgggaca cccgaggcgg
      181 ttgctcgcaa gctgtcgtcc taccgcgacg ccggcgccac cgatctggcg ctctcgccga
      241 tacgtcgtga tgacaaggcg ctccacgaca tctgggacat cgcccgcaac atcgacgcaa
      301 cataggctcc ctaccgtgtc gggatgccgc cacgtcgccg ctcggtgctg atcgcacgcc
      361 tgacggtgga cagtcctggc cggccgcagg aggccatcct cgacgagttg cgccgagtga
      421 tactcgacgg cgccgtcctg cccggcacac ccattccgtt ggccgaggtg gcggagttgt
      481 tcggggtcag ccagattccc gtccgcgaat cactcaagac gctcatcggt gagggcttgg
      541 ttacccatcg aagcaacttc ggctacaccg tcgcccagct cactccacaa gagttgcgcg
      601 aaatgtacat cgtccgagag acattggagg cagcagcgct ggccgcttct gtcgctaagg
      661 cgaccgacga ggaccgagcc accatcgtcg cggtgaacga cattctgcaa caggcaatcc
      721 gcgacgatga ccccgtcaca taccaccggc agagtcgcga gttccatctc gcattgaccc
      781 ggccgtcgcg aatgtatcga ctcctgcaca tgctcgagtc ggcgtggaat gtcaccgaac
      841 ccgtgcagtc gatggtgcac gtcgggccgg aggaccgggc caccctgcat gaagaccact
      901 gcgagatggt cgaggtgttc ctcagtggcg acgtcgagca attgctcacg atcgctgaaa
      961 ttcacgccca gcgactcaat tcggtcatcg cgacactgcc gacggacacc gggttgctgg
     1021 tgacgccgaa tatatcttct gtgcaatagc tgggtatttc acaggcaata cgtgcgaaat
     1081 agagcgtgga taccgtccgc gcatgaccga cctcgacacc tcacggactt cggaactacc
     1141 gcctggcgct gtcgtcgccg ccggcgacct cgtcgaagcg gcgggccatc cagtcggcag
     1201 cggagacctc aagccgggct acgacccgcg actgacgaac gaggatctcg cgccgctacg
     1261 caagcagacg tggacctcct acaacatctt cgcgttctgg atgtccgacg tgcacagcgt
     1321 cggcggatac gtcaccgccg gcagcttgtt cgcgctcggg ctggccagct ggcaggtgct
     1381 ggtagcactg ctcatcggca tcgtcatcgt caacatcttc tgcaacctgg tagccaaacc
     1441 aagccaggcc accggcgtgc cgtatccggt gatctgtcgc agcgttttcg gggtgctcgg
     1501 cgccaacatt ccggccatca tccgggggct gattgcggtg gcgtggtacg gcatccagac
     1561 atatctggcg tcggcggcgc tcgatgtcgt cctgctcaag ttgttcccca gcttggcgcc
     1621 gtacgccgac gtcaaccaat acggcttcct cggcctgccg ctgctcggat ggggcagctt
     1681 cgtcctgctg tggattctgc aggcctgtgt gttctggcgc ggcatggagg cgatccgcaa
     1741 gttcatcgac ttctgcggcc ctgcagtgta tgtcgtgatg ttcctgctgt gcggctacct
     1801 gatttaccag gccggttggg gcgcaatcga tctcaaactc ggtgaggtga cctacacggg
     1861 tctggactcc attcccgtga tgctcggcgc gatcgcgctg gtggtttcct acttctccgg
     1921 tccgatgctg aacttcggcg acttctcccg ttatggcaag tcgttcgacg ccgtcaagcg
     1981 cggcaacttc ctggggctgc cggtgaactt cctggtgttc tcgctgctgg tcgtcgtcac
     2041 cgcgtcgctg acggtgccgg tgttcggtga gctcatcacc gatccagtcg cgacagtggc
     2101 acggatcgat tccaccttcg caatcgtgtt gggcgcgttg acttttacga tcgcaaccat
     2161 cgggatcaac atcgtcgcca atttcatctc accggccttc gacttctcca acgtcagccc
     2221 tcagcggata agttggcggg cgggcggaat gatcgccgcc gtcggctccg tgttgatcac
     2281 gccctggaat ctgtacaaca accccgaggt catccactac acgctggaaa tactcggtgc
     2341 gttcatcggc ccgctattcg gcgtgctgat cgcccactac tacctcgtgc acaagcagaa
     2401 ggtgatcgtc gacgacatgt tcacgctcga tgagaacgga acctactggt acacaaaggg
     2461 ttacaacccg gccgcggtga tcgccaccgc cgtcggcgcc atcgttgccg tcgtcccggt
     2521 tctgctgggc ggttcggtcg tcggcatgca cactgcggcg cagtacagct ggttcatcgg
     2581 gtgcgggatc ggcttcggcc tgtactggac gctcgccacc cggggtcggc tggccgtgca
     2641 gcccgtcgcg tgacacagat ctgggtgatc aacccgaata ccacgcaggc gatgacggac
     2701 acgatcgagg cgtgcgctcg ggcagtggtc gcgccagggg tctcgatcat cggggtcacc
     2761 tccgagatcg gacccccctc gatcgagagc cactacgacg aggcccgcgg tgtgccgggt
     2821 gtgctgcagg ccgtcgaacg cggtgaacgt cagggcgccg acgggtacgt gatcgcctgc
     2881 ttcggcgacc cgggcctgga cgccgcccgc gagttcgcta ccggcccggt gatcggtatc
     2941 gccgaagccg caatgcagac cgcgagtcac cttggccggg gtttcagcgt cgtcaccacg
     3001 ctggcccgca ccatgggccg ggccgctgat ctcgcggaac gctatgggat gcaccggttt
     3061 tgcctcggca ttcacgcctg cgagctccct gtgctcgatc tcgacaccga tccgaatgcc
     3121 cgcaagatcg tcactgaagc gtgtcgagag gcggtggagt cggacggctc agacgtggtg
     3181 gtgctgggct gcgcgggaat ggccaacatg tgcgcgtcca tctcagctga gttgcgcgtc
     3241 cccgtgatcg acggcgttac cgccgcgacg ttgaccgtgc agtcgctggt cgcgatgggc
     3301 ctcggcacat ccaagcggag tgagtttgcg ccgcctgtgc cgaaggacta ctccgccggg
     3361 tgaaccgtcc gccagtgttg cgcgatctcg atgcggcgcg cgaaccacac gccgtcgtgg
     3421 gactgtacgt ggtcgcagaa ccgctctaac gccttggtac gcgccggcct gccggccagt
     3481 cgacaatgca ggccgacgga gagcatcttc gggcttccgt tgcgaccctc cgcatagagc
     3541 acgtcgaacg cgtcgcgtag gtgtgcgaag aactcgtcac cgttggaaaa ccctgcggct
     3601 gaggcgaatc gcatgtcgtt ggtgtccagc gtgtacggca ccacgagatg gtcggtgtcg
     3661 ttgacccgca cccagtacgg caggtcgtcg gcgtaggagt cggagtcgta gacgaaccca
     3721 ccgtgctcga ccaccaactc gcgggtgtgc gatgagtccc ggccggtgta ccacccaagt
     3781 ggtgcggtgc cggtcagctc gcgcaggatc tcgaccgcct cggccatgtc ggcgcgctcg
     3841 gtatcacgat cgaccagctg gtaagacttc cagcgcaacc cgtggcaggc gatctcgtgg
     3901 ccgagttcgc cgaacgcctt gaccgcctcg ggatttcgct gcatcgcgcg ggcgacagcg
     3961 aaaatcgtca gcggaatccc gcgtctctcg aagacacgca gcaagcgcca cagccccgcg
     4021 cgggacccat actcgtacag cgactccatg ctcatgtgcc ggtttgggaa tgcttctgcc
     4081 ggcgtcatct cggagaggaa tgtttccgaa gccgggtccc cgtcgagcac cgaattctcc
     4141 gcgccctctt cgtaattgag cacgaactgg acggcgatga cggcgccgcc tggccacttc
     4201 gggtctggtg gcgtacggcc gtagccgacc atgtcgcggg gatagctcat gcgaggtcgc
     4261 cgaacaacct caaccgagcg aggccgccgt cgggatagat gtccagccgg gcttgctcga
     4321 caatgtgttt cggctcgaca aggagacggt ggcgggtgtc gggcagcagc tccgtgcgcg
     4381 gcaacaactc gacccattcg ccgtcgggac gcaacccgga tagcgcggcc gcgccggggc
     4441 tgttgccgat gaagtaggag gtgtcgatct cggcaatccg cacgactccc gcgcccgcga
     4501 gccggatgtg cacccagtcg ttgttgtcgt cgcgccgccg agcggtctcc cagccgtctc
     4561 ccatcacctg cgccacaccg ggaaagatga tgttctgcgg tgagctgtag aagcggttcg
     4621 aacagtccag caccagaccc ccgttctcca gcgcggcgag gtcgacgggg cccgtaccca
     4681 ggaaccgcgg gtcgggtatg ccctcaccat gcacccgcag cctcgcgacg ccgccgtcgg
     4741 ggtaaatcgt caatctcaca tgggtccaac gtttttccga cgacacctcg aaagggttgc
     4801 gggtgtcgcc ggacaccttg gcccgctcga cgacggtctc ccaccggggg tctgcggcga
     4861 gctgttcggc gcgcggataa tcgtcgatct cgatcgcctc gatggacact tccggcgggt
     4921 agttgccttt gaagaaagca gtatcgacga cgatgccgcg gatcttgccg ggcaccccga
     4981 gccgcacgat cgccgcgtcg tgcccgggct cgcgacggcg gcgggtctcc cagccgtcgt
     5041 acacctggcc cttgtgtccg aacgaggccg gccgaaatgt ggacggcccc tggttgatga
     5101 ggttctcctt ctccgcgaag gtctcatcgt tcgcccacag gaccgcgccg cccagcgggc
     5161 gcagtgccag gtcgggcagc caggtgaagt caggttgcga gtcttgcgag tcggccacct
     5221 gaccatcgtc acagaaggtc aaggtcggcg cagcacaccg agccggcgga tcgcctcgtc
     5281 gagggtgtcg tcgcgtttac aaaatgcgaa gcgcaccaga tggttccacg cgccggcgtg
     5341 ctcggcaccc ggatcgcaga aggccgacat cgggatcgct gccaccccgg cgcgttccgg
     5401 cagttgcgcg cagaactcgg tgctgtcgtg atagccgagc ggccgcggat cggcgcacaa
     5461 gaagtaagtg ccgaagctgt catgcacttc gaagcccagg tcggccagcg cggagccgag
     5521 tttgtcacgc ttggcctgta acgattcccg cagcgcggcc acccacgcct cctcggtgtt
     5581 gagcgcgtgg gccacggcgg gctgaaacgg agccccgccg acgtaggaga gatactgctt
     5641 ggctgcgcgc acaccagcga tgagatccgc tgcgccgcaa gcccatccga tcttccagcc
     5701 ggtgcagttg aacatcttgg ccgcgctgga gatcgtgacg gtacggcctg ccatgccggg
     5761 gtagttggcc aatgggatgt ggcggtggcc atcgaacacg aggtgctcgt agacctcgtc
     5821 ggtgatgacc agcaggtcgg cgtccaccgc gagctgcgct agcccgcgaa gttcgtcgtc
     5881 ggaggccacc atgccggtcg ggttgtgcgg cgagttgacg atcaatgcct tggtcttggg
     5941 tgtgacggcg ttcctcagcc cgtcgacgtc gatccggaag ccgcggccgt cctggatcag
     6001 cggcacggca ccgcggccac agcccgccat cgcgatgacg ggggagtagg agtcgtagaa
     6061 gggttcgatc aacagcacct cggagccggg ttcgacgagc ccgagcaccg cagaggcgat
     6121 ggcctcggtg gcgccgacgg tgaccagcac ctcggtgtca gggtcgtact ccgtgccgaa
     6181 gtggcgctgg cgctgcgcgg cgatcgcttc gcgcagcggc gcgatgccaa ggccgggcgg
     6241 gtactggttt acgccctcgg tgatggcgtt ctcggcgacc ttgagcatcg agggcggccc
     6301 gtcctcgtcg gggaagccct ggcccaggtt cacagcgccg atgcgcgcgg ccagcgccga
     6361 catctcggcg aagatcgtca ccgcataggg ctcgaggcgc cggaccgtca tgggtgtcga
     6421 gcgtagcgag acggcgaagc tccgtgcagg tcggagcaac tcgccgcaac cgattgcaga
     6481 gaatcctcgg tagctcgcct cgcggacggt agattgggcg acaggaaccc gggccgccgg
     6541 gttcgctgac tgcaaggatg acgaggatgc cgcgacgggt gaccatcgac gacgtggcgc
     6601 gcctggccga ggtgcacaag gcgacggtgt cgcgtgcgct gaatgcccgg actcgggacc
     6661 aagtcaacgc cgagaccctc aagcgggtga agcgtgctgc gcgccagctc ggctatgtgc
     6721 ccaacgccat ggctcggggg ctgcggacaa gtcgctcgat gaccatcggc gtgatcatcc
     6781 ccgacctcat gaatccgatt ttccctccga tcgtccgcgg catcgaacat gtgctgcaac
     6841 cacgtggcta caccgtcctc gtggccaaca ccgactcgca cgacgacgtc gaagtgtcgg
     6901 ccttcgagtc gctgttgcag cgcagggtag acgggttcat cctggccacc gggcgactcg
     6961 atgaccagac cgtcatcgac gaggcgacga cggcagccgt gccagtcgtg ctcgtcaacc
     7021 gggattcggg cggcggtcgg tacccgttgg tctcgggcaa taacagcagt ggaatcgatt
     7081 cggcagtaac acatctcgtc acgctcgggc acagccacat cgtccatgta gcgggtccgc
     7141 tgaacttctc cacaacgcgt gcccgggcgg aggccttcga ggccgcggcg aatcgggcgg
     7201 gtgtgcgaca cagcacgctc cacgcccagt cgttgtcgat cgaggcgggt tgtcgcgccg
     7261 cggatgagct gctcgccagc cacggcgagc gtcccaccgc gctggtcgcg ggcaacgacc
     7321 tggttgccct cggtctgatc cgtcggctgc gcgccaacgg gttgcgctgc cccgaggaca
     7381 tgtcggtcgt cgggttcaac gatatgccgt tcgccgagga cttctggccc ccgctcacca
     7441 cggtgcacat gccgttgtgg gagatcggcg ccgagggcgc ccgcctgctg ctgcacggaa
     7501 tcgaggatgg cgagcaggag gccgcgacgc tgaccttgcc ggtatcgctg gtcgtccgcg
     7561 gttccaccgg gcctgcgccg gcctgaccga ggtcgccgac ggccgggccg caacccgttg
     7621 acaccccgca cagcgctgtg tcacgattca ctgcaatcga ttgcagaaca tgagcgtcac
     7681 acgccgcagt cggttcgaca gagaggttgg acatgcgcaa gatcactgca ctgcgcgcga
     7741 tcgtcggcat cgccgccatc gcgctcgccg cgtcgggctg caccgctgac ggccccgcca
     7801 acagcgacac cgcaacgatt ggtgcctcgc ttcccctgac cggtgagttc agccagggtg
     7861 gtctggacac gcagcgcggt tacgagacct gggtcgagat gaccaacgag ggcggcggcc
     7921 tggttggcaa gccggtgaag atcatcgtca aggacgacgc caccacacaa gacaccgccg
     7981 tcagcaatta caacaacctg atcgccaagg ataaggtcga tctgctgttg ggcagccaat
     8041 cgtcgctgct gaacatcccc gcatcggcgg tcgcggaaaa gaacaagatg ctgttcgtct
     8101 gcccgtcctg cgcctcgccc gacatgttct cgcgcgggtt ctcctacatc ttcttctccc
     8161 agcaggccgt ggccagcgac caggccaagg tgttcgccca ctggatcgcc aacctgccgc
     8221 ccgaccagcg gccgaagacc gcggcctatc ccgcacttga cgacccgttc gccggccccg
     8281 cggtggacgg cgccgagaag attctctcgg cggccggtat cagaaccgtc tacaaggacc
     8341 aatatccaac caccacgaag aatttcgact cggtggtcaa cgccatgaag gacgcaggcg
     8401 ccgaaatggt ggtgcagggt gcgcaattcg aggacggcgt caacatgatc cgtgcgatga
     8461 accgtgccgg ctacaagccg tcgatcgtct tccagagcag ttcgcccacc tacggacagc
     8521 agtacctcga cggtgtggga gcgcaaaacg ccgagggcgt gttctcatcg gccagctaca
     8581 gcccgatcgc taagaccgcc gacaacgccg agttcgtgaa gcgctacgag cagaagtacg
     8641 gcaccacgcc gcccgaggat gccgccgacg gtttcgccgc ggcccaggtg ctcgccgccg
     8701 ccgtcaacgc ggtcggctcc tacgacgacc aggccaagct cgcagactgg ttgcgcgcca
     8761 acatagttga gaccgtcctc ggaccgttga gctggaacgc cgatggcagc cccaagggtg
     8821 agttcctagt cggccaatgg cagggcgggg tctcccaggt tgttctgccg accgatgtcg
     8881 ccaccaccga caagatcctg cgctggcagg gcggcgtcat ctaggcaccg gatcgacact
     8941 aggagcccct acgatggtct cgctcgttca aacgctcatt ctcggcctgc tggtcggcgc
     9001 gctgtatgcg ctgatggcct ccggcctgac cctgatcttc ggggtcatgc gggtgatcaa
     9061 cttggcccac ggcgcgttcg cgatcctcgc cgcgttcctg acattcacgt tgtggcgcaa
     9121 cctcggtatg gatccgatcc tgtccatccc cgtcgtgatg gccgtgatgt tcgcgatcgg
     9181 gtggctggta tacgtcctgg tgatccggca cgtccgcggc gcccacgtga gcatgacggt
     9241 gctcgccacc tttggcatcg cgctggtgct cgagggagcg atgggcttca tctggggtaa
     9301 cacctccacc gcggtgcgga cctcctatac agacgcgtcg ttgtcactcg ggccgctgta
     9361 cgtgccgcag gcgatgctga tcaccgcggc aatcgcggcg ttggtcatcg gcggcctgta
     9421 cctcctgctg aactacacct ggaccggccg ggccatccgc gccgcgtcga cgaacgaagg
     9481 gggagcgctg ctcgtcggtg tcagcgtggc gaccgtcgcc gcgctcacct tcgccatcgg
     9541 tgtggccacc ctcggtgccg gcggggcgat gctgtcgacg atctatccct tcctgcctgg
     9601 cacgcactac cagtggatcg cccggttgct ggccatcgtg gtgctcggcg gcctcggcag
     9661 ccttcccggc gcgattctcg gagccctggt catcggggtg ggggagatgg ccgccaccaa
     9721 ctatctcggg gcagcgtggc ccgtcgcggt gcctttcctg gtcatcatcg tcgtgctgat
     9781 cacccgtccc cagggcatac tcggcacccg gctcagagag gacgcggtcg catgaccacg
     9841 atttcggggg cctcccggct gctcgatccg cgcatgcaga agctcatcac ccgcggcctt
     9901 gtgctcgtcg cggcggtcgt gatcgtgctt ttcccattgt cccccagcgc atctgcgcag
     9961 aacacggtga tcctggcgtt ggtgttggcc atcggggcca gcggcctgaa catcatcacc
    10021 ggttacaccg ggtacctctc actgagccag ggcgcgttca ttggcatcgg cgcctacgtg
    10081 ggcgcgatcc tggccacccg gtttccggac gtgcagccgc tgctatgggc gcccatcgcc
    10141 ggcgtggtcg ccgcgttgat cgcgatgctg ctcggcctgg tgacctatcg atcgcggggc
    10201 ccggccttcg tcatcatcac cgtggccttc ctatttctcg tgcaattcgt cgcgatcgaa
    10261 tgggtgcccg tcacgaacgg cacggccggg ctgacgctgc cccttccgga ttggcccgac
    10321 ccgtggggca attggccctt ccacctggct ctggtggtgc tgttggcgtt gtcactgacg
    10381 atgacctggt ggatccggcg cactaaattc ggtatgggcc tcatcgcgat tcgcgaggat
    10441 gaaggcaagg ccggcacgat cggcgtcaac gcacccgttt acaagctcct cgcattcggg
    10501 gccagcgcac tgttcgtcgg tatggcgggt tctgtgtacg gctactacct gtccttcgtc
    10561 gatccgatcg gcatgttctc gatcatcacc agcgcgctga tcgtgttgtc ggtgatcctg
    10621 ggcggccgcg ggacgctgtt cggaccggtg ctgggcgcgt tcatcatcca gccggtcaac
    10681 aacttcgcca atgacacgtt cggcggaggc aacgcgcgcc tggtgatctt cggtggcttg
    10741 ctcgtactgc tggtcattct gttgccgaaa ggcatcctgc caaccgccgc cgcactgctg
    10801 gagaagacca gatccaaagg caccgttggc ctttccggtg cccggctgaa cccgctggac
    10861 cggcctctcg ctcctgcgga ggcccgcccg cccgcgccga ccggacgcac cctgctcgaa
    10921 gtccgtggtc tgcacaagag cttcggcggc aaccacgcgg tgaacgactg cagcttcacc
    10981 gtgccggagg gttcgatcac cgcgctgatc ggaccgaacg gctctggcaa gacgacggtg
    11041 ttcaacctga tctcgggcac gatgacgccc gaccgcggcg aaatcctgtt gaacggtgaa
    11101 cgcatcgaac gactttcgcc gtggtcgcgg gcgcaccgcg ggatcggccg gacctttcag
    11161 atcacccggc tgttccggga gatgaccgtg ctcgagaacg tcgtagcccc gctgcgagac
    11221 ttctcggtgc gccagctcgg gctgggtgcg gtcagcggtt cggaggccgc tcgcgccgaa
    11281 gagctgctcg agttcgttgg gatgtcggca taccgggatg ctcgcgccgg ggcgctgtcc
    11341 tacggtcagc agaagctggt cgagctcgcc caggtcctca tgctggaccc gaagttgatc
    11401 ctgctcgacg agcctgcagg cggcatcaac ccgacgctga tcgagcggat gggcgacctg
    11461 atccgcgcgc ttaacgctca ggggaccacg ttcctgttgg tcgagcacaa catgccgttc
    11521 gtcgtcggct tgtgcgatcc gatccgggtg ctggcccgcg gcgcggtcat cgcgcagggc
    11581 tcgcctgagc agatccagaa ggacccgctg gtgctcgacg cctatttggg tgacgactac
    11641 ctgctggagg cgccgtcgcg gtccgtgaat atccctgtgg tggagaggaa tccagcatga
    11701 tcaggctcga aggcgtagtc gccggttacg gcggcggcaa tgtcctgcag ggtgtcgacc
    11761 tggaagtgaa tacaggcgaa ctcggctgca tcgtcgggcc caacggggcg ggcaagtcca
    11821 ccgtgctgcg cgcggtcagc gggctgctca aaccaagcgc gggacgtatc ctgctcgatg
    11881 gcgaagacat cacaggccgg acgcccgacg ccgttctggc gcgcggtgtc acccaggtgc
    11941 cgcagagcaa cgggctgttc gcgaccctga ccgtgcggga aaacatcctg atgggcgcgt
    12001 acgtgatccg caaacagcgg gcgctggtgc gtcagcgcta cgacgaagtg ctcgacatgt
    12061 tccccctcgt ccagaacaag accggggtcc gatgcggcaa cctctccggc ggccagcgcc
    12121 gcatggtgga attcgcccgc tcactgatgc tcgacccgaa actcgttctt cttgacgaac
    12181 cgtcgctagg tctggatccc aagtcgctca ccgtgataga cgaggcggtc gccatcatgc
    12241 gggccaaagg caaggccgtg ctgatggtcg agcagaacgt gcgcttcggg ttgcggatgg
    12301 ccaccagcgg catcgtcatg gaaagtggcc gggtgttgct caccggaccc gcccagtcgg
    12361 tgctgtcgaa tccagagatc gccgacctct acttcggcgg ggcggtacac gcacccgtcc
    12421 aatccgacac cgccgccaac ca
//
LOCUS       DASRJA010000141         4847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1902985,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000141 DASRJA010000000
VERSION     DASRJA010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902985"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..735)
                     /locus_tag="VFC01_06515"
     CDS             complement(202..735)
                     /locus_tag="VFC01_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79327.1"
                     /translation="MSAPTESRILADTGGLVVTDDGRRVLVFDRCTGALTVLAFVLGV
                     ITLVVAGFGAVTLVAGVPSSTLGAAFLAVGLAIGAVTYLVVRKFRRRRSQPLHECRPV
                     AVIDRKLGLFSYGGGAVVPLDQVQFGRRFQIGSSSPKLVAVTPGGTHVLKRGNPFDGG
                     IGNVDGLLTSVAKNAVI"
     gene            complement(876..2012)
                     /locus_tag="VFC01_06520"
     CDS             complement(876..2012)
                     /locus_tag="VFC01_06520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZN79328.1"
                     /translation="MTSPPVKPPVGGQITPLLASTLSIPRWFTGTDDRAHLVYELLLT
                     NAVPAPVTLNAVEVHDAESGAMLTRLTGDSLDAATSLAASAETPTVALPPSSVGIVWM
                     DISLPDDNIPAAITHRITIDPTPDVPLPPEELTFAGMATEVERRPAVVLGPPLAGARW
                     AALGGCCDGPHRRALYPIDGRWYLAQRYAIDFNQLDAENRPGTGDPTLPTSFPTFGQP
                     VYAVADGTVAIADDGNPDLRVGEGREEPTPANAGGNRVVIDIGDGRFAVYAHLRQGSI
                     SVRPGDHVGRGRQIANVGSSGTTGGPHLHFQVTDRPSVVFADGMPYVFDSFELTGRTP
                     PLAEVLPYYDTLQAIPISTDRTGARRDEMPLGRDVVTFPAAKGR"
     gene            complement(2248..2940)
                     /gene="urtE"
                     /locus_tag="VFC01_06525"
     CDS             complement(2248..2940)
                     /gene="urtE"
                     /locus_tag="VFC01_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HZN79329.1"
                     /translation="MLQLVDVHTGYGRSEVIHGVSLEVPSDGVAAVMGHNGAGKTTLL
                     RVAVGLLKCTAGKVVFDGDDITKLRPSARVARGLAYVPQGQQSFGQLTTAENLQVVAD
                     GRKKGKLLIDEQLDLFPALKELLTRRAGLLSGGQRQQLAIARALITSPKCLILDEPTE
                     GIQPSVVAEIEAAITELTKRGDLGVLLVEQHIGFALESSQRYYILESGRITSSGTGGS
                     ASEADVRAAMAI"
     gene            complement(2940..3764)
                     /gene="urtD"
                     /locus_tag="VFC01_06530"
     CDS             complement(2940..3764)
                     /gene="urtD"
                     /locus_tag="VFC01_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HZN79330.1"
                     /translation="MTTAELEPVAGGNVGMGTEYLEVRGLTVDFDGFKAVSDVDLTLF
                     QGDLRFLIGPNGAGKTTVIDAITGLVPATGSVNKSGAELLGKKVHQIARRGVGRTFQT
                     ASVFEQLTVLQNLDIAAGAGRSPWTLLRRRRGILPAIEEALETTGLTELADKPAGVLA
                     HGQKQWLEIGMLLVQNADVLLLDEPVAGMSHEEREETGNLLRRIGDERTVVVVEHDMD
                     FMRAFATSVTVLARGQVIAEGSVAEVQANPKVQEVYLGTAAAGAEGIPDELIEESS"
     gene            complement(3761..>4847)
                     /gene="urtC"
                     /locus_tag="VFC01_06535"
     CDS             complement(3761..>4847)
                     /gene="urtC"
                     /locus_tag="VFC01_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HZN79331.1"
                     /translation="QTWAGFGVAAVLLFAVAPAVLSDFRLSLLGKFLCFAIVAVGIGL
                     AWGRGGMLVLGQGVFFGLGGYMMGMHLKIADAQLRGTSVPDFMQIAGVRALPAYWEPF
                     ASPAFTLLAIVLVPTVIAAVLGLGVFKRRVKGAYFAILSQALAAALAILLVGQTSVGG
                     SNGLNNFRTFFGFALKDPANKRMLYFIAAGVLLVVVAVVRQLMYSRYGELLVAVRDGE
                     ERVRFLGYDPANIKVVAYTVAAFFASIAGALFAPIVGFIAPSQVGILPSIVFLIGVAI
                     GGRTTLLGPVLGAIGVAWAQSLFSERFPSEWTYAQGLLFIVVVGFFPAGLAGLGALFK
                     RIRRTKDAPVVLDPDPEPEAEKVGAAT"
BASE COUNT          886 a         1598 c         1575 g          788 t
ORIGIN      
        1 acgcggcgga agccgtcgat aaaggtctgg cgggtcaact cggcttgcgg gccgatccat
       61 ttgttcgcat aggcgacggc gccgagatct acggccagga acgcgccgac cgcaccgatg
      121 cccagcaaaa ccgagcgtcg actgagttga gtcacaaatt ggcaaaacgc gccgcggcac
      181 ggattgcttc ggcgttgtgc tctagatcac agcattcttc gctaccgacg tcagcaatcc
      241 gtcgacattg ccgatgccgc cgtcgaatgg gttgccgcgc ttgaggacgt gtgtgccacc
      301 aggcgtcacc gcgacgagtt tgggtgagct cgaaccgatt tggaatcggc gtccaaactg
      361 cacttgatcc aacggaacca ctgctccgcc gccgtaactg aacaacccca gcttgcggtc
      421 gattaccgcc accggccggc attcgtgcaa tggctggctg cggcggcgac gaaacttgcg
      481 caccacgaga taggtcactg ctccgatggc cagaccgaca gccaggaacg ctgcgcccag
      541 cgtgctcgag gggacgcccg cgacgagcgt gaccgcgccg aagccagcga cgacgagagt
      601 gataacgccg agcacgaacg ccaacaccgt cagcgcaccc gtgcagcgat cgaagaccag
      661 gactcgtcgg ccgtcatctg tgacgacgag tccgcccgtg tcggcaagga tgcgtgattc
      721 ggtcggcgcg ctcatcccgg acagcatgcc gaccattggc ggcgagcgtg cgcgtttgca
      781 cggaaacagc ggcgtgtcgt gtgcagacac ggacgctcgc gggcaagtca gatggccatg
      841 gcaggctcac tgccccacgg cgacagccga acgcatcatc tgcccttggc ggcggggaac
      901 gtcacgacgt cacggccgag cggcatctcg tcgcggcgcg cccctgtgcg gtcggtgctg
      961 atgggaatcg cttggagggt gtcgtaatac ggcagcactt cggccagggg tggtgtccga
     1021 ccggtcagct cgaaactgtc aaagacataa ggcatcccgt cagcgaacac gaccgaggga
     1081 cggtcggtga cctggaaatg caggtgcggg ccgcccgtag ttcccgaact gcccacattg
     1141 gcgatctggc ggccgcgacc aacgtggtcc ccgggacgaa ccgatatgct gccctgccgc
     1201 agatgggcgt acacggcgaa gcggccgtcg ccgatgtcga tcaccacgcg gttgccaccg
     1261 gcgtttgcgg gtgtcggctc ctcgcgtccc tcgccgaccc gtagatccgg gttgccgtcg
     1321 tcggcgatgg caaccgtgcc gtcggccacg gcgtacaccg gctgaccgaa tgtgggaaag
     1381 ctcgtcggca gtgtgggatc gcctgtgccc ggccgattct cggcgtcaag ttggttgaag
     1441 tcgatcgcgt accgctgtgc gagataccac ctcccgtcga tcggatacag ggcgcggcgg
     1501 tgtggcccgt cgcagcagcc gccgagggct gcccaacggg cgcctgccag cggggggccg
     1561 agtacgaccg caggccggcg ttcaacttca gtcgccatgc cggcgaacgt caattcctcc
     1621 ggagggagcg gcacatccgg cgtggggtcg atcgtgatgc ggtgggtgat cgctgccggg
     1681 atgttgtcgt cgggaagcga tatgtccatc cagacaatgc cgacggagga gggcggtagg
     1741 gcgacagtgg gagtttcggc cgatgcggca agcgatgtgg ccgcgtccag ggagtcgccg
     1801 gtcagccggg tcaacatggc tcccgactcc gcgtcgtgca cctcgacggc gttcagcgtg
     1861 accggcgcgg ggacggcgtt ggtcagtagt agttcgtaga ccaggtgagc ccggtcatcg
     1921 gtgccggtga accagcgtgg tatcgacaac gtgctcgcga gtaacggtgt gatctggccg
     1981 cccacggggg gcttgaccgg tggcgaagtc atggtcggct gtgacgaaca agcgctgagc
     2041 acaaccgcgc aaacggcgat gaacgagcgc acaactgagg ccgcggcctg accggttctt
     2101 gcaggcccgg attcgcaatc acgctggatc gcccagccga gttcgcgcaa cccacaccac
     2161 ctcggttgtt gacgttcgtg atcatatgca gcggccggac gatgggtgct ggtttcaata
     2221 cgatcgggcg ggcgtctcgc gggcaagtca gatggccatg gcggcgcgga cgtcggcttc
     2281 cgacgccgag cctccggtgc cggacgacgt gatgcggccg gactccagga tgtaataccg
     2341 ctgcgacgac tcgagggcga agccgatgtg ctgctcgacc agcagcacgc ccagatcacc
     2401 gcgcttcgtc aattcggtga tcgcagcttc gatctcggcg accaccgacg gctggatgcc
     2461 ctcggtgggc tcgtcgagga tcaggcattt ggggctggtg atcaacgccc gggcgatcgc
     2521 cagctgttgc cgttggccgc cggacagcag gccggcccgc cgcgtcagca gctccttcaa
     2581 agcgggaaac aggtcgagct gctcatcgat cagcagcttg cctttcttgc ggccgtcggc
     2641 gacgacttga aggttctcgg cggtcgtcaa ctgcccgaag gactgctggc cctgcgggac
     2701 gtacgccaac ccacgcgcca cccgcgcgct gggccgcaat ttggtgatgt cgtcgccatc
     2761 gaaaaccacc ttgcctgcag tgcatttcaa cagccccacg gcgacacgca agagcgtggt
     2821 cttgccggcg ccgttgtgac ccatcaccgc cgccacgccg tcggacggca cttcgaggct
     2881 gacgccgtgg atcacctcgg agcggccgta gccggtgtgg acgtcgacaa gctgcagcat
     2941 caggaactct cctcgatgag ttcgtccggt attccttcag cgccggcggc ggcggtaccg
     3001 aggtagacct cctgcacctt cgggttggcc tgcacctcgg cgaccgaacc ctcggcgatc
     3061 acctgccccc gtgccaacac agttaccgac gtcgcgaacg cccgcatgaa gtccatgtca
     3121 tgttcgacga cgacgacggt gcgttcatcg ccgatgcggc gcaacaggtt tccggtttct
     3181 tcgcgctcct cgtgactcat gcccgcgacc ggctcgtcca gcaacagcac gtcggcgttc
     3241 tgcaccagga gcatgccgat ctccagccac tgtttctggc cgtgcgccag cacaccggcc
     3301 ggcttgtcgg ccagctccgt cagccctgtg gtctccagag cttcctcgat cgccggcagt
     3361 atgccccggc gcctgcgcaa tagcgtccac ggtgagcggc ccgcccccgc ggcgatatcc
     3421 aggttctgca gcacggtcag ctgctcgaaa acgcttgcag tctggaatgt ccggcccacg
     3481 ccgcgccgcg cgatctggtg caccttcttg ccgagtagct cggcaccgga cttgttcacc
     3541 gatcccgtcg cgggcaccag tccggtgatc gcgtcgatga cggtcgtctt gcctgcgccg
     3601 ttgggtccga tcaggaatcg caggtcgccc tgaaacagcg tcagatcgac gtcgctgacc
     3661 gccttgaaac cgtcgaaatc aaccgtcaga ccgcgcactt cgagatattc ggtgcccatg
     3721 ccaacattgc caccggcgac cggttccagc tcagcagtgg tcatgttgcg gctccgacct
     3781 tttcggcctc gggttcggga tcgggatcga gtacgacggg cgcgtccttg gtgcgtcgga
     3841 tccgcttgaa caacgcaccc aaaccagcga gcccggcggg gaagaagcca acgacgacga
     3901 tgaacagcag accttgcgcg taggtccact ccgacggaaa gcgctcggag aaaaggcttt
     3961 gcgcccatgc gaccccgatg gcaccgagca ccggcccgag cagcgtggtg cgcccgccga
     4021 tggcgacgcc gatgaggaac acgatcgacg gcaggatgcc gacctgtgat ggtgcgatga
     4081 agccgacgat cggtgcgaac agtgctccgg cgatgctcgc gaagaacgcg gcgacggtat
     4141 atgccaccac cttgatgttg gcggggtcat agcctaggaa tcgcacgcgt tcctcgccgt
     4201 cgcgcaccgc gaccagcagc tcgccgtagc ggctgtacat cagctggcgc accactgcga
     4261 ccacgacgag caataccccc gcggcgatga agtacagcat ccgcttgttc gccgggtctt
     4321 ttagcgcgaa cccgaagaac gtgcggaagt tgttgagccc gttgctgcct ccgacactgg
     4381 tctgtccgac cagcagaatg gccagggccg ccgccagcgc ctgggacagt atcgcaaagt
     4441 aggcgccctt gacccgacgc ttgaacacac ccaaaccaag tacggccgcg attaccgtcg
     4501 gcaccagcac gatcgccagc agcgtgaaag cgggagaggc gaatggctcc cagtacgcgg
     4561 gcaacgcgcg taccccggcg atctgcatga agtccggcac gctggtgccg cgcaactgcg
     4621 cgtcggcgat cttcaggtgc atacccatca tgtagccgcc gagcccgaag aagacgccct
     4681 ggccaagcac cagcatgccg ccgcggcccc acgccagccc aatgccgacg gcgacaatcg
     4741 cgaagcacaa gaacttcccg agcaggctga gccggaagtc ggagagcacg gcgggtgcca
     4801 ccgcgaacag cagcaccgcc gccacgccga atcccgccca ggtctgc
//
LOCUS       DASRJA010000142         8272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2711038,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000142 DASRJA010000000
VERSION     DASRJA010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2711038"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFC01_06540"
     CDS             <1..248
                     /locus_tag="VFC01_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN79332.1"
                     /translation="GGWWVLGVTDATMADCLRTIPMSRSDTGAVTLAALGDTGLNVSL
                     VTALADVDSVDDIDVVRHECAPESRFVRVTRGVSHAR"
     gene            238..867
                     /locus_tag="VFC01_06545"
     CDS             238..867
                     /locus_tag="VFC01_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN79333.1"
                     /translation="MLGNLYDRALAGERCWLRHDNGRVDRLPVRSWLGGRHADARFDH
                     AVVGLCNGPTIDLGCGPGRLVAHLVQRGVPALGVDQSATAIALARRSGAPALRRDVFD
                     PLPGTGRWQTVLLADGNVGLGGDPRRILRRACELLRYGGRCVAEFDSAIKGIHAGWVR
                     LESSRRIGPWFPWASVGIDCAARLADDVGLAVAVVHPIGRRVVASLVAT"
     gene            864..1964
                     /locus_tag="VFC01_06550"
     CDS             864..1964
                     /locus_tag="VFC01_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN79334.1"
                     /translation="MKAATLRGTSITARVGLALGVAVAVCFVTGLISHFIQHPQPWFF
                     WPTRPVWLYRVTQGLHVISGIAAIPLIIVKLWSVWPKLFERPVIGGLVRQIERLSILV
                     LVGSMLFQLSTGLLNIAQWYAFKFFFTTSHYAMAYVAAGAVLVHIGVKLPILRRALGE
                     PVDDTPASVGPTRRTVLRATWLAVALATVVTAGQTVPLLRKVSVLAPRSGQGPQGVPI
                     NRSAIAAGVLGSAQSPDYRLTVTNGPTSRAFSLADLQAMPQATHRLPIACVEGWSADA
                     EWTGVVVGDLVAAVGGSRDSDVRMISLEPPGPYSRTVLPARHVRDGKTLIALKLNGQT
                     LDLDHGYPCRLIAPTRPGVLQTKWLSRIEVVP"
     gene            1961..2419
                     /locus_tag="VFC01_06555"
     CDS             1961..2419
                     /locus_tag="VFC01_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79335.1"
                     /translation="MRIALVLLGVVLGAYGAVLLWENPPVIITRIVVWALVGVVLNDM
                     VFAPVCVALGFAGRRLIPGKWWPPVAVATFLSVVLVFLAIPVYGKPGLRPDNMTVLDR
                     NYPLGLWISLGIVWACVPLYYLAMRRLPVRQDEVVEQESTDDVERQPPAV"
     gene            complement(2345..3700)
                     /locus_tag="VFC01_06560"
     CDS             complement(2345..3700)
                     /locus_tag="VFC01_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79336.1"
                     /translation="MRIGRREAVAVTVGVVLVVAAFAVPHLHLGIVTPLIHASPARLY
                     SFADTAPIFGWWKAHVGWGTVPAIVIGIAAVVWGQAVALRLPWRALPWITWATACTWA
                     FSLAMIDGWYRGFAHRLEARHEYLRQVPTVTDIPEAVRTFASRIPDFQPNSWITHVSG
                     HPPGALLTFVWLDRIGLGGGAWAGLLCLLVGSSAAAAIVVAVRALADENTARLAAPFV
                     AVAPTAIWVAVSADGYFAGVTAWGITLLALAARRTVRWPVVAAAGAGLLLGWGIFLNY
                     GLVLMALPAVAVLLAAVDSRAALSALVPAVIAAMAVVGVFAIAGFWWFDGYTLVQERY
                     WQGIANDRPFQYWSWANFASVVCAIGLGSVAGVGCAFDVAAIRRRPGLHLVVLGALLA
                     IVFADLSMLSKAETERIWLPFAVWLTAAAALLPPRSHRWWLALNVVGALLLNHFILTN
                     W"
     gene            3831..4658
                     /locus_tag="VFC01_06565"
     CDS             3831..4658
                     /locus_tag="VFC01_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN79337.1"
                     /translation="MNTSPFCSVELAERIERAETQMIITATEAARRRLGATAFMMPIA
                     GGAACYAEDGSPLNKIVGLGFGGVPSGDALDEIERAFAERAAPTHIELSNLADPAIGA
                     DLTGRGYHLVSFENVLGRALDGEKGTVAPSGVEVRLSDDREAWLDVVVDGFSDPDVEG
                     VPSHENFPRAIIENAIGDIEQAGATPYLAFRDGVVAGGASMRMSDGVAQLTGAATAPT
                     HRRHGVQTALLSTRLADAARAGCDIAVVTTQPGSKSQQNVQRRGFHLLYTRAVLVKS"
     gene            4697..5293
                     /locus_tag="VFC01_06570"
     CDS             4697..5293
                     /locus_tag="VFC01_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN79338.1"
                     /translation="MPRPRTFDESDVVAAARDEFWSRGYTATSVDDLTSATGLGKGSL
                     YGAFGDKHGLFVRALEDYIGTALDGVRAQLRDPKYSAYDRLVRHIRAQVKAIAADKSR
                     RGCMIAKSAAELGATDDTVERAVDRAYAVWTAELVDSIKEAQRDGAIDKKQNAHALAT
                     TLLAFMRGQEALHKGGAKPTQIRAAAEQMIALIPTGSK"
     gene            5366..6127
                     /locus_tag="VFC01_06575"
     CDS             5366..6127
                     /locus_tag="VFC01_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN79339.1"
                     /translation="MALLKGKTAVITGGSTGIGLATAKRFVEEGAYVFISGRRQTELD
                     AAVKELGDNATGVQGDVAKAEDLDRLYAAVADSGRRIDVVFANAAVIDVARIGDITDD
                     HLDYQLDVDFKGVVFTVQKALPLLNDGGSIILNSSNTNAKGTEGIGVYAAIKAALRSF
                     ARTWASELRHRKIRVNVVSPGATYTPGINTLAGMLIPGPNAEEQFENLQRSIVPMARY
                     GTAEEVANAVLFLASDLSSFTTGADIPVDGGINQI"
     gene            complement(6124..6738)
                     /locus_tag="VFC01_06580"
     CDS             complement(6124..6738)
                     /locus_tag="VFC01_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZN79340.1"
                     /translation="MRREVNTVEELRAIVGEPDRYVANKVKDQLSAIHRDWLAHSPLG
                     FIATTDAQGRVDVSPKGDPPGFVHVIDETTIAIPERPGNKRVDGYQNVLQQPHVGMLF
                     LIPGRGDTLRINGTARILADADYFDAMTVKGKRPILALEIDVEEVFFHCAKAFLRSDT
                     WDPSTWNPTAVPSVAKLAKAIRYDWSQAELDARYSEENMRKVLY"
     gene            6798..>8272
                     /gene="glgP"
                     /locus_tag="VFC01_06585"
     CDS             6798..>8272
                     /gene="glgP"
                     /locus_tag="VFC01_06585"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016891395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HZN79341.1"
                     /translation="MTDVVQNPDTTNERSRTGLSADALRRGIIDHLRYSIGRPAAALR
                     PEHYYRATALAVRDRMQDNRVASTQTSLDLGRKVTCYLSAEFLMGPQLGANLLNLGIE
                     DAVRAALAELGQDLDDVLACEEEPGLGNGGLGRLAACYLDSLATLERPAIGYGIRYEF
                     GIFDQEIHDGWQVEQTDNWLDNGNPWEIAKPDVNYRVNWGGYAEHYTDDAGHDRVRWV
                     PGRVLKGVAYDTPIQGYGVNTCNVLTLWSARAVKSFALEAFNTGDYYKAVEDEVNSET
                     VTKVLYPNDEPEAGKRLRLLQQYFFVSCSLQHVLHIMDDLADASVKDLPQRFALQLND
                     THPSIGVAELMRLLVDERHIDWAEAWDITEATFGYTNHTLLPEALETWPLAMFAESLP
                     RHLEIIYEINRRFLDEVRAKFPGDEARLRRMSLIGEDGGKSVRMAHLAAVGSHAINGV
                     AALHSELLKDSVLKDFYELWPERFSNKTNGVTPRRFLALANP"
BASE COUNT         1426 a         2709 c         2777 g         1360 t
ORIGIN      
        1 acggcggatg gtgggtgctc ggcgtcaccg atgccacgat ggccgattgc ctgcgcacga
       61 ttccgatgtc gcgatccgac accggggctg taacgcttgc ggcacttggc gacactgggt
      121 taaacgtgag cctggttacc gcgctggcgg atgtggacag cgtcgacgac atcgacgtgg
      181 ttcggcacga atgcgcgccg gagagtcggt tcgtgcgggt cacccggggt gtgtctcatg
      241 ctcggtaacc tctacgacag ggcgctcgcc ggcgaacgct gctggctccg tcacgacaac
      301 ggccgggtcg accggttgcc ggtgcgcagc tggctcggtg gccgccacgc cgacgcgcgg
      361 ttcgaccacg ccgttgttgg gctgtgtaac ggcccgacga tcgacttggg ttgcggcccc
      421 ggacgtttgg tggcccacct ggtccagcgg ggagtgcctg cgttgggcgt cgaccagtcc
      481 gccaccgcca tcgcactagc gcgacgcagc ggcgccccgg ccctgcgccg cgacgtgttc
      541 gatccgctgc cggggaccgg ccgctggcag acggtgctgc tggccgacgg caatgtcggt
      601 ctcggcggtg acccgcggcg gattctgcgg cgcgcgtgcg aactactgcg ctacggagga
      661 cgctgtgtgg ccgaattcga ctcggccatc aaggggatcc atgcaggttg ggtccgactc
      721 gaatcgtcgc gccgtatcgg cccgtggttc ccatgggctt ccgttggcat cgattgcgcg
      781 gcacggttgg ccgacgacgt tggccttgcg gtcgcggtgg tccacccgat cggccgtcgc
      841 gtggtggcca gcctggtcgc gacatgaagg cggccacctt gcgcggcacg tcgattaccg
      901 cacgcgtcgg tctcgccttg ggtgtcgcag tggcggtgtg tttcgtcacc gggctgatca
      961 gtcacttcat ccagcacccg cagccatggt tcttctggcc cacccggccg gtgtggctgt
     1021 accgcgtcac ccagggcctg catgtcatct cggggattgc ggccattccg ctgatcatcg
     1081 tcaagctgtg gtcggtgtgg ccgaagctgt tcgagcggcc cgttatcggt gggctggtcc
     1141 ggcagatcga gcggttgtcg attctggtgc tggtcgggtc catgctgttt cagctgtcga
     1201 ccgggttgct gaacatcgcg cagtggtacg cgttcaagtt cttcttcacc accagtcact
     1261 acgcgatggc gtacgtggcc gcaggggcag tactggtgca catcggcgtg aagctgccga
     1321 tcctccgccg cgcgctgggg gagccggtcg atgacacgcc cgcttctgtg gggccgacgc
     1381 ggcgcacagt gctgcgcgct acatggctcg cggtggcgct tgccacagtc gtgacggcgg
     1441 ggcagacggt gccgctactg cgcaaggtgt cggtgctcgc gccgcgttcg ggccaagggc
     1501 cgcaaggcgt tccgatcaac cggtcggcga tcgctgcggg tgtgctgggc agcgcgcagt
     1561 caccggacta ccggttgacg gtgacgaacg gaccgacttc gcgggcattc agcctcgccg
     1621 acttgcaggc gatgccgcag gccacacaca ggttaccgat tgcgtgtgtc gaagggtgga
     1681 gcgccgacgc ggagtggacc ggggtggtgg ttggcgacct cgtcgccgcg gtcggtggct
     1741 cacgcgactc ggacgtccgg atgatctcgc tggaaccgcc agggccgtac tcgcggacgg
     1801 tgctaccggc gcggcatgtc agggacggca agacattgat cgcgctgaaa ctcaacggcc
     1861 agacgctgga cctggaccac gggtatccct gccggctgat cgcgccgacc aggcccggtg
     1921 tcctgcagac gaaatggctc tcccgtatcg aggtcgtgcc atgagaatcg cgctcgtcct
     1981 actcggagtg gtgctcggtg cctatggcgc agtgctgctg tgggagaacc cgccggtgat
     2041 catcacgcga atagtggtgt gggcgttggt cggcgtcgtg ctgaacgaca tggtgttcgc
     2101 gccagtgtgc gtggcgctgg gcttcgccgg ccgcaggctc ataccgggga agtggtggcc
     2161 accagtcgcg gtggcgacgt tcttgagcgt cgtgctggtg ttcctggcga tcccggtgta
     2221 cggcaagccg ggcctgcgcc cggacaatat gacggtactg gaccgcaatt acccacttgg
     2281 cctgtggatt tcgctcggaa tcgtgtgggc ctgtgtgccg ttgtattacc tcgcgatgcg
     2341 gcggctacca gttcgtcagg atgaagtggt tgagcaggag agcaccgacg acgttgagcg
     2401 ccagccacca gcggtgtgac cgcggtggca acagcgccgc ggccgcggtg agccataccg
     2461 caaacggcag ccagatgcgt tcggtctcgg ccttgctgag catggacagg tcggcgaaca
     2521 cgatcgccag cagcgcgccg agcaccacga ggtgcaggcc ggggcgtcgc ctgatcgccg
     2581 cgacgtcgaa tgcgcagccg acaccggcga cgctgcccag cccgatcgca cacaccaccg
     2641 atgcgaagtt cgcccacgac cagtactgaa acggccggtc gttggcgatg ccctgccaat
     2701 agcgttcctg caccagcgtg tagccgtcga accaccagaa ccccgcgatt gcgaacacgc
     2761 caacgaccgc catggcggca atcaccgcgg gaaccaacgc acttagggcg gcccgcgagt
     2821 ccaccgccgc gagcagcact gccaccgcag gcagggccat cagcacaagg ccgtagttca
     2881 ggaagatgcc ccagcccagc aggagcccgg cacctgcagc ggccacgacc ggccagcgca
     2941 ctgtgcgccg agcagccaac gccaacagcg tgatacccca cgcggtcacg ccggcgaagt
     3001 aaccgtccgc agacaccgcg acccaaatgg ccgtcggcgc gaccgcgacg aacggcgccg
     3061 ccagccgagc agtgttctcg tccgccaggg cgcgcaccgc caccacgatc gcggccgcgg
     3121 cgctcgaccc gacaagcagg cacagcagtc ccgcccatgc gccgccgccc aggccgatgc
     3181 ggtcaagcca gacgaacgtc agcagcgcgc ccggcgggtg ccccgacacg tgggtgatcc
     3241 acgaattcgg ttggaaatca gggattctgc tggcgaacgt gcggaccgcc tcgggaatgt
     3301 ctgtgacggt tggtacttgg cgcaagtact cgtgccgcgc ttcgaggcga tgcgcgaagc
     3361 cgcggtacca gccgtcgatc atcgccaggg agaacgccca cgtgcacgcg gtggcccagg
     3421 tgatccacgg cagtgcccgc cacgggaggc gaagcgcgac cgcctgcccc cagaccacgg
     3481 cggcgatgcc gatgacgatc gcaggcacgg tgccccagcc gacgtgtgct ttccaccacc
     3541 cgaagatcgg cgcggtgtcg gcgaagctgt agagacgcgc gggggaggcg tggatcagcg
     3601 gggtgacgat gccgaggtgc agatgcggga cggcgaatgc cgcgacgacc aggacgacgc
     3661 cgacggtgac cgccaccgcc tccctgcgac cgatccgcac gtccgccagc ctattgcgct
     3721 gccaggcgga cccgtctgac gaatcggtga cgcatcaggc gtggtgctca tcggtgtccg
     3781 ctgtggaccc ccagcgattc cctcagcgcc cgcactgatt cgatgaggat atgaacacct
     3841 cgccgttctg cagcgtcgag ttggcagagc gcatcgaacg tgccgaaacg caaatgatca
     3901 ttacggcgac cgaggccgca cgacggcgct tgggtgcgac ggcgttcatg atgcccatcg
     3961 caggcggcgc ggcgtgctac gccgaggacg gctcgccgct gaacaagatc gtgggcctcg
     4021 gcttcggtgg tgtgccgagc ggcgacgctc tcgacgagat cgagcgggcg ttcgcagagc
     4081 gtgcggcgcc cacgcatatc gagctgtcca accttgcgga tcccgcgatc ggtgcggact
     4141 tgacgggccg tggctatcac ttggtgtcgt tcgagaacgt gctgggacgc gccctggacg
     4201 gcgagaaggg gacggtggcc ccgtccgggg tcgaggttcg gttgagcgac gatcgcgagg
     4261 cctggctcga tgtcgtcgtc gacggcttca gcgatccgga cgtcgagggg gtgccctcac
     4321 acgagaattt cccgcgcgcg atcatcgaga acgcaatcgg ggatatcgag caggcaggcg
     4381 ccacaccgta tctcgcattc cgcgacggcg tcgtcgccgg tggcgcgagc atgcgcatgt
     4441 ctgacggcgt agcgcagctg acgggtgcgg cgacggcgcc gacgcatcgc cgtcatggcg
     4501 tacagacggc gctgttgtcg acgaggctgg ccgacgctgc gcgcgcaggc tgtgacatcg
     4561 ccgtcgtcac cacccagccc ggatcgaaat cgcagcagaa cgtgcaacgc cgtggtttcc
     4621 acctgctcta cacccgcgca gtgctcgtca agagctgatc cgtaccgttc gttccataac
     4681 ggcgtagcct ggatgaatgc cccgtccgcg cacgttcgat gaaagcgatg tggtcgcggc
     4741 cgcgcgcgac gagttttggt cgcgcggata caccgcgacg tcggtcgacg acctgacatc
     4801 cgccaccggc ctgggcaagg gcagcctgta cggggcgttc ggcgacaagc atggactctt
     4861 tgtgcgtgcg ctcgaggatt acatcggcac cgccctcgac ggcgtgcgcg cccagctccg
     4921 cgaccccaag tacagcgcct acgaccggct ggtccgccac attcgcgcgc aagtcaaggc
     4981 catcgccgcc gacaagtcac gccgcggctg catgatcgcc aagagcgccg ccgagctggg
     5041 cgccaccgat gacacggtgg agcgtgccgt cgaccgcgcg tacgcggtgt ggacagccga
     5101 gctcgtcgac agcatcaagg aagcgcagcg cgacggtgcc atcgacaaga agcagaatgc
     5161 acacgccctg gccacgacgc tgttggcctt catgcgcgga caggaggcgc tgcacaaggg
     5221 gggtgccaag cccacgcaga tcagagctgc cgccgaacaa atgatcgcgc tgatccccac
     5281 cggctcgaaa tagcacggaa taggaccgcc ggtttatgcg tcgtagttct tgaccgaata
     5341 gtccagaact atcgaaggca ggctcgtggc acttctcaag ggcaagacag cggtaatcac
     5401 aggcggcagc actggcatcg gcttggcaac ggccaagagg ttcgtcgaag agggcgcgta
     5461 cgtcttcatc agcggtcgcc gccagaccga actcgatgcg gcggtcaagg aactcggtga
     5521 caacgccacc ggtgtccagg gtgacgtggc gaaggccgag gatctcgacc ggctgtacgc
     5581 cgcggtcgcc gacagcgggc gccgcatcga cgtggtcttc gcgaacgcgg ccgtgatcga
     5641 cgtcgcgcgg atcggcgaca tcaccgacga tcacctggac tatcaactcg acgtcgactt
     5701 caagggcgtc gtgttcaccg tgcaaaaggc gctgccgctg ctgaacgacg gcggttcgat
     5761 cattctcaac tcctcgaaca cgaacgccaa gggcacagaa ggcatcgggg tgtacgcggc
     5821 catcaaggcc gcgctgcggt cgttcgcccg cacctgggca agtgagttgc ggcaccgcaa
     5881 gattcgcgtc aacgtggtca gccccggagc tacctatacg cctggcatca acaccctcgc
     5941 cggcatgctc atcccagggc cgaatgccga ggagcagttc gagaacctgc agcgcagcat
     6001 cgtgccaatg gcgcgttacg gcacagcaga agaggtcgcc aacgccgtgc tgttcctggc
     6061 gtccgacctg agcagcttca cgaccggtgc cgacatcccg gtcgacggcg gcatcaacca
     6121 gatctagtac aacaccttgc gcatgttctc ttcgctgtag cgcgcgtcga gctcggcctg
     6181 gctccagtcg taccggatcg ccttggccag cttcgcgacg ctcggcaccg cggtgggatt
     6241 ccacgtcgac ggatcccacg tgtccgagcg cagaaacgcc ttagcacaat ggaagaacac
     6301 ctcttcgacg tcgatctcga gcgcgaggat gggccgctta cccttgaccg tcatcgcgtc
     6361 gaagtagtcg gcgtcggcga ggatccgcgc ggttccgttg atccgcaacg tatctccgcg
     6421 accgggaatg aggaacagca tgccgacatg cggctgctgc agcacgttct ggtagccgtc
     6481 cacgcgcttg ttgcccggcc gctcgggaat cgcgatcgtg gtctcgtcga tgacgtggac
     6541 gaagcccggc gggtcgccct tgggtgagac gtcgacgcgg ccctgcgcat cggtcgtcgc
     6601 gatgaagccc agcggggagt gagccagcca gtcgcggtgg atcgccgaca gctggtcctt
     6661 caccttgttc gcgacgtagc ggtcgggctc accgacgatc gcacggagct cctcgacggt
     6721 gttcacttct cgacgcattg caacatcatc ctccttacgt tcattttgca gttgcccacg
     6781 tttgtcacac tccgccgatg accgacgtcg tccagaaccc cgacaccacc aacgagcgca
     6841 gccgcaccgg gctgtccgcc gacgcgttgc gccggggaat catcgaccac ctgcgctact
     6901 cgatcggtcg cccagcggcc gccctgcggc ccgagcacta ctaccgggca acggcgctgg
     6961 ccgtgcgcga ccggatgcag gacaaccggg tggcatcgac gcagacgtcg ctcgacctcg
     7021 gcaggaaggt gacgtgctac ctgtcggccg aattcctgat gggcccgcag cttggcgcca
     7081 acttgctcaa cctgggcatc gaagacgcgg tacgggcggc gctggcggaa ctcggccagg
     7141 acctcgatga cgtgctggca tgcgaggaag agccgggtct gggcaacggc ggcctgggac
     7201 ggctggccgc gtgctatctc gactcgctcg ccacgctcga gcgccccgcc atcggctacg
     7261 gcatccgcta tgagttcggc atcttcgacc aggagatcca cgacggctgg caggtcgaac
     7321 agaccgacaa ctggctcgac aacggcaacc cgtgggagat cgccaagccc gacgtcaact
     7381 accgggtcaa ctggggcggc tacgccgagc actacaccga cgacgcgggc cacgaccgtg
     7441 tgcgctgggt gccgggacgg gtcctcaagg gtgtcgccta cgacacgccg atccagggct
     7501 acggcgtgaa cacctgcaac gtgctgacgt tgtggagcgc gcgcgccgtc aagtcgtttg
     7561 cgttggaggc gttcaacacc ggcgactatt acaaggcggt cgaggacgag gtcaactcgg
     7621 aaaccgtcac caaggtgctc tacccgaacg acgagcccga ggcgggcaag cggcttcgcc
     7681 tgctgcaaca gtactttttc gtgtcgtgtt cgctgcagca cgtcctgcac atcatggacg
     7741 atctggccga cgcgtccgtc aaggaccttc ctcagcgatt tgcgttgcag ctcaacgaca
     7801 ctcacccatc tatcggcgtc gccgaactca tgcggttgct ggtcgacgaa cgtcacatcg
     7861 actgggctga ggcctgggac atcaccgagg ccaccttcgg ctacaccaac cacacgctgc
     7921 tgccggaagc gctggagacc tggccgctgg cgatgttcgc cgaatcgctg ccccggcacc
     7981 tcgagatcat ctacgagatc aacaggcggt tcctcgacga ggtgcgcgcg aagtttcccg
     8041 gcgacgaggc ccggctgcgg cggatgtcgc tgatcggtga ggacggcggc aagagtgtcc
     8101 ggatggcaca cctcgctgcc gtcggcagcc acgccatcaa tggtgtcgcc gcgctgcatt
     8161 ccgagttgct caaagacagt gtcctcaaag acttttacga gctgtggccg gagcggttct
     8221 ccaacaagac caacggtgtc acaccgcggc gcttcctggc gctggccaat cc
//
LOCUS       DASRJA010000143        23666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3553184,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000143 DASRJA010000000
VERSION     DASRJA010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 23666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23666
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3553184"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VFC01_06590"
     CDS             complement(<1..670)
                     /locus_tag="VFC01_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79342.1"
                     /translation="MWRRLEVEDYDAVMALRQAVLSSLPDPDFYVREDDEHMFVRGHL
                     DVFGESVGSFEGDKLVAYLALTTDLVSSGEDVEFAACAPTVDDVVFAAAMVHPDYRGR
                     SLHRAGIRLHIAIAQALGAHRGMAQISARNHRSLRSYFSEGFRCTRAVEYPDGRRRLL
                     LERDLVDGTPAALIDDVALVDLNDFDSIRRELTDTRDGHRLLPVGPTALMVVGTARSR
                     TPVTA"
     gene            complement(654..3092)
                     /locus_tag="VFC01_06595"
     CDS             complement(654..3092)
                     /locus_tag="VFC01_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZN79343.1"
                     /translation="MTSATSHVVIISGNDSSLPVVPPPNTTITLIQTPDRATDFQRSA
                     ADQLIEVPEISAASLIELLRPIHETTPVTALACFLESAILAAAVAADALSIPSNPVDA
                     VRTAHNKFLTRQALKRHGIPQQPWRLCRSLDEVVAFRNSLAGAPIVVKPVTGAGSAGV
                     RLIRNDDDLRSAWDSIGGLKWWALLDNPENAVIAEAVLSGNEFSVEAMSVDGQHEILA
                     ITGKLTTGEPEFVELGHWQPAQLGDDQRDAVVNRTIAILDAIGHRTGPSHTEVMVDGL
                     DVGVIETHTRFGGDQIWELTQLTTGRHFATETIFALLGLPAPEPGDRHGAATMRKLDW
                     ADPDRIDDVEQREGVVRVSPPKQRRPEDVTAIADSSDLTGYVLTVGDDVHEAWTLAES
                     SCAAAKGESTTGASVLVMSPAKVVNHFGRERFAAAVPAGSVLITSGPVDVDASRLADL
                     VVFTDYTTDDTIPLVADEMIARHGLERIIVLAEADVLRAAEIRARHGIPGQQPDDALH
                     FRDKTLMKRVASAAGIAVAPHREVRSALELHDAAAELGYPCVVKPPHGRGSSGVSVLD
                     DAEALRGFLRTGPFSDQGRTSPWLVEAFQSGEQYRVDGVCRGGRVFLAAAAIYVNTHL
                     DFLGGGYMGSVMLPQESEEAQTVVGLARSVLEDALPAFDGGFHLEVFLTPEGPVFSEV
                     GARIGGGSIPEEVELSYGFNLVEEAILAQRGEPSRHTAPGQRSLAGQLNFSPVSGILA
                     EAPEKFEHPDVVLSEIASPGKSFASMTHTNAEFARAVFRADSVESGLDTISKLLQHMN
                     STTKWEVSDHVATA"
     gene            complement(3094..4605)
                     /locus_tag="VFC01_06600"
     CDS             complement(3094..4605)
                     /locus_tag="VFC01_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HZN79344.1"
                     /translation="MAMDRQREQMTTGLALVDAASVARIAIVGVGVGGTLTLSQLAEQ
                     ADSSWHGTTLDIVDRPESLGRGSAFGSDIAAAAVNTSQHRLDALSPSLHSFRSWLDES
                     GSPSTDLDTMPMSRSVYGDYLAATLAKAIDRLRDFGVRVNFYPMHATSVRPQGDGMAV
                     LGDAAELLPADIAVVAPGVWSPAPPPTRNVVQAYPLSGLQTRLGDQRSVAILGSGLTA
                     VDVACALDDGTTRVHMLSRSGTLPRVQVEAQKRYAPEHLTEDAVISEAVWSELTAKSV
                     LALLDRELARFGFTRSDIFSDAAHAAHWSDPLADPADIACHHTLSATNDAFNTAFTLL
                     PEPERVLLRESLGSKWFRYRVRVPLSRWRQLRAMQADGRLVVHGGVDAREGGLDDHLA
                     DLGTDRVIPALGQSSNLFEGPELVADLAASGLVDCDDAGQGLVDPATCRALAPGGGPR
                     DDLLLVGQVSAGSYFTVSALDVVVGQARRAAQTIADAITTRTSLRVPAARKAA"
     gene            4908..5335
                     /locus_tag="VFC01_06605"
                     /pseudo
     CDS             4908..5335
                     /locus_tag="VFC01_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
     gene            complement(5838..6893)
                     /locus_tag="VFC01_06610"
     CDS             complement(5838..6893)
                     /locus_tag="VFC01_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79345.1"
                     /translation="MKGIHHIRAAAAEAKARGTQRVDDVMRSAKAARPDTYDRSEEIV
                     RKSVAASMGLVRGGQDKVEDGYRRVEGTAPGAAAGSAVRRLGARARQIPLLTLPADAL
                     QERNGVRMLAENAAANPDCPMAHLWLGEALSALAKDQLVFNAARTVLNPLSALSREAM
                     RAVGRMGAGQDPAQQVLRRAYVLAVARLRKDKRDAVALHAIARIELANRRPDQAVLPA
                     RLAATASEGAARGAAFATLSRAYLSLGDDDRARRSAMAAIDAGCTIGWDTVAELLYRP
                     GEGPHANQDGSRQRQYVDIKSRVTDEDRRLYCGVHRDAAEITRSVLKSQSAKAKDGAA
                     QLRKGISQIGSRRRNKP"
     gene            complement(6912..7244)
                     /locus_tag="VFC01_06615"
     CDS             complement(6912..7244)
                     /locus_tag="VFC01_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79346.1"
                     /translation="MNEQELDFDGDDLLVPDADFDGFADAIVSAYVDAIADAEGATDL
                     VDADTGTNGTDGTDGTEVTYGEGGDAVFHNPDGSVSFSGSTLGGGDISYDSDLGVADY
                     SSGFDVDY"
     gene            7665..8639
                     /locus_tag="VFC01_06620"
     CDS             7665..8639
                     /locus_tag="VFC01_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HZN79347.1"
                     /translation="MLKLLGRLCAVVLGALLPVLAVVVVETPTAAAYSRKGLPVESLD
                     VPSPSMGRNIRVQFQGGGPHSVFLLDGLRAQDDANGWDINTAAFEWFYQSGISVVMPV
                     GGQSSFYSDWYQPATGSAGTVTYKWETFLTQELPSWLAANKAQAPTGNAVVGLSMSGS
                     AALTLAIWHPMQFIFASSLSGYLNPSMGLWPTLIGVSMKDAGGYNASNMWGPTSDPAW
                     RRNDPMVNINTLVANNTALWIYCGNGVTSELDDGANLGNQYSAQVLENITLSTNKEFQ
                     QKYIAAGGRNAVFNFPPDGTHSWGYWGSQLQAMKPDLVRIVGGQQPPS"
     gene            complement(8632..9390)
                     /locus_tag="VFC01_06625"
     CDS             complement(8632..9390)
                     /locus_tag="VFC01_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HZN79348.1"
                     /translation="MTDYPQMAAVRGRIEPAPRRVRGYLGSQLVFETTAARYVWEVPY
                     YPQYYIPLRDVRSEFLHDENHPQKVQLGPSRTFSLVGDGQTHQSAARVFDDGDGPVAG
                     LVRFDWEPLTWFEEDEPIYGHPRNPYVRVDALRSHRHVTVEVDGVVLADTHSPVLLFE
                     TGLPTRYYIDRTDVSFEHLEPSDTQTLCPYKGVTSGYWSVRVGDAVHPDLAWTYHYPL
                     PAVAPITNMVAFYNEKLDITVDGVKLAQPQTHFS"
     gene            9449..9634
                     /locus_tag="VFC01_06630"
     CDS             9449..9634
                     /locus_tag="VFC01_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79349.1"
                     /translation="MRVAIGIIGVVVALFGVVWALQGFGVLGGSPMSNTTTWSIIGPI
                     VAVIGIALAIGAFRRRK"
     gene            9763..10683
                     /locus_tag="VFC01_06635"
     CDS             9763..10683
                     /locus_tag="VFC01_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HZN79350.1"
                     /translation="MAVVLRPGDSNDLVRLLQARLNRDYPLYSNLVVDGIYGPRSTAV
                     VREFQRRAGLVVDGIAGPRTLGRLGLNFDQPPPLPTNLPFFYCASGTWATPFQGPPFD
                     VGWRVEQMGLLLRLILMNAGQFYLCGYSQGAEVVVRTLRLMLPGQPLAHRAGDLLKVI
                     TFGSPCRPPGPTLLGNNPPGAGISGIYTPEQFRSRTYDFVLNGDMYSTTTDDTLLHLG
                     YEALTPLELDLPFALKIFTLIQSNEFLALLNLANTPENFAKVVRTAIVVGDFLIRNPH
                     IHYGDWPDFNGVTAVNRAVQIVATGSTGGP"
     gene            10774..11760
                     /locus_tag="VFC01_06640"
     CDS             10774..11760
                     /locus_tag="VFC01_06640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HZN79351.1"
                     /translation="MTVLVFPGQGCQWAGMGCDLVDTSEVFAAQLRRCAEAIQAYVDW
                     SVEDVLRRRPGAPSLEHVEVVQPVLFSVYVALAELWMANGVVPQAVIGQSQAEIAAAC
                     VAGALSLEDAVHIMVSRSQLFADELVGRGGIASIGLAAREATDYLAPYQGRLEIAGII
                     GASSVTVAGELAALKSLVAQLSHRNIAAKAVPVSIPSHCAYIEPLHERLTALLARVRP
                     RPSRIPFYSTVTGDALRGDELTADYWYDNARRPVLFDPAMRVLLAHGHRVFVESSAHP
                     VLRRAMTDTAGDVGCPVVVTGTLRRGRSGPDEFRSALAAVPTQHAAPVQTAA"
     gene            11899..12855
                     /locus_tag="VFC01_06645"
     CDS             11899..12855
                     /locus_tag="VFC01_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79352.1"
                     /translation="MRVIFLQFDQRAQRQRSILRLALVTIMVLAVFSGTSRSEWPAQF
                     VLVAVYGALSIVAAWMWLRHPHRARKLRAIEPMVPVDIAAICVLPFMSTGSYLMLGLL
                     AFLPFFIATQAGRRAAVMSVAAIVGGGVGVLTDPVFRQDLSPLAIVTILTMLALLCLC
                     SYTVSRTQQRRLASIAELTASRSLLLADVMTAEERERRSIAEALHDGALQTVLAAKQD
                     LREAIRANGDRSGIERASELLVDVSHDLRQVTRELHPSVLDEAGLAAAVQSLADSFSE
                     RTGVPVECSIDYPQRHVFDATLYGAARELLNNVARHADAHRV"
     gene            12862..13083
                     /locus_tag="VFC01_06650"
     CDS             12862..13083
                     /locus_tag="VFC01_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN79353.1"
                     /translation="MSDRGDVVTLDVRDDGVGMDPSIVARRLSEGHIGVASHRARIET
                     LGGTVEFEPGERGTWVRVRLPIRVGQEAQ"
     gene            13080..13499
                     /locus_tag="VFC01_06655"
     CDS             13080..13499
                     /locus_tag="VFC01_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN79354.1"
                     /translation="MTKSDRVRVVVGDDHPVYRDGLVHALDNNGRTEVVVAVGDGRAA
                     LDAIREHSPDVALLDYKMPGLDGIAVAHAVVRDKLPTRVLLVSATTEGKSVPDMAAEL
                     YLAQTTIKTHIRRLYEKLGVSDRGAAVAQAMRNHMLE"
     gene            13598..14314
                     /locus_tag="VFC01_06660"
     CDS             13598..14314
                     /locus_tag="VFC01_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HZN79355.1"
                     /translation="MTTTIPTTPIYPSTKPVTRARNAKPVAHPTAMKYAVEMIGTLFL
                     VFTIGTAVRSGNPLAPLAIGAVLMAMVYAGGHISGAHYNPAVTVAVLIRGRIEIREAV
                     GYWIAQIVGGLLASALVSAVAPAQTSPMNVSGHALAAAFIAELLVTFALCYVVLNVAT
                     SKDHPDNSFYGLAIGFTVAAGAVAVGGISGGAFNPAVAISGVAMGMFSGSMIWMFVAS
                     QLVAAVAAGLAFRRLNPADK"
     gene            14396..14809
                     /locus_tag="VFC01_06665"
     CDS             14396..14809
                     /locus_tag="VFC01_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HZN79356.1"
                     /translation="MWNEWWAIALGIVGGLVLVWVVLVAVLWRTKPGASDLKEALRLL
                     PDLIRLIKRLAADPDTPRGVRIRLALLLGYLALPFDLIPDFVPVLGYADDAIIVALVL
                     RSTTRAAGPDALAKHWPGTPEGLTALKRLCRLNGK"
     gene            15318..16313
                     /locus_tag="VFC01_06670"
     CDS             15318..16313
                     /locus_tag="VFC01_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79357.1"
                     /translation="MDPEIALDGTVAVRRGPIGDIAASGVAAIVTNYGDDSVSFLNAE
                     TLAAEDTIGVPGEPFAAVISNDRAYVTSSSASHDAVSVIDTNTRTVIATYPLAFGVTA
                     LAISPDGKRVYAGRTGNDHVDVAVIDTTAERVGTIDIATGAGIGVDAIAVDPTGKRLY
                     VATTDARGSQLVVVDAETARVERKVWVGAPIRDLAFADGTAYVLTSDRVRGGAVSVID
                     MSSNRITDTIELGIGAPTQMTLSPDKSRAYVVDYDHVAVLCTLSHEVVTTVSVDARPS
                     CVTVDSDGGRLYVADYAGDVSVFSVASTLPQLYSRLVATEPIALAAPRELEPATA"
     gene            16430..17494
                     /locus_tag="VFC01_06675"
     CDS             16430..17494
                     /locus_tag="VFC01_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN79358.1"
                     /translation="MPGPKTNAPAIDFGAQGGDPSRGVDMDLSTCVNRYGPAPAAIAA
                     LHAIEPADIVLHPYQAAPQLIELYREATGVSHGAMIAGRGASEFIWAMCRELDHASVQ
                     VPMPAYTDYLKAFPGRGFDPRGSQIPSLEQIDSALNAAGVVIISNPHNPTGAHLDPGG
                     LIAVAAAHPAATLVVDESYIDFTPDPAGWSVLGCELPNIVVLRSTTKFYGIAATRTGI
                     AWCAESEPLAYLFGQQENWGLSGVDVRIACAAVRDADWSDRSRAQMHDDNAWLAEMLS
                     GVGGLDLHANKNVHFQFAFCERSLEVADVFLRHGIGVRVLGAAHGVRPDALRIVAPRH
                     DERERFVDALTEVVDERAAA"
     gene            17475..18428
                     /locus_tag="VFC01_06680"
     CDS             17475..18428
                     /locus_tag="VFC01_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN79359.1"
                     /translation="MNAPRHDRIGVVGVGLAAVGAVAYGIATVIGRALAASGVDSATA
                     LGIRFSVAAIILTVLIWLRGAPFRPGTGEWLPIVLLGAIGYTLESTLFYLSLRHGTAA
                     ACILLFYAYPAIVTVIEFARGREQITKPTAGALLLSVVGTASVVAVGRDVSISATGVI
                     LALAAATAYGLYLVVGRSVRRQTDSMTAACWVAVGAAAASLMRGVVGGTLQIPTGHVV
                     QIVGYGAVTAAAFALTFAALARIGATQTAVVMTLEAAATVLLAGLVLGEGISSVQALG
                     GVAILTAAAIIAHSHRRERAQNVRGMRRVDVQTRTLAGIGD"
     gene            complement(18425..19114)
                     /locus_tag="VFC01_06685"
     CDS             complement(18425..19114)
                     /locus_tag="VFC01_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN79360.1"
                     /translation="MISVLIVEDEPLIAEAHRTYLGRLSGFSVAAVAHTARDAMRAAS
                     EAMASETPIDLVLLDIGLPDANGIALASGLSGLRPAPDIIAITSERDLEMVRAAVGHG
                     ALAYLLKPFTFAAFRDRLERYKRYREALPAGTDAASQAEVDRALAELRVGADRSAAPK
                     GAAPGTNDEIARAVRDSADGITADEVAKQVGVSRVTAWRYLERLADEATVTRQTDYGK
                     AGRPKTRYTWR"
     gene            complement(19111..20658)
                     /locus_tag="VFC01_06690"
     CDS             complement(19111..20658)
                     /locus_tag="VFC01_06690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZN79361.1"
                     /translation="MNKFWPRSLAGQAIALQILVIAVVVLAGSALALFDAARDGDAAA
                     RQQVLGIATALADSPSTAAAIQSGNATATLQPVTEAVRKNTDIAFITIMAPDRTRFTH
                     TDPNQIGGKYIGTIEPALQGETFTEVYTGTLGPSIRAVAPVRDQTGRIVGLVSAGILQ
                     QSLADRWRSQVPTIVAVSLAALAIALVGVWAIRRRLLRQTHGLRPDELRVMYEHHDAI
                     LHSVSEGLIVLDRNGVALANDEARRLLALPPGPVDRSDLPEFLRTYNPGARDEVHVTA
                     ERVLVVNRSRVEDAPRDSEVVTIRDRTELQGALGELSSLKVLTDTLRSQAHEAANKLH
                     TIVTMVEMGRADEAVKFATNELELSQQLVDRLSSTVSEPALVALLLGKTAQADERGIE
                     LTVTEETHLPSNADDLALSGQEMVTVLGNLIDNAMDACDREDPWVEVTVSQDDGTLLI
                     RVADSGPGMDAATFEKAMQRGYSTKDDSEQHGLGLALVAQVVQRHNGTLTADVTYGSV
                     VTVTVKS"
     gene            20870..21370
                     /locus_tag="VFC01_06695"
     CDS             20870..21370
                     /locus_tag="VFC01_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79362.1"
                     /translation="MMRDRSSYLVCLCAVSLAIASLLLPTASAEPASWNGEYAITFIV
                     GPKEGSSIAAGQSEVQYTDTYGFSSSCDSGKCTATIISGPPPRNPTVPQPIQFTWDGS
                     SWTQATDFQWDCMMPDTTIQWSPARGDVRYTPQPDGTLAGTMHTEIMSGPCQGTLDIH
                     MTAERV"
     gene            21753..22121
                     /locus_tag="VFC01_06700"
     CDS             21753..22121
                     /locus_tag="VFC01_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN79363.1"
                     /translation="MMIRNLAMSGISVVGALGIAAPANADQYDFISVLDNNGVYYYNI
                     SEMIDLGKAACRTVRTGGTIGSVDSAINLLTRAGFNAVMDRAIIMPAAANTMCPDIWP
                     ALNAHNQAARDLLQGTAPAN"
     gene            22249..23631
                     /locus_tag="VFC01_06705"
     CDS             22249..23631
                     /locus_tag="VFC01_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HZN79364.1"
                     /translation="MTTHLDRPPVEEPPRRRDRTHWLYIAVIVAVLAGIAVGLVAPEV
                     GKSVGVLGTMFVSLIKMMIAPVIFCTIVLGIGSVRKAATVGKVGGLAFVYFLVMSTFA
                     LAIGLVVGNLIHPGSGMHLSETAAGKGAELAEKAHESGGLMDFVQGIIPDTLFSSLTA
                     GSVLQALFVALLVGFALQAMGSAGQPILRGIEHLQKLVFKVLVMILWLAPIGAFGAIA
                     NVVGQTGWTAVTQLLTLMLGFYVTCVLFVFGVLGVLLRVVSGISIFKLVRYLAREYLL
                     IFSTSSSESALPRLIAKMEHLGVDRSTVGVVVPTGYSFNLDGTAIYLTMASLFIADAL
                     GSPMSVGQQIGLLVFMIVASKGAAGVTGAGLATLAGGLQSHRPDLLDGVGLIVGIDRF
                     MSEARAVTNFSGNAVATVLVGSWTKTIDRDQVNAVLGGEDPFDELTMVDDDRTSHAEE
                     PAQERVPVPA"
BASE COUNT         3976 a         7690 c         7693 g         4307 t
ORIGIN      
        1 tcgcagtgac aggtgtgcgc gaacgagcgg tgccgaccac catcagagct gtgggaccga
       61 ccggaaggag gcgatgtccg tctcgggtat cggtcagctc acgacggatg ctgtcgaagt
      121 cgttcaagtc gacgagtgcg acatcgtcga tcagcgccgc cggggtgcca tcgacgaggt
      181 cgcgctcgag aaggaggcgc cgacgaccgt cgggatactc gaccgcccgt gtgcaccgaa
      241 agccttcgga gaaatacgac cgcagcgagc ggtggttccg cgccgagatc tgcgccatac
      301 cgcgatgcgc tcctagcgcc tgggcgatgg cgatgtggag gcggatgccg gcccgatgca
      361 gcgaacggcc gcgatagtcg gggtggacca tggccgcggc gaacacaacg tcgtccacag
      421 tcggtgcgca ggccgcgaac tcgacgtcct cgcccgaaga caccaggtcg gtggtcagag
      481 ccaggtatgc gaccagtttg tctccctcga aagatccgac ggattcgccg aacacgtcca
      541 ggtggccgcg gacgaacatg tgctcgtcgt cctcgcggac gtagaagtca gggtcaggaa
      601 ggctgctgag aacagcctgg cgtagcgcca tgactgcgtc gtagtcttcc acttcaagcc
      661 gtcgccacat gatcagagac ctcccatttc gttgttgagt tcatgtgctg cagaagtttt
      721 gagatcgtgt ccaggccgga ctccacggag tcggcgcgga acaccgcccg agcgaactcg
      781 gcgttggtgt gagtcatcga agcgaagctc ttccccggtg aagcgatctc cgacaggacg
      841 acatctggat gctcgaactt ctccggagcc tcggcgagaa tccccgacac cggagagaag
      901 ttcagttgtc ccgccaggct gcgctgtccc ggcgcggtat ggcgcgaagg ctccccgcgc
      961 tgggcgagaa tcgcttcctc gacgaggttg aacccgtagg agagttcgac ctcctcaggg
     1021 atggaaccgc caccgatccg agccccgact tcgctgaaga ccggaccctc cggtgtgagg
     1081 aacacctcga gatggaatcc accgtcgaac gccggcaggg catcctcgag aacagaccgg
     1141 gccagtccga cgacggtctg tgcctcttcg ctctcctgag gaagcatgac ggagcccatg
     1201 tatcctccgc cgaggaaatc gaggtgggtg ttgacgtaaa tcgctgcggc ggcgaggaat
     1261 acgcgaccgc cacgacacac tccgtcaacc cgatactgtt cgccggattg gaaagcctcg
     1321 accagccacg gagacgttcg tccttgatcg ctgaacggac cagtgcgcag gaagccgcgc
     1381 agtgcctccg catcgtcgag cacggacacg ccagaggatc cgcgaccatg cgggggtttg
     1441 accacgcacg ggtaaccgag ttcggcggcg gcatcgtgaa gctcgagcgc tgaccgaact
     1501 tcacggtgcg gggccactgc gataccggcg gcgctcgcca ctcgcttcat cagcgtcttg
     1561 tcgcggaagt gcagcgcgtc gtcgggctgc tgcccgggga ttccgtgccg agcacggatt
     1621 tcggctgctc gcaagacatc tgcctcggcg agcacgatga tgcgctcgag gccgtggcga
     1681 gcgatcatct catccgccac gagggggatc gtgtcgtcgg tcgtgtagtc ggtgaacacc
     1741 accaagtcgg ccaaccgaga ggcgtcgacg tccaccggac cggacgtgat gaggacggat
     1801 ccggcgggta cggccgcggc gaaccgctcc cgaccgaagt ggttgaccac cttggctggg
     1861 ctcatgacca aaacagatgc gccagtggtg ctttcgccct tcgccgcggc gcaggacgat
     1921 tccgccaagg tccacgcctc gtgcacgtcg tcgcccaccg tcagtacgta gccggtgagg
     1981 tccgacgaat cagcgatcgc ggtgacgtcc tcgggccgac gctgctttgg tggggacacc
     2041 cgcaccacac cttcccgctg ttcgacgtca tcgatccggt cgggatcggc ccagtcgagc
     2101 tttcgcatcg tggcggcgcc gtgccgatcg cccggctcgg gcgcgggcag accgagaagg
     2161 gcgaagatgg tctcggtggc gaaatggcgt cccgtcgtga gctgggtgag ctcccaaatc
     2221 tggtcgccgc caaagcgtgt gtgggtttcg atgactccaa cgtcgagccc gtcgaccatc
     2281 acctcggtgt gactcggccc tgtccgatgc ccgatggcat cgaggatcgc tatagtccgg
     2341 ttgacgacag cgtcccgttg gtcgtcgccg agctgtgccg gctgccagtg gccgagttcg
     2401 acgaactcgg gctccccggt ggtgagcttc ccggtgatgg caaggatctc gtgctgtccg
     2461 tcgacgctca tcgcctcgac actgaactcg ttgccggaca acaccgcctc ggcgatcacc
     2521 gcgttttccg gattgtccag gagcgcccac catttgagcc ccccgattga gtcccatgcg
     2581 gaacggagat cgtcgtcgtt acggatcaac cgaacaccgg cactgccggc gcccgtcacg
     2641 ggcttgacca cgatgggcgc acccgccaac gagttccgga atgcgacgac ttcgtcgaga
     2701 ctgcggcaca gacgccatgg ttgctgggga atgccgtgtc gtttcagggc ctgccgggtg
     2761 aggaacttgt tatgggcggt gcggacggcg tcgaccgggt tcgacgggat actcagcgcg
     2821 tcggcagcga ccgccgccgc caagatggca gattccaaga agcacgccag cgcggtgacg
     2881 ggtgtcgtct cgtggattgg ccgcagcagt tcgatgagcg acgccgcaga gatctccggc
     2941 acctcgatca attgatccgc tgcggagcgc tggaaatctg ttgccctatc gggcgtctgg
     3001 atcaacgtga tcgtggtatt cggcggcggc acgaccggaa ggctggagtc gttgccgctg
     3061 atgatgacca cgtgtgaagt cgcggaagtc atgtcaggca gcctttctcg ccgccggcac
     3121 acggaggctt gttcgggtgg tgatggcatc ggcgatcgtc tgcgcagcgc ggcgggcctg
     3181 tccgacaacg acatcgagcg cagacaccgt gaagtagctg cccgcggaaa cctggccaac
     3241 gagcaagagg tcgtcgcgcg gccctcctcc gggggcgagg gcccggcacg tcgcgggatc
     3301 gaccaggccc tggccggcat cgtcacagtc gacgaggccc gacgcggcta ggtcggcaac
     3361 cagttcgggg ccttcaaaga ggttcgagga ctggcccaaa gcggggatca cgcggtcggt
     3421 tcccaggtcg gcgagatggt cgtcaagccc tccctcgcgg gcgtcgacgc ctccgtggac
     3481 caccaggcgt ccatcggcct gcatggcacg aagctgccgc caccggctca gtggaaccct
     3541 gacccggtat cggaaccact tggaccccag cgactcacga agaaggaccc gttccggctc
     3601 gggcagcagt gtgaatgcgg tgttgaacgc gtcgttggtg gcgctgagtg tgtggtggca
     3661 ggcgatgtcg gccggatcgg cgagcggatc tgaccagtga gcggcgtggg ctgcatcgga
     3721 gaagatgtcg gatctggtga atccgaaacg agcgagttcg cggtccagca gggcaagcac
     3781 cgacttcgcg gtcagctcac tccagaccgc ctcagagatg accgcatcct cggtcagatg
     3841 ttctggtgca tagcgctttt gggcctccac ctgaacccgg ggcagggtgc cgctccggga
     3901 cagcatgtgg acacgcgtcg tgccgtcgtc aagcgcgcag gcgacatcca cggcggtgag
     3961 gccgctgccg agaatcgcga ctgaacgctg atcgccgaga cgagtctgca agccactgag
     4021 ggggtaagcc tggacaacgt tgcgagtcgg aggcggagcg ggagaccaca ctccaggagc
     4081 taccaccgcg atatcggccg gcagcagttc ggcggcatcg ccgaggacgg ccattccatc
     4141 gccttgtggc cgtaccgacg tggcgtgcat cgggtagaag ttgacgcgga ccccgaagtc
     4201 gcgcagacgg tcgatcgcct tcgccaaggt cgccgcgagg taatcgccgt acaccgatcg
     4261 ggacatcggc atcgtgtcga ggtcagtcga cgggctgccg gactcgtcaa gccagctgcg
     4321 aaacgaatga agcgacgggg acagtgcgtc gagccggtgc tgggaggtgt tgaccgcagc
     4381 ggccgctatg tcgctaccga aagcgctgcc acgcccgagg ctctctggcc ggtcgacgat
     4441 gtccagcgtg gttccatgcc aggagctgtc cgcctgctcc gcgagttgac tgagggtgag
     4501 cgtgcctccg acgccaactc caacgatcgc gatgcgcgcg acgcttgctg catcgactag
     4561 cgcaagcccc gtcgtcatct gttcccgttg gcggtccata gccatggggg aaatactcgt
     4621 gatcaggcaa tggcggcgta atcgccacga agccagactc aactgtcgaa gaggaagagt
     4681 ttcctatccg cggggtaagc caccaagaag atgaccgggt gaggagcgga atgcctatga
     4741 gatcgaagac atgccgaagt gccgccgttc tggccggccg cgccgtcgaa tccgttgccg
     4801 atcaacagtc cggcttgact acttcgtttc ctttcaccag ctgtcgagaa tcgcttgccg
     4861 aggtgcgtag tttcgggtca tccaacactt gcgaagggaa atgcccagtg gctcagtcag
     4921 gatcgtggac gggcgacccg gtctggctcg cggacgtgct gcgcgccgag ggtgtccggc
     4981 tcgtcgagta tcccggttgg cgcgaccgcg gccacggtga cttcaaggac attcctggcg
     5041 tcatggtgca ccacaccgga tcggacaacg ccacggcagc ttccatcgcc atcggcaggc
     5101 ccgagtacgc cgatgttctg ttgcatcgcg gttccgaagg gcccgaggtc gccgaactcc
     5161 aacgtcggct cacgaagagg agggcaccga cctggttgtc gacgggatct tcggttcgaa
     5221 gaccgaagcc gccgtccggg catttcagcg aaggaccggt ggcctcaagg tcgacgggat
     5281 cgttggaccg gcaaccgctg cggcgctgag gttgaaactc ttgcaagccg cgtagtcacc
     5341 gatcggcgcc gtcagataca cagcacggca aacaaatggg tgcagcggcg gcatgtcgga
     5401 gtaccgtcat gcccaccaag gggttcgggt cgatattgct agaggtacgt cgatgaaatg
     5461 caaatatttg ccgctgctgt gctgtctgcc ggcgtgctag cagtcggatt cagcgcggct
     5521 gcagcaaacg ccaatccgct cccgcctccg cctccacctg tcttcccaac tctgcctgta
     5581 ccggcgcctc caccgttcat gcttccggct gcggacccgg ttccagcccc gtttgtgccg
     5641 cccccgcttg cggctccgcc cccgcttgtg ctggctccgg ttgcgccgcc ggcggccgat
     5701 gtcatacagg ttccgatcgg catacccata aacatctgcg gcaactcaat cggcatctgc
     5761 gcggtaccga tttgtattcc gggagcctcg ggatgtgctg acctgcccga cgactgactc
     5821 ggagggtagc cgattcctta gggcttgttg cggcgacgcg agccaatctg gctgatgcct
     5881 ttcctgagtt gggccgcacc atctttcgcc ttggcggact ggctcttcag caccgagcgg
     5941 gtgatctccg cggcgtcccg gtggacaccg cagtagagcc gccggtcttc gtccgtcacc
     6001 cgtgacttga tgtcgacgta ctggcgttga cggctgccgt cctggttggc gtgaggaccc
     6061 tcgccggggc ggtagagcag ctcggccacg gtgtcccagc cgatggtgca ccccgcgtcg
     6121 attgccgcca tggcactgcg ccgggcgcgg tcgtcgtcgc cgaggcttag ataggcacgc
     6181 gacaaggtcg cgaatgcggc tccgcgcgcg gcgccctcgg atgctgtggc cgccagtcgg
     6241 gccggcagga ccgcttgatc gggccgtcgg ttagccaatt cgatgcgagc gatcgcatgc
     6301 agcgctaccg catctcgctt atctttgcgc agccgggcca ccgcgaggac gtacgcgcgg
     6361 cgaagcacct gttgcgctgg atcttgcccg gcacccattc gtcccaccgc ccgcatcgct
     6421 tcccgtgaca gcgccgatag ggggttgagg accgtgcggg cggcgttgaa caccagctga
     6481 tccttcgcga gcgcagacag cgcctcgccg agccaaaggt gcgccatcgg acaatcgggg
     6541 ttggccgcgg cgttttcggc tagcatccga acgccgttcc tttcctgcaa ggcgtccgcg
     6601 ggcaacgtca gcagcggaat ctgccgggcg cgggccccga gccggcgcac cgcgctaccg
     6661 gctgccgccc cgggggccgt gccttcgacc cggcgatagc cgtcctcgac tttgtcctga
     6721 ccgccccgga ccaaacccat agacgctgcg actgacttcc ggacgatctc ctcggatcga
     6781 tcgtaggtgt ccggtcgcgc cgctttcgcg cttcgcatga cgtcgtccac ccgttgcgta
     6841 ccacgcgcct tggcctcagc cgcagcagcg cggatgtgat gaatgccctt caccgtcggt
     6901 ccgcccgccg ctcagtagtc cacgtcgaag ccagacgaat agtccgcgac gccgagatcg
     6961 gagtcgtagg aaatgtcgcc gccaccaagt gtgctgcccg agaacgacac cgaaccgtcg
     7021 ggattgtgga agacggcgtc accgccctcg ccgtaggtga cctcagtacc gtctgtaccg
     7081 tctgtgccgt ttgtgccggt gtccgcgtcg acgaggtcgg tggccccttc cgcgtccgcg
     7141 atggcgtcga cataggcgct gacgatcgcg tctgcgaagc cgtcgaagtc cgcgtcgggt
     7201 accagcaagt cgtcgccatc gaagtcgagt tcttgttcgt tcattgccat gccctttctg
     7261 caagctgcga gtacatcgac cgtcgacgta ttcgttcgct cagtgttcag gcaaggactc
     7321 aagaatttct aaaggccgtc cttgggtgcc gatcgcagca gatcagcgcc ggctcaacag
     7381 acgtggcgcg ctgcgcaggc cgtcgccgcc gatcaattgc cgtaccaagg tccgtcctga
     7441 cctccgtgtc cgatccacgc tttggtggtc ccgctgagac ttggatgaag cggttgcgct
     7501 caaccgccgg tcggcgcgcc tcctcgggtt gtgtggtcgg tgacgcattt tcctttcgtt
     7561 cgaaaggttt cagcgaggcc gcaactttga taacaagctg gtcaagcggc gcgcgccgcg
     7621 cagaaacgcg tcactctcgc tgttagcttg ccggggtggt tgcgatgctg aagttgcttg
     7681 gcagactctg cgcggtggtg ctgggagcac tcttgccggt gctcgccgtc gtcgtggttg
     7741 aaacgccgac ggccgcagcg tattcacgta aagggctccc ggtggagtcc ctggacgtgc
     7801 cgtcgccatc gatgggccgc aacatccgcg tccagttcca gggcggcgga ccgcattccg
     7861 tctttctgct cgacgggctt cgggctcagg acgacgccaa cggctgggac atcaacaccg
     7921 cggcgttcga gtggttctac cagtccggaa tctccgtcgt catgcccgtc ggcggacaat
     7981 ccagcttcta ctcggactgg tatcaacccg cgacaggaag cgcgggaacg gtcacctaca
     8041 agtgggagac ctttttgacg caggaactgc cgagctggct ggccgccaac aaggctcagg
     8101 cgccgacggg caatgccgtt gtcggactgt ccatgtcggg cagcgcggcg ctgaccctgg
     8161 ccatctggca tccgatgcag ttcatcttcg ccagctcgct gtccgggtac ctgaacccat
     8221 caatgggctt gtggcccacg ctgatcggcg tttcgatgaa ggacgccggt ggctacaacg
     8281 cttcgaacat gtgggggccg acgtccgacc ccgcgtggcg ccgcaacgat ccgatggtca
     8341 acatcaacac cctggtggcc aacaacacgg cgctgtggat ctactgcggc aatggcgtca
     8401 cgtcggaact tgacgacggg gcgaatctcg gcaaccaata cagcgcccag gtcctggaga
     8461 acatcacgct gtcgaccaac aaggagttcc agcagaagta catcgcagcc ggcggtcgca
     8521 acgcggtgtt caacttccca cccgacggca ctcacagctg gggctactgg ggctcgcagc
     8581 tgcaggccat gaagcccgac ctggtgcgca tcgtcggcgg gcagcagcca cctagctgaa
     8641 gtgcgtctgc ggctgcgcaa gcttcacgcc gtcgacggtg atgtcgagct tctcgttata
     8701 gaacgcgacc atgttcgtga tcggcgcgac cgccggcagc gggtagtggt atgtccacgc
     8761 gaggtcggga tggacagcgt cgcccacgcg caccgaccag taacccgacg ttacgccctt
     8821 gtacggacac agcgtctggg tgtccgacgg ctccaggtgc tcgaaggaaa catcggtccg
     8881 gtcgatgtaa taccgtgtcg gcaaaccggt ttcgaacagc aatacgggac tgtgagtgtc
     8941 ggcaagcacc acgccgtcca cctcgacggt gacgtggcgg tgtgagcgca gggcatcgac
     9001 ccggacgtac gggttgcgcg gatgaccgta gatcggctcg tcctcctcga accacgtcag
     9061 tggttcccag tcgaaacgca ccagcccggc gacggggcca tcaccgtcgt cgaacacgcg
     9121 tgccgccgac tggtgcgtct gaccgtcgcc aaccaacgag aacgtccgcg acgggccgag
     9181 ctgcaccttc tgcgggtggt tctcgtcgtg taggaactcg gatcggacgt cgcgcagcgg
     9241 gatgtagtac tgcgggtaat acgggacctc ccacacgtac cgcgccgccg tcgtctcgaa
     9301 gaccaactgg cttccgaggt agccgcggac ccgccgcggc gccggttcaa tacgtccgcg
     9361 aaccgccgcc atctggggat agtcggtcat gggccgctgg gatgtggtgc tcaccgttcc
     9421 ataatggtct cgcgcctaca gtgaaggaat gcgggtagcg attggcatta tcggcgtggt
     9481 cgttgcgttg ttcggcgtgg tgtgggccct gcaggggttc ggtgtcctcg gcggcagccc
     9541 gatgagcaac accaccacct ggtccatcat cggtccgatc gttgcggtga tcggcatcgc
     9601 cctcgcgatc ggcgccttcc gccgccgcaa atagttcgcg cagatcgacg agcacgacta
     9661 aaccgcgacg gcaacgccct cgcgcgatgc cgcaccaggt tgctccgtcg tagcgggacc
     9721 aaatccgtta caggcgaatg gaatccggcg tagcgtcgat gtatggcagt tgtattgcgc
     9781 cccggtgaca gtaacgatct tgtccggcta ttgcaggccc ggctgaaccg cgactatccg
     9841 ctgtactcaa acctcgtcgt cgacggcatc tatgggccgc gcagcaccgc cgtggtccgc
     9901 gaattccagc gccgcgccgg tctggtggtc gacggcatcg ccggaccccg caccctcggg
     9961 cggcttgggc tgaacttcga ccaacccccg ccgctgccca ccaacctgcc gttcttctac
    10021 tgcgcctccg gtacctgggc cacaccgttc caaggaccac cattcgatgt cggctggcgt
    10081 gtcgaacaga tgggactact gctgcggctg atcctgatga acgccgggca gttctacctc
    10141 tgcggctata gccagggcgc tgaggtggtg gtccgcacgc tgcggctgat gttgcccggc
    10201 cagccgttgg cgcaccgcgc cggggacctg ctcaaggtga tcacgttcgg cagcccctgc
    10261 cggccgcccg gcccgacgct gctcggcaac aatccgccgg gagccggcat ctccggtatc
    10321 tataccccag aacagttccg ttcgcgcaca tacgatttcg tcctgaacgg cgacatgtac
    10381 agcacgacca ccgacgatac gctgctgcac ctcggctatg aggcgctcac cccgctcgaa
    10441 ctcgatctgc cgttcgcgct gaagatcttc acgctgatcc agagcaacga gttcctcgcc
    10501 ctgctcaacc tcgccaacac gccagagaac ttcgcgaagg tcgtcaggac cgcgatcgtc
    10561 gtcggcgact tcctgattcg caacccccac atccactacg gtgactggcc cgatttcaac
    10621 ggggtcaccg ccgtgaaccg cgcggtgcag atcgtcgcga ccggttcgac gggcggccct
    10681 tagcgtcatc cgttcggatg aacggcggtt catcccgtaa gtcgtgcaat tgcgcgctgc
    10741 cggacggacg tgacccccgg cagtgtcgtc ggcatgacag ttctcgtgtt tcccggtcag
    10801 ggctgccaat gggcaggcat gggctgcgac ctcgttgaca ccagcgaggt attcgctgct
    10861 caactgcgga gatgcgccga ggcgattcag gcatacgtcg actggtcggt tgaggatgtg
    10921 ttgaggcggc ggcccggcgc cccttcgctc gagcacgtcg aggttgtcca gccggtcctc
    10981 ttctcggttt acgtcgcgct ggcggagttg tggatggcca acggcgttgt tccgcaagct
    11041 gttatcgggc agtcccaggc tgaaatcgca gcggcctgcg tggccggcgc gctcagcctc
    11101 gaggacgccg tgcacatcat ggtttcgcgc tctcagctgt tcgccgacga actggtcgga
    11161 cgtggcggga tcgcgtcgat cgggctggcg gcgcgtgagg ccactgacta cctggccccg
    11221 taccagggca ggcttgagat cgcgggaatc atcggcgcca gctccgtgac cgttgccggt
    11281 gaactcgccg ccttgaaaag ccttgtggca cagctcagcc accgcaatat cgcggccaag
    11341 gcggtgccgg tgtcgatccc gtcgcactgc gcctacatcg agccgctgca cgagcgcctg
    11401 actgcgctgc ttgcgcgagt gcggccgcgg ccgagccgca tccccttcta ttcgacggtg
    11461 accggcgatg cgttgcgagg tgacgagctc accgcggact actggtatga caatgctcgt
    11521 cgtccggtgc tattcgaccc ggcgatgcgg gtgctgctcg cgcacggcca tcgcgtcttc
    11581 gtcgaatcga gcgcccaccc ggtgctcagg cgggcgatga ccgacaccgc cggcgacgtc
    11641 ggttgcccag tcgtcgtcac cggcacgctc cgccgtggtc gcagcggccc cgacgagttc
    11701 cgcagtgcgc tcgcagccgt gccaacccag cacgccgcgc cggtgcaaac ggcggcctga
    11761 cctgcgatat ggccgctgtt tgggcgctac actcccggtg tgcgccacga cggcagcgca
    11821 gtgccacggt caccaagccg attctggtcg agcttgctgg tgccgccgat cgcagtgggc
    11881 ccaactggaa gcgaagcggt gcgcgtcatt tttctgcaat tcgaccagcg ggcgcagcgg
    11941 cagcgcagca ttctgcggct cgcattggtc accatcatgg tgctggctgt gttcagcggg
    12001 acatcgcgtt ccgaatggcc ggcccagttc gtcctggtgg ccgtttacgg cgcgctctcg
    12061 atcgtcgcgg cctggatgtg gctgcgacac ccgcaccgcg cccgcaagtt acgggccatc
    12121 gaaccgatgg tgcctgtcga catcgccgcg atctgcgtgc tgcccttcat gtccaccggc
    12181 agttacctca tgttgggcct gctggcgttc ctcccgttct tcatcgcgac gcaggcgggg
    12241 cgccgcgcgg cggtgatgtc cgtcgcggcg atcgtcggcg gtggtgtcgg ggtactgacg
    12301 gatccagtgt tccggcaaga cttgtcgccg ttggcgatcg tgacgatcct gacgatgctg
    12361 gccctgctgt gcctgtgctc gtatacggtg tcacggacgc aacagcgcag gctggcgagc
    12421 atcgctgaac tgacggcaag ccggtcgctt ctgctcgccg atgtgatgac ggcggaggaa
    12481 cgagaacgcc gcagcatcgc ggaggcgttg cacgacggag cgttgcagac cgtgctcgcc
    12541 gcgaaacagg atctgcgcga agcgattcgg gccaatggcg acaggtcggg catcgaacgg
    12601 gccagcgagc tgctggtcga cgtgtcgcat gatttgaggc aggtcactcg cgaactgcat
    12661 ccgtcggtgc tcgacgaggc gggtctcgca gcggccgtcc agtcgttggc ggacagcttc
    12721 tccgaacgca ccggcgtgcc agtcgagtgt tcgatcgact atccgcagcg ccatgttttc
    12781 gacgcgacgc tgtacggagc tgcgcgagag ttgctcaaca acgtcgcccg tcacgcagac
    12841 gcgcaccggg tctagctgga gttgtccgac cgaggcgatg tcgtgacgct ggacgtgcgg
    12901 gacgacgggg tgggcatgga cccctcgatc gtcgcaaggc gactcagcga gggacatatc
    12961 ggcgtggcat cgcaccgtgc gcgcatcgaa acgctcggag gaacagtgga gttcgaaccg
    13021 ggcgaacgag gaacgtgggt gcgggtgcgg ctaccgatac gggttgggca ggaggctcag
    13081 tgacgaagtc ggatcgggtg cgggttgttg tcggcgacga tcacccggtc taccgggacg
    13141 gcctggtgca tgcgctcgac aacaacggcc gcacggaagt ggtggtcgcc gtgggcgacg
    13201 gcagggcggc gctcgacgcg attcgcgagc attcgcctga cgttgcgctg ctcgactaca
    13261 agatgccggg tctcgacgga atcgcggtgg cccacgcggt ggtccgcgac aagctgccga
    13321 cacgcgtgct gctggtgtcc gcgaccaccg aaggcaaatc ggtgcccgat atggccgcgg
    13381 agctctacct ggcgcagacc accatcaaga cacacatccg ccggctctac gaaaagctgg
    13441 gcgtatcgga tcgcggcgcc gccgttgcgc aggcgatgcg caaccacatg ctggagtaag
    13501 ccccagcgtt catccatttg gatgacaccc ggttcatccc cgtgctcgtg cgaatggacg
    13561 ctgccgctcg gctgacatgc gccgcaggct aatacgcatg acaacaacca tccccaccac
    13621 tcccatctac ccttcaacca aacctgtcac acgcgcacgg aacgccaagc ccgtcgctca
    13681 tccgaccgca atgaagtacg ccgtcgagat gatcggcacc ctgttcctgg tcttcacgat
    13741 cggcaccgcc gttcggagcg gcaatccgct tgctcccctg gccatcgggg cggtcctgat
    13801 ggcgatggtc tacgccggcg ggcacatctc cggcgctcac tacaacccgg cggtgacggt
    13861 ggcggtactg atccgcggcc ggatcgaaat ccgcgaggcc gtcggatact ggatcgcaca
    13921 gattgtcggc ggcctgctgg cgtcggcgct cgtctccgcc gtcgccccgg cgcagacgag
    13981 tccgatgaac gtgtccgggc atgccttggc cgcggctttc atcgcagaac tgctcgtcac
    14041 gttcgcgctc tgctacgtgg tgctgaatgt cgccacgagc aaggatcacc ccgacaactc
    14101 gttctatgga ctggcgatcg gcttcaccgt ggcggccggc gccgtggccg tcggcggaat
    14161 ctcgggtggc gccttcaacc cggccgtggc catcagcggt gtcgcgatgg gaatgttctc
    14221 cgggtccatg atctggatgt tcgtcgcctc ccagctagtc gcggcggtcg ccgccgggct
    14281 cgcgttccgg agattgaacc ccgccgacaa gtaaccacca cccacctggc gctccccctt
    14341 cgtcaacggc gacgacgggg gagtgccctg ttttcggagc ctggatcatt gagtcgtgtg
    14401 gaacgagtgg tgggcgattg cgctcggcat cgtcggcggg ctcgtcctgg tgtgggtcgt
    14461 tctcgtggca gtgctatggc ggaccaagcc aggcgcgtct gacctgaagg aagccctgcg
    14521 gctgctgccc gacctgatcc ggctgatcaa gcgcctggcc gccgatcccg acacgccgcg
    14581 cggcgtgcgg atccggctcg cgctgctgct cgggtacctc gcgctgccgt tcgacctgat
    14641 tccggacttc gtcccggtgc ttggctacgc cgatgacgcc atcatcgtcg cgctggtatt
    14701 gcggtcaacg acacgagccg ccgggcccga cgccttggcc aagcactggc cgggcacgcc
    14761 cgaggggctg acggcgctga aacggctgtg ccgcttgaac ggcaaatgaa gctctgtgat
    14821 ccagctaaca ccgcaacgcc cgtcgtgggt ttcccgtggc gtccacgctg gggccgtcgc
    14881 ccgaacgtgc ggggtacgtg acgggcgtcg agaagtttgc tgacgtgtcg gatgtgatct
    14941 accgctcact ttcgctccaa aacataagaa tccgttttcc gcagaacagc ggcagctgat
    15001 catgaatcga ttgccaggag gtcaaacgtg cacgttgacc aaggttatcg cgttgccata
    15061 ataaaataac gagtgcataa cgaagatttt cctaagcgct gtctgagagg attggcgcga
    15121 tttccgggga ttcgcaaagt cacccgcgcg taacacgaat tttcgtcgct acactcgacc
    15181 gagatcgcgc gaaaaggcgc agatagacct gcaatctagg gccggtgcac gccaaaccgg
    15241 tggaggtacc gataaagatg gctaactttt tgatgcctga gggcactgct gattctcatg
    15301 tgaatgacga ggcgcatgtg gacccggaaa tcgctctcga tgggaccgtc gcggtccgtc
    15361 gcggtccgat cggcgacatc gccgccagcg gcgtggccgc catcgtcacc aactacggcg
    15421 atgacagcgt ttcgttcctc aacgccgaga ctctcgcggc cgaggacacc atcggcgtcc
    15481 ccggcgagcc gttcgcggcc gtcatctcca acgatcgcgc ctatgtcacc tcgtcgtcgg
    15541 cgagccacga cgccgtgtcc gtgatcgaca cgaacaccag gaccgtcatc gcgacgtacc
    15601 cgctggcctt cggcgtcacc gccctggcca tcagcccgga cggcaagcgc gtctacgcag
    15661 ggcggactgg caacgaccat gtcgacgtcg ccgtcattga caccacggcc gagcgcgtcg
    15721 gcaccatcga catcgcgacc ggcgcaggca tcggggtcga cgccatcgcc gtcgacccaa
    15781 ccggcaagcg gctgtatgtc gccaccaccg atgcccgcgg cagtcagctc gtcgtcgtcg
    15841 acgccgagac cgctcgcgtt gagcgcaagg tgtgggtcgg cgcgccgatc cgggacctcg
    15901 ccttcgccga cggcaccgct tacgtcctca cgtccgatcg cgttcgcggc ggcgcggtca
    15961 gtgtcatcga catgtcgagc aaccggatca ccgacacgat cgaactcggg atcggcgcgc
    16021 ccactcagat gacgctgagc cccgacaagt cccgcgcgta cgtcgtcgac tacgaccacg
    16081 tcgccgtgct gtgcacgctg agccacgagg tcgtcaccac cgtttcggtc gatgcccggc
    16141 cgtcttgcgt cacagtggat tccgacggcg gtcggctgta cgtcgccgat tacgccggcg
    16201 atgtcagtgt gttctcggtc gcgtcaacgt tgccgcagct gtactcgcgg ctcgtggcca
    16261 ccgagccgat cgcgctggcc gcaccgcgcg agctggagcc agccacggca tagttagcct
    16321 gcgtggttac gcgccgacaa acaggtagtg aactacgtat gtccggcacg gccggtcttc
    16381 ctacggtcac aagcgcgatc gaaaaacgcg cgcagaccag gaggacatca tgcccgggcc
    16441 caagaccaac gcaccggcga ttgacttcgg cgcacagggc ggcgacccga gccgcggggt
    16501 cgacatggac ctcagcacct gcgtaaaccg gtatggaccg gcgccggccg cgatcgcggc
    16561 actacacgcc atcgagccgg ccgacatcgt gcttcatccg tatcaggcgg cgccccaact
    16621 catcgagctg taccgagaag cgaccggcgt ctcccacggg gcgatgatcg ccggccgcgg
    16681 tgccagcgag ttcatctggg cgatgtgtcg cgagctcgac cacgcctcgg tgcaggtgcc
    16741 gatgcctgcg tacaccgact acttgaaggc attcccgggc cgcggcttcg atcctcgtgg
    16801 cagccaaatc ccatcgctcg agcagatcga ttcggcgctg aatgccgctg gagtggtgat
    16861 catttcgaac ccgcacaacc cgaccggtgc ccatctcgat cccggcggac tcatcgccgt
    16921 cgccgccgcg catcctgctg cgacactggt cgtcgacgag tcgtacatcg acttcacacc
    16981 cgacccggca gggtggtcgg tgctgggctg cgagttaccc aacatcgtcg tgctgcgctc
    17041 gaccaccaag ttctacggca tcgccgcgac ccgcaccggc atcgcatggt gcgccgaatc
    17101 cgagccgttg gcataccttt tcggccagca agagaactgg gggctttccg gggtcgacgt
    17161 gcggatcgcg tgcgctgcag tgcgcgacgc cgattggtcc gaccgcagcc gtgcccagat
    17221 gcacgacgat aatgcctggc tggccgagat gctgtcgggc gtaggcggtc tcgacctgca
    17281 tgcaaacaaa aatgtgcact ttcaattcgc gttctgcgaa cgctcgctcg aggtcgccga
    17341 tgtgttcctc aggcacggaa tcggtgttcg tgtattgggc gcggcgcatg gcgtgcgacc
    17401 cgacgcactg cgcatcgtcg caccccgtca tgacgagcgg gagcgtttcg tcgacgccct
    17461 aacggaagtg gtcgatgaac gcgccgcggc atgaccgcat tggtgttgtg ggcgttggcc
    17521 tggccgctgt cggcgcagtt gcctatggca tcgcgacagt catcggccgc gcgcttgctg
    17581 cctccggcgt cgactccgcc actgcgcttg gcattcggtt ctccgttgcg gcgatcattc
    17641 tgacggtgtt gatctggctg cgcggtgctc cgtttcggcc aggcaccggc gaatggctgc
    17701 cgattgtcct gctcggcgcg attgggtaca cgctggagtc cacgctgttc tacctgtcgc
    17761 tgcggcacgg caccgccgcc gcgtgcattc tgctgttcta tgcataccct gccatcgtca
    17821 ccgtgatcga attcgcccgg ggcagagagc agatcaccaa gccgacggcc ggcgcattgc
    17881 tgttgtcggt ggtcggcacg gcgagcgtgg tggcagtcgg tcgcgacgtt tcgatctcgg
    17941 ccacgggggt catcctcgcg cttgcggccg cgacggcata cgggctctat ctcgtcgtcg
    18001 gtcgaagcgt gcgtaggcag accgattcca tgactgcagc ctgctgggtg gcggtgggtg
    18061 cagcggcggc gtcgttgatg cgcggtgtcg tgggaggcac gcttcagatt ccgaccggtc
    18121 atgtcgtgca gatcgtgggc tacggcgcgg tcaccgcggc cgcattcgcg ctcacattcg
    18181 cggccctggc ccggatcggt gcgacccaga ccgccgtcgt catgacgttg gaggcggccg
    18241 cgacagtgct gctcgccggc ctcgtgttgg gcgaaggcat ttcgtcagtg caggcgcttg
    18301 gcggcgtggc gatccttacg gcagcggcga tcatcgcgca ctcgcaccgt cgcgagcgtg
    18361 cgcaaaatgt acgcggaatg cggcgtgtcg atgtgcagac acgcactctc gcgggtattg
    18421 gggactagcg ccaggtgtag cgggtctttg gacgacccgc cttgccgtag tccgtctggc
    18481 gcgtgactgt ggcttcgtcc gcgagccgtt cgaggtagcg ccaggccgtc acccgggaca
    18541 ccccgacctg cttggccacc tcgtcggcgg tgatgccatc ggcggaatcc cggactgcgc
    18601 gggcaatctc gtcgttggtg ccgggcgccg cacccttggg tgccgcggat ctgtctgcgc
    18661 cgacccgcaa ttcggccagc gcgcggtcga cctccgcctg actggccgcg tccgtgccgg
    18721 cgggtagcgc ctctcggtac cgcttgtagc gctccagccg atcccggaac gccgcgaagg
    18781 taaatggttt gagcagatac gccagcgcgc cgtgccccac ggcggcgcgc accatctcca
    18841 gatcccgttc ggaggttatt gcgatgatgt ccggcgctgg ccgcaggcct gacagccccg
    18901 acgccagcgc gatcccgttc gcatcgggca gtccaatatc cagcagcacc aagtcaattg
    18961 gtgtctcgct cgccatcgct tccgatgccg cgcgcatcgc gtcgcgcgcc gtgtgtgcga
    19021 cggccgccac cgaaaaaccc gacagccgac cgagatacgt gcgatgcgcc tcggcgatca
    19081 gcggttcgtc ctcgacgatc agcacgctga tcacgatttc accgtcactg tcaccacgga
    19141 cccgtaggtc acgtcggcgg tcagcgtgcc gttgtgccgc tggaccacct gcgctaccaa
    19201 cgccaatccc aagccgtgtt gttcagaatc gtcttttgtg gaatagccgc gctgcatggc
    19261 cttttcgaag gttgccgcat ccattcctgg tccgctgtcc gccacccgga tcagcagcgt
    19321 gccgtcgtcc tggctgaccg tcacctcgac ccacgggtct tcgcgatcgc atgcgtccat
    19381 cgcgttgtcg atcaggttgc ccaacaccgt gaccatttcc tggcccgaca gggcgaggtc
    19441 gtcggcgttc gacgggagat gcgtctcctc ggtcacggtc aactcgatgc cgcgttcgtc
    19501 ggcctgcgcc gtcttgccaa gcagcagcgc gaccagcgct ggctcgctaa ccgtgctgga
    19561 gagccggtcg accaattgtt gcgacagctc gagttcgttg gtcgcgaact tgacggcttc
    19621 gtcggcgcgg cccatctcga ccatcgtgac gatggtgtgc agcttgttgg ccgcctcatg
    19681 cgcctgcgag cgcaaggtgt cggtgagcac cttcagcgag ctgagttcgc ccagtgcgcc
    19741 ttgtaattcg gtgcggtcgc ggatcgtcac gacctcagaa tcgcgcgggg cgtcttccac
    19801 ccgagagcgg ttgaccacca gcacccgctc cgcggtgacg tgcacctcgt cgcgtgctcc
    19861 cgggttgtac gtgcgcagga actccggcag atccgatcgg tccaccgggc cgggcggcag
    19921 tgcgagcagc ctgcgggcct cgtcattggc cagcgctaca ccattgcggt ccaacacaat
    19981 cagtccctcc gagaccgaat gcaagatcgc gtcgtggtgt tcgtacatca cccgcagctc
    20041 gtcggggcgt agcccatggg tctgccgtag caggcggcgg cggatcgccc ataccccgac
    20101 cagcgcgatg gccagtgcgg cgaggctcac cgcgacgatc gtcggcacct gcgagcgcca
    20161 gcggtcggcc aggctctgct gcaggatgcc cgcggacacc agcccgacga tgcggccggt
    20221 ctggtcgcgc accggtgcga ccgcgcgtat cgacgggccc agcgtgccgg tgtacacctc
    20281 cgtgaacgtc tcaccctgca gcgccggctc gatggtgccg atgtacttgc cgccgatctg
    20341 gtttgggtcg gtgtgggtga agcgcgtgcg gtcgggcgcc atgatcgtga tgaacgcgat
    20401 gtcggtgttc ttgcggacgg cctcggtgac gggttgcaga gtcgcggtgg cgtttcccga
    20461 ttggatcgcg gcggccgtcg acggtgaatc ggccagcgcg gtggcgatgc cgaggacttg
    20521 ttggcgcgcg gcggcgtcac cgtcgcgggc ggcgtcgaac agcgccagcg cgctgccggc
    20581 cagcaccacc accgcgatca ccaggatctg cagggcgatc gcctgaccgg ccagcgatcg
    20641 tggccagaac ttgttcaatg gcgccttccg ctagacgtac tgaacgccgg actgctgcaa
    20701 ttctggtcac ctttgccgcc gatggagaga aatcacgacg aatgcgtctg tgatgattcc
    20761 gccaatcttg gcggccccgc gccccccgcg gtcgtgctga tcaactcatg gcgtcaaggg
    20821 cggttagctg agctcgcccc gtggtagtta tacacagagg agaaaaccga tgatgcgaga
    20881 ccgttcgagc tatctggtct gcttgtgcgc ggtgtcgctc gccatcgctt cgctgctgct
    20941 gccaacggct tctgccgagc ctgcgtcctg gaacggcgag tacgcgatca cgttcatcgt
    21001 cggtccgaaa gagggaagca gcatcgcagc cggtcagtcc gaggtgcagt acaccgatac
    21061 gtacggcttc agctcgagct gtgacagcgg taagtgcacc gcgacgatca tcagcggccc
    21121 tccgccaagg aacccgacgg ttccgcaacc gattcaattc acgtgggatg ggtcgtcctg
    21181 gacacaagcc accgatttcc agtgggactg catgatgccc gacaccacga ttcaatggag
    21241 tccggcccga ggtgacgtgc gctacacgcc ccaacccgac gggacacttg ccgggaccat
    21301 gcataccgag atcatgagcg gtccgtgcca gggcaccctt gacattcaca tgacggccga
    21361 gcgggtttag gcgcctagcg ccgggccgcc gcgtctttgg tgggtgcgag cggctggcct
    21421 gaacgtactg gatcagtatg ttgagttggt taacccaggc tgtgcgttcg caattcgggc
    21481 cgactgagcc gacccacaca gcaaacacat ggcggccgtg cggcaggggg tgggggcgct
    21541 cttagcgcca cagtggtggc cgaacgccgc cgcgccgagg taatcgggtg ggtgggcaat
    21601 taggtcactg tcaccccgaa cccacacctg aggcgccgaa aaggcgcggg agacccagta
    21661 tgggcgtgac ttcagcccgg ggcctgggag cgttcagcaa gcactcagat gggtacgcat
    21721 caggtagtca ccagcagttt cacaaggcaa ttatgatgat ccgcaactta gcaatgagtg
    21781 gcatttcggt ggttggcgcc ctcgggatcg ctgcgcccgc aaacgccgac cagtatgact
    21841 tcatctctgt gctggataac aacggggtct actactacaa catttcggag atgattgacc
    21901 tcggtaaagc cgcatgtcgg accgttcgca ccggcggcac catcgggtcg gtcgattcgg
    21961 ccatcaacct tctgacccgt gccgggttca acgcggttat ggaccgggca ataatcatgc
    22021 ccgcggcagc gaacaccatg tgcccggata tttggcccgc cctgaatgcg cacaaccaag
    22081 cagcccgcga tctcctgcaa ggcacggccc ccgccaactg aacccatcac tgatcaacta
    22141 atcgaacgcc ggctggcgcg aacttaatga actaaaacgt gacctggctc acctcctggc
    22201 cgaagctcct tgcagaccac attccgcatc tgcttggagg aaacgaccat gaccacgcat
    22261 ctggacaggc cgccggtaga ggagccaccg cgccgccgcg accgcacgca ctggctctac
    22321 atcgccgtca tcgtcgcggt gctggccggc atcgccgtcg gtctggtcgc cccggaagtc
    22381 ggcaagagcg tcggcgtgct cggcacgatg ttcgtcagcc tgatcaagat gatgatcgcc
    22441 ccggtcatct tctgcacgat cgtgctgggc atcggatcgg tgcgcaaagc cgccaccgtc
    22501 ggaaaggtcg gcggcctggc gttcgtctac ttcctggtga tgtcgacctt cgcgctcgcg
    22561 atcggcctcg tcgtcggcaa cctgattcac cccggcagcg ggatgcacct ctccgagact
    22621 gcggcgggca agggcgccga actcgctgaa aaggcgcatg agtccggcgg cctgatggac
    22681 tttgtccagg gcatcattcc ggacacgttg ttctcgtcgc tgaccgcggg cagcgtgctg
    22741 caggcgctgt tcgtcgcgct gctcgtcgga ttcgcgcttc aggccatggg ctcggcgggt
    22801 cagccgatcc tgcgtggcat cgagcatctg cagaagctgg tcttcaaggt gctcgtgatg
    22861 atcctgtggc tggctccgat cggcgcgttc ggcgcgatcg ccaacgtcgt cggccaaacc
    22921 ggttggaccg ccgtgactca gctgctgacg ctgatgctcg ggttctacgt cacctgcgtg
    22981 ctgttcgtgt tcggcgtact gggcgtgctg ctgcgcgtgg tgtcgggcat ctcgatcttc
    23041 aagctggtgc gctacctggc ccgcgagtat ctactgatct tctcgacttc ctcgtcggag
    23101 tcggcgttgc cgcggctgat cgcgaagatg gagcacctgg gtgtcgaccg cagcaccgtc
    23161 ggtgtcgtcg tgccgaccgg ctactcgttc aacctggacg gcaccgcgat ctacctgacc
    23221 atggcgtcgc tgttcatcgc cgacgcgctc ggcagcccga tgtcggtcgg tcagcagatc
    23281 ggtctgctgg tgttcatgat cgtcgcgtcc aagggcgccg cgggtgtcac cggcgccggg
    23341 ctggccacgc tggccggcgg cctgcagagc caccgccccg atctgctcga cggtgtcggc
    23401 ctgatcgtcg gtatcgaccg gttcatgtcc gaagcgcgtg cagtgacgaa cttctccggt
    23461 aacgcggtcg cgacggtgct cgtcggttcg tggaccaaga cgatcgatcg cgaccaggtg
    23521 aacgccgttc tgggtggaga ggacccgttc gacgaactca ccatggtcga cgacgatcgc
    23581 acctcgcacg ccgaagaacc ggcacaggag cgtgtccctg tgccggcgta gggcgcgcga
    23641 acgaaaggcc cggccgaact cccccc
//
LOCUS       DASRJA010000144         3840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2710270,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000144 DASRJA010000000
VERSION     DASRJA010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2710270"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFC01_06710"
     CDS             <1..297
                     /locus_tag="VFC01_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZN79365.1"
                     /translation="PDCTASGFSHTASGVLAAAGAWLDSHQEANNVLTAAGMQGAGAE
                     QSVRDYFVGRPQEYQELRGIAAPLIDLQRGCNTAIQPMQIAALYQALSQSGAPM"
     gene            complement(301..1119)
                     /locus_tag="VFC01_06715"
     CDS             complement(301..1119)
                     /locus_tag="VFC01_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HZN79366.1"
                     /translation="MVDNPLQSAVENATDNDAFEYAARAGFAFSGVLHLVVGYIMVQI
                     AFGSGGEADQSGALGTLARQTGGALILWVVAGGLVALGLWRVAEAIVGSKPGERSNWS
                     ADDTPVWKRGKSLGLAIVNFAIAFSAARFAMGAGKQSSQQNAGLSARMMQTDWGKTIL
                     IAVALGLIAVGGYHVYKGASKRFFKDLRVYGGTGVTAVGVTGYVAKGVVLAGAGVLVI
                     VATLQADPSKATGLDATVKALGNAPFGKVLLVAAGLGIAAFGLYSFVRSRYGRM"
     gene            1247..1615
                     /locus_tag="VFC01_06720"
     CDS             1247..1615
                     /locus_tag="VFC01_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZN79367.1"
                     /translation="MTTLTRAEIGALGEQLAVEHLTSLGLRVLTRNWRCRYGELDVIA
                     ADDAARTVVFVEVKTRTSDQFGGVSQAVTPLKVRRLRRLAGLWLATQRGSWAAVRIDV
                     IGVRIGRTRAPEITHLKAVG"
     gene            1615..3126
                     /locus_tag="VFC01_06725"
     CDS             1615..3126
                     /locus_tag="VFC01_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HZN79368.1"
                     /translation="MALGRAFSVAVRGLDGEIVEIEADISSGLPGVHLVGLPDAALQE
                     SRDRVRAAITNCGHSWPMARLTLALSPATLPKMGSVYDLALACAVLSANGKKAWERLE
                     KTVLLGELALDGRVRPIKGVLPAVLAAKREGWPAVVVPIDNLAEASLVEGIDVWGVRT
                     LGQLHKWLTGAAQLDDRMGTPSSMPEPPMDLADVVGQSHARFAVEVAAAGAHHLMMTG
                     PPGVGKTMLAQRLPGLLPPLSEEESLEVTAIHSVAGLLSGSTPLITRPPFVAPHHTSS
                     VAALVGGGSGLARPGAVSRAHRGVLFLDECAEIGVSAMEALRTPLEEGEIRLARRDGV
                     ACYPARFQLVLAANPCPCAPADPKDCICASMAKRRYLGKLSGPLMDRVDLRVEMHMAR
                     AGAFAAEEGESTAVVRQRVAAARAVAEERWRSHGIRTNAEVSGALLRRKYRPEADAMK
                     PLRTALDSGQISIRGMDRTLRVAWTLADLAGRTSPGVEQVSTALSFRQGGGAR"
     gene            3123..>3840
                     /locus_tag="VFC01_06730"
     CDS             3123..>3840
                     /locus_tag="VFC01_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZN79369.1"
                     /translation="MTDSVMSAYAYLSRVVEPPCRQLAALVTRVGPVEAADRVKRRAV
                     DEQLLRLTEARYEIDSAARDLAVLERLGGRLVTAADTEWPLLAFTAFAGVDTQQRAEA
                     HPPLALWVLGPACLDEVAERAAAIVGTRAATTYGEHVAAELAAGLAERDVAVVSGGAY
                     GIDGAAHRAALAADGFTVAVLAGGIDVSYPAGHAALLHRIGEEGVIVTEYPPGLRPQR
                     HRFLTRNRLVAALAGATVVVE"
BASE COUNT          619 a         1219 c         1366 g          636 t
ORIGIN      
        1 cccgattgca cggcgagcgg cttctcccac acagccagcg gcgtgctcgc agcggcgggc
       61 gcgtggctcg actcgcatca ggaagccaac aacgtattga cggcggcggg catgcagggc
      121 gcgggcgccg agcagtcggt acgcgactac ttcgtcggcc gcccgcagga gtatcaggag
      181 ctgcgcggca tcgcggcgcc gctgatcgac ctgcagcgcg gctgcaacac ggcgatccag
      241 ccgatgcaga tcgccgcgct ctatcaggcg ctgtcgcagt caggcgcgcc gatgtagggg
      301 ctacatccgg ccgtagcgac tacgtacaaa gctgtagaga ccgaacgccg cgatacctag
      361 ccccgccgcg acgaggagga cctttccgaa cggggcattg ccgagtgcct tcaccgtggc
      421 atcgagaccg gtcgccttcg acgggtcggc ctgaagggtc gcgacgatca cgagcacacc
      481 agcgccggcg agcaccacgc ccttggcgac gtaaccggtg acaccgaccg cggtgactcc
      541 cgttccgcca tacacgcgta agtctttgaa gaacctcttg gatgcgcctt tgtacacgtg
      601 atagccgccg accgcgatga ggcccaatgc cactgcaatg agaatggtct tgccccaatc
      661 ggtttgcatc attcgggcgc ttagcccggc gttctgctga ctgctctgtt tgccggctcc
      721 catcgcgaat cgcgcagcgg agaaggcgat cgcgaagttc acaatcgcga gtcccaagga
      781 tttcccgcgc ttccacaccg gggtgtcatc tgcgctccag ttggagcgct cgccgggctt
      841 ggaaccaacg atggcttcgg cgacccgcca taggccgagc gcgaccaacc cgcccgcgac
      901 gacccacagg attagcgcac cgccggtctg gcgcgccagc gtcccaagtg cgccggactg
      961 atcggcttcg ccgccggagc cgaacgcgat ttgcaccatg atgtacccga cgacgagatg
     1021 cagcacacca ctgaacgcga acccggcgcg ggccgcgtac tcgaacgcgt cgttgtcggt
     1081 tgcgttttca actgcgcttt ggagcggatt gtcgaccatg gctccgtata cccgtgtttt
     1141 gacgactgcg aaaccgtgcg cgcggcgatt gtggatgaat gcgcgactgg ggataacaac
     1201 gcattcgggt cagtgctttg tcagtgccgg tccggaccgt ggcgtcatga cgacattgac
     1261 ccgtgccgag atcggcgcac tcggagagca gctggcggtt gagcatctga cgtcgctcgg
     1321 actgcgcgtg ctgacccgca actggcgttg ccgatacggc gagctggacg tgatcgcggc
     1381 cgacgacgcg gcgcgcacgg tggtgttcgt tgaggtgaag acgcgaacca gcgaccaatt
     1441 cggtggcgtg tcccaggcag tgacgccctt gaaggtgcgc cggctgcggc ggctggccgg
     1501 gctgtggctc gcgacgcagc gcggcagctg ggcggcggtg cgcatcgacg tgatcggcgt
     1561 acgcatcggc cgtacccggg ctcccgagat cacgcacctg aaagcagtcg gctgatggcg
     1621 ctgggcaggg cgttctcggt ggcggtgcgt ggcctcgacg gtgagatcgt ggagatcgag
     1681 gccgatatca gctccggtct gcctggcgtg catctggtcg ggctgcccga cgccgccctg
     1741 caggagtcgc gggaccgggt gcgcgcggcg atcaccaact gcggccacag ctggccgatg
     1801 gcgaggctga ctttggcgct ctccccggcg acgcttccga agatgggttc ggtctacgat
     1861 ttggcgcttg cctgcgcggt gttgtcggcc aatggcaaga aagcgtggga gcgactcgaa
     1921 aagaccgtgc tgctaggaga attggcgcta gacgggcggg tccgtccgat caagggggtg
     1981 ctgcccgccg tactggcagc caagcgtgaa ggctggcctg cagttgtggt ccccatcgac
     2041 aacctcgcgg aggccagcct ggtcgagggt atcgatgtgt ggggcgtacg cacactgggg
     2101 cagctgcaca agtggctgac cggagccgct cagcttgacg accggatggg cactccatcg
     2161 tcgatgccgg agccgcccat ggacctcgcc gatgtggtcg gccaatcgca cgctcggttc
     2221 gcggtcgagg tcgccgcggc cggagcccat cacctgatga tgaccggccc gccaggtgtc
     2281 ggtaaaacga tgctggcgca acgccttccg gggctactac cgccgctgtc ggaagaggaa
     2341 tcgctggagg tgacggcgat ccactccgtg gcaggcctgc tgtcgggcag cacgccgctg
     2401 atcacccggc cgccgtttgt cgccccgcac cacacgtcga gtgtggccgc attggtcggc
     2461 ggcggctcgg gcctggcccg accgggagcg gtcagtcgcg cgcatcgggg cgtgttgttc
     2521 ctcgatgagt gtgcggagat cggggtcagt gcgatggaag cactgcgaac tccgttggag
     2581 gagggcgaga ttcggcttgc ccgccgcgac ggcgtcgcct gttatccggc acgattccag
     2641 ttggtgctgg ccgccaaccc gtgcccgtgc gcgccagcgg acccgaagga ctgcatctgc
     2701 gcgtcgatgg ccaagcggcg atacctcggc aagctctccg gaccactaat ggatcgcgtg
     2761 gacttgcgcg tggaaatgca catggcccgg gcgggagcct tcgccgcgga agaaggcgag
     2821 tcgacggccg tcgtcaggca acgggtggcg gccgcgcgtg ctgtcgccga agaacgttgg
     2881 cggtcacatg gcatacgcac caacgccgag gtcagcggtg cactgctgcg gcgcaagtac
     2941 cggcctgagg cggacgcgat gaagccgctg cgaaccgcgc tcgacagcgg ccagatcagc
     3001 attcgaggca tggatcgcac gttgcgggtc gcgtggacgc tggccgatct cgccggtcgg
     3061 acctcaccgg gtgtcgagca ggtcagcacg gcgctgagtt tccgtcaggg tgggggcgcg
     3121 cgatgaccga ttccgtgatg tcggcctacg cttacttgtc gcgggtcgtc gaaccgccct
     3181 gccgacaatt ggcggcgctg gtcaccaggg tgggaccggt cgaagccgcc gaccgggtga
     3241 agcgaagggc cgtcgatgag cagctgctgc gattaaccga ggctcgctat gaaatcgact
     3301 ctgctgcaag ggatcttgcc gtattggagc gactgggtgg acgactggtc actgcggcgg
     3361 acaccgaatg gccactgctg gcgttcaccg ctttcgcagg tgtcgacacg cagcagcggg
     3421 cggaggcgca tcccccgctt gcgctgtggg tgttggggcc ggcgtgcctc gacgaggtgg
     3481 ccgagcgggc ggcggcgatc gtcggaaccc gggcggccac aacctacggc gagcatgtag
     3541 ccgcggagct ggccgccggt ctggcggagc gggacgtggc cgtggtgtcc gggggtgcct
     3601 acggcatcga cggagcagca caccgtgccg cgctggcggc cgacgggttc acggtcgcgg
     3661 tattggccgg cggcatcgat gtctcttacc cggcgggcca cgccgcgctc ttgcatcgga
     3721 ttggcgagga aggcgtgatc gtcaccgaat atccgccggg cctgcggccc cagcggcacc
     3781 ggttcctcac ccgcaaccgg ctcgtcgctg cgcttgcggg cgccaccgtg gtcgtggaag
//
LOCUS       DASRJA010000145         6242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3876629,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000145 DASRJA010000000
VERSION     DASRJA010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3876629"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFC01_06735"
     CDS             <1..418
                     /locus_tag="VFC01_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HZN79370.1"
                     /translation="ATVTVDGWLKTGDAGYVDDAGFIYLHDRLKDMIVSGGENVSPAE
                     VENVLMTHPGVGDVAVIGVPDERWGEAVKAIVVPTAGTTPSEAELIAYARERLAGFKL
                     PKSVDFADALPRTPSGKLLKRTLREPYWEGVGRQIG"
     gene            441..812
                     /locus_tag="VFC01_06740"
     CDS             441..812
                     /locus_tag="VFC01_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN79371.1"
                     /translation="MEILASRMLIRPADYERSRRFYRDELGLAIFRDYGAGTVFFAGQ
                     SLIELAGHGTPSAGGTMWLQVRDVYATEQELKGRSVEIAREAKQEPWGLHEMHVVDPD
                     GVLLIFVQVPETHPLRRDTRG"
     gene            complement(822..1127)
                     /locus_tag="VFC01_06745"
     CDS             complement(822..1127)
                     /locus_tag="VFC01_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HZN79372.1"
                     /translation="MTENQTVLDNVLGWLHQGYPEGVPPKDYFALLALLKRSLTEEEV
                     VKAAQSILKSNNSDTVTEDEIRDAVHAVIEKEPNPEEIHQVAARLASVGWPLASPAS"
     gene            complement(1139..1423)
                     /locus_tag="VFC01_06750"
     CDS             complement(1139..1423)
                     /locus_tag="VFC01_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79373.1"
                     /translation="MTYAESILKVLVVGLLLGAGLPAVFAGGLVAYSSGAGGQASDGT
                     VHAPNPVLKYLGIALFVFVAWVIVTAVFWITRTTIIHHFGVDLFPFMPKK"
     gene            complement(1453..2700)
                     /locus_tag="VFC01_06755"
     CDS             complement(1453..2700)
                     /locus_tag="VFC01_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HZN79374.1"
                     /translation="MSAEMIILVLLIATALAFDFTNGFHDTGNAMATSIATGALKPKT
                     AVLLAGVLNLVGAFLSVEVAVTVTTSVLKVQDSKTGHLLPSIDASTGLTIIFAGLIGG
                     ILWNLLTWLFGIPSSSSHALFGGLIGAGLAALGTAGVNWSGITQKVLIPAVAAPVIAC
                     LVAACGTWLVYRITRKVLQKRREEGFRWGQIATASLVALSHGTNDAQKTMGVIALALI
                     TTGHLTGDVKKDGLPFWIIFSCALAIGLGTYIGGWRVIRTLGKGLVEIESPQGFAAEA
                     SSAAIILSSSAAGMALSTTHVATGSILGSGVGKPGAEVRWAVAGRMAVAWLVTLPAAG
                     IVGALSYWLSDAVKSVSGSALAGDGLIFLILVGLSFYMWWRAQQQKVDHKNVNADWDD
                     STNSVVPAEVREAAKAPKDTAAV"
     gene            2917..3219
                     /locus_tag="VFC01_06760"
     CDS             2917..3219
                     /locus_tag="VFC01_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HZN79375.1"
                     /translation="MTKFLAKIVAWITSGYPEGVPGPDRVPLFALLRPRLTDDEVKTV
                     AKALMDRGEFDHVDIGVLITQMTDELPAPEDVERVRNRLAAKGWPLDEPRDAEETE"
     gene            3177..4346
                     /gene="menE"
                     /locus_tag="VFC01_06765"
     CDS             3177..4346
                     /gene="menE"
                     /locus_tag="VFC01_06765"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HZN79376.1"
                     /translation="MAARRAARRRGDRIAVLRPVAVPSGASALSLLPVLEDALAGGAS
                     VLPVPADDARQSALLTTSLRAGLAIDDDVAVVVSTSGTTGTPKGAMLTASALTASAEA
                     THRRLGGPGAWLLALPAYHVAGLQVLVRSVVAGTTPIACFDVAEFPSAVTSLGGGRRY
                     ASLVAAQLDKALRDSAATDALASLDAVLIGGGPMPPGLAERASAVGVSVVRTYGMSET
                     AGGCVYDGVPLGGVRVRVDDGRVVLGGATLAKGYRNPVQPDPFAEPGWFRTDDIGTVD
                     DSGVLRVLGRIDDAISTGGLTVLPHLVEAALATHPAVAESAVFGVADDRLGQRVVAAI
                     VVSPGCATPTLTDLREHVTSTLSSTAAPREVHLVDELPRRGIGKVDRRELAARFG"
     gene            4420..4704
                     /locus_tag="VFC01_06770"
     CDS             4420..4704
                     /locus_tag="VFC01_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005081381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79377.1"
                     /translation="MNNFVITPLAAVALGAAALGLAATATAFPNAGTAEDVVDSLKAE
                     GYNVQVNGLVQVPLKLCTATDVHPTLDESDSLQDKQHTMVFVDVSCPSHD"
     gene            4719..5237
                     /locus_tag="VFC01_06775"
     CDS             4719..5237
                     /locus_tag="VFC01_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79378.1"
                     /translation="MLRALALIACVVSLFGGPATASADPAGQGDAVFAIGKCFDPSQP
                     PQQRPGSLAYNCDTTGVMENMTWTSWGPDGARGTGTDSAVECKPNCAEGPRLFNPIVV
                     HAWNASAPTSPACPSGVQFYSDLTIAYPQGVPPWIAPGTTWSPGTDFVTVDGMPAVHF
                     SGLTPTCAPPGI"
     gene            complement(5234..>6242)
                     /locus_tag="VFC01_06780"
     CDS             complement(5234..>6242)
                     /locus_tag="VFC01_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HZN79379.1"
                     /translation="GLRELLDDSVGDGWLADLDKLRGLESYADDAAFRLQWREVKRAN
                     KARLADYVKATAGVELNPDWLFDIQVKRIHEYKRQHLNMLHIVALYHRIKQNPNLDIP
                     SRAFIFGGKAAPGYFLAKRIIKLINAVGETINNDPDVNRFVKVAFVPNFNVQNAHLIY
                     PAADLSEQISTAGKEASGTGNMKFMLNGALTIGTLDGANVEMREEAGAENFFLFGLAV
                     EEVERIKRDGYRPSDYVNGELAAVLDLIAGGAFSDGDTEVFRPLVDNLRSDDPFLVLA
                     DYASYVEAQERVSDAWRDIPAWTRMSILNTARSGKFSSDRAIAEYWDEIWNVLPVTVK
                     R"
BASE COUNT         1063 a         2003 c         2070 g         1106 t
ORIGIN      
        1 cgcgaccgtc accgtcgacg ggtggctgaa gaccggtgac gcgggctacg tcgacgacgc
       61 gggcttcatc tatcttcacg accggctgaa ggacatgatc gtctcgggtg gcgagaacgt
      121 gtcgcctgcc gaggtcgaga acgttctgat gacgcatccc ggggtcggcg atgtggctgt
      181 catcggcgtg cccgatgagc ggtggggcga ggcggtcaag gccatcgtcg tgcccacggc
      241 cgggaccacg ccgagcgaag cggagctgat cgcttatgca cgcgagcggc ttgcggggtt
      301 caagctgccc aaatcggtgg acttcgccga cgcgttgccg cggaccccga gcggcaagtt
      361 gctcaagcga acgctccgcg aaccctactg ggagggtgtc gggcgtcaga tcggctgacc
      421 atcgtgatag acacggatgc atggagatcc tggccagccg gatgctcatc cggccggcgg
      481 actacgaacg gtcgcggcgg ttctaccgcg acgagctcgg gctcgcgatc tttcgcgact
      541 acggcgcggg cacagtgttt ttcgccggcc aatcgctgat cgaactcgcc ggccacggca
      601 cgccgagtgc cggtggcacc atgtggcttc aggtccgcga cgtgtacgcc accgaacagg
      661 agctcaaggg ccgcagcgtc gagatcgctc gcgaggcaaa gcaggaaccg tggggcctac
      721 acgagatgca cgtcgtcgat cccgacggtg tcctgctgat cttcgtacag gtacccgaaa
      781 cccacccgct gcgacgcgat accagaggct gacggctccg atcagctggc aggcgacgcc
      841 aacggccacc cgacggaggc caaccgcgcg gcgacctgat ggatctcctc cgggttgggc
      901 tccttctcga tcaccgcgtg cactgcgtcg cggatctcgt cctcggtcac cgtgtcggaa
      961 ttgttcgact tgaggatgga ctgggccgcc ttgacgacct cctcctccgt cagcgaccgc
     1021 ttcagcagcg cgagcagcgc gaagtaatcc ttcggcggaa caccttccgg gtagccctga
     1081 tgcagccacc caagcacgtt gtccaggaca gtctgattct cggtcatgtg gtctcagctc
     1141 acttcttggg catgaagggg aacaggtcaa caccaaagtg gtggatgatc gtcgtcctgg
     1201 tgatccagaa gaccgcggtg acgatcaccc atgcgacgaa tacgaacagc gcgatgccga
     1261 ggtacttcag cacgggattg ggtgcgtgca cggtgccgtc tgacgcttgg ccgcccgctc
     1321 cgctcgaata cgccaccagt ccgccggcga agaccgcggg gaggcctgcg ccgaggagca
     1381 gccccacgac gagcaccttc aaaatgcttt ctgcgtaagt catcaacgtt tcctttgctt
     1441 gtcttcgaag gcctagacgg ccgccgtgtc cttgggcgcc ttggcggcct cgcggacttc
     1501 ggccggaacg acggagttgg tcgagtcgtc ccaatcggcg ttgacgttct tgtggtccac
     1561 cttctgctgc tgggcacgcc accacatgta aaacgacagt ccgacgagga tgaggaagat
     1621 cagtccatcg cccgccagcg ccgaaccgct gacagacttg acggcgtccg agagccagta
     1681 cgaaagcgcc ccaacgattc cggcggcagg cagggtgacc agccacgcca ccgccatgcg
     1741 gccggcgacc gcccagcgca cctcggcgcc cggcttgccg acgccactac ccaagatcga
     1801 gccggtcgcg acgtgggtgg tcgacagcgc catgcctgcg gcgctcgagc tcaaaatgat
     1861 tgcggccgat gatgcctctg cggcgaagcc ctgcggcgac tcgatttcga caaggccctt
     1921 gcccagggtg cggatgacgc gccagccacc gatgtaggtg ccgaggccga tcgcgagcgc
     1981 gcagctgaag atgatccaga acggcaggcc gtccttcttc acgtcgccag tcaggtgacc
     2041 ggttgtgatc agtgccagcg caatgacgcc catcgtcttc tgggcgtcat tcgtgccgtg
     2101 cgacaacgca accagcgagg cggtcgcgat ctggccccaa cggaagcctt cttcacgtcg
     2161 cttctgcagc accttgcgcg tgatccggta gaccagccag gtaccgcatg cggcgacgag
     2221 gcaggcgatc accggggcgg cgaccgcggg aatcagcacc ttctgggtga tccccgacca
     2281 gttgacaccg gcggtgccca gcgcggccag gccggcgccg atcaggccgc cgaagagcgc
     2341 gtgcgacgaa ctggatggga tgccgaacag ccaggtgagc aggttccaca gaatgccgcc
     2401 gatgaggccg gcgaagatga tcgtcagacc cgtcgacgca tcgatcgacg gaagtaggtg
     2461 gccggtcttg ctgtcttgga ccttgagcac cgacgtggtg acggtgacgg cgacctcaac
     2521 cgagaggaag gcgccgacga ggttcagcac gcccgccaac agaacggcgg tctttggctt
     2581 gagggcaccg gtggcgatcg aggtcgccat cgcattgccg gtgtcgtgga atccgttggt
     2641 gaagtcgaag gccagtgctg ttgcgatcag cagcaccagg atgatcatct ctgcgctcat
     2701 ggctgctaat tctggggcca gagcggcaaa ttagaccagc cgctagctac cctaaacggc
     2761 cgctccgacc tgcgactttg ccttgtccag tgaggtgttc atacagtgtt cactcggcgg
     2821 tccggaaccg tttgtctgct ccgtcggcgg ggccgggcac taccatcgat ctgtccaggg
     2881 aaccgaggcc accggcctcc gtcgggagtt gacgccgtga ccaaatttct cgcgaagatc
     2941 gtcgcgtgga tcacctcggg gtatccggaa ggcgtgccgg gtccggatcg ggttccgctg
     3001 ttcgcgctcc tgcggccgcg gctcaccgac gacgaggtga aaaccgtcgc gaaggcgttg
     3061 atggaccgcg gcgagttcga ccacgtggac atcggcgtgc tgatcaccca gatgactgac
     3121 gaattgccag ccccggagga cgtcgagcgg gtacgaaacc ggttggcggc caagggatgg
     3181 ccgctcgacg agccgcgcga cgccgaggag accgaatagc cgtcctgcgc cccgtcgctg
     3241 tcccgtcagg cgcttcagcg ctgtcgctgc tgccggtgct ggaggatgcg ctggccggag
     3301 gcgcgtctgt ccttcccgtg ccggccgacg acgcgcgtca aagtgctttg ttgacaacgt
     3361 ctttgcgcgc aggcttggcg atcgatgacg acgtcgctgt ggtggtgtcg acgtcgggga
     3421 cgacgggtac acccaagggg gcgatgctca cggcgtcggc gttgacggcg agtgcggaag
     3481 ccacgcaccg tcggctgggc ggtcccgggg catggctgct cgcactgccg gcctatcacg
     3541 tcgcggggct gcaggtgttg gtgcgcagcg tcgtcgcggg tacgacgccc attgcctgtt
     3601 tcgacgtagc cgaattcccc tccgccgtca cctcattggg tggcggacgc cggtatgcgt
     3661 ccctggtggc ggcgcagctg gacaaggcgt tgcgtgactc tgccgcaacc gatgctctgg
     3721 catcgctgga cgcggtgttg atcggcggcg ggccaatgcc accgggattg gccgaaagag
     3781 cctcggcagt aggggtttcg gtcgtccgca catacggcat gagcgagaca gcgggcggtt
     3841 gcgtgtacga cggcgtgccg ttgggcgggg tgcgggtgcg cgtcgatgac ggccgggtgg
     3901 tgttgggcgg cgcgacgctg gcgaagggtt accgcaatcc ggtgcaaccg gatccgtttg
     3961 cagagccagg ttggtttcgc accgatgaca ttggcaccgt tgatgattcg ggtgtgttgc
     4021 gggtgctagg ccggatcgac gacgcgatca gcaccggcgg attgacggtg ttgccgcatt
     4081 tggtggaggc ggcgttggcg acccaccccg cggtggccga gtccgcggtg ttcggcgtcg
     4141 ccgacgaccg actgggccag cgggtagtcg cagcaatcgt cgtctccccg ggctgcgcga
     4201 caccgacgtt gaccgacctg cgcgagcatg tcacgtcgac gctgtcgtcg actgctgcgc
     4261 cacgtgaagt ccatctggtc gacgagctgc cccgccgcgg catcggaaag gtggataggc
     4321 gcgagctggc cgcgcgattc ggttagccgc cttcaatctc caccgcatct ccacacaatc
     4381 tccaagcttc gaagccgctg gccagacagc atttgcgtca tgaacaactt cgtcatcacc
     4441 ccactcgcgg cagtggcgct cggggccgca gccctgggtc tggccgcgac ggccaccgcg
     4501 ttcccgaacg caggcaccgc cgaggacgtc gtcgacagcc tgaaagccga gggctacaac
     4561 gtgcaggtca acggcctcgt ccaggtgcct ctgaagctgt gcaccgcgac cgatgtccac
     4621 cccactctcg acgagtcgga ttcgctgcag gacaagcagc acacgatggt cttcgtcgat
     4681 gtgtcgtgcc cgtcacacga ctgagctaac atccgggcgt gctccgcgcc ctcgctctga
     4741 tagcttgcgt cgtttccctg ttcggcggcc ccgccaccgc atcggcggac cctgccggac
     4801 agggcgatgc ggttttcgcc atcggcaagt gcttcgatcc aagtcaacct ccgcaacaac
     4861 ggcctggctc gctcgcctac aactgcgaca ccaccggcgt catggagaac atgacgtgga
     4921 cgtcctgggg acccgacggc gcgcgtggga cgggcaccga cagcgccgtc gagtgcaagc
     4981 caaactgcgc ggagggaccc cggctgttca atccgatcgt cgtgcacgcg tggaatgctt
     5041 cagcgccaac gagtcccgcg tgcccgtcgg gcgtccagtt ctactccgac ttgacgatcg
     5101 cctatcccca gggtgttccg ccgtggatag cgccgggcac gacctggtct cccggaacgg
     5161 atttcgtcac cgtggacggc atgccggcgg ttcacttctc cggactgacg ccaacctgtg
     5221 cgccccccgg catctagcgc ttgacggtca ccggcaggac gttccagatc tcgtcccagt
     5281 actcagcgat ggcccggtcc gacgagaact tgccgctgcg cgcggtgttc aggatcgaca
     5341 tccgcgtcca ggccgggatg tcgcgccacg cgtcgctcac tcgttcctgg gcctcgacgt
     5401 aggacgcgta gtcggcgagg accaggaacg gatcgtcgga gcgcaggttg tcgaccagcg
     5461 ggcggaacac ctcggtgtcg ccatccgaga acgccccgcc ggcgatcaag tcgaggacgg
     5521 ctgccagctc gccgttgacg taatccgacg gtcggtagcc gtcgcgcttg atccgctcga
     5581 cctcctcgac ggccagcccg aacaggaaga agttctcggc tccggcctcc tcgcgcatct
     5641 ccacattcgc gccgtcgagc gtgccgatgg tcagcgcgcc gttgagcatg aacttcatgt
     5701 tgccggtacc cgacgcttcc ttgcccgccg tcgagatctg ttccgacaga tcggctgccg
     5761 ggtagatcag gtgtgcgttt tgcacgttga agttgggcac gaacgcgacc ttgacgaatc
     5821 ggttgacgtc cggatcgttg ttgatcgtct cgccaacggc gttgatcagc ttgatgattc
     5881 gcttggccag gaaatagccg ggcgccgcct tgccgccgaa aatgaatgcc cgcgagggga
     5941 tatcgagatt gggattctgc ttgatccggt gatacagcgc gacgatgtgc aacatgttga
     6001 ggtgttggcg cttgtattcg tggatgcgct tgacctggat gtcgaacagc cagtcggggt
     6061 tgagttcaac accggccgtc gctttcacat agtcggccag ccgtgccttg ttggcccgct
     6121 tgacctcacg ccactggagg cggaacgcgg cgtcgtcggc ataggactcc aggccgcgaa
     6181 gcttgtccag gtctgccagc cagccgtcgc cgaccgaatc gtcgagcagc tcccgcagcc
     6241 cc
//
LOCUS       DASRJA010000146         9164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1580566,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000146 DASRJA010000000
VERSION     DASRJA010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580566"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFC01_06785"
     CDS             complement(<1..364)
                     /locus_tag="VFC01_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZN79380.1"
                     /translation="MTAVTSAAPSSQLRSRIRQVTGRAVSTLLKLPPHTTDYRVIRGL
                     RTPMRDGVELIADHYEPQTSSPAGTLLVRAPYGRGFPFSIQFAAVYASRGYHVIIQSV
                     RGTFGSGGEFNPMVNEIAD"
     gene            477..842
                     /locus_tag="VFC01_06790"
     CDS             477..842
                     /locus_tag="VFC01_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79381.1"
                     /translation="MKRLVVGLATTVLLSGSVAGVFPGAASALPGDPIPLRSWPGCPE
                     DNPSGPCHWCPGDPPVQTGNLRTNPVVWDNNVCHTYWYVYFGQGNVAQNIFEGEAPPP
                     PPPPPPPGLNFCPIPPWCP"
     gene            complement(846..1415)
                     /locus_tag="VFC01_06795"
     CDS             complement(846..1415)
                     /locus_tag="VFC01_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HZN79382.1"
                     /translation="MVAKLRVLSALCALVAAVIVVWQSNPAGPVQVDATDIPMTNVTT
                     SIKWPVIDTTDPSPFDPCREIPLDVIQRIGLAATPPTPEEGLRCHYDAGNYQMAVEPF
                     VWRSYEEALPPDAVELDINGHRAAQYWIMKPTDWNDRWWITCMITFKTSYGVIQQSLF
                     YSPIYAADDVDCLQANLQRARELSPYYIY"
     gene            1545..2495
                     /locus_tag="VFC01_06800"
     CDS             1545..2495
                     /locus_tag="VFC01_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZN79383.1"
                     /translation="MTRDGRRPTLWAISDLHTGHTGNKPITESLHPSSPDDWLIVAGD
                     VAERTDEIHWSLDLLRKRFAKVIWVPGNHELWTTNHDPMQIFGKARYDYLVNMCDEMG
                     IVTPEHPFPVWNEEGGPATIVPMFILYDYTFLPQGAGTKAEGLAIARERNVVGTDEFL
                     LSAEPYATRDAWCRDRVSYTRKRLEDLDWMDQTVLVNHFPLVREPCDAMFYPEFSLWC
                     GTTATADWHTRYNAVCSVYGHLHIPRTTWYDGVRFEEVSVGYPREWRRRKPYRWLRQI
                     LPDPNYAPGYLNEFGGHFQITQEMREHAEKVQERIRARQS"
     gene            2492..3166
                     /locus_tag="VFC01_06805"
     CDS             2492..3166
                     /locus_tag="VFC01_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HZN79384.1"
                     /translation="MTTAPLLGGVLPGVAALAAAETYDDPPGLAPLPEEEPLIAKSVA
                     KRRNEFITVRYCARLALEELGMPPVPILKGNKGEPCWPDGIVGSLTHCTGFRGAAVGK
                     LGTVRSIGLDAEPHDVLPKGVLDAISLPAERTELQAMPDGLHWDRILFCAKEATYKAW
                     FPLTHRWLGFEDAHITFDVDDSGTTGGFVSRILIDPAAESGPPLQTLSGRWSVRDGLA
                     LTAIVL"
     gene            3163..4044
                     /gene="truB"
                     /locus_tag="VFC01_06810"
     CDS             3163..4044
                     /gene="truB"
                     /locus_tag="VFC01_06810"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZN79385.1"
                     /translation="MTDAGLVVVDKPAGVTSHDVVGRCRRIFGTRKVGHAGTLDPMAT
                     GVLVVGIERATKILGLLTATDKSYAATIRLGQTTSTDDAEGELLQSVSADAVTDTQVD
                     EAVAALRGEIAQVPSAVSAIKVGGKRAYKLAREGQTVELAPRMVRIDRFDVRSIRHHD
                     GLIDLNVEVDCSSGTYIRALARDIGAALGVGGHLTALRRTRVGRFGLDQARILDDLAE
                     SPRLSYTLDEACLQSFPRRDLTADETEDTRHGRPLKPAGFDGVYAATAPDGQVIALLT
                     DGPNRTTSVVVLRPATL"
     gene            4099..4512
                     /locus_tag="VFC01_06815"
     CDS             4099..4512
                     /locus_tag="VFC01_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6632 domain-containing protein"
                     /protein_id="HZN79386.1"
                     /translation="MGSPYKLLQLALAVFGAIMILLYPLALVWPSGWAWHHGAPHHSV
                     YFMMIVGIYATLGAFLLNAARNPAANLSLIWFAVWSSVVHAAIMAVQSFTEHQMGHLW
                     GDVAALLLVAIVLSALVLSAGLKQPAPDRAGTVAR"
     gene            complement(4509..5204)
                     /gene="mntR"
                     /locus_tag="VFC01_06820"
     CDS             complement(4509..5204)
                     /gene="mntR"
                     /locus_tag="VFC01_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HZN79387.1"
                     /translation="MSPDGNPRDLTTVAQDYLKVIWTAQEWSREKVSTKLLAERIGVS
                     ASTASESIRKLADQGLVDHEKYGAVTLTEAGRRAALAMVRRHRLMETFLVRELGYSWD
                     EVHDEAEVLEHAVSDRMLDRIDAKLGHPTRDPHGDPIPAADGRVPTPDARQLSVCQNG
                     DAGTVARISDADPEMLRYFDSVGISLDSRLRVLARRDFAGMISVAIEGAEGSDDTTVD
                     LGSPAAEAIWVVG"
     gene            5286..6260
                     /locus_tag="VFC01_06825"
     CDS             5286..6260
                     /locus_tag="VFC01_06825"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZN79388.1"
                     /translation="MQRWRGQEDIPTDWGRCVLTIGVFDGVHRGHQELIGHAVKAARA
                     RGVPTVLMTFDPHPMEVVFPGSHPAQLTTLTRRAELVEELGIDVFLVMPFTTDFMKLT
                     PDRYIHELLVEHLHVVEVVVGENFTFGKKAAGNVDTLRKAGERFGFAVQGMSLVSEHH
                     QNEDITFSSTYIRSCVDAGDVVAATEALGRPHRVEGVVVRGEGRGMVLGFPTANVAPP
                     MYSAIPADGVYAAWFTVLGHGPITGTVVPGERYQAAVSVGTNPTFSGRTRTVEAFVLD
                     TSADLYGQHVAVDFVARIRGQEKFERIEELVATMGDDTEHARTILSAG"
     gene            6360..6629
                     /gene="rpsO"
                     /locus_tag="VFC01_06830"
     CDS             6360..6629
                     /gene="rpsO"
                     /locus_tag="VFC01_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZN79389.1"
                     /translation="MALTAEQKKDILGQYGLHDTDTGSPEAQVALLTKRIADLTEHLK
                     VHKHDHHSRRGLLLLVGRRRRLLKYVAQVDVERYRSLIDRLGLRR"
     gene            6937..>9164
                     /locus_tag="VFC01_06835"
     CDS             6937..>9164
                     /locus_tag="VFC01_06835"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZN79390.1"
                     /translation="MSVVEIEDGVYESTAVIDNGSFGTRTIRFETGRLAQQAAGSVVA
                     YLDDETMLLSATTASKNPKDHFDFFPLTIDVEERMYAAGRIPGSFFRREGRPSTDAIL
                     TCRLTDRPLRPSFVSGLRNEIQVVVTVMSLDPNDLYDVVAINAASASTQLAGLPFSGP
                     VGAVRVALIDGTWVAFPTVEQLERAVFDMVVAGRALEDNDVAIMMVEAEATENVIELI
                     AGGAQAPTEAVVAEGLEAAKPFIAALCTAQQELAGRAAKPTGEYPVFPDYGDDVYNSV
                     ASVATDELSKALTIAGKHERDDRTDEIKAEVLERLAEQFAGREKEIGAAYRSLTKKLV
                     RQRILTDHFRIDGRGITDIRALSAEVAVVPRAHGSALFERGETQILGVTTLDMVKMAQ
                     QIDSLGPETTKRYMHHYNFPPYSTGETGRVGSPKRREIGHGALAERALVPVLPSIEEF
                     PYAIRQVSEALGSNGSTSMGSVCASTLSLLNAGVPLKAPVAGIAMGLVSDDVEVEGGG
                     TERRFVTLTDILGAEDAFGDMDFKCAGTKDFVTALQLDTKLDGIPSQVLAGALAQAKD
                     ARLTILEVMAEAIDSPDEMSPYAPRITTIKVPVDKIGEVIGPKGKMINSITEETGAQI
                     SIEDDGTVFVGASNGEAAQAAIDKINAIANPQLPKIGERFLGTVVKTTDFGAFVSLLP
                     GRDGLVHISKLGRGKRIAKVEDVVKVGDKLRVEIADIDNRGKISLVLVEEEESAPAEA
                     PAA"
BASE COUNT         1596 a         3020 c         2998 g         1550 t
ORIGIN      
        1 cgtcggcgat ttcgttgacc atcgggttga actcaccgcc ggacccgaat gtgccgcgca
       61 cactttggat gatcacgtgg tagccccgcg acgcatacac cgcggcgaac tggatcgaga
      121 acggaaaccc gcggccataa ggggcacgga ccaagagcgt gcccgcggga ctggaggtct
      181 gcggctcgta gtggtcggcg atcagttcga cgccgtcgcg catcggcgtg cgcaagcctc
      241 ggatcacccg gtagtccgtg gtgtgtggcg gcagcttcag tagcgtgctg acggcgcggc
      301 cggtgacctg cctgattctg gagcgaagct gtgacgaggg cgcggcggaa gttactgcgg
      361 tcacctaaca aagattacgc aagctcgtca tcgatggcag cgaggaccta cggagccgaa
      421 aattggagta gtcaactacc ccagaccagc gtgcttcccg gatcgacgat gtccgtatga
      481 aacggctagt tgtgggcctc gcaacgacag tgctgctctc gggcagcgtc gcgggggtgt
      541 tccccggtgc tgccagcgcc cttcccggtg acccgatccc actacgtagc tggccgggct
      601 gtccggagga caacccatcg ggcccgtgtc actggtgccc gggagatccg cccgtgcaga
      661 cgggcaatct gcggaccaac ccggtggtgt gggacaacaa cgtctgccac acctactggt
      721 acgtctattt cggccagggc aacgtcgcgc agaacatttt tgaaggcgaa gcgccaccgc
      781 cgccgcctcc accgccgccc ccgggcctca acttctgccc gatcccgccg tggtgtccgt
      841 gacggttagt agatgtagta cggcgaaagc tcgcgcgcgc gttgcagatt cgcctgtagg
      901 cagtcgacgt cgtcggcagc gtagatgggc gagtagaaca gcgactgctg aatcacgccg
      961 tagctggtct tgaacgtaat catgcaggtg atccaccaac ggtcgttcca gtccgtcggc
     1021 ttcatgatcc agtactgggc cgcgcggtga ccgttgatat cgagctcgac ggcatcgggt
     1081 ggcagcgcct cctcgtagga gcgccacacg aagggctcga cggccatctg atagttaccg
     1141 gcgtcgtagt ggcagcgcag gccctcttcg ggcgtcggcg gtgtggctgc cagcccgatg
     1201 cgctggatga cgtcgagcgg gatttcccgg caggggtcga acgggctcgg atcggtcgta
     1261 tcgatcaccg gccacttgat ggacgtggtg acgttggtca tcgggatgtc ggtcgcatca
     1321 acctgcaccg gtccggccgg gttggactgc cagaccacga tcaccgccgc gaccagcgcg
     1381 cacagcgccg acaggacgcg cagtttggcg accatttcac cccttacgct cgtccgaccc
     1441 tccggggagt gtatcggtct gccactccca cccggacggg aaacgagaac atgttctagt
     1501 tgccggtcca ggggccgacg atatcgcaag taggctggtc ggttgtgaca cgagacggcc
     1561 gccgacccac gctgtgggcg atcagcgacc ttcacaccgg tcacaccggc aacaagccga
     1621 tcaccgaatc gctgcatccg tcctcgcccg atgactggct catcgtggcg ggggacgtcg
     1681 ccgaacggac cgacgagatc cactggtcgc tggacctcct gcgcaaacgg ttcgcgaagg
     1741 tgatctgggt gccgggcaac catgagttgt ggacgacgaa ccacgatccg atgcagatct
     1801 tcggcaaggc gcgctacgac tacctggtca acatgtgcga cgagatgggc atcgtcacgc
     1861 cggagcaccc gtttccggtg tggaacgagg agggcggccc ggccacgatc gtgccgatgt
     1921 tcattctgta cgactacaca tttctaccgc agggagcagg cacgaaggcc gagggtctgg
     1981 ccatcgcgcg tgaacgcaac gtggtcggca ccgacgagtt cctgctgtcg gccgagcctt
     2041 acgccacccg cgacgcgtgg tgccgagacc gggtgtctta cacgcgcaag cgcctcgagg
     2101 acctcgactg gatggaccag accgtgctgg tgaaccactt cccgctggtc cgcgaaccct
     2161 gcgatgcgat gttctatccc gagttctcgc tgtggtgcgg caccacggcg accgccgact
     2221 ggcacacccg ctacaacgcg gtctgttcgg tctacggcca tctgcacatc ccgcgcacca
     2281 cgtggtatga cggcgtgcgg ttcgaagagg tgtcggtcgg ctatccgcga gaatggcggc
     2341 gccgcaagcc atatcgatgg ctgcggcaga tactgcctga tccgaactac gcgccgggct
     2401 acctcaatga gttcggcggt cacttccaga tcacgcagga gatgcgggag cacgccgaga
     2461 aggtacagga gcgcatcagg gccaggcagt catgacgacc gcaccgttgc tcggcggtgt
     2521 gctacccggt gtcgcagcct tggcggccgc cgaaacatac gacgacccac cgggattggc
     2581 cccgctgccg gaagaggaac cattgatcgc caagtcggtg gccaagcggc gcaacgaatt
     2641 catcactgtg cggtactgcg cgcggcttgc cctggaggag ctggggatgc cgccggtccc
     2701 gatcctgaag gggaacaagg gtgaaccgtg ctggcccgac ggcatcgttg gcagcctcac
     2761 gcattgcacg ggcttccgcg gcgcggccgt cggcaagctc ggcacggtgc gctcgatcgg
     2821 cctcgacgcc gaaccgcacg acgtgctgcc caagggtgtg ctcgacgcga tcagcctgcc
     2881 ggccgaacgt actgaattac aggcgatgcc cgacggtttg cactgggatc gaatcctgtt
     2941 ctgcgccaag gaggcgacgt acaaagcgtg gtttccgctg acgcaccgct ggctcggctt
     3001 cgaggatgcc cacatcacgt tcgacgtcga cgacagcggc acaacgggtg gcttcgtatc
     3061 gcgcattctc atcgatccgg ccgccgaatc tggcccgccg ctgcagacgc tgtcgggacg
     3121 ctggtcggtt cgggacggtc tcgcgctgac ggcgatcgtg ctgtgaccga cgccggtttg
     3181 gtcgtcgtcg acaagcccgc cggcgtcacc agccatgacg tcgtgggccg gtgccggcgc
     3241 atcttcggca cccgcaaggt cgggcatgca ggcacactcg acccgatggc caccggcgtg
     3301 ctcgtcgtcg gaatcgaacg ggccaccaag atcctcggcc tgttgactgc caccgacaag
     3361 tcgtatgccg cgaccattcg cctgggccag acgacatcta ccgacgacgc cgaaggcgaa
     3421 ctcctgcagt ctgtttcggc cgacgcagtc accgacaccc aggtcgacga ggcggtcgct
     3481 gcgctgcgcg gggagatcgc ccaggtgccc tcggcggtca gcgccatcaa ggtcggcggc
     3541 aagcgcgcct acaagctggc ccgcgagggg cagaccgtcg aattggcgcc tcgcatggtc
     3601 cgcatcgacc gattcgatgt gcggtccatc cggcaccacg acgggctcat cgacctgaac
     3661 gtcgaagttg actgctcgag cggcacgtac attcgggcgc tggcccgcga cattggcgct
     3721 gccctcggcg tcggcggcca tctcaccgcg ttgcggcgca cccgcgtcgg gcggttcgga
     3781 ctcgaccaag cccgcatcct cgacgacctc gccgagtcac cgcgcctgag ttacaccctc
     3841 gacgaggcgt gcctgcaatc gtttccgcgc cgcgatctga ctgccgacga aacggaagac
     3901 acccggcacg gcagaccgct gaaacccgct ggcttcgatg gggtttacgc agcgaccgcc
     3961 ccggacggtc aggtgatcgc actgctgacg gacggaccga accggacgac gtcggtggtc
     4021 gtgttgaggc ccgctacgct gtaatcgctt taccgcgaat gcgacagcgt ggcagcctat
     4081 cgcgcgcata ctgcccaaat gggatcgccc tacaagcttc tgcagctcgc actcgccgta
     4141 ttcggcgcaa tcatgatcct gctgtatcca ctggccctgg tgtggccgtc ggggtgggca
     4201 tggcatcacg gcgcacccca ccattcggtg tacttcatga tgatcgtcgg catctacgcg
     4261 acgctgggag ccttcctgtt gaacgccgca cgcaaccccg cggccaatct cagcctgatc
     4321 tggttcgcgg tgtggtccag cgtcgtgcat gccgcgatca tggcggtgca atccttcacc
     4381 gagcaccaga tgggacatct ttggggagat gtcgccgcgc tgctcttggt cgcaatcgtg
     4441 ctatcggcgc tcgtgctgtc ggctgggctg aagcagccgg cacccgatag agccggaacc
     4501 gtggcgcgtt agccgaccac ccaaatcgct tctgcggcag ggcttcccag gtcgaccgtg
     4561 gtgtcatcgg agccctcggc tccctcgatc gccaccgaga tcatcccggc gaagtcacgc
     4621 cgcgccagca cccgcagccg agaatccagg ctgatgccga cactgtcgaa ataccgcagc
     4681 atctccgggt cggcgtcgga gattcgcgcc accgtgcccg cgtcaccgtt ctgacacacc
     4741 gacagctgcc gcgcatcggg cgtcggcacc cgtccatcgg ccgccgggat cggatcgccg
     4801 tgtgggtcgc gggtcggatg acccagcttg gcgtcgatgc ggtcgagcat ccgatcggac
     4861 accgcgtgct cgagcacctc ggcctcgtca tgcacctcgt cccagctgta gcccagctcg
     4921 cgaacgagga acgtctccat cagccggtgc cggcgcacca tcgccaacgc cgcgcggcga
     4981 cccgcctcgg tcagcgtcac ggcgccgtac ttctcgtgat cgaccagccc ctggtcggcg
     5041 agcttgcgga tggactccga cgccgtgctg gccgacacgc cgatccgctc ggcgagcagc
     5101 ttggtgctga ccttctcccg cgaccactcc tgggcggtcc agatgacttt gaggtagtcc
     5161 tgggcaaccg tggttaggtc gcgagggttg ccgtcgggac tcacgcttag aaagtttagg
     5221 caatcttcac ctgctctggg ctactagcga agccgtacga aaccgcgcgt cgtaggcttg
     5281 ccgctgtgca gcgctggcgg gggcaagaag acatcccgac ggactggggc cgatgcgtcc
     5341 taacgatcgg cgtcttcgac ggtgtgcatc gagggcacca ggagctgatt ggccacgcgg
     5401 tgaaggccgc ccgggctcgc ggcgtgccga ctgtgctgat gacgttcgac ccgcacccga
     5461 tggaggtcgt gtttccaggc agccacccgg cgcagttgac caccctgacc cgtcgcgccg
     5521 agctcgtcga ggaattgggc atcgacgtgt tcctggtgat gccgttcacg accgatttca
     5581 tgaagctcac tccggatcgc tacatccacg agctgctcgt cgaacacctg cacgtcgtcg
     5641 aggtggtggt gggggagaac ttcaccttcg gcaagaaggc cgcgggcaac gtcgacacat
     5701 tgcgcaaagc cggcgaacga ttcgggtttg cggtgcaagg catgtcgctg gtgagtgagc
     5761 accaccagaa cgaggacatc acgttctcgt cgacctacat ccggtcctgc gtggacgccg
     5821 gcgatgtcgt ggcggccacc gaagcgcttg gccgtccgca tcgcgtcgag ggcgtcgtcg
     5881 tccgcggcga gggacgcggc atggtgctgg gcttcccgac cgcgaacgtg gcgccgccga
     5941 tgtactcggc gatccccgcc gatggcgtct acgccgcctg gttcaccgtc ctcggacacg
     6001 gcccgatcac cggcaccgtc gtccccggtg agcgctacca ggccgcggtg tcggtgggca
     6061 ccaaccccac cttctcgggg cgcacccgca ccgtggaggc cttcgtactg gacaccagcg
     6121 cggacctgta cggccagcat gtcgccgtgg acttcgtcgc ccgcatccgc ggccaggaga
     6181 agttcgagcg gatcgaggag ctggtggcga cgatgggcga cgacaccgag cacgcccgca
     6241 cgattctgtc cgcgggctga ccatgcgata aactgcctgg cgaccacgac gtgtgctgca
     6301 gttcgcggcg gccacgtcgc atttttctga tcgcggaccg atggatatgg agttatttcg
     6361 tggcgctcac tgccgaacag aagaaggaca ttctgggcca gtacggcctg catgacaccg
     6421 acaccgggtc gccggaagcc caggtcgcgc tgctgaccaa gcgcattgcc gatctgaccg
     6481 agcacctgaa ggtgcacaag cacgaccacc actcgcggcg cggactgttg ctgctggtcg
     6541 gtcggcggcg ccggctgctc aagtacgtcg cccaggtcga cgtggagcgc taccgctccc
     6601 tgatcgatcg gctcggcctg cgccgctgag cggcgcatct ggcccacgcg ctgactcgac
     6661 cgagaattcg gccccgtacc tgctgcaagg tacgatggct tcgttcgcca gccgtttcgg
     6721 ctcgaacgat tgggtgcggt tttcgcagat ccgcgagcgc cgttcccgcg tctcatatcg
     6781 ggacccgcct gatcgcgcgg tcttcggtag tggctgccgg gtctgatacc cgacagcttc
     6841 gatcgatggc cgtagccgca tcgaggccgg tggttcaggc gctgtgacga cgcgaaacag
     6901 ctgaacactg aatccagaga ggccatacgg acgcccatgt ctgtagttga aattgaagac
     6961 ggcgtgtacg aatcgaccgc cgtgatcgac aacgggagct tcggcacccg caccatccgc
     7021 ttcgagaccg gacggctggc ccagcaggcc gccggctcgg tagtcgccta ccttgacgac
     7081 gaaaccatgc tgctgtcggc gaccaccgcc agcaagaacc cgaaggatca cttcgacttc
     7141 ttcccgctga cgatcgacgt cgaagagcgc atgtacgccg cgggccgcat ccccggctcg
     7201 ttcttccgcc gtgagggccg gccgtccacc gacgcgatcc tgacctgccg gctgaccgac
     7261 cggccgctgc gcccgtcgtt cgtcagcggc ctgcgcaacg agatccaggt cgtcgtcacc
     7321 gtgatgagcc tggatcccaa cgatctgtac gacgtggtgg ccatcaacgc ggcatcggcg
     7381 tccactcagc tggcgggtct gcccttctcc ggacccgtcg gcgcggtgcg cgtcgcactg
     7441 atcgacggca cctgggtcgc gttcccgacc gttgagcagc ttgagcgcgc cgtgttcgac
     7501 atggtcgtgg caggccgtgc gctcgaagac aacgacgtgg ccatcatgat ggtcgaggcc
     7561 gaggccaccg agaacgtcat cgagctcata gcgggcggcg cgcaggcgcc tacggaagcc
     7621 gttgtcgccg aaggccttga ggccgccaag ccgttcatcg ctgcgctgtg cacggcgcag
     7681 caggagctgg ccggtcgtgc ggccaagccg accggcgagt accccgtctt ccccgattac
     7741 ggcgacgatg tctacaactc ggtggcctcg gttgccaccg atgaattgtc gaaggcactg
     7801 accatcgccg gcaagcacga acgcgacgac cgcaccgacg agatcaaggc cgaggtgctc
     7861 gagcgactgg ctgagcagtt cgccgggcgc gaaaaggaga tcggtgctgc gtaccggtcg
     7921 ctgaccaaaa agcttgtgcg ccagcgcatt ctgaccgatc acttccgcat cgacggtcgt
     7981 ggcatcaccg acatccgcgc gctgagcgcc gaggtcgctg tggtgccaag ggcgcacggc
     8041 agtgcgctgt tcgagcgcgg tgagacccag atcctcggtg tcaccacgct ggacatggtc
     8101 aagatggccc agcagatcga ctcgctggga cccgagacca ccaagcgcta catgcaccac
     8161 tacaacttcc cgccgtattc gaccggtgag accggtcgcg tcggctcgcc gaagcgacgc
     8221 gagatcggcc acggcgcgct ggcggagcgg gcgctcgtcc cggtgctgcc gagcatcgag
     8281 gagttcccgt acgcgatccg ccaggtgtcg gaggcgctcg gctccaacgg ctcgacgtcg
     8341 atgggctcgg tgtgtgcgtc gactttgtcg ctgcttaacg ccggtgtgcc gctgaaggct
     8401 ccggtcgcgg gtatcgcgat gggcctggtg tccgacgatg tcgaggtcga gggaggcggc
     8461 accgagcggc gcttcgtcac tctgaccgac atcctcggag ccgaggacgc cttcggtgac
     8521 atggacttca agtgcgcagg caccaaggac ttcgtcaccg cgctgcagct ggacaccaag
     8581 ctcgacggca tcccgtcgca ggtgcttgcg ggcgcgctgg cgcaggccaa ggatgcccgg
     8641 ctgaccatcc tcgaggtgat ggccgaggcc atcgacagcc ccgatgagat gagcccgtac
     8701 gcgcctcgga tcaccacgat caaggtgccg gtcgacaaga tcggcgaggt catcgggccg
     8761 aagggcaaga tgatcaactc gatcaccgag gagaccggcg cgcagatctc gatcgaggac
     8821 gacgggaccg tgttcgtcgg cgccagcaac ggcgaagccg cgcaggccgc gatcgacaag
     8881 atcaacgcga tcgccaaccc gcagctgccc aagatcggcg agcggttcct cggaaccgtg
     8941 gtcaagacaa ccgatttcgg cgcgttcgtg tcgctgctgc cgggacgtga cgggctggtg
     9001 cacatctcca agctcggcag gggcaagcgc atcgccaagg tcgaagacgt ggtcaaggtc
     9061 ggcgacaagc tgcgcgtgga gatcgccgat atcgacaacc gcggcaagat ctcgctggtc
     9121 ctcgtcgagg aggaagagtc cgctcccgcc gaggcgccgg cagc
//
LOCUS       DASRJA010000147        29976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2134769,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000147 DASRJA010000000
VERSION     DASRJA010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 29976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29976
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134769"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VFC01_06840"
     CDS             complement(<1..125)
                     /locus_tag="VFC01_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN79391.1"
                     /translation="MFPAVKAQAQEAESAGFDTVLVMDHFYQLPTLGKPDEPMLEA"
     gene            complement(476..1684)
                     /locus_tag="VFC01_06845"
     CDS             complement(476..1684)
                     /locus_tag="VFC01_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN79392.1"
                     /translation="MSSSRDSDGAPVDPPIPVPDVPGADATAGGLPRRVDLTPRQRLI
                     VDSSAMADIALRTAIASLLSVAMVPTMLGTALRRSASRAERDYLRFYAELASARDPEL
                     SFPAPTSEPRISSRPANPIAEWMAKGSVRNIRFDSSFEAVNPALREQCRGYVRNNVVH
                     AQHWVHDDGPHPTLCVIHGFMGSPYLFNGLFFSLPWFYRSGYDVLLYTLPFHGARAEK
                     GSPFSGYGYFAHGFAGFAEAMAQAVHDFRSLIDYLEFTGVDRVALTGMSLGGYTSALI
                     ACVEDRIQAVIPNVPVVTPDRTVDEWFPANYVVRLRDLIAGAEDELVHEATKYPSPLN
                     YPPLVPKDRRLIITGLGDRLAPPEQAELLWEHWDRCAFHWFPGNHVLHVSQPDYLRRM
                     TRFMRDFMFK"
     gene            complement(1701..3560)
                     /locus_tag="VFC01_06850"
     CDS             complement(1701..3560)
                     /locus_tag="VFC01_06850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN79393.1"
                     /translation="MTALVDQPVSVETIAPARTGHWSRWTLGTLLAATAALYLWNLSA
                     SGYGNTFYAAAAQAGSQSWSAWFFGSLDAQNFITVDKPPASLWVTGLSVRLFGMNSWS
                     VMAPQALMGVAAVAVLYCAVRRAVPNPNHGAAAGLLAGAVLAVTPAAALMFRFNNPDA
                     LLVLLLTVAAYCLMRAAMAASWRWLVLAGVVMGTAFLTKMLQGFLVLPGFGLAYLCVA
                     PTSWGKRLAHLLGAVAALIAAAGWWVLAVQLTPASARPYIGGSKDNTVLDLALGYNGI
                     DRILGRHHEGKPLGDWGSSSLPMMGGRTDLHRLFTGEMANEISWLIPVAFFVVAFGMY
                     LAARRALSRSELCALLMWGAWLTVTGVVFSFMGGMVHPYYTVALAPAIAALVGIGAVW
                     AWQRRSNWDGRCVLAAMIVLAAVPSSVLLHRHAFGHSWLPWLIVVLAVVSGSAVLWPR
                     PPIALLVSGCLTGLAGTTVFSVATAATPHHGTIPTAVRSSQVTGAWGNDEATNTQLAG
                     VLAATHTQWSAATNGSQSAASLEIASGTSVMAVGGWSGDPVPTLQQFIDDVHAGKISY
                     YVEAGRGPNSPGVHGEVIRSELHTASHTREIADWVTAHYPGTFIGGSTVYRLI"
     gene            3651..4412
                     /locus_tag="VFC01_06855"
     CDS             3651..4412
                     /locus_tag="VFC01_06855"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HZN79394.1"
                     /translation="MWNPDVYLTFADHRGRPFFDLLSRVGAEKPRWVVDLGCGPGNLT
                     ATLAQRWPDTVIEAWDSSPEMVEAARERGVDAHIGDVRAWTPRPATDVVVSNATLQWV
                     PEHAELLVRWADQLESGSWIAMQVPGNFDAPSHRAVRELARRDQWSEPLRDFPFREGQ
                     VDDPSGYAGLLSDAGCTVDAWETTYIHELTGDNPVLEWITGTALTPVKSRLNEEQWEQ
                     FRQELIPMLHAAYPGRPDGTTFFPFRRIFVVAQVR"
     gene            complement(4400..6286)
                     /gene="cysC"
                     /locus_tag="VFC01_06860"
     CDS             complement(4400..6286)
                     /gene="cysC"
                     /locus_tag="VFC01_06860"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HZN79395.1"
                     /translation="MTAPPKTITRQLLRITTAGSVDDGKSTLIGRLLHDTDSLPLDHL
                     EAVTDEEGVADLAALSDGLRAEREQGITIDVAYRFFSTDTRSYILADTPGHERYTRNM
                     FTGASNAHVAVLLVDARAGVLRQTNRHARIAKLLGITHFVATVNKIDLVDFDHARFTE
                     VENELRLAAARLGGVDITVIPVAAKHGDNVVHRSDRTPWYQGPTLLEYLESVELTAPQ
                     PEPSKLRLPVQWVSRPTADQRRRYTGRLSGGTLQVGDPVISLPAGTRSSVTVVDALDD
                     ERTTAVAPLSVSIELADDIDVGRGDVFVSAADDATHPVLARELDATVCWFIDSPLRAG
                     DRLALKQGTRTVRTTVQALHSRLDPETLDELDNPVELVLNDIGEVTLRTSSIVVADAY
                     TDNRDSGAFILIDETTNDTVGAGTILEPREVQPGVQTRNDIRWHPSSLDREYRWTATG
                     QHGATIWFTGLPASGKSTIAVAVERALVESGQVAYLLDGDNIRHGLSDDLGFSAGDRA
                     ENIRRVGHLTRLFADAGVVALASLVSPLKSDREIARALNNAARLPFIEVYVCTPRAEC
                     ERRDPKGLYARARAGELKGLTGVDAPYEAPEDPDLVLDTTGADINQLVAQVIDLLNER
                     RQRT"
     gene            complement(6286..7203)
                     /gene="cysD"
                     /locus_tag="VFC01_06865"
     CDS             complement(6286..7203)
                     /gene="cysD"
                     /locus_tag="VFC01_06865"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HZN79396.1"
                     /translation="MTTAFDTVHVDELRLLEAEAVHIIREVVAELERPVLLFSAGKDS
                     IVLLRLAEKAFRPNPLPFPVMHVDTGHNFPEVIEFRDRRVTGQGHKLIVASVQESIDI
                     GRVPDPGPGASRNRQQTRTLLDALEAGEFDAAFGGARRDEERARAKERILSFRDEFGQ
                     WDPRAQRPEPWSLYNGRIKKGEQVRVFPLSNWTELDVWRYIELENLELPSIYFAHERE
                     VFERDGILLAVSEYAKPVNGEIAAVEWVRYRTVGDLTITGAVRSEATDIDRVIAEISA
                     ATVSERGETRADDRTSAAAMEDRKREGYF"
     gene            complement(7200..8000)
                     /locus_tag="VFC01_06870"
     CDS             complement(7200..8000)
                     /locus_tag="VFC01_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HZN79397.1"
                     /translation="MSHSTAYLVLASQRSGSTLLVESLRATGVAGEPGEFFQYLPTTS
                     QSPQPRQWFEGVEDQSILRLLDPLDEGKPDLAPPEIWRDYIRTVGRTPNGIWGGKLMW
                     NQTPLLLQRAAGLPGRSGDGLLSAIRDVIGSDPVLIHVYRPDMVSQAVSFWRAVQTRV
                     WRGRPDPARDSRAEYHAGAIAHVVSMMRAQEEGWRKWFAAEGVEPMEVAYPVLWRNLT
                     QIVGTVLEALGQDPGLAPAPVLERQADQRSDEWVDRYRADAEREGLPT"
     gene            complement(8067..9437)
                     /locus_tag="VFC01_06875"
     CDS             complement(8067..9437)
                     /locus_tag="VFC01_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZN79398.1"
                     /translation="MTERRDNVLIVHWHDLGRYLGAYGRADVSSPRLDRLATEGILFT
                     RAHATAPLCSPSRGSLFTGRYPQNNGLLGLAHHGWEYRAGVRTLPHILIESGWYTALF
                     GMQHETSYPSNLGFEEFDVSNSYCEYVVEHATRWLTDPPEQSFLLTAGFFETHRPYPR
                     DRYEPADADDVAVPDYLPDNAEIRQDLAEFYGSISVADAAVGQLLDTLDATGLDESTW
                     VVFMTDHGPALPRAKSTLYDAGTGIALIVRPPRRTRIEPQVYDDLFSGVDLVPTLLEL
                     LGIDIPTDIDGLSHARNLQISPQEAVRTEVYTTKTYHDSFDPIRAIRTKEYSYIENYA
                     ARPLLDLPWDIAESAPGRIMEPLVLAQRPERELYDLVEDPTESHNLLGPDVTDKAEAI
                     ANDLALLLDDWRHKTNDVIPSDFAGTRIAERYTETYLRIHGPQVTSRSAIASERGIED
                     DHSSGQ"
     gene            9608..11260
                     /locus_tag="VFC01_06880"
     CDS             9608..11260
                     /locus_tag="VFC01_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HZN79399.1"
                     /translation="MFDGSLPEVRELAALEDAALVDAAAGWARTENAACARKTAVMAE
                     LFARRTGLPAGERELWWVDPEASVGAELAASQNISTWMALAQAHRGVVLADRLPKVAA
                     LFAAGLISEVLVRTIEYRTDLVTDDEAIARVDVLVAEHVTAWGPLSAKKLEQAIDAIV
                     DQVDPGALRRSRKTACQRDVQFGSPSDEAGFTSMWARLYAPDAAVLQQRVDEMARGVC
                     QDDPRTLGERRSDVLTALATGITQLACGCGSTDCPAAAPDATPATPAVIHMVAHADTV
                     DAAQTQNTTPPPDANGPDADPAPAPGPDLDKLASSEPVDDDPAPAADAAGVPPAFCPA
                     PVAESASAPPAFCPAPPAFVIGGGVLPAPLLAAALERATVREIRHPGDAPAERHYRPS
                     RELADFVRCRDLTCRWPGCDKPAYGCDIDHTVPYPIGPTHASNTKCYCRFHHLLKTFL
                     CGGDGWREQQLPDGTLIVTSPTGHTYTTKPGSAVLFPALCRPTGTLWAPGEEPKVWPR
                     GDRGLMMPKRRRTRAQNLAKRIEAERKLNDDYIAERNKPPPF"
     gene            complement(11274..11756)
                     /locus_tag="VFC01_06885"
     CDS             complement(11274..11756)
                     /locus_tag="VFC01_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79400.1"
                     /translation="MAVFLRKLLGIGKLPDEIRTEVEAEGIIHIAEYVPVTRRFSGRV
                     PGKRSAGSIASYVGSLVLTNQRVLATLSSVPKLAGRTIDLRWDAPQTGAVKAQLSETG
                     LVLDANVADVDPRCTGQLSLHYKDALPEDVLMRLPRRSLAFDVPPEYVFRAVGVPYHP
                     "
     gene            11819..12490
                     /locus_tag="VFC01_06890"
     CDS             11819..12490
                     /locus_tag="VFC01_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZN79401.1"
                     /translation="MTPRKTWLIISAAAAVAVYALMWIGYASHWNWLTTIDSLWLEAT
                     HRYGAPRPGWVTAWNVFCTVLGPTAFRLATVAVIISSLVRRNVRVAMFLVISVELAGL
                     VTEIAKYAADRPRPITALASAPSTSFPSGHALGVMVGVLALLTVVLPVVRQPLRALLV
                     ACGAAVVVLIGVGRVVLNVHYPSDVLAGWALGYAYFVACLLMVPPSRPVTVTDEIPAA
                     LGTSR"
     gene            complement(12444..13937)
                     /locus_tag="VFC01_06895"
     CDS             complement(12444..13937)
                     /locus_tag="VFC01_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZN79402.1"
                     /translation="MELLPLRGRLRGIRQIGEGLGTLDREVFEAIAESPSPLLDATMP
                     RLTRAADHSKVWLALAAVIAVLGGPSARRSATRGVLSLAATSLFTNLVAKRGWRRPRP
                     NRLSVPIARRGHRSPTSNSLPSGHSASAAAFAVGVGLEKPPLGLGLALLAGLVGLSRV
                     ATGVHYPGDVFAGFGIGAGIAVLGGRVVPPVVTAKIPVADPLRVDTPERPDGAGVVLV
                     VNPASGGGTGARVADEVRESLPQTEIVELGEDDDVEEVLRKAVERAEVLAVGGGDGTV
                     SCAAGVAAEAGVPLAVFPGGTFNHFAKDIGCDSVDKTVEAIQRGSVACVDMVCLNEGR
                     MVINTASIGAYPTFVRTREKLEHKVGKPLAGLYAMLRTLEQDRPVRIVYDNKTLQTSL
                     FFLGNSTYLPSGFAPSRRTRMDDGLLDVRILETGRRFSRVRIVAALALGRLERSPLYH
                     ELRVPEFTFKTVDGPTVLAHDGEVGTEVTEATFTSKYRALPVFRPLP"
     gene            14024..14677
                     /locus_tag="VFC01_06900"
     CDS             14024..14677
                     /locus_tag="VFC01_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZN79403.1"
                     /translation="MRRLLALLCAGAVSLTAAPVAHAEFTPWFAPQVGNATQVISVVG
                     SGGSLAKIDVFQRGAAGWEAVRAGIPAHVGANGFAAETHDGNMATPLGIYTLDFAFGT
                     APNPGGGLQYVQVGPDHWWDGDMKSPTYNTMQVCKKSQCPFDTSPDSGTENLEIPQYA
                     HAVVMGVNKARVPGNGGAFFLHTTDGGPTAGCVAIDDGTLVEIMRWLRPGALIAVTQ"
     gene            complement(14681..15139)
                     /locus_tag="VFC01_06905"
     CDS             complement(14681..15139)
                     /locus_tag="VFC01_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN79404.1"
                     /translation="MGDIDEIQQVKYRYLRALDTKHWDDFADTLTEDIIGDYDASIGE
                     SHHFTDRDTLVEFMRTSMPANIITEHRVTHPEITVNGNEASGTWYLQDRVIVPDFNFM
                     LIGAAFYHDQYRKTGDGWKISKTGYDRTYDATMSTENLNFKLKTGRALNI"
     gene            15186..15947
                     /locus_tag="VFC01_06910"
     CDS             15186..15947
                     /locus_tag="VFC01_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016693925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79405.1"
                     /translation="MKFIDSYVRSPFAGIAPWVLMGVVSGPGRFEEAASAALGLALLT
                     LWVGSRRGVPVHLLEVFTVGYFGVLAVLGLVASDATIDWLELWAGELSNIALAAFAIV
                     TLLIRKPFTLAYAKDTTPREHWDDPLFLRINYVISAVWAGAFLVSAVVGAYGDAVLRD
                     NDNFWTAWIVPLAAVIFAGAFTEFYPDRATGEATSWARILDWLPPFVLVTGIVGWVSD
                     AVPDGVGIGLIVVGIVGSAAMRKLFPAQAEATEPL"
     gene            complement(15931..17436)
                     /locus_tag="VFC01_06915"
     CDS             complement(15931..17436)
                     /locus_tag="VFC01_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN79406.1"
                     /translation="MTSLWLADRIDRSIPPNPLLEADLSADVVVVGAGLTGLTTAVLL
                     ARAGKDVLVLEAHSAGAGATGNTTAKISLLQGTKMSKIVGKHGPKTAAQYVQGNREGQ
                     EWLVQHCEAHGISVQHEAAYTYAQSEDGVAAARAELEACTAAGLEVEWADDADVPFPY
                     HGGVRLAHQAQFDPMPLLDSLIVELEERGGRIAQSVRVQSVSGKDDALALHVRTTDGD
                     EFDVHAKQCVLATGIPILDRGGFFARLKPSRSYCMAYKVPGNITHGMYLSADSPTRSV
                     RYAPTPGGDRLVVGGAGHPVGREKSPACSVQELDSWAKKNYPGAMQTHYWSAQDYTPA
                     DELPYVGPILPGNEKIFVATGFDKWGMTNGTAAALALSSRILGGRMDWANAFASWSPH
                     ELSGIPKALQANLEVGFNLAKGWITPVLRIAHRTPESGGVVSGPPWDLEARCVVNGVE
                     HQVSPVCPHLGGIVNWNDADESWDCPLHGSRFAPDGTLLEGPATRNLTAAQ"
     gene            complement(17633..17825)
                     /locus_tag="VFC01_06920"
                     /pseudo
     CDS             complement(17633..17825)
                     /locus_tag="VFC01_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016706137.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17993..19780
                     /locus_tag="VFC01_06925"
     CDS             17993..19780
                     /locus_tag="VFC01_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZN79407.1"
                     /translation="MSDPLGLSIGTTNLVAARVGNQPVLRRSVLTLFGHAAPEVGVPS
                     AHSDGIVLGGFVERVGDPVPLVAADGSPHQADQLLVDALQAMVYASGGQPSPETAIAV
                     PAHWSTSTLWALRNAMRASPTLAPNGMPVRLVSDAVASLTALNANPGLVSQGVVALLD
                     FGGGGTNITLADAASRYEPIEDITRYAEFSGDLIDQALLSHVLEGIANSGSVDPAGTA
                     AVGSLARLREECRNAKERLSAETVTELIAELPGYRSGIRVTRTELESLIAPPLTGVLT
                     ALENALERNRISWSNVSAVVTIGGGASIPLITARLSEHAQAPVLTTPQPALDAAVGAA
                     LFAAYGAQADAKTGVAPALPVDVPDPVDAPGSATFRALAWSQDDDTEDVVPYTGDVYD
                     TGVTAARPQVQYMPATGPIEEPRSTWNRVPQLVFGLAAVVAVVAVGGVAIALTSATDS
                     TKATEPTKPATSAPAPTSAAPPPSTPPPVETVTVTSEPPPPPPPTTTVEPVTTTHVTT
                     TTQPTTTTRTTTTTTTTTTTTTTTTTTTPTTTTTTTTTPPMTTSYITVPFVPVPIPIQ
                     VPNQGPQSQAPQYEAPQYPYQYQQPQQLP"
     gene            complement(19765..20781)
                     /locus_tag="VFC01_06930"
     CDS             complement(19765..20781)
                     /locus_tag="VFC01_06930"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HZN79408.1"
                     /translation="MSKGDPLLEVTDLVKHFPIKSGVVVDREVARVRAVDGVSLSLRE
                     GETLGLVGESGCGKSTLCRAILQLTQPTSGSVKFLGEELVGRSRRDLRPLRRDMQMIF
                     QDPFASLNPRKRVGQIVGDPLELHGLAKGAELKRRVQDLLDRVGLSPEHYNRYPHEFS
                     GGQRQRIGIARALALQPKMIVADEPVSALDVSVQAQIINLLKELQDEFGLSYLFVAHD
                     LGVVRHVSDRVAVMYLGKVVENSETEDLYRRPIHPYTNALLSAVPIPDPKRNAARERL
                     VLQGDVPSPIDPPPGCSFHTRCPWATEICTTDEPALLEHAAAHVAACHHPRNVEEPVA
                     QGSC"
     gene            complement(20778..21779)
                     /locus_tag="VFC01_06935"
     CDS             complement(20778..21779)
                     /locus_tag="VFC01_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN79409.1"
                     /translation="MSTILKVDDLNVSFATEDGVVQAVGGVSFELRAGEVLAIVGESG
                     SGKSVTAQTITGLTRASNARITGTVTYRGRELNGLEDDELRDVRGEQIAMVFQDPMSS
                     LNPVYRVGDQITEMIRAHRDLSKREAADRAVELLRSVGIPNPERRVRHYPHEFSGGMR
                     QRVMIAMALALEPDVLIADEPTTALDVTVQAQILRLLDQLNRDRDLAIILITHDLGVV
                     AEIADRVLVMYAGQIVENATLDETFYDPQHPYTWGLLGSLMRLDQTRGARLAQIPGQP
                     PSLLAPPKGCRFAPRCPHEFDKCSELPPLEPRVGGNHLDRCWLDPEEKKSRRAQVIS"
     gene            complement(21776..22738)
                     /locus_tag="VFC01_06940"
     CDS             complement(21776..22738)
                     /locus_tag="VFC01_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN79410.1"
                     /translation="MARFIVRRLLGMIAVLFAISVIVFLIFNVIPNSDPAARIAGKNA
                     DPALIARVSADLGLDQPLPVQYLTMMKQIFTGQLMSYAKDRNVVQQIWDGVPATFSLC
                     IGAAVIWMALAVLFGYMSAIHAGKFTDRALTILSLVGISMPVFWLAAILLYFLTFKIQ
                     LFPTGSYVPLTENPVGWASHLILPWFTLAVLFVGFYSRMLRSNMLDAMNEDYVRTARA
                     KGLSERQVRIRHVLRNSMIPIVTLFGLDFGQVVGGGAILTETVYNLNGVGLYAGEAIR
                     SLDLPPLIGVTLFGAFFIVLFNTIVDFAYAVLDPRIRLGEAAPA"
     gene            complement(22738..23775)
                     /locus_tag="VFC01_06945"
     CDS             complement(22738..23775)
                     /locus_tag="VFC01_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN79411.1"
                     /translation="MVVPVGELEVPGEPPLPTGVPASQIQGRNPWYLAWLRLRRNKVA
                     LVCGAVFVLIVLFCLAAPLWAEHVAHTGPNENHITEKVVIDGQPTNVVAPDGTPIGPG
                     LHGQYLLGADQNGRDVMVRLMYGGRTSIYIGLAAAAVTTVLSVIVALLAGYYRGWIDS
                     VLSRIMDVIWAFPVLLLGIALGTALAIGGLKIGPLAIAGDSIWIPIMIIGFVYVPYMA
                     RPIRGEILALREKEFIEAAVAQGKGPLRIMFSELLPNIISTIIVFFTLNIANNMLLES
                     ALSFLGAGVRPPNASWGTMIADGYEMIYTAPHLTIVPGLMIIATVLSLNVFGDGLRDA
                     LDPRAKIRLEH"
     gene            complement(23782..25434)
                     /locus_tag="VFC01_06950"
     CDS             complement(23782..25434)
                     /locus_tag="VFC01_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN79412.1"
                     /translation="MNTLRKVVAIVCITPLAAFGVAACGSDKGGGGGGGGGGGDINVA
                     LTSFPDYIDPQLSYTLEGWEVLWNVYTPLLTYKHAKGKEGTQVVPALAEKMPDISPDG
                     KTYKLKLRQNMKYSDGTPIKASDFAYGIQRLFKTDSGGSVFFNGIVGAKDYADGKANT
                     ISGIKTDDNTGDITIELAEANGTFENVLGLMFAAPVPPSTPLDKDATNNPPPSSGPFM
                     ISKVEAPRTLTMERNPNFKTVKDAGADEVVDASVDKITVTENKNNSAQVTDIEQNKVD
                     FMVDPPASDRLQEVKTRYGNRFRMEDSINTYFFWMNTQKPPFNDLKVRQAINYAVDPE
                     ALNRIFGGRMHPTQQVLPPGMPGYSEYKLFPGPDMNKAKQLIAEANPADRNITVWTND
                     EPDRKRIGEYYHDLLTQLGFNATLKVIAGDVYFTTIGNESTPDLDTGFGDWFQDFPHP
                     DDFFRPLLHGDSILPTNSNNWSRVNIPANNAKQDELLTKLIGDGGVEQQYADLDKAYM
                     EQAVWAPYGNERFTTFLSERMDFDKSYHHLLFNQDYSSFAIK"
     gene            25737..26006
                     /locus_tag="VFC01_06955"
     CDS             25737..26006
                     /locus_tag="VFC01_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79413.1"
                     /translation="MLSSLLARRLAAVAGGVAIAAMVGVTASCAKEEKKAPETTTTTT
                     TTTTTSASPAPPPSPTEKSINPTGGNLFTPPVYAPPAPTAIPGDH"
     gene            26041..27618
                     /locus_tag="VFC01_06960"
     CDS             26041..27618
                     /locus_tag="VFC01_06960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HZN79414.1"
                     /translation="MSAVLAAGVLVLATGVRAASVSDDRPVIGGPLALLLASSTDLGP
                     SRDGGAQLTVTLADSARPATLIAWASAHDMWVRWRPGDTWAVVEGAAQDVATAFAVPV
                     NDYRGRKGQVFYASPQQPSIPETLRASVTGIGRILGYTPHREARPGFLPLDVPSQGLT
                     PKALLEAYNANPLAAAGFTGKGATIVFFSFSGYDQKDLDAFADLSGLPRFTPVLVGGQ
                     PGPSGAETAMDLEVAHAIAPDAQKVVVNARPTVEGDGAYEKIGQLFESVDRQFPGAVW
                     SLSIGWGCDALISAADLAPIEAALAAAQKRGTSAFDASGDTAGLECKGGDEWSSNPGP
                     DDVGLDAISSLPAMTSVGGTTLSTDDQGRWLAERAWVDSPLSQGTSGGVSVLYDRPAW
                     QERLSVERDVDRRRLAPDVAAVADPFTGVRIVFDQQELVGAGTSQAAPIWAGLAVLMN
                     QYLLAHGGRPLGHINPSLYRVAAGANLPAFRDVGLGGNAVDISQPGYDLVTGLGSPNV
                     DYLVRDLLDIQRGVAPR"
     gene            27615..29492
                     /locus_tag="VFC01_06965"
     CDS             27615..29492
                     /locus_tag="VFC01_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZN79415.1"
                     /translation="MTTGVPVMRCPVCNADVPAAAFCGACGAHLSPRRGDGRGMLRPV
                     AYGAAPGEHVLRLSVASTLFPHLPHRSRAPFRVALAVLFLTLVVLAFLGWQAPLIAVG
                     ALGFPMVFVLYLHETDIDDDLPLSSLVLTAVLAIGLGIGWALLTGVIVAHSYDVTLSD
                     ASSEEHSIPVEGIAIPAGTAVLLVLPAVVMRLLRPGTRESLDGFVIGALSAIAFNAAA
                     TLTRLSPQFATGPTAHDRPASSLLAEAGIQGVAMPLTAAALGGLAGVALWFARPGDKD
                     RRPAIVLLVASFLVVLGICVGLGLLGATSFANGLHFGLSLLAAVIALLALRTGVQAAL
                     LHEAHDEMNPQAQVLCPHCDHVVPDTAFCPNCGVATRAASRTSRAARRTTQGVTSARP
                     GYAVPPGSYAAVPVQHTTHTKLLTALGAGAAVAVAAAVTASVLATPVAARYVCPPDCG
                     EPPIGKPVAANPWFTSGDGKFAVQYPRAGTAYEVKLESEGVEVNFTAGDTGTMELFGM
                     PASNRTPKQIAEALINEHYPDATTDYEIPNAMVGYQPGYGVVKDEYPQDASGTFTRLR
                     LLVMVAVKNDYALVAAAIGPYHEFSPDFGSGHPSGVNLQLALDMGKYVNSFTWRDDSP
                     R"
     gene            complement(29652..>29976)
                     /locus_tag="VFC01_06970"
     CDS             complement(29652..>29976)
                     /locus_tag="VFC01_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79416.1"
                     /translation="VEQAPPQRIPVAPPSPPAAPPLEPAPPVAQTIVEAAAPVPDAIP
                     VVPPPEAPVNSEPPVAPPPAPAYVPPAPAPNYVPPVPQQPRLRDRIIERIPIINRFHE
                     PQPYG"
BASE COUNT         5032 a         9774 c         9901 g         5269 t
ORIGIN      
        1 gcctccaaca tcggttcatc cggtttcccc agcgtcggca gctgatagaa gtgatccatc
       61 accaacacgg tgtcgaatcc ggcggattcg gcttcctggg cttgcgcctt caccgcggga
      121 aacagctcgg cgcctcgctg atcatctccg cgagacgggc tggaacatca ctccagtcga
      181 tacggccgat gcccccgtgc gacgcgacgc gaaggagacc tggcgcggcc ttcgcgattc
      241 gtccggctac gtcgccgcct atcgcgtcga cggcgatttg ctgaggttcg ggcgttgccg
      301 tcgtcggaga tctggaccgc cctcgagatg actggcacgg cagatgattt gcgtaccgcc
      361 gcggcgtgcg ccatccggac ccacgacaag cccgaggccc ccgctggcgc gacgccgatg
      421 aacggacgcc atcgccctgc cctcgacgcg ctgagcccga tgtcagtcga gcggctcact
      481 tgaacatgaa atcacgcatg aaccgggtca tccggcgcaa atagtccggc tggctgacgt
      541 gtaacacgtg attgcctggg aaccaatgga aagcgcagcg atcccagtgc tcccacagca
      601 actccgcctg ctccggcggc gccagccgat cgccgagacc ggtgatgatc agccgccgat
      661 ccttgggcac tagcggcgga tagttcagcg gcgacgggta tttcgtcgct tcgtgcacca
      721 actcgtcttc ggcgccggcg atcaggtcgc gcaagcgcac cacatagttg gcggggaacc
      781 actcgtcgac ggtcctgtcc ggcgtcacga ccggaacgtt ggggatcacg gcctggatgc
      841 ggtcctcgac gcacgcgatc agcgccgacg tgtatccgcc cagcgacatg ccggtcagcg
      901 ccacccggtc cacacccgtg aactccagat agtcgatcag cgaacggaag tcgtgcacgg
      961 cctgcgccat cgcttcagca aaacccgcaa agccgtgcgc gaagtagccg taaccactga
     1021 agggagagcc cttttcggca cgcgccccat ggaatggcaa cgtgtacaac agcacgtcat
     1081 aaccggatcg gtagaaccac ggcagcgaga agaacaaacc gttgaacagg tatggcgatc
     1141 ccatgaaccc gtgaatcaca cacaacgtgg gatgtgggcc gtcgtcgtgc acccagtgtt
     1201 gcgcgtgcac cacgttgttg cggacatagc cgcgacactg ctcccgtagc gcggggttga
     1261 ccgcctcgaa gctgctgtcg aagcggatgt tgcgaacgct gcctttggcc atccactcgg
     1321 cgatgggatt ggccgggcgc gacgagatcc gcggctccga cgtcggcgcc gggaaggaca
     1381 actccggatc cctcgcactg gccagttcgg cgtagaaccg cagatagtca cgttcagcgc
     1441 gtgacgcgga ccggcgcagc gcagtgccga gcatcgtcgg caccatcgcg acactcagca
     1501 gcgatgcgat cgcggtgcgc agcgcgatgt cggccatggc cgacgaatcg acgatcaacc
     1561 gttgacgagg cgtgaggtcg acgcggcggg ggagtccacc ggcggtggcg tcggcgccgg
     1621 gaacgtcggg aacagggatc ggcggatcga cgggcgcgcc gtcggagtcc cgcgaactgg
     1681 acacatccgc atggtagcgg tcagatcaag cggtacaccg ttgatccgcc gatgaacgtg
     1741 cctggatagt gcgcggtcac ccagtcggcg atctcgcggg tgtgcgacgc ggtgtgcagt
     1801 tcgctgcgga tgacctcgcc atggacgccc ggtgaattcg gtccgcgacc ggcctcgacg
     1861 tagtacgaga tcttgccggc gtgcacgtcg tcgatgaact gctgcagagt cggcaccggg
     1921 tccccgctcc agccgccgac ggccatcacc gatgtaccgg aggcgatttc gagggaggca
     1981 gcggactgtg agccgttcgt cgccgcggac cactgcgtgt gcgtggcggc cagtacgccg
     2041 gcgagttggg tattggtcgc ctcgtcgttg ccccaggcgc cggtcacctg cgacgagcgc
     2101 accgcggtgg gtatcgtccc gtgatgcggg gttgcggcgg tcgcgacgga aaacactgtg
     2161 gtgccggcca atccggtcaa gcatccggat accagcaggg cgatcggtgg acgcggccac
     2221 aacactgcac tgccgctcac cacagccagc acgacgatca gccacggtag ccacgaatgc
     2281 ccaaaggcat ggcggtgcag caagaccgat gacgggacag cagccagcac gatcatcgcg
     2341 gccagcacgc accggccgtc ccagttcgaa cgtcgctgcc acgcccacac cgcaccgatg
     2401 cccaccagtg cggcgatcgc aggagccagt gcgaccgtgt agtacgggtg gaccatgccg
     2461 cccatgaagc tgaacaccac accggtcacc gtgagccatg cgccccacat cagcagcgcg
     2521 cataactcgc tgcggctcaa ggcccgacgc gcggccaagt acatcccgaa tgccacgacg
     2581 aagaacgcca cggggatcag ccatgagatt tcattggcca tctcgccggt gaacagtcgg
     2641 tgcaggtcgg tgcgcccgcc catcatgggc aggctcgaac tgccccagtc gcccaacggt
     2701 tttccctcat ggtggcgccc caggatgcgg tcgatcccgt tgtagcccaa cgccagatcc
     2761 agcacggtat tgtctttcga cccgccgatg tacggccgcg cgctcgccgg tgtcaactgc
     2821 accgccagca cccaccagcc ggcagccgcg atcaatgctg cgacggcgcc gagcagatgg
     2881 gccagacgct tgccccacga cgtcggcgca acgcacaggt acgcaagccc aaagccaggc
     2941 agcaccagga agccttgcag catcttggtg agaaacgcgg tgcccatgac cacaccggcg
     3001 agcaccagcc agcgccacga cgctgccatc gctgcgcgca tcaggcagta ggcggccacc
     3061 gtcagcaaca gcaccagcag cgcatcgggg ttgttgaagc ggaacatcaa cgccgctgcg
     3121 ggcgtcaccg ccagcacggc accagctagc agccccgcgg ccgcaccgtg gttcggattc
     3181 gggactgcgc gtcgcaccgc gcaatacagc acggcgacag cggcaacgcc catcagcgcc
     3241 tgcggcgcca ttaccgacca gctgttcatg ccgaacaagc gcaccgacag cccggtaacc
     3301 cacagagacg ctggcggctt gtcgaccgtg atgaagttct gggcgtccag cgatccgaag
     3361 aaccacgctg accaactctg tgaacccgcc tgggctgccg cggcgtagaa ggtgttgccg
     3421 tacccgctgg ccgacaggtt ccacaggtaa agcgcagccg tcgccgcgag cagggtgccc
     3481 aacgtccatc gcgaccagtg gccggtgcgg gccggcgcga tggtctctac ggagaccggt
     3541 tggtcgacga gggcagtcac gacgcatacg gtgccgttgc ttaccagccg cgggcctgtg
     3601 ccagggctgc gaacacgctg tgagttcgat ggtggtgcga cactggccgt atgtggaatc
     3661 ccgacgtcta cctgaccttc gccgaccatc gcggtcgtcc gttcttcgac ctgttgtcgc
     3721 gggtcggtgc cgagaagccg cgctgggtgg tcgacctcgg ctgtggtccc ggcaatctca
     3781 ccgcaaccct tgcgcagcgc tggcccgata ccgtcatcga agcgtgggac agctcaccgg
     3841 agatggtgga ggccgcccgc gaacgcgggg tggatgcgca catcggcgac gtgcgagcgt
     3901 ggacgccgcg ccccgccacc gacgtcgtgg tgagcaatgc caccttgcag tgggtgccgg
     3961 agcacgccga gctgctggtc cggtgggcgg accagctcga gtccggttcg tggatcgcca
     4021 tgcaggtgcc gggaaacttc gacgcgccgt ctcatcgggc cgtccgcgaa ctcgcccgcc
     4081 gcgaccaatg gtcagagccg ttgcgggact tcccgtttcg cgagggccag gtcgacgatc
     4141 cgtcaggata cgctgggctg ctcagcgacg caggctgcac cgtcgacgcg tgggagacca
     4201 cctacatcca cgagctgact ggtgacaatc cggtgctgga gtggatcacg ggcaccgcgt
     4261 tgacaccggt aaagagccgg ctcaatgagg aacagtggga gcagtttcgg caggagctga
     4321 tcccgatgct gcacgcggcc tatccgggtc ggcccgacgg cacgacgttc ttcccgttcc
     4381 gccggatatt cgttgtcgct caggtacgtt gacgtctttc gttgagcaga tcgatgactt
     4441 gcgcgacgag ctggttgatg tccgcgccgg tggtgtcgag cacaagatcg ggatcctcgg
     4501 gcgcctcata gggcgcgtcg acaccggtga gacccttgag ctcacccgca cgggcccgcg
     4561 cgtacagacc cttggggtca cgccgttcgc attctgcgcg cggcgtgcac acgtacacct
     4621 cgatgaacgg cagcctggcc gcattgttga gcgcgcgggc gatctcgcgg tcggacttca
     4681 gcggcgacac cagtgacgcc aacgcgacga cgcctgcgtc ggcgaagagc cgggtgagat
     4741 ggcctacccg acggatgttt tcggcccgat caccggccga aaagccgaga tcgtccgaca
     4801 ggccgtgccg gatgttgtcg ccgtcgagca gatacgcgac ctgaccggac tccacgagtg
     4861 cacgctcgac cgccaccgcg atcgtcgact tgcccgacgc cggaagcccg gtgaaccaga
     4921 tcgtggctcc gtgttggccg gtggccgtcc agcgatattc gcgatccaac gacgaagggt
     4981 gccagcggat gtcgttgcgg gtctgcaccc ccggctggac ctcgcgtggc tcgaggatcg
     5041 tcccagcgcc gacggtgtcg ttggtggtct catcgatcag aatgaacgcg ccgctgtcgc
     5101 ggttgtcggt ataggcgtcg gcgacgacga tcgaactcgt ccgaagcgtc acctcaccga
     5161 tgtcgttgag caccaactcg accgggttgt ccaactcatc gagcgtttcg gggtccagcc
     5221 tgctgtgcag ggcctgcact gtggtgcgta cggttcgggt gccctgcttc agcgccagcc
     5281 ggtcgcccgc ccgcaacgga ctgtcgatga accagcacac cgtggcgtcg agctcgcggg
     5341 ccagcacggg atgtgtcgcg tcatcggcgg cgctgacgaa cacatcacca cggccgacgt
     5401 cgatgtcgtc agccagctcg atcgaaaccg atagtggggc aacggctgtg gtgcgctcgt
     5461 catcgagggc gtcgacgacg gtgacggacg accgggtgcc tgcgggcagg ctgatgaccg
     5521 ggtcgcccac ctggagtgtc ccgccggaga gccgaccggt gtagcggcgg cgctggtcgg
     5581 ccgtcggccg cgaaacccac tgcacgggca accgcaattt gctcggttcc ggttgcggcg
     5641 cggtcagttc gacgctttcc aggtactcga gcaaggtggg cccctgatac cagggggtgc
     5701 ggtcggatcg gtgcacgaca ttgtcaccgt gcttggccgc gaccgggata acggtgatgt
     5761 cgacaccacc gaggcgcgcc gccgccaatc gcagttcgtt ctccacctcg gtgaaccggg
     5821 catggtcgaa gtcgacgagg tcgatcttgt tcaccgtggc gacgaagtgg gtgatgccga
     5881 gaagttttgc gattcgggcg tgccggttcg tttgacgcag cacaccggca cgggcgtcga
     5941 ccagtagcac cgcaacgtgg gcgttggaag cgccggtgaa catgttgcgc gtatagcgtt
     6001 cgtggccggg cgtgtccgcc aggatgtagc tgcgggtgtc ggtggagaag aaccggtacg
     6061 cgacgtcgat ggtgatgccc tgctcccgct cggcgcgcag gccgtcggac agggcggcga
     6121 ggtcggcgac gccttcctca tcggtgaccg cttcgaggtg gtccaacggg agactgtcgg
     6181 tgtcgtgcag cagtcggccg atcagggtgc tcttgccgtc gtcgacggag cccgcggtgg
     6241 tgatccgcag cagctgacgg gtgatcgtct tgggtggtgc cgtcatcaga agtacccctc
     6301 gcgcttgcgg tcttccattg ccgcggcaga cgttcggtcg tcggcgcggg tttcgccgcg
     6361 ttcggagacg gtggccgcgg agatctcggc gatcacccga tcgatgtcgg tggcttctga
     6421 gcgcaccgca ccggtgatgg tgagatcgcc gacggtgcga taacgcaccc attcgaccgc
     6481 agcgatttca ccgttgaccg gcttggcgta ctcggagacc gcgagcagta tgccgtcgcg
     6541 ctcaaacacc tcgcgttcgt gtgcgaagta aatcgacggc agctcaaggt tttccaactc
     6601 gatgtagcgc cagacatcga gctcggtcca gttgctcaac gggaataccc tgacctgttc
     6661 acccttcttg atccggccgt tgtacagcga ccacggctcg gggcgctgtg cgcggggatc
     6721 ccactggccg aactcgtcgc ggaagctcag gatgcgctcc ttggcgcggg cgcgctcctc
     6781 gtcgcggcga gcgccgccga aagccgcgtc gaattcgcct gcctccaaag cgtccaacag
     6841 tgtgcgggtc tgctggcggt tgcgggacgc gccggggccc ggatcaggta cgcggccgat
     6901 atcgatggac tcctgcacgg acgcgacgat cagcttgtgg ccctgaccgg tgacccgccg
     6961 gtcccggaac tcgatgacct cggggaagtt gtgcccggtg tcgacgtgca tcaccgggaa
     7021 cggcagcgga ttcggtcgaa aagccttctc cgccaggcgc agaagcacga tcgaatcctt
     7081 gccggccgag aacagcagca ccggccgttc cagttcggcg acgacctcgc ggatgatgtg
     7141 cacggcctct gcttcgagca gccgaagctc gtcgacgtgc accgtgtcga aggcggttgt
     7201 catgttggga gtccctccct ttccgcgtcg gcccggtagc ggtccaccca ttcgtcggag
     7261 cgctgatcgg cctggcgttc cagcaccggg gccggcgcca gccccggatc ctgaccgagc
     7321 gcctcgagaa cggtgccgac gatttgggtg aggttgcgcc acaacaccgg ataggccact
     7381 tccattggct cgacgccctc cgctgcgaac cacttgcgcc aaccttcttc ttgtgcgcgc
     7441 atcatggaca ccacatgagc gatcgcgccg gcgtggtatt cggcgcggga gtcgcgggcg
     7501 gggtcgggtc tgccacgcca gacgcgggtc tgcactgcgc gccagaacga aaccgcttgg
     7561 gagaccatgt cgggtcgata gacgtggatg agcacgggat cgctgccgat gacatcacgg
     7621 atcgcggata gcagcccatc gccggagcga ccgggcaagc ccgcggcgcg ctgcagcagc
     7681 agcggtgtct ggttccacat cagcttgccg ccccagatgc cgtttggggt gcgaccgacg
     7741 gtgcggatgt agtcgcgcca gatttcgggc ggcgcgaggt cgggcttgcc ttcgtcgagc
     7801 ggatcgagta gccgaaggat cgattggtcc tccacgcctt cgaaccattg cctcggctgc
     7861 ggcgattggc ttgtcgtcgg caggtattgg aagaactctc ctggctcgcc ggccacaccc
     7921 gtggcccgca acgattcgac gagcagcgtg ctgccactgc gctgcgaagc tagcaccaga
     7981 taggccgtcg agtgggacat gcgcgtgagc ctaaccgcaa tcaaatcgct tggcactgac
     8041 gtacattgca atcaccgtga gcagaactat tgtccggaac tgtggtcgtc ttcgatgcct
     8101 cgttcggagg cgatggccga gcgactggtg acctgtggac cgtgaattcg taggtaggtt
     8161 tctgtgtacc gttcggcaat tcgggtaccg gcgaagtcgg acggtatgac gtcgttggtc
     8221 ttgtggcgcc agtcgtcgag gagcagggcg aggtcgttgg cgatcgcttc ggccttgtcc
     8281 gtgacgtcag gccccagcag gttgtgcgac tcggtgggat cctcgacaag gtcgtacagt
     8341 tcgcgctcgg gccgctgcgc gagtacgagc ggctccatga tgcgaccggg tgcgctttcg
     8401 gcgatgtccc acggcagatc gagaagcggg cgtgcggcgt agttctcgat gtagctgtat
     8461 tccttcgtgc ggatcgcgcg aatcgggtcg aacgaatcgt ggtaggtctt cgtggtgtag
     8521 acctcggtgc gcaccgcctc ttggggtgag atctgcagat tgcgggcatg cgacaagccg
     8581 tcgatgtcgg tgggtatgtc gatgcccagt agctcgagga gagtcggcac gagatcgaca
     8641 ccgctgaaca ggtcgtcgta cacctgcggc tcgatgcggg tgcgcctcgg tggccgcacg
     8701 atcagcgcga ttccggtgcc ggcgtcataa agtgtcgatt tggcgcgggg cagcgccggg
     8761 ccgtggtcgg tcatgaagac cacccaggtg gattcgtcga ggcccgttgc gtccagggtg
     8821 tcgagcagtt ggccaacggc ggcgtcggcg accgagatgg acccgtagaa ctcggccagg
     8881 tcctgccgga tctcagcgtt gtcgggcagg tagtcgggca ccgcgacgtc atcggcgtcg
     8941 gcgggctcgt agcggtcgcg cggatacggg cggtgcgttt cgaagaagcc cgcggtgagc
     9001 aggaacgact gctcgggagg gtcggtaagc cagcgggtgg cgtgttcaac gacgtattcg
     9061 cagtaggagt tcgaaacatc gaactcctca aagcccaggt tcgacgggta cgacgtctcg
     9121 tgctgcatac cgaatagtgc tgtgtaccaa ccagattcga tcaaaatatg gggcagcgtc
     9181 cggacgccgg cgcggtactc ccagccgtga tgggccaacc cgagcaggcc gttgttctgc
     9241 gggtagcggc cggtgaacag ggagccgcgc gacggggagc acagcggcgc ggtggcgtgc
     9301 gcgcgagtga agagaatgcc ctctgtggcg agccgatcga gtcgtgggct ggacacgtcg
     9361 gcgcggccgt aggcgccgag gtagcggccg aggtcgtgcc aatgcacgat cagcacgttg
     9421 tcccgtcgct cggtcactgc ctgtcctttc gcgtcccgtc ggtacaccga acagtgccta
     9481 tgaggattcg gcaacccttc tgctcacgca gagcaactta tccccagttg cgaattcatc
     9541 cacaggttgg gctgagcggc ggctttgggt agggctgggg tcgggtggcg ccggtagttt
     9601 cgctcgtatg ttcgatggtt cgttgccgga ggtccgcgag ttagccgcgt tggaggacgc
     9661 ggcgctcgtc gatgctgccg ccggctgggc gcgcaccgag aacgccgcgt gcgcgcgcaa
     9721 gacggcggtg atggccgaac tgttcgcccg gcgcaccgga ctgcccgccg gcgagcgcga
     9781 gctgtggtgg gtcgacccgg aggcgagtgt cggtgcggag ttggcggcca gccagaacat
     9841 cagcacctgg atggcgctgg cccaagctca tcgcggggtg gtgctggccg atcggctgcc
     9901 caaggtggcc gctctgtttg cggccggtct gatcagtgag gtgctggtgc gcaccatcga
     9961 gtaccgcacc gacctggtca ccgacgacga ggcgatcgcc cgggtcgatg tcttggtggc
    10021 cgagcacgtc accgcctggg ggccactgtc ggccaagaag ctcgaacagg ccattgatgc
    10081 catcgttgat caggtcgatc cgggcgccct gcggcgctca cgcaagaccg cgtgccagcg
    10141 ggatgtgcaa ttcgggtcgc cctcggatga ggccggcttc accagcatgt gggcccggtt
    10201 gtatgccccg gatgcggcgg tgctacagca gcgggtggac gagatggccc gcggggtgtg
    10261 ccaggacgat ccgcgcacgc tgggcgagcg gcgcagtgac gtgttgaccg cgctcgccac
    10321 cggcatcacg cagttggcct gcggatgcgg cagcaccgac tgcccggccg ccgcacccga
    10381 cgccacgccg gcgacgcccg cggtcatcca tatggtcgcc cacgccgaca ccgtcgatgc
    10441 cgcccagacc caaaacacca caccaccacc cgacgccaac gggcctgacg ctgatcctgc
    10501 gccggctccc ggacctgacc tcgacaagtt ggcgtcgtca gagcctgtcg acgacgaccc
    10561 cgctccggcg gcagacgccg cgggtgtgcc gccggccttc tgcccggccc cggtcgccga
    10621 aagtgcctcg gccccaccgg ccttctgccc cgcgccgccg gcgttcgtca tcggtggcgg
    10681 ggtgctgccc gcgccgctgc tggccgccgc gctggagcgc gccacggtgc gcgagatccg
    10741 ccaccccggc gatgcgccag ccgaacggca ctaccggccc tcacgagaac tggccgattt
    10801 tgtgcggtgc cgcgatctga cctgtcgctg gccgggatgc gacaaacccg cctacggctg
    10861 cgacatcgac cacaccgtgc cctacccgat cgggcccacg catgcctcaa ataccaagtg
    10921 ttactgccgc tttcaccacc tattgaaaac cttcctgtgc ggtggggacg gctggcgcga
    10981 gcaacaactg cccgacggca ccctcatcgt cacctcacca accggacaca cctacaccac
    11041 caaacccggc agcgccgtgc tgtttcccgc actatgccga cccaccggca ccctgtgggc
    11101 acccggagaa gaacccaaag tctggccccg cggcgaccgc ggactcatga tgcccaaacg
    11161 ccgacgcacc cgagcccaaa acctcgccaa acgcatcgaa gccgaacgca aactcaacga
    11221 cgactacatc gccgaacgca acaaaccccc acccttctaa cggtttcgca aacctacggg
    11281 tggtacggga cgccgaccgc gcggaacaca tactcgggcg gcacatcgaa cgccagggat
    11341 cgacggggca gccgcatcag gacgtcctca ggcagtgcat ccttgtagtg caacgagagc
    11401 tggccggtgc agcgcgggtc gacatcggcg acgttggcgt cgagcaccag gccggtttcg
    11461 gacagctggg cctttacggc gcccgtctgc ggtgcgtccc agcgcagatc aatggtgcgg
    11521 cccgccaact tcggcaccga cgagagtgtc gccagcaccc gttggttcgt gagcaccagc
    11581 gagccgacgt agcttgcgat gctgcccgca gaccgcttgc ccgggacccg tccgctgaac
    11641 cgccgcgtca ccggaacgta ttcggcaatg tggatgatgc cctcggcctc gacctcggtg
    11701 cgtatctcat cgggaagctt gccgatcccc agcaacttcc gcagaaagac agccatgtgg
    11761 agtaacccta acgggacctt gcagcgtcgc gggcgataca ggtggtagag ctggtgagat
    11821 gacaccgcgc aagacctggc tgatcatatc ggcggccgct gccgtcgcgg tctatgcgct
    11881 gatgtggatc ggctatgcgt cgcactggaa ctggctgacg actatcgact cgttatggct
    11941 ggaggccacg catcgctacg gcgcccctcg tccgggatgg gtgaccgcct ggaatgtgtt
    12001 ctgcaccgta ctcggcccga ccgcgttccg actcgcgacc gtcgcggtca tcatctcttc
    12061 cctcgtgcgc cgcaacgttc gcgtcgcgat gttcctggtg atcagtgtcg agctggccgg
    12121 gctcgtcacc gagatcgcca aatacgcggc ggatcgtccg cgccccatca ccgcattggc
    12181 ttccgcaccc tcgacctcgt ttccgtctgg acacgccctg ggtgtgatgg tcggggtgct
    12241 ggcgctgctg accgtcgttc tgcccgtcgt tcgccagcca ctgcgagcat tgctcgtcgc
    12301 atgtggtgcg gcggtcgtcg tcctgatcgg agtcggccgt gtggtactca acgtccacta
    12361 tccgtccgat gtgctcgccg gatgggcgct gggctacgca tatttcgttg cgtgcctgtt
    12421 gatggtcccg ccgtcgcggc ctgtcacggt aacggacgaa ataccggcag cgctcggtac
    12481 ttcgaggtga aggtggcctc cgtgacctcc gtgccgacct caccgtcatg tgccagcacc
    12541 gtcgggccgt ccaccgtctt gaaggtgaac tcgggcaccc gcagctcgtg atacagcggg
    12601 ctgcgctcca ggcgacccag tgctagcgcc gccacaatcc gcaccctgct gaaccgccgg
    12661 cccgtctcta gaatccggac gtccagcagc ccgtcgtcca ttcgggtccg ccgcgacggc
    12721 gcgaaccccg acggcagata cgtcgaattg ccgaggaaga acagcgaagt ctgcagcgtc
    12781 ttgttgtcgt acacgatgcg caccggccgg tcctgctcca gcgtgcgcag catcgcgtac
    12841 aggcccgcca gcggcttgcc gaccttgtgc tcgagcttct cgcgcgtccg cacgaatgtc
    12901 gggtatgcac cgatgcttgc ggtgttgatc accatgcgac cctcgttgag gcacaccata
    12961 tcgacgcatg ccacgctgcc ccgctggatt gcctcgaccg tcttgtcgac actgtcgcag
    13021 ccgatgtcct ttgcgaaatg gttgaaggtg ccgccgggga agacggccag cggcacaccg
    13081 gcttccgctg ccacaccagc tgcgcacgac accgtcccgt cgccgccgcc gaccgcgagc
    13141 acctcggccc gctcgaccgc cttgcggagt acttcttcga catcgtcgtc ctcgcccagt
    13201 tcgacaatct cggtctgcgg caacgactct cgcacttcgt cggcgacccg cgcgcccgtc
    13261 ccgccgcctg acgcggggtt gaccacaagc accacgcctg cgccgtcggg acgttccggg
    13321 gtatccacgc gcagcgggtc cgcgaccgga atcttggctg tcacaaccgg cggcaccacc
    13381 cgcccgccca gcaccgcgat gcctgcgccg atgccgaaac cggcgaacac gtcaccggga
    13441 tagtgcaccc cggtggccac ccgggacagt ccgaccaagc cggccagcag cgccagcccg
    13501 agtcccaacg gtggcttttc aagaccgacg ccgaccgcga aagccgctgc gctcgccgag
    13561 tgacccgacg gcagggaatt cgacgtcggc gatcgatggc ctcgccgcgc gatcggcacc
    13621 gacaggcggt tcggcctcgg ccgacgccac ccccgtttgg ccaccagatt ggtaaagagg
    13681 ctggtcgccg ccagggagag caccccacgc gttgcgctac gccgcgccga cggaccgccg
    13741 agcactgcaa tcaccgcggc gagggccagc cagaccttcg aatggtccgc ggccctggtg
    13801 agccgcggca tcgtggcgtc cagcagcggg ctcggtgact ccgcgatcgc ctcgaacacc
    13861 tcgcggtcca acgtgcccag accttcgcca atttgccgga taccgcgcag gcgccctcgc
    13921 aagggcagta attccatgac gtctacgtag ccagatctga cgttcctcaa tcgtgtctga
    13981 acctttactt gaccccggct gtgacaccgc tgacgataag tcggtgcgcc gactgctggc
    14041 tctgctgtgc gccggagccg tctcactgac cgcggcaccg gtcgctcacg ccgagttcac
    14101 gccgtggttc gccccgcagg tcggtaacgc cactcaggtg atttccgttg tggggtctgg
    14161 tggttcattg gcgaaaatcg acgtcttcca acgcggcgcc gccggatggg aagccgtgcg
    14221 cgcgggcatc cccgcccatg tcggcgccaa cggttttgcc gccgagaccc acgacggcaa
    14281 catggcgacc ccgctgggca tttacaccct ggatttcgcc ttcggcaccg cgccgaatcc
    14341 gggcggcggc ctgcaatacg tccaggtcgg tcccgaccac tggtgggacg gcgacatgaa
    14401 gagcccgacc tacaacacca tgcaggtctg caagaagtcg cagtgtccgt tcgacacgtc
    14461 accggacagc ggcaccgaga acctcgagat tccgcaatac gcccatgccg tcgtgatggg
    14521 cgtcaacaag gcccgcgtac caggcaacgg cggcgcgttc ttcctgcaca ccaccgacgg
    14581 cggtccgacc gcgggatgtg tggcgatcga tgacggcacc ctggtggaga tcatgcgctg
    14641 gctgcggccg ggtgcgctga tcgcggtcac ccagtaatcg tcaaatatta agcgcccgac
    14701 cggttttcag cttgaagttg aggttctccg tcgacatggt cgcgtcatag gtgcggtcgt
    14761 agcccgtctt gctgatcttc cagccgtcgc cggtcttgcg gtattggtcg tggtagaagg
    14821 cggcgccaat caacatgaaa ttgaaatcgg gcacgatcac gcggtcctgc agataccaag
    14881 tccccgacgc ttcgttgccg ttcaccgtga tctcgggatg ggtgacgcgg tgttcggtga
    14941 tgatgttggc tggcatcgac gtgcgcatga actcgacgag cgtgtcccgg tcggtgaagt
    15001 ggtgtgactc gccgattgat gcgtcgtagt cgccgatgat gtcttcggtc agcgtgtcgg
    15061 cgaagtcgtc ccagtgcttg gtgtcgagcg cacgcaggta ccggtatttg acttgctgga
    15121 tctcgtcgat gtcacccatg gccgacattg caacacagcc gggcacaacc gcatagagtc
    15181 cggtcatgaa attcattgac agctacgtca gatccccctt cgcggggatc gcaccgtggg
    15241 tgctgatggg tgtggtgtcc gggccgggcc gattcgagga agccgcctcc gccgcgttgg
    15301 gcctggcgct gctgaccctg tgggtcggct ccaggcgcgg cgtccccgtg caccttctcg
    15361 aggtgttcac cgtcggctac ttcggtgtgc tggcggtgct cgggctggtc gcctcggacg
    15421 ccaccatcga ctggctggaa ctgtgggccg gtgaactaag caatatcgcg ctggccgcgt
    15481 tcgcgatcgt gactctgctg atccgcaagc cgttcacgct ggcgtacgcg aaggacacga
    15541 cgccgcgcga gcactgggac gatccgctgt tcctgcggat caactacgtg atctccgccg
    15601 tgtgggccgg agcgttcctg gtctcggccg tcgtcggcgc ctacggcgat gcagtattgc
    15661 gcgacaacga caacttctgg accgcatgga tcgtgccgct ggcggcggtc atcttcgcgg
    15721 gcgcgttcac cgagttctac cccgaccgcg cgacgggtga agcgacgtca tgggcccgga
    15781 tacttgactg gctgccaccg ttcgtgctcg tgaccggcat cgtcggatgg gtgtcggacg
    15841 ctgtgcccga cggggtcggg atcgggttga tcgtcgttgg catcgtcggc tcggccgcga
    15901 tgcggaagct gttcccggcg caggcggaag ctactgagcc gctgtgagat tgcgtgtcgc
    15961 cggaccctcg agcagcgtgc cgtccggcgc gaagcgtgag ccgtgcagcg ggcagtccca
    16021 cgattcgtcg gcgtcgttcc agttgacgat cccaccgagg tgcggacaca ccggcgatac
    16081 ctgatgctcc acgccgttga ccacgcagcg cgcctccaga tcccacggtg ggccgctgac
    16141 caccccacca ctctccggtg tccggtgggc tattcgaagc accggcgtga tccacccctt
    16201 ggccaggttg aacccgactt cgaggttggc ctgcaacgct ttcggaatgc ccgacagctc
    16261 atgcgggctc cagctggcga acgcgttcgc ccagtccatc cgcccgccga ggattcgact
    16321 cgacaatgcc agcgcggcag cggtgccgtt ggtcattccc cacttgtcga aacccgttgc
    16381 aacgaagatc ttttcgttac ccggcaggat cgggccgacg tatggcaact cgtcggccgg
    16441 cgtgtaatcc tgggccgacc agtagtgcgt ctgcatcgca cccgggtagt tcttcttggc
    16501 ccacgaatcc agttcctgca ccgaacatgc cgggctcttt tcgcggccca ccgggtggcc
    16561 cgcaccgccg acgaccaacc gatcaccacc gggcgtcggc gcgtaacgca ccgatcgggt
    16621 gggcgagtcg gcggacaggt acatgccgtg ggtgatgttg cccggcacct tgtaggccat
    16681 gcagtacgag cggctgggct tcaaccgcgc gaagaagccg ccgcgatcga ggatcggaat
    16741 gccggtcgcc aacacgcatt gcttggcgtg cacgtcgaat tcgtcaccgt cggtggtccg
    16801 tacgtgcaat gcgagcgcgt cgtccttgcc cgaaacgctt tgcacgcgca cgctttgcgc
    16861 gatccggccg ccgcgctctt cgagctcgac gatcaaactg tcgagcagcg gcatcggatc
    16921 gaactgcgcc tgatgcgcga gccggacgcc gccgtgatac gggaacggca catcggcgtc
    16981 gtccgcccat tcgacctcta agccagcagc cgtgcacgcc tccagttcgg cgcgggcggc
    17041 ggcgacgccg tcttctgatt gcgcgtaggt gtaggcggct tcatgctgca cggagatgcc
    17101 gtgcgcctcg caatgctgga ccaaccactc ctggccctcg cggttgccct gtacatactg
    17161 cgccgcggtc ttcgggccat gcttgccgac gattttcgac atcttcgtgc cctgcagcag
    17221 gctgatcttc gcggttgtgt tgccggtggc gccagcacct gcggagtgtg cctccagcac
    17281 gagaacgtcc ttgccggccc tggccagcaa caccgcggtg gtcaacccgg tgagcccggc
    17341 accgaccacc acgacgtcgg ccgaaaggtc cgcttcaagc agcgggttcg gcggtatcga
    17401 ccggtcgatc cggtcggcga gccacagcga tgtcatggcc atgctgtacc caggccagca
    17461 aggacgaaac ccctcgttaa ccccagtagt cgtggtcgtg gttgtcgccg tcaccgtcgc
    17521 gatggtgatc gtcgtggcag gtgttccagt cgaagttgaa accccactcc ggccgccaga
    17581 attcgccggg gcaccagtga taacgcgggg catcaaaccg cacgcctaca ccgaaatcgc
    17641 ggtggtgatc gtcgccgtca atatcgcggt gacggtcgtc atggcaggtg cccgcatccc
    17701 agttgttgcc ccatttcggg atgccaatcc tcacccggac accagtggta gctcgggaat
    17761 tcgcccacct gggctgtcgc ggcggttgcc gctcctacac cgaccaatcc gagcgcggtc
    17821 gtcatgatga atgttgttgc tgcgcagcgc gccaatgttt tcacgcgtac tacgacaaca
    17881 ctgaaatctg tgtcccagct atgggcttga gtccaaccgg ctggcaatgc ggcccaaaaa
    17941 ccggagtgtc taacggaatt ggtctcgtcc ggcagctatc gtgtggtccg ttatgagcga
    18001 cccgttgggg ttgtcgatcg ggacgaccaa cttggtcgcg gcgcgtgtcg gcaatcaacc
    18061 cgtgttacgg cgttcggtgt tgaccctgtt tggccatgcc gcgccggaag tcggcgtgcc
    18121 ctcagcacac tccgacggca tcgtgctggg tggcttcgtg gagcgcgtcg gcgaccccgt
    18181 cccgctggtg gccgccgacg ggtcgccgca tcaggccgac cagctgttgg tcgatgcgct
    18241 gcaggcgatg gtctacgcca gcggcggcca gccgtcgccg gagaccgcga ttgcggtccc
    18301 cgcgcactgg agcacatcga cgctgtgggc gctgcgcaac gcgatgcgcg cgagtccgac
    18361 cctggcgccc aacggcatgc cggtacggct ggtctccgat gccgtcgcct cgctgacggc
    18421 gctgaacgcc aatcccggat tggtttcgca gggtgtggtg gcgctgctgg atttcggcgg
    18481 cggcggcacg aacatcacgt tggcggacgc cgcatcgcgg tacgagccga tcgaagacat
    18541 cacgcgatac gccgaatttt ccggcgacct catcgatcag gcgctgctga gccatgtgct
    18601 ggagggcatc gccaactcgg gcagcgtcga tccggcaggc accgcggcgg tcggttccct
    18661 ggcccggctg cgcgaggagt gccgcaacgc caaggagcgg ctgtcggcgg agacggtcac
    18721 cgaactgatc gccgaactgc ccgggtaccg ctcggggatc cgagtgaccc gcaccgaatt
    18781 ggaaagcctt atcgctccgc cgcttaccgg ggtgctcacc gctctggaaa atgcgttgga
    18841 gcgcaacagg attagctggt cgaatgtctc tgcggtggtc accatcggtg gtggtgccag
    18901 cattccgctc atcacagcgc gactttccga acacgcgcag gcgccggtgc tgacgacgcc
    18961 ccagccggcg ctggatgctg cggttggtgc ggccttgttc gccgcctacg gtgcgcaagc
    19021 cgacgcgaaa accggggtag ccccggcgct gccggtggat gttcccgatc cggtcgacgc
    19081 gccgggctcg gcgacgttcc gtgccttggc ttggtcccag gacgacgaca ccgaggacgt
    19141 cgtgccgtac accggagatg tgtacgacac cggcgtgacc gccgcgcgtc cgcaggtgca
    19201 gtacatgccg gccaccgggc cgatcgagga gccgcgttcg acatggaatc gggtgccgca
    19261 gttggtgttc gggctcgcgg ccgtggtcgc ggtggtggct gttggcgggg tcgccatcgc
    19321 cctgacgagc gccaccgaca gcaccaaggc caccgagccg acgaaacccg ccacctctgc
    19381 ccccgcaccc accagcgcgg caccgccgcc gtcgactccg ccacctgtcg agaccgtcac
    19441 ggtcaccagc gagccgccac cgcctccgcc accgaccacc accgttgaac cggtgacgac
    19501 aacgcacgtc accacgacga cgcagccgac gaccaccacc agaacaacaa caacgacgac
    19561 aacgacaacc accacaacaa cgaccacgac caccacgacg ccgacgacaa ccaccacgac
    19621 gacgacgacg ccgccgatga cgacgtcgta catcaccgtg ccgttcgtgc cggtgccgat
    19681 cccgattcag gtgccaaatc agggtccgca gagtcaggct ccccaatacg aggctccgca
    19741 gtacccgtac cagtaccagc agcctcagca gctgccctga gcaaccggtt cctcgacgtt
    19801 gcgcggatgg tggcaggcgg ccacatgagc cgccgcgtgt tcgagcaagg ctggctcgtc
    19861 ggtggtgcag atctcggtag cccaggggca tcgcgtgtgg aagctgcagc ccggcggcgg
    19921 gtcgatcggg ctgggaacat caccctgtag caccagccgc tcgcgtgcgg cgttgcgttt
    19981 cggatcggga atggggaccg ccgacagcaa ggcattcgtg tacgggtgaa tcggcctgcg
    20041 gtataggtcc tcggtctccg aattctccac gaccttgccg agatacatca ccgcgacccg
    20101 gtcggatacg tggcgaacca cgccgaggtc gtgtgccaca aacagatacg acagaccgaa
    20161 ctcgtcctga agttccttga gcaggttgat gatctgcgcc tgcacggaaa cgtcgagcgc
    20221 agaaaccggt tcgtcggcga cgatcatctt gggctgcagc gccagcgctc ttgcgatgcc
    20281 gatacgttga cgttgcccgc cggagaattc gtgcgggtag cggttgtagt gctcggggga
    20341 aaggccgacc cggtcgagca gatcctgaac ccgtcgcttg agctcggcgc ccttggccag
    20401 cccgtgcagc tccagcggat ctccgacgat ctggcccacg cgcttgcgcg ggttcagcga
    20461 cgcaaagggg tcctggaaga tcatctgcat gtcacggcgc agcgggcgca gatcgcgacg
    20521 cgaccggccc acgagttctt cgccgagaaa cttcaccgag cccgacgtcg gctgggtcag
    20581 ctgcaggatc gccctgcaca gggtcgattt gccgcaaccg gattcgccga ccaggccgag
    20641 cgtttcacct tcgcgcagcg acaggctgac cccgtcgacc gcgcgcaccc gggcgacctc
    20701 gcggtcgacc accaccccgg acttgatcgg gaagtgcttg acgaggtcgg tgacctcgag
    20761 cagcggatcg cccttgctca cgaaatcacc tgcgcccgac gcgatttctt ctcctccggg
    20821 tcgagccagc agcggtcgag atggttgccg cccacgcgcg gctcgagcgg gggcagctcg
    20881 ctgcatttgt cgaactcgtg cgggcaccgg ggggcgaacc ggcagccttt cggtggtgcc
    20941 agcagcgacg gcggctgtcc ggggatctgc gcaaggcgag cgccgcgcgt ttggtcgagc
    21001 cgcatcagcg acccgagcag accccaggtg tagggatgct gcgggtcgta gaacgtctcg
    21061 tcgagtgtgg cgttctccac gatctggccc gcgtacatga ccagcacccg gtctgcgatc
    21121 tcggcgacca ccccgaggtc gtgggtgatc aggatgatgg caaggtcgcg gtcgcggttg
    21181 agttggtcga gcaggcgcag gatctgggcc tgcaccgtga cgtcaagggc ggtggtgggc
    21241 tcgtcggcga tcaggacgtc cggttcgagc gcgagcgcca tcgcgatcat cacgcgctgg
    21301 cgcatgccgc cggagaactc gtgcggataa tgccgcacgc ggcgttcggg attcgggatg
    21361 cccaccgaac gaagcagttc gacggcccgg tcggccgcct cgcgtttcga caagtcacga
    21421 tgcgcccgga tcatctcggt gatctgatcg ccgacgcgat agacggggtt cagcgaggac
    21481 atcgggtctt ggaacaccat ggcgatctgc tcgccgcgga cgtcgcgcag ctcgtcgtcc
    21541 tcgagcccgt tgagctcacg gccgcggtag gtgacggtcc ccgtgatcct ggcgttggag
    21601 gcgcgggtga gcccggtaat tgtttgcgcc gtaacgcttt tacccgaacc ggactcgccg
    21661 acgatggcca gcacctcgcc ggcccgcagc tcgaacgaga cgccaccgac cgcctgcacc
    21721 acaccgtcct cggtggcgaa gctgacattg aggtcgtcga ccttgaggat ggtgctcacg
    21781 ccggtgccgc ctcaccaagc cggatccgcg gatccagcac tgcatacgcg aagtcgacga
    21841 tcgtgttgaa cagcacgatg aagaacgcgc cgaacagtgt cacaccgatc agcggcggca
    21901 ggtcgaggct gcggatcgcc tcccccgcat acagtccaac cccgttgagg ttgtacaccg
    21961 tttcggtgag gatcgcgccg cccccgacca cctgcccgaa gtcgagaccg aacaacgtca
    22021 cgatcggaat catcgagttg cgaagcacgt ggcggatacg cacctgacgc tcggacagcc
    22081 ccttagcccg cgcggttcgc acgtagtctt cgttcatcgc gtcgagcatg ttggagcgca
    22141 acattcggct gtagaacccg acgaagagca cggcgagcgt gaaccacggc aagatcaggt
    22201 gggacgccca acccactgga ttctccgtca gcggcacgta actgcctgtc gggaagagct
    22261 ggatcttgaa cgtgaggaag tacagcagga tcgcggccag ccagaacacc ggcatcgaaa
    22321 tacccaccag cgaaaggatt gtcagcgcgc ggtcggtgaa cttcccggcg tgaatggcac
    22381 tcatgtaccc gaacagcacc gccagtgcca tccagatcac cgcggccccg atgcacaacg
    22441 agaacgtcgc gggcaccccg tcccagatct gctgcacgac gttgcggtcc ttcgcatacg
    22501 acatgagttg gccggtgaag atctgtttca tcatcgtcag gtactgcacc ggcagcggct
    22561 gatccagccc gagatccgca ctgaccctgg caatcagcgc aggatcggcg ttcttgcccg
    22621 cgatgcgcgc cgcggggtcg gagttcggga tgacgttgaa gatcaggaac acgatcaccg
    22681 agatcgcgaa cagcactgcg atcatgccga gaagacgcct cacgatgaat ctggccatca
    22741 gtgctccagc cggatcttcg cgcgtggatc gagtgcgtcg cgcaggccgt caccgaagac
    22801 gttcaaggac agcacagtcg cgatgatcat caggcccggc acgatcgtca gatgtggtgc
    22861 tgtgtagatc atttcgtagc cgtcggcgat catcgtgccc cacgacgcgt tgggcgggcg
    22921 cacacctgcc ccgaggaacg acagcgccga ttcgagcagc atattgttgg cgatgttcag
    22981 cgtgaagaac acgatgatcg tggagatgat gttcggcagc agttcggaga acatgatccg
    23041 caacggcccc ttgccctgcg cgacggcggc ctcgatgaac tccttctccc gcagcgccag
    23101 gatttcgccc ctgatcggcc gtgccatgta cggcacgtaa acgaagccga tgatcatgat
    23161 cggaatccag atcgaatcgc ccgcgatcgc gaggggtccg atcttgaggc cgccgatggc
    23221 cagcgcggta cccaacgcga tgccgaggag gagcaccggg aacgcccaga tcacgtccat
    23281 gatccgcgac agcaccgagt cgatccagcc gcggtagtaa ccggcgagca gggcgacgat
    23341 caccgagagc accgtggtga ccgcggcggc cgccaggccg atgtagatcg acgtgcgccc
    23401 gccgtacatc agccgcacca tcacgtcgcg gccgttctgg tccgcgccga gcaggtactg
    23461 cccgtgcagg cccggtccga tcggggtgcc gtcaggcgcc accacattgg tcggttgccc
    23521 gtcgatcacc accttctcgg tgatgtggtt ttcattcgga ccggtgtgtg cgacatgttc
    23581 ggcccacagt ggcgctgcca ggcagaacag cacgatgagc acgaacacgg cgccgcacac
    23641 cagcgcaacc ttgttgcgcc gcagccggag ccacgccaga taccagggat tgcggccttg
    23701 gatctgcgat gcgggcacac cggtgggaag tggcggttct cctgggacct ccagttcgcc
    23761 aacggggacg accatgagtg gctacttgat cgcgaaggac gagtaatcct ggttgaacag
    23821 cagatgatga taggacttgt cgaagtccat gcgttcggac aggaacgtgg tgaatcgctc
    23881 gttgccgtag ggcgcccaga ccgcctgctc catgtacgcc ttgtccaggt ccgcgtactg
    23941 ctgctcgacc ccgccgtcgc cgatcagctt cgtcagcagc tcgtcctgct tcgcgttgtt
    24001 ggcggggatg ttgacccgcg accaattgtt gctattggtc ggcaggatgc tgtcaccgtg
    24061 cagcagcggg cggaagaagt catccggatg cgggaagtcc tggaaccagt ccccgaaccc
    24121 cgtgtccagg tcgggggttg actcgttgcc gatcgtggtg aagtagacgt cgcctgcgat
    24181 caccttgagc gtggcgttga agccgagctg ggtgagcagg tcgtggtagt actcgccgat
    24241 ccgcttgcgg tccggctcgt cattcgtcca caccgtgatg ttgcggtccg ccgggttggc
    24301 ttctgcgatc agctgtttcg ccttgttcat gtcgggtccc gggaacagct tgtactcgga
    24361 gtagccgggc atcccgggcg ggaggacctg ctgcgtggga tgcatgcggc cgccgaagat
    24421 ccggttcagg gcctcggggt cgacggcgta gttgatcgcc tggcgcacct tgaggtcgtt
    24481 gaacggcggc ttttgggtgt tcatccagaa gaagtaggtg ttgatcgaat cttccatccg
    24541 gaagcggttg ccgtagcggg tcttcacctc ttgcagacgg tcggatgccg gcgggtccac
    24601 catgaaatcg accttgttct gctcgatgtc ggtgacctgc gcgctgttgt tcttgttctc
    24661 tgtgacggtg atcttgtcca cgctggcgtc gaccacctcg tcggccccgg cgtccttgac
    24721 cgtcttgaag ttcgggttgc gctccatcgt cagggtgcgc ggcgcctcca ccttgctgat
    24781 catgaacgga ccgctcgacg gtggcgggtt gttggtggcg tccttgtcca gcggggtgct
    24841 cggcggcacg ggcgcggcga acatgagccc gagcacgttc tcgaaggtgc cgttggcctc
    24901 ggccagttcg atggtgatgt cgccggtgtt gtcgtcggtc ttgatgccgc tgatcgtgtt
    24961 cgccttgccg tcggcatagt ccttggcgcc gacgatgcca ttgaagaaca ccgagccgcc
    25021 ggagtcggtc ttgaacagcc gctggatgcc gtaggcgaaa tccgatgcct tgatgggcgt
    25081 tccgtcggag tacttcatgt tctgccggag cttgagcttg tacgtcttgc cgtcgggcga
    25141 gatgtcgggc atcttctcgg ccagcgccgg taccacctgg gtgccttcct tgcccttggc
    25201 gtgcttgtac gtcagcaacg gggtgtagac gttccagagc acctcccagc cctcgagggt
    25261 gtaggagagc tgcgggtcga tgtagtcggg gaacgaggtc agcgcgacgt tgatgtcgcc
    25321 gccgcctccg ccgccaccgc cgcctccgcc cttgtcactg ccgcacgccg cgacaccgaa
    25381 cgctgccagt ggggtgatgc agacgatggc gacaaccttg cgcagtgtgt tcatctcgct
    25441 ccctgtggct cgcgtgtggg ttgactcccc taaagctgta cgccttccgg tggggtcgag
    25501 ttgtcatctt tgaggaaaaa attggcacgg caactcgatc gttttcgcac attcgttgtg
    25561 ttcgtttgcc ctggatgttt gccgggtcaa tcaataacta ccaccgtcaa ttttgctcga
    25621 ttccaatacg gacgaagtta actgtggcat tatttcggca gggctacatc cgtgcgccgg
    25681 cggccaccgc ttgtcaagaa tcggctagtc gcggggcctc atagttagga aacaatatgc
    25741 tgtcatctct cctggctcgt cgtcttgcgg ctgttgccgg aggtgtagcg atcgccgcca
    25801 tggtcggtgt caccgcctcg tgcgccaagg aagaaaagaa agcgcccgag accacaacaa
    25861 cgaccaccac caccaccacg acaagtgcct cgccggcgcc gcctccgtcg ccaacagaga
    25921 agtcgatcaa cccgaccggc ggcaacctgt tcaccccacc ggtgtacgcg ccgccggcgc
    25981 cgacagctat ccccggcgac cactgagttg gtccgccaac gcgggatcgt cggctggcgc
    26041 gtgtcggcgg tcctcgcggc gggggtactc gtgctcgcca cgggcgtgcg ggctgcgtcc
    26101 gtgtccgacg accggccggt catcggcggt ccgctggcgt tattactagc cagctcaacc
    26161 gatctcggcc catcccgcga tggcggtgcg caactcactg tcacgttggc tgactcggct
    26221 cggccggcga cgttaatcgc atgggccagc gcacacgaca tgtgggtgcg gtggcgaccg
    26281 ggtgacacgt gggcagtcgt cgagggtgcc gcgcaggacg tggcaacggc gttcgcggtg
    26341 ccagtgaacg actaccgcgg tcgcaaaggg caggtgttct acgcgtcgcc gcagcagccg
    26401 tcgattcccg agactcttcg cgccagcgtc accggcatcg ggcgaatcct cgggtacacc
    26461 ccgcaccgcg aggcgcgtcc cggattcctg ccgctcgacg tgccgagcca ggggctcacc
    26521 ccgaaagcgt tgctggaggc atacaacgcc aatccgctgg ccgccgcggg cttcaccggc
    26581 aagggcgcga cgatcgtgtt cttctcattc agcgggtacg accagaagga cctcgacgcg
    26641 ttcgccgact tgtccgggct gccgagattc actccggtcc tggtcggcgg tcagcccggt
    26701 ccatctggtg ccgagacggc gatggacctc gaagtcgcgc atgcgattgc gcccgacgcg
    26761 cagaaagtcg tcgtcaacgc ccggccgacc gtcgaagggg acggcgccta cgaaaagatc
    26821 ggacagctgt tcgagtcggt ggaccggcag ttcccgggcg cggtgtggag tctgtcgatc
    26881 gggtggggat gcgatgcgct gatcagcgcc gccgacctgg cccctatcga ggccgccttg
    26941 gccgccgcgc agaaacgcgg aacctcagcg ttcgacgcga gcggggacac ggccggtctg
    27001 gagtgcaagg gtggcgacga atggtcgtcg aaccccggcc cggatgatgt tggcctggac
    27061 gctatttcgt cgttgccggc gatgacatcc gtcggcggta ccaccctgtc gactgatgac
    27121 cagggacggt ggctcgccga gcgcgcgtgg gtggactcgc cgttgtcgca gggcaccagc
    27181 ggcggggtgt cagtgctcta cgaccgcccc gcgtggcagg aacggctgtc ggtggagcgc
    27241 gacgttgatc ggcgccggct ggcccccgat gtcgccgcgg tcgcggatcc gtttaccggg
    27301 gtcaggattg tcttcgacca gcaggaattg gtcggtgcgg gcacctctca ggctgcgccg
    27361 atctgggccg gactggccgt gttgatgaac cagtatctgc tcgcccatgg cgggcggcca
    27421 ctagggcaca tcaacccgtc gctgtaccgg gtcgcggcgg gcgcgaacct gcccgcgttc
    27481 cgcgacgtgg gccttggcgg gaacgccgtc gatatctcgc agcccgggta cgacctggtg
    27541 accggcctcg gcagccccaa cgtcgactac cttgtccgcg acctgctgga catccagagg
    27601 ggtgtcgctc cccgatgacc accggcgtgc cggtgatgcg gtgtccggta tgcaacgcgg
    27661 acgtgcccgc ggccgcgttc tgcggagcgt gcggtgcaca cctgtcgccg cgccgcgggg
    27721 atggccgggg catgctgcgg ccggtcgcgt atggcgcggc ccccggcgag cacgtgctgc
    27781 ggctgtccgt ggcgagcacg ttgtttccgc atctgccaca ccggtcgcgc gcgccgtttc
    27841 gggttgcgct ggccgtcttg tttctgacgc tggtcgtcct cgcgttcctc ggatggcagg
    27901 caccgttgat cgcggtcggt gcgctcgggt tcccgatggt attcgtgctg tatctgcacg
    27961 agaccgacat cgatgacgat ctgccactga gctcccttgt gctgactgca gtgctggcga
    28021 tcggacttgg catcggctgg gcgctactga ctggcgtgat cgtcgcgcac tcctacgatg
    28081 tcacgctgag cgacgcaagc tccgaagagc attcaatacc cgttgagggg atcgccatcc
    28141 cggcgggtac tgcggtcctc ttggtgcttc ccgcggtagt gatgcggctg ctgcggccag
    28201 gaacccgaga gtcgttggac gggttcgtga tcggcgcatt gagtgcgatc gcctttaacg
    28261 cggcggcgac gctgacgcgg ctgtcccccc agtttgcgac ggggcccact gcgcacgacc
    28321 gaccggccag ctcactgctc gccgaggcgg gcatccaagg ggtggcgatg ccgttgacgg
    28381 ccgcagcgtt gggtggcctg gctggcgtgg cgctctggtt tgcccggcct ggcgataagg
    28441 accgccgtcc ggcgattgtg ctgctggttg cgagtttcct tgtggtgctt ggtatttgcg
    28501 tcggactcgg attgctgggc gcgacctcgt tcgccaatgg cctgcacttt ggcttgagtc
    28561 tgctcgccgc cgttattgca ttgttggcct tgaggaccgg ggtgcaggcg gcgctactgc
    28621 acgaagcgca tgacgagatg aacccgcagg cgcaggtgtt gtgcccgcac tgcgaccacg
    28681 tcgtaccgga taccgccttc tgccccaact gcggggtcgc gacccgtgcc gcatcgcgga
    28741 cttcgcgtgc cgcccgccgc accacacaag gggttacttc tgcccgaccg gggtacgcgg
    28801 tcccgccggg gtcgtatgcg gcggtgccgg tgcagcacac cacccacacc aaactgctga
    28861 cggcgttggg cgcgggtgca gcggtcgcgg tcgcagcggc tgtcacggct tcggtcctgg
    28921 ccaccccggt tgctgcgcgg tatgtctgcc cacctgattg cggggagcct ccaattggca
    28981 agcccgtcgc tgcaaacccg tggttcacgt ccggtgacgg caagttcgcc gtgcagtatc
    29041 cgcgggccgg tacggcgtac gaggtgaaac tggaatccga aggtgtggaa gtgaacttca
    29101 cagccggcga caccggcacg atggagttgt tcgggatgcc cgcgagcaat cgcaccccaa
    29161 agcagatcgc cgaggccttg atcaacgagc actatcccga cgccacaaca gattacgaaa
    29221 ttcccaacgc catggtcgga taccaaccgg ggtacggggt ggtcaaagac gaatatccgc
    29281 aggacgcgtc gggaacgttc acgcgccttc gactcctggt catggtggcg gtcaagaacg
    29341 actacgcctt ggtcgccgcc gcgatcgggc cgtaccacga gttcagtccg gattttggaa
    29401 gcggtcatcc ttctggcgtc aacctgcagc tggcactcga tatgggcaaa tacgtgaaca
    29461 gctttacctg gcgcgacgat tcgccccgct gagccttacg tggtgtcgga tagacccatg
    29521 aaacgtaaac atgtcctcgt ggcaggcgcc gcgattgttc tcgccggtgt ttcggcaggc
    29581 tcggccgccg cgaccccaag gagggcaccg tcgagatcca gcaggcaccc ggcgcctgtc
    29641 cgtagcggag atcagccgta cggttgcggc tcgtggaacc gattgatgat cgggatgcgc
    29701 tcgatgatcc ggtcgcgcag ccgcggctgc tgcgggacgg gcggcacata gttcggcgcg
    29761 ggggccggag gcacgtaggc cggcgcgggc ggtggtgcca caggaggttc ggagttaacg
    29821 ggcgcctcag gcggcgggac gaccgggatc gcgtctggaa cgggcgccgc agcctccacg
    29881 atcgtttgcg ctacgggcgg tgcgggctcc agcggtggcg ccgcaggcgg tgacggcggt
    29941 gcaaccggaa tgcgttgcgg tggagcctgt tcgacc
//
LOCUS       DASRJA010000148         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2226433,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000148 DASRJA010000000
VERSION     DASRJA010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2226433"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFC01_06975"
     CDS             complement(<1..205)
                     /locus_tag="VFC01_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion-associated serine protease"
                     /protein_id="HZN79417.1"
                     /translation="MTRIARLCIAGVLATFPLWPAPPALGVTPPAVDDSMLPKPGPPA
                     PPQSTKQLEGCVAAPSASVGTHDP"
     gene            complement(202..1524)
                     /gene="eccD"
                     /locus_tag="VFC01_06980"
     CDS             complement(202..1524)
                     /gene="eccD"
                     /locus_tag="VFC01_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion integral membrane protein
                     EccD"
                     /protein_id="HZN79418.1"
                     /translation="MPDALCWLSVAHGPHTVDLALPMDAPVGTLLPSIVDLVHQGTVA
                     ADEARQWHLSRVGDQRLDEAMSLEDNAIRDGDLLLLTTKAAPAAVWSQDDPWHAAVDT
                     MDARRPPPRVGSAVVCLCTAVIGASALVWSGAVTHAIGNVITAGVIAAVAAVAVVAMW
                     RAHADPVLSVASSVITIVFAAAAGFLAVPAGPSMASSLLATAVACSTSVLLLRVTQCG
                     AICLTALAALTALTSAATACGVIWSLPIFTTGAALSVLSLGTLGLAARLSLTLAGLTP
                     AVPSPDDDPAPKAARVATAHDTLSGLVIGSAAAAALGGVLVISGHSGSWPRATVFSAV
                     VGLVMLLRVRTHIDLRRRIALMVAGHIAVAAGVVLVVISAPGQANWVCVLATAIGLAA
                     LGGAVGVTLSPLARRAVDLVEYLALAAVVPLACWVGGVYGLVRGLSLP"
     gene            1603..>2671
                     /locus_tag="VFC01_06985"
     CDS             1603..>2671
                     /locus_tag="VFC01_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79419.1"
                     /translation="MDGTNRTIATSFSSMDFSREPRLPAPSVAEGDIALDPPPEVPKA
                     LPANPLARLLPVAVLVATGGMMALYFTSGAAATTRGPMFMFFPVMMLASVVGSLAYGS
                     RGANHTAQLNEDRRDYLGYLDALEETIAKTAVDQHRALHWIHPDPNGLWTLVGGRRMW
                     ERRMDEPDFCHIRVGLGDQSLCATLVAPEPGLDPVTSSALQRLIRRRSVVADLPVVVP
                     LQELSAVIIEGDPAAARALLRAIVCQLAVLHSPDDVRIAAVVGPISGADWDWLKWLPH
                     HQHPRLVDVLGPARMTYRSLGDAITDCRRDDGPSPHVVIIIDDGSPPAIEQPFNEAPR
                     LELRLDGDMRPDGMTSSQAARC"
BASE COUNT          487 a          896 c          872 g          416 t
ORIGIN      
        1 ctgggtcatg cgtgccgacg ctggcggaag gcgctgcgac acaaccctcc agctgcttcg
       61 tggattgcgg cggcgccggt gggccgggtt ttggcaacat cgaatcgtcg accgccggag
      121 gtgtgacccc gagggcaggc ggcgcaggcc agagcgggaa cgtggcgaga acaccagcga
      181 tgcacagcct tgcaattcgc gtcatggcag gctcagcccg cgcaccaggc cgtagacgcc
      241 gcccacccag cacgccagcg gcaccactgc cgccagcgcg aggtactcca ctaggtcgac
      301 tgcgcgccgt gcgagcggac tgagcgtgac gccgaccgct ccaccgaggg cggcaaggcc
      361 gatagccgtc gcgagcacgc aaacccagtt ggcctgtcct ggtgccgaaa tcacgacgag
      421 cacgacgccg gcagcgactg cgatgtggcc agcgaccatg agcgcgatgc ggcgacgcag
      481 atcgatatgt gtgcggacgc gcagcagcat caccagtcca actacggcgg agaagacggt
      541 tgcccgaggc caggagccgc tatgacccga tatgacaagc actccaccga gcgccgcagc
      601 agccgccgac cctatgacga ggccgctcaa ggtgtcgtgg gcggtggcta cgcgggccgc
      661 tttcggggcc gggtcatcgt ccgggctggg cacggcgggc gtcagaccag ccagcgtgag
      721 ggataacctc gctgccagac caagcgtgcc gagcgacagc acggatagcg ccgcacccgt
      781 cgtaaagatg ggcaacgacc agatcacacc gcaggcggta gctgcgctcg tcaacgcggt
      841 caacgcggcc aaggccgtca aacagattgc gccgcactgg gtgacgcgaa gcagcaagac
      901 tgacgtcgag cacgcgaccg cggtggcgag cagcgaactg gccatcgacg gaccagcagg
      961 cacggcaaga aacccggctg cggcggcgaa cacgatcgtg attacgctcg aggcgacgga
     1021 gagaaccgga tcggcgtggg cgcgccacat ggcgaccact gcgaccgcgg ccacagccgc
     1081 gatgacacct gcagtgatga cattgccgat cgcgtgtgtg acagcgccgg accacaccaa
     1141 agccgatgca ccaatcacgg cagtgcacaa gcagaccact gccgagccga cccgcggagg
     1201 cggccggcgg gcatccatgg tgtcgaccgc cgcgtgccat ggatcgtctt ggctccagac
     1261 ggccgcaggg gccgctttcg tcgtgagcaa cagcaagtcg ccgtcgcgaa tggcattgtc
     1321 ttccaggctc attgcctcgt cgagccgctg atcgcctacc cgagataaat gccactgtcg
     1381 agcctcgtct gcggcgactg taccttgatg cacgaggtcg acgatcgacg gcagtagcgt
     1441 tcccacgggc gcatccatcg gtagtgcgag gtccacggtg tgtggtccat gcgcaaccga
     1501 tagccagcac aatgcatcag gcatcgcatc accccccgtt gacgcctgcg ccttcaccgt
     1561 aactccgtct gcgcgtatgg atgcgcggca gtttcgcgac ctgtggatgg aaccaaccgc
     1621 acaatcgcaa cgtccttttc gtcgatggac ttctcccgcg aaccgcgact tcccgcaccc
     1681 tcggtggccg agggtgacat cgcgcttgat ccaccgcctg aggtcccgaa ggccttgccg
     1741 gccaatccgc ttgcccgtct gctgccggta gccgtgctcg tcgccaccgg cggcatgatg
     1801 gcgctgtact tcacctcggg cgccgcggca acgacccgtg gccccatgtt catgttcttt
     1861 cccgtcatga tgttggcgtc ggtcgtcggc tcactcgcat acggatcccg gggtgcgaac
     1921 cacaccgcgc agctgaacga ggaccggcgc gactacctcg gctacctcga cgccctggaa
     1981 gagaccatcg cgaagaccgc tgtcgaccag catcgcgcgc tgcactggat tcatcccgac
     2041 ccgaacgggt tgtggacgct ggtcggtggc cggcggatgt gggagcgccg aatggacgag
     2101 ccggacttct gccacatacg tgtcggcctt ggtgatcaat cgctctgcgc gacgctcgtg
     2161 gcacccgagc ctggtttgga tcccgtgacg tcgtcagccc tgcagcggct gatccgacgc
     2221 agatcggtgg tcgccgactt gccggttgtg gtgccactac aggaattgtc cgcggtcatc
     2281 atcgaaggag atccggccgc cgcgcgggca ctgctgcgtg ccatcgtctg tcagctggct
     2341 gtcctccaca gccctgatga tgtccgtatc gcggctgtgg tcggtccaat atcgggggcc
     2401 gattgggact ggctcaaatg gcttccccac caccagcatc cgcggttggt ggacgtgctc
     2461 gggccggcca ggatgacgta ccgcagcctc ggcgacgcga tcactgactg caggcgtgac
     2521 gacggcccgt cgccgcacgt ggtgatcatc atcgatgacg gctcgccacc tgcgatcgaa
     2581 caacccttca acgaagcgcc acgcctggag ctgcgcctcg atggcgacat gcgtccggac
     2641 ggcatgacgt cgtctcaagc ggcgaggtgc g
//
LOCUS       DASRJA010000149        30678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1110220,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000149 DASRJA010000000
VERSION     DASRJA010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 30678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30678
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110220"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFC01_06990"
     CDS             <1..418
                     /locus_tag="VFC01_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN79420.1"
                     /translation="CQVFSKRGLSVDLGGSWLLPKLVGLQQAKRLVLLAETIDADEAR
                     SLGLVTWVKSSGEIDAFVSDLAGRLAAGPPVALAQSKGLLNDGANATLREALANEARA
                     QPGNFATADSIEAYAAFAEKREPAFTGRWAVPPRSE"
     gene            424..1569
                     /locus_tag="VFC01_06995"
     CDS             424..1569
                     /locus_tag="VFC01_06995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZN79421.1"
                     /translation="MRETVIVEAVRTPVGKRNGGLSDMHAADLSAIVLDALAERAGID
                     PDIVDDVVWGCVSQVGDQSSNIGRYSVLAAGWPESIPGTTINRACGSSQQALDFAVQA
                     VMSGQQDVVVAGGVEVMSRVPLGSARATGMPYGPKVLARYGDFSFNQGISAELIAQKW
                     GFSRTRLDEYSARSHELAAKAQDDGAFETQIMPVFTGGEPVVADEGIRRGTTVEKLAG
                     LKPAFKEDGVIHAGNSSQISDGAAALLVTTAENAITMGLTPIVRYRAGAVTGADPVLM
                     LTGPIPATEKVLHKAGVGLDEVGVFEVNEAFAPVPLAWLAETGADEDKLNPLGGAIAL
                     GHPLGASGAVLMTRMINHMRDNGIRYGLQTMCEGGGTANATLVELIS"
     gene            1583..2731
                     /locus_tag="VFC01_07000"
     CDS             1583..2731
                     /locus_tag="VFC01_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN79422.1"
                     /translation="MRRDLFTEDHEAFRELARDFVEKEVVPHYPEWEKGGRMPRDVFK
                     QMGALGMLGMAIPEEYGGGGAPDYRFNVVLQEEAARALVTLSTVRTQLEVILPYFLHY
                     ANEEQRKRWFPGLAAGTLLTAIAMTEPGTGSDLAGMRTTAVRDGDDWILNGAKTFITG
                     GMQADLVIVVARTATDPDNRRKGLTLFVVEDGMEGFTRGRELEKMGCKVQDTAELSFV
                     DVRVPAANVLGEEGEAFGYLGHNLPQERLTVAVGSVSQARSAIAAAIDYTKNRKAFGT
                     PVASFQNTKFELAACSTEVEAAQAMVDRAVALHVESELSAADAARVKLFCTEMQQRVV
                     DRCLQLFGGYGYMMEYPIARLYTDARVARIYAGTSEVMKVIIAKSLGL"
     gene            complement(2744..3661)
                     /locus_tag="VFC01_07005"
     CDS             complement(2744..3661)
                     /locus_tag="VFC01_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN79423.1"
                     /translation="MEQNLPFDVDALLVFGKVVESRSLSKAAALLGMPKSTVSRKLTK
                     LEADLGIKLLRKNTHQLTVTDLGEKVYDHAVNILAEANGVRALVEGSRQEPQGELRVA
                     IPVFVGVDYASRVGATFLQRYPHSRLDIRLVDNMVDPIKDGFDVVFGTGPLQDSTLIA
                     RKVFDLELFLCASPHFVQQLTEPITDPVQLNGLPFIEFGFRGPHKLTVVNHKRRYELA
                     PQIRARANNFQVCKQYILQGLGIGEMPTEIICTDELRDGSIVPVLPQWKPDPLEVHMI
                     YPFELSFSTLISAFYEAACDIIVENIARA"
     gene            3754..5028
                     /locus_tag="VFC01_07010"
     CDS             3754..5028
                     /locus_tag="VFC01_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZN79424.1"
                     /translation="MSHNSLVTKPASGHWSDAYPELGRGPVSLEDCVSPEFYEKEREH
                     VFEKTWLYVGRVERVPKSGSYFTRELRFLNTSVIIVRGKDGVIRAFHNICPHRGNKML
                     WEDDPFQEVSGRAPLLYCRFHGWRYKLDGSLHSATRKDLLLDFDADSCRVPAIQCEVW
                     EGFIFINLNPHNTEPLRSFLGELAYGIEGYPFEGPHQVYRFNTELQCNWKIFVDSFAE
                     SYHGPYLHASSFGALTAEARDAFDQPNPFTDALAYQLNGPHRMFSFSGEPSQKTPYSK
                     PIECVMEASAAGPWNKRTDRGPMPPGLNPTRSEKYGFDSYQFFPNFVLIFGASGFSTH
                     VHWPTGPHSHIFEVEMFYQPPKSHKERLGQELTVTFLNDIILEDASPSEGLQAMLNSG
                     ALTHFTMNDEEILLRHLHKTVADYVQAGEAAR"
     gene            5025..5348
                     /locus_tag="VFC01_07015"
     CDS             5025..5348
                     /locus_tag="VFC01_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009955127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79425.1"
                     /translation="MSALLPPEFSQLDHLVGEWAIEDGHARYVKRVNSSMEQIQAFYD
                     EVFPVAEEAVAYIDKFDYSEPLPDDVANLRNLLYSLITVSLAVELWKQPRVKHSANTI
                     LTRVS"
     gene            5355..6185
                     /locus_tag="VFC01_07020"
     CDS             5355..6185
                     /locus_tag="VFC01_07020"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZN79426.1"
                     /translation="MALTSAQFDIVDRAPRIGSEIRTDLETLLSGREAANIRETLEQR
                     GVVFFRGLDISDEQQVAIAKTLGNIVRNEGDSGIYKISLDKNVNQRAEYLKGSMFWHF
                     DGSLQPYPNLATLLRAIKLSETGGDTEFCNTYAAYDDLPQADKDLIADLRVVHSAERS
                     QYYVRPEMSYDEITFWQKSPTKACPMVWTHQSGRKSLLLGATADYVIGLPVEESRALL
                     ARLRDWATQPQYVYRHEWQPGDLLVWDNTGTMHRVLEYPVDSGRLMHRTILAGEEPLQ
                     "
     gene            6182..7051
                     /locus_tag="VFC01_07025"
     CDS             6182..7051
                     /locus_tag="VFC01_07025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZN79427.1"
                     /translation="MKLGIATPVVTNVGGAALTWEKDATIEDIGRVAETADQLGYHHM
                     TCSEHIGLPSSETARRGARYWDPLATFGFASARTKQIRFATMTLVLGYHHPLAIVKRY
                     GTLDHVSNGRVILGLGVGSLKEEFDLLGAPFDDRGARGDDALRALRAALPSNKPTYEG
                     EFYSFGGLTVDPCALQPHMPFWIGGRTKRSLRRAVTLADGWCPFYVSIDTAAEWLKTF
                     DVPPGFEVVMPADQPLDPMGEPGATKETLETMAAGGTTTLSARFVHHSLEHYLEQIHA
                     LAEMHDPAGGRCT"
     gene            7048..7656
                     /locus_tag="VFC01_07030"
     CDS             7048..7656
                     /locus_tag="VFC01_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN79428.1"
                     /translation="MTGPLLSPLSADEWTDDAYAAVGALFGVPGEAIPRAGSGEARDP
                     LNFDILGVLARHPDLARVFLSYNAFLLQRGELPLRLRELAILRLAHVRRSAFFWGEHV
                     RVATAGGLSEDDIAGLARGNVEFDGTDRLVLEATDEMLADGRARPAMWKRLVEELGTH
                     QAMELIFAVGTYGMLAMACDTWQLVPPPGSAKLPAETARDDD"
     gene            7828..8445
                     /locus_tag="VFC01_07035"
     CDS             7828..8445
                     /locus_tag="VFC01_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79429.1"
                     /translation="MTSASGSRPYATLLAKGEDRRQRILSVAERLLARNGWRNTSLAL
                     IAKEAGVTPAGLLHHFESKEQLLNAVLDARDADDDAHADYRRGDLITELSRVPERFER
                     APELIGTFTVLLVENIAPDAPLHDRIHKRYRDAIDIISDIITRGQRDGLYRTDFDAAS
                     KAVEILAFITGMETLWLLDPSIPLTEVFKGYAESLGREIEPRSST"
     gene            8442..8942
                     /locus_tag="VFC01_07040"
     CDS             8442..8942
                     /locus_tag="VFC01_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79430.1"
                     /translation="MRYRLDVVAPTVLDAVRFAGGWVYDRVMAGWDVTVLIGGDDDVR
                     PLEILGTEVRDLESVLESWEDRPHPQTVAVAGDLFAVDARVRGHVLNALDQGATEVTL
                     WGDSLPAELDDSVDSVQHRLSAAARAFKAQALAAANGPAGDVGQTETFRCGRMASVAA
                     DLIPAS"
     gene            complement(8953..9918)
                     /locus_tag="VFC01_07045"
     CDS             complement(8953..9918)
                     /locus_tag="VFC01_07045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HZN79431.1"
                     /translation="MRAAVCPAYGPPDVVRIEEQPSPAIESGHVRVKVGAAAVNFPDV
                     LLIANEYQISVPPPFVPGSEFAGAIVETGQGAVGFAVGDRVTGTGLFGAFAEEVAVPA
                     AGLSRIPDGVDDRTAAAFGVAYRTAYHTLRSMARVQEGDELVVLGAGGGVGLAAVQLG
                     VQLGASVTAVASSAEKLDVAAGYGAKHVINHKAADVRAALKEAVPGGADVVVDPVGGE
                     LSEPALRSLGRGGRFVTVGFASGVIPRIPLNLVLVKGIHVLGFQFQDVPPDEFARNDN
                     ELRELLVSGRVLPHVCAVYPLAETAAALRHVADGRAIGKVLIDLT"
     gene            complement(9915..10898)
                     /locus_tag="VFC01_07050"
     CDS             complement(9915..10898)
                     /locus_tag="VFC01_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZN79432.1"
                     /translation="MDGEALPGKGEPLDAQFLSGGTQNVIYKVNRGEHECVLRMPPPD
                     APPDRDKGILREWRIIEALDGTDVPHTKAGGVCSDAAVLGRPFYLMGFVDGWSPMDTH
                     ARWPEPFDSDMEARAGLSYQLAEGIALLSKVDWKGKGLQDLGRPDGFHERQVDRWIAF
                     FERIKGREIDGLDVATEWLRAHRPIDFIPGLMHGDYQFANVMYRHGAPAQMAAIVDWE
                     MGTVGDPKLDLAWMVQSWPEDTDAPSAMSYVDMRGMPSRTQVVDHYAEVSGRQVDDLD
                     YYLVLAKWKLAIVLEQGFQRAGDNEKLLAYGPVITTLMRSAADLAESTDYQ"
     gene            complement(10931..12232)
                     /locus_tag="VFC01_07055"
     CDS             complement(10931..12232)
                     /locus_tag="VFC01_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN79433.1"
                     /translation="MAWDFETDPEYQKLLDWADEFVREECEPLDLAFPHLQFVPLDDS
                     RRKVIDPLKEEVRRKGLWATHLGPELGGQGYGQLKLALLNEILGRSQWAPIVFGCQAP
                     DTGNAEIIAHYGTDEQKEKYLRPLLDGELFSCYSMTEPHAGADPTLFTTRAVRDGDDW
                     VINGWKFFSSNAKTASFLIVMVVTKPDVSAYQGMSMFLVPANTPGVNIVRNVGLHGEP
                     DDEGSHALIHYDNVRVPAEALLGGEGQAFVIAQTRLGGGRIHHAMRTIGLSHKALDMM
                     CERALSRQTQGSLLSDKQFVQGYIADSYAQLLQFRLMVLYTAWEIDKYNDYKKVRKDI
                     AAVKVVMPTVLHDIAWRAMQVHGALGVTNEMPFMGMVTGAAVMGLADGPTEVHKTTVA
                     KQVLRGYQATDDTWPTEWTPRKREAARAKYADYLENEVGNL"
     gene            complement(12245..13255)
                     /locus_tag="VFC01_07060"
     CDS             complement(12245..13255)
                     /locus_tag="VFC01_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN79434.1"
                     /translation="MKVSVVTAPGATQVIDAPKPTVGPADVLVKIRACGICGTDALFI
                     SMGGIPGHDDGMPIGHEPAGEVAEVGSDVTGIAVGDHVVINPMAKAGEIIGNGATAGA
                     PSEYLLIENAVRGTSLEVVPDHIPFEVAALNEPMAVARHGVNQCRPKPGDKVMIFGAG
                     PIGLGATIAFKSLGVSHVVVADLIPARLEKALKVGADAVINSAADDVAKHLLELHGEG
                     ASMFGGKAGTDIYFDAAGVPAVIETAFAAAKTGARLGIVAVHKQPVSVDFLNLMSNEI
                     TIVGSMGYPNEIFEVTKDLIANWEKYAVIISHTIPFDDVNDALKTATTPGAADKVVVT
                     FN"
     gene            complement(13268..13921)
                     /locus_tag="VFC01_07065"
     CDS             complement(13268..13921)
                     /locus_tag="VFC01_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79435.1"
                     /translation="MARNGAVTAADGSQRRASFQRARSHETKRALVQAAMALWRTNGY
                     AKTTVADICRAAGVSRALFYFYFPAKEDVLFEVGLLSTRAAQKKVRSLLQTDYEVDAV
                     IAEALRSLERSMARNPRELIIETILEGYRHEHRILAGEPLEDADADMFGDLLAMAQRD
                     GKLAAHVDVRHLSHLAGMHVSEGVRHWAAGTYGDRSFADVVGRDISALIAGYNQLPA"
     gene            14107..14276
                     /locus_tag="VFC01_07070"
                     /pseudo
     CDS             14107..14276
                     /locus_tag="VFC01_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727029.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArgP/LysG family DNA-binding transcriptional
                     regulator"
     gene            complement(14342..15445)
                     /locus_tag="VFC01_07075"
     CDS             complement(14342..15445)
                     /locus_tag="VFC01_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZN79436.1"
                     /translation="MKSFVFAVVAAITAALAACSPPPEAPPLARPAESIQRDHALVVD
                     GEQIADPALWAHAKDEGQLTLYSGYTQDSEATVLDQFESDTGLKVKLIRLTPNRLYER
                     IVAEFGAGKLPADVIRISDAGFVSGLSKRGVFQPYHPPTATSLRDDVVFDGGNYYRTF
                     DPIYTFGYNTAVVDANEAPKTWKDLLSSRWHDKLGIAQAGAGGSALALTRFQREVLGD
                     GYLHEYSSGTRVFDSLGAELDALARGEIYAGTVVVSSVNIAINENAPVTFVVPTEGVT
                     AYDYYTGVAATAKHTAAARVFMNWNLSKRGQRVLHDIGEYSVRSDIPAPFVRGVQLPD
                     FDDPRVHRITPTQAIANAAEDQKTWNGIFGYNE"
     gene            complement(15442..17208)
                     /locus_tag="VFC01_07080"
     CDS             complement(15442..17208)
                     /locus_tag="VFC01_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZN79437.1"
                     /translation="MSAPAVNPPAATTGKPPTERAAVAPRRPHGKRWLPVLLQLAVLA
                     VLVLLPMFFIVMGAFTDKAERESLFDFGHFTLDNFAVIGSSTGRNALLNSAAVGIGSS
                     IVALTVGCLLAFLAARTDIPGRKAIYGIAITPLFLPALVGALAWALLAGPATGYLNLA
                     LNAVGLPHLINVYSFSGMVFVLGLYYAPYPFLMVHSALSLMNPDLEDAASLHGAKISQ
                     MLRKVTFPLVMPALLGSAILVFALTVENFPVAQVIGVPGGVDTLPTLIYRLMNASPAR
                     SNDAAAVAVVLTVLLIAVVAGQQKAMGGKEFTTVSGKGVRARRIPLRAWRLPAAAFAW
                     SYFLLAVLLPVGALIIAAMQTSPYVANLGQLTQPGALSLWGMGQTIQSSDFHQVVLNS
                     VVVGVATAAIGTAFCFALAYTRYRTKAPGRKLLEYVAMLPLAVPAIVLGLGLLWTWLA
                     LPVPVYGTLAVLVIAFVAVFLPQGYRGVSASIIQLDKDLEDSAVMLGARRHRAIASIT
                     APLLRVGLSSTFLLLLMLSMRELTAALFLFTSDTRLLSIAIFDAYDNGSLQSAAELSL
                     LYCAVIGVLAVLARRFGSKEIS"
     gene            complement(17205..18320)
                     /locus_tag="VFC01_07085"
     CDS             complement(17205..18320)
                     /locus_tag="VFC01_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN79438.1"
                     /translation="MKVTIENLTLPYQGNIVVRDLDLEIADGESLVLLGQSGCGKTST
                     MRCVAGLETPSVGRITIGDRVVHDSASGRSMPPHKRNVGMVFQSYAVWPHKTVFENVA
                     FSLRMQKVGKSELRQRVGEALDLVGLGELAERGASLLSGGQMQRVALARSLVMRPSVL
                     LLDEPLSNLDARLRERLRMELRELQLRLGLTTVYVTHDQAEAFALADRIALMQKGCIV
                     QIGTPQQIYTNPVCASVAEFLGIGNIFRCTKGTGPGSVELSDYPAITVAVDNTIDDTG
                     PSIVCLRPEDLLVTGATEERPLGQCWTGRVEVASYQGASIRYRVTLDNGPEIEAVATG
                     HGSPVLGIGEQAYVTARPGAAQVLLDDRAEAPASVTA"
     gene            complement(18467..19576)
                     /locus_tag="VFC01_07090"
     CDS             complement(18467..19576)
                     /locus_tag="VFC01_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015889072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HZN79439.1"
                     /translation="MRGGTSKCWLFNAVDVDPLVQRTGDLDGLLTSAFGSGDPLQLNG
                     VGGGSSTTSKAAIVRRSAEPDIDVDYLFAQVAVGQRQVEWGSNCGNCATAIALYALQA
                     GMVAVDRGRTTVRMRNQNTGSVLAAEIDTPNASIPTDGIAAVPGTSALGVPVSLTFAG
                     VVSSSALLLPTGNTQDLLVAQGREYRGTLVAAGAPAALFYAEDFGMTGAEGNEAITER
                     LPVLVELRRQAALAMGLSRPEDPVVHAVPKVGIVGAPTEYRTSTGAVVGAEDYDVSVR
                     MVSMLAPHPAIGLTSAVAVAAAAAVPHTTVAERTRAGLPGELRIGTAAGVIGTTITTS
                     ADGIPLTVTLHRAARRIATAELFIAESTPSLAGSR"
     gene            19751..20659
                     /locus_tag="VFC01_07095"
     CDS             19751..20659
                     /locus_tag="VFC01_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015889074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN79440.1"
                     /translation="MHSLDPRRVLLLVDVVRAGSLTAAAARLDYTTSAISQQIAKLEA
                     EAGQPLLERHARGVRLTEAGSALVRRAEKIETHLQAARNELDDIAGLRSGTVRIGTFP
                     TAGASLLPRVVKLFKSRHPAVALTVRSSRFAALRAMLDSREIELSLLWDYEWNPVTDP
                     ELTVRQLLIDPPALLVSANHRFAKRRSINMTELAGEQWITREDSHPVGAALERACSAA
                     GFSPTVAFAANDYQEAQAMVAVDLGVSMAPRLALSNLRDDVRVIPLRGAPSRRILLVH
                     LAEHRLTPAEAQLSGIFADVARNWRR"
     gene            complement(20667..21092)
                     /locus_tag="VFC01_07100"
                     /pseudo
     CDS             complement(20667..21092)
                     /locus_tag="VFC01_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730385.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(21235..21795)
                     /locus_tag="VFC01_07105"
     CDS             complement(21235..21795)
                     /locus_tag="VFC01_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN79441.1"
                     /translation="MDGKGQDRILDIVVELLETEGYDAVQLREVARRARTSLATIYKR
                     YGTRDELILAALNSWMEENRYSGLTKPHDADESLYAGLMRVLRTIFEPWERHPAMLNA
                     YFRARSAPGGQKLVRRGFDAVVPAAMEVLADADPAFVKDVDDIVSSAVYGLMGRFAAG
                     EIDITAILPSLDRTVHYLTAGYEASR"
     gene            21857..22687
                     /locus_tag="VFC01_07110"
     CDS             21857..22687
                     /locus_tag="VFC01_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZN79442.1"
                     /translation="MGSLDGKVAFITGVARGQGRSHAVRLAADGAAIIGVDICADIAS
                     NGYPMASREELDETVALVEAAGGKMVASVADVRDFHALKAALDAGVEQFGRLDIVLAN
                     AGIATMAFREVTDDEDLEMWTDVLNVNLVGAYHTAKAAIPHLIAGGHGGAIVFTSSTA
                     GLRGFGGLQGGGLGYAASKHGIVGLMRTLANALAPQSIRVNTVHPTAVNTMMAVNPAM
                     VSFLENYPDGGPHLQNPMPVELLEPEDISAAIAYLVSDAAKYVTGVTFPVDAGFCNKL
                     "
     gene            22684..23487
                     /locus_tag="VFC01_07115"
     CDS             22684..23487
                     /locus_tag="VFC01_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZN79443.1"
                     /translation="MSGRVEGKRVLITGAARGMGRSHAVRLAEEGADLILVDICESIP
                     ELEYPLSTREDLDETARLAEKHGGRVVTGVVDVRDADALAAVVNDGVAELGGLDAAVA
                     NAGVLTVGTWDQTTQQHWRTVLDVNLIGTWNTCAAALPHLVDRGGSLVNISSAAGLKG
                     TPLHLPYTASKHGVVGLSRALANELAAQNIRVNTVHPTGVETGMRPENLQQILGEGRP
                     DLIPIFLNALPAVMAEAIDISNAVLFLVSDESRMVTGLEFKVDAGVTIR"
     gene            23484..24290
                     /locus_tag="VFC01_07120"
     CDS             23484..24290
                     /locus_tag="VFC01_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN79444.1"
                     /translation="MTTVDIDRSERGRREFAEVMTFDPPDDSSPSTTNGLIDFVFGEV
                     WSRPGLSRRNRRFITLPCVAAADAEGPLKEHVYAALNSGDLSITEMRETVLHFAVYSG
                     WPKASRFNMVVDEQWERIHRERGHTVPPPDPLLPLVTPSDPEERLLTGEQSFKDINCL
                     PFAPQRDNPYQGAGILNFVFGEMWLRPGLGMKERRLVTVACVAFQDAPYPILSHVYAA
                     LKSRDVSFDEMDELALHFGAYYGWAKASHLNQVIGEQKQRVLDEWRQEAQ"
     gene            24269..25735
                     /locus_tag="VFC01_07125"
     CDS             24269..25735
                     /locus_tag="VFC01_07125"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN79445.1"
                     /translation="MAPGGAVTATLAGGLLIGGDRLTETSGGTYAHIYPATGQPNAHI
                     PLAGADEIDRAVASAWDAHREWMALTVDRRRDLLIDLADAVHENLDELSRLNVHDYAV
                     PISFASNAIMLERFLRHFAGYVDKPHGSSTPVTGSFDVNLIEREPYGVVGVLTPWNGS
                     LVVTGSCVAPALAAGNAVVLKPSEVAPLAALRFGELCLEVGLPAGLVNVVPAGPEGGD
                     ALVRHPGIRKIHFTGGDATARNVLQSAAVNLTPVVTELGGKSANIVFDDADLDAAAML
                     SAHQGPLMQSGQSCACASRVLVQESAYDAFLEKFVAVIEGSTVGDPLNPEVVFGPVVS
                     QPAADRILGVIEQAVSERSGELLVGGTRLGGELADGYYIQPTVFGDVDNRSALAQNET
                     FGPVVSVMKFSDEAEAVRIANDSRYGLNAFVQTNDLRRAHRVARQLEAGSVWVNTFSD
                     ISPQGPYGGYKQSGFGRTGGLEGLHEFLQVKNIRIGMQ"
     gene            25827..26663
                     /locus_tag="VFC01_07130"
     CDS             25827..26663
                     /locus_tag="VFC01_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZN79446.1"
                     /translation="MAGRVEGKVAFITGAARGQGRSHAVRLAQEGANIIAVDICQPFA
                     DSPAPAATPEDLAETADLVKALDRRIVTAEVDVRDYDALKAAVDSGVEQLGGLDIIVA
                     NAGIGTVGTRLERMKEDIWQEMIDVNLTGVWKSVKAGVPHMLAGGRGGSIILTSSVAG
                     LKTYPHTGHYVAAKHGVVGLMRTFAVELGAQSIRVNSVHPTHVNTPLLHNEQTYRMFR
                     PDLENPGPDDMAPVCQSFHMLPIPWVTAEDISNAVLFLASDEARYITGVALPVDAGSC
                     LK"
     gene            26684..27886
                     /locus_tag="VFC01_07135"
     CDS             26684..27886
                     /locus_tag="VFC01_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN79447.1"
                     /translation="MTIAHDTDVYYDPYDIDIVNDPYPVYARLREEAPIYYNERFNFW
                     AISRYADVEKALADWETFSNSRGDILELIQSNFDMPKGVMMMEDPPVHTMLRGLMSRV
                     FTPRRMAEIEDQIRRYCVNCLDPHVGSDGFDIIAELASMMPMRVIGMLLGIPESEQIN
                     VRDANDANLRTKPGAPMKVVTADKIADGRIYADYIDWRAKNPSDDLMTALLNVEFEDE
                     NGVTRKLTRDEILRYTQVVAGAGNETTGRLIGWLAKVLAEHPDQRREIEQDRSLLNRA
                     VDETLRFEPTGPHVARWMARDFECYGTTVPAGSAMLLLFGAANRDSRRYKDAETFNIH
                     RDNISHLTFGKGVHYCLGANLARLEGRVALDELLNRWPEWDIDYETAQLAPTSTVRGW
                     ERLRLVLP"
     gene            complement(27925..29433)
                     /locus_tag="VFC01_07140"
     CDS             complement(27925..29433)
                     /locus_tag="VFC01_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZN79448.1"
                     /translation="MTRLSAREVIDALIDAGSWISWDGALRETPADAEYGDELACARR
                     KTGLDESVLTGSATIRGHRVAMLVCEFGFLGGSIGVAAGHRLTTAIRRATAERLPLLA
                     LPTSGGTRMQEGTTAFLQMVRITAAVTDHKAARLPYLVYLRHPTTGGVFASWGSLGHV
                     TFAEPGALVGFLGPRVYEALYGDPFPTGVQTAENLFRCGLVDAVCPPNELAEVVARTL
                     NIVSGSSNGGGVASICYRGARSFPAWTSVVASRQPDRPGVQEILSTATNVVPLRGTGQ
                     GEADPALIVALVRFSDVSCVLLGQDRRAPANAPIGPAALRQARRGMRVAVDLGLPLVS
                     VIDTPGGELSRAAEEGGIAGEIARCIADMLALEVPTVSLLLGQGTGGAALALFPADRT
                     MGAANGWLSPLPPEGASAILHHSTDRAPEVAARQGVRAADLLASGALDSVIPEYPDAR
                     AEPEQFSRRVAAAIRRELASLVTVDPTTRLRRRIQRFDGLATPAPPAAAEIA"
     gene            complement(29430..30638)
                     /locus_tag="VFC01_07145"
     CDS             complement(29430..30638)
                     /locus_tag="VFC01_07145"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN79449.1"
                     /translation="MTSTIPSSTRPAGPLAGLVVVDLTRALAGPHAAMMLGDLGAEVV
                     KVENPIGGDDTRGWGPPFVDPAHGERESTYFLCANRNKKSVTLDLKEETGRAALRALV
                     RRADVLMENFRPGVLDRLGFSQQQLAEINPRLVVLSISGFGHDGPEAGRAGYDQIAQG
                     EAGLMSVTGSDPTDVQRVGVPIADLLAGMYGAFGVLAALQERERTGRGQVVRASLLAA
                     VVGVHAFQGTKWTVAGQVGEATGNHHPSICPYGLFDTADGAVQISVGSEGLWKRLCEG
                     FGFDADAAGMATNPERVANSKRVREMVQARFAFYNTDELLAKLDRIGVPAGRIRTIRE
                     VYEWEQTKSQGLLIDVDHATLGSITLPGPPLRFFDAEGAELTRKHHTAPPVLGADNDL
                     IEATILADHR"
BASE COUNT         5491 a         9687 c        10111 g         5389 t
ORIGIN      
        1 ctgccaagtc ttttcgaagc gcgggctgtc ggttgacctg ggcggctcgt ggctgctgcc
       61 gaaactggtc ggactgcagc aggccaaacg ccttgtgctg ctcgccgaga cgatcgacgc
      121 cgacgaggcg cggtcgctgg ggttggtcac gtgggtgaag tcatctgggg agattgacgc
      181 gttcgtctcc gacctagcgg ggcgactcgc cgcaggtccg cctgtcgcgc tggcacagag
      241 caagggcctg ctcaacgatg gcgccaatgc gaccctgcgc gaggcgctag ccaacgaggc
      301 ccgcgcacaa cccggcaact tcgccaccgc agactcaata gaggcgtacg cggcgttcgc
      361 cgagaagcgc gaaccagcgt tcactggacg atgggcagta ccacccagat cggagtgaga
      421 tagatgcgtg aaaccgtaat cgtcgaggcg gtgcgcacgc cggtcggcaa gcgcaatggc
      481 ggactgtccg acatgcatgc cgccgacctg tcggcgatcg tgctcgatgc gctggccgaa
      541 cgcgcaggca tcgaccccga catcgtcgac gacgtggtgt ggggttgcgt ctcgcaggtc
      601 ggcgaccagt cgagcaacat cggccgctac tcggtgctgg ccgctggttg gcccgagagc
      661 attcccggta cgaccatcaa ccgcgcctgc gggtcgagcc agcaggcgct ggacttcgcg
      721 gtgcaggccg tgatgtccgg ccagcaggac gttgtcgtgg cgggcggcgt cgaggtaatg
      781 agccgggtgc cgctcggttc ggcgcgggcc actggcatgc cgtatgggcc gaaagtgctt
      841 gcccgctatg gggatttttc attcaatcag ggcatctctg cagagctgat cgcgcagaag
      901 tgggggtttt cgcggacccg cctcgatgag tactcggcgc gctctcatga actggccgcc
      961 aaggcgcagg acgacggcgc gttcgagacg cagatcatgc cggtgttcac cggcggcgag
     1021 ccggtggtgg ccgacgaggg catccgccgg gggacgaccg tcgagaagct cgcgggcctg
     1081 aagccggcgt tcaaggagga cggcgtcatt cacgcgggaa actcgtcgca gatctcggat
     1141 ggcgcggcgg cactcctggt gacgacggca gagaatgcga tcacgatggg actgacgccg
     1201 atcgtccgtt accgcgccgg tgccgtgacc ggagccgatc cggtgttgat gctgacaggt
     1261 ccgatcccgg ccaccgagaa ggtgttgcac aaggccggcg tggggttgga cgaggtcggc
     1321 gtgttcgagg tcaacgaggc gttcgcgccg gtgccgctgg cttggctggc cgaaaccggc
     1381 gccgacgaag acaagctcaa cccgctgggc ggggcgatcg cactcgggca tccgctcggc
     1441 gcgtcgggtg cggtcctgat gacgcggatg atcaatcaca tgcgcgacaa cgggattcgc
     1501 tacggcctgc agaccatgtg cgagggcggc ggcacggcca acgccacact ggtcgaactc
     1561 atttcgtaat aaaggagttt ggatgcgccg cgatctgttc accgaggacc acgaagcgtt
     1621 tcgcgaactc gcccgcgact tcgtcgagaa ggaggtggtt ccgcactatc ccgagtggga
     1681 gaagggcgga cggatgcccc gcgacgtctt caagcagatg ggcgcgctcg gcatgctcgg
     1741 gatggccatt ccagaggagt acggcggcgg gggcgcaccg gactaccgct tcaacgtcgt
     1801 gctgcaggag gaggcggcgc gggcgctcgt cacgctgtcg accgtgcgca cccaactcga
     1861 agtgatcctg ccgtacttcc tgcactacgc caacgaggag cagcgcaagc gctggttccc
     1921 cggcctcgcg gctggcacgc tgctgaccgc gatcgcgatg accgagccgg gcaccggctc
     1981 tgatctggcg ggcatgcgca ccaccgcagt gcgcgacggc gacgactgga ttctcaacgg
     2041 cgccaagaca ttcatcaccg gcggcatgca ggccgacctg gtgatcgtgg tggcccgcac
     2101 ggcgaccgat ccggacaacc gccgcaaggg cctcaccctg ttcgtcgtcg aagacggcat
     2161 ggagggcttc acccgaggcc gcgagctgga gaagatgggc tgcaaggtgc aggacaccgc
     2221 cgagctgtcg ttcgtcgatg tccgcgtccc cgcagccaac gtgttggggg aggagggcga
     2281 ggcgttcggc tacctcggtc acaacctccc gcaggaacgg ctcaccgtcg cggtcgggtc
     2341 ggtgtcgcag gcccgctcgg cgatcgccgc agcgatcgac tacacgaaga accgcaaggc
     2401 gtttggcacg ccggtcgcgt cgttccagaa caccaagttt gagctcgccg cgtgttcgac
     2461 ggaggtcgag gccgcgcagg cgatggtgga ccgcgccgtc gccctacacg tcgagagtga
     2521 gctgtcagcg gccgacgccg cccgagtgaa gctcttctgc accgagatgc agcagcgcgt
     2581 ggtggaccgg tgtctgcagc tgttcggcgg ctacggctac atgatggaat acccgatcgc
     2641 ccggctgtac acggacgccc gggtggcccg catctatgcg ggtaccagtg aggtgatgaa
     2701 ggtgatcatc gccaaatcgc tgggccttta atgcctttcg tcgtcaggcg cgggcgatgt
     2761 tctcgacgat gatatcgcac gccgcctcgt agaacgcact gatcagggtc gagaaggaca
     2821 gttcgaacgg atagatcatg tgcacctcca acggatccgg tttccattgt gggagcacgg
     2881 gcacgatgct gccgtcacgc agctcgtcag tgcagatgat ctcagtgggc atctcgccga
     2941 taccgagccc ttgcaggatg tactgcttgc acacctgaaa gttgttggcg cgtgcccgta
     3001 tctgcggagc cagctcgtat cgccgcttgt ggttgaccac cgtgagcttg tgcggcccgc
     3061 ggaagccgaa ttcgatgaag ggcaagccgt tcaactgcac cggatcggtg atcggctccg
     3121 tcagctgctg gacgaaatga ggtgacgcgc aaaggaagag ctcgaggtcg aacactttgc
     3181 gagcgatcaa cgtcgaatcc tgcaacggcc cggtaccgaa caccacgtcg aaaccgtcct
     3241 tgattggatc gaccatgttg tccaccagcc ggatgtccaa ccgcgagtgg gggtaacgct
     3301 gcagaaacgt ggcaccgacc ctggacgcgt agtcgacacc gacgaagacc ggaatggcga
     3361 cccgcaattc gccctgcggt tcctgcctgc tgccttccac cagcgcgcga acaccgttcg
     3421 cttcggccag gatgttcacg gcgtggtcgt acaccttctc accgaggtcc gtgacggtga
     3481 gctgatgggt gttcttgcgt agcagcttga tgccgaggtc ggcttcgagc ttcgtgagct
     3541 tgcggctgac cgtcgacttg ggcattccga gtaacgccgc cgccttcgac aggctgcggc
     3601 tctccaccac cttgccgaag accagcaggg cgtcgacgtc gaagggcagg ttctgttcca
     3661 tggtctctgt tccaaatatg caacagcgtg ttgcggtttt gcgtctgttt tgacgatttt
     3721 cacgctgtta gcgtcggcgc ggaggagggc cccatgagtc acaacagcct ggtgaccaag
     3781 cccgcgagtg gacactggtc ggatgcgtat cccgaactcg ggcgcggtcc ggtctcgctc
     3841 gaggactgcg tatcgccgga gttctacgag aaggaacgtg agcacgtctt cgagaagacc
     3901 tggctctacg tcgggcgggt cgagcgggtg ccgaagtccg gaagttactt cacgcgcgag
     3961 ctgagattcc tcaacacctc ggtcatcatc gtgcggggca aggacggcgt gatccgcgcc
     4021 ttccacaaca tctgcccgca ccgcggcaac aagatgctct gggaggacga ccccttccag
     4081 gaggtgtccg gccgcgcacc gttgctgtac tgccgcttcc acggctggcg ctacaagctc
     4141 gacggctcac tgcactcggc aacccgcaag gacctgctgc tcgacttcga tgccgacagc
     4201 tgccgggtgc cggcgattca atgtgaagtc tgggaaggct tcattttcat caacctcaac
     4261 ccgcacaaca ccgagccgct gcgttccttc ctcggcgagc tggcctacgg catcgagggt
     4321 tatcccttcg agggaccaca tcaggtctat cggttcaaca ccgaattgca gtgcaactgg
     4381 aagattttcg tcgatagctt cgccgagagc taccacgggc cgtatctgca tgcgtcctcc
     4441 ttcggcgctc tcactgcaga ggccagagat gctttcgatc aaccgaaccc gttcaccgat
     4501 gcgttggcct accagctcaa cgggccgcat cggatgttct cgttctccgg agagccgtcg
     4561 cagaagacgc cgtactccaa gccgatcgag tgcgtgatgg aggccagcgc tgccggaccc
     4621 tggaacaagc gcaccgatcg cggaccgatg ccgcccgggc tcaatcccac gcgctcagag
     4681 aaatacggtt tcgactccta ccagttcttc cccaatttcg tgctgatctt cggcgcgtca
     4741 ggcttcagca ctcacgtcca ctggccgacc gggccgcact cccacatctt cgaagtcgag
     4801 atgttctatc agcccccgaa gtcgcacaag gaacgtctcg gacaggagct gacggtcacg
     4861 ttcctcaacg acatcatcct ggaggacgcc agcccgtcag aaggcttgca ggcgatgctg
     4921 aacagcggtg cgctcacgca tttcacaatg aacgacgagg agatcctgct gcgtcacctg
     4981 cacaagacgg tggccgacta tgtccaagca ggcgaggccg cacgatgagc gcccttctgc
     5041 cgccggagtt ttcgcaactc gaccacttgg tcggtgaatg ggccatcgag gatgggcacg
     5101 cacgttacgt gaagcgcgtg aacagttcca tggaacagat ccaggcgttc tacgacgagg
     5161 tgtttccggt ggccgaggaa gcggtcgcct acatcgacaa gttcgactac tccgaaccgc
     5221 tgcccgacga cgtcgccaac ctgcgcaacc tgctctactc gctgatcacc gtctcgttag
     5281 cggtggaact atggaaacaa ccgcgggtaa agcattcggc caacaccatt cttacgagag
     5341 tgagctgatc tgccatggca ctgacctctg cacaattcga catcgtcgac cgcgccccgc
     5401 ggatcggcag cgagatcagg accgacctcg aaacgctgct cagcggtcgg gaagccgcga
     5461 acatccgcga gacgctcgaa cagcggggcg tggtgttctt ccgcgggctg gacatcagcg
     5521 acgagcagca agtcgccatc gccaaaaccc tcggcaatat cgtgcggaac gagggcgaca
     5581 gcggcatcta caagatctca ctcgacaaga acgtcaacca gcgcgccgag tacctgaagg
     5641 gctcgatgtt ctggcatttc gacggatcgc tgcagccgta cccgaatctc gcgaccctgt
     5701 tgcgggcgat caagctttcc gaaaccggcg gcgataccga gttctgcaac acctatgcgg
     5761 catacgacga cctcccgcag gccgacaagg atctcatcgc ggaccttcga gtcgtccaca
     5821 gtgctgagcg ctcgcagtac tacgtccgtc ctgagatgag ctacgacgag atcaccttct
     5881 ggcagaagtc accgacgaag gcatgcccga tggtgtggac gcaccagtcc gggcgcaagt
     5941 cactgctcct cggcgcgacg gccgattacg tcatcgggct accggtggag gagagccggg
     6001 cattgcttgc ccgccttcgc gattgggcca cccagccgca gtacgtctac cgccacgagt
     6061 ggcaacccgg cgacctgctc gtgtgggaca acaccggcac gatgcatcgg gtgctcgagt
     6121 atccggtgga cagcggccgt cttatgcacc gcacgatcct cgcgggcgaa gagccgctgc
     6181 aatgaaactc ggcattgcca cgcccgtcgt caccaacgtc ggaggcgccg cgctgacgtg
     6241 ggagaaggac gcgacgatcg aggacatcgg ccgggtcgcc gagaccgcgg atcaacttgg
     6301 ctaccaccat atgacgtgca gcgagcacat cggcctgccg tcgtctgaaa ccgcgcgtcg
     6361 cggtgcccgg tattgggatc cgctggccac gttcggtttt gcgtcggctc gtacgaagca
     6421 gatccggttc gccacgatga cgctggtgct cgggtatcac catccgctgg ccatcgtaaa
     6481 gcgctatggc acattggatc acgtcagcaa cgggcgggtg atcctcggcc ttggcgtcgg
     6541 ctctctgaag gaggagttcg accttctcgg cgcgccgttt gacgatcgtg gtgcccgcgg
     6601 cgacgacgcg ttgcgggcgc tgcgggcagc gttgccgagt aacaaaccga cctacgaggg
     6661 cgagttttat tcgttcggcg ggctcaccgt cgatccgtgc gccttgcagc ctcacatgcc
     6721 gttctggatc ggcggccgca ccaagcggtc gttgcggcgg gcggtaacgc tcgccgacgg
     6781 ctggtgcccg ttctacgtct cgatcgacac ggccgcggag tggctgaaaa cctttgacgt
     6841 gccgcccggt ttcgaggtgg tcatgcccgc cgaccagccg ctggacccga tgggcgaacc
     6901 aggggcgacc aaggagacgt tggaaacgat ggccgcgggc ggcaccacga cgctgtccgc
     6961 ccgcttcgtc catcattcgc tcgagcacta cctcgagcag atccacgccc ttgctgaaat
     7021 gcacgatcct gccggagggc ggtgcacatg acgggtccgc tcctgtcgcc gctgtccgcg
     7081 gatgaatgga cagatgacgc gtacgccgca gtcggggcgc tgttcggcgt accgggtgag
     7141 gccatccctc gggccggctc gggggaggcg cgggacccgc tcaacttcga catcctaggc
     7201 gtgctggccc gtcatccgga tctggcccga gtttttctga gctacaacgc ctttctgttg
     7261 cagcgcggcg agttgcctct gcgcctgcgg gagttggcga tactgcgcct ggctcacgtt
     7321 cgacgctcgg cgttcttctg gggtgaacac gtcagggtcg cgacggcggg cgggctgtct
     7381 gaagacgaca tcgccggcct tgcccgcggc aacgttgagt tcgacggtac ggaccggctg
     7441 gtgctcgagg cgacggacga gatgctcgcc gacgggcggg ctcggcccgc gatgtggaaa
     7501 cgcctcgtcg aagagcttgg cacgcatcag gcgatggaac tcatcttcgc cgtcggcacc
     7561 tacggaatgt tggcgatggc gtgcgacacc tggcagctcg ttccgccacc gggcagcgcg
     7621 aaacttcccg cggaaacagc acgcgatgac gactagccgg ctgaagagga cggtcgctcg
     7681 gccgcactga tctaggtgct gttggccgaa caaatccgct gacctgcggt gtgacgacgg
     7741 tccaatcctt ttttcgctga gacccttgtc acaggccgca aacctaccta ctgtgtgttc
     7801 actaggttgg ttgaccgaag gggttcagtg acgtcggcat cgggttcgag gccttacgcg
     7861 acgctcctcg ccaagggcga ggaccgcagg cagcggatac tttccgtggc cgaacggctg
     7921 cttgcccgca acggctggcg caacacgtcg ctggccctga tcgccaagga agccggggtg
     7981 acgcccgccg gactgctgca ccacttcgag tccaaggagc agctgctcaa cgcggtgctc
     8041 gacgcccgtg acgcggatga cgatgcccat gccgactacc gccgcggcga cctcatcacc
     8101 gagctcagcc gggtacccga gcggttcgaa cgcgccccgg agctgatcgg cacgttcacg
     8161 gtgctgttgg tggagaacat cgcgccggac gccccgctgc acgaccgcat tcataagcgg
     8221 taccgcgatg caatcgacat catcagcgac atcatcacgc ggggtcagcg agacgggctg
     8281 taccgcacag acttcgacgc ggccagcaag gccgtggaaa tactcgcctt catcaccgga
     8341 atggagaccc tatggttact cgatccttca ataccgctga ccgaggtttt caaggggtac
     8401 gcggagtcgc tgggacgcga gatcgagcca aggagctcga catgaggtat cggcttgacg
     8461 ttgtcgcacc caccgtgctc gacgcggtgc ggttcgccgg cggctgggtc tacgaccgcg
     8521 tgatggcggg ttgggacgtc accgttctga tcggtggaga tgacgacgtg cggccgttgg
     8581 agattctcgg cactgaggtg cgcgacctgg agtcggtgct ggagtcgtgg gaggaccgtc
     8641 cccatccgca gaccgttgcg gtcgcagggg atctgttcgc ggtggacgct cgtgtccgcg
     8701 gacacgtgtt gaatgcgctc gatcagggcg caaccgaagt gacgctgtgg ggcgacagcc
     8761 tacccgccga gctcgatgac agtgtcgact cggtgcagca ccgattgagt gcggccgcac
     8821 gggctttcaa ggcgcaggcg ctcgcggcgg cgaatggtcc ggcgggggat gtcggtcaga
     8881 ccgaaacgtt ccgatgcgga aggatggcct cggtcgccgc ggatctgatc ccggcaagtt
     8941 agcgctcgca tattaggtga ggtcgatcaa gaccttgccg atggcgcggc cgtcggcgac
     9001 atggcgtagc gccgctgcgg tttcggccag cgggtagacg gcacagacgt gtggcagtac
     9061 gcggccgctg acgaggagtt cgcgtaattc gttgtcgttg cgggcgaatt catccggcgg
     9121 gacgtcctgg aactggaacc cgaggacgtg gatgcccttc accagcacca ggttcaacgg
     9181 gatgcggggg atgacgccgg aggcgaagcc gacggtgacg aatcgcccgc cgcggcccag
     9241 cgaccgcaac gcgggttctg acagctcacc gccgacggga tcgacgacca catcggcacc
     9301 gccgggcacg gcctccttca acgcggcgcg tacgtcggct gctttgtggt tgatgacgtg
     9361 cttggcaccg taacccgctg ccacatcgag cttttcggcc gacgatgcga cggctgtcac
     9421 cgaagcaccg agttgcactc cgagctggac tgccgccagg ccgacgccgc cgcctgcgcc
     9481 cagcacaact aactcgtcgc cctcctgcac ccgggccatc gaccgcagcg tgtggtacgc
     9541 cgtgcgatac gcgacgccaa acgccgccgc agtgcgatca tcgacgccgt ccggtatccg
     9601 ggacagccca gcggcgggca ccgccacctc ttcggcgaac gcgccgaaca acccggtccc
     9661 ggtgacccgg tcgccgaccg cgaatcccac tgcaccttgg ccggtttcga cgatcgcgcc
     9721 tgcgaactcg ctgcccggca cgaacggcgg cggcacgctg atctggtact cgttggcgat
     9781 cagcagcacg tcggggaagt tcacggccgc cgcaccgacc ttcacccgga cgtgaccaga
     9841 ctcgatcgcc ggcgacggct gctcctcgat acggacgacg tcgggtggcc cgtaggccgg
     9901 gcagacggct gctctcactg gtagtcggtg gactcggcga ggtcggccgc cgagcgcatc
     9961 agtgtcgtga tgacgggacc gtaggcgagc aacttttcgt tgtcgcccgc ccgttggaac
    10021 ccctgctcca acacgatcgc gagcttccac ttggcgagca ccaggtagta gtcgaggtca
    10081 tcgacctgac gccccgacac ctcggcatag tggtcgacca cctgtgtgcg cgaaggcatt
    10141 ccgcgcatgt cgacatagct catcgccgat ggtgcatcgg tgtcttccgg ccagctctgc
    10201 accatccaag cgagatcgag cttcggatcg cccacggtgc ccatctccca gtcgacgatc
    10261 gctgccatct gcgcgggcgc gccgtgtctg tacatgacgt tggcgaactg atagtcgccg
    10321 tgcatcagac cagggatgaa gtcgatcggc cgatgggcgc gcagccattc ggtggcgaca
    10381 tccagtccgt cgatctcgcg tcccttgatc cgctcgaaga aggcgatcca ccggtcgact
    10441 tggcgctcat ggaagccgtc gggcctgccg aggtcctgca gcccttttcc tttccagtcc
    10501 accttggaca acagcgcgat gccctccgcc agctggtaag acagtccggc gcgcgcctcc
    10561 atgtcgctat cgaacggctc gggccagcgg gcgtgggtgt ccatcggcga ccagccgtca
    10621 acgaagccca tgaggtagaa cggacgcccg agcactgcgg cgtccgagca caccccaccc
    10681 gccttggtgt gtggcacgtc ggtgccgtcg agcgcctcga tgatgcgcca ctcacgcagg
    10741 atgcccttgt cgcggtcggg cggggcatcc ggcggcggca tgcgaagcac gcactcgtgc
    10801 tcgccgcggt tgaccttgta gatcacgttc tgcgtgccgc cggagaggaa ctgcgcgtcg
    10861 agcggctcgc ccttgcccgg cagcgcctcg ccgtccatcc aatcggcaag gcgcgcaacg
    10921 tcgatatcgc tcacagattc cccacttcgt tctccaggta gtcggcgtat ttggcccgcg
    10981 cggcttcccg cttgcgcggg gtccactcgg tcggccacgt gtcgtcggtg gcctggtagc
    11041 cgcgaagcac ctgcttggcg accgtcgtct tgtgcacctc ggtgggaccg tcggccagcc
    11101 ccatcaccgc ggcgccggtg accatgccca tgaagggcat ttcgttggtg acgccgagcg
    11161 caccgtgcac ttgcattgcc cgccaggcga tgtcgtggag cacagtcggc atcacgacct
    11221 tgacggcggc gatgtccttg cgcaccttct tgtagtcgtt gtatttgtcg atctcccaag
    11281 cggtgtagag caccatgagc cggaactgca gcagttgcgc gtacgagtcg gcgatgtagc
    11341 cctgcacgaa ctgcttgtcc gacagcaggc tgccctgagt ctggcggctc aacgcgcgtt
    11401 cgcacatcat gtccagcgcc ttgtgcgaca ggccgatggt gcgcatcgcg tggtgaattc
    11461 gcccgccgcc caaccgggtc tgggcgatca cgaatgcctg cccctcgccg ccaagcagcg
    11521 cctcggctgg cacccgcacg ttgtcgtagt ggatcagcgc gtgtgaaccc tcgtcgtcgg
    11581 gctcgccgtg caggccgacg ttgcggacga tgttgacacc cggcgtgttg gcgggcacca
    11641 ggaacatcga catgccctgg taggcgctga cgtcaggttt ggtcaccacc atcactatta
    11701 gaaacgacgc ggttttggcg ttggaggaga agaacttcca accgttgatc acccagtcgt
    11761 cgccgtcgcg gacggcgcgc gtcgtgaaca gcgtcgggtc cgcaccggcg tgtggctcgg
    11821 tcatcgagta gcaggagaac aactcgccgt ccagcagtgg tcgcagatac ttctctttct
    11881 gctcgtcggt gccgtaatgg gcgatgatct cggcgttgcc ggtgtcgggc gcctgacaac
    11941 cgaatacgat cggagcccac tgcgagcggc cgaggatctc attgagcagc gcgagcttga
    12001 gctggccgta gccttggccg cccagctcgg gtccaaggtg ggtggcccac aagccttttc
    12061 ggcggacttc ttccttcagc ggatcgatga ccttgcgccg actgtcatcc agcggcacga
    12121 attgcaggtg cggaaacgcc agatcgagtg gttcgcactc ctcgcggacg aactcgtcgg
    12181 cccagtccag cagcttctgg tactcggggt cggtttcgaa gtcccacgcc attgcgggct
    12241 cctctcagtt gaaggtgacg acgaccttgt cggccgcgcc gggcgtcgtg gcggtcttga
    12301 gtgcgtcgtt gacgtcatcg aacgggattg tgtggctgat gatcacggcg tacttctccc
    12361 aattggcgat cagatccttg gtgacctcaa agatctcgtt gggatagccc attgacccga
    12421 cgatggtgat ctcgttgctc atcaggttga ggaaatccac cgacaccggc tgcttgtgca
    12481 ccgcgacgat cccgagccgg gcccctgtct tggcggcggc gaaggccgtc tcgatgaccg
    12541 ctggcacccc ggcggcgtcg aaatagatgt ccgtaccggc ctttccgccg aacatcgagg
    12601 cgccttcacc gtggagttca agaagatgct tggcgacgtc gtccgcggcg gagttgatca
    12661 ccgcgtcggc acccactttg agggccttct caaggcgagc gggaatcagg tcggccacca
    12721 cgacgtggct gacgccaagc gatttgaacg caatcgtggc gcccagccct atcggccccg
    12781 cgccgaagat catcaccttg tcgccgggct tgggccggca ttgattgacg ccgtggcgcg
    12841 ccacggccat cggctcgttg agcgccgcga cttcgaacgg gatgtggtca ggcacgactt
    12901 cgaggctggt gccgcgtacc gcgttttcga tcaacaggta ttcggacggc gcgccggcgg
    12961 ttgcgccgtt gccgatgatc tcgcccgcct tggccatcgg gttgatcacg acatggtcgc
    13021 ccacggcgat gccggtgacg tcgctgccaa cctccgcgac ctcgcccgcg ggttcgtggc
    13081 ctatgggcat gccgtcgtca tggccgggga tgccgcccat cgagatgaaa agcgcgtcgg
    13141 taccgcagat tccgcatgcc cggatcttga cgagcacatc ggcgggcccg accgtgggct
    13201 tcggcgcgtc gatcacctgt gtcgcaccag gcgctgtcac caccgacacc ttcatctgca
    13261 cgctcctcta cgcggggagt tggttgtagc cggcgatcaa ggcgctgatg tcgcggccga
    13321 cgacgtccgc gaacgagcgg tcgccgtaag tgcctgcggc ccaatgccgt acaccttcgc
    13381 tgacgtgcat cccggcgaga tgggacagat gacgcacatc gacatgcgcc gccagcttgc
    13441 cgtcccgctg tgccatcgcg agcagatcgc cgaacatgtc ggcgtcagcg tcttcgagtg
    13501 gctcaccggc gaggatgcgg tgctcatggc ggtagccctc gaggattgtc tcgatgatca
    13561 gctcgcgcgg gttgcgcgcc atcgagcgtt ctaggctgcg cagcgcctcg gcgatcacag
    13621 cgtcgacctc atagtccgtc tgcagcagcg agcgcacctt cttctgcgcg gcgcgggtcg
    13681 acagcaggcc gacctcgaac agcacgtcct ccttggccgg gaaatagaag tagaacaacg
    13741 cgcgcgagac gcctgcggcg cggcagatgt cggcgacggt ggtcttcgcg tagccgtttg
    13801 tgcgccacag cgccatcgcc gcctgcacca gggcgcgctt ggtctcatgc gaccgggccc
    13861 gctgaaagga cgcacggcgc tgactaccgt cagcagctgt aacggcacca ttcctggcca
    13921 tgaacggact gtaacagcca tattcgctct tgcatatagt tgacggttgt ccaaccatct
    13981 ttgagtgaat gagcacggca tgatcgatgg ccgaggtaga ccgatcgcac cctccgagag
    14041 ctgctggacc aagttcggac actccgtggc tctgcaggcg ggcgacgacg tctgacaggt
    14101 tgtagccgtc tcgacggcgt gctgctcgac gttcgcatcg aggatcaggc ctgcgtgagg
    14161 gggtggtgat gggggcggtg acggccgagc gtcaaccggt gtcctggtgt cgggtgcagc
    14221 cgctcggcgc catgcgctac gtgcctgtcg ccagtccggc ctacctcaag cgttaccgga
    14281 cgaattcatc gcacacgcac ggtggcttcg ggccgtcgct ggtgcatctc gcatgctggg
    14341 actattcgtt gtagccgaag atgccattcc acgtcttctg atcttcggcg gcgttggcga
    14401 tggcttgcgt gggcgtaatg cggtgtacgc gagggtcatc gaagtcgggc agttgaacac
    14461 ctctcacgaa cggcgcgggt atgtcggatc gtacggagta ttcaccgatg tcgtgaagca
    14521 cccgctggcc ccgtttggac aggttccagt tcatgaatac ccttgcggca gcggtgtgct
    14581 tggccgtcgc tgcaactccg gtgtagtagt cgtaggcggt gacaccctcg gtgggcacaa
    14641 cgaaggtgac aggagcgttc tcgttgatgg ctatgttgac gctgctaacc acgacggtgc
    14701 ctgcatagat ttcaccacgg gctagggcat cgagttcggc gcccaacgag tcgaaaacac
    14761 gtgtgccgct ggagtactca tgtaggtagc cgtcgccgag gacttcacgc tggaaccggg
    14821 tcagcgccaa ggcgctcccg ccggcgcccg cctgggcgat gccaagcttg tcgtgccaac
    14881 gagagctgag caggtccttc cacgtttttg gcgcttcgtt ggcgtcgacg acagcggtgt
    14941 tgtagccgaa ggtgtagatg ggatcgaagg ttcggtaata gttgccgccg tcgaacacga
    15001 catcgtcgcg gaggctggtg gcggtgggtg gatggtaggg ctggaataca ccccgcttgc
    15061 tcaatccgct gacgaagccg gcatctgaga tacggatgac gtcagcgggc aacttgcccg
    15121 cgccgaactc ggccacgatg cgctcataga gccggttggg ggtcagccga atgagtttca
    15181 ctttgagacc ggtgtcggac tcgaactggt cgagcacggt ggcttcgctg tcctgtgtgt
    15241 aaccgctata cagggtcagc tggccttcgt ctttggcgtg agcccacagc gcggggtcgg
    15301 cgatctgttc gccgtcgacc accagcgcat gatctcgctg gattgactcc gcaggccggg
    15361 ccaacggcgg cgcctcggga ggcggtgagc aagccgccag cgcggcggtg atcgctgcga
    15421 cgacagcgaa gacgaagctt ttcatgagat ctccttggat ccaaagcgcc gggccaggac
    15481 ggcgagcaca ccgatgacgg cgcagtacag caagctcagc tcggccgcgg actgcaacga
    15541 cccgttgtcg taggcgtcga agatagcgat cgacagcagc cgggtgtcag atgtgaagag
    15601 gaagagcgcg gcggtcagtt cgcgcatcga caacatcagc agcagcagga acgtcgagga
    15661 caatccgact cgcagcagcg gggctgtgat agaggcgatc gctcggtgtc ggcgtgcgcc
    15721 aagcatcacc gcactgtcct caaggtcctt gtccaactgg atgattgagg ctgacacgcc
    15781 gcggtagccc tgcggcagga agaccgctac gaacgcgatc accagaacgg ccagcgttcc
    15841 gtagaccggg accggcaacg ccagccacgt ccacagcaag cccaagccca acacgatggc
    15901 cggaaccgcc agcggcagca tcgcgacgta ctccagcagt tttcgtccgg gcgccttcgt
    15961 acgatagcgg gtgtaggcca gggcaaagca aaacgccgtg ccgatggcag ccgtagcgac
    16021 acccacgacg acgctgttga gtacgacctg gtggaagtcc gaactctgga tcgtttggcc
    16081 cataccccat agcgacaacg caccgggttg cgtcagctgg ccgaggttgg cgacgtaggg
    16141 gctcgtctgc atcgcggcaa tgatgagcgc cccaacgggc agcagaacgg ccagcaagaa
    16201 atacgaccac gcgaatgcgg cagccggtag ccgccaggcc cgcagcggaa tgcgccgtgc
    16261 gcgaactcct ttgccgctga ccgttgtgaa ttccttgccg cccatggctt tttgctgacc
    16321 ggccacgacc gcgatcaaca ggacggtcag gaccaccgcg acggcggcgg catcgttgga
    16381 gcgcgccggg gaggcgttca tcagccggta gatcagcgtc ggcagggtgt ccaccccgcc
    16441 ggggacgccg atgacttggg cgaccgggaa gttctccacg gtgagcgcga acaccaggat
    16501 cgccgagccc agtagggcgg gcatcacgag ggggaaggtg accttgcgca gcatctggct
    16561 gatctttgcg ccgtgcaggc tggctgcgtc ttcaaggtcg gggttcatca aggacaacgc
    16621 ggagtgcacc atgaggaacg gatatggcgc gtagtacagc cccagcacaa acaccatgcc
    16681 gctgaagctg tacacgttga tcagatgggg gaggccaacg gcgttgagcg ctaagttgag
    16741 atatccggtg gcggggccgg ccagcagcgc ccacgccaag gcacccacaa gggcgggcaa
    16801 gaacagcggc gtgatcgcga tcccgtagat ggctttgcgc ccggggatgt cggtgcgtgc
    16861 ggccaggaaa gcgagcaggc agcccacagt gagagcaacg atcgaggaac cgatgccgac
    16921 ggccgccgaa ttgagcaacg cattcctgcc ggtacttgaa ccaatgacag cgaagttgtc
    16981 gagggtgaaa tgaccgaagt cgaacagcga ttcccgttcg gccttgtccg tgaacgcccc
    17041 catgacgata aagaacatcg ggagcagcac caggacggcc aacactgcca gctgcagcag
    17101 gaccggcagc cagcgcttcc cgtgaggtcg acgaggagcc actgcggcgc gttctgttgg
    17161 cggctttccg gtggtagctg ccggcgggtt aacggcgggg gcgctcatgc tgtcaccgac
    17221 gccggggctt cggcacggtc gtcgagcagc acctgcgccg cgccgggcct ggcggtgacg
    17281 taggcctgct caccgatgcc gagcaccgga cttccatggc cggtggcgac cgcctcgatc
    17341 tccggcccat tgtccagcgt cacgcggtag cggatggatg caccttggta actcgcgact
    17401 tcgacgcggc cggtccagca ctgtcctagt gggcgctctt ccgtcgcacc ggtgacgagc
    17461 aagtcctcag ggcgcagaca cacgatggaa ggtccggtgt cgtcgatggt gttgtcgacc
    17521 gcaacggtga tggcgggata gtcgcttagc tcgacgcttc cgggcccggt tcccttggtg
    17581 catctgaaga tattgccgat tcccaggaac tctgcgaccg atgcgcacac cggattggtg
    17641 tagatctgtt gaggagtgcc gatctggaca atgcagccct tctgcatcag cgcgatcctg
    17701 tcggcgagtg cgaatgcctc ggcttggtca tgagtcacgt agacggtggt cagtccgagc
    17761 cgaagctgca gttcacgtag ttccatgcgc agccgctcgc gtagccgggc atccagattg
    17821 gacagcggtt cgtcgagcag gagcacactc ggccgcatca ccaggctgcg cgccagcgcg
    17881 acgcgttgca tctgccctcc gctgagcagg ctggcccctc gctcggcgag ttcgccgaga
    17941 ccgacaaggt cgagggcctc gcctacacgc tgcctgagct cggacttgcc gactttctgc
    18001 atgcgcaacg agaacgcgac gttttcgaac accgtcttgt gcggccacac cgcataggac
    18061 tggaacacca tgccgacatt gcgcttatgc gggggcatgc tgcgaccact ggctgaatcg
    18121 tgtaccaccc ggtcgccgat ggtgatccgc ccgacgctgg gcgtttccag tccggcgacg
    18181 catcgcatgg tgctggtctt gccgcagccg gactgaccga gcagcacaag cgattcgccg
    18241 tcggcgatct ccaggtcgag atcacgcacc acgatgttgc cttggtaggg caaggtcagg
    18301 ttctcgatcg tgactttcat gatttcctct gctttctcag acggcggcgt ccgagtcgcc
    18361 gatcaggtcg ggatgactgt cttcggttat cgatgtgacg gtcggtgtga tcggcgtcac
    18421 catcagtatc gcgggcgtac ccgccggggt caggtggtgg cgggaatcac cgcgaaccgg
    18481 cgaggctggg ggttgattcg gcgatgaaca gttcggcggt cgcgatccgt cgcgccgcgc
    18541 gatgcaaagt gacggtcagg gggatgccgt ccgccgacgt tgtgatggtg gtgccgatga
    18601 caccggcggc agttccgatc cgcaattcac cgggcaaccc cgcccgggtc cgctcggcga
    18661 cggtggtgtg tggcactgca gctgccgctg ccacggcaac cgccgacgtc aggccgatcg
    18721 cggggtgcgg ggccagcatc gacaccatgc gcacggacac gtcgtagtcc tcggcaccga
    18781 caaccgcgcc tgtcgatgtt cggtattccg ttggcgcgcc gacaattccg actttgggca
    18841 ccgcatggac gacagggtcc tcgggtcgcg acaatcccat tgccagggcc gcttgccgac
    18901 gcagttcgac aagcaccggc agccgttcgg ttatcgcctc gttgccttcg gcaccggtca
    18961 tgccgaaatc ttcggcatag aaaagcgccg ccggcgcgcc tgcggcgacg agagttccgc
    19021 ggtactcccg gccttgcgcg accagcagat cctgggtgtt gccggtgggc aacagcaggg
    19081 cggacgagga cactactccg gcgaacgtca acgacaccgg cacgccaagc gcgctcgtac
    19141 cggggaccgc agcgatgccg tcggtaggga tgctggcgtt cggggtgtct atctcggccg
    19201 cgagcaccga gccggtgttt tggttgcgca tgcgcaccgt ggtacggccg cgatccacgg
    19261 ccaccatccc ggcttgcagg gcgtagagcg cgatcgccgt cgcgcagttt ccgcagttgc
    19321 tgccccactc gacctgccgc tggcccaccg cgacctgggc gaatagatag tcgacgtcga
    19381 tgtcgggctc ggcggaccgg cgcacgatcg cggccttgga cgtggtggaa ctgcccccgc
    19441 cgacgccgtt gagctgcagc ggatcgcccg aaccgaacgc cgaggtcaac agcccgtcga
    19501 ggtcgccggt ccgctgcacg agcggatcca cgtcgacggc gttgaacaac cagcacttac
    19561 tggtaccgcc acgcatccag gtgccacgca gtatgagcac gcacgcactc ctttcggtat
    19621 ctgtccgccc cgttcgctgc cgttgtgctc tcggtcacga cgggcgatgt cttcgagcgt
    19681 gcatcacaca agcctctaga acaatctcga aaatctcgac gtccattaag ttttgcttta
    19741 atctcttggc atgcactcgc ttgatccgcg cagggtgctg ctgctcgtcg acgtcgttcg
    19801 agccggctct ctgacagcgg cggccgcccg gctcgactac accacctcgg ctatctccca
    19861 gcagatcgcg aagttggaag ccgaagcggg acaaccactt ctggaacgcc acgcgcgtgg
    19921 tgtgcggctg accgaagccg gttcggcgct ggtgcgacgg gccgaaaaga tcgagacgca
    19981 cctgcaggcg gcgcgcaacg agctcgacga catcgccgga ttacgttcgg ggaccgtccg
    20041 aatcggcacg tttccgacgg cgggagccag cctgctgccg cgagtggtca agctcttcaa
    20101 gtcccgccac ccggccgtcg ccctgaccgt gcgcagttcc cgattcgcgg cgctgcgcgc
    20161 gatgctcgac tcccgcgaga ttgaactgtc actgttgtgg gactacgagt ggaatccagt
    20221 cactgacccc gaactgacgg tccgtcaact gctgatcgac ccgcccgcgc tgctggtgtc
    20281 ggcgaatcac cgcttcgcga aacgacgctc gatcaacatg accgagctgg ccggcgagca
    20341 gtggatcacc cgcgaggaca gccatccggt cggagccgcg ctcgaacgag cttgcagcgc
    20401 agcaggattc agtccaaccg tggcgttcgc tgccaacgat taccaagaag cccaggcgat
    20461 ggtggccgtc gatctcgggg tgtcgatggc accacgactg gccctatcca acttgcgcga
    20521 cgacgtccgc gtcatcccac tacggggggc gccctcccgc aggatcctgc tagtgcacct
    20581 ggccgagcac cgactgacac ccgcagaagc acaactatcc ggcatctttg ccgacgtcgc
    20641 gcgcaactgg cgcaggtagg cagacgtcac cagccagtcg tgaggacccg ttcgagggag
    20701 tcggcgaagt agtccaccga ttcgcgcgag acgcacaggg gcggcttgat tttgagcacg
    20761 ttctggaagt cgccggtggg ctgcatgatg acccccagtt cgagtagacg gtcgcagatc
    20821 gcggctgtct cttcggtggc cggctcgagg gtggcgcggt cacgaacgaa ctccaggccc
    20881 aggtacagac cggagccgtg aacggcgccc acgatcggat gcatctcggc gagcgcgaaa
    20941 aggcgatcct tcagatgcgc cccgacgatg cgcacgtttt cctgcagacc ctcctgctgg
    21001 atgacgtcga gcacggcgat gccgatcgca gacgagaccg gtgagccgcc agtggaggag
    21061 aagaagtacc cctgtgtgcg gtagcgctcg gctcgcttca tcactggaca gctaatcgcc
    21121 gtcgacggcg gcctagtgat gctgggcgcc tagattcgcg ttgactctgc gctaagggcg
    21181 acaaagtgcg agtaggcgac gccgtgtgcg cagagtcaac ggcaacggga tccgctaacg
    21241 cgacgcttcg tagcctgcgg tcaggtagtg caccgtgcgg tcgaggctgg gcaggatcgc
    21301 ggtgatgtcg atctcgccgg cggcgaagcg acccatcagg ccgtacacag cgctggagac
    21361 gatgtcgtcg acgtccttga cgaatgcggg gtcggcgtcg gcaagcacct ccatcgccgc
    21421 gggcacgacc gcgtcgaagc cacgccgaac cagcttctgg ccgccggggg ccgagcgggc
    21481 gcggaagtac gcgttgagca tcgcggggtg acgctcccac ggctcgaaga tggtgcgcag
    21541 cacgcgcatc aatccggcat acagcgactc gtcggcgtca tggggcttgg tcaatccgga
    21601 gtaccggttc tcttccatcc aggagttgag cgcggccaag atcaactcgt cgcgcgtgcc
    21661 gtagcgctta tagatggtgg ccagtgaggt gcgggcgcga cgggcgacct cgcgcagttg
    21721 cacggcgtcg tagccctcgg tttcgagcag ctcgacgacg atgtcgagga tccggtcctg
    21781 acccttgccg tccatgagta accaggttac cgtatcgagg gtaacgcggt tactcaacgc
    21841 aacgaagcga ggatcgatgg gttctttgga cggcaaggtc gccttcatca cgggcgtcgc
    21901 ccgcgggcag ggcagaagcc acgcggtgcg gctggccgcc gacggcgccg ccatcatcgg
    21961 cgtggacatc tgcgctgaca tcgcatcaaa cggatacccg atggcgtccc gcgaagagct
    22021 cgacgagacg gtggcattgg tggaggccgc gggcggcaag atggtcgcct cggtcgccga
    22081 cgtccgtgac tttcacgcgc tgaaggcggc gctggatgct ggggtggaac agttcggccg
    22141 gctggacatc gtgttggcca acgcgggcat tgcgacgatg gcgtttcgcg aggtgaccga
    22201 cgacgaggat ctcgaaatgt ggacggacgt cctcaacgtg aacctcgtcg gcgcctacca
    22261 caccgcgaag gccgcgatac cgcacctgat cgccggggga cacggcggtg caatcgtctt
    22321 caccagctct accgccgggt tgcggggttt cggcgggctg cagggcggcg gcttgggcta
    22381 cgcggcctcc aagcacggca tcgtcgggct gatgcgcacg ctcgccaatg cgcttgcacc
    22441 gcaaagcatt cgggtgaata ccgtgcatcc gacagccgtt aacaccatga tggccgtcaa
    22501 tccggccatg gtctcgttcc tggaaaacta tcctgacggc ggtccgcatc ttcagaatcc
    22561 gatgccggtc gaactgctgg agcccgagga catcagcgcc gcgatcgcgt acctcgtctc
    22621 cgatgcggcc aagtacgtca ccggtgtgac cttccccgtc gacgccggct tctgcaacaa
    22681 gttatgagcg gccgggttga aggcaagcgc gtcctaatca ccggggcggc aaggggaatg
    22741 ggccgcagcc acgctgtgcg cctggctgag gagggtgcgg acctgatcct cgtcgacatt
    22801 tgcgagtcga tacctgagtt ggaatacccg ctgtcgacgc gcgaagatct ggacgagacg
    22861 gcgcggctgg ctgaaaagca cggtggccgg gttgtcacag gcgttgtgga cgtgcgggac
    22921 gcggacgcgt tggccgcggt cgtcaacgac ggtgtcgccg agttgggcgg gctggacgct
    22981 gcggtcgcca acgcaggcgt gctcacggtc ggtacctggg atcagaccac acagcagcac
    23041 tggcgaacgg tgttggatgt caacctcatc ggcacgtgga acacctgcgc ggcggcgctg
    23101 ccgcatctgg tcgaccgcgg cggcagcctg gtcaacatca gctcggcggc agggttgaag
    23161 ggcaccccat tgcatctgcc gtacacggcg tccaagcacg gcgtggtcgg cctcagccgc
    23221 gcattggcca acgagcttgc cgcgcagaac attcgggtca acaccgtcca cccgacaggt
    23281 gtggaaactg gtatgcggcc ggagaacctg caacagatcc tcggcgaagg gcgcccggat
    23341 ctgatcccga tcttcctcaa cgccctgccc gccgtcatgg cagaagcgat cgatatcagc
    23401 aacgccgtgc tgttcctggt gtccgacgag tcgcgcatgg tgaccgggtt ggagttcaag
    23461 gtcgacgcgg gagtgaccat tcgatgacaa ctgttgatat cgaccgctcg gagcgtggcc
    23521 gacgcgagtt cgcggaagtc atgaccttcg atcccccaga cgattccagt ccgtcgacga
    23581 cgaacggctt gatcgacttc gtcttcgggg aagtctggtc gcggcccgga ttgagcagac
    23641 ggaatcggcg gttcatcacg cttccctgcg tggcggcggc cgatgccgaa ggccctctca
    23701 aggagcatgt gtatgccgcc ttgaacagcg gagatctgtc gatcaccgaa atgcgggaaa
    23761 cggtactgca tttcgcggtc tacagcggct ggccgaaggc gtcgcgcttc aacatggtgg
    23821 tcgacgagca atgggagcga atccaccgcg agcgcgggca caccgtgccg ccacccgatc
    23881 cgctgctacc gctggttacg ccgagcgacc cagaggaacg cctgctgacc ggagaacaat
    23941 cgttcaagga catcaactgc ctaccgttcg cgccgcaacg cgataacccg tatcaagggg
    24001 caggcattct gaacttcgtg ttcggcgaga tgtggctgcg gccgggattg ggcatgaagg
    24061 aacggcgact ggtgacagta gcgtgtgtgg ccttccagga cgcgccgtat ccgatcctga
    24121 gccacgtcta cgccgcgctg aagagccgag acgtgtcctt cgatgaaatg gatgagctgg
    24181 ccttgcattt cggcgcctac tacggctggg cgaaggcgtc gcatctgaat caggtgatcg
    24241 gcgagcagaa gcaacgcgtg ctcgacgagt ggcgccagga ggcgcagtga cagcgacatt
    24301 ggccggggga ctgctcatcg gcggtgaccg actcaccgaa acgtccggcg ggacatacgc
    24361 ccacatctac ccggcgacag gccaaccgaa cgcgcacatc ccgctggcag gggccgacga
    24421 gatcgaccgt gcggtcgcgt ctgcgtggga cgcgcaccgc gaatggatgg cgttgaccgt
    24481 cgaccggcgc cgcgatctgc tcatcgacct cgcggatgcg gtgcacgaga accttgacga
    24541 attgtcacgc ctcaacgtgc atgactacgc ggtgccaatc tccttcgcaa gcaacgcgat
    24601 tatgctggag cggttcttgc gtcacttcgc cggttacgtc gacaagccgc acgggagctc
    24661 gacgccggtc actggctcgt tcgacgtcaa cctgatcgaa cgcgagccgt acggtgtcgt
    24721 cggcgtcctc accccgtgga acggatcgct ggtggtgacc ggatcgtgcg tcgcgccggc
    24781 gctggcggcg ggaaatgcgg tagtgctgaa gccatcagag gtcgcgccgc tcgccgcatt
    24841 gcgcttcggg gaattgtgcc tcgaggttgg gctgcctgcc ggactggtca atgtcgtccc
    24901 cgccggaccg gagggcggcg acgccttggt gcggcatccg ggcatccgca agatccactt
    24961 cactggcgga gatgcgaccg cacgcaacgt tcttcaatcc gcggcggtca atctgacgcc
    25021 ggtggtgacc gaactgggcg gcaagtcggc gaacatcgtc ttcgacgacg ccgacctcga
    25081 cgccgcggcg atgctgtcgg cacaccaggg tccactcatg caatcaggac agagctgcgc
    25141 atgcgccagt cgcgtgctgg ttcaggagtc ggcgtacgac gcgttcctcg aaaagttcgt
    25201 ggctgtcatc gaaggctcga cggtcggcga cccgctcaac ccggaggtcg tattcggccc
    25261 ggtggtcagc cagcccgcgg ccgaccgcat cctcggcgtg atcgagcagg cagtcagcga
    25321 gcgttcgggg gagttgctcg tcggaggcac gcgcctcggc ggtgaactcg ccgacggcta
    25381 ctacattcag ccgaccgtgt tcggtgatgt cgacaaccgc tccgcgctgg cccaaaacga
    25441 gacgttcggg ccggtcgtat cggtgatgaa gttcagcgac gaggccgagg cggtgcgcat
    25501 cgccaacgac tcccgatatg gcttgaacgc atttgtccag accaacgatc tgcgccgggc
    25561 gcaccgcgtc gcgcgccagc tggaggctgg ttcggtgtgg gtcaacacgt tcagcgacat
    25621 ctccccgcaa ggaccgtacg gcggctataa gcagagcgga ttcggccgta ccggcggcct
    25681 tgaagggctg cacgaattcc tccaagtgaa gaacattcgc atcggcatgc agtgaggacg
    25741 agcagtccgg cacgaatttg tgcaagtgca cagcatcgcc atacgcgccg ggtgaagatg
    25801 atgggcgacg tcatcgagga gggtttatgg ccgggcgggt agaaggcaag gtcgcattca
    25861 tcaccggtgc cgcgcgcggc cagggccgga gtcacgcggt acggttggcg caggaaggcg
    25921 ccaacatcat cgccgtcgac atctgccagc ctttcgccga ctcgccggcc ccagcggcga
    25981 cccctgaaga cctcgcggag acagcggacc tggtcaaggc gctggacagg cgaatcgtga
    26041 ccgccgaggt cgacgtccgg gattacgacg cgctcaaggc cgcggtcgac agcggcgtcg
    26101 agcaactcgg cgggctagac atcatcgtcg ccaacgcagg catcggcaca gtcggaacac
    26161 ggctcgagcg gatgaaggaa gacatctggc aggagatgat cgacgtcaac ctcaccgggg
    26221 tgtggaagtc ggtgaaggct ggtgtcccgc acatgttggc aggcggacgc ggtggctcga
    26281 tcatcttgac cagttcggtc gcgggcctga agacataccc gcacaccggc cactacgtcg
    26341 cagccaaaca tggcgtggtc ggtttgatgc ggacattcgc cgttgagctt ggcgcgcagt
    26401 cgatccgggt caactcggtg catccgaccc atgtgaacac gccattgctg cacaacgagc
    26461 agacgtaccg gatgttccgt cccgatctgg aaaacccggg ccccgacgat atggccccgg
    26521 tctgtcagag cttccatatg ctgcccatcc cgtgggtgac cgccgaagac atcagcaacg
    26581 cggtgttgtt cctggcctcc gacgaggccc ggtacatcac aggcgttgcg ctaccggttg
    26641 acgctggcag ttgcctgaag tagcgcgaaa ggaccacccc ggcatgacaa ttgcgcacga
    26701 caccgacgtc tattacgacc cctacgacat cgacatcgtc aatgatccgt acccggtcta
    26761 tgcccggctg cgggaggaag cgccgatcta ttacaacgag cgcttcaact tctgggcgat
    26821 ctcgcggtac gccgacgtgg agaaggcgct ggccgattgg gagaccttct ccaacagccg
    26881 cggcgacatc ttggagctga tccagtcgaa cttcgacatg cccaagggcg tgatgatgat
    26941 ggaggatccg cccgtgcaca cgatgctgcg cgggctgatg tctcgggtgt tcacgccgcg
    27001 gcggatggcc gagatcgaag accagatccg gcggtattgc gtcaactgcc ttgacccgca
    27061 tgtgggctcg gatggcttcg acatcatcgc ggagcttgcg tcgatgatgc cgatgcgcgt
    27121 gatcggcatg ttgttgggta tccctgagtc ggagcagatc aacgtccgcg acgccaatga
    27181 cgcgaacctg cgcactaagc cgggcgcacc gatgaaggtc gtcaccgccg acaagatcgc
    27241 ggacggccgc atctacgcgg attacatcga ttggcgtgca aagaatccct ccgacgattt
    27301 gatgacggcc ttgctcaacg tcgagttcga agacgagaac ggcgtgacgc gcaagctcac
    27361 ccgcgacgag atcctgcgct acacccaggt ggtcgccggt gccgggaacg agacgaccgg
    27421 acggctgatc ggctggctgg ccaaggtgct tgccgagcat cccgatcagc gccgtgagat
    27481 cgagcaggac cggtcgctgc tgaaccgcgc cgttgacgag acgctgcggt tcgagccgac
    27541 cggtccgcac gtagcgcggt ggatggcaag ggatttcgag tgctacggca ccaccgtgcc
    27601 ggccggcagc gcgatgctgc tgttgttcgg agcggctaat cgtgactcgc gccgctacaa
    27661 ggacgccgag acgttcaaca tccaccgaga caacatctcg cacctgacct ttggcaaggg
    27721 tgtgcactac tgcctcggcg cgaaccttgc gcggctggaa ggccgcgttg ctctcgatga
    27781 actactcaac cggtggcccg aatgggacat cgactacgag acagcgcaat tggcgccgac
    27841 gtcgaccgtg cgcggctggg aacggctgcg gctggtcctg ccctgaggca cccgctattc
    27901 gacgaatgcg ccggcggcac acgttcaagc gatttcggcg gcggcgggtg gtgccggtgt
    27961 ggccagcccg tcgaatcgct gtatcctgcg gcgaagtcga gtcgtcggat cgaccgtcac
    28021 caacgacgcg agttcgcggc ggatggctgc ggcaacacgg cgagagaact gctccggttc
    28081 cgctcgtgca tcgggatatt caggaatgac gctgtcgagc gcaccgctgg ccagcaagtc
    28141 ggccgcgcga acgccttgcc gggccgctac ttctggagca cggtcggtgc tgtgatgcag
    28201 aatcgcgctg gcgccctcgg gtggcagcgg cgataaccat ccgtttgccg cgcccatcgt
    28261 gcgatcggcc ggaaacagcg ccagcgccgc cccgcccgtg ccctggccca gcagcagcga
    28321 aacagtgggc acctcgaggg cgagcatgtc ggcgatacaa cgggcgatct cgcctgcgat
    28381 cccgccttct tctgcagccc tggataattc gccacccggt gtgtcgatga cggacaccag
    28441 cggtaacccc aagtcgacgg ccacccgcat tccgcgccgc gcctgacgta acgccgcggg
    28501 accgatcggt gcgttcgccg gtgcgcggcg gtcctggccc aacaggacgc atgacacatc
    28561 ggaaaatcgg accagtgcga cgatcagcgc gggatcggct tccccttggc cggttccgcg
    28621 aagcggaacg acattcgtgg cggtggaaag tatctcctgg acgcctgggc ggtccggttg
    28681 tcgcgatgct acgaccgatg tccaggcagg aaaactgcgg gcgcctcggt agcagatgga
    28741 agcgacgccg ccaccattcg acgatcctga gacgatgttc agtgtccggg ccacgacttc
    28801 ggcgagctcg ttgggcgggc acaccgcgtc gacaagtcca cagcggaaca ggttttccgc
    28861 tgtttgcacg ccggtcggga atggatcgcc gtagagcgct tcgtacacgc gaggaccgag
    28921 aaaaccaacc aatgcgccgg gttcggcgaa ggtgacgtgt ccaagcgaac cccaggaggc
    28981 aaacacgccg ccggtggtcg ggtgccgcaa gtagacgaga tacggcaggc gagctgcctt
    29041 gtggtcggtc acggccgccg tgatccgcac catctgcagg aacgctgtcg tgccctcctg
    29101 catgcgcgtg ccgcccgacg ttggcagggc aagtagaggc aggcgttcgg cggtggcgcg
    29161 gcggatcgcc gtggtgagtc gatgaccggc ggccacgccg attgagccgc cgagaaatcc
    29221 gaactcgcac accaacatcg ccactcggtg gcctcggatg gtcgcggagc cggtgagcac
    29281 cgattcgtcg agcccggtct ttcttcgggc acaagccagc tcgtcaccgt actcggcgtc
    29341 cgccggcgtt tctcgaaggg cgccatccca cgagatccat gagcccgcgt cgatcagggc
    29401 atctatcacc tcacgggcgc tgagccgagt catcggtggt ccgccaggat ggtcgcttcg
    29461 atgagatcgt tgtcggcgcc aagcactggg ggagcggtgt ggtgcttgcg tgtcagttcc
    29521 gcgccctcgg cgtcgaagaa ccgcagcggc ggcccaggca gcgtgatgct tcccaacgtt
    29581 gcgtgatcga cgtcgatgag tagcccctgc gatttggtct gttcccattc gtagacctcg
    29641 cgaatggtgc gaatgcgccc ggcgggcaca ccgattcgat ccagttttgc gagcaactcg
    29701 tcggtgttgt agaacgcgaa ccgtgcctgc accatctctc tgacccgctt gctgttggcc
    29761 acccgctccg ggtttgttgc catccctgca gcgtcggcat cgaagccgaa tccttcgcag
    29821 agccgtttcc agagtccttc gctgccgacc gaaatctgca cggcgccatc ggccgtgtcg
    29881 aacagaccat atgggcaaat cgatggatgg tggttgccgg tggcctcgcc gacctggcct
    29941 gcgaccgtcc acttggtgcc ttgaaaggcg tgcactccga cgaccgcggc gagcagcgac
    30001 gcccgcacta cttggccccg gccggtgcgt tcccgttcct gtagtgccgc aaggactccg
    30061 aacgcgccgt acatcccggc cagcagatcg gcgatcggca ctccgacgcg ctgcacgtcg
    30121 gtggggtcgg aaccggtcac cgacatcagg ccggcttcgc cctgcgcgat ttggtcgtag
    30181 cccgcgcgcc cggcctcggg gccgtcgtgg ccgaatccac tgatcgacaa gacgaccagg
    30241 cgcggattga tctcggcgag ctgctgctgg gaaaacccaa ggcggtcaag gactccgggg
    30301 cggaagttct ccatcagcac atcggcccgg cggacgagtg cccgcagagc ggcgcggccg
    30361 gtttcctctt tgaggtccag cgtcactgac ttcttgttcc ggtttgcgca caagaagtac
    30421 gtcgactctc gctcgccgtg cgccgggtcg acgaacggcg gtccccagcc gcgggtgtcg
    30481 tcgccaccga ttgggttctc gaccttgacc acttcggcgc ctagatcgcc gagcatcatc
    30541 gccgcgtgcg gccccgccag ggcgcgggtg aggtcgacga cgacgagccc ggcgaggggg
    30601 ccggcgggtc gcgtcgagga ggggattgtc gatgtcatgg aggaccacct tcggcctagt
    30661 gactcagtga gctagtgg
//
LOCUS       DASRJA010000150         4640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2423718,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000150 DASRJA010000000
VERSION     DASRJA010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2423718"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            831..2124
                     /locus_tag="VFC01_07150"
                     /pseudo
     CDS             831..2124
                     /locus_tag="VFC01_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302603.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
     gene            2138..2917
                     /locus_tag="VFC01_07155"
     CDS             2138..2917
                     /locus_tag="VFC01_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015287862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN79450.1"
                     /translation="MPKKTEYEQGTPSWVDLQTTDQAAGKEFYASLLGWSYDDRPMPQ
                     GGTYSMALLNGDTVAAIAPMPPGAPEGRPPTWNTYIAVDDIAATIDKVGPAGGQVLMP
                     AMAVGDAGTMAFVADPTGAVVGLWQADQHIGATLVNEPGALIWNELITDKPESALAFY
                     EAVLGVSTGSMEMGPGQTYHLLKVGGTDVGGCMEPPMPEVPNHWHVYFAVPDADATAS
                     QAGSAGGQVAVEPFDIPNVGRSAVLMDPQGAMFSVLTPAAQ"
     gene            3033..4433
                     /locus_tag="VFC01_07160"
     CDS             3033..4433
                     /locus_tag="VFC01_07160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZN79451.1"
                     /translation="MERLSGLDASFLYIESPTQPLHVCSILELDTSTVPGGYTFEHLR
                     DELALRIAAIPEFRAKLADSQFNLDYPVWVEDDAFDLDRHLLRIDLPSPGGRRELAEI
                     CGHIATAELERDRPLWEMWVIEGVCDTDPCAGGLLAVMTKVHHAAVDGVTGASLLAQL
                     CSIEPDVPAPEPVDGGGGAGPLQIAAGGLLRFATRPWQLANVLPTTVATIAKTLRRAR
                     GGLTMAAPFAGPPTRFNALVTADRNVALAQLDLDDIKKVKDRFNVTVNDVVMALCASV
                     LRWFLSDYGELPGRSLVAMVPVSVHEKSDRPGHNQLSGMFCSLETHIGDPADRLRAIA
                     RADAAAKSHSSVISPTLLQDWAQLAARAVFGGVFRLVADSPLIGHPVHNLIVSNVPGP
                     QAQLYFLGCEVEAMYPLGPLFHGCGLNVTAMSLNGKLNVGVISCPDLLPDLWRLVDDF
                     DVALEELLECAGAVRI"
BASE COUNT          797 a         1423 c         1650 g          770 t
ORIGIN      
        1 gccagctcac ccctgtcaat cacgaccagg ccaagcacga gcgttccgat tccccgcggt
       61 ccgaaccacc cgacgaacag catggttcgt gccgaccggc cgctgcgcgc caacgcgacg
      121 gcgaccgcgg ccacccgcac cacgaccagc actccgacgg cgaaaagcac cacgcgccaa
      181 ccccttcgcg ccatgccggg atgaccgagt aggcaccgag gattgcgaac acgagaagtt
      241 ccaacacttg ggccgtcgcg tcggacactt gcgtgcgtgc cgtccggtca cctgcggtgg
      301 agcgggcaac catcgcgaac acaagaccgg ctacgaacgc ggtcacgaaa ccgctggtat
      361 gcaggcgttc accagtttcg aagctgatca gtgccagcgc cagcgttgcc aactgccccc
      421 atgtgtcatt catccagccg cgcctactcg accgtgaaat caccagcgcg ccgacaaggc
      481 cgatgacgac gcccgcagcc agggagacga gttcggtgcg gaatgcgaac cacgcccaac
      541 gcgtcggtgc gcgatgacga agagcaacgc gggagccaca caccagcgtt gaacgagacc
      601 cgagacgacc gcgtagccga gcactagacc agcgagggtc aacgcggctt cactgtgcac
      661 gggctcggta ccccctcacg cgtacgcgtc gattcatgag tccgttcggc aagccaggat
      721 tgcacggttc gacctgcgag gtgtgccgac agctgatcga gaggcggcac ggaccatcca
      781 accgttgacc aatacgttag cggcggctaa cgtatcgacg tgtgcctgac atgctggagg
      841 tcgcggcgga gccgacgcga cgccggctgc tgcagcttct tgcagcgggc gagctcaccg
      901 tcacccaact cgcatcgcaa ttttcggtca cccgctcggc gatatcgcag catctgggga
      961 tcctcgccga cgtcggtctt gtcaccgccc gcaagcaggg gcgggagagg tattaccggc
     1021 tcgacgagcg cggggtgcag cagcttcgcg cgctgttcga ttcgttctgg agcgacgagc
     1081 tcgaccgcct cgtcgccgac gccacacatc ccaaaaggag actccgccat gatatttgac
     1141 aaatctgtgg ttgtgccgct ggacccggag gcgacgttcg ctctcatcac ccagcccgac
     1201 aggcttcggc gatggatggc cgtcgctgcc cgcgtggagc tacggccagg gggtgactat
     1261 cggtggacgg tgacgccggg gcacaccgcg gcgggcacgt tcgtcgacgt ggagcccggc
     1321 aagcgggtgg tgtacacgtg gggctgggaa gacggggacc ccgcaccggg cgggtcgacg
     1381 gtgacagtca cgttgacgcc ggtcgacgcg ggcaccgagg tgcggctggt gcacgacggg
     1441 ctgaccgacg agcaggccgc acaccacgcg gtggggtgga accacttcat ggacagactg
     1501 gtggccgcag gccgggacgg cgacgcggga cccgacgaat gggcggcggc accggacccg
     1561 atcgacgaat tgctctgcgc cgaagcgaca ttggcagtgc tggagggcgt gctgcgtgac
     1621 atggactcct cggacctgac caagcagaca gcgtgcaccg agtacaccgt cgcgcagctg
     1681 ggcgcccacg tcatgggctc gatcaagaca ctcggcggct gcgcaggtgc ccggatgccg
     1741 gagcgcgatg agaatgcgcc gctggagact caggtcgcgg atgcggccca ggcggcgctc
     1801 gaggcctggc gtcggcgggg gctggagggt acggtcgagt tgggtccggg cgaggtgccc
     1861 gcagccctgc cagtcaatat cctctcactc gaattcttgg ttcacgcttg ggatttcgcc
     1921 agagccaccg gccgagatct agtggtctcc gagccggtgg ccgactatgc gctggaactg
     1981 gcgggcaagg tcatcactcc tgaatcgcgt gggttcgcgg gcttcgccga agcggtcgag
     2041 acggggcccg acgcccgagt gctggatcgg ctcatcgcgt tcaccggccg gcaaccggcc
     2101 gttgcccaaa catccgccaa ctaacgaaag gtccgtcatg ccaaagaaaa ccgagtacga
     2161 gcagggcacg ccgagctggg tcgacctgca aaccaccgac caggccgccg gcaaggagtt
     2221 ctacgcgtcg ctgctgggct ggagctacga cgaccggccg atgccgcagg gcggcacgta
     2281 ttcgatggcg ctgctgaacg gcgacaccgt cgcggcgatc gcgcccatgc cgccgggcgc
     2341 gccggagggc aggccgccga cgtggaacac atacatcgcg gtcgacgaca tcgccgcgac
     2401 aatcgacaag gtcggcccgg ccggcggcca ggtgctgatg cctgcgatgg ccgtcggcga
     2461 cgcaggcacg atggccttcg tggccgatcc gaccggcgcg gtcgtggggc tgtggcaggc
     2521 cgatcagcac atcggcgcga cgctggtcaa cgaaccgggc gcgctgatct ggaatgaatt
     2581 gatcacggac aagccggagt cggcgctggc gttctatgag gcggtcctcg gcgtgagcac
     2641 cgggtccatg gagatgggtc cggggcagac gtaccacttg ctcaaggtgg gcgggacgga
     2701 cgtcggtggc tgcatggagc cgccgatgcc cgaggtgccg aatcactggc atgtgtactt
     2761 cgccgtgccg gacgccgacg ccacagcctc gcaggcaggc tctgcgggcg ggcaggtcgc
     2821 tgtcgagccg ttcgacatcc caaacgttgg acggtccgcg gtcctgatgg acccgcaggg
     2881 ggcgatgttc agtgtgctga cgccggcggc gcagtaaccg gaggtcagcg gtcggtttac
     2941 tggcatacgc gggtgcgaat gccagtcgtg gaacttatta tgtttcggca caatgggttt
     3001 cgatgcgtag tcaatgcgcc tagtgtcggt gtatggagcg gctgagcggg ctcgatgcga
     3061 gtttcctcta catcgagagc ccgacccagc cgctgcatgt gtgctcgatc cttgagttgg
     3121 acacgtctac ggtgcccggt ggctatacct ttgaacatct tcgcgacgag ctggccttaa
     3181 ggatcgcggc gatacccgag ttccgggcaa agctggccga cagccagttc aatctggact
     3241 atccggtgtg ggtcgaggac gatgcgttcg acctcgatcg tcatctgctg cgcatcgacc
     3301 tgccgtcgcc gggagggcga cgggaattgg ccgagatatg cggacacatc gccaccgcgg
     3361 agctggaacg cgacaggccg ctgtgggaga tgtgggtgat cgagggcgtt tgcgataccg
     3421 acccgtgcgc gggtggtttg ttggcggtga tgaccaaggt ccatcacgcc gcggtggacg
     3481 gggtgaccgg cgcgagcttg ctggcgcagc tgtgcagcat cgagcccgat gtgccggcgc
     3541 cggagccggt ggacggtggc ggtggcgccg gcccgctgca gatcgcggcg ggtggcctgc
     3601 tgaggttcgc cacgcgtccg tggcagttgg ccaatgtgtt gccgacgacg gttgcgacaa
     3661 tcgcgaagac gctgcggcgt gcgcgcggtg gcttgacgat ggccgcgccg ttcgccggcc
     3721 cgccgacgcg gttcaacgca ttagtcaccg ccgaccgcaa cgtcgcgctg gcgcagctgg
     3781 acctggacga catcaagaag gtcaaggatc gcttcaacgt cacggtcaat gacgtggtga
     3841 tggcgttgtg cgcgtccgtg ctgcgctggt tcctcagcga ttacggcgag ctgcccggtc
     3901 ggtcgctggt agcgatggtg ccggtgtcgg tgcatgagaa gtccgaccgc ccgggacaca
     3961 accagttgtc ggggatgttc tgcagcctcg agacgcacat cggcgatccc gcggaccgac
     4021 tacgggcgat cgcgcgggcg gatgcggccg ccaagagtca cagttcggtg atcagtccga
     4081 cgctgttgca ggactgggcg cagctcgccg cgcgggcggt gttcggcggc gtttttcggc
     4141 ttgtcgccga ctcgccgctg atcggacacc cggtgcataa cctgatcgtg tcgaatgtgc
     4201 cgggcccgca ggcgcagttg tacttcttgg gctgtgaagt tgaggcgatg tatccgctgg
     4261 gcccgctgtt ccacggctgc gggctgaacg tcacggcgat gtcgctgaac ggcaagttga
     4321 acgtcggcgt tatctcatgc ccggacttgc tgcccgacct gtggcgactg gtcgacgact
     4381 tcgatgttgc gctcgaggag ttgcttgaat gcgcgggggc ggtgcgcatt tgaagtcttg
     4441 gtggcgccgt cgaccaacga ctttggtgtc accgcggtgt gtggtgaagc tgttggggcg
     4501 gaggcggatc gagccattcg aggtgagccg gtgactaacg tcgtggcggg cgagcgggtc
     4561 gtcgatgtcg aggcgggagt cggtgaacga ggatttgagt agggcgcggg cggcgctgat
     4621 ggcgtcgttg agttggcggg
//
LOCUS       DASRJA010000151         6632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3360747,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000151 DASRJA010000000
VERSION     DASRJA010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3360747"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1021)
                     /locus_tag="VFC01_07165"
     CDS             complement(152..1021)
                     /locus_tag="VFC01_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZN79452.1"
                     /translation="MSQWTAADLPSFSGRTVIVTGANSGLGLVTARELARVGAKTILA
                     VRNTAKGDEAAATMTGDVEVRKLDLQDLASVRAFADGVDGVDVLINNAGIMAVPYAQT
                     VDGFESQIGTNHLGHFALTNLLLPKVTERVVTVSSQMHLMGWVNLNDLNWKSRPYFAW
                     PAYGQSKLANLLFTKELQRRLDAAGSRVKAHAAHPGYSATNLQSHSSGFFAKVFQAGN
                     NLVATDADFGARQTLYAVAQDLPGNSFIGPRFAMWGPTGPTFLRSPLARDAKKATALW
                     ELSEQLTDTKFPL"
     gene            complement(1041..1664)
                     /locus_tag="VFC01_07170"
     CDS             complement(1041..1664)
                     /locus_tag="VFC01_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqN/LpqT family lipoprotein"
                     /protein_id="HZN79453.1"
                     /translation="MFAATGCGAEQPDYHSLWTTPSPTPTPTTTEAPVPISKFLEESG
                     VAGESVAPDKLTDVTVTMPRPKGWQQYSNPNFAPGTRVIAKGDTYPTAMLLVFKLTGD
                     FDVNEAIKHGYADAELSQNFKRLNASTDNWHGFPSAMIEGSYDLNGRRMHSYNRIVIP
                     TGAPPANQRYLVQFTVTGYAEKAAEEAPDIESVIGGFDVKVPGAPPK"
     gene            complement(1697..2041)
                     /gene="arsC"
                     /locus_tag="VFC01_07175"
     CDS             complement(1697..2041)
                     /gene="arsC"
                     /locus_tag="VFC01_07175"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254208.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HZN79454.1"
                     /translation="MAKSVIYHNPKCSTSRKTLDLLRDNGIEPEIIQYLKTPPTRAEL
                     VNMIKDAGIDVSKAVRKREALYDELGLADASDDELLDAMAEHPILIERPFVVTPRGTR
                     LARPIDAVREIL"
     gene            complement(2041..3021)
                     /locus_tag="VFC01_07180"
     CDS             complement(2041..3021)
                     /locus_tag="VFC01_07180"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HZN79455.1"
                     /translation="MGTEWTDNRKNLMLFSGRAHPELAEQVAKELDVPVTAQTARDFA
                     NGEIFVRFDESVRGCDAFVLQSHPWPLNQWLMEQLIMIDALKRGSAKRITAILPFYPY
                     ARQDKKHRGREPISARLVADLLKTAGVDRIITVDLHTDQIQGFFDGPVDHMRAQNLLC
                     GHIAENYADHDMVVVSPDSGRVRVAEKWADALGGVPLAFIHKTRDPLVPNQVKSNRVV
                     GDVKGKTCILTDDMIDTGGTIAGAVKLLKEDGAEDVIIAATHGVLSDPAPQRLAECGA
                     REVIVTNTLPIGEDKQFPQLTVLSIAPLLASTIRAVFENGSVTGLFDGSA"
     gene            complement(3098..4570)
                     /gene="glmU"
                     /locus_tag="VFC01_07185"
     CDS             complement(3098..4570)
                     /gene="glmU"
                     /locus_tag="VFC01_07185"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZN79456.1"
                     /translation="MTTHGEAAVLVLAAGAGTRMRSDIPKVLHTLAGRSMLSHALRSV
                     AKATPGHLVVLLGHDRDRVTPAVAELADELGRPIQIAVQEEQLGTGHAVSCALSALPE
                     DYAGTVVVTYGDVPLLDADTIDGLIAAHSTGSAAATLLTTALPDPTGYGRVVRTPDDE
                     VLEIVEEADATPTQRAIQEINAGVYAFDAAELRSALSRLQPHNAQQELYLTDVVSILR
                     SDGYVVRARHVDDSTLVAGVNDRVQLAELGAELNRRIVAAHQRAGVTIIDPATSWVDV
                     DVTIGRDTVIHPGTQLLGSTRIGERCQIGPDTTLTDVTVGDEAAVVRTHGGQSAIGDG
                     AAVGPFAYLRPGTVLGAEGKLGAFVETKNATIGTGTKVPHLTYVGDADIGEHSNIGAS
                     SVFVNYDGETKSRTTIGSHVRTGSDTMFVAPVTVGDGAYTGAGTVIREDVPPGALAVS
                     SGPQRNIEGWVSRKRPGSEADKAARRATGEQENPDDAAKP"
     gene            complement(4588..4659)
                     /locus_tag="VFC01_07190"
     tRNA            complement(4588..4659)
                     /locus_tag="VFC01_07190"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4624..4626),aa:Gln,seq:ttg)
     gene            complement(4704..5186)
                     /locus_tag="VFC01_07195"
     CDS             complement(4704..5186)
                     /locus_tag="VFC01_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007531735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HZN79457.1"
                     /translation="MTRIHRNNNVVGWLSIALGLITSTTPVWAQTSPIGAGFTLGLGA
                     MTVMYGAWSLIARDPTRDHWAMSVVGLVLAIAPWIGGYAGDAAAWVSWIGGITLMVLA
                     GAAYIADEANEVTRTERIKALVNYRVRHDQATEGISDLSRPRELVDALPIRHRHGADY
                     "
     gene            5266..5835
                     /locus_tag="VFC01_07200"
     CDS             5266..5835
                     /locus_tag="VFC01_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010594962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79458.1"
                     /translation="MPVDPHRRDAMITAAVLDLLRTKGPKAVNVEAVAAHAGVAKTTI
                     YRRYRDREEMIAAALTSLTSPPPPPEQPTPEALLQWLIEQSVNAIEGGIGLGGIAALL
                     TDEDTTFTELIRALLVEHRAALARELHDAADPAELRDLDIETLLDCIVGAYLAERARR
                     GTVEPGWTQRVLHCLRPAFTEQPPKRRTS"
     gene            5906..6511
                     /locus_tag="VFC01_07205"
     CDS             5906..6511
                     /locus_tag="VFC01_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN79459.1"
                     /translation="MTGAERRHQLIDVARTLFAERGYEGTSIEEIAQRANVSKPVVYE
                     HFGGKEGLYAVVVDREMSALLDGITSSLTRMTNNRSRLRIERVALALLTYVDERTDGF
                     RILIRDSPAAITSGGTYSTLLNEAVNQVSSILAGDFSRRGLDPDMAPLYAQALVGSVS
                     MTAQWWLDVREPKKEVVAAHLVNLCWNGLMHLEFDPQLLDE"
BASE COUNT         1070 a         2141 c         2227 g         1194 t
ORIGIN      
        1 gaagtcttag ccattggtat tgctcctgtg ccggtcggtc gtttcgggca cggccagggt
       61 cgcaaaccat cagatgtgcg ggttcccgtc gataacggtg gccggagacc accctcgccg
      121 tgacgcccgg ccaggttagc gcatcagcag ctcagagcgg aaatttggtg tctgtgagct
      181 gctcagatag ctcccagagc gcggtcgctt tcttggcgtc gcgagccagc gggctgcgaa
      241 gaaaggtggg cccggtaggt ccccacatcg cgaaccgggg gccgatgaag ctattgcccg
      301 gcaggtcctg tgcgacggcg tacagcgtct ggcgggcgcc gaagtcggcg tcggtggcga
      361 caaggttgtt gccggcctgg aagaccttgg cgaagaaacc cgatgaatgc gactgcaggt
      421 tggtggccga gtacccaggg tgtgcggcgt gcgccttgac ccgagacccg gcggcgtcga
      481 ggcggcgctg cagctccttg gtgaacagca ggttggccag cttggactgc ccgtaggccg
      541 gccacgcgaa gtagggccgc gacttccagt tcaggtcatt taggttgacc caccccatca
      601 ggtgcatctg cgacgacacc gtcaccaccc gctcggtgac cttgggcagc agcaggttgg
      661 tcagcgcgaa gtggccgagg tggttggtgc cgatctggct ttcgaagccg tcgacggtct
      721 gtgcgtacgg caccgccatg atgccggcgt tgttgatcag cacgtcgacg ccgtcgacgc
      781 cgtcggcgaa ggcgcgcact gacgccaggt cctgcaggtc cagcttgcgg acctcgacgt
      841 caccggtcat ggtggccgcg gcttcgtcgc ccttggccgt attgcgcacg gccaaaatcg
      901 tcttggcccc gacgcgggcg agctcgcgcg cagtgaccag tcccagcccg ctgttcgcgc
      961 cggtgacgat gacggtgcgt ccggaaaacg acggcagatc cgcggcagtc cactgactca
     1021 tggcgcctac cctaatgcgg ttacttcggc ggcgcgcccg gcaccttgac gtcgaatcct
     1081 ccgatcaccg actcgatgtc gggcgcttcc tcggcggcct tctccgcgta acccgtcact
     1141 gtgaactgaa cgaggtagcg ctgattggcc ggcggcgcac ccgtcgggat gacgatgcgg
     1201 ttgtagctgt gcatgcggcg gccgttgagg tcgtagctgc cctcgatcat cgccgacggg
     1261 aagccatgcc agttgtcggt ggaggcgttg agccgcttga agttctgcga cagctccgcg
     1321 tcggcgtagc cgtgcttgat ggcctcgttg acgtcgaagt cgccggtgag cttgaacacc
     1381 aacaacatcg cggtggggta ggtgtcgccc ttggcgatca cccgcgtgcc cggcgcgaag
     1441 ttcggattgc tgtactgctg ccagcctttc ggccgcggca tcgtcaccgt cacatcggtg
     1501 agcttgtcgg gcgcgacgga ctcgccggcg acccccgact cctcgaggaa cttggagatc
     1561 ggcaccggcg cctcggtggt agtgggtgtg ggcgtcgggc tcggcgtcgt ccacagcgag
     1621 tgatagtcag gctgttccgc gccgcagccc gttgcggcga acatgacgag cgcagcggcg
     1681 gcccagtgcc gaaacgtcac aaaatctcgc gaaccgcgtc gatcgggcgg gccaaccggg
     1741 tgcccctggg tgtcacgacg aatgggcgct cgatcaggat cgggtgttcg gccatcgcgt
     1801 cgagaagttc gtcgtctgac gcgtcggcca agccgagctc gtcgtacagc gcttcgcgct
     1861 tgcgcacggc cttgctcacg tcgatccccg cgtccttgat catgttgacc agctcagcgc
     1921 gcgtcggcgg cgtcttcagg tactggatga tctccggttc gatgccgttg tcccgcaaca
     1981 gatccagggt cttacgcgac gtggaacatt tgggattgtg atagatgacg ctttttgcca
     2041 ctacgccgac ccgtcgaaaa gccctgtcac cgaaccattt tcgaacaccg cacggatggt
     2101 gctggccagc agcggcgcga tggacaagac ggtcagctgc gggaactgtt tgtcctcgcc
     2161 gatcggcagc gtgttggtga cgatcacctc acgggcgccg cattcggcca gccgctgcgg
     2221 agcgggatcg gacagcacac cgtgcgtggc ggcgatgatg acgtcctcag cgccgtcctc
     2281 cttgaggagc ttgaccgcac ctgcgatcgt gccaccggtg tcgatcatgt cgtcggtgag
     2341 gatgcacgtc ttgcccttca cgtcgccgac gacgcggttg gacttcacct ggttgggcac
     2401 cagcggatcg cgggtcttgt ggatgaaggc cagcggcacg ccaccgagcg cgtcggccca
     2461 tttctcggcc actcgcacgc gacctgaatc gggggagacc acgaccatgt cgtgatcggc
     2521 atagttctcg gcgatgtggc cacacaacag gttctgcgcg cgcatgtgat ccaccggccc
     2581 gtcgaagaag ccctggatct ggtcggtgtg cagatcgacg gtgatgatcc ggtccacgcc
     2641 ggccgtcttg agcaggtcgg caaccagccg cgccgagatc ggctcgcggc cgcggtgctt
     2701 cttgtcctga cgcgcgtagg gatagaacgg caggatcgcg gtgatccgct tggcgctgcc
     2761 gcgcttgagc gcatcgatca tgatcagctg ctccatcagc cactggttca gcggccatgg
     2821 atgcgactgc aggacaaacg cgtcgcagcc gcggaccgat tcatcgaagc ggacgaagat
     2881 ctcgccgttg gcgaaatcgc gtgcggtctg cgcggtgacc gggacgtcga gctccttggc
     2941 cacctgttcg gccagctccg ggtgcgcgcg gcccgagaag agcatcagat ttttgcggtt
     3001 gtcggtccac tcggtgccca ctggtcgccc tcgttgcttg gggatcgata cggcctaatc
     3061 gtacgggtag ccagaagccc aacaaccggc aaccggctca cggctttgcg gcgtcgtccg
     3121 ggttttcttg ttcgccggtg gcccgacggg cggctttatc ggcttccgac ccgggccgtt
     3181 tccgcgacac ccaaccctcg atgttgcgct gcgggcccga cgagacggcc agcgcaccgg
     3241 gcggcacgtc ctcacggatg acggtgcccg cgccggtgta tgcgccgtcg ccgacggtga
     3301 ccggggcgac gaacatggtg tcggatccgg tccgcacgtg cgagccgatc gtcgtccggc
     3361 ttttggtttc tccgtcgtag ttgacgaaca cgctggaggc gccgatgttg ctgtgctcgc
     3421 cgatgtcggc gtcgccgacg taggtcaggt gcggcacctt ggtgccggtg ccgatcgtgg
     3481 cgttcttggt ttcgacgaac gcgccgagct tgccttcggc gccgagcacg gtgccgggcc
     3541 gcagatacgc gaacggcccg acggccgcgc cgtcgccgat cgccgattga ccgccgtggg
     3601 tgcgcacaac tgcggcctcg tcgccgacgg tgacgtcggt gagtgtggtg tcggggccga
     3661 tctgacagcg ctcgccgatg cgagtcgagc cgagcagttg cgtgcccggg tggatcaccg
     3721 tgtctcgacc gatggtgacg tcgacgtcga cccagctggt ggccggatcg atgatcgtca
     3781 cgccggcccg ctgatgcgcg gcgacgatgc gccggttgag ctcggcgccc agctcggcca
     3841 actgcacccg gtcgttgacc ccggcgacca gcgtcgagtc gtcgacgtgt ctggcccgca
     3901 cgacgtaccc gtcggagcgc aggatcgaga ccacgtcggt caggtagagc tcctgctggg
     3961 cgttgtgggg ctgcagtcgg ctcagcgccg agcgcaattc tgcggcgtcg aaggcgtaga
     4021 caccggcgtt gatctcttga atggcccgct gggtcggggt ggcgtcggcc tcctcgacga
     4081 tttcgagcac ctcatcgtcg ggcgtgcgca cgacacgccc gtagccggtg ggatcgggca
     4141 gcgcggtggt gagcagggtc gcggccgccg acccggtgct gtgcgcggcg atgaggccgt
     4201 cgatcgtgtc ggcatccagc agcggcacat cgccataggt caccacgacg gtgccggcgt
     4261 agtcctcggg cagcgccgac agcgcgcagc tcaccgcgtg gcccgtgccg agctgctctt
     4321 cctggaccgc gatttggatg ggccggccca actcgtcggc gagctcggcg acagcgggcg
     4381 tgacccggtc gcggtcatgg cccagcagga ccacgagatg gcctggtgtt gccttggcca
     4441 ccgagcgcag cgcatgcgac agcatgctgc gaccggcgag ggtgtgcagc accttcggga
     4501 tatccgaccg catgcgggtg ccggccccgg cagctagcac caggaccgcg gcctcaccat
     4561 gggttgtcat cgagcctccg catagagctc cgtcgccagg actcgaacct gaactatctg
     4621 aaccaaaatc agaggtgctg ccgattacac cacgacggac tgatcaactc gtgatgctag
     4681 tcgaaccgac cctcagtcct gcgtcagtag tcggcgccgt ggcggtggcg gatgggcagc
     4741 gcatcgacga gctcacgcgg ccgactgaga tcgctgatgc cttccgtcgc ttggtcgtga
     4801 cgaacgcggt agttgacgag agccttgatg cgttccgttc gggtcacctc gtttgcctca
     4861 tcggcgatgt aggcggcgcc ggccagcacc atcagcgtga tgccgccgat ccacgacacc
     4921 cacgccgcgg cgtcgccggc gtatccgccg atccagggtg cgatggcaag aaccagcccg
     4981 acaacggaca tggcccagtg gtcgcgcgtg ggatcgcggg cgatgagtga ccaggcgccg
     5041 tacatgaccg tcatcgcacc cagacccagg gtgaagcccg ctccgatcgg tgaggtctgc
     5101 gcccagactg gggtggtcga cgtgatcagt ccgagtgcga tcgacagcca accgacgacg
     5161 ttgttgttcc gatgaatcct tgtcactttg tctccttacc tatacgactc ctatacaata
     5221 cgcaacgcgt atcgtttcgt ttaaattccc tgatcaagga gcgacatgcc agttgatccg
     5281 cacagacgcg acgcgatgat cacagcggcg gtcctcgacc tgcttcgcac caaagggccg
     5341 aaggcggtca atgtcgaggc ggtcgccgca catgccggcg tcgccaagac gacgatctat
     5401 cgccgctacc gcgatcgcga agagatgatc gctgcggcgc tcacttcgct gacaagtccg
     5461 ccgccgcccc ccgagcagcc gacgccggag gcgcttctcc agtggctcat cgagcagtcc
     5521 gtcaacgcga ttgaaggcgg catcggactg ggcggcattg cggctcttct caccgatgag
     5581 gacaccactt tcaccgaatt gatccgtgcg ctgctggtcg aacaccgcgc cgcgctggcg
     5641 cgggaactcc acgacgccgc cgaccccgcc gagcttcgcg acctcgacat cgagacgctg
     5701 ctcgactgca tcgtcggcgc ctacctcgcg gagcgcgcgc gccgcggaac tgtcgagccg
     5761 gggtggactc aacgcgtcct gcactgcttg cggcccgcgt tcacggagca accgccgaag
     5821 cggcgaacaa gttaggcgaa ttcagaggtt taccctgaga ggcgtggcag cacccgacaa
     5881 ggaagtgcgt gcaccccgcg cgaggatgac cggcgccgag cggcgtcatc agctgatcga
     5941 cgtcgcacgg acgttgttcg ccgaacgcgg gtatgaggga acttcgatcg aggagatcgc
     6001 gcagcgcgcc aacgtctcca aacccgtcgt ctatgaacac ttcggcggca aggaggggct
     6061 ctacgcggtt gtggtcgatc gcgagatgtc ggcgctgctc gacggcatca cgtcctcgct
     6121 gacccggatg accaacaatc ggtcccgtct gcgcatcgag cgggtcgcgc tggcgctgct
     6181 gacctatgtc gacgaacgca ccgacgggtt ccgcatcttg attcgtgact ccccggcggc
     6241 catcacgtcg ggcggcacct actccaccct gctcaacgag gccgtcaacc aggtgtcgtc
     6301 gatcctggcc ggtgacttct cccggcgcgg gctcgacccc gacatggcac cgctgtacgc
     6361 ccaggcgctg gtcgggtcgg tgtcgatgac ggcgcagtgg tggctcgacg tgcgcgagcc
     6421 gaagaaagaa gtggtggccg cgcatctggt caacctgtgc tggaacgggc tcatgcatct
     6481 cgagttcgac ccgcagctgc tcgacgagtg atttgcccgc tccgacaagc acttcggcgc
     6541 ccacctagga tggttgcatc atgaccgcat cggggaccgc tcgcgtccaa accccgatcg
     6601 cggggctcgt tgacttggcg ttgcgggatc ca
//
LOCUS       DASRJA010000152         6042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_452360,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000152 DASRJA010000000
VERSION     DASRJA010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452360"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..950
                     /locus_tag="VFC01_07210"
     CDS             24..950
                     /locus_tag="VFC01_07210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005100517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZN79460.1"
                     /translation="MSKAVQYDSYGDIDVLEVRDVPRPTPGTGEVLVEVKAAGINPGE
                     AMIRRGALHDRWPTTFPSGQGSDLAGVVAETGDGAETFAVGDEVLGFTDDRASHAQLV
                     VVPAGQLTAKPPEVPWDVAGALYVAGTTAYAAVRSVALRPGDTVAVAGAAGGVGTIAV
                     QLAKRAGATVLGIAGPSNDDWLTAHGVVPVNYGDGLANRLRDAAPDGRVDAFLDFFGG
                     GYVELAVSELGIDAQRVDTIIDFPAIERFGVKFEGNANAAEARVLAELADLIAAGELE
                     VPVARVYPLDQVKEAFRTLEQRHTRGKIVLRP"
     gene            complement(958..1626)
                     /gene="phoU"
                     /locus_tag="VFC01_07215"
     CDS             complement(958..1626)
                     /gene="phoU"
                     /locus_tag="VFC01_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZN79461.1"
                     /translation="MRTAYHEQLDALTAQLGEMCGLAGAAMERATQALLQADLVLAEQ
                     VITDHEQIAAMSAKAEESAFVLLALQAPVAGDLRAIVSSIQIVADVDRMGALALHVAK
                     IARRRHPQHALPEEVNGYFAEMGRVAVELGNSAQEVLITRDPEKAARIREEDDAMDDL
                     HRHLFTVLMDREWKHGVAAAVDVTLLGRFYERFADHAVEVARRVIFQVTGNYPDEEEI
                     PAPR"
     gene            complement(1731..3746)
                     /locus_tag="VFC01_07220"
     CDS             complement(1731..3746)
                     /locus_tag="VFC01_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZN79462.1"
                     /translation="MSDGDNATPGLPGAGDNPWLTRSARPSPGAAPWERNGHSDSGDI
                     NEEEHTGNHTDGVTVADLIAKVHGASSVPEELRRPRPEPEPPAPATEVIAAVRPERED
                     PNTEVIPVVAGYASEVPDLATLRQRDRVQPSRVGTERSTPAPKPRRRHLTKTVVAGRA
                     AAALIAVSALALTGGAWQWQSSKNNMLNKVSALDPDSRDILDPNAQFGDENFLIVGVD
                     SRFGENSDMGAGTTEDAGGARSDTIMLVNIPANRKRVVAVSFPRDLAITPTKCEHWDP
                     DTGQYGPVTDEQSEMYGQKEVYTETKINSAYAVGGPKCLVKVVQKLSGLSVNRFMAVD
                     FAGFSKMVDAIGGVEVCSTTPLEDYELGTVLANAGRQMVDGHTALQYVRARQVTTETN
                     GDYGRIKRQQLFLSSLLRSLISKEVFFSLSKLNNVVNMFIDDSFVDNIKTKDLVDLGQ
                     SIQGVNAGRITFVTVPTVGYADEYGNETPREGDMRALFDAIINDDPLPEEKNPDNTPV
                     PGTPESTTSAAPEAAPAPAQDNNAELVNAVTTNPQDITVRVSNSTGQDGLASTAATEL
                     QSHGFNVTSPDDYPGPLNSTTVFFSPGNEQAAATVASSFTNPTIERVSGLGDVVRVVL
                     GSDFYSVAPPSPSGSPVQVHVVHGTSTSPTHLPEDLTVTNAADTTCE"
     gene            complement(3944..5077)
                     /gene="dusB"
                     /locus_tag="VFC01_07225"
     CDS             complement(3944..5077)
                     /gene="dusB"
                     /locus_tag="VFC01_07225"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZN79463.1"
                     /translation="MRIGPIELRSPVVLAPMAGVTNVAFRTLCRELELARAGTVSGLY
                     VCEMVTARALVERHPVTMHMTTFAPDETPRSLQLYSVDPQNTYAAAKMIGDEGLADHI
                     DMNFGCPVPKVTRRGGGAALPFKRRLFGKIVAAAVRATEGTTIPVTVKFRIGIDDAHH
                     THLDAGKIAAEEGAAAVALHARTAAQRYSGTADWDQIALLKQHVTTVPVLGNGDIFDA
                     DDALAMMATTGCDGVVIGRGCLGRPWLFAELSAAFAGTTPAMPPTLGEVAAIMRRHGE
                     LLAAHFGEDKGMRDMRKHIAWYLHGFPAGADLRRALALVKTLAELESLLEQLDPAVPF
                     PQAATGPRGRQGSPASVALPEGWLADPDDCTVPAGADVMHSGG"
     gene            complement(5095..>6042)
                     /locus_tag="VFC01_07230"
     CDS             complement(5095..>6042)
                     /locus_tag="VFC01_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HZN79464.1"
                     /translation="EKNVNRHLTMRKDWNPHDYIPWSDGKNYYALGGKDWDPEESKLS
                     EVAQVAMLTNLLTEDNLPSYHREIAMNFGMDGPWGQWVNRWTAEENRHGIALRDYLVV
                     TRAIDPVELELLRVEQVTRGFSPGQNQQGELFAESLFDSVIYVTFQELATRVSHRNTG
                     KACQETIADQLLQRVSADENLHMIFYRDVSDAGFDIAPNQAMASLHRVLRNFKMPGYT
                     VPEFRRKAVIIAVGGVYDPRIHLDDVVMPVLKKWRIFEREDFTGEAAAMRDDLGELVK
                     ELEDTCEKFEVAKERRLERERKVAEKKAAKNLLVTSSAS"
BASE COUNT          927 a         1947 c         2041 g         1127 t
ORIGIN      
        1 cggctgatgc gttcgttcct cgcatgagca aggcagtgca gtacgacagc tacggcgaca
       61 tcgatgtgct cgaggttcgc gacgttccgc gaccgacacc cggcacgggt gaggtgctcg
      121 tcgaggtcaa ggcggcgggt atcaatccgg gcgaagcgat gatccgccgg ggcgccctgc
      181 acgatcgttg gccgaccacg tttccgtcgg gtcagggcag tgacttggcc ggcgttgtgg
      241 ccgaaaccgg cgacggtgca gagactttcg ccgtcggcga cgaggtgctg ggcttcaccg
      301 acgaccgcgc gagccacgcg cagttggtcg tcgtgccggc tgggcagctc accgccaaac
      361 cgccggaggt gccatgggac gtcgccggtg cgttgtatgt cgcgggcacc accgcatacg
      421 ccgcggttcg ctccgtcgcg ttgaggcccg gcgatacggt cgccgtcgcc ggcgcagcag
      481 gaggagtcgg caccatcgcc gtccaattgg ccaagcgcgc cggcgccact gtgttgggca
      541 tcgccggtcc gtcgaacgac gattggctca ccgcgcacgg cgtcgtcccg gtcaattacg
      601 gcgacggact ggcgaaccgg ctacgagacg ccgcgcccga tgggcgggtc gacgcgtttc
      661 tggacttttt cggcggcggc tacgtcgagc tggcggtgtc cgaactgggc atcgatgcgc
      721 agcgggtcga cacgatcatc gacttcccgg ccatcgagcg gttcggggtc aagttcgagg
      781 gcaatgccaa tgcggccgag gcccgggtgc tcgcggaatt ggctgacttg atcgccgcag
      841 gtgagctgga ggtcccggtg gcccgggtct acccgcttga ccaggtcaaa gaggcgttcc
      901 gcacgctgga gcagcggcac accaggggaa agatcgtcct gcgcccgtga agggacgtca
      961 gcgtggggcg gggatctctt cctcgtccgg ataattgccg gtgacctgga agatgacgcg
     1021 gcgtgcgacc tcgacggcgt ggtcggcgaa tcgctcgtag aaccggccga gcagggtcac
     1081 gtcgacggcc gcggccaccc cgtgcttcca ttcccgatcc atcagcaccg tgaacagatg
     1141 acggtgcagg tcatccatcg cgtcgtcttc ttcacggatc cgggctgcct tctccggatc
     1201 gcgggtgatc agcacctctt gggcactgtt gcccaattcg actgcgacgc ggcccatttc
     1261 ggcgaagtag ccgttgacct cttcgggcag cgcgtgctgt gggtggcgac gccgggcgat
     1321 cttggcgacg tgcagcgcaa gtgcgcccat ccggtcgaca tcggcgacga tctggatcga
     1381 gctgacgatc gcccggagat caccggcgac cggcgcctgc agtgctagca gcacaaaggc
     1441 gctctcctcc gctttggcgc tcatcgcggc gatctgctcg tggtcggtga tgacctgttc
     1501 ggccagcacc agatccgcct gcagcagggc ctgcgtcgcg cgttccatgg cagcgcccgc
     1561 cagcccgcac atctcgccga gctgggctgt taaggcatcc aattgctcgt ggtacgcggt
     1621 tcgcatggac caagcctacg gggtatctcg gcgaaacgtc acgacgcgcg cggtgaacga
     1681 aaggtgaatg ccacctgcct aaacgtcatc ggcagcggcg gccttgcggc ttattcgcac
     1741 gtggtatcgg ccgcgttcgt gaccgtcaga tcctcgggta ggtgtgtcgg cgaggtgctg
     1801 gtgccgtgaa cgacgtggac ctgcacgggg gagccgctgg gcgacggggg cgccaccgag
     1861 tagaagtcgc ttcccagcac cactcgcacc acatcgccaa ggccggagac ccgttcgatg
     1921 gtggggttgg tgaacgacga ggcgaccgtc gccgcggctt gttcattgcc cggcgagaag
     1981 aagaccgtcg tcgaattcag cggtcccggg tagtcgtcgg gcgaggtcac attgaagcca
     2041 tgcgactgca gttcggtggc cgcagtggac gcaagcccat cctggccggt cgaattcgag
     2101 acgcgcaccg tgatgtcctg cgggttggtg gtgaccgcgt tcaccaactc ggcgttgttg
     2161 tcctgcgccg gtgccggagc agcctccggc gccgcgctcg tcgtcgactc cggagtgccg
     2221 ggcaccgggg tgttgtccgg gttcttctcc tcgggcagcg ggtcgtcgtt gatgatcgcg
     2281 tcgaacagtg cccgcatgtc cccttcgcgc ggtgtctcgt tgccgtactc gtcggcgtag
     2341 ccaaccgtcg gcacggtgac aaacgtgatc cggcccgcgt tcacgccctg aatggactgg
     2401 ccgaggtcga cgaggtcctt ggtcttgatg ttgtcgacga agctgtcgtc gatgaacatg
     2461 ttgaccacgt tgttgagctt tgacagcgag aagaacactt ccttggagat cagcgaacgc
     2521 agcagtgacg acaggaacaa ctgctggcgc ttgatccgcc cgtagtcgcc gttggtctcg
     2581 gtggtcacct gccgggcgcg cacgtactgc agcgcagtgt ggccgtcgac catctggcgg
     2641 ccggcgttgg ccaacaccgt gcccagctcg tagtcctcca gcggcgtcgt gctgcacacc
     2701 tcaacgccgc cgatcgcgtc gaccatcttc gaaaatccgg cgaagtcgac tgccatgaac
     2761 cggtttacag aaaggccgga tagtttctgg accaccttca ccagacactt cgggccgccc
     2821 accgcatagg cagagttgat ctttgtttcg gtgtagacct ctttttgtcc gtacatttcg
     2881 gactgctcgt cggtgacggg cccatattgg ccggtgtcgg gatcccagtg ctcgcacttc
     2941 gttggcgtga tcgcgaggtc gcgcgggaat gacacggcga caacgcgttt gcggttggcc
     3001 gggatgttta ccagcatgat ggtgtccgac cgtgcgccgc ctgcgtcctc ggtggtgccc
     3061 gcgcccatgt cgctgttctc gccgaagcgg ctgtcgacgc cgacgatcaa gaagttctcg
     3121 tcaccgaatt gggcgttggg gtcgaggatg tcgcgagagt ccgggtccag cgcggagacc
     3181 ttgttgagca tgttgttctt tgacgactgc cactgccatg ccccgcctgt cagtgcgagc
     3241 gccgataccg caatgagcgc ggcggcggcc cgtccggcca ccaccgtctt ggtgaggtgc
     3301 ctccggcgcg gcttgggcgc aggagtgctg cgctcggtac cgacgcgtga cggctgcacc
     3361 cggtcacgct ggcgcagcgt cgccagatcc ggcacctccg acgcgtaccc cgccacaacg
     3421 gggataacct cggtgttcgg atcttctcgc tcgggacgga ccgccgcgat cacctcggtg
     3481 gccggggctg gcggttcggg ctcggggcgg ggccgcctca attcttcggg aacactcgat
     3541 gcgccgtgca ccttggcgat caggtcagcg accgtgactc catcggtgtg gttgccggtg
     3601 tgctcctcct cgttgatgtc tccggagtcg gaatggccgt tgcgctccca cggcgcggca
     3661 cctggcgacg gccgtgcgct tcgggtaagc caagggttgt cgccagcgcc gggcaggcca
     3721 ggagtggcgt tgtcgccgtc actcatgttc ctggtggcct ccggcttctt gaccatcgtg
     3781 cgcgtcggtc ggcatgggcg tatgcagtgt ctttgtctgc ctctaggtcg tgtagcagca
     3841 ttctgcgggc tccgccgttg gagccagata cgacaagagc cttatcgtac tgagacatcc
     3901 tgggagacgg ccagtccact gacggtaccg acaacgtctc ggtttagcct ccggagtgca
     3961 taacgtccgc gcccgcaggc acggtgcagt cgtctggatc ggctagccag ccctctggca
     4021 gtgccaccga cgcgggcgac ccctgccgtc cgcgcgggcc ggtcgccgcc tgcgggaacg
     4081 gcaccgccgg atcgagctgc tccagcagcg actcgagctc agccaacgtc ttgaccagtg
     4141 ccaatgcgcg tcgcagatcc gcgccggcgg gaaagccgtg caagtaccac gcgatgtgct
     4201 tgcgcatatc gcgcatgccc ttgtcctcgc cgaaatgcgc ggccaacagc tcgccgtggc
     4261 gccgcatgat ggccgcgacc tcgccgagag tgggcggcat cgccggcgtt gtgccggcaa
     4321 aagccgcgga gagctcggcg aacagccagg gccggcccag acagccgcgg ccgatgacga
     4381 ccccgtcaca ccctgtggtg gccatcatcg ccagggcgtc gtcggcgtcg aagatgtcgc
     4441 cgttgcccag caccgggacc gtcgtgacgt gctgcttgag cagggcgatc tggtcccagt
     4501 cggcggttcc cgagtagcgc tgcgccgcgg tgcgggcgtg tagcgcgaca gccgcagcgc
     4561 cctcctcggc ggcgattttg ccggcatcca ggtgggtgtg atgggcgtcg tcgatgccga
     4621 tgcggaattt cactgttacc gggatggtcg tgccctcagt ggcccgcact gccgccgcga
     4681 cgatcttgcc gaacagccgg cgcttgaacg gaagcgccgc gccaccgccg cgccgggtga
     4741 ccttgggcac cgggcagccg aagttcatgt cgatgtgatc ggccaggccc tcgtcgccga
     4801 tcattttcgc cgcggcatac gtgttctgcg ggtcaacgct gtagagctgc agcgagcgcg
     4861 gtgtttcgtc cggcgcgaac gtcgtcatgt gcatggtgac cggatggcgc tcgacgagcg
     4921 cacgcgccgt caccatctca cacacgtaca gcccgctgac cgtgcccgcc cgggccaact
     4981 cgagttcacg gcagagcgtg cggaatgcga cattcgtcac accggccatt ggggccagca
     5041 cgacggggct ccgcagctcg atcggcccga tgcgcagccc tgatcgggcc tcggttacga
     5101 cgctgatgac gtgaccaaga ggttcttcgc ggccttcttc tcggcgacct tgcgctcgcg
     5161 ttcgaggcgg cgctccttgg cgacctcgaa cttctcgcag gtgtcctcga gttccttcac
     5221 cagctcgccg aggtcgtcgc gcatcgctgc ggcctcgccg gtgaagtcct cgcgctcgaa
     5281 gatccgccac ttcttcagca ccggcatcac cacgtcgtcg aggtggattc gcgggtcgta
     5341 gacgccgccg accgcgatga tcacggcctt gcgccggaac tccggcacgg tgtaccccgg
     5401 catcttgaaa ttgcgcagca cgcggtgaag cgaagccatc gcctggttgg gggcgatatc
     5461 gaagccggcg tcggacacgt cgcggtagaa gatcatgtgc aggttttcgt ccgccgaaac
     5521 ccgttgcagc agttggtcgg cgatcgtctc ctggcatgcc ttgccagtgt tgcggtgcga
     5581 gacgcgggtg gcgagctcct ggaacgtcac atagatgacg gagtcgaaca ggctctccgc
     5641 gaacagctcg ccctgctgat tctggccagg ggagaagccg cgggtgacct gttcgacgcg
     5701 cagcaactcc agctccacgg gatcgatcgc gcgggtgacg acgaggtagt cacgcagcgc
     5761 gatgccgtgc cggttctcct cggcggtcca gcggttcacc cactgccccc acgggccgtc
     5821 catcccgaag ttcatcgcga tctcgcggtg gtaggacggc aggttgtcct cggtgagcag
     5881 attggtcagc atcgccacct gggcgacctc ggacagcttc gactcctcgg gatcccaatc
     5941 cttgccgccg agggcgtaat agttcttgcc gtcggaccac gggatgtaat cgtgcgggtt
     6001 ccagtcttta cgcatggtga ggtgccggtt gacgttcttc tc
//
LOCUS       DASRJA010000153         7585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3548937,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000153 DASRJA010000000
VERSION     DASRJA010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3548937"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFC01_07235"
     CDS             <1..549
                     /locus_tag="VFC01_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZN79465.1"
                     /translation="TVIVVALGIVVGVWMGRSPTADKIVRPFLDAAQTMPSFVYLVPF
                     LALFSASRFTGIVAAIVYAAPVSIKIMADGIRGVSAETVEAARSVGSNTWQVISKVQL
                     PMAVRSLTLATNQGLIYVLSMVVVAGLVGGGALGYLVVAGFAQTSLFGKGLAAGLAIV
                     LLGTILDRTTAAASKRIGRAGE"
     gene            564..1526
                     /locus_tag="VFC01_07240"
     CDS             564..1526
                     /locus_tag="VFC01_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN79466.1"
                     /translation="MAAALVLLISGCNATKVSDLQSRGELKPGTRPCGTFNLAINSWV
                     GYEANAAVITYLAENVLGCFVKQRHIAEQVSWQGMSTGQVDAILENWGHDDLRQQYID
                     NMGVAQSAGSTGIEGEIGWYVPPWMAEKYPDITDWRNLNKYAHLFQTSESGDKGQLLD
                     GDPAYVTNDDALVSNLNLNYKVVQGGSETALISSIRQAQQQRTPLLAYFYSPQWLLSE
                     VPMVKIDLPPYTDGCDADPAKIACDYPAYDLDKIVSTKFAESGSPAYTLVKNFQWTAE
                     DQNSVARSIAMDGMSDDEAAKKFIDAHPQLVAKWLAGTGAENAA"
     gene            1682..2035
                     /locus_tag="VFC01_07245"
     CDS             1682..2035
                     /locus_tag="VFC01_07245"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HZN79467.1"
                     /translation="MCPLAALPRGEARRVEADPPIAVFHTDDGEVFAIDDTCTHQDAS
                     LADGWLEGCEIECPLHASRFNLRTGEVDEPPAKLPVRTHEVVIVDGVINVVLDDAPPN
                     LPPGVATRAAGEAAQ"
     gene            2032..3201
                     /locus_tag="VFC01_07250"
     CDS             2032..3201
                     /locus_tag="VFC01_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN79468.1"
                     /translation="MKSVAVIGASLAGLSAARALRAQGFDGELTIVGGEVRRPYDRPP
                     LSKEFLAGAIGEDALALEADDDDLNARWLLGVQATGLDATAGAVQLDSGTTVAADGIV
                     VATGARARSWPASEGLAGVHVLRTVDDAIALRDELRSGARLVVIGAGFIGGEVASTAA
                     KLGLDVTVVEAALAPLAGPLGVQLGATVARLHTEHGTRLLCGAPVAGLIGSDRVTGVD
                     LADGRRLAADVVVVGIGAIPNIEWLRDSPLELANGVVCDEGGATSIPNVVAVGDCAAW
                     HEPSVGWPHRVEHWTGALERPAVAVATLLAGGRHNGRPVKPPYFWSDQYNRRIQFAGI
                     ADPGDEITFEVGGPRDTSFLAVYRRGGEPVAVLGVDQPKLFTRWRRQLTVVPVPA"
     gene            3198..4349
                     /locus_tag="VFC01_07255"
     CDS             3198..4349
                     /locus_tag="VFC01_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009474503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZN79469.1"
                     /translation="MTSIPVEEIAMTTVGLPQSLISTLPGSYYTDATIFSMEQAQIFE
                     SMWFCAVRSADLDKPGAFKTVQVGRENVLITRSRSGEVRAFFNVCRHRGAQLCTEETG
                     EVKRAFQCTYHAWTYDFDGKLVAAPNLTKMPDIDRVEYGLRRITVQEWLGYVWVCLAD
                     TPPSFDETVRKDVSDRLGSVDSIDHYDVANLRVGRRIRYDVKANWKLIIENFMECYHC
                     ATIHPELTEVLPEFADGLAVQYHVGHGAEFGEDVQGFTVDGSEGLDQIPGVTAEQDRR
                     YYAITIKPQVFVNLVPDHVIVHRMFPMAADHTIIECDWLYLPHVVESGKDVSRSVELF
                     DRVNQQDFAACERCQPAMSSRTYRDGGVLVPSEHHIGEFHHWVQAQLTK"
     gene            complement(4355..5134)
                     /locus_tag="VFC01_07260"
     CDS             complement(4355..5134)
                     /locus_tag="VFC01_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN79470.1"
                     /translation="MSNPRGESAGGVQSVDRAVSVLEILAQRGEAGVSEVAADIGVHK
                     STAFRLLAALEERELVEQTQDRGKYRLGFGILRLASAVPGQLDVTHQSRAICEQLAAE
                     LGETVNIAVRRSHFVVNIDQVRGPSAVATHNWVGELTPLHATSSGKVLLAYMTPEQRR
                     EVLTSSGLARLTAHTITSQRELEAQIEAAARDGYAISVEELEDGLNAVAAPIRDHTSG
                     VIAALSVSGPVYRFTEERMREVIPAIVAAAAAISQRMGHLD"
     gene            5213..6904
                     /gene="betA"
                     /locus_tag="VFC01_07265"
     CDS             5213..6904
                     /gene="betA"
                     /locus_tag="VFC01_07265"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HZN79471.1"
                     /translation="MTSTRYDFVIVGGGSAGCAIANRLSADPANKVLVLEAGRNDSLW
                     DVFVHMPAALPFPIGSRFYDWKYESEPEPHMHGRRVYHARGKVLGGSSSINGMIFQRG
                     NPMDYERWGADPGMGDWDFAHCLPYFNRMENCLAAEPDDPYRGHDGPLALERGPATNP
                     LFEAFFTAAEQAGYPRTDDVNGYRQEGFAKFDRNIRNGRRLSAARAYLHPVMNRPNLD
                     VVTRAFVERVVFQGKRAVGVEYRRFGSAKRVSAGQVVLCGGAINSPQLLQLSGVGNAG
                     ELQALGIDVVHDLPGVGENLQDHLEVYIQYACKQPVTMQKYLKWRYRPWIGANWLFLR
                     RGPGATNHFEGGGFVRSNDDVAYPNLMFHFLPIAVRYDGSQPEGDDGYQVHVGPMYSD
                     VRGTLKIVSRDPKVHPALRFNYLSTDQDRREWIEAIRVARNILNQPAMAPFNGGETSP
                     GPSVDTDEEILEWVARDAETALHPSCTAKMGTDAMSVTDPQTMGVHGVEGLKVVDASV
                     MPYVTNGNIYAPVMMVAEKAADLILGNQPLAPATVPFYRHQPKQQPSDSEPVGRN"
     gene            6917..7363
                     /locus_tag="VFC01_07270"
     CDS             6917..7363
                     /locus_tag="VFC01_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HZN79472.1"
                     /translation="MSVDRGAWALTDRRYTDSHSWLALPAEQRLGARPLRVGITQAAV
                     GEIHVISVELPRVGTAVRAGELCALIWSRPLSAMPVYAPITGVVSAVNGAVRADPGVV
                     ARDPLHAGWLFTVLPSGESSTDELLTASDYADSLGIGAPASAERRS"
     gene            7360..>7585
                     /locus_tag="VFC01_07275"
     CDS             7360..>7585
                     /locus_tag="VFC01_07275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005252394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN79473.1"
                     /translation="MSTPRVVIIGAGIVGANLADELTARGWDQVTVVDQGPLPLTGGS
                     TSHAPGLVFQTNASKTTAEFASYTVEKFVGM"
BASE COUNT         1327 a         2438 c         2508 g         1312 t
ORIGIN      
        1 acggtcatcg tcgtcgcgct cgggatcgtg gtcggcgtgt ggatgggccg cagcccaacc
       61 gcggacaaga tcgtgcgacc gttcctcgac gccgcacaga ccatgccgtc cttcgtgtat
      121 ctggtgccgt tcctggcctt gttctcggcg tcgcggttca ccggcatcgt ggcggcaatc
      181 gtctatgccg cgccggtgtc gatcaagatc atggccgacg ggatccgcgg agtctcggcc
      241 gagaccgtcg aggcggcgcg ctcggtcggc tcgaacacct ggcaagtcat cagcaaggtg
      301 cagctgccga tggccgtgcg ctcgctaacg ttggccacca accagggcct tatatatgtg
      361 ctctccatgg tcgtggtcgc gggtctggtc ggcggcggtg cgctgggcta tctggtggtg
      421 gccggcttcg cgcagaccag cctgttcggc aagggcttgg cggcaggcct ggcgatcgtg
      481 ctgctgggca cgatcctcga ccgcacgacc gcggccgcat cgaaacggat aggacgggca
      541 ggcgaatgag acgaattgcc atcgtggctg cagcgcttgt gctgctgata tcgggctgca
      601 acgccaccaa ggtgtcggac ctgcaaagcc gcggcgaact gaagccgggc accagaccgt
      661 gcggcacctt caacctggcc attaattcat gggtcgggta cgaggcgaat gcagcagtga
      721 tcacgtatct cgcggagaac gtgttgggct gcttcgtcaa gcagagacac atcgccgagc
      781 aggtctcctg gcagggcatg tcgaccgggc aggtggacgc gatcctagag aactggggtc
      841 acgacgacct gcggcagcag tacatcgaca acatgggcgt cgctcagagc gccggctcta
      901 ccggcatcga gggcgagatc ggctggtacg tgccgccgtg gatggccgag aaatacccgg
      961 acatcacaga ttggcgcaat ctgaacaagt acgcccacct gtttcagacc tcggaatccg
     1021 gtgacaaggg gcagctgctc gacggcgatc cggcctatgt gaccaacgac gacgctctgg
     1081 tctccaatct gaacctgaac tacaaggtgg tgcagggcgg cagcgaaacg gcgctgatct
     1141 ccagcattcg tcaggcacag caacaacgga ccccgctgct cgcgtacttc tactcgccgc
     1201 agtggttgtt gagcgaggtg ccgatggtga agatcgacct gccgccttac acggacggct
     1261 gcgacgccga cccggccaag attgcctgcg actacccggc gtacgacctg gacaagatcg
     1321 tctcgaccaa gttcgccgaa tccggcagtc cggcctacac gctggtgaag aacttccagt
     1381 ggacggccga agaccagaac tccgtcgccc gctcgatcgc aatggacggg atgagtgacg
     1441 acgaggcggc caagaagttc atcgatgcac acccgcagct tgtggccaag tggctggccg
     1501 gcaccggagc cgagaacgca gcgtagtcac cgctgttcag ggtgggtgcg cgacgtcaaa
     1561 actcgcgggc cgcagctctt gacaaggccc ggccatttcc atcaggctgt tgcatattac
     1621 gaatcgagtt tcataatccg ctacaaagca cctacttggg gaggttgagg tcgtgctcga
     1681 aatctgtcca cttgcggccc tgccccgcgg cgaggcgcgg cgggtcgaag cggacccgcc
     1741 gattgccgtc ttccacaccg acgatggcga ggtgttcgcg atcgacgaca cctgcacgca
     1801 tcaggacgcg tcactcgccg acggctggct ggagggctgc gagatcgaat gcccgctgca
     1861 cgcttcgcgt ttcaacctgc gcacgggtga ggtcgacgag cctccggcga agcttccagt
     1921 gcgcacccac gaagtggtca tcgtcgacgg ggtgatcaac gtcgtgctcg acgatgcccc
     1981 gccgaacctt ccccccggcg tcgccacccg cgcggccggc gaggcggcgc agtgaaaagc
     2041 gttgctgtga ttggggcttc gctggcgggg ctctcggctg cccgcgcgtt gcgcgcacag
     2101 ggtttcgacg gcgaactgac gatcgtcggc ggtgaggtgc gacggcccta cgaccgtccc
     2161 ccgctgtcaa aggagtttct tgccggtgcc atcggcgagg acgccttggc tctggaagcc
     2221 gacgacgatg atctgaacgc ccggtggctg ttgggcgtgc aagcgaccgg cctcgacgcg
     2281 accgcgggcg ccgtgcagct cgacagcggc accacggtcg ccgccgacgg catcgtcgtg
     2341 gcgaccggag cgcgggcacg gtcgtggccg gcctcggagg ggctcgccgg cgttcacgtg
     2401 ctgcgcacgg tggacgacgc catcgcgttg cgcgacgaac tgcgctcggg ggctcggctg
     2461 gtggtgatcg gcgccggatt catcggtggc gaagtcgctt ccacggcagc gaaattgggc
     2521 ctcgatgtca cggtggtcga ggcggcgctg gcgccgctcg ccggcccact tggcgtgcag
     2581 ctaggtgcca cagtggctcg gctgcacacc gagcacggca cccggttgct gtgcggggct
     2641 ccggtcgcgg gtctcatcgg cagcgatcgc gtgaccggtg tggatttggc cgacgggcgc
     2701 cggctcgccg ctgacgtcgt cgtcgttgga atcggcgcca ttccgaacat cgagtggctg
     2761 cgtgacagtc cgctcgagtt ggctaacggc gtcgtatgcg acgagggcgg cgcgacgtca
     2821 atcccgaatg tggtcgcggt gggcgactgc gcggcttggc atgagccgtc cgtcggctgg
     2881 ccacaccgtg tggagcattg gacaggcgcg ttggagcgcc ccgccgtcgc ggtcgcgacg
     2941 ttgctcgccg gtggacggca caacgggagg ccggtcaaac ccccgtactt ctggtctgac
     3001 cagtacaacc gccggatcca gttcgccggg atcgccgatc ccggcgatga gatcaccttc
     3061 gaggtgggcg gcccgcgcga caccagcttc ctcgccgtat atcgccgcgg tggtgaaccc
     3121 gtcgccgtgc tcggcgtcga ccaaccgaag ctgttcaccc ggtggcgtcg ccaactgacc
     3181 gtcgtccccg ttccagcatg acttcgattc cagtagagga gatcgcaatg accaccgtcg
     3241 gcctgccgca gagcttgatc tcgacgctgc ccggcagtta ctacaccgac gccaccatct
     3301 tctcgatgga gcaggcccag atattcgagt cgatgtggtt ctgcgccgtg cggtccgccg
     3361 acctcgacaa gcccggcgcg ttcaagacgg ttcaggtcgg acgggagaat gtgctgatca
     3421 cccggtcccg cagcggcgag gtgcgcgcgt tcttcaatgt ctgccggcac cgtggtgcgc
     3481 agttgtgcac cgaggagacc ggtgaggtca agcgcgcgtt ccaatgtact taccatgctt
     3541 ggacttacga cttcgacggc aagctggtcg ctgcgccgaa cctgaccaag atgcccgaca
     3601 tcgaccgggt cgagtacggc ctgcgccgca tcacggtgca ggagtggctg ggctacgtgt
     3661 gggtgtgcct cgcggatacg ccaccgtcgt tcgacgagac cgttcgcaag gatgtcagcg
     3721 atcgcctcgg cagcgttgac tcgatcgacc attacgacgt cgcgaacctg cgcgtcggtc
     3781 gccggatccg ctatgacgtc aaggcgaact ggaagctcat catcgagaac ttcatggagt
     3841 gctaccactg cgccaccatc catccggagc tcaccgaggt gttgcccgag ttcgccgacg
     3901 gactggcggt gcagtaccac gtcgggcacg gtgccgaatt cggtgaggac gtccagggat
     3961 tcaccgtcga cggctcggaa ggactggacc agatccccgg cgtcaccgcg gagcaagacc
     4021 ggcgctacta cgcgatcacg atcaagccgc aggtgttcgt gaacctggtg cccgaccacg
     4081 tgatcgtgca ccgaatgttc cccatggcag cggaccacac aatcatcgag tgcgactggt
     4141 tgtacctgcc ccatgtcgtg gaatccggca aggacgtcag ccgctcggtc gagttgttcg
     4201 accgagtcaa tcagcaggac ttcgccgcat gcgagcgctg ccaaccggcg atgagctcac
     4261 gcacatatcg tgacggcggg gtgctggtgc ccagcgagca tcacatcggc gagttccacc
     4321 actgggtgca ggcacagctg acgaaatgac cgcgtcagtc gaggtggccc atccgttggc
     4381 tgatcgccgc cgcggcggcg acgatagctg ggatcacctc gcgcatccgc tcttcggtga
     4441 atcgatagac cggacccgaa acactaagcg ccgcaatgac gccgctggtg tgatcacgta
     4501 tcggggcggc gacggcgttg agcccgtcct ccagttcctc gaccgagatc gcgtatccgt
     4561 cccgggcggc cgcctcgatc tgcgcctcga gctcccgttg cgacgtgatc gtgtgcgcgg
     4621 tgagtcgggc aagtcccgag gaggtcaaca cttctcggcg ctgctcgggt gtcatgtacg
     4681 ccagcagcac cttgccgctg gaggtggcgt gcaacggcgt cagctcaccg acccagttgt
     4741 gcgtggcgac cgcactcggc ccgcgcacct ggtcgatgtt cacgacgaag tgcgaccggc
     4801 gcaccgcgat gttgaccgtt tcgccgagct cagcagcgag ctgttcgcag atcgcgcgcg
     4861 actgatgggt gacatccagt tgacccggca ccgcgctggc cagtcgcagg atgccgaagc
     4921 ccaggcggta cttgccacga tcttgggtct gttcgacgag ttcgcgctcc tcgagcgccg
     4981 cgagcaaccg gaatgctgtc gacttgtgca cgccgatgtc cgcggcgacc tcgctcacgc
     5041 cggcttcacc acgctgggcc aggatttcca gcacgctgac ggcgcgatcg acggactgca
     5101 caccgccagc gctttcaccc cgcgggttgc tcataacgca actatacgag acgccgttgc
     5161 acatagagaa acgtaatgcg tatcacgctt caatagcgat aggagaacag ccatgacatc
     5221 gacgcggtac gacttcgtga tcgtcggcgg cggatcggcg gggtgcgcga tcgccaaccg
     5281 cctgtcggcc gacccggcca acaaggtgct ggtgctggag gcgggccgca acgattcgtt
     5341 gtgggacgtg ttcgtgcaca tgcccgcggc actgccgttt cccatcggaa gtcggttcta
     5401 tgactggaaa tacgagtccg aacccgagcc acacatgcac ggccggcgcg tctaccacgc
     5461 ccgcggcaag gtgctcggcg gctcgagcag catcaacggg atgatcttcc agcgcggcaa
     5521 cccgatggac tacgagcgct ggggcgctga cccgggtatg ggcgactggg acttcgcgca
     5581 ttgcctgccg tacttcaacc gcatggagaa ctgcctggct gcggaacccg acgacccata
     5641 ccgcggccac gacggtccgc ttgcgctcga gcgcggaccg gccaccaatc cgttgttcga
     5701 ggcgttcttc accgccgccg agcaagcggg ttacccgcgc acggatgacg taaacggtta
     5761 tcgccaagag ggtttcgcga agttcgaccg caatatccgc aacggtcggc gcctctcggc
     5821 cgcccgcgcc tacctacatc cggtgatgaa ccgaccgaac ctggatgtgg tcaccagggc
     5881 gttcgtcgag cgggtcgttt tccagggcaa gcgcgcggtt ggcgtcgagt atcggcgttt
     5941 cggctccgcg aagcgcgtca gcgccggcca ggtcgtgctg tgcggcggtg ccatcaacag
     6001 cccgcagctg ctgcagctct cgggcgtcgg aaatgccggc gaactgcagg cgctcggcat
     6061 cgacgtcgtc catgacctgc cgggcgtcgg cgagaacctt caggaccacc tcgaggtgta
     6121 catccagtac gcgtgcaagc agccggtgac catgcagaag tacctgaagt ggcggtaccg
     6181 gccatggatc ggcgccaact ggctgttcct gcgccgcggg ccgggggcga caaaccattt
     6241 cgagggtggg ggctttgtcc gcagcaacga cgacgtcgcc tatccgaacc tgatgttcca
     6301 tttcctgccg atcgccgtgc gctacgacgg ctcccagcca gagggcgacg acgggtatca
     6361 ggtgcacgtc ggcccgatgt actccgatgt ccgcggcacc ctcaagatcg tcagccgtga
     6421 cccgaaagtg catccggctc tgcggttcaa ctacctgtca actgatcagg accgccggga
     6481 gtggatcgag gccatccggg tggcacgcaa catcctcaac cagccggcca tggcgccgtt
     6541 caacggcggc gaaacctcgc ccgggccaag cgtggacacc gacgaggaga tcctcgaatg
     6601 ggtggcgcgc gacgccgaaa ccgcgctgca cccgtcatgt accgccaaga tgggcaccga
     6661 cgcgatgtcg gtgaccgatc cgcagacgat gggcgtgcac ggcgtggagg ggctgaaagt
     6721 tgttgatgcg tcggttatgc cgtatgtgac caacggcaac atctacgcgc cggtgatgat
     6781 ggttgccgag aaggcggcag acctgattct cggcaaccag ccgctggcgc ccgcgacggt
     6841 gccgttctac cgacaccagc ccaagcaaca gcccagcgac tcagaaccgg tcggccgcaa
     6901 ctgattcggc atcgacatgt cagttgaccg cggcgcgtgg gccctgaccg accgccgata
     6961 cacggatagt cacagctggc tggcgctgcc agctgaacag cggttgggcg cccgtccact
     7021 acgagtgggt atcacccagg ccgccgtcgg ggaaatccat gtcatctcgg tggaacttcc
     7081 gcgagtcggc acggcagtaa gggcgggaga gctgtgtgcg ttgatttgga gccgccccct
     7141 cagcgccatg ccggtgtacg cgccgatcac cggggtggtc tccgccgtca acggcgcggt
     7201 gcgcgccgat cccggcgtcg tcgcccgcga tcccttgcat gccggctggc tgttcaccgt
     7261 tctgccgtcg ggcgaatcgt ccacggatga gttgttgacc gcctcggact atgcggattc
     7321 tctcggcatc ggggcacccg cttcagctga gaggagatca tgagcactcc gcgtgtcgta
     7381 atcatcggcg ccggcatcgt cggcgcgaat ctggccgatg aactcaccgc acgcggctgg
     7441 gatcaggtca ccgtggtgga ccaaggtccc ttgccgctga cgggcggctc aacgtcgcac
     7501 gccccgggtt tggtcttcca aaccaatgca tcgaaaacga cggcggagtt cgccagctac
     7561 acggtcgaga agttcgtcgg catgg
//
LOCUS       DASRJA010000154        13606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1577615,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000154 DASRJA010000000
VERSION     DASRJA010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13606
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577615"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1720
                     /locus_tag="VFC01_07280"
     CDS             <1..1720
                     /locus_tag="VFC01_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN79474.1"
                     /translation="AVKLIVTLAPNEPVPDEISALWRDDLLARIDVAPAGHDDSRQAT
                     LVEEFVATLPGPARRVLEYLAVADPLSLADVSALAGRDAVTEAEAAGAVDIDEDRVRP
                     VHPLFVDAVRDALGGPVLRRLRTEVVDRLAASPALGVADRLRLAVLALDSDSPQPVAD
                     VAAAAEEALRLGDLELSERLGRAAVERSSELAIRLTLGYALAWQGRGREADSVLADVD
                     PSTLSETELMAWALPRAANQFWMLSEPERATAFLRTTRGRVSTPAAQTTLDALSATFA
                     MNAGSPSRAKQIADEVLASPAADDTAVGWAAAAAALSSARMGRFGEVDALAERAIAAG
                     HPGLLRFTSGFGQTTALVMAGELDRAQALAQQLTDFAQLQQPGRAIGEVLVADVLIAK
                     GELESAVSLLRGAAAALAPTGYSWGPLAWMLLAQALGQLGETVEAGKVLTRAESRHGL
                     KSMLFAPELSLARAWTLWARRDEHGAVGAARDAAKAAERGGQTAVALRALLDAVRLGD
                     IRAADGITRLCGEVDCVIGQLALQHARALVAKDAAALNDVSARFDAIGMKRAAAAAAA
                     QADSAQ"
     gene            1794..2870
                     /locus_tag="VFC01_07285"
     CDS             1794..2870
                     /locus_tag="VFC01_07285"
                     /inference="COORDINATES: protein motif:HMM:NF039175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EspA/EspE family type VII secretion system
                     effector"
                     /protein_id="HZN79475.1"
                     /translation="MGVLDAFLSTWSNARSTFGEGTPQTGAQFDNSVSLRHMQATVQS
                     AAPGSNWTGGAANAYGAANAAHAEVFGKIADLDQRLSAKVDQSAQVVGTGRRNLDTVR
                     QWVLDAAAAVPPGKNREQMLMPIVQRGLSQLSQIVTKSNGDLNKIGGEIVGLRAEYDA
                     LSNQRFAVQNRESGTGDQRKPVIQAIDFRESPAPDEPPPPDRGGGYGSYHYGYQFSTS
                     EAWSQQQIMQEVLNNYNKYFTFSGDQPKIVEGAVVNLNGPAGPEPVKVTKVTDTSFSF
                     VSLPGHHEGAGRVIEFSIVPAAASPVPGRLNWELRVEASGPVGGLSAIPGANWFNKGV
                     WQVFADNLNSRLPVSPPSSGGVTV"
     gene            2874..3155
                     /locus_tag="VFC01_07290"
     CDS             2874..3155
                     /locus_tag="VFC01_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79476.1"
                     /translation="MKVEVIERSAAVLIGMAAGYVLWLAGITALTAVVPMRYIIIAAA
                     ILLSVIVIASIALALHFKKTQRRSVALGFWWAPALPTVASFYSLIVFLS"
     gene            3214..3957
                     /locus_tag="VFC01_07295"
     CDS             3214..3957
                     /locus_tag="VFC01_07295"
                     /inference="COORDINATES: protein motif:HMM:NF022280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion target"
                     /protein_id="HZN79477.1"
                     /translation="MSDPLYVQPDGVRSYAQIHDQVLSALSQVMGAAAPAAVGVESTH
                     GPVASAVSTALDQVLGGRRGTLRATSTSAQTISELLQKAAQMYEQGDQQGAEKLKAAA
                     EALEGQQGGQPGNAGAPGGPTSTGGGDAMGQMLGQLGQVGQQVGQLAQAAGAPLQALA
                     QPLQQVPQQVMQAVQQASQSASKAADATGAAKGDPAVPQQDSRSEETERRNEPLHRAE
                     AAPGDSANTGRAPETPEHAKPAQTRPQTD"
     gene            complement(3963..4667)
                     /locus_tag="VFC01_07300"
     CDS             complement(3963..4667)
                     /locus_tag="VFC01_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79478.1"
                     /translation="MIDPLRKPEGITWDPASVELPEIPMLPAGQDALSATISAVLPTL
                     AVPLAANVATLQAKETMFSGKIGAAQAAYQNSDDAGAQSVGQIVGMLGQVGQQAGQMG
                     QMAGAPAQALGGQTGMFGSLMQQAMQGAQGGGAGGQSAGGQGSSAAGAPPTGAVGAAG
                     AVGQPQPRDDGVDTKDEQPTRAHEPRHEEREPLQRADDRAMAGPSGAEDGRHGQAPVG
                     PSHQPRHGDEDLGRRL"
     gene            complement(4671..5801)
                     /locus_tag="VFC01_07305"
     CDS             complement(4671..5801)
                     /locus_tag="VFC01_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79479.1"
                     /translation="MPIEADSSPYGSQTVTGPAWPNVDEEMLAAAAATYEALAANITG
                     SIVPLQQGQLMKLADVWEGAGSLAATGEATTIIGGHEANAAHAAAIAGKLRAMEATVV
                     KAKMLANTIAQEVQHECEALSAMPFSNTQELVQSRIKMGLSQNIANVTTHTTELANTL
                     GVPPSIPFPGAAPSVPGGKETKDTAEEAAKQAGGGSQQAMQMMSQMGSMAAQIPMQLG
                     QAVMAVPQQMGQQLQQLSQPLQQLTSMFGSGGKADSLGAAGLPFSSFSNHPLAGGSGA
                     GGGSGMVKAASLPGSGGTPLQTAQMANLVGTKSVSTAPAEGATAGSTATSGVAPVAAG
                     SGMGGMAPMMGHRGESGGTSASLAVPTPLEHDLDEGDDDDDW"
     gene            complement(5868..7481)
                     /locus_tag="VFC01_07310"
     CDS             complement(5868..7481)
                     /locus_tag="VFC01_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZN79480.1"
                     /translation="MSSIVCSNLSFSWPDDTPLFTGLSFTVGDGRTGLVAPNGAGKST
                     LLKLIAGEYRPSAGTITVDGTVGYLPQTLPFVADRSVADALGVAPVLDALTALAAGDA
                     GEDVFVAIGDDWDVEERTQAQLDRLGLGHITFDRRLGSLSGGEVVSLGLAAELLKRPD
                     VLLLDEPTNNLDADARHRLYDALDDFPGCLLLVSHDRVLLDRMDRIAELRRGEMLSYG
                     GNFTMYQQSVQEAQRVAEANLRNAEQQLKREKRQRQQARERAARRSSTAARNVKDAGL
                     PKIIAGAMKRGAQESAGRSDDVHAKRVNQVKAQLDDAERALRDDDMIVLDLPDTIVPA
                     GRTVFRGENLQICHGGRKLFVNNGIDLNIRGPERIALTGHNGAGKSTLLRVIDGSLQP
                     DGGTLARADGRTAYLSQRLDLLDPDRTVAQSLAAFAPSLTHTRRMHLLAQFLFSGNRI
                     HLPVAALSGGERLRATLACVLYAEPAPQLLLLDEPTNNLDLVSVGQLEAALNAYKGSF
                     VVVSHDERFLAGIGIQRRLRLSEGRLESQ"
     gene            complement(7560..8627)
                     /gene="dapE"
                     /locus_tag="VFC01_07315"
     CDS             complement(7560..8627)
                     /gene="dapE"
                     /locus_tag="VFC01_07315"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HZN79481.1"
                     /translation="MGLDLRGDPIALTAALVDIPSESRHEQRIADEIEAALREQTTGF
                     EVVRHGDTVLARTNLGRPSRVLLAGHTDTVPAADNLPSRLDGDTLHGCGTSDMKSGDA
                     VFLHLAATVADPKHDLTLVMYDCEEIEAAANGLGSIERELPDWLHADVAILGEPSGGF
                     IEAGCQGTLRVIVSAAGTRAHSARSWLGDNAIHKLGEVLARLKAYQPRDVDIDGCVYR
                     EGLSAVRIDGGIAGNVIPDAASVTVNFRFAPDRSVEQATDHVREVFDGLDVHIELTDS
                     AAGALPGLTKPAAAALVDAAGGQVRAKYGWTDVARFAALGIPAVNYGPGDPNFAHRAD
                     ERVEVAQISTVTDMLRRYLTA"
     gene            8662..9606
                     /gene="dapD"
                     /locus_tag="VFC01_07320"
     CDS             8662..9606
                     /gene="dapD"
                     /locus_tag="VFC01_07320"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HZN79482.1"
                     /translation="MTSAAGVGLATIAADGSILDTWFPEPELGAGGPTGTARLSIAEI
                     PPELAELTGHDHDRDVETVAVRTTIASLEDKATDAYDAYLRLHLLSHRLVAPHGLNAD
                     GFFGVLTNVVWTNYGPCAVEGFEVVRARLRRRGPVTVYGIDKFPRMVDYVVPSGVRVA
                     DADRVRLGAHLASGTTVMHEGFVNFNAGTLGSSMVEGRISAGVVVGDGSDVGGGASIM
                     GTLSGGGTQVISVGKRCLLGANAGLGISLGDDCVVEAGLYVTAGTKVQTADGKTVKAR
                     ELSGASNLLFRRNSLSGAVEVVKRDGTGITLNEALHHN"
     gene            9757..11037
                     /locus_tag="VFC01_07325"
     CDS             9757..11037
                     /locus_tag="VFC01_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HZN79483.1"
                     /translation="MTQPTVALFPEPGAWGPTNNLVAVGNHLRERGIRTVFVVEESFD
                     GELAKRGFEERLMRMAPPPEGEETVGGGWAEFVRETSPQFRRPTIEQLETVTAPIWAE
                     FVDGAEYSHERLTEIWSELRPDAIVHDCVASFPSVSLAGCPWIRVVSANPLEITDPDI
                     PPVFSGYPSRDRSGWDEFWVEYRRLTDPLVERASAFHQQCGAPPLPDGEFQYESPYLN
                     LYMYPAELDYQRSRPLGATWHRIDTSTRTSDGAFDVTEKVGSDGKLVYLSLGSLGCMD
                     VPLMQRVIDSLSQTSHRVIVSMGPLHDQLTLGDRMWGEQFVPQTAIVPHCDVMITHGG
                     NNTVGEAFTFGVPTIVLPLFWDQYDNAQRIDECGYGVRLSTYEFADDQLHGAIERLSG
                     DRQLAARLDAASKRLQASPGRVVAADLIEKIARG"
     gene            complement(11041..12228)
                     /locus_tag="VFC01_07330"
     CDS             complement(11041..12228)
                     /locus_tag="VFC01_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HZN79484.1"
                     /translation="MAQDPGQAAQMRWLGASQLIGVVRSEPGITRAVAAQRLGIGSGG
                     ATELVARMRQTRLLDETPAPAQGRGRPTTVLGPHADGPLVLAADLRATDWRLALADID
                     GLPRVVAEAEYAEADLYAVLTQLADAIGGIYRRRPKRLRAIAVSAAGPISGARLGQFT
                     PRGWNDVDLSAMTAKIPDRAGVQLLLGNDATLAGLAEARTGAARSAATALYLMVAEGI
                     GGALVVNGEPLTGAHGAAGEYGHVPFGDPALECPCGARGCWDLTVDGRALARHRGDAP
                     PDDAVAYVHELIHRPTRDKLTRRAFEAVAASLGRGTGAYVNVHDPDVVTLGGVAALLR
                     AEAPEAFDDAYRNALMAFRKGSPPPVRDAEHGEDGPLHGAVALALDHVTSPAGLADWV
                     GRH"
     gene            12282..13460
                     /locus_tag="VFC01_07335"
     CDS             12282..13460
                     /locus_tag="VFC01_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN79485.1"
                     /translation="MTLAADRVTGQGLRESLQGLLSLALASCLAVTTEVLPVGLLPDI
                     GATFGVSESVTGLLVSLYAVMVATLAVPLTLATSRFARKPLLQTTLLCYAVSNALVAA
                     APVFAVVGVGRTVGGITHALFFSLVIGYAPRLVSRAHVGRALAVAASGASTGMVLGVP
                     LSTSLGNAFGWRVSFAVLAALSVLAFVLMSRLLPSVSHEPLSGGSVGAGRGALVAISA
                     SNLLAFLGQFTVYTFVIVLLLSSGVSPAFVGPILLACGAGGLLAVWWAGRNLDRSPRR
                     AALVLLAIVIGAVVALGGTWPTLVAVVVATVVWNGAFGGLPSVYQTCAVRTHAASPEL
                     AGAWVNATANAGIAGGAAIGAGLLHAAGLSSLPWVGASLIGLGLAVVVLSRKAFPARA
                     "
     gene            complement(13465..>13606)
                     /locus_tag="VFC01_07340"
     CDS             complement(13465..>13606)
                     /locus_tag="VFC01_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M15"
                     /protein_id="HZN79486.1"
                     /translation="VEADKWLIRNGTQFGLCQIYANELWHFELAADPQGNCPPLRPNA
                     AG"
BASE COUNT         2116 a         4437 c         4691 g         2362 t
ORIGIN      
        1 cgcggtgaag ctgatcgtca ccctcgcgcc gaacgagcct gtccccgacg agatttcggc
       61 gctgtggcgc gacgacctgt tggcgcggat cgatgtcgca ccggccggcc atgacgacag
      121 caggcaggcc acgttggtcg aagagttcgt cgcgacgctg cccggcccgg cgcgccgggt
      181 gctggagtat ctggccgtcg cggatccttt gtcgctcgcc gacgtctctg cgcttgcggg
      241 ccgcgatgcc gtcaccgagg ccgaggcggc aggcgccgtc gacatcgatg aggaccgcgt
      301 ccggccggtg caccccctgt tcgtcgacgc cgtccgcgac gcgctcggcg gacctgtgct
      361 gcgcaggctg cgcacggagg tggtggatcg gctggcagcc tcgccagcac tcggcgtcgc
      421 ggaccgattg cggctcgccg tgcttgcgct ggacagcgac agcccgcagc ccgtcgccga
      481 cgtggccgcc gcggccgagg aggcgctgcg gctgggggac ctcgaactca gtgaacgttt
      541 gggccgcgcg gcggttgagc gctcgtcgga gctggcgatc cggctgacgc tgggctatgc
      601 gctggcgtgg cagggccgcg gccgcgaggc cgattcggtg ctggcggacg tagatccgtc
      661 gacgctgtcc gagaccgagc tgatggcgtg ggcattgccg cgcgcggcga accagttctg
      721 gatgctgtcc gaaccggagc gcgcgaccgc gttcttgcgg acgacgcgtg gtcgggtgtc
      781 gacaccggcc gcgcagacga ccctcgatgc gctgtcggcg acgttcgcga tgaacgcggg
      841 cagccccagc cgcgccaagc agatcgccga cgaggtgctg gcgtcaccgg cagccgacga
      901 caccgcggtg gggtgggcgg cggccgcagc ggcgctgagc tcggcgcgga tgggccgatt
      961 cggtgaggtg gacgcgttgg ccgagcgtgc gatcgccgcc gggcatcccg gtctgctgcg
     1021 gttcaccagt ggtttcggtc agacgacggc gctggtgatg gccggcgagc tggaccgggc
     1081 ccaggcgttg gctcagcagc tcaccgactt cgctcagctg cagcagccgg gccgcgcgat
     1141 cggtgaagtg ttggtggccg atgtgctgat cgccaagggc gagctggaat cggcggtgtc
     1201 gctgttgcgc ggcgccgcgg cggctttggc accaacgggg tattcatggg ggccgctggc
     1261 gtggatgctg ttggcgcagg cgttggggca gttgggcgag actgtcgaag cgggaaaagt
     1321 gttgacgcgc gcggaatctc ggcatggcct gaagtcgatg ctgttcgcgc cggagctttc
     1381 cttggcgcgc gcgtggacct tgtgggcgcg gcgcgacgag catggggcgg tcggagcggc
     1441 gcgcgacgcc gcgaaggcgg ccgaacgcgg cggccaaaca gcggtcgcgc tgcgcgcact
     1501 gctcgacgcg gtgcgactgg gcgacatccg cgcggctgac ggcatcacgc ggctctgtgg
     1561 cgaagtcgac tgcgtcatag gccagttggc gcttcagcac gcgcgagcct tggtcgccaa
     1621 ggatgccgcg gcgcttaacg acgtgtcggc gcggttcgac gcgatcggca tgaagcgcgc
     1681 ggccgccgca gcggcggcgc aggccgacag cgcccagtaa cggaatttgg tgcgggaggc
     1741 aaggtccggc ggcccctaga attttgttag ctagtcgctt tgttggaggt gctgtgggcg
     1801 tactcgacgc gttcttgtcg acgtggtcga acgcacgatc aaccttcggc gaaggcaccc
     1861 cacaaaccgg ggcgcagttc gacaacagcg tatcgctgcg acatatgcag gccacagtgc
     1921 agtcagccgc cccgggctca aactggaccg gcggcgcggc caatgcgtac ggcgccgcca
     1981 acgccgccca cgcggaggtc ttcgggaaga tcgccgacct ggatcagcgg ctcagcgcga
     2041 aagtggatca gtccgcacaa gtcgtcggca ccggccggcg caacctcgac acggttcgtc
     2101 agtgggtgct cgacgccgcc gcggcggtgc caccaggcaa gaaccgcgaa cagatgttga
     2161 tgccgattgt ccagcggggc ctcagtcagc tctcccagat cgtcaccaag tccaacggag
     2221 acctcaacaa gatcggcggc gaaatcgtcg gcctccgtgc tgaatacgac gcgctcagca
     2281 atcagaggtt cgctgttcaa aaccgcgagt caggaaccgg agatcagcgg aaacccgtga
     2341 ttcaagcgat cgacttccgt gagagtcccg ctccggacga gcccccgcca cccgatcgag
     2401 gagggggcta tggcagttat cactatggat atcagttctc gaccagtgag gcttggtcgc
     2461 agcagcagat catgcaagag gttctgaaca actacaacaa atacttcacg ttcagtggcg
     2521 atcagccgaa aatcgtcgag ggtgctgttg tcaacctcaa cgggcctgct gggccggagc
     2581 ctgtgaaagt aacaaaggtt accgacacga gcttttcatt cgtatcgttg cccggccacc
     2641 acgaaggggc cggtcgtgtc atcgagttca gcattgtgcc agcggccgcc agcccggttc
     2701 ccggacgatt gaactgggag ctgcgcgttg aagcttccgg cccagtggga ggcctcagcg
     2761 ccattcctgg cgctaactgg tttaacaaag gagtttggca ggtgttcgcg gataatctga
     2821 acagccgatt gccggtatcc ccacccagtt ccggcggagt gacggtctag aaaatgaagg
     2881 tggaagtgat cgaacgatcg gcagcggtgc tcataggcat ggcagcaggc tacgtattgt
     2941 ggctcgctgg tataactgcg ctgactgccg tcgttccaat gcgatacata atcatcgctg
     3001 ctgcaatcct tttgagcgtg atcgtaattg cctccattgc cctagctcta catttcaaga
     3061 aaacacaaag acggtctgtt gcgcttggct tctggtgggc acctgcgcta ccgacggtgg
     3121 ccagcttcta ctcccttatc gtttttctga gttgacgtga aggatgtcga atgatgaagt
     3181 ccgacaacag ctttgcgctg gtagaggcgc gagatgagtg acccgttgta cgtacagcct
     3241 gacggggtgc gctcttacgc acagatccac gaccaggtgc tgtctgcctt gtctcaggtg
     3301 atgggagcgg ctgcacccgc ggcagtcggc gtcgaatcca cgcacggccc ggtcgcgtca
     3361 gcggtcagca ccgcgcttga ccaggtcctg ggcggccgcc gaggcacgtt gcgggcgacc
     3421 tcgacgtcgg cgcagacaat ctccgaattg ctccagaagg ccgcccagat gtatgagcag
     3481 ggcgaccagc agggcgccga gaagctgaag gcggcggccg aggccctcga aggacagcag
     3541 ggcggccagc caggcaatgc gggcgcaccc ggaggcccca catccacggg cggcggggac
     3601 gccatgggcc agatgctcgg ccagctggga caggtgggtc agcaagtcgg gcagttggcc
     3661 caggcggccg gcgctcctct gcaggccctg gctcagccgt tgcaacaggt acctcagcag
     3721 gtgatgcagg ccgttcagca ggctagccag tcggccagta aagccgccga tgcgaccggc
     3781 gctgccaagg gcgatcccgc tgtgccccaa caggattcac gcagcgagga aactgaacgc
     3841 cgcaacgaac ccctgcatcg cgcggaggcc gccccgggag attcggcgaa taccggcagg
     3901 gcgcccgaga cgccagagca cgccaagcca gcgcagacgc gtccgcagac ggactgaatc
     3961 ggctacagcc gccggccgag atcctcgtcg ccgtggcgcg gttggtgaga cgggccaacc
     4021 ggcgcctgcc cgtgtcgtcc gtcctctgcc ccggagggcc ctgccatggc gcggtcatcg
     4081 gcgcgctgca acggctcccg ttcctcgtgt cggggttcgt gagctcgggt cggttgttcg
     4141 tctttggtgt cgacgccatc gtcgcgcggt tgcggctgac cgactgcgcc cgccgccccg
     4201 actgcacccg tgggcggagc acctgcggct gacgatccct gcccgccggc cgactgaccg
     4261 cctgccccac cgccttgggc cccctgcatg gcctgctgca tgagcgagcc gaacatgccc
     4321 gtctgcccgc cgagcgcctg cgcgggtgca cccgccatct gccccatctg tccggcttgc
     4381 tggccgacct gtccgagcat gccgacgatc tggccgaccg actgcgcgcc ggcgtcatcg
     4441 gagttctggt acgccgcctg cgcggcaccg atcttgccgg aaaacatcgt ttccttcgcc
     4501 tgcagcgtcg cgacattggc ggccagcggg acggccagtg tcggaagcac ggccgagatg
     4561 gtcgcgctca aggcgtcctg gccggcggga agcatcggta tctcgggaag ctcgacggaa
     4621 gctgggtccc atgtgatgcc ttcgggcttc ctcagcggat caatcatgac tcaccaatca
     4681 tcgtcatcgt caccttcatc gagatcgtgt tcgagcggcg tgggcaccgc gagacttgca
     4741 ctggtgccgc cactttcacc gcgatggccc atcatcggcg ccataccgcc catgccggaa
     4801 cccgcagcga ccggggccac cccgctggtt gcagtagatc cggcggtggc gccctcggct
     4861 ggcgcggtgc tcacggattt ggtgcccacc agattcgcca tctgcgccgt ctgcagtggc
     4921 gtaccgccgg agccgggcag cgacgccgcc ttcaccatgc ccgagccacc gcccgcaccc
     4981 gagccgcctg ccagcggatg gttggagaac gacgagaacg gcaggccggc ggcacccagt
     5041 gaatccgcct tgccgcctga accgaacatc gaggtcagct gttgcagcgg ctgcgacaac
     5101 tgctgcagct gctggcccat ctgctgaggc acggccatca ccgcctggcc aagctgcatg
     5161 gggatctgcg cggccatgga tcccatctgg ctcatcatct gcatcgcctg ctgggagccg
     5221 ccaccagctt gtttcgcggc ctcctccgcg gtgtctttcg tctctttccc gccgggcacc
     5281 gacggggcag caccaggaaa ggggatgctc gggggcacgc cgagagtgtt ggcaagttcg
     5341 gtcgtgtgcg tcgtcacgtt ggcgatgttc tgggacaacc ccatcttgat ccggctctgc
     5401 accagctcct gggtgttgct gaacggcatc gccgagagag cctcgcactc atgctggacc
     5461 tcctgcgcga tcgtgttcgc cagcatcttg gctttgacca cggtggcctc catcgcgcgc
     5521 agcttgcctg cgatcgcggc tgcgtgtgcg gcgttggcct cgtggccgcc gatgatcgtg
     5581 gtggcctcgc cggtggccgc cagcgacccg gcaccctccc agacgtccgc gagcttcatc
     5641 agctggccct gctgaagggg cacgatcgat ccggtgatgt tcgcggccag cgcctcatac
     5701 gtcgccgccg cggcggccaa catctcctcg tcgacgttcg gccaggcggg acccgtcacc
     5761 gtctgcgagc cgtacgggct cgaatcggcc tctatcggca tgagaaacaa atttaccgtg
     5821 ctggcgatag ctgtcggtcc gctaatcgcg tcgccgatca gggccgacta ttgcgactct
     5881 aaccggccct ccgatagccg gagccggcgc tggatcccga tcccggcgag aaaccgttcg
     5941 tcgtggctga cgacgacgaa cgaacccttg tacgcattga gcgctgcttc cagctgaccg
     6001 acgctgacca ggtccaggtt gttggtcggc tcgtcgagca gcagcaactg gggcgcgggt
     6061 tcggcgtaga gcacacatgc caacgtcgca cgcaaccgct cacctcccga caacgcggcc
     6121 accggcaggt gaattcgatt gccgctgaac aagaactgcg cgagcaggtg catacggcgg
     6181 gtgtgggtca gactcggcgc gaacgcggca agactctgcg cgacggtgcg gtcggggtcg
     6241 agcaggtcca ggcgctgcga caggtaggcg gtgcggccgt cagcgcgggc aagcgtgccg
     6301 ccgtcaggct gcaagctgcc gtcgatgacg cgcagcagtg tcgattttcc agccccgttg
     6361 tgacccgtca acgcgatgcg ttcgggaccg cggatgttca ggtcaatacc attgtttacg
     6421 aatagctttc ggccgccgtg acagatctgc aggttctcgc cccgaaatac cgtgcgcccc
     6481 gcgggcacaa tggtgtcggg cagatcgagc acgatcatgt cgtcgtcgcg aagcgcgcgt
     6541 tcggcgtcgt ccagttgggc cttcacctgg ttgacgcgtt tggcgtgtac atcgtcggac
     6601 cggcccgccg actcctgtgc gcctcgcttc atcgcgccgg cgatgatctt cggcagcccc
     6661 gcgtctttga cgttgcgtgc cgcggtgctc gaccggcgcg cagcccgctc gcgcgcctgt
     6721 tgccgttggc gcttctcgcg tttgagctgc tgctcggcgt tgcgcaggtt agcctccgcg
     6781 acgcgctgtg cctcctgcac cgactgttgg tacatagtga agttgccgcc gtaagagagc
     6841 atttcgccgc ggcgcagctc ggcgatgcgg tccattcggt cgagcagcac gcggtcgtga
     6901 ctgaccagca gcagacatcc gggaaagtca tccagcgcgt cgtaaagccg gtggcgcgcg
     6961 tcggcgtcga ggttgttggt gggctcatcg agcagcaata catccggtcg cttcaacaac
     7021 tcagcggcca agccgagcga caccacttca ccaccggaca acgagcccag tcgtcgatca
     7081 aaggtgatgt ggcccaagcc gagacggtcg agctgcgctt gggtgcgctc ctcgacgtcc
     7141 cagtcgtctc cgatcgcgac gaagacatcc tcgcctgcat cgccagctgc caacgctgtg
     7201 agcgcatcga ggaccggggc cacgcccagc gcgtcggcca cactgcggtc cgcgacgaac
     7261 ggcagcgtct gcggcagata gccgaccgtg ccgtcgacag tgattgtgcc tgcgctgggc
     7321 cggtattcgc cggcgatcag tttgagcagc gtgctcttgc ctgcgccgtt gggcgccacg
     7381 agcccggtgc ggccgtcacc gacggtgaac gacaaccccg tgaacagtgg cgtgtcgtcg
     7441 ggccaggaga aggacagatt ggaacaaacg atggaagaca tgcgagagat ctctcgtgca
     7501 tagacgactg ggaacacaaa gtccgggtct caccgcatgc gtccagggta tgcgcgccgt
     7561 caagcggtta aatatctccg cagcatgtcg gtcaccgtgc tgatctgagc cacctcgacg
     7621 cgctcgtcgg cgcggtgcgc gaaattgggg tcgccgggcc cgtagttgac cgccggaatg
     7681 ccaagcgcgg caaagcgtgc gacgtcggtc cagccgtact tggcacgcac ctgcccaccg
     7741 gcggcgtcga cgagtgcggc ggcggcgggt ttggtcagcc cgggcagcgc gccggccgcg
     7801 gagtcggtca gttcgatgtg cacgtcgagc ccgtcgaaga cctcgcggac gtggtcggtt
     7861 gcctgctcga cgctgcggtc aggagcgaag cggaaattca ccgtcaccga cgcggcatcg
     7921 gggatgacgt tgccggcgat cccgccgtcg atgcgcaccg cggacagccc ttcgcggtag
     7981 acgcagccgt cgatgtcgac gtcgcggggc tgatacgcct tcagtcgggc cagcacctcg
     8041 ccgagtttgt gaatcgcgtt gtcgcccaac caggatcgcg cactgtgcgc acgggtacct
     8101 gcagcgctga cgatgacgcg cagtgtgccc tgacaaccgg cctcgatgaa gccaccggac
     8161 ggctcgccga gaatcgcgac gtcagcatgc agccaatcgg gtagctcacg ttcgatgctg
     8221 ccgagcccgt tggcggccgc ttcgatctcc tcgcagtcgt acatcaccag cgtgaggtcg
     8281 tgtttcgggt cggcgacggt cgcggccagg tgcaggaaga ccgcgtcgcc ggacttcatg
     8341 tccgaggtgc cgcagccgtg aagcgtgtcg ccgtcgagcc tgctgggcag gttgtcggca
     8401 gcggggacgg tgtcggtgtg gccggccagc agcacccgcg acggccggcc gaggttcgtg
     8461 cgggccagca cggtgtcgcc gtggcgcacg acctcgaagc cggtggtttg ctcgcgcagc
     8521 gcggcctcga tctcgtcggc gatgcgctgc tcgtgccgcg actcgctggg gatgtccacc
     8581 agcgcagcgg tcagcgcgat cgggtcgccg cgtaagtcca ggcccacagg gttcgaggct
     8641 agtcgagtag cctttggcga cgtgacatct gctgcaggcg tcggcctggc gaccatcgct
     8701 gccgacggat ccatactcga cacgtggttc cccgagcccg aactgggcgc cggcggtccg
     8761 accggcaccg cgcggctgtc gatcgccgag attccgcctg agctggcgga gctgaccggc
     8821 catgaccacg accgcgacgt tgagacggtg gccgtgcgca cgacgatcgc gtcgctggag
     8881 gacaaggcca ccgacgcata cgacgcctac ctgcggctgc acctgctttc gcaccggctg
     8941 gtcgcgccac acgggctcaa cgcggacggc ttcttcggcg tgctcaccaa cgtggtgtgg
     9001 accaactacg gcccgtgtgc ggtggagggc ttcgaggtgg tgcgggcgag gctgcgccga
     9061 cgcggaccgg tgaccgtcta cggcatcgac aagttcccgc ggatggtcga ctacgtggtg
     9121 ccctccggcg tgcgtgtcgc agacgccgac cgggtgcggc tgggtgcgca cctggcatcg
     9181 gggacgacgg tcatgcacga gggcttcgtc aacttcaacg ccgggacgct gggcagctcg
     9241 atggtcgagg gccggatctc cgcgggcgtg gtcgtcggcg acgggtcgga cgtcggcggc
     9301 ggcgcgtcga tcatgggcac actgtcgggt ggcggcactc aggtcatctc ggtgggcaaa
     9361 cgctgcctgc tgggcgcgaa cgccgggctg ggcatttccc tcggcgacga ctgcgtcgtt
     9421 gaggccggcc tttacgtgac cgccgggacg aaggtgcaga cggccgacgg caagacggtc
     9481 aaggcccgcg agctgtcggg cgccagcaac ctgctgttcc ggcggaactc gctctccggg
     9541 gcggtggagg tcgtcaagcg cgacggcacc ggcatcacgc tcaacgaggc gctgcaccac
     9601 aactagtccc cctcgcaagc tgggggtgcc cccacgccaa ctgccgcgtg ggcccacacg
     9661 tgagtagggg acgtgcgtgt ttgcacgtag acacgccgat aatcgcgtgc attttgcgca
     9721 cactcgtcgc cgctgagcgg cgtacattct gccggtatga cccaaccgac cgtcgcactg
     9781 tttcctgaac ccggcgcgtg ggggccgacc aacaacctcg tcgccgtcgg aaatcatctg
     9841 cgggagcggg gtatccgcac ggtcttcgtc gtcgaggagt cgttcgacgg cgagctcgcc
     9901 aagcgtggct tcgaagagcg gctgatgcgg atggcgccgc cgcccgaggg cgaggagacc
     9961 gtcggcggtg gttgggccga gttcgtccgg gagacgtcgc cgcagttccg tcggccgacc
    10021 atcgagcagc tggagacggt gacggcgccc atctgggcgg aattcgttga tggcgcggag
    10081 tattcgcacg agcggctgac cgagatctgg tccgaactgc ggcccgacgc catcgtgcac
    10141 gactgcgtcg ccagcttccc atccgtgtcg ctggccggat gtccgtggat tcgcgtggtg
    10201 tccgccaatc ccctcgagat cactgacccc gacatcccgc cggtgttctc cggctacccg
    10261 tcacgcgacc gcagcggatg ggacgagttc tgggtggagt accgccgact gaccgatccg
    10321 cttgtcgagc gcgcgtcggc attccaccag caatgcggtg cgccgccatt gccggacggc
    10381 gaattccaat acgagtcacc gtatctgaac ctctacatgt atccggccga gctcgactac
    10441 cagcggtcgc ggccactcgg tgcgacgtgg catcgcatcg acacctccac ccgcacctcg
    10501 gacggcgcct tcgatgtcac cgaaaaggtc ggcagtgacg gcaaactggt gtacctgtcc
    10561 ctgggcagcc tcggctgcat ggatgtgccg ttgatgcagc gcgtgatcga ctcgctgagc
    10621 cagacctcgc accgggtgat cgtctcgatg ggtccgctgc atgaccagct caccctcggc
    10681 gaccggatgt ggggtgaaca attcgtcccg cagaccgcga tcgtgccgca ctgcgacgtg
    10741 atgatcacgc acggcggcaa caacaccgtc ggcgaggcgt tcacgttcgg ggtgccgacg
    10801 atcgtgttgc cgctgttctg ggatcagtac gacaacgcgc agcggatcga cgaatgtggc
    10861 tacggggtac ggctttccac ctacgagttc gccgacgacc agctgcatgg cgcgatcgag
    10921 cggctcagcg gcgaccggca gctggcggcg cgactcgacg ccgcgtcgaa gcgtttacag
    10981 gcctcgccgg ggcgggtggt tgccgcggac ctgatcgaaa agatcgcccg cggctgaaag
    11041 tcaatgccgt ccaacccaat ccgcgagccc agccggcgag gtgacatggt ccagtgccag
    11101 cgcgacggca ccgtgaagcg ggccgtcctc accatgctcg gcgtcgcgca ccggcggcgg
    11161 cgaacccttg cggaatgcca tcagcgcgtt gcggtatgcg tcgtcgaacg cctcgggcgc
    11221 ttcggctctc agtagggccg cgaccccgcc cagggtgacg acgtcggggt catgcacgtt
    11281 gacgtacgca ccggtacctc ggcccagcga agcggccaca gcttcaaatg ccctgcgtgt
    11341 caacttgtct cgggttggcc gatggatgag ttcgtgaacg tatgcgaccg cgtcgtcggg
    11401 gggcgcatca cctcgatgcc gggcgagtgc gcgtccgtcg acagtgagat cccagcagcc
    11461 gcgcgcaccg cacgggcatt ccagcgccgg gtcgccgaac gggacgtggc cgtattcgcc
    11521 tgcggcgccg tgcgcgccgg taagaggttc gccgttgacc actaatgcgc cgccgatacc
    11581 ttccgcgacc atcaagtaca gcgcggtcgc ggccgagcgc gcggcccctg tccgagcctc
    11641 ggccaggccg gccagcgtgg catcgttacc cagtaacagt tggacgccgg ctcgatcggg
    11701 gatcttcgcg gtcatcgccg aaaggtcgac gtcgttccat cctcgaggtg tgaactggcc
    11761 aagcctggcg ccgctgatcg gccccgcggc ggacaccgct attgccctga gccgcttggg
    11821 ccttcgccgg tagatcccgc cgatcgcatc ggccagctgc gtgaggacgg cgtacagatc
    11881 tgcctccgcg tattcggcct cggcgacgac ccgcggcaag ccgtcgatgt ccgccagagc
    11941 caagcgccag tccgtcgccc gtaagtccgc cgcaagcacg aggggcccgt cggcgtgcgg
    12001 acccagcacc gtcgtcggcc tgccgcgccc ttgagcgggc gccggcgtct cgtcaagcag
    12061 tcgcgtctgg cgcatccgcg cgaccagttc ggtggctccg ccgctgccga taccgaggcg
    12121 ctgcgcggcg accgcccgag tgatgcctgg ctcggatcgc accacaccga tgagttgaga
    12181 ggcgcccaac cagcgcatct gtgcggcttg ccctggatcc tgtgccatcg tcggtagtat
    12241 ctcacttgat taaactcgta gctcgagtat attcggccag tgtgacgtta gccgctgatc
    12301 gagttaccgg acaaggactt cgggagagtc tgcaggggct gctgtcgctc gcgctggcct
    12361 cgtgccttgc ggtgacgacc gaggtattgc ccgtggggct gctgccggat atcggcgcga
    12421 cgttcggggt gtccgagtcg gtcaccggcc tgttggtcag tttgtatgcg gtgatggttg
    12481 cgacgctcgc ggttccgctg accctcgcga cctcccgctt cgcccgcaag ccgctgctgc
    12541 agaccacctt gctctgttat gccgtgagca acgcgttggt ggccgcggcg ccggtgttcg
    12601 ccgtcgtcgg cgttggtcgg acggtcggcg ggatcacgca cgcgttgttc ttctcgttgg
    12661 tgatcggata cgccccgcga ctggtgtcgc gagcacatgt tggccgggcg ctcgcggtcg
    12721 cggcgagcgg tgcctcgacc gggatggtgc tcggtgtgcc gttgtcgacg tcactgggta
    12781 acgcgttcgg ctggcgagtg tcgttcgccg tcctggccgc gctgtcggtt ctggcgttcg
    12841 tcttgatgag caggttgctg ccgagcgtga gccacgagcc gctgtcaggt ggttccgtcg
    12901 gagcgggccg cggcgccctc gtcgcgatat ccgcatcgaa tttgttggcg tttctcgggc
    12961 aattcacggt gtacacgttt gtcatcgtgt tgctgctgtc cagtggggtc agcccggcgt
    13021 tcgtcggccc gattctgctc gcgtgcggtg ccggtggcct gctcgccgtg tggtgggccg
    13081 ggcgcaatct cgaccgaagc cccagacgag ccgcccttgt cctgctggcc atcgtgatcg
    13141 gcgcggtcgt cgcgctcggc ggcacctggc cgactctcgt ggcagtggtg gtggcaacgg
    13201 tcgtctggaa cggggcgttc gggggcttgc cgtcggtcta ccagacgtgc gcggtgcgca
    13261 cccacgccgc atcgccggaa ctggccggtg cgtgggtgaa cgcgacggcc aacgccggca
    13321 tcgccggtgg ggcggccatc ggtgccggtc tgcttcatgc ggcggggttg tcatcgctgc
    13381 cctgggtggg cgcgtcgctg atcgggctgg gcctcgccgt agtggtgctg tcccgcaagg
    13441 cattcccggc gcgggcttaa ttgctcagcc tgctgcgttg ggacgcagcg gcgggcagtt
    13501 gccctgcggg tcggcggcga gttcgaagtg ccagagctcg ttcgcataga tctgacacag
    13561 accgaactgg gttccgttgc ggatcagcca cttgtcagcc tcaacg
//
LOCUS       DASRJA010000155        17122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_165092,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000155 DASRJA010000000
VERSION     DASRJA010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17122
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165092"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VFC01_07345"
     CDS             complement(<1..762)
                     /locus_tag="VFC01_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HZN79487.1"
                     /translation="MTSPDQPDSASDVPEQFRIRQAKRERLLAEGREPYPIEVARTHT
                     LAEIRRAYPELEANTETGQIVGVAGRVMFARNSGKLCFAMLQEGDGTQLQVMVSFDRV
                     GQESLDAWKSDVDLGDIVYVHGEVISSRRGELSVLADSWQIVSKALRPLPVAHKEMSE
                     ESRVRQRYVDLIIRPEARTIARQRVAVVRAVRSALERRGFLEVETPMLQTLAGGAAAR
                     PFVTHSNALDADLYLRIAPELFLKRCLVGGFERVFE"
     gene            786..2009
                     /locus_tag="VFC01_07350"
     CDS             786..2009
                     /locus_tag="VFC01_07350"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZN79488.1"
                     /translation="MATPINAAAVAALADEVIDWRFKGLPAAWWGHAPAQICAEAPDL
                     FEGGAVGPVCVLRAEALAHNLTAMAGWCGERGVELAPHGKTHMAPQLFARQLAAGACG
                     ITAATLSHIRTYRAFGVRDVVLANELVDGAGLRWLAGELDAHPDFSLVCWVDSVRGVE
                     LMTAALSAAGAGRPVDVCVEVGMPGGRTGCRSASDVDEVASAAAASPRLRLVGVAGYE
                     AALGHDIGPDALAGVTAYLLEMRATAERLAPLFEVDRVIVTAGGSTYFDAVADVLAGD
                     WRTIVRSGAYLTHDDGLYARTSPLRAALQPALQVWAQVVSRPEPGLAVLTMGRRDVSF
                     DQDLPVPLGLADSAVTKLNDQHAYLRLGEDDRVEVGSWLGFGISHPCTVFDKWQLIPE
                     LDADDRVVGLIRTFF"
     gene            complement(2006..2821)
                     /locus_tag="VFC01_07355"
     CDS             complement(2006..2821)
                     /locus_tag="VFC01_07355"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZN79489.1"
                     /translation="MLLAIDVRNTHTVVGLISGSGNHAKVVQHWRIRTESEVTGDELA
                     LTIDGLIGDDSERLTGAAGLSTVPSVMHELREMLGQYWPSVPHVLIEPGVRTGIPLLV
                     DNPKEVGADRIVNCLAAFHKYGSAAIVVDFGSSICVDIVSAKGEFLGGAIAPGVEVSS
                     DAAAARSAALRRVELTRPRSVVGKNTVECMQAGAVFGFAGLVDGLVQRIRDDVDGFAG
                     DDVVVVATGHTAPLLLPDMRTVQHYDQHLTLDGLRLVFERNRDSQRGRLKQAR"
     gene            complement(2826..3233)
                     /gene="panD"
                     /locus_tag="VFC01_07360"
     CDS             complement(2826..3233)
                     /gene="panD"
                     /locus_tag="VFC01_07360"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HZN79490.1"
                     /translation="MLRTMLKSKIHRATVTEANLHYVGSVTIDADLMDAADLLEGEQV
                     TIVDIDNGARLVTYAITGERGSGVIGINGAAAHLVHPGDLVILIAYGTMEDAEAREYQ
                     PRVVFVDADNKQIDLGHDPAYVPADVAELMSPR"
     gene            complement(3233..4168)
                     /gene="panC"
                     /locus_tag="VFC01_07365"
     CDS             complement(3233..4168)
                     /gene="panC"
                     /locus_tag="VFC01_07365"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZN79491.1"
                     /translation="MTKPQFTAGDLNVYSGPRDVSDVTRALRASGRRVMLVPTMGAVH
                     EGHLSLIRAAKRVQGAVVVVSIFVNPLQFGAGEDLDSYPRPLDDDLAALRAEGVEIAF
                     TPTAADMYPDGRRTSVHPGPLGTELEGAARPTHFAGVLTVVLKLLQIVHPDRAFFGEK
                     DYQQLVLIRQMVADLNVDTQIVGVPIVREADGLAMSSRNRYLDEVDREQAGALSAALL
                     AGMYAASGGTAATLDAARAVLDEVPAIDVDYLEVRDPMLGPPPAEGIARLLVAARLGS
                     TRLLDNIAIDIGATAGTDGHPRVGSDESHELPWRN"
     gene            complement(4165..5091)
                     /locus_tag="VFC01_07370"
     CDS             complement(4165..5091)
                     /locus_tag="VFC01_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HZN79492.1"
                     /translation="MEHVDGLRPARLTIGIISAGRVGTALGVALERAEHVVVACSAIS
                     HASRERAQRRLPDTAVLPPDEVAGRAELLLLAVPDAELAGLVSGLAATSAVRPGTIVA
                     HTSGANGIGVLAPLTEQGCIPLAIHPAMTFTGSDEDIERLPDTCFGVTAADEVGYAIA
                     QSLVLEIGGEPFRVREDARTLYHAALAHASNHVVTVLLDAVEALRAALWGQELLGQEL
                     VGDAPGGIAERVIGPLARASLENSLQRGQAALTGPVARGDAAAVVGHLRALAEVDPQL
                     AQAYRANSLRTAQRAHAPKEVFAVFSDSSGQP"
     gene            complement(5173..6495)
                     /locus_tag="VFC01_07375"
     CDS             complement(5173..6495)
                     /locus_tag="VFC01_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6779 domain-containing protein"
                     /protein_id="HZN79493.1"
                     /translation="MADLSRGTRARRGTRRPGWLLTTVLLVLAIGASSALVFTNRVEL
                     LKLAVILALWAAVVAAFVSVIYRRQSDVDQAKVRDLKLVYDLQLDREISARREYELTV
                     ETQLRRELTSEIRAQAADEVAGLRAELAALRANLEILFDADLSHRPAIETDRSRAYGD
                     WPRTAESAGRVTASRIDTEDREDLDAKTEESPIIDVPAEPHPPEADWMTPPSYGGAHR
                     RPSETEQERRRRHRVEEEPRAWSPPSPPPQPPPPSATETTTQFTWEPPQQERPPAPPP
                     EPPPPPPRPEPEPEPVAQTPPDWQPAPAEGQWIPAGAPGSNWLSPDLSAQPTGENGSA
                     GEYVGRPRAAEPAMQAPHVEAPRGRHSAPPEAVDPGRPHAPPTLAAESGGPPPHPEPA
                     PPPEAPPAEPEERRHRRADDTGGQSVAELLARLQATPSGGGRRRRREE"
     gene            complement(6572..7048)
                     /locus_tag="VFC01_07380"
     CDS             complement(6572..7048)
                     /locus_tag="VFC01_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
                     /protein_id="HZN79494.1"
                     /translation="MGPTRKRDLTAATVLAAVVGYMLVVLLYRYFPPIDVWTGVSLLA
                     VAAAEAGWAFYVRAKINDGEIGDARGWLHPLAVARSVMIAKASAWVGALVLGWWIAVL
                     IYLLPRRSTLRVAGEDTAGAVVAAVSALALVVAALWLQHCCKSPHEPPDNADGATE"
     gene            complement(7048..7560)
                     /gene="folK"
                     /locus_tag="VFC01_07385"
     CDS             complement(7048..7560)
                     /gene="folK"
                     /locus_tag="VFC01_07385"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZN79495.1"
                     /translation="MSTVVLSIGSNLGERLAHLQSVVDGLDGAVRAVSPVYETKAWGG
                     VEQGPFLNAVLIADDPTLDCRGWLLRAQELEAAAERVRAQRWGPRTLDVDLVTCHDGP
                     REVTSRDDELTLPHPLAHLRAFVLIPWLALEPDATLTVAGEPRRIAQLLDELEPADRD
                     GVRLTELALH"
     gene            complement(7557..7952)
                     /gene="folB"
                     /locus_tag="VFC01_07390"
     CDS             complement(7557..7952)
                     /gene="folB"
                     /locus_tag="VFC01_07390"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZN79496.1"
                     /translation="MTDRIELRGLTVRGHHGVFEHERRDGQDFVVDVTVWIDLATAAA
                     TDDLADTFDYGTLAQRAADIVAGPPRKLIETVAGAIAEDVMADERVHAVEVVVHKPSA
                     PIPLTFSDVAVTARRSRRGGRGERPGLKA"
     gene            complement(7945..8763)
                     /gene="folP"
                     /locus_tag="VFC01_07395"
     CDS             complement(7945..8763)
                     /gene="folP"
                     /locus_tag="VFC01_07395"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZN79497.1"
                     /translation="MSPTRVQVMGVVNVTDDSFSDGGLFLDRDRAVEHGLALAAEGAA
                     IIDVGGESTRPGATRINPQIESSRVLPVIKALAGQGIKVSIDTMHAEVAQAALENGAH
                     IVNDVSGGRADPNMAPLLADAKVPWILMHWRSVAADRPHEAPAYHDVVTEVRDELLAS
                     VDAAVHAGVDPARLIIDPGLGFAKTAEHNWALLRALPEFVDTGIPVLVGASRKRFLGA
                     LLADSNGEARAPDGRETATAAISLLAGLHGAWGVRVHDVRSSVDALKVLEAWYD"
     gene            complement(8760..9368)
                     /gene="folE"
                     /locus_tag="VFC01_07400"
     CDS             complement(8760..9368)
                     /gene="folE"
                     /locus_tag="VFC01_07400"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZN79498.1"
                     /translation="MTRSLNNSATFTQPSFDQPRAEAAVRELLIAIGEDPNRHGLEDT
                     PARVARAYKEMFAGLYTDPDTVLDTTFDEQHDELVLVKQIPLYSTCEHHLVAFHGVAH
                     VGYIPGKDGRVTGLSKIARLVDLYAKRPQVQERLTAQIADALMRKLDPRGVIVVVEAE
                     HLCMSMRGVRKPGAVTTTSAVRGQFKCDASSRSEALDLILRK"
     gene            complement(9407..11695)
                     /gene="ftsH"
                     /locus_tag="VFC01_07405"
     CDS             complement(9407..11695)
                     /gene="ftsH"
                     /locus_tag="VFC01_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZN79499.1"
                     /translation="MNRKNVIRTLTAIAVVLLLGWSFFYFSDDTRGYKPVDTSVAMAQ
                     INSDNVKSAQIDDREQQLRLELKNANSDTDNSDKIITKYPTGYGVPLFDALNAKNTKT
                     NTVVNQGSMLGSLLIYLLPLLLLVGLFVMFSRMQTGGRMGFGFGKSRAKQLSKDMPKT
                     TFADVAGVDEAVEELYEIKDFLQNPSRYQALGAKIPKGVLLYGPPGTGKTLLARAVAG
                     EAGVPFFTISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFVDEIDAVGRQRGAGLG
                     GGHDEREQTLNQLLVEMDGFGDRQGVILIAATNRPDILDPALLRPGRFDRQIPVSNPD
                     LAGRRAVLRVHSQGKPIAPDADLDGLAKRTVGMTGADLANVINEAALLTARENGTVIT
                     GPALEEAVDRVVGGPRRKSRIISEQEKKVTAYHEGGHTLAAWAMPDIEPIYKVTILAR
                     GRTGGHALAVPEDDKGLLTRSEMIARLVFAMGGRAAEELVFREPTTGAVSDIEQATKI
                     ARAMVTEYGMSSKLGAVRYGTEHGDPFLGRTMGVQADYSHEVAQIIDDEVRKLIEAAH
                     TEAWEILTEYRDVLDVLAGELLEKETLHRAELQAIFSDVKKRPRLTMFDDFGGRVPSD
                     KPPIKTPGELAIERGEEWPKPVPEPAFKAAIAQATREAAARQTPQNGTNGNGGNGVPA
                     NLPTQPDYGAPAGWHAPGWPPQGQPPTQQHPQQGYWYPPPHWQQPYPPYQPYPQPGHP
                     APQGGAPAPNPNEDPGQDNSRT"
     gene            11915..12877
                     /locus_tag="VFC01_07410"
     CDS             11915..12877
                     /locus_tag="VFC01_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN79500.1"
                     /translation="MLTSTERLVETNGVQLRVIDAGDEDAPAVVLAHGFPELAYSWRH
                     QIPVLAEAGYRVLAPDQRGYGGSSKPEAVDAYNIVELSADIVGLLDDIGAERAVLVGH
                     DFGGVVAWGAPLLHPGRFSGVVGLSVPPVPRPKVPTTQAFHKVFGDNFFYILYFQEQG
                     PADAELNRDPATTLRKLMGSLTTTDETAAMRMLQPGPEGFIDRIPEPDGLPDWISQDE
                     FDHYVDEFTRNGFTAPLNWYRCFDRNWELTATTSAATIAVPSLFIGGGADPTLAYTPR
                     RRFSEVVSGDYREVMIDGAGHWLQQERPTEVNEALLGFLSGLEL"
     gene            12878..14068
                     /locus_tag="VFC01_07415"
     CDS             12878..14068
                     /locus_tag="VFC01_07415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN79501.1"
                     /translation="MERPLKFGVFITPFHPVGQSPTVALEYDLDRTVALDKLGYDEVW
                     FGEHHSGGYELIACPEVFIAAAAERTKHIRLGTGVVSLPYHHPLMVADRWVLLDHLTR
                     GRVIFGTGPGALPSDAYMMGIDPVEQRRMMQESLEAILALFRAAPDERIDRHSDWFTL
                     RDAQLHIRPYTWPYPEISTAAMISPSGPRLAGALGTSLLSLSMSVPGGYAALENTWGV
                     VLDQAEKSGRDEPDRADWRVLAIIHLADTRDQAIDDCTYGLQDFANYFGAAGFVPLSN
                     SVEGAQSPREFVAEYAGMGNCCIGTPGDAVAYIQDLLEQSGGFGTLLLLGHDWASPQA
                     TYHSYELFAREVMPHFKGQLTAPRASHDWAKAMRDKLLGRAGEAIVKAINEHTDELKE
                     REQT"
     gene            14068..15087
                     /locus_tag="VFC01_07420"
     CDS             14068..15087
                     /locus_tag="VFC01_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN79502.1"
                     /translation="MRASVLREGRMVLRDDVAEPAPGPGQVLVGVKACGICGSDLHFA
                     KHGADVLELNDRIEGFGGYGGMNIDLNRDVFMGHEFSAEVLEAGPDTDAPAPGTLVTS
                     LPVLVSAAGVEPIVYSNKTIGGYAERMLLSAPLLTPIPNGLDPRHAALTEPMAVGLHA
                     VNKSAIERNETALVLGCGPIGLAIIAGLRHRGLETIVAADYSARRRELAEAMGATATV
                     DPADGSPFDSTSPMVVFEAVGVPGIIDDILRRAAVGARVVVAGVCMQADTFHPYFAIA
                     KQINVQFVLAYDPTEFTDCLRAIAEGDIDVAPMITGEVGLDGVGAAFDDLAHPDAHCK
                     ILVTP"
     gene            15092..15844
                     /locus_tag="VFC01_07425"
     CDS             15092..15844
                     /locus_tag="VFC01_07425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN79503.1"
                     /translation="MSTRRPAHTDRRRAEAFGLSADDYHRYRPRYPQSLISSLLGSSH
                     LRVLDVGAGTGIASAQLTQAGAEVVAVEPDPRMARVAADNGIHVEQATFEDWQAAGRS
                     FDLVVFAQSFHWVQPERALDKVASMLRRGGRLALLSNRISPVSPTRQQLDEAYSGYLN
                     PAQRPAIDAAHDDALMAMITEHGYTIERRQVTEQLHYDTEAWVNMVFTYSNVLTLGPQ
                     ARSELRCRLEQVIGTAGVDAENDATAVICTLQ"
     gene            15921..16451
                     /locus_tag="VFC01_07430"
     CDS             15921..16451
                     /locus_tag="VFC01_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN79504.1"
                     /translation="MSQPVLDVDDYVLRPWRPADAPAVVAAYRDPEIRQWHIHSMNEN
                     EALAWIEAWPKRWRQERGCGWAAACDDRVLGQISLRTVDLDEGVAEISYWVLPAARGH
                     RIAQRSLTALTAWSFDVLGVHRIEILHSTRNPASCRVAERAGYLAEGVKRSEALHADG
                     WHDMHLHARIATDYSG"
     gene            16486..>17122
                     /locus_tag="VFC01_07435"
     CDS             16486..>17122
                     /locus_tag="VFC01_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562151.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZN79505.1"
                     /translation="MPIAARAKMSTRILLLAAAGLAAAGLSGCDASSGPTGAADKDTR
                     QVTVVGAGEVQGVPDTLTVNASIEFVAPDVSGAMNQTSDRQQAVIDALVGSGIDRKDI
                     STTQVSLQPQFAGGDSTAIVGYRASNSVDVKIRKLDTASAALALIISTGGDATRINSV
                     NYSIEDDSQLVKDARARAFNDAKDRAQQYAQLSGLDLGKVMSITESGGPTPP"
BASE COUNT         2745 a         5756 c         5719 g         2902 t
ORIGIN      
        1 ctcgaagacg cgctcgaatc cgcctaccaa gcaacgcttt aggaacagtt ccggtgcgat
       61 ccgaaggtac aggtcggcat cgagcgcatt ggagtgtgtg acgaacggac gagcggcggc
      121 accgcccgcc aaggtctgca acattggcgt ctcgacttcg agaaaaccgc gccgctccag
      181 cgcagagcgg acagcgcgca cgactgccac ccgttggcga gcaatggtgc gggcctccgg
      241 acgaatgatc aggtcgacat agcgctgccg gacacgggac tcttcgctca tctctttatg
      301 tgcaacagga agcggtcgca acgccttgga aacaatttgc caggaatcgg caagcacaga
      361 tagttccccg cgccgggaac tgatcacttc gccatgcaca tagacgatgt cgccgaggtc
      421 gacgtcggac ttccaggcgt cgagcgattc ctgcccgact cggtcgaagc tgaccatcac
      481 ctgcagctgc gtcccgtcac cctcctgcag catcgcgaag cagagctttc cggagttgcg
      541 tgcaaacatc acccgaccgg caaccccgac gatctggccc gtctcggtgt tggcctccag
      601 ctctggatag gcccgtcgga tctcggccag cgtgtgcgtg cgggccacct cgatcgggta
      661 cggctcgcgg ccctccgcaa gcagccgctc ccgcttggcc tgacggatcc ggaactgctc
      721 gggaacgtcg gacgcggagt cgggctgatc aggtgaggtc acgacgtgcc agcttaaatc
      781 aaggtatggc cactcccatc aacgccgctg cggtagccgc cttggccgac gaggtgatcg
      841 actggcgctt caagggcctg ccggctgcgt ggtggggtca cgcgcccgcg cagatctgcg
      901 ctgaagctcc cgatctgttc gagggcggcg ccgtcggacc ggtctgcgtg ctgcgcgccg
      961 aggcgctcgc acacaacctc accgcgatgg ccggctggtg cggcgagcgc ggcgtcgagc
     1021 tcgcgccgca tggcaagacg catatggctc cgcagctgtt cgcccggcag ctggccgcag
     1081 gcgcctgcgg tatcaccgcc gcgacgctga gccacatccg cacgtaccgt gcctttggcg
     1141 tgcgcgatgt ggtgctggca aacgagctgg tcgatggggc cgggttgcga tggctggcag
     1201 gcgaactgga cgcccatccc gacttctcgt tggtgtgctg ggtggattcc gtgcgcggcg
     1261 tcgagctgat gaccgcggcc ctctccgcgg cgggagcggg cagaccagtc gacgtttgcg
     1321 tcgaggtcgg gatgcccggc gggcgtaccg gctgccgttc ggcttccgat gtcgacgagg
     1381 tggcgagcgc agccgccgcg tcgccgcggc tacggctggt cggtgtcgcc ggatatgagg
     1441 cggcgctggg ccacgacatc gggcccgacg cgctggccgg cgtcacggcc tacttgctcg
     1501 agatgcgcgc cacggccgag cgactggccc cactgttcga ggttgatcgg gtgatcgtga
     1561 ccgcgggcgg cagcacctat ttcgacgctg tggccgatgt gctggctggc gattggcgca
     1621 cgatcgtgcg cagcggtgcg tatctcacgc atgacgacgg gttgtacgcg cggacgtcgc
     1681 cgcttcgcgc tgcgctgcag cccgcgctgc aggtgtgggc gcaggtggtc tcacggccag
     1741 agccggggct ggccgtgttg acgatgggcc gccgcgacgt gtccttcgac caggatctgc
     1801 cggttccgct cgggttggcc gacagcgctg tcaccaagct caacgatcag cacgcgtacc
     1861 tacggctagg cgaggacgac cgcgtcgagg tcggcagctg gctcgggttc gggatctcgc
     1921 atccgtgcac agtattcgac aagtggcaac tgatccccga gctcgatgcc gacgaccgcg
     1981 tggtcggcct gatacgcacg ttcttttagc gggcctgctt caggcggccg cgctgactgt
     2041 cgcggttgcg ctcgaacacc agccgcaggc cgtcgagcgt gaggtgctgg tcgtaatgct
     2101 ggacggtacg catatccggc agcagcagcg gtgcagtgtg tccggtcgcg accacgacga
     2161 catcgtctcc ggcgaacccg tccacgtcat cgcggatgcg ctgaaccaat ccgtcgacca
     2221 gccccgcgaa cccgaacacg gcgccggcct gcatgcattc gacggtgttc ttgccgacca
     2281 ccgaccgcgg ccgggtcaac tcgacgcggc gcagcgccgc cgagcgcgca gcggcggcgt
     2341 ccgatgagac ttcgacgccg ggagcaatgg ccccgcccag gaattcgccc ttggccgaca
     2401 ctatgtcgac gcagatcgac gagccgaagt cgacgacgat cgccgcgctg ccgtacttgt
     2461 ggaaggccgc aaggcagttc acgattcggt cggcgccaac ctctttggga ttgtcgacca
     2521 acagcgggat gcccgtacgt acccccggct cgatcagcac gtgaggcacg gacggccagt
     2581 actggcccag catttctcgt agctcgtgca tcaccgacgg aaccgtggac agcccggcag
     2641 ctccggtcag ccgctcggag tcgtcgccga tcaggccgtc gatggtcagc gccagctcgt
     2701 cgccggtcac ctcggactcg gtgcgaatac gccagtgctg caccactttt gcgtggttgc
     2761 ccgatccaga gatcagacca accacggtat gggtgttgcg gacatcgatt gcgagaagca
     2821 cgggcttacc gcggcgacat cagctcggcc acgtcggccg gcacgtaggc gggatcgtga
     2881 cccagatcga tctgcttgtt gtcagcgtcg acgaacacca cccgaggctg atactcccga
     2941 gcctcggcgt cctccatcgt gccgtaggca atcaggatca cgaggtcgcc gggatgcacg
     3001 agatgcgctg cggcgccgtt gatgccgatc acaccgctgc cgcgctcgcc ggtgatcgcg
     3061 taggtcacca gacgtgcacc gttgtcgatg tcgacgatcg tgacctgttc accctcgagc
     3121 aggtcggccg cgtccatcag atccgcatca atggtcaccg acccgacata gtgcaggttc
     3181 gcttcggtga ccgtggcacg gtgaatcttc gacttcagca ttgtccgtaa catcaattcc
     3241 tccatggtaa ttcgtggctc tcgtccgacc cgacccgtgg gtgtccgtca gtgccggcgg
     3301 ttgctccgat gtcgatagcg atgttgtcca aaagcctggt gctgcccagc cgggcagcca
     3361 cgagcaagcg ggcgatgccc tcggcaggag gcggacccaa catcgggtcg cgcacctcga
     3421 gatagtcgac gtcgatcgcg ggcacctcat ccagcacggc gcgcgcggcg tccaatgtcg
     3481 ctgcggtgcc gccggatgcg gcgtacatgc ctgccagtag cgccgccgac agggcgcccg
     3541 cctgctcgcg atcgacctca tcgaggtagc ggttgcgcga cgacatggcc agtccgtcgg
     3601 cctcgcgcac gatgggcacc ccgacgatct gcgtgtcgac gttgaggtcg gcgaccatct
     3661 gccggatcag caccaattgc tggtaatcct tttcgccgaa aaacgcgcga tccgggtgca
     3721 cgatctgcag cagcttcagc acgacggtca gcacgcccgc gaaatgcgtt gggcgggctg
     3781 ctccttcgag ttccgttccc aagggccctg ggtgcacgga ggtgcgcctg ccgtccgggt
     3841 acatgtcggc ggccgtcggc gtgaacgcga tctcaacgcc ttcggcgcgc agcgctgcga
     3901 gatcgtcgtc gagtgggcgc gggtagctgt cgaggtcttc gcccgcgccg aactgcagcg
     3961 ggttcacgaa gatcgacacc accacgacgg cgccctgcac tcgcttggcc gcgcggatca
     4021 gcgacagatg gccctcgtgc actgcgccca tcgtcggcac cagcatcacc cggcggccgc
     4081 tggctcgcag cgcccgagtg acatcggaga catcgcgcgg gcccgaatac acgttgaggt
     4141 caccggcggt gaattgcggc ttggtcatgg ctgtcccgaa gaatcggaga agacggcaaa
     4201 gacttccttg ggcgcgtgcg cgcgctgcgc ggtgcgcaat gagttggccc ggtacgcctg
     4261 cgcgagttgc ggatccacct cggcaagcgc gcgcagatga ccgacgacgg cggccgcgtc
     4321 accgcgcgca accgggccgg tcagtgccgc ctgtccgcgc tgcagcgagt tctccaacga
     4381 cgcccgcgcc agcggaccga tcacccgctc ggcgatgccg ccgggcgcgt cgccgaccaa
     4441 ttcttgaccg agcagttcct gaccccatag cgccgcccgc aatgcctcga cggcatcgag
     4501 cagcacagtg acgacgtggt tgctcgcatg ggcaagtgca gcgtggtaca gcgtcctggc
     4561 atcctcgcgt acccggaatg gctcgccacc gatctcgagc accagcgact gcgcgatcgc
     4621 atacccgacc tcgtccgcgg cggtgacgcc gaaacatgta tcgggaagtc gttcgatgtc
     4681 ctcgtccgag ccggtgaacg tcatcgcggg atggatggcc aacgggatgc aaccctgctc
     4741 ggtaagcggc gccagcactc cgatgccgtt ggcgcccgag gtgtgggcga cgatcgtgcc
     4801 cggccgcacc gccgaggtgg ccgcgagccc cgacaccagc ccggcaagct cggcgtccgg
     4861 gacggccagc agcaacaatt cggcgcggcc ggcgacctcg tcgggcggca ggacggcagt
     4921 gtcgggcagg cggcgctggg cgcgctcgcg cgacgcatgc gatatcgcgc tgcacgcgac
     4981 caccacatgc tcggcgcgct ccagcgccac accgagcgcg gtgcccaccc gaccagcaga
     5041 aatgatgcca atcgtgagcc gcgccgggcg cagtccgtcg acatgctcca tcgcagacga
     5101 cctcgcagat tgtcgtgtgt tcttcgttcc ggtcctgcgt tgcgggtacc ggacggtcgc
     5161 taagagccta actcactctt cgcggcgcct acgacggccg cctccgctcg gggtggcctg
     5221 aagcctggcc agcagctcgg caacggactg cccgccggtg tcatccgccc tgcggtgtcg
     5281 tcgctcctcc ggctcggcgg gcggtgcttc gggcggcggc gcgggctcgg gatgtggcgg
     5341 cggacccccg gactcagcgg ccaacgtcgg cggcgcatgc ggacgtcccg gatcgacggc
     5401 ctccggcggc gctgaatggc ggccccgagg cgcttcgacg tgcggtgcct gcatcgcagg
     5461 ctcggctgcc cgtggccggc cgacgtattc gcctgcagac ccgttctcgc ccgtcggctg
     5521 agccgacaaa tccggcgaca gccaattgct gcccggcgcg cccgcaggta tccactgtcc
     5581 ctcggcgggt gcgggctgcc agtccggcgg cgtctgcgca accggctccg gctcgggctc
     5641 gggacgcggt ggcggcggcg gcggttcagg gggcggggcg ggtgggcgct cctgttgcgg
     5701 tggctcccaa gtgaattgcg tcgtggtctc ggtggccgac ggaggcggcg gttgcggcgg
     5761 cggtgacggt ggtgaccacg cgcgaggctc ctcctccacg cgatgacgcc gccgcctttc
     5821 ctgctcagtc tcggacggtc tgcggtgcgc accgccgtaa ctcggcggcg tcatccaatc
     5881 cgcctcgggc ggatggggtt cggcgggcac gtcgatgatc gggctttcct cggttttcgc
     5941 gtcgaggtcc tcacggtctt ccgtgtcgat ccggctggcg gtgacccggc ccgcggattc
     6001 cgcggtgcgc ggccagtctc cgtacgcgcg gctgcggtcg gtttcgatcg cgggccgatg
     6061 cgacaggtcg gcgtcgaaca gaatctccaa attggcgcgc agcgcggcca gttcggcccg
     6121 taagcccgcg acctcgtcgg ccgcctgggc ccgtatttcg gaggtcagtt cgcggcgcag
     6181 ttgggtctcg accgtcagct cgtattcccg gcgcgccgag atctccctgt ccaactgcaa
     6241 gtcgtagacc agtttgagat cgcgcacctt ggcctggtcg acgtcgctct ggcgccggta
     6301 gataaccgac acgaaagcgg ccaccaccgc ggcccacaac gccaggatta cggcgagctt
     6361 cagcaactcg acgcggttgg tgaacaccag cgcagaactc gccccgatgg ccagcaccag
     6421 caacaccgtc gtgagcaacc aaccgggtct gcgggtgccg cgccgagcgc gcgtaccgcg
     6481 agacagatcg gccatgggcc gactgtaccc gccacaggtc atctcgcgtg tcgaccactt
     6541 cggcgattcg gccgatgccg ccgcaggtca gctactcggt ggccccgtcg gcgttgtccg
     6601 gcggctcatg tggcgacttg cagcaatgct gaagccatag cgcagcaacc accagcgcga
     6661 gcgcgctgac ggcggccacg actgcacccg cggtgtcttc gcctgccacc ctcagcgtgc
     6721 tgcggcgcgg caaaagatag atcagcacgg cgatccacca cccgagcacc agcgcgccca
     6781 cccatgccga cgccttcgcg atcatcaccg accgcgccac cgccagcgga tgcagccaac
     6841 cgcgcgcgtc accgatttcg ccgtcgttga tcttggcccg gacatagaag gcccagcccg
     6901 cctcggcggc cgcaacggcc agtagcgaca ctccggtcca cacgtcgatc ggcgggaaat
     6961 agcggtacag cagcaccaca agcatgtagc cgacgacggc ggcgagcacc gtcgcggccg
     7021 tcaggtcacg cttgcgggtc ggtcccatca gtgcagcgcc aactcggtga ggcgcacccc
     7081 gtcgcggtcg gccggctcga gttcatcgag cagctgcgca atgcgacgag gttcgccggc
     7141 gaccgtcagc gtggcatccg gttccagtgc aagccacggg atcaacacga acgcccgcag
     7201 atgcgccagc ggatggggca gcgtgagctc gtcgtcgcga gaggtcacct cgcgcggccc
     7261 gtcatggcat gtgacgagat ccacatcgag ggtgcgcggg ccccatcgct gcgcgcggac
     7321 ccgctcagcc gctgcttcca actcctgtgc ccgaagcagc catccgcggc agtcgagcgt
     7381 ggggtcatcg gcgatcagca ccgcgttgag gaacgggccc tgctcgacgc cgccccaggc
     7441 tttcgtctcg tacaccggcg ataccgcgcg cactgccccg tcgagaccat cgacgacgga
     7501 ctgcaagtgc gccaggcgtt caccgagatt cgacccgatg gacaacacga ctgtgctcat
     7561 gccttgagtc ccggtcgctc cccgcgtcca ccgcgccgtg agcgtcgcgc tgtgacagcc
     7621 acgtcggaga aggtcaacgg gatcggcgcc gacggtttgt gcacgacgac ctcgacggcg
     7681 tgcacgcgtt cgtcagccat cacgtcctcg gcgatcgcgc ccgctaccgt ctcgatgagc
     7741 ttgcgcggcg gacccgcgac gatgtcggcg gcgcgctggg cgagcgtgcc gtagtcgaat
     7801 gtgtcggcca gatcgtcggt ggcggccgcg gtcgccaggt cgatccacac cgtgacgtcg
     7861 acgacgaagt cctggccgtc gcgccgctcg tgctcgaaca cgccgtggtg cccgcgaacg
     7921 gtcaagccgc gcaactcgat tcgatcagtc ataccacgcc tcgaggacct tgagcgcatc
     7981 gactgaggag cgcacgtcgt gcacccgaac accccacgcc ccatgcaggc cggccaggag
     8041 ggagatcgcc gcggtggcgg tctcccggcc gtccggcgcg cgcgcctcgc cgttcgaatc
     8101 cgccagcagc gcaccgagga agcgtttgcg cgatgccccg accaagaccg ggatgccggt
     8161 gtcgacgaac tcgggcagcg cgcgcagcaa cgcccaattg tgttctgcgg tcttggcgaa
     8221 acccagtcct ggatcgatga tcagccgggc gggatcgacg cccgcgtgca cggcggcgtc
     8281 gacactggcc agcagctcgt cgcgcacttc ggtgaccacg tcgtggtacg ccggcgcctc
     8341 gtgaggccgg tccgcagcga ccgagcgcca atgcatcagt atccacggca ctttcgcgtc
     8401 ggccagcagc ggcgccatgt tggggtcggc gcggccgccg gacacgtcgt tgacgatgtg
     8461 ggcgccgttt tccagcgccg cctgcgcgac ctcggcgtgc atcgtgtcga tgctgacctt
     8521 aatgccttgc ccggcaagcg ctttgatcac cggaagcacg cgtgaagact cgatctgagg
     8581 attgatgcgg gtggcgccgg gacgggtcga ttcgccgccg acgtcgatga tggccgcccc
     8641 ctcggcggcc agggctaggc catgctccac ggcacggtca cggtcgagga acagtcctcc
     8701 gtcggagaag gagtcgtcgg tgacgttcac gacccccatg acctgcacgc gcgtcgggct
     8761 cactttcgca agatgagatc cagcgcctcg gaccgtgatg acgcatcgca cttgaactgg
     8821 ccacgcacgg ccgacgtggt ggtcaccgct cccggcttgc gtacgccgcg catcgacatg
     8881 cagaggtgtt cggcctcgac gaccacgatg acgccgcgcg gatcgagttt gcgcatcaac
     8941 gcgtcggcga tctgcgcggt cagccgttcc tgcacctgcg gacgtttggc gtacagatcc
     9001 accagtcgcg cgatcttcga cagcccggtc accctgccgt ccttgccggg gatgtagccg
     9061 acgtgcgcga ccccgtggaa tgccaccagg tggtgttcgc aggttgagta cagcgggatc
     9121 tgcttgacca gcaccagctc gtcgtgctgt tcgtcaaagg tggtgtccag cacggtatcc
     9181 gggtcggtgt agagcccggc gaacatctct ttgtacgccc gcgccacccg cgccggggtg
     9241 tcctccaacc cgtgtcggtt gggatcttcc ccgattgcga tcaacaactc tcgcaccgca
     9301 gcttccgcgc gaggttggtc gaatgacggt tgggtgaacg tggccgaatt gttgagcgac
     9361 cgcgtcatcg atgcctccgt tcagttatca gccatgagcc ggcggattag gtccggctgt
     9421 tgtcctgccc cggatcctcg ttcgggttcg gcgcgggtgc gccaccctgc ggggcgggat
     9481 gtccgggctg cggatagggc tgatacggcg gatacggctg ttgccagtgc ggcggcgggt
     9541 accagtaccc ctgctgcggg tgctgttgcg tcggcggctg tccctgcggc ggccagccgg
     9601 gcgcgtgcca gccggcgggc gcaccgtagt ccggctgggt cggcagattg gcaggaactc
     9661 cgttgccgcc gttgccattt gtgccgttct gcggggtttg gcgcgcagcg gcctccctgg
     9721 tggcctgtgc gatggcggcc ttgaacgccg gctcgggaac cggtttcggc cattcctcgc
     9781 cgcgttcgat ggccagctca cctggcgtct tgatcggcgg cttgtcggag ggcacgcggc
     9841 cgccgaagtc gtcgaacatg gtgagccgtg gccgcttctt cacgtcggag aagatcgctt
     9901 gcagttcggc gcgatgcagg gtttccttct ccagcagctc gccggccagg acgtcgagca
     9961 cgtcacggta ttcggtgagg atctcccacg cctcggtgtg cgcggcctcg atgagcttgc
    10021 ggacctcgtc gtcgatgatc tgcgcgacct cgtggctgta atcggcctgg acgcccatgg
    10081 tgcggccgag gaatgggtcg ccgtgctcgg tgccgtagcg gaccgcgccg agcttggagc
    10141 tcatgccgta ttcggtgacc attgcgcggg cgatcttggt ggcctgctcg atgtcggaga
    10201 ccgcgccggt ggtcggctcg cggaacacca gctcctcggc ggcgcgtccg cccattgcga
    10261 acaccagccg ggcgatcatc tcagaacggg tcaacaggcc cttgtcgtcc tcgggcaccg
    10321 cgagggcgtg cccgccggtg cgcccgcggg ccaggatcgt caccttgtag atcggctcga
    10381 tgtccggcat cgcccacgcg gccagggtgt gcccgccctc gtggtaggcg gtgaccttct
    10441 tttcctgctc gctgatgatg cgactcttgc gccggggccc gccgaccacc cggtccacgg
    10501 cctcctcgag cgccgggccg gtgatgacgg tgccgttctc ccgggcggtc aggagcgccg
    10561 cctcattgat cacgttggcc agatcggccc ccgtcatgcc gacggtgcgc ttggccagac
    10621 cgtcgaggtc ggcgtcgggc gcgatcggct tgccctgcga atgcacccgc agcaccgcgc
    10681 ggcggccggc gaggtcggga ttggacaccg ggatctggcg gtcgaagcgg ccgggccgca
    10741 gcagcgcggg gtcgaggatg tcgggccggt tggtggcggc gatcaggatg acgccctggc
    10801 ggtcgccgaa cccgtccatc tcgaccagca gctggttcag ggtctgctcg cgctcgtcgt
    10861 ggccaccgcc gaggccggcg ccgcgctggc ggccgaccgc gtcgatctcg tcgacgaaga
    10921 tgatgcaggg gctgttctgc ttggcctgct cgaacaggtc gcgcacccgg gaggcgccga
    10981 cgccgacgaa catctcgacg aagtccgaac ctgaaatcgt gaagaacgga acccccgcct
    11041 cacccgcgac ggcgcgggca agcaatgttt tgccggtgcc gggtgggccg tagagcagca
    11101 cgcccttcgg gatcttggcg cccagcgcct gataccgcga cgggttctgc aggaagtcct
    11161 tgatctcgta aagctcctcg accgcctcgt cgacacctgc cacgtcggcg aaggtggtct
    11221 tgggcatgtc cttggagagc tgtttggccc gggacttgcc gaagccgaag cccatccggc
    11281 cgccggtctg catccgggag aacatcacga acagcccgac gagcagcagc agcggcaaca
    11341 ggtagatcag cagcgagccg agcatgctgc cctggttcac gacggtgttg gttttggtgt
    11401 tcttcgcatt caacgcgtcg aacaggggca cgccgtaccc ggttgggtac ttggtgatga
    11461 tcttgtcgct gttgtcggtg tcagagttgg cgttcttcag ctcgagccgc agctgctgct
    11521 cacggtcgtc gatctgcgcg ctcttgacgt tgtcgctgtt gatctgggcc atcgccaccg
    11581 aggtgtcgac gggtttatag ccgcgggtgt cgtcgctgaa gtaaaagaac gaccagccca
    11641 gcaacagcac cacggcgatc gccgtgagcg tgcggatcac atttttccgg ttcatcaaac
    11701 atcggccgtg ctcggccagg tccttccata cgcacagctg gaaacctcaa ggctaccgct
    11761 agaccaacga atcacagttc ccggcggttc tctgcgcctt ccggcgaacg tgggttaccc
    11821 gcacgcttct tgggcgcgag gcgtgacata accccacact cgcggccaag gacgtcccct
    11881 ttccgcaacc tgccgcctgt gcttggctgt gggcgtgctc acatcgaccg aacggttagt
    11941 tgagacgaac ggggttcagc tgcgtgtcat cgatgccggt gacgaggacg ctcccgcggt
    12001 tgtcctggcc cacggtttcc ccgaactcgc gtactcctgg cggcaccaga ttccggtgtt
    12061 ggccgaggca ggctaccgcg tgctggcgcc ggatcagcgc ggctacggcg gatcgtcgaa
    12121 gccggaggcc gtcgacgcct acaacatcgt cgagctgtcc gccgacatcg tcggcctgct
    12181 cgatgacatc ggcgccgaac gtgccgtgct cgtcggccat gatttcggcg gcgtggtggc
    12241 ctggggcgcg ccgcttctgc atccgggccg gttctccggt gtggtcggat tgagcgttcc
    12301 gccggtgccc cggccgaagg tgccgacgac gcaagcgttc cacaaggtct tcggcgacaa
    12361 cttcttctac atcctctatt tccaggagca aggtccggcc gacgccgagt tgaaccgcga
    12421 cccggcgacc acgctccgca agctgatggg ctcgctgacg accaccgacg agaccgcggc
    12481 gatgcggatg ctgcagccgg gtccggaggg cttcatcgac cggatacctg agccggacgg
    12541 gctgcccgac tggatcagtc aggacgagtt cgaccactac gtggacgaat tcacccgcaa
    12601 cggtttcacc gcgccgctga actggtaccg ctgcttcgac cgcaactggg aactgaccgc
    12661 gacgacatcc gccgcgacta tcgcagtgcc gtcgttgttc atcggcggcg gcgccgatcc
    12721 gacgttggcc tatacaccgc ggcgccggtt cagtgaagtg gtctcgggtg attatcgcga
    12781 agtgatgatc gacggtgcgg ggcattggct tcagcaggag cggcccaccg aagtcaacga
    12841 ggcactgctc ggcttcctgt ccggattgga gctgtagatg gaacgtccac tgaagttcgg
    12901 tgtgttcatc acgccgttcc acccagtcgg ccaatccccc accgtcgcac tggaatacga
    12961 cctcgaccgg acggtggcgc tcgacaagct cggatacgac gaggtgtggt tcggcgagca
    13021 tcattccggt ggatacgaac tcatcgcatg tccggaggtg ttcattgccg cggccgccga
    13081 gcggaccaag cacatccggc tgggcaccgg cgtggtgtcc ctgccatatc accatccact
    13141 gatggtcgcc gatcgctggg tgctgctcga ccacctcacg cgcggacgcg tgatcttcgg
    13201 taccgggcct ggcgcgctgc cgtcggacgc ctacatgatg gggatcgacc cagtcgagca
    13261 acgccggatg atgcaggaat cgcttgaggc gattctcgcg ctgttccggg ccgcgcccga
    13321 cgagcggatc gacaggcact ctgactggtt cacgctgcgc gacgcccaac tgcacatccg
    13381 gccctacacc tggccgtatc ccgaaatatc cacggcggca atgatttccc cgtccggccc
    13441 gcgactagcc ggcgcactcg gcacatcgct gctgtcactg tccatgtcgg tgcccggcgg
    13501 ctacgccgca ttggagaaca catggggcgt ggtgctcgac caggccgaga agtccggccg
    13561 cgacgagccg gaccgagccg actggcgggt gctggctatc atccacctcg ccgacaccag
    13621 ggaccaggcg atcgacgact gcacctatgg gctgcaggat ttcgcgaact atttcggcgc
    13681 ggcgggattc gtgccgctgt cgaattctgt tgagggagcg cagtcgccgc gtgagttcgt
    13741 tgcggagtat gcgggcatgg gcaactgctg catcggcacc cccggcgacg cggtggctta
    13801 catccaggat ctgctggagc agtccggcgg gttcggcacc ctgttactgc tcggccacga
    13861 ctgggcgtcc ccgcaggcca cctaccactc ctacgaactg ttcgcccgcg aagtcatgcc
    13921 gcacttcaag ggccagctga ccgcaccgcg tgcgtcgcac gactgggcaa aggccatgcg
    13981 cgacaagctt ctcggccgcg caggcgaggc catcgtcaag gccatcaacg agcacacgga
    14041 cgaactcaag gagcgggagc aaacctgatg cgcgcatccg tcctacgtga aggtcgcatg
    14101 gtgctgcgcg acgatgtcgc cgagccggcc cccgggccgg ggcaggtact cgtcggcgtg
    14161 aaggcctgcg gaatctgcgg ttccgacctg catttcgcca agcacggcgc ggatgttctt
    14221 gagctgaacg accggatcga ggggttcggc ggctatggcg ggatgaacat cgacctgaat
    14281 cgcgacgtct tcatgggtca cgaattcagc gccgaggtgc tcgaggcggg accggacacc
    14341 gacgccccgg cgccgggcac gctcgtcaca tctctgccgg tgctggtgtc cgcagccggc
    14401 gtcgaaccga ttgtctattc caacaagacg atcggtgggt acgccgaacg gatgctgctg
    14461 tcggcgccgc tgctgacacc gatccccaac gggctggacc cgcgacacgc cgccctgacc
    14521 gaacccatgg cggtcggtct gcacgcggtg aacaagtccg cgatcgagcg caatgagacg
    14581 gcattggtgc ttggctgtgg accgatcggt ctcgcgatca tcgctggact gcgccaccgc
    14641 ggtctggaaa ccattgtggc tgccgattat tcggcccgtc ggcgcgaact cgccgaagcg
    14701 atgggcgcca ccgcgaccgt ggatccagcc gacggctcac cattcgacag cacgtccccg
    14761 atggtggtct tcgaagcggt tggcgtgccg ggcatcatcg acgacatctt gcgccgcgcg
    14821 gcggtcggcg cacgcgtcgt cgtagcaggc gtgtgtatgc aagccgacac cttccacccc
    14881 tacttcgcca tcgccaagca gatcaacgtg cagttcgtgc tggcatacga cccaaccgaa
    14941 ttcaccgatt gcttgcgggc gatcgccgag ggcgacatcg acgtcgcgcc gatgatcacc
    15001 ggcgaggtcg gcctggatgg ggtcggtgcg gcgttcgacg acctggcaca ccccgacgcg
    15061 cactgcaaga tcctggtgac tccctgacgg catgtcgacg cgacggccag cgcataccga
    15121 tcggcgtcgg gccgaggcgt tcggcctgtc tgccgacgac taccaccgct accggccgcg
    15181 ctacccgcag tccctcatca gtagcctgct tgggtccagc catctccgcg tactcgacgt
    15241 cggagcgggg acaggcatcg cgtcggcaca gttgacgcag gccggcgcgg aagtcgttgc
    15301 cgtggagccc gacccgcgaa tggcgcgggt ggccgccgac aacggcatcc acgtcgaaca
    15361 ggcgacgttc gaggactggc aagcggccgg tcggtcattc gatctcgttg tcttcgccca
    15421 gtcgttccat tgggtgcagc cagagcgcgc gctcgacaag gtcgcatcaa tgcttcgtcg
    15481 cggcggtcgc ctggcgctgc tgtcgaaccg catcagcccc gtgtccccga cacggcaaca
    15541 gctggacgag gcctactccg gttatctcaa tccggcgcag cgaccggcga tcgatgcagc
    15601 ccacgatgac gcgctgatgg cgatgatcac ggagcacgga tacaccatcg agcggcgaca
    15661 ggtcacagaa cagctgcact atgacaccga agcctgggtg aacatggtct tcacctactc
    15721 caacgtcctc acgctgggcc cgcaggcgcg ttctgagttg cgttgccgcc ttgagcaagt
    15781 catcgggacc gctggagtcg acgcagaaaa cgacgccacg gccgtgatct gcacgcttca
    15841 gtgagtcact tcggcgtcga tagtctggac gcgtgcctcg actcacgagt tccgttgtcc
    15901 cagcagggag tctcgcccga ttgtcccagc ccgtacttga cgtcgacgac tacgtactgc
    15961 ggccctggcg cccagcggat gcgcccgccg tcgtcgcggc gtatcgcgat ccggagatcc
    16021 ggcaatggca tatccactcg atgaacgaga acgaagcgct cgcctggatc gaggcgtggc
    16081 cgaagcggtg gcgacaggag aggggctgcg gctgggccgc cgcgtgcgat gatcgcgtgc
    16141 tgggtcagat cagtctgcgc acggtcgatc tcgacgaggg tgtcgcggag atctcctact
    16201 gggtgctgcc ggcggcgcgc ggccatcgta ttgctcaacg gtcgctgaca gcgctgacgg
    16261 cctggtcgtt tgatgtgctg ggggtgcacc gcatcgagat ccttcactcg acgcggaacc
    16321 cggcttcgtg ccgggtggcc gaacgagcgg gctacttggc cgagggtgtt aagcgcagcg
    16381 aagcgctgca cgccgacggc tggcacgaca tgcacctgca cgcccgtatc gcgaccgatt
    16441 acagcgggtg acgcgtctca gcgcgatcgc gatatggtgc caggcatgcc gatcgcagcg
    16501 agagcgaaga tgtccacccg aatcctcctt ctcgccgccg ccggacttgc ggccgccgga
    16561 ctttcgggat gcgacgcgtc gtccgggcca acgggtgccg cggacaagga cacccgacag
    16621 gtcaccgtcg tcggcgccgg cgaggtccag ggcgtgccgg acacacttac cgtgaatgcc
    16681 tcaattgagt tcgtcgcgcc cgacgtcagc ggcgcgatga accagaccag cgaccgtcag
    16741 caggctgtca tcgacgcgtt ggtgggctcc ggtatcgacc gcaaggacat cagcaccacc
    16801 caggtgagcc tgcagccgca gttcgccggc ggcgacagca cggcgatcgt cggctaccgc
    16861 gcgagtaatt cggtcgacgt gaagatccgc aagctggaca ctgcctcggc ggcgctggcg
    16921 ttaatcatca gcacgggcgg cgacgccacg cggatcaact cggtgaacta ttcgatcgaa
    16981 gacgattcgc aactcgtcaa ggacgcccgg gcccgggcat tcaatgacgc caaggaccgc
    17041 gcgcagcagt acgcgcagtt gtccgggctc gatcttggca aggtcatgtc gatcaccgag
    17101 tcaggcggcc cgacaccgcc aa
//
LOCUS       DASRJA010000156         5061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2987433,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000156 DASRJA010000000
VERSION     DASRJA010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2987433"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VFC01_07440"
     CDS             <1..347
                     /locus_tag="VFC01_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79506.1"
                     /translation="EPQPTPAELPTTAVVPPGFVPDPNYTWVPRTNVRRPEETETTTT
                     TTTTTTSPTETTTTSPGETTTTSPTDTTPTTTVVPGPLGPTTYTQTPTLTPTTPPTPA
                     GAPALTTPAPPQ"
     gene            394..1083
                     /gene="ftsE"
                     /locus_tag="VFC01_07445"
     CDS             394..1083
                     /gene="ftsE"
                     /locus_tag="VFC01_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HZN79507.1"
                     /translation="MITLDNVSKLYKSSARPALDNVSVKIDKGEFVFLIGPSGSGKST
                     FMRLLLAEDHPSSGDIRVSKFHVNKLAGRHIPSLRQVIGCVFQDFRLLQQKTVFENVA
                     FALEVIGKRPEVINRVVPDVLEMVGLSGKANRLPGELSGGEQQRVAIARAFVNRPLVL
                     LADEPTGNLDPETSKDIMDLLERINRTGTTVLMATHDHHIVDSMRQRVVELELGRLIR
                     DEQRGVYGMDR"
     gene            1084..1980
                     /gene="ftsX"
                     /locus_tag="VFC01_07450"
     CDS             1084..1980
                     /gene="ftsX"
                     /locus_tag="VFC01_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZN79508.1"
                     /translation="MRFGFLVNEVLTGLRRNVTMTIAMILTTAISIGLFGGGLLVVRL
                     ADQSRKIYLDRVESQVFLTNDVSANDPTCDADACKALRAKIEGRDDVKSVRFLNRDDA
                     YNDAIKKFPQYKDVAGKEAFPASFIVKLDNPEQHKDFDEAMVGQPGVLNVLNQKDLID
                     RLFAVLDGISKAAFAVALVQAVGAVLLIANMVQVAAYTRRTEIGIMRLVGATRWYTQL
                     PFLVEAMLAAFIGVVIAIVGLIVVRALFLENALNQFYQANLIAKVDYADVLYFSAPIM
                     LFLGLVMSGFTAYVTLRLYVRR"
     gene            1983..2477
                     /gene="smpB"
                     /locus_tag="VFC01_07455"
     CDS             1983..2477
                     /gene="smpB"
                     /locus_tag="VFC01_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZN79509.1"
                     /translation="MAKKADPTRSSNNRVVATNRKARHNYSILDTFEAGIQLVGTEVK
                     SLREGQASLADAFATVDDGEIWLRNLHIPEYHHGSWTNHPPRRNRKLLLHRKEIDNLI
                     GKIRDGNLTLVPLSLYFTDGKVKVELALARGKQAHDKRQDMARRDAQREVTRELGRRV
                     KGMT"
     gene            2474..3319
                     /locus_tag="VFC01_07460"
     CDS             2474..3319
                     /locus_tag="VFC01_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZN79510.1"
                     /translation="MTGVVLALLSAFGYGISDFVGGLASRRVAALRVVLVSYPIALVL
                     LTVLAAIVGGEVNQGAVFWGALCGVSQAFGIWWFYAALGAGPISVVSPLTAILVAGIP
                     VGVGLVMGERPGLIAGIGVVFALIAVVLVSREATDEDATPHKFTATVAWLTVGSGLAF
                     GLNFVLIHQAPEEAKLWPLVFARLSASVLVLVIAAVSANLRPPSGTPLRLAVLAAVLD
                     TGANIAMLLALHASLLSLAGVLMSLYPAATVLLAIVVLRERVTMWQTVGMVLALVAVA
                     MIAAG"
     gene            3412..4257
                     /locus_tag="VFC01_07465"
     CDS             3412..4257
                     /locus_tag="VFC01_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016810309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HZN79511.1"
                     /translation="MSSPSRPVTNLDKSDVLGGLFAVWDEIDTVLAGLADDQWQAQTP
                     LPGWTVHDVTAHLIGTESMLQGVATPNADIDVSTLKHVRNDVGVMNERWVRKLRGVSP
                     AELLDRFRATTAKRREELTAISDADWNQITATPAGPDTYGRFMRVRDFDCWMHLHDIR
                     DALDEPADNPAGPAARLALDEMASSMGFVVGKLGGAPDGSRVAIELSGPLARTINVAV
                     EGRGLVVDGFGDARPTATIALDALLFTRLAGGRTRLDEHADAISYSGDEAVGRRIVEH
                     LNYVI"
     gene            4337..4704
                     /gene="ssrA"
                     /locus_tag="VFC01_07470"
     tmRNA           4337..4704
                     /gene="ssrA"
                     /locus_tag="VFC01_07470"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          898 a         1609 c         1655 g          899 t
ORIGIN      
        1 tcgagcccca gccgacgcca gcggaattac cgacgactgc cgtcgtcccg cccggcttcg
       61 tacccgaccc gaactacacc tgggtaccgc ggaccaacgt gcgcaggccc gaggagaccg
      121 agaccaccac cacgaccacg acgaccacca cgagccctac cgagaccacc acgacgtcac
      181 ccggagagac cacgacgacg tcaccgacgg acaccacgcc gacgacaacg gtggtgccgg
      241 ggccgctggg accgacgacc tacacccaga cgccgaccct cacgccgacg accccgccga
      301 cgcctgcagg agcaccggcg ctgactacgc cggctccgcc gcaatagcgc tcagcggcgt
      361 ccgtccccct caccgctaca ctggcgtgcc gtgatgatca ccctcgacaa cgtgtcaaag
      421 ctgtacaagt cgtcggcgcg gccggcgctc gacaacgtct cggtcaagat cgacaagggt
      481 gagttcgtct tcctgatcgg cccgtccggt tcgggcaaat cgactttcat gcggttgttg
      541 ctcgccgagg accatccctc atcgggcgac attcgcgttt ccaagttcca cgtcaacaag
      601 ctggcgggcc ggcacatccc gagcctgcgt caggtcatcg gctgcgtgtt ccaggatttc
      661 cggctgctgc agcagaagac ggtgttcgaa aacgtcgcgt tcgcgctgga ggtcatcggc
      721 aagcgccccg aggtgatcaa ccgggtggta cccgacgtgc tggagatggt cgggctgtcc
      781 ggcaaggcca accggttgcc cggtgagctg tccggtggtg agcagcagcg ggtggctatc
      841 gcccgcgcgt tcgtcaaccg ccccttggtg ctgctggccg acgaaccgac gggcaacctc
      901 gaccccgaaa ccagcaagga catcatggat ctgctcgaac gcatcaaccg caccgggacc
      961 acggtgctga tggccactca cgaccatcac atcgtcgact cgatgcgtca gcgcgtcgtc
     1021 gaactcgagt tgggccgcct gattcgagac gagcagcgcg gcgtctacgg aatggatcgc
     1081 taagtgcgct tcggctttct cgtcaatgaa gttctgaccg ggcttcgtcg caacgtcacc
     1141 atgacgattg cgatgatcct caccaccgcc atctccatcg ggctcttcgg cggcgggttg
     1201 ctggtggtgc ggctcgccga ccagtcacgc aagatctatc tcgaccgcgt cgagagccag
     1261 gtcttcttga ccaacgacgt ctctgccaac gaccccacct gcgacgccga tgcgtgtaag
     1321 gcgctgcgcg cgaagatcga gggccgcgac gatgtgaagt cggtgcggtt cttgaaccgt
     1381 gatgacgcct acaacgacgc gatcaagaag ttccctcagt acaaggacgt ggccggcaag
     1441 gaggcgttcc cggcgtcgtt cattgtcaag ctagacaatc ccgagcagca caaggatttt
     1501 gacgaggcga tggtcggtca gcccggggtg ctcaacgtgc tgaaccaaaa ggacttgatc
     1561 gaccgattat tcgctgtgct cgacgggatc agcaaggccg cgttcgcggt ggctctggtt
     1621 caggccgtcg gtgcagtctt gttgatagcc aacatggttc aagtggcggc gtatacccgt
     1681 cgcaccgaga tcggcatcat gcgattagtg ggcgccaccc gctggtatac ccagctgccg
     1741 ttcttggtgg aggcgatgct ggccgctttc atcggcgtgg tcatcgccat tgttggtctg
     1801 atcgtggtgc gtgcgttgtt cctggagaac gcgcttaatc agttctatca ggcgaatctg
     1861 attgcgaagg ttgactacgc ggatgtgctc tacttcagcg cgccgatcat gttgttcctg
     1921 ggcctggtga tgtccggctt caccgcctac gtcacgctgc gactttacgt tcgaagatag
     1981 ccatggccaa gaaagccgac ccgacaaggt ccagcaacaa cagagtcgtc gcgacgaatc
     2041 gcaaagcgcg gcacaactat tcgatcctcg acaccttcga ggccgggatc cagttggtgg
     2101 gcaccgaggt gaagagcctg cgcgaagggc aagcgtcgct tgccgacgcg ttcgcgaccg
     2161 tcgacgacgg cgagatctgg ctgcgcaacc tgcacatccc ggagtatcac cacggcagct
     2221 ggaccaacca cccgccgcgg cgcaaccgta aactgctgtt gcaccgcaag gagattgaca
     2281 atctgattgg caagatccgg gacggcaacc tgacgctggt gccgctgtcg ctgtacttca
     2341 ccgacggcaa ggtcaaggtc gagctggcgc ttgcccgcgg caagcaggcg cacgacaagc
     2401 gccaggacat ggcgcggcgc gacgcgcagc gtgaggtgac gcgcgaactc ggccgccgcg
     2461 tcaagggcat gacctgaccg gcgtagtcct cgcgctgctc tcggcgttcg gctacggcat
     2521 cagtgacttc gtcggcggcc tcgcgtcacg gcgcgtcgcg gcgctgcggg tggtgctggt
     2581 gtcctacccg atcgcgctgg tgctgcttac ggtgttggct gcgatcgtcg gcggggaggt
     2641 caaccagggc gcagtcttct ggggtgcgct gtgcggcgtg tcgcaggcgt tcggcatctg
     2701 gtggttctat gcggcgctcg gggcgggacc gatctcggtg gtgtcgccgc tgaccgcgat
     2761 tctggtcgca ggcatccccg tcggtgtcgg cctggtaatg ggggagcggc cggggctgat
     2821 cgcggggatc ggcgtcgtgt tcgcgttgat cgcggtagtg ctggtgtcgc gggaggctac
     2881 cgatgaggac gccacgccgc acaagttcac cgcgaccgtg gcgtggctga cggtcggctc
     2941 gggtctggcg ttcggactga acttcgtgct cattcaccag gccccggagg aggccaagct
     3001 gtggccgctg gtcttcgctc gcctctctgc cagcgtgtta gtcctcgtga tcgcggctgt
     3061 gtccgccaat ctccgtcccc ccagcggcac gccgttgcgg ctagcagtgt tggccgcggt
     3121 gctcgacacc ggcgccaaca tcgcgatgct gctcgcgctg cacgcgtcac tgctgtcgct
     3181 ggccggcgtg cttatgtcgt tgtacccggc cgcgacggtg ctgctggcga tcgtcgtgct
     3241 gcgtgaacgc gtgacgatgt ggcagacggt cggaatggtg ctggccttgg tggcggtcgc
     3301 gatgatcgcc gccggctaac caccgcgagc gtgcgtgtct gcccgtcgac acgccggtgc
     3361 aggcgggcat ttcgcgcacg ttcgcgcgct gcgagcgcta cgttcggccc agtgagcagc
     3421 ccttcgcgac ccgtgacaaa cctcgacaag tccgacgtgc tgggcggcct gttcgctgtg
     3481 tgggacgaga tcgacaccgt gctcgccggc ctggccgacg accaatggca ggcgcagacc
     3541 ccgctgccgg gttggaccgt gcatgatgtg acggctcacc tgatcggcac cgagtcgatg
     3601 ctgcagggcg tcgcgacgcc caacgccgat atcgacgtgt cgacgctcaa gcacgtccgt
     3661 aacgatgtcg gcgtcatgaa cgagcgctgg gtgcgcaagc tgcggggcgt gagcccggcg
     3721 gaactgctcg accggttccg ggcaaccacg gccaaacgtc gcgaggaact cacggccata
     3781 agcgacgccg actggaacca gatcaccgcg acgcccgccg ggccggatac gtacggacgg
     3841 ttcatgcggg tgcgggactt cgactgctgg atgcatctgc acgacatccg tgacgcactc
     3901 gacgaacccg ccgacaaccc ggcgggcccg gcggcacggc tggcgctcga tgagatggcg
     3961 tccagtatgg ggttcgtggt cggcaagctc ggcggcgccc cggatgggtc gcgcgttgcc
     4021 atagagctgt ccggcccgct ggcccgcacc atcaacgtcg cggtcgaggg caggggccta
     4081 gtcgtcgacg gcttcggtga cgcaaggccg acggcgacga tcgcgctgga cgcgttgctg
     4141 ttcacccggc tggcaggcgg acggaccagg ctcgacgagc acgctgacgc catctcgtac
     4201 agcggcgacg aggccgtcgg gaggcggatc gtcgagcacc tgaactatgt gatctagctg
     4261 ggactattta atccctggct cgtgttgtag cgcccggtag aatggattgt cctgccgaaa
     4321 gtcggcgggg atgcgagggg ctgaacggtt tcgacttcgc gcatcgaatc aagggaagcg
     4381 tgccggtgca ggcaagagac caccgtaagc gtcgttgcaa ccaattaagc gccgagtcaa
     4441 atcagcgcga cttcgccctc gctgcctaag cgacggtgag tctgtcagac cgggaaagcc
     4501 ctcggcccgg accctggcat cagctagagg gatcaaccga tgagttcggt cgcgggactc
     4561 atcgggacac caacagcgac tgggatcgtc atcctgactt gttcgcgtga tcaggagatc
     4621 cgagtagagg catagcgaac tgcgcacgga gaagcctcga gggaatgccg taggacccgg
     4681 gttcgattcc cggcagctcc acatcaaagg cccaggtcag ggatgcaatc ccgagcctgg
     4741 gcctttctgc gtcaacgtct acgtccgtca ttgcaccaca tgccgacgca ccggggccgc
     4801 tttcgttgaa agtcgaacga gttagctgga tcgcggctac ccgttagtag ccatgaatag
     4861 ggctgaccca ctcgcgttgg ggttggtgat cgtcggtgca gcgacgatgg cagcagcggc
     4921 gttcttgccg cttgacgagc caacaggcgt atttcgcctt gtcagagaca acaccctaac
     4981 aacgccgaag cgaaagaccc caacgtgcga tgcatcaccc cgcagtcgca ctgctgtacg
     5041 ccgacgagcg cgttacgcgc c
//
LOCUS       DASRJA010000157         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1853763,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000157 DASRJA010000000
VERSION     DASRJA010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853763"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFC01_07475"
     CDS             <1..967
                     /locus_tag="VFC01_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZN79512.1"
                     /translation="TRLVGLTGITFANVFNACATAATAIQQTADTIRLGKYDIGIAIG
                     MDKHPRGAFTDDPAKLALPQWYAENGQFVTTKFFGMKANHYCHKHGISQATLAKVAAK
                     NFRNGEKNPNAFRRKPIPEDEILNSAVLNYPLTQYMFCAPDEGAAAVIMCRGDIAHRF
                     TDKPVYVRATEIRTRRYGAYEVHATSAPLEEDSSPTVYAAKAAYEAAGIGPEDVDVAQ
                     LQDTDAGAEVIHMAETGLCADGEQEKLIADGATEIYGSLPINTDGGLIANGEPIGASG
                     LRQVHELVRQLRGEAGDRQVPGDPRVGLAQVYGAPGTASATILSR"
     gene            1025..1486
                     /locus_tag="VFC01_07480"
     CDS             1025..1486
                     /locus_tag="VFC01_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79513.1"
                     /translation="MKFPPAPIWTRSSLAMLSATALATAGVFGIPAAAADNEPPPPPP
                     PLHNVHYTVWAEQPFNVEIYYRDVDPPNWADYSHNPYQFSPNVEADVGPNEQWNLDVG
                     LADPDQWAMVVAGTGGETPKTPNIHCALAVDGVVVKTGQGPKGALCSIRPW"
     gene            complement(1444..2082)
                     /locus_tag="VFC01_07485"
     CDS             complement(1444..2082)
                     /locus_tag="VFC01_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79514.1"
                     /translation="MASARRIGAPDAKNRGVLLDAAEQLLLEEGYAAVTSRRVAERAG
                     LKPQLVHYYFRTMEDLFLEVFRRRAEEGLGVLKTALESPQPLWALWRFSTMPEATRLT
                     MEFMGLANHRKALRAEIVYFAERFREEQNKAITKALERYGIGTADVPPVVWTVFATSV
                     SQAIVMERALGMSTGHDETFKYCEAWLRRLEGEPLPVDAYQGRIEQSAPFGP"
     gene            2257..>3198
                     /locus_tag="VFC01_07490"
     CDS             2257..>3198
                     /locus_tag="VFC01_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN79515.1"
                     /translation="MTTEYDSVDYFTDPSLVPDPHPYYDHIRAKDPVCCPINNGVIAV
                     TGWDAANTVYKDSENFSSCVAVMGPFTPMPFVPDGDDICAQLEQHRTEIPMFEHMVTM
                     DPPQHTDARSILSRLLTPKRLKENEDFMWRLADRHIDEFIADGKCEFLAAYAKPFSLL
                     VVADLLGVPQEDHADFREAFGAERPGSNIGGLDHEVIATNPLQWADEKFIRYIEERRE
                     SPRDDVLTSIATAKYPDGSTPEVVDVVRTATFLFAAGQETTAKLLGAALRVLGDRPDI
                     QNRLREDRSLIPTFIEECLRMDSPVKSVFRMARKTTTL"
BASE COUNT          633 a         1051 c          969 g          545 t
ORIGIN      
        1 cacccggctc gtcggcctga ccgggatcac cttcgccaac gtgttcaacg catgcgcgac
       61 cgcggccacc gctatccagc agaccgcgga caccattcgg ttgggcaagt acgacatcgg
      121 cattgccatc ggaatggaca aacacccgcg tggcgcattc accgacgatc ccgcgaagct
      181 cgcgctgccg cagtggtatg cagagaacgg ccagttcgtc accaccaagt tcttcggcat
      241 gaaggccaac cactactgcc ataagcacgg catctcacag gcgacgctgg ccaaggtggc
      301 cgcgaagaac ttccgcaacg gtgagaagaa cccgaatgcg ttccggcgca agccgattcc
      361 cgaggacgag atcctgaact cggccgtgct gaactacccg ctcacgcagt acatgttctg
      421 tgcaccggac gagggtgcgg cggcggtgat catgtgccgc ggcgatatcg cgcaccgatt
      481 caccgacaaa cccgtgtatg tgcgtgccac cgaaatccgc acccgccgct acggcgccta
      541 cgaggtgcac gcaacgtccg cgccgctgga agaggactcc tcaccgaccg tctacgcggc
      601 caaggccgcc tacgaggcgg cgggaatcgg tcccgaggac gtcgacgtcg cgcagttgca
      661 ggacaccgat gcgggcgcag aagtgatcca catggccgag accgggttgt gtgccgacgg
      721 agagcaggag aagttgatcg ccgacggcgc caccgagatt tatggttcgc tgccgatcaa
      781 caccgacggt gggctgatcg ccaacgggga gccgatcggc gcctcgggcc tgcgacaggt
      841 acacgaattg gtgcgccaat tgcgcggcga ggccggcgat cgacaggtcc cgggcgaccc
      901 gcgcgtcggg ctggctcagg tctacggcgc acccggcacc gcatcggcca cgatcctgag
      961 ccggtgagaa ccagatcgtc gattcgctgc ccgcgtgcgg aaacaacgtt ctaggatctc
     1021 ggaaatgaag tttccgcctg cgccgatctg gacccgctcg tcgctcgcca tgctgtcggc
     1081 aacggcgctg gcgactgccg gcgtcttcgg aatcccggca gcggccgcag acaacgaacc
     1141 accgcctccg ccgccgccgc tgcacaacgt ccactacacg gtatgggccg agcagccgtt
     1201 caacgtcgag atctactacc gcgacgtcga cccgccgaat tgggcggact acagccacaa
     1261 cccctatcag ttcagcccca acgtcgaagc cgacgtgggt ccgaacgagc agtggaacct
     1321 cgacgttggt ttggcggatc ccgatcagtg ggcgatggtc gtcgccggca ccggcggcga
     1381 gacaccgaag acacccaaca tccactgtgc gctcgccgtc gacggtgtgg tggtgaagac
     1441 cggtcaggga ccgaagggtg cgctctgttc gattcgccct tggtaagcgt cgacgggtag
     1501 cggttcaccc tcgagtcggc gaagccacgc ctcgcagtac ttgaacgtct cgtcgtgacc
     1561 ggtcgacatg ccgagcgccc gctccatcac aatcgcctgc gaaacactgg tcgcgaacac
     1621 ggtccacacc actggtggga catcggcagt accgatgccg tagcgctcga gtgctttggt
     1681 gatggccttg ttctgctcct cacggaaacg ttcggcgaaa tagacgattt ctgcccgcag
     1741 agccttgcga tggttggcca ggcccatgaa ctccatagtg aggcgggtcg cctctggcat
     1801 cgtgctgaac ctccacagcg cccacagcgg ctgcggcgac tccagtgcgg ttttcaggac
     1861 gccgagcccc tcctcggccc gacggcggaa cacctccagg aaaaggtctt ccatcgtgcg
     1921 gaagtaatag tgcacgagtt gcggcttgag gccggcgcgc tcggcgaccc gacgtgaggt
     1981 cactgcggcg tagccctcct cgagaaggag ttgttcggca gcgtcgagta gcacgccgcg
     2041 atttttcgcg tccggcgctc cgattcttcg cgccgatgcc atgttttcct cctatacctg
     2101 tgccacgcag atggtagacg ccagaccgcg gtgtcatctt gaccatgact ctacgctcat
     2161 gctaagcagg tgctcagcac atattcgtct ctcgaacgtc agccccggcg aaaaagccat
     2221 cgcgcgagct atggttttcg gaaggaagcg cacccaatga ccaccgaata cgactcagtc
     2281 gactacttca ccgatccgtc gttggtgccc gatccgcatc cgtattacga ccacatccgg
     2341 gcgaaggatc cggtgtgctg tccgatcaac aacggcgtga tcgcggtgac cgggtgggat
     2401 gcggcaaaca ccgtctacaa agacagcgag aacttctcgt cgtgtgtcgc agtcatgggt
     2461 ccgtttacgc cgatgccttt cgtccccgac ggcgacgaca tctgcgcaca actcgagcag
     2521 caccgcaccg agatcccgat gttcgagcac atggtcacga tggatccgcc gcagcacacg
     2581 gacgcacggt cgatcctttc tcgactactg accccgaagc ggctgaagga gaacgaggac
     2641 ttcatgtggc ggttggccga tcgccacatc gacgaattca tcgccgatgg caagtgcgag
     2701 ttcctcgctg catatgcgaa accgttctcg ctcttggtcg ttgccgatct actcggcgtg
     2761 cctcaagagg atcatgcgga ctttcgcgag gcgttcggag ccgaacggcc cggatcgaac
     2821 atcggcggac tcgaccatga agtcatcgcg accaacccgc tgcagtgggc agacgagaag
     2881 ttcattcggt acatcgagga gcgtcgcgag tctccccgcg acgatgtcct gacgtcgatc
     2941 gcgaccgcga agtacccgga tggctccacg cccgaggtag tcgacgtcgt ccggaccgcg
     3001 acgttcctgt tcgccgccgg gcaggaaacg accgccaagt tgctcggcgc ggcgttgcgg
     3061 gtgctcggcg accgacccga catccagaac cgactacgcg aagaccgcag cctcatcccc
     3121 accttcatcg aggagtgcct gcgcatggac agcccggtga aaagcgtatt ccgaatggcc
     3181 cgcaagacaa ccactctg
//
LOCUS       DASRJA010000158         8249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2420136,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000158 DASRJA010000000
VERSION     DASRJA010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2420136"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VFC01_07495"
     CDS             <1..793
                     /locus_tag="VFC01_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyramine oxidase"
                     /protein_id="HZN79516.1"
                     /translation="ISFFLTIGNYDYGFYWYLYLDGTIQLEAKATGIVFTSAYRGPDG
                     FATEMAPGLGAPFHQHLFSARLDMAVDGNVNVVEEVDAVPVPKGPDNPWGNAFRAQKT
                     KLTRESEAKRVGDNLKARVWHITNPTKQNRLGQDVGYALYPEGQPVLLADPSSSIAGR
                     AAFATKHLWVTKYDPAERYSAGQFVNQHPGGAGLPAFTAGDRSIDGEDIVVWHTFGLT
                     HFPRPEDWPVMPVDYAGFKLKPQGFFDRNPALDVPAAPRAHCCED"
     gene            859..1272
                     /locus_tag="VFC01_07500"
     CDS             859..1272
                     /locus_tag="VFC01_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79517.1"
                     /translation="MSTATSVTLGRRACAALALSSAGLHAAMLGHTSNVVAGGLLAAM
                     IVACVYCARDLWLRGTLRAWCVVGLMNIAMIAVHLPMPAHHHGMQMGSMGQQSTVMGL
                     ATGVAAVEVVVAMAVLYYRTRGNVVLARTPVDHLD"
     gene            complement(1240..1824)
                     /locus_tag="VFC01_07505"
     CDS             complement(1240..1824)
                     /locus_tag="VFC01_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005261618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN79518.1"
                     /translation="MDGRTLRYQHRRPELLAAATEYVLDHGIGDLSLRDMARGLGVTH
                     ATLLRHFSSKEDLLLQVVEKIRADFVDQLANDEQLRSARPASALLRATWKRLCEPAEQ
                     RQFALLFELVGRATRDGGRVAELAKSSIDDWLTVISAQLSRIGYPPKDADTLATLLLA
                     QVRGLQLDLLLTGDRRRVDRAIDALIEMVDRRAR"
     gene            1941..3098
                     /locus_tag="VFC01_07510"
     CDS             1941..3098
                     /locus_tag="VFC01_07510"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZN79519.1"
                     /translation="MADHTPSSLVELGLFTGIPQHENFSRVPELIPTSKMSPSSTPHD
                     WPATDPIGLPDTYAFNGQSRATEDFLAQTDTAALLVLHDGAISYEWYGLTGGPNVPWI
                     SMSVAKSVISALVGIAVDEDHISSIDEPIGDYIRVTPGSAYDGVSIKDVLQMSSGAGW
                     NEDYNDPASDIFRLAAAMGDGGTLEEFVATMVRDNEPGRICRYNSGDTQALGALLVGA
                     TGRSITDYMQEKLVEPLGISSPAYWLVDSAGMEVTFAGLVMTARDYAKFGELYRNGGR
                     WRGKQIVPQSWVDASLTADADHLQPGRPIVGSHPLDLGYGYQWWLPASNSGEFSAIGV
                     YNQFVYVSPTTKTVVVKLSANRMYGTSDDESTNRELETIAFLRALTGRPAR"
     gene            complement(3076..4215)
                     /locus_tag="VFC01_07515"
     CDS             complement(3076..4215)
                     /locus_tag="VFC01_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN79520.1"
                     /translation="MYDTDVLIIGAGPSGLTLAASLVKMGIATTVVDRQPAGANTSRA
                     AVVNARTLEVLDDLDVSRRLVKEGIQAPRFTIRDGRRTLIPVDFSVLPTEFPYSLMVP
                     QATTERLLLDRLTELGGTVIRPKTLTSVTQDGDGVTAAFDDGEAITARYVVGADGIHS
                     TVRKQAGIGFEGGVYEESFTLADVRLRGEAPVDEVILFWAKAGLTVVAPLPGDIYRIV
                     APVHDAPDEPSAAFIQQILDDRGLGAGRMIVTDVVWGSRFRIHHRVADSYRAGRLLLV
                     GDAAHVHSPAGGQGMNLGIQDAVALADALAAVLAGAPDTVLDDYSAARRPIAQQVVEM
                     TDRLTRLATLPRALRPIRNVAIGMAGRIPPVQRALAWRLSGLVYR"
     gene            4310..4864
                     /locus_tag="VFC01_07520"
     CDS             4310..4864
                     /locus_tag="VFC01_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN79521.1"
                     /translation="MRRSSVETKAVILAAARQRFAESGFERATIRAIAADANIDPSMV
                     MRYFGNKDQLFAAAADFDLQIPDLSDVDRKKLGSRLVAHFLDRWERDEALVVLLRSST
                     TNDEAAQRMRKIFSSQLRPVIAKLGGADAARRAGLIATQVLGLALCRYVLRLPPVVAM
                     SHEEVVAWLGPTVQRYLDAPRPAT"
     gene            complement(4857..5039)
                     /locus_tag="VFC01_07525"
     CDS             complement(4857..5039)
                     /locus_tag="VFC01_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79522.1"
                     /translation="MKLAIASAVTCVVAFVVMQLAFQNGALWLQVLGFGLFAVFLASG
                     VATIVGIAGVIAGRLK"
     gene            complement(5076..5231)
                     /locus_tag="VFC01_07530"
                     /pseudo
     CDS             complement(5076..5231)
                     /locus_tag="VFC01_07530"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
     gene            complement(5257..6171)
                     /locus_tag="VFC01_07535"
     CDS             complement(5257..6171)
                     /locus_tag="VFC01_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZN79523.1"
                     /translation="MDTPRVLLDGLAYVESPRWHDGRLWFAHWGTGEVVAVDLDGRSE
                     VVAHGPPGLGWSIDWLPDGRLLVTGPELLRQEPDGSMVVHADLSAVADQNWNEIVVDG
                     RGNIYVNGFGFDFLGGAPPEPGIIALVTPDGTARCVATEIDFPNGMVITPDNSTLIIS
                     ESFAGRLTAFDIGADGGLSNRRVWADNVGPDGICLDADGAIWTQAAATRTHTGRADAA
                     EGACVRVEEGGTVLQRIEHDRAIFAAMLGGPDRSTLFMLAAEWRGTEDVESAIAARTG
                     QVLVADAPAPGVGWPGRAGQSNSALTHR"
     gene            complement(6216..6908)
                     /locus_tag="VFC01_07540"
     CDS             complement(6216..6908)
                     /locus_tag="VFC01_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003941892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79524.1"
                     /translation="MGPVFEFAGHFWWLIFPFMGAIGGAVRAVAVANERRAQRRLERY
                     RIKQQTKVALAEASGRARTNEAGYRREMTKVLDRHDRTDARWLDYEIDIAKLLDFPLM
                     TDMRDPLTIAFHKAKSHADWLRPDSVDDIMGDRNAQLEYRDAVGEYVSAFDIAESEAI
                     RRRRSDFSAEAQERLERAQHLLRLASDDGATPQERQSAYARAQRELDGLVVLPASTRA
                     SIERKIAGEIEA"
     gene            complement(6924..7625)
                     /locus_tag="VFC01_07545"
     CDS             complement(6924..7625)
                     /locus_tag="VFC01_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN79525.1"
                     /translation="MSTTLLFVHSPVLGPTTWTYTAEVLRESGFGCAVPDLTALATMD
                     PPYYPKYATAAAAAVDERSDPVVVIGHSAAGALLPAIAEAVGDRTRGAVFVDAMLPQP
                     GRSWFDTAPPGLEAQLRGLADHGLLPPWHEWFPPGSLEALVPDQARRRRLIAEIPRLQ
                     LAYFDEPAPQARFTDSVACGFVRLGSGFDRAADKADRLGWWVARRDWDHLRMLSAPEA
                     VADLIAQAISATQDM"
     gene            complement(7622..>8249)
                     /locus_tag="VFC01_07550"
     CDS             complement(7622..>8249)
                     /locus_tag="VFC01_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZN79526.1"
                     /translation="MVLALARFGGQPAVVLGQQRAPGGLVGPAALREARRGMALAAGL
                     RLPLVLVIDTAGPALSAAAEQDGLAGEIARCLAELVTLDTPTVSVLLGQGSGGPALAM
                     VPADRVLAALHGWLAPLPPEGASAIVFRDVAHAPELAAAQGIRSADLLRHGIVDAIVP
                     ERPDAADEPMEFTQRLSATIANELHQLRAVPDGERMAARLARYRRIGL"
BASE COUNT         1381 a         2782 c         2713 g         1373 t
ORIGIN      
        1 catctcgttc ttcttgacca tcggcaacta cgactacggg ttctactggt acctgtacct
       61 cgacggcacg atccagctcg aggcgaaggc gaccggaatc gtgttcacgt cggcgtaccg
      121 cggacccgac gggttcgcca ccgagatggc gcctggactt ggcgcaccgt tccaccagca
      181 cctgttctcg gcgcggttgg acatggcggt cgacggcaac gtcaacgtcg tcgaggaggt
      241 cgacgcggtg cccgtgccga aggggcccga caatccgtgg ggcaacgcct tccgcgcgca
      301 gaagaccaag ctcacccgtg agtccgaggc gaagcgggtg ggcgacaacc tcaaggcccg
      361 ggtgtggcac atcaccaatc cgaccaagca gaaccggctg ggccaggacg tgggctacgc
      421 gctgtatccc gagggccaac cggtgctgct cgccgacccg tccagctcga tcgccggccg
      481 cgccgcgttc gcgaccaagc atctgtgggt gacgaaatac gatccggcgg aacggtattc
      541 ggcgggtcaa ttcgtcaacc agcatccggg cggtgccggt ctgccggcgt tcactgcagg
      601 ggatcgcagt atcgacggcg aggacatcgt cgtgtggcac accttcgggc tcacgcattt
      661 cccgcgcccg gaggactggc cggtgatgcc ggtggactac gcgggcttca agctcaagcc
      721 gcagggattc ttcgaccgca acccggcact ggacgttccg gctgccccgc gggcccattg
      781 ctgcgaagat tgaaaaatct cgaacggcag ggatgagttt cgtccgttcg acgtgtctgt
      841 acctacatga ggagtgagat gtccaccgcc acgtccgtca ccttggggcg acgggcgtgc
      901 gcggcgctcg cactgagttc ggcgggcctg cacgccgcga tgctgggcca cacgtcgaac
      961 gtcgtggcag gcgggctgct ggccgcgatg atcgtcgcct gtgtgtactg cgcacgcgac
     1021 ctctggctgc gcggaacgct gcgggcgtgg tgtgtcgtcg ggctgatgaa catcgcgatg
     1081 atcgcggtgc atctgcccat gccggcccat caccacggca tgcagatggg ttcgatgggg
     1141 cagcagtcga cggtgatggg tctggcaacc ggggtcgccg cggtggaggt ggtcgtcgcg
     1201 atggcggtgc tctactaccg cacccgcgga aatgtggtcc tagcgcgcac gccggtcgac
     1261 catctcgatt agcgcgtcga tcgcgcgatc cacgcggcgc cgatcgcccg tcagcagcag
     1321 atcaagctga agcccgcgca cctgcgctaa caacaaagtg gcaagggtgt ctgcgtcttt
     1381 gggcggatag ccgattcgcg acagctgcgc cgagatgacg gtgagccaat catcgatgct
     1441 cgacttcgcc agttcggcga ctcggccacc gtctcgagtc gcccgaccca ccagctcgaa
     1501 gagaagagcg aattgccgtt gttcggctgg ttcgcacagg cgtttccacg tcgcacgcaa
     1561 gagcgccgag gcgggacggg cggaacgcag ctgctcatcg ttggcgagtt gatcgacgaa
     1621 gtccgcgcgg atcttttcga cgacctgcaa cagcagatcc tccttcgacg agaagtgccg
     1681 cagcagagtt gcgtgggtga ccccgagacc gcgggccata tcccgcagcg acaagtcacc
     1741 gattccgtgg tcgagcacat attcggtagc ggcggccagt agctccggcc gtcggtgctg
     1801 gtagcggagc gtgcgtccgt ccactcggcc ttcgttcgcc ggcatatcag gccctcgctt
     1861 tcagataggc gtaccgactg gttgtgataa ccaagtggta cggctatatt aatcccatcg
     1921 tcgccgaacg agaggttgac atggccgacc acactccgtc ctcgctggtt gagctgggct
     1981 tgttcactgg aatcccgcag cacgagaatt tctcccgggt gccggagctc attcccacca
     2041 gcaaaatgtc cccgtcgtcg acgccacacg actggccggc aaccgatcca atcggccttc
     2101 cggacacgta cgcgttcaac ggacaaagcc gggcgacgga ggacttcctt gcccaaaccg
     2161 acaccgccgc gctcctggtg cttcacgacg gcgccatctc ctacgagtgg tacggactga
     2221 caggtgggcc aaacgttccg tggatctcga tgtcggtggc caagagcgtc atctcggccc
     2281 tggttggcat tgcggttgat gaggaccaca tttcgagcat cgatgaaccc atcggtgact
     2341 acatccgtgt gacgccgggc tcggcctatg acggcgtctc gatcaaggac gtgctccaga
     2401 tgtcgtcggg cgccgggtgg aacgaggact acaacgatcc ggcctccgac atctttcggc
     2461 tggctgccgc gatgggcgat ggcggaacgc tcgaggagtt cgtcgcaacg atggtgcgcg
     2521 acaacgaacc aggacgcatc tgccgctaca actccggcga tacccaagcc ctcggcgcac
     2581 tactggtcgg cgcgaccggt cgttcgatca ccgactacat gcaggagaag ctcgtcgagc
     2641 cgctgggaat cagttcgccc gcatactggt tggtcgactc ggctggcatg gaggtgacct
     2701 tcgccggcct cgtcatgacc gcccgggatt acgcgaaatt cggtgagctg taccgcaatg
     2761 gcggccgatg gcggggcaag caaattgttc cccagtcatg ggtagacgcg tcactgacgg
     2821 ccgacgccga tcacctgcag ccgggacggc cgatcgttgg cagccacccc ctcgacttgg
     2881 gatacgggta tcaatggtgg cttcctgcaa gcaattccgg tgaattcagc gcgatcggcg
     2941 tgtacaacca gttcgtctac gtaagcccaa ccacgaagac cgtcgtcgta aagctgtctg
     3001 cgaaccgcat gtacggcacc agcgacgacg agtccaccaa ccgcgaactc gagaccatcg
     3061 cctttctccg cgcgctcacc ggtagaccag cccgctgagc cgccacgcca acgcgcgctg
     3121 cacaggcgga atccgtcccg ccataccgat cgcgacattg cggatcgggc gcaaggcacg
     3181 cggcaacgtc gccaatcgcg ttagccgatc agtcatttcg acgacctgct gtgcgatcgg
     3241 gcgccgcgcg gcgctgtagt catcgaggac ggtgtcgggg gcgccggcga gcacagcggc
     3301 cagtgcgtcg gccaacgcga cggcgtcctg gatgcccaaa ttcatgccct ggccgccggc
     3361 cgggctgtgc acgtgcgctg cgtcgcccac cagcagcagg cgccccgcac ggtagctgtc
     3421 ggcgacgcgg tggtggatgc ggaagcgcga cccccagacg acgtcggtga cgatcattcg
     3481 tccggcgccg agcccacggt catcgaggat ctgctggatg aacgcggccg acggttcgtc
     3541 gggtgcatcg tgcaccggtg cgacgatccg gtagatgtcg ccgggcagcg gcgccaccac
     3601 ggtcaggccg gccttcgccc agaacaggat gacctcgtcg accggtgcct cgccgcgcaa
     3661 ccggacatcg gccagcgtga acgactcttc gtacacgccg ccttcgaagc cgatgcccgc
     3721 ttgcttgcgc acggtgctgt gaatgccgtc ggcgccaacg acgtagcgcg cggtgatcgc
     3781 ctcgccgtca tcgaatgcgg cggtcacacc gtcgccgtcc tgtgtcaccg acgtcagcgt
     3841 cttcggccgg atcaccgtgc cgccgagctc ggtcagccga tcgagcagca gtcgttccgt
     3901 ggtcgcctgc gggaccatca acgagtacgg gaattcagtc ggcagcaccg agaagtcgac
     3961 ggggatcaga gtgcgacggc cgtcgcggat ggtgaaccgc ggcgcctgta tgccctcctt
     4021 gaccagtcgg cgggacacgt cgagatcgtc gagaacctcg agcgtgcgcg cattgacgac
     4081 ggcggcgcgt gacgtgttgg ctccggcggg ctgccgatcc accacggtcg tcgcgatgcc
     4141 catcttgacc agcgacgcgg ccaatgtcag tccgctcgga cccgctccga tgatgaggac
     4201 atcggtgtcg tacatctcgt ctccttcagc tgaatgccaa cgcttgttgg cattcactat
     4261 gcgcgcggcg ccgcgctatg tcaacagttg ttggcctaca cttgttggca tgcgtcgatc
     4321 gtcagttgaa acgaaggctg tcatcctggc tgcggccagg cagcggttcg ccgagtcggg
     4381 gttcgagcga gcgacgatca gggcgatcgc ggccgacgcg aatatcgatc cgtcgatggt
     4441 gatgcgctac ttcggcaaca aggaccagct gttcgccgcg gcggccgact tcgatctgca
     4501 gatccccgat ctatcggacg tcgatcgcaa gaaacttggc tcgcgtctgg tggcacactt
     4561 tctcgatcga tgggaacgcg acgaggcgct cgtcgtgttg ttgcggtcca gtacgacgaa
     4621 tgacgaagcg gcgcaacgga tgcggaagat cttcagctcg cagttgcggc ccgttattgc
     4681 caagctcggt ggcgccgacg cggcccgccg cgccgggttg atcgccacgc aggttcttgg
     4741 gctggcgctg tgccgctacg tgctgcggct gccaccggtg gtggcgatgt cacacgagga
     4801 ggtggtggca tggctggggc cgacggttca gcgttatctc gacgccccgc gaccggctac
     4861 ttaagccttc ccgcgatcac gccggcgatg ccgacgatcg tggcgacgcc gctggccagg
     4921 aagaccgcga acaggccaaa ccccaggacc tgcagccaga gcgctccgtt ctgaaaagcc
     4981 agttgcatca caacaaacgc aacgacgcag gtaacggcgc ttgcgatcgc gagcttcacg
     5041 aaacgtcgtc tacttggggc ttgactcggc ttcggcggcg ccgcgatctg gtactgccaa
     5101 ccctgcccgt cccaatagcg cagctgaccg gttgggtctg gatacaagcc gggcggcgga
     5161 ggtggttgcg ttccctccat gcccgcaata gagccccatc ggcgtcgtgc caggtgactg
     5221 cgttgccgca tgaatggtgt tggcgcctat ggtttcctat cggtgtgtca gtgccgagtt
     5281 cgactgtccc gctcgtcccg gccatcccac accgggcgcc ggtgcgtccg cgacgagcac
     5341 ctgtccggtt cgcgcggcga tcgcgctctc cacgtcctcg gtccctcgcc attcggcggc
     5401 aagcatgaaa agggtgctgc gatcgggccc gcccagcatc gcggcgaata tcgcccggtc
     5461 gtgttcgatc cgctgcagca cagtgccgcc ttcttcgact cgtacgcagg caccctccgc
     5521 agcgtcggcg cggccggtgt gcgtgcgagt ggcggcggcc tgtgtccaga tcgcaccgtc
     5581 ggcgtcgagg cagatcccgt cgggtcccac gttgtcggcc cacacccgac ggttcgacag
     5641 tccgccgtcg gcgccgatgt cgaacgcggt cagccggccg gcgaacgact cggaaatgat
     5701 cagggtggag ttgtccggcg tgatcaccat cccgttgggg aagtcgattt cggtcgcgac
     5761 gcagcgagcg gtcccgtcgg gagtgaccag cgcgatgatc cccggttcgg gtggtgcgcc
     5821 gccgagaaag tcgaatccga agccgttgac gtagatgttg ccgcgaccgt cgaccacgat
     5881 ctcgttccag ttttggtcgg cgacggcgct caggtcggcg tgcacgacca tcgacccgtc
     5941 cggctcctgc cgcagtagct cgggaccggt gacgaggagc cggccgtccg gcagccagtc
     6001 aatcgaccag cccaagccag gcggcccgtg cgcgacgact tcgctgcgcc cgtccaggtc
     6061 gaccgcgacg acctcgcccg tcccccagtg ggcgaaccag agccggccgt cgtgccatcg
     6121 cggcgactcc acatacgcaa gtccgtctag caatacgcga ggtgtgtcca tacgcctatg
     6181 acgaccgggc gcccgtcgaa tcgacagggc ggcggtcagg cctcgatttc gcccgcgatc
     6241 ttgcgctcga tgctcgctcg cgtcgacgca ggcagcacga caagcccgtc gagttccctc
     6301 tgcgcccggg cgtacgcgct ctgccgctcc tgaggtgtcg ctccgtcgtc cgacgccagc
     6361 cgcagcagat gctgcgcacg ctccagccgc tcctgagctt cagcggagaa gtcgctgcgc
     6421 cgccgccgga tcgcctccga ctcggcgatg tcgaacgcgg acacgtactc gccgacggcg
     6481 tcgcggtatt cgagctgggc attgcggtcg cccatgatgt cgtcgacgga atcaggccgt
     6541 agccagtcgg cgtggctctt ggccttgtga aaggcgatgg tcagcggatc gcgcatgtca
     6601 gtcatcagcg ggaaatccaa cagcttggcg atgtcgatct cgtagtccag ccagcgcgca
     6661 tcggtgcgat cgtggcggtc gagcaccttc gtcatctcgc gccggtaacc cgcctcgttg
     6721 gtacgggccc gccccgacgc ctcggcgagc gcgaccttgg tctgctgctt gatccgatac
     6781 cgctcgagcc gacgctgcgc tcgccgctca ttggcgacgg ctacggctcg cacggccccg
     6841 ccgatcgcac ccatgaacgg gaagatcagc caccagaagt ggcccgcgaa ttcgaagaca
     6901 ggccccacaa ggccagaatg ccatcacatg tcttgggtcg cggaaatcgc ctgcgcaata
     6961 aggtcggcga cggcctctgg tgcgctcagc atccgcagat ggtcccagtc gcgacgggcc
     7021 acccaccagc cgagccggtc cgccttgtcg gccgcgcggt caaaccccga gccgaggcgc
     7081 acgaacccgc atgccaccga atcggtgaag cgtgcctgcg gcgcgggctc gtcgaagtag
     7141 gcgagctgca gtcgcggaat ctcggcgatg agacgtcgcc tccgtgcctg gtcagggacc
     7201 aatgcctcca gtgagcccgg cgggaaccat tcgtgccacg gcggcagcag gccgtgatcg
     7261 gcaaggccgc gcagctgagc ctccagtccg ggtggggcag tgtcgaacca gctgcgcccg
     7321 ggctgcggca gcatggcgtc gacgaacacg gcgccgcgcg tgcggtcgcc gacagcctcg
     7381 gcgatggcgg gcagcagcgc acccgctgca ctgtggccga tcacgacgac cgggtccgag
     7441 cgttcgtcga ccgccgccgc ggccgctgtg gcgtacttcg ggtagtacgg cgggtccatc
     7501 gtcgccagag ccgtcaagtc cggcacggcg caaccaaaac cgctctcccg caacacctct
     7561 gcggtatagg tccacgtcgt tgggccgagc accgggctgt gcacgaacag cagcgtcgtg
     7621 ctcacaggcc gatccgccgg taccgtgcca gccgagcggc catccgctcg ccgtcgggaa
     7681 ccgcacgcaa ctggtgcagc tcgttggcga tcgtcgccga cagccgctgc gtgaactcca
     7741 tcggctcgtc ggcggcatcg ggacgttcgg gcacgatcgc gtcgacgatg ccgtggcgca
     7801 gcaggtcggc cgatcgaatg ccttgcgccg cagccagttc cggcgcgtgc gcgacgtcgc
     7861 ggaacacgat cgcactggcc ccttccggcg gcagcggtgc cagccagccg tgcagcgcgg
     7921 ccagcacccg gtcggcgggc accatcgcca gcgccggccc gccgctgccc tggcccagca
     7981 gcaccgacac cgtcggggtg tccagggtga ccagttcggc caggcaccgg gcgatctcgc
     8041 cggccaggcc gtcctgctcc gccgcggcgg acagcgccgg tccggcggtg tcgatgacca
     8101 ggaccagcgg cagccgcagc ccggccgcca gcgccatgcc gcggcgggcc tcccgcagcg
     8161 ccgcgggacc caccagtccg cccggcgccc gctgctggcc cagcaccacc gccggttggc
     8221 cgccgaaccg ggccagcgcc agcaccatc
//
LOCUS       DASRJA010000159         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_449417,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000159 DASRJA010000000
VERSION     DASRJA010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449417"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VFC01_07555"
     CDS             <1..1059
                     /locus_tag="VFC01_07555"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN79527.1"
                     /translation="LVAECLSTPETVPPWVVNVAANVASVLEIYRFDFDAARRWQEWA
                     YPYHQRTSGPFAVLYGQCLAGIASNEELEVDEAERRFREALRLAKQCGGTGSHAARLA
                     CSLLGELLYERGDVDEAERLIAESCQLGSEGGIVEFMIARYTISARLKAMGGDCDAAR
                     EYLDDGARVAAALGLPRLRAHVDNERMRLCLPVAESPERVRSEDALPPGGVGTITAQL
                     RDETEIRGLLAGQPDQACRRAEAWVQKLQHQGRPRALLQANRLLVACLGAAGRTDEAK
                     QTLANIAAQCAELGLVRYLLDGGPELIAVLTALRDDLHSDQWSPTWPPVPSAFLDRIL
                     NAAQPISSGGAAPQARPN"
     gene            1210..>2667
                     /locus_tag="VFC01_07560"
     CDS             1210..>2667
                     /locus_tag="VFC01_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZN79528.1"
                     /translation="MKSTMQDYPLTVGAILRHGTSVHAQRQVRTLQPDGTVRASTFHQ
                     VGLRTAQLANALRDVGVTADTRVATFMWNNQEHVEAYCAIPAMGAVLHTLNIRLAAEQ
                     ISFIANHAEDEVVIVDGSLTEQLASVLPELPKVHTVIVTGNGNLAGLAETGKSVVRYD
                     DFIAGQPTGFDWPELDERCAAAMCYTSGTTGNPKGVVYSHRSTYLHSMGICAKGALEV
                     GPDDVVLPIVPMFHANAWGLIYAAMMAGADLVLPDRFLQAEPVVGLIESERPTIAGAV
                     PTVWNDILRYLEDRPDADISSLRLVGCGGSATPTHLMRTFQEKYGVYIAQAWGMTETS
                     PLATISKPAAELPEAQHWQLRSLQGRPVCGVELRIVDGEGTVLPNDGTAVGELQVRGP
                     WITGAYYGRESEESFSDGWLVTGDVGSIDPDGYVTLTDRAKDVIKSGGEWISSVQLEL
                     VISSHPAVFEAAVIAVPDDRWQERPLAAVVLDSGKE"
BASE COUNT          503 a          820 c          872 g          472 t
ORIGIN      
        1 ctagtggcgg aatgcctgtc aacgccggag actgtgccgc catgggtggt caatgtcgcg
       61 gcgaacgtcg cgtcggtcct cgagatctac cggttcgatt tcgacgccgc tcgccgatgg
      121 caggaatggg cataccccta ccaccaacga accagtggtc cgttcgcagt cctgtacggg
      181 cagtgtctcg caggaatcgc atcgaacgag gagctcgagg tcgacgaagc cgaacgtcgc
      241 ttccgcgagg cgctgcgatt ggcgaaacag tgcggaggta ccggctcgca cgccgcacgt
      301 ctcgcatgct cactcctcgg ggagctgttg tacgagcgcg gcgatgtcga cgaggctgaa
      361 cgactcatcg cggagagctg ccagctggga tcggagggtg gcatcgtcga attcatgatc
      421 gcccggtaca ccatcagcgc tcgcctgaag gccatgggag gcgactgcga tgccgccagg
      481 gagtacctgg acgacggcgc gcgcgtcgcg gcggctttag ggcttccccg gctgcgcgct
      541 catgtagaca acgagcggat gcggttgtgc ctgccggtcg ctgaatcgcc agaacgtgtc
      601 aggagcgagg atgcccttcc ccccggcgga gtgggcacga tcacagctca gcttcgcgat
      661 gaaaccgaaa ttcgcggact gctcgccggt cagccagacc aggcctgccg acgggccgaa
      721 gcctgggtgc aaaaactcca acaccaggga cgcccgcggg ccttgctgca ggcgaaccgg
      781 ctgcttgtgg cctgcctggg cgcagccggc cggaccgacg aggcaaaaca gacgctcgcg
      841 aacatcgccg cacaatgcgc cgaattgggg ctggtccgct acctcctcga cggcggccca
      901 gagctgatcg ccgtgctgac cgccctccgc gacgacctgc acagcgatca gtggagtccg
      961 acatggccgc cggtaccgtc ggcctttctc gatcgcattt tgaacgcggc ccagccgatc
     1021 agttcgggcg gggccgctcc gcaagcccgt cccaattgaa ctccgtctct gacgtgccgc
     1081 agcgcggaac agcgactcgg cgaagtaggg gtgtagccgg tgcaatgaca cgatgaagtc
     1141 aagcctgttg tcgcggcgcg atcttgactt tctgctgttt gagtggcggt ggggcggaag
     1201 ggtcggaacg tgaaaagcac gatgcaggac taccccttga cggtcggcgc gatcctccga
     1261 cacggaacga gtgtgcatgc gcagcgacag gtccgaacgc tccagcccga cggcaccgtg
     1321 cgagcctcga cctttcacca agtcggcctg cggacagcgc agctggccaa cgcgttacgc
     1381 gatgttggag taaccgccga cacgcgagtg gccaccttca tgtggaacaa ccaggaacac
     1441 gtcgaggcct actgcgccat tccagccatg ggagcggtgc tgcacacact caacatccgc
     1501 ttggctgcag agcaaatcag cttcatcgca aaccacgccg aggacgaagt cgtcatcgtc
     1561 gacggcagcc tgacggagca actggcctct gttttgcccg agcttccgaa ggtgcacacc
     1621 gtcattgtga ccggcaatgg aaatttggcg ggtttggccg agaccgggaa atcggttgtg
     1681 cgctacgacg atttcatcgc tggccagccg actggctttg actggccgga gctcgatgaa
     1741 cgctgcgccg ccgcaatgtg ttacacgtca ggcacaacgg gcaatcccaa aggtgttgtg
     1801 tatagccacc gatcgacata cctgcactca atgggcatct gcgccaaggg cgcactcgag
     1861 gtcggacccg acgatgtcgt gctgcccatc gtaccgatgt tccatgcgaa cgcctggggg
     1921 ttgatctacg ccgccatgat ggccggcgcc gacttggtgc taccggaccg ctttctgcaa
     1981 gcagaacccg tggtcggact cattgagagc gagcggccga cgatcgccgg tgccgtcccc
     2041 actgtatgga acgacatcct gcgatacctc gaggaccggc ctgacgcgga tatctcgtca
     2101 ttgcgtctgg tcggctgtgg tggttcagcg actccgacgc acctgatgcg gaccttccag
     2161 gagaagtacg gggtgtacat cgcccaggcg tgggggatga cggagacctc accgctggcc
     2221 acgatctcca agccggctgc cgaactgccg gaggcacagc attggcaact gcgctctctc
     2281 caagggcggc ccgtgtgcgg cgtcgagtta cgaatcgtcg acggtgaggg aacggtgctg
     2341 ccgaatgatg gaactgcggt cggcgaattg caggtgcgag gtccatggat caccggtgcc
     2401 tactacgggc gggaatcgga ggagtcgttc agcgatggct ggctggtcac cggcgatgtt
     2461 ggttcgattg atcccgacgg gtatgtcacg ctgactgatc gcgccaagga cgtcatcaaa
     2521 tccgggggcg agtggatctc ctcggtacaa ctggagctcg tgatctcctc acatccagcg
     2581 gttttcgagg ctgcggtcat cgccgtgccc gacgacagat ggcaggaacg tccgcttgcc
     2641 gcggtcgtct tggactctgg taaggag
//
LOCUS       DASRJA010000160        16323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3829068,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000160 DASRJA010000000
VERSION     DASRJA010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16323
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3829068"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..2053)
                     /locus_tag="VFC01_07565"
     CDS             complement(14..2053)
                     /locus_tag="VFC01_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZN79529.1"
                     /translation="MTLQDTESADGAPEAEANGSKSARVGESEAPFTVTAPVPYTGSG
                     ALRNPFPPIADYAFLSDCENTCLISSAGSVEWLCVPRPDSPSVFGAILDRGAGHFRLS
                     PYGVSVPAARRYLPGSLILETTWQTHTGWMIVRDTLVMGPWHDIETRSRTHRRTPMDW
                     DAEHILLRTVRCVSGTVELVMNCEPAFDYHRIPATWEYSAQAYGEAIARASRDPDAHP
                     TLRLTTNLRLGLEGHEARARTRMKEGDNAFVALSWSKHPAPQTYDEAADKMWQTSECW
                     RQWINIGDFPDHPWRSYLQRSALTLKGLTYSPTGALLAAPTTSMPETPQGERNWDYRY
                     AWVRDSTFALWGLYTLGLDREADDFFAFIADVSGANNGERHPLQVMYAVGGERSLVEE
                     ELHHLSGYDNARPVRIGNGAYNQMQHDIWGTMLDSVYLHAKSREQISDTLWPVLKQQV
                     EEAIKHWKEPDRGIWEVRGEPQHFTSSKIMCWVALDRGSKLAELQGEKSYAQQWRAIA
                     EEIKADVLANGVDSRGVLTQRYGDDALDASLLLAILTRFLPADDPRVRATVLAIADEL
                     TEEGLVLRYRVEETDDGLSGEEGTFTICSFWLVSALVEIGEIHRAKHLCERLLSFASP
                     LHLYAEEIEPRTGRHLGNFPQAFTHLALINAVVHVIRAEEEADSSGVFVPANAPM"
     gene            complement(2122..3342)
                     /locus_tag="VFC01_07570"
     CDS             complement(2122..3342)
                     /locus_tag="VFC01_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN79530.1"
                     /translation="MPAADRTTCVIAGGGPAGIMLGLLLARGGVDVTVMEKHADFLRD
                     FRGDTVHASTLRLLDELGMADDFARMPHRVIDSISMKIQGTEVAIDLRRIPGPHKHIA
                     LVPQWDLLELLAAAGNKEPTFRLLRSTEVLAPIRRGDKVVGVSYRDIDGQVREMHAAL
                     TVACDGRSSTLRDAMGLTPRSFGAPMDVWWFRLPRNPDDPHGLAGVLGAGHALIVIDR
                     GDYFQCAYVIPKGRDVELRAQGIETLHRGVVALVPWLADHVKAVTSWDDVKLLDVQLN
                     RLRRWYTDGLLLIGDAAHAMSPVGGVGINLAVADAVAAAGALAAPLRRGTVSTRQLAR
                     VQARRWLPAALIQSVQRVIHSRVIAVAVSAGQGAEPPLFVRIANKVPALRTAAGYGVA
                     IGPLPEHVPEFARR"
     gene            3375..3569
                     /locus_tag="VFC01_07575"
     CDS             3375..3569
                     /locus_tag="VFC01_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79531.1"
                     /translation="MGGFLNWWDGVELWLSGLPFVAQTAVVMPVVLALAYGIAILLDG
                     ALGNGIRLLRRVRHQNEDEE"
     gene            3917..4945
                     /locus_tag="VFC01_07580"
     CDS             3917..4945
                     /locus_tag="VFC01_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZN79532.1"
                     /translation="MLNIRKSWRAAAVLTATATVLAACGGGSSDVAGGGDQPAAETTL
                     TLVAYAVPEPGWSKIIPAFAATPEGKGVAVTTSYGASGDQSRAVVDGKPADIVNFSVE
                     PDVTRLVKADKVAKDWNADATKGIPFGSVVSLVVRKGNPKNIKDWDDLLQPGIEVVTP
                     SPLSSGSAKWNLLAPYAAKSNGGKDQQAGLDFVNKLVTEHVKTRPGSGREATDVFLQG
                     TGDVLISYENEAINVERQGKPVEHINPPQTFKIENPVAVVTSSTHLDKATALKNYLFT
                     AEGQKIWAQAGFRPVDPSVATDFATDFPTPQKLWTIADLGGWSAVDPALFDKENGSIT
                     KIYKQATG"
     gene            4942..5826
                     /gene="cysT"
                     /locus_tag="VFC01_07585"
     CDS             4942..5826
                     /gene="cysT"
                     /locus_tag="VFC01_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HZN79533.1"
                     /translation="MTATVEPNPEATRPELTDNGEGGRAPRLLGGRYGTTSLRVGAAS
                     IWLSVIVLLPLAAILWQSAGGGWDAFWLAVTSNAAVESFRVTLTISAGVTLVNVVFGL
                     LVAWVLTRDDFPGKRLVDSVIDLPFALPTIVASLVMLALYGPNSPVDLHLQHTKWGVG
                     VALLFVTLPFVVRSVQPVLLELDRETEEAAASLGANNRTIFTQVILPALLPSLLSGAG
                     LAFSRAIGEFGSVVLIGGAVPGETEVSSQWIRTLIENDDRTGAAAISIVLLVISFVVL
                     FILRAIGSRAAKREELAP"
     gene            5823..6641
                     /gene="cysW"
                     /locus_tag="VFC01_07590"
     CDS             5823..6641
                     /gene="cysW"
                     /locus_tag="VFC01_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HZN79534.1"
                     /translation="MTLSPVVRHLLRYLALAYIVILVIVPVGLILWRTFTPGVGAFVA
                     SITTPAALSALQLSLLVVAIVVPLNVLFGVPTALVLARNRFRGKSALQAVIDLPFAVS
                     PVVVGVALILLWGSAGLFGFVENNLGFKIIFGFPGIVLASIFVTVPFVIREVEPVLHE
                     IGTDQEEAASTLGSQWWQTFWRVTLPSIRWGLTYGVVLTIARTLGEFGAVIMVSSNLP
                     GTSQTLTLLVADRYSRGAEYGAYAVSTLLMTVAVIVLIVQVVLDARRSRASKQV"
     gene            6657..7715
                     /locus_tag="VFC01_07595"
     CDS             6657..7715
                     /locus_tag="VFC01_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HZN79535.1"
                     /translation="MTDAAIIVRGANKRYGDFAALDNVDFEVPSGSLTALLGPSGSGK
                     STLLRAIAGLDLPDTGTITIDGHDVTHIPPQRRGIGFVFQHYAAFKHLTVRDNVAFGL
                     KIRKRPKAEIKEKVDNLLEVVGLAGFQTRYPNQLSGGQRQRMALARALAVDPQVLLLD
                     EPFGALDAKVREDLRAWLRRLHDEVHVTTVLVTHDQAEALDVADRIAVLNKGRIEQVG
                     SPTDVYDTPANAFVMSFLGAVSSLNGSLVRPHDIRVGRNPDMAIAASDDSVQTTGVVR
                     ATIDRIVVLGFEVRVELTSAADHTPFTAQITRGDAEALGLKEGDTVYVRATRVPALPG
                     GTELPRADKADEGELTTA"
     gene            complement(7721..8737)
                     /locus_tag="VFC01_07600"
     CDS             complement(7721..8737)
                     /locus_tag="VFC01_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN79536.1"
                     /translation="MTTKVLRARVSRFGGPDVLEIDEVTPARPSRKKVRVRVTHASVG
                     STDAMARSGDYLLQPRPGFVPGYDFVGVLETVDTAADRRGLRPGMRVTGCLARMGSYI
                     TELVLPSNRLVPVPDSLDSAQVAALPLDLVTAALCIELGRAPSGGTIFVQGVSGSVGA
                     LVSQYGSANDVKVVGTASERTRGFAESFGAHVVDYHDPNWPVQVRELTSGGADAGIDH
                     TGGPAARAAIAPHGTLVRTAWSGRPGHGRRDAAVGGLFANLRRYASPRERVCSVPLLV
                     ALQPEKYRQILTDQLNRIADGALRGPAVTTVPFTEVVGAHTDIGTQPPGHKLVLQMPG
                     SVDS"
     gene            8764..9342
                     /locus_tag="VFC01_07605"
     CDS             8764..9342
                     /locus_tag="VFC01_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN79537.1"
                     /translation="MSGKTGRPRDTSLDAAIAAATESVLLAEGYAAVSIDRVAKIAGT
                     TRAAVYRRVKSRAELVVGLLIHRFGTSPAPDTGDLRQDIEHVQAVQRIFFAEAIVRAG
                     LAGLLSDTYADPTLGAALYDRFLAPRRHSVAAMLARAADRGQITPVDDPARISDILTG
                     PLLYRTLVPAIGQIDDALVEATVNAALSAVSS"
     gene            complement(9339..10061)
                     /locus_tag="VFC01_07610"
     CDS             complement(9339..10061)
                     /locus_tag="VFC01_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HZN79538.1"
                     /translation="MRVTRFVTSLVLTAHGSADPRSAASAHEVAHTIRMIRRDVDVRV
                     AFCEQNFPDLRDVLAATGSNAVVVPLLLADAYHARVDIPALIAESGANAIQADVLGED
                     DRLVAVLRQRLTHAGVSQLDPDVGVLVTAVGSSRPQANARTSLVADYLVQHTRWTATT
                     AFATGPHPTLAEATDILRERGATRLVIAPWFLAHGRITDRVTNFARAQRIPMAAPLGP
                     HRLVADTVLDRFDQALAERVAA"
     gene            complement(10040..10744)
                     /locus_tag="VFC01_07615"
     CDS             complement(10040..10744)
                     /locus_tag="VFC01_07615"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZN79539.1"
                     /translation="MTDLISDSDLQALAEGGAAELEGASALDLLRWTDENFGGNYIVA
                     SNMQDAVLVDLAAKVRPGVDVLFLDTGYHFVETIGTRDAVEAVYDINVVNVTPENSVE
                     KQNGLFGKDLFAREPNECCRMRKVEPLSKALRGYSAWVTGIRRVEAPTRANAPLISWD
                     KAFGLVKINPLAAWTDEDMQNYIDANDVLVNPLVFEGYPSIGCAPCTAKPVEGADPRS
                     GRWAGTSKTECGLHAS"
     gene            complement(10741..12411)
                     /locus_tag="VFC01_07620"
     CDS             complement(10741..12411)
                     /locus_tag="VFC01_07620"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HZN79540.1"
                     /translation="MTTAKPVKRPRGEGQWKLGYREPLNPNEQFKKDDDALNVRARIL
                     DIYSKRGFDSIDKQDLRGRMRWMGLYTQREQGYDGTWTGDENADVLEARYFMMRVRCD
                     GGALTTQALRTLGEISTEFARDTADISDRENVQYHWIQIEDVPEIWQRLAAVGLQTTE
                     ACGDCPRVVLGSPLAGESVDEVLDPTWAIDEIVRRYIGSPELSNLPRKFKTAISGLQD
                     VAHEVNDVAFIGVNHPEHGPGLDLWVGGGLSTNPMLAQRVGAWVPLEEVPDVWEAVTS
                     VFRDYGYRRLRAKARLKFLIKDWGIEKFRQVLEDEYLGRKLIDGPAPEPVKHPIDHVG
                     VQKLKNGKNAVGVAPIAGRVTGTILTKVADLAEAAGSDRVRFTPYQKLVILDVADEKL
                     DELVAGLDALGLPSQPSAWRKNLMACTGIEYCKLSFAETRNRAQVLVPELEKRLEDIN
                     AQLDVPITVNINGCPNSCARIQVADIGFKGQMVDDGHGGSVEGFQVHLGGSLGLDSGF
                     GRKLRQHKVLSTELGDYIDRVVRNFVKQREQGERFATWAVRADEADLR"
     gene            12705..13880
                     /gene="hemW"
                     /locus_tag="VFC01_07625"
     CDS             12705..13880
                     /gene="hemW"
                     /locus_tag="VFC01_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZN79541.1"
                     /translation="MTVRTAPLQLPDLATAPGRPFGIYIHVPFCATRCGYCDFNTYTP
                     AELGGANPDGWLEALRVELALAASRLGSPPEVDTVFVGGGTPSLLGGDGLRAVLDAIR
                     AHFTLAADVEVTTEANPESTSPRFFEQLRSAGFTRVSLGMQSVASHVLAVLDRVHSPG
                     RALSAAVEARGAGFEHINLDLIYGTPGESDDDLRRSVDAAIDAGVDHVSGYALVVEDG
                     TALARRVRRGEIAAPDNDVLAQRYELLDERLSAAGFDWYEVSNWSRPGGECRHNLGYW
                     NGGEWWGAGPGAHGYVGTTRWWNVKHPNAYAQSLADGLLPVADFEQLDATALHVEDVM
                     LRLRLRDGLPVAVLGDEERARAETVIGDGLVELHADRLVLTDRGRLLADAAVRTLLD"
     gene            14031..14546
                     /locus_tag="VFC01_07630"
     CDS             14031..14546
                     /locus_tag="VFC01_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN79542.1"
                     /translation="MTDDSEQAALLRAVHDEHGQSLLRYVLRLTRGDMPFAEDVVQES
                     LLRLWRKPEVLQQPTDNVRAWLFTVARNLVIDDRRSARYTRELQSEYLPERPSPDAIG
                     PAFDKWILSDALTSLSPEHRTAIVRAYYLGQTVADIAQQEGIPEGTVKSRLHYALRAL
                     RIALQERGVVR"
     gene            14543..15250
                     /locus_tag="VFC01_07635"
     CDS             14543..15250
                     /locus_tag="VFC01_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79543.1"
                     /translation="MTSYPPEDNLAEWDAAYVLGALSLEERRTYENYLAANPARAAEL
                     TELAGMPGILNALSRDEAVALTDLAGAPPVESRPDNVASLAQAAAKRQQRSRRTWLAA
                     AVGSAAALLIAGGIVGSTVFPQSSTPTQTVAMQAMQPTPRGGLTAELAVTEKKWGTEL
                     NWACQYTKDWSRNVDSYDIVVTTHDGVQQTVGSWKPAGDEATGLSAATSIPTKQIRTV
                     DIRVTGSNEPLAIKTLR"
     gene            15518..>16323
                     /locus_tag="VFC01_07640"
     CDS             15518..>16323
                     /locus_tag="VFC01_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN79544.1"
                     /translation="MPLGWFNTTSSGEVRKAVQNDVGELHYLVAHSAVENAGAMATPL
                     FGLIYCFYLDWRLGLLALATIPVYVAVYSWLGRDARTQMERLDRGVERISATIAEFIA
                     GLSVVKTFGEVGKAHKRFADAADEFNDSFANWMGPLVRVKAVTSIFIETPLLLLANLA
                     GGYWFVRSGWVAPIHMLGSTLVAVTIPAAVLTVGYSTHIRKQAQAAAGRLQRLLQTPT
                     LPVVTNPQVPEGNSIEFDGVSFSYDGQRTVLNDVSLTLAPGTITALVGPSG"
BASE COUNT         2666 a         5371 c         5436 g         2850 t
ORIGIN      
        1 cgcgcaaccg tccctacatc ggggcgttgg cgggtacgaa caccccggag ctgtcggcct
       61 cttcctcggc ccggatgacg tgcacgacgg cgttgatcaa cgccaggtgg gtgaacgcct
      121 gcgggaagtt gcccaagtgc cgacctgtgc gcggctcgat ttcctctgca tagaggtgta
      181 gcgggctggc gaacgacagc aaccgctcac acagatgctt ggcccggtgg atctcgccga
      241 tctcgaccag cgccgacacc agccagaacg aacagatcgt gaaggtgcct tcctcgcctg
      301 acagcccgtc gtctgtctcc tcgacgcggt accgcagcac caggccctcc tcggtcagct
      361 cgtcggcgat ggccagcacc gtcgcgcgca cacgcgggtc gtcggcgggc aggaaccggg
      421 tcaggatggc cagcagcagc gaggcgtcca gcgcgtcgtc gccgtaccgc tgcgtcagca
      481 cgccacggga gtcgacaccg ttggccagta cgtcggcctt gatctcctcc gcgatcgccc
      541 gccactgttg cgcgtaggac ttctctccct gcagttcggc cagcttggat ccgcgatcca
      601 gcgccaccca gcacatgatc ttcgacgagg tgaaatgctg cggctcgccg cgcacctccc
      661 agatcccgcg gtcgggctcc ttccagtgct tgatcgcttc ctcgacttgt tgcttgagca
      721 ccggccacaa cgtgtcggag atctgctcgc gcgacttggc atgcagatac acggaatcga
      781 gcatcgtgcc ccagatgtcg tgctgcatct ggttgtaggc gccgttgccg atgcgcaccg
      841 ggcgggcgtt gtcgtagccg gacaggtggt gcagctcctc ttcgacgagg ctgcgttcac
      901 cgccgacggc gtacatcacc tgaagtggat ggcgctcacc gttattggcg cccgacacgt
      961 cggcgatgaa cgcgaagaag tcgtcggcct cgcgatccag gcccagcgtg tacaggcccc
     1021 acagcgcgaa tgtcgagtca cggacccatg cgtagcggta gtcccaattt cgttcgccct
     1081 gaggcgtttc cggcatcgac gtggtcggcg ctgccagcag ggcgccggtg ggcgagtacg
     1141 tcaggccctt cagcgtgagc gcgctgcgct gcagatatga ccgccacggg tggtcgggga
     1201 agtcgccgat gttgatccac tgccgccaac actcgctggt ctgccacatc ttgtcggcgg
     1261 cctcgtcgta ggtctgcggc gccggatgct tggaccagga gagcgcgacg aatgcgttgt
     1321 cgccctcctt cattcgcgtg cgggcgcggg cctcgtggcc ttcgagtccc agccgcaggt
     1381 tggtagtcag gcgcagggtt ggatgcgcgt cggggtcacg gctggcccgc gcgatcgcct
     1441 cgccataggc ctgggccgag tactcccacg tcgcggggat ccggtggtag tcgaacgccg
     1501 gctcgcagtt catcaccaac tcgaccgtgc cgcttacaca tcgcaccgtg cgcaacagga
     1561 tgtgttcggc gtcccaatcc atcggggtac ggcgatgcgt ccgggatcgg gtctcgatgt
     1621 cgtgccacgg gcccatcacc agcgtgtccc gcacgatcat ccagccggtg tgtgtctgcc
     1681 aggtggtctc caagatcagg ctgcccggca ggtaacgacg ggccgcaggc acggacaccc
     1741 cgtacgggct caggcgaaag tggcctgcgc cccggtccag gatggcgccg aagacgctgg
     1801 gggagtcggg gcgggggacg cacagccatt cgaccgagcc ggccgacgag atcaggcagg
     1861 tgttctcgca gtcggacagg aatgcgtagt cagcgatcgg cggaaagggg ttccgcagcg
     1921 ccccgctccc ggtgtacggc accggtgccg tcacggtgaa cggggcctcg gactcgccga
     1981 cgcgggcgga cttcgacccg ttggcctcgg cctccggcgc gccgtcggca gactcggtgt
     2041 cttgcaaggt catgcggaca tcatcgactg ccgagagccc cggcgtccac ttgtcgcagc
     2101 gcgtgtccgg ctgcggttgg ctcagcgccg ggcaaactcc ggcacgtgtt cgggcagcgg
     2161 accgatcgcg accccgtatc ccgccgcggt gcgcagcgcg ggcaccttgt tcgcgatccg
     2221 cacgaaaagc ggcggttcag caccctgacc tgccgaaacg gcgaccgcga tgacacggga
     2281 gtggatgacc cgctgtaccg attggatgag cgcggcgggc aaccatcgcc gcgcctgcac
     2341 gcgggccaac tgcctggtag agacggtgcc gcggcgcagc ggggcggcca gcgcgccggc
     2401 ggccgccacg gcgtcggcga cggccaggtt gatcccgacc ccgccgaccg gtgacatggc
     2461 gtgggcggcg tcaccgatca gcaacagccc atcggtgtac cagcgcctca gccggttgag
     2521 ctgcacatcg agcagcttga cgtcgtccca cgacgtcaca gccttgacgt ggtctgccag
     2581 ccacggcacc agggccacca ccccgcgatg cagcgtttcg atgccctgcg cacgcaactc
     2641 gacgtcacgg cccttcggga tgacgtaggc gcactggaag tagtcgccgc ggtcgatgac
     2701 gatgagtgcg tgcccggcgc cgagcactcc ggccagaccg tgcggatcgt ccgggttgcg
     2761 cggcagccgg aaccaccaga cgtccatcgg cgccccgaag cttctcggcg tcaaccccat
     2821 cgcgtcgcgc agcgtcgacg atcggccgtc gcaggcaacc gtcaacgcgg cgtgcatttc
     2881 gcgcacctgc ccgtcaatgt cgcggtagct gacaccgacc accttgtcgc cgcgccgaat
     2941 cggtgccagc acctcggtgc tgcgcagcag ccgaaacgtc ggttccttgt tgcccgctgc
     3001 ggccagcagc tcaaggagat cccactgcgg aaccagcgca atgtgcttgt gcggacccgg
     3061 aattcggcga agatcgatgg cgacctcggt gccttggatc ttcatgctga tcgagtcgat
     3121 gacgcggtgc ggcatccggg cgaaatcgtc ggccatgccg agttcgtcga gcagccgcag
     3181 ggtgctggca tgcacggtgt cgccgcgaaa gtcgcgcagg aagtcggcgt gcttctccat
     3241 cacggtgacg tccacaccgc cgcgggcgag cagcagaccc agcatgattc cggctgggcc
     3301 gccgccggcg atgacgcagg tggtcctgtc cgcggcgggc acgacgctat ctttccatcg
     3361 aatagtctgg ccgggtgggc ggcttcctca actggtggga cggcgtcgag ctgtggcttt
     3421 ccggtctgcc gttcgtcgcc cagaccgccg tggtgatgcc cgtcgtgctc gccctcgcat
     3481 atggaatcgc catccttctc gatggcgcgc ttggcaatgg gattcggcta ctgcgacggg
     3541 tgcgtcacca aaacgaggac gaggagtgag cggtatgccg cgctctcggg tgaccttggt
     3601 gctcgtcatc ctcgtggtgc tcgtcattgt gatgtggttg ctaactcgtt aagtcggcta
     3661 aggcccaacc tgagcgcggt gccacactct gttattcttc gcgccatgca gacagcgtcc
     3721 ggcaacaaga gcggccacat cctggccctc attgccgtgg gtttcagtgc tgtcgctgat
     3781 gtctgttgtt gtcgctgact ccccatccgt cgctggtttg gtgtcggtag cggctgtctg
     3841 gatgcccagg tactgatccc tcaccttgct gtcacgacgg agttgtcccg ttaatccccg
     3901 caaggaaagg tcaccaatgc tcaacatccg taagtcgtgg cgcgccgcgg ctgtcctcac
     3961 cgccactgcg accgtgctcg cggcgtgcgg tggcggctcc agcgacgtgg cgggcggcgg
     4021 cgatcagccg gccgctgaga cgacgctgac gttggtcgcc tacgcggtgc ccgagcccgg
     4081 ctggagcaag atcatccccg cgttcgccgc cacgcccgag ggcaagggcg tggccgtgac
     4141 cacgtcctac ggtgcatcgg gtgaccagtc gcgcgccgtc gtcgacggca agcccgccga
     4201 catcgtgaac ttctcggtgg agcccgacgt cacccgcctg gtcaaggccg acaaggtggc
     4261 caaggactgg aacgccgacg ccaccaaggg catcccgttc ggctcggtcg tttcgctcgt
     4321 cgttcgcaag ggcaacccga agaacatcaa ggactgggac gacttgctgc agcccgggat
     4381 cgaggtcgtc accccgagcc cgctgagctc cggctccgcc aagtggaacc tgctggcgcc
     4441 gtatgccgcg aagagcaacg gcggcaagga ccagcaggcc ggtttggact tcgtcaacaa
     4501 gctggtcacc gagcacgtca agacgcgccc ggggtccggc cgggaggcca ccgacgtgtt
     4561 cctgcagggc accggcgacg tgctgatcag ctacgagaac gaggccatca acgtcgagcg
     4621 gcagggcaag cccgtcgagc acatcaaccc cccgcagacc ttcaagatcg agaacccggt
     4681 cgcggtcgtc acctccagca ctcacctgga caaggcaacc gcgctgaaga actacctgtt
     4741 caccgccgag ggccagaaga tctgggcgca ggccgggttc cggcccgtcg atccgtcggt
     4801 cgccacggac ttcgccaccg acttcccgac cccgcagaag ctgtggacga tcgctgacct
     4861 gggcggatgg agtgcggtcg atcctgcgtt gttcgacaag gagaacggca gcatcacgaa
     4921 gatctacaag caggcgactg gatgacggct accgtcgaac cgaatccgga ggcgacccgg
     4981 ccagaactca ccgacaacgg tgagggcggc cgggcgcccc gccttctcgg cggacggtac
     5041 gggacgacgt cgctgcgggt cggcgccgca tcgatctggc tgagcgtcat tgtgctgctg
     5101 ccgctggccg cgatcctctg gcagtccgca ggtggcggat gggacgcgtt ctggctggcg
     5161 gtgacatcca acgctgcggt ggagtcgttc cgggtgacgc tgacgatctc ggcgggcgtc
     5221 accctggtga atgtggtgtt cggactgctg gtcgcgtggg tgctcacccg cgacgacttt
     5281 cccggcaagc gcctcgtcga ctcggtgatc gatctgccat tcgcgctgcc gaccatcgtc
     5341 gccagcttgg tgatgctggc cctgtacggc ccgaatagcc cggtcgacct tcacctgcag
     5401 cacaccaagt ggggtgtggg tgtcgcgctg ttgttcgtca cgttgccgtt cgtggtgcgc
     5461 tcggtgcagc cggtgctgtt ggaactcgac cgcgagacag aggaggcggc cgcctccctc
     5521 ggcgccaaca accggacgat cttcacccag gtgatcctgc ccgcgctgct gccttcgctg
     5581 ctgtcgggag cgggcctggc gttctcgcgc gcgatcggcg aattcggctc ggtggtgctg
     5641 atcggtggtg cggtgcccgg cgagaccgaa gtgtcgtcac agtggatccg gacactcatc
     5701 gagaacgacg accgcaccgg ggcggctgcg atttccattg tcctgctggt gatttcgttc
     5761 gtggtgctgt tcattctgcg agcgatcggg tcacgggcgg ccaaacggga ggagctggcg
     5821 ccatgacgct gtcacctgtt gtgcgtcacc tgctgcgcta cctcgcgctg gcctacatcg
     5881 tgatactggt gatcgtgcct gtcgggttga ttctgtggcg cacgttcaca cctggcgtcg
     5941 gcgcatttgt tgcgtcgatc acgacgccgg cggcactctc ggcgctgcaa ctgtcgctgc
     6001 tggtggtcgc gatcgtggtg ccgctcaatg tccttttcgg cgtcccgacg gcactggtct
     6061 tggcgcgcaa caggttccgc ggaaagagtg cgctgcaggc agtgatcgat ctgccgttcg
     6121 cggtgtcgcc cgtggtggtc ggtgtggcgc tgatcctgtt gtggggctcg gcaggattgt
     6181 tcggcttcgt cgagaacaat ctgggcttca agatcatctt cggcttcccc ggcatcgtgt
     6241 tggcgagcat cttcgtcacc gtgccgttcg tcatccgcga ggtcgaaccg gtcctgcatg
     6301 agatcggcac cgaccaggaa gaggcggcct cgacgctggg atcgcaatgg tggcagacgt
     6361 tctggcgcgt caccctcccg tcgatccgct ggggcctgac ctacggcgtc gtgctgacga
     6421 tcgcgcgcac gctcggcgag ttcggcgcgg tcatcatggt gtcgtccaac ctgccgggaa
     6481 cgtcgcaaac tctcacactg ttggtggcgg accggtacag ccgcggcgcg gaatacggcg
     6541 catatgccgt gtccacgctg ttgatgacgg tcgccgtgat cgtcctgatc gttcaggtgg
     6601 tcctcgacgc tcggcgctcg cgagcgagca agcaggtcta aggagacgca gaagaaatga
     6661 ccgacgcagc aatcatcgtg cggggcgcca ataaacgcta cggcgacttc gcggcgctgg
     6721 acaacgtcga cttcgaggtc ccgtccggct cactgaccgc cctcttgggg cccagcggct
     6781 cgggcaagtc cactctgttg cgcgccatcg ccggcctgga cctgccggac accggcacca
     6841 tcacgatcga cgggcacgac gtgacgcaca tcccgccgca gcggcgtggc atcggtttcg
     6901 tgttccagca ctatgcagcg ttcaagcatc tgaccgtgcg cgacaacgtc gccttcggat
     6961 tgaagatccg caagcgaccg aaggccgaga tcaaagagaa ggtcgacaac ctgctggaag
     7021 tggtggggct ggccggtttt cagacccgtt atcccaacca gctttccggt ggccagcgtc
     7081 agcgcatggc attggctcgc gcgctcgcgg tggacccgca ggtactgctg ctcgatgagc
     7141 cgttcggtgc gctcgacgcc aaagtccgcg aggacctgcg ggcgtggctg cgccggctgc
     7201 acgatgaggt gcacgtcacc acggtgctcg tgacccacga tcaggccgaa gccctggacg
     7261 tcgcggatcg cattgcggtg ctcaacaagg gccggattga gcaggtgggc tcgccgaccg
     7321 acgtgtacga cacgccggcc aacgcgttcg tgatgtcgtt cctaggggcg gtgtcgtcgc
     7381 tgaacggctc gctcgtgcgt ccgcacgaca ttcgcgtcgg ccgcaatccc gacatggcga
     7441 ttgcggccag cgacgattcg gtccagacaa ccggcgtggt gcgggcgacg atcgatcgaa
     7501 ttgtcgtgct gggcttcgag gttcgcgtgg aactcaccag tgcggccgac cacacgccct
     7561 tcaccgcgca gattacccgc ggtgacgccg aggcgctcgg gctgaaggaa ggcgacacgg
     7621 tgtacgtgcg tgccacccgg gtgccggcat tgccgggcgg aaccgaactc ccgcgggcgg
     7681 acaaggccga cgagggagag ctgacgacgg cctgacgctg tcagctgtcg acggaaccgg
     7741 gcatctggag aacgagcttg tgtccgggag gctgcgtgcc gatatcagta tgtgcgccaa
     7801 cgacctccgt gaatggcact gtggtcacgg cgggaccacg aagagcgcca tcggcgatcc
     7861 ggttcagttg gtcggtgagg atctgccgat acttctccgg ctgcagtgcg acaagcaagg
     7921 gcactgaaca cactcgttca cgcggcgagg cataccgacg caggttggcg aacagaccgc
     7981 cgacggccgc gtcccgccgt ccgtgcccgg gccgcccgga ccacgccgtc cgtaccaggg
     8041 tgccgtgtgg cgctatcgcc gcccgtgccg cggggccgcc ggtgtggtcg atgccggcgt
     8101 cggcaccgcc cgacgtcagc tcccgcacct gcacgggcca gttcggatcg tggtaatcca
     8161 cgacatgcgc accgaaagac tcggcgaaac cccgtgtgcg ttccgatgcg gtgccaacga
     8221 ctttcacgtc attggcgcta ccgtattggc tgaccagggc accaaccgaa cccgagacgc
     8281 cctgcacgaa aatggtccca ccgcttggtg cccggcccag ttcgatgcac agtgccgcgg
     8341 tcaccagatc aagcggcaac gcggctacct gagccgagtc gagtgaatcc ggcaccggta
     8401 caagacgatt cgacggcaga acgagttccg ttatgtagct gcccatccgg gccaggcacc
     8461 cggtgacgcg catgcccggc cgcagtccgc ggcggtccgc tgcggtatca accgtttcca
     8521 gtacgccgac gaagtcatag ccgggaacga aacccggccg cggttggagg agatagtcgc
     8581 ccgagcgcgc cattgcgtcg gtggacccga ccgaggcatg cgtcacccga actcgcacct
     8641 tcttgcgact cggccgcgca ggcgtgacct cgtcgatttc caggacgtct ggccctccga
     8701 agcgactgac ccgcgcgcgc aatactttgg tagtcacgcg acttacggta cagtctgcat
     8761 cgtatgtctg gcaagacggg ccgcccgcgc gacacctctc tcgacgccgc catcgctgcg
     8821 gcgacggagt cggtgctgct agccgagggt tacgcggccg tgtccattga ccgggttgcc
     8881 aaaatcgccg gtacgacgcg tgccgctgtc taccgccgcg tcaagagccg cgcggaactt
     8941 gtcgtcggcc tgctgatcca tcgcttcggc acgtcgcccg cgccggacac cggcgacttg
     9001 cgccaagaca tcgaacacgt ccaagcggtt caacgcatct tcttcgccga ggccatcgtg
     9061 cgtgccgggc tcgccggtct gctcagcgac acctatgccg accccaccct cggcgcggct
     9121 ctgtacgacc gtttcctcgc gcccaggcgg cactcggtgg cagcgatgct cgcccgagcc
     9181 gccgaccgcg ggcagatcac tcccgtagac gaccccgccc gcatctccga catcctcacc
     9241 ggaccgctgc tgtatcgcac tctggtaccg gccatcgggc aaatcgatga cgcgctggtc
     9301 gaagccaccg ttaatgccgc ccttagtgcg gtgtcttctt aggcggcgac gcgttcggct
     9361 agtgcctgat cgaagcgatc caacaccgta tccgccacca agcgatgcgg gccaagcggc
     9421 gctgccatcg gaatccgttg agcgcgtgcg aagttcgtga ctcggtcggt gatccggccg
     9481 tgcgcgagga accagggggc gatgaccaat cgggtggcgc cgcgctcgcg cagaatgtct
     9541 gtggcctcgg ccagcgtcgg gtgcggcccg gtggcgaagg cggtggtggc ggtccacctt
     9601 gtgtgttgga cgagatagtc ggcgaccaaa ctggtgcgcg cattggcttg cgggcgtgag
     9661 gatcccaccg ctgtgacgag gacgcccacg tcggggtcga gttgcgatac cccggcgtgt
     9721 gtcaggcgtt gccgaagcac tgctacgagt cggtcgtcct cgccgagcac gtcggcctga
     9781 atggcgttcg ccccggactc tgcgatgagc gccgggatgt ccacgcgagc gtgatacgcg
     9841 tcggcgagca gcagtggcac cacaaccgcg ttgctccctg tcgctgccag cacgtcccgc
     9901 agatcgggga agttctgctc gcagaatgcc acccgaacgt cgacatcacg ccgaatcatg
     9961 cggatggtgt gcgccacctc gtgggccgat gccgcggatc gcgggtcggc actaccgtgc
    10021 gccgtcagga caagcgatgt cacgaagcgt gtaacccgca ttccgtcttc gatgtgccgg
    10081 cccatcggcc gctgcgcggg tcggcgcctt caacaggctt cgccgtgcac ggcgcgcagc
    10141 cgattgacgg atagccttcg aacacaaggg gattgaccag cacatcgttg gcatcgatgt
    10201 agttctgcat gtcctcgtcg gtccatgcgg ccaacggatt gatcttcacc aacccgaatg
    10261 ccttgtccca gctgatgagt ggcgcattgg cgcgcgtcgg cgcctcgacg cggcggatgc
    10321 ccgtcaccca cgccgagtag ccccgaagcg ccttgctcag cggctcgacc ttacgcatcc
    10381 ggcagcactc gttgggctcg cgggcgaaca ggtccttgcc gaacaacccg ttctgctttt
    10441 cgaccgagtt ctccggcgtg acgttgacga cgttgatgtc gtagaccgcc tcgaccgcgt
    10501 cacgggtgcc gatggtctcg acgaagtggt agccggtgtc caggaacagc acatccacac
    10561 cggggcggac cttggcggcg agatcgacga gcaccgcgtc ctgcatattg gaggccacga
    10621 tgtaattgcc gccgaagttc tcatctgtcc agcgcagcag gtcgagggcg ctcgcaccct
    10681 ccaactccgc ggccccgcct tccgccagtg cttgtagatc gctgtccgaa atcaaatctg
    10741 tcatcgcaaa tccgcctcgt ctgctcgtac tgcccacgta gcgaaacgct cgccctgctc
    10801 acgttgtttc acgaagttcc gaacaacccg gtcgatgtag tcgccgagct ccgtcgacag
    10861 caccttgtgc tgacgcagct tgcggccgaa cccgctgtcc aggcccaggc tgccgcccag
    10921 atgcacctgg aaaccctcga ccgacccacc gtggccgtcg tcgaccatct gccccttgaa
    10981 cccgatgtcg gcgacctgaa tgcgggcgca cgagttgggg cagccgttga tgttgaccgt
    11041 gatcggcacg tcgagctggg cgttgatgtc ctccagccgc ttctccagct cgggcacgag
    11101 cacctgggcc cggttgcggg tctcggcgaa cgacagcttg cagtactcga tgccggtgca
    11161 ggccatcaga ttcttacgcc acgccgacgg ctgggacggc aggccgagcg cgtccaggcc
    11221 ggcgaccaac tcgtcgagct tctcgtcggc gacgtcgagg atgaccagct tctggtacgg
    11281 ggtgaaccgg acccggtccg aacccgcggc ctcggcgagg tcggccacct tcgtcaggat
    11341 ggtgcccgtg acccgacccg cgatgggcgc gacgccgacc gcgttcttcc cgttcttcaa
    11401 cttctgaacg ccgacgtggt cgatcgggtg cttgacgggc tccggcgccg gaccgtcgat
    11461 cagtttgcga cccaggtatt cgtcctcgag tacttggcgg aacttctcga taccccagtc
    11521 cttgatcagg aacttcagcc gcgccttggc ccgcagccgc cggtagccgt agtcgcggaa
    11581 gaccgacgtc accgcctccc agacgtcggg cacctcctcc agcggcaccc acgcgccgac
    11641 ccgctgggcc agcatcgggt tcgtcgacaa gccgccgccc acccacaggt caaggccggg
    11701 accgtgctcg ggatggttga cgccaatgaa tgcgacgtcg ttcacctcgt gcgcgacgtc
    11761 ctgaaggccc gagatcgcgg tcttgaactt gcgcggcagg ttggagagct cgggactgcc
    11821 gatgtaccgg cggacgatct cgtcgatggc ccatgtcggg tcgagcactt cgtcgaccga
    11881 ctcaccagcc agcggcgaac ccagcaccac gcgggggcag tcgccgcacg cctccgtggt
    11941 ctgaaggccg accgcggcca gccgctgcca gatctccggc acgtcctcga tctggatcca
    12001 gtggtactgg acgttctctc ggtcgctgat gtcggcggtg tcgcgtgcga actcggtgga
    12061 gatctcaccg agcgtgcgca aggcctgtgt ggtcagcgcg ccgccatcgc agcgcacccg
    12121 catcatgaag tatctcgcct cgaggacgtc ggcgttctcg tcgccggtcc acgtaccgtc
    12181 gtaaccctgt tcacgctggg tgtagagacc catccagcgc atccggccgc gcagatcctg
    12241 cttgtcgatg ctgtcgaagc cccgcttgga gtagatgtca agaatgcggg cccgcacgtt
    12301 cagcgcgtcg tcatccttct tgaactgctc gttggggttg agtggctcgc ggtaaccgag
    12361 cttccactgg ccctcgccac gcgggcgctt gacgggcttt gcggtggtca tatggtgtgg
    12421 ctcctctgtg gagccggatc gcgcgttctc accggtgggc tgtccggcgg cgcagatccg
    12481 gcggccagct gaaagtacag gtgggcaggt ctacgacgtc aaggcagaca acaacaggta
    12541 caaacgcgct tgaagtcgac gtgccgccga gccaccaacg agacgcggtg ggggaaacgg
    12601 gcggcgatca cgtcgccaat tgtgccacgg acaaccgtca cggccctaac cgggcgagat
    12661 ttgcgctcac ggtgagcaaa agtcggctct gggagactgg ctacatgacg gtccgaacgg
    12721 ctccgctgca gctgcccgac ctggccactg cgccgggtcg gccgttcggc atctatatcc
    12781 acgtgccctt ctgcgcgacg cgctgcggct actgcgactt caacacctac acgcccgccg
    12841 agctgggcgg cgcgaacccg gacggatggc tggaggcgct gcgggtcgaa ctcgccctgg
    12901 cggcatcgcg gctgggctca ccacctgagg tcgacacggt gttcgtcggc ggcgggacgc
    12961 cctcgctgct gggtggggac ggcctgcggg cggtgctcga cgcgatccgg gcgcatttca
    13021 cgctggccgc ggacgtcgag gtgacgacgg aggcgaatcc tgagtccacg tcgccgaggt
    13081 tcttcgaaca gctgcggtcg gcggggttca cccgggtgtc gctcggcatg cagtccgtgg
    13141 cctcgcacgt gttggcggtg ctggatcggg tgcactcgcc gggccgggcg ctatccgcag
    13201 ctgttgaggc ccgcggcgcc ggcttcgagc acatcaacct ggacctcatc tatggcacgc
    13261 cgggggagtc cgacgacgac ctgcgccgct ccgtggacgc ggcgatcgac gcaggcgttg
    13321 atcatgtgtc tggatacgcg ttggtggtcg aagacggcac ggcgctggcg cgccgggtgc
    13381 gtcgcggcga aatcgccgcc ccggacaacg acgtcctggc gcagcgctac gagttgctcg
    13441 atgaacggct gtcggcagcg ggcttcgact ggtatgaggt ctccaactgg agcaggcccg
    13501 gtggcgaatg ccgacacaac ctcgggtact ggaacggcgg cgaatggtgg ggcgccggac
    13561 cgggagcgca tggttacgtc ggcaccacgc ggtggtggaa tgtcaagcac cccaacgctt
    13621 atgcgcaatc gctggccgac gggctgctgc cggtggccga tttcgagcaa ctggacgcca
    13681 cggcgttgca cgttgaggat gtgatgctgc ggctgcggct gcgcgacggc ctgccggtgg
    13741 ccgtgttggg cgacgaggaa cgggcccgag cggagacggt gatcggcgac ggactggtcg
    13801 aattgcacgc cgaccgactg gtgctgacgg accgcggccg gctgttggcc gacgccgcgg
    13861 ttcgcacgct gctcgactag ccgccgagtg tgggcttggt gcacgcttct gcgaattttc
    13921 gcgtgacata agtccacact cggcgtcagg ctgattgaac gacctgggct gtcggcccgt
    13981 tgtgctcatc gaaggccgac cgaaagcaag ggatgtgcgg tgcgtgaagc atgactgacg
    14041 attccgagca ggcggcgctg ctgcgggccg tgcacgacga gcacggccag tcgctgctgc
    14101 gttacgtgtt gagactgacc aggggtgata tgccgttcgc cgaagatgtg gtgcaggagt
    14161 cgctgttgcg gctctggcgt aagccggagg tgctgcagca gccgacggac aacgtgcgcg
    14221 cctggttgtt caccgttgcg cgcaatctgg tgatcgatga ccggcgcagc gctcgctaca
    14281 cccgcgagtt gcagagcgag tacctccccg agcggccgtc gcccgacgcg atcggcccgg
    14341 cgttcgacaa atggatactg tcggacgctt tgacgtcgct gtcgccggaa catcgcaccg
    14401 ctattgtgcg cgcctactat ctcggccaaa cagtcgcgga cattgcgcag caagagggaa
    14461 ttccggaggg aacggttaag tccaggcttc attacgcgct tcgcgcgttg cgaattgcgt
    14521 tgcaagaaag gggggttgtg cggtgaccag ctatccaccc gaagacaacc ttgccgaatg
    14581 ggacgccgcc tatgttctgg gcgcgctgag cctggaggag cggcggacgt acgaaaatta
    14641 cctcgctgcc aacccggcgc gggctgcgga gctcaccgag ctcgccggga tgcccggcat
    14701 tctcaacgcg ctgagccgtg acgaagccgt cgcgctgacc gaccttgccg gcgcgccgcc
    14761 cgttgaaagt cggccggaca acgttgcctc gcttgcccaa gctgcggcca agcggcagca
    14821 gcgatcgcgg cgcacgtggc tagcggcggc ggtggggtcg gcagccgcgc tgttgattgc
    14881 cggcggcatc gtggggtcga cggtcttccc gcagtcctcg acaccgacgc agacggtggc
    14941 gatgcaggcc atgcagccga ccccgcgcgg cggcctgacc gccgaactcg cggtgaccga
    15001 gaagaagtgg ggcacggagc tgaactgggc ctgccagtac accaaggact ggtcacgcaa
    15061 cgtcgacagc tacgacatcg tggtgacgac gcacgacggc gtgcagcaga cggtgggctc
    15121 gtggaagccg gccggagatg aggcgacggg actgtcggcg gcgacgagca tcccgacgaa
    15181 gcagattcgc acggtcgaca tccgagtgac cggcagcaac gagccgttgg cgatcaagac
    15241 gttgcggtag ctcccaattt gcggccgcca cccactagcg ttaggtaaac ctaacctaca
    15301 ctgctggagg gtggatgacg gctgtgcacg cggcgctcga ggtcgaaccc gatcaatcgg
    15361 ggcagccagc caacagccgc gccacattcg atgtgctggc gctgcagcag ctgctggctc
    15421 cggttcggcg ggtcaccgcc aacgctcaac ggatccagct gatcgcgtcg gccgccacgg
    15481 tgtgccgttc gtcggcatcg tcgataccct cggcccgttg ccgcttggct ggttcaacac
    15541 cacgtcatcc ggagaagtcc gcaaggccgt ccagaacgac gtcggcgaat tgcattacct
    15601 ggtggcgcat tcggcggtcg aaaatgcagg cgcgatggcg acgccgctgt tcgggctgat
    15661 ctactgcttc tacctcgact ggcgccttgg actgctcgcg ctcgcgacca ttccggtcta
    15721 tgtcgccgtg tactcatggc tgggccgcga cgccaggacg cagatggagc ggttggaccg
    15781 cggcgtcgaa cggatcagcg ccaccatcgc cgaattcatc gcaggcctgt ctgtggtcaa
    15841 gacgtttggc gaagtgggca aggcgcacaa gcgatttgcc gacgcggccg atgagttcaa
    15901 cgacagcttc gccaattgga tgggaccgct ggtcagggtc aaagccgtca cgtcgatctt
    15961 catcgaaacg ccactgctgt tgctggccaa cctggccggc ggttactggt tcgtccgcag
    16021 cggctgggtg gcgcccatcc acatgctggg ttcgacgctg gtcgccgtga ccatccccgc
    16081 cgcggtgctg accgtcggat actccaccca catccgcaaa caggctcaag ccgcggcggg
    16141 ccggctacag cgtctgttac aaacgccgac cctgcccgtc gtcacgaatc cgcaagttcc
    16201 cgaaggaaac tcgatcgagt tcgacggggt cagcttctcc tacgacgggc agcggacggt
    16261 cctcaacgac gtctcgctca ccctcgcccc gggaacgatc accgcactgg tggggccgtc
    16321 ggg
//
LOCUS       DASRJA010000161         4730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1619077,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000161 DASRJA010000000
VERSION     DASRJA010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619077"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..983
                     /locus_tag="VFC01_07645"
     CDS             78..983
                     /locus_tag="VFC01_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HZN79545.1"
                     /translation="MPEFEFVTLRDPSSSLTATYVPNAGMVGTSLSDDSVELLGQRRG
                     LQAYVSTHRTMGIPILYPWANRLSSNGYGVGGAVVTLTPGTGGVRTDQHGVPIHGTLA
                     GYPDWNVTTQLESRLTAELDYAARPGLLATFPFPHLLELDITLADRTLTVKTTVTATT
                     GSRVPLCFGFHPYLQLPDVPRAQWQMETPAMRFRPVNAWGIPTGEMEPHEATSELLGD
                     KFFDDGYDDVKPGSIFAVSGGNRRIEVHFDEGYPAAQVFAPGDDDVVCFEPMAAPTDS
                     LRRGGYRVVNPSESAVAQFRIKVSS"
     gene            980..1882
                     /locus_tag="VFC01_07650"
     CDS             980..1882
                     /locus_tag="VFC01_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HZN79546.1"
                     /translation="MTDLTPVTLHDPSSPLSVVYVPGAGMIATSLSDGGTELLGQRRG
                     LDAYVSAAKTMGIPLLYPWANRLSARTYDVDGQTVTLAPDAYGVHADNNGLPIHGVLA
                     AYPGWRAQQVSDQKITAELDFGAHEELLASFPFPHRIELTVELADRTLTVSTTVTATT
                     DRAVPLVYGYHPYLQLSDVPRADWQVEMPTMRHLVLDGKGIPTGATEEWPASAEPLGD
                     NVFDDGFDQVADGAVFAVSGGGHRLEVRFERGYPTAQVFAPAGETVICFEPMTAPTNA
                     LHAGGYRSARPGEPDTTLFSITVS"
     gene            complement(1875..2258)
                     /locus_tag="VFC01_07655"
     CDS             complement(1875..2258)
                     /locus_tag="VFC01_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79547.1"
                     /translation="MAKKDDARTFLISVAAELAGMHAQTLRTYDRLGLVSPQRSSGGG
                     RRYSQHDVDLLREVQRLSQDEGVNLAGIKRIIELTNQVEALQSRLRELAEELDRLRAG
                     QRRDVAVMPKSTAVVVWQPRNKRSS"
     gene            complement(2258..3427)
                     /gene="dnaJ"
                     /locus_tag="VFC01_07660"
     CDS             complement(2258..3427)
                     /gene="dnaJ"
                     /locus_tag="VFC01_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZN79548.1"
                     /translation="MAQREWVEKDFYKELGVSSDASADEIKRAYRKLARDLHPDTNSD
                     PSAAEKFKAVSEAHSVLSDPAKRKEYDETRRLFAGGGFGRGRFSGGNFGGFGGDGAEF
                     NLNDLFDAAGQTGGANIGDLFGGLFGRGAQQARPSRPRRGNDLETETELSFMEATKGV
                     AMPLRLTSPAPCTNCHGSGARPGTSPKVCPNCNGSGVVSRNQGAFGFSEPCTECRGSG
                     SIIEHPCDECKGTGVTTRTRTINVRIPPGVEDGQRIRLAGQGEAGLRGAPSGDLYVTV
                     HVRPDKVFGRDGDDLTVTVPVAFHELALGTTLSVPTLEGKVGVRVPKGTSDGRILRVR
                     GRGVPKRSGGHGDLLVTVKVAVPQNLEGEAAEALESYAKAERASGFDPRAGWAGA"
     gene            complement(3468..4109)
                     /gene="grpE"
                     /locus_tag="VFC01_07665"
     CDS             complement(3468..4109)
                     /gene="grpE"
                     /locus_tag="VFC01_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZN79549.1"
                     /translation="MSQHDSHEPVTVTDKRRIDPETGEIRESATGPAPSGPAPAGAAA
                     GAAQRADASASEEVTELKSTLQRVKAEYDNYRKRSLRDQQLAAERTKAAVVSQLLPVL
                     DDLDRARSHGDLESGPLKSVADKLATALEGLGLSGFGEEGDAFDPALHEAVQHEGEGT
                     HPIVGTVMRRGYRVGDQVVRHALVGVVDTVPDAEEVADTLDNGDEPADEAAES"
     gene            complement(4106..>4730)
                     /locus_tag="VFC01_07670"
     CDS             complement(4106..>4730)
                     /locus_tag="VFC01_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZN79550.1"
                     /translation="IQVYQGEREIASHNKLLGSFELTGIPPAPRGVPQIEVTFDIDAN
                     GIVHVTAKDKGTGKENTIRIQEGSGLSKEEIDRMIKDAEAHADEDRKRREEADVRNQA
                     ESLVYQTEKFVKDQREAEGGSKVPEDTLSKVDTAIAEAKTALEGTDISAIKSAMEKLG
                     VESQALGQAIYDATQAEQAAGGGADAGGPSQDDVVDAEVVEDDRENK"
BASE COUNT          732 a         1629 c         1491 g          878 t
ORIGIN      
        1 ctgatcgcca aaggcgcacc agccgaacgc atccaacacg atccgctagc ccgttgagcg
       61 tggcaggatc gacaccaatg cccgagttcg agttcgttac tctgcgtgac ccctcgtcgt
      121 cgttgaccgc gacgtacgtg ccgaacgccg gcatggtcgg cacgtccctg tctgatgaca
      181 gcgtggaact cctcggccag cgtcgtggtc tgcaggcgta cgtatcgacc caccgcacga
      241 tgggcattcc gatcctttat ccgtgggcga atcggttgag cagcaacggg tatggcgtcg
      301 gcggcgccgt cgtcacgcta acgccgggca ccggcggtgt gcgcaccgac cagcacggcg
      361 tgccgatcca cggcacgttg gccggctacc ccgactggaa cgtcaccacg cagctcgaat
      421 cccggttgac cgccgaactg gattacgccg cgcggccggg gctgctggcc acgtttccgt
      481 ttccacacct gttggagctc gacatcacgc ttgcggaccg cacacttacc gtgaagacga
      541 cggtgacggc aacgaccggc tcgcgcgttc cgttgtgctt cggctttcat ccgtatctac
      601 agctgcccga tgtgccgcga gcgcagtggc agatggaaac gcctgcgatg cggttccgac
      661 ccgtcaacgc ctggggcatt ccgaccggcg agatggaacc gcatgaggcc acctcagagt
      721 tgttgggaga caagttcttc gacgacgggt acgacgacgt gaagccgggt tcgatcttcg
      781 cggtgtcggg cggaaaccgt cgcatcgagg tgcacttcga cgagggctac ccggccgcac
      841 aggtattcgc gcccggcgat gacgacgtgg tctgcttcga gccgatggcc gccccgaccg
      901 actcgctgcg ccgcggcggc taccgagttg tcaatcccag tgagtccgcc gttgcacagt
      961 tccggatcaa ggtgagctcg tgaccgatct gacgcccgtc accctgcatg acccgtcatc
     1021 tccgctgtct gtggtgtacg tgcccggtgc aggcatgatc gcgacgtcgc tgtccgacgg
     1081 cggtaccgaa ctgctcggcc agcgccgtgg cctggacgcc tacgtatcag ctgcaaagac
     1141 gatgggcatc ccgctgctgt atccgtgggc aaaccgattg agcgccagga catatgacgt
     1201 cgacggccaa accgtcacgc ttgcccccga cgcctatggt gtgcacgctg acaacaacgg
     1261 actgcccatc cacggtgtgc tggccgccta ccccggatgg cgagcacaac aggtctccga
     1321 ccagaagatc accgctgaac tggatttcgg cgcgcacgag gagctgctcg cgagcttccc
     1381 gttcccgcat cggatcgagc tgaccgtcga actagccgac cggacgctga ccgtcagcac
     1441 cacagtgaca gcgacgaccg atagagcagt accactggtg tacgggtacc acccatatct
     1501 acagctgtcc gacgtgccgc gggccgactg gcaggtcgag atgccaacga tgcgtcacct
     1561 cgtcctcgac ggcaagggca ttccaacggg cgcgacggaa gaatggccgg ctagtgcgga
     1621 gccgctgggc gacaacgtgt tcgacgatgg gttcgatcag gtcgccgacg gcgcagtatt
     1681 cgcggtttcc gggggcggac accggctgga ggtgcgattc gagcggggtt atccaaccgc
     1741 gcaggttttc gcgcccgcgg gcgaaaccgt gatttgcttc gaaccgatga ccgcgcccac
     1801 taacgcactg cacgccggcg gctaccgatc cgcccggccg ggtgagccgg acacgacgct
     1861 gttttccatc accgtcagct agagcgcttg ttgcgcggtt gccagacgac cacggccgtg
     1921 ctcttcggca tcaccgcgac atcgcggcgc tgcccggcgc gcagccggtc gagctcctcg
     1981 gcgagttcac gcaggcgcga ctgaagcgcc tcaacctgat tcgtcagctc gatgatccgc
     2041 ttgatgccgg cgagattgac accctcgtcc tgcgacagcc gctgcacttc ccgcagcaga
     2101 tcgacatcgt gctgcgagta gcggcgcccg ccgccggagc tgcgctgcgg gctgaccagg
     2161 ccgagccggt cgtaggtacg cagcgtctgt gcgtgcatac cggccagctc ggcggccacc
     2221 gagatcagaa acgttcgggc atcgtccttc ttggccatca tgcgcccgcc catcccgcac
     2281 gcgggtcaaa cccgctggcg cgttccgctt tcgcgtagga ctccaacgct tcggccgctt
     2341 cgccctccag gttctgcggg accgctacct tgactgtgac gagcagatcg ccatggccac
     2401 cagagcgttt cgggacccca cggccccgta cccgcaggat gcggccatcg gacgtgcctt
     2461 tgggcacccg cacgccgacc ttgccttcca gggtcggcac cgaaagcgtg gtacccaagg
     2521 ccaactcgtg gaaggcgaca gggaccgtga ctgtcaggtc gtcgccgtcg cggccaaaca
     2581 ccttgtccgg tctgacatgt acggtcacgt acaggtcgcc ggatggggct ccgcgcaatc
     2641 cggcctcgcc ctgccctgcc aaccggatcc gctggccgtc ctcgacaccc ggcgggatcc
     2701 gcacgttgat ggtgcgggtg cgagtggtca ccccggtgcc cttacattcg tcgcacggat
     2761 gctcgatgat cgagccgctg ccccggcatt ctgtgcaggg ttcggagaac ccgaacgcgc
     2821 cctgattgcg gctgacgaca ccggacccgt tgcagttcgg gcacaccttc gggctggtgc
     2881 ccggacgcgc cccgctgcca tggcaattcg tgcatggcgc cgggctggtg agccgcaacg
     2941 gcatcgccac acccttggtg gcctccatga acgacagctc ggtttcggtc tccaagtcgt
     3001 tgccgcgccg cggccggctg ggccgtgcct gttgtgcgcc acgaccgaac agaccaccga
     3061 acaggtcgcc gatgttcgcc ccgccggtct ggccggccgc gtcgaacaag tcgttcaggt
     3121 tgaactcggc gccgtcgcca ccgaatccgc cgaagttgcc tccactgaag cggccccggc
     3181 cgaatccgcc gcccgcgaac agccgtcggg tttcgtcgta ttccttgcgc ttagccggat
     3241 ccgacagcac gctgtgcgcc tcggacaccg ccttgaattt ctcggcggcg ctcgggtcgg
     3301 agttggtgtc gggatgaaga tcgcgggcca atttccggta ggcccgcttg atctcgtcgg
     3361 cgctggcgtc agaggagacg ccgagctcct tgtagaagtc cttttcgacc cattcgcgtt
     3421 gggccattac cgcgtcacct ccttacctct tctcttagca gtagttctta cgattctgcg
     3481 gcctcatccg ccggctcgtc gccgttatcg agcgtgtccg cgacttcctc ggcgtccggc
     3541 acggtgtcga ccacgccgac gagggcatgg cgcaccactt gatcgccgac cctgtatccg
     3601 cgccgcatca cggtgccgac gatcggatgc gttccctcgc cctcgtgctg aacggcctcg
     3661 tgcagcgccg ggtcgaaggc gtcgccttct tcgccgaatc ccgaaaggcc aagcccctca
     3721 agggcggtcg ccagcttgtc agcgaccgac ttcaaggggc cggactccag atcaccgtgg
     3781 cttcgggcgc ggtcaagatc atcgagcacc ggaagcagtt ggctgacaac agcggccttg
     3841 gtccgctcgg cggcgagctg ctggtcgcgc agcgagcgct tgcggtagtt gtcgtactcc
     3901 gccttgacgc gctgcagggt ggatttcagc tcggtgactt cctccgacgc cgacgcatct
     3961 gccctttgcg cggctccagc cgcggccccc gccggcgccg gcccactggg ggccgggccg
     4021 gttgcggatt cacggatttc gcccgtctcg ggatcgatac gccgtttgtc ggtgacggtc
     4081 accggctcgt gcgaatcgtg ttggctcact tgttctcccg gtcatcttcg acgacctcgg
     4141 cgtcgacgac gtcgtcttgc gagggcccac cggcatctgc gccgccgccc gcagcctgct
     4201 cggcctgcgt ggcgtcgtag atcgcctggc cgagagcctg cgactcaacg cccagcttct
     4261 ccatcgcgga cttgatcgcg gagatgtcgg tgccttccag cgccgtcttg gcctcggcga
     4321 tcgcggtgtc gaccttggac agggtgtcct cggggacctt cgatccgccc tccgcctcac
     4381 gctgatcctt gacgaacttc tccgtctggt acaccagcga ctcggcctgg ttgcggacgt
     4441 ccgcttcctc gcgacgcttg cgatcctcgt cggcgtgcgc ctcggcgtcc ttgatcatcc
     4501 ggtcgatctc ctccttggac aggccggagc cttcctggat gcggatcgtg ttctccttac
     4561 cggtgccctt gtccttggcg gtgacgtgga cgatgccgtt ggcgtcgatg tcgaaggtga
     4621 cctcgatctg cggcacgcca cgcggggccg gcgggatgcc ggtcagctcg aagctgccga
     4681 gcagcttgtt gtgcgaggcg atttcgcgct caccctgata gacctggatc
//
LOCUS       DASRJA010000162        20127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1648495,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000162 DASRJA010000000
VERSION     DASRJA010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20127
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648495"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1795)
                     /locus_tag="VFC01_07675"
     CDS             complement(<1..1795)
                     /locus_tag="VFC01_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactan 5-O-arabinofuranosyltransferase"
                     /protein_id="HZN79551.1"
                     /translation="MRGALAVIGQMAVAVAVAVVVAVVSLVAISRVEWPAYNSSNQLH
                     ALTTVGQFGCLAGILATGWIWRRGRRLLAKAAAVVFLSAFAVVTLAMPLGATKLYLYG
                     ISVDQQFRTEYLTRLTDTAGPHDMTYYGLPPFYPPGWFWLGGRLAALTGMPAWEVFKP
                     WAIISITIAVVLAFVLWASMVRFEYALIVSTATAAAALAYSPTEPYAAIIAVLLPAVF
                     VLAWSGLRGGTRNGGWAAVIGVGIFLGIAALFYTLLFGYAAFTLAVMGVLLAVARRRL
                     EPLLRLVVIAVISGAIALLGWGPYLWAALHGQPADSGTAQHYLPEAGASLEFPMLRFT
                     LLGALCMLGTLWLVWRARSSTRAGALTVAVLAVYAWSLLSMLRTLVGTTLLSFRLEPT
                     LTILLTAAGAFGFIEVTRALAARVRPENARRVVAAAATVGAIGALTFSQDIPDVLRSD
                     IVVAYTDTDGYGQRADRRPPGAEQYYREIDTKIRETTGLPRDETVLLTADYSFLSYYP
                     YYGFQGLTSHYANPLAQFDQRAAAIESWATLTTADQFIAALDKLPWKAPTVFLMRRGA
                     SDTYTLRLASDVYPNQPNVRRYHVELDNALFD"
     gene            complement(1798..2580)
                     /locus_tag="VFC01_07680"
     CDS             complement(1798..2580)
                     /locus_tag="VFC01_07680"
                     /EC_number="1.1.1.333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenylphospho-beta-D-erythro-pentofuranosid-
                     2-ulose 2-reductase"
                     /protein_id="HZN79552.1"
                     /translation="MVLDAVGNPQTILLLGGTSEIGLAICERYLRNASARIVLAELPD
                     HPRRDTAVEQMKAAGAKSVEVIDFDAVDTDSHPKVIDAAFAGGDVDVAIVAFGLLGNA
                     EELWQNQRKAVQIAEINYTAAVSVGVLLGEKMKAQGFGQIIAMSSAAGERVRRSNFVY
                     GSTKAGLDGFYLGLGEALREYGVRVLVIRPGQVRTTTTIEHWKSTGAKEAPFTVDKED
                     VAELAVTASAKGKELVWAPGAFRYVMMVLRHIPRPIFRKLPI"
     gene            complement(2581..3945)
                     /locus_tag="VFC01_07685"
     CDS             complement(2581..3945)
                     /locus_tag="VFC01_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN79553.1"
                     /translation="MFETSTQRLTGWARTAPSVAQVLSTPDPEVIAKAVSQADSRGVI
                     ARGLGRSYGDNAQNGGGLVIDMNALNKIHSIDADSHLVDVDAGVNLDQLMRAALPCGL
                     WVPVLPGTRQVTIGGAIACDIHGKNHHSAGSFGNHVRSMDLLTADGEIRQLTPDGPES
                     ELFWATVGGNGLTGIILRAIIEMTPTETAYFIADGDVTASLDETIAFHSDGSEDNYTY
                     SSAWFDAISAPPKLGRAAISRGSLATIDQLPKKLQRNPLKFDAPQLLTIPDIVPNGML
                     VGKYTFMPIGELWYRKSGTYRGKIQNLTQFYHPLDMFGEWNRAYGPAGFGQYQFVVPT
                     EAVEPFKGIMYDIQQSGHYSFLNVFKLFGPGNQAPLSFPIPGWNVCVDFPIKPGLNEF
                     LNELDRRVLEFGGRLYTAKDNRTTAETFHAMYPRIDEWIAVRRKVDPNGVFASDMARR
                     LELL"
     gene            complement(3977..4375)
                     /locus_tag="VFC01_07690"
     CDS             complement(3977..4375)
                     /locus_tag="VFC01_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZN79554.1"
                     /translation="MSPNLTLKTQAWRFIVTGGFAAIVDFGLYVLLYKAVGVQVDLAK
                     AISFIFGTITAYLINLRWTFQAEHGIARFIAVMALYGITFAVQVGLNHYCLHLFDYKA
                     WAVPVAFVIAQGTATVINFIVQRAVIFRLR"
     gene            complement(4400..5806)
                     /locus_tag="VFC01_07695"
     CDS             complement(4400..5806)
                     /locus_tag="VFC01_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZN79555.1"
                     /translation="MDSWANSGTRDLHLDLRQTITPGARGARDLLLSALRDAVRSGRL
                     APGTMLPPSRTLATDLGLARNTVAEAYAELVAEGWLASRRGAGTWVVNVSGADIPATP
                     RGVRVVPTHNLSPGSPDVSEFPRTAWVAATRRALADAPTEALRMGDPRGRPELRNALA
                     EYLARARGVRTSPESIVICAGVRHAVELLTRAVGGPIAVEAYGLFIFRDAIDALGTST
                     VPIGLDEQGAVISDLDELDVPAVLLTPAHHNPQGMPLHPSRRTAVIDWAQRTGGYVFD
                     DDYDGEFRYDRQPVGALQALCPDRVAYLGSTSKSLAQTLRLGWTALPDELIDPVIAAA
                     GGAQFYVDAINQLTMAEFIASGQYDRHIRRMRGRYRRRRDTLIDALAGFDVGISGLAA
                     GVNVLLTLPEGSEHEVLRRAGEAGIALQGLSRMRHPLAEVPNRDGIIIGFAAPAEHAF
                     AAAVEALCGVLRASGLGG"
     gene            5856..6326
                     /locus_tag="VFC01_07700"
     CDS             5856..6326
                     /locus_tag="VFC01_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN79556.1"
                     /translation="MTQTLEPKTTVDRVQIYKTSPELYDAMMVLSNAAAKDIDAELGE
                     LIKIRASQINHCAFCLDMHTRDARKHGVSEQKLDVLAAWEEAGQLFTERERAALALTE
                     AITELGNGHVPDDVYASAAAVFNDRELGQVIAMAVTINAWNRINVTIRLEPPRR"
     gene            6338..6799
                     /locus_tag="VFC01_07705"
     CDS             6338..6799
                     /locus_tag="VFC01_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN79557.1"
                     /translation="MTATVNKQRRINLNRVSPEVYEAMVVLNTAAAKDLDPDLAELIK
                     IRASQMNHCAFCIDMHTADARKRGIGDQKLALLPAWEEAEGIFTEQEQAALALTEEMT
                     DLTRRHVSDEVYARAAAAFSERELGQVIAMALTINAWNRIGVTTRLPIPKR"
     gene            6812..7540
                     /locus_tag="VFC01_07710"
     CDS             6812..7540
                     /locus_tag="VFC01_07710"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HZN79558.1"
                     /translation="MQTRSGAEIVVNGDVVCKLHRPGTNLRVLATRLRVAAESDSLLS
                     PLDVVPEPIGVRWRTRWPRVEVVAPQPECVPWAEAGRLLAALHGESTTLRLPHGWPQR
                     LRRMVRSTDDTVVRRAAAKLPPEVWRAGSPDRPTTLVHGDWHLGQLGRRAGPWVLIDV
                     DDLGVGDPAWDLARPAGFWGAGLIPDDDWTTFLDAYRDAGGPAVPAGDPWPVLEPFAR
                     AAVVQAAAHHPDDELLRAACERML"
     gene            complement(7542..8936)
                     /locus_tag="VFC01_07715"
     CDS             complement(7542..8936)
                     /locus_tag="VFC01_07715"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN79559.1"
                     /translation="MGVLRRRAKQLAGPASSEQATAEDSDAMYALGYRYSQQTDPPDL
                     EQAGDWYRRAADAGHVDAMYMLGYLNSQQTEPPDLDAARRWFERAADLGDSRAMNNLG
                     ALYAHQAQPPDLDAARAWYERASEAGNSDGMYGLAELHAALIEPADLNAAIHWYERAA
                     DAGHVAAMKWLGDFHANADPANLEAAQHFYQQAADAGDSDAMYGLGIVYLKSMDEPDL
                     PAARELFERAAEAGHRAAMYWLGMVWANYAEPPDLAKALQWFERSADAGHIGAMTALG
                     NLYAQVMQPPDLAAARHWYQRARAGGDRGAMHNLEVLYEDSTPTPPGWGTRPPSEEEM
                     DAMLEWLDGPGTALEDVFEASGPALDEVTDAFKHGDEAAVRAACTKVARLLTDELPAS
                     LPTPDPDLTRALQALIDDANEMSWAQRELADSPTPQQRETMQSRLGDIATSIHALLTI
                     FERNDAILESAGRG"
     gene            complement(8952..9281)
                     /locus_tag="VFC01_07720"
     CDS             complement(8952..9281)
                     /locus_tag="VFC01_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HZN79560.1"
                     /translation="MSIPPYEPPKAPPSPGAGNTLLCPKCAGVMKTYERNGIHLEQCD
                     TCRGIFLDFGELESLIQMENRFVQAAPPPPPPPPAQHGYDYGPGWGHRGNKHYRKQGF
                     GRLFFSS"
     gene            complement(9322..10716)
                     /locus_tag="VFC01_07725"
     CDS             complement(9322..10716)
                     /locus_tag="VFC01_07725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HZN79561.1"
                     /translation="MFEGAGDPELLAAMGEAQQAERAAFARQLLAVGEFTHRRMEAVY
                     AEHDFWCVDDWEAIAAEIGAELGISRGRASSQMGYGRALIERFPKLAEVFAAGAVDFR
                     VIAAAIFRTDLVHDNDALAVIDAQLAAKAPAWNKLSREKITELVDWLVLELDPDAVRV
                     AKQRDLDRHLEVGPGQHGMAEIWGEVRGPDAAVFDAKLNALAATVCPDDPRTTTQRRA
                     DALSPLSAGATHMACTCGKPDCPAAGTDVPAIPVVINVLAEAATVEGSSDKPGYQPGY
                     GAIPAATIQEMAKTASLRPVPSAKDLAAEPQYRPSAALARFVRCRDLTCRWPGCDQPA
                     IGCDIDHTVPYPHGPTHPSDTKCYCRIHHLFKTFHAGPAGWTEIQLPDGTISWTSPRG
                     RTYTTQPLGAQFFPQLGSPTETLILPNSPPLSPHRGLAMPKRKRTRTQDRAYRVQHER
                     AQNRARYEADPPPF"
     gene            complement(10796..11407)
                     /locus_tag="VFC01_07730"
     CDS             complement(10796..11407)
                     /locus_tag="VFC01_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN79562.1"
                     /translation="MAESRRTGRWRTGQQSKQRIVEVAREHFKQGYEQATVRRIAADA
                     GVDVAMVYYFFGNKEGLFTAAVIDIPEHPLHELAGLLDDGLDGIGARLVRRFVERWDE
                     GDTFEPLLTVWRSAADQPLARKLLHDTLAGPVAERVASEFGVDDAVLRVELVASHLMG
                     LAFARYQLRIEPIASVGVDELVAWIGPTVQRYLTGPTSVGPDS"
     gene            11499..12026
                     /locus_tag="VFC01_07735"
     CDS             11499..12026
                     /locus_tag="VFC01_07735"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZN79563.1"
                     /translation="MARRTLWWRHVLSVLLAPAMMTVFIPAAIVWLTGVDAPDLGTVP
                     GLLLVTAGIALIAGGLGMLVWTVVLFDRVGEGTLAIGSPVKLVVRGPYRHVRNPMMTG
                     VFCIQLGTAAVLASPWLLGWFAFFFTCVLIAIRTVEEPHLRRRFGADYDEYRRHVPRW
                     IPRITAWEPSRAYTQ"
     gene            complement(12014..12844)
                     /locus_tag="VFC01_07740"
     CDS             complement(12014..12844)
                     /locus_tag="VFC01_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN79564.1"
                     /translation="MTFTDAAAQSRTMSRAWGDLVAGFSKRELWLHLGWQDIKQRYRR
                     SVLGPFWITIATGATAVAMGLLYSKLFKLPLAEHLPYVTLGLIIWNLINASILEGAEV
                     FVANEGLIKQLPTPLSVHVYRLVWRQMILFAHNIIIFVVIAIIYPKPWKWTDLAVIPA
                     LGLIVLNCVWVSLCFGILATRYRDISPLLFSLVQLLFFMTPIIWNESTLQQQGAGAYA
                     KIVELNPLLHYLDIVRAPLLGADQELRHWLVVLALTVIGWIMAAFAMRQYRARVPYWV
                     "
     gene            complement(12841..13740)
                     /locus_tag="VFC01_07745"
     CDS             complement(12841..13740)
                     /locus_tag="VFC01_07745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN79565.1"
                     /translation="MTESVCAVVVTHRRPDELAKSLDSVCAQTRAPDHLVVVDNDDDA
                     RVRDIVAGQPVATTYLGSRRNLGGAGGFALGMLHALSLGADWVWLADDDGRPLDSEVL
                     GTLLTCASRHGLAAVSPMVCDMADPQRLAFPLRRGLVWRRRVSELRTGTEDLLPGIAS
                     LFNGALFRASTLEAVGVPDLRLFVRGDETELHRRLVRSGLPFGTCLNAVYLHPQGSDE
                     FKPILGGRMHTQYPDDATKRFFTYRNRGYLQSMPGMRKLVFQEWMRFGWYFLVTRRDP
                     AGLAEWIRLRRLGRREKFERPSQ"
     gene            complement(13733..14614)
                     /locus_tag="VFC01_07750"
     CDS             complement(13733..14614)
                     /locus_tag="VFC01_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN79566.1"
                     /translation="MSLPHIETHNAWVEFPIFDAKTRSLKKAFLGKAGGAIGRNESNV
                     VVIEALRDITMSLELGDRVGLVGHNGAGKSTLLRLLSGIYEPTRGVASVTGRVAPVFD
                     LGVGMDPEISGFENIIIRGLFLGQTRKQMLAKVDEIAEFTELGDYLSMPLRTYSTGMR
                     VRLAMGVVTSIDPEILLLDEGIGAVDAEFLKKAQTRLQSLVERSGILVFASHSNEFLA
                     RLCKTAMWIDHGTIKMTGGIEDVVRAYEGEDAARHVREVLEEHRGDWSPTDGSSADWP
                     ATAMSASREEQTALPDD"
     gene            complement(14621..15121)
                     /locus_tag="VFC01_07755"
     CDS             complement(14621..15121)
                     /locus_tag="VFC01_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial proteasome activator family protein"
                     /protein_id="HZN79567.1"
                     /translation="MTANTDDDNIEIIGSDASAALDADADGKSLTDLVEQPAKVMRIG
                     TMIKQLLEEVRAAPLDDASRNRLREIHRTSIRELEDGLAPELRDELERLTLPFTEDAV
                     PSDAELRIAQAQLVGWLEGLFHGIQTALFAQQMAARQQLEQMRQLPPGVAAPGQRGGP
                     GTGQYL"
     gene            complement(15118..17067)
                     /locus_tag="VFC01_07760"
     CDS             complement(15118..17067)
                     /locus_tag="VFC01_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6541 family protein"
                     /protein_id="HZN79568.1"
                     /translation="MSLGFGVLIALFLLVIPGAIFARVGRLTWPAAVAVGPALTYGVV
                     ALAIVPFGALGVPWNGWTALFALAVATAAAWCLRIALARVRDVDAETRAVARGPALVL
                     AAGVLLGALLIGLAAIRGMPNWQSIPSNWDSVWHANTIRFILDTGQASPTHMGELRNV
                     ETREALYYPSTFHAVAAVFSQLTGAAPTTAYTLNSVAASVWLFPVSAATLTWHLLRTR
                     TDEWRTAGAAAAAAALSGSFTALPYIEFDTASMPNLAAYGVAVPTMVLVVSALRHRDR
                     IPLAVLALLGVFSVHITGGVVTVLFVAAWWLFDALWHPVRGRVADFVNLLLIAIPTVA
                     LLLPQFLGVLRQAEIIVGHAFVTHEGKKRGLIDAVVQHTRHLNDFPIQWVLIILAAVG
                     GVVLIYRRVWWPLAAWFLLVVSIVHSSAPFGGPIGELTGKFSDLFYSDPRRLSAVVTM
                     LLTAAVGIGVFTLASVVVAGGRRLVGRGSPRAWNAATAAIVVAVSIGIGLAYLPRAQF
                     LFGEKYDRVIVDSKDLEAWAYLATLPGARDTLIGDANTDGTAWMYAAAGLHPLWTHYD
                     YPVQQGPGYHRFIFWAYADDADTDKRVAEAVKALNIRYVVTSTPVVRGFVMPDGLVSL
                     DRSRSWEKIYDNGEARIYEWRGEEK"
     gene            17193..18389
                     /locus_tag="VFC01_07765"
     CDS             17193..18389
                     /locus_tag="VFC01_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HZN79569.1"
                     /translation="MAYDVARVRGLHPSLGDGWVHFDAQNGMLLPDSVGRAVSTAFRG
                     SMPTPIGPHPSAQRSAAVLEAARQAIADLVNADPRGVVLGADRAVLLTSLADASSSRV
                     GLGYEVVVTRLDDEANIAPWLRAANRYGAKVKWAEVDIETGELPTWQWEGLITRSTRL
                     AAIASASSTLGAVTDLRAVTKLVHEGGGLVVVDHSAGAPYRLIDIDEIDADVVALNAV
                     AWGGPPIGALVFRDPSMINSFSSVSLNPYATGPARLEVGTHQFGYLAGVVASIEYLAG
                     LDESAQGSRRERLSVSMQSASTYLNRVFDYLLVSLRSLSAVMVIGRPDVHIPVLSFAV
                     SDVPAERVVQRLADNGVLAISNVSSRVLDVLGVNDVGGAVTVGLAHYTTTAEVDQLVR
                     ALASLG"
     gene            complement(18386..19360)
                     /locus_tag="VFC01_07770"
     CDS             complement(18386..19360)
                     /locus_tag="VFC01_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HZN79570.1"
                     /translation="MRAIVAEAADQLTWQEIPDLEPGDSEVLIKVSAAGVNRADLLQA
                     AGNYPPPPGASQILGLEVSGTIAKVGDEVVDWVVGQQVCALLAGGGYAEFVAVPAGQV
                     MPIPADVPLHHAAALPEVACTVWSNLMMTAHLSAGRLLLIHGGASGIGTHGIQVARAL
                     GARVAVTAGAQNKLDLCSELGAEITINYRDEDFVERVRSAGGADVILDIMGAKYLDRN
                     VDALADGGRLIIIGLQGGVKAELNIGKLLGKRAGVIATALRSRPVSGAGSKSDIVAEV
                     VANLWPMIADGQVRPIVGAEFPIEQAGAAHELLNSGEVSGKILLRVAD"
     gene            19439..19951
                     /locus_tag="VFC01_07775"
     CDS             19439..19951
                     /locus_tag="VFC01_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN79571.1"
                     /translation="MEGIIAGRTASDMPGLDIAEQRSWQNFLDSALRMYATLNRSLVD
                     AHHLTLNDVRLLDILDKSATGSARMGDLADGLMSLPSRVTRQIRRLELQGLVRRGASP
                     DDGRGVLASITDEGRVAVTEAMLTYGQGVRAHFLGRLSRPQIAAMGENCRRISVGLKG
                     TPSAAKVGRV"
     gene            19971..20057
                     /locus_tag="VFC01_07780"
     tRNA            19971..20057
                     /locus_tag="VFC01_07780"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20005..20007,aa:Ser,seq:tga)
     gene            complement(20062..>20127)
                     /locus_tag="VFC01_07785"
     CDS             complement(20062..>20127)
                     /locus_tag="VFC01_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79572.1"
                     /translation="KDLTEGITATFAKRPPQFTGE"
BASE COUNT         3434 a         6519 c         6777 g         3397 t
ORIGIN      
        1 cgtcgaacag cgcgttgtcc agctcaacgt ggtagcggcg gacgttgggc tggttggggt
       61 acacgtccga cgccagccgc agggtgtagg tgtcgctcgc gccgcgccgc atcaagaaca
      121 ccgtcggcgc cttccacggc agtttgtcca gcgccgcgat gaactggtcg gccgtggtca
      181 gcgtggccca gctttcgatc gcggcggccc gctggtcgaa ctgcgcgagc ggatttgcgt
      241 agtgggacgt cagaccctgg aagccgtagt acgggtagta ggacaggaag ctgtagtccg
      301 cagtcagcag aaccgtttcg tcgcgcggca ggccggtggt ttcgcggatc ttggtgtcga
      361 tctcgcggta gtactgctcg gcgccgggcg gccgccggtc ggcgcgttgg ccgtagccgt
      421 cggtatcggt gtaggcgacg acgatgtccg agcgcaacac gtccggaatg tcctggctga
      481 aggtcagtgc gccgatcgcg ccgacggtgg cggcggccgc gacgacgcgt cttgcattct
      541 cgggcctgac gcgggcagca agcgcacggg tgacttcgat gaacccgaac gcgccggccg
      601 cggtgagcag gatcgtcagg gtgggctcaa gccggaacga cagcagcgtg gtgccgacca
      661 gcgtcctgag catcgacaac agcgaccagg cgtaaacagc cagcaccgcc acggtcaacg
      721 cgcccgcgcg cgtggacgag cgggcgcgcc acaccagcca cagcgtgccg agcatgcaca
      781 acgcacccag cagggtgaag cgcagcattg gaaattccag cgatgcgccc gcctccggca
      841 gatagtgctg ggcggtgccc gagtcggcgg gctgaccgtg tagcgctgcc cacagataag
      901 gaccccagcc gagcagcgcg atggcgcccg agatcaccgc gatgacgacg agccgcagca
      961 acggctcgag acgtctgcgc gcgaccgcga gcagcacccc catgaccgcc agcgtgaacg
     1021 cggcgtagcc gaacagcagc gtgtagaaca gcgcagcgat tccgaggaag atgccgacgc
     1081 cgatgactgc cgcccagccg ccgtttcggg tgccgccgcg caaaccggac cacgcgagca
     1141 cgaataccgc aggcagcagc acggcgatga ttgcggcgta cggctcggtg ggtgagtagg
     1201 ccagtgcggc ggcggcggtg gcggtcgaga cgatcagcgc gtactcgaag cgaaccatgc
     1261 tggcccacaa cacgaacgcc agcaccacgg cgatcgtgat ggagatgatc gcccacggct
     1321 tgaacacctc ccacgcgggc atgcccgtca gtgcggcgag ccggccaccg agccagaacc
     1381 agcccggcgg atagaacggc ggcagaccgt agtaggtcat gtcgtgtggg ccggcggtgt
     1441 cggtgagccg ggtcaggtac tcggtgcgga actgctggtc gacggagatg ccgtacaggt
     1501 aaagcttggt cgcgccgagc ggcatggcca gcgtgacgac cgcgaacgcg gacaagaaga
     1561 caaccgccgc ggccttcgcg agcaggcggc gtccgcgacg ccaaatccag ccagtcgcca
     1621 agatgcctgc caggcaaccg aattggccga cggtggtcag cgcgtgcagc tggttcgacg
     1681 agttgtacgc cggccactca acacgggaga tcgcgacaag cgagaccacc gcaacgacca
     1741 ccgccacggc gaccgccacc gccatctggc cgatcacggc cagtgcgcca cgcatcgcta
     1801 gatggggagc ttgcggaaga tgggccgcgg tatgtgtcgc aacaccatca tcacgtacct
     1861 gaacgccccc ggcgcccata ccagttcctt gcccttggcc gatgccgtta ccgccaactc
     1921 ggcaacgtct tccttgtcga cggtgaacgg cgcttccttg gcgcccgtgg acttccagtg
     1981 ctcgatagtc gtcgtggtgc gcacctgacc cggcctgatc accagcaccc gaactccgta
     2041 ctcgcgcaac gcttccccga ggccgaggta gaagccgtcg agtccggcct tggtggatcc
     2101 gtagacgaag ttggaccgtc ggacccgctc gccggcagcg gagctcatgg cgatgatctg
     2161 gccgaagccc tgcgctttca tcttctcgcc gagcagcacc ccgaccgaaa ccgctgcggt
     2221 gtagttgatc tcggcgatct gcacggcctt gcgctggttc tgccatagct cttcggcgtt
     2281 gccgagtagg ccgaatgcga cgatcgccac gtcgacatcg ccaccggcga aggcagcgtc
     2341 gatgaccttt ggatggctgt ctgtgtcgac ggcatcgaag tcgatcacct cgacggactt
     2401 ggcgcccgcc gccttcatct gctcgacggc ggtgtcgcgt cgcggatggt cggggagctc
     2461 ggccagcacg atgcgcgccg atgcgttgcg cagatagcgc tcgcagatgg cgagcccgat
     2521 ttcggacgtg ccgccgagca gcagaatggt ctggggattg cctacagcgt cgagcaccat
     2581 ctagagcagc tccaagcgtc gggccatgtc ggaggcgaac acgccgttgg gatccacctt
     2641 gcggcgcacg gcaatccact cgtcgattcg cgggtacatg gcgtggaagg tctcggcggt
     2701 cgtgcggttg tccttggctg tgtagagcct gccaccgaac tccaacaccc ggcggtcgag
     2761 ctcgttgagg aactcgttga ggccgggctt gatcgggaag tcgacgcaga cgttccagcc
     2821 ggggatcgga aaactcagcg gcgcttggtt tcccgggccg aacagtttga acacgttgag
     2881 gaacgagtaa tgccccgact gctggatgtc gtacatgatg cccttgaagg gctcgacggc
     2941 ctccgtgggc acgacgaatt ggtattgacc gaagcccgca gggccgtagg cgcggttcca
     3001 ctccccgaac atgtcgagcg ggtgatagaa ctgcgtcaga ttctggatct tgccgcggta
     3061 ggtacctgac ttgcggtacc agagctcgcc gatcggcatg aatgtgtact tgcccaccag
     3121 catgccgttg ggcacgatat cgggaatcgt aagtagctgc ggcgcatcga atttcagcgg
     3181 attgcgctgc aacttcttcg gcagctggtc gattgtggcc agcgaaccgc gggagatcgc
     3241 ggcacggccg agcttgggcg gcgcgctgat cgcgtcgaac cacgccgacg agtacgtgta
     3301 attgtcctcg ctgccgtcgc tgtggaatgc gatcgtctcg tcgagactgg cggtcacgtc
     3361 gccgtcggcg atgaaatagg ccgtctcggt gggcgtcatc tcgatgatcg cgcgcaggat
     3421 gatgccggtg agcccgttgc cgccgaccgt cgcccagaac aactcggact ccggaccgtc
     3481 cggggtgagc tgacgaatct cgccgtcggc ggtcagcagg tccattgacc gcacgtgatt
     3541 gccgaagctg cctgcgctgt ggtggttctt gccgtggatg tcgcaggcga tcgcgccgcc
     3601 gatggtgacc tgccgggtgc cgggcagcac tggcacccac agcccgcacg gcagggcggc
     3661 gcgcatcagt tggtccagat tgacacccgc gtcgacatcc accaggtggc tgtcggcgtc
     3721 gatcgagtgg atcttgttca gcgcgttcat gtcgatgacg aggccgccgc cgttctgggc
     3781 gttgtcgccg tacgagcggc cgagtccgcg cgcgatcacg ccccggctgt cggcctgcga
     3841 cacggccttg gcgatcacct cggggtccgg ggtcgacagc acctgcgcga cactcggcgc
     3901 ggtgcgggcc cagccggtga ggcgctgggt actggtctca aacatcgtga cgagggtacc
     3961 gcctgaccct tttggctcag cgcagccgga aaatgaccgc ccgctggacg atgaagttga
     4021 tcacagtcgc ggtgccctgg gcgataacga acgccaccgg cacggcccac gccttgtagt
     4081 cgaacagatg caggcagtaa tggttgaggc cgacctgcac ggcgaacgta attccgtaca
     4141 gcgccatgac cgcgatgaac cgggcgatgc catgttctgc ttggaacgtc catcgcaggt
     4201 tgatcaggta tgccgtaatg gtgccgaaga tgaagctgat cgccttggcc aggtcgacct
     4261 gcacgcccac cgctttgtac agcaggacgt agaggccgaa gtcaacgatc gccgcgaaac
     4321 cgccggtgac gatgaaccgc cacgcctggg ttttgagagt caggttcggc gacatcgggg
     4381 gctcggccac ccgggcagct tagccaccca ggccgctggc cctcagcacc ccgcacaggg
     4441 cctcgacggc cgcggcaaac gcatgctcgg cgggggccgc gaaaccgatg atgatgccgt
     4501 cgcggttggg gacctcggcc agcggatggc gcatccgtga caggccctgc agcgcgatac
     4561 cggcctcacc cgcccgtcgc agcacctcgt gctcgctgcc ctcgggcagg gtcagcagca
     4621 cattcacccc cgcggccaga ccgctgatgc cgacgtcgaa tccggcgagc gcgtcgatga
     4681 gggtgtcgcg gcgtcgccgg taccgtccgc gcatcctccg gatgtggcgg tcgtactgac
     4741 cgctggcgat gaactcggcc atcgtgagct ggttgatggc gtcgacgtag aactgtgcgc
     4801 cacccgcggc ggcgatcacg gggtcgatga gctcgtcggg cagcgccgtc caaccgagcc
     4861 gcaaggtctg cgcgaggctc ttgctggtcg acccgaggta ggcaacgcgg tcgggacaca
     4921 gcgcctgcag cgcgccgacg ggttgacggt cgtaccggaa ttccccgtcg tagtcgtcgt
     4981 cgaacacgta tccgcccgtg cgctgcgccc aatcgatgac ggcagtgcgc cgcgacgggt
     5041 gcaacggcat gccctgcggg ttgtggtggg ccggcgtgag cagcacggcg ggcacgtcca
     5101 actcgtcgag gtcgctgatc accgcgccct gttcgtcgag gccgatcggg acggtcgatg
     5161 tccccaacgc gtcgatggcg tcgcgaaaga tgaagagccc gtaggcctcg acggcgatcg
     5221 ggccgccgac ggcccgtgtc aacagttcga ccgcgtgccg gacgccggcg caaatcacga
     5281 tcgactccgg cgacgtgcgc acgccgcgcg cccgggcaag gtattcggcg agcgcattgc
     5341 gaagttcggg ccggccacgt ggatcgccca tccgcagcgc ctcggtcggc gcatcggcga
     5401 gggcgcggcg ggtggcggcc acccatgcgg tgcgaggaaa ctcggacacg tccggagagc
     5461 cgggcgacag gttgtgcgtc ggaacgacgc ggacgccgcg gggtgttgcg ggtatgtccg
     5521 cgccgctgac gttcaccacc caggtgcccg cgcctcggcg cgacgcgagc catccctcgg
     5581 cgacgagttc ggcgtaggcc tcggccacag tgttgcgggc caatccgaga tcggtggcca
     5641 gggtgcgaga gggcggcagc atggtgccgg gtgccaggcg gcccgatcgc accgcgtcgc
     5701 gcagagcact gagcagcagg tcacgggccc cgcgagcgcc cggtgtgatg gtctggcgca
     5761 gatcgaggtg caggtcgcgg gtccccgaat tggcccatga atccatagga atattgaacc
     5821 atgtagatgg gtctttcggt attagcgttg acggcatgac acagacactg gaaccaaaga
     5881 ccaccgtcga ccgggtgcag atctacaaga cgtcgcccga gttgtacgac gccatgatgg
     5941 tgctgagcaa tgcggccgcc aaggacatcg acgccgaact gggcgagctg atcaagattc
     6001 gcgcctcgca gatcaaccac tgcgcgttct gcctcgacat gcacacgcgt gacgcccgca
     6061 agcacggcgt cagcgagcag aagctcgacg tcctcgcagc gtgggaagaa gccgggcagc
     6121 tgttcaccga acgtgagcgg gcggcgttgg cgctgacgga ggccatcacc gagctcggca
     6181 acggccacgt ccccgacgac gtctatgcaa gcgccgcagc cgttttcaat gaccgtgagc
     6241 tgggtcaggt catcgcgatg gcggtcacca tcaatgcgtg gaaccgtatc aacgtgacga
     6301 tccgcctcga accgccgcgc cgttaggaag agccgacatg actgcaaccg tgaacaagca
     6361 acgccgcatc aacctgaacc gggtatcgcc cgaggtttat gaggcgatgg tggtgttgaa
     6421 caccgccgcg gccaaggacc tcgacccgga cctggccgag ttgatcaaga tccgtgcgtc
     6481 gcagatgaac cactgcgcgt tctgcatcga catgcacacc gccgacgccc gcaagcgcgg
     6541 catcggtgac cagaagttgg cgctgctgcc cgcgtgggaa gaggccgagg gcatcttcac
     6601 cgagcaggag caggcggcgc tcgccttgac cgaggagatg acggatctca ctcgccgcca
     6661 tgtttccgac gaggtgtatg cccgtgcggc ggcggcgttc tccgagcgcg agctcggtca
     6721 ggtcatcgcg atggcgctga ccatcaacgc ctggaaccgg attggcgtga caacgcggct
     6781 gccgataccc aagcgctaat accgtcgagg gatgcagacc cggtccggtg cggagatcgt
     6841 cgtcaacggc gacgtcgtgt gcaagctgca ccggccgggc accaacctac gcgtcctggc
     6901 cacccggctc cgcgtcgcgg cggagtcgga ttcgctgtta tcgccgttgg acgtcgtacc
     6961 ggagccgata ggcgtgcgat ggcgtacccg gtggccgcgc gtggaagtcg ttgcgccaca
     7021 acccgaatgt gtgccgtggg ccgaagcagg gcggctgctg gcggcactgc acggcgaatc
     7081 gacgactttg cggctgccac atggctggcc gcagcggctt aggcggatgg tgcgatccac
     7141 cgacgacacc gtggtgcggc gtgcggcggc caagctacca cccgaggtgt ggcgggccgg
     7201 ctcaccggat cggccgacga cgctggtgca cggcgactgg catctcggcc agctgggccg
     7261 ccgagccggg ccttgggtgc tcatcgacgt cgacgatctc ggtgtcggtg acccggcctg
     7321 ggacctggcc cgccccgcgg gcttctgggg ggctggcctc attcccgatg acgactggac
     7381 cacgttcctc gacgcatacc gtgacgctgg cggaccggcc gtgccagctg gtgatccgtg
     7441 gccggtgctc gagccgttcg cgcgcgctgc ggtggtacag gccgctgcgc accatccgga
     7501 tgacgaactg ttgcgcgccg cctgcgagcg gatgctttag gtcagccccg acccgccgac
     7561 tcgagaatcg cgtcgttgcg ttcgaagatt gtaagcagtg cgtgaatgct tgtcgcgata
     7621 tcgccaagac ggctctgcat cgtttcccgt tgctgaggtg tcggcgagtc ggccagttcg
     7681 cgctgggccc agctcatctc gttggcgtcg tcgatcagtg cttgcagtgc ccgagtgagg
     7741 tcgggatcgg gtgttggtag tgaagctgga agctcgtcgg tgagcaggcg agcgaccttg
     7801 gtgcatgcgg ctctgactgc cgcctcgtcc ccatgtttga aagcatcggt gacttcatca
     7861 agcgcaggac ccgacgcctc aaacacgtct tcgagggcgg tgcccggccc gtcgagccat
     7921 tcgagcatcg catccatttc ttcctcgctc ggcggccgcg tgccccagcc cggtggcgtt
     7981 ggtgtcgagt cctcgtagag cacctcaagg ttgtgcatcg cgccgcggtc accgccggcg
     8041 cgggcgcgct gataccagtg acgggccgca gcgagatccg gcggctgcat cacctgtgcg
     8101 tacaggtttc cgagtgccgt catggcgccg atgtggccgg cgtcggcgga acgctcgaac
     8161 cactggagag ccttagcgag gtcaggtggc tcggcgtagt tcgcccacac cattccgagc
     8221 cagtacatgg cggcccggtg tccggcctcg gctgctcgct caaagagctc gcgcgcggca
     8281 ggcaggtccg gctcatccat cgacttcaga tagacgatcc cgaggccgta catggcgtca
     8341 gagtcacctg cgtcggcggc ttgctggtaa aagtgttgcg ccgcttcgag attcgccggg
     8401 tcggcgttcg cgtggaagtc tcctagccat ttcattgcgg cgacgtgtcc agcgtcggct
     8461 gcgcgctcat accagtggat cgctgcattg aggtccgccg gctcgatcaa ggccgcgtgc
     8521 agctccgcga ggccgtacat gccgtcgctg ttgcctgcct cggaagcacg ctcgtaccaa
     8581 gcgcgggccg cgtcgagatc cggtggctgc gcctggtgcg cgtacagtgc gccaaggttg
     8641 ttcatggcgc ggctgtcgcc gaggtcggcg gcgcgttcga accagcggcg cgcggcgtcg
     8701 agatcgggtg gctctgtctg ctgcgagttc aggtacccga gcatgtacat ggcgtcgacg
     8761 tgcccggcgt cggcagcacg gcggtaccag tcgccagcct gctcaagatc cggtggatcc
     8821 gtctgctgtg agtaccgata cccaagggcg tacatggcgt cgctgtcctc ggcggtggcc
     8881 tgctccgatg aggcggggcc cgcaagttgt ttcgcgcgac ggcgcagtac gcccattgca
     8941 gcgaagccta gctagctaga gaagaacagc ctgccgaatc cctgcttgcg gtagtgcttg
     9001 ttgccgcgat gaccccaacc ggggccgtag tcgtagccat gttgagccgg cggaggcgga
     9061 ggcggaggcg gcgccgcctg cacgaaccgg ttctccatct ggatgagcga ctccagctca
     9121 ccgaagtcca ggaagatgcc tcggcaggta tcgcactgct cgaggtggat gccattgcgc
     9181 tcgtacgtct tcatgacgcc ggcgcacttg ggacacagca gcgtgttccc cgcacccggt
     9241 gacggagggg ccttcggtgg ttcgtatggc gggatgctca tattccgaca acggccgagc
     9301 accctgcgaa gtgcctggga actaaaaggg tggtgggtcg gcttcgtagc gcgcgcggtt
     9361 ttgggcgcgt tcgtgttgca cccggtaggc gcggtcttgg gtgcgggtgc gttttcgctt
     9421 gggcatggcc aacccgcgat gcggactgag tggtgggctg ttgggcagga tcaatgtttc
     9481 ggtgggcgac ccgagttggg ggaagaactg ggcgccgagg ggttgggtgg tgtaggtgcg
     9541 tccgcgtggt gaggtccagc tgatggtgcc gtcgggtagt tggatctcgg tccagccggc
     9601 cggtccggcg tgaaaggttt tgaacaggtg atggattcgg cagtagcact tggtgtctga
     9661 cgggtgggtg ggcccgtgcg ggtagggcac ggtgtggtcg atgtcgcagc cgatggcggg
     9721 ttggtcgcag cccggccagc gacaggtcag atcccgacac cgcacaaacc gcgccagtgc
     9781 tgccgagggc cggtactgcg gttcggcggc cagatccttc gcgctgggga cggggcgcag
     9841 ggaggcggtc ttggccatct cctgaatggt cgcggccggg atggcgccat agccgggctg
     9901 atatcccggc ttgtcactgc tgccttcgac ggtggcggcc tcggccagca cgttgatcac
     9961 caccggtatg gccggcacgt cggtgcccgc ggcggggcag tccggcttcc cgcacgtgca
    10021 cgccatatgg gtggcacctg cggatagtgg gctcagggcg tcggcgcggc gctgggtggt
    10081 ggtgcgcgga tcatccgggc acacggtggc ggccagcgcg ttgagtttgg cgtcaaagac
    10141 ggccgcatcc gggccgcgga cctcgcccca gatctcggcc atcccgtgtt ggcccggccc
    10201 gacttcgagg tggcggtcga ggtcgcgctg tttggcgacg cgcaccgcgt cggggtcaag
    10261 ctcgaggacc agccagtcga ccagttcggt gatcttctcg cgggacagtt tgttccaggc
    10321 cggcgccttg gcggccagct gcgcgtcgat gaccgcgagc gcgtcgttgt cgtggacgag
    10381 gtcggtgcga aagatggctg cggcgatgac ccgaaagtcc accgcaccgg ccgcaaacac
    10441 ctcggccagt ttggggaagc gttcgatcag ggccctgccg tagcccattt gtgaagacgc
    10501 gcgcccgcgg ctgatgccca actcggcgcc gatctcggcg gcgatggcct cccagtcatc
    10561 gacacaccag aagtcatgtt cggcatacac cgcctccatg cggcgatggg tgaactcgcc
    10621 gaccgccagc agttggcgcg cgaacgcggc ccgctcggcc tgctgggctt cacccatggc
    10681 cgcgagcaac tccggatcac cagcgccttc gaacacatgt ttgattcttc cggccggccc
    10741 gacatcccac caccgccaaa cgccagcctg tggatgaatt caggactggg gataactacg
    10801 agtcagggcc cacggatgtc ggacccgtga ggtaccgctg cacggtcggt ccgatccacg
    10861 cgacaagctc gtcgacgcca acagacgcga tgggttcgat ccgtagttga taacgcgcga
    10921 acgccagccc catgaggtgc gaggcgacca actcgacccg caacaccgcg tcgtcgacgc
    10981 cgaattctga agccacccgc tcggcgacgg gcccggccag ggtgtcgtgc agcagcttgc
    11041 gggccagcgg ctgatcggca gccgatcgcc acactgtcag taggggctcg aaggtgtcgc
    11101 cctcgtccca gcgctcgacg aagcgccgga cgagacgtgc gcctatccca tccaagccgt
    11161 cgtcgagcag gccggccagc tcgtgcagcg gatgctccgg aatgtcgatc accgcagcgg
    11221 tgaacaggcc ttccttgttt ccgaagaagt agtagaccat cgcgacgtcg acgccggcgt
    11281 ctgccgcgat ccggcgcacg gtggcctgct cgtagccctg cttgaaatgc tcgcgcgcga
    11341 cttcgacgat gcgctgcttg ctctgctgcc ccgtgcgcca gcggccggtt ctccggctct
    11401 ccgccatgcc cgaaagcgta cttcaacacc cgttgatgtt tgttcgcggc aagcgtagcg
    11461 tcgttcttga acaaccgttg aagaaggagg aatacacgat ggctcgacgg acactctggt
    11521 ggcggcacgt gctgtcagtg ctgctggcgc cggcgatgat gacggtgttc attccggccg
    11581 cgatagtgtg gctcactggt gttgatgcac ccgatctcgg caccgtgccg ggtctgctac
    11641 tcgtcaccgc cggcattgcg ctgatcgcgg gcggcctcgg catgctggtg tggaccgtgg
    11701 tgctgttcga ccgcgtcggc gagggcacac tcgcgatcgg atcacccgtc aaactggttg
    11761 tccgcggccc gtatcggcac gtccgcaacc cgatgatgac gggcgtgttc tgcattcagc
    11821 tcggcacggc agcggtgctg gcatcgccgt ggctgctcgg ctggttcgcg ttcttcttca
    11881 cgtgcgtgct catcgcgatc cgcacggtcg aggaaccgca tctccgccgg cgcttcggtg
    11941 ccgattacga cgagtaccgt cgccacgttc cgcgctggat tccgcgaatc acggcatggg
    12001 agcccagcag ggcttacacc caatagggca cgcgcgcccg atattgacgc atcgcgaacg
    12061 ccgccatgat ccaaccgatc acagtcagcg ccagcactac cagccagtgc cgcaactcct
    12121 gatcggcgcc aagcagcggc gcccgcacga tgtcgaggta atgcagcagt gggttgagtt
    12181 cgacgatctt cgcgtatgcc cccgcgccct gctgctgcag cgtcgactcg ttccaaatga
    12241 tgggtgtcat gaagaacagc agctgcacga gggaaaacag cagcgggctg atgtcgcggt
    12301 accgagtggc caggatgccg aaacacagtg acacccatac acagttgagc acgatcaggc
    12361 ccagcgccgg gatcaccgcc aggtcggtcc acttccacgg cttgggatag atgatcgcga
    12421 tgaccacaaa gatgatgatg ttgtgcgcga acaggatcat ctgacgccac accagtcggt
    12481 agacgtgcac cgacagcggc gtcggaagtt gtttgatgag gccctcattc gcgacgaaca
    12541 cctcggcgcc ctcgaggatc gacgcgttga tcaggttcca gatgatcagg ccgagcgtga
    12601 cgtacggcag gtgttcggcg agcggcagct tgaacagctt cgagtacagc agccccatgg
    12661 cgaccgcggt agcgcccgtc gcgatcgtga tccagaacgg gccgagcacg gaacggcggt
    12721 agcgctgctt gatgtcctgc cagcccaggt gcagccacag ctcgcgctta ctgaagccgg
    12781 cgacgaggtc gccccacgcg cggctcatcg tgcgtgactg cgcggccgcg tcggtgaacg
    12841 tcattgtgaa ggcctctcga atttctcgcg tcggcccaag cgccgcaacc gaatccattc
    12901 cgccagcccg gcggggtcac gacgtgtcac caagaaatac cagccgaacc gcatccattc
    12961 ctggaacacc agtttccgca tgcccggcat ggactgcaga tagccgcggt tgcggtaggt
    13021 gaagaaccgc ttggtggcgt cgtcggggta ctgcgtatgc atccgcccgc cgaggatcgg
    13081 cttgaactcg tcgctgccct gcggatgcag atagaccgcg ttcaggcagg taccgaacgg
    13141 cagccccgag cgcaccagtc gtcggtgcag ttcggtttcg tcaccacgca cgaacagccg
    13201 aaggtccggc actccgaccg cctcaagcgt cgacgcccga aacagtgccc cgttgaacag
    13261 cgatgcgatc cccggcagca gatcttcagt gcccgtgcgc agttcgctca ctcgccgccg
    13321 ccacaccagg ccccggcgca acggaaacgc cagtcgctgc ggatccgcca tatcgcacac
    13381 catcggcgac accgccgcca gtccgtgccg cgaagcacag gtcaacagtg tgccaagcac
    13441 ctcggaatcc agtggcctgc cgtcgtcgtc ggccagccac acccagtccg ctccgagcga
    13501 taaagcatgc agcatgccca acgcgaaacc gcctgcacca ccgaggtttc ggcgcgaccc
    13561 gagataggtg gtcgcgaccg gctggccggc cacgatgtcg cgcacccgtg cgtcgtcgtc
    13621 gttgtcgacg accaccagat gatcgggggc gcgggtctgt gcacacacgg aatccagcga
    13681 cttggccagt tcgtcgggac gccgatgcgt gaccaccacc gcgcataccg actcagtcat
    13741 cgggcaatgc tgtctgctct tcgcgcgagg cgctcatcgc cgttgcaggc cagtcggcgc
    13801 tggagccgtc tgtcggcgac caatcgcctc ggtgctcctc gaggacctct cgcacgtggc
    13861 gcgccgcgtc ctcgccctcg tatgcgcgca cgacgtcctc gatgccgccg gtcatcttga
    13921 tcgtgccgtg gtcgatccac atcgcggtct tgcacagccg ggcgagaaac tcgttggaat
    13981 ggcttgcgaa caccaggatc ccggatcgct cgacgaggct ctgcagccgt gtctgcgcct
    14041 tcttcaggaa ctcggcgtcc accgcgccga tgccctcgtc aagcagcagg atctccgggt
    14101 cgatgctggt gaccacaccc atggccaacc gcacccgcat cccggttgag tacgtacgca
    14161 acggcatcga caggtagtcg ccaagttcgg tgaactccgc gatctcatcc accttggcca
    14221 gcatctgctt tcgcgtctga ccgagaaaca gcccgcggat gatgatgttc tcgaatccag
    14281 agatctccgg atccatcccg acgccgaggt cgaacacagg ggccacccgg ccggtcaccg
    14341 aagccacgcc gcgggtgggc tcgtagatcc cggacagcag ccgcagcagt gtcgacttcc
    14401 ccgcgccgtt gtgcccgacg agcccgaccc gatcgccgag ttcgagagac atagtgatgt
    14461 cccgcaacgc ctcgatgacc acgacgttgg attcgttgcg tccgatcgcg ccgccggcct
    14521 tgccgaggaa cgccttcttc agcgagcgcg tcttcgcgtc gaagatcgga aactccaccc
    14581 acgcgttgtg ggtctcgatg tgcggaaggg acaacggaac ctacaggtac tgaccggtcc
    14641 ccgggccgcc gcgctgtccg ggcgccgcca caccgggcgg taactgccgc atctgctcca
    14701 gctgctgacg ggccgccatc tgctgggcga acagcgcggt ctggatgccg tggaacaggc
    14761 cctcgagcca gccgaccaac tgagcctggg caatccgcaa ttcggcgtcc gacggaacgg
    14821 cgtcctcggt gaacggcagc gtcagccgct cgagttcgtc acgcagttcc ggcgccaacc
    14881 catcctcgag ctcgcggatg ctggtgcggt ggatctcacg cagccggttg cggctcgcgt
    14941 cgtccagcgg agcggcgcgc acctcctcca gcagctgctt gatcatggtg ccgatccgca
    15001 tcaccttcgc cggctgctcg accagatcgg tcaacgactt gccatcggcg tccgcgtcga
    15061 gagccgcgct cgcgtcgctg ccgatgatct cgatgttgtc gtcgtcggtg ttggcagtca
    15121 tttttcctct ccgcgccatt cgtagatgcg ggcctcgccg ttgtcgtaga tcttctccca
    15181 cgaccgcgac ctatctagcg acactagccc gtcgggcatg acgaacccgc ggaccaccgg
    15241 tgtgctcgtc accacgtacc ggatgttgag cgccttgacc gcctcggcca cccgcttgtc
    15301 ggtgtcggcg tcgtcggcgt aggcccagaa gatgaaccgg tggtaacccg gaccctgctg
    15361 caccgggtag tcgtagtgag tccacagcgg atgtagcccg gcggcggcgt acatccacgc
    15421 tgtgccgtcg gtgttcgcgt cgccgatcag ggtgtcgcgc gcccccggca acgtggccag
    15481 atacgcccac gcctccaggt ccttggagtc gacgatcacc ctgtcgtact tctcgccgaa
    15541 cagaaactgg gcccgcggta gatacgcgag cccgatgccg atactgacgg ccaccacgat
    15601 ggcggccgta gccgcgttcc aggcccgtgg actgcccctt ccgaccagcc ggcgcccgcc
    15661 tgcgacgaca accgacgcca gcgtgaacac gccgatgccg acggccgcgg tgagcagcat
    15721 cgtgaccacc gccgacagtc gccgcgggtc gctgtagaac aagtcgctga acttgccggt
    15781 gagctccccg atggggccgc cgaacggcgc cgacgaatgc acgatcgaca ccaccagcag
    15841 gaaccaggcc gccagcggcc accagacccg tcggtaaatc aggacgaccc cgccgacggc
    15901 ggccaagatg atgagcaccc actggatcgg gaagtcgttg agatgccggg tgtgctgcac
    15961 gactgcgtcg atcagtccgc gcttcttgcc ctcgtgcgtg acgaacgcgt gcccgacgat
    16021 gatctcggcc tgccgcagca cgcccaaaaa ctgcggcagc agcagtgcca ccgtcggaat
    16081 cgcaatgagc agcagattga cgaagtcggc gacgcggccc cggacgggat gccacagcgc
    16141 gtcgaacaac caccacgcag ccacgaacag aacggtcacc acgccaccgg tgatgtgcac
    16201 cgagaacacg ccgagcagcg ccagcaccgc aagcggaata cggtcgcggt gccgcagcgc
    16261 ggacaccacc agcaccatgg tcgggacggc gacgccgtag gcggcgaggt tgggcatcga
    16321 ggccgtgtcg aactcgatgt aggggagcgc ggtgaacgaa cccgacagtg cggctgcggc
    16381 ggcggcggcg ccggccgtac gccactcgtc ggttcgggtg cgcagcaggt gccacgtcag
    16441 agtcgcggcg ctgaccggga acagccagac cgaagcggcc actgagttca gcgtgtacgc
    16501 agtggtcggt gcggcgccgg tgagttggct gaacaccgct gccacagcgt gaaacgtcga
    16561 cgggtagtac agcgcttcac gggtctcgac gttgcgcagc tcgcccatgt gggtcggcga
    16621 ggcctgtccg gtgtcgagga tgaagcgaat cgtgttggcg tgccagaccg aatcccagtt
    16681 actcggaatg gattgccaat tcggcatccc gcgaatcgcc gccaacccga tcagcaaggc
    16741 gcccaacagg acacctgccg ccaacactag cgcgggaccg cgggctaccg cgcgggtctc
    16801 tgcgtcgacg tcgcgaacac gggcgagcgc gatccgcaaa caccacgcgg cagctgtcgc
    16861 gacggccaga gcaaacaatg cggtccatcc gttccacggg acaccaagtg cgccaaacgg
    16921 aacgatggcc aacgccacga cgccgtaggt cagcgccgga ccaaccgcaa ccgcggcggg
    16981 ccaagttagc cgaccgacac gtgcgaagat tgccccgggg atcaccagca aaaacagtgc
    17041 gattagcact ccgaacccca agctcactgg actagtatgg ctgcccaggt gacccggtcc
    17101 gggcacggca tgggcagcga cagggcacgg ccgggcatcg gccaagatcg tgtcggtttg
    17161 tgaaaatcac agacgctcta aggtgggctc gcatggcata cgacgtcgcc cgggtgcgtg
    17221 gtctgcatcc gtctttgggc gatgggtggg tgcacttcga cgcgcagaac ggcatgctgc
    17281 tgcccgattc cgtgggcaga gcggtatcga ccgctttccg cggatcgatg ccgaccccga
    17341 taggacccca tccgtcggcg caacgcagcg ccgcggtgct cgaagcagcg cgccaggcaa
    17401 tcgccgatct ggtcaatgcg gacccgcggg gtgtggtgct cggcgccgac cgcgcggtct
    17461 tgctgacgtc tctggccgac gcgtcgtcgt cgcgggtcgg cctcggctac gaggtggtgg
    17521 tgacccggct cgatgacgag gcgaacatcg cgccgtggct gcgcgcggcg aatcgctatg
    17581 gcgccaaggt gaagtgggcc gaggtcgaca tcgagaccgg tgaactgccg acctggcagt
    17641 gggagggtct gatcacgcgg tcgacgcggt tggccgcaat cgcctctgcc tcttcgacgt
    17701 tgggcgcggt caccgacctg cgggcggtga cgaagctcgt gcacgagggc ggcggattgg
    17761 tggtcgtcga tcactccgcc ggcgcgccgt accggctgat cgacatcgac gaaatcgatg
    17821 ccgacgtggt cgcgttgaat gcggtggcat ggggtggtcc gccgatcggc gcgctggtct
    17881 tccgtgaccc gtcaatgatc aactcgttca gctcggtctc gctgaacccg tatgcgaccg
    17941 gccccgcgcg actcgaggtg ggcacacacc aattcggcta cctcgcaggc gtcgtcgcca
    18001 gcatcgagta tttggctggg ctcgacgagt ctgcgcaggg gtcgcggcgc gaacgccttt
    18061 cggtgtcaat gcaatcggcg tcgacgtacc tgaaccgggt gttcgactac ctgctggtct
    18121 cgctgcgctc cctgtccgcg gtgatggtga tcggcaggcc ggatgtgcat attccagtgc
    18181 tgagtttcgc ggtcagcgac gtgccggccg agcgggtggt gcagcggctg gccgacaacg
    18241 gcgtcctggc gatctccaat gtcagctcgc gtgtgctgga tgtcctcgga gtcaatgacg
    18301 tgggtggcgc ggtgaccgtt gggctggcgc actatacgac gaccgcggag gtcgaccagt
    18361 tggtgcgcgc cttggcctcg ctcggctagt cggccacgcg cagaagaatc ttcccggata
    18421 cctcgcccga gttcagcagc tcgtgtgctg ccccggcctg ttcgatcggg aattcggcgc
    18481 cgacgatcgg gcgcacttga ccgtcagcga tcatcggcca caagttggcg accacttcgg
    18541 cgacgatgtc gctcttgctg ccggcaccac tgaccggacg cgaccgcagc gcggtggcga
    18601 tcacgccggc gcgcttgccg agcagcttgc cgatgttgag ttcggccttc acgccgccct
    18661 gcagcccgat gatgatcaac cggcccccgt cggcgagagc gtcgacattg cggtcgaggt
    18721 atttggctcc catgatgtcg aggatcacgt cggcgccgcc tgcgctgcgt actcgctcca
    18781 cgaagtcctc gtcgcggtaa ttgatggtga tttcggcgcc cagctcgctg cacaggtcga
    18841 gtttgttctg cgccccagcg gttacggcga ccctggcgcc gagcgcgcgt gccacctgga
    18901 tgccgtgcgt gccaatgccg ctggcgccgc cgtggatcag gagaagtcga ccggccgaca
    18961 ggtgggcggt catcatcaga ttcgaccaga ccgtgcacgc cacttccggc agtgcggcgg
    19021 cgtgatgcag cggcacatcg gcgggaatcg gcatcacctg gccggcgggg actgcgacga
    19081 attccgcgta acccccgcct gccagcaaag cacagacttg ttgcccgacg acccaatcga
    19141 cgacttcgtc accgaccttg gcgatcgttc ccgacacctc gagcccgagg atctggctgg
    19201 ccccgggagg gggcggatag ttgcccgcgg cttgcagtag gtcggcgcgg ttgacacccg
    19261 ccgcacttac cttgatgagg acttcgctgt cgccgggttc aagatcgggg atttcttgcc
    19321 aggtgagctg atcggctgct tcggccacga tcgcgcgcat tggggtaacc ctactatgcg
    19381 ttgaaagcag tctggatttc tcacccggag caatgcttgc ctgctctact atagcgaaat
    19441 ggaggggatc attgccgggc gtactgccag tgacatgcct ggtttagata tcgctgagca
    19501 gaggtcgtgg caaaacttcc tcgactcagc gttgaggatg tacgcgaccc ttaaccggtc
    19561 gctggtggac gcacatcatc tgacgctgaa tgacgtgcgg ctgctcgaca ttttggacaa
    19621 gtccgcgacc ggctcggcgc gcatggggga tctcgccgac gggctgatgt cattgccgag
    19681 ccgggtgacc cggcagatcc ggcgcttgga attgcagggc ctggtgcgcc gcggtgccag
    19741 ccctgatgac ggccggggcg tgctggcctc aatcaccgac gaagggcgtg tcgcggtgac
    19801 cgaggcgatg ctgacgtatg gccagggggt tcgcgcgcat ttcctcggca gattgtctcg
    19861 accgcagatc gccgcgatgg gcgagaactg ccgtcgcatc agcgtcgggc tcaagggaac
    19921 cccgtcggcc gcgaaggtcg gcagggtcta gaccccttag gcttacacgc ggtggcgtgg
    19981 cagagcggcc taatgcactc gccttgaaag cgagagacgg ctaacaccgt ccgggggttc
    20041 aaatccctcc gccaccgcag gctattcgcc ggtgaactgc ggcggccgct tcgcgaacgt
    20101 cgcggtgatg ccctcggtca ggtcctt
//
LOCUS       DASRJA010000163         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3262728,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000163 DASRJA010000000
VERSION     DASRJA010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3262728"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..429)
                     /locus_tag="VFC01_07790"
     CDS             complement(25..429)
                     /locus_tag="VFC01_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79573.1"
                     /translation="MRTTASITAILVGIIMAIAGVVTWVVVSTTLADQKITVADDANC
                     AAGDDVNGPISAYCQADIIDHHTKAITGGKTYAELAQDDPKRATAMDSAFLQASLFTS
                     VVAFGVAAMAVVLGIVLTLIGFALRAPVRATS"
     gene            595..2040
                     /locus_tag="VFC01_07795"
     CDS             595..2040
                     /locus_tag="VFC01_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009477910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZN79574.1"
                     /translation="MDVLDIARWQFGIITVYHFLFVPITIGLSAVIAGMETAWVRTGK
                     QEYLRLTKFFGKLFLINFAIGVVTGIVQEFQFGMNWSDYSRFVGDIFGAPLAVEGLLA
                     FFLESTFLGLWIFGWDKLPRGLHATTMWLVHLGTLFSSWFILAANSWMQHPVGYRFNP
                     RSGRAELIDFWAVMFNKVQLVTFPHVVLAAYMTAGAFVAGVAAWLYARKEHVADQPLY
                     RKAIRIGAIVALVAGLGVAITGDAQGKIMTEVQPMKMAAAEALYESEQPASFSIFTVG
                     TPDGRSEKFAIKIPNLLSFLATGTWDGKVEGINDLRATYERQFGQDPGAAYYSPGDYV
                     PYIPVTYWTFRFMIGVGLLAAAGAALILWLTRKGRTPTSRWFGLMALSIPVLAVFGSS
                     FGWIFTEMGRQPWAVFGLMTTANGVSPSVSTFEAATSVIVLTVLYAVLAVIELGLLAK
                     YIRKGAEPFVEPPDPSLRGPQDDDQPLTFAY"
     gene            2051..>2492
                     /gene="cydB"
                     /locus_tag="VFC01_07800"
     CDS             2051..>2492
                     /gene="cydB"
                     /locus_tag="VFC01_07800"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005254814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZN79575.1"
                     /translation="MELTTVWFILIAVLWIGYFTLEGFDFGVGMLLPVLARDDTERRV
                     MINTIGPVWDGNEVWVLVAGGATFAAFPEWYATLFSGFYLPLLLILVALIVRGLAFEY
                     RHKRAEAQWTAGWDRALVVGSFVPAVLWGVAFANIVHGVPIDADK"
BASE COUNT          383 a          834 c          831 g          444 t
ORIGIN      
        1 gtgggcgttg atgaaggtcg cggcctacga ggtcgcgcgg acgggtgcgc gcagcgcgaa
       61 cccgatcaac gtcaggacga tcccgaggac gaccgccatg gccgcgaccc cgaaggccac
      121 gaccgaggtg aacagggagg cctgcaggaa cgcggagtcc atcgcggtcg cacgcttcgg
      181 gtcgtcctgg gcgagctcgg cgtaggtctt tccgcccgtg atcgccttgg tgtgatggtc
      241 gatgatgtcc gcctggcagt acgccgagat cgggccgttc acgtcgtcgc ccgcggcaca
      301 gttggcgtca tcggcaaccg tgatcttctg gtcggccagc gtggtggaga cgaccaccca
      361 cgtgaccacc cctgcgatcg ccatgatgat gccgacgagg atggccgtga tcgatgcggt
      421 cgtgcgcatg tccgttctcc catctgcgct ttcaagagca acatcgagcc gctgaacgca
      481 ggtccactgc acatcggtgg cagatccgcc ggatcaccca caacggatga ggtgcagccg
      541 ctccgcgggg tgcaacgtcg tcggccaggc cggcgaccac gcgcaggagg ccctgtggac
      601 gttctcgaca tcgcacgctg gcagttcggc atcatcaccg tctaccactt cctgttcgtc
      661 ccgatcacga tcggcctgtc cgcggtcatc gccggcatgg agaccgcctg ggtgcgcacc
      721 ggtaagcagg agtacctccg cctgacgaag ttcttcggga agctgttcct gatcaacttc
      781 gcgatcggcg tcgtgaccgg catcgtgcag gagttccagt tcggcatgaa ctggtccgac
      841 tactcgcggt tcgtcggcga catcttcggt gcgccgctcg ccgtcgaagg actgttggca
      901 ttcttcctgg agtcgacgtt cctcggactc tggatcttcg ggtgggacaa gctgccgcgc
      961 ggcctccacg ccaccaccat gtggctcgtg cacctcggca cgctcttctc gtcgtggttc
     1021 atcctcgcgg cgaactcctg gatgcagcac ccggtcggct accggttcaa cccccgcagc
     1081 ggcagggccg agctgatcga cttctgggcg gtgatgttca acaaggtgca gctcgtgacc
     1141 ttcccgcacg tcgtcctggc ggcgtacatg accgcgggcg cgttcgtggc cggagtcgcc
     1201 gcctggctct acgcgcgcaa ggagcacgtc gccgaccagc cgctctaccg caaggcgatc
     1261 cgcatcggtg cgatcgtcgc gctcgtcgcc ggcctcggcg tcgcgatcac aggtgacgcg
     1321 cagggcaaga tcatgaccga ggtgcagccg atgaagatgg cggccgcaga agcgctgtac
     1381 gagagcgaac agcccgcgtc cttctccatc ttcaccgtcg gcaccccgga cggcaggagc
     1441 gagaagttcg cgatcaagat cccgaacctg ctgtcgttcc tcgccaccgg cacctgggac
     1501 ggcaaggtcg agggcatcaa cgacctgagg gcgacgtacg agcggcagtt cgggcaggac
     1561 ccgggcgcgg cgtactacag ccccggcgac tacgtgccgt acatcccggt gacctactgg
     1621 acgttccgat tcatgatcgg tgtcggcctc ctggccgccg caggcgccgc gctgatcctc
     1681 tggctgaccc gcaagggacg cacgccgacc agccgctggt tcgggctgat ggcgctgagc
     1741 atcccggtgc tggccgtctt cggcagctcg ttcggctgga tcttcaccga gatgggacgc
     1801 cagccctggg ccgtgttcgg cctgatgacc accgcgaacg gcgtctcgcc cagcgtcagc
     1861 acgttcgagg ccgccacgtc cgtcatcgtg ctcacggtgc tgtacgccgt gctggcggtc
     1921 atcgagctcg gcctgctggc gaagtacatc cgcaagggcg cggagccttt tgtcgaaccg
     1981 ccggacccca gcctgcgggg accgcaggac gacgaccagc cgctgacgtt cgcctactga
     2041 ggagaccgcg atggagctca ccaccgtctg gttcatcctg atcgcggtcc tctggatcgg
     2101 ctacttcacc ctcgaagggt tcgacttcgg ggtcggcatg ctgctgccgg tgctggcgcg
     2161 cgacgacacc gagcgccggg tcatgatcaa cacgatcggc cccgtctggg acggcaacga
     2221 ggtgtgggtg ctggtcgccg gcggggccac gttcgcggcg ttccccgagt ggtacgccac
     2281 tctcttcagc ggtttctacc tgccgctgct cctgatcctg gtcgcactca tcgtgcgcgg
     2341 tctggcgttc gagtaccggc acaagcgcgc agaggcgcag tggactgccg ggtgggaccg
     2401 ggccctggtc gtcggatcgt tcgtgccggc ggtgctgtgg ggcgtcgcgt tcgcgaacat
     2461 cgtgcacggc gtgccgatcg acgccgacaa gg
//
LOCUS       DASRJA010000164         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_246957,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000164 DASRJA010000000
VERSION     DASRJA010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246957"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFC01_07805"
     CDS             <1..318
                     /locus_tag="VFC01_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293297.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase"
                     /protein_id="HZN79576.1"
                     /translation="TVDGRHHYPQDIEATTAAASSMVRRGYVTAFSTDDGLVIIAERA
                     SGTRRADPAPAVEAIRAAVSNVHGLTVSDIRFVPAGAIPRTTSGKLARHASRTEYLRG
                     ELG"
     gene            424..1911
                     /locus_tag="VFC01_07810"
     CDS             424..1911
                     /locus_tag="VFC01_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HZN79577.1"
                     /translation="MHEDFDRCYRAVQSKDARFDGWFVTAVLTTKIYCRPSCPVRPPF
                     ARNMRFYPTAAAAQQAGFRACKRCRPDASPGSPEWNVRGDVVARAMRLITDGTVDREG
                     VTGLAARVGYTTRQLERLLQAEVGANPLALARAQRSQTARVLIETTELPFGDVAFAAG
                     FSSIRQFNDTVRTVCDLTPTALRQRARARFGRGGPAATGALSLRLPVRTPFAYEGLFG
                     HLAASAVPGVEEVRDGAYRRTLRLPNGNGIVSLTPQPDHVQCQLVLDDFRDLSSAIAR
                     CRRLLDLDADPEAVVDLLSADPDLTALVAKAPGQRIPRTVDEQELAVRVVLGQQVSLK
                     AARTHARRIVTAYGQPVADANGGLTHVFPAVEHLAEIDPAHLAFPKSRQRSLTTLITA
                     MANGDVVLDAGCEWNSAREQLLALPGIGPWTADVIAMRGLGDPDAFPVSDLGVRLAAT
                     QLGLPGDARPLTERSGRWRPWRSYATQHLWTALDHAVNEWPPKEK"
     gene            1911..2408
                     /locus_tag="VFC01_07815"
     CDS             1911..2408
                     /locus_tag="VFC01_07815"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZN79578.1"
                     /translation="MVGLQFRSMDSPVGLLTLAGKDGRLMHLRMVDQTYEPSHDGWEA
                     DDEAFPEAVEQLDAYFAGDLIEFDLDLDLVGTGFQRRVWEALLTIPYGETRSYGEIAR
                     QIGSPGAFRAVGLANGHNPIGIIVPCHRVIGSNGSLTGYGGGLDRKRALLELEKSRIA
                     PALFD"
     gene            complement(2471..2585)
                     /gene="rrf"
                     /locus_tag="VFC01_07820"
     rRNA            complement(2471..2585)
                     /gene="rrf"
                     /locus_tag="VFC01_07820"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(2695..>2938)
                     /locus_tag="VFC01_07825"
     rRNA            complement(2695..>2938)
                     /locus_tag="VFC01_07825"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          469 a          906 c          996 g          567 t
ORIGIN      
        1 acagtggacg gccgtcatca ctacccgcag gacatcgagg ccaccacagc ggctgcctcg
       61 tcgatggtga ggcgcggata tgtcacggca ttctccacgg acgacggtct ggtcatcatc
      121 gccgaacggg cttcgggaac ccgccgcgcc gacccggccc cggcggtcga agccatccgc
      181 gcggcagtgt cgaacgtcca cggcctcacc gtctccgaca tccgcttcgt acctgccggg
      241 gcgatcccgc gcacgacgag cggaaagctc gctcgacatg cgtcgcgcac cgagtacctt
      301 cgcggcgagc tcggctgagg ccccggccta cagtcccgaa tgcgactgcg tccgccgcgc
      361 accaccaacc acgatgtcgt ttttccgcca gtgatgtcgg tgggctcgtg taattgtcga
      421 tgcatgcacg aagatttcga ccgctgctac cgggccgtcc agtccaagga cgcccggttc
      481 gacggctggt tcgtcaccgc cgtgctgacc acgaagatct actgcaggcc cagctgcccg
      541 gtccgcccgc cgttcgcccg caacatgcgg ttctatccga cggccgcggc cgcacaacag
      601 gccggcttcc gggcctgcaa gcggtgccgg cccgacgcat cgccgggttc accggagtgg
      661 aatgtgcgcg gcgatgtcgt agccagggcg atgcggctga tcaccgacgg caccgttgac
      721 cgggagggtg tgaccggtct ggcggcgcgc gtcggctaca ccacccgcca gctcgagcgg
      781 ctgctgcagg ctgaggtcgg tgccaatccc ctggcgttgg ccagggcgca gcgcagccag
      841 accgcccgcg tattgataga gacgacggag ttgccgttcg gcgacgtcgc gttcgccgcg
      901 ggcttctcga gcatccgcca gttcaacgac acggtccgca cggtgtgcga cctgacgccg
      961 acggccctgc gccagcgtgc tcgtgcccgg ttcggacgtg gcggacccgc cgccacaggt
     1021 gccttgtcgc tgcggctgcc ggtgcggacg ccgtttgcgt acgagggatt gttcggccat
     1081 ctcgcggcga gtgccgtgcc aggcgtcgag gaagtgcgcg acggcgcgta tcgacggacg
     1141 cttcggctac ccaacggcaa cggaatcgtc agtctcacac cgcaacccga ccacgtgcag
     1201 tgccagttgg tgctggacga tttccgtgat ctgtccagtg cgatcgcgcg gtgccggcgc
     1261 ctgctcgacc tcgacgcgga ccccgaggct gtcgtcgatc tgttgagcgc cgatccggat
     1321 ctgaccgcgc tagtggccaa ggcgcccggg caacggattc cgcggacggt cgacgagcag
     1381 gagcttgccg tgcgggtggt gctcggccag caagtatcgt tgaaggccgc gcgcactcat
     1441 gcccggcgca tcgtgaccgc ctacggccag ccggtagccg acgccaatgg cggtctgaca
     1501 catgtctttc cggcggtcga gcacctcgcc gagatcgatc cggcgcatct ggcattcccg
     1561 aagtcgcgcc agcgctcgct gaccacgctg atcaccgcga tggcgaacgg cgatgtggtg
     1621 ctggacgccg gctgcgagtg gaacagtgca cgcgagcagc tgcttgcgct gccgggcatc
     1681 ggcccgtgga ccgccgacgt gatagcgatg cgcggcctcg gtgaccccga cgcgttcccg
     1741 gtgagcgatc tcggtgttcg gttggcggcc acgcagctgg gactgcccgg cgacgctcgg
     1801 ccattgaccg agcgcagtgg acggtggcgg ccctggcggt catatgcgac gcagcacttg
     1861 tggacggctc ttgatcatgc ggtcaacgaa tggccgccga aggagaagtg atggtgggac
     1921 tgcaatttcg gtcgatggat agccccgtcg ggctgttgac gctggcaggc aaggacgggc
     1981 gattgatgca tctccggatg gtcgaccaga cctacgagcc gagccatgat gggtgggaag
     2041 ccgacgacga ggcgttcccc gaagccgtcg aacagctcga tgcgtacttc gccggcgatt
     2101 tgattgagtt cgatcttgat cttgatctgg tcggcactgg attccagcgg cgggtctggg
     2161 aggcgctgtt gacgattccc tacggcgaga ctcggtcgta cggcgagatc gcccggcaga
     2221 tcgggtcacc gggggcgttt cgcgccgtcg gattggcgaa cggccacaat ccaattggca
     2281 tcatcgtccc atgccatcgc gtgatcggat cgaatggcag ccttactgga tatggcggcg
     2341 ggctcgatcg gaaaagggcg ttgctcgaat tagagaaaag ccgcatcgcg cccgcactat
     2401 tcgattgaac aatgcaataa aaatgcctct gccctccaat cgaaattgga gggcagagga
     2461 cttaaattgt gttcggcggt gtcctacttt tccacccggg ttgggtagta tcatcggcgc
     2521 tggcaggctt agcttccggg ttcgggatgg gaccgggcgt ttccctgccg ctatggaccg
     2581 ccgtaacttt attcacttgt cgaagtgccc cagtgtgggt ggggaggttt gtgttattgg
     2641 tggtggggtg cagtggatga ctgctgtgga tgttgtgtgt ggttgcgagg ttttgtgggt
     2701 aagttttcgg ccggttagtg ccagttccct gtcgtattac tacggttata ggtctggtct
     2761 attgatcccg tggtctgcgg ggggccttat cccactgaat gggtgagaag cctggtcttg
     2821 gagggggttt cccgcttaga tgctttcagc ggttatcctg tccgaacgtg gctatccagc
     2881 ggtgcccctg gtgggacaac tggtggacca gaggttcgtc cgtcccggtc ctctcgta
//
LOCUS       DASRJA010000165         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1570759,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000165 DASRJA010000000
VERSION     DASRJA010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570759"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="VFC01_07830"
     CDS             <1..157
                     /locus_tag="VFC01_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-(1-2)-phosphatidylinositol
                     mannosyltransferase"
                     /protein_id="HZN79579.1"
                     /translation="TADNDPDAIARAVSSIISRPERLRRNSARRRAEQFTWPRAAAGM
                     LNALGAG"
     gene            179..850
                     /locus_tag="VFC01_07835"
     CDS             179..850
                     /locus_tag="VFC01_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HZN79580.1"
                     /translation="MAEDTERARSLWFALLLTLANGFLDAHTYIARGGVFANVQTANV
                     IFGAIDTSKREWVLALAHLWPLLAFIAGVGLASFIKAGRVERIVPKPLRWTMAVQAVA
                     LAIIGFVPASVPHSYVTVPISFLAAMQIGLFRNIGELVYLPVATTGNLMRFVEAGYDG
                     FVEKHAESRRAFGVYGSLILAFAVGALVGAIASRAWGVHAIWLPAGFLAITLCLFIID
                     ERHLR"
     gene            865..1263
                     /locus_tag="VFC01_07840"
     CDS             865..1263
                     /locus_tag="VFC01_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79581.1"
                     /translation="MIRSPIVLTAAVAVALAGTAAVARAEPSPPPCAYTLSPPQVVQL
                     SGTNVVTATLSPAACDRSVAYLTVACVQLQGSDGPGQCAQQNGLLTAQVFYQPYRPGA
                     TYVATGRGCATTGNPPQPVCQPMGPLTATL"
     gene            complement(1268..1798)
                     /locus_tag="VFC01_07845"
     CDS             complement(1268..1798)
                     /locus_tag="VFC01_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZN79582.1"
                     /translation="MYQGAARPARPPADTVRVVLVLDVPAGSAELPSRTAQLADEFGR
                     LIAQSVPGVRARKAVVSAGPSMKAAADWSTGLTIDRFHREVRIDGERVRLTYREFELL
                     CYLAARPRRPVSRAELMREVWHDRAPGGDVSLRTVDTHVRRLRAKLGPHARVLTTIRG
                     RGYRFDPGPDVRYLTA"
     gene            complement(1859..>2531)
                     /locus_tag="VFC01_07850"
     CDS             complement(1859..>2531)
                     /locus_tag="VFC01_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HZN79583.1"
                     /translation="ILDFVREALAVCDIDLQIVIFESFDEPNDLLIARRLHANFFQYL
                     PFLDDFNRRTGNRLVPVAPVHIEPFGLYSSSIPDLDAIPDYAEVALPSDPVNVDRSLV
                     MLNQLGLIECAPARRGLVSVGDVRSNPRRLVFKELTSWLLGDVREDFDLVFLFGNQAM
                     EWGVDTGSALYCDRGNPAYAEYLVARADNCESIGVRALAEALNCESTREFITSTYAGQ
                     VLAAF"
BASE COUNT          422 a          897 c          808 g          404 t
ORIGIN      
        1 caccgccgac aacgacccgg acgccatcgc ccgcgctgtc tcgtcgatca tcagcaggcc
       61 ggaacggctg cgccgcaaca gcgcccgccg gcgcgccgag cagttcacct ggccgcgcgc
      121 cgccgcgggc atgctcaacg cgctgggcgc aggctagccg ccgcgctacc ttggccgggt
      181 ggccgaggac accgaacgcg cgagaagcct gtggttcgcc cttctgctca ccctggccaa
      241 cggtttcctc gacgcccaca cctacatcgc ccgcggcggg gtcttcgcca acgtgcagac
      301 cgccaacgtg atcttcggcg ccatcgacac ctcgaaacgg gagtgggtgt tggcgctggc
      361 gcacctgtgg ccgcttcttg ccttcatcgc gggggtcgga ctggcgtcat tcatcaaggc
      421 tgggcgcgtc gagcgcatcg tgccgaaacc gctgcgatgg acgatggctg tccaggccgt
      481 cgcgctcgcg atcatcggct tcgtgccggc atcagtgccg cacagctatg tgacggtgcc
      541 gatctcgttt ctggcggcaa tgcagatcgg gctgttccgc aacatcggcg agctcgtcta
      601 cctacccgtc gccaccaccg gtaacttgat gcggttcgtc gaggcgggat acgacgggtt
      661 cgtcgagaag catgcggagt cacgccgggc gttcggcgtg tacggctcgc tgatcctcgc
      721 gttcgctgtc ggcgcgctgg tcggcgctat cgccagccgg gcgtggggag tacacgcgat
      781 ctggctgccc gccggattcc tcgcgatcac cctgtgcctg ttcatcatcg atgaacggca
      841 cctgcgatga actaccatcc aggcatgatc cggtcgccga tcgtcctcac cgcagcggtg
      901 gcggtggccc tcgccggaac ggctgccgtg gctcgcgccg aaccaagccc accgccgtgc
      961 gcatacacac tgtcgccgcc ccaggtcgtg cagctttccg gcaccaacgt ggtcacggcg
     1021 acgctgtcgc cagccgcgtg tgaccgttcc gtcgcgtatc tgaccgtcgc gtgcgtgcag
     1081 ctgcagggca gtgacggtcc gggccaatgc gcgcagcaga atggccttct gacggcgcag
     1141 gtgttctacc agccgtatcg gcccggagca acgtacgtcg ccaccggcag gggatgcgct
     1201 acgaccggca acccgccgca gcccgtctgc cagccgatgg gcccgctgac cgcgacactg
     1261 taaacgatta ggccgtgagg taccgcacgt cggggcccgg gtcaaagcgg tagccccgac
     1321 cacggatcgt ggtcagcacc ctcgcgtgcg ggccgagctt ggcgcgtagc cgccggacat
     1381 gcgtatccac cgtccgaagc gacacgtcgc cgcccggcgc ccggtcgtgc cacacctcgc
     1441 gcatcagttc ggcgcgtgac accgggcgcc gcggccgcgc cgccaggtag cacagcagct
     1501 cgaactcgcg gtaggtgagc cgcacccgct caccgtcgat gcgcacctcg cggtgaaacc
     1561 ggtcgatcgt caaaccggtt gaccagtccg cggccgcctt cattgacggg ccagctgata
     1621 ccaccgcctt gcgcgcccgc acccctggca ccgactgcgc gatcagccta ccgaactcgt
     1681 cagccaattg cgctgtgcga cttggtaatt ccgccgaacc agcggggacg tccaggacaa
     1741 ggaccacccg gacggtgtca gcaggtggcc tcgcgggccg cgctgcgcct tggtacaccc
     1801 gttcactgtc gcgacccggc aacggcccga acagggttta ggtcggcctg agcgcaactc
     1861 agaacgccgc cagcacctgc ccggcgtagg tgctcgtgat gaactcgcgg gtggactcgc
     1921 agttcaacgc ctctgcgagc gcccgaacac cgatgctctc gcaattgtct gcccgcgcca
     1981 ccaggtactc cgcgtacgcc ggattgccgc gatcgcagta cagcgcgctg ccggtgtcga
     2041 cgccccactc catcgcctgg ttgccgaaca ggaacaccaa gtcgaaatcc tcgcgcacgt
     2101 cgccgagcag ccagctggtc agctccttga acaccagccg gcgcggattc gaccgcacat
     2161 cgccgacgct gaccaggcct ctgcgcgcgg gcgcgcattc gatcagcccc agctggttga
     2221 gcatcaccaa cgaccggtcc acgttcactg gatccgacgg cagcgcgacc tcggcatagt
     2281 cgggaatcgc gtcgagatcg ggtatcgacg aggagtacag cccgaacggc tcgatgtgca
     2341 ccggcgccac cggcaccagc ctgttgccgg tgcgacggtt gaaatcgtcg aggaacggca
     2401 ggtactgaaa gaagttcgca tgcaaccgac gggcgataag caggtcgttg ggctcgtcga
     2461 agctttcgaa gatgacgatc tgcaggtcga tgtcgcagac ggccagcgct tcacgcacga
     2521 agtcgaggat c
//
LOCUS       DASRJA010000166         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1009899,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000166 DASRJA010000000
VERSION     DASRJA010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009899"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VFC01_07855"
     CDS             <1..698
                     /locus_tag="VFC01_07855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN79584.1"
                     /translation="PILEATETMQVATGIVNIWSADANEVASSYHRIEKAFPGRFLLG
                     IGVGHPENTQEYRKPYDTLVEYLDVLDAKKVPTSRRVVAALGKKVHELAAKRSAGSHP
                     YLTTPEHTAQAREWIGPTVYLAPEHKVVLTTDAEEARAIGRKTVDFYLNLSNYLNNWK
                     RLGFTDEDIAKPGSDKLIDAVVAHGSGDDVAKRLNEHLDSGADSVLIQVLGGKDKLLP
                     TLTELARPLGLKG"
     gene            726..1721
                     /gene="rfbB"
                     /locus_tag="VFC01_07860"
     CDS             726..1721
                     /gene="rfbB"
                     /locus_tag="VFC01_07860"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HZN79585.1"
                     /translation="MRLLVTGGAGFIGANFVHGIVREHPDDRVTVLDVLTYAGSRESL
                     APVADDIRLVQGDITDAELVSKLIAEADAVVHFAAETHVDNALANPEPFLHANVVGTF
                     TVLEAVRAHGVRLHHVSTDEVYGDLELGSAQRFTESTPYNPSSPYSSTKAAADLLVRA
                     WVRSYGVAATISNCSNNYGPYQHVEKFIPRQITNVLSGRRPKLYGNGANVRDWIHVDD
                     HNSAVWRIIADGQVGRTYLIGAEGERDNLSVMRTILRLMGRDPDDFDHVTDRAGHDLR
                     YAIDPSPLYHELGWGPKHTDFEEGLRATIEWYRDNESWWRPLKDAVEAGYEERGQ"
     gene            1718..2326
                     /gene="rfbC"
                     /locus_tag="VFC01_07865"
     CDS             1718..2326
                     /gene="rfbC"
                     /locus_tag="VFC01_07865"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HZN79586.1"
                     /translation="MKARELSVPGAWEITPQVHTDSRGLFFEWFTDSGFTEFAGHRLD
                     LRQANCSVSSAGALRGVHFAQLPPSQAKYVTCLTGSVFDVIVDIRVGSPTFGKWDAVV
                     LDDQHRRSVYLSEGLGHAFLALEDDSTVMYLCSAEYDPGREHTINALDPALDIAWPAG
                     VDQPILSDRDRDAPSLAQAQSAGLLPTWEETQAFVAKLRSHI"
     gene            2350..>2917
                     /locus_tag="VFC01_07870"
     CDS             2350..>2917
                     /locus_tag="VFC01_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN79587.1"
                     /translation="MNRIDVQFDVAGDACAAWKYEPETTDSDDAPIVVMAHGLSGTRR
                     DRLGAFAERFAQRNCFALVFDHRGFGDSGGTPDLFEPGRQLEDWRHAIAFARRRAGVD
                     AKRVVTFGSSMGGGNALAAAVHDGEVAAVISQVPFLDIRQAYRAKPRVRARMMLAASR
                     GEYMPAVGQPDEAAFINAPESEAGWRHVV"
BASE COUNT          538 a          935 c          960 g          484 t
ORIGIN      
        1 agccgatcct cgaggccacg gagacgatgc aggtcgccac cggcatcgtc aacatctggt
       61 cggcagacgc gaacgaagtc gcgtcctcgt atcaccgcat cgaaaaggcc tttcccggac
      121 gcttcctgct cggcatcgga gtcggccacc ccgagaacac acaggagtac cgcaagccct
      181 acgacacgct cgtcgagtac ctcgacgtgc tcgatgccaa gaaggtgccg accagtcgtc
      241 gggtcgtggc cgcgctgggc aagaaggtgc acgagctggc ggcaaagcgc agtgcgggtt
      301 cgcacccgta cctgacgacg ccggagcaca cggcgcaggc acgcgaatgg atcgggccga
      361 cggtgtattt ggcgcccgag cacaaggtgg tgctgaccac agacgccgag gaagcccgcg
      421 cgatcggccg caagaccgtc gacttctatc tgaacctgag caattacctc aacaactgga
      481 agcggctcgg cttcacggac gaggacatcg cgaagcccgg cagcgacaag ctgatcgacg
      541 cggtggtcgc gcacgggagc ggtgacgacg tcgccaagcg gctcaacgag catctggact
      601 cgggtgccga ttcggtattg atccaggtgc ttggcggcaa ggacaagctg ttgccgacgc
      661 tgaccgaatt ggcgcgtccg cttggcctga agggctgacc ggaccacgac cgctacggtg
      721 ggttcatgcg gttgctggtc accggcggag cggggttcat cggcgctaac ttcgtgcacg
      781 gcatcgtgcg cgagcacccc gacgaccgcg tgacggtgct cgatgtgctg acctatgcgg
      841 gcagccgcga atcactggcc ccggtcgcag atgacatccg gctggtgcag ggtgacatca
      901 ccgatgccga gttggtgtcc aagctcatcg ccgaggccga cgccgtcgtg catttcgccg
      961 ccgagaccca cgtcgacaac gccctggcca atccagagcc gttcctgcac gccaacgtcg
     1021 tcggcacctt cacggtgttg gaggcggtgc gcgcacacgg tgtgcggttg catcacgtgt
     1081 cgaccgacga ggtgtacggc gacttggaac tcggttctgc gcaacggttc accgagtcga
     1141 cgccgtacaa cccatcgagc ccgtactcgt cgaccaaggc agcggccgac ttgctcgtac
     1201 gcgcatgggt gcggtcctac ggggtggccg caacgatatc gaactgctca aacaactacg
     1261 ggccgtatca acacgtggag aagttcatcc cccgccagat caccaatgtg ctgtccgggc
     1321 ggcggccgaa gctctacggc aacggcgcga acgttcgtga ctggatccat gtcgacgacc
     1381 acaacagcgc ggtgtggcgg atcatcgccg acggccaggt cggccgcacg tatctcattg
     1441 gcgcagaagg agaacgggac aacctgtcgg tgatgcgaac catccttcgg ctgatgggcc
     1501 gtgaccccga cgacttcgac cacgtcaccg accgggccgg ccatgacttg cggtacgcga
     1561 tcgacccatc accgctgtac cacgaactcg gctggggccc aaagcacacc gatttcgaag
     1621 aaggcctgcg tgccaccatc gagtggtacc gcgacaacga atcctggtgg cgcccactga
     1681 aagacgcggt cgaggccggc tacgaggagc gcggccagtg aaagcccgcg aattgagcgt
     1741 ccccggcgca tgggaaatca ccccacaggt gcacaccgac tcgcgtggcc tgttcttcga
     1801 atggttcacc gattcggggt tcaccgagtt cgccggacac cggctcgatc tgcgccaagc
     1861 caactgctcg gtgtcgtcgg ccggcgcgtt gcggggtgtg catttcgcgc agttgccgcc
     1921 gagccaggcg aagtacgtca cctgcctgac cggctcggtg ttcgacgtga tcgtcgacat
     1981 tcgggtcggc tcaccgacat tcgggaaatg ggacgcggtg gtgctcgacg atcaacatcg
     2041 ccgttcggtc tacctctccg agggcctggg ccatgcgttc ctggcgctgg aggatgattc
     2101 gacggtgatg tacctgtgct cggcggagta tgacccgggt cgcgagcaca ccatcaacgc
     2161 gctcgacccc gcgttggaca tcgcgtggcc cgctggagtc gaccagccga ttctctccga
     2221 ccgcgaccgc gacgcgccca gtcttgcgca ggcgcaatcc gcgggtctgc tgcccacgtg
     2281 ggaagagacg caggcgttcg tcgccaaact gcgatctcac atttgatcgt ccgcccgcgt
     2341 ctaccaggtg tgaaccgaat cgatgtgcag ttcgacgttg cgggcgacgc gtgcgccgca
     2401 tggaagtacg agcctgagac gacggactcg gacgacgcgc cgatcgtagt gatggcgcat
     2461 ggactctccg gtacccgacg tgatcgactg ggggcgttcg ccgagcgttt cgcacagcgc
     2521 aactgcttcg cgctcgtctt cgaccaccgt ggattcggag acagcggcgg tactccagat
     2581 ctgttcgagc ccgggcgtca gctcgaagac tggcggcacg cgattgcgtt cgcccgtcga
     2641 cgggccggcg tcgatgcgaa acgcgttgtc acatttggat cctcgatggg tggcggcaac
     2701 gcactggcgg cggccgtcca cgatggcgag gtggcggcgg taatcagcca ggtgccgttc
     2761 ctggacattc gccaggcata tcgggcgaag ccgcgcgttc gcgcgaggat gatgctcgcc
     2821 gcgagccgcg gtgagtacat gccggcggtg ggccagcctg acgaagcggc attcatcaac
     2881 gcacccgaaa gcgaggccgg ctggcgccac gtcgtcg
//
LOCUS       DASRJA010000167         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1286909,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000167 DASRJA010000000
VERSION     DASRJA010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286909"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..390
                     /locus_tag="VFC01_07875"
     CDS             70..390
                     /locus_tag="VFC01_07875"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HZN79588.1"
                     /translation="MPKFLIKASYNPDGVRGLIKEGGSKRRAVVQKLIEGLGGKLEAF
                     YYAYGADDAVIIADLPDAISGVALSLTVNATGAVRLETIPLITPEEIDAASKKSIDYK
                     APGA"
     gene            446..901
                     /locus_tag="VFC01_07880"
     CDS             446..901
                     /locus_tag="VFC01_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN79589.1"
                     /translation="MVKLTVNGTARTFDGDEDMPLLWYLRDDLRLTGTRFGCGAGLCG
                     ACTVHLDGLAVRSCQTPLRTLQNKRVVTIEGLSPDGRHPVQRAWVTHDVAQCGYCQTG
                     QIMQAASLLERTPNPTDQDIDRAMTGNICRCGTYQRIRAAIKTAAKERA"
     gene            898..>2193
                     /locus_tag="VFC01_07885"
     CDS             898..>2193
                     /locus_tag="VFC01_07885"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZN79590.1"
                     /translation="MSAIENVSRRRFIEGVFSTGAFVLAARVLPESAWAQAAAPAFRT
                     NADGAALNPSVYLGIQPDGTVFIVTHRSEMGTGIRTTLPMVAADELDADWGRVRIEQG
                     IGDARYGDQNTDGSRSIRDFYVAFRQAGASARSMLVSAAAAQWKVPASECTTRNHEVI
                     HEASNRRLAYGALVPAAAKLTVPKAESLQFKPKAAWRLVGQETSTYDQQDIVTGKAQF
                     GLDVFREGMVYASIEHPPVLGGTVKSVSDADARAVKGVQETVTLDTFKPPHLFQPLGG
                     VAVIANNTWAALQGRKKLKVDWNDGGHAAFESEAFKKQLLQTVSQPAKVARNVGNVDA
                     EFGKGGKLLEATYYTPLAAHASMEPPAAVADVRDGKATIWAPTQNPQAVQDAVSAVLG
                     IDKKNVICHVTLLGGGFGRKSKPDYAAEAAVLSRKLGKPV"
BASE COUNT          415 a          652 c          759 g          367 t
ORIGIN      
        1 aggtcgtgaa aatgggccct gaggcgcagc ctcgctagtg gtaccatcgt cggccgcagg
       61 aggtgcgaca tgccgaagtt cttgatcaag gcgtcctaca acccggacgg tgtccgtgga
      121 ctcatcaagg aaggtgggtc caagcggcgt gctgtcgtgc agaaactgat tgaaggcctg
      181 ggaggcaagc tcgaagcgtt ctattacgca tatggagccg acgacgcggt catcattgcg
      241 gaccttccgg atgcgatcag cggcgtggcc ctgagcctga ccgtcaacgc gacgggcgct
      301 gttcgcctgg agacgattcc gttgatcacg cccgaggaga tcgatgcggc aagcaagaag
      361 tcgatcgatt acaaggcgcc cggggcgtga ggccggggtt ggaagttggt agccgggagt
      421 tgggctttgg tggttgggag ctgacatggt caagctgaca gtgaatggaa ctgcccgcac
      481 tttcgacggc gatgaggata tgccgttgct gtggtatctg cgggacgatc tgcgactgac
      541 gggcacgagg ttcggctgcg gagccggctt gtgcggcgcg tgcacggtgc atctggacgg
      601 gctcgccgtt cgctcgtgcc agacaccgct gcgcacgctg cagaacaagc gcgtggtcac
      661 gattgaaggg ctgagcccgg atgggcgtca tccggtgcaa cgcgcctggg tcacgcacga
      721 cgtcgcgcag tgcggctact gtcagaccgg gcagatcatg caggccgcgt cactcctcga
      781 gcgaaccccg aaccccacgg atcaggacat cgatcgcgcc atgaccggca acatctgtcg
      841 atgcggcacc taccagcgca tccgcgccgc gatcaagacc gcggccaagg agcgggcatg
      901 agcgccattg aaaacgtcag tcgccgccgg ttcatcgagg gtgtgttctc gaccggtgcg
      961 ttcgtgctcg ccgcacgcgt gctgcccgaa tccgcgtggg cccaggcggc cgctccggcc
     1021 tttcgcacga atgccgatgg tgcagcgctg aacccgagtg tctatctcgg catccagccc
     1081 gatggcaccg tgttcatcgt cacgcatcgc tcggagatgg gcaccgggat ccgcacgacc
     1141 ctgccgatgg ttgccgcaga cgagctcgat gcggactggg gtcgcgtgcg aatcgagcag
     1201 ggcatcggtg atgctcgata cggcgaccag aatacagacg ggtcgcggtc gattcgcgat
     1261 ttctacgtcg cattccgaca ggctggcgcc tccgccaggt cgatgctcgt gagtgccgcg
     1321 gccgcgcagt ggaaagtgcc ggcctccgaa tgcaccacga gaaaccacga agtcattcac
     1381 gaggcgagca acaggcgact cgcatacggc gcgctcgttc cggccgccgc gaaactgacg
     1441 gtgccgaagg ctgagagcct ccagttcaaa ccgaaggctg catggcggct ggttggtcag
     1501 gagacgtcga cctacgatca gcaggacatc gtaaccggga aggcgcagtt cggcctcgac
     1561 gtgtttcgcg agggcatggt ctacgcctcg atcgaacacc cgcctgtgct cggtggcacg
     1621 gtgaagagcg tcagcgatgc ggacgcgcgc gcggtcaagg gcgtccagga gacggtcact
     1681 ctggacacgt tcaagccgcc gcatctcttc cagccactcg gcggtgtcgc ggtgatcgcc
     1741 aacaacacgt gggcagcgct gcagggtcgc aagaagctca aggtcgattg gaacgacggt
     1801 ggccatgcgg ccttcgagtc cgaggcattc aagaagcagc tgctgcagac ggtgagtcag
     1861 ccagcgaagg tcgcgcggaa tgtcggcaac gtggacgccg agtttggcaa gggtggcaag
     1921 ctgctcgagg cgacgtacta cacgccactg gcggctcacg catctatgga gccgccggcc
     1981 gctgttgcgg acgtgcggga tggcaaggcc accatctggg cgccgacgca gaatccgcag
     2041 gccgtgcagg acgccgtgtc ggcggtactc ggcatcgaca agaagaacgt catctgtcac
     2101 gtcacgctgc tcggcggtgg ctttggtcga aagtcgaagc cggactacgc cgcggaggcc
     2161 gcggtactgt cgcggaaact gggcaagccg gtg
//
LOCUS       DASRJA010000168         5218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2414237,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000168 DASRJA010000000
VERSION     DASRJA010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2414237"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VFC01_07890"
     CDS             <1..1056
                     /locus_tag="VFC01_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN79591.1"
                     /translation="VVDLGPGAGAAGGEVVFAGTVEALRESDTVTGRHLDDRAELKET
                     VRKPNGALEIRRASTHNLQDVDVDIPLGVLVVVTGVAGSGKSSLIHASVAGRDAVVAI
                     DQTPIRGSRRSNPATYTGLLEPIRKAFAKENGVKPALFSANSEGACPNCNGAGVIYTD
                     LGMMAGVATTCDVCEGKRFEASVLEYKFGGRDISEVLAMSVTEALAFFADGDAKVPAA
                     HKILDRLADVGLGYLSLGQPLTTLSGGERQRLKLATQMAEKGDVYILDEPTSGLHLAD
                     VEQLLGLLDRLVDSGKSVIVIEHHQAVMAHADWIIDLGPGAGHDGGRIVFEGTPGDLV
                     ADQSTLTGEHLADYVGA"
     gene            complement(1063..1431)
                     /locus_tag="VFC01_07895"
     CDS             complement(1063..1431)
                     /locus_tag="VFC01_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79592.1"
                     /translation="MSRVGKRRLLVVVASAPCASLVALVGGPGQASATPPDQPAPTIP
                     LPSNISALPLPVLPMPVGLPPFPYPADVPIVELPSDIPAVQLPSAIPRVELPADVPAV
                     QLPAEVPPIQLPAPVPPGLL"
     gene            1528..2187
                     /locus_tag="VFC01_07900"
     CDS             1528..2187
                     /locus_tag="VFC01_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZN79593.1"
                     /translation="MPSVLTVNLAHPRPNSDKGKLRTGIDKRPTNDAISVRAPGPMHD
                     GLGSGLVGDFIGDTQFHGGDDQAVYAYAREDLDVWQQELERDLGNGMFGENFTTAGVD
                     VTGSRIGERWHVGSDGVVLEVSAPRIPCRTFATWLSIQGWVKTFTKAATPGAYLRVIE
                     SGTVRAGDSIVVADRPDHDVTIGLVFRALTLEPDLLPGLLAADALPDEIKEQARRRTV
                     S"
     gene            complement(2190..2633)
                     /locus_tag="VFC01_07905"
     CDS             complement(2190..2633)
                     /locus_tag="VFC01_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79594.1"
                     /translation="MATRTAAQKRARAKVDYDAFLAQCPSRQLLDRISDKWVALILAA
                     LGSGGPHPGEIDYVDAPRPMRYSELSRRLAGVSQKMLTQTLRSLERDGLVIRTVTPTV
                     PVTVTYELTDLGLSLQQMMRGIKLWAEAHMDEVLANREEYDTRSA"
     gene            2751..3665
                     /locus_tag="VFC01_07910"
     CDS             2751..3665
                     /locus_tag="VFC01_07910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZN79595.1"
                     /translation="MTASTFRTAVVRTPAGPDSIEIIDVPVVEPGPGEVRVRIAAASV
                     NPVDLGVAGGIFHGMGLVNQPEHTGLGWDFAGTVEATGAGVDLEVGTRVAGMVAGFDR
                     DYGTYAEKLVVAAVDVAVVPDGLDLVAASTVPLNGLAAAQMLDLLGEPSADARLLVTG
                     AAGAVGSYLIKLAQDRGWHVTGLARPGDAEFVRGLGADFTADARPGWDAVADAAKLQE
                     RGLALVRDGGSFVGVQPGAEPDTERGITVAAVVSHPDGPRLGELLERTASGELPARVH
                     AVMPLDQVADAHRAMAKGGVRGRYVLKP"
     gene            complement(3711..4112)
                     /locus_tag="VFC01_07915"
     CDS             complement(3711..4112)
                     /locus_tag="VFC01_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZN79596.1"
                     /translation="MTTTLTIGELALQADVAATALRYYERIGLIHSPARVGGQRRYDD
                     SILARLDVIRLCKTAGFALDEIQLLFADDAPGRPASRALAETKLAEIDARIAELQRAR
                     QIIEWGMRCTCPSIDDCTCGIHTVKPFNSSL"
     gene            complement(4527..>5218)
                     /locus_tag="VFC01_07920"
     CDS             complement(4527..>5218)
                     /locus_tag="VFC01_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HZN79597.1"
                     /translation="AAWVELMGRLGYSKFAAHGGDWGGVITTVLGGRFPAHVLGIHTL
                     TPQAPPGLTTDGLTASERTWTEETRDFWGGPRAAFAKQQATRPQTIGYSLVDSPVGLL
                     AWILDKFAEWTDTEDSPFETISRDSILDNVTLYWLTRTGASSARIYYESHGGVNAFDP
                     ELRVDVPSAISVYPRDIEKCPRAWAQERFRQIVRWRSPETGGHFPSLEVPEYFVKDLQ
                     EGLTAVLAANR"
BASE COUNT          891 a         1621 c         1797 g          909 t
ORIGIN      
        1 gtcgtcgacc tcggccccgg cgcgggtgcc gccggcggcg aagtggtctt cgcgggcacc
       61 gtcgaggcgc tgcgggaaag cgacaccgtc accggccgac acctcgacga tcgggctgag
      121 ctgaaggaga cggtgcggaa acccaacggc gcgttggaaa ttcgtcgtgc cagtacacat
      181 aatctgcagg acgtcgatgt cgacatccca ctcggtgtgc tcgtcgtggt gaccggagtg
      241 gcgggctcgg gcaagagctc gctgatccac gcatcggtgg ccgggcggga cgcagtggtg
      301 gcgatcgatc agacgccgat tcggggctcg cgacgcagca accccgcgac gtacaccgga
      361 ctgctcgaac ccatccgcaa ggcgttcgct aaggaaaacg gtgtgaagcc agcgttgttc
      421 agcgcgaact ccgagggtgc ctgccccaac tgcaatggcg ccggcgtcat ctacaccgat
      481 ctggggatga tggccggcgt cgccaccacg tgtgacgtgt gcgagggcaa gcgattcgag
      541 gcatcggtgc tcgagtacaa gtttggcggt cgcgacatca gcgaggtgct cgcgatgtcg
      601 gtcaccgagg cgttggcgtt cttcgcggac ggcgacgcga aagtgcctgc tgcgcacaag
      661 atcctggatc gactcgctga tgttgggctc ggctatctgt cattgggtca gccgctcacg
      721 acgctgtccg gtggcgagcg gcagcgactc aagctggcta cgcagatggc cgagaaaggc
      781 gacgtgtaca tcctcgacga gccgaccagc ggcctgcacc tcgccgacgt cgaacagcta
      841 ctcgggttgc tcgaccggct cgtcgactcc ggcaagtccg tcatcgtcat cgagcaccac
      901 caggcagtca tggcgcatgc ggattggatc atcgaccttg gccctggggc cggccacgac
      961 ggcggccgga tcgtcttcga gggcacgcca ggtgatctcg tcgcggacca gtcgacgctc
     1021 actggtgagc acctggcgga ctacgtcggc gcctgacagc cgctacaaca agcctggcgg
     1081 gacgggcgcc ggcagctgaa tcggtggaac ttcggcggga agctggaccg cgggcacatc
     1141 ggcgggcagc tcgacgcgag gaatcgccga aggtaactgg accgcgggga tgtcgcttgg
     1201 cagttcgacg atcgggacgt ccgccgggta agggaatggg ggaaggccga cgggcatcgg
     1261 aaggacgggc agcggcagag ccgaaatgtt ggacggtagc gggatagtgg gcgccggttg
     1321 atccggcgga gtcgcagacg cctgtccggg tccgccgacg agcgcgacca gggacgcgca
     1381 gggcgcggac gcgaccacaa cgaggagacg ccgcttgccg acgcgggaca taccacaagg
     1441 ttagagcgtg tccgttcccc gttcgtcgaa gatggtcccg cttaattggt tgtgcaaggc
     1501 gcgtgaccgg tcagtacgct ccagcacatg ccgagcgtgt tgaccgtcaa tctcgcccac
     1561 ccgcgaccga attcggacaa gggtaagttg cggaccggga ttgataagcg gcccacgaac
     1621 gacgcgatta gcgtccgtgc tccaggacca atgcacgacg gtctcggcag tggtttggtt
     1681 ggcgatttca tcggcgatac gcagttccac ggcggcgacg atcaggcggt gtacgcgtac
     1741 gcacgcgagg acctcgacgt ctggcagcag gagctggagc gagacctcgg caacggaatg
     1801 ttcggcgaaa acttcaccac cgcaggggtc gatgtcaccg gttctcgtat cggcgagcga
     1861 tggcacgtgg gctccgacgg tgtggtgctt gaggtgtcag cgccccgtat tccgtgtcgg
     1921 acattcgcga cgtggctgtc gatccagggt tgggtcaaga ctttcaccaa ggcggcgacg
     1981 cccggtgcgt atctgcgagt catcgaatcc ggaacggtgc gcgccggcga ctcgatcgtc
     2041 gtagccgaca gaccggacca cgacgtgacg atcggcctcg tctttcgggc actgacgctg
     2101 gagcccgatc tgctgcctgg actcctggcg gcagacgcgt tgcccgacga gatcaaggag
     2161 caggctcgtc gccggaccgt ctcctaggtt caggcgctgc gggtgtcgta ctcctcgcga
     2221 ttggcgagca cctcatccat gtgggcctcg gcccacagct tgatgccgcg catcatctgc
     2281 tgaagcgaca gaccgagatc ggttagctcg tacgtgactg tgaccggcac ggtcggcgtc
     2341 acggtccgga tgaccaggcc gtcgcgctcg agcgagcgca gcgtctgggt aagcatcttc
     2401 tggctcaccc cggccaggcg tcgggacagc tcggagtagc gcatgggccg cggggcgtcg
     2461 acgtagtcga tctcgccggg atgcgggccg ccgctgccga gcgcggccaa aatcagcgca
     2521 acccacttgt cggagatccg gtcgagcagt tggcggctgg ggcattgcgc caggaacgcg
     2581 tcgtagtcca cctttgctcg tgcacgcttc tgagccgcgg tcctcgttgc catcgacggg
     2641 ccccttccta ccgagcggtg acctacgcac tttggagtgc gtacttccca acggagagta
     2701 acccatccaa agtggagcaa acagtcaatc acaccgagag gagcaacacc atgactgcct
     2761 ccaccttccg caccgcggtc gttcgcaccc cggccggacc cgattcgatc gagatcatcg
     2821 acgttcccgt cgttgagccc ggccccggcg aggtccgcgt ccggatcgcc gccgcgtcgg
     2881 tcaacccggt cgacctagga gtcgccggcg gcatcttcca cgggatgggg ctggtcaacc
     2941 agcctgagca caccggcttg gggtgggatt tcgctggcac cgtcgaggcc acgggcgccg
     3001 gcgtggacct ggaggtcggc accagggtgg ccggcatggt cgctggtttc gaccgtgatt
     3061 acggcaccta tgcggagaag cttgtcgtgg cggctgtcga cgtcgctgtc gtgccggacg
     3121 gactggatct ggtcgcggcg tcgacggtgc cgctcaacgg gctggccgcc gcgcagatgc
     3181 tcgacctgct cggcgagccg tccgctgacg ccaggctgct cgtgacaggg gcggctggcg
     3241 cggtcggcag ctacctcatc aagctcgcac aggaccgcgg ttggcacgtc accggtctgg
     3301 cgcgacccgg cgacgcagag ttcgtccgcg gcctcggcgc cgacttcacc gctgacgcca
     3361 ggccgggatg ggacgcggtc gccgatgctg cgaagctgca ggagcgcggc ctcgccctgg
     3421 tgcgcgatgg cgggtctttc gtcggcgtgc aaccgggtgc ggaaccggac accgagcgcg
     3481 ggatcaccgt ggctgccgtg gtgtctcacc ccgacggtcc gcgactgggc gagctgcttg
     3541 agcgcactgc gtccggtgag ctgccggctc gcgtgcacgc ggtcatgccg cttgaccaag
     3601 tcgcggatgc gcaccgtgcg atggcgaagg gcggggtgcg cggccgctac gtgctcaagc
     3661 cgtgattatg gaagctgttg ccgcccaggg agtttcatgg ccatgaattg tcacagtgac
     3721 gaattgaacg gcttgacggt gtggatgccg catgtgcagt cgtcgatgga cgggcaggtg
     3781 cagcgcatgc cccactcgat gatctgccgg gcgcgttgca gttcggcgat ccgggcgtcg
     3841 atctccgcca gcttggtctc agcgagggcc cggctggcgg gtcggccggg ggcgtcgtcg
     3901 gcgaacaaca actggatctc atcgagtgcg aagccggcgg tcttgcacag tctgatcacg
     3961 tcgagacggg cgaggatcga gtcgtcgtag cggcgttgac cgccgactcg ggcaggggag
     4021 tggatgagtc cgatccgctc gtagtagcgc agagcggtcg ccgcgacgtc cgcctgaagc
     4081 gccaattcgc cgatcgtcag agtggtggtc atggtcctcc tagctctggg gcttgactta
     4141 gagtcaactc taagtcgttg actgttggca tgcagaaacg cacacagcag atagacctca
     4201 gcgagttgag cgagaccgtg cgagcacttt catctaatgc aactcgcagt tagtttaaac
     4261 gccgataagc cacattatgt caggttgagg actccgtttc gagtgaccct cacctgccgc
     4321 ctgggccgca ctcggccaaa tgtactggag agcaccggtt ttcggtgaac agggaaacca
     4381 acccggcaat cacctcggcg ggtgtggctg tgatcgttcg gttgctgaca agtcgctaac
     4441 agcaccactc ggcggttcac cctctgtcgg cctgtaggac gattgccgga cggcctgcgc
     4501 atcgtcgcac tcagacccag ccgcgttcac cgattagcgg ccagcactgc cgtgaggcct
     4561 tcttgcagat ccttgacgaa atactcggga acctccagcg acgggaaatg tcccccggtt
     4621 tcgggcgacc tccatcggac gatctgtcgg aaccgctcct gcgcccaggc gcgcggacac
     4681 ttctcgatgt cgcggggata cacactgatt gctgacggga catcgacccg aagttcgggg
     4741 tcgaacgcgt tgacaccgcc gtggctttcg tagtagatgc gggccgacga tgcgccggtc
     4801 cgcgtcagcc aatacagggt gacgttgtca agaatgctgt ctctggaaat cgtctcgaac
     4861 gggctgtctt cggtgtcggt ccactcggcg aacttgtcga ggatccaggc gagaagcccg
     4921 actggtgagt cgacgagcga gtagccgatg gtctgcggtc gggtcgcctg ctgcttcgcg
     4981 aacgccgcgc gggggccgcc ccagaaatcg cgggtttcct cggtccatgt gcgctcggac
     5041 gccgtcagcc cgtccgttgt caacccgggc ggcgcctgcg gggtcagcgt gtggatgccg
     5101 agaacgtgcg ccgggaacct gccgccgagg accgtggtga tcacacctcc ccagtcgccg
     5161 ccgtgggctg cgaacttgct gtagccgagc cttcccatca gttccaccca tgcggccg
//
LOCUS       DASRJA010000169         5791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1007887,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000169 DASRJA010000000
VERSION     DASRJA010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007887"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VFC01_07925"
     CDS             <1..714
                     /locus_tag="VFC01_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN79598.1"
                     /translation="YLELKRGDNDQRLPAGGTIPLERTEPALDLDALIGGFRPVFRAL
                     DPDKVNNIAQSIITIFQGQGGTINDILDQTASLTSALADRDQAIGEVIKNLNTVLDTT
                     VKHQQQFDQTVQDFEKLITGLNNRADPIASSVADISDAAGTVGDLLADNRPLLQSTIT
                     YLEGVQQPLVDQKDQLNDILVRLPTAFKIIGRAGGIYGDFFNFYACDITLKTFGLQPG
                     GPVRTVKLTSQPSGRCTPK"
     gene            711..2294
                     /locus_tag="VFC01_07930"
     CDS             711..2294
                     /locus_tag="VFC01_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HZN79599.1"
                     /translation="MRTLEGSNRVRNGLMGIVVLLLVLGVGQSFASVPMLFATPTYYA
                     QFADTGGIGTGDKVRIAGVDVGRVQSMRIDGDKVVIGYSLGGTQIGTQSRASIRTDTI
                     LGRRNMEIEPRGTDPLRANGVLPLGQTTTPYQIYDAFFDVTKAASGWDTQTVKRSLNV
                     LSETIDQTYPHLSAALEGVERFSDTIGKRDDQIKQLLANANKIAGILGNRSEQINRLL
                     VNAQSLLAAINERQYAVSMLLERVAAFSEQVKGFIDDNPNLNHVLEQLRTVSGILRDR
                     RFDLMDTLSTVAKFVASLGEAVSSGPFFKVLLANLLPYQILQPFVDAAFKKRGIDPEE
                     FWRNAGLPAWRFPDPNAQRFDNGAPAPGPAVLEGTPEHPGPAVRAGTPCSYTPPPDGL
                     PRPDNPLPCAGLSVGPFGDNPYGPNYGPPDVATSDPNAHGPGFTPGVPAAAIPGQLPP
                     DMPGAPMPLPPAPPGARTVPVGPLPPEPPDFTPGIAPLPPALTGPPPPPGPGPVLAPA
                     DTPPLPGNPPYLPPGSQEG"
     gene            2298..3953
                     /locus_tag="VFC01_07935"
     CDS             2298..3953
                     /locus_tag="VFC01_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HZN79600.1"
                     /translation="MSQIFNIRNVKMPAVSRASVIIGTLVVILALVAAIVGYNLYKKL
                     TTNTVVAYFPETLALYAGDRVNIMGVKVGSIDKIEPAGDKMKVTFSYDNSFKVPANAT
                     ASILNPSLVASRNIQLSPPYTGGPVMEDNAVIPIDRTQVPVEYDELRDSINRILTDLG
                     PTPDQPKGPFGDIIESAANGFAGKGEQLNKTLNSLSEALYTLNEGRGDFFSVIKSLAL
                     FVNALYKSDQQFVALNDDLAQFTNAFTNTNREVANALQDLNELLTTTRQFINKNGEVL
                     TQDINNLADATNAIVQPDPLNGLETALHVFPTLGANLMNIVSPVTGGVMGIPVIANFA
                     NPMQLVCSAIQAGSRLGYQESAELCAQYLAPILDAIKFNFPPIGINQFVTAMTLPKQV
                     AYSEPRLQPPGGYKDTTVPGLFSRDTLFSHGNHEPGWVTAPGMQGIDVQPFTANMLTP
                     ECLGELMGGPDCAIPGAPAAFGVPPGGHLPGPSNAYDENNPLPPPWYPQPGPPPPPAP
                     GVIPGDPGGPSPLSGPAPGPGPGPAAPAGPAPAGPPLPAESGG"
     gene            3956..5101
                     /locus_tag="VFC01_07940"
     CDS             3956..5101
                     /locus_tag="VFC01_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HZN79601.1"
                     /translation="MRNWSRIASRALALVMVALVLSSCGSWKGIANVPLPGGPGTSSN
                     KMTIYVQMPETLALNVNSRVRVADVYVGRVREIALKNWVATLTLDLDPGVKLPVNTLA
                     KIGQTSLLGSQHVQLDAPPDPSPQLLRNGDTIPLKNSSAFPTTERTLASIATILRGGG
                     IQNLETIQTEINNLLTGRADQIREFLNRLDTFTDELNKQRQDITRAIDSTNRLLVIVG
                     QRNETLDRVLTEFPPLIKHFADTRDLFADAVEALGRISRAADGALAPSSANLHTNLQN
                     LQRPLKQLGRASPYLIGALKLMLTAPFSIENVPKVVRGDYLNVSLMVDITPSALDNGF
                     LSGTGISGTLRALEQSWGRDPETMIPDVRFTPNPHDAPNGPLVERGE"
     gene            5105..>5791
                     /locus_tag="VFC01_07945"
     CDS             5105..>5791
                     /locus_tag="VFC01_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZN79602.1"
                     /translation="MLTRFIKIQLVLFTILTVVALVVLGWYYLRLPSVAGVGTYDLHA
                     KLPRSGGLYSTANVTYRGTQIGKVIDVKPTETGAEATLRIDNRYKIPIDSVPNVHSVS
                     AIGEQYLDLVSTGNPGQYYQNGQMITQMGTVPSEVGPALDSANRGLAVLPKEKIDQLL
                     TETSNAVGGLGPALQRLVDSTTNIAQGFKDNLPQVDDIIAHSTPILQSQADSGDNIEQ
                     WSRNLNIIAAQ"
BASE COUNT         1090 a         1954 c         1793 g          954 t
ORIGIN      
        1 tacctcgagc tcaagcgtgg cgacaacgac cagcgactgc ccgcgggggg caccattccg
       61 ttggagcgca ccgagcccgc gctcgacctc gatgcgctga tcggcggatt caggcccgtg
      121 ttccgggcgc ttgaccccga caaggtgaac aacatcgccc agtcgatcat cacgatcttc
      181 cagggacagg gcggcacgat caacgacatc ctggaccaaa ccgcgtcact gacgtccgcg
      241 ctggcggatc gcgatcaggc catcggcgag gtgatcaaga acctgaacac cgtgctggac
      301 acgacggtca agcatcagca gcaattcgac caaacggtgc aggatttcga gaagctcatc
      361 acagggctca ataaccgtgc cgacccgatc gccagttcgg tcgccgacat cagcgacgcg
      421 gcgggcaccg tcggcgatct gcttgccgac aaccgcccgc tgctgcaaag caccatcact
      481 tatctcgagg gggttcagca gccgcttgtg gatcagaagg atcagctcaa cgacatcctg
      541 gttcggctgc cgaccgcgtt caagatcatc gggcgggccg gcggtatcta tggcgacttc
      601 ttcaacttct atgcctgcga catcacgttg aagactttcg gtttgcagcc cggcgggccg
      661 gtccgcaccg tcaagctcac gtcgcagcca tccggtaggt gcacgccgaa atgaggacac
      721 tcgagggttc gaaccgcgta cgaaacgggt tgatgggcat agttgtccta ctcctcgttc
      781 ttggtgtcgg acagagcttc gccagcgtgc cgatgctgtt cgcgacgccg acctactacg
      841 cccagttcgc cgacaccggc ggcatcggca ctggcgacaa ggtgcgcatc gccggcgtgg
      901 atgtcggacg agtgcaatcc atgcggatcg acggcgacaa ggtcgtcatc ggctattcgc
      961 tcggtgggac ccagatcggc acacagagcc gcgcgtccat ccgcacggac acgatcctgg
     1021 gccggcgcaa catggagatc gagccgcgtg gcacagatcc gttgcgcgcc aacggcgttc
     1081 tgccactcgg gcagaccacg acgccgtatc agatctacga cgcgttcttc gacgtcacca
     1141 aggcggcgtc cggctgggac acccagacgg tgaagcggtc actgaatgtg ttgtcggaga
     1201 ccatcgatca gacgtatcca catctgtccg cggcgctcga gggcgtggaa cggttctccg
     1261 acacgatcgg caagcgcgac gaccagatca agcaactgct ggccaacgct aacaagatcg
     1321 cgggcattct gggcaaccgc agcgagcaga tcaatcggtt actggtcaac gcgcagtcac
     1381 tgttggcggc gatcaacgaa cgccagtacg cggtcagcat gctgctggag cgggtggcgg
     1441 ccttttccga gcaggtgaag ggtttcatcg acgacaaccc aaacctcaac cacgtgctgg
     1501 agcagctgcg caccgtcagc ggcatcctgc gcgaccgcag gttcgacctg atggacacgc
     1561 tgtccaccgt ggccaaattc gttgcctccc ttggtgaggc ggtctcgtcg ggtccgttct
     1621 tcaaagtgtt gttggcgaac ctgctgccct accagattct gcaaccgttc gtcgacgccg
     1681 ctttcaagaa gcgcggcatc gacccggagg agttctggcg caatgctggc ctgccggcct
     1741 ggcggttccc cgatccgaat gctcagcggt tcgacaacgg tgctccggcg ccggggccgg
     1801 cggtgctcga aggcacgccc gaacatcctg ggcccgcggt gcgggcgggc acgccgtgct
     1861 cctacacgcc gccgcccgac ggcctgccaa ggcccgacaa cccactgccg tgcgcgggcc
     1921 tgagcgtcgg ccccttcggc gacaatccgt acggccccaa ctacggcccg cccgacgtgg
     1981 cgacatccga tccgaatgca cacgggcctg gcttcacacc gggcgtaccc gccgcggcca
     2041 tccccggtca attgccgccg gatatgcccg gcgctcccat gccgctgccg cctgcgccgc
     2101 ccggtgcgcg caccgtgccg gtgggcccgc tgcctcctga gccgccggac ttcacgcccg
     2161 gcatcgcgcc gttgccgccg gccctgacgg gtccgccgcc gccgcccgga ccgggtccgg
     2221 tgctggcgcc ggctgatacc ccgccgctgc caggcaaccc tccgtacctc ccgcccggtt
     2281 cgcaggaggg ctgatcgatg tcgcagatct tcaacatccg aaacgtgaag atgccggccg
     2341 tgtctcgggc ctcggtcatc atcggcacgc tggtcgtgat cctggcgctg gtggccgcca
     2401 tcgtcggcta caacctgtac aagaagctga ccaccaacac cgttgtcgcc tacttccccg
     2461 agacccttgc gctgtatgcc ggcgacaggg tgaacatcat gggcgtcaag gtcggctcga
     2521 tcgacaagat cgagcctgcc ggcgacaaga tgaaggtgac cttcagctac gacaacagct
     2581 tcaaggttcc ggccaacgcc acagcatcga tcctcaaccc cagcctggtg gcgtcgcgca
     2641 acatccagct gtcgcccccg tacaccggcg gaccggtgat ggaggacaac gcggtcatcc
     2701 cgatcgaccg cacccaggtg cccgtcgagt acgacgagtt gcgcgactcg atcaaccgga
     2761 tcctgaccga cctcggtccg acaccggatc agcccaaggg cccgttcggt gacatcatcg
     2821 aatccgcggc caacgggttc gcaggcaagg gcgaacagct caacaaaacg ctgaattcgt
     2881 tgtcagaagc cctctatacc ctgaacgagg gccggggcga cttcttcagt gtgatcaaga
     2941 gcctggcgct gttcgtcaac gcgttgtaca agagcgatca gcagttcgtc gcactcaacg
     3001 acgatctcgc gcagttcacc aacgcgttca ccaacacgaa ccgggaagtg gccaacgcgc
     3061 tgcaggacct caacgagttg ttgaccacca cccgtcaatt catcaacaag aacggcgagg
     3121 tgctgaccca ggacatcaac aacctggccg atgcgaccaa cgcgatcgtg caacccgatc
     3181 cgctcaacgg tttggaaact gccctgcacg tgttccccac gctcggcgcg aacctgatga
     3241 acatcgtctc gccggtgacc ggtggtgtca tgggcatccc cgttatcgcg aactttgcga
     3301 acccaatgca gttggtctgc agcgccattc aggcgggtag ccggctgggg tatcaggagt
     3361 ccgccgagct gtgtgcgcaa tacctggcac cgatcctcga cgcgatcaag ttcaacttcc
     3421 ccccgatcgg tatcaaccaa ttcgtgaccg cgatgacgct gcccaagcag gttgcgtact
     3481 cggagccacg cctgcagccg ccgggcggct acaaggacac caccgtgccc ggcctcttct
     3541 cacgcgacac cttgttctca cacggcaacc acgagccggg ctgggtcacc gcgcctggca
     3601 tgcagggcat cgacgtgcag ccgttcaccg cgaacatgct gacgccggag tgcctcggcg
     3661 agctgatggg cggacccgac tgcgcgattc cgggggcgcc ggccgcgttc ggcgtgccgc
     3721 ccggcgggca cttgccaggg ccgtccaacg cgtatgacga gaacaacccg ctgccgccgc
     3781 cgtggtatcc gcagccggga ccgccgcctc ctcccgcacc gggcgtcata cccggcgatc
     3841 cgggtggtcc gtctccgctg tccgggcctg cgccggggcc cggccccgga ccggccgccc
     3901 ctgccggacc cgcgccggcg ggaccaccgc tacccgctga gtcaggtgga taagcatgcg
     3961 taactggagc cgtatcgcca gccgtgccct cgcgctggtg atggtcgcgc tggtactgag
     4021 ctcgtgcggg tcgtggaagg gcatcgcgaa tgttccgctg cccggcgggc ctggcacgtc
     4081 atccaacaag atgacgatct atgtgcagat gccggagacg ttggcgctca acgtcaatag
     4141 ccgggtgcgg gtggccgacg tctacgtcgg ccgggtgcgt gagatcgcac tcaagaactg
     4201 ggtcgcgacg ctcacattgg atctggaccc cggcgtcaag ctgccggtga acacgctggc
     4261 caagatcggc cagaccagcc tgctgggcag ccagcacgtg cagttggacg cgccgccgga
     4321 tccgtcgccg cagctgttgc gcaatggtga caccattccg ctgaagaaca gctcggcgtt
     4381 cccgactacc gagcggacgc tggcgagtat cgccaccatc ctgcgcggcg gcggcatcca
     4441 gaatctggaa accatccaga ccgagatcaa caacctgctg accggtcggg ccgatcagat
     4501 ccgggagttc ctgaatcggt tggacacgtt caccgatgag ctgaacaagc agcgccagga
     4561 catcacccgg gcgattgatt ccaccaatcg gctgctggtg atcgtgggcc agcgcaacga
     4621 aaccctggac cgggtgctga ccgaattccc gccgctgatc aagcatttcg ccgacacccg
     4681 ggatctgttc gccgacgcgg tggaggcgct tggtcggatc agcagggcgg ccgacggcgc
     4741 cctggctccg tcgagcgcca acctgcacac caacctgcag aatctgcagc ggccactcaa
     4801 gcagttgggc agggcgtcgc cgtacctgat cggtgcgctc aagctgatgc tgacggcgcc
     4861 gttctccatc gagaacgtgc cgaaggtggt gcgcggcgac tacctgaatg tgtcgctgat
     4921 ggtcgacatc accccttcgg cgttggacaa cgggttcttg tccggcaccg gtatctcggg
     4981 cacgctgcgc gcactcgagc agtcctgggg tcgtgatccg gagacgatga tcccggatgt
     5041 gcggttcacg ccgaacccgc acgacgcgcc gaacggaccg cttgtggaaa ggggtgagtg
     5101 agcagtgctg acccgtttca tcaagatcca gctggtgctg ttcaccatcc tcaccgtagt
     5161 cgcgctcgta gtactgggct ggtactacct gaggctgccg agcgttgccg gcgtcgggac
     5221 ctacgacctg catgccaagc tgccccggtc cggcggcttg tactcgactg cgaacgtgac
     5281 gtatcgaggc acgcagatcg ggaaggtgat cgacgtcaag cccaccgaga cgggggcgga
     5341 ggccacgctt cggatcgaca accgctacaa aatcccgatc gactcggtac ccaacgtgca
     5401 ctccgtgtca gcgatcggcg agcagtacct ggacctcgtg tcgacgggta acccggggca
     5461 gtactaccag aacggtcaga tgatcacgca aatgggcacg gtgcccagcg aggtcggtcc
     5521 ggcgctggac tccgcgaatc gcgggctggc ggtgctgcca aaggagaaga tcgatcagct
     5581 gctcaccgaa acctccaacg ccgtaggcgg attgggcccg gcgctgcagc ggttggtcga
     5641 ctcgacgacg aacatcgcgc agggcttcaa ggacaacctg ccgcaggtcg acgacatcat
     5701 cgcgcactcg acgccgatcc tgcagagcca ggccgactcc ggcgacaaca tcgagcagtg
     5761 gtcacgcaac ctgaacatca tcgcggccca g
//
LOCUS       DASRJA010000170         5070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_442420,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000170 DASRJA010000000
VERSION     DASRJA010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442420"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VFC01_07950"
     CDS             <1..1410
                     /locus_tag="VFC01_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79603.1"
                     /translation="RAAGQAELASAQIEFVAIADVEMRLDGDPIALEAGGTCAASVSA
                     PTEIELPGLLRARVVPGTPASDSQAKLEAAQEVLVAALTKVRAADVAEARRLDDRRRE
                     LIAAREKLHATSEALVGDDSVDELRTRLAELREAEPAVQGLWDTVDPTVARADLDAAI
                     AAHKQAMADCETHRKVAEAAAKRLGERTSRANVAREKLVAAEGELTAARERLTLQRST
                     AGDDDLAVKAEADGEEARRATGLVAELGAELARSAPDTVTAALNDAVRRADSLDNRHI
                     DVSEALREVTTQLKVYGTEGRKGQLDAAQTEREHAEGDYLRMHRRARAAQLLRSVMVR
                     HRDATRLRYVDPFRNQVERLGRIVFGDSFEVEIDSALRICSRTLSGRTVPYESLSGGA
                     KEQMGIVARLAGAALVAKEDSVPVVIDDALGFSDADRLIKMGSVFNTVGGDGQVIVLT
                     CSPERYASVTGAHHIELTA"
     gene            1469..2278
                     /locus_tag="VFC01_07955"
     CDS             1469..2278
                     /locus_tag="VFC01_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HZN79604.1"
                     /translation="MGAELSASEVRGVAGRSDIWLPVALLILAGLGWWWSAVSAGDMA
                     GDAVPMDGMPMDGMPMEPQPAMAMSFVAFLVAWVAMMAAMMFPAVLPVVRLYARAAAN
                     GQAAPVAVFVGGYIVLWAVVGIPAFFAWSRLNEPLMQGSSWAGRVAGAVAVAAGLYQL
                     TPLKSVCLRHCRSPLSFFMRHGKHLDRPTGAFVAGVRHGMFCVGCCWMLMALLIAFGA
                     MQLVWMLVLAVLILLEKIAPFGEQLARVTGAALLVLGVALLVRPDFVTHLV"
     gene            2293..2919
                     /locus_tag="VFC01_07960"
     CDS             2293..2919
                     /locus_tag="VFC01_07960"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HZN79605.1"
                     /translation="MTTTEQRTRWFLKGRGYEFCNCAPGCGCNFHGFPTSADGSCKAM
                     VGNHILEGSCGDVDLSGVKAIAVIDWPKAIHDGNGKAVFIVAPETTDEQIECLSQIYT
                     GQLGGDPWGILGTTYEVVGLAKAPISFEGTGVTATMTAEGLGRATGDTFKNPVTGEPH
                     IAHIVLPDGFIWKKGECGIGTFQVDAEGLRLGFTDTNWIFYEFDWSNA"
     gene            complement(3114..3776)
                     /locus_tag="VFC01_07965"
     CDS             complement(3114..3776)
                     /locus_tag="VFC01_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79606.1"
                     /translation="MPVPRSYQQNCPIAKGLDVLGERWTLLILRELIGGPRRYGDLRA
                     QLPGIATNLLADRLRELEDAGLVDREELPPPVARTVYTLSGTGWRKVLPVVKAIATFG
                     LDMVVPDESALTPLNGFLAGILLGIDTGLAAELNASYRIEIDDRRFDFEVDGAGLAAA
                     KGAPAVTVTATAGDLITARLGATAAERKAALRRVKFDGDAGAVDAMRRVFGLSGDPGL
                     VA"
     gene            3849..>5070
                     /locus_tag="VFC01_07970"
     CDS             3849..>5070
                     /locus_tag="VFC01_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZN79607.1"
                     /translation="MSTQTPVFKDHPVAALVFICLSVFVISVDATIVNVALPTLSREL
                     DADTAQLQWIVDAYTLVMAGLMLSMGSLSDRFGRRGWLSAGLALFAITSVYAAQVDSA
                     DALIWARAAMGVGAAVIFPATLSLITNIFVDPVKRAKAIGLWAAMVGVGVAAGPISGG
                     WLLEHFSWGSVFMVNVPIAAVAIIGGVLFIPTSRDPAAPPVDVPGLILSSVGITAVVY
                     TVIEAPNWGWRSAHTAIGFAVGAVVLAVFALWERRTPHPMLDVSVFVNRRFSGGSLAV
                     TAGFLTLFGFIFVITQYFQFIKGYSAFETGVRLLPVAISIAVASIVGPRIVERIGTTA
                     VVAGGLVTFAAGLAWASTADAATPYIQIALQMVLLGGGLGLTTAPATESIMGSLSADK
                     AGVGSAVNDTTRELG"
BASE COUNT          804 a         1638 c         1726 g          902 t
ORIGIN      
        1 agggctgccg gtcaagccga actcgcctct gcccagatcg aattcgtcgc gatcgccgac
       61 gtcgagatgc ggctcgacgg tgatccgatc gccctcgagg ccggcggcac ctgcgccgcc
      121 agtgtcagcg cgccgaccga gatcgaactg ccgggtctgc tgcgtgcgcg ggtggtgcct
      181 ggcaccccgg catcggattc acaggcaaaa ctcgaagcgg cccaagaggt tttggtggca
      241 gcgctgacga aggtgcgggc cgccgatgtt gccgaggcgc ggcgcctgga tgaccgccgt
      301 cgcgagttga tcgctgctcg cgagaagctg cacgccactt ccgaagctct ggttggcgac
      361 gacagcgtcg acgaattgcg aacgcggctg gccgaattgc gcgaagccga gcccgccgtg
      421 cagggcctct gggataccgt ggatccgacc gtcgcccggg ccgatctcga cgcggcgatc
      481 gccgcacaca agcaagccat ggcggattgc gagacgcatc gcaaggtcgc ggaagccgcc
      541 gcaaagcggc tgggggagag gacgtcacgc gccaacgtcg cccgcgagaa gctagtcgcc
      601 gctgaagggg aactgaccgc cgcacgtgag aggctgacgc tgcagcgttc gacggcgggt
      661 gatgacgatc tcgcggtcaa ggcggaggcc gacggtgagg aggcccggcg cgcaaccggc
      721 ctcgtcgccg aactgggcgc tgaattggcg cgcagcgcac cggacaccgt taccgccgcg
      781 ctgaatgatg ctgtccgccg ggccgattcg ctggacaacc ggcacatcga cgtgtccgaa
      841 gcgctgcgcg aggtgaccac acagctcaag gtctacggca ccgagggccg caaggggcaa
      901 ctggacgccg cccaaaccga acgcgagcac gccgaagggg actatctgcg catgcatcga
      961 cgcgctcgcg ctgcgcaact gctgcgttcg gtgatggtgc gccaccgcga cgccacccgg
     1021 ctgcggtatg tcgacccgtt ccgcaaccag gtggagcggc tgggccgcat cgtgttcggc
     1081 gacagcttcg aggtcgagat cgacagcgcg ctgcgcatct gcagccggac actgtcaggc
     1141 cgcaccgttc cgtacgagtc gctgtcgggc ggcgcgaagg agcagatggg catcgtcgcg
     1201 cggttggcgg gggcggccct ggtggccaag gaggacagtg ttcccgtggt catcgacgac
     1261 gcgctcggct tctccgacgc tgaccggctg atcaagatgg ggtcggtgtt caacaccgtc
     1321 ggtggcgacg gccaggtgat cgtgttgacc tgcagccccg agcggtatgc cagcgtgacg
     1381 ggcgcgcacc acatcgagct gacagcgtag gcgggcaatc cgccgcctcg gccgccgccg
     1441 ggtgccatag ttggtcaggg acgacgcggt gggggccgag ttgagcgcga gcgaagttcg
     1501 tggggtagcg ggcagaagcg acatctggct gcctgtcgcg ctgctgatcc tggcgggtct
     1561 cggttggtgg tggtctgccg ttagcgccgg tgacatggcc ggcgacgcgg tgccaatgga
     1621 cggcatgcca atggacggca tgccgatgga gccgcagccc gcgatggcga tgtcgttcgt
     1681 cgcattcctg gtggcgtggg tggcgatgat ggcggcgatg atgtttccgg cggtcctgcc
     1741 cgtggtccgc ctctacgcca gggccgcagc caacggtcag gcggcgccgg tggcggtctt
     1801 cgtcggcggg tacatcgtgc tgtgggccgt cgtcggcatc cccgcgttct tcgcgtggag
     1861 tcggctgaac gagcccctca tgcagggcag ctcatgggcc gggcgcgttg caggcgcggt
     1921 ggcggtcgct gcagggctgt accaactcac gccgctgaag tcagtgtgcc tgcgccactg
     1981 ccgatcacca ctctcgttct tcatgcgcca cggtaagcat ctcgaccgtc cgactggcgc
     2041 attcgttgcc ggtgtccgcc acgggatgtt ctgcgtcggt tgctgttgga tgttgatggc
     2101 gctgctaatc gcgttcggcg caatgcaatt ggtgtggatg ctggttctcg cggtcctgat
     2161 cctattggag aagatcgcac cgtttggtga acagcttgcg cgcgtgacgg gcgcggcgct
     2221 gttggttctg ggtgtcgcgc tgctggtgcg ccccgacttc gttacccatc tcgtctgatc
     2281 gaaaggcatg ccatgacaac gactgaacaa cgcacccggt ggtttctcaa ggggcgcgga
     2341 tacgagttct gcaactgtgc accggggtgc ggctgcaact ttcacgggtt tccgacgtcc
     2401 gcggacggca gctgtaaggc catggtcggt aaccacatcc tcgaggggtc gtgcggtgac
     2461 gtggacctgt ccggcgtgaa ggcgatcgcg gtcatcgact ggccgaaggc gattcacgac
     2521 ggcaacggca aggcggtgtt catcgtcgcg cctgaaacca ccgacgagca gatcgagtgc
     2581 ttgtcgcaga tctataccgg ccagctcggc ggcgacccgt gggggatcct cggcacgacg
     2641 tacgaggtcg tcgggctcgc gaaggcgccg atcagtttcg agggcaccgg cgtgacggcc
     2701 acgatgacgg ccgagggcct cgggcgtgcc accggcgaca cgttcaagaa cccggtgacc
     2761 ggcgagccgc acatcgctca catcgttctg cctgacggct tcatctggaa aaagggtgaa
     2821 tgcggcatcg ggacgttcca ggtcgacgct gagggccttc gtctcggctt caccgacacc
     2881 aactggatct tctacgagtt cgactggtcg aacgcctagg ggacggcggc gcttgctgat
     2941 aagcgcgtgg ccgttgtgaa gattgcacag agggttgcaa cttccgccct attcacagcc
     3001 ctgaatgcaa tcttgatggc gtgactgcgt cggcggctgc cccggcgacg ttgactctgc
     3061 gtaccgggcg tgggccactc gtactttcgc gccctagccg caaggtcaac gcactaagcc
     3121 accaggcccg gatctccgga taacccgaac acgcgtcgca tcgcgtcgac tgcaccggcg
     3181 tcgccgtcga acttcacccg gcgcagcgcg gccttgcgtt cggcagcagt cgcacccagg
     3241 cgcgcggtga tcaggtcgcc ggccgtcgcg gtcacggtca cggcgggcgc acccttcgcg
     3301 gcagccagcc cggcgccgtc gacttcgaaa tcgaaccggc gatcgtcgat ctcaatgcgg
     3361 tacgaggcgt taagctcggc cgccagcccg gtatcgatgc ccagcaagat gcctgccagg
     3421 aacccgttca gcggcgtcag cgcgctctcg tcgggcacca ccatgtcgag tccgaacgtc
     3481 gcgatggcct tcaccaccgg cagcactttg cgccagcccg tcccgctcag cgtgtagaca
     3541 gttcgcgcga cgggtggggg aagttcctcg cgatcgacga gaccggcgtc ctcgagctcg
     3601 cgaagccggt cggccagcag gttcgtggcg atgccgggca gctgtgcgcg caggtcaccg
     3661 taccggcggg ggccgccgat gagttcccgc aggatcagca gcgtccatcg ctcgccgagc
     3721 acgtcgaggc ccttggcgat cgggcagttc tgctgatagc tcctaggcac gggaataact
     3781 ataccactca gacaagtatc tagtcatatc acttgaaaaa catgtctgaa aggtttacgg
     3841 ttctgtccat gagtacccaa actccagttt tcaaagacca cccggtcgcc gcgcttgtct
     3901 tcatctgcct gagcgtcttc gtcatcagcg tcgacgccac catcgtcaac gtcgccctgc
     3961 ccacgctgtc gcgggaactg gacgccgaca ccgcacagct gcaatggatc gtcgacgctt
     4021 acacactggt catggccggt ctaatgctgt cgatgggcag cctgagtgac cgcttcggcc
     4081 gacggggctg gctcagcgcc gggctcgccc tctttgcgat cacatctgtc tatgccgcac
     4141 aggtggattc ggcggacgcc ctgatctggg cgcgcgcggc catgggcgtc ggcgccgcgg
     4201 tgatcttccc ggccacgctc tcgctgatca ccaacatctt cgtcgatccc gtcaagcggg
     4261 cgaaggcgat cgggctgtgg gcggcgatgg ttggcgtcgg cgtggccgca ggcccgatct
     4321 cgggcggctg gctgctcgaa cacttctcgt ggggctcggt gttcatggtc aatgtgccga
     4381 tcgccgcggt ggccatcatc ggcggtgtcc tgttcatccc gacctcgcgc gacccggcgg
     4441 caccaccggt cgacgtgccc ggcttgatcc tgtcctcggt cggcatcacc gcggtggtct
     4501 acaccgtcat cgaggcgccc aactgggggt ggcgcagcgc gcacaccgcc atcgggttcg
     4561 cggtaggcgc cgtcgtgctc gccgtcttcg cgctgtggga gcggcgcacc ccgcatccga
     4621 tgctcgacgt gtcggtgttc gtcaaccggc ggttctccgg cggaagcctt gcggtcacgg
     4681 caggttttct gaccttgttc ggcttcatct tcgtcatcac ccagtacttc caattcatca
     4741 agggctacag cgcattcgag accggcgtcc ggttgttgcc tgtcgcgatt tcgattgcgg
     4801 tcgccagcat cgtcggaccg cgcatcgtcg aacgcatcgg caccaccgcg gtggtcgccg
     4861 gcggcttggt caccttcgcg gcaggactcg catgggcatc gaccgccgac gccgctacgc
     4921 cgtacatcca aatcgccttg cagatggtgc ttctcggcgg cggcctggga ctcaccacgg
     4981 cgccggccac cgagtcgatc atgggctcgc tgtccgccga caaggccggc gtcggttcgg
     5041 ccgtgaacga caccactcgc gaactgggcg
//
LOCUS       DASRJA010000171         4709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1284987,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000171 DASRJA010000000
VERSION     DASRJA010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284987"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFC01_07975"
     CDS             complement(<1..237)
                     /locus_tag="VFC01_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79608.1"
                     /translation="MNSKKSVAHRLGRVLETVTRQSGRLPDTPEFGSWLLGKSTESPA
                     RRRVRIQVILTVLILATNMLGIGIAILLVTVVFPV"
     gene            284..955
                     /gene="nucS"
                     /locus_tag="VFC01_07980"
     CDS             284..955
                     /gene="nucS"
                     /locus_tag="VFC01_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HZN79609.1"
                     /translation="MRLVIAQCTVDYVGRLTAHLASARRLLLFKADGSVSVHADDRAY
                     KPLNWMSPPCWLSEELDGPVPVWVVENKSGEQLRITVEDVEHDSSHELGVDPGLVKDG
                     VEAHLQALLAEHVEVLGTGYTLVRREYMTAIGPVDLLCRDEQGRSVAVEIKRRGEIDG
                     VEQLTRYLDLLNRDTVLAPVSGIFAAQQIKPQARTLATDRGIRCVTLDYDAMRGMDSD
                     EFRLF"
     gene            968..1264
                     /locus_tag="VFC01_07985"
     CDS             968..1264
                     /locus_tag="VFC01_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79610.1"
                     /translation="MPRRRNPPRRQQPLAPLPAARRIEAGPDGYDYEVRPIPASRATK
                     IYRCPGCDHEIVIGTAHLVVWPTDLGERAVDERRHWHTPCWTHRATRSPTRRWS"
     gene            complement(1269..1739)
                     /gene="mce"
                     /locus_tag="VFC01_07990"
     CDS             complement(1269..1739)
                     /gene="mce"
                     /locus_tag="VFC01_07990"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HZN79611.1"
                     /translation="MTAEQVDARPLLATALVTAIDHIGIAVADLDAAIKWYHDHLGMI
                     VLHEEVNEDQGVREAMLSVRGAPVGSAQLQLMAPLDETSTIAKFLDKRGPGLQQLAYR
                     VSDIDALTERLREQGVRLLYEAPRRGTADSRINFIHPKDAGGVLVELVEPAAEH"
     gene            1822..3003
                     /locus_tag="VFC01_07995"
     CDS             1822..3003
                     /locus_tag="VFC01_07995"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZN79612.1"
                     /translation="MTTSVIVAGARTPVGKLMGSLKDFSGSDLGAIAISGALEKAGVP
                     ASLVEYVIMGQVLTAGAGQMPARQAAVAAGIGWDVPSLTINKMCLSGIDAIALADQLI
                     RAGEFDVVVAGGQESMTKAPHLLMDSRAGYKYGDVTVLDHMAYDGLHDVFTDQPMGAL
                     TEHRNDVDQFTRAEQDEFAAQSHQKAAAAWKDGVYADEVVPVKIPQRKGDPIEFTEDE
                     GIRANTTAESLAGLKPAFRKDGTITAGSASQISDGAAAVVVMNKAKAQELGLTWLAEI
                     GAHGVVAGPDSTLQSQPANAIKKAIAREGISVDQLDVIEINEAFSAVALASMKELGVD
                     PKKVNVNGGAIAIGHPIGMSGARITLHAALELARRGSGYAVAALCGAGGQGDALILRA
                     G"
     gene            3048..3431
                     /locus_tag="VFC01_08000"
     CDS             3048..3431
                     /locus_tag="VFC01_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HZN79613.1"
                     /translation="MAPMTPAFDLRSAAGWFRLIALAEAASWVGLLIGMYLKYLGTPR
                     TEIGVKVFGMAHGLIFIAFVVVGLLVGIAFKWAAGTWLLALLASIVPLCSVIFLIWAD
                     RTGRMGLTEATSAVAQPGRRAPETT"
     gene            3445..4338
                     /locus_tag="VFC01_08005"
     CDS             3445..4338
                     /locus_tag="VFC01_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN79614.1"
                     /translation="MSRPRPPVAASMAGAVDLSALKQRAAPAGEGAAASPSGVEITEA
                     NLEAEVLVRSSQMPVVVLLWSPRSEASAQLGDVLGQLAATDGGKWSLATVNVDAVPRV
                     AQMFGVQAVPTVVALAAGQPISSFQGVQPPEQLRRWVDSLLNATAGKLGGAADGDEPE
                     QVDPELAKAREHLDGGDFDAALKAYQALLDANPNHAEAKGAVRQIGFLQRATSRAPGA
                     IVAADAKPDDIELAFAAADVEILQQSVASAFDRLIALIKRTAGDERTKVRTRLIELFD
                     LFDPADPEVIAGRRNLANALY"
     gene            complement(4409..>4709)
                     /locus_tag="VFC01_08010"
     CDS             complement(4409..>4709)
                     /locus_tag="VFC01_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HZN79615.1"
                     /translation="WIDANDSANNVLSFLRYGDDGSVMACVFNFSGSEHSRYQLGLPH
                     AGTWREVLNTDADLYNGTGIGNYGAVQATDEPWHGRPASAVMVLPPLAALWFEPA"
BASE COUNT          796 a         1495 c         1605 g          813 t
ORIGIN      
        1 gaccgggaac acgacggtga ccagcagtat ggctatgccg atgccgagca tgtttgtggc
       61 caggatcaac accgtcagga tgacctgaat gcggacccgc ctgcgtgccg ggctttcagt
      121 ggatttgcct agcagccagg agccgaactc aggcgtatcg ggcagccgac cgctctgccg
      181 ggtgactgtc tccaggaccc ggcccagccg atgcgcgaca ctcttcttgg agttcatcgt
      241 gggcccagcc taatttggcg gtgccggcac ataaggtgtt cgggtgcgtc tcgtgatcgc
      301 tcagtgcacc gttgactacg tcgggcggct cacagcccat ctggcctccg cccgccggct
      361 gctgctgttc aaggccgacg gctcggtcag cgtgcacgcc gacgaccggg cgtataaacc
      421 gctcaactgg atgagcccgc cctgctggct gtccgaggaa ctcgacgggc cggtgcccgt
      481 gtgggtcgtc gagaacaagt cgggcgagca gctgcgcatc acggtggagg acgttgagca
      541 cgattccagc cacgagctcg gcgtcgatcc cggtctggtc aaggatggcg tcgaagccca
      601 cctgcaggcg ctgctggccg aacacgtcga agtgctcggc actggctaca cgctggtgcg
      661 ccgggagtac atgaccgcca tcgggccggt cgacctgcta tgccgcgacg agcagggccg
      721 ttcggtggcc gtcgagatca agcgacgcgg cgagatcgat ggagtcgaac agctcacccg
      781 ctacctcgac ctgctcaacc gcgacaccgt gctggcgccc gtcagcggta ttttcgcggc
      841 ccagcagatc aagccgcagg ctcgaaccct ggccaccgat cgcgggatcc gttgtgtgac
      901 tttggattac gacgcgatgc gcggtatgga cagcgacgaa ttccggctgt tctgacagac
      961 tggtgacatg cctaggcgcc ggaatccgcc tcgcaggcaa cagcccctag cgccgttgcc
     1021 tgcggcccgc cggatcgagg ccggacctga cggctacgac tacgaggtaa ggccgatccc
     1081 cgcttcacgt gccaccaaga tctaccgctg tcctgggtgt gaccacgaaa tcgtcattgg
     1141 cacagcgcat ttagtagtgt ggccaactga tttgggggag cgggcggtcg acgagcgtcg
     1201 ccattggcat acgccgtgct ggacgcacag agctacacgc agcccgacgc gcagatggtc
     1261 ttaacggatc agtgctcggc ggccggctcg acgagctcga ccagtacccc gccggcgtcc
     1321 ttggggtgaa tgaagttgat ccgggaatcg gcggtgccgc gccgcggcgc ctcgtagagc
     1381 agccggacgc cctgctcgcg cagccgctcg gtcagcgcgt cgatatcgct gacgcggtag
     1441 gccagctgct gcaggccggg gccgcgcttg tcgaggaact tcgcgatcgt cgaggtctcg
     1501 tccagcgggg ccattagctg gagctgggcg ctgcccaccg gcgcgccccg caccgacagc
     1561 attgcctcgc ggacaccttg gtcctcgttg acctcttcgt gcaggacaat catgccgagg
     1621 tggtcgtgat accacttgat cgccgcgtcc agatcggcca cggcaatgcc gatgtgatcg
     1681 attgctgtca ccagggcggt ggccagcaac gggcgggcat cgacttgctc ggcggtcata
     1741 acgtaacggt aacctggcgg gaaagattgc gcctcgccgg ttggcgactt gagcggttca
     1801 gaagagcctt tggaggtagg aatgacgacg tctgtgatcg ttgctggagc ccgcacgccg
     1861 gtcggcaaat tgatgggttc gctgaaggat ttctcgggca gcgatctggg cgccatcgcc
     1921 atctccggtg cgctggagaa agccggtgtc ccggcgtcgc tggtggagta cgtgatcatg
     1981 ggccaggttc tgaccgccgg cgctgggcag atgccagcgc ggcaagccgc ggtcgccgcc
     2041 ggtatcggct gggatgtgcc ttcgctgacc atcaacaaga tgtgtttgtc cggcatagat
     2101 gccattgcac tggccgatca gctgatccgg gccggcgagt tcgatgtggt cgtggccggc
     2161 gggcaggagt cgatgaccaa ggcgccgcat cttttgatgg atagccgggc gggctacaaa
     2221 tatggcgacg tcacggtgct cgatcacatg gcctacgacg gcctgcacga cgtgttcacc
     2281 gaccagccga tgggcgcgct gaccgagcac cgcaacgatg tcgatcagtt cacccgcgcg
     2341 gagcaggacg agttcgctgc gcagtcgcac caaaaggccg ctgcggcatg gaaggacggc
     2401 gtctacgccg acgaggtggt gccggtgaag attccgcagc gcaagggtga tccgatcgag
     2461 ttcaccgagg acgagggcat ccgcgccaac accacggccg aatcgctggc cggactgaag
     2521 cccgcgttcc gcaaggacgg caccatcacc gccgggtccg cctcgcagat ctccgacggc
     2581 gcggccgcgg tcgtggtgat gaacaaggcc aaggcgcagg agctgggcct gacgtggctc
     2641 gccgagatcg gtgcccacgg cgttgtcgcg ggtccggact ccactctgca gtcgcagccg
     2701 gcgaacgcca tcaagaaagc cattgcccgc gagggcattt cggtcgacca gctcgatgtc
     2761 atcgagatca acgaggcctt ctcggcggtc gcgctggcgt cgatgaagga actcggcgtc
     2821 gatccgaaga aggtcaacgt gaacggcgga gccatcgcga tcggccatcc gatcggcatg
     2881 tccggagcac ggatcaccct tcacgccgcc cttgagctgg cacgacgtgg ttccggctac
     2941 gcggtggccg cgttgtgcgg ggccggcggt cagggcgacg ccctgatcct gcgcgcagga
     3001 tagccgtcta cgacgcaatg tagtagctcg ggcgtttttc gtgcacaatg gcgcccatga
     3061 cacccgcatt tgaccttcgg tccgctgccg gctggttccg attgatcgcc ctggcggagg
     3121 ccgccagctg ggtcgggctg ctgatcggca tgtacctcaa gtacctgggc acgccgcgca
     3181 ccgagatcgg cgtcaaggtg ttcggcatgg cccacgggct gatcttcatc gccttcgtcg
     3241 tggtcggtct gctcgtcgga atcgcgttca aatgggcggc cggcacatgg ttgctggcgt
     3301 tgctggcgag catcgtgcca ctgtgcagtg tgatcttcct catatgggcc gataggacgg
     3361 gccgaatggg acttacggaa gccacttcgg ccgtcgcgca accggggcgg cgcgcacccg
     3421 aaacgacgtg acagacttgg ggtcgtgagt cgcccccgac cccctgtcgc ggcctccatg
     3481 gccggcgcgg ttgatctgtc cgccctcaaa caacgcgcgg cacccgccgg cgaaggcgcc
     3541 gcggcctcgc cgtctggagt ggagataacc gaggccaacc tcgaagccga ggtgctggtc
     3601 cggtccagtc agatgccggt cgtcgtgctg ctgtggtcgc cccgcagtga ggcgagcgcg
     3661 caactcggcg atgtgctggg ccaactcgcg gccaccgacg gtggcaagtg gtcgctggcc
     3721 accgtgaacg tcgacgcggt accgcgggtc gcgcagatgt tcggtgtgca ggccgtgccg
     3781 acggtcgtgg cgctcgctgc gggccagccg atttcgagct tccaaggtgt acagccgccg
     3841 gagcagctgc gccggtgggt cgactcgctg ctgaacgcga cggcgggcaa gctcggcggc
     3901 gcggcagatg gcgatgagcc tgagcaggtc gaccccgaac tcgcgaaggc ccgcgaacac
     3961 ctcgacggcg gggacttcga cgcggccctg aaggcgtatc aagcgctgct cgacgccaac
     4021 ccgaaccatg cggaagccaa gggcgcggtg cggcagattg ggttcctaca gcgcgcgacc
     4081 tcccgcgcgc ccggcgcgat cgtcgccgcc gacgccaaac ccgatgacat tgagttggct
     4141 ttcgccgcag ccgatgtcga gatcttgcag cagagcgtcg cttcggcgtt cgatcggctc
     4201 atcgcactga tcaagcggac cgccggtgac gagcgcacca aggtccgcac ccggctgatc
     4261 gagttgttcg atctgttcga ccccgccgac cccgaggtga tcgcgggccg gcgcaacctc
     4321 gccaatgcgc tctattaagc atggcgagca gacgcaaaag tgccctattt gggccgaaat
     4381 agggcagttt tgcgtctgct cggcgtaatc aggccggttc gaaccacagc gcggccagcg
     4441 gtggtagcac catcaccgct gatgccgggc ggccgtgcca tggctcgtcg gtggcttgca
     4501 ctgcgccgta gttgccgata ccggtgccgt tgtagaggtc ggcgtcggtg ttgagcacct
     4561 cgcgccacgt gcccgcgtgc ggcaagccga gttggtagcg gctgtgttcg gagccggaga
     4621 agttgaacac gcatgccatc accgacccgt cgtcgccgta gcgcaggaag ctcagcacat
     4681 tgttggccga gtcgttggcg tcgatccac
//
LOCUS       DASRJA010000172         9157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1284900,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000172 DASRJA010000000
VERSION     DASRJA010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9157
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284900"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VFC01_08015"
     CDS             <1..935
                     /locus_tag="VFC01_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN79616.1"
                     /translation="NPADRIAELDRQGVAEALNYPTLASLIEHATADDPELTLAIIHA
                     LNQWMLEHWTYNYENRVFSTPIINLSEVDGAQRELEYLLNNGVKVALVKPGPVNGIHG
                     WRSPALPEFDPFWRDVEAAGLPIVLHASYPPLDDYVAKWEPPHTQNFMAQSAFRWMVL
                     GHREISDMITSLICHGTLTRFPKLRIASVENGSSWIYPLFGDFEDLHKKMPQNFPEHP
                     HDVFRRNIWVSPFWEGCVADVVNTVGWDKVLFGSDYPHPEGLAEPKGFWRYAEGMDER
                     RTYDFMGDNARRFMGLPIANPDPAAVKPPVLATF"
     gene            complement(999..2195)
                     /locus_tag="VFC01_08020"
     CDS             complement(999..2195)
                     /locus_tag="VFC01_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN79617.1"
                     /translation="MDFSRVQLSEDDQAFLEEARDFLRTHVTDDVIRRDRETGDNFDE
                     GVHLALGAAGYLTQDWKPESAGGFNRVRRRIWELEKRRAHVPWVTSGTTAMIARSVEK
                     FGSAELKAEVMPGVFSGHIRLCLGYTEPEGGSDVATCKTRAVRDGDGWIINGSKMFTT
                     GAHNCQYVFLITNTDPDAPKHKSLTMFLVPLASPGIEIQGVRTVDGDRTNIVFYSDVR
                     VDDKYRLGEVNDGWTVVREPLNVEHGAVDAAADGLQDVSIMMHQAGFMAAAVDTAAAK
                     VAEADPNGRRLIDDQSVSYRLGRSVARMEAALSASTIFGRVALAQTMRDISPDLMDIL
                     GTASTLPVGTDGAADDGAAEYAYRFAPLVGIYGGTLEVFRNMIAQYVLGLGKPAYAPP
                     AQKKAS"
     gene            complement(2196..3248)
                     /locus_tag="VFC01_08025"
     CDS             complement(2196..3248)
                     /locus_tag="VFC01_08025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN79618.1"
                     /translation="MDRYELRRLDYSLTEDHQALQAAYKDFFKTHCSIETVRAAEEPG
                     FDKSLWERLCAMGATTMALPESVGGDGATLVDLTLVAEEIGRSLAPVPWIDHICAARL
                     LARLDALQPDVVNGKQLAAFDPQHDNVSGVRLIPTGSIADQIVVRDGEDIVRLTFGTR
                     PAKVDNIGKLPMAWVDPASADSRTVLASGAQALAEYQRALDEWRVLIAAALVGLVEES
                     LTIAAEFAKTRYTLGVPISTLQGISHPLANIAITVQGGRNLARRAAWFLDNEPDERPE
                     LAPSSFVFMAEEASKAATMCVHVQGGLGVSAEAAATAYLVRARGWALAGGDPGASAKY
                     IAEIVAARESGSGKGA"
     gene            complement(3248..4090)
                     /locus_tag="VFC01_08030"
     CDS             complement(3248..4090)
                     /locus_tag="VFC01_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN79619.1"
                     /translation="MTHRAIDGLVNVHFGETEKQPTWMLKVRDDYFKGPESMFAPVDL
                     TELLDEMDEQGVAKAILMDNLAKPSVTARKFVEAKPDRFALAMGGVNLLRPMPSLREL
                     AAVVNDLPVAYAVVGPSFWGDGQYPPSDAVYYPLYTKCAELGLPLCINTGIPGPPIPG
                     EVQNPIHLDRVCVRFPELKLCMIHGADPWWDIAIRLMIKYKNLRLMTSAWSPKRLPDS
                     LLHYMRTRGRDKVIYASDWPVLKMRRVVPEALVLDLPADVLDNYLYNNANEFFFGDRA
                     REEH"
     gene            complement(4087..5700)
                     /locus_tag="VFC01_08035"
     CDS             complement(4087..5700)
                     /locus_tag="VFC01_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HZN79620.1"
                     /translation="MSEWTIGAVLDAIAEAVPDRLMTVCGGRSSTFGESADRTRRLAN
                     FLASSGFGVHREREELENWECGQDRVALIMHNDLYPDMVIGCLKARTVPANVNYNYTP
                     REVAELLDYLKPRAIIYHRSFGAKFADVLTAGTAELIISVDDGTEDAELPAAVKLEDA
                     LAQGHTDQDITPSPDDLMMVCTGGTTGRPKGVMWRQSDTYVVSMNGADHESADEIREK
                     VRHGGQPWFAVSPLMHAAGMWTAFAALLNGLPVVLYDRTKFDPPAVLETAERHKVGMM
                     TMVGDAYAGPLVEELRRGSYDLSSMFAIGTGGAATNPKYQQALLELLPQITLINGYGS
                     SETGNVGFGRSQRGDQKDTFALREGGLVVSDDYRRFLQPGEDEIGYVARAGRIPLGYF
                     DDPVATRKTFPVVDGQRVVISGDRGSLAADGTMRLFGRDSLVINTGGEKVFVEEVEEV
                     LRAHPAVADALVVGRPSERWGQEVVALVALRDGGEADHGVLHEQCTSQLARFKAPKEF
                     IFVEQVRRLGNGKADYRWAKSAATQKANV"
     gene            complement(5713..6915)
                     /locus_tag="VFC01_08040"
     CDS             complement(5713..6915)
                     /locus_tag="VFC01_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN79621.1"
                     /translation="MTLDYKAIDVDNHYYEPLDALTRHLPKEFKRRGVQMVQDGKRTW
                     AVMGERVNHFIPNPTFDPIIEPGCLDLLFRGEIPEGVDPASLMKVDRLADHPEYQNRD
                     ARVKILDKQNLETAFMLPTFACGVEEALKNDIEATMASMHAFNLWLDEDWGFDRPDHR
                     FISAPIISLADPDKAVEEVEFVLGRGAKMVLVRPAPVPGVVKPRSLGDPLHDPVWARL
                     AEAGVPVGFHLSDSGYLAIAALWGGKATFEGFGKKDPLDQVLLDDRAIHDTMASMIVH
                     QVFTRHPKLKVASIENGSYFVYRLIKRLKKAANTAPYHFKEDPVEQLRNHVWIAPYYE
                     DDVKLLAETIGVDKILFGSDWPHGEGLADPMSFTADIPQFPEFSAEDTRKVMRDNALD
                     LLGVKVSA"
     gene            complement(6912..7763)
                     /locus_tag="VFC01_08045"
     CDS             complement(6912..7763)
                     /locus_tag="VFC01_08045"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZN79622.1"
                     /translation="MSVPDRVAEPPVTSDPVLYAIRDTGVAVLTLNRPERMNGWGGGL
                     ATTFYSLLDDAEADPDVRAIVVTGSGRAFCAGADMGDLTTISNATVDAAGDTDVSKLV
                     GARHPHHVMTMRKPVIAAINGACAGMGLTMALACDVRFAAAGAKFTTSFARRGLIAEY
                     GISWILPRLVGQAVALDLLLSGRAFYADEAAELGLVKEVVPPEELLPRAIAYAQDIAA
                     NCAPSSLAVIKQQVYADSMSDVFEASDRSEKLMHESMQRPDFIEGITSFFEKRPPNFP
                     PLKGQDQ"
     gene            complement(7866..8873)
                     /locus_tag="VFC01_08050"
     CDS             complement(7866..8873)
                     /locus_tag="VFC01_08050"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZN79623.1"
                     /translation="MATAIQARDWARATLRGIGNSLYTPFSGVDGDDIDWDAYRTLVR
                     YCVGELRHPMLWCTSGIAEFWSLTLDERKRLLEVAIEEGRAADPDVVVQACTAAMSAK
                     DCLELTRHAQQAGADIVYIQTPMMEAHGGEGVLKFFRYVAERTDIALGMFNSPSSGYV
                     LTPAESARIYDEIPAVCATKEGAFRPASSRMLHELAPDLVIWECDLTVYRAGWLRAGI
                     VGPAQLGTAGYLYETPQQPIISEYWDLVWNDKLIEAMDFAEKSGLDQFGVDMRSWFTC
                     YPGRPDYFTHWGGAFKYAASLLGLPIGDYPHSRPPQAELPDEGRTQIRTAYQRFGLIA
                     Q"
     gene            complement(8884..>9157)
                     /locus_tag="VFC01_08055"
     CDS             complement(8884..>9157)
                     /locus_tag="VFC01_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN79624.1"
                     /translation="LAPDEIMVNVVSPGSILSESLAGWADSVGIDGSDPYAVMAGIDK
                     HFGHPAHLPRAGKPDEIGPVVAFLVSRRNSYMTGANINVDGGSDFT"
BASE COUNT         1596 a         3046 c         2840 g         1675 t
ORIGIN      
        1 gcaaccctgc cgaccgcatc gccgaactgg accgccaggg cgtcgccgag gcactgaact
       61 acccgacgct ggccagcctc atcgagcacg ctaccgccga cgaccccgag ctgacgctgg
      121 cgatcatcca cgccctcaac cagtggatgc tcgagcactg gacctacaac tacgagaatc
      181 gcgtgttctc cacgccgatc atcaacctgt cggaagtcga cggcgcccag cgggaactcg
      241 agtacctgct gaacaacggc gtcaaggtcg cactggtcaa gcccggcccg gtcaatggca
      301 tccacggctg gcgctccccc gcactaccgg agttcgaccc gttctggcgt gacgtcgagg
      361 ccgcgggcct gccgatcgtc ttgcacgcca gctatccgcc gctcgacgac tacgtcgcca
      421 agtgggagcc gccgcacacg cagaatttca tggcgcagag cgcgtttcgc tggatggtgc
      481 tgggccaccg cgaaatttcc gacatgatca ccagcctgat ctgccacggc accctgaccc
      541 gcttccccaa gttgcgcata gccagcgtcg agaacggcag ctcgtggatc tacccgttgt
      601 tcggtgactt cgaagacctg cacaagaaga tgccgcagaa cttcccggag catccgcacg
      661 acgtgttccg gcgcaacatc tgggtcagcc cgttctggga gggctgcgtg gccgacgtcg
      721 tcaacaccgt cggctgggac aaggtgttgt tcggttcgga ctacccgcac cccgaaggtc
      781 tcgccgagcc aaagggtttc tggcgctacg ccgaaggcat ggacgagcgg cggacctacg
      841 acttcatggg cgacaacgcc cgccggttca tgggtctgcc gatcgcgaac ccggacccgg
      901 ccgcggtcaa gccacccgtg ttggccacct tctaacgccg agcagacgca aaagtgccca
      961 taatccacgg aaaataggca tgtttgcgtc tgctcgcgct aggaagcctt cttctgcgcc
     1021 ggcggcgcgt atgcgggctt gcccagcccg agcacgtact gcgcgatcat gttgcggaac
     1081 acctccagcg tgccgccata gatgccgacc agcggagcga accggtaagc gtattctgcc
     1141 gcgccgtcat cggccgcgcc gtcggtaccg acgggcagcg tcgacgcggt gcccaggatg
     1201 tccatcaggt ccggcgagat gtcacgcatc gtctgcgcca gtgccaccct gccgaagatc
     1261 gtcgaggcgc tcagcgccgc ttccatccga gcaacgctgc gtcccaaccg atatgacacc
     1321 gactgatcgt cgatcaaccg gcggccgttg ggatccgcct cggcgacctt cgccgccgcc
     1381 gtgtcgacag ccgccgccat gaaaccagcc tgatgcatca tgatcgacac atcctgcaga
     1441 ccgtccgctg ccgcgtccac ggcgccgtgc tcgacgttga gcggttcacg caccaccgtc
     1501 cagccatcgt tgacctcgcc gagccggtat ttgtcgtcga cgcgaacgtc gctgtagaag
     1561 acgatgttgg tgcggtcgcc gtccacggtg cggacgccct ggatctcgat tcccggcgaa
     1621 gccagcggga ccaggaacat ggtcaggctc ttgtgcttcg gcgcgtccgg atcggtgttg
     1681 gtgatcaaga agacgtactg gcagttgtgc gcgccggtag tgaacatctt ggagccgttg
     1741 atgatccagc cgtcaccgtc gcgcaccgcg cgggtcttgc acgtggccac atcggagccg
     1801 ccctcaggtt cggtgtagcc caggcacagc cggatatgac cgctgaacac gcccggcatc
     1861 acttcggcct tgagttccgc tgagccgaac ttctccaccg accgcgcgat catcgcggtg
     1921 gtacccgagg tcacccacgg cacgtgcgcg cgtcgcttct ccagttccca gatccgccgc
     1981 cgcacccggt tgaagccgcc tgcggattcc ggcttccagt cttgcgtcag gtatcccgcg
     2041 gcgcccaaag cgagatgcac gccttcgtcg aagttgtcgc cggtttcgcg atcgcggcgg
     2101 atgacgtcgt cggtgacgtg cgtacgcagg aagtcgcgcg cctcctcgag gaatgcctga
     2161 tcatcctcgg acagctgaac ccgtgagaaa tccacttatg cgccctttcc tgaaccactt
     2221 tcgcgggccg cgacgatttc ggcgatgtac ttggcgctcg cacccgggtc accaccggct
     2281 agcgcccagc cgcgagcgcg gaccagatag gccgtcgcgg ccgcctctgc ggagacaccc
     2341 agaccgcctt ggacgtgcac acacatcgtt gcggccttcg atgcttcctc ggccatgaac
     2401 acaaacgacg atggcgccaa ctccggccgt tcgtcgggct cgttgtccaa gaaccacgct
     2461 gcgcggcggg ccagattgcg gccgccctgc acggtgatcg cgatgttggc cagaggatgc
     2521 gagatgccct gcaatgtcga gatcggcaca cccaacgtgt agcgggtctt ggcgaactcc
     2581 gcggcgatcg tcagtgactc ctcgaccagc cccaccaatg cggcagcgat cagaactcgc
     2641 cattcgtcca gcgcgcgttg gtattccgcc agtgcctgag ccccgctggc caaaaccgtg
     2701 cgactgtcgg ccgacgctgg gtcgacccac gccatcggca gcttgccgat gttgtcgacc
     2761 ttcgccggac gggtaccgaa cgtcagtcgc acaatgtcct cgccgtcacg cacgacgatc
     2821 tggtcggcga tcgatccggt cgggatcagc ctgacgccgg acacattgtc atgctgcgga
     2881 tcgaacgcgg ccagctgctt gccgttgacg acgtctggct ggagcgcgtc caagcgggcg
     2941 agcagccggg cggcgcagat gtggtcgatc catggcacgg gtgcaagcga gcgtccgatc
     3001 tcctcggcca ccagcgtcaa gtcgaccagt gtcgccccgt ctcccccaac ggattccggt
     3061 aacgccatcg tcgtcgcacc catcgcgcac aaccgctccc acaggctctt gtcgaaccct
     3121 ggctcctccg cggcccgcac cgtttcgatc gaacaatgcg tcttgaaaaa gtccttgtaa
     3181 gcagcctgta gtgcctgatg gtcctccgtc aggctgtagt cgagtctgcg aagttcgtag
     3241 cgatccatca gtgctcctcg cgtgcgcggt ccccgaaaaa gaattcgttg gcgttgttgt
     3301 agagatagtt gtcgagtacg tcggcgggca gatccagcac aagtgcctcg gggacgacgc
     3361 ggcgcatctt cagcaccggc cagtccgacg cgtagatcac cttgtcccgg cctcgcgtcc
     3421 gcatgtagtg caagaggctg tcggggagtc gcttgggtga ccaggccgac gtcatgaggc
     3481 gcagattctt gtacttgatc atcagccgga tcgcgatgtc ccaccagggg tcggcgccgt
     3541 gaatcatgca gagcttgagt tcggggaagc gtacgcaaac ccggtcgagg tggattgggt
     3601 tctgtacttc accggggatc ggcggccctg ggatgccggt gttgatgcac agcggcagcc
     3661 cgagttcggc gcatttggtg tacagcgggt agtacacggc gtcgctgggc gggtattggc
     3721 catcgcccca aaagcttggg ccgacaacgg catacgccac cggcaggtcg ttgaccactg
     3781 cggccaattc ccgcagtgag ggcatcggcc gcagcaagtt gacgccaccc atcgccagag
     3841 cgaaccggtc cggcttcgct tcgacgaact tgcgcgcggt caccgatggt ttggcgagat
     3901 tgtccatcag gatcgccttc gccacgccct gctcatccat ctcgtcgagc agctccgtca
     3961 ggtccaccgg cgcgaacatc gactccggac ccttgaagta gtcgtcgcgc accttgagca
     4021 tccaggtcgg ctgcttctcc gtttcgccga agtgcacgtt gaccaaaccg tcgatcgctc
     4081 tgtgagtcat acgtttgcct tttgtgtcgc agcgcttttc gcccatcgat agtccgcctt
     4141 gccattgccc agccggcgga cctgctcgac gaagatgaac tccttcggtg ctttgaatcg
     4201 agcgagctgt gacgtgcact gctcgtgcag caccccatga tcggcctcgc caccgtcgcg
     4261 caatgccacc agtgcgacga cttcctgacc ccatcgttca ctcggcctgc ctaccaccaa
     4321 cgcgtcggcg acggccgggt gcgcgcgaag cacctcttcg acttcctcca cgaacacctt
     4381 ctcgccgccg gtgttgatga ccaaggagtc gcgaccgaac agccgcatcg ttccgtcggc
     4441 cgccagcgag ccgcggtccc cggaaatcac cacacgctgt ccgtcgacga ccggaaatgt
     4501 cttgcgagtg gcgacgggat cgtcgaaata gcccagcggg atgcggcctg cgcgggcgac
     4561 atatccgatc tcgtcctcgc cgggctgcaa gaaccggcga tagtcatcgg agaccaccaa
     4621 accgccctcg cgcagcgcga aggtgtcctt ctggtcccca cgctgactgc gaccgaagcc
     4681 cacgttcccg gtctccgacg agccgtaccc gttgatcagc gtgatctgcg gcagcaattc
     4741 cagcagcgcc tgttggtatt tcgggttcgt ggccgctccc ccggtgccga tcgcgaacat
     4801 cgacgacaga tcgtaggaac cgcgtcgcag ctcctcgacc aacggcccgg cgtaggcgtc
     4861 gccgaccatc gtcatcattc cgaccttgtg gcgctccgca gtctcgagca cggcgggcgg
     4921 gtcgaacttc gtccggtcgt agagcacgac gggcaggccg ttgaggaggg ccgcgaaggc
     4981 cgtccacatg cccgccgcgt gcatcagcgg cgacacggca aaccacggct gcccgccgtg
     5041 gcgcaccttt tcgcgtatct cgtcggcgga ttcgtggtcg gcgccgttca tcgatacgac
     5101 gtaggtgtcg ctctgccgcc acatgacacc cttgggcctg ccggtggtac cgccggtgca
     5161 gaccatcatc agatcgtccg gagagggcgt gatgtcttga tccgtatgtc cttgggccag
     5221 tgcatcttcc agcttcaccg ctgcgggtag ctctgcatcc tcggtgccgt cgtcaaccga
     5281 gatgatcagt tcggccgtgc cggcggtcaa tacgtcggcg aacttcgcgc cgaatgaccg
     5341 gtggtagatg atggcccgcg gcttgaggta gtccagcagt tcggcgacct cgcgcggcgt
     5401 gtagttgtag ttgacgttgg ccgggaccgt tcgggccttc agacagccga tcaccatgtc
     5461 ggggtacaga tcgttgtgca tgatgagcgc aacccgatcc tgaccgcact cccagttctc
     5521 gagctcctcg cgctcacggt ggacaccaaa cccgctgctg gcaagaaaat tggccagccg
     5581 gcgggttcga tccgccgatt cgccgaacgt gctgctgcgt ccgccgcaga ccgtcatcag
     5641 gcgatccgga acggcctcag caatcgcgtc cagaaccgcg ccgatcgtcc attcgctcat
     5701 gtgcttgggc tgctacgccg acaccttgac gccgagcagg tcaagagcgt tgtcgcgcat
     5761 aaccttccgg gtgtcttccg cgctgaactc agggaactgg gggatgtctg cggtgaagga
     5821 catgggatcc gcgagtccct cgccgtgcgg ccagtccgag ccgaacagaa tcttgtccac
     5881 gccaatggtt tcggcgagca gcttcacgtc gtcctcgtag tacggcgcga tccagacgtg
     5941 gttgcgcagc tgctcgaccg ggtcctcctt gaagtggtag ggcgcggtgt tggcggcctt
     6001 cttcaggcgt ttgatcaacc ggtagacgaa gtacgagccg ttttcgatgc tggcgacctt
     6061 cagcttggga tggcgggtga acacctggtg cacgatcatc gaggccatcg tgtcgtggat
     6121 ggcccggtcg tccagcagca cctgatcgag cggatccttc ttgccgaatc cctcgaacgt
     6181 cgccttgccg ccccacaacg ccgcgatcgc gagatagcca ctgtccgaca ggtggaagcc
     6241 caccgggacc ccggcctccg ccagccgcgc ccacaccgga tcgtgcagcg ggtcgcccag
     6301 cgagcgcggc ttgaccacac ccggcaccgg tgccggtcgg accaggacca tcttcgcgcc
     6361 gcgaccgagg acgaactcga cttcctcgac ggccttgtcc ggatcggcca gcgaaatgat
     6421 cggcgccgaa atgaaccggt ggtcgggacg atcgaagccc cagtcctcgt cgagccagag
     6481 gttgaacgcg tgcatcgatg ccatcgtcgc ctcgatgtcg ttcttgagcg cctcctcgac
     6541 gccgcacgcg aacgtcggca gcatgaacgc cgtttcgagg ttctgcttgt cgaggatctt
     6601 cactcgggca tcgcggttct ggtactcggg gtgatctgcc aaccggtcga ccttcatcag
     6661 cgacgcgggg tcgacgcctt ccggaatctc gccgcggaac aacagatcca ggcagcccgg
     6721 ctcgatgatg gggtcgaatg tcgggttcgg gatgaaatgg ttgacgcgtt cacccatcac
     6781 cgcccaggtg cgcttgccgt cctgaaccat ctgcacgcca cggcgcttga actccttggg
     6841 caggtgccgg gtcagcgcgt cgagtggctc gtagtagtgg ttgtcgacgt cgatcgcctt
     6901 gtagtccagt gtcactggtc ctgtcctttc aacggcggga agttgggcgg acgcttttcg
     6961 aaaaagctcg tgatgccctc gatgaagtcg ggccgctgca tggactcgtg catgagcttc
     7021 tccgagcggt cgctggcctc gaagacatcg gacatgctgt ctgcgtacac ctgttgcttg
     7081 atcaccgcta gagaactggg tgcacagttg gcggcgatgt cctgggcgta ggcgatcgcc
     7141 cgcggcaaca gctcttcggg cggcacgact tccttgacga gacccaactc ggccgcctcg
     7201 tcggcgtaga aggcccgacc gctgagcagc aggtcgagcg cgacagcctg gccgacgagc
     7261 cggggaagaa tccaggagat cccgtactcc gcgatcagcc cgcgtcgcgc gaacgacgtc
     7321 gtgaacttgg cgcccgcggc ggcaaagcgg acatcgcagg ccaacgccat cgtcaggccc
     7381 attcctgcgc aggcgccgtt gatcgcggcg atgacgggct tgcgcatggt cattacgtgg
     7441 tgcggatgcc gagcgccgac gagcttgctg acatcggtgt ctccggcggc gtcgacggtg
     7501 gcattgctga tcgtggtcag gtcgcccatg tccgcgcccg cgcagaacgc gcggccgctg
     7561 ccggtgacca cgatggcccg gacgtcggga tcggcctccg cgtcgtcgag cagtgagtaa
     7621 aacgtggtcg cgagaccgcc gccccacccg ttcatccgct cgggccgatt gagggtcaat
     7681 accgcgacgc cggtgtcgcg gatcgcgtag aggaccggat ccgacgtcac cgggggttcc
     7741 gcgacgcggt cagggacgct cacgcagcag acctcctcgg gagtggcatc gagctcgatg
     7801 tattcccata ctgtacacct atcggtagtg ccttccgcaa gcggccgagg ggcgccccaa
     7861 cggccctatt gcgcgatcaa tccgaagcgc tgatacgccg ttcgaatctg tgtccgcccc
     7921 tcgtcgggca gctccgcttg tggaggcctc gaatgcggat agtccccgat cggcaggccg
     7981 agcagcgacg ccgcgtattt gaatgcgccg ccccagtggg tgaagtagtc gggacggccg
     8041 ggatagcagg taaaccacga gcgcatgtcg acaccgaact ggtcgagccc ggacttctcg
     8101 gcgaagtcca tcgcctcgat cagcttgtcg ttccacacca ggtcccaata ctcggaaatt
     8161 attggctgtt gcggagtttc gtagagatag ccggccgtgc cgagttgtgc gggcccgacg
     8221 atgccggcac gcagccaccc tgcgcggtag acagtcaagt cgcattccca gatcaccagg
     8281 tcgggcgcga gctcgtgcaa catccggctg ctcgccggcc ggaacgcacc ctccttggtg
     8341 gcgcagacgg ctgggatttc gtcgtagatg cgcgcactct cagcgggagt cagcacatat
     8401 ccggacgacg gtgaattgaa catgccaagc gcgatgtcgg tgcgctcggc gacgtagcgg
     8461 aagaacttca gtacgccttc gccgccgtgg gcctccatca tcggagtctg gatgtagacg
     8521 atgtccgccc cggcctgctg ggcgtgccgc gtcaattcca ggcaatcctt ggccgacatt
     8581 gcagccgtgc aggcctgcac gacgacatcg gggtcggccg cacgcccttc ctcaatcgcg
     8641 acctccagca agcgctttcg ctcgtcgagc gtcaaagacc agaactccgc gatgccgctg
     8701 gtgcaccaca gcatcgggtg tcgcagctcg ccgacgcagt agcgcaccag cgttcggtaa
     8761 gcatcccagt cgatgtcgtc gccgtcgaca ccactgaagg gtgtgtagag actgttgccg
     8821 atgccgcgca gcgttgcacg cgcccaatca cgagcttgga tggcagtcgc cataattcac
     8881 tccctaggtg aaatcactac cgccgtcgac gttgatgttg gcgccggtca tgtaggagtt
     8941 gcgccgcgag accaaaaatg cgacgacggg gccgatctcg tcgggctttc ccgcgcgggg
     9001 cagatgtgca ggatgcccga aatgcttgtc tatgcctgcc attacggcgt aggggtcgct
     9061 cccgtcgatg ccaacggagt ctgcccagcc cgccagcgac tccgaaagga tgctgcccgg
     9121 cgagacgacg ttcaccatga tctcgtcggg ggccagc
//
LOCUS       DASRJA010000173         6446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_721873,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000173 DASRJA010000000
VERSION     DASRJA010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721873"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="VFC01_08060"
     CDS             complement(<1..1488)
                     /locus_tag="VFC01_08060"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016886844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HZN79625.1"
                     /translation="MDNGQNAKQDQLDGSRVSRETGYLTTQQAVRVDHTDDALTVGER
                     GPTLLEDFHAREKITHFDHERIPERVVHARGAGAYGYFEPYNDSLAEYTVAKFLTTPG
                     KQTPVFVRFSTVAGSRGSADTVRDVRGFATKFYTDQGNYDLVGNNFPVFFIQDGIKFP
                     DFVHAVKPEPHNEIPQAASAHDTLWDFVSLQPETLHAIMWLMSDRALPRSYRMMQGFG
                     VHTFRFVNARGDGTFVKFHWKPKLGVHSLVWDECQKVAGKDPDFNRRDLWDAIEAGDF
                     PEWELGVQLVPETDEFNFAFDLLDATKLIPEEQVPVQPVGKMVLNRNPDNFFAETEQV
                     AFHTANVVPGIDFTNDPLLQFRNFSYLDTQLIRLGGPNFAQLPVNRPLADVRNNQQDG
                     YGQHAIPQGRSSYFKNTLGGGCPALADEDVFRHYTEKVDGNKIRQRAKSFEDHYSQAR
                     MFWKSMSPVEAKHIVAAYAFELGKVEVPEIRTRVVDQLNLVDHDLA"
     gene            complement(1529..2755)
                     /locus_tag="VFC01_08065"
     CDS             complement(1529..2755)
                     /locus_tag="VFC01_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HZN79626.1"
                     /translation="MSEQVDEPPPWPAELTLTAGPPANGGSCVARHEGRVVFVRYALP
                     GETVKVRVVGDHGSYWHADVVEVIEPSPNRIDSLCPIAGVDGAGCCDLAFADPDATRR
                     LKGTVVANQLARLGGYQWCDEDSAVAEPVGDGSATGWRTRVRLDTSAEGRAGFHRYHS
                     SQLVTELNCAQLPAGMLDGLNEWRWPPGAQLHVAIDDDEVRHVVQSGPRTGRKTTTVV
                     AEGHYEAVQRVGDRIWRVPVTAFWQAHRDAARIYSELVASWAQLSAGQTAWDLYGGAG
                     LFAAALAEGVGERGKVLTVDTSRGASRSARTALADLGWVSVVTDSVRRALAAQTERAD
                     VAVLDPPRSGAGREVIDLLAAAEVPRVIHIGCEAASFARDIGLYLGHGYAVEDLRVFD
                     SFPLTHHVECVAALTR"
     gene            complement(2752..4746)
                     /locus_tag="VFC01_08070"
     CDS             complement(2752..4746)
                     /locus_tag="VFC01_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN79627.1"
                     /translation="MSKLSTAARRLVLGRPFRSDKLAHTLLPKRIALPVFASDALSSV
                     AYAPEEIFLVLSVAGLAAFSMAPWIGLAVGAVMLVVIASYRQNVHAYPSGGGDYEVVT
                     TNLGHTAGLTVASALLVDYVLTVAVSMASAMANIGSAVPLINHHKVLFAVIAILILAS
                     LNLRGIRESGTAFAVPTYAFMIGMYIMLAWGLFQIYVLGNPLRAESAGFEVHSEHGEV
                     LGFAMVFLVARAFSSGCAALTGVEAISNGVPAFRKPKSRNAATTLLMLGVIAVSLFMG
                     IIMLAKETGVKMAENPEQLVGAPPNYHQKTLIAQLADAVFHDFPLGLYLIAGVTALIL
                     VLAANTAFNGFPVLGSILAQDRYLPRQLHTRGDRLAFSNGILFLAFAAIAFVVAFRAE
                     VTALIQLYIVGVFVSFTLSQIGMVRHWTRLLRTETDSAVRRHMVRSRVINTIGFICTG
                     TVLIIVVVTKFVAGAWIAILAMGALFMIMKMIHKHYDTVAHELDRRAEEQGDDMVLPS
                     RNHAVVLVSKLHLPTKRALAYARATRPDVLEAITVSVDDAETRELVHRWEDSDISVPL
                     KVIASPYREITRPVLDYVKRVTKESPRTVVTVFIPEYVVGHWWEQVLHNQSALRLKGR
                     LLFEPNVMVTSVPWQLTSSERLKTLQPHAAPGDARRGFLD"
     gene            4833..5507
                     /locus_tag="VFC01_08075"
     CDS             4833..5507
                     /locus_tag="VFC01_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HZN79628.1"
                     /translation="MRVVVMGCGRVGASLSDSLARIGHDVAVIDRDSTAFHRLSPEFS
                     GERVLGMGFDRDVLLRAGIEEAGAFAAVSSGDNSNIISARVARETFGIERVVARIYDA
                     KRAAVYERLGIPTVATVPWTTDRLLNVLTRETETTKWRDPTGNVGVTEMALHEEWAGH
                     RITNLEAATGARVAFLIRFGAGLLPDPKTVIQAGDQVYMAAIAGHIAEALAIAALPPS
                     EDTEAQ"
     gene            5504..6169
                     /locus_tag="VFC01_08080"
     CDS             5504..6169
                     /locus_tag="VFC01_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HZN79629.1"
                     /translation="MKVAIAGAGAVGRSIARELVETHDVTLLERNPDHIDVDAIPAAH
                     WRLGDACELSLLESVKLEEFDVVIAATGDDKVNVVVSLLAKTEFAVPRVVARVNDPRN
                     EWLFDENWGVDVAVSTPRMLASLVEEAVAVGDLVRLMEFRKGQANLVEITLPDDTPWG
                     GKPVKRLDLPRDASLVTILRGPRVIVPEADEPLEGGDELLFVATTEVEDELQALLLNQ
                     QSR"
     gene            complement(6173..>6446)
                     /locus_tag="VFC01_08085"
     CDS             complement(6173..>6446)
                     /locus_tag="VFC01_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN79630.1"
                     /translation="QSGIGSAINNAVSRIAGLIAVAFTGVIIGGAMDFAGFRQGALVT
                     AALFAVAGVISAIGIRNAECDFDRVSPEDAACCHDRATPPPAYARR"
BASE COUNT         1132 a         2144 c         2111 g         1059 t
ORIGIN      
        1 cgccaggtcg tgatcgacca ggttgagttg gtcgacgacg cgggtacgga tctcggggac
       61 ctccaccttg cccagttcga acgcatacgc ggcgacgatg tgtttggcct ccaccggcga
      121 catgctcttc caaaacatcc gcgcctggct gtagtggtcc tcaaagctct tggcgcgctg
      181 gcggatcttg ttgccgtcga ccttctccgt gtagtgccgg aacacgtcct cgtcggccag
      241 tgccgggcag ccgccgccga gcgtgttctt gaaatagctt gaccgcccct gcggtattgc
      301 gtgctgtccg tagccgtcct gctggttgtt gcggacgtcg gccagcggcc ggttgacggg
      361 cagctgggcg aagttcggcc cacccagccg gatcagctgg gtatccaggt acgagaagtt
      421 gcggaactgc agcagcggat cgttggtgaa gtcgatcccg ggcaccacgt tggcggtgtg
      481 aaacgcgacc tgttcggtct cggcgaagaa gttgtcggga ttgcggttga gcaccatctt
      541 gcccaccggt tgaaccggaa cctgttcctc cggaatcaat ttcgtcgcat ccagcagatc
      601 gaatgcgaag ttgaattcgt cggtctcggg caccagttgc acgccgagct cccactccgg
      661 gaagtcgccg gcctcgatgg cgtcccacaa gtcgcggcgg ttgaagtccg ggtccttgcc
      721 cgcgaccttc tggcactcgt cccacaccaa cgaatgcaca cccagcttgg gcttccagtg
      781 aaacttgacg aacgtgccgt ccccgcgggc gttgacgaag cggaaggtgt gcacgccgaa
      841 gccctgcatc atccggtagc tgcgcggcag cgcccggtcc gacatcagcc acatgatcgc
      901 gtgcagcgtc tccggctgta gcgacacgaa atcccacagt gtgtcgtggg ccgacgcggc
      961 ctgcggaatc tcgttgtggg gctccggttt caccgcgtgc acgaagtccg ggaacttgat
     1021 gccgtcctga atgaagaaca ccggaaagtt gttgccgacg aggtcgtagt tgccctggtc
     1081 ggtgtagaac ttcgtcgcga agccgcgcac atcccgcacc gtgtcggccg agccgcgcga
     1141 gccggccacc gtggaaaacc gcacgaacac gggtgtctgc ttgcccggcg tggtcaagaa
     1201 cttcgcgacc gtgtactcgg cgagcgaatc gttgtacggc tcgaaatagc cgtaggcacc
     1261 cgcaccgcgg gcatgcacca cccgctccgg aatgcgctca tgatcgaaat gcgtgatctt
     1321 ctcgcgcgca tggaagtcct cgagcagggt gggaccgcgt tctcccacgg tcagcgcatc
     1381 gtcagtgtga tcgacgcgca ccgcctgctg ggtggtcagg tacccggtct ctcggctgac
     1441 gcgcgagccg tcgagctggt cttgctttgc gttctggccg ttgtccacca tggccgggcg
     1501 ggtacccacc aaatgcaacg acaaacggtc agcgggtcag cgccgcgaca cattcgacat
     1561 ggtgtgtcag cggaaacgag tcgaacaccc gcagatcctc gacggcatag ccgtgcccga
     1621 gatacagccc gatgtcgcgg gcaaaggacg ccgcctcgca gccgatgtgg atcacccggg
     1681 gcacctcggc ggcggccagc aggtcgatca cctcgcggcc ggccccggac cggggtggat
     1741 ccagcaccgc cacatcggct cgctcggtct gggccgcgag cgcccgtcgc accgagtcgg
     1801 tcaccaccga cacccagccg aggtcggcca gcgccgtgcg cgccgatcgc gacgcgccac
     1861 gcgacgtatc gacagtcagg accttgccgc gctccccgac cccctcagcc agcgccgcag
     1921 cgaaaagccc tgccccaccg tacaaatccc atgccgtctg accggcgctc agctgcgccc
     1981 agctggccac cagttcgctg tagatccggg ccgcgtcgcg gtgcgcctgc cagaacgcgg
     2041 tcaccggcac ccgccagatt cggtccccga cccgctgcac cgcctcgtag tgaccctcgg
     2101 cgacaacggt cgtcgtcttg cgtccggtcc gcggccctga ctgcacgacg tgccgcactt
     2161 cgtcgtcgtc gatcgccacg tgcagctgcg cgcccggtgg ccacctccac tcgttcaacc
     2221 cgtcgagcat gccggcggga agctgcgcgc agttcagctc ggtgacgagc tgcgagctgt
     2281 gatagcgatg gaagccggcc ctgccctcgg ccgacgtgtc gagccgcacc cgcgtccgcc
     2341 aaccggtggc gctgccgtct ccgaccggct cggcgacggc ggagtcctcg tcgcaccatt
     2401 ggtagccacc cagtcgggcc aattgattgg cgacgacggt gcccttcagt cggcgcgtgg
     2461 catcggggtc ggcgaacgcc agatcgcagc aaccggcgcc gtccacgccg gcgatcgggc
     2521 acagcgagtc gatgcggttg ggtgacggtt cgatgacctc gacaacgtct gcgtgccaat
     2581 aggatccgtg gtcgccgacg acccgcactt ttactgtctc accgggcaac gcataccgca
     2641 cgaacaccac ccggccttcg tggcgcgcaa cgcagctacc gccgttggcg ggcggcccgg
     2701 cggtcagcgt cagttccgcc ggccagggcg gcggctcgtc gacctgttcg ctcaatccaa
     2761 gaagcccctg cgtgcgtcac caggtgcagc gtgcggctgc agtgtcttga gtcgctccga
     2821 cgaagtcagt tgccacggaa ccgatgtcac catcacgttc ggctcgaaca gcaatcggcc
     2881 cttcaaccgc agtgcgctct ggttgtgcag cacctgttcc caccagtgcc caacgacgta
     2941 ctccggaatg aataccgtga cgacggtgcg cggcgactcc ttggtgaccc gcttgacata
     3001 gtcgagcacc ggtcgggtga tctcgcggta cggcgaggca atcaccttga gcggcacgct
     3061 gatatcgctg tcttcccatc ggtgcacgag ctcgcgcgtt tcggcgtcgt cgacgctgac
     3121 cgtgatggcc tccagcacgt cgggcctggt ggcccgggca taggccagcg cccgcttcgt
     3181 cggcaggtgc agcttggaca ccagcacgac ggcgtggttg cggctcggca acaccatgtc
     3241 atcgccctgt tcctcggctc gcctgtcgag ctcgtgggcg acggtgtcgt agtgcttgtg
     3301 gatcatcttc atgatcatga acagcgcgcc catcgccagg atcgcgatcc acgcgcccgc
     3361 gacgaacttc gtcaccacca cgatgatcag cacggtcccg gtgcagataa agccgatcgt
     3421 gttgatcacc cgcgaccgca ccatgtggcg acggacggcc gagtcggtct cggtgcgcag
     3481 cagccgcgtc cagtgccgaa ccatcccgat ctggctgagc gtaaaggaca cgaacacgcc
     3541 gacgatgtac agctggatca gcgcggtcac ctcggcgcgg aacgcgacca caaacgcgat
     3601 ggcggcgaag gccaagaaca ggatgccgtt ggagaacgcc agccggtcgc cgcgagtgtg
     3661 aagctgccgc ggcaggtaac ggtcttgggc cagaatcgat cccagcaccg gaaagccgtt
     3721 gaacgcggtg ttggcggcca gcaccaggat cagtgcggtg acgcccgcga tgaggtacag
     3781 ccctagcggg aagtcgtgaa aaaccgcgtc cgcgagctgc gcgatcagtg tcttctggtg
     3841 atagttcggc ggcgccccga ccagctgctc agggttttca gccatcttca cgccggtctc
     3901 cttggccagc atgatgatgc ccatgaacag tgacaccgcg atgacgccaa gcatcagcag
     3961 tgtcgtcgcg gcgttgcggg acttgggttt ccggaacgcc ggcaccccat tgctgatcgc
     4021 ttcgacaccc gtcagcgccg cgcagccgga cgagaacgcc cgtgccacca gaaagaccat
     4081 cgcgaaaccc agcacctcgc cgtgttcgga atgcacctcg aacccagctg attcggcgcg
     4141 cagtggattg cccagcacat agatctggaa caggccccag gccagcatga tgtacatgcc
     4201 gatcatgaac gcgtaggtcg gaaccgcgaa cgcggtgccc gattcgcgga tgccacgcag
     4261 gttcaacgac gccaaaatca ggatcgcgat gacggcgaac aacaccttgt gatggttgat
     4321 gagcggcacc gccgacccga tgttggccat cgccgatgcc atcgagaccg cgaccgtgag
     4381 cacgtagtcg actagcaggg cgctggccac cgtcaggccc gcggtgtggc ccagattggt
     4441 ggtgaccacc tcgtagtcgc cgccgcccga cgggtaggcg tgcacgttct gccggtagct
     4501 cgcgatcacg acgagcatga cggcaccgac ggcaagcccg atccacggtg ccatcgagaa
     4561 cgcggccagg cccgcgaccg acagcaccag aaagatttcc tccggcgcgt aggccaccga
     4621 cgacagcgcg tcggacgcga acaccggcag cgcgatccgc ttgggtaaca gcgtgtgggc
     4681 gagcttgtcg ctgcggaacg gcctccctag aaccaaccgg cgcgcagcgg tcgaaagctt
     4741 ggacacgaga gccaagagta agcccacggg cgaatggctg tagcgttccg agtgcgcagg
     4801 ttcgctacag acaggccgga aaggaccgtc aggtgcgtgt agtggtcatg ggatgcggcc
     4861 gggtgggcgc gtcactatca gacagcctgg cccggatcgg tcacgacgtg gccgtcatcg
     4921 atcgcgacag cacggcattt caccgcctgt cccccgagtt ttcgggtgag cgcgtgcttg
     4981 gcatgggctt cgaccgcgat gtgctgctgc gggcaggcat cgaggaggct ggtgcgttcg
     5041 cggccgtatc gtcgggcgac aactccaaca tcatctcggc gcgggtggcc cgcgagacgt
     5101 tcggcatcga gcgcgtggtg gcccgcatct acgacgccaa gcgcgcggcc gtctacgaac
     5161 ggctcggcat cccgacggtt gccaccgtgc cgtggacgac ggaccgtctg ctcaacgtgc
     5221 tgacccgcga gaccgagacc accaagtggc gcgacccgac cggcaacgtc ggcgtcaccg
     5281 agatggcgct tcacgaggag tgggccggcc accggatcac caacctggag gcggccaccg
     5341 gggcgcgcgt ggctttcctg atccggttcg gcgccggtct gctgcccgac ccgaagaccg
     5401 tcattcaagc cggcgatcag gtgtacatgg cagctatcgc cggacatatc gccgaagcat
     5461 tggccatagc ggcgctgccg ccaagcgagg acaccgaggc ccaatgaaag tcgccatcgc
     5521 cggggccggg gccgtcgggc ggtccatcgc gcgcgagctc gtcgagaccc atgacgtgac
     5581 gctgctggag cgcaaccctg accacatcga cgtcgacgcg atacctgcgg cgcactggcg
     5641 gcttggcgat gcgtgcgagc tgagcctttt ggagtcggtg aagctggagg agttcgacgt
     5701 cgtgatcgcc gcgaccggcg atgacaaggt caatgtggtg gtgagcctgc tcgccaagac
     5761 cgagttcgcg gtgccgcgtg tggtggcccg cgtcaacgac ccccgcaacg agtggttgtt
     5821 cgacgagaac tggggggtgg acgtcgcggt gtcgacgcca cgcatgctgg cgtcgctcgt
     5881 cgaagaagct gtcgccgtcg gcgatctggt gcggctgatg gagttccgca agggccaggc
     5941 caatctggtc gagatcacgc tgcccgacga cacgccgtgg ggcggaaagc cggtcaagcg
     6001 gctggatcta ccgcgcgacg cctcgctggt gacgattctg cgggggcccc gggtgatcgt
     6061 gccggaggcc gacgagccac tcgagggcgg cgacgagctg ttgttcgtgg ccaccaccga
     6121 ggtcgaggac gagctgcagg cactgctgct aaaccagcag tcgcgctgag ccctagcgcc
     6181 gggcgtaggc aggcggcggc gtcgcgcggt cgtggcagca cgccgcatcc tcgggtgata
     6241 cccggtcgaa atcgcattcg gcattgcgga tgccgatcgc tgagatgacg cccgcgaccg
     6301 cgaacagcgc cgccgtcacc aacgcaccct gccgaaaacc ggcgaaatcc attgcaccgc
     6361 cgatgattac accggtgaac gcgaccgcga tgaggcccgc gatccgggac actgcgttgt
     6421 tgatcgccga gccgatgccg ctctgg
//
LOCUS       DASRJA010000174         4558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_721629,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000174 DASRJA010000000
VERSION     DASRJA010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721629"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /gene="carB"
                     /locus_tag="VFC01_08090"
     CDS             <1..1523
                     /gene="carB"
                     /locus_tag="VFC01_08090"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZN79631.1"
                     /translation="CNPETVSTDYDTADRLYFEPLTFEDVLEVYYAESASGKGGPGVV
                     GVIVQLGGQTPLGLADRLEKAGVPIVGTSPKAIDLAEDRGAFGEVLRAAGLPAPKFGM
                     ATSFDQARRIAADIGYPVLVRPSYVLGGRGMEIVYDDETLEGYITRATQLSPEHPVLV
                     DRFLEDAIEIDVDALCDGAEVYIGGIMEHIEEAGIHSGDSACALPPVTLGRSDIESVR
                     RATEAIAHGIGVVGLLNVQYALKDDVLYVLEANPRASRTVPFVSKATAVPLAKACARI
                     MLGATISQLREEGVLAKSGDGAVAPRNAPVAVKEAVLPFNRFRKADGAQVDSLLGPEM
                     KSTGEVMGINHEFGSAFAKSQTAAYGSLPAEGTVFVSVANRDKRSLVFPVKRLADLGF
                     RVLATAGTAEMLRRNGIPCDEVRKHFEPPSEGRPALSAVDAIKAGEVDMVINTPYGNS
                     GPRIDGYEIRSAAVSMNIPCVTTVQGASAAVQGIEAGIRGDIDVMSLQELHSELGS"
     gene            1526..2344
                     /gene="pyrF"
                     /locus_tag="VFC01_08095"
     CDS             1526..2344
                     /gene="pyrF"
                     /locus_tag="VFC01_08095"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZN79632.1"
                     /translation="MTGFGRRLADAIAQRGPLCPGIDPHPELLRAWGLAADADGLSRF
                     CEICVTAFAEFAIVKPQVAFFEAYGAAGYAVLECTIGALQASDVLVLADAKRGDIGST
                     MAACADAWAGDSPLAADAVTASPYLGFGSLQPLLDTAKAYGRGVFVLAATSNPEGASV
                     QRAKTDGGTVAQSIVDAAAAVNRQSSPEPGSVGVVVGATLSDPPDLSALGGPVLVPGV
                     GAQGGRPDALGGFGGAHPGQLLPAVSRDVLRAGPDVAAVRAAAERFRDAVEYLA"
     gene            2341..2829
                     /locus_tag="VFC01_08100"
     CDS             2341..2829
                     /locus_tag="VFC01_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79633.1"
                     /translation="MTSYDIAEWADFANTVAGGAAALAGLLFVGLSLNLSEVLKYPGV
                     PARAAVTLGLTVAILLTAIFVATPGQDHRVLAAEVGVVGLATVVGVIVGLRQQRKGQH
                     PARALTSTLLLGVPALLLIVAAVSLWLQRGGGLYWVTAAVATGFVSATANAWVLLVEI
                     KR"
     gene            complement(2819..3193)
                     /locus_tag="VFC01_08105"
     CDS             complement(2819..3193)
                     /locus_tag="VFC01_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79634.1"
                     /translation="MRQAFQAFLALFGVIVIAISLAHLAIGPRAIIGAVAVNPTVFVH
                     WDRAAVVLTRCASETHLRESAGGGLLRRRDRPVPSFAWAHLGVVLDGTPNGFYLAMLA
                     VELALPPLMVVVAGRVAEPVSV"
     gene            3721..4035
                     /gene="mihF"
                     /locus_tag="VFC01_08110"
     CDS             3721..4035
                     /gene="mihF"
                     /locus_tag="VFC01_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HZN79635.1"
                     /translation="MALPQLTDEQRAAALEKAAAARRARAELKDRLKRGGTNLKQVLK
                     DAETNEVLGKMKVSALLEALPKVGKVKAQEIMTELEIAPTRRLRGLGDRQRKALLEKF
                     DQ"
     gene            4043..>4558
                     /gene="gmk"
                     /locus_tag="VFC01_08115"
     CDS             4043..>4558
                     /gene="gmk"
                     /locus_tag="VFC01_08115"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZN79636.1"
                     /translation="MSEGGGPGRAERLKAPVLVLSGPSAVGKSTVVRCLRERVPDLHF
                     SVSVTTRAPRPGEVDGVDYSFVTAERFQQLIDDGALLEWAEIHGGLHRSGTPAGPVRA
                     ATAAGKPVLIEVDLAGARAVKQAMPEVITVFLAPPSWEALESRLSARGTETPDVMARR
                     LDTARAELAAQD"
BASE COUNT          766 a         1509 c         1536 g          747 t
ORIGIN      
        1 actgcaaccc cgagacggtg tctaccgact acgacaccgc cgaccggctg tatttcgagc
       61 cgctgacgtt cgaggacgtg ctggaggtgt actacgccga atccgcctcg ggcaagggcg
      121 gtcccggcgt cgtcggcgtc atcgtgcagc tcggcgggca gacgccgctc ggtttggcgg
      181 atcgcctcga gaaggcaggc gtgccgatag tcggcaccag ccccaaggcg atcgaccttg
      241 ccgaggaccg cggtgcgttc ggcgaagtac tgcgggccgc gggcctgccg gcacctaagt
      301 tcggcatggc caccagcttc gatcaggccc gccgcatcgc cgccgacatc ggctacccgg
      361 tactcgtccg gccgtcctac gtgctgggcg gtcgcggcat ggagatcgtc tacgacgacg
      421 agacgctcga gggctacatc acccgtgcca cccagctgtc gcccgaacat ccggtgctgg
      481 tcgaccggtt cctcgaggac gccatcgaga tcgacgtcga cgcgctctgc gacggtgccg
      541 aggtgtacat cggcgggatc atggagcaca tcgaggaggc tggcatccac tccggggact
      601 cggcgtgcgc gctgccccca gtgacgttgg gccgcagcga tatcgagtcg gtgcggcgcg
      661 ccaccgaggc gatcgcccac ggtatcgggg tggtcggcct gctcaacgtt cagtacgcgc
      721 tcaaggacga cgtgctctac gtgctggagg ccaacccacg tgcgagccga accgtgccct
      781 tcgtctccaa ggccaccgcg gtgccactcg cgaaggcgtg tgcgcgaatc atgctgggcg
      841 ccaccatctc ccaattgcgc gaggagggtg tcctcgccaa gagcggagac ggtgccgtcg
      901 caccgcgcaa tgcgcccgtc gccgtcaagg aagccgtgct gccgttcaac cggttccgca
      961 aggccgacgg tgcgcaggtc gactcgctgc tcggcccgga gatgaagtcg accggcgagg
     1021 tgatgggtat caatcacgaa ttcggcagtg cattcgccaa gagccagacc gccgcctatg
     1081 gctcattgcc cgccgagggc acggtgttcg tgtcggtggc caaccgcgac aagcgctcgc
     1141 tggtgttccc ggtaaagcgg ctggccgatt tgggtttccg ggtgctggcc accgcaggca
     1201 ccgcggagat gttgcgccgc aacggcattc cctgtgacga ggtgcgtaag catttcgaac
     1261 cgcccagcga gggcaggccc gcgctctcgg ccgtcgacgc catcaaggcg ggcgaggtcg
     1321 atatggtgat caacaccccg tacggcaact ccggccctcg catcgacggc tacgaaatcc
     1381 gttcggcagc agtgtcgatg aacatcccct gcgtgacgac cgtgcagggc gcgtccgccg
     1441 cggtgcaggg cattgaggcc ggcatccgcg gcgatatcga cgtgatgtcg ctgcaagagt
     1501 tgcacagcga gctgggttca taaccgtgac cggattcggt cgccggctgg ccgacgcgat
     1561 cgcccagcgt ggcccgctat gtcccggcat cgatccgcat cccgagctgt tgcgggcgtg
     1621 ggggctggca gccgacgccg acgggctcag caggttctgc gaaatctgcg ttacggcgtt
     1681 cgccgaattc gccatcgtca agccgcaagt ggcgttcttc gaggcctacg gcgcggccgg
     1741 ttatgcggtg ctggaatgca ccatcggtgc gctgcaggcg tctgatgtgc tggtgctggc
     1801 cgacgcgaaa cgcggcgaca tcggctcgac gatggcggcc tgcgccgacg cgtgggcggg
     1861 tgattcaccg ctggccgccg acgccgtgac ggcctcgccg tatctcggat tcggttcgct
     1921 tcaacccctt ctcgacaccg ccaaggcata tggccgcggc gtgttcgtgt tggccgcgac
     1981 gtcgaacccc gagggcgcca gcgtgcagcg agccaagacg gacggcggca ccgtcgcaca
     2041 atcgatcgtc gacgcggcgg cggccgtcaa ccgccagtca tcgcccgagc ccggctcggt
     2101 cggcgtcgtc gtcggcgcca cgctgtccga cccgcccgac ctcagcgcgc tgggcggtcc
     2161 cgtcctggta cccggcgtcg gcgcgcaggg cggacggccc gacgcgctcg gcggtttcgg
     2221 cggggcccat cctgggcagc tcttgcccgc ggtgtcccgc gatgtgctgc gtgccggccc
     2281 cgacgtggct gccgtacgcg ccgccgcgga gcgcttccgt gatgcggtgg agtatctggc
     2341 atgacctcct acgacatcgc ggaatgggcc gacttcgcga acaccgtcgc cgggggagcg
     2401 gccgcgctgg cgggcctgtt gttcgtcggg ctctcgctga atctctccga ggtgctgaag
     2461 tatccgggcg tcccagcccg cgccgcggtc acgctcgggc tcaccgtggc gatattgctg
     2521 accgcaatct tcgtcgccac gcccggccaa gaccatcgcg tgctggccgc cgaggtcggc
     2581 gtcgtcggcc tggcgacggt ggtgggcgtg attgtcggtc tgcggcaaca gcggaagggc
     2641 cagcacccgg cgcgcgctct gacgtcgacg ctgttacttg gagtcccggc gctgctgttg
     2701 atcgtcgccg ctgtttcgct gtggctgcag cgcggcggcg gtctctattg ggtaactgca
     2761 gctgttgcaa cgggtttcgt gtctgcgacc gccaacgcct gggttctgtt ggtcgagatc
     2821 aaacgctgac aggctcagcg actcgcccgg ccacgacgac catcagcggc ggcagcgcca
     2881 gctcgacggc gagcatcgcg agatagaacc catttggcgt gccgtcgagc acgaccccaa
     2941 ggtgggccca cgcgaagctg gggaccggcc gatcccgccg acgaagaaga ccgccgccag
     3001 cagattcacg tagatgcgtt tccgatgcac atcgcgtgag caccacagca gcgcgatccc
     3061 agtgcacaaa aacagtcggg ttcaccgcga cggcgccgat gatcgctcgc gggccgatcg
     3121 ccaaatgcgc gagcgaaatg gcgatcacga tgacaccgaa cagggcgaga aacgcttgaa
     3181 aagcctgtcg catggaccgc tcccagtgcc gcgaatggat ctactacgca ctgtagttga
     3241 ccgcgcctag gctgccaagg atcaccaaac ggcaacactg gcaccatcag acgcatgagt
     3301 cacaccaatg tcgtttgaat gcaaacggcg tggcctttcc tgggcggaat acctgtcaac
     3361 tgaccggccg cgacacgccc gacacaccat gaccaggcaa aattttattt ttggaccctc
     3421 gtgcgaactt gggtggcggg cgacgttaca ccccgaccgc ggcggtgaca gctgccacta
     3481 accgcgaaaa cccttgatat gcaggcactt ccaccgaaat cgggcgaaaa atcgcgtcac
     3541 cgccgccatc cgacgcagct ttgcgcggcc tgcgcgtgcg cactacacgc tgggcttttg
     3601 acccgataac cagggggtgg ggttagcttt cgtcagaatg cgtgggtacg gtcgtcgtcg
     3661 ctggctgatt tacgggtcag ccgagacaaa acagatgatg gtgacgagac ggaggaaccc
     3721 gtggcccttc cccagttgac cgacgaacag cgcgcggcag cgttggagaa ggctgctgcc
     3781 gcacgtcgag cacgtgccga actcaaggat cggctcaagc gcggcggcac caacctcaag
     3841 caggtgctca aggatgccga gaccaacgag gtcttgggca agatgaaggt ttctgccctt
     3901 ctggaggcgt tgcccaaggt cggcaaggtc aaggcgcagg aaatcatgac cgaactcgag
     3961 atcgccccca cccgccgcct gcgcggcctg ggcgaccgtc agcggaaggc gctgctggag
     4021 aagttcgacc agtagcgagt cagtgagcga aggcggaggg ccgggcaggg ctgagcgcct
     4081 caaggcaccg gtcttggtgc tgtccggccc gtctgccgtc gggaaatcga ccgttgtccg
     4141 ctgcttgcgt gaacgcgttc ccgacctgca cttttccgtt tccgtcacca ccagggcgcc
     4201 ccgccccggt gaggtggatg gcgtcgacta ctcgttcgtg accgccgagc ggttccagca
     4261 gctgatcgac gacggcgccc tgctggaatg ggcggaaatc cacggtggcc tgcatagatc
     4321 cggaacgccg gccgggccgg tccgcgcggc gaccgccgcc ggcaagcccg tcttgatcga
     4381 ggtcgaccta gccggcgcgc gcgccgtcaa acaagcaatg cccgaggtca tcaccgtctt
     4441 cctggcccca ccgagttggg aggcgctgga gagccggctg tctgcgcgtg gcaccgagac
     4501 cccggatgtg atggcccgca ggctcgacac cgcgcgggcc gaattggccg ctcaggac
//
LOCUS       DASRJA010000175         2856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4105037,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000175 DASRJA010000000
VERSION     DASRJA010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4105037"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VFC01_08120"
     CDS             complement(<1..357)
                     /locus_tag="VFC01_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HZN79637.1"
                     /translation="MRRFLIVGCGGSGGATLAFIMDQLRSELHSAGIEKLLGGWQFVQ
                     IDVPSAPETGPEGLANVPEQGGTYIGCGPQGSSYAILDGALSQRLTAESALDTTATWA
                     PRNPQEVSIPISAGAGQ"
     gene            complement(358..>2856)
                     /locus_tag="VFC01_08125"
     CDS             complement(358..>2856)
                     /locus_tag="VFC01_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN79638.1"
                     /translation="VLLFDESSSLQGTDPQAARVQAARYLLQTLGRYADRVGANLEVA
                     TSGFADTYTREQDWTKLTGSTADQVGAQLSSIATKNTGIDTDYWLALDGARQELASRG
                     QGPNGGQRCQAIAWFSDGKIDFTSRATSKPYADGVTLDSPTGVEDTTKRAVESICRPG
                     GVADQLRSSGIIMLGIGLGANKTPSDFDVMSAISTARGLNGMACGEITDPIPGDFYPV
                     SNIDDMLFGFDALNPDPGTTGQGPVCQLRVCPEARHNFTLDRSIKSVDILGSGGVAGV
                     VPYLISPSGKTLELPKKDGRNTADIDGVPVTYEWRSDSAQTISLQNTGGPQWPGQWAI
                     VYVDTTGQHPDAVSRVSIHITTDIFPALTNADKVPWRSGQVVKGLTFGLVDGQGKPML
                     PTDLAGTATMSVALAPDGAAPIPLLDSVAKADIANPVDADLTKVNPGRALLRMSLVIT
                     TAPATDPSGAQIAPGTQLSPQDVEVPVQILPKLGLPVPGERIDFGTVQAAKGATASLN
                     ITGPGCAWMADTDAAKVVAAPDDIGTARLSSTANSAQNCVKVQAGQTGQLSVTLRTDR
                     DGHGGLNGTVPIHVAALDNPGDAQVIDVPFVASLIKPLNTTNFVLVFLAALLLGPGIP
                     LALLYAAKWWVSKIPDTPMLADRIPVEVNNDVVLRDGEPFTPADTDLIRPVPGLAGGA
                     RKVSVQGVTLTTTVGRSPFGPGYVTVDADGLVSVGSALPATDRSGLRAVLPLAVHNKW
                     VVLHDPRGPANVADVLLLVGGRTDTAARERLYEDVGRRLPELLNGLRLRAVEAGLAVS
                     DDEARQAVSPFGEPAQGGSAAHDPDPFDPYREGN"
BASE COUNT          429 a          963 c          978 g          486 t
ORIGIN      
        1 ctggccggcg ccggccgaga tcgggatgga cacctcctgc gggttgcgcg gcgcccaggt
       61 ggcggtggtg tccaacgccg attcggcggt cagccgctgc gacagtgccc cgtcgaggat
      121 cgcgtagcta ctgccctgcg gtccgcagcc gatgtaggtg ccgccctgtt cgggaacgtt
      181 ggccagtccc tcagggcccg tctccggcgc gcttggcacg tcgatctgaa cgaattgcca
      241 accgcccaag agtttttcga tgcccgcgga gtgcagttcg gagcgcagct gatccatgat
      301 gaacgccagc gtggcaccgc cggaaccacc gcatcccact atgaggaatc gtcgcattca
      361 gtttccttcc ctgtacgggt cgaacggatc tgggtcatgg gccgccgacc cgccctgcgc
      421 gggttcaccg aacggcgaaa ctgcttgtcg cgcttcgtcg tccgagacag ccagcccggc
      481 ttcaaccgcc cgcaaccgca gcccgttgag cagctcgggc agtcgacggc cgacgtcttc
      541 gtagagccgc tcacgtgccg cggtgtcggt gcgtccgccg accagcagca gcacatcggc
      601 gacgttggcc ggtccgcggg gatcgtgcag gaccacccac ttgttgtgca cggccagcgg
      661 cagcaccgcg cgcagcccgc tgcggtcggt tgcgggcagc gccgaaccca cactgaccag
      721 accgtcggcg tcgacggtca cgtagccggg gccgaagggg ctgcggccga cggtcgtggt
      781 cagcgtgaca ccttgcaccg agactttgcg agcacccccg gccaggccgg gcaccgggcg
      841 aatcaggtcg gtgtcggccg gcgtgaaggg ttccccgtcg cgcagtacca catcgttgtt
      901 cacctcgacc gggatccgat cggccagcat cggcgtgtcg gggatcttgc tgacccacca
      961 cttcgccgcg tacagcagtg ccagcgggat ccccggaccc agcagcagcg cggccaggaa
     1021 caccagcacg aagttggtgg tgttcaacgg cttgatcagc gacgcgacga acggaacatc
     1081 gatgacctgt gcatcgcccg ggttgtcaag cgccgcaacg tgtatcggca cggtcccgtt
     1141 cagcccgccg tgtccgtcgc ggtcggtgcg cagcgtcacc gagagctgcc cggtctgccc
     1201 ggcctgcacc ttcacgcaat tctgcgcgga gttcgcggtg gaggacagcc gcgcggtgcc
     1261 gatgtcatcg ggcgcggcca ccaccttggc ggcatcggtg tcggccatcc aggcgcagcc
     1321 tgggccggtg atgttgagcg acgcggtcgc gcccttggcc gcctgcacgg tgccgaaatc
     1381 gatccgctcg cccggcaccg gcaggccgag cttgggcagg atctgcacgg ggacttcgac
     1441 gtcctggggt gacagctgag tgccgggcgc gatctgagcg ccggatgggt cggtggccgg
     1501 cgcggtggtg atcaccaacg acatccgcag cagcgcgcgc ccgggattca ccttcgtcag
     1561 gtcggcgtcc accgggttgg cgatgtcggc tttggccacc gaatccagca gcgggatggg
     1621 cgccgcgccg tcgggcgcca gcgccaccga catggtcgcg gtcccggcaa ggtcggtcgg
     1681 aagcatgggc ttgccctgcc cgtcgaccag accgaacgtc agacccttga ccacctgccc
     1741 gctgcgccaa ggaactttgt cggcgttggt aagcgccgga aagatgtcgg tggtgatgtg
     1801 aatgctgacc cgcgataccg cgtcggggtg ctgaccggtg gtgtcgacgt agacgatcgc
     1861 ccactgcccc ggccactgcg ggccgccggt gttctgcagc gaaatggtct gcgctgaatc
     1921 ggatcgccac tcatacgtca ccggcacgcc gtcgatgtcg gcggtgttgc ggccgtcctt
     1981 cttgggcagt tccagcgtct tgcccgacgg cgagatcagg tagggcacca caccggccac
     2041 tccgccggaa ccgaggatgt ccaccgactt gatcgagcgg tccaacgtga agttgtgccg
     2101 cgcctccggg cacacgcgca gctggcagac cgggccttgc ccggtggtgc cggggtccgg
     2161 gttgagcgcg tcgaatccga acagcatgtc gtcgatgttg gagacgggat agaagtcacc
     2221 cggtatcgga tcagtgatct ccccgcacgc catgccgttg agtccgcggg ccgtgcttat
     2281 cgccgacatc acgtcgaagt cgctgggtgt cttgttggcg cccaggccga ttcccagcat
     2341 gatgatgccg ctgctgcgca actggtcggc gacgccgccc ggccggcaga tcgactccac
     2401 ggcccgtttg gtggtgtcct cgacaccggt cgggctgtcg agggtgaccc cgtcggcgta
     2461 gggcttggac gtcgcccgcg acgtgaaatc gatcttgccg tcggagaacc atgcgatcgc
     2521 ttggcagcgc tgtccgccgt tgggcccttg cccgcgcgac gccaactcct ggcgggcccc
     2581 gtcgagcgcc agccagtagt cggtgtcaat accggtgttc ttggtggcga tgctcgacag
     2641 ctgcgcacct acctggtcgg cggtggatcc ggtcagcttc gtccaatcct gttcgcgtgt
     2701 ataggtgtcg gcgaacccgg aggtggcgac ctcgagattc gcgccgaccc ggtcggcgta
     2761 tctgcccagc gtctgcagca ggtagcgcgc cgcctgcacc cgggccgcct gaggatcggt
     2821 gccctgcagg ctgctggact cgtcgaacaa cagcac
//
LOCUS       DASRJA010000176         2405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4104829,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000176 DASRJA010000000
VERSION     DASRJA010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4104829"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VFC01_08130"
     CDS             complement(<1..646)
                     /locus_tag="VFC01_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZN79639.1"
                     /translation="MKTKAAVLWGLNQKWEVEEVDLDGPKHGEVTVKLAASGLCHSDY
                     HIVTGDMPRPLPCVGGHEGAGIVVEVGKGVHDLAEGDPVVFSFLPACGRCRPCASGMS
                     NMCELGAYLMLGPQIDGTYRFHARGQDVGQNCVLGAFSEYTTVPEASVIKIDASIPLD
                     KAALVGCGVTTGYGSAVHTGEARDGDTVVVMGVGGIGINAVQGARIAGARNVIAL"
     gene            complement(699..2105)
                     /locus_tag="VFC01_08135"
     CDS             complement(699..2105)
                     /locus_tag="VFC01_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN79640.1"
                     /translation="MTTDVVFPQRERYTDDLGGMLAALRKVHASGRTRPLAWRLEQLA
                     GIERLVKEREADFAEALREDLGRSAVEAWLGDLAGSAGEATYARKNLKKWMKRKKQRM
                     PLSQLPGAGWVQCDPLGVVLVIGPWNYPVFLSLSPLVAAVAAGNCAVLKPSELAPATS
                     ALLARLIPQYVDPDAVKVIEGDGATTQELLSLGFDHALFTGGIEIGKKIMAGAAETLT
                     PVTLELGGKSPVIVSSDADIDVVARRIAWVKLMNSGQTCIAPDYVLAHNTIRDELVTK
                     ITAALAQFQAGADPRGLRIVNERQFDRLAGYLAATKGTVVAGGGADRGQLLIQATVLV
                     DPDPNEPAMTDEIFGPILPVLSVDSDQVAIDFVNSRPKPLALYVFTKSKTTGERLIDA
                     IPSGGAVLNHVAMHCLVPALPFGGVGSSGMGAYHGRWGFEALSHRRAVLAKPSRPDPG
                     FVYPPYTDRAIAIMRRLF"
     gene            complement(2137..>2405)
                     /locus_tag="VFC01_08140"
     CDS             complement(2137..>2405)
                     /locus_tag="VFC01_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN79641.1"
                     /translation="LQIHGSIGVTDEMPFLGFIRDSFNMALADGPTEVHLTNLARAIL
                     RGYEPDEDVFPDYHTPRRLERARIKYADVLRRHGLQPAAHTASV"
BASE COUNT          406 a          782 c          774 g          443 t
ORIGIN      
        1 cgagcgcaat gacgttgcgc gcacccgcga tccgggcacc ctgcacggcg ttgatcccga
       61 tgccaccgac tcccatgacg accaccgtgt cgccgtcacg cgcctctccg gtgtgtaccg
      121 cgctgccgta gccggtggtc accccgcaac cgaccagcgc cgccttgtcc agtgggatgg
      181 aagcatcgat cttgatgacc gacgcctccg gaacggttgt gtactcggag aacgctccaa
      241 gtacgcagtt ctgaccgacg tcctggccac gggcgtggaa gcggtaggtt ccatcgatct
      301 gcggtccgag catcaggtag gcccccagtt cgcacatgtt gctcatcccg ctcgcgcacg
      361 gtcggcatcg gccacacgcg ggcaggaaac taaagacaac cgggtcaccc tcggcgaggt
      421 cgtgcacacc tttgccgacc tccaccacga tgccggcgcc ctcgtgcccg ccgacgcacg
      481 gtagaggtcg gggcatgtct ccggtgacga tgtggtagtc ggaatggcac aggccactag
      541 cggccagctt gacggtgact tcgccgtgct taggcccgtc gaggtcgact tcctcgacct
      601 cccacttctg gttcaaaccc catagcacgg ccgctttggt cttcactgtc gtcgcttcct
      661 aactcttgtc tcatcacgcg ggcgcctggt tggtggcgtc aaaacaatct gcgcatgatc
      721 gcgatggcgc ggtcggtgta gggcggatag acgaatcctg ggtctggccg actcggtttg
      781 gcgagcaccg cacggcgatg gctcaaagcc tcgaaacccc accggccgtg atacgccccc
      841 ataccgctgg accccacgcc accgaacggc agtgcaggta ccagacagtg catggcgacg
      901 tggttgagga ccgcaccgcc ggaggggatg gcgtcgatca ggcgctcgcc cgtcgtcttg
      961 gacttggtaa agacatagag cgccaacggc ttgggtcggg agttgacgaa gtcgatcgcc
     1021 acctgatccg aatccacgga cagcacgggc aggatcggcc cgaagatctc gtcggtcatc
     1081 gcaggttcgt tgggatcggg gtccaccagt accgtcgcct ggatgagcag ttgaccgcgg
     1141 tccgcgccgc cgccggccac cacggtgccc ttcgtcgcag ccaggtaacc cgccaaacgg
     1201 tcgaactgtc gctcgttgac gatccgtaga ccacgggggt cggcgccggc ctggaactgc
     1261 gccagcgccg cggtgatctt ggttaccagc tcatcgcgaa tcgtattgtg ggccaataca
     1321 taatcgggcg cgatgcaggt ctgaccggag ttcatcagtt tgacccacgc gatgcggcgc
     1381 gcaactacgt cgatgtcggc gtcactggaa acgatgaccg ggctcttgcc gccgagttcg
     1441 agggtcactg gggtcaaggt ttcggccgcc ccggccatga tcttcttgcc gatctcgatg
     1501 cccccggtga acagggcatg gtcgaagccc agcgacagca actcttgggt tgtcgcgccg
     1561 tcgccctcga tcaccttgac ggcgtcggga tcgacgtact gcggaatgag ccgtgcgagc
     1621 agcgccgagg tcgccggtgc gagctcggac ggtttgagga ccgcgcaatt tcccgccgct
     1681 acggcggcga ccaatgggct caggctcagg aacaccgggt agttccacgg gccgatgacc
     1741 aggaccactc ctagcggatc gcattgcacc cagccagcac cgggcagttg gctcagcggc
     1801 atgcgctgct tcttgcgctt catccacttc ttgaggttct ttcgcgcata ggttgcctca
     1861 cccgcggagc cggccaagtc ccccagccag gcctcgaccg cactgcgccc gaggtcttct
     1921 cgcagagcct cggcgaagtc ggcttcccgc tccttgacta ggcgttcgat gccggcgagc
     1981 tgctctaggc gccaggcaag cggacgcgtg cggccgctcg catgcacctt ccgcagtgcg
     2041 gcgagcatgc ccccaagatc atcggtgtac cgttcccgct gggggaaaac cacgtcggtc
     2101 gtcatcttcg cttcctgttc atgtacttcg cggtggtcaa acgctggcgg tgtgcgctgc
     2161 tggctggagg ccgtggcgac gcagaacgtc ggcgtacttg attcgcgccc gctcgaggcg
     2221 gcgcggtgtg tggtagtcgg ggaagacgtc ctcgtcgggt tcatatccgc ggaggatggc
     2281 gcgggcaagg ttggtcaggt ggacctcggt cgggccgtcg gcgagcgcca tgttgaagga
     2341 gtcacggatg aatccgagga acggcatttc atcggtgacg ccaatcgatc cgtggatctg
     2401 cagcg
//
LOCUS       DASRJA010000177        10576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4104821,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000177 DASRJA010000000
VERSION     DASRJA010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4104821"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFC01_08145"
     CDS             complement(<1..404)
                     /locus_tag="VFC01_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZN79642.1"
                     /translation="MKITGTAVKLGIFSLVLLLFTAMLIVVFGQMRFDRTTGYSAIFS
                     NTSGLRAGQFVRASGVEVGKVSKVELLDGGSQARVDFDVDRSLPLFEGTTASVRYLNL
                     IGDRYLELKRGDSDKRMPAGGTIPIEHTEPAL"
     gene            complement(401..1678)
                     /locus_tag="VFC01_08150"
     CDS             complement(401..1678)
                     /locus_tag="VFC01_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN79643.1"
                     /translation="MTAPLNKPRTPPFKLAGLILTLLTVVVLVLVYVLFRGYNLDREQ
                     LTMISSRAGLSMDPGAKVTYNGVEIGRVAKVEEITVGDQPRAKVILDVNPKYFSLIPK
                     NVNADITATTVFGNKYISFSSPKDPSPQRITTSDVIDVTSVTTEFNTLFETVVEVAQQ
                     VDPIKLNQTLSATAESLNGLGDRFGQSIIHGNEILADINPQMPQIRRDNQLLADLGDV
                     YANAAPDLFDGLENAVTTARTLNEQQANIDQALMASIGFGNTGGDIFERSGPYLVRGT
                     EDLVPTSQLLDTYSPALFCTIRNFHDVEPKVAASLGGNGYSLRTTSELIGAGNAYVYP
                     DNLPRVNARGGPEGRPGCWQPITRDLWPAPYLVMDTGASIAPYNHIELASPFGCAGLP
                     PCLIPLLPAPIPLIIPGIEYVWGRQFGENTINP"
     gene            complement(1682..2551)
                     /locus_tag="VFC01_08155"
     CDS             complement(1682..2551)
                     /locus_tag="VFC01_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN79644.1"
                     /translation="MSTTQVLRSRFPRGVARAEKVAGAPARGLDSVGHVAWFVVTSMG
                     SIGHALRYYRKETLRLIAEIGMGTGAMAVVGGTAAIVGFVSLSGSSLVAIQGFASLGN
                     IGVEAFTGFFAAMINVRSAAPLVSGVALAAVVGAGATAELGAMRISEEIDALEVMGIK
                     SISYLVSTRIMAGFVVIIPLYAMAMILSFLSGQVTTTLFYGQSTGTYEHYFSTFLRPE
                     DVFWSFVDAIIISIIVMLNHCYYGYYASGGPVGVGEAVGRAMRASLVAVALVVLFVAL
                     ALYGVDPNFNLTV"
     gene            complement(2553..3350)
                     /locus_tag="VFC01_08160"
     CDS             complement(2553..3350)
                     /locus_tag="VFC01_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN79645.1"
                     /translation="MTASTGVVDYVRDQTRPALTAVGGFVRMCVLTGKALFRPPFQWR
                     EFILQSWFLFRVSFLPTLAVSAPLTVLLIFLLNILLAEFGAADVSGAGAAIGAVTQLG
                     PLVTVLVVAGAGSTAICADLGARTIREEIDALEVLGIDPIHRLVVPRVVASTFVALLL
                     NGAVITIGLVGGFVFGVYLQNVSAGAYVSTLTLITGLPEVLISVVKATVFGLIAGLVG
                     CYRGLTVSGGAKGVGTAVNETLVLCVIALFAVNVVLTTIGVRFGTGR"
     gene            complement(3563..5194)
                     /gene="fadD5"
                     /locus_tag="VFC01_08165"
     CDS             complement(3563..5194)
                     /gene="fadD5"
                     /locus_tag="VFC01_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase FadD5"
                     /protein_id="HZN79646.1"
                     /translation="MTEPPPTAQPYLARRQNWANQLARHALMQPNAVALRFLGHTTTW
                     ADLDRRVGSLASALSRRGVSLGDRVLILMLNRTEFIESFLAATRLGAIAVPVNFRMTP
                     PEIAFLVSDCQARVVVTEAVLAKVATAVRDIDPTLATVIVAGAGTDDEVVGYDDLIAE
                     EGAAGPDPIDIPNDSPALIMYTSGTTGRPKGAVLTHTNLAGQAMTNLFTIGADINRDV
                     GFVGVPLFHIAGIGNMITPLQLGLPTVLYPLGAFDPGALLDVLEAEQVTSMFLVPAQW
                     QAVVAEQRINPRKLVLRVLSWGAAPASDTLLRDMAETFPGTQIYAAFGQTEMSPVTCM
                     LLAQDAIRKLGSVGKVIPTVAARVVDENMNDVPVGQVGEIVYRAPTLMAGYWNNPKAT
                     AEAFAGGWFHSGDLVRQDDEGYVWVVDRKKDMIISGGENIYCAEVENALAGHPAIVEV
                     AVIGRPDPKWGEVPVAVAAVHTDLDLADLDEFLTERLARYKHPKALEIVDALPRNPAG
                     KVLKTELRARFGAQELIDAGESSTPPTVSAGAQDN"
     gene            5321..6013
                     /locus_tag="VFC01_08170"
     CDS             5321..6013
                     /locus_tag="VFC01_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN79647.1"
                     /translation="MNAPARPRAQRRRAVRREQLSDEVAARLRTDIMTGTLRPGTFIR
                     LDETAAALGVSITPVREALRTLRGEGMVQLEPHRGHVVVPLTRGDIEDIFWLQATIAK
                     ELAATAAERITDAQIDELERLNEGLAAAVESGDPEEVVAAEFAFHRAFNRATGRIKLA
                     WFLLHVARYLPGQIYASDAAWGAAAVAGHRELIAALRRRDVDTVVRLTSGEFSDAAQR
                     LVARLDEIGLWS"
     gene            complement(6020..6463)
                     /locus_tag="VFC01_08175"
     CDS             complement(6020..6463)
                     /locus_tag="VFC01_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN79648.1"
                     /translation="MPLVSKTVEVAAPAESIMAIVADFESYPLWNEEIKGCWVLARYE
                     DGRPSQLRLDVVVQGQSGTFITAVYYPAENQIYTVLQQGEFFEKQEQKFSVVPIGATS
                     LLTVDFDVETKLPIPKPMVKKAIGDTLDYLADNLKARAEQLAASP"
     gene            complement(6438..6896)
                     /locus_tag="VFC01_08180"
     CDS             complement(6438..6896)
                     /locus_tag="VFC01_08180"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZN79649.1"
                     /translation="MTSEESLTSRDFPVHWPVLTRWTDNDMFGHLNNAVYYELFDTAI
                     NAWINTSCAIDPLSVPWQGVVAESGCKYSSELKFPDPLTVGLAVARLGNSSVTYRLAL
                     FQKDGPVAAVGHWVHVYIDRTTRRPVPIPASIRSLLESICSADAARKQDS"
     gene            6932..8038
                     /locus_tag="VFC01_08185"
     CDS             6932..8038
                     /locus_tag="VFC01_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZN79650.1"
                     /translation="MRIRGAVLEEIGRPRPYAESRPLSISELDLAPPGDSELLVRIET
                     AGLCHSDLSVVDGNRVRPVPMLLGHEAAGIVEQAGSAVADVPVGQRIVMTFLPRCGQC
                     AACATNGLTPCEPGSAANGAGTLMTGERRLSRDGQPIHHHLGVSGFATYAVVDRRSVV
                     PVPADVPPSVAALLGCAVLTGGGAVINAGRPQAGQTVVVVGLGGVGMAAVITALAHDD
                     VTVIGVDQLADKLDLARSMGVHEAYTPDEARERGVKAAVVIEAVGHPLAVQTAIDLTA
                     PGGRTVTVGLPSPDARITVSPLGFVAEGRSLIGSYLGSAVPSHDIPHFVGLWRSGRLP
                     VEKLVSSTINLDEINTGMDQLADGHAVRQLISFD"
     gene            complement(8025..8879)
                     /locus_tag="VFC01_08190"
     CDS             complement(8025..8879)
                     /locus_tag="VFC01_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZN79651.1"
                     /translation="MPTTLRDLSQWLLTNGLHIVLVVLFAVITTRLIRWIAGRISKRL
                     DADDGADAVLRPERVKHRQAVASVISSVAIALLYVVVSIDVANQLGLPVGSLVAPAAV
                     LGAALGFGAQRIVQDLLSGFFLITEKQYGFGDLVELTVTAGGAAVGTVEEVTLRVTKL
                     RTSDGEVFTVPNGQIVKSLNMSKDWARAVVDIPVPAAADINKVTDVLRNVSTTAMQDD
                     GLPDLLLDEPQLMGVESIEVDTLNMRMVARTLPGKQFEVGRRLRALVVAALRRAGIAT
                     TEAVTSQS"
     gene            8979..10100
                     /locus_tag="VFC01_08195"
     CDS             8979..10100
                     /locus_tag="VFC01_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZN79652.1"
                     /translation="MSDRIRHLKLSHVVLPLETPVSDAKVLTGRQEPLTETVLLFVEV
                     LTEEGHEGTGFSYSKRAGGPAQYTHLAEIADVALGQDPSDIDRIYQSLLWAGASVGRS
                     GVATQAIAALDVALWDLKARRAGLPLAKLIGAHRDSCPVYNTSGGFLQASVAEIKEKA
                     TASLASGIGGIKIKVGQPDWKIDLHRVAVLREHLGETPFMVDANQQWDRARARRMCRE
                     LEAFGLVWVEEPLDAWDAVGHADLSRTFDTPIATGEMLTSVSEHMALLDAGYRGIVQP
                     DAPRIGGITPFLKFATLAAHAGLALAPHYAMEIHLHLAATYPTEPWVEHFEWLNPLFN
                     ERIEIRDGRIWVPDRPGLGFTLSEQMRALTKETADFGAA"
     gene            10097..>10576
                     /locus_tag="VFC01_08200"
     CDS             10097..>10576
                     /locus_tag="VFC01_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN79653.1"
                     /translation="MTTTLGQRVVAGLKDKILAGDLPPGHKLPSEAELIDEYGVSRTV
                     VREAVTRLRAEGLVETFQGRGSFVLAMPEPTSFTVESAAIRTHHDVLDMIDFRLGIEC
                     EAAALAAARADTSGAESIRAALDAFVAAAPEDQVEADFGFHRAIAAASGSRFYLELLD
                     "
BASE COUNT         1881 a         3481 c         3491 g         1723 t
ORIGIN      
        1 tcgagcgcgg gctcggtgtg ctcgatcggg atggtgccgc cggccggcat ccgcttgtcg
       61 ctgtcgccgc gcttgagctc caagtaccgg tcgccgatca ggttcaggta gcggacagac
      121 gcggtcgtcc cctcgaacag cggcagcgag cgatcgacgt cgaagtcgac gcgggcttgg
      181 gatccgccgt ccagcagttc gaccttcgag accttgccga cctcaacgcc ggaggcgcga
      241 acgaactggc ccgcgcgaag gccactcgta ttggagaaga tcgccgaata accggtggtg
      301 cggtcgaacc gcatctgccc gaacaccacg atgagcatcg cggtgaacag cagcagcacc
      361 agcgagaaga tgccgagctt gacagcagtt ccggtgattt tcatgggttg atcgtgttct
      421 ccccgaactg gcggccccag acgtattcga tcccaggaat gatcagcgga atcggcgccg
      481 gcagaagcgg aatgaggcaa ggcggcagcc cggcgcaacc gaacggcgac gcaagctcga
      541 tgtggttgta cggcgcgatg gaggccccgg tgtccatcac caggtacggc gcgggccaga
      601 ggtcgcgggt gatcggctgc cagcagccgg gcctgccctc gggaccgcct cgcgcgttca
      661 cccgcggcag gttgtcggga tagacgtagg cgttacccgc gccgataagt tcggaagtgg
      721 tccgcaacga gtatccgttg ccgcccaacg acgcggcgac cttcggttcg acatcgtgga
      781 agttgcggat cgtgcagaac agcgcggggc tgtacgtgtc gagcagttgt gaggtgggca
      841 ccaggtcctc ggtgccgcgc acgagatagg gcccgctgcg ttcgaagatg tcgccgccgg
      901 tgttgccgaa gccgatcgag gccatcaacg cctgatcgat gttggcctgc tgctcgttga
      961 gcgtgcgcgc ggtcgtcacc gcgttctcca gcccgtcgaa cagatcgggc gccgcattgg
     1021 catacacgtc accgaggtcg gccaacaact ggttgtcgcg ccggatctgg ggcatctgcg
     1081 ggttgatgtc ggccaggatc tcgttgccgt gaatgatcga ctggccgaac cgatcaccga
     1141 gcccgttgag cgattccgca gtggcggaca aggtttggtt caacttgatc ggatcgacct
     1201 gctgagccac ctccaccacg gtctcgaaca acgtgttgaa ctcggtggtc accgacgtca
     1261 catcaatcac atcggaagtc gtaatacgtt gtggtgacgg gtctttcggc gacgagaagg
     1321 agatgtactt attgccgaac accgtggtgg cggtgatatc cgcattgacg ttcttcggga
     1381 tgagactgaa gtacttgggg ttcacgtcca ggatgacctt ggcccgcggc tgatcgccaa
     1441 cggtgatctc ctcgaccttg gcgaccctgc cgatctccac gccgttgtag gtgaccttcg
     1501 cccccgggtc catcgacagg cccgcgcgcg acgagatcat ggtcagctgt tcgcgatcca
     1561 ggttgtagcc gcgaaacaac acgtaaacca ggaccagcac gacgacggtc agcaacgtca
     1621 aaatcaaacc cgccaacttg aatggcggtg tccgaggctt gttcagcggt gcggtcatcg
     1681 gctacaccgt caggttgaag ttcgggtcga cgccgtagag cgccaacgca acaaacagga
     1741 ccacgagtgc caccgccacg agtgacgctc gcatcgctcg accgacggcc tcgccgacgc
     1801 cgaccggccc accgctggcg taatagccgt agtagcagtg gttgagcatc acgatgatcg
     1861 aaatgatgat cgcgtccacg aacgaccaga acacgtcctc gggccgcagg aacgtactga
     1921 agtagtgctc gtacgtgcca gtggattgcc cgtaaaacag tgtggtcgtc acctgaccgg
     1981 acaggaacga caagatcatc gccatcgcgt acagcgggat gatcaccaca aagccggcca
     2041 tgattcgcgt cgacaccaga tacgagatcg acttgatgcc catcacctcg agggcgtcga
     2101 tctcctcgct gatgcgcatg gcccccaact cagcagtggc gcctgccccc acgaccgcag
     2161 cgagagcgac acccgacacg agtggagcag cggagcgcac gttgatcatt gcggcgaaga
     2221 agccggtgaa cgcctccaca ccgatgttgc ccagcgacgc gaatccctgg atcgcgacca
     2281 gcgatgaacc ggacaaactg acgaagccga cgatcgcggc ggtaccgccg accacggcca
     2341 tcgcgccagt gcccatgccg atctcggcga tcagccgcag cgtctccttg cggtaatagc
     2401 gcagggcatg cccgatcgag cccatcgagg tgacgacgaa ccacgcgaca tggccgacgc
     2461 tgtcgaggcc acgtgcaggc gcaccggcga ctttctcggc gcgggcgact ccccgcggga
     2521 atcgagagcg caggacttgg gtcgttgaca tgtcagcgcc ccgttccgaa ccggacacca
     2581 atcgtggtca gcaccacgtt gaccgcgaac agcgcgatga cgcacagcac cagcgtctcg
     2641 ttgacagcgg tgccgacgcc cttggcgcca cccgacactg tgaggccgcg atagcagccc
     2701 accagcccgg cgatcaaacc aaagacggtg gctttgacca ccgaaatgag cacctcaggg
     2761 aggccagtga tcagcgtcag cgtcgagaca tacgcgccgg ccgagacatt ctgcagatac
     2821 acgccgaaga cgaagccacc gaccaggccg atggtgatca ccgctccgtt gagcagcagg
     2881 gcgacgaacg tcgaggcgac cacgcgggga acgaccaggc ggtggatcgg gtcgatgccg
     2941 agcacctcga gcgcgtcgat ctcctcacga atggtgcgtg cgccgaggtc ggcgcagatg
     3001 gccgtcgatc cggcgcccgc caccacgagc actgttacca atgggcccag ctgggtgacg
     3061 gcgccgatcg ccgcgcccgc gccggagaca tcggcggcgc cgaattcggc gagcaggatg
     3121 ttcagcagga agatcaggag cacggtcagc ggtgctgaga ccgcgagcgt gggcaggaac
     3181 gacacccgga acaagaacca ggactgcagg atgaactcgc gccactgaaa cggcggcctg
     3241 aataaggcct ttccggtgag tacacacatg cggacgaagc cgccgacagc cgtcagcgcg
     3301 ggcctggtct gatcgcggac gtagtcgacc acgccggtcg acgccgtcac cggtcgcctc
     3361 gcacgacgaa accgcgactg tcgtggcgca gcggcagacc ctgtcgcacg ccccctcctt
     3421 gccccaaccc ggtgtgtgtg accaccgtct ccttactagc gggtagtaag acagaccaca
     3481 ggactgtacc cgatgctgta cccgccgtac accgcatcgg ccggattgtg cccgtaaccg
     3541 ttagcaaacc cacccccagc ccttaattgt cttgtgcccc agcagaaacc gttggcggag
     3601 tggaactttc gccagcgtca atcaattctt gcgcaccgaa acgcgctcgc agttccgttt
     3661 tgaggacttt gccagcgggg ttgcgcggca gggcgtcgac gatctccagc gccttcggat
     3721 gcttgtaccg cgccagccgt tcggtgagga attcgtcgag gtcggccagg tcaagatcgg
     3781 tgtgaacggc agccacggcg acgggaacct cgccccactt ggggtcaggg cggccgatca
     3841 ccgccacttc gacgatggcg ggatgcccgg ccagggcgtt ctcgacctcg gcgcagtaga
     3901 tgttctcgcc gcccgagatg atcatgtcct tcttgcggtc gacgacccag acgtagccct
     3961 cgtcgtcctg gcggaccagg tcgcccgagt ggaaccaacc gccggcgaag gcctcggccg
     4021 tggctttcgg gttgttccaa tagcccgcca tcagcgtcgg cgcccgatac acgatctcgc
     4081 cgacctggcc gacgggaaca tcgttcatgt tctcgtcgac gacacgagcg gccaccgtgg
     4141 ggatcacctt gccgacagac ccgagcttgc gaattgcgtc ctgcgccaac aacatacacg
     4201 tgacgggcga catctcggtc tgtccgaacg cggcgtagat ctgggtgcca ggaaaggtct
     4261 cggccatgtc gcgcagcagc gtatccgacg ccggcgcggc accccacgac agcactcgca
     4321 gcaccaactt gcgtgggttg atccgctgct cggcgaccac cgcctgccac tgcgcgggca
     4381 ccaggaacat cgaggtgact tgctcggcct cgagcacgtc gagcagtgca ccggggtcga
     4441 aggccccgag cggatagagg acggtcggca gcccgagctg cagtggggtg atcatgttgc
     4501 cgatgccggc gatgtggaac agcggcaccc cgacgaaccc gacgtcccga ttgatgtcgg
     4561 cgccgatggt gaacaggttg gtcatcgcct ggccggcgag gttggtgtgg gtgagcaccg
     4621 cgcccttggg cctaccggtg gtgccggagg tgtacatgat cagcgcaggc gagtcgttgg
     4681 ggatgtcgat gggatccggg cctgcggcac cttcttcggc gatcaggtcg tcgtagccga
     4741 cgacctcgtc gtcggtgcca gcgcccgcga cgatcacggt cgcgagcgtc gggtcgatgt
     4801 cgcgtacggc ggtggcgacc ttggccagca cggcttcggt gaccaccacc ctggcctggc
     4861 agtcgctgac caggaaggcg atctctggcg gcgtcatccg gaagttcacc ggcacggcga
     4921 tggcaccgag tctggtggcc gcgaggaacg actcgatgaa ctcggtgcgg ttgagcatca
     4981 ggatcagcac gcggtcaccg aggctgacgc cgcgcctgct tagcgcactg gccagggaac
     5041 ccacccggcg atcgagatca gcccaggtcg tcgtgtgacc gagaaaccgc agcgcgacgg
     5101 cgttgggctg catcagtgcg tgcctggcga gctggttggc ccagttctgc ctgcgcgcca
     5161 gatacggctg cgcggtaggc ggcggctcgg tcaacggctg gtgctccctc gggcggtagg
     5221 ttgcgcgacg ttgatatttg atcaaacatg gtgttgccgt ggtcacacta tggccttggc
     5281 ggcccgggga caaccccgcg ccagacaacc ggagacagtc atgaacgcac ccgccaggcc
     5341 ccgcgctcag cggcgccggg cggtacgtcg cgagcagctc tccgacgagg tcgccgcccg
     5401 gttgcgcacc gacatcatga ccggcacgct gcggcccggg acgttcatcc ggctcgacga
     5461 gacggccgcc gcgctcgggg tcagcatcac gccggtgcgt gaggcgctgc ggaccctgcg
     5521 cggcgagggc atggtgcagc tggagccgca ccgcgggcat gtggtggtgc cgctgacgcg
     5581 gggcgacatc gaggacatct tctggctgca ggcgacgatc gccaaagagc tcgccgcgac
     5641 ggcggccgaa cggatcacgg atgcgcagat cgatgagctg gaacggctca atgagggcct
     5701 cgcggcggcc gtcgagagtg gcgatcccga ggaggtcgtg gcggcggagt tcgcgttcca
     5761 ccgggcgttc aaccgggcaa cgggccgaat caagctggcc tggttcctgt tgcacgtcgc
     5821 gcggtatctg ccggggcaga tctatgccag cgatgcggcg tggggtgcgg cggcggtggc
     5881 cggccaccgt gagctgattg cggcgttgcg caggcgtgac gtcgatacgg tcgtccggct
     5941 caccagcggg gagttcagcg acgcggcgca gcggttggtg gcgcggctcg acgagatcgg
     6001 gttgtggagc tgacggcagc tacgggctgg cggccagctg ctcggcgcgc gccttgaggt
     6061 tgtcggccag atagtccagc gtgtcgccga tcgccttctt caccatcggc ttggggatcg
     6121 gcagcttggt ctcgacgtcg aagtcgaccg tcagcagtga ggtcgccccg atcggcacca
     6181 cggagaactt ctgctcctgc ttctcgaaga actcgccctg ctgcagcacg gtgtagatct
     6241 ggttctccgc ggggtagtag accgcggtga tgaacgtgcc cgactggccc tggaccacga
     6301 catcgaggcg cagctggctc ggcctgccgt cctcatatcg ggccagcacc cagcagccct
     6361 tgatctcctc gttccacaat ggatacgact cgaaatcggc gacgatcgcc atgatcgatt
     6421 ccgcgggcgc ggcgacctca actgtcttgc ttacgagcgg catcggccga gcatatcgac
     6481 tccagcagcg accgaatgct cgccgggatg ggcacggggc gccgggtggt cctgtcgata
     6541 tagacatgca cccaatggcc gaccgcggca accggcccgt ccttttgaaa cagggccagc
     6601 cggtaggtga cgctgctgtt gcccaatcgg gccaccgcca gcccaacagt cagcgggtcg
     6661 gggaacttca actcggacga gtacttgcag cccgattccg cgaccacacc ctgccacggc
     6721 accgacagcg ggtcgatcgc gcagctcgtg ttgatccacg cattgatcgc ggtgtcgaac
     6781 agctcgtaat acaccgcatt gttgaggtga ccgaacatgt cgttgtcggt ccagcgggtc
     6841 agcaccggcc agtgcaccgg gaagtcgcgg ctggtgagcg attcctcgct agtcatggca
     6901 gcagtgtccc aggcacggca ggctggtcgt catgaggatc cgcggcgccg tgctcgagga
     6961 gatcggtagg ccccggccct acgccgaatc ccggccgctc tcgatcagcg agctcgacct
     7021 ggcgccgccc ggcgactccg agttgctggt tcgcatcgag actgcgggcc tctgtcattc
     7081 cgatctgtct gtggtcgacg gcaacagggt ccgcccggta ccgatgctgc tcgggcacga
     7141 ggcggcaggc atcgtcgagc aggccggctc cgccgtcgcc gacgtgcccg tcgggcagcg
     7201 catcgtcatg acgtttctgc cgagatgcgg ccagtgtgcg gcgtgcgcca ccaacgggct
     7261 gacgccttgc gaacccggca gcgccgccaa cggcgccggc acgctgatga ccggcgagcg
     7321 gcgcctgtcg cgcgacggcc aacccatcca tcaccacctc ggtgtctccg gcttcgccac
     7381 ctacgccgtc gtcgaccgtc gctccgtggt gccggtgccc gccgacgtgc cgccatccgt
     7441 cgcggcgctg ctgggctgtg ccgtcctcac cggtggcggg gccgtcatca acgccggacg
     7501 accacaagcc ggccaaaccg tcgtggtcgt cggactcggc ggcgttggga tggccgcggt
     7561 catcaccgcg ctggcgcacg acgacgtcac cgtcatcggc gtcgaccagc tcgccgacaa
     7621 gctcgacctg gcgcggtcga tgggcgtgca cgaggcctac acgcccgacg aggcacgcga
     7681 gcgcggcgtc aaggccgcgg tcgtgatcga ggccgtcggc cacccgctcg ccgtgcagac
     7741 cgcgatcgac ctcaccgcac ccggcggccg caccgtcacg gtcggcctac cgtcacccga
     7801 cgcacgaatc accgtgtcgc cgttgggttt tgtcgccgag ggccgctcgc tgatcggcag
     7861 ctacctcgga tcggccgttc cgtcgcacga catcccgcac ttcgtcgggc tgtggcgatc
     7921 gggccggctg ccggtcgaaa agctcgtctc gtcgacgatc aacctcgacg aaatcaacac
     7981 cggcatggat cagctcgccg acgggcacgc ggtccgccag ctcatcagct ttgactagtc
     8041 acggcctcgg tggtcgcgat accggcccgg cgcagcgccg ccaccacgag ggcgcgtagc
     8101 cggcggccga cctcgaactg cttgcccggc agcgtgcgcg ccaccatccg catgttgagc
     8161 gtgtcgacct cgatgctttc cacgcccatc agttgtggct cgtcgagcag cagatccggc
     8221 agcccgtcgt cttgcatcgc cgtcgtcgag acgtttcgca ggacgtcagt gaccttgttg
     8281 atgtcggctg ctgcaggcac cgggatatcg acgacggcgc gggcccagtc cttcgacatg
     8341 ttgagggact tcacaatctg accgttcggc acggtgaaga cctcaccgtc gctggtgcgc
     8401 agcttggtga cgcgcagcgt gacctcctcg acggttccga ccgcggcgcc gcctgcggtc
     8461 acggtcagct cgaccaaatc accgaacccg tattgctttt cggtgatcag gaagaatccg
     8521 ctgagcaggt cctgaacgat gcgctgggcg ccgaagccga gggcggcacc cagcaccgcc
     8581 gccggagcga ccagggaacc gacaggcagg cccagttgat tggcgacatc gatggaaacc
     8641 accacgtaga gcagtgcgat cgccaccgac gagataactg atgccacggc ttgccggtgc
     8701 ttgacgcgct ccgggcgcag gacggcgtct gcgccgtcgt cggcatcgag ccgcttgctg
     8761 atccgccccg ctatccatct gatcaaccgg gtggtgatga cggcgaacag caccaccaaa
     8821 acgatgtgca ggccgttagt gaggagccat tggctgaggt cgcgaagtgt cgtaggcata
     8881 cccgcggacg gcttcccact cccccggggg tttcaaacac gagcgaacaa cttgtatgat
     8941 gagttgcaaa cttgtcatat ctaatcctgg gagccttgat gtccgaccgc atccgccatc
     9001 tgaagctttc gcatgtcgtg ctgccgttgg aaacgccggt cagcgacgcg aaggtgctca
     9061 ccggacggca ggaaccgctg accgaaacgg tgcttttgtt cgtcgaggtc ctcaccgaag
     9121 agggccacga aggcaccggg ttctcctact ccaagcgcgc cggcggccct gcgcagtaca
     9181 cccatctcgc ggaaatcgcg gacgtcgcac ttggtcaaga cccctccgac atcgaccgga
     9241 tctaccaatc gctgctgtgg gccggcgcct cggtcggccg ctccggcgtc gccacccagg
     9301 cgatcgccgc gctcgacgtc gcgctgtggg acctcaaggc gcgccgcgcc ggcctgccgc
     9361 tggccaaact gattggcgcg caccgcgact cctgcccggt ctacaacacg tccggcggct
     9421 tcctgcaagc ttcggttgcc gagataaagg agaaggccac cgcgtcgctg gcgtccggca
     9481 tcggcggcat caaaatcaaa gtcggacagc cggattggaa gatcgacctg caccgcgtgg
     9541 ccgtgctgcg cgagcacctc ggcgagacac cgttcatggt ggacgccaac cagcagtggg
     9601 atcgcgccag ggcccgccgg atgtgtcgcg agctcgaggc gttcggcctg gtgtgggtcg
     9661 aggagcccct cgacgcatgg gacgccgtcg gccatgccga tctcagccga acctttgaca
     9721 cccctatcgc gacgggcgag atgctgacct cggtaagcga gcacatggcg ctgctcgacg
     9781 cggggtaccg aggaattgtg cagcccgacg cgccgcggat cggcgggatc acgccgttcc
     9841 tcaagttcgc gacgctggcc gcgcatgccg gcctggcgtt ggcgccgcac tatgcgatgg
     9901 agatccatct gcacctggcc gcgacgtatc ccaccgagcc gtgggtcgaa cacttcgaat
     9961 ggctcaaccc gctcttcaac gagcgcatcg agattcgcga cggccggatc tgggtgcccg
    10021 atcggccggg cctcggcttc acgctcagcg agcagatgcg ggcgctcact aaggaaacgg
    10081 ccgacttcgg ggcagcatga cgacgacact cgggcagcgc gtggtcgccg gtttgaagga
    10141 caagatcctc gccggggacc tgccacccgg gcacaagcta ccgtcggagg ccgagctcat
    10201 cgacgagtac ggggtgtcgc gaacggtggt ccgtgaggcg gtgacgcggc tacgggccga
    10261 ggggctggtc gaaacgttcc agggccgtgg ctcgttcgtg ctggcgatgc ccgagccgac
    10321 gtcgttcacc gtggaatccg cggccatccg gacccatcac gacgtcctcg acatgatcga
    10381 ctttcgcctc ggaatcgaat gcgaggcagc cgcattggcc gcggcgcggg cggacacgtc
    10441 cggcgccgag tcgatacggg ccgcgctcga cgccttcgtc gccgccgcgc cggaagacca
    10501 agtggaggcc gacttcggat ttcaccgggc catcgcggcc gccagtggca gccgcttcta
    10561 cctcgagctg ctcgac
//
LOCUS       DASRJA010000178         5075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2977613,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000178 DASRJA010000000
VERSION     DASRJA010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2977613"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1063)
                     /locus_tag="VFC01_08205"
     CDS             complement(143..1063)
                     /locus_tag="VFC01_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HZN79654.1"
                     /translation="MTTTQEALLTELEPVVEANLNRHLEMAKPWNPHDYVPWSRGRDF
                     ALLGGQDWTPADSQLDEVSKAAMIVNLLTEDNLPSYHREIATRFGRDGAWGQWVGQWT
                     AEEGRHAIAIRDYLLVTRGVDPVELEHGRMVHTAAGYDSGDKSPIEALAYVSFQELAT
                     RISHRNTGRASGCPIAEQLLTRIATDENLHMVFYRNLVNAALDIAPHATMKAIAREVI
                     NFQMPGSTMPGFAENALAIAKAGIYDLRSHLDDVVRPVLRFWGVFERENFDGEAARAR
                     DELAAFLDLVEEKASYYDQRREERLVTSAR"
     gene            1374..2387
                     /locus_tag="VFC01_08210"
     CDS             1374..2387
                     /locus_tag="VFC01_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN79655.1"
                     /translation="MKVPFTWKVTGWFVIGWSAEFPVGEVRPLRYFGEDLVAYRDEDG
                     ELHLLSGHCRHLGAHIGHGGKVVGDCVECPFHGWRWGPDGTNRYIPYQPDRPNKALRL
                     RVFPVREQYGCVFAWHQPDGKEPQWELPDLFGKFPQFATAPDAYYRPYPEFSSRAEHE
                     PVHPQIVAENGPDSAHFHYVHGATVTPVCLNWEAVDEEWRFLTGWPDARSDEPNEMAL
                     YIHSHFSGLGFAISAFEGSSNHRLIFACTPVEDGYSDMFYSIWWPRLPGDTSDVPPDK
                     VREQVERQFLGTVWEDLDIWRYQEYIERPALSKVDAKPYMAMRKWATQFYEVPPTNAV
                     ASA"
     gene            2384..2995
                     /locus_tag="VFC01_08215"
     CDS             2384..2995
                     /locus_tag="VFC01_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HZN79656.1"
                     /translation="MKPDLAEIAGPAHTAIVTQECQGAVVGLNAGLAMLAEEARREAV
                     PNIGRLLPAARDAGVKVVHCLVQRRPDGLGGNHNAKIFAIGSGVDIVPGSPGAQLLPE
                     LGPAPTDVVLYRSHGIGPMGGTDLDATLRNLGISTIVAVGVSVNVAITNLVMDAVNAA
                     YRVVVPRDAVAGIPADYATAIIDNTLSLLATITTTDDLIEAWK"
     gene            2992..3606
                     /locus_tag="VFC01_08220"
     CDS             2992..3606
                     /locus_tag="VFC01_08220"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN79657.1"
                     /translation="MTDTDSETTAFPEIKPVDAPPELGRFMAAMRRLQDIAVSTNPDG
                     AVWADAAERVEELCERLAVHEAPEGVAPAGRGPHLPGLGHPLMPPWMITESGADGVVM
                     HGHFSRFHVGGNMAVHGGVIPLFFDWHFGMIVSAAGRADSRTAYLHVDYRKVTPIEEP
                     LVSRGRIDTIDGRKAFVSATMTDSDGTVLSEASGLMIRLLPHQP"
     gene            3615..>5075
                     /locus_tag="VFC01_08225"
     CDS             3615..>5075
                     /locus_tag="VFC01_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HZN79658.1"
                     /translation="MSEVATQFTVPAAADTVAAAIGDREFLIQGDRRYTYAQVVARAD
                     RLAAYLHSRGLGAKTERSTLAGHEVGQDLLGIYAYNGPEYVEAMLGSWRARVAPFNVN
                     YRYVKNELHYLLADSGATALIYHAAFAPRVADVLPQLPHLRVLIQIADKSGNDLVHGA
                     VDYESIVRSGVPVLPPDDPSPDDLYVLYTGGTTGMPKGVLWRQHDIFMTSFGGRSLYT
                     GELANTYEDIAKRVADAPESRLMILPPLMHGAAQWAVMTAMTTGQSVVFSTVTDRFDA
                     DEVVRTIEREKIMAVTVVGDAMARPLAAAIERGADLSSLAVVANGGALLTPTAKQRLI
                     DVKPGLIVVDGVGSSETGAQMTHMSAPGAVATGKFNAGPDTFVAAEDLSTILEPGHDG
                     IGWLAQRGYVPLGYKGDEAKTAATFPVIDGVRYSVPGDRARHLADGAIELLGRDSVCI
                     NSGGEKIFVEEVETAVASHPAVADVVVSGRPSERWGQ"
BASE COUNT          911 a         1673 c         1596 g          895 t
ORIGIN      
        1 aagccggcgc caatcacgat cgcgtcgaag cgagcaggca tggtcacaag tcggcacctc
       61 aagatcccag ctatagcctg atactgtaac gattacagta tcagagcccg gcgccaagag
      121 cgatcgcgaa atctaccgtg atctaccgtg ccgaggtgac cagccgttct tcgcgacgct
      181 ggtcgtagta agaggccttt tcttccacta gatcgagaaa tgccgccagt tcgtctcgcg
      241 cgcgagctgc ttcaccgtcg aaattctcgc gctcgaatac tccccagaac cgcaacaccg
      301 gacgcacgac atcgtccaaa tgcgaacgga ggtcgtagat gccggccttg gcgatcgcta
      361 gagcattttc cgcgaagccc ggcatggtgc ttcccggcat ctggaagttg atcacttcgc
      421 gagcgatcgc cttcatcgtc gcgtgcggtg cgatatcaag cgcagcgttc accaggttcc
      481 gatagaagac catgtgcagg ttctcgtcgg ttgctatccg cgtgagcagt tgttcggcga
      541 taggacatcc agatgctcgg ccggtgttgc ggtgactgat ccgcgtcgcc agttcctgga
      601 aggacacata ggccagggcc tctatcggtg acttgtcacc cgagtcgtag ccggccgccg
      661 tgtgcaccat acgaccgtgc tcaagttcca cgggatcgac gcctcgggtg acgagaagat
      721 agtcgcggat cgcgatggcg tgacgcccct cctcggccgt ccactgaccg acccactggc
      781 cccaggcgcc atcacgcccg aaacgcgtcg cgatttcgcg atggtaggac ggcaggttgt
      841 cctcggtcag aaggttcacg atcatggcgg ctttggaaac ctcgtcgagc tgggaatccg
      901 ccggagtcca gtcctggcca cccagtaaag cgaagtcgcg cccacggctc catggaacat
      961 agtcgtgcgg attccagggc ttcgccatct caaggtgccg gttcaggttt gcctctacga
     1021 ctggttccag ctcagtgagc aatgcctctt gggttgtcgt catcgcgact ctccgtccct
     1081 tacctacggc tgcgtaacct acgctaccgt aggtttacgc aaaatcaagt attgattgac
     1141 gatcccaggg cccacacgtt gcgacgcggc ggcgagttca aattgactga ctgacatcct
     1201 gtttcgcgtc ggttgtaaaa gctggcggca gaccggccga tcgcgccatg gcgtttgcag
     1261 atggcgaagg cggccagcaa gatagtcgcg gcgtcgcagc ctgccgatat tgctctttgc
     1321 ggaaatctat actgtaatcg atttagtatc catcgactca agaggcggct gacttgaaag
     1381 tcccgttcac ctggaaggtc accggctggt tcgtgatcgg ctggtcggcg gagtttccgg
     1441 tcggtgaggt gcggccgctc cggtatttcg gcgaggatct ggtcgcctac cgcgacgagg
     1501 acggtgaact gcacctcttg tcgggccact gccggcacct tggcgcgcac atcggccatg
     1561 gcggcaaggt ggtcggcgac tgcgtcgagt gcccattcca cggctggcgc tggggacccg
     1621 acggcaccaa tcgctacatc ccctaccagc cggaccgccc caacaaggcg ctcaggctgc
     1681 gggtcttccc cgtccgggag caatacggct gtgtgttcgc ctggcatcag ccggacggca
     1741 aggaaccgca gtgggaattg cccgatctgt tcggtaaatt cccgcagttc gccacggccc
     1801 cggacgcgta ttaccgtccg tacccggagt tttcgagtcg cgctgaacat gagccggtgc
     1861 atccgcagat cgtcgctgag aacgggcccg acagcgcgca cttccactac gtgcacggcg
     1921 ccaccgtgac gccggtgtgc ctgaattggg aagccgtcga cgaggaatgg cggttcctca
     1981 ccggctggcc ggacgcgcgc agtgacgagc cgaacgagat ggcgctgtat atccacagcc
     2041 acttctccgg cctcggcttc gcgatcagtg cattcgaggg ttcctcgaac caccggctga
     2101 tcttcgcctg cacgccggtc gaggacggct attccgacat gttctattcg atctggtggc
     2161 cccgattgcc cggcgacacc tcggacgtgc cgcccgacaa ggttcgcgaa caggtcgagc
     2221 gccagttcct cggcacggtg tgggaagacc tggacatctg gcgctatcag gagtacatcg
     2281 aacgcccggc gctatccaag gtcgacgcga aaccctatat ggcgatgcgt aaatgggcga
     2341 cgcaattcta cgaggtgccc ccgaccaacg cggtcgcgtc cgcgtgaaac cagacctcgc
     2401 cgagatcgcc ggcccggcgc acaccgcgat cgtcacgcag gagtgccagg gcgcggtcgt
     2461 cgggctcaat gccgggcttg ccatgctcgc cgaggaagcg cgccgcgaag cggtgcccaa
     2521 catcgggcgt ctgctgcccg cagcccgcga cgccggggtg aaggtggtgc actgcctcgt
     2581 ccagcggcgg cctgacggac tcggcggcaa ccacaacgcg aagatcttcg ccattggaag
     2641 cggtgtcgac attgtgccgg gaagccccgg cgcacagctg ctgcccgaac tcggccctgc
     2701 gccaaccgat gtcgtgctgt atcggtccca cggcatcggg cccatgggtg gcaccgacct
     2761 cgacgcgacc ctgcgcaacc tcggcatctc aaccatcgtc gccgtcggcg tctcggtcaa
     2821 cgtggccatc accaacctcg tgatggacgc cgtgaacgcg gcgtatcgcg ttgtcgtgcc
     2881 gcgggatgcg gtcgccggca tccccgccga ttacgccacc gcgatcatcg acaacacgct
     2941 gtctctgttg gcgacgatca cgaccaccga cgacctcatc gaggcgtgga agtgaccgat
     3001 accgacagcg agacaaccgc gttccccgaa atcaagcctg tcgacgcacc gcccgagctg
     3061 ggccggttca tggcggccat gcgccgcttg caggacatcg cggtgtccac caaccccgac
     3121 ggcgcagtgt gggccgacgc cgccgagcgc gtcgaggaac tctgtgaacg gctggcagtc
     3181 catgaggcac ccgagggcgt cgctcccgcc ggtcggggcc cacacctgcc cggcctgggc
     3241 catccgctga tgccgccgtg gatgatcacc gaatcaggcg ccgacggtgt cgtcatgcac
     3301 gggcacttca gccgcttcca cgtcggtggc aacatggccg tccacggcgg ggtcatcccg
     3361 ctgttcttcg actggcactt cggcatgatc gtctcggccg cgggccgcgc cgacagccgg
     3421 accgcctacc tacacgtcga ctaccgtaag gtcacgccca tcgaggaacc actggtatcg
     3481 cgcggacgca tcgatacgat cgacggacgg aaggccttcg tctccgcgac gatgaccgac
     3541 tccgatggca ccgtgttgag tgaggccagt ggcctgatga tccggctgct gccacaccag
     3601 ccatgagagg cgacatgtcc gaggttgcaa cacaattcac cgtcccggcc gccgccgaca
     3661 ccgtcgcagc cgcgatcggc gaccgggaat tcctcatcca aggcgaccgc cgctatacat
     3721 atgcccaggt cgtcgcgcgg gccgaccggc tcgccgcata cctccactcc cgtggattgg
     3781 gcgccaagac cgaacgttca acgcttgccg gtcacgaagt cggccaagac ctactgggca
     3841 tctacgccta caacggacct gagtacgtcg aggcgatgct cggatcgtgg cgcgcccggg
     3901 tggcgccgtt caacgtcaac taccgctacg tcaagaacga gctgcactac ctgctcgccg
     3961 attcgggtgc caccgcgctg atctaccacg cagccttcgc gccgcgcgta gccgacgtgc
     4021 tgccccaact tccccacctt cgcgtgctca tccagatcgc cgataagtcc ggcaacgatc
     4081 tggtgcacgg tgccgtcgac tacgagtcga tcgtgcggtc gggcgtacct gtgttgccgc
     4141 ccgacgaccc gtcgcccgac gacctctacg tgttgtacac cggcggcacg acgggcatgc
     4201 ccaagggtgt gctgtggcgt cagcacgaca tattcatgac gtcgttcggc ggccgcagcc
     4261 tctacacggg cgagcttgcc aacacctacg aagacatcgc caaacgcgtt gcggatgcgc
     4321 cggaatcccg gctgatgatc ctgccgccgt tgatgcacgg tgcggcgcaa tgggcggtga
     4381 tgaccgcgat gacgaccggc cagtcggtgg tgttctcgac ggtcaccgac cggttcgacg
     4441 ccgacgaggt ggtccgcacg atcgagcgcg aaaagatcat ggcggtgacg gtcgtcggcg
     4501 acgcgatggc gcgaccactc gctgcggcga tcgaacgcgg cgccgacctc tcgtcgttgg
     4561 cagtcgtagc caatggcggc gcgctgctga ccccgacggc caaacaacgc ctcatcgacg
     4621 tcaagcccgg cctgatcgtc gtcgacggtg tggggtcctc ggagaccgga gcccagatga
     4681 cgcacatgtc ggctcccggg gcggtggcaa cgggcaagtt caacgcgggt cccgacacgt
     4741 tcgtcgcggc cgaggacctc agcacgatcc tcgagccggg tcacgacggg atcggctggc
     4801 tcgcgcagcg aggctatgtg ccgttgggct acaagggaga tgaggcgaag acagcggcga
     4861 cgttccctgt catcgacggc gtgcggtact cggtgccggg cgaccgcgcg cgtcatctcg
     4921 ccgacggcgc gatcgaactt ctcggtcgtg actcggtgtg catcaactcc ggcggcgaga
     4981 agatcttcgt cgaggaggtc gagactgcag tcgcgtcgca tcccgcggtc gccgatgtgg
     5041 tggtgtcggg tcggccgagc gagcggtggg gccaa
//
LOCUS       DASRJA010000179         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2410920,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000179 DASRJA010000000
VERSION     DASRJA010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2410920"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(601..1953)
                     /locus_tag="VFC01_08230"
     CDS             complement(601..1953)
                     /locus_tag="VFC01_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN79659.1"
                     /translation="MLGLGRRGTGMPQIVGIIGVLAFGFSVAPAAADTTRSVAFTDVT
                     VVPMDRERLLEHQTVLVQGDRIAAIGPVDSVRVPAGIERVDGRQKYLMPGIAEMHAHV
                     PGPDSPAAVEFMALYVLTGATTIRGMLGTSYQHELRRRIESGEILGPTLFMVAPPFSG
                     NSTLTADEARKKVREYAAAGYHVLKIFPPISREVYDAITSTAREVGIQYAGHVPPEVG
                     LRHAIEERQSVEHLDGYVEASRGNARAIEDLARATREAGIWNSATMDVWKTILGTRSL
                     DELHRRQELQYVPRPMIEQWAKETTEFARSSLRRRVLEQMGLRPSAMEVVALRDRILH
                     ALYRAGAGLILGADSPQVYSVPGFSLAHEMGAMVHAGLPIYAVLEAATKNASAFFRQE
                     AEFGTVEVGKRADLILVEGNPLEDIRNVHRQAGVMVRGRWLPKHEIQRRLDEIAQKWR
                     "
     gene            complement(2453..>2906)
                     /locus_tag="VFC01_08235"
     CDS             complement(2453..>2906)
                     /locus_tag="VFC01_08235"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZN79660.1"
                     /translation="GRHDAVPLVINLEQTPDRENRIMLGTDRDRFDLPKPEIHWHWRD
                     LDRHHLIRIRTIVADEIERNGIGRVVVDPDVPPDPNAHHHMGTTRMHHDPRRGVVSPD
                     GRVHGVSNLFVAGSSVFPTSGFANPTLTIVALALRLAEHLSDRHVRSN"
BASE COUNT          535 a          936 c          861 g          574 t
ORIGIN      
        1 gcgccgggag ggctggcgga tcaacgcgaa gaagacgtac cggctgtacc gaggcaaact
       61 tctcacggct gacccaattg cgtgacagcg acggctacag gcttccgccc gatatcgtgg
      121 tacgcctccg ccacgccggc ctatgatgtg ggcgttcgcc tggccgtcgc cgcattggtc
      181 atcgcggcca acaagccagc gatcaacccg tggcctagtc tgattgctca ctgctgttgg
      241 gattgtcttg ctaatgatgg acaggtcaaa cgatagcgaa agtcaaagct gacaatgtgc
      301 gccgacgcga cctgctccgg gtcggacatc ctcagttgtc tccgacagcc gaacgtcgcc
      361 agatcgagga aagaaaacgc cctacggttt agtcttcctg agggcgcgct tcttcggctg
      421 gaattccatc cgtaactgtc gttccgctca aaccagtcac caacaccgag accgtgttca
      481 ccgccacgac agacatagat cacgaatgcg cggtcagcag tttggatgtg ggtaggttcg
      541 cgtttcgttc gactgatctt cgccgttacc ttctcctgca gccggcaaca cattgaccgt
      601 ttaccgccat ttctgagcaa tttcgtccag cctccgctgg atttcgtgct tcggcaacca
      661 gcggccacgc accattaccc cggcctgccg gtgcacgttg cggatatcct caagcggatt
      721 gccctccacc agaattagat ccgctcgctt tcccacctcc accgtgccga actccgcctc
      781 ctgcctgaaa aacgcggatg catttttcgt cgccgcttcg agcaccgcgt atataggcaa
      841 gccggcgtgc accatcgccc ccatctcatg cgccaaggag aatcctggca cgctgtatac
      901 ctggggagaa tcggcgccga ggatcagacc ggcgcccgcg cgataaagcg cgtggagaat
      961 ccggtcgcgc agcgcgacga cctccatggc cgaggggcgc aagcccatct gctcgagcac
     1021 gcggcgcctg aggctactcc gggcaaactc tgtggtttct ttggcccact gctctatcat
     1081 cgggcgtggg acgtactgaa gctcttgtcg gcggtgaagc tcgtccagcg atcgcgtgcc
     1141 gaggatcgtc ttccatacat ccatcgtggc cgagttccag attccagcct cgcgcgtcgc
     1201 ccgggcgaga tcctcgatcg ccctcgcgtt gccgcggctg gcctcgacat aaccgtccag
     1261 atgctccacc gactgacgct cttcaatggc gtggcggagt cccacctccg ggggcacgtg
     1321 tccggcgtac tggattccca cctcgcgggc tgtactggtg atagcgtcgt acacctcgcg
     1381 tgagatcggc ggaaaaatct tgaggacatg gtagcccgcc gccgcatact cacgcacctt
     1441 cttgcgcgcc tcgtccgctg tcagagtgga gttgccgctg aacggcggcg cgaccatgaa
     1501 cagcgtaggc ccgagaatct caccgctctc gatccggcga cgaagttcgt gttgatacga
     1561 cgtgcccaac atgccgcgaa tggtcgttgc gccggtaagc acgtacagcg ccatgaactc
     1621 gactgcagca gggctatccg gacccggcac atgagcgtgc atctcagcga tgcccggcat
     1681 caggtacttc tgccgcccgt ccacccgctc aatcccagcg ggcacccgga ccgagtcaac
     1741 ggggccaatg gccgcgatcc ggtcgccttg gacgagcacc gtttggtgct cgagcagtcg
     1801 ctctcgatcc atcggcacca cagtgacgtc agtgaaagcg accgatctcg tggtgtcggc
     1861 cgcggccggt gctacgctga atccgaacgc aagcacgccg attatgccca cgatctgtgg
     1921 catccccgtc ccgcgacgac ccagtcccag catgcttact tcatacacgc ccatggcggc
     1981 aacaccagtc cgcgatccgt tccatcgcga aggttgcgtc tacgaggaga aggtcgatgg
     2041 gtgtgcattt gtgtcgcacc tccaattgcg cggatattac cgacgtacag cagtggttgg
     2101 agtaatagac agcgtcagtc gccgcatgca cgcgatcctg gttgattctc ctgtcacccc
     2161 ggacgtcttg cttcttacgc tcatgatcat cggcctggta tgtgctatcg cctgctactt
     2221 cgcggtcggt tccgaccagc cgcgccggcc gacgcgatga acgagggaca cgtctgccag
     2281 tcacatgcgc gctggatgaa ccgatccgcg tgccgcttta ttgccacgta tattgccacg
     2341 cggggggtcg ttcgcgggat ttctgggtag tttgtttcgt agcaacgaca acacaagaaa
     2401 gcgcccctcg ccgcgagcat cctgacctcc accgctcgat ggggatctag ccctagttcg
     2461 agcgcacgtg gcggtccgac aggtgctcgg caagccgcag tgcgagtgcc acgatggtca
     2521 gcgtggggtt ggcgaacccg ctcgttggaa acaccgagct gccggccaca aagaggtttg
     2581 agacgccgtg gacgcggcca tccgggctga cgaccccgcg cctgggatcg tggtgcatcc
     2641 gcgtggtgcc catgtggtgg tgagcgttcg gatccggtgg aacatcggga tcaaccacaa
     2701 cgcgcccgat cccgttgcgc tcgatctcgt cggcgacaat cgtgcgaatc cggatcagat
     2761 gatggcggtc gagatcgcgc cagtgccagt ggatctcggg cttcggcaag tcgaatcgat
     2821 cgcgatcggt tccgagcatg atccggttct cgcgatcagg agtctgctcg aggttgatta
     2881 ccagcgggac ggcgtcgtgg cggccg
//
LOCUS       DASRJA010000180         7009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2211263,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000180 DASRJA010000000
VERSION     DASRJA010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7009
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211263"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..2110)
                     /locus_tag="VFC01_08240"
     CDS             complement(221..2110)
                     /locus_tag="VFC01_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HZN79661.1"
                     /translation="MNTFRPSIDWSREFLDSTLWVLQVWVISAAVLLVVLFLVGRTTE
                     WGRQFWRVTGDYFKGRQSAPVWAVVGLLLLSAILVVRINVLLSYYANDLFSALQVTFQ
                     PDSARDTGIHGFWLTILVFAVLAVCYVARTLADLYLTQTFIMRWRIWLSRRFIDDWLG
                     DYAYFRSQFSRQPIDNPDQRIQQDIDVFTAGVGAEPNNPARTSDQLLLFGAVEAVVSV
                     FSFAAILWRLSGPLTLGSVTLPRALFWIVIFYVLAATIIAFVIGRPLIRLSFMNELLN
                     AGFRYALIRLRDASAAVGLYRGENAERHVLDRRLTSVMDNYGNWRNRMVLFTGWNLST
                     SQAIDPLPFIVQAPRLFAREISLGDIFQSATAFHTIHNSLSFFRDAYDSFASYRAAII
                     RLDGLLDENALARGFAHTTTPVSEDGQLEVDGLGVRIPDGQHLIRDLDFRLEPGNALL
                     ISGPSGIGKTVLLQSLAGLWPFVSGSVHLPCSREDAMFVPQLPYIPLGDLRAVVSYPH
                     QEGAVDDREIQQALVKVALPHLAIRLNDVRDWAKVLSVGEQQRIAFARVVLGKPKAVF
                     LDESTSAMDEGLELMLYELIRAEHPDAILVSVSHRGTVEQFHGRHLELIGDGEWRLDP
                     LPTRS"
     gene            complement(2107..3741)
                     /locus_tag="VFC01_08245"
     CDS             complement(2107..3741)
                     /locus_tag="VFC01_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZN79662.1"
                     /translation="MPNTDVLIAGAGPIGLTAAIELVRRGIDCRIVDPLLEPPQYAKA
                     VGVQPRTLEVFEGMGVLRRILDAATQMHGQIVYVNGEKTAQLDMTLPDDIPFGFIAIP
                     QYATERILREELAMRGVRVERGVRLTGFTQDDDGVTATFAGDTGEQTVRAGYLVGADG
                     AHSIVRKTLGLSFEGAAFEEQYMLGDVEVDWAMPRGYAVRAMHQTDGKTDDLLVCIPL
                     PGRGRYRMSMLVPDELSAAPTAGVAHGFEGDRKPELHHIQAVLDRLSPEPTTARNLRW
                     ASVFRISHRIVDAYGRGRVFVAGDAAHIHPPTGAQGMNTGIQDAHNLAWKLALAIDGH
                     AAAGLLDSYDAERRPVGEEVVGRTVRSAREGIGADSTDPDYVIRREAQLLINYAGSPI
                     VAGAAGPATSDTVGAGGRAPDATGLTRDAVTAPLRLFELLGRRGHTTLLYAPDWSVDV
                     ATFERAANAAVDAAHGRMDVYLIAAPDADVTATTLPLIRDAGGEFARAYQPSGPSAYV
                     VRPDGYLSFAAQSVDPDHLVAHLGATFAPPEGVPEG"
     gene            3762..5399
                     /locus_tag="VFC01_08250"
     CDS             3762..5399
                     /locus_tag="VFC01_08250"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HZN79663.1"
                     /translation="MSIDAPVTVNAGHLIARRLRASGIDTVFTLSGGHLFSIYDGCRA
                     ENIRLIDTRHEQTAAFAAEGWSKVTRVPGVAALTAGPGVTNGMSAMAAAQQNQSPLVV
                     LGGRAPAARWGQGSLQEIDHVPFVAPLTRFAATAQSADAVGGLIDDALRAAVGAPSGV
                     AFIDFPMDHVFSEAHDLGGPGALVDRPATADPDGNAVDTAVRLLADAQRPVIMAGTNV
                     WWGHAETALLNLAEVLRIPVLMNGMARGTVPADHDLAFSRARSKALGEADVALVVGVP
                     MDFRLGFGKVFGGQTKLIVADRAEPDRAHPREVAAGLYGDLRTSLSALATAGASDHEG
                     WLGELRAVESAGRAAEQAELDDDRTPLHPMRVYAELKPLLDRDAIVVIDAGDFGSYAG
                     RVIDSYVPGAWLDSGPFGCLGSGPGYALAAKLALPDRQVVLLQGDGAFGFSGMEWDTL
                     VRHGVHVVSVIGNNGIWALEKHPMEMLYGYSVVAELRPGTRYDEVVTTLGGHGELVSA
                     PAEVRPALERAFNVGVPAVVNVLTDPGVAYPRRSNLA"
     gene            5826..6377
                     /locus_tag="VFC01_08255"
     CDS             5826..6377
                     /locus_tag="VFC01_08255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN79664.1"
                     /translation="MRSRAAAFADAWHAITGRAGTTSSEERLYRLGTLRWPSGVAGTA
                     RLATDDDRSLLVDWVELFLGEAFDHPRDDDAGKAFVDHAHQVGDRFVLWDVDGIPVSM
                     AMLRAPAAAVSRIGPVFTPLTRRGHGYGSAVTAAAADLAHRSGTSDVVLFADLANPTS
                     NGIYQRIGFEAVCDSVRIDFRVP"
     gene            6382..>7009
                     /gene="secA2"
                     /locus_tag="VFC01_08260"
     CDS             6382..>7009
                     /gene="secA2"
                     /locus_tag="VFC01_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HZN79665.1"
                     /translation="MAKTKTRTSGRVSNRFWQLLGASTDKDQARSMAEVRASTEFEEK
                     AADLDDEQLTKAAKLLRLDDLAEADDIPQFLAIAREASERTTELKPFDVQLQGALRML
                     AGDVVEMATGEGKTLSGAIAAAGYALGGRSVHVITINDYLARRDAEWMGPLIEALGLT
                     VGWIRADSTADERREAYKCDITYASVNEIGFDVLRDQLVTDVDDLVSPN"
BASE COUNT         1183 a         2293 c         2415 g         1118 t
ORIGIN      
        1 tgaagtagtc gccggtgatg cgccagtact gcctgcccca cgtggtgtac ctggcgagga
       61 gcaccaggat caccatcgtg cacaccgccg cgatcaccca ggccttggcg atccatacca
      121 gcgatgtcca aagctcgtta ccccagtcca gcgtcggggt gaacatgttg tccattccgg
      181 caaaatacct cgataaactg cagtgagcgc cgccgagggc ctaactcctg gtcgggaggg
      241 ggtcgagccg ccactctccg tcgccgatta actccaggtg acggccgtgg aactgttcca
      301 ccgtgccccg gtgactcacg ctgaccaaaa tggcgtccgg gtgctcggcc cggatcagct
      361 cgtagagcat cagctccagg ccctcgtcca tcgccgacgt cgactcatcg aggaacaccg
      421 ctttcggctt acccagcacg acgcgggcga acgcgatcct ctgctgctcg ccaaccgaga
      481 gcaccttggc ccagtcccgc acgtcgttga gccggatggc caggtgcggg agggcgacct
      541 tgaccagcgc ctgctggatc tcgcggtcgt cgacggcacc ctcctgatgt gggtacgaca
      601 ccaccgcgcg caggtcaccg agcgggatat agggcagctg aggaacgaac atcgcgtcct
      661 cccggctgca gggcaggtgc acgctaccgg acacgaacgg ccacagcccg gccaggctct
      721 gcaacagaac ggtcttgccg atgcccgagg gaccgctgat cagcagcgcg ttgcccggct
      781 ccagccggaa gtcgaggtcg cggatcaggt gctggccgtc gggaatgcgg acgccgaggc
      841 cgtcgacctc gagctggccg tcctctgaca cgggcgtcgt cgtgtgcgcg aacccgcggg
      901 cgagggcgtt ctcgtcgagc aagccgtcca agcggatgat cgccgcccga tagctggcga
      961 acgagtcgta cgcgtcccga aagaaggaca gcgagttgtg gatggtgtga aacgcggtgg
     1021 ccgactggaa gatatcgccg agtgagattt cgcgcgcgaa cagccgcggc gcctgaacga
     1081 tgaatgggag gggatcgatc gcctggctcg tcgacaggtt ccagccggtg aacaacacca
     1141 tcctgttgcg ccagttgccg tagttgtcca tgaccgacgt gagacgacgg tcgagcacgt
     1201 ggcgttcggc gttttcgccc cggtacagcc ccacggcggc gcttgcgtcc ctcaaccgga
     1261 tcagtgcgta tcggaagccg gcgttgagaa gctcattcat gaagctcaac cggatcagcg
     1321 gcctgccgat gacgaacgcg atgatggtgg ccgcgagcac gtagaagatg acgatccaga
     1381 acagggccct gggcagggtg acactgccca gcgtcaacgg acccgagagc cgccagagga
     1441 tcgcggcgaa ggaaaagacg gatacgacgg cctcgacggc gccgaacagc aacagctgat
     1501 cggaggtgcg ggccgggttg ttcggttcgg caccgacacc ggcggtgaag acgtcgatgt
     1561 cctgctggat gcgttggtcc gggttgtcga tcggctgccg cgagaactgg ctgcggaagt
     1621 aggcatagtc gccgagccag tcgtcgatga agcgacggct caaccagatt cgccatcgca
     1681 tgatgaacgt ttgcgtcaag tacaagtcgg ccagcgtgcg cgccacatag cagacggcca
     1741 ggaccgcgaa gaccaggatg gtcaaccaaa acccgtggat gccggtgtct ctcgccgaat
     1801 ccggctggaa ggtgacttga agcgccgaga acagatcgtt ggcgtagtag ctcaagagca
     1861 cgttgatgcg gacaacgagg atcgccgaca acagcaacag cccgaccacg gcccagaccg
     1921 gcgcgctctg cctgcccttg aagtagtcgc cggtgacgcg ccagaactgg cgtccccact
     1981 cggtcgtccg cccgacgagg aagagcacca cgagcagcac ggcagcgctg atcacccata
     2041 cctgcagcac ccacagggtc gagtcgagga attcgcggct ccagtcgatc gacggtctga
     2101 atgtgttcat ccttcaggca ccccctcggg cggggcgaac gtcgcaccca ggtgagccac
     2161 caggtggtcc gggtcgacgc tctgagcggc gaaactcagg tagccgtcag gcctcacgac
     2221 gtatgccgac gggccggaag gctggtaggc cctggcgaat tcgccaccgg cgtcgcggat
     2281 cagaggcagc gtggtggcgg tgacgtccgc gtccggcgcg gcgatcagat acacgtccat
     2341 ccggccatgg gcggcgtcga cggcggcgtt cgcggcccgc tcgaaggtcg cgacatcgac
     2401 ggaccagtcc ggcgcataca acagcgtggt gtgtccgcgc ctgcccagca gctcgaagag
     2461 cctcagcggc gccgtcacgg cgtcgcgggt caggcccgtc gcgtccgggg cacggccgcc
     2521 ggcgccgaca gtgtccgatg tcgccgggcc agcggctccg gcgacaatcg ggctgcccgc
     2581 atagttgatc agcagctggg cctcgcggcg gatcacatag tcggggtcgg tcgagtcggc
     2641 accgatgcct tcgcgggcgc tgcgcacggt gcgcccgacg acctcttcac cgacgggcct
     2701 gcgttcggcg tcgtaactgt cgagcagccc ggccgcggcg tgcccgtcaa tggccagcgc
     2761 cagcttccac gccaggttgt gagcgtcctg aataccggtg ttcatccctt gtgcgccggt
     2821 cggcgggtgg atgtgcgcag cgtcgcccgc gacgaacacg cggccccgtc cgtaggcgtc
     2881 gacgatgcgg tggctgatgc gaaacactga ggcccatcgc aggtttcgcg ctgtcgtcgg
     2941 ctcgggggaa agccggtcca gcacggcctg gatgtggtgc agctcgggct tgcggtcgcc
     3001 ctcgaaacca tgcgccacgc ccgccgtcgg cgcggcactc agctcgtcgg gcacgagcat
     3061 cgacatgcga tagcggcccc ggcccggcag ggggatacac accagcaggt cgtcggtctt
     3121 gccgtcggtc tggtgcatcg cccggaccgc gtagccgcga ggcatcgccc agtccacttc
     3181 gacgtcgccg agcatgtact gctcctcgaa cgccgcgcct tcgaaggaca ggccgagcgt
     3241 cttgcgcacg atgctgtgtg cgccgtcggc gccaacgaga tagccggccc gcacggtctg
     3301 ctcgcccgta tcaccggcga aggtggcggt cacgccgtcg tcgtcctggg tgaacccggt
     3361 gagccgcact ccacgttcga cgcggacgcc gcgcatcgcg agttcttcgc gcaggatccg
     3421 ctcggtggca tattgcggaa tcgcgatgaa gccgaacgga atgtcgtcgg gcaatgtcat
     3481 gtcgagctgc gcggtttttt cgccgttgac gtagacgatc tggccgtgca tctgggtcgc
     3541 ggcgtcgagg atgcggcgca gcacccccat gccctcgaac acctcgagcg tgcggggttg
     3601 cacaccaacg gctttcgcgt actggggcgg ctcgagcaac gggtcgacga tgcgacagtc
     3661 gatgccgcgg cggacgagtt cgatggctgc ggtcaggccg atgggacccg cgccggcgat
     3721 gaggacatct gtgttgggca ccgcgtgatt atgctgccgc catgagcatc gacgctcccg
     3781 ttactgtcaa cgccggacat ctgatcgccc gacggctgcg cgccagcggc atcgacacgg
     3841 tcttcacgct ctccggcggg catttgttct ccatctacga cggctgccgc gccgagaaca
     3901 tccggctgat cgacacccgc cacgagcaga ccgcggcgtt cgcggccgaa ggctggtcga
     3961 aggtgaccag ggtgcccggc gtcgccgcgc tcaccgcggg tccgggtgtc accaacggga
     4021 tgagcgcgat ggccgccgcg cagcagaacc agtctccgct ggtggtgctc ggcggtcggg
     4081 ctcccgccgc gcgctggggg caggggtcgc tgcaggagat cgaccatgtg ccgttcgtgg
     4141 caccactgac gcggttcgcc gcgacggcgc agtcggcgga tgccgtgggc gggttgatcg
     4201 acgatgccct tcgggccgca gtcggcgcgc cgtccggcgt cgcgttcatc gacttcccga
     4261 tggatcacgt gttcagcgag gcgcatgacc tcggcggccc gggcgcgctc gtcgatcgtc
     4321 cggccaccgc cgaccccgat ggcaacgccg tggacaccgc cgtgcggctg ctggccgacg
     4381 cgcagcgacc ggtgatcatg gcgggcacca acgtgtggtg gggacacgcc gagacggcgc
     4441 tgctgaatct ggccgaggtg ctgcgcatcc cggtgttgat gaacggaatg gctcgcggca
     4501 cggtgcccgc cgaccacgac ttggcgttct ctcgcgcgcg gtcaaaagcg ttgggggagg
     4561 ccgatgttgc gctcgtggtc ggggtgccga tggacttccg gctcggcttc ggcaaggtgt
     4621 tcggcgggca gaccaaactg atcgtcgccg accgcgccga acccgaccgt gcgcatccgc
     4681 gcgaggtcgc cgcggggctc tacggcgacc tgaggacctc tttgtcggcg ctagcgacgg
     4741 cgggtgcatc cgaccacgaa gggtggctcg gcgagctgcg ggcggtcgag agcgcagggc
     4801 gggcagccga gcaggcggag ttggacgacg accgcacgcc tctgcacccg atgcgggtct
     4861 acgccgagct caagccgctg ctagaccgcg acgcgatcgt cgtgatcgac gccggtgact
     4921 tcggttccta cgccggacgg gtcatcgaca gttacgtgcc tggcgcatgg ctggacagcg
     4981 ggcccttcgg ctgcctgggc tccggccccg gctatgcact tgccgcgaag cttgccctgc
     5041 ccgatcgtca ggtggtgctg ttgcagggcg acggcgcatt cgggttttcc ggcatggagt
     5101 gggacacgtt ggtgcgtcac ggcgttcacg tcgtttcggt gatcggcaac aacggcatct
     5161 gggcgctgga gaagcatccg atggagatgc tgtacggcta ctccgtggtg gccgaattgc
     5221 ggcccggcac gcgatacgac gaggttgtca cgacgctcgg cgggcacggg gaactggtgt
     5281 cggcgccggc ggaggttcgg cccgcgctgg agcgtgcgtt caatgtgggc gtgcccgccg
     5341 tggtgaacgt gctcaccgac cccggcgtcg catacccccg ccgctcgaac ctggcctagc
     5401 aaaacagccg cgtgggccca cacgtgagta ggggacgtgc gtgttccccc gttgcacgcg
     5461 gggggtgccc ccagtacgcg acacgccgag gaaatccgtc caaacgcgca cgctcgcggc
     5521 cgtaaatcgg tggacgcgtt ggacattggc gcggatcctg agcatgtgga ggtaagactg
     5581 catgactccg tacgacgagt ttcgcgcgat cgcggaaccg ctgtatcggc gcgacccagt
     5641 cgtgtacacc gtcgagctga cactgctggc agcgaacaag tttccggacg acacctggct
     5701 gttcacggtg tgggacaacg gcgtacccgt cggcgccgcc ttacaaacgc cgccataccc
     5761 cctggggtgc aacggtattc ccacggacgc gatggattca ctcaccaccg acctggctag
     5821 gcggagtgcg gtcccgtgct gccgcgttcg ccgacgcatg gcacgcaatc acaggacggg
     5881 ccggaacaac aagcagcgaa gagcggctct accgattggg cacgttgcgt tggcccagcg
     5941 gtgtggccgg aacagcgcga ctcgccaccg acgacgaccg cagcttgctc gtggattggg
     6001 tggagctgtt cttgggcgag gcgttcgatc atccccgcga cgacgacgca ggcaaggcct
     6061 tcgtcgacca cgcgcaccag gtcggcgacc ggtttgtgct gtgggacgtc gatggcattc
     6121 cggtcagcat ggccatgctg cgcgcgccgg ccgcagcggt ctcgcggatc gggccggtgt
     6181 tcacgccgct gacccggcgt ggccatggct acggctcggc cgtcaccgcc gcggccgccg
     6241 atctggcgca ccgcagcggc acctcggacg tcgtgctgtt cgccgatctc gccaatccga
     6301 catcgaacgg catctatcag cgcatcggct tcgaagcggt gtgcgattcg gtgcgaatcg
     6361 acttccgcgt accgtaaatg tgtggcgaag acgaaaaccc gcacctcagg tcgtgtcagc
     6421 aaccggttct ggcagctgct cggcgccagc acggacaagg atcaggcccg ctcgatggca
     6481 gaggtccgcg cgtcgaccga gttcgaagag aaggccgccg acctcgacga cgagcaactg
     6541 accaaggccg ccaaactgct gcgccttgac gacctggccg aggcggacga catcccgcag
     6601 ttcctggcga tcgcccgtga ggcgtcggag cggaccaccg agctgaagcc gttcgacgtc
     6661 cagctgcagg gcgcgcttcg gatgctggca ggcgacgtgg tcgagatggc caccggtgag
     6721 ggcaagacgc tgtctggtgc gatcgccgcg gccggctatg cgctcggcgg gcgcagcgtc
     6781 catgtcatca ccatcaacga ctacctggcc cgccgcgacg ccgaatggat gggccccctc
     6841 atcgaagccc tgggcctgac ggtcggctgg atcagggccg actcgacggc cgacgaacgg
     6901 cgcgaggcct acaagtgcga catcacctat gcctcggtca acgagatcgg cttcgacgtg
     6961 ttgcgcgacc agttggtcac cgacgtcgac gatctggtgt cgcccaacc
//
LOCUS       DASRJA010000181         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_154197,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000181 DASRJA010000000
VERSION     DASRJA010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154197"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFC01_08265"
     CDS             complement(<1..193)
                     /locus_tag="VFC01_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HZN79666.1"
                     /translation="MHAIEVTETGGPEVLTYIDKPRPSPGPGEVLIKAEAIGVNFVDT
                     YFRSGAYPHTLPFVLGAEVG"
     gene            374..1987
                     /gene="mdlC"
                     /locus_tag="VFC01_08270"
     CDS             374..1987
                     /gene="mdlC"
                     /locus_tag="VFC01_08270"
                     /EC_number="4.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoylformate decarboxylase"
                     /protein_id="HZN79667.1"
                     /translation="MADQKTVHEVTYDLLRSLGLTTVFGNPGSTEETFLKNFPDDFTY
                     VLGLQEASVMAMADGFAQSTGKPALVNVHTGAGTGNAMGSLVAAYRGNTPLIVTAGQQ
                     AREMVLCEPYLTNSQETLLPQPWVKWAYQPARAEDVPAAFMRAYAMALQPPAGPVFLS
                     IPLDDWDKPALGPAVVRTVSHRVGPDAERLRTFADRINRAQRPMLVFGPEVDRSGAWD
                     AALAFAEKVGAPVYASPLPDRASFPENHRLYQGPLPMTIAGAEKTLRGHDLAIVIGAQ
                     VFRYYPYVAGDYLPEGTDLLQITNDPALAAAAPVGDSLISDSLLALEQLVGMVDDHSD
                     RTVPAAPVRNPAVDWSASPPLAPDAVWSTLASVKPAESPLVTESTSTMAQQMHWLPTT
                     RPASFFATASGGIGWGLPAAVGLALGDRARGVKRTVVATIGDGSCQYSVQAIYTAAQH
                     RLPVVFVVMRNGEYSILKSFAELEETPGVPGLDLPGLDIASLATGFGCRTVNVDNCEK
                     LAREFTNALDADGPTVIVVPTKPQPAHLG"
     gene            2019..>2692
                     /locus_tag="VFC01_08275"
     CDS             2019..>2692
                     /locus_tag="VFC01_08275"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZN79668.1"
                     /translation="MTEIVVDPSVTTGPIVGSTKIYRDGVPFRRVSLSNGEYLDLYDT
                     SGPYTDPHALIDLHKGLPSRPSVVRDRGTQLQRARAGEITAEMAFIAAREGVPAELVR
                     YEVAVGRAVIPANHNHPETEPMIIGKAFAVKINANIGNSAVTSSIAEEVDKMVWATRW
                     GADTIMDLSTGRNIHETREWILRNSAVPIGTVPIYQALEKVGGRPEDLTWEIYRDTLI
                     EQAEQGV"
BASE COUNT          510 a          873 c          840 g          469 t
ORIGIN      
        1 cgcctacctc cgcgccgagc acaaacggaa gtgtgtgcgg ataggcaccc gaccggaagt
       61 aggtgtcgac gaaattcacg ccgatcgctt cggccttgat caacacctcg cccggcccgg
      121 gcgagggccg gggcttgtcg atgtacgtca agacctcggg tccaccggtt tcggtgactt
      181 cgatggcgtg catggtcacg attccaacct gacgctcagc cctttgtcga tgaaacaaac
      241 gacaaccgcc gaaccctcac gttgtcgccg agaacatcga cggtgtcaaa cgcggccacg
      301 agagtaatga cagcgctcgc ctgacgacga cgcgatgacc atgctcaact acacgaaggg
      361 aattggcggg acgatggccg atcagaaaac tgtccacgag gtgacctacg acctgcttcg
      421 ctcgctggga ctgacaaccg tcttcggcaa ccccggctcg accgaggaaa cgtttctgaa
      481 gaacttcccc gacgacttca cctacgtcct cggcctgcag gaggcgtcgg tgatggcgat
      541 ggccgacgga ttcgcacagt cgaccggcaa gcctgcattg gtcaatgtgc acaccggagc
      601 aggcaccggc aatgcgatgg gcagtctggt ggccgcctac agaggcaaca cccccctcat
      661 cgtcacagcg ggtcagcaag cccgcgaaat ggtcttgtgt gaaccatatt tgaccaacag
      721 ccaggagacc ctgttgcctc agccgtgggt gaaatgggcc tatcaacccg ccagagctga
      781 agacgttccc gcggcgttca tgcgggccta cgcgatggcc cttcagccgc cggcgggccc
      841 agtcttcttg tccatcccgc ttgacgactg ggacaagccc gcgttgggac cggccgtagt
      901 gagaacggtc agtcatcggg tgggccccga tgccgagcgc ctgcgcacct tcgccgaccg
      961 tatcaaccgc gcacagcggc ccatgctggt cttcggcccg gaagtcgatc gcagcggcgc
     1021 gtgggacgcc gccttggcat tcgccgagaa ggtcggcgcg ccggtttacg ccagccccct
     1081 tccagacagg gcgtcctttc ctgagaacca ccgcctttat cagggtccgc tgccgatgac
     1141 catcgccggc gccgaaaaga ccctgcgcgg tcacgatctg gcgatcgtca tcggtgcaca
     1201 ggtcttccgc tactacccct atgtggccgg cgactatctg cccgagggca ccgatctgct
     1261 gcagatcacc aacgatccgg cgcttgccgc ggccgcaccg gtcggtgaca gcctgatcag
     1321 cgactcactg ttggctctgg aacaactcgt gggcatggtc gatgaccaca gcgatcggac
     1381 agtgcctgcc gcgcctgtcc gcaaccccgc agtcgattgg tcggcgtccc cgccgctcgc
     1441 cccagacgcg gtgtggtcga ccctggcttc ggtgaaaccc gcggaatcgc cattggtgac
     1501 cgagtccacc tcgacgatgg cccagcaaat gcactggctg cccaccaccc gcccggcttc
     1561 cttcttcgcg accgccagcg gtggaatcgg ctggggactg cctgccgcgg ttggcctggc
     1621 gctgggagac cgcgcccgtg gagtcaagcg gacggtggtg gcgaccatcg gcgacgggtc
     1681 atgccagtac tcggtgcagg cgatctatac ggcggcccaa caccggctgc cggtcgtgtt
     1741 cgtcgtcatg cgcaacgggg agtactcgat tctcaagtcg ttcgcggagc tcgaggaaac
     1801 accgggcgtt ccaggcctgg accttcctgg acttgacatc gcctcccttg caacagggtt
     1861 cggatgccgg acggtgaatg tcgacaactg cgaaaaactc gcgcgcgagt tcacgaacgc
     1921 gttggatgcc gacggtccaa ccgtgatcgt ggtgccgacc aaaccgcaac cggcacatct
     1981 gggctgacgc ggatctcgta ccgataggga gttttgtgat gactgagatc gttgtcgatc
     2041 cgtctgtgac cacgggaccg attgtgggta gcacgaagat ctaccgcgac ggggtgccgt
     2101 ttcggagggt gagcctctcc aatggcgagt acctcgatct gtatgacacc tcgggcccgt
     2161 atacggatcc gcatgcattg atcgatctgc acaagggcct gccctcgcgc ccgagtgtgg
     2221 tgcgcgatag aggcacgcag ctacagcggg cacgggcagg cgagatcacc gcggagatgg
     2281 cgttcatcgc cgcgcgtgaa ggtgtacctg ccgagctggt gcgctacgag gtggccgtcg
     2341 gtcgcgcggt gatccccgcc aaccacaacc accccgagac cgaaccgatg atcatcggca
     2401 aggcgttcgc ggtgaaaatc aatgccaaca tcggcaattc ggctgtcacg agctccatcg
     2461 ccgaggaggt cgacaagatg gtgtgggcca cccgctgggg cgccgacacc atcatggatc
     2521 tgtcgactgg ccgcaacatc cacgagacgc gcgagtggat tctgcgcaac tccgcggtgc
     2581 cgatcggcac cgtcccgatc tatcaggctc tcgagaaggt cggcggccgg cccgaggatc
     2641 ttacctggga gatttaccgc gacacgctca tcgagcaggc agagcagggt gt
//
LOCUS       DASRJA010000182         5307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3256177,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000182 DASRJA010000000
VERSION     DASRJA010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3256177"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4117
                     /locus_tag="VFC01_08280"
     CDS             <1..4117
                     /locus_tag="VFC01_08280"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZN79669.1"
                     /translation="ARFDDGGNVVEELDDGTLNRDGLDLASGIEVFEFVNGDLNHIRN
                     LSYPDLTPGPTTPGTHQFLQASDLYRLPSQDERTTGNVNHDGTANTDAATGIETVLTP
                     VGWAQTQAAADNGFKVAGDATDTEDQFAPIVKATEEAFVVAWQTAGATPGSVGVRMSM
                     YDPLGQPTLVDGGPGTTFSVTNNAAAGVAPAISGIGAGAVAAYVTADAGSLVVQAFDV
                     DGVPVGATLTVDAGTAAISDVCIAGQAVDGAVIEDQFAVVYVQDNGDTDTDYGNIVLQ
                     RYGVAVDANDVAQQPVALGRDGAANVGSDAAAQIEVDTGAGLETAIGRDPQAVGLHDG
                     QLAVVWVELSDTGSETVKGVVIEPNGNQVLQIDLTDMLPAAGIVEGTRPILAGAGEGD
                     ILVSWLQADPAGGFVVMAAIYKAAGADAWTVPEAPVALQHFDSEPQEFQVSVVGEVDV
                     SLIVTWREDGSGSNDDVHGQRFDTAGNTLGNEFDVTGNRSGDPEHQVAGDVSTAAGML
                     DGRFVVVYTEDDGNGEVDVEARMFDTRSAEDPNIGRDAGGPRGFEVGTVFDDIIDGRD
                     REDELHGGIGNDVLIGGVNDDALFGGAGQDTLVGGTGTDNLAGGEGNDLLMGGHGRDY
                     ISGGAGTDTISYRGEARAVTINLAAGTVHSDAAHNAVVLPDPATTINNLPADALTDAN
                     LEDLIGQIVEVDHEAEIFEFQSAADIENAEGGLGNDTIIGNDGDNVLTGGKGNDSLDG
                     GAGTDTALFSGAADEYRVTSNADGSFTVEDLLANRDGKDIIRNIELLQFADLAAAPDA
                     VAEEPPAPPIAVGNEVTASESDAEGGVIINVLANDTGADLRVAQINGVTITTQSPVQI
                     ASGFVQLREDGQLLFTANLNFSGVAAFAYTIANTAGLLATANVTVNVTPVNDAPTAVA
                     LSNLHVDEHVAGAVIGTLSAVDPDQPDTHTFTVSDQRFEVVGTTLKLKSGVSLDFATA
                     HTVDVTVTATDSGLLTTSQSFTIDVIGGNAAPTSITPDAAMVAENALLGTTAVSGLAA
                     VDPNVGDRAGFYLVNDVGGRFVMIGDTIIVANPTLLDYEAGSTHTIEVKAVDTGGLYK
                     TQSVTVTLGNLVGGADNPINGTAGKNAINGTSGADRINGLGGEDRMQGGAGDDILDGG
                     AGKDRLAGGAGNDTYVVDQTDDRVEEDRNQGTDTVVTSLDSFGLGDDVENLVYTGTRS
                     FTGTGNDDANTILGGSGADNLRGGRGDDTLVGRGGNDTLDGGDGHDALHGGDGNDTLT
                     GGAGNDILEGGAGSDILNGGVDNDTFVFRPGFGNDTIKEFGDSTNNQDVLDLSMSGYA
                     TFAALQAAGALAQVNADVVVTLNPLDPITSDKITLKTVNLSTLDATDFKFG"
     gene            4226..>5307
                     /locus_tag="VFC01_08285"
     CDS             4226..>5307
                     /locus_tag="VFC01_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZN79670.1"
                     /translation="MGRFFGAIRTLGEYCVVAGRPEKKKSEVRLAAGALGSVVITLAV
                     VSGVVNILALTGSFYMLQVYDRVLSSRSMATLLALSLLAVGLYLFQGGLEVIRSQILV
                     RLAGRLDRQLTKQAMTAAMRLRAYVGAQGSPLQPIRDVDAVRTYLSGQGPVAILDMPW
                     MPLYLAFVFILHPMLGWITMFGAAFLLSVAFLTERMMQTPGEVLASASKQRWAIAEAC
                     EQNSDALHAMGFGHRFLRRFQAVNNQHLDANERLSDVVGGLSSVSRVFRMMLQSALLG
                     IGAYLTIKGEMSAGAIIACSIAASRALAPIEVAIGNWRVFVAARQACARLDAVLANLP
                     EKSRPLMLPAPTHALSVESISVMVPGS"
BASE COUNT          935 a         1743 c         1780 g          849 t
ORIGIN      
        1 agctcgtttc gacgatggcg gcaacgtggt cgaggagctg gacgacggca cgctcaaccg
       61 cgatggcctc gatctggcgt ctggcatcga ggtgttcgag ttcgtcaatg gcgacctcaa
      121 ccacatccgc aatctgtcct atccggacct gacgcccggg ccgaccacgc cgggcacgca
      181 tcagttcctg caggcgtcag atctgtatcg cctgccctcg caggatgagc gcacaaccgg
      241 caacgtcaat cacgacggca ccgccaacac ggacgcggca accggcatcg agacggtgct
      301 gaccccggtc ggctgggcac agacgcaggc cgccgccgac aacggcttca aggtggcggg
      361 cgatgcaacc gacaccgagg accagttcgc gccgatcgtg aaggccaccg aggaggcgtt
      421 cgtcgttgcc tggcagaccg ccggcgctac cccgggttcg gtcggcgtgc gcatgtcgat
      481 gtacgacccc ctcggtcagc cgacgctggt tgatggcggc cccggcacga cgttcagcgt
      541 gacgaacaat gccgccgccg gcgtcgcccc cgccatcagc ggcatcggcg ccggggcggt
      601 ggcggcctat gtgacggcag atgcgggctc gctggtcgtg caggcctttg atgtcgacgg
      661 cgtgcctgtc ggcgccaccc tgaccgttga tgcgggcacg gccgccatca gcgacgtgtg
      721 cattgccggc caggccgtcg acggcgcggt gatcgaagac cagttcgcgg tcgtctatgt
      781 gcaggacaac ggcgacaccg acacggacta cggcaacatc gtcctgcagc gctacggcgt
      841 ggccgtcgat gccaacgacg tcgcccagca gccggtggcg ctcggccgcg acggtgcagc
      901 caacgtcggc agcgacgctg ccgcacagat cgaggtcgac accggcgcgg ggctcgagac
      961 ggccatcggc cgcgacccgc aggccgtcgg cctgcatgac ggccagctcg ctgtggtctg
     1021 ggtcgagcta agcgacaccg gcagcgagac cgtcaagggc gtggtgatcg aacccaacgg
     1081 caaccaggta ctgcagatcg atctgaccga catgctgccc gcagcgggca tcgtggaagg
     1141 tacccggccg atcctggccg gcgccggtga gggcgacatc ctggtcagct ggctgcaggc
     1201 cgatccagcc ggcggcttcg tcgtgatggc cgccatctac aaggcggccg gcgccgatgc
     1261 atggacggtg ccggaagcgc cggtggcgct gcagcacttc gactccgagc cccaggagtt
     1321 ccaggtttcg gtggtgggcg aggtggatgt ctcgctgatc gtcacgtggc gcgaggacgg
     1381 cagcggcagc aatgatgacg tgcacggcca gcgcttcgac acggccggca ataccctcgg
     1441 caacgagttc gacgtgaccg gcaaccgctc gggcgacccc gagcatcagg tagcgggcga
     1501 cgtctcgacg gcggccggca tgctggatgg ccgattcgtg gtggtctaca cggaggacga
     1561 cggcaacggc gaggtcgacg tcgaggcgcg catgttcgac acgcgcagcg ccgaggatcc
     1621 caatattggc cgcgacgccg gaggcccgcg cggcttcgag gtcggcacgg tgttcgacga
     1681 tatcatcgac ggtcgcgacc gcgaggacga gctgcacggc ggcatcggca acgacgtgct
     1741 gatcggcggc gtcaacgatg atgcgctgtt cggcggcgcc ggccaggaca cgctggtcgg
     1801 cggcacgggc accgacaacc tggcgggcgg agagggcaac gacttgctga tgggcgggca
     1861 cggccgcgat tacatttcgg gcggtgcggg caccgacacc atctcctacc gtggcgaggc
     1921 gcgcgcggtc accatcaacc tggccgccgg caccgtgcac agcgatgccg cgcacaatgc
     1981 ggtggtgctg ccggatccgg ccacgaccat caacaacctg ccggcggacg cgctgaccga
     2041 tgccaacctc gaggatctga tcggacagat cgtcgaggtc gaccacgagg cggagatctt
     2101 cgagttccag tcggccgccg atatcgagaa tgccgagggc ggcctcggca acgacaccat
     2161 catcggcaac gacggcgaca acgtgctgac cggcggcaag ggcaacgaca gcctggacgg
     2221 cggagccggc accgacacgg cactattctc gggtgctgcc gacgagtaca gggtcacaag
     2281 caacgcggat ggctccttca cggtcgaaga tctgctggcc aaccgcgacg gcaaggacat
     2341 tatccgcaat atcgagctgc tgcagttcgc tgacctggcc gcggcgccgg acgctgtggc
     2401 ggaggaacca cctgccccac cgatcgccgt cggcaacgaa gtcacagcgt ccgagtccga
     2461 tgccgaaggc ggcgtgatca tcaatgtgct ggccaacgac acaggcgcgg atctgcgtgt
     2521 ggcgcagatc aatggcgtta cgatcactac gcagtcgccg gtgcagatcg cgagcggctt
     2581 cgtgcaattg cgcgaggacg gccagctgct gttcaccgcc aacctcaact tcagcggggt
     2641 ggcggcattc gcctacacca tcgccaatac ggcgggcctt cttgcgacgg ccaacgtcac
     2701 cgtcaacgta acgcccgtaa acgacgcgcc caccgcggtt gcgctctcca atcttcatgt
     2761 ggacgagcac gtcgccggtg cagtgatcgg taccctgtcg gcagtcgatc cggaccagcc
     2821 cgacacgcac accttcaccg tgtcggatca gcgcttcgag gtggtcggca cgaccctcaa
     2881 gctcaagtcc ggcgtaagcc tcgacttcgc gacggcacac accgtcgacg tgaccgtcac
     2941 agccacggat tctggcctcc ttaccacgtc gcagagcttc accatcgacg tgatcggcgg
     3001 caacgcggcg ccgacctcga tcacgccgga cgcggccatg gtggcggaga acgcgctatt
     3061 gggcacgacg gcggtttccg gcctcgcggc tgtcgatccc aacgtcggcg atcgtgcagg
     3121 cttctatctc gtcaacgacg tgggcggccg cttcgtaatg attggcgaca cgattattgt
     3181 cgccaatccg acgctgctcg actacgaagc cggcagcacg cacaccattg aggtcaaggc
     3241 agtcgacacg ggcggcctat acaagaccca atccgtcacg gttacgctcg gcaacctcgt
     3301 gggtggcgcc gacaacccca tcaacggaac ggccggcaag aacgcgatca acgggacttc
     3361 gggtgccgac cgtatcaacg gcctcggcgg cgaggaccgc atgcagggtg gcgcaggcga
     3421 cgacatcctg gatggcggcg ccggcaaaga ccgcctggcc ggaggcgccg gcaacgacac
     3481 ctacgttgtc gatcagacgg acgatcgggt cgaagaggac aggaaccagg gcaccgacac
     3541 ggtcgtgacc tccctcgaca gcttcgggct cggcgacgac gtcgagaacc tcgtctacac
     3601 cggcaccagg tcgttcaccg gcacgggcaa cgacgacgcc aacaccatcc tgggcggctc
     3661 gggtgccgac aacctgcgcg gcggtcgggg cgacgacacc ctggtcggcc gcggcggcaa
     3721 cgacacgctg gacggcggtg acggccatga cgcgctgcac ggcggtgacg gcaacgacac
     3781 tctgacgggc ggtgctggca acgacatcct cgagggtggg gccggcagcg acatcctgaa
     3841 cggcggcgtc gacaacgata cgttcgtgtt ccggcccggc ttcggcaacg acaccatcaa
     3901 agagttcggc gacagcacca acaatcagga tgtgctggac ctgtcgatgt cgggctatgc
     3961 gacgtttgcg gccctgcagg cggccggcgc cttggcccag gtcaatgccg acgtggtggt
     4021 cacgctcaat ccactcgatc cgatcacgtc cgacaagatc accttgaaga ccgtcaacct
     4081 gtcgacgttg gatgccacgg acttcaagtt tggctagcgt gtcacgccaa ccttgtacgt
     4141 ggctaataac gttaaggaat cactgagaat aaggatgtgt ttcccggcgc ttgggtttag
     4201 gttcgtgggc cggcacggat cataggtggg gcgctttttc ggcgcgatcc ggactttggg
     4261 ggagtattgc gtagtggctg ggcggccgga aaagaagaaa agcgaggtaa ggctcgccgc
     4321 aggggcgttg ggctccgttg ttattaccct ggccgtcgtc tcgggtgtcg ttaacattct
     4381 ggcgctgacc ggctccttct acatgctgca ggtctacgac cgggtgctgt cgagccgcag
     4441 catggcgacc ctgcttgccc tctccttact ggccgtcggc ctctatttgt ttcagggcgg
     4501 cctggaagtg atccgctcgc agatcctggt gcgccttgcc ggtcggctcg accggcaact
     4561 gaccaagcag gccatgacgg ctgccatgcg cttgcgcgcc tacgtcggcg cacagggcag
     4621 cccgctgcaa ccgatccgcg atgtcgatgc ggtgcgcacc tacctgtccg ggcaggggcc
     4681 ggtggcgatc ctcgacatgc cgtggatgcc gctctacctc gccttcgtgt tcatcctgca
     4741 tccgatgctc ggctggatca cgatgttcgg cgccgccttc ctgctgtcgg tggcgttcct
     4801 gaccgagcgc atgatgcaga cgcctggcga ggtgctggca tccgcctcca agcagcgctg
     4861 ggccattgcc gaggcctgcg agcagaactc cgatgccctg cacgccatgg gctttggcca
     4921 tcgcttcttg cgccggttcc aggcggtcaa caaccagcac ctggacgcca acgagcggtt
     4981 gagcgacgtg gtgggcggcc tgtcgtcggt atcgcgcgtg ttccgcatga tgctgcagtc
     5041 ggcgctgctt ggtatcggcg cctatctgac catcaagggc gagatgtcgg caggcgccat
     5101 catcgcctgc tccatcgccg cgtcgcgggc gctggcgccg atcgaggtgg cgatcggcaa
     5161 ctggcgggtg ttcgtggccg cgcgacaggc ctgcgcccgg ctcgatgcgg tgctggccaa
     5221 cctgcccgag aaatcgcgcc ccctgatgct gccggccccc acgcacgcgc tcagcgtcga
     5281 gagcatttcg gtcatggtgc cgggctc
//
LOCUS       DASRJA010000183         8381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2162336,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000183 DASRJA010000000
VERSION     DASRJA010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162336"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VFC01_08290"
     CDS             complement(<1..928)
                     /locus_tag="VFC01_08290"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216348.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HZN79671.1"
                     /translation="MFDHNQPNFADRHIGPDADAVATMLKTIGVGSLDELADKALPAG
                     ILDALSDDGVAPGLDQLLPAASEQESLAELRAMADSNTVAVSMIGQGYFDTLTPPVLR
                     RNILENPAWYTAYTPYQPEISQGRLEALLNFQTMVADLTGLEVANASMLDEGTAAAEA
                     MTLMQRAVKGPSNRLAVDTDLYRQTAAVLATRAEPLGIEIVTADLRGGLPDGDFFGVI
                     TQLPGASGAVVDWTDLVAAAHERGALVAVGADLLALTLIASPGDIGADVAFGSTQRFG
                     VPMGFGGPHAGFLAVHAKHARQLPGRLVGVSQD"
     regulatory      complement(1012..1110)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1153..1776)
                     /locus_tag="VFC01_08295"
     CDS             complement(1153..1776)
                     /locus_tag="VFC01_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZN79672.1"
                     /translation="MGDTPRQEQLDLSTSSGPAESEASTPSEPVQAGLFPDDSVPDEL
                     VGYRGPSACQIAGITYRQLDYWARTSLVVPSIRSAAGSGSQRLYSFKDILVLKIVKRL
                     LDTGISLHNIRVAVDHLRQRGVRDLANITLFSDGTTVYECTSAEEVVDLLQGGQGVFG
                     IAVSGAMRELTGAIAEFPGERADGGESIATPEDELASRRKHRDRKIG"
     gene            complement(1978..2472)
                     /locus_tag="VFC01_08300"
     CDS             complement(1978..2472)
                     /locus_tag="VFC01_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HZN79673.1"
                     /translation="MGEVRVVGIRVEQPQNQPVLLLREANGDRYLPIWIGQPEAAAIA
                     LEQQGHEPARPLTHDLIKDVIAALGHSLKEVRIVDLQEGTFYADLIFDRDIKVSARPS
                     DSVAIALRVGVPIYVEEAVLAEAGLLIPDENDEEAAGAVREDEVEKFKEFLDSVSPDD
                     FKAT"
     gene            complement(2520..3254)
                     /locus_tag="VFC01_08305"
     CDS             complement(2520..3254)
                     /locus_tag="VFC01_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZN79674.1"
                     /translation="MTAPDRPALAGMSIGAVLDLLRGDFPDVTISKIRFLEAEGLVTP
                     ERTPSGYRRFTAYDCARLRFILTAQRDQYLPLKVIKAQLDAQPDGELPASGSAYGVPR
                     LVPVSSEDTEAGISGAVQSQVRLRREDLLARSGIDEALLGELVKGAVIRPGPAGFFDE
                     HSVVIAQCAKALAEYGVEPRHLRAFRSAADRQSDLIAQIAGPVVKAGKAGARDRADDL
                     AREVAALAITLHTSLIKSAVRDVLDR"
     gene            complement(3258..3737)
                     /gene="garA"
                     /locus_tag="VFC01_08310"
     CDS             complement(3258..3737)
                     /gene="garA"
                     /locus_tag="VFC01_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen accumulation regulator GarA"
                     /protein_id="HZN79675.1"
                     /translation="MTEKDHNSGADQSEDVTVETTSVFRADFLNELDAPATAGTEGAV
                     SGVEGLPAGSALLVVKRGPNAGSRFLLDQPTTSAGRHPDSDIFLDDVTVSRRHAEFRL
                     EGGEFQVVDVGSLNGTYVNREPVDSAVLANGDEVQIGKFRLVFLTGPKGDDDSGPGS"
     gene            complement(3890..4285)
                     /gene="gcvH"
                     /locus_tag="VFC01_08315"
     CDS             complement(3890..4285)
                     /gene="gcvH"
                     /locus_tag="VFC01_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZN79676.1"
                     /translation="MSEIPADLSYTEEHEWVQRTGDDTVRVGITDYAQSALGDVVFVQ
                     LPDVGADVTAGESFGEVESTKSVSDLYAPVSAKVVAVNGDLEANPQLVNSDPYGEGWL
                     VDLQIDAAALDEGLRGLLDADGYRATVTE"
     gene            complement(4318..5139)
                     /locus_tag="VFC01_08320"
     CDS             complement(4318..5139)
                     /locus_tag="VFC01_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HZN79677.1"
                     /translation="MSDTHGQHEAEPDQHGRHELPADEPAPQIGDLHAGGVKGLVRRG
                     RSQIVFGALAVLLLVLLGVAIVTQARQTESGDSLETARPADLLVLLDSLQQREAALNT
                     EVADLQRTLAQLQESGSSDAAAIENAQARLAALSILIGTVAATGPGVTLTIEDKAPGV
                     PAETVLDVINELRAAGAEAMEIRGGSAAVRVGVDTWVVGTPGALVVDGATLNPPYSVL
                     AIGDPPTLAAAMNIPGGAMDSVERVGGTMVVQQSDRVDVTALRQPKPRQYAQPVK"
     gene            complement(5144..5476)
                     /locus_tag="VFC01_08325"
     CDS             complement(5144..5476)
                     /locus_tag="VFC01_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HZN79678.1"
                     /translation="MIGIAALVIGIVLGLVFHPSVPEVIQPYLPIAVVAALDAVFGGL
                     RAYLERIFDSKVFVISFVFNVLVAALIVYVGDQLGVGTQLSTAIIVVLGIRIFGNAAA
                     LRRRLFGA"
     gene            complement(5490..6392)
                     /locus_tag="VFC01_08330"
     CDS             complement(5490..6392)
                     /locus_tag="VFC01_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HZN79679.1"
                     /translation="MNTLGGYDPQAGRNAHEANTPTLIPVPSLLRSLLSDHLDPGYAA
                     AAEAKARGEKRRKWWQASTWQICGALLIAAVFAAAVAQARSTAPGVRETQQVLAGSVR
                     SAEATANGVTARRNALASEVDAERRSRLEGDERGQQLLGNLDQANFAAAATPVIGPGL
                     TVTVTDPGMSKDLSDVSKERVPGSQQVILDRDLQLVVNSLWVSGAEAISVGGVRIGPN
                     VTIRQAGGGILVDNQPISSPYAILAVGPPHAMQDVFDRSPGLQRLRLLETSYGVGVNV
                     SSGDGLTLPAGSVRDINFAKQIGP"
     gene            complement(6389..6985)
                     /locus_tag="VFC01_08335"
     CDS             complement(6389..6985)
                     /locus_tag="VFC01_08335"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZN79680.1"
                     /translation="MAGAAGGVRDRVLTIPNALSVLRLVLVPVFLYLLLVVHATGWAV
                     GVLMFSGFSDWADGKIARLVDNQSSRLGELLDPAVDRIYMVTVPVALAIYGAVPWWIV
                     LTLLGRDVVLAATLPVLRSRGLTALPVTYIGKAATFALMSGFPLILLGQFDTLWSRII
                     LACGWAFLIWGMAMYLWSGVLYLIQVAMVVRQLPKVST"
     gene            complement(6995..>8381)
                     /gene="secA2"
                     /locus_tag="VFC01_08340"
     CDS             complement(6995..>8381)
                     /gene="secA2"
                     /locus_tag="VFC01_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HZN79681.1"
                     /translation="HYIVRDDAVHLINASRGRIAQLQRWPDGLQAAVEAKEGIETTET
                     GEVLDTITVQALINRYPTVCGMTGTALAAGEQLRQFYKLGVSPIPPNTPNIREDDTDR
                     VYITAAAKNDAIVEHIKEIHETRQPVLVGTRDVAESEELHERLVKAGIPAVVLNAKND
                     AEEAAVIAEAGTLGTVTVSTQMAGRGTDIRLGGSDEADHDEVAELGGLHVIGTGRHHT
                     ERLDNQLRGRAGRQGDPGSSVFFASWEDDVVLAHLESNKLPMESDDDGRIVSKKAATL
                     LDHAQRVAEGRMLDIHANNWRYNQLIAQQRAIIVERRDTLLRTATAREELAERSPKRY
                     EEFVEDLGEEKLEKICRLIMLYHLDRGWADHLAYLADIRESIHLRALGNQTPIDEFHR
                     MAVDAFASLAADAIEAAQQTFETAASIEDEPGVDLSKLARPTSTWTYMVHDNPLADDT
                     MSALSLPGVFR"
BASE COUNT         1357 a         2829 c         2766 g         1429 t
ORIGIN      
        1 cgtcctgtga gacgccgacc agccggccag gcagctgacg ggcgtgcttg gcgtgcactg
       61 ccagaaaacc ggcgtgtggt ccgccgaatc ccattggcac accgaatcgt tgggtgctgc
      121 caaacgcgac gtcggcaccg atgtcgccgg gtgatgcgat cagtgtcagg gccagcaggt
      181 cggcgccgac cgccaccagc gcaccgcgct cgtgcgcggc cgccaccaag tcggtccagt
      241 cgaccaccgc gccgctggcg ccgggcagct gggtgatcac cccgaagaag tcgccgtcgg
      301 gcaggccgcc acgaaggtcg gcggtgacga tctcgatgcc cagcggctcg gcacgggtgg
      361 ccagcacagc cgcggtctgg cggtacaggt cggtgtcgac ggccagccgg tttgacgggc
      421 ccttgacagc ccgttgcatc agcgtcatcg cctcggcggc ggcggtgccc tcgtccagca
      481 tcgaggcgtt ggccacctcg agcccggtca ggtcggcgac catggtctgg aagttcagca
      541 gcgcctccag gcggccctgg ctgatctcgg gttgataggg ggtataggcg gtgtaccacg
      601 cggggttctc aagtatgttg cggcgcaaca ccggtggcgt cagcgtgtcg aagtagccct
      661 gcccgatcat cgatacggcg acggtgttcg agtcggccat cgcgcgaagt tcggccagcg
      721 attcctgctc gctggcggct ggcagcagct gatccaggcc gggagcgacg ccgtcatcag
      781 acagcgcgtc gagaatgcct gcgggcagcg ccttgtcggc cagttcgtcc agcgacccca
      841 cgccgatggt cttgagcatc gtcgcgacgg catccgcgtc cggtccgatg tggcgatcgg
      901 cgaagttggg ctgattgtga tcgaacactg acgtgcactc cgttagggct cagaaccgct
      961 gggttcctcc ccctcagtcg tcaacccgcc cgggcgtctc cagaggcatc ggggccttgc
     1021 gcggtcctgg gtgcctgaga ggttgacgga gaggtgttgc tccttcggcg tccgtggctg
     1081 gcggtcacgg aactctcccg cacaagggcg atgcgctaac gagtttaccg gcaggccggc
     1141 gaacctgtgg tatcagccga tcttgcggtc gcggtgttta cgccgcgagg ccagctcgtc
     1201 ctcgggcgtg gcgatcgact cgccgccgtc ggcgcgctca ccggggaatt cggcgatcgc
     1261 cccggtcagc tcccgcatgg cgccggacac cgcgatgccg aacacgccct ggccgccctg
     1321 cagcaggtcc accacttcct cagccgacgt gcactcgtaa accgtggtgc catccgagaa
     1381 cagcgtgatg ttggccagat cgcgcacccc gcgctgccgc agatgatcga cggcgacgcg
     1441 aatgttgtgc agcgagatgc cggtatccag cagccgcttg acgatcttga gaaccaggat
     1501 gtctttgaac gagtacagcc gctggctacc cgacccggcc gcgctgcgga tggacggcac
     1561 gacgagcgag gtccgcgccc agtaatccag ctggcggtag gtgattccgg cgatctggca
     1621 ggcgctcggc ccgcggtagc cgacgagctc gtcaggcacc gagtcatcgg ggaacagacc
     1681 cgcctgcacg ggttcgctgg gcgtactggc ttcagattca gcgggcccgc tgctggtgga
     1741 caggtccagc tgctcttgac gtggcgtgtc tcccactctc gaatcctctc gccgatcgaa
     1801 ctcgcgtctg tgacctgcat gggcgcacgt ttgccgaagc tcgaatgtgt cgagcatacg
     1861 cttcccgtcg agccgttact gctcgtcgac gtcaagtatg gctgcccaat tcgcggttgc
     1921 ggaaaacccg cgccgcgtgt cgagtgcgac gagacgtttc cgtgacaaaa ctccctgcta
     1981 ggtcgccttg aagtcgtcgg gcgacacgct gtcgaggaac tccttgaact tctcgacctc
     2041 gtcctcacgg acagcgcctg ccgcttcctc gtcgttctca tcgggaatca gcaggccggc
     2101 ctcggcgagc accgcctcct ccacgtagat cgggacgccg acgcgcagcg cgatggcgac
     2161 cgaatccgac ggccgtgccg acaccttgat gtcgcggtcg aagatcaggt ccgcgtagaa
     2221 agtgccctcc tggagatcga cgatgcgcac ctctttaagc gaatgaccaa gcgcggcaat
     2281 gacatctttg atgaggtcgt gcgtcaacgg ccgcgcgggc tcgtggccct gctgttcgag
     2341 cgcgatcgcc gcggcttccg gctggccgat ccaaatcggt aagtagcggt caccgttggc
     2401 ctcccgcagc agcagcactg gctggttctg cggctgctcc acgcgaatgc cgaccacacg
     2461 aacttcaccc atctgtgtct gccctccgca cgaaagaagc cttgaaatga gtctaatcct
     2521 cagcgatcca gaacgtcgcg tacggcggac ttgatcagcg acgtgtgcaa ggtgattgcc
     2581 agcgcggcca cctcgcgggc cagatcgtcg gcacggtcac gggcaccggc cttgcccgcc
     2641 ttgaccaccg gacccgcgat ctgggcgatc agatcggact ggcggtcggc agccgagcgg
     2701 aatgcgcgca gatggcgtgg ttccacgccg tactccgcca gggccttcgc gcactgggcg
     2761 atcaccaccg agtgctcgtc gaagaagccg gccggaccgg gcctgatgac ggcgcccttg
     2821 accagctcgc ccagcagagc ctcgtcgatc cccgaccgag cgagcagatc ctccctgcga
     2881 agccgcacct gcgactgcac cgcccccgat atcccagcct cggtgtcctc gctcgagaca
     2941 ggaaccaaac gcggtacgcc gtaagcggat ccgctcgcag gcagctcgcc gtcgggctgg
     3001 gcgtcgagct gtgccttgat gaccttcaac ggcagatact ggtcgcgctg ggctgtcagg
     3061 atgaaccgca ggcgcgcgca atcgtaggcg gtgaaccgcc gatatcccga cggcgtccgc
     3121 tcaggggtga ccaggccttc ggcttccaga aaccggattt tggagatggt cacgtccggg
     3181 aagtcgcccc gcagcagatc gagaaccgct ccgatcgaca tcccggccaa agcgggtctg
     3241 tcgggggcgg tcatcgctta gctgcccggg ccgctgtcgt cgtcaccctt gggcccggtc
     3301 aaaaacacca ggcggaactt gccgatctgc acctcgtcac cattcgccag caccgcggaa
     3361 tcgacgggtt cccggttgac gtaggtgccg ttgaggctgc cgacgtcgac cacctggaac
     3421 tcgcctccct ccagccggaa ctccgcgtgc cggcggctga ccgtgacgtc gtcgaggaag
     3481 atgtcgctgt cgggatgccg accggccgac gtggtgggtt ggtcgagcag gaatcgcgag
     3541 cctgcgttgg gtccgcgctt gacgaccagt agggccgacc cggcgggcag cccttcaacg
     3601 cccgagaccg caccctcggt gccggccgtg gccggagcgt ccagctcgtt gaggaagtcg
     3661 gcgcggaaga ccgatgtcgt ctccacggtg acatcctcag actggtcagc cccagagttg
     3721 tggtcctttt ccgtcacccg ctgctcctca ctggctgctg tggcgatatc aggccgggcc
     3781 ccccagccat gatgtctggt gtcgaccgta ccgcgcagtg gacatcattg tgtccaccac
     3841 cgccggatcg gcctgtgttg gatttcggtc tgaaaaccct aacaactcgt cattcggtca
     3901 cggtcgcgcg gtagccgtcc gcatcaagta acccgcgcag tccctcgtcg agggcggcgg
     3961 cgtcgatttg cagatcgacg agccagcctt cgccgtaggg gtcggagttg accagttgcg
     4021 ggtttgcttc cagatcaccg ttgaccgcaa cgactttcgc gctgacgggc gcgtaaaggt
     4081 cggacacgga cttggtcgac tccacctcac cgaacgactc gcccgccgtg acgtcggcgc
     4141 cgacgtcggg caactggacg aacaccacgt cgccgagcgc cgactgggca tagtcggtga
     4201 tgccgacccg tacggtgtcg tcgccggtgc gctgtaccca ctcgtgttcc tcggtgtagg
     4261 acaggtcggc tgggatttcg ctcacggcgc tcctcgtgga caggctcaga cgaatgctca
     4321 cttgacgggc tgagcgtatt ggcggggttt cggttgccgc aaggcggtta cgtcgacgcg
     4381 gtcggactgt tgtaccacca tcgtgccgcc aacgcgctcg acgctgtcca tggcgccgcc
     4441 gggaatgttc atcgccgcgg ccagcgtcgg cggatcacca atcgccagaa ccgaatacgg
     4501 cgggttcagc gtcgcgccat ccacgacgag cgcgcccggg gtcccgacta cccaggtgtc
     4561 gacgccgacc cggacagccg ctgacccgcc gcggatctcc atggcctcgg caccggccgc
     4621 ccgcaactcg ttgatcacgt ccagcacggt ctcggcgggc acccccggcg ccttgtcctc
     4681 gatcgtcagc gtcacaccgg gaccggtcgc ggcgacggtg ccgatcaaga tcgacagcgc
     4741 ggccagccgg gcctgcgcgt tctcgatcgc ggccgcatca ctgctgcccg attcctgcag
     4801 ctgcgccagc gtgcgctgca ggtcggccac ttcggtattc agcgcggcct cccgctgctg
     4861 cagcgaatcg agcaacacca gcagatcggc gggccgcgcc gtctccagtg agtcgccgga
     4921 ttcggtctga cgagcctggg tgacgatggc caccccgagc aggaccaaca gcagcacggc
     4981 gagagcgccg aacacgatct gcgacctgcc ccgccgcacc agtcccttga caccgccggc
     5041 atgcagatcg ccgatctgcg gtgccggttc gtcggcgggc agttcgtgcc gaccgtgttg
     5101 atccggctcg gcttcgtgct gcccatgggt gtcgctcatg gatctatgcc ccgaaaagcc
     5161 tgcgtcgcaa cgcggctgcg ttgccgaaga tccgaatgcc cagcacgacg atgatggcgg
     5221 tggacagctg ggtgccgacc cctagttggt cgccgacgta gacgatcagc gcggcaacga
     5281 gcacgttgaa gacgaatgag atcacgaaga ctttcgagtc gaagatgcgc tccagatagg
     5341 cccgcaatcc gccgaacacc gcgtcgagcg cggcgaccac cgcgatcggc aggtacggct
     5401 ggatgacttc gggaacgctc gggtgaaaca ccagaccgag cacgatcccg atgacaagtg
     5461 cggcgattcc gatcatttgt gtcccgtcac tagggcccaa tctgcttggc gaagttgata
     5521 tctcgaaccg aaccggccgg cagcgtgagg ccgtcgccgg agctgacatt gaccccgacg
     5581 ccgtagctgg tctccagcaa ccgaagtcgc tgcaggccgg gactgcggtc gaacacgtcc
     5641 tgcatggcat gcggcggtcc gaccgcgaga atcgcgtagg ggctgctgat cggttgattg
     5701 tcgacgagga tgccgccgcc cgcctgccgg atggtgacgt tcggaccgat tcgcactccg
     5761 cccaccgaga tggcctcggc gccgctgacc cacagcgagt tgacgaccag ctgcagatcg
     5821 cgatccagga tcacttgctg gctgccgggg acgcgctcct tagagacgtc gctgaggtct
     5881 ttggacatcc ccggatcggt gacggtcacg gtcaggcccg gcccgatcac gggcgtggcg
     5941 gccgccgcga agttcgcttg atcgagattg cccagcagct gctgaccgcg ttcgtcgccc
     6001 tcgagccggc tgcgccgttc cgcgtccacc tccgaagcca gcgcgttgcg gcgggcggtg
     6061 acgccgttgg cggtggcttc cgccgacctg acgctgcccg ccagcacctg ctgcgtttcc
     6121 cgcacgccgg gtgcggtcga gcgggcctgg gccaccgccg cggcgaacac cgccgcgatc
     6181 agcaaagcgc cgcagatctg ccacgtcgac gcctgccacc acttccgccg cttttcgccg
     6241 cgcgctttcg cctccgccgc cgcggcgtag cccgggtcga ggtggtcgga cagcagcgag
     6301 cgcagcagcg atggcaccgg gatcagcgtc ggcgtgttgg cctcgtgagc gttgcggccg
     6361 gcttgggggt cgtagccgcc cagcgtgttc atgtcgacac cttcggcagt tggcgcacca
     6421 ccatcgccac ctgaatcagg tacagcaccc ccgaccacag gtacatggcc attccccaga
     6481 tcaagaacgc ccagccgcag gccaggatga tgcggctcca cagcgtgtcg aactggccga
     6541 gcaggatcag cgggaagccc gacatcaggg cgaaggtggc agccttgccg atgtaggtga
     6601 ccggcagcgc ggtcaatccc cggctgcgca gcaccggcag ggtcgcagcc agcacgacgt
     6661 cacgcccgag cagcgtgagc acgatccacc agggcaccgc gccgtaaatc gcaagcgcca
     6721 caggcactgt caccatgtag atgcggtcca cagccgggtc gagcagctcg cccagccgcg
     6781 acgactggtt gtcgaccagc cgcgcgatct tgccgtcggc ccagtcggag aatccgctga
     6841 acatcagcac gccgaccgcc cagcccgtgg cgtgcacgac gagcagcagg tagaggaaga
     6901 ccggtaccag gaccaggcgc agcaccgaca gcgcgttggg tatcgtcagc acccggtcgc
     6961 gcacaccgcc cgctgcgccc gccatgggct aaacctatcg gaagacgccg ggcaggctca
     7021 gcgccgacat ggtgtcgtct gccagcggat tgtcgtgcac catgtacgtc cacgtcgacg
     7081 tcggccgggc cagtttggaa aggtcgacgc ccggctcgtc ctcgatcgag gcggccgtct
     7141 cgaaggtctg ctgggcggcc tcgatcgcgt cggcggccag cgaggcgaag gcgtccaccg
     7201 ccatccggtg gaactcgtcg atcggggtct ggttgcccag ggcccgcaga tgaatgctct
     7261 cccggatgtc ggccaggtat gccagatggt cggcccagcc gcggtccagg tggtagagca
     7321 tgatcagccg gcagatcttc tccagcttct cctcgccgag atcctcgacg aattcctcat
     7381 agcgcttcgg tgagcgttcg gcgagctcct cgcgcgcagt cgcggtgcgc agcaaggtgt
     7441 ctcgacgttc gacgataatc gcgcgctgtt gggcgatcag ctggttgtag cgccagttgt
     7501 tggcgtggat gtcgagcatc cggccttcag cgacgcgctg ggcgtggtca agcagggtgg
     7561 ctgccttctt gctgacgatg cggccgtcgt cgtcggattc catcggcagc ttgttggact
     7621 cgaggtgggc cagcaccacg tcgtcttccc agctggcgaa gaacaccgag gagccgggat
     7681 cgccctggcg tccggcgcga ccgcgcagct gattgtccag ccgttcggtg tggtggcgac
     7741 cggtgccgat gacgtgcagt ccgccgagct cggcgacctc gtcgtgatcg gcctcatcgg
     7801 aaccgccgag gcggatgtcg gtgccgcggc ccgccatctg cgtggacacg gtgaccgtac
     7861 cgagcgtgcc tgcctcggcg atgacggcgg cctcttcggc gtcgttcttc gcgttcagca
     7921 ccaccgccgg gatgccggcc ttgaccagcc gctcgtgcag ctcctcggac tcggcgacgt
     7981 cgcgggtgcc gaccagcacg ggctgacgag tctcgtgaat ctccttgatg tgctcgacga
     8041 tcgcgtcgtt cttggcggcc gcggtgatgt acacccggtc ggtgtcgtcc tcacgaatgt
     8101 tgggagtatt cggcggaatt ggcgacacgc cgagcttgta gaactgccgc agctgttcgc
     8161 cggcggccag cgcggtgccc gtcatgccac acaccgtggg ataccgattg atcaaggcct
     8221 gcaccgtgat ggtgtcgagg acctcgccgg tttcggtggt ctcgatgccc tccttcgcct
     8281 cgaccgcggc ctgcagcccg tcgggccagc gctgcagctg cgcgatgcgg ccgcgggagg
     8341 cgttgatcag gtgcaccgcg tcgtcacgca cgatgtagtg c
//
LOCUS       DASRJA010000184         4595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3536727,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000184 DASRJA010000000
VERSION     DASRJA010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3536727"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..741
                     /locus_tag="VFC01_08345"
     CDS             142..741
                     /locus_tag="VFC01_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3153 domain-containing protein"
                     /protein_id="HZN79682.1"
                     /translation="MAVGCVRVRASITVSPDDRVSGQIVAAAKPRNADDKGPQLLNSL
                     PFAQKVAVSDYNRDDYVGSQAVFSDLTFAELPQLANMNRDAAGVDISLRRAGDLVILE
                     GRADLTSLNDPEADVSLSVAFPGEVTSTNGDQVSSEVVEWKLRPGVVSTMNAQARYTD
                     PSARSFTGAAIWLAIASFLVAGIVGGVAYYSRDRSPRPT"
     gene            869..1957
                     /gene="idsA2"
                     /locus_tag="VFC01_08350"
     CDS             869..1957
                     /gene="idsA2"
                     /locus_tag="VFC01_08350"
                     /EC_number="2.5.1.1"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004161 - dimethylallyltranstransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0033384 - geranyl diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (2E,6E)-farnesyl/geranyl
                     diphosphate synthase"
                     /protein_id="HZN79683.1"
                     /translation="MDAAAPSAVELASAVTGQLREYLRNRRSDAAYIGTDYAELTAAL
                     EEFVLRGGKRLRPAFAYWGWRAVADADQDPVDPSMLRLFSALELLHACALVHDDVIDA
                     SATRRGLPTVHRLFTDKHRTCQWHGSSEQFGLSAAILLGDLALVWADDIVATADLPAD
                     ARLRVRRVWSAIRTEVLGGQYLDIVAESSGAESVASAMTVNIYKTASYTISRPLQFGA
                     SAAADRPDVLAAFHELGTSLGVAFQLRDDVLGVFGDPAVTGKPSGDDLRSGKRTVLLA
                     EAVERAEKVDPVAAKLLRTSIGTELTDAAVKELCLVIESVGALAAVEERIDALTRRSL
                     EILNAASIDVQAKVGLSELARLAANRSA"
     gene            1962..3527
                     /locus_tag="VFC01_08355"
     CDS             1962..3527
                     /locus_tag="VFC01_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->6)-mannopyranosyltransferase A"
                     /protein_id="HZN79684.1"
                     /translation="MTTPTPTQVPKTGPAHHLWRLTSFATSPQARPALLGALGAVMIT
                     AGGLGAGSTRLHDPLLESLHVSWLRFGHGLVLSSVLLWIGVALMLIAWLWLGRRVIDR
                     TANEYTMVATTGFWLAPLLLSVPVFSRDTYSYLAQGALLRDGLDPYVVGPIDNPNSLL
                     DNVSPIWTTTTAPYGPAFILIAKFVTMLVGNDVVAGTMLLRLCMLPGLALLIWAAPRM
                     ARHVGADGAAALWICALNPLVIIHLMGGVHNEMLMVGLMVAGIALTFGGHHIWGVGLI
                     AVAVAVKATAGLALPFMVWVWMRHLRDGRGYSPTKAFAAATAASAVIFVAVFAVLSWL
                     AGVGLGWLTALAGSVKIINWLTVPTAAANLVNVFGSLLLPVNFYAVLDITRIIGIAVI
                     AISLPLVWWRFRHTDREALIGIAVVMIVVVLFVPAALPWYYTWPLAVVAALAQTRQAI
                     AVIAGLSTWITVIWKPDGAHGMYSWIHVLLATACAAVAWYSLYSAPNTPSPGPDAPLP
                     GPDASAARPPAGH"
     gene            complement(3448..3852)
                     /locus_tag="VFC01_08360"
     CDS             complement(3448..3852)
                     /locus_tag="VFC01_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="HZN79685.1"
                     /translation="MSSIPAADDVLDPGEAVYDLPSVADLLGVPVTKVQQQLREGHLV
                     AVRREGAVVVPKAFFDDTGHVVKSLAGLLVVLHDGGYRDTEIVRWLFTPDPSLTIRRD
                     GTTDRQANARPVDALHSHQAREVVRRAQAMAY"
     gene            3889..>4595
                     /locus_tag="VFC01_08365"
     CDS             3889..>4595
                     /locus_tag="VFC01_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN79686.1"
                     /translation="MSSVEAYQQSDPLVGAVLDGRYRVEAMIATGGMSAVYRGLDLRL
                     DRPVALKIMDSRYAGDNQFLTRFQREARVVARLKDPGLVAVYDQGVDGQHPFLVMELI
                     EGGTLRELLRERGPMPPHAVAAVLRPVLSGLAVAHQAGLVHRDIKPENVLISDDGDVK
                     IVDFGLVRAVAEAKITSTSVILGTAAYLSPEQVSTGDAGPRSDVYSVGILAYELLTGT
                     TPFTGDSALAVAYQRMD"
BASE COUNT          690 a         1486 c         1574 g          845 t
ORIGIN      
        1 cacttcgccg gcgatccgcg cgccttcgcg attctgggcc gggctctgcc cctgtaaggg
       61 cgctggatct ggcacgatga cgacgtgccc gcccggttcc gcaagctcgc cgtcgcgttg
      121 ctgctgctgc tcatcgtgcc gatggcggtg ggctgtgtgc gggtgcgggc atcgatcacg
      181 gtgtcgccgg acgaccgggt gtcgggtcag atcgtcgcag ccgccaagcc ccgcaacgcc
      241 gacgacaaag gcccgcagtt gctcaacagc ctgccgttcg cccagaaggt cgcggtgtcg
      301 gactacaacc gcgacgatta cgtcggctcg caggccgtgt tctccgacct gaccttcgcc
      361 gagctgcccc aactggcgaa catgaaccgg gacgccgcgg gcgtcgacat ctcgctgcgc
      421 cgcgccggcg acctggtcat cctcgaaggt cgcgccgacc tcacgtcgct caacgacccg
      481 gaggccgacg tttcgctttc ggtggcgttc ccaggcgagg tgacctcgac caacggtgac
      541 caggtgtcgt cggaggtcgt cgaatggaag ctgcgccccg gcgtggtcag cacgatgaac
      601 gcccaggccc gctacaccga tcccagcgca cgttcgttca ccggagcggc gatctggctt
      661 gccatcgcgt cgtttctggt ggccgggatc gtcggcgggg tggcgtacta cagccgggac
      721 cgatcgcccc ggccgacctg attgtcagcg aatgcccctg gtggatgtgc gccctgactt
      781 ggtacaaaac cagtcgcacg ctctactctg aatgcactcg gggggcgagt actagagact
      841 tacgttcgga aaggggcgcc cgctgagcgt ggatgcagcg gcaccgtcag ccgtcgagtt
      901 ggccagcgcc gtcaccggcc aattgcggga gtatctccgc aatcgtcgca gtgacgccgc
      961 ttacatcggc accgactacg ccgagttgac cgcggccctt gaggaattcg tactccgcgg
     1021 cggaaaacgc ctgcgcccgg ccttcgccta ttggggatgg cgcgcagtgg cggatgcaga
     1081 ccaggatccc gtcgatccgt cgatgctgcg gctgttctcg gccctggagt tgctgcatgc
     1141 ctgcgcactg gtgcacgacg atgtgatcga cgcgtcggcc acccgccgcg ggctgccgac
     1201 cgtgcatcgg ctgttcaccg acaagcacag gacctgccaa tggcacgggt cgtccgaaca
     1261 gttcgggctc tcggccgcga ttctgttggg cgatctcgcg ctggtgtggg ccgacgacat
     1321 tgtcgccacc gctgacctgc ccgccgacgc aaggttgcgg gtgcggcggg tctggtcggc
     1381 gatccgaacc gaggtgctcg gtgggcagta cctcgacatc gtcgccgaat ccagcggcgc
     1441 ggaatcggtg gcgtcggcga tgacggtcaa catctacaag accgcgtcat acacgatctc
     1501 ccggccgttg cagttcggcg cctcggccgc cgccgaccgc cccgacgtgc tcgccgcgtt
     1561 ccacgagctc gggaccagcc ttggtgtggc gttccagctg cgcgacgacg tgctcggcgt
     1621 attcggggac ccggcggtca cgggcaagcc gtccggcgac gacctgcgat ccggtaagcg
     1681 cacggtgttg ttggccgagg ccgttgagcg ggccgaaaag gtagaccccg tcgcggccaa
     1741 gctgttacgc acgtcgatcg gcaccgagct gaccgacgct gcggtgaagg aactctgtct
     1801 ggtcatcgaa tcggtcgggg cgctggccgc ggtcgaggag cgtatcgatg cacttacccg
     1861 ccgctcgctg gagatactga acgccgcgtc gatcgacgtc caggccaagg tcggcctgtc
     1921 cgagctcgcc agattggccg cgaaccggtc cgcctaggag catgacaacc ccgacgccga
     1981 cgcaggtccc gaaaaccggg ccggcgcacc atctttggag actgacgtca ttcgcgacgt
     2041 cgccgcaggc acggccagcg ctgctgggcg cactgggtgc cgtgatgatc accgcaggcg
     2101 ggctcggcgc gggcagcacc cggctgcacg atccgctgct ggaatcgctg cacgtgtcgt
     2161 ggcttcggtt cggtcacggc ctcgtgctgt cgtcggtgct tttgtggata ggcgtcgcac
     2221 tgatgctgat tgcctggctg tggttgggcc ggcgggtgat cgaccgcacc gcaaacgaat
     2281 acacgatggt ggcgacgacg gggttctggc tggccccgct gttgctttcc gttcctgtgt
     2341 tcagtcgcga cacctactcg tatctggcgc agggcgcgct gctgcgcgac ggcctcgacc
     2401 cgtacgtcgt cggtcccatc gacaatccga actcgttgtt ggacaacgtg agtccgatct
     2461 ggacaacgac gaccgcgccg tacgggcccg cattcatcct gatcgccaag ttcgtcacga
     2521 tgctggtggg caacgacgtg gtggcgggca ccatgctgtt gcggctgtgc atgctgcccg
     2581 gcctggcctt gctgatctgg gcggcaccgc ggatggcccg ccatgtcggt gccgacggtg
     2641 cggccgcgct gtggatctgc gcgctgaatc cactggtgat cattcatttg atgggtggcg
     2701 tgcacaacga gatgctgatg gtcgggttga tggtggcagg catcgcgctg accttcggcg
     2761 gccaccacat ctggggcgtc ggcttgatcg ctgtcgcagt ggccgtgaaa gcgactgcgg
     2821 gactggctct tccgttcatg gtgtgggtat ggatgcgtca ccttcgcgac ggtcgcggat
     2881 actctccgac gaaggccttc gcagcggcga cggcggcgtc ggcggtgatc ttcgtcgcgg
     2941 tgttcgcggt gttgtcatgg ctggccggag ttgggctggg ctggctgacc gcattggccg
     3001 gctccgtgaa gatcatcaac tggctgaccg tgcccacggc cgcggcgaat ctggtcaacg
     3061 tgttcggcag cctgctgttg ccggtgaact tctacgccgt gctcgatatc acccggatca
     3121 tcgggatcgc cgtcatcgcg atctcacttc ccttggtgtg gtggcggttt cgccacaccg
     3181 accgcgaggc gctgatcggc atcgcggtgg tgatgatcgt ggtggtgctg ttcgtccccg
     3241 ccgcgctgcc gtggtactac acatggccgt tggcggtggt ggccgcgcta gcgcagacgc
     3301 ggcaggcgat cgcggtgatc gcgggcctgt cgacatggat cacggtgatc tggaaacccg
     3361 atggtgcgca cggcatgtac tcatggatcc atgtgctgct cgccacggca tgcgctgcgg
     3421 tggcgtggta ttcgctctac agcgcgccta atacgccatc gcctgggccc gacgcaccac
     3481 ttcccgggcc tgatgcgagt gcagcgcgtc caccggccgg gcattagcct gccgatcggt
     3541 cgtgccgtcg cgtctgatgg tcaatgacgg gtcgggcgtg aacaaccagc gcacgatctc
     3601 ggtgtcgcgg tagccgccat cgtgtagcac tacgagcagg ccggccaggc tcttcaccac
     3661 gtggccggtg tcgtcgaaga aggccttggg caccaccacc gcgccctcgc ggcgcaccgc
     3721 gacgaggtgc ccttcccgca gctgctgttg caccttcgtg acgggaaccc cgagcaaatc
     3781 ggcaacggaa ggtaggtcgt agacggcctc gccggggtcg agaacgtcgt cggcggccgg
     3841 aatgctgctc accgcactga gtctaagacg aaagggacgg ctcgtaacat gtcttcggtg
     3901 gaggcctacc agcaatctga cccgctcgtg ggggccgtgc tggatggccg gtaccgcgtc
     3961 gaggcgatga tcgcgacggg cggcatgtct gccgtctacc gcgggctcga cctgaggctg
     4021 gaccggccgg tcgcgctgaa gatcatggac tcccggtatg ccggcgacaa ccagttcctg
     4081 acccggttcc agcgtgaggc gcgagtggtg gctcgactga aggatcccgg tcttgtcgcg
     4141 gtgtacgacc agggcgtcga cggccagcat ccgtttctgg tgatggagct catcgagggc
     4201 ggcacgctgc gcgagttgct gcgtgagcgc ggtccgatgc cgccgcacgc cgtcgccgca
     4261 gtgctgcgcc ccgtgctcag cggcctggct gtggcacatc aggcgggact ggtgcaccgc
     4321 gatatcaagc ccgagaacgt gttgatctcc gacgacggtg acgtcaagat cgtcgatttc
     4381 ggtttggtgc gggcggtggc cgaagccaag atcacctcca ccagcgtgat tttgggcacc
     4441 gcggcgtacc tgtcccccga gcaggtcagc accggcgacg ccgggccgcg cagcgatgtg
     4501 tactcggtcg gcatcctggc ctacgagctg ctgaccggca ccacaccctt caccggcgac
     4561 tccgcgctcg cggtcgcata ccagcggatg gacaa
//
LOCUS       DASRJA010000185        10367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1690366,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000185 DASRJA010000000
VERSION     DASRJA010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690366"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFC01_08370"
     CDS             <1..544
                     /locus_tag="VFC01_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN79687.1"
                     /translation="GARFLFERKAPYRHEPDLVGRIGYRELNYAAVPDGKPLDPAVAY
                     HEIAADTAEFETEPFDLSDEMPRFTWPTVVISGGRDLITPPAVARRIASLIPGAVLVN
                     LPTAGHSVIDLRERAALEIIKATYDGTSAGLPARAKKLDSTPGGLGVRALVWGIAAAA
                     ALEHALPAVVPRTVARVTAS"
     gene            complement(531..2201)
                     /locus_tag="VFC01_08375"
     CDS             complement(531..2201)
                     /locus_tag="VFC01_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HZN79688.1"
                     /translation="MKIRRFAVIALVAGLTLTACSGGKQETPSAGGSAEVGTTNDINP
                     QDVSKLQQGGNLRLALTAFPENFNSLHIDGNSADSAGMLKASMPRAFRVAADGSMTLN
                     TDYFTGAELTGTNPQVVTWTINPKAFWSDGSPITWEDIASQIHATSGQDKAFAAASPN
                     GADRVESVTRGVDDRQAIMKFAKPYAEWRGMLSGNTMLLPKSMTATPEAFNKGQLSGP
                     GPSAGPFIVSTVDRTAQRIVLTRNPKWWGTPALLDSVTYLVLDDAARIPALQNNTIDA
                     TGLATLDELTIARGTNGVSIRRAPGNSWYHFTFNGAPGSILADKALRLAIAKAIDRQT
                     IANVTQRGLADKPVPLNNHVYVAGQEGYQDNSAVVAFDPEKAKQELDALGWRLNGQFR
                     EKDGRQLVIRDVLYDSLATRQFGQIAQNNLAQVGVKFDLDAKGGAGFFSNHINVGDFD
                     IAQFSWVGDAFPLSGLPQIYASDGESNFGKIGSPEIDAKIEQTLEELDPAKARDLANE
                     VDKLIWAEGFSLPLTQSPGNVAVRSTLANFGAFGLADADYTKIGFMKQ"
     gene            complement(2213..4048)
                     /locus_tag="VFC01_08380"
     CDS             complement(2213..4048)
                     /locus_tag="VFC01_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN79689.1"
                     /translation="MSLLQVTNLSVVFPTDAERVNAVRGMTFHVEPGEVVALVGESGS
                     GKSATATAIIGLLPEYAEVSGSIRLHDDELLGLPDSRMSQIRGKSIGTVFQDPMSALT
                     PVYTVGDQIAEALEIHNRDVTRRDARTRAVELLELVGIAQPERRASSFPHELSGGERQ
                     RVVIAIAIANDPDLLICDEPTTALDVTVQAQILEVLKTARDVTGAGVLIITHDLGVVA
                     EFADRALVMYAGRAVETADVGSLYRDRRMPYTAGLLGSVPRLDAPQGARLVPIPGAPP
                     SMAALPPGCPFAPRCPLAIDDCRAAEPELIPVGPDHRAACIRTDQVAGRSAAEIYGVS
                     TEPPADIQDADASVVLRVRDLVKTYKLTKGVVLRRQVGEVRAVDGVSFELQQGRTLGI
                     VGESGSGKSTTLHQILELSAPQSGSIEVLGEDVGGLDRHGRRALRGDIQVVFQDPVAS
                     LDPRLPVFDVLAEPLQANGFDKNRTDERVAELLNIVGLRREDASRYPAEFSGGQKQRI
                     GIARALALQPKIVALDEPVSALDVSIQAGIINLLLDLQQRFGLSYLFVSHDLSVVKHL
                     AHRVAVMHKGKIVEQGDGDQVFTHPQQDYTRRLLAAVPQPDPNRR"
     gene            complement(4045..4974)
                     /locus_tag="VFC01_08385"
     CDS             complement(4045..4974)
                     /locus_tag="VFC01_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN79690.1"
                     /translation="MTEPVTTDTATTAKSGVDVAAFASRRTLVLRRFRRNKPAVVALL
                     LLVALFIGCYALPPLLPYSYTDLDFYALQQPPSTDHWFGTNPLGQDLLAQTLRGMQKS
                     MLIGVCVAFFSTIIAATVGAIAGYFGGRRDRALMWIVDLLLVVPSFYIIAIITPRTKE
                     SGTVLWLILLLSVFGWMISSRVIRGMTMSLREREFVSAARYMGVSNTRIIIRHIVPNV
                     ASFLIIDTALNVGIAIMAETGLSFLGFGVQPPDVSLGTLINDGKTSVTTFPWVFLVPA
                     SVLVLIILCANLVGDGLRDALDPSARPLRRRRR"
     gene            complement(4971..5948)
                     /locus_tag="VFC01_08390"
     CDS             complement(4971..5948)
                     /locus_tag="VFC01_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN79691.1"
                     /translation="MTRFLARRLLNYIVLLALASFLTFSLASFMFRPLDSLLQRNPRP
                     PQAVIDAKAAELDLDKPILVRYGHWVGGAVHGDFGTTIGGQPVSDELGRRIGVTLRLL
                     VIGSVLGTVIGVVVGAWGAIRQYHLSDRVITVLSLLVISIPVFVLANLLILAALRVNS
                     VFGVQVFEYIGETSPDAVGGAWNQFVDRVQHLVLPTFTLALGGIAGYSRYQRNAMLDV
                     LGQDFIRTARAKGLTRRRALFKHGLRTALIPMATLFAYNIALLVTGALFVETIFGWHG
                     MGEWAVKGIATQDINIVVAIGFFSAVMVLLAGLLSDVIYAALDPRVRIT"
     gene            6165..6428
                     /locus_tag="VFC01_08395"
     CDS             6165..6428
                     /locus_tag="VFC01_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79692.1"
                     /translation="MNSLKRNNVKLLAAVIGGSGVIAMGAMSVAIAQEQAGQESAAKS
                     TTMTVGSTSTETTPPTVEATTMAAPALRGPAALPAEEQGPAAP"
     gene            6593..6835
                     /locus_tag="VFC01_08400"
     CDS             6593..6835
                     /locus_tag="VFC01_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79693.1"
                     /translation="MQATRRSNVKMLAVVIGGSAVVAAGALTMAIGQEQASPATVTSS
                     GMTVGATSTQETPPAVPETTLAVPAVKATPFGGAGS"
     gene            6955..7191
                     /locus_tag="VFC01_08405"
     CDS             6955..7191
                     /locus_tag="VFC01_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79694.1"
                     /translation="MKSSQLKAVAAGVGGLALVAMGALTVVFSESPGNISGAGLSLTL
                     GETTTSETGVTELATSFAVPEVTASVPEGFGNGG"
     gene            complement(7198..7689)
                     /locus_tag="VFC01_08410"
     CDS             complement(7198..7689)
                     /locus_tag="VFC01_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79695.1"
                     /translation="MTTTTGWRWRETAGAVAIAAVTAALGGAAIYAATEGNSHTFGPS
                     HQSFGPGGGKPGGQHGAVGGPGPASVDATSLHGEFVVPDGAGGYTTVVTQTGAVTAIS
                     PTSITVRSDDGYSQAYVIPDTAGNANVPFAVDDHVVVRATRNGQTVTVTNIGANEPPT
                     DSN"
     gene            7876..8580
                     /locus_tag="VFC01_08415"
     CDS             7876..8580
                     /locus_tag="VFC01_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN79696.1"
                     /translation="MRRADGNPIHVLVVDDEPVLAELVSMALRYEGWEIATAGDGASA
                     IASAKDTPPDVVVLDVMLPDMSGLDVLHKLREQIPGLPLLLLTAKDSVEDRIAGLTAG
                     GDDYVTKPFSLEEVVLRLRALLRRTGVTSETGGAKIVVGDLVLDEDSHEVTRGGELIS
                     LTATEFELLRFMMRNAKRVLSKAQILDRVWSYDFGGRSNIVELYVSYLRKKIDAGREP
                     MIHTLRGAGYVLKPPR"
     gene            8558..10156
                     /locus_tag="VFC01_08420"
     CDS             8558..10156
                     /locus_tag="VFC01_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN79697.1"
                     /translation="MSSSPRADAARRRFRSPRSWSLRARLLASQIVLLALVCAAIGVG
                     TELALQRFLMNQLDDRLAETGHRSVGLFEFGPPPPPGMGPMMPGYPDPPGFPRRVIIR
                     DDYGPGPAFLNAPGQAARTVGAVISHGTPVDAGVITADGTRAQISSTAAQQLAHITPD
                     RVPKTVELDGLGRYRVVAMHARGPGETIVTGLPTSEVDDTLLSVLVIFCVIGAVALIG
                     GTTVGIVIIRRQLAPLSRASEAARQVADLELDRGEVRLPTPIVKVDPASADTEIGQLG
                     SALNRMLDRIAGALSARHASETRVRQFVADASHELRTPLAAIRGYTELAQRKRDDLPD
                     DVAHAMSRVESETERMTQLVEDMLLLARLDTGRPLERVQVDLSRLVVDAVSDAHIAGP
                     DHQWLLDLPDEPVLATGDEARLHQVLANLLANARTHTPPGTSVTTSLAVDDSGGAVLT
                     VTDDGPGIPPWLQPEIFERFARGDTSRSRRGGSTGLGLAIVAAVVRAHGGSIDVRSVP
                     GKTEFVVRFPANSQWSHSASKTGA"
     gene            10180..>10367
                     /locus_tag="VFC01_08425"
     CDS             10180..>10367
                     /locus_tag="VFC01_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79698.1"
                     /translation="MTALVLPDEQTQKSPTRRENGDFRVPLLARGPCSRLGLGLLLAA
                     TAVLYLWNLSASGWANAF"
BASE COUNT         1806 a         3372 c         3408 g         1781 t
ORIGIN      
        1 gggcgcccga ttcctattcg aacgcaaggc gccgtaccgt cacgagccgg atctggtcgg
       61 caggatcgga taccgcgaac tgaactacgc cgcggtcccc gacggaaagc cgctcgatcc
      121 cgccgtcgcg tatcacgaaa tagcagcaga tacagcggaa ttcgagaccg aaccgttcga
      181 cctcagtgat gagatgccca ggttcacctg gcctacggtc gtgatttccg gtggccgcga
      241 tctgatcacc ccgcccgcgg tcgcccgcag gatcgcgtca ctgatcccgg gcgcggtact
      301 ggtcaacctc cccaccgccg gacactccgt catcgacctt cgcgagcgtg cggcgctgga
      361 gatcatcaag gccacctacg acggaacgag cgcgggtctg cccgcgcgcg ccaagaagct
      421 ggactcgacg ccgggtggcc tcggggtgcg ggcgctggtc tggggcatcg cggccgcggc
      481 cgcgctcgaa cacgcgctgc cagcggtggt gccccgcacc gttgcgcgcg ttactgcttc
      541 atgaagccga tcttggtgta gtccgcatcg gccagaccga atgcgccgaa gttcgccaaa
      601 gtgcttcgca ccgcgacgtt tccgggcgac tgggtcagtg gcaggctgaa gccctcggcc
      661 cagatcagct tgtccacctc gttggccaga tcgcgggcct tggcggggtc gagctcttca
      721 agtgtctgct cgatcttggc gtctatctcc ggactgccga tcttgccaaa gttgctttcg
      781 ccgtccgagg cgtagatctg gggcagcccc gacaacggga atgcgtcgcc cacccaggag
      841 aattgggcga tatcgaagtc gccgacgttg atgtgattcg agaagaatcc agcgccgccc
      901 ttggcgtcga gatcgaattt gacgccgacc tgggcaaggt tgttctgcgc gatctgcccg
      961 aactggcggg tggccaatga gtcgtacagc acgtcgcgga tgacgagctg gcgtccgtcc
     1021 ttctcgcgga attgcccgtt cagtcgccag ccgagcgcgt ccaattcctg cttggccttt
     1081 tcggggtcaa aggcgaccac ggcactgttg tcctgatagc cctcctgacc ggcgacgtag
     1141 acgtggttgt tcagcgggac gggtttgtcc gcgagcccgc gctgggtgac attggcgatc
     1201 gtctgacgat caatggcctt ggcgatcgcc agccgcagcg ccttgtcggc cagaatcgaa
     1261 ccgggtgcac cgttgaatgt gaagtgatac caactgtttc cgggcgcacg ccgaatcgac
     1321 accccgttgg tgccccgcgc gatggtcagc tcgtccaggg tggccagccc ggtggcgtcg
     1381 attgtgttgt tctgcagggc ggggattcgc gcggcgtcgt cgagcaccag ataggtgacg
     1441 ctgtccagca gcgcaggggt tccccaccat ttcgggttgc gcgtcagcac aattcgctgc
     1501 gccgtgcgat cgacagtgga gacgatgaac ggtcccgccg atgggccggg accgctgagc
     1561 tgacccttgt tgaacgcctc aggcgtcgcg gtcatgctct tcggcagcag catcgtgttg
     1621 cccgacaaca tgccgcgcca ttccgcatac ggtttcgcga acttcatgat cgcctggcgg
     1681 tcatcgacgc cgcgagtcac cgactcgacg cggtccgctc cgttgggact ggcggccgca
     1741 aatgccttgt cctggccact ggtggcgtgg atttgggagg caatgtcctc ccacgtgatc
     1801 ggggagccgt cgctccagaa cgctttgggg ttgatggtcc atgtgaccac ctgcgggttg
     1861 gtgccggtca gctcggcgcc ggtgaagtag tccgtgttca gcgtcatcga cccgtcggcg
     1921 gcgacgcgga acgcccgcgg catgctggct ttcagcatgc ccgccgagtc agcggaattg
     1981 ccgtcgatat gcagcgagtt gaagttctcg ggaaacgcgg tgagcgccag ccgcagattg
     2041 ccgccctgct gcagcttcga cacatcctgc gggttgatgt cattggtcgt gccgacctcc
     2101 gcggacccgc ctgccgacgg ggtctcctgc ttgcccccgg agcacgcggt aagcgtcaga
     2161 cctgcgacaa gggcaatcac ggcgaagcgt cggatcttca tggcgacaga cgttacctgc
     2221 gattcgggtc tggttgagga actgcggcaa gcaatcggcg ggtgtagtcc tgctgcggat
     2281 gggtgaacac ctggtcgccg tcaccttgct cgacgatctt gcccttgtgc atcacggcga
     2341 cccggtgggc gaggtgtttg acgaccgaca ggtcgtgcga cacgaagaga taggacaggc
     2401 cgaaccgctg ctgcaggtcc agcagcaggt tgatgatgcc tgcctggatc gaaacgtcga
     2461 gtgccgagac aggttcgtcc agagcgacga tcttcggctg cagcgccagc gcccgcgcaa
     2521 tgccgatgcg ctgcttctgc ccaccggaga attcggcggg ataccgactg gcgtcctcgc
     2581 ggcgcagtcc gacgatgttc agcagttcgg ctacccgttc atcggtgcgg ttcttgtcga
     2641 agccgttggc ctgcaacggt tccgcgagga cgtcgaacac cggcagccgc ggatcaagcg
     2701 acgcgacggg gtcctggaat accacctgaa tgtccccgcg caacgcgcgt ctgccatgcc
     2761 gatcaagccc gccgacgtcc tcgccgagca cctcgatcga gcccgactgc ggagccgaca
     2821 attccaggat ctggtgcagc gtcgtggatt tcccggagcc ggattcgccg acgatgccca
     2881 gcgtgcggcc ctgctgtagt tcgaagctca caccgtcgac cgcgcgcacc tcgccgacct
     2941 ggcggcgaag caccacgccc ttggtcagct tgtacgtctt caccagatcg cgcacccgca
     3001 gcacaaccga cgcatcggcg tcctggatgt cagccggtgg ctcggtggag acgccgtaga
     3061 tctccgccgc gctgcggccg gccacctggt cggtgcggat gcacgccgcc cggtgatcgg
     3121 ggccgacggg gatcagctcg ggttcggcgg cgcggcagtc gtcgatggcg agcgggcagc
     3181 gtggcgcaaa cgggcaaccc ggcggtagcg ctgccattga cggcggcgct ccgggaatcg
     3241 gcaccaatcg ggcgccctgc ggcgcgtcca gccgcggtac cgagccgagt aaccctgcgg
     3301 tgtaaggcat tcggcggtcg cggtacagcg agcctacgtc ggccgtctcg acggcgcgtc
     3361 cggcgtacat caccagtgcg cggtcggcga attcggcgac cacgccgagg tcgtgggtga
     3421 tgatcagcac gcccgccccc gttacgtcgc gcgcggtctt gagtacctcg aggatttgcg
     3481 cctgcacggt gacgtccagc gcggtggtcg gctcgtcaca gatcagcagg tcgggatcgt
     3541 tggcgatggc gatcgcgatg accacgcgtt ggcgttctcc gccggagagc tcgtgtggaa
     3601 acgagctggc gcggcgctcc ggctgcgcga ttccgaccag ctcgaggagt tcgacagcgc
     3661 gggtgcgggc gtcccgcctg gtcacgtcgc ggttatggat ttcgagcgct tcggcgatct
     3721 ggtcgcccac ggtgtagacc ggcgtgagcg ccgacatcgg gtcctggaac acggtgccga
     3781 tggatttgcc gcggatctgt gacatccggc tgtcgggcaa tccaagcagc tcgtcgtcgt
     3841 gcagccggat cgacccagac acctcggcgt attccggcag cagcccgatg atggccgtcg
     3901 cggtggccga cttgcccgat ccggattcgc cgacgagtgc gacgacctca ccgggctcga
     3961 cgtgaaaagt catgccgcgg acggcattaa cacgctctgc atcggtcggg aacacaacag
     4021 aaaggttggt tacctgcagc aggctcatcg ccgacgcctc cgcagtggcc gagcgctggg
     4081 atccagcgcg tcgcgtaggc cgtcgccgac gaggttggcg cacaagatga tcaacaccag
     4141 cacgctcgcc gggaccagga agacccacgg aaacgtcgtc accgatgtct tgccgtcgtt
     4201 gatcaacgtg ccaagcgata cgtcaggcgg ctgcacgccg aaacccaaga agctcagccc
     4261 ggtttccgcc atgatcgcta taccgacgtt cagcgcggtg tcgatgatga gaaacgacgc
     4321 cacattgggc acgatgtgcc ggatgatgat acgcgtgttg ctcacaccca tatagcgtgc
     4381 agcggagacg aattcgcgtt cccgcagact catggtcatg ccgcgtatga cccgggagct
     4441 gatcatccaa ccgaacaccg acagcagcaa gatcaaccac aggacggtcc ctgactcttt
     4501 cgtccgcggt gtgatgatcg cgatgatgta gaagctgggc accaccaaca acagatcgac
     4561 gatccacatc agcgcgcggt cacgccggcc tccgaagtac cccgcgatcg cccccaccgt
     4621 cgccgcgatg atggtggaga agaacgccac gcataccccg atgagcatgg atttctgcat
     4681 gccgcgcagg gtttgggcca gcaggtcttg cccgagcggg ttggtgccga accagtggtc
     4741 ggtgctcggc ggttgctgca gagcgtagaa atccagatcg gtgtacgagt agggcagcag
     4801 tggcggcaac gcgtagcagc cgatgaagag tgccactaac agcagcaggg cgaccacggc
     4861 gggcttgttg cgccgaaatc ggcgcagcac caacgttcgt cgggaggcga acgcggcaac
     4921 gtcgacgccg gacttggcgg tggttgcggt gtcggtggtc acgggttcgg tcatgtgatt
     4981 cggacccttg ggtcgagcgc ggcgtagatg acgtcggaca gcagcccggc caacaggacc
     5041 atcacggcgc tgaagaagcc gattgcgacg acgatgttga tgtcctgggt ggcgatgccc
     5101 ttgacagccc attcacccat gccatgccag ccgaagatcg tctcgacgaa caatgcgccc
     5161 gtcaccaaaa gggcgatgtt gtaagcgaac agcgtcgcca tcggaatcag cgcagtgcgt
     5221 aggccgtgtt tgaaaagcgc gcggcgccgg gtcagcccct tggcccgtgc ggtgcggatg
     5281 aaatcctggc cgagcacatc gagcatggcg ttgcgttgat accggctgta accggcgatg
     5341 ccgcccagcg ccagcgtgaa tgtcggcaac accaaatgct gcactcgatc gacgaattgg
     5401 ttccaggcgc cgccgaccgc atccggcgac gtctcgccga tgtattcgaa cacctgaacc
     5461 ccgaacaccg agttcacccg cagcgcggca agtatcaaca gattggcgag cacgaacacc
     5521 gggatgctga tcaccaacag cgacaacacc gtgatgacgc ggtcggagag atggtattgc
     5581 cggatcgccc cccacgcgcc gactacgacg ccgatcaccg tgcccaggac cgacccgatg
     5641 accagtagcc gcagcgtcac gccgatgcgc cgcccgagct cgtccgacac gggttgaccg
     5701 ccgatcgtcg tgccgaagtc gccgtgcacc gcgccgccca cccaatggcc gtagcgcacc
     5761 agtatcggct tgtcgaggtc gagttcggcg gccttggcgt cgatgactgc ctgcggtggc
     5821 cgtggattgc gttgcaggag gctgtccagc ggtcggaaca taaaagatgc cagcgagaac
     5881 gtcaggaacg acgccagcgc cagcagcacg atgtagttca gcagccgccg cgcgaggaag
     5941 cgcgtcatcg gcgacatccc tgggcggccc gcatgacgca cagggtatga gatgtctccc
     6001 gcgccagcgc gttcttgcag ccccggcgtg gcaagacaat tcctaccggc gcttaaggcc
     6061 gctaccagtt tgggggagtt gactggctct cacctgcagg tttaccggtc gccgtaaggg
     6121 cgcggcggca tggacaaacg ccaggaaggt gaggtgttcg aggaatgaat tctcttaaac
     6181 gcaacaacgt gaaactgctt gccgcggtga tcggcggcag cggcgtgatc gccatggggg
     6241 cgatgtccgt tgccatcgct caggaacagg cgggccagga gtctgccgcc aagtcgacga
     6301 cgatgaccgt cggctcgaca agcaccgaga cgacgccgcc gacggtggag gccaccacga
     6361 tggcggcgcc ggctttgcgg ggcccggcgg cgttaccggc cgaggagcag gggcccgccg
     6421 cgccgtagcc ttctccgacc caaattcaca ggtgagcgct gtaccagcgc gcacctaggt
     6481 agttcgtacc cctgatccag ggttttcaca gatttgccgc attcactagc tcctacctgc
     6541 acgtttgccg gttcgccgct cgaaccggct caccacgaga ggagcaaaca caatgcaagc
     6601 aaccaggcgg agcaatgtga agatgctggc ggtcgtgatc ggcggcagcg ccgtcgtggc
     6661 cgccggcgcc ctgaccatgg cgatcggcca ggagcaggcg tcgccggcga ccgtgaccag
     6721 ttcgggcatg accgtcggcg cgaccagcac tcaggaaacg ccgcctgccg tcccggagac
     6781 cacgctcgcg gtgcccgccg tcaaggcaac ccccttcggc ggcgccggct cctagccgac
     6841 tcctagtccg accaggtttc acctctgatc acttcggtta cccaaaaaag cggagggtct
     6901 acgtcgaccc tcgtgaactg tccgagacga caggaccgaa tggagtggtc agaggtgaaa
     6961 tcgagtcagc tcaaagctgt cgcggcgggt gtcggtgggc tcgcgctcgt tgcgatgggc
     7021 gcgctcaccg ttgtgttcag tgagtcgccc ggaaatatca gcggcgccgg cctcagcctc
     7081 acgcttggcg aaaccaccac gagcgaaacc ggcgttactg aactcgctac gtcgttcgcc
     7141 gtgcccgagg tgacggcctc ggtgcccgag ggctttggca acggcggctg ataaccgcta
     7201 attgctatcc gtcggcggct cattggcgcc gatattggtg acggtcacgg tctgaccgtt
     7261 gcgtgtcgcg cggaccacca cgtggtcgtc cacggcgaat ggcacgtttg cgttcccagc
     7321 cgtgtctgga atgacgtagg cctggctgta gccgtcgtcg cttcgaaccg tgattgacgt
     7381 cggcgagatc gcggtgaccg caccggtctg cgtcaccact gtggtgtagc cgccagcgcc
     7441 gtcgggcacc acgaactcgc catgtagcga cgtcgcatcg acggacgcgg ggccgggccc
     7501 gccgacggcg ccgtgctggc cgcccggctt gccgccacct ggtccgaacg actggtggga
     7561 cggcccgaac gtgtgcgagt tgccctcggt agcggcgtag attgccgccc cgcctagcgc
     7621 ggcggtgacg gccgcgatcg caacggcccc ggcggtttcc cgccatcgcc agccggtggt
     7681 ggtggtcatg cgaccacact gcaagcagtt cttgtgaatc cgctgtgcgc catttcagcg
     7741 gaggctatgt ctgggaacct tcacagccgg ctcatagata gggcacagtc tggacccatg
     7801 ggcgccgcga ataataggcc cgtgtccact cccagcgctt tcacggtgaa cgagaacgac
     7861 tccggacgcg tcgtcatgcg gcgggccgac gggaacccga tccacgtctt ggtcgtcgac
     7921 gacgagccgg tgctcgccga actcgtgtcg atggccctac ggtatgaggg ctgggaaatt
     7981 gcgactgccg gcgacggcgc ttcggcgatc gcatcggcca aagacacccc gcccgatgtc
     8041 gtggtgctcg acgtgatgct gcccgatatg agcggcctgg acgtcctgca taagctgcgc
     8101 gagcagattc ccgggcttcc gctgctactg ctgactgcga aggattctgt cgaggaccgt
     8161 atcgccggcc tgacggcggg cggcgacgac tacgtcacca aacccttcag cctcgaagag
     8221 gtggtgctgc ggctgcgtgc gctgctgcgg cgcaccgggg tcaccagcga aaccggcggg
     8281 gccaagattg tcgtcggcga tctggtgctc gacgaggaca gccacgaagt cactcgtggc
     8341 ggcgagctca tctctttgac cgcgacggaa ttcgagctgc tgcggttcat gatgcgcaat
     8401 gccaaacgag tactgagcaa ggcgcagatc ctggaccggg tgtggagcta cgacttcggc
     8461 gggcggtcca acatcgtcga gctgtacgta tcgtatctgc gcaagaagat cgatgccggg
     8521 cgagagccga tgatccacac cctgcgcggc gcgggttatg tcctcaagcc cccgcgctga
     8581 cgcggcccgc cggcgcttcc gctcgccgcg gagctggtcg ctgcgggccc ggctgctggc
     8641 gtcccagatc gtcctgcttg ccctggtgtg tgcggcgatc ggggtgggca ccgagcttgc
     8701 gctgcaacgc ttcttgatga atcagctcga tgatcggttg gctgaaaccg gtcaccgatc
     8761 ggtcgggctg ttcgaattcg ggcctccacc gccgccgggc atgggcccga tgatgccggg
     8821 ttacccggat cctcccgggt tcccacgacg ggtcatcatt cgcgacgact acggtccggg
     8881 ccccgccttc ctcaacgctc ccgggcaggc cgcacgcacc gtcggcgcgg tgatatcgca
     8941 cggcacgccg gtcgatgccg gtgtcatcac cgccgacggc acccgagccc aaatcagttc
     9001 cacggcagcg caacaactcg ctcacatcac gccggaccgc gtaccgaaaa cagtggagct
     9061 cgacggactg ggccgctacc gcgtcgtcgc gatgcacgca cgcggacccg gcgagacgat
     9121 cgtgacaggg ttgcccacct cggaggtgga cgacacgctg ctgtcggttc ttgtcatctt
     9181 ctgcgtgatc ggggccgtcg cgctgatcgg cggcaccacc gtgggcattg tgatcatccg
     9241 ccgccaactc gccccgctat caagggcttc cgaagctgct cggcaagtgg ccgacctcga
     9301 actggaccgt ggggaagtgc ggctacccac cccaatcgtc aaagtcgatc ctgccagcgc
     9361 ggataccgaa atcgggcagc tcggctcggc gctgaaccgc atgctggacc gcatcgcggg
     9421 tgcgctgtcc gcgcggcacg ccagcgagac acgggtacgc cagttcgtcg ccgacgccag
     9481 ccacgaactg cgtaccccgc tggccgcgat tcgcggttat accgaactgg cgcaacgcaa
     9541 acgggatgac ctccccgacg acgtcgcgca cgcgatgagc cgcgtcgaat ccgaaaccga
     9601 acggatgacg cagctcgtcg aggacatgtt gcttctggcg cggctggaca ccgggcggcc
     9661 gttggaacgc gtgcaggttg atttgtccag gctcgtcgtc gacgccgtga gcgacgccca
     9721 catcgcgggg cccgaccatc agtggttact ggatctgccc gacgaacccg tccttgccac
     9781 cggcgacgaa gcccggctgc accaggtgct cgccaatctg ctcgccaacg ccaggaccca
     9841 cacgccgccc ggcacctcgg tcaccacatc gctggccgtc gacgactccg gcggtgccgt
     9901 cctgacggtt accgacgacg gtccaggaat accgccgtgg ctgcagccgg agattttcga
     9961 gcggttcgcc cgaggcgata cgtcgcgctc ccgccgcggc ggcagcaccg ggctgggcct
    10021 ggccatcgtc gccgcggtcg tcagggccca cggcggctcg atcgacgtgc gcagcgtgcc
    10081 gggcaagacg gagttcgtgg tgaggtttcc ggccaattca cagtggagtc acagcgcgtc
    10141 caaaaccggc gcctagcgcg ctcaacgaaa ctcgccttga tgaccgccct agttctccct
    10201 gacgagcaga cgcaaaagtc cccgacacgc cgcgaaaacg gggactttcg cgttcccctg
    10261 cttgcgcgtg ggccctgctc gcgcttggga ttgggcctgc ttctggccgc gacggccgtg
    10321 ctgtatctgt ggaacctctc ggccagcggg tgggccaacg cgttcta
//
LOCUS       DASRJA010000186         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2692492,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000186 DASRJA010000000
VERSION     DASRJA010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2692492"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /locus_tag="VFC01_08430"
     CDS             <1..136
                     /locus_tag="VFC01_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HZN79699.1"
                     /translation="ADLVGISRVSARRYLEHFLGSGALELRLQYGVGRPERRYRLGGV
                     "
     gene            complement(122..1408)
                     /locus_tag="VFC01_08435"
                     /pseudo
     CDS             complement(122..1408)
                     /locus_tag="VFC01_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380815.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
     gene            complement(1466..>2984)
                     /locus_tag="VFC01_08440"
     CDS             complement(1466..>2984)
                     /locus_tag="VFC01_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZN79700.1"
                     /translation="TDAERCNPSTRLPAWREGNLVEPLVHGATYFDRLATEVEALRSG
                     DHLFFTDWRGDPDQKMREGGPTIREAFSGAAQRGVVVKGLVWRSHLDKFAYSEEENQH
                     LGEAIEQAGGEVLLDQRVRFGGSHHQKLVVLRHPGEPSRDVAFAGGIDLCHSRRDDSS
                     HRGDPQAVRMSKRYGDHPPWHDVQLRLQGPVVGALDMTFRERWNDPAPLDTLSPIAWI
                     EDKLRGADLTPGKLPPQPPDPPRCGPHAVQVLRTYPDAHFEYDFAPRGERSVARGYSK
                     AVRRARRLIYLEDQYLWSKRVAQLFAHALADNPELHLVAVVPRYPDVDGRLALPPNTI
                     GRRQAIDACRDASPDRVHVFDVENHQCTPVYVHAKVCVVDDLWACVGSDNFNRRSWTH
                     DSELSCAVLDDTGAFARDLRLRLMREHLDRAADGSEDGGLDDPDVAVREMVAAADTLE
                     AWHASGRNGPRPPGRLRPHKPERLGLFTRLWAAPAYRMFYDPDGRSYRDRLAGRL"
BASE COUNT          487 a         1099 c          898 g          500 t
ORIGIN      
        1 cgctgacttg gtgggcattt cacgggtcag cgcccgccgc tacctcgagc acttcctcgg
       61 cagcggcgca ttggagctgc ggctgcaata cggcgtcggc cggcccgagc ggcgttaccg
      121 cttaggcggc gtgtaggtcg gccaacgcga ccccgattct gatttcgact tcagtcgcgg
      181 cgaccagtgg tcccccggta ggggtggcgc ggtcgagcga taccgtgcac ccgtcggcgt
      241 cacgaattcc gcggtgtgcg tgccgttttc gtcgcctgaa gtcacctgcc agcccggtgc
      301 ttccttcgca taattgcacc gctcacattc gcccaatcca ttggtggcgc tggtcgggcc
      361 gccgcgattg cgcggtgtgg cgtgatcacg atgccgaatc ggcgcatcac agtaaggcgt
      421 gcgacacgtt cggtcccgaa tcccgatgaa tccggccagc cccctgggaa agaaccgaga
      481 gcgcgactcc atcgccacca acgatccgct cttcggatgc cgatacagtc gccgaagcgt
      541 cgccaacgcc cgttcatcga ccaccgcatc gcgtaccagg cgctgcgcta ccgacgccgg
      601 aatcggcccg tacccgtcga gcaccgcagg cgcgttgtcc tgcccccata gcgactgatc
      661 cgacaacacc tagactcagc gccactggtt cgggctgctc agcaggacgt cccgtgaccc
      721 gctccaccag cgcggcggcc atcacctgtc cacgggatcg atcgtcgaca gtggtatcgg
      781 cggcacgctt taacgccgca tacacaccga ctccttgagc gacaggcaac agcgcggtca
      841 cataggtcat gcagtcgggc gccggccgga tggtcacggt gcggtccgct tcagccttcg
      901 ccgctctatc aacgaccgag tgcgcgtcga ggcgcgcagc gatctccttg gccgccgcgg
      961 tgatcctcgc gtcccccatc ccctccagct tgctgacatc ggtgcacagt tcggcatcca
     1021 agacccgccg gtcctccacc gacaggcagg cagactcacg cgtaatcaac gtggcccgcc
     1081 actccgaaag gcaccgcgct ccagcgcggc caaagtatgg ggcatctcgt gcaccaaggc
     1141 cttggccaaa ccccagatgc cgcccgcccc gcgccgccga atcctggcgg gccagcgcga
     1201 cctcgctggc caccccgcga ccctgtttgg ccttcggcac cccggcggca gcctcgtcgc
     1261 accgacgttt ctcatccaac gttgccgccg ccctcgcctg acctgccgcc gctgccgact
     1321 tcaccctttc caaccaggcg attcgctcga tcaggcccgc ctcatcggcg agcggatcaa
     1381 ccgccaccaa ctcgtccaga tcgaacatgc gttcgagtat gccatgggtc accgacactt
     1441 ttcgtccgtc gcgttgcgct cgttcctaca acctgcccgc gagccggtcg cgatacgacc
     1501 gaccgtcggg gtcgtagaac atccgatagg cgggtgcggc ccacagccga gtgaacaggc
     1561 cgagccgttc gggcttgtgc ggtcgcagcc ggcccggcgg ccgtggcccg ttgcgaccgg
     1621 acgcatgcca tgcctccaac gtgtccgccg cggcaaccat ttcccgcacc gctacatccg
     1681 ggtcgtcgag cccaccgtcc tcgctgccgt cggccgcacg gtccaagtgt tcacgcatca
     1741 gccgcagccg caggtcgcgc gcgaacgcgc ccgtgtcatc gagtaccgca caagataatt
     1801 cgctgtcatg cgtccacgac cggcggttga agttgtcact tccgacgcac gcccacaagt
     1861 cgtccacgac acacaccttc gcgtgcacat acaccggtgt gcactggtga ttctcgacat
     1921 cgaacacgtg aacccggtcc gggctggcat cgcggcacgc atcgatcgcc tgacggcggc
     1981 caatcgtgtt gggcggcaac gccagccgtc cgtcgacatc cgggtaacgc ggcaccaccg
     2041 caacgaggtg tagctcggga ttgtccgcca gtgcatgggc gaacagctgc gcaacccgct
     2101 tcgaccacag atactgatct tccaggtaga tcagccgccg agcccgtcgc accgccttgc
     2161 tgtacccacg cgcgacactg cgttcgccgc gcggcgcgaa gtcgtactcg aaatgtgcgt
     2221 cgggatacgt gcgcaacacc tgcaccgcgt gcggcccgca ccgcggcgga tccggcggct
     2281 gcggcggaag cttgcccggt gtcaagtccg ccccgcgcag cttgtcctcg atccacgcaa
     2341 tcggcgacag ggtgtccagc ggcgccgggt cgttccagcg ttcccggaac gtcatgtcga
     2401 gagcacccac caccggtcct tgcagccgta gttggacgtc gtgccacggt gggtggtcgc
     2461 cgtagcgttt cgacatccgc accgcctgcg ggtccccccg atgcgatgag tcgtcgcgcc
     2521 gcgagtggca cagatcgatg ccgccggcga acgccacatc acgcgacggc tcccccggat
     2581 gacggagcac aaccagcttc tggtggtgtg agccgccgaa ccggacgcgc tgatccagca
     2641 gcacctcacc gccggcctgt tcgatcgcct cgccgaggtg ctgattctct tcctcgctgt
     2701 aggcgaactt gtccagatgg gaacgccaca ccagaccctt gacgaccacg ccgcgctgcg
     2761 ccgcgccgct gaacgcctcg cggatggtcg gcccgccttc gcgcatcttc tggtccgggt
     2821 cgccgcgcca gtccgtgaag aacaggtggt cgcccgagcg cagtgcctcg acctcggttg
     2881 ccaaccggtc aaagtacgtc gcaccgtgga ccaacggttc caccaggttt ccctcgcgcc
     2941 aggcgggcag ccgggttgag ggattgcagc gttcggcgtc tgtg
//
LOCUS       DASRJA010000187         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3906441,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000187 DASRJA010000000
VERSION     DASRJA010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3906441"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2605)
                     /locus_tag="VFC01_08445"
     CDS             complement(<1..>2605)
                     /locus_tag="VFC01_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HZN79701.1"
                     /translation="KTVYAFPGQGIQSKGMGMEVRARSKAARKVWDTADKFTRDTLGF
                     SVLHVVRDNPTSLIASGVHYHHPDGVLFLTQFTQVAMATVAAAQVAEMREQGAFVEGA
                     IACGHSVGEYTALACVSGVYELEGLLEVVFHRGSKMHEIVPRDQHGRSNYRLAAIRPS
                     QINLPDDEVTEFVAEIAERTGEFLQIVNFNLRGSQYAIAGTVRGLEALEEEVERRREI
                     SGGKRSFTLVPGVDVPFHSSVLRVGVADFRRSLERVMPRDADPALIVGKYIPNLVPRP
                     FTLDRDFIQEIRDLVPAEPLDEILADYDTWRNEKPVELCRKVVIELLAWQFASPVRWI
                     ETQDLLFIEEAAGGLGIERFVEIGVKSAPTVAGLAANTLKLPEYSHNTTEVLNSERDA
                     AVLFATDTDPEPELEPEEDDTPDSALEAAPVEAAAPAAPAPAPAAPSGGPRPDDLGYD
                     AADATMALIALSAKMRIDQIEPLDSIESITDGASSRRNQLLVDLGSELNLGAIDGAAE
                     ADLAGLKGQVTKLARTYKPYGPVLSDAINDQLRTVLGPSGKRPAAIAERVKKTWELDE
                     GWVKHVTIEVVLGTREGTSVRGGALGGLHDGALPDAATVDKVVDAAVLAVAGRRGVSV
                     SLPSAGGASGGVVDSAALTEFAEKVTGRGGVLASAARLILGQLGVDTPVSAEETASDA
                     DLIDLVTAELGSDWPRLVAPTFDGRKAVLFDDRWASAREDLAKLWLLDEGDIDTDWAR
                     LSERFEGAGHAVAAQASWWQGKALATGRNIHASLYGRAAAGAENPGKGRYTDEVAVVT
                     GASKGSIAASVVAQLLDGGATVVATTSKLDDDRLAFYRTLYRDNARFDAKLWVVPANM
                     ASYTDIDALVSW"
BASE COUNT          400 a          914 c          881 g          410 t
ORIGIN      
        1 ccacgaaacc agcgcgtcga tgtcggtgta ggacgccatg ttggcgggga ccacccacag
       61 cttcgcgtcg aagcgggcgt tgtcgcggta cagggtgcgg tagaacgcca accggtcgtc
      121 gtcgagcttg gacgtggtcg cgacgaccgt cgcaccgccg tcgagcaact gggccaccac
      181 cgatgcggcg atggatccct ttgacgctcc ggtgacgacc gcgacctcgt cggtgtagcg
      241 acccttgccc gggttttccg caccggccgc ggcacgcccg tacagcgagg cgtggatgtt
      301 gcgtcccgtc gccagcgcct tgccttgcca ccagctggcc tgggcagcca cggcgtggcc
      361 cgcaccctcg aagcgctccg agagccgggc ccagtcggtg tcgatgtcgc cctcgtccaa
      421 cagccacagc ttggcgagat cctcgcgggc actggcccag cggtcgtcga acagcaccgc
      481 cttgcggccg tcgaacgtcg gagccaccaa acgtggccag tccgaaccga gttcggcggt
      541 gaccaggtcg atgaggtcgg cgtcgctggc ggtctcctcg gcgctgaccg gcgtgtccac
      601 gccgagctgg cccaggatca gccgggccgc cgacgccagc acaccaccgc ggcccgtgac
      661 cttctcggcg aactcggtga gtgccgcgga gtccacgaca ccacccgatg caccgccggc
      721 ggacggcagg gacaccgaca caccgcggcg gcctgcgacg gcaaggaccg ccgcgtcaac
      781 gaccttgtcg accgtcgccg cgtccggcag cgcgccgtcg tgcagcccgc cgagcgctcc
      841 accgcgcacg ctggtgccct cgcgggtgcc gagcacgacc tcgatggtca cgtgcttgac
      901 ccagccctcg tcgagttccc acgtcttctt gacgcgctcc gcgatggccg ccggccgctt
      961 gccggatggg ccgagcacag tgcgcagctg gtcgttgatc gcgtcggaaa gcactgggcc
     1021 gtaaggcttg taggtccggg ccagcttggt gacctgtccc ttgaggccgg cgaggtccgc
     1081 ctcagcggcc ccgtcgatgg cgccgaggtt gagctccgag ccgaggtcga ccagcagctg
     1141 gttgcgtcgc gaggacgcac cgtcggtgat cgactcgatg gagtccagtg gctcgatctg
     1201 gtcgatgcgc atcttcgccg acaacgcgat cagcgccatg gtggcgtcgg ctgcgtcgta
     1261 gccgaggtcg tccggccgcg gaccaccgct cggggcagcg ggcgcaggtg ccggagcagc
     1321 cggtgcagcc gcctcgacgg gcgcggcctc gagggccgag tccggcgtgt cgtcctcctc
     1381 cggctccagc tccggttccg gatcggtgtc ggtggcgaac agcaccgcgg cgtcgcgctc
     1441 ggagttcagc acctcagtgg tgttgtgcga atactcgggc agcttcagcg tgttggcggc
     1501 cagccccgca accgtcggcg ccgacttgac gccgatctcg acgaaccgct cgatacccag
     1561 accccctgcg gcctcctcga taaacagcag gtcctgggtc tcgatccagc gcaccgggct
     1621 ggcgaactgc caagccagca actcgatgac gaccttgcgg cacagctcga ccggcttctc
     1681 gttacgccag gtgtcgtagt cggcgagtat ctcgtcgagc ggctcggcgg gcaccagatc
     1741 gcggatctcc tggatgaagt cgcggtcgag cgtgaacggc ctcggcacca ggttcgggat
     1801 gtacttgccg acgatcagtg ccggatccgc atcgcgcggc atgacgcgct ccagtgagcg
     1861 gcggaagtcg gcaacgccga cccgcagcac cgaactgtgg aacggcacgt cgacgccggg
     1921 caccagggtg aacgagcgct tgccgccgct gatctcgcgg cgccgctcga cctcttcctc
     1981 gagcgcttcg agcccgcgca cggttcctgc gatcgcgtac tgcgagccgc gcagattgaa
     2041 gttgacgatc tgcaggaatt cgcccgtgcg ctctgcgatc tcggcgacga attcggtgac
     2101 ctcgtcatcc ggcaggttga tctgcgatgg ccggatggcg gccaaccggt agttcgaccg
     2161 gccgtgctgg tcgcgcggca cgatctcgtg catcttggag ccgcggtgga agaccacctc
     2221 cagcagccct tcgagctcgt agacaccgga cacacaggcc aaggccgtgt actcgccgac
     2281 ggagtggccg caggcgatcg cgccctcgac gaacgcaccc tgctcacgca tctcggccac
     2341 ctgcgcggca gccacggtcg ccatcgcgac ctgagtgaac tgcgtcaaga acagcacacc
     2401 gtcggggtgg tggtagtgca caccggaggc gatcaggctg gtcgggttgt cgcgcaccac
     2461 gtgcagcacc gagaagccca acgtgtcgcg agtgaacttg tcggcggtgt cccacacctt
     2521 gcgggcggcc ttggaccggg cgcggacctc catgcccatg cccttggact ggatgccctg
     2581 acccgggaac gcgtagaccg tcttc
//
LOCUS       DASRJA010000188         6745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1681082,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000188 DASRJA010000000
VERSION     DASRJA010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681082"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1955
                     /locus_tag="VFC01_08450"
     CDS             <1..1955
                     /locus_tag="VFC01_08450"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HZN79702.1"
                     /translation="LGAGGKYFQMFGDNDIQVSLVANPSHLEAVDPVLEGLVRAKQDL
                     LDVGEEAEGSNHFSIVPMMLHGDAAFAGQGVVAETLNLALLRGYRTGGTIHIIVNNQI
                     GFTTSPGDSRSSEYCTDVAKMIGAPIFHVNGDDPEACAWVARLAVDFRQQYKKDVVID
                     MLCYRRRGHNEGDDPSMTQPYMYDVIDTKRGSRKTYTEALIGRGDISMKEAEDALRDY
                     QGQLERVFNEVRELEKHPIAPSASVESDQMVPAGMKTAVDKSLLARIGDAHLAFPDDF
                     NVHPRVKPVLDKRREMAYEGKVDWAFAELLALGSFLAEGKTIRLSGQDTRRGTFTQRH
                     SVIIDRKTGREFTPLQLLTTNSDGTPTGGKFMVYDSALSEYAAVGFEYGYSVGNPDAL
                     VLWEAQFGDFVNGAQSIIDEFISSGEAKWGQLSDVVLLLPHGHEGQGPDHTSARIERF
                     LLLWAEGSMTIAMPSTPANYFHLLRRHGLDGIHRPLIVATPKSMLRNKAAVSDLKDFT
                     EGKFKSVIEEPTYEEGTGDRNHVKRVLLTSGKIYYELVARKAKDGRDDVAIVRIEQLA
                     PLPRRRLKETLDRYPNAQEYFWVQEEPANQGAWPTFGLTLPEVCSELLSGIKRISRRA
                     MSAPSSGSSKVHAVEQQEIIDEAFAP"
     gene            2034..2798
                     /locus_tag="VFC01_08455"
     CDS             2034..2798
                     /locus_tag="VFC01_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN79703.1"
                     /translation="MTAVDLRHHKADYGYDGDYRKVSAPTVATIVGTISAALLASAAR
                     NLVRRKPVAAAVTGTSAAAIVTTAALFIRTTRVGKFEVWAEILKNLRLRGDETLLDMG
                     CGRGAVLLAAAKLLPHGRAIGVDIWRADQTDNSEQNTLRNADIEGVADRVEVRTADIT
                     DLPFDDDSFDVIVSSLVVHNIPTAEARTKAISEAARVLRPGGRLALVDIFFTNHYAKQ
                     LRALGWTDVRRRNLGWRMWWGPGMGTHVITATKPKG"
     gene            2996..3826
                     /locus_tag="VFC01_08460"
     CDS             2996..3826
                     /locus_tag="VFC01_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN79704.1"
                     /translation="MEGFAGKVAVVTGAGSGIGQALAIELARSGASVAISDVNTEGLA
                     VTEERLKAIGAPLKSDRLDVTEREAFELYADAVKEHFGKVNQIYNNAGIAFAGDIEVT
                     EFKDIEKVMDVDFWGVVNGTKVFLPHLIESGDGHVINVSSVFGLFAVPGHAAYNAAKF
                     AVRGFTEALRQEMELAGHPVKVTTVHPGGIKTNIVRNMTAVDSVDKEELTKTFDKRLA
                     NTSPEKAAQIILDGVRKNKARVLVGPDATALDLIVRITGSGYQRLFSTVMSRMVPEAH
                     "
     gene            4009..4404
                     /locus_tag="VFC01_08465"
     CDS             4009..4404
                     /locus_tag="VFC01_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79705.1"
                     /translation="MRYKLLCASGMMAVALVGAAPAYAVPLGGEAECGNSNPMDGPVA
                     TSSPGNAATSPGSVFNEPGVNSVSGGKGNQAYNSAGAPSQYDRACAQVTASGSGTPIQ
                     PTPNATNTNNSRATRDALGVTHVGKGANK"
     gene            complement(4405..5241)
                     /locus_tag="VFC01_08470"
     CDS             complement(4405..5241)
                     /locus_tag="VFC01_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZN79706.1"
                     /translation="MLSRRVALALIALILVGCGGHGGPPSIAVGSTSDPESQLIAHLY
                     AAALRFYGSPAHVQSGPDPISGLDSGEVRVVPGFTGRLLARFDPDATARSDAQVYRAM
                     VSALPEGVAAGDYTTSAEDKPALAITERTADAWGGRDVAGAVRNCEKLKPGALEAAHT
                     PSKVGACAMPKPREFPDNATLFAALRARQINAAWTTTATPNIPSELVALSDKTALIRA
                     ENLVPLYRRNELNEQQVLALNEVAGVLDTGSLADMRRQVAEGKEPGLVADAWLDAHPL
                     GR"
     gene            complement(5250..6434)
                     /locus_tag="VFC01_08475"
     CDS             complement(5250..6434)
                     /locus_tag="VFC01_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZN79707.1"
                     /translation="MTETLPPHLQSPQIVVTDEDIFEAHVGGKLSAELKAPLDTQRAL
                     SIAYTPGVAQVSRAIAADHALAARYTWANRLVAVVSDGTAVLGLGDIGPSAALPVMEG
                     KCALFKAFGGLDSIPIVLDTKDPDEIVETLVRLRPSFGAVNLEDISAPRCFEIERRVI
                     DALDCPVMHDDQHGTAIVVLAALMGATKALDRDMASLRVVVSGVGAAGVACTNILLAA
                     GVSDITVVDTRGILHPGRDGLTPVKAELAGRTNPDGLTGGMAEALKGADMFLGVSGGV
                     VAEDIVATMAPETIVFALSNPDPEIHPDVAARYASVVATGRSDFPNQINNVLAFPGVF
                     RGALDGGARRITERMKVAAAEAIFAVACDDLAPDRIVPSPLDPRVAPEVAAAVAAAAA
                     EL"
     gene            complement(6492..>6745)
                     /locus_tag="VFC01_08480"
     CDS             complement(6492..>6745)
                     /locus_tag="VFC01_08480"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211984.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZN79708.1"
                     /translation="TTDAARDWLLGTPKDDWVSMAVLSDGSYGVPEGLISSFPVTTKD
                     GNWSIVQGLEIDDFSRGRIDKSTAELDDERKAVTELGLI"
BASE COUNT         1232 a         2200 c         2235 g         1078 t
ORIGIN      
        1 acctcggagc cggcggcaag tacttccaga tgttcggcga caacgatatt caggtgtcac
       61 tggttgccaa cccgtctcat ctggaggccg tggacccggt gctcgagggt ctggtccgcg
      121 ccaagcagga tctgctggac gtcggagaag aggccgaggg ttccaaccat ttctcgatcg
      181 tgccgatgat gctgcacggc gacgccgcct tcgcagggca gggcgtggtg gccgagacgc
      241 tgaacctggc gctgctgcgc gggtaccgca ccggcggcac gatccacatc atcgtcaaca
      301 accagatcgg gttcaccaca tcgccgggcg attcccggtc gtcggagtac tgcaccgacg
      361 tcgcgaaaat gattggcgcg ccgatcttcc acgtcaacgg cgacgacccg gaggcgtgcg
      421 cctgggtggc gcggctggcc gtggacttcc ggcagcagta caagaaggac gtcgtcatcg
      481 acatgctgtg ctaccgccgt cgcggacaca acgagggtga tgacccttcg atgacgcagc
      541 cgtatatgta cgacgtcatc gacaccaagc gcgggtctcg caagacctac accgaagcgt
      601 tgatcggccg tggtgacatc tcgatgaaag aggccgagga cgccctgcgt gactaccagg
      661 gacagctcga gcgggtgttc aacgaggtgc gtgagctcga aaagcacccg atcgcgccga
      721 gtgcctcagt cgaatccgat cagatggtgc ccgccgggat gaagacggcg gtggacaagt
      781 cgctgctggc tcgaatcggc gacgcgcact tggcgtttcc cgatgacttc aacgtgcacc
      841 cacgcgtcaa gccggtgctg gataagcgcc gcgagatggc ctacgaaggc aaggtcgact
      901 gggcatttgc ggagctgctg gcgctcggct cattcctggc cgaaggcaag acgattcggc
      961 tgtcgggcca ggacacccgc cgcggcacgt ttactcagcg gcattcggtg atcattgacc
     1021 gcaagaccgg tcgagagttc acgcccctgc agttgctgac caccaacagc gacggcacgc
     1081 ccaccggcgg caagttcatg gtgtacgact ccgcgctgtc ggagtacgcg gcggtgggct
     1141 tcgagtacgg ctactcggtc ggcaacccgg acgcacttgt gttgtgggag gcccagttcg
     1201 gtgatttcgt caacggggcg cagtcgatca tcgacgagtt catctcgtcc ggcgaggcca
     1261 agtggggcca gctctccgac gtggtactgc tgctccccca cggccacgag ggccagggac
     1321 cggaccacac gtcggcccgc atcgagcggt tcctgctgct gtgggccgag ggctcgatga
     1381 cgatcgcgat gccgtcgacg ccggcgaatt acttccacct actgcggcgg cacgggctgg
     1441 acggcattca ccggccattg atcgtggcaa cgccgaagtc gatgctgcgc aacaaggctg
     1501 cggtcagcga cctcaaggac ttcaccgagg gcaagttcaa gtccgtcatc gaggagccca
     1561 cctacgagga gggcaccggc gaccgcaacc acgtcaagcg cgtgctgctg acgagcggca
     1621 agatctacta cgagctcgtc gcgcgcaagg ccaaggacgg gcgtgacgat gtcgcgatcg
     1681 tgcgtatcga acagctcgcc ccgctgccga ggcgacggct caaggagacg cttgaccggt
     1741 atccgaatgc ccaggagtac ttctgggtgc aggaggagcc ggcaaaccag ggcgcatggc
     1801 cgacattcgg gctcactctt ccggaagtgt gctcggagct gttgagcgga atcaagcgga
     1861 tttcgcgtcg cgcgatgtcg gcgccgtcgt cggggtcgtc gaaggtgcat gcggtcgagc
     1921 agcaggagat catcgacgag gcgttcgcgc cgtagctgtc agtggccttg gggcctgctg
     1981 tgcgccaaag acgtagcgcc ccacggccgg tggcggcgat ctaccgtgag tagatgactg
     2041 ccgtagacct tcgccatcac aaggccgatt acgggtacga cggggattac cggaaggtgt
     2101 ccgcgccgac cgtggcgacc atcgttggga ctatcagcgc cgcgctgctc gcgtcggccg
     2161 ccagaaatct tgtgcgccgc aagcctgtcg ccgcggcggt gacgggcacc tcggccgcag
     2221 ccatcgttac gaccgcggcg ctgttcatcc gcaccacgcg agtcggcaag ttcgaggtat
     2281 gggcagaaat cctcaagaac ctcaggctgc gcggtgacga aacgctgctc gacatgggat
     2341 gcggacgtgg cgcggtcctg ctcgccgcgg ccaagctgct tccgcacggc cgcgcgatcg
     2401 gcgtcgacat ctggcgcgcc gaccaaaccg acaactcgga gcagaacacg ctgcgcaacg
     2461 ccgacatcga gggtgtcgcc gaccgcgtcg aggtgcgcac agccgacatc accgatctgc
     2521 cgtttgacga cgacagtttc gacgtgatcg tcagcagcct tgtggtgcac aacattccga
     2581 ctgcggaggc ccgcaccaag gcgatcagcg aagcagcccg agtgctgcgc cctggcggca
     2641 ggctggctct cgtcgacatc tttttcacca atcactacgc caagcagctt cgcgcgcttg
     2701 gctggaccga cgtccgtcgt cgcaacttgg gctggcggat gtggtggggc ccagggatgg
     2761 gcacccacgt catcaccgcg acgaagccga aagggtaagc ctctcgccgg ggctattgcc
     2821 gagatcgaca ccagggctgt ggatcactcg ctgtggcagt ccgcgaacaa ccatggtgtc
     2881 gaaatgggcg ctagcagctg aaggcgcagt actccgcgtc gcgagccccc ctgaaggcgg
     2941 accgcgggta ccgactaacc tcgacgcgag tcaaccgaca cgagggagtg tgcgcatgga
     3001 gggcttcgct ggaaaggtcg cggtggtgac cggcgccgga tcgggcatcg ggcaggcgct
     3061 ggccatcgag ctggcccgct cgggcgccag cgtggcgatc agcgacgtca acaccgaggg
     3121 cttggcggtc accgaggagc ggttgaaggc gatcggcgcg ccgctgaagt ccgaccggct
     3181 ggatgtcacc gagcgcgagg cgttcgagct gtacgccgac gcggtcaagg agcacttcgg
     3241 caaggtcaac cagatctaca acaatgcggg catcgcgttc gccggcgaca tcgaggtcac
     3301 cgagttcaag gacatcgaga aggtgatgga cgtcgacttc tggggcgtcg tcaacggcac
     3361 caaggtgttc ctcccccact tgattgaatc cggcgacggc cacgtcatca acgtctcgag
     3421 tgtcttcggg ctgttcgcgg tgcccggaca cgccgcctac aacgcggcga agttcgcggt
     3481 ccgcggcttc accgaggctc tccgccagga gatggaactg gccgggcatc cggtgaaggt
     3541 gacgaccgtg caccccggcg gcatcaagac caacatcgtg cgcaacatga cggccgtcga
     3601 cagcgtggac aaggaagagc tgacgaagac attcgacaag cggctcgcca acacctcccc
     3661 ggagaaggcc gcgcagatca tcctcgacgg cgtccgcaag aacaaggccc gcgtgctcgt
     3721 cggccccgac gcaacggcgc tcgacctcat cgtccgcatc accggatcgg gctaccagcg
     3781 gctgttctcg actgtgatgt cgcggatggt gcccgaagcg cattgacaac gtcgaaatcg
     3841 ttgtccaaaa agcgaattcg cttccagcgc actcacagta ttgccgcgat gcacatcgct
     3901 tgaaccccac ggccgtcgac ttcgtggttc aagtgagggg gtcgtaacgg gttcaatgat
     3961 gtgtgccccg gacaaccgct agctccgtga ggaacggagg ggaaaaccat gaggtacaaa
     4021 ctgctttgcg cgagcggcat gatggccgtg gccctggtag gtgccgcgcc cgcatacgcc
     4081 gtcccacttg gcggcgaagc cgaatgcggg aacagcaatc ctatggacgg gccagtagcc
     4141 accagttcgc cgggcaacgc cgcgacgtca ccgggttcgg tgttcaacga acccggcgtc
     4201 aacagcgtca gcggcggcaa gggtaatcag gcctacaaca gtgcgggagc gccgtcgcag
     4261 tatgaccgcg cctgcgccca ggtgaccgcg agtggctccg gcacgccgat tcagcccacg
     4321 ccgaatgcca ccaacacgaa caattcgcgg gccacgagag acgccctcgg ggtgacgcac
     4381 gtcgggaagg gcgccaacaa ataactagcg gccgagcggg tgcgcgtcga gccacgcatc
     4441 ggctaccagc cccggttcct tgccctcggc gacctggcga cgcatgtctg ccagcgaccc
     4501 ggtgtcgagc acgcctgcca cctcgttgag agccagcacc tgctgctcgt tcagctcgtt
     4561 gcgccggtat agcggcacga ggttctcggc ccggatcaaa gccgtcttat cggagagcgc
     4621 caccagctcc gaaggaatgt taggcgtggc cgtcgtcgtc caggcagcat tgatctggcg
     4681 agcgcgtagc gcagcgaaaa gtgttgcgtt gtccgggaat tcgcgcggtt tgggcatcgc
     4741 gcacgctccg accttcgacg gcgtgtgcgc cgcctccagc gcgccaggct tcagcttctc
     4801 gcagttgcgg accgccccgg ccacatcgcg cccaccccac gcgtcggcgg tccgctcggt
     4861 gatcgcgagc gcgggcttgt cttccgcgga tgtcgtgtag tcgcctgctg cgacgccttc
     4921 gggcagcgcc gacaccatcg cgcggtacac ctgcgcgtcc gaacgggcag tggcgtcggg
     4981 atcaaacctc gccagcaggc ggccggtgaa tccgggcacg acccgaacct cccctgaatc
     5041 cagcccggag atcggatccg gcccggattg cacgtgtgcc gggctgccgt agaaccgcag
     5101 cgccgcggca tacaggtgcg cgatcaactg cgattccggg tcggatgtgg agccaactgc
     5161 gattgacggc gggccgccgt gaccgccgca gcccacaagg atcagcgcga taagcgccaa
     5221 cgccactcgg cgtgacagca tcgggccgat cagagttcgg cggccgccgc ggccaccgcc
     5281 gccgcgacct ccggtgcgac ccgcggatcc agcgggctcg gcacgatccg gtccggcgcg
     5341 aggtcatcgc acgccaccgc gaatatggct tcggcggcgg ccaccttcat ccgctcggtg
     5401 atccgccggg cacccccgtc cagcgcaccg cggaacacac ccgggaacgc cagcacattg
     5461 ttgatctggt tcgggaagtc actgcggccc gtggccacca ccgaggcgta cctcgcagcg
     5521 acatcgggat ggatctccgg gtcgggattc gacagcgcga acacgatggt ctccggagcc
     5581 atcgtcgcga caatgtcctc ggccaccaca cctccggaga cacccaggaa catgtcggcg
     5641 cccttgagag cctccgccat gccaccggtg aggccgtcgg ggttcgtgcg tcccgccagt
     5701 tcggccttga ccggggtcag cccgtcgcgg ccggggtgca ggatgccgcg ggtatccacc
     5761 acggtgatat ccgaaacccc cgcggccaga agaatattgg tgcatgcgac gccagcggcg
     5821 ccgacaccag agaccaccac gcgcagcgag gccatgtcac ggtcgagcgc cttggtggcg
     5881 cccatcagtg ccgccagcac aacgatcgcc gtgccgtgct ggtcgtcgtg cattaccggg
     5941 cagtcgagcg cgtcgatgac ccgtcgctcg atctcgaagc agcgcggcgc ggagatgtcc
     6001 tcgaggttga ccgctccgaa cgacggacgc aggcgcacca gggtttcgac gatctcgtcg
     6061 ggatccttcg tgtccagcac gatcgggatc gagtccaggc cgccgaacgc cttgaacagt
     6121 gcgcacttgc cctccatcac gggcagggcg gccgaaggac cgatgtcgcc gaggccgagc
     6181 accgcggtgc cgtcgctgac cacggccacc agccggttgg cccaggtata ccgcgcggca
     6241 agggcgtggt cggcggcgat tgcgcggctg acctgcgcca cgcctggggt gtaggcgatc
     6301 gacagcgcgc gttgcgtgtc cagtggggcc ttcagctccg cagacagctt gccgcctacg
     6361 tgggcctcga agatgtcctc atcagtgacg acaatctgcg ggctttgcaa gtgcggcggc
     6421 agcgtttcgg tcaccggctc aggttactgg gcctaattaa cgacttcgcc gacgggctgt
     6481 aaaccccgga ctcagatcag tcccaactcg gtaacagcct tccgctcgtc gtcgagttcg
     6541 gcggtcgact tgtcgatccg gcctcgggag aagtcgtcga tctccagccc ctgcacgatg
     6601 ctccagttgc cgtccttggt ggtcacgggg aacgaggaga tcaggccctc gggcacgccg
     6661 tagctgccgt cggaaaggac ggccatcgac acccagtcgt cctttggggt gccaagcagc
     6721 cagtcccgag cggcgtcggt ggtcg
//
LOCUS       DASRJA010000189         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2408706,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000189 DASRJA010000000
VERSION     DASRJA010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2408706"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2037
                     /locus_tag="VFC01_08485"
     CDS             <1..2037
                     /locus_tag="VFC01_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZN79709.1"
                     /translation="IHTDHLVDPDDTYILDNVVRFAGQRVVAVVADTVAAAEEGCSKV
                     VIEYEPLPAVFDPEEAMTPGAPQLHGSDDPFVRDPERNILLELHGEIGDIEAGFAQAD
                     VVHEGTYFSPRVQHAHLETHGSIAWMEDDRLIVRTSSQSPSIAKVKLCHLFNLRPDQV
                     RVFCKRVGGGFGGKQEVITEDLVALATLDIKRPVCFEYTREEEFTTASPRHPMKATVK
                     LGSRADGTLTAFQFRNVSNTGAYGNHGGETLFAAGAAIAIYRCPNKRFDAYSVYTNSV
                     PSGALRGYGMTQPAFAVESAMDELAIALDLDPLELRRRNIVRPGDALLAIDDHPDDVA
                     FTEDGLGKCIDLVDDALRHHTNGEVLGDDWMVGTGVASSLHETAPPTDHISDAWATLR
                     DDGIYEIAVGTVEFGEGTSTAHVQIAATVLGTTPSRIHLVQSDTDRTGFDTGAFASAG
                     LFVAGNAVQRAATALRDRMLEVAATHMNLAAADCAMDDDAVICGDKRMSLAELIEVGH
                     ARGMRFTEARKAYGSPRSVTSNTHGFRVAVHRITGEIRILYSVQATDAGVVINPVQVR
                     GQVEGGVAQGIGFVLTENFHVDANGVMMNPNLRNYRIPTYADAPRTEVLLVDSADSVG
                     PMRAKGIAECCINPVAPALANALANATGVRYRSLPLSPERIYSRLRDSRLVPTI"
BASE COUNT          403 a          683 c          650 g          335 t
ORIGIN      
        1 atccacaccg accacctcgt cgatcccgac gacacgtaca tcctcgataa cgtcgtgcgg
       61 ttcgccggtc aacgggtggt tgccgtggtc gccgacacgg tggccgctgc cgaagagggc
      121 tgcagtaagg tcgtaatcga atacgagcca ctgccagcgg ttttcgatcc cgaagaggcg
      181 atgacacctg gggctccgca acttcatgga tccgatgacc cgttcgtccg cgatcccgag
      241 cgcaacattc tgctggaact tcacggtgag atcggcgaca tcgaagccgg cttcgcacaa
      301 gccgacgtgg tccacgaagg cacgtacttc tcgccccggg tccagcacgc ccatctggag
      361 acgcacgggt ccatcgcgtg gatggaggac gaccggctga tcgtccgcac aagctcgcag
      421 tcgccgtcga tcgccaaggt caagttgtgc cacctgttca acctgcgccc cgaccaggtc
      481 cgtgtcttct gcaagcgggt cggaggcggc ttcggtggca agcaggaggt gatcaccgag
      541 gatctggtgg cgttggccac tttggacatc aagcgccccg tgtgcttcga atacacccgc
      601 gaagaggagt tcacgacggc ctcaccgcgg cacccgatga aggcaacggt caagctgggc
      661 tcccgcgccg acgggacgct gaccgcgttc caattccgca acgtgtccaa caccggcgca
      721 tacggcaacc acggcggcga gaccttgttc gcggccggcg cggccatcgc catataccgt
      781 tgtccgaaca agagattcga cgcttactcc gtctacacga acagcgtgcc cagtggtgcg
      841 ctgcgcggat acggcatgac ccagccggcg ttcgcggtgg agtcggcgat ggacgaactt
      901 gcgatcgcgc tcgacctcga tccgctggag ctgaggcggc gcaacatcgt gcggccgggg
      961 gacgcgctgc tggcaatcga cgaccatccc gacgacgtcg cgttcaccga agacggcctc
     1021 gggaagtgca tcgatctggt cgacgacgca ctgcgacacc acaccaacgg cgaggtcctc
     1081 ggcgacgact ggatggtggg tacaggggtc gcaagctcgc tgcacgagac agcacctccc
     1141 acagaccaca tctccgacgc ctgggcaact cttcgagacg acgggatcta cgagatagcc
     1201 gtcggcacag tcgaattcgg cgaagggacc tcgaccgcgc acgtccagat cgcggccacc
     1261 gtgctcggga ccacgccgtc gcgaatccac ctggtgcagt cagacaccga ccgcaccggt
     1321 ttcgacacgg gagcattcgc cagcgcaggg ctcttcgtcg caggcaacgc cgtgcagcgg
     1381 gccgcgaccg ccctgcgcga ccggatgctg gaagtcgcgg cgacgcacat gaacctggcc
     1441 gccgcggact gcgcgatgga tgacgacgcc gtcatctgcg gcgacaagcg gatgtccctg
     1501 gccgaactta tcgaggtggg ccacgctcgc ggcatgaggt ttaccgaggc tcgcaaggcg
     1561 tatgggtcac cacgcagcgt cacgtcgaac acccatggct tcagggttgc cgttcaccgg
     1621 atcaccggcg agatccgcat actctacagt gtccaggcca ccgatgccgg tgtcgtgatc
     1681 aaccccgtac aggtgcgcgg acaggtcgaa ggtggtgtcg cgcaagggat tggcttcgtg
     1741 ttgacggaga acttccacgt cgatgcgaac ggcgtgatga tgaacccgaa cctgcgcaac
     1801 tacaggatcc cgacctacgc cgacgctccc cgcaccgaag tgctcctcgt tgactctgct
     1861 gactcggtcg gcccgatgcg cgccaaggga atcgcagagt gctgcatcaa tccggtggcg
     1921 ccggcgttgg ccaacgccct cgcgaacgcg accggtgtgc gttaccgctc gctgcctctg
     1981 tcgcccgaac gaatatacag ccggcttcgg gacagccgat tggttccgac gatctagcgg
     2041 tcgaggcgaa accatgcaaa cacagaagat c
//
LOCUS       DASRJA010000190         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1678479,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000190 DASRJA010000000
VERSION     DASRJA010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678479"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VFC01_08490"
     CDS             complement(<1..751)
                     /locus_tag="VFC01_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN79710.1"
                     /translation="MLLGAIDASAQGRRARLSRDLAQQLESVTDTATCVIVPGTRAQL
                     DGLASRHGLTIRKRLKAGAVVEIPAGALAAVAADAGLDSLTSNYRLQPHMAVTTAAIG
                     ADQVWSTGWVAGAPGLTGSGIGVAVIDTGVAAVPELKNRIVVSVDFTGSSGASGQAVG
                     RAGCGGAGTAVRGRADDDNGHGTHVAGIIAAAGVNAKDTTSGLAPGAHIINLKVLGAD
                     GSGYASDVAEAIDWAVENRDRYKIKVINLSLG"
     gene            complement(853..1113)
                     /locus_tag="VFC01_08495"
     CDS             complement(853..1113)
                     /locus_tag="VFC01_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79711.1"
                     /translation="MATEYSELHAPQRLGRSVSIDQAAQLLSVSRRTVYYRIRDGRLQ
                     TIRTIGGSQRVLVESLFTMGFRPQPFPTSAMAATFTVRAARG"
     gene            1524..>2768
                     /locus_tag="VFC01_08500"
     CDS             1524..>2768
                     /locus_tag="VFC01_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN79712.1"
                     /translation="MASIRAEPRSFNRLVARDQATELLTQLTQGRLVRVNRATFELEP
                     WLAERWESSGDGRAHTFHLRPGVTWSDGTPFTSADVLFSLRAVFDPKSESVLASILVV
                     GGQPIHATAPDPQTVIVTFAGASGPGIRLLDQLPILPKHKLEGALASGKFASAWASTT
                     PPADIVGTGPFVVREYQPGQRVVLDRNTRYWRRAADDGELPYVDRIVLEIVPEQNAEL
                     LRLQSGSTDLTSTELRPEDYVPVRRAEEQGQLRFVELGVGPDADAFWFCLKPEVKGKD
                     PRFAFVQRPEFRRAISHAVDREAFAQTVFLGAAVPVWGPITPGNTQWFSPNVLRYPHD
                     DNRARELLKGIGLEDRNGNGVVEDARGTEARFTGITQRGIGWYERGTAVLRDELAGIG
                     IAIDFEPLEVGALIQRLLACDYD"
BASE COUNT          457 a          896 c          948 g          467 t
ORIGIN      
        1 caccgagcga caggttgatg accttgatct tgtagcggtc gcgattctca accgcccagt
       61 caatcgcctc ggccacgtcg ctggcatagc cgctgccgtc ggcacccagc accttcagat
      121 tgatgatgtg tgctccgggc gccaggccgc tggtggtgtc cttcgcgttg accccggccg
      181 ccgcgatgat gccggccacg tgcgtgccat gaccgttgtc gtcgtcagcg cgtccccgca
      241 ccgccgtccc ggcgccgccg cagccggcgc ggcccaccgc ctggccggac gcgccggacg
      301 acccggtaaa gtccacgctg acgacgattc gatttttgag ctccggcaca gccgcgacgc
      361 ccgtgtcgat cactgcgacg ccgatgccgc tgccggtgag gccgggagca cccgctaccc
      421 agccggtcga ccacacctga tcggcgccaa tcgcggccgt cgtcaccgcc atgtgcggtt
      481 gcagccgata gttgctggtg agggagtcga gccccgcgtc agccgccacg gccgccaggg
      541 cgccggccgg tatctcgacg acggcgcccg ccttgagcct tttgcggatg gtgagtccgt
      601 gtcgcgacgc caatccgtcc agctgcgcgc gcgtgccggg gacgatgacg caggtggccg
      661 tgtcggtgac ggactcgagc tgctgggcaa gatcgcggga gagacgcgcc cggcgccctt
      721 gtgccgaggc gtctatcgcc cccaacagca ccgccgcaag acagatcagg ggaacagcga
      781 aggaggattg tctggtcggt gcggcggtgc tctgtggggc caatgaaacc tccgggaaca
      841 ggaaaaggtc tgtcagcctc gagcggcgcg caccgtgaac gtcgccgcca ttgcggacgt
      901 cggaaacggt tgcggccgga atcccatcgt gaacagcgac tcaacgagca cgcgctgaga
      961 gcctccgatg gtgcggatgg tctggagacg gccgtcgcgg atgcggtaat aaacggtgcg
     1021 tcgcgatacg ctcagcagct gcgcggcctg atcgattgat acgcttcgtc cgagacgctg
     1081 gggagcgtgc aattcggaat attcagttgc cacttaatga gcctccgggc cgttgctatg
     1141 gcagaggcgg tgccagcagc cgggccgaat catgaggcgc gcgtaattcg atgatcgaga
     1201 acgacttatg agctacagcg cagctggttg cgcgcgtcgg gttggcctgc ggcaggctag
     1261 aggtttggcc gcttcaatcg aggaagcgcc cggcgaggcg cactattggg cattgcacgg
     1321 ttctgtgctg aaatctgcac gattttcagc gcggccaata aattgaccgg agcatcaaga
     1381 aactgcgcgt aaaaagttct ctcgtcaggg cggttctggg tgcggcggtg cttgccctcg
     1441 cgctcgggtt cctgttgcgg agcgaaccga gcgattcgcc gtctgttaaa acggatacgc
     1501 cgccaagccg tggcgggcag atcgtggcct ccatccgggc cgagccgcgg tcgttcaacc
     1561 ggctcgttgc ccgcgatcag gccacggaac tcctcacgca gctcacccag ggacgcctgg
     1621 tgcgggtaaa ccgcgccacg ttcgagctgg agccgtggct ggccgagagg tgggaatcga
     1681 gcggcgacgg ccgcgcgcac acgttccacc tccggccggg cgtgacctgg tccgacggca
     1741 cgccctttac gtcggccgac gttctcttct cgctgcgagc cgtcttcgac ccgaagtcgg
     1801 agagcgtgct ggccagcatc ctggtggtcg gcgggcagcc gatccatgcc accgcgcccg
     1861 acccgcaaac ggtgatcgtg acgtttgccg gtgcgtcggg tccagggatc cggctcctcg
     1921 accagctccc gattctgccg aagcacaagc tggagggtgc actcgcctcc ggcaaattcg
     1981 ccagcgcctg ggccagcacc acacctcccg ccgacatcgt cggcaccggg ccgttcgtgg
     2041 tccgcgaata ccagccggga cagcgggtcg tgctggaccg caatacccgc tactggcgcc
     2101 gtgcggcaga cgacggcgag ctgccgtatg tggaccgcat cgttctggaa atcgtgcccg
     2161 aacagaatgc ggagctgctg cggctgcagt cgggatcgac agacctcacg tcaacggagc
     2221 tgcggccgga ggactacgtg ccggtgcgcc gggcagaaga gcaggggcag ttgcggttcg
     2281 tcgagctcgg cgtcggtccc gacgccgacg cgttctggtt ctgcctgaag cccgaggtca
     2341 aggggaagga tccccgtttc gctttcgtgc agcgccccga attccggcgc gccatttcac
     2401 acgcggtcga tcgggaggcg tttgcgcaga cggtgtttct tggcgcggcc gtgccggtgt
     2461 ggggtccgat cacgcccggg aacacgcagt ggttctcccc caacgtgctt cgctaccccc
     2521 acgacgacaa tcgcgctcgt gagctgctga aggggattgg gctggaggat cgcaacggga
     2581 acggcgtggt cgaggacgca cgcggcacag aggcgcgctt caccggtatc acgcagcgcg
     2641 gtatcggctg gtacgaaaga ggcacagcgg tgctgcgcga cgaactggcg ggcataggca
     2701 tcgccatcga tttcgagccg ctcgaggttg gcgcgctcat ccagcggctg ctggcgtgcg
     2761 actacgac
//
LOCUS       DASRJA010000191         8331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2974179,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000191 DASRJA010000000
VERSION     DASRJA010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2974179"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1510
                     /locus_tag="VFC01_08505"
     CDS             98..1510
                     /locus_tag="VFC01_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HZN79713.1"
                     /translation="MAAPSPGYSITVRVEAPASSRATGELTAAIGAAGGALTALDVAE
                     SRHDSIVVDVTCDVADAAHGDTITEAIDALPGFRVRKVSDRTFLMHLGGKLEVVPKVP
                     LRHRDDLSRAYTPGVARICQAIAANPEDARRLTIKRNTVAVVTDGSAVLGLGNLGPAA
                     ALPVMEGKAALFKRFAEVDAWPVCLDTQDTDQIVEIVCALAPVYGGINLEDIAAPRCF
                     EIERRLRERLDIPVFHDDQHGTAIVVLAALMNAVRVVHKKLPDLRVVISGVGAAGSAI
                     IRLLQAQGVQDIVAAGRKGVLSRDEDYADPTRRWIAENTNPRGVTGSLKQALADADVF
                     IGVSAPRLLDGDDIATMADRAIVFALSNPDPEVDPLEARQHAAVVATGRSDFPNQINN
                     VLAFPGFFRGLLDAGAHEINDDMMIAAAKAIAEIVTPEELNPSFIVPSVFDSTVAPAV
                     AAAVSPVASEPDRDSGLRQR"
     gene            complement(1539..1615)
                     /locus_tag="VFC01_08510"
     tRNA            complement(1539..1615)
                     /locus_tag="VFC01_08510"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Pro,seq:ggg)
     gene            complement(1676..2458)
                     /locus_tag="VFC01_08515"
     CDS             complement(1676..2458)
                     /locus_tag="VFC01_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZN79714.1"
                     /translation="MPVSHMILIALAGVGAGAINAIVGSGTLITFPTLVALGYPPVTA
                     TMSNAVGLVAGGVSGTWGYRRELRGQWHRLRWQIPASLVGALLGSWLLLHLPEKVFEK
                     VVPVLLVLALGLVVVGPRIQEWARRRAEESGRSAEHVSGRRMAALVFGTFAIGIYGGY
                     FTAAQGIMLIAAMSALLPEDMQRMNAAKNLLSLLVNIVAAVAYTVVAFDRISWEAAGL
                     IAVGSLIGGFLGAHYGRRLSPNALRAAIVMVGAIGLYRLVTI"
     gene            complement(2501..3901)
                     /gene="der"
                     /locus_tag="VFC01_08520"
     CDS             complement(2501..3901)
                     /gene="der"
                     /locus_tag="VFC01_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZN79715.1"
                     /translation="MSEGTWADESDWELGAEEVAEAIEAAAPPPVVAVVGRPNVGKST
                     LVNRILGRREAVVQDVPGVTRDRVSYDALWSGRRFVVQDTGGWEPDAKGLQQLVAEQA
                     SVAMRTADAIVFVVDATVGATTADEAAARLLQRSGKPVFLAANKVDNERGEADAAELW
                     SLGLGEPHPISAMHGRGVADLLDKVIDALPAVSEMASGGGGPRRVALVGKPNVGKSSL
                     LNRLAGDERSVVHDVAGTTVDPVDSLIEMDGKVWRFVDTAGLRRKVGQASGHEFYASV
                     RTHGAIDAAEVAIVLIDASQPLTEQDQRVLSMVIEAGRALVLAFNKWDMVDEDRRYLL
                     DREIDRELAQLQWAPRVNISAKTGRAVQKLVPALETSLASWDTRVPTGRLNTFFKEIV
                     AATPPPVRGGKQPRILFATQATARPPTFVLFTTGFLEAGYRRFLERRLRETFGFEGSP
                     IRINVRVREKRGAKSR"
     gene            complement(3898..4584)
                     /gene="cmk"
                     /locus_tag="VFC01_08525"
     CDS             complement(3898..4584)
                     /gene="cmk"
                     /locus_tag="VFC01_08525"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZN79716.1"
                     /translation="MSASLVIAVDGPAGTGKSSVSRGLARSLGARYLDTGAMYRIVTL
                     AVLRAGVDLADTPAIETAAADVPLSVGYNPDEDRAYLDAEDVSAEIRGDEVTKAVSAV
                     SAVPAVRARLVGLQRELAAGPGHVVVEGRDIGTVVLPNADVKIFLTASAEERARRRND
                     QNIASGLGDDYEAVLADVKRRDHLDSTRAVSPLRAADDALVVDTGDMTETEVVVHLLE
                     LVEQRAGASR"
     gene            complement(4581..5324)
                     /locus_tag="VFC01_08530"
     CDS             complement(4581..5324)
                     /locus_tag="VFC01_08530"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZN79717.1"
                     /translation="MADSEGVRLQKVLSQAGIASRRVAEKMILDGRVEVDDRIVTELG
                     TRVNPDVSVIRVDGARVTVDDTLVHLAINKPKGMHSTMSDDRGRPCIGDLVEHRVRGN
                     KKLFHVGRLDADTEGLMLLTNDGELAHRLMHPSFEVPKTYLATVDGSVPRGLGRKLRD
                     GIELDDGPAHVDDFALVDKVPGKTLVRVTLHEGRKRIVRRLLAAVGHPVQELVRTDIG
                     SVSLGDQRPGSIRVLSRKEVGELYKAVGL"
     gene            complement(5326..6015)
                     /gene="scpB"
                     /locus_tag="VFC01_08535"
     CDS             complement(5326..6015)
                     /gene="scpB"
                     /locus_tag="VFC01_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZN79718.1"
                     /translation="MRTEIPADLDLDIDIATAPELEDSELGAVLEALLLVVDTPVTVD
                     QLAAATEQPAYRVAAKLQLMAEELTTRESGIDLREAGGGWRMYTRARFAPYVERLLLD
                     GARSKLTRAALETLAVVAYRQPVTRARVSAVRGVNVDAVMRTLLARGLITEAGVDPDT
                     GAVTFATTELFLERLGLTSLADLPDIAPLLPDVDVIDDLSESLGEEPRFMKLSGAPAA
                     ERPMPFDVDQE"
     gene            complement(6032..6865)
                     /locus_tag="VFC01_08540"
     CDS             complement(6032..6865)
                     /locus_tag="VFC01_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HZN79719.1"
                     /translation="MNTEIQPEEQTRQSGFQVRLSNFEGPFDLLLQLIFAHRLDVTEV
                     ALHQVTDDFIAYTKAIGPQMELDETTAFLVVAATLLDLKAARLLPAGEVHDEEDLALL
                     EVRDLLFARLLQYRAFKHVAEMFAELEAAALRSYPRSVALEQRYEDLLPEVMIGVDAE
                     SFAQIAAAAFTPRPVPTVATEHLHQVMVSVPEQVGNLMALLETRGIGQWATFSDLVAD
                     CQAPIEIVGRFLALLELYRARAVAFEQSEALGVLQVSWTGERPTNDHLATADAEEVDD
                     D"
     gene            complement(6862..7722)
                     /locus_tag="VFC01_08545"
     CDS             complement(6862..7722)
                     /locus_tag="VFC01_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZN79720.1"
                     /translation="MTDEGGGAELGLTGRAPRTIPEPKPKTVHGPAKVIAMCNQKGGV
                     GKTTSTINLGASLSEFGRRVLLVDLDPQGALSAGLGVPHYELEHTIHNLLVEPRVSID
                     DVVIHTRVKHMDLVPSNIDLSAAEIQLVNEVGREQSLARALYPVLDRYDYILIDCQPS
                     LGLLTVNGLACSDGVIIPTECEFFSLRGLALLTDTVDKVHDRLNPKLSISGILITRYD
                     PRTVNSREVMARVLERFGDLVFDTVITRTVRFPETSVAGEPITTWAPKSGGAEAYRSL
                     AREVIDRFGA"
     gene            7862..>8331
                     /locus_tag="VFC01_08550"
     CDS             7862..>8331
                     /locus_tag="VFC01_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HZN79721.1"
                     /translation="MSLKQRVPFLRWSFWRLAIGARNISKTGQIGDGREAAAADYVVA
                     NARRGDIDDVIAKVDEFAYEKSFLINVGDEKGELLDAAVRRANPGLALELGTYCGYGS
                     LRIARAAPSAKVYSVELSAANAKVAQRIWAHAGVGDRVTCVVGAIGDGGRTLDAL"
BASE COUNT         1418 a         2998 c         2558 g         1357 t
ORIGIN      
        1 cggacgacgt cgctcttcgg cgccgaatca ccgtcatcat tggtctcgaa tacgctgcac
       61 ggacagccgc cgatttacac gaacgagaga ggccagcatg gcagcaccga gtcctggata
      121 ctccatcaca gtccgggttg aggcgccggc ttcgtcgagg gccaccggtg agctcacggc
      181 agcgatcggc gccgccggcg gcgcgctgac cgcgctggac gtggctgagt cgcgtcacga
      241 ctcgatcgtc gtcgacgtca cctgcgacgt cgcggacgcc gcgcacggcg acaccatcac
      301 cgaggcgatc gacgcgctgc ccggcttccg cgtccgcaag gtcagtgacc ggacgttctt
      361 gatgcacttg ggcggcaagc tcgaagtggt gccaaaggtg ccgctgcgcc accgcgatga
      421 cctctcccgg gcctacaccc cgggcgtggc tcggatctgc caggcgatcg ccgctaaccc
      481 ggaggacgcc cgccgcctga cgatcaagcg caacactgtg gcggtggtga ccgacgggtc
      541 ggccgtgctc ggtctcggaa acctgggccc agcggccgca ctgccggtga tggaaggcaa
      601 ggcagcactg ttcaagcggt tcgccgaggt cgatgcgtgg ccagtctgcc tggacacgca
      661 ggacactgat cagatcgtcg agatcgtctg tgcgctggcg ccggtgtacg gcgggatcaa
      721 cctcgaggac atcgcggcac ccaggtgttt cgagatcgaa cggcgcctgc gggagcggct
      781 ggacatcccg gtcttccacg acgaccagca cggcaccgcg atcgtggtgc tcgccgcgct
      841 catgaatgca gtgcgagtgg tccacaagaa actgcccgac ctccgcgtcg tcatctccgg
      901 cgtcggtgcc gcaggatcgg cgatcatccg actgctgcag gcccagggcg tccaggacat
      961 tgtcgccgcc ggtcgaaaag gcgtgctgtc gcgcgacgag gattacgccg atccgacccg
     1021 ccggtggatc gcggagaaca ccaatccccg cggcgtcacc ggctccctca agcaggcgct
     1081 cgccgacgcc gatgtcttca tcggagtaag cgcaccccgg ttattggacg gcgacgatat
     1141 cgcgacgatg gccgaccggg cgatcgtgtt cgccctctcc aatcccgacc ccgaggtgga
     1201 cccgctcgaa gcgcgtcaac acgccgcggt ggtggccacg gggcgcagtg actttcccaa
     1261 ccagatcaat aatgtgctcg cgttccccgg cttctttcgt ggcttgctcg atgccggggc
     1321 gcacgagatc aacgacgaca tgatgatcgc tgcggcgaag gcgatcgcgg aaatcgtcac
     1381 cccggaggag ctcaacccga gcttcatcgt gcccagcgtg ttcgacagca cggtggcacc
     1441 cgcagtcgcg gccgccgtca gcccagtcgc gagcgagcca gaccgggatt ccggtcttcg
     1501 gcagaggtga tctcggggca ggtcagagcg ttgcgacttg gtcgggctga caggatttga
     1561 acctgcgacc acttgacccc cagtcaagtg cgctaccaaa ctgcgccaca gcccgcggtg
     1621 ctgctgggat cgccagcagc aggtcgaaag cctaccgcag accggcgcca gagccctaaa
     1681 tggttaccaa ccgatacagc ccgatggcac ccaccatcac gatcgcggcc cgcagcgcgt
     1741 tcggcgacag ccgccgtccg tagtgcgcgc cgaggaaccc tccgatcagg gaacccacag
     1801 cgatcaatcc cgccgcctcc caactgatcc ggtcgaatgc gacgaccgtg tacgccaccg
     1861 cggccacgat gttcaccagc aacgacagca ggttcttcgc cgcgttcatc cgctgcatgt
     1921 cctccggcaa cagcgcactc atcgctgcga tcagcatgat gccctgcgcc gcggtgaagt
     1981 atccgccgta gatcccgatc gcgaacgtgc cgaacaccag cgccgccatc cgccgtccgc
     2041 tcacatgctc cgccgaccgg cccgactcct cagcacgccg ccgcgcccac tcctgaattc
     2101 gtggaccaac cacgaccagg cccagcgcca gcaccagcag gaccggcaca accttttcga
     2161 acaccttctc cggcaggtgc agcagcagcc acgaacccaa cagcgcgccc accaacgacg
     2221 ccgggatctg ccagcgcagg cgatgccatt gcccgcgcag ctcacgccga tatccccacg
     2281 tgccggacac gccgccggca accagaccga ccgcgttcga catcgtcgcc gtcaccggcg
     2341 gatagcccag cgccaccagc gtcggaaagg tgatcagggt gcccgagccg acgattgcgt
     2401 tgatcgcccc cgccccgacg cctgccaggg cgatcaggat catatgggaa acgggcattt
     2461 gcccaaccct atcgaggcgt tgcgctgggc ccaaagtcgc tcagcgagac ttcgctccgc
     2521 gcttctcccg aacccttacg ttgatccgga tcgggctgcc ctcgaatccg aacgtttcgc
     2581 gtaaccgccg ctcaagaaac cgccgatagc cggcctcgag aaaacctgtg gtgaacaaca
     2641 caaacgtcgg cggccgcgcg gtcgcctgcg tggcgaacag gattcggggc tgtttacccc
     2701 cgcgcaccgg cggcggcgtc gccgcgacga tctccttgaa gaacgtgttc agccgtcccg
     2761 tcggcaccct ggtgtcccag gacgccagcg acgtctccag cgcaggcacc agtttctgca
     2821 cggcccggcc ggtcttggcc gagatgttga cccgcggcgc ccattgcagt tgcgccagtt
     2881 cgcgatcgat ctcgcggtcc agcaagtaac gccggtcctc gtcgaccatg tcccacttgt
     2941 tgaacgccaa caccagcgcc cggcccgcct cgatcaccat cgacagcacc cgctgatcct
     3001 gctcggtcag cggttgcgac gcgtcgatca gcacgatggc cacttcggcc gcatcgatcg
     3061 cgccgtgcgt gcgtaccgac gcgtagaact catgcccgct ggcctgtccg acctttcgcc
     3121 gcaatcccgc ggtgtccacg aaacgccaga ccttgccgtc catttcgatc agcgagtcca
     3181 ccgggtcgac ggtcgtgccc gcgacgtcgt gcaccaccga ccgttcgtcg cccgcaaggc
     3241 gattcaacag cgagctcttg cccacattcg gcttgcccac caacgcaact cgacgagggc
     3301 cgcccccgcc cgacgccatt tcggagaccg cgggcagcgc atcgatcacc ttgtccagca
     3361 gatcggctac cccgcgaccg tgcatcgcgc tgatcgggtg cggctccccc agccccaacg
     3421 accacagctc cgccgcatcg gcctcgccac gctcgttgtc aaccttgttg gccgccaaga
     3481 agactggctt acccgaccgc tgcagcagcc gcgctgccgc ctcgtcggcc gtcgtcgccc
     3541 ccactgtcgc atcgaccacg aagacgatcg cgtccgcggt acgcatcgcc accgacgcct
     3601 gctcggccac caactgctgc aggcctttcg cgtccggctc ccatccgcca gtgtcctgca
     3661 ccacgaaccg ccgaccactc cacaacgcgt cgtaggacac ccggtcgcgc gtcacgcctg
     3721 gcacgtcctg caccaccgcc tcgcgacggc cgaggatccg gttcaccaac gtcgacttgc
     3781 cgacgttcgg gcgccccaca acagccacca caggtggcgg cgccgccgcc tcgatcgcct
     3841 cggcgacctc ttccgcgccg agctcccaat cgctttcgtc ggcccaggta ccctcactca
     3901 tcgcgacgca ccggcacgtt gttcgacgag ctccagcaga tgcacgacga cctcggtctc
     3961 ggtcatatcg ccggtgtcga ccaccaacgc gtcgtcggcc gcccgcaacg gggacacggc
     4021 ccgcgtcgaa tccagatggt ctcgccgctt gacgtcggcc agcacggcct cgtagtcgtc
     4081 gcccaacccg gacgcaatgt tctggtcgtt gcgtcgccgc gcgcgttcct cggccgacgc
     4141 ggtcaggaag atcttcacgt cggcgttggg aagtacgacg gtgccgatgt cgcggccctc
     4201 tacgacgacg tggcctgggc ccgcggcgag ttcgcgctgc aatcccacca accgtgcgcg
     4261 aacggccggc accgctgaga ctgctgacac cgctttggtc acctcgtcgc cgcggatctc
     4321 ggccgaaacg tcttctgcgt caaggtatgc ccggtcttca tccgggttgt agccgaccga
     4381 cagcgggaca tccgcggcgg ctgtctcgat ggcgggggtg tcggcaagat cgacgcctgc
     4441 gcgcaacact gccagcgtca cgatccggta catcgcaccg gtatccaagt accgcgcgcc
     4501 caacgatcgg gccaaacccc ttgataccga agactttccg gtgccggcgg ggccgtcgac
     4561 cgcgatcacc agtgaagcgc tcacaggccc accgccttgt acaactcccc tacctccttg
     4621 cggctcaaca cccggatgct gccgggtcgt tgatcaccga gcgacaccga gccgatgtcg
     4681 gtacgcacca actcctgcac cggatgcccg accgccgcaa gcagccgccg cacgatccgt
     4741 ttgcggccct cgtgcaacgt cacccgcacc agggttttgc ccggcacctt gtccaccagc
     4801 gcgaaatcgt cgacatgtgc tggcccgtca tccaattcga tcccgtcacg cagcttgcgg
     4861 cccagcccgc gcggcaccga gccgtcgacc gtcgccagat acgtcttggg cacctcgaac
     4921 gacggatgca tcaaccggtg cgccaactcg ccgtcgttgg tcagcagcat cagcccttcg
     4981 gtgtccgcgt cgagccgccc gacgtgaaac agtttcttgt tgccgcgcac tcgatgttcg
     5041 accagatcgc cgatgcaggg ccgaccgcgg tcgtcggaca tcgtcgaatg catgcccttg
     5101 ggcttgttga tcgccagatg caccagcgtg tcgtcgaccg tcactcgcgc accgtcgacg
     5161 cggattaccg acacgtccgg gttcacccgc gtgcccagct cggtgacgat ccggtcgtcg
     5221 acctcgaccc ggccgtcgag gatcatcttc tcggcgacgc ggcgcgaagc aattccggcc
     5281 tgcgacaaca ctttctgcag acgcacgccc tcggagtccg ccatctcact cctggtccac
     5341 gtcgaacggc atcggccgct cggccgctgg cgcgccgctg agtttcatga aacgtggctc
     5401 ctcgccgagg gattcgctca ggtcgtcgat gacgtcgacg tcgggaagca gcggtgcgat
     5461 gtcgggcaag tccgccagcg acgtcaagcc cagccgctcc aggaacagct cggtcgtcgc
     5521 gaacgtcacc gcaccggtgt ccgggtcaac accggcctct gtgatcagcc cgcgcgccag
     5581 cagcgtgcgc atcaccgcat ccacgttgac gccgcgcacc gcgctgactc gggcccgcgt
     5641 caccggctgc cggtacgcca ccaccgccag cgtctccagt gccgcgcgcg tcagcttgga
     5701 tcgtgccccg tcgagcagca gccgctcaac gtacggcgca aacctcgcgc gggtgtacat
     5761 ccgccagcct ccgccggcct cccgcaggtc gatgccgctc tcgcgggtcg tcagctcttc
     5821 ggccatcaac tgaagctttg ccgccacccg gtaggccggc tgctcggtcg ccgcggccag
     5881 ctggtcgacc gtcaccggcg tgtccaccac cagcagcagc gcctcgagca ccgcgccgag
     5941 ttcggagtcc tccagctccg gcgcggtcgc gatgtcgatg tccagatcga ggtccgccgg
     6001 gatttcggtg cgcatttcgg tcgagaattc gtcagtcatc gtctacttct tccgcgtcgg
     6061 cggtagccag gtgatcgttg gttggccgtt ctccggtcca cgaaacctgg agcacaccaa
     6121 gcgcttctga ttgctcgaat gctacagctc gcgctcggta cagctcgagc agcgccagga
     6181 accggccgac gatctcgatc ggcgcctggc agtcggcgac caggtcggag aatgtcgccc
     6241 attgtccgat gccgcgggtc tccaacaacg ccatcagatt gccgacctgc tcgggcaccg
     6301 acaccatcac ctggtgcagg tgctcggtgg ccacggtcgg caccgggcgc ggcgtgaatg
     6361 ccgcggcggc gatctgcgcg aagctctcgg cgtcgacgcc gatcatcacc tcgggcagca
     6421 ggtcctcata gcgctgctcc agggccaccg accgcggata actgcgcagc gctgcggcct
     6481 cgagctcggc gaacatctcc gcgacgtgtt tgaacgcccg gtactgcagc agccgcgcga
     6541 acagcaggtc gcgcacctcg agcagcgcga ggtcctcctc gtcgtgcacc tcaccggcgg
     6601 gcagtagccg cgccgccttg aggtccagca gcgtggccgc gacgacgaga aacgccgtgg
     6661 tctcgtcgag ttccatctga gggccgatcg ccttggtgta tgcgatgaaa tcgtcggtca
     6721 cctgatgcag agccacctcg gtgacatcga ggcggtgggc gaagatcagc tgcagcagca
     6781 gatcgaacgg cccctcgaaa ttgctcagcc ggacctggaa gccgctctgt ctggtctgct
     6841 cctccggctg tatttccgtg ttcacgcgcc gaaccggtcg atgacctcgc gcgccagcga
     6901 ccggtatgcc tcggccccac cggatttcgg tgcccacgtg gtgatcggct cgccagcgac
     6961 gctggtctcg gggaaacgca cagtgcgggt gatgacggtg tcgaacacca ggtcgccgaa
     7021 cctctccaga accctggcca tcacttcgcg agagttgacg gtgcggggat cgtaacgggt
     7081 gatcaggatg ccgctgatcg acagcttcgg gttcaaccgg tcgtgcacct tgtcgacggt
     7141 gtcggtcagc agcgcgaggc cgcgcagcga aaagaactcg cattcggtcg ggatgattac
     7201 cccgtcgcta caggccagcc cgttcacggt gagcagaccc agtgacggct gacagtcgat
     7261 caggatgtag tcgtagcggt ccagcactgg atacagcgcc cgggccagcg actgctcgcg
     7321 gcctacctca ttgaccaact ggatttcggc cgcggacagg tcgatattgc tcggcaccag
     7381 gtccatgtgc ttgacccggg tgtggatcac cacgtcatcg atcgacaccc gcggctcgac
     7441 cagcagattg tggatcgtgt gctcgagctc gtagtgcggc acccccagcc ccgccgacag
     7501 tgctccctgc ggatccaggt cgaccagcag cacgcggcgg ccgaactcgg aaaggctggc
     7561 gcctagattg atggtcgacg tggtcttccc gacgccgcct ttctggttac acatcgcgat
     7621 gaccttggcc gggccgtgca ccgtcttcgg cttgggctcg ggaatggtcc gcggcgcgcg
     7681 gcccgtcaga ccgagttcgg cgccgccccc ttcgtcggtc atgcccgacc gtcgctggtc
     7741 aggcacgcag agggcattcc gaacatccgg gcaagtttaa cgggaggtga ctgccagcat
     7801 cggcagaccc gcgggcaacg tggaacccaa tcgattagtc cgtccgaata ggctgacggc
     7861 catgagcctg aagcagcggg tgccgttcct gcgctggtcg ttttggcggc tggcgatcgg
     7921 ggcccgcaac atctccaaga ccggccagat cggcgacggc cgggaggcgg ccgcggccga
     7981 ctatgtggtg gccaacgcac gtcgcggcga tatcgacgac gtgatcgcca aggtggacga
     8041 gtttgcgtac gagaagtcgt tcttgatcaa cgtcggcgac gagaagggcg agttgctcga
     8101 cgcggctgtc cggcgagcca accccggcct ggctctggag ctgggcacct actgcggtta
     8161 cggctcgctg cggatcgccc gcgcagcacc gtcggccaag gtgtactccg tagagctgtc
     8221 ggccgccaat gccaaagtgg cgcagcgcat ttgggcacac gcaggcgtcg gcgaccgggt
     8281 cacctgcgtg gtcggcgcca tcggcgacgg cggccggaca ctcgacgcgc t
//
LOCUS       DASRJA010000192        13143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2735308,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000192 DASRJA010000000
VERSION     DASRJA010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13143
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2735308"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..2155)
                     /locus_tag="VFC01_08555"
     CDS             complement(95..2155)
                     /locus_tag="VFC01_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012833320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZN79722.1"
                     /translation="MGATFAYVMLDVAVIIVVARLFGRIARMFRQPAVIGEIVAGIAL
                     GPSLLGLLPGHVDKLLFPPDVLPYLKILAQLGLVLFMFIVGLELDVTLIRGRQRRAGT
                     ISLLSIVLPFALGALLALSLHPLHDVVDGKPVPLLGFVLFLGVAMSITAFPVLARILA
                     ERRMNRTSVGVLALSAAAVDDILAWTLLAFVYAVVKGDSPMSVVRIVALSAVFVTVMF
                     AVVRPLLARMLSWYERAGRVTPDMLAVVLVGVLLSALITERIGIHEIFGAFLFGAIMP
                     RQGAVEFRREILERLEQISVLLLLPIFFVIAGFGVNLRAFKDPGLWWQLLLILAVAIG
                     GKFVGAFVGARIQHMSFQQSAAIAVLMNTRGLTELVILLIGKQVGVLDTAMFTMMVVM
                     ALVTTVMTEPLLRIVYPAKAVQRDIDAATKAALGSEQTYRVLVVVDAVPNAATERMLR
                     VADAALADRRPGEIMLSRFLETPEDAPPLELGSGPLPDLAGMAEAVESLEQFATQTTT
                     TAATLRVLCRFGSASGQDVIRQVETSGAEVVVVSDVWAHKHPQAYDALDAVTVLVVPA
                     ELPTVWLAATNEAAVGVADDAVYVRDDGAANGARAVLVAVRAATASGRSLIALVSETD
                     GRARRRLQAALEPLRSTSTRVSVVSVDGGGVVAGADVSAVARVVDRVGGSQVNLRDAV
                     ADVV"
     gene            2205..3017
                     /locus_tag="VFC01_08560"
     CDS             2205..3017
                     /locus_tag="VFC01_08560"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005201019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN79723.1"
                     /translation="MHVDAESIPAAIADYRILGLLGEGNNGRFYLAEAPERLGLSTDR
                     VALKVFTAPAGKDAYRRGVRELRAFAAVGSPRLVRIYDVVLEDNFLYATEYFPLGSLA
                     SPARTLTRTEILCALEDAARAVHELHEHGLVHADIKPANVMVADDGGRLSDLGLARVL
                     TSEGSVTSFAPATSAEFVDPDLLQGEMPSRATDVWSLGATINRALSGEGLYGELPIDQ
                     PMLAIRAAVSGKTRVSAKLDRAESGLVHHCIAPVASRLATAAEVADGIAALR"
     gene            complement(3044..6199)
                     /gene="ileS"
                     /locus_tag="VFC01_08565"
     CDS             complement(3044..6199)
                     /gene="ileS"
                     /locus_tag="VFC01_08565"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZN79724.1"
                     /translation="MTAYPKPAAGAPKFPALELDVLDYWEKDDTFRASIERRDGAPEY
                     VFYDGPPFANGLPHYGHLLTGYVKDIVPRYRTMRGYKVERRFGWDTHGLPAELEVQRQ
                     LGITDKAQIEELGIEKFNDACRASVLKYTNEWQAYVTRQARWVDFENDYKTLDPTFME
                     SVIWAFKQLWDKGLAYEGNRVLPYCWNDETPLSSHELRMDDDVYQSRQDPAITVGFRV
                     DGGDDKELAGAFLLVWTTTPWTLPSNQAVAVNPEVTYVLVQGPDDRRYVLAQARLSAY
                     ARELGEEPEVLATYTGQDLLETHYLPPFPYFMDVPNSFRVLPADFVSTEDGTGIVHMS
                     PAYGEDDMATTQAAGIPAVTPVDAKGRFDETVPDYAGQHVFDANPQIIRDLKNQGRSA
                     AVNGAVLLRHETYEHSYPHCWRCRNPLIYRAVSSWFIKVADFRDRMVELNKDITWYPE
                     HVKDGQFGKWLSGARDWSISRNRYWGTPIPVWKSDDPAYPRIDVYGGLDELERDFGVR
                     PADLHRPYIDELTRPNPDDPTGKSTMRRIEDVFDVWFDSGSMPYAQVHYPFENQKWFD
                     GVSGEDGQTGVDAHFPGDFIVEYIGQTRGWFYTLHILATALFDKPAFKTCVAHGIVLG
                     NDGQKMSKSLRNYPDVTEVFDRDGSDAMRWFLMASPILRGGNLIVTEQSIREGVRQVL
                     LPLWNAYTFLALYAPKKGTWHTDSSHVLDRYILAKLAELRDDLTESLDVCDIYGACDH
                     LRQFTEALTNWYVRRSRQRFWEEDPDAIDTLHTVLEVTARLAAPLLPLITEVIWRGVT
                     GERSVHLTDWPEAGVVPADPELVTAMDQVREVTSAASSLRKAKQLRVRLPLPKLTVAV
                     ENPQRLEAFKDLIADELNVKTVELTDDIDTYGRFELTVNAKVAGPRLGKDVQAAIKAV
                     KAGDAVVNDDGTLTAGPALLQPEEYSSRLVAADPEYTVALPDGAGLVVLDGTVTPELE
                     AEGWAKDRIRELQELRKSTGLDVSDRIRVTIAVPEDKAQWAQTHSELIAREILATSFE
                     FGEPVDGTEIGDGVRVAIAKA"
     gene            complement(6434..7012)
                     /locus_tag="VFC01_08570"
     CDS             complement(6434..7012)
                     /locus_tag="VFC01_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN79725.1"
                     /translation="MRAAGRATRDRILAAAKDEFTEYGFAGARLNRIASDARASKERL
                     YSYFASKEQLFEAVVAQWIEDAPYRVPLTAEDVPGYVEGLFDNFVADPRGARLQRWIE
                     LEAPSGLSDDHLLRRLFRAKLAEVRRGQQAGLIDPSWHPMDLLTLLTEIVQSMAAGAS
                     VVSSIVGEARGRDTLAERRAAAAEAARRLVAY"
     gene            complement(7018..8085)
                     /locus_tag="VFC01_08575"
     CDS             complement(7018..8085)
                     /locus_tag="VFC01_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZN79726.1"
                     /translation="MKVTAAVSRSPDSPFTLESVDLDEPRPDEVLVRISATGLCHTDL
                     SSKSLMPGPAVLGHEGAGVVEAVGARVSGITPGDHVLLSYRSCGECAQCRGGHRSYCS
                     RGALLNYSGGRADGTATLSQNGTRLFGSFFGQSSFAQYALAAADNVVVVDPSVDLTVA
                     APLGCGFQTGAGAVLNVLSPEPNSRLAVFGAGGVGLAAVMAAKAVGVEAVTVVDPVAS
                     RRAKAVELGASQVVDPSSEDVAAAVQGATHALDTTGLGDVVAAALSALESRGILAVVG
                     LGARHVTIDITDLLMNGKMIRGCIEGDANPCEFIPELLTLHAQGKFPMDAIVRRYDAR
                     DIETAVADSLSGEAIKPVLVW"
     gene            complement(8085..8549)
                     /locus_tag="VFC01_08580"
                     /pseudo
     CDS             complement(8085..8549)
                     /locus_tag="VFC01_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733878.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4334 domain-containing protein"
     gene            8646..9083
                     /locus_tag="VFC01_08585"
     CDS             8646..9083
                     /locus_tag="VFC01_08585"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN79727.1"
                     /translation="MGESLTEEQQQRRRQAVVELMPSTPYMSWLGIVFDRYEPDEVTI
                     RLPFREELTNDGIYYHGGVIASVLDTAGAAAAWSNHDFEKGTRASTISMTVNYVGAAK
                     RSDLICHARTVRRRKELTFTEITATDADGNVVAHAVQTYRLGG"
     gene            complement(9065..9634)
                     /locus_tag="VFC01_08590"
     CDS             complement(9065..9634)
                     /locus_tag="VFC01_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN79728.1"
                     /translation="MTEARVPRLPIEEAKAAADQAGVPDYMAELSIFQVLLNHPALAR
                     AINDLLATMLWHGKLDRRLRELVIMRIGWLTACDYEWTQHWRVACGLGVLADDLLGVR
                     DWSTHHGFGAAERAVLAATDDVLRDGAVSAQTWAACERELDADSAVLIELVTAIGAWR
                     MIASILLSLGVPLEEGVDSWPPDGQPPSR"
     gene            complement(9631..10734)
                     /locus_tag="VFC01_08595"
     CDS             complement(9631..10734)
                     /locus_tag="VFC01_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN79729.1"
                     /translation="MPDSASARALVLERPRHLVIHDIPLPALGDDDALVRVEACGLCG
                     TDHEQYTGELAGGFAFVPGHETIGIIETIGPRAAQRWQVAEGDRIAVEVLQSCRACDA
                     CLTGEYTRCKRHGVADMYGFIDVDRAPGLWGGYAEYQYLAPDSMVLSLPDNLDPVLAT
                     LFNPLGAGIRWGATLPGTGDGDVVAVLGPGVRGLCVAAAAREAGAGFVMVTGFGPRDA
                     ERLALASDFGADLAIDVAVEDPVAALRHAAGRLADVVVDVTAKAPAAFAQAIKLVRTA
                     GTVVVAGTRGWGVGAPGFFPDMVVMKEIRILGALGVDTTAYRAALDLLASGRYPFADL
                     PRRCVGLDGAADLLATMAGDRDDIPPVHGVVTP"
     gene            10766..10981
                     /locus_tag="VFC01_08600"
     CDS             10766..10981
                     /locus_tag="VFC01_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HZN79730.1"
                     /translation="MSDHTYRIIEIVGSSPDSIDAAIRNGISRASETMRGLDWFEVES
                     VRGHLQDGAIGHFQVTLKVGFRLEDAR"
     gene            complement(10984..11628)
                     /locus_tag="VFC01_08605"
     CDS             complement(10984..11628)
                     /locus_tag="VFC01_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79731.1"
                     /translation="MVAPYLADQSSDLTYPADNLAMPTLRVVDHDIASGTSPVLHKIA
                     AAPSEEVTAEVDGATEWLGVAADEILLAALGRAVARTIGDGVVAVDVTGERRWLLHAI
                     PLLCATAQQLGPTEMLGNVHRSLGAAPAHGTSLPSEIFLNYIGTLPDGTSPMYETPPG
                     LGYALELRVYRTDGLLHLDWWYDTTRFDRYTVEELTEQFPLALFEMTSDAAAPV"
     gene            11764..12129
                     /locus_tag="VFC01_08610"
     CDS             11764..12129
                     /locus_tag="VFC01_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZN79732.1"
                     /translation="MPRTDENGRQLKALLDYLLDGEIDAKDIYDALGTSSSTYYRRIK
                     EDDYPNAEELRRVADRFALSYPDLQIQFGLMSRQEVWSYIESARFTMTAVQEAVQTKT
                     TTRPPKLSELKPRADAPPL"
     gene            12181..12915
                     /locus_tag="VFC01_08615"
     CDS             12181..12915
                     /locus_tag="VFC01_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79733.1"
                     /translation="MALATLIAITLGCIGWSLWIRRVTWSCRWEVAATLNIALQGLAV
                     ILMSPLASETLGHWLHALTGMWNLEDYIGHDAYIVAASAVVYNALGRLADDHAMQTSF
                     KQYVERPATLCIPLLLVTFSIGNGASVYRSDFFEVPTDFWLNMYWLLLCGMLIYLLTY
                     GMRALMILRQDPRSRKIANIYLVASASGIMACTVRIITAYVPALQTVEGGTLVWIFAC
                     GCGAGFALTSAQSWRIKTKWFTRASR"
     gene            complement(12916..>13143)
                     /locus_tag="VFC01_08620"
     CDS             complement(12916..>13143)
                     /locus_tag="VFC01_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HZN79734.1"
                     /translation="GHAGLAGSVGWADPSTGLSFGFVHNRLLTRMLLDQATFAGLHAL
                     IRRDSAQARKRGYETMPAFGAPYDVPRPVAG"
BASE COUNT         2417 a         4420 c         4065 g         2241 t
ORIGIN      
        1 cgcgaatcct cggtgtcgct catcgccgac tcctcaggtg gttagccagc tctaacgcta
       61 aatcctgccc aggcccaccg cagcaaaacc gcactcacac aacatcggcc accgcatcac
      121 gcaaattgac ctgcgaacca cccacccgat caacaacgcg ggccactgcc gaaacatcgg
      181 cgcccgcaac cacgccaccc ccatcgacag aaaccacact gacacgcgtc gacgtggaac
      241 gcagcggctc gagcgccgcc tgtaggcgcc gacgcgctcg accgtccgtt tcagacacca
      301 aggcaatcag cgaacgtccc gaagccgtcg ccgcccgaac cgcgaccaac accgcacgag
      361 cgccattggc cgctccgtcg tcacgcacat agaccgcgtc gtccgccaca cccactgccg
      421 cctcattggt ggccgccagc catacggtcg gcagctcggc cggaacaacc agtacggtta
      481 ccgcgtcgag ggcgtcgtat gcctgcggat gcttgtgcgc ccaaacgtcg ctcaccacaa
      541 cgacttccgc cccggacgtt tccacctgac gtatcacgtc ctgaccggac gcggaaccga
      601 accgacacaa aacccgcagc gtggcagcgg tcgtcgtggt ctgcgtcgcg aactgctcca
      661 gtgattccac tgcctcagcc atacccgcga gatctggcag cgggccggat ccgagctcga
      721 gaggtggggc gtcctcgggc gtttccagaa atcgtgacag catgatctca ccaggtctgc
      781 ggtctgcgag tgccgcatcc gccacccgta gcatgcgctc cgtcgccgcg ttggggaccg
      841 cgtcgacaac gacgagcacg cggtaggttt gctcggatcc aagtgcggcc ttcgtcgcgg
      901 cgtcgatgtc gcgctgcacc gccttggccg ggtagacgat cctcaacagt ggttcagtca
      961 tcaccgttgt gaccagcgcc atcacgacca tcatcgtgaa catcgcggtg tcaaggacgc
     1021 cgacctgttt gccgatcagc aggatcacca gctcggtcag gccacgggtg ttcatcagca
     1081 ccgcaatggc ggcgctttgc tggaaagaca tgtgctggat gcgcgcaccg acgaaagccc
     1141 cgacgaactt gccgccgatg gccaccgcca gaatcagcag gagctgccac cacagccccg
     1201 gatccttgaa cgcgcgcaga tttactccga agccggcgat cacaaagaag atcggcaaga
     1261 gcagaagcac actgatctgt tcaagtcgtt ccaagatctc gcgcctgaat tccacagcac
     1321 cctgccgcgg catgatcgca ccgaaaagga acgcgccgaa aatctcgtgg ataccgattc
     1381 tttcggtgat caacgcggac agcaatacac cgacgagaac aacggccagc atgtcgggag
     1441 tgacccgacc tgcccgctcg taccaggaaa gcatccgggc cagcagcggc cgcacgaccg
     1501 cgaacatcac cgtgacaaac accgcggaca acgcgacgat gcgcaccacc gacataggac
     1561 tgtcgccctt tacgaccgcg tagacgaagg ccagcaaggt ccacgccaag atgtcgtcca
     1621 ccgctgcggc cgaaagcgcc agcactccaa cgcttgtgcg gttcattctg cgttcggcaa
     1681 ggatccgtgc caacacggga aatgcggtga tggacatcgc tacacccaga aagagcacaa
     1741 accccaacaa cggaactggc ttgccgtcga ccacgtcgtg gagcggatgc aagctcaatg
     1801 ccaacaacgc acccagcgcg aaaggcagaa cgatggacag caacgagatc gtgccggcgc
     1861 gacgctggcg gccgcgaatc agtgtgacgt cgagttcgag gcccacgatg aacatgaaca
     1921 gcaccagccc gagctgcgcg aggatcttca ggtaaggcag cacgtcggga ggaaaaagga
     1981 gcttgtcaac gtgcccgggc agaaggccga gcaggctcgg gccgagcgcg atccccgcga
     2041 cgatttcgcc tatcacggcc ggttgccgaa acatccgtgc gattcgcccg aagagtctcg
     2101 cgaccacgat gatcacagcg acatcgagca tcacataggc gaatgtggca cccatggcaa
     2161 acagtgcacc ataattagcc tgctacggag caaaggaccg tgaaatgcac gtcgacgcag
     2221 aatcgatacc cgccgcgatc gccgactacc ggatcctcgg cctgctcggt gagggcaaca
     2281 acggccggtt ttacctggct gaagctcccg agcgtcttgg tttgtcgacc gatcgagtgg
     2341 cgctgaaggt tttcaccgct cctgccggca aagatgcata tcgacggggc gttcgtgagc
     2401 tgcgggcgtt cgccgcggtg gggtcgccca ggctcgtgcg catctatgac gttgtcctgg
     2461 aggacaactt cttgtacgca acggagtact ttccgctcgg ctcgctcgca tcacccgcgc
     2521 gaaccttgac gcgcaccgaa attctgtgcg cgctcgaaga cgcggcacgc gccgttcatg
     2581 agttgcacga gcatggtctg gttcacgctg acatcaaacc cgcgaacgtg atggtcgcag
     2641 acgacggcgg tcggctgtcc gacctggggc ttgcccgggt gctgacttca gagggcagcg
     2701 tgacaagttt cgccccggca acctcggcgg agttcgtcga ccctgacctc ttgcaaggcg
     2761 aaatgccctc acgtgcaacc gatgtctggt cgctcggcgc gacgatcaac cgggcactct
     2821 ccggcgaggg gctgtatggc gaacttccga tagatcagcc gatgctagcc attcgtgccg
     2881 cggtatccgg gaagacacgg gtgtcggcga agctggatcg agctgagtcc ggattggttc
     2941 accattgcat tgctccggtg gcaagccgtc ttgcgaccgc ggctgaggtg gccgacggca
     3001 tcgccgctct gcgttaactc gccatcttga cttcggatta cgactaagcc ttcgcgatcg
     3061 ccacccggac gccgtcgccg atctcggtgc cgtcgaccgg ctcgccgaat tcgaaactgg
     3121 tggccaggat ctcgcgggcg atcagctcgc tgtgcgtctg cgcccactgc gccttgtcct
     3181 cgggcaccgc gatggtcacc cggatccggt cggacacatc gaggccggtg gacttgcgca
     3241 gttcctgcag ttcgcggatg cggtccttgg cccagccctc ggcctccagc tcgggggtaa
     3301 cggtgccgtc gagcacgacc agtcccgctc cgtcgggcag cgcgacggtg tattcggggt
     3361 cggcggccac cagccgcgag ctgtactcct cgggttgcag cagggcaggt cctgcggtca
     3421 gcgtgccgtc gtcattgacg accgcgtccc cggccttcac ggccttgatc gccgcctgca
     3481 cgtccttacc cagccgcggc ccggcgacct tggcgttgac ggtcagctcg aaccggccat
     3541 aggtgtcgat atcgtcggtg agctcgaccg ttttcacgtt gagctcgtcg gcgatcaaat
     3601 ccttgaacgc ctcgagccgt tgcggattct ccaccgcgac agtcagtttt ggcagcggca
     3661 gccgcacccg cagctgcttc gcctttcgca gcgacgacgc cgccgacgtc acctcacgca
     3721 cctgatccat cgccgtgacc agttccggat ccgccggcac cacgccggct tccggccagt
     3781 ccgtgagatg caccgaccgc tcgccggtta caccgcgcca gatcacctcc gtgatcagcg
     3841 gcagcagtgg cgccgccagc cgggccgtca cttccagcac cgtgtgcagc gtatcgatgg
     3901 cgtcggggtc ctcctcccag aaccgctgac gcgagcgccg cacataccag ttcgtcaacg
     3961 cttccgtgaa ctgacgcagg tggtcgcagg caccgtagat gtcgcagacg tccaacgact
     4021 cggtcaagtc gtcgcgcagc tcggccagct tggccaggat gtagcgatcg agcacgtgcg
     4081 acgaatcggt gtgccaggtg cccttcttcg gcgcatacag cgcgaggaac gtgtaggcat
     4141 tccacaacgg caacagcacc tgccggacgc cttcgcggat gctctgttcg gtgacgatca
     4201 ggttgccgcc ccgcaagatc ggcgaggcca tcaggaacca gcgcatggcg tcggagccgt
     4261 cgcggtcgaa cacctcggtc acgtccggat agttgcgcag cgacttgctc atcttctgcc
     4321 cgtcgttgcc gagcacaatg ccgtgcgcca cacaggtttt gaacgcgggc ttgtcgaaca
     4381 gcgcagtggc caggatgtgc agggtgtaga accagccgcg agtctgaccg atgtactcga
     4441 cgatgaagtc gccgggaaag tgcgcgtcga cgccggtttg cccgtcctcg ccactcacgc
     4501 cgtcgaacca cttctggttc tcgaacgggt agtgcacctg tgcataaggc atcgaacccg
     4561 agtcgaacca cacgtcgaag acgtcctcga tgcgacgcat cgtggactta ccggtcgggt
     4621 cgtcggggtt gggccgcgtc agctcgtcga tgtacggccg gtgcagatcc gcgggccgga
     4681 cgccgaagtc gcgctccagc tcgtcgaggc cgccgtacac gtcgatgcgc ggatacgccg
     4741 gatcgtcaga cttccatacc ggaatcggtg tgccccagta ccggtttcgc gagatcgacc
     4801 aatcccgggc gccggacagc cacttgccga actggccgtc cttgacgtgt tcgggatacc
     4861 aggtgatgtc cttgttcagt tcgaccatgc ggtcgcggaa gtcggcgacc ttgatgaacc
     4921 acgacgatac ggcccggtag atcaacgggt ttcggcaccg ccagcaatgc gggtaggagt
     4981 gctcgtaggt ctcgtggcgc agcaacaccg caccgttgac ggccgccgag cggccctggt
     5041 tcttcaggtc tcgaatgatt tgcggattcg cgtcgaacac atgctgaccg gcatagtccg
     5101 gcacggtctc gtcgaagcga cccttggcgt ccaccggcgt gaccgcgggg atgccggccg
     5161 cctgcgtcgt cgccatgtcg tcctcgccgt acgccggcga catatgcacg atgccggtgc
     5221 cgtcttcggt gctgacgaag tcggcgggca gcacccgaaa cgagttcggc acgtccatga
     5281 agtacgggaa cggcggcagg tagtgcgtct ccagcaggtc ctggcctgtg taggtggcca
     5341 ggacttcggg ctcctcgccg agctcgcggg cgtacgcgga cagccgcgcc tgcgccagca
     5401 cgtagcgcct gtcgtcgggg ccctgaacca ggacgtaggt gacctccggg ttgaccgcga
     5461 cggcttggtt cgacggcagg gtccacggcg tcgtcgtcca gactaagagg aaagcaccgg
     5521 ctagttcctt gtcgtcgccg ccgtcaacgc ggaagccgac agtgatcgcc gggtcctggc
     5581 ggctttggta gacgtcgtcg tccatccgca gttcgtggct ggacagcggg gtttcgtcgt
     5641 tccagcagta cggcagcacc cgattgcctt cgtaggcgag ccctttgtcc cacagctgct
     5701 tgaacgccca gatcaccgat tccatgaacg tcggatccag tgtcttgtag tcgttttcga
     5761 agtccaccca gcgggcctgc cgggtcacgt aggcctgcca ttcgttggtg tacttgagca
     5821 ccgacgcgcg gcacgcatcg ttgaacttct cgatgcccag ctcttcgatc tgcgccttgt
     5881 cggtgatgcc gagctggcgc tggacctcga gttcggcagg caggccgtgg gtgtcccagc
     5941 cgaaccggcg ctccaccttg tagccgcgca tggtgcggta ccgcggaacg atgtccttga
     6001 catagccggt cagcagatgc ccgtagtgcg gcaggccgtt ggcgaagggc gggccgtcgt
     6061 agaacacgta ttccggtgcg ccgtcgcgac gctcgatgct ggcgcggaac gtgtcgtcct
     6121 tttcccagta gtcgaggacg tcgagttcga gcgccgggaa cttcggagct ccggcggccg
     6181 gcttgggata ggcggtcact gtggtgcgtt gtctcctgtg ccgaaacctc aggcacgggg
     6241 acgacgtcgc gccggtgcgc gagcgccgcg gtaccacccc gcttgcacac acaaacgtgt
     6301 gcgccgctca actgcgggcg atgacgggcc cacccgttcg gttctactgg gctgcatcag
     6361 ccgttcttcc gaaggctccc cggtgatggc cggatcggtg ccgttcactg atgagtctaa
     6421 tggccgtcgc aaatcagtag gcgaccaggc ggcgggccgc ttccgccgcg gcggctcgcc
     6481 gctcggccag cgtgtccctg ccccgcgctt cgcccacgat gctgctgacg acggaagcac
     6541 cggcggccat cgactgcacg atctcggtca gcagcgtcag caggtccatg gggtgccagc
     6601 tcgggtcgat caggccggcc tgctggcccc tgcgcacctc cgcgagcttt gcccgaaaca
     6661 gccgtcgaag caggtgatcg tcggacaacc ccgagggggc ctccagttcg atccagcgct
     6721 gcagccgggc cccgcgcgga tcggcgacga agttgtcgaa cagtccctcg acgtagcccg
     6781 ggacgtcttc ggcggtcaac ggcacccgat agggcgcgtc ctcgatccat tgcgcgacga
     6841 cggcctcgaa cagctgctcc ttactcgcga agtagctgta cagccgttcc ttactcgcgc
     6901 gcgcatcaga cgcgatccgg ttcagccgcg cgcctgcgaa accgtattcg gtgaattcgt
     6961 ccttggccgc ggccaggatg cgatctcgtg ttgcccgtcc tgctgctcgc atcgcagtca
     7021 ccagaccagc actggcttga ttgcctcgcc ggacagggaa tcggcgacgg ccgtttcgat
     7081 gtcgcgtgcg tcgtagcgcc gcacgatcgc gtccatcgga aacttgccct gagcgtggag
     7141 ggtgagaagt tccgggatga actcacacgg atttgcgtca ccctcgatgc acccgcgaat
     7201 catcttgccg ttcatcaata gatcggtgat atcgattgtc acgtgccgcg cacctagacc
     7261 gaccaccgcc aatattcctc gtgattcaag cgcgctcagt gccgctgcga cgacgtctcc
     7321 aagcccggtc gtatcgagcg cgtgcgtggc tccctggacc gccgccgcga cgtcctcgga
     7381 cgacgggtcc acaacttgac tcgcaccgag ttcgacggcc ttggcccgtc gcgacgcaac
     7441 cggatcgacc acagtgacgg cttcgacgcc gaccgccttg gctgccatca ccgcggccag
     7501 gccgacacca cctgcaccga acaccgccag ccgcgaattc ggctcggggc tgagaacatt
     7561 caggaccgcc ccggcgcccg tctggaaccc gcacccgagc ggagccgcga cggtgagatc
     7621 aacagatggg tcaacgacga cgacattgtc cgcggcggca agcgcgtatt gtgcgaagct
     7681 cgattggccg aagaacgacc cgaacaggcg agtcccgttc tgggacaagg tcgccgtgcc
     7741 gtccgcacgg ccacccgagt agttcagcag cgcgcctcgc gaacagtagg accgatgacc
     7801 gcctctgcac tgtgcgcact caccgcagct gcgataactc aacagaacat ggtcgcccgg
     7861 tgtaatcccg gacacccgcg cacccaccgc ctcgaccacg ccggcgccct cgtgcccgag
     7921 gacagcggga cctggcatca gggacttgga gctcaagtcg gtgtggcaca gcccggtcgc
     7981 ggatatccgg acaagcacct cgtccggtcg cggctcgtcg aggtcgaccg attcgagcgt
     8041 gaacggcgaa tccggcgacc gactcaccgc ggcagtgacc ttcatcaggc tcgttcgagc
     8101 aggaagtaca aatagttgcc gctggctcgc tgccgcttgc cgttcatgat gcccatcagc
     8161 gtgtcgtcgt cgaccttctt gaagtggtcg aagacgggct ggccgtcgta caccatcgtg
     8221 gccgtgacgt cgccacggaa ttcgatgtcc cacaggctgg cgccgccgcg gctcaactcg
     8281 ttgttggaga acaggtttcc gtcatcgtct tagcagacaa tcggctcgac gtcgtcgacg
     8341 gagtggaaga tctttccgaa ccagcgcgcg gccttcaact gcccgttcat cttgtgcccg
     8401 gtgtggaatt cgtcgcccct ccaggtgccg acgatgtcag cggatcggac ggtgtcgagc
     8461 gccgtccaga cctcgtcgag atcggcgggg ctcacgtcgc gctcgccgcg cagttcggcg
     8521 aagcgcgtcc gagccttctc tatgtccatg gccgtcctcc cattgccaaa ccatctggtt
     8581 cggaaacgag atgctactcc cgaaccagtt ggttcggcaa caccgcgtca gtaacgtgct
     8641 cgcgtatggg cgagtcactg accgaggagc agcagcaacg acggaggcag gcagtggtcg
     8701 agctgatgcc gtccacgcct tacatgagct ggctgggcat cgtgttcgac cgctacgaac
     8761 ccgacgaggt caccattcgg ctgcccttcc gcgaggagct caccaacgac ggcatctact
     8821 accacggcgg cgtcatcgcc tcggtcctcg acacggcagg cgcagcggcg gcgtggtcga
     8881 atcacgactt cgagaagggg acgcgcgcct ccaccatttc gatgacggtc aactatgtcg
     8941 gtgcagccaa gcggtccgac ctgatctgcc acgcccgcac cgtgcgcagg cgcaaggaac
     9001 tcacgttcac cgagatcacc gcgaccgacg ccgacggcaa tgtcgtcgcg catgcggtgc
     9061 agacttaccg actcggcggc tgaccatccg gcggccagct gtcgacaccc tcctccaacg
     9121 gcaccccgag gctgagcaga atcgacgcga tcatccgcca cgccccgatc gcggtgacca
     9181 actcgatcaa cacggccgaa tccgcgtcga gttcgcgttc gcaggccgcc cacgtttgcg
     9241 cgctcaccgc accgtcgcgc aggacgtcgt ccgtcgccgc gagtacggca cgctccgccg
     9301 ctccgaatcc gtgatgggtt gaccagtccc gcacaccaag gaggtcatcg gcgagcacgc
     9361 caagcccgca ggcgacccgc cagtgctgcg tccattcgta gtcacaggcg gtcagccagc
     9421 cgatccgcat gatcaccaac tcgcgcagac gccggtcgag cttgccgtgc cacaacatcg
     9481 tcgctaacag atcgttgatg gcccgcgcga gcgcggggtg attcagcagc acctggaaga
     9541 tggacaattc agccatgtag tcggggacgc ccgcttggtc ggcggcggcc ttggcctcct
     9601 cgatcggtag ccgcgggaca cgagcctcgg tcacggtgtc accaccccgt gcacgggcgg
     9661 gatgtcgtca cggtcgccgg ccatcgtggc gagcagatca gccgctccgt cgagccccac
     9721 gcaccgccgc gggaggtccg cgaacgggta tcgcccggac gccagcagat cgagcgccgc
     9781 acggtatgcc gtcgtgtcga caccgagcgc gccgaggatg cggatctcct tcatcaccac
     9841 catgtccgga aagaacccgg gcgcgccgac cccccaaccc cgcgtcccgg cgacgacgac
     9901 agtgccggcg gtgcgaacta gcttgatcgc ctgcgcgaac gcggcgggcg ccttggcggt
     9961 gacgtcgacg acgacatcgg ccagccggcc tgcggcgtgg cgcagcgcgg ccacgggatc
    10021 ttcgaccgcg acgtcgatgg cgaggtcggc gccgaagtcc gatgcgagtg caagccgttc
    10081 ggcgtcccgc gggccaaatc cggtcaccat cacaaacccc gcacctgctt ccctcgctgc
    10141 ggcggcgaca caaagcccgc gcacgcccgg cccgagtacg gcgacgacat cgccgtcacc
    10201 ggtgcccggc aacgttgcac cccaacggat tccggccccc aacgggttga ataacgtcgc
    10261 gagaaccgga tcgagattgt ccggcaaaga caggaccatc gaatccggtg ccaggtactg
    10321 atattcggca taaccgcccc acaagccggg tgcgcggtcg acatcgatga acccgtacat
    10381 gtcggcgacc ccgtgccgct tgcagcgggt gtactccccc gtcagacagg catcgcaggc
    10441 gcggcaggat tgaagcactt cgaccgcgat gcgatcgcct tcggctacct gccatcgctg
    10501 tgcagccctc ggtccgatcg tttcgatgat cccgatcgtc tcgtggccgg gaacgaacgc
    10561 gaatccgccc gcgagctcgc cggtgtactg ctcgtggtcg gttccgcaca gtccgcacgc
    10621 ctccacgcga accagcgcgt cgtcgtcgcc gagtgccggc agtgggatgt cgtggatcac
    10681 gaggtgacga ggccgttcca acaccaacgc acgcgcgctc gcggagtcgg gcatagccgc
    10741 accctaaacc gcgtagcgtc gcgtcatgag cgatcacacg taccgaatca ttgagatcgt
    10801 cgggagctcg cccgacagca tcgacgcagc gatccgcaac ggcatatctc gggcatcgga
    10861 gacgatgcgc ggcctcgact ggttcgaggt ggagtcagtc cgggggcacc ttcaggacgg
    10921 cgccatcggg catttccagg tcacgctgaa agtggggttc cgcctggaag atgcccgatg
    10981 acgtcatacc ggagcggcgg cgtctgaggt catttcgaag agcgcaagcg ggaactgctc
    11041 cgtgagctct tccaccgtgt agcggtcgaa gcgggtcgtg tcgtaccacc aatcgaggtg
    11101 cagcagtccg tcggtgcggt acacccgcag ctcaagggcg taccccaagc cgggcggcgt
    11161 ctcgtacatc ggtgaggtgc cgtcaggcag cgtgccgatg tagttgagga agatctccga
    11221 tggcagggac gtgccatgcg ccggcgccgc accgagcgag cggtgcacat tgcccagcat
    11281 ctcggtcggg ccaagttgct gtgcggtcgc gcagagcagc gggatcgcgt gcagcagcca
    11341 acggcgctcg ccggtaacat cgacggcaac cacaccatca ccgatggttc gcgcgaccgc
    11401 ccgcccgagc gcggcaagca agatctcgtc ggccgcaacc ccgagccatt cggttgctcc
    11461 gtcaacttcc gcggtcacct cttcggacgg ggccgcggcg attttgtgca ggaccggcga
    11521 ggtcccgctg gcaatgtcgt ggtcgacgac acgcaacgtg ggcatggcca ggttgtcggc
    11581 cggatacgtc aaatcgctgg actgatcagc taaatacgga gccaccatgc agctaaccct
    11641 acacctcatt cccagaaatg ggagactcct cccaaaactg tgacgccagt gtcaataatg
    11701 gctaattcat ccgatttgcg aagcgtgctg cccaaagccg ggatcaggag ctatcgtagg
    11761 tggatgccac ggaccgacga gaacggcaga cagctcaagg cattgctcga ctacctcctc
    11821 gacggagaga tcgacgccaa ggacatctac gatgcgctcg gtacctccag cagtacctat
    11881 taccggcgca tcaaagagga cgactatccc aacgccgagg agcttcggcg agtagccgac
    11941 aggttcgcgc tcagctaccc cgatctgcaa attcagttcg gtttgatgag tcgccaagag
    12001 gtgtggagct atatcgaatc ggcccgattc acgatgacgg cagtgcagga agccgtgcag
    12061 accaagacga cgacccgccc gccgaagcta tccgaactga agccgcgagc ggacgctcct
    12121 cctctttagg atcggaaggc cgtacaattt gctgacacgt tctgtacgaa ggcaactctg
    12181 atggctctgg caactctcat agcgatcacc ctcgggtgta tcggctggag tttgtggatc
    12241 cgccgcgtca cgtggtcgtg ccggtgggag gtcgccgcga ccctgaacat cgctttgcag
    12301 ggtcttgccg tcatactcat gtccccgttg gcctcggaaa cgctcggcca ttggctgcat
    12361 gcgctgaccg gcatgtggaa tctcgaggac tacatcggac acgacgccta catcgtcgcc
    12421 gcctccgcag tcgtctacaa cgcgctcggg cgcctcgccg acgaccacgc catgcagacc
    12481 tcgttcaagc agtatgtgga gcgtcctgcg acgctctgca tcccactgtt gctggtgacg
    12541 ttctcgatcg gcaacggcgc aagcgtgtac cggtccgact tcttcgaggt gcccaccgac
    12601 ttctggctca acatgtactg gctgctgctc tgcggcatgc tgatctacct gctgacctac
    12661 ggtatgcggg cgctgatgat cctccgacag gatccccggt cgcggaagat cgccaacatc
    12721 tacctcgtcg catcagccag cgggatcatg gcgtgcaccg tgcggatcat caccgcctac
    12781 gtgcccgcgc tgcagacggt cgagggcggc acgctggtgt ggattttcgc ctgtggctgt
    12841 ggcgccggat tcgcgctgac gtccgcgcag tcctggcgca tcaagaccaa gtggttcacc
    12901 cgcgccagta ggtagttagc ccgcgacggg cctgggcacg tcgtatggcg cgccgaacgc
    12961 cggcatcgtc tcgtagcccc gcttgcgggc ctgcgccgag tcgcggcgga tcagggcatg
    13021 cagaccggca aacgttgcct ggtcgagcag catgcgggtg agcaaccggt tgtgcacgaa
    13081 cccgaacgac agccccgtcg acgggtcggc ccagcccacg gagccggcca gccccgcgtg
    13141 acc
//
LOCUS       DASRJA010000193         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1929899,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000193 DASRJA010000000
VERSION     DASRJA010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929899"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1405)
                     /locus_tag="VFC01_08625"
     CDS             complement(<1..1405)
                     /locus_tag="VFC01_08625"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZN79735.1"
                     /translation="MRLPYSWLRDVVQAGAPGWDVSADDLEQTLIRIGHEVEEVIPVG
                     PVTGPLIIGRVLEIEELTEFKKPIRAVKVDVAEAEPRDIVCGATNFAVGDLVVVALPG
                     TVLPGDFTIATRKTYGRISDGMICSTAELHLGTDHSGILVLPPGTAEPGTAAADVLGL
                     DDVVFHLAITPDRGYCLSVRGMAREIACAYDLDYVDPADVPPLPAEGEALPVTIQPGT
                     GVLRFGLRPVIGIDPNALSPWWMQRRLLLSGIRAISPAVDVTNYVMLELGHPMHAHDH
                     SLITGGFKVRFAEPGETVVTLDDIERKLDPRDVLIVDDVATAAIGGIMGAGTTEVRDT
                     TTDLMLEAAVWDPAAVSRTARRLHLTSESGRRYERTVDPAVSVAALDRAATLLADIAG
                     GTIEPKLTDWRGDPPREDWSQPPVSMAVDLPDRIAGVDYADGTTRRRLTQIGAEVATD
                     GDQLTATPPSWRPDLREP"
     gene            complement(1405..>2103)
                     /gene="pheS"
                     /locus_tag="VFC01_08630"
     CDS             complement(1405..>2103)
                     /gene="pheS"
                     /locus_tag="VFC01_08630"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZN79736.1"
                     /translation="HPITILADHIADTFVAMGWELAEGPEVETEQFNFDALNFPPDHP
                     ARSEQDTFYIAPDESRQVLRTHTSPVQIRALLERDLPVYIISIGRTFRTDELDATHTP
                     VFHQVEGLAVDKGLTMAHLRGTLDAFARSEFGPQGRTRFRPHFFPFTEPSAEVDVWFA
                     NKKGGPGWVEWGGCGMVNPNVLRACGIDPEVYSGFAFGMGLERTLQFRNGIPDMRDMV
                     EGDVRFSLPFGVGA"
BASE COUNT          333 a          683 c          747 g          340 t
ORIGIN      
        1 cgggctcgcg caggtcgggc cgccagctcg gcggggtggc cgtcaattgg tcgccgtcgg
       61 tcgccacctc ggcgccgatc tgggtcagcc tgcgccgtgt ggtgccgtcg gcgtaatcga
      121 cgcctgcgat gcggtccggc aggtcgacgg ccatgctgac tggaggctgc gaccagtcct
      181 cgcgtggcgg gtcaccgcgc cagtcggtca gcttgggttc gattgtgccg cccgcgatgt
      241 cggcgagcag agtggcggct cgatcgaggg cagcgaccga tacggccgga tccaccgtcc
      301 gttcgtagcg cctgcccgat tcgctcgtga gatgcaggcg acgcgcggtg cgcgagaccg
      361 cggcgggatc ccacaccgcc gcctccagca tcaggtcggt ggtggtgtca cgcacctcgg
      421 tggtgcctgc acccatgatg ccgccgatcg cggcggtcgc gacgtcgtcg acgatgagca
      481 cgtcacgagg gtcgagcttg cgctcgatgt cgtcgagcgt gacgactgtc tctcccggct
      541 ccgcgaagcg aaccttgaag ccgccggtga tcaggctgtg gtcgtgggcg tgcatcggat
      601 gaccgagctc gagcatcacg taattggtga cgtccacggc cggggaaatc gcgcggatgc
      661 cgctcagcaa gagtcggcgt tgcatccacc acggcgacag cgcattcggg tcgatgccaa
      721 tgacgggccg cagaccgaat cgcagcacgc ccgtgccggg ctggatggtc accggcaggg
      781 cctcaccctc ggcgggcagc ggtggtacat cggcggggtc gacgtagtcg agatcgtagg
      841 cgcaggcgat ttcgcgcgcc atgccgcgca ccgacaggca gtagccgcgg tccggcgtga
      901 tcgccaggtg gaacaccaca tcgtcgagcc cgagcacgtc ggccgccgcg gttcccggtt
      961 ccgcggtgcc cggcggaagg accaggatgc cggaatggtc ggtgcccaag tggagttcgg
     1021 ccgtcgagca gatcatcccg tcggatatgc ggccgtaggt cttgcgggtt gcgatcgtga
     1081 agtcccccgg cagcaccgtg ccaggcagcg cgaccacgac cagatcgccg acagcgaagt
     1141 tcgttgcccc gcagacgatg tcacgcggct cggcctcagc gacgtcgacc ttgacggcgc
     1201 ggatcggctt cttgaactcg gtgagttctt cgatctcgag gacccgaccg atgatgagcg
     1261 gtccggtcac cggtccgacg ggaatgacct cttcgacctc gtgaccgatg cggatcagcg
     1321 tctgctccag gtcgtcggcg gacacgtccc atccgggagc gccggcctgg acgacgtcgc
     1381 gaagccagct gtagggaagc cgcatcaggc tcctaccccg aacggcagcg agaagcggac
     1441 gtctccctcg accatgtcgc gcatgtcggg aatgccgttg cggaactgca gcgtgcgctc
     1501 cagtcccatg ccgaacgcga agccggagta gacctcgggg tcaatgccgc aggcgcgcaa
     1561 cacattcgga ttgaccatgc cgcagccgcc ccactccacc cagccggggc cgcccttctt
     1621 gttggcgaac cagacgtcga cctcggccga gggttccgtg aacgggaaga aatgtggccg
     1681 gaatcgggta cggccctgcg gaccgaactc cgagcgtgcg aaggcgtcga gtgtgccgcg
     1741 cagatgggcc atcgtcaggc ccttatccac ggccaggccc tccacctggt ggaagacggg
     1801 ggtatgcgtc gcgtcgagct cgtcggtgcg gaacgtccgg ccgatggaga tgatgtacac
     1861 cggcaggtcg cgctcgagca gcgcgcggat ctgcaccggc gacgtgtggg tacgaagcac
     1921 ctgccgcgac tcgtccgggg cgatgtagaa cgtgtcctgc tcgctgcgcg ccgggtggtc
     1981 aggcgggaag ttcagcgcgt cgaagttgaa ctgctccgtc tcgacctcgg ggccttcggc
     2041 cagctcccag cccattgcga cgaacgtgtc ggcgatgtgg tcggcgagga tcgtgatcgg
     2101 gtg
//
LOCUS       DASRJA010000194         8498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3816145,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000194 DASRJA010000000
VERSION     DASRJA010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8498
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3816145"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /gene="dnaK"
                     /locus_tag="VFC01_08635"
     CDS             complement(<1..1373)
                     /gene="dnaK"
                     /locus_tag="VFC01_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZN79737.1"
                     /translation="MARAVGIDLGTTNSVVAVLEGGDPVVIANSEGSRTTPSVVAFAR
                     NGEVLVGQPAKNQAVTNVDRTIRSVKREMGTDWSVEIDGKKYTAQEISARVLMKLKRD
                     AEAYLGEDITDAVITTPAYFNDAQRQATKEAGQIAGMNVLRIVNEPTAAALAYGLDKG
                     EKEQTILVFDLGGGTFDVSLLEIGEGVVEVRATSGDNHLGGDDWDQRIVDWLVDKFKG
                     TSGIDLTKDKMAMQRLREAAEKAKIELSSSQSTSINLPYITVDADKNPLFLDEQLTRA
                     EFQRITQDLLDRTRNPFKSVIADTGISVSDIDHVILVGGSTRMPAVTDLVKELTGGKE
                     PNKGVNPDEVVAVGAALQAGVLKGEVKDVLLLDVTPLSLGIETKGGVMTKLIERNTTI
                     PTKRSETFTTADDNQPSVQIQVYQGEREIASHNKLLGSFELTGIPPAPRGVPQIEVTF
                     DIDANGIV"
     gene            complement(1597..3102)
                     /locus_tag="VFC01_08640"
     CDS             complement(1597..3102)
                     /locus_tag="VFC01_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN79738.1"
                     /translation="MTTAPQEHVYDNSALLAEDEGYHKGLKPRQLQMIAIGGAIGTGL
                     FLGAGGRLHNAGPGLFLSYAFCGIFVFLILRALGELVLHRPSSGSFVSYAREFLGEKA
                     AFVAGWMYWLNWAFTAIVDCTAIATYFHYWSAFGSVPQWLLALLALAAVVSVNLISVT
                     LFGELEFWAAAIKVLALMTFLVAGTIFLAGRYDIQGQSTGFSVIADNGGLFPTGLFPL
                     VIVMSGVVFAYAAVELVGTAAGETAHPHKIMPRAINSVIARIAVFYVGSLFLLALLLP
                     YTTYKAGESPFVTFFGKIGVEGAGTAMNIVVLTAAFSSLNAGLYSTGRILRSMAMNGS
                     APKFTAVMSSRGVPYGGIFLTAAVGLFGVILNLLFPGEAFEIVLNIAALGIIASWGTI
                     VICQIQLYRWSKRGILERPSFRMWGTPYTGYLTLAFLFGVVVLMALDPPIGTWTVATL
                     VVIIPALIIGWYAVRGRVLEIAREREGFTGQFPVVANRPLPRDKDDEDKDK"
     gene            complement(3123..3374)
                     /locus_tag="VFC01_08645"
     CDS             complement(3123..3374)
                     /locus_tag="VFC01_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79739.1"
                     /translation="MARVDLFAKIPLIGMSLVFLAMFIVAVTAYLHIGWYSIIGYAIA
                     AVVAVFGFVMTFRDVRDTPGPAEKWDVKGTPIDPASPQN"
     gene            3555..4274
                     /locus_tag="VFC01_08650"
     CDS             3555..4274
                     /locus_tag="VFC01_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN79740.1"
                     /translation="METKVDEIGPDVFRLSTWVPDVTEHGFTFNQFLLTGDEPFLFHC
                     GHRQLFPLVSEAINRVVPLEKLRWISFGHLEADECGAVNLLLGAAPNAEVIHSALACL
                     VSLNDLCDRPPVLAPEDGVHDTGGHRLRFIATPHVPHNWEAGLWFDEATSTLLAGDLL
                     THTGQCPALTESDCVAPALEAEEIFHATGLTTNLGPTLEQLAELNPTTLAIMHGSSYS
                     GDGAAQLRALADGYAEMVGAA"
     gene            4271..4891
                     /locus_tag="VFC01_08655"
     CDS             4271..4891
                     /locus_tag="VFC01_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HZN79741.1"
                     /translation="MTAAQRERAALVETMRAVGPDAPTLCGDWTTRDLAAHLVVREGR
                     PDATAGVAIPFLAGYTAKVQRQVTESTDWEKLLDKIASGPPLYSPFKIIDPIANLGEM
                     YIHHEDVRRAQTGWEPRQLDDGLVKALGRGLSIMARLTLAKAPARVTLRTPQGKEVVA
                     LGRGPELTVTGEPQELLLFLSGRDEVRLTFSDAQVAQTVRANRRGL"
     gene            complement(4893..5660)
                     /locus_tag="VFC01_08660"
     CDS             complement(4893..5660)
                     /locus_tag="VFC01_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79742.1"
                     /translation="MTITAIRPRTDGEAAVLVRIALFVTWASMPLLAIVADVFGLVPM
                     GTSAIIVVPLSVMLLVLMKVAPHPSDAIIRHGFIVGMVACVPYDVFRLSAVHLGHLMG
                     DFIPKLGLFIMGDSGAVGATVGYLWRYLGDAAGVAVGFYVVAFMVGLHRWSRPGRVVL
                     AATAFAVFPVWTGLISLVALAPRGEELMFELTPATVAITLIGHMIFGFVLGLGFVRAR
                     HLDAYWPWPPLSLTALRRDAAPADVHQLTKPRLAEAA"
     gene            5916..6848
                     /locus_tag="VFC01_08665"
     CDS             5916..6848
                     /locus_tag="VFC01_08665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN79743.1"
                     /translation="MDFRVFVEPQQGATYSDQLAVARAAEALGYSAFFRSDHYVAMSG
                     DGLPGPTDSWVTLAGIARETSTIRLGTMVTSATFRYPGPLAISVAQVDEMSGGRVELG
                     IGAGWFEAEHQAYAIPFPSLGERFDRLTDQLHIITGLWTTPAGDTFNYEGKHYTVTES
                     PALPKPAQSPHPPIIIGGGGAKRTPALAAQFAAEFNIPFAPFDVAKTQYDRVATALSD
                     AGRQPDSLTYSAAFVVCAGRDDAEIARRAAAIGREVDELRSNSPTVGTPAEIADKLGA
                     YIELGVQRVYLQLLDLSDLDHLEFFATQVIPQFG"
     gene            complement(6911..7309)
                     /locus_tag="VFC01_08670"
     CDS             complement(6911..7309)
                     /locus_tag="VFC01_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79744.1"
                     /translation="MRVTFVRGPRRVVLGADPSLLGRLLVTRVDVEGSLLLDVSDEDE
                     VVADGALMSVVTGGLVLVMVWVVVGVDDPVAVAENDGSTKFSGACVGSLNWPATKVTA
                     AMRMPITARPATLAPMTLGVRLYHGVCSSS"
     gene            7348..7809
                     /locus_tag="VFC01_08675"
     CDS             7348..7809
                     /locus_tag="VFC01_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79745.1"
                     /translation="MARYGPPDDPNNPDNQPTAYINYGDYGQQPPGEPLPWYRKPVAL
                     VAFGAVGAVLIALFIFGLVKLLTGGTSQEAPTTLTPITRTTTSATAPTTTTATTEPTT
                     TEQTTTTTEPTTTTTTTTTTTTMTEPSVSTSVSTSTSTSTVTQTVTVPPPP"
     gene            complement(7900..>8498)
                     /locus_tag="VFC01_08680"
     CDS             complement(7900..>8498)
                     /locus_tag="VFC01_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Isoniazid-inducible protein iniC"
                     /protein_id="HZN79746.1"
                     /translation="PVDAATRAALLDRFGMFGIRISIAVLRAGVSDSVALADELLERS
                     GLVALRDVIDQQFAQRSDLLKAHTALLSLRRFVETNPIYATRYIIADIDPLLADTHAF
                     EELRLLSALRSRPTTLNEDEMASLRRIIGGSGTDAASRLGLQPDAPYDGPRAAFAAAQ
                     RWRRRADHPLNDPFTTRACRAAVRSAEAMVAEYAARGR"
BASE COUNT         1595 a         2831 c         2681 g         1391 t
ORIGIN      
        1 acgatgccgt tggcgtcgat gtcgaaggtg acctcgatct gcggcacgcc acgcggggcc
       61 ggcgggatgc cggtcagctc gaagctgccg agcagcttgt tgtgcgaggc gatttcgcgc
      121 tcaccctgat agacctggat ctgcaccgac ggctgattgt cgtcggcagt ggtgaaggtc
      181 tccgaccgct tggtcgggat cgtggtgttg cgctcgatca gcttggtcat cacaccaccc
      241 ttggtctcga tgccgaggga cagcggcgtg acatcaagca gcagaacgtc tttcacctcg
      301 cccttgagca cgccggcctg cagcgcggcg cccaccgcga cgacctcatc cgggttgacg
      361 cccttgttgg gctccttgcc gccggtgagt tccttgacca ggtcggtaac cgcgggcatg
      421 cgcgtggaac caccgaccag gatcacgtgg tcgatgtcgg acaccgagat gcccgtgtcg
      481 gcgatcaccg acttgaacgg gttgcgggtg cggtccagca ggtcctgtgt gatgcgctgg
      541 aactcggcgc gagtcagctg ctcgtcgagg aacagcgggt tcttgtctgc gtcgacggtg
      601 atgtagggca ggttgatcga ggtgctctgc gagctggaca gctcgatctt ggccttctcg
      661 gcggcctcac gcagccgctg catggccatc ttgtccttgg tcaggtcgat gccgctggtg
      721 cccttgaact tgtcgacgag ccaatcgacg atgcgctgat cccagtcgtc gccgcccagg
      781 tggttgtctc ccgaggtggc gcggacctcg acaacgccct caccgatctc cagcagcgag
      841 acgtcgaagg tgccgccacc gaggtcgaac accaggatgg tctgttcctt ctcgcccttg
      901 tccagcccgt aagccagcgc cgccgcggtg ggctcgttga cgatgcgcag cacgttcatg
      961 ccggcgatct ggccggcctc cttggtggcc tggcgctggg cgtcgttgaa gtaggcgggc
     1021 gtggtgatca ccgcgtcggt gatgtcctcg ccgaggtagg cctcggcgtc gcgcttgagt
     1081 ttcatcagca cgcgagcgct gatctcctgc gcggtgtact tcttgccgtc gatctccacg
     1141 gaccagtcgg tgcccatttc acgcttgacc gaccgtatgg tgcggtcgac gttggtcact
     1201 gcctggttct tggcgggctg gccgaccagc acctcaccgt tgcgcgcgaa cgcgacgacg
     1261 gacggcgtgg tgcgggagcc ctcggagttg gcgatcacaa cggggtcgcc accctcgagc
     1321 accgcgacga cggagttggt ggtcccgagg tcgattccga ccgcacgagc catggttttg
     1381 cctcctgaaa tagacgtact aggggtctga gtgcaccgca ctcaagactg ctccggccga
     1441 cattaccctg tcaacccaga cttgagcgtg ctgaactcaa ctgtcggatt agttaacggc
     1501 aggtcggcgc gatttgttcc catccatacc gccgttaggg tcggccatct ggtcggtccc
     1561 ggtaaaccgg ccggtcagct agacggaatg actaggtcat ttgtccttgt cttcgtcatc
     1621 cttgtcgcgt ggcagcgggc ggttggcgac gaccgggaat tggccggtga agccctcgcg
     1681 ttcccgagcg atctccagca cccgcccgcg aaccgcatac caaccgatga tgagcgccgg
     1741 aatgatgacc accagcgtgg cgacggtcca ggtgccgatc ggcgggtcga gcgccatcag
     1801 cacgacgacg ccaaacagga acgccaacgt gagatagccg gtgtacggcg tgccccacat
     1861 ccggaatgac ggccgctcca ggatgccgcg tttcgaccag cggtagagct ggatctgaca
     1921 gatgacgatg gtgccccagg acgcgatgat gccgagggcc gcgatgttga gcacgatctc
     1981 gaacgcctcg ccggggaaga gaaggttgag gatgacaccg aacagcccga cggctgcggt
     2041 caggaagatt ccgccgtacg gcacgcctcg gctggacatc accgccgtga acttcggtgc
     2101 gctgccgttc attgccatcg accgcaggat ccggccggtc gagtacaacc cggcgttcag
     2161 gctggagaat gcggcggtaa gcacgacgat gttcatggcc gtgcccgcgc cctcgacccc
     2221 gatcttgccg aagaacgtga cgaacgggct ctcgccggcc ttgtaggtgg tgtacgggag
     2281 aagaagggcc agcaggaaca gtgagccgac gtagaagaca gcgatacgtg cgatcaccga
     2341 gttgatcgcg cgtggcatga tcttatgcgg atgcgcggtc tcgccagccg ctgttccgac
     2401 gagttcgact gcggcgtaag cgaacacgac gccggacatg acgatcacca atgggaagag
     2461 gccggtggga aaaagcccac cgttgtcggc gatcacgctg aaaccggttg actgcccttg
     2521 aatgtcgtag cggccggcaa gaaagatggt gcctgcgacc aggaatgtca tcagcgcaag
     2581 gactttgatg gccgcagccc agaactcgag ctccccgaac agggtaaccg aaatgaggtt
     2641 tacgctcacc acggccgcca gcgcgagcag tgcgagcagc cactgtggga cgctcccgaa
     2701 cgccgaccag tagtggaaat aggtggcgat cgcggtgcag tcgacaatgg ccgtgaacgc
     2761 ccagttcagc cagtacatcc agccggcgac gaacgccgcc ttctcaccga ggaattcgcg
     2821 cgcatacgac acgaaggacc ccgatgacgg ccggtgcagc acgagttcgc ccagcgcccg
     2881 caggatcaga aagacgaaga tgccgcagaa cgcatagctc aagaacagcc ccggtccggc
     2941 gttgtgtagt cggccgccgg caccgaggaa cagtccggtg ccgatggcac cgccgatcgc
     3001 gatcatctgc agttgccggg gctttaggcc cttgtggtag ccctcgtcct ccgccagcag
     3061 cgcggagttg tcatacacat gttcctgggg tgcggtggtc atcgaaccag tcctcgctct
     3121 agtcagtttt ggggtgacgc aggatcgatg ggggtgcctt tgacatccca cttctccgca
     3181 ggtcctggcg tatccctgac gtcacggaac gtcatgacga agccgaacac ggcgacgacc
     3241 gccgcgattg cgtagccgat gatcgagtac caaccgatat ggaggtacgc ggtgaccgcg
     3301 acgatgaaca tcgccaggaa caccagggac atcccgatca atggaatctt tgcgaacaaa
     3361 tcaacgcgtg ccatgagacc cacctcgata gaccgaatga cggtgataac agtgaacacc
     3421 ttccggcgtt gtcgtgctcg ccaaatggaa ctacgtggtg ttcgggtggg cggtgatcgc
     3481 cgccgttatc gcggtggcgg gatttgccgt cgcattcgac gacttgacct gacgacgagg
     3541 cgcacgatgg actcatggag acgaaagtcg acgagatcgg gcccgacgtg ttccggcttt
     3601 ccacttgggt tccggacgtc accgagcacg ggttcacgtt caaccagttc ctgctgaccg
     3661 gcgacgagcc gttcctgttc cactgcggtc atcgccagct gttcccgttg gtttcggagg
     3721 ccatcaaccg ggtcgtgccg ctggaaaagc tgcggtggat ctcgttcggc catctcgagg
     3781 ccgacgagtg cggagcggtg aacctgttgc tgggcgctgc cccgaatgcc gaggtcatcc
     3841 acagcgcgtt ggcgtgcctg gtgtcgttga acgatttgtg cgaccgcccg cctgtgctgg
     3901 cacccgagga tggcgtccac gacaccggcg gccaccgact gcggttcatc gccaccccac
     3961 acgtcccgca caactgggaa gccggactct ggttcgacga ggcgacgtcg accctgttgg
     4021 ccggtgacct gctcacccac accgggcaat gtcctgcttt gaccgaatct gactgcgtcg
     4081 caccggcatt ggaagccgag gagattttcc acgcaacagg ccttacgacg aacctcgggc
     4141 cgacgctaga gcaactcgcc gagctgaacc cgactacgct ggcgatcatg cacggctcgt
     4201 cgtactccgg cgacggcgcc gcgcagctgc gggcgctggc cgacggttat gccgagatgg
     4261 tgggcgccgc atgaccgcgg ctcagcgcga acgcgccgca cttgtcgaaa ccatgcgcgc
     4321 cgtcggccct gatgcgccca cgctatgcgg ggactggacg acccgtgacc tcgccgcgca
     4381 cctggtggtt cgggaaggcc ggccggatgc cactgcgggg gttgcgattc cgtttctcgc
     4441 cgggtacacg gccaaggttc agcgtcaggt caccgagtcc accgactggg agaagctgct
     4501 ggacaagatc gcctccggcc cgccgctcta ttcaccgttc aagatcatcg acccgatagc
     4561 gaacctgggc gagatgtaca tccaccacga ggatgtccgc cgcgcgcaaa ccggttggga
     4621 gccaaggcag ctcgacgacg ggctggtaaa ggccctgggc cggggacttt cgatcatggc
     4681 aaggctcacg ttagccaagg ccccggcgag ggtgaccctc cgcactccgc agggtaaaga
     4741 ggtcgttgcc ctgggccgcg ggcccgagct caccgtgacc ggcgaaccgc aggaactgct
     4801 gctcttcctc tctggtcgcg acgaagtgcg gctgacattc tccgatgccc aggtcgccca
     4861 gacggttcgc gccaaccgcc gcggcctgtg actcaggccg cctctgccaa ccgtggcttc
     4921 gtgagctgat gcacgtccgc gggcgctgcg tcgcggcgca gtgcggtaag tgaaagcggc
     4981 ggccacggcc agtacgcgtc gaggtggcgg gcacggacga agcccagccc cagtacgaag
     5041 ccgaagatca tgtgaccgat caacgtgatc gcaacggtgg ccggcgtgag ctcaaacatc
     5101 agttcttccc cacgcggagc cagggccacc aggcttatca gaccggtcca caccgggaac
     5161 actgcgaagg cggtggccgc cagcaccacg cgaccggggc gcgaccaccg gtgcagaccc
     5221 accatgaacg cgacgacata gaagccgacc gcaacgccgg cggcatcgcc caggtagcgc
     5281 cacaggtagc cgaccgtggc acccacagca ccggaatcac ccatgatgaa caaccccagc
     5341 ttggggatga agtcgcccat caaatggccc agatgcactg cgctcaaccg aaacacgtca
     5401 taggggacgc aggcgaccat ccccacgatg aacccgtggc gaatgatggc gtcgctcgga
     5461 tgcggcgcca ccttcatcag cacgagcagc atcacgctca gcgggacaac gatgatcgcc
     5521 gaggtgccca tcggcaccag gccgaagacg tccgcgacga tcgccagcaa cggcatggac
     5581 gcccaggtga caaacaacgc gatacgtacg agaacagccg cctcgccatc ggtccggggc
     5641 cgaattgccg tgatagtcaa gctgatcctc ctgttgatgc tttggtgcgg tgacttccgc
     5701 gtgcccaaaa gtgtcggtgt gcgggcttgg aggatcctca agagtttctt gggactgcga
     5761 tttgctggcg ttttcctact cgccgcggcg atcggggtct ctgccgcggc cccggcagag
     5821 actgaaccac tggcgccgga ccgcaccggc catggcatgt cggtcagcgt cgacgaggtc
     5881 agcacgtacc aggcagcgcc tctaggctgg cggtcgtgga tttccgcgtc ttcgtcgaac
     5941 cccagcaagg tgccacctac tcggatcagc tcgcggtcgc ccgcgccgcc gaggcgctgg
     6001 gatattcggc cttctttcga tccgatcact acgtcgcgat gagcggcgac ggcctgccgg
     6061 gaccgaccga ctcctgggtg acgctcgccg gcatcgcgcg cgaaacgtcg acgatccggc
     6121 tcggaaccat ggtcacctca gcgaccttcc gctacccggg gccgctggcg atctcggtcg
     6181 cccaggtcga cgaaatgagc ggcggccgtg tggaattggg tatcggcgcc ggctggttcg
     6241 aagccgaaca tcaggcctac gcgatcccat tcccgtcgct gggcgagcgc ttcgaccggc
     6301 tcaccgacca gctgcacatc atcaccgggc tgtggacgac accggccggg gacaccttca
     6361 actacgaggg caagcactac accgtcaccg aatccccggc gctgcccaaa cccgcgcagt
     6421 cgccacatcc gccgatcatc atcggtggcg gtggcgccaa acgaacgccc gcccttgccg
     6481 cgcagttcgc cgcagaattc aacatcccgt tcgcaccatt cgacgtggcc aagacccagt
     6541 acgaccgggt cgccaccgcg ctcagtgacg ccgggcggca acctgactcg ctgacgtatt
     6601 cggcggcctt cgtggtctgc gcgggacgcg acgacgcgga gatcgcgcgg cgcgcagcgg
     6661 cgatcggccg tgaggtggac gaactgcgca gcaactcccc gacggtggga acacccgcgg
     6721 agatcgctga caagctcggc gcatatatcg agctgggcgt gcagcgggtt tacctgcagc
     6781 ttctcgactt gtcggacctc gaccacctgg agttcttcgc cacccaggtc atcccgcagt
     6841 tcggctgacc ggcgtcgtcg cgccggccac ggctactatc actggccatg gccgacgccg
     6901 accatgacga ctacgacgac gagcagacgc cgtggtacaa ccgcacgccc aacgtcatcg
     6961 gggccagcgt ggccggcctg gccgtgatcg gcattctcat cgccgccgtg actttcgtcg
     7021 cgggccagtt caatgagccc acgcaagcgc cactgaactt cgtcgagcca tcgttttcgg
     7081 cgaccgccac cgggtcgtcg acaccgacca ccacccagac catcaccagc accagccccc
     7141 cggtcaccac cgacatcaac gcaccgtcgg ccaccacctc gtcctcgtcc gacacgtcca
     7201 gcagcagtga accctcgacg tcgactcggg tcaccaacag ccgtccgagc aacgacggat
     7261 cggcgcccag tacgacgcga cgcggcccac gcacgaatgt gacacgcacg ctctatccgc
     7321 agccgtaacc gcctactatc agtgcccgtg gcgcgctacg gacctcccga cgacccgaac
     7381 aaccccgaca accaaccgac tgcctacatc aactacgggg actacgggca gcaaccgccg
     7441 ggtgagccgc tgccctggta tcgcaaaccg gtggcgctgg tggcattcgg cgcggtcggc
     7501 gcggtgctga tcgccttgtt catcttcggt ctggtcaagc tgctcaccgg cggcacgtca
     7561 caggaagcgc cgaccacgct cactccgatc acccggacca cgacgtcggc gaccgcgccc
     7621 acgacgacca ccgcgacaac ggagccgacc accaccgagc agaccacgac cacaaccgag
     7681 cccacgacca ccacaaccac gacgaccaca accacgacga tgaccgaacc gagcgtgagt
     7741 acgagcgtca gcacgagcac aagcaccagc acggtcaccc agaccgtcac agtgcccccg
     7801 ccaccctgac gtgcccggta gcgcatccag ttcactctgt gtgcagggcg caaaagtgcg
     7861 agagacttgc gccctcagcg cagagtcaac gaaagcgaat tagcgtccgc gagcggcata
     7921 ttcggcgacc atagcctccg cgctgcgcac cgccgcccgg cacgctcgcg tggtgaacgg
     7981 gtcgttgagg ggatggtcgg cgcggcgccg ccaccgctgg gccgccgcga acgccgcccg
     8041 cggcccgtcg taaggcgcat cgggctgcag gccaagcctg ctcgccgcgt cggtaccgga
     8101 gccaccgatg atgcgtcgca gcgaagccat ctcgtcctca ttcaaagtcg ttgggcggga
     8161 acgcaaagcg ctcaacaacc gaagttcttc aaacgcgtgg gtgtcggcaa gcagcgggtc
     8221 gatatcggcg atgatgtatc gcgtcgcata gatcgggttg gtctccacga agcgccgcaa
     8281 cgacagcagc gcggtgtgcg ccttgagcag atccgaacgc tgcgcgaact gctggtcgat
     8341 cacgtcgcgc agcgccacca gcccactgcg ctccagcagc tcgtcggcca gcgccaccga
     8401 atcgctgaca ccggcgcgca gcaccgcgat cgagatcctg atgccgaaca tgccgaaccg
     8461 gtccagcagc gcggctcggg tggcggcgtc gaccggca
//
LOCUS       DASRJA010000195         3388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3056203,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000195 DASRJA010000000
VERSION     DASRJA010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3056203"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VFC01_08685"
     CDS             complement(<1..1217)
                     /locus_tag="VFC01_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN79747.1"
                     /translation="MLVGREKELAAIQHALHGLGGARQLQRSRFLEVVGDPGIGKTSL
                     LDVLRAEAEARGWWTLRGCATEFETEVPLYALVDAMDDTLSGIRPSLSASLVSALGTV
                     FPALFDSNAAAETTMRERYRLHRSIGQLLEHIAAAGPVVVILDDMQWADPALVDLLAH
                     LTRHPPRARVLIALAYRPRQAPARLVDALATAPDGLVDRRELGPLTAEQTGELLGAAV
                     APSRVQELHDHSGGNPFYLQALARAAIDAPALTARRSLEPLGGEMPATVRAALLTEMD
                     GLSATARLVADIAAVAGDPFEPDLVADVAELDATRVQAAIDELVERDLIRPESPDARF
                     GYRHPLVRHVAYNATGPAWRCGAHGRVARALERRGAPLVLRAHHVARSARPGDTSAVE
                     LLAAAARDTLARAP"
     gene            1489..1914
                     /locus_tag="VFC01_08690"
     CDS             1489..1914
                     /locus_tag="VFC01_08690"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN79748.1"
                     /translation="MSTPTQPQSGADVMAQFIPSSPFVAKLGIVAEVLDGDEVRLRLP
                     WDPTNVTIADMVHGGAIAALADVAVMAAAWVGADVPDSLRGVTTSMSMQFLAPARATD
                     LIAIGRVLKRGKTLVNCDVDVVTPEGEAVAKAIATYKIG"
     gene            complement(1911..2402)
                     /locus_tag="VFC01_08695"
     CDS             complement(1911..2402)
                     /locus_tag="VFC01_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZN79749.1"
                     /translation="MSVTDEYLKNNEAYASTFTGPLPLPPSKHVAVVACMDARLDVYR
                     VLGLGDGEAHVIRNAGGVITDDEIRSLAISQRLLGTKEIILIHHTDCGMLTFTDDEFK
                     RAIQDETGIKPEWAAEAFPDPEEDVRQSLRRIEASPFVTKHESLRGFIFDVATGRLNE
                     VTL"
     gene            2606..3142
                     /locus_tag="VFC01_08700"
     CDS             2606..3142
                     /locus_tag="VFC01_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79750.1"
                     /translation="MSKRLRAIGDPVVADAPAGARIRTRIHPTAAEAETLAAIGELLG
                     SVYRSELAERVRLGRLDRKAHALWRAERKRALTAGSSSRWAGAITRAVEDQYQLGTRG
                     LAAQVEDLRAAGEMLEQRCALRPDELAPVETTDPQARRRRRGYRNSAERFTKTRRLAV
                     LRGRLATAEEALGRVGRR"
     gene            3197..>3388
                     /locus_tag="VFC01_08705"
     CDS             3197..>3388
                     /locus_tag="VFC01_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79751.1"
                     /translation="MTEQRWREKWDAARLFLTADGESGKAGGNETIRVDEHGQLRIKV
                     PGALADELGTHLVIAAPVQF"
BASE COUNT          545 a         1111 c         1203 g          529 t
ORIGIN      
        1 ggcgcccgag ccagcgtgtc ccgggccgcc gctgcgagca gctcgacagc cgacgtgtct
       61 ccagggcggg ccgaacgggc cacgtgatgg gcacgaagca ccagcggcgc gccacgtcgc
      121 tccagcgccc gcgcgacccg tccgtgcgcg ccgcagcgcc aggccgggcc ggtcgcgttg
      181 taggccacgt ggcgcaccag gggatgccgg tatccgaagc gtgcgtcggg gctttcggga
      241 cggatcaggt cgcgttcgac gagctcgtcg atggcggcct gcacccgcgt cgcgtcgagc
      301 tcggcgacgt cggccacgag gtcgggctcg aacggatcgc cggcgactgc ggcaatatcg
      361 gcgactaggc gtgccgtcgc ggacagcccg tccatttcgg ttagcaacgc ggcccgcacg
      421 gtggccggca tttctccgcc gagcggctcg aggctcctgc gcgctgtcaa tgctggggcg
      481 tcgatggcag cacgggccag cgcctggagg tagaagggat tgccaccact atggtcgtgc
      541 agctcctgga cccgggacgg agcgaccgcg gctccgagca gttctcctgt ctgctcggcc
      601 gtaagcgggc cgagctcgcg ccggtcgacg agcccgtcgg gtgcggtggc cagcgcgtcc
      661 accaggcgcg ccggggcctg acgcggacgg taggcgagcg cgatcagcac tcgggcgcgg
      721 ggcgggtgcc gtgtgaggtg ggccagtagg tcgaccagcg ccgggtcggc ccattgcatg
      781 tcgtccagga tgacgacgac cggcccagcg gcggcgatgt gctcgagcag ttggccgatc
      841 gaacggtgca gccggtaacg ctcgcgcatc gtcgtctctg cagccgcgtt ggagtcgaac
      901 agtgccggga acacagtccc gagcgccgac accagcgacg ccgacaggct cggccggata
      961 ccgctcaagg tgtcgtccat cgcgtcgacg agggcgtaga gcggcacttc agtctcgaac
     1021 tcggtggcgc aacctcgcag cgtccaccag ccgcgcgcct cggcctcggc tcgcagaacg
     1081 tcgagcaggc tggtcttgcc gataccgggg tcgccgacga cctccaggaa tcgcgagcgc
     1141 tgcagttgcc tcgcaccgcc tagaccgtgg agagcgtgct ggatcgcggc cagctctttc
     1201 tcgcgtccga ccaacatctc cgcttccacg attctgcaca gacccgggtg taccggctcg
     1261 ttacgtcagg aacgtaatgg cctggggcac cgaaaaacac gagtagcgaa ctacctgtct
     1321 tgtggttccg agtaggaccc gcccgcccag acaagagacc gtggggctga acgatgttga
     1381 cggacagcga ctttggtgcc gggtgcccgg tgctagcaac ctcaattgcg gacctcgtcg
     1441 ctcccctctt accccagagg taattgatcc accgcacata ctggagccat gtcgaccccc
     1501 acccagcccc agtcgggcgc ggatgtcatg gcccagttca tcccgtcgtc cccgttcgtc
     1561 gccaagctcg gcatcgtcgc cgaggtgctg gacggcgacg aggtgcggct gcggctgccc
     1621 tgggatccga ccaacgtcac gatcgccgac atggtgcacg gcggcgcgat cgccgcactg
     1681 gccgacgtcg cggtgatggc ggccgcgtgg gtgggcgcgg acgtaccgga ctcactgcgc
     1741 ggcgtgacca catcgatgtc gatgcagttt ctggcgccgg cccgcgcgac cgacctgatc
     1801 gcgatcggcc gggtgttgaa gcgcggcaag acattggtga attgcgacgt cgacgtcgtc
     1861 acgcccgagg gtgaagcggt cgcgaaggcg atcgcgacct acaagatcgg ttagagggtc
     1921 acctcgttga gcctgccggt ggcgacgtcg aagatgaaac cgcgcagcga ttcgtgtttg
     1981 gtgacgaacg ggctggcctc gatgcggcgc agcgactgac gcacgtcctc ttcgggatcg
     2041 ggaaacgcct ctgcggccca ttccggtttg ataccggtct catcctggat ggcccgcttg
     2101 aactcgtcgt cggtgaaagt cagcatgccg caatcggtgt ggtggatcag gatgatctct
     2161 ttcgtgccga gcagccgctg gctgatggcc agcgaacgga tctcgtcgtc ggtgatcacg
     2221 ccaccggcgt tgcggatgac gtgcgcctcg ccgtcgccta ggccgagaac ccggtagacg
     2281 tccagccggg cgtccatgca cgccaccacc gcgacgtgct tgctcggtgg cagcggaagt
     2341 gggccggtga acgtgctcgc gtaggcctcg ttgttcttca gatattcgtc ggtcacggac
     2401 attgggacct cctcgatcgc ctacacgcga ccataacaac gcgacggccc cctgccaccg
     2461 gtgaaaatca ctcgaacaga tggcgacggc cgctccgccg ggggtcggcg ttcagcccgg
     2521 aaaccggcga ccgcagcggt gcgatgcgct caacgtgatg tcggggtcgt gatgtttgtc
     2581 ggtgccggcg gtcaggatgg tgaccgtgag taaacgattg cgcgcgatcg gtgaccctgt
     2641 ggtggccgac gcgccggccg gggcgcgcat ccgcacccgg atacatccca cagccgccga
     2701 agccgagacg ctagcggcta tcggcgagct gttggggtcg gtgtatcgca gcgagctagc
     2761 cgagcgggtc cggttgggcc gcttggaccg caaggcccac gcgttgtggc gggcggaacg
     2821 taagcgggcc ctcacggcgg ggtcatcgtc gcggtgggcc ggtgcgatca cccgggctgt
     2881 cgaggaccag taccagctcg ggacgcgcgg cttggccgcc caggtggagg atctacgcgc
     2941 cgccggggag atgctcgagc agaggtgcgc gctgcggccc gacgagctcg cgccggtcga
     3001 gaccaccgac ccccaagctc ggcgccggcg tcggggatac cggaacagcg cggaacggtt
     3061 caccaagacc cgccgcctgg cggtgttgcg tggccggttg gccaccgctg aggaggcgtt
     3121 ggggcgggtc ggccgtcgat gaccgtcggc ggggaaacga ttgtggcgca accgcaacaa
     3181 tctcgacgcc gtcggcatga ccgaacagcg atggcgcgaa aagtgggacg ccgcgcggct
     3241 gtttcttaca gcggatggtg agtcgggcaa agccggcggc aacgaaacca tccgcgtcga
     3301 cgagcacgga caactgcgca tcaaggtgcc cggcgccctg gccgacgaac tcggcaccca
     3361 cctggtgatc gccgcgccgg tgcaattc
//
LOCUS       DASRJA010000196        18465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2116295,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000196 DASRJA010000000
VERSION     DASRJA010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18465
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116295"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="VFC01_08710"
     CDS             complement(<1..138)
                     /locus_tag="VFC01_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZN79752.1"
                     /translation="MTNRIQALLDIEYPVVQAPMTYIARAELAAAVSEGGGLGMIETL
                     TP"
     gene            194..284
                     /locus_tag="VFC01_08715"
     tRNA            194..284
                     /locus_tag="VFC01_08715"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:228..230,aa:Ser,seq:cga)
     gene            complement(445..1074)
                     /locus_tag="VFC01_08720"
     CDS             complement(445..1074)
                     /locus_tag="VFC01_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN79753.1"
                     /translation="MRPVAKTPGAHRVLGLAAAAALAPALVIAPAATPMTLIPAASAA
                     CPDAEVVFARGRQEPPGVGAVGDALVASLRSKVGVSVGVYGVNWPADLSAASGASDIR
                     AHVQSMATSCPNTRMVLGGYSLGATAADLAIRAMPPDMNQHIAAVALFGNGARRLGPA
                     PAFAGKTIDQCADGDPICGRGLNWPSHLQPSYLGSGLVEQAASFIAGKL"
     gene            1454..1636
                     /locus_tag="VFC01_08725"
     CDS             1454..1636
                     /locus_tag="VFC01_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79754.1"
                     /translation="MASRTRFKITFSDGSVEEYEDPMTFDLPGSALKVFNGRERTGPG
                     TVYYSPNAWEKVELIR"
     gene            complement(1680..2363)
                     /locus_tag="VFC01_08730"
     CDS             complement(1680..2363)
                     /locus_tag="VFC01_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006436582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HZN79755.1"
                     /translation="MSQQSRLRRLAHNNGLLLTCLALFAVFFVGMIFSGAATYNQEQQ
                     EHGSKEQVSILHYLTTGHFVEATFENWESEFLQMGMYVVLTAFLYQKGSSESKPIGKR
                     APQDEDPRNITASAETPWPVRRGGWVLAVYEHSLAIAFFALFFASVALHAVGGAKAFN
                     EEQLQHGEAAISTWRYMTTSQFWFESMQNWQSEFIAVAAIIGLSIFLRQRGSAESKPV
                     AEPHHSTGG"
     gene            complement(2375..2551)
                     /locus_tag="VFC01_08735"
     CDS             complement(2375..2551)
                     /locus_tag="VFC01_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79756.1"
                     /translation="MTTDSPASTHATVITDRPDRYGKQLASHLGRLEDVVASRLVRFG
                     EKDELSVVWQRDNG"
     gene            complement(2616..2990)
                     /locus_tag="VFC01_08740"
     CDS             complement(2616..2990)
                     /locus_tag="VFC01_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79757.1"
                     /translation="MKLMAAVALGCGFLAAPMVSATSASAFPVSCDGTDVACVPYVER
                     GAVLGDGCTTSTRYNLGLTASGATLACGAKGQWVASPPLVGTRTLRAQCGEDKGVALS
                     PDGVTLSCVDGAWSADYRVPFA"
     gene            3070..3408
                     /locus_tag="VFC01_08745"
     CDS             3070..3408
                     /locus_tag="VFC01_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN79758.1"
                     /translation="MRWLLGAVIAGTAVLGLAPPANADVVAYLVNVHVRPGYNFPNAD
                     AAIGYGNSICDRVAAKMSYAQLVDQVKADFNTADYYQGAYLINQAVNELCPAQIWQLR
                     QSAAGYTLPA"
     gene            complement(3476..3973)
                     /locus_tag="VFC01_08750"
     CDS             complement(3476..3973)
                     /locus_tag="VFC01_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HZN79759.1"
                     /translation="MKRGLVVAVGGAAIVIAGLSGCSSDEKKSESPATTTAGATTGGG
                     TASATAAPSGGGTKVSIDGQDQNVSGTVVCSAMGGNMNIAIGDAATGIAAVVSEDGSN
                     VQSVGLGNVNGVTLGYQTGTGQGEAKAEKSDKTWKISGTASGIDMANPMQPVNKPFEI
                     EVTCP"
     gene            4159..5064
                     /locus_tag="VFC01_08755"
     CDS             4159..5064
                     /locus_tag="VFC01_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HZN79760.1"
                     /translation="MQSLRTPDEHFADLPEFPYPAKYCEVSDQDGGTLRIAWVEDGPA
                     EANPVLMLHGEPSWSFLYRHMMPILAAAGHRVICPDLVGFGRSDKPTRIEDHTYARHV
                     EWMRALAFDVLDLQQVTLVGQDWGGLIGLRLAAENTDRFARIVVANTGLPTGDLPMPE
                     IWWMFRRAIQSAPHLEVGRFVESGCVRPMTDAVRAAYDAPFPDDSYCAAPRAMPGLVP
                     TAPDDPASEANRAAWKALCASTTPMLVAFSDSDPITGPMAGIFASQMRGAQGVDHPVI
                     ANAGHFLQEDAGEELAEAIVQFLAR"
     gene            complement(5061..5495)
                     /locus_tag="VFC01_08760"
     CDS             complement(5061..5495)
                     /locus_tag="VFC01_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HZN79761.1"
                     /translation="MRTRWAVAAAALLAVAGCSSSPPDYKPAPGTLIAGTAQVTVNGQ
                     NTGTTQAVQCTSTGSLTTISTGDQTSGVTALVSNRDELTAASVGINNVAGFTGSYNAG
                     LGGTAKVTMKGRTYDISGTADGFDTANPSFRTSGTFAVKVAC"
     gene            5513..6745
                     /gene="tgt"
                     /locus_tag="VFC01_08765"
     CDS             5513..6745
                     /gene="tgt"
                     /locus_tag="VFC01_08765"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZN79762.1"
                     /translation="MRESFFTVDAELPGRLGRAGVIRTAHGDIHTPAFIAVGTQATVK
                     AVLPETMKELGAQAVLANAYHLYLQPGPDIVEEAGGLGAFMNWPGPTFTDSGGFQVLS
                     LGAGFKKVLAMDTERVQADDVIAEGKERLAHVDDDGVTFRSHLDGSTHRFTPEMSIGI
                     QHKLGADIIFAFDELTTLVNTRGYQEESVQRTHDWAVRCLVEHRRLTALHPDKPPQAL
                     FGVVQGAQYEDLRRQATRGLTTIVDETGRGFDGYGIGGALEKQNLATIVGWVSSELPA
                     DKPRHLLGISEPDDLFDAVAAGADTFDCVSPSRVARNAAVYSSTGRFNITNARYRRDF
                     SPVDADCDCYTCAHYTRAYLHHLFKAKEILSATLCTIHNERFVIRLVDQIRAAIRAGE
                     FDELRAHVLGRYYSTRAQ"
     gene            6750..7973
                     /locus_tag="VFC01_08770"
     CDS             6750..7973
                     /locus_tag="VFC01_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN79763.1"
                     /translation="MTTAADSQALLLHLLDPSNRADPYPTYTQIRERGPLQLPGMTLN
                     VFSSFQDCDDVLRHPSSASDRLKSTAAQREIAEGAEARPVGPPGFLFLDPPDHTRLRK
                     LVSKAFVPKVVKALEPDITALVDGLLDKVEEQGSFDVIADLAYPLPVAVICRLLGVPI
                     EDEPKFSHASALLAAALDPVISFTGQAPDSFDEMFQAGLWLREYLRDLIALRRSDPSD
                     DLMSALIHVEESGDQLTEEEVVATCNLLLVAGHETTVNLIANAILAMLRHPSQWAALS
                     ADPQRVSVVVEETLRHDPPVQLMGRIAADDMTIGETLVPKGDVMMLLLAAAHHDPAAF
                     DRPDEFDPDRENIRHLGFGKGPHFCLGAPLARLEAAVALSKVTARFPQARMTGEPQYK
                     PNLTLRGLASLEVAV"
     gene            complement(7970..8254)
                     /locus_tag="VFC01_08775"
     CDS             complement(7970..8254)
                     /locus_tag="VFC01_08775"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZN79764.1"
                     /translation="MAILHIEHEISDLATWLETFKRFAPAREQAGVQQAEVFQPADDP
                     NYIVVNLRFESADAASNFRKFLIDVVWASPDISPALVGAPTARVLTEVSV"
     gene            complement(8281..9653)
                     /locus_tag="VFC01_08780"
                     /pseudo
     CDS             complement(8281..9653)
                     /locus_tag="VFC01_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            9748..10953
                     /locus_tag="VFC01_08785"
     CDS             9748..10953
                     /locus_tag="VFC01_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZN79765.1"
                     /translation="MRILLVGAGGVGSAFCAIAARRDFFEQIVVSDYDESRAKQAADA
                     VGDKRFSAARVDATWADDVAAVVSQHGITHVMNAVDPRFVMPIFNGAFAGGADYLDMA
                     MSLSQRHTERPYELTGVKLGDEQFAVEEQWKAAGRLALVGIGVEPGLSDVFARYAADH
                     LFSDIDELGTRDGSNLTVDGYEFAPSFSIWTTIEECLNPPVIWEADRGWFTTEPFSEP
                     EVFDFPDGIGPVECVNVEHEEVLLMPRWVTCRRATFKYGLGTEFIDVLKTLHKLGLDR
                     TEKVNVGGAEVSPRDVVAACLPNPATLGPKMHGKTCAGLWVTGTGKDGNPRSTYLYHV
                     VDNQWSMAEYGHQCVVWQTAINPVVALELLANGTWSGSGVLGPEAFDSVPFLELLTAY
                     GSPWGQKEL"
     gene            10953..12275
                     /locus_tag="VFC01_08790"
     CDS             10953..12275
                     /locus_tag="VFC01_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017207032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HZN79766.1"
                     /translation="MPEDLTAEQAAGRLNAADTLGMPLGPGQPPAFLRALGEREDWTD
                     LRVYGALLAVLTELFTRPGVHYLSGFYGPLDRALRDMGANVDFAPADFRRFGPLLERQ
                     APRVMTTVATPPDADGWCSLSLHAGGTIDELRRAGADPERLLVVEVSDAYPRTLGFGE
                     QYRHALHVDEIDILVRSTDAPLALPGGGAPPTDVDRAIARHAVAFIPSGATLQTGIGG
                     IPNQIAALLAEGDSGDYGLHSEMFTDGCMLLHRAGKVTNTGKGQYGGVSVTTFAFGSA
                     ELYEWLDDNSDVAFLPVEIVNAPDVIGANNDMVSINGALAIDIHGQVVADTISGGQFS
                     GIGGAEDFVAGAGLELSDRSLICLPSTFEKDGELQSRIVPWFGPGAVITTPRHQVDVI
                     ITEYGVAELEGKTVRERGEALAAIAHPQFRDELLAAAERASKGRSPVA"
     gene            complement(12351..13115)
                     /locus_tag="VFC01_08795"
     CDS             complement(12351..13115)
                     /locus_tag="VFC01_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZN79767.1"
                     /translation="MTEPREDRIVAEGVHKAFGDNEVLKGVSFTVAQGSATTIIGPSG
                     SGKTTLLRALNALDVPDAGVIRVGEVELDFSKPVAKDQLRRYRAQSGFVFQSHNLFPH
                     KTVLQNVTEGPIFVQKRPKEEAESDALELLRQVGLAEKRDQYPFQLSGGQQQRVGIAR
                     ALALKPKVVLFDEPTSALDPELVGEVLSVIRDLAVEGWTLVIVTHEIQFARQVSDQVL
                     FTDHGVILEKGPPADVIGNPKEERTRQFLDRILNPL"
     gene            complement(13112..14587)
                     /locus_tag="VFC01_08800"
     CDS             complement(13112..14587)
                     /locus_tag="VFC01_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473011.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZN79768.1"
                     /translation="MHYLRRAPVAVLLLAALVLTACGSSGSNGDNPVKSAGVLRVGTE
                     GVYSPFSYHDPATGQLVGYDVDVARAVADKLGVKVEFVETPWDSIFAALEANRFDVVA
                     NEVTINSERKAKYDLSEPYSVGEGVIVTRANDNSIKTLDDLKRKVAAESATSNWADVA
                     RKAGARVETVEGFTQAIKLLNQGRVDATINDSIAVYAYLAETGDTTVKIAGNVGEKSE
                     QGFAARKDSGLLPDLNKALDELRSDGTLASISQKYLKANASGAPESGQAQHAPRSTWQ
                     LILDSLGPLAKAAITKTVPLTVISFVIGLVIALAVALARLSSNVVLTNVARFYISVIR
                     GTPLLVQLFIVFYAMPELGVKIDPFPAAIIAFSLNVGGYAAEIIRSAIQSIPKGQWEA
                     AETIGMTYVGSLRRIVLPQATRVAVPPLSNTLISLVKDTSLASTILVTELLRQAQIVA
                     APTFEFFALYGTAAVYYWVICLVLSFGQSRIERRLERYVAR"
     gene            complement(14632..15507)
                     /gene="gluQRS"
                     /locus_tag="VFC01_08805"
     CDS             complement(14632..15507)
                     /gene="gluQRS"
                     /locus_tag="VFC01_08805"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HZN79769.1"
                     /translation="MAPGAGRFAPSPSADLHIGNLRTAVLAWLFARSTGRRFVMRVDD
                     LDDRTHADIARRQLDDLSAIGVTWDGVEWQSEHPQRYDAVVDQLADRGLLYECYCSRR
                     DIAQAPRAPHAPQGAYPGTCRDLTDAEREARRRETGRPPALRLRTDAIVYTVHDLLHG
                     EYTGIVDDFVVRRGDGVAAYNLAVVVDDATQGIDQVVRGDDLLPSSPRQAYLARLLGY
                     PGPMYAHVALVLNEDGARLAKRDGAVTLAEIGVQRALAQIASSLGYAAVTPEQMLTQF
                     KPDALPRHPWIYLPV"
     gene            complement(15536..16315)
                     /gene="fabG"
                     /locus_tag="VFC01_08810"
     CDS             complement(15536..16315)
                     /gene="fabG"
                     /locus_tag="VFC01_08810"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZN79770.1"
                     /translation="MFNSLQGRSAIVTGGSKGIGRGIAETFAAVGVNVLVTARNEADL
                     EATASALAGQPGKVSGLSADVSNPEDCRRVVETAVDRHGGLDIVCANAGIFPSGRLEE
                     LTPEDIEQVIAVNFKGTVFIVQAALSALTASGRGRVVITSSITGPITGYPGWSHYGAS
                     KAAQLGFLRTAAMELAPKQITINAVLPGNIITEGLVEMGQEYMDQMASAIPAGRLGSV
                     ADIGNAALFFATDEASYITGQTLVVDGGQILPESHMAIAEL"
     gene            complement(16331..17335)
                     /locus_tag="VFC01_08815"
     CDS             complement(16331..17335)
                     /locus_tag="VFC01_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZN79771.1"
                     /translation="MSDSASTVVSADVHVAERALIEYHIAPESTLNLLNLSENATYAV
                     QDGATGTRSILRVHRQNYHRAHEIESELDWLDALRRDSDIKVPTILPASDGRRVVTVN
                     VDGTPRHVVHFELVAGIEPDEAALTVDDFHMLGSITAALHEHSHNWKRPAGFGRFSWD
                     WPHCLGDEPRWGRWQDADGVGKHEHVLLDRATQLLRQRLFDYGSGPDRFGLIHADLRI
                     ANLLVDGSTITVIDFDDCGFGWFFYDFSTAVSFIEDDPALPEWQESWLRGYRSRRSID
                     AEDEDMLASFVLLRRLLLLAWMGTHSHSKESRTKAITYAEGSCTLAERYLNSNGHRLV
                     "
     gene            complement(17356..17462)
                     /locus_tag="VFC01_08820"
                     /pseudo
     CDS             complement(17356..17462)
                     /locus_tag="VFC01_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886585.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein"
     gene            complement(17484..17849)
                     /locus_tag="VFC01_08825"
     CDS             complement(17484..17849)
                     /locus_tag="VFC01_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79772.1"
                     /translation="MSVTAFQDLPLADRDREWDGDAADKRVRKWAGAEDEPNQKYRDA
                     HVWYDGDKKDNFTAYKLLIADVVGGKLEAVPRGVMAAGNIMEGARGGVDLPKDDIDRV
                     KSHLAKYYKKMGETAPWER"
     gene            complement(17879..>18465)
                     /locus_tag="VFC01_08830"
     CDS             complement(17879..>18465)
                     /locus_tag="VFC01_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN79773.1"
                     /translation="HGGVFIADEVQPGFGRTGEAMWGFDRHGVVPDIVTLAKPMGNGM
                     PVAAMAARDHVLWPFAARVPYFNTYAGNPVSMAAASAVLDVIETDQLQAHAATVGAAL
                     RTELSRIAKDHPRIGDVRGTGLYIGVDVVSDADAKTPDRAGAKALVNAMRERRVLISV
                     CGHDGNVLKIRPPLVFSLTDVDWFCSEFTAALAV"
BASE COUNT         3123 a         6095 c         5963 g         3284 t
ORIGIN      
        1 tggggtaagc gtctcgatca tgccgaggcc gccgccttcg gagaccgcgg cggccagttc
       61 agccctggcg atgtaggtca tcggtgcctg gacgacgggg tattcgatat cgagcagggc
      121 ttggatgcgg ttggtcaccg cctgattgtc ggcgattgga ttcgcgccgc gctcggccgg
      181 taagctgcca cgcggtggcg tgtccgagcg gcctaaggag cacgcctcga aagcgtgtga
      241 cgggtaaccc ccgtccgagg gttcaaatcc ctccgccacc gccaaatctc taccgctgtc
      301 actgcaggtc ggaccctcat ttcatgcccc ggccacgccg actgggcacc gtttgggcac
      361 agaatgggca cacttcaaat caagagatga agcgacggca tctatgcgaa agagtcgctt
      421 gtcgctggcc cggctctggc ccggctagag cttgcccgcg atgaagctgg cggcctgctc
      481 cactagtccg gagccgaggt atgaaggctg cagatgcgac ggccaattca agcctctccc
      541 acagattggg tcgccgtcag cgcactggtc gatcgtctta ccggcaaacg cgggggccgg
      601 gccgagtcgc ctggcgccgt taccgaacaa cgcaaccgct gcgatgtgct gattcatgtc
      661 cggcggcatg gccctgattg ccagatcagc cgccgtggca cccagggaat agccgccgag
      721 caccatgcgg gtgttcgggc aacttgtcgc catggattga acgtgggccc tgatatcgct
      781 ggcgccgctg gcggcgctta agtcggcggg ccagttgact ccatacaccc cgaccgacac
      841 acccaccttg gagcgcagcg aagcgacgag tgcatcgccg actgcgccga cgccgggtgg
      901 ttcctgtcgg ccgcgagcga aaaccacttc ggcatcaggg catgccgccg atgccgccgg
      961 aatcagcgtc atgggcgtcg ctgccggggc aatcaccaat gcaggggcga gcgcggcggc
     1021 ggccgccaga ccgaggacac ggtgcgcgcc tggtgttttc gcgacgggcc tcatgacccg
     1081 atatcggatt acttgtggcg tacgttacgt cgcgcgcgag gctgcggcgt aggctcgctc
     1141 gggctggttc cgccgcaccc ggcgaccaac gaatcggtgc tgtaacgagt ttggggcaaa
     1201 cccggataga cgcgccgtgg atctgatttg gtgtcgatcg cggtgtgtcc ccgtgcgcct
     1261 cggcgaagct gcgagaccag ggcgtgaatc gttcgacgcc gccgatgatg agttaggtcg
     1321 gctcatccgt gagggattcg agcaagggat cgccggccga tcagccagcc gaatccctcc
     1381 gccaccgcca acgccttccg acgggaagcc ttgaccggtg ccctcgggct gaacctcatg
     1441 ccacactccc agggtggcta gtagaacccg tttcaagatc accttctcgg acggcagcgt
     1501 cgaagagtac gaagacccca tgaccttcga tctccccgga tccgccctca aggtcttcaa
     1561 cggcagagag agaaccggcc ccggaacggt ctactactcg ccgaacgcct gggaaaaggt
     1621 cgaattgatc cgctgatccg ccctcgcggg tgaccattcc ttcgcccaga gcgcgcggct
     1681 caaccgccag tgctgtggtg cggctcggcc accggctttg attcggcaga cccacgttgc
     1741 cggaggaaga tcgacaagcc gatgatcgcg gccacagcga tgaactcgct ctgccagttc
     1801 tgcatcgatt cgaaccagaa ttgcgatgtg gtcatatacc gccatgtcga gatggcggcc
     1861 tcgccgtgct gaagctgttc ttcattgaac gctttcgcgc cgccgacagc gtgtagcgct
     1921 actgacgcga agaacaaggc gaagaacgca atcgccagag aatgctcgta gaccgccagc
     1981 acccacccgc ccctgcggac cggccacgga gtctcggccg aggccgttat gttgcgcggg
     2041 tcttcgtcct gcggtgcgcg cttgccgatc ggcttcgatt cagaggaacc cttctgatac
     2101 aggaatgccg tcaacacgac gtacatcccc atctgcagaa actccgactc ccagttctcg
     2161 aacgtggctt cgacgaagtg gcccgtcgtg aggtagtgca atatcgacac ttgctctttc
     2221 gagccgtgct cttgctgctc ctggttgtag gtcgccgcgc cggagaaaat cataccgacg
     2281 aagaagaccg cgaacagcgc gaggcaggtc aagagcaaac cgttgttgtg ggccagccgc
     2341 cgtaatcgac tttgttgcga catgcgcggt cggttcagcc gttgtcgcgt tgccacacaa
     2401 cggatagctc gtccttttcg ccgaatcgga cgagacggct ggccaccacg tcctcgaggc
     2461 ggcccaggtg tgacgccagt tgcttgccgt accggtcggg ccggtcggtg ataacggtcg
     2521 cgtgtgtgga ggccggactg tccgtcgtca tcgcatctcc tttcactgcg gggtgtaccc
     2581 agcgcgacgt gaaagacgag atgttcggac cgtccctagg cgaacggcac cctgtagtcg
     2641 gctgaccacg cgccgtcaac gcagctcaac gtcacaccgt cgggtgacag cgccacgccc
     2701 ttgtcctcgc cgcattgcgc gcgcagcgtg cgagtgccga ccagcggcgg cgacgcgacc
     2761 cattgcccct tggcgccgca ggccaacgtc gcgcccgatg ccgtcaggcc caggttgtat
     2821 cgggtgctcg tggtgcagcc gtcgcccagg acggcgccgc gctcgacata cggaacacac
     2881 gccacgtcgg tgccgtcgca cgacaccgga aacgccgacg cgcttgtcgc cgacaccatc
     2941 ggtgccgcga gaaatccaca acccaacgcc actgcagcca tgagcttcat ggcgcttagc
     3001 gtatgtgcct cgccgtgggg ttggctcaac aatcgccaca gtcgatgccg cgcactacgc
     3061 tcatgtgcca tgagatggct gctcggcgcg gtgatagcag gcacggcggt ccttggattg
     3121 gcgccgcccg ccaacgccga cgtggtggca tatctggtca acgtgcacgt gcggccgggc
     3181 tacaacttcc ccaacgccga cgccgcgatc ggctacggca acagcatctg cgatcgggtc
     3241 gccgcgaaga tgagctacgc gcagctggtc gaccaggtca aggccgactt caacacggcc
     3301 gactactacc agggcgccta cctgatcaac caggcggtca atgagctgtg ccccgcgcag
     3361 atctggcaac tgcgccagtc ggcggccggc tacaccttgc ctgcctgacc tcgcgcacga
     3421 caacggcggc gcccctgccg ggacgccgcc atcgtttcga actaggagta cctggctacg
     3481 ggcaggtgac ctcgatttcg aacggtttgt tcaccggctg catggggttg gccatgtcga
     3541 taccgctcgc ggtgccgctg atcttccagg tcttgtcgct cttttcggcc ttggcctcgc
     3601 cctggccggt gcccgtctgg taacccagcg tgacgccgtt gacgttgccg agcccgacgg
     3661 actgcacgtt agagccgtcc tcgctgacca ccgccgcgat accggtcgcc gcgtcgccga
     3721 tcgcgatgtt catgtttccg cccatcgcgc tgcacacgac ggtgccagaa acgttctggt
     3781 cctggccgtc gatggagacc ttggttccgc cgccgctggg tgcagctgtg gcgctggcgg
     3841 tccccccgcc agtggttgcc cctgccgttg tagttgccgg actctcggac ttcttctcgt
     3901 ccgacgaaca gcctgacaga ccggcgatga caatcgccgc gccgccgacg gcgaccacca
     3961 aaccacgttt caccactgtt ctcctttgtg tttgtcgtga cccgtcaaga tcggcggatc
     4021 atcccggaca gtatggtggg ccctttcaac gcggatatgg cgattgggcg aaaaatttgc
     4081 ctgtgcaccg gctgttcgtt atctgcagtt gatgcgaacg gcggtatcga aatcgggata
     4141 ttcggtatgt tcgcggtcat gcagagtttg cgcacgcccg acgaacattt tgccgatctg
     4201 cctgaattcc cttatcccgc aaagtattgc gaggtttccg accaggacgg cggtactctg
     4261 cggatcgcat gggtggaaga tggtcccgcc gaagcgaatc cggtgctgat gctgcacggc
     4321 gagccgtcgt ggtcatttct gtaccgccac atgatgccga ttttggcggc ggccggccat
     4381 cgcgtcattt gtccggacct ggtcggcttc ggccggtccg ataagccgac ccggatcgag
     4441 gaccatacct acgcccgtca cgtggagtgg atgcgagcgc tggcgttcga cgtgctcgac
     4501 ctgcagcaag tgacgctcgt cggccaggac tggggcggcc tgatcgggct tcggctcgcc
     4561 gcggaaaata cggatcgctt cgcccgcatc gtcgtcgcca acaccggcct gcccaccggc
     4621 gatctgccga tgcccgagat ctggtggatg ttccgcaggg ccatccaaag tgcgccgcat
     4681 ctcgaagtcg gcaggtttgt cgagtccggt tgcgttcgtc cgatgaccga cgccgtccgc
     4741 gccgcttacg acgcgccatt ccctgacgac tcctactgcg cagcacctcg cgctatgcct
     4801 ggtctggtcc ccaccgcgcc tgacgacccg gcatcggagg ccaaccgtgc cgcgtggaaa
     4861 gcgctgtgcg ccagcacaac cccgatgctc gtcgcgttca gcgacagcga cccgatcacc
     4921 gggccgatgg cggggatctt cgccagccag atgcgcggcg cgcagggcgt cgaccatccc
     4981 gtcatagcga acgccgggca cttcttacag gaggacgcgg gcgaggaact cgccgaggcg
     5041 atcgtgcagt tcttggcgcg ctaacacgcc accttgacgg cgaaggtacc ggaggtgcga
     5101 aagctgggat tggcggtgtc gaaaccgtcg gcggttccgg agatgtcgta ggtccggccc
     5161 ttcatcgtga ccttcgcggt gcccccgagg cccgcgttgt aactgccggt gaagccggcg
     5221 acgttgttga tgccgacgga tgcggcggtg agctcgtccc tgttggagac cagggccgtg
     5281 acgcccgatg tctgatcgcc ggtgctgatc gtggtcagtg aacccgttga cgtgcactgc
     5341 accgcctggg tggtgccggt gttctgcccg ttgacggtca cttgcgcagt gcccgcgatg
     5401 agcgtgcccg gcgccggctt gtagtccggc gggctggagg aacaccccgc gacagccagc
     5461 aatgccgcgg cggcgacggc ccatcgagtt ctcacagtgc agaatctacg gcgtgcgcga
     5521 gtcgttcttc accgttgacg ccgaattgcc cggccggctc ggtcgcgccg gcgtcatccg
     5581 caccgcgcac ggcgacatcc acacacccgc gttcatcgcc gtcggcaccc aggccaccgt
     5641 caaggccgtg ctgcccgaaa ccatgaaaga gcttggcgca caagctgttc tggccaacgc
     5701 ctaccacctg tatctgcaac cgggccctga catcgtcgag gaggcaggcg ggctcggcgc
     5761 gttcatgaat tggcccgggc cgacgttcac ggacagcggc ggatttcagg tgctctcact
     5821 cggtgccgga ttcaagaaag tcctggcgat ggacaccgaa cgggtccagg ccgacgacgt
     5881 catcgccgag ggcaaggagc ggctggccca tgtcgatgac gacggtgtca ccttccggtc
     5941 gcatctggac ggctccaccc atcgcttcac tcctgagatg tcgatcggga tccagcacaa
     6001 gctcggcgcg gacatcatct tcgccttcga tgagctgacc acgctcgtca acacccgcgg
     6061 ctaccaggaa gagtcggtgc agcgcaccca cgactgggcg gtgcgatgcc tggtcgaaca
     6121 ccgccgcctg accgccctgc atccggacaa gccaccgcag gcgttgttcg gcgtggtgca
     6181 aggcgcccaa tatgaggatc tgcgcaggca ggccacgcgc gggctgacca cgatcgtcga
     6241 cgaaaccggc cgtgggttcg atggctacgg catcggcggg gcgctggaaa agcagaacct
     6301 cgcgaccatc gtcggctggg tcagcagcga gctgcccgcg gacaagcctc gtcacctgct
     6361 cggcatcagc gagcccgatg acttgttcga cgcggtcgcc gccggcgccg acacgttcga
     6421 ctgcgtgtct ccgtcgcggg tggcacgcaa cgccgcggtc tattcctcga cagggcgctt
     6481 caacatcacc aacgcccggt atcgacgtga cttcagtccc gtcgacgccg actgcgactg
     6541 ctacacgtgc gcgcactaca cccgcgccta cctgcaccac ctgttcaagg cgaaggagat
     6601 cctgtcggcg acgctgtgca cgatccacaa cgagcgcttc gttatcaggc tcgtcgacca
     6661 gatccgcgcg gcgatccgtg ccggcgagtt cgacgaactc cgcgcgcacg ttctgggccg
     6721 gtattactcg acgcgtgcac aataagtaga tgacgaccgc cgcggattcg caggcattgc
     6781 tgctgcatct gctcgacccg tcgaaccggg cagaccccta tcccacgtac acgcagatcc
     6841 gggagcgcgg gcccctgcag ctgccgggca tgacgttgaa tgtgttctcg tcatttcagg
     6901 actgcgatga cgtgctgcgg catccgtcat cggccagtga tcgactgaag tcgactgcgg
     6961 cgcagcgcga gatcgccgag ggcgccgagg ctcgcccggt aggtccgccg gggttcttgt
     7021 tcctcgatcc gccggaccac acccggctgc gcaagctggt cagcaaggcg ttcgtgccga
     7081 aggtggtcaa ggcgctggag cccgacatca cggcactggt cgacgggttg ctcgacaagg
     7141 tcgaggagca aggctccttt gacgtgatcg ccgacctggc ctatccgctg ccggtcgcgg
     7201 tgatctgccg gctgctcggc gtcccgatcg aggatgagcc gaaattcagc cacgcatcgg
     7261 cgctgctcgc ggcggcactg gacccggtca tctcgttcac cggtcaagcg cccgacagtt
     7321 tcgacgagat gtttcaggcc gggctgtggc tgcgcgagta cctgcgagac ctgatcgcac
     7381 ttcggcgcag cgaccccagc gacgatctga tgtcggcgtt gatccatgtc gaggagtccg
     7441 gggatcagct caccgaggag gaggtcgtcg cgacgtgcaa cctgctgctg gtcgccggcc
     7501 acgagacgac ggtgaacctg atcgctaatg cgatcctggc gatgttgcgc cacccgtcgc
     7561 agtgggcggc gctcagcgca gacccgcagc gggtgtccgt ggtggtggag gagacgctgc
     7621 gccacgaccc gcccgtacag ctgatgggcc gaattgccgc tgacgacatg acaattggcg
     7681 agactttggt gcccaagggc gacgtgatga tgctgcttct tgccgcggcg caccacgacc
     7741 cagccgcgtt cgatcggccc gacgaattcg accctgaccg cgaaaacatt cgtcacttgg
     7801 ggttcggcaa gggtccgcac ttctgcctcg gcgctccgtt agcccgcttg gaggctgccg
     7861 ttgcgctgtc gaaggtgacg gcacgctttc ctcaggcgcg gatgacgggc gagccgcagt
     7921 acaagccgaa cctgacattg cgcgggctag cctcgcttga ggtcgcggtc tagacgctca
     7981 cctcggtcag gactctggcc gtcggcgcgc cgacgagtgc gggggagatg tccggcgacg
     8041 cccacaccac gtcgatgagg aacttgcgga aattgcttgc ggcgtcagcg ctttcgaatc
     8101 gcagattgac cacgatgtag ttggggtcgt cggctggctg gaacacctcg gcctgctgga
     8161 ctccggcctg ctcacgcgcg ggcgcgaacc gcttgaacgt ctcgagccac gtcgcaagat
     8221 cactgatctc gtgttcgatg tgcagtatgg ccatggctca gtgtggaccg cggatggggg
     8281 ctaccgcacc aattcggcga tggttgcctc cgcggcccgc tccccggcat cgaccgcgcc
     8341 ctccatgtat gcgctccagt cggtcgcggt atcggttgac gcccagtgga tcacgccgat
     8401 aggcgtggtg agcgcagggc cgtaggtcgt ccacacatgt ggtccgtggt tggcgttgta
     8461 gcagccgcgg gtccattgcc ggtccgacca ttcgccgtcg acgtagaact cgggtttggc
     8521 tgccgcgcca ccgaagtggc gtaccagttc tgcggtgaga gcttcgcggc ggtcggcctc
     8581 ggggatgcgg ccgtaggtgc gggcctgctc gccttctaag aagagcagga tcacgccgtg
     8641 gtcgttgccg gggatacagg tgtcgttgga cattcgtgcc ggccgacgtc ggagatgagt
     8701 tggccgttga atccgtcggc acgccagaat ggttcgtcgt agatgaagaa cgccttcatg
     8761 gccgagccgt tgggcagtcg ctgggtgagc tgatcgcgga tgccgggaag aggcgggtcg
     8821 tacatgattc gtccggcgag tgtcggcgat aatgcgacga tgactcggcg gccgcgggcc
     8881 accagcccgc cgcggcagta cacggtgacg ctgtcggcgg tgtgttcgat gagctggacc
     8941 ggcacgttga gcacgatgtg gtcggtgatc aacgcggcca gtcgacgcgg gatctcgccg
     9001 gtgcctccga tgaaccgcgc ggtctgcgcg ccgccctccg attcggcgaa cagctccgat
     9061 gtcaccccgc aggtctggat ggtgaacagc aggtgcagga aagacacttc ggccgtgggc
     9121 acggcgagga ttccgaccgt gcagatctcc aacagggtgc gggccaccgg cgaaagccct
     9181 tgcgcgtcat accaggtgcc cgcggtaatg gcatcccact cggcggcccg tggcgccaac
     9241 cagggcgctt cgaccggaac ggcggtcgcc agcacatcga gccggcgcag caccagctcg
     9301 agttccttca actccggagc gaaccgacgg tggaactcgt tctcccgcag cacacctgag
     9361 ccgaccagct cgtaggacgt ttcgccttcg tcgtactggg gaaaggtctc gacaccgagt
     9421 tcggctgcca atcggaacat ccgctcgtgc gtgtcaccga tccactgggc cccgagctcg
     9481 acgggaatgc cgggcagcac ctcctgcgtg aggatgcgcc cgccgacgcg ctcgtcggcc
     9541 tccaggacca gcggcgtgag ccctgcgtcg agcaccttgc gcgcggcgat catccccgaa
     9601 agccccgctc caacgatgat gacgtccgct tccaggttcg acggctgtgt catggtgcaa
     9661 ccctctcacc gcttgcacgc gcccacttcg ctttcagtcg caacagaccg actctggttc
     9721 ggaatccttg cctgtacggt cgagcgcatg cggatcctat tagtgggcgc cggcggcgtc
     9781 ggttcggcgt tctgcgccat cgcggcacga cgcgacttct tcgagcagat cgtcgtcagc
     9841 gactacgacg agtcacgcgc caagcaggcg gcggatgcag tcggcgacaa gcgcttctcc
     9901 gccgcgcgcg tcgacgcgac atgggccgac gacgtcgccg cggtggttag tcaacatggc
     9961 atcacccacg tgatgaacgc cgtcgacccg cggttcgtga tgccaatctt caacggcgcg
    10021 ttcgccggtg gcgccgatta cctcgacatg gcgatgagcc tctcgcaacg ccataccgag
    10081 cggccctacg aactcaccgg agtcaagctc ggcgacgagc agttcgccgt cgaggagcag
    10141 tggaaggccg ccggccggct tgcgctggtc ggaatcgggg tggagccagg cctttccgac
    10201 gtgttcgcac gctacgccgc tgaccatctg ttctccgaca tcgacgagtt gggcacccgc
    10261 gacggatcca acctcaccgt cgacggctac gagttcgcgc cgtcgttctc gatctggacc
    10321 accatcgagg agtgcctcaa cccgccggtg atctgggaag ccgaccgcgg ctggttcacc
    10381 accgaaccgt tttctgagcc cgaggttttc gactttcccg atggcatcgg cccggtcgag
    10441 tgcgtcaacg tcgagcacga agaagtgctg ctgatgcccc gctgggtcac atgcagacgc
    10501 gccacgttca agtacggcct cggcacggag ttcatcgacg tgctcaagac gctgcacaag
    10561 ttgggcctcg accgcaccga gaaagtcaac gtcggtggcg cagaggtcag cccacgcgac
    10621 gtggtcgcgg cctgcctacc gaatcccgcc acgctgggac cgaagatgca cggcaagacg
    10681 tgcgccggcc tgtgggtgac gggcaccggc aaggacggca atccgcgatc gacctacctg
    10741 taccacgtcg tcgacaacca atggtcgatg gccgagtacg gccaccagtg cgtggtgtgg
    10801 cagaccgcga tcaatcccgt tgtggcactc gagctcctgg ccaacggcac gtggagcggc
    10861 agcggcgtac tcggccctga ggcgttcgac tcggtgccgt tcctcgagct gctgaccgcc
    10921 tatggctcac cgtggggcca gaaggagctg tgatgccaga ggacctgacc gccgagcagg
    10981 ccgcagggcg gctgaacgcc gccgacacgc tgggaatgcc gctcggccca ggccagccac
    11041 cggcgttcct gcgcgcgctc ggcgagcgcg aagactggac agatctgcgc gtatacggcg
    11101 cgctgctcgc ggttctcacc gaactgttca cacgcccggg cgttcattac ttgtctggct
    11161 tctatggtcc gctcgaccga gcgctgcggg acatgggcgc caacgtcgac ttcgctcctg
    11221 cggactttcg ccgcttcggg ccgctactcg agcgccaggc gccgcgtgtg atgacgaccg
    11281 tcgcgacacc gcccgatgcg gacggctggt gctccctgtc actgcatgcg ggcggcacaa
    11341 tcgacgaatt gcgccgcgcg ggagccgatc ccgaacggct gctggtcgtc gaggtttcag
    11401 acgcttaccc acggaccctc ggcttcggcg agcagtatcg ccatgcgctg cacgtcgacg
    11461 agatcgacat cttggtccgc tcgaccgacg cgccgctcgc gctacccgga ggcggcgcgc
    11521 cgccgacaga cgtcgaccgg gcgatcgcac ggcacgccgt cgccttcatt ccgtccggcg
    11581 caaccctgca gacggggatc ggcgggattc ccaaccagat cgctgcgctt ctggccgaag
    11641 gagatagcgg cgactatggg ctgcacagcg agatgttcac cgatggctgc atgctgctgc
    11701 accgcgcagg caaggtcacc aataccggca aggggcagta cggcggggtc agcgtgacga
    11761 cgttcgcgtt cggttcggcg gagctctacg agtggctgga cgacaactcc gacgttgcgt
    11821 tcctgccggt ggagatcgtc aacgcaccgg acgtgatcgg cgccaacaac gacatggtct
    11881 cgataaacgg cgcgctggcg atcgacatcc acggccaggt cgtcgccgac acgatctccg
    11941 gcggccagtt cagcgggatc ggcggcgccg aggacttcgt ggccggcgcc gggctcgagc
    12001 tctcggaccg ctcgctgatc tgtctgccct cgacgttcga gaaggacggc gagctgcagt
    12061 cgcggatcgt gccgtggttc ggtcccggcg cggtgatcac gacgccgcgg caccaagtcg
    12121 acgtgatcat caccgagtac ggcgtcgccg aactcgaggg caagaccgtc cgggagcgcg
    12181 gcgaggcgct tgcggcgatc gcacatccgc aattccgcga cgagctgctg gcggccgccg
    12241 agcgcgcgtc gaagggccgc tcacccgtgg cgtaaccgaa gcgcgaacgt gggttaccgt
    12301 cacgccaacg gccgccgaag cgtgtatcaa tcccacactc gtgcgaagaa ctacagcggg
    12361 ttgaggatgc ggtccaggaa ttggcgcgtg cgctcttcct tcgggttgcc gatcacatcg
    12421 gcgggcggac ccttctccag gatgacgccg tggtcggtga acagcacctg atcggaaacc
    12481 tgtcgggcga actggatttc gtgcgtgacg atcaccagcg tccagccctc gacggccaga
    12541 tccctgatca ccgacagcac ctcgccgacg agttcggggt ccagcgccga cgtcggctcg
    12601 tcgaacagca ccaccttcgg cttaagcgcc agtgcccgcg caatgccgac ccgctgctgc
    12661 tgtccgccgg acagctggaa cggatactgg tcacgcttct cggccaggcc gacctgccgc
    12721 agcagctcga gcgcgtcgga ctccgcttcc tccttcgggc gcttctgcac gaagatcggc
    12781 ccctcggtca cgttctgcag caccgtcttg tgcggaaaca ggttgtgcga ctggaacaca
    12841 aagccgctct gcgcgcggta gcgccgcagt tggtccttcg ccaccggctt cgagaagtcc
    12901 agctcgacct cgccgacgcg gatcacgccc gcgtccggca cgtctagcgc gttgagcgcg
    12961 cgcagcagcg tggtcttgcc cgaccccgac gggccgatga tcgtcgtcgc cgagccctgc
    13021 gccacggtaa acgaaacccc tttgagcact tcgttatccc cgaaggcctt gtgcacgccc
    13081 tcggcgacga tcctgtcttc acgcggctct gtcatcgcgc cacatacctt tccaaccgac
    13141 gttcaatgcg gctctggccg aacgagagca ccaggcagat gacccagtag tacaccgcgg
    13201 ccgtgccgta caacgcgaag aactcgaacg tcggcgccgc gacgatctgg gcttgccgca
    13261 gcagctcggt gaccaggatc gtcgacgcca gcgaagtgtc tttcaccagt gagatcaacg
    13321 tgttcgacag tggcggcacc gccacccggg tggcctgcgg aaggacgatg cgccgcaacg
    13381 accccacata ggtcatgccg atggtctcgg ccgcctccca ctggcccttc ggaatgctct
    13441 ggatcgccga gcggatgatc tctgccgcgt agccgcccac gttcagagag aacgcgatga
    13501 tcgcggcggg gaacgggtcg atcttcactc ccagctcggg catcgcgtag aagacgatga
    13561 aaagctgcac cagcaacggt gttccgcgga tgaccgagat gtagaaccgc gcaacgttgg
    13621 tcagaaccac gttcgacgac agccgcgcca gcgccaccgc caaggcaatc accagcccga
    13681 tgacgaagct gatcaccgtc agcggaaccg tcttggtgat cgccgccttg gccagcggcc
    13741 caaggctgtc cagaatcagc tgccaggtcg accgcggcgc atgttgcgcc tggcccgact
    13801 cgggtgcacc ggaggcgttg gccttcaggt acttctgcga gatgctggca agcgtcccgt
    13861 cggagcgcag ctcgtcgaga gccttgttga ggtcgggcag cagcccgctg tccttgcggg
    13921 cggcgaagcc ctgctcgctc ttctccccga cgttcccggc gatcttgact gtcgtgtcac
    13981 cggtctcggc gaggtaggcg tacaccgcga tgctgtcgtt gatggtggcg tccactcgcc
    14041 cttggttcag caacttgatg gcctgggtga agccctccac ggtctcgacg cgagctccgg
    14101 ccttgcgagc gacgtcggcc cagttgctcg tcgcgctttc ggccgcgacc ttgcgcttca
    14161 ggtcgtccag cgtcttgatg gagttgtcgt tggctcgcgt gactatgacg ccctcgccga
    14221 cggagtacgg ctcggaaagg tcgtacttcg ccttccgctc cgaattgatg gtcacctcgt
    14281 tggcgacgac gtcgaagcgg ttggcttcaa gcgcggcgaa gatcgaatcc catggcgtct
    14341 caacgaattc gaccttgacg ccaagcttgt cggccaccgc ccgggctacg tcgacgtcat
    14401 agccgacgag ctggccggtc gcgggatcgt ggtagctgaa cggcgagtac acgccttcgg
    14461 tgccgacccg cagcacgcca gccgatttaa ccgggttgtc gccattgctg cccgacgaac
    14521 cgcacgccgt gagcaccagc gcggcgagta gcaaaacagc cacgggggct cttcgtagat
    14581 agtgcaccgg cggaagctag cagggcgcca ggcgccgccc aagggttctg ctcacacggg
    14641 gaggtatatc cacgggtgcc gcggcagcgc atcgggtttg aactgcgtga gcatctgctc
    14701 gggggtcacg gcggcatagc ccaacgacga cgcgatctgc gcgagcgccc gctggacgcc
    14761 gatttcggca agcgtcaccg cgccgtcccg cttggccagc cgcgccccgt cctcgttgag
    14821 caccagcgcg acgtgtgcgt acatcggccc tggatacccg agcagccgcg cgaggtatgc
    14881 ctgcctcggc gacgacggca gtaggtcgtc tccgcgcacc acctggtcga tgccctgcgt
    14941 ggcgtcgtcg accaccacgg caaggttgta ggccgcgaca ccgtcgccac ggcggaccac
    15001 gaaatcgtcg acgatgccgg tgtactcacc gtggagcagg tcatgcactg tgtagacgat
    15061 cgcatcggtg cgcagccgca gcgcaggcgg ccggccggtc tcgcgccgcc gggcttcgcg
    15121 ctccgcgtcg gtcaggtcgc ggcaagtgcc cggatacgcg ccctgcggcg catgcggggc
    15181 tcgcggtgct tgcgcgatat cccttcggct gcaatagcat tcgtacaaca gtccgcgatc
    15241 agccaactga tccacgacgg cgtcatagcg ctgcggatgt tccgactgcc actcgacgcc
    15301 gtcccacgtc acccctattg cggacagatc gtcgagctgg cggcgggcga tgtcggcgtg
    15361 ggtgcggtcg tcgaggtcgt cgacacgcat cacgaaccgg cgaccggtgg agcgcgcgaa
    15421 cagccacgcc agcacggccg tgcgcaggtt gccgatgtgc agatcagccg acgggctggg
    15481 cgcgaaccgg cctgctccag gtgccatgtc ctcagcttgc cctggtgtgg agggactaga
    15541 gctctgcgat cgccatgtgc gattcgggaa ggatctgacc gccgtcgacg acaagcgtct
    15601 gccctgtgat gtaagaagcc tcgtcagtgg cgaagaacag cgcggcgttg ccgatatcag
    15661 cgacactgcc gagtcggccg gcgggtatcg ccgaagccat ttggtccatg tattcctgac
    15721 ccatttccac caggccttcg gtgatgatgt tgcccggcag cacggcattg atggtgatct
    15781 gcttgggcgc cagctccatc gccgcggtgc gaaggaaacc tagctgggcg gccttgctgg
    15841 caccgtagtg ggaccagccc gggtagccgg tgatcggtcc ggtgatcgac gacgtgatga
    15901 ccacgcggcc gcgtccgctg gcggtcagag ccgacagcgc ggcctgcacg atgaacacgg
    15961 tgcccttgaa gttgaccgcg atgacctgct cgatgtcctc cggtgtcagc tcttccagac
    16021 gtccggacgg gaagatgccc gcattggcgc acacgatgtc gaggccgccg tggcggtcga
    16081 ccgccgtctc caccacccga cgacaatcct cgggattgga cacgtctgcg ctcaggccac
    16141 tgaccttccc gggctgtccg gccagagcgg acgcggtggc ctccagatcg gcttcgtttc
    16201 gcgccgtcac caacacgttg acgcccacgg ctgcaaaggt ttccgcgatc ccgcggccga
    16261 tgcccttgct gccgccggtg acgatggccg agcggccctg tagtgaattg aacatgtgcg
    16321 tgtccttcga tcagaccagt cgatggccgt tggagttgag ataacgctcg gcgagtgtgc
    16381 agctgccctc ggcataggtg atcgccttgg tgcgggattc cttggaatga ctatgtgttc
    16441 ccatccatgc cagcaacagc agccggcgca acagcacgaa cgacgccagc atgtcttcat
    16501 cttcggcgtc gatggaacgg cggctgcggt atccgcgcag ccaggactct tgccattcgg
    16561 gcagcgccgg gtcgtcctcg atgaacgaca ccgcggtgct gaaatcgtag aaaaaccacc
    16621 cgaatccgca gtcatcgaag tcgatgacgg tgatcgtcga gccgtcgacc agaaggttgg
    16681 cgatccgcaa gtcggcgtgg atgagcccga accggtccgg cccggatccg tagtcgaaca
    16741 agcgctggcg cagcagttgc gtcgccctgt ccagcagtac atgctcgtgc ttaccgacgc
    16801 cgtcggcgtc ctgccagcgg ccccaccgcg gctcgtcacc gaggcagtgt ggccaatccc
    16861 aggagaatct accgaacccg gccggtcgct tccagttgtg tgaatgttcg tgcagcgcag
    16921 cggttatcga cccaagcata tggaagtcat cgacggtcaa tgccgcctcg tcgggctcga
    16981 taccggcgac caactcgaaa tgcaccacgt gccgcggtgt tccgtcgacg ttcacggtga
    17041 ccacccgacg accgtcactg gccggcagga tggtgggcac cttgatgtcg ctgtcgcgcc
    17101 gcagcgcatc gagccagtcg agttcggatt cgatctcgtg cgcgcggtga tagttctgcc
    17161 ggtgcacacg caggatcgag cgagtaccgg tcgcgccgtc ctgtaccgcg taggtcgcgt
    17221 tctccgacag gttcagcagg ttcagcgtcg actccggcgc gatgtggtat tcgatgagcg
    17281 cccgctccgc tacgtgcacg tcggcagaga cgaccgtact ggccgagtcg ctgatgaggc
    17341 cgtttgagtt gtccgtcaat ggtcgcgccc ttcgatgccg gccagcacct cggtgatgcg
    17401 gcgccgatca tctgtacgtc taccacggtc agcgcgggtg ccgctcgttc agcctcgttg
    17461 gcgcggtccg ccacgacaca gggttagcgc tcccagggag ccgtctcgcc catcttcttg
    17521 tagtacttgg cgaggtggct cttgacccga tcgatgtcgt ctttgggtag gtcgacgccg
    17581 ccgcgggcac cctccatgat gttgcctgcc gccatcactc cgcggggcac ggcctcgagc
    17641 ttgccgccga caacgtcggc gatcagcagc ttgtaggcgg tgaagttgtc cttcttgtcg
    17701 ccgtcgtacc agacgtgggc atcgcggtac ttctggttgg gctcgtcctc tgcgccggcc
    17761 cacttgcgaa cacgcttgtc cgccgcatcg ccgtcccatt cgcggtcacg atcagcgagc
    17821 ggcagatctt gaaatgctgt cactgacatg tgggaggcgt gcccgcagtg ccagttcgct
    17881 aaaccgccag cgcagctgtg aattcgctgc aaaaccagtc gacgtcggtc agggaaaaca
    17941 ccaggggtgg gcggattttc agcacgttgc cgtcatggcc gcagaccgag atcaggaccc
    18001 gtcgctcgcg catggcgttg accagtgcct tggcgcccgc ccggtcaggt gtcttcgcgt
    18061 cggcgtcgct gacgacgtcg accccgatgt acagcccggt accgcgtacg tcgccgatcc
    18121 gcgggtggtc tttcgctatc cgggacagtt cggtgcgcag cgcagcaccg actgtcgcgg
    18181 catgggcttg tagctggtcg gtctcgatga catcgagcac cgcggatgcg gcggccatcg
    18241 acacggggtt gcccgcgtag gtgttgaaat acggcacccg cgccgcgaac ggccatagca
    18301 cgtggtcacg tgcggccatc gcggccaccg gcatgccgtt acccatcggt ttggccaacg
    18361 tgacgatgtc gggaaccacg ccgtggcggt cgaagcccca catggcttcg ccggtgcggc
    18421 cgaacccggg ctgcacctca tcggcgatga atacaccgcc gtgcc
//
LOCUS       DASRJA010000197        15329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_150391,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000197 DASRJA010000000
VERSION     DASRJA010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15329
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150391"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VFC01_08835"
     CDS             complement(<1..1175)
                     /locus_tag="VFC01_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79774.1"
                     /translation="MTTTFERQSVTSTTQGGLNDRSLGWLRFLWDKATTDDDWSDQGE
                     PHQWWDRYSEPPMCSFPRFDLAMMGYVLPMMLEATPAWREGYTRILDEIARRYVTFWG
                     AIDWNTLIGPDPGVDKYPPEWLMVAPEHLRGRYSLPGWTGNGIEPWGLQPDPVGCDGN
                     LFYRGWLNLLLSMRHYVSGQPTQHVTFDVSGYQNRQFSWTHQRIAQFISDQLTARPQG
                     AHCENTKIWPFCMSAAGLGLKLYDSLLHTSLNEPFLDWIAFAKKHYMGRDRRGRIDWV
                     ALYYDPIENATMAFPGPLNAYSALCLLHYLYPQDPQLAIDLYESATRQLGWSNPKVPV
                     VQLADDPQMLSNGLWMAREIGDTTTWQRLRAVSESQFEPTFFGQDNSRFAYWLDRGEP
                     "
     gene            complement(1177..1743)
                     /locus_tag="VFC01_08840"
     CDS             complement(1177..1743)
                     /locus_tag="VFC01_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79775.1"
                     /translation="MADAALRTLLWLLGQCIARGSRYMPSLRSQITRTMTFELSAGER
                     VARHWVFDAQLRRATTYSGHASAPDCAVHINSSWQALRALVSPVTVDKIVRGLHEGTV
                     ELHGSAFVLLWFHGLTRKFVKLGRPDGPRHRIPGTYIAHDPAACGAETIVIEPAVVRL
                     DPTWTGAWKARSTLSTVRACTDEPMLEP"
     gene            complement(1764..2705)
                     /locus_tag="VFC01_08845"
     CDS             complement(1764..2705)
                     /locus_tag="VFC01_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZN79776.1"
                     /translation="MTPKATNGFHRRTVEFWSGDSFCSAWLYLPDAASEAPVPVVVMG
                     HGLGATRELGLAPYAGRFAAAGMAALVFTYRHLGDSGGEPRQLLWMSKQLADWDAALE
                     FAAGLPEVDGSRIAVWGSSLGGGHAIAVAARHPELRAAVAQCPFTDGLASASALGVRE
                     SLVLFGFVVRDVLAAMRGKDPVCVPVAAAPGRPGLMTAPDALPGMQALLPHGYEWVNR
                     AAARSLLSLIRYRPGRAAKRIAAPTQICISTTDSVAPAAPTERYARQAPRGEVRLYSA
                     GHFDFYVGKAFEQLVADQTEFLVRHLRPAHVPTHLPS"
     gene            complement(2702..3667)
                     /locus_tag="VFC01_08850"
     CDS             complement(2702..3667)
                     /locus_tag="VFC01_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN79777.1"
                     /translation="MSRRIQTEELEGGVILARFVGNELGLFDREDGPELFGLVERADR
                     DPNVRAVVFTGSHPHRFISHADLQWLQEDGSVIPPVGRGVTSVVARIARQFGRNKLTR
                     WLAGKTPLTGAVQLDHMHQTFVRMTTSSTIFVAALNGSALGLGAEISWACDLRLMAEG
                     DYFIGHLEVLLGFPPGAGGTQRLPRLIGSHRALVAILEGRPFSADEALAVGAIDEIVP
                     ADQLITRAVERAKYLAARPTKAIAAIKRAVNVGGSLAMSEGLHLERTEFLALLPERQA
                     QEIMLSYVRDTAQDGELPVYQPGGYDAALLHGNAAIGTTSKVVRK"
     gene            3890..4600
                     /locus_tag="VFC01_08855"
     CDS             3890..4600
                     /locus_tag="VFC01_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HZN79778.1"
                     /translation="MAKPRKESAPRLAPEQAIERLVAAATTLLAEKGPSEIKARAVAE
                     AAQVSTIAVYYHLGGLPELFQAVINKGFRDLGQAFVTAPASDDPVTSLFSIALATRRF
                     AQNNPHLYDLMFGLSTRGSYRPLQAARSGAGARSEEFQTAYAHLVQGCVRLVESGRVR
                     RDEDPEAIAPQLWSAVHGFVTLELGDHFAQFEDPVREVLEPMMVNLTVGLGDTRELCQ
                     ASHTVALYASRAVSAEPV"
     gene            complement(4602..5030)
                     /locus_tag="VFC01_08860"
     CDS             complement(4602..5030)
                     /locus_tag="VFC01_08860"
                     /inference="COORDINATES: protein motif:HMM:NF026182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HZN79779.1"
                     /translation="MPLYQCLVPSGSIDAGERARLAEAITTVHTSVTGAPRGFVNVVF
                     TEYEPTAYFTAGVPNEASVISGTIRAGRDRATRAELLTQLSEAWIAITGQDARSLLIG
                     LNEVDPTSIMEAGLIMPAPGEESAWLERHKHELGEVLSSG"
     gene            complement(5105..5833)
                     /locus_tag="VFC01_08865"
     CDS             complement(5105..5833)
                     /locus_tag="VFC01_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009473477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN79780.1"
                     /translation="MPDRAGGAAPNGVPERLIHATIGLLAEQGPAAIKARTVASAAGL
                     STMVVYSHFGGIPELTRAVIDYGFKQLDKAFSQLPVTDDPIADLALQALTCRRVAHEN
                     PHLYDLMFGLSTRATYRPLSDPSTRLSGSSPAFRSVYAHVTAACARLVDSGRVRQQDP
                     DGVAAQLWSFVHGFITLELGETFLDSDDPVRQVLLPMGVNLTVGLGDTRERAQASHED
                     AARRYESITRGDTQQRHVAHIHRK"
     gene            5968..6945
                     /locus_tag="VFC01_08870"
     CDS             5968..6945
                     /locus_tag="VFC01_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN79781.1"
                     /translation="MTHQDNSQFEIEDRGAVLIARVDGGPHSLFGVKLANQLEELVDR
                     VDRDPSVHAVVFTGAREGRFVSHADVRWLQAEGAAAPDLGRRGASAALRLARGIDRAR
                     VPEPIVQRTPMRALAQLDQLHATFLKMNASGVIFVAALNGSALGLGAEFAWAHDLRVM
                     AEGDFFIGQPEVLLGIMPGGGGSQRLPRLIGNHKSLVAILEGKPFTPAQALANGAVDE
                     VVPLDDVVERAAELGGYLGKRTKGSVEAIKRSVYFGGSMSLSEGLHVERTEFLRTDQS
                     EAGQQLMLQYLADTQSTGELPLYRPDIYAQALATGRVPAAGSTNGAAKK"
     gene            6942..7871
                     /locus_tag="VFC01_08875"
     CDS             6942..7871
                     /locus_tag="VFC01_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN79782.1"
                     /translation="MNTAHSFTRRDISFASGQDSCAAWLYLPDGVTSPPAVILGHGLG
                     GTREMRLDAFAERFAQAGIAALAFTYRHFGDSGGQPRQLLSIRRQLADWDAAIAWVKA
                     QRDLDGSRIAVWGSSFGGGHAITVASRHPELRAAVSQCPFTDGLASALALGPTESLKL
                     LPSVARDLASMVLRRSPAMIPLAGRPGSLALMNAPDALPGYEALLPPDTTFRNEVAAR
                     VAPTAMAYRPGRAAKKVKFPILFCVSNTDSVTPPAQTLQYAQTAPFGEIKRYDAGHFD
                     FYLGEPFEALVRDQVEFLSRQLHPVSAPVGRTR"
     gene            7961..8464
                     /locus_tag="VFC01_08880"
     CDS             7961..8464
                     /locus_tag="VFC01_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN79783.1"
                     /translation="MAEVIVPWANEARFQSTAEDGRLIGPFNPALLNPAIAAPFLDLQ
                     FAEQMHTSLSPRVREVVILAVGAVWQADYELYAHLAVARKAGLSEDTARAVASGRLPD
                     GLSESELIALRLTQQLSTSHRVEEALYQDAENTFGAQGLIDIAFLIGIYHSVCAMLAM
                     FAIPAPE"
     gene            8475..9938
                     /locus_tag="VFC01_08885"
     CDS             8475..9938
                     /locus_tag="VFC01_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN79784.1"
                     /translation="MNFASLPDHRAALDPDGPAVSDPRVSLTNAGFLQRVRAAASHMT
                     TLGIGPGDVVALKLKNRVEFVVLLFAAWRLGATVTPVNPSLTDEEVVRQLDSSGARLL
                     VVEEGPATEAGVTTLAVCDLSTEPVGAEQPPHLESSALALLIYTSGTTGIPKGVMLDH
                     ANIDAMADMGRLALQVGQADRCLLILPLFHVNGIVVSVLIPLLAGGSVVIADRFDPAT
                     FFDTIEDERPTFFSAVPTIYTMLAALPAEVVPDTSSVRFAVCGAAPAPAELLTRFESR
                     YGFPLIEGYGLSEGTCASTINPVAGPRRAGTVGIAFPGQQIRIIDESGVEVATGVDGE
                     VTIAGPNVMRGYLGRPDETARVIVDGWLHTGDIGHLDADGYLTLVGRSKDMIIRGGEN
                     IYPKEIEDVLSSDASVLEVAVIGVPDEKWGEVVAAYVQPRSGLTVDPQALHALCARKL
                     AGYKRPTSITVVDAIPKNAVGKTDKAPLRAAHSKGRP"
     gene            9938..11182
                     /locus_tag="VFC01_08890"
     CDS             9938..11182
                     /locus_tag="VFC01_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HZN79785.1"
                     /translation="MSSLHDPLRLPCGQVLPNRIMKAALSEGLADKQNSPDVRLERLY
                     TSWSQGGYGLIVTGNVMVDRTQLGEPGNVVIEDESNLDALSRWAKTTKDAGVPIWVQL
                     NHPGRQSNPLALGHTPVAPSPVALNMPGAPTPRELTSAEIEDIIERFAASAKVCETAG
                     FDGVQIHGAHGYLVAQFLSPLSNQRDDEWGGDPERRMRFVIEVVRRIRARVSPGFAVG
                     IKLNSADFQRGGFTEDESRSVVAALAREGIDLIEVSGGSYEQPAMMGKAADSTRAREA
                     YFLEYARTVRNVAGDIPLAVTGGFRSRAAMQEAVNTGECDVIGLGRPTATSTDAAEVI
                     LAGRADTLKSHQVRAGMRGILGRFVDLKSLDGFLEISWHADQLHRVSAGLKPDLNRGR
                     IATLIAMLRRNGTASFGRRRGL"
     gene            11184..12020
                     /locus_tag="VFC01_08895"
     CDS             11184..12020
                     /locus_tag="VFC01_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN79786.1"
                     /translation="MTRYSLSDKVVLVTGAGRGLGAATATALARRGACVVIADIDLSA
                     AQSVAATLPTGHGLALHCDVTDLASVQDAVRQTREQFGRIDVVIANAGVLGRGGTMRA
                     LRPDQVAGVLSVNVDGVVNTVSATLDSVIENRGQIVVVSSVFAYINGAGAIPYAMSKA
                     AVEQLGRGLGVELASHGASAMTAYFSLIETDMIRHGVDQDPHLLALLSATPKFFLKRI
                     QPQTAAAAIVDGLERRKRSIAIPGRWRPFAALRGVFGPTLDAKSARDAKLQRALIQLE
                     NR"
     gene            complement(12124..12477)
                     /locus_tag="VFC01_08900"
     CDS             complement(12124..12477)
                     /locus_tag="VFC01_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79787.1"
                     /translation="MNILNTGGLTAEQAEALFNLANNGVLTRIGERGKSCEDLYQWGL
                     RPHTAETGSALAPNRLTPPTPVPGAPRFCHPALRTPPIQSGHRSTSSITSYSCCGDAA
                     DVCSIRKRTLAPSFQ"
     gene            complement(13238..14575)
                     /locus_tag="VFC01_08905"
     CDS             complement(13238..14575)
                     /locus_tag="VFC01_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN79788.1"
                     /translation="MTRTLLRNAIVLTMDDDIGDFARGDVLIEDGVISRIGVDLTADA
                     HVLDCTDKIVLPGFVNSHLHMFQTALRGYWSDALADDYFAQSRTGRDAIFHHYTPEDV
                     YWSEFVGALQNLSAGTTTVVDTSQCSYTPAHTDAALDAIRRSGIRCVFSLTPVMGHDA
                     APTYNYPNDIRRLAETTGPADTGARVSLALGYLADATDFRVARELDLPVFAHINNEWF
                     GRALEELEGEGLLGPRNTYIHCLGLQDSTWQAIARTGGKVSLSCFVEHALGMGAPAIQ
                     SAIDYNIPAGLSTDGASLGPVDMFSQMRAAFQSQRNVIHESARKDPPENAKPVTTRDI
                     LRMATLRGAQAAHLDHLIGSLTPGKIADIIVLNARTLNTVPMSHATGAVVHHMDTSNI
                     DTVIVGGEIVKSHGRLHHVDVDAALTELTRTAENLVRRSRYPDILLTSCRHFP"
     gene            complement(14572..>15329)
                     /locus_tag="VFC01_08910"
     CDS             complement(14572..>15329)
                     /locus_tag="VFC01_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZN79789.1"
                     /translation="RLAESYRRGRVFIAGDAAHVHPPAGAQGLNTGIQDAYNLGWKLG
                     QVLAGAPPELLDTYETERQPIAAGVLGLSTEKYSGLGKMDPSSIRRGEDEQQLSLSYR
                     GGPLTAATDERTATLQAGDRAPDAVLRDGDGKPVRLFDVYRGTHFTALAYGEQAAREL
                     EQLSWPTTGAPLRRVTIGTDTALTEKLLLDPAATFKKIYGLDADTLLLIRPDGYIASI
                     ATRDMLTQTSSAITRLAPPVRIVAPATPEESQP"
BASE COUNT         2695 a         4810 c         4993 g         2831 t
ORIGIN      
        1 ggctcacctc ggtcgagcca gtaggcgaag cgactgttgt cttggccgaa aaacgttggt
       61 tcgaactggc tttccgatac cgcgcgcaga cgctgccagg tggtggtgtc gccgatctcg
      121 cgtgccatcc acaagccgtt cgacagcatc tgggggtcgt cggccagctg tacgacgggc
      181 accttcgggt tgctccagcc cagctgacgc gtcgctgact cgtacaggtc gatggcgagt
      241 tgcgggtcct gcggatagag gtagtgcagg aggcagagcg cgctgtaggc gttcagcggg
      301 ccggggaacg ccatcgtcgc gttctcgatg gggtcgtaat acaacgccac ccaatcgatc
      361 cggccgcggc ggtcccggcc catgtagtgc ttcttggcga acgcgatcca atccaggaac
      421 ggctcgttga gcgacgtgtg cagcagcgag tcatagagct tcaggcccag accggccgcg
      481 ctcatgcaga acggccagat cttggtgttt tcgcaatgcg ctccttgagg tcgcgctgtg
      541 agctggtcgg agatgaactg cgcgatccgc tggtgcgtcc aggaaaattg gcggttctgg
      601 taaccggaaa cgtcgaaggt cacgtgttgt gtcggctggc ccgacacgta gtggcgcatg
      661 ctcaacagca ggttgagcca cccgcggtag aacaggttgc cgtcacagcc gaccgggtcg
      721 ggttgcagac cccacggttc gatgccgttt ccggtccagc ccggcaggga atagcggccg
      781 cgcaggtgct cgggcgcgac catcagccat tcggggggat atttgtccac gccggggtca
      841 gggccgatca gggtgttcca atcgatggcg ccccagaatg tcacgtagcg ccgcgcgatc
      901 tcgtcgagga tgcgggtgta gccctcgcgc catgccggag tcgcctccaa catcatgggc
      961 aatacgtaac ccatcatggc gaggtcaaag cgcgggaacg agcacatggg gggctcgctg
     1021 tagcgatccc accactgatg gggttcgcct tggtcggacc aatcgtcgtc ggtggtcgcc
     1081 ttgtcccata agaacctcag ccagcccagc gatcggtcgt tgagtccgcc ttgggtcgtc
     1141 gacgtaacgg attgccgctc gaatgttgtt gtcatgtcag ggctccagca tgggttcgtc
     1201 ggtgcaggca cggaccgtcg acaacgtcga gcgcgctttc caggcacccg tccacgtggg
     1261 atctagccgt acgaccgcgg gttcgatgac gatcgtctcg gcgccgcacg cggcgggatc
     1321 gtgggcgatg tacgtgccgg ggatgcgatg gcgaggtccg tctgggcggc cgagtttgac
     1381 gaactttcgg gtcaggccgt ggaaccacag cagcacgaaa gcgctgccgt gaagctcgac
     1441 ggtcccttca tggaggccgc gaacgatctt gtcgaccgtc acgggggata cgagcgcgcg
     1501 cagcgcttgc caactcgagt tgatgtgcac tgcgcaatcc ggtgccgagg catgcccgga
     1561 atatgttgtg gctcgccgta actgcgcgtc gaacacccag tgccgggcga cgcgctcacc
     1621 ggcggacaac tcgaaggtca ttgttcgcgt gatctgggaa cgcagcgacg gcatgtaccg
     1681 agatccccgc gcgatgcact gcccgagtaa ccacagcagc gttcgaagtg ccgcatcagc
     1741 catcgcttat ccctcggaga ccgtcagctc ggtagatggg tcgggacatg tgcgggtcgg
     1801 aggtgtctga ccaagaattc ggtctgatcg gccaccagtt gttcgaaagc ctttccgacg
     1861 tagaagtcga aatgtcctgc gctatagagc cgaacctctc cgcgcggcgc ctgtcgtgcg
     1921 taacgctccg tcggcgcagc aggtgcgacc gaatcggttg tgctgataca gatctgggtc
     1981 ggggctgcga tgcgcttggc ggcccggccg ggccgatacc ggatcaggct caacaagctg
     2041 cgggcagcag cacggttcac ccactcatag ccgtgtggca gcagcgcctg catccctggc
     2101 agcgcatcgg gtgcggtcat gagccccggc cgtccgggtg cggccgccac ggggacacag
     2161 accggatcct taccccgcat cgctgccagc acatcgcgga ccacgaaacc gaagagcacc
     2221 aatgattccc gtacgccgag cgcgctcgca gaagccagcc catcggtaaa ggggcactgg
     2281 gcgacggcgg cgcgcagttc tggatggcgc gcggcaacgg caatggcgtg gcctccgccc
     2341 agcgaacttc cccagaccgc gatccggctg ccgtcgactt ccggcaggcc ggctgcgaac
     2401 tcgagtgcgg cgtcccaatc ggctagctgc ttgctcatcc acaacagctg gcgtggctcg
     2461 ccgccgctat cgccgagatg gcgataggtg aacacgagtg ccgccattcc ggcggcggcg
     2521 aatcgcccgg cgtagggcgc caaacccagt tcccgagtag cgcccagacc gtgccccatc
     2581 acgacgaccg gcaccggcgc ttcggatgcg gcgtctggca ggtacagcca ggccgagcag
     2641 aaggagtcgc cggaccaaaa ttcgactgtg cggcggtgga atccgttggt ggccttgggt
     2701 gtcatttcct cacaaccttg cttgtcgtgc cgatcgcggc gtttccgtgt agaagggcgg
     2761 cgtcgtatcc gccgggctga tacaccggca gctcgccgtc ttgtgcggtg tcgcgcacgt
     2821 agctcagcat gatttcctgg gcctgccgtt ctggcagcaa ggccaggaat tcggtgcgtt
     2881 ccagatgtag gccctcgctc atcgccaacg atccgcccac gttcaccgcg cgcttgatgg
     2941 cggcgatcgc tttggttggt cgggcggcca gatacttggc ccgctcgact gcccgggtaa
     3001 tcagctgatc ggcggggacg atctcatcga tcgccccgac tgccagcgct tcgtcggcgg
     3061 agaacggcct accttccaga atggccacca gcgcacgatg tgaaccgatg agccgaggca
     3121 ggcgctgggt tccaccggct ccgggcggaa aaccgaggag tacctcgaga tgcccgatga
     3181 agtaatcgcc ttcggccatc aaccggaggt cacacgccca tgagatctct gcaccgagcc
     3241 cgagcgccga cccgttgagg gcggccacga agatggtgga actggtggtc atccgtacga
     3301 acgtctggtg catgtggtcg agctggaccg ccccggtcaa cggcgtcttg ccggccagcc
     3361 agcgggtgag tttgttgcga ccgaattgcc gggcgatccg cgcgaccacg ctggtcacac
     3421 cacggcctac cgggggaatg accgaaccgt cctcttgtaa ccactgcagg tcggcgtggc
     3481 tgatgaagcg gtgcggatgc gacccggtaa agaccacagc gcggacgttg ggatcgcggt
     3541 ccgcgcgctc gacgaggccg aacaactcgg ggccatcctc acggtcgaac agccccagtt
     3601 cattgccgac aaatcgcgcg aggatcaccc caccctcgag ttcttcagtc tggatacgcc
     3661 gagacatgac gtcgccctcc ttacccccat tgatgcggcg cagcccccgg gcgccgcttg
     3721 tataactttg ttataaactt tgttataacg atagtgtgct gggtcacacg acgcaacccc
     3781 cggatttagc ggtgggcgat gggccgtgcg cctgcgagcg agtgcactgc ggtgcttgta
     3841 acctcgttct agagggaaag ccggtctaca caggaggtat gcggccgcaa tggcgaaacc
     3901 ccgtaaggag tccgcaccac gactggcacc cgaacaggcc atcgagcggt tggttgcagc
     3961 ggccaccacg ttgctcgccg aaaagggtcc gtccgagatc aaggctcgag ctgtcgccga
     4021 ggccgcgcag gtctcgacga ttgcggtgta ttaccacctc ggcggactgc ccgaactgtt
     4081 ccaagccgtc atcaacaagg ggttccgcga cctggggcag gcctttgtca ccgcgccggc
     4141 gagcgacgat ccagtcacct cacttttctc aatcgcgttg gctacccggc gattcgcgca
     4201 gaacaatccg cacctctatg acttgatgtt cgggctctcc acacggggaa gttatcgacc
     4261 actgcaggcg gcacgttccg gcgcaggagc ccgatcggag gagtttcaga ccgcgtatgc
     4321 gcatttggtc cagggatgtg tgcggctcgt ggaaagcggt cgcgtccgcc gcgacgagga
     4381 tcccgaggcc atcgcgcccc aactgtggag cgccgtgcac ggcttcgtca ccctggaatt
     4441 gggggatcac ttcgcccaat tcgaggatcc ggtccgtgag gtactggaac cgatgatggt
     4501 taacttgaca gtcggactgg gcgacacccg ggagttgtgc caggcctcac acaccgttgc
     4561 gttgtatgcc tcgcgcgcgg tgagcgctga gccagtgtga gctatccgct ggagagcact
     4621 tccccgagtt cgtgcttgtg gcgctccagc caagccgatt cctcgccggg agcgggcatg
     4681 atcagcccgg cttccatgat cgacgtcgga tcgacctcgt tgagtccgat caacaggctg
     4741 cgagcgtctt gcccggttat cgcaatccac gcttcggaga gctgagtcag aagttcggct
     4801 cgcgttgcac ggtctcggcc cgcccggatg gttccgctga tcaccgacgc ctcgttgggg
     4861 acacctgccg tgaagtacgc ggtcggctcg tattcggtga acaccacgtt gacgaatccg
     4921 cggggcgcgc cggtgaccga cgtgtgcacg gtggtgatcg cttcggcgag gcgggcacgt
     4981 tcgccggcat cgatcgatcc ggacgggacc aagcattgat agagcggcat tgatcgtcct
     5041 tccggcactg ctagtcgtga cctcgagtat agaacttcgt tataacgcgt acaggttccg
     5101 ggcgtcactt ccgatggatg tgcgcaacat ggcgctgctg ggtgtcgcct cgcgtgatcg
     5161 attcgtaccg acgcgcagcg tcttcatgtg aagcctgggc gcgttcgcgc gtatcaccca
     5221 gccccacagt gagattcacc cccatcggaa gtagcacctg ccggaccggg tcgtcggagt
     5281 cgagaaaagt ctcgcccaac tccagggtga tgaagccgtg cacgaaactc cacaactgcg
     5341 cggccacccc gtcgggatct tgttgtctga ccctgccgct atccacgaga cgtgcgcacg
     5401 cggccgtgac gtgagcgtag acgcttcgga atgccggcga actcccactc aagcgtgtgc
     5461 tcggatcgct cagtggccgg taggtggcgc gcgtggacag tccgaacatc aggtcgtaca
     5521 ggtgggggtt ctcatgggcg acccgtcgac aagtcaacgc ctgcaaggcc aggtctgcga
     5581 ttggatcgtc ggtcaccggt agctgggaaa acgccttgtc cagctgcttg aatccgtagt
     5641 caatgacagc gcgtgtcagc tcgggaatcc caccgaaatg gctgtagacg accatcgtcg
     5701 agaggccggc cgctgacgcc acggtgcgcg ccttgatcgc ggccggtcct tgctctgcca
     5761 gcaggccgat cgtggcgtga atgagccgct cgggcacacc attgggcgct gccccgcctg
     5821 ccctgtccgg catgttccct ggctccttcc ccgggtgaca gtccgtttcc atgacccttg
     5881 tcacactcgg aataactttg ttatagtatc ggataacttc gttatagcac gtccacaacc
     5941 cgcgaaccct cacggaagaa gtcagccatg acacaccaag acaacagcca attcgaaatc
     6001 gaggaccgcg gagccgtcct tatcgcccgt gtcgacggcg gcccgcacag cttgttcggc
     6061 gtcaaactcg ccaaccagct cgaggagttg gtcgaccgag tcgaccgcga tccgagcgtc
     6121 cacgccgttg tgtttaccgg cgcgcgggaa ggcagattcg tcagtcacgc cgacgtgcgg
     6181 tggctgcaag ctgagggtgc ggcggctccg gacttgggcc gacgcggcgc ctcggccgcc
     6241 ctgcgactgg ccagaggaat cgaccgcgcc cgcgtgcccg agccgattgt gcagcgaacc
     6301 ccgatgcggg cactggccca gctcgaccaa ttgcacgcga cgtttctgaa gatgaacgcc
     6361 agcggcgtca tcttcgtcgc cgccctgaat ggttcggccc ttggtctggg cgcggaattc
     6421 gcctgggccc acgatctgcg cgtcatggcc gaaggggact tcttcatcgg tcagcccgag
     6481 gtcctgctcg ggatcatgcc cggcggtggc ggcagccagc gcttgcctcg cctgatcggc
     6541 aatcacaagt cgctggtcgc gatcctcgag ggcaagccct tcacccccgc gcaggcgctg
     6601 gccaacggcg ctgtcgacga ggttgtgccg ctcgacgacg tcgtcgagcg ggcggccgaa
     6661 ctcggcgggt accttggcaa gcggaccaag ggatcggtcg aggccatcaa gcggtcggtc
     6721 tacttcggcg ggtcgatgtc gttgtcggag ggcctacacg tcgagcgcac cgagttcctg
     6781 cgcaccgatc aatccgaggc cggtcaacaa ttgatgttgc agtaccttgc ggatactcag
     6841 tccaccggtg agctaccgct gtaccgtccg gacatctacg cgcaagctct ggcgacgggc
     6901 cgggtacccg cggccggctc gaccaacggg gcggcgaaga aatgaacacc gcccactcct
     6961 tcacccgacg tgacatctcc ttcgcctccg gccaggactc gtgtgccgct tggctttacc
     7021 tgccagacgg ggtcacgtcg ccgccggcgg tgatcctcgg ccatggcctc ggcggcaccc
     7081 gtgagatgcg actggacgcg ttcgctgagc gtttcgccca ggccggcatc gcagcgttgg
     7141 ccttcaccta ccggcacttc ggcgacagcg gcgggcagcc tcgtcagctg ctgtcgatca
     7201 ggcgacagct cgccgactgg gatgccgcga ttgcgtgggt gaaggctcaa cgagatctcg
     7261 acggatcacg tatcgcggtg tggggcagct cctttggcgg cggccatgcc atcaccgtcg
     7321 cgtcacgcca ccctgaactg cgagctgccg tgtcccagtg cccgttcacc gacggtctag
     7381 cctcggcgct cgcgctgggc ccgaccgaat ccctgaagct tctgccatca gttgcccgag
     7441 accttgcgtc catggtgctg cgccggtcgc ccgcgatgat cccgcttgcc ggcaggccgg
     7501 gttcgctggc gctgatgaac gcgcccgatg cccttcccgg atacgaggcg ctgctgccgc
     7561 ccgacacgac gttccgcaac gaggtagcgg cgcgggtggc cccgacggcc atggcctatc
     7621 gacccgggcg tgcggcgaag aaggtcaagt tcccaatcct gttctgtgtc agcaacaccg
     7681 acagcgtcac gccgcccgcc cagaccctgc agtacgcgca aactgcaccg ttcggcgaga
     7741 tcaaacgcta cgacgctggc catttcgact tctacctcgg cgagcccttc gaagcactgg
     7801 tgcgcgacca ggtggagttc ctcagccgcc agctgcaccc cgtgtccgcc ccggttggac
     7861 ggactcggtg atgtccggcg acacggatgc gttgggcggc cgcctgccac tgactgatcc
     7921 cgccgcgctg agcccggctc aacgggaaat cttcgaccga atggcggagg tgatcgtgcc
     7981 atgggccaac gaagcccgct tccaaagcac cgcagaagac ggccggctca tcgggccgtt
     8041 caaccccgct ctgctcaacc ccgccatcgc cgcaccgttt ttggacttgc agttcgccga
     8101 acagatgcat acgtcgttga gtccgcgtgt gcgcgaagtg gtcatcctcg cggtaggcgc
     8161 ggtctggcag gccgactatg agctgtatgc ccacctggcc gtagcccgga aggcgggact
     8221 ctcagaagat accgcccggg cagtggcaag tggtcggctg ccagacggcc tgagtgaaag
     8281 cgagctgatc gccctgcggc tcacccagca actgtcaacc agtcaccggg tcgaagaagc
     8341 gctgtatcaa gatgccgaaa acacgtttgg agcacaagga ttgatcgata tcgccttcct
     8401 catcggcatc taccactcgg tgtgcgcgat gctcgccatg ttcgcgatac ctgcgcccga
     8461 gtaggagagc cgcgatgaac ttcgcttcat tacctgacca tcgcgccgcg ctggatccag
     8521 acggacctgc ggtctccgat ccgcgggtgt cgctgaccaa cgccgggttc ttgcagcgcg
     8581 tccgagcggc ggcctcgcac atgaccaccc tgggtatcgg tcccggcgat gtggtggccc
     8641 tcaagctcaa aaaccgagtc gagttcgtcg tgctgctatt cgcggcatgg cggctgggcg
     8701 ccaccgtcac gccggtaaat ccgagcctga ccgacgaaga agtggttcga cagctcgata
     8761 gctccggggc acgcctgttg gtggttgagg aaggccctgc gacagaggca ggtgtcacca
     8821 cgcttgccgt ctgcgacctg tctaccgaac cggttggtgc cgagcagccg ccccatctcg
     8881 agtcttctgc gttggccttg ctcatctaca ccagtggcac gacggggatt ccgaagggag
     8941 tgatgctcga tcacgccaac atcgatgcga tggccgatat gggacggctg gcgctgcagg
     9001 tcggccaagc agatcggtgc ctgctgatcc tgccgctgtt tcacgtcaac ggcatcgtgg
     9061 tcagcgtcct gataccgctg ctcgccggtg gcagtgtcgt cattgccgac cgcttcgatc
     9121 ccgcgacctt cttcgacacg atcgaggacg agcgacccac cttcttcagc gcggtgccga
     9181 cgatctacac catgctcgcg gcgttgccgg ccgaggtcgt acccgacacg tcgtcggtgc
     9241 ggttcgcggt ctgcggtgca gcacccgcac ccgcggagct actgactcga ttcgagtcac
     9301 gatacggatt tccgttgatc gaggggtacg ggctgtcgga gggaacgtgc gcctccacca
     9361 tcaaccccgt cgcaggtccg cgacgtgccg gaaccgtagg aattgcgttc ccgggtcagc
     9421 agattcgaat catcgacgag agcggtgtgg aggtggccac gggtgtcgat ggtgaggtca
     9481 ccatcgctgg ccccaacgtc atgcgcggat atctcggacg gcccgacgaa accgcgcgcg
     9541 tgatcgtcga cggttggctg cacaccggcg acatcggtca cctcgacgcc gacggctacc
     9601 tgacgctggt cgggcgatcc aaagacatga tcattcgcgg tggggagaac atctacccca
     9661 aggaaatcga ggacgtgttg agcagcgacg ccagcgtcct ggaagtcgcc gtgatcggcg
     9721 tacccgacga aaaatggggc gaggtggtgg ccgcctacgt gcaaccacgc tccggcctga
     9781 ccgtcgatcc tcaagcactg cacgcgcttt gcgcgcgcaa gctcgccggc tacaagcgcc
     9841 ccacctccat caccgtcgta gacgccattc ccaagaacgc cgtcggcaag accgacaagg
     9901 ctccgctacg tgctgcccac tcgaaaggac gcccctgatg tcttccctgc acgacccgtt
     9961 gagattgccc tgcggccagg tgctgcccaa tcggatcatg aaggccgcgt tgagcgaagg
    10021 gctcgccgac aagcagaact cacccgacgt cagactcgag cggctgtata cgagctggag
    10081 ccaaggtggg tacggtctga tcgtcaccgg caatgtgatg gttgatcgta cgcagctagg
    10141 tgagccgggc aatgtcgtca tcgaagacga gagcaacctg gatgcgctct cgcgctgggc
    10201 caagaccacc aaagacgccg gcgttccgat ctgggtgcag ctcaatcatc ctggacgcca
    10261 gtcgaaccca ctcgcgctcg gacacacgcc ggtggcgccc agcccggtgg cgttgaacat
    10321 gcctggcgcg cccacgccac gcgagctgac atctgcagag atagaagaca tcatcgagcg
    10381 tttcgccgcc tcggcgaaag tctgcgagac ggcgggcttc gacggtgtcc agattcacgg
    10441 tgcgcacgga tacttggtcg cacagttcct ctcgccgttg tcgaaccagc gcgacgacga
    10501 atggggaggt gaccccgagc ggcggatgcg tttcgtgatc gaggtcgtgc gccgtatccg
    10561 cgcacgagtg tcgccgggat tcgcggtagg catcaagctc aactcggccg atttccagcg
    10621 cggcgggttc accgaagatg aatctcgttc cgtggtagcg gcattggccc gcgagggcat
    10681 cgacctgatc gaagtcagcg gtggcagcta cgaacagccc gcgatgatgg gcaaggccgc
    10741 cgacagcacc cgagcgcgcg aggcctactt cttggagtac gcccgcacgg tgcgcaatgt
    10801 ggccggcgac attccgctgg cggtcacggg cgggttccgt tctcgcgccg cgatgcagga
    10861 ggcagtcaac accggtgagt gcgacgtcat cggtctgggc cgccccaccg cgaccagtac
    10921 cgacgccgct gaggtgatct tggccggccg cgccgatact ctcaagtcac accaggttcg
    10981 tgccggaatg cgcggcatcc ttggcaggtt cgtcgacctc aaatcgctcg acggctttct
    11041 agaaatttcg tggcacgccg accaactgca tcgagtaagt gccggcctca agcccgacct
    11101 caatcgcggc cgcatcgcga ccctgatcgc gatgctgcgc cgcaacggca ccgcgtcctt
    11161 cgggcgcaga cggggtcttt gacatgacgc gctattcgct gtcggacaag gtcgtcctcg
    11221 ttaccggagc tggccgcggg ctgggcgcgg ccaccgccac ggcgctagcc cggcgcgggg
    11281 cgtgcgtggt gattgcagat atcgatctgt ctgccgcgca atcggttgcg gcgaccctcc
    11341 cgaccggcca cggtcttgcg ctgcactgcg atgtcaccga cctcgcctcg gtccaggatg
    11401 ccgtgcgcca gacccgtgag cagttcggac gtatcgatgt cgtgatcgct aacgccgggg
    11461 tgcttggccg cggtggcaca atgcgcgctc tgcgccccga tcaggtggct ggtgttctgt
    11521 cggtgaatgt ggacggcgtc gtcaatacgg tgtcagccac actggattcg gtgatcgaaa
    11581 accgcggaca gatcgtcgta gtgtcgtcgg tgtttgccta catcaacggt gcgggtgcaa
    11641 tcccgtatgc gatgagcaag gccgccgtcg agcaactcgg ccggggcctt ggcgtcgaat
    11701 tggccagcca cggcgcgtcg gcgatgacgg cgtacttctc gctcatcgag accgacatga
    11761 tccgtcacgg cgtagaccaa gatccccact tactcgcgct gctctcggcg acacccaagt
    11821 tcttcctcaa gcgaattcag ccgcagactg cggccgctgc gatcgtcgat gggctggagc
    11881 gccggaaacg ctccattgct atccccggac gctggcggcc attcgctgcg ctgcggggtg
    11941 tgttcggacc tacgctcgac gccaagtccg cccgcgatgc gaagcttcag cgcgccctga
    12001 tccaacttga gaaccgatag cccgaaggac tgcggcgcgc tgtagccgac ccggccgcgt
    12061 ctacgaaaca cgttgtccat cagagtgttc agcgccttga tccaacagtc ccgatgacca
    12121 acactattgg aacgaaggag ccagtgtgcg tttccggatt gaacagacat ctgctgcatc
    12181 gccgcagcag ctgtacgacg tgatgctgga tgtcgagcgg tggccggact ggatgggcgg
    12241 tgtccggagg gcggggtggc agaagcgggg cgctcccggc accggagtcg gcggagtgag
    12301 acgattcggt gctagggcgc tcccggtctc ggcggtgtgc gggcgcagcc cccactggta
    12361 caggtcttca cagcttttgc cgcgttcgcc tatgcgggtg aggacgccgt tgttggcgag
    12421 gttgaacagt gcctcggctt gctcggcggt cagcccgccc gtgttcagga tgttcatggg
    12481 gtaacggtag gcagcgggta cgacagcaca ccgctgctcc gccgcggggt cgcgttcccg
    12541 caaaagcagc ccatccgaaa cgggctgctg aatgtctctc ggcgacttgt cgtcctcgcg
    12601 acggcatgtg acgtatcgac gaccgccaag cacgaccaac cacgcatgcc ctgaactgga
    12661 cttttgcaac gaagattaac aagcctccgc gacacgcccg agttccgtca aagtagccag
    12721 gcgtgtcttg tcaattttcg ctgcatctcg acagcggcgg ctgtcgatat gcagcgaggt
    12781 ttgacaaccg gtgcggattg tttaacaacg ggtgtctgag actctgagag cgcgaaagac
    12841 gcgtatctcg aacggaagtc gccatgcaac gaatgttgac aacggcgtga gacacgccga
    12901 tggtctgtca gagtagctcc agtcggtgtt cgcggacgtg tcggtgcgag gcatcggtta
    12961 ggcgtgcgag ccgattggag atggtccctt tggaggcggc cgctgcggtc acacaggtcc
    13021 actaggatca ttgaaccatc gctgatgcgt tgcagccgaa cagcatccag tggagttaca
    13081 gctcttggat gggtcgtcca tcgtcgcgga tcgtgcagtg cgccaattac ccggcgatga
    13141 gctggtccac cagccagatc ctcggctgct tctggcatct cctgcaagct cgaagtcggg
    13201 atagcggagt tcctgaaagg acgcaatcgc tgatcggtta cgggaaatgg cgacatgagg
    13261 tcagcagaat gtcgggatat cgtgaacgac gcacgaggtt ttcggcggtt ctggtcagtt
    13321 cagtgagggc ggcgtccacg tcgacgtggt gaagccgccc atggcttttc acgatttcac
    13381 cgccgacgat gacggtgtcg atgttgctgg tgtccatgtg gtggaccact gcaccggtcg
    13441 cgtgcgacat cgggacggtg ttgagcgtgc gggcgttgag cacgatgatg tctgcgatct
    13501 tgccgggcgt gagggatccg atgagatggt cgaggtgtgc cgcttgcgcg ccgcgcagtg
    13561 tcgccatccg aaggatgtcg cgagttgtga ccggttttgc gttttcgggc gggtccttcc
    13621 gggcggattc gtggatcacg ttgcgttgcg actggaacgc ggcccgcatt tgggagaaca
    13681 tgtcgaccgg tcccaagctg gcgccgtcgg tgctcaagcc ggcggggatg ttgtagtcga
    13741 tcgccgactg tattgcgggg gctcccatgc cgagggcgtg ctcgacgaag caggacagcg
    13801 acaccttgcc gccggtgcgc gcgatggctt gccaggtcga gtcctgcaga ccgaggcaat
    13861 ggatataagt gttgcgtggg cccaacagtc cttcaccctc gagttcttcg agtgcacgac
    13921 cgaaccattc gttgttgatg tgtgcgaata cgggcagatc gagttctcgg gcgactcgaa
    13981 agtccgtggc atcggctagg tagcccagcg ccaaactcac gcgcgcgccg gtgtcagcgg
    14041 ggcccgtcgt ctcggccagt cgtcgaatat cgttggggta gttgtacgtc ggcgccgcat
    14101 cgtggcccat aaccggggtg agactgaaaa cgcaacgaat gcccgaacgc cggatggcgt
    14161 cgagcgcggc gtcggtgtgc gcaggggtgt aggagcactg cgatgtgtca acgaccgtcg
    14221 tcgttccggc ggagaggttc tgaagggcgc ccacaaactc actccaatac acatcctcgg
    14281 gcgtgtagtg gtgaaagatc gcgtcgcgtc ctgtcctcga ctgggcgaaa tagtcgtcgg
    14341 ccagcgcatc agaccagtag cctcgcagcg cagtctgaaa catgtgcagg tgagaattga
    14401 cgaaacccgg caacacgatt ttgtcagtgc aatccagcac gtgagcgtcg gcggtgagat
    14461 cgaccccgat gcggctgatc acgccatcct cgatgagcac atcgccacgc gcgaagtcac
    14521 cgatgtcgtc gtccatcgtc agcacaatcg cgttgcgcag tagggtgcga gtcatggctg
    14581 cgattcctcc ggcgtcgccg gggcgacgat ccgcaccggc ggggcgagcc tggtgatggc
    14641 tgagctggtc tgtgtgagca tgtcgcgggt ggcaatgctt gcgatgtagc cgtcgggccg
    14701 gatgagcagg agagtgtcgg cgtccaggcc gtagattttc ttgaatgttg cagccgggtc
    14761 gagcaatagc ttctcggtga gggcggtgtc agtgccgatg gtgacccgcc ggagcggggc
    14821 gccggtggtg ggccacgaca gctgctcaag ttcgcgcgcc gcctgttcgc cgtacgccag
    14881 cgcagtgaaa tgcgttcccc gatagacatc gaacagtcgc accggcttac cgtctccgtc
    14941 acgcagcacc gcatcggggg cgcggtcacc cgcttgcagc gtcgcggtgc gttcgtctgt
    15001 ggctgcggtg agcgggccgc cacggtagct cagcgacagc tgctgttcgt cctcgccccg
    15061 ccgaatgctg gaggggtcca tttttcccag cccgctgtac ttctcggtgg acaggccgag
    15121 tacccccgcc gcgatgggtt ggcgttctgt ctcgtaggtg tcgagcagtt caggtggggc
    15181 accggcgaga acctggccga gtttccagcc gaggttgtag gcgtcctgga tgccggtgtt
    15241 cagcccttgg gcgcctgcgg ggggatgcac gtgtgcggca tcgcctgcga tgaaaacccg
    15301 cccgcgccgg tagctctctg ccagccgaa
//
LOCUS       DASRJA010000198         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3252758,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000198 DASRJA010000000
VERSION     DASRJA010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3252758"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VFC01_08915"
     CDS             <1..206
                     /locus_tag="VFC01_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN79790.1"
                     /translation="EIEALKGIGMSPTEALGAASWTAREWLGRPGLEHAASADLVCYA
                     DDPRSGVEVLNHPARIILRGRIF"
     gene            complement(301..1137)
                     /locus_tag="VFC01_08920"
     CDS             complement(301..1137)
                     /locus_tag="VFC01_08920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZN79791.1"
                     /translation="MRKLLAALTIALSAAVMVAPPVSAESGVQPAGAVDLPQGPAQAW
                     LLADLDTGRILASRNPYEPHAPASTIKALLAMVVLDQLPLNAVIAAKPANTDVECSCV
                     GVKEGRAYTVRQLLDGLMLVSGNDAANTLAEGLGGYQAAVARMNAKAAALGVRNTHAS
                     SPSGLDGPGMESVTTPFDLAMIYRAALHYPAFAAIVRQPSSLFPTDDGPKKIVNQNEL
                     LARYPGTLVGKTGYTDLAQHTFVGAAQRNGHSLVVVQMYGNDDMYGQAIGLLDWGFSQ
                     YG"
     gene            complement(1200..2078)
                     /locus_tag="VFC01_08925"
     CDS             complement(1200..2078)
                     /locus_tag="VFC01_08925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZN79792.1"
                     /translation="MGRFVAVIGACLSLVLLSLTPVATAEPDIQPVGSVPIPDGPAPA
                     WIVADMDTGQVLAGRDMDVAHPPASTIKTLLALTALDELPNLDATVVGTEADTHVECN
                     CAGIKPGRTYTARQLLDAVLLVSGNDAANALADMIGGYGTAVAKMNAKAAAIGAMNTH
                     ASTPSGLDGPDGPGFTTPRDLAIIFRAAMANPTFAQITSQPVAMFPSDTGEKPLVNED
                     ELLHRYPGTIGGKTGFTDAAKKTFVAAADRGGRRLVIAMMYGLIKEGGPTYWDQAAGL
                     LDWGFAQDSTASVGSL"
     gene            2136..2555
                     /locus_tag="VFC01_08930"
     CDS             2136..2555
                     /locus_tag="VFC01_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN79793.1"
                     /translation="MLSLEEISDRFEIQQLLIDYSTAIDNRRFDDLDRVFTPDAYIDY
                     RAMGGIDGRFPEVKAWLAQVLPNFPAYAHMLGNFDVRVDGDTASSRTICFNPMVLGGE
                     QNQILYCGLWYDDEFIRTPEGWRMNRRVETKCFDKVV"
BASE COUNT          459 a          869 c          864 g          474 t
ORIGIN      
        1 acgagatcga ggccctcaag ggcatcggga tgagcccgac cgaggcgttg ggggccgcat
       61 cgtggaccgc tcgtgagtgg cttgggcgcc ctggcctgga gcacgccgct tcggccgatc
      121 tcgtgtgcta tgccgacgac ccccgttcgg gcgtcgaggt gctcaaccac ccggcgcgaa
      181 ttatcttgcg cggccgcatt ttctagcgcg agcatacgca aagtgcccca aatcctcggc
      241 gtgtcgggga cttttgcgtt ccccagctga cgcgtgggcc cagctcgcac aatgaaggct
      301 tcagccgtat tggctgaagc cccaatcgag taggccgatt gcctggccgt acatgtcatc
      361 gttgccgtac atctgcacga ccaccaggct gtggccgttg cgctgtgcgg cgccgacaaa
      421 cgtgtgctgg gccagatcgg tgtagcccgt cttgccaacg agggtgccgg gatagcgcgc
      481 cagcagctcg ttctgattga cgatcttctt cggaccgtcg tcagtgggga acagcgacga
      541 cggctggcgc acgatcgcgg cgaaggccgg atagtgcagc gcggcacggt agatcatcgc
      601 cagatcgaac ggcgtcgtca ccgactccat ccccggaccg tccagccccg acggcgacga
      661 ggcgtgggtg ttgcgtacac ccaacgcggc cgccttggcg ttcattctcg cgacggcggc
      721 ctgataaccg ccgagtccct cggccagggt attggcggcg tcgttgcccg acaccagcat
      781 cagcccgtcg agcagctgac gcaccgtgta ggcccgaccc tccttgactc ccacacacga
      841 acactccacg tccgtgttgg cgggtttcgc ggcgatcacc gcattgagcg gaagttgatc
      901 cagcaccacc atcgccaaca gcgccttaat tgtgctcgcc ggtgcatgcg gctcatatgg
      961 gttgcgcgaa gccaggattc gtccggtatc caggtcggcc agcagccatg cctgggccgg
     1021 gccctgcggc agatccaccg cgcccgcggg ttgaacgcca gactcggccg acaccggggg
     1081 agcgaccatc acggccgcgg acaacgcgat ggtcaacgcg gccaacaact ttcgcacgtg
     1141 cgccgagagt actcagcgca ttcgccgacg aggtctcgga tgcgttgcga ttgcgcaact
     1201 tagagtgagc cgacgctggc cgtcgagtcc tgcgcgaagc cccagtcaag cagcccggct
     1261 gcctgatccc agtacgtcgg gccgccctct ttgatcagcc cgtacatcat cgcgatcacc
     1321 agtcgacgtc cgcctcggtc agcggcagcg acgaacgtct tcttggcggc atcggtgaaa
     1381 ccggtcttgc cgccgatggt gccggggtag cggtgcagca actcgtcctc attgaccagg
     1441 ggcttctcgc ccgtatcgga cgggaacatg gccacgggct gcgaagtgat ctgggcgaat
     1501 gtcggattgg ccatcgcggc gcggaagatg atcgccaggt cgcggggcgt ggtgaacccc
     1561 gggccgtccg gcccgtccag ccccgatggc gtcgacgcgt gtgtgttcat cgccccgatc
     1621 gcggccgctt tggcgttcat cttcgccacc gcggtgccgt agccgccgat catgtcggcc
     1681 agcgcgttgg ccgcgtcgtt acccgacacc agcagcacgg cgtccagcag ctggcgcgcc
     1741 gtgtacgtgc ggcccggttt gatgcccgcg cagttgcact cgacatgtgt gtcagcctcg
     1801 gtaccgacga cagtcgcgtc caggttgggt agctcgtcga gcgcggtgag cgccagcagc
     1861 gtcttgatcg tgctggccgg cggatgcgcg acgtccatgt cgcggccggc gagcacctgg
     1921 ccggtgtcca tgtcggcgac gatccacgca ggagcggggc catcggggat cggcacgctg
     1981 ccgaccggtt ggatgtccgg ctcggccgtc gcgaccggcg tcagggacag aaggacaaga
     2041 ctgagacaag cgccaattac agcgacgaac ctccccatgg aacaggcagc ctaaccctcg
     2101 cggctgaaag cgctccaatc gtgttcaatc aacgcatgtt gagcctggag gagatttcgg
     2161 accggttcga gattcagcag ctgctgatcg actactcgac ggcgatcgat aaccgacgat
     2221 tcgacgacct cgaccgcgtg ttcaccccag acgcctacat cgactaccgg gccatgggcg
     2281 gcatcgacgg gcgttttcca gaggtgaagg catggttggc gcaggtgctg ccgaacttcc
     2341 cggcctacgc ccacatgctg ggcaacttcg acgtccgtgt tgacggcgat accgcgtcat
     2401 cccggaccat ctgcttcaac ccgatggtgc tcggcggtga gcagaatcag atcttgtact
     2461 gcgggttgtg gtacgacgac gaattcattc gcacgcccga gggctggcgc atgaatcgtc
     2521 gcgtcgaaac caagtgcttc gacaaggtgg tgtgattcag gccgcccgct gaccaacgaa
     2581 caagtagcgc gggatcggct tgtcgtcgat ccggctctgc ccgacctgca ggcccgaatt
     2641 ctcaagggct gcagcgattt cggcga
//
LOCUS       DASRJA010000199         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1561385,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000199 DASRJA010000000
VERSION     DASRJA010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561385"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFC01_08935"
     CDS             <1..338
                     /locus_tag="VFC01_08935"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZN79794.1"
                     /translation="PPPPPPPPPPPPPPPNTAPVVNAGPDETALTGLLYTLSWSFSDG
                     TPNGPWTYTINWGDGSTSTGTVSGQGSFSTGHTYVTILPRTYTITVTVRDAAGLSGSD
                     TKVVSVLLP"
     gene            621..1181
                     /locus_tag="VFC01_08940"
     CDS             621..1181
                     /locus_tag="VFC01_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79795.1"
                     /translation="MLRIVFTPDMGSNMEPSVHSLGLPSVRTIYTAWAIRLSPNWDDS
                     PWAAGAMTALFADGPDQIYTALFHPCAWPEVCGPEAYGPPYKIGASTEWALEEQQVRY
                     PNVTTTWINPGEWHLVEFYYQWSTSALAADGIIRWWVDGTLNGDYSNVHYPAGSFVEL
                     QFAPTLQDAPPAEQYMYIDHTRVSVP"
     gene            1397..>2031
                     /locus_tag="VFC01_08945"
     CDS             1397..>2031
                     /locus_tag="VFC01_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79796.1"
                     /translation="MLGQSGTSLGIAFPANPHVGDAIVATFFWYGPAGIVNSVTDRLA
                     DGTSVGNVYRLATSFTAGPVSMATYVATNAQNFPDPNPDGTKRVVVEAHLSSSVPQGG
                     VIVSAYTGVQADPLQAVGAISSNSGSGTSATDAAPGPITVAPGSLVYGVTVADAFVGL
                     GRPPAPFAPVFQASDDSLPMKADAEAAVLASGGAVEPRWNWGFSSGSPKTW"
BASE COUNT          330 a          660 c          676 g          365 t
ORIGIN      
        1 caccgccgcc gccgccgccg ccgccgccac caccaccgcc accgccgaat acggcgccgg
       61 ttgtgaatgc gggaccggat gagaccgcgc tgacaggtct cctgtatacg ctgagctgga
      121 gcttcagcga tgggacgccg aatggaccgt ggacgtacac gatcaactgg ggcgatggct
      181 caacatcgac cgggaccgtc tcggggcagg gctcgttcag tacgggccac acctatgtga
      241 cgatcctgcc gcggacgtac acgattacgg tgaccgtacg tgatgcggcc gggctgtccg
      301 ggtccgacac gaaggtcgtc tcggtgctgt tgccgtaggg ctcaatgcgc tctcgcattc
      361 tcctgttgct gctggtcgtc ggcgcggcgt gcgaacgggc ggaaccgccc ctcgcacccg
      421 cgccggtccc tccgtcagtg acgccaggtg cgctgccgaa tgaaccggtt gggttccaga
      481 cattaagcga ccagtcgtgg agttgtccgt cgccgtttgc ggagacgatt gcctgcaatg
      541 ggtggggtta cgcaccgcga tcgagcacta agcctgatga cgtcatcgcc gacgccacgg
      601 cgcccgtctc tgcaggcgac gtgttgcgga ttgtgttcac cccggacatg ggcagcaaca
      661 tggagcccag cgtccactcg ctcggtcttc cgtctgtcag aacgatctac acggcgtggg
      721 cgatcagact ctccccgaat tgggacgaca gcccctgggc ggcgggcgcc atgaccgcgc
      781 tgttcgcgga cgggccggac cagatctata ccgcgctctt tcatccctgt gcctggcccg
      841 aggtgtgtgg tcccgaggcg tatggcccgc cgtacaagat cggcgcgagc acggagtggg
      901 cgctcgaaga gcagcaggtc cgctacccca atgtgacgac gacgtggatc aaccccggtg
      961 aatggcacct cgtcgagttc tactaccagt ggtcgacgtc ggcgctcgct gctgatggga
     1021 tcatccggtg gtgggtcgac gggacgctca acggtgacta cagcaacgtg cactacccgg
     1081 cgggcagctt cgtcgagctg cagttcgctc cgacgctgca ggacgcaccg cccgccgagc
     1141 aatacatgta catcgatcac acgcgagtca gcgtgccctg agtcccggtg gctgtgacgt
     1201 cgcaccggtg ctcacgatgt cggtagccaa ctagccacag aggcacctca tgcggcggcg
     1261 aacggggttg tccagaatag tggtggcggg cctgctcgcc gcagcctgca gtgatgtact
     1321 caccccgggg cgtgagccta tcgggcccgg ggggccgctg tttgatgttg cggcgacccc
     1381 cacccagcaa aacggcatgc tgggacagag cggcacgtcg ctggggatcg cgttccctgc
     1441 caacccgcac gtcggcgacg cgatcgtcgc gacgttcttc tggtacggcc ctgcagggat
     1501 cgtcaactcc gtgacggatc gactggccga cggcacgtcg gtcggtaacg tgtatcgcct
     1561 agccacatcg ttcactgccg gtcccgtctc gatggcgacg tacgtcgcca ccaacgccca
     1621 gaacttcccg gatccaaatc ccgatgggac caagcgcgtg gtggtcgagg cgcatctgtc
     1681 gagttcggtc ccgcaaggtg gcgtcatcgt ctcagcctac acgggcgtgc aggccgatcc
     1741 gttgcaagcg gtgggcgcta tcagctcgaa ttcaggctcg ggcacgtcag ctaccgatgc
     1801 cgcgccagga ccgattacgg tcgcgcctgg ttcgctggtt tatggcgtga ctgtggcgga
     1861 cgcgttcgtg ggccttggcc ggcctccggc tccgttcgcg ccggtcttcc aggcctccga
     1921 cgactcgctg ccgatgaagg ccgatgcgga agctgcggta ctggccagcg gcggcgcggt
     1981 cgagccgcgt tggaattggg ggttcagctc gggctcaccg aaaacgtggt t
//
LOCUS       DASRJA010000200         7183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1671516,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000200 DASRJA010000000
VERSION     DASRJA010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671516"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="VFC01_08950"
     CDS             <1..1509
                     /locus_tag="VFC01_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN79797.1"
                     /translation="SWLLGKSTESPARRRVRIQVILTVLILATNMLGIGIAILLVTVV
                     FPVPSIFSDAPLWIALAVSPAYTALALAVGTFWITRRTVNTMRWAIEEQAPTRQDQLN
                     TFLAPWRVAKAVLILWGVGAALLTTLYGLQDVAFIPRFLFSVGFSGVVVATACYLLTE
                     FALRPVAAQALEAGPPPRRLSGGIMGRTMTVWLLGSGVPVVGILLTGVFALSLQNLTE
                     AQFAFAVMILAVIALFFGFVLMWILSWLTATPVRVVRAALKHVEQGDLDCNVVVFDGT
                     ELGELQRGFNSMVDGLKERERVRDLFGRHVGREVAAAAEQQRPKLGGEERHVAVIFVD
                     IIGSTQLVTGRPATEVVDLLNRFFAVIVEEVDRHHGFVNKFEGDAALAVFGAPNRLDN
                     PEDEALAAGRAIARRIRDEVPECEAGIGVASGEAVAGNVGAKERFEYTVIGEPVNEAA
                     RLCELAKSTSSHLLASSETVQNAGENECGHWTFGDTVTLRGHDEPTRLALPV"
     gene            1665..1967
                     /locus_tag="VFC01_08955"
     CDS             1665..1967
                     /locus_tag="VFC01_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79798.1"
                     /translation="MSFKTAVSASALAAGIGAAGLFGLGLGTASADPWGCDRPGTPSC
                     GEHRDDRGPGDWHGRGIDQGRFDHQPFDWMGQQVFPVPAGNGAGWGFWFLGQWIPL"
     gene            complement(2018..2791)
                     /gene="modA"
                     /locus_tag="VFC01_08960"
     CDS             complement(2018..2791)
                     /gene="modA"
                     /locus_tag="VFC01_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZN79799.1"
                     /translation="MVLAMRGGFKQTMVAGILLAISASGCTSQPHPTTSITVFASSAM
                     IESLTAIGKQFEAENPGTSVEFIFSGSSDLSAQLADGNDADVFVSGDHDNMAAVANAG
                     LLAAPPVPIAANSLVIAMAPGDHDKPGSVADLARPGLRLAICGGPGACGSATRQLQDH
                     TGVQLQPHNIDTTGIDVLKDLTSGKADAGLVFKTDALNAGDNVAWFALPEADVDAITS
                     WIAPMKDSDHGDLATKFIHDVTGPTGRKILADDGFAEPS"
     gene            2916..3422
                     /locus_tag="VFC01_08965"
     CDS             2916..3422
                     /locus_tag="VFC01_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79800.1"
                     /translation="MSDIGGLGMRLSARISVAPVNDSFKRLPARDVTFRLGRVLLMVE
                     GLAIGVLGGVALAWSMGYTHFSEDGIPMLGLKVTPPHAGLLLIVGTLALLACLGRRTA
                     LIFSALTAFGWAALTIVCAFKVAHHTPGVLGFDPRDGLLYCALCAYNLVTSLLLAAAL
                     VKHHRRAS"
     gene            complement(3415..3642)
                     /locus_tag="VFC01_08970"
     CDS             complement(3415..3642)
                     /locus_tag="VFC01_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79801.1"
                     /translation="MRATCVSPVGKRRSCRTTTTTTTTTTAPPSPSSAPPAPTEKSIN
                     PTGGNLFTPPVVAPPAPTVPPGQHPGINGVS"
     gene            3803..3964
                     /locus_tag="VFC01_08975"
     CDS             3803..3964
                     /locus_tag="VFC01_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79802.1"
                     /translation="MRKEEAVHGTVKDDDLDLLVSLDRCDDFIELRNTFWPKDIQRWQ
                     IERNSSVRR"
     gene            4155..4673
                     /locus_tag="VFC01_08980"
     CDS             4155..4673
                     /locus_tag="VFC01_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN79803.1"
                     /translation="MTPTRYRTVTVDGLNLFYREAGPSDAPAVLLLHGFPSSSRMWQP
                     LIDRLADRYRMVAPDYPGFGHSDAPPADSFSYTFDRIADYIERFADEIGLQSYSLVVQ
                     DYGGPVGMRLALRRPERLSALVVHNAVCHEDGLGPLWETRRAFWADRASHEQALRESF
                     FSPQRNSSAARR"
     gene            4804..5052
                     /locus_tag="VFC01_08985"
     CDS             4804..5052
                     /locus_tag="VFC01_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN79804.1"
                     /translation="MWQQWLRDAQPPLLVVWGRYDPSFQVEEAEAYRRDVPDAEVHVI
                     DAGHFALDEAPDLVADLTRNFLDASERQHQPPAHPAGF"
     gene            complement(5008..5160)
                     /locus_tag="VFC01_08990"
     CDS             complement(5008..5160)
                     /locus_tag="VFC01_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN79805.1"
                     /translation="MASGVTQLRDLVEQQRVSMHVADVDPAEKAADAHRCLEAGGVRG
                     GLVLTF"
     gene            complement(5496..5882)
                     /locus_tag="VFC01_08995"
     CDS             complement(5496..5882)
                     /locus_tag="VFC01_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79806.1"
                     /translation="MNVPVQYYQSLIAIETAVTGVLLFQIRFFEDADAPGRSASDPRV
                     RVLMLIVLTATLFGSLEAIREGGGLEVAVLVTVGLAVSILPILLRVLPPLRRDAETHQ
                     RDPHFWITVLGLIVYAAIVALIVSID"
     gene            complement(6012..6509)
                     /locus_tag="VFC01_09000"
     CDS             complement(6012..6509)
                     /locus_tag="VFC01_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HZN79807.1"
                     /translation="MTGSLWWDLVIGVGSALMLAWLALVIALAILRPRGGLLREALRV
                     LPDVLRLVRRLAADRTLPRGIRIRLGLLLAYLALPIDLIPDFIPILGYADDAIIVTAV
                     LRSVVRRAGVDAVRAHWPGTDDGFIVLARLTGITGPTTQIDDTDRATLGDRGPVDGVG
                     DRGAR"
     gene            complement(6506..7102)
                     /locus_tag="VFC01_09005"
     CDS             complement(6506..7102)
                     /locus_tag="VFC01_09005"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HZN79808.1"
                     /translation="MIDAHRRGWLVISVSTAGAAGTLRVQVWRKLRSLGALYLQQSVC
                     LLPARAEVVREVRRLADRIHHQGGTVRILRMSFADPAEEHAVIEELNTAREAEYAEVL
                     ERLPALRHELAEETARGRLTYAEVEESEADLDRFRTWLARIAARDYFSAPGGQAAREA
                     VEDAAAELAAFEEAALHADAPEPTAAVKTQQLRIVEEQ"
BASE COUNT         1273 a         2265 c         2282 g         1363 t
ORIGIN      
        1 tcctggctgc taggcaaatc cactgaaagc ccggcacgca ggcgggtccg cattcaggtc
       61 atcctgacgg tgttgatcct ggccacaaac atgctcggca tcggcatagc catactgctg
      121 gtcaccgtcg tgttcccggt cccgagcatc ttcagtgacg cacctttgtg gatcgctctg
      181 gcagtgtccc ccgcctatac ggctctcgcg ttggcggtgg ggacgttctg gatcactcga
      241 cgcacggtca acacgatgcg atgggcgatc gaggagcagg cgccgacgcg gcaggaccag
      301 ctcaacacct ttcttgcccc gtggcgggtg gcgaaggccg tcctgatcct gtggggagtt
      361 ggggccgcgc tgttgacgac gctgtacggg ttgcaggatg tcgcattcat tccgcgattc
      421 ctgttctctg tcggcttcag cggagtcgtc gtcgcgacgg cgtgctactt gctaacggaa
      481 ttcgcactgc gcccagtggc tgcacaggcg ttggaagcgg gcccgccgcc gcgccggctg
      541 agcgggggaa tcatgggccg gaccatgacg gtctggctct tgggatcggg tgtgccggtc
      601 gtcggcatct tgctgaccgg ggttttcgcg ctctccctgc agaacctgac cgaagctcaa
      661 ttcgctttcg cggtaatgat tttggcggta atcgcactgt tcttcggttt cgttctgatg
      721 tggatcctgt catggctcac cgcaacgcca gtgcgggtgg tgcgagccgc gctcaagcac
      781 gtcgaacagg gcgatctcga ctgcaatgtg gtggtattcg atggcaccga actcggcgag
      841 ttgcagcgcg ggttcaactc gatggtggac ggcctgaaag aacgagaacg agtgcgagac
      901 ctgttcggcc gacacgtcgg acgtgaggtg gccgcggccg cggagcaaca gcgccccaag
      961 ctgggtggcg aagaacgcca cgtcgcagtg atattcgtcg acatcatcgg ttcgacccag
     1021 ctggtcaccg gccggccggc gacagaagtc gttgacctgc tgaaccggtt cttcgcagtg
     1081 atcgtcgagg aggtcgaccg ccatcacggc ttcgtcaaca agttcgaggg ggatgcggcg
     1141 ctggcggtgt tcggtgcccc gaaccgcctc gacaatcccg aggacgaggc gctggcggcc
     1201 ggccgtgcca ttgcgcggcg gatccgcgac gaggtgccgg agtgcgaagc gggaatcggc
     1261 gtggcgtcag gtgaggcggt cgccggaaat gtcggtgcca aggagcgttt cgagtacacc
     1321 gtcatcggag agccggtcaa cgaggcggcc cggctgtgtg aattggccaa gtccacgtcc
     1381 tcacacttgc tggcgtcatc ggagacggtg cagaacgccg gcgaaaacga atgtggccat
     1441 tggactttcg gcgacacggt gacgctgcgt gggcacgacg agcctacccg gcttgcctta
     1501 ccggtatagc ggcctgatca atcccgttga tagcaaacgg ttgtgaatct gtaatcgctt
     1561 gcacagccgg cacacatcgt caccacagtg ccaaacaatg tccgctcctt aacgtcgttt
     1621 tgcgtggcaa gcactgccgc atggacgaga aacggagtga ggaaatgtct ttcaagactg
     1681 cagtatcggc atcggccctg gccgccggaa tcggggccgc tggactgttc ggcctcggcc
     1741 tcggaacggc cagtgcagat ccgtggggtt gcgaccgtcc gggcactccg tcgtgcggtg
     1801 aacaccgcga cgaccgtggc cccggcgact ggcacggtcg cggcatcgac cagggccgat
     1861 tcgatcacca gcccttcgac tggatgggcc agcaggtgtt ccccgttccc gcgggcaacg
     1921 gcgctggctg gggcttctgg ttcctcggtc agtggattcc gctgtaggtc aacatgattc
     1981 gaggcgggtc gcgccctggt gacccgcctc gaagaggcta gcttggttcg gcaaatccgt
     2041 cgtcggcgag gatctttcgg ccagttgggc ctgtgacatc gtgaatgaac ttcgtcgcaa
     2101 ggtcgccgtg atctgaatcc ttcatcggcg caatccatga cgtgatcgca tcgacgtcgg
     2161 cttcgggaag tgcgaaccat gcgacgttat ccccggcgtt cagcgcatcg gtcttgaaca
     2221 ccaggccagc gtcggctttc ccactcgtga ggtccttcag gacatcaatg cccgtcgtat
     2281 cgatgttatg tggttgaagc tgcacacctg tgtggtcctg gagctgtcgg gtcgccgagc
     2341 cacaggcacc gggtcctcca cagattgcca accgcaggcc tggtcgggcc aggtcggcga
     2401 ccgagccagg tttgtcgtgg tcgccaggag ccatggcaat gaccagactg tttgccgcga
     2461 taggcaccgg aggtgcagca agcaggccgg cgtttgcgac ggctgccata ttgtcgtggt
     2521 cgccggatac gaacacgtcc gcgtcattgc cgtccgccag ctgcgccgag aggtccgaag
     2581 agcctgagaa gatgaattcc actgacgtcc cagggttttc ggcctcgaac tgcttgccga
     2641 tcgcggtcag gctctcgatc atcgccgacg acgcgaacac ggtgatggac gtcgttgggt
     2701 gcggctgcga cgtacaaccg ctcgccgaga tcgccaacaa aatcccggcc accatcgttt
     2761 gcttgaatcc gccgcgcatc gctagcacca tacctgctcg caaccagtga agtagcttgc
     2821 cgtccacatg tctatgttgt ggagatgcgc cgcatctgct gcgaattagc ttgcgttata
     2881 cccgtaatcc gtcgcttcac ggcacgctgg aatacgtgag cgatatcgga ggcttgggca
     2941 tgcggctgtc cgcgcgcata tccgtcgccc cggtaaacga ttccttcaag cgacttcccg
     3001 cgcgcgatgt caccttccgt ctggggcgtg tcttgttgat ggtcgagggc ctggcaatcg
     3061 gggtgctcgg cggtgtcgcg ctggcctggt cgatggggta cacccacttc agcgaggacg
     3121 gcataccgat gctcggtctc aaggtgacgc cgccgcacgc ggggcttctg ctgattgtgg
     3181 ggacgctggc cctactggcc tgccttgggc gacggaccgc acttatcttc agcgcactca
     3241 cagcattcgg ttgggcagca ctcactatcg tctgcgcgtt caaggtggca catcacacgc
     3301 ccggtgtgct cgggttcgat ccgcgcgacg gacttctcta ctgcgcattg tgcgcctaca
     3361 acctggtgac gtcgttactg ctggcggcgg cgctggtcaa gcaccaccgt cgagctagct
     3421 gacgccgttg atccccggat gttggccggg aggaactgtc ggtgcaggag gggcgacgac
     3481 tgggggtgtg aagagatttc ctccggtcgg gttgatgctc ttctcggtcg gcgccggcgg
     3541 tgcactgctg ggcgacggcg gcgccgtggt cgtcgtcgtc gttgtcgtcg tggtgcgaca
     3601 ggatcgcctt ttccctactg gcgaaacgca tgtcgctcgc aagggcgagc tcgctgccaa
     3661 cacccgtcgc ccgcccgcgg attgaagcga tggaaaccac gggtgcacga gttaaccgtg
     3721 caaacatgtc gggaagcgct tgtaggccgg tgggacccgg aggaaactgc gacgactcct
     3781 cgagcctcac cacaaagtcg tagtgcgtaa ggaagaagcc gtccacggca ctgtcaaaga
     3841 cgacgacctt gacctgctcg tcagtctcga tcgctgtgat gatttcattg agttgcggaa
     3901 tactttctgg cccaaagata ttcagcggtg gcagatcgaa cgtaactctt cagtacgccg
     3961 gtgatcgtct cgtcagtcga atctgcttgg cggtcgttgc ctcggtcatg gatcgctcct
     4021 cgtgtacctg gcactctgct cgacggctga taggtaattg aatgccggct ggattgcatt
     4081 gtcctgcgag atcgctggga atgtgctgcg aagcatgagg acaggagcag ctgctcaggt
     4141 aggttcgagc tgccatgacc ccgacccggt atcggaccgt caccgtcgat ggcctgaatt
     4201 tgttttaccg cgaggccggc ccatccgatg cgccggcggt cctgctgtta cacggtttcc
     4261 cgtcgtcatc aaggatgtgg cagccgctta tcgaccgact cgccgaccgc tatcgaatgg
     4321 tggctcccga ctatccggga ttcggacaca gcgatgcgcc gcccgccgat agcttcagct
     4381 acacgttcga ccgtatcgcc gattacatcg aacggttcgc cgacgaaata gggcttcaga
     4441 gctactcgct cgtcgtccag gactacggtg gacccgtcgg catgcgactc gcgctgcgcc
     4501 gtcccgaacg gctcagcgca ctcgtcgtgc acaacgcggt ctgccatgaa gacggcctcg
     4561 gaccgctttg ggaaacccgg cgagcattct gggccgaccg cgccagccac gaacaggcac
     4621 tgcgggaaag cttcttctcc ccccaacgca actcgtcagc ggcacgtcgg taacagtcca
     4681 gaacccgacc gctacgaccc cgacctgtgg accgacgaac tcgcgttcct ccaccaaccc
     4741 ggccaacgcg acatccagac cgacctgttc tacgactacc gcaccaacgt cgcgagctac
     4801 ccgatctggc aacagtggct ccgcgacgcg cagcctccgc tgttggtggt gtggggtcgc
     4861 tacgatccct cattccaagt cgaggaggcc gaagcgtacc gacgcgacgt gcccgacgca
     4921 gaggttcatg tcatcgacgc cggccatttc gccctcgatg aagcccctga cctcgtcgcc
     4981 gatctgaccc ggaatttcct tgacgcttca gaacgtcagc accagccccc cgcgcacccc
     5041 gccggcttct aggcacctat gcgcgtcggc tgccttctcc gcgggatcga cgtcagccac
     5101 gtgcatcgaa acccgctgtt gctcaactaa atcgcgaagc tgagtgacac ccgatgccac
     5161 tgcacgaatt gaatctttcg tcgcgacgtg cttctcatgt cgtcgtcgac gccctgaatc
     5221 gagatcgcga ccatcggaat accatcgaac taatctggta tccagcggta cgctccacgc
     5281 ccggaagcag ccgatctcgt gccgctgtcg agatgcagcg aagattgacc agactcaccg
     5341 gcccactttg acaagtcttc ggcgtgtcgg gggagcctgt caaagtatgc gatcgcagtt
     5401 tcagttccga ccgatttgat tgtggagtca ggtccagatc ttgggcttcg gcggtgagta
     5461 agccgcgctg gcacgtctgg atggccgacc tgcgatcagt cgatcgagac gatcaatgcg
     5521 acgatcgccg cgtacacgat gaggccgagc acggtgatcc agaaatgggg gtcccgctgg
     5581 tgcgtttctg cgtcacgtcg cagcggaggc agcacccgta gcaggatcgg caatatggag
     5641 accgcgagac ccacggtgac cagcaccgcg acctccagac cgccaccttc gcgaatggct
     5701 tcgagcgaac cgaacagtgt cgcggtgagc acgatcagca ttagcacccg gacccgcgga
     5761 tcactggccg atctccccgg tgcgtcggcg tcttcgaaga accggatttg gaacagaagg
     5821 acaccggtga cggccgtctc gatcgcgatc agcgactggt agtactggac agggacgttc
     5881 acgatcacct ccggggggcg agcgatgttt gatgagtgcc cgcgaggcta acgcctcgcc
     5941 cgggcgcttc aatcgtcagc cgcgccacca cttcggcgat cattgtcgac tcactgatct
     6001 gggccaccac gtcagcgggc gccacggtcg ccgaccccgt caaccggacc gcggtcgccg
     6061 agagtggccc gatccgtatc gtctatctgg gttgttgggc cggtgatacc agtgagtcgg
     6121 gccagcacaa tgaatccgtc gtcggttccg ggccaatgcg cgcgtacggc gtcgacgccg
     6181 gcgcgtcgga cgacgctacg caggacggcg gtgacgatga tggcgtcgtc ggcgtagccg
     6241 aggatcggga tgaagtcagg gatcaaatcg atcgggagcg ccaggtaggc cagcagcagc
     6301 cccaggcgga tccggatccc gcggggcagg gtcctgtccg cagcgaggcg gcgcaccagc
     6361 cgcagcacgt cgggcagcac ccgcagggct tcgcgcagca gcccaccccg cggccgcaga
     6421 attgctagcg caatcaccag cgctaaccag gccagcatca gcgcactgcc gacgccgatc
     6481 acgagatccc accacaacga gcccgtcatt gctcctccac gatgcgcagc tgctgcgtct
     6541 tcaccgcagc ggtcggttcg ggtgcgtcgg catgcagcgc ggcctcttcg aacgcggcca
     6601 gctcggctgc cgcatcctct acggcctccc gggcggcctg accgccgggg gcactgaagt
     6661 agtcgcgggc ggcgatcctg gccagccagg tgcggaagcg atccaggtcg gcctcggact
     6721 cctccacctc ggcgtaggtg agtcggccac gggcggtttc ctcggccagt tcgtggcgca
     6781 gcgcgggcag ccgctccagg acttcggcgt attcggcctc gcgggccgtg ttgagctcct
     6841 cgatgacggc gtgttcctcg gcgggatcgg caaaactcat ccgcaggatg cggacggtgc
     6901 cgccctgatg gtggatccgg tcggctagcc gccgcacttc gcgcaccacc tcggctcggg
     6961 caggcagcag gcacaccgat tgctgcagat acaaggcgcc gagcgaacgc agcttgcgcc
     7021 acacctgcac ccgcagcgtg cccgccgcgc ccgcggttga cacgctgatc accagccagc
     7081 cccgcctgtg tgcatcgatc acatcgccat gttacacccg taacgtatcg ctgatacatt
     7141 ggagatcacg accgagggag gcacaccagg tgggcatcgc gat
//
LOCUS       DASRJA010000201        25828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4099669,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000201 DASRJA010000000
VERSION     DASRJA010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 25828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25828
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4099669"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VFC01_09010"
     CDS             <1..552
                     /locus_tag="VFC01_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HZN79809.1"
                     /translation="IAGNTAGLIDPAGVEVVHIGSAAQLKDAVSKHAPDAHVLVMAAA
                     VADFRPAQAQHSKIKKSSDPNAAAPTIELTRTDDVLAGAVRARTDGQLPNMRAIVGFA
                     AETGDANGDVLFHARAKLRRKGCDLLVVNAVGENRAFEVDNNDGWLLASDGTESALEH
                     GSKTLMASRIVDAIVAFLHSSGG"
     gene            669..1877
                     /gene="metK"
                     /locus_tag="VFC01_09015"
     CDS             669..1877
                     /gene="metK"
                     /locus_tag="VFC01_09015"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZN79810.1"
                     /translation="MSDARLFTSESVTEGHPDKICDAISDSVLDALLAGDPKSRVAVE
                     TLVTTGQVHVVGEVTTLAKEAFADITNTVRARILEIGYDSSEKGFDGETCGVNIGIGA
                     QSPDIAQGVDTAHETRVEGAGDPLDLQGAGDQGLMFGYAIKDTPELMPLPIALAHRLS
                     RRLTEVRKNGVLGYLRPDGKTQVTVQYDGTTPVRLDTVVLSTQHAAGIDLDATLTPDI
                     REKVVNTVLADLNHDTMDTSDFRLLVNPTGKFVLGGPMGDAGLTGRKIIVDTYGGWAR
                     HGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLAERIEVQVAYAIGKAAPVGLFVET
                     FGTETVDPARIEKAITSVFDLRPGAIVRDLDLLRPIYAQTAAYGHFGRTDIELPWEQL
                     NKVDELKASV"
     gene            2305..6183
                     /locus_tag="VFC01_09020"
     CDS             2305..6183
                     /locus_tag="VFC01_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79811.1"
                     /translation="MNAAISAAAIPTRDATVGDGVHVMWTPPSSAGYSIDGWDVRRRR
                     ATERPRVQCRQLTPSELAVLHRDLRLRLPFGELRIREVPCPEPRRTARAAIGTVPQRR
                     CAAYELRLTTRHRVVQVRAGVPSALAIALRDGKAVDSRQLDDPSGIQTARFDNRDVDQ
                     VEIHCDHRATSLEVCLDVAADPGADDASWAGATTVAKNLQLPLRSVNTAVSSAAQEDA
                     LAKSRLLPGEAFEAEAFRDVADLMNAAADTGLPVWQSTVTRDEDDEPFIDVRSWPYAM
                     ALLVHPAWRRMLAFAVLDQAANLTPGQVYDYRVTGRFRRRDVEEVVHGFQAVPRGTML
                     PTTFLLGDIGLSTPEPTTVELFPEPPADSLTAMGRKGIRLTGDPCLTILFCAPVSRVV
                     LDIDPSSTFEYTAQTTGFLPGLITHSFTGSVPSKPRVTLEFADPVDTVELAGQGRLFA
                     VRDVTSAPGTSPEDIVERHAVVYGVVFADTPPLPAPAVLDTDNLQQPVLPGPASPPAA
                     LGFQLAWDPPGVGVPSMPWPPNATTPPPSEAFGYQLERREVDTGGAFGPLDPTAGPVA
                     VFGLRGTGGDPPALRPGIDLETAFPESGPATSPTGTRMTYEDPLARARAPSPPPGSRH
                     QYRIYTVDALGRRSIDPTEGSIVRLEKHQPPPQPVGPPTPSPNDVGTGVRARVLQALD
                     ADLSTADRALLGSSDNAVVIEWGWTDYERAADPHAREFRVYWQPLAPDLVYGRVTGTA
                     AYTAGQYEMAAEFDRPLPADALRGRYVSLPDFPFMVAGHPDGQSFTLRIAPNGNEPQR
                     VPAAADIMFRPRLDGSELRPPAWAERSAVIPIGAADTYQFVFRDRLTLDADQPRARVW
                     VGVSAADAESYVDDVLPAAVAHGGRNGNESAIVAAEAAARYLGRPEFTVPPPLPVVPE
                     EVTDEPAGETVTIQLDLPSLLPSVPTGSTYTLDRLNLDEVLGCIGVNPDDTIRAVLPD
                     GSSTSYALANPTDQQHMIDQIRTGTPALVENRFLMDFVMRFATDLETMWAPASLTSFP
                     FGPVTDTLPAKAARYLHRIRLVDAAGHISAGGAIAPQVVRTASLRSPAPPQLEAEYLD
                     DQLVARMRIRDTVDVSWVLLFVSDQDDARGVSQLLRLPNARDRLPDDGLRLRLPQGNL
                     LEPSVVLNARGGEEDPPDRVLTTPLPADADQQVALWAVTVTRDGIPSRCTGPLSVTAP
                     PAVLLAPAVSVTRGGGVDAVQWSTVAAPALLAIEASLDNGGTWRQVSPWLAAGVASYR
                     VPAPDGEVRYRSTVRADRGRRATGVEAVVV"
     gene            6183..9083
                     /locus_tag="VFC01_09025"
     CDS             6183..9083
                     /locus_tag="VFC01_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79812.1"
                     /translation="MVSFRAPVSGGGWSFTAASPITVPVGLRYAGLRLPVLRALRTTP
                     ADLRVSATGILTKAVTADSHPSTVIEIQVNPFMIRGVAQLIPFGIPTIYLVLEPGAPA
                     PVFEDGDSAAAGDVLASVTGVGLMMVGQDRIARDAALWARLIFDAITSAGDDPVDWSD
                     FAAAVADQTDDPPVLLLDHTGGLVTGGTVRLSAGSASATVEMTDADGGDLQRAVARIR
                     AANPSAMPFSSVFSRGSPARVGSVSDSDNDFELTVLEDAEGAATAIEVTPAGRHVMYT
                     DLRTWFAPQYANATLPAHFAPLQDYSRTNRLHPFVNGPEYYADLLEKVQEAAEMARTL
                     PAFCGLHLVGGWQTFPDADLARRSDSQTATEKPVTLEEAVTLLGNRGGSSRILSPKFF
                     QFEQGSQGEANVILFITVIAYGALSAGVLNLMRSDIIGAGILVVVFLGNGLLISWLID
                     QNGDPIELNKDAVEVLGAVQNCESVYGPHPAVYEDNPLSPPLTGSYLNQIVDAQRHFG
                     FYHQKFGVVRAGPALYGYCGGIDINPNRLDDHRHVAYGPYHDIHARVEGPAARDVELS
                     FEQRWGRDGPANSQPAFFAPGASELGTPGSDAVQVARTYFRPADSSRELAWAPNGDRT
                     IADTLERAIDAAREYIYIEEQYFTPAPAFRQLLVDKVASRSVKALIVTLPVIGDQPFG
                     EMVRSGLITALRAADGGAGIVKIGYPRRHYTVPNNTLRTSSGRLVLMADLNVGAQTIA
                     LGPPSRIPVPPFWLAVDGEYMYVVDESIAPNPDPDNMLVFEVVRDAETRLIRGGVTPA
                     GARARAHNAFAAATVVSHSSIYVHAKSILVDDVFVGIGSANINRRGHYHDGEMTLYGV
                     PQRLKADPANPVAALRRRLWAEMLDIPREIADPLLADPLAAAALFDRSPLAGNRYADI
                     EAYPTHLMQDVTGGDGLFLTLLTTIAGTAVLSTSVQDIYDGIVDPTSALEFD"
     gene            9083..15490
                     /locus_tag="VFC01_09030"
     CDS             9083..15490
                     /locus_tag="VFC01_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79813.1"
                     /translation="MAPQRTTLFTLFGDPAERWPSDHVREFSGTGDPGDPAAWLGRLN
                     NAVQVANLRTGASSDAVAAQAELSVDPSAGFAADPDGWPFVIASMPDVEFRIQPMGTN
                     QGHVQMFASLGGTGFALVLEALPVEIRLPAGLVEPHPDQPGAPDGSLEQVSGEFQPGR
                     LDDLKIVLRRGGPTSVFVHVRVTMTTSYEFDIQPAVPISFGRCGVSGIPCRAAHDFRL
                     IPSPEIAPNHIEWLRHPVDPKPSPITGPYDGQFAVRTLDVDEKSMLVRPMVEWLNSHA
                     DEKTPTAEWVLEDLVVPFFSPYVIPIPVHATTGIRRKVIDVTDRGDVYEFEQDPVHLV
                     LRHEHKLAINVASFFYRSAPLAELDDRFLPTKPEFPTTGFWDDPGLTFDAALVFGDEQ
                     TPKLNDGEPAQHAIRFGLEERWTPFIGYRREWGNDGHPTEGTTRWERFVNVLLHWEIA
                     EVVIVDIMGFKLGLSLAQYFGDDDTSFMDSALLTADLYVSMPPTGSDTSKFRLRGLNG
                     EKVAFPIEGIGWRFGSVHLEGLALPDGVIFFVYKYGLILSELGVRAEDGATYLTVSGG
                     LAVDPPKGFELAATVKGLRFRVAGSADLPKVKVDGFFLRAASGSESFLLEGGGYYREL
                     EDVEEFAFALAARLKYAEKKYYFGVDGVFGSRKVADESFRYFMVEAYYKGAVGPIAAW
                     DFTGARALFARNMVPKLRDVDREARELRYFKWYKDTNPLTVTADRRLSAWQASQDSWA
                     LGVGASASLAGLGKVIELTAFVLIVRGPAEQGVLVVAEVFALASTRPFGYAVVEVDSH
                     NDRVSVLIGVDVRVGTFVKDAPAWMNDVGAVTGTLFISNDPCTFAIGRLADSATWLGL
                     RFEVDLWLEASLVIAFCVEHVEGGPNGFGLTARIEGGIGKKGVVRLSYNAGFTLVMTS
                     FSTGSTDYALVLEIGAGIRFELFGFLRIGVSTRMAFRIVGGHPARAELTAEIRLETPW
                     FLPDVTWRLDAVYGDLRPADLSSAVQPIRSASAKELTGTQLGVHLERFDPAWNGEGVA
                     PVHSVNELSAAPRPEVQRLTNFDNDAAARPVATDATIAVTWAVAVNDRIGLGTGVAGA
                     LGTQEAGDLILTYDLVALAVRRRPRFGLQRDWSPLESKVELGVDFSNPAGPQLSGTFA
                     PQVLNKSWDLDVIINGEPAPKRLLLNSVAPYDFTFFSPQADDQLTQHHPSWPCCDHID
                     DKDLSDLFHRVHWHDAGLGEPLDAPATSRFSDSNSTLRFLRPAWTRPAVFGGLVNPTT
                     VAAARLGQPGVVARADFDEDAALCSVRIASPRATRVTVVLFNRTGKEVGRQELAVTAG
                     TFQTAVVAASGPVTRLEIRANPALATTAPWAAASAPADAVIEVDEVAYVGLRDYLDIL
                     IAETACGGGPMGGGSGKLAFLPNYEYEVSLTTRVIVAHPSAPEVTADVEEFVYFKTKG
                     MPGLNAVNRIGEEIDPYVRAAYTGGHAGVVYREEPVTLAFSEGFHVAVPISVRPPGTV
                     AEHTELMRMQLLVSPDQANRADTAYTVTSPDWIVTHPGAGTPPRSGDHVWTPVVSGSR
                     TLGSSMRSADPITVRFAAMAQRPAVTCGVPDPSQVTGTVLIAEPPDPDTGLWEPGMTY
                     TASVRRESAPFVDRRPFEPGDETALTAGTLGSGSAVWTVMDGHLDRAGDGWGFATFGD
                     ATWEHATVLVGIEPGAAAAGFGVGIGGSAGLFALVEESANTRRLVIGRRQATALNEVA
                     GADLPDGPDAAILLQVTAFDDRLRATVGDTVVEADRGEDRAGRMCLCSDGPASFASLQ
                     VNGVDMYQFRFTASRFTSFEEHIGSWSGRLDDLGPDLLGPGTTTATVAQLWATSSGDV
                     TKVMDPGAAPADRERVFAGWTAGLGLATRDDVTALGLSRYVEEAQTRALLIESPEPMD
                     FTADIAVALIGRQVRHPRPPRPRPPIFDERTLAHRLTTIARQPELPLPVRGGRPSIAD
                     AIIDVEVDRASLRLQLHSSLANAGEVAVVVTTADGALDLYRGIVRPPWLRGRPAVLVA
                     ALVGPLGHIPPGSDLAAELQAARPGALFLLTSDLIELIGRWDMLPVEVDVPVDVRVLQ
                     NGDARRALVIPITGGAASVLPVGPYRLTLSLARKRWETTDPTDDINSYQRSVTVRFDV
                     TAD"
     gene            complement(15487..16971)
                     /locus_tag="VFC01_09035"
     CDS             complement(15487..16971)
                     /locus_tag="VFC01_09035"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN79814.1"
                     /translation="MKTVPDFDTVIVGAGFSGIGTAIKLDKSGLGNYLVIEAGDGPGG
                     TWYWNTYPGIAVDIPSFSYQFSFEKSPNWTRTYAPGKELKAYAEHCVDKYGLRRKIRF
                     KTKVLGADFDDKQSLWHIRTDPGGVVTARFLVNASGVLITPKLPDIDGVDSFAGLTMH
                     TARWDHNQDLTGKRVAIIGTGASAVQVIPEIAPIVEQLTVFQRTPIWCFPKPDVPLSP
                     MARRMMRLPGGSTVQRILSQAYVELTFALPAQYFTVNPMAKKMSKVGEAYLRKQVNDP
                     QVRDKLTPRYAVGCKRPGFHNTYLSTFNRDNVELVTEPIDKITGSGVATVDGETRDVD
                     VLILATGFKVMDTDDMPTYPLTGPGGRSWSQHWDENRLQAYEGVSVPGFPNFFTVFGP
                     YGYVGSSYFALIETQTHHIVRCLKRARRRNATRVEVTKEANDRYFAEMMRKRHRQIFW
                     QDSCQLANSYYFDKNGDVPLRPATTAETYWRSRRFPLDDYVFSA"
     gene            complement(16968..18362)
                     /locus_tag="VFC01_09040"
     CDS             complement(16968..18362)
                     /locus_tag="VFC01_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN79815.1"
                     /translation="MAAPAARLSTLTMSREAITIGFDAGGGFLGRTRGHDIARFRCAG
                     RRFVSITHPDYVDHVLHRARLKYVKSNDYEPIRATAGINLLTDEGDSWAAHRTTLNPT
                     FARRHLNELVDLMIDPIDRVTAEVVPDVEFDMHATMVEATLRVVANALFSQDFGPLVH
                     SMKDLTKRGLRRTERLGRLGLWGLLPRPIYQALSWSTFSGVPLPPPLREGQKIALALD
                     AAVNGVLEERIARPTDSADLLNVLFKADNGTWPRQRVRDEALTFMLAGHETTANAMSW
                     FFYLMAQHPDARDKMLAEIDGVLGGRRPTAADLTLLPWTTACLQESQRYYSAVWIIAR
                     EAVEDDLIDGHHIRPHTTVLIPIHHIHHDERWWPDPELFDPTRFLGDAGKNRPRSAYL
                     PFGGGRRICIGQSFALMEMVLMAAIMSQRFTFDLVPDFQVELEATLTLRPKHGLHVIA
                     HQREGPADERSTDR"
     gene            18439..19470
                     /locus_tag="VFC01_09045"
     CDS             18439..19470
                     /locus_tag="VFC01_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HZN79816.1"
                     /translation="MASTTWSPDLRHPMYATRLLCEVANERGVPTADVLTGTGICADD
                     LDEPEAVITAWDEIVAVRRLLARLPDDVGVGVDVGSRFKLTHAGLLGFAAMSCATLRE
                     MFDIDMRYFSLTMLHIDLKLFDGAQDCVLELDVDHLPADVRRFFIERDLAAIVSTIGA
                     FAYEVVERYADKVTAEVSLDKDVLSPLLTLVPVENISFERAHTRLHFPRAMFDEPLPQ
                     ADAHTLELCMAQCDVLMQRNERRRGITAVVRSKLFRKSGGFPTLPEVAAELDVHPRTL
                     RRQLAEEGTSFRALLNEARSTLAVDLLCNVGLNVEEVSKRLGYTDTSTFCHAFKRWHG
                     VPPSAYPRA"
     gene            complement(19467..19829)
                     /locus_tag="VFC01_09050"
     CDS             complement(19467..19829)
                     /locus_tag="VFC01_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014981351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79817.1"
                     /translation="MTERQVLDARTAQQWEAWLERNHEAADEIWLRLMNKNAPEPGMS
                     YGEAVEIALCFGWIDSLARGHDDISRIQRFSPRKSRSAWSKSNVERVQRLIDAGRMRA
                     PGQAQIDAAKADGRWPTS"
     gene            complement(19826..20797)
                     /locus_tag="VFC01_09055"
     CDS             complement(19826..20797)
                     /locus_tag="VFC01_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN79818.1"
                     /translation="MAESPTARPGIDPTMQALLDAFPLKFTADEGVEVAREQMRQLKA
                     PPEALPDMRIEERTIDYGDITGIPVRIYWPPIAQHDNLPVVVFYHGGGWAIGDLDTHD
                     PVARAHAVGAEAIVVSVDYRLAPEHPYPQGIDDSWAALQWVGEHASELGGDPARIAVA
                     GDSAGGNISAVMAQLARDNGGPALVFQLLWYPSTTGDMTLPSMIENADGPILDRDVVA
                     AFQQWYLPPEIDWTDAANLPPTLAPANTADLSGLPPAYIGTAEYDPLRDDGARYAELL
                     SAAGVPVELHNAPTLVHGYVNFALVAPAAAEATNRGLAALKAALHRG"
     gene            complement(20826..21467)
                     /locus_tag="VFC01_09060"
     CDS             complement(20826..21467)
                     /locus_tag="VFC01_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZN79819.1"
                     /translation="MTAQTIAGIAIPDTQLVRDTTEYIRDVEDDLLFDHSRRVFLFGA
                     LQGQRRGLDPDLELLYVGAMFHDIGLTADFRDSMLRFEVDGANAAEKFLRDRGLDDAA
                     VRRVWLSIALHTTPGVPEFLDPEIALVNAGVKTDVVGVGRDDLPDEVRDAITTAHPRP
                     DFKRRILQAFADGNLHRPHSTYGTINADVLEHFDPTFVRDDFVDIILNSGWPE"
     gene            21552..22508
                     /locus_tag="VFC01_09065"
     CDS             21552..22508
                     /locus_tag="VFC01_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HZN79820.1"
                     /translation="MAADRVIVFVVFDGMTLLDVAGPAEVFAEANRFGASYRLLMASV
                     DGKDVVTSVGARLTASTSIGSVKAVDTAVISGGDHLVRRPIAPDLVAAVKDLRPRTRR
                     MASICTGSFILAKTGILDGRRATTHWRQAGLLSRAYPQISVEPDAIFVRDGDIYTSAG
                     VSAGIDLALALVEDDYGADLVRDVARSLVVYLKRAGGQSQFSALVETPPPQRLHLRAL
                     TEAIAADPSANHTVKTLAAQASLSTRQLTRLFQSELGTTPARYVDMIRIDAARSALDA
                     GRSVVDSARVAGFGSAETLRRVFVNQLGISPKAYRDRFKSTG"
     gene            complement(22492..22713)
                     /locus_tag="VFC01_09070"
     CDS             complement(22492..22713)
                     /locus_tag="VFC01_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79821.1"
                     /translation="MSDSVDIVHAFREECWNAHDPAAVDRFVVDLHLEIPESFQNVDF
                     WAVREDGKLLSNHVERAALEQLQRLTRST"
     gene            complement(22710..23660)
                     /locus_tag="VFC01_09075"
     CDS             complement(22710..23660)
                     /locus_tag="VFC01_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN79822.1"
                     /translation="MSVAEEKPAVDAILLKVLEAVPFQLTTDGGVEAARQRFRALPRR
                     QFHPDVRTENRSIDGPAGPIPIRVYWPPTDGEATLPVVVFLHGGGWSVGDLDTHDGDA
                     RHHAVGAGAVVVSVDYRLAPEHPYPAAVEDAWATTQWVAAHADELGADPDRLAVAGDS
                     AGGNLAAVMAQLARDAGGPPIRFQLLWYPATTWDTSLPSFTENAEAPLLDNASVKGFS
                     RWYVGDLDLADMPATLAPARAGDLAGLAPAYIAVAGHDPLRDDGARYAELLAAAGVPV
                     ELHNAETLIHGYLGYAGVVPAATDAMDRGLSALRAALHAP"
     gene            23659..24381
                     /locus_tag="VFC01_09080"
     CDS             23659..24381
                     /locus_tag="VFC01_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HZN79823.1"
                     /translation="MGTPYVQARTRLLWVAAAIVGACYGVFLIVTALRLPAGAELTGQ
                     FTLQPSVKALTAVLLAVAALTHPIPRERRWLIAALLFSAAGDFLLAMPWWQPSFVLGL
                     GAFLVAHLCFLAALIPLVSRSGPRLVAAAVMVLACVVLLVWFWPRLLAEEMAVPVTLY
                     IAVLGAMVCAALLARLPTPWTALGAVCFAVSDGMIGIGKFVLGSEALAVPIWWAYAAS
                     ILLITAGFFFGRASVSSARPSA"
     gene            24378..>25828
                     /locus_tag="VFC01_09085"
     CDS             24378..>25828
                     /locus_tag="VFC01_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZN79824.1"
                     /translation="MTTNRQQAEHEPIARVVPMLTVPHLDREFDYLVSAEQSDDAQPG
                     VRVRVRFHGRLVDAFILERRSDTDHVGKLGWLERVVSAEPVLTPDVRRLVDAVAARYA
                     GTRADVLRLAVPPRHANVEKQTAPELAPLTGKPVDTAAWSAYGRGEQFLAALEDGRAA
                     RAVWQALPGEHWTDRLAEAAAVTVNSGRGVLAIVPDQRDVDALYASAIRYVEADRVAA
                     LSAGLGPAQRYRRWLSALRGGARMVIGTRSAVFAPVADLGLVTVWDDGDDTLAEPRAP
                     YPHAREVAMLRAHQLRCAALIGGYARTAEAQALVRSRWAHDLVAARPVVRDRSPRVVA
                     LDDSGHEQERDPAARTARLPSLALRAARTALQAGLPVLVQVPRRGYVPALACARCRTI
                     ARCRRCTGPLSLPDRDTAGAVCRWCGREEPALRCTRCGSDAVRAVIVGARRTAEELGK
                     AFPDTPVITSGGEAMVAAVPGEPSVVVATPGAEP"
BASE COUNT         4128 a         8603 c         8607 g         4490 t
ORIGIN      
        1 atcgccggca ataccgcggg cctgatcgac cccgccggtg tcgaggtcgt ccacatcggc
       61 tccgccgcgc agctcaaaga tgccgtgtcc aaacacgcgc ccgatgcgca tgtccttgtg
      121 atggccgcgg cggtggccga cttccgtccc gcgcaggcgc agcacagcaa gatcaagaaa
      181 tccagcgacc ccaatgccgc cgcaccgacc atcgagctga cccgcaccga cgacgtgctc
      241 gccggtgctg tgcgggcccg tacggacggc caattgccca acatgcgggc catcgtcggg
      301 ttcgctgcgg aaaccggcga tgcgaacggc gacgtgctat tccacgcccg tgcgaaattg
      361 cggcgcaagg gctgcgattt gctcgtggtc aatgccgtcg gtgagaatcg ggcgttcgag
      421 gtcgacaaca acgacggctg gctgcttgca tcggacggaa ccgaatctgc actggagcac
      481 ggttccaaaa ctctgatggc cagccgtatt gtggacgcga tcgtggcctt cctgcacagc
      541 agcggcgggt aacgccaacg cagacatcac gctgcatcta agggttgcgc gagcgggtgg
      601 cgcacgcttg ggtgcaccga ggcagatata taattcgatt gactaagttt tcggaaaggg
      661 atcgaaccgt gagcgatgct cggctgttta ccagtgagtc ggtgactgag ggccaccccg
      721 acaagatctg tgacgccatc agcgactccg tgctcgacgc gctgctcgcg ggcgacccca
      781 agtcacgcgt tgccgtggag acgctggtca cgaccggcca ggtgcacgtc gtcggcgagg
      841 tcaccacctt ggccaaggaa gccttcgccg atatcaccaa cacagtgcgc gcgcgcatcc
      901 tcgagatcgg ctacgactct tcggagaagg gcttcgacgg cgagacctgc ggcgtgaaca
      961 tcggcatcgg cgcgcaatcg cccgacatcg cgcagggcgt cgacaccgcc catgagacgc
     1021 gggtcgaggg cgcgggtgac ccgcttgacc tgcagggcgc cggcgaccag ggcctgatgt
     1081 tcggctatgc gatcaaggac accccggagc tgatgccgct gccgatcgcg ctggcccacc
     1141 gcctgtcgcg ccggctgacc gaggtgcgca agaacggcgt gctgggctac ctgcggcccg
     1201 acggtaagac gcaggtcact gtccagtacg acggcaccac gccggtgcgc ctcgacaccg
     1261 tcgtcctctc gacgcagcat gcggcgggca tcgacctcga cgccaccctg accccagaca
     1321 ttcgcgagaa ggtcgtcaac accgtgctgg cggacctcaa ccacgacacg atggacacat
     1381 ccgacttccg gctgctggtc aatccgaccg ggaagttcgt gctcggcggc ccgatgggtg
     1441 atgccggcct gaccggccgc aaaatcatcg tcgacaccta tggcggatgg gcccgccacg
     1501 gcggcggtgc gttctccggc aaggatccgt ccaaagtgga ccggtcggcg gcgtacgcga
     1561 tgcgctgggt ggcaaagaac gtggtcgctg ctggcctggc cgagcgcatc gaggtgcagg
     1621 tcgcctacgc catcggcaag gcggccccgg tcgggctgtt cgtggagacg ttcggaaccg
     1681 agaccgtgga cccggcccgg atcgagaagg ccatcacgtc ggtcttcgat ctgcgaccag
     1741 gcgccatcgt ccgcgacctg gacctgctgc gcccgatcta cgcgcagacc gcggcgtacg
     1801 gccacttcgg ccgcaccgac atcgagctgc cgtgggagca gctcaacaag gtcgacgagc
     1861 tgaaagcatc cgtctgacgt tccgacgcgc cgaaatcagg ttccggtacg tttcgcgacc
     1921 ctttcctgta ctggagcctg atttcgcggc gcgcacgtgc cacggctcag cgctgaacgt
     1981 gtagtcgtcg agcgtcgccg caaaccgtca cccgtgctct ggacgcagtg cccgccgcgg
     2041 acaccgatct cgacgcggca taagtcgtcg tagcgatcga tcaagacggc tgatccgaaa
     2101 tgtattatcc ggcaatggat ccgagcccct tgaaggtccc gcatatcaca tcgatgtgct
     2161 gagcgaacgc gggaactccg acaagcgact tgctgcgtaa ctcctcgccg ccgccctccc
     2221 gcgccgtagg gtttgtatcg gagggggcat ggtgcttcgc aaccggtgcg gctggcgtcg
     2281 gggccgcgtt caatcggccc gacgatgaac gccgcgatat cggcggccgc gatacccacc
     2341 cgcgatgcaa ccgtcggtga cggcgtccac gtcatgtgga ctccgccgtc cagcgcggga
     2401 tattcgatcg atggctggga cgtgcgacgc cgccgtgcca ccgagcgccc gagagtgcaa
     2461 tgccgtcaac tcacgccgtc ggaattggcg gtcctacatc gagaccttag gttgcgactg
     2521 ccgttcggcg aactgcggat ccgcgaggtg ccgtgcccag aaccccgccg cactgctcgc
     2581 gccgcgatcg gcacggtgcc gcaacggcgg tgcgcggcat acgagctgcg gctgaccacg
     2641 cggcaccggg tcgttcaggt gcgcgccggt gtgccgtctg cgctcgcgat cgcgctgcgc
     2701 gacggtaagg ccgtcgactc ccgccagctc gacgatccat cgggaatcca gacggcgcgg
     2761 ttcgacaatc gtgacgtcga tcaggtcgag attcactgcg accaccgggc gacgtcgctt
     2821 gaagtgtgtc ttgacgtggc cgcggacccc ggtgccgacg acgcctcatg ggccggcgcc
     2881 accacggtgg ccaagaactt gcagctgccg ctgcgatcgg tcaacaccgc ggtgagttcc
     2941 gccgcgcagg aagacgcatt ggcgaaatcc aggctgctgc cgggagaagc gttcgaggca
     3001 gaggcctttc gtgatgtcgc cgacctgatg aacgcggcgg ccgataccgg tttgccggtg
     3061 tggcagagca ccgtcacccg tgacgaggac gacgaaccct tcatcgacgt ccgatcatgg
     3121 ccctacgcca tggctttact cgtgcatccg gcgtggcggc gaatgctcgc gttcgcggtg
     3181 ctcgaccagg cggccaacct cacaccggga caggtgtacg actaccgggt caccggccgc
     3241 ttccgcagac gcgatgtgga agaggtcgtc catggctttc aagccgtgcc ccgcggcacg
     3301 atgctgccga cgacgtttct gctcggtgac atcggtctga gcaccccgga gcccactacg
     3361 gtcgagctct tcccggagcc gcccgccgac agcctgactg cgatgggccg caaagggatt
     3421 cgcttgaccg gcgatccgtg tctgacgatc ttgttctgcg cgccggtcag tcgcgtcgtg
     3481 ctcgacatcg atccctcgtc aaccttcgag tacaccgcgc agaccaccgg cttcctgccg
     3541 ggactgatca cgcacagctt caccggctcg gtgccgtcga agccgcgggt cactctcgag
     3601 ttcgccgacc ccgtcgacac cgtcgagctc gcgggacagg gacggctctt cgcggtgcgc
     3661 gacgtcacct cggcgcccgg cacctcgccg gaggacatcg tcgagcggca cgccgtcgtg
     3721 tacggcgtgg tgttcgccga cacgcctccg ctgcccgcgc ccgccgtact cgacaccgac
     3781 aatctgcagc agccggtgct gccagggccc gcctcgccgc cggcggcgct tggattccaa
     3841 ttggcatggg acccacccgg agtcggcgtc ccgtcgatgc cgtggccccc taatgcgacc
     3901 actccgccgc cgtccgaagc cttcggctac caactcgaac ggcgtgaggt cgacaccggc
     3961 ggagcatttg ggccgctgga tccgaccgcc ggtcccgtgg cggtcttcgg tctgcggggc
     4021 accggtggtg acccaccggc gcttcggcca ggaatcgact tggagacggc gtttcccgag
     4081 tccggacccg ccacatcacc caccgggaca cggatgacct acgaggaccc gcttgccagg
     4141 gcgcgtgcac cgtctccgcc gccgggcagc aggcaccagt accggattta caccgttgac
     4201 gcgctgggcc gtcgatccat cgaccccacc gagggatcga tcgtgcgcct ggagaaacac
     4261 cagccaccac cgcagcccgt cggtccgccc accccgtcgc cgaacgacgt cggcaccgga
     4321 gtgcgggccc gtgtgctgca ggcgctcgac gccgatctct ccaccgccga ccgcgccttg
     4381 ctgggatcga gcgacaacgc ggtcgtcatc gagtggggat ggacggacta cgaacgcgcg
     4441 gccgatccac atgcgcggga gttccgcgtg tattggcagc cactcgcgcc ggacctggtg
     4501 tacggcaggg tgactggtac cgccgcatac accgccggcc agtacgagat ggccgccgag
     4561 ttcgatcggc cactaccagc cgacgcgtta cgcggccggt atgtgagcct gcctgacttc
     4621 ccgttcatgg tcgccggcca ccctgacggc caatcgttca ccctgcgaat cgcaccgaac
     4681 gggaacgagc cgcagcgggt tcccgccgct gccgacatca tgttccggcc ccggctcgac
     4741 ggcagcgagt tgcggccacc tgcgtgggcc gagcggtccg cggtgattcc gatcggtgcc
     4801 gccgacacct accagttcgt gttccgtgat cgtctgacgc tcgatgccga ccaaccgcga
     4861 gcccgcgtct gggtgggagt gagtgccgcg gatgcggagt cctatgtgga cgacgtactg
     4921 cccgcagccg tggcccacgg cggccgcaat ggaaacgaaa gtgccatcgt cgcagccgaa
     4981 gcggccgccc ggtatctcgg gcgtcccgaa ttcaccgtcc cgccaccgct tcccgtagtc
     5041 cccgaggaag tcaccgatga acccgcgggt gaaacagtga cgatccagct cgacctgccc
     5101 tcgctgctgc cgtcggttcc cacaggcagc acgtacaccc ttgatcggct gaacctcgac
     5161 gaggtgctcg gctgcatcgg agtcaatccc gacgacacga tacgggccgt cctgcccgac
     5221 gggtcatcga cgagctacgc cttggccaac cccacagacc aacaacacat gattgatcaa
     5281 atccgcacgg gtacaccggc actggtggag aaccggttcc tgatggactt cgtaatgcgg
     5341 ttcgccacgg atctcgagac gatgtgggcg cccgcaagcc tgacctcgtt tcccttcggt
     5401 ccggtcacag acacgctgcc ggccaaggcg gcgcgctacc tgcaccggat ccggctggtc
     5461 gacgccgcgg gccacatctc ggccggaggg gcgatcgctc cgcaggtcgt cagaacggcg
     5521 tcgctgcgct cgcccgcacc gccgcagctc gaagcggagt acctcgatga ccagctcgtg
     5581 gcgaggatgc ggatccgtga caccgtcgat gtctcatggg tgctgctgtt cgtcagcgac
     5641 caggacgatg cccgcggagt gtcccagctt ctgcggctgc ccaacgcccg cgaccgcctt
     5701 cctgacgatg ggcttcgcct ccgattgccg cagggcaatc tgctcgaacc ctcggttgtg
     5761 ctgaatgctc gaggcggcga agaggatcca ccggatcggg tgctgaccac gccgctgcca
     5821 gcggatgccg atcagcaggt agcgctgtgg gccgtgaccg tgacccgcga tggcattccg
     5881 tcacggtgca ccggtcctct gagcgtgacc gcaccgcccg cggtcctcct cgcgcctgcg
     5941 gtgagcgtga cccgcggcgg cggagtcgat gctgtgcagt ggtccaccgt tgcggcgccc
     6001 gcgttgctcg cgatcgaggc cagcctcgac aacggcggca catggcgcca ggtcagccca
     6061 tggcttgcgg ccggtgttgc gtcctaccgg gtgcctgcgc ccgacggcga agtccgctac
     6121 cgctcgacgg tccgagccga ccgtggccgc cgtgcgaccg gagtcgaggc ggtggtggtg
     6181 tgatggtcag cttccgcgct cccgtatccg gtggtggatg gtcgttcacc gcggcctctc
     6241 cgatcaccgt gcccgtcggc ctgaggtacg ccgggctgcg cctgcccgtc ctgcgtgcgc
     6301 tacggacgac gcccgccgat ctgcgggtgt cggcaaccgg aatcctgacc aaggccgtca
     6361 cggccgattc gcacccgtcg acggtcatcg agatacaggt caacccgttc atgattcgcg
     6421 gtgttgcaca gttgattccg ttcggcatac cgacgatcta tttggtgctc gagcccggtg
     6481 cgcccgcgcc ggtgttcgag gatggcgact ccgctgcggc cggagacgtg ctggcatcgg
     6541 tcaccggggt gggcctgatg atggtcgggc aggaccgcat cgcgcgagac gctgcgctgt
     6601 gggcgcggct gatctttgat gccatcacat cggcgggcga cgatcccgtc gactggtcgg
     6661 acttcgccgc cgcggtggcc gaccagacgg atgacccgcc ggtgttgctg ctggatcaca
     6721 ccggcggatt ggtgacggga gggacggttc gactcagcgc cggatcggcc tcggcgactg
     6781 tggagatgac cgacgccgac ggcggcgacc tgcaacgcgc ggtggcgcgc atccgtgcgg
     6841 ccaacccatc ggcgatgccg ttctcttcgg tgttctcgcg cggcagtcca gcacgggtcg
     6901 gctcggtgtc ggattccgac aacgacttcg agctcacggt gctggaggat gccgaaggtg
     6961 ccgcgacggc cattgaggtg acgcccgcgg gcagacacgt tatgtacacc gacctcagaa
     7021 cgtggttcgc gccgcaatac gccaacgcga cattgcccgc gcactttgcg ccgctgcagg
     7081 actactcacg gaccaaccgg ctgcacccgt tcgtcaacgg gccggagtat tacgcggatc
     7141 tgctggaaaa agtgcaggaa gccgcggaga tggcccgtac cctgcctgcc ttttgcgggt
     7201 tgcatctggt cggcgggtgg cagacctttc cggatgcaga tctggcgcgc cggtccgaca
     7261 gtcagacggc caccgagaaa ccggtcaccc tggaagaggc tgtcacgctg ctgggaaatc
     7321 gcggcggttc cagtcggatt ctgtcgccga aattctttca gttcgagcag ggttcgcagg
     7381 gcgaagccaa cgtcatcctc tttatcaccg tcatcgctta cggcgcgttg tccgcgggtg
     7441 tcctcaactt gatgcgctcc gacatcatcg gcgccgggat tctggtggtg gtgttcctcg
     7501 gcaacgggct gttgatttca tggctgatcg accagaacgg cgatccgatc gaactgaaca
     7561 aagacgcggt cgaagtgctc ggtgcagtgc agaactgcga aagcgtctac ggtccgcatc
     7621 cggcggtgta cgaggacaac ccgttgagcc cgccgctgac cggctcgtat ctgaaccaga
     7681 tcgtggatgc gcagcgacat ttcggcttct atcaccagaa gttcggtgtg gtccgcgccg
     7741 gccccgcgct ctacggctac tgcggcggaa tcgacatcaa cccgaaccgg ctcgatgacc
     7801 atcggcatgt cgcctatggg ccgtatcacg acatccacgc tcgagtcgag ggcccggcgg
     7861 cgcgcgacgt ggagttgtca ttcgagcagc gttggggccg cgacgggccc gccaacagcc
     7921 agcctgcatt cttcgccccc ggcgcaagcg aactcggtac gcccggcagc gacgccgttc
     7981 aggtggcccg gacgtacttt cgacccgccg attccagccg ggaactggcg tgggctccga
     8041 acggcgatcg caccatcgcc gacacgctcg agcgcgcgat cgatgcggcg cgcgagtaca
     8101 tctacatcga ggagcagtac ttcaccccgg cgccggcgtt ccggcaactg ctagtcgaca
     8161 aggtcgcgtc gcggtccgtc aaggcactca tcgtcacgct gcccgttatc ggagaccaac
     8221 ccttcggcga gatggttcgc tccggtctga tcacggcgtt gcgcgcggcc gacggcggcg
     8281 ccggaatcgt caagatcggt tacccgcgcc gccattacac ggtgcccaac aacaccttgc
     8341 gcacgtcgtc tggccgcctg gtgctgatgg ctgatctcaa tgtcggcgct cagacgatcg
     8401 cgctcgggcc cccgtcccgc attccggtcc cgcctttctg gctcgccgtc gacggcgagt
     8461 acatgtacgt ggtcgacgag tcgatcgcgc ccaacccgga tccggacaac atgctggttt
     8521 tcgaggtcgt tcgcgacgcc gaaaccaggc tcatccgagg tggcgttaca cccgcgggcg
     8581 cacgcgcacg tgcgcacaac gcattcgcgg ccgccacagt cgtctcccac tcgagcatct
     8641 acgtgcacgc caaatcgatc ctcgtcgacg acgtgttcgt gggcatcgga tcggcaaaca
     8701 tcaaccgccg cggccattac cacgacggcg agatgacgct gtacggcgta ccgcagcggc
     8761 tgaaggctga tccggccaat cctgttgcgg cactgcgccg ccgcctgtgg gcggaaatgc
     8821 tcgacatccc acgcgagatt gccgacccgt tgctggcgga cccactcgcc gccgccgcct
     8881 tgttcgaccg ctcgccgctg gcgggtaatc gctacgccga catcgaggcg tatccgacgc
     8941 acctgatgca ggatgtcacc ggtggcgacg ggcttttcct cacacttctc acgacgatcg
     9001 caggcacagc ggtgttgtcg accagtgtgc aggacatcta cgacgggatc gtcgatccga
     9061 cgagcgcgct ggagttcgac tgatggcccc gcaacgaacg accctgttca ccctgttcgg
     9121 cgatcccgca gaacggtggc cgtcagatca cgtccgggag ttcagcggaa cgggtgatcc
     9181 cggcgacccc gccgcctggc tggggcgact gaacaacgct gtgcaagtgg ccaatctgcg
     9241 caccggagcg tcgtccgatg ccgtcgcagc gcaggcagaa ctgagcgtcg acccgtccgc
     9301 aggcttcgcc gccgatcccg atggctggcc gttcgtcatc gcctcgatgc cggacgtgga
     9361 gttccggatc cagccgatgg gcaccaacca gggtcacgtg cagatgttcg cctcacttgg
     9421 gggcaccgga ttcgcgctcg ttctcgaggc cctgcccgtc gagatccgat tgcccgcagg
     9481 actcgtggaa ccgcaccccg accagcccgg tgcgcccgac ggctcgctgg aacaggtgtc
     9541 cggcgagttt cagccgggtc ggctcgacga cctgaagatc gtcctgcggc ggggcggccc
     9601 gacgtcggtg ttcgtccatg tccgcgtcac gatgaccacg tcctacgaat tcgacatcca
     9661 gcctgcggtt ccgatttcct ttggccggtg cggtgtctcc ggcattccgt gtcgggcggc
     9721 tcacgacttc cgattgatcc cgtcgccgga gatcgcgccg aaccacatcg aatggctgcg
     9781 ccaccccgtt gaccccaaac cgtcgccgat caccggcccg tacgacggtc agttcgcggt
     9841 gcgcaccctc gacgtcgacg agaagtcgat gcttgtgcga ccgatggtgg agtggctcaa
     9901 cagccacgcc gacgagaaga cgcccaccgc ggaatgggtc ctcgaggacc tcgtggtgcc
     9961 gttcttcagc ccctacgtga tcccgatacc ggtgcacgcc accaccggaa tccgccgcaa
    10021 ggtcatcgat gtcaccgacc gcggcgatgt ttacgagttc gagcaagatc ccgtgcacct
    10081 cgtgctacgg catgaacaca aactggcgat caacgtcgca tccttcttct accgctcggc
    10141 acctctcgcg gagctcgacg accggttcct gcccaccaag cccgagttcc cgaccacggg
    10201 cttttgggac gaccctgggc tgacattcga tgcggcactg gtgttcggcg acgagcagac
    10261 cccgaaactc aacgacggcg agcctgcgca gcatgcgatt cggttcggcc tcgaggagag
    10321 atggactccc ttcatcggct atcggcgcga atggggcaac gacggacatc cgacggaggg
    10381 caccacccgc tgggagcggt tcgtcaacgt gttgctgcac tgggagatcg ccgaagtggt
    10441 gatcgtcgac atcatgggct tcaagctcgg gctgtcgctc gcgcagtatt tcggcgacga
    10501 tgacacgtca ttcatggaca gcgcgctgct gaccgcggac ctgtacgtgt cgatgccgcc
    10561 gaccggctcc gacacctcga agttccggct gcgcggactg aatggtgaaa aggtcgcctt
    10621 cccgatcgag ggcatcggct ggcggttcgg cagcgtccac ctggaaggtt tagcgctgcc
    10681 cgacggggtg atcttcttcg tctacaagta cggcctgatc ctgtcggaac tcggcgtgcg
    10741 cgccgaggac ggcgccacct acctgacggt ctcgggtggc cttgcggtcg atccccccaa
    10801 gggattcgaa ctcgcggcga cggtcaaggg actgcgcttc cgggtcgcgg gcagcgccga
    10861 tcttccgaag gtcaaggtcg acggcttctt cttgcgcgcc gccagcggaa gcgaatcgtt
    10921 cctcctcgaa ggcggcggct attaccgcga actcgaggac gtcgaagaat tcgcgttcgc
    10981 gctcgcagct cggctgaagt atgcggagaa gaagtactac ttcggcgtcg acggcgtctt
    11041 cggcagccga aaggtcgcgg acgagtcgtt ccgctacttc atggtcgagg cgtactacaa
    11101 gggcgcggta gggcccatcg cggcgtggga cttcaccggc gcacgtgcac tattcgcgcg
    11161 caacatggtg ccgaaactgc gcgacgtcga ccgcgaagcg cgggaactgc gctacttcaa
    11221 gtggtacaag gacaccaacc cgctgaccgt tacggccgac cggcggctga gcgcctggca
    11281 agccagccag gattcgtggg ccctcggcgt cggagcctcg gcgtcgctgg ccggcctcgg
    11341 caaggtgatc gaactcaccg cgttcgtact gatcgtccgc ggccccgcgg agcagggcgt
    11401 gctcgtcgtc gcggaggtgt tcgcgctcgc cagcacgcgg ccgttcggtt acgcggtggt
    11461 cgaggtcgac tcgcacaacg accgcgtcag cgtcctcatc ggcgtagacg tccgcgtcgg
    11521 caccttcgtc aaggacgcgc ccgcgtggat gaacgacgtc ggcgcggtga ccgggacgtt
    11581 gttcatttcc aacgacccgt gcacgttcgc cattggccgc cttgccgatt cggccacctg
    11641 gctgggtctg cgattcgagg tagacctgtg gctggaggca tcgctggtca tcgcgttctg
    11701 tgtcgagcac gtcgaaggcg gccccaacgg cttcgggctg accgcgcgca tcgagggcgg
    11761 catcggcaag aagggcgtcg tccggttgtc ctacaacgcc gggttcacgc tagtgatgac
    11821 cagtttctca acgggttcca ccgattacgc tctcgttctc gagatcggcg cgggcatccg
    11881 gttcgagctg ttcggcttcc tgcgcatcgg cgttagtacc cgcatggcgt ttcgtatcgt
    11941 cggcggtcat cccgcccgcg ccgaattgac cgccgagatc cgtttggaga caccgtggtt
    12001 cctgccggat gtcacctggc gtctcgacgc ggtgtacggc gacctgcggc ccgccgatct
    12061 gtccagtgcc gtgcagccga ttcgcagtgc ttccgccaag gaactcaccg gcacacagct
    12121 cggcgtacac ctcgagagat tcgacccggc gtggaacggc gaaggcgttg cgccggtgca
    12181 cagcgtgaac gagctgtccg ccgcgccccg gccggaagtg cagcggctga cgaacttcga
    12241 caacgacgcc gccgcaaggc ccgtggcgac cgacgcgaca atcgccgtca catgggcggt
    12301 ggccgtgaac gacaggatcg ggctcggcac gggggtcgcc ggggctcttg gcacccaaga
    12361 ggcaggcgac ctgatactca cctatgacct cgtcgccctc gcagttcggc ggcgcccgcg
    12421 gttcggcctg caacgcgatt ggagcccgct ggagagcaag gtcgaactag gcgtggactt
    12481 cagcaatccc gccgggccac agctcagtgg gacgttcgcg cctcaggtcc tcaacaagtc
    12541 atgggatctc gacgtcatca tcaacggcga gcccgcgccg aagcgactgc tgctgaactc
    12601 cgttgcaccg tacgacttca cattcttcag tccccaggcc gacgaccagc tgactcagca
    12661 tcatccgagc tggccctgct gcgaccacat cgacgacaag gatctctccg acctgttcca
    12721 ccgcgtccac tggcacgatg cggggctcgg tgagcccctt gacgccccgg ccacaagccg
    12781 attcagcgac agcaacagca ctctgcgatt cctgaggccg gcatggactc gcccagcagt
    12841 gttcgggggc ctcgtcaacc cgaccaccgt cgcggccgcg cggctggggc agccgggcgt
    12901 cgtggcgcgg gccgatttcg atgaagatgc cgcactgtgc agtgtccgga ttgcgtctcc
    12961 acgcgccacc cgggtgacgg tggtgctgtt caaccggacc ggcaaagagg ttggccgaca
    13021 agagctggcg gtcaccgcag gcaccttcca gaccgcggta gtcgccgcga gcggtccggt
    13081 gacccgcctg gagatccggg cgaatcctgc cttggccacc acggcgcctt gggctgcggc
    13141 atcggcaccc gctgacgccg tgatcgaggt cgacgaggtc gcatacgtgg gcctgcgcga
    13201 ctatctcgac atcctcatcg ccgaaaccgc ctgtggtggt ggcccgatgg gcggcggctc
    13261 aggcaagctg gcctttctgc ccaactacga gtacgaggtg tcgctgacga cccgggtgat
    13321 cgtcgcgcat ccgtcagcgc ccgaagtgac cgccgatgtc gaggagttcg tgtatttcaa
    13381 gaccaagggc atgccgggtc tcaacgcggt caatcgcatc ggtgaggaga tcgaccccta
    13441 cgtgcgcgcg gcctacacag gtgggcatgc gggcgtggtt taccgggaag agcctgtgac
    13501 gcttgcattt tcggaaggct ttcacgtcgc ggtccccatt tcggtccggc cgcccggcac
    13561 ggtggccgag cacactgaac ttatgcgcat gcaactgctg gtctccccgg accaagcgaa
    13621 ccgcgccgat accgcttata ccgtgacgtc gcccgactgg atcgtcacgc atcctggcgc
    13681 tggcacgccg ccgcgcagcg gggaccatgt ctggacaccg gtcgtcagtg gctcacgcac
    13741 gctggggagt tcgatgcgct cggccgatcc gatcaccgtg cggttcgccg cgatggcaca
    13801 gcggcccgca gtcacatgcg gcgtacccga tccttcgcag gtgacgggga ctgtgctgat
    13861 tgccgaaccg cccgaccccg acacgggtct gtgggagccg ggcatgacgt acacggcctc
    13921 ggtgcgcaga gaaagtgcac cgttcgtcga tcgtcgcccg ttcgaaccgg gcgacgagac
    13981 tgctctcacg gcgggaactc tcggctcggg cagcgcggtc tggacggtga tggacgggca
    14041 cctcgaccgg gccggcgatg gctgggggtt cgcgaccttc ggcgacgcca cttgggaaca
    14101 tgccacggtt ctggtcggca tcgagccagg cgccgcggcg gcgggcttcg gcgtcggcat
    14161 cggcggcagc gccgggctgt tcgcactcgt tgaagagagc gcaaacacgc gacgtttggt
    14221 tatcgggcga cgccaggcaa cggcgctgaa cgaggttgcg ggcgcggacc tgccggacgg
    14281 ccccgatgcc gccatactgc tacaagtcac ggctttcgac gatcgactgc gagccacagt
    14341 gggcgacact gtcgtcgaag ctgaccgcgg cgaggatcgg gcaggccgca tgtgcctgtg
    14401 cagcgacggt cctgcctcct tcgcatcact tcaggtgaac ggcgtggaca tgtatcaatt
    14461 ccgtttcacg gcaagcaggt tcacgtcttt cgaagagcac attggctcat ggtcaggacg
    14521 cctcgacgac ctcggccccg atttactggg gccgggtacg actacggcaa cagttgctca
    14581 actatgggcg acgtcatctg gtgacgtgac caaggtgatg gatccgggcg cggcacccgc
    14641 agatcgagag cgtgttttcg ccgggtggac cgcgggactt ggactggcga cgagagacga
    14701 cgtcacggct ctgggactca gccgctacgt cgaagaggcg cagacccgcg ctctgctcat
    14761 agagtcaccc gaaccgatgg acttcaccgc cgacatcgcg gtggcgctga tcggccgaca
    14821 ggtgcggcat ccgcggcctc cacgcccgag gcctccgatc ttcgatgagc gcacgctcgc
    14881 ccaccggctc acgaccattg cacggcaacc ggaactcccg ctgccggtcc gcggtggccg
    14941 gccgtcgatc gccgacgcga tcatcgatgt cgaggtcgac cgggcctcgc tgcgattgca
    15001 gctgcattcc tcactcgcca acgcaggtga ggtcgccgtc gtggtcacga ccgcagacgg
    15061 cgccctggac ctgtaccgcg ggatcgtccg gccgccatgg ttgcgcggca ggccggccgt
    15121 tctcgtggcc gcgctcgtcg gtccactggg ccacatcccc ccgggaagtg acctggccgc
    15181 tgagctgcag gcggcccggc ccggcgcgct gttcctgctg acttccgacc tgatcgagct
    15241 catcgggcgc tgggacatgc tgcctgtcga ggtggatgtg ccggtcgatg ttcgcgtgtt
    15301 gcaaaacggc gatgcccgac gcgccctcgt gatacccatc accggcggcg cggcatccgt
    15361 actgccggtc ggcccctatc ggctgacgct gtcgctggcg cgcaagcgat gggagactac
    15421 agatcccacc gatgacatca acagctacca acggtcggtc accgtccgtt tcgacgtcac
    15481 cgcggattaa gcgctgaaca cgtagtcgtc gagcgggaag cggcggctgc gccagtacgt
    15541 ctcggcggtg gtggcaggcc gtagtggcac gtcgccgttc ttgtcgaagt agtaactgtt
    15601 ggccagctgg cagctgtcct gccagaagat ctgccggtgc cgcttgcgca tcatctcggc
    15661 gaagtagcgg tcgttggcct ccttggtcac ctcgacacgg gtggcgttgc ggcggcgggc
    15721 ccgcttcagg cagcgcacga tgtggtgcgt ctgcgtctcg atcagcgcga agtacgacga
    15781 tccgacgtag ccgtacggcc cgaacactgt gaagaagttc gggaagccgg gcacgctcac
    15841 gccctcgtag gcctgcagac gattctcgtc ccagtgctgg ctccacgacc ggccgccggg
    15901 cccggtgagc ggataggtcg gcatatcgtc ggtgtccatc accttgaatc cggtggccag
    15961 gatcagcaca tcaacgtcgc gcgtctcgcc gtcgacggtc gcaacgccgg aaccggtgat
    16021 cttgtcgatc ggctccgtca ccaactccac gttgtcgcgg ttgaacgtcg agaggtaggt
    16081 gttgtggaac ccaggccgct tgcagccgac ggcgtagcgc ggcgtgagct tgtcccgcac
    16141 ctgcggatcg ttgacctgct tgcgcagata cgcctcgccg accttcgaca tcttcttggc
    16201 catcgggttg acggtgaagt actgcgccgg cagagcgaag gtgagctcga cgtacgcctg
    16261 actcagaatc cgctgcaccg tgctgccgcc cggaagccgc atcatgcgcc gggccatcgg
    16321 cgacagcgga acatcgggct tggggaagca ccagattggt gtgcgctgaa agacggtgag
    16381 ttgctcgaca attggcgcga tctcggggat cacttggact gctgaggcgc ccgtcccgat
    16441 gatcgcgacg cgcttgccgg tcaggtcctg gttgtggtcc caccgcgcgg tgtgcatggt
    16501 caggccggcg aacgagtcga cgccgtcgat gtcgggcagc ttcggtgtga tgagcacacc
    16561 gctggcgttg acgaggaatc gtgcggtgac cacgccgccg ggatcggtgc ggatgtgcca
    16621 caggctttgt ttgtcgtcga agtcggcgcc gagcaccttg gtcttgaacc ggatcttgcg
    16681 ccgcaggccg tacttgtcga cgcagtgttc cgcgtaggcc ttcagctcct tgcctggcgc
    16741 gtaggtgcgc gtccaattcg ggctcttctc gaacgagaac tggtaggaaa acgacggaat
    16801 atcaacggcg ataccgggat acgtgttcca gtaccaggtg ccgccgggcc cgtcacccgc
    16861 ctcgatcacc agatagttgc cgagccccga cttgtcgagc ttgatggcgg tgccgatgcc
    16921 ggagaagccg gcgccgacga tcacggtgtc gaagtcgggc actgtcttca tctatcagtg
    16981 ctcctctcgt ccgcgggccc ctcccgctga tgggcgatga cgtgcagtcc gtgcttgggc
    17041 cgcaacgtca gcgttgcttc gagttctacc tgaaagtcgg gcaccaggtc aaaggtgaag
    17101 cgctggctca tgattgcggc catcagcacc atctccatca gcgcgaagct ctggccgatg
    17161 cagatccggc gaccgccgcc gaacggcagg tatgcagatc gtggccggtt cttgcctgca
    17221 tcacccaaaa accttgtcgg gtcgaatagc tcgggatcgg gccaccagcg ctcgtcgtga
    17281 tggatgtggt ggatcggaat cagcacggtc gtgtgcggac gaatgtgatg gccgtcgatg
    17341 aggtcgtcct cgaccgcctc acgagcgatg atccagaccg ccgagtaata gcgctgcgat
    17401 tcctgcaggc atgccgtggt ccatggcagc agcgtcaggt ctgcagcggt tggacggcgg
    17461 ccgccgagca cgccgtcgat ctcggcgagc atcttgtctc gcgcgtcggg gtgctgcgcc
    17521 atcaggtaga aaaaccacga catcgcgttg gcggtggtct cgtgaccggc gagcatgaat
    17581 gtcaacgcct cgtcgcggac tcgctggcgt ggccacgtcc cgttgtcggc cttgaacaac
    17641 acgttgagca ggtcggcgga gtcggtcggg cgggcgatcc gctcctcgag cacgccgttc
    17701 accgctgcgt cgagcgccaa tgcgatcttc tggccttccc gcaggggcgg cgggagcggc
    17761 acgcctgaga acgtgctcca ggagagcgcc tggtagatgg gccgcggcag caggccccac
    17821 aggccgagtc tgccgagtcg ctcggtgcgg cgaaggccgc gtttcgtcag gtccttcatg
    17881 ctgtgcacga gggggccgaa atcctgactg aacagggcgt tcgccaccac ccgtagggtg
    17941 gcttcgacca tcgtcgcgtg catgtcgaac tcgacgtctg gcacgacctc ggcggtaacg
    18001 cggtcgatcg ggtcgatcat cagatcgacc agctcgttga ggtgtcgccg cgcgaaggtg
    18061 ggattcagtg tggttcggtg ggcggcccac gaatcgcctt cgtcggtgag caggttgatg
    18121 cccgcagtgg cgcggatcgg ctcgtagtcg ttggacttga cgtacttcag ccgagcccgg
    18181 tgcagcacgt ggtcgacgta atctggatgg gtaatagaga cgaaacgcct tcccgcgcaa
    18241 cggaatcggg cgatatcatg gccgcgcgta cggccgagga agcctccgcc tgcatcgaat
    18301 ccgatggtga tggcctcgcg tgacatcgtc aatgtgctga gccgcgccgc cggagccgcc
    18361 atgaatcaac ggtatggctg acagaccgga cccggaatgg gagtatcgga cacagagttg
    18421 ggagattggg acaccggcat ggcttcgacg acgtggtcac ccgacctgcg gcacccgatg
    18481 tatgcgacca ggctgttgtg cgaggtcgcc aacgagcgcg gagtgccgac ggccgacgtg
    18541 ctgaccggca cagggatctg tgctgacgac ttggacgagc ccgaggcggt catcaccgcg
    18601 tgggacgaaa tcgtcgctgt gcgccggctg ctggcccggc tgcccgacga tgtcggcgtc
    18661 ggcgtcgacg tgggcagccg attcaaactc acccatgccg ggctgctcgg cttcgccgca
    18721 atgtcctgcg ccacgctgcg agaaatgttc gacatcgata tgcgttactt ctcgctcacg
    18781 atgctgcaca tcgacctcaa gctattcgac ggcgcgcagg actgcgtgct cgaactcgac
    18841 gtcgaccact tgcccgcaga tgtgcgccga ttcttcatcg agcgtgacct tgcggcgatc
    18901 gtttcgacga taggggcctt cgcatacgaa gtcgtcgagc gctacgcgga caaagtcacc
    18961 gcggaggtat cgctcgacaa ggacgtgctg agtccgctgc tgacgttggt gccggtcgaa
    19021 aacatctcat tcgagcgcgc acacacccgg ctgcatttcc cgcgcgccat gtttgacgag
    19081 ccattgcctc aggctgatgc ccacacgctc gaactgtgca tggcacagtg cgatgtgctg
    19141 atgcagcgca acgaacgacg tcgcggcatc acagctgtcg tgcgatccaa gctatttcgc
    19201 aagtccggcg ggtttccgac actgcccgag gtggccgccg agctcgacgt gcacccgcga
    19261 acgctgcgac gtcagctcgc ggaggagggc acctcgtttc gcgcgctgct caacgaagca
    19321 cgctcgacgt tggcggtcga cctgctctgc aacgtcggac tcaatgtcga ggaagtgtca
    19381 aagcggttgg gctacacgga cacctcgacg ttctgccacg cgttcaaacg gtggcacgga
    19441 gtcccgccga gcgcttatcc gcgggcctag gacgtcggcc agcgaccgtc ggctttcgcg
    19501 gcgtcgatct gagcctgtcc tggcgcgcgc atgcggccgg catcgatgag ccgttgcacc
    19561 cgctcgacat tggatttcga ccatgcgctg cgtgatttcc gtgggctgaa ccgctggata
    19621 cgcgaaatgt cgtcgtggcc cctcgcgagg ctgtcgatcc agccgaagca cagcgcgatc
    19681 tccaccgcct cgccgtacga cataccgggc tccggcgcgt tcttgttcat cagccgcagc
    19741 cagatctcgt cggccgcttc gtgattgcgc tccagccacg cttcccattg ctgggctgtg
    19801 cgcgcgtcga gcacctgtcg ttcggtcatc cgcggtgcag tgcggccttc agcgccgcca
    19861 ggccgcggtt ggtggcttcg gccgcggcgg gagcgaccag cgcgaagtta acgtagccgt
    19921 gcaccagggt cggcgcgttg tgcagctcca cggggacccc tgcggcggag agcaattctg
    19981 cgtagcgggc accgtcgtcg cgcagtgggt cgtattcggc ggtgccgatg taggccggcg
    20041 gcaggccgga caggtcggca gtgttggccg gcgcgagtgt gggtggcagg ttggccgcgt
    20101 cggtccagtc gatctcgggc ggcagatacc actgctggaa cgcggcgacc acgtcgcggt
    20161 ccaggatcgg cccgtcggcg ttctcgatca tcgacggcag cgtcatgtcg ccggtcgtcg
    20221 acggatacca gagcagctgg aagaccagcg cgggcccgcc gttgtcgcgg gccagctgcg
    20281 ccatcaccgc cgagatgttg ccgcccgccg agtcgcctgc gaccgcgatg cgtgcggggt
    20341 cgccgccgag ctcggaggca tgctcaccga cccactgcag cgccgcccac gaatcgtcga
    20401 tgccttgcgg gtacgggtgc tcgggcgcca gtcggtagtc gaccgacacg acgatggctt
    20461 cggcgccgac ggcgtgggcg cgggccaccg ggtcatgcgt gtccaggtcg ccgatcgccc
    20521 agccgccgcc gtggtaaaaa acgacgaccg gcaggttgtc gtgctgtgcg atcggcggcc
    20581 agtagatacg cacggggatg ccggtgatgt ccccgtagtc gatggtgcgt tcctcgatcc
    20641 gcatgtcggg caacgcttcc ggcggggcct tcagctgccg catctgctca cgagccacct
    20701 cgacgccctc gtcggcggtg aacttcagcg ggaatgcgtc cagcagtgcc tgcatggtcg
    20761 gatcgatgcc gggccgcgcg gtcggggatt cagccatggc cacacggtag cccccgcgtg
    20821 gcggcctact caggccagcc gctgttgagg atgatgtcga cgaagtcgtc gcggacgaac
    20881 gtcgggtcga aatgctcgag cacgtcggcg ttgatggtcc cgtaggtgct gtgcggccgg
    20941 tgcaggttgc cgtcggcgaa cgcctgcagg atgcgacgct tgaaatccgg tcggggatgg
    21001 gccgtggtga tggcgtcgcg tacctcgtcg ggcaggtcgt cgcggccgac gccgacgacg
    21061 tccgtcttga caccagcgtt gacaagcgcg atttccgggt cgaggaactc tggaacgcca
    21121 ggcgtggtgt gcagcgcgat cgacagccag accctgcgca ccgctgcgtc gtctaggccc
    21181 cggtcgcgca agaacttctc cgccgcgttg gccccgtcga cctcaaagcg aagcattgaa
    21241 tcgcggaagt ccgccgtcag gccgatgtcg tggaacatcg cgccgacgta cagcaattcg
    21301 aggtcggggt cgaggccgcg tcgttgcccc tgcaatgcgc cgaacaggaa cacacgtcgg
    21361 gagtgatcga agagcagatc atcctcgacg tctcggatgt actcggtggt gtctcgtacg
    21421 agctgtgtgt ctggaatcgc gattcccgcg atggtttgtg cagtcatggc ctcagtgtcg
    21481 gcgcgtcggg cgcatgagcg gccgtgtccg ttgtgccatc tttcccacag aaagcgacat
    21541 agggtgggtg catggccgcc gatcgcgtca tcgtgtttgt ggtgttcgac ggcatgacgc
    21601 tgctcgacgt cgccggtccg gctgaggtgt tcgccgaagc gaaccggttc ggcgcgtcgt
    21661 accggttgtt gatggcgtcg gtcgacggca aggatgtggt gacgtcggtc ggcgcgcggc
    21721 tgaccgcctc gacgtccatc ggttcggtca aagcggtgga caccgcggtg atttcgggcg
    21781 gtgaccatct ggtcagacgc ccgatagcgc cagatctggt cgccgctgtc aaggacctgc
    21841 ggccgcggac gcgccggatg gcgtcgatct gcaccgggtc gttcatcttg gccaagaccg
    21901 gaatcctcga cggccgtcgc gcgaccacgc attggcggca agcagggctg ttgtcgcgcg
    21961 cgtacccgca gatcagcgtc gagcccgatg cgatcttcgt gcgcgacggc gacatctaca
    22021 cctcggccgg ggtatcggcg ggcatcgacc tcgcgttggc actcgtcgaa gacgattacg
    22081 gcgcggacct tgttcgcgat gtcgcccgct cgctggtcgt gtatctgaag cgggcgggcg
    22141 gacagtcgca gttctccgcg ctggtcgaaa cgcctccgcc gcagcggttg cacttgcgtg
    22201 ccctgaccga ggccatcgcc gccgatccca gtgccaacca cacggtgaaa acgcttgcgg
    22261 cgcaggcatc tctgagcact cggcaactga cgcggttgtt tcaaagcgaa ctcggaacca
    22321 ctccggcccg ctatgtcgac atgatccgaa tcgacgccgc ccgttcggcg ctcgatgccg
    22381 gccgctcggt cgtggactcg gcacgggtcg ccggtttcgg aagcgccgag acactgcgcc
    22441 gggtgttcgt caaccagctc ggtatctcgc ccaaggccta ccgcgaccga ttcaagtcga
    22501 ccgggtgagc cgctgcagct gctccagcgc ggcacgctcc acgtggttgc tgagcagttt
    22561 tccgtcctcg cggacggccc agaagtcgac gttctggaac gattcgggga tctccagatg
    22621 caagtcgacc acaaaccggt ccaccgccgc ggggtcgtgc gcgttccagc attcctcccg
    22681 gaaggcgtgg acgatgtcga cggaatcact catggcgcgt gcaatgctgc gcgcagcgcg
    22741 gaaagtcccc ggtccatcgc atcggtcgcg gcgggcacta cgccggcgta accgaggtag
    22801 ccatggatca gcgtctcggc gttgtgcagt tcgacgggca cacccgctgc ggcgagcagt
    22861 tcggcgtagc gggcgccgtc gtcgcgcagc gggtcgtggc ccgcgacggc gatatacgcc
    22921 ggggcaaggc ccgccaggtc gcccgctcgt gccggtgcca gcgtcgcagg catgtccgcg
    22981 aggtccaaat cgcccacata ccaacgggaa aaccccttga ccgacgcgtt gtcgagcagc
    23041 ggggcttcgg cgttctcggt gaacgacggc agcgacgtgt cccacgtcgt cgccggatac
    23101 cacagaagct ggaaccggat cggcggtccg ccggcgtcgc gcgccagctg cgccatcact
    23161 gccgcgagat tgccgcccgc cgagtctccc gcgacagcaa gtcggtcggg atcagcgccc
    23221 agctcatcgg cgtgcgcggc cacccattgc gttgtggccc aagcgtcttc gaccgcggcg
    23281 gggtaggggt gttcgggcgc gaggcggtag tccaccgaca ccaccaccgc gccggcaccg
    23341 accgcgtggt ggcgtgcgtc gccgtcgtgg gtgtcgaggt cgccgaccga ccatccgccg
    23401 ccatgcagga agacgaccac ggggagcgtc gcctcgccgt ccgtcggcgg ccagtacacc
    23461 cggatcggga tcggccccgc gggtccgtcg atgctgcggt tttcggtgcg cacgtcggga
    23521 tgaaactgcc gacgcggcag ggcgcggaac cgttgccgcg cggcctcgac acctccgtcg
    23581 gttgtcaact gaaacggaac cgcctccagt accttcagca ggatggcgtc gacggcgggt
    23641 ttctcctcag caacggacat ggggacaccg tacgtacaag cgcggacaag gctgctctgg
    23701 gtggccgcgg cgatcgtcgg cgcgtgctac ggggtttttc tgatcgtcac ggcgctgcgg
    23761 ttgccggccg gcgccgaact caccggccag ttcacgctgc agccgtcggt caaggcgttg
    23821 acggcggtgc tgttggctgt cgccgcgctg acacacccga tcccgcggga acgccgatgg
    23881 ctgatcgcgg ccctgctgtt ctccgctgcc ggcgactttc tgctcgcgat gccgtggtgg
    23941 caaccgtcgt tcgtgctggg attgggcgcc tttctggtcg cgcatctgtg cttcttggcg
    24001 gcgttgatcc ctctggtttc gcggtcgggg ccgcggctgg tggccgccgc ggtgatggtg
    24061 ttggcctgcg tggtgctact ggtgtggttc tggccccgac tgctcgccga ggagatggcc
    24121 gtgccggtga cgctctacat cgcggtgctc ggtgcgatgg tgtgcgccgc gttgctggcc
    24181 cggctgccga cgccgtggac ggcgcttggt gcggtgtgct tcgcggtgtc cgacgggatg
    24241 atcggcatcg gcaagttcgt gttgggttcc gaggcgttgg ccgtgccgat ctggtgggcg
    24301 tacgccgcgt cgatcttgct catcacggcc gggttcttct tcggccgggc gtctgtgtcc
    24361 tctgctagac cttctgcgtg accaccaacc gccagcaggc cgaacacgag cccatcgcgc
    24421 gtgttgtgcc catgctgacg gtgcctcatc tggatcgcga attcgactat ctggtgtcgg
    24481 ccgagcaatc cgacgatgcc cagcccggcg tacgcgttcg ggtgcgcttc cacggcaggc
    24541 tggtcgacgc gttcatcctc gaaaggcgtt cggacacaga ccatgtcggc aagctcggct
    24601 ggctcgaacg tgtcgtgtcg gctgagccgg tgctgacgcc cgacgtgcgt cgcctcgtcg
    24661 acgccgtggc ggcgcgttac gcgggtaccc gcgccgatgt gttgcggctc gcggtcccgc
    24721 cgcggcacgc gaacgtggag aagcaaaccg ccccagagct cgcgccactg accggcaaac
    24781 ccgtcgacac cgccgcctgg tcggcctatg ggcgcggcga gcagttcctt gccgccctgg
    24841 aggacggccg cgccgcccgg gcggtctggc aggccctgcc cggcgagcac tggacggacc
    24901 ggctggccga ggctgccgcc gtcaccgtga actccggccg cggagtgttg gccatcgtgc
    24961 ccgaccagcg tgacgttgat gcgttgtacg ccagcgcaat tcgctatgtc gaagcggaca
    25021 gggtggccgc gctgtccgcc ggactcggac ccgcacagcg ctaccgccgc tggctgtctg
    25081 cgctgcgcgg gggtgcacgc atggtcatcg gtacccgcag cgccgtgttc gcgcccgtgg
    25141 ccgacctcgg cctggtcacg gtgtgggacg acggcgacga caccctcgcc gaaccgcgcg
    25201 ctccctaccc gcacgcccgc gaggtggcga tgctgcgcgc ccaccaactg cgctgcgccg
    25261 cactgatcgg cggctacgcc cgcaccgccg aggcgcaggc gctcgtccgc agccggtggg
    25321 cgcatgacct cgtcgcggcc cgtcccgtcg tacgcgaccg ctcgcctcgc gtcgtcgcgc
    25381 tcgacgacag cggccacgaa caggagcgcg accccgccgc ccgtaccgcc cggctgccgt
    25441 cgctggcgct gcgggccgcg cggaccgcct tgcaggccgg tcttccggtg ctggttcagg
    25501 tgccccggcg cggctatgtg cctgccctgg cgtgtgcgcg ctgccgcacg atcgcgcgct
    25561 gcaggcgctg caccggaccg ctttcgctgc cggaccgcga caccgcaggg gcagtgtgtc
    25621 gatggtgtgg ccgtgaggag cccgcgttgc gttgcacgcg ctgtggctcc gacgccgtgc
    25681 gcgcggtcat cgtcggtgcc cggcgcaccg ccgaggagct cggcaaggcg tttcccgaca
    25741 cgcctgtgat cacctccggc ggtgaggcga tggtcgccgc ggtgcccggc gagccgtccg
    25801 tcgtcgtcgc gacacccggc gccgaacc
//
LOCUS       DASRJA010000202         7657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2688354,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000202 DASRJA010000000
VERSION     DASRJA010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2688354"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..658)
                     /locus_tag="VFC01_09090"
     CDS             complement(116..658)
                     /locus_tag="VFC01_09090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZN79825.1"
                     /translation="MSDIKVLVLLGSLRAASINRQLAELAVEAAPEGVSLQLFDRLGE
                     LPFYNEDIDNDDVAEPVVALRAAAAEADAALVVTPEYNGSIPGVLKNAIDWLSRPFGN
                     GALKGKPVAVVGAAHGRYGGVWAHDETRKSFGIAGPRVVEDLKLSVQTTAFDGKHPRE
                     NAEVAATLRDIVGKLAAEVA"
     gene            749..1357
                     /locus_tag="VFC01_09095"
     CDS             749..1357
                     /locus_tag="VFC01_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79826.1"
                     /translation="MTASPPLRELPVTDQAPQERGDAARNRTLLIDAARRLIAERGAD
                     AVTTDDIAAAAGVGKGTLFRRFGSRAGLMIVLLDEDEKVEQQAFMFGPPPLGPGAPPL
                     ERLLAYGRDRLHFVQAHYALLSDANRDPQMRFNDPMMLHHHHIRVLLEDAGTTGDLDA
                     QAFALHSLLDADYVQFQLTERGQTLETLGDAWESVARKLCGI"
     gene            1354..2394
                     /locus_tag="VFC01_09100"
     CDS             1354..2394
                     /locus_tag="VFC01_09100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZN79827.1"
                     /translation="MTPTWVLHVDLDQFQAAVEVRRRPELAGLPIIVGGNGDPNEPRK
                     VVTCASYPARKFGVHAGMPLRTAARKCPDATFLPLDTDAYDAASEEVMDLLRDLGHPV
                     EVWGWDEAYVGANVDAPVRLAEQIREVIGQTELSCSVGISDNKQRAKVATGFGKPAGI
                     YQLTDENWMAVMGDREVDALWGVGPKTAKKLAGMGITTVADLAATDATLLTSTFGPTT
                     GLWILLLAKGGGDTNVSAEPWVPRSRSHVITFAKDLTDRTEIDSAVVELAQQTLKEIV
                     AQRRIVTRVAVTVRTNTFYTRTKIRKLPAPSTDADLINKTALDLLGLFELARPIRLLG
                     VRLELNAPEGGY"
     gene            2401..3585
                     /locus_tag="VFC01_09105"
     CDS             2401..3585
                     /locus_tag="VFC01_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN79828.1"
                     /translation="MLHTVAIRGYRSLREIVLPLARLTVVTGANGTGKSSLYRALRLL
                     ADCGRGEVIGSLAREGGLDSVLWAGPEHLSGARRTGKVEGTTRTGPVSLELGFASDDF
                     GYLVDLGLPQMAGHNSLFALDPEIKREAVFAGRVMRTSSTLVRRTRDYAEASAESGRG
                     FDEMTRSLPLYRSVLAEYAHPGAHPELAAVRHRLRSWRFYDGFRVDMAAPSRLPHVGT
                     RTPVLSDDGGDLAAAVQTIIEAGFDDLQRAVADAFDGATLSVAVHDGLFDLQLQQRGM
                     LRPLRAAELSDGTLRFLLWAAALLSPQPPSLMVLNEPETSLHPDLVRPLASLIQAAAG
                     QTQVVVVTHSRAMLEFLDTVPITDAEQGGAVEVELYKELGETRVKGLGLLTTPPWDWG
                     KR"
     gene            complement(3582..4442)
                     /locus_tag="VFC01_09110"
     CDS             complement(3582..4442)
                     /locus_tag="VFC01_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN79829.1"
                     /translation="MAQGSGFSGKRCFITGAASGIGRATALKLAADGAELYLTDRDAD
                     GLEKTVLDAEALGATVAEYRALDISNYDEVAAFAVDIHTRHPAMDVVMNIAGVSAWGT
                     VEQLTHQHWRSMIDINLMGPIHVIETFVPPMVAAGRGGHLVNVSSAAGLVALPWHAAY
                     SASKYGLRGLSEVLRFDLDRHRIGVSVVAPGAVKTPLVQSVQIAGVDRDDPRVQKWTN
                     RFSGHAVSPEHVADRILGGVRKNRFLIYTSPDIRALYLFKRTAWWPYSIAMKRVNVFF
                     TRVMRPARRG"
     gene            complement(4465..5085)
                     /locus_tag="VFC01_09115"
     CDS             complement(4465..5085)
                     /locus_tag="VFC01_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN79830.1"
                     /translation="MTAQPAPQQIRRSRGDRQREAIVNAVRELLQERSFADLSVSTIS
                     ERAGVARSGFYFYFDSKYAVLAVILADAGDLLDNLTDHFAPREPGETPAAFAKRMVGS
                     AAAVYANDDPVLKACAVARNTDAQIREMMDDFYDGIIDKLITLLEQDSDARPISDDLP
                     ALVRTLAAVTSFTLTQDSAFVGRGEDPARAVDIVERLWLNALWGDE"
     gene            5205..6686
                     /locus_tag="VFC01_09120"
     CDS             5205..6686
                     /locus_tag="VFC01_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN79831.1"
                     /translation="MPTISTKDYLLDQARRRLKPTAVTIPGMGLAEKRLRKHEFKQFP
                     LAEPPTGSGLKPAMGDSGLPIIGHMIEFFRMGPDFAVEIYRKYGPVHYQYSPALSSVM
                     ALGPEATQAVFSNRNKEFSQRAWDPVIGPFFDRGLMLLDFDEHMYHRRIMQEAFTRTR
                     LSGYVEHIDSVASHVVANDWVANDPRFLFHPAAKELTLDIASVVFMGHEPGTDRELVT
                     TVNQAFTTTTRAGNAIVRKPVRPLTWWRGIQARKTLEDYFAARLTETRGSEGTDMLSV
                     LRQAEDEDGQRFTDADIVSHMIFLMMAAHDTSTSTMTTMAYHLAANPDWQERCRDESA
                     RIGDGPLDIEALEKLETYDLVINEALRMVTPLPMNFRRAVRDIDLLGYFIPAGTDVVT
                     WPSLNHRLPELWTDPEKFDPDRFAEPRSEHKKHRYAFAPFGGGAHKCIGMVFGQLEIK
                     TVMHRLLRKYRLELVRPDYKPRYDYGGMPVPVDGMPIVLRPLR"
     gene            complement(6690..7181)
                     /locus_tag="VFC01_09125"
     CDS             complement(6690..7181)
                     /locus_tag="VFC01_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HZN79832.1"
                     /translation="MRNTSATSAAPLTFAAHVDGPMPRGLRQEADAMSFEAFHDEYAS
                     SPGPLRLGNWQCTDTHRPSTRLGPQARNYQATLAFGDRISTSSAAACGPVAALTEMLY
                     DRGIAVEMTAFHQVPAGEHTATFVRGSDGARSEWAMGWSDDPTQSALRAVIACANRLL
                     TEA"
     gene            7452..>7657
                     /locus_tag="VFC01_09130"
     CDS             7452..>7657
                     /locus_tag="VFC01_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination regulator"
                     /protein_id="HZN79833.1"
                     /translation="MNTQTPRTRDTAIPVGDRVGSFRYLRAEDRWEWSGAVAQMHGYA
                     PGQVHPTTALVMSHKHPDDAANVAR"
BASE COUNT         1331 a         2579 c         2436 g         1311 t
ORIGIN      
        1 atcttcatcg acggtgccct attgcacgcg ctcatcacgg gtgaaccgtt gagcaccgca
       61 gcccttgcag aggctctcgc caagctgctc gcgccttagc ccgaatgctt ggtgctcagg
      121 cgacttcggc agccagcttg cccacgatgt cgcgcagcgt cgcggccacc tcggcgttct
      181 cacgcggatg cttgccgtcg aacgccgtcg tctgcaccga cagtttgaga tcctcgacca
      241 cccgcggtcc ggcgatgccg aacgacttgc gcgtctcgtc gtgcgcccac acgccgccgt
      301 accggccatg cgccgcaccg accacggcga cgggcttgcc tttcagcgcg ccgttgccaa
      361 acgggcgcga caaccagtcg atcgcgtttt tcagcacgcc ggggatgctg ccgttgtact
      421 cgggggtgac gaccagggcg gcgtcggcct ccgcggccgc ggcacgcagc gcaaccacag
      481 gctcggcgac gtcatcgttg tcgatgtcct cgttgtagaa gggcagctcg cccagccggt
      541 cgaacagctg caggctcacc ccttcaggtg ccgcctcgac ggccagctca gccagctggc
      601 ggttgatgga cgccgcgcgc aggcttccca gcagcaccaa taccttgatg tctgacatcg
      661 tcacgtttcc cttcggtggt ctgatcgatc ttcgcacgac ccaaccggac cgtggtccga
      721 tttattccgg ctgaactaaa ctgcagctat gaccgcatct cccccgctgc gcgagttgcc
      781 ggtcacggat caagcgcctc aggagcgggg cgacgctgcc cgtaaccgca cgctactgat
      841 agacgctgcg cgtcgcctga tagccgaacg cggcgccgac gccgtcacga ccgatgacat
      901 cgccgcggct gccggagtgg gcaagggcac gttgttccgc cgattcggca gccgagccgg
      961 cctgatgatc gtgctgctcg acgaggacga gaaagtcgag cagcaggcct tcatgttcgg
     1021 gccgccgcca ctggggccgg gtgcgccccc tctggagcgc ctccttgcct acggtcgcga
     1081 ccggttgcac ttcgtgcaag cccactacgc gctgctctcg gacgccaacc gcgatccaca
     1141 gatgcgattc aacgatccaa tgatgctgca ccaccaccac attcgcgtct tgctggaaga
     1201 cgcggggacc accggcgacc tcgatgctca ggccttcgcg ctgcactcgc tgctcgacgc
     1261 cgactacgtg cagttccagc tgaccgagcg cggccagacg ctcgagacgc ttggcgacgc
     1321 gtgggaaagc gtggcgcgca aactctgcgg catatgacgc cgacgtgggt cctgcatgtt
     1381 gacctcgacc agtttcaggc cgcagtcgaa gttcgccgac ggcccgaact tgccggcctg
     1441 ccaatcattg tcggcggcaa cggtgatccg aatgaaccac gcaaggtggt cacatgtgcg
     1501 tcctacccgg cgcgcaagtt cggggtgcac gccggcatgc cgctgcgcac cgcggcccgc
     1561 aagtgtcccg atgcgacgtt cctgccgctg gataccgacg cctatgacgc cgcgtccgaa
     1621 gaggtgatgg acctgctgcg cgacctcggc catcccgtcg aagtgtgggg ctgggacgag
     1681 gcctacgtcg gcgcgaacgt cgacgctccc gtacggctgg ccgagcagat ccgtgaggtc
     1741 atcggccaga cggagttgtc gtgttcggtc ggcatcagcg acaacaaaca gcgcgccaag
     1801 gtcgccaccg ggttcggcaa gccggcaggc atctatcagc tcacggacga gaactggatg
     1861 gcggtaatgg gcgatcgcga ggtcgatgcc ttgtggggtg ttggaccgaa gacggccaaa
     1921 aagcttgccg gcatgggcat taccaccgtc gccgaccttg ccgccaccga cgccacgctg
     1981 ctcacgtcga cgttcgggcc gacgactgga ctgtggattc tgctgctggc caagggcggt
     2041 ggcgacacca acgtcagcgc cgaaccgtgg gtgccccgct cacgcagcca cgtcatcacg
     2101 ttcgcgaagg acctaaccga tcgcacggaa atcgattctg cggtcgtcga gctcgcgcag
     2161 cagacgctca aagagattgt cgcccaacgt cgcatcgtga ctcgagtcgc ggtcaccgtg
     2221 cgcaccaaca ccttctatac ccggaccaag attcgtaagc tgcccgcgcc cagtaccgac
     2281 gccgacctca tcaacaaaac cgcgctggac cttctcggcc tcttcgaact cgcccgaccg
     2341 atccggctgc tcggcgtacg gctggagctc aacgctccgg aaggagggta ctgatcatcc
     2401 atgctgcaca ccgtcgcaat ccgcggttac cgctcactac gcgaaatcgt gctgccgctc
     2461 gcccggctca ccgtcgtcac cggcgccaat ggcacgggca agtcctcgct gtatcgagcc
     2521 ttgcgcctgc tggccgactg cggacgcggc gaggtgatcg gctcgctggc acgcgagggc
     2581 gggctggact cggtgctgtg ggcaggtccc gagcatctca gcggcgcacg ccgaacgggc
     2641 aaggtcgagg gcacgacccg aaccgggccg gtttcacttg aactcggatt cgcctctgac
     2701 gatttcggct atctggtcga tctcgggctg ccgcagatgg ccgggcacaa ttcattgttc
     2761 gcactcgacc ccgaaatcaa gcgcgaggcg gtcttcgcgg gccgggtgat gcggaccagt
     2821 tcgacgttgg tgcgccgcac ccgcgactac gccgaagcgt ccgcggaatc cggccgaggc
     2881 ttcgacgaga tgacgcgatc gctgccgctc taccgcagtg tgctcgccga atacgctcac
     2941 cccggcgcgc atcccgaact ggctgccgtc cgccatcggc tgcgcagctg gcggttctac
     3001 gacggctttc gggtcgatat ggccgcgccg tcgcgactcc cccacgtcgg cacccgcaca
     3061 cctgtgctct ccgacgacgg cggcgatctg gcggccgccg tgcagacgat catcgaagcg
     3121 ggattcgacg acctccagcg tgcggtcgcc gacgcgttcg acggagcgac cttatcggtg
     3181 gccgtccatg acgggctgtt cgatctgcag ctgcaacaac gcggcatgtt gcgtccccta
     3241 cgcgccgccg aattgtccga tggcacactg cgattcctgc tgtgggccgc cgcgctgctg
     3301 agtccgcagc cgccatcgct gatggtgctc aacgagccgg aaacctcact gcacccagac
     3361 ctggtgcggc cattagcgtc gctgattcag gctgccgccg gtcagacgca ggtcgtcgtt
     3421 gtcacgcact cgcgagcgat gctggagttc ctggacactg tgcccatcac cgacgccgaa
     3481 cagggcggcg cggtggaagt cgagttgtac aaggaacttg gtgagacgcg cgtcaagggg
     3541 ttgggcctgc tgaccacgcc gccgtgggac tggggtaagc gctaaccgcg ccgcgcaggc
     3601 cgcattacgc gggtgaagaa gacgttgacc cgtttcatcg caatgctgta cggccaccac
     3661 gccgtccgct tgaacaggta cagcgcgcgg atgtcgggtg aggtgtagat caggaatcgg
     3721 ttctttcgca cgccgccgag aatccggtcg gcgacgtgtt cgggcgatac cgcgtgcccg
     3781 ctgaagcggt tggtccactt ctgcacgcgc gggtcgtcgc ggtccacgcc ggcgatctga
     3841 accgattgca ccagaggcgt tttcacagcg ccgggcgcga cgaccgagac cccaatgcga
     3901 tgccggtcca gatcgaaccg caacacttcg gacaacccgc gcaggccgta tttgctcgcg
     3961 ctgtaggcgg cgtgccacgg cagcgcgacc agtcccgcgg ccgacgacac gttgaccaga
     4021 tgaccgccgc ggcccgcggc gaccatcggc ggcacgaacg tctcgatgac gtgaatcggg
     4081 cccatcaggt tgatgtcgat catcgatcgc cagtgctgat gcgtgagttg ctcgacggtg
     4141 ccccacgccg acacaccggc gatgttcatc acgacatcca ttgccgggtg gcgggtgtgg
     4201 atgtcgaccg caaatgcggc gacctcgtcg tagttggaga tgtcgagtgc ccggtactcg
     4261 gccaccgtcg cgcccagcgc ctcggcgtcg aggactgtct tctccagtcc atccgcgtcg
     4321 cgatcagtca gatagagctc agcgccgtcg gcggctaatt tcagcgcggt cgcgcggccg
     4381 atgccgctgg cggccccggt gatgaaacaa cgtttgcccg aaaagccgct cccctgcgcc
     4441 atggcggtga cgataccgtc gccgctactc gtcaccccac agcgcgttca gccacagccg
     4501 ctccacgatg tcgaccgcgc gtgccggatc ctcgccgcgg cccacgaacg cgctgtcctg
     4561 cgtcagcgtg aacgacgtca ccgcagccag cgtccgcacc aacgcaggta gatcgtcgga
     4621 gatcgggcgc gcatcggagt cctgctcgag aagggtgatg agcttgtcga tgatgccgtc
     4681 gtagaagtcg tccatcatct cgcggatctg cgcgtcggta tttcgagcca ccgcgcaggc
     4741 cttcaacacg ggatcgtcgt tggcgtacac cgcggccgcg ctgccgacca tccgcttggc
     4801 gaaggcggcg ggtgtctcgc ccggctcgcg cggcgcaaaa tggtccgtca ggttgtcgag
     4861 caggtcaccc gcgtcggcaa ggatcaccgc gagcaccgcg tacttggagt cgaagtaaaa
     4921 gtagaaaccg gaccgtgcga cgccggcacg ctcactgatc gtgctgacgg acaggtcggc
     4981 gaacgaccgc tcctgcagca actcgcgaac cgcgttcacg atggcctcgc gttgccggtc
     5041 cccgcggctg cggcggatct gctgtggtgc gggttgcgcg gtcatggcct tagaccttcg
     5101 caccgcgacg cgccagaaca aaacttgaca tgcgtcaagt ctgccatcca ggatgaagat
     5161 aagtggtgac aacggccaca gtcttttcct tcaggagcgt gtcaatgccg acgatcagca
     5221 ccaaggacta cctgctcgac caggccaggc ggcggttgaa gccgactgcg gtcacgatcc
     5281 ccgggatggg cctggccgaa aagcggctgc gcaagcacga gttcaaacag ttccctctcg
     5341 ctgagccgcc gacgggcagt ggcctcaagc cggcaatggg cgactcgggg ttgccgatca
     5401 tcggccacat gatcgagttc ttccgcatgg ggccggactt cgccgtggag atataccgca
     5461 agtacggccc ggtccactac caatactcac ccgcactgtc gtcggtgatg gcgttgggac
     5521 ccgaagccac gcaggccgtc ttctccaacc gcaacaagga gttctcccag cgggcctggg
     5581 atccggtcat cggtccgttc ttcgatcgtg gcctcatgct gctcgacttc gacgagcaca
     5641 tgtaccaccg ccggatcatg caggaggcgt tcacccgcac gcggctgtcg ggctacgtcg
     5701 agcacatcga ctcggtggcc agccatgtgg tggccaatga ctgggtcgcc aacgacccac
     5761 ggttcctgtt ccatccggcc gccaaggagc tcacgctcga catcgcctct gtggtgttca
     5821 tgggccatga gcccggcacc gaccgcgaac tggttaccac cgtcaaccag gccttcacca
     5881 cgaccacccg agccggcaac gcgattgtgc gcaaacccgt acgaccgctg acctggtggc
     5941 gcggcatcca ggcccgcaag actctcgagg actatttcgc cgcccggctc accgagacgc
     6001 gcggttccga gggcaccgac atgctcagtg tgctgcgcca agccgaggac gaggacgggc
     6061 agcggttcac cgacgccgac atcgtttcgc acatgatctt cctgatgatg gccgcccacg
     6121 acacgtcgac gtcgacaatg acgacgatgg cctaccacct tgccgccaat ccggattggc
     6181 aggagcgctg ccgcgacgag tcggcacgca tcggcgacgg gccgctggac atcgaggcgc
     6241 tggagaagct ggaaacgtac gacctggtga tcaacgaggc cttgcgcatg gtcacgccac
     6301 tgccgatgaa cttccgccgg gcggtgcggg acatcgatct gcttggctac ttcatcccgg
     6361 cgggcaccga cgtcgtgacg tggccgtcgc tcaaccaccg gctgcccgag ttgtggacgg
     6421 acccggagaa attcgacccg gaccgcttcg ccgaaccgcg ctcggagcac aagaagcacc
     6481 ggtacgcgtt cgcgccgttc ggcggcggag cgcacaaatg catcggcatg gtcttcggtc
     6541 agctcgagat caagaccgtc atgcaccggc tgctgcgtaa gtaccgactc gagttggtcc
     6601 gccccgacta caagccgcgc tacgactacg gcggcatgcc ggtccccgtc gacggcatgc
     6661 cgatcgtcct gcgcccgctg cggtagcgct caggcctcgg tcagaagccg gttcgcgcag
     6721 gcgataaccg cacgcaacgc cgactgcgtc gggtcgtcgg accagcccat cgcccactcc
     6781 gaccgggcac cgtcggaccc gcggacgaac gtcgcggtgt gctcgcctgc cggaacctgg
     6841 tgaaaggccg tcatctcgac cgcgatgccg cggtcgtaca gcatctcggt cagcgccgcg
     6901 accggtccgc atgcggctgc cgacgatgta ctgatgcgat cgccgaacgc cagtgtcgcc
     6961 tggtagttgc gggcctgcgg acccaggcgg gtggacggcc ggtgggtgtc ggtgcattgc
     7021 cagttcccga gccgcagcgg tcctgggctc gatgcatatt cgtcgtgaaa cgcctcgaaa
     7081 gacatcgcgt cggcctcctg gcgcaggccg cggggcatag ggccgtcgac gtgggccgca
     7141 aatgtcagcg gcgctgcgct ggtagcggat gtgtttctca tgtgccggtc ttctcgggtc
     7201 agaggaagtg accgacgtaa tagcaacgac ccacagcgag gggtcggtca ggatcagacc
     7261 ccgctgcggg ttgctactac gattcgcctc ggcacgatcg cgatgctagg cgctgaaccg
     7321 caccctgtgc aaacgaaatt tggtttcatg tttcggatag caccctgagc atttgttcgc
     7381 caatcggctg tacccgtagc gcgtccggtg gcattctctt agcaccttta ctggaaaatg
     7441 gtgggggtgg attgaacacg cagactccgc ggactcggga taccgcgata ccagtgggcg
     7501 accgcgtcgg ttcgttccga tatttgcgcg ccgaggatcg gtgggagtgg tcgggcgccg
     7561 tcgcgcagat gcatggctac gcgccagggc aggtgcaccc cacgactgcg ttggtgatgt
     7621 cgcacaaaca tcccgacgac gccgcgaacg tggcgcg
//
LOCUS       DASRJA010000203         2518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3662907,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000203 DASRJA010000000
VERSION     DASRJA010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3662907"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2052
                     /locus_tag="VFC01_09135"
     CDS             <1..2052
                     /locus_tag="VFC01_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZN79834.1"
                     /translation="ETKHPAFAQPFHTLDADALRAAKLHAAAIASRGSRGPSIAPHAP
                     LAGLFNNLNSPGLNQLTATPPDSTGAIGPNNYVEMVNQEIGVYDRNLDLISSTDNGTF
                     TGAGAGSSVTDPQIQYDPQSGHWLYVALGVAQGANLLIFGWSKTSDPSDLTNGWCRFG
                     TGRGNLLDDYPKLGHDNNFISIGTNVYDDTNAPNYTFLTANIFVIRKPQAGDASCSVD
                     TVLYVSDATHPLHNADGSTAFTPVPANTVDSSSLGYIVAAHSPVDGVGSSAPKVMVWH
                     WTSVGGTPSLVADGDVTVPTFDIPAPVPQPGTSYKLDSLDARLTQAVAVNDQGAGGAK
                     GIWTQHTVAGPGGRSVVRWYEILGGTPPTLRQHGDVGSPTDFVFNGAISPSIGGSSAA
                     VFYNRGGASTLPVIGAQTRTASTPLGSLDPGELVIGQSSAADLDFSCGVPSATSPCRW
                     GDYAGATPDPLNDGVVWGSSQVTGPCFIFCGFFAQWQTQNFAVVASTSPPPPPVLTSI
                     TVTPATASVQNGGSQQFSATGYDQNNVPMNPQPTFTWSVSGGGSINSAGLFTATTVGS
                     NFTVTAASGGVNGTAKVTVTDFSLSVSPTSRSIKRNQTATYGVTITRLNGFAGSVLFT
                     VSGLPSNATASVTPNPTTGTSVTVKISTSRSSPIGPFTLQIRGSSGGLAHAINVSLIL
                     R"
BASE COUNT          441 a          896 c          773 g          408 t
ORIGIN      
        1 gaaaccaagc accctgcctt cgcgcaacct tttcacacgc tggacgcgga cgcccttcgg
       61 gcggccaagc tccatgctgc ggcgatcgct tcccgcggct cccgcggtcc atccatcgcc
      121 ccgcacgccc cactcgccgg actgttcaac aacctcaaca gcccgggcct caaccaactg
      181 acggcgacgc cgcccgacag cacgggtgcg atcggtccga acaactacgt cgagatggtc
      241 aaccaggaga tcggcgtcta cgatcgcaac ctcgatctca tcagcagcac ggacaacggc
      301 accttcacgg gtgccggtgc cggctcgtcg gtcaccgatc cccagatcca gtacgacccg
      361 cagagcggcc actggctgta cgtggcactc ggggtcgcgc agggtgccaa cctgctcatc
      421 ttcggctggt ccaagacctc cgacccgagc gatctgacca acggctggtg tcggttcggt
      481 accggccggg gcaacctgct cgacgactat cccaagctcg gacacgacaa caacttcatc
      541 agcatcggga cgaacgtcta cgacgacacc aacgccccga attacacctt cctcaccgcc
      601 aacatcttcg tgatcaggaa gccgcaggcc ggtgacgcaa gctgctctgt cgatacggtc
      661 ctgtacgtct ccgacgccac gcatcccctc cacaacgcgg atggttcaac cgcgttcacg
      721 cctgtcccag ccaataccgt cgattcgtcg tcgctcggct acatcgtcgc tgcacattca
      781 ccagttgacg gtgtcggcag ctccgcaccg aaagtcatgg tctggcactg gacgtcggtc
      841 ggcggcacgc cgtcgctcgt ggccgacggc gacgtgaccg tcccgacctt cgacatcccg
      901 gcaccggttc cgcagccggg cacctcatac aagctcgact cgctggatgc gcggctcacg
      961 caggcggtcg cggtcaatga tcagggtgcc ggcggtgcga aggggatctg gacacagcac
     1021 acggtcgctg gtcccggtgg tcgctccgtg gttcgctggt acgagatcct cggcggcaca
     1081 ccgccgacgc tgcgacagca tggcgacgtt ggtagcccga ccgacttcgt cttcaacggc
     1141 gcgatctctc cttcgatcgg cggcagcagc gccgccgtct tctacaacag aggcggggca
     1201 tcgactctgc ctgtgatcgg ggcgcagaca cgaaccgcgt cgacacccct cggcagcctc
     1261 gatccgggcg agctcgtcat cggtcagtcc agcgccgccg acctcgactt cagctgtggc
     1321 gtcccctctg cgacgtcccc gtgccgttgg ggcgactacg cgggggcgac cccggatcca
     1381 ctgaacgacg gcgttgtctg gggaagcagc caagtcaccg ggccgtgctt catcttctgc
     1441 gggttcttcg cgcaatggca aacgcagaat tttgcggtgg tcgcctccac atcgccgcca
     1501 ccgccgcccg tcctgacctc gatcacggtg acacccgcga ccgcctcggt ccagaacggc
     1561 gggtcccagc agttcagcgc caccggctac gaccagaaca acgtcccgat gaacccgcag
     1621 ccgacgttca cctggtcggt cagcggtggc ggctcgatca actcggcggg cctgttcacg
     1681 gccacgaccg tcgggtcgaa cttcacggtg accgccgcga gcggtggcgt gaacggcacg
     1741 gccaaggtca cggtcactga cttctcgctc tcggtgagcc cgacttcgcg gtccatcaag
     1801 cgcaaccaga ccgccaccta cggagtgacg atcacgcgcc tgaatgggtt cgcgggctcc
     1861 gtgctcttca cggtcagcgg actgccgagc aacgcgaccg cgtccgtaac tccgaatccc
     1921 acgactggca ccagcgtcac ggtgaagatc tcgacctccc gaagcagccc catcggaccg
     1981 ttcacgctgc agatcagggg gtcgagcgga ggcttggcgc acgcgatcaa cgtctcgctc
     2041 atcctcagat aggctcgccc ctggtcggtc gatgctggcc agggagccga ttgacaacga
     2101 gccctgaccg agcgttgagg agacggcgac gctcggtgag ctggcgcggg gtcaccgact
     2161 gccgcgcttg aggctcaacc accacgtcgc tagcaagact gccgtggcga ccgcaatcgc
     2221 gaggagccaa tcaccgcgtg gccgccgcca gctgctggcc ggtcaactcg gcgagtgctg
     2281 aacggactgc gtcgtagtcg acgactcggg ggaggacgac gccgaggttg acgccgaaga
     2341 tcgcgacgag ggtgatgatc ctcgtggcga tcgaccgccg gttgggcctc gcggatggcg
     2401 aaagcgaacc ggcggtcggt ttatcgagtg gggcgcctac atccgctcgt ggcggtcggc
     2461 gcccagtcag cggctagccg tcgccagcgc gtccgttgcc tcccggcgtg ccaggatc
//
LOCUS       DASRJA010000204         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2113168,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000204 DASRJA010000000
VERSION     DASRJA010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113168"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VFC01_09140"
     CDS             complement(<1..1194)
                     /locus_tag="VFC01_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HZN79835.1"
                     /translation="MSDIEIAQWRDRKRYLWLMGLIAPTALFVMLPLVWAFHQWGWHV
                     AAQVPFWIGPILLYILLPALDLRFGPDGQNPPDEVMERLENDKYYRYCTYIYIPFQYA
                     SVILGAYLFTASDLGWLGFDGGLSWPAKIGLALSVGVLGGVGINTAHEMGHKKDELER
                     WLSKVTLAQTFYGHFYIEHNRGHHVRVATPEDPASARFGETFWEFLPRSVFGSLRSSW
                     ELEAQRLRRAGRSPWHWSNDVLNAWAMSVVLFGILIAVFGPALIPFVVIQAVFGFTLL
                     ETVNYLEHYGLLRQKTESGRYERCAPVHSWNSDHIVTNLFLYHLQRHSDHHANPTRRY
                     QTLRSMEGAPNLPSGYASMIALTYFPPLWRRVMDHRVLEHYGGDITRVNVHPRVHDKV
                     ISCYGV"
     gene            complement(1344..>2661)
                     /locus_tag="VFC01_09145"
     CDS             complement(1344..>2661)
                     /locus_tag="VFC01_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN79836.1"
                     /translation="AGNITGPAISISFVIAATACGLAALCYAEFASTVPVAGSAYTFS
                     YATFGEFIAWIIGWDLILEFAVAAAVVAKGWSSYLGTVFGFGGGTADFGGFQVDWGAL
                     LIIAFVTVILAWGTKLSAEVSLAITVVKVAVVLLVVVVGAFYIKTANFSPFIPPAEAG
                     HGGTGSEQSLFSLMTGAEGSHYGWYGVLAGASIVFFAFIGFDIVATTAEETKNPQRDV
                     SRGILASLAIVTVLYVAVAFVLSGMVSYTELRDAGEKANLATAFAVNGVDWAAKVISI
                     GALAGLTTVVIVLVLGQTRVLFAMCRDGLLPRPLARTGPHGTPVRITLIVGVLVAITA
                     SVFPIGKLEEMVNIGTLFAFVLVSAGVIVLRRTRPDLERGFRAPGVPLLPIASIVACL
                     WLMLNLTGLTWIRFLVWMAIGVLVYFLYGRSHSVLAQRESGVISAP"
BASE COUNT          547 a          889 c          810 g          415 t
ORIGIN      
        1 cactccgtag caggaaatca ccttgtcgtg cacccgcggg tgcacgttga cccgggtgat
       61 gtcgccgccg taatgctcga gcacccggtg atccatcacg cggcgccaca gcggcgggaa
      121 gtacgtcagc gcgatcatcg atgcatagcc gctaggcagg ttcggcgcgc cctccatgct
      181 gcgcagcgtc tgataccggc gtgtcgggtt ggcgtggtga tcgctgtgcc gctgcaggtg
      241 atacaggaag aggttggtca cgatgtgatc ggaattccag ctgtgcaccg gcgcgcaccg
      301 ctcgtagcga ccgctttcgg tcttctgccg cagcagcccg taatgctcga ggtaattcac
      361 cgtttccagc agcgtgaatc cgaacacggc ctggatcacg acgaatggaa tcagcgccgg
      421 accgaagacg gcgatcagga tgccgaagag caccaccgac atcgcccatg cattgagcac
      481 gtcgttggac cagtgccacg gactcctgcc ggcccgacgc agccgctgcg cctccagctc
      541 ccaggacgag cgcaggctgc caaacacgct tcgcggcagg aactcccaga aggtctcgcc
      601 gaagcgggcc gacgcgggat cctcaggggt ggccacccgc acatggtggc cgcggttgtg
      661 ctcgatgtag aagtggccgt aaaacgtctg cgccaatgtg actttcgata gccaccgctc
      721 gagttcgtcc ttcttgtgac ccatttcgtg cgcggtgttg atgcccaccc ctccgagcac
      781 acccaccgac agcgccaaac cgatcttggc cggccaactc aatccgccat caaatcccag
      841 ccagcccaga tcggacgcgg tgaacaggta ggcacccaag atcacgctcg cgtactggaa
      901 cgggatgtag atgtaggtgc agtagcggta gtacttgtcg ttctccagcc gctccatcac
      961 ctcgtcgggc gggttctgac cgtcagggcc gaagcgcagg tccagggcgg gcagcaggat
     1021 gtagagcagg atcgggccga tccagaacgg cacctgcgcc gcgacgtgcc agccccattg
     1081 atggaacgcc cacaccagcg gcagcatcac gaacagcgcc gtcggtgcga tcagccccat
     1141 cagccacagg tagcgctttc tgtcgcgcca ttgggcgatc tcgatgtctg acatttgggc
     1201 ggtcactaca tgcctcctcg tgtgagtgcc atcacgtcgt tgagttgact ataggacccg
     1261 ttttgtcgtc tgtctagaca caaaggcatg ttttgtaaaa caggtgcggc gcgcttcagc
     1321 gatttcggcg cgtttagcgt ggattacggc gccgaaatca ctccggattc gcgctgggcc
     1381 agcaccgagt ggctgcggcc atagaggaag tagacgagca cgccgatagc catccagacg
     1441 aggaaccgaa tccacgtcag gccggtcagg ttcagcatca gccacaagca ggccacgatc
     1501 gacgcgatcg ggagcagcgg cacccccggc gcccggaacc cacgctcaag gtccggacgg
     1561 gtgcggcgca gcacgatcac ccccgccgac accagcacga acgcgaacaa cgtgccgatg
     1621 ttgaccatct cctcgagctt gccgatcggg aacaccgacg ccgtgatggc caccagcacc
     1681 ccgacgataa gagtgattcg caccggcgtg ccgtgcggac cggttctagc cagcggtcga
     1741 ggcagcaagc cgtcgcggca catcgcgaac aacacccgcg tctggccgag caccagcacg
     1801 atgacgacgg tggtcagccc cgccaaggcg ccgatcgaaa tgaccttcgc ggcccagtcc
     1861 acgccgttga cggcgaacgc ggtcgcgaga ttcgccttct ctcccgcatc gcgaagctcc
     1921 gtgtagctga ccatgcccga caacacgaac gccaccgcca catagaggac cgtgacgatc
     1981 gccagcgagg cgagaatccc ccgcgacacg tcgcgctgcg gattcttggt ctcctcggcc
     2041 gtcgtcgcga cgatgtcgaa gccgatgaac gcgaagaaca cgatcgaggc gcccgccagc
     2101 acgccgtacc agccgtagtg gctgccttca gcaccggtca tcagcgagaa caacgactgt
     2161 tccgaacccg tgccgccgtg gccggcctcg gcgggcggaa tgaacggcga gaagttcgcc
     2221 gtcttgatgt agaacgcgcc gacgacgacc accagcagca ccaccgccac cttgaccacc
     2281 gtgatggcca ggctgacttc tgccgacaac ttggtgcccc aggcgaggat gaccgtgacg
     2341 aacgcgatga tcagcagcgc gccccagtca acttggaaac caccgaaatc ggctgtgccg
     2401 ccgccgaatc cgaacaccgt gcccagatag ctcgaccagc ctttcgccac cacggctgcg
     2461 gcgacggcga actccaggat caaatcccag ccgatgatcc acgcgatgaa ctcgccgaag
     2521 gtcgcatagg agaacgtgta ggcgctgccg gccaccggca ccgtcgaggc gaactccgca
     2581 taacaaagcg cggcgaggcc gcacgcggtc gcggcgatga cgaacgagat cgagatcgcc
     2641 gggccggtga tgttgcccgc a
//
LOCUS       DASRJA010000205         6515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_997318,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000205 DASRJA010000000
VERSION     DASRJA010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997318"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFC01_09150"
     CDS             complement(<1..339)
                     /locus_tag="VFC01_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN79837.1"
                     /translation="MTHGPVGDSSSVVEYEETEHAGRVVRIASVAALGGLLFGYDSAV
                     INGAVSAIEGEFGVGGFKLGFAVASALLGAALGAVTAGRLADKIGRLAVMKIAALLFL
                     ISAIGAGLSYN"
     gene            complement(396..1079)
                     /locus_tag="VFC01_09155"
     CDS             complement(396..1079)
                     /locus_tag="VFC01_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZN79838.1"
                     /translation="MQAVLFDMDGTLVDSEKLWDVSLAALYEELGGRLTPEVRASMVG
                     GSAEDTMRTVYTDLGLELDPVAMTDSIRWLHDYTAELFDNGLPWCDGAQDLLEALAAE
                     GTPMALVTNTQRPLTDRALNSIGRRYFSATVCGDEVPRGKPAPDAYLRAAALLDLPPS
                     ACLAIEDSVTGTAAAEGAGCPVLVVPNDVAVPRGPRRRHISSLTGVAVGDLSRVFSEL
                     TSQFGERTA"
     gene            1120..1923
                     /locus_tag="VFC01_09160"
     CDS             1120..1923
                     /locus_tag="VFC01_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZN79839.1"
                     /translation="MTILVTGATGNIGRRIVDHLIDLGANDIRALTKNPSKAKLPDGV
                     TAVTGYLGDPESLPAAFDGVERMYLAPLPTTLDVTLELIRKANVEYVVALSGGGDWQE
                     HADKVTASGLVNTQLGPGEFTDNFTIWADQIKASQTVRDPYPSVVEAPISMDDIARVA
                     AALLIKPDESHHGKMYPITGPEALTRADIAKQIGVGIGVDITFEQCSRADGEAALRPA
                     MGEYAHWYLDQIETGIDTPQQANQLVADLTGAPAQSVAQWAAQNAEMFR"
     gene            complement(2009..5584)
                     /gene="metH"
                     /locus_tag="VFC01_09165"
     CDS             complement(2009..5584)
                     /gene="metH"
                     /locus_tag="VFC01_09165"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HZN79840.1"
                     /translation="MTVVNDRAYDTDLLDTLSKRVMVGDGAMGTQLQAADLTLDDFNN
                     LEGCNEILNDTRPDVIETIHRAYFEAGADAVETNTFGCNLSNLGDYDIADRIRDLSQR
                     GTAIARRVADEMSTPDRRRYVLGSMGPGTKLPTLGHTEYAVIRDAYAEAALGMLEGGA
                     DAILVETCQDLLQLKAAVLGSRRAMAQLGRHIPVIAHVTVETTGTMLLGSEIGAALTA
                     VEPLGVDMIGLNCATGPAEMSEHLRYLSQHARTPVSVMPNAGLPVLGANGAEYPLTPD
                     ELAEALSGFVSEYGLSLVGGCCGTTPEHIRKVADAIPNVQRAERHATYEPAVASLYSS
                     TPFAQDASVLMIGERTNANGSKAFREAMIAEDWQKCLDIAKEQTRDGAHLLDLCVDYV
                     GRDGVADMTALASRLATASTLPIMLDSTETPVLKAGLEHLGGRCAINSVNYEDGDGPE
                     SRFHKTMELVAEHGAAVVALTIDEEGQARTAEKKVEIAERLIADITGNWGVDESSILI
                     DTLTFTICTGQEESRKDGIETIEAIRLLKQKHPDVQTTLGLSNISFGLNPAARQVLNS
                     VFLHECQEAGLDSAIVHASKILPMNRIDEKQREVALDLVYDRRREGYDPLQELMGMFE
                     GVSAASSKESRAAELAKMPLLERLAQRIVDGERNGLEADLDEAMTQKPPLSIINDDLL
                     AGMKTVGELFGSGQMQLPFVLQSAEVMKTAVAYLEPHMEKAEDDAGKGRIVLATVKGV
                     VHDIGKNLVDIILTNNGYEVVNIGIKQPIANILEVAQDKSADVVGMSGLLVKSTVVMK
                     ENLQEMNTRGVADRFPVLLGGAALTRSYVENDLADVYEGEVHYARDAFEGLKLMDVIM
                     SAKRGEVPDTDSPEAVAAREKEAERKARHERSKRIAAERKAKETPVVVPARSNVAADV
                     EVPVPPFWGTRVVKGVAVADYTSTLDERALFLGQWGLRGVRGGEGPSYEELVETEGRP
                     RLRYWLDRLSTEGILAHAAVVYGYFPAVSQGDDVIVLTEPKPDAAERFRFTFPRQQRD
                     RFLCIADFIRSRELANERGEVDVLPFQLVTMGQPIADFANELFKSDSYRDYLEVHGIG
                     VQLTEALAEYWHKRIREELRFPDHVMAAEDPDSVEDYFKLGYRGARFSFGYGACPNLE
                     DRAKMMELLEPDRIGVTLSEELQLHPEQSTDAFVLHHPEAKYFNT"
     gene            5667..>6515
                     /locus_tag="VFC01_09170"
     CDS             5667..>6515
                     /locus_tag="VFC01_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HZN79841.1"
                     /translation="MTPSDASGHRPADLPKLNDTIVVAAFEGWNDAGDAASDALEHLD
                     AIWEAEPIVEIDDEAYYDYQVNRPVIRQVDGVTRELVWPSMRISHCRPPGSDRDIVLM
                     HGVEPNMRWRTFCAELLAIADKLNVQTVVILGALLADTPHTRPVPVSGAAYSPDSAKT
                     FGLEETRYEGPTGIAGVFQDACVQAGIPAVTFWAAVPHYVSQPPNPKATVALLRRVED
                     VLDIEVPLADLPSQAEEWEQAVSEMTSEDDEIAEYVQSLEERGDAEVDMTEALGKIDG
                     DALAAEF"
BASE COUNT         1151 a         2165 c         2072 g         1127 t
ORIGIN      
        1 gttgtaggaa aggccggcac cgatcgcgct gatcaagaac agcagcgccg cgatcttcat
       61 cacagccaac cggccgatct tgtcggcgag cctgcctgcg gtgacggcgc cgagggctgc
      121 cccgagcaac gccgaggcca ccgcgaagcc gagcttgaaa ccgccgacgc cgaactcgcc
      181 ctcgatcgcc gagaccgcgc cattgatcac cgcgctgtcg tagccgaaca gcagcccgcc
      241 gagtgcggcc accgacgcga tccggacgac ccgaccggcg tgctcggttt cttcgtactc
      301 cactacagaa gaggaatccc caaccggtcc gtgagtcatg tgacgccctt ccgcagcatt
      361 gcgtgatgag aatcacatta accgcgcaat ggggatcagg cggtgcgttc tccgaactga
      421 gacgtgagtt ccgagaagac ccgggataag tcgccaacag cgacgccggt gagcgacgag
      481 atgtgtcgcc ggcgcgggcc gcgcggcacc gccacatcgt taggtaccac cagcacaggg
      541 catcctgcgc cttccgctgc ggcggttccg gtgactgaat cctcgatagc caaacacgcc
      601 gacggcggca gatccagcaa cgcggccgcc cgcagataag catcgggtgc gggtttgccc
      661 cgcggcacct cgtcgccgca cacggtcgcc gaaaaatacc ggcgaccaat gctgttcaat
      721 gcccgatcag tgagcgggcg ctgagtgttg gttaccagcg ccatcggagt cccttcggcg
      781 gcaagcgctt ccaacaaatc ctgcgcgccg tcgcaccacg gcaacccgtt gtcgaacaac
      841 tctgctgtgt agtcgtgcag ccagcggatg gagtcggtca tcgcgaccgg atccagctcc
      901 aggccgaggt cggtgtaaac ggtgcgcatg gtgtcttccg cggagccgcc caccatcgac
      961 gcgcgcactt cgggagtcaa cctgccacca agctcttcgt agagcgccgc gagcgagaca
     1021 tcccagagct tttcggagtc gacgagcgtg ccatccatat cgaacagcac cgcttgcatg
     1081 tcagcgattc tgtcatggtt cgcacgtacg gtgaaaatca tgacaattct cgtgaccggc
     1141 gccacaggaa acatcggccg tcgcatcgtc gatcacctga tcgacctggg cgccaacgac
     1201 atccgcgcac tgaccaagaa cccgtcgaag gcgaagctgc ccgacggggt gacggccgtc
     1261 accggatacc tcggcgatcc cgaatcgctg cccgccgcgt tcgatggcgt cgagcgcatg
     1321 tatctggccc ccctgcccac cacacttgac gtcacacttg agctcatacg caaggccaat
     1381 gtcgagtatg tggtcgcgtt gtccggcggc ggtgactggc aggagcacgc agacaaggtc
     1441 acagcgtcag ggctggtaaa tacccaactc gggccgggcg agttcaccga caacttcacg
     1501 atctgggccg accagatcaa ggccagccag acggtccgcg atccgtatcc ctcggttgtc
     1561 gaggcgccga tctcgatgga tgacatcgcc cgagtcgccg ccgcgctgct gatcaagcct
     1621 gacgaatcgc accacggaaa gatgtatccg atcaccggcc cggaagcgct gacccgtgcg
     1681 gatatcgcca agcagatcgg cgtcgggatc ggcgtcgaca tcacgttcga gcaatgcagc
     1741 cgcgccgacg gcgaggccgc gttgcggccg gccatgggcg agtacgcgca ctggtacctc
     1801 gatcagatcg agaccgggat cgacacacct caacaggcca atcagctggt cgccgacctg
     1861 accggcgcac cggctcagtc cgtggcccaa tgggccgcgc agaacgccga gatgttccgc
     1921 tgaacagctc gaagaagtcg gcccagtggg aacttaaggc acgcccgagg tgcgatcggc
     1981 gtgcaataag tggccactcg acgacgacct aggtgttgaa gtacttcgcc tcggggtggt
     2041 gcagcacgaa cgcgtccgtg gactgctccg ggtgaagctg caactcctcg gacagcgtca
     2101 caccgatgcg atcgggttcc agtagctcca tcatctttgc gcggtcctcg aggttcgggc
     2161 aggcgccgta gccgaacgag aaccgggcac cgcggtagcc gagcttgaag tagtcctcga
     2221 ccgagtcggg gtcctcggcg gccatcacgt gatcggggaa ccgcagctct tcgcggatgc
     2281 gcttgtgcca gtactcggcc agtgcctcgg tcagctgcac accgataccg tgcacctcga
     2341 gataatcgcg gtaggagtcc gacttgaaca actcgttcgc gaagtcagcg atgggctgtc
     2401 ccatggtcac caactggaac ggcaacacgt caacctcgcc gcgctcgttg gccagctccc
     2461 gcgaccggat gaagtcggcg atgcacagga accggtcacg ctgctgacgc ggaaaggtga
     2521 accggaaccg ttcggccgca tcgggtttgg gctcggtgag cacgatgacg tcgtcacctt
     2581 gagacaccgc cgggaagtag ccgtacacca cggccgcgtg cgcgaggatg ccctccgtcg
     2641 acagccggtc cagccagtac cgcaggcgcg ggcggccttc ggtttcgacg agctcttcgt
     2701 atgacgggcc ctcaccgccg cgcacgccgc gcaatcccca ctggccgagg aacagcgcac
     2761 gctcgtcgag cgtgctggtg tagtcagcca ccgcgacacc tttgacgacg cgggtgcccc
     2821 agaacggcgg gaccggcacc tcaacatcgg cagcgacgtt cgagcgcgcc ggcaccacga
     2881 ccggcgtctc cttggctttc cgttcggccg caatgcgttt cgaccgctcg tggcgagcct
     2941 tgcgctcggc ctccttctcg cgtgccgcga ccgcctcggg gctgtcggtg tcggggacct
     3001 cgccacgctt ggcgctcatg atgacgtcca tcagcttgag gccctcgaac gcgtcacggg
     3061 cgtaatgcac ttcgccttcg tagacgtcgg cgagatcgtt ctcaacgtag ctgcgggtga
     3121 gcgccgcgcc gccgagcagt accgggaacc ggtcggccac cccgcgagtg ttcatctctt
     3181 ggaggttttc cttcatcact acggtggact tcaccagcag ccccgacatg ccaacgacgt
     3241 cggcgctctt gtcctgggcc acctcgagga tgttggcgat cggctgcttg atgccgatgt
     3301 tgacgacctc gtagccgttg ttggtcagga tgatgtcgac caggtttttt ccgatgtcgt
     3361 gcacgacgcc cttgacggtg gccagcacga tgcggccctt gcctgcgtcg tcctcggcct
     3421 tctccatgtg tggttcgagg tacgccacag cggttttcat cacttcggcg gactgcagca
     3481 cgaacggcag ttgcatctgg cccgagccga acagctcgcc gacggtcttc atgccggcca
     3541 gcaggtcgtc gttgatgatg gacagtggcg gcttctgcgt catcgcctcg tcgaggtcgg
     3601 cctccagccc gttgcgctcg ccgtcgacga tgcgctgcgc caaccgctcc agcagtggca
     3661 tcttggccag ttcggcggcg cgtgactcct tcgacgacgc cgcggagacg ccctcgaaca
     3721 ttcccatcag ttcctgcagc gggtcgtaac cctcacgccg gcggtcgtac accaggtcca
     3781 aagcgacctc gcgctgcttc tcgtcaatcc tgttcatcgg caggatcttc gaggcgtgca
     3841 cgatcgccga atcgagcccg gcctcctggc attcgtgaag gaagaccgag ttgagcacct
     3901 gccgtgcagc aggattgagg ccgaacgaga tattcgaaag ccccagtgtc gtttggacat
     3961 ccgggtgctt ttgcttcaga agtcggatgg cttcgatggt ttcgatgcca tctttgcgcg
     4021 actcttcctg acccgtgcag atggtgaacg tgagcgtgtc gatcaggatc gaggactcat
     4081 ccacacccca attcccggtg atgtcggcga tgagccgctc ggcgatttcc accttcttct
     4141 cggcggtgcg ggcctgaccc tcctcgtcga ttgtcagcgc gaccaccgcg gcgccgtgct
     4201 cggcgacgag ttccatcgtc ttgtggaacc gcgactccgg cccgtcgccg tcctcgtagt
     4261 tcaccgagtt gatcgcgcac cggccaccga gatgctccaa acccgccttc agcaccgggg
     4321 tttcggtgga gtccagcatg atcggcagcg tcgacgcggt ggccagccgg ctggcaagcg
     4381 cggtcatgtc cgccacgccg tcgcgaccca cgtagtccac gcataggtcg agcaggtgcg
     4441 cgccgtcgcg tgtctgctcc ttggcgatgt cgaggcactt ctgccagtcc tcggcgatca
     4501 tcgcctcacg aaacgccttg gagccgttgg cgttcgtccg ctcgccgatc atcagcaccg
     4561 acgcgtcctg cgcgaacggc gtcgacgagt acagcgacgc gacggcgggc tcgtaggtgg
     4621 cgtggcgctc ggcgcgctgc acattcggaa tcgcgtcggc gaccttgcgg atgtgttcgg
     4681 gtgtggtgcc gcagcacccg ccgaccagcg acagtccata ttcggagacg aagcccgaca
     4741 gcgcctcggc gagttcgtct ggcgtaagcg ggtattcggc accgttggca cccaacaccg
     4801 gcaggcccgc gttcggcatc accgacaccg gggtgcgggc atgttgagac agatagcgca
     4861 ggtgctcgct catctcggcg ggacccgtgg cgcagttcag cccgatcatg tcgacaccga
     4921 gcggctcgac cgccgtcagc gccgcgccga tctcgctgcc cagcagcatg gtgccggtgg
     4981 tctcgacggt cacgtgggcg atcaccggga tgtgacggcc gagctgggcc atcgcccggc
     5041 gtgacccgag caccgcggcc ttcagctgca gcaggtcctg gcatgtctcg acgaggatcg
     5101 cgtccgcgcc gccctccaac atgccgaggg cagcctcggc gtacgcgtcg cggatcaccg
     5161 cgtactcggt gtggccaagc gttggcaact tggtgccggg gcccatcgag cccaacacgt
     5221 accgcctgcg atccggggtg ctcatctcgt cggccacccg ccgcgcgatc gcggtgccgc
     5281 gctgggagag atcacggatt cgatccgcga tgtcgtagtc gccgaggttg gacaggttgc
     5341 agccgaacgt attggtctcg acggcgtccg cgccggcctc gaagtaggcg cggtggatgg
     5401 tctcgatcac atccggacgg gtgtcgttga ggatctcgtt gcagccctcg aggttgttga
     5461 agtcgtcgag cgtgagatcg gccgcctgca gctgcgtgcc catggcgccg tcgcccacca
     5521 tgacccgctt cgacaaggtg tcgagcaaat cggtgtcgta cgcgcgatcg ttcacaacag
     5581 tcacagagac agggtagtcg ctctatccaa atcggcctgt gtggcgatgc cactcatcgc
     5641 tgccacacaa ggcttacgct ggcctagtga ccccgtcgga tgcgagcggc cataggccgg
     5701 ccgacctgcc aaaactcaac gacacgatcg tcgtcgccgc cttcgaaggc tggaacgacg
     5761 cgggcgacgc cgccagcgat gcgctggagc acctggatgc gatctgggaa gcggagccga
     5821 tcgttgagat cgatgacgag gcctattacg actaccaggt gaacaggccc gtgatcagac
     5881 aggtcgacgg cgttacccgg gaactggtgt ggccgtcgat gcggatctca cactgccgcc
     5941 cgcccggctc cgaccgcgac atcgtgctga tgcacggcgt ggagccgaac atgcgctggc
     6001 gcacgttctg cgccgagctg ctcgcgatcg ccgacaagct caacgtgcag accgtcgtca
     6061 tcctcggcgc gctgctggcc gacacgccgc acacccgacc cgtgccggtc tcaggagcgg
     6121 cctactcccc cgactcggcc aagacgttcg gcctggaaga aacccgctac gagggcccaa
     6181 ccggcattgc cggggtgttc caggacgcgt gtgtgcaagc gggcatccct gcggtgacgt
     6241 tctgggcggc cgttccgcac tacgtgtcgc aaccgccgaa cccgaaggcg acggtggcgt
     6301 tgttgcgtcg cgtcgaggat gtcctcgata tagaggtacc gctggccgat ctgcccagcc
     6361 aggccgagga gtgggaacag gccgtgagcg agatgacgtc cgaggacgac gagatcgccg
     6421 agtacgtcca gtccctcgaa gagcgcggcg acgcagaggt cgacatgacc gaggcgctcg
     6481 gcaagatcga tggtgacgcg ctggccgcgg agttc
//
LOCUS       DASRJA010000206        22063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1924330,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000206 DASRJA010000000
VERSION     DASRJA010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22063
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924330"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VFC01_09175"
     CDS             complement(<1..946)
                     /locus_tag="VFC01_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014876858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleandomycin glycosyltransferase"
                     /protein_id="HZN79842.1"
                     /translation="MHIAVVASSAPSHMHPHLAVVRELVDRGHRVSDLVGGHLADLPL
                     PTGADVIGYNSLLPGSPSAPASWPDDDTAGMRMFLDDQIHVLPQVRAALDADPPDIVL
                     YDIGGMAGPVAADLWGVASAQLSPSAVAWDGYHEDMAEVLEPIYGSPAGQKYLRDFDA
                     WLASAGTSLTFDDVTGVPRRCLVLIPRVMQPNSDRVDAVRYRFVGPCIDPRRENADDW
                     TPPACDGPLALMAFGTAYTDRIDLYRNVIGALDDTGWRLVIATGRVAPDELGDVPGWV
                     QVRKAVPQPAVLRVADAFVTHAGMGSCTEGLWYGVPMLA"
     gene            1059..3605
                     /gene="clpB"
                     /locus_tag="VFC01_09180"
     CDS             1059..3605
                     /gene="clpB"
                     /locus_tag="VFC01_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HZN79843.1"
                     /translation="MDSFNPTTKTQAALTASLQAATAAGNPRITPAHLLMALLTQNDG
                     IAAPLLEAVGVEPATIRAEAQRLLDRLPSASGAASQPQLAPQSLAAITTAQNLATEMD
                     DEYVSTEHLMVGLATGDSDVAKLLTGHGASPQALRDAFVKVRGSARVTSPEPEATYQA
                     LEKYSTDLTERAREGKLDPVIGRDNEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVAGDVPESLRDKTVISLDLGSMVAGSKYRGEFEERLKAVLDDIKNSAGQIITF
                     IDELHTIVGAGATGESSMDAGNMIKPMLARGELRLVGATTLDEYRKYIEKDAALERRF
                     QQVFVGEPSVEDTVGILRGLKDRYEVHHGVRITDSALVAAATLSDRYITARFLPDKAI
                     DLVDEAASRLRMEIDSRPVEIDEVERLVRRLEIEEMALAKEEDAASAERLEKLRAELA
                     DQKEKLAELTTRWQNEKNAIDIVRELKEQLETLRGEADRAERDGNLEKASELRYGRIP
                     EIEKKLDAALPQAEAREDVMLKEEVGPDDIADVVSAWTGIPAGRMLEGETAKLLRMED
                     ELGKRVVGQKKAVQAVSDAVRRSRAGVADPNRPTGSFMFLGPTGVGKTELAKALAEFL
                     FDDERAMVRIDMSEYGEKHSVARLVGAPPGYIGYDQGGQLTEAVRRRPYTVILFDEIE
                     KAHPDVFDVLLAVLDEGRLTDGQGRTVDFRNTILILTSNLGAGGSEEQVMAAVRAAFK
                     PEFINRLDDVIIFDGLNPEELVEIVDIQLAQLDKRLAQRRLTLEVSLPAKKWLAERGW
                     DPQYGARPLRRLIQQAIGDQLAKMLLAGEVHDGDVVPVNVSADGDSLVLG"
     gene            complement(3744..5084)
                     /locus_tag="VFC01_09185"
     CDS             complement(3744..5084)
                     /locus_tag="VFC01_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79844.1"
                     /translation="MLTEVEPVDIEERLADPKTYEDTILRIFTKRSERGSGFSEAHCG
                     VSYFSTAAERQGLAKAIARSVTDGEFRPRPVDLWILETNGKRREAHLADFPDHVVGSA
                     LFKLLTHNARCYGLPGVYSYLSGVTNVVAMQTFAAFIRAHRDRVGVKGPPLYVLQSDF
                     EKYGDNLPVGRDAALWRILKDVAALGSPNGEIGSTTWDLITGLVRPVVRETDGTEFTR
                     LKGVAMGTPLVPLLGNLAVAPMDAAITSIDGIFYARYNDDFIVAHSDLAALHEADARI
                     DSLIDELGVKRKLSKELRTALSGTGQPSTRDPAYRGRDRIDCLGLTVSHAGTLSLGPH
                     RLSRFIARVARRLDAAVPALSQASVDERARHLVAATNVMLDVTSPFAVAGLSSILDTT
                     TDRGSLKDLDFRIARKIVQVATRRSGVRGFRQLPPAVLYRDMGLVSLVHLRNLR"
     gene            complement(5074..7143)
                     /locus_tag="VFC01_09190"
     CDS             complement(5074..7143)
                     /locus_tag="VFC01_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79845.1"
                     /translation="MNDIDRWFEKAARKENRSVSLYASLLTGRVVAYFVYRMRYALLL
                     DGARYAIHVAEFLIILTSLGGLAAFTVMMLRVGSLIISGAWWGLLEVMRERLRALAQA
                     GDRDAIEREIGSWLTLSAVVATVVTVGGTAALVLVLPSDHDPMGSFYAFLILIELALR
                     MPVRVLHSGMFATRRIYRPLWSMFAPTALSVLVIAVGVFLYPTAAIVIAIILSNALSI
                     WISVHYTVRAYRLTGLWPQHGGRSLRRVLPSIPPRLGIEATLSGLGLRLDAVAVLLIA
                     GMYSMNNRAFDLTAGYSAWRNVDPFQFFYLVLPLLRGSYEATAVFYFDFVRLRRIPAF
                     RQFRMRFFYDLLWTTPIITLFFWSLSIPLGLLVLPGIPFSFLLALLPLFVVRSLIGAY
                     QIRLFAEGQFRALNATILFSAGLFALVWFDVNPASDLVQITAAMIVLLIVHINLQHLQ
                     DRVTVLPTLLSLPDWIRALTAESGPVQVGRISVPDWIDSRQKANAITLMRETFGSEGH
                     LAFRSSSNLVFYERMTTKNTGSQPHLALQTATGGAANRAEILPTAMENGDQALDRIVS
                     EEWMAPIDDALSNNTEVLESDFRAIFPDGIVVDLQKREGRRVARGLEQNLLVGLLPMA
                     MKSLDQGAVVTSVSGRWVSPIFYGGGLRLIFILPSEVDSDRFRRWRRTVNTWHAGSRL
                     SEVSHAH"
     gene            7397..8065
                     /locus_tag="VFC01_09195"
     CDS             7397..8065
                     /locus_tag="VFC01_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T(2)"
                     /protein_id="HZN79846.1"
                     /translation="MEFEPVDTSSTWLVMTWGPSLCSAEPTSPGCKSGRVEKMGPTWI
                     LHGLWPQPAANQYCGVPKGLLDRARDIRRSDLPPVDIGDDVRASLQSMMSDASIMGPH
                     EWYAHGTCSGVTPDVFFGDAVTLVEQARTVLDPMFADAEEGRIQLSDVQDKLNQVYGA
                     GAGDRVRLSCRNMTGVGSVAYELHLSLPPVPDLRAVDGTLSLKDLIVKAPPLAPGCLH
                     ASVP"
     gene            8158..9000
                     /gene="ttfA"
                     /locus_tag="VFC01_09200"
     CDS             8158..9000
                     /gene="ttfA"
                     /locus_tag="VFC01_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose monomycolate transport factor TtfA"
                     /protein_id="HZN79847.1"
                     /translation="MVPLWFTLSALCFVGAAVLLYVDIDRRRGLGRRRKSWAKSHGFD
                     YEHESADILKRWKRGVMSTVGDITAKNVVLGQIRGEAVFIFDLEDVATVIALHRKVGT
                     NVVVDLRLKGIKEPRENDIWLLGAIGPRMVYSTNLDAARRACDRRMVTFAHTAPDCAE
                     IMWNEQNWTLVSMPVTSTRAQWDEGLRTVRQFNDLLRVLPPLPQATTGQPVARRGGSP
                     SRPLSPAPARSELPTGRADMPPGRADVGRYTQARPEPGRSEPVRRPPPPPSRNGRQSP
                     HYQR"
     gene            9015..9791
                     /locus_tag="VFC01_09205"
     CDS             9015..9791
                     /locus_tag="VFC01_09205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN79848.1"
                     /translation="MARPVALITGPTSGLGAGFARRYAIDGYDLVLVARDAERLERLA
                     AELHDEAGAEVEVLVADLAVEADRAKVADRLAAGVRVLVNNAGFGTSGEFWTADLALL
                     QSQLDVNVTAVMQLTHAALPPMLSAHEGTVINVASVAGLLPGRGSTYSASKAWVIAFS
                     EGLANGLTGTGVGVHALCPGFVHTEFHARAGIEMSGTPSFLWLEVDDVVRETMADVAK
                     GKVVMVPGLQYKALTTGGRMVPRNLVRAVSKVVGKGRGRT"
     gene            9792..10691
                     /locus_tag="VFC01_09210"
     CDS             9792..10691
                     /locus_tag="VFC01_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79849.1"
                     /translation="MSPRALVAVVAAIIMVASACSGDDGHPNPYSTQSAAIGESLSIL
                     GWNISVTNLRFDSEHVLVDVDASPSQKDAPHAKPEDIRFGLYGALAHPIEANALGSCK
                     DVTSLALQPVSAPSPDRLTGTVCLGPQRDQSQVRGVYAYSPRERIPDTAAAYPAAFPV
                     GVPPTNENDTGLMLRTTSVDAFSADGAQLDPSAVGDPNAFSGNGYMLLGLEIEGLAAR
                     YRDDSARRGGPMMVLAAPTLPAPGLSHACDIYGASVLVLPDASRDAVQVRVSLCTQGE
                     INSALLYATVSMVGTHAGLWEKG"
     gene            10688..11359
                     /locus_tag="VFC01_09215"
     CDS             10688..11359
                     /locus_tag="VFC01_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZN79850.1"
                     /translation="MTGHRPDVAAAAAPGPTEWGDTPGVGPWEGPHPEDPRYDPALLR
                     DGDTRNVVDAYRYWTRVAIIADIDQRRHPLHIAIENFGNDANIGGVVRTANAFAVDTV
                     HIVGRRRWNRRGAMVTDRYQRLRHHDTTAELLTFAAAAGLTVVAVDNIPGAVRIEHTP
                     LPRACLLIFGQEGPGITDDAKAGAAVTVSIAQFGSTRSINAAVAAGIAMHAWITQHGD
                     ISKAW"
     gene            complement(11411..12589)
                     /locus_tag="VFC01_09220"
     CDS             complement(11411..12589)
                     /locus_tag="VFC01_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN79851.1"
                     /translation="MRRTYKFRMRPTVRQHLALATCLESHRELYNAALQERKDAWRHS
                     RTAIDYRTQSGQLKDIRRDRPDVAIWSFSSQQATLRRLNRAFDGFFRRVKDGAAPGYP
                     RFKGRGRFDSVEWPKDGDGARWHPDTNRVYLRGIGQVKVTAHRQVVGLVKTIQIRREG
                     RRWALILSCDDVPVDPLPGTGRQVGVDLGIASFATTSDGVHISNPRWERRAAARLTRA
                     QVRLARAKRASNNRRNRRETAAARHRKIANQRRDFHHKTARALVARYDLIVVEDLAIT
                     NMRRRAKPLPDPDKPGAFLQNRAAAKTGLNRSISDAAWGQFVSILRAKAEDAGRRWIE
                     VDPKHTSDGCENCGYAARGNRVNQAVFACQACGHTAQADEHAARNILRAGLALHAHAA
                     "
     gene            12928..14055
                     /locus_tag="VFC01_09225"
     CDS             12928..14055
                     /locus_tag="VFC01_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HZN79852.1"
                     /translation="MDQVWANRAASAEAAIAKRHLKRLWGLPGTQLGVVAWPAARKYR
                     NFDTWHYWWQAHLLDNLVDAHVRDPQPDRLKQIARQIRGHRLRNNGSWVNDYYDDMAW
                     LALALERAGRLAGVETPGALKKLSEQFLNAWVPEDGGGIPWRKQDQFFNAPANGPAAI
                     FLARYEDRLRRAQQMSDWIDETLIDPETHLVFDGIKAGSLVRAQYTYCQGVVIGLDVE
                     LASRTKDPEHAARVHRLVKAVRDNMAPEGVIKGAGGGDGGLFNGVLARYLALVVTTLP
                     QNGPADAEARDIARSLVVKSAESAWENRQTVDGLPLFAAFWDRTAEMPTAGGQEAQFV
                     EGAVNASAVPERDLSVQLSGWMLMEAAHAVTAASGDTGTDE"
     gene            14052..15899
                     /gene="nhaA"
                     /locus_tag="VFC01_09230"
     CDS             14052..15899
                     /gene="nhaA"
                     /locus_tag="VFC01_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HZN79853.1"
                     /translation="MTAEPLPRIVRLLPGRFSRDPKAPGTKEKCAAGLLLACTVAAIL
                     WANSPWAQTYWALLDTHVGIMFAGHTFELTVQHLINDGLMTFFFFIVGLEVTSEFAIG
                     GLTDRARAAVPVVAAIAGLTVPAVIFLLFNASGENARAWGVVISTDTAFLIGALAIIK
                     PKYPARLRIFLLTLAVVDDIGALCVIALFYSERIQVAPLVVAVALIVAIALVRFLPAA
                     RGPAYAVLGFALWVALSAAGIHPTLAGVAVALLIPVFTPDRRQVEQTVDMIRAFRQSP
                     NSQYARAATRGLRESISINERLQAEIGPYVSFVVLPLFALANAGVRLDMDSVSAAMRS
                     PLTWGIVVGLVVGKFIGITGATWIMQRTGLGQLAPGLTLRRVAGGAALSGIGFTIALF
                     IVDIAISDPGRQDQARIGVLLASVVAFLAGWGIFRITDRISPPEPVGLKLIRPIDPDR
                     DHIRGDPNAPLTLVEYGDYECPFCSRATGGIDEVLERLGDDLRYVWRHLPLERVHPRA
                     FDAARAAEAAGLQGKYFEMGRMLFAHQDDLEWSDIYRYANAIGLDLERFDQDVRVHPT
                     KVLQRVQDDAQDAELMDLNSTPTFFVNGKRHKGPWDAASLIRALEAGRI"
     gene            complement(16041..16796)
                     /locus_tag="VFC01_09235"
     CDS             complement(16041..16796)
                     /locus_tag="VFC01_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79854.1"
                     /translation="MFVALISITLACIACSLWVRRHTWLSRWEVAGTLCIAFQGCAAL
                     LISPTASEFLGPPLHAISGMRNVEDYLGHVSYLLAAVALVQTVLSRVIDDDRLQAIGT
                     QWVERPATVCIPLMFAAWASGNGSKVYRSNFYEVPGDAWSKIYWFLFCAMLIYLLAWT
                     LCGLLVLRRIPRHRPTATVYLVLVVGGIAACTARIVTQWCSWSDRGAVGWLSTFAGGI
                     IFAVAAARSWRNKTRWFKRRPDPGSPHPTPAGR"
     gene            complement(16836..17198)
                     /locus_tag="VFC01_09240"
     CDS             complement(16836..17198)
                     /locus_tag="VFC01_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN79855.1"
                     /translation="MHEYRRFVQAELDARGWTPADLVHRSGLSRQLVWKILHDDRPTL
                     GQMPDESTLEGIANGFGVSVERVRVAAARSLRGYEDDDTPLITDLREVSTDALLHEIR
                     RRVAGGDEWRDREDAPPL"
     gene            17465..19726
                     /locus_tag="VFC01_09245"
     CDS             17465..19726
                     /locus_tag="VFC01_09245"
                     /inference="COORDINATES: protein motif:HMM:NF019842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HZN79856.1"
                     /translation="MSNKAVRAKKRRAARAKRVAMMGAVSATAVAVGLTPATANAASS
                     QTYFIGFPDWLPIGEGATLPTDPVAINNAMVGAKDSNPLIGWGTGGVNLQPVWVRWVD
                     GVPQYTLPVVGQTGSHPETNPAYQPAYDAAYWAAYNLWSWLGGCQGRSNRAQCATNAA
                     LSAVSGIPQTITVPDYGVLEDGYWTTTTAGQWVTSSDVGDLPTAAQLAYAAYVLQNGD
                     LSALAPLLNWTAYFTNVNLIAYGDGAIAAGLAYQAFIDSAQGETHEGYDPYAVGAALT
                     GPRKIVIIDPTGKVTLISVDETNNPLDLPEIIYPGSGEMPDYESAQDGGVLDLTALSL
                     VLVRNPGRANGGLYARFAPIYQELTGVNPVTPERQDVLPAGVDPALITKLLTGNAGDI
                     SLNDVGDLQAVIESADGKPIIVTLKADIGWEYDLLSDAPATANPIAWANSVASSIFLT
                     NLLTGVDFQDLGDGASIGPDGTLYYTLPVDELPLLAPMRLPAQAIGLVTGNPDVNTPL
                     ADAIEPALKMLVNLGYTDVVRNPDGTYTRTLDRFGEPTLFGTPTLTREQMALVPGDLI
                     AALGRGFGDEMTDVLLRTKGQLTEALKVEMTDEQDEAVEQALSAPGTAIKNASHDVGN
                     GVSQVLTAVESELPEAPAVTQDDLEEGQRKVGRQLADTRDQINETVTKVQSVIGNGRN
                     TATTANTSDGGENGDTPSTTRKTPVKDALQKANSDIKKTVTSVRDQVKKAFGGEKDSD
                     GSGNGDGGEGGAQ"
     gene            complement(19793..20758)
                     /locus_tag="VFC01_09250"
     CDS             complement(19793..20758)
                     /locus_tag="VFC01_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN79857.1"
                     /translation="MTPAQLRAFSAVVRLGSVHGAAEELGMSDAGISMHVAQLRKELD
                     DQLFSRTSAGLAFTPGGLRLASRAVEILGLQQQTAIEVTEAAHGRRLLRIAASSAFAE
                     HAAPGLIELFSSRANDLSVELSVHPTSRFKELISSRAVDIALGQASDDRQATDNAIVV
                     RPFLNYQILTVAAPGSPLITHPPTPALLREQLWMLGPSAGSVDGEIAAVVRNLAIPES
                     HQRIFQSDAAALEEVQRVGGVSLAIGFTVGKDLAAGRLIHVKGQGLQVPGEWCAFTLT
                     PAARQPAVSELVRFITTPRCTQAMLRGTGVGVTRFRPKIHVTLWS"
     gene            20866..21756
                     /locus_tag="VFC01_09255"
     CDS             20866..21756
                     /locus_tag="VFC01_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZN79858.1"
                     /translation="MQVPGPFEYERATSVDHAIGLLDRLGDGAMLVAGGHSLLPMMKL
                     RIANPEYLVDINDLIPELGYIVVDPTLVRIGAMTRHREALESEDLAAVCPIFRDAERV
                     IADPVVRNRGTVGGSLCQADPAEDLTTVCTVLNAVCLARGPSGEREISIDDFLTGPYE
                     TSLAHNEMLIEIRIPLRHNASSAYAKVERRVGDWAVAAAGAAVRLDGDAIVAARLGLT
                     AVNTDAAALAAVGGGLAGRPATEETFAQAGRDAAQACSPVTDMRGSAEYKRHLASELT
                     VRTLRTAVERVRSATAVEGN"
     gene            21759..>22063
                     /locus_tag="VFC01_09260"
     CDS             21759..>22063
                     /locus_tag="VFC01_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZN79859.1"
                     /translation="MQVNMTVNGEPVTAEVEPRTLLVHFLRDQLRLTGTHWGCDTSNC
                     GTCVVEMDGEPVKSCTVLAAMAAGRSILTVEGLEVDGHLDPVQEGFMRCHGLQCGFCT
                     "
BASE COUNT         4070 a         7056 c         7275 g         3662 t
ORIGIN      
        1 tggcgagcat cggcacgccg taccagagtc cctcggtgca cgagcccata cccgcgtgcg
       61 tcacgaacgc gtcggccacc cggagcaccg ccggctgcgg cacggccttt cgcacctgca
      121 cccagccagg cacgtcgccc agctcgtcgg gtgccacccg gcccgtcgca atgacgaggc
      181 gccaaccggt gtcatcgagc gccccaatca cattgcggta caggtcgatt cggtcggtgt
      241 atgcggtgcc gaacgccatc agggcgagag gaccgtcgca ggcgggtggc gtccaatcgt
      301 cggcgttttc tcgccgcgga tcgatgcacg gtccgacgaa ccggtagcgg acagcgtcga
      361 cacgatcgga gttgggctgc atgacacggg gaatcagcac gagacagcgg cggggcacgc
      421 ccgttacgtc gtcgaaggtc agcgaggtgc cggcggaggc cagccacgcg tcgaagtcac
      481 gcagatactt ctggccggcg ggactgccgt agatcggctc tagcacctcg gccatgtcct
      541 cgtggtagcc gtcccatgcg accgcgctcg gcgacagttg cgccgacgca acgccccaga
      601 ggtcggccgc caccggcccg gccatgccgc cgatgtcgta gagcacaatg tcgggtgggt
      661 cggcatccag ggctgctcga acctgcggca gcacgtgaat ctgatcgtcg aggaacatcc
      721 gcatgcccgc ggtgtcgtcg tcgggccagc tggccggtgc gctcggcgag ccgggcagca
      781 gcgagttgta gccgatgacg tccgcgccgg tcggaagggg cagatcggca agatggccgc
      841 cgaccagatc gctgacgcgg tggccgcggt cgaccagttc acggacgacg gcgaggtggg
      901 ggtgcatgtg gctgggcgcg gaggaggcga cgacggcgat atgcatcccg tcccattgtc
      961 cggcggtcgg aaaaattgtc agacttgagc ggaacagact caaccttgac gacgttgaac
     1021 atcgcgacaa gcattttctc tatacgaacg gaggtgtcgt ggactcgttc aacccgacga
     1081 ccaagaccca ggcggcgctg acggcgtcgt tgcaggcggc gaccgccgca ggcaacccgc
     1141 ggatcacgcc ggcccacctt ttgatggcat tgctcaccca gaacgatggc atcgccgccc
     1201 cattattgga ggccgtcggc gtcgagccag cgacaattcg cgccgaggcg caacgcctcc
     1261 tcgaccggct gcccagcgcc agcggcgcag cctcgcagcc gcagctggcc ccgcagtcgc
     1321 tggccgcgat caccacggcg cagaacctcg ccacggagat ggacgatgag tacgtctcga
     1381 ccgagcacct gatggtcggc ctggccaccg gcgactccga cgtggccaag ctgctgaccg
     1441 gtcatggcgc gtcacctcaa gctttaaggg atgcgtttgt caaggtgcgc ggcagcgccc
     1501 gcgtcacgag cccggagccc gaggcgacat atcaggcgtt ggagaagtac tccaccgacc
     1561 tgaccgagcg tgcccgcgag ggcaagctcg atccagtcat tgggcgcgac aacgagattc
     1621 gtcgcgtcgt gcaggtgctc tcgaggcgca ccaagaacaa cccggtgctg atcggcgagc
     1681 ccggtgtcgg caagaccgcg atcgtggagg gcctggccca gcgcatcgtg gcgggcgacg
     1741 tgccggagag cctgcgcgac aagaccgtta tcagcttgga cctcggttcg atggtcgccg
     1801 ggtcgaaata tcgcggcgag ttcgaggagc ggctcaaggc cgtgctcgac gacatcaaga
     1861 actcggccgg ccagatcatc acgttcatcg acgagctgca caccatcgtg ggcgccggtg
     1921 cgaccggcga gtcctcgatg gacgccggca acatgatcaa gccgatgctg gcccgcggcg
     1981 agctgcgcct ggtcggtgcc accaccctcg acgagtaccg caaatacatc gagaaggacg
     2041 ccgcgctgga gcgccgcttc cagcaggtgt tcgtcggcga gccgtcggtc gaggacaccg
     2101 taggcatcct gcgcggcctg aaggaccgct acgaggtgca ccacggtgtg cgcatcacgg
     2161 actccgcgct ggtcgcggcg gccacgctgt ccgaccgcta catcaccgcg cggttcctgc
     2221 cggacaaggc catcgacctg gtcgacgagg ccgccagccg gttgaggatg gagatcgact
     2281 cgcggcccgt cgagatcgac gaggtcgagc ggttggtccg tcgcctcgag atcgaggaga
     2341 tggcgctggc caaagaggaa gacgcggcgt cggcggaacg gctggagaag ctgcgcgccg
     2401 agctggccga ccagaaagaa aagctcgcgg agctcacgac gcgatggcag aacgagaaga
     2461 acgccatcga catcgtccgc gagctcaagg agcagctgga gacgctgcgc ggcgaggccg
     2521 accgagccga gcgcgacggc aacctggaga aggcgtccga gctgcgctac ggccgcatcc
     2581 ccgagatcga gaagaagctc gatgccgcac tcccgcaggc cgaggcccgc gaggacgtca
     2641 tgctcaagga agaggtcgga cccgacgaca tcgccgacgt ggtgtcggcg tggaccggta
     2701 ttccggctgg gcggatgctc gagggcgaga ccgccaagct gctgcgcatg gaagacgagc
     2761 tgggcaagcg cgtcgtcggc cagaagaagg ccgtgcaggc ggtgtcggat gcggtgcgcc
     2821 gcagccgggc cggcgtcgcg gatccgaacc ggccgacggg ctcgttcatg ttcctcggcc
     2881 cgacaggtgt cggtaagacc gaactcgcaa aagcgttggc ggagttcctg tttgacgacg
     2941 aacgcgcgat ggtccgcatc gacatgagcg agtacggcga gaagcactcg gtggcccggc
     3001 tggtcggtgc gcctcccggc tacatcggct acgaccaggg cggtcagctg accgaggcgg
     3061 tgcggcggcg tccgtacacc gtgatcctgt tcgacgagat cgagaaagcg catccggacg
     3121 tgttcgacgt gctgttggct gtgctcgacg aaggccggct gacggacggt cagggccgca
     3181 cggtcgattt ccgcaacacg atcctgatcc tgacgtccaa cctgggtgca ggtgggtcgg
     3241 aagagcaggt gatggcggcg gtgcgcgcgg cgttcaagcc ggagttcatc aaccggctcg
     3301 acgacgtgat catcttcgac gggctcaacc ccgaggagct ggtcgagatc gtcgacatcc
     3361 agctggcgca gctggacaag cggttggcgc agcgcaggct gacgctcgag gtgtcgctgc
     3421 ccgccaagaa gtggctggcc gaacgcgggt gggacccgca atacggtgcc cgtccgctgc
     3481 gacggctgat tcagcaggcc atcggcgacc agctggccaa gatgctgctg gcaggggagg
     3541 tgcatgacgg cgacgtcgtg ccggtcaacg tcagcgcgga cggcgactcg ctggtcctcg
     3601 gatgagcgct cgcgcggaag tgtgaaggcg cggggtaaaa cgccaaaatt gaagggccga
     3661 aagccgcaac ctctatagcc cttcgcggtg accgcccgaa ggcgggatcc gcgccttgct
     3721 gaactgtagg tgcgcggtgc aagtcaccgc aagttgcgca aatgcactag cgacaccaat
     3781 cccatgtcgc gatacagcac ggcgggcggc agctgccgaa agccgcgaac ccccgacctc
     3841 cgcgtggcca cctgcacgat cttgcgcgcg atccggaagt ccagatcctt cagcgatccc
     3901 cgatccgtgg tcgtgtcgag gatcgaggac aagcccgcga cagcgaaggg gctcgtcacg
     3961 tcgagcatga cgttggtggc cgcgacgagg tgacgtgctc gctcgtcgac ggacgcttgc
     4021 gatagcgccg gtacggcggc gtcgaggcgt cgcgctacgc gggcgatgaa gcggctcagc
     4081 ctgtgtggac caaggctgag cgtgccggcg tggctgacgg tcagccccag gcagtcgatg
     4141 cggtcgcggc cgcgataggc gggatcccgc gtcgacggtt gaccggttcc gctcagtgcg
     4201 gtgcgaagct ccttcgacag cttgcgtttg acgcccaatt cgtcgattag tgaatcgatc
     4261 ctcgcgtccg cctcatgcag ggcggcgaga tccgaatggg cgacgatgaa gtcgtcgttg
     4321 tagcgcgcgt agaagattcc gtcgatcgat gtgatcgccg catccattgg tgcgaccgcg
     4381 agattaccta gcagtgggac cagaggggtt cccatcgcga cacctttgag ccgggtgaac
     4441 tcggtgccgt cggtttcccg cactacggga cgaaccagcc cggtgatgag atcccatgtg
     4501 gtcgagccga tttcgccgtt cgggctgcca agggcggcga cgtctttcag gatgcgccac
     4561 agggccgcgt cacgaccgac cggaagattg tccccgtact tttcgaaatc ggattgcagg
     4621 acgtacaacg gcggcccttt gacgccgacc cggtcgcggt gggcgcggat gaaggcggcg
     4681 aacgtctgca ttgcaacgac attcgtcaca ccggacagat aggagtagac gccgggcaga
     4741 ccataacacc gcgcattgtg cgtgaggagc ttgaagagcg cagagcccac gacgtgatcg
     4801 ggaaagtctg ccaggtgcgc ctcgcgacgt ttaccgttgg tttcaaggat ccagagatcc
     4861 actggccgtg gccggaattc gccgtcagtg acactgcggg cgatggcctt ggccaggccc
     4921 tgccgttcgg ccgcggtact gaaatacgag acaccgcagt gcgcctcact gaatcccgaa
     4981 ccgcgttcgc ttctcttggt gaaaatccgc aggatggtgt cttcgtaggt cttggggtcg
     5041 gccagccgtt cctcgatgtc gaccggttca acctcagtga gcatggctca cctccgaaag
     5101 gcggctgccg gcgtgccagg tgttcacggt tcggcgccag cggcggaacc gatcgctgtc
     5161 gacctcggac ggcaggatga agatcaggcg taatccgcct ccgtagaaga tcggcgagac
     5221 ccagcgccct gacaccgaag tcacgacggc accctggtcg aggctcttca tcgccatagg
     5281 caggagtccc acgaggagat tctgctcgag gccacgtgcg acccgccttc cttcccgctt
     5341 ctgcaaatcc acgacgatgc cgtcgggaaa gatcgcacga aagtctgact cgaggacttc
     5401 cgtgttattc gacaatgcgt catcgattgg tgccatccac tcctctgaga cgatccgatc
     5461 aagcgcctgg tcgccgttct ccatcgctgt cgggaggatt tcggcacgat tcgctgcgcc
     5521 tccggtagca gtttgaagcg ccagatgcgg ttgactccca gtgttcttgg ttgtcatccg
     5581 ctcgtagaac accaggttcg atgacgaccg gaacgccaga tggccttcac tgccgaaggt
     5641 ttcccgcatc agcgtgatcg cgttggcctt ctgccgtgag tcgatccagt cgggcaccga
     5701 gattctgccc acttgcaccg gccccgactc ggcggtgagc gcgcggatcc agtctggaag
     5761 ggacagcagc gtcggcagca cagtcacgcg gtcttgaagg tgctgcagat tgatgtgcac
     5821 gatgagaagc acgatcatgg ccgcggtgat ctggacgaga tccgaggctg ggttgacgtc
     5881 gaaccacacg agggcgaaca gccccgctga gaacaagatg gtcgcgttca gggcgcggaa
     5941 ctggccttcg gcgaacaagc ggatctggta tgcgccgatc aagctgcgaa caacgaagag
     6001 cggcaggagc gccagcagga agctgaacgg tatcccgggc agaaccaaga gtcccaacgg
     6061 aattgagagc gaccagaaga acagggtgat gatcggcgtg gtccacaaca aatcgtagaa
     6121 gaaccgcata cggaactgac gaaatgccgg aatgcgtcgc aggcgaacga agtcgaagta
     6181 gaaaacggcc gtcgcctcat atgagccacg taacagcggc agcaccagat agaagaattg
     6241 aaagggatcg acattgcgcc atgccgaata gccggcggtg agatcgaaag cccggttgtt
     6301 catcgaatac atgccggcga tcagcagaac cgccaccgca tccagtcgca gacccagacc
     6361 cgagagggtt gcctcgatgc caagtcgagg cgggatcgac gggagtaccc gccgcaatga
     6421 acgaccgcca tgttgcggcc acagcccggt aagccggtag gcgcgcaccg tgtagtgaac
     6481 cgagatccag atgctgagcg cgttcgacaa gatgatcgcg atgacaattg cggcggtcgg
     6541 atagagaaag acgccaacgg cgatcacgag aactgatagg gcagtcggcg cgaacatgga
     6601 ccacaaaggt cggtagatgc gccgggtggc gaacatgccg gaatgaagca cccgcacggg
     6661 catgcgcaag gcgagttcga tgaggatgag aaacgcgtag aaggacccca tcggatcgtg
     6721 gtcggagggg agcacaagga ccagggccgc tgtgccgccg actgtcacaa ccgttgccac
     6781 gactgccgat agcgtgagcc aactgccgat ctcgcgctcg attgcgtccc ggtccccggc
     6841 ctgagccagg gctctgagtc gctcgcgcat cacttcgagt agcccccacc acgcgccgct
     6901 gatgatgagg ctgcccactc gcagcatcat gacggtgaac gccgccagcc caccgaggct
     6961 ggtgaggatg atcaggaatt cggcgacgtg aatcgcgtag cgggctccgt cgagcagcag
     7021 cgcatagcgc atgcggtaga cgaagtacgc gacgaccctg ccggtgagaa ggctcgcgta
     7081 cagcgagaca gaacgattct ccttgcgcgc cgccttttcg aaccaccggt ctatatcgtt
     7141 catagatgcg ctcgtcggtg gcggcaaaac tcgtgtggcc tttcgctagc gaacaaatca
     7201 cggggaccgg tgcggggtgg gaagctgtag tccgttgcgc atcacggcga gcccggaagt
     7261 tcgctctccc aatgtagaag ggtgcaccat gcggcgaggc gatgtagccg tattgtcgat
     7321 ttcctgcgtt ctagcagcaa ttgtcgtcgc ggccatcacc ttcagcgttc gcgttttgga
     7381 ccgcacgccg gtcaccgtgg aattcgaacc tgtcgacacc agctcgacct ggctcgtcat
     7441 gacgtggggc ccgagtctgt gcagcgctga gccgaccagt ccgggttgca agtccggtcg
     7501 ggtggagaag atggggccca cctggatcct gcacgggctg tggccgcagc cggccgccaa
     7561 tcagtactgc ggcgtcccga agggcctcct cgaccgcgcc cgcgacatcc gcaggagcga
     7621 cttgccgccc gtggatatcg gggatgacgt gcgggcctct cttcagtcga tgatgtcgga
     7681 cgcttcgatc atgggtcccc acgagtggta cgcgcacggc acctgctccg gcgtgacacc
     7741 ggatgtgttc ttcggcgacg ctgtgacact cgtcgagcag gctcgtacgg ttctcgatcc
     7801 gatgttcgca gatgccgagg aaggccggat acagctgagc gacgtgcagg acaagctcaa
     7861 tcaggtgtac ggcgccggag cgggcgatcg cgtccgtctc agttgtcgca atatgaccgg
     7921 agtcggtagc gttgcttacg agctacacct gtcgcttccc ccggtccccg atctgagggc
     7981 cgtcgacggg acgctgtcgc tgaaagacct gatcgtcaag gccccaccgc tcgcgcccgg
     8041 gtgcttgcac gcgagcgtgc cctgaccggc ccgttggtcc cggccaaacg gtatgtgacc
     8101 cggttgtgac gtccttgcgt tcgggtataa gaggtaccag caagtaggct aggcgcgatg
     8161 gttccccttt ggttcacgct gtctgcactc tgtttcgtgg gtgcggcggt gctgctgtac
     8221 gtcgacatcg accgtcgacg tgggctgggc cgtcggcgga agtcgtgggc gaagtcacac
     8281 ggcttcgact acgagcacga atcggccgac attctcaagc gctggaagcg cggcgtgatg
     8341 tcgaccgtcg gcgatatcac cgccaagaat gtcgtgctcg ggcaaatccg gggcgaggcg
     8401 gtgttcatct tcgacctcga agacgtcgcg acggtcatcg cgctgcaccg caaggtcggc
     8461 accaacgtcg tcgtcgacct gcggctcaag ggcatcaagg agccgcggga gaacgacatc
     8521 tggctgctcg gcgcgatagg cccgcggatg gtgtactcca ccaacctcga cgccgcgcgc
     8581 cgggcctgcg accggcggat ggtgacgttc gcccacacgg cgccggattg cgccgaaatc
     8641 atgtggaacg aacagaactg gacgctggta tcgatgccgg tcaccagcac ccgcgcccag
     8701 tgggacgaag gcctacggac cgtgcgccag ttcaacgacc tgctgcgcgt gctgccgccg
     8761 ctcccgcagg ccacgacggg ccagcccgtc gcacgccgcg gtggctcgcc aagccggccg
     8821 ctcagccccg cgcccgcccg ctccgaactg ccgaccggcc gggccgatat gccgccgggc
     8881 cgcgccgacg tcggccgcta cacgcaggcg cgccccgagc ccggccgatc ggaaccggtg
     8941 cgccgcccgc cgccaccgcc gtcacgcaac ggccggcagt ctccgcacta ccagcgctaa
     9001 gtagtgtcta gggcatggca cgccccgtcg cattgatcac cggaccgacg tccgggctcg
     9061 gcgcgggatt cgcacgccgc tacgccatcg acggctacga cctggtgctg gtggcccgcg
     9121 acgccgaacg actcgagcgc ctcgccgcgg aactgcatga cgaggcgggc gccgaagtag
     9181 aggttttggt cgccgacctg gccgtcgaag ccgaccgcgc gaaggtggcg gatcgactcg
     9241 ccgccggggt acgggttctg gtgaacaacg cggggtttgg cacctccggc gagttctgga
     9301 ccgccgacct ggcgctgctg cagtcccagc tcgacgtcaa cgtcaccgcg gtgatgcaac
     9361 tgacgcacgc cgcgctgccg cccatgcttt ctgcccacga agggacggtg atcaacgtcg
     9421 ccagtgtggc ggggttgctg cccggccgcg gatcgacgta ctcggcatcc aaggcgtggg
     9481 tgatcgcgtt ctccgaaggg ctcgccaacg ggctcaccgg caccggtgtc ggggtgcacg
     9541 cgttgtgtcc cggcttcgtg cacaccgagt ttcacgcccg cgcaggcatc gagatgagcg
     9601 gcaccccgtc attcctctgg ctcgaggtgg acgacgtggt gcgcgaaacg atggccgacg
     9661 tcgccaaagg caaggtggtc atggttcccg gactgcagta caaggcgctc accaccggcg
     9721 gccggatggt gccgcgaaac ctggtgcggg cggtgtcgaa agtcgttggc aagggccgcg
     9781 gccgcaccta gatgtcgccg cgtgccctag tcgccgtcgt cgcggcgatc atcatggtcg
     9841 cctcggcttg ctccggtgac gacggccacc cgaatccgta ctccacgcaa tccgcagcca
     9901 tcggcgaatc gctgtccatc ctcggttgga acatctcggt gactaatctg cggttcgact
     9961 ccgagcatgt cctcgtcgac gtggatgcct cgccttcgca gaaggacgcc ccgcacgcca
    10021 aacccgagga catccggttc gggctctacg gcgcgctggc gcacccgatt gaggcaaatg
    10081 ctcttggcag ctgcaaggac gtgacaagcc ttgccctgca accagtatcg gcgccttctc
    10141 cggaccggct caccggcacg gtctgcttgg gcccacagcg cgaccagtcc caggtgcgcg
    10201 gtgtgtacgc gtactcgccg cgcgaacgta ttccggacac cgcagccgcg tacccagcgg
    10261 cgttccctgt cggcgtgccg ccgacgaacg agaacgacac cgggctgatg ctcaggacaa
    10321 cgagcgttga cgcgttcagt gccgacggcg cgcagctcga cccgtcggcg gtcggcgatc
    10381 caaatgcgtt ttccggcaac ggctatatgc ttctcggcct cgaaatcgaa ggcctcgcag
    10441 cacgataccg tgacgactcg gcccgccgag gcggccccat gatggtgctg gccgcgccga
    10501 cactgcccgc gcctggcctc agccacgcct gcgacatcta cggcgcctcg gtgctcgtct
    10561 tgccggacgc atcgcgcgac gctgtgcagg tccgcgtgtc gctctgcaca cagggggaga
    10621 tcaacagcgc actgctctac gcgacggtgt cgatggtcgg cacccacgcc ggattgtggg
    10681 aaaaaggatg actggacatc gccctgatgt cgccgccgca gcggctccgg gcccgaccga
    10741 atggggcgac acccccggcg tcgggccctg ggaaggtcca caccccgagg atccgcgcta
    10801 cgaccccgcg ctgctgcgcg acggcgacac acgcaacgtc gttgacgcat accggtactg
    10861 gacgcgcgtg gcgatcatcg ccgacatcga ccagcgcagg catccgctgc acatcgcgat
    10921 cgagaacttc ggcaacgacg cgaacatcgg cggagtggtg cggaccgcca acgcgttcgc
    10981 cgtcgacacc gtgcacatcg tcggccgacg acgctggaac cggcgcggcg ccatggtgac
    11041 cgaccgctac cagcgcctgc gccaccacga caccaccgcc gaactgctga ccttcgccgc
    11101 ggccgccggg ttgaccgtcg tcgccgtcga caacatcccc ggtgcggtac gcatcgaaca
    11161 cacgccgttg ccgcgggcgt gcctgctgat cttcggccag gaggggccgg gcatcaccga
    11221 cgacgcgaag gccggcgccg ccgtcaccgt gtccatcgcg cagttcggat cgacccgaag
    11281 catcaacgcc gccgttgctg cgggcatcgc catgcacgcg tggatcactc aacatgggga
    11341 catttccaag gcctggtagg gcaggatcga cagcgtgact tcactgacgg gagatagcgc
    11401 cagcttcttt tcacgcagcg tgcgcgtgaa gggccagtcc agcccgcagg atgttgcgtg
    11461 cagcgtgttc gtcagcttgc gcagtgtgac cacatgcctg acaggcgaac acagcttggt
    11521 tgacgcggtt ccctcttgct gcgtaaccac agttctcgca gccatcggag gtgtgcttgg
    11581 ggtcgacctc gatccacctg cgtccagcgt cttccgcttt ggcgcgcaga atcgagacga
    11641 attgccccca cgcagcgtca ctaatgcttc ggttgagtcc ggtcttggcg gcggcccggt
    11701 tctgcaggaa cgctccgggt ttgtcgggat cagggagggg ttttgcacga cggcgcatgt
    11761 tggtgattgc cagatcctcc acaacaatca ggtcatagcg cgcaacgagt gcccgggcgg
    11821 ttttgtggtg gaagtcgcga cgttggttgg cgatcttgcg gtgccgagca gcggctgtct
    11881 ctcgccggtt ccgccggttg ttcgaggcac gcttcgcccg ggccaaccga acctgcgcgc
    11941 gagtcaaacg cgcggccgct cggcgctccc agcgggggtt actgatgtgt actccgtcgg
    12001 aggtggttgc gaaactagcg attcccaaat cgactccgac ctgccgccca gtgcctggca
    12061 gaggatcaac tgggacgtcg tcgcaagaca ggatcagcgc ccaccgccga ccctcgcggc
    12121 ggatttgaat tgtcttgacc aggccgacga cctgtcggtg cgcagtgacc ttcacctgcc
    12181 caatcccgcg cagatacacc cggttggtgt cgggatgcca acgggcgccg tcgccgtctt
    12241 tgggccactc cacactgtcg aaccgtccgc ggcccttaaa tcgcggatac cctggtgccg
    12301 caccgtcttt cacacgtcta aagaatccgt cgaaggcgcg attcaatcgg cgcaacgtgg
    12361 cctgctggct agaaaacgac catatcgcca cgtcgggacg atctcgccga atgtccttga
    12421 gctgaccgga ctgggtgcgg tagtcgatgg ccgtgcggct atgccgccac gcatccttgc
    12481 gttcctgcaa tgccgcgttg tacagctccc gatgcgattc caaacacgtc gccagtgcga
    12541 gatgctggcg taccgtcggt cgcatccgga acttgtaggt tcgcctcacg tcgagaccac
    12601 atcggatcgt gcgcggcacg cggttgactt cgacgcctgc aatatcgtcc cgtcaaagta
    12661 cggcttggac caagtgatgt tgtcaagagt caaatcaacc cactcgacac ggattcgcgc
    12721 gggcggggcg tcccgaaatc gccgccggtg cttggggcag tgggcaactc gttggcccag
    12781 tgccctcagt tcgcctgcac aacgacgaga gcgagtcacc agcatgctcc gaagactact
    12841 aagcacgacc gacaaatctc gcttgcatgc cctggtcacc agctttgacg tgcttgatga
    12901 ctctgcaagt ctcctcggaa ggtagcgatg gatcaggtat gggctaaccg cgcggcaagc
    12961 gcggaggccg cgatagccaa gcgacacctg aaacggctgt ggggattgcc tggaacccag
    13021 ctgggcgttg tcgcatggcc ggccgcgcgg aagtaccgga acttcgacac ctggcactac
    13081 tggtggcagg cgcacctatt ggacaacctg gtcgacgcgc acgtgcgcga cccacagccg
    13141 gaccggctga agcaaatcgc acgccagatc cgcggacatc ggttgcgcaa caacggatcc
    13201 tgggtcaacg actactacga cgacatggcg tggctggcgc tggcgttgga gcgggccggc
    13261 cggctggccg gagtcgagac acccggcgcg ctgaagaaac tgtccgagca gttcctcaat
    13321 gcgtgggtgc ccgaggacgg cggcggcatt ccgtggcgca agcaggacca gttcttcaac
    13381 gccccggcga acggaccagc ggccattttt ctcgcccgtt atgaagaccg gctgcggcgc
    13441 gcccagcaga tgtcggactg gatcgacgaa acgctgatcg acccggaaac ccatctggtg
    13501 ttcgacggca tcaaggccgg ctcgctggtg cgtgcccagt acacctactg ccagggcgtg
    13561 gtgatcgggc tggacgtcga gctggcgtcg cgcaccaagg accccgagca cgccgcgcgg
    13621 gtgcaccgtc tcgtcaaggc cgtgcgcgac aacatggcgc ccgagggcgt gatcaagggc
    13681 gcgggcggtg gcgacggcgg attgttcaac ggcgtcctgg cccgctacct cgcgctagtg
    13741 gtgacgacac tgccgcagaa cgggcctgcg gacgccgagg cccgcgacat tgcccgatcg
    13801 ctggtggtca agtccgcgga atcggcctgg gaaaaccggc aaaccgtcga cggcctgccg
    13861 ctgttcgccg cgttctggga ccgcaccgcc gaaatgccca ctgccggcgg gcaagaagcg
    13921 caattcgtcg agggcgcggt caacgcctcc gcagtgcccg aacgtgacct gtcggtgcag
    13981 ctgtcgggct ggatgctgat ggaagccgcc cacgcagtca ccgccgcgag cggcgacacc
    14041 ggcacggacg agtgaccgcc gagccacttc cgcggatcgt tcgcctgctg ccgggcaggt
    14101 tcagccgcga ccccaaggcg cccggcacga aagagaagtg tgcggcaggt ctgctgctcg
    14161 cgtgcaccgt ggcagcgatc ctgtgggcca attcgccctg ggcgcagacg tactgggcgc
    14221 tactggacac ccacgtcggc atcatgttcg cagggcacac cttcgaactg acggtgcagc
    14281 atctgatcaa cgacggcctg atgacgttct tcttcttcat cgtcggcctc gaagtcacca
    14341 gcgagttcgc catcggtggt ctcaccgacc gcgccagggc ggcggtaccc gtcgtcgcag
    14401 cgatcgcggg tctgaccgtc cccgccgtca tcttcctgtt gttcaacgcg tcgggcgaga
    14461 acgcgcgggc gtggggcgtg gtgatctcca ccgacaccgc gttcctcatc ggcgcgttgg
    14521 ccatcatcaa acccaaatac cctgcccggc tgaggatttt cctgctcacc ttggctgtgg
    14581 tcgacgacat cggcgcgctg tgcgtgatcg cgctcttcta ctccgagcgc atccaggtcg
    14641 ctccgctggt cgtcgcggtg gcattgatcg tcgcgatcgc attggtgcgg tttctgcccg
    14701 cggcccgcgg cccggcctac gccgtgctgg gctttgcgct gtgggtcgcg ctgtccgcgg
    14761 ccgggattca tcccaccctg gccggcgtcg cggtagcgct cctcatccct gtcttcacac
    14821 ccgaccgccg gcaggtcgag cagaccgtcg acatgatccg tgccttccgc cagtcaccca
    14881 actcccagta cgcccgcgcg gccactcgcg gtctgcggga gtcgatttcg atcaacgaac
    14941 ggctgcaggc agagatcggg ccctacgtgt cgtttgtggt gctgccgctg ttcgcgttgg
    15001 ccaacgcagg ggtccggctc gacatggaca gcgtgtcggc cgccatgcgg tcgcccctga
    15061 cgtggggcat cgtcgtaggc ctcgtggtcg gaaagttcat cggcatcacg ggcgcgacgt
    15121 ggatcatgca gcgcaccggt ctcgggcagc tggcgcctgg actcacattg cgccgggtcg
    15181 ccggcggcgc cgcgttgtcc ggaatcggtt tcaccatcgc acttttcatc gtggacatcg
    15241 cgatcagcga tccggggcgt caggatcagg cgcgcatcgg cgtgctcctc gcatccgtcg
    15301 tcgcgttcct ggcgggctgg gggatattcc ggatcacgga ccggatcagc ccgccggaac
    15361 cggtagggct caagctgatc cggccaatcg acccggaccg cgatcacatc cggggcgacc
    15421 cgaacgctcc gctgacactg gtggagtacg gcgactacga gtgcccgttc tgcagccgcg
    15481 ccaccggcgg gatcgacgag gtgctcgaac gcctcggcga cgatctgcgg tacgtgtggc
    15541 gccatcttcc tctggagcgg gtacacccac gcgcgttcga cgccgccagg gcggccgaag
    15601 cagcggggct gcagggcaag tacttcgaga tggggcggat gctgttcgca catcaggacg
    15661 atctggagtg gtccgacatc tatcgctacg ccaacgcgat cgggctcgac ctcgaacggt
    15721 tcgaccaaga cgtgcgtgtg catccgacga aggtgctgca gcgggtgcag gacgatgcgc
    15781 aggacgcgga gctgatggac ctcaactcga ccccgacgtt cttcgtcaac ggcaagcggc
    15841 acaagggccc gtgggatgcg gccagcctga tccgagcgct ggaagccggc aggatctagt
    15901 ggtgtgtctc gtcattggtt gactgcttgg agtgcgacgc aacgcttctc gttgaccgtc
    15961 cgacatgacc aagtcatccg ccggtgtcat cactcgcgag atttatcgca catctagcct
    16021 actatttggg agacacacga ctagcgtcct gctggagtag ggtgtggcga gcccggatcg
    16081 ggccggcgct taaaccatct ggtcttgttt cgccacgagc gggccgcagc gaccgcgaaa
    16141 atgatcccac cggcgaaggt cgacaaccac cccaccgcgc cacggtcgga ccacgaacac
    16201 cactgggtga cgatccgggc ggtgcaggca gcgatgccgc caacgacgag caccaggtac
    16261 acggtcgcgg ttggccggtg ccgcggaatt cgccgcagga cgagcagccc gcatagggtc
    16321 cacgcgagca gataaatcaa catggcgcag aacaggaacc agtagatttt cgaccaggcg
    16381 tcgccgggca cctcgtaaaa gttagaccgg tagaccttgg acccgttccc tgacgcccac
    16441 gcggcgaaca tcagcgggat gcaaacggtg gccggacgtt caacccactg cgtccctatt
    16501 gcttgtagtc ggtcgtcgtc gatgaccctg ctgagcacgg tttgcacgag agcgacggcg
    16561 gccagcaggt agctgacgtg cccgaggtag tcctcgacgt tcctcatgcc gctgatcgcg
    16621 tgcaagggag ggccgaggaa ttcggatgcc gtcggggata tcagtagcgc cgcgcaaccc
    16681 tggaaggcga tgcagagggt gcccgcgacc tcccaccggc taagccaggt gtgacgccgc
    16741 acccatagtg agcacgcgat gcaggcgagg gtgatcgaga tcagagcgac aaacatctga
    16801 gcgagaacga ctttcagcag gagggttaca aaccattaca ggggtggagc gtcttcgcga
    16861 tcacgccact catcacctcc cgcaacccgc cgcctgatct catgcagcag tgcgtcggtg
    16921 gagacctccc tgaggtcggt gattaacggg gtgtcgtcgt cctcgtagcc ccgcagggag
    16981 cgggccgcgg cgacgcgcac acgctccaca gagacgccga atccgttggc gattccctcc
    17041 aaggtggatt cgtccggcat ctgccccagt gtcgggcggt cgtcgtgcag gatcttccaa
    17101 acgagctgcc ggcttagccc ggagcggtgc acaaggtcgg cgggtgtcca gccgcgagca
    17161 tcgagctcgg cttggacgaa ccttcggtac tcgtgcacga aaacgagcct tccgtcgctt
    17221 gcgttcggtg tccacggcac tgatcgagtg ccgtcaatcc gtaatggtct acgactacgc
    17281 gacttcgtgt caatcccgtc gcctattctg acctgcgact tggcgtacca agtcgaggta
    17341 acttgttgac gcttcgtcaa cgagtatgaa acattgtgtt ctgaagaggt tgacaaccaa
    17401 catccaacca ggctgctgtc cgacccggat ggcaatccga gcatctctcc gaaggatcgg
    17461 gcgaatgagc aacaaagctg taagggccaa gaagcgccgt gctgctagag caaagcgggt
    17521 cgccatgatg ggggccgtct cagcgactgc tgtggccgtg gggctaacgc cggcgacggc
    17581 caatgcggca tcgagccaga cctacttcat cgggttccct gactggctcc cgatcggtga
    17641 aggcgcgacg cttccgacgg acccagtcgc catcaacaat gcgatggtcg gagccaagga
    17701 cagcaacccc ctcattggtt gggggaccgg cggcgtcaac ctgcaaccgg tatgggtcag
    17761 gtgggtcgac ggggtgccgc aatacacgct gccagtcgtg ggccagacgg gatcgcatcc
    17821 ggagacgaat cctgcctacc aacccgcata cgatgcggct tattgggccg catataactt
    17881 atggtcctgg ctcggcggct gccagggcag atcgaatcgt gctcaatgtg cgacgaatgc
    17941 cgcattgagc gcggtctcgg gaataccgca gacgatcacc gtgcccgact acggagtcct
    18001 cgaagacggc tactggacca ctaccacggc aggccagtgg gtgacgtcgt ccgacgtagg
    18061 tgatctgccg accgcggctc aactcgccta cgccgcttac gttctgcaga atggcgatct
    18121 gagtgccctt gcgccattgt tgaattggac ggcgtacttc accaacgtca atctcatagc
    18181 atacggcgac ggcgccatcg ccgccgggct ggcctaccag gcgttcatcg acagcgcaca
    18241 gggcgagacc cacgagggct acgatccgta cgccgtaggt gcggcgctga ccggaccgcg
    18301 aaagatcgtc atcatcgatc ctaccggcaa ggtgacactg atcagcgtcg acgagacaaa
    18361 taacccactc gacctccccg agatcatcta tcccggttcg ggagagatgc cggactatga
    18421 gtcggcccag gacggtggcg tcctcgacct gacggcgctt tcgcttgtgc tggtgcgtaa
    18481 cccgggccgc gcgaacggcg ggttgtatgc ccggttcgcg ccgatctacc aggagttgac
    18541 cggcgtcaac cccgtgacgc ccgagcgtca ggacgtgctg ccggcgggtg tcgacccagc
    18601 gctgatcacg aaattgctca ccggcaacgc cggtgacatc agcctcaatg acgtcggcga
    18661 tctgcaagct gtgatcgaat cggccgacgg caagccgatc atcgtcacgc tgaaagccga
    18721 tatcggctgg gagtacgacc tgttgtcgga tgccccggcg acagcgaacc cgatcgcgtg
    18781 ggccaactcg gtggcgtcgt ccatcttcct gacgaacctg ttgaccgggg tcgacttcca
    18841 agatctcggc gacggcgcgt ccatcggccc ggatggcacg ctctactaca cccttccggt
    18901 cgatgagctg ccgctgctgg cgccgatgcg gttgccggcg caggcaatcg gcttggtcac
    18961 aggcaatccc gacgtcaaca caccgcttgc ggatgcgatc gagccggcgc tgaagatgct
    19021 cgtcaacctc ggctacaccg atgtcgtccg gaaccccgat ggcacctaca cgcggacgct
    19081 ggacaggttc ggcgaaccca cgctgttcgg aacacccacg ctgactcgtg agcagatggc
    19141 actggtgcct ggtgatctga tcgcagctct tggtcggggc ttcggggatg aaatgaccga
    19201 tgttctgctg cggacgaagg gtcagctcac agaagcgctc aaagtcgaga tgacagacga
    19261 acaggatgag gccgtcgagc aggcactctc ggctcccggc accgccatca agaacgccag
    19321 tcacgatgtg ggcaacggtg tctcgcaggt actgacagct gtcgagtcgg agctgccgga
    19381 ggcgcccgcg gtcacccagg acgatttgga ggagggccag cggaaggttg gtcggcagct
    19441 cgcggacaca cgtgatcaga tcaacgaaac cgtcaccaag gtgcagtcgg tgatcggtaa
    19501 cgggcgcaac accgccacga ccgccaacac cagtgatggc ggcgagaacg gggacacacc
    19561 gagcaccacg cggaagacgc cggtcaagga tgcgcttcag aaggccaaca gtgacatcaa
    19621 gaagaccgtc actagtgtcc gcgaccaggt caagaaagcc tttggcggcg aaaaggattc
    19681 ggacggtagc ggcaatggcg atggggggga aggcggagcg cagtaaatcc gccaagtcga
    19741 aacagccccg gggtcgaggg gcttcggggc tgtttcacgt atgccgccac tgctagctcc
    19801 acagcgtgac gtggattttg ggtcgaaatc gcgtgacgcc gacgcccgtg ccacgcagca
    19861 tggcctgggt gcaccgcggc gtcgtgatga accggacaag ttcggagacc gcgggctggc
    19921 gggcggccgg ggtgagcgtg aatgcgcacc attcgcccgg cacctgcaac ccctgcccct
    19981 tgacgtgaat cagccggcca gctgccaggt ccttcccgac cgtgaagccg atcgccaggc
    20041 tgaccccgcc gacgcgctgg acctcttcca gcgccgcggc atcgctttgg aaaatccgtt
    20101 ggtgggattc cggaatcgcc aagttccgca ccacggccgc gatctcaccg tcgacactgc
    20161 cggctgacgg ccccagcatc catagctgtt cgcgcagcaa cgcgggtgtc ggaggatggg
    20221 tgatcagcgg actccccggt gccgcgacgg tgaggatctg atagttcaga aatggccgga
    20281 cgacgatcgc gttgtcggtg gcctggcggt cgtcactggc ctgaccgagc gcaatgtcga
    20341 cggcgcgtga ggagatcaac tccttgaacc tgctcgtcgg gtgcacgctc aattcgaccg
    20401 agagatcgtt ggcgcgtgac gagaacagtt cgatcagccc tggcgcggca tgctcggcga
    20461 atgcgctgga cgccgcgatg cgcagcaggc gccgcccgtg cgcggcctcg gtcacctcga
    20521 tcgcagtctg ttgctgcagg ccgaggatct cgacggcgcg gctggccagg cgcagccctc
    20581 ctggcgtgaa tgccagtccc gccgaggtcc gggagaacaa ctggtcatcc agttccttgc
    20641 gcagctgagc tacgtgcatg gagatcccgg cgtcggacat gccaagttcc tcggccgccc
    20701 catggaccga acccaggcgg accacggcgg aaaaggcccg aagctgagcc ggtgtcacgg
    20761 atcgagccta ctgactgact taagtgattg cttaacccgt tattgacggc cccgacgtgc
    20821 gggcgaacac tggcgtcctc agcacacgag tgtgaggtca gtcatatgca ggttcctgga
    20881 cctttcgagt acgaacgcgc cacaagcgtc gatcacgcca tcgggctgct cgaccggctc
    20941 ggtgacggag cgatgctcgt cgcaggcggc cacagcttgt tgccgatgat gaaacttcgc
    21001 atcgccaacc ccgagtacct ggtcgacatc aacgacctga ttcccgagct cggatatatc
    21061 gtcgtcgatc cgacgctcgt gcgtattggt gcgatgacgc gtcaccgcga agcactggag
    21121 tcggaggacc tggcggccgt ctgccccatc ttccgcgacg ccgaacgcgt catcgccgac
    21181 cccgtcgtgc gcaaccgcgg cactgtaggg ggatcgcttt gtcaggccga cccggccgag
    21241 gatctcacga ccgtgtgcac ggtgctgaac gcggtgtgcc tcgcccgggg accctccgga
    21301 gagcgcgaaa tctcgatcga cgattttctg accgggccgt atgagacatc actggcccac
    21361 aacgagatgc tcatcgagat acgaattccg ttgcggcaca atgcctcaag cgcatacgcc
    21421 aaggtggagc ggcgagtcgg cgactgggcg gtggcggcag ccggcgccgc ggtgagactc
    21481 gacggcgacg ccattgttgc ggcgaggctg ggtctcacgg cggtcaacac cgacgctgcc
    21541 gcgctggcag cggtgggtgg gggccttgcc gggcggcccg ccaccgaaga gactttcgcg
    21601 caggccggcc gggacgccgc acaggcctgc agcccggtaa ccgacatgcg gggcagcgcg
    21661 gaatacaagc gccacctcgc ctccgaactc accgtccgaa cgttgcgtac tgcggtcgag
    21721 cgcgtacgca gcgccacggc tgtggaaggg aactagcgat gcaagtgaac atgaccgtca
    21781 acggcgagcc ggtcaccgcc gaggtcgagc cccggacact gcttgtgcat tttctgcggg
    21841 atcagttgcg gctcaccggg acacattggg gctgcgacac gagtaactgc ggcacctgcg
    21901 tcgtcgaaat ggatggcgaa ccagtgaagt cctgcaccgt gctcgcagcg atggccgctg
    21961 gccgcagcat cctcacggtg gagggccttg aggtcgacgg ccacctcgac cctgtgcagg
    22021 aaggcttcat gcgctgccac gggctgcagt gcggattctg tac
//
LOCUS       DASRJA010000207         7372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1837846,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000207 DASRJA010000000
VERSION     DASRJA010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837846"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFC01_09265"
     CDS             <1..215
                     /locus_tag="VFC01_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HZN79860.1"
                     /translation="AGGIDDHVLGGWPAVMIGYPAAVSVPLAFATMILVSRLTRAAVP
                     ADVAQNFARMHVPERLGMGIERVPRG"
     gene            360..710
                     /locus_tag="VFC01_09270"
     CDS             360..710
                     /locus_tag="VFC01_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HZN79861.1"
                     /translation="MSQTDLPHGETPVSGHDYLAVQASPEFQDLRSRLRRFVFPMSAL
                     FLVWYFAYVLLGAFAHDFMAIKVWGNINIGLLIGLGQFVSTFVITGIYVWWANRELDP
                     RAEAIRAKLEGGAS"
     gene            707..2347
                     /locus_tag="VFC01_09275"
     CDS             707..2347
                     /locus_tag="VFC01_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HZN79862.1"
                     /translation="MTIKILAADGATVGNPVANIGIFALFVAITLYIVIRASKQNTTA
                     AEFFTAGRAFTGPQNGIAISGDYLSAASFLGIAGAIAVYGYDGFLYSIGFLVAWLVAL
                     LLVAELLRNAGKFTMADVLSFRLKQRPVRIAAATTTLAVCLFYLLAQMAGAGGLVALL
                     LNVKGTLGQALVIAVVGLLMILYVLIGGMKGTTWVQIIKAALLIGGAALMTVMVLAKF
                     GLNFSEILGAAQSEVSGSATEAVAKRDVLAPGAQYGASLTSRINFISLALALVLGTAG
                     LPHVLMRFYTVPTAKEARRSVVWAIALIGAFYLFTLALGYGAAALVGPDEILAAPGAQ
                     NSAAPLLAFHLGGVVLLGVISAVAFATILAVVAGLTITAATSFAHDIYASVMKSHQVT
                     EDEQVRVSRITVVVIGILAIGLGILAAEQNIAFLVALAFAVAAAANLPTILYSLYWRR
                     FNTRGALWSMYGGLISTIVLIVFSPAVSGAKTAMLPNVDFAWFPLANPGIVSIPLAFL
                     LGILGTLTSPDDEDPEIAAEFEVRSLTGVGAEKAVAVH"
     gene            2442..3644
                     /locus_tag="VFC01_09280"
     CDS             2442..3644
                     /locus_tag="VFC01_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN79863.1"
                     /translation="MTARRFAFGLLAYAFAAIMVGTTLPTPMYALYGQQMHFAVLTTT
                     VIYATYAGGVLFALLAFGRWSDAIGRRPILLAGVVFALASAAVFLVADSVPLLLVGRV
                     LSGLSAGVFTGTATAAVIEAAPDRWRSKAAAVATVANIGGLGTGPLLAGLLVEYAPAP
                     LHLSFIVHIGLALLAGLAVLVVPETSQRTGRIGVQRLSVPTEVRAVFVVAALAAFAGF
                     AVTGLFAAVAPSFLSQVVGIDNHAVAGLIAGSIFFASAVAQIAASNLNPQRAVAIGCA
                     ILVAGMVILAVALKLSSFVGLIAAAVVAGIGQGISFSRGLAAVADRTPADRRAEVSST
                     YFVVAYVAISLPVVGEGIAARQWGLQTAGETFAIAVAILSAICLVGILVLEARQPRNA
                     ELATTRTG"
     gene            complement(3625..4788)
                     /locus_tag="VFC01_09285"
     CDS             complement(3625..4788)
                     /locus_tag="VFC01_09285"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZN79864.1"
                     /translation="MMAALAVDCPLRADALHAKAIADTGLDDFGPDDYRERLDVYLAA
                     LYDIDDMHAPGIVNFFGQLSQWLKNRLLLTDLLKRHPEINDIDLLAPVVIAGLPRTGT
                     THLHNLLAAAPTFRTLPYWESFEPFPLPTEVGIEPDPRAARMDVAVQVMEIVMPHFAL
                     MHEMTTEHVHEEIQLLANDVSTMFMETLAHVPRWTEYYLAHDQTSTYEYLATQLKALQ
                     FLRGGRRWLLKSPQHLEQLPVLERVFPGVAVVCTHRDPVPVAISMVAMLTYSARMHRS
                     FVPVHEIAAAWIDRLELMLAALVRDRDVVAPERSVDVRFDDFMADELGVAEQVYGLVG
                     EPVTDDARDAITGYLAGHQRGRLGKVATSCEMFGLDEDELRTRFAPYVNRFLS"
     gene            4901..5476
                     /locus_tag="VFC01_09290"
     CDS             4901..5476
                     /locus_tag="VFC01_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN79865.1"
                     /translation="MTTEIGRPRDPRIDGAVLQATVDLLGETGYAELSVDAIARRAGT
                     SKPAIYRRWPSKAHLVHEAVFPLSEATELPATGSLADDVHEMVRRTVTVLTTPAARAA
                     LPGLVGEMAADLTLHAALLERFGDILGRGLTERLDKALARKEVRPDVTAAELSEALAG
                     LTFLALLTRSAALDDAWVERTATLILRGISS"
     gene            5473..6576
                     /locus_tag="VFC01_09295"
     CDS             5473..6576
                     /locus_tag="VFC01_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HZN79866.1"
                     /translation="MTHESTAAWRELLETLGGLDHSFLDGDRAVTDDRHIADGYRMLA
                     TTLGVALDTYLFAEPSRPQFVEVNTPFRRDRRWGGDNTDSYYRLCPVDPKRRYRISGN
                     KGDSVYFSVTAYNEPSPGAWSDRIVAIVRDDDLDIDDAGNFTFELGPTADGVVLMTRD
                     YQADPLTGRPVSWHIEALDEPEPIRHGDAETAAALRASAAWLRTMFAIVPLAVGGRVD
                     DSHQLGHEISQVANQFADPYQVPDANFGWSARDACYAYGSFVLEDDEALVITHRPPTC
                     RFWNLVVWNQFMATHNVNDARSSINGHSAVPNADGTVTIVVSRGMTAHPNSLTTLDYP
                     RGNLAFRWFLADDVPAKPEVQLVKVSDAPTSVD"
     gene            6576..7046
                     /locus_tag="VFC01_09300"
     CDS             6576..7046
                     /locus_tag="VFC01_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN79867.1"
                     /translation="MSELTDLRFCHLVVGVTDMDRALSFYRGLLGMDVVFDRLISGES
                     FDAALHASRKQEGRVVGGVIGGLMIELLSLGGSKPAEAPKRCGVVGIQNISLSVTDLD
                     DTYRRITEAGYEPDQKPFEIAGVRMFFVKDPDGTPVEFIELPGGARSTYEMYRA"
     gene            7043..>7372
                     /locus_tag="VFC01_09305"
     CDS             7043..>7372
                     /locus_tag="VFC01_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN79868.1"
                     /translation="MTYSHPESLRGHAAIVTGAARGIGKGIATALVERGASVLLVDIS
                     DEHLAATTEELSAAGHSAVHLLADLRDPDAPARIVTKAVERFGAIHGLVNNAMATREP
                     KPFADITG"
BASE COUNT         1160 a         2473 c         2373 g         1366 t
ORIGIN      
        1 tcgcgggcgg catcgacgac cacgtcctcg gcggctggcc cgcggtgatg atcggctatc
       61 cggcggcggt gagtgttccg ctggcgttcg cgacgatgat cctggtcagc cgcctcaccc
      121 gggccgccgt gcctgccgac gtggcacaga acttcgcccg catgcatgtg ccggagcggc
      181 tcggcatggg catcgagcgt gtcccgcgcg gctgagcccg caaccgctcg tcgccccgca
      241 tcgaccgttc accgcaccgc ctcccggttt caccgtgtta acgctgctag cagtgggtat
      301 gtgactcaca tctcaattag gttttcgccg cagagccggt tcacattcga ggagtcctgg
      361 tgtcgcaaac agacctaccg cacggggaaa cccccgtcag cggtcacgat tatctggccg
      421 tacaggccag tcccgagttt caggacctac gaagcaggct gcgccgcttc gtctttccga
      481 tgagcgcact gttcctggtc tggtatttcg cctatgtgtt gctcggcgca ttcgcgcacg
      541 acttcatggc catcaaggtg tggggcaaca tcaacatcgg cctgctcatc ggccttggcc
      601 agttcgtgtc gacattcgtc atcaccggca tctacgtctg gtgggcaaac cgtgaactcg
      661 acccgcgtgc cgaagcgatt cgcgccaaac ttgaaggcgg cgcgtcatga ccatcaaaat
      721 cctcgccgcc gacggcgcca ccgtcggcaa tcccgtcgcc aacatcggca tcttcgcgct
      781 cttcgttgcg atcacgcttt acatcgtgat tcgcgcgagc aagcagaaca ccaccgccgc
      841 ggagttcttc accgcggggc gcgcgttcac cggaccgcag aacggcatcg cgatctccgg
      901 cgactacctg tcggccgcca gctttctcgg catcgccggc gccatcgcgg tgtacggcta
      961 cgacggcttc ctgtactcca tcggcttcct ggtcgcctgg ctcgtcgctt tactattggt
     1021 tgccgaatta ttgcgcaacg caggcaaatt cacgatggcc gacgtgctga gcttccggct
     1081 gaagcaacgg ccggtgcgga ttgccgcggc caccaccacc cttgccgtat gccttttcta
     1141 tctgctggcc cagatggcgg gcgccggcgg tttggtcgca ctgctgctca acgtcaaagg
     1201 cactctcggc caagccctcg tcatcgcggt cgtcggcttg ctaatgatcc tctacgtgct
     1261 gatcggtgga atgaagggca ccacctgggt gcagatcatc aaggccgccc tgttgatcgg
     1321 cggcgccgca ctaatgacgg tgatggtgct ggccaagttc ggcctgaact tctccgagat
     1381 cctcggcgcc gcccagtcgg aggtctcggg atccgccacc gaagccgtcg ccaagcgcga
     1441 cgtgctggcc cccggcgcgc agtacggcgc ctcactgacg tcgcggatca acttcatttc
     1501 cctggcgctg gcactggtgc tcggcacggc aggcctgccg cacgtgctga tgcgcttcta
     1561 caccgtgccg accgccaagg aagcccgccg gtcggtggtg tgggcgatcg ccctcatcgg
     1621 cgccttctac ctgttcacgc tggcgctggg ctacggtgcc gcagccctcg tcggtcccga
     1681 cgaaatcctg gcggctcccg gcgcccagaa ctccgcggca ccgctgctgg cgttccacct
     1741 cggcggcgtg gtcctgcttg gcgtcatctc ggcggttgcg tttgcgacga tcctcgccgt
     1801 ggtcgccggc ctaaccatca ccgcggcaac gtcgttcgcg cacgacatct atgccagcgt
     1861 gatgaagagc caccaggtga ccgaggacga gcaggtgcgc gtgtcacgca tcacggtggt
     1921 ggtgatcggc atcctcgcga tcggccttgg catcctggcc gccgagcaga acatcgcgtt
     1981 cctggtcgcg ctggcgttcg cggtcgccgc ggcggccaac ctgcccacga tcctgtactc
     2041 gctgtactgg cggcgattca acacccgcgg cgcactgtgg agcatgtacg gcggcctgat
     2101 ctcgacgatc gtgctgatcg tcttctcccc cgcggtgtcg ggtgctaaga ccgcgatgct
     2161 gcccaacgtg gacttcgcct ggttcccact cgcgaatccg ggcatcgtgt ccatcccgct
     2221 ggcgttcctg ctgggcatcc tcggcacgct gacctcgccg gacgacgaag atccggagat
     2281 tgccgccgaa ttcgaggtgc gatcccttac gggcgtcggc gccgagaagg cagttgccgt
     2341 ccactgatct ccgccgctca ggtggcgccg ccggaacccc ccgccggcgg cgccaccgct
     2401 tatcgggatt accgaggccg ccgggtgggt tatcttcacc cgtgaccgcc agacgattcg
     2461 ccttcggcct gctggcctac gccttcgcgg ccatcatggt cggcaccacg ttgccgacgc
     2521 cgatgtatgc gctgtacggc cagcagatgc actttgcagt gctgacgacc accgtgatct
     2581 atgcgacgta tgcgggtgga gtgctgttcg cgctgctggc gttcgggcgg tggtccgatg
     2641 cgatcggccg acggcccatc ttgttggccg gtgtggtgtt cgcgttggcg agcgcggcgg
     2701 tgttcctggt cgccgactcg gtgcctctgc ttctggtcgg ccgggtgtta tcggggttgt
     2761 cggcgggcgt gttcaccggc accgccaccg cggcggtcat tgaggcggcg cccgataggt
     2821 ggcgaagcaa ggcagcggcg gtggcgaccg tcgccaacat cggggggctg ggcaccggac
     2881 cgctgctggc cggcctgctg gtcgaatacg cacctgcgcc actgcatctc agcttcatcg
     2941 tgcacatcgg gctggcgctg ctggccgggc tggcggtgct ggtcgtgccg gaaacctcac
     3001 agcgcactgg ccggatcggc gtgcaacgtc tgtcggtgcc caccgaggtt cgcgcagttt
     3061 tcgtcgtcgc ggccctggcc gcgttcgcag gcttcgcggt gacgggcctc tttgccgcgg
     3121 tcgcgccgtc gtttctctcc caggtcgtcg ggatcgacaa ccacgcggtt gccggtctga
     3181 tcgccggctc gatcttcttc gcgtcggcgg tggcacagat cgcagccagc aacctcaacc
     3241 cgcagcgtgc tgttgcgatc ggctgcgcga tcctggtagc tggcatggtg attctcgctg
     3301 tggcactgaa gctttcgtcg tttgtcgggc ttatcgcggc cgcggtggtg gcgggcatcg
     3361 ggcagggcat cagcttcagc cgcgggttgg cagcggtggc cgaccgcacg cctgccgatc
     3421 gtcgcgccga ggtcagctcc acctacttcg tcgtcgccta cgtggcgatc tcattgcccg
     3481 tggtcggcga gggcatagcg gctcggcagt ggggattgca aacggccggt gagactttcg
     3541 cgatcgccgt tgcgatcctg tcggccattt gcctggttgg cattcttgtg ctggaggcgc
     3601 gccagccccg caacgcggag ctggctacga caagaaccgg ttgacgtacg gcgcgaaacg
     3661 cgtccgcagc tcgtcttcgt cgagtccgaa catttcgcac gacgtagcca ccttgccgag
     3721 tcggccccgc tggtggccgg ccagatagcc ggttatcgcg tcgcgtgcgt cgtcggtcac
     3781 cggttctccg acgaggccgt aaacctgttc ggcgacaccg agttcgtcgg ccatgaagtc
     3841 gtcgaaacgc acgtcgaccg agcgctcggg agcgacgaca tcgcgatcgc gaaccagcgc
     3901 tgcgagcatc agctcgagcc gatcgatcca cgcggcggcg atctcgtgta ccggcacgaa
     3961 cgaccggtgc atgcgcgcgg agtaggtgag catcgcgacc atggaaatcg ccaccggcac
     4021 cgggtcacga tgcgtgcaca cgacggccac gcccgggaag acccgctcga gcacggggag
     4081 ctgctcgaga tgctgcggag atttcagcag ccaccgcctg ccgccccgca ggaactgcag
     4141 cgccttgagc tgcgtcgcca ggtactcgta ggtcgacgtc tggtcgtgcg ccaggtagta
     4201 ctccgtccac cgcggcacat gcgcgagagt ctccatgaac atcgtcgaca catcgttggc
     4261 cagcagctgg atctcctcgt ggacatgctc ggtggtcatc tcgtgcatga gcgcgaaatg
     4321 cggcatgacg atctccatga cctggactgc gacgtccatg cgcgccgccc ggggatccgg
     4381 ttcaatcccc acttcggtcg gcagcgggaa cggttcgaag ctctcccagt acggcagcgt
     4441 gcggaacgtc ggcgccgcgg caagcagatt gtgcaggtgc gtggtgccgg tgcgcggcag
     4501 tcccgcgatc accaccggcg ccagcagatc gatgtcgttg atctcgggat ggcgcttcag
     4561 caggtcggtc agcagcagcc ggttcttgag ccattgcgac agctggccga agaagttcac
     4621 gatgccgggg gcgtgcatgt cgtcgatgtc gtacagcgcc gcgaggtaga cgtcgagcct
     4681 ctcccgatag tcgtcgggcc cgaagtcgtc gaggccggtg tcggcaatcg ctttggcgtg
     4741 caacgcatcc gcgcggagcg ggcagtcgac cgccagcgcg gccatcatgt cgcggatctg
     4801 ctgggcctcg gggctgaacc ggggctcggc gaggtcgtcg agctggacga attctggtgt
     4861 ggcgtgggtc acggcgactt atgttactct gagtttcgta atgacgacag agattggccg
     4921 acctcgcgat ccgcgcattg atggtgcggt gcttcaggcg acggtcgacc tgctcggtga
     4981 gaccggatat gccgagctct cggtcgatgc gatcgcccgg cgagccggta cgagcaagcc
     5041 cgcgatctac cggcgctggc cgagcaaggc gcacctcgta catgaggcgg ttttcccgct
     5101 cagcgaagcc accgagctac ccgccaccgg atcattggcc gacgatgtgc acgagatggt
     5161 gcgccgcacg gtgacggtat tgaccacccc agctgcgcga gcggcgctgc caggactcgt
     5221 cggcgagatg gctgcggacc tgaccctgca cgctgcactc ctcgagcggt tcggcgacat
     5281 cctggggcgc ggtctgaccg agcggctgga caaggccttg gcgcgcaagg aggttcgacc
     5341 cgacgtcacc gcggctgaac tgtccgaggc gctcgccggc ctcactttct tggcgctcct
     5401 cacccgaagc gcggcactcg acgacgcctg ggtcgagcgc actgccaccc tgatattgag
     5461 aggaatcagc tcatgactca cgaatccaca gcggcgtggc gcgagctgct cgaaaccctt
     5521 ggcgggcttg accattcctt cctcgacggt gatcgtgcgg tcaccgacga ccggcacatc
     5581 gccgatggtt accgcatgct cgcaacgacg ctgggcgtag cgctcgatac ctacctgttc
     5641 gcggagccga gccgacctca gttcgtcgaa gtcaacacgc cgttccgacg ggaccggcgc
     5701 tggggcggcg acaacaccga ctcctactac cgcttgtgtc cggtggatcc gaagcgccgc
     5761 taccgcatca gcggcaacaa gggcgacagc gtgtacttct cggtgaccgc ctacaacgaa
     5821 ccatcgcccg gcgcttggtc ggaccggatc gtggcgatcg tgcgcgacga cgatctcgat
     5881 atcgacgacg ccggcaactt cacgttcgag ctggggccga cggccgatgg cgtggtgctg
     5941 atgacccgcg actaccaggc cgatccgctc accggcaggc ccgtcagctg gcacatcgag
     6001 gcgcttgacg agcccgagcc catccggcac ggcgacgctg aaacggctgc ggcgcttcgg
     6061 gccagcgccg cctggctgcg caccatgttc gcgatcgtcc cgcttgcggt gggggggcgc
     6121 gtcgacgact cccaccagct cggtcacgag atatcgcaag tggcaaacca attcgccgat
     6181 ccttaccagg tgccggacgc caacttcggc tggtctgccc gcgacgcgtg ctacgcctac
     6241 ggcagcttcg tcctcgagga cgacgaggcg ctggtgatca cccatcggcc gccgacgtgc
     6301 cggttctgga acctggtggt gtggaaccag ttcatggcca cccacaacgt caacgacgcg
     6361 cgcagctcga tcaacggcca cagcgcggtc ccgaacgcgg acggtactgt cacgatcgtc
     6421 gtctcccgcg gcatgactgc gcacccgaat tccctgacca cactggacta tccgcgtggc
     6481 aacctcgcct tcaggtggtt ccttgccgac gacgtcccag cgaaaccgga ggtgcagttg
     6541 gtgaaggtgt ccgacgcgcc gacgagcgtc gactagtgag tgagttgacc gatttgcgat
     6601 tctgccactt ggtcgtcggc gtcaccgaca tggatcgcgc cctgtccttc taccgcggcc
     6661 tgctcggcat ggacgtcgtg ttcgaccggc tgatctcagg cgaatcgttc gacgcggcgc
     6721 tgcacgcgtc ccgcaagcaa gagggccggg tcgtcggcgg cgtgatcggt gggctgatga
     6781 tcgagctgct gtccctcggc ggatccaaac cggccgaagc gcccaagcgt tgcggcgtcg
     6841 tcggcatcca gaacatctcg ctgtccgtca ccgatctcga cgacacttac cgccgaatca
     6901 ccgaggccgg atacgagccg gatcagaagc ctttcgaaat cgccggcgta cgaatgttct
     6961 tcgtcaagga tcccgacggt accccagtcg agttcatcga gctgcccggc ggcgcgcgca
     7021 gcacgtacga aatgtaccga gcgtgaccta ttcgcacccc gagtcgcttc gcggacacgc
     7081 ggccattgtc accggtgctg ctcgcggcat cggtaagggc attgcgacag cactggtgga
     7141 gcgaggcgcc tccgtcctcc tcgtggacat ctcggacgag cacctcgccg ccaccaccga
     7201 agagctcagc gccgcaggcc attccgctgt acacctgtta gcagacctgc gcgatccaga
     7261 tgcccctgca cgcattgtca ctaaggccgt cgagcgcttc ggtgcgatcc atggcttggt
     7321 caataacgcc atggcgacca gagaacccaa acccttcgcc gacatcaccg gc
//
LOCUS       DASRJA010000208         7046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2685822,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000208 DASRJA010000000
VERSION     DASRJA010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2685822"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFC01_09310"
     CDS             complement(<1..230)
                     /locus_tag="VFC01_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN79869.1"
                     /translation="MDYGLVLFTSDRGITPAAAAKLADDHGFATFYVPEHTHIPVKRQ
                     AAHPTTGDESLPDDRYMRTLDPWVSLGTAAAVT"
     gene            396..923
                     /locus_tag="VFC01_09315"
     CDS             396..923
                     /locus_tag="VFC01_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79870.1"
                     /translation="MNSGATTPTKLREWVRRYLPCEIAGTVGELGGAAVAYLATGSLA
                     AAAITATIGASAGYYAAAYVNALRWSYRDHAYRRWPSRLLMSNLLALRSVAVEFGPAE
                     LIDSIAVRPVAFYVGPLIFDNTIVGWIFGKLISDLAFYVCAIASYERFTGLLVGARPR
                     AEEVTDASVPTIEAA"
     gene            892..2049
                     /locus_tag="VFC01_09320"
     CDS             892..2049
                     /locus_tag="VFC01_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HZN79871.1"
                     /translation="MHPSRRSKLREDLRADTQRLTALAERHGTPLLVLQPHLVARRYR
                     ELTAKLPGFGMHYAVKASPHPLVLSTIAICGGGFDVASSAEVDLLQQLGLPMDRCIHT
                     HPIKKPADIDHAYAAGIRTFVVENPCEAQKFAGRPDDIELLVRLAFRNPAAKSDLSSK
                     FGVEPAEAELLVKHVIAAGVQFAGFSFHVGSQGSSAQPFDTALRGTLDLVTDIAASLG
                     VHTRVIDIGGGFPVAYRESMPTLDEIAGVIDGALGPARDDFTLLAEPGRYLVADSMTL
                     LTSVVGTAQRDGQTWHYLDDGIYGSYSNVMTEDVHPPILALRDLANGAGEFEPVTLAG
                     PTCDSADVIARDYPMPALEVGDVVISPMMGAYTSVTSSRFNGIAATPIVMS"
     gene            2108..3238
                     /gene="sfnG"
                     /locus_tag="VFC01_09325"
     CDS             2108..3238
                     /gene="sfnG"
                     /locus_tag="VFC01_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfone monooxygenase SfnG"
                     /protein_id="HZN79872.1"
                     /translation="MTTERIADHVKFAYWVPNVSGGLVTSDIEQRTDWNYDYNAKLAQ
                     TAENNGFEYALSQVRYEASYGAEYQHESTSFSLALLMATQKLKVIAAVHPGLWQPGVL
                     AKLGATADHLSGGRFAVNVVSGWFKDEFTHLGEPWLEHDERYRRSAEFLQVLRKIWTE
                     DNVDFRGDFYRIHDFTLKPKPLNTPERPNPEIFQGGNSTAARRNGGYYADWYFSNGKD
                     FDGLTEQVVEVRDHAREVGREVRFGLNGFIIARDTEKEAREVLREIIDKANRPAVEGF
                     RSAVQQAGNSTLDKRGMWADSTFEDLVQYNDGFRTQLIGTPEQIAERVAAYRKRGVDL
                     ILGGFLHFQEEIEYFGAKVLPLVREIEDSEEDSVDSPVLVSA"
     gene            3317..4168
                     /locus_tag="VFC01_09330"
     CDS             3317..4168
                     /locus_tag="VFC01_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5336 domain-containing protein"
                     /protein_id="HZN79873.1"
                     /translation="MSYPPGSPGYPPAQQPTTQFSAPTQQFGKLPEPAAPAAAEGPSK
                     LPVYLTAAVAALGLAVYLSNYGPLFEAAGGEFFTPTLLDLGVVASLLAALIAGVGLIP
                     KQKTSPALVAVLSVLGFLLVVSIVLTAPQGVTIEWGLYLIIAFSVVQAIVAVAVLLFD
                     AGVIAPSVPKPRYEPQQYGQYGGQGPYYGQPHQPGPPPQQQQLRPGYPSPYPGGYPGG
                     PPSGGYSGGQQSGPPTPPTGFPTYGQPPSSSNAGNAGNAGNSSSAPTTQVPVQPSSSA
                     QSSPPPS"
     gene            4229..5545
                     /locus_tag="VFC01_09335"
     CDS             4229..5545
                     /locus_tag="VFC01_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HZN79874.1"
                     /translation="MENRPVGTRNARELLRVAFGPSVVALVVIAAVVLLQLLIANSDM
                     TGALGATASMWLGVHQVPVSIGGRELGVMPLLPVLAMVWGTARTTASATSPQASWFVT
                     RWVVASALGGPLLIAAISLAVIHDAASVLTELQTPNALRAFAGVLGVHAVGAVIGVGS
                     RVGRRTLTASPLPKWLPDAFRAATAGVLALFGLSGVVMVGSLVVHWSTMHDLYAITDS
                     VFGQFSLTVLSVLYAPNVMVGTAAVAVGSSTHVGLATFSAFTVFGGDIPALPVLAAVP
                     SPPLGPVWVALLIVAAASGVAVGQQCARRPLPLLPALGKLVVASACAALAMALLGFAG
                     GGRLGNFGDVGVDQVTFAPAVFLWFVGIGALTVAMSGGIARRPKRVKPVPEPVMVEDE
                     PELPDDEAATPMAPEPPGEGEYPDDPEDHFVADEDGTPDGKSEAAD"
     gene            5567..6196
                     /gene="purN"
                     /locus_tag="VFC01_09340"
     CDS             5567..6196
                     /gene="purN"
                     /locus_tag="VFC01_09340"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZN79875.1"
                     /translation="MQQSLRVPPSAPARLVVLASGTGSLLASLLEAAVGDYPARVVAV
                     GADRDCKALQIAADAGVPTYMVRLGDYSDRDAWDKSLTEATAAQEPDLVVSAGFMKIL
                     GPQFLSRFPGRIVNTHPALLPAFPGAHAVPAALAYGVKVTGCTVHLVDAGTDTGPILA
                     QQPVPVFDDDDEATLHERIKVVERRLLVDVLEALATRGVTWTGGKATIG"
     gene            6193..>7046
                     /gene="purH"
                     /locus_tag="VFC01_09345"
     CDS             6193..>7046
                     /gene="purH"
                     /locus_tag="VFC01_09345"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215472.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZN79876.1"
                     /translation="MTADEGRRKIRRALISVYDKTGLAELARGLHEAGVDIVSTGSTA
                     KTIAGTGVPVTPVEEVTGFPEVLDGRVKTLHPRVHAGLLADQRKPEHVSALADLGIAA
                     FELVVVNLYPFTETVDSGASIDECVEQIDIGGPSMVRAAAKNHPSVAVVVDPFGYDGV
                     LAAVRAGGFTIDERKRLASLAFRHTADYDVAVASWMGSVLAPEEPRVPMPAWLGATWR
                     REAVLRYGENPHQQAALYRNDAVWPGLAQAEQMHGKEMSYNNYTDADAAWRAAFDHED
                     ICVAIIKHA"
BASE COUNT         1121 a         2357 c         2382 g         1186 t
ORIGIN      
        1 gtcacggccg ccgcggtacc gaggctgacc caggggtcca gggtgcgcat gtagcgatcg
       61 tcgggcagcg actcgtcgcc ggtggtgggg tgggcagcct gccgcttgac cgggatgtgg
      121 gtgtgctcgg gcacgtagaa cgtggcaaag ccgtggtcat cggcgagctt cgccgccgcc
      181 gcaggggtga tgccgcggtc gctggtgaac agtacgagcc cgtaatccat gccaagaatt
      241 agaacgtgtt ccagtctggc gggcaagggc ggggtcccgt ttgcagctca gcagggcttt
      301 cgggaacgca caagtagttc gctaggtccg ttcgtggcag aaacgcgtac tcgactacgt
      361 atgtgcggac attcctggcg ggcgacagtt caggtgtgaa cagcggggcg acaacgccaa
      421 ccaaactgcg cgagtgggta cgccgctacc tgccgtgcga aatcgccgga acagtagggg
      481 aattgggcgg cgccgcggtc gcatatctgg cgaccggatc gctggccgcg gcggcgatca
      541 ccgcgaccat cggcgcgtcc gccggctact acgctgcggc ctacgtcaac gctctgcgct
      601 ggagctaccg cgaccacgct taccggcgtt ggccgtcacg gctactgatg tccaacttgc
      661 tcgcgctgcg cagcgtggcc gttgagttcg gcccggccga gctgatcgac agcattgcgg
      721 tcaggcccgt cgcgttctac gtcggcccgc tgatcttcga caacacgatc gtcggctgga
      781 tcttcggcaa gctcatctcc gacctggcgt tttacgtctg cgccatcgcc agctacgaac
      841 gcttcaccgg cctgctcgtg ggggcccggc cgcgcgccga ggaggtgacc gatgcatccg
      901 tcccgacgat cgaagctgcg tgaggacctg cgggcagata cgcagcgcct taccgcgctc
      961 gccgagcgcc acggcacacc gctgctggtg ctgcagccgc acctggtcgc ccgccgctat
     1021 cgagagctga cggcaaaact gcccggattc ggcatgcact acgccgtcaa ggcgtcaccg
     1081 catccgctcg tgctgtcgac catcgcgatc tgcggcggcg ggttcgacgt cgcctccagc
     1141 gccgaggtcg acctgctgca gcagctcggc ttgccgatgg accgctgcat ccacacccac
     1201 ccgatcaaga agcctgctga catcgaccac gcatacgcgg cgggcatccg cacgttcgtc
     1261 gtcgagaatc cttgcgaagc acagaaattc gccggccgac cggacgatat tgagctgctg
     1321 gtgcggctcg cgttccgcaa tccggccgcg aagtccgacc tgtcgtcgaa attcggagtc
     1381 gagccggccg aggccgaact actcgtcaaa cacgtgattg ccgccggtgt gcagttcgca
     1441 gggttcagct tccacgtcgg cagccagggc tcgtcggcgc agccctttga cacggccctt
     1501 cgcggcaccc ttgacctcgt cacggacatc gccgcgagcc tgggcgtgca cactcgcgtg
     1561 atcgacatcg gcggcgggtt ccccgttgcc taccgcgagt cgatgcccac cctcgacgag
     1621 atcgccggcg tgatcgacgg cgcgctgggc cctgcgcgtg acgacttcac gctcctcgca
     1681 gagccggggc ggtatctggt cgcggacagc atgacactgc tgaccagcgt cgtcggcacc
     1741 gcgcagcgcg acggtcagac gtggcactac ctcgacgacg ggatctacgg cagctactcc
     1801 aacgtgatga ccgaagacgt gcacccgccg atcctcgcgc tgcgtgacct cgccaatggc
     1861 gcgggcgaat tcgagccggt gaccctcgcc gggccgacgt gtgacagcgc agacgtgatc
     1921 gcccgcgact acccgatgcc cgctcttgag gtcggcgacg ttgtgatcag cccgatgatg
     1981 ggcgcctaca ccagcgtgac gtcatcgcgg ttcaacggca tcgcggccac gcccatcgtg
     2041 atgtcttagg ttcagcgcga gctttaccca cgacggccgg gccgcgctgc tgctgtcatg
     2101 gcacaccatg acgactgaac gcatcgcaga ccacgtcaag ttcgcttact gggtgccgaa
     2161 cgtcagtggc ggcctcgtca ccagcgacat cgagcagcgc accgattgga actacgacta
     2221 caacgccaag ctcgcccaaa ccgcggagaa caacgggttt gagtacgcgc tgtcgcaggt
     2281 gcgttacgag gccagctacg gcgcggagta tcagcacgaa tcgaccagct tctcgctggc
     2341 gctgctgatg gccacgcaga agctcaaagt gatcgccgcc gtccaccccg gactgtggca
     2401 gccgggcgtg ctggccaagc tcggcgccac cgccgaccac ctgtccggcg gccggttcgc
     2461 cgtcaacgtg gtttccggct ggttcaagga cgagttcacg cacctgggtg agccctggct
     2521 cgaacacgac gagcgctacc gccgcagcgc cgagttcctg caggtgctgc gcaagatctg
     2581 gaccgaggac aacgtcgact ttcgcggcga cttctaccgg atccacgact tcaccctcaa
     2641 gcccaaaccg ctcaacacgc ccgagcggcc caacccggag atcttccagg gcggcaactc
     2701 caccgccgca cgccgcaacg gcggttacta cgccgactgg tacttctcca acggcaagga
     2761 tttcgacgga ctcaccgaac aggtcgtcga ggtacgcgac catgcccgcg aggtcggtcg
     2821 tgaggtccgc ttcgggctca acggcttcat catcgcccgc gacaccgaaa aggaagccag
     2881 ggaagtcctc agggagatca tcgacaaggc caaccgtccc gcggtcgagg gattccgctc
     2941 tgcggtgcaa caggctggca actcgacgtt agacaagcgc ggtatgtggg ccgactcgac
     3001 gttcgaagac ttggtgcagt acaacgacgg cttccgtacg cagttgatcg gaacgcccga
     3061 gcagatcgcg gaacgcgttg cggcgtaccg caaacgcggt gtcgacctga tcctcggtgg
     3121 cttcctgcac ttccaggagg aaatcgaata ctttggcgcc aaggtgctgc cgttggtgcg
     3181 ggaaatcgaa gattccgaag aggattcggt cgattcgccg gtgctggtgt ccgcctgagg
     3241 cgccgatggc caccgccttc tcatcaggtt gcgctagcgt ggatgactga atcgctcagg
     3301 ccagcacagg agagtcatgt cttacccgcc cggcagcccc ggatacccac ccgcgcagca
     3361 gccgaccact cagttctcag cacccaccca gcagttcggc aagctgcccg agccggcagc
     3421 ccccgccgcc gcggaaggcc cgagcaagtt gcccgtgtac ctgaccgccg cagtggcggc
     3481 gcttgggctc gcggtgtacc tgtcgaacta cggtccactg ttcgaagcgg ccggtggcga
     3541 gttcttcacg cccacactgc tcgatctggg cgtggtggcg tcgctgcttg ccgcgctgat
     3601 cgccggcgtg ggcctgatcc ccaagcagaa gacgtcgccc gcgctggtgg cggtgctatc
     3661 ggtgctgggc ttcctgctgg tggtctcgat cgtgctcact gcgccgcagg gcgtgaccat
     3721 cgaatggggc ctgtacctga tcatcgcgtt cagcgtggtg caggcgattg tcgccgtcgc
     3781 ggtgctgctg ttcgacgcgg gtgtcatcgc gccgtccgtc ccgaagccgc gctacgagcc
     3841 gcagcagtac ggccagtacg gcggccaagg accgtattac ggacagcctc atcagccagg
     3901 cccgccgccg cagcagcagc agctgcgccc cggctatccg tcgccgtacc ccggcggcta
     3961 ccccggtggg ccgcccagcg gcggttattc cggcggccag cagagcggcc cgccgacgcc
     4021 gccgaccggt ttccccacct acgggcagcc gccgtcatcg agcaacgcgg gcaacgcggg
     4081 caacgcgggc aactcgagca gtgcgccgac gacacaggtg cccgtgcagc cgtcgtcttc
     4141 cgcacagtcc tccccgccgc cgtcgtagtc tgggccgcgc gtgcgtcgac ttcgcgcgcg
     4201 tgacctagcg cgaggagcgg ccccacccgt ggagaaccgg ccagtcggca cacgcaacgc
     4261 gcgtgagctg cttcgggtcg catttgggcc gtccgtcgta gcgctggtcg tcatcgctgc
     4321 ggtggtgttg ctgcagttgc tcatcgcgaa cagcgacatg accggcgccc ttggcgcgac
     4381 cgcgagcatg tggctgggtg tgcaccaggt gccggtgtcg atcggcggtc gcgagctcgg
     4441 cgtgatgccg ctgctgccag tgctggcgat ggtgtggggc accgcacgca cgacggcgtc
     4501 ggcgacgtca ccgcaggcct cgtggttcgt cacccgctgg gtggtcgcct cggcgctcgg
     4561 tggcccgctg ctgatcgccg cgatctcgct ggcggtcatc cacgacgcgg cgtccgtgct
     4621 gaccgaactg cagacaccta acgcgctgcg ggccttcgcc ggcgtgctgg gcgtgcacgc
     4681 tgtcggcgcg gtgatcgggg tcgggtcgcg cgtcggccgc cggacattga cggcgtcacc
     4741 gctgccgaag tggttgcccg acgcgttccg cgcggcgact gcgggggtgc tggcactgtt
     4801 cggcctctcg ggcgtggtga tggtcggttc gctggtcgtg cattggtcga cgatgcacga
     4861 cctgtacgcg atcaccgatt cagtgttcgg gcagttcagc ctgactgtgc tgtcggtgct
     4921 gtacgcgccg aacgtgatgg tcggcacggc cgcggtcgcg gtcgggtcga gcacgcatgt
     4981 cggcttggcc acgttcagcg cgttcacggt gttcggtggc gacatcccgg cgctgccggt
     5041 gttggcagcg gtgccgtcgc caccgctcgg ccccgtgtgg gtggccctgc tgatcgttgc
     5101 cgcggcttcc ggcgtcgcgg tggggcagca gtgcgcccgg cgtccgctgc cgctgctgcc
     5161 tgcgctgggc aagctggtcg tcgcgtccgc gtgtgccgcg ttggccatgg cgctgctcgg
     5221 attcgccggc ggtggtcggc tgggcaactt cggtgacgtc ggcgtcgacc aggtcacgtt
     5281 cgcacccgcg gtgttcctgt ggttcgtcgg catcggtgcg ctgacggtcg cgatgtcggg
     5341 cggtattgcg cgacgcccca agcgggtcaa gccggtgccc gagcccgtga tggtcgagga
     5401 cgagcccgaa ttgcccgacg atgaggccgc aacaccgatg gcgccggagc cgccgggaga
     5461 gggagagtat ccggacgacc cggaggacca cttcgttgct gacgaggacg gcacgcctga
     5521 cggtaagagt gaagccgccg actgagcacc cactattgtt cgaggcgtgc agcaatcgct
     5581 ccgcgtgccc ccgagtgcac cggcgcggct cgtcgttctg gcgtccggaa ccggttcatt
     5641 gctcgcctct ctgctggaag cggccgtcgg cgactacccg gcgcgggtgg tggccgtcgg
     5701 cgcggaccgt gactgcaagg ccctgcagat cgctgcggac gcgggcgtgc cgacatacat
     5761 ggtgcggctg ggcgattaca gcgaccgcga cgcgtgggac aaatcgctga ccgaggcgac
     5821 cgcggcgcag gagcccgatc tcgtggtatc ggccgggttc atgaaaatcc ttgggccgca
     5881 gtttctttcc cggtttcccg gtcgcatcgt gaacacccat ccggcgttgc tgcccgcatt
     5941 tcccggcgcg cacgccgtgc ccgcggcgct cgcgtatggc gtcaaagtga ccggctgcac
     6001 cgtgcatctc gtcgacgcag gaaccgacac cgggcccatc ctggcccagc aaccggttcc
     6061 ggtcttcgac gacgacgacg aagccaccct gcacgagcgg atcaaggtcg tcgaacgacg
     6121 gctgctggtg gacgttttgg aagcgctggc cacccgcggc gtgacctgga ccggaggaaa
     6181 ggcgacaata ggatgaccgc agatgagggg cgcaggaaaa tccgccgtgc actgatcagc
     6241 gtctacgaca agaccgggct ggccgagttg gcccgtggcc tgcacgaagc gggcgtcgac
     6301 atcgtgtcga cgggttcgac cgccaaaacc attgcgggca ccggggttcc ggtcacgccc
     6361 gtcgaggagg tcaccggctt tcctgaggtg ctcgacggcc gggtcaagac cctgcacccg
     6421 cgcgtgcacg ccggtctgct cgccgatcag cgcaaacccg aacatgtttc ggcgctcgcc
     6481 gacctcggca tcgccgcgtt cgaactcgtg gtcgtcaacc tgtatccgtt caccgagacc
     6541 gtcgactccg gcgccagcat cgacgagtgc gtcgaacaga tcgacatcgg aggcccgtcg
     6601 atggtgcgcg ccgccgccaa gaaccatccc agcgtggccg tggtcgtcga tccgttcgga
     6661 tacgacggtg tgctcgccgc ggtgcgggca ggcggattca cgatcgatga gcggaagagg
     6721 ctggcgtcgt tggctttccg gcacaccgcc gactacgacg tggcggtggc gtcgtggatg
     6781 gggtcggtgc tcgcgcctga ggagcctcgg gtgccgatgc cggcatggct tggtgcgaca
     6841 tggcgccgcg aggcggtact gcgctacggc gagaacccgc atcagcaggc cgcgctgtac
     6901 cgcaacgacg ccgtgtggcc gggattggct caggccgagc agatgcacgg aaaagagatg
     6961 tcctacaaca actacacgga tgccgacgct gcgtggcggg ccgcgttcga ccacgaagat
     7021 atctgcgtgg cgatcatcaa gcacgc
//
LOCUS       DASRJA010000209         7028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3248329,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000209 DASRJA010000000
VERSION     DASRJA010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3248329"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFC01_09350"
     CDS             complement(<1..917)
                     /locus_tag="VFC01_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZN79877.1"
                     /translation="MRQRRFSVLVGAILLLLAGCGGQRGGDNGGSAAEPTSCEVTADA
                     RVSIATGNTTGVYYTLGGAYAEAISQQTGGKLKATAAETSASVQNIQQLVAGTHQIAF
                     SLADTAVDAVNGSASFKEKQPITALTRLYSNYTQVIARTNAGINTIADMRDKRVSTGA
                     PNSGTEVIANRMLEAAGLDPGKDIQAQRTELGKTVEGMKDGSIDAMFWSGGLPTGGIT
                     DLFVSRRDQVKFIDVTDTLAKLKEINPIYEQGVILASTYQTPADVPTVVVPNVLLVKD
                     DMDGNTACVLTKALFDHKADLVKANKAADG"
     gene            complement(979..1884)
                     /locus_tag="VFC01_09355"
     CDS             complement(979..1884)
                     /locus_tag="VFC01_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN79878.1"
                     /translation="MGLLDGRVVIVTGAGGGIGRAHALAFAAEGARVVVNDIGVGLDG
                     SPAGGGSAAQGVVDEITAAGGEAVTSGANVADWQQAEGLIQTAIDSFGGLDVLVNNAG
                     IVRDRMFANTSEEEFDAVIAVHLKGHFATMRHAAAYWRAQSKAGNSVDARIINTSSGA
                     GLQGSVGQANYSAAKAGIAAMTLVASAEMGRYGVTVNAIAPSARTRMTETVFAEMMAT
                     QDKDFDAMAPENISPLVVWLGSVESKEITGRMFEVEGGVVRVAEGWAHGPAVDKGARW
                     DPAELGPVVKDLLAKARPPVPVYGA"
     gene            complement(1899..2669)
                     /locus_tag="VFC01_09360"
     CDS             complement(1899..2669)
                     /locus_tag="VFC01_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN79879.1"
                     /translation="MTDAADINLGLKDRVVLVTGGVRGVGAGISAVFADQGATVVTCA
                     RRPVDGLPYEFHSCDVRDEDAVAALIKTIVERHGRLDVVVNNAGGSPYVLTADASAKF
                     NRKIIELNLIGPLSVSQHANAYMQKQPGGGSIVNISSVSGRRPTPGTAAYGAAKAGID
                     SLTSTLAVEWAPKVRVNSVVVGMVETEQSELFYGDAESVAAVAATVPLGRLAKPTDIG
                     WAAAFLASEAASYISGATLEVHGGGEPPSYLEASSANK"
     gene            2722..3480
                     /locus_tag="VFC01_09365"
     CDS             2722..3480
                     /locus_tag="VFC01_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HZN79880.1"
                     /translation="MPITSTTTEPGIVSVTVDYPPVNAIPSRGWFELADAITKAGEDQ
                     QTHVVILRAEGRGFNAGVDIKEMQKTEGFTALIDANRGCFAAFRAVYECAVPVVAAVN
                     GFCVGGGIGLVGNADVIVASDDATFGLPEVERGALGAATHLSRLVPQHLMRRLFFTAA
                     TVDAATLHHFGSVHEVVPRSELDEAALRVARDIAAKDTRVIRAAKEALNLIDVQKVNS
                     SYRMEQGFTFELNLAGVADEHRDAFAGTAKGKQA"
     gene            3477..4379
                     /locus_tag="VFC01_09370"
     CDS             3477..4379
                     /locus_tag="VFC01_09370"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZN79881.1"
                     /translation="MSDKRTTLDDAVSSIESGMTIGIGGWGSRRKPMAFVRALLRTPV
                     KDLTVVTYGGPDLGLLCSAGKVRRVYYGFVSLDSAPFYDPWFAKARTSGAIEAREMDE
                     GMLRCGLQAAAQRLPFLPIRAGLGSDVRAFWGEELKTVTSPYPTDGDFEELIAMPALR
                     LDAAFVHMNLGDAQGNAAYTGIDPYFDDLYLMAADRRYLSVERVVPTEELVKAVPPQA
                     LLINRMMVDSVVEAPNGAHFTTAAPDYGRDEKFQRHYAEAAASDESWAGFVQTYLSDS
                     EADYQAAVRKFWTADARSGKAGAQ"
     gene            4376..5125
                     /locus_tag="VFC01_09375"
     CDS             4376..5125
                     /locus_tag="VFC01_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZN79882.1"
                     /translation="MISVSRPEVCAVACAELFRDAGEIMVSPMATMVSIGARLARLTF
                     SPDILLTDGEAQLLADTPAIGAVGAIEGWMPFGRVFETLAWGRRHVVMGANQIDRFGN
                     QNLSAFGPLQHPTRQMFGVRGAPGNTINHATSYWVGNHSKRVFGDSVDVVSGIGWDKV
                     DRDNPAYRFVNVYRVVSNLGVFDFNGPDHQMRAVSLHPGITPEEVAENTSFEVHDLDK
                     VEQTRLPTADELKLLREVIDPKSLRDKEVRA"
     gene            5122..6183
                     /locus_tag="VFC01_09380"
     CDS             5122..6183
                     /locus_tag="VFC01_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZN79883.1"
                     /translation="MSKLRTPLTELVGIEHPVVQTGMGWVAGARLVSATANAGGLGIL
                     ASATMTLDELQAAVTKVKAATDKPFGINIRADAGDANDRIDLLIREGVKVASFALAPK
                     PDLIAKLKDAGVVVIPSVGLAKHAKKVADWGADAVIVQGGEGGGHTGPIATTLLLPSV
                     LDAVDIPVVAAGGFFDGRGLAAALSYGAAGVAMGTRFLLTSDSTVPDAVKQRYLEAGL
                     DGTVVSTRVDGMPHRVLRTGLVEKLESGSRLRGFSAAVRNAQKFKKMSRMTWRSMISD
                     GLEMRHGKELTWSQVVMAANTPMLLKAGLVEGNTDAGVLASGQVAGILDDLPSCAELI
                     DAIVADAVKHLQAAAAFVE"
     gene            complement(6250..>7028)
                     /locus_tag="VFC01_09385"
     CDS             complement(6250..>7028)
                     /locus_tag="VFC01_09385"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZN79884.1"
                     /translation="SSAMTLAEPLGFKDPFSGSEGWVARYGPAEVSQFRGAEMIAEKW
                     NISREEMEQYALTSHQRAQAAIRAGHFENEIIEVDGFSTDEGPRDTSLEKMATLPTLV
                     EGGRLTAAMASQISDGASAVLLASEQAVKEHNLRPRARIHHISARGADPVFMLTGPIP
                     ATRYALEKTGLSIDDIDTVEINEAFAPVVQAWLKEIKADPEKVNPNGGAIALGHPLGA
                     TGAKLFATMLNTLERTGGRYGLQTMCEGGGTANVTIIERL"
BASE COUNT         1152 a         2246 c         2364 g         1266 t
ORIGIN      
        1 ccgtcagcgg ccttgttcgc cttgaccagg tcggccttgt ggtcgaacag cgctttggtc
       61 agcacgcagg cggtgttgcc gtccatatcg tctttgacca gaagcacatt gggcaccacc
      121 acggtcggca cgtccgcggg cgtctggtag gtgctggcca ggatgacacc ctgctcgtag
      181 atggggttga tctccttcag cttcgccagg gtgtcggtca cgtcgatgaa tttgacttgg
      241 tcgcgtctgc tgacgaacag gtcggtgatg ccgccggtgg gcaggccgcc ggaccagaac
      301 atcgcgtcga tcgagccgtc cttcatgcct tcgacggtct tgccgagttc ggtgcgctga
      361 gcctgaatgt ctttcccggg gtcgagtccg gccgcttcga gcatccggtt ggcgatgacc
      421 tccgtgccgg agttcggcgc cccggtggac acccgcttgt cgcgcatatc ggcgatcgtg
      481 ttgatgccgg cgttggtgcg ggcgatcacc tgcgtgtaat tggagtacag gcgggtgagt
      541 gcggtgatcg gctgtttctc cttgaacgac gcgctgccgt tgacggcgtc gacggcggtg
      601 tccgcaagcg agaaagcgat ctgatgggtg cccgctacca gctgctggat gttctgcact
      661 gacgcggatg tctccgcggc ggtcgccttg agcttgccgc cggtctgttg gctgatcgcc
      721 tcggcgtacg cgccgccgag cgtgtagtaa acccccgtcg tgttcccggt cgcgatgctc
      781 acccgcgcat cggcggtcac ttcgcacgag gtcggctcgg ccgcggaccc cccgttgtcg
      841 ccgccgcgct gaccgccaca accggccagt agcaacaaga ttgcgccgac aagaacagaa
      901 aatcgtcgct gtctcatcgg tccatcatgg tccgaggcgg gatggttcag cggcggttca
      961 gccggaatta gtcaggcatt aggcaccgta gaccggaacc ggcggccgag ccttggcgag
     1021 cagatccttg accaccggac ccagctcggc cggatcccac cgggcaccct tgtccaccgc
     1081 aggcccgtga gcccagccct cggccacccg gacgacgccg ccctcgactt cgaacatcct
     1141 gccggtgatc tccttcgact cgacgctgcc gagccaaacc accagcggtg aaatgttttc
     1201 cggcgccatt gcgtcgaagt ctttgtcctg ggtcgccatc atctcggcga agaccgtctc
     1261 ggtcattcgg gtgcgggcag acggggcgat cgcgttgaca gtgacgccgt agcggcccat
     1321 ctccgcagag gccaccagcg tcatcgctgc gatgcccgcc ttcgcggcgc tgtaattggc
     1381 ctgtcccacg ctgccctgca gacccgcgcc ggaactggtg ttgatgatgc gcgcgtcaac
     1441 gctgttaccc gccttggact gggcgcgcca gtaggccgcc gcgtgccgca tggtggcgaa
     1501 gtggcccttg agatggacgg cgatgaccgc gtcgaactct tcctcgctgg tgttggcgaa
     1561 catgcggtcg cgaacgatgc ccgcgttgtt gaccagcacg tccaggccgc cgaagctgtc
     1621 gatcgccgtc tggatcaggc cttcggcttg ctgccagtcg gcgacattcg ccccactggt
     1681 gactgcctca ccaccggcgg cggtgatctc gtcgacaaca ccctgcgcgg cgctgccgcc
     1741 tcccgcgggc gacccgtcga gtcccacgcc gatgtcgttg accaccacac gcgcgccctc
     1801 ggcggcgaac gccagcgcgt gcgcacgccc gataccgccg cccgcacccg tcacgatgac
     1861 cacacggccg tcgagcaaac ccatatcgcg tgtctccttt atttgttggc gctcgacgct
     1921 tccagatacg acggcggttc gccgccacca tggacctcga gcgtggcgcc gctgatataa
     1981 gacgccgctt cggatgccaa aaaagctgcg gcccaaccga tatcggtggg cttggccagt
     2041 cggcccagcg ggacggtggc cgcgacagcg gcgacggact ccgcgtcacc gtagaacagt
     2101 tcggattgtt cggtttccac cattccgacc acgaccgaat tcacccgcac cttgggcgcc
     2161 cactcgaccg caagggtgct ggtgaggctg tcgatgccgg ccttggcggc gccgtacgcc
     2221 gccgttcccg gggtgggtct gcggccgctg acgctggaga tgttgacgat cgagccgccg
     2281 ccgggctgct tctgcatgta cgcgtttgca tgctgcgaca ccgacaacgg cccaatcaaa
     2341 ttgagctcga tgatcttccg gttgaacttc gcgctggcgt cagcggtgag gacatacggg
     2401 gagccgcctg cgttgttcac cacgacatcg agccgcccgt gccgctcgac gatcgttttg
     2461 atgagcgcgg cgacagcgtc ctcatcgcgc acgtcgcagg agtggaactc gtagggcagc
     2521 ccgtcgacgg ggcggcgggc acacgtcacc accgtggcgc cctggtcggc gaagacggcg
     2581 ctgatgccgg cgccgacgcc gcggactcca ccggtcacga ggaccaccct gtccttgagt
     2641 ccgaggttta tgtcagcggc gtcggtcact gtgctagcgt accaagcaag tgcttgctta
     2701 ggtaccgacc caggaagtgc aatgccaatc acgtccacga ccacggaacc gggcatcgtc
     2761 tcggtcaccg tcgactaccc gccggtcaac gcgattccgt cgcgcggctg gttcgaactc
     2821 gccgacgcca ttaccaaggc gggcgaagac caacagacgc acgtcgtgat cctccgcgcg
     2881 gaggggcggg gtttcaacgc cggcgtcgac atcaaggaga tgcagaagac cgagggcttc
     2941 accgcgctca tcgacgccaa ccgcggctgc ttcgcggcgt tccgcgcggt ctacgaatgc
     3001 gcggtgccgg tagtagcggc ggtcaacggc ttctgtgtcg gcggtggcat cggcctcgtc
     3061 ggcaacgccg acgtgatcgt cgcctctgac gacgcgacgt tcgggctgcc ggaggtggaa
     3121 cgcggcgctt tgggggcggc gacgcatctg tcgcggctgg tgccccagca cctgatgcgc
     3181 aggctgttct tcaccgcggc caccgtcgac gccgcgaccc ttcaccattt cgggtccgtg
     3241 cacgaagtcg tgccgcgctc tgagttggac gaggccgcgc tgcgggtcgc gcgcgacatc
     3301 gccgccaagg acacccgggt gatccgcgcc gccaaggaag ccctcaatct gatcgatgtc
     3361 cagaaggtga actccagtta ccgcatggaa cagggcttta cgttcgagct gaacctcgcc
     3421 ggtgtggcgg acgaacaccg cgacgcgttc gccggcactg cgaagggcaa gcaggcatga
     3481 gcgacaagcg gaccacgctc gacgatgctg tgtcgtcgat cgagagcggg atgacgatcg
     3541 gcatcggcgg ctggggatcg cggcgcaagc cgatggcgtt cgtgcgcgcg ttactgcgca
     3601 ctccggtgaa ggacttgacg gtggtgacgt acggcgggcc ggatctcggt ctgctgtgtt
     3661 cggcgggcaa ggtgcggcgc gtgtattacg gcttcgtgtc gttggattcg gcaccgttct
     3721 acgatccgtg gttcgccaag gcgcgcacca gcggtgcgat cgaggcccgc gagatggacg
     3781 agggcatgct gcgctgcgga cttcaggcgg cggcgcagcg gctgccgttc ctgccgatcc
     3841 gcgccggcct tggcagcgac gtgcgcgcct tctggggtga ggaactgaag actgtgacgt
     3901 cgccctatcc gacggacgga gacttcgagg aactcatcgc gatgcccgcg ctgcgacttg
     3961 acgccgcgtt cgtgcacatg aatctcggtg atgcacaagg caacgccgcc tacaccggca
     4021 tcgacccgta cttcgacgac ctgtacctaa tggccgctga caggcgttat ctgtcagtgg
     4081 agcgggttgt gcccaccgag gaactcgtca aggccgttcc accgcaggcg ctgctgatca
     4141 accggatgat ggtggactcc gtcgtcgaag cgcccaatgg cgcgcatttc accaccgctg
     4201 cacccgatta cggtcgcgac gagaaattcc agcggcacta cgccgaggcc gccgcgtccg
     4261 acgaatcatg ggccggcttt gttcagacct atctgtccga cagcgaggcc gactatcagg
     4321 ccgcggtgcg aaagttttgg accgcggacg cgaggagcgg aaaggcagga gcgcagtgat
     4381 ttccgtgagc cgtccggagg tgtgtgcggt cgcctgtgcg gaattgttcc gcgacgcggg
     4441 cgagatcatg gtgagcccga tggcgacgat ggtgtcgatc ggtgcgcgac tggcgcggct
     4501 gacgttctcc cccgacatcc tactgaccga cggcgaggcg caattgctgg ccgacacgcc
     4561 tgcgatcggg gcggtcgggg cgattgaagg ttggatgccg ttcggcaggg tcttcgagac
     4621 gttggcctgg gggcgtcgtc atgtggtcat gggcgccaat cagattgacc gtttcggcaa
     4681 tcagaacctg tcggcattcg ggccgctgca gcacccgacc cggcagatgt tcggtgtccg
     4741 tggcgcgcca ggcaacacga tcaatcacgc gacgagctac tgggtcggca accactccaa
     4801 gcgggtgttc ggcgattcgg tcgacgtcgt gtcgggcatc ggctgggaca aggtcgatcg
     4861 tgacaacccg gcgtaccgct ttgtcaatgt gtaccgggtg gtgtccaatc tcggggtctt
     4921 cgacttcaac gggccggatc accagatgcg ggcggtgtcg ctgcatccgg gcatcacacc
     4981 ggaagaggta gcagagaata cctcgttcga ggtgcacgat ctggacaagg tcgagcagac
     5041 gcggcttccc accgctgacg agctgaagct gctgcgcgag gtgatcgatc cgaagtcgct
     5101 gcgggacaag gaagtacgag catgagcaag ctgcgcacgc cgctgaccga gctggtcggt
     5161 atcgagcatc cggtggtgca gaccgggatg ggctgggtgg cgggtgcgcg gctggtgtcg
     5221 gcgaccgcca acgccggcgg cctgggaatc ctggcatcgg cgaccatgac gctcgacgaa
     5281 ctgcaggcgg cggttaccaa ggtcaaggcg gcgacggaca agccgttcgg catcaacatc
     5341 cgtgccgacg cgggcgatgc caacgatcgc atcgacctgc tgatccgcga gggcgtgaag
     5401 gttgcgtcgt tcgcgttggc gccgaagccg gatctgatcg cgaagctcaa agacgctggc
     5461 gtggtggtga ttccctcggt aggcctcgca aagcatgcga agaaagtggc cgactggggt
     5521 gccgatgcgg tgatcgtcca gggcggcgag ggcggcgggc acacgggtcc gattgcgacc
     5581 acactgctgc tgccgtcggt gctcgacgcg gtagacatcc ccgtcgtcgc ggcgggcggt
     5641 ttcttcgatg gccggggctt agcagcggcg ttgtcgtatg gcgcggcggg cgtcgcgatg
     5701 ggcacgcgat tcttgttgac ctccgattcc accgtgccgg atgccgtcaa acagcgctat
     5761 ctcgaggcgg ggctcgacgg tactgtggtg tccacccgcg tcgacggcat gccgcaccgg
     5821 gtgctgcgca ccggcttggt cgagaagctc gaaagcggtt cgcggctgcg aggtttctcg
     5881 gcggcggtcc gcaacgcaca gaagttcaag aagatgtcgc gcatgacctg gcggtcgatg
     5941 atcagcgacg ggctcgagat gcgacacggc aaggagctga cgtggtcgca ggtcgtgatg
     6001 gcggccaata ccccgatgct gctgaaagcc gggctggtcg aaggcaacac ggacgccggc
     6061 gtgctcgcgt cgggtcaggt cgcgggcatt ctcgacgatc tgccatcgtg cgctgagctg
     6121 atcgacgcga tcgtggccga cgccgtaaag catctgcagg cggcggccgc gttcgtcgaa
     6181 taaccgccga aactgtattc cagcagcgaa agttcgcgta gaggcctgct ggaatacagt
     6241 ttcgcgggac tacaggcgct cgatgatggt gacgttggcg gtgccgccgc cctcgcacat
     6301 cgtctgcaga ccgtagcggc cgcccgtacg ctcaagcgtg ttgagcatgg tggcgaacag
     6361 cttggcccct gttgcgccga gcggatggcc caacgcgatc gccccgccgt tcgggttgac
     6421 cttctcggga tccgccttga tctccttgag ccacgcctga accaccggcg cgaaagcctc
     6481 gttgatctcg acggtatcga tgtcgtcaat cgaaagcccg gtcttctcaa gggcataacg
     6541 cgtcgcagga atcgggccgg tcagcatgaa caccgggtcg gcaccgcgag cgctgatgtg
     6601 gtggatccgc gcccgcggcc tcaggttgtg ttccttgacc gcctgctcag acgccaggag
     6661 tacggcactc gcgccatcgg agatctggct cgccatcgcc gcggtcaatc ggccaccctc
     6721 aaccagggtg ggcaaggtcg ccatcttctc caacgacgtg tcgcgcgggc cctcgtcggt
     6781 actgaatccg tcgacctcga tgatctcgtt ctcgaagtgg ccggcgcgaa tcgcggcctg
     6841 ggcccgctgg tggctggtca gcgcgtactg ctccatctcc tcgcgcgaga tgttccactt
     6901 ctccgcgatc atctcggcac cgcggaactg cgacacctcg gcggggccgt agcgcgccac
     6961 ccagccctcg gatcccgaga acggatcctt gaagccgagc ggctcggcca gcgtcatcgc
     7021 cgacgaga
//
LOCUS       DASRJA010000210         5812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_995516,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000210 DASRJA010000000
VERSION     DASRJA010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995516"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..1225
                     /locus_tag="VFC01_09390"
     CDS             395..1225
                     /locus_tag="VFC01_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Emopamil-binding protein"
                     /protein_id="HZN79885.1"
                     /translation="MTTLEEEHDFAKPWAKRRVKTYAWCSAFTATVAVGSIIGVGVGW
                     IPMVPLTGVIACFLFMTPVFVMFGASWINAPGETRTTLEKANEFQMLWFCIAGAASEL
                     WWEVAWLVGDLAGWMHITDQQRWGWVFWYYGINDVRYLNSDGPLWAMELVVVIGAAVL
                     LFSWWKLRNAGNDPDKRIRPLWWSFATMSAMLTVFFIYFVAEARHGFPNFPRHGFWDV
                     SIILIYENLPWMIAPVVSLPFVAKQLGYLYGKRAQQAASVGSNRQTAQPPVAVGAHSN
                     "
     gene            1352..2419
                     /locus_tag="VFC01_09395"
     CDS             1352..2419
                     /locus_tag="VFC01_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN79886.1"
                     /translation="MPERVLVTGACGLVGSATVARLCKDGIPVVATDLDTAANRRAAK
                     RFGDAITQWCDLTQRSHVQRLLAAVRPAAIIHLAAVIPPFCYIQPATAYRVNVEATAL
                     LVQEASALTAPPRFVQASSVSVYGPRNPHIESEALTSNTRPAPFDVYGAQKVAAEGVV
                     QQSNLDWVILRLGGVMGPLLRHAKPDLAAMEGMLPSDGRIHMVDVRDAARAFVNAVTK
                     PIAHVRGKTLVIVGDSSYQLLQEQIAPSIMAALGLVGVMPQGRKGDPNSTTAWFTTDW
                     ADPDEAQRTLQFQHTTWPDLLAERADRARWLQPLGRTASPVARQVLRSRAPYRGQPGA
                     FADPWHMISQRWGSPLAQTTR"
     gene            complement(2776..2970)
                     /locus_tag="VFC01_09400"
     CDS             complement(2776..2970)
                     /locus_tag="VFC01_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79887.1"
                     /translation="MLREEGITIGRQGLTSEQSAEAEMLYSDGKSLAWLGARYDVSHT
                     TVAAALRRQGMQLRPRPGWS"
     gene            3281..4357
                     /locus_tag="VFC01_09405"
     CDS             3281..4357
                     /locus_tag="VFC01_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016895786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZN79888.1"
                     /translation="MASIHAQKNRAANTYRVLWRQDGRQRSLTFENLPAAERFKTLLE
                     DHGPDEALRIIELDEIGRHVPTVTQWLHTHIDNLTGVQPATIARYRTYVARDIDPVFG
                     SMPVSAVTETTIAKWVKQLGGSGKTIANKHGFLSGALNSAVRAGVIATNPCQGRRLPH
                     TRVEETVFLTPDEFRLLRDHIERERWKNLATWLVTTGMRFSEATALTAADIDTDAKTC
                     RINKAWKYSGDYRPQIGPPKTKKSIRTISLPSAALDVLDLSAAGFLFTNGAGNPVRAQ
                     EFFHGGWKPGRENAMKAGLTKSPRVHDLRHTCASWMIQAGVPLPVIQQHLGHESIQTT
                     IGVYGHLDRRSAQVAADAIGSALS"
     gene            complement(4396..5020)
                     /locus_tag="VFC01_09410"
                     /pseudo
     CDS             complement(4396..5020)
                     /locus_tag="VFC01_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217705.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
     gene            complement(5017..5364)
                     /locus_tag="VFC01_09415"
     CDS             complement(5017..5364)
                     /locus_tag="VFC01_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HZN79889.1"
                     /translation="MAAALDRAVEASEIVDALKPFGVTQLDVAAVTQVSDRAVRGWRT
                     GDIRPDRYDRLAQLRDLVLLLSDSLTPRGVGQWLHAKNRILDGARPVELLAEDHYERV
                     RHAAESFIDGSYV"
     gene            5533..5812
                     /locus_tag="VFC01_09420"
                     /pseudo
     CDS             5533..5812
                     /locus_tag="VFC01_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470225.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT         1108 a         1805 c         1835 g         1064 t
ORIGIN      
        1 cgacacatcg gcggtccggg cgatgtcggt gaccttcgtc ttgtcgaagc ccgctctcgc
       61 gaactcgaac tctgcggcag agatgatctg ctggcggtac atatcgcggc gtgcatcgcg
      121 aatattcgga actgtcacga atcctgggct accacgtggg accacctctc ggctgagggt
      181 ctgacgttcg aatcataggc aggtcagcgg caggcgtgga ctgaccgctg cccacccggc
      241 gcgcatgaca gcttgtcgcc aggctggatg cgatttacgg cggaacagcc cattgcgctt
      301 catcgaacac actgctaatt tagcagaatc gcaattctgt ttaacagtac cactgttctg
      361 agcggctcag acgagcaaat cgaaagggac taacgtgacg acgctcgaag aagaacacga
      421 cttcgccaag ccatgggcca agcggcgcgt caagacctac gcctggtgct cggcgttcac
      481 cgccacagtc gccgtcggaa gcatcatcgg tgtcggggtg ggctggattc ccatggtgcc
      541 cctgacagga gtcattgcct gcttcctgtt catgacgcca gttttcgtca tgttcggggc
      601 atcttggatt aacgctccgg gcgagacgcg aaccacgttg gagaaagcca acgagtttca
      661 gatgctgtgg ttttgcatcg ctggcgcggc aagcgagttg tggtgggaag tggcctggct
      721 ggtcggcgac cttgcggggt ggatgcacat caccgatcag cagcgctggg gatgggtctt
      781 ttggtattac ggcatcaacg acgtgcgcta cctcaatagc gacgggccgt tgtgggccat
      841 ggaactggtc gtcgtgatcg gtgctgccgt tttgctgttc tcttggtgga agctgagaaa
      901 tgcaggcaac gaccccgaca agcgcatccg gccgctgtgg tggagcttcg cgaccatgtc
      961 ggcgatgttg acggtgttct tcatctactt cgtcgccgaa gcacgccacg ggttccccaa
     1021 ctttccgcgc catggattct gggacgtctc aatcatcctg atctacgaga acctcccgtg
     1081 gatgatcgcc ccggtcgtca gcctcccgtt cgtcgccaag caactgggtt atctctacgg
     1141 aaagcgggca caacaagcag cttccgttgg atcgaacaga cagaccgctc agccccccgt
     1201 tgccgttggt gcacatagca actgaccggt agagacggct cattccggct tgcgtgctgc
     1261 tcgcgctggt gaacacgaca ccagtaacgg aaccggcccg catgccggga tggctgccgt
     1321 gcgggctggc ggagaagaac aggagggccg gatgccagag cgagtactcg tgaccggtgc
     1381 atgcggcttg gtcgggtcgg ccaccgttgc acgattgtgc aaagacggca taccggtcgt
     1441 cgccaccgat ctagacacgg cggcaaatcg aagagccgcc aaacggtttg gcgatgccat
     1501 cacgcaatgg tgtgacctca cccagcgatc ccacgtccag aggctgctcg cagccgttcg
     1561 tccggcggcg atcatccacc tggcggcggt gataccccct ttctgctaca tacagccggc
     1621 caccgcatac agagtcaacg tcgaggcaac cgcacttctg gtccaggaag catcagcgtt
     1681 gactgcgcca ccgcggttcg tgcaagcgtc aagcgtgagc gtgtacggac cgcgcaatcc
     1741 acacatagag agcgaggcgc tgacctctaa cacccggccc gcgccgttcg acgtgtacgg
     1801 agcgcaaaaa gttgcggccg aaggcgttgt ccaacaatca aatctggatt gggtgatact
     1861 gcgcctcggc ggagtgatgg gaccgctact acgccatgcc aagcctgacc tcgcagccat
     1921 ggagggcatg cttcccagcg acggacgaat tcacatggtc gacgtccgcg acgctgcgcg
     1981 tgcgtttgtc aacgcggtaa ccaaacccat cgcacacgtc cgcggtaaga cactggtcat
     2041 cgtcggcgac agcagctacc agctactcca ggaacagatc gcaccgtcca tcatggctgc
     2101 cctcggcctt gtcggggtca tgccccaagg ccgaaagggc gaccccaaca gcaccaccgc
     2161 atggttcacc accgactggg ccgatcccga cgaggcacaa cgaacactcc agtttcagca
     2221 cacaacctgg cctgacctac tcgccgaaag ggccgaccgc gcccgatggt tacagccgtt
     2281 ggggaggacc gcttcgccag ttgcccgaca ggtgttacgt agccgtgcgc cataccgcgg
     2341 tcagcctggc gcattcgccg acccatggca catgatttcc cagcgatggg gaagccccct
     2401 ggcacagaca acacgttgag tgtgaagcca gttgacggcg aatgcaattc gcggacctcc
     2461 gcagttcgcg gttagtacac gcgcctacga ccaaccggag caacacttga acactgtccc
     2521 ctggcaccgg tgtaaccgct cctggcctcc ggaatcattc gcatggcgaa gcgcacgcaa
     2581 aaccagctag ccgctcatcc tccggctcct tcgcgaggcg tcggagacca aacgaccgac
     2641 ggataggcga cggcgacacc gttcttcgca ttcggatcgt gcgagagacg agccgcttgg
     2701 tcccgtaacg tgccgaagac aacgtgtgtg ttgacgccgt caacaccctt tgtgtgtctc
     2761 caaggagagc gaggcttaac tccaacctgg gcggggccgc agttgcatgc cttggcgccg
     2821 aagcgctgct gcgacggtcg tatgggagac gtcgtagcgg gcgccaagcc aggcgagtga
     2881 cttgccgtcg gagtagagca tttctgcttc tgccgattgc tcgctggtca ggccttgacg
     2941 gccgatggtg atgccctcct cacgaagcac ggtggccacc ctggacttgg agatcctgaa
     3001 gtgggcgccg atctgttggg tggtttcgcc ggcgtggtac cgatggacga tgtcatcgat
     3061 gtcggtgggg agcagaaagc gcttcgatcg ccggggagtg ccactggacg ctggtcgctg
     3121 gggtgcttcg ttgatgcgtt tacgcagttt agaccatctt ttacagaggt gactgaggtt
     3181 cgagtttctt cctattagct ccaccatata ttcgcagctc aaacgtggtt tttgaagtta
     3241 cgccgggttt gacgtaacac ttcacgtaac accgagggtc gtggcaagca ttcacgccca
     3301 gaaaaaccgc gccgcaaaca cctaccgcgt gctgtggcgc caggacggcc gccagcgctc
     3361 gctgaccttc gaaaacctcc ccgccgcaga acgtttcaag actctgctcg aagaccacgg
     3421 gcccgatgaa gcgctgcgga tcatcgagct cgacgagatc gggcggcacg tcccgacggt
     3481 cacccagtgg ctgcacaccc acatcgacaa cctgaccggt gtccaaccgg ctacgatcgc
     3541 tcgttaccgc acctatgtcg cccgggatat cgacccagtg ttcggctcca tgccggtgtc
     3601 ggcggtcacc gagacgacaa tcgcgaagtg ggtcaagcag ctcggcggaa gcggcaaaac
     3661 gatcgcgaac aagcacggct tcctatcggg cgcgctcaac tcggcggtac gtgctggcgt
     3721 gatcgcaacc aatccgtgcc aaggtcggcg cctgccccat acgcgtgttg aggagacggt
     3781 gttcctcacg cctgacgagt tccggctgct gcgcgatcac atcgagcggg aacggtggaa
     3841 gaacctggcc acctggctcg tcactaccgg tatgcgcttc tccgaggcga ccgctctgac
     3901 tgcggccgac atcgataccg acgcgaagac atgtcgaatc aataaggcgt ggaagtactc
     3961 cggcgattat cggccgcaga tcggtccgcc gaagaccaag aaatcgatcc gcaccatcag
     4021 cctgccgtca gccgccctgg acgttctgga cctttctgcg gccggctttc tattcaccaa
     4081 cggcgccggc aatccggttc gcgcacaaga gttcttccac ggcggctgga aacccggccg
     4141 cgaaaacgcg atgaaggcgg gactgactaa gtcgccgcgc gtacatgacc tgcggcacac
     4201 atgcgcgtcg tggatgatcc aggctggcgt cccgctgccc gtcatccagc agcatctggg
     4261 ccacgagtcc attcagacca ccatcggggt ctacgggcac ctcgaccgac gcagcgccca
     4321 agtggccgct gacgctatcg gctcggcgtt gagctgaccg gaacggctgc gttctgcagt
     4381 tgctgtattc ctcggctagg tggagcgtct gcgccggatt tcttcggcgc caaggcttcc
     4441 cagcgtcttg agtagtcggc gaatggcatc tgggacgtct tcgtgggggc ggatcaacgg
     4501 cagaaggtca gccagccgcg gaggcggttg acgtatgtgc gagcgctcgg cgctgatttg
     4561 gggagcgcgt gagcgaatac cgcgagtgtt gtgcagccgg ggagtgctgc tgccggagcc
     4621 aggacagcgt cgaatccggc gtctcgggcc gcggtggcat ggttcgggta accgtgcagt
     4681 cgtcggcggt gaggtcatcg gcatcgacgc cgagatgtgc tcgcgtggtg gggtcggtga
     4741 ggtcgaggac gtccaggctg accgataccc ggcccacacg gcggcgcact tcgaacgggt
     4801 cgacaccgtc gtcgaggaag tggcgaaaaa actctgccca ggcgccttgc tcgcggttgg
     4861 aggcgtacca gacgcccggt tcgctagttc ggtgatagcg accggggccg cgagcgggat
     4921 ctgcgcacga cgtcagtgga tggcgggtcg ggccctggtg ccagtagctg ccggtcagct
     4981 tgcgtcgcgg tgcggtggcg accgcgtcag cggggctcaa acgtaggaac cgtcgataaa
     5041 ggattccgcc gcatgccgga ctcgctcgta gtggtcctcg gcgagcagct cgaccggtcg
     5101 cgcgccgtca aggatccggt ttttggcgtg cagccattgg ccgacgccgc ggggggtgag
     5161 cgagtcagac aacagaagga cgaggtcgcg aagctgggcg agtcggtcat agcggtctgg
     5221 gcggatgtcg ccggtgcgcc agccgcggac cgcgcggtcg gagacttgcg taaccgcggc
     5281 aacgtcgagc tgtgtgacgc cgaagggttt cagtgcgtcc acgatctcgc tggcctcgac
     5341 ggcccgatcc aatgcagcgg ccatcccaaa acctccagca cttccggttt cactaccgct
     5401 tatgctaccg gtgcgtggcg gtgggtgaca acgacctcgc gttagcgcgg tgggcgatcg
     5461 gactgaagtc aaccgaacgg ggagagccga cgggtttcat gcgctgcggt cgcgtaagcg
     5521 tcgtgcgtat cggtcatggc ccgcggcggc atgttcaaca actctgcacc gacctgccgt
     5581 ggagccaacg cctcaccgaa gtctggcgca tgaccgaccc ccacgagcgc gacgcctccc
     5641 tcgcactacg ctctgcacac gggcatcgtc tgcgcaccgc ggtcaagtgg taccgcaccc
     5701 agggccgcct gcacaccgga gatccgatcg ccatggccgc cgacgcgctg aacgcctacc
     5761 tccacgaccg tgcccgtcac aaggacgccc tgttggtgtg tgacacctgg ga
//
LOCUS       DASRJA010000211         4006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2401889,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000211 DASRJA010000000
VERSION     DASRJA010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2401889"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFC01_09425"
     CDS             <1..183
                     /locus_tag="VFC01_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HZN79890.1"
                     /translation="YSGVYSSFLKHAIRQSERYGVPAHQLLHRAGQRKLIGGQEDQLI
                     DIALEIKREQDTAAAK"
     gene            203..382
                     /locus_tag="VFC01_09430"
                     /pseudo
     CDS             203..382
                     /locus_tag="VFC01_09430"
                     /inference="COORDINATES: protein motif:HMM:NF027839.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemophore-related protein"
     gene            481..864
                     /locus_tag="VFC01_09435"
     CDS             481..864
                     /locus_tag="VFC01_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemophore-related protein"
                     /protein_id="HZN79891.1"
                     /translation="MSATTVRRGLYGMFAGGLLAFGSAAIVMPVANAVPDSCSQSGIA
                     TTASSVSASTAAYLAAHPQTNEELTDIARQPADQAEQAYQVYFDRNPQIANELQAINQ
                     PASDLLTQCGVNVTPTPISEILQTL"
     gene            891..1883
                     /locus_tag="VFC01_09440"
     CDS             891..1883
                     /locus_tag="VFC01_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016888943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZN79892.1"
                     /translation="MTAAPTERRRRIGSMEVLLASLIALALSAGAVSAFVAGNAEVSV
                     AATVFCGVFVQALPFLVLGVVISGLIAAFVSADRLARWLPRRPAMAVAVAGVGGAALP
                     GCECGSVPVARRLFGEGVSGAAALTFMLSAPAINPVVLVATAVAFPGQPAMVAARCVA
                     SLITAVTMGLIWLRWGRAEWVTRRLPATHDTDAPRWTMFTEAARHDFLQAASYLVLGA
                     AAAAVLRVALPPWVFQHLAGHLVLGVLTMALFAVVLALCSEADAFVAASLTMLPLLPR
                     LVFLVVGPAVDVKLSAMQAGLFGRPFAIRFGPMTFVVATVVATIVGLVILGGAR"
     gene            1880..2584
                     /locus_tag="VFC01_09445"
     CDS             1880..2584
                     /locus_tag="VFC01_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HZN79893.1"
                     /translation="MSRGTQNTLVLLVGLSALMMVIKGTYLHFVKPSLLPWLIAAAVV
                     LIALALAAIVRDLRREPVDDDGHRHRPLLMWLLLMPIALTTFVVPPPLGAQGAAPEAV
                     AATEPQKRAFPPLPTGRAPTVSIPDLVMRAAADSTNSLGGRLITVTGFTLRYPEGTDL
                     GRVVIVCCAADAQLARIHLSGPAAAEAADYPEDTWLQIEGTVVPGTSVAATNFIPTLA
                     VSRVTRIDKPANTYAY"
     gene            complement(2581..3483)
                     /locus_tag="VFC01_09450"
     CDS             complement(2581..3483)
                     /locus_tag="VFC01_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79894.1"
                     /translation="MSTPESAGRASPPTERVVRILDFLAARPEERFGLSDLARKLGLS
                     KPTCLGIVTSLTDAGYLVRDAGDKTYRLGPSLITLGHKAQESMRVSPAAREELRRLSA
                     RFGVTAALSGVIDDRITLLDLVAPAGAGPGVEVGQSYPFAPPVGLMFVLWDDEAARDW
                     LAKEPTIPLRTNTDRLNRVIAACRADGYLVERLTPGGRRLYSLMAGMSSKLPDELRAL
                     LGELVSDIGERVYLRGENGGRRRHDISVISAPVYDHYQRQVMVASMHIGKALTDNEIT
                     ERARALVATADAVTKQLGGVKPAR"
     gene            3570..>4006
                     /locus_tag="VFC01_09455"
     CDS             3570..>4006
                     /locus_tag="VFC01_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79895.1"
                     /translation="MYSQPLMEAIAEAEKLVGEAPHIESEADLLEGLQYLAGCIAACT
                     HVAFDYERDHPFLHSGTGPFTKMGLDNPDTLYFGTRVLPEHDYVVTGKRGTTTDVSFQ
                     LLGGEYSDKVVPDSETAFDDRKLDIAADGSFEWRFRPETPSQLV"
BASE COUNT          610 a         1364 c         1332 g          700 t
ORIGIN      
        1 tactccgggg tgtactccag cttcctcaaa cacgccatcc gccaatccga gcgatacggc
       61 gtgcccgccc accagctgct gcaccgggcg ggccagcgca agctgatcgg cggccaggaa
      121 gaccagctca tcgacatcgc attggagatc aagcgggagc aggacacggc ggccgcgaag
      181 taacgcttca gggcagtcgg cgaataccag agtggacagt gaggcgcgtg gcacggtcgg
      241 acagttcgac ggctacttca acgaccaccc gcaggaggcc gacgaactgc gcgcgatcca
      301 gcagcccctg gtcgactaca agaaccactg cggcatggag gtgtcgccga ccgacgctct
      361 gaccgtgctt gccgaggtct gacacgccgc ctgcgctgcg ggttgtttag gcccccccgc
      421 gtaccggata cgcctgttgg tcgcgagtca atcgcagacc gaaggagtga ttcgatgcat
      481 atgtccgcaa cgaccgtgcg acgcggcctt tacggaatgt tcgccggggg tctgctggct
      541 tttggatcgg cggccatcgt catgccggtc gccaacgcag tcccggactc ttgtagccaa
      601 agcggcatcg ccaccaccgc aagctcggtt tccgcgagca cagcggcgta cctggcggcg
      661 cacccgcaga ccaacgagga gctgacggac atcgcaaggc agcctgcgga ccaggctgag
      721 caggcctacc aggtgtactt cgacagaaac ccgcagatcg ccaacgagct gcaggcgatc
      781 aaccagcctg ccagtgatct gttgactcag tgcggcgtca acgtgacacc gacgccgatc
      841 tcggagattc tgcagacgct ctaatcaggt agcgcaggca agatggggcg gtgaccgccg
      901 ccccgaccga acgccgacgg cgtatcgggt cgatggaggt cttgctcgct tcgctcatcg
      961 cgctggcctt atcggccggc gcggtgagtg cttttgtggc cggtaacgcc gaggtgagcg
     1021 tggccgcgac ggtgttctgc ggcgtcttcg ttcaggcgtt gccctttctg gtgctcggcg
     1081 tcgtcatcag cgggttgatc gcggccttcg tttcggccga ccggctcgcg cgctggctgc
     1141 cgcgtcgccc tgcgatggcc gtcgcggtcg ccggggtggg cggtgcggcg ttgccaggct
     1201 gcgaatgcgg ctcggtgccc gtcgcgcggc gactgttcgg cgagggagtc tccggcgccg
     1261 ccgcactgac gttcatgctg tccgcgcccg ccatcaaccc ggtcgtgctg gtggccacgg
     1321 cagtcgcctt cccgggtcag ccggcgatgg tggcggcccg ctgcgtggcc tcgttgatca
     1381 ccgcggtcac gatgggactg atctggttgc gttggggccg cgccgagtgg gtgactcgac
     1441 gcctgccggc gacgcacgac accgacgcgc cgcgctggac gatgttcacc gaagccgccc
     1501 gtcacgactt cctgcaggcc gcctcctatc tggtgctcgg cgccgcggcc gccgccgtat
     1561 tgcgagttgc cttgccgccg tgggtttttc agcacctcgc gggacacctt gtcctcggcg
     1621 tcctcaccat ggccctgttc gcggtcgtgc tcgcgttgtg ttcggaggcg gacgcgtttg
     1681 tcgccgccag cctgacgatg ctgccgctgt tgccgcgact ggtgtttctg gtcgttgggc
     1741 cggcggtcga cgtcaagctg tcggcgatgc aggcggggct gttcggccgc ccgttcgcaa
     1801 tccgtttcgg cccaatgacg ttcgtcgtcg ccaccgtcgt cgcgaccatc gtcggcctgg
     1861 tgatcctcgg cggtgcacgg tgagccgggg cacccagaac accctcgtgc tcctcgtcgg
     1921 gttgagcgcc ctcatgatgg tgatcaaggg cacctacttg cacttcgtga agccgtcgct
     1981 gctgccgtgg ctgatcgccg ccgccgtcgt gttgatcgcc ctcgcgcttg ccgcgatcgt
     2041 tcgcgacctg cgccgcgagc ccgttgatga cgacgggcac cggcatcggc ccttgctgat
     2101 gtggctgctg ctgatgccga tagcgctgac gacgttcgtc gtgccgccgc cgctgggcgc
     2161 gcagggtgcg gcgccggaag ccgtagcagc gacagaaccg caaaagcggg cgtttccgcc
     2221 gctgcctacg gggcgggcgc cgacggtgtc gattcccgac ctagtgatgc gcgcggccgc
     2281 cgactccacc aacagccttg gcggacggct gattacggtc accgggttca cgctgcgcta
     2341 tcccgagggc accgatctgg gccgcgtcgt catcgtctgc tgcgcggccg acgcgcaact
     2401 ggctcgtatc catctgtccg gtccggccgc ggcggaggcg gccgactacc cggaggacac
     2461 ctggctgcag attgagggca ccgtcgttcc cggaacatcc gtggcggcga cgaacttcat
     2521 cccgacgctg gcggtgtcac gggtgacgcg catcgacaag ccggcgaaca cctacgcgta
     2581 ttagcgcgcc ggtttcacgc cacccagctg tttggtgacc gcgtcggccg tcgccaccaa
     2641 tgcccgcgcc cgctcggtga tctcgttgtc ggtcaatgcc tttccgatat gcatggacgc
     2701 gaccatcacc tggcgttggt agtggtcgta gaccggcgcg gaaatcacgc tgatgtcatg
     2761 gcgcctgcgc ccgccattct cgccacgcag gtacacccgc tcgccgatgt ccgacaccag
     2821 ctcgcccagc agcgcccgca gttcgtcggg cagcttgctc gacatccccg ccatcaacga
     2881 atacagccgt cggccgcccg gcgtcaggcg ctcgacgaga tagccgtcgg cgcggcatgc
     2941 cgcgatcacc cggttgagcc ggtcggtgtt cgtccgcagc gggatcgtcg gctccttcgc
     3001 gagccagtcg cgcgcggcct cgtcgtccca cagcacgaac atcagcccga ccggcggcgc
     3061 gaacgggtaa ctctgaccga cctccacgcc cggcccagcc cctgcgggcg cgaccaggtc
     3121 cagcagcgtg atccggtcat cgatcacccc cgacagcgcc gccgtcacgc cgaaccgcgc
     3181 cgacaaccgg cgcagctcct cccgggctgc cgggctgact cgcatcgatt cctgcgcctt
     3241 gtgcccgagc gtgatcaacg acgggcccag tcggtacgtc ttgtccccgg cgtcgcgcac
     3301 caggtatccg gcgtcggtga gcgacgtgac gatgccaagg caggtcggct tgctcagccc
     3361 cagcttgcgg gcgaggtcgg acagcccgaa gcgttcctct ggacgggcgg cgagaaaatc
     3421 caggatgcgg accactcgtt cggtgggcgg cgacgcgcgg ccggcggact ctggggtgga
     3481 cacgagagca gggtaccctg ctgttccata tatgtaccgc tgcggtcgat atattgactc
     3541 ttggtagaac gcgttctagt ttcgagggcg tgtactcgca gccactgatg gaggcgatcg
     3601 ccgaggccga gaagctggtc ggcgaggcgc cccacatcga atccgaagcc gatctgttgg
     3661 aaggcctgca atacctcgca ggctgcatcg cggcgtgtac gcacgtggcg ttcgactacg
     3721 agcgtgacca tccgttcctg cacagcggga cgggcccgtt caccaagatg ggcctcgaca
     3781 atcccgacac cctctacttc ggtacccgtg tgctgcccga gcacgactac gtggtgaccg
     3841 ggaagcgtgg caccaccacc gacgtgagct tccagctgct cggcggtgag tactcggaca
     3901 aggtggtgcc cgacagcgag acagcgttcg acgatcgaaa gctcgacatc gccgcggatg
     3961 gcagcttcga gtggcggttc cggccggaaa cgccgtccca gctggt
//
LOCUS       DASRJA010000212         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1272027,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000212 DASRJA010000000
VERSION     DASRJA010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272027"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VFC01_09460"
     CDS             <1..754
                     /locus_tag="VFC01_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN79896.1"
                     /translation="VYREPDKVRRSMGLTGQVATVDELLTGRENIRMIGGLYGIRRKD
                     LAQLGDQLLEQFSIADAADRVVKSYSGGMRRRLDLAVSLLAAPPVLFLDEPTTGLDPR
                     SRSDLWDVLRGLVQGGTTLLLTTQYLEEADQLADNIVVIDRGRIIAEGSPLQLKQQAG
                     NASLIVTVSKAEDLSAAKALMNKTGAEVFVDAGARQLTAAAEGLPDMVRVAGWLRDSD
                     IDVDDIGLSRPSLDDVFLSLTGHRTAEEEVGA"
     gene            751..1599
                     /locus_tag="VFC01_09465"
     CDS             751..1599
                     /locus_tag="VFC01_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN79897.1"
                     /translation="MTAVESPPRIQLQSVRTHPTNLVQQSWIMVKRNMIHTKRMPEML
                     SDVTVQPIMFVLLFAFVFGASIATGGGASYREFLLPGIQAQTIVFTAFVVASGITADV
                     EKGIIDRFRSLPISRSSVLIGRSVASLLHSSIGVVVMAVTGLAIGWRIRGSVAEAALA
                     FALILVFGFAMIWFGILIGSVMRSVEAVNGVMFTVLFPITFLANTFAPTEPMQHWLRV
                     IAEWNPVSSLAQAMRELWGNGGPAPASAQLPLHHPILATILWSLALTAVFAPFALRAY
                     ARRTGG"
     gene            1878..2756
                     /locus_tag="VFC01_09470"
     CDS             1878..2756
                     /locus_tag="VFC01_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HZN79898.1"
                     /translation="MGKFAAVIEDGSFDAAAARLHVTPSAVSQRIKALEQRVGQVLVV
                     REKPCRATAAGAPLIRLAAQTALLEIEALAEMGGGDGSLPRIALAVNADSMATWFIGV
                     LEALDDVLFDIRIEDQDHSTRLLREGVVMGAVTTERHAVAGCRVGPLGVMRYVPVASP
                     SYVKRYLADGFTARAVEAAPSLAWNRDDGLQDMLVRRVFRRDIARPQHFIPTVEGFGA
                     AVHNGLGWGMFPTSLAAPRLKDRSFVRVSDEHLDVPLYWQCWKLDSPIVGRITAAVKS
                     AAAALRSQKPLGRRHG"
     gene            complement(2886..3734)
                     /locus_tag="VFC01_09475"
     CDS             complement(2886..3734)
                     /locus_tag="VFC01_09475"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN79899.1"
                     /translation="MDVTQPTNDEQNKHWNTTAGCAWVDAQDLLDQMMKGLEDLLVDG
                     VAAGSGRRVLDVGCGTGGTTLAVARRLGTEGQCIGADVSEPMIAAARARAERDGIPAS
                     FILADVQTHPFERASVDTIISRFGVMFFNDPVQAFANLRRAATDDGALHVIAWRSAAE
                     NPFMTTGLREAVPLLPNLPDRPADGPGQFSFADPQRVAAILDAGGWADVDLQPIDVEC
                     TLPEPELIGFITRLGPVGLALQGEDERTRAQVIEAVRPAFDPFVHGTEVRFTAACWMV
                     SAQASG"
BASE COUNT          612 a         1160 c         1326 g          687 t
ORIGIN      
        1 cgtctaccgc gaacccgaca aggtgcgccg cagcatgggt ctcaccggcc aggtcgccac
       61 cgtcgacgaa ttgctcaccg gccgagagaa catccggatg atcggcggcc tgtacggcat
      121 ccggcgcaaa gacctcgcgc agctgggaga tcaactgctg gaacagtttt cgatcgccga
      181 cgccgccgac cgggtggtca agtcgtactc cggcggcatg cgccgtcggc tcgacctcgc
      241 cgtcagcctg ttggcggcgc cgccggtgct gtttctcgac gagcccacca ccgggctgga
      301 cccgcgcagt cgcagcgact tgtgggacgt gctgcgcggc ctcgtccagg gcggcaccac
      361 gctgctgctt accacgcagt atttggagga agccgatcag ctcgccgaca acatcgtggt
      421 gatcgacaga ggccgaatca tcgccgaggg ctcgccgctg cagctcaaac agcaggcggg
      481 caatgccagc ctgatcgtca ccgtcagcaa ggccgaggac ctgtccgccg cgaaggccct
      541 gatgaacaag acgggggctg aggtgttcgt cgacgccggt gctcgtcagc tcaccgcggc
      601 cgccgagggg ctgcccgaca tggtccgggt ggcgggttgg ctacgcgaca gcgatatcga
      661 tgtcgacgac atcggcctgt caaggccaag cctcgacgac gtcttcctgt cgctgaccgg
      721 acatcgcacc gcagaggagg aggtaggggc atgacggccg tcgaatcacc tccgcgcatt
      781 cagctgcagt ccgtgcgcac ccacccgacc aatctagtgc agcagtcgtg gatcatggtg
      841 aagcgcaaca tgattcacac caagcgcatg cccgagatgc tgagcgacgt gacggtgcag
      901 ccgatcatgt tcgtgctgct gttcgcattc gtcttcggtg cgtcgatcgc gacgggcggc
      961 ggcgcctcct accgggagtt cctactgccg ggcatccagg cgcagacgat cgtcttcacg
     1021 gcgttcgtcg tagcgtcggg gattaccgcc gacgtcgaga agggcatcat cgatcgcttc
     1081 cggtcactgc cgatttcccg atcgtcggtg ctgatcggcc gcagcgtggc cagcctgctg
     1141 cattcgtcga tcggagtggt ggtgatggcg gtgaccgggc tggcgatcgg atggcggatc
     1201 cggggcagcg tcgccgaggc tgcgctggcg ttcgcgctga tcctggtgtt cggcttcgcg
     1261 atgatctggt tcggcattct catcgggtcg gtgatgcgat cggtcgaggc ggtcaacggt
     1321 gtgatgttca cggtgctgtt cccaatcacg ttcctggcca acacgttcgc accgactgag
     1381 ccgatgcagc actggctgcg ggttatcgcc gaatggaatc ctgtgtcatc gctggcgcag
     1441 gcgatgcggg agctgtgggg caacggtggg cctgcgccgg caagcgccca actgccgctg
     1501 catcacccga tcctggcgac catcctgtgg tcgctggcgc tgacggccgt cttcgcgccg
     1561 ttcgcgctgc gcgcgtacgc gcgtcgcacc ggaggttgag tcagcgccgc ggccttaagc
     1621 cgatcggcgc gtagcgatcg tcccgatcca gcgttggcgc tgtgcctgat aatccgcgat
     1681 cgcttcggcg atggaatggc ccggtagtgc gcgatctggt ctcaagcggt tcgcagagcc
     1741 cagccctcgt cggctgcgtc acggacacag tggcgcatgc cgccgcggag ctacgccgag
     1801 ttggtgaata tgtagagctg atgattcttc ccacgcatta gtagtgctaa tgcggagacc
     1861 tgacgtaagg cgacgttatc ggcaaattcg ccgctgtgat cgaagacggc tcgttcgatg
     1921 cggcggcggc gcgactgcat gtcacgccgt cggcggtgag ccagcggatc aaggctctcg
     1981 aacagcgggt ggggcaggtg ctggtggtgc gggaaaagcc ttgtagagca acggcagcgg
     2041 gtgctccgtt gatacggctg gcagctcaaa ctgcgctgct ggagatcgag gcactggccg
     2101 agatgggcgg cggggatggg tcgttgccgc gaattgcgtt ggctgtcaat gccgattcga
     2161 tggcgacgtg gttcatcggg gtgttggagg cgctcgatga tgttctgttc gatatccgga
     2221 tcgaggatca ggatcactcg acgcggttgt tgcgtgaggg ggtggtgatg ggcgcggtca
     2281 ccaccgagcg ccacgcggtg gccggttgtc gggtggggcc gctgggcgtg atgcgctatg
     2341 tgcccgtggc cagtccgagt tacgtcaagc ggtacttggc cgatgggttc acagcgcgag
     2401 cggtggaagc ggcaccgtct ctggcgtgga atcgtgatga tgggctgcag gacatgttgg
     2461 tacgcagagt atttcgccga gacatcgccc gaccgcagca cttcatcccg acagtggagg
     2521 gattcggggc ggcggtgcac aacggactgg ggtggggcat gttcccgact agcctcgcgg
     2581 cgccgcggct aaaggacagg tcattcgtgc gtgtgtccga cgaacacctc gacgtgccgt
     2641 tgtactggca gtgctggaaa ctcgacagcc cgatcgtcgg ccgcatcacc gccgcggtga
     2701 agtcagcagc agcggctctg cggagccaga agccgctggg ccgccgccac ggctaacgat
     2761 tacgtaggag ccttacttcg acgtcgcccg ccgggtcgac cattgcgtgc tgctgagcgg
     2821 cggccgagtg gtgtcgtcgg gcgcctcggc cgacgtgctc accgaggcca acgtggcgtc
     2881 ggcggttatc ccgacgcttg ggcgctgacc atccagcacg cggcggtgaa ccgaacctcg
     2941 gttccgtgca caaagggatc aaaggcggga cggacggcct cgatgacttg ggcgcgggtc
     3001 cgctcgtcct cgccttgaag tgccagaccg acaggaccca gccgcgtgat gaagccgatc
     3061 aactccggct cgggcagggt gcactcgacg tcgatcggct ggaggtcgac gtcggcccag
     3121 ccacccgcgt cgaggatcgc agcgacccgt tgcgggtcgg cgaaggagaa ctgtcccggg
     3181 ccgtctgccg ggcggtcggg aaggttcggc agcagcggga cagcctcacg caggccggtc
     3241 gtcatgaacg gattctccgc ggcgctgcgc caggcgatga cgtgaagcgc gccgtcgtcg
     3301 gttgcggcgc gccgcaagtt cgcgaaggcc tggaccgggt cgttgaagaa catgacgccg
     3361 aatcgcgaga tgatcgtgtc gacgcttgcg cgctcaaacg gatgggtctg cacgtcggcg
     3421 aggatgaaac ttgctggtat gccgtcccgt tcggccctgg cgcgggcggc ggcgatcatc
     3481 ggctcggaca cgtcggcgcc gatgcactgg ccctcggtgc cgagcctgcg cgcgacggcg
     3541 agtgtggtgc cgccggtacc gcagccgacg tcaagcacac gccgccccga tccggcagcc
     3601 acgccgtcga cgagcaggtc ttccaatccc ttcatcatct ggtccagcag gtcctgcgcg
     3661 tcgacccagg cgcatccagc tgttgtgttc cagtgcttgt tctgctcgtc gttggtcggt
     3721 tgggtgacat ccatgggccg attatgccga tccaagtcaa cttgaagtcg acccggcgat
     3781 tgggc
//
LOCUS       DASRJA010000213         6484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2401807,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000213 DASRJA010000000
VERSION     DASRJA010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2401807"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VFC01_09480"
     CDS             complement(<1..994)
                     /locus_tag="VFC01_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010949543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HZN79900.1"
                     /translation="MADDAPTQDLSEETQPSEDQSETLTVDSLAGALGTTSERVLEAL
                     AEFDGKPRDPQSAVEADEADRVRQALTESTEPGGDEPSADDGDEPESRLILETAPELE
                     RADYLPLFMAPQPVQLDYDSGADDEDEDEDGGTDSDEDQGERPAGRRRRRGRRGRGRG
                     RGEQGGDDSDDGDSGDAQTDGKGGDEDSGDESDEDSGDEESGSEGANRRRRRRRRRKS
                     GSGDDNDDSGSPDDPPNTVVHERAPRSKDKDKDNGEIQGISGSTRLEAKRQRRRDGRD
                     AGRRRPPILSEAEFLARREAVERTMVVRDKVRTEPPHEGARYTQIAVMEDGVVVE"
     gene            complement(1290..1700)
                     /gene="ndk"
                     /locus_tag="VFC01_09485"
     CDS             complement(1290..1700)
                     /gene="ndk"
                     /locus_tag="VFC01_09485"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZN79901.1"
                     /translation="MTEQTLVLIKPDGVERRLIGEILSRIEGKGLTIAALELKTVDDD
                     LARRHYAEHEGKPFFGSLLEFITSGPVVAAIVEGARAVAAFRQIAGATDPVEKAAPGT
                     IRGDLALITQDNLVHGSDSPESAVREIELWFPGR"
     gene            1809..2249
                     /locus_tag="VFC01_09490"
     CDS             1809..2249
                     /locus_tag="VFC01_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005256792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HZN79902.1"
                     /translation="MASPQTRQRLAPVQIAAYVVGVAFLLPGVLGFIPGITTNYEMLD
                     FAGHHSGAKLLGIFGISVLHNIVHLAFGIAGLVFARTFTGARRYLIGSGVIYLLLSIY
                     GLLIDHESAANFVPIDSADNWLHLVLAVGMLALGFVLGRRRSAA"
     gene            complement(2246..2647)
                     /locus_tag="VFC01_09495"
     CDS             complement(2246..2647)
                     /locus_tag="VFC01_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HZN79903.1"
                     /translation="MSPEQPQPADQPDPWKSFRGVMAGTLILEAIVVLLALPVVAVAD
                     HGLTPLTAGYLIGFALVLVLLAGVQGRPWAIWVNLGIQVVLIAGWVVHGAVGFVGVIF
                     TAVWLLIAYLRAEVLRRQKHGLLPGQQQPPG"
     gene            complement(2761..4170)
                     /locus_tag="VFC01_09500"
     CDS             complement(2761..4170)
                     /locus_tag="VFC01_09500"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HZN79904.1"
                     /translation="MTEPVPTPDEIASLLQVEHLLDQRWPETKLEPSTARISALMEML
                     GSPQRGYPSIHIAGTNGKTSVARMVDALLTAFNRRAGRTTSPHLQSAVERISIDGRPI
                     SPAQYVATYREIEPFVQLVDQQSEATGGPAMSKFEVVTGMAFAAFADAPVDVAVIEVG
                     LGGRWDATNVVNAPVAVITPIGVDHADYLGDTIAEIAGEKAGIITKQDDDLVPTDTVA
                     VIGRQVPEAMEVLLAQAVRADAAVAREDSEFAVLGRQIAVGGQLLELQGLGGVYSDIF
                     LPLHGEHQAHNAVVALAAVEAFFGAGAERQLDIDTVRAGFAAATSPGRLERMRTAPTV
                     FIDAAHNPAGATALAQALQEEFDFRFLVGVVSVMADKDVDGILAALEPAFDQIVVTHN
                     GSPRALEVEALALRAEERFGPERVVTAATLPDAIETATALVEESGSEGEGFSGAGVVI
                     TGSVVTAGAARTLFGRDPE"
     gene            complement(4167..>6484)
                     /locus_tag="VFC01_09505"
     CDS             complement(4167..>6484)
                     /locus_tag="VFC01_09505"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZN79905.1"
                     /translation="EDFGRELFIDKVWDWKHDSGGSIAAQMRRLGDGVDWSRDRFTMD
                     DGLSRAVRTIFKRLFDSGLIYRAERLVNWSPVLETAVSDLEVKYEDVEGELVSFRYGS
                     LDDSEPHIVVATTRVETMLGDTAIAVHPDDERYRKLVGTTLPHPFLDRELIIVADDHV
                     DPEFGTGAVKVTPAHDPNDFEIGLRHQLPMPSILDTKARIIDTGTEFDGLDRFEARVK
                     VREALAEQGRIVAEKRPYLHSVGHSERSGEVIEPRLSMQWWVKVESLAKAAGDAVRNG
                     DTVIHPPSLEPRWFGWVDNMHDWCISRQLWWGHRIPIWHGPDGETVCVGPDETPPEGW
                     EQDPDVLDTWFSSALWPFSTLGWPDHTDDLAKFYPTTVLVTGYDILFFWVARMMMFGT
                     YVADDSAITLDGKRGPQVPFENVFLHGLIRDEFGRKMSKSRGNGIDPLDWVEQFGADA
                     LRFTLARGASPGGDLSIGEDHARASRNFATKLFNATRFALMNGAAPAPLPGISELTDA
                     DRWILGRLEEVRAEVDSAFDSYEFSRACEALYHFAWDEFCDWYVELAKVQLAEAVQHT
                     TAVLAAVLDTLLKLLHPVMPFVTEVLWKNLTGGESLVIADWPQPSGFTLDPVAAQRVA
                     DMQKLITEVRRFRSDQGLNDRQRVPARLSDIEHADLDAQIPAVRSLAWLTEGDDSFSP
                     SAHVEVRLSRGPVVVEVDTSGTVDVAAERRRLEKDLAAAEKELAGTAGKLGNDAFLAK
                     APADVVEKIRARQQVAREEVDRITARLASLK"
BASE COUNT          992 a         2272 c         2068 g         1152 t
ORIGIN      
        1 gttcgacgac cacgccgtct tccatcaccg cgatctgcgt gtagcgcgcc ccctcgtgcg
       61 gcggctcggt gcggaccttg tcgcgcacga ccatcgtccg ctcgacggcc tcgcgccggg
      121 ccaggaactc ggcctcgctc aggatcggtg gacggcggcg gcccgcgtcg cggccgtccc
      181 ggcggcgttg ccgcttggcc tccagccggg tcgatccgct gatgccctgg atctcgccgt
      241 tgtccttgtc tttgtccttg ctgcgcggcg cgcgctcatg cacgacggtg ttcggtggat
      301 cgtccggtga gccgctgtcg tcgttgtcgt cacccgagcc ggacttgcgg cgccgacgtc
      361 gacggcgacg acggttcgcc ccctcgctgc cgctctcctc gtcgccgctg tcctcgtcgg
      421 actcgtcgcc ggaatcctcg tcgccaccct tgccatcggt ctgcgcgtcg ccggagtcac
      481 cgtcgtcgga gtcgtcgccg ccctgctcgc cgcgaccacg gccgcgacca cgccgccccc
      541 tgcggcggcg cctgcctgcg ggtcgctccc cttggtcctc gtcggaatca gttccgccgt
      601 cctcgtcctc gtcctcgtcg tcagcgccgc tgtcgtagtc cagctggacc ggctgcggcg
      661 ccatgaacag gggcaggtag tcggcgcgct ccagttcggg ggccgtctcg aggatcagcc
      721 gcgactccgg ctcgtcgccg tcgtcggcgg acggttcgtc gccacccggc tcggtgctct
      781 ccgtcagcgc ttggcggacg cggtctgcct cgtcggcctc gaccgcggac tgcgggtcgc
      841 ggggcttgcc atcaaactcg gccagcgctt caagcacacg ctcgctggtg gtgccaagtg
      901 caccggccag cgagtcgacc gtcagggtct ctgactggtc ctcactgggc tgagtctctt
      961 ctgataggtc ttgggtaggg gcatcgtcgg ccacgtattc tcctcaagcc cccgggcgcg
     1021 tcatgttttg acgcggccac gcgagggcta tcgctatggg cccgggttac ttctcccgag
     1081 cttgttatgg tctcgccccg aggggtccca gttcggaacc gtctcggtgc cggtctgaat
     1141 gatggctgga cggtcggcgc cgcaccgctg gggcgatggt cgcgctcgtc taagtcttca
     1201 ttcgggcatc cggcccgcgt aacaggtcgt ctacccgagg ccagtatccc acatcactcg
     1261 tcccagaccg accagcctgg aaacgccagc tatcggccgg gaaaccacag ctcgatctcg
     1321 cggaccgccg actccggcga atcggacccg tgcacgaggt tgtcctgagt gataagtgcc
     1381 aggtcaccgc ggattgtgcc gggggcggcc ttctccacgg ggtccgtggc gccggcgatc
     1441 tggcggaacg cggcaacggc ccgcgcgccc tccacgatgg ccgccacgac tgggcccgag
     1501 gtgatgaact ccagcaacga tccgaagaac ggcttgcctt cgtgttcggc atagtgccga
     1561 cgtgcgaggt cgtcgtcgac ggtcttgagt tcgagggccg cgatggtcag gcccttgcct
     1621 tcgatacggc tgaggatctc cccgatcagg cgccgttcga cgccgtcggg cttgatcaat
     1681 accagggtct gctctgtcac ggcggacagc ctagcggcac gtcacccatc caagagcggc
     1741 gcgggaacga atttcaggtg accggccgct cagtccgatg ccggcgaccg ctgaagaaag
     1801 gcataccgat ggcatcacca caaacccgtc agcgcttggc gcccgtgcag atcgcggcgt
     1861 acgtcgttgg cgtggcgttc ctgcttcccg gcgtgctggg tttcattccc ggcatcacga
     1921 cgaactacga gatgctcgac ttcgccggcc atcactccgg agcgaaactg cttggcattt
     1981 tcggcatttc ggtcctgcac aacatcgtgc acctggcatt cggcatcgct ggcctggtgt
     2041 tcgcgcgaac attcaccggc gcccggcggt acctgatcgg ctccggcgtg atctatctgc
     2101 tgttgtcgat ctacggattg ctcatcgatc acgagagcgc cgccaacttc gtcccgatcg
     2161 actccgccga caactggctg cacctcgtgc tggccgtcgg catgctcgca ctgggctttg
     2221 tgctgggccg ccgccgcagc gcggcctagc ccggaggttg ctgttgtccc ggcagaagcc
     2281 cgtgcttctg ccggcgcagc acctcggcgc gcaggtacgc gatcagcagc cacaccgcgg
     2341 tgaagatcac gccgacgaaa ccgacggcgc cgtgcaccac ccaccccgcg atcagcacca
     2401 cctggatccc gagattcacc cagatcgccc acggccggcc ctgcacccct gccagcagca
     2461 cgagcacgag tgcgaaaccg atcaagtaac ccgccgtcag cggcgtcaac ccgtggtccg
     2521 caacggcgac gaccggcagc gccaacagca ccacgatggc ttccaggatc agcgttcctg
     2581 ccatgacacc gcggaagctc ttccacggat ccggttgatc ggcgggctgc ggctgctctg
     2641 ggctcatgcc tcgactcccg ctcgctccgg tcctcgctcg ttcctcgctg cgatccccac
     2701 tcgcggtcgt cttcggggac tcgttcctcg ctgcgatcct cactcgcggt cgtcttcgga
     2761 tcattccggg tcccttccga acagcgtccg ggccgccccc gcggtgacga ccgaaccggt
     2821 gatcaccaca cccgcgccgc tgaatccctc gccctcgcta ccagactcct cgacgagcgc
     2881 cgtcgcggtc tcaatcgcgt cgggcagcgt cgccgccgtg accacccgct ccggcccgaa
     2941 ccgctcctcg gcgcgcagcg ccagtgcttc gacctcgagc gcacgcggtg atccgttgtg
     3001 ggtcaccaca atctggtcga aggccggctc gagcgcggcc agaatgccgt cgacgtcctt
     3061 gtcagccatc accgagacca cgccgaccag gaatcggaag tcgaactcct cctgcagggc
     3121 ctgcgcgagt gccgtggcgc cggccggatt gtgggccgcg tcgatgaaca ccgttggcgc
     3181 ggtgcgcatt cgctccagcc ggcccgggct ggtggcggcg gcgaaccctg cccggacggt
     3241 gtcgatgtcg agctgtcgct ccgcgcccgc gccaaagaac gcttcgaccg ccgccagcgc
     3301 gactaccgcg ttgtgcgcct gatgctcgcc gtgcagcggc aggaagatgt cggaatacac
     3361 gccaccgagc ccttgcagtt ccaacagctg gccaccgacg gcgatctgcc ggcccaggac
     3421 cgcgaactcg gaatcctcgc gtgccaccgc ggcgtcggcg cgaaccgcct gcgccagaag
     3481 cacttccatc gcctcgggta cctgacggcc gatgaccgcg acggtgtcgg tcggcaccag
     3541 gtcgtcatcc tgcttggtga tgatgccggc cttctcgccc gcgatttccg ctatggtgtc
     3601 gccgaggtag tccgcatgat cgacgccgat cggcgtgatc acggcgacag gcgcgttgac
     3661 gacattggtg gcgtcccaac gcccaccgag accgacctca atgaccgcca catccaccgg
     3721 ggcatcggcg aacgcagcga aagccattcc agtgaccact tcgaacttgc tcatcgccgg
     3781 cccgcctgtc gcttccgact gctggtcgac gagttggacg aacggctcga tctcgcggta
     3841 ggtcgcgaca tactgcgccg ggctgatcgg cctgccgtcg attgagattc gctccacggc
     3901 ggactgcagg tgcgggctgg tggtgcggcc ggcgcgacga ttgaacgcag ttaacagggc
     3961 atcgaccatg cgggcgaccg acgtcttgcc gttggtgcct gcgatgtgaa tcgacggata
     4021 gccccgctgc ggggagccga gcatctccat cagcgccgag atgcgggccg tactcggctc
     4081 cagtttcgtt tcgggccaac gctggtcaag caggtgctcg acctgtagca gtgaggcgat
     4141 ctcgtccggg gtggggacgg gttcggtcat ttgaggctgg ccagcctcgc cgtgatgcgg
     4201 tcgacctctt cgcgggccac ctgctggcgc gcgcggatct tctcgacgac atccgcgggc
     4261 gccttggcca agaacgcgtc gttgccgagc ttgcctgccg tgccggccaa ctccttttcg
     4321 gccgcggcta ggtccttctc gaggcggcgc cgttcggcgg cgacgtcgac ggtgcccgac
     4381 gtgtcgacct cgacgacgac cgggccccgc gacagccgca cctcgacgtg ggccgacggg
     4441 ctgaaggagt cgtcgccctc ggtgagccac gccagtgacc gcacagccgg gatctgcgcg
     4501 tcgaggtcgg cgtgctcgat gtccgacaac cgggcaggca cgcgctgcct gtcgttgaga
     4561 ccttggtcgc tgcggaagcg acggacctcg gtgatcagct tttgcatatc cgcaacgcgt
     4621 tgcgcggcaa caggatccag cgtgaagccg gacggctgcg gccagtcggc gatgaccagg
     4681 gactctccgc ccgtcaagtt cttccacaac acctcggtga cgaacggcat caccgggtgc
     4741 aacagcttga gcagcgtgtc gagcacggcg gccagcaccg ccgtcgtgtg ctgcaccgct
     4801 tctgccaact gcaccttggc gagctcgaca taccagtcgc agaactcatc ccacgcgaag
     4861 tgatacagcg cctcacacgc ccggctgaac tcgtagctgt cgaacgccga atccacctcg
     4921 gcgcgaacct cttccagccg gcccaggatc caccggtcgg cgtcggtcag ctcggaaatc
     4981 ccaggcagcg gtgctggcgc tgccccgttc atcagcgcga acctggttgc gttgaacagc
     5041 ttggtggcga agttgcgcga cgcacgggcg tggtcctcgc cgatggagag gtcgccgcca
     5101 gggcttgcgc cacgggccag ggtgaagcgc agcgcgtcgg cgccgaactg ctcgacccaa
     5161 tccagcggat cgatgccgtt gccgcgcgac ttactcatct tgcggccgaa ctcgtcgcga
     5221 atcagcccat gcaggaacac attctcgaac ggcacctgcg gaccacgctt gccgtcgagg
     5281 gtgatcgcgg aatcatcggc gacgtatgtg ccgaacatca tcattcgcgc gacccagaag
     5341 aacaggatgt cgtatccggt gaccaacacc gtggtcggat aaaacttcgc caggtcatcg
     5401 gtgtggtcgg gccaacccag cgtggaaaac ggccacaggg ccgacgagaa ccaggtgtcc
     5461 agcacatcgg ggtcctgctc ccagccctcg ggcggcgttt catccggccc gacgcatacg
     5521 gtttcgccgt cggggccgtg ccagatcggg atgcggtggc cccaccacag ctgacgcgag
     5581 atgcaccagt cgtgcatgtt gtcgacccaa ccgaaccacc gcggctcaag gctcggtggg
     5641 tgaatcaccg tgtcgccgtt gcgaactgcg tcacccgcgg ccttcgccag cgattcgacc
     5701 ttgacccacc actgcatcga aagccgtggc tcgatgactt caccgctgcg ctcggagtgt
     5761 ccaacgctgt gcaggtacgg acgcttttcc gcgacgatgc ggccctgctc tgccagcgct
     5821 tcgcggacct tgacgcgggc ctcgaagcgg tcgaggccgt cgaattctgt tccggtgtcg
     5881 atgatccggg ccttggtgtc caagatcgag ggcatcggca gttgatggcg caagccgatc
     5941 tcgaagtcgt tcgggtcgtg ggcgggcgtg actttgaccg cacccgttcc gaattcgggg
     6001 tcgacgtggt cgtcggcgac gatgatcagc tcacggtcga ggaacggatg cggcagcgtc
     6061 gtgccgacca gcttccggta gcgctcatcg tcggggtgca ccgcgatcgc ggtatcgccg
     6121 agcatcgtct cgacccgggt ggtagcgacc acgatgtgcg gttcgctgtc gtccagcgag
     6181 ccgtagcgga acgacacgag ctcgccttcg acgtcctcgt acttgacttc gagatcggag
     6241 acagcggtct ccagcaccgg cgaccagttc accagccgct cggcacggta gatcagcccg
     6301 gaatcgaaga gccgcttgaa gatggtgcga acggcccgcg acaggccgtc gtccatggtg
     6361 aatcggtcgc ggctccagtc gaccccgtcg cccaaccggc gcatctgcgc ggcgatgctg
     6421 ccaccggaat cgtgcttcca gtcccacacc ttgtcgatga acagctcgcg gccaaaatct
     6481 tcct
//
LOCUS       DASRJA010000214        15646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_145773,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000214 DASRJA010000000
VERSION     DASRJA010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15646
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145773"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFC01_09510"
     CDS             complement(<1..219)
                     /locus_tag="VFC01_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZN79906.1"
                     /translation="MRQCRGCGAAMSKRSQKVYCSNACQGSVRRDNSIKRWLESGEAR
                     IATYQDHYIRQYLADAQSSCCAICGGAST"
     gene            complement(277..351)
                     /locus_tag="VFC01_09515"
     tRNA            complement(277..351)
                     /locus_tag="VFC01_09515"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(317..319),aa:Thr,seq:tgt)
     gene            442..735
                     /locus_tag="VFC01_09520"
     CDS             442..735
                     /locus_tag="VFC01_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79907.1"
                     /translation="MSDFPNVYPGLARPDIERLLTLLDESASTEGRLSLSIATALNPL
                     VPDVARRIESYKQSGDVDEYVRMLRGATVLLLQKWVPNGRPPAPETISNSIEG"
     gene            complement(738..1439)
                     /locus_tag="VFC01_09525"
     CDS             complement(738..1439)
                     /locus_tag="VFC01_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79908.1"
                     /translation="MNLGKTLTDLATAPVRVGLAVADAGLDIANSAVGRARSGSGSVA
                     HMLGIDDAVERANRLARLMDEDQPLGRALAPGGPVDRLLQPDGLVDRLTAPGGVLDRL
                     TEENSGLMRAIAPGGLVDQVLAEDGLLDRLLAEDGVADRLLAEGGVIDKLTARNGPLE
                     QLATVADTLNRLAPGLEALEPTIEALREAVITLSQVVNPLSNIADRIPFPGRRPRGRP
                     TTRPVTSQRVIDADE"
     gene            1567..2286
                     /locus_tag="VFC01_09530"
     CDS             1567..2286
                     /locus_tag="VFC01_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN79909.1"
                     /translation="MAMAALPENLPEARILVVDDEANIVELLSVSLKFQGFEVHTASN
                     GPAALDKAREIRPDAVILDVMMPGMDGFGLLRRLRADGIDAPALFLTARDTLQDKIAG
                     LTLGGDDYVTKPFSLEEVVARLRVILRRAGRGVEEQRPSRLTFADIELDEDTHEVWKA
                     GEPVSLSPTEFTLLRYFIINAGTVLSKPKILDHVWRYDFGGDVNVVESYVSYLRRKVD
                     TGEKRLLHTLRGVGYVLREPR"
     gene            2317..3744
                     /locus_tag="VFC01_09535"
     CDS             2317..3744
                     /locus_tag="VFC01_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN79910.1"
                     /translation="MRGRGIPLRVGLVAATLVLVACGLLASGIAVTTILRHDLTNRVD
                     ETLLDASRSWAQAPRRMPAPPIEGPNPARPPSNFYVRGIDPDGRIWMAVNDRAAEPAL
                     PADNDVGPVPVTVGSIDNSNVQWRAMSVRGLRGELTTVAIDLSDVQTTVRALIYAQVG
                     IGAAVLLVLGVAGYWVVHRSLRPLTEVEQTAAAIAAGQLDRRVPERDARTEVGRLSLA
                     LNGMLAQIQRAVASSDSSAAAARTSEDRMRRFITDASHELRTPLTTIRGFAELYRQGA
                     ANDVEMLMSRIESESRRMGLLVEDLLLLARLDQQRPLERHRVDLLAIATDAVHDAQSI
                     APSERKIAMEVFDGPGTPEVLGDEARLRQVLGNLVANALQHTPDSAGITVRVGTDNDH
                     AVLEVCDEGPGMTKEDAHRVFERFYRADSSRTRASGGTGLGLSIVDSLVHAHDGTVSI
                     TTAPGQGCRFKVNLPRIADVPAHVS"
     gene            complement(3732..4136)
                     /locus_tag="VFC01_09540"
     CDS             complement(3732..4136)
                     /locus_tag="VFC01_09540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HZN79911.1"
                     /translation="MATVFTKIINGEIPGRFVYEDDDVVAFLTIAPITHGHTLVVPRA
                     EIDQWQDVDAPAFNKVMAVSQLIGKAVTKAFDVERAGVIIAGLEVPHLHVHVFPAHDL
                     SDFGFANADHNPSQESLDEAQAKIKEALAQLT"
     gene            4210..5421
                     /locus_tag="VFC01_09545"
     CDS             4210..5421
                     /locus_tag="VFC01_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HZN79912.1"
                     /translation="MTDLAAPRTAEVSERPTRSCVLDIVIPVYNEEDDLPGSVRRLHR
                     YLTAEVPYPSRITIADNASIDRTLAVAKELASELPDVDVIHLDRKGRGGALYTAWMAS
                     DAEVVAYMDVDLSTDLSALMPLVAPLISGHSDVAIGSRLAASSRVVRGPKREFVSRSY
                     NLILRGVLGAKFSDAQCGFKAVRADVARQLLPHVADTGWFFDTELLVIAERAGLRIHE
                     VPVDWVDDPDSRVDIVRTAINDLKGCWRVGRALASGALPVRELQRSLGREPLVPGVPR
                     GMVGQMVRFGIVGIASTVAFALLYLLLHPAMGAQAANLTALLLTAVANTAANRAFTFG
                     IRGRNGVARHQLNGLLVFAFCLAITSGSLFVLHRFDPAVGKVVELSVLVAANLVATLI
                     RFVALRRVFSA"
     gene            complement(5455..6336)
                     /locus_tag="VFC01_09550"
     CDS             complement(5455..6336)
                     /locus_tag="VFC01_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010840526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN79913.1"
                     /translation="MPCSSRRRARAISLAGRGAVVVGGSRGVGLAVAELLAAQGAGVV
                     INGRDADAATEAAQHITGAVAFSGSPADPAVADALIDTCIKEFGQIDVLVNCAGTAGL
                     ASESILKVTSAQFRELVDAHLGTTFETCRAAAPKMVGQGGGSIINTSSFAFLGDYGGT
                     GYPAGKGGVNGLTMALAAELKEHGVRANVVCPGAKTRLSTGSEFEAHMVELHRRGMLD
                     EASMQAALDAPPPESVAPTYAYLASDLAKDVTGQIFIAAGGFVGRFDRQTPSILGYRD
                     HQDSPPWSVDEIHAMIS"
     gene            complement(6299..6733)
                     /locus_tag="VFC01_09555"
     CDS             complement(6299..6733)
                     /locus_tag="VFC01_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN79914.1"
                     /translation="MSQETVEKTPVVIASESSWRCVQAGDKEGWLALMADDIVIEDPI
                     GDAVTNPGGGGVRGKDAVAAFFDTNIGPNQLTVTREETFPSSSPTEIAYILVLRTRFP
                     NGFTATVRGVFTYKVNDAGLITNLRGYWNMDAMQFAQEGEGN"
     gene            complement(6746..8743)
                     /locus_tag="VFC01_09560"
     CDS             complement(6746..8743)
                     /locus_tag="VFC01_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN79915.1"
                     /translation="MKHFSHLLQPGQIGSMTLRNRLVMSPMETMYGTSDGLPSERTRD
                     YFAARAKGGVGLITVGATGIDHRQPETPGGLHLGTDAAVDAHRALVEVVHEHGAKIQP
                     QIVHAGPDGLGPEMYGVTSIGPSVIPSYLTGRPSAEVTPEQLIEVFDLFKGAVRRAAE
                     AGYDGIELHAAHGYMFLGSFLAPQRNRRTDDYRGDSAKGRIRVVLEALAAIRSEIGDA
                     LPITLRISGYERVAGGRPIFETAQVAPQLVAAGVDAFHVSGGVIDRLVTGMVNSADDG
                     DALNVGAAAAVKQVVDVPVIAVGRIHDPERAEQILADGRADFIAMGRPMLADAELLTK
                     VATGHADRVRKCISCENCIDAMEQRFSVDCAVNPRTGKERELAAPRAPRPKKVVVIGS
                     GPAGMEAARVAAERGHQVTLFERTSQTGGALLWASILHPENEPFLRWLRDEVGRSSVK
                     VELGTEVSADDVVDLRPDAAVVANGGQVVLPTIPGSNLPHVQTGQALRERFGGWRQRL
                     VRPTAVRLASKAWMPLGHRVVIVGADLVALELAEFLAARGRLVTVLQADKTIAPELGN
                     KRKTEHMDRLDRLGVAVHVRAPVEQILPDSVVFTPYGGTSRQVAADSVVVAGTLQPNT
                     ELYEKLVAALPNAAVHAAGDCTGLGLIRKATEDGARAACAI"
     gene            complement(8740..9828)
                     /locus_tag="VFC01_09565"
     CDS             complement(8740..9828)
                     /locus_tag="VFC01_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN79916.1"
                     /translation="MSSPVRVFQVASGNVGTEMIRRIQNHPDLELAGLHCYSPDKVGR
                     DVGEIAGIGPVGVTATGTVKEILAARPDCVTFHGVWPDIELYTQVLEAGINIVTTADW
                     ITGHHRNTNHRRNGSTESEILQAACERGNSTLYGTGMNPGLAQILTIVHSADVADIES
                     VTCIESVDVSCHHSAETWQNCGFGRPVDDPEVPQLLELGTRVFEDGVRLMADCLDIEL
                     DPGPNGVKFSYQLGACTKDVDLGWYQLPAGSLGGCVLGYVGMVDDVKRVELRMDWQMT
                     PHTDPHWDIQGCYITKIDGDPCIYSKHMILPKPGTDFSSVESFAKIGMTVTGMPALNA
                     IRAVVDAAPGIVTSADLPLRAFAGRFAR"
     gene            complement(9835..10962)
                     /locus_tag="VFC01_09570"
     CDS             complement(9835..10962)
                     /locus_tag="VFC01_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HZN79917.1"
                     /translation="MKTKGALIWEFNQPWSIEEIEIGDPVKDEVKIQMEASGMCHSDH
                     HLVTGGIPMGGFPVLGGHEGAGIVTEVGPGVEDIAPGDHVVLSFIPSCGKCPSCQAGL
                     RNLCDLGAGLLNGAAVSDGTFRIQARGQNVFPMTLLGTFSPYMVVHRSSVVKIDPSIP
                     FEVACLVGCGVTTGYGSAVRTADIRPGQDVAIVGVGGVGMSALQGAVTAGARHIFVIE
                     PVEWKRDAALKFGATHVYPDINTALMGIAEVTAGFMAHKVIITVGELKGEDIDNYLMI
                     TAKGGTCVATAIGSLLDTQVTLNLAMLTLMQKNLQGTIFGGGNPHYDIPQLLSMYKAG
                     KLNIDDMITRQYKLEQINEGYQDMLDGKNIRGVIRYTDDDR"
     gene            complement(11022..11567)
                     /locus_tag="VFC01_09575"
     CDS             complement(11022..11567)
                     /locus_tag="VFC01_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN79918.1"
                     /translation="MPSLPREQLEDFVERWLEVNREAERKGDWRGLADFYTDDATYGW
                     NIGPKEDVMCVGIDEIRDIALGQEMEGLEGWRYPYQRVLIDDKIGEVVGFWKQVATDG
                     DGTEQEVYGIGGSWFRLNAEGKIEWQRDFFDFGHVSALYMDMIKAGKLSEGMQKRIER
                     GMAGEKVPGYYPLGKSPSPIW"
     gene            complement(11570..11776)
                     /locus_tag="VFC01_09580"
     CDS             complement(11570..11776)
                     /locus_tag="VFC01_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN79919.1"
                     /translation="MGYRVEVDLDLCQGHAMCELEAPDYFKVPKRGQVEILNDEPPEE
                     DRAQIEQAVWACPTQALSIREKED"
     gene            complement(11788..13134)
                     /locus_tag="VFC01_09585"
     CDS             complement(11788..13134)
                     /locus_tag="VFC01_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN79920.1"
                     /translation="MTTLTVPRVSGGEEEHGHLEEFRTDPIGLMKRVREECGDVGIFQ
                     LAGKQVVFLSGAEANEFFFRSSDDDLDQAAAYPFMTPIFGEGVVFDTDPERRKEMLHN
                     AALRGEQMKGHATTIEGEVRRMIENWGESGEIDLLDFFAELTIYTSSACLIGKKFREQ
                     LDGRFARLYHQLEQGTDPLCYVDPYLPIESFRLRDEARLGLVALVQEIMTERIANPPK
                     DKGDRDMLDVLVTIKDEDGNPRFSANEITGMFISMMFAGHHTSSGTASWTLIEMLRHP
                     DVYADVVAELEELYADGQEVSFHALRQIPKLENVLKETLRLHPPLIILMRVARGEFEV
                     AGYPIHEGEMVAASPAISNRIAEDFPEPDSFNPGRYEKPRQEDLINRWTWIPFGAGRH
                     RCVGAAFATMQIKAIFSVLLREYDFEMAQPSESYQNDHSKMVVQLAQPAKVRYRKR"
     gene            complement(13131..13955)
                     /locus_tag="VFC01_09590"
     CDS             complement(13131..13955)
                     /locus_tag="VFC01_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN79921.1"
                     /translation="MPRFDPLPDRRPAIVAGASSGIGAATAVELAGRGFPVALGARRV
                     DKCQELVDEIRSNGGEAIALPLDVTDAESVKGFVHRATEELGDIELLVAGAGDTYFGR
                     LHEISTDTFESQVQIHLIGANRLATAVLPGMVERQRGDLIFVGSDVALRQRPHMGAYG
                     AAKAALVAMVTNLQMELEGTGVRASIVHPGPTKTAMGWSLPAEAIGPALEDWAKWGQA
                     RHDYFLRPQDLARAIAFVAETPRGGFIANMELQPEAPLTTAKERQQLKLGPEGMPS"
     gene            complement(13956..15158)
                     /locus_tag="VFC01_09595"
     CDS             complement(13956..15158)
                     /locus_tag="VFC01_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN79922.1"
                     /translation="MTVPTSDLVLDPYDYEFHEDPYPYYKRLRDEAPLYRNDELKFWA
                     LSRHADVLQGFRNSTALSNKYGVSLDPISRTLDAHKVMSFLALDDPAHLRLRTLVSKG
                     FTPRRIRELEPRVTEIAVQHLDSALQSDTFDYVDEFAGKLPMDVISELMGVPEPDRAR
                     IRALADGVMHRDEGLADVPASAMQASADLMVYYAEMVAERRKKPSDDLTSALLEAEAD
                     GDRLTDDEIMAFLFLMVVAGNETTTKLLANAAYWGWKNPDQLAPVYADHSRIPLWVEE
                     TLRYDNSSQILARTVAGELTLYDTTLGDGDVLLLLPGSANRDDRVFEDADEYRIGRDI
                     GSKLVSFGSGAHFCLGAHLARMEARVALTELFKRIRGYEVDEANAVRVHSSNVRGFAH
                     LPMTVEVR"
     gene            complement(15155..>15646)
                     /locus_tag="VFC01_09600"
     CDS             complement(15155..>15646)
                     /locus_tag="VFC01_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN79923.1"
                     /translation="MLRESSYADLTVRAVAARAKVAPATAYTYFSSKNHLIAEVYLDL
                     VRQVPYFTDVNDSRAHRVGQALRALTLMIADEPEVAAACTTALLSNNDQAVRAVRDRI
                     GAEIHRRIKSAVGPDADPRTVSALEMTYFGALVHAGSGVVTYHQIADRLSYVVGLILG
                     DDE"
BASE COUNT         2524 a         5180 c         5100 g         2842 t
ORIGIN      
        1 tgtgctcgct ccaccgcaga tcgcgcagca gctcgactga gcgtcggcga gatactgccg
       61 aatgtagtga tcttggtagg tcgcgattcg cgcctcgccg gattcgagcc aacgtttaat
      121 actgttgtca cgccggaccg aaccctgaca tgcattgctg cagtagacct tctggctgcg
      181 tttcgacatt gctgccccgc aaccgcgaca ctgtctcatg ctccgaagct acgaccggct
      241 accgacaaca agtcgccgga tttgagccgc gtctagtgga gccccctgtc aggattgaac
      301 tgacggccta cgctttacaa gagcgttgct ctaccactga gctaaggggg cctgcaggcc
      361 gagtttatcg ggtggctcgg gtgggcatcc cggtgcccgt agacgaaagg ctgcgggctg
      421 ttgagtgtca aagacgattt ggtgagcgat ttcccgaatg tgtatccggg gttggcgcgg
      481 cctgacatcg agcggctact gactctcctt gacgagagcg cgagcaccga gggccggttg
      541 agcctcagca tcgcgaccgc cctcaacccg ctggtgcccg acgtcgcacg tcgcatcgag
      601 tcctacaagc aatcgggtga cgtcgatgag tacgtgcgca tgttgcgcgg cgcgaccgtc
      661 ctgctcctcc agaagtgggt accgaacggc cgaccgccgg ctcccgaaac catctcaaac
      721 agcatcgagg gctgacgtca ttcgtcggcg tcgatgaccc gctgcgacgt caccggcctt
      781 gttgtcggcc gcccgcgtgg ccgacggcct gggaacggga tgcggtcggc gatattgctc
      841 aacggattga ctacctggct gagcgtgatc accgcttccc gcagcgcttc gatcgtcggc
      901 tccagcgctt ccaacccggg cgccagtcga ttcaaggtgt ccgccaccgt cgcgagctgc
      961 tcgagcggac cattgcgagc ggtgagcttg tcgatcactc cgccctcagc cagcagccga
     1021 tcagccacac cgtcctcggc cagcagccgg tcgagcaacc cgtcctcggc gagtacctgg
     1081 tcgaccagac cacccggcgc gatggcgcgc atcagcccac tgttctcctc cgtgagccgg
     1141 tccagcacac ctcccggcgc ggtcaaccgg tccaccaagc catcaggctg caaaaggcga
     1201 tccaccggtc cgccaggcgc gagcgcccgg cccagcggct ggtcctcgtc catcagcctg
     1261 gccagccggt tcgcccgttc gacggcgtcg tcgatgccca acatgtgagc taccgatccc
     1321 gaccctgatc gggcccgacc gacggcgcta ttggcgatgt cgaggccggc gtcggcgacc
     1381 gccagcccga cccgcaccgg agcggtcgcg agatccgtca gcgttttgcc gaggttcatg
     1441 tcgctcagtc tatgggcgta cgctcggcgc acccgaagct tgggcgtctt gcctgaaaac
     1501 aaactcacag gaagttgaca ggcatcatgc aggataattc caagcgagat cgtggacatt
     1561 agcgggatgg cgatggctgc actacccgag aatctgcccg aggcgcgcat cctcgtagtc
     1621 gacgacgagg ccaatatcgt cgagctcctc tcggtgagcc tgaaattcca gggcttcgaa
     1681 gtccataccg cgtccaacgg gcccgccgcc ttggacaagg cccgtgaaat acgccccgac
     1741 gcggtcatcc tcgacgtgat gatgcccggc atggacggct ttgggctgtt gcgccggctg
     1801 cgcgccgacg gcatcgacgc gcccgccctc ttcctcaccg cccgcgacac cctgcaggac
     1861 aagatcgccg gcctcacgct cggcggcgac gactacgtca ccaagccgtt ttccctggaa
     1921 gaagtggtcg cgcggctgcg ggtgatcctg cgccgggcag gccgtggtgt cgaagagcag
     1981 cgcccctccc ggctcacctt cgccgacatc gaactcgacg aggacaccca cgaagtgtgg
     2041 aaggccggcg agccggtttc gctgtcaccg accgagttca cgctgctgcg ctacttcatc
     2101 atcaacgcgg gcactgtcct ctcgaagccc aagattctcg accatgtgtg gcgctacgac
     2161 ttcggcggcg acgtgaacgt cgtcgagtcc tacgtctcgt atctgcgccg caaagtcgac
     2221 accggcgaga agcgtctgct gcacacgttg cggggcgttg ggtacgtcct gcgtgaaccc
     2281 cgctgatctg ttcgcgacaa taggggtgtg gcggcactgc gcgggcgagg cataccccta
     2341 agggtcgggc tggtcgctgc caccctggtg ctggtggcct gcggcctgct ggcgtcgggg
     2401 atcgcagtca ccacgatcct gcgccacgac ctcaccaacc gcgtcgacga gacgcttctc
     2461 gacgcgtccc gcagctgggc gcaggccccg cgtcgcatgc cggcgccgcc gatcgagggg
     2521 cccaaccccg cccggccgcc gtcgaacttc tatgtgcgcg gtatcgaccc cgacggccgc
     2581 atctggatgg cggtcaacga ccgcgccgcc gagcccgcgc tgcccgccga caacgacgtg
     2641 ggaccggtgc cggtcaccgt cgggtccatc gacaactcga acgtgcaatg gcgggcgatg
     2701 agtgtgcgcg ggttgcgcgg tgagctcacc accgtcgcga tcgacctgtc ggatgttcag
     2761 acgacggtgc gcgcgctgat ctatgcgcag gtcggcatcg gcgcggcagt gctgctggtg
     2821 ctcggcgtcg ccgggtattg ggtggtgcat cgcagcctgc gtccgctgac cgaggtggag
     2881 cagacggcag cggcgatcgc cgccgggcaa ctcgaccgtc gtgttcccga acgcgacgcg
     2941 cgcaccgagg tcggccgatt gtcgttggcg ctcaacggaa tgctggcgca gatccagcgg
     3001 gcggtggcgt cgtcggattc gtcggccgcg gccgcgcgca cctccgagga caggatgcgc
     3061 cgattcatca ccgacgccag ccacgagctc cgtacgccgc tgaccacgat ccgcggcttc
     3121 gccgagctgt accggcaggg cgccgccaat gacgtcgaga tgctgatgag ccgcatcgag
     3181 agcgagtctc gtcgcatggg tctgctcgtc gaggacctgc tgctgctggc ccggctcgac
     3241 cagcagcggc cgctggaacg ccatcgcgtc gacctgctcg ccatcgccac cgacgccgtg
     3301 cacgacgcgc aatccatcgc gccctccgaa cgcaagatcg ccatggaggt gttcgacggg
     3361 ccgggcaccc cggaggtgct tggcgacgag gcccgcctgc gccaggtgct gggcaacctg
     3421 gtcgcgaacg cgttgcaaca cacaccagac agtgcgggca tcaccgtgcg cgtcggcacc
     3481 gacaacgacc acgcagtgct cgaggtgtgc gacgagggtc ccggcatgac caaggaggac
     3541 gcgcaccggg tgttcgagag gttctaccgt gccgactcgt cacgaaccag ggccagcggc
     3601 ggcaccggct tggggctgtc gatcgtcgac tcgctggtac acgcgcacga cggcacggtc
     3661 agcatcacca ccgcgcctgg gcagggttgc cgcttcaagg tcaacctgcc gcgcatcgcg
     3721 gacgtgccgg ctcacgtcag ctgagcgaga gcctctttga ttttcgcctg agcctcgtcc
     3781 agggattcct gtgacgggtt gtggtcagcg ttggcgaatc cgaagtcact gaggtcgtgc
     3841 gcggggaaga cgtgcacgtg cagatgcggc acttccagtc ctgcgatgat cacgccggcg
     3901 cgctcgacgt cgaatgcctt ggtgaccgcc ttgccgatca gctgcgagac ggccatcacc
     3961 ttgttgaacg cgggggcatc gacgtcctgc cactgatcga tttcggcgcg cggcacaacg
     4021 agcgtgtgac cgtgggtgat cggcgcgatc gtcaggaagg cgacgacgtc atcgtcctcg
     4081 tagacgaacc tgcccggaat ctcgccgttg atgatcttgg tgaacacggt cgccatgggg
     4141 ttgagcattc cagcaagctc ccagccttgg cgcagtgggg tagcagctta tttagagacg
     4201 ctcgacacca tgaccgacct ggcggcgccg cgcacggccg aggtatccga gcggccaacg
     4261 cgcagctgcg tcctcgacat cgtgatcccg gtctacaacg aggaggacga ccttccggga
     4321 agtgtccgcc ggctgcaccg ctatctgacc gccgaggtgc cgtatccgtc gcgaatcacg
     4381 atcgccgaca acgcaagcat cgatcgcacg ttggcggtgg cgaaggagct ggcgtcggag
     4441 ctgccggacg tcgacgtcat ccacctcgac aggaagggcc gcggcggcgc gctctacacc
     4501 gcgtggatgg cttcggacgc cgaagtggtc gcctacatgg acgtggacct gtccaccgat
     4561 ctgtcggcgt tgatgccgct tgtcgcgccg ttgatttcag gccactccga tgtcgccatc
     4621 ggttcgcggt tggcggcctc atcgcgggtg gtgcgcgggc cgaagcgcga attcgtctct
     4681 cgcagttaca atttgatctt gcgaggggtg ctcggcgcga agttctccga tgcgcaatgc
     4741 ggcttcaaag ccgtgcgtgc tgacgtggcc cgccaactgc tgccgcacgt cgcggacacc
     4801 ggctggttct tcgacaccga gctgctggtg atcgccgagc gtgccggtct gcgcatccac
     4861 gaggtgcccg tcgactgggt cgacgacccc gactcgcgcg tcgacatcgt ccgcacggca
     4921 atcaacgacc tcaagggctg ctggcgtgtg ggccgtgcac tggcgagtgg tgcgctgcct
     4981 gtccgcgagc tgcagcgcag cctcggccga gagccgttgg tgccgggcgt gccgcgcggc
     5041 atggtcggtc agatggtccg gttcggcatc gtcgggatcg cgagcaccgt cgcgtttgcg
     5101 ttgttgtatc tgctgctgca cccggcgatg ggtgcccagg cggcgaacct gaccgcgctg
     5161 ttgcttaccg ccgttgccaa tacggctgcc aaccgggcct tcacgtttgg catccggggg
     5221 cgaaacggcg tggcgcgcca ccagttgaac ggactgctgg tgttcgcgtt ctgtctggcg
     5281 atcaccagtg ggtcgttgtt cgtgctgcac cggtttgacc cggcggtcgg caaggtcgtg
     5341 gagttgtcgg tactcgtggc ggcgaatctc gtcgcgacgc tgatccggtt cgtcgcgctg
     5401 cgaagggttt tcagcgcgta gcgcaatgtc gcgccgcctg cgcgctgtgt cgccctagct
     5461 aatcatcgcg tggatctcgt cgacggacca cggcggcgag tcctggtgat cgcgataccc
     5521 gaggatcgac ggcgtctgac ggtcgaagcg gcccacgaac ccgcccgccg cgatgaagat
     5581 ctggcccgtg acgtccttcg cgagatcgct ggcgaggtac gcatacgtcg gtgccacaga
     5641 ctcgggcggg ggcgcatcca gcgctgcctg catgctggct tcgtccaaca tgccgcgtcg
     5701 gtgcaattcg accatgtgag cctcgaactc cgaacccgtc gacagccgcg tcttggcgcc
     5761 agggcagacg acgttcgcgc gcacgccgtg ctccttcaat tcggccgcga gggccatcgt
     5821 caacccgttg acgccgccct tgccagccgg atacccggtc ccgccgtaat cgccgaggaa
     5881 cgcgaacgaa ctggtgttga tgatcgaacc gccgccctgg cccaccatct tcggcgccgc
     5941 cgcgcggcac gtctcgaacg tcgtgcccag gtgcgcgtcg accagttcac ggaattgcgc
     6001 gctggtgact ttcaggatcg actcgctggc aagccccgcg gtgcccgcac agttgaccag
     6061 gacgtcgatc tggccgaact ccttgatgca ggtgtcgatc aacgcgtcgg caaccgctgg
     6121 gtcggcgggc gaacccgaaa atgcgacggc acccgtaata tgttgcgccg cttcggttgc
     6181 cgcgtcggcg tcgcgaccgt tgatcaccac acccgcgccc tgcgcggcca gcagttcggc
     6241 cacagctaga ccgacgccgc gagagccgcc gaccacaacc gcgccgcgac ccgccaggct
     6301 aattgccctc gccctcctgc gcgaactgca tggcatccat gttccagtac ccacgcaggt
     6361 tcgtgatcag gccggcgtcg ttgaccttgt acgtgaacac gccgcgcacg gtcgccgtga
     6421 acccgttcgg aaacctggtg cgcagcacca gaatgtaggc gatttcggtc ggcgagctgg
     6481 acgggaacgt ctcttcccgc gtgaccgtga gttggttggg gccgatgttg gtgtcgaaga
     6541 acgcggcgac ggcatctttg ccgcgcacgc cgccgccgcc cggattggtg accgcgtcgc
     6601 cgatcgggtc ttcgatgacg atgtcgtccg ccatgagcgc gagccagcct tctttgtcgc
     6661 ctgcctgcac gcagcgccac gatgactctg aggcaatcac gaccggtgtc ttctcgaccg
     6721 tctcctggga catttcactc ctttgttaga tggcgcaggc ggcgcgggcg ccgtcctcgg
     6781 tggccttccg gatcagccca agcccggtgc agtcacccgc ggcgtgtacg gccgcattgg
     6841 gcagggcggc gacgagcttc tcgtaaagtt cggtgttcgg ctgcagcgtt cccgcgacga
     6901 cgacgctgtc ggcggcgacc tggcggctgg taccgccgta aggcgtgaag acgacactgt
     6961 cgggcaggat ctgctcaacc ggtgcccgca catgtaccgc gacgccgagc cggtcgagcc
     7021 ggtccatatg ttcggtcttg cgcttgtttc cgagttccgg cgcgatcgtc ttgtcggctt
     7081 ggagcaccgt caccagacgg ccacgcgcgg caaggaattc ggcgagctcc agcgcgacaa
     7141 gatcggcgcc gacgatcaca acgcgatggc cgagcggcat ccacgcttta gaggccaggc
     7201 ggacagccgt agggcgaacc agtcgttgtc gccagccgcc gaaccgttcc cgcagcgcct
     7261 ggccggtctg cacatgcggc aggttcgaac cgggaatagt cggtagcacc acctggccgc
     7321 cgttggctac caccgccgcg tccggccgca ggtcgaccac gtcgtcggcc gaaacctccg
     7381 ttcccagttc aaccttcacc gagctgcggc cgacctcgtc gcgcagccac cgcaggaacg
     7441 gctcgttctc ggggtgcagg atcgacgccc acagcagcgc gccgcctgtc tggctggtgc
     7501 gttcgaacag cgtgacctga tgtccccgtt cggcggccac tcgggcggcc tccatgcctg
     7561 cgggcccgct gccgatcacg accaccttct tcgggcgcgg cgccctgggc gcggcaagtt
     7621 cgcgctcctt gccggtgcgg gggttgaccg cgcagtcgac cgagaaccgc tgttccatgg
     7681 cgtcgataca gttctcgcag gagatgcatt tgcgcacgcg gtcggcatgg ccggtggcaa
     7741 ccttggtcaa cagctcggcg tcggcgagca tcgggcggcc cattgcgatg aagtccgcgc
     7801 gaccgtcggc gaggatctgc tcggcgcgct ccggatcgtg gatgcggccg acggcgatca
     7861 ccggcacgtc gaccacctgc ttgaccgcgg ctgcggcgcc gacgttgagt gcgtcgccgt
     7921 cgtcagctga gttgaccatg ccggtgacca gccggtcgat cacaccgccg ctgacgtgaa
     7981 acgcgtccac cccggcggct accagttgcg gtgccacctg ggcggtttcg aagatcggcc
     8041 ggccacccgc cacccgctcg tagccggaga tgcgcaatgt gatcggcagc gcgtcaccga
     8101 tctcgcttcg gattgcagcg agcgcctcca gcacgacccg gatgcggccc tttgccgagt
     8161 cgccccggta atcgtcggtg cggcggttgc gctgcggcgc gaggaacgag cccaggaaca
     8221 tgtagccgtg cgcggcgtgc agttcgatgc cgtcgtagcc ggcttcggca gctcgccgca
     8281 ccgccccctt gaacaagtcg aacacctcaa tgagttgttc gggggtgacc tcggctgagg
     8341 gcctgccggt caaatacgac ggaatgaccg acggcccaat cgaagtcacg ccgtacattt
     8401 cgggtccgag tccgtcgggc ccggcgtgca cgatctgcgg ctggatcttg gcgccgtgct
     8461 cgtgcaccac ctcaaccaac gcgcggtggg cgtcgacggc cgcgtcagtg cccaggtgca
     8521 atccgcccgg cgtctcgggc tggcggtggt cgatcccggt ggcgcccacg gtgatcagcc
     8581 cgacaccgcc cttggcacgg gcggcaaagt agtcgcgggt gcgctccgac ggtagcccgt
     8641 ccgatgtgcc gtacatggtc tccatcggag acatcaccag ccggttgcgc agcgtcatcg
     8701 agccgatctg gcccggctgc aacaagtgtg agaagtgctt caccgtgcga atcggccggc
     8761 gaacgcgcgc agcggcaggt cggcgcttgt gacgatgccc ggcgccgcat cgacaaccgc
     8821 gcgtatggcg ttcagtgcgg gcatacccgt cacggtcatt ccgatcttcg cgaacgactc
     8881 gaccgatgag aaatccgtgc cgggcttggg cagaatcatg tgcttgctgt agatgcaggg
     8941 atcgccgtcg atcttggtga tgtagcaccc ctggatgtcc caatgtggat cggtgtgcgg
     9001 ggtcatctgc caatccatcc gcagttcgac ccgcttgacg tcgtccacca ttcccacgta
     9061 tcccagcacg cagccaccga gtgatcccgc gggcaattga taccagccga ggtcgacatc
     9121 ctttgtgcag gcgcctaatt ggtaggagaa cttcactccg tttggtccgg ggtcaagttc
     9181 gatgtcgagg cagtcggcca tcagccggac gccgtcttcg aacaccctgg tacctaattc
     9241 gaggagctgc ggcacctcgg ggtcgtcgac gggtcggccg aagccgcagt tctgccatgt
     9301 ctccgcagag tgatgacacg acacgtcgac cgactcgatg caggttacgc tctcgatgtc
     9361 agcgacgtcg gcggaatgca cgatcgtcag gatctgcgcg aggcccgggt tcatccctgt
     9421 tccgtagagc gtggagtttc cgcgttcgca ggcagcttgc agaatctcgg actcggtaga
     9481 tccgttgcgc cggtggttgg tgttgcggtg gtggccggtg atccagtccg cggtggtgac
     9541 gatgttgatg ccggcttcca acacctgcgt gtagagctcg atgtcgggcc aaacaccgtg
     9601 gaacgtgacg caatccgggc gggcggccag aatctccttg acggtgccgg tcgcggtgac
     9661 accgaccggg ccgatgccgg cgatctcgcc gacatcgcgc ccaaccttgt ccggcgaata
     9721 gcagtgcagc cctgccagtt ccaggtccgg atgattctgg atgcgcctga tcatctcggt
     9781 gccgacgttt cccgacgcca cctggaacac ccttacgggg ctgctcatat cgttctacct
     9841 atcgtcgtcg gtgtagcgaa tgacgccgcg aatgttcttg ccgtccaaca tgtcctggta
     9901 gccctcgttg atctgctcca gcttgtactg ccgagtgatc atgtcgtcga tgttgagctt
     9961 gcccgccttg tacatcgaca gcagttgcgg gatgtcgtaa tgcgggttgc cgccgccgaa
    10021 gatggtgccc tgcaggttct tctgcatcag cgtgagcatc gcgaggttca gcgtgacctg
    10081 ggtgtcgagc aggctgccga tcgcagtcgc gacgcaggtg ccgcccttgg cggtgatcat
    10141 caggtagttg tcgatgtcct cgcccttgag ctcaccaacc gtgatgatga ccttgtgagc
    10201 catgaagccc gcggtcacct cggcgatgcc catcagagcg gtgttgatgt ccggatacac
    10261 atgcgtagca ccgaatttca gcgcagcgtc gcgtttccac tcgaccggct cgatgacgaa
    10321 gatgtgccgc gcgccggcgg tgacggcacc ctgcagcgcc gacatgccga cgccgccgac
    10381 gccgacgatc gcgacgtcct ggccgggccg gatgtcggcg gtgcgcaccg ccgagccgta
    10441 gccggtggtc acgccgcagc cgacgaggca ggccacctcg aacgggatcg acgggtcgat
    10501 cttcaccacc gagctgcggt gcaccaccat gtacggcgag aaggtgccca acagtgtcat
    10561 cgggaatacg ttttgccccc tggcctgaat ccggaaggtg ccgtcggaaa ccgcagcgcc
    10621 gttgagcagg cccgcgccca ggtcgcacag attgcgcagc ccggcctgac acgacggaca
    10681 cttgccgcac gaggggatga aggacagcac gacgtggtcg ccgggcgcga tgtcctcgac
    10741 tccgggcccg acttcggtga cgattccggc gccctcgtgt ccgcccagca cggggaagcc
    10801 gcccatcggg atgccgccgg tgaccaggtg gtggtcggag tggcacatcc ccgacgcctc
    10861 catctggatc ttcacctcgt ccttgacggg gtcgccgatc tcgatctcct cgatcgacca
    10921 cggctgattg aactcccaga tgagagcgcc ttttgtcttc atcgcaaact cgcttcctct
    10981 cgaatgcccc cgcgacagac taagttatct acgtcacata gctaccagat cggtgagggg
    11041 gactttccga gcgggtagta gccgggaact ttctcgcccg ccatgccgcg ttcgatgcgt
    11101 ttctgcatgc cctcggacag cttgccggcc ttgatcatgt ccatgtacag cgccgagacg
    11161 tgaccgaagt cgaagaagtc acgctgccac tcgatcttgc cctcggcgtt gagacggaac
    11221 cagctcccgc cgatgccgta cacctcctgc tcggtgccat caccatccgt ggcgacctgc
    11281 ttccagaacc cgacgacctc accgatcttg tcgtcgatga gcaccctctg gtatgggtag
    11341 cgccagccct cgaggccctc catctcctgg ccgagcgcga tgtcgcggat ctcgtcgatg
    11401 ccgacgcaca tcacgtcctc cttcgggccg atgttccagc cgtaggtggc gtcgtcggtg
    11461 tagaaatcgg ccaggccccg ccagtcgccc ttgcgttcgg cttcgcggtt gacctccagc
    11521 caccgctcga cgaaatcctc caattgttcg cggggtagtg acggcattgt cagtcttcct
    11581 tttctctgat ggacagggcc tgagtaggac aggcccacac ggcttgctcg atctgtgccc
    11641 gatcctcttc gggcggttcg tcattgagga tttcgacctg gccgcgtttg ggcaccttga
    11701 agtaatcggg cgcttcgagc tcgcacatcg cgtggccctg gcacaggtcc aggtcgactt
    11761 ctactcgata gcccacggtt ggcctcctca gcgcttccgg tagcgcacct tggcgggctg
    11821 ggccagctgc accaccatct tcgagtggtc gttctggtaa gactccgacg gctgcgccat
    11881 ctcgaagtca tactcgcgca acagaaccga gaagatcgcc ttgatctgca tggtggcgaa
    11941 cgccgcgccg acgcagcgat ggcgcccggc gccgaacgga atccacgtcc accgattgat
    12001 caggtcttcc tggcggggct tctcgtatcg gccggggttg aacgaatcgg gttcggggaa
    12061 gtcctcggcg atgcggttgg agatcgcggg cgacgccgcc accatctcgc cctcgtggat
    12121 cgggtagccc gctacctcga actcccctct ggccacccgc atcaggatga tcagcggcgg
    12181 gtgcaaccgc agcgtctcct tcagcacgtt ctcgagcttc ggaatctggc gcagcgcatg
    12241 gaaactgact tcctggccgt cggcgtagag ctcttcgagt tcggcgacca cgtcggcata
    12301 cacgtcggga tggcgcagca tctcgatcaa cgtccacgac gccgtgccgg agctggtgtg
    12361 gtgcccggcg aacatcatcg agatgaacat gccggtgatc tcgttggcgg agaaccgcgg
    12421 gttaccgtcc tcgtccttga tggtgaccag gacgtcgagc atgtcgcggt cgcccttgtc
    12481 cttcggcggg ttggcgatcc gctcggtcat gatctcctgg accagcgcca ccagcccgag
    12541 ccgggcctcg tcgcgtagcc ggaaactctc gatcggcaag tacgggtcga cgtagcacag
    12601 cggatcggtg ccctgctcca gctggtggta gagccgcgcg aagcggccgt cgagctgttc
    12661 gcggaatttc ttaccgatca ggcacgccga cgacgtgtag atggtcagct cggcgaagaa
    12721 gtccagcagg tcgatttccc cgctctcgcc ccaattctcg atcatccggc ggacctcgcc
    12781 ctcgatcgtg gtggcgtggc ccttcatctg ctcaccgcgc agggcggcgt tgtgcagcat
    12841 ctcttttcgg cgctcgggat cggtgtcgaa caccacgcct tcgccgaaga tcggcgtcat
    12901 gaacggatac gccgcggcct gatcgagatc gtcgtcgctg gaccggaaga agaattcgtt
    12961 ggcctccgca ccggacagga acaccacctg cttaccggcc aactggaaaa tgccgacgtc
    13021 gccacactcc tcacggacgc gtttcatcag cccgatcgga tcggtgcgga actcttcgag
    13081 gtggccgtgt tcttcctccc cgccggatac ccgcggcacg gtcagggtgg tcatgacggc
    13141 atcccctctg gtcctagttt gagttgctga cgttccttgg ctgtggtcag cggcgcttcc
    13201 ggctgaagtt ccatgttggc gatgaagccg ccacgcggcg tctcggcgac gaacgcgatg
    13261 gccctcgcga gatcctgcgg gcgcaggaaa tagtcgtggc gggcctggcc ccacttggcc
    13321 cagtcctcca gggccggtcc gatcgcctcc gcgggcaggc tccagcccat cgccgttttc
    13381 gtcggcccgg gatggacgat cgacgcgcgc acgccggtcc cctcgagctc catctgcagg
    13441 ttggtcacca tcgcgacaag cgcagccttg gccgcgccgt aggcgcccat atgcggacgc
    13501 tggcggagcg ccacgtcgga cccgacgaaa atcaggtcac cgcgctggcg ttccaccatt
    13561 cccggcagca ccgcggtggc caaccggttg gcgccgatga gatgtatctg cacctgcgat
    13621 tcgaaagtgt ccgtgctgat ttcgtgcagc cggccgaagt acgtgtcgcc tgcaccggcg
    13681 acgagcagct cgatgtcgcc gagctcttcg gtggcgcggt gcacaaaccc cttcaccgac
    13741 tcggcgtcgg tgacgtcgag ggggagcgcg atagcctcgc cgccgttgga ccggatctcg
    13801 tcgaccagct cctggcactt gtcgacacgt cgggcgccca gcgcgacggg aaagccgcgg
    13861 ccggcgagct cgacggccgt cgcggcgccg atcccggacg acgcgcccgc gacgatcgcg
    13921 ggcctgcggt cgggaagtgg gtcgaaccgg ggcatttagc ggacctccac ggtcatgggc
    13981 aggtgagcga atccgcggac attgctcgaa tggactcgga cggcgttggc ctcgtcgacc
    14041 tcgtatccgc ggattcgctt gaacaactcg gtgagcgcca cccgggcctc catccgggcc
    14101 aggtgcgcac cgaggcagaa gtgggcgccg ctgccgaaac tcacgagttt cgaaccgatg
    14161 tcgcgaccga tgcggtattc gtccgcgtcc tcgaacaccc ggtcgtcgcg gttggcggag
    14221 ccgggcagca gcaacagcac gtcgccgtct ccaagcgtgg tgtcgtagag cgtcagctcg
    14281 cccgccacgg tacgggccag tatctggctg gagttgtcgt agcgcagcgt ctcctcgacc
    14341 cacagcggga tcctggagtg gtctgcgtat accggggcca actggtcggg gttcttccaa
    14401 ccccaatacg ccgcgttggc aagcagtttc gtggtcgtct cgttgcctgc gaccaccatg
    14461 aggaacagga acgccatgat ctcgtcgtcg gtcaggcggt cgccgtcggc ctcggcttcg
    14521 agcagcgccg acgtcaggtc gtcgctcggc ttcttgcggc gctcggcgac catttcggcg
    14581 tagtagacca tcaggtcggc cgaggcctgc atggccgacg ccggcacgtc ggccaggccc
    14641 tcatcgcggt gcatgacacc gtcggccaag gcgcggatgc gcgcccggtc cggctcgggc
    14701 acccccatga gttcggagat gacatccatc ggcagcttgc ctgcgaactc gtcgacgtag
    14761 tcgaaagtgt cggactgcag cgcggagtcg aggtgctgca cggcgatttc ggtgacgcgc
    14821 ggctccagct ctcgaatgcg gcgcggggta aagcctttcg agaccagggt gcgcagccgc
    14881 agatgggccg gatcgtcgag ggcgaggaac gacatgacct tgtgcgcgtc gagcgtgcgc
    14941 gagatcggat cgagcgatac gccgtatttg ttcgacaacg cggtgctgtt gcggaatccc
    15001 tgcaacacgt cggcatgacg cgacagcgcc cagaatttca gctcgtcgtt gcggtagagc
    15061 ggggcctcgt cgcgcagccg cttgtagtac gggtacgggt cttcgtggaa ttcgtagtcg
    15121 tacgggtcga gaaccaggtc actggtcggg actgtcattc gtcgtctccc aggatgaggc
    15181 cgaccacgta ggacagccgg tcggcgatct ggtggtacgt gactacgccg ctgcctgcgt
    15241 ggaccagggc gccgaagtac gtcatctcca gagccgacac cgtgcggggg tcggcgtccg
    15301 gccccacggc cgacttgatg cggcggtgta tctcggcgcc gatgcgatcg cggaccgcgc
    15361 gcacggcttg gtcgttgttg ctcagcagcg ccgtcgtaca ggcggccgcg acttcgggtt
    15421 cgtcggcgat catcagggtc agcgcgcgta gcgcctgacc gacgcggtgc gcccggctgt
    15481 cgttgacgtc ggtgaagtac ggcacctggc ggacaaggtc cagatagacc tcggcgatca
    15541 ggtggttttt cgacgagaag taggtgtagg cggtcgccgg ggcgaccttg gcgcgcgcgg
    15601 ccactgcgcg cacggtcagg tcggcgtacg acgactcgcg cagcat
//
LOCUS       DASRJA010000215         9894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2684320,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000215 DASRJA010000000
VERSION     DASRJA010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9894
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2684320"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFC01_09605"
     CDS             complement(<1..328)
                     /locus_tag="VFC01_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HZN79924.1"
                     /translation="MSANRIAHSLTWLGGGEWRELGERDERSAHVTAGVVVLLAAALA
                     WLVSTLAIAAATQVSMAAIVPLTLVFGLVVGAVSRAVASGATRSWSGVIGRAAVAVAV
                     GAVVGEL"
     gene            complement(510..806)
                     /locus_tag="VFC01_09610"
     CDS             complement(510..806)
                     /locus_tag="VFC01_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79925.1"
                     /translation="MTHWTRSVAVAAASVFALAAVPAGLVTIHAPGIANADVCVSGGR
                     RVSVSGCANIADAVAPYVPPPAYYAPMPYDPPPPPNVTGCVSYNGRWVNAAGCN"
     gene            complement(882..1274)
                     /locus_tag="VFC01_09615"
     CDS             complement(882..1274)
                     /locus_tag="VFC01_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZN79926.1"
                     /translation="MLLSGIFTRRAAAGLIGTGAVTGAMLIGGSALAFAEPAPEPPPS
                     PAPGCTAADLAEASGIVGTAMGVYLFSHPDVNNFFTSLRGLPNEELRGRVQTYMDANP
                     AVEAEINGIRQPVTDLRNRCDAPAPMGE"
     gene            complement(1415..2434)
                     /locus_tag="VFC01_09620"
     CDS             complement(1415..2434)
                     /locus_tag="VFC01_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZN79927.1"
                     /translation="MSTAMRVPETTTISGDELSADDAVTTLRRYGRWNLVKNSFIRFR
                     YADGFTNARALALQLCLSFIPLIIAVVGLASAVHQEKVGQVIIQTLGGVLPGKGPGDM
                     VEQAAQRTHDQGVTGGQLALWLGLLAAIIALTTAMGQIERGANRIYGIERDRPALRKY
                     GRALVMALTAGLLLQLGFLVIVAGDALGEALAAAYGWGETFERAWTIGSWPAGVALAW
                     ASFTVVLERAPRRRQPGYSWLAVGSGVSLALWLLLTGLLALYVVKSGSFGTTYGPLTG
                     IFALLLWANLSSVALFLGVAFAAQLEAVRAGTPAPASDDPLTTERPTTPMEPVVMSAT
                     PSHYS"
     gene            complement(2449..3345)
                     /locus_tag="VFC01_09625"
     CDS             complement(2449..3345)
                     /locus_tag="VFC01_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HZN79928.1"
                     /translation="MTRAAIILNPATADVSALREAVEKTMATAGWSTPMWLETTPEDP
                     GAAMAEAAVAAGVQLVVICGGDGTIMACLGALAGTDLPVALLPLGTGNLLARNLGVPI
                     ELADALAVAVNGEDRRIDLGRVGEQPFSVMAGIGFDAAMMADASEGLKQLAGWPAYVV
                     SGLRHLRDPVMQVQLRIDDNPPLRRPARTVLVGNVGRLQGGLELLPDAAPDDGLLDVV
                     VVAPRTLRDWIRLVWRVFRRDRTQHPHLERFRGRTILIEADRIVPRQMDGEVIDDGRS
                     LYARIQPGALVIRAPQPVATAA"
     gene            complement(3342..3980)
                     /locus_tag="VFC01_09630"
     CDS             complement(3342..3980)
                     /locus_tag="VFC01_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZN79929.1"
                     /translation="MTVGLGYLVTEVLPSTTIGKWDAEVPRRLVEYRQQEGVSESRLI
                     TSLSETPTIVALTAIAAAVFRWKFGRWRESLVVIYAVVGETSIFLATTLLIDRPRPDV
                     PKLDVAPPTSSFPSGHTAAAVCFYGSVAAIILWHTRHRWIKVVAVVVCAAVPLTIAGS
                     RVYRGMHYPTDVVVGMLLGAIWLTVVIFYVRTHDAGGRRDAAVDADTRLVTR"
     gene            complement(4123..4878)
                     /locus_tag="VFC01_09635"
     CDS             complement(4123..4878)
                     /locus_tag="VFC01_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZN79930.1"
                     /translation="MSKRLRQAVHRHLSAADERFGLRLGGGLAGMLVAAVTFALLLVL
                     VRAGWGPLRSLDTAAAEAFNRIDVDQPGVVKAAEVVSDVFDPNVFRVGLTVIAVVYVI
                     HGERHHATWLVVTVFGGAALGFALKEIVGRARPVLPDPVSAAPGLSFPSGHALGASIG
                     CCLLLLITLRFLPRRGRIAAVIAAVLIVGIVALARVVLGVHFVSDVLAGIALGIGWVA
                     VTTWAYVAWRRETGQPVERPAEVGTTEHPAAHR"
     gene            complement(4917..6566)
                     /locus_tag="VFC01_09640"
     CDS             complement(4917..6566)
                     /locus_tag="VFC01_09640"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HZN79931.1"
                     /translation="MALLLGPALRHVPDTTALVWVQTESAATVEVLGCSARTFAVQGH
                     HYALVTVTGLTPDTVTEYDVKVDGELVWPLADSPFPPSVIRTRGPASTHRLQAIFGSC
                     RYPKTDDGKLDSKLGLDALDVYATRMATRPVDDWPEALILLGDQLYADELTPESRQRL
                     AGRRTLMIRRGQRPPDEVVSFAEYERLYRHSWGDPEIRWLISTIPTAMIFDDHDIRDD
                     WNTSASWRADMKEKPWWRDRIRAGLASYWVYQHLGNMSPVELADDEDYQRLVAADGDT
                     WPHLVDLADRADQEVDSSKGIRFSYRWDLGRSRLIMIDSRNGRILDSGERMMIGEREF
                     AWLESQATDGLDELDHLMLASSVPWLMPPAIGDLEAVNERNADRSGLRGRLAEKIRRG
                     VDFEHWPAFYKSFVRLAEMIARVASHPNGPATVSVLSGDVHHSYAARAEWAVESAPPP
                     GATVHQLVCSPVNNYVPLFLEPVFKLSWSRPAAALARWWASLHGVPALPLSWANTRGP
                     LFGNTIATIQVDGRSAEVLFEQPRCSDALDEVGRVALNPSS"
     gene            complement(6620..7579)
                     /locus_tag="VFC01_09645"
     CDS             complement(6620..7579)
                     /locus_tag="VFC01_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZN79932.1"
                     /translation="MAKEFPKQKAGLNYLTEGGQETEILYKYGFELPHFAMFPLLDNP
                     RAMTELSGMYGRYLDTAARHGFGVVIGGLDYRASPDWASLLGYRGEALAQMQMRAIDF
                     LRDVAEPYRGQVPAVLYAGIVGPRGDAYELNRTITAQEAEEYHSEQLATLARASVDLV
                     EAMTFNSVDEVIGLSRAASQVGLPLSVSFTLDNSTSRLLSGPTLKDAIETVDAQTGDD
                     RPAFYGINCSHPLEFMPAIERGSWFERVRVLRPNAAMMDKISLCTLGHLEAGDPAHLG
                     ELMGGLARQYPHLDMWGGCCGTWDTHLDEIARNVHTARDAVPL"
     gene            7795..8724
                     /locus_tag="VFC01_09650"
     CDS             7795..8724
                     /locus_tag="VFC01_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN79933.1"
                     /translation="MRARRIALLVGAATAASWAPLSAVVPSASAQPCPDVEVVFARGT
                     TEDPGLGPTGQAFVDSLRARVGARSMGIYPVDYPATVDFPTALDGIRDASAHIESTAA
                     NCPNTKMVLGGFSQGAAVMGFVTANVIPDGAPDGVPNPMPPDVANHVAAVALFGKPNA
                     RFMRAINQPQIDVGPQYVAKTIDLCVPDDFVCSTGRDFNAHTQYADTGMVDEAATYTA
                     NHVLAALGPAAPPQAPAPAPRQLPAPAAPPQAPAPAAPPQAPTPTAPPQATPPAAPPD
                     ATPPAPPPFNALRPLPPGTLLRCDTVCHIIGPA"
     gene            8886..>9894
                     /locus_tag="VFC01_09655"
     CDS             8886..>9894
                     /locus_tag="VFC01_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN79934.1"
                     /translation="MSAPTIDTAIAAEQPRRSGVARWIRRLAVPIIVGWIALIAFLNV
                     AVPQLEVVGEMRSVSMSPKEAPSVAAMMRIGQVFEEFDSDSSAMIVLEGEAPLGEDAH
                     RFYDDMIGKLRADTKHVQHIQDFWGDPLTRAGAQSEDGKAAYVQVYLAGNMGEALGNE
                     SVSQMQKLVEGLSPPPGVKVFVTGGPALQADQEAAGNKSVRIIEFVTVAVIVLMLLFF
                     YRSIVTVVLVLVMLVLSLSVTRGVVAFLGYYNLIGLSTFATQLLVTLAIAATTDYAIF
                     LIGRYQEARTIGEDRESAFYTMFRGTAHVVLGSGMTIAGATFCLSFTRLPYFQTLGVP
                     LA"
BASE COUNT         1762 a         3348 c         3199 g         1585 t
ORIGIN      
        1 cgagttcgcc gacgacggca ccgaccgcca ctgcgacggc ggcgcgtccg atgacgccgg
       61 accagctacg tgtagcgcca ctggcgacgg cacggctcac cgcgccaacc acgaggccga
      121 agacgagagt cagcggcacg atggccgcca tcgacacctg cgtcgcggca gcgatggcaa
      181 gcgtcgaaac gagccaagcc agtgcggcgg cgagcagcac gactacgccg gcggtgacgt
      241 gcgccgaccg ctcgtcgcgc tcaccaagct cgcgccattc accgcccccg agccacgtca
      301 aagagtgagc gattctgttg gcagacatgg aaactccaaa catctccagg acttgtccgg
      361 cgtgacaggg gcgcgggcct actcatttcg gccaagcaga tttcactccc ccgcatcgcg
      421 ccatcacgcc ggtgcgactg cccgacattc tctcacaggc ccgatcggtg cgcttcggct
      481 gcgagtcaag cgacagtttt gcaggtgccc taattgcacc ccgcggcatt gacccaccgg
      541 ccgttgtagc tgacacaccc ggtgacgttc gggggcggcg gcgggtcata cggcatgggc
      601 gcgtagtagg caggcggcgg aacgtagggc gcaaccgcgt cggcgatgtt cgcgcaccca
      661 ctcacagata cccgtcggcc gccgctcacg cacacatcgg cgtttgcgat gcccggggcg
      721 tgaatcgtga cgagccccgc ggggacggcc gcgagggcga agacgctcgc cgccgcgacg
      781 gccactgacc gtgtccaatg agtcatctcg tttgtctcct caatggcgtg atcggcgtat
      841 ccagccgaag acggttgtgg ggggtagcaa cgcttaggtg actactcacc cataggggcg
      901 ggcgcatcgc agcgattcct caaatcggtt acaggctggc ggatcccgtt gatctcggcc
      961 tcgaccgcag ggttggcatc catataagtc tggaccctgc cccgaagttc ctcgttcggc
     1021 agaccacgga ggctggtgaa gaagttgttt acatcggggt ggctgaatag gtacaccccc
     1081 atcgccgttc cgactatgcc cgaggcctcg gccaggtcag cggccgtgca gccgggggcg
     1141 ggggatggag gaggctcggg cgcgggttcg gcaaacgcca gtgcggagcc cccaatcagc
     1201 atcgcgccgg tcaccgcccc agtaccgatc aatccagcgg ccgcacggcg cgtgaaaatg
     1261 ccagacagca acatgcgttg ctcctttttg ctggtcttgg gccggcaacc ggacggtcac
     1321 caacaatgac agccaaacac aaccgacgac gagaattggc ggttcagcat cctcatccgt
     1381 tttccagccg cagtgcgagc ggtggcgtat cgggctatga gtagtgggaa ggggtcgctg
     1441 acatcaccac gggctccatc ggggtcgttg gccgttcagt cgtgagcggg tcgtcagatg
     1501 ctggggccgg cgtgcccgcg cggacggcct cgagttgcgc ggcgaacgcg acaccgagga
     1561 acagcgccac tgaggacagg ttggcccaca gcagcagggc gaagattccg gtcaacgggc
     1621 cgtaggtggt gccgaaggac ccgctcttga cgacgtaaag cgcgagcagg ccggtcaaca
     1681 gcagccacag cgcaagcgaa acgccggacc ctaccgccag ccacgagtat cccggttgac
     1741 gccggcgcgg cgcgcgctcg aggacgacgg tgaaggacgc ccaggccagt gcgactccgg
     1801 ccggccaact gccgatcgtc cacgccctct cgaacgtctc gccccagccg taggccgccg
     1861 ccaacgcctc gcccagcgcg tcgccggcga cgatgaccag gaatccgagc tgtaacaaca
     1921 ggccggcggt caacgccatc actagagccc gaccgtattt ccgtagtgcg ggccgatcgc
     1981 gttctatgcc gtaaatgcgg ttggcgcccc gctcgatctg ccccatcgcc gtagtcagcg
     2041 cgatgatcgc ggcgagcagg ccgagccaca acgcgagctg gcctccggtc accccctggt
     2101 catgggtgcg ctgcgcggcc tgctccacca tgtcgccggg gcccttgcct ggcagcacac
     2161 cgcccagggt ctggatgatg acctgtccga ccttctcctg atgcaccgcg ctcgccaggc
     2221 cgacaaccgc gatgatcagg gggatgaacg acaagcagag ctgcagcgcg agggcgcgtg
     2281 cattggtgaa accgtcggcg tacctgaacc ggatgaatga gtttttgacc aggttccagc
     2341 ggccgtagcg tcgaagcgtc gtgacagcgt cgtcggcgga gagctcgtcg ccggagatgg
     2401 tggtcgtttc cggcacgcgc atcgcggtac tcatatcggg ctcctcattt aggcggccgt
     2461 cgcgaccggc tgcggggcgc ggatgacgag agcgccgggc tggatccgcg cataaaggct
     2521 ccgcccgtcg tcgatgacct caccatccat ctggcgaggc acgatccggt ccgcctcgat
     2581 caggatcgtg cggccacgga agcgctccag gtgcgggtgc tgcgtgcgat cgcggcgaaa
     2641 gacccgccat accaaccgga tccagtcgcg cagcgtccgc ggtgccacga cgaccacgtc
     2701 gagcaggccg tcgtcgggcg ccgcgtccgg cagcagctcg aggccgccct gcagccgtcc
     2761 gacgttgccg accaggaccg tgcgcgcagg ccggcgcagc ggcgggttgt cgtcgatccg
     2821 cagttgcacc tgcatcaccg ggtcacgcag gtgccgaagg ccgctgacca cataggcagg
     2881 ccagccggca agttgtttca gaccctccga ggcgtcggcc atcatcgccg catcgaatcc
     2941 gatgccagcc atgaccgaga acggctgctc gccgacccgg ccgaggtcga tgcgccggtc
     3001 ttcgccgttg acggcgacag cgagagcgtc tgcgagctcg ataggcacac cgaggttgcg
     3061 ggcgagcagg ttgccggtgc cgagcgggag aagtgcgacc ggcaggtcag tgccggccag
     3121 ggcgccgagg caggccatta tcgtgccatc gccgccgcag atcacgacca gttggacacc
     3181 tgccgccacc gcggcctcgg ccattgcggc gcccggatcc tcgggcgtgg tctccagcca
     3241 catgggcgtt gaccagcctg cagtggccat ggtcttctca acggcctccc gcagggccga
     3301 tacgtccgcc gtcgccggat tgaggatgat tgcggcgcgc gtcatcgggt caccagacgc
     3361 gtgtcggcgt cgacggccgc gtcgcgtcgc ccgccggcgt cgtgggtgcg cacatagaat
     3421 atgacgacgg tcagccagat cgcgccgagc agcatgccga cgaccacatc ggtcgggtag
     3481 tgcatcccgc gatacacgcg gctgccggcg atggtcagcg gcaccgcggc acagacgaca
     3541 accgcgacga ccttgatcca ccgatgccgg gtgtgccaga ggatgatcgc ggcaaccgag
     3601 ccgtagaagc agacggcggc ggcggtgtgg cctgacggga acgacgaagt cggcggtgcc
     3661 acatcaagtt tcggcacgtc tggccgcggg cggtcgatga gaagcgtcgt cgccaagaag
     3721 atgctcgtct ccccgaccac cgcgtagatg acgaccagcg actcgcgcca ccggccgaac
     3781 ttccatcgga acaccgccgc ggcgattgcc gtcagagcca cgatcgtcgg cgtctccgac
     3841 agcgacgtga tgagcctgct ttccgagacg ccctcctgct ggcgatactc cacgagccgt
     3901 cgcggcacct cggcatccca ctttccgatc gtcgtggacg gcagcacctc ggtgacgaga
     3961 tatcccagcc ccactgtcac cgcaacgagg cagacggccg gaatgagaag ccacacgaca
     4021 tcccgtgcga tgctggcaaa acctcgtggg cgcacgtact ggacataccc gcgccgccgt
     4081 taattaactc ccttgacgtg atccgagggg tgaactggca gctcagcgat gcgcagcggg
     4141 gtgctcggtg gtgcccactt cggccggccg ctcaaccggc tgcccggtct cgcgccgcca
     4201 cgcgacgtat gcccacgtcg tcacggccac ccagccaatg ccgagtgcga tgccggccag
     4261 gacatcgctg acgaaatgga caccgagcac cacccgcgcg agcgcgacga tcccgacgat
     4321 cagcacggcc gcaatgacgg cggcgatgcg cccgcgccgc ggcaggaagc gaagagtgat
     4381 cagcagcagc aggcagcacc cgatcgacgc tccaagcgca tggccggaag ggaacgacaa
     4441 cccgggcgcc gccgagacgg gatcgggcaa caccgggcgg gcccggccaa cgatctcctt
     4501 cagcgcgaaa ccgagtgcgg caccgccgaa aacagtgacg acgagccacg tcgcgtgatg
     4561 ccgttcgccg tggatcacat acaccaccgc gatcaccgtg agcccgacgc ggaacacatt
     4621 ggggtcgaag acgtcgctga ccacctcagc ggccttcacc actcccggct gatcgacgtc
     4681 gatccgattg aaagcctccg cggcggcggt gtcgaggctg cgcaatggcc cccagcccgc
     4741 gcgaacgagc actagaagca gcgcgaacgt gactgcggcg accagcatgc cggccagacc
     4801 cccgccgagc cgcagcccga agcgttcgtc ggcggcggac agatggcggt ggacagcctg
     4861 ccgcaggcgc ttcgacatcc atttcgtgtt ccccggccgg ctgttcagta cacccgtcag
     4921 gacgatggat tgagcgccac gcggccgact tcgtcgagcg cgtccgagca acgcggctgc
     4981 tcgaaaagca cttcggcgct gcggccgtcc acctgaatcg tggcgatggt gtttccaaac
     5041 agcggacccc gggtgttcgc ccaggaaagc ggaagcgcgg gtacgccgtg caagcttgcc
     5101 caccatcgag cgagcgcagc cgcgggtcgc gaccaactca gtttgaacac cggctcgagg
     5161 aacagcggca catagttgtt caccggcgag cacaccagtt gatgcaccgt cgcgcccggc
     5221 ggcggggcag attcaaccgc ccactcggca cgcgcggcat agctgtggtg cacgtccccg
     5281 gagagcacgc tgacggtcgc cgggccattg ggatggcttg cgacgcgggc gatcatctcg
     5341 gccaaccgca cgaatgactt gtagaacgcc ggccaatgct cgaaatcgac tcccctgcgg
     5401 atcttttcgg caagtctccc gcgcaggccg ctccggtcgg cgtttcgctc attgaccgcc
     5461 tccaggtctc cgatggcggg cggcatcagc cacggcaccg atgaggccag catcagatgg
     5521 tccaactcgt cgaggccgtc ggtggcctgg ctttccagcc atgcgaactc gcgttcgccg
     5581 atcatcatgc gttcgccgga atcgaggatt cgaccgttcc gcgagtcgat catgatgagc
     5641 cgactgcgac cgaggtccca tcgatagctg aatctgatgc ccttgctgga atccacctct
     5701 tgatccgcac ggtcggccag atcgacgaga tgaggccagg tgtcgccgtc cgccgccacg
     5761 agacgctgat agtcctcgtc gtcggccaac tcaaccgggc tcatgttgcc caggtgctga
     5821 tacacccagt aggacgccag cccggcgcgg atccggtcgc gccaccacgg cttctccttc
     5881 atgtcggcgc gccaggacgc cgatgtgttc cagtcgtccc tgatgtcgtg atcatcgaag
     5941 atcatcgcgg tgggaatggt ggatatcagc catctgatct cgggatcgcc ccacgaatgg
     6001 cggtacagcc gttcgtattc ggcgaagctc accacctcgt ccggcggccg ctggccgcgc
     6061 cgaatcatca gggtgcggcg gccggccagg cgttgtctcg actccggagt gagttcgtcg
     6121 gcgtacagct ggtcgccaag caggatcagc gcctctggcc agtcgtcgac cggccgcgtg
     6181 gccattcggg tggcatacac gtctagcgca tcgagcccaa gtttggagtc gagcttccca
     6241 tcgtcggtct tggggtatcg gcatgaaccg aagatcgcct gtagccggtg agtcgacgcg
     6301 ggcccgcggg tacggatgac gctgggcggg aacggcgagt ctgcgagcgg ccaaaccaat
     6361 tccccgtcga ccttgacgtc atattcggta acggtgtctg gcgtcagccc ggtcaccgtg
     6421 acgagggcgt agtgatgacc ctgtacggcg aacgtccgcg ctgagcagcc gagcacctcc
     6481 accgttgccg cgctttcggt ttgcacccag accaacgccg tggtgtctgg aacgtgacgt
     6541 agggcagggc cgagcaagag tgccaccgaa ccaccgtacg gttctgcatc gcggcgggag
     6601 tagtgggtcg atgacacgtc tacagcggca ctgcgtcccg cgcggtgtgc acgttgcgcg
     6661 cgatttcgtc gaggtgcgta tcccatgtgc cgcagcaccc accccacatg tcgaggtgcg
     6721 ggtactgccg cgcgaggcca cccatgagct cgccgaggtg tgcggggtcg cccgcctcga
     6781 ggtgcccgag cgtgcagagc gaaatcttgt ccatcatcgc ggcgttgggt cgcaggaccc
     6841 gcacgcgctc gaaccagctg ccccgctcga ttgcgggcat gaattcgagc ggatgcgagc
     6901 agttgatgcc gtagaacgcc ggccggtcat cacccgtctg cgcgtcgacg gtctcgatcg
     6961 cgtccttcag cgtcggccca gacaagagcc ggctggtgct gttgtcgagc gtgaacgaga
     7021 cggacagagg aagaccgact tgtgaagcgg cgcgcgataa tccgatgact tcgtcgacgc
     7081 tgttgaacgt catcgcctcg accaagtcga cgctcgcacg agccagcgtg gcgagctgct
     7141 cgctgtggta ctcctcggcc tcctgcgcag tgatcgtccg gttcaactcg tacgcgtcgc
     7201 cgcgcgggcc gacgatcccg gcgtacagca ccgccggcac ctgcccgcgg tacggctccg
     7261 cgacgtcgcg cagaaagtcg atcgctcgca tctgcatctg ggccagcgcc tcgccgcggt
     7321 agccaagcag cgacgcccag tccgggctcg cgcggtagtc gaggccgccg atgaccacgc
     7381 cgaagccatg ccgcgcggcg gtgtcgagat accggccgta cattccggac agctcggtca
     7441 tcgcgcgcgg gttgtcgagc agcgggaaca tcgcgaagtg cggcagctcg aagccgtact
     7501 tgtacaagat ctcggtttcc tggccaccct cggtcaggta gttgaggcct gccttctgct
     7561 tcggaaattc cttggccatg tcggttcccc cgtgctcctg tgaacccact gtgagcaacg
     7621 gccagcgtag ccgctgacca gaccgttcga aggggatccg cggggtcggc cggataagcc
     7681 cgaggcagca cgcagcgcat ccgcgttggc atcgatcgcg ttgattcaga cggcgttcat
     7741 ccactgttgg ttttgcggat cgccgcctac gtcccggtat gtcagcattg cgcggtgagg
     7801 gcacgccgta tcgcacttct cgtcggtgct gcaacagcag caagttgggc gccactgagc
     7861 gcagtcgttc cgtccgcgtc cgcgcagccg tgccccgacg tcgaggtggt attcgcgcgc
     7921 ggcacaaccg aagaccccgg ccttggccca acggggcagg cgttcgtcga ctcgttacgc
     7981 gcgcgagtcg gggctagatc gatggggata tatccggtcg actaccccgc caccgttgac
     8041 ttccccaccg ccctcgacgg catcagggac gcgagcgccc acatcgaatc caccgcggcg
     8101 aactgtccca acaccaagat ggtgctcggc ggattctccc aaggcgcagc ggtgatgggc
     8161 ttcgtgaccg cgaatgtgat acccgatggc gcacccgacg gtgtgccgaa cccgatgccg
     8221 cccgacgtgg ccaaccacgt cgccgcggtg gccctgttcg gaaagccgaa cgcccggttc
     8281 atgcgagcga tcaatcagcc acagatcgac gtcggcccgc agtatgtggc gaagaccatc
     8341 gacttgtgcg tacctgatga cttcgtgtgc tcaaccggac gggatttcaa cgcgcacacc
     8401 caatacgcgg acaccggaat ggttgacgag gcggcgacgt acaccgcgaa tcacgtcctg
     8461 gcagcgttgg gccctgctgc gccgccgcaa gcacctgctc ccgcaccgcg acaattgcct
     8521 gctcccgccg cgccaccgca agcacctgct cccgccgcgc caccgcaagc accgactccc
     8581 accgcaccgc cgcaagcaac tcctcccgcc gcaccgccgg acgcaactcc tcccgccccg
     8641 ccgccgttta acgcacttcg accgcttccc cccgggacac tgttgaggtg cgacaccgtc
     8701 tgtcacatca tcggtcccgc ttaacgaggc gatttcgcgt cgaagcggct ttgtagcgtt
     8761 agccgacgga tttacggacc ccaccgcgcg acgtccgact ctggtatgac tgagccagac
     8821 acaaccccgt agtgaggagt ccgcaccgag gtgcccaact ggtgcggtca gcggatgagg
     8881 gacttgtgag cgcgccgacg atcgacaccg ccatcgccgc cgagcaaccc cggagatccg
     8941 gcgtggccag gtggatccgc cgactggcgg tcccgatcat cgtcggctgg atagcgctca
     9001 tcgcgttcct gaacgtggcc gtgccacagc tcgaagtcgt cggcgagatg cggtcggtgt
     9061 cgatgtcgcc gaaagaggca ccatcggtgg ccgcgatgat gcgcatcggc caggtcttcg
     9121 aggagttcga ttccgacagc tcggccatga tcgtcctgga gggcgaggcg cccctcggtg
     9181 aggacgccca ccggttctac gacgacatga tcggcaagct gcgggccgac accaagcacg
     9241 tccagcatat tcaggacttc tggggcgacc ccctgacacg ggccggagcg cagagcgagg
     9301 acggcaaggc cgcgtacgtt caggtgtacc tcgccggcaa tatgggtgaa gctctcggca
     9361 acgagtcagt cagccagatg caaaagctcg tcgagggatt gtcacccccg ccgggcgtca
     9421 aggtcttcgt gaccggagga ccggcactac aggccgacca agaggccgcc ggcaataaga
     9481 gcgtccgaat catcgagttc gtcacggtcg ccgtcatcgt cctgatgctg ctgttcttct
     9541 atcggtcgat cgtcacagtc gtgctcgtct tggtaatgct ggtgctatcg ctgtcggtga
     9601 cccgtggtgt ggtggcattc ctgggctact acaacctgat cgggttgtcg acgttcgcga
     9661 ctcagctctt ggtgaccctc gcgatcgccg ccacgaccga ctatgcgatc ttcctgatcg
     9721 ggcgctacca ggaggcccgc acgatcgggg aagaccgaga gtcggccttc tacaccatgt
     9781 tccgcggcac tgcccacgtc gtgctgggct cgggcatgac gatcgccggt gcaacgttct
     9841 gcctctcgtt cacccggctg ccctacttcc agacactggg tgtcccactg gccg
//
LOCUS       DASRJA010000216         7608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3528122,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000216 DASRJA010000000
VERSION     DASRJA010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3528122"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFC01_09660"
     CDS             complement(<1..226)
                     /locus_tag="VFC01_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79935.1"
                     /translation="MVMEANRTEGAEPPAMPAVEQLRAKLRVARPGLRFSEVMTGMIT
                     MGIADPAQGYKNDAAIAMRLHATVTIPNLPA"
     gene            complement(220..1317)
                     /locus_tag="VFC01_09665"
     CDS             complement(220..1317)
                     /locus_tag="VFC01_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79936.1"
                     /translation="MAIAAPPSTNGQTPSASDPCEPIAPDRFETIEFAARDGFTLNFK
                     HLPPASGKTTKPLLMVAGTGTRANLWNPPTDNLLKQLTASGFDLWMLNWRSSIDLPAN
                     NYTLDDAAVLDMPRAVQEVRERTGEDTIKALIHCQGSCAFMMAITAGLLPDVSMVISN
                     SSALHPVMPWQARMKLPLAVAFLNREKVNAINPQWGLHAPRLLPKILDWYVKATHDEC
                     DNAVCNHTSFMYGYGHPTLWRHENLNDATHEWIKGEFGQVPMTYYGHIADSSAAGEMV
                     STGKYAELPPLFTAEAPLTDARFVFMTGDRNQTFLPIGMGRTFEYFERYAPGRHTFQK
                     FAGYGHLDVFLGESSHRDVFPFILDELSKPW"
     gene            complement(1348..3990)
                     /locus_tag="VFC01_09670"
     CDS             complement(1348..3990)
                     /locus_tag="VFC01_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZN79937.1"
                     /translation="MAIEWRTVPRGQSLGLLESTLKSKNRLGAVLAGSAGVGKTVLAR
                     HAADRFAQRHGGSAVQWLAGTASARQIPFGAFSHLVDVAGVGDSPTLLRSARASLLQH
                     GGQGLLLAIDDAHHLDNLSATLVHQLALTGSARFIITVRAGEQAPDAIVSLWKDRLLD
                     RLDIEPFDAAQTKSLLEAVLGGPMETSSADAVFTASQGNPLYLRHLAENAIDSGALRQ
                     VDGIWQLRGHIGLSAELSALIGRHLEGLPDTVTSVLEYLAVEEPLGLRDLSELAGREA
                     LEEAEDLRAIEVVKRGDDLVVHSAHPLYAEQVRASLGQIATRRLRTQLVAQLSSKPIE
                     HVSARLRLAALAIESDTPPPVDDVVASSWEAMRLGDLMLGERLAQSALDRSGGLTARL
                     PLAHALSWLGRGPDADQVLAPVDPDSLSEWDLTAWTLPKAANQFWMLDQPEAAVDFLY
                     AMRDRVSEPAAVATIDALVATFAMNSGDPRRAVEIAADVLDSPASQDLAIAWAAATAT
                     LSSARLGRFDDVGPLAERGLAATHPGLLRFTIGLGEITTLSMTKSVMHAQRLARHFME
                     FSELQQPGRAIGEILLARTLIATGEFDLATSLLRQSAAALSETGYSWGPLALMYLAQA
                     LGQQGDAMGSAEILARAESRHSMRSELYAPELALGRAWTLAAARNMIGAISAARDAAR
                     IAERSGQLAIALQALHDAVRLGDVTAADAIARVSSSLDCVAGRAALAHGRALAAGNGK
                     ALTAVAAELADLGMKCAAADAAAQAAKVFAARHERKGEVAAMAQAAELAGGASTPALD
                     QVLNPLPLTGRELEIAIMVSEGMTNKAIAERLSVSVRTVEGHIYRACTKLDVADRTLL
                     AEVVASAKASSRLRKTSPRSPSTL"
     gene            4188..5585
                     /locus_tag="VFC01_09675"
     CDS             4188..5585
                     /locus_tag="VFC01_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79938.1"
                     /translation="MGLGRHIERASAVALALGVVIAVSALPVAAEGMSASDQMPASAG
                     WAGVISALLVAVGRRPAVPGRPDDRPPAPVAPVRTVAGIGPVDDIALSPDGRYAYVAA
                     LRSDRINGTEKWTVKVVDTAADVVIKTIDDLSEVIPTSLAASPDASRLYVADRGAAAI
                     RVVDIQEGSPTRNRIVDTLRLDRSLAVCYGTPTAIALSPKGSRLYALMTDHAVLIVDP
                     ETRVLAGTVSGGDSVAMFSAGEHIYVCADDRLGNTNVAAGVVILEADTGRELGSFGIN
                     DAPAFIRQPILAGSSGQLYLDIDDLDGRLEVVDTSTLKVVSRITIEASADRQLRSIPS
                     AAALSPDGRCLYVANQGVRLSSDEATEVGWVSVVDTRLNKVVDDIQLPQSGIDESQGA
                     NPQFIAISPAGDRLVVVNEQAIASIVDVASRSVVSTVTFGEPTDQPRVVVVNPARNAV
                     YVGTSGEAGLTVISL"
     gene            6258..6950
                     /locus_tag="VFC01_09680"
     CDS             6258..6950
                     /locus_tag="VFC01_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZN79939.1"
                     /translation="MSQSTIVGKSQFLTAATVLSTIGLLVIPAPAQARPTFPLAPACN
                     DYKFTGDFALQQSNGARVTFFSNSQAASGPADATGTGAGTMHGTVSGYVRGIFVDFTI
                     RWNNGPRGHYRGQIDESDFAHGDTVDEANPESRATWDSTVPFGCMTPPPAPAPAPAPA
                     PAPASAPAPAPAPAKAAVTGDVDVYDAPGGNGNVIGMLRQGQQVQLQGACNKDDWCQV
                     VGQGWVWGHLQF"
     gene            complement(6978..>7608)
                     /locus_tag="VFC01_09685"
     CDS             complement(6978..>7608)
                     /locus_tag="VFC01_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN79940.1"
                     /translation="IAGYHPQTDGELLVDGEATHFNSPKDALAKGIATVYQNLAVVPL
                     MPVWRNFFLGQEIRKKTFPFPLDSNAMRATTLTELSKMGIDLPDVDVPIGSLSGGQRQ
                     CVAIARAVFFGARVLILDEPTAALGVKQSGVVLKYITAAKEAGFGVVFITHNPHHAHM
                     VGDHFVLLNRGRQKLDCTYDDITLEHLTQEMAGGDELEALSHELRGGKG"
BASE COUNT         1343 a         2459 c         2426 g         1380 t
ORIGIN      
        1 acgcaggcag gttcgggatg gtgacggtag catgtagccg catcgcgatc gcggcgtcat
       61 tcttgtaccc ctgcgcggga tcggcgatgc ccatggtgat catcccggtc atcacttcgc
      121 tgaagcgcaa acccgggcgg gccacccgca acttcgcgcg gagctgctcg acggccggca
      181 tggcgggcgg ctcggcgccc tctgtgcgat tcgcttccat caccatggct tgctcaattc
      241 atcgaggatg aacgggaaga cgtcgcggtg cgaggactcc cccaagaaca cgtcgagatg
      301 cccgtagccg gcgaacttct ggaaggtgtg cctaccgggc gcgtaccgct cgaagtactc
      361 gaaggtgcgt cccatcccga tcggcaggaa ggtctggttc cggtccccgg tcatgaatac
      421 gaagcgggca tccgtcagtg gcgcctcggc ggtgaacagc ggcggcagct cggcgtattt
      481 ccctgtcgat accatctcgc ccgctgccga tgagtccgcg atgtgcccgt agtacgtcat
      541 cggtacctgg ccgaattcac ccttgatcca ttcgtgggtg gcgtcgttga gattctcgtg
      601 gcgccacagg gtggggtgtc cgtacccgta catgaagctg gtgtgattgc acaccgcgtt
      661 atcgcattcg tcatgggtgg ccttgacgta ccagtcgaga atcttgggca gcaggcgcgg
      721 cgcatgcaga ccccactgag ggttgatggc gttgaccttc tcgcggttca agaacgcaac
      781 ggcgagcggc agtttcatcc gcgcctgcca cggcatgacc gggtgcagcg ccgaggagtt
      841 cgagatcacc atcgacacgt cgggcagcag tccggcggtg atagccatca tgaacgcaca
      901 cgaaccctgg cagtgaatca gcgccttgat cgtgtcttca ccggtccgct cgcgcacctc
      961 ttggaccgct cgcggcatgt cgagcaccgc cgcatcgtcg agcgtgtagt tgttcgcggg
     1021 caggtcgatg ctggagcgcc agttgagcat ccacaggtca aacccgctcg cggtcaactg
     1081 ctttagcagg ttgtcggtcg gcgggttcca gaggttggcg cgggtgcctg tcccggcgac
     1141 catcaacagc ggcttggttg tcttccccga tgccggcggc aggtgcttga agttcagggt
     1201 gaagccgtct cgcgccgcga actcgatggt ttcgaagcga tctggggcaa tcggctcgca
     1261 cgggtcgctg gccgacgggg tctgcccatt ggtcgacggg ggtgctgcga tagccatctg
     1321 aactcctgcg cgtcgcgggt taccgggcta cagcgtgctc ggcgatcgcg gggacgtctt
     1381 gcgtagtctg ctactcgctt ttgcagaggc gacgacttcg gccagcaggg tgcggtcggc
     1441 gacgtcgagt ttcgtgcatg cgcggtagat gtgtccctcg accgtgcgca ccgagacgga
     1501 cagccgctcg gcgatggcct tgttggtcat cccctcggac accatgatcg cgatctcgag
     1561 ctcccgtccg gtcagcggca gcgggttgag cacctgatcg agtgcgggtg tcgacgcgcc
     1621 acccgcgagc tcggcggctt gagccatggc cgccacttca cccttgcgct catggcgggc
     1681 ggcgaacacc ttggccgcct gggcggcggc atcggcggcg gcgcacttca tgccgagatc
     1741 ggcgagttcc gcggcgacgg cggtcagcgc cttgccgttg cctgcggcca gcgcacggcc
     1801 gtgcgccagt gcggctctgc cggcgacgca gtccaggctg ctggagacgc gtgcaatggc
     1861 gtcggctgcg gtgacatcgc ccagccggac cgcgtcgtgc aaggcctgca gcgcgattgc
     1921 cagttgcccc gaccgttcgg cgatccgggc cgcgtcgcgg gcagcgctga tggcgccgat
     1981 catgttgcgg gcggcggcca gcgtccaagc acgtccgagg gccagctcgg gtgcatacaa
     2041 ctccgacctc atgctgtgcc gagattcggc gcgcgccaat atctctgctg aacccatcgc
     2101 atcgccctgt tgaccaagtg cctgcgccag gtacatcagt gcgagtgggc cccaggaata
     2161 accggtctcc gacagcgccg cagcggattg acgcagcagc gacgtcgcca ggtcgaactc
     2221 ccccgtcgcg atcaaggttc tggcgagcag gatttcgccg atggctcggc ccggttgctg
     2281 cagttcggaa aactccatga agtggcgtgc cagccgttgc gcgtgcatca cgctcttggt
     2341 catcgaaagc gtggtgattt cgccgagacc gatggtgaac cgcagcagcc cgggatgtgt
     2401 cgccgccagg ccgcgttcgg ccagcgggcc gacatcgtcg aatctgccga ggcgcgcgct
     2461 cgacaacgtc gcggtagctg cggcccatgc gatcgcaagg tcttgggaag caggcgaatc
     2521 cagaacgtcg gcggcgatct ccacggcgcg ccgtggatcg cctgagttca tcgcgaaggt
     2581 ggccaccaat gcatcgatcg tcgcgactgc cgcaggctcc gatacccgat ctcgcatcgc
     2641 gtacaggaaa tcgacggcgg cctccggctg atcaagcatc caaaattggt tggccgcctt
     2701 cggcagtgtc cacgcggtga gatcccactc cgaaaggctg tccgggtcga ccggggccag
     2761 cacttggtca gcgtcgggcc cgcggcccag ccaagacagc gcatgcgcca acgggagtct
     2821 ggcggtcaag ccgcccgagc ggtcaagggc gctctgggcc agacgctccc cgagcatcag
     2881 atcgcccagc cgcatcgcct cccacgacga ggcgacgaca tcgtcgactg gcggcggggt
     2941 atcgctctcg atagccagcg ccgcgagcct caacctggcg ctgacatgct cgatcggttt
     3001 cgacgacagt tgcgcgacca gctgggtgcg caacctcctg gtcgcgatct ggccgagcga
     3061 ggcacgtacc tgctcggcgt agagcggatg tgcggagtgc acgacgaggt cgtcgccgcg
     3121 cttgacgacc tcgatcgcgc gaaggtcctc ggcttcctca agcgcctcac ggcccgccag
     3181 ctcggacaga tcccgcaagc cgagcggctc ctccacggcc aggtattcga ggaccgatgt
     3241 caccgtgtcc ggcaggccct cgagatgccg accgatcaac gcggaaagtt cggccgacag
     3301 cccgatatgc ccgcgcaatt gccatatgcc atcgacttgg cgtaatgcgc ccgagtcaat
     3361 cgcattttcc gctagatgac gcaaatacag tggatttcct tggctcgcgg taaataccgc
     3421 atcggcgctc gaggtctcca tcggcccgcc aaggacggct tccagcaaac tcttagtctg
     3481 cgcggcgtcg aaaggctcga tgtcgagccg gtcgagcagc cgatccttcc acagcgagac
     3541 gatcgcatcc ggggcctgct cgccggcccg cacagtgatg atgaatctcg ccgacccggt
     3601 caacgcgagc tggtgcacca gtgtcgcgga gagattgtcc aggtgatgcg cgtcatcgat
     3661 ggctagaagc agtccttgcc cgccatgctg cagcagggat gcgcgggccg accgcaacaa
     3721 cgtcggggag tcgccgacgc cagcgacgtc gacgaggtga ctgaacgcgc cgaacgggat
     3781 ctgacgggcc gacgcggtgc ccgccagcca ctggacggcc gagccgccgt gccgctgagc
     3841 aaatctgtcg gcggcatgcc gagcgagcac ggttttgccg acgcccgccg agccggcaag
     3901 caccgcgccc aagcggttct tgcttttcag cgtcgactcc aaaaggccca gcgactgccc
     3961 gcgcgggact gtgcgccatt caatggccat tcgcgaatcc tatgacgacc gtgccgtttt
     4021 ctcattgcat acgagcaaac tgtggtcggc atcggtgccg tgcgtcgacc ctgcagcacc
     4081 taaaaacagg tagtgcacta cgcgtgtttt tgggggccac ggcttcctac cctgcggagg
     4141 cggagcaaac tccgcgaacc tccgggggga ggacaggaga tgaacggttg ggcctgggaa
     4201 ggcacatcga gcgggcgagc gcggtggcgc tcgcgttggg ggttgtgatc gcggtgtcgg
     4261 cactgcctgt ggccgctgag gggatgtccg cgtcggacca gatgcctgcg tcggcaggct
     4321 gggccggcgt catctcggcc ctgcttgtcg cggtgggacg tcgacccgcg gttcccgggc
     4381 gccctgacga tcgacccccg gcgccagtcg caccggtgcg gacggtcgcc gggatcggcc
     4441 cggtggacga tatcgcgcta tcgccggacg ggcgatacgc atatgttgcg gcattgcgct
     4501 cggatcgcat caacggcacg gaaaagtgga cggtcaaggt tgtcgatacc gcggccgacg
     4561 tggtgatcaa gaccatcgac gacctttccg aggtcatccc gacaagcctt gcggccagtc
     4621 ccgatgcgtc gcggctctac gtcgccgacc gcggtgccgc cgctatccgc gtcgtcgaca
     4681 ttcaggaggg cagcccaacc cgaaaccgga tcgtcgatac gcttcgcctc gacagatcgt
     4741 tagccgtctg ctatggaaca ccgacggcaa ttgcactgag ccccaaagga tcacgactgt
     4801 acgcgctgat gacggatcac gcggtgctca tcgtggaccc cgagacacgt gtactagccg
     4861 gaaccgtgtc cggcggtgat tcggtagcga tgttttcggc cggtgagcac atctatgtct
     4921 gcgccgacga tcgcctcggc aacaccaatg tcgccgcagg agtcgtgatc ctcgaggccg
     4981 acaccggccg agaactcggt tcgttcggca ttaacgacgc gcccgcattc attcgccaac
     5041 cgatcctggc cggtagtagc ggtcagttgt acctcgacat cgacgacctc gacggccgct
     5101 tggaagtggt cgacacgtcg acgttaaagg tggtgtcgcg catcactatt gaggcgtcgg
     5161 cggatcgcca actacgtagt atcccgagcg ccgcagccct gagccccgat ggccgatgtc
     5221 tatacgtggc caaccaaggc gtgcgcctgt catcagacga ggcgaccgag gtcggctggg
     5281 tttcggtggt cgacacgcga ctcaacaagg ttgtcgacga catacagctg ccgcagtcag
     5341 gcatcgacga atcgcagggc gccaacccgc aattcatcgc gatcagcccc gcgggcgatc
     5401 gtctggtcgt ggtcaatgag caggcaatcg cgtcgatcgt cgacgtcgcc tccaggtccg
     5461 tcgtcagcac cgtgaccttt ggggaaccaa ccgaccaacc gagagtggtc gtcgtcaacc
     5521 cagcccgcaa cgcggtgtac gtcgggacgt ccggcgaagc cggactaacc gtgatttcgc
     5581 tgtagcggtc gccgtcgcgg cgggcgtgcg tcgctctgcg gaggtcggat gtcgctttcg
     5641 tcgagcgcgc ggcgttcgtg ccaccgcagc cgcagcagca gcaagtggcg gtgcgcacgg
     5701 aaccccgctg acagcgggtc acgccagacc ggcgtgtgag ccttcgcttc gggttctgtg
     5761 tccacgtcgc ccattgtgcc gtgtcaatcg catacgggcc tcgctggcac gcttccgccc
     5821 aggtgaacgg tcgcataacg tttgtgacgg agcagacccg caggccgggc gcccggcggc
     5881 ccccgccggc cacaaaggcg gattcgactt tgcgatcagt gacaataaag gttcacattt
     5941 tgctgatcta attgcaatga caacgatcta ttttctgcgg gcgcaacgat tttctattgt
     6001 tttggtccgc caacaatcga attagagaaa ttctgcagaa gactcacaga ctatttcaac
     6061 tcggcctaca atccgtcatg tgggtcctga aatcccaggc tagggaggtt tattacggcc
     6121 atcccttatc aagttcacgc ttaatccacc gtaccgaaat cgatcgaaac accccttttc
     6181 gccctggggt cctgcggtga atatcgtgcg ctcgatgggg taactgactc cattaaccac
     6241 ccgaacggaa aattccgatg tctcaatcaa ccatcgtcgg caagtctcaa ttcctcaccg
     6301 ctgcaacagt gttaagcacg atcggattac tcgtaatccc tgcgcccgcg caggcgcgcc
     6361 cgacatttcc ccttgcccct gcctgcaacg actacaagtt cactggggat ttcgcactcc
     6421 aacagtccaa cggcgccagg gtgaccttct tctcgaactc gcaggccgcc tccggacctg
     6481 ctgacgccac aggcacaggc gctggcacga tgcacgggac cgtcagtggc tacgttcgcg
     6541 gcatcttcgt cgacttcacc atccgctgga acaacggtcc cagaggccac tacaggggcc
     6601 agatagacga gtccgacttc gcgcacggtg acaccgtcga cgaggcgaat cccgagtcca
     6661 gggcgacctg ggactccacc gttccgttcg ggtgtatgac gccgccgcct gctcccgctc
     6721 cagcgcctgc tccggctccg gctccggctt ccgccccggc gcccgctcct gccccggcaa
     6781 aggcagcagt caccggtgac gtggatgttt acgacgcccc cggcggaaac ggaaacgtaa
     6841 tcggcatgct gcggcaaggc cagcaggttc agcttcaagg agcctgcaat aaagacgact
     6901 ggtgtcaggt ggttggccaa ggctgggttt ggggacacct gcagttctga gtggccacag
     6961 cggttggcta ccccgcgcta acccttcccg ccgcgcaatt cgtgcgacaa cgcctccagc
     7021 tcgtcgccgc ctgccatctc ctgggtcagg tgttccaggg tgatgtcgtc atacgtgcag
     7081 tccagcttct gacgaccgcg gttgagcagc acgaagtggt caccgaccat gtgcgcgtgg
     7141 tgcgggttgt gcgtgatgaa caccacaccg aagccggctt ccttcgcggc ggtgatgtac
     7201 ttcagcacca cgcccgactg cttgacgccg agcgcggccg tcggctcgtc gagaatcagc
     7261 acccgcgcac cgaagaacac cgcacgcgcg atagcgacgc attgccgctg accgcccgat
     7321 agcgagccga tcggcacgtc gacgtcgggc aggtcgatgc ccatcttcga cagctcggtc
     7381 aatgtggtgg cccgcatcgc gttggaatcc aacgggaacg ggaaagtctt cttacggatc
     7441 tcctggccca ggaagaagtt gcgccacacc ggcatcaacg gcacgacagc gaggttctga
     7501 tacaccgtcg caatgccctt ggccaatgcg tcttttggcg aattgaagtg cgtggcttcg
     7561 ccgtcgacca gaagttcgcc gtcggtctgc gggtgatatc cggcgatg
//
LOCUS       DASRJA010000217         5946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1834634,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000217 DASRJA010000000
VERSION     DASRJA010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834634"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFC01_09690"
     CDS             <1..496
                     /locus_tag="VFC01_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_178027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZN79941.1"
                     /translation="RPVNRAPSDVERAATANTVGADLMISLRCAMQASPAANGVASFH
                     FGNSHGSVSTIGRMLSDFIQREVVARTGLRDCRTHGRTWDLLRLTRMPSVQVDVGYIS
                     NPRDRAMLVDASARDSIAEGILAAVKRLYLLGKNDRPTGTFTFAELLAHELSVEQAGR
                     VSPT"
     gene            complement(501..1268)
                     /locus_tag="VFC01_09695"
     CDS             complement(501..1268)
                     /locus_tag="VFC01_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HZN79942.1"
                     /translation="MAARVMPLRLESFEQLPKHARRCVYWEVDPSTLRPDDHLSDPEF
                     EKEAWLSMVMLEWGSCGQMAVHCPDSEAMASPDDDPYLPLSAGDSPCLGYAFYAPPGA
                     VPRARQFPTGPVSADAVLLTSLGVEAGHDAEGLSRHLIASVVGDLVRRGVRALEAFGH
                     TGEVTELLDPGLVSPELRPVVDVLGDCSVDQCVLDADLLQDAGFVVVTHHRYFPRLRL
                     ELEQGLGWKADVEAALERLLESAQLQQPVGAGASPCV"
     gene            complement(1486..2472)
                     /locus_tag="VFC01_09700"
     CDS             complement(1486..2472)
                     /locus_tag="VFC01_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HZN79943.1"
                     /translation="MTQPTRKRSGLGRGLASLIPTGPADGEAPGSFGPRMGDAAADVV
                     LGGPPAIDGADVGAVYREIDPSSIDPNPRQPRQVFDEEALAELVHSIREFGLMQPIVV
                     RALPATPSTPPRYQLVMGERRWRAAQQAGLATIPAIVRETADDSMLRDALLENIHRVQ
                     LNPLEEAAAYQQLLDEFGVTHDELAARIGRSRPLITNMIRLLRLPIAVQRRVAAGVLS
                     AGHARALLSLDGGPEQQEELAARIVAEGLSVRATEEAVTLANRDTTATPSAPRRKPIH
                     MPGLQDVAERLSTAFDTRVTVSLGKRKGKIVVEFGSVEDLQRIVDLMNSSNT"
     gene            complement(2533..3531)
                     /locus_tag="VFC01_09705"
     CDS             complement(2533..3531)
                     /locus_tag="VFC01_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZN79944.1"
                     /translation="MSPVPEGVPTPGAAPPQTTQRPDVSRETWESAPQQSWADEAGLD
                     TPIGAEAERAVRLLHAAAKGGLPRPTHQRVFTIANQKGGVGKTTTAVNVAAALALQGL
                     HVLVIDLDPQGNASTALGIEHREGTPSSYEVLIGEIPLQTAIQRSPHSERLFCVPATI
                     DLAGAEIELVSMVAREGRLRTALAGLQNHDFDYVFIDCPPSLGLLTINALVAAPEVLI
                     PIQCEYYALEGVGQLLRNIEMVKAHLNPALNVTTVILTMYDGRTKLADQVAADVRAHF
                     GDKVLRTVIPRSVKVSEAPGYGMTILDYDPGSRGAMSYLDASRELAERGTPEGQQR"
     gene            complement(3528..4229)
                     /gene="rsmG"
                     /locus_tag="VFC01_09710"
     CDS             complement(3528..4229)
                     /gene="rsmG"
                     /locus_tag="VFC01_09710"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HZN79945.1"
                     /translation="MFHVKHVEVSAAPAAAAALFGSRLDRAQRYAQILAGTGVERGLL
                     GPREVGRLWDRHILNSAAVAELFDSNDRIADIGSGAGLPGIPLALARPDLRLTLIEPL
                     LRRSEFLREVVDDLGLEITIVRGRAEDRSVRQQVGEMDAVVSRAVASLDKLTKWSLPL
                     LRPDGRMVAIKGERAEEEIREHRRVMTSLGAVDVRVKRCGADYLDPPVTAVVARREAT
                     DPVANRSRRTGRRKG"
     gene            complement(4291..4845)
                     /locus_tag="VFC01_09715"
     CDS             complement(4291..4845)
                     /locus_tag="VFC01_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HZN79946.1"
                     /translation="MTDAETTERSEELAVEENGTDSAPATGDEDLEERLVAEGEIAGD
                     YLEELLDLLDFDGDIDLDVEGDRAVVSIDGGGDLNKLVGRKGEVLDALQELTRLAVHQ
                     KTGERSRLMLDIARWRRRRREELAALGDKVARRVLESGEREELTPMTPFERKIVHDAV
                     AAVDGVHSESEGVEPSRRVVVLLD"
     gene            complement(4882..>5946)
                     /gene="yidC"
                     /locus_tag="VFC01_09720"
     CDS             complement(4882..>5946)
                     /gene="yidC"
                     /locus_tag="VFC01_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZN79947.1"
                     /translation="YPVSAIMWVWYKAFAFILGPSNFFAWGLSVMFLVFTLRAILYKP
                     FVRQIRTTRQMQELQPQIKALQKKYGKDRQRMALEMQKLQREHGFNPLMGCLPIFAQV
                     PVFLGLYHVLRSFNRTQGGFGQPQLSPELNRSLGNYVFSATDVGHFLDANLFGAPLGA
                     TMIQNQGLGAFTEFSRPAVIAVGVPLMIAAGIATYFNSRASVARQSPEAAANPQTAMM
                     NKLALYVFPLGVVVGGPFLPIAIILYWFANNIWTFAQQHYVFGNIAKEEEAKKLEAQE
                     RRAANAPPPGAKPNRARKAQAAPVTDDVSDAVVDGKATDGEAGSNSAGKSSDGKAAGN
                     AAPRPGATPRPGARPKKRKR"
BASE COUNT          939 a         2086 c         1884 g         1037 t
ORIGIN      
        1 gcggccggtc aatcgagcgc cgtccgacgt tgaacgcgcc gcgaccgcca acaccgtcgg
       61 cgcggacctg atgatcagcc tgcgctgcgc catgcaggcc agccctgcgg ccaacggtgt
      121 ggcttccttc cacttcggca attcgcacgg gtcggtatcc accatcggcc ggatgttgtc
      181 cgatttcatt cagcgagaag tggtggcgcg caccggatta cgcgattgcc gcacacacgg
      241 caggacgtgg gacctgctgc ggctgacccg gatgcccagt gtgcaggtcg acgtcggcta
      301 catcagcaac ccgcgcgacc gcgccatgct ggtggacgcc tcggcccgcg actcgatcgc
      361 cgaaggcatc ctggccgccg tcaagcggct gtatctgctc ggcaagaacg accggccaac
      421 gggcacattc actttcgccg agctgctggc gcacgaactg tcggtcgagc aggccggccg
      481 cgtcagcccg acctgatccc ctagacgcag gggctcgcac ccgcaccgac gggctgctgc
      541 agctgcgcgc tttccagcag gcgctccaac gccgcctcga catcggcttt ccagcccaac
      601 ccctgctcga gttcgagccg cagccgcggg aagtagcggt ggtgggtcac aacgacaaaa
      661 ccggcgtcct gcagcaggtc ggcgtccagg acgcactggt ccaccgagca gtcccccagc
      721 acatcgacga ccggccgcaa ctccggtgac accagtccag gatccagcag ctcggtcact
      781 tcgccggtat gcccgaatgc ttcgagggct cgcacgccgc ggcggacgag atcaccgacc
      841 actgacgcga tgaggtggcg ggacaacccc tcggcgtcat ggcccgcttc gacacccagc
      901 gaggtgagca gcacggcgtc ggcgctcacc ggtccagtgg gaaactgccg cgcacgtggc
      961 accgctcccg gcggcgcgta aaacgcgtac cccaggcagg gactgtcccc tgcactgagt
     1021 ggcaggtagg ggtcatcgtc ggggctggcc atcgcctcgc tgtccgggca gtgcaccgcc
     1081 atctggccgc acgatcccca ctcgagcatg accatggaca gccaggcttc cttctcgaat
     1141 tcggggtcgc tgaggtgatc atcggggcgc agggtcgacg gatccacctc ccaatacacg
     1201 caacggcggg cgtgcttggg cagctgctcg aacgattcga gccgcagagg catgactcgc
     1261 gctgccacta aactccccga gttgtatgcg gtgctgaact gcacggctgc cactccagga
     1321 tagaagcccg cgccgccgcg gacccatttt cgcccggttc gctcaccggc cgcagccctc
     1381 ggctcgaaac ctattgctac acatggcttt tctttcgtga gaggcgcgcg gacggccggg
     1441 tgaggaagtt tgtgtcacag tgacgtaatt tcgccgtgtc gtaggtcagg tgtttgacga
     1501 attcataagg tcgactatcc gttgtaggtc ctcgacggat ccgaactcga ccacgatctt
     1561 gcccttgcgt ttgccaaggc tgaccgtcac gcgcgtgtcg aacgccgtgg atagccgctc
     1621 agcaacatcc tgtaggcccg gcatgtgaat cggcttgcgg cgcggtgccg agggtgtcgc
     1681 ggtggtgtcg cgattcgcca gcgtgaccgc ctcctcggtc gcgcgtaccg acaggccttc
     1741 cgcgacgatg cgcgccgcca gctcctcctg ctgctcgggg ccgccatcaa gggagagcag
     1801 cgcgcgggcg tgtcctgccg acagcacccc ggcggccacc cgccgctgca ccgcgatcgg
     1861 cagccgaagc agccggatca tgttggtgat gagcgggcgc gaccggccga tgcgggcggc
     1921 gagttcgtcg tgggtgaccc cgaactcgtc gagcaactgt tgataggcgg ccgcttcttc
     1981 caacgggttc agctggacgc gatggatgtt ctccaacagg gcgtcgcgca gcatgctgtc
     2041 atcggcggtc tccctcacga tcgcggggat ggtggccagc ccggcctgtt gggccgcgcg
     2101 ccagcgccgc tcccccatga cgagctgata ccgcggcggt gttgaaggcg tcgcgggcag
     2161 cgcccgcacg acgatcggct gcatgagacc gaactcgcgg atggagtgaa cgagctcggc
     2221 gagcgcttcc tcgtcgaaaa cctggcgggg ctgccggggg ttcgggtcga tcgacgacgg
     2281 gtcgatttcg cggtagacag caccaacgtc ggcgccgtcg atagccggcg gcccacccag
     2341 caccacgtcg gcggccgcgt cacccatccg cggcccgaag gagccgggtg cctcgccgtc
     2401 cgcgggccca gtggggatga gggatgcgag tccccggccg aggccacttc gcttacgtgt
     2461 cggctgggtc atcgctgctc tctcctgtcg gcgatgcggc tcatcgctgt tgtccgctct
     2521 tcgcgcaagc gctcatcgct gttgtccctc aggcgtgcct cgctcggcca gttcacgact
     2581 ggcgtcgaga tagctcatcg cgccccgcga gccggggtcg taatcgagga tcgtcatgcc
     2641 gtacccgggc gcctcggaga ccttgacgct gcgcgggatg acggtccgca gtaccttgtc
     2701 gccgaaatgt gcccgcacat cggcggccac ctggtcggcc agcttggtgc ggccgtcgta
     2761 catcgtcagg atcacggtgg tgacattgag cgccggattg aggtgggcct tgaccatctc
     2821 gatgttgcgc agcagctggc ccaccccttc gagcgcgtag tactcgcact ggatcgggat
     2881 caacacctcg ggcgcggcga cgagcgcgtt gatggtcagc aagccaagcg acggcgggca
     2941 gtcgatgaag acgtaatcga agtcgtggtt ctgcagcccg gcgagcgccg tgcgcagccg
     3001 tccctcgcgg gcgaccatgc tgaccaattc gatctcggcg ccggccaggt cgatcgtcgc
     3061 cggcacacag aagagtcgct cgctgtgcgg gctccgctga attgccgtct gcagcgggat
     3121 ctcaccaatc agcacctcgt aggaggacgg cgtgccctcg cggtgctcga tcccgagtgc
     3181 ggtgctggcg ttgccctgcg gatcgaggtc gatcacgagc acgtgcagtc cctgcagcgc
     3241 gagcgcggcc gccacgttga cggcggtcgt cgtcttaccg acgccgccct tctggttggc
     3301 gatcgtgaac acgcgctgat gcgtcgggcg cggcagcccg cccttggcgg cggcgtgcag
     3361 caggcgcaca gcgcgctcgg cctcggcccc aatcggggtg tccaggcccg cttcgtcggc
     3421 ccacgactgc tgcggggccg actcccatgt ttcacgtgaa acatccgggc gctgggtcgt
     3481 ctggggaggc gcagcacccg gcgtcggaac gccctcgggg accggactca tcctttcctc
     3541 ctgcccgttc gtcgggaccg gttcgctacc ggatccgttg cttcgcgccg cgccacgacc
     3601 gcggtgacgg gtggatccaa gtagtccgcg ccacatctct tcacccttac atcgactgca
     3661 ccgagcgacg tcatcacacg ccggtgctcc cggatctctt cctccgcacg ctcacccttg
     3721 atcgccacca tccgtccatc cggacgtagc agcggcaggc tccacttcgt cagtttgtcc
     3781 aacgacgcca ccgccctgga gacaaccgcg tccatctccc ccacttgctg tcgcacggac
     3841 cgatcctcgg cccgtccccg aacgatcgtg atctcgagac cgagatcgtc gaccacctcg
     3901 cgcagaaact cgctgcggcg aaggagtggt tcgatgagcg tcagccgaag gtcgggccgg
     3961 gccaacgcca acggtatccc gggcaggccg gctccactac ctatgtcggc gatccggtcg
     4021 ttggaatcga acagttccgc gaccgccgcg ctatttaata tgtggcgatc ccacagccgg
     4081 cctacttcac gcggcccgag gagcccgcgt tccactcccg tgccggccaa gatttgggcg
     4141 tacctctgcg cgcggtccag ccgtgatccg aagagggccg cagcggctgc cggcgccgcg
     4201 gacacctcca catgtttcac gtgaaacatc ctccgcatct cgcgggccgg gaacggatgg
     4261 ccgcggcagg aactacatcc gtgtaactct tcagtcgagc agaacgacga cgcgccgtga
     4321 cggctcgacg ccctcgctct cgctgtgcac tccgtccacg gccgcaaccg cgtcgtgaac
     4381 gatcttccgc tcgaacggcg tcatcggcgt cagctcttcc cgctctcccg actccaacac
     4441 ccgtcgcgcc accttgtcgc ccagcgcggc caactcctcg cgccgccgcc ggcgccaccg
     4501 cgcgatgtcg agcatcagcc ggctgcgttc accggtcttc tgatgcacgg ccaaccgggt
     4561 cagctcctga agcgcgtcga gcacttcacc cttgcgaccc accaacttgt tcaggtctcc
     4621 gccaccgtcg atgctcacca cggcgcggtc gccctcgacg tcgagatcga tgtcgccgtc
     4681 gaagtccaac aggtccaaca gctcctcgag gtagtcgccg gcgatttcac cctcggcaac
     4741 caaccgctcc tccagatcct catcgcccgt cgcgggtgcg ctgtcggtgc cgttctcctc
     4801 gaccgccaac tcctcgctgc gttccgtcgt ttcggcgtcc gtcatgtgtg tctctccctc
     4861 atccaccgga tcgtgctccg gtcaacgttt ccgtttcttg ggccgcgcgc ccgggcgcgg
     4921 ggtcgctcct ggacgcggtg cggcgttgcc cgccgccttt ccatcactcg acttgcccgc
     4981 gctgttggac ccggcctcgc cgtcggtcgc cttgccatcc accaccgcgt ctgacacgtc
     5041 gtcggtgacg ggtgcggcct gcgccttgcg ggcgcggttc ggcttggcgc ccggtggcgg
     5101 cgcgttggcc gcccgtcgct cctgcgcctc gagcttcttc gcctcttcct ccttggcgat
     5161 attcccgaac acgtagtgct gctgtgcgaa cgtccagatg ttgttcgcga accagtacag
     5221 gatgatcgcg atcggcagga acgggccacc gacgacgacg cccaacggga agacgtagag
     5281 cgccagcttg ttcatcatcg cggtctgcgg gttggcggcc gcttcggggc tctgccgcgc
     5341 caccgacgcc cggctgttga agtacgtcgc aatgccggcg gcgatcatca gcggcacacc
     5401 aacagcgatc actgccggac ggctgaattc ggtgaacgcg cccaggccct ggttctgaat
     5461 catggtggcg cccaatgggg cgccgaacag attcgcgtcc aggaagtggc cgacgtccgt
     5521 cgcgctgaat acgtagttgc ccagcgaccg gttcagctcc ggtgacagct gcggctgacc
     5581 gaagccgccc tgcgtccggt tgaacgagcg caacacgtga tacaggccga ggaacaccgg
     5641 gacttgcgca aagatcggca gacagcccat cagcgggttg aacccgtgct cccgctgcag
     5701 cttctgcatc tccagcgcca tccgctgacg gtccttgccg tacttctttt gcagcgcctt
     5761 gatttgcggt tggagttcct gcatctggcg ggtcgtgcgg atctgccgga cgaacggctt
     5821 gtagaggatc gcgcgcagtg tgaagaccag aaacatcacc gacaggcccc acgcgaagaa
     5881 gttcgacggg ccaaggatga aggcgaacgc cttgtaccag acccacatga tcgccgacac
     5941 cgggta
//
LOCUS       DASRJA010000218         4639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1834600,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000218 DASRJA010000000
VERSION     DASRJA010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834600"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VFC01_09725"
     CDS             <1..866
                     /locus_tag="VFC01_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZN79948.1"
                     /translation="AGVGTGGTITGAGRYLKEVSGGKVRIVGVDPEGSVYSGGTGRPY
                     LVEGVGEDFWPSAYDPTVPDEIIAVSDADSFEMTRRLAREEALLVGGSCGMAVVAAVK
                     VAERAGPDALVVVLLPDGGRGYLSKVFNDSWMSSYGFLRTRLDGSVEESTVGDVLRGK
                     SGALPDLVHTHPSETVRDAIGILREYGVSQMPVVGAEPPVMAGEVAGSVSERELLSAV
                     FEGRAKLADAVSVHMSPPLPLIGAGELVSTAAKTLRECDAVMVVEEGKPVGVLTRHDL
                     LGFLSDGNVRR"
     gene            995..1672
                     /locus_tag="VFC01_09730"
     CDS             995..1672
                     /locus_tag="VFC01_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019284015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZN79949.1"
                     /translation="MTDQPPPPPGNYPPPPPPPPSGGYPPPPQGGGYPPPPPAGGGYQ
                     PPPQAGAGFPPAPAGPSPALPQDAYTPWFTRVLAYLIDYIPYAIILGIGWGLLLGTRE
                     TACITDTSEYDLGEFCGTGASTIGQLSIALAGIVAFVYLVWNLGYRQGTTGSSIGKGI
                     MKFKIVSEKTGQPIGFGMSVVRELLYLVAAGICGILWIVAVLFPLWDQKRQTLVDKII
                     STIALPI"
     gene            1696..2373
                     /locus_tag="VFC01_09735"
     CDS             1696..2373
                     /locus_tag="VFC01_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZN79950.1"
                     /translation="MTDQPPPPPGGYQPPPPQGGYPPPPPPSGYPPPPRQGGYPPPPA
                     GAPGYPPAPGAALPKEAYTPWFTRVLGYVIDVVPAAVVIGIGAGIAFGTADNQCTSGG
                     GYDYYCTSSMTAFGMIVYVLAYALVFAYWIWNWGYRQGTTGQSIGKSVMKFKVISEKT
                     GQPIGFGLSIVRQIAHVVDSLVCYIGWLFPLWDAKRQTLADKIMSTICLPAEQSTLNP
                     QPLPPQQ"
     gene            2383..3435
                     /locus_tag="VFC01_09740"
     CDS             2383..3435
                     /locus_tag="VFC01_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79951.1"
                     /translation="MTDQPPRPPGNYPPPPQGGYPPPQPPPGGYPPPPPGGYPPPPTP
                     PGGYAPPPPPPGYAPPPPPPGYGPPPQGGYAPPPQQGGYAPPPQGGYPPPGGYPPSAP
                     QLSVGEAFNWAWGKFRENVAALIVPTLVYGLIVGVLYGIVYGLAFALSPDTVTTYDSS
                     GGGFEYSASSSLGAASFAVLILGGILLLVVVAAIQSAYLAGVLDIANGQPVSIGSFFK
                     PRMVGSVILATVIIGVAGIIGSFCFILGIVISIFTLFTTVAIVDRNLSPIDGIKASID
                     ITKANFVQVLLTWLLAGVIAIVGAIACGIGLIVALPVAALFVVYAYRRLTGGQVAPLN
                     PQPLPPQPPSQFGPPQ"
     gene            3500..4072
                     /locus_tag="VFC01_09745"
     CDS             3500..4072
                     /locus_tag="VFC01_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZN79952.1"
                     /translation="MTEYPPPPPGSYPPPGGYYPPPQRLVLPTEAYTPWVKRLLAFLI
                     DWAPMWLVFLVPMIGLLITDGGDCFDAMYGSSTEYCTTPTSDLWLYIQGLGFLPAVAY
                     FFWNFCYRQGKTGQSIGKSAMKFKVISEKTWQPIGFWLSLVRQIAHYVDQLICYIGYL
                     FPLWDDKRQTIADKLMSTVCVPLDRPPPQQ"
     gene            complement(4073..>4639)
                     /locus_tag="VFC01_09750"
     CDS             complement(4073..>4639)
                     /locus_tag="VFC01_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN79953.1"
                     /translation="RDMTHKLSLRASVTSELHLDDVRLPADAQLPDANGLSGPLSCLS
                     EARFGIVFGSVGAARDCLQTTLDYVGTREVFEKPLAGYQLTQAKIADMAVELGKAQLL
                     ALHLGRLKDDGRIRPEQVSVGKLNNVREAIKIARQCRTLLGANGITLEYPVIRHANNL
                     ESVLTYEGTSEVHQLVIGEALTGVSAFR"
BASE COUNT          761 a         1516 c         1463 g          899 t
ORIGIN      
        1 tcgcgggcgt cggcaccggc ggcaccatca ccggcgccgg gcgctacctc aaagaggtgt
       61 ccggcggaaa ggtgcgcatc gtcggcgtcg accccgaggg atcggtgtac tccggcggca
      121 ccgggcggcc gtatctggtc gagggcgtcg gcgaggactt ctggccgtcg gcgtacgacc
      181 cgacggtgcc ggacgagatc atcgcggtgt ccgacgccga ctcgttcgag atgacgcgcc
      241 ggctggcgcg cgaagaagcg ctattggtcg gcggctcgtg tggcatggca gttgttgccg
      301 cggtcaaggt ggccgagcgg gccgggcccg acgcgttggt ggtcgtcctt ttgcctgacg
      361 gcggaagagg ttatctgtca aaggttttca acgattcgtg gatgtcgtcc tacggcttcc
      421 tgcgcacgcg gctggacggt tccgtcgagg agtcgacggt cggcgacgtg ctgcggggca
      481 agtcaggggc gctgcccgac ctggtccaca cgcatccgtc cgagaccgtg cgcgacgcaa
      541 tcggcatcct gcgcgaatac ggcgtgtcgc agatgccggt tgtcggcgcc gagccgccgg
      601 tgatggccgg cgaagtcgca ggcagcgtgt cggagcgtga acttctctcg gcggtgttcg
      661 agggccgcgc gaagctcgcc gacgccgtct cggtgcacat gagcccgccg ctgccgttga
      721 tcggcgcagg cgaattggtc agcaccgccg ccaagacgct gcgcgaatgt gacgccgtta
      781 tggtggtcga ggagggcaaa ccggtgggcg tgcttacccg tcacgacctc ctcggcttcc
      841 tgtccgacgg caacgttcgt cgatagccgg cccgggtcac cgggaatgtg cgcgaaccgt
      901 gatcaacttc cggttagaaa gccctgctat taggcatctc tcaggttagg gtaagcacgc
      961 tcgttgccag ctaacttcac accggaaggc gtccatgacc gatcaaccgc cacctccccc
     1021 tggtaactac ccgcctccgc ctccgccgcc tccatccggt ggttatccgc ctccgccgca
     1081 gggtggcggt tatccgccgc ctccaccagc cggtgggggt taccaaccgc caccacaagc
     1141 cggcgccggc ttcccgcccg cgcccgccgg tcccagtccg gcgctaccgc aagacgccta
     1201 tacgccgtgg ttcactcgtg tactggccta cctcatcgac tacatcccat acgcgatcat
     1261 cttgggcatc ggctggggtc tgctgctcgg cacgcgcgag accgcctgca tcacagacac
     1321 ttccgagtac gacctcggtg agttctgcgg aaccggtgcg tcgacgattg gtcagctgtc
     1381 gatcgccctt gccgggatcg ttgcctttgt ctacctggtg tggaatttgg gctatcgaca
     1441 ggggacgacg ggctccagca tcggcaaagg catcatgaag ttcaagatcg tcagcgaaaa
     1501 gaccggacag ccaatcggtt tcggcatgtc cgtagtccgg gagctgctgt acctggtggc
     1561 tgcagggatc tgcggcatcc tttggattgt tgccgtcctg ttcccgttgt gggatcaaaa
     1621 gcggcagacg ttggtcgaca agatcatcag cacgatcgcg ttgccgattt agaaacagaa
     1681 gaattggggg atccgatgac cgatcagcca ccgccgcctc ctggcgggta tcaacctccg
     1741 ccgcctcagg gcggatatcc tccgccaccg ccgccgagcg gttatccacc gcctccgcga
     1801 cagggcggtt atccgccgcc cccagcgggg gcgcccgggt atccaccggc gcccggggct
     1861 gcattgccga aagaggctta cacgccgtgg tttacgcgtg tgctcggtta cgtcatcgac
     1921 gtcgtgcccg cggcggtggt catcggtatc ggggcgggca tcgcgttcgg caccgccgat
     1981 aaccagtgca ccagcggcgg tggctacgac tactactgca cctcgagcat gacggccttc
     2041 ggcatgatcg tctacgtcct ggcgtatgcc ctcgtgttcg cttactggat ctggaactgg
     2101 ggctaccggc agggcaccac cgggcagagc atcggcaagt cggtgatgaa gttcaaggtg
     2161 atcagcgaga agaccggcca gccaatcggt ttcggcttgt cgatcgtgcg tcagatcgct
     2221 cacgtcgtcg actcgctcgt ctgctacatc gggtggctgt ttccgctgtg ggacgccaag
     2281 aggcagacgc tggccgataa gatcatgtcg acgatttgtc tgcctgcgga acagtcgacg
     2341 ctcaacccgc agccacttcc acctcagcag taggaagggt caatgaccga tcaaccaccc
     2401 cgtccgccgg ggaactatcc gccaccgccg caaggcggtt acccgccacc gcaaccgcct
     2461 ccgggtggtt accctccacc gccaccgggt ggctatccac ctccgccgac accgccgggt
     2521 ggctatgctc caccgccccc gccgcctggc tatgctccac cgcccccgcc gcctggctac
     2581 ggacccccgc cgcaaggtgg ctacgcacca ccgcctcaac aaggtggcta tgcaccaccg
     2641 ccacaaggcg gttacccccc gcccggtgga tatccgccat cggctccgca gttgagtgtc
     2701 ggtgaggcgt tcaactgggc gtggggcaag ttcagggaga acgtcgcggc gctcatcgtc
     2761 ccgacgttgg tgtacggctt gatcgttggg gtgctgtacg gcatcgtcta cggccttgcg
     2821 ttcgcgcttt ctccggacac cgtcactacg tacgactcgt ccggcggcgg cttcgagtac
     2881 tcggcgagta gttccctcgg cgcggccagc ttcgctgtct tgattctcgg cgggatcctg
     2941 ctgttggtcg tggtggccgc gattcagtcc gcatatctcg caggcgtgct ggacattgcg
     3001 aacgggcagc cggtgtcgat cgggtccttc ttcaagcccc gaatggtcgg cagcgtcatc
     3061 ctcgcgacgg tgatcatcgg cgtcgccggg atcatcggtt cgttctgctt catcttgggc
     3121 atcgtgattt cgatcttcac gttgttcacg accgtcgcga tcgtcgaccg caacctctca
     3181 ccgatcgacg gcatcaaggc gagcatcgac atcaccaagg cgaacttcgt tcaggtgctt
     3241 ctcacgtggc tgctggccgg cgtgatcgca atcgtgggtg caatcgcgtg cggcatcggg
     3301 ctcatcgtcg cgctgccggt cgccgcgcta ttcgtggtgt acgcctaccg tcgcctcacg
     3361 ggcgggcagg tcgcgccgct gaatccgcag ccgctgccac cgcagccacc atcacagttc
     3421 ggcccaccgc agtaacgcgg ctcatcggaa ggcgctgacg ccggtcagcg cttctccggt
     3481 gaagcacaag taccggagta tgacggagta tccaccaccg ccaccaggaa gctatccgcc
     3541 tccgggtggg tactacccgc cgccgcagcg cttggtgctg ccgacggagg cctatacgcc
     3601 gtgggtcaag cgactattgg ccttcctgat cgactgggcc ccaatgtggt tggtgttcct
     3661 tgttccgatg atcggcttgc tcattaccga cggcggcgat tgctttgacg ccatgtacgg
     3721 ttcgagcacc gagtactgca ccacgccgac ttcggacctc tggctgtaca ttcagggcct
     3781 cggcttcctg cctgcggtgg cgtacttctt ctggaacttc tgctaccggc agggcaagac
     3841 gggccaaagc atcggcaagt ctgcgatgaa attcaaggtc atcagcgaaa agacgtggca
     3901 accaatcggt ttctggctgt cgctggtacg tcagatcgcg cactacgtcg atcagctcat
     3961 ctgctacatc ggctatctgt tcccgctgtg ggacgacaag cgacagacca tcgccgacaa
     4021 actgatgagc accgtatgtg tgccgctcga ccggccgccg ccgcaacagt gatcagcgga
     4081 aggcgctgac gccggtcagc gcttctccga tgaccaactg gtgcacctcg gacgtgccct
     4141 cataggtcaa caccgattcc aggttgttgg catgccgaat caccggatac tccagcgtga
     4201 ttccgttggc gcccaacaat gttcggcact gccgggcgat cttgatcgct tcccgcacat
     4261 tgttgagctt gccgacgctg acctgctcag gacggatacg accgtcgtcc ttgagccgtc
     4321 cgaggtgcag cgcaaggagt tgcgccttgc cgagttcgac ggccatatcg gcgatcttgg
     4381 cttgtgtcag ttggtatccc gcgagcggct tctcgaacac ctcgcgggtg ccgacgtagt
     4441 ccagtgtggt ctgcaggcag tcgcgcgcgg cgccgacgct gccgaacacg atgccgaacc
     4501 gcgcctccga taggcagctc agcggaccgg acaaaccgtt tgcgtcgggc agctgggcgt
     4561 cggcgggcag ccgcacgtcg tcgagatgca gttcggaggt gaccgaagcg cgcagcgaca
     4621 gcttgtgcgt catgtctcg
//
LOCUS       DASRJA010000219         3631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2108575,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000219 DASRJA010000000
VERSION     DASRJA010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2108575"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="VFC01_09755"
     CDS             <1..146
                     /locus_tag="VFC01_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HZN79954.1"
                     /translation="VYLLRDSGIHVDIDLQAAIAAAQVAQSVVGHDLPSSLLRAGDRI
                     LGE"
     gene            149..760
                     /locus_tag="VFC01_09760"
     CDS             149..760
                     /locus_tag="VFC01_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN79955.1"
                     /translation="MAVETLSAKGRQTRQAIELAARKLFAERGFHGTTLADITSAAGK
                     SPAAFYRYFADKEDLLAALAESFLHDVMAPSGMSLHLPESPEDDAFFSAVVTGYWNMF
                     KQNIGIMIAVAQLAATQPRFAAVQNEFRRFGIDIVAASVRRAQEQGYGDELDPEHTAA
                     AIALLFENFTTVFVGPSGLGLEISDENAIATLARIWKKTLYGS"
     gene            780..2018
                     /locus_tag="VFC01_09765"
     CDS             780..2018
                     /locus_tag="VFC01_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN79956.1"
                     /translation="MDFALPEHLPGLLAEMDAFIEAEIKPLERENIQYFDQRREHART
                     DWDNGGIPRREWEDLLGEMRKRADKAGWLRYGLPSRFGGRDGTNIDMAVIREHLAHKG
                     LGLHNDLQDESSIVGNFPQVIMMDRFGTETQKAEWTEALITGERSMAFGLTEPSHGSD
                     ATWLETRAESDGDAWVINGAKRFNTGVHRATHDLIFARTSGEPGQARGITAFLVPTDT
                     PGFTVPYYWWTFNMPTDHGEVELKDVRVPEDAVLGEVDRGLEVGQTFLHENRIRQAAS
                     SLGAAQYCIDRAVAYANERTVFGKPLAVNQAVQWPLVELQTEAQMVRLLVYYAATQLD
                     ASHHMEVSDKVSMANYRANRLVCEAADRAMQVFGGVGYSRHEPFEHIYRHHRRYRITE
                     GAEEIQIRRVAQRLFGFGRK"
     gene            2015..2971
                     /locus_tag="VFC01_09770"
     CDS             2015..2971
                     /locus_tag="VFC01_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZN79957.1"
                     /translation="MKEQLAAVLQPVLGDVSIENLRPLTGGASRTTWAFDAVSQQRRA
                     LILRTGPPDDVHAGMELEAAVQQRAKAAGAPVPHILAADNSAAALGNPYLICDAIGGE
                     TIVRRIHRSLDDDGRERLLHQCAQALAAIHRADPDNVGLAHVDQLDEWRQRLDEMGDT
                     TATFEWAFRWLSRHRPPPSPPRLAHGDFRMGNLIVDESGLAAVLDWELVHIGEVYEDL
                     AWFCIRAWRFGAPESLGAGGLGGVESFLSAYEAAAGEHLDRKAFRWWLTLATLRWGVI
                     CRFQAERHLSGQTPSVELAAIGRRVSETEWDVLDLLEGGGPR"
     gene            2968..3321
                     /locus_tag="VFC01_09775"
     CDS             2968..3321
                     /locus_tag="VFC01_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HZN79958.1"
                     /translation="MSWLYGRPTAAELVAAVADFLDNDVRSSTEGQVNFHARVAANVL
                     RMVERELADDSAAEVTGRLAEMGFTDEAQLAATIRAGALDDRADEVLPALRTLVRHRL
                     AVAHPGYDESRPDSS"
     gene            complement(3291..>3631)
                     /locus_tag="VFC01_09780"
     CDS             complement(3291..>3631)
                     /locus_tag="VFC01_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN79959.1"
                     /translation="HGVSMWGYSVLVALDRSAIRTQAALAEAIGADKTRIIAILDELQ
                     DKGLIERVADPEDRRARLLAITKEGRRLKDAVQTEIQRGEERWLGPLSGPDRATFLRV
                     LQELSGLDSS"
BASE COUNT          642 a         1203 c         1203 g          583 t
ORIGIN      
        1 tcgtctacct gttgcgggac agcggaatcc acgtcgacat cgacctgcag gctgcgatcg
       61 ccgcagcaca agtggcgcag agcgtcgtcg gccacgacct gccgagctcg ttactgcgtg
      121 ccggcgaccg catcctcggc gagtaacagt ggctgtcgag acgctcagcg ccaagggtcg
      181 ccagacccgg caggccatcg agctggcggc ccggaagctg ttcgccgagc gtggctttca
      241 tgggaccacg ctagcggaca tcacgtcggc agccggtaag tcgcctgcgg cgttctaccg
      301 ctacttcgcg gacaaggagg atctgctcgc cgcgctggcc gagtcgttct tgcacgacgt
      361 catggcaccg tctggcatga gccttcacct tcccgagtcg ccagaggacg atgcgttctt
      421 ctcggccgtc gtcaccgggt actggaacat gttcaaacag aacatcggca tcatgatcgc
      481 cgtcgcgcag ctggccgcaa ctcagccacg attcgcggcc gtgcagaacg agtttcgccg
      541 cttcggcatc gacatcgtcg cggcgtcggt acggcgggcg caggagcagg gatacggcga
      601 cgagctcgat cccgaacata ccgcggcggc gatcgcgttg ctgttcgaga acttcaccac
      661 cgtcttcgtc ggcccgtccg ggttgggact ggaaatcagc gacgagaacg cgatcgccac
      721 gctcgcgagg atttggaaga agacgctgta cgggtcttag acatcagagg agaatcaccg
      781 tggatttcgc cctcccagag catcttccgg gactgctcgc cgagatggac gcgttcatcg
      841 aggccgagat caaaccgctg gaacgcgaga acatacagta cttcgaccag cgtcgcgagc
      901 acgcccgcac cgattgggac aacggcggca ttccgagacg cgagtgggag gacctgctcg
      961 gcgagatgcg caagcgtgcc gacaaggccg gctggttgcg ctacggcctg ccgtcacggt
     1021 tcggtggccg cgacggcacc aacatcgaca tggcggtgat tcgggaacac ctggcgcaca
     1081 agggtctcgg actgcacaac gatctgcagg atgagtcgtc gatcgtcggc aatttcccgc
     1141 aggtcatcat gatggaccgg ttcggcaccg agacacagaa agccgagtgg accgaggcgc
     1201 tgatcaccgg tgaacggtcg atggcgttcg gcctcaccga accgagccac ggctccgacg
     1261 cgacctggct cgagacgcgc gccgagtccg acggcgacgc ctgggtcatc aacggcgcca
     1321 agcggttcaa caccggcgtg caccgggcca cccacgacct gatcttcgcc cggacctccg
     1381 gagagccggg tcaggcccgc ggcatcactg cctttctagt gcccaccgac actccggggt
     1441 ttacggtgcc gtactactgg tggacgttca acatgccgac cgaccacggc gaggtcgagc
     1501 tcaaggatgt ccgtgtgccg gaggacgcgg ttctcggtga agtcgatcgc ggactcgagg
     1561 tcgggcagac cttcctgcac gagaacagga ttcggcaggc agccagtagc ctgggcgccg
     1621 cgcagtattg catcgaccgc gcggtcgcct acgcgaacga gcgcacggtg ttcggcaaac
     1681 cgctcgcagt caaccaagcg gtgcagtggc cgttggtaga gctgcagacc gaagcccaga
     1741 tggtgcggct actggtgtac tacgcggcga cccaactcga cgccagccac cacatggagg
     1801 tttccgacaa ggtgtcgatg gccaactatc gcgccaaccg gttggtgtgc gaggccgcag
     1861 accgcgccat gcaggtgttc ggcggtgtcg gctacagcag gcatgagccg ttcgaacaca
     1921 tctaccggca ccaccgccgc taccgcatca ccgaaggcgc cgaggagatc cagatccggc
     1981 gggtcgccca gcggctcttc gggttcgggc gcaagtgaag gaacagctcg cagcggtgct
     2041 ccagcccgtc ctcggcgatg tcagcatcga gaatctgcgc ccgctcaccg gtggggcgag
     2101 ccgcaccacg tgggccttcg acgctgtcag ccagcagcgc cgggcgctga tcctgcgcac
     2161 cggtccgccc gacgacgtcc atgccgggat ggagctggag gccgcggtcc agcagcgcgc
     2221 taaggcggcg ggcgctcccg tcccccacat cctggctgcg gacaattccg ctgccgcact
     2281 gggcaatccg tatctcatct gcgacgccat cgggggcgag acgatagtca ggagaattca
     2341 ccgcagcctc gatgacgatg gccgcgagag gctgttgcac cagtgcgcgc aagccttggc
     2401 cgccatccat cgcgccgatc cggacaacgt ggggctggct cacgtcgacc agctcgacga
     2461 gtggcggcaa cggctcgacg agatgggcga caccaccgcc actttcgagt gggcgtttcg
     2521 ctggctcagc cggcaccggc caccgccgtc gccgccacgg ctggcgcacg gcgatttccg
     2581 gatgggcaac ctgatcgtcg acgaatccgg tctggctgcc gtgctcgatt gggaactggt
     2641 gcacatcggc gaggtgtacg aagatctcgc gtggttctgc atccgcgcat ggcgcttcgg
     2701 cgcgcccgag agcctcggag ccggcgggct gggcggcgtc gaaagcttcc tgtcggccta
     2761 tgaagccgcc gcaggggaac acctcgaccg caaggcgttt cggtggtggc tcaccctggc
     2821 cacgctgcgg tggggtgtga tctgccggtt tcaggccgag cggcatctgt cgggtcagac
     2881 gccgtcagtg gaattggccg cgatcggccg gcgcgtcagc gaaaccgaat gggatgtctt
     2941 ggatctgttg gagggaggcg gcccgaggtg agctggctgt acggacgccc gaccgcggcc
     3001 gaactggtcg ctgcggtcgc cgactttctg gacaacgacg tgcgctcgtc aaccgaaggc
     3061 caagtcaact tccacgcccg tgtcgcggcg aacgtgctgc gcatggtcga gcgagaactc
     3121 gccgatgact cggccgcaga ggtcaccggc aggctcgccg aaatgggatt caccgacgaa
     3181 gcacagctgg cggcgacgat ccgtgcggga gcactcgacg accgcgcgga cgaggtgctg
     3241 ccggccctgc gcaccctcgt tcgccatcgg ctggccgtcg cccatccggg ctacgacgaa
     3301 tccaggcctg acagctcctg aagtacccgc aggaacgtcg cgcgatcggg gcccgacagc
     3361 ggtccgagcc aacgctcctc accgcgctga atctccgtct gaacggcgtc cttcagccga
     3421 cggccctcct tggtgatcgc cagcagtcgc gcccgccggt cctcagggtc ggccacccgc
     3481 tcgatcaacc ccttgtcctg gagctcgtcg aggatcgcga tgatgcgtgt tttgtccgcg
     3541 ccgatcgctt cggcgagtgc cgcctgcgtg cggatagccg atcgatcgag ggcgaccagc
     3601 acgctgtacc cccacatcga caccccgtgc g
//
LOCUS       DASRJA010000220         8873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2399329,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000220 DASRJA010000000
VERSION     DASRJA010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8873
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2399329"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFC01_09785"
     CDS             <1..333
                     /locus_tag="VFC01_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN79960.1"
                     /translation="IDQNHAGRLIAEGHASKLLNMRSTLLTLAGGDPMPAAAISKLLS
                     MRTGQHYAEFAVSSFGTDGAIGDPQQLPGSWAQFLLASRATTIYGGTSEVQLNIIAER
                     LLGLPRDP"
     gene            380..1282
                     /locus_tag="VFC01_09790"
     CDS             380..1282
                     /locus_tag="VFC01_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN79961.1"
                     /translation="MRYFAVLGEELNYRRAAERLFITQPALSTAIKQLEHQLGVMLFT
                     RNTREVALTELGQAWLSKVVDALRGIDDAVDDLIALSAHYRRVRLGYLIGTGADLLFR
                     LVRHFEATYPEITLEPREFDFSDPTAGLADGTTEVAIIRPPVDLPEHRMFILDAEDWV
                     ACLPRDHPLAGRTELKITELLDDPIVVAPASAGRWRDYWMAMDARGGKPPTIAAVAAT
                     YEEETTTIARGLGISFTSEASARLYNRQGIAYVPIVDRPRNYTALAWHPAGLSREAAQ
                     LVRHVREEWSFGEGQAVERPATDY"
     gene            1353..2849
                     /locus_tag="VFC01_09795"
     CDS             1353..2849
                     /locus_tag="VFC01_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN79962.1"
                     /translation="MTDTASPVTPAAPANAEDAKLAEFGYTQRLDRSVGRLASFAIGF
                     ATISATTAVFTGFGAGYFTAGAPFVWTLLLAGAVFVVWTFIAADLTAKIPLAGYAYQW
                     TSRIHGSVLAWFTGVMALAGWVCGMTGVGFILSGYLGSLFGWNMSQTAQILVAIAVMA
                     VCVLVNLYGVRFATMVNNIGVSLELVITVGATILVAIVAFSAPENHQPISVLFTGGTS
                     DQHSAYILAWLAASLGPFFGLIGVEASADVAEETVGARHVIPRTMFYALTTSIVIEFL
                     MYVVYVLAIRDQDAVAAASSAPIEEIITQQVGPVVTRIVVAVALTNIMACILANILVA
                     TRLTYSMARDNMLPFSHVWRHVSPSNRTPTYAVLGLFGLSTILLLSALVSEKAFFLII
                     GLSSLAVFATYFLQTVALLIGHRRGTIPAAEPGTFDLGRARMPIYILGIICFTLVCAA
                     LIFLPQFVNNGYVFLGLVVLAGLWAATGLRRRLQSGEAGPDFAKTHLS"
     gene            2873..3886
                     /locus_tag="VFC01_09800"
     CDS             2873..3886
                     /locus_tag="VFC01_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZN79963.1"
                     /translation="MTLELGTSNLVAVEPGAIREDTPPGSVIQYSDYEVDTSSPYAGG
                     VAWIEGEYIPASDARISIFDTGFGHSDLTYTVAHVWHGNIFRLGDHLDRLLDGARKLR
                     LDAGYGKGELAEITKRCVAMSQLRESFVNLTVTRGYGKRKGEKDLSKLTHQVYIYAIP
                     YLWAFPPHEQIFGTTAIVPRHVRRAGRNTVDPTIKNYQWGDLTAASFEAKDRRARTAI
                     LMDADNCVAEGPGFNVVIVKDGTIASPSRNALPGITRKTVFELADAMGIEATLRDVTS
                     HELYEADELMAVTTAGGVTPINTLDGEPIGDGTPGPITVAIRDRFWALMDEPSPLIEA
                     IDY"
     gene            4266..4583
                     /locus_tag="VFC01_09805"
     CDS             4266..4583
                     /locus_tag="VFC01_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79964.1"
                     /translation="MTHDVEKRFDRPGTFHEAAKYVGAVVAAAGVAFAIYATTWRESV
                     VVASLVPAILFAGGVGAFVKTYRVWKEEGAWPAWQGAGWFLLSFMLVCLAIPAAAIHA
                     SMS"
     gene            complement(4740..6443)
                     /gene="fadD2"
                     /locus_tag="VFC01_09810"
     CDS             complement(4740..6443)
                     /gene="fadD2"
                     /locus_tag="VFC01_09810"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD2"
                     /protein_id="HZN79965.1"
                     /translation="MPKLTDLPLQAVAKAQQLAERGSAELHYAYKMLEAGAFKLEPPQ
                     NVAAMLADIRRWGEFGMIPALNARRTPNRTAIIDDFGEITFKELDEAAHEVANGLLEK
                     GVKGGDGVAILARNHRWFLIAVYGAARAGARIILLNSEFSGPQITEVSEREGAKLIIY
                     DDEYFDAVSKADTPLGKLRALGTNPDKEDSASTDETLEDLVARSSGKPAPKVTKHSSI
                     IILTSGTTGTPKGANRSTPPTLAPIGGVLSHVPFKSGEVTSLPAPMFHALGYLHATIA
                     MMLGDTLVLRRRFKPPTVLEDIEKHKVTAMVVVPVMLSRILDALEKMDNKPDLSSLRI
                     VFVSGSQLGAELAQRALKDLGPVVYNLYGSTEIAFATIARPKDLETNPATVGPVVKGV
                     KVKILDDNGNELPQGEVGRIFVGNFFPFEGYTGGGHKQIIDGLMSSGDVGYFDEHGLL
                     YVSGRDDEMIVSGGENVFPAEVEDLISGHPEVVEATALGVEDKEWGHRLRAFVVKTDG
                     ASIGEDDIKTYVREHLARYKVPREVVFLEELPRNPTGKVLKRELREMEVDGADEKTGA
                     E"
     gene            6499..7734
                     /gene="ligD"
                     /locus_tag="VFC01_09815"
     CDS             6499..7734
                     /gene="ligD"
                     /locus_tag="VFC01_09815"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZN79966.1"
                     /translation="MAASMSLDVGGRQVSITHPDKVVFPDIGVTKLDLIHYYLAVSGG
                     ALRGVQDRPMILKRFVKGITEEAVFQKRAPEKRPDFVDVAELKYASGTSAKEAVIHDA
                     AGLVWAVNMGCVDLNPHPVRADDLAHPDELRVDLDPMPGVRWRQIVDVALVAREVLEE
                     HGLTAWPKTSGSRGFHIYARIQRRWPFKFVRLAAQTVAREVERRAPDLATARWWKEER
                     EGVFVDFNQNAFDRTVASAYSVRATPDARVSTPLFWDEVADCQPENFTIATVPKRFED
                     RGDPWEGMDDHAGTLDALLDLADRLGPAEKAPKGAKKGTGRRQSVMPLIEIAQTKTKD
                     EAMAALDEWRARYPSVAEKLEPADILVDGMRGPSSIWYRIRINLQHVPEDQRPPQETL
                     LADYNPWENYSGPQWMRRG"
     gene            7851..8264
                     /locus_tag="VFC01_09820"
     CDS             7851..8264
                     /locus_tag="VFC01_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN79967.1"
                     /translation="MYGNPAYECDGAQVRACSRQLATVVTVTGDLDDANLDKVSQYTK
                     RFVLREKPVVLDLSAVSSATPHIISLLSDIDDACVAAGVEWSLIASQPVNRAVHNFDD
                     RIELPTMASVADALHHFADVTLERRRLLPLLTKSA"
     gene            8400..>8873
                     /locus_tag="VFC01_09825"
     CDS             8400..>8873
                     /locus_tag="VFC01_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HZN79968.1"
                     /translation="MSGSWAAVLGQLIPLALVVAMSPLTIIPAIVLVLQSDRARATGL
                     AFMFGWLVGLAATTAVFVQVPRLLDGLNRPAPAWAAWVRIAVGLALIAFGVWRWLTRH
                     QITKQPAWLGRLSKLTPAGAGAIAVGLILVNPKVLVMNAAAGLVIGTASLGTAGAS"
BASE COUNT         1517 a         2937 c         2789 g         1630 t
ORIGIN      
        1 atcgatcaga atcacgccgg caggctgatc gccgaggggc acgcgtccaa gctgctgaac
       61 atgcggtcga cactgctgac gttggccggc ggcgacccga tgcccgcggc ggcgatctcc
      121 aaactgttgt cgatgcgcac cggccagcac tacgcggagt tcgcggtgtc ctctttcggc
      181 accgacggcg cgatcggcga tccccagcag ctgccgggca gttgggcgca attcctgctg
      241 gccagtcggg ccaccacgat ttacggtggc acatcggagg tgcagctcaa catcatcgcc
      301 gaacggctgc tggggctgcc ccgcgacccg taggcaggca ggagggcggg ccacgagcgg
      361 tgacgtcacg ctgcgccaac tgcgctactt cgccgtgctg ggcgaggagc tgaactatcg
      421 gcgtgccgcg gagcggttgt tcatcacgca gcccgcgctg tcgacggcga tcaaacagct
      481 cgagcatcag ctgggcgtca tgctgttcac ccgcaatacc cgcgaggtgg cgctgaccga
      541 gctgggccag gcctggttgt cgaaggtggt tgacgccctg cgcgggatcg acgatgcggt
      601 cgacgacctg atcgcgctgt ccgcgcacta ccgccgcgtg cggctgggat acctgatcgg
      661 caccggcgcc gacctgctgt tccggttggt gcggcacttc gaagccacct acccggaaat
      721 taccttggag ccaagggaat tcgacttctc tgatccaact gccggcctgg cagacggcac
      781 taccgaggtg gcgatcatcc ggccgccggt cgatctgccc gagcaccgga tgttcatcct
      841 cgacgccgag gactgggtcg cctgcctgcc acgggaccac ccgcttgcgg gtcgcaccga
      901 gttgaaaatc accgagctgc tagacgatcc aattgtcgtc gcccccgcca gcgccggtcg
      961 gtggcgcgac tactggatgg cgatggacgc ccgcggcggc aagccgccca ccatcgccgc
     1021 ggtcgccgcc acctacgaag aggagaccac gaccatcgct cggggcctcg gcatcagttt
     1081 caccagcgag gccagcgccc ggctgtacaa ccggcagggc atcgcgtacg tgccgatcgt
     1141 cgaccggccg cgcaactaca cggcgctcgc ctggcacccg gcgggcctca gccgtgaggc
     1201 agcacagctg gtgcggcacg tccgcgaaga gtggagcttc ggcgaaggac aggcggtcga
     1261 acgccccgcg accgactatt gataaaggcg ctttataagt agatcagaaa ctgcaatttc
     1321 cctggaaacc cgcagtcagg tttcctcact tcatgactga caccgcctca cccgtcacgc
     1381 ctgcggcccc ggccaacgcc gaggacgcca aactcgcgga gttcggctat acccaacgtc
     1441 tcgatcgctc ggtcggccgc ctcgcgtcgt tcgcgatcgg tttcgcgacg atcagtgcca
     1501 ccaccgcggt attcaccggt ttcggcgccg gatacttcac cgcgggcgcc ccgttcgtct
     1561 ggacgctgct actcgcgggc gcggtgttcg tcgtctggac gttcatcgcg gccgacttga
     1621 ccgccaagat cccgctggcc ggctacgcat atcaatggac gagccggatt catggttctg
     1681 tgctcgcgtg gttcaccggt gtgatggcgc tggccggttg ggtctgcggc atgacaggcg
     1741 tcggcttcat cctgtcgggc tatctcggca gcttgttcgg ttggaacatg agccagaccg
     1801 cgcagatcct cgtcgccatc gcggtgatgg ccgtctgcgt gctggtcaac ctctacggtg
     1861 tgcggttcgc gacgatggtc aacaacatcg gagtcagcct cgagttggtc atcacagtcg
     1921 gcgcgaccat cctcgtggcg atcgtcgcgt tctcggcacc cgagaaccac caaccgatct
     1981 ccgtgttgtt caccggcggc acctcggacc agcacagcgc ctacatcttg gcgtggctgg
     2041 cggcgtcgct cgggccgttc ttcgggctca tcggtgtcga ggcaagcgcc gacgtcgcag
     2101 aggagacggt cggtgcccgg cacgtcattc cccgcacgat gttctatgcg cttaccacct
     2161 cgatcgtcat cgagttcctg atgtatgtcg tttacgtgct ggcgatcagg gaccaagacg
     2221 cggtcgcggc cgcgtcgtca gctccgatcg aggaaatcat cacccagcag gtcggcccgg
     2281 tcgtcacgag aatcgttgtg gccgtggcac tgacaaacat catggcctgc atcctggcca
     2341 acatactggt cgccacgcgc ctcacctact cgatggcacg cgacaacatg ctgccgttct
     2401 cccacgtctg gcggcacgtg tcgccgagca accgcacgcc cacctacgcg gtgctcgggc
     2461 tgttcgggct ctcgacgatc ctgctgctgt ccgcgctggt cagcgagaag gcgttcttcc
     2521 tgatcatcgg gttgtcgtcg ttggcggtct tcgcgaccta cttcctgcag accgtcgcac
     2581 tgctcatcgg ccatcgccgg ggaacgattc ccgcggccga acccggaacc ttcgacctcg
     2641 gtcgcgctcg gatgccgatc tacatcctcg gcatcatttg cttcaccctg gtgtgcgcgg
     2701 cgctgatctt ccttccgcag ttcgtcaaca acggctatgt cttcctcgga ctggtggtgc
     2761 tcgccggtct gtgggcggcg accggactgc gccgtcgact gcaatccggt gaggcgggcc
     2821 cggacttcgc caagactcac ctctcctgat tcctgaaagg acttgtcacg caatgactct
     2881 cgaactaggc accagcaacc tcgtcgccgt cgagccgggc gccatccggg aggacacccc
     2941 gcccggatcg gtgatccaat acagcgacta cgaggtggat acgtcgagcc cgtacgcggg
     3001 cggagtggcg tggatcgaag gcgagtacat cccggcgtca gacgcgcgga tctccatctt
     3061 cgacaccgga ttcgggcact ccgatctgac ctacacggtc gcgcatgtgt ggcacggcaa
     3121 catctttcgt ctcggcgacc acctcgatcg gcttctcgac ggagcgcgca agctgcggct
     3181 cgacgccgga tacggcaagg gcgagctggc ggagatcacc aaacgctgcg tggccatgtc
     3241 acagctgcgg gaatcgttcg tcaacctgac ggtcacccgc gggtatggaa aacgtaaggg
     3301 cgagaaggac ttatccaagc tcacccatca ggtctacatc tacgcgatcc cgtacctatg
     3361 ggcgttcccg ccgcacgagc agatcttcgg taccaccgcg atcgtcccgc gccacgttcg
     3421 gcgggctggc cgcaacaccg tcgacccgac gatcaagaac taccagtggg gcgacctgac
     3481 cgcagccagc ttcgaggcca aggaccggcg tgcgcgcacc gcgatcctga tggacgccga
     3541 caactgcgtc gccgagggcc ccgggttcaa cgtggtgatc gtgaaggacg gcacgatcgc
     3601 gtcgccgtca cgaaacgcct tgccgggcat aacccgcaag accgtcttcg aactcgccga
     3661 cgccatgggc atcgaggcga cccttcgcga cgtcaccagc catgagctct atgaggcgga
     3721 cgagttgatg gcagtcacca cggccggtgg cgtcacacca atcaacacgc tcgacggcga
     3781 acccatcggc gatggcacac cgggtccgat cactgtggcg atccgcgatc ggttctgggc
     3841 actgatggat gagccatcac ccctgatcga ggcgatcgat tactgacgtt tgcgcagaaa
     3901 caggtctacc atcgaaatag tcaggtagtc gatcttcgtt agaagtagac atgagcactt
     3961 tcggccgacg cattatgcgt ctcgttgtca ccggtgccgc cgcggtcgct ttgctgaacg
     4021 cgccaaccgt cctattcgct ggcagcgcgc cggaggcttc agctcagccg tgctacagcg
     4081 gcgtcatccc ctggggtccg ttcattccga gctgtacgct cccgggtccg acaggcccca
     4141 gagtccgtgg cgcggcgccc gatgcgaacg caatcatcgc gtgccggaac catcccggtt
     4201 gcctgtcctg gtacgtgaac ggcggtccgt agtttccggg ttcgtgagtt gggaacccca
     4261 aggacatgac gcacgacgtc gagaagcgct ttgaccgtcc cggcacattt cacgaggcgg
     4321 ccaagtatgt cggggcggtt gtcgcggcgg cgggtgtcgc gttcgcgatc tacgcgacga
     4381 catggaggga atccgttgtc gttgcatcgc tggtgcccgc catcctgttc gccggtggcg
     4441 tcggcgcttt cgtcaagacc taccgagtgt ggaaggaaga gggcgcatgg ccggcgtggc
     4501 agggggccgg ctggttcttg ctgtcgttca tgctggtatg cctggccatt ccggcggcgg
     4561 cgatacacgc ctcgatgagc tgaaacacta acggagactt cgcgctaagt cgcacacacg
     4621 cgcacgaatt ttgcagtcag ggctgtcgat ccggaagttt cacgaccctc agtgcaatat
     4681 ccacgccgcc aggggcacga tcgaatccac gcccaggtac taccaccgaa atttaggaac
     4741 tattccgccc ctgtcttttc gtcggctccg tccacttcca tttcccggag ctcgcgcttc
     4801 aacaccttcc ccgtcggatt gcgcggcaac tcctcgagga agaccacctc gcgcggcacc
     4861 ttgtagcggg ccaggtgctc gcgcacatac gtcttgatgt cgtcctcgcc gatgctggcc
     4921 ccgtcggtct tgaccacgaa ggcgcgcaac cggtgtcccc actccttgtc ctccacgccc
     4981 aacgcggtgg cctcgacgac ctcggggtgc ccgctgatca aatcctcaac ctcggcgggg
     5041 aacacgttct cgccgccgga cacgatcatc tcgtcgtcgc gtccgctgac atacagcagc
     5101 ccgtgctcgt cgaagtaacc gacgtcgccc gacgacatca ggccatcgat gatctgcttg
     5161 tggccgccgc cggtgtagcc ctcgaacggg aagaagttgc cgacgaagat ccggccgacc
     5221 tcgccctgcg gcagctcatt gccgttgtcg tcgaggatct tcaccttcac gcccttgacc
     5281 acggggccga cggtggccgg attggtctcc aaatccttgg gccgtgcgat cgtcgcgaac
     5341 gcgatctccg tcgacccata caggttgtaa acgaccgggc ccaggtcctt caaagcacgc
     5401 tgtgcgagtt cggcgcccag ctgcgagccc gatacgaaga caatccgcag cgacgacagg
     5461 tccggcttgt tgtccatctt ctcgagcgca tcgaggatgc gcgacaacat caccggcacc
     5521 acgaccatcg cggtgacctt gtgcttctcg atgtcctcca acaccgtcgg cggcttgaag
     5581 cggcgccgca gcaccagcgt gtccccgagc atcatcgcga tcgtcgcgtg cagatacccg
     5641 agtgcatgga acatcggcgc cgggagcgag gtgacctcac cggacttgaa cggcacgtgc
     5701 gacaacaccc caccgatcgg cgcaagcgtc ggcggcgtac ttctgttggc gcccttggga
     5761 gttccggtcg tgccgctggt caagatgatg atcgatgagt gcttcgtcac cttgggtgcg
     5821 ggcttcccgg agctgcgggc gacgaggtct tccagtgtct cgtcggtgct ggccgagtcc
     5881 tccttgtcgg ggttggtccc cagcgcgcgc agcttgccca gcggtgtatc cgctttagaa
     5941 acggcatcga agtactcgtc gtcgtagatg atcagcttgg cgccctcgcg ctcggaaacc
     6001 tctgtgatct gcggcccaga gaactcgctg ttgagcagga tgatgcgcgc tccggcgcgg
     6061 gccgcgccgt agacggcgat caggaaccac cggtggttgc gagcgaggat tgcgacgccg
     6121 tcgcctccct tgaccccctt ctccagcagt ccattggcca cctcgtgcgc agcctcgtcg
     6181 agctctttga aggtgatctc gccgaagtcg tcgatgatcg cggtgcggtt gggcgtgcgt
     6241 cgcgcgttga gcgcggggat catgccgaat tcgccccacc gtcggatgtc ggcgagcatc
     6301 gcggcgacgt tctgtggcgg ctccagtttg aacgcgccgg cttccaacat cttgtaggcg
     6361 tagtgcagtt ctgccgaccc gcgttcagcg agctgctgag ctttggcgac tgcctgcaac
     6421 ggaagatcgg tcagcttagg catgaaccca ccctatgtga cgcccgtctc accgaggcgg
     6481 ggaaattacc atgacgatat ggccgcttcc atgtcgctgg acgtgggcgg tcggcaggtg
     6541 tccatcaccc accctgacaa ggtcgttttc cccgatatcg gagtcacgaa actcgacctg
     6601 atccactact acctcgctgt ctccggcggt gccctgcgcg gtgtgcagga ccggccgatg
     6661 atcctcaagc ggttcgtcaa gggcatcacc gaagaagctg tcttccagaa gcgcgcaccc
     6721 gagaagcggc cggatttcgt cgacgtcgcc gaactgaagt acgcctcggg cacatcggcc
     6781 aaggaagccg tcatccacga cgccgccggg ctggtgtggg cggtcaacat gggctgcgtc
     6841 gacctcaacc cccaccccgt acgcgccgac gatctcgctc atcccgacga attgcgcgtc
     6901 gacctcgacc cgatgccagg tgtgcggtgg cgccagatcg tcgacgtggc cctggtcgct
     6961 cgggaagtgc tcgaggagca cggcctgacc gcctggccga agacgtcggg atcccgcggt
     7021 tttcacatct atgcccgcat ccagcggcga tggccgttca agttcgtccg gctggcggcg
     7081 caaacggtag cccgcgaggt ggaacgccgt gcgcccgact tggcgacggc gcggtggtgg
     7141 aaagaggagc gcgaaggcgt gttcgtggac ttcaaccaga acgcattcga ccgcacggtg
     7201 gcctcggcgt actccgttcg ggccaccccg gacgcccggg tgtcgacgcc gctgttctgg
     7261 gacgaggtcg ccgactgcca acccgagaac ttcacgattg ccacggtgcc caagcgcttc
     7321 gaggaccgtg gcgatccgtg ggaaggcatg gacgatcacg cgggcactct ggacgcgctg
     7381 ctcgaccttg ccgaccggct ggggcccgcg gaaaaggctc cgaagggcgc caagaaaggc
     7441 accggcaggc gtcagtcggt gatgccgctg atcgagatcg cccagaccaa gaccaaggac
     7501 gaggcgatgg ccgcgctcga cgagtggcgt gcacgttacc cgtcggtagc cgaaaagctc
     7561 gagcccgcag acattctcgt cgacggcatg cggggcccca gttcgatctg gtaccggatc
     7621 cggatcaacc tgcagcacgt gccggaggat cagcggccgc cacaggaaac actgctcgcc
     7681 gactacaacc cctgggagaa ctactcagga ccgcagtgga tgcgccgtgg ttgagcagtt
     7741 tgctgaccgg tccaaaaccg gttcttaata aagtattagc ggttactccc cgttgcctta
     7801 gatcggctcc gtagcttcgt gtacaggtcg gcgaacgagg ggagggtgca atgtacggca
     7861 atccggcata cgaatgcgac ggcgcccagg tccgggcatg cagccgccag ctggccacag
     7921 ttgtcacggt caccggcgat ctcgacgacg cgaacctcga caaggtcagc cagtacacca
     7981 agcgcttcgt cctgcgggag aagccggtag tgctggattt gagcgcagtg agttccgcta
     8041 ctccacacat catttcgcta ctgagcgaca tcgacgacgc ctgcgtcgcc gccggcgtcg
     8101 agtggtcgct tatcgcgagc caaccggtca accgcgcggt ccacaatttc gatgaccgga
     8161 tcgagctgcc gacgatggcg tcggtcgcgg acgcactcca ccacttcgcc gacgtcacac
     8221 tggaacgccg tcggctcctc ccgctcctga ccaagagcgc ctagagacag gaccgatctt
     8281 cagtgcttct cgacattcgt tggctggcct tcctggtaca cggccggcac aagccgcgtt
     8341 cgcggccctt ggcgctcacg gcccgctgat ctcgttctag ctgattaaga tcgctgccca
     8401 tgtcgggtag ttgggcggcg gtgctcggcc agctcattcc gctggcgctc gtggtagcca
     8461 tgtcgccact gacgatcatc ccggcaatcg tgctggtcct gcagagcgat cgggcccgag
     8521 caaccggcct ggccttcatg ttcggctggc tcgtcgggct ggcggcgacg acggccgtgt
     8581 tcgtgcaggt gccccgcctc ctcgacgggc tcaatcgacc ggctccggcc tgggccgcat
     8641 gggtgcggat cgcggtcggc ctcgcgctga tcgcgttcgg cgtgtggcgg tggctgaccc
     8701 gtcaccagat cacgaagcag ccggcgtggc tgggtcggct gagcaagctg acacccgccg
     8761 gcgccggggc tatcgcggta gggctgattc tggtcaatcc caaggtgctc gtgatgaacg
     8821 ccgctgccgg cttggtgatc ggcacggcgt cgttgggcac ggcgggcgct tcc
//
LOCUS       DASRJA010000221        13921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3807464,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000221 DASRJA010000000
VERSION     DASRJA010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13921
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3807464"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VFC01_09830"
     CDS             complement(<1..642)
                     /locus_tag="VFC01_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZN79969.1"
                     /translation="MRIARHVSELIGNTPLVQLNSVVPDGAATVAAKIEYLNPGGSAK
                     DRIAVKMIDAAEASGELKPGGTIVEPTSGNTGVGLALVAQQRGYKCIFVCPDKVSEDK
                     QNVLRAYGADVVVCPTAVPPDHPDSYYSVSNRLVEEIDGAWKPDQYSNPMGPASHYET
                     TGPEVWADTDGKVTHFVAGVGTGGTITGAGRYLKEVSGGKVRVIGADPEGSVYS"
     gene            complement(672..1733)
                     /locus_tag="VFC01_09835"
     CDS             complement(672..1733)
                     /locus_tag="VFC01_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN79970.1"
                     /translation="MTAPSKVSRASHAGVSPARSRRPRKFPVSDGAPVEVLEDGPSIA
                     GRLTALAALLTIRPTLAIGSYAPRLPWPWGLVDFVARVVRPAPGTVRATISLPNCTAQ
                     LVRAGGVLPADGKHSVILYMHGGAFLTCGAHSHGRLVTALSKFADSPVLVVNYRMIPK
                     HSVGQALDDCYDAYKWLRLKGYEPDQIVLAGDSAGGYLALALAEKLQREGLVGEVPAA
                     IVTMSPLFEIDNETRANHPNVRTDVMFPPKAFHALVDLIEEAAERHVVDGKPEEIYEP
                     LDHIEPGLPRTLIHVSGSEVLVSDARKAAHMLAAAGVPVEVRVWPGQMHVFQIATPMV
                     AEANRSLKQIGDYIREATW"
     gene            1931..2896
                     /locus_tag="VFC01_09840"
     CDS             1931..2896
                     /locus_tag="VFC01_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZN79971.1"
                     /translation="MRIRVGRRSTIALAAAAGLASTGSVYVGARNLLSGQADQARQVI
                     PKSWDVPPRADGVYSPGGGPVERWQRGVPFDLHLMIFGDSTAAGYGCESAEELPGVLV
                     ARGLAEESGKRVRLNTKAIVGATSKGLSGQIDAMFVAGPPPDAAVIMIGANDITRPNG
                     IGPSARRLGRAVRRLRASGAVVVVGTCPDFGVITAIPQPLRWYARGRGLRLARAQASE
                     VRSAGGVPVPFSDLLAPEFRKMPEVLFSHDMFHPSGAGYALAAKQLLPALGKALGEFV
                     GHPMVEEALQSRTAEGASLLARVGNISRLWRRSTGVPAPIVVPAS"
     gene            2930..4147
                     /locus_tag="VFC01_09845"
     CDS             2930..4147
                     /locus_tag="VFC01_09845"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZN79972.1"
                     /translation="MTEAVIVSTARSPIGRAGKGSLVDIRPDDLAAQMVRAALDKVPA
                     LDPREIDDLIMGCGQPGGEAGFNIGRAVAVELGYDFLPGTTVNRYCSSSLQTTRMAFH
                     AIKAGEGSAFVSAGVETVSRFPKGTSDGWPDSHNLLFADAEQRSAEAAAGADEWHDPR
                     EDGVLPDVYIAMGQTAENVALLTGISREDQDHWGVRSQNRAEEAIKSGFFDREIVPVK
                     LPDGSTVSKDDGPRAGTTYEAVSQLKPVFRPNGTITAGNACPLNDGAAAVVITSDAKA
                     RELGLTPLARIVATGVSGLSPEIMGLGPIEAVRKALANAGMTIDDIDLYEINEAFAVQ
                     VLGSARELGMDHDKLNVSGGAIALGHPFGMTGARITATLLNNLQTQDKTFGLETMCVG
                     GGQGMAMIVERLS"
     gene            complement(4209..5060)
                     /locus_tag="VFC01_09850"
     CDS             complement(4209..5060)
                     /locus_tag="VFC01_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019734864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79973.1"
                     /translation="MGVGQQPFIGAEALTSGDLTRYELRRYYRAIMPNVYLDKRVEPS
                     LHQRTAAAWLWSGREAVVAGLAASALHGAKWIDNDVPVELIWHNARSPNGVVTRDQLL
                     LENEIQRLDGLAVTSPERTAFDIGRRGRLDDAVARLDALAQATDFKVSAVEELVSRHR
                     HTRGLRQLEAVLDLVDAGAESPKETWLRLLAIRAGYPRPRTQIPVLSPDGRRWYYLDM
                     GWEDLLLALEYDGDHHRTSRERFAYEIERAEDLHVLGWTVVKAAARHRTATVLQRFQR
                     AWDACTH"
     gene            complement(5201..6049)
                     /locus_tag="VFC01_09855"
     CDS             complement(5201..6049)
                     /locus_tag="VFC01_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HZN79974.1"
                     /translation="MRETSNPVFRSLPKTQGGYAQFGTGAAGYGAQAVHDADPYAGAY
                     PEQRQTGVARPLTIDDVVTKTGITLGVLVAVAVAAYFLVAQNLALATPLLFGGLIAGT
                     VLLLVAIFGRKQDNPAIVLSYAACEGLVVGAFSFVVANLMVSGISAGALISQAIVGTF
                     GVFFGMLVVYKTGAIRVTPKFTRFLVAAGFGAVALMLVNLVVGLFTHSAGPLRDGGPM
                     AILFSLLMIGLAAFFFLADFDEADRAIRAGAPEKAAWGIALGLTVTLVWLYLEILRLL
                     SYFNSD"
     gene            6209..7246
                     /locus_tag="VFC01_09860"
     CDS             6209..7246
                     /locus_tag="VFC01_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN79975.1"
                     /translation="MAENEDVLVNVENGIGLITLNRPKAINSLTHPMVTALSKLLTDW
                     EHDDNVRAVVLSGAGERGLCAGGDIVVIYHSAQADGSEARQFWYDEYLLNAQIGRFSK
                     PYVALMDGFVMGGGVGVSAHGSVRVVTDTTKMAMPEVGIGFIPDVGGTFILSHAPGLL
                     GHHAALTGAQFDGADAITLGFADHFVPHDKLPSFKDAIVADGIHAALAAHTQEPPSSQ
                     LLAQRDWIDHCYAGNTVEDIVAALRGHDAGPANDAANLIASRSPISVSVALQAIRRAA
                     KLDTLEDVLRQEYRTSVGALRSHDLVEGIRAQVIDKDRNPKWSPASLAAVTIADVEAY
                     FAPADRELEFT"
     gene            7296..8072
                     /locus_tag="VFC01_09865"
     CDS             7296..8072
                     /locus_tag="VFC01_09865"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZN79976.1"
                     /translation="MTYETILVTRDDRVGTITLNRPKALNALNSQVMNEVVTAAAEFD
                     SDPGIGAIIITGSGEKAFAAGADIKEMADLSFADVFSSDFFAAWGKFAATRTPTIAAV
                     AGYALGGGCELAMMCDLLIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLI
                     LTGRNMDAEEAERAGLVSRVVPADTLLDEANSVAKTIAGMSLSASRMAKEAVNRAFES
                     TLAEGLLYERRLFHSAFATDDQTEGMNAFTEKRPPNFTHR"
     gene            8094..9785
                     /locus_tag="VFC01_09870"
     CDS             8094..9785
                     /locus_tag="VFC01_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HZN79977.1"
                     /translation="MGGTAGTAEAPPREETAKRPWWLRHYTYTGTAIGLVFIWLSLTP
                     SLLPRGPLFQGIVSGAAGAIGYGLGVFGVWLVRYMRSKDTSPPAPRLAWIGLLVAGII
                     GQILMIVWFHMWQDDVRDLNGVPRLGFWDYPLTAVLSIVVLFVLVEIGQLIGKLVRFL
                     VRQLNRFAPPRVSAVVVVALLLALTIALLNGVVVRFAMSTINSTFSAVNDETDPEFPA
                     PTSRLRSGGPESLASWESLGHQGRVFVSAGPTVDELTKFNGKPAIEPIRAYAGLHSAD
                     GIEATAKLAAEELRREGGLDRAIVAVATTTGTGWINEAEASALEYMYNGDTAIVSMQY
                     SFLPSWLSFLVDKENARHAGQALFEAVDAMIREMPEAQRPKLVVFGESLGSFGGEAPF
                     LALNNLVARTDGALFSGPTFNNTIWDELTRNRDPGSPMWLPIYDKGENVRFAARSDNL
                     GRPADPWGHPRVVYLQHASDPIAWWNVDLLFSKPDWLKEPRGYDLSPRTEWIPIVTFL
                     QVSADMAVAVDVPDGHGHVYVRDVANAWADILQPPGWTPEKTEKLRPLLSNDENA"
     gene            complement(9782..10156)
                     /locus_tag="VFC01_09875"
     CDS             complement(9782..10156)
                     /locus_tag="VFC01_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZN79978.1"
                     /translation="MTSRIDGVLDVARARLQRLPASEVPTALSHGAILVDIRPQAQRQ
                     CEGDVRAALVIERNVLEWRCDPTSDSRLPEAVDDDVEWVVLCSEGYTSSLAAASLLDL
                     GLHRATDVVGGYHALKAEGVLV"
     gene            complement(10159..10710)
                     /locus_tag="VFC01_09880"
     CDS             complement(10159..10710)
                     /locus_tag="VFC01_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HZN79979.1"
                     /translation="MVLTHPALLPAAVPAVSAPTRLRLPDLLHATDRYADDVLSGSRD
                     HLLPAEGLPSNDRWFTQLYGDDELDVWLISWVPDRSTELHDHGGSLGALTVVSGALRE
                     TRWDGETLRHRRLAAGDQAAFPLGWVHDVVWTPERDLSAPITGTLSVHAYSPPLTAMS
                     YYEVTERNTLRRKRTELTDVPEG"
     gene            11653..12111
                     /locus_tag="VFC01_09885"
     CDS             11653..12111
                     /locus_tag="VFC01_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79980.1"
                     /translation="MTQSSHANVIHVAAAAGALAAGAVAVAVTGAFDNSSPERAPGAT
                     PVALGAYATPNTLLAAVNEAAVAGQPGATNTGATPTANVAGNTGAPGGTSFFSPAGFF
                     SPTGAPAGVNTSASDPTTPGPVFTPGADPFAVPIHSIRRSGFEWDRGSAT"
     gene            12087..13034
                     /locus_tag="VFC01_09890"
     CDS             12087..13034
                     /locus_tag="VFC01_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79981.1"
                     /translation="MGQGFGDLANAPASAASAANALAENYRETASLIGPEFNATWAKA
                     MEKMAAIEPNGDWGATADQMYRIFVSDGDEPGEDGGLFIGNGADGDPGQDGGKGGLFW
                     GKGGNGGAGVSGVNNGDAGNGGRGGFFYGDGGQGGKGADADVEGNAVAGAGGRGGDSG
                     IYFGVADSGGAGGDATSETGTAQGGAGGAGGNTGLVGTAGSGGAGGTATAPDSATGGD
                     GGDTGWNGNAGTGGNGGNAQTNSGIATAGDGGNGGNNAWDEHGGDAGNGGDATATEGE
                     AHGGDGGDGGAGGWAAPSGRGGDGGDAVIITGTAEGGNG"
     gene            13027..13230
                     /locus_tag="VFC01_09895"
     CDS             13027..13230
                     /locus_tag="VFC01_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79982.1"
                     /translation="MGKGGSSVVGSFFSPGGKGGNGGTGTATNGTATHGFGGLGGLGQ
                     GGAPDGASGSDGGSSDGGSGGTG"
     gene            13248..13679
                     /locus_tag="VFC01_09900"
     CDS             13248..13679
                     /locus_tag="VFC01_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqV"
                     /protein_id="HZN79983.1"
                     /translation="MRRIASGAATVTATSVFVGLVIAGCASGTDREVEPSSPSATTTT
                     TQAPASGGVSVSPDGVTTAVGVPAESTEEEYFHACRAAKVWMDQQGGDPKSLIEPYLK
                     TLQTSDSAGPGTYGTPWSQLAPARQAGVIVAVQAAADALCG"
     gene            13731..>13921
                     /locus_tag="VFC01_09905"
     CDS             13731..>13921
                     /locus_tag="VFC01_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZN79984.1"
                     /translation="MAGKRVALALGSGGARGYAHIGVIEELRDRGYDIVGIAGSSMGA
                     LVGGLQAASKLDEFAEWAKS"
BASE COUNT         2460 a         4501 c         4627 g         2333 t
ORIGIN      
        1 ggagtacacc gatccctcgg ggtcggcgcc gatgacgcgt actttcccgc cggacacctc
       61 cttgagatac cggccggcgc cggtgatggt gccgccggtg ccgacgcccg cgacgaaatg
      121 agtgaccttg ccgtcggtgt cggcccacac ctccggcccg gtggtttcgt agtggctggc
      181 cggccccatc gggttcgagt actggtcggg tttccacgcg ccgtcgatct cttcgaccag
      241 ccggttggag acgctgtagt agctgtccgg atggtcgggc gggacggcgg tcgggcacac
      301 caccacgtcg gccccgtagg cccgcaacac gttttgcttg tcttcgctga ccttgtctgg
      361 gcagacgaag atgcacttgt agccacgctg ctgggccacc agggccagtc cgacgccggt
      421 gttgccggac gtcggctcga ctatggtccc tccgggcttg agttccccgc tggcctcggc
      481 ggcgtcgatc atcttgaccg cgatgcggtc cttggcgctg ccgccggggt tgaggtattc
      541 gatcttggcc gcaactgtgg ctgcgccgtc gggaacaacc gagttcagct gcaccagagg
      601 ggtattgccg atgagctcac tgacgtgccg agcgatgcgc atacgtccat cgtgtcaggc
      661 tcgaggatcg gctaccaggt ggcctctcgg atgtagtcgc cgatctgctt cagcgagcga
      721 ttggcctcag ccaccatcgg ggtggctatc tggaacacgt gcatctggcc cggccagacc
      781 cgcacctcga cgggcacccc cgcggcggcc agcatatgtg cggccttgcg ggcgtcgctg
      841 acgagcactt cggaacccga gacatgaatc agggtgcgcg gaagtcctgg ctcgatgtgg
      901 tcgagcggtt cgtagatctc ctcgggcttt ccgtcgacga cgtgccgctc ggcggcctcc
      961 tcgatcagat cgacgagcgc gtgaaatgcc ttgggcggga acatcacgtc ggtgcggacg
     1021 ttcgggtgat tcgcccgcgt ttcgttgtcg atctcgaaca acggcgacat ggttacgatg
     1081 gcggcaggca cctcgccgac aagaccttcg cgctgcagct tttcggccag ggccaacgcc
     1141 aggtagccac ccgctgagtc cccggccagc acgatctggt cgggttcgta gcccttcagc
     1201 cgcagccact tgtacgcgtc atagcagtcg tccagcgcct gccccacgga gtgcttcggg
     1261 atcattcggt agttaacgac cagaacaggg ctgtcggcga acttcgacaa cgccgtcacc
     1321 agccggccgt gcgagtgcgc accacatgtc aggaatgcgc cgccgtgcat gtagaggatc
     1381 acgctgtgct taccgtcggc gggaagcacg ccaccggcgc ggaccagctg cgcggtgcag
     1441 ttcggcaaag aaatggtcgc gcggacggtt cccggagcag ggcgtaccac gcgcgcgacg
     1501 aagtcgacca aaccccatgg ccagggcagg cgtggcgcat aacttccgat tgctagcgtc
     1561 ggccgaatag ttagcagagc cgccagggct gtgagccgtc ccgcgatgct gggaccgtct
     1621 tccaagacct ctacgggcgc gccgtcactg accggaaact tgcgcggtct acgggatcta
     1681 gctgggctga cgccagcatg cgacgctctg gataccttac tcggtgccgt catcgccacc
     1741 actcctacgc tatcgtagtg ccggggtgcg ccgtgtttac tgggcaaacc caacgaactt
     1801 agcttgacac cctacagcca attcaacaat caacgagtcg aaatcgatac cggacttgat
     1861 accgcactgt gatcgccgag cgggtcttgc cacgcggata agcccggaaa acttccctaa
     1921 acttgttccc gtgcgcattc gtgtggggcg tcggtcgacc atcgccctgg cggcggcggc
     1981 cggactcgcg tcgacgggct ccgtctatgt cggagcccgc aatctgctga gcggacaggc
     2041 tgaccaggcc cgacaggtca tccccaagtc gtgggacgtc ccgccccgcg ccgacggcgt
     2101 ctactcaccc ggcggtggac ccgttgagcg gtggcagcgc ggggtgccgt tcgacctgca
     2161 cttgatgatc ttcggcgact ccaccgccgc cggctacggc tgcgagtccg cggaggagct
     2221 gcccggtgtg ctcgtcgccc gagggctggc cgaagaatcg ggcaagcgcg tgcggttgaa
     2281 cacgaaggcg atcgtcggcg cgacgtccaa ggggctgtcg ggccagatcg acgcgatgtt
     2341 cgtggcgggc ccgccgccgg acgccgccgt gatcatgatc ggcgcgaacg acatcaccag
     2401 gccgaatggc atcggaccgt cggcacgccg actcggccgg gcggtgcgcc ggttgcgcgc
     2461 cagcggagcc gtcgttgtgg tcggcacctg tccggacttc ggtgtgatca ccgccattcc
     2521 gcagccgctg cggtggtacg cgcgcggccg cgggttgcgc ctggcccgtg cgcaggcctc
     2581 agaagtccgg tctgcgggtg gcgtccccgt tccgttctcc gacttgttgg cgcccgaatt
     2641 ccgcaagatg ccggaagtgc tgttcagcca cgacatgttc cacccgtccg gcgccggata
     2701 tgccttggcc gccaagcagt tgctgcccgc gctcggtaaa gcgttgggag agttcgtcgg
     2761 tcacccgatg gtcgaagaag cgttgcaatc gcgaacggcc gaaggcgcat cgttgctggc
     2821 tcgggtcggc aacatcagca ggctgtggcg tcgttcgact ggggtgcccg cgcccatcgt
     2881 ggtgcccgcc agctaggttg ctcgtatcca ctcgatacga ggagccgtca tgactgaagc
     2941 cgtcatcgtt tcgaccgcgc gttcgccgat cggccgcgcc ggtaagggtt cgctggtcga
     3001 tattcggccc gacgacctcg ccgcccagat ggtgcgcgcg gcgctggaca aggtgcctgc
     3061 tttggatccg cgcgagatcg acgatctgat catgggctgc gggcagcccg gcggtgaggc
     3121 cggcttcaac atcggtcgcg cggtggccgt cgagctgggc tacgacttct tgccaggcac
     3181 cacggtgaac cgatactgct cgtcctcgct gcagaccacg cggatggcgt tccacgcgat
     3241 caaggccggc gaaggcagcg cgttcgtttc cgctggggtg gaaacggttt cgcgtttccc
     3301 gaagggcacg tcggatggct ggccggactc gcacaacctg ctgttcgccg atgcggagca
     3361 gcgcagcgcg gaggccgccg cgggcgccga cgagtggcac gaccctcgtg aggacggcgt
     3421 gctgccggat gtctacatcg cgatgggcca gaccgcggag aacgtcgcgc tgctcaccgg
     3481 catcagccgc gaggaccagg accactgggg cgtgcgttcg cagaaccggg ccgaggaagc
     3541 catcaagagc ggcttcttcg atcgcgagat cgttccggtg aagctgcccg acggctcgac
     3601 ggtgtccaag gacgacgggc cgcgggccgg caccacctac gaggcggtct cgcaattgaa
     3661 gccggtgttc cggccgaacg gcaccatcac cgcgggcaat gcgtgtccgc tgaacgacgg
     3721 ggctgccgcg gtagtgatca cgtcggacgc caaggcgcgc gagctggggc tgacgccgtt
     3781 ggcgcggatc gtcgcgacgg gcgtgtccgg gttgtcgccg gagatcatgg gcctaggccc
     3841 gatcgaggcc gtccgcaagg cgctggccaa cgcgggcatg acgatcgacg acatcgacct
     3901 gtacgagatc aatgaggcgt tcgcggtcca ggtgctgggt tcggcccgcg agcttggcat
     3961 ggatcacgac aagctcaacg tgtccggcgg cgcgatcgcg ttgggtcacc cattcggcat
     4021 gacgggcgcg cggatcaccg ccacgctgct gaataacctt cagacacagg acaagacgtt
     4081 cggtctggag acgatgtgcg tcggcggcgg ccagggcatg gcaatgatcg tggagcgact
     4141 cagctgaccg acgttgactc tgcgctcatg gcgcaaaagt gcgagtagcc aacgccgtca
     4201 gcgcagagtc aatgagtgca cgcatcccaa gcacgctgga agcgctgcaa gacggttgcg
     4261 gtccgatgcc gcgcagccgc tttgacgacg gtccagccca acacgtgaag gtcctccgcg
     4321 cgttcgattt cgtatgcgaa tcgctcgcgg ctggtccgat ggtgatcgcc gtcgtattcc
     4381 agggccagca ataggtcctc ccaccccatg tcgaggtaat accatcggcg gccgtcgggg
     4441 cttaacaccg gaatctgcgt ccgtggtcgc gggtagccag cgcgaattgc caagagccgc
     4501 aaccacgttt ccttcggcga ctcggcgccg gcatcaacta ggtccagcac tgcttcgagc
     4561 tggcgtagac cacgcgtgtg tcgatggcga gagaccaatt cttcgaccgc cgacaccttg
     4621 aagtcagtgg cctgcgccaa cgcgtccaac cgagcgacgg catcatcgag ccgaccccga
     4681 cgtccaatgt cgaaggctgt gcgctcaggg ctcgtcaccg cgagaccgtc gagtcgttga
     4741 atctcgttct caagcaagag ctgatctcgc gtcaccacgc cgttcggtga ccgcgcgttg
     4801 tgccagatca attcgactgg cacgtcgttg tcaatccatt tcgcaccgtg gagtgccgac
     4861 gctgcaagcc ccgccactac tgcctcgcgg ccggaccata gccaggccgc ggcggtgcgc
     4921 tgatgcaagg acggttctac ccgcttgtct aggtagacat tcggcatgat cgctcggtag
     4981 tacctgcgca attcgtagcg tgtcaggtca ccagaagtca gtgcttcggc acctatgaat
     5041 ggctgctgtc ccacccccat gccggcagat tgccagcgat cggagacacg aatcgttgac
     5101 cctgcgtcca cggcgcaaaa ttgtggataa cccccgccct gtgcgcagag tcaacgaaaa
     5161 aggcccggta catttcgtgc cgggccttct cgtcaggctg ctagtcgctg ttgaagtagc
     5221 tgagcagccg caggatctcc aggtacagcc agaccagcgt cacggtcagg ccgagcgcga
     5281 tgccccatgc ggccttctcc ggcgcgccgg cgcggatcgc ccggtcggcc tcatcgaagt
     5341 cggccaggaa gaagaacgcc gccaggccga tcatcaagag tgagaagagg atcgccatcg
     5401 ggccaccgtc gcgcagcggt ccggcactgt gagtgaacag ccccactacc aagttgacga
     5461 gcatcagcgc gaccgcgccg aagccggcgg caacgaggaa gcgggtgaac ttcggcgtca
     5521 cgcgaatggc gccggtcttg tacacgacca gcatgccgaa gaacacgccg aaggtaccca
     5581 cgatcgcctg gctgatcagc gcacccgcgc tgatgccgga caccatcagg ttggcgacga
     5641 caaacgagaa cgcgccaacg acgaggccct cacatgcggc gtagctcagc acgatggccg
     5701 gattgtcctg cttgcgaccg aagatcgcga caaggaggag caccgttccc gcgatcagcc
     5761 cgccaaacag aagcggggtg gccagcgcga ggttctgcgc gaccaagaag tacgcggcga
     5821 ccgcgacggc caccagcacg ccgagcgtga tgcccgtctt ggtgacgacg tcgtcgatgg
     5881 tcagcggccg ggcgactccg gtctgccgct gttcggggta cgcgccggcg tatgggtcgg
     5941 catcgtgtac cgcctgcgca ccgtagccgg cggctccggt accgaattgc gcgtaaccgc
     6001 cctgcgtctt cggcagggat cgaaataccg ggttgctggt ctcccgcacc gtcggttcct
     6061 ctcctagtgc tgtggttcga caaatttgcg aacacactct caacgatcag ctacctggtg
     6121 ttggttcccg gagaaacctc ccagctagtt cacagaaaac cctacccggc gctgctcttc
     6181 tccgggccac gatctacatt gcataaccgt ggcagaaaac gaggatgtcc tagtaaatgt
     6241 cgagaacggc atcggtctga tcaccctcaa ccgccccaag gcgatcaact cactgaccca
     6301 tccgatggtc accgcgctgt cgaagttgct caccgactgg gagcacgacg acaacgtccg
     6361 cgcggtggtg ttgtccggcg ccggtgagcg cggcctctgc gcaggcggcg acatcgtggt
     6421 gatctatcac agcgcgcagg ccgacgggtc cgaggcgcga cagttctggt acgacgaata
     6481 tctgctcaac gctcagatcg gtcgcttcag caagccgtat gtcgcactca tggacggctt
     6541 tgtcatgggc ggcggagtcg gggtgagcgc gcatggcagc gtccgcgtcg tcaccgacac
     6601 cacaaaaatg gcgatgcccg aagtgggcat cggcttcatt cccgacgtcg gtggcacctt
     6661 tatcttgtcg cacgcgcccg gcctgctcgg tcatcatgcc gcgctgacgg gcgcccagtt
     6721 cgacggcgcc gacgcgatca cgctgggctt cgccgaccat ttcgtgcccc acgacaagct
     6781 gccttcattc aaggatgcga tcgtcgccga cggcatccac gcagcgctgg ccgcgcacac
     6841 gcaagagccg ccgtcgagtc agctactggc acagcgggat tggatcgacc actgctacgc
     6901 cggcaacacc gtcgaggaca tcgtcgcggc actgcgcggc cacgacgcgg gtcccgctaa
     6961 cgacgccgcc aacctgatcg ccagccggtc ccccatctcg gtgtccgtgg cgctgcaggc
     7021 catccgccgc gccgccaagc tcgacacgct ggaagacgtg ctgcgccagg aatatcggac
     7081 ctctgtcggt gcgctgcgct cgcatgatct agtggagggc atccgcgccc aagtcatcga
     7141 caaagaccgc aatccgaaat ggtcgcccgc gtcgctggcg gccgtcacca tcgccgacgt
     7201 cgaggcctac ttcgcgccgg ccgaccgcga actggagttc acatgagcac cgaaggcgac
     7261 aacgagcgag gaccggagcg aggaggagcc gagaaatgac ctacgagacc atcctcgtca
     7321 cccgtgatga tcgcgtcggc accatcacgt tgaatcggcc gaaggcactg aacgccctca
     7381 acagccaggt gatgaacgaa gtcgtcacgg cggcagcgga attcgacagc gacccaggca
     7441 tcggcgcgat catcatcacc ggatccggcg agaaggcgtt cgccgcgggc gcggatatca
     7501 aggagatggc cgacctgtcg ttcgccgacg tgttctcctc cgacttcttc gcggcgtggg
     7561 gtaagttcgc cgccacccgc accccgacaa tcgccgcggt cgcgggctat gcgctcggcg
     7621 gcggctgcga gttggcgatg atgtgcgatc tgctgatagc cgccgacacc gcgaaattcg
     7681 gccagcccga gatcaagctc ggcgtgctgc ccggcatggg cggctcacaa cggctcaccc
     7741 gcgcgatcgg caaggcgaag gcgatggacc tgatcctgac cggccgcaac atggacgccg
     7801 aggaggccga gcgcgccggg ctggtgtcgc gcgtggtgcc cgccgacacg ctgctcgacg
     7861 aagccaactc cgtcgccaag accatcgcgg gcatgtcgct gtccgcgtcg cggatggcca
     7921 aggaggccgt gaaccgcgcg ttcgaaagca cgctggccga agggctcctc tacgagcgcc
     7981 gtctgttcca ctccgcgttt gccaccgacg accagaccga aggcatgaac gcgttcactg
     8041 agaaacgccc cccgaacttc acgcaccgct aatcttcgtt cgtgacaaat accctgggcg
     8101 gcaccgcggg aaccgcggag gcaccgccgc gggaggaaac cgccaagcgg ccgtggtggc
     8161 ttcgccacta cacctacacc ggtaccgcga ttggtctggt gttcatctgg ctctcgctca
     8221 cgccgtcact gttgccgcgc ggaccgcttt tccagggcat cgtcagcggc gcagccggcg
     8281 ccatcggcta cggcctcggc gtgttcggag tatggctggt gcgctacatg cgctcgaagg
     8341 acaccagccc cccggcgccg cggctggcgt ggattgggct gttggttgcc ggaatcatcg
     8401 gccagatcct gatgatcgtc tggttccata tgtggcagga cgacgtgcgc gacctcaacg
     8461 gtgtgccgcg actggggttc tgggactacc cactgaccgc cgtcctgtcg atcgtcgtgc
     8521 tgtttgttct cgtcgaaatc ggccagctga tcgggaaatt ggtgcgcttt ctggtgcgtc
     8581 aactcaatcg ctttgcaccg ccgcgtgttt cggcagtcgt tgtggttgcg ttgttgcttg
     8641 ccttgaccat cgcgctgctc aacggcgttg tggtgcggtt cgcgatgagc acaatcaata
     8701 gcacgttctc cgccgttaac gacgagaccg atcccgagtt tccggcaccg acgtcgcggc
     8761 ttcggtccgg cggccctgag tcgctggcca gttgggagtc actcggtcac cagggccggg
     8821 tgttcgtgtc cgcggggccg acggtcgacg agctcaccaa gttcaacggt aagccggcga
     8881 tcgaaccgat ccgcgcttat gccggactgc attccgccga cggcattgag gcgaccgcga
     8941 agctcgccgc ggaggaattg cgacgagaag gcggactgga ccgcgcgatc gtcgccgtcg
     9001 ccaccaccac cggcaccggc tggatcaacg aggccgaggc gtcggcgctg gagtacatgt
     9061 acaacggcga caccgcgatc gtgtccatgc agtactcgtt cctgcccagc tggctgtctt
     9121 tcctggtgga caaggagaac gcgcgccatg ccgggcaggc gctgttcgag gccgtcgacg
     9181 cgatgatccg ggagatgccc gaagcgcaac gacccaagct cgtcgtgttc ggcgaaagcc
     9241 tgggctcatt cggcggtgag gcgccgttcc tggcgctcaa caacctcgtg gcccgcaccg
     9301 acggcgcgct gttctccggg ccgacgttca acaacacgat ctgggacgag ctcacccgca
     9361 accgcgaccc gggctccccg atgtggctgc ccatctatga caagggtgag aacgtccgct
     9421 tcgccgcacg ctccgacaat ctcggccggc ccgccgatcc gtggggccat ccgcgggtgg
     9481 tgtatctaca gcacgcgtcc gatccgatcg cgtggtggaa cgtcgatctg ctgttctcca
     9541 aaccggattg gctgaaagag ccccgcggct acgacctctc gccgcggacg gaatggatcc
     9601 cgatcgtgac gttcctacag gtgtccgccg atatggcggt cgcggtagac gtgcccgatg
     9661 gccacggcca cgtctacgtc agggacgtcg ccaacgcatg ggctgacatc ctgcagccac
     9721 cgggctggac gccggagaag acggagaagc tgcggccgct gctgagcaac gacgaaaacg
     9781 cttagaccag aacgccttcg gccttcaagg cgtgatagcc gccgacgacg tcggtggcgc
     9841 ggtgcaggcc gagatcgagc agcgacgcgg ccgccaggct cgaggtgtag ccctctgagc
     9901 acagcaccac ccattccacg tcgtcgtcga cggcctccgg cagtcgcgag tcgctggtcg
     9961 gatcgcagcg ccactcaagc acattccgct cgatcaccag tgcggcacgc acgtcgccct
    10021 cacactggcg ctgggcctgc gggcggatgt cgaccaggat cgcaccatgg ctcagagcgg
    10081 tgggcacttc cgatgcgggg agccgttgta gacgggctct ggcaacgtcg agaacaccgt
    10141 cgatgcggct ggtcatcgtc atccctccgg aacgtcagtg agctcagtgc gcttgcggcg
    10201 caacgtattt cgttcggtga cttcgtagta cgacatcgcg gtcaggggtg gcgagtacgc
    10261 gtgcacactc aacgttccag tgatgggagc cgacaggtcc cgttccggtg tccacacgac
    10321 gtcgtgcacc cagcccagag ggaaggcggc ctgatcacca gccgcaagtc ggcgatgccg
    10381 tagcgtctcg ccgtcccagc gtgtttcacg cagcgcgccc gacaccacgg tcagcgctcc
    10441 cagggagccg ccgtggtcgt ggagttccgt cgagcgatcg ggcacccagc tgatcagcca
    10501 tacgtcgagt tcgtcgtcgc cgtacaactg cgtgaaccac cgatcgtttg agggcagacc
    10561 ctcagccggc agcaggtggt cgcgggagcc ggagagcacg tcgtcggcgt agcggtcggt
    10621 ggcgtgcaac agatcgggca gacgcagtcg agtcggcgcg gaaacggcag ggacggcagc
    10681 gggcaacaac gcagggtggg tgagaaccat gcagggctcc aaggaagacg acgaacggga
    10741 tcgggggtcg gcgttaggcc ggacaacact ccagagatcc gaacttgttc gtcacgacgg
    10801 caagtgttgc acatatcgcc gggcgtcgca tcgcgcaacc ctgccgccga cccccggcgc
    10861 gagcccggcc gaccacgccg tcgcgtgccc tgccgctcgc gtcagtttga gactgctcaa
    10921 gtatcccggc catatgcgga actggattgc tgtgggtaat cgactgactt tgcgcgctgc
    10981 agtccgtgga agacgtctta acacaaacac acccgagcgg atagagaatc atgatcttct
    11041 catgcgattg tcctgggcca tgcccatgcc ggacaaataa atttgcggtc gcgttattta
    11101 atcgactatc gcgggtgtga gcatttcctt atgccgttcg catcgcaaac gatctcggcc
    11161 gcctcaaatg ccgccgcgag gcgctaatgc cgtcggtatg cgagccctaa caaggtcatt
    11221 cctgttcgga atctcgtaca tgtgaaccgg aaatgcagcg gcgcaaccct tcctgcgcgg
    11281 ggcagccgct cgttcgaggg cctgtgagta ggcacttcgc accgattgaa tgcaatcgat
    11341 gccacgggat cgtgcaaagg taaacacatc tagcaaaagg tgcgtccacc tagccacgat
    11401 tacacccagc gtgcgcggcc atccgaatga gattcgccac cggccgttcg aagcgctcga
    11461 cgatcacgtg tttctcgggt cacatgctag ctggttcggt gagcttgaga agaagctggc
    11521 gacaattatt cgaatcaatt gctatgttgc tgacgatggg tgtaaagata tttgctgtca
    11581 ggttcctatt tacgtctgta gccgcggagt catacacccg gggggatgga ccgtgatatg
    11641 agggggaagc acgtgacaca gagttcgcac gcgaacgtga tccatgtcgc agcggccgcc
    11701 ggtgctctgg ccgctggcgc ggttgctgtc gcggtgaccg gtgctttcga caacagttcg
    11761 ccggaacgtg caccgggagc cactccggta gccctgggcg cctacgcaac gccgaacacc
    11821 cttctcgcag ccgtgaatga ggcggccgta gcgggccagc cgggggccac caacacgggc
    11881 gcgaccccaa cggcgaacgt tgcgggtaat accggtgcgc ctggcggcac aagcttcttc
    11941 agccccgcgg gcttcttcag cccgacgggc gcgcccgccg gcgtcaacac ctccgccagc
    12001 gacccgacca cccccggccc ggtgttcacc cccggtgccg atcctttcgc ggtgccaatc
    12061 cattcgatcc ggcgttctgg attcgaatgg gacaggggtt cggcgaccta gccaacgcgc
    12121 cggcgtcagc agcgtctgcc gccaacgctt tggctgagaa ctaccgagaa accgccagct
    12181 tgatcggccc tgaattcaac gccacgtggg caaaggcgat ggagaagatg gctgcgatcg
    12241 agccgaacgg tgactggggg gccaccgccg accagatgta tcgcatcttc gtcagcgacg
    12301 gcgacgagcc cggcgaagat ggcggcctgt tcatcggcaa cggcgccgac ggcgacccgg
    12361 gacaggacgg cggcaagggc ggtctgttct ggggtaaggg tggcaacggc ggtgccggtg
    12421 tgagtggagt gaacaacggc gacgctggca acggcggccg cggcggcttc ttctatggcg
    12481 acggcggcca gggcggtaaa ggcgcggacg cggacgtcga aggcaacgcg gttgcggggg
    12541 ccggtggtcg cggcggggac agcgggatct atttcggtgt cgccgatagt ggcggtgccg
    12601 gcggggacgc caccagcgag accggcacgg ctcaaggcgg tgccggcgga gccggcggca
    12661 acaccgggct tgtcggtacc gcaggtagtg gcggcgcagg cggcacggcg acggccccag
    12721 actcggcgac gggcggcgac ggcggtgaca ccggctggaa cggtaacgcg ggaaccggcg
    12781 gtaacggcgg caacgcgcaa accaactcgg gcatagccac ggccggggac ggtggcaacg
    12841 gcggcaacaa cgcctgggac gagcacggcg gggacgccgg caacggcggc gatgccacgg
    12901 ccaccgaggg tgaagctcac ggcggtgacg gcggtgacgg cggcgcaggc ggctgggccg
    12961 caccgagcgg acgcggcggt gacggcggcg atgcggtcat catcaccggt acggctgagg
    13021 gcggtaatgg gtaaaggcgg tagctcggtg gtcggcagct tcttctcccc tggtggcaaa
    13081 ggcggcaacg gcggcaccgg aaccgcgacc aacggcacgg caacccacgg cttcggcggc
    13141 ctgggaggct tgggccaggg cggtgctccg gatggagcct cgggcagcga cggcggctcc
    13201 tccgatggtg gttcgggcgg caccgggtag ccgcatagat tggcgggatg cgaaggatcg
    13261 cttctggcgc cgcaacggtc acggcgacat ccgtcttcgt cggcctggtg atcgccggct
    13321 gcgcttcggg cacggatcgc gaggtggaac cgtcgtcgcc gtctgcgacc acgacgacca
    13381 cccaagctcc ggcgtccggc ggggtgagtg tctcgcccga cggcgtcacg acggccgtcg
    13441 gcgttccggc ggaatccacc gaggaggagt acttccacgc gtgtcgcgcc gcgaaggtgt
    13501 ggatggacca acagggtggt gaccccaaga gcctgatcga gccctacctc aagacattgc
    13561 agacgtcaga ctccgccggc cccgggacct acggcacccc gtggtcgcag ctggcaccag
    13621 cgcgacaggc gggcgtgatc gtcgccgtgc aggcggccgc cgacgcactg tgcggctaga
    13681 tcgccgaatt cgaatcacgc tgagtttatt cggcgcgcca caatagcgat atggccggca
    13741 aacgcgtcgc gctggccctc ggcagcgggg gcgcccgcgg ctacgcccac atcggcgtca
    13801 tcgaagaact acgcgatcgc gggtacgaca tcgtcggcat cgccgggtcg tcgatgggtg
    13861 cactcgtagg cggtctgcag gccgcgagca agctcgacga attcgcggag tgggcgaagt
    13921 c
//
LOCUS       DASRJA010000222         7093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4091353,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000222 DASRJA010000000
VERSION     DASRJA010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4091353"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1432)
                     /locus_tag="VFC01_09910"
     CDS             complement(176..1432)
                     /locus_tag="VFC01_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-acting enoyl reductase family protein"
                     /protein_id="HZN79985.1"
                     /translation="MSAAQREFDIVLYGATGFVGKLTAEYLARAGGDARIALAGRSQD
                     KLRAVRDTLGEKAQSWPLLAADASQPSTLDAMAAQTQVVVTAVGPYLKYGLPLVAACA
                     AAGTDYADLTGEALFIRESIDLYHKQAIDTGARIVHSCGFDSIPSDLTVFALYRRAIE
                     DDAGQLTDTNMVVRAFAGGVSGGTVASMIELLRTASSDPEARRLMNDPYTLTPDRGAE
                     PELGAQRDVRWRRGREIAPELNGYWMGAFVMAAANTRIVRRSNALLDYAYGKRFEYAE
                     QMSLGKSILAPLAAAMATGGNAATMALGGRYFNRLPNKLVERIAPKPGTGPSERAREQ
                     GRYAVETYTTTTTGARYRSTMSQKGDPGYKATSVLLGESGLALALDRDRLSELRGVLT
                     PAAAMGDALMARFPAAGVSLETVRLN"
     gene            1473..1817
                     /locus_tag="VFC01_09915"
     CDS             1473..1817
                     /locus_tag="VFC01_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN79986.1"
                     /translation="MLDGAMIVWFALTAASVAFVVADSVFNGVTSWVQRTAWILVTAY
                     TGPVGVFLYLLACRRPLPGTHDAFTSATWKQSLNSEMHCLAGDATGIVVRPRSCRCSA
                     SPTAGTPPSRSC"
     gene            1769..2176
                     /locus_tag="VFC01_09920"
     CDS             1769..2176
                     /locus_tag="VFC01_09920"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HZN79987.1"
                     /translation="MFGLTNGWDATVAIMLILGAHWPGSEDPHHPSFWFRMSVATIVG
                     GVVAYPINHWLVAKRLKHGCMTLPGSDAPAPGLGHVSPEPSMMMDHAATAQQSEHTMT
                     ELPAARAVAIIAGTFAILLVAVWLTNLVVPVRF"
     gene            complement(2181..4154)
                     /locus_tag="VFC01_09925"
     CDS             complement(2181..4154)
                     /locus_tag="VFC01_09925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZN79988.1"
                     /translation="MATTPFDDLDEFLALPRVSGLAVSPDGSRVVTTIAELNDKRTEF
                     VTAIWELDPAGRRPARRLTRGAKGESSPAFTSDGDLLFVAARPTEEDDKPPASLWRLP
                     AAGGEAVVALALPGGVEDVRAARTMAVTVVAAKLLRSASGMDEDRRLRSLRKDNTVSA
                     VLHSGYPVRYWDHDLGPDHPHLLDIDGPRDLTPQPGDALREADFDMSSDGSFVVTSWH
                     GPGPGASIRGTLVRIDHATGQRTTIADDPDADLESPAISPDGTAVAFTRETHSTPDKA
                     PRITLCHMHFGEPFVELTGDWDRWPTSVTWSRDGSSLIVTADEAGRGPIFAIDPASGA
                     VTRLTDDDYTYSDVRTAPDGVVYAMRSSYARPPHPVRIDPDGTVTELPSVAAPALPGT
                     LTEVAAIADDGQPVRSWLALPHGDAPAPLVLWVHGGPLASWNAWHWRWNPWLLVAEGY
                     AVLMPDPALSTGYGQDFIQRGWGAWGFAPYTDLIAATDAACAHPRVDETRTAVMGGSF
                     GGYMANWIAGHTDRFDAIVTHASLWALDQFGSTTDGAYWWAREMTPEMAEQNSPRRFV
                     AQIRTPMLVIHGDKDYRVPIGEALRLWFELLTESGLPASDDGTSPHRFLYFPSENHWV
                     LTPQHAKLWYQVVLGFLARHVLGEDVELPEALG"
     gene            4232..4621
                     /locus_tag="VFC01_09930"
     CDS             4232..4621
                     /locus_tag="VFC01_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79989.1"
                     /translation="MSTVVLGELAGRACPIAAALELVGERWALLVVREIALGATHFSD
                     IVAGTGAPRDRIAARLKALESAGVVVRSPYQSAPTRYEYRLTTSGEALIPVLDALLAW
                     GKVHAVSPDDPDRQQKYRPFSELRKSR"
     gene            4618..5148
                     /locus_tag="VFC01_09935"
     CDS             4618..5148
                     /locus_tag="VFC01_09935"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN79990.1"
                     /translation="MTAELVDTDIQADWGPQRSRTVTWYSPGPSTAKGLSMSGIEYLR
                     AMVDGDLPQPPIAGLMQFDLVEVEPGRAVFTCVPDESAYNPIGAIHGGLICTLLDSVT
                     GCAVHTTLSQGKGYTSVEIKVNYLKAVQLTSGLLTATGTVVKTGSRVGFAEGVITDAS
                     GAVVATATSTLLIFDL"
     gene            5177..6346
                     /locus_tag="VFC01_09940"
     CDS             5177..6346
                     /locus_tag="VFC01_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN79991.1"
                     /translation="MAQQILVIGAGITGLATAVALQRRGHDVAVIEERTDMSSGAGIS
                     IWPNALAALDEIGLGDAVRAAGGRVTAGALRWRDGRWLRHPARERLVKALGEPLVVIR
                     RSALTKVLADALADGTVQSGLSARELVSTADGVRVTLSDSSTRTAAAVIGADGTRSVV
                     ARHLNGALADRYVGYTAWRGVAGCTIDPDVAGEVLGSGIEFGHVPLGSDRTYWFATER
                     APEGAAAPQGELDYLKARFASWTDPIPAVLAATDPYDVLRNDLYDRDEARQWSRGPIV
                     AVGDAAHPMRPHLGQGGCQGLEDAAILARFVDATDGLATAFARFSAFRRPRVRALVRE
                     SKLIGQIVNLRPAFLSGLASRATVLGLEALVTRHLAVMAARSAFVLPSDRDTASV"
     gene            6343..6930
                     /locus_tag="VFC01_09945"
     CDS             6343..6930
                     /locus_tag="VFC01_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN79992.1"
                     /translation="MTVTLGPLLVDAAEPRVVEWFWAAALGEPAQRALLSFRPQRRAK
                     TVKNRVHLDVYVRAVEPLLELGATVLADFLPERLTLADVEGNEFCAFLVPGLDDGPPA
                     RAFAVCTDSDLPEELAAWWAERVGAQVHNGPDGTPRWLYGSAGWEGLIWKFVRVADDR
                     VAPNRWQWTTRTDPGVLVDPQGNEFSVRPQDRPAG"
     gene            complement(6902..>7093)
                     /locus_tag="VFC01_09950"
     CDS             complement(6902..>7093)
                     /locus_tag="VFC01_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HZN79993.1"
                     /translation="GLKAWPKCGAQAASPAVWANPTMPTAPTMTTGCAAMPTSGLLGF
                     INPRQICTALLNPLGGLGA"
BASE COUNT         1119 a         2372 c         2405 g         1197 t
ORIGIN      
        1 tcacctcgcc ttcgtccgcg ccgatcttgt tcgcgatgtc ctgcacggga atctgattga
       61 agagatcgtc gagaccagcc atgacctgtc taccttcgtt gctgggatat tgcacggtcg
      121 cgatcacgac attagtgcac gtgatcttgg ttcacaggtg ttcggctacg cccgatcaat
      181 tcagtcggac ggtctccaat gacacaccgg cagcggggaa tcgggccatc agcgcgtcgc
      241 ccatcgcggc cgccggggtg agcacaccgc gcagctccga cagccggtca cggtccaggg
      301 caagcgccaa tccgctttcg cccagcaaaa ccgacgtcgc cttgtagccc gggtcgccct
      361 tctgcgacat ggtggaccgg tagcgcgcgc cagtggttgt cgtcgtgtaa gtctcgaccg
      421 cgtagcggcc ctgttcgcgg gcacgttcgc tggggccggt acccggcttg ggagcaatgc
      481 gttcgacgag cttgttcggc agtcggttga aatagcggcc gccgagcgcc atcgtcgcgg
      541 cgttgccgcc ggtggccatc gcggccgcga gcggggcgag gatcgacttg ccgaggctca
      601 tctgctccgc gtactcgaac cgcttgccgt acgcgtagtc gagtaatgcg ttggagcgcc
      661 ggactattcg agtgttcgcg gcggccatca cgaacgcccc catccagtag ccattgagtt
      721 ctggcgcaat ctcccggccg cgccgccacc gcacgtcgcg ctgcgcaccg agttccggtt
      781 ccgcgccgcg gtcgggggtg agcgtgtaag ggtcgttcat caaccgcctg gcctcgggat
      841 cggaggacgc cgttcgcagc agttcgatca tcgaggcgac agtgccgccg gacaccccgc
      901 cggcgaacgc gcgcaccacc atattggtgt cggtgagctg acccgcgtcg tcctcgatag
      961 cgcgccggta cagcgcgaaa acggtgagat ccgaagggat ggaatcgaat ccgcacgagt
     1021 gcacgatgcg tgcgccggtg tcgatcgcct gcttgtggta caggtcgatg ctttcgcgaa
     1081 tgaagagcgc ttcaccggtc aggtcggcgt agtccgtccc cgcggccgcg cacgctgcaa
     1141 ccagcggcaa tccgtatttg aggtagggcc cgacggcggt caccaccacc tgcgtctggg
     1201 ccgccatcgc atccagtgtc gacggctggg aagcgtcagc ggcgagcagc ggccacgact
     1261 gcgccttctc gcccagcgtg tcccgcacgg cgcgcaactt gtcttgcgac ctgcctgcca
     1321 gcgcgatgcg ggcgtcaccg cccgcccggg ccagatattc ggcggtcaac ttgccgacga
     1381 aaccggtcgc cccgtacagg acgatgtcga attcacgctg cgctgcgctc atgacgccta
     1441 cgctaaccca gcgtcgggaa aggacgacgc ccatgctcga cggcgccatg atcgtctggt
     1501 ttgcgctgac tgcagccagt gtcgccttcg tcgtcgccga ctctgttttc aacggcgtca
     1561 ccagctgggt ccagcgcacc gcgtggatcc tggtcacggc ctacacaggt ccggttggcg
     1621 tcttcttgta tctgctggcc tgccgcaggc cgctgccggg aacccacgac gcattcacgt
     1681 cggcgacgtg gaagcaatcg ctcaacagcg agatgcactg cctggcgggc gatgccaccg
     1741 gcatcgtggt gcggcctcga tcctgccggt gttcggcctc accaacggct gggacgccac
     1801 cgtcgcgatc atgctgatcc tcggcgcaca ttggccgggc tccgaggacc cgcaccatcc
     1861 gtcgttctgg ttccgcatga gcgtcgcgac catcgtcggc ggtgtcgtcg cctatccgat
     1921 caaccactgg ctggtcgcaa agagactcaa gcacggctgc atgacgttac cgggctcaga
     1981 tgcaccggcg ccgggcctgg gccacgtctc gccggagccg tcgatgatga tggatcacgc
     2041 tgcgacggcc caacaatccg agcacacgat gaccgaacta ccggccgctc gcgccgtagc
     2101 gatcatcgcc gggacgttcg caatcctgct cgtcgcggtc tggctgacga atctcgttgt
     2161 tccggtgcgc ttttagttcg ctaaccgagt gcctcgggca gttcgacatc ctcccccaac
     2221 acgtgacggg ccaggaaccc taggacgact tggtaccaca gcttggcatg ctgcggggtg
     2281 agcacccagt ggttctccga cgggaagtag agaaatcggt gcgggctggt gccgtcgtcc
     2341 gaggcgggta ggccggactc ggtgagcagc tcgaaccaca gccgcaacgc ctcgccgatc
     2401 ggcacgcgat agtccttgtc gccgtggatg accagcatcg gggtgcggat ctgcgccacg
     2461 aagcgtcgcg gcgaattctg ttctgccatc tcgggtgtca tctcgcgtgc ccaccagtac
     2521 gcgccgtcgg tggtcgaccc gaactggtcg agcgcccaaa ggctggcatg ggtgacgatc
     2581 gcatcgaacc ggtcggtgtg tccggcgatc cagttggcca tgtaaccacc gaacgagcca
     2641 cccatcaccg cggtgcgcgt ctcgtccacc cgcggatgcg cgcacgccgc gtcggtcgcc
     2701 gcgatgagat cggtgtacgg cgcgaaaccc catgcgcccc agccacgctg gatgaagtcc
     2761 tggccgtagc cggtggacag cgcggggtcg ggcatcagca cggcgtaccc ctcggcgacg
     2821 agcaaccacg gattccaccg ccaatgccag gcattccagc tggccagcgg gccgccgtgc
     2881 acccacagca ccaacggcgc gggtgcatcg ccgtgcggca atgccaacca tgaccggacg
     2941 ggctggccgt cgtccgcgat cgccgccacc tcggtcagcg tgccgggcag tgcgggagcc
     3001 gccacactcg gcagctccgt cacggtcccg tccgggtcga tacgcaccgg atgtggaggc
     3061 cgcgcatacg aactgcgcat cgcgtacacc acgccatcgg gcgcggtgcg cacatcggag
     3121 taggtgtagt cgtcgtcggt cagccgcgtg accgccccgc tcgcagggtc gatcgcgaag
     3181 attgggccgc gcccggcctc gtcggcggtg acgatcaggc tcgagccgtc gcgcgaccat
     3241 gtgaccgatg tcggccaccg gtcccaatca ccggtcagct cgacaaaggg ttcgccgaaa
     3301 tgcatgtgac acaaggtgat tcgcggtgct ttgtcgggtg tggaatgcgt ctcacgggtg
     3361 aatgccaccg cggtgccgtc gggcgagatg gccggggact ccaggtcggc atcgggatca
     3421 tcggcgatcg tggtccgctg cccggtagcg tgatcgattc ggacgagggt gccacggatg
     3481 gacgcgccgg ggccggggcc atgccatgac gtcacgacga aactgccgtc ggagctcatg
     3541 tcgaaatcgg cctcgcgcaa cgcatcaccg ggctggggcg tcaggtcacg cggcccgtcg
     3601 atgtcgagca gatgagggtg gtcggggccg aggtcgtggt cccagtaccg caccggatag
     3661 ccgctgtgca gcacggcgga caccgtgttg tccttgcgca gcgaacgcaa gcgcctgtcc
     3721 tcgtccatac ctgacgctga ccgcagcagc ttcgccgcga ccaccgtgac ggccatcgtc
     3781 cgcgcggcgc gaacgtcctc cacgccaccc ggcagcgcga gcgccacgac ggcttctccg
     3841 cccgcggcgg gcaaccgcca aagggatgcg ggcggcttgt cgtcctcctc ggttggccgc
     3901 gccgcgacga acaaaagatc gccgtcagac gtaaaggccg gcgacgactc accctttgca
     3961 ccgcgggtca ggcgacgtgc cggtcgtcgc ccagccggat cgagttccca gatcgcggtg
     4021 acgaattccg tccgcttgtc gttgagctcc gcgatcgtcg taacgacccg cgacccgtca
     4081 ggtgacaccg cgaggccgga cacccgtggc agcgcaagga actcgtcgag gtcatcaaaa
     4141 ggggtggtcg ccacacccat ttggtagcac accacgcaag tcgatttggc agtgagtttg
     4201 actttcaaac tcactgcgcg atgctggcca catgagcaca gtggtgctgg gtgagcttgc
     4261 aggacgagcg tgtccgatcg ctgcggcgct tgagcttgtc ggcgagcgct gggcgctctt
     4321 agtcgtcagg gagatcgcgc tcggcgcgac ccacttcagc gacatcgtgg cgggtaccgg
     4381 cgcgccgcgt gatcggatcg cagcgcggtt gaaggcactg gaaagcgctg gagtcgtcgt
     4441 ccggtcgccc tatcagagtg cgcccactcg ttacgaatat cggctcacca cgtcgggaga
     4501 agcactcatt ccggtgctcg atgccctctt ggcatggggc aaggtgcacg ccgtctcgcc
     4561 ggacgatccc gatcgacagc agaagtaccg acccttcagc gaactaagga agtctcgatg
     4621 accgcagaac tcgtagatac cgacattcag gccgactggg gcccgcagcg ctcacgcacg
     4681 gtgacctggt attcgccggg gcccagcacc gccaagggcc tgtcgatgtc tggcatcgag
     4741 tacctgcgcg cgatggtcga cggcgacctc ccccaacccc cgatcgccgg actgatgcag
     4801 ttcgatctcg tggaggtgga gccgggacgg gcggtattca cctgtgtccc agacgaatcc
     4861 gcgtataacc cgatcggcgc catccatggc gggctgatct gcacgctgtt ggactcggtg
     4921 accggatgcg cggtgcacac cacgctttca cagggcaagg gatacacgtc ggtcgagatc
     4981 aaagtgaact acctcaaggc cgtgcagctc acgagcgggc tgctcacagc aaccggcacg
     5041 gtggtcaaga ccggttcgcg cgtcggcttc gccgaaggcg tcatcaccga tgccagcgga
     5101 gccgtcgtcg cgacggccac cagcacgctg cttatcttcg atctgtagcg agccgccgcc
     5161 gcgctacgtt cgttggatgg cgcagcagat cctggtcatc ggcgcaggca tcaccgggct
     5221 ggcgaccgcg gtggcattac aacgtcgcgg ccatgacgtc gccgtcatcg aggagcgcac
     5281 cgacatgtcc tccggtgccg gcatcagcat ctggcccaac gcgcttgcgg cactcgatga
     5341 gatcggcctc ggcgacgccg tccgcgcggc gggcggcagg gtgaccgccg gggcgctgcg
     5401 ctggcgcgac ggcagatggc tgcgccatcc ggcccgggag cggttggtca aggcgctcgg
     5461 tgagccgctg gtggtgatcc gccgctccgc actgaccaag gtgctggccg acgcgctcgc
     5521 cgacggcacc gtgcagtctg ggctgtctgc ccgggaactc gtgtcgaccg ctgacggtgt
     5581 gcgggtcacg ctgtcggact cgtcgacgcg gaccgccgcg gctgtgatcg gcgccgacgg
     5641 cacgcggtcg gtggtcgccc gccacctcaa cggcgcgctg gccgaccgct acgtcggcta
     5701 cacggcgtgg cgcggggtcg ccggctgcac gatcgacccc gatgtcgccg gcgaggtgct
     5761 gggttcaggc atcgagttcg gccatgttcc cctcggatcg gatcgcacgt attggttcgc
     5821 caccgagcga gcaccggaag gcgccgccgc gccgcagggc gaattggact acttgaaggc
     5881 ccggttcgcg tcgtggaccg accccatccc ggcggtgctg gccgcgaccg acccctacga
     5941 cgtgctgcga aacgacctct acgaccgcga cgaggcgcgg cagtggtcgc gggggccgat
     6001 cgtcgcggtc ggcgatgcgg cgcaccccat gcgtccccat ctgggtcagg gcgggtgtca
     6061 ggggcttgag gacgcggcga ttctggcgcg gttcgtcgac gcgaccgatg gcctggccac
     6121 ggcgttcgcg cgcttctcgg cattccgccg gccgcgggtg cgcgccctgg tgcgggagtc
     6181 gaagttgatc ggccaaatcg tgaacctgcg gccggcgttc ctcagcggac ttgcgagccg
     6241 tgccaccgtt ctgggccttg aggcgctggt gacgcggcac ctggccgtca tggcggcacg
     6301 gtcggccttc gtgctgccca gcgaccgcga cacggcatcg gtgtgaccgt cacgctgggc
     6361 ccgttgttgg tcgacgcggc tgagccacgg gttgtcgagt ggttctgggc cgccgcgctc
     6421 ggtgagccgg cacagcgcgc gctcttgtcc ttccgcccgc agcggcgagc caagacggtg
     6481 aagaaccggg tgcatctgga cgtctacgtg cgcgccgtcg agccgctctt ggagctgggc
     6541 gcgacggtgt tggcggactt tcttccggag cgcctcacgc ttgcggacgt cgagggcaac
     6601 gagttctgcg cgtttctcgt tccgggcctt gacgacgggc caccggcccg agccttcgcc
     6661 gtctgcaccg acagcgacct gcccgaggag cttgcggcct ggtgggccga gcgtgtcggc
     6721 gcccaggttc acaacggacc cgacgggacg ccacgctggc tctatggctc agcgggctgg
     6781 gagggactca tttggaagtt cgtccgggtg gccgacgacc gcgtggcccc caatcgatgg
     6841 caatggacga ctcggaccga tcccggcgtg cttgtcgacc cgcagggcaa tgagttcagc
     6901 gtcaggcccc aagaccgccc agcgggttga gcagggccgt gcaaatctgg cgcgggttga
     6961 tgaagccgag cagaccgctg gtgggcatcg cggcgcatcc ggtggtcatc gtgggcgctg
     7021 taggcatggt ggggttggcc cacaccgccg ggctggcggc ctgcgcgccg cacttgggcc
     7081 atgccttgag gcc
//
LOCUS       DASRJA010000223         7833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2963678,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000223 DASRJA010000000
VERSION     DASRJA010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2963678"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFC01_09955"
     CDS             <1..547
                     /locus_tag="VFC01_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN79994.1"
                     /translation="VAKLTDAEIAAFAMMLGGAGAETVVKLIGNAAVTFAANPDQWRN
                     LRDDRSKIPYAFEELLRYQAPSQYQLRYCKRDVTLHGTTIPADSIVMLINGSATRDER
                     AFADPDRFDIDRVPSGHNLNFGYGVHSCLGAALARMEGRIALDVMLDLMPDYRVDTAG
                     LRRVAMANVAGWSNVPVQVIR"
     gene            complement(544..1500)
                     /locus_tag="VFC01_09960"
     CDS             complement(544..1500)
                     /locus_tag="VFC01_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN79995.1"
                     /translation="MHRVAVLLLPPVVGFDAAIPPLLFSNATDADGNALYEVVTCALT
                     PGPVAATTGFAVLPAAGPEALETADTVVIPGTRYPPARIDGVLAPEASEVLARIRRGT
                     RLVSICTGAFVLAAAGLLDGRRATTHWKFADDMRRLHPQVLVDENVLFVDDGGVLTSA
                     GLAAGIDLCLHIIRSDHGAQVANAVARYCVVPPWREGGQAQFIDRQVPAPDHFSTAAT
                     REWALEHLGEELTVHRLARHANMSARTFSRRFHEETGQSPGAWVRSRRVDRARELLES
                     RDLPVDQVARLSGLGSGGNLRHHLRRGVGMSPSSYRKSYQGI"
     gene            1540..2859
                     /locus_tag="VFC01_09965"
     CDS             1540..2859
                     /locus_tag="VFC01_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN79996.1"
                     /translation="MPLVGSPRIGDSDSVTLTAARKSTPIGRIHWAWVVAAVSFIAIL
                     GAAGFRSVPGVMMNPLHHEFGWSHGVVGLAMSVNMTLFGLTAPFAAALMDRFGVRPVL
                     TAALLLIATGSALSVTMTASWQLVLLWGVLVGVGTGSISMGFVATVATRWFDARRGLI
                     TGVLTAASATGQLIFLPIVAEVTTRHGWRWASLIVAGAAVSVIPLVVLFMRNYPQDKG
                     LTAYGATTTEPAVVPASSFKAAFDGLRIGARVPAFWLLAASFAICGMTTNGLIGTHFI
                     PAANDHGMPTTVAAGLLATIGILDVAGTVFSGWLTDRVDPRVLLVVYYAGRGLSLLLL
                     PSLLSPHAEPSTWVFVIFYGLDWVATVPPTIVLCRNYFGTRCPVVFGWVFASHQLGAA
                     IAAAGAGRLRDLQGNYDMAFYLAAGLCFVAVILCANVRKVQVSAAVT"
     gene            2938..3657
                     /locus_tag="VFC01_09970"
     CDS             2938..3657
                     /locus_tag="VFC01_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HZN79997.1"
                     /translation="MKTRTSKALGAAAGLAAIVASLPLAVNAYADPPTPETPIPDPQG
                     PGCDAFKTELPNWKSFNSQPVGKVLASIPEISTFSALVSGGANPAVNVVPVLENGPYV
                     VFAPTNDAFAALPQLESIKADPAALTKLDYYHEFLGLLGPDDVKGQRPTQEGTEVKVT
                     GKGGDIKVNDTAKVVCGGIQASNARIYIIDTVLDPARGPEPIVPGSSGTSTTSTTTTT
                     TSTEAPPPPPPGDAHMPPPAG"
     gene            complement(3654..4796)
                     /locus_tag="VFC01_09975"
     CDS             complement(3654..4796)
                     /locus_tag="VFC01_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN79998.1"
                     /translation="MKRLVFEPEHEQLRETARQFLEKECAPHVEKWESERLVDRESYV
                     AAGKYGLIGFNMPEEYGGGGVDDFRFNAVIVEELAKFGPATPGLSLQNDIVGPYFASL
                     STDEQKERWLPGYITGELIGAIAMTEPGAGSDLAGIKTTAVRDGDDWILNGSKTFISA
                     GINGDLVIVVARTDPEAGHKGFSLLVVERGMDGFERGRKLDKMGLHAADTAELHFENV
                     RVPGNNLLGEEGKGFYHLMHNLPSERLSIAIAAIAGARETWRETLQYVKDRKAFGQPI
                     GSFQHNRFLMAEMDTELEIAEQYIDRCLRAAVDKELTAVQASKAKWWCTELGKRVVDN
                     CVQLHGGYGYMNEYKVARDYVDVRIQTIFGGTTEIMKEIIGRDLGL"
     gene            complement(4797..5594)
                     /locus_tag="VFC01_09980"
     CDS             complement(4797..5594)
                     /locus_tag="VFC01_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZN79999.1"
                     /translation="MTTDVTAPAALTERRGNVLIITLNRPEARNAVNSAVSTAVGDAL
                     QAAQDDPEVRAVVITGAGESFCAGADLKAISRRENLFHSDHAEWGFAGYVHHYIDKPT
                     IAAVNGTALGGGSELALASDLVVAEERAKFGLPEVKRGLIAAAGGVFRIVDHLPRKVA
                     MELIFTGEPMSSADALKWGLVNQVVPDGTAVEAALALAERITCNAPLAVWASKRVAMG
                     VDDGVIVGDEPGWTRTMREIGTLIRSEDAKEGPLAFAEKRQPVWKAK"
     gene            complement(5591..6736)
                     /locus_tag="VFC01_09985"
     CDS             complement(5591..6736)
                     /locus_tag="VFC01_09985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZN80000.1"
                     /translation="MAEAVIVEAVRSPVGKRNGGLSGVHPGELSAQVLNGLAERTGID
                     PGIVDDVIWGCVMQAGEQALDIGRTAVLAAGWPESVPGVTVDRQCGSSQQSIHFAAAG
                     VVAGHYDVVVAGGVESMSRTPMGASLANGGHPYPEAFRNRYSQTPNQGIGAEMIAEQW
                     GFDRTMVDQFSLGSHEKAAAAQDSGAFDDQIVGIKDQDGNPVLKDEGIRRGTTIEKMG
                     ELKPAFKEDGVIHAGNSSQISDGSAAILFMSAEKAKSLGLKPIAKVHTATLAGADPVI
                     MLTAPIPATQKALKRSGLKLEDIGVFEVNEAFAPVPLAWLKDIGADEKKLNPNGGAIA
                     LGHPLGGSGARIMTTMLYHMRDKGIQYGLQTMCEGGGQANATIIELL"
     gene            6906..7697
                     /locus_tag="VFC01_09990"
     CDS             6906..7697
                     /locus_tag="VFC01_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN80001.1"
                     /translation="MTRSTPLAPMIGRDAVPASAAVRSPKTAELVAGTLRRMVVDGQL
                     KDGDFLPNEAELMAHFGVSRPTLREAVRVLESERLVEVRRGSRTGARVRVPGPEIVAR
                     PAGLLLELTGATIADVMVAQAGIEPMAARLLAESGKPEAFDELDKMLAEHIPAGWQSG
                     RLAETTGDFHRRMVELSGNATLAIIAGMLHEITVRHTAFVFKERRPVSKADYDKLMRS
                     YARLMKLLRAGDGAAAEAHWRKHLDVARSLLLEGLETVKVRDVMG"
BASE COUNT         1223 a         2622 c         2579 g         1409 t
ORIGIN      
        1 tgtggcgaaa ttgaccgatg ccgaaatcgc cgcgttcgca atgatgctcg gcggtgcggg
       61 tgccgagacg gtggtcaaac tcatcggcaa tgccgcggtc accttcgccg cgaatcccga
      121 ccaatggcgc aacctccgcg acgacaggag caagattccg tacgcgttcg aggaactgct
      181 ccgatatcag gcaccttcgc agtaccagct tcgctattgc aagcgcgacg tcacattgca
      241 cggcacgacg attcccgccg acagcatcgt catgctgatc aacggatcgg cgacgcggga
      301 cgagcgtgcc tttgccgatc ccgatcggtt tgacatcgac cgggttccgt ccgggcacaa
      361 cctcaatttc gggtacggcg tgcatagctg ccttggcgcc gcgctggccc ggatggaggg
      421 ccggatcgcg cttgatgtga tgctcgatct gatgcctgac taccgggtgg acaccgcggg
      481 gctccggcgg gtcgcaatgg cgaacgtggc gggctggtcg aacgtgccgg ttcaggtgat
      541 tcgctaaatt ccttggtagg acttgcggta actcgacggc gacatcccaa caccgcgacg
      601 cagatggtgg cgcaggttgc caccggatcc gaggcctgac agtcgcgcga cttgatcgac
      661 gggcaggtcg cgcgactcca gtagctcacg cgcgcggtcc acgcggcggc tgcgcaccca
      721 tgctccgggc gactgtcccg tctcctcgtg gaaccggcga ctgaacgtgc gcgcgctcat
      781 gttcgcgtgc cgcgccagac gatgtaccgt gagctcctcg ccgagatgct ccagcgccca
      841 ctcgcgcgtc gcggcagtcg agaagtggtc cggcgcaggc acttggcggt cgatgaactg
      901 cgcctggccg ccctctcgcc acggcggcac cacgcagtac ctggcgaccg cgttggccac
      961 ctgtgcgccg tgatcggagc gaatgatgtg caggcacaag tcaattccgg cggccagacc
     1021 cgccgacgtg agcacaccac cgtcgtcgac gaacaacacg ttctcgtcga ccagcacctg
     1081 cggatgcagg cgccgcatat cgtcggcgaa cttccagtgc gtagtcgcgc ggcgcccgtc
     1141 gagcagaccg gccgcggcga gtacgaaagc gccggtgcag atcgacacca gccgggtgcc
     1201 gcgccggatt cgcgccaaca cctcggacgc ctcgggcgcc agcacgccgt cgatgcgggc
     1261 gggcgggtag cgcgtgcccg ggatcacgac ggtgtcggcg gtctccagcg cctcgggccc
     1321 tgctgcgggc agcacggcga agcccgtcgt cgccgcgacc gggcccggcg tcagcgcgca
     1381 cgtcaccacc tcgtagagcg cgttgccgtc ggcgtcggtc gcgttcgaga acagcaacgg
     1441 cggaatcgcg gcgtcgaagc ccaccacagg cggcagaagc aggacggcga cgcggtgcat
     1501 gcggccattt tggcatgttt ttgacgactg gtgtcattca tgccactagt cgggtctcca
     1561 cggatcggcg acagtgattc ggtgacgctc accgccgcac ggaaaagcac gcccattggc
     1621 cgcattcact gggcttgggt ggtggccgcc gtcagcttca tcgcgatcct cggggctgcg
     1681 ggtttccgct cggtaccggg cgtcatgatg aacccgctgc accacgaatt cggctggtca
     1741 cacggggttg tcgggctggc gatgtcggtc aacatgacgt tgttcgggct gaccgcgccg
     1801 ttcgccgccg ccttgatgga ccggttcggc gtgcggccgg tgctgacggc agcgctgctg
     1861 ctgatcgcga cggggtcggc gctcagcgtg acgatgaccg cgagctggca gctggtgctg
     1921 ctgtggggcg tgctcgtcgg tgtcggtacc ggctcgatct cgatgggctt cgtcgcgacc
     1981 gtcgccacgc gctggttcga cgcgcggcgt ggcctcatca ccggcgtgct gaccgcggcg
     2041 agcgcgaccg gacagctgat cttcttgccg atcgtcgccg aggtgaccac ccgccacggt
     2101 tggcgctggg cgtcattgat cgtcgccggc gctgccgtca gcgtcatccc gctcgtggtg
     2161 ctgttcatgc gaaactaccc gcaggacaag ggcttaacgg catacggtgc gaccacaacg
     2221 gaaccggccg tggtgcccgc gtcgagtttc aaggccgcgt tcgacggctt gcgcatcggc
     2281 gcccgagtgc ccgccttctg gctgctggcg gcgagcttcg cgatctgcgg gatgacgacg
     2341 aacgggttga tcggcaccca tttcatcccg gccgccaacg accacggcat gcccaccacg
     2401 gtggcggcgg gtctgctcgc gacgatcggc atcctcgacg tggcaggaac ggtgttctcc
     2461 ggctggctga ccgaccgcgt cgacccgagg gtgctgctgg tggtctacta cgccggccga
     2521 gggctatcac ttctattgtt gccgtcgctg ctctccccgc acgccgagcc aagtacctgg
     2581 gtgttcgtca tcttctacgg cctggattgg gtcgccaccg tgccacccac gatcgtgttg
     2641 tgccgcaatt acttcggcac gcgctgcccc gtcgtctttg gttgggtgtt cgcgtctcac
     2701 cagctcgggg ccgcgatcgc cgctgcgggt gcgggtcggc tgcgggatct gcagggcaac
     2761 tacgacatgg cgttttacct ggcggccggg ctctgcttcg tcgctgtgat cctttgcgca
     2821 aacgtccgga aggtgcaggt cagcgcggct gtaacgtaat cgggcggtcg gagcgacttt
     2881 ggaattgggc gttcgctgcc cttaacatgc gatgcgaccg gactgaggag ccgaaagttg
     2941 aagactcgta ccagcaaggc attgggggcg gccgccgggc tcgccgcgat cgtggcgtcg
     3001 ctgccactcg ccgtcaacgc atacgccgac ccaccgacgc ccgagacgcc gatcccggat
     3061 cctcaggggc cgggctgtga cgcgttcaag acggagctgc ccaactggaa gagcttcaac
     3121 agccagcccg tcggcaaggt gctagccagc atcccggaaa tttcgacgtt cagcgcgttg
     3181 gtctctggtg gtgccaatcc cgccgtcaac gtcgtgcctg tcctggagaa cggcccgtac
     3241 gtcgtgttcg cgcccaccaa cgacgcgttc gccgcactgc cgcagctgga gtccatcaag
     3301 gccgatccgg ccgcgctgac caagctcgat tactaccacg agttcctcgg cctgctcgga
     3361 cccgacgatg tgaagggcca gcggcccacg caggagggca ccgaggtcaa ggtgacgggc
     3421 aagggcggcg acatcaaggt caacgacacc gccaaggtcg tctgcggcgg catccaggcc
     3481 agcaatgccc gcatctacat catcgacacg gtgctggatc ctgcgcgggg tcctgagccg
     3541 atcgttcccg gcagcagtgg cacgtcgacg acaagcacga cgaccaccac cacgtcgacg
     3601 gaggcgccgc cgccaccgcc gccgggcgac gcgcacatgc cgcctccggc gggctagagc
     3661 cctaggtcgc ggccgatgat ctccttcatg atctcggtcg tgccgccgaa gatggtctgg
     3721 atccgcacgt cgacgtagtc gcgggcgacc ttgtactcgt tcatgtagcc gtacccgccg
     3781 tgcagctgca cgcagttgtc aacgacgcgc ttgccgagct cggtgcacca ccacttggcc
     3841 ttggacgcct gcaccgcggt cagctccttg tcgacggcgg cgcgcaggca gcggtcgatg
     3901 tactgctcgg cgatctcgag ctcggtgtcc atctcggcca tcaggaaccg gttgtgctgg
     3961 aagctgccga tcggctgacc gaaggccttg cggtccttga cgtattgcag tgtctcgcgc
     4021 caggtttcac gcgcaccggc gatcgcagcg atcgcgatgg acaaccgctc cgacggcaga
     4081 ttgtgcatca ggtggtagaa gcccttgccc tcctcgccga ggaggttgtt gcccggcacg
     4141 cggacgtttt cgaagtgcag ctcggcggtg tcggcggcgt gcaggcccat cttgtcgagc
     4201 ttgcgtccgc gctcgaagcc gtccatgccg cgttcgacga ccagtagcga gaagcccttg
     4261 tgtcccgcct cggggtcggt gcgggccacg acgatcacca gatcgccgtt gataccggcg
     4321 gagataaacg tcttggagcc gttgaggatc cagtcgtcgc cgtcgcgcac cgccgtcgtc
     4381 ttgatgccgg ccaggtcgct tccggcaccg ggttcggtca tcgcgatcgc gccgatgagt
     4441 tcaccggtga tgtagcccgg cagccaccgc tccttctgct cgtcggtgga cagggatgcg
     4501 aagtatgggc cgacgatgtc gttctgcaga ctcaggccag gggtcgcggg gccgaacttc
     4561 gccagttcct cgacgattac ggcgttgaac cggaagtcgt cgacgcctcc gccgccgtat
     4621 tcctcgggca tgttgaaccc gatgagaccg tatttgcctg cggccacgta cgattcgcgg
     4681 tcgacgaggc gttcgctctc ccacttctcg acgtgcggcg cgcactcctt ttcgaggaac
     4741 tggcgcgcgg tctcccgcag ctgctcgtgc tcgggttcga ataccagtcg cttcatctat
     4801 tttgctttcc aaacgggctg acgcttttcc gcaaacgcca gcggtccttc cttggcgtcc
     4861 tcggaacgaa tcaacgtccc gatttcgcgc atggtcctcg tccagccggg ctcgtcgccc
     4921 acgatcacgc cgtcgtcgac gcccatggcc actcgcttac tagcccacac cgcaagcggt
     4981 gcgttgcagg tgatccgctc ggcgagcgcc agcgccgctt cgaccgcggt gccgtcgggc
     5041 acgacctggt tgaccagtcc ccacttgagt gcgtcggccg agctcatcgg ctcaccggtg
     5101 aagatcagct ccatcgcgac cttacgtggc aagtggtcga cgatgcggaa caccccaccg
     5161 gcggcggcga tcaacccccg cttgacttcg ggcagcccga acttcgcgcg ttcctcagcc
     5221 accaccaaat cactcgcgag cgccagctca gagccgccac ccagggccgt gccgttcacg
     5281 gcggcgatgg tcggcttgtc gatgtagtgg tgcacgtaac ccgcgaaacc ccactcggcg
     5341 tggtcggaat ggaacaggtt ttcccgccgt gatatcgcct tgagatcggc cccggcgcag
     5401 aaggattcac ccgcaccggt gatcaccact gcccgaacct cgggatcgtc ctgcgcggcc
     5461 tgcagcgcgt cacccaccgc cgtgctcacc gcgctgttga ccgcgttacg ggcctcgggc
     5521 cggttcagcg tgatgatgag gacgttcccc cgccgctccg tgagggcggc gggggcggtc
     5581 acatcggtgg tcacagcagc tcgatgatgg tggcgttggc ctggccgcca ccctcacaca
     5641 tggtctgcag accgtactga attcccttgt cccgcatgtg gtacagcatc gtcgtcatga
     5701 tgcgggcgcc ggagccgccg agcgggtgac cgagcgcgat cgcgccgccg ttcggattca
     5761 gcttcttctc gtcggcgccg atgtccttca gccacgccag cggcacgggg gcgaacgctt
     5821 cgttgacctc gaatacgccg atgtcctcga gcttgaggcc agagcgcttc agcgccttct
     5881 gggtggccgg gatcggcgcg gtcagcatga tcaccgggtc ggcaccggcc agcgtggcgg
     5941 tgtgcacctt ggcgattggc ttgagcccca acgacttagc cttctcggcc gacatgaaca
     6001 ggatggcggc cgacccgtcg gagatctgcg aggagttacc ggcgtggatc acaccgtctt
     6061 ccttgaacgc cggcttcagc tcacccatct tctcgatggt ggtgcctcgg cggatgccct
     6121 cgtccttgag caccggattg ccgtcctggt ccttgatgcc gacgatctgg tcgtcgaacg
     6181 caccggaatc ctgtgcggcc gccgccttct cgtgcgagcc caacgagaac tggtccacca
     6241 tggtgcggtc gaagccccac tgctcggcga tcatctcggc gccgatgccc tggttcgggg
     6301 tctggctgta gcggttgcgg aacgcctccg ggtacgggtg cccgccgttg gccagcgacg
     6361 cgcccatcgg cgtgcgcgac atcgactcca caccgcctgc gaccaccacg tcgtagtgtc
     6421 cagcgaccac gccggccgcc gcgaagtgga tggactgctg gctggatccg cactgccggt
     6481 cgacggtcac accgggcacg ctttcgggcc agcccgccgc cagcaccgcg gtgcggccga
     6541 tgtcgagcgc ctgctcgccg gcctgcatga cgcatcccca gatgacgtcg tcgacgatcc
     6601 ccggatcgat gcctgtgcgc tcggcgaggc cgttgagcac ctgggcggac agctcgccgg
     6661 gatgcacacc cgacagcccg ccgttgcgct taccgaccgg cgaccgcacg gcctcgacga
     6721 tgacggcttc agccatgaca cttctccttt gaatgagtgg tgtttccccg aaggtagagc
     6781 aacaaagttt actaggtcaa cctactggtt ggtaggctgg caccggacgt tcatgccgct
     6841 cctagccttc ccgacatggt ttactgagtt gcatagctcg ggggaaacac tgtttgggag
     6901 aggttgtgac gcgaagcaca ccgctggcgc cgatgatcgg ccgtgacgcc gtcccggcga
     6961 gtgcggcggt ccgctcgccg aagacggccg agcttgttgc gggcacactg cgccgcatgg
     7021 ttgttgacgg ccagcttaag gacggcgact tcctgcccaa cgaggccgag ctgatggccc
     7081 acttcggcgt cagccgtcct actttgcgcg aggcggtgcg ggtgctcgag tctgaacgac
     7141 tcgtcgaggt gcgacgcggg tcccgcaccg gcgccagggt ccgggtgccc gggccggaga
     7201 tcgtcgcccg gccggcaggg ctgctgctcg agctgacggg cgcgaccatc gccgacgtca
     7261 tggtggccca ggccggtatc gagccgatgg ccgcgcggct gctcgcagaa tccggcaagc
     7321 cggaggcgtt cgacgaactc gacaagatgc tggccgaaca catccccgcc ggctggcaat
     7381 ccggtcggct ggccgagacg accggagact tccaccggcg catggtggag ttgtcgggca
     7441 atgccacgct cgcgatcatc gccggcatgc tgcatgagat caccgtgagg cacaccgcat
     7501 tcgtcttcaa ggaacgccga ccggtatcga aggccgacta tgacaagctg atgcgctcct
     7561 atgcgcggct gatgaagttg ttgcgcgcgg gcgacggcgc cgcggcagag gctcactggc
     7621 gcaagcacct tgacgtggct cggagcctgc tgttggaggg ccttgagacc gtcaaggtcc
     7681 gcgacgtgat gggctagtcc ccttcgccga aactgcgggg agatcgcgtt ttcgaccgag
     7741 attgcgatct gtgggcagtc tcgaggcgtg tggcccctta gtccggccag accgccaggc
     7801 gcgttggggt gtccaccagc ccgtcctccg tct
//
LOCUS       DASRJA010000224        17661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2408991,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000224 DASRJA010000000
VERSION     DASRJA010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17661
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2408991"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="VFC01_09995"
     CDS             <1..1488
                     /locus_tag="VFC01_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN80002.1"
                     /translation="IHTERPVIGTLLALGARRGRVVRHYVWYGAVVAAVAALAGVLVG
                     GAATTAYTNAYAAILRLPDTVIEHRIPTAVIGFVLGLAAGVVGGLVPAIAAARTAPAE
                     AMRGDVVHPMRMGPLARRSARWTVVPVAVRMALRSLTRSRRRTAATMIGGVLALVLIL
                     ASAGMLTSVRAMLGIEFGWVQRQDASVLVVRGANDVAAQLQSLPGVAVVEPATIARVT
                     VGGNGRTYATSLTGLEPATVMHGFRTPDGTARTLPADGVLAGVALADELGVRVGDNVT
                     VIPATGSVREVRLAGLVDEPLGTTLYATNATAQSMTNAGADGYLIRFDNDADRDRVRA
                     AATGLTGVVAYTDAHAVENQIERFLVIFWAFAGTTLVLGTLLAFTVIYVTMTVNLAER
                     EGELATLRATGAPVGRLTATVAIENLAATLLAVPVGLAAGVGAGWLFLRSFNNDLFNL
                     HLSVGPVVLILATIAVAAAAALSQLPAARLIKRIDVARVVRDRSQ"
     gene            complement(1423..2058)
                     /gene="idi"
                     /locus_tag="VFC01_10000"
     CDS             complement(1423..2058)
                     /gene="idi"
                     /locus_tag="VFC01_10000"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HZN80003.1"
                     /translation="MKPEPDNRVVSSDDEQLILVDSHDREIGYLAKADAHLGRGTLHR
                     AFSLFVFNPAGELLLQQRANGKRLWPGCWSNTCCSHPRRGEKMDTAIRRRLREELGMS
                     ADLEFLFKFHYQTQYDSQGAEHELCWVYAGRSAERPLSNINEVAAWRYIMPQALQAEI
                     ERAPETFTPWFKLEWANIVRAYPQVPARPNLLRSVPDNPGDVDPLDQPGRR"
     gene            complement(2074..3000)
                     /locus_tag="VFC01_10005"
     CDS             complement(2074..3000)
                     /locus_tag="VFC01_10005"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZN80004.1"
                     /translation="MSVCERSGLSMRGRVRTTVSDYLALTKPRTIHLVVGAIPAILLA
                     DRGVVPWLLLLNTLIGLTLMTAGGNTLNCIADADIDKQMKRTAGRPLARGAISTRLAL
                     VFGLALTVGSFVWLLWTTTLTAALLAVATIAFYVFVYTLWIKRRSSHNVVWGGAANAM
                     PVLIGWSAVTGTIAWQALVMFAIIFFWTPPHSWALALRNKEDYQAARVPMLPAVATEC
                     SVTKQIIVYTWLTACATLLLVLATGWLYDAFALFAAAWLLVMAYRLDARVRRGEPAES
                     MGLFKRSNTYLTVVFCAVAVDSSIALPTLLGH"
     gene            complement(3355..4218)
                     /locus_tag="VFC01_10010"
     CDS             complement(3355..4218)
                     /locus_tag="VFC01_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZN80005.1"
                     /translation="MVRPQEPVVTGDAVVLDVQIAQLPVRALSALIDITVVFIAYIIG
                     VLLWASTLAQFDTAFSAAVLIIFTVLSLVGYPVAFETATRGRSLGKMALGLRVVSEDG
                     GPERFRQALFRALAGVIEIWILSGGPAVICSLLSPKGKRIGDIFAGTVVISERAPRLT
                     PPPPMPPQLAWWASSLQLSGLGPEQAELARQFLSRASQLDPGVRDQMAYRIAGEVVAR
                     ISPPPPPGVPPQLVLAAVLAERHRRELARLRPPPPVAPPPQWPAQPAYQQPAPPVAPS
                     PDLDGGFTPPS"
     gene            4247..5239
                     /locus_tag="VFC01_10015"
     CDS             4247..5239
                     /locus_tag="VFC01_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HZN80006.1"
                     /translation="MDVDAFVLAHRPTWERLEALVKRRRRLTGAEVDELVDLYQRVST
                     HLSMVRSASTDSVLVGRLSGLVAQARSVVTGAHAPLWSEFVRFWTVSFPVVAYRSWRW
                     WLGTAVAFFFVVVLVTLWVSGNHELQSSLKTPSEIEELVNRDFASYYSEHPAGSFALQ
                     VWVNNSWVSVQCIAFAILLGIPIPFLLLQNALNLGVIAAFMFDAGKADVFLGLLTPHG
                     LLELTAVFLAGSAGMRLGWSVVSPGNRPRGQALAEEGRAVVAVAVGLIGVLLVAGLIE
                     ALVTPSPLPTVVRIGIGAAAEIAFLAYVFHFGRKAAKAGETGDVDDAPDVVPTS"
     gene            complement(5236..6558)
                     /locus_tag="VFC01_10020"
     CDS             complement(5236..6558)
                     /locus_tag="VFC01_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN80007.1"
                     /translation="MILTGRVGLIALICVLPIAFSPWPATTFAVLLGALVTAVIADMA
                     LAANTRRLTCSRFGETAARLGQPVDAVLAVENTSGSRFRGQIRDAWPPSARAEPRTHA
                     VDLAAGQQVRLVTQLRPVRRGDQSSALVTARSVGPLGLAGRQSSHRVPWQVRILPPFL
                     SRKHLPSRLAKLRELEGMTPVLIRGHGTEFDSLREYVIGDDVRSIDWRATARRGDVAV
                     RTWRPERDRRVVIVLDTGRTSAGRVGVDPTASDPGGWPRLDWSMDAALLLAALAARAG
                     DHVDFIAHDRLTRAGVYNASRTELLAQLVDAMAPLQPALVESDARALVAAVRRRIRRS
                     ALVVLLTDLNASALDEGLMAVLPQLTAKHQVLVAAVADPRVDRLAAGRADAAEVYDAA
                     AAERARNDRRTIASRLRRSGVDVVDAPPEELAPELADHYLALKASGRL"
     gene            complement(6558..7535)
                     /locus_tag="VFC01_10025"
     CDS             complement(6558..7535)
                     /locus_tag="VFC01_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN80008.1"
                     /translation="MTQPTAQETPTSISARNALLALRGEIAKAVVGQDAVVSGLVIAL
                     LCRGHVLLEGVPGVAKTLLVRTLAAALQLEFKRVQFTPDLMPGDVTGSLVYDARTAEF
                     EFHSGPVFTNLMLADEINRTPPKTQAALLEAMEERQVSVEGEPRPLPDPFIVAATQNP
                     IEYEGTYQLPEAQLDRFLLKLNVPLPPRDQEIAILDRHARGFDPRDLSAVKPVAGTTE
                     LAAGREAVRQVLVADEVLGYIVDIVGATRHSPSLQLGVSPRGATALLATARSWAWLSG
                     RNYVTPDDVKAMARPTLRHRISLRPEAELEGANPDGVLDGILAAVPVPR"
     gene            complement(7532..8683)
                     /locus_tag="VFC01_10030"
     CDS             complement(7532..8683)
                     /locus_tag="VFC01_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HZN80009.1"
                     /translation="MTLSDTAVGSTVSQRWRSVRWVLLALAVIVGVAVLSAYLTAPRP
                     GGPMDPESTSPEGARALVTILREHGVDVVAAGDIAAVENAARPDTLIVVAQTFYLYDD
                     DLLRRLEALPGDRLLVAPVSRAREELAPELRLDGSTTFGDSEPDCDLREANRAGKVQF
                     GLADAYEAASDVPVTRCYGGALVRYTHEGREVTVVSTADFMTNSGLLKQGNAALAMNL
                     AGSHPRVIWYAPQHSETEGETGGGAGLSDFIPNQVGWIVLQLCLVVALLAVWKGRRIG
                     PLVAEPLPVVVRASETVEGRGRLYRSRRARDRAAEALRTAALQRMTPRVGLGNDAEPP
                     AVVTAVAERCGMSPQAVAHILFGPPPASDPDLVNLAHQLDTIERQVAHS"
     gene            complement(8680..9333)
                     /locus_tag="VFC01_10035"
     CDS             complement(8680..9333)
                     /locus_tag="VFC01_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HZN80010.1"
                     /translation="MPTIDIDRDAAHEAAQRELGKPIYPKASLTERLMDWINDMLYRL
                     ADAGASLPGGWLTLSVLFILLAIAIVVAVRIARRAMRTNRGGGRALFGEHQLSAAEHR
                     ATAEQYAAAGNWSAAIRHRLRAVARQLEENGILDPVPGRTATELARDAGRVIPNLSGE
                     LRRAADAFNDVTYGERPGTESAYRMIAELDDHLRSRTSASGAVDVLPTAADGWAEVR"
     gene            complement(9335..10624)
                     /locus_tag="VFC01_10040"
     CDS             complement(9335..10624)
                     /locus_tag="VFC01_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80011.1"
                     /translation="MVNMSNDAGEFQPAGPPPPGYPPPGYPSPGYQDDPQAAYQGYPP
                     PGGYPPPGYPPPGYAPPPGYGGPPPGYGPFGGYPPPGYPPPGYPPPGYGFPPGYGPPP
                     GVQPFKPGVIPLRPLTLSDIFNGAVAYIRANPKATLGLTTVVVVAAQLLALILSVGPL
                     AITGGLQPTLAGEEVSTGVLLSSSASSFAGAIATWLSSILLSGMLTVVIGRAVFGAHI
                     TIGEAWRRLRPRLWALIGFTVLEGIGVAVIIAVVLLIIAGVGAAAGGVAAVVIAVPLV
                     LAMFAGLVYLVTVLSFTPSVIVLERLAIIPAMSRSYKLVRNDFWRVFGIWLLGGIVAA
                     VVAGAVSVPFSFGGQILLISASSTLATLASLVVMSIGSAISQIITAPFSSGVVVLLYT
                     DRRIRGEAFDLVLQTGAAFGPGAPADSTDHLWLTRQA"
     gene            10677..11141
                     /locus_tag="VFC01_10045"
     CDS             10677..11141
                     /locus_tag="VFC01_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZN80012.1"
                     /translation="MAELKDRLRADLTTAMKSQDKLRTATLRMVLAAIQTEEVAGKAS
                     RELSDSEVIKVLSREAKKRSESAEIYTQNGRGELAANEHAEGRIIDEYLPTPLTEAEL
                     ADVADTAIAQVAEDIGERPSMRQMGQVMKAATAIAAGKADGARLSAAVKARL"
     gene            11178..12164
                     /locus_tag="VFC01_10050"
     CDS             11178..12164
                     /locus_tag="VFC01_10050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN80013.1"
                     /translation="MTRFGYTLMTEQSGPKDLVRYAVSAERAGFDFEVSSDHYFPWLS
                     SQGHAPYAWSVLGAVAHATEQVELFTYVTCPTMRYHPAVVAQKAATLQILADGRFTLG
                     VGSGENLNEHVVGGRWPTVSRRQQMLKEAIQIIRELFTGELVDWKGEYYEVDSARIWD
                     VPETPVAIATAVSGERSVDMFAPLSDHLIAVEPNKEIVDAWQNARRATGLPGEVRVIG
                     QIPICWDPDRDAAVQRAHDQFRWFGGGWAVNADLPTTAGFEGATQFVRPEDVAESIPC
                     GPDLDAIVEAVSKYWEAGFTDIALVQIGDDSQELFLKEAAGSLLEKLRTESD"
     gene            12177..12329
                     /locus_tag="VFC01_10055"
     CDS             12177..12329
                     /locus_tag="VFC01_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80014.1"
                     /translation="MPRGKGIYDDEDADEPKGGRPGPADEGRDGGMATRENAADVAET
                     GGEPTA"
     gene            complement(12333..12923)
                     /locus_tag="VFC01_10060"
     CDS             complement(12333..12923)
                     /locus_tag="VFC01_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HZN80015.1"
                     /translation="MLAEGILERIAPPNEGLPMQSCDVIGRVSKGIGLPRALPDIAGL
                     AWRIPPPQDLRSCVPWDVLLASTIANSRIVLAPTRSWSKTSFSCLMPLRFHGDAWWVR
                     ARLVTKIDEPGLSLDTIRNQIDSGAIDFDIDQAKGTGGFSPLARLSLRHLDPSNDDIA
                     FDPVLHSDEDVRLVPGWLADFRRAAYRRSREGRESD"
     gene            13132..13827
                     /locus_tag="VFC01_10065"
     CDS             13132..13827
                     /locus_tag="VFC01_10065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HZN80016.1"
                     /translation="MLTTAYTDLDIPAVTEGVYVPQEDTQLLIDIMDKTGLSRGHRVA
                     DLCTGSGVVAIAAYEQGAAEVTAFDICPRAVHCARANSAAAGVDVAVHLGSWARSTEF
                     GPYDLVVCNPPYVPRDPNDEEIMLPSRLGPAWAWDAGYDGRLVLDPLCAAASDLLADG
                     GTMLLVQSEFAAPRQTLVALASSGLDTEIVAWQWIPFGPVLSSRAEWLEDTGRLEPGR
                     REEELLVIRADKP"
     gene            13824..14042
                     /locus_tag="VFC01_10070"
     CDS             13824..14042
                     /locus_tag="VFC01_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HZN80017.1"
                     /translation="MRDDKPRMVRVVPSGPVMVEGPVRIEMPDGTVVESDRFMVAICT
                     CRRSKSYPLCDTSHRCRQRPERSGQRPA"
     gene            complement(14022..15041)
                     /locus_tag="VFC01_10075"
     CDS             complement(14022..15041)
                     /locus_tag="VFC01_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HZN80018.1"
                     /translation="MTLPSTSVEPTLPRAVGPLSSAVIDTLARGRPALHARPIDVPLC
                     EADPYGLDLQLALYVCYELHYRGFEGVNPRWEWNPGLLHVRGRLEETFLSAVRNDVGE
                     ISRDETAADAMDALSMEPVDGTGPSYYLRDTGTWEQMREYFVQRSLYHLKEADPHAFA
                     IPRLIGQSKASFVAVEFDEYGGGRGALVHQQLFADLLDAAGLDSTYLAYLDAVPADAL
                     ASVNLMSLFGLHRELRGAAIGHFASTEITSSPGSRRLVDALERMGAPEACIGFYREHI
                     EADAVHEQVVRTDVVGDLVAREPHLDRDVVFGIKARDVVEYRLAAHVMNCWRAGRSSL
                     RRPLA"
     gene            complement(15072..15362)
                     /locus_tag="VFC01_10080"
     CDS             complement(15072..15362)
                     /locus_tag="VFC01_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80019.1"
                     /translation="MTTDLRYDRWFLPFSVPFGLGPKHSAIGVEDDKLHVRFGWRFRT
                     EIALASIKDAKPNNDRVYARAYVMGVPIKLRTLYVSVTNPQELIEACAPTVV"
     gene            15451..15696
                     /locus_tag="VFC01_10085"
     CDS             15451..15696
                     /locus_tag="VFC01_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80020.1"
                     /translation="MVGTLLVIYVIGLLPVSCAAFAASKRFADRRAPPDLPTAVMLSL
                     VAGAVWPLLVVGLIELSSVMMLFKAPSKASGGVGIFA"
     gene            complement(15796..15869)
                     /locus_tag="VFC01_10090"
     tRNA            complement(15796..15869)
                     /locus_tag="VFC01_10090"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15833..15835),aa:Pro,seq:cgg)
     gene            complement(15950..16213)
                     /locus_tag="VFC01_10095"
     CDS             complement(15950..16213)
                     /locus_tag="VFC01_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZN80021.1"
                     /translation="MSIYEIAPTATTFLAEEGQRWGHYGWHPWFLVARLVFLALVITA
                     VWLIVRAYRGNHGIRTLRDAYAKGEVNEAEYRERLAVLRETRR"
     gene            complement(16289..16960)
                     /locus_tag="VFC01_10100"
     CDS             complement(16289..16960)
                     /locus_tag="VFC01_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN80022.1"
                     /translation="MTIRVVVADDQALVRAGFVALLNAQDGITVVAEADTGTAALAAA
                     RELRPDVVLMDIRMPEMDGLAATRAIAADPALATVRVVVLTTFELDEYVFEAMRAGAS
                     GFLVKHTEPAELVRAVRVVADGEALLSPSVTRRLVAEFAARTKPPSGASTALAELTSR
                     EREVMALIAEGLTNAEIGERLFMSPATARTHVSRILTKLGARDRTQLVVMAYESGLVR
                     PGWRG"
     gene            complement(16957..>17661)
                     /locus_tag="VFC01_10105"
     CDS             complement(16957..>17661)
                     /locus_tag="VFC01_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015890358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZN80023.1"
                     /translation="QRRAVLDARRQRRAAMARETQLEQERRATQARLSIARELHDVLA
                     HSLSMINVQSSVALELLDDQPERAGPALAAIKDASRQAISDVHSLVTALRADPGQPTA
                     PTPGIADLDRLVGTARATGLTVTTAVQGEPRPLPTVIDVAAARIVQESLTNVVRHSTA
                     ENAEVTVTYGADELAVAVGNDGRPLNAGPSSGGSGITGMRERARALGGDLSVRRHPGG
                     GFSVRATLPVESDCES"
BASE COUNT         3065 a         5874 c         5857 g         2865 t
ORIGIN      
        1 atccacaccg aacgaccggt catcggcacc ctccttgccc tgggcgcccg gcggggcaga
       61 gtcgtccggc actatgtgtg gtacggcgcg gtcgtcgccg ccgtcgctgc gctggctggt
      121 gtcctggtcg ggggggcggc gacgaccgcc tacaccaacg cctacgcggc catcctgcgc
      181 ctcccggaca cggtgatcga gcaccgaatc cccaccgccg tcatcggttt cgtgctcggg
      241 ctggccgcag gcgtggtcgg ggggctcgta cccgcgatcg ccgccgcacg aaccgcaccc
      301 gccgaggcga tgcgcggcga cgttgtccac ccgatgcgga tgggtccgtt ggcccggcgc
      361 tccgcccggt ggacggtagt gcccgtcgcg gtgcggatgg cgctgcgttc cctgacccgc
      421 agccgacgcc gaaccgctgc gaccatgatc ggcggcgtgc tggcgctggt tctgatcctc
      481 gcctcggctg gcatgctcac cagcgtgcgc gcgatgctcg gcatcgaatt cgggtgggtc
      541 caacgtcagg atgcgagcgt gctggtcgtg cggggagcga acgatgttgc cgcacagttg
      601 caatcgcttc ccggcgtggc cgtcgtcgag ccggcaacca tcgcacgggt taccgtcggc
      661 gggaacgggc gcacgtacgc gacgtcactc accggcctcg aacccgcgac ggtcatgcac
      721 ggattccgca ccccggacgg caccgcgcgg acgttgccag ccgacggcgt cctcgcgggc
      781 gtcgcgctcg ccgacgagct gggcgtgcgc gtcggtgaca acgtgaccgt catccccgcg
      841 acaggatccg ttcgggaggt tcgcctggcc gggttggtgg acgagccgct cggcaccacc
      901 ctgtacgcca caaacgccac cgcccaatcg atgacgaacg ccggagctga cggatacctg
      961 attcgcttcg acaacgatgc cgaccgcgat cgagtgcgcg ccgctgccac cggcctcacc
     1021 ggcgtggtcg cctacaccga cgcccacgcg gtcgagaacc agatcgaacg cttcctcgtc
     1081 attttctggg cgttcgccgg caccacactg gtgctgggca ccctgctcgc cttcaccgtc
     1141 atctacgtca ccatgaccgt caacctggcc gaacgggaag gcgaactcgc cactctgcgt
     1201 gcaaccggcg cacctgtcgg ccgactcacc gccacggtgg ctatcgagaa cctcgctgcg
     1261 accctgctcg ccgtcccggt cggcctggcc gctggcgtcg gcgctggatg gctgttcctg
     1321 cgctcgttca acaacgacct gttcaacctt cacctgtccg tcggcccggt agtactgatc
     1381 ctcgccacga tcgccgtcgc ggcggccgct gctctttcgc agctaccggc ggcccggctg
     1441 atcaagcgga tcgacgtcgc cagggttgtc agggaccgat cgcaatagat tcggtcgtgc
     1501 cggcacctgc gggtaggcac gcacgatgtt cgcccattca agcttgaacc acggcgtgaa
     1561 ggtttcgggc gcgcgttcga tctcggcttg cagtgcctgc ggcatgatgt agcgccaggc
     1621 cgcgacctcg ttgatgttcg aaagcggccg ctcggcgctg cgccccgcgt agacccagca
     1681 cagctcgtgc tcggcgccct gggagtcgta ctgcgtctgg tagtggaact tgaacaggaa
     1741 ctccaggtcc gcgctcatgc cgagttcctc ccgcaggcgg cggcgaatgg cggtgtccat
     1801 cttctcgccg cggcgtggat gcgagcagca cgtgttggac caacagcccg gccacaggcg
     1861 tttaccgttc gcgcgctgct gcaggagcag ttcgcccgcc ggattgaaca cgaacagcga
     1921 gaacgcgcgg tgcagcgttc cgcggccgag gtgtgcatcg gccttcgcca ggtaaccgat
     1981 ctcgcggtcg tggctgtcca ccagtatcag ctgttcatcg tcgctggaga cgactcggtt
     2041 atctggttcc ggcttcaagt tgcacctagc cggtcagtgc ccgagaagcg tcggcagcgc
     2101 gatcgacgaa tccaccgcga ccgcgcagaa caccacggtc aggtaggtgt tggaccgctt
     2161 gaacagcccc atcgattcgg ctggctcgcc gcgacgcacc cgggcgtcga gtcgataagc
     2221 catcaccaac agccacgccg cggcgaacag cgcgaacgcg tcgtacagcc agccggtggc
     2281 aagcacgagc agaagagtcg cgcatgcggt caaccaggtg tagacgatga tctgtttggt
     2341 gacgctgcat tcggttgcca cggccggcag catcggcacg cgtgcggcct ggtagtcctc
     2401 cttgttgcgc aacgccaagg cccagctgtg cggtggtgtc cagaaaaaga tgatcgcgaa
     2461 catcaccagg gcctgccacg cgatggtgcc ggtgaccgcc gaccagccga tcaagaccgg
     2521 catggcgttg gcggcgccgc cccagaccac gttgtgcgag ctgcgccgct tgatccacag
     2581 cgtgtagacg aacacgtaga acgcgatcgt ggccactgcc aacagggccg ccgtcagggt
     2641 cgtggtccac aacaaccaca cgaacgaccc gacagtgaga gccaatccga acacgagcgc
     2701 aagacgggtc gagatcgcac cgcgcgccag cggccgaccc gcggtgcgct tcatctgctt
     2761 gtcgatatcg gcgtcagcga tgcagttgag cgtgttgcct cccgcggtca tgagcgtcaa
     2821 gccgatcagt gtgttgagga gcagcagcca aggaacgacg ccgcgatcgg caagcaggat
     2881 cgcgggaatc gcgcccacca cgaggtggat ggtccgcggc ttggttaggg ccaggtagtc
     2941 cgacaccgtg gtgcggacgc gaccacgcat cgacaggcca gaccgctcgc aaacgctcac
     3001 ttcggtactc cacgcctccc cgacatttgg gacacgaatg gcaagcaact tccgttcaac
     3061 gagtcgctta agtcgccgcc ctacgcacgt cgacggacac gacttgccgc ggccgacgag
     3121 ccactgcact caggaccgat ctgggaggaa cctagcgcta gtacacagcc gttgccctgc
     3181 aaggcttctg acgcagacca acacctccgc ggcgctgctt gacatcgaaa ccaagtcgcc
     3241 tgacgtgaag gtcaatttcg agcctcagct gacggggaag ggtaacagtg cggcgagaag
     3301 aacgtgcatg atccgtcctc ggtgtcaccg ccaccgaagt ttctcttcgc ccggctaact
     3361 cggcggcgtg aaaccgccgt cgaggtcggg tgagggcgct accggcggcg ccggctgctg
     3421 atacgcgggc tgcgccggcc actgcggcgg tggagcgacc ggtggtggcg gccgcagccg
     3481 agccagttcc cggcggtgcc gctccgccag caccgcggct agcaccagtt gcggcggtac
     3541 acccggtggg ggcggcggcg aaatccgagc gacgacctcg ccggcgatcc ggtaggccat
     3601 ctgatctcga acaccaggat ccaattgcga tgcgcgggac aggaactggc gggccagctc
     3661 ggcctgctcg ggaccgaggc cggacagctg aagcgacgac gcccaccaag cgagctgcgg
     3721 cggcatcggc ggcggtgggg tcagccgcgg cgcccgctca ctgatcacca cggtgcctgc
     3781 gaaaatatcg ccgatgcgtt ttcccttggg cgacaacaaa ctacagatca ccgccggacc
     3841 gccggagagg atccagatct cgatcacgcc ggccagcgca cgaaagagcg cttggcggaa
     3901 ccgttccggg ccgccgtcct ccgaaaccac acgtagcccc agcgccatct tgccgagtga
     3961 tcggcccctg gtcgcggtct cgaacgcaac cgggtagccc accaaggaca gcacagtgaa
     4021 gatgatcagc accgcggccg agaacgcggt gtcgaactgc gcgagcgtgc tcgcccacaa
     4081 cagcacgcca atgatgtagg cgatgaacac cacggtgata tcgatgagcg ccgacagcgc
     4141 gcgcaccggt agttgggcga tctgaacatc caggaccacc gcgtccccgg tcaccactgg
     4201 ttcttgcggt cggaccatag caatcaacgc tactaggatt tgccatgtgg atgtcgacgc
     4261 cttcgtgctg gcgcatcggc cgacgtggga gcggctcgag gcgctggtga agcggcgtcg
     4321 gcgattgact ggcgcggagg tcgacgagct ggtcgatctc taccagcggg tgtcgacgca
     4381 tctgtcgatg gtccgtagcg catcgaccga ttcagtcctc gtgggccgat tgtcgggact
     4441 ggtggcacag gcgcgttcgg tggtgaccgg tgcgcacgca ccgctgtgga gtgagttcgt
     4501 ccggttttgg acggtatcgt tcccggtggt cgcctaccgg tcctggcggt ggtggctcgg
     4561 cacggcggtg gcgttcttct tcgtggtcgt actggtcacg ctatgggtgt caggcaacca
     4621 cgaactgcag tccagcctca aaaccccaag cgagatcgag gaattggtca atcgcgactt
     4681 cgcgtcctac tacagcgagc accccgcagg gtcgttcgcg ttgcaggtgt gggtgaacaa
     4741 ctcctgggtt tcggtccagt gcatcgcatt cgcgatcctg ctgggcatcc cgatcccgtt
     4801 cttgcttctc cagaacgcgc tcaatctcgg cgtgatcgcg gcgttcatgt tcgacgcggg
     4861 caaggctgac gtcttcctag gtctgctcac cccgcacgga ctgctcgaac tgacggcggt
     4921 gttcctggcc ggctctgcag gcatgcggct gggctggtcg gtcgtgtcgc ccgggaaccg
     4981 gccgcgtggg caggcactcg ccgaagaggg cagggcagtg gtggcggtgg ccgtggggct
     5041 gatcggtgtg ctgctggtgg cgggcttgat cgaggcattg gtgacgccgt cgccgctgcc
     5101 gacggtcgtg cgcatcggca tcggggcagc agccgaaatc gccttcctcg cttatgtttt
     5161 ccacttcggc cgcaaggccg ccaaggcggg cgaaaccggc gacgtcgacg acgcccccga
     5221 cgtcgtcccc acaagctaaa gccggccgct ggccttcagc gccagatagt ggtcggcgag
     5281 ttcgggcgcc agttcttcgg gcggggcatc caccacgtcg acgcccgacc gccgcagccg
     5341 tgacgcgatc gtgcgccggt cgttgcgcgc ccgttcggcc gcggcggcgt cgtacacctc
     5401 ggccgcatcg gcgcgacccg cggcaaggcg gtcgacacgg ggatcggcga cggccgcgac
     5461 gagcacctga tgcttggcgg tcagctgcgg cagcaccgcc atcagccctt cgtcgagcgc
     5521 tgacgcgttc agatcggtca gcaagaccac gagggccgat cgccggatcc gccgccgcac
     5581 tgcggccacc aacgcccgcg catcggactc caccagcgcg ggctgcagcg gcgccatcgc
     5641 gtccacgagc tgggcaagga gctccgtgcg tgacgcgttg taaacaccgg cgcgggtcag
     5701 ccggtcatgc gcgatgaagt cgacgtggtc gcccgcacgt gcggcgagcg cggccaacag
     5761 cagtgccgcg tccatcgacc aatccagccg cggccagccg ccgggatcac tggcagtcgg
     5821 gtcgaccccg acgcggcctg ccgacgtgcg gccggtgtcg agcacgatga ccacccgacg
     5881 gtcccgctcg ggccgccatg tccgcaccgc gacgtcaccg cgccgggcgg tggcgcgcca
     5941 gtcgatggat cggacgtcat cgccgatgac gtattcgcgc agcgaatcga attcggtgcc
     6001 gtggccgcga atcaataccg gtgtcatgcc ttcgagctcc cgcagcttcg ccagccgcga
     6061 cggtaagtgc ttgcgcgaca ggaacggcgg caggatccgg acttgccacg gcacccgatg
     6121 cgagctctgc ctgcctgcca gacccaacgg accgaccgag cgtgcagtga caagcgcgga
     6181 actctgatcg ccgcgccgaa ccggccgcaa ctgcgtgacg agtcgaactt gttggcccgc
     6241 agcgagatcg actgcatgcg tgcgtggctc ggcccgggcg ctcggcggcc aggcgtcacg
     6301 gatctgtccg cgaaatcggc tgccgctggt gttctcgacc gccaataccg cgtcgacagg
     6361 ctggcccagc cgcgcggcag tttccccgaa ccggctgcag gtcagcctgc gtgtgttggc
     6421 cgccagcgcc atgtccgcga tgacagccgt gaccagcgcc cccagcagga ctgcgaaagt
     6481 ggttgccggc cagggggaaa acgcaatcgg cagtacgcat atcagcgcga tcaggccgac
     6541 gcggccagta aggatcacta gcgcggcacc ggcaccgcgg cgaggatgcc atcgagcaca
     6601 ccgtcagggt tggcgccttc gagttcggct tccggccgaa gcgagatccg gtgccgcagg
     6661 gtcgggcgtg ccatcgcctt gacgtcgtcg ggtgtgacgt aattgcggcc cgacagccag
     6721 gcccacgacc gggcggtggc caacagtgcc gtcgcgccgc gcggcgacac gcccagctgt
     6781 agcgacggtg agtgccgcgt cgccccgacg atgtcgacga tgtagccgag cacctcgtcg
     6841 gccaccagga cctgacgcac cgcctcccgg ccagcggcca actctgtagt gcccgcaacg
     6901 ggtttcaccg cggagaggtc acgaggatcg aacccgcggg cgtggcggtc caggatcgcg
     6961 atctcctgat cccgcggcgg cagcggcaca ttgagcttca gcaagaatcg gtccagctgg
     7021 gcctcgggta gttgataggt gccctcgtac tcgatcgggt tctgagtggc agcgacgatg
     7081 aacgggtcag gaaggggacg tggctcccct tccacgctga cctgacgctc ctccatcgcc
     7141 tccagcagag ccgcctgcgt ttttggtggc gtccggttga tctcgtcggc cagcatcaga
     7201 ttggtgaaca ccggaccgga atgaaactcg aattctgctg tgcgggcgtc gtataccagc
     7261 gagccagtga cgtcacccgg catcagatca ggagtgaact gcacccgttt gaactcaagc
     7321 tgcagcgccg ccgcgagcgt ccgaaccagc agcgtcttgg caacgcccgg aacgccctcc
     7381 agcagcacgt ggccgcggca gagcagggcg atcaccagac cgctgacgac ggcgtcctgc
     7441 cccaccacag ccttggcgat ctcaccgcgc aaggccagca gggcatttcg cgccgagatt
     7501 gaagtcggcg tctcttgtgc tgttggctga gtcacgagtg agcgacctgc ctttcgatgg
     7561 tgtcgagttg gtgagcgagg ttgaccaaat cggggtcgct ggccggcggc gggccgaaca
     7621 gtatgtgcgc caccgcctgc ggggacatcc cgcatcgctc ggcgaccgcc gtcaccactg
     7681 ccggaggttc cgcgtcgttt ccgagaccga cgcgcggcgt catccgctgc agtgcagccg
     7741 ttcgcagcgc ctcggccgcg cggtcacgtg cccgccgtga ccggtacaac cgtccgcggc
     7801 cctcgacggt ctcggaggcc cggaccacga caggcaacgg ttcggcgacc agcgggccga
     7861 tacgtcggcc cttccacacc gccaacagcg cgacgacgag gcacagctga agcacgatcc
     7921 atccgacctg gttcggaatg aaatcggaaa gtcctgcgcc gccgccggtt tcaccctccg
     7981 tttcgctgtg ctgcggggcg taccagatca cccgtggatg tgagcccgcg aggttcatcg
     8041 cgagcgcggc gttcccttgt ttgagcagac cggagttggt catgaagtcg gcggtgctga
     8101 cgaccgtcac ctcacggccc tcgtgggtgt agcggaccag cgcgccgccg tagcagcggg
     8161 tgaccggcac gtcgctggcg gcttcgtagg cgtctgctag gccgaactgc accttgcccg
     8221 cacgattcgc ctcccgcaag tcgcagtcgg gctcggagtc accgaacgtg gtgctgccgt
     8281 cgagacggag ctccggtgcg agctcttccc gggcccgaga caccggcgcc accaacaacc
     8341 ggtcgccggg gagtgcctcg agccgacgca acaggtcgtc gtcgtacaga tagaacgtct
     8401 gcgcgacaac gatcagggtg tcgggccgtg ccgcgttctc caccgcggcg atgtcaccgg
     8461 ccgcgaccac gtcgacgccg tgctcacgca ggatcgtgac gagggctcga gcaccttcgg
     8521 gtgacgtcga ttccggatcc atcgggcctc cgggccgcgg ggcagtgagg tacgcgctca
     8581 acacggctac cccgacgatg acagccaacg caagcaacac ccaccggacg ctgcgccacc
     8641 gctgcgagac ggtcgaccct acggccgtat cggaaagcgt catcgcactt ccgcccagcc
     8701 gtcagcggcg gtgggcaaca cgtcgacggc gccggacgcc gacgtgcgcg accgcagatg
     8761 gtcgtcgagc tcggcgatca tccgatacgc agattctgtt cccggccgct cgccgtaggt
     8821 gacgtcgttg aaagcatctg cggcccttcg taattcgccg gacaggttcg gtatgacccg
     8881 tccggcgtcg cgtgccagct cagtcgcggt tcggccgggg accgggtcga ggatgccgtt
     8941 ctcctccagt tggcgagcga cggctctgag ccggtgacgg atcgcggccg accaattacc
     9001 cgccgccgca tactgttcgg cggtggcgcg gtgctcggcc gcgctgagct ggtgttcacc
     9061 gaacaacgct cttccgccgc cgcggttggt gcgcatggcc cgtcgggcga tccgaaccgc
     9121 caccacgata gcgatcgcca gcaggatgaa cagcacactg agcgtcagcc aaccgccggg
     9181 cagcgacgcg ccggcgtccg ccaacctgta cagcatgtcg ttgatccagt ccatgaggcg
     9241 ttctgtcagc gaagccttgg ggtagatcgg ctttccgagt tcacgctgcg cggcctcgtg
     9301 cgcggcatcg cggtcgatat ctatggtcgg cacgtcaggc ttggcgggtc agccagaggt
     9361 ggtcggtgga atcggccgga gcgccgggcc cgaacgccgc tccggtctgc agcacgaggt
     9421 cgaacgcctc gccgcggatc cggcggtcgg tgtacagcag cacgacgacc ccggacgaga
     9481 aaggcgcggt gatgatttga ctgatcgcgc tgccgatcga catcaccacc agcgaggcga
     9541 gtgtcgccag cgtggatgac gctgagatga gcaggatttg gccaccgaag ctgaatggaa
     9601 cggacaccgc tccggcaacc accgcggcca cgattccgcc gagcaaccag ataccgaaca
     9661 cgcgccagaa atcgttgcgc accaatttgt acgagcgtga catcgcgggg atgatggcga
     9721 gtcgctccag cacgatgacc gacggcgtga agctgagcac ggtgaccaaa tagacgagac
     9781 cggcgaacat ggccagcacc aacggcaccg cgatgacgac ggctgccaca ccgcccgccg
     9841 ccgcgccgac gccggcgatg atcagcaaca ccaccgcgat gattaccgcc acgccgatgc
     9901 cttccagcac cgtgaagccg attaacgccc acagcctggg acggagccgt cgccacgcct
     9961 caccgatggt gatgtgcgcg ccaaagacgg cgcgtccgat cacgacagtg agcatgccac
    10021 tcaacaggat cgacgacagc cacgtggcga tggcgccggc gaagctcgat gccgacgaac
    10081 tgagcagcac acccgtcgac acctcctcgc cggccaatgt cggctggagc ccaccggtga
    10141 tggcgagtgg gcccaccgac aggatgagtg cgagtagctg cgcggccacc accacgacgg
    10201 tcgtcagccc cagtgtcgct ttgggattcg cgcggatgta ggcgacggcg ccgttgaaga
    10261 tgtccgacag ggtcaacggc cgcaacggaa tgacgcccgg cttgaagggc tgcacccccg
    10321 gcggcggacc gtatccgggt gggaaaccgt atccgggtgg cggatatccg ggtggcggat
    10381 aaccgggcgg cggatagcca ccgaaagggc cgtagcccgg cggcgggccg ccgtatcccg
    10441 gtgggggcgc atagcccggc gggggatagc cgggtggcgg atagccgcca ggcggcggat
    10501 agccctggta agcggcttgc ggatcatcct gatagcccgg tgatgggtag ccggggggtg
    10561 gatagcccgg cggcggcgga cctgccggct gaaattcgcc ggcgtcgttg ctcatgttca
    10621 ccatcctgtc gatgactgtg cgaattgaca acgtcgaccg gcgtagcgtt tgcgtcatgg
    10681 cggaactcaa agaccggctg cgtgccgacc tgaccactgc gatgaagtcg caggacaagt
    10741 tgcgcaccgc aacactgcgg atggttctgg cggcgatcca gaccgaagag gtcgccggca
    10801 aggcgtcgcg tgagctctcc gactccgagg tgatcaaggt gttgtcccgc gaggcgaaga
    10861 agcgcagcga gtcggcagag atctacacgc agaacgggcg cggtgagttg gccgccaacg
    10921 agcacgccga gggccggatc atcgacgagt acctaccgac tccgctcacc gaggcggaac
    10981 tcgccgatgt cgccgacacc gcgatcgcgc aagtcgccga ggacatcggg gagcggccca
    11041 gcatgcggca gatgggtcag gtgatgaagg ccgcgaccgc aatcgccgcg ggcaaggccg
    11101 acggcgcacg cctttctgcc gcggtcaagg ccaggctgta gccgtttcgg tatcaagcac
    11161 ccgggtaccc gagtgccatg acgcgttttg gctacaccct gatgacagaa cagagcggac
    11221 ctaaagacct tgtccgttat gccgtttcgg cggagcgcgc aggattcgac ttcgaagtct
    11281 cctctgacca ctatttcccg tggctttcgt cgcagggcca tgcgccgtat gcgtggtcgg
    11341 tgctcggcgc ggtcgcccac gcaaccgaac aggttgagct gttcacctac gtcacgtgcc
    11401 cgaccatgcg gtaccacccc gccgtcgtcg cgcagaaggc cgccacgctc cagatcctcg
    11461 ccgacggccg gttcacccta ggcgtcggca gtggtgagaa cctcaacgaa catgtcgtcg
    11521 gcggacgctg gccgacggtc tcgcgacgcc agcagatgct caaggaagcc attcaaatca
    11581 tccgcgagct gtttaccggc gaactggtcg actggaaggg cgagtactac gaggttgatt
    11641 ccgctcggat ctgggacgtg ccggagactc ccgtcgccat tgccaccgcg gtgtccggtg
    11701 agcgctcggt cgacatgttc gcgccgctgt ccgaccatct catcgcggtc gagccgaaca
    11761 aagagatcgt cgacgcgtgg cagaacgcac gacgcgcgac tgggctgccc ggcgaggtga
    11821 gggtgatcgg acagattccg atctgttggg atcccgaccg tgacgcggcc gtgcagcgcg
    11881 cacacgatca gttccgttgg ttcggcggcg gctgggctgt caacgctgac ttgccgacca
    11941 cggcgggctt cgagggtgcc acgcagttcg tccggccgga ggacgtcgcc gagtccatcc
    12001 cgtgcggacc ggatctcgac gcgatcgtcg aggcggtgag caagtactgg gaggccggct
    12061 tcaccgacat cgcgctggtg cagatcggcg acgacagcca ggagctgttc ctcaaggaag
    12121 cggcgggctc gctgctggag aagctccgca ccgaatccga ctgagaggaa ccgactatgc
    12181 cgcgcggaaa agggatctac gacgacgaag acgccgacga gccgaagggc ggacgtccag
    12241 ggccggcaga tgaaggccgc gacggcggta tggcgacgcg ggagaacgca gccgacgttg
    12301 cagaaactgg gggagagccg accgcttgag cgttagtcgc tctcgcgccc ttcgcggctg
    12361 cgccgatagg cggcgcgccg gaagtctgcc agccagccgg ggaccagccg cacatcttcg
    12421 tcactgtgca agacggggtc gaatgcgatg tcgtcgttgc tggggtcgag gtggcggagg
    12481 ctaagccgcg cgagcggcga gaagccgccg gtgcccttgg cctgatcgat gtcgaaatca
    12541 attgcgcccg aatctatttg atttcggatc gtgtcgagag acaagcccgg ctcgtcgatc
    12601 ttggtgacca atctggcacg tacccaccat gcgtcgccgt ggaagcgcaa cggcatcagg
    12661 caggagaacg atgttttcga ccaggacctg gttggcgcga gaacgatgcg gctgttggcg
    12721 atggtcgacg ccagcagcac atcccagggc acgcacgatc gcagatcctg cggaggagga
    12781 attcgccatg ccagcccggc gatgtcgggc aacgcgcgag gtaggccgat ccctttcgag
    12841 acgcgaccga tcacgtcaca ggactgcatg ggcagcccct cgttcggcgg cgcaatccgt
    12901 tcgagaatgc cctcggcgag cacgccatca ggatggaaca cccgagtccg gcggacggca
    12961 gcgctgagcc tgattggcaa cgccacgatg tcagaagctt gcacggctta ggtgtgcccg
    13021 ccacgccgac ttacgaaacc actacctcgg gtttaacaac ggcgatacag ggcactagag
    13081 acatcaacgc cgacatctcc tgatgcccaa gcaaacactg tcgaaaggcc gatgttgact
    13141 accgcataca ccgacctgga tatcccagcc gtcaccgaag gcgtctacgt cccgcaggaa
    13201 gacacgcaac tgctcattga catcatggac aagacggggc tctcccgtgg ccaccgggtc
    13261 gccgacctgt gtaccggtag cggtgtggtg gcgatcgctg cgtacgagca gggcgccgcg
    13321 gaggtcaccg ctttcgacat ctgcccgcgc gccgtacatt gtgcgcgcgc caactccgct
    13381 gccgccggcg ttgacgtggc tgtacacctc ggatcgtggg cgcgcagcac ggaattcggg
    13441 ccgtacgact tggttgtctg caatccgccc tacgtgccgc gtgatccgaa cgacgaggaa
    13501 attatgctgc cgtcgcggct cggacccgcg tgggcgtggg atgccggcta cgacgggcga
    13561 ctggtgctcg acccgctgtg tgctgccgcg tccgacctac tcgctgacgg aggcacgatg
    13621 ctgctcgtgc agtccgagtt cgccgcgcca cgtcagacgc ttgtggcgct ggccagctcc
    13681 ggcttggaca ccgaaatcgt tgcctggcaa tggatcccat tcggtcccgt gttgtcatcg
    13741 cgggcagagt ggcttgagga cacgggtcgg ctcgagccgg gccgccgtga ggaagaactg
    13801 ttggtgatcc gggcggacaa gccatgaggg atgacaaacc acgcatggtc cgggtggtgc
    13861 cgtccggccc ggtgatggtg gaaggcccgg tacgcatcga gatgcccgac ggcaccgtgg
    13921 tggaatccga taggttcatg gtcgcgattt gcacctgccg ccgcagcaag agctatcccc
    13981 tgtgcgacac cagccaccgg tgccgtcagc ggcccgagcg atcaggccag cggccggcgt
    14041 agcgatgatc ggcccgcccg ccaacaattc atgacatgcg cggcgagccg atattcgacg
    14101 acgtcgcgcg ccttgatacc gaataccacg tcgcggtcca gatgtggttc gcgcgcaacg
    14161 agatccccga cgacgtcggt gcgcaccact tgttcgtgca ccgcgtcggc ttcgatgtgt
    14221 tcgcggtaga aaccgatgca cgcttctggc gcgcccatgc gctcgagtgc atcgacgaga
    14281 cgccgtgagc ccggcgacga cgtgatctcc gtcgacgcga aatgaccgat cgcggcaccg
    14341 cgcagctcgc ggtgcagacc gaacaatgac atgaggttga ccgatgccag cgcgtcggcc
    14401 ggcacggcgt cgagatacgc gaggtaggtc gagtcgaggc ccgcggcgtc caacaagtcg
    14461 gcgaagagtt gttggtgcac caaagcccca cggccgccgc cgtattcgtc gaactcgacg
    14521 gcgacgaacg atgctttgga ctgcccgatc aacctcggta tcgcgaaggc gtgcggatcg
    14581 gcttccttga ggtgatacag cgagcgctgc acgaagtact cccgcatctg ctcccacgtg
    14641 ccggtgtcgc gcaggtaata cgacgggccc gtaccgtcga cgggttccat cgacaacgcg
    14701 tccatcgcgt cggcggcggt ctcgtcgcgg gagatctcac cgacgtcgtt gcgcaccgcg
    14761 ctcaggaacg tctcttcgag gcggccgcgc acatgcagta gccccggatt ccactcccag
    14821 cgtgggttga ctccctcgaa tccgcggtag tgcaactcgt agcagacgta aagggccagc
    14881 tgcagatcga ggccgtacgg gtcggcctca caaagcggca cgtcgatcgg ccgggcatgc
    14941 agcgcaggcc ttccgcgcgc cagggtgtcg atcacggccg acgaaagtgg accgacggcg
    15001 cgggggagcg tgggttcgac cgaggtcgac ggcaaagtca cgcggcaggt aattccccag
    15061 acccgggagg gtcaaaccac cgtcggagcg cacgcctcga tgagctcctg cggattggtc
    15121 acgctgacgt agagcgtgcg caacttgatc ggcacgccca ttacgtaggc ccgcgcgtag
    15181 accctgtcgt tgttcggctt cgcatccttg attgacgcca gcgcaatttc cgtacggaat
    15241 ctccagccga agcggacgtg cagtttgtcg tcttcgacgc ctatcgcact gtgcttgggc
    15301 cccagaccaa atggtacgga gaagggcaga aaccagcggt cgtaccgcaa atccgtagtc
    15361 atgattgcga gcctagtgtc gtgcaggttc gtttagtcct agagttggta gggaagtcga
    15421 cttccagcgt tgtcgagtcg aggggactca atggtcggca cactgctcgt tatctatgtc
    15481 atcggtctgc tgccggtgag ctgcgcggcc ttcgcggcca gcaagcggtt cgccgatcgc
    15541 cgggcacccc cggatctgcc gacggcggtg atgctgagct tggtcgccgg tgcagtctgg
    15601 ccgctgctgg tcgttgggct gatagagctc agctcggtca tgatgctctt caaggccccg
    15661 tcgaaggcca gcggcggcgt cggcatattt gcctagatgg cgataagcct cttgtcgggg
    15721 tactcggcgc tgcgcgccta cgaccccttt catcgcctcg ggcgaacgag caagctcgct
    15781 cgcctctcgg ctcagtcggg gtggcgggat tcgaacccac gacctcttcg tcccgaacga
    15841 agcgcgctac caagctgcgc cacaccccgc gtgaagccac gacagcgtat cgcaccggct
    15901 gccggtgaag ccaaacgcat atcgggccca cgccgggtga gcgcggtcac tagcggcggg
    15961 tttccctcag caccgccaat cgttcgcggt attcggcctc gttgacctcg cccttggcat
    16021 aggcatctcg tagcgtgcgg atgccatggt tgccgcggta ggcccgcacg atcagccaga
    16081 cggcggtgat caccagcgcc aggaatacca gccgagcgac gaggaaccat gggtgccagc
    16141 cgtagtggcc ccaccgttga ccttcctctg ccaggaaagt ggttgctgtt ggagcgattt
    16201 cgtagatgct catgccatcg agtctgcgcc ccgccgggtg tcattgcatc ggcctgcagc
    16261 cgtcaatctc gatacgcccg cacgcgtatc agccgcgcca tccgggacgc accagcccgc
    16321 tttcgtaggc catcaccacc aactgggtgc ggtcccgcgc gcccagcttg gtcaggattc
    16381 ggctgacgtg cgtgcgggcc gtggcggggc tcatgaacag ccgctcgccg atctcggcgt
    16441 tcgtcaagcc ctcggcgatc agcgccatca cttcgcgttc tcgcgaggtc agctcggcta
    16501 gcgcggtcga cgcgcccgac ggtggtttgg tgcgagcggc gaactccgct accagcctgc
    16561 gggtcacgga cggcgacagc agggcctctc cgtcggcgac aacccgaacc gcccgcacca
    16621 gctcggcagg ctcggtgtgc ttcaccagga acccgctcgc tccggcgcgc atcgcctcga
    16681 acacgtactc gtcgagctcg aacgtcgtca gcaccaccac ccggaccgtc gccagggcgg
    16741 gatcggcggc tatcgcccgg gtggcggcca gcccatccat ctcgggcatc cggatatcca
    16801 tcagcaccac atccggacgt agctcgcggg cggccgccaa cgccgcggtc ccagtgtcgg
    16861 cctcggcaac cacggtgatg ccgtcctgcg cgttgagcaa tgcgacgaag ccggcccgca
    16921 ccagcgcctg gtcatcggcg acaacaacgc gaatcgtcat gattcacagt cactttcgac
    16981 gggcagtgtg gcccgcacac tgaacccacc gccaggatgc cgtcgcaccg agagatctcc
    17041 gcccagagcc ctcgctcgct cccgcatccc agtgatgccg ctgccacccg acgacgggcc
    17101 ggcattgaga ggtcgcccgt cgttgccgac ggcgaccgct agctcgtcgg cgccgtaagt
    17161 gactgtcact tcggcgtttt ccgccgtcga gtgtcgcacc acattggtca gtgactcctg
    17221 cacgatgcga gcggcggcga cgtcgatcac cgtcggcagc ggtcgtggct caccctgcac
    17281 ggctgttgtc acggtcaacc ccgtcgcgcg ggcggtgccg accaggcggt ccaaatcggc
    17341 gattcctggc gttggcgcgg tgggttgacc agggtcggca cgcaacgccg tcaccaatga
    17401 gtgcacatcc gaaattgcct gtctgctggc atctttgatg gctgccagcg ccggcccggc
    17461 ccgctccggt tgatcgtcga gcagctccag cgcgaccgag gactgcacat tgatcatcga
    17521 aagactgtgc gcgagtacgt cgtgcaactc gcgggcgatg gacagccggg cctgagtggc
    17581 ccgccgctcc tgttccagct gcgtctcgcg agccatcgcg gccctgcgct gacgcctcgc
    17641 gtcgagcacc gcacggcgct g
//
LOCUS       DASRJA010000225         6546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_630819,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000225 DASRJA010000000
VERSION     DASRJA010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630819"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFC01_10110"
     CDS             <1..759
                     /locus_tag="VFC01_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80024.1"
                     /translation="RSLDSVSYFFRGLWGSGPTDIWAVGFRSFNNTTPVIFHYDGNGW
                     ARDASVADTLPWPPLSDVWGTSASNVWAVGYGGWISHFDGSRWTHASVGPAFYSDRIW
                     GTSATDAWTVGPQTVLHHDGIGWYSQSDSVLTARQLSAGWGARQDDQWAAGSTERGFL
                     HFDGSRWSPVVPDDATSGFIYGMWGVTPSRIWAVSRTGTTGPGVGAIWEYNGTQWLIV
                     ARDTINLTGVWASSASDAWVVGDFGTVLHGTLAH"
     gene            1600..5691
                     /locus_tag="VFC01_10115"
     CDS             1600..5691
                     /locus_tag="VFC01_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HZN80025.1"
                     /translation="MTLSAPKAGHMVSLVFARHSPRSDASMVRNTLRTGLHRVLAVMT
                     LCVLITCTDEQSPLAPPDAGIPNFAAGASFSGPVVFVGAGDIAACNNDNDEATAQLID
                     GIPGTVFTSGDNAYPFGRTIDYQNCYEPTWGRHKARTWAAMGNHEYDSSATADPSFDY
                     FGDRVGPRGLGYYSFDLGAWHIVVLNDNGQFVPFTANSTQDQWFQADLAAHPNLCTLV
                     MWHQPYLFSNKNPSGSITSPARKIFWDRAYAAGVDVVVNGHQHQYERFLPMTPDLVVD
                     TDRGIHAFTIGTGGESTAQPVLIAPNSVVQAGTFGVMKFTLKDGGYDWQFLPIPGQTF
                     TDAGSASCHDAPGGVSATQTTVTASPTTILKGTETSTITVTAKDANGNPVSGAFVSLS
                     ATGTGNTLTQPAGYTDVNGVATGTLSSTVTEIKTISAAVSGIAVAQTASVNVTAAVSA
                     SKSKVATAERYIAVGTGTSTITVTALDASGRPVSGATVTLQATGSGNTLTQPTALTDA
                     SGVTTGTLSSTVAGTKTVSATANGTLISQTASVAVTMPSSSRSTLSTSPLSFTAGTGS
                     STISVTVKDGGGHALAGAAVLLSATGTGNVVTQPAALTDSKGVAKGTLASTDAGAKVV
                     SATANGVLITKTATVTVNANPVSASLSTVAAAPTSVPTGATSTITVTVKNNSGQPVSG
                     ATVVLAATGSGNALTQPAGVTNASGVATGSLSSTVAETKTVSATANGIALTQTASVTV
                     TSGGVSASQSTLGASPTSISAGSGTSTITVTAKDAGGNPISGATVVLAASGTGNTLVQ
                     PATVTDPSGVATGTLSSSVVEAKTVSATVNGVSLTQTATVTVTAGSVSAGQSTLGASP
                     STIQAGNGTSTITVTARDAGGNPISGATVLLAATGTGNTLVQPAGVTNASGVATGSLS
                     SSVAETKTVSASISGTAITQTASVTVTPVTISPTLSSVGATPASFTAGSGSTTITVTV
                     LNGNGQPVSGANVVLSSTGTQNTLLQPSGPTDVNGVATGTLSSNKAELKTISATADGV
                     AISQTAAVTVTAGSVSAATSVVTAAPTSIAPGTETSTIRVTAKDGFGNPVSGATVTLA
                     ATGSGNTLTQPATTTNSIGVTSGTLSSTVAEPKTVSATANGVAIVQTATVTVTAPSGG
                     AITHTLLTSGNDVNNVKIYTTASIAPASNALVTVAVATHNSTSAAPVPTLTGGGMASW
                     EVVATITYDTVGLPRKRVTIFRAMSPSPGSGPITITSSATLSNCQWIVSQWSGVETGG
                     TNGSGAIVQTGTNAADTVTTLATSLAVFGNGANVAYGVFGVNSNVVAITPGTGFTEID
                     EQPSTESTAADLFAEWAVNRPWINAIWAKKNAAALGVEIKAAP"
     gene            5752..6204
                     /locus_tag="VFC01_10120"
     CDS             5752..6204
                     /locus_tag="VFC01_10120"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003938593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN80026.1"
                     /translation="MRPNVEPQDPDFRARVKDSFSHQAFMATIGASLQLVEPGEVHIA
                     LPFSTQLSQQHGYLHAGATAAIADSANGYAALTLAPAGADVLAVEFKINLMAPAAAPR
                     FLARGRVLRPGRRLSVCLAEVVALNDASQEVIATMLSTISIRAGGQPA"
     gene            6357..>6546
                     /locus_tag="VFC01_10125"
     CDS             6357..>6546
                     /locus_tag="VFC01_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80027.1"
                     /translation="MTAAGVFGKLQLRDETEIVVLNAPPSFESALKQLRGVTIHRTVS
                     RSPIRFALAFVARQAELAR"
BASE COUNT         1189 a         2203 c         2094 g         1060 t
ORIGIN      
        1 cgctcgctcg acagtgtgtc gtacttcttc cgtgggttgt ggggcagcgg tccgacggat
       61 atctgggccg tcggattccg cagcttcaac aacacgacac ccgtgatctt ccactacgac
      121 ggaaacgggt gggcacggga cgcgtcagtt gccgacactc ttccgtggcc accgctgagc
      181 gatgtctggg gaacctccgc gtcgaacgtc tgggccgtcg gttacggcgg atggatctcg
      241 cacttcgacg gttcgcgctg gacccatgcc tcggtcggtc cggccttcta ctcggatcgg
      301 atctggggaa cgtcagcgac ggatgcctgg acggtaggtc ctcagaccgt tctgcaccac
      361 gacggaatcg ggtggtactc gcagtcggac tcggtgctga cggcgagaca actctcggcc
      421 gggtggggag cacgccaaga cgatcagtgg gccgccggct ctacggaaag ggggttcctg
      481 cacttcgacg ggtcacgctg gagccccgtc gtccctgatg atgccacatc cgggttcatc
      541 tacggcatgt ggggggtgac gccgtctcgc atctgggcgg tctcgcggac gggcaccact
      601 ggtcccggcg ttggggcgat ctgggagtac aacggcacgc aatggctcat cgtggcgcgg
      661 gacacgatca accttactgg cgtgtgggcg agctccgcgt ctgatgcttg ggtggtcggg
      721 gatttcggca cggttcttca cggaacgctt gcacactgac gcgggcccac gctagcgctt
      781 cgcggcctga tcgccacgag ccgagtgcgc gtcgcacgcg cacccgaggg gggaggcacc
      841 aatgcgccgc cagttggtac tgatggtgtg cggcacgctc ctgtccaggc tgatggccgc
      901 gcagcaggtc gccagtccta gctctttctg ctggcgcgcc cggccagtgg agtactgtcg
      961 ggcgacgatc ctcacgaacg ctggcggtta cctggtgagc gggccagcct ccggccccgg
     1021 ggccgttgcc gactggggtg tactgatcaa caccggcgct cgaaccgccc tcggagcatc
     1081 ggtattcgcc tcacagagca gatatggatt cgagctcggc ccagcggtgc attatcgtcg
     1141 ctggcttggc ccggaccaga gcatcgacct cgccttgggg atacctctgt tcagttccga
     1201 aggctccgtg gccgttgcgc cctacggcct cgtcaagtac aacccggtcc actgggccgg
     1261 actcgccgtg cggccggagt ggcggcgcgg ctcctacaat gggcaaccgt actccttcgt
     1321 gatttcagtc ggagcggagt tcggttgggt cccggggtcc gtcatgaccg tggccggtgg
     1381 agttgcggct ctcgctttgc tatcgtccac ttccaactcg aactaattgg tgtcggcaac
     1441 gcgtgcagcc tcgaaaagag ggcgcaatgt cacaggttag cggttcttcg tagtccctta
     1501 tcctctcgcc gcagcatttc tgccatctcc tcgccgcagg tggatgacga gccataagcc
     1561 gtaacgagta acgctgcgcg caaaccttag gcacagaact tgaccctgtc cgcccccaag
     1621 gcggggcaca tggtctcgct ggtctttgcc cgccactccc cgaggagcga cgcatcgatg
     1681 gtccgcaaca ccctccgcac cggactccat cgagtcctgg cggtcatgac tctgtgtgtg
     1741 ctcatcacct gcaccgatga gcagtctccg ctcgctccgc cggatgcggg gatcccgaat
     1801 ttcgcggctg gcgcgtcatt cagcggaccc gtggtcttcg tcggggccgg cgacatcgcc
     1861 gcctgtaaca acgacaacga cgaagccacc gcgcagctga tcgacggcat cccgggtacc
     1921 gtattcacgt ccggcgacaa cgcgtatccc ttcggccgga cgatcgacta tcaaaactgc
     1981 tatgaaccca cctggggccg tcacaaggcg cgcacctggg cggccatggg taaccacgag
     2041 tacgactcgt cggctaccgc tgacccctcc tttgactatt tcggggaccg ggtcggcccc
     2101 cgcggcctgg gttactacag cttcgatctc ggggcgtggc acatcgtggt gttgaacgac
     2161 aacggccagt tcgtgccgtt cacggcgaat tcgacgcagg accagtggtt ccaggccgac
     2221 ctcgccgcgc atcccaatct ttgcacgctc gtcatgtggc accagccgta tctcttctcg
     2281 aacaagaatc cctccggctc catcaccagc cccgcacgca agatcttctg ggaccgcgcg
     2341 tacgcggcgg gcgtcgacgt ggtggtcaac ggccaccaac accagtacga gcggttcctg
     2401 cccatgaccc ccgacctggt cgtggacacc gaccgcggca tccacgcgtt caccatcgga
     2461 acgggtggcg agagcaccgc gcagcccgtc ctcatcgccc cgaacagcgt cgtgcaagcc
     2521 gggacctttg gcgtgatgaa gttcacgctg aaggacggcg gctacgactg gcagttcctc
     2581 ccgattcccg gccagacgtt caccgacgcc ggtagcgcct cctgccacga cgctcccggc
     2641 ggcgtgtcgg ccacccagac gaccgtcacc gcgtcgccga ccaccatcct gaagggcacc
     2701 gagacttcca ccatcaccgt cacggcgaag gatgcgaacg gcaacccggt gagcggagcc
     2761 ttcgtgtcgc tctcggcgac gggaaccggc aacacgctga cccagcccgc cggttacacc
     2821 gacgtgaacg gcgtggcaac cggaacgctg agctcgaccg tgacggagat caaaaccatc
     2881 tccgcggcgg taagcggcat cgcagtcgca caaacggcct cagtcaacgt gacggcggcc
     2941 gtttccgcgt cgaaatcgaa ggtcgcgacc gcagagcggt acatcgccgt gggaacgggg
     3001 acctcgacca tcacggtcac ggcgctggac gcctccgggc gccccgtgag cggggccacc
     3061 gtcacactgc aggcgacggg aagtgggaac acgctgaccc agccgacggc cctgaccgac
     3121 gcgagcggtg tgacaaccgg tacgctcagc tcgacggttg ccgggacgaa gaccgtctcg
     3181 gccacggcca acggcacgct gatcagccag accgcgagcg tggccgtcac gatgccgtct
     3241 tcgtcaaggt cgacgctctc gacctccccg ctgtcgttca ccgcgggaac cggcagctcc
     3301 accatcagcg tcacggtgaa ggacggaggc ggtcacgcgc tagccggggc cgcggtcctg
     3361 ttgtcggcga ccggcacggg caacgtcgtg acacaaccgg ccgcgcttac ggattccaag
     3421 ggagttgcga agggcaccct ggcgtcgacc gacgcggggg caaaagtcgt ctccgcgacc
     3481 gcgaacggcg tcttgatcac caagaccgcc accgtgacgg ttaacgcgaa cccggtgtcc
     3541 gcctcgttgt cgacggtcgc ggctgcgccc acttcggtcc cgaccggcgc gacgtcgacg
     3601 atcacggtca cggtgaagaa caacagcggt caaccggtca gcggcgccac tgtcgtgctc
     3661 gccgccaccg ggagcgggaa tgcgctcacg caacccgccg gcgtaacaaa tgcgagcggt
     3721 gtcgcgaccg ggagcctcag ctcgaccgtc gccgaaacca aaacggtttc ggcaacggcg
     3781 aacggcatcg cgctcacaca aaccgcgtcg gtgacggtga cgagtggagg cgtgtccgcg
     3841 tctcaatcga cgctgggcgc ttcgccaaca tccatctcgg cgggaagcgg cacgtccacg
     3901 attacggtca ccgccaagga cgccggcggg aatcccatca gcggcgcgac ggtcgtgctc
     3961 gcggcgagcg ggaccggcaa tacactcgtc cagccggcaa cagtgaccga cccgagcggc
     4021 gtggcgaccg ggacgctcag ctcgagcgtg gtcgaggcga agaccgtctc ggcgacggtg
     4081 aatggcgtct cgttgacaca gactgcgacc gtgacggtca cagcgggaag cgtatcggcc
     4141 ggccaatcga cgttgggcgc ttcaccctcg acgattcaag ccgggaacgg cacgtccacg
     4201 atcacggtga cagccaggga tgccggcggc aacccgatca gcggcgcaac ggtgctgctc
     4261 gcagcaaccg gaacagggaa cacgctcgtt cagccggccg gtgtgaccaa cgcgagcggc
     4321 gtcgccacgg ggagcctcag ctcgtcggtg gcggaaacga aaacggtttc ggcgtcgatc
     4381 agcggaaccg ccatcaccca gaccgccagt gtcacggtca cgccggtgac catctcccca
     4441 accttatcga gtgtcggcgc caccccagca tcattcaccg cgggtagtgg gagtacgacc
     4501 atcaccgtga ccgtcctgaa cggcaacggc cagccggtca gcggtgcgaa cgtcgtgcta
     4561 tcgtccacgg gcacgcagaa cacgctgctc cagccctcgg gtccgaccga tgtcaacggc
     4621 gtggcgaccg gaacgctgtc ctcgaacaag gcggagctga agacgatctc ggccaccgca
     4681 gacggcgtgg cgatctcgca gaccgcggcc gtgactgtaa cggccgggtc ggtctcggca
     4741 gcgacctcgg tggtcacggc tgcgcccacg agcattgctc cggggacgga aacttcgacc
     4801 atccgggtca ccgcgaaaga cgggttcgga aaccccgtca gcggcgccac cgtgacgctc
     4861 gccgccacgg gcagcggcaa cacgctgacg cagcccgcca ccacgaccaa ttcgatcggg
     4921 gtgacgtccg ggaccctcag ctccacggtc gccgaaccca aaaccgtttc ggctaccgcc
     4981 aacggcgtcg cgatcgtgca gacagcgacg gtcacggtga cggcgccgag cgggggggcc
     5041 atcacgcaca cgctgctcac ctcgggtaac gacgtgaaca acgtgaagat ctacacgacg
     5101 gcgtcgatcg ccccggcgtc gaacgctctc gtgaccgtgg ccgtggccac gcacaattcc
     5161 accagcgccg caccggtgcc gaccctgact ggtggcggga tggccagctg ggaagtggtc
     5221 gcgactatca cctacgacac ggtcggcctg cctcgcaagc gcgtaacgat cttccgcgcc
     5281 atgagcccgt cgccaggcag cggtccgatc accatcacgt cgagcgccac gctctcgaac
     5341 tgtcaatgga tcgtgtcgca atggagcggc gtcgaaaccg gcggcacgaa cgggtccggc
     5401 gccatcgtcc aaaccggcac gaacgccgcc gacacggtga cgaccctcgc tacgtcgctc
     5461 gcagtgttcg ggaacggggc gaacgtggcc tacggcgtgt tcggcgttaa cagcaatgtg
     5521 gtcgcgatca cgcccggcac gggattcacc gaaatcgacg agcagccgtc gaccgagagc
     5581 acggccgccg acctgttcgc cgaatgggcg gtcaatcggc cctggatcaa cgcgatctgg
     5641 gcgaagaaga acgcggcggc cttgggggtg gagatcaaag cggcgccctg aacgtaccag
     5701 cgcatgacgc ggtcgtggcg cgacggcgct cgcaagggca gcttatcgct catgcgtccc
     5761 aatgttgagc cacaggatcc cgacttccgt gcgcgggtca aggacagctt ctcacatcaa
     5821 gcattcatgg caacgatcgg cgcttccttg cagctcgttg aaccggggga agtgcacatc
     5881 gcactgccgt tctcaacgca gctgtcgcag caacacggct acttgcatgc cggggcgaca
     5941 gccgctatcg ccgacagcgc caatggatat gccgcgctta cgttggcacc ggccggtgcc
     6001 gatgtgttgg cggtggagtt caagatcaac ctgatggcgc cggcagcggc gccccgattc
     6061 ctcgcccgcg gcagagtcct caggccaggt cgtcgccttt ctgtttgcct tgccgaagtc
     6121 gtcgccttaa acgatgcgag tcaggaagta atcgcgacca tgctctcgac gatcagcatc
     6181 agagcggggg gtcagccggc ctgacacgcg gcagccggcc ggaaacgacg cttcggtatc
     6241 cagatccagc cgacgggccc gacattgtcg aggtctacga gcgcgcttgc gcgggctgtc
     6301 cagagcgcat cacgttaggc ggcggaatgt gactaccagc caaccccgga gtaccaatga
     6361 ctgcagccgg cgtattcggt aagctccagt tgagagatga gaccgagatt gtggtcctca
     6421 acgcaccgcc gagctttgag tccgcgttga agcagttgcg cggcgtcaca atccatcgga
     6481 cggtatctcg ctcgcccata cggttcgccc tcgcctttgt cgctcgacaa gctgaattag
     6541 cacgcg
//
LOCUS       DASRJA010000226        13449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1195274,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000226 DASRJA010000000
VERSION     DASRJA010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13449
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195274"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFC01_10130"
     CDS             <1..760
                     /locus_tag="VFC01_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HZN80028.1"
                     /translation="AFQLLTGTPPFQHSNPAVVISQHLTADPPAIGTRRPELSSLGPV
                     FDKALAKSPNGRYDRCVDFARALAHHIGTENQQLSADATMLAHAATGPRHAKAQSKPR
                     RRVVLPVAIMLVVIAVVAIGFVLFDRMRDRTAEAKPQSPSAPSAPASPGNVTLPVVVV
                     GAKCATLGAAAITEGGTPAYCAHLPTTNATIWSLYPGEISSPTATAGPNDEVYPSETE
                     SPVLVCMEQTGQSRLDCHDDILQSNATATATPSP"
     gene            757..1323
                     /locus_tag="VFC01_10135"
     CDS             757..1323
                     /locus_tag="VFC01_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80029.1"
                     /translation="MKRLGFAALLFLVAACTGGQPVAENSPSTPTTEPIPSTRNIITE
                     GLHDKRVGQPGGFGCGEGPDAVCDVTFTVTAIEQDPPCPGGMLPAGKRLLRIEVDAQA
                     PLAYQYEPPPTALMLSHWALETADGAMHYDLQMVDACSPRPGVFAGRLAPATHPRDSV
                     VVIAPSQVTFLWLGYEKTAYRWPVRPPI"
     gene            1365..1973
                     /locus_tag="VFC01_10140"
     CDS             1365..1973
                     /locus_tag="VFC01_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZN80030.1"
                     /translation="MRKTTAGMFIALDGVVEAPDQWHFPYFNDEMGAAVGGQLGSADT
                     ILLGRGTYDSFAGAWPDREAGGGEDAEFAKKLGDTRKVVVSRQQLQFAWRNSEQLKGD
                     LAEGVAALKNEPGGDIGISGSVSVVRQLLAAGLLDELHLLVHPIVVGKGMRLFDEGQS
                     VPLKLLSSQTFETGVVYLVYAPAEAPAAAGYDEAKAHLPQND"
     gene            complement(1970..2872)
                     /locus_tag="VFC01_10145"
     CDS             complement(1970..2872)
                     /locus_tag="VFC01_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN80031.1"
                     /translation="MMRLGNAVLARVVEWQVAGLSFELFPQTPTEAWSELAADYTPMF
                     WSDAGWRIALQTWVIEVDGLTVLVDTGAGNHRDRPAMPPIDHLDTDFLDALRAAGVEP
                     DSVDVVVNTHIHSDHVGWNTMRDGDDWVPTFPNARYVMPEADYRHFHPDNADDWSAPR
                     TEADAARQAGWRIMFADSIVPVEEQMELWSEDHQLSESLRLRPAPGHTPGSSVLWLDA
                     GKPAVFVGDLTHCPIQMHRPMDPCAFDDDFAMAAVTRKRVLTEASRRRAAVIPAHYPG
                     RGGATVVARGDTFMVDDWLELPPI"
     gene            complement(2869..3267)
                     /locus_tag="VFC01_10150"
     CDS             complement(2869..3267)
                     /locus_tag="VFC01_10150"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN80032.1"
                     /translation="MALECINPADLPTPSTYTHVVVATGNRLVFIAGQEPEDVDGNLV
                     GAGDLAAQARQVFANLGRALAAAGARPEQVAKITIYVVGHRPEYLPVIEEGRVALFGD
                     HKPADTLLGVETLSQPDYLIEVDAVAVIDA"
     gene            complement(3277..6456)
                     /locus_tag="VFC01_10155"
     CDS             complement(3277..6456)
                     /locus_tag="VFC01_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052636 - arabinosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071766 - Actinobacterium-type cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase domain-containing protein"
                     /protein_id="HZN80033.1"
                     /translation="MSQDVRIARWTATIAGLIGFVLSVATPLLPVVQTTATLNWPQNG
                     QLNNVTAPLISQVPVTMTVTVPCGVIRAMPPAGGVVLSTAPKDGKQAALQSLFVNVNA
                     QRVDITDRNVVIASVPRARVAAPECQRIEITSSEAGTFATFVGLTDPTTGKEQRTGFP
                     DPNLRPAIVGIFTDLTGPAPPGLSASAVIDTRFSTKPTALKLAAMVLAIISTIVALVA
                     LWRLDRLDGHRMQRWIPQRWRTFSATDVVVVGGFLVWHVLGANSSDDGYILQMARVAG
                     HAGYMSNYFRWFGSPEDPFGWYYNLLALMTSVSDASIWIRLPDLVCGIVCWLLLSREV
                     LPRLGPAVAASKPAVWAAGLVLLAAWMPFNNGLRPEGQIATGALITYVLIERAIISGR
                     MTPAALAILTASFTLGIQPSGLIAVAALLAGGRPILRILVKRHRVVGTWPLVAPLLAA
                     GTVVLTVVFADQTLATVLEATRIRTAIGPSQAWYTENLRYYYLILPTVDGSLSRRFGF
                     LITALCLFTAMFVMLRRKRVPGVARGPVWRLMGVIFGTMFFLMFTPTKWVHHFGLFAA
                     VGAAMAAVATVLVSPAVMRWSRNRMAFLAAVFFTLALCFATTNGWWYVSSYGVPFNNA
                     TPKIVGITGSFLFFILFVITAIYAFWLHFAPRDHGEGRVARALTAAPIPLAAGFMVVV
                     FVGSMTAGIVRQYPTYSNAWANLRAFTGGCGLADDVLVEPDANDGFLTPLPGNYGPLG
                     PLGGVGPVGFAPSGVPEKIVAETIRMNLPMPGTDYDWDQPIKLKTPGVNGSTVPLPYG
                     LAPARVPLAGTYIEGPQQQSRLTSAWYQLPRPDDAHPLVVITAAGTITGDSVFNGRTD
                     GQKVELEYARPGPDGAPVPAGRLVPYDIGPIPSWRNLRFPRAQIPADAVAVRVVAEDL
                     SLTPGDWIAVTPPRVPELRSVQEYVGSDQPVLMDWAVGLAFPCQQPMLHANGVTQIPK
                     FRITPDYNAKRKDTDTWEDGLNGGLLGITDLLLRAHVMATYLSHDWGRDWGSLRKFDT
                     IVNAEAAQFEFGTATHSGLWKPGRIRIGP"
     gene            complement(6552..9806)
                     /locus_tag="VFC01_10160"
     CDS             complement(6552..9806)
                     /locus_tag="VFC01_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052636 - arabinosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071766 - Actinobacterium-type cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase domain-containing protein"
                     /protein_id="HZN80034.1"
                     /translation="MPAHRIARLIAVVAGIAGVLLCGLAPLLPVKQTTATIVWPQAPG
                     PNGLVSDVTAPLVSGAPQALDVSIPCRTIATLPAAGGVVLSTNPPGGIESSRNGLFVT
                     ANAANVVVAFRDSVAAVAPRPAVASGACSALHVWANPGAVGADFVGIPGATGTLSAEK
                     KPQVTGVFTDLQVAAQPGLSARIDVDTRFITSPTALKLGVMVLGVVCVVASIVALAVL
                     DRRSGRRVRDAWRRFWRVSLSTWIVDVGVIGTLLLWHLIGSTSSDDGYNLTIARVSAD
                     AGYAANYYRFFGATEAPFDWYQSVLAHMAAITTAGVWMRLPATAAAIGTWLIISRCVL
                     PRLGRRLANNRVAVWTAGAVFLAAWLPFNNGLRPEPLIAFGALAAWILVEDAIATRRL
                     WPAAVAIIVAVFSVTLAPQGLIAIAPLLVGARAVARIIPSRRPIDGLLAQLAPLAAAL
                     ALIFVVMFRDQTLATVAESARIKYKVGPTIPWYQDFLRYYFLTVEDSADSSLTRRFAV
                     LVLMLCLFGMLAVLLRRGGMPGMAKGPVWRLIGATAVGLLLLTFTPTKWAVQFGAFAG
                     LAGALGGVTAFAFARVGLHSRRNLAVYVTALLFVLAWATSGINGWFYVGNYGVPWFDK
                     QPVIVGIPVTGIFLALAIATGLLAGWLHFRMDYAGHTEVANTRRNRVLASTPLLVVAV
                     IMVVLEVGSMAKGAAQRYPIYTTAKANVDALTSGLSSSSCAMADDVLVEADTNAGLLQ
                     PVPGQRFGQYGPLGGESPVGFTPDGVSDTLEPAEPVTANPGTVNSPGSPNEPNIGIGY
                     AAGTGGGYGPVGINGSNVYLPFGLDPKRTPVMGSYNENTVAAKVTSAWYQLPPRAPDR
                     PLVTVAAAGAIWYYEEDGSFNYGQSLKLQWGVHRPDGSFQPLGQVQPIDIFPQKAWRN
                     LRFPLAWAPPEANVARIVADDPNLSTDQWFAFTPPRVPVLETAQQLLGSQTPVLMDIA
                     TAANFPCQRPFSEHLGVAELPEYRILPNFKQVVVSSKQWQSAEGGGPFLFIQSLLRTS
                     TVPTYLRDDWYRDWGWIERYDRVVPASVAPNAVIDQGSARVFGWSRSGPIRALP"
     gene            complement(9831..13070)
                     /locus_tag="VFC01_10165"
     CDS             complement(9831..13070)
                     /locus_tag="VFC01_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052636 - arabinosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071766 - Actinobacterium-type cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase domain-containing protein"
                     /protein_id="HZN80035.1"
                     /translation="MATDTAPQTGQLPSSPVTAAGNHRTARIIAIVAGLLGAALAIAT
                     PLLPVTQTTAQLNWPQNDVLQSVNAPLIGYVATDLNITIPCSAAAGLAGPENRGRTVL
                     LSTVPKQAPKAVDRGLLIERVNNDLLVIVRNTPVVVAPLNQVLSPACQRLTFTAHADK
                     VAGEFVGLVTGRDSDDPGKPLRGERGGYDFRPQIVGVFTDLSGPAPPGLQFSATIDSR
                     YSTSPTLLKMLAMVVGVALTGIALGALHVLDTADGRRVKRVLPRKWWSVTPLDGIVTV
                     VLVWWHFVGANTSDDGYILTMARVSEHAGYMANYYRWFGTPEAPFGWYYDLLALWAHV
                     STNSAWVRLPTLVMALACWWLISREVIPRLGHAVKTSRVAAWTAAGMFLAFWLPLNNG
                     LRPEPIIALGILLTWCSVERGVATNRMLPVAIAIVIGALTLFSGPTGIAAVGALLVAV
                     GPLKTIVARHTSRFGHLALLAPILAACTVTIILIFRDQTLVGEIQASTFKSAVGPSLS
                     WFDEHIRYERLFTTSPDGSVARRFAVLTLLLALAVSVAMSLRKGRIPGTALGPSRRIV
                     GITIISFLAMMFTPTKWTHHFGVFAGLAGSLGALAAVAITATAMKSRRNRALFAATVL
                     FVMALSFATVNGWWYVSNFGVPWSNQFPEWRFGFTTFLLGLSVLALLVAAWFHFSGRD
                     ESPPTRRNWQRMVQSPLAIAAWLLVFFEVLSLTLAMIDQYPGWSNGRSNLEALAGKTC
                     GLANDVMVEQDSNAGLLSPIGVPVGEALGSVTAEGFGPNGIPADVSADPVMESPGTFN
                     FADTETGTVSANEAGTEGGTTAAAGVNGSRARLPYSLDPARTPVMGSWRSGTQQPAVL
                     RSAWYQLPPRDKAGPLLVVSAAGRFDPGEVIVQWANDKGEPAGGVGFADVGSAPAWRN
                     LRAPMAAIPRDATRIRLVATDDDLNPQHWIAVTPPRIPELRTLQDVVGSQDPVLLDWL
                     VGMAFPCQRPFGHQYGVVEVPKWRILPDRFGAEANSPVMDNLGGGPLGITELLLRPTT
                     VPTYLKDDWFRDWGALQQLTPYYPNAQPARLDLGSATRSGLWSPAPLRH"
     gene            complement(13070..>13449)
                     /locus_tag="VFC01_10170"
     CDS             complement(13070..>13449)
                     /locus_tag="VFC01_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arabinofuranosyltransferase"
                     /protein_id="HZN80036.1"
                     /translation="ETVLLTADYSFLSYYPYYGFQGLTSHYANPLAQFDQRAAAVENW
                     ATLTTADQFIASLDKLPWKPPTVFLMRRGANDAYTLRLASDVYPNQPNVRRYHVALDK
                     ALFDDPRFDVSTVGPFVLAIRTG"
BASE COUNT         2356 a         4484 c         4563 g         2046 t
ORIGIN      
        1 ggccttccag ctgctgaccg gcacgccgcc cttccaacac tccaaccccg ctgtggtgat
       61 cagccagcac ctgacggcgg atccgcctgc gatcgggacc cgccgtcccg agttgtccag
      121 ccttggaccg gtattcgaca aagcgcttgc caagtcaccc aacgggcggt acgaccgatg
      181 cgttgatttc gcacgcgccc tcgcccatca cataggtacc gagaaccaac agctcagcgc
      241 tgacgcgacg atgttggcgc acgccgcgac gggcccgcgg cacgccaagg cgcagagcaa
      301 gccgcgacgg cgcgttgtcc tcccggtggc catcatgctc gtcgtgatcg ctgtcgttgc
      361 aatcggtttc gtgcttttcg accggatgcg cgaccgcacc gcagaagcca agccgcaatc
      421 gccatccgca ccgtccgctc ccgcgtcgcc gggcaatgtc acgttgccgg tggtggtcgt
      481 cggcgccaag tgcgcgacgt tgggtgcggc ggccatcacc gaaggcggca cgccggcgta
      541 ctgcgcgcac ctgcccacga cgaatgccac gatctggtcg ctgtatccgg gcgagatctc
      601 cagcccgact gccacggccg ggcccaacga cgaggtatat ccgtcggaga ccgaatcacc
      661 tgtgctggta tgcatggagc agaccgggca atcgcgcctg gactgccacg acgacattct
      721 gcagagcaac gcaacggcta ccgcgacgcc gtcgccatga aacgactcgg gttcgcggcc
      781 ctgctgttcc tggttgcagc gtgcacaggc ggacagcctg tcgccgagaa ctcgccgtcg
      841 acgccgacca cagagccgat tccgtccaca cggaacatca tcaccgaggg tctccacgac
      901 aagcgcgtgg gtcagccggg cgggttcggc tgtggggagg gcccggacgc agtgtgcgat
      961 gtcacgttca cggtcacggc gatcgagcag gatccgccat gtccgggcgg catgctgcca
     1021 gcgggaaaac gattgctgcg cattgaggtc gacgctcaag cgcccctggc gtaccagtac
     1081 gagccaccac cgacggcgct aatgctttcg cactgggccc tggagaccgc agacggcgca
     1141 atgcattacg accttcagat ggttgacgcc tgcagcccgc gtccaggagt gttcgcggga
     1201 cgactcgccc ccgctactca tccccgtgac agcgtggtgg tcatcgcgcc gagccaggtc
     1261 acgttcctgt ggctggggta cgaaaagacc gcgtatcgct ggcccgtgcg gccaccgatc
     1321 tgaacccgcg atgagtttgg cggccgccgg ttgtcagaaa ctccatgagg aaaaccacgg
     1381 caggcatgtt catcgcgctc gacggcgtcg tggaggcgcc agatcaatgg cacttcccgt
     1441 acttcaacga cgaaatgggc gccgcggtcg gcggccagct cgggtcggcc gacaccatac
     1501 tgctcggccg cgggacctac gacagcttcg ccggcgcgtg gccggaccgt gaagcaggcg
     1561 gcggcgagga cgcggagttc gccaagaagc ttggcgacac ccgcaaggtc gtggtgtcac
     1621 gccagcagct gcaattcgcg tggcggaact ccgagcagct caagggcgac ctcgccgagg
     1681 gtgttgcggc gctgaagaac gaaccgggcg gcgacatcgg gatcagcggt tcggtgtcgg
     1741 tggttcgcca actactggcc gccggtctac tcgacgagtt gcatctgctc gtgcacccga
     1801 tcgtggtcgg caagggcatg cgcctgttcg acgagggtca gtcggttccg ctaaagctct
     1861 tgtcgtctca gacgtttgag accggagtgg tgtacctcgt ctacgctccg gccgaggcgc
     1921 ccgctgcagc cggttacgac gaggccaagg cgcacctgcc gcagaatgac tagatcggtg
     1981 gcagttcgag ccagtcgtcg accatgaacg tgtcaccacg cgcgaccacc gtcgcaccgc
     2041 cacggccggg atagtgcgcc ggaatcaccg cggcgcggcg gcgggacgcc tcggtgagca
     2101 ctcgcttgcg ggtcaccgcg gccattgcga agtcgtcatc gaacgcgcac ggatccatag
     2161 ggcgatgcat ctggatcggg cagtgcgtga ggtcgccgac gaacaccgcg ggcttacccg
     2221 cgtccagcca cagcaccgac gaacccggcg tatgacccgg cgcaggtctc agccgaagcg
     2281 actcgctgag ctggtggtcc tccgaccaca gctccatctg ctcctcgacg ggaacgatgc
     2341 tgtctgcaaa catgattcgc caacccgctt gccgtgccgc gtcggcctcg gtgcgcggcg
     2401 cggaccagtc gtcggcgttg tccgggtgga agtgccgata gtcggcctcg ggcatcacat
     2461 aacgggcgtt gggaaaggtc ggcacccagt cgtcgccatc gcgcatcgtg ttccagccca
     2521 cgtgatcgga atggatgtgc gtgttgacga cgacgtcgac ggaatcgggc tcgacgcccg
     2581 cggcacgcag tgcgtcgagg aagtcggtgt ccaaatgatc gatcggcggc atcgccgggc
     2641 ggtcgcggtg gttgcccgcg cctgtgtcca ccagaactgt caggccgtcc acctcgatga
     2701 cccaggtctg cagtgcgatc cgccagcccg cgtcgctcca gaacatcggc gtgtagtcgg
     2761 cggccagctc actccacgcc tcggtgggcg tctgcggaaa caactcgaag gaaagcccgg
     2821 cgacctgcca ctcaaccacg cgggccagca ccgcgtttcc cagcctcatc atgcgtcgat
     2881 gacagcgacg gcgtctacct cgatcaggta gtcgggctgc gacaacgttt ccacgcccag
     2941 cagggtgtcg gcgggcttgt ggtctccgaa cagcgcgacg cggccctcct cgatgaccgg
     3001 caggtactcg ggccggtgtc ccacaacgta aatcgtgatc ttcgcgacct gttcgggcct
     3061 ggctcccgcg gccgcgagcg cccgaccgag atttgcgaag acctgccgag cctgagccgc
     3121 aaggtcaccg gcgccgacaa ggttgccgtc tacatcttcg ggttcctgtc cggcgatgaa
     3181 caccaatctg tttccggtcg cgacaacgac atgggtgtac gtcgatggtg tcggcagatc
     3241 ggcggggttg atgcactcga gcgccatgga tccaccctac ggaccgatgc ggatcctgcc
     3301 gggcttccac aacccgctgt gcgttgccgt gccgaattca aactgcgcgg cctcggcgtt
     3361 cacgatggtg tcgaacttgc gcagcgatcc ccagtcacgg ccccagtcgt gcgacaggta
     3421 cgtcgccatc acgtgtgccc gcaatagcag gtcggtgatg cccagcaggc cgccgttgag
     3481 gccgtcctcc caggtgtcgg tgtccttgcg cttggcgttg tagtcgggcg tgatccggaa
     3541 cttcggaatc tgcgtcacac cgttggcgtg cagcatcggc tgctggcacg ggaaggcaag
     3601 gcccacagcc cagtccatca gcactggctg atccgagccg acatattcct gcaccgaacg
     3661 cagctcgggc acgcgtggcg gcgtgaccgc gatccagtcg cccggcgtca aggacaggtc
     3721 ctcggcgacc acccgcaccg cgaccgcgtc ggcgggaatc tgcgcgcgcg ggaatcgcag
     3781 gttgcgccac gacggaatcg gcccgatgtc gtatggcacc agcctgcccg ctggcaccgg
     3841 cgcgccgtcc ggaccgggcc gcgcatattc cagttcgacc ttctgcccgt cggtgcgccc
     3901 gttgaacacg ctgtcgccgg tgatggtgcc cgcggcggtg atcaccacga gcggatgcgc
     3961 gtcgtcggga cgaggcagct gataccaggc cgaggtcaac cggctctgct gttgcgggcc
     4021 ctcaatgtac gtgcccgcca atggaactcg cgccggagca agaccgtacg gcagcggaac
     4081 cgtcgagccg ttgacaccgg gtgtcttgag cttgatgggt tgatcccagt cgtaatcggt
     4141 gccgggcatc ggcagattca tccggatcgt ctcggcgaca atcttttccg gcacaccgct
     4201 gggggcgaag cccaccggcc cgacaccgcc gagtgggccc agcgggccgt agttgccggg
     4261 cagcggcgtc aggaagccgt cgttggcgtc gggttcgacg agcacgtcgt cggccaggcc
     4321 gcagccgccg gtgaaggcgc gcagattggc ccacgcgttc gagtaggtcg ggtactggcg
     4381 cacgatgccg gcagtcatcg acccgacgaa caccaccacc atgaagcccg ccgcgagcgg
     4441 gatgggcgcg gcggtcaacg cacgcgccac ccggccttca ccgtggtcgc ggggcgcgaa
     4501 gtgcagccag aaagcgtaga tcgcggtgat cacgaacagg atgaaaaaga ggaaactgcc
     4561 ggtgattccg acgatcttcg gtgtggcgtt attgaacggc acgccataac tcgacacata
     4621 ccaccagccg ttggtggtgg cgaaacacaa cgccagggtg aagaacaccg cggccaggaa
     4681 ggccatccga ttgcgggacc atcgcatcac cgcaggggat accagcaccg tggccaccgc
     4741 cgccatcgcg gcgccgaccg ctgcgaaaag gccgaagtgg tgcacccact tggtgggcgt
     4801 gaacatcagg aagaacatgg tgccgaagat gacgcccatc aaccgccaca ccgggccgcg
     4861 cgcgaccccg ggaacacgct tgcgccgcaa catgacgaac atcgccgtga acaggcacag
     4921 cgcggtgatc agaaaaccga accggcgcga cagcgagccg tcgaccgtcg gcaggatcag
     4981 gtagtagtag cgcaggttct ccgtgtacca cgcctggctg ggcccgatcg cggtgcgaat
     5041 cctggtggcc tccaacaccg ttgccaatgt ctggtccgcg aacaccacgg tcagcacgac
     5101 ggtgcctgcc gccagcagtg gcgcgaccag cggccaggtg ccgacgacgc ggtgtcgctt
     5161 gaccaggatt cgcagaatcg gccggccgcc cgcgagcagc gccgcgacgg cgatcagacc
     5221 ggacggctgg atgcccaacg tgaatgaggc ggtcaggatg gccagtgccg cgggcgtcat
     5281 gcggcccgag atgatcgcgc gctcgatcag cacataggtg atcagcgcgc cggtggcgat
     5341 ctgtccctcg ggacgcagcc cgttgttgaa cggcatccac gcggccagca gcaccaggcc
     5401 cgcagcccac accgcgggct tgctcgccgc gaccgccggt cccagccggg gcagcacttc
     5461 gcgagaaagc aagagccagc acacgatccc gcagaccaga tcgggcagcc ggatccagat
     5521 actcgcgtcg ctgacactgg tcatcaacgc cagcaggttg tagtaccagc cgaacgggtc
     5581 ttcggggctg ccgaaccagc ggaagtagtt cgacatgtag ccggcgtgcc cggccacgcg
     5641 ggccatctgg agtatgtagc cgtcgtcgga tgagttcgcg ccgagcacat gccacaccag
     5701 gaagccgccg accaccacga catcggtggc ggagaacgtg cgccatcgct gcggaatcca
     5761 ccgttgcatc cggtggccgt cgagtcggtc cagccgccac aaggcgacca gcgcgacgat
     5821 cgtcgagatg atggccagca ccatcgccgc cagcttcaaa gccgttggct tggtggagaa
     5881 ccgggtgtcg atcactgctg acgcgctcaa tcccggaggc gcgggtccgg tcaggtcggt
     5941 gaagatgccg acgatcgccg gacgcaggtt cgggtcgggg aatccggtcc gctgttcctt
     6001 gccggtggtg gggtcggtca gtccgacgaa ggtggcgaag gtgcccgcct ccgacgaggt
     6061 gatctcgatg cgctgacatt cgggtgcggc cacccgcgcc ctcggcacac ttgcaatcac
     6121 cacgttgcgg tcggtgatgt cgacccgctg ggcgttgacg ttgacgaaca gcgactgcag
     6181 cgccgcctgc ttgccgtcct tcggcgcggt gctcagcacc acgccgcctg cgggcggcat
     6241 cgcccggatc acaccgcacg gcaccgtcac cgtcatagtc accggcacct gcgaaatcag
     6301 cggcgcggtg acgttattca gctgaccgtt ctgcggccag ttcagcgtcg ccgtggtttg
     6361 tacgaccggc agcaggggcg tcgcgaccga cagcacgaag ccgatgaggc ccgcgatcgt
     6421 cgccgtccac cgggcgatcc tgacgtcttg gctcatggcc cgactccggc tcgtcccctg
     6481 cccttcgggc gtgggcccac cggtcctcgc tcgttcctcg ctgcgatcct cactcgcctt
     6541 cgtcccgcgg gtcatggcaa ggccctaatc ggtccactgc ggctccaccc gaacacgcgc
     6601 gccgagccct gatcgatgac ggcgttgggc gcgaccgacg cgggtacgac ccggtcgtag
     6661 cgctcgatcc agccccagtc gcggtaccag tcgtcgcgca gatacgtcgg aacggtggag
     6721 gtgcgcagca gcgactggat gaacaggaac gggccgccgc cctcggcgga ctgccactgc
     6781 ttcgacgaca ccaccacctg cttgaagttc ggaaggatgc gatactccgg aagttcggca
     6841 acgccaaggt gttcggagaa cgggcgctga cacgggaagt tcgccgcggt ggcgatgtcc
     6901 atcagcaccg gcgtctgcga gccgaggagt tgttgtgcgg tctccagtac cggcacccgg
     6961 ggcggggtga acgcaaacca ttggtcggtc gacaggttcg gatcgtcggc gacgatgcgc
     7021 gcgacgttgg cctccggcgg cgcccacgcc agcgggaatc gcaggttgcg ccaggccttt
     7081 tgggggaaga tgtcgatcgg ctgaacctgg cccagcggct ggaagctgcc gtcgggacga
     7141 tgcacgcccc actgcagctt gagcgactgg ccgtagttga acgagccgtc ctcctcgtag
     7201 taccagatcg ccccggcagc ggcgaccgtc accaacggac gatcaggggc gcgaggcgga
     7261 agctgatacc acgccgaagt gaccttggct gcaacggtgt tctcgttgta gctgcccatc
     7321 accggcgtgc gtttggggtc gagaccgaac ggcaggtaga cgttggagcc gttgatgccg
     7381 acgggcccgt agccaccgcc agtgcccgcc gcatagccga tgccgatgtt cggctcgttg
     7441 ggagacccgg gggagttcac ggtgccgggg ttggcggtga ccggctcggc cggttccaga
     7501 gtgtcgctca ccccgtcggg tgtgaagccg accggactct ccccgccaag cggcccgtac
     7561 tggccgaagc gctgacccgg aacaggttgc agcagaccgg cattcgtatc ggcctcgacg
     7621 agcacgtcgt cggccatcgc gcagctggag ctcgacagac ccgacgtaag cgcgtcgacg
     7681 ttggccttgg cagtggtgta gatgggatag cgctgcgcgg cgcccttggc catcgaaccc
     7741 acttcgagca cgaccatgat gaccgcgacc accagcaggg gcgtcgacgc cagcacccgg
     7801 ttgcggcggg tattggcgac ctcggtgtgc ccggcgtagt ccatccggaa gtgcaaccag
     7861 ccggccagca gcccggtcgc gatcgccagc gccaggaaga tccccgtcac cgggatcccg
     7921 acgatgaccg gctgcttgtc gaaccacggc actccgtagt tgccgacgta gaaccagccg
     7981 ttgatacccg aagtggccca ggccaatacg aacagcagcg ccgtcacata tacagccagg
     8041 tttcgacggc tgtgcagccc gaccctggcg aatgcgaacg ccgtcacgcc acccaatgcg
     8101 cctgcgaggc cggcgaatgc gccgaactgc acggcccact tggtcggcgt gaaggtcagc
     8161 agcagcagac ccaccgccgt ggcgccgatc aaccgccaca ccgggccctt cgccatgcca
     8221 ggcatgccac cccgccgcag cagcaccgcc agcatgccga acaggcacag catcagcacc
     8281 agcacggcga agcgacgggt cagcgaggag tcggcgctgt cttcgaccgt aaggaagtaa
     8341 tagcgcagga agtcctgata ccaggggatc gtcgggccga ctttgtattt gatgcgggcg
     8401 gattccgcga cggtggccag cgtctggtcc cggaacatga ccacaaaaat cagcgccagc
     8461 gcggccgcca gcggagccag ctgggcgagc agtccgtcaa tcgggcgcct gctcgggatg
     8521 attcgcgcga cggctcgcgc gccgacgagt agcggggcga tcgcgatcag gccctgcggc
     8581 gccagcgtca cgctgaagac ggcgacgatg atcgcgaccg cagcgggcca cagccgccgg
     8641 gtggcgatcg cgtcctccac caggatccac gccgcgagcg caccgaatgc gatcagcggc
     8701 tcgggccgaa ggccgttgtt gaacggcagc cacgccgcca ggaacaccgc tcccgcagtc
     8761 cagaccgcga cgcggttgtt cgccagccgc ctgcccagtc gcggtagcac acagcggctg
     8821 atgatcagcc aggtcccgat tgccgccgcg gtggcgggca gccgcatcca cacgcctgcc
     8881 gtggtgatcg ccgccatgtg cgcgagcacc gactgatacc agtcgaacgg tgcctcggtt
     8941 gcaccgaaga agcggtagta gttggccgca tagcccgcgt cggcggacac ccgcgcgatg
     9001 gtcaggttgt acccgtcatc ggacgaggtc gagccgatca gatgccagag cagcagcgtg
     9061 ccgatgaccc cgacgtcgac gatccacgtc gacaggctca cccgccagaa gcgccgccag
     9121 gcgtcgcgca ccctgcgacc gcttcggcgg tccaacaccg caagggcgac gatcgacgcc
     9181 accacacaga ccacgccgag aaccatcact ccgagcttca gcgcggtcgg cgatgtgatg
     9241 aaccgggtgt ccacgtcgat gcgggcggac agccccggct gagcagccac ctgcaggtcg
     9301 gtgaacacgc cggtcacctg aggcttcttc tccgcggaga gtgtgccggt cgcgccaggt
     9361 atgccgacga agtcggcccc gacggcaccc gggttggccc agacgtgcag ggcgctgcat
     9421 gcgccggacg cgacggcagg gcgcggcgcc accgcggcca cggagtcacg gaaggcgacg
     9481 acgacgttgg cggcgttggc ggtgacgaac agcccgttgc ggctggactc gatgccgccg
     9541 ggcgggttgg tggaaagcac cacaccgcct gctgcgggca gggtggcgat cgtccggcac
     9601 ggaatcgaga cgtccaacgc ctgcggggcg ccggatacca gcggcgcggt gacgtcgctg
     9661 accagtccgt tcggccccgg ggcctgcggc cagacgatgg tcgcggtggt ctgcttgacc
     9721 ggtagcagcg gcgccagccc gcacagcagc acgcccgcaa tgcctgcgac gacggcgatc
     9781 agccgggcga tgcggtgggc aggcacgagg ctcgatggta ggcgacggac ttaatggcgc
     9841 agcggcgccg ggctccagag tccgctacga gttgccgaac cgagatccag ccgtgccggt
     9901 tgggcgttcg ggtagtacgg cgtgagctgc tgcagcgcgc cccagtcgcg gaaccagtcg
     9961 tccttcaaat aggtcggcac ggtggtgggc cgtagcagca gttcggtgat gccgagcggc
    10021 ccgccgccga ggttgtccat caccggcgag ttggcctccg cgccgaaccg gtcgggcagg
    10081 atgcgccact tcggcacctc gacgacgccg tactgatgac cgaacgggcg ctggcacggg
    10141 aacgccatgc cgaccagcca gtccagcagc acggggtcct gcgagccgac gacgtcctgc
    10201 agcgtgcgca gctcggggat ccgcggcggg gtgacagcga tccagtgctg ggggttgagg
    10261 tcgtcgtcgg tggcaaccag ccggatccgg gtggcgtcac ggggtatcgc tgccatcggg
    10321 gcccgcaggt ttcgccacgc cggggcggac ccgacgtcgg cgaagcccac gcctccagcc
    10381 ggctcgccct tgtcgttggc ccactgcacg atgacctcgc cgggatcgaa gcgcccggcc
    10441 gccgagacga cgagcagcgg gccggccttg tcgcggggcg gcagttggta ccacgcggag
    10501 cgcagcacgg ccggctgctg ggtgccgctg cgccagctgc ccatcaccgg agtgcgggcc
    10561 ggatcgaggc tgtagggcag ccgggcccgc gatccgttga cgcccgccgc ggcggtggtg
    10621 ccgccctcgg tgcctgcctc gttggcgctg accgtgccgg tttcggtgtc ggcgaagttg
    10681 aacgtgcccg gcgactccat gaccgggtcg gccgacacgt cggcgggaat gccgttgggg
    10741 ccgaagcctt ctgcagtgac cgagcccaaa gcctcgccga ccggtacgcc gatcggactc
    10801 agaagtccgg cgttggagtc ctgttcgacc atgacgtcgt tggccagccc gcacgtcttg
    10861 ccggcgagtg cttcgaggtt cgagcggccg ttgctccatc ccgggtattg gtcgatcatc
    10921 gccagtgtca gcgaaagcac ttcgaagaac accagtagcc aggcggcaat cgccaatggg
    10981 gactgcacca ttcgctgcca attgcgccgg gtcggcggcg attcgtcgcg gccggagaag
    11041 tggaaccacg ctgccaccag tagcgccagc accgacagac caagcaggaa cgtggtgaag
    11101 ccgaacctcc actcggggaa ctgattcgac catggcacac cgaagtttga gacgtaccac
    11161 cagccgttga cggtggcgaa cgacaacgcc atgacgaaca gcaccgtggc ggcgaacaac
    11221 gcccggttgc gtcgcgactt catcgccgtc gcggtgatgg ccaccgcggc caacgccccg
    11281 agcgatcccg cgaggccggc gaacacgccg aagtgatgtg tccacttggt gggggtgaac
    11341 atcatcgcca ggaacgagat gattgtgatg ccgacgatgc gtcggctcgg gccgagcgcc
    11401 gtgccgggaa tccggccctt gcgcaacgac attgccaccg aaacggccag cgccagcagc
    11461 agcgtcagca ccgcgaaccg acgcgccacc gagccgtcgg ggctggtggt gaatagccgc
    11521 tcgtagcgga tgtgctcgtc gaaccagctc aggctgggcc cgaccgcgga cttgaaggtg
    11581 ctggcctgaa tctcgccgac caacgtctgg tcgcggaaga tcaatatgat cgtcacggtg
    11641 caggcggcca ggatcggggc cagcagggcc agatggccga accgcgaagt atggcgggcc
    11701 acaatggttt tcagcggacc gaccgcgaca aggagggcgc cgacggcagc gatgcccgtc
    11761 ggcccggaga acagcgtgag cgcgccgatg actatcgcaa tggccaccgg cagcatgcgg
    11821 ttcgtcgcga cgccccgctc gaccgagcac caggtcagca gaatgccaag ggcgataatc
    11881 ggttccggcc gcagcccgtt gttcagcggc agccagaacg ccaggaacat gcccgcagcc
    11941 gtccacgctg cgacgcggct ggtcttcacc gcgtggccga gtcgcgggat gacttcgcgg
    12001 ctgatcaacc accagcacgc cagcgccatc accaaggtgg gcagccgtac ccaggcgctg
    12061 ttggtgctga cgtgcgccca cagcgccagt aggtcgtaat accagccgaa cggcgcttca
    12121 ggcgtaccga accatcggta gtagttggcc atgtagccgg cgtgctcgga cactcgggcc
    12181 atggtcagga tgtagccgtc gtcggatgtg ttggcgccca cgaagtgcca ccacaccagt
    12241 acgaccgtca cgattccgtc gagcggcgtc accgaccacc acttgcgtgg cagaacgcgt
    12301 ttgacccgcc tgccgtcggc ggtgtccagg acgtgcagcg cgccgagggc gattcccgtc
    12361 agcgcgaccc cgacgaccat cgccagcatt ttcaacagcg tcggcgacgt gctgtagcgg
    12421 gaatcgatag tcgccgagaa ctgcaggccc ggcggcgccg gtccggacag atcggtgaag
    12481 acgccgacga tctgcgggcg gaagtcgtag ccaccgcgct cgccacgcaa cggcttgccg
    12541 ggatcgtcgg aatcgcgacc cgtcaccagg ccgacgaatt cgccggcgac cttgtccgcg
    12601 tgcgcggtga acgtgagtcg ctggcacgcc gggctgagca cctggttcag cggggcgacc
    12661 accaccgggg tgttgcggac gatgacgagc agatcgttgt tgacccgttc gatgagcagc
    12721 ccgcggtcga cggctttcgg cgcctgcttc ggcaccgtcg acaacagcac cgtgcggccc
    12781 cggttctccg gccccgccag ccccgcggcc gcgctgcacg gaatggtgat gttcaagtcg
    12841 gtggcgacgt atccgatcag cggcgcgttg acgctctgca gcacgtcgtt ctgcggccag
    12901 ttgagctggg ccgtggtctg cgtcacgggc agcaggggag tggcgatcgc cagcgccgcg
    12961 ccgagcaacc cggcgacgat ggcgatgatg cgcgcggtgc gatggttgcc cgctgcggtc
    13021 acggggcttg atggtaattg gcctgtttgc ggcgccgtgt ccgtggccat cagcccgtcc
    13081 tgatcgccag cacgaagggc ccgacggtgg agacgtcgaa tcgcgggtcg tcgaacagtg
    13141 ccttgtccag tgcgacgtgg tagcggcgga cgttgggctg gttcgggtag acgtccgagg
    13201 ccagccgcag ggtgtaggcg tcgttggcgc cgcggcgcat caagaacacg gtaggcggct
    13261 tccacggcag tttgtcgagc gaggcgatga actggtcagc ggtggtcagc gtggcccagt
    13321 tttcgaccgc ggccgcgcgc tggtcgaact gcgcgagcgg atttgcgtag tgcgacgtca
    13381 gaccctggaa gccgtagtac gggtagtagg acaggaagct gtagtccgca gtcagcagaa
    13441 ccgtttcgt
//
LOCUS       DASRJA010000227        11533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2182953,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000227 DASRJA010000000
VERSION     DASRJA010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11533
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2182953"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VFC01_10175"
     CDS             <1..513
                     /locus_tag="VFC01_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZN80037.1"
                     /translation="RPDDSDGFREAWAILEQLWKSTVARAMTFPPAALHDNVDDEWSF
                     IETLRHLNFAAAAWVGRMVLGNTSPWHPLDLPWDQAPGWDGVPWNREAQPSLDEVLAV
                     RRTRQAMVRQVLETVTDDHLVTYVTRSEPGWPRMENFPLKECLLIVLNEEWQHRLYAE
                     RDLDALETAG"
     gene            complement(520..1869)
                     /locus_tag="VFC01_10180"
     CDS             complement(520..1869)
                     /locus_tag="VFC01_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN80038.1"
                     /translation="MHLGQRLNQLPTRVLSLRIIVIVAALSVVALVITLGAWVWFGVT
                     NDQYSQLDRRLDSVSSLGDISSLLLTAQNAPTNQPAPNGGLVRTARIGGTAVSVPSDI
                     VLPQFDNGYANTTINGVEYRVRTFSAGVASVAVGAPLAETQNRIDELHRRVLLICGGV
                     IVGTVLVGWVMWLIMINPFRLLAQRARAINAQSKPDEVQVRGVQEAVEIAEAVEGMLA
                     RIGDEQQRTRAALESARDFAAVASHELRTPLTAMRTNLEVLSTLNLRDEQRKEVVGDI
                     IRTQTRIEATLTALERLAQGELTTVDDFVPVDITDLLDRAAHDAIRNYPNLHVSLASS
                     TTVLMVGLPAGLRLVIDNAITNAVKHGGATEIRLSAESSSDGVEIIVDDNGDGVPEGE
                     RAAVFERFSRGTTAARSGSGLGLALVAQQAELHGGTAALEASPLGGARLTLRLPGPK"
     gene            2031..4331
                     /gene="aceA"
                     /locus_tag="VFC01_10185"
     CDS             2031..4331
                     /gene="aceA"
                     /locus_tag="VFC01_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase ICL2"
                     /protein_id="HZN80039.1"
                     /translation="MAIIEADAAPQTPFEREVAATQRYFDSPRFDGITRLYSARQVVE
                     QRGTIPTDHTVAREAAEAFYPRLRELFAQKKSITTFGPYSPGQAVAMKRMGIEGIYLG
                     GWATSAKGSISEDPGPDLASYPLSQVPDEAAGLVRALLTADRNQQYLRLQMTDEQRAT
                     TPATDFRPFIIADADTGHGGDPHVRNLIRRFVEAGVPGYHIEDQRPGTKKCGHQGGKV
                     LVPSDEQIKRLNTARFQLDTMKVPGIIVARTDAEAANLIDSRADERDQPFLLGVTNLK
                     IPSYKSCYLAMMRRFYEQGVTELNGHLLYALPDGEYAVAEAWLERHGIAGAVAEAASA
                     YQDSADHSIDAIFDKVESQFVDAWQIDAGLMTYGEAVAELLEFRESEGEQLDMSVAEW
                     RKFAQRAPMYTAREKAREVGAAVGWDCELAKTPEGYYQVRGGIPYAIAKSLAAAPFAD
                     ILWMETKTADLADAREFAEAIHAEFPDQMLAYNLSPSFNWDTTGMTDDEMRAFPEELG
                     KMGFVFNFITYGGHQVDGVASEEFATALRQDGMLALARLQRKMRLVESPYRTPQTLVG
                     GPRSDAALAASSGRTATTKSMGKGSTQHQHLVQTEVPKKLLEEWLAMWSEHYQLDETL
                     RVQLRPTRPGSDVLELGIYGERGDGDAEKLANVIVDPIKDRHGRSILTVRDQNTFAEK
                     LRQKRLMSIVHLWLIHRFKAEVVYYVTPTEDNIYQTEKMKSHGIFSDVYQEVGEIIVA
                     DVNQPRIDELLASDREALKRLIRKED"
     gene            complement(4328..4969)
                     /locus_tag="VFC01_10190"
     CDS             complement(4328..4969)
                     /locus_tag="VFC01_10190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZN80040.1"
                     /translation="MQRRLIGSGVRAHNRYADSGANGDQQRDHPGAAVCDRRGNAIPH
                     THTGTVANRTDRIGELAVGMPRITDMDASEATRDSVRAAVTVDRKGIIRQWGDAVTEV
                     VGYSADDAVGRSLNVVIPPALRPVHWWGFDQAMKSGRLNKDLFKVPALCKDGRIIVAH
                     ATIDLIAGKSGDADGAVVTFVGVGSPWQGKAWQAALAPIDVARRLWQRVRSGR"
     gene            4883..6889
                     /locus_tag="VFC01_10195"
     CDS             4883..6889
                     /locus_tag="VFC01_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN80041.1"
                     /translation="MIALLIAVGAGVCIAIVGPNTGADKPPLQLPPSAESARAAILLK
                     EFPGADQQPAILVASRRDGARLAPADLGAVEEARQRVLSRAAASAGPPLTASQDGRAA
                     VAPVPLAAGISGFALRDAITALRTSATAGAPTDLTVQVTGGPAFIADITNAMSDANIT
                     LLAVTAAVVAVLLIVTYRSPILWLVPLAVIGLADGLSTSVGTVVSRLTDLPFDGATSG
                     ITSVLVFGAGTNYALLLISRYREELSHVGEHSLALRAAVTRAGPAIVASNATVVLALL
                     ALVFAVAPATRSLGVHAACGLLVAVVFVLFVLPPLLRMFGRRLFWPFIPRRGDRTAVS
                     TGVWHRIAEWVTGHAGRVAVSTVALLALLGIGLLGTPIGLSQIDQFRVRAESVTGYET
                     LAAHFPRGLTDPTVVVGATAHAADIASVITTTPGVVTATDAGTSASGLTQWKVVLDAP
                     PASPDAFKRVAALRQSVKSVDPTALVGGSDAQALDSRDAAVRDRQVLIPAILAVVLIV
                     LYVLLRAVIAPLVLVAVMVLSALSALGVGGWASVHLLGMPALDYTTPLFAFLFLVALG
                     VDYTIFLVTRAREEATAHGTRDGVVRAVSATGPVITSAGIVMAAVFCVLAVLPLIVLT
                     QLGVIVGLGILLDTFVVRTVLIPALFTVIGPRIWWPAKVDHTFR"
     gene            6921..7745
                     /locus_tag="VFC01_10200"
     CDS             6921..7745
                     /locus_tag="VFC01_10200"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN80042.1"
                     /translation="MTARRSGVETESDAMWVPALARGLIREDVLRSMRCCDGYTDLLG
                     SSAPGRRSLAQRAMNSSMVAAIYQHIWRPIMAAAMSLHGMSISAERERATAALHLGGQ
                     QRVLDVACGPGNFTSFFAGRLAGDGFVIGLDSSEPMMERAARDNSCPRAVYMRGDALD
                     LPFNSYAFDVVSCFAALHLVSEPLRLLHEMVRVLTPGGRIAVMTTYGRESVLARKALE
                     LGATMCGVRVFDRTTVPTFFAAAGLVDIEQQLRGVSQFVVARRPKQVTEFGRWRAG"
     gene            complement(8411..11074)
                     /locus_tag="VFC01_10205"
     CDS             complement(8411..11074)
                     /locus_tag="VFC01_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80043.1"
                     /translation="MTTTETGAAQALQRAAGVELIGEMAGSGYRVPPSLVRRADGQTI
                     QLTPLLYTTLREIDGRRTAAEVAEAASVSTGRTVSEDNVQHLVDKLRPLGLLVQADGS
                     EPTLKKQSPLLALRLRYAVTDPDRTRRLTDPFRFLFRPWIVIPVLAVFAVLCWWVCFR
                     KGLAAAAYDAFERPGLLILVFVVTVLSAGFHEFGHAAAARYGGATPGVMGFGVYLLWP
                     AFYTDVTDSYRLGRKGRLRVDLGGLYFNAIVAVVITSVWLGVRYDALLLVVATQIIQM
                     IRQLAPLVRFDGYHVLADVTGVPDLYSRIKPTLLSVLPWRWGDPKARLLKPWARVVVT
                     LWVLVVVPLLLGMLATAILALPRLLATASASLSKQQAVLVSAWVDGDMVQATARVLAM
                     IAIIIPVAGVIYLLIRVTRRCAIRTWHGTAGKPMQRIVAVVFGTLVLGGVAFAWTDDF
                     RPIQQGERGTIGDILLTPFMTTTHSTASEPAPSQRVGQAPRLTAAPTLVAGQRGVMWA
                     LWDKGTPLPTKDSPQLALVLVPKSQPLSGGAFLGGGPGGYRTAEPTIAQRTITANQKD
                     QGWIFPFDKPLAPEPGDNQALAVNYRSNTVVYEAAFAFVYATDGADAMNVNEAQAYAS
                     CYNCAAVAIAYQVVFVVDTPGADDNDTDPTKDTNDNVAVPQNLSGALNYMCVNCMTYA
                     YARQLFVTLDEPLSQEAIDQLDALWEEIDDYADAIEAGTVPLGEIAPQLEAYTKQIVD
                     IVNTDQPGTIPTATASPTASPTPAPTAPSTAAAPTSQTAAPTASPTVAPTASPSLAPA
                     GSPTTQAPVTESTATATPTEASTSSPTTESSTSSPTTEPPTSFSPTDPATADPSSGTS
                     STSDPTSEPSGTTSDGSTSDGSTSGGTATSP"
     gene            11177..>11533
                     /locus_tag="VFC01_10210"
     CDS             11177..>11533
                     /locus_tag="VFC01_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80044.1"
                     /translation="MVPVDEESPLDDLSDVEAVVPAVESLDESPDDMVAVVESLVSVP
                     PSSPSREVEPSTVSGCVVPWSSEDCWLESALASPTSSWWMIAVCAETAVVSEFHVMTF
                     APTLASIGAATLALAAT"
BASE COUNT         1943 a         3711 c         3825 g         2054 t
ORIGIN      
        1 cgtcctgacg acagcgacgg tttccgtgag gcatgggcga tcctggagca actgtggaag
       61 tccaccgtgg cgcgggcgat gacgttcccc ccggcagcgc ttcacgacaa cgtcgacgac
      121 gagtggtcgt tcatcgagac gctgcggcat ctcaacttcg ccgccgcggc atgggtgggc
      181 cggatggtgc tcggcaacac ctcgccctgg cacccgttgg acctgccgtg ggatcaagca
      241 cctggctggg acggcgttcc gtggaaccgc gaggcgcaac cctcgctcga cgaggtgctc
      301 gctgtccgac gcacacgcca ggcgatggtc cggcaggtcc tagaaacagt gaccgacgac
      361 cacctggtca cctacgtcac tcgctccgag ccgggctggc cccgaatgga gaacttcccg
      421 ctgaaggagt gcctgctcat cgtcctcaac gaagagtggc aacaccggct gtacgccgaa
      481 cgtgaccttg atgcgctgga gacggcaggc tgacgccgtc tactttggtc ccggcagccg
      541 cagtgtcagg cgcgccccgc ccaacggact ggcttccagc gcggcggtac cgccgtgaag
      601 ctcggcctgc tgggccacca gcgccaggcc aaggccagag cccgaccggg ccgccgtcgt
      661 gccgcgtgag aaacgctcga aaactgcggc gcgctcccct tccggtacac cgtcgccgtt
      721 gtcgtcaacg atgatctcga cgccgtcgct ggaactttct gcgctgagcc ggatttcggt
      781 ggctcctccg tgtttgaccg cgttcgtgat cgcgttgtcg atcaccagcc gcaggcccgc
      841 gggcagcccc accatcagta cggtggtcga agacgcaagc gacacatgca gattggggta
      901 gttacgtatc gcgtcgtgcg ctgcgcgatc cagcagatcg gtgatgtcga ccgggacgaa
      961 atcgtcgaca gtggtcagtt cgccctgagc gagccgttcg agcgcggtga gcgtcgcctc
     1021 gattcgggtc tgcgtccgaa tgatgtcgcc caccacttct tttcgttgct cgtcgcgcag
     1081 attgagcgtg gacagcacct cgaggttggt gcgcatcgca gtcaacggcg tgcgcaattc
     1141 atgagaggcg accgcggcga agtcgcgggc agattccagc gcggcccttg tccgctgctg
     1201 ttcgtcgccg atccgggcca gcattccctc gaccgcctcg gcgatttcga ccgcctcctg
     1261 cacgccgcgc acctgaacct catcgggttt cgactgagcg ttgatggccc gtgcccgctg
     1321 ggccagtagc cggaacggat tgatcatgat caaccacatc acccagccca ccagaacagt
     1381 gcccacgatg acgccgccgc agatcagcaa tacgcgccgg tgaagttcgt cgatgcggtt
     1441 ctgtgtttct gccaacggcg cgccgaccgc gaccgaggcc accccggcgg aaaacgtccg
     1501 gacccgatac tcgacaccgt tgatcgtggt gttggcgtag ccgttgtcga actgcggcag
     1561 cacgatgtcg ctcggcacgg agacggcggt gcccccgatg cgggcggtgc gcaccaggcc
     1621 tccgttgggg gcgggctggt tggtgggcgc attctgtgcg gtgagcagca agctgctgat
     1681 gtcacccaga ctgctcaccg agtcgaggcg acggtctaac tggctgtact ggtcgttggt
     1741 caccccgaac cacacccacg cgccgagggt gatgaccagt gcgaccacgg aaagcgccgc
     1801 gacgatgacg atgatgcgca acgagagcac acgtgtcggc agctggttga gccgttgacc
     1861 gaggtgcact aggacgctcg ccgcgcggga ccatacatgc ctgccatctt gccggatgtt
     1921 agcggttgct gattaccgca tcgcaggcga aagtcgcgcc gtatggtcgg gatgtaaacg
     1981 acccacctgt gggccgtcgt cgaagctggc ttgaagagga gcattgaccc atggccatca
     2041 tcgaagcgga cgccgcgccg cagacaccgt ttgagcgcga ggtcgccgcc acccagcgat
     2101 acttcgatag tccgcggttc gacgggatca ctcggctcta ttcggcccgc caagtcgtag
     2161 agcagcgcgg cacgatcccc accgaccaca ccgtcgcacg cgaggcggcg gaagccttct
     2221 atccccgcct ccgcgaactg ttcgcccaga aaaagagcat caccaccttc ggcccctatt
     2281 cgccggggca ggccgtcgcg atgaagcgga tgggcatcga gggcatctac ctcggcggct
     2341 gggccacctc ggcgaagggc tcgatcagtg aagatccggg acccgacctc gccagctatc
     2401 cgctgagcca ggtgcccgac gaggccgcag gcctggtgcg cgctcttttg accgccgacc
     2461 gcaaccagca gtacctgcgc ctgcagatga ccgacgagca gcgcgccacc acacctgcga
     2521 cggacttccg gccattcatc atcgccgacg ccgacaccgg ccacggcgga gatcctcacg
     2581 tgcgcaacct gatccgcagg ttcgtcgagg ccggcgtgcc cggctaccac atcgaggacc
     2641 agcggccggg caccaagaag tgcggtcatc agggcggcaa ggttctggtg ccgtcggacg
     2701 aacagatcaa gcggctcaac accgcgcggt tccagctcga caccatgaaa gtgcccggca
     2761 tcatcgtcgc tcgcaccgac gccgaggcag cgaacctgat cgacagccgc gccgacgagc
     2821 gtgaccagcc cttcctgctc ggggtcacca acctgaagat tccgtcgtat aagtcctgct
     2881 atctggcgat gatgcggcgc ttctacgagc agggcgtcac cgaactgaac ggtcatctcc
     2941 tctacgcgct tcccgacggc gagtacgccg tcgccgaggc ctggttggag cgccacggca
     3001 ttgcgggcgc ggtcgccgag gcggcctcgg cttaccagga cagcgccgat cattcgatcg
     3061 atgcgatctt cgacaaggtc gagtcgcagt tcgtcgacgc gtggcagatc gacgcagggt
     3121 tgatgacgta cggcgaggcg gtggcggagc tgctcgaatt ccgtgagagc gagggtgaac
     3181 agctcgacat gagcgtcgcc gagtggcgga aattcgccca gcgcgccccg atgtacaccg
     3241 cccgcgagaa ggcgagggag gtcggcgccg ccgtcggatg ggactgtgag ctggccaaga
     3301 cgccggaagg ctactaccag gtccgaggcg gcatcccgta cgcgatcgcg aagtcgctgg
     3361 cggccgcccc gttcgccgac atcctctgga tggagacgaa gaccgccgat ctcgcggacg
     3421 ctcgcgaatt cgccgaggcg attcatgccg agttccccga ccagatgctg gcctacaacc
     3481 tctcgccgtc attcaactgg gacaccaccg gcatgacgga cgacgagatg cgggccttcc
     3541 ccgaagagct gggaaaaatg gggttcgtct tcaacttcat cacctacggc ggacaccagg
     3601 tcgatggcgt tgcttccgag gagttcgcca ccgcactgcg acaggacggc atgctcgcgc
     3661 tggctcgcct gcagcgcaag atgcgcctgg tcgaatcgcc ttatcggacg ccgcaaaccc
     3721 tggtgggtgg accgcgcagc gatgccgcat tggcggcgtc ctcgggtcgc acggcgacca
     3781 ccaaatctat gggcaagggc tcgacgcagc accagcacct cgtgcagacc gaggtgccga
     3841 agaagcttct cgaagagtgg ctggcgatgt ggagcgagca ttaccaactc gatgagacgc
     3901 ttcgcgtgca attgcgcccg acacgccccg gctcggatgt gctcgagctg ggcatctacg
     3961 gcgaaagggg cgacggcgac gccgagaaac tcgccaacgt gatcgtcgat ccgatcaagg
     4021 accggcatgg ccgaagcatc ctcacggtgc gcgatcagaa caccttcgcc gagaagctgc
     4081 ggcagaagcg gctgatgagc atcgtccatt tgtggctgat ccaccgcttc aaggccgagg
     4141 tcgtttacta cgtcacgccg accgaggaca acatctatca gaccgagaag atgaagtcgc
     4201 acggcatctt cagtgacgtg tatcaggagg tcggcgagat catcgtggcc gacgtgaacc
     4261 aaccgcgcat cgacgagcta cttgcttccg atcgcgaagc actgaagcgg ttgatccgta
     4321 aggaggatta gcggccggat cggactcgct gccagagtcg gcgcgcgaca tcgatcggtg
     4381 ccagcgcagc ttgccacgcc ttcccctgcc atggagatcc gacgccgacg aaggtgacga
     4441 ccgcaccgtc ggcatctccg ctcttgccgg ctatcagatc gatggttgcg tgcgcaacga
     4501 tgatccgacc gtccttgcat agggctggaa ctttgaacag atccttgttg aggcggccac
     4561 tcttcatcgc ctgatcgaat ccccaccagt gcacgggccg aagtgcgggc gggatcacga
     4621 cattgagact tcgtccgacc gcatcgtctg ctgaatagcc cacgacttcg gtgacggcat
     4681 caccccactg acggatgatg cccttgcgat cgacggtcac ggccgcgcgc acactatcgc
     4741 gggtcgcctc ggaagcgtcc atatcggtaa tacggggcat accgacggcg agttcaccga
     4801 tgcggtccgt tcggttggct accgttccgg tatgggtgtg tgggatcgcg ttgccgcggc
     4861 ggtcacacac cgccgctcct ggatgatcgc gctgctgatc gccgttggcg ccggagtctg
     4921 catagcgatt gtgggcccga acaccggagc cgataagcct ccgttgcaac tcccaccttc
     4981 tgctgaatca gcaagggctg caatattatt gaaggagttt cccggcgcag accagcaacc
     5041 ggcgattctg gtggcatccc gccgcgacgg cgcacggctg gctccggccg atttgggtgc
     5101 cgtcgaagag gctcggcagc gcgtgctgtc gcgcgccgcc gccagcgccg gcccaccgct
     5161 gacggcttca caggacggtc gtgcggcggt cgcacccgta ccgcttgcag cgggtatttc
     5221 cggatttgcg cttcgggacg cgatcaccgc gctacgcacg tccgcgacag ctggcgcccc
     5281 aaccgatctc acggtgcaag tgaccggtgg cccagctttc atcgccgaca tcaccaacgc
     5341 aatgagcgac gccaacatca cgcttctggc ggtgaccgcg gcggtggtgg cggtgctgct
     5401 gatcgtcacc tatcgctccc caatcctgtg gcttgtccca ctggcggtga tcgggttggc
     5461 cgatgggctg tccacctcgg tgggaaccgt cgtatcccgt ttgactgact tgcctttcga
     5521 cggcgccaca tcgggaatca ccagcgtgct tgtcttcggc gcgggcacca actacgcgct
     5581 gttgctgatc tcccgctacc gcgaggagct gagccacgtc ggcgagcaca gcctcgcgct
     5641 acgcgccgcg gtcactcgcg cgggaccggc catcgtggcg agcaacgcca ccgtggtgct
     5701 cgcactgctc gctctcgttt tcgccgtcgc cccggcgacc cgcagcctcg gtgtgcacgc
     5761 cgcctgtgga ctcctggtcg cggtggtttt cgtgttgttc gtgctgccgc cgctgttgcg
     5821 gatgtttggt cgcagactgt tttggccgtt cattccccgt cgtggcgacc ggacggccgt
     5881 cagcaccggc gtgtggcacc gtatcgccga gtgggtcacc ggtcacgccg gccgcgttgc
     5941 ggtgtcgacc gtggcgctgc tcgcgctgct gggcattggc ctcctcggca cgccgatcgg
     6001 gttgtcacag atcgaccaat tccgggtgag ggccgaatcg gtgaccggct acgaaactct
     6061 tgcggcgcac ttcccccgcg gtctgaccga tcccaccgtc gtggttggcg ccaccgccca
     6121 tgctgccgac attgcgtcgg tgatcacgac gacgcccggc gtggtcacgg caaccgatgc
     6181 ggggacctcg gcgtcgggac tgacccagtg gaaagtggtg ctcgacgccc cgcccgcctc
     6241 gccggatgca ttcaaaaggg ttgccgcgct gcgtcaatcg gtgaagtcgg tcgatccgac
     6301 agcgctggtc ggcggttctg acgcccaagc gcttgactct cgggacgcgg ccgtgcgcga
     6361 caggcaggtg ctcatcccgg cgattctcgc cgtcgtcctg attgtgctgt acgtgctgct
     6421 gcgtgccgtg attgccccgc tggtattggt ggcggtgatg gtgctcagtg cactatccgc
     6481 gctcggcgtc ggcggctggg ccagcgtgca cctgctgggg atgcccgccc tggactacac
     6541 caccccgctg ttcgcgttct tgttcctcgt cgccctcggc gtggactaca cgatctttct
     6601 tgtcacccgg gcgcgcgaag aagccaccgc acacggcacc cgcgacggcg ttgtgcgggc
     6661 ggtgtccgcc actggccccg tgatcacgag tgcgggcatt gtgatggccg ccgttttctg
     6721 tgtacttgct gtgcttccgc tgatagtgct cactcagctc ggagttattg tcggcctggg
     6781 cattttgctg gacacgttcg ttgtccgcac ggtcttgatc ccggcactgt tcacggtgat
     6841 cggaccgcgg atctggtggc cagcaaaagt cgatcacacg tttcggtgaa ccattcgacg
     6901 ccgtatcgcg tgacacaagt atgaccgctc gacggtcggg ggtggaaacg gaatccgatg
     6961 cgatgtgggt gccggccctt gcacgaggcc tgatccgcga ggacgtgctg cgctcgatgc
     7021 gatgttgcga cggctacacc gacctgctcg ggtccagcgc cccgggccgg cgcagcttgg
     7081 cgcaacgggc gatgaacagc tccatggtgg ccgcgatcta ccagcacatc tggcggccga
     7141 tcatggccgc ggcgatgagt ctgcacggga tgtccatttc ggcggagcgc gaaagggcca
     7201 ccgccgcact gcatctcggc ggccagcagc gggtgctcga cgtcgcctgc ggtccgggca
     7261 atttcaccag cttcttcgcg ggccggctcg ccggagacgg gttcgtcatc ggactggaca
     7321 gctcggagcc gatgatggag cgtgccgccc gcgacaacag ctgtccccgt gcggtctaca
     7381 tgcgcgggga tgcactcgac ctccccttca acagctacgc gttcgacgtg gtgagctgct
     7441 tcgccgcgct gcatctcgtg tcggagccgc tacggctgct ccacgagatg gtccgcgtgc
     7501 tcacgcccgg tggccggatc gcggtgatga caacctacgg gcgcgagtcc gtccttgcgc
     7561 gcaaggcact cgaactcggc gccaccatgt gcggggtaag ggtcttcgac cgcaccacag
     7621 tcccaacgtt tttcgcggct gccggccttg tcgacatcga gcaacagttg cgcggggtct
     7681 cccaattcgt ggtcgcgcgc cgaccgaagc aggtcaccga gttcgggcgg tggcgcgcag
     7741 gataagatcg ccggatgttc cggatcgatg ggctcaacgg cgaaagcatc gtggtcgacg
     7801 gcaactgggt cgaaagctca ggagtggatc ctcgcgcggt cgaaacccgg cggatcagta
     7861 ctccgggaca gacatcaagg agatcagccg tcggccaaag cttttcagcg tcgttcagta
     7921 aggatatgtg tgcagggcca ggctgaaaaa aaggtaggcc atgcacagca gcgagttcag
     7981 taggagcaga actaggccgg cgacgataag ccaccgcacg agtcgacgct gactgcgctt
     8041 cagcttggcg agacgctgct cgtgctgtat ctcggtcgcg gcgatcgcga aacttacatc
     8101 cgtcagttcg tcatctcgtg cgcatgcccc cgccgcgatc tgtcgaaggc gcgtcgcggg
     8161 aattgcgaca ccgactccgc agaagcgccg actcaaccgt cgcgcttgtc tgcgggtcag
     8221 cgcccgatct cgcttcagga ggggatgcga cgggcttgtc acgagcgata attttagtcc
     8281 gccggttccg gcggggtagc ggttcggcct gaggtcgtgc catccgacgt ctaaccggcc
     8341 gtattccacc cggcccatcc atccgtggcg acctaggccc tggcctagat gctacggatc
     8401 gatgtcgagg ctacggtgac gtggccgtgc cgcccgacgt ggagccgtcc gaggtggagc
     8461 catccgacgt agttccgctc ggctccgagg tggggtcgct cgtggacgac gtgcccgatg
     8521 atgggtcggc ggtggccggg tccgtcggcg agaaggacgt cggcggctct gtcgtcggtg
     8581 acgacgtcga tgactctgtc gtcggtgacg acgtcgatgc ctctgttggc gtcgccgtgg
     8641 ctgtcgactc tgtcactggc gcttgggttg ttggtgaccc tgccggtgcc agtgacgggg
     8701 acgctgtcgg agccacagtc ggggatgctg tcggcgccgc agtctgtgag gttggcgcag
     8761 ccgccgtgga tggcgctgtc ggtgctggag tcggggacgc tgtcggtgag gcagtcgctg
     8821 tcggaatcgt gccgggctgg tctgtgttaa caatgtccac gatttgtttg gtgtatgcct
     8881 cgagctgggg agcgatctcg cctaacggca ccgtccccgc ctcaatggcg tccgcgtagt
     8941 cgtcgatctc ctcccatagg gcgtcaagtt ggtctatcgc ctcttgggac agcggctcgt
     9001 cgagtgtgac gaacagctgc cgcgcgtacg cgtaggtcat gcagttgacg cacatgtagt
     9061 tgagggcacc ggacaggttc tgcggcaccg ccacgttgtc gttggtgtct ttggttggat
     9121 cggtgtcatt gtcatcggcg ccaggggtat ccacgacgaa gaccacttgg taggcgatcg
     9181 ccaccgcggc gcagttgtag cacgacgcgt aggcctgtgc ctcgttgacg ttcatagcgt
     9241 cggcaccgtc ggttgcatag acaaacgcaa aggctgcctc gtacaccaca gtgttgcttc
     9301 tgtagttgac ggccagcgct tggttgtcgc cgggctccgg ggccaacggc ttgtcgaatg
     9361 ggaagatcca gccctggtcc ttctggttcg ccgtgatcgt gcgctgggca attgtcggct
     9421 ccgccgtgcg ataacccccg gggccgccac ccaagaaggc gccacccgac aagggttgcg
     9481 acttgggcac cagtaccagc gcgagctgtg gcgagtcctt cgtcgggagc ggggtcccct
     9541 tgtcccacag cgcccacatg acgccccgct gtcccgcgac gagggtcgga gctgcggtca
     9601 ggcgcggtgc ttggccgacc ctctgcgacg gcgcgggctc gctggcggtc gagtgcgtgg
     9661 tggtcatgaa gggtgtcagg aggatatcgc cgattgtgcc gcgctcccct tgctggatgg
     9721 gccggaagtc gtcggtccat gcgaaggcga ccccacccag gaccagagtg ccgaacacga
     9781 cggcgacaat gcgctgcatc ggcttgccgg ctgtgccgtg ccaggttcgg atggcgcaac
     9841 gccgggtgac acggatcagc aggtagataa cgcccgccac tgggatgatg atcgcgatca
     9901 tcgccagcac cctggctgtg gcctggacca tgtcgccgtc gacccacgcc gagaccagca
     9961 ccgcctgctg tttgctgagg ctggccgagg ccgtggcgag caggcggggc agcgcgagga
    10021 ttgccgtggc gagcatgcca agcagcagcg gaaccacgac tagcacccat agggtcacca
    10081 cgacgcgggc ccacggtttg agcaggcgcg ccttcggatc gccccaccgc cagggcaaca
    10141 cactgagcag ggtcggcttg atgcgagagt acaggtccgg gacgccggtg acgtccgcca
    10201 gaacgtggta gccatcgaac ctcaccagcg gcgcgagctg gcggatcatc tggatgatct
    10261 gcgtggccac gacgagcagc agggcgtcgt atcgcacccc caaccacacg ctggtgatga
    10321 cgaccgcgac gattgcgttg aagtaaagtc cgccgaggtc gacgcgcaac cgtcctttgc
    10381 ggcccaggcg gtaggagtcg gtgacgtcgg tatagaacgc gggccacagg aggtaaacgc
    10441 cgaagcccat gacgccgggc gttgcgccgc cgtaccttgc ggccgcagcg tgcccgaact
    10501 catggaagcc ggccgacagc acggtgacca cgaagaccag gatcagcagt ccggggcgtt
    10561 cgaaggcgtc gtaggccgcc gcggcgaggc ccttgcggaa gcacacccac cagcacagaa
    10621 ccgcgaacac ggctaggaca gggatcacta tccagggtcg gaacaggaag cggaacggat
    10681 cggtgagccg ccgtgtccga tcagggtcgg tgacggcgta gcggagccgc agggccaaca
    10741 gcgggctctg cttcttcagc gtcggttcgc tgccgtcggc ctggaccaag aggccaagag
    10801 gccgcagctt gtcgacgagg tgctggacat tgtcctcgct gacggtccgg ccggtcgaca
    10861 cggaagccgc ctccgccacc tctgccgcgg tacgacgacc gtcgatctcg cgcaaggtcg
    10921 tgtacaggag gggggtgagt tggatggtct ggccgtcggc gcgacggacc agggacggcg
    10981 ggacccggta gccggatccc gccatctcgc cgatcagttc gacaccggcc gcgcgttgca
    11041 gggcttgcgc cgcccctgtc tcggtggtcg tcatagtcac acgagtgctc ccagtcgtgt
    11101 cagcagatca agcagtgcct cagtgcgtta ggcgccggaa tccctgctcg agctgccgga
    11161 cgttgaccca ctcgaagtgg ttccggtcga cgaggaatca ccgctcgacg acctgtcaga
    11221 cgtcgaggcg gtcgtgcccg ccgtcgagtc gctcgacgag tcgccggacg acatggtcgc
    11281 cgtcgtggag tcgctggtgt cggtgccgcc cagctcgccc agccgcgagg tcgagccgag
    11341 caccgtatca ggctgtgtag tgccctggtc gagcgaagat tgctggttgg agagtgcatt
    11401 ggcctcgccg acgagctcct ggtggatgat cgcggtctgt gccgagaccg cggtcgtgtc
    11461 ggagttccac gtgatgacgt tcgcgccaac gctggcgtcg ataggcgcgg ccacgttggc
    11521 gttggcggcc aca
//
LOCUS       DASRJA010000228        27604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1861110,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000228 DASRJA010000000
VERSION     DASRJA010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 27604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27604
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861110"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VFC01_10215"
     CDS             <1..850
                     /locus_tag="VFC01_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZN80045.1"
                     /translation="FEARIPRDKAPIADVTGRVQLEEGDRFYKITIVPDDGGEEVVYD
                     KLSRRQRLKVFKHDDGTERLLADGDHVEVGQQLMEGSADPHEVLRVQGPREVQIHLVK
                     EVQEVYRAQGVSIHDKHIEVIVRQMLRRVTIIDSGATEFLPGSLTERGEFETENRRVV
                     AEGGEPAAGRPVLMGITKASLATDSWLSAASFQETTRVLTDAAINCRSDKLQGLKENV
                     IIGKLIPAGTGINRYRNIQVQPTEEARAAAYTIPSYEDQYYSPDFGQATGAAVPLDDY
                     GYSDYR"
     gene            971..1378
                     /locus_tag="VFC01_10220"
     CDS             971..1378
                     /locus_tag="VFC01_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZN80046.1"
                     /translation="MMRHVAMLVSSAALTAGVLTACGGDDKSSSPPNTVGQSPTIETP
                     YTTTTAQPAGPEIVISGMAYTVPPSVKPGERITVVNKDDPNHTVTADANNLFDVRISG
                     GGGISTLIAPSTPGTYPFHCKYHAGMQGSLIVQ"
     gene            1430..1657
                     /locus_tag="VFC01_10225"
     CDS             1430..1657
                     /locus_tag="VFC01_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80047.1"
                     /translation="MNIVVVVVAVVLMVIVLIATGLLLRSVVGSPKQGYRRNLDDIRR
                     IRNDIRAEDPNASKISVNRDAWAGTSTDLPG"
     gene            1702..2454
                     /locus_tag="VFC01_10230"
     CDS             1702..2454
                     /locus_tag="VFC01_10230"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HZN80048.1"
                     /translation="MLIGSHVHQDDPLAAAEADGADVVQFFLGNPQSWKKPKPREDAE
                     LLKASSMPLYVHAPYLINVASANNRVRIPSRKILQDTCDAASEINATAVIVHGGHADD
                     NDIEAGFERWVKALQYLETDVQVYLENTAGGDHAMARHFDVIGRLWDHIGDSGIGFCL
                     DTCHAWAAGEALIDAVDRIKALTGRIDLVHCNDSRDAAGSGADRHANFGTGQIDPELL
                     VAVVKAADAPVICETADEGRKDDIAFLREHVG"
     gene            2522..3592
                     /locus_tag="VFC01_10235"
     CDS             2522..3592
                     /locus_tag="VFC01_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZN80049.1"
                     /translation="MSAVDDSPIALVRLQDADQLRERRLTIARRIAIATWAAVIVYRT
                     VTTGFAFNRELLLLYICTGLLVASIGQGRRMLYVIRDWLPFAVVLVAYDLSRGAATLI
                     GRPTMWHWQIDADRWLFFGTVPTVWLQERLKLPFPPWWEIVISTVYMSFFILPYVVAA
                     VLWLRDREEWKAFVRLFVGLSFTALFIYALLPAAPPWAAARCTSTDVAGGPSGPRCMF
                     GPARGVPDGGLLGAMQTTADGANGWVERIVGRGWGKLNLQTASALIDQGQASVNLVAA
                     IPSLHAGLTAAVAAFLWTRVGRIWRLLLVAYVLVMAFTLVYTAEHYVVDILLGWVLAA
                     IVLVVVRQYEVRRRRRAEKAYG"
     gene            3670..4062
                     /locus_tag="VFC01_10240"
     CDS             3670..4062
                     /locus_tag="VFC01_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80050.1"
                     /translation="MLTGSRVHPLRPRALIAQIAIYVVIALAAGGCFFFDVPSEVHII
                     SVGGMQRTYRLYTPPNLPPNAPLVVVLHGALANTINAETTSGFDQRARIGKFLVAYPD
                     ESTRPGTRTAAAETRRKRTSTTSPSSPR"
     gene            4125..4517
                     /locus_tag="VFC01_10245"
     CDS             4125..4517
                     /locus_tag="VFC01_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80051.1"
                     /translation="MLAHTMACRTDLFAVIGVVAATMFDPCPDPRPTSVMHIQGMADN
                     LVKFNGTPKGDTATVDGPPIPDVNAFWRRVDDCAPPEVSKNETVTITAAKCPDNREVV
                     LVTVDDLGHKWPDNAAAAFWKFFERHPK"
     gene            4566..6194
                     /locus_tag="VFC01_10250"
     CDS             4566..6194
                     /locus_tag="VFC01_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80052.1"
                     /translation="MPDTHVVTNQVPPLDSYNPATSPVLTEALIREGGEWGLDEVTEL
                     GALSGSRQAQRWGELADRNQPILRTHDRYGHRIDEVEYDPAYHELMKVAVGHGLHAAP
                     WADDRPGAHVVRAAKTSVWTPEPGHICPISMTYAVVPALRNNPDLAAIYEPLLASREY
                     DPELKVPSTKVGITAGMSMTEKQGGSDVRAGTTQATPNGDGTYRLVGHKWFTSAPMCD
                     IFLALAQAPGGLSCFFLPRILPDGSRNRMFLQRLKDKLGNHANASSEVEYDGATAWLV
                     GEEGRGVSTIIEMVNLTRLDCTLGSATSMRSGLTRAIHHAQHRKAFGAYLIDQPLMRN
                     VLADLAVEAEAATIVAMRMAGATDAAVRGDERETLLRRIGLAASKYWVCKRATPHAAE
                     AMECLGGNGYVEESGMPRLYREAPLMGIWEGSGNVSALDTLRAMATRPECVDVLFDEL
                     AKTTGQDPRLDAHVDTLKPALEDLETIQYRARKIAEDISLALQGSLLLRHGHPAVAEA
                     FLATRMGGAWGGAYGTMPTGLDLAPILERAMVKG"
     gene            6191..7129
                     /locus_tag="VFC01_10255"
     CDS             6191..7129
                     /locus_tag="VFC01_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZN80053.1"
                     /translation="MTHAIRPVDFDNLKTMTYEVTDRVARITFNRPEKGNAIVADTPL
                     ELSALVERADLDPGVHVILVSGRGEGFCAGFDLSAYAEGSSSAGGGSPYKDTVLSGKT
                     QAINHLPDQPWDPMIDYQMMSRFVRGFSSLMHADKPTVVKIHGYCVAGGTDIALHADQ
                     VIAAADAKIGYPPTRVWGVPAAGMWAHRLGDQRAKRLLLTGDCITGAQAAEWGLAVEA
                     PEPKDLDERTERLVERIAANPVNQLIMVKLAMNSALYNQGVANSAMISTVFDGIARHT
                     PEGHAFVAQSREHGFREAVRQRDEPFGDYGRKASGV"
     gene            7150..7863
                     /locus_tag="VFC01_10260"
     CDS             7150..7863
                     /locus_tag="VFC01_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZN80054.1"
                     /translation="MTARSVVLSVLLGAHPAWATASELIRLTADFDLREPTVRVALTR
                     MVSAGDLVRSEDGYRLSDRLLARQRRQDDALNPQVRKWNDSWMTLVITSVGIDARTRA
                     ALRTTLQDKRFAELREGVWLRPDNLDMDLTPDVLDRVRVLRSRDDDSSELAARLWDLP
                     GWVRTGHHLLDEIATATDIPGRFVAAAAIVRHLLTDPVLPDELLPDDWPGDALRTAYT
                     NFAAELVARRDGVELMEAT"
     gene            7875..8627
                     /locus_tag="VFC01_10265"
     CDS             7875..8627
                     /locus_tag="VFC01_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZN80055.1"
                     /translation="MRVESNGPVTTVIMNRPEARNAVNGAAAAELYEAFDEFDKDDSA
                     SVAVLWGDNGTFCAGADLKALGTADANPVHRTGPGPMGPSRMVLSKPVIAAVSGYAVA
                     GGLELALWCDMRVVEEDAIMGVFCRRWGVPLIDGGTVRLPRIVGHSRAMDMILTGRGV
                     DANEAYAMGLANRVVPKGQARQRAEELAAELAQLPQQCMRSDRLSMLNQWGSSEAEAM
                     DFEFASISRVAGESLEGAARFAAGAGRHGASA"
     gene            complement(8624..9019)
                     /locus_tag="VFC01_10270"
     CDS             complement(8624..9019)
                     /locus_tag="VFC01_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HZN80056.1"
                     /translation="MSIRLFAAVALAALVLAGCGSESKETTTPTATAGSSGAQVEIGN
                     TINYGSFGTTADIDCADGKSLNIGGSNNTLTVKGTCANVNIGGADNKVTFEKVDKEIS
                     VVGLNNTVTYKDGDPKINDTGSNNKISKG"
     gene            complement(9016..9393)
                     /locus_tag="VFC01_10275"
     CDS             complement(9016..9393)
                     /locus_tag="VFC01_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HZN80057.1"
                     /translation="MTSKAVGRSLAACAVAFPVAFGLGAPSAQAANGDTHITGNGITQ
                     TIDCNESTLFVLGTGNTVYALGSCWAVTVQGSSNVVVADNIVDNVIVYGSDQTVFYKS
                     GEPVVWDRGRELGMVNRVDRVAA"
     gene            9481..10014
                     /locus_tag="VFC01_10280"
     CDS             9481..10014
                     /locus_tag="VFC01_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HZN80058.1"
                     /translation="MRWRVNPVAVVAVTAVVAVAGCSQTVSGTARRSQPGVPDPNRSY
                     GYVDDRCGLLVDGSIQQTIGADHIVRPYSGAVCQYVLARGSAMVDVTFSWYETGNLDR
                     ERRLAAQRGATITDKIVERHQAFLASRDTTGAACSATAAAGTGVLSWWVQFRGQHNGD
                     PCKDAEKLLTATLQSDM"
     gene            10017..10550
                     /locus_tag="VFC01_10285"
     CDS             10017..10550
                     /locus_tag="VFC01_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HZN80059.1"
                     /translation="MALLRSTCWAALAVVAALLVGCGPSENQSAPPATPSAPPDAGFR
                     SADCNGVTDADVTKAVGSSMFTKAVVSDAGCFWQENTVLGTFGQGMGISTWWYRGSDM
                     DTERSLEQQAGRTLTELTIDGNKGFKAYDANACSIYVAKGTDVITWSIQTMNPAMLPD
                     LCTITGQLAELSQERVN"
     gene            10619..11206
                     /locus_tag="VFC01_10290"
     CDS             10619..11206
                     /locus_tag="VFC01_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HZN80060.1"
                     /translation="MAAPQPAGARRSTRLSRDSIVNAALTFLDREGWDALTINALATQ
                     LGTKGPSLYNHVHSLDDLRRTVRMRVVGDIIDMLNTVGQGRTRDDAVTAMAAAYRSYA
                     HHHPGRYSAFTRMPLGGDDPEFTEATRAAAAPVISVLASYGLDGESAFYAALEFWSAM
                     HGFVLLEMTGAMNGIDTDAVFTDMVMRLASGMERR"
     gene            11475..11849
                     /gene="rpsL"
                     /locus_tag="VFC01_10295"
     CDS             11475..11849
                     /gene="rpsL"
                     /locus_tag="VFC01_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007469952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZN80061.1"
                     /translation="MPTIQQLVRKGRRDKIAKVKTAALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVKLTSQVEVTAYIPGEGHNLQEHSMVLVRGGRVKDLPGVRYKIIRGSL
                     DTQGVKNRKQARSRYGAKKEKS"
     gene            11849..12319
                     /gene="rpsG"
                     /locus_tag="VFC01_10300"
     CDS             11849..12319
                     /gene="rpsG"
                     /locus_tag="VFC01_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZN80062.1"
                     /translation="MPRKGPAPKRPLVNDPVYGSQLVTQLVNKVLLQGKKSLAERIVY
                     GALEQARDKTGTDPVVTLKRALDNVKPALEVRSRRVGGATYQVPVEVRADRSTTLALR
                     WLVSFSRQRREKTMVERLANEILDASNGLGASVKRREDTHKMAEANRAFAHYRW"
     gene            12421..14526
                     /gene="fusA"
                     /locus_tag="VFC01_10305"
     CDS             12421..14526
                     /gene="fusA"
                     /locus_tag="VFC01_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZN80063.1"
                     /translation="MAQKDVLTDLSKVRNFGIMAHIDAGKTTTTERILYYTGINYKIG
                     EVHDGAATMDWMEQEQERGITITSAATTTFWKDNQLNIIDTPGHVDFTVEVERNLRVL
                     DGAVAVFDGKEGVEPQSEQVWRQADKYDVPRICFVNKMDKVGADFYFSVRTMEERLGA
                     NAVPIQLPIGAEGDFEGIVDLVEMNAKVWRGETKLGETYETIEIPADLAEQADEYRTK
                     LLEVVAETDEALLDKYVGGEELTVEEIKGAIRKLTINSEIYPVLCGSAFKNKGVQPML
                     DAVVDYLPSPLDVPPAVGHLPGKEDEEIVRNATADEPFAALAFKIATHPFFGKLTYIR
                     VYSGTVESGSQVINATKGKKERLGKLFQMHSNKENPVDRASAGHIYAVIGLKDTTTGD
                     TLSDPNQQVVLESMTFPDPVIEVAIEPKTKSDQEKLGTAIQKLAEEDPTFKVHLDQET
                     GQTVIGGMGELHLDILVDRMKREFKVEANVGKPQVAYKETIKRMVDKVEYTHKKQTGG
                     SGQFAKVLISIEPFTGEDGATYEFENKVTGGRIPREYIPSVDAGAQDAMQYGVLAGYP
                     LVNLKVTLLDGQYHEVDSSEMAFKVAGSQALKKAAGQAQPVILEPIMAVEVTTPEDYM
                     GDVIGDLNSRRGQIQAMEERAGARVVKAHVPLSEMFGYVGDLRSKTQGRANYSMVFDS
                     YAEVPANVSKEIIAKATGQ"
     gene            14612..15802
                     /gene="tuf"
                     /locus_tag="VFC01_10310"
     CDS             14612..15802
                     /gene="tuf"
                     /locus_tag="VFC01_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZN80064.1"
                     /translation="MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKYPELN
                     ESRAFDQIDNAPEERQRGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMD
                     GAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADAVDDEELLELVELEVRELL
                     AAQEFDEDAPVIRVSALKALEGDAKWVESIEELMSAVDESIPDPVRETDKPFLMPVED
                     VFTITGRGTVVTGRVERGVVNVNEEVEIVGIRPDTTKTTVTGVEMFRKLLDQGQAGDN
                     VGLLLRGIKREDVERGQVVVKPGTTTPHTEFEGQVYILSKDEGGRHTPFFNNYRPQFY
                     FRTTDVTGVVTLPEGTEMVMPGDNTDISVKLIQPVAMDEGLRFAIREGGRTVGAGRVT
                     KIIK"
     gene            16333..17358
                     /locus_tag="VFC01_10315"
     CDS             16333..17358
                     /locus_tag="VFC01_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80065.1"
                     /translation="MSIVFRSPDGNGGTNETRINVLHGNIFDPQLGLLGANVSNNSTT
                     PNGTASTANNPLVNNLIFNNPITQFLNSLLNAEIVFGGAAAGPINNSTQISLFSFNIF
                     NPQASFFGPNVGNNTAITNYAENNGNYSQTSVTSGGLLGAVVRLFLGGGMTGNGNTTQ
                     IAGGTSNITNPQVSIGGSNVSNNTAVTNEAIGNGNSSQTTVGGSGNVISVGTTGNGNT
                     NQIAVGTSNIENTQVSIGDVGLSPRPEELSANQSGTQLATTSTTPTSSSVSSTDSQSS
                     SSIGTASLTDTSGSSAPESGSVTSGSGGTTGTGGTTGTSGSGGTSGSGGSGDSGGSGG
                     SSGSGGE"
     gene            17470..18264
                     /locus_tag="VFC01_10320"
     CDS             17470..18264
                     /locus_tag="VFC01_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80066.1"
                     /translation="MSKHRKARKAPMPVKLNRVIAIGAVAGSTVGFGALLAANPPMAY
                     ADESSWWLLSGNNTPIASDNNIVIGTNGNGNTNQLAFGTGIIGNAQINLFSLGVATVA
                     NAAPAVGGNAIGGPALSTATEAGVPIGGLAVGGAAVNPAVGINPALGGIGGLGTALGG
                     PAIPVALGGLATQVATGTQANTAPGGDATGGSATNAGKATSGRATGGTAIGGNATTSA
                     SGVGSGNLSNNNSSTNTSTGNGNSSSSSNGGTNDGSSSGGTSSSGG"
     gene            18397..19251
                     /locus_tag="VFC01_10325"
     CDS             18397..19251
                     /locus_tag="VFC01_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN80067.1"
                     /translation="MFRRITTIACTAAMAGAAFAGLSAGTASAVPGCPDVHWIGAAGS
                     GERTPAEVTQNGGMGRVVYQSLQELSAEAQKDGRTMTAEAVEYPAVEAPGEDGGIGEW
                     LGFMGSVDTGAAALSRQYEAFVQQCPTSKVVLAGYSQGAMVVHRNLAALEASPNLAAA
                     LLVADGDRLPADPTLNFGTANGIPERGKGVAQDWPILAHAPAPLSPAMGARTISVCDL
                     NDAVCDYDEDADDSTGAYARRVVVHTSYARTPGLAWTAPLHFLLGPAPASPEVHEVTP
                     LSATAPTQ"
     gene            complement(19226..20608)
                     /locus_tag="VFC01_10330"
     CDS             complement(19226..20608)
                     /locus_tag="VFC01_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN80068.1"
                     /translation="MTATDEPVRLRWTLAAVSMALFCVQIDYFAMNLALPRMAFDLNS
                     TAADLQWVISVYMLALGSFMVPAGRIGDIFGRRRALLAGIVLFGLSSALCALAPSATL
                     LVAFRAVQGIGAALIFPVSVSVLTNAFPSVRASHAIGLAYGIAGLGNAAGPLIGGLLT
                     ETVGWRWIFWLNVPLTLIALAIGAWSITESFDETVPRRIDVTGLALITVGIGLFTLTF
                     DRAPSWGWLSAPTVIAFVCSLAALAFFVVAENRVRWPLVDLSLMRNPRFTILVIAGTI
                     ANIAYGVTIFLSTMYLQQVRGLDPLTAGLVFLGPSAGAALGGVLAGRLAASRPPVLVM
                     GLTSVAAAMSLAALASSHTWVVYLPALTACGLTLGLVYAFTTVATQAVVRTERAGEAA
                     GVTLTALVTLAGVGVAVSATVLEMLQRAGMSTAGAVDTIVAVLAALLLPAGVVVLLIA
                     RAATESAQSR"
     gene            20688..21533
                     /locus_tag="VFC01_10335"
     CDS             20688..21533
                     /locus_tag="VFC01_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZN80069.1"
                     /translation="MLGRYIKSQIMVLVCGGLVGPIFLGVYFATGQSGILKWMFWVGL
                     LITAADVLIALALANFGAKSEAKTQALEQSGVLALAQVVGIQETGTRINEQPLVKLDL
                     QISGPGITPFATQDRVIASITRLPMITNRKLVALVDPATNEYQIDWERSALVGGVMPA
                     NFTIAEDNKTYDLSGQAGPLMEILQILKANGIGMNNMIDLRSNPAARAQVQAVVRRAA
                     AAQQAPAAAAPAAAPAAAPGAPYAATPEPSTAQRLQELETLRATGVISDDEYNAKRQQ
                     ILSDL"
     gene            21614..23056
                     /locus_tag="VFC01_10340"
     CDS             21614..23056
                     /locus_tag="VFC01_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005275 - amine transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HZN80070.1"
                     /translation="MTATEQPEKVTEKVSLPTLTAMVVGSMIGSGVFLLPRRFGTETG
                     VLGAIIAWTIAGSGMLMLAFVFQRLATRKPDLDAGIFAYAKAGFGDYVGFNSAIGYWA
                     SACAGNTSYWVLITATMSAVVPGFGDGDTVLAVVVSAIAVWLFVYLVLRGVKDAAVIN
                     YIVTIAKVVPILMFIVITAIAFKADVFADNFWGGDDYSFSSIWDQATGTMLITVFVFL
                     GIEGASVYSRMARKREDVGRATIIGFLSVLSVFALVTLVSYGVMPQSELAGVSQPSMA
                     AVLESVVGHWGSIFIRIGVIVSVLGAYLAWTLMAAEILYIPAKTDDMPRFLARTNSHG
                     APVTALIMAAGLISLLLIALLFAADALDFMLDLTAALSLIPYLLAAAYALKLTATRET
                     YGDGKSLVPDMVIAAVATVYTLFLIYAAGVDKLLLSCILYAPAAALYFKARRERGLRV
                     FKPAEAVLFGVIVLGAVAGVVSLATGAIEI"
     gene            23083..24327
                     /locus_tag="VFC01_10345"
     CDS             23083..24327
                     /locus_tag="VFC01_10345"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HZN80071.1"
                     /translation="MTNPSTNYGVHSEVGKLRKVLVCSPGLAHERLTPTNCDDLLFDD
                     VLWVQNARRDHFDFIDKMRDRDVEVVELHDVLTETMKDPAAKAWLLDRKIVPNEVGLG
                     LVDDTRGYLDSLQPRRLAELLIGGMSTRDLPKELQSGYRALARESTGVTEYLMPPLPN
                     TLYTRDTTCWVYGGVTLNPLFWPARHDETLLMKAIYEFHPDFVGSTVWWGDPEEQWGM
                     ATFEGGDVLVPGNGVVIIGMSERTSRQAITQVASRLFAEEAATQVIVAGMPKLRAAMH
                     LDTIFTFADRDVVTIYPDIVDAMQTFTLHPSDNDVGVEVVEADKPFVEVVAAGLGLGK
                     LRVVETGGTAYDSERQQWDSGNNLVAVEPGVVFAYDRNTHTNTLLRRAGIEVITIVGA
                     ELGRGRGGGHCMTCPLARDSVY"
     gene            24399..25253
                     /locus_tag="VFC01_10350"
     CDS             24399..25253
                     /locus_tag="VFC01_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZN80072.1"
                     /translation="MTATGGPLEGRVAFITGAARGQGRAHAIRLASEGADIIAIDICG
                     PISETITYPLGTSEELAETVAAVEATGRKVLAREVDIRDLPALQQVVADGVEQFGRLD
                     IVVANAGVLSWGRMFEMSEEQWDTVIDVNLNGTWRTIRAAVPAMIEAGNGGSIIIVSS
                     SAGTKATPGNGHYSASKHGLVAITNALAIEVGEFGIRVNSIHPYSIDTPMVEKEAMMA
                     VFAKYPQYLHSFSPMPYRPVNHVGKKGLQEFMTAEEVSDVVAWLAGDGSATISGSQIA
                     VDRGTAKY"
     gene            complement(25254..26135)
                     /locus_tag="VFC01_10355"
     CDS             complement(25254..26135)
                     /locus_tag="VFC01_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZN80073.1"
                     /translation="MTVAVGYLAGKGGRSPLYLAVEAAKTLKTSLTVVTVVPRPWMTP
                     SLGRIDAEYAQYAEQLAANSAREAQGCIDSIGQGLDVKYHKVAHRSTTGGLLEAVSEL
                     EPEVLVLGSASDGKLGQVVVGSTADRLLHSSPVPMAISPRGYRGSKAGGLTRITAAYP
                     GTPEALHVVERVAALAERLSVPMRVVTFAVRGRTMYPPEVGLSTEDSILDQLAAHARE
                     TLAQLKTDGVIGKDVELQVVTGNGWDEALDEADWQEGDLLAIGTSPVGGIARVFLGSR
                     GSKILRHSPVPVLVLPG"
     gene            complement(26132..>27604)
                     /locus_tag="VFC01_10360"
     CDS             complement(26132..>27604)
                     /locus_tag="VFC01_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN80074.1"
                     /translation="PIAHGASDHLKRSIGTFQLTLFGVGATVGTGIFFVLSAAVPEAG
                     PAVLVSFVLAAIAAGLSAICYAEMASSVPVSGSTYSYAYTTMGEFVAMGVAACLLLEY
                     GVSISAVAVGWSGYLNKLLDNLFGWQLPQSLTAAPWDETPGIVNLPAVLLIVMCMLLL
                     IRGASESALVNTIMVLIKLGVLGMFIIIALTAFNADHFHGFWDKGMAGITTAASMIFF
                     SFIGLDAVSTAGDEVKDPQKTMPRAIIAALIVVTSFYILVAFAGLGTQDAAEFGSEEQ
                     SEAGLSVILENITGGTWASTLLAAGAVISIFSVTLVVMYGQTRILFAMGRDGLLPSMF
                     AKVNPRSMTPVGNTIIVAVATGILAGFIPLNWLLDAVSIGTLVAFITVSVGVIILRVR
                     EPNLERPFKVPGYPVTPVLSVLACAAVLYGLRWQTWLVFGACVGLVLLFYLFWGRRHS
                     ALNAGGADGYIPTAVPGDDDVMFVEEPSDHHFVTPHKDEP"
BASE COUNT         5280 a         8885 c         8869 g         4570 t
ORIGIN      
        1 gttcgaggct cgcatcccgc gcgacaaggc gccgatcgcc gatgtcaccg gacgtgtgca
       61 gctcgaagag ggcgaccgct tctacaagat caccatcgtt cccgatgatg gtggcgagga
      121 agtcgtctac gacaagctct cgcgtcgtca gcgcctgaag gtgttcaagc acgacgacgg
      181 caccgagcgg ctgcttgccg acggcgacca cgtcgaggtg ggccagcagc tgatggaagg
      241 ctcggccgat ccgcacgagg tgctgcgtgt ccagggcccc cgcgaggtgc agatccacct
      301 cgtcaaggag gtccaggagg tctaccgcgc tcagggcgtg tcgatccacg acaagcacat
      361 cgaggtgatc gtccggcaga tgctgcgtcg cgtcacgatc atcgattcgg gtgcgacgga
      421 gttcctgccc ggctcgttga ccgagcgtgg cgagttcgag accgagaacc gtcgggtggt
      481 tgccgagggc ggcgagcccg cggctggtcg cccggtgctg atgggcatca cgaaggcgtc
      541 gctggccacc gactcgtggc tgtcggcggc gtcgttccag gagaccacgc gcgtgctgac
      601 cgatgcggcg atcaactgcc gcagcgacaa gctgcagggt ctgaaggaga acgtgatcat
      661 cggcaagctg atcccggccg gcaccggcat caaccggtac cgcaacatcc aggtgcagcc
      721 gaccgaggag gcccgggccg ccgcgtacac gatcccgtcc tacgaggatc agtactacag
      781 cccggacttc ggccaggcca ccggtgccgc ggtgccgctg gacgattacg gctacagcga
      841 ctatcgctag tcaaaacgaa aaagcccccg gtatccccgg gggctttttc tcttgcggtt
      901 tgtgcgccga acggcctgtg ttggtgcgat gctgggtctg cgcgcgtcat cgctatctag
      961 gggggaaaac atgatgcgac acgtcgcgat gctggtttcg tccgctgcgc ttaccgcggg
     1021 cgtgttgact gcgtgcggcg gcgacgacaa atcgtcttct cctccaaaca cggttggcca
     1081 gtcgccgaca atcgagacgc cgtacacgac gaccaccgcc caacctgcgg gcccggagat
     1141 cgtgatctcc ggcatggcct acacagtgcc cccgtcggtg aagcccggcg agcggatcac
     1201 cgttgtcaac aaagacgacc cgaaccacac cgttacagcc gacgcaaaca atctgttcga
     1261 cgtccgaata tccggcggcg gcggcatatc gacactcatt gcgccgagca cgcctggcac
     1321 ctacccattt cactgcaaat accatgccgg tatgcaggga tcgctgatcg tccagtagca
     1381 ccgacgtctg aacgcgagcc cgtgtctgct ggcggcgtag ccttttgcta tgaacatcgt
     1441 ggtcgtggtg gtcgccgtgg tgctgatggt catcgtcctg atcgcgaccg gcctgttgct
     1501 ccgcagcgtc gttggctcgc ccaagcaggg ataccggcgc aatctggacg acatccgacg
     1561 gatccgcaac gacattcgtg ctgaggaccc caatgcgtcg aagatcagtg tgaatcgcga
     1621 cgcctgggcc ggcacctcga cagacctgcc cggataaccg acactcgccg ggctgtcggg
     1681 ccgcctcact agactggccg cgtgcttatt ggttcgcacg tccaccaaga cgatcccctg
     1741 gccgcggccg aggccgacgg cgccgacgtg gtgcagttct tcctcggcaa cccgcagagc
     1801 tggaagaagc ccaaaccccg cgaagacgca gaattactga aggcgtcgag catgcccctg
     1861 tacgtgcacg cgccctacct gatcaacgtg gcgtcggcca acaaccgggt gcgcatcccg
     1921 tcgcgcaaga tccttcagga cacctgcgat gcggcttcgg agatcaatgc gaccgccgtg
     1981 atcgtgcacg gcggccatgc cgacgacaac gacatcgagg ccggtttcga gcggtgggta
     2041 aaagcgttgc agtacctcga gaccgatgtg caggtgtacc tggagaacac cgccggcggg
     2101 gaccacgcca tggcccgcca cttcgatgtc atcggtcggc tgtgggatca catcggagac
     2161 agcggcatcg gcttctgcct cgacacctgc cacgcctggg cagcgggcga agcgttgatc
     2221 gatgcggtgg atcgcatcaa ggcgcttacc ggccgcatcg acctggtgca ctgcaacgac
     2281 tctcgcgatg ccgcgggctc tggcgccgat cggcacgcga atttcggcac cgggcagatc
     2341 gaccccgaac tgttggttgc ggtggtcaag gccgccgacg cgccagtgat ctgcgaaacc
     2401 gccgatgagg gccgaaagga cgacatcgcg ttcctgcgcg agcacgtcgg ctgaagcacg
     2461 gttcgataaa cgaacgatta cccaacccgc agtgacgccg cggtgaacta cgctcatttg
     2521 agtgtcggca gtcgacgact cgccaattgc gttggtccgc ctccaggatg cggaccaact
     2581 gcgcgagcgt cgtttgacga ttgcacgtcg catcgccatc gcgacgtggg ccgccgtcat
     2641 cgtctatcga accgtcacaa cgggtttcgc gttcaaccgc gaactgttgc tgctctacat
     2701 ctgcaccgga ctgctggtcg ccagcatcgg ccaaggccgc cgcatgctct acgtcatccg
     2761 cgactggctg ccattcgccg tggtgcttgt cgcatacgac ctgagcaggg gagcggcgac
     2821 gttgatcggg cggccgacga tgtggcactg gcaaatcgac gccgaccgct ggctgttctt
     2881 cggcacggtc ccgacggtgt ggctgcagga gcggctgaag ctgcccttcc cgccatggtg
     2941 ggagatcgtc atcagcaccg tctacatgtc gttcttcatc ctgccctacg ttgtcgccgc
     3001 ggtgctgtgg ctacgcgatc gggaggagtg gaaggcgttc gtccgcttgt ttgtcggtct
     3061 gtcgttcacc gcactgttca tctacgcgct gttgcccgcc gcgccgccgt gggccgccgc
     3121 ccggtgcacg tccacagacg tcgccggcgg cccgtcaggt ccgcgatgca tgttcggccc
     3181 ggcccgaggg gtgcccgacg gcggcctgct cggcgcgatg cagacgacgg cggatggcgc
     3241 caacggctgg gtcgaacgga tcgtcggtcg gggctggggg aagctgaacc ttcagactgc
     3301 gagtgcgctg atcgaccagg gccaggccag cgtgaacctg gtcgcggcga ttccgtcgct
     3361 gcacgccggc ctgaccgctg cggtcgcggc attcctgtgg acgcgagttg ggcgcatatg
     3421 gcggctgctg ttggtggcct acgtcctggt gatggctttc accttggtct acacggccga
     3481 gcactacgtc gtcgacatcc ttctcgggtg ggttctggct gcgatcgtct tggtggtcgt
     3541 ccgtcagtac gaggtgcgtc ggcgaagacg cgctgaaaag gcctacggct gagatcaatt
     3601 ggcagacggc acgaagtcgc cacgcgggcg attcaatggc gcgctgccca ccgcatctaa
     3661 actggcctgg tgctgaccgg ttcgcgtgtc cacccattgc ggccacgcgc attgatcgca
     3721 cagatcgcga tctacgtcgt gatagcgctc gcggcgggcg gttgcttctt cttcgatgtg
     3781 ccatccgaag tgcacatcat cagcgtcggt ggcatgcagc ggacctaccg cctctacacg
     3841 cccccgaatc tcccgccgaa cgctcccctc gtcgtcgtcc tgcacggcgc attggcgaac
     3901 accatcaacg ccgaaacaac ctccggcttc gatcagcggg ccagaatcgg caagttcctc
     3961 gtcgcatatc cagacgaatc gacaagacct ggaacgcgta cggctgctgc ggagacgcga
     4021 cgaaaaagaa catcgacgac gtcgccttcg tcaccgcggt agtcaacgac atccccggtg
     4081 tcgaccgaca gcgcgagtac gccaccggtc tgagcaacgg cgcaatgttg gcccacacga
     4141 tggcgtgccg taccgacctg ttcgcggtaa tcggcgtggt ggcagccacc atgttcgacc
     4201 cgtgcccgga tccgcgtccg acatccgtca tgcacatcca gggcatggct gacaatttgg
     4261 tgaagttcaa cggcaccccc aagggcgata ccgccacggt cgacggtccg ccgatacctg
     4321 acgtgaatgc gttctggcgc agggtggacg attgcgcacc accggaggtc agcaagaacg
     4381 agacggtaac catcacggcg gccaaatgtc ccgataatcg ggaggtcgtg ctggtcacgg
     4441 tcgacgatct cggacacaag tggccggaca atgccgctgc tgcgttctgg aagtttttcg
     4501 agcgccaccc gaagtgatgt gttacgacac ttcttcagct gtcgaaaaaa tgtaatatgg
     4561 gtgccatgcc agacacgcat gtcgtcacca atcaggtccc accgctggac agctacaacc
     4621 cggcaacatc gccggtgctg accgaagcgt tgatccgcga aggcggcgag tggggcctcg
     4681 acgaggtcac cgaactcggg gccctctccg ggagtcgtca ggcccagcgg tggggcgagc
     4741 tcgccgaccg caaccagccg atcctgcgca cccacgaccg ctacggccac cgcatcgacg
     4801 aggtcgaata cgacccggcc taccacgagc tgatgaaggt cgcggtcggc cacgggctgc
     4861 acgccgcgcc gtgggcagac gaccgccccg gcgcgcatgt cgtccgcgcc gccaagacct
     4921 cggtgtggac gcccgagccc ggccatatct gcccgatctc gatgacgtat gcggttgtgc
     4981 ctgcgctgcg caacaacccc gacctggcgg cgatatacga gcctctgctc gccagccggg
     5041 agtacgaccc cgagctgaag gtgccttcca caaaggtggg catcaccgct ggcatgtcga
     5101 tgaccgaaaa gcagggcggc tccgacgtcc gcgctggcac cacgcaagca acgccgaacg
     5161 gcgacggcac ataccgcctc gtcggccaca agtggttcac ctcggcgccg atgtgcgaca
     5221 tcttcctggc gttggcgcag gcacccggcg ggctgagttg cttcttcctg ccgcgaatcc
     5281 tgcccgacgg cagcagaaat cggatgttcc tgcagcggct gaaggacaag ctcggcaacc
     5341 acgccaacgc ctcaagcgag gtcgaatacg acggcgctac cgcatggctg gtcggcgagg
     5401 agggtcgcgg cgtgtcgacc atcatcgaga tggtcaacct gacccggctg gactgcacgc
     5461 tgggcagcgc caccagcatg cgcagcggtc ttacgcgggc catccaccac gcccagcacc
     5521 gaaaggcgtt cggcgcgtat ctgattgatc agccgctgat gcgcaacgta ctcgcggatc
     5581 tcgccgtcga agcggaggcg gcgacgatcg tcgcgatgcg gatggcaggt gccaccgatg
     5641 ctgcggtgcg cggcgacgaa cgcgagacgc tgctgcgccg catcggcctg gcggccagca
     5701 agtactgggt gtgcaagcgc gccaccccac acgccgcgga agcgatggaa tgccttggcg
     5761 gcaacggcta cgtcgaggag tccggcatgc cgcggctgta ccgcgaggcg ccgctgatgg
     5821 gcatctggga aggctccggc aacgtcagcg cgctggacac gttgcgcgcc atggcaactc
     5881 ggccagagtg tgtcgacgtg ctgttcgacg agctggccaa gacgaccggt caagatccgc
     5941 gtctggatgc ccacgtcgat actctcaaac cggcactgga ggacctcgag accatccaat
     6001 accgggcccg caagatcgcc gaggacatca gcctcgcgct acagggctca ctgctgctgc
     6061 gccacggcca tccagccgtc gctgaggcgt tcctggccac ccggatgggc ggtgcgtggg
     6121 gcggcgcgta cggaaccatg ccgaccgggt tggatcttgc gccgatcctg gaacgtgcaa
     6181 tggtcaaggg atgacacacg cgatcaggcc ggtcgacttc gacaacctga agacgatgac
     6241 gtacgaggtg accgatcggg ttgcgcgcat caccttcaac cgccccgaga agggcaacgc
     6301 gatcgtcgcc gacacgccgc tggaactgtc ggcgctcgtc gaacgggccg acctcgaccc
     6361 aggagtgcac gtcattctgg tatccggtcg gggcgaaggc ttttgcgccg gatttgatct
     6421 gtccgcctac gccgagggct cgtcgtctgc tggcggtggc agcccgtaca aggacacggt
     6481 gctctccggc aagacccagg cgatcaacca tctgcccgat cagccgtggg acccgatgat
     6541 cgactaccag atgatgagcc ggttcgtccg cggcttctcc agcctcatgc acgccgacaa
     6601 gccgacggtc gtcaagatcc acggctactg cgtggcgggc ggcacagaca tcgccctgca
     6661 cgccgatcag gtgatcgcgg ccgccgacgc caagatcggc tacccgccca cccgcgtgtg
     6721 gggcgtgccc gccgcaggca tgtgggcgca tcggctcggc gatcaacgcg ccaaacgcct
     6781 tctgctgacc ggtgattgca tcaccggagc gcaagctgcc gaatggggcc tcgcggtcga
     6841 ggcacccgaa cccaaggatc tcgatgagcg cacggagcgc ctcgtcgagc ggatcgcagc
     6901 gaacccagtg aaccagctca tcatggtcaa gctggcgatg aacagcgcgc tctacaacca
     6961 gggagtggcc aacagcgcga tgatctcgac cgtgttcgac ggcatcgccc ggcacacgcc
     7021 cgaaggccat gcgttcgtcg cccagtctcg cgagcatgga tttcgggaag cggtccgcca
     7081 gcgcgacgag ccgttcggcg attatggccg caaggcgtcc ggggtgtaac ggtgccgaca
     7141 cttgcgcgga tgacggcccg ctccgtcgtg ctgagcgtgc tgctcggcgc ccacccggcg
     7201 tgggccaccg cgagcgaact gatcaggctg acagccgatt tcgaccttag ggagccgacg
     7261 gtgcgggtgg cgctgacccg gatggtgagc gcgggtgacc tggtgcgctc cgaggacggc
     7321 taccggctct ccgaccggct gctggcccgg cagcgccgcc aggacgacgc gctcaatccg
     7381 caggtgcgca agtggaacga cagctggatg acgctggtga tcaccagcgt cggcattgat
     7441 gcgcgtaccc gcgcggcgtt gcgaaccact ctgcaggaca aacggttcgc cgaactccgc
     7501 gagggcgtgt ggctgcgacc cgacaacctg gacatggacc tgacgcccga tgtgctcgac
     7561 cgggtgcggg tactgcgctc ccgcgacgac gactcttcag agctcgccgc ccggctgtgg
     7621 gacctgccgg gttgggtgcg gaccggccac cacctgctcg acgaaattgc aacggccact
     7681 gacattcccg gccgattcgt cgccgcggcc gccatcgtgc gccacctgct gaccgacccg
     7741 gtgctgcccg acgaactgct gcccgacgac tggccaggcg atgcgttgcg gacggcctac
     7801 acgaacttcg ccgccgaact ggtcgcgcgg cgcgacggtg tcgaactaat ggaggcaacg
     7861 tgacgagtgg tggagtgcgg gtcgagagca acggcccggt gacgaccgtg atcatgaatc
     7921 gacccgaggc gcgcaacgcc gtcaacgggg ccgccgccgc ggagttgtac gaagcgttcg
     7981 acgagttcga caaggacgat tcggcatccg tcgcggtgct gtggggtgat aacggaacat
     8041 tctgtgcggg agccgatctc aaggcgttgg gcacagccga cgccaacccg gtgcatcgca
     8101 ccgggcccgg tccgatgggc ccgagccgga tggtgttgtc caaaccggtg attgccgccg
     8161 tcagtggcta cgcggtcgcg ggcggcctgg agttggcgct gtggtgcgac atgcgcgtcg
     8221 tcgaggagga cgcgatcatg ggcgtcttct gccggcggtg gggcgtaccg ctgatcgacg
     8281 gcggcacggt gcggctaccg cggatcgtcg ggcacagccg cgcgatggac atgatcctga
     8341 ccggccgcgg cgtcgacgcc aacgaggcct acgcgatggg gctggccaat cgcgtcgtgc
     8401 ccaaagggca agcgcggcaa cgcgccgagg aactcgccgc ggaactcgcc cagctgccgc
     8461 agcagtgcat gcggtcagac cggctgtcga tgctcaacca gtggggatca tcagaagcgg
     8521 aggcgatgga cttcgaattc gccagcattt cgcgcgtcgc gggcgaatca cttgagggcg
     8581 cagcacgatt cgccgccggc gccggccggc atggcgcgag cgcctagccc ttgctgatct
     8641 tgttgttcga cccggtgtcg ttgatcttcg ggtcgccgtc cttgtaggtg acggtgttgt
     8701 tcaggccgac gacgctaatt tctttgtcga ccttctcgaa ggtgaccttg ttgtcggcgc
     8761 cgccgatgtt cacgttggcg cacgtgccct tcacggtcag ggtgttgttg gacccgccga
     8821 tgttgagcga ctttccatcg gcgcaatcga tatccgccgt ggtgccgaac gagccgtaat
     8881 tgatcgtgtt gccgatctcg acctgcgcac ccgacgaacc agccgtcgcc gtcggtgtgg
     8941 tggtctcctt gctttccgaa ccgcaaccgg cgaggactag tgcggcgagg gccaccgctg
     9001 cgaaaagccg aatgctcacg cggccacccg atcaacgcgg ttgaccatgc ccaattcccg
     9061 gccgcggtcc cacacgaccg gctcgccact cttgtagaac accgtctggt cgcttccgta
     9121 cacgatcacg ttgtcgacga tgttgtcggc gacgacgacg ttcgacgaac cctgcacagt
     9181 caccgcccag cagctgccca gtgcgtagac cgtgttgccg gttcccaaga caaacaacgt
     9241 cgactcgttg cagtcgatcg tctgcgtgat gccgttcccg gtgatgtggg tgtcgccgtt
     9301 cgccgcttga gccgacggtg ctccgaggcc gaacgcgacc gggaacgcga cggcgcacgc
     9361 cgccagcgat cgacccacag ccttcgatgt catgcaggga gcgtacgtcg ctgcgcggcc
     9421 atcggggcag tggttcgcct gagtcgcctt cgttgatcgt gctgggtagc gtgttgagcg
     9481 atgcgttggc gggtcaaccc ggttgctgtc gtggcggtca cagcggtagt ggccgtcgca
     9541 gggtgttctc agaccgtcag tggaaccgcg cgtcgctcgc agcccggcgt gcccgatccc
     9601 aaccgcagct atggctacgt cgacgatcgc tgcgggttgt tggtcgacgg ctcgattcag
     9661 cagacgatcg gcgccgacca catcgttcgg ccctacagtg gcgcggtctg ccagtacgta
     9721 ttggcgcgcg gttcggccat ggtcgacgtc actttctcct ggtatgagac cggcaatctg
     9781 gaccgcgaac gtcggctcgc cgcgcagcgc ggcgcgacga tcaccgacaa gatcgtcgaa
     9841 cggcatcagg cgtttctggc aagtcgtgac accaccggcg cggcctgttc agccaccgcg
     9901 gcggcaggca ccggggtgct cagttggtgg gtgcaattcc gcggccaaca caacggcgat
     9961 ccgtgtaagg acgccgagaa gctgctcacc gcgactctgc agtcggacat gtgacgatgg
    10021 ccctgctgag atcgacgtgc tgggcggcgc ttgccgtagt cgcggcgctg cttgttggct
    10081 gcggcccgtc cgagaaccag tcggcaccgc ccgccactcc gtccgcgcca cctgacgctg
    10141 gcttccgcag cgcagactgc aacggggtca ccgatgccga cgtcaccaag gccgtcggat
    10201 cgtcgatgtt caccaaggcg gtcgtcagcg atgccggatg cttctggcag gaaaacacgg
    10261 tgctgggcac cttcggccag ggcatgggta tctcgacctg gtggtatcgc ggcagcgata
    10321 tggacaccga acggtcgctg gagcagcagg ctgggcgcac actcaccgaa ttgacgattg
    10381 acggcaacaa gggtttcaag gcttacgacg ccaacgcgtg cagcatctat gtggccaagg
    10441 gcaccgacgt catcacctgg tcgatccaga cgatgaatcc agcaatgctg cccgatctgt
    10501 gcacaatcac cgggcaactc gccgagctca gtcaggagcg cgtcaactga ccgctgggaa
    10561 actagaggtg ttagttttcg ctgatcacgt tcgagattcg acgaagggtg gccggcacat
    10621 ggcagctccg cagccagcgg gcgcccgacg atccacgagg ttgagccgcg attccatcgt
    10681 caacgccgcg ctgaccttcc tggatcgtga gggttgggac gcgctgacca tcaacgcgct
    10741 ggccacccag ctcgggacca agggcccgtc gctgtacaac cacgtccaca gcctcgatga
    10801 cctacgccgc accgtgcgga tgcgggtcgt tggcgacatc atcgacatgc tcaacaccgt
    10861 cggtcagggc cgcacccgtg atgacgcggt gaccgctatg gccgccgcct accgcagcta
    10921 cgcccatcac catcccggcc ggtattcggc gttcacccgg atgccgctcg gtggcgatga
    10981 ccccgagttc accgaggcga cccgggcggc ggccgccccc gtcatctcgg tactggcctc
    11041 ctacgggctc gacggagaga gtgcgttcta cgccgcgctg gagttctggt ccgcgatgca
    11101 cggtttcgtg ctgctggaga tgaccggggc gatgaacggg atcgacaccg acgcggtgtt
    11161 caccgacatg gtgatgaggc tggcgtccgg gatggagagg cggtagcgct gacctgcgaa
    11221 taggcggtcc gggatgggtt tggagcggtg ccccagccct ggtattgtgg acgatcgtgc
    11281 ctggcagata aggcgcttgt agacgtgaaa agctcaagca tgcctgcacg ccgggccaat
    11341 tcgcgtgtcc acgaagccta gagaaaatct acgcggcggg cgcatgcgac acacccgatc
    11401 gcggggtacg tgaacgggac ataactgcag tacagagact tgaaaaacga agtagcaaca
    11461 cagaaagccg gtagatgcca accattcagc agctcgtccg caagggccgc cgcgacaaga
    11521 tcgccaaggt gaagaccgcg gccctcaagg gcagcccgca gcgtcgcggc gtgtgcaccc
    11581 gcgtgtacac caccaccccg aagaagccga actcggcact tcgcaaggtt gcgcgcgtca
    11641 agctgacgag ccaggttgag gtcaccgcct acatcccggg cgagggccac aacctgcagg
    11701 agcactcgat ggtgctggtg cgtggcggtc gtgtgaagga cctgccgggt gtgcgctaca
    11761 agatcatccg cggctcgctc gacacccagg gtgtgaagaa ccgcaagcaa gcccgcagcc
    11821 gctacggcgc caagaaggag aagagctgat gccgcgcaag gggcccgcac ccaagcgtcc
    11881 gctggtcaac gacccggtct acgggtcgca gctggtcacc cagctggtca acaaagttct
    11941 tctgcaaggg aagaaatcgc tggccgaacg cattgtttat ggtgcgctcg aacaggctcg
    12001 tgacaagacc ggcaccgatc cggtcgtcac cctcaagcgc gctctcgaca acgtcaagcc
    12061 cgcactcgag gtccgcagcc gccgcgtcgg cggtgcgacc taccaggttc ccgtcgaggt
    12121 gcgcgccgac cgttccacca cgctggcgtt gcgctggctg gtcagcttct cgcggcagcg
    12181 ccgggagaag accatggtcg agcgtctggc gaacgagatc ctcgacgcca gcaatggcct
    12241 gggcgcctcc gtcaagcggc gtgaggacac ccacaagatg gccgaggcaa accgcgcctt
    12301 cgcgcactac cgctggtgac ctagtcgccc gtcgtgccgg agccgcgcag gcggcgacgt
    12361 cgggcaccgt ggcccggacc gcacaactaa agcaactatc aagcgaaaga gatgacttct
    12421 gtggcacaga aggacgtgct taccgacctg agcaaggtcc gcaacttcgg catcatggcg
    12481 cacatcgatg ccggcaagac gacgacgacc gagcgcatcc tctactacac cggtatcaac
    12541 tacaagatcg gtgaggtgca cgacggcgcc gccacgatgg actggatgga gcaggagcag
    12601 gagcggggta tcaccatcac ctcggctgcc accaccacgt tctggaaaga caaccagctc
    12661 aacatcattg acaccccagg gcacgtcgac ttcaccgtcg aggtggagcg caacctgcgc
    12721 gtgctcgacg gtgccgtcgc cgtgttcgac ggtaaggaag gcgttgagcc gcagtccgaa
    12781 caggtttggc ggcaggccga caagtacgac gtgccgcgca tctgcttcgt caacaagatg
    12841 gacaaggtcg gcgctgactt ctacttctcc gtacgcacca tggaggagcg acttggcgcc
    12901 aacgcggttc cgatccagct gccgatcggc gcggagggtg acttcgaggg catcgtcgac
    12961 ttggtcgaga tgaacgccaa ggtgtggcgc ggcgagacca agctcggcga gacctacgag
    13021 accatcgaga ttcccgcgga cctcgccgag caggccgacg agtaccgcac gaagctgctc
    13081 gaggtcgtcg ccgagaccga cgaggcgctg ctggacaaat acgtcggcgg cgaggaactc
    13141 acggtcgagg agatcaaggg cgcgatccgc aagctgacga tcaacagcga gatctacccg
    13201 gtgctgtgcg gcagtgcatt caagaacaag ggtgtgcagc cgatgctgga cgcggtcgtc
    13261 gactacctgc cgtcgccgct cgacgtgccg ccggccgtcg gccacttgcc tggcaaggag
    13321 gacgaggaaa tcgtccgcaa cgcgactgcc gatgagccgt tcgcggcgct ggcgttcaag
    13381 atcgcgacgc acccgttctt cggcaagctg acctacatcc gcgtgtactc cggcaccgtc
    13441 gagtccggct cgcaggtcat caacgccacc aagggcaaga aggagcggct gggcaagctg
    13501 ttccagatgc actccaacaa ggagaacccg gtcgaccggg cctccgcggg gcacatctac
    13561 gcggtgatcg gcctgaagga caccaccacc ggtgacaccc tctccgatcc caaccagcag
    13621 gtcgtgctgg agtcgatgac gttccccgat cccgttatcg aggtggccat cgagcccaag
    13681 accaagagcg accaggaaaa gctcggaacc gcgatccaga agctcgccga ggaggatccg
    13741 accttcaagg tgcacctgga tcaggaaacc ggccagaccg tcatcggcgg aatgggcgag
    13801 ctgcacctcg acatcctggt cgaccggatg aagcgcgagt tcaaggtcga ggccaacgtc
    13861 ggcaagccgc aggtggccta caaggagacc atcaagcgca tggtcgacaa ggtcgagtac
    13921 acccacaaga agcagacggg tggctccggc cagttcgcga aggtgctcat ctccattgag
    13981 ccgttcaccg gcgaggacgg cgccacttac gagttcgaga acaaggtcac cggtggccgc
    14041 atcccgcgcg agtacatccc gtcggtggat gccggtgcac aggacgccat gcagtacggc
    14101 gtgctcgccg gctacccgct ggtgaacctg aaggtcacgt tgcttgacgg ccagtaccac
    14161 gaggttgact cctcggaaat ggcattcaag gtcgccggct cgcaggcgct gaaaaaggct
    14221 gcgggacaag cacaaccggt gatcctggag ccgatcatgg ccgtcgaggt tacgacgccc
    14281 gaggactaca tgggcgatgt gatcggcgac ctgaactccc gccgtggtca gatccaggcc
    14341 atggaggagc gggcgggtgc acgcgtcgtc aaggcgcatg tgccgctgtc ggagatgttc
    14401 ggctacgtcg gagaccttcg gtcgaagacc cagggccggg cgaactactc catggttttc
    14461 gattcgtacg ccgaggttcc ggcgaacgtg tcgaaggaga tcatcgctaa ggcaaccggg
    14521 caatagcccc ggttgctttg cggggacgaa caactgcaaa gatcaacact gctttacaac
    14581 aagcaccaac acagtccagg aggacacaac agtggcgaag gcgaagttcg agcggacgaa
    14641 gccgcacgtc aacatcggga ccattggtca cgttgaccac ggcaagacga cgctgaccgc
    14701 agcaatcacc aaggttctgc acgacaagta cccggagttg aacgagtcgc gcgcattcga
    14761 ccagatcgac aatgcgcctg aggagcgtca gcgcggcatc acgatcaaca tctcccacgt
    14821 ggagtaccag accgagaagc gtcactacgc gcacgtcgac gcccccggtc acgccgacta
    14881 catcaagaat atgatcaccg gtgccgccca gatggacggt gcgatcttgg tagtcgccgc
    14941 gaccgatggc ccgatgccgc agacccgtga gcacgtgctg ctggcccgcc aggtgggcgt
    15001 gccctacatc ctggttgcgc tgaacaaggc cgacgcggtc gacgacgagg agctgctcga
    15061 gctcgtcgag ctggaggttc gcgagctgct ggccgcccag gagttcgacg aggacgcccc
    15121 ggtgatccgg gtctcggcgc tgaaggcgct cgagggcgat gccaagtggg tcgagagcat
    15181 cgaagagctg atgtcggctg ttgacgagtc gatccctgac ccggtccgtg agaccgacaa
    15241 gccgttcctg atgcccgtcg aggacgtctt cacgatcacc ggccgcggca ctgtggtcac
    15301 cggtcgtgtg gagcgcggcg tggtcaacgt gaacgaggaa gtcgagatcg tcggcatccg
    15361 tccggacacc accaagacca cggtcaccgg cgtcgagatg ttccgcaagc tgctcgacca
    15421 gggccaggcc ggcgacaacg tcggtctgct gctccgcggc atcaagcgtg aggacgtgga
    15481 gcgcggtcag gtcgtcgtga agcccggcac caccacgccg cacaccgagt tcgagggcca
    15541 ggtctacatc ctgtccaagg acgagggcgg ccggcacacg ccgttcttca acaactaccg
    15601 tccgcagttc tacttccgca ccaccgacgt gaccggtgtg gtgacgctgc cggagggcac
    15661 cgagatggtc atgccaggtg acaacaccga catctcggtg aagctgatcc agcccgtcgc
    15721 catggacgag ggcctgcgct tcgcgattcg cgagggcggc cgtaccgtcg gcgccggccg
    15781 ggttaccaag atcatcaagt gatctgatct agtaccgcaa agcggcgctc accttcgggt
    15841 gggcgccgct ttcgtgtatg cacgcgatcc gctcagcgat gagcggacag gtgcagcgcg
    15901 catctgccgt gcgtcagagt taactagaag cacgagaatt ctgatcaagc ttggttctga
    15961 atttgctgtc acgttagttt gctcacgcaa taattgtcac tcccgtaaca acatcatccc
    16021 gctgtcattc gccaccaata atcgaaggtc agatgccata tttgggtagc cacacacatc
    16081 gtcagaagcc cgacatctgg gcatggaact tcgccatagc tgattgtcaa ggttcgtttc
    16141 caacgcaatg tgacgcttga cacaccagat agcaaagttt taacgtcagc gtcagcaact
    16201 tagggcttcc agttcgggta cgcgcgcagg aggccgcgca agcggtcagc cctcacccgc
    16261 acgatcgcag cgggtggcgc ggttgtctgt atcgccgcgg cctacgaggt caccgccccg
    16321 accgtccacg ccttgtcgat cgtgtttcgc tcgccggacg gaaacggcgg tacgaacgag
    16381 acccggatca atgtcctgca cggcaacatc ttcgatccgc agctggggct cctcggcgcg
    16441 aacgtcagta acaactcgac cactcccaac ggcacggcca gcaccgccaa caacccactc
    16501 gtcaacaact tgatcttcaa caacccgatc acgcagttcc tgaactcgct gttgaacgcg
    16561 gagatcgtgt tcggcggagc ggctgcgggt cccatcaaca actcgacgca gatcagcctc
    16621 ttctcgttca acatcttcaa tccgcaggcg agctttttcg gtcccaacgt cggcaataac
    16681 accgctatca caaactacgc ggagaacaat gggaactaca gccagaccag cgtgaccagc
    16741 gggggtctct taggtgcggt tgtacgtttg ttcctcggtg gtggaatgac cggcaacgga
    16801 aatacaactc aaattgcggg cggcacctcc aacataacca atccgcaagt cagtattggc
    16861 ggctcgaacg taagcaacaa tacagcggtc acgaacgaag cgatcggtaa cggcaatagc
    16921 agccaaacca cagttggtgg gtcaggaaac gtcatatcgg ttggtacgac tggtaacggc
    16981 aacacgaacc aaatcgccgt cggtaccagc aacatcgaaa acacgcaggt gagtattggg
    17041 gatgtcgggc tgagtcctcg tccggaggaa ctcagtgcta accagtccgg cactcagctc
    17101 gccaccacca gcacgacccc aacttcgagt tcggtgagct ctaccgattc gcagagttcg
    17161 agttcaatcg gcactgccag tctcaccgac acctctggat cgtcggcgcc tgaaagcggc
    17221 agcgttacaa gcggttcggg tggcacaact ggcacgggcg gcacaaccgg tactagcggg
    17281 tcgggcggga caagcggttc tggcggttca ggagattccg gcggttccgg tggctcaagc
    17341 ggttcaggcg gtgaatgagg tgaacaggag gtgcgaatga ggtgaacgta accggcagtg
    17401 tcgtcgctga gtgtcgcact gcatcacatt cgacaaccaa acaacgtcga cacaagaagg
    17461 acttcagaaa tgagtaagca tcgcaaggca cgcaaagcgc cgatgccagt gaagctcaat
    17521 cgcgtcattg ctatcggcgc cgtcgccggc agcactgtcg gattcggggc actcttggca
    17581 gcgaacccac caatggctta tgccgacgaa tccagttggt ggttgttgag cggtaataac
    17641 actccaattg cgagcgacaa caatattgtt atcggcacca atggaaatgg caacacgaac
    17701 caactcgcct tcggtacagg cattattgga aacgcgcaaa tcaacctgtt cagcctcggc
    17761 gtcgccacgg tggccaacgc cgcccccgcg gttggtggca acgcgatcgg cggccccgcg
    17821 ctcagtacgg ccaccgaagc aggtgtgccg atcggcgggc tggcagtcgg aggcgcggcg
    17881 gtcaacccgg cggtcggcat caacccggcg ctgggcggca taggaggcct cggcacggcg
    17941 ctcggcggtc cggcgatacc ggtggcgttg ggtggcctgg caacgcaagt ggcgaccggc
    18001 acccaagcga acaccgcccc tggcggagac gcaacgggcg gctcggccac aaacgccggc
    18061 aaagcgacaa gcgggcgcgc gaccggcggc accgccatcg gcggcaacgc gaccaccagt
    18121 gcttccggcg tcggatctgg caatttgtcg aataacaact cgagcacaaa cacctcgacc
    18181 ggcaacggca actccagcag cagcagcaat ggcggaacca atgacggcag cagcagcggc
    18241 ggcaccagca gcagcggcgg ctgaccgaat gtaagtaggc aaccccggcc cgcgctggac
    18301 cgccgtttcc gctgttcacc ggcgtaacac gtgagacggc tgaggcgatg tatatcgcga
    18361 atgccctggt cggggcagaa gaagggttgg ggtttcatgt ttcgacgcat cactacgatc
    18421 gcctgcaccg ccgcgatggc cggcgctgcg ttcgcagggc tgtcggccgg taccgcatct
    18481 gcggtccccg gctgcccgga cgtccattgg atcggcgccg caggctcggg cgagcgcacg
    18541 cccgcagagg tgactcagaa cggcggaatg ggccgcgtgg tctaccagtc tctgcaggag
    18601 ctttccgccg aggcgcagaa ggacggccgc acgatgacag ccgaggcggt cgagtacccc
    18661 gccgtggaag cgcccggtga ggatggcgga atcggcgagt ggctcggctt catgggcagc
    18721 gtcgacaccg gcgcagccgc gcttagcagg cagtacgagg cgttcgtgca gcagtgcccg
    18781 acctcaaagg tcgtgctggc cggctactct cagggcgcca tggtggtgca ccgcaacctc
    18841 gctgcactcg aagcaagccc gaatctggcc gccgcgctgc tggtcgccga cggcgatcgg
    18901 ctgcccgcag acccgaccct caacttcggc acggccaatg gcatcccgga gcgcggaaag
    18961 ggtgtcgcgc aggactggcc gatcctggcc cacgcgcccg ctccgctgtc gcccgccatg
    19021 ggcgctcgga cgatcagcgt gtgcgatctc aatgatgccg tctgcgacta cgacgaggac
    19081 gccgacgact ccacaggggc gtacgcgcgg cgcgtggtcg tccacaccag ttacgcgaga
    19141 accccgggat tggcctggac cgcgccactg cacttcctac tcggaccggc gcctgcgtcg
    19201 cccgaggtcc acgaagtcac cccgctcagc gcgactgcgc cgactcagta gccgcccgcg
    19261 cgatcaacag caccactaca ccggcgggca gcagtagcgc cgccagcacc gcgacgatgg
    19321 tgtcgaccgc accggcagtc gacattccgg cgcgctgaag catctccagc accgttgcgg
    19381 acacggcgac gccgacgccg gcaagcgtga ccaacgcggt cagcgtgacg cccgcagcct
    19441 cgcccgcccg ttcggtgcgc acgaccgcct gagtcgcgac cgtcgtgaac gcgtacacca
    19501 atcccagcgt taatccacac gcggtgagtg ccggcaggta gacgacccag gtgtgcgatg
    19561 acgcgagcgc cgccaacgac atcgcggccg cgacacttgt cagacccatc acgagcactg
    19621 gcgggcgact ggccgcgagc cggcctgcca gcacgccgcc aagtgcggca cccgccgacg
    19681 gtccgaggaa taccaggccg gcggtcaacg ggtcgaggcc gcgcacctgt tgcagataca
    19741 tcgtcgacag gaagatcgtc acgccgtagg cgatgttggc aatggtgccc gcgatgacca
    19801 ggatcgtgaa tctggggttt cgcattagcg acagatccac caacggccag cgcacccggt
    19861 tctccgctac cacgaaaaaa gcgagtgccg ccagagagca cacgaacgcg atgaccgtcg
    19921 gcgccgacag ccaaccccag gacggtgctc gatcaaatgt caacgtgaac aagccaattc
    19981 cgacggtgat gagggccaag ccggtgacgt cgatccgacg cggcaccgtc tcgtcgaagg
    20041 attcggtgat gctccacgcc ccgatggcaa gcgcgatcag tgtcagcgga acgttcagcc
    20101 agaatatcca ccgccagccg accgtctcag tcagcagccc accgatcaat gggcctgccg
    20161 cgttgcccaa tcccgctatg ccgtaagcca acccgattgc atgactggcg cgcacagacg
    20221 gaaaggcgtt tgtcagcacg ctcacagaaa ccggaaagat cagcgccgcg cctatgccct
    20281 gaacggcgcg gaaggcgacg aggagcgtcg ccgatggcgc cagcgcacac agcgccgagg
    20341 acaagccgaa taacacgatg cccgccaaga gcgcgcgtcg tcggccgaag atatcgccga
    20401 tccggccggc cggcaccatg aatgagccca gcgccagcat gtacacgctg atcacccatt
    20461 gcaggtccgc cgcggtgctg ttgaggtcaa atgccatccg cggcaacgcg agattcatgg
    20521 cgaagtagtc gatttgcacg cagaacagcg ccatcgacac ggccgcaagg gtccaccgga
    20581 gacgcacagg ttcgtccgtc gcggtcaccg cacgacgata cgtctcgcaa ccgccggttt
    20641 ggcgctaatc tgacacgcga accgggcaaa cgacaggagc gtggcagatg ttagggcggt
    20701 acatcaagtc tcagatcatg gtcctcgtct gtggcggcct tgtcggtccc atctttttgg
    20761 gggtctactt cgcaacaggc cagagcggca ttctgaagtg gatgttctgg gtcggccttc
    20821 tcatcaccgc tgccgacgtg ctgatcgcgc tggcgctggc gaacttcggc gcaaagtcgg
    20881 aagccaagac ccaggcgctg gaacagagcg gcgtcttggc gctggcgcag gtggttggca
    20941 tacaggagac gggcacccgt atcaacgagc agccgctggt gaaactcgac ctgcagatct
    21001 ccggccccgg catcacccca ttcgccacgc aggaccgggt catcgcatcg atcacgcggc
    21061 tgcccatgat caccaaccgc aaacttgtcg cgctagtcga tcctgccaca aatgagtacc
    21121 aaatcgactg ggagcgaagt gctttggtgg gaggcgtgat gccggccaac ttcaccatcg
    21181 ctgaggacaa caagacctat gacctcagcg ggcaggccgg cccgctgatg gagatcctgc
    21241 agattctcaa ggccaacggg atcggcatga acaacatgat cgatctacgc tccaacccgg
    21301 cggcccgtgc gcaggtgcag gccgtggtca ggcgcgccgc ggccgcgcag caggctccgg
    21361 cagccgcggc accggcggcc gcccccgccg cggcacccgg tgcgccgtat gcggcgacgc
    21421 ccgagccgag caccgcgcag cggctgcaag aattggaaac attgcgcgct accggtgtga
    21481 tctctgacga cgagtacaac gccaagcgtc agcagatcct ctctgacctc tagctggatt
    21541 cgtgaatccc cgatgcggct gatgtgctgt cgcataggct tcttgcgact gtgagtgaat
    21601 gcatcggtgg ttcatgacgg caacggagca gcctgaaaag gtaaccgaaa aggtcagcct
    21661 cccaacactg accgcgatgg ttgtcggctc gatgatcggc tctggcgtct tcctgctgcc
    21721 gcgccgcttc ggcaccgaga ccggcgtgct tggggcgatc atcgcgtgga ccatcgcggg
    21781 ctccggtatg ttgatgctgg ccttcgtctt tcagcgtctc gcgacccgca agccggatct
    21841 ggacgcgggg atcttcgcgt atgccaaggc cggtttcggc gactacgtag ggttcaattc
    21901 ggcgatcggg tactgggcat ccgcctgcgc gggtaacacg tcgtactggg tgctcattac
    21961 ggcgacgatg agcgcggtgg tgccgggatt cggcgacggc gacacggtgc tcgcggtggt
    22021 tgtgtccgcg attgctgtct ggctgttcgt ctacctcgtc ctgcgtggtg tcaaggacgc
    22081 agccgtcatc aactacatcg tcacgatcgc caaagttgtg ccgattctga tgtttatcgt
    22141 gatcactgcc atcgcgttca aggccgacgt gtttgccgac aacttctggg gcggcgacga
    22201 ctactcgttc tcgtcgatct gggaccaggc caccggcacg atgctgatca ccgtgttcgt
    22261 cttcctgggc atcgagggcg cgagcgttta ctcgcggatg gcacgtaagc gggaagatgt
    22321 cggacgagcc accatcatcg gattcctcag cgtgctcagc gttttcgcgc tggtgaccct
    22381 cgtgtcgtac ggcgtcatgc cacagagcga actcgccggc gtcagccagc catccatggc
    22441 ggcggtgctt gaatccgttg tcgggcattg gggatcgatc ttcatccgca tcggtgtgat
    22501 cgtgtccgtg ctcggcgcct atctggcttg gaccctgatg gccgccgaga tcctctacat
    22561 ccccgccaaa accgacgaca tgccgcgctt cctggcccga accaacagcc atggcgcgcc
    22621 cgtcaccgcg ttgatcatgg ccgccggcct gatttcactg ctgcttatcg cgctgctctt
    22681 cgccgcggac gcgctcgact tcatgctgga cctgaccgcc gcgctgtccc tgattcccta
    22741 tctcctcgcg gcggcctatg cgttgaagtt gaccgccact cgcgagacgt acggcgacgg
    22801 caaatcgctt gttccagaca tggtcatcgc cgcggtggcg acggtgtaca ccttgttcct
    22861 gatctacgcc gcgggtgtcg acaagcttct cctgtcctgc atcttgtacg cgccggccgc
    22921 cgcgctctac ttcaaggcgc gccgcgaacg cggccttcgc gtgttcaaac cggcggaggc
    22981 ggtgctgttc ggcgtgattg tcctcggtgc cgtcgccggt gtggtatcgc tggcaactgg
    23041 agccatcgaa atctagccgc cactgggcga aaggagccga cgatgaccaa cccgtccacc
    23101 aattacggcg tgcactctga agtcgggaag ctgcgcaagg tgttggtctg ctcgcccgga
    23161 ctggcgcatg agcggctcac gccgaccaat tgcgacgacc tgttgttcga cgacgtgctc
    23221 tgggtgcaga acgcccgtcg tgaccacttc gacttcatcg acaagatgcg agaccgcgat
    23281 gtcgaggtgg tcgaactgca cgacgtgctc accgagacga tgaaagatcc ggccgcaaag
    23341 gcctggctgc tggaccgcaa gatcgtcccg aacgaggtgg gcctcggcct tgtcgacgac
    23401 acccgcggct acctcgactc gttgcaaccg cgccggctcg ccgagttgct gatcggcggc
    23461 atgtcgacgc gcgacctgcc caaggaattg cagtcgggct accgggcgct ggcacgcgaa
    23521 agtactggcg tcacagagta tttgatgccg ccgctgccga atacgctgta cacccgcgac
    23581 accacctgct gggtctatgg cggggtgacg ctgaacccgt tgttctggcc ggcgcgacac
    23641 gacgagacac tgctgatgaa ggcgatctac gagttccatc ccgacttcgt tggctccacg
    23701 gtgtggtggg gcgatcccga ggaacagtgg gggatggcga ctttcgaggg cggcgacgtc
    23761 ttggtgcccg gcaatggcgt cgtgatcatc gggatgagcg aacgcacgtc gcggcaggct
    23821 atcacccagg tggcgtccag gctgtttgcc gaggaggctg cgacccaggt gatcgtcgcg
    23881 ggcatgccca aactgcgggc ggcgatgcac ctcgacacca tcttcacctt cgccgaccgc
    23941 gacgtggtca ccatctatcc cgacatcgtc gacgccatgc agaccttcac gctgcatccc
    24001 agtgacaacg acgtcggggt ggaggtcgtc gaggccgaca agccgttcgt cgaagtggtg
    24061 gctgccggtc tcggactggg caagctgcgg gtggtcgaaa cgggcggcac cgcctacgac
    24121 tctgagcgcc agcagtggga cagcggcaac aacctcgtcg ccgtcgaacc cggagtggtg
    24181 ttcgcctacg accgcaacac ccacaccaac acactgctgc gccgtgccgg catcgaagtg
    24241 atcaccatcg tcggcgccga gctcggccgt ggtcgcggcg gaggccactg catgacctgt
    24301 ccgctggccc gcgactccgt ctactgagag gcacggcgac tagaacacgt ttcagttctg
    24361 ctgttagcct ggttcgcagg ctcacgaaag gagcagcggt gacggcaacc ggcggaccac
    24421 tggaaggacg agtggcgttc ataaccgggg ccgcgcgcgg ccaagggcgg gcgcacgcga
    24481 tccggctggc cagcgagggc gccgacatca tcgcgatcga tatctgcggc ccgatctcgg
    24541 agaccatcac ctacccgctg ggcacctctg aggagttggc cgagacggtt gctgcggtgg
    24601 aagccaccgg ccgcaaggtg ctggcccgcg aggtcgacat ccgcgacctg ccggcgctgc
    24661 aacaagtggt ggccgacggc gtcgagcagt tcggccggct ggacattgtc gtggccaacg
    24721 ccggggtgct gagctggggc cggatgttcg agatgtccga agagcagtgg gacaccgtca
    24781 tcgacgtcaa cttgaacggg acgtggcgga ccatccgcgc tgcagtgccc gcgatgatcg
    24841 aagcaggaaa tggcgggtcg atcatcatcg tcagttcatc ggcaggaacg aaggccacgc
    24901 ccggcaacgg gcactactcg gcgtccaagc acgggctggt cgccatcacc aacgcgttgg
    24961 cgatcgaggt gggtgagttc gggattcggg tcaactcgat tcacccgtac tcgatcgata
    25021 cgccgatggt ggagaaggaa gcgatgatgg ccgtcttcgc caagtatccg cagtatctgc
    25081 acagcttttc gccgatgccg taccgccccg tcaaccacgt tggcaagaag ggcctgcagg
    25141 aattcatgac ggccgaagag gtttcggacg tcgtggcctg gttggccggt gacgggtcgg
    25201 cgacgatctc gggcagtcag atcgccgtcg accgcggcac cgctaagtac tagctagccg
    25261 ggcagcacca ggactggaac cgggctatgc cgcaagatct ttgatccgcg cgagccgagg
    25321 aacacgcgcg cgattccgcc cacaggcgat gtgccgatgg ccagcaggtc gccctcctgc
    25381 cagtcggctt catccagtgc ctcgtcccag ccattacccg tgacgacctg cagctccacg
    25441 tctttgccga tgacgccgtc ggtcttcaac tgcgccagcg tttctcgcgc atgcgctgcc
    25501 aactggtcca ggatcgagtc ctccgtgctc agcccgactt cgggcgggta catcgtgcgg
    25561 ccgcgaacgg cgaacgtgac gacccgcatg gggacgctga gccgctccgc aagcgccgca
    25621 acccgttcga cgacatgaag cgcctccgga gtgccggggt aggcggctgt gatccgcgtc
    25681 agtccacccg ctttcgaacc gcgatagccg cgtgggctga tcgccatagg aaccggcgag
    25741 gagtgcaaca gccggtcggc ggtcgatccg accaccacct ggccgagctt gccgtcggac
    25801 gcggagccca agacgagcac ctcgggttcg agttcggaga ccgcctccaa caagccaccg
    25861 gttgtcgagc ggtgggcgac cttgtggtac ttgacgtcga gcccctgacc gatcgagtcg
    25921 atgcacccct gcgcttcccg ggccgagttc gccgccaatt gctcagcgta ctgggcatat
    25981 tcggcgtcga tccggcccag cgacggcgtc atccacggcc gcggcacgac ggtgaccacc
    26041 gtcaacgacg ttttgagcgt cttggccgcc tcgaccgcca ggtacagcgg cgaccggccg
    26101 cccttgccgg ccaggtagcc gacggcgacg gtcacggctc atccttatgc ggggtgacga
    26161 aatggtgatc tgacggctct tcgacgaaca tgacatcgtc gtcaccggga accgcagtcg
    26221 ggatatagcc gtcggcgccg ccggcattga gcgcgctgtg gcggcgaccc cagaacaagt
    26281 agaacagcag cacgaggccg acacaggccc cgaacaccag ccatgtctgc cagcgcaggc
    26341 cgtacagcac cgccgcgcac gcgaggacgg agaggacggg tgtcacgggg taacccggca
    26401 ccttgaatgg gcgctcgagg ttgggctcgc ggactcgcag gatgatgacg cccaccgaca
    26461 ccgtgatgaa ggcgaccagc gtgccgatcg acacggcatc cagcagccag ttcagcggaa
    26521 tgaaaccggc aaggatcccc gtcgcgaccg cgacgatgat ggtgttgccg accggagtca
    26581 tgctgcgcgg gttgaccttt gcgaacatcg acggaagcag tccgtcgcgg cccatcgcga
    26641 acaggatgcg ggtctggccg tacatcacga ccagggtcac cgagaagatc gagatcaccg
    26701 ctcccgcggc cagaagcgtg ctggcccacg ttccgccggt gatgttctcc aggatcaccg
    26761 acagtcccgc ttcggactgt tcctccgacc cgaactcggc ggcgtcctgc gtgccgagtc
    26821 cggcgaaggc gaccaggatg tagaagctgg tgaccacgat cagtgcagca atgatcgcgc
    26881 ggggcatggt cttctgcgga tccttcacct cgtcaccggc tgtcgatacc gcgtcgaggc
    26941 cgatgaacga gaagaagatc atgctcgcgg cggtggtgat gcccgccatg cctttgtccc
    27001 agaagccgtg gaagtgatcg gcgttgaatg cagtgagcgc gatgatgatg aacatcccca
    27061 gcacgccgag cttgatcagc accatgatgg tgttgaccaa agccgactcg ctggcaccgc
    27121 ggatcagcag cagcatgcac atcacgatca gcaagacagc gggcaggttg acgatgcccg
    27181 gcgtttcatc ccagggggcc gccgtcagtg actgcgggag ttgccaacca aacaggttgt
    27241 ccaacaactt gttcaggtaa ccgctccacc cgaccgcgac tgcggagata gacacgccat
    27301 actcaagcag cagacaggct gccacaccca tcgcgacgaa ctcgcccatc gtcgtgtagg
    27361 cgtaggagta cgtcgagccc gacaccggca ccgacgacgc catctccgca tagcagatgg
    27421 cggacaggcc ggctgcgatc gcggcgagga cgaacgagac gaggaccgcc ggacccgctt
    27481 ccggcaccgc cgccgacagc acgaagaaga tgccggtgcc gacggtggcg ccgacgccga
    27541 acagcgtcag ctgaaacgtt ccgatacttc gtttcaggtg gtctgaggca ccgtgtgcga
    27601 tggg
//
LOCUS       DASRJA010000229         4732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2257182,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000229 DASRJA010000000
VERSION     DASRJA010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2257182"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2281)
                     /gene="glgP"
                     /locus_tag="VFC01_10365"
     CDS             complement(<1..2281)
                     /gene="glgP"
                     /locus_tag="VFC01_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HZN80075.1"
                     /translation="MKALRRFTVRAHLPDRLTALERLSINLRWSWDKPTQDLFAAIDP
                     ELWKQVGCDPVALLGQVTPKRLDELAVDESFLRRLDGLAADLDNYLTRPMWYQQQADE
                     GAQLPHGIAYFSMEFGVAEVLPNYSGGLGILAGDHLKSASDLGLPLIAVGLLYRSGYF
                     RQSLTADGWQHESYPSLDPQGLPLRLLTDPNGDPVLVELAMPDANQLRARVWIAHVGR
                     IPLLLLDSDIPENEHDLRSVTDRLYGGDQEHRIKQEILAGIGGVRAIRAYTEIEGLPA
                     PEVFHMNEGHAGFLGVERIRELIDAGLDFDTALTVVRSSTVFTTHTPVPAGIDRFPVE
                     MVKRYFGGSADGPSGAMSRLLPGVPLDRITAFGEEDDPSKFNMAHMGLRLAQRANGVS
                     LLHGRVSRFMFNELWPGFDAAEVPIGSITNGVHGPTWAAPQWLELGRELLGSDDLGSL
                     TEPETWQRLQQVDPGHIWWIRSQLRASLVEDVRARLRRSWHDRGASDAELGWIATAFD
                     PDVLTIGFARRVPTYKRLTLMLRDPDRLEKLLLDEKQPIQLIVAGKSHPADDGGKALI
                     QQVVKFADRPEVRHRIAFLPDYDMSMARQLYYGCDVWLNNPLRPLEACGTSGMKSALN
                     GGLNLSIRDGWWDEWYDGENGWEIPTADGLADEGRRDDIEAAALYDLLGKSVAARFYD
                     RNEHGIPTRWVEMVRHTLIALGPKVLASRMVRDYTEQYYAPAAQSLRKTVEPGADGEP
                     FGAARELAAYRQRTQEAWPK"
     gene            2473..4560
                     /locus_tag="VFC01_10370"
     CDS             2473..4560
                     /locus_tag="VFC01_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HZN80076.1"
                     /translation="MAGRIGIDDVAPVVSGGRYPAKAVVGEIVPVRATVWREGHDAVS
                     ATLVVRYHGRAYPKLADDPPGRVKPAEPVPIQEVVTPAPRVKPQQLPMSMGRTPDVFH
                     GFFSPTDVGLWTYRVDGWGDPFATWRKNVTAKLDAGQSESELSNDLLLGARLLERAAT
                     GAPRQHRYPLIDAAARLRAPGDPFSRAGAALSDDVAEVLALYPLRELLTRGEQYGVWV
                     DRPLARFSSWYEMFPRSSGGWDAKGNPVHGTFATATKELPRIAKMGFDVVYLPPIHPI
                     GKVHRKGRNNSVTAAPNDVGSPWAIGSDEGGHDAVHPKLGTIEDFDDFVAASRDHGLE
                     VSLDLALQCAPDHPWAKNHPEWFTVLPDGTIAYAENPPKKYQDIYPLNFDNDPAGIYE
                     EVLRVMRFWISHGVKVFRVDNPHTKPPNFWAWVIGEVKNEDPDVLFLSEAFTRPARLY
                     GLAKLGFTQSYTYFTWRTAKWELTEFGQQIAEHADEARPNLFVNTPDILHESLQHGGP
                     GMFAIRAVLASTMSSAWGVYSGYELFEHRPVREGSEEYLNSEKYELRPRDFDAALADG
                     ESLEPFLTRLNEIRRVHPALHELRTIKFHHPDNDAVLAYSKFDPVTGDQVLVVVTLNP
                     FGPEEATLWLDMNALGMEPYDRFWVRDEISGDEYQWGQSNYVRLDPAKAVAHVLNMPQ
                     VPADQRLNLLRRE"
BASE COUNT          837 a         1556 c         1543 g          796 t
ORIGIN      
        1 tcttgggcca ggcttcctgc gtccgctgcc ggtaagccgc cagttcgcgt gccgcgccga
       61 acggctcccc gtccgcgcca ggttcgacgg tcttgcgcag cgactgcgcc gcgggcgcgt
      121 aatactgctc ggtgtagtcg cgcaccatcc gcgaggccag caccttcggc ccgagcgcaa
      181 tgagcgtgtg ccgcaccatc tctacccatc gcgtcgggat gccgtgctcg ttgcggtcgt
      241 aaaacctcgc cgccaccgac ttgccgagca ggtcatacag cgcggcggct tcgatgtcgt
      301 cgcggcggcc ctcgtcagcc agaccgtcgg cggtcgggat ttcccaaccg ttttcgccgt
      361 cgtaccactc atcccaccag ccgtcgcgga tcgacaggtt caacccgcca ttgagcgcgc
      421 tcttcatgcc tgatgtgccg catgcctcca gcggccgcag cgggttgttc agccagacat
      481 cgcagccgta gtacagctgg cgggccatcg acatgtcgta gtcgggcagg aatgcgatgc
      541 gatgccgcac ttctggacgg tcggcgaact tcaccacttg ctggatcagc gccttgccgc
      601 cgtcgtctgc gggatgcgac ttgccggcca cgatcaactg aatcggctgc ttctcgtcga
      661 gcagcagctt ctccaaccgg tcggggtcgc gcagcatcaa cgtcagccgc ttgtacgtcg
      721 gcacgcggcg ggcgaaaccg atagtgagta cgtcggggtc gaatgccgtt gcgatccagc
      781 ctaattccgc atcggatgcg ccacggtcgt gccatgagcg tcgaaggcgg gcccgcacgt
      841 cctcgacgag ggaagcgcgc aattgtgagc ggatccacca tatgtgcccc ggatcgactt
      901 gttgtagccg ctgccacgtt tcgggctccg tgagagagcc gagatcgtcg ctgcccagca
      961 gttcgcggcc cagctccagc cattgcggcg ccgcccacgt cggcccgtgc acaccgttgg
     1021 tgattgagcc gatcggcacc tcggccgcat cgaaccccgg ccacagctcg ttgaacatga
     1081 agcggctgac ccgcccgtgc agcagcgaca ccccgttggc gcgctgcgcg agccgcaggc
     1141 ccatgtgggc catgttgaac ttggacggat catcctcctc gccgaacgcg gtgatccggt
     1201 ccagcggtac gcccggcagc agcctcgaca tcgcacccga cgggccgtcg gccgagccgc
     1261 cgaagtaccg cttgaccatc tcgaccggga atcggtcgat gccggcgggc accggggtat
     1321 gcgtggtgaa cacggtcgac gaccgcacga ccgtcagcgc cgtgtcgaag tccagcccgg
     1381 cgtcgatcag ctcacggatg cgttcgacgc ccagaaagcc ggcgtggccc tcgttcatgt
     1441 ggaagacctc gggcgcaggt agaccctcga tttcggtgta cgcgcggatc gcccgcacgc
     1501 cgccgatgcc ggccaagatc tcctgcttga tccggtgttc ctggtcaccg ccgtagagcc
     1561 ggtcggtgac actgcgcaaa tcgtgctcgt tttccggaat gtcggaatcc aacagcagca
     1621 acggaattcg gccgacgtgc gcgatccaca cacgcgcccg cagctggttg gcgtctggca
     1681 tcgccaactc gaccaacacc gggtcgccat tggggtctgt cagcagccgc agtggcaggc
     1741 cctgcggatc cagcgacgga tagctctcgt gctgccaccc gtcggcggtc agcgactggc
     1801 ggaagtaccc cgacctgtac agcaacccga ccgcgatcag gggcagcccg aggtccgacg
     1861 cggacttgag gtggtcaccc gccagaatcc ccagaccacc ggagtagttc ggcagcacct
     1921 cggcaacacc gaactccatc gaaaagtagg cgatgccatg cggcaactgc gcgccctcgt
     1981 cggcctgctg ctggtaccac atcggccggg tcaaatagtt gtccaggtca gcggccagcc
     2041 cgtcgaggcg gcgcaggaag gattcgtcga ccgcaagctc gtccagccgc ttcggtgtga
     2101 cctgtccgag caacgccacc gggtcgcatc cgacctgctt ccacagctct ggatcgatgg
     2161 ccgcgaacag atcctgcgtc ggcttgtccc acgaccagcg caggttgatc gaaagacgtt
     2221 cgagcgcggt caaacgatcg ggtagatgtg cgcggaccgt gaaccggcgg agggctttca
     2281 cgcgacccaa ccttactgat aaatcgggcc tttgcagggc ggtgtgggga tcgcttcgtt
     2341 cccgttgggt tgggctgacc actacggtgg gtataggcgc gacgaggctt taattcgaga
     2401 ccagacgaca agcacagcgg ccacgtcggc ggtgccacct ccgcgggccg cgccagtgta
     2461 aggagtgatg gggtggccgg tcgtatcgga atcgatgacg tcgcacccgt cgtatcaggc
     2521 ggacgctatc cagccaaggc cgtcgtgggt gagatcgttc ccgtccgggc cacggtgtgg
     2581 cgcgaaggac acgacgcggt atccgcgacg ctcgtggtgc gctaccacgg cagggcctat
     2641 ccgaaactgg ctgacgatcc gcccggacgc gtcaagccgg ccgagcctgt gccgatccag
     2701 gaggtggtca ccccggcgcc gcgggtgaaa ccgcagcaat tgccgatgtc gatgggccga
     2761 acgccggacg tgttccacgg ctttttcagt cccaccgatg tcgggttgtg gacgtaccgg
     2821 gtggatggct ggggcgaccc gttcgcgaca tggcgcaaga acgtcaccgc caagctcgac
     2881 gcgggccaga gcgagagcga gctgtccaat gatttgctcc tcggcgcccg gcttctggag
     2941 cgggccgcaa cgggcgcacc gcggcagcac cgctatccgt tgatcgacgc ggccgctcgc
     3001 ctgcgtgcac ccggtgaccc gttcagccgc gccggtgccg cgctgtcgga cgacgtggcc
     3061 gaggtgctcg cgctgtatcc gctgcgtgaa ttgctcacgc gcggagagca atacggtgtc
     3121 tgggtagacc ggccgctggc ccggttcagc tcgtggtacg agatgtttcc gcggtccagc
     3181 ggcggctggg acgcgaaggg caaccccgtg cacggcacat ttgcgaccgc caccaaggaa
     3241 ttgccccgca tcgccaagat gggcttcgac gtggtgtatc tgccgccgat ccatccgatc
     3301 ggcaaagtgc accgcaaagg ccgcaacaac agcgtgaccg ccgcgccgaa cgacgtcggc
     3361 tcaccctggg cgatcggcag cgacgaaggc ggccacgatg ctgtgcaccc gaagttgggc
     3421 acgatcgagg acttcgacga ttttgtggcc gcctcccgcg accacggctt ggaagtgtcg
     3481 ctggatctgg cgttgcaatg cgcgcccgac cacccatggg cgaagaacca cccggagtgg
     3541 ttcactgtgc tgcccgacgg gacgatcgcc tacgcggaga atccgccgaa gaagtaccag
     3601 gacatctacc cgttgaactt cgacaacgac cctgcgggga tctacgagga agtgctgcgg
     3661 gtaatgcggt tctggatctc ccacggcgtc aaggtgtttc gcgtcgacaa tccgcatacc
     3721 aagccgccga acttctgggc ctgggtgatc ggcgaggtga agaacgagga tcccgacgtg
     3781 ttgttcttgt cggaggcctt cactcggccg gcgcggctgt acgggttggc caagctcggc
     3841 ttcacgcagt cctacacgta tttcacctgg cgcaccgcga agtgggagct gaccgaattc
     3901 ggccagcaga tcgccgagca cgcggatgag gcacggccga acctgttcgt gaacacgccc
     3961 gacatcctgc acgagagcct gcagcacggc ggccctggca tgttcgcgat ccgggccgtg
     4021 ctggcgtcga cgatgagttc ggcgtggggc gtgtactcgg gatacgagct gttcgaacac
     4081 cggccggtgc gggaaggcag cgaggaatac ctgaactccg agaagtacga actgcggccc
     4141 cgcgacttcg acgccgcact ggccgacggt gaatcgcttg agccgttcct gacccggctc
     4201 aacgagattc gacgcgtcca cccggccctg cacgagttgc gcaccatcaa gtttcaccac
     4261 cccgacaacg acgcggtgct ggcctacagc aagttcgacc cggtaaccgg cgaccaagtg
     4321 ctggtggtgg tgacgctgaa cccgttcggg ccggaagagg cgacgctgtg gctcgacatg
     4381 aacgcgctgg ggatggagcc gtacgaccgg ttctgggtgc gtgacgagat cagcggggac
     4441 gaataccaat gggggcaatc gaattacgtt cgactcgacc cggccaaggc ggtggcccat
     4501 gtgctgaaca tgccccaggt acccgccgac caacgactca acctactgcg tagggagtga
     4561 cgagatgacg cagaccaatc aacgcacgag cccgcatctg cggccgcata cggcggatct
     4621 gaaccgcttg ctggccggcg agcaccatga tccgcattcg gtgctgggcg ctcacgagta
     4681 cggcgatcac accgtgattc gggcctatcg gccgcatgcg gttgaggtgg tc
//
LOCUS       DASRJA010000230         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_239391,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000230 DASRJA010000000
VERSION     DASRJA010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239391"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFC01_10375"
     CDS             <1..336
                     /locus_tag="VFC01_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80077.1"
                     /translation="GAAFLAVGLVIGAVTYVVVRKFRRRRSRPLHECRPVAVIDRKLG
                     LFSYGGGAVVPTDQVRFARRFQIGSSSPKLVAVTQGGTHVLKRGNPFDGGVGNVDELL
                     TAVAKNAVT"
     gene            382..1440
                     /locus_tag="VFC01_10380"
     CDS             382..1440
                     /locus_tag="VFC01_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HZN80078.1"
                     /translation="MTQLSRRSVLLGLGAVGAFLAVDFGAVAYANKWIGPQAELTRQT
                     FIDGFRRVYGLHPGFRRNHAKGVAVTGRFDSSGNGSELSRAAVFGPGHSSVTGRFSLS
                     GGDPFMADTAAAARGLGLAFAFPDGTQWRTAMLNLPVFPDNSPQGFYDRLLASKTVPA
                     TGKPDPAAMSAFLTAHPETAAAMAVIKQHAPTRGFADSTFSSLTTFYLVNDAGRRTPV
                     RWSFVPLQAALPAPPGSDNALFDALIRQIRGVPLQWRLMLTVGEPGDPVLDATLPWPS
                     DRRTIDAGTLTLTGVKTERRGNARDINFDPLVLPDGIEPSDDPLLSARSAVYAASYRA
                     RTGEPKFPSAVQVDEVAQ"
     gene            1437..2069
                     /locus_tag="VFC01_10385"
     CDS             1437..2069
                     /locus_tag="VFC01_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HZN80079.1"
                     /translation="MTARYPLRTRILHWLTAVLIFSTLLIGFVMVNSIGSYASLRAIH
                     MTLGVLILAIVVLRAANRLTHRAPKLPDTVGWVEHKLVVGSELTLYALMLAQPLIGWA
                     MVSASGRPVVIFGSLQLPRIAPFNADLFFILRQAHSVLAYALVVVIAAHVSAVLLHTL
                     TLRDGMLSRMTFTKPRERAQNARNSRRVGVQTRTSPTGVWAHAEKEARRG"
     gene            complement(2056..>3124)
                     /gene="hisS"
                     /locus_tag="VFC01_10390"
     CDS             complement(2056..>3124)
                     /gene="hisS"
                     /locus_tag="VFC01_10390"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZN80080.1"
                     /translation="YTFADRGDRSVTLRPEGTAGVVRAVIEHGLDRGPLPVKLCYAGP
                     FFRYERPQAGRFRQLQQVGVEAIGVDDPALDAEVIAIADAGFRSLGLDGFRLEITSLG
                     DDSCRPQYRELLQDFLFKLDLDEETRRRAEINPLRVLDDKRPKMREMTADAPVMLDHL
                     SDVAKQHFDTVLAHLDALSVPYVINPRMVRGLDYYTKTTFEFVHDGLGAQSGIGGGGR
                     YDGLMRELGGKDLSGIGFGLGVDRALLALKAEGKTVGGATRVDVFGVPLGEQAKLTLA
                     VLAARLRAAGVRVDLAYGDRGIKGAMRAADRSGASIALVAGDRDIEAGTIGVKNLATG
                     EQVDIPIDAVLAEVLSRLSRA"
BASE COUNT          534 a         1058 c          984 g          548 t
ORIGIN      
        1 ggtgcggcat tcctggccgt cggcctggtc atcggagcag tgacctatgt cgtggtgcgc
       61 aagtttcgcc gccgccgcag ccggccacta cacgaatgcc gcccggtggc ggtcatcgac
      121 cgcaagctgg ggttgttcag ctacggcggc ggagcggtgg ttccgacgga ccaggtgcgg
      181 tttgcacgca gattccaaat cggttcgagc tcacccaaac tcgtcgccgt cacccagggc
      241 ggtacgcatg tgctgaagcg cggtaaccca ttcgacggtg gggtcggcaa tgtcgacgaa
      301 ttgctgaccg cggtggcgaa gaatgctgtg acctagcgca caacgctgaa gcaatccgcg
      361 ccgcgccgcg ttttgcagtt cgtgacccaa ctcagtcgac gctcggtgct gctcggtctc
      421 ggtgcggtcg gcgcattcct ggccgttgat ttcggtgccg tcgcctatgc gaacaaatgg
      481 atcggcccgc aagccgagtt gacccgccag acctttatcg acggcttccg ccgcgtgtac
      541 ggtctgcacc cgggattccg caggaaccac gccaaaggcg tcgcggtgac cgggcgcttc
      601 gacagcagcg gcaacggcag cgaactatcg agggcggcgg tgttcggccc tggtcacagt
      661 tcggtgaccg ggcgattctc gctgagtggc ggcgatccct tcatggccga cacagccgcg
      721 gcggcgcgag gcctcggatt ggccttcgcg tttcccgacg gcactcagtg gcggacagcg
      781 atgctgaatc tgccggtgtt tcccgacaat tcgccgcaag gcttctatga ccggctactc
      841 gcatccaaaa ccgttcctgc gacggggaag cccgatccgg cagcgatgtc tgcgttcctg
      901 acggcgcacc ccgagacggc ggccgccatg gcggtaatca aacagcacgc accaactcgc
      961 ggcttcgccg acagcacatt cagcagcctc accacgttct acttggtcaa cgacgctggt
     1021 aggcgcacgc ccgtgcggtg gtcgttcgtc ccactgcagg cggcactgcc ggcgccaccg
     1081 ggcagcgata acgcattgtt cgacgcgctg attcgtcaga tacgcggcgt gccactgcag
     1141 tggcgattga tgctgacggt cggggaacca ggcgaccccg tactcgacgc cacgctgcca
     1201 tggccgtccg accgccggac catcgatgcg gggacgctca cgctgacagg cgttaaaacc
     1261 gagcgacgcg gcaatgcgcg cgacatcaac ttcgatccgc tcgtgctgcc cgacggcatc
     1321 gaaccgtcgg acgatccatt actgagcgct cgatcagcgg tgtacgcggc gtcgtatcgt
     1381 gccaggacgg gcgaacctaa gtttccgtcc gccgtgcagg tcgacgaggt cgcgcaatga
     1441 cggcgcgata tccgctgcga acgcgcatcc tgcactggct gacggccgtc ctcattttca
     1501 gcacgctatt gatcgggttc gtcatggtga actcgatcgg ctcttatgcg tcgctgcgcg
     1561 ccattcacat gacgctcggg gtgctgatac tcgccatcgt tgtcctcagg gccgccaacc
     1621 gattgaccca ccgcgcgccg aaactgcctg acaccgtcgg ctgggtagag cacaaactcg
     1681 tcgtgggttc cgagctgacg ctgtacgcac tgatgctggc gcagccactc atcgggtggg
     1741 cgatggtgtc ggccagcggt aggccggtgg tcatcttcgg gtccctgcag ctgccgcgca
     1801 ttgcgccgtt caatgccgac ttgtttttca tactccgcca agcccattcg gtgctggcct
     1861 acgcgttggt ggtggtcatc gccgcccacg tgagtgcggt attgctgcat acgctgacgc
     1921 tgcgtgacgg catgctgtct cggatgacgt tcaccaaacc gcgcgagcgt gcgcaaaatg
     1981 cacgcaattc gcggcgtgtc ggtgtgcaga cacgcacgtc ccctactggc gtgtgggccc
     2041 acgcggaaaa agaggctagg cgcggctgag gcgcgaaagc acttcggcga gaacagcgtc
     2101 gatgggaatg tcgacctgtt cgccggtcgc caggttcttc acgccgatcg tgcccgcctc
     2161 gatgtcacgg tcaccggcga ccaaggcaat cgacgcgcca gagcggtctg cggcgcgcat
     2221 ggcgcccttg atgccgcggt cgccgtaggc caggtcgacg cgcacaccag ccgcccgtag
     2281 gcgcgcggcc agtacggcca gcgtcagttt ggcctgctcc cccagcggaa ccccgaacac
     2341 gtcgacccgc gtggccccgc cgaccgtctt accctcggcc ttcaacgcca acaacgcccg
     2401 atcgacgcca aggccgaagc caatgccgga caagtctttt ccgcccagct cgcgcatcag
     2461 cccgtcgtag cgcccaccgc cgccgatgcc cgactgggcg cccagcccgt cgtgcacgaa
     2521 ctcgaacgtc gtctttgtgt agtaatccag cccgcggacc attcgcgggt tgatcacata
     2581 gggcacgctc aacgcgtcga gatgcgcgag caccgtgtcg aagtgctgct tggccacatc
     2641 ggacaggtga tccaacatca ccggcgcatc ggcggtcatc tcgcgcatct tcggccgctt
     2701 gtcgtcaagc acccgcaacg ggttgatctc ggcgcgccgg cgggtttctt cgtcgaggtc
     2761 gagcttgaac agaaagtcct gcaacagttc ccgatactgc ggccgacagc tgtcgtcgcc
     2821 cagcgaggtg atctccaacc ggaacccgtc gagccccagt gagcggaacc cggcgtctgc
     2881 gatggcgatc acctcggcgt ccagcgccgg atcgtcgaca ccgatcgcct cgaccccgac
     2941 ctgttgcagc tgtcggaaac gcccggcctg cggacgctcg taacggaaga acggacccgc
     3001 gtagcagagc ttcaccggca gcggcccgcg gtcaaggccg tgctcgatca ccgcgcgcac
     3061 cacacccgca gtgccctcgg gccgcagcgt caccgagcgg tcgccacggt cggcgaacgt
     3121 gtac
//
LOCUS       DASRJA010000231        25667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1736953,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000231 DASRJA010000000
VERSION     DASRJA010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 25667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25667
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736953"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..446)
                     /locus_tag="VFC01_10395"
     CDS             complement(111..446)
                     /locus_tag="VFC01_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80081.1"
                     /translation="MKITSAVAAALAFSLAGAASALADEIGGDASGGGDFGFGVSGPT
                     LPPPPPPPALPAPTASASGGTGVGVDTPLGSAEGGFATDLGVGLPTLPPPPPPSASGS
                     AQTNLGFGF"
     gene            complement(924..2318)
                     /locus_tag="VFC01_10400"
     CDS             complement(924..2318)
                     /locus_tag="VFC01_10400"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN80082.1"
                     /translation="MAPTPTHAPEAASTPDFATVSTLINDAIAAHRLPGAVVVIGHGG
                     RVVFHQAYGSRKLAGEPGLDGSPAPAEQMTEDTIFDMASLTKPIATATAVMQLYEQGK
                     VAFDDPVQQYLPDFNTANDPQRAKVTVRMLLTHTSGETGDVDLKDPWGLDGADKTEGI
                     HRALTTPLESGPGERFRYSDINFILLGALLESVTGEAEDVYVQQHVFAPLGMEETRYL
                     PPAKACGPYTLRGAAIAWAPDERVAGACSAGTWNIDLLSRIAPTARDEEGRADPGKNP
                     DLDYLLRGTVHDTTARRMGGVAGHAGVFSTAHDVSIYAQALLDRLTGRPSTFPLTQAT
                     LELMTTPQQPGHTDEQLEAANDATRDAVAKRPNTTDPLLAPHYPAIKGQNLRGFGWDI
                     DSTNSPPRGIVFPIGSFGHTGFTGTTLWIDPGSDTYVVLLSNSIHVRGSPPIQDLRGD
                     VATATAQALRLYGN"
     gene            complement(2471..2935)
                     /locus_tag="VFC01_10405"
     CDS             complement(2471..2935)
                     /locus_tag="VFC01_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZN80083.1"
                     /translation="MNRRETTEARAARQKEFEEDARRLREFPVFAKFSEDDLKRIARA
                     THRTATSGPWPLIREQTVSDACYILLSGEAGVYVGRERIAVVGPGEVIGESALHRGKL
                     RNATVTTTGRAEVLHIARDDLSRLLDEVPALREVMDATVARHVPGAATTDPS"
     gene            complement(3107..3625)
                     /locus_tag="VFC01_10410"
     CDS             complement(3107..3625)
                     /locus_tag="VFC01_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5994 family protein"
                     /protein_id="HZN80084.1"
                     /translation="MTLKQDRTDVGQRNTPPEHTPRLRLKPKAPQSGYVDGAWWPHSD
                     DLAVELPDLLAVLSVRLGRIDRVMYNLNEWGRQSAKLATGGRLVRLNGYGRQPTNTIE
                     VLGVTGSRIVLLVVPPDTDPDAAHEAMMTAAAPKDTSMVGNLLAVGAHEQQGPNTCYH
                     PTYHRRVRRFAK"
     gene            complement(3776..5002)
                     /locus_tag="VFC01_10415"
     CDS             complement(3776..5002)
                     /locus_tag="VFC01_10415"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HZN80085.1"
                     /translation="MAVADIAAYAHLSQADIEALGDELDAIRRDIEDSLGESDAAYIR
                     RTIGFQKMLDVTARLLIGCSRSRVSWALGTAALAYAKSVENMEIGHNVSHGQWDWMND
                     PEIHSGTWEWDMVAVSSQWRYSHNYRHHVFSNVVGVDDDLGFGVLRVTRDEKWRPEHL
                     VQPLRNLLLAAIFEWGIALHGVHSERDRAADVGEVAGARAALIRKIGRQAGKDYVFFP
                     ALSRSRWRRTLAANATANLLRNLWAYVVIFCGHFPDGADKFTADVLKDETRAEWYLRQ
                     MLGAANFKAGPLLAFSSGNLCYQIEHHLFPDLPSNRYAQIAVRVRALCDKYDLPYTSG
                     SLVHQYLLTLRTIHKLALPDRLLRATCDDAPETASERKFGFGAAPATRSVGPIVGLRT
                     ALRTRARMRRRQRGCS"
     gene            complement(5299..5502)
                     /locus_tag="VFC01_10420"
     CDS             complement(5299..5502)
                     /locus_tag="VFC01_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZN80086.1"
                     /translation="MTEGTVKWFNSDKGFGFITPDDGTKDVFVHHSEIQTSGYRSLEE
                     SQRVKFDVNQGPKGPQAVGVTGI"
     gene            complement(5607..6155)
                     /locus_tag="VFC01_10425"
     CDS             complement(5607..6155)
                     /locus_tag="VFC01_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5994 family protein"
                     /protein_id="HZN80087.1"
                     /translation="MTLVQDRTDVGQRNPPPAHTLRLRLKPKASQSGYVDGAWWPRSD
                     DLATELPDLLAVLSVRLEPVDRVIFNRNEWAIPSADFATGGRRVRLDGYRLQPANTVE
                     VLGLNRNKIVLLVVPPNAHPDKAHATLMAAAAPSNDSTVDSLLMVSAREREIRTQATD
                     AQERWRSAGNEARAPRASSSSS"
     gene            complement(6367..7113)
                     /locus_tag="VFC01_10430"
     CDS             complement(6367..7113)
                     /locus_tag="VFC01_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HZN80088.1"
                     /translation="MTKQHAARGAVAAGSDAATVATARDDDSQVSRQGILQSALRIVD
                     RDGVDGLSLRRLSDEVRRDPTVIYRHVPNKAALLDGVTEIVLGQLRVNTVDPDWMGQL
                     RAVAHDFRRLALAHPNVVSLLVTRPLSTPLGQRPLGMLRPLEDVLTLLTSAGFSGADA
                     LHIYRVLFGYLHGHILTEIQEVIERPEETDDVLRLGLHRLPVTEFPQVRALASALANY
                     DGAAELDRGLDILLFGLTATLTPPDGPPRA"
     gene            7329..7592
                     /locus_tag="VFC01_10435"
     CDS             7329..7592
                     /locus_tag="VFC01_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80089.1"
                     /translation="MDGYQPSGAGRTLFLFGIAVGAVTSLPLRLLLTNLWPSASRAAD
                     ARRAVARRDHETAFINQDRDTKLEHQQRADTTSGNALTQALRI"
     gene            7602..7988
                     /locus_tag="VFC01_10440"
     CDS             7602..7988
                     /locus_tag="VFC01_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80090.1"
                     /translation="MATNTMVLIIVTTLAVLVLAGVFAGVVYKTRTPRWGEMVRDQVE
                     EDALRLRRQQTLADEFDARAHAAQVEIDIKTVRASSLQHQATAYRSEAVTSGDQLNEM
                     RGGAMRGSADKLTAAAQPPTVPRLTG"
     gene            complement(7999..8328)
                     /locus_tag="VFC01_10445"
     CDS             complement(7999..8328)
                     /locus_tag="VFC01_10445"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HZN80091.1"
                     /translation="MPWWKTFQGPGWSRLHDVTRWGATVGAHNAQILADALSLTAQLQ
                     TALSTRPVIDQAIGLIRGRTGRSVEDAFAHLRAMSQTEHRKLADVAQQIVDEAVRRAR
                     ARHRPTE"
     gene            8334..9512
                     /locus_tag="VFC01_10450"
     CDS             8334..9512
                     /locus_tag="VFC01_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(5)-(carboxyethyl)ornithine synthase"
                     /protein_id="HZN80092.1"
                     /translation="MTSTAETQQLSLGVMARSRKPNERRLPIHPAHFSRIDRRVRPRI
                     FLEHGYGSDFGATDVALAPLVGGIGSREQLIADCDIILLPKVQSEDLSELNVGQIVWG
                     WPHCVQDTALTQIAIDQRLTLIAFEAMNHWQADGGFGLHVFHKNNELAGYCSVLHAMQ
                     LAGITGSYGRRLRAAVIGFGATARGAVTALNAHGVDDVRVLTNREVAAVGSPIHSTQI
                     VQMGQDEEAPERTWADTPEDGVIPVARFLADNDIVVNCVLQDPAAPLIFVTDNDLQSF
                     TPGSLIVDVSCDVGMGFSWAHPTSFTQPIIELANGVHYYAVDHSPSYLWNSATWEISE
                     ALLPHIETVMAGPVMWNSTPTISRAIEIRDGVVLNPSILSFQHRDDDYPHLVRAEQSQ
                     "
     gene            complement(9730..10125)
                     /locus_tag="VFC01_10455"
     CDS             complement(9730..10125)
                     /locus_tag="VFC01_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80093.1"
                     /translation="MSKSGDKQRTIFRENVIHVGAGFAAKERPHVLAALSTLASHLGR
                     WNPRDVDVDVTLQDRGGKEQRVTLRTTLPGLPQLIAVAESAHITRALTEAKQELIRQL
                     EHQKSAREPMNSRRLRRATIRHAAADADG"
     gene            complement(10150..10308)
                     /locus_tag="VFC01_10460"
     CDS             complement(10150..10308)
                     /locus_tag="VFC01_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6307 family protein"
                     /protein_id="HZN80094.1"
                     /translation="MTSPTTFRTPYDLRLELVKDTITTHSKLGDKAAGELAVHVLHAL
                     NSIPEKIR"
     gene            complement(10411..11214)
                     /locus_tag="VFC01_10465"
     CDS             complement(10411..11214)
                     /locus_tag="VFC01_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZN80095.1"
                     /translation="MRREVGAELEVEITAPTTLEFQIAVAPHPNTSVSEALSFVLDGV
                     SVEPLEISGTHGNRIHKLDVPVGNLKVEYSAAIVGRTGPAPVTEYDLSMYLRPSRYAE
                     ADKFFGFAATEFGNYLDSTTLLAKVSSWVGTRLNYVPGSSDPIDGAVDTLLAGAGVCR
                     DFAHLVVALLRAVNVPARVVSVYAPGLSPMDFHAVAEAFVDGQWRVVDGTLLAPRQTL
                     VRIATGRDAADIAFLDNHKGAITLSKMEVTAVVDGDLPRDSIDQLVSIG"
     gene            complement(11228..12712)
                     /locus_tag="VFC01_10470"
     CDS             complement(11228..12712)
                     /locus_tag="VFC01_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HZN80096.1"
                     /translation="MTSLQVGLVTRSPQRLAADRSRVITRLFVPGQEGFEHQDSRAGA
                     VLARVLALDEDEVRASLDDVVARFDGRHRDLVGTFRRHAREVADRVDPASDLSDARML
                     LLGAVFTNEYAIEGAALCNPSMVAHPDQAGTPEGSLRFVMSVRGIGEGHRSSIGFRTG
                     VVDGADGVTIDDPAPLASVGPVVPTLLDGAVMRSELRRLHGAGEAADYVLDALGDQFT
                     RADLDEQVDKLLTHRRTRGRAAETISLIRTIADRTYGVEFDDGIPLSERVLWPAMSAE
                     GAGMEDARFVRFVDDDGSVTFYASYTAYSGSHISQQLLDTEDFRMFTSTPMVGPAAAN
                     KGLALFPRRIRGRFAAMCRSDRESNSIAYSDHPFEWTESVPCQQPARAWEALQLGNCG
                     PPIETEAGWLVLTHGVGPMRTYRIGAMLLDLDDPARILGQLHQPLLSPAVDEQDGYVP
                     NVVYSCGALVHGDTLVVPYGIGDAAIGVATVPLGELLAALVDYR"
     gene            complement(12709..13731)
                     /locus_tag="VFC01_10475"
     CDS             complement(12709..13731)
                     /locus_tag="VFC01_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN80097.1"
                     /translation="MTATVPAPVFDHLLRLTDRRGTLEHARLAEPLPEHGYCTDDVAR
                     VLVVATRDHGPDRTLNGLAGVSLRFLNDAQALDGGCRNRMDSTGNWVDEPTMEDAWGR
                     CIWGLGTAAAHSNVVWARQSAIVQFERAAQERSPWPRAMAFAALGAAELLAFDPEHRP
                     ARALLTDYAAAVPAPNTDSSWPWPEPRLTYANAVLPEAMIAAGDALADATLCQRGLDL
                     LAWLVEFETAHGHLSPTPVAGRGPDDARPGFDQQPIEVSSLADACARAAAVDINPIWR
                     DGVEAAAAWFMGDNDAGEPMWDPASGGGYDGLHADGVNNNQGAESTLAVISTLQHARR
                     LSTVRQ"
     gene            complement(13728..14864)
                     /locus_tag="VFC01_10480"
     CDS             complement(13728..14864)
                     /locus_tag="VFC01_10480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN80098.1"
                     /translation="MSHGFSGSPRFSILSTYPPTACGLATFSAALARGLTANGADVSV
                     VRVSDGPPSDSARVIGEMVNGSPTSVAACAELLNQSDVAVIQHEYGVYGGVDGVEVLD
                     VMDGLRVPSIVVAHTILKDPTRHQRSVLEALAARADQVIVMSEAARQRLSRGFDIDRR
                     KVATIPHGATIPTTASTVRRGRPTVLTWGLLGPGKGIERVIEAMASLSDVAGRPRYLV
                     AGQTHPKVLAADGEAYRDARMEQARRRGVADSVSFDTGYRSVAMLTDLIQSAAVVVLP
                     YDSTDQVTSGVLVDAIASGRPVVATAFPHAVELLGTGAGIVVGHDDPDAMASALRRVL
                     TQPRLAGAMAAEARRLAPEMAWPVVGSAYLRLAHQVLAQRRAWA"
     gene            complement(15367..15561)
                     /locus_tag="VFC01_10485"
     CDS             complement(15367..15561)
                     /locus_tag="VFC01_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80099.1"
                     /translation="MTVTVTLPDGGTDNYVRFGDAYVKNHDGTLDVVRTGAKHPFSYA
                     SGEWTDVEGDQKRFKKGFWR"
     gene            complement(15558..16082)
                     /locus_tag="VFC01_10490"
     CDS             complement(15558..16082)
                     /locus_tag="VFC01_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5994 family protein"
                     /protein_id="HZN80100.1"
                     /translation="MTRHEGGSVPGGRQTGPESTPRLRLKPKARKSGHVDGAWWPRSD
                     DLPNELPDLLAVLSVRLGAIDRVLYNLGEWAQMPKRLMTGGRAVRLDGYTRQPANTLQ
                     VLGVGREKLLLLVVPPHTDPDIAHEAMMTAAAPDDATTVGDLLGIDAQEQEEAPPNGY
                     HRPTFRRRVRRFRP"
     gene            complement(16513..16716)
                     /locus_tag="VFC01_10495"
     CDS             complement(16513..16716)
                     /locus_tag="VFC01_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZN80101.1"
                     /translation="MAQGTVKWFNGDKGFGFIAPDGDGADVFVHYSEIQGNGFKSLEE
                     NQRVEFTIEQGAKGPQAVGVTAV"
     gene            17043..17534
                     /locus_tag="VFC01_10500"
     CDS             17043..17534
                     /locus_tag="VFC01_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5994 family protein"
                     /protein_id="HZN80102.1"
                     /translation="MTREIHARRLSSPVRFTLASNLGSGLDGAWWPHTVSIARELSGL
                     ADAVRNPLGQVIDIGVNWSPLQGVPDLDLLNRRGVAATPGRDSRHLRVMTITGSRAKA
                     HLLVVPCGTSIALAVMLLRQAADLPVLYEHQHTSAFETAGVIVRNARAQNSPSAAPAE
                     TSG"
     gene            complement(17526..18857)
                     /locus_tag="VFC01_10505"
     CDS             complement(17526..18857)
                     /locus_tag="VFC01_10505"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN80103.1"
                     /translation="MTTTQSFADLGVGEPLVKALAARGILTPFPIQEKTLPDGLAGRD
                     VLGRGKTGSGKTLAFSIPLITRLAAMKRRSRPTGLVLAPTRELASQIAATLEPIAAAY
                     HLKVTTIFGGVPQNRQVAALRSGVDIVVACPGRLEDLMKQRVISLDTIDVTVVDEADH
                     MADLGFLPGVTRILAATPQGGQRLLFSATLDNGVDKLVKRFLRNPVTHSVDEAHSPVA
                     EMTHHVFHVAGVQAKKELVHTLASGSGRRILFMRTKHQARKLAKTLTESGVPSVDLHG
                     NLSQPARDRNLAAFSTGVARVLVATDIAARGVHVDDVELVVHVDPPMEHKAYLHRSGR
                     TARAGNAGDVVTVVLPEQRKDVQVLMRRAGINVRPQQVEADSTPVTELVGDVAPFQAP
                     AVLADQTPKPANTKTGGSRGRRSSSARRPRGGASAASSTHTGHSRRRSASA"
     gene            complement(19217..20194)
                     /locus_tag="VFC01_10510"
     CDS             complement(19217..20194)
                     /locus_tag="VFC01_10510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HZN80104.1"
                     /translation="MDNHHTDHGLTDPRRAVHQMHALVELLDDGVVVLRKDGYIKYVN
                     AAAMRIVGLGPEHKAADFARWTAALPIYDADGEPVPPQLRPVAQLFRTGVTFSRRVYG
                     TELPNGERKWMVASGRLLNPDAPGDLDMLFSFSDITAERAEVDRLVHKVNHDPLTGLP
                     NRGFVLRRIAEALAATDGRLRAVLFIDLDDLKTTNDTLGHDAGDDLLRAAGARLRQAV
                     GPADVVGRHGGDEFVVLIFGAETQGEIDSLVDRLRLRLAEPVVLADTTTPIRASVGLV
                     EVHRDDERTAEDILRDADRTMYAAKRGGRGDWCLERVRRGGRGPWPPRC"
     gene            20637..21935
                     /locus_tag="VFC01_10515"
     CDS             20637..21935
                     /locus_tag="VFC01_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN80105.1"
                     /translation="MPPLAQGPHLPRHGAAHASVVRELGFRPDQGIDAASAGVGLTPW
                     FQPVVSLPGEQVVGFEALARWPTFVTMTAHNVFSFANRSRHADVLDQQCIDAAVHAAL
                     DSELPRDSLLLINTEPAVAHVPLAKNPALTEAGERFQLAFELTERHMLAHPQTLLEKV
                     AAIRADGIAIAVDDVGAHPDSLAVLDIIDPDIIKLDMTMIQNCTMRNKADALTGVLAH
                     HERTGALIIAEGVETDEDLEQALAMGAGFAQGYRFGRARPLCRQPLPAGRPPAVRAQR
                     HPRSSAHAAAEDGRRPLRVARQHVVGAFTRHIEEQARHSVDQPIVLAALHRAHDFSAA
                     SRQLYQELAETSPLVVVLGRDMPADLGGGVRSVELQPEDPLCREWVVVALGADTCKAL
                     VARELADTAKHNGDRRFEFLVTSDRALVAKAARNLLARVA"
     gene            complement(21874..23142)
                     /locus_tag="VFC01_10520"
     CDS             complement(21874..23142)
                     /locus_tag="VFC01_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN80106.1"
                     /translation="MTNRAPLEEFDPGKLQGGLLSAVENVHEKLRVAREKHRVMVGNP
                     IVETPAQNLDMAGVTVLGYDECQTVLTHPENFSSSIYEHIMGPVMGKTLLQQEGDEHR
                     ASRALVSPLFRAKLLERWRTELVEVVVHELIDRFAPRGKADLVREFTFAFPVQVIARL
                     MGLPREDYVRFQQLSIELLSVVYDWDVGMGASATLKEYFGEILAERRKDPQDDLISTL
                     AESEIDGQRLSDDEIFAFLLLILPAGVETTYRASGNLLVAMLTEPSLLDTVRADRSML
                     RGAIEEALRWEPPISTVVRVARQDCELGGITIPKGTNVSVSVAAANRDPAHYPDPDRF
                     DPTRKNFAHLTFGGGPHVCLGMPLARMETTVAINALLDRLPDLHLDPSAPAPVINGVA
                     FRSPAALPVEFTPREPTGFARPSPPMHGRW"
     gene            complement(23139..24857)
                     /locus_tag="VFC01_10525"
     CDS             complement(23139..24857)
                     /locus_tag="VFC01_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN80107.1"
                     /translation="MLDLLIRGASIVDGTGAPARHGDVGVRDGRIVAVGEVDEPASKT
                     VDAEGQTLAPGFVDLHTHYDAQLFWDPTASPSVQHGVTTVFGGNCGFTLAPVADQQDY
                     LTRMLARVEGMPLEALRAGLDWQWSSFRDWLSRLDGRIAINAGFLVGHTAIRLAAMGD
                     DAVGGKASPEQIEKMATILSAALADGALGLSTSQSNTHNDGSGQPVPSRSASREELVA
                     LAAVLRNHPGTQLEAIIPGCLSGFTDDDIALLTDMSRAADRPLNWNVLGVSAGNPEGH
                     EKQLRASEVAAERGGRIVALTLPHSTKIRLSFLSGFVLDGLPGWRETMHLPVAERLRA
                     LADPEVRRRLNEGAHSEEAGILRGLANWERLIIVETFAPENDDATGRTVGEVAKARGK
                     EPFDTLLDIVIADELRTGLSPRPFGDDEADWKARAQVWRNRDAVIGGSDAGAHLDMMC
                     GAIYTTSLLGHGVREHQVVTLEEAVRLITDVPARLYGLKERGRIAEGWHADLVMFDPT
                     TVDHGPERTRYDLPAGAPRLVADAKGITGVWVGGVEVCRDGVATGAMPGTVLRSGRDT
                     ETVTAS"
     gene            complement(24842..>25667)
                     /locus_tag="VFC01_10530"
     CDS             complement(24842..>25667)
                     /locus_tag="VFC01_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN80108.1"
                     /translation="FWKSDDPELGLAVLRAWNDWHLEVWAGPYPDRIIPLQLPWLADP
                     QLAADELRRNADRGFKAVSFPELPAQCGLPSLHTGVWDPFLAACEETETVICLHTGSA
                     QWAPIPAPDTPFETITTLFPVNALVACADWLWSGIPVRFPKLNITLDEGGLGWAAMLA
                     DRADFVLAHSASGAEGGSWKGDLLPSEVLRRNFWFCSIDDPSAFGALDAIGAERILIE
                     SDYPHADSTWPDTQHVVARNVAKLSAEDAARITHRNAAELFRHPLPDDKWLTCSTS"
BASE COUNT         4545 a         8531 c         7964 g         4627 t
ORIGIN      
        1 cccatctgag gaacagtaag tggcggtaat caatgctgcc ggtgagtgcg ctggtgccaa
       61 aacatgggcc ccgtcctttt gggcggggcc catgtcgagc gattcacgtg tcagaagccg
      121 aagcccaggt ttgtctgggc cgaccccgag gccgacggcg ggggaggtgg aggcagcgtc
      181 gggagaccaa cgcccaagtc ggtcgcaaat ccaccctcag ccgaacccaa aggcgtatcc
      241 accccaacgc cggtccctcc ggacgcggaa gccgtgggcg ccggaagtgc cggaggcgga
      301 ggcggtgggg gcagcgtcgg gccactgaca ccgaacccga agtccccgcc gccggaagcg
      361 tcccccccta tctcgtctgc cagcgcgcta gcggcaccgg ccagcgagaa ggccagcgca
      421 gcggcgacgg cgctcgtgat cttcaaggtc gtacgtacag tcgattcaga caacaaattg
      481 ttccctctat cggttgttgg ttttggcacg accccccagt gggacgctaa caagatcgat
      541 cgccgggtgc aggtcgccaa cgtgtccggt ctgacatcag gaaacggttg ggccccaacg
      601 cgcgccggga aaaccccaag gtcgacggcg tccacgcctg cgtgcgggca aatgtcaaag
      661 tcgtggccga cggtgcactg ctgtgaaggc ccaggcggca tccttcatgc cgtgttgcag
      721 atcgacacag agagcgagcc gtcgcatggc gcaatgatgc cgacatgcaa cggccaacgg
      781 tgatcgaaca caacgttgtt gccgcaccag ctggcgcacg taccgccctt gggtaaacgt
      841 ggaaccgttt gcggcgccat acttttcaaa cggaccgcat cctggggcgg tgccgaccta
      901 cgccccccga cctcgtgacc ggactaattg ccgtagaggc gcagggcctg ggctgtcgcc
      961 gtcgccacgt caccccgcag atcctggatc ggtggactgc cgcgcacatg gattgaattc
     1021 gagagcagaa ctacatacgt atccgagccc gggtctatcc acagcgtggt tccggtgaag
     1081 ccagtgtggc caaagctacc gatgggaaag acgatgcccc gcggcgggga attggtcgaa
     1141 tcgatatccc agccaaagcc gcgcaggttt tgtcccttga tcgccggata gtgcggagcg
     1201 agcagcggat ccgttgtgtt cggtctcttt gcgacggcgt ctcgggtggc gtcgttcgcc
     1261 gcttcgagct gttcatctgt atgcccgggc tgctgcggag tcgtcatcaa ctcgagagtc
     1321 gcctgcgtca gcggaaacgt gctcgggcgg cccgtgagcc gatcaagcag ggcctgcgca
     1381 tagatgctga catcgtgcgc cgtcgagaac acaccggcat gcccggccac tcctcccatc
     1441 cgccgcgccg tcgtgtcatg caccgtgccg cgtagcaagt aatcaagatc gggattcttg
     1501 cccggatcgg ctctgccctc ctcgtcacgt gccgtcggtg cgatgcgcga caacagatcg
     1561 atgttccacg taccggccga acaagcgccc gccacacgct cgtccggcgc ccacgcgatc
     1621 gcagctcctc gcagcgtgta cggaccgcat gctttggctg gcggaagata tcgagtttcc
     1681 tccatgccga gcggcgcgaa tacgtgttgc tgaacgtaaa cgtcttctgc ctcgcctgtg
     1741 acgctctcca gcagcgcgcc aagcagaatg aagttgatgt cggagtagcg gaaacgctca
     1801 ccgggaccag actcgagggg cgtggtgagc gcacggtgaa tgccctcagt tttgtcggct
     1861 ccgtccagtc cccacggatc tttgaggtcg acgtctccgg tttcgcccga cgtgtgcgtg
     1921 agcaacatgc gaacggtcac cttcgcacgc tgtggatcgt tcgccgtgtt gaagtccggc
     1981 agatattgct gcacgggatc gtcgaacgca accttgccct gttcgtacag ctgcatgacg
     2041 gcagtcgcgg ttgcaatggg cttcgtcaac gacgccatgt cgaagatcgt gtcctcggtc
     2101 atctgctctg caggtgcagg cgatccgtct agtcccggtt cgcctgcgag cttgcgcgag
     2161 ccatacgcct ggtggaagac gaccctgccg ccgtggccga tgacaaccac agcacccggc
     2221 agccgatgcg ctgcaatcgc atcgttgatc agcgtggaga ccgttgcgaa gtcaggggtg
     2281 gaagccgcct ccggcgcatg tgtaggtgtg ggagccacat acggcacatg cgttggtgtc
     2341 gacgccgcag ttgacgttgc ctgcgggctg tcgctcactt ccgtgttcgc ggcacagccg
     2401 aacgaggagg ccgcagcgag tgcaaggacg acagctccga cgccgatggc ctttcgctgc
     2461 ccgctcccgt tcaacttggg tcggtggttg ccgctcctgg tacgtgtcga gccaccgtcg
     2521 cgtccatcac ttcacgcagt gcaggtacct catcaagcaa gcgtgagaga tcatcgcgcg
     2581 cgatatgcag tacttcggcc cgcccggtcg tcgtcacagt cgcattgcgc agttttccgc
     2641 ggtgaagcgc agattcgccg atcacctcac ccggccccac gacggcaatg cgctcccggc
     2701 cgacatacac acctgcctcg ccgctcagca ggatgtagca ggcgtccgac accgtctgtt
     2761 cacgaatcag cggccacggc cccgacgtcg cggtgcgatg tgttgctcgg gcgatacgct
     2821 tcaggtcgtc ctcggagaac ttcgcgaata cgggaaactc ccgtagtcgg cgagcgtcct
     2881 cttcaaattc cttctgacgg gctgccctcg cctcggttgt ctcacgcctg ttcatggtgg
     2941 ctcccccgat tcatgatgac ccggacatcc aggagcctag cgagagaccg ttgtctgcag
     3001 gccggaacgc gcactccgct cccccggcgc gaccatcagc cccagaaacg cttcttgaac
     3061 cgcatgtagt tgtgggtgcc accgtcgcgg agtgtcacgg taacggtcat ttcgcaaacc
     3121 gccggacgcg ccgatgatat gtcgggtggt aacaggtgtt ggggccttgt tgttcgtgcg
     3181 cgccaactgc gagcaggttg ccgaccatcg aagtgtcctt cggcgccgcc gcggtcatca
     3241 tcgcctcgtg cgcagcgtcc ggatcggtgt ccggaggtac cactagcaag acaatcctgc
     3301 tgccggtgac cccgagaact tcgatggtgt ttgttggctg acgtccgtac ccgttgagcc
     3361 gtaccagtcg gccgccagtc gcgagtttcg cggattgtcg cccccattca ttgaggttgt
     3421 acatcacgcg gtcaatgcgc ccgagccgaa ccgacagtac agccagcaga tctggcagtt
     3481 ccacggcgag gtcgtcactg tgcggccacc atgccccatc gacatatcca ctctggggcg
     3541 ccttcggctt caagcgcaat cgcggcgtgt gttccggcgg tgtattgcgt tgcccgacgt
     3601 cggtgcgatc ttgtttcagc gtcattgtga cgctcctgtc ttgaccgcga accggccaga
     3661 tcctacgaat cgacgacgac acaaccttga acggtcatat gacgaaatac cgccgatgta
     3721 ccttcagcct actcgggttg tccaccacag cggccagccg agttcgagat cgaaatcaag
     3781 aacatccgcg ttggcgtcgg cgcatgcgtg cacgagtacg caacgcggtt cgcagcccta
     3841 ctatcgggcc aaccgaacgg gtagccggcg cagccccgaa cccgaacttc cgttccgatg
     3901 cggtctccgg cgcgtcgtcg cacgtagccc tgagcaacct gtcaggaagc gccagtttat
     3961 ggatcgttcg cagcgtcaaa agatattggt gcacaagcga accgctggtg taaggcaggt
     4021 catacttgtc gcacagcgcc cgcacacgca ccgcgatctg agcgtagcga ttactcggca
     4081 ggtcgggaaa cagatggtgc tcgatctggt aacacaagtt gccgctcgaa aacgccagca
     4141 gcgggccggc cttgaagttc gccgcaccca acatctgccg cagataccac tcggcccggg
     4201 tctcatcttt cagtacatcg gcagtgaact tgtctgcgcc atcagggaaa tgcccgcaga
     4261 agatcaccac atacgcccac aggttgcgca gcaggttggc cgtcgcattt gcggccagcg
     4321 ttctgcgcca acgagaccgg ctcagcgccg ggaagaacac gtaatccttg ccggcctggc
     4381 ggccaatttt tcggatcagc gctgcccttg cgccggctac ctccccgaca tctgccgccc
     4441 gatcgcgctc cgaatggacc ccatgaaggg cgattcccca ctcaaagatc gccgccaaca
     4501 gaagatttcg cagcggctgc accaggtgct ccgggcgcca cttctcgtcg cgggttaccc
     4561 gcagcactcc gaagccgaga tcgtcgtcca cgcctaccac gttgctgaac acgtggtgtc
     4621 ggtagttgtg cgaatacctc cactgcgacg agaccgcgac catgtcccac tcccacgtgc
     4681 ccgagtggat ctccgggtcg ttcatccaat cccattggcc gtgagacacg ttgtggccga
     4741 tctccatgtt ctcgacgctc ttcgcatagg ccagagccgc cgtccccagg gcccaggaga
     4801 cccgtgaccg gctgcagccg atgagcaggc gcgccgtgac gtcaagcatc ttctggaacc
     4861 cgatggtgcg ccggatatag gccgcgtcac tttcaccgag cgagtcctcg atgtcgcggc
     4921 ggatcgcgtc cagttcgtcc ccgagagcct caatgtccgc ctggctcaga tgcgcgtacg
     4981 cagcaatgtc ggcaactgcc actaattgtc ccgttggtcc ggtcgtgcag gcgagtgtct
     5041 gagctctggt gtgaacccga aatgagtgcg gacgaccggt cgggttgatg gagacgacgg
     5101 ccgcgcgtcg gcgtaccgcc agtctagcgt cgcgccgcaa cgttgtgcgt cgacatcgtt
     5161 accaggccat gaacggttcc ccgtcgcagt tctacctgcg atggacgggt gccgtctgga
     5221 cggcagacga aaccccctga gctgacacgg cagagtgaac gcaggccagt cagctcagag
     5281 ggtttcccag tcggcgagct agatgccggt tactccgact gcctgaggcc ctttcggtcc
     5341 ctggttgacg tcgaacttga ctcgttgact ctcttcaagc gagcgatagc cgctcgtttg
     5401 aatctctgaa tggtgaacga acacatcctt tgtgccgtca tcaggagtga taaagccgaa
     5461 gcccttatcg ctgttgaacc acttcacggt tccctctgtc atttacttct ctttctttca
     5521 cactggatgc aatccgtgca tccaagaatc ggtggtacgg cttctgaccg cgtcgaaggt
     5581 cgagaacgta acggctagtt gcgttctcaa ctcgaagagc tggatgcccg gggcgcccgc
     5641 gcttcgttcc ccgctgaccg ccagcgctct tgcgcgtcgg ttgcctgcgt gcggatctcc
     5701 ctttcacgcg cgctgaccat gaggaggctg tcaaccgtcg agtcgttgct cggagccgcc
     5761 gcagccatca gagtggcatg cgccttgtcc gggtgggcgt tcggaggcac caccagcaac
     5821 acaatcttgt tgcggttgag tccgagaact tcgacagtgt tcgccggctg aagccggtat
     5881 ccgtcgagcc gtacccgtcg cccgccagtc gcgaaatccg cggatggtat cgcccattca
     5941 ttgcggttga agatcacgcg gtcaacgggc tcgagccgaa ccgacagcac cgccaacaga
     6001 tctggcagct cggtggcgag atcgtcactg cgcggccacc atgccccatc gacatacccg
     6061 ctctgagacg ccttgggctt caagcgcaat cgcagcgtgt gcgccggcgg tgggttgcgt
     6121 tgcccaacat cggtgcgatc ttgtactagc gtcatggtga cgctcccgcc ttgaccgcga
     6181 accggccaga tcctacgaat cggcaatgac acaaccttga acggtcatgt aacgaagtac
     6241 cgccgatatc cctttagtct actcgggttg tccagcccgg agccgcgcgc tgcaacacga
     6301 gacacgacca tcgacgtatg ccgctcgaaa ccgttggaac acaggcgatt ccaaccggtt
     6361 gccgccttag gctcgcggtg ggccgtccgg cggtgtcagg gtggcggtca gaccgaagag
     6421 caggatatcg aggccgcggt cgagctccgc ggcgccgtcg tagttggcca gggccgatgc
     6481 cagcgcgcgc acctggggga actcggtcac cggcaaccgg tgcaggccta ggcgcaatac
     6541 atcgtccgtc tcctcaggtc gctcgatgac ttcctgaatc tcggtgagta tgtggccatg
     6601 gaggtacccg aagagcaccc ggtagatgtg cagcgcatct gccccgctga agccggcgga
     6661 ggtgagcagc gtgagtacat cctccaacgg ccgcagcatc ccgagcggcc gctggcctaa
     6721 cggcgtggat agaggtcgcg tcaccaacag tgacaccacg tttgggtgtg cgagggccaa
     6781 ccgccggaag tcgtgggcca ccgcacgcag ctggcccatc cagtccggat ctacggtgtt
     6841 cacccgtagt tgtccgagca cgatctcggt gacaccgtcg agtaatgccg ccttgttcgg
     6901 cacatgccga tagatcaccg tgggatcccg gcgcacctcg tcgctgaggc ggcgcaagga
     6961 caagccgtcg actccatcgc ggtccacgat cctcaatgcg gattgcagga tcccctgcct
     7021 gctcacctgg ctgtcgtcgt ctcgggcggt ggccacggtg gccgcatcgc ttccggcggc
     7081 gactgcaccg cgcgcggcgt gctgtttcgt catggcgggc accttcccct cggcttggtc
     7141 aagtcggata catcgtatgt taacgccata cgtcaacatt gttgacaaag tcgcgaatcc
     7201 ggtgtgtact gaaggtctac tagcagatga gagacctcga atgctcgtca tcgtcgaatt
     7261 gattggctcg tcccaatcgt ggacgcgctg aacaacgctc gggcgactca cccgctgacc
     7321 gattttccgt ggacggctat cagccctcag gggcgggccg cacgttgttc ctctttggga
     7381 tcgcggtcgg cgcggtgacg tcgctcccac ttcgcctact gttgacgaac ctgtggccct
     7441 ccgctagtcg cgccgccgac gcccgacgtg cggtcgctcg ccgtgatcac gaaacggcgt
     7501 tcatcaacca agaccgcgac accaaactcg aacaccagca acgagccgac acgacctccg
     7561 gcaacgccct cacccaagcg ctacgaatct aaggggccga catggctacc aacacaatgg
     7621 ttttgatcat cgtcaccaca ctggcggtac tggtgctcgc cggcgtgttc gcgggtgtgg
     7681 tgtacaagac acgcaccccg aggtggggtg agatggtccg cgatcaggtt gaggaggacg
     7741 cgctacgact tcggcggcaa cagacgctcg ccgacgagtt cgatgcgagg gcccatgcag
     7801 cccaggtcga gatcgacatc aaaacggtcc gggcgtccag cctgcaacac caagcgacgg
     7861 cctatcgcag cgaagcagtc acctccggcg accaactgaa cgagatgagg ggcggcgcga
     7921 tgaggggcag cgcggacaaa ctgactgcag ccgcacagcc acccaccgtg ccccgactca
     7981 ctggatgacg tctgttacct actcggtcgg tctgtgccgg gcgcgggcgc ggcgcacggc
     8041 ttcgtcgacg atttgttgag ccacgtcagc caacttgcgg tgttccgttt ggctcatcgc
     8101 ccgcaggtgg gcgaacgcgt cctcgacgct gcgcccggta cgtccccgga tgagtccgat
     8161 cgcctgatcg attaccggac gcgtcgacag cgccgtctgc aactgggcgg tgagagacag
     8221 cgcgtcggcg aggatctgcg cgttgtgtgc gccgacggtg gcgccccaac gggtaacgtc
     8281 gtgtaatcgg gaccaaccag gcccctggaa cgtcttccac caaggcattg tcaatgacaa
     8341 gtaccgctga gacgcaacag ctttcgctgg gcgtgatggc tcgttcgcgc aaaccgaacg
     8401 agcgcaggtt gccgattcac ccggctcatt tcagtaggat tgatcgtcgg gtccggccgc
     8461 ggatctttct tgagcatggc tacggctcgg actttggcgc cacggacgtc gcattggccc
     8521 cactcgtcgg tggaatcggt tctcgagaac agctcatcgc cgactgcgac ataattttgt
     8581 tgcccaaggt ccagtccgag gatctatcgg aactcaatgt cggacagatc gtctggggct
     8641 ggccacactg cgtgcaggac accgcgctga cgcagatagc cattgatcaa cggctgacgt
     8701 tgatcgcctt cgaggcgatg aaccactggc aggccgacgg cggattcggg cttcacgtct
     8761 ttcacaagaa caacgagctc gccgggtact gctccgtcct gcacgccatg caattggccg
     8821 gcatcaccgg cagctacggc cggcgcctgc gcgctgcggt catcgggttc ggcgccaccg
     8881 cccgcggcgc ggtcaccgcg ttgaacgccc acggcgttga cgatgtgcgc gtgctgacca
     8941 atcgcgaggt agccgctgtc ggttcgccga ttcactccac ccagattgtg cagatggggc
     9001 aggacgagga ggcgcccgag cggacttggg ccgatacgcc cgaggacggt gttatcccgg
     9061 tggcccggtt tctggccgac aacgacatcg tcgtcaactg cgtactgcag gatcccgcgg
     9121 cgcccctgat cttcgtgaca gacaacgatc ttcagtcctt cacgccaggg agcctgatcg
     9181 tcgacgtctc ctgcgatgtg ggcatgggct tcagctgggc gcatccgacc tcgttcaccc
     9241 agccaatcat cgagctggcg aacggcgtgc actattacgc cgtggaccac agcccgtcct
     9301 acctgtggaa ctcggcgacc tgggagatca gcgaggcgtt gttgccgcac atcgaaacgg
     9361 ttatggccgg tccggttatg tggaactcga ccccgacgat ttcccgggct atcgagatcc
     9421 gcgacggcgt cgttctcaat cccagcattc tgtcgtttca gcaccgcgac gacgactacc
     9481 cgcaccttgt ccgcgccgaa cagtcgcagt aactacgccc tccgctgggc gccaccttcg
     9541 gaatcccatg tcgactgggc cgcagccatt tcattccgcc gagtcctgcg gcggacaccc
     9601 gcccggagta cgtccttgac ggtcaacggg aggtaagggg ccgtcgggtt cgacccgccc
     9661 atctcgccgc ccatctcggc atggttcccg ataacacgtg gttcggtgat gtgcgtcaag
     9721 gttgtcccgc tatccatccg catcggcggc cgcgtggcga atcgtcgcgc gcctgagtcg
     9781 gcggctattc atcggttcgc gcgccgactt ctggtgttcc agttgccgaa tcagctcttg
     9841 cttggcctca gtgagggcgc gggtgatgtg cgcgctctca gcgacggcga tcagctgcgg
     9901 aaggccgggc agcgtagtac gcaatgtgac gcgttgctcc ttgccgccgc gatcctgcaa
     9961 agtcacgtcg acgtccacgt cgcgcgggtt ccacctgccc agatgggacg cgagcgtgga
    10021 taacgccgcc agcacatgcg gacgttcctt tgccgcgaat cccgcgccga cgtggattac
    10081 gttctcgcgg aaaatggttc gttgtttgtc gccagatttg ctcatcgtgc cctcatttcg
    10141 ggctgttgtt cagcgaatct tttcgggaat cgaattcagc gcatgcaaga cgtgcacggc
    10201 gagctcgcct gcagccttgt ccccaagctt cgaatgagtt gtgatggtgt ccttgaccag
    10261 ttcaagacgt aggtcgtagg gcgtacggaa cgttgttggc gaagtcatcg aatttccttt
    10321 tcaatcggac attttcgctc gcaaccgagc tgcccgataa ctcctacggt acgccctttc
    10381 ccgtcgccgc gtttagctac ggggaggcgc tcaaccgatc gacaccagtt ggtcgatcga
    10441 gtccctcgga agatcgccgt cgacgaccgc cgtcacctcc atcttgctaa gcgtgatcgc
    10501 tcccttgtgg ttgtcgagga aggcgatatc tgcggcatcg cggccggtgg cgatgcgcac
    10561 cagtgtctgc cgcggcgcca acaaggtccc gtcaaccacc cgccactgcc cgtcgacgaa
    10621 cgcctcggcc accgcgtgga agtccatcgg agacagtccc ggcgcgtaca ccgagacgac
    10681 ccgggccggc acgttcacgg cccgcaacaa cgccaccacc agatgcgcga agtcgcggca
    10741 gactcccgca ccggcaagaa gggtgtcgac cgcgccgtcg atcgggtcgc tggatcccgg
    10801 cacatagttc agccgggtcc cgacccacga ggacaccttc gccaacagcg ttgtcgaatc
    10861 gagatagttg ccgaactccg ttgcagcgaa accgaagaac ttgtccgcct cggcgtaacg
    10921 gctcgggcgc aggtacatcg acaggtcata ctcggtcaca ggtgccgggc ctgtccgccc
    10981 gacgatcgcg gcgctgtact ccaccttcag attgccgacc ggcacgtcca acttgtgaat
    11041 gcggttgccg tgcgtaccgc tgatctccaa cggctcgaca ctcacgccgt ccaagacgaa
    11101 cgacagtgcc tcggacaccg acgtattggg atgcggtgcg accgcgatct ggaactccaa
    11161 cgtcgtcggc gccgtaatct cgacctcgag ttcagcgcca acctctcttc tcatctgtct
    11221 tccctcttca ccgatagtcc acgagtgcgg ccagcagctc acccagcggc acagtcgcga
    11281 ctccgattgc ggcgtcgccg attccgtacg gcaccactaa tgtatcgccg tgcaccagcg
    11341 caccgcacga gtagacgaca ttggggacgt aaccgtcctg ctcgtcaact gcggggctca
    11401 gcagcggctg gtgaagctga ccgagaatcc gtgccggatc gtcgagatcg agcagcatcg
    11461 ccccgattcg gtacgtccgc atcgggccga cgccgtgggt gagcaccaac cacccggcct
    11521 cggtttcgat gggcggaccg cagttgccga gttgcaacgc ctcccacgct cgagccggct
    11581 gctggcatgg aaccgattcc gtccactcga acggatgatc cgaatatgca atcgaattcg
    11641 attcgcggtc cgatcggcac atcgccgcga aacggccgcg gatccggcgg ggaaacagtg
    11701 cgagaccctt attggccgcg gcagggccca ccatcggcgt ggacgtgaac atgcggaaat
    11761 cttccgtgtc cagcagctgc tggctgatgt gggacccgct gtaggcggta tagctggcat
    11821 agaacgtgac cgaaccgtcg tcgtcgacga aacggacgaa tcgcgcgtcc tccatgccgg
    11881 cgccctccgc gctcatcgcc ggccacaaca cccgctcgga aagcgggatg ccatcgtcga
    11941 attcgacacc gtaggtccgg tcggcgatgg ttcggatcaa cgaaatcgtc tcggccgcac
    12001 ggccacgcgt ccgtcggtgg gtcagaagct tgtcgacttg ttcgtcgagg tcggcgcggg
    12061 tgaactggtc gccgagcgca tcgaggacgt aatcggcggc ctcgccggcc ccgtgaagcc
    12121 gacgcagctc gctgcgcatc accgcaccgt ccaacagcgt cggcacgact ggtccgacgc
    12181 tggcaagcgg agccggatcg tcgattgtga cgccgtctgc cccgtcgacg acgccggtgc
    12241 ggaacccgat ggacgaacgg tgcccctccc cgatgccccg cacgctcatc acgaatcgca
    12301 ggctgccctc gggggttccc gcctggtccg ggtgcgccac catgctgggg ttgcacaacg
    12361 ccgcgccctc aatcgcgtac tcgttggtga ataccgcacc cagcaacaac attcgcgcat
    12421 cggaaagatc agaggcggga tccacacgat cggcgacttc acgggcgtgc ctgcggaatg
    12481 taccgaccag gtcccggtgg cgcccgtcga agcgggcgac gacatcgtcc agagaggccc
    12541 gtacctcgtc ctcgtcgagg gccaggacgc gagcaagcac cgcgcccgcg cgggaatcct
    12601 ggtgttcgaa accctcctgc ccgggcacaa acagccgcgt gatgactctg gaccggtcgg
    12661 ccgctagccg ctgcgggctc cgcgtgacca gcccgacctg caaggaagtc attgccgcac
    12721 agtcgataaa cgtcgcgcgt gctgcaaggt agagatcacc gccaaggtcg actccgcgcc
    12781 ctggttattg tttaccccat cggcatgcag tccgtcgtat ccgccaccgc tggccgggtc
    12841 ccacatgggc tcacccgcat cgttgtcgcc catgaaccac gccgctgccg cctcgacgcc
    12901 gtcgcgccag atcgggttga tgtcgacggc cgcggcacgc gcgcacgcat cggcgagtga
    12961 ggacacttcg atgggctgct gatcgaatcc gggcctggca tcgtcggggc ctctgcccgc
    13021 gacaggggtg ggcgacagat ggccgtgcgc cgtttcgaat tcgacgagcc acgccagcag
    13081 gtccaggccg cgctgacaca acgtcgcgtc ggcgagtgcg tcaccggcag cgatcatcgc
    13141 ctcgggcaga acggcattcg cgtacgtgag tcgcggctcg ggccacggcc acgacgagtc
    13201 ggtgttcgga gcgggcaccg ctgcggcata gtcggtgagc agtgcgcgag ccgggcggtg
    13261 ctccgggtcg aaggcgagga gttcggcagc gcccagtgcc gcgaacgcca tcgccctcgg
    13321 ccatggcgac cgctcctggg cggcgcgctc gaactgcacg atggccgact ggcgggccca
    13381 cacgacgtta ctgtgcgcgg ccgcggttcc gagaccccag atgcaccgcc cccaggcgtc
    13441 ctccatagtc ggctcatcga cccagttgcc cgtgctgtcc atccgattac ggcagccgcc
    13501 gtcaagagcc tgcgcatcgt tcaggaatcg caacgagact ccggccagac cgttcagcgt
    13561 gcggtcgggc ccatggtctc gggtggcgac aacaagcact cgcgctacat cgtcggtgca
    13621 atagccgtgc tcgggcagcg gctcggccag ccgagcgtgc tccaaagtgc cgcgacggtc
    13681 cgtcagccgc agcagatggt cgaataccgg agccgggacc gtcgcggtca tgcccatgcc
    13741 cgccgctgtg cgagaacctg atgcgccagc ctcagatacg cacttccgac aaccggccac
    13801 gccatctccg gcgccagccg gcgcgcctcg gccgccatcg caccagcgag acgcggctgc
    13861 gtcagcaccc ggcgcagagc ggatgccatc gcgtccgggt cgtcatggcc gacgacgatg
    13921 ccggccccag tgccgagcag ctcgacggcg tgcgggaacg cggtggccac cacgggccgg
    13981 ccgctcgcga tggcgtcgac cagaactccg gaggtgacct gatcggtcga gtcgtacggc
    14041 aggacgacga ccgccgcgga ctggatgagg tcagtcagca tcgcaacgct gcggtaaccc
    14101 gtatcgaagg acaccgaatc cgcgacaccc ctgcgccggg cctgttccat ccgggcatcg
    14161 cggtacgcct cgccgtctgc ggccagcacc ttcgggtgag tttggccggc aaccaggtag
    14221 cgcggccggc cggcgacatc gctgagcgac gccatcgcct cgatcacccg ctcgatgccc
    14281 ttgcccgggc ccagcaggcc ccaggtcaac acggtaggtc gaccgcggcg cacggtggag
    14341 gcagtcgtcg ggatggttgc accgtgcgga atggtggcga ctttgcggcg gtcgatgtcg
    14401 aagcctcggc ttagccgttg gcgggccgcc tcggacatca cgatcacctg atcggcccgt
    14461 gctgcgagcg cctcgaggac tgaacgctgg tgtcgcgtag ggtctttgag gatagtgtgc
    14521 gcaacgacga tcgacggaac gcgcagcccg tccatgacgt cgaggacttc gacgccgtcg
    14581 acgcctccat agacgccgta ctcatgttgg atgaccgcga cgtcactctg gttcagcaac
    14641 tcggcgcacg ccgctacaga cgtcggtgag ccgttgacca tttctccgat gacacgggca
    14701 ctgtccgacg gcggcccgtc ggagactcgc acgacgctta cgtcggcgcc attggcggtc
    14761 aatccccgtg ccagcgcagc gctgaatgtc gccagcccac acgctgtcgg tggatacgta
    14821 ctcaaaatgc tgaaacgcgg agacccggaa aatccatgac tcacaacggg atacgtcgag
    14881 ggaatagtag acgtgctcaa ggtgatccca acttgcggtt gctacctgcg tgtccggcgt
    14941 gagcggttgc tcggcgtggt cagcggcgac acggctgact catcccggga ctagctgggt
    15001 tcccaacttc ttagacgata cacccgaagg tcgatcgcga cgtaacgggg cgcaaatgcc
    15061 cgtcgtgccg tggcggcata cagtcgaact tcacatcacc gaatgctgtt gtgcctcaat
    15121 ccttcttggg ttgaatggtt ttcggggcta tcggagaaaa cgggagcttt caccggttcc
    15181 gaactgcgcg cagtcttcga cgctacttcg ggctcgtagg gatgcgaaaa cagattagat
    15241 gtatccatgt ggtaggtcct cagattaagc ccgtaggtct gacttatcgg gcgaaagcgc
    15301 gatcgattcg cgggcttacc taccaatcta cacgagtgtc agaacacgac cgcccagcgg
    15361 cgaccgtcaa cgccagaaac ccttcttgaa ccgcttctgg tcgccttcga cgtcggtcca
    15421 ctcgccggac gcgtagctga aggggtgctt ggcgccggtg cggacgacgt cgagtgtgcc
    15481 gtcgtggttc ttgacgtagg cgtccccgaa ccgcacgtag ttgtcggttc cgccatcggg
    15541 gagtgtcacg gtaaccgtca tggtcgaaac ctccggacgc gccggcgaaa tgtcgggcgg
    15601 tggtagccgt tgggcggcgc ttcttcttgt tcctgcgcgt cgatcccgag caggtcgcca
    15661 accgtcgtag cgtcgtccgg cgccgccgcg gtcatcatcg cctcgtgtgc aatgtccgga
    15721 tcggtgtgcg gaggaaccac cagcagaagg agcttctcgc ggccaacccc cagcacctgg
    15781 agggtgttgg ctggttgccg ggtgtacccg tccagtcgaa ccgctcgccc gcccgtcata
    15841 agtcgcttcg gcatctgcgc ccattcaccg aggttgtaca gcacccgatc gatggcgccc
    15901 aggcgcaccg acagcacagc cagcaggtcc ggcagctcat tcggaaggtc atcactgcgc
    15961 ggccaccacg ccccgtcgac gtgcccggac ttccgcgcct tgggcttcaa ccggagccgc
    16021 ggtgtgctct ccggtcctgt ctgccgcccg ccggggaccg agccgccttc atgtcgggtc
    16081 atacgacgac cccttcttgg ccgcgaaacg gccaattcac tcaagtcggc gacgacgcga
    16141 tctcagatgg tcgcgcacga catatcgccg actctgttca cattgcatcc cgaacggacg
    16201 cgaatgctag ctctcgatgt gagagctgtt gcgtctcaat gcttcttgat gtgcaggatg
    16261 tcggtcgtcg acgacgagcg ctcagccttg gcgcgcttga ctgctcgctt ttctttcagc
    16321 gacttgccag atttcttagt catcgtttgt cggggagatt tatcggccac actgacccct
    16381 agatacttgg gggcctgaat ggtcccaatg ccttgagcgt acgcctaccg gtcggatccg
    16441 gcccaaggac tcccccaaac gcattgtggg ctggcgggaa acgacccacc agcccacatg
    16501 aggtacagcg tgttagacgg cagtcactcc gactgcctgc gggcccttgg ccccctgctc
    16561 gattgtgaac tcaactcgct ggttctcctc gagcgactta aagccgttgc cctggatttc
    16621 tgagtagtga acgaacacgt ctgcgccgtc gccgtcagga gcgataaaac cgaagccctt
    16681 atcgccgtta aaccatttca cagttccctg tgccatactt tccaacttct ttcattctga
    16741 acggatgccg acaggcatcc cagccaagcc tagcatggaa gtgcgtaaaa acgtcttcga
    16801 ttggggcaga atacttttcg tgcgctagcc gagccggccg ccagctcgca ctacgtccat
    16861 aaacccctcg gtgttaatgt caacggcgaa ccgtttcgta gtcagcggtg atcctcacga
    16921 tcaacgtgcg atccgattcg taagccggcc tgggcacccg tagccggcct cgaagtggag
    16981 gaaccgtcca tggatcacac gagcttctac gtcgatcttc ctgcaattca agcgatttcg
    17041 gcatgacgag ggagatacac gcgcggcggt tgagcagtcc ggtccggttc accctggcgt
    17101 ccaacctcgg cagcggcctg gacggagcct ggtggccaca caccgtctcg atagcacgcg
    17161 agctttccgg cctggccgat gccgtgcgaa atccactggg ccaagtgatc gatatcgggg
    17221 tcaactggtc gccattgcaa ggtgtccccg acctcgactt gctcaatcgc cgtggtgtcg
    17281 cggcaacgcc cggtcgggac agccgtcatc ttcgcgtcat gacgatcacc ggcagccgcg
    17341 ccaaagcgca tctactcgtg gtgccctgcg ggacaagtat cgccctggct gtcatgctgc
    17401 tgcgtcaagc cgccgacctt ccggttctgt acgaacacca acacacctcg gctttcgaaa
    17461 ccgccggtgt catcgtgcgg aacgcgcgcg cccaaaactc accaagcgcc gcacctgcgg
    17521 agacgtcagg ctgacgcact ccgccggcgg ctatgtccgg tgtgtgtgga actggcggca
    17581 cttgccccgc ctctcgggcg ccgggcactt gacgagcgac gaccgcgcga cccgcccgtc
    17641 ttcgtgttgg ccggtttcgg agtctggtca gccagcacag cgggagcctg aaacggcgcg
    17701 acgtcgccga ccagttcggt caccggggtc gaatcagctt cgacctgttg cggacgcacg
    17761 ttgatgcccg ctctgcgcat cagtacctga acgtccttgc gctgttcggg aagcaccacg
    17821 gtgacgacgt cgcccgcgtt gcccgcccgt gcggtccggc ccgagcggtg caggtaggcc
    17881 ttgtgctcca tcggcggatc cacatggacg accagctcga catcgtcgac gtggacaccg
    17941 cgggcggcga tgtcggtggc caccaacacc cgcgccacac cggtgctgaa ggccgcgagg
    18001 ttgcggtcgc gcgccggctg agaaaggttg ccgtgcaagt caactgacgg caccccggat
    18061 tcggtgagag tcttcgctag cttgcgcgcc tggtgcttgg tgcgcatgaa caggatccga
    18121 cggccgctgc cggaggcaag cgtgtgcacc aattccttct tggcctggac tcccgcgaca
    18181 tggaacacgt ggtgcgtcat ctcggccacg ggtgagtgcg cctcgtcgac cgagtgcgtc
    18241 accgggtttc gcaggaatcg cttgaccagc ttgtcgactc cgttgtccag agtcgcggag
    18301 aacagcagcc gctgcccgcc ttgcggcgtg gcggccaaga tgcgagtaac gcctggtaag
    18361 aagcccaggt cagccatgtg atccgcttcg tcgacgacgg tgacgtcgat ggtgtcgagg
    18421 ctgatgacgc gctgcttcat caggtcttcc agccgaccgg ggcaggccac gacgatgtcg
    18481 acaccggacc gcagcgcagc cacctgccgg ttctgcggga cgccgccgaa gatggtggtg
    18541 accttgaggt ggtatgcggc cgctatcggc tcgagggtgg ccgcgatctg cgaagccaat
    18601 tcgcgggtgg gcgcgaggac caatccggtc ggtcgcgacc ggcgcttcat cgctgccagc
    18661 cgcgtgatga gcgggatcga aaacgcgagc gtctttccgc taccagtctt gccgcggccc
    18721 aacacatctc gtcctgcgag accgtcggga agcgtctttt cctgaatcgg gaaaggagtg
    18781 aggattccgc gcgcggcgag cgccttgacg agcggctcgc cgacgccgag atcggcgaag
    18841 gattgagttg tagtcacgta gaggagtgcc tttcaggcat cgggttgtac tagacgagca
    18901 agcacaaggg tggcgagatt gccggtgggc aaaatcgatc gccgcgagaa gtgcttgtgc
    18961 tgggggcacg gatccggagg gatggaagcg ttccacgacg gggttggcat cgatttcggt
    19021 ttgacgccaa cgttgttgca cagcttacag gcacgggcgt cgtcagctcg caaaatccca
    19081 ggtcgccagc acgtcaagct gccagacgtc gcgacaatgc cggacggaag tggtatagcc
    19141 agaaggcttt tgacgatcgc aacccggccc gcgatcgccg gatgtgatag cgatcactaa
    19201 ttagtagagc acgtgttcaa cacctcggcg gccacgggcc ccgaccaccc cgacggacgc
    19261 gttcgaggca ccagtcacca cggccgcccc gcttcgctgc gtacatcgtc cggtctgcgt
    19321 cgcgcagtat gtcctccgcg gttcgctcgt cgtcgcggtg cacctccacc agcccgacgc
    19381 tggcgcggat cggggtggtg gtatcggcca gcacgaccgg ctcagcgagc cgaagccgta
    19441 gccgatccac cagggagtcg atttcgccct gcgtctccgc gccgaagatc agcacaacga
    19501 actcgtcgcc accgtgccgg cccaccacgt cggccgggcc gacggcctgc cgaagtcgtg
    19561 ccccggctgc gcgcaggagg tcgtcgccgg cgtcgtgccc caacgtgtcg ttcgtcgtct
    19621 tgaggtcgtc gagatcgatg aacaaaaccg cacgcaaccg cccgtccgtc gccgcgagcg
    19681 cctccgcgat tctgcgcagc acgaatccac ggttcggcaa gcccgtcagc ggatcatggt
    19741 tgaccttgtg aaccagccga tcgacctccg cacgctctgc ggtgatgtcg gaaaacgaaa
    19801 agagcatgtc caagtccccg ggggcatcgg ggttgagcag acgcccgctg gccaccatcc
    19861 acttccgctc gccgttgggt agctccgttc cgtacacccg cctcgagaac gtcaccccgg
    19921 tgcggaacaa ctgcgccacc ggtctcagct ggggcggcac cggttcgccg tcggcgtcat
    19981 agatcggcaa cgcggccgtc cacctcgcga agtccgcagc cttgtgctct ggcccgagac
    20041 cgacgatccg catagcggcc gcattgacgt acttgatgta gccgtctttc cgcaatacga
    20101 cgaccccgtc gtccagcagc tcaacgagcg catgcatttg gtggacggcg cgacgcggat
    20161 cggtcagacc gtggtcggtg tggtggttgt ccacctacgg ccttcctttc tcgcgatggc
    20221 gtcatcgacc agtcgacgcg gacggtttca cgagccaggc gtgcaccttg ctgtgactat
    20281 ttcagcacaa ccaagtgatt gattgttgaa caaatcagat gccggtgtgt ccaccgacca
    20341 ggacgaccaa gtccttgcaa ggttgcctgt ttacagtggc aaagcggtgg tccgtgcgct
    20401 gtttgcgggc caccgcacgc gaaggtcgag ttctagcaaa aattatcgcg acagcaaaat
    20461 gcgacacgtt tgcgaaatca gcgctttccg gccaacttcc gctactctca gctgcgacac
    20521 caccgggggc ggatcgaggt gagcgtgaca tcaatgctcc gcgccgtgtc cagccccctg
    20581 cgaccgtcga gtcctctact gcgggtgaca cgtccgtgac agtccaacct cgtctcgtgc
    20641 cgcctctggc gcaaggcccg cacctgccgc ggcacggcgc cgcgcacgca agtgtggtcc
    20701 gcgagcttgg gtttcgacct gaccagggca ttgacgccgc gtcggccggt gtcggtctga
    20761 cgccctggtt tcaaccggtg gtgtcgttac ccggtgaaca ggtcgttggt ttcgaagctc
    20821 tggcacggtg gccgactttc gtcacgatga cggcccacaa cgtgttctcg ttcgcgaacc
    20881 gctcgcggca cgccgatgtc ctcgatcaac agtgcatcga tgcggcggtc catgccgcgc
    20941 tcgattcgga gctgccgcgc gacagcctgc tactgatcaa caccgaaccc gctgtcgcac
    21001 acgtcccctt agccaagaat ccggcgctga ccgaagcggg tgaacgattt cagctggcct
    21061 tcgaactcac cgaacgccac atgctcgcgc atccacagac gctgctggag aaagtcgccg
    21121 cgatccgcgc tgacgggatc gcgatcgccg tcgacgacgt tggcgcgcat ccggattcac
    21181 tcgcggtgct cgacatcatc gaccccgaca tcatcaagct cgacatgacg atgatccaaa
    21241 actgcacaat gcgaaacaag gccgacgccc tgaccggtgt ccttgcccat cacgaacgga
    21301 ccggcgcgct gatcatcgcc gaaggcgtcg agactgacga agatctcgag caggcgctcg
    21361 cgatgggggc cggcttcgcg cagggttatc gcttcggccg agcccgcccg ctgtgccggc
    21421 agccgctccc ggcaggtcga ccgccggcgg tgcgtgcaca gcggcaccct cgcagttctg
    21481 cccacgccgc agcggaggac ggccggaggc cgctgcgtgt ggcgcgccag cacgttgttg
    21541 gcgcattcac ccgccacatc gaggagcagg cgcggcactc cgtcgatcaa ccgatcgtgt
    21601 tggcggcatt gcatcgagca cacgacttct ctgccgcttc gcgacagctc taccaggaac
    21661 tggcggagac gtcgccgctc gtcgtggtgc tcggccgcga catgcccgcc gacctcggcg
    21721 gaggggtgcg cagcgtggag ttgcaacccg aagatccgct gtgccgggag tgggtcgtcg
    21781 tcgcgctcgg cgcggatacg tgtaaggcat tggtcgcccg cgaactcgcc gataccgcaa
    21841 aacacaacgg cgaccggcga ttcgaattcc tcgtcaccag cgaccgtgca ttggtggcga
    21901 aggccgcgcg aaacctgttg gctcgcgtgg cgtgaactcc accggcaacg ctgccggtga
    21961 gcggaacgcg acgccgttga taaccggcgc tggtgcactc ggatccaggt gcaggtccgg
    22021 cagtcgatcc aacagcgcgt tgatcgcgac ggtggtctcc atccgcgcca gcggcatccc
    22081 cagacagaca tgggggccgc cgccaaacgt cagatgcgcg aagttcttgc gcgtcgggtc
    22141 gaaccggtcc ggatccggat agtgcgccgg gtcccgattg gccgccgcga cactgacgct
    22201 gacattggtg cccttcggaa ttgtgatccc gccgagttcg cagtcttggc gcgcgacccg
    22261 caccaccgta ctgatcggcg gctcccatcg cagcgcctct tcgatcgcac cccggagcat
    22321 ggagcggtcc gcgcgcacgg tgtcgagcag tgacggctca gtcagcatcg ccaccaacag
    22381 atttcccgac gcgcggtacg tcgtctccac tcctgccggc aggatcaaca acagaaacgc
    22441 gaaaatctcg tcatcgctca gccgttggcc gtcgatctcg gattcggcga gcgtgctgat
    22501 caagtcgtcc tgcggatcct ttcgacgctc ggccaggatc tcaccgaagt actccttcaa
    22561 cgtcgccgac gcccccatcc cgacgtccca gtcgtacacc acgctcagca gctcaatcga
    22621 caactgctga aaccgcacat agtcctcccg cggcagcccc atcagccgcg cgatcacctg
    22681 cacagggaac gcaaacgtga actcccgcac gagatctgcc ttgcctcgcg gcgcgaagcg
    22741 gtcgatcagc tcatgcacca ccacctcgac cagctcggtg cgccagcgct ccagcagctt
    22801 cgccctgaac aacggcgaaa ccaacgccct gctcgcccgg tgctcgtctc cctcctgttg
    22861 caacagggtc ttccccatca cagggcccat gatgtgctcg tagatcgacg acgagaagtt
    22921 ctctggatga gtgagcaccg tttggcactc gtcgtagccg agcaccgtca ccccggccat
    22981 gtcgaggttc tgcgccggcg tctcgacgat tggattgccg accatcacgc gatgcttctc
    23041 ccgtgccacg cgcagcttct cgtgcacgtt ctcgacggca ctcagaagtc cgccctgcaa
    23101 cttgccgggg tcgaactcct ccagcggcgc ccggttcgtc acgacgccgt caccgtctcc
    23161 gtgtcacggc ccgaccgcag cactgtgccg ggcattgcgc ccgttgccac cccgtcgcgg
    23221 cacacctcga caccgcccac ccacacgccc gtgatcccct tcgcgtcggc caccaaacgc
    23281 ggtgcaccag cgggcaagtc gtagcgtgtg cgttctgggc cgtggtcgac cgtcgtcggg
    23341 tcgaacatca ccaggtccgc gtgccagcct tctgcgatgc ggccacgttc cttcaggccg
    23401 tacagccgcg ccggcacgtc ggtgatgagc cggaccgcct cctcgagcgt caccacctgg
    23461 tgctcgcgca cgccgtggcc gagcagcgac gtcgtgtaga tcgcgccgca catcatgtcg
    23521 agatgcgcgc cggcgtccga gccgccgatc accgcgtccc ggttgcgcca gacttgggcc
    23581 cgcgctttcc agtccgcctc gtcatcgccg aacggcctcg gcgacagtcc ggtgcgcaac
    23641 tcgtcggcaa tcacgatgtc gagcagggtg tcaaacggtt ctttcccacg ggccttagct
    23701 acttcgccga cggtgcggcc cgtcgcgtcg tcgttctccg gcgcgaacgt ctcgacgatg
    23761 atcagccgct cccaattggc aaggccccga aggattcccg cctcctccga atgggcgccc
    23821 tcgttgaggc ggcggcggac ttcgggatcg gcgagcgccc gcagccgctc ggccaccggc
    23881 aggtgcattg tctcgcgcca gcccggcagc ccgtcgagca cgaagcccga caggaacgac
    23941 aaccggatct tcgtcgagtg cggcagggtc agcgcgacga tgcggccacc acgttcggca
    24001 gcgacttccg atgcacgcag ctgcttttcg tgcccttccg ggtttcctgc cgagacgccc
    24061 agcacgttcc aattcaacgg ccggtcggcg gccctcgaca tgtcggtcag cagcgcgatg
    24121 tcatcgtcgg tgaagccaga cagacagccg ggaatgatcg cctcgagctg ggtgcccgga
    24181 tggttgcgca gcacggccgc aagcgccacc agctcctccc gcgacgcgct gcgcgacggc
    24241 accggctgtc ccgagccgtc gttgtgggtg ttcgactgcg atgtcgacaa ccccaacgcg
    24301 ccgtccgcca gcgcggcact aagaatcgtg gccatctttt cgatctgctc tggggacgcc
    24361 ttgccgccga ctgcgtcatc gcccatcgcc gccagccgaa tcgccgtgtg gcccaccagg
    24421 aatccggcgt tgatagcgat gcggccgtcg agccgggaca accaatccct aaacgacgac
    24481 cactgccagt ccagcccagc gcgcaacgcc tcgagcggca tgccttcgac ccgggccagc
    24541 atccgcgtca ggtagtcctg ctggtccgcc acaggggcca gcgtgaagcc gcagttcccg
    24601 ccgaacacgg tcgtcacgcc gtgctgcacg gacgggctcg cggtcggatc ccagaacagc
    24661 tgcgcgtcgt agtgcgtgtg caggtcgacg aagccgggtg cgagcgtctg cccctcagca
    24721 tcaacggttt tcgacgcggg ttcgtcgact tcgccgactg cgacgattct cccatcacgc
    24781 acacccacgt cgccgtggcg ggcgggcgcg ccggtgccgt cgacgatcga ggcgccccgg
    24841 atcaggaggt cgagcatgtc agccacttgt cgtcgggcag cggatgacgg aacagctccg
    24901 cggcgttgcg gtgggtgatg cgggcggcgt cctccgcgga cagcttcgcg acgttgcgcg
    24961 cgacgacgtg ctgcgtgtcg ggccacgtcg agtcggcgtg cgggtagtcg ctttcgatca
    25021 ggatgcgctc ggccccgatc gcgtcgagcg cgccgaacgc cgacgggtcg tcgatcgagc
    25081 agaaccagaa gttgcgccgc agcacctcgc tcggcagcag gtcgcccttc caggaaccgc
    25141 cctcggcgcc cgacgcggag tgggcgagga cgaagtcggc gcggtcggcg agcatcgccg
    25201 cccagccgag gccaccctcg tcgagcgtga tgttcagctt cgggaagcgg accgggatgc
    25261 ccgaccacag ccaatccgcg catgcgacaa gggcattgac ggggaacagc gtcgtgatgg
    25321 tctcgaacgg ggtgtccggc gcggggatcg gcgcccactg cgcggagccg gtgtgcagac
    25381 agatgacggt ttcggtctcc tcgcacgccg ccaggaacgg atcccacacc ccggtgtgca
    25441 ggctgggcag cccgcactgg gccggcagct cggggaagct gacggccttg aagccgcgat
    25501 cggcgttgcg acggagctcg tcggcggcca gctgcgggtc ggccagccag ggcagctgca
    25561 gcgggatgat ccggtcgggg taggggccgg cccacacctc gaggtgccag tcgttccacg
    25621 cccgaagcac cgcaagcccc aactcgggat cgtcgctctt ccagaag
//
LOCUS       DASRJA010000232         7496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2032113,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000232 DASRJA010000000
VERSION     DASRJA010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032113"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VFC01_10535"
     CDS             complement(<1..841)
                     /locus_tag="VFC01_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZN80109.1"
                     /translation="MTSATAEVPSGIDDVTPQWLTTVLGGATVKDLRAEQIAQDTGFS
                     SLLYRLHLTGDVGVPPTVIVKLPAESEARGAMDLLGGYRREVAFYRSVAGNAPLSTPE
                     VYAARMVEGSADFVLVLEDLREWDNADHLAGLSMERARLCIAQLAGLHAWSANSANSA
                     VLEQFPTLDTPMVRDLFLPAFGPGWQVYLEKSSEPVPSEVARYADRFAELAPLALEAL
                     AERSMLLHGDIRADNMFFDGDRLKVVDFQFAARGAGAADIAYLVSQGLPTEVRRSHDK
                     ELVC"
     gene            909..1529
                     /locus_tag="VFC01_10540"
     CDS             909..1529
                     /locus_tag="VFC01_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80110.1"
                     /translation="MPSPARGLTQPERVEASTRRLLQAAAELIVEKGWDAITAAEIGR
                     RAGYSRAMVHARYGSKDAILEAFLHEYVKRLNPDPDPKTSGMQQVLAHFDRIEEIYTE
                     DHELLLAMFVATFEAVKTTSPLRQRVRHQLTSGAAKVEKGLRKGIRDGSVRSGIDIAR
                     TVSDISTSVFGIAFWWTALPDQYDLGRELTNVRTRIVENYGGPALE"
     gene            1702..3117
                     /locus_tag="VFC01_10545"
     CDS             1702..3117
                     /locus_tag="VFC01_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZN80111.1"
                     /translation="MSHTAVVINTAVAIIAVVVMIVRFKFNPVVSLVVGSAYLGLAVG
                     LGVEKTIDAIMTGFGDIMAEVGLLIAFGVLMGAILQQTGAIQRLVQTLLNLFGPKRMP
                     YAMSLTIATLLQSIFLDVLLVISAPLGRSLAKKIGKNGTARMAAAMAIGLECGIVLTV
                     PGVGALALAGLLGVPLGKYLLFGVLLVIPTVAISVAIMSFLFNHGFWNPERDEQEFLG
                     EDPEPLDDERETVSATTTPDDDGSTGGGVATKTATVTRAQTPLIVLFAPMLVSLVLIA
                     TGAILDAGDIHNPIVKFISSPVIALLIGLIGTSFVGRYTLGAEPIQHAIATGFKESGQ
                     ILILTGVGGSLAATIKAAGLGDILGEYFTASTAAPLLVVWAIAAALHVAVGSVTISAI
                     TSAGILAPVAPVIGLDPVLIALAAGAGSLFAVHVTSNTFWLLQSLLGQSTRGALKTVT
                     VGVSVASVVAILLILPMSVLF"
     gene            3137..4345
                     /locus_tag="VFC01_10550"
     CDS             3137..4345
                     /locus_tag="VFC01_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZN80112.1"
                     /translation="MNTTDIRPLAGVRVLELGNYIAAPTAGRMLADFGAEVIKVERPV
                     TGDELRNWRLYSGDTSMLYRTINRNKKSIVLDLKTERGREIVLELVRHCDALLENFRP
                     GTLEKWGLGPEELDRVNPKLVITRISAFGQTGPLSQRPGFAAVAEAFGGLRELVGDPD
                     RPPVRTGVSIGDSIAGIYAAFGTVMALFQRESVKVPLPQRVIDVALNESVLSVMESLV
                     PDYLAYGVTRERVGGRMEGIAPSNAYPCAHGTSIIVAGNGDAIFQRYMQTIGRPDLAD
                     DPDLQTNAGRWRRRDELDAAIGAWTIQRSRDEALKLLDEAGVPSGPIYTAADICADEQ
                     YEARNMVQHFDVDTGEPQPKAVGFPGIVPVIGGASLPIRSIGPDLGEHTHEVLSTVLG
                     LSDDEIGALT"
     gene            4342..5283
                     /locus_tag="VFC01_10555"
     CDS             4342..5283
                     /locus_tag="VFC01_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HZN80113.1"
                     /translation="MTSRPYTPRAELRDVTLRDGLQLTAKMLPTERKVQIVRELLALG
                     VPALEIGSMARPDLVPPLADTLDVIAGLDADELEHCWIWVATPRHVEKAAAAGARNFQ
                     YCFSASDSHNKANIGRSTEDSVAAMPDAVQIAREAGGSIQLCIATAFTCPFEGVTDPE
                     RVIGIAADPRTDGADDIVVCDTIGQAIPTQVTDLIEGLTARSPQRRIVFHGHDTWGLG
                     VANTLAAIDAGATMIDGSLGGLGGCPFAPGASGNTSTEDLLFATRPDWFTPATLASMV
                     DITDKLLAELGEPNRSRTAQGARSKAKAFDWVIDRGA"
     gene            5303..6088
                     /locus_tag="VFC01_10560"
     CDS             5303..6088
                     /locus_tag="VFC01_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015399587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN80114.1"
                     /translation="MATDDSAASSKQQPLLVLGKITEILDAFSLTHPDMSLGEIQQAT
                     GLPTSTVQRLVSNMVAQGLLDRAGDRIRVGVRMSYWAATALKDLDVLAVVNPVLKEIR
                     DKTGETACFFKVEQNFRVCVAVAETHHALRRDMYVGKVIPLHVGSASRILLAWNPDLA
                     SAILAGPLEPMTDGTVTIPDELRRLIAQAKADGYAITAGEREDSASGLSAPVFDAAGD
                     ILGAITISGPTVRMPQAQCEAWIDLLVGYAEQVTRTIGGRLPH"
     gene            complement(6054..7175)
                     /locus_tag="VFC01_10565"
     CDS             complement(6054..7175)
                     /locus_tag="VFC01_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HZN80115.1"
                     /translation="MKTSERAWRIGRDVAGVLPRAYAALRTDGWRPLSSTDLRLLGEV
                     LMDEAALTGMTLTAPPPKLERSVQACQAAADELSELGSQAHADPEPLHVKEIRRQRFG
                     TMVYERLTFEHDPLLPGSLAAGGLAGPATAAVHLCRRDDQPRPWLVWVHGAGQGQPLD
                     LVFSRARRIQNELGVNIALPVQPGHGIRRKAWPPYPNMDPLANVAGMMRAVSEVRAVV
                     RWLRPQTTAIAISGVSMGSPVAALVSHLEPVDAVAVYTPIFGLNAMIAQHLGRWGPSV
                     RDVSDVLGSATVARLASPVDVLATEPTPPPERRLIVGAWYDRMALREPAIALHERWGG
                     ELFWHDGSHAGHLFSRRVQAVSERFLGGLSAEGVRRLSG"
     gene            complement(7179..>7496)
                     /locus_tag="VFC01_10570"
     CDS             complement(7179..>7496)
                     /locus_tag="VFC01_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanone monooxygenase"
                     /protein_id="HZN80116.1"
                     /translation="PSAATNFVAALEQHVEWIGDCIAYLCSHDYQTIEALPDAQREWI
                     EHTTSLVAPTVLVHPTCNSWYNGGNVPGKKRMYMGYTAGIPEYRRRCDEIAAAGYTGF
                     KLA"
BASE COUNT         1339 a         2543 c         2360 g         1254 t
ORIGIN      
        1 cgcagaccag ctccttgtcg tggctgcgac ggacctccgt cggcagcccc tggctcacca
       61 ggtaggcgat gtcggcggca cctgcgccgc gagcggcgaa ctggaaatcg accaccttga
      121 gtcgatcgcc gtcgaagaac atgttgtcgg ccctgatgtc accgtgcagg agcatcgaac
      181 gttcggccaa cgcctcaagg gccaagggtg cgagctcggc gaagcggtcg gcatagcggg
      241 ccacctccga cgggacgggc tctgatgact tctccagata gacctgccag ccaggcccga
      301 acgccgggag gaacaggtcg cggaccatcg gtgtgtcgag agtcggaaac tgctcgagca
      361 cagccgagtt cgcggaattg gcagaccagg cgtgcagccc ggctagctgt gcaatacata
      421 accgggcacg ctccatcgac aggccagcca agtgatcggc gttgtcccac tcacgcaagt
      481 cctcgagcac caggacgaag tccgcagaac cttcaaccat ccgtgcggcg tagacctccg
      541 gcgtgctcaa cggcgcgttg cctgcaacgc ttcgataaaa cgcgacctcg cgtcggtacc
      601 caccgagcag gtccatcgct ccccgggcct ccgactcggc tggcaacttg acgatgacgg
      661 tcggtggcac accgacatca ccagtgaggt gaagccggta cagcagggac gaaaatccgg
      721 tgtcctgtgc gatctgctcg gctcgcaggt ccttgacggt cgcgccgccc agcacggttg
      781 tcagccactg cggggtcacg tcgtcgatgc cggacggaac ctccgcggtg gctgaagtca
      841 cccttgacct cccaaagaaa tttgacgcta aacaactaag ttagttgtcg gccgtctaat
      901 atgcaagggt gccttctcct gcccgcggat tgacgcagcc ggagcgcgtc gaggcctcca
      961 ctcggcggct gctgcaggcc gccgccgagt tgattgttga aaagggttgg gacgcaatca
     1021 cagctgccga aatcgggcgg cgggcgggtt acagccgggc gatggtgcac gctcgatacg
     1081 gcagcaagga cgccattctg gaggccttcc tgcacgaata tgtgaagcga ctcaaccccg
     1141 atccagatcc caagaccagc ggcatgcagc aggtgcttgc gcacttcgac cgcatagaag
     1201 agatctacac cgaggaccac gaactgctat tggccatgtt cgtcgcaacc ttcgaggcgg
     1261 tgaagacgac atcaccgctg cgtcagcgcg tcagacatca gctgacgagc ggtgccgcga
     1321 aggtcgagaa gggactgcgg aagggcatcc gcgacggttc ggtccgttcc ggcatcgaca
     1381 tcgcccgcac cgtcagtgac atcagcacat cggtcttcgg catcgccttc tggtggaccg
     1441 ccctgcccga ccaatacgac ctcgggcgcg agttgacgaa cgtcagaacg cgcatagtcg
     1501 agaattacgg cggcccagca ctcgagtagc agtcgcacct cagtcgtagc agcaaaaccc
     1561 cagttcacgc tgctgcacgt cccgcccctt gcttgtatcg tgggtttgtg ctcataatat
     1621 gaatgcgtcg tgcgtcacac tgaatcacag ctgtgccacg cctcaccaga caaggacaag
     1681 ccgttcgaag gggccgtacc catgtcacat accgcagtcg tcatcaacac cgcggtcgcg
     1741 atcatcgcgg tcgttgtgat gatcgtgcgc ttcaagttca acccggtcgt gtcgttggtg
     1801 gtgggatccg cctacctcgg cctggcagtc ggacttggcg tggaaaagac gatcgacgcg
     1861 atcatgacgg gattcggcga catcatggcc gaggtcggtc tgctgatcgc gttcggcgtg
     1921 ttgatgggcg cgatactgca gcagacgggg gccattcagc ggctggtgca aacgctcctg
     1981 aacctgttcg ggccaaagcg catgccctat gcgatgtcgc tgacgattgc gacgctgctc
     2041 cagtcgatct ttctcgatgt gctgcttgtc atttcggcgc cgctgggccg cagcctggcc
     2101 aagaagatcg gcaagaacgg aaccgcgcgg atggcggcgg ccatggccat cgggctcgaa
     2161 tgcggaatcg tcctgaccgt cccgggtgtt ggcgcgcttg cgctggcagg cctgctcgga
     2221 gtgccgctgg gcaagtacct gttgttcggt gtgctcctcg taatcccgac cgtggcgatc
     2281 tccgtggcga tcatgtcgtt cctgttcaat cacggctttt ggaacccgga gcgcgacgag
     2341 caggagttcc tcggcgagga tccagagccg ctcgacgatg agcgcgagac cgtaagcgcc
     2401 acaaccacac ccgacgatga cggctcaacc ggtgggggtg tcgcgaccaa gacggcaacg
     2461 gtcacccgtg cccaaacccc gctgatcgtg ctcttcgcac cgatgctggt gtctctcgtc
     2521 ctcattgcga caggggcgat cctcgacgcc ggcgacattc acaaccccat cgtcaagttc
     2581 atctcctccc ccgtcatcgc actgttgatc ggcctgatcg gcacgagctt cgtcggtcgc
     2641 tacacgcttg gggcagagcc gattcagcac gcgatcgcca ccggcttcaa ggagagcggc
     2701 cagatcctca tcctgactgg ggtgggcggc tcgctggccg ccaccatcaa agcggccggc
     2761 ctcggcgaca tcctgggcga gtacttcacc gcgagcaccg ccgccccact gcttgtcgta
     2821 tgggcgatcg ccgcagcact gcacgtcgcc gtcggctccg tgaccatctc cgcgatcacg
     2881 tcggcaggca tcctcgctcc cgtcgcgccg gtcatcgggc ttgaccccgt gctgatcgca
     2941 ctggcggccg gtgccggttc actgttcgcg gtccatgtca cgagcaatac gttctggctg
     3001 ctgcagtcgc tgttgggcca gtcgacgcgc ggcgccttga agaccgtcac cgtcggcgtc
     3061 tccgtcgcat ccgtcgtcgc gatcctgctc atcctgccga tgagcgttct gttctgatat
     3121 gcagcaagga acaacgttga acaccacaga cattcgacca cttgccggcg tgcgggtgct
     3181 ggagctcggg aactacatcg ccgcacccac cgctggccgg atgctcgccg atttcggcgc
     3241 cgaagtcatc aaagttgaac ggccggtcac cggcgacgag ctgcgcaact ggcggctcta
     3301 ctccggggac acgtcgatgc tgtaccggac catcaaccgc aacaagaagt ccatcgtgct
     3361 cgacctcaag accgagcgcg gccgcgagat cgtgctcgaa ctggtccggc actgcgatgc
     3421 gctgctggag aacttccgtc ccggcacgct cgagaaatgg gggctcggcc ccgaagaact
     3481 cgaccgcgtc aaccccaagc tggtgatcac ccgcatctcc gcgttcggcc agaccggacc
     3541 gctatcccaa agacccggat tcgccgcggt ggccgaggcg ttcggcggcc tgcgtgagct
     3601 ggtcggcgat cccgaccggc cgcccgtgcg gaccggtgtc tcgatcggcg actcgatcgc
     3661 cggcatctac gccgcgttcg gcactgtgat ggcgctgttt cagcgggaaa gcgtcaaagt
     3721 gcctctgccc cagcgtgtta tcgacgtcgc cctcaatgaa tccgtgctgt cggtgatgga
     3781 gtcgctggtg ccggactacc tggcctacgg cgtcacgcgg gaacgggtcg gcggccgcat
     3841 ggaaggcatt gcgccgagca acgcctaccc ctgcgcccac ggcaccagca tcatcgtcgc
     3901 cggcaacggg gacgcgatct tccagcgcta catgcaaacc atcggccgcc ccgacctcgc
     3961 cgacgatccg gacctgcaga ccaacgccgg ccgctggcgc cgccgcgacg aactcgacgc
     4021 ggcgatcggc gcttggacca tccaacgcag tcgtgacgag gcgctcaagc tgcttgacga
     4081 ggccggcgtg ccatcgggcc cgatctacac cgccgccgac atctgcgccg acgagcagta
     4141 cgaagctcga aacatggtcc agcacttcga cgttgacacc ggcgagccgc agccgaaggc
     4201 cgtcggcttt cccggcatcg tgccggtgat cggcggggcg tcgctgccga ttcgcagcat
     4261 cggacccgac ctcggcgagc acacccacga ggtgctgtcg acggtgctgg gcctcagcga
     4321 cgacgagata ggagcgctga catgacatcc cgcccctaca caccccgcgc ggagcttcgt
     4381 gatgtgacac tgcgcgacgg gctccaactc accgcgaaga tgctgcccac cgagcgcaag
     4441 gtgcagatcg tccgcgagtt gctcgcgctg ggcgttccgg cgctggagat cgggtcgatg
     4501 gcacgcccgg atctcgtgcc cccgcttgcc gatacgctcg atgtcatcgc ggggctggac
     4561 gccgatgagc tcgagcactg ctggatctgg gtggccacgc cacggcacgt cgagaaggcg
     4621 gcggccgcgg gcgcgcgcaa cttccagtac tgcttttccg cgtcggactc gcacaacaag
     4681 gccaacatcg ggcgcagcac cgaggacagt gtcgcggcga tgcccgatgc cgtccagatc
     4741 gcgcgggagg ccggcggctc catccaactg tgcatcgcaa cggcgttcac atgtccgttc
     4801 gagggcgtca ccgatcccga gcgggtgatc ggcatcgccg ccgacccacg cacagacggg
     4861 gccgacgaca tcgtcgtgtg cgacaccatc gggcaggcca tcccaacgca ggtgaccgac
     4921 ctgatcgaag gcctgacagc gcgaagccct cagcgacgca tcgtttttca cggacacgac
     4981 acctggggcc tcggcgtcgc gaacaccctc gccgcgatcg acgccggagc cacgatgatc
     5041 gacggatcgc tgggcggcct cggcggctgc ccgttcgcac ccggcgccag cggcaacacc
     5101 tccaccgagg acctgctgtt cgcgacccgg ccggactggt tcacgcccgc caccctcgca
     5161 tcgatggtgg acatcaccga caagcttctc gccgagctcg gcgaaccgaa ccgatccagg
     5221 accgctcagg gcgcgcggtc caaggccaag gccttcgact gggtcatcga tcgcggcgcg
     5281 tgaccgcagg tacattcacg gcatggcaac cgacgactcc gcggcctcgt ccaagcagca
     5341 gccactgctg gtgctcggca agatcacgga gatcctcgac gccttctctc tgacacatcc
     5401 cgatatgtcg ctcggtgaga ttcagcaggc aaccgggctt ccgacctcca ccgtgcaacg
     5461 gctggtaagc aatatggtcg cccagggcct gctggaccga gcgggtgacc gcattcgggt
     5521 cggggtgcgg atgtcctact gggccgcgac agccctcaag gacctcgacg tgttggccgt
     5581 cgtcaacccg gtgctcaagg agatccgcga caagaccggc gaaaccgcct gcttcttcaa
     5641 ggtcgaacag aacttccggg tctgcgtcgc ggtcgccgag actcaccatg ccctgcgccg
     5701 agacatgtac gtgggcaagg tcatcccgct gcacgtgggg tccgcatcgc gaatcctgct
     5761 ggcgtggaat ccagacctgg cgtccgcaat cctcgccggg ccgctcgaac cgatgaccga
     5821 cggaacggtc accatcccgg acgagttgcg caggctgatc gctcaggcga aggccgacgg
     5881 ttacgcgatc accgcaggcg aacgcgagga ctcggcatcc ggactgtccg cgccggtgtt
     5941 cgacgccgcg ggcgacatcc tgggcgcgat cacgatcagc ggcccgaccg tgcgcatgcc
     6001 gcaggcgcag tgcgaggcat ggatcgacct gctagtcggg tacgccgagc aggtcacccg
     6061 gacaatcggc ggacgccttc cgcactgagc ccgccgagga accgctccga caccgcttgc
     6121 actcggcgcg agaagaggtg gcctgcgtgg cttccgtcgt gccagaacag ttcgccaccc
     6181 caccgttcgt gtagcgcaat ggcgggctcc cgcaacgcca ttcggtcgta ccacgcgccg
     6241 acaatcaggc ggcgttcggg tggcggcgtc ggctcggtgg cgagcacatc caccggcgac
     6301 gccaaccgcg ccacggtcgc cgatcccagc acgtcgctga catcccgcac cgagggtccc
     6361 caccgtccaa ggtgctgcgc gatcatcgcg ttgagcccga agatcggggt atacaccgcc
     6421 acggcgtcca ccggttcgag atgcgacacc agggctgcga ctggactgcc catggacaca
     6481 ccggatatcg caatagcggt ggtctgcggc cgcagccagc gcacaaccgc tcggacctcc
     6541 gacaccgcgc gcatcattcc ggcgacattg gccagcggat ccatgttcgg ataggggggc
     6601 catgccttgc gacggatgcc gtggcccggc tgcaccggca gcgcgatgtt cacaccgagc
     6661 tcgttctgga tgcggcgggc tcgtgagaac acgaggtcca acggctgtcc ctggcccgcg
     6721 ccgtgcaccc ataccagcca cggccgcggc tggtcgtcac gtcggcagag gtgcacggct
     6781 gcggtcgcgg gcccggcgag gcccccggcg gccagcgagc ccggcagcag ggggtcgtgc
     6841 tcgaatgtca gccgctcata taccatcgtt ccaaaccgtt gtcggcgaat ctccttcacg
     6901 tgcaacggtt ccgggtcagc atgagcctga gatcccagtt ctgataattc gtcggccgca
     6961 gcctggcacg cctgcaccga gcgctccagc ttcggcggcg gtgccgtcag cgtcatgccc
     7021 gtcagcgctg cctcgtccat gagcacctca ccgaggagac gaaggtcggt cgacgacagc
     7081 ggacgccatc cgtcagtccg cagcgctgca tacgcccgcg gcagcacgcc agcgacatcc
     7141 cggccgatcc gccacgctcg ttcggacgtt ttcatcggct acgcgagctt gaagccggtg
     7201 tatccggcgg ccgcgatctc gtcgcaccgg cgtcggtatt cggggattcc cgccgtgtat
     7261 cccatgtaca tccgtttctt gccgggaaca ttgcccccgt tgtaccagga gttgcacgtc
     7321 gggtggacga gcaccgtcgg cgcaaccagc gaggtcgtgt gttcgatcca ctcgcgttga
     7381 gcgtcgggca gcgcttcgat cgtttggtag tcgtggctgc atagataagc gatgcagtca
     7441 ccgatccact ccacatgctg ctccagggcg gcgacgaaat tcgttgccgc cgaggg
//
LOCUS       DASRJA010000233        20680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1757255,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000233 DASRJA010000000
VERSION     DASRJA010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20680
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757255"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..868
                     /gene="ppk2"
                     /locus_tag="VFC01_10575"
     CDS             14..868
                     /gene="ppk2"
                     /locus_tag="VFC01_10575"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HZN80117.1"
                     /translation="MTLDSHGYRVHDDDDDDPILLDGNGIPVDTWRENFPYDERMSRD
                     EYEEQKRLLQIELLKLQKWSQANGHRHVIVFEGRDAAGKGGTIKRFMEHLNPRGARVV
                     ALEKPTERERTQWYFQRYVHHLPAHGEIVLFDRSWYNRAGVERVMGFCSPKQHAEFIR
                     QVPLFEQMLVNDGISLTKLWFSVSASEQRTRFTIRQVDPVRQWKLSPTDLASLDKWDS
                     YTAANEDMFAWTDTEIAPWMVVKSNDKKRARVNAMRYVLNKFDYDNKDHEVVGRPDPL
                     IVGRALND"
     gene            900..1751
                     /locus_tag="VFC01_10580"
     CDS             900..1751
                     /locus_tag="VFC01_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80118.1"
                     /translation="MRFVATVFLWLVTTVVLAAAIPAMWTQKNIVDANGYAAFAQSAA
                     KDPKLQQAMASELTTQIVSLASNSGYDLNPDLVRGVTTAYTQNAGFPGQFAQANRIVH
                     TWMFTDEIRRQDGTDDRWLIDIAPMLSDSSLRATLGNLNLDVPATLTVPITVSNTGGM
                     QPGQLRQLSTWGPWVSVGATVLTGVFALLTLAAARARGKALAALGVSALLVGAAGWAG
                     LEVARRYVNHALDRTTGDIRQVADAMVNHAEGGLHQWLNLTLAAGGVLVVFGVLVTML
                     GGLRRRD"
     gene            complement(1748..2209)
                     /locus_tag="VFC01_10585"
     CDS             complement(1748..2209)
                     /locus_tag="VFC01_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006359549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HZN80119.1"
                     /translation="MTFDTPNGSKGARQPKAGRVMRWLNTFASNRIRKSGKFMGGMNA
                     LVLTTVGRKSGVERSTPVGWFPGDDGSWLIVASAAGAPNNPAWYYNIAAHPDKVRIEL
                     AGRTVDVVAEQLHGAAREDAWRQITSAASRFAQYQEKTDRELPIIRLVPRS"
     gene            complement(2219..2650)
                     /locus_tag="VFC01_10590"
     CDS             complement(2219..2650)
                     /locus_tag="VFC01_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZN80120.1"
                     /translation="MGLFTDKEKEYLQQQALGRLATSGKTGMPHVVPTGFHLDPETGN
                     IKIGGHSYRGQDRLYIRHLKVNPNAAFAVDDLVTDPTWTPRGVTIKGPVRIHESGGEA
                     LGPGFGPRWIELIPQWVSSWGIDADPFAAPTAPRKLADADR"
     gene            complement(2704..3222)
                     /locus_tag="VFC01_10595"
     CDS             complement(2704..3222)
                     /locus_tag="VFC01_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005082710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN80121.1"
                     /translation="MDAARGVFANLGYERATIRGIAAAAGVDKSSVIQYFGTKQNLFR
                     EAVHWTIPVADAIGDTPEQTAENLARGMLAAWAADPNSPMAVLLRASMTSEDAAEILR
                     NHITSSATPTLATAIDAPDARLRAALLGAMLMGVASQRYLLQMPDVADVDIDKLVRLI
                     TPALQALISPAE"
     gene            3408..4228
                     /locus_tag="VFC01_10600"
                     /pseudo
     CDS             3408..4228
                     /locus_tag="VFC01_10600"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
     gene            4247..4651
                     /locus_tag="VFC01_10605"
     CDS             4247..4651
                     /locus_tag="VFC01_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN80122.1"
                     /translation="MADRPEVPMNPQIIEEFRANGGKVGGPFEGATLLLLHTRGAKSG
                     KERVNPVMYFRIDNKILVVGSYAGADVDPAWVHNLRANPRAHIEIGTDSYDVEVRQLS
                     SDERDAAYPRIVETAPGFGGYQEKTDRVIPVF"
     gene            complement(4671..5276)
                     /locus_tag="VFC01_10610"
     CDS             complement(4671..5276)
                     /locus_tag="VFC01_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80123.1"
                     /translation="MTPALTAKGQATRQRMLDAAAAHLAAHGTIEVARIAGAAGVAPS
                     VLYRYFDGRDGLVAAVVEDFYDSYEERVFARRDVPGNTWPVREALRLEREIAFFYDHP
                     LGRAVACGLLREPAAAQVDAERTRAQGQAAARNIRAGQRAKELSPAIDAGLAGAAIIG
                     AVRVMLSEALARTPPARQRDVVKAALKIGAAVLGTGDLALP"
     gene            complement(5273..6178)
                     /locus_tag="VFC01_10615"
     CDS             complement(5273..6178)
                     /locus_tag="VFC01_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZN80124.1"
                     /translation="MSTSSQFETLLYSTDGPVATITLNRPEHLNTIVPPMPDEIEKAV
                     GLAERDPAIKVIVLRGAGRAFSGGYDFGGGFHHWGDAMMTDGKWDPGKDFAMVSAHET
                     GPTQKFMAIWRASKPVIAQVHGWCVGGASDYALCADIVIASDDAVIGTPYSRMWGAYL
                     TGMWLYRLSLAKVKWHSLTGEPLTGKEAATVELINESVPFEDLEARVSEVAGKLCQIP
                     LSQLRAQKLIVNQAYENMGLASTQTLGGILDGLMRNTPDALEFIETAATQGVRTVIER
                     RDGPWGDYSQAPPERRPDPSHVIVP"
     gene            complement(6232..7098)
                     /locus_tag="VFC01_10620"
     CDS             complement(6232..7098)
                     /locus_tag="VFC01_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN80125.1"
                     /translation="MTADVDIDKIMNPPARVQTMRRAVDTLQDGLGPFFDIYRPYVDG
                     LQSLPKDGRFLLVGNHTQFGIEALLIPHYVRRVIGTRVRPLADRGMGQMRGAAGDLFA
                     AYGAVVGAPETARELMRHNESILVFPGGGREIAKFKGEENTLRWQGRAGFARVSVENT
                     YPIVPVALVGADQVYRSLITRDSRWGRLSLSLTEKVFGRRDMALPLLHGVGPTLIPRP
                     QRMYLRFAAPIDTTKPARVTEQEWVDAVKRNTQESLEQAIADLLEIRASDPYRELNPL
                     AWRAAIQLPAEG"
     gene            complement(7095..7883)
                     /locus_tag="VFC01_10625"
     CDS             complement(7095..7883)
                     /locus_tag="VFC01_10625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN80126.1"
                     /translation="MGETTAVDPAIAEMPRGGQNASWLDRLLQTDALEYTDRYDVPDA
                     VKQKVISALDWMGTRTGMHEKNARVACALVADIPNPRILELGAGHGKLSAKILELHPT
                     ATVTISDLDPTSVGNIAAGELGDHRRVRTQVVDATAIDADDDSYDLVVFAQAFHHLPP
                     AIAYRAIAEATRVGKRFLVIDLKRRSLLGLILAPLVMAPLAVVPSLKPVMHDGFISGL
                     RAYSSSAFVALGEAADPQMRIEFLPSASRFGAPTVVVFSRASQS"
     gene            7967..8536
                     /locus_tag="VFC01_10630"
     CDS             7967..8536
                     /locus_tag="VFC01_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN80127.1"
                     /translation="MRSTTELRGEILSAARAEFAQYGLAGARIDRIARNAQASKERLY
                     AHFGDKETLFREVFAADSAEFFRSVSMRPDAVPEFVGDIFDLALRRPEHLRMISWAHL
                     EGFTLDEPQADGQPIYAQGLATIEAAQTSGHVDPAWQPADLMVLLFSIGLGWANLPHP
                     DVATDDPTVIAQRRAAAVEAATRIVAART"
     gene            8586..9671
                     /locus_tag="VFC01_10635"
     CDS             8586..9671
                     /locus_tag="VFC01_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020105717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN80128.1"
                     /translation="MLRVIQWATGSVGRHAIAAVHEHPNLELVGALVYSDAKTGRDVG
                     DLCGIGPIGITATKDPDEILALDADCVLYMPQGEMNPMGALDDICRLLSSGKNVVSTA
                     VTGLIYPRSLGDKVVDKLESACTEGQTSFHATGIEPGWAAEVLPLTMSGLFQRIDSLL
                     VQELMDYTTYDSADMLFDIMGFGKAPDEPVPMADAALAGVTFRAPLMLLADGLGATID
                     DFVYHREVALAAEAFDIKAGRVEAGTVAAQRFGYTAIVDGRPALTVEHITRLGDEQAP
                     DWPTGRGWKVTVEGRPSMVLDSKIATHGEDETDQGCLGTAMHAIHAIAPVCAAAPGIR
                     TFLDLPTIIGRGVLSASGAGTRISTQP"
     gene            complement(9610..11625)
                     /locus_tag="VFC01_10640"
     CDS             complement(9610..11625)
                     /locus_tag="VFC01_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZN80129.1"
                     /translation="MDDDDPYLWLEDITGDDALDWVRRHNEPTLAELGGDRFEQMRAE
                     ALEVLDTDARIPYVRRRGEYLYNFWRDAQNPRGLWRRTTLDSYRTEEPEWDVIVDVDA
                     LAKADDENWVWAGVEVLEPDYSLALIELSRGGADASIVREFDMEKRGFVADGFQLPEA
                     KSGVSWEDRDTVLVGTDFGPGSLTESGYPRIVKRWRRGQPLADAQALFSGDPSDVSVG
                     GGYDHTPGFERLLITRSLDFFNRERYELRGNELIRVDVPTDAGISLHREWLLIRPRTE
                     WAVGTTIYPAGSLLAAKYDEFLAGTAELSVVFEPDAHTSLQNYTWMRDHLVLVTLVDV
                     ASRVQIISPGSWETAAIGGIPPNTNTVLVDVDEYGDEMFLDSSGFDVPSRLLWGRAGG
                     EVTEIKRAPSFFDAADLEVNQHFATSDDGTAIPYFVVSHRHVDAPGPTLLTGYGGFEN
                     SSLPTYGGVMGRLWLSRGGTYVLANIRGGGEYGPTWHTQAMRESRHLVAEDFAAVARD
                     LVERGTATVEQLGAYGASNGGLLMGIMLTQYPELFGALVCSVPLLDMRRFHLLLAGAS
                     WVAEYGDPDEPDDWEFISKYSPYQNVSTGRRYPPVLITTSTRDDRVHPGHARKMTAAL
                     EAASQPVQYYENIEGGHAGAADNAQTAFRAALIYEFLHRTLTAHRGQ"
     gene            complement(11648..12274)
                     /locus_tag="VFC01_10645"
     CDS             complement(11648..12274)
                     /locus_tag="VFC01_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006550772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN80130.1"
                     /translation="MQLEHRLSVPAPIDVVWPALLDPERVAPCVPGATLTGVEGETFT
                     GTVKVKVGPITLLYKGTGTFTETDEQGRRAVLKADAKDTRGNGTVKATVTLTLTDEGD
                     HTTGTVATDLSITGKPAQFGRGMIADVGGKIIEQFAACLSEKLAVAESESAPASAGQP
                     AVEASETEPLDLVQYARSSALKRVGPVVAVAMLVLILGAVVRRLRSRD"
     gene            complement(12277..13131)
                     /locus_tag="VFC01_10650"
     CDS             complement(12277..13131)
                     /locus_tag="VFC01_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZN80131.1"
                     /translation="MIPSNFDYVAPKTVDDAVAALAQAGEDAKVIAGGQSLMPVLRLR
                     LAAPTTLVDLNKVDELRGVREDGDALVIGAMTTYYDLLRNPLVSQHALLLSEATHTVA
                     DPQIRHRGTLGGNLAHADPAGDLAAPVLALDATLTVAGPSGRRSIPVAEFFVDYFTTA
                     LQPDEILTEIRIPKHTNWAARYEKLHRVAQAWSIVAVAATVDVDGGTIRQARIALTNM
                     VSVPVRARGVEEALVGQAASEALIRAAAEHAAEGLDPMTDGNADADYRSHLAKVLTRR
                     AVATAVGA"
     gene            complement(13128..15503)
                     /locus_tag="VFC01_10655"
     CDS             complement(13128..15503)
                     /locus_tag="VFC01_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZN80132.1"
                     /translation="MTATAEPEIGRARVRKEDEHLITGRTRWTDNIVLPGMQHLAILR
                     SPVAHARITGIDTSAARSMPGVVAVFTGADFAAEQGSLPNAWQITEDMKAPPAPSLAV
                     DTVNFAGEAVAVVVARNAYEAQDALEGIDVDYDDLPVVLDLAAAAADGAEVVHPDLGT
                     NVSAVWTFDSAEAGTGGDVEDAIRDGEVVLQRTFRQQRLIPAFMEPRSVVVDPTSPQL
                     TVWSATQIPHILRLMLAMTLEIPEHKVRVIAPDVGGGFGGKLQVTPEEVIALLVARRL
                     GKPVKYTESRSESMLAAHHGRDQIQKLTLAARRDGTVTGLKVELLADMGAYLRLVTSG
                     IPILGAFMFNGIYKFPSYHFTCTNVFTNKVPTDAYRGAGRPEATFAVERMMDELAAEL
                     GRDPLELRAQNWITHDEFPFDTVAGLTYDSGNYEAATEQARELFDYDGLRREQAERRE
                     RQDPIQLGIGISTFTEMCGLAPSRVLGSLSYGAGGWEHAAIRMLPTGKVEVVTGSSPH
                     GQGHETAWSQIVADQLGVPFEDVEILHGDTQSSPRGLDTYGSRSLAVGAIAVVKAAEK
                     VIAKAKPFAAHMMECAEDDLEFTEGRFRVTGTEKAVTLAEVALAVFAAHDLPDGLEPS
                     LDAEATYDPENFSFPHGTHLCATEVDTDTGQVRIRSYVCVDDIGHVVNPLIVDGQVHG
                     GLAQGIAQALYEEAVHDETGTLINASFSEYLVPSAADLPSFTTGRTETPATTNPLGVK
                     GVGEAGTIASTPAVVNAVVDAVRHLGVKDIEMPCSPMRVWHAITEAQGGAR"
     gene            complement(15500..16030)
                     /locus_tag="VFC01_10660"
     CDS             complement(15500..16030)
                     /locus_tag="VFC01_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005570244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN80133.1"
                     /translation="MRISVTVDGTTYTDEVEPRLLLVHYLRDRLGKVGTVVGCDTSNC
                     GACTVALNGYSVKSCSVLAVQADGGDVMTVEGLARDGGKLHPVQEAFRDNHALQCGFC
                     TPGMIMQTIDLLAEDPDPDEAAVREGLEGNLCRCTGYQNIVRAVQDAAQRLRATATDE
                     SPKDVAAKADTAGGVR"
     gene            complement(16113..17204)
                     /locus_tag="VFC01_10665"
     CDS             complement(16113..17204)
                     /locus_tag="VFC01_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN80134.1"
                     /translation="MPELVGVAGFTRALAVAGLPVASDAVETYTRALRQIDLGDPGQV
                     YWAGRATLCRGPDDIPRYDLVFENWFGGTVPSRAPHATQRQRRSSIATLDAAGAAETG
                     GDAPRLRVAAADTEILRHRDIGELTAAERAHLAELLAALRPRSPTRPALRLRPSRRGR
                     IDPRRTVRHMLAAGGEPVRPRHQRKATRPRRIVLLLDVSGSMSPYADALLRFAHVVAR
                     TNPATEVFSLGTRMTRLSRPLRIRDPELALAAAGRAVPDWAGGTRLGETMRAFLDRWG
                     RRGLARGAVVVVFSDGWERGDPRLLADQMAAMRRLAHAVLWVNPHAGADGYQPVQSGI
                     AAALPFIDRLLAGHSLATLQALLDEIRDA"
     gene            complement(17206..18099)
                     /locus_tag="VFC01_10670"
     CDS             complement(17206..18099)
                     /locus_tag="VFC01_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN80135.1"
                     /translation="MSDSAPRVESPSDLAQALEATGYLADEGVATAAYLALRMGRPLF
                     CEGEPGTGKTSLAAALAEAMRLPLIRLQCHEGIDAAQALYDWDFPRQLLHLRTLEAAS
                     AGHLEAEAAERSLYTERFLLARPLLRALTEAPCVLLVDEIDRADDEFEAFLLEVLDDN
                     AVTIPELGEIRAATPPLVVLTSNRTRDVHDALKRRCLYHWLEHPDLAREVAILRRRIP
                     GIAEALADQVARAVHILRGMELVKPPGVAESLDWARALRELDRDVLDAQAAAATLGAV
                     VKYHEDLERVARAGLDRLLAG"
     gene            18204..18482
                     /locus_tag="VFC01_10675"
     CDS             18204..18482
                     /locus_tag="VFC01_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin"
                     /protein_id="HZN80136.1"
                     /translation="MIALPRAWLLASAMLVGIAVGQIAGIAATLLVDATIRPDVVIAL
                     VVGIPSAIGMLLILFSGRRWVTVLGAFILAIAPGWLGVLVTIQVVHGA"
     gene            18475..18951
                     /locus_tag="VFC01_10680"
     CDS             18475..18951
                     /locus_tag="VFC01_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80137.1"
                     /translation="MHNDTLPGEPTQPYLPAFTGEFPAQPDPVQVLDTPSGPLPALAQ
                     PEVVPGTYQYLKRWTFVLVVAGVWILSAASGWGLYYWWFHSLDKTPPVFVVLLFVIVC
                     TVGSLLAAMVSDKPLVSALSVGLMSAPLAALGAAAVLHGLYFCDRVSRCLVGVIPY"
     gene            18962..20368
                     /gene="lpdA"
                     /locus_tag="VFC01_10685"
     CDS             18962..20368
                     /gene="lpdA"
                     /locus_tag="VFC01_10685"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZN80138.1"
                     /translation="MTHYDVVVLGAGPGGYVAAIRAAQLGLNTAVVEPKYWGGVCLNV
                     GCIPSKALLRNAELAHIFTKEAKQFGISGEATFDYGVAFDRSRKVADGRVAGVHFLMK
                     KNKITEIHGYGTFTDPNTLSVKLNEAGGAENPVTVTFDNIIIATGSSTRLVPGTSLSE
                     NVVTYEELILSRELPASIVIAGAGAIGMEFGYVLKNYGVDVTIVEFLPRALPQEDAEV
                     SKEIEKQFKKLGVKVLTGTKVESIKDNGGEVIVSVSRDGKNDEIKAEKVLQAIGFAPN
                     IEGYGLETAGVKITDRKAIGIDDYMRTNVPHIYAIGDVTGLLQLAHVAEAQGVVAAET
                     IGGAETLPLGDYRMLPRATFCQPQVASFGLTEEQARDEGYDVKVAKFPFTANAKAHGV
                     GDPSGFVKLIADGRYGELIGGHLIGHDVSELLPELTLAQKWDLTATELARNVHTHPTM
                     SEALQECFHGLVGHMINF"
     gene            20365..20634
                     /locus_tag="VFC01_10690"
     CDS             20365..20634
                     /locus_tag="VFC01_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80139.1"
                     /translation="MRAIWVAGIGGLVVGHILWLAGISVAIATGSVSTWVLVVAAVSF
                     VVGAVAAYFGWRSYKKKSEIWAAFLWALPVAPVLLSLTVLGVTYL"
BASE COUNT         3384 a         6783 c         6870 g         3643 t
ORIGIN      
        1 atattttttg ccggtgactc tggattcaca cggctaccgc gtgcacgacg acgacgatga
       61 cgacccgatc ctgctcgacg gcaacggcat tcccgtcgac acctggcggg agaacttccc
      121 gtatgacgag cgcatgtcac gcgacgagta cgaggagcag aagcgactgc tgcaaatcga
      181 attgctgaag ctgcagaagt ggagccaggc caacggccac cggcacgtca tcgtgttcga
      241 gggtcgcgac gcggcgggca agggcggcac gatcaagcgg ttcatggagc acctcaaccc
      301 gcgcggcgcc cgcgtcgtcg cgctggagaa gccaaccgag cgcgaacgca ctcagtggta
      361 cttccagcgc tacgtccacc acttgcccgc ccacggcgag atcgtgctgt tcgaccggtc
      421 ctggtacaac cgagcaggcg tcgagcgggt gatgggcttc tgctcgccca agcagcacgc
      481 ggagttcatc cgtcaggtcc cgctgttcga gcagatgctg gtcaacgacg ggatcagtct
      541 gaccaagctg tggttctcgg tgtcggcgtc tgagcagcgc acccggttca ccatccgcca
      601 ggtcgacccg gtgcggcagt ggaaactgtc gccgacggac ttggcctcac tggacaaatg
      661 ggactcatac actgcggcca atgaagacat gttcgcgtgg accgacaccg agatcgcgcc
      721 gtggatggtg gttaagagca acgacaagaa gcgtgcgcgt gtcaacgcga tgcgctacgt
      781 gttgaataag ttcgactacg acaacaagga ccatgaggtg gtcggcagac cggaccccct
      841 catcgtggga cgtgccctga acgattgagg cctgcgcgtc ctgcccgaag ggaggacgcg
      901 tgcggttcgt ggcgaccgtg ttcctgtggc tcgtcacgac ggtcgtgctt gcggcggcga
      961 tcccggcgat gtggacgcag aagaacatcg tcgacgcaaa cggctatgcg gcgttcgccc
     1021 aatcggccgc caaggatccc aaactgcagc aggcgatggc ctcggaactc accacgcaga
     1081 tcgtgtcgct ggcgtcgaat tcgggctatg acctcaaccc ggatctggtg cgcggtgtga
     1141 cgacggcgta cacccagaac gccggtttcc ctgggcagtt cgcgcaggcg aaccggatcg
     1201 tgcacacctg gatgttcacc gacgagatcc gtcgtcagga cggcaccgat gatcgatggc
     1261 tcatcgacat cgccccgatg ctgtcggact cgtcgttgcg ggcgacgttg ggcaatctca
     1321 accttgacgt gccagcgacg ttgactgtgc ccatcaccgt ttccaatacg ggagggatgc
     1381 agccggggca gttgcggcag ctgtcgacgt ggggtccgtg ggtcagcgtc ggtgcgacgg
     1441 tgctgacggg agtgttcgcg ctgctgacgt tggcggcggc cagagcccgc gggaaagcac
     1501 ttgccgcgct gggtgtttca gcgctgctgg tgggtgccgc aggctgggcc ggcctggaag
     1561 tggcgcgtcg ttacgtcaac cacgccctgg atcgcaccac aggcgatatc cgtcaggtcg
     1621 ctgacgcgat ggtcaaccac gccgagggcg gtttgcatca gtggctcaac ctgacgctgg
     1681 ccgcgggcgg tgtgctggtc gtgttcggcg tgctggtgac gatgctgggt gggctgcggc
     1741 gccgagatta ggagcggggg accagccgga tgatcggcaa ctcgcggtcc gtcttttcct
     1801 gatactgcgc gaaacgagat gcggcggagg tgatttgccg ccaggcgtcc tcgcgtgctg
     1861 caccgtgaag ctgttctgca acaacgtcta ccgtgcggcc ggcaagctcg atccggacct
     1921 tgtccgggtg tgcggcgatg ttgtagtacc acgccggatt gttcggcgcc ccggccgctg
     1981 atgcgacgat caaccagctg ccgtcgtcgc cgggaaacca tccgaccggt gtactgcgtt
     2041 caacgccact cttgcgcccg accgtcgtga gcaccagcgc gttcatgcca cccatgaact
     2101 tgccgctctt gcgaattcga ttggatgcaa acgtgttgag ccaccgcatc acgcgtccgg
     2161 ccttcggctg gcgggcacct ttggagccgt tcggcgtgtc gaaagtcatg tgatctcctc
     2221 agcggtcggc gtcagcgagc ttacgcggcg ctgtcggcgc agcgaacggg tcggcgtcga
     2281 tgccccacga cgaaacccat tgcgggatta gttcgatcca gcgcggaccg aagccgggtc
     2341 caagagcttc gccgccggat tcgtgaattc gcaccgggcc cttgatggtt acgccccgcg
     2401 gcgtccacgt cgggtcggta acgaggtcgt cgaccgcgaa cgcagcgttt ggattcacct
     2461 tgaggtggcg gatgtacagc cggtcctgac cgcggtagga atgcccgccg atcttgatat
     2521 ttccggtttc tgggtcgagg tgaaatccgg tcggcactac gtgtggcatg ccggtcttcc
     2581 cggacgtggc cagccggccc agtgcctgct gttgcaggta ttccttttcc ttatcggtaa
     2641 acagtcccat ctgagttctc cttcggtccg agatgtccga gaaaactcta caactgtcta
     2701 tttttattcc gcgggggaaa tcagcgcttg cagggcaggg gtgatgaggc gcacgagctt
     2761 gtcgatgtcg acgtcggcga cgtccggcat ctgaaggagg tagcgctggc tggcgacacc
     2821 catcaacatc gcgccgagca atgccgcgcg cagccgcgca tcgggtgcgt cgatcgcggt
     2881 cgcgagggtg ggtgtggcgc tggaggtgat gtggttgcgc aggatctcgg cggcgtcctc
     2941 gctggtcatg cttgccctta gcaggacggc catagggctg ttggggtcgg cggcccacgc
     3001 ggccagcatg ccgcgcgcca ggttctcggc ggtctgctca ggcgtatcgc cgatggcgtc
     3061 ggcgaccgga atggtccagt gcactgcttc gcggaacagg ttctgcttgg tgccgaagta
     3121 ctggatgacc gatgacttgt cgacgccggc cgcggccgca atgccgcgga tcgtcgcgcg
     3181 ctcgtagccg aggttggcga acacgcctcg ggccgcgtcc aggatccggc gacgggtgcg
     3241 ctgtcctttg cgctcaccgc cctcgctggt cgcgggcgac tcaggcaccg gctccgaggt
     3301 cacggcagta atcgtacaaa gcgccgggga ccgggaatag ccaaaagtag acaaccgttg
     3361 agaaatccga cggactatga gaggagtcat catcagcgcc ggtggcggtg gtcggggcaa
     3421 cagggcagca gggatcggcg accgtcgatg cgctgctcga tcgaggtgtt ccggtccgcg
     3481 ccctgacccg caacaccgac tcggccgcgg cccgagcgtt ggccgatcgc ggtgtgcaag
     3541 tggtggcggc ggacttggac acacctgagt cggttcagcg cgcgacggtg ccgcggccgc
     3601 gttcgcgatg acaacgttcg gcgaccctgg gggcatcgac ggtgaagtcg cgcaaggcaa
     3661 ggtgatcggt gaggcggcgc aggcggccgg tctgccgttt ttggtctata gctcggtcgg
     3721 tggtgccgaa agacattcgg gtataccgca tttcgaaagc aagggcagga acgagcagat
     3781 cttgatcggt ggcgtcccac tcaatatcgt gcggccgacc ttcttcatgg agaacctgac
     3841 ctacatgatc ggccgcgacg gcgacgagtt ggcggtgcgg ctgccgatgc ccggagatgt
     3901 gccgctccaa atgatttcgg tgcgtgacat cggcaaggtc gccgctgcgc tgcttgcgct
     3961 gcgagaccct gccgcggcgc cgattgagat agcaggcgat gaggtgaccg gcgagcagat
     4021 cgtcgaacga ctcggacaac gactaggtgc acccgcgaag ttcgtcgaac taccactcga
     4081 cgcgctcggc gaggacgatg accagaagac gatgttccag tggttcacca agctgccggc
     4141 atatcgcgcc gacttcgagc gcacccgcga actcgtcccc gatgtcgaag acctgcccac
     4201 ctggctgcag cgccaatcgc tcggctgatt cggagaggac accaacatgg ctgatcgccc
     4261 agaagtaccg atgaaccccc agatcatcga ggaattccgc gccaacggcg gcaaggtcgg
     4321 cgggcccttc gagggcgcca ccctgctcct gctgcacacc aggggtgcga agtccggcaa
     4381 ggaacgagtc aatccggtga tgtacttccg catcgacaac aagatcttgg ttgtcggttc
     4441 gtacgcaggt gccgacgtgg acccggcgtg ggtgcacaac ctgcgggcca acccgcgcgc
     4501 ccatatcgaa atcggcacgg actcttacga tgtcgaggta cggcagctca gtagcgacga
     4561 gcgcgacgct gcctatccgc gcatcgtaga aacagcaccg ggcttcggtg ggtaccagga
     4621 gaagaccgac cgcgtcattc ctgtcttttg aactgtcggc gctcggcggg tcacggcaga
     4681 gctagatcac cggtgcccaa caccgcggcg ccgattttga gcgcggcctt gacgacatcg
     4741 cgttgccggg cgggtggcgt gcgggcgagc gcctcggaga gcatgacgcg cacggcgccg
     4801 atgatcgcgg cgccggccaa ccccgcgtcg atggcaggcg agagttcttt tgcgcgctgc
     4861 ccggcgcgga tgttgcgcgc ggccgcctgt ccttgggcgc gggtccgttc ggcgtcgacc
     4921 tgtgcggcgg cgggttcgcg cagcaggccg caggcgacgg cgcggccgag gggatggtcg
     4981 tagaagaagg cgatttcgcg ttcgagcctg agcgcttcgc gcaccggcca ggtgttacca
     5041 ggaacgtcgc ggcgcgcgaa gacccgttcc tcgtagctgt cgtagaagtc ctcgaccacc
     5101 gcggccacca gcccgtcgcg gccgtcgaag tagcggtaca gcacgctcgg ggcgacgccg
     5161 gcagccccgg cgatccgggc tacttcgatg gtcccgtgcg cggcaagatg tgcggcggcg
     5221 gcatccagca tccgctgtcg ggtcgcctgg ccctttgcgg tgagcgcagg cgtcacggca
     5281 cgatgacgtg cgacgggtcg ggtcgccgct cgggcggggc ctgactgtag tcaccccatg
     5341 ggccgtcgcg gcgctcgatc acggttcgaa caccttgcgt cgcagcggtt tcgatgaact
     5401 ccagcgcgtc gggggtgttg cgcatcagcc cgtcgaggat gccaccgagc gtctgagtgg
     5461 acgcgagccc catgttctcg taggcctggt tgacgatcag cttctgggcc cgcagttgcg
     5521 acagcgggat ttggcacagc ttgcccgcca cttccgagac tcgtgcttcg agatcctcga
     5581 agggcaccga ctcgttaatg agttcgactg tggcggcctc ctttccggtc agcggttcac
     5641 cggtcagcga gtgccatttc accttggcga gggacagacg gtagagccac atgccggtga
     5701 ggtaggcgcc ccacattcgg ctgtacggag tgccgatgac ggcgtcgtcg ctggcgatca
     5761 cgatgtcggc gcacagtgcg tagtcgctgg cgccgccgac gcaccagccg tgaacctggg
     5821 cgatcacggg tttggatgcg cgccagatgg ccatgaactt ctgcgttgga cccgtctcgt
     5881 gcgcggagac catcgcgaaa tcctttccgg ggtcccactt tccgtctgtc atcatcgcat
     5941 cgccccagtg gtgaaagccg ccgccgaagt cgtagccgcc ggagaatgcc ctgccggcgc
     6001 cgcgcagcac gatcaccttg atggcggggt cgcgttcggc caagccgacg gccttctcga
     6061 tttcgtctgg cattggcgga acgatggtgt tcaggtgttc gggacggttc agcgtgatgg
     6121 tggcgacggg accgtcggtc gaatacagca gcgtctcgaa ctgggacgaa gtcgacataa
     6181 cagaagtgaa atcgtttctc ttctgaaagt caaggttggg ttttggcgcc gtcatccttc
     6241 ggccggtagc tggatggcgg cgcgccatgc cagcgggttc agttcgcggt acgggtcgct
     6301 ggcacggatt tccaagaggt cggcgatggc ctgctccagt gactcctggg tgttccgttt
     6361 gaccgcgtcc acccactcct gttcggtgac tcgcgcaggt ttggtggtgt cgatcggcgc
     6421 agcgaatctc agatacattc gctgcggtcg gggaatcagg gtggggccga ccccatggag
     6481 cagtggcagc gccatgtcgc ggcgaccgaa cactttctcg gtgagactta ggctcagccg
     6541 tccccatcgg ctgtcgcggg tgatcaggct ccggtaaacc tggtcggcgc cgacgagcgc
     6601 cacaggcacg atcggatagg tgttctccac cgatacgcgt gcgaatccgg cacgaccctg
     6661 ccagcgcagg gtgttctcct cgcccttgaa cttggcgatc tccctgccac cgccgggaaa
     6721 caccagaatg ctctcgttgt gtcgcatcag ctcgcgcgcg gtctccggcg ccccgacgac
     6781 agcgccgtag gccgcgaaca gatcgcctgc cgcgccgcgc atttggccca taccacggtc
     6841 ggcaagcggc cgcacccgcg tgccgatcac gcgccgcaca tagtgcggaa tcaagagggc
     6901 ttcgatgccg aattgggtgt ggttgcccac caacaggaat cgcccgtctt tgggcaggct
     6961 ctgcaggcca tcgacgtatg gccggtagat gtcgaaaaac ggtccgagtc cgtcttgaag
     7021 agtgtcgacc gcgcggcgca tcgtctggac acgcgcgggt ggattcatga ttttgtcgat
     7081 gtccacgtcg gcggtcatga ctgcgacgcc cgggagaaca ccacaacggt cggggcgccg
     7141 aatcgcgaag ccgacggcag gaattcgatc cgcatctgcg ggtcggctgc ctcgcccagt
     7201 gcgacgaacg ccgaggagct gtatgcccgc agtccgctga taaacccgtc gtgcataacg
     7261 ggtttcaagg acggcacaac cgccaacggc gccatcacca gtggtgccag gatcagcccg
     7321 agtaaggacc gccgtttgag gtcgatcacc aggaagcgct taccgaccct cgtggcctcg
     7381 gcgatggcgc ggtaggcgat ggccggcggc aagtgatgaa acgcttgcgc gaaaacgacc
     7441 aagtcgtagc tgtcgtcgtc ggcatcgatc gcggtggcgt cgaccacctg ggtgcgcacc
     7501 cgccgatggt cgcctaattc gccggcagcg atgttgccca ctgaggtcgg gtccagatcg
     7561 ctgatcgtca ccgtcgcggt cggatgcaac tccaggatct tggccgacag cttgccgtgc
     7621 cctgcaccca gctcgaggat gcgtgggttg gggatgtcgg cgaccagcgc gcacgcgacg
     7681 cgagcgttct tctcgtgcat gccggtgcgg gtgcccatcc aatcgagcgc ggagatgacc
     7741 ttctgcttca ccgcatcggg gacgtcatag cggtcggtgt actccagcgc atcggtctgc
     7801 aatagccgat ccagccacga agcattctga ccgccccgcg gcatctccgc aatcgccgga
     7861 tcgaccgccg tcgtctcacc catcacaagc tccttgtact tagtcgttct aacctgacct
     7921 cgaccacgct accaaacgaa ctagttcgtt tggctatgat cttcctgtga gaagcacgac
     7981 cgagctgcgc ggcgagatct tgtcggccgc ccgggctgaa ttcgctcagt acggcctggc
     8041 cggcgcccgg atcgaccgca tcgcgcgcaa cgcacaggcc agcaaggaac gcctctacgc
     8101 ccacttcggc gacaaggaga cgctgttccg cgaggtgttc gcggccgaca gcgccgagtt
     8161 cttccgctcg gtctcgatgc gacctgatgc agtccccgag ttcgtcgggg acatcttcga
     8221 tctggcgcta cgccgacccg agcacctgcg catgatcagt tgggcacatc tggaaggttt
     8281 caccctcgac gaaccccagg ccgacggtca acccatctac gcccagggtc tcgccactat
     8341 cgaagccgcg caaaccagcg ggcacgtcga ccccgcatgg cagcccgccg accttatggt
     8401 cttgctcttc tccatcggcc ttgggtgggc caacttgcct caccccgatg tggctaccga
     8461 cgaccccacg gtcatcgcgc agcgtcgcgc cgccgctgtc gaggcggcta ccaggatcgt
     8521 cgccgcgcgc acctgagagc cgaaaccgac ggctcaatcg tctcccttac agtaacctcc
     8581 acagcatgct gcgcgtcatt cagtgggcca ccggatccgt cggccgccat gccatcgccg
     8641 ccgtccacga acatcccaat ctggagctcg tcggtgccct cgtctacagc gacgccaaga
     8701 ccggccgcga cgtcggcgac ctctgcggca tcggccccat cggcatcacg gcgaccaagg
     8761 accccgacga gatcctcgcc ctcgacgccg actgcgtcct ctacatgccc cagggcgaaa
     8821 tgaacccgat gggcgcgctc gacgacatct gccggttgct gtcctccggc aagaacgtag
     8881 tgtcgaccgc cgtcaccggg ctcatctacc cgcgcagcct tggtgacaaa gtggtcgaca
     8941 agctggagtc ggcctgcacc gagggccaga cgtccttcca cgcgaccggc atcgaacccg
     9001 gctgggccgc agaggttttg ccactgacaa tgtcaggcct ctttcagcgc atcgactccc
     9061 tgctggtcca agaactgatg gactacacca cctacgacag tgccgacatg ctgttcgaca
     9121 tcatgggctt cggcaaggcc cccgacgaac ccgtgcccat ggccgacgcc gcactcgccg
     9181 gggtcacctt ccgcgcaccg ctgatgctcc tcgctgacgg cctcggcgcc accattgacg
     9241 attttgtcta ccaccgcgag gtcgcgcttg ccgcagaagc attcgacatc aaggccggcc
     9301 gggttgaggc ggggactgtc gccgcgcagc gctttggcta caccgccatc gtcgatggcc
     9361 ggcccgcact gaccgtcgag cacatcaccc ggctcggcga cgaacaggcc cccgattggc
     9421 ccaccggccg cggctggaag gtcactgtcg aagggcgacc gtcaatggtg ctcgactcga
     9481 agatcgccac ccacggtgag gacgaaaccg atcagggctg cctgggcact gccatgcacg
     9541 ccatccacgc catcgccccg gtctgcgcgg cggcgccggg aatccgtacc ttccttgacc
     9601 ttccgacgat cattggccgc ggtgtgctgt cagcgtccgg tgcaggaact cgtatatcaa
     9661 cgcagccctg aacgccgtct gcgcattgtc ggccgcacct gcgtggccgc cctcgatgtt
     9721 ctcgtagtac tgcaccggtt ggctcgccgc ctccagcgct gcggtcatct tgcgggcgtg
     9781 gcctggatgc accctgtcgt cacgcgtgga cgtggtgatc aacaccggcg gatagcgccg
     9841 gcccgtcgaa acgttttgat acggcgagta tttggagatg aactcccaat cgtcgggttc
     9901 gtctgggtcg ccatactcgg cgacccacga tgcgccggcc agcagcagat ggaaccgccg
     9961 catgtccagc agcggcacac tgcacaccag tgcaccgaac agctctgggt actgcgtcag
    10021 catgatgccc atcagcaggc caccgttgct cgcaccgtag gcgcccaact gttcgacggt
    10081 cgcagttccg cgctccacca gatctcttgc cacagcggca aagtcttcgg ccaccagatg
    10141 tctgctctcg cgcatcgcct gcgtatgcca cgtcggcccg tattcgccac caccccggat
    10201 gttggccaac acatacgttc cgccgcgcga cagccataac cggcccatca ctccgccgta
    10261 tgtcggcagg ctcgagttct cgaaaccgcc gtagccggta agcagcgtcg gcccgggtgc
    10321 gtccacatgt cgatggctga cgacgaaata ggggattgcg gtgccgtcgt ccgacgtcgc
    10381 gaagtgctgg ttcacctcga gatccgcggc gtcgaagaac gacggtgccc gcttgatctc
    10441 tgtcacttca ccgcctgctc gcccccacag cagccgggag gggacgtcga aaccgctgga
    10501 gtccaggaac atctcgtcgc cgtactcgtc cacgtcgacc aagacggtgt tcgtgttagg
    10561 ggggataccg ccgatcgctg cggtctccca cgaacccggc gagatgatct gcacccggct
    10621 cgcgacgtcg acgagggtga cgagaacgag gtgatctcgc atccacgtgt agttctgcag
    10681 gctggtatgc gcgtcgggtt cgaaaacgac actcaattcg gctgtgcccg ccaggaattc
    10741 gtcgtacttt gccgccagca gcgatcctgc cgggtagatc gtcgtgccca cggcccattc
    10801 ggtgcggggc cggatcagca accactcgcg atgtagtgag atgccggcgt cggtcggcac
    10861 gtcgacccgg atcaactcgt tgccgcgtag ctcgtagcgt tcccggttga agaagtcgag
    10921 ggaccgggtg atcaggagcc gctcgaaccc gggcgtgtgg tcgtacccgc cgcccacgct
    10981 cacgtcagac gggtcgcccg agaacagcgc ctgcgcgtcc gcaagcggtt gtccacgccg
    11041 ccaccgcttc acgatgcgcg ggtagccgga ttcggtcagc gaccctgggc cgaagtcggt
    11101 gccgaccagc acggtatcgc ggtcctccca cgagacaccg gacttggcct ccggcagctg
    11161 gaagccgtct gcgacgaatc cgcgtttctc catgtcgaat tcgcgcacga tgctggcgtc
    11221 ggcgccgccg cgtgacagtt cgatcagcgc aaggctgtaa tcgggttcga ggacttcgac
    11281 gccggcccac acccagttct cgtcgtcggc cttcgccaac gcgtcgacgt cgacgatgac
    11341 gtcccactcc ggctcctcgg tacggtagct gtccagcgtc gtgcgtcgcc acagtccgcg
    11401 cgggttctgt gcgtcgcgcc agaagttgta caggtactcg ccgcggcgcc tcacatacgg
    11461 gatgcgggcg tcggtatcga gcacctcgag cgcctcggcg cgcatctgct cgaagcggtc
    11521 accgcctaac tcggcaagcg tgggctcgtt gtgcctgcgc acccagtcca gcgcgtcgtc
    11581 gccggtgatg tcctcaagcc acagatacgg gtcgtcgtcg tccacgcacc cattcttgcc
    11641 ggacgggtca gtcgcgcgac cgcaggcgtc gcaccaccgc gcccaaaatg agcaccaaca
    11701 tggccaccgc caccaccggt ccgacccgct tcagggccga actccgcgcg tactggacca
    11761 gatccagggg ctcggtctcc gaggcctcca ccgctggctg cccggccgac gccggcgcgg
    11821 attccgattc tgcgacggcg agtttctccg aaaggcacgc ggcgaactgc tcgatgatct
    11881 ttccgccgac gtccgcgatc atgccccggc cgaactgggc aggcttgcca gtgatcgaca
    11941 ggtccgtggc cacggtgccg gtcgtgtggt cgccttcgtc ggtcagcgtg agggtgacgg
    12001 tcgccttcac ggtgccattc ccgcgggtgt ccttcgcgtc ggccttgagc acggcgcgcc
    12061 tgccctgctc gtcggtctcg gtgaacgtcc cagttccctt gtacagcaac gtgatcgggc
    12121 cgaccttgac cttgacggtg ccggtgaacg tctcaccttc gaccccggtc agtgtcgcgc
    12181 cgggcacaca cggcgccacc cgctcgggat cgagcagcgc cggccacacc acgtcgatgg
    12241 gagccggtac cgacaggcgg tgttccagct gcatggtcag gcacccacag ccgtcgccac
    12301 cgcccgccgt gtcagcactt tcgccagatg gctgcgatag tcggcgtcgg catttccgtc
    12361 ggtcatgggg tcgaggcctt cggcggcatg ctcggcagcc gcgcgaatca acgcctcgct
    12421 cgcggcctga ccgaccagcg cctcctcgac gccgcgggcg cgcaccggca ccgacaccat
    12481 gttggtcagc gcgatgcgcg cctgccggat cgtcccgcca tcgacgtcga cggtcgccgc
    12541 gaccgccacg atcgaccagg cttgcgccac ccggtgcagt ttctcgtacc gggccgccca
    12601 attggtgtgc ttcggaatcc ttatctcagt gaggatttcg tctggttgca gcgcggtggt
    12661 gaagtagtcc acgaagaact ctgcgaccgg aatgctgcgc cggcccgacg gacccgcgac
    12721 cgtcagcgtg gcatcgagcg ccagcaccgg tgcggccaaa tcaccggccg gatcggcgtg
    12781 cgccaggttt ccgcccagcg tgccgcggtg ccggatctgc ggatccgcca cggtgtgggt
    12841 ggcttccgaa agcagcagcg cgtgctggct gacgagcgga ttgcgcagca ggtcgtagta
    12901 ggtggtcatc gcgccgatca ccagggcgtc gccgtcctcg cgcaccccgc gaagctcgtc
    12961 gactttgttc aggtcgacga gggtggtcgg ggcggccagc cgcagccgca gcaccggcat
    13021 caggctttgc ccgcccgcga tgaccttcgc gtcctcgccg gcttgcgcaa gcgccgccac
    13081 cgcgtcgtcg acggtcttcg gggcgacata gtcgaaattc gacgggatca tcgcgcacct
    13141 ccctgagcct cagtgatcgc atgccagact cgcattgggc tgcaaggcat ttcaatgtcc
    13201 ttgacgccga gatgccgcac cgcatcgacg accgcgttga ccaccgccgg agtggaggcg
    13261 atggtgcccg cctcaccgac acccttgacc ccgagggggt tggtggtggc cggggtttcg
    13321 gtccgtccgg tggtgaacga cggcagatcc gccgcggacg gcaccaggta ctcggagaac
    13381 gacgcgttga tgagcgtgcc ggtctcgtcg tgtacggcct cttcgtagag ggcctgtgcg
    13441 atgccctggg cgagtccgcc gtgcacctgt ccgtccacga tcagcgggtt gaccacatga
    13501 ccgatgtcgt cgacgcagac atacgagcgg atccgcacct gcccggtgtc ggtgtcgacc
    13561 tccgtcgcgc acagatgcgt gccatgcggg aacgagaagt tctccgggtc gtaggtggcc
    13621 tcggcatcga ggctcggttc cagaccgtcg ggcaggtcgt gcgcggcgaa cacggcgagc
    13681 gcgacctcgg cgagcgtgac ggccttttcg gtacccgtca cgcggaaccg tccctcggtg
    13741 aattcgaggt cgtcctcggc gcattccatc atgtgcgccg cgaagggttt ggccttggcg
    13801 atcacctttt ccgcggcctt gaccaccgcg atcgcgccga ccgccagtga tcgcgacccg
    13861 taggtgtcca agccgcgcgg cgagctctgg gtgtcgccgt gcaggatctc gacatcctcg
    13921 aatggcactc cgagttggtc ggcgacgatc tggctccacg cggtctcgtg gccctgaccg
    13981 tgcggcgacg agccggtgac cacctcgacc ttgcccgtcg gcagcatgcg gatcgccgca
    14041 tgctcccagc cgcccgcccc gtacgacagc gaaccgagca ctcgcgacgg ggccagaccg
    14101 cacatctccg tgaacgtgga gatcccgatg cccagctgta tcgggtcctg gcgctcgcgg
    14161 cgctcggcct gttcgcgccg caatccgtca tagtcgaaca attcgcgggc ctgctcggtg
    14221 gcggcctcgt agttgcccga gtcgtaggtc agccccgcga cggtgtcgaa cgggaactcg
    14281 tcgtgggtga tccagttctg cgcgcgcagc tcgagcggat cacgcccgag ctcggcggcc
    14341 agctcgtcca tcatccgttc gacggcgaac gtcgcctccg ggcggcctgc gccgcgatag
    14401 gcgtctgtcg gcaccttgtt ggtgaacacg ttggtgcatg tgaagtgata cgacgggaac
    14461 ttgtagatgc cgttgaacat gaacgcgccc aggatcggga tgcccgaggt gaccagccgc
    14521 aggtaggcgc ccatgtcggc gagcagttcc accttgagcc cggtgacggt gccgtcgcgg
    14581 cgggcagcga gcgtcagctt ctggatctgg tcgcgcccgt ggtgcgccgc gagcatgctc
    14641 tcgctgcggg actcggtgta cttgaccggc ttgccgagcc tgcgcgcgac gagcagggcg
    14701 atcacttcct ccggcgtcac ttgcagcttg ccgccgaagc cgccgccgac atccggggcg
    14761 ataacccgta ccttgtgctc cgggatttcc agcgtcatcg cgagcatgag ccgcaggatg
    14821 tgcgggatct gcgtggccga ccagacggtg agctgcgggc tggtcgggtc gaccaccacc
    14881 gagcggggtt ccatgaacgc cgggatcagc cgctgctgcc ggaacgtgcg ctgcaatacc
    14941 acctcgccgt cgcggatggc gtcctcgaca tcgccacctg tgcctgcctc cgcggagtcg
    15001 aatgtccaca ccgcgctgac gttcgtgccg agatcggggt gcaccacctc cgcaccgtcg
    15061 gcggcggccg cggcgagatc gagcaccacc ggcaggtcgt cgtagtcgac gtcgattcct
    15121 tcgagcgcgt cctgcgcttc gtacgcgttg cgggccacca cgacggccac ggcttcgccc
    15181 gcgaagttca cggtgtccac cgcgagtgac ggagcgggcg gtgccttcat gtcctcggtg
    15241 atctgccagg cgttgggcag gctgccttgc tcggcggcga agtcggcgcc ggtgaagacc
    15301 gcgacgacac cgggcatgct gcgcgcggcg ctggtgtcga taccggtgat gcgggcatgg
    15361 gcgacgggac tgcgcaagat ggccaggtgc tgcatccctg gcaacacgat gttgtcggtc
    15421 caccgggtgc gaccggtgat cagatgctcg tcttccttgc gaacccttgc cctgccgatt
    15481 tcgggttcgg ccgtggcggt catcgcacac ctccagccgt atcagctttg gctgcaacat
    15541 ctttcgggga ttcgtcggtt gcggtggcgc gcagccgctg tgccgcatcc tgcaccgccc
    15601 tgacgatgtt ctggtatccg gtgcagcggc acaggttgcc ttccaggccc tcgcggaccg
    15661 cggcttcgtc cgggtcgggg tcctcggcga gcaggtcgat ggtctgcatg atcatgcccg
    15721 gcgtgcagaa gccgcactgc aacgcgtggt tgtcgcggaa cgcctcctgg acggggtgca
    15781 gcttgccgcc gtcgcgggca aggccttcga cggtcatcac gtcgccgccg tcggcttgca
    15841 ccgcgagcac cgaacaggac ttgacgctgt agccgttgag cgcaacggtg cacgcgccgc
    15901 agttgctggt atcgcagccg accacggttc cgaccttgcc gagccggtca cgcaggtagt
    15961 gcacgaggag aagccgcggc tccacctcat cggtatacgt cgttccatcg acggtgacag
    16021 agatgcgcat aggcctactc tgctctggct gacagccgag aacgcccgaa acgacgaaaa
    16081 cgattctggg acaacctgac tgccgggctg aatcaggcgt cgcggatctc gtcgagcaaa
    16141 gcctgcagcg tcgccaggct gtgcccggcc agcaaccggt cgatgaacgg caacgccgcc
    16201 gcgatcccgg actgcaccgg ctgatatccg tcggcgccgg catgcgggtt cacccacagc
    16261 accgcatggg caagccgccg catcgcagcc atctggtcgg cgagcagacg cgggtcaccg
    16321 cgctcccagc cgtccgagaa gaccaccacg acggcgccgc gggccaggcc gcgtcggccc
    16381 caacggtcca ggaaagcgcg catcgtctcg cccagccggg tgccgccggc ccagtccggc
    16441 accgctcggc ccgcggccgc caacgccaat tcgggatcgc ggatgcgcag cggccgcgac
    16501 agccgggtca tgcgggtgcc cagggagaac acctcggtgg ctggattggt gcgtgcaacg
    16561 acgtgcgcga agcgcaggag agcatccgcg taggggctca tcgagccgga tacgtcgagc
    16621 agcaacacaa ttcgccgcgg ccgggtggcc ttgcgttgat gtcgaggccg caccggttcg
    16681 ccgcccgcgg caagcatgtg gcgcactgtg cgccgggggt cgatacggcc gcggcgggac
    16741 ggccgcagcc gcaaggcggg ccgggtcggg gagcgtggcc gtagcgcagc gaggagctcg
    16801 gcgaggtgcg cccgttcggc cgcggtcagt tcgccgatat cgcggtggcg caatatctcg
    16861 gtgtcggccg cagccacccg cagtcgtggc gcgtcaccgc cggtctcggc ggcgccggcc
    16921 gcatcgagcg tggcgatgct ggagcggcgt tggcgttgag tcgcgtgtgg ggcgcggctg
    16981 ggcaccgttc ccccgaacca gttctcgaac accaggtcgt agcgcggaat gtcgtccggt
    17041 ccgcgacaca gggtcgcgcg gccggcccag tacacctgcc ctgggtcgcc aagatctatc
    17101 tgacgcagcg cccgtgtgta ggtttcgacg gcgtccgacg ccacgggcag ccccgcaacc
    17161 gcgagggcac gcgtgaatcc tgcgacaccg acgagttccg gcatgtcacc ccgcgagcag
    17221 tcggtccagc ccggccctgg ccacccgctc caggtcctcg tggtacttca cgaccgcgcc
    17281 tagcgtcgcc gcggcggcct gcgcgtcgag cacgtcgcgg tcgagttcgc gcagggcgcg
    17341 cgcccagtcc agcgattcgg cgacacccgg aggcttgacc agctccatgc cgcgcaggat
    17401 gtggacggcg cgcgccacct ggtcggccag cgcctcggca atgccgggta tgcggcggcg
    17461 aaggatcgcg acctcacgcg ccaggtcggg atgctcgagc cagtggtaca ggcagcgccg
    17521 tttgagggcg tcgtgcacgt cgcgggtgcg gttcgacgtc aggacgacga gcggcggcgt
    17581 tgcggcccgg atctcgccga gttcggggat ggtgacggcg ttgtcgtcga gcacctcgag
    17641 caggaaggcc tcgaattcgt cgtcggcgcg gtcgatttcg tcgacgagca gcacgcacgg
    17701 cgcctcggtg agtgcccgca gcagcggtcg cgcaagcagg aaccgctccg tgtagagcga
    17761 gcgttccgcg gcttcggcct cgaggtgccc ggcgctggcc gcctcgagtg tcctcaggtg
    17821 cagcagctgc cgcggaaagt cccagtcgta gagcgcctgc gccgcgtcga tgccctcgtg
    17881 gcactgcagc cggatcagcg gcaggcgcat cgcctcggcc aacgcggcgg caagggacgt
    17941 cttgccggtg ccaggttcgc cttcgcagaa cagcggtcga cccatgcgca acgccaggta
    18001 cgccgccgtc gcgacgcctt cgtcggcgag gtagccggtg gcctcgagtg cctgcgccag
    18061 atccgatggc gactcgactc tgggcgcgga atcgctcacc tttgcgagcc taggtcaggg
    18121 ctcgatcaca gctcggcatc ttcacgcgtg acctcgcgtg gcgcaaagcc gggccccgcg
    18181 aatgcggcgt tatcgtcgga ctcgtgatag cgctaccgcg tgcgtggctg ttggccagcg
    18241 cgatgctcgt gggcatcgcg gtcggacaga tcgcgggtat cgcagcgacg ctgctggtgg
    18301 acgccacgat ccggcccgac gtcgtcatcg cgctcgtcgt cggtattccc agcgcgatcg
    18361 gcatgctgct gatcctgttc tcgggccgac gctgggtcac cgtgcttggc gcgttcatcc
    18421 tggccatcgc ccccggatgg ttgggagtgc tcgtcacgat ccaggtggtc cacggtgcat
    18481 aacgacacgt tgcccggcga gccgacgcag ccctacctgc cggcattcac cggagagttt
    18541 cccgcgcagc ccgatccggt acaggtctta gatacgccgt cggggccact tcccgcgctg
    18601 gcccagcccg aggtggtgcc gggcacctac caatacctca agcgttggac cttcgtcctc
    18661 gtcgtggcgg gggtatggat cctctcggcc gcgtccgggt ggggcctgta ctactggtgg
    18721 ttccactcgc tcgacaagac accgccggtg ttcgtggtgc tgctctttgt gatcgtctgc
    18781 acggtcggca gtctgctggc cgcgatggtg agcgacaagc cgctggtttc ggcgctgtcc
    18841 gtcggcctga tgtcggcgcc gctcgctgct ctcggcgccg cggccgtgct gcacggcctc
    18901 tacttctgcg atcgggtaag ccgctgtttg gtcggtgtca tcccctacta gggttgggcg
    18961 ggtgacccac tatgacgtcg tcgttcttgg cgcaggccct ggcggatatg tcgcggccat
    19021 tcgcgccgcc cagctcggac tgaacaccgc agtagtcgag cccaagtatt ggggcggggt
    19081 atgcctcaat gtcggctgta tcccgtccaa ggcgctgctg cgcaatgccg aactcgccca
    19141 catcttcacc aaggaagcca agcagttcgg catcagcggc gaggcgacgt ttgactacgg
    19201 ggtggcgttc gaccgcagcc gcaaggtcgc cgatggccgg gtcgccggtg tgcacttctt
    19261 gatgaagaag aacaagatca ccgagatcca cgggtacggc accttcaccg atccgaacac
    19321 gttgtcggtc aaattgaacg aggcaggcgg cgctgaaaac ccggtgacgg ttacgttcga
    19381 caacatcatc atcgccaccg gcagcagcac ccggttggtg ccgggcacgt cgctgtcgga
    19441 gaacgtggtc acctacgaag agctgatcct gtcgcgtgag ctgcctgcgt cgatcgtcat
    19501 cgccggtgcg ggcgcgatcg gcatggagtt cggctacgtg ctgaagaact acggcgtcga
    19561 cgtgaccatc gtcgagttcc tgccgcgcgc gctgcctcaa gaggatgccg aggtctccaa
    19621 ggagatcgag aagcagttca agaaactcgg cgtgaaggtg ctgaccggaa ccaaggtcga
    19681 gtcgatcaag gacaacggcg gtgaggtcat cgtctccgtc agcagggacg gcaagaacga
    19741 cgagatcaag gccgagaagg tgctgcaggc gattggcttc gcaccgaaca tcgagggcta
    19801 cggcctggag acggccgggg tgaagatcac cgaccgcaag gccatcggca tcgacgacta
    19861 catgcgtacc aacgtgccgc acatctacgc catcggtgat gtgaccgggt tgctgcagct
    19921 ggcccatgtg gccgaggcgc agggtgtggt ggccgccgaa accatcggtg gggcagagac
    19981 tttgccgctc ggggactatc ggatgctgcc gcgtgccacg ttctgtcagc cccaggtggc
    20041 cagcttcgga ctgaccgagg agcaggctcg cgacgagggt tacgacgtga aggtcgcgaa
    20101 gttcccgttc acggccaacg ccaaggcgca cggtgtggga gacccgagcg gatttgtgaa
    20161 gctgatcgcc gacggccgct acggcgagct gatcggcggg cacctcatcg gacacgatgt
    20221 ctccgagctg ctgccggagc tgaccctggc gcagaagtgg gacctgaccg ccaccgagct
    20281 ggcccgcaac gtgcacaccc acccgacgat gtccgaagcg ctgcaggaat gcttccacgg
    20341 tctcgtcggc cacatgatca acttttgaga gcgatctggg tcgccggtat cggcggattg
    20401 gtggtcggcc acatcctgtg gctggccggt atttccgtgg cgatcgcgac gggatcggtc
    20461 agcacctggg tgctggtcgt cgccgcggtg tcgttcgtcg tcggcgccgt cgccgcgtat
    20521 ttcggctggc gctcgtacaa gaagaagtct gagatctggg cggcgtttct gtgggcgcta
    20581 cccgtcgcgc cggtgttgct gtcgctgacc gtgctagggg tgacctacct atagtcaggc
    20641 catgacggca gcacggcggc gactgctcat ggccaacggt
//
LOCUS       DASRJA010000234         4659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1088691,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000234 DASRJA010000000
VERSION     DASRJA010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088691"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFC01_10695"
     CDS             complement(<1..185)
                     /locus_tag="VFC01_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HZN80140.1"
                     /translation="MSEHQNAQSSATRPEKDVVASRLAYLTVGLPTAGTVGAIAYAVY
                     DGLWVSDVVLFAVMYVLT"
     gene            complement(200..1846)
                     /locus_tag="VFC01_10700"
     CDS             complement(200..1846)
                     /locus_tag="VFC01_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HZN80141.1"
                     /translation="MSEGLAYIFLADGTSESEIRWTFADTAKNSRRFAAHLGDRGIGV
                     GDRVVLAVNPSLEYVAALYGIMELGAIPVPCFPPVRPKELGRFHAITRDCSPAAIVID
                     EMYREQVGALRGFLKSSNLEPVVVYTEHVPAGTADVDVVPPAPDDLALIQYTSGSTGS
                     PKGVCLTHDNLVSNCEALSRSMGHDPQRVGMSWLPPYHDMGLIGTIIMSMYDGSPLVL
                     MSPIHFVQDPRRWLKAITDYRASVTVGPNFALDLCVDVVAHEDSEDLDLSTVKELFCG
                     AEPIHAGTLARFEQTMAPLGFDGKALIPCYGMAEATLFVAGKRSGSSCRTARAPELSG
                     TGGVVVSCGAVDNEHTVRIVDTTTLTSLEDGLVGEIWVSGRSVAAGYHNRPELTREMF
                     HARIQGEDREYLRTGDLGFIQSGELFVTGRIKDLIIVDGRNIYPHDVEAAVVSADPAL
                     RRAVAFSIDGRGSEELCVVAEAGTGEVAAESYSRMVESIICCVTAEFGVRPRVNLCPR
                     RTIPTTTSGKVRRQLARQMFLANGFPSIETDLSAMRGRTA"
     gene            complement(2275..>4659)
                     /locus_tag="VFC01_10705"
     CDS             complement(2275..>4659)
                     /locus_tag="VFC01_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZN80142.1"
                     /translation="PNRGMFGGDGAYGEAKSALDAVVSRWKAESSWAQRVSLAHALIG
                     WTKGTGLMGHNDAIVGAVEEAGVTTYTTDEMATMLLGLCDIESKVAAAREPLQVDLTG
                     GLAEIDLDMSELAAKAREEMKTEITEDDAQDDGTIRALPSPPRGYQAAPPPAWDDLDV
                     DPADLVVIVGGAELGPYGSSRTRFEMEVAGELSAAGVLELAWTTGLVKWEDDPKPGWY
                     DTQSGDLIDEGELVGRYHDAVIEKVGIRELVDDGAIDGDHASPLLVSVFLDKDFTFVV
                     SSEADARGFVQFDPEHTVIAPVPDSSDWQVTRMAGTEIRVPRKTKLPRTVGAQIPTGF
                     DPTVWGISQDMANSIDRVAQWNIVATVDAFLSAGFTPAELMRWVHPSLVASTQGTGMG
                     GMTSMQTMYHGNLLGRNKANDILQEALPNIVAAHVVQSYVGSYGAMIHPVAACATAAV
                     SVEEGVDKIRLGKAELVVAGGFDDLTMEAIIGFGDMAATADTEMMRAKGISDSRFSRA
                     NDRRRLGFVEGQGGGTILLARGDLAVRMGLPVLAVVAYAQSFGDGVHTSIPAPGLGAL
                     GAGRGGRDSQLARSLAKLGVGPDDIAVVSKHDTSTLANDPNETELHERLADSLGRSAG
                     APLFVVSQKSLTGHSKGGAAAFQMMGLCQILRDGVIPPNRSLDCVDDELASSGHFVWP
                     RETLWLGDKFPLKAGLITSLGFGHVSALIALVHPQAFIATLSPDKRGDYLTCAAARVL
                     AGQRRLTSAIAGGPALYEKPADRRFSQEAPEKRQEVAMLLDPASRLGEDGLYTP"
BASE COUNT          847 a         1575 c         1415 g          822 t
ORIGIN      
        1 gtgagcacgt acatgacagc aaacaacacc acatcggaga cccagaggcc gtcgtagacg
       61 gcgtacgcta tggcaccgac ggttcccgcc gttggtagcc cgaccgtcaa atacgccagg
      121 cgcgaggcaa caacatcctt ctcaggccgg gtcgcgctgc tctgcgcatt ctgatgttcg
      181 gacattgtga gatctgtagt catgctgttc gccctcgcat tgccgataga tcagtttcaa
      241 tcgacggaaa cccattggcc aggaacatct gcctggccag ctgtcgccgg acctttccgc
      301 tggtcgtcgt tggtattgtt cgtctgggac agagattgac ccgaggtcgt acgccgaatt
      361 ccgcagtgac gcaacaaata attgactcca ccatcctcga gtacgactcc gcggcgactt
      421 ccccggtccc ggcctcggcg accacgcaca actcctcgga gccgcggcca tcgatggaga
      481 aggcaacggc tctccgcaag gcgggatccg cagacacgac ggcggcctcg acgtcgtgcg
      541 gataaatgtt tcgcccatcg acgatgatca ggtccttgat tcgacccgtg acgaatagtt
      601 cacccgactg aatgaaaccg aggtcacccg tgcgcaagta ctcgcgatcc tcgccttgga
      661 tcctcgcgtg gaacatctcg cgggtcagct cgggccggtt gtgataaccg gcggcaacgc
      721 ttcgcccaga cacccagatt tcgccgacga ggccgtcttc caggctcgtg agggtcgtgg
      781 tgtcgacgat gcgcaccgtg tgttcgttgt cgaccgcgcc acagctgacc acgaccccac
      841 ccgttccgga cagctccggc gcgcgggccg tgcggcaact ggaaccgctc cgcttcccag
      901 cgacgaaaag cgtcgcctcc gccatcccat aacaagggat cagcgctttt ccgtcgaaac
      961 cgagcggcgc catggtctgc tcgaaccgcg cgagggtgcc ggcgtggatc ggctcggcgc
     1021 cacagaacaa ctccttgacc gtggaaaggt ccagatcctc gctgtcctcg tgggccacca
     1081 catccacaca gagatcgagc gcgaagttcg gtcccacggt gaccgacgcc ctgtagtcag
     1141 tgatcgcctt cagccagcgc cgcgggtctt gaacgaagtg gatcggtgac atcaagacca
     1201 gcggcgaacc gtcatacatc gacatgatga tcgttccgat cagccccatg tcgtggtatg
     1261 gcggtaacca cgacattccg acccgctggg gatcgtgacc catgctgcgg ctcaaagcct
     1321 cgcagttgct taccaaattg tcgtgggtga ggcacacccc cttcggagat cccgtcgaac
     1381 ctgacgtgta ctgaatcagc gccaagtcat cgggcgcagg tgggacgaca tctacgtcgg
     1441 cggtccccgc ggggacatgc tcggtgtaaa caaccaccgg ttcgaggttg gaggacttga
     1501 ggaaaccccg cagtgcacca acctgttccc ggtacatctc gtcgatgacg attgcggcag
     1561 gcgagcaatc ccgcgtgatc gcgtggaatc ggcccagttc tttgggtcgc acaggaggga
     1621 agcatggaac ggggatcgcg ccgagctcca ttatcccata aagtgcggca acgtattcga
     1681 ggcttggatt caccgccaat acgacgcgat cgccaacgcc gatccctcga tcgcccaagt
     1741 gggcggcaaa cctgcgggaa ttcttcgcgg tgtcggcgaa cgtccaacgt atctcgctct
     1801 ccgaggtgcc gtcggcgaga aatatgtagg cgagaccctc tgacaacgat ctgtcggata
     1861 agacttcgac aatgctcttc gggtggctgt ccacagtatc taagaccccc ttcctcgaag
     1921 atccagtgct ttccgatcaa cgtgcccaac gccgcagtct gcaacagacc cgtcgactag
     1981 taccccgtcg cgcaacacaa acgcgaattc ggttcgcgca cagcacgaca acatcggccg
     2041 ggtgcggaga accccaacca catcaaccga cgccatcaag cttccttccc tcgatatgtc
     2101 ctaaagcggc tcctgctatg gaagaggccg attggcgtct tgcagataca gcaccgctgc
     2161 ttaggtgttt ggtggtgtga tgccggcttg tgacggtttg cgcgacagga aaccgataag
     2221 gaggtcgcca ccgagctgaa tgcgtcacta gcggaaaaga tgtcggcgcg aacgctacgg
     2281 cgtgtagagg ccgtcttcgc cgagccgcga tgccggatcg agcaacatcg cgacctcctg
     2341 ccgcttctcc ggtgcctcct gactgaaccg gcggtcggcc ggcttctcgt acagcgccgg
     2401 cccgccggcg atcgccgacg tgagcctgcg ctgaccggcc agcaccctag ccgccgcgca
     2461 cgtcaagtag tccccccgtt tatcgggact cagcgttgcg atgaacgcct gcgggtgcac
     2521 gagtgcgatc agtgccgaga cgtgtccgaa cccgagactg gtgatcaggc ccgccttcaa
     2581 cgggaacttg tcccccagcc agagtgtttc gcgcggccac acgaagtgac ccgaactggc
     2641 gagctcgtcg tcgacgcagt ccaggctgcg gttgggcggg atcacgccgt cgcgcaggat
     2701 ctggcacagc cccatcatct ggaatgccgc cgcaccaccc ttcgagtgac cggtcaggct
     2761 cttctgcgac accacgaaca gcggtgcacc ggcggagcgg cccaacgagt cggccagccg
     2821 ttcgtgcagc tccgtctcgt tcggatcgtt ggccagcgtc gaggtgtcgt gcttggacac
     2881 caccgcgatg tcgtcggggc cgacacccaa cttcgcgagt gaacgcgcca gctgcgaatc
     2941 cctaccgcca cggcccgccc cgagggcgcc gagaccggga gctgggatcg acgtgtgcac
     3001 accgtcgccg aaggactgcg cgtaggcgac gactgctagc acgggaagac ccatcctcac
     3061 tgcgaggtcg ccgcgggcga gcaggatggt tcccccgccc tgtccctcga cgaacccgag
     3121 ccggcgacgg tcgttggcgc gcgagaacct cgagtcgctg atgcccttgg cccgcatcat
     3181 ctcggtgtcc gcggtcgcgg ccatgtcacc gaagccgatg atggcctcca tcgtcaggtc
     3241 gtcgaagccg cccgcgacca caagttccgc cttgcccagc cggatcttgt ccactccctc
     3301 ctccaccgac accgcggcgg tggcgcaggc ggcgaccggg tggatcatcg cgccgtagct
     3361 gccgacgtac gactgaacca catgggcggc gacgatgttt ggcagagcct cttgcaggat
     3421 gtcgttggcc ttgttccggc cgagcaggtt gccgtggtac atggtctgca tcgatgtcat
     3481 gccgcccatg ccggtgccct gagtgctggc caccaggctg gggtgcaccc agcgcatcag
     3541 ctcggccggc gtgaaccctg cagacaggaa cgcgtcgacg gtcgcgacga tgttccactg
     3601 cgccacccgg tcgatcgagt tggccatgtc ctggctgatg ccccacaccg tcgggtcgaa
     3661 cccggttggg atctgggcgc cgaccgtgcg tggcagcttc gtcttgcgcg gtacccgaat
     3721 ctcggtgcct gccatgcggg tgacctgcca gtcgctgctg tcgggcaccg gcgcgatgac
     3781 ggtgtgctca gggtcgaatt gcacgaaccc gcgggcgtcg gcctccgagg acaccacgaa
     3841 ggtgaagtcc ttgtccagga ataccgaaac cagcagcggc gacgcgtgat ccccgtcgat
     3901 ggcaccgtcg tcgactaact cgcggatgcc gaccttctcg atgaccgcgt cgtggtaccg
     3961 cccgacgagt tcaccctcgt cgatcaggtc accggactgc gtgtcgtacc agccaggctt
     4021 gggatcgtct tcccacttga ccaggccggt cgtccaggcc agttcgagta caccggccgc
     4081 cgacaactcg cccgcgacct ccatctcgaa gcgggtccgc gacgaaccgt acgggccgag
     4141 ctcggcacca ccgacgatga ccaccagatc ggccgggtcg acgtcgaggt cgtcccacgc
     4201 cggcggcggc gcggcctgat aaccgcgcgg cggcgacggc agagcacgga tggttccgtc
     4261 gtcctgcgcg tcgtcctcgg ttatttcggt cttcatctcc tcgcgagcct tcgcggccag
     4321 ctccgacata tcgaggtcga tctcggcaag cccaccggtc aggtcgacct gcagcggctc
     4381 gcgcgccgcc gcgaccttgg actcgatgtc gcacaggccc agcagcatgg tcgccatctc
     4441 gtcggtcgtg tacgtcgtca cgccggcctc ttcgaccgca ccgacgatcg cgtcgttgtg
     4501 gcccatcagg ccggtgccct tggtccagcc gatcagcgcg tgcgccaacg aaactcgctg
     4561 ggcccacgac gactcggcct tccaccgaga caccaccgcg tcgagcgcgg acttcgcttc
     4621 gccataggcg ccgtcgccgc cgaacatgcc gcggttcgg
//
LOCUS       DASRJA010000235        27304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1901957,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000235 DASRJA010000000
VERSION     DASRJA010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 27304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27304
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901957"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            436..1764
                     /gene="hisD"
                     /locus_tag="VFC01_10710"
     CDS             436..1764
                     /gene="hisD"
                     /locus_tag="VFC01_10710"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZN80143.1"
                     /translation="MADFQMTRIDLRNRSLNAAQLRTALPRGGVDVDAVVPKVRPIVD
                     AVAENGAQAALRYGESFDGVRPATVRVPVGELDAALNALEADVRVALQVAIDRARAVH
                     ADQRRTDTTTTLAPGATVTERWVPVERVGLYVPGGNAVYPSSVVMNVVPAQTAGVDSL
                     VIASPPQAEFGGLPHPTILAAAALLGVDEVWAVGGAQSIALLAYGGTDTDGTELAAVD
                     MITGPGNIYVTAAKRICRSQVGIDAEAGPTEIAILADHTADPVHVAADLISQAEHDEM
                     AASVLVTDSVQLADATDRALAHQLETTVHRERVTAALTGKQSAIVLVDDLAAGVRTVN
                     AYAAEHLEIQTVDARDVASRIRSAGAIFVGAYSPVSLGDYCAGSNHVLPTAGCARHSS
                     GLSVQTFLKGIHVVDYDEAALKDVSGYVITLSQAENLPAHGEAVRRRFER"
     gene            1761..2867
                     /locus_tag="VFC01_10715"
     CDS             1761..2867
                     /locus_tag="VFC01_10715"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZN80144.1"
                     /translation="MTVGKKVTLADLPLRQNLRGKSPYGAPQLDVPVRLNTNENPHPP
                     TKALVDDVTRSVQAVAGDLHRYPDRDAVALRTDLAAYMTGQTGTDLSVENLWAANGSN
                     EILQQLLQTFGGPGRSAIGFVPSYSMHPIISGGTQTEWLVANRADDFSLDTDVAAHAI
                     KEKSPDIVFVASPNNPSGQSVPLDELGGLLDAMTTGILILDEAYGEFSSQPSAVKLID
                     DYPTKLVVSRTMSKAFAFAGGRLGYLIAAPAVIDAMLLVRLPYHLSSVTQAAARAALR
                     HADDTLGSVAKLIAERDRVTEALTGMGFRVIPSDANFVLFGEFADAPATWQRYLDEGI
                     LIRDVGIPGYLRATTGLAEENDALLAASARIGAQ"
     gene            2864..3478
                     /gene="hisB"
                     /locus_tag="VFC01_10720"
     CDS             2864..3478
                     /gene="hisB"
                     /locus_tag="VFC01_10720"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZN80145.1"
                     /translation="MTDNRRARIERKTKESDIVVDLDLDGTGVVDVSTGVPFYDHMLT
                     SLGSHASFDLSIKATGDVDIEGHHTIEDTAIVFGQALAKALGDKKGIRRFGDAWIPMD
                     ETLAHAAVDVSGRPYCVHTGEPEHLLHTTIAGSEVPYHTVVNRHVFETIAMNARIALH
                     VRTLYGRDPHHITEAQYKAVARALRQAVEYDPRVSGVPSTKGTL"
     gene            3475..4095
                     /gene="hisH"
                     /locus_tag="VFC01_10725"
     CDS             3475..4095
                     /gene="hisH"
                     /locus_tag="VFC01_10725"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZN80146.1"
                     /translation="MTKKVVVLDYGSGNLRSAQRALERVGADVEVTSDMGAADAADGL
                     VVPGVGAFEACMTGLRKISGDKIIADRVAAGRPVLGVCVGMQILFARGVEFGLETTGC
                     GQWPGAVIRLDAPVIPHMGWNVVDAPSDSTLFKGIDASTRFYFVHSYAAQQWEGTKDA
                     LLTWATHHVPFLAAVEDGPLSATQFHPEKSGDAGAALLSNWVGALS"
     gene            4092..4835
                     /gene="priA"
                     /locus_tag="VFC01_10730"
     CDS             4092..4835
                     /gene="priA"
                     /locus_tag="VFC01_10730"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HZN80147.1"
                     /translation="MTDVRLILLPAVDVVDGKAVRLVQGQAGSETDYGSALDAAMTWQ
                     RDGAEWIHLVDLDAAFGRGSNRELLAEVVGKLDVAVELSGGIRDDASLTAALATGCAR
                     VNLGTAALENPQWCAKVVAEHGEKIAVGLDVKIVDGEHRLRGRGWETDGGDLWDVLER
                     LDREGCSRFVVTDVTKDGTLNGPNLELLSKVAERTDAPVIASGGVSSLDDLRAIATLV
                     DRGVEGAIVGKALYAGRFTLPQALAAVAQ"
     gene            4843..5658
                     /locus_tag="VFC01_10735"
     CDS             4843..5658
                     /locus_tag="VFC01_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HZN80148.1"
                     /translation="MALDETDLDALVSEAAAILDTAAKPFIDGHRADSAVQKKGNDFA
                     TEVDLAIERQVVEALNEKTGIEVHGEEFGGADVDSPLVWVLDPIDGTFNYAAGSPMAA
                     ILLGLVRDGEPVAGLTWVPFTGERFTAVVGKPLRSNGVEQPPLQTTKLADSIVGVSTF
                     NVDSRGRVPGRYRLAVIESLSRKCSRMRMHGATGIDLAYAAAGILGGAISFGAHVWDH
                     AAGVALVRAAGGIVTDLDGSDWTTKSRSALVAAPGVHGELLELLNSVGDLGNY"
     gene            5658..6440
                     /gene="hisF"
                     /locus_tag="VFC01_10740"
     CDS             5658..6440
                     /gene="hisF"
                     /locus_tag="VFC01_10740"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZN80149.1"
                     /translation="MDPKDLAVRIIPCLDVDDGRVVKGVNFENLRDAGDPVELAARYD
                     EEGADELTFLDVTASSSGRATMLEVVKHTAEQVFIPLTVGGGVRSVADVDALLRAGAD
                     KVSVNTAAIARPDLLAELSRQFGSQCIVLSVDARTVPEGSDPTPSGWEVTTHGGRRGT
                     GIDAIEWATRGAELGVGEILLNSMDYDGTKAGFDLKMLRAVRGAVSVPVIASGGAGAV
                     EHFAPAVVAGADAVLAASVFHFRELTIGEVKAAMAAEGIVVR"
     gene            6437..6784
                     /gene="hisI"
                     /locus_tag="VFC01_10745"
     CDS             6437..6784
                     /gene="hisI"
                     /locus_tag="VFC01_10745"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HZN80150.1"
                     /translation="MTLDAGIASRLKRDANGLVTAVAQERGTGQVLMVAWMDDIALAR
                     TLETRKATYYSRSRQEHWVKGETSGHTQYVHSVRLDCDGDTVLLEVDQVGAACHTGDH
                     TCFDNDLLLGPEE"
     gene            6909..7703
                     /locus_tag="VFC01_10750"
     CDS             6909..7703
                     /locus_tag="VFC01_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80151.1"
                     /translation="MSRFADRVAVVTGASSGIGRATALRLAQEGADVALVALPGADLD
                     AAAQECRATGSAALAVPTDVADSRQVAGAFDRAETLGPVSAVFSNAGISIVGPATTLD
                     DDGWLHQLHVNLSGNFYVVREAARRMVPRDYGAIVTTGSELGLLGQPGYVGYTATKGG
                     VLAMTRAFAAELAGHGIRVNSISPGTTETPMLLAEFAAAPDPARERAENESTIPLGRI
                     GQPGDIAAAVAFLLSDEAAYITGANLVVDGGRTSCFTIGTLGTLTP"
     gene            7700..8362
                     /locus_tag="VFC01_10755"
     CDS             7700..8362
                     /locus_tag="VFC01_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZN80152.1"
                     /translation="MTLPVAVGSGGAELLGDLDVPPKSTGLVVFAHGTGSSRFSPRNR
                     FVAQVLRDFGFATLLLDLLTESEEQTDLRTREFRFDVGLLADRVLSALDRMREHDAVS
                     GLPVGLFGASTGAAAALIAAAGDESVRAVVSRGGRPDLAGDALHHVEAPTLMIVGGRD
                     PRILEANRKASGELAAPHRITVIPGATHLFEEPGALEKVAALAAGWFGQHLAGLGHGD
                     GV"
     gene            8346..8615
                     /locus_tag="VFC01_10760"
     CDS             8346..8615
                     /locus_tag="VFC01_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80153.1"
                     /translation="MAMVSERLRRRIERDFPERGSASSVIELVGSIGDSERVQAAVVV
                     WGAGNLDRVRDAVELAERDWRDVLVRADLADGDWASRLDVELGPS"
     gene            complement(8612..9409)
                     /locus_tag="VFC01_10765"
     CDS             complement(8612..9409)
                     /locus_tag="VFC01_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN80154.1"
                     /translation="MTPVLELADVTFRRDGKQIIDGISLTVQEGEHWALLGPNGAGKS
                     TLLGFCAAVTFPTSGTVRILGAQMGRVDLAQLRLAIGHVNPRHRLQYPLTVREVVLTG
                     ITATIDIPNRWTPSTDELRRADDMISTVGLSRRADDVWPTLSHGERGRTLIARALISD
                     PRLLLLDEPTTGLDVAAREQLLETIDTLDETHPEVASILVTHHLEELPTTTTHALLIA
                     EGRTVAAGPARDTVTTENVSAAFSHPVEVRYDEGRWVARTKATRIDV"
     gene            complement(9406..9870)
                     /locus_tag="VFC01_10770"
     CDS             complement(9406..9870)
                     /locus_tag="VFC01_10770"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZN80155.1"
                     /translation="MKRGDQVSEFELPDQTGTVRSLTELLANGPIVLFFYPAAMTPGC
                     TKEACHFRDLASEFAAVGASRVGISADAVEKQAKFADQQKFDYPLLSDTEGTVATQFG
                     VKRGLLGKLMPVKRTTFVIDTDRTVLDVISSEFSMDTHADKALETLRQRQSA"
     gene            9873..11447
                     /locus_tag="VFC01_10775"
     CDS             9873..11447
                     /locus_tag="VFC01_10775"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HZN80156.1"
                     /translation="MSRYAGRFGLVWEDCSVQTTANLAITTSREDFRALAAEHRVVPV
                     TRKVLADSETPLSAYRKLAANRPGTFLLESAENGRSWSRWSFIGAGAPSALTVRDGEA
                     GWLGTTPQDAPRGGDPLQALRATLELLKTEPLPGLPPLSSGLVGFFAYDMVRRLERLP
                     SLAVDDLGLPDMLLLLATDIAAVDHHEGTITLIANAVNWNGTEERVDWAYDDAVKRLD
                     VMTAALSEHLPSTVATFSRPQPLHRSQRTVDEYTRIVEKLVGDIEAGEAFQVVPSQRF
                     EMDTDADPLDVYRMLRVSNPSPYMYLLNVPDATGGLDFSIVGSSPEALVTVKDGRAST
                     HPIAGTRWRGQTEEEDVLLEKELLHDDKERAEHLMLVDLGRNDLGRVCRPGTVRVEDY
                     SHIERYSHVMHIVSTVHGELADDKTALDAVTACFPAGTLSGAPKVRAMELIEEVEKTR
                     RGLYGGVLGYLDFAGNADFAIAIRTALMRNGTAYVQAGGGVVADSNGPYEYTEAANKA
                     KAVLNAIAAAETLTEP"
     gene            11444..12022
                     /locus_tag="VFC01_10780"
     CDS             11444..12022
                     /locus_tag="VFC01_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02234 family membrane protein"
                     /protein_id="HZN80157.1"
                     /translation="MIRISQLLLVLSAGALWVASRMVWVEVQSFDGLGQPKATALNGA
                     SWSTALVPLAVLLVAAALAALAVRGWPLRLLAVLVAAASAGMAYLAISLWVIRDVAVR
                     AAHLGDVPLTNLVGTQRHYGGAIVTLVAAVVTLVGAVLLVRSAARATPEPTRYERRPA
                     PSPQEAAAPSERAIWDALDEGRDPTDPDNKGR"
     gene            12094..12912
                     /gene="trpC"
                     /locus_tag="VFC01_10785"
     CDS             12094..12912
                     /gene="trpC"
                     /locus_tag="VFC01_10785"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZN80158.1"
                     /translation="MSSATVLDSIIEGVRADVAAREAVVPLAEIKEMAKTAPPPLDVL
                     AALREPGIGVIAEVKRASPSRGELASISDPAKLARAYEDGGARVISVLTEQRRFNGSL
                     EDLDAVRAAVSIPVLRKDFIVRPYQIHEARAHGADMLLLIVAALEQSVLEPMLDRTES
                     LGMTALVEVHTEEEADRALQAGAKVIGVNARDLKTLDVDRDCFARIAPGLPSNVIRIA
                     ESGIRGTADLLAYAGAGADAVLVGEGLVTSGDPRSAVADLVTAGTHPSCPKPAR"
     gene            12916..14175
                     /gene="trpB"
                     /locus_tag="VFC01_10790"
                     /pseudo
     CDS             12916..14175
                     /gene="trpB"
                     /locus_tag="VFC01_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560443.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
     gene            14172..14960
                     /gene="trpA"
                     /locus_tag="VFC01_10795"
     CDS             14172..14960
                     /gene="trpA"
                     /locus_tag="VFC01_10795"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZN80159.1"
                     /translation="MSRLASLFEDCGAEGRSALIGYLPTGFPDVPTSISAMTALVESG
                     CDIIEVGIAYSDPGMDGPVIAAATEAALRGGVRVRDALAAVEAISKAGGRAVVMTYWN
                     PVLRWGVDAFARDLASAGGLGMITPDLIPDEADEWLAASETHNLDRIFLVAPSSTPER
                     LAATVKASRGFVYAASTMGVTGARDAVSVAAPELVRRVKAVSDIPVGVGLGVRSREQA
                     AEIGAYADGVIVGSALVSALKDGIPAVRSLTEELAGGVRQRITA"
     gene            14957..16852
                     /gene="lgt"
                     /locus_tag="VFC01_10800"
     CDS             14957..16852
                     /gene="lgt"
                     /locus_tag="VFC01_10800"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZN80160.1"
                     /translation="MTTTYLAYIPSPPQGVWHLSFIPIRAYALFIVAGIVVALMLGDR
                     RWVARGGQAGVIYDIALLAVPFGLIGGRLYHVITDWRTYFGDDGAGLLGAFRIWDGGL
                     GIWGAVALGGVGAWIGCRYKGIPLPAFGDAIAPGIILAQAIGRVGNYFNQELYGRPTT
                     LPWGLEIYERVNANGAPDNLNGVSTGELLNIYHPTFLYELLWNVLIFAALIYIDRRFK
                     IGHGRLFAMYVAGYCFGRFWIELMRSDYASQPFDGIRVNSLTSTFVFIGAVVYIMVAP
                     KGREDPASLAGKPEEDEDTVETFAKELTAVAATTGVVAAAKAAGDAEDEDAKDAVEDE
                     KPEEPDEPAVKDAEAFMTDVPAESVEEAEEFAAEGEAEPEAGLGEVEGEEAVEALAAA
                     PEAHEAAVEAAEEAEAVADEVAAEDEAEEPEAEAEEPEAETEDTTDVQPEAESAEQPE
                     AETAEPEAGLGAVEGEEAVEALEAAPDVHDAAVEAADEAEAVADEVAAEEEPSSEAAA
                     EAGALAEDVAEAPSEDIAEAEEFAEAGEEAEQAEAAAESEESEPDEPETEDAEAAVEE
                     AEAEPSVEEAAEEAEAVVEEADSAAEDEPTTGVLEAKTEPEQTPEPAAPTEETGGRLR
                     RWLRRDR"
     gene            complement(16968..18290)
                     /locus_tag="VFC01_10805"
     CDS             complement(16968..18290)
                     /locus_tag="VFC01_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN80161.1"
                     /translation="MFSETRYALNGDLRVAYRASREGPRDLVFVSNWFTNCEVAPELP
                     SVQGWVEAMMSLGRLILFDQPGTGASDPITPGALPTLEQWADSITAVLDDLGSREAVL
                     LAVGGAIATGALFAATHPSRTTALVVLEGYADDPVAQSTDGLDREGANAAIVAMWGSG
                     AFQHVVNPDMPWNEEIRATWARLERLGASPGTVALMLPLVAEVDVRAVLPTIRVPTLV
                     VQHADDRFFQCASGKYIADHIPDAKYVELPGRNAWHIVEPWRASFQEIAKFLTGHQAE
                     VADDRVLATVLFTDIVDSTRKAAEIGDRDWHALLDAHDAVVRAQLSRFRGREVNTLGD
                     GFLATFDGPQRAIRCAMAIREAVQSIGIEVRAGLHTGECEVRGDDIGGIGVHIGARVS
                     AMAGPNEVLVSSTLRDLVIGSGLEFEERGTHELKGVPGDWHLFAVASP"
     gene            complement(19339..19743)
                     /locus_tag="VFC01_10810"
     CDS             complement(19339..19743)
                     /locus_tag="VFC01_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN80162.1"
                     /translation="MTTQTEDCRAIVVKFVDAFGDQRLDDALSLLHDEFVVHAAGDVP
                     YSGDYCGPAGFLELITKISDALELTPSPQMQFLADGDKVVLHYRLRFTARASGESVEM
                     SMAEVFTVRDGLIVELDVFYKKPSAVEALLAV"
     gene            complement(20720..22189)
                     /locus_tag="VFC01_10815"
     CDS             complement(20720..22189)
                     /locus_tag="VFC01_10815"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018178400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN80163.1"
                     /translation="MTGRQPRIVIIGAGIAGIATAVTLQREGFHDFTILEKGADVGGV
                     WHWNRYPGLTCDVPSQLYQFSFAPKPDWTSLFASGEEIQRYLRDVVERFGLDDHLRLN
                     AEVVSAVFTGSGWQIRTSDGGELKADFVIAATGVLHHPFTPDIPGLDTFRGDVVHTAR
                     WDDDVVTSGRKIAVIGTGSTGVQVVSALQRGAKHITHFVRTPQWVMWAPMGLPQPAIL
                     GAALRRLPGVHRWMYGVLLWASGILADVTTRPSWRRRLVQDFARWSLRTQVRDRELRS
                     RLTPDYQPLCKRQVVSASYYRALRARNAELVTDAIERITPRGIRTADGREIDVDVLVF
                     ATGFQAHNYMRPMNLRGRDGVSIDDAWAKGPRAYRMSAIPGFPNFFTVLGPNSPTGSI
                     SLQYSAELTARFITQWLRRFRDGEIDTVEVTEEATTKFNDDVAAALGPTVWNTGCNSW
                     YLTDEGNVDLWPYDRKTMTAMLAQPDDRHYHVTRAVPAT"
     gene            complement(22186..23001)
                     /locus_tag="VFC01_10820"
     CDS             complement(22186..23001)
                     /locus_tag="VFC01_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN80164.1"
                     /translation="MMSYPKIELDGAVVAVTGGARGIGRATAELFAAKGATVGVGDLD
                     GGNYPLDVTSRESFAEFTAAVLARFGRIDVLVNNAGVMPLGDFLSEDDATSRTTVDVN
                     VWGLIHGMRLVMPHMIERGSGHVVNVASMAGKLVVPGMAVYNASKFAAVGLSAAVREE
                     YRDTGVSVSAVLPSAVRTRLASGVPLGHGMPTVDPEDVARAIVGSVDSRRAEITVPRY
                     LAGWDLVNAAVPERLMRLGRKLIGDRRALTSIEHDVREEYEQTIRGQAPEAQS"
     gene            23073..23654
                     /locus_tag="VFC01_10825"
     CDS             23073..23654
                     /locus_tag="VFC01_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80165.1"
                     /translation="MARKSRAETQAETRAQLVKTARQLFFEDGYHPTSLEKVADAAGY
                     SKGAVYSNFRNKDELCTAVLDEVRAERLGEVVEIFGKPDTPSRIDAIRDWAERVIGDP
                     GWTTLEVEFAAHARPNEQLRTELAGRLDGILQMLTAATESADTADLKMPAREASTILL
                     ALGVGLGLLRSIDPAIPVSGLVDTLRLLSGQSL"
     gene            23719..24147
                     /locus_tag="VFC01_10830"
     CDS             23719..24147
                     /locus_tag="VFC01_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HZN80166.1"
                     /translation="MTEPQFSGSESGNPYPPPPQQPGYPPPAGQYPPPPGQYPPPPGP
                     YPPAYVDPAAPYGRHPLTGEPLSDKSKLVAGLLQLIGLFGLVGIGRIYLGYTSLGVVQ
                     LVVGLITCGIGAVVWGIVDAVLLFTDKVRDPEGRPLRDGT"
     gene            24137..24544
                     /locus_tag="VFC01_10835"
     CDS             24137..24544
                     /locus_tag="VFC01_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HZN80167.1"
                     /translation="MAPSATTRRSLYGALGTGAVLVGGLAYVGLGDPHSPGFVFPVCP
                     FKALTGWNCPACGGLRMTHDVLHGDLGAAVVDNVFLLVGLPLLAAWLLVRWRRGQSLM
                     PMPAIVVIAMAAITWTVVRNLPGFPLVPTLLTG"
     gene            24628..>27304
                     /locus_tag="VFC01_10840"
     CDS             24628..>27304
                     /locus_tag="VFC01_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HZN80168.1"
                     /translation="MLFSALPDRQGLYDPENESDSCGVAMVTDIQGRRSHAIVADGLI
                     ALEHLEHRGAAGAEPNSGDGAGILIQLPVELLKEVVDFELPAPADDGCNTFAAGICFM
                     PQDPDDRAKAREHVEAIAKEEGLHVLGWREVPVDPHGAEVGVTALSCMPYMTQLFVSA
                     PGQSGIDLDRRIYPLRKRAERNGVYFPSLSSRTMVYKGMLTTMQLPQYFPDLRDERCM
                     SAIAIVHSRFSTNTFPSWPLAHPFRFVAHNGEINTVRGNRNRMHAREAMLASAKIPGD
                     LARLSPICTPEASDSASFDEVLELLHLGGRSLPHAVLMMIPEAWENNSTMDPAERAFW
                     QFHASLMEPWDGPACVTFTDGTLVGAVLDRNGLRPGRWWRTIDDRVILASESGVLDVP
                     SAEIVAKGRLQPGKMFLIDTAQGRIVADDEIKDNLSKQEPYGEWLHAGLLDLKTLPDR
                     VRVQPNHESVVRRQISFGYTEEDLRVLLTPMAASGAEPLGSMGTDTPTAVLSQRSRLL
                     YDYFVELFAQVTNPPLDAIREEVVTSMARVMGPEQNLLAPTAASCRQIVLRWPVLDND
                     ELNKIVHINDDREHPGLKTAVLKALHDVERGGEGLADALDDLQQRACDAIAKGARTLV
                     ISDRDSDHTRAPIPSLLAISAVHHHLVRTKQRTTVALVVESGDAREVHHIAMLIGFGA
                     AAVNPYLAFESIDDLIREGELTGIEPAAAVRNYVKALGKGVVKVMSKMGISTVASYTA
                     AQAFEAVGINKDVIDEYFTGTPCQLGGAGLDVIAEEVKQRHRRAYPENPTERVHRRLE
                     VGGEYAFRREGELHLFTPEVVFLLQHSTRTGRHDIFGQYSDEVNRLSKEGGTLRGLFD
                     FKKLRPPVPLEDVEPVDSIVTRFNTGAMSYGSISAA"
BASE COUNT         4754 a         8847 c         9070 g         4633 t
ORIGIN      
        1 tggcaaacat tattgtcaaa tattcacgca gtccagcaat cgcttcagaa ttcagttggc
       61 aacgctggcg agcgcggcgg ttcaccgctg acgacgctgc agaatctcat taccgttatt
      121 gacggctatt aatacagcat gtagcggcgt cgaacttcga gaccagttgc gtgtgcaaat
      181 aaaactgcga aaaagcgagc cgccgatagt tggcaaatag tgcctgcaac gtccgatgac
      241 attgtggcaa agaagagcgt ggccatcgac gctcttggat cgattcgaac cggggagtct
      301 gagtgcggac atcgagcttt gctgatggaa cggcagctgt gcgcgacggc gggtggtacc
      361 tgaccggcgg gctttccctg cgcccacgcc tttatcgatt tctttcggct gggcgcatcg
      421 gggacaatgg gcgtgatggc cgatttccag atgacccgca tcgacctgcg caaccgctcc
      481 ctgaacgcgg cgcagctgcg cactgcgctg cctcgcggcg gggtcgacgt cgacgccgtg
      541 gtgccaaagg tgcgcccgat cgtcgacgcg gtcgccgaaa acggcgcgca ggccgcgttg
      601 cggtatggcg aatcgttcga cggcgtgcgg cccgctacgg tccgagtgcc ggtcggcgag
      661 ctggacgccg cactgaacgc cctcgaggcc gacgtccgcg tcgcactgca ggtggccatc
      721 gaccgggccc gggctgtgca tgccgaccag cggcgcaccg acaccacgac caccctcgcg
      781 ccgggcgcaa ccgtcaccga gcgctgggtg cccgtcgagc gggtcggcct ctacgtgcct
      841 ggcggcaacg ccgtctaccc gtccagcgtg gtgatgaatg tcgtgccggc gcagaccgcc
      901 ggtgtggact cgctggtgat cgccagcccg ccgcaggccg aattcggcgg cctgccgcac
      961 ccgacgatcc tggcagccgc cgcattgctt ggcgtcgatg aggtgtgggc ggtcggcggt
     1021 gcgcagtcca tcgcgctgtt ggcctatggc ggaaccgaca cagacggcac cgagctcgca
     1081 gccgtcgaca tgatcaccgg accgggaaac atctacgtca ccgcagccaa gcggatctgc
     1141 cggtcgcagg tcggcatcga cgcggaggcc ggtccgacgg agatcgcgat ccttgccgac
     1201 cacaccgcgg acccggtaca cgtcgccgcc gatctgatca gccaggccga gcacgacgag
     1261 atggccgcca gcgtgctcgt caccgacagc gtgcaactgg ccgacgccac ggaccgcgca
     1321 cttgctcacc agctggagac gacggtgcac cgcgagcggg tgaccgcggc gctgacgggc
     1381 aagcagtcgg cgatcgtgct cgtcgacgac ctcgccgccg gtgtgcgcac cgtcaacgcc
     1441 tatgccgccg agcacctgga gatccagaca gtcgatgcac gcgacgtggc aagccgaatc
     1501 cgttcggccg gtgcgatttt cgtgggcgcc tattcaccgg tcagcctcgg cgattactgc
     1561 gcaggatcga atcacgtgct gcccaccgcg ggctgtgcgc gccactccag cggactgtcg
     1621 gtgcagacct tcctgaaggg catccacgtc gtcgactacg acgaggcagc gctgaaagac
     1681 gtgtctggat acgtgatcac gctgtcgcag gccgagaacc tgcccgccca cggcgaggcg
     1741 gtcaggcgga ggtttgaaag gtgacggttg gcaagaaggt gacgctggcc gatctgccgc
     1801 tgcgtcagaa cctgcgtggc aaatcaccat atggtgcacc gcaattggac gttccggtgc
     1861 ggctcaacac caacgagaac ccgcatcccc ccacaaaggc tctcgtggac gacgtgaccc
     1921 gctcggtcca agcggtggct ggtgacctgc accgctatcc cgaccgcgac gcggtcgcat
     1981 tgcgcacgga tctggccgca tacatgaccg ggcagaccgg caccgacttg tctgtcgaaa
     2041 acctctgggc cgccaacggt tccaacgaga ttctgcagca gctgttgcag acattcggcg
     2101 gtccgggccg cagcgcaatc ggcttcgtgc cgtcctattc gatgcacccg atcatctccg
     2161 gcggcaccca gaccgagtgg ctggtcgcca atcgcgccga cgacttcagc cttgacacgg
     2221 atgtcgcagc gcacgcgatc aaagagaaat cccccgacat cgtattcgtc gcgagcccga
     2281 acaacccgtc gggccaaagc gttccgctcg acgagttggg tgggctgctg gatgcgatga
     2341 cgaccgggat tctgatcctc gacgaggcct acggcgagtt ctcgtcgcag cccagcgcgg
     2401 tcaagctgat cgacgactac ccgaccaaac ttgtcgtcag ccgcacgatg agcaaggcgt
     2461 tcgcgttcgc cggtggtcgg ctcgggtact tgatcgccgc gccggcagtg atcgacgcga
     2521 tgctgctggt gcgcctgccg tatcacctgt catctgtcac ccaagcggcc gcgcgcgccg
     2581 cgctgcgcca cgccgatgac acgctgggca gtgtcgccaa gctcatcgcc gaacgcgacc
     2641 gcgtcacaga agcgttgacc ggcatgggtt tccgggtgat tcccagcgac gccaacttcg
     2701 tgttgttcgg cgaattcgcc gatgcacctg ccacctggca gcgctatctc gacgagggca
     2761 tcctgatccg cgacgtgggc attcccggat acctgcgcgc gaccaccggc ctggccgagg
     2821 aaaacgacgc gctgctggcc gccagcgccc gaataggagc acagtgaccg acaaccgtcg
     2881 cgcgagaatc gagcgcaaga ccaaggaatc cgacatcgtc gtcgacctcg acctcgacgg
     2941 caccggcgtc gtcgacgtca gcaccggcgt gccgttctac gaccacatgc tgacttcgct
     3001 gggcagccac gccagcttcg atttgagcat caaggcgacg ggcgatgtcg acatcgaggg
     3061 ccatcacacc atcgaggaca ccgcgatcgt gttcggccag gcgctggcaa aagcgctggg
     3121 ggacaagaag ggcatccggc ggttcggcga cgcgtggatc ccgatggacg agacgcttgc
     3181 ccacgccgcc gtcgacgtgt caggccggcc gtactgcgtg cacaccggcg agccggagca
     3241 cctgctgcac accacaattg cgggctccga ggtgccgtat cacaccgtcg tcaaccggca
     3301 cgtgttcgag acgatcgcga tgaacgcccg tatcgcgttg catgtgcgca cgctctacgg
     3361 ccgcgaccca caccacatca ccgaggcgca gtacaaggct gtcgcccgtg cgctgcgcca
     3421 ggcggtcgag tacgacccgc gggtcagcgg cgtgccgtcc accaaaggca cgctgtgaca
     3481 aagaaagtcg tcgtcctaga ctacggctcc ggcaacctgc gctcggccca gcgcgcgctg
     3541 gagcgagtcg gcgcggacgt tgaggtgacc tccgacatgg gagccgccga tgcagccgac
     3601 ggccttgtcg tgcccggtgt cggcgcattc gaggcgtgca tgaccggtct gcgaaagatc
     3661 agcggggaca agatcatcgc cgatcgggtc gcggcgggtc gaccggtgct cggtgtgtgc
     3721 gtcggtatgc agattctgtt cgcccgcggc gtggagttcg gcctcgagac caccggttgc
     3781 gggcagtggc cgggcgctgt catccggctg gatgcgccgg tgattccgca catgggctgg
     3841 aacgtcgtcg acgcgccctc cgacagcacg ctgttcaagg gcatcgacgc cagcacccgg
     3901 ttctacttcg tgcactccta tgccgcgcag cagtgggagg gcaccaagga cgcgctgctg
     3961 acctgggcca cccatcacgt cccattcctg gccgcggtcg aggacggacc cttgtcggcg
     4021 acccagttcc atccggaaaa aagtggcgac gccggtgccg cattgctcag caattgggtc
     4081 ggagcattaa gttgaccgac gtgcgtttga ttctgttgcc tgccgtcgac gttgtggacg
     4141 gcaaggctgt ccgcctggtt caaggccagg ccggcagcga aaccgactac ggctccgccc
     4201 tggacgccgc catgacgtgg cagcgtgacg gcgccgagtg gatccacctc gtcgacctgg
     4261 acgccgcatt cggccgcggc tccaatcggg aactattggc cgaagtggtc ggcaagctcg
     4321 acgtcgcggt cgaactgtcg ggcggcatcc gcgacgacgc atcgctcacg gcggcgctgg
     4381 ccaccggctg cgcacgtgtg aacctcggca ccgcggcgct ggagaacccg cagtggtgcg
     4441 ccaaggtggt cgccgagcac ggcgagaaga ttgcggtcgg tctcgacgtg aagatcgtgg
     4501 acggcgagca ccggctgcgc ggccgcggct gggagacaga cggtggcgac ttgtgggatg
     4561 tgctggaacg ccttgaccgt gagggctgtt cgcgcttcgt cgtcaccgac gtgaccaagg
     4621 acggcaccct gaacgggccc aacctcgaac tgctcagcaa ggtcgccgag cgcaccgacg
     4681 ccccggtgat cgcctccggc ggggtatcca gcctggatga ccttcgcgcc atcgccacgc
     4741 tggtcgatcg cggcgtcgaa ggcgccatcg tcgggaaagc gttgtacgcc ggacgattca
     4801 cgctgccgca ggcgctggcc gcggtggcgc agtaggacat cgatggcgct cgacgagacc
     4861 gatctggacg cgctggtctc cgaggccgcc gcaatcctgg acaccgccgc gaagccgttc
     4921 atcgacggcc accgcgccga ttcggcggtg cagaagaagg gcaacgactt cgccaccgag
     4981 gtcgacctgg ccatcgaacg ccaggttgtc gaggcgctca acgaaaagac cgggatcgag
     5041 gtgcacggcg aagaattcgg cggcgccgac gtcgattcac cgctcgtgtg ggtgctcgac
     5101 ccgatcgacg gcactttcaa ttatgccgca ggctcaccga tggcggccat cctgctcggt
     5161 ctggtccgcg acggtgagcc ggtcgcgggg ctgacgtggg tcccgttcac cggcgaacgg
     5221 tttactgctg tcgtcggcaa accgttgcgc agcaacggtg tcgagcaacc cccgttgcag
     5281 acgacgaaat tggccgactc catcgtcggc gtcagcacct tcaacgtcga ctcgcgggga
     5341 cgagtgcccg gccgctaccg gctggcggtg atcgaaagcc tgagccgcaa gtgctcccga
     5401 atgagaatgc acggcgcaac gggtatcgat ctcgcgtacg ccgcagccgg aatcctcggc
     5461 ggggcaatca gtttcggtgc ccacgtgtgg gaccatgcgg cgggcgtcgc gctggtgcgg
     5521 gcggccggcg gcatcgtcac ggacctggat ggcagtgact ggaccaccaa atcgcgctcg
     5581 gcgttggtcg cggcacccgg cgtgcacggc gagcttctcg agctcctgaa ctccgtcggc
     5641 gatctgggga attactgatg gaccccaaag atcttgcagt gcgcatcatc ccgtgcctgg
     5701 acgtcgacga cggcagggtg gtcaagggcg tcaacttcga aaacctgcgc gatgcaggcg
     5761 atccggttga actggccgct cgctatgacg aggagggcgc cgacgagctg acgttcctcg
     5821 acgtcaccgc ttcgtcatcg ggccgggcta ccatgctgga ggtcgtcaag cacaccgcag
     5881 agcaagtgtt catcccgctc accgtcggcg gtggcgtgcg ctcggtcgcg gatgtcgacg
     5941 cgctgcttcg ggccggcgcc gacaaggttt cggtcaacac agccgcaata gcccggcccg
     6001 atctgcttgc agagctgtcc cgtcaattcg gatcgcaatg catcgtgctg tcagtcgacg
     6061 cgcgtacggt ccccgagggc tccgatccga cgccgtcggg ctgggaggtg accacgcacg
     6121 gcggcaggcg gggcaccggg atcgacgcga tcgaatgggc cacccgcgga gccgaactcg
     6181 gcgtggggga gatcctcctc aactccatgg actatgacgg caccaaggcc ggtttcgacc
     6241 tgaagatgct gcgggccgtg cgcggcgcgg tgtcggtgcc ggtgatcgcc agcggcggcg
     6301 cgggcgcggt ggaacatttc gcacccgccg ttgttgcagg agccgacgcc gtgctggccg
     6361 ccagcgtgtt ccacttccgg gagctgacca tcggcgaggt caaggctgcg atggcggcag
     6421 aagggatcgt ggtgagatga cgctcgacgc tggtatcgcg tcgcggctga agcgcgatgc
     6481 caacgggctg gtcaccgcgg tcgcgcagga gcgcggcacc ggccaggtgc tgatggtggc
     6541 gtggatggac gacatcgcgc tggcgcgcac gctggaaacc cgcaaggcga cctactattc
     6601 gcggtcgcgc caagagcatt gggtcaaggg tgagacatcg ggccacaccc agtacgtgca
     6661 ctcggtgcgg ctggactgcg acggcgacac cgttctgctg gaggtcgacc aggtcggcgc
     6721 cgcctgccac accggcgacc acacttgttt cgataacgac ctgctactgg ggccggagga
     6781 gtagcggcgg atcgccatga cgaacgttac ttaccgctcg acatttggcg attttttcga
     6841 gcggtaacga acgttcggct atagcgatct gcactgcacg ccagcgcgcg actcgcgaca
     6901 ctagacggat gtctcgcttc gccgatcgcg tcgccgttgt caccggcgcg agttctggaa
     6961 ttggtcgcgc caccgcgctt cggctcgcgc aggagggcgc ggacgtcgcg ctcgtcgccc
     7021 tccccggcgc cgatctggat gcagccgcac aggaatgtcg cgccactgga tctgccgcgc
     7081 tcgccgtgcc gaccgacgtc gcggactctc gccaggtggc aggtgcattc gatcgtgccg
     7141 aaaccctcgg ccctgtttcc gcggtcttca gcaatgccgg catctccatc gtcggacccg
     7201 caacgacact tgatgacgac ggctggctgc atcagctcca tgtcaacctc tccgggaatt
     7261 tctatgtcgt gcgcgaagcc gctcgccgca tggttccgcg ggattacggc gccatcgtca
     7321 ccaccgggtc agaactcggg ctcctcggcc agccgggtta cgtcggctac acggcgacga
     7381 agggcggggt gctggcgatg acgcgggcct tcgccgcgga gctggccgga cacggaatcc
     7441 gcgtgaacag catttccccg ggcaccaccg aaacgccgat gctgcttgcc gaattcgccg
     7501 ccgcgcccga cccggcgagg gagcgggcgg agaacgagtc gaccatcccg ctcggccgca
     7561 tcggacaacc gggcgacatc gcggcggccg tcgcgttcct gttgtccgac gaagccgcct
     7621 acatcaccgg cgccaacctc gtcgtcgacg gcggtcgcac ctcgtgtttc acgatcggga
     7681 ccctgggcac tctgactcca tgacattgcc ggtcgccgtc gggtcgggcg gcgccgaatt
     7741 gctcggcgat ctcgacgtcc ctccgaagtc cactggactc gtcgtgttcg cgcacggcac
     7801 gggcagttcc cgcttcagcc cccgcaatcg ttttgtggca caggtgctca gagacttcgg
     7861 tttcgccacc ctgctactgg acctgctcac cgagagcgaa gagcagacgg atctacgtac
     7921 ccgcgaattt aggttcgacg tcgggctgct ggccgaccgt gtgctatcgg cgctcgaccg
     7981 aatgcgcgaa cacgacgcgg tcagcggcct cccggtgggg ctgttcggcg cgagcaccgg
     8041 cgctgccgcg gcgctgatcg ccgccgccgg ggacgaatcg gtgcgtgccg tcgtatcccg
     8101 cgggggcaga cctgatctcg cgggcgacgc cctgcaccac gtcgaggcac cgacgctgat
     8161 gatcgtcggc ggccgcgacc cccggattct cgaggccaac cggaaggcca gcggcgaatt
     8221 ggctgcgccg caccgcatca cggtgattcc gggcgctacc catctattcg aagagccggg
     8281 cgcgctcgaa aaagttgccg cgcttgccgc gggctggttc ggccagcatc tggcagggtt
     8341 gggtcatggc gatggtgtct gagcgtctcc gtcggcggat cgagcgcgac tttccggagc
     8401 ggggaagcgc gtcgtcagtc atcgaattgg tcggctcgat cggtgactcc gagcgggttc
     8461 aagcggccgt cgtggtgtgg ggtgccggca atctcgatcg ggtgcgcgat gccgtcgaac
     8521 tggctgaacg ggattggcgc gatgtcttag tgcgggctga tctggcggac ggggactggg
     8581 cctcgcggct ggacgtcgaa ctcggtccga gttagacgtc gatgcgggtg gccttcgtcc
     8641 gggcgaccca ccggccctcg tcatagcgga cctcgacggg atgtgagaaa gcggccgaga
     8701 cgttttcggt cgtcacggtg tcacgcgcag gcccggcagc gaccgtgcgg ccctcagcga
     8761 tcagcagcgc atgcgtcgtc gtggtcggca gctcttcgag gtgatgggtg accaggatcg
     8821 aggccacctc gggatgcgtt tcgtcgagcg tgtcaatggt ttccagcagc tgttcgcgcg
     8881 cggccacgtc cagccccgtg gtgggttcgt cgagcagcaa cagccgcgga tccgaaatca
     8941 gtgcgcgcgc aatgagagtg cggccgcgct cgccgtgcga cagcgttggc caaacgtcgt
     9001 ccgccctgcg ggacagcccg accgttgaga tcatgtcgtc cgcgcggcgc agttcatccg
     9061 tgctcggcgt ccagcggttc gggatgtcga tcgtcgcggt gatgcctgtt agcaccacct
     9121 cgcgcacggt caacgggtat tgcaggcggt gccgcggatt cacatgcccg atcgcaaggc
     9181 gcagctgagc caggtcaaca cgacccatct gcgcaccgag gatgcgcacc gttcccgacg
     9241 tggggaacgt gactgccgcg cagaagccga gcagcgtgct cttgcccgcc ccgttgggcc
     9301 cgagcagagc ccagtgctcg ccctcctgca cggtcagcga gatgccgtcg atgatctgct
     9361 tgccgtcgcg gcgaaacgtc acatcggcga gttccagaac cggcgtcacg ccgattgtcg
     9421 ctggcgcaat gtctccagcg ccttgtcggc gtgggtgtcc atgctgaact cgctggagat
     9481 cacgtcgagc acggtgcggt cggtgtcgat cacgaacgtg gtccgcttga ccggcatgag
     9541 cttgccgagc aggccccgct tgacgccgaa ttgcgtcgcc actgtgcctt cggtatcgga
     9601 cagcagcgga tagtcgaact tctgctggtc ggcgaacttg gcctgcttct ccaccgcatc
     9661 ggcgctgatg ccgacgcggc tggcgccgac ggcggcgaac tccgaagcca ggtcgcggaa
     9721 atggcaggcc tccttggtgc agcccggcgt catcgccgcg gggtagaaga acagcacgat
     9781 gggaccgttg gcgagcagct ctgtgaggct tcgcacagtg cccgtttggt ccggcagttc
     9841 gaactcggat acctggtcac cacgtttcat ggatgtcacg ctacgccggt cgatttggac
     9901 tggtctggga ggattgttcc gtgcaaacga ccgccaacct cgcgataacc acctcgcgtg
     9961 aggacttccg ggcgctggcc gccgagcacc gggtggtgcc ggtgacccgc aaggtgctcg
    10021 ctgacagtga gaccccgctg tcggcgtacc gcaagctggc cgccaaccgg cccgggacgt
    10081 tcttgctgga gtcggccgag aacggccgct cgtggtcgcg gtggtcgttc atcggtgccg
    10141 gggccccgtc cgcattgacg gtgcgcgacg gcgaggccgg ttggctgggc accacgccgc
    10201 aggacgcacc acgcggcggt gacccgctgc aggcgctgcg cgcgacgctg gagctgctga
    10261 agaccgagcc gctgccgggc ctgccgccgc tgtccagcgg tttggtcggg tttttcgcct
    10321 acgacatggt ccgcaggctg gagcggctgc cgtcgctcgc ggtcgacgac ctcggcctgc
    10381 cggacatgct gttgttgctg gccaccgaca tcgccgccgt cgaccaccac gaaggcacca
    10441 tcacgctgat cgcgaacgcc gtgaactgga acggcaccga ggagcgcgtc gactgggcct
    10501 acgacgacgc cgtcaagcgg ctcgacgtga tgaccgcggc attgtccgaa catctgccgt
    10561 cgacggtggc gacgttcagc aggccgcagc cgttgcaccg ctcccagcgc acggtcgacg
    10621 agtacacgcg catcgtcgag aagctcgtcg gcgacatcga ggccggcgag gcgttccagg
    10681 tggtgccgtc gcagcggttc gagatggaca ccgacgccga tccactcgac gtataccgga
    10741 tgctgcgggt gtccaacccg agcccgtaca tgtacctgct gaacgtgccc gatgccactg
    10801 ggggacttga cttttcgatc gtcggctcca gccctgaggc gttggtgacg gtcaaggacg
    10861 gtcgggcgtc gacgcatccg atcgccggca cgcgttggcg cgggcagacc gaagaagagg
    10921 acgtgctgct cgaaaaggag ctgctgcacg acgacaagga acgtgccgag catctgatgc
    10981 tggtcgacct cgggcgcaac gacctgggtc gggtatgccg gcccggcacc gtacgcgtcg
    11041 aggactacag ccacatcgag cggtacagcc acgtcatgca catcgtgtcg acggtgcacg
    11101 gcgagctcgc cgacgacaag actgcgctgg acgccgtcac cgcatgcttc ccggccggga
    11161 cgctgtcggg tgcgccgaag gtgcgggcga tggagctgat cgaggaggtc gagaagaccc
    11221 gccgcggcct gtacggcggc gtgctgggtt acctggactt cgctggcaac gccgacttcg
    11281 cgatcgcgat ccgcaccgcc ctgatgcgca acggcaccgc atatgtgcag gccggcgggg
    11341 gagtcgtggc cgactccaac ggcccgtacg agtacaccga ggcggcgaac aaggccaaag
    11401 cggtgctgaa cgcgatcgcc gcagcggaaa cactgaccga accgtgatcc ggatttccca
    11461 gctactgctc gtgctgtccg ccggggcgct gtgggtggcg tcgcggatgg tgtgggtcga
    11521 ggtgcagtcg ttcgacgggc tgggtcagcc caaggcgacg gcgttgaacg gggcgtcgtg
    11581 gtcgacggcg ctggttccgc tggcggtgct cttggtggcc gccgcgttgg cggcgctggc
    11641 ggttcgcggc tggccgctgc ggttgctcgc ggtactcgtc gccgcggcga gcgcggggat
    11701 ggcctacctg gccatcagcc tgtgggtgat ccgcgatgtc gcagtgcggg ccgcgcatct
    11761 gggcgacgta ccgctgacga acctcgtcgg cacgcagcgg cactatggcg gcgcgatcgt
    11821 cacgttggtg gcagcggtgg tgacgcttgt cggtgccgtc ttgctcgtgc gttcggcggc
    11881 cagggccacg ccggagccga cccgctatga gcgtcgtccc gcgccctcgc cgcaggaggc
    11941 cgccgcaccg tcggagcggg cgatctggga cgccctcgac gaggggcgtg acccgaccga
    12001 tccggacaac aagggtcggt gatcgatcgt gctgtggtgg cggttaccct tcgttgaaga
    12061 tcaaacgtgc tttcaggaag gaccgcggcg ggtatgagtt cggcgactgt gctcgactcc
    12121 atcatcgagg gagttcgcgc cgacgttgca gctcgtgagg ccgtcgttcc cctcgccgag
    12181 atcaaagaga tggccaagac cgcaccgccg ccgctcgacg tgctggccgc gctgcgggaa
    12241 ccgggcatcg gcgtgatcgc ggaggtgaag cgggcgagcc cgtcgcgcgg tgagctggca
    12301 tcgatttccg atccggccaa gctggcccgc gcctacgagg acggtggggc gcgcgtcatc
    12361 agcgtgctga ccgagcagcg ccggttcaac ggttcgctcg aggacctcga cgcggtccgc
    12421 gcggccgtgt caatcccggt gttgcgcaag gactttattg tccggccgta tcagatccac
    12481 gaggctcgcg cgcacggcgc cgacatgctg ctgctgatcg tcgctgcgct ggagcagtcg
    12541 gtactggaac cgatgctgga ccgcactgag tcgctgggga tgactgcgct cgtcgaggtg
    12601 cacaccgagg aggaggccga ccgcgcgttg caggcgggcg cgaaggtcat cggcgtcaat
    12661 gcacgcgacc tcaagacgct tgacgtcgat cgtgattgct tcgcacgcat cgcgccgggg
    12721 ctgccgagca atgtcattcg gatcgcggag tccgggatcc gtggcaccgc tgatctgctc
    12781 gcgtacgccg gcgcgggtgc cgacgcggtg cttgtcggtg aggggctggt caccagtggc
    12841 gatccccgca gcgccgttgc cgatttggtt accgcaggca cgcatccgtc gtgcccgaaa
    12901 cccgcgcgct gacaagtggc ggatctcgcc ggaccggagc tgccgcgttc cagcgcggcg
    12961 gtggccgaac ccagcgtcca cgatcccgat gcgcgtggcc atttcggtgt ctacggtgga
    13021 cggttcgttc cagaggcgct gatggcggtc atcgaggagg tcaccgccgc ctatgaaaag
    13081 gctcgcggcg accaagcttt cctcgacgag ctggatcggt tgcagcggca ctacagtggg
    13141 cgaccgtcgc cgttgtatga ggctgaacgg ctcagcgacc acgcgggtgg cgcacgactc
    13201 ttcctgaagc gagaagacct caaccacacc ggatctcaca agatcaacaa cgtgctcggg
    13261 caggcgttgc ttgcgcgcca gatgggcaag acacgggtga tcgctgaaac cggtgcaggg
    13321 cagcatgggg tggcgaccgc gacggcgtgc gcgctgctgg gcctcgagtg agtcgtctac
    13381 atgggcgcga tcgacaccgc gcggcaggcg ctgaacgtcg cgcggatgcg actgttgggt
    13441 gcgacggtgg tgtcggtcga ggccggctcg aagaccttga aggacgcgat caacgaggcg
    13501 ttccgtgact gggtcaccaa cgcggacaac acgtactact gcttcggcac cgcggcgggc
    13561 ccgcacccgt tcccgacgat ggtgcgcgac tttcagcgag tgatcgggct ggaagcgcgg
    13621 gtgcagatcg tcgagcaggc gggccggttg cccgatgcgg tgacggcgtg cgtgggcggc
    13681 ggatcgaacg cgatcggcat cttccacgcg ttcatcgacg atccgggtgt ccggctcgtc
    13741 ggctacgagg cggcgggtga cggtgtcgaa accgggcgtc acgcagcgac gttcactggt
    13801 ggttcgccgg gcgcatttca gggctcgttc tcatatctgc tgcaggacga ggacgggcag
    13861 acgatcgaat cgcattcgat ctcagcgggt ttggattacc caggggtcgg gccggagcat
    13921 gcgttcctga aggacaccgg tcgtgcggag taccggccca tcactgacac cgaggcgatg
    13981 gacgccttct cgctgttgtg caagaccgaa ggcatcatcc ccgcgatcga gtcggcgcac
    14041 gcgatcgcgg gcgcgttgaa gctaggtgtt gagttgggtc gcgggtcgat catcctggtg
    14101 aacatgtcgg gccgcggcga caaggatgtc gagacggccg ccaagtggtt tggcctgttg
    14161 gatggtgcgt catgagccgg cttgcgtcgt tgtttgaaga ctgcggcgcg gagggccggt
    14221 cggcgttgat cggctacttg ccaaccgggt ttccggatgt gccgacgtcg atttcggcga
    14281 tgacggcgct ggtcgaatcc ggttgcgaca tcatcgaagt cggtattgcc tactccgatc
    14341 cggggatgga cggccccgtc atcgcggcgg cgacggaggc agcgttgcgc ggcggcgtgc
    14401 gggtgcgcga cgcgctggcg gcggtcgagg cgatcagcaa ggccggcgga cgggccgtcg
    14461 tgatgactta ctggaatccg gtgttgcgtt ggggcgtcga tgcgttcgcg cgtgacttgg
    14521 catctgcggg tgggctcggg atgatcacgc ctgacttgat tccggacgag gccgatgaat
    14581 ggttggccgc gtcggagacc cacaatctgg accggatctt cttggtggca ccgtcgtcga
    14641 cgccggagcg gctggcggcg acggtcaagg cgtcacgcgg attcgtttac gccgcttcga
    14701 caatgggggt gacgggcgcg cgtgacgccg tgtcggtggc ggcgccggaa ctggtgcgcc
    14761 gggtgaaggc ggtgtccgac attccggttg gtgtcggctt gggcgtgcga tcccgcgagc
    14821 aggcggcgga gatcggcgcc tatgcggacg gggtcatcgt ggggtcggcg ttggtgtcgg
    14881 cgttgaagga cggaattccg gcggtgcggt cgttgaccga ggaactcgcc ggcggtgtgc
    14941 ggcagaggat tactgcgtga cgacgactta cctggcctat ataccgagcc cgccgcaggg
    15001 cgtttggcac ctcagtttta taccgatccg ggcgtatgcc ttgttcatcg tcgcgggcat
    15061 cgtggtggcg ctgatgctcg gcgaccggcg ctgggttgcg cgtggcggcc aagccggggt
    15121 catctacgac attgcgttat tggccgtccc gttcggtctg atcggtggac ggctctatca
    15181 cgtcatcacg gactggcgga cgtacttcgg tgacgatgga gcgggactgc tgggcgcatt
    15241 ccggatctgg gatggcggcc tcgggatctg gggcgctgtc gcgctcggcg gtgtcggtgc
    15301 gtggatcgga tgccgctata agggcattcc gctgcccgcg ttcggcgacg cgatcgcgcc
    15361 gggaatcatt ctggcccaag ccatcggccg ggtgggcaac tatttcaatc aggagctgta
    15421 cgggcgaccg acaacgttgc cctgggggct cgagatctat gagcgcgtca atgccaatgg
    15481 tgcgccggac aatttgaatg gtgtgtcgac gggcgagttg ctgaacattt accacccgac
    15541 gttcttgtac gaactgctgt ggaatgtctt gattttcgcg gcgctgatct atatcgaccg
    15601 gcgattcaag atcggccacg gtcgattgtt cgcgatgtat gtggccggct attgctttgg
    15661 gcggttctgg atcgagttga tgcgaagtga ctatgcctca cagccattcg acggaattcg
    15721 ggtcaactcg ttgacgtcga cgttcgtatt cattggcgcc gtggtgtaca tcatggtcgc
    15781 accgaagggc cgagaagatc ccgcctcgct tgccggcaag ccggaggaag acgaagacac
    15841 ggtcgagacg ttcgccaaag agttgacggc ggtggccgcc acgactgggg tcgtcgccgc
    15901 tgccaaggcc gccggtgacg cggaagatga agacgccaaa gacgcagttg aggacgagaa
    15961 gcccgaggag cccgacgaac cagcggttaa agacgcggaa gcattcatga cggacgtgcc
    16021 ggccgaatcc gttgaggaag ctgaggaatt cgccgccgaa ggtgaggcag aacctgaagc
    16081 aggattagga gaagtagagg gcgaagaagc ggtcgaggca ctggcggctg cgccggaggc
    16141 tcatgaggcg gcggtggaag ccgccgaaga ggccgaggcc gtcgccgacg aggtggccgc
    16201 ggaagatgag gccgaagagc cagaggcaga agccgaagag ccagaagcag agaccgagga
    16261 cacaaccgac gtacagccgg aagcggaatc ggccgaacag ccagaggccg aaaccgccga
    16321 gcctgaggca ggcttgggtg cggtcgaagg cgaagaagcg gtcgaggcac tcgaggccgc
    16381 cccagatgtt catgacgcgg cggtagaggc cgctgacgag gccgaggctg tcgccgatga
    16441 ggtggctgcc gaggaagagc cgtcatcgga agcagcggct gaagcaggag cacttgccga
    16501 ggatgtcgcc gaagcgcctt cggaggacat tgcggaggcc gaggaattcg cggaggcggg
    16561 tgaagaggcc gagcaagcgg aagccgctgc ggagtcagaa gaatccgagc ccgacgagcc
    16621 ggaaaccgag gacgccgaag cagcggtcga agaggcggaa gcagaaccgt ccgtagaaga
    16681 ggccgccgag gaagccgaag cggtggtcga ggaagccgat tccgctgcgg aagacgaacc
    16741 gaccactggg gttttggagg ccaagacaga accggagcag acgcccgaac cggccgcgcc
    16801 cactgaggaa accggtggtc gtctacgccg ctggctccgc cgcgaccgct aatcggagcc
    16861 atccccaaac tcgaattctc cacatgctgg cctgaagtgt ctgtgtcgat tccaccgagt
    16921 gcacaagcgc ttacggcaag cgaaatcagt ttcgccgcaa cccactgcta cggcgaggcg
    16981 acggcgaata agtgccagtc gccgggcacg cccttgagtt cgtgagtgcc tcgctcttcg
    17041 aattcgagtc ctgacccgat caccaggtcg cgcagcgtgc tggacacgag cacctcgttc
    17101 ggccctgcca ttgcgctcac gcgcgcgccg atgtgcacgc cgataccacc gatgtcgtcg
    17161 ccgcggacct cgcactcgcc ggtgtgcaac cccgcacgca cctcaatgcc gatggattgc
    17221 accgcctcgc gaatcgccat ggcgcagcgg atcgctcgct gagggccgtc gaacgtcgcg
    17281 aggaagccgt cgcccaacgt gttcacctcc cggcctcgaa accgagacag ctgcgcacgg
    17341 acgacggcgt cgtgcgcatc aagcaacgca tgccagtcac ggtcaccgat ctccgccgcc
    17401 ttgcgtgtcg agtccacgat gtcggtgaac agcaccgtgg cgagcacccg atcatcggcc
    17461 acttcagcct ggtggccggt gaggaacttg gcgatctcct gaaaagacgc gcgccacggt
    17521 tccacgatgt gccaggcgtt gcgccctggc agctcgacgt atttcgcgtc cggtatgtgg
    17581 tcggcgatgt acttgcccga tgcacattgg aaaaatcggt cgtcggcgtg ctggacgaca
    17641 agggtcggca cacgaatcgt tggcagcacc gcccgcacgt ccacttcggc caccagcggc
    17701 agcatgagag caacggttcc tgggctcgcc ccaaggcgct ccaatcgagc ccatgtcgcc
    17761 cggatctcct cgttccacgg catgtccgga ttgaccacat gttggaatgc gcccgacccc
    17821 cacatggcga ctatggcagc gttggctccc tcgcgatcga gtccatcggt gctttgagcc
    17881 accggatcat ccgcgtagcc ctcgagcaca accagcgcgg tggtgcggga tggatgtgtg
    17941 gccgcgaaca gcgcccctgt cgcgatggcg ccgccgaccg cgaggaggac cgcttcgcga
    18001 ctcccgaggt cgtcgagcac cgcggtgatg ctgtcggccc attgctccaa ggtcggcagc
    18061 gcgcccggcg tgatgggatc ggacgctccc gtgcccggct ggtcgaagag gatcagtcga
    18121 ccgagcgaca tcatcgcctc gacccaccct tgaacggacg gtagctccgg agcgacctca
    18181 cagtttgtga accagttaga gacgaacacg aggtcgcgag ggccttcacg cgacgcgcga
    18241 taggcgacgc gcaagtcccc attcagcgca tatcgcgtct ccgagaacac ggcggcagtg
    18301 taagccgagg agcgggtcca agttccggtg tcagacaagc gcgccagtat cagccgccct
    18361 tcgcgcctat ccgcttgcag gcacatgtag gcgacgaccg accaggcgcc gacacacagc
    18421 acgaaccaca gcggcggtgg caccgacgcc gtcgcctcgg ccagacgcgc gcggcgacct
    18481 tcgcgacgct gcgcctgctg gtcgaaccac tgcgcgtagg ccgcctcctg cttggcgctc
    18541 gtcggctcaa tgccctcgaa gtcccgaccc atcgtctcga cccaggcaag taccacctcc
    18601 gaggacttcc ctgctctcat cgcaggccac tcatcgaaga tgacggcgcg cgaatagcag
    18661 ataagtcccc cgtgcagagc ccggcccgaa tgcccctcca acacgtcggc gacatcgtga
    18721 agctcggtga ccgccacgcc ttcgacgctc gcgccgtcgc gggcgtgttg gtaactctgc
    18781 agcgagaaga agatgacgaa cgcgagcaac accgcggtca tcgtgccgac cacggacaaa
    18841 gtggtggccg cgcgcggcgg atcggcgaac cagccctgct caggtatccg acttcgcacg
    18901 aagagcctgc tgactatcgc cgcggacacg atcacgatga ggatcagaag cgcgatccag
    18961 gtcagcaccg ccgtactgta gctgtgcgcg acgtcgaccg gcgtgggttt gtaacgttcg
    19021 acggtgacgc gtgcagaagg ccggacggtg gtcctccttc acaggctcgg cggtgaccgc
    19081 ctgatgcgca acagttcgcc gtgtggggcc aagcgcgtgc agacgaccac gggatggtcg
    19141 tgggccgtgc tggcgctggc ggggcaatcc agatatgcca cagcgtgact tgcagggtca
    19201 cccggtcgaa attccacgta ttctccacac ctctgagctg cggttatatc cgcacgcgat
    19261 actgggattg aagtaggagt aaaagtgttc ttcgcgtatt cggtgacagc acgtgctgca
    19321 gacgttcaag agcggtcgct acaccgccag tagagcttct accgccgacg gcttcttgta
    19381 aaacacgtcg agttccacga tcaacccgtc gcgcaccgtg aacacctcag ccatgctcat
    19441 ctcgacactc tcgccggacg cgcgtgccgt gaacctcagg cgatagtgga gcacgacttt
    19501 gtcgccgtcg gcgagaaact gcatctgtgg gctcggcgtg agctcaagcg catcggagat
    19561 cttggtgatt agctcgagaa agcctgctgg gccacagtaa tcgccgctat atggcacgtc
    19621 tccggctgca tgaacaacaa actcgtcgtg caacaggctc agtgcgtcgt cgagcctctg
    19681 atcgccgaac gcgtcgacga acttcacgac gatcgcgcgg caatcctcgg tctgggtcgt
    19741 catcggacct cccaccctcg cggatatcgc gggcgaagtc tacgcagagc cgaacaggtc
    19801 atccgacgat tcggttcagc tggtacggtt gaagaccttt gatacggcgt gggtggctgg
    19861 ccgtcgcaag atcaacggtt accaaactgt gggcgcctga aggtttgggt ttattgcgcc
    19921 gaaagtggct gttccggtat cgcattagcc accgcagatg cctgctccgt agctgccgca
    19981 gatgcctgac ccggcaccgc tttagctgcc gcaagcgcca actgcctgcg cttaacccac
    20041 agccaaaccg ctatacctat cgcaccgaca gcaaaaagca ccgcacccac cacgatggac
    20101 caactccgac tcatgctctg cctcgcaatg ttgggttgtg gttcgcaaac gccctgaccg
    20161 ctgatactgg ccggccaagt gggcttttga ccccccggtc aatttggggt acccgcctct
    20221 ctgacggctg aaaacatctt tcgagtggcc gacccatata aaggacatca acacagagcg
    20281 tgcgtgctgt gcggccggtg tggtgctggc gttttcgaca gacatgacgc cgccgcccag
    20341 ctgcttaacg aaaaatcaca gcaaacatat cgccgccatc ggacctcgtc cttgtcgtag
    20401 ccgcgtccga ggcgcggctc agagaaagcc atgttgcgga cctgctctgg agtaagccaa
    20461 ccgcgcgttg tcttgtgtcg ctggtttgcg cgggggtcgc ccggcggcgc gcggctcggc
    20521 tagccgacgt tccagctccg cgatgcgctt ctccacacgg gtggaaattg tggacacccc
    20581 atccaggtag ccggctgcat ttcccgacgc ggtgcaagcc aactgcgccc ttcagcgcat
    20641 cggcctgtac gcccaacgcc taaccgaacc gtggcctaaa gatgcggcgc cgcaagcaca
    20701 ctcgcgaata caaccggctc taggtggcag ggacggcccg cgtgacgtgg tagtggcgat
    20761 cgtcgggctg agcgagcata gcggtcatgg tctttcggtc gtagggccac agatcgacgt
    20821 tgccttcgtc ggtcaggtac caggagttgc aaccggtgtt ccagaccgta gggccgagtg
    20881 ctgcggcgac gtcgtcgttg aacttcgtcg tggcttcctc ggtgacctcc acggtgtcga
    20941 tctcaccgtc acgaaatcgc cgcagccact gggtgataaa gcgggcggtc aattccgcgg
    21001 agtactgcag cgagatggag cccgtcggcg agttgggccc caacaccgtg aagaaattgg
    21061 ggaaccccgg aatggcggac atgcggtatg cgcgtggccc cttggcccag gcgtcgtcga
    21121 tcgacacacc atcgcggccg cgtagattca tcggtcgcat gtagttgtgc gcctgaaatc
    21181 ccgtggcgaa caccaacaca tcgacatcga tctcgcgacc gtcggctgtg cggatgccgc
    21241 gcggcgtgat tcgttcgatc gcgtcggtca ccagctcggc attgcgtgct cgaagcgccc
    21301 ggtagtaaga ggccgagaca acctgacgct tgcacaacgg ctgatagtca ggagttagcc
    21361 gactgcgcaa ctcgcgatcc cggacctggg tacgcaaaga ccaccgcgcg aagtcctgca
    21421 ccagccttcg acgccaactc ggccgggtcg tcacgtcggc gaggatgccc gacgcccaca
    21481 gcagcactcc atacatccac cggtgcacac ccggcagcct tcgtagtgcc gcaccgagaa
    21541 ttgccggttg gggcagtccc atcggcgccc acatcaccca ttgcggtgtc cgcacgaagt
    21601 gggtgatgtg tttcgccccg cgttggagcg ccgaaacgac ctgcacaccc gttgaacccg
    21661 tgccgatgac ggcgatcttc cggccactgg tcacgacgtc gtcatcccag cgcgcggtgt
    21721 gcaccacatc gccacggaac gtgtccagcc cgggaatgtc cggagtaaac ggatgatgca
    21781 gcacaccggt cgcggcgatc acgaagtccg cctttagctc gccgccgtcg gaagtcctta
    21841 tctgccagcc ggatccggtg aacaccgcgg agacgacctc cgcgttcagc cgaaggtggt
    21901 catccaggcc gaaacgttct acgacgtcgc gcaggtatcg ctgtatttcc tcacccgatg
    21961 cgaacaacga tgtccagtcc ggtttcggtg cgaacgagaa ctggtagagc tgcgacggca
    22021 catcgcatgt caggccgggg taccggttcc aatgccagac gccaccgacg tcggcgccct
    22081 tttccaggat ggtgaagtcg tgaaatccct cccgctgcag ggtcactgcg gtcgcgatgc
    22141 ccgcaatccc cgcaccgatg atgacgatcc tcggctggcg acccgtcatg actgtgcctc
    22201 cggtgcttgc ccccggatcg tctgctcgta ctcctcgcgc acgtcgtgtt cgatcgaggt
    22261 caatgcccgt cggtcaccga tcagcttgcg gcccaagcgc attaggcgtt ccgggaccgc
    22321 tgcgttgacc agatcccaac ccgccaggta ccgcgggacg gtgatctcgg cccgccggct
    22381 gtcgacactg ccgacgatcg cccgtgcgac gtcttccgga tcgacggtcg gcatgccatg
    22441 gccgagcggc acgccggacg ccagccgggt acgcactgcg ctcggcaaca ccgcggacac
    22501 gctcacccct gtatcccggt actcctcccg caccgccgcg gataggccca ccgcggcgaa
    22561 cttactcgcg ttgtagaccg ccatgcccgg aacgaccagc ttgccggcca tggatgcgac
    22621 gttgacgacg tgaccgctgc cgcgttcgat catgtgcggc atgaccagcc gcatgccgtg
    22681 aatgagcccc cacacgttga cgtcgaccgt cgtcctgctc gtggcgtcgt cctcggacag
    22741 aaagtctccc aacggcatca cgccggcgtt gttcaccagg acgtcgatgc ggccgaaccg
    22801 ggccagcact gcggcggtga attctgcgaa agactcgcgc gacgtcacgt ccagcgggta
    22861 attgccgcca tccagatcac cgacacctac cgtcgcgccc ttggccgcga agagctccgc
    22921 cgtggccctt ccgatgccgc gagcaccacc ggtgacagcg acgacggcgc cgtcgagctc
    22981 gatcttgggg taggacatca cggcctccta tcgatttcga atactgacgg tatctggaga
    23041 ctataggtat gtcaatgggc tagaatcgca gcatggccag gaagtcccga gcagaaacac
    23101 aggccgagac gagggctcag ctggtcaaga ccgccaggca gttgttcttc gaggacggct
    23161 accacccgac gtcactcgag aaggtcgccg acgcggccgg ctactccaaa ggcgcggtct
    23221 actcgaactt ccgcaacaag gacgaactgt gcaccgcggt gctcgacgag gtccgggccg
    23281 agcgcctcgg ggaggtcgtc gagatcttcg gtaagccgga cacgccctcc cgcatcgacg
    23341 cgatacggga ctgggccgaa cgcgtgatcg gcgatccagg ctggacgacg ttggaggtcg
    23401 agttcgccgc gcacgcgcgg cccaacgagc aactgcgaac cgaattggca ggtcgcctgg
    23461 atggcatcct ccaaatgctc accgccgcaa cagaatccgc cgatactgcc gacctcaaga
    23521 tgccggctcg cgaagcctcg acaatcctgc tcgccctcgg cgtcggactc gggctgctcc
    23581 gctcgatcga tcccgcgata cccgtcagcg ggctcgtcga cacactgcgc ctgctatccg
    23641 gacagtccct gtaacccgca atatttggtg gctttcctta cttacgacag ggcggatggc
    23701 tctggcatgc tgggtgccat gaccgagccg cagttcagtg gcagcgaaag cggaaacccc
    23761 tacccgccgc ccccgcagca gccgggttat ccgccgccgg ctggccaata tccgccgccg
    23821 ccagggcagt acccgccgcc cccagggccc tatccgccgg cgtacgtgga tccggccgca
    23881 ccctacggcc gtcatccgct gaccggcgag ccgctgtcgg acaaatccaa gttggttgcc
    23941 ggcctgctgc agctgattgg attgttcggt ctggtcggca tcggccgcat ttacctcggc
    24001 tacaccagct tgggcgtcgt gcagcttgtc gtggggctga tcacctgcgg catcggcgcg
    24061 gtcgtctggg gcatcgtcga cgcggttctg ctcttcacgg acaaggtccg cgaccccgag
    24121 gggcgtccac tgcgtgatgg cacctagcgc caccactcgg cgatccctct acggtgcact
    24181 cggcaccggg gccgtactcg tcggtgggct ggcctacgtc gggctcggcg acccgcacag
    24241 cccgggcttc gtcttccctg tctgcccatt caaggcgctg acgggctgga actgccccgc
    24301 ctgtggcggc ttgaggatga cccacgacgt gctgcacggt gacctcggcg ccgccgtcgt
    24361 cgacaacgtc tttcttcttg tgggcctgcc cctgctggcg gcctggcttc tggtgcgctg
    24421 gcgccgcggg cagtcgttga tgccgatgcc ggcgatcgtc gtcatcgcca tggccgccat
    24481 cacttggacg gtggtacgaa acctgcccgg gttcccgctg gtcccgacgc ttcttaccgg
    24541 gtagttccgc agctgcgctg atacaatcga caaccgggcc ggtgcagtcc cggaccaaga
    24601 tttttccgga ctgcggaggt accgccaatg ctgttttcgg cattgcccga ccgccagggc
    24661 ttgtacgacc cagagaacga gtcggattcc tgtggcgtcg ccatggtcac cgacatccag
    24721 ggtcgacgct cgcacgccat cgtcgccgac gggcttattg cgctggagca cctcgaacac
    24781 cgcggtgccg cgggcgcgga gcccaacagc ggtgacggtg ccggcatcct gatccagctg
    24841 cccgtcgaac ttctcaagga ggtcgtcgac ttcgagcttc cggcgcccgc cgacgacggg
    24901 tgcaacacct tcgccgccgg tatctgcttc atgccgcaag acccggacga ccgcgcgaag
    24961 gctcgcgaac atgtcgaggc catcgcgaaa gaggaggggc tgcacgtcct cggttggcgt
    25021 gaggtgcccg tcgacccaca cggcgccgaa gtcggcgtca cggcgctgag ttgcatgccc
    25081 tacatgacgc agctgttcgt ctccgcaccc ggacaaagcg gcatcgacct cgaccgccgg
    25141 atctacccat tgagaaagcg tgccgaacgc aacggcgtgt atttcccgtc gctttccagc
    25201 cgcacgatgg tttacaaggg catgctcacg acaatgcagc tgccccaata ctttccggac
    25261 ctgcgcgacg aacgctgtat gagtgcaatc gcgatcgtgc acagccggtt ctccaccaac
    25321 accttcccgt cctggccgct ggcccacccg ttccgcttcg tcgcgcacaa cggcgaaatc
    25381 aacactgtgc gcggcaaccg caaccgtatg cacgcccgcg aggccatgct cgccagcgcg
    25441 aagattcccg gcgacctggc ccggctctcg ccgatctgca ccccggaggc ctccgactcg
    25501 gcgtccttcg acgaggtgct cgaactgctg cacctcggcg ggcgcagcct gccgcacgcc
    25561 gtgctgatga tgattcccga ggcgtgggag aacaacagca ccatggaccc cgccgagcgg
    25621 gcgttctggc agttccacgc ctccctgatg gagccgtggg acggtccggc gtgcgtgacg
    25681 ttcaccgatg gcacgctcgt gggagcggtg ttggaccgca acggattacg gccaggacgc
    25741 tggtggcgca ccatcgacga ccgcgtgatc ctggccagcg aaagcggtgt gctcgacgtg
    25801 ccgtctgccg agatcgttgc caagggccgc cttcagcccg gcaagatgtt cctgatcgac
    25861 accgcgcagg gccgcatcgt cgccgacgac gaaatcaaag acaacctgag caagcaagaa
    25921 ccctacggcg aatggctgca cgccggcctg ctcgatctca agacgcttcc ggaccgcgtg
    25981 cgcgtccagc ccaaccacga atccgttgtg cgacggcaga tctccttcgg ctacaccgag
    26041 gaagaccttc gcgtcctgct cactccgatg gccgcatcgg gtgccgaacc actcggctcg
    26101 atgggtaccg acaccccgac cgccgtgctg tctcagcgct cccggttgct ctacgactac
    26161 ttcgtcgaac tcttcgctca ggtgaccaac ccaccactgg acgccatccg cgaagaggtc
    26221 gtcacctcga tggcccgggt gatgggtccc gagcagaacc tgctggcgcc gaccgccgcg
    26281 tcctgccgcc aaatcgtgct gagatggccg gttctcgaca acgatgagct caacaagatc
    26341 gtccacatca acgacgaccg tgagcacccc gggctgaaga ccgctgtgct gaaggcactc
    26401 cacgatgtcg aacgcggtgg tgaaggccta gccgacgcgc tcgacgatct gcagcagcgg
    26461 gcctgtgacg cgatcgccaa aggcgcacgt acactggtca tctcggaccg ggactccgac
    26521 cacaccaggg cgccgatccc gtcgctactg gccatttcgg ccgtgcacca ccatctcgtc
    26581 cgcaccaagc aacgcaccac cgttgcactc gtcgtggaaa gcggtgacgc ccgcgaggtg
    26641 caccacatcg cgatgctgat cggcttcggc gcggcggccg tcaacccgta tctggccttc
    26701 gagtccatcg acgacctcat ccgggaaggt gagctcacgg gcatcgaacc cgccgccgcg
    26761 gtgcgcaatt acgtcaaggc gctcggcaag ggcgtggtga aggtgatgag caagatgggc
    26821 atctccaccg tcgcctccta caccgccgcg caggcgttcg aggcggtcgg catcaacaag
    26881 gacgtcatcg acgagtactt caccggcacg ccgtgccaac tcggcggtgc cggactggac
    26941 gtgatcgccg aagaggtcaa acagcgacac cgccgcgcgt atccggagaa ccccaccgag
    27001 cgggtgcatc gccgcctcga ggtgggcggc gaatacgcct tccgccgcga aggcgagctg
    27061 cacctgttca ccccggaagt ggtgttcctg ctgcagcatt cgacccgtac cggtcggcac
    27121 gacatcttcg gtcagtactc cgacgaggtt aaccgcctct cgaaggaagg cggcaccctg
    27181 cgggggctgt tcgacttcaa gaagttaagg ccgcccgtgc cgctcgagga cgtcgagcct
    27241 gttgattcga ttgtcacccg cttcaacacc ggcgcaatga gttacggctc gatctccgcc
    27301 gcgg
//
LOCUS       DASRJA010000236         3748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3594447,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000236 DASRJA010000000
VERSION     DASRJA010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3594447"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..561)
                     /locus_tag="VFC01_10845"
     CDS             complement(1..561)
                     /locus_tag="VFC01_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6611 family protein"
                     /protein_id="HZN80169.1"
                     /translation="MNDIRTGSGLLRRGWSRLLDGDRAWGSLEIRPDRFGVTRYRLVV
                     FPPGISDTERRQVRVARGWPLWGALVWVFCEIWLSHMTSPWTALVASTVIYFGLGLVA
                     TARAGDPRRQVRTIGAMVMAGHHDPASAAVRHRLENLAGTLMEADERLARGEISATTH
                     EMTWWRVYDQMAPSASPGMHPSDTGA"
     gene            677..1711
                     /locus_tag="VFC01_10850"
     CDS             677..1711
                     /locus_tag="VFC01_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZN80170.1"
                     /translation="MTIENLPTRLADHPTVRKVRSRTTEKPGVIDADWLRQVCRDAGV
                     DDVAFASVENPKLASEREHVDAALPGTQSYISLVVKMNRDNVRSTARSVANQEFHRSG
                     EIMNEAAHRITRALEDAGYRAINPSMSFPMEMDRFPGRIWVVAHKPVAVAAGLGVMGI
                     HRNVIHPKFGNFILLGTVLVAAPISSYGEPLDYSPCLECKLCVAACPVGAIKKDGDFD
                     FVACSVHNYREFMGGFTDWAQTIADSADAEDFRSRVSDSENASMWQSLSFKANYKAAY
                     CLAVCPAGEDVIEPYLDDRKGFMDLVLKPLQEKKETLYVLPNSAAKAHAERRYPHKQV
                     KVVDSGIRGR"
     gene            1757..2518
                     /locus_tag="VFC01_10855"
     CDS             1757..2518
                     /locus_tag="VFC01_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN80171.1"
                     /translation="MKITEVAAFRAKSGTVPPLRRLMMCVAAVLFSGVTLVAPAVVAP
                     GATAVASAQADACPAVQVVFARGTGEPDGTGRVGQAFVDALRPMVKGKTVNVYAVNYP
                     ATRDFLRAADGANDASLFVQNIATTCPDTKIVLGGYSQGAAVIDAITVADQPTLGFTQ
                     PMPADVANHVAAVAVFGNPSIRLLGNPLTALSPLYGNKTIDLCNGADPVCSDGNDVPA
                     HSLYEQSGLTTQAAQFVADRIANPQAPISTLAGQG"
     gene            complement(2515..3093)
                     /gene="pth"
                     /locus_tag="VFC01_10860"
     CDS             complement(2515..3093)
                     /gene="pth"
                     /locus_tag="VFC01_10860"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZN80172.1"
                     /translation="MAEPVLVVGLGNPGPQYATTRHNLGFLVADILSDHLGSGFKVHK
                     KSGAEVATGRLGGRSVVLAKPRCYMNESGRQVGPLAKFYSVPPADIVVIHDELDIDFG
                     RIRLKFGGGVAGHNGLRSVGAALGTNDFQRVRIGIGRPPGRMDGASFVLSNFTNTEWR
                     EVPTICEQAADATELLVAQGLEPAQNTVHAWG"
     gene            complement(3106..>3748)
                     /locus_tag="VFC01_10865"
     CDS             complement(3106..>3748)
                     /locus_tag="VFC01_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HZN80173.1"
                     /translation="AGVRTETGKGASRRARRDGKVPAVLYGHGSDPQHLELDAHDFSA
                     VLRHSGTNAVLTLDIEGKEQLALTKALEIHPIRRNIQHADLLVVRRGEKVTVEVNVVV
                     EGEAAYGTLVTQDANTITIEADVQSIPDHLTVSVEGAEIGTQFAAASITLPSGVTLVS
                     DPEMLVVNVVAAPTAEDLEAEGAGEVAEAAEVEEAAEGAEGEGAEEAPAEESE"
BASE COUNT          631 a         1276 c         1215 g          626 t
ORIGIN      
        1 ctaggcgccc gtgtctgacg ggtgcatgcc gggcgatgcg ctcggagcca tctggtcgta
       61 cacccgccac caagtcatct cgtgggtcgt tgccgagatc tcgccccgcg cgaggcgttc
      121 gtcggcctcc atcagcgtgc ccgcaagatt ctccaacctg tgacggacag ccgctgaggc
      181 cggatcgtgg tggcctgcca tcaccatcgc gccgatcgtt ctgacttgcc tgcgcggatc
      241 gcccgccctc gccgtcgcga cgaggcccag tccgaaatag atcacggtcg acgcgaccag
      301 agcagtccat ggactggtca tgtggctcag ccagatctcg cagaacaccc acaccagcgc
      361 accccacagc ggccaacccc gggccacccg aacctggcgc cgctcggtgt cgctgatgcc
      421 cggcgggaac accaccaggc ggtatcgagt caccccgaaa cggtccggtc ggatttcgag
      481 cgacccccac gcacggtcgc cgtcgagcaa ccgtgaccac ccccggcgta gcagaccgga
      541 gcccgtacgt atgtcgttca tgtctccggt gtacccgcgt cagcgcaatg aatgtgcggt
      601 ccgaacactt tctttacgac gtgatgcggc tcccttaacg gcggcgacca gctgagtagt
      661 gtgtactata ctcagcatga cgatcgagaa cctcccgacc cggctggccg accatccgac
      721 ggttcgcaag gtgcggtccc ggacgacgga gaagcccggc gtcatcgacg cggactggct
      781 gcgccaagtg tgtcgtgatg cgggtgtcga cgacgttgcc ttcgccagcg tcgagaaccc
      841 gaagctggcc agtgagcgcg agcacgtcga cgcggcgctg cccgggacgc agagctacat
      901 ctcgctggtc gtcaagatga atcgcgacaa cgtgcgctcg accgcgcgca gcgtggccaa
      961 tcaggagttc caccgcagcg gcgagatcat gaacgaggcg gcgcaccgga tcacccgcgc
     1021 gctagaggac gcggggtacc gcgcgatcaa cccgtcgatg tccttcccga tggagatgga
     1081 caggttcccc ggccgcatct gggtggtggc gcacaagccg gtcgcggtgg ccgcaggcct
     1141 tggcgtgatg ggcatccatc gcaatgtgat ccacccgaag ttcggcaact tcatcctgct
     1201 cggcacggtc ctggtcgccg caccgatcag cagttacggt gagccgcttg actattcacc
     1261 gtgcctggag tgcaagttgt gcgtcgcggc ctgcccggtc ggggcgatca agaaggacgg
     1321 cgacttcgat ttcgtcgcct gctcggtgca caactaccgc gagttcatgg gtggcttcac
     1381 cgactgggcg cagacgatcg ccgacagcgc cgacgccgag gacttccgat cgcgtgtcag
     1441 cgattccgag aacgcgtcga tgtggcagag cttgtcgttc aaggccaact acaaggcggc
     1501 ctactgcctc gcagtgtgcc ccgccggcga ggacgtcatc gagccctatc tcgacgaccg
     1561 caagggcttc atggatctgg tactcaagcc gctgcaggag aagaaggaaa cgctgtacgt
     1621 gctgccgaat tcggcggcca aggcgcacgc cgaacgacgt tacccgcaca agcaagtgaa
     1681 ggtcgtcgac agcgggatcc gaggccgcta gccgggctaa ggaataatcc tgcgacccgc
     1741 ggacgtttgc caacgcatga agatcaccga ggtggcggcg tttcgcgcca agtcaggaac
     1801 cgttccgccg ctgcgtcggc tgatgatgtg cgttgcggca gtgttgtttt cgggtgtgac
     1861 gttggtcgca cctgcggtag tcgccccggg cgcgacggcc gtcgcctcgg cgcaggccga
     1921 tgcgtgtccg gccgttcagg tcgttttcgc gcgcggcacg ggcgagccgg acggcaccgg
     1981 gcgggtcggc caggcgttcg tcgacgcact gcgccccatg gtcaaaggca agaccgtcaa
     2041 cgtctacgcc gtcaactacc ccgcaacgcg ggacttcctg cgcgccgccg acggcgccaa
     2101 cgacgccagc ctgttcgtgc agaacatcgc taccacctgc ccggacacca agatcgtcct
     2161 cggcgggtac tcgcagggcg cggccgtcat cgacgccatc acggtggccg atcagccgac
     2221 cctcggcttc acccagccga tgcctgccga cgtggccaac cacgttgccg cggtggccgt
     2281 gttcggtaac ccgtcaatcc gcctgctcgg caacccgttg accgcattga gcccgctgta
     2341 cggcaacaag acgatcgacc tgtgcaatgg cgcagatccg gtctgctcgg acggcaacga
     2401 cgtccccgcg cacagcctct acgagcagtc cggattgaca actcaggcag cgcaattcgt
     2461 cgctgaccgg atcgccaacc cccaggcgcc gattagcacg ctggccggcc agggctaacc
     2521 ccaggcgtgc acagtgttct gtgctggctc caagccttgg gccacaagta gttcggtggc
     2581 gtcggccgcc tgctcgcaga tcgtggggac ctcccgccat tcggtgttgg tgaaattact
     2641 caacacgaat gacgcgccgt ccatgcggcc gggcgggcgg ccgatgccga tgcgcacccg
     2701 ctgaaagtcg ttggtaccta aggctgctcc cactgagcgc agcccgttgt gccctgcgac
     2761 gccgccgccg aacttcagcc ggatgcggcc aaagtcgatg tccagttcgt cgtggatcac
     2821 cacgatgtcg gcgggaggca ccgagtagaa cttggccaac ggcccgacct ggcgcccgga
     2881 ctcgttcatg tagcaccgcg gtttggccag cacgaccgac cggccgccga ggcggcccgt
     2941 cgccacctcg gcccccgact tcttgtgcac cttgaacccc gagccgagat ggtccgaaag
     3001 gatgtcggcc acaaggaaac cgaggttgtg tcgggtcgtc gcgtattgcg gtccagggtt
     3061 gcccaggccg accaccaaaa caggttcggc catctcggtg tacgcctact cggactcttc
     3121 ggcgggcgcc tcttcggcgc cctcgccctc ggcgccctcg gcagcttcct caacctcggc
     3181 agcttcggcc acctcgccgg cgccctcggc ctcgaggtcc tctgccgtcg gcgccgcgac
     3241 gacgttgacg acgagcatct ctgggtccga caccagggtg actcccgagg gcagcgtgat
     3301 cgaggccgcg gcgaactgcg tgccgatctc ggccccctcg accgaaaccg tgaggtggtc
     3361 gggaatcgac tgcacgtcgg cttcgatcgt gatggtgttg gcgtcctgag tgaccaaggt
     3421 gccgtaggcg gcctcaccct cgacgacgac gttgacctcg acggtcacct tctcgccgcg
     3481 gcgcacgacg agcaggtcgg cgtgctggat gttgcggcgg atcgggtgga tctcgagcgc
     3541 cttggtcagc gcgagctgct ccttgccctc gatgtcgagg gtgagcaccg cgttggtgcc
     3601 agagtgccgg agcaccgccg agaagtcatg ggcgtcgagt tcgaggtgct gcggatcgct
     3661 gccgtggccg tagagaacgg cgggcacctt gccgtcgcgg cgggcgcgac gcgacgcgcc
     3721 cttgcccgtc tcggtgcgga cgccagcg
//
LOCUS       DASRJA010000237        18598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1359676,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000237 DASRJA010000000
VERSION     DASRJA010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18598
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359676"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..370
                     /locus_tag="VFC01_10870"
                     /pseudo
     CDS             1..370
                     /locus_tag="VFC01_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730131.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(371..1201)
                     /locus_tag="VFC01_10875"
     CDS             complement(371..1201)
                     /locus_tag="VFC01_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80174.1"
                     /translation="MESYDGRATVITGGANGIGFATAREFARRGARVMLADIDQSALD
                     DAVATLRADGIDAHGVLCDVRKLDDVNHLADEAFRVFGDVHVVFNNAGIAYAGPIAET
                     SHDDWRFVIDVDLWGPIHGVEAFLPRLGSGSHMVFTSSFAGLIPNVGLGPYCVAKYGV
                     VALAETLAREARPNGIGVTVLCPMIVETNLLANTERVRSADYGPAHSDAETVQELASA
                     PDDDSVLNVEDVARLTADAILANRLYVLPHRAARDSIRRRFERIDRTFDEQAAEGWSH
                     "
     gene            complement(1220..2296)
                     /locus_tag="VFC01_10880"
     CDS             complement(1220..2296)
                     /locus_tag="VFC01_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80175.1"
                     /translation="MADTVLVTGGFGLVGSATVGKLAADGRRVVVADLESPANVKKIK
                     ALPGGVEVRWTDLTDPDQVQRVISDVAPAAIIHLAAIIAPAIYRVPQIARRVNVDATE
                     TLVRIAEAQPNPPRFVHASSNAVFGPRNPHRTPEPLRADDPMRPCDIYSGTKAEAEEI
                     VQSSTLEWVVLRFGGVLSIDLSALPLSTDAMFFESALPSDNRVQTVDVRDVAWACAAA
                     TTADVAREILLIAGDESHRRRYGDLTPAVASALGMPGAVPPGRPGNPDSDDDWFVTDW
                     MDTTRAQEALKFQHYSWPDMMTELRAQFGWKRYPARLIVPIAREVMKRRGAYWKVPGK
                     YADPWGAICTKLGDPSWDRPYDRG"
     gene            2387..3190
                     /locus_tag="VFC01_10885"
     CDS             2387..3190
                     /locus_tag="VFC01_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN80176.1"
                     /translation="MSNGADKPEVAKWDPGFTRQIANWVGPVIKRYFRAEVRGLESIP
                     KAGGALVVSNHSGGMFTPDVLVFAPAFYEKFGFDRPVYTLAHYGVFIANIGDWLRRAG
                     VIEASRENAAQALRDGAVVLVFPGGDYDSYRPTWTENVIDFGGRTGYVRTAIETGVPI
                     VPMVSIGAQETQLFLARGDSIARRLGLKRARVEILPISFGFPFGLSAVLPPNVPLPAK
                     IVTRVLEPIDIAAEFGDDPDVDEVDLHVRAVMQEALDELAKQRRFPVLG"
     gene            complement(3187..4617)
                     /locus_tag="VFC01_10890"
     CDS             complement(3187..4617)
                     /locus_tag="VFC01_10890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZN80177.1"
                     /translation="MKRLNGMDAMLLYSETPNLHTHTLKVAIINAADFDGEFTFDVFR
                     RTIARRLHLLDPLRYQLVDIPWKLHHPMWLENCPVDLDYHLRRVRVPSPGGRRELDEV
                     IGQIASTPLDRGRPLWEFHFAEGMADDRFALIGKVHHTLADGVASANLLARLMDIDGS
                     AQNERDDYDMCNPPTKAELLRAAGRDHVQNVVALPGLIRDAVRGISRVRRHLKGRNHH
                     PDMAKMFKTPPTFVNHVVSPGRTFATASVSLADVKETAKHLGVTFNDIILAMSAGGLR
                     ELLLRYDGRADQPILASVPVSTDRSPDRITGNEISGLAVSLPVHIDDPLERVRLTALA
                     TARAKEENDLFGPTLHGRMMEYLPPPLTPALFRWQSKRASRSMIMNVAVSNVPGPREH
                     GHVGGATVSEIYSIGVLSAGSAFNMTVWSYVDQVDISVLSDDRTFNDVHEATAAMVHG
                     LAEIRRAAGLSELTPVDTAMAPASAG"
     gene            complement(4721..5557)
                     /locus_tag="VFC01_10895"
     CDS             complement(4721..5557)
                     /locus_tag="VFC01_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZN80178.1"
                     /translation="MAGRVEGKVAFITGAARGQGRAHAVRLAAEGADIIAIDICKQID
                     TVQIPLSTPEDLAETADLVKAHDRRIYTAEVDVRDFDALKAAVDAGVEQLGRLDIIVA
                     NAGIGNGGATLDKTSEPDWTDMIDVNLGGVWKTVKAGVPHILAGGRGGSIILTSSVGG
                     LKAYPHTGHYVAAKHGVVGLMRTFAVELGAQNIRVNSVHPTNVNTPLFMNEGTMKLFR
                     PDLENPGPDDMKVVGQLMHTLPIGWVEPEDIANAVLFLASDEARFITGVTLPVDGGSC
                     LK"
     gene            complement(5591..6787)
                     /locus_tag="VFC01_10900"
     CDS             complement(5591..6787)
                     /locus_tag="VFC01_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN80179.1"
                     /translation="MTVSAESDVYFDPYDVQLNADPYPMFRRLREEQPLYYNAQHDFY
                     ALSRFSDVNKALVDHETFSSARGAIVELIKANIDIPPGTVIFEDPPIHDIHRKLLARM
                     FTPRKINALEPIIRQYCAESLDPLVGAKRFDFVADLGAQMPMKVISALLGIPEDDQEM
                     VRDHANAQMRTEAGQPMKADGFVTGELFETYIDWRVDHPSDDIMTELLNAEFADETGT
                     TRHLTRQELLTYLNVVAGAGNETTTRLIGWAGKVLAEHPEQRRELVENPALIPQAVEE
                     LLRFEPPAPHVARYVTRDVEYYGTTVSEGSVMMLLIGAAVRDHRQFPPDGDVFDIHRE
                     QRQHLAFSVGTHYCLGSALARLEGRIALEEILKRFPEWDVDMTTATLSPTSTVRGWES
                     MPALLP"
     gene            complement(6929..8005)
                     /locus_tag="VFC01_10905"
     CDS             complement(6929..8005)
                     /locus_tag="VFC01_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007538332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN80180.1"
                     /translation="MHANPLPASLPDDQSTREYRVVQWSTGTIGARALRAVIEHPHMS
                     LAGVYVHGQDKVGRDAGELCGTASTGVTATDSIDDIVELGPDCVLYMPSALNLDELCR
                     LLAAGINVVTTCGAFHHPASMDPEIRAQVQAACEVGGTAVHSTGSSPGFISEAVPLVL
                     TSIQRQLNALTIDEYADLSQRNSPEMLFGLMGFGRPIGPFEQFRADYLRSSFGPSLQL
                     VADAIGLPLDSVEASGELAAASEDTTIAAGTIKAGSVAAQRITVAGIRGGRLLIQFRA
                     TWYCTTELAPVWELLDTGWHVSVDGDAPLEVTLRMPIPLERMADISPAYTANRAVNAV
                     PYVCAAAPGIHSTVDLPQVVTRLR"
     gene            complement(8008..8829)
                     /locus_tag="VFC01_10910"
     CDS             complement(8008..8829)
                     /locus_tag="VFC01_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HZN80181.1"
                     /translation="MPDLWSDLLACLDLHPVPSGGTGEGVPTEFEGRNQRLEYHRVFG
                     GQLLGQFIRIASVICPDKAVKSQHAVFAREGRADEPIRYQAIRHHDGRSFGTITITAR
                     QSRGVVATSSICMHAAEDGPERQTVDDVPAVLGADHQVALDLIPWETRAAVDLNATTT
                     GPPQFEVWMRTPAVDATLAPALAAYGTDLTLIGTALRPMDGVSQRGNGTWFTSAVTSH
                     TIWFHRPFRTDAWFLLRQHSPLLAHGRSFGRGDILTEDGTLVASYAQEALLRFAS"
     gene            complement(8879..9595)
                     /locus_tag="VFC01_10915"
     CDS             complement(8879..9595)
                     /locus_tag="VFC01_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80182.1"
                     /translation="MARRSPVQSVHVLPNRPASEPPVTSPSEEPAWKQRAVERSIKTA
                     KLRAAQRVQRFLDAAQAIIIEKGSTDFTVQEVVDRSRQSLRSFYLQFDGKHELLLALF
                     EDALSRSADQIRAAVSSQKDPLERLKVAVTLLFESSRPDPTAKRPLFTDFAPRLLVSH
                     PSEVNMAHAPLLALLTELMEAAAEAGKLRKGINPKRMAAMTMQTVMFVSQHSGDEEGS
                     GHPITADEVWDFCSHGFAAS"
     gene            9714..10889
                     /locus_tag="VFC01_10920"
     CDS             9714..10889
                     /locus_tag="VFC01_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80183.1"
                     /translation="MQLTFDADVEAFRTEFVAFLDEHLPAEAEALERSLSSSHVPQWA
                     RHWQRLLFDNGWLLPGNPPEFGGRNATLLQQYVHQEELARRRVYLTFNPQGVGIISAS
                     LLSFGTPEQQRRWAVPILRAEMTASLGMSEPGAGSDLASLRTRAARDGDDFVVNGQKV
                     WTSGAHDADVLLTFVRTDPNAPKHKGISALMIPTDLPGVVRRPFASACDQDDLDFNEV
                     FFNDVRVPAENLIGPLNEGWRVANGSLGHERNMLWLSYADRLQEFVEDFRPRTVLDRD
                     RYATLVMDNQALRLLGSEALARAARGEEDAAALSVLKLFGSEASQMASEYALTAAGVD
                     GLAHPSMSGPYSAHHLDLYRSGWFERYVRSFGGTIAGGTSEIQRNIIAQRVLGLPRN"
     gene            10899..11681
                     /locus_tag="VFC01_10925"
     CDS             10899..11681
                     /locus_tag="VFC01_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZN80184.1"
                     /translation="MYIDYDVADRIATITLNRPEAANAQNAELLDELDAAWTRAAEDN
                     DVSVILLRANGKHFSAGHDIRGGGPVPDKVTLELIIEHEAKRYLEYTLRWRNVPKPSI
                     AAVQGRCISGGLLLCWPCDLIVAADDAQFSDPVVLMGIGGVEYHGHTWELGPRKAKEI
                     LFTGRAMTAEEVLSTGMVNRVVPRDQLDSETRALAEQIAKMNPFALRQAKRAVNQTLD
                     VQGFYAAIQSVFDIHQTGHGNALSVSGWPVLVNLDEMKANIQ"
     gene            complement(11757..12497)
                     /locus_tag="VFC01_10930"
     CDS             complement(11757..12497)
                     /locus_tag="VFC01_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80185.1"
                     /translation="MLSTSGAELDAALADLGWADMLSDMPDLAIPLVFRLLGETGSHA
                     SVLNDVLLETIGGLPGGTPPMPYAGGGWVVWERTGTSDTTLGGLPLRRVPDGELMRVG
                     EARRATGWWLVGSARAMLALARQHALDRVQFGRPIAAFQAVRHRLAETLVAIEGAEAT
                     LSLPGTDSPDLTAMLAKAAAGKAALTAARHCQQVLGGIGFTAEHDLHHHVKRALVLDG
                     LLGSSRELTRKAGAGLRARGSAPRLASL"
     gene            complement(12548..13666)
                     /locus_tag="VFC01_10935"
     CDS             complement(12548..13666)
                     /locus_tag="VFC01_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80186.1"
                     /translation="MTVPSTEEFRSQLVAWLDDNDLTPPDDQSLSGHVRQFARVQRAL
                     YDAGWSRYGWPEHAGGLGGPAMLRAIVGEEVVGRRLAEPGPYSMLEVLAPTMIDYAPA
                     ELAAEMVPKLLSGEEQWCQGFSEPGSGSDLASLTTRAVQRGDQWIINGQKVWTSFAQY
                     SHRCILLTRTGDADTPNHEAITAFFVDLDTPGITVRPLRTMHDVDEFCEVYFDDVAVD
                     ASRMLGKPGDGWRLAMDLLPYERSTCFWQRIAYLYSRFDALIDEVKRQGQSVDSDLGE
                     AYLALHTLRCRSRATQRRLADGHKLGPDTSIDKVLLAGAEQRLYDTARDLLPGAIELD
                     DTEWRTEFLYSRAATIYGGTAEVQRNIIARRLLDLGKE"
     gene            complement(13663..14097)
                     /locus_tag="VFC01_10940"
     CDS             complement(13663..14097)
                     /locus_tag="VFC01_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN80187.1"
                     /translation="MTQSRIEDLVEIQQLLAKYAVTITQGDIDGLISVFTPDGTYSAF
                     GSTYTLNRFPELVDAAPKGLFMTGTSLVTLDPDNPDEATGTQPLCFIEHSKHDMRIGY
                     YNDTYVRTADGWRLKTRAMTFIRRSGEHDSGRPHAIGRPEAG"
     gene            complement(14078..15301)
                     /locus_tag="VFC01_10945"
     CDS             complement(14078..15301)
                     /locus_tag="VFC01_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN80188.1"
                     /translation="MLTLKAAGLVDVDAGEIVRPGIVRVEDDRIVGIGGDVEGDVIDL
                     GDSILLPGLMDMEVNLLMGGRGENPGLSQVQDDPATRVLRAVGNARRTLRAGFTTVRN
                     LGLFVKTGGYLLDVALGKAIDAGWIEGPRVIPAGHAITPTGGHLDPTMFAAFMPGVLE
                     LTVEEGIANGVDEIRKAVRYQIKHGAQLIKVCCSGGVMSLTGEAGAQHYSDEELRVIV
                     DEAHRRGLRVAAHTHGAEAVKHAVECGIDCIEHGFLMDDEAIKMMVDNDRFLVTTRRL
                     AQAMDVSKAPKELQDKAAEMFPKAETSIKAAYEAGVKIAVGTDAPAIPHGKNADELVT
                     LVEWGMPPDAVLRAATVVAADLINKSDLGRIAEGYLADIIAVPGDPLDDVTVTQKVNF
                     VMKGGKVFRHDAVTN"
     gene            complement(15304..15633)
                     /locus_tag="VFC01_10950"
     CDS             complement(15304..15633)
                     /locus_tag="VFC01_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80189.1"
                     /translation="MSKLPEEFADLERFTDWCLPTEEQRYQKRLNSTMAEMQDLYDAG
                     MARLEDIMVYVDARFPLQGMPEDAKALVHLGQSIVMVSFPVEVWKQPRVLDSGASYIQ
                     LIKEPVV"
     gene            complement(15626..16960)
                     /locus_tag="VFC01_10955"
     CDS             complement(15626..16960)
                     /locus_tag="VFC01_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZN80190.1"
                     /translation="MAHFPKPAAGSWTENWPELGTAPVNYEDSIDPEHFKLEQQAIFK
                     KTWLNVGRVERLPKKGSYFTREMPSVGPGTSVIIVKDKQDVVRAFYNMCRHRGNKLVW
                     NDYPGEEVSGTCRQFTCKYHAWRYSLEGDLTFVQQEGEFFDLDKSQYGLVPVRCEVWE
                     GFIFINFDNDAAPLTEYLGDFAKGLEGYPFHEMTEHYSYRSEINANWKLFIDAFVEFY
                     HAPILHMKQAEKEEAEKLAKFGFEALAYDIKGIHSMVSSWGGMSPPKDLSMVKPIERI
                     LHSGLFGPWDRPDIKGILPDELPPAINPSRQPTWGTDSFEFFPNFTLLFWAPGWYLTY
                     HYWPTAVDKHIFEADLYFVPPKNTRQRLSQELAAVTFKEYAFQDANTLEATQTQIGTR
                     VVTDFPLCDQEILLRHLHKTAWDFVNAYKNNGNGSANGKAQEKTSEKDSLNV"
     gene            complement(16967..17497)
                     /locus_tag="VFC01_10960"
     CDS             complement(16967..17497)
                     /locus_tag="VFC01_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN80191.1"
                     /translation="MRVPPLPEDQWDDAVDHALSGLLPAERRNPEAAGNLLATLVRHP
                     KLTRAFLRFNNHLLFSSTLPPRLRELAVLRVAHLHGSEYEWRHHVRMGREAGLTDDVI
                     DGIQRGEASDEFDQAILRAVDELQDKSVISDATWATLSEHLDERQRMDLVFTIGCYGA
                     LAMAINTFGVEPDQER"
     gene            17748..>18598
                     /locus_tag="VFC01_10965"
     CDS             17748..>18598
                     /locus_tag="VFC01_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN80192.1"
                     /translation="MNKDDMILISVDDHVVEPPDMFKNHLPKKYLDEAPRLVHNPDGS
                     DTWQFRDVVIPNVALNAVAGRPKEEYGLEPQGLDEIRPGCWKVDERVKDMNAGGILGS
                     ICFPSFPGFAGRLFATEDAEFSLALLQAYNDWHVEEWCGAYPARFIPMCLPVIWDAEA
                     CAKEVRRNAERGVHALTFTENPAAMGYPSFHDDYWTPLWEALVDTDTVMNVHIGSSGR
                     LAITAPDAPMDVMITLQPMNIVQAAADLLWSKPIKKYPDLKIALSEGGTGWIPYFLER
                     ADRTYEM"
BASE COUNT         3178 a         5824 c         6065 g         3531 t
ORIGIN      
        1 ttatcaacgt gacgtcgatc gaaggcgtaa gggcggcacc cgggtatgcg gcgtacgccg
       61 cggccaaagc cggcgtcatc aactacacca agactgcggc tctcgagtca acgcgctcgc
      121 ccccgatcta accgtgaccg aaggcctgct ccgcatctca cccgacggtt tgtcgcccaa
      181 cgccggccac atgattccga tggggcgacc aggcgatgtc gacgaaatgg cgggcgcggc
      241 agtatttctg gcgtcaaaca tgtccagcta catcaccggt cagaccatcc acgtcgacgg
      301 cggaacccag gccgcgagcg gctggtacca caacccgcag actggcgact accagctggg
      361 acccggttag tcagtgggac cagccctcgg ccgcctgttc gtcaaacgtg cggtcgatgc
      421 gctcgaagcg gcgccggatc gaatcccgtg ccgcgcggtg cggtagcacg tagaggcgat
      481 tggccagaat cgcgtcggcc gtcagccgcg cgacgtcctc gacgttgagc accgaatcgt
      541 cgtccggcgc ggatgccagc tcctggacgg tctcggcgtc ggagtgggcc ggcccgtagt
      601 ccgcgctcct gacgcgttcg gtattcgcca acaggttggt ttcgacgatc atcgggcaca
      661 gcaccgtgac gccgatgccg ttgggcctcg cctcccgggc cagggtctcg gccagcgcaa
      721 cgacgccgta cttcgccaca cagtaggggc cgagccccac gttcgggatc agacccgcga
      781 acgacgacgt gaacaccatg tggctgccgg agcctaaccg cggcaggaac gcctcgacac
      841 cgtggatcgg gccccagagg tcgacgtcga tcacgaaccg ccagtcgtca tggctcgttt
      901 ccgcgatcgg gccggcgtag gcaatgccgg cgttgttgaa caccacatgc acgtcaccga
      961 agacgcgaaa cgcctcatcg gccagatgat tcacgtcgtc gagcttgcgc acatcgcaca
     1021 ggacaccgtg tgcgtcgatc ccgtccgcgc ggagggtcgc gaccgcgtcg tcaagcgcgg
     1081 attgatcgat atcggcgagc atcaccctgg cgccgcggcg agcgaattct cgtgcggtgg
     1141 cgaagccgat gccgttggcc ccgcccgtga tgaccgttgc gcgtccgtcg taactctcca
     1201 cggcgcgaac cctatgcgct tagccgcggt cgtacggccg atcccacgat ggatcgccca
     1261 atttggtgca tatggcgccc catggatcgg cgtacttgcc cggcactttc caatacgcgc
     1321 cgcggcgctt catgacctcc cgggcgatcg gcacgatcag ccgcgccgga tagcgtttcc
     1381 atccgaattg cgcacgcagc tccgtcatca tgtcgggcca cgaatagtgc tggaacttca
     1441 aggcttcctg cgcccgggtg gtgtccatcc aatcggtcac gaaccagtcg tcgtcgctgt
     1501 cggggttacc gggtcgtccc ggtggcactg caccgggcat gcccagcgcc gaggcgaccg
     1561 ctggtgtgag gtcgccgtag cgccttcggt gtgactcgtc gcccgcgatt agcaggattt
     1621 cgcgtgcgac gtcggccgtg gtcgccgccg cgcacgccca tgcgacgtcg cggacgtcga
     1681 cggtctgcac ccggttgtcg ctgggcagtg cgctctcgaa gaacattgcg tcggtgctga
     1741 gcggcagcgc cgacagatcg atgctgagca cgccgccgaa ccgcaaaacc acccattcca
     1801 aagtggacga ctgcacgatc tcctcggcct ctgccttggt tccgctgtag atgtcgcagg
     1861 gccgcatcgg gtcgtccgcc cgaagcggct cgggcgtgcg gtgcgggttt cgcggcccga
     1921 acaccgcatt gctcgacgcg tggacaaatc gcggtggatt cggttgcgcc tcggcgatac
     1981 ggaccaaggt ctcggtcgca tccacattga cccgtcgcgc gatctgcggc acccggtaga
     2041 tcgccggcgc gatgatcgcg gcgaggtgga tgatggccgc cggtgcgacg tcggaaatga
     2101 cacgctgtac ttggtccgga tccgtcaggt cggtccaccg gacttcgacc ccgccgggga
     2161 gcgccttgat cttcttgacg ttggcgggtg attcgagatc ggctacgacg acgcgccgcc
     2221 catcggccgc caatttccct accgtcgccg accccaccag gccaaagccg cccgtgacca
     2281 gtaccgtgtc tgccattaag gctccctacc tgcggatata gtattctctt ttttcgagag
     2341 tatcatactc tgattgtggg gtatcgaagc ggggaacagg cacgacatga gcaacggggc
     2401 ggacaagcca gaggtcgcca agtgggatcc gggattcacc cggcagatcg caaactgggt
     2461 ggggccggtc atcaagcggt acttccgcgc cgaggtgcga ggcctggagt cgattccgaa
     2521 ggccggtggt gcactggtcg tgtcgaacca ttccggcggc atgttcaccc ccgacgtact
     2581 ggtgtttgcg ccggctttct acgagaagtt cggctttgat cggccggtct acacactcgc
     2641 gcactacggc gtcttcatcg ccaacatcgg tgattggttg cgtcgtgccg gggtcattga
     2701 agccagccgc gaaaatgccg cccaggcatt gcgagacggc gcggtggtgc tcgtctttcc
     2761 cggcggcgac tacgactcgt accggccgac ctggacggaa aacgtgatcg acttcggtgg
     2821 ccgcaccggc tacgtcagga cggccatcga aactggcgtg ccgatcgtcc cgatggtgtc
     2881 gatcggcgca caggaaaccc aattgttcct tgcccgtggc gattccatcg ccaggcggct
     2941 ggggctcaag cgagcgcgcg tggagatact gccgatcagc tttgggttcc cgtttggcct
     3001 gagtgcggtc ttgccgccga atgtgccgct gcccgccaag atcgtcaccc gagtgctcga
     3061 gccgatcgac atcgctgcgg agtttggcga cgaccccgac gtcgacgagg tcgatctcca
     3121 tgtgagggcg gtcatgcaag aggccctcga tgagctggcg aagcagcgcc gattcccagt
     3181 gctgggttag ccggcggacg cgggggccat cgcggtgtcg accggcgtga gctcggaaag
     3241 acctgcggcg cgccggattt cggccagccc atggaccatc gccgcggttg cctcgtggac
     3301 atcgttgaac gtgcggtcgt cggacagcac cgagatgtct acctggtcaa cataactcca
     3361 cactgtcatg ttgaatgcgc ttcccgccga cagcacaccg atggagtaga tctcgctgac
     3421 cgtggcgccc ccgacatgac cgtgctccct aggtcccggc acgttggaca ctgcgacatt
     3481 catgatcatg ctgcgagagg cccgctttga ctgccatcgg aacaacgccg gtgtcaacgg
     3541 tggcggcaga tactccatca tccgtccgtg caatgtcgga ccgaaaaggt cgttttcctc
     3601 cttggcacgt gcggtggcca gtgccgtcag ccggacccgc tccagcggat cgtcgatgtg
     3661 gacgggcagc gaaaccgcca atccactgat ttcgtttccg gtgatccgat ccggcgacct
     3721 gtcggtgctg acgggaacgg atgccaggat cggctggtcg gccctgccgt cgtagcgcaa
     3781 cagcagctct cgcaggcctc cggctgacat cgccagaatg atgtcgttga acgtgacgcc
     3841 gaggtgtttc gcggtctcct tgacatcggc aagcgacacc gatgcggtcg cgaacgtccg
     3901 gccgggcgag accacatgat tgacgaatgt cggtggtgtc ttgaacatct tggccatgtc
     3961 cgggtggtgg ttgcgccctt ttaagtggcg ccgtacccgg gagatgccgc gaacggcgtc
     4021 ccttatcaat cccggcagcg cgacgacatt ctggacgtgg tcgcggccgg ccgcgcggag
     4081 caactcggcc ttcgttggtg ggttacacat gtcgtagtcg tcgcgctcat tttgtgccga
     4141 tccgtcgata tccatcagcc gcgcgagcag gttcgccgag gcgaccccgt cggccaacgt
     4201 gtggtgcacc ttgccgatga gtgcaaagcg gtcgtcggcc atcccctccg cgaaatgaaa
     4261 ctcccacagc gggcgacccc gatcaagcgg agtgctggcg atctgcccga tcacctcgtc
     4321 gagctcccgg cgccctccag gactgggcac ccgcacgcga cgcaggtgat agtcgaggtc
     4381 gaccgggcag ttctccagcc acatcgggtg atgcagtttc catggaatgt cgacgagctg
     4441 gtagcgcagc gggtcgagca gatgtaaccg ccgggcgatg gtccgccgga acacgtcgaa
     4501 ggtgaactcg ccgtcgaagt cggcggcgtt gatgatcgcc accttcaacg tgtgggtgtg
     4561 caggttcggc gtttcgctgt acagcagcat ggcgtccatg ccgttgagtc gcttcacatg
     4621 cctcccccgc ggtcaacggt tctatcgaac caccgaccgc gaggcgttcc gggcggtttc
     4681 ggtgaatgac cctatttgtc gatcgcgtcc agcgcgacgg tcacttcagg cagctgcccc
     4741 cgtcgaccgg aagcgtcaca ccggtgatga agcgggcctc gtcggatgcg aggaacagca
     4801 ccgcgttggc gatgtcctcc ggctcgaccc agccgatcgg cagcgtgtgc atcaactgtc
     4861 cgacgacctt catgtcgtcg gggcccgggt tctcgagatc gggacggaac agcttcatcg
     4921 tgccctcgtt catgaacagc ggggtgttca cgttcgtcgg gtgcacagag ttgacgcgga
     4981 tgttctgcgc tccgagttcg accgcgaacg tccgcatcag gcccaccaca ccatgtttcg
     5041 cggccacgta gtgacccgtg tgcgggtagg ccttcaggcc gccgaccgag ctggtcagga
     5101 tgatagagcc gccgcggccg cccgcgagga tgtgggggac accggctttg acggtcttcc
     5161 agacaccgcc caggttgacg tcgatcatgt cggtccagtc cggttcactg gtcttgtcga
     5221 gggtcgcgcc gccgttgccg atgccggcgt tggcgacgat gatgtcgagc cgcccgagtt
     5281 gttcgacgcc ggcgtccacc gcggccttca gcgcgtcgaa gtcacggacg tcgacttcgg
     5341 cggtgtagat cctgcggtcg tgcgccttga ctagatcggc ggtctcggcc agatcttccg
     5401 gtgtggacag cggaatctga acggtgtcga tctgtttaca gatgtcgatc gcaatgatgt
     5461 cggcgccttc ggcggccaac cgcaccgcgt gcgcacggcc ctgtccgcgc gccgccccgg
     5521 tgatgaaagc gaccttgcct tcaacacgtc cagccatggt tacctcaatt cgtaattagt
     5581 ggaagggatt tcacggaaga agggccggca tcgattccca gccacgaaca gtggacgtcg
     5641 gtgacagtgt cgcagttgtc atgtcgacat cccattctgg gaaccgcttg aggatctcct
     5701 cgagcgcgat gcgtccctcg agtcgggcca acgccgagcc aaggcaatag tgcgtgccga
     5761 cgctgaatgc gagatgctgg cgttgctcgc ggtggatgtc gaatacgtca ccgtctggag
     5821 ggaattggcg atggtcgcgg acggccgcgc caatcagcag catcatcacg ctgccctcgg
     5881 acacggtcgt gccgtagtac tccacgtctc gggtcacgta ccgggcgacg tgcggggccg
     5941 gtggttcgaa gcgcagcaac tcctcgaccg cctgggggat cagtgccgga ttctcgacga
     6001 gctcgcggcg ctgttcgggg tgctccgcca acaccttgcc ggcccacccg atcaatcgcg
     6061 tggtggtctc gttgcccgcg cccgccacca cgttgaggta ggtcagcagc tcctggcggg
     6121 tcaggtgacg cgtcgtaccc gtttcgtcgg cgaactccgc gttgagcagt tcggtcatga
     6181 tgtcgtccga cgggtgatcg acgcgccagt cgatgtaggt ctcgaacagt tcgccggtga
     6241 cgaagccgtc ggctttcatc ggctgacccg cctcggtgcg catctgcgca ttggcgtggt
     6301 cgcgcaccat ctcctggtcg tcttcgggga tgcccagcag tgcactgatc accttcatgg
     6361 gcatctgcgc gccgaggtct gcgacgaagt cgaatcgctt ggcgcccacc agagggtcaa
     6421 ggctttcggc acagtactgg cggatgatgg gctcgagcgc gttgatcttg cgcggcgtga
     6481 acatccgggc gagtagcttg cgatggatgt cgtggatcgg cggatcttcg aaaattactg
     6541 tgccaggcgg gatgtcgatg ttggccttga tcagttcgac gatcgcacca cgagccgagc
     6601 tgaaggtctc gtgatccacc aacgccttgt tcacatcgct gaagcgactt agcgcgtaga
     6661 agtcatgctg ggcgttgtag tacagcggct gctcttcgcg aagccgacgg aacatcgggt
     6721 acgggtcggc gttgagctgg acgtcgtacg gatcgaagta gacatcgctt tcagcgctga
     6781 ctgtcacggt gtcacctctc gctgggccgg agttgcgtaa gacaaattgc tgccatttgt
     6841 ctcacaactc tggcatcgcg cccgagtggg tgtcaataag agactcattt ctgacaattt
     6901 aagctctccg cttagcagaa cgggcttctc atctgagtct ggtgacgacc tgcggcaggt
     6961 ccaccgtcga gtggatccca ggtgctgccg cgcagacata cggcacggcg ttgacggccc
     7021 ggttggccgt gtacgccggc gagatgtcgg ccatgcgctc gagggggatg ggcatgcgga
     7081 gcgtgacctc cagcggcgcg tcgccgtcga ccgacacatg ccatccggtg tccagcagtt
     7141 cccacactgg cgcgagttcg gtggtgcagt accaggtggc ccggaactgg atcagtagtc
     7201 gacccccacg aattcctgcg accgtgatgc gttgggccgc aacacttccc gccttgatgg
     7261 tgcccgctgc gatcgtggtg tcctcgctgg cagcagcgag ttcgccgctc gcttcgaccg
     7321 agtcgagggg cagtccgatc gcgtcggcaa ccaactgcag cgacgggccg aaacttgagc
     7381 gcaggtagtc ggcccggaac tgctcgaagg ggccgattgg gcggccgaaa cccatcaggc
     7441 cgaagagcat ctccggcgaa ttccgttggg acagatcggc atactcgtcg atcgtgagtg
     7501 cgttgagctg acgttggatc gaggtgagca ccagtggcac ggcctccgag atgaaccctg
     7561 ggctgctgcc ggtgctgtgc accgcagtcc cgccgacctc gcaggccgcc tgtacctgcg
     7621 cgcggatctc tggatccatc gacgccgggt gatgaaacgc tccgcaggtg gtgacgacgt
     7681 tgatgcctgc cgcgagcagt cggcacaact cgtcgagatt gagcgcgctc ggcatgtaga
     7741 gcacacagtc cgggccgagc tcgacgatgt cgtcgatgct gtcggtggcg gtcacgccgg
     7801 tggatgcggt gccgcacagc tcgccggcgt cgcggcccac cttgtcctgc ccgtggacgt
     7861 acacgcctgc caggctcatg tgcgggtgtt cgatgacggc ccgcagcgcg cgagcgccga
     7921 tggtcccggt ggaccactgg acgactcggt attcgcgggt tgattgatcg tcaggaaggc
     7981 tggcgggcaa cgggtttgcg tgcatgctca gctcgcgaac cgcaagaggg cttcttgggc
     8041 gtaggaggcg acgagggtgc cgtcttcggt gaggatgtcg ccgcggccga agctgcggcc
     8101 gtgggccagc agtgggctgt gctgccgaag caggaaccac gcatcggtgc gaaacggtcg
     8161 atggaaccag atggtgtggg acgtgacggc ggacgtgaac catgtgccgt tgcctcgctg
     8221 gctgacgccg tccatcggtc gcagcgcggt accgatgagc gtgagatccg tgccgtacgc
     8281 ggcgagcgcg ggtgcaagcg ttgcatcgac cgccggggtg cgcatccaga cttcgaactg
     8341 cggcgggcca gtcgtggtgg cgttgaggtc gacggccgcg cgggtttccc atggaatcag
     8401 gtcgagcgcg acttgatggt cggcgccgag gacggcaggc acgtcgtcga cggtttggcg
     8461 ctccgggccg tcctcggccg cgtgcatgca gatcgacgag gtcgctacga cgccgcgact
     8521 ctgccgggcc gtgatggtga tggtgccgaa cgatcggccg tcgtggtgcc ggatggcttg
     8581 gtatcggatg ggctcgtcgg cccggccctc gcgggcgaac accgcatgtt gggacttgac
     8641 ggctttgtcc ggacagatca ccgacgcgat tcggatgaat tgaccgagga gttgcccgcc
     8701 gaacacgcgg tggtattcga gccgttgatt gcggccctcg aactcggtgg ggacgccttc
     8761 tccggtgccc cccgacggca ccggatgaag atcaaggcat gcgagcagat cggaccagag
     8821 atcgggcact cactgagtgt aagcccgatt ctgcgttgat gagaacgtaa ttctcgtact
     8881 aactggcggc gaatccgtgc gagcagaagt cccagacttc gtccgcggtg atggggtggc
     8941 ccgacccttc ttcgtctccg ctgtgttgcg aaacgaacat gaccgtctgc atcgtcatgg
     9001 cggccatccg cttgggattg atgcccttgc gcagtttgcc tgcctcggcg gcagcttcca
     9061 tcagctctgt gagcagcgcc aacagcggtg cgtgcgccat gttgacctcc gagggatggg
     9121 agaccaggag gcggggggcg aagtcggtaa acagcggcct cttcgcggtc ggatcgggtc
     9181 gagaggactc gaacagcaac gtcacggcga ccttgaggcg ttccaggggg tctttctgac
     9241 tcgagaccgc cgcgcggatc tggtcggcgg accggctcag cgcgtcctcg aacagggcga
     9301 gcaagagttc atgcttaccg tcaaactgca ggtaaaagct tcgcagcgat tggcgcgacc
     9361 ggtcgacgac ctcctggacc gtgaagtcgg tgctgccctt ctcgatgatg atcgcctgcg
     9421 cggcgtccag gaaacgctgt acgcgttgtg cggctcggag cttcgctgtc ttgatcgaac
     9481 gttcgaccgc gcgttgcttc caagcgggtt cttcgctggg gctggtcacc ggcggctcag
     9541 acgcaggtcg attggggaga acatgaactg actgtaccgg agaacgcctt gccattgcgg
     9601 tcctcgaccc cctcgcggtt cacgtgtcag agattgtaac tttctcatca tgagaatagt
     9661 attctcattt tggagataac agtagtcttt cgatttttct tcccgggagc gccgtgcagc
     9721 tgaccttcga cgctgacgtc gaggccttcc ggaccgagtt cgtggccttt ctggacgaac
     9781 acctaccggc cgaggcggag gcgctggagc ggtcgctatc gagcagccac gtcccgcaat
     9841 gggcgcgcca ctggcagcgg ctgttgttcg acaacggttg gctgttgccc ggcaacccgc
     9901 ccgagttcgg cggccgaaac gccacgttgc tgcagcagta cgtccatcaa gaggagcttg
     9961 cgcggcgacg cgtgtacctg acgttcaacc cccagggcgt cggcatcatc tccgcgtcgt
    10021 tgctctcgtt cggaacgcct gagcaacagc ggcggtgggc ggtgccgatc ctgcgggccg
    10081 agatgacagc gtcgctcgga atgagcgagc cgggtgccgg ctccgatctg gcctccctgc
    10141 gtacgcgagc cgctcgcgac ggcgacgact tcgtcgtcaa cggtcagaag gtgtggacgt
    10201 ccggtgccca cgacgccgat gtgctgttga cttttgttcg caccgatcca aacgccccca
    10261 agcacaaggg catcagcgcg ttgatgatcc ccactgacct tcccggtgtg gtccgacgac
    10321 cgttcgcgtc ggcatgcgat caagacgact tggacttcaa cgaggtcttc ttcaacgacg
    10381 tgcgggtgcc ggctgagaat ttgatcgggc cactcaacga ggggtggcgg gtggccaacg
    10441 gctcacttgg acacgaacga aacatgttgt ggttgagcta cgccgaccgt ttgcaggaat
    10501 tcgttgagga ttttcgtcct cgtacggtgc tcgaccgtga ccgatacgcc acgctggtca
    10561 tggataacca ggcgttgcgg ctgctgggct cggaggcgtt ggcgcgcgca gcgcgcggcg
    10621 aggaggatgc ggccgcgttg tcggtgctca agctgttcgg atcggaagcc tcgcagatgg
    10681 cctccgaata tgcgttgacc gcagcaggag tcgacggcct tgcgcatccg agcatgtcag
    10741 gcccgtacag cgcgcatcac ctcgaccttt ataggtcggg atggttcgag cgctacgtgc
    10801 gttcgttcgg cggcaccatc gccggcggaa cttccgaaat ccaacgcaac atcattgccc
    10861 agcgcgtgct gggcttgccc cgaaattgag gaacaaccat gtacatcgac tatgacgtcg
    10921 ccgaccgcat tgcgactatc acgctgaata ggcccgaagc cgccaacgcc caaaatgccg
    10981 aactgctcga cgaactcgac gctgcgtgga cgcgcgccgc cgaggacaac gacgtctcag
    11041 tgatcctgtt gcgcgccaac gggaaacatt tctcggccgg ccacgacatc aggggcgggg
    11101 gaccggtacc cgacaaggtc acgctcgaac tgatcatcga gcacgaggcg aagcgctatc
    11161 tcgagtacac gttgcgctgg cgcaacgtcc cgaagccgtc gatcgccgcg gtccagggcc
    11221 ggtgtatttc cggtggcctg ctgctgtgct ggccctgcga cttgatcgtc gctgctgatg
    11281 acgcccagtt ctccgacccc gtcgtgctga tgggcatcgg tggcgtcgaa tatcacggcc
    11341 acacttggga attggggccc cgcaaggcca aagaaattct attcacaggt cgggcgatga
    11401 ctgctgaaga agtgctgagc accggcatgg tcaatcgggt ggtgccgcgc gaccagctgg
    11461 attcggagac gagggcgctg gccgaacaga tcgccaagat gaacccgttc gccttgcgcc
    11521 aggccaaacg cgccgtcaac caaacgctcg atgtgcaggg cttctacgcg gccatccaat
    11581 cggtgttcga catccaccag accggccatg gcaacgcgct gagcgtcagc gggtggccgg
    11641 tgctggtcaa cctcgacgag atgaaggcca acatccaata gctcacctcc ttcggcgagc
    11701 agacgcaaaa ctgccctatt tcggccataa atgggcaggt ttgtgtctgc tcgcgcctag
    11761 agcgaagcga ggcgcggcgc cgagccgcgg gcccgcagcc ccgctccagc cttcctggtg
    11821 agttcccttg agctgcccag caatccgtcg agcaccaggg cccgcttcac gtggtgatgc
    11881 aggtcgtgct cggcggtgaa gccgatgccg ccgagcacct gctggcagtg tctggccgcg
    11941 gtcaacgcgg ccttgcccgc tgcggccttg gccagcatcg cggtcagatc agggctgtcc
    12001 gtcccgggca ggctcagcgt cgcctcggcg ccttcgatgg ccaccagcgt ctcggccagc
    12061 cggtgccgga ccgcctggaa cgccgcgatc gggcggccga actgcacccg gtccagcgca
    12121 tgctggcggg ccagggccag catcgccctg gccgaaccga caagccacca ccccgttgcc
    12181 cggcgggcct cgcccacccg catcagctcg ccgtcgggca cccgccgcaa cggcagtcca
    12241 ccaagcgtgg tgtcgctggt cccggtgcgt tcccacacca cccagccacc gccggcgtac
    12301 ggcatgggcg gggttccgcc gggcaaaccg ccgatggtct ccagcagcac gtcgttgaga
    12361 accgaggcat gcgaaccggt ttcgcccagt agccgaaaca ccagcgggat cgcaagatcg
    12421 ggcatgtcgg acagcatgtc ggcccagccg agatcggcca gcgccgcgtc cagctcggca
    12481 ccggaggtcg acagcatggt tttacgcagt ccggcttcga gcagctcgag tgattcggga
    12541 tccacgatca ctccttgccg aggtccagca ggcggcgggc gatgatgttg cgttgcacct
    12601 cggcggtgcc gccgtagatg gtggccgccc gcgaatacag gaattcggtg cgccattcgg
    12661 tgtcgtcgag ttcgatggcg cccggcagca gatcgcgtgc ggtgtcgtag agccgctgtt
    12721 cggcgccggc caaaagcacc ttgtcgatcg aggtgtccgg ccccagcttg tggccgtcgg
    12781 ccaaccggcg ttgcgtcgcc cgagaccggc agcgcagcgt gtgaagcgcc agatacgctt
    12841 cgcccaagtc cgaatccacg ctctgccctt gccttttgac ctcgtcgatc agcgcgtcga
    12901 accgcgagta gaggtaggcg atgcgctgcc aaaagcacgt cgagcgttcg tagggcagca
    12961 ggtccatcgc cagccgccag ccgtcgccgg gctttccgag catccggctc gcatcgactg
    13021 ccacgtcgtc gaagtacacc tcgcagaact cgtcgacatc gtgcatggtg cgaagcgggc
    13081 gcacggtgat gcccggagtg tcgagatcga cgaagaacgc ggtgattgcc tcgtggttag
    13141 gggtgtccgc gtcaccggtg cgggtgagca ggatgcaccg gtgcgagtac tgggcgaaac
    13201 tggtccagac cttctggccg ttgatgatcc actgatcgcc gcgctgcacg gcacgagtgg
    13261 tcaacgacgc caggtcgctg cccgagcctg gctcggaaaa cccttggcac cactgctctt
    13321 cgccgctgag caatttgggc accatctcgg cggccagttc agctggcgca tagtcgatca
    13381 tcgtcggtgc cagcacctcg agcatcgagt acggtcctgg ctcggcaagc cggcggccca
    13441 ccacttcctc gccgacgatc gcccgtagca tcgccggacc gccgagcccg ccggcgtgtt
    13501 caggccagcc gtagcgactc cagccggcgt cgtagagcgc ccgctgcacg cgggcgaact
    13561 ggcggacgtg tcccgaaagc gactggtcgt cgggtggggt cagatcgttg tcgtcgagcc
    13621 aggccaccaa ctgtgaccgg aactcctccg tcgatgggac ggtcatccgg cttcgggcct
    13681 cccgatcgcg tgggggcgtc cggagtcgtg ttcgccgctg cgtcgaatga acgtcatggc
    13741 acgggttttc agccgccagc cgtcggcggt gcgcacgtag gtgtcgttgt aatacccgat
    13801 gcgcatgtcg tgtttcgagt gttcgatgaa gcacagcggt tgcgtcccag tggcctcatc
    13861 gggattgtcg gggtcgagcg tgaccagtga cgtaccggtc atgaacaacc ctttgggcgc
    13921 cgcgtcgacg agttcgggga agcggttcag cgtgtaggtc gatccgaatg cgctgtaagt
    13981 gccgtcgggg gtgaagacac tgatcagccc gtcgatgtcg ccctgcgtga tggtgacggc
    14041 atacttcgcg aggagctgct ggatctcgac caggtcctca attcgtgact gcgtcatgtc
    14101 ggaagacctt accgcccttc attacaaaat tgaccttctg tgtaacggta acatcgtcca
    14161 gcggatcccc gggcacggcg atgatatcgg cgaggtagcc ctcggcgatg cggccgagat
    14221 cggatttgtt gatcaggtcg gccgcgacga cggtcgccgc acgcagcacg gcgtcgggcg
    14281 gcatccccca ctcgaccagc gtgacgagct cgtcggcatt cttgccatgc gggattgccg
    14341 gagcgtccgt gccgacggcg atcttcactc ccgcctcgta ggcggccttg atcgatgtct
    14401 ccgccttcgg gaacatctcg gcggccttgt cctgcagctc ctttggagcc ttggagacgt
    14461 ccatcgcctg ggccagtcgc ctggtggtca ccaggaatcg gtcgttgtcg accatcatct
    14521 tgatggcctc gtcgtccatc aggaacccgt gttcgatgca gtcgatgccg cactcgacgg
    14581 cgtgtttgac cgcttccgca ccatgcgtat gagcggcgac gcgcagcccc cgccgatgcg
    14641 cctcgtcgac gatcacccgc agctcttcgt ccgaatagtg ctgtgcacca gcttctcccg
    14701 tcagcgacat gacgccaccg gagcagcaca ccttgatcag ctgcgcaccg tgcttgatct
    14761 gataccgcac cgccttgcgg atctcgtcca caccgttggc gatgccctcc tcgactgtca
    14821 gctccagtac gcccggcatg aacgcggcga acatcgtcgg gtcgaggtgc ccgcccgtcg
    14881 gggtgatcgc gtgaccggcg gggatcaccc gcggtccctc tatccacccg gcgtcgattg
    14941 ccttgcccaa cgcgacgtcg agcaggtaac cgcctgtctt gacgaacaat ccgaggttac
    15001 gaaccgtggt gaatccggcg cgcagcgtgc ggcgagcgtt gccaacggcg cgcagcaccc
    15061 gggtcgccgg atcgtcctgc acctgagaca gtccggggtt ctcgccccgc ccgcccatca
    15121 gcaggtttac ctccatgtcc atcaggccgg gaagcaggat ggagtcgccg aggtcgatca
    15181 cgtcgccttc gacatcgccc ccaataccga ctattcggtc gtcttcgaca cgaacgattc
    15241 ccggccggac gatttcgccg gcatcgacat caactagacc cgctgctttg agggtcagca
    15301 cgcttagacc accggctcct tgatcagttg gatgtatgag gcgccgctgt cgagcaccct
    15361 cggctgcttc cacacctcga cggggaagct caccatcacg atcgactgtc ccagatgcac
    15421 cagcgccttg gcgtcctctg gcatcccctg gagcgggaag cgcgcgtcaa cgtagaccat
    15481 gatgtcctcg agccgagcca tgcccgcgtc gtagagatcc tgcatctcgg ccatcgtcga
    15541 attgagacgc ttctggtagc gctgctcctc cgtcggcagg caccagtcgg tgaaccgctc
    15601 caggtcggcg aattcttctg gcagcttaga cattcagtga atccttctcg ctcgtcttct
    15661 cttgcgcctt gccattcgcg gaaccgttgc cgttgttttt gtacgcgttg acgaagtccc
    15721 aagcggtctt gtgcagatgg cgcagcagga tctcctgatc gcagagcggg aagtccgtga
    15781 cgacccgtgt gccgatctgc gtctgggtgg cttccagggt gttggcgtcc tggaacgcgt
    15841 actccttgaa cgtcaccgcg gccagttcct gagagaggcg ctggcgggtg ttctttggtg
    15901 gcacgaagta caggtcggcc tcgaagatgt gcttgtccac cgcggtaggc cagtagtggt
    15961 aggtgaggta ccaacccggc gcccagaaca gcagtgtgaa gttggggaag aactcgaaag
    16021 agtcagtgcc ccaggtgggt tggcgcgacg ggttgatggc cggcggcaat tcgtcgggca
    16081 ggatgccctt gatgtccgga cgatcccacg gtccgaacag gccgctgtgc aggatccgct
    16141 cgatgggctt gaccatgctc aggtccttcg gcggcgacat cccaccccag gacgacacca
    16201 tggagtggat gcccttgatg tcgtaggcca gcgcttcgaa gccgaacttg gccagcttct
    16261 cggcctcctc cttctccgcc tgcttcatat gcaggatcgg cgcatggtag aactcgacga
    16321 acgcgtcgat gaagagcttc cagttggcgt tgatctccga gcgatagctg tagtgctcgg
    16381 tcatctcatg gaacgggtag ccttcgaggc cctttgcgaa gtcgccaagg tattcggtca
    16441 gcggcgctgc gtcgttgtcg aagttgatga agataaaccc ttcccagacc tcgcaccgga
    16501 cgggcaccag gccgtactgc gacttgtcga gatcgaagaa ctcgccctcc tgctggacga
    16561 acgtcaggtc gccctcgagg ctgtagcgcc acgcgtggta cttgcaggtg aactggcggc
    16621 aggtgcccga cacctcctcg ccggggtagt cgttccacac cagcttgttg ccgcggtgac
    16681 ggcacatgtt gtagaaagcg cggaccacat cctgtttgtc cttgacgatg atcaccgacg
    16741 tgccggggcc gaccgatggc atctcgcgcg tgaagtaact gcccttcttg ggcagccgct
    16801 ccacccggcc gacattcagc caggtcttct tgaagatcgc ctgctgctcg agcttgaagt
    16861 gctccggatc gatggagtcc tcatagttca cgggcgcggt gccgagctct ggccagttct
    16921 ctgtccagct gccggcggcc ggcttcggga agtgtgccac tgtgtattac ctctcctggt
    16981 cgggttctac gccaaaggtg ttgatggcca tggcgagtgc gccatagcag ccgatcgtga
    17041 agacgaggtc catccgctgg cgctcgtcga ggtgttcgga caaggtcgcc catgtcgcgt
    17101 ccgagatcac cgatttgtct tggagttcgt cgacggcgcg caggatcgct tggtcaaatt
    17161 cgtcggacgc ctcaccgcgt tggatgccgt cgatgacgtc gtcggtgagc ccggcctcgc
    17221 ggcccatgcg gacgtggtgg cgccactcgt actccgagcc gtgcaggtgc gcgacgcgga
    17281 gcaccgcgag ctcgcgtagg cgcggcggca gggtcgacga gaacaacagg tggttgttga
    17341 agcgcaggaa ggcgcgcgtc agtttgggat gacggaccag cgtcgcgagg agattgcccg
    17401 cagcctcggg gttgcggcgc tccgcgggca gcaggccaga aagcgcgtgg tcgacggcgt
    17461 cgtcccactg gtcttccggc agcgggggca cccgcacggg gcgtctcctc tcactcacga
    17521 gaatcaggtt ctcatatttc gctaataggt ttccaccttt tctgcgaatg gtcaatgtga
    17581 agggccagaa ccgccggtca cagctgtgcc cgtggagggg caactgtgct gccagatggc
    17641 catggggtgg gtttcggacg ttgattctcg tactatgaga agatagtttc ctcagataga
    17701 gaacatcact ggcccaagac gcttcggcgt cgacggaagg aacaggcatg aacaaagacg
    17761 acatgatcct gatcagcgtt gatgatcacg ttgtcgaacc gccggacatg ttcaagaacc
    17821 atctgccgaa gaagtacctg gatgaggcgc cgcgcttggt gcacaacccg gacggatcgg
    17881 atacctggca gttccgcgat gtggtgatcc cgaatgtcgc gttgaacgcc gtggccggcc
    17941 gcccgaagga ggagtacggc ctggagcccc agggtttgga tgagatccgg ccgggctgct
    18001 ggaaggtcga cgagcgggtc aaggatatga acgccggcgg catcctgggc tcgatctgtt
    18061 tcccatcgtt cccgggtttc gccggtcggc tattcgcgac ggaggacgcg gagttctcgc
    18121 tggcgttgtt gcaggcctac aacgactggc atgtcgagga gtggtgcggg gcctatccgg
    18181 cgcgcttcat cccgatgtgt ctgccggtga tctgggacgc cgaggcctgt gccaaggagg
    18241 tgcggcgcaa cgccgagcgt ggtgtgcacg cgctgacgtt caccgaaaac ccggcggcca
    18301 tgggttaccc gtccttccac gacgattact ggacgccgct gtgggaagcg ttggtggaca
    18361 ccgacaccgt gatgaatgtg cacatcggct cgtcgggccg gttggcgatc accgccccgg
    18421 atgcgccgat ggacgtgatg atcacgctgc agccgatgaa catcgtgcag gctgcggcgg
    18481 atctgttgtg gtccaagccg atcaagaagt acccggatct caagatcgcg ctctccgagg
    18541 gcggcaccgg ctggattccc tacttcttgg agcgcgccga tcgcacctat gagatgca
//
LOCUS       DASRJA010000238         8627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4138666,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000238 DASRJA010000000
VERSION     DASRJA010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4138666"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFC01_10970"
     CDS             complement(<1..558)
                     /locus_tag="VFC01_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011595571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZN80193.1"
                     /translation="MSTRLSGVLTALATPFTPDGQIDESTLRRLVDRSIDGGVDGVVA
                     CGSTGEFAAMSGAERRLVVETVIDQAANRVPVVAQTGAVSTAEAVELSRHAEAAGASV
                     LMVVSPYYEPLSLDETVEYLRTVAAAVDIPIMLYNLPVATGVNLDPDTVGRLAREVDN
                     IRYIKDTSADMAQAGQLIHRHGDVIS"
     gene            complement(595..1986)
                     /locus_tag="VFC01_10975"
     CDS             complement(595..1986)
                     /locus_tag="VFC01_10975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN80194.1"
                     /translation="MKDVVIVGGGVAGLAAGWRLRHWDTVLLESGTRVGGRVRSERRG
                     PYFMNWGGHVYAGGNSSTAWLLNDTGVDSVAVPGSLSGLAMNGKLLLKGRIESYPFRI
                     PMPWSSRMGIVKTGAKVSLAVARYARIVGRRPGESEASRQQRVYSFMNDRSFADYIGD
                     LPEDVEALFKPTVTRSSADLDEISAGAGVGYFSLVWNIGAGLSQSIVGGPSTLTESIA
                     AALDGRVQLNSTVDEIVHKKNSVVVRYRQDGSEKEVEARCVVLATPATVSRRIAVDLD
                     RDVREALSKIVYGPYVAAAFLTNETERQVWDDAYAIATPKRSFNVLLNMSNIVRGYER
                     QRQPGSSIMTFSPASLARKLLELDDDQIRDTYLRDLDQILPHFSEKVVEAQVQRWPTG
                     APYCFPGRGKLQPTLTRRGGRVFLAGDYLGTLYTETAIGTGLTAAQEVQSLLATERQC
                     SPPIRLAPAVPLN"
     gene            2255..2938
                     /locus_tag="VFC01_10980"
     CDS             2255..2938
                     /locus_tag="VFC01_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011595305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN80195.1"
                     /translation="MSRRAEPRLLTDEVYAMIHEAIMSGDLPAGSRLKVRDLAAQVGT
                     SVLPVREAIRRLEEAGLAERSPHKGAVVKGLTLEELVHVYDVRRLLEVQAARSGAELI
                     SRQDCDKMQDEYDAMRAALTEGRVVAYLDHDEALLTILYKASGNPVLMELIRTLWNRC
                     RAYKIVGAQGSIDAGEDEPLWKFQLDLVAAARKNDAAAAAAVNDASLLNATDRIKERL
                     AAQAEAPKA"
     gene            3214..4557
                     /locus_tag="VFC01_10985"
     CDS             3214..4557
                     /locus_tag="VFC01_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN80196.1"
                     /translation="MTTAHPLDDAPLSRFHKKLALYASGGPFLDGYVLSIIGVALVQI
                     SPAFNMSSAQEGLVGASALVGIFLGAFLGGWLTDMFGRQVLFTIDLAAIILCSIAQFF
                     VGDVIWLIILRLLIGMAVGADYPIATSLLAEFSPRRWRGPLLGAFVAMWFVGAAAAYL
                     VGEILHHVDPINGWRWMLASAAVPAIIIVLARLGTPESPRWLLKKGRVDEANEVLRKV
                     FGAGVSVHDMPEEVHENLRVSALLKAGYGKRMAFITIFWTCSIVPLFAVYAFGPKILG
                     ALGLTGGMANYGSAFITVMFLVGCIVGLFLVDRIGRRPLIIHSFVWGGVALLLLGIFP
                     EANAWIVLALFAGYAVVIGGTQILQWIYPNELFPTEIRGSAVGLASSLSRIGAAIGTY
                     LVPISLSHLGTGGTMIVGAVVTFTGAVVSVMWAPETRGLTLGECAAISTKSKVSA"
     gene            4601..6109
                     /locus_tag="VFC01_10990"
     CDS             4601..6109
                     /locus_tag="VFC01_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN80197.1"
                     /translation="MTQTVQSPLTHVRRGNGAPLVSATDLPPRGHFIANEFTADAGGE
                     LLDIVNPADESVITQVLVGTADDVDRAVEAAIAAKSGWAQLVPKERAAILHQIADRVD
                     EHAELLARLEAANTGKPLTVARDDIAQTIDTFRFMAGAARSITSQAAGDYAEGHLSVI
                     VREPLGAIGVVTPWNYPLLMAAWKIAPILAAGNVLVLKPSEQTPLTTLKFAELVADVL
                     PAGVFNVVTGFGPVVGNRLSAHPQLDMIALTGSVNSGKAVARNASETLKRVHLELGGK
                     APVIVFDDADLAAAASALRTAGYWNSGQECGAGCRVLVHESVAAEFTEHLVAEVGSLI
                     VGEPGAGDDVEIGPLTSKAHFDRVCGFLDRARAEGVRAVLGGSAVAGPGYFVAPTVLT
                     DVPVGAECAREEIFGPVVTIETFTEETEAIERANSVPYGLAASVWTENARRGHDVPAR
                     LDFGTVWVNAHLVLASEVPWGGFKGSGYGRDLSIYALDDYSRTKHVMHNHTR"
     gene            6137..7060
                     /locus_tag="VFC01_10995"
     CDS             6137..7060
                     /locus_tag="VFC01_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZN80198.1"
                     /translation="MGIVKNDNRQFKLGMHSYTLHLNGCGESWGFEQDYAFEKTIDLT
                     KMMDLAVEWRLDVLHITLVDLDNDVSAEHLAEVKTAARSRGLDLELNVSINAPCDPRV
                     NASVEEALHIGHAIGAQLVKFSLDIKRPKPIYGSCMHPDVVVQLADRVYEFKKNIPLI
                     ENYGMKVALENHCDLFADEVIWLVEQLHHPLVGACLDTMNSLVLMEGTEECVKKMAPY
                     AYCCHFCDINIVVDADGTHSIGTPIGQGDIDCVMIMQELRANAPEALDTIVFEIEMPL
                     NGRSIEEGRELELRAAKESVNYMRSVLNVGH"
     gene            complement(7446..>8627)
                     /locus_tag="VFC01_11000"
     CDS             complement(7446..>8627)
                     /locus_tag="VFC01_11000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZN80199.1"
                     /translation="LAAAVFLLAAPAVATAEPNAAPEAPACPYRESTPPAVDSSEVPE
                     AGDPPAPLPVPAKPLGGDALSGCGIITAPGMPPVPGDISAEAWLVADLDSGDIVAARD
                     PHGRHRPASIIKVLIAMQSIKELPIHKVVAGTQDDANAEGTKVGVGENGFYSINDLLH
                     GLLMYSGNDAAHALAVQLGGMDTAIQKINDLAGKLGGRDTRAATPSGLDGPGMSTSAY
                     DIGLFYRYAWQNPTFADIVGTRTFDFPGRGDVGFPIENDNKLLVNYPGAMGGKTGYTD
                     DAGQTFVGAANHDGRRLVAVLLHGTREPIAPWEQAAHLLDYGFATAPGTKVGTLIEPD
                     PSLLAPKPDAAPNPAAAQASASLPDLDAMPVRVGVGIVGSIIVFGLILGARSLNRRPQ
                     H"
BASE COUNT         1546 a         2725 c         2737 g         1619 t
ORIGIN      
        1 cgagatgacg tcaccatggc gatgaattag ttggcctgct tgtgccatgt ctgcgctggt
       61 gtccttgatg tagcgaatgt tgtcgacctc gcgggcgagc cggccgacgg tgtcgggatc
      121 gaggttgaca ccggtcgcaa caggcaggtt gtacaacatg atcgggatgt cgaccgcggc
      181 ggcgacggtc cgcaggtact caacggtttc gtcgaggctg agcggctcgt agtacggcga
      241 gacaaccatc aggaccgacg ctccggcagc ctcggcatgc cgggagagct cgaccgcctc
      301 ggcggtgctc actgcaccgg tttgggcaac caccgggaca cggttggcgg cctggtcgat
      361 gacggtttcg acgacgagtc ggcgttccgc tccgctcatc gcggcgaatt ctccggtgga
      421 cccgcatgca acaacgccgt cgacgccgcc gtcgatgctg cggtcgacca gccggcgcag
      481 tgtgctttcg tcgatctgtc cgtcaggagt gaatggtgtt gcgagcgcgg tcagtacgcc
      541 actgagacgt gtggacatgt tgttcttctc cggtggtagt cgcgagtggt ggtttcagtt
      601 gagcggtacg gctggtgcca gacgaatggg tggtgaacac tggcgctcgg tggcaagcag
      661 actttggact tcctgcgcgg cagtcaatcc cgtcccgatc gcggtctcgg tgtacaaggt
      721 gccgaggtag tcgcctgcga ggaaaacgcg accgccgcga cgagtcagtg tgggctgcag
      781 cttgccgcgg cccgggaagc agtacggcgc ccctgtgggc cagcgttgca cctgcgcctc
      841 cacgaccttc tccgagaagt gcggcaggat ttggtcgagg tcgcgcagat acgtgtcgcg
      901 gatctgatcg tcgtcgagct cgagcagctt gcgcgccagg cttgcggggg agaaggtcat
      961 gatgctgcta ccgggctgcc gctgccgctc atagccgcgg acgatgttcg acatgttcag
     1021 cagtacgttg aatgaccgct tgggggtggc gatcgcgtag gcgtcatccc acacctggcg
     1081 ctcggtttcg ttcgtcagga acgcggcggc gacgtacggg ccatagacga tcttggacaa
     1141 tgcctcccga acatcgcgat cgagatcgac ggcgatcctc ctgctcaccg tcgcaggcgt
     1201 ggcgagcacg acgcagcgtg cttcgacctc cttctctgat ccatcctggc gataccgaac
     1261 aacgaccgaa ttcttcttgt gcacgatctc gtcgaccgtc gaattcagtt gcacgcggcc
     1321 atctaacgcc gctgcgatgc tttcggtcag ggtggatggt cctccgacga tgctctgact
     1381 cagacctgcc ccgatgttcc acacgaggct gaaatagccg acgccggccc cggcagagat
     1441 ctcgtcgaga tcggcagacg aacgcgtcac ggtcggtttg aacagagcct cgacatcctc
     1501 gggaaggtct ccgatgtagt cggcgaacga ccggtcgttc atgaagctgt agacccgctg
     1561 ctggcgggaa gcctcgctct cgccggggcg acgtccgacg atcctggcgt atcgggcgac
     1621 cgccagggac accttcgcgc cggtcttaac gattcccatg cgcgacgacc aaggcatcgg
     1681 gatacggaag ggatagcttt cgattctgcc cttgagcagc aatttgccgt tcatggccag
     1741 acccgacagc gagccgggca ccgcgaccga atcgacgccg gtgtcgttga gcagccacgc
     1801 tgtcgacgag ttgccaccgg catagacatg gccgccccag ttcatgaagt acggtccacg
     1861 gcgttcagag cgcacacgcc cgcccactcg tgtccccgat tcgaggagca cggtgtccca
     1921 gtggcgaagc cgccacccag ccgctaaacc ggcgacacca cctccgacga tcacgacgtc
     1981 cttcattggg ccgttctcct ctggttgctc aatgacgttg atgggtttgc tttttcgcgc
     2041 cttgatgcaa cggacacctg ttttgtgtac ttcgtcgcga gcgcgtcggg ctggtcctcg
     2101 gacggcgcgc cttcagggcg tatcagcccg agtctaggat gtgattacag atcacacaag
     2161 gcgtgactgt tgcttggctc acagcgaacc acgcgtcgac atgcgcggga aaccgggatc
     2221 gcgggtggtg ccacgtaaaa tgtgatctgt ggtcatatct cgccgtgctg agccaagact
     2281 gctgaccgac gaggtctacg cgatgatcca cgaagcgatc atgagcggtg atctaccagc
     2341 cggttcccgc ctgaaagtgc gggatctcgc cgcgcaagtc ggaaccagcg tgctaccggt
     2401 tcgtgaagcg atccgtcgcc tcgaagaggc gggattggcc gaacgctcac cgcacaaggg
     2461 tgccgtcgtc aagggcctga cgctcgaaga gttggtgcac gtctacgacg tacgtcggtt
     2521 gctcgaagtg caggcggcgc gcagcggtgc cgaactcatc agccggcagg actgcgacaa
     2581 gatgcaggac gaatatgacg cgatgcgcgc tgccctcacg gagggacgtg tcgtcgccta
     2641 cctcgatcat gacgaagccc tgctgacaat cttgtacaaa gcctcgggca atccggtgct
     2701 gatggaactg attcggacgc tatggaaccg ctgccgcgcc tacaagatcg tcggagctca
     2761 gggcagtatc gacgcgggcg aggacgaacc gctgtggaaa ttccaactcg atctcgtcgc
     2821 cgccgcgcgc aaaaacgacg ccgccgcggc ggcggcagtc aacgacgctt ctctgcttaa
     2881 cgcgactgat cgcatcaagg agcgcctggc cgcacaggcg gaagctccga aggcgtaggc
     2941 gattttcggc tgcctcgtgc gggcgaggtc ggcggccggg tgcagtggcg cctcgcagtt
     3001 gaaccgcaac gtcaacctcc ttcgaacaaa gagatcacaa atctcatctt gactgtaatc
     3061 tgtgatcaca tctaaagtgg tcggtatcac accaaacgcg acatgtggtc gacgccacca
     3121 gttgagtgac atcggccaac caaggaccgc cacctgatgg cgccaccgat atttccgagc
     3181 gaagactcag acccagaggg aaggaccagg tcaatgacca ctgctcatcc gctcgacgac
     3241 gcgccgttgt cgaggttcca caagaaactc gcgctgtatg cgtctggggg accattcctc
     3301 gacggctatg tgctgtcgat catcggtgtg gccctggtgc agatcagccc tgcgttcaat
     3361 atgtcctcag cccaggaagg cctggtaggc gcctcggcgc tggtcggcat cttcctgggc
     3421 gccttcctcg gtggctggct caccgacatg ttcggccgcc aggtgctgtt caccatcgac
     3481 ctagctgcca tcattctgtg ctcgatcgcc cagttcttcg tcggcgacgt catctggctg
     3541 atcatcctgc gactgctgat cggcatggcc gtcggcgccg actacccgat cgccacctcg
     3601 ctgctggccg agttctcccc ccgcaggtgg cgtgggccgc tgctcggcgc cttcgtggct
     3661 atgtggttcg tcggtgccgc agccgcctac ctcgttggtg agatccttca tcacgtcgat
     3721 ccgatcaacg gttggcgctg gatgctggcc agcgcggccg tgccggccat cattatcgtg
     3781 ctggcccggt tgggcacccc ggaatcccca cgctggctgc tcaagaaggg tcgcgtcgac
     3841 gaggcaaacg aggtgctgcg caaggttttc ggggcaggag tctcggtgca cgacatgccc
     3901 gaagaggtcc atgagaacct gcgcgtcagc gccttgctca aggccgggta tggcaagcgg
     3961 atggcattca tcaccatctt ctggacctgc tcgatcgtgc cgctgttcgc cgtctacgca
     4021 ttcgggccga agatcctggg agcactcgga ctgacgggtg gaatggccaa ctacggttcg
     4081 gccttcatca cggtgatgtt cctcgtcggc tgcattgtcg gacttttcct ggttgaccgg
     4141 atcggtcgcc ggccgctgat catccacagc ttcgtgtggg gcggcgtggc gctgctcctg
     4201 ctcggtatct tccccgaggc caacgcctgg atcgtgttgg cactgttcgc cggttacgcg
     4261 gtggtcatcg gcggtactca gatcctgcaa tggatctacc cgaatgagtt gttccccacc
     4321 gagatccggg gttctgccgt cggactggcg tcatcgctga gccggatcgg cgccgccatc
     4381 ggcacgtacc tggttcccat ctctctgtcg cacctcggca ccgggggaac catgatcgtc
     4441 ggcgccgtcg tcaccttcac cggcgcggtc gtgtcggtga tgtgggcacc ggaaacccgt
     4501 gggctcaccc tcggtgagtg cgctgcgatc agtaccaagt ccaaggtgag cgcttagcga
     4561 ccagcgcacc tgacccgctc cgttagaaag gcatgcttcg atgacccaga ctgtccagtc
     4621 tccgctcaca catgtgcgac gaggcaacgg tgctccgctc gtctcggcga cggacctacc
     4681 gccgcgcggc cacttcatcg ccaacgagtt cactgcggac gcaggtggtg aattgctcga
     4741 tatcgtcaac cctgccgatg agtcggtgat cacgcaggtg ctggtcggta ccgcagacga
     4801 tgtcgaccgg gccgtcgaag ccgccatcgc cgccaaaagc ggatgggcac agttggttcc
     4861 gaaggaacgc gccgcgatcc tgcaccagat cgccgaccga gtcgacgagc atgccgagct
     4921 gctcgcccgg ctggaggcgg ccaacaccgg caagcccttg acggtcgccc gcgacgacat
     4981 cgcccagacc atcgacacgt tccggttcat ggccggcgcc gcccggtcga tcacctcgca
     5041 ggccgccggc gactacgccg agggccacct ttcggtcatc gtgcgcgagc cactgggcgc
     5101 catcggagtc gtcactccgt ggaactatcc attgctcatg gcggcgtgga agatcgctcc
     5161 gatcctggct gctggcaatg tgctggtgct caaaccatcc gaacagactc cgctgaccac
     5221 gctgaagttc gccgaactcg tcgccgacgt gctgccagcg ggcgttttca acgtcgtcac
     5281 cggcttcggc ccggtggtcg gtaaccggct ctccgcgcac ccgcagctcg acatgattgc
     5341 tctcaccggc tccgtgaaca gcggtaaagc agtggcccgc aacgcttctg agactctcaa
     5401 gcgggttcac ctcgaacttg gtggcaaagc cccggttatc gttttcgatg acgccgacct
     5461 ggctgccgcg gcaagcgcgc tgcgcaccgc cgggtattgg aactccgggc aggaatgcgg
     5521 cgccggttgc cgggtgctgg tacacgaatc ggttgccgcg gagttcaccg aacacctcgt
     5581 cgccgaggtc ggctcgctga tcgtgggcga acccggcgct ggtgacgacg tcgagatcgg
     5641 accgctcact tccaaggcgc atttcgaccg cgtctgcggc ttcctcgacc gcgcccgcgc
     5701 cgagggcgtc cgcgcggtgc tcggcggatc ggcagtggcc ggacccggat acttcgtcgc
     5761 accaacggta ctcaccgacg tgcccgtcgg cgcggagtgc gcccgggagg agatcttcgg
     5821 tcccgtcgtc accatcgaga ccttcaccga agagaccgag gccatcgagc gcgctaactc
     5881 tgttccttac ggcctggccg catcggtgtg gaccgagaat gcccggcgcg gccacgacgt
     5941 tccggcgcgg cttgacttcg gcaccgtatg ggtcaacgcc catctggtgc tcgcgagcga
     6001 agtgccgtgg ggcgggttca agggttccgg atatggacgc gacctatcca tctacgctct
     6061 cgacgactac tcccgcacga agcacgtcat gcacaatcac acccgttgat cgacacgggc
     6121 gaaggggttc ttctcaatgg gcattgtcaa gaacgacaat cgtcagttca agctcggcat
     6181 gcactcatac acgctgcatc tgaacggatg tggtgagagt tggggattcg agcaggacta
     6241 tgccttcgaa aagacgatag atctcaccaa gatgatggat cttgcggtgg agtggagatt
     6301 agacgtactg cacatcacct tggtcgatct cgacaacgac gtcagcgccg agcatctggc
     6361 tgaggtcaaa acggccgcgc gcagtcgtgg ccttgacctc gaattgaacg tctcgatcaa
     6421 cgcgccgtgc gatccgcgtg tgaatgcctc ggtcgaggag gcattgcaca ttggtcacgc
     6481 catcggtgcg cagctggtga agttcagctt ggatatcaag cgtcccaagc cgatctacgg
     6541 ctcctgcatg catcccgatg tggtcgtcca gctggccgat cgcgtctacg agttcaagaa
     6601 gaacattccg ctcatcgaga actacggcat gaaggtcgcg ctggagaacc attgtgattt
     6661 gttcgccgac gaggtcatct ggcttgtcga gcagctgcat caccctctgg tcggcgcatg
     6721 tctcgacacc atgaactctc ttgtgctgat ggagggcacc gaggaatgtg tgaagaagat
     6781 ggctccctac gcttactgct gtcacttctg cgacatcaac atagtggttg acgcggacgg
     6841 cacccattca attggtacgc caatcgggca aggcgatatc gattgcgtga tgatcatgca
     6901 ggagctgcgt gccaacgcac ccgaggcgct tgacacgatc gtcttcgaga tcgagatgcc
     6961 gttgaacggc cgctccattg aggaagggcg cgaactcgaa ctgcgggccg ccaaggaaag
     7021 cgtcaactac atgcggagcg tgctgaacgt cggccactga tcagaccttt ttctgagcat
     7081 tacgggccga aggtgcgatg ccgcaaccgt gaatgcacgg gcgagtgcta gcacgttgcg
     7141 gccgccacgc ctgccgggca gtctcgcgcc tgccatcaat ccgcacaaga aacccacgac
     7201 gaaccaccca cgcgcgcgtc gcttgttcct tcgcctagcg gctgatgcga cgaatccagc
     7261 aaccgacgcg acgatcgcga ccgcgaaaag caaagcgatc agatgtgtcg gtccgagcga
     7321 ccccatatcg caacctccgg gccgtcgtct gcataggccg cttggccgat ccaaccaccg
     7381 tcaccgccgc agcagcacgg ccaagccgtg ttgtgaccaa agcgagccgg aagcgttagc
     7441 cgccactagt gctgcgggcg gcggttcaat gaccgggcgc ccaggatcag cccgaacaca
     7501 atgatcgacc cgacgatgcc cacgcccacc cggaccggca tcgcgtcaag gtctggcagc
     7561 gatgccgatg cctgtgccgc agcaggattg ggcgctgcgt ccggcttggg cgcgagcaac
     7621 gacggatccg gctcgatcag cgtgccgacc ttggtgcccg gcgccgtcgc gaaaccatag
     7681 tcgagcaggt gcgccgcttg ctcccatggc gcaataggct cgcgggtgcc gtgcagcagc
     7741 accgccacca accggcgccc gtcgtgattg gcggctccga caaaggtctg acccgcgtca
     7801 tcggtgtagc cggtcttgcc gcccatcgcg ccggggtagt tgacgagcag cttgttgtcg
     7861 ttctcgatgg gaaagccgac atcgccgcgg ccgggaaagt cgaacgtgcg ggtgcccacg
     7921 atgtcggcga aagtcgggtt ctgccaggcg tatcggtaga acaggccgat gtcgtaggcc
     7981 gacgtgctca tgccaggtcc gtcgaggccc gacggcgtcg cggcgcgggt gtcgcggccg
     8041 ccgagcttgc cggccaggtc gttgatcttc tgtatcgcgg tgtccatccc gccgagctgg
     8101 acggccaacg catgcgcggc gtcgttgccg gaatacataa gtaggccatg cagcaagtcg
     8161 ttgatggaat agaagccatt ctcgccgacc ccgaccttgg tgccctcggc gttggcgtcg
     8221 tcttgagtgc ccgccaccac cttgtggatc ggcagttcct tgatcgactg catggcgatc
     8281 agcaccttga tgatgctggc cggtcgatga cggccgtgcg ggtcccgtgc ggcgacgatg
     8341 tcaccggagt ctaggtcggc gacgagccac gcctcggcgg agatgtcccc cggcaccggg
     8401 ggcatgcctg gggcggtgat gatgccgcag cccgacaacg cgtcaccgcc gagtggcttg
     8461 gcaggcaccg gcagcggtgc gggtggatca ccggcctccg gcacctcgga ggagtcgacc
     8521 gcaggcggcg tcgactcccg atacgggcag gccggagcct cgggcgccgc gttcggctcg
     8581 gccgttgcca ccgccggagc cgccaacagg aaaacagccg ctgccag
//
LOCUS       DASRJA010000239         9181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_912244,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000239 DASRJA010000000
VERSION     DASRJA010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9181
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912244"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VFC01_11005"
     CDS             <1..610
                     /locus_tag="VFC01_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HZN80200.1"
                     /translation="DDPREKLRKVLVHHAVTLRTAAILGDEDAALKVLDEHRLLCAHR
                     HGPYGVQHWNRQVERWLTEETGDPIWSAWYAGRPVLVTANDYGLSVYNGDTGVTVARD
                     GALRAVIGSAGETLTFATSRLADVETMHAMTIHKSQGSQADEVTVLLPQEDSRLLTRE
                     LFYTAVTRAKERVRVVGSTAALRAAIERRAVRASGLAQRLRA"
     gene            complement(619..2118)
                     /locus_tag="VFC01_11010"
     CDS             complement(619..2118)
                     /locus_tag="VFC01_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HZN80201.1"
                     /translation="MVTRLSAADAMALRTQTSTPPAHTVVVIILEACDHLSHARLHRL
                     VASSLPELARFRSRLVSKPLGMGQPVWAEIDDYDATRNIQRASVAAPGGCRELADLVT
                     LLSAEPQDWRRSLWKAWTIDGLAGGRWALAVKMSPVLTDGGYGVASLWKHMLTSGPND
                     SADTLRMETSLGSVPSLGELVTDTVSEIIENHIVGVRLVAEAVTGVMQSLLRRPRETP
                     GPPVDSHATSSMSGPVPHTVFNAPLTKRRSMAFASIPLTDVKTVRNAFGGSTANVFLA
                     ACAMSMRAWMQRYDVIPDDPLVMNVPLSLPAGDAAEAGKSLTTGQVRVPVQLADPVQV
                     LTNLYTAAERLNIAHDFCHEKTDRTTDPADALSLLPPLIAQAGMRVCTELGLSRRRAP
                     SYDGSVSFTSGGPARAYCAGAEVIGMHIAEPLVDGCGLHISVTSHADVMDLCLCACPD
                     NVPRVDEIATGIVEAVNTLLVAAAQSPRGKGRSVVTELTSHATRHSHSG"
     gene            complement(2271..2699)
                     /locus_tag="VFC01_11015"
     CDS             complement(2271..2699)
                     /locus_tag="VFC01_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5994 family protein"
                     /protein_id="HZN80202.1"
                     /translation="MALREKRAISNLRRSPRLRLKRKAPMTGYVDGAWWPRGDDLPTE
                     LPDLLAVLSERLGAVARVTYNLAEWAKAPRRVPIGGRIVRLDGYHRQPHNTLGVLDAY
                     GNNVVLLVVPVHTEPDRAHTIVMAAAVTDNASSVDTLLSN"
     gene            3409..5043
                     /locus_tag="VFC01_11020"
     CDS             3409..5043
                     /locus_tag="VFC01_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HZN80203.1"
                     /translation="MSQFTETMYFNARCSLKGMVTGEPGEPVRHTWADVHQRARHIAG
                     GLANAGVGHGDAVAVLAGAPVEIAPTAQGIWMRGASLTMLHQPTPRTDLLRWAEETTA
                     VIDMIEAAAVVVSDPFMPAAPLLAERGIRVLIVQDLLGGRSIDPVDTDDDDIALMQLT
                     SGSTGSPKAVQITHRNFVANAEAMIVGCNFDVDTDVIVSWLPCFHDMGMTGYLTVPMY
                     YGGELVKITPMDFLRDTLLWARLIDKYKGTMTAAPNFAYNLFAKRLRRQAMPGEFDLS
                     SLRWALSGAEQVDPLDVEDLCDAGKPFGLRPEAIIPAYGMAETTVAVSFSECDGGMVV
                     DEVDADLLAILHRAVPANKGHTRRLVSLGRPLDGLELRVIDEDGSVLPPRGVGVIEVR
                     GEPVTRGYTTAAGFLPAQDELGWYDTGDLGYLTETGNVVVCGRLKDVIIMAGRNIYPT
                     DIERAASRVAGVRPGCAVAVRLEAGHSRETFAVAVESKEYGDPAEVRRIQHQVAHEVF
                     AEVDVRPRNVVVLQPGMIPKTPSGKLRRAHAMALVN"
     gene            5082..5885
                     /locus_tag="VFC01_11025"
     CDS             5082..5885
                     /locus_tag="VFC01_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HZN80204.1"
                     /translation="MADYDAQPGRPNPAADLPRAQVEADEIDLYAGLKGVAGLVAGAR
                     GMIDLLGDVAAFAAQAIPGVDGVGVALVQPHDHAPRVQTWAATAVFVHEIENVQFEEL
                     HEGPCITCMESRRPCVSGSLGSDRRWPHFAGRVARMAVHSALSLPLTVGDEVIGSINA
                     YARSRDAFGEHAVQLGAQFAGPAAVSVYNAQLLAATQERTKALQRALDSRAVIDQAIG
                     IIRSRSGVSAEVAFDRLTRLSQAENIKLYLVAERMVEEAVRRARARHSP"
     gene            complement(5882..6637)
                     /locus_tag="VFC01_11030"
     CDS             complement(5882..6637)
                     /locus_tag="VFC01_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HZN80205.1"
                     /translation="MTEIPREARVLDAVVSLVDRLLDDFDVVDLLTDLTERCARLLDV
                     ESAGLLLADPLEQLRVLAATTEETRNLELFQLQSDEGPCVDCYFSGEPVSVADLNAEA
                     DRWPRFVPAALDAGFASVHAVPMRAAGIVLGALGLFGARPGALTDADLLVGQTLAHIA
                     SVAILQEHPPTPSTVMQQLRNALTNRVHVEQAKGFLRERLDISVEEAFQLLRSYAHTH
                     GDHLTDVARQLMTDRQARPPLLAAIAEFDSARP"
     gene            complement(6672..7379)
                     /locus_tag="VFC01_11035"
     CDS             complement(6672..7379)
                     /locus_tag="VFC01_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HZN80206.1"
                     /translation="MAIHDQLLAAVDGRRGVDAADRLCEACVMLFDIDAAAISLVFDG
                     ANSGTLGSSGAPARMYDELQFTLGEGPCLDSITLRTPILVADLADTIDARWPIYGPAM
                     LSHQIRGVFAMPVLVAGEHVGALDFFRAQPGDLAGDHLAGAIGAAELASAPLLDLMND
                     DLLAAVNDPESNAWAELHALSRAEVSQATGMLVAQLDVEPAEALVRLRAHAYATGRSS
                     TDVARDILDRRLTLDTD"
     gene            7722..8195
                     /locus_tag="VFC01_11040"
     CDS             7722..8195
                     /locus_tag="VFC01_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80207.1"
                     /translation="MNEPSDPPAGHHGGQYLRGRTADRRRNRAARTREEFLQQLPARL
                     LIDRLSTPMLAIGLDGVVVYATAACQQLLGYRNGTRLTGQSLPGLLVGHSDTTPEDCI
                     TLLRVPKTIVKWQHTDGYPVSTVVSSTILLRATDPLMMVSLTDVTEWTWAASPRD"
     gene            complement(8243..>9181)
                     /locus_tag="VFC01_11045"
     CDS             complement(8243..>9181)
                     /locus_tag="VFC01_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZN80208.1"
                     /translation="KPTTQLGYGRTYTMSITARGPNGMPSRQTSSFTTLTPGNQTEVY
                     LETTAGGLLQDGATYGVGMVVVAKFDEPIDNKANAERHLVVTTNPPVPGSWDWLDDQT
                     AHWRPEKYYAPGTSVTVAANLYGTPLGDGLYGQEDEHLSFKIGDSHVSIADDTTKQVS
                     VFDNGKLVRTMPTSMGMGGTETIGGTTLSFWTPPGVYTVMDKANPVIMDSSTFGLPIN
                     SRLGYRETIPYATRISSDGIYLHQLNATVWAQGNTDTSHGCLNLNGENAEWFYGFSVP
                     GDVVEVRNTGGPPLQLKQNGDWSVPWNEWRNGSALS"
BASE COUNT         1542 a         2817 c         3228 g         1594 t
ORIGIN      
        1 ggacgatccg cgcgagaagt tacgcaaagt gcttgtgcac cacgctgtta cgttgcggac
       61 ggcggcgatc ctaggtgatg aggacgctgc gctcaaggtg ctggacgagc atcggttgct
      121 gtgcgcgcac cgacacgggc cgtacggagt gcagcactgg aaccggcagg tcgagcggtg
      181 gctgacggaa gagaccggcg acccgatctg gtcggcgtgg tacgcgggcc gaccggtgct
      241 ggtgacggcc aacgactacg ggctgagcgt gtacaacggc gacacgggtg tgacggtcgc
      301 gcgggacggc gcgctgagag cggtcattgg aagcgcaggc gagacgctga cgttcgcgac
      361 gagccggctc gcggatgtgg agaccatgca cgcgatgacc atccacaaga gccagggcag
      421 ccaggccgat gaggtgacgg tgctgctgcc ccaagaggat tcgcggctgc tcacgcgaga
      481 gctgttctac acggcggtga cgcgggcgaa ggaaagggtc cgcgtggtgg gatcgacggc
      541 ggccttgcga gctgcgatcg agcgtcgagc ggtacgcgcg tcaggtttgg cgcagcggct
      601 gcgtgcttag cagggccgtc aaccactatg cgagtgcctg gttgcatgtg acgtcaactc
      661 tgtgacgacg gaccggccct tgccgcgggg cgattgcgct gcggccacca agagtgtgtt
      721 gacggcttcg actatccccg ttgcgatctc gtcgacccgg gggacgttgt cgggacaggc
      781 gcacaggcac agatccatca catcggcgtg tgaggtgacc gagatgtgca agccgcagcc
      841 gtccaccagc ggctcagcga tgtgcatacc gatgacctct gcgccggcgc agtacgcacg
      901 tgctggcccg ccggatgtaa acgacacact cccgtcgtaa ctcggagcgc gccgtcgaga
      961 caggcccagt tcggtgcaga ccctcattcc cgcttgcgcg atcaaaggcg ggagcagcga
     1021 gagggcgtcc gcgggatctg ttgtccgatc ggtcttttca tggcagaagt cgtgggctat
     1081 gttcaaccgc tccgcggctg tgtagaggtt ggtgagcacc tgcaccggat cggcgagctg
     1141 gaccggtacg cgaacctggc ccgttgtcaa cgattttccc gcctcggccg catcgccagc
     1201 tggtaacgac aacgggacat tcatcaccaa aggatcgtcg gggatcacgt cgtaacgctg
     1261 catccaggca cgcattgaca tagcgcaggc ggcgaggaaa acattggctg tactaccccc
     1321 gaacgcgttc ctgaccgtct tcacgtcggt caacgggatc gaggcgaacg ccatcgaccg
     1381 ccgcttggtc agtggtgcgt tgaacacggt gtgcggcacc ggtccgctca tcgacgaagt
     1441 ggcgtgcgaa tccaccggcg gccccggcgt ttcgcgcggt cgacggagca acgattgcat
     1501 cacgcccgtc acggcttcgg ccaccagccg aacgcctacg atgtggttct ctatgatctc
     1561 gctcactgtg tccgtgacga gctcgcccag tgagggcaca gacccgaggc tggtctccat
     1621 ccgcagcgtg tctgccgagt cgttcggccc gctggtcagc atgtgcttcc acaaagaggc
     1681 gacgccgtag cctccgtcgg tcagtaccgg cgacattttc accgccagcg cccagcggcc
     1741 tcccgccaga ccatcgatgg tccacgcttt ccacaacgat cgtcgccaat cctgtggttc
     1801 agcgctcagt agcgtgacca gatcggccaa ctcgcgacat cccccaggcg cggccaccga
     1861 cgcgcgctgg atgtttcgcg tggcgtcgta gtcgtcgatc tcggcccaga ccggctggcc
     1921 catgcccagc ggcttgctca ccagtcggct gcggaagcgc gccaactctg gcagcgacga
     1981 ggcgacaagc cgatgtagtc gggcatggct gaggtgatcg catgcctcga ggatgatcac
     2041 gacgaccgta tgcgcaggcg gagtcgaggt ttgagtgcgc agtgccatcg cgtcagcggc
     2101 ggagagccgg gtgaccatta gctactcggg tcttgagatc gatgcttggg catccatgtc
     2161 gtcatggcca aagtcgttga acgacttgaa agctggttcg ctattgggca tttggggttc
     2221 cctacttttc ggtcgtgaag tgtggggttg ctgtcgagga agccacgcgc tcagtttgag
     2281 agcagcgtgt cgacactgga cgcgttgtcg gtgaccgcgg cggccatcac gatggtgtgt
     2341 gcacggtcgg gttcggtgtg caccggcacg accagcaaga cgacattgtt gccgtaagcg
     2401 tcgagaactc ccagcgtgtt gtgcggctgg cggtggtagc cgtcgagtct gacgattcgc
     2461 ccgccaatcg gaacccgccg tggcgccttc gcccactcgg ccaggttgta cgtcacgcga
     2521 gcaacggcgc cgaggcgttc cgagagcacc gcgagcagat caggaagctc tgtcggtagg
     2581 tcatcgccgc gcggccacca tgccccgtcg acatacccag tcatgggtgc tttgcgcttc
     2641 aatcgcagcc gcggtgaccg acggaggttg gaaatggcac gtttttctcg aagtgccatg
     2701 ggcacaatcc cgtctgagcc tcgccggggg cccaattcac tcgaatcggc gatgacacaa
     2761 tctcagatgg tcgcgtgcga tataccgccg atgtgaccca ctgtacaccc gcaggctcgc
     2821 gcagcggagg aggctgccgg cggcgcggaa agttgttgag ccgtttgtgg attcgaaacg
     2881 tcggaccgag attgaaggcg ctccaacgct aagtcgtgtg gtaaccgctg ccggcgctgt
     2941 atctgcttga cgagcagcag acttccccgt ggcgaacccg ccgggcgtgt tgcaatcgag
     3001 cccaacgtcc ggccgacgcg gtatggtcgt accgggttgg ctcccacagc cccacggaaa
     3061 cgtcacggcg gcgatccgaa ttccccgccg agcgggtgtg aactccgacg tcagccgatg
     3121 tttcacggtt gtgcatacgg ggtggagttt cgtgccaact gaagcaattc caatagcgat
     3181 cggccacggc gcgctgccgt ggtcggaccc gcaagagcct gtacagtcgg agcgtctgac
     3241 acgctcggct cctcgctcca cccggagcgc cggatcatct ggggtgaata cgagaggctc
     3301 cgctgcaccc accgtctgcc ccggcagggg caggctgtcg tacctcagcg gctccaccag
     3361 tgcacaacac attcgcggcg ccatcacgcc ggagccaaag ggattgtcgt gagccagttt
     3421 accgagacga tgtatttcaa cgcccggtgc agcttgaaag gcatggtcac cggcgaaccc
     3481 ggcgaaccgg ttaggcacac ctgggccgac gtgcaccagc gggcccgtca cattgcaggt
     3541 ggactggcaa acgccggcgt cggacatggc gacgcggtgg cggtgctggc cggcgccccg
     3601 gtcgagatcg ccccgactgc gcaaggcatt tggatgcgcg gcgcgagtct gaccatgctg
     3661 caccaaccca cgccgcgcac cgatctgctg cgctgggccg aggaaaccac cgctgtcatc
     3721 gacatgatcg aggccgctgc ggttgttgtg tcggatccat tcatgcccgc cgctcccctc
     3781 ctggctgagc ggggcattcg ggtgctgatc gtccaagacc tgctgggcgg tcgatcgatc
     3841 gatcccgtcg acaccgatga cgacgacatc gcgctcatgc aactgacgtc cggatcgacc
     3901 ggttcgccca aggctgttca gatcacccac cgcaacttcg tcgccaacgc cgaagcgatg
     3961 atcgtcggct gcaacttcga cgtcgacacc gacgtcatcg tgagttggct gccgtgcttc
     4021 cacgacatgg gcatgacggg ctatctcacc gtgccgatgt actacggcgg cgagctcgtc
     4081 aagatcactc cgatggactt cctacgcgat acgttgttgt gggccaggtt gattgacaaa
     4141 tacaagggca ccatgacggc tgcgccaaac tttgcctaca acttgttcgc caagcgattg
     4201 cgcaggcagg ccatgcccgg cgagttcgac ttgtcgtcgc tgcggtgggc gttgtctggc
     4261 gccgaacagg tcgaccccct cgacgtcgag gatctgtgcg acgccggcaa gccgttcggg
     4321 ctgcggcccg aagcgatcat tccggcctac ggcatggccg agacgacggt cgcggtgtcg
     4381 ttctccgagt gcgacggcgg catggtcgtc gacgaggtgg acgctgacct gctggcaatc
     4441 ctgcaccgcg ccgttccggc gaacaaagga cacacccggc ggctggtatc gctgggccgg
     4501 ccgctggacg gcctggagtt gcgcgtcatc gacgaggacg gcagtgtgct gccgccgcgc
     4561 ggcgtcggcg tcatcgaggt gcgcggtgaa cctgtgacaa ggggctacac cacagcggcc
     4621 gggttcctcc ccgcgcagga cgagctgggc tggtacgaca ccggcgacct gggctatctc
     4681 accgaaaccg gcaacgtggt ggtgtgcggc cggctcaagg acgtgatcat catggccggc
     4741 cgcaacatct acccgacaga catcgagcgc gccgcctccc gcgtcgcggg ggtacggccg
     4801 ggctgtgcgg tggcggtgcg gctggaggcc gggcactcgc gcgagacgtt cgccgtggcg
     4861 gtggaatcca aggagtatgg ggacccggcc gaggtgcgcc gcatccagca tcaggtcgca
     4921 cacgaggtgt tcgccgaggt cgatgtgcgg ccgcgcaatg tggtggtgct ccagccgggc
     4981 atgatcccca agacgccgtc ggggaagctg cggcgcgccc acgccatggc gctggtgaat
     5041 tgacgtgatc acatcgtgtg gcgagtaact tatcgctcag gatggccgat tacgacgcac
     5101 agcccggtcg cccgaacccg gcggccgacc tgccccgcgc gcaagtggag gccgacgaga
     5161 tcgacctgta cgccgggctg aaaggggtgg ccgggctggt ggccggcgcg cgcgggatga
     5221 tcgatctgct cggcgacgtg gcagcgttcg cggcgcaagc catccccggc gtcgacggtg
     5281 tcggcgtcgc ccttgtccag ccgcacgacc acgcgccacg cgttcaaaca tgggctgcca
     5341 cagcggtatt cgtgcacgag atcgagaacg tccagtttga ggagcttcat gaggggccgt
     5401 gcatcacgtg catggagtcg cgccggccgt gcgtcagtgg atcgctgggc agtgaccggc
     5461 ggtggccgca cttcgccggc cgggtggcgc gcatggcggt tcattcggcg ctgtcactgc
     5521 cgctgacggt gggcgatgag gtgatcggct cgatcaacgc ctatgcccgc agccgcgacg
     5581 cgttcggcga gcacgccgtg cagttgggtg cacagttcgc cgggcccgcg gcggtgtccg
     5641 tgtacaacgc gcagctcctg gcggcgacgc aggagcggac gaaagcgctg cagcgtgctc
     5701 tcgacagtcg ggcggtgatc gatcaggcga tcggaatcat tcgcagccgg tcgggcgtca
     5761 gcgcggaggt ggcgttcgat cggctcaccc ggctgagcca ggcggagaac atcaagctgt
     5821 acctcgtcgc cgagcgcatg gtggaggaag cggtgcggcg ggcacgagcg cggcatagcc
     5881 cctagggccg ggcagagtcg aattcggcga tggccgcgag cagcggcggg cgggcctgcc
     5941 gatcggtcat gagctgccgg gccacgtcgg tcaaatggtc gccatgggtg tgcgcgtagc
     6001 tgcgcagtag ctggaaagcc tcctcgacgg aaatgtcgag gcgctcccgc aggaagcctt
     6061 tggcctgttc gacgtggacg cggttggtca gcgcgttgcg cagctgctgc atcacggtcg
     6121 agggcgtcgg cgggtgttcc tgcagtatcg ctacggacgc gatgtgggcg agagtttggc
     6181 cgacgaggag atcggcgtcg gtgagtgcgc cgggccgggc gccgaacaag ccgagcgcgc
     6241 caaggacgat gccggcggcg cgcatgggca cggcgtgtac ggaggcgaag ccggcgtcga
     6301 gcgcggcagg gacgaaccgc ggccagcggt cggcttcggc gttgaggtcg gcgacggaga
     6361 ccggctcgcc gctgaaatag cagtccacac aaggaccttc gtcggactgc agttggaaga
     6421 gctccaagtt gcgggtttcc tcggtggtgg ccgcgagcac acggagctgc tcgagcggat
     6481 cggcgagcaa gagtcctgcc gattcgacgt cgagcagccg ggcgcagcgc tcggtgaggt
     6541 cggtcagcag atccacgacg tcgaagtcgt cgagcagcct gtcgacgagc gatacgacgg
     6601 cgtccagtac acgggcttca cgagggattt cggtcacctc gctctccttc cttggttgcg
     6661 aaaggcctgc atcaatcggt gtccagagtc aggcgtcggt cgaggatgtc gcgggcgacg
     6721 tcggtcgagc tgcggccggt ggcgtaggca tgggcgcgga gacgcaccag ggcctcggcg
     6781 ggttcgacgt cgagttgggc gaccagcatg ccggtggcct ggctgacctc ggcgcgggat
     6841 aacgcgtgaa gttcggccca ggcgttgctc tccgggtcgt tgacggcggc taggaggtca
     6901 tcgttcatga gatcgagcaa gggggcgctg gcgagttcgg cggcgccgat ggcgccggcg
     6961 agatggtccc ctgcgaggtc gcccggttgg gcgcggaaga agtcgagggc gccgacatgt
     7021 tcaccggcga cgaggactgg catggcgaac acgccgcgga tctggtggga gagcatggcg
     7081 gggccgtaga tgggccagcg ggcgtcgatg gtgtcggcaa ggtcggcgac gaggatcgga
     7141 gtcctgagcg tgatggaatc caggcagggg ccctcgccga gggtgaactg gagctcgtcg
     7201 tacatgcgcg cgggcgcccc gctggatccg agggttccgc tgttggcgcc gtcgaagacg
     7261 agggagatgg ccgccgcgtc gatgtcgaag agcatcacgc acgcctcaca cagccggtcg
     7321 gcggcgtcta cccctcgccg accgtcgaca gcggcgagga gctggtcgtg gatcgccaca
     7381 tctaaccgaa caggggccgg gcgttagcgg tgcccggggg tcgtggtggc gcggtcatac
     7441 ttccagactc ctccagtggg cggggtattg cgagggcggg gtcggcatcg cggcacggtg
     7501 attcgctcgg gaatgtcctg ccaacgcgcg ttgtcaattc ctcacacaga cgtcgattcg
     7561 cgtcctcaca cagacgtcga ttcgcgcttg ggcttgctat cgtacgagat gttggccaaa
     7621 cagccaggtc gggtgaatac agccgctctc agccgttgtc ttccacaagc gttagctcgc
     7681 gccgaccgat tcggtgacat ccgctcgtcg ggggatcacc agtgaacgaa cctagcgatc
     7741 caccagccgg gcatcatggc gggcagtacc ttcgcggccg aacggcggac cggcgacgca
     7801 atcgcgcggc ccgaacacgc gaggaattcc tacaacagct tccggcccgg ctattgatcg
     7861 accggctgtc cacgccgatg ctcgcgatcg gactcgacgg cgtcgtcgtg tacgccaccg
     7921 cggcctgcca gcagctgctc ggctaccgga acgggacgcg cctcacgggg cagtcgctgc
     7981 ccggactcct ggtggggcat tccgacacga cgcccgaaga ctgcatcacg ctgctgcgcg
     8041 tcccgaagac gatcgtgaaa tggcagcaca ccgacggcta ccccgtctcc accgtcgtgt
     8101 ccagcacgat ccttctccgc gcaaccgacc cgctgatgat ggtcagcctc actgacgtca
     8161 ctgagtggac gtgggccgcg agccctcgcg actaggcctc gacgagccga ggcgggcttc
     8221 cgctggcgag gcgtcgatca ggtcaggaca gggcgctgcc atttcgccac tcgttccacg
     8281 gcacgctcca gtcgccgttc tgcttcagct gcaacggcgg gccgccggtg ttgcgcacct
     8341 cgacgacgtc gccgggcacg gagaaaccgt agaaccattc ggcgttctcg ccgttgaggt
     8401 taaggcagcc gtggctcgtg tcggtattgc cttgggccca caccgtcgcg ttgagctggt
     8461 gcaggtagat gccgtcgctg ctgatgcggg tggcgtaggg aatggtctcg cggtacccga
     8521 ggcgggagtt gatcggcagc ccgaaggtcg aggagtccat gatcaccggg ttggccttgt
     8581 ccatcacggt gtagacaccc ggcggggtcc agaagctgag tgtggtgcca ccgatggttt
     8641 cggtgccgcc catgcccatc gaggtcggca tggtgcggac cagcttgccg ttgtcgaaga
     8701 cgctgacttg tttggtggtg tcgtcggcga tggagacgtg cgagtcgccg atcttgaacg
     8761 acaggtgctc gtcctcttgg ccgtagagcc cgtcgccaag cggcgtgccg tagaggttgg
     8821 cggcgacggt gacggatgtg cctggtgcgt aatacttttc gggccgccag tgcgcggtct
     8881 ggtcgtcgag ccagtcccac gagccgggaa ccggtgggtt ggtggtgacg acgaggtgac
     8941 gttcggcgtt ggccttgttg tcgatgggtt cgtcgaactt ggcgacgacc accatgccga
     9001 cgccgtatgt cgcgccgtcc tgcagtaggc ctccggcggt cgtttcgaga tagacctcgg
     9061 tctggttgcc gggggtgagg gtggtgaagc tggacgtctg ccgggacggc atgccgttgg
     9121 gcccgcgagc ggtaatgctc atggtgtagg tgcggccgta gccgagttga gtcgtcggct
     9181 t
//
LOCUS       DASRJA010000240         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3989628,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000240 DASRJA010000000
VERSION     DASRJA010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3989628"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /gene="preA"
                     /locus_tag="VFC01_11050"
     CDS             complement(<1..948)
                     /gene="preA"
                     /locus_tag="VFC01_11050"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015649952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HZN80209.1"
                     /translation="MADLTTNFLGIKSPNPFWLASAPPTDKAYNVQRAFEAGWGGAVW
                     KTLGEDPPIVNVSGPRYGAMHGNDRRVLGFNNIELITDRPLGVNLEEVRQIKRDWPDR
                     ALIVSIMLPMHEESWAKYVPMIEDTGADGLELNFGCPHGMSERGMGSAVGQVPEYIQL
                     AAEWCKKYARVPVIVKLTPNITSILPAARAARAGGADAVSLINTLNSIMRVDYDTLTI
                     YPATDGKGSHGGYCGPAVKPIALHMVAEIARSRETAGLPISGIGGITDWRDAVDFLAL
                     GATTVQVCTAAMVYGFKIIDDLVDGLDNFLDRKGFASVRE"
     gene            complement(980..2350)
                     /locus_tag="VFC01_11055"
     CDS             complement(980..2350)
                     /locus_tag="VFC01_11055"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80210.1"
                     /translation="MSSNKPVADPADVRPGRLADEQIAANFADMHPPLTPSEAIIDAD
                     RCYFCYAAPCTTACPTGIDIPGFIQRIRSGNLKGSAYTILSENIMGGMCARVCPTEVL
                     CEQACVRNTHEDRPVRIGLLQRYATDPVFDEAIPLFERAPATGCTVAVVGGGPAGLSC
                     AHRLAMLGHSATVFEPKEKLGGLNEYGIAAYKTPDDFAAREVEYILSVGGIDVRTGVA
                     LGKDFSLGELRTQYDAVFLGFGLAGVNDLGLDAEQVEGVANAVDYIATLRQVANKASL
                     PVARRIVVIGGGMTAIDIAVQSKALGAEEVTIAYRRGQESMNASRYEQEFAQVRGVAV
                     RYWSSPRRLLTRDGRVRAVEFETTRSANDGRLEGTGQTFVLDADVVFKAIGQKVLWER
                     VGGTAELVDVNQDRIVVDEERKTSLPGVWAGGDCVHGGADLTVSAVQDGKLAALSIDR
                     SLRQAK"
     gene            complement(2390..>2806)
                     /locus_tag="VFC01_11060"
     CDS             complement(2390..>2806)
                     /locus_tag="VFC01_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN80211.1"
                     /translation="IENRLEVLWHHGVNTGRLTMNEFVRVTSTNAAQIFNIYPRKGSV
                     SLGSDADLVVWDPAASKTISAKTHRQNVDVNIFEGMTVQGCASHTVSGGKLVYANGDL
                     RVERGAGRYVKRPAFAPYYDAVRKQLELSAPRPVRR"
BASE COUNT          464 a          942 c          876 g          524 t
ORIGIN      
        1 ctcgcgtacc gaggcaaagc ccttgcgatc gagaaaattg tcgagcccat ccaccaggtc
       61 atcgatgatc ttgaacccat agaccatcgc cgccgtgcag acctggacgg tcgttgcacc
      121 cagggcgagg aagtcgaccg cgtcgcgcca gtcggtgatc ccgccgatac cggagatcgg
      181 cagtcccgcc gtctcccgcg agcgggcgat ttccgcaacc atgtgcaggg caatcggctt
      241 gacggccgga ccgcagtagc cgccgtgcga gcccttgccg tcggtcgccg ggtagatggt
      301 gagggtgtcg tagtcgacgc gcatgatcga gttcagcgta ttgatcagcg acacggcgtc
      361 ggcgccgcca gccctggcgg ccctggccgc gggcaggatg ctggtgatgt tgggcgtcag
      421 cttgacgatc accggcacgc gcgcgtactt cttgcaccac tcggcagcca gctggatgta
      481 ctcgggcacc tggcccacgg ccgagcccat gccgcgctca gacatgccgt gaggacagcc
      541 gaagttgagc tcgaggccgt ccgcgccggt gtcttcgatc atcggcacgt acttcgccca
      601 gctctcttcg tgcattggca gcatgatcga aacgatcaag gcccgatcgg gccagtcgcg
      661 cttgatctgg cggacctcct cgaggttgac tcccaatggc cggtcagtga tcagctcgat
      721 gttgttgaaa ccaagcacgc gccggtcatt gccatgcatg gcgccgtaac gcggaccact
      781 gacgttgacg atcggtggat cctcgccgag ggttttccag acggcaccgc cccagcctgc
      841 ttcgaaagcg cgctggacgt tgtaggcctt gtcggtcggc ggggccgatg ccagccagaa
      901 ggggttcggt gacttgatgc cgagaaaatt ggtggtcaga tcggccattt cattaacctc
      961 gcctcagtgc agccgcaggt catttcgcct gcctgaggga tctgtcgatg gatagggcgg
     1021 cgagttttcc gtcctgcacg gcagacacag tcagatctgc gccgccatgt acacagtcgc
     1081 cgccggccca cacacccggt agtgaggtct tcctttcttc gtcgactacg attctgtcct
     1141 ggttcacgtc gacgagctcg gctgtcccgc cgacccgctc ccagagcacc ttctgtccga
     1201 tcgccttgaa cacgacgtcc gcgtcgagca cgaaggtctg cccggttcct tccagacggc
     1261 cgtcgttcgc gctccgcgtt gtctcgaact cgacggcgcg gacgcgtcca tcccgggtca
     1321 gcagcctgcg cgggctcgac cagtaccgga ccgccacgcc acggacctgg gcgaactcct
     1381 gctcgtagcg gctggcgttc atgctttcct ggccgcgccg gtaggcaatg gtcacttcct
     1441 ccgcgcccag ggccttgctt tgaaccgcaa tgtcgatcgc ggtcatgccg ccgccgatca
     1501 cgacgattct gcgggcgacc ggcagcgagg ccttgttcgc cacctgccgc agagttgcga
     1561 tgtagtcgac ggcgttcgcc acgccctcga cctgctcggc atcgaggccc aggtcgttga
     1621 ccccggcgag accgaagccg agaaacaccg cgtcgtattg cgtgcgcagc tcgccgagcg
     1681 agaaatcctt gcccagcgcc acaccggtcc ggacgtcgat gccaccgacg ctcagaatgt
     1741 actcgacctc gcgagccgcg aaatcgtcgg gggtcttgta cgcggcgatg ccgtactcgt
     1801 tcaggccgcc cagcttctcc ttcggttcga acaccgtcgc gctgtgtccg agcatcgcca
     1861 ggcggtgcgc gcaactcagg ccagccgggc cgccgcctac caccgcgacc gtgcacccgg
     1921 ttgccggcgc gcgctcgaag agcggaatcg cctcgtcgaa aaccggatcc gtggcgtagc
     1981 gttgcagcaa gccgatccgt actggccggt cttcgtgcgt gttgcgcacg cacgcctgct
     2041 cgcacagaac ctccgtggga catactcggg cgcacatgcc gcccatgatg ttttcgctca
     2101 ggatcgtgta ggccgagccc ttgaggttgc cgctgcgtat cctctggatg aagccgggta
     2161 tgtcgatgcc ggtcggacag gccgtcgtgc acggggctgc gtagcagaag tagcaacggt
     2221 cggcatcgat gatcgcctcg gacggcgtca gcggcggatg catgtctgca aagttggccg
     2281 ctatctgctc atcagcgaga cgccccggac ggacatcggc aggatcggcg accggcttgt
     2341 tgctgctcac tgaaacctcg cgactgcgct cactcgtaac tctgcgccgt cagcggcgca
     2401 ccggccgcgg cgcgctcaac tccagctgct tcctgaccgc gtcgtagtac ggggcaaacg
     2461 ccggtcgctt gacgtaccgg ccggccccgc gctcgacacg cagatcgcca ttcgcataca
     2521 ccagcttgcc gccactcacg gtatgcgagg cgcagccttg caccgtcatg ccctcgaaga
     2581 tgttgacgtc gacgttctgg cgatgcgtct tcgctgaaat ggtcttgctc gccgcaggat
     2641 cccagaccac caggtcggca tcggagccga gcgatacact gcccttgcgc ggatagatgt
     2701 tgaagatctg ggccgcgttg gtggaggtga cgcgcacgaa ctcgttcatc gtcagtcgcc
     2761 cggtattgac gccgtggtgc cacagcacct ccaggcggtt ctcgat
//
LOCUS       DASRJA010000241         8328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1167156,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000241 DASRJA010000000
VERSION     DASRJA010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167156"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..492
                     /locus_tag="VFC01_11065"
     CDS             223..492
                     /locus_tag="VFC01_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HZN80212.1"
                     /translation="MSYRVGDESSRRVIDVGIGILIGLRGCSDQEAFDELVRVVHQTG
                     VGIGRLAAGLVALASGSSSADHAEAFTVWGDLIRSRRQVSLVTTH"
     gene            complement(763..1209)
                     /locus_tag="VFC01_11070"
     CDS             complement(763..1209)
                     /locus_tag="VFC01_11070"
                     /inference="COORDINATES: protein motif:HMM:NF014552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HZN80213.1"
                     /translation="MRIALVSDEPYPVHSTVRDWLAERGHEVVPFGSVATGEESPWAL
                     AAEQAAEAVARGDCDQGIFFCWTGTGISMAANKVSGIRAALCSDPGQAAGARVWNHAN
                     VLCLSNRTLADDLAKEILQVWFDTEPGERGSAGVADLAGVDERHRR"
     gene            complement(1287..2348)
                     /locus_tag="VFC01_11075"
     CDS             complement(1287..2348)
                     /locus_tag="VFC01_11075"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HZN80214.1"
                     /translation="MRPAASTPNRTVGQLSWTGEKLWEWGTEAPGGAARQNHDWELLE
                     NGNRLILVAIPREVAALGPHVVGDQGVYEVAPDGRIVWEWKAGDHLDEFGFTTEGFEY
                     LRRSVARQPDNIWGYLEINSAKTLGPNRWHREDPDTVFAPDNMLISSRKANIVALIDK
                     RTGKIVWRLGPYFDAEPGSEHQRINSHSLPRPLDQISGQHNPHFIADGLPGAGNLLVF
                     DNQGGAGYPPAPLGIYAGSRVLEIDPVTKHIVWQYTAEDSGRPPWTFFSSFVSNAQRL
                     PNGNTLITEGMHGRIFQVTPSGEVVWAYLTPYEDYAVAGEPEVKVPTVAGVDRPTLTR
                     MIYRSQAVPFDWVPADTPQ"
     gene            complement(2603..2734)
                     /locus_tag="VFC01_11080"
     CDS             complement(2603..2734)
                     /locus_tag="VFC01_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80215.1"
                     /translation="MVEDVYLTAPDGPMVAGVRLDGIWVWIVVALGAAIVAFGGLAL"
     gene            2997..3359
                     /locus_tag="VFC01_11085"
     CDS             2997..3359
                     /locus_tag="VFC01_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HZN80216.1"
                     /translation="MGDDDGVPEVEPDYRFTLANERTYLAWERTALGLLAAAVAVVQL
                     VPELSIPGARHVLGIILALLAILTACMGILRWEQIDRAMRRDLPLPRHPKPVYLGIGL
                     VVVGLLTVGFVVIKAVVG"
     gene            3356..3673
                     /locus_tag="VFC01_11090"
     CDS             3356..3673
                     /locus_tag="VFC01_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HZN80217.1"
                     /translation="MTTPGRRETQAERTTLAWTRTSLGVLANGALLLIKDAHRDASAF
                     RFVAAGFAALLALSTYLLGVRRQRLLAKRPLPEGISPRQEVYLVGASIIFLILISVLS
                     VTV"
     gene            complement(3678..4388)
                     /locus_tag="VFC01_11095"
     CDS             complement(3678..4388)
                     /locus_tag="VFC01_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HZN80218.1"
                     /translation="MFLGTATRACAVGAVIAASVLLSGCVSTVSGTAVRAQHGAPIDV
                     PTLTEAKLDDVLLSVGELNGIMGSSQMKVTSELEEMTDHSGEVSDPDCLGAIYGAEEP
                     VYAGSGWTAVRDQVVREPGEDNEHWVEQTAVLYPSAEKAQKFFDESKSTWEKCSGYSV
                     SVDDVDATYLWQIDDVTSDDSVITQMTAQEDADGWACQHALSVVSNLTVEAWACSYNI
                     KDEAATIANDMVANASKK"
     gene            complement(4465..5187)
                     /locus_tag="VFC01_11100"
     CDS             complement(4465..5187)
                     /locus_tag="VFC01_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HZN80219.1"
                     /translation="MRSTRSTIAALSACAVLAGCSSTPVDGAPVVHIAEAAKPSPTRA
                     LEQVLPTVDELATTFGTAGFMGQLVKGGPDMLLQGVRESEATPIDCVSAGYRLEKIVY
                     QGSPVQSVASRSWIGGDQNGPSYAGFFGAVQFDNATDAQAFFAASADKWHRCNGQTLV
                     LHQPERGADGSSRITDVVVDPKVVSAVVLHDDGTTIQRALAVASDCIVDVEVTNPPAN
                     SGSGAAGAVGIANLMLQKVAAS"
     gene            complement(5195..7107)
                     /gene="lepA"
                     /locus_tag="VFC01_11105"
                     /pseudo
     CDS             complement(5195..7107)
                     /gene="lepA"
                     /locus_tag="VFC01_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216920.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
     gene            7126..7728
                     /locus_tag="VFC01_11110"
     CDS             7126..7728
                     /locus_tag="VFC01_11110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZN80220.1"
                     /translation="MLGNMAPPWKTFQRFAENLVFNEAPKFIRNIQRSETLQRGLQTS
                     IRIGIDALTGAPDDEAPAIAAGRPVTQNSVPTAHRARKLVYAPDLDGRADPGEIVWTW
                     VVYEDDPTKGKDRPVLVVGRDRSTLLGLMLSSQDHHRDDPDWVGIGSGSWDYEGRASW
                     VRLDRVLDVPEEGIRREGAILERETFEVVAARLRAEYSWS"
     gene            complement(7725..>8328)
                     /locus_tag="VFC01_11115"
     CDS             complement(7725..>8328)
                     /locus_tag="VFC01_11115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN80221.1"
                     /translation="SVDTRPLHRRDVRAAAGVLGRAFYDDPVTMWMVPDNESRARALP
                     RGFATIARRHFLSRGGSEVASRDGVIGGATLWDPPGRRKDSRLEKLSMLPGFLWAFGR
                     RTQQMEQVVSLMEENHPEEPHWYLMLIGTDPTVRGTGFGQALMHSRLDRCDAEHAPAY
                     LEASKPDLVPYYSRFGFEVTGEIKLPDGGPTMFPMWRAPR"
BASE COUNT         1405 a         2676 c         2655 g         1592 t
ORIGIN      
        1 ggcacttggg caacttcccg caggcgttca cccacctggc gttgatcaac gccgtcgtgc
       61 acgtcatccg ggccgaggaa gaggccgaca gctccggggt gttcgtaccc gccaacgccc
      121 cgatgtaggg acggttgcgc gcggatgacg gctcggtagc gcttcaggat catcgtgttt
      181 cgatgccttg tcgtagcatt ccccgtcacg caccatgcat gaatgagtta tcgcgtggga
      241 gatgagtcgt cccgacgggt gatcgatgtg ggcatcggca ttctgatcgg attgcgcggc
      301 tgctcggacc aagaggcgtt cgacgaattg gtccgcgtcg tccatcagac cggtgtcggt
      361 atcggacgac tggccgcggg tttggtggcg ctggccagcg gctcgtcgtc tgcggaccac
      421 gccgaggcct tcaccgtctg gggcgatctg atcaggagcc gccggcaggt atcgctggtc
      481 accacccatt gatggccatc aagctgtgac ttctgtgtgt tcacatggca ggcgacgaac
      541 cagtgccgcc acttcgcaat agttactgat cttgctcggg atcagccacg ctgactcacc
      601 attcctgaat tgaatttcag tgcgccacag ggatttccca ccgtagggtc gaactctgag
      661 tctgttgacc acacggctta agtcgggctc tgacctccgg ctgattgact gctgggctcc
      721 tcaaatggcg gtcgcggcgg atcatgggaa tgcttgccga gattaacggc ggtgtcgttc
      781 atccactcct gctaggtccg caacgccggc gctgcctcgc tcgccgggct cagtgtcgaa
      841 ccagacctgc aggatttcct tcgcgagatc atcagcgagt gttcggttgg acaggcacaa
      901 gacgttggcg tggttccaga cgcgagcgcc cgccgcttgg cctgggtcag aacaaagcgc
      961 tgcccgaatg ccagacacct tgttagccgc catgctgatg cctgtcccgg tccagcagaa
     1021 aaagatgccc tggtcgcagt cgcctcgggc gactgcctcc gcggcctgct ccgcggcgag
     1081 tgcccacggg ctttcttcgc ccgtggcgac ggagccgaac ggcaccacct cgtggccgcg
     1141 ttccgctagc cagtcacgca ccgtcgaatg gacgggatac ggctcgtcgg agacgagtgc
     1201 gatgcgcata cgtcgagtct aggaggccgt gactgcgagt tcggtgcgct tacgaccgct
     1261 cagcgaatgt ttgcgcacca aaatcgctat tggggggtgt ctgcaggcac ccaatcgaaa
     1321 ggcacggcct gcgaccggta aatcatccgc gtcaacgtcg gccgatcaac accggcgacc
     1381 gtcggaactt tgacttctgg ttcgcctgcg actgcatagt cctcgtacgg tgtgaggtac
     1441 gcccaaacca cctccccgga cggggtgacc tggaagatcc ggccgtgcat gccttcggtg
     1501 atcaacgtgt tcccgttggg taggcgctgc gcgttgctga caaatgagct gaagaacgtc
     1561 cacggtgggc ggcccgagtc ctctgcggtg tactgccaca caatgtgttt cgtgacgggg
     1621 tcgatctcga ggacccgcga cccggcgtag atcccaagag gcgcaggcgg gtaacccgca
     1681 ccgccctggt tgtcgaacac cagcaggttg cctgcgcccg gaagcccgtc ggcaatgaaa
     1741 tgtgggttgt gctggccgga gatctggtcg agcggacgtg gcagcgagtg actgttgatc
     1801 cgctggtgct cactgcccgg ctcagcatcg aagtacgggc ccagtcgcca cacgatcttt
     1861 cccgtgcgct tgtcgatcag cgcgacgatg ttggccttgc gcgagctgat cagcatgttg
     1921 tcgggcgcaa acaccgtgtc ggggtcctcg cggtgccacc ggttcggtcc gagcgtcttt
     1981 gcgctgttga tctcgagata gccccagatg ttgtcgggtt gacgcgcgac gcttcgccgc
     2041 aggtactcga atccttcggt ggtgaaaccg aattcatcga ggtggtcacc ggccttccat
     2101 tcccagacga ttctcccgtc cggtgccacc tcgtagacac cctgatcccc gacgacgtgt
     2161 gggcccagcg ccgcaacctc gcgcgggatc gccaccaaga tgaggcggtt gccgttctcg
     2221 agcagttccc agtcgtggtt ctggcgggcc gcaccgccgg gtgcctcggt cccccactcc
     2281 cacagcttct cccccgtcca cgatagctgg ccgacggtgc ggtttggcgt agatgccgcc
     2341 gggcgaatcg tccagctgtg tcagttgcac accgatgtcg ccgatgcgtc cgtcattgag
     2401 ggcggggtcg ataatccgtg ccggcacacc gggatacggc cattcgttgc gcaccacacc
     2461 gttgaggtcg atcagtcgtg tgatgccgtc ggcgccggtg tagagcacgt aaccgcccca
     2521 cgcaagatcg gggtcgtggt acgtgacacc gctgaactga acgaaggcca tggcgagtag
     2581 tcctaccaac gagagttagt cgtcacagcg ctagcccgcc gaaggcgacg atggctgcgc
     2641 ccagagcaac gacgatccac acccagatgc cgtcgagacg cacaccggcc accattgggc
     2701 cgtccggagc cgtgagatag acgtcttcga ccacgtagga aggctaggtg tcagcggccg
     2761 caatcgacgg gcgccaacgc cggtttgccg catgcccgga atacaacatg gccggtagag
     2821 accgtctctc acctttgaat cagagtggtt cgaaacggcg gccgaggaat gactcttctg
     2881 cggcgcgtca tcacatgttg acgacgtcgg gaagcgctgc tgctgggatt catcattgcg
     2941 ttctgggcga cgccattccc ggacttcgac gggcgcgggt tcgataggtt gatcccgtgg
     3001 gcgacgacga cggcgttcct gaggtcgagc ctgattaccg cttcaccttg gcgaacgaac
     3061 gcacatatct ggcgtgggag cgcaccgcgc ttggtctgct cgccgcggcg gtggccgtcg
     3121 tccagttagt gcccgagctg tcgatccccg gcgcccgcca tgttctcgga atcatcctgg
     3181 cgttgctcgc gatcctcacc gcgtgcatgg ggattctgcg gtgggaacag atcgatcgcg
     3241 cgatgcgccg cgacctgccg ttgccgcggc atcccaagcc cgtctatctc ggaatcggcc
     3301 ttgtcgtcgt cgggctgttg accgtcggct tcgtggtgat caaggcggtg gtggggtgac
     3361 gacccctggc cggcgtgaaa cacaagccga acgaaccact ttggcctgga ctcgaacctc
     3421 gctcggcgtc ctggccaacg gtgcgctcct gctgatcaag gacgcccatc gcgatgccag
     3481 cgcattccgg ttcgttgcgg ccgggttcgc cgccctattg gcgttgtcga cctatctgct
     3541 cggcgttcgg cgtcagcgat tgctcgccaa acgtccactt ccagaaggta tttcgccccg
     3601 ccaggaggtc tatctcgtcg gtgcgtcgat catttttctg atcctgatct ccgtgctttc
     3661 cgtcacggta tgacgcgcta ctttttcgaa gcgttggcga ccatgtcgtt ggcgatcgtc
     3721 gccgcttcgt ccttgatgtt gtaactgcac gcccaggcct cgacggtcag gttcgacacc
     3781 accgaaagcg catgctgaca ggcccatccg tcggcgtctt cctgcgcggt catctgggtg
     3841 atcacgctgt cgtcggacgt gacgtcgtcg atctgccaca gataggtggc gtcaacatcg
     3901 tccaccgaaa ccgaatagcc cgagcatttc tcccacgtcg acttcgattc gtcgaagaac
     3961 ttctgtgcct tctcggcgga cgggtagagc accgcggtct gctccaccca gtgttcgttg
     4021 tcctcaccgg gctcgcggac gacctggtca cgaaccgccg tccatccact tccggcatag
     4081 acgggctcct ccgccccgta gatcgcacca aggcagtcgg gatcggacac ctccccagag
     4141 tggtcggtca tctcttccag ctcgctggtg accttcatct gcgacgagcc catgatgccg
     4201 ttgagttcgc caaccgacag caggacgtcg tcgagcttgg cctcggtcag cgtcggcacg
     4261 tcgatcggtg cgccgtgctg ggcgcgcact gcagtgccgg acacggtgct cacgcaaccc
     4321 gagagcagga cggacgccgc gatgacagca ccgacggcgc atgcacgggt agccgtgccg
     4381 agaaagatca ccgcgctatt gtgaccgacc gccgcgcgaa gcgtcggcta gccgtcaatt
     4441 tttgctggta cttgacgatc cggtctacga agcggcaact ttttgcagca tcagattggc
     4501 gatgccgacg gcacccgccg cgccgctgcc actgtttgcg ggggggttcg taacctctac
     4561 gtccacaatg caatccgacg ccacggcgag cgcccgctgg atcgtcgttc cgtcgtcgtg
     4621 caagaccacg gccgacacga ccttcgggtc gacgacgacg tcggtgatcc tgctcgaccc
     4681 gtccgcgccc cgctcgggtt gatgcagcac cagcgtctgt ccattgcagc ggtgccactt
     4741 gtcggccgac gccgcgaaga acgcctgggc gtcagtggcg ttatcaaact ggaccgctcc
     4801 gaaaaagcct gcgtacgaag gaccgttctg atcgccgccg atccacgatc ggctcgccac
     4861 ggactgcacc gggctcccct ggtaaacgat cttctccagc cggtagccgg cactcacaca
     4921 gtcgatgggc gtcgcctcgg attcgcgcac gccctgcagc aacatgtcgg gaccaccctt
     4981 gacgagctga cccatgaagc cggcggtgcc aaaggtggtg gcgagctcgt cgacggtggg
     5041 cagcacctgc tccaacgccc gcgtcggcga cggcttggcg gcctcggcga tatggacgac
     5101 gggcgcgccg tcgactgggg tcgacgagca ccccgccagc accgcacacg cgctcaacgc
     5161 cgcgatcgtc gaccgggttg agcgcatcgg atacctactt cttggccttg tcggccgggg
     5221 catcggtgga cagggcggcg acaaacgcct cctgtggcac ctcgacccga cctatggtct
     5281 tcattcgctt cttgccttct ttttgtttct ccagcagctt tcgcttgcgc gtgatgtcac
     5341 cgccgtaaca cttcgatagc acgtctttgc gaattgcgcg gatgttttcg cgagcaatga
     5401 tcttggaacc gatggccgcc tgcaccggaa cttcgaactg ctggcgcgga atcagctcct
     5461 tgagtttggt ggtcatcttg ttgccgtagg cgtacgccga ctccttgtgc acgatcgcgg
     5521 aaaacgcgtc gacggcctcg ccctgcagca gaatgtcgac cttcaccagc tccgcttcct
     5581 gctcgcccgc ctcctcgtag tcgaggctgg cgtagccgcg ggtgcgcgac ttcagcgcgt
     5641 cgaagaaatc aaagatgatc tcccccaacg gcattgtgta acgcagctcg acgcgctcgg
     5701 gcgagagata atccatgccg ccgagttcgc cacgccgcga ctggcacagt tccatgatgg
     5761 tgccgatgaa ttcgcttggc gcgatgatgg ttgtcttcac cacgggctcg tacaccgtgc
     5821 ggatcttgcc gtcgggccag tccgagggat tggtgacgac gatctcggag ccgtcctcct
     5881 gcacgacccg gtacaccacg ttcggcgagg tggaaatgag gtcgaggtcg aattcacgct
     5941 cgagccgctc gcgggtgatc tccatgtgca gcaggccgag gaagccgcag cggaaaccga
     6001 agccaagtgc gacggaggtt tccggctcat aggtcagcgc ggcgtcgttg agctgcagct
     6061 tgtccagcgc gtcgcgcaga tccggatagt cggagccgtc caccggatac aacccggagt
     6121 agaccatcgg ccttggctcg cggtagcccg taagcggctc ttcggcgcca tggcgcgctg
     6181 ccgtcacagt gtcgccgacc ttggactggc gcacgtcttt cacaccggtg atcaggtaac
     6241 ccacctcgcc gacgccgaga ccggcggacg gtttcggttc cggtgagacg atgccgacct
     6301 cgagaagctc gtgggtggcg ccggtggaca tcatcttgat gcgctcgcgt gggacgatct
     6361 tgccgtcgac gacgcggacg tacgtcacga cgccgcggta ggtgtcgtag accgagtcga
     6421 aaatcatggc gcgcgccggg gcgtcgccgt caccggtggg cggcggcacc tcgcgtacga
     6481 cgtggtcgag caggtccgcg acgccctcgc cggttttgcc ggatacgcgc aacacgtcgc
     6541 tgggttcgca gccgatgatg tgggcgattt cgccggcgta gcggtccggg tcggcggcgg
     6601 gcaggtcgat cttgttgagc accgggatga tgtgcagatc gcggtccagc gccaaatata
     6661 ggttggccag cgtctgcgcc tcgatgccct gggcggcgtc gaccagcagc accgccccct
     6721 cgcaggcctc cagcgcgcgc gacacctcgt aggtgaagtc gacgtggccg ggcgtgtcga
     6781 tcaggtggag cacgtagtcc gtgtcatcga ctttccaagg cagccggacg ttttgggcct
     6841 tgatcgtgat gccgcgctca cgttcgatgt ccatccggtc caggtactgc gcgcgcatcg
     6901 agcgctcgtc gaccacaccg gtgagttgca gcattcgatc cgccagcgtc gacttgccgt
     6961 gatcgatatg ggcgatgatg cagaagttcc gaatctgcgc cggcgcggtg aaggtttggt
     7021 cggcgaaact gctgatggga atctcctggt cggacgggct tgatgtgcct ccagcgtatc
     7081 gaggcggggc cgtctcgaca caatcacgcg cacccgcggt cccctatgct cggaaatatg
     7141 gcgccgccgt ggaagacctt tcaacggttc gcggagaacc tcgtgttcaa cgaggcgccg
     7201 aagttcatcc gcaatattca gaggtctgaa acgctgcagc gcggcctcca gacgagcatc
     7261 aggatcggta tcgacgccct caccggcgcc cccgacgacg aggcccccgc aatcgcggcc
     7321 ggtcgcccgg tcacccagaa cagcgttcca accgcgcacc gagcccgcaa gctggtctac
     7381 gcccccgatc tcgacggccg cgccgatccc ggcgagatcg tgtggacgtg ggtggtctat
     7441 gaggatgatc cgacgaaggg caaggaccgg ccggtgctcg tggtgggcag ggaccgttcc
     7501 acgttgctcg gcctgatgct gtcgagccag gaccatcatc gcgacgatcc ggactgggtc
     7561 ggcatcggca gcggcagctg ggactacgag ggccgagcca gctgggtgcg gctggacagg
     7621 gtgcttgacg tgcctgaaga gggcattcgg cgcgaaggcg cgatcctgga gcgggagact
     7681 ttcgaggttg tcgccgcgcg cctgcgcgcc gagtactcct ggagttagcg cggcgcccgc
     7741 cacatcggaa acatggtcgg tccgccgtcg ggcagcttga tctcgccggt gacctcaaac
     7801 ccgaaccgcg agtagtacgg caccaaatcg ggcttgctcg cctccagata ggcaggcgcg
     7861 tgctcggcgt cgcaccggtc gagacgcgaa tgcatcagtg cctgcccgaa gccggtgccg
     7921 cggaccgtcg ggtcggtgcc gatcagcatc aggtaccaat gcggctcttc gggatggttt
     7981 tcctccatca ggctgacgac ctgttccatc tgctgggttc ggcgaccgaa cgcccacagg
     8041 aagccgggaa gcatcgacag cttctccagc cgcgaatcct tccgtcggcc aggcggatcc
     8101 cacaaggttg cccctccgat gacaccgtcg cgactcgcga cttcggagcc gccgcgggat
     8161 aggaagtggc gtcgcgcgat cgttgcgaag ccccgcggca gcgctctcgc cctggactcg
     8221 ttgtcgggca ccatccacat cgtcaccggg tcgtcgtaga acgcccgacc gagaacgcct
     8281 gccgccgcac gaacgtcgcg ccggtggagc ggacgggtgt cgacggac
//
LOCUS       DASRJA010000242         4959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_624104,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000242 DASRJA010000000
VERSION     DASRJA010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624104"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /gene="nuoN"
                     /locus_tag="VFC01_11120"
     CDS             complement(<1..1260)
                     /gene="nuoN"
                     /locus_tag="VFC01_11120"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HZN80222.1"
                     /translation="MNLPTPSVEYGLLSPMLIVLGVAVVGVLVEAFLPRQQRYAAQLV
                     LSLGGLAAAFVAVVGLTRDLHGGTGHTAVLGAIAVDAPALFLQATILLVGVLGILLIA
                     ERRISAPADAETGPGLDGFTPQASAIVGSVTEKLATKAGVMQTEVFPLTMFAIGGMLI
                     FPAADDLLTMFIALEVLSLPLYLLCGLARRRRLLSQEASVKYFLLGAFSSAFFLYGAA
                     MLYGYAGTLDLGGIADAVSSGSGKTSLALIGTGLVLVGVLFKVGAVPFHSWIPDVYQG
                     APTPITAFMAAATKIAAFGAMLRIFYVAVPGLHTEWRPVMWAIAILTMVVGTVTAVTQ
                     NDVKRMLAYSAVAHTGFILTGVIAQNEAGVSSTLFYLFAYGFSTVGAFAVVGLVRNPD
                     GEEDTALERWAGLGRRYPVVGIVFSLFL"
     gene            complement(1257..2825)
                     /locus_tag="VFC01_11125"
     CDS             complement(1257..2825)
                     /locus_tag="VFC01_11125"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZN80223.1"
                     /translation="MVTSIPWLTILWAIPMAGAALVMVLPASQRMVAKWLALLTSLAV
                     LAVSLVVAVGFKPGGDQYQFVENHRWIPSFGTGYILGVDGIALAIVVLTTVLVPLLII
                     AGWNDASDQIGWAGRSVHTYFALTLAVEGMVLMSLVSLDVLLFYVFFEAMLIPMYFLI
                     GGFGGENRSKAAVKFLLYNLFGGLIMLAAVIGLYVVTAQSDVFAAGTFDFRQIVAAVS
                     SGQFVLSPAVTNLLFGGFMFAFAVKAPLWPFHRWLPDAAVEATPASAVLMMAVMDKVG
                     TFGMLRYCLQLFPDASMYFRPLIITLAVIGIVYGAIVAIGQVDVMRLIAYTSISHFGF
                     IILGIFVMTSQGQSGSTLYMVNHGISTAALFLIAGFLVSRRGTRLINAYGGVQKVAPI
                     LAGTFLVSGLATLSLPGLAPFISEFLVLIGTFTRYPAFAVFASTALVLSAVYILWMYQ
                     RMMTGPVKDGNDRLHDLLPREIVVVAPLIALLLVLGVYPKPALDVINPAVNHTLTTID
                     QPDPAPKLAEGPSR"
     gene            complement(2819..4729)
                     /gene="nuoL"
                     /locus_tag="VFC01_11130"
     CDS             complement(2819..4729)
                     /gene="nuoL"
                     /locus_tag="VFC01_11130"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZN80224.1"
                     /translation="MTTLVWLVIALPVAGAAILLLSGRRTNSWGHLLGCLAALASFAA
                     GVVLFVDMLGRHAEDRIVHEILFSWVPVGGLHVDFGLQLDALSMCFVLLITGVGSLIH
                     IYSIGYMAEDPERRRFFAYLNLFLAAMLLLVLADNYLGLYVGWEGVGLASYLLIGFWA
                     HKPSAATAAKKAFVVNRVGDMGLAIALMVMFAYIGSISFAGVFSAAPRLGEGTLTAIG
                     LLLLLAACGKSAQVPLQSWLGDAMEGPTPVSALIHAATMVTAGVYLIVRSGPVFDLAP
                     NAQLGVVIVGAVTLLFGAVVGCAKDDIKKALAASTMSQIGYMVLAAGLGPAGYAFAIM
                     HLLTHGFFKAGLFLGAGSVMHAMNDEVDMRRYGGLRKALPITFATFGLGYLAIIGVPP
                     LAGFFSKDGIIEAALGAGGIKGPILGAAAILGAGITAFYMTRVMLMTFFGEKRWTSEA
                     SEATADKLVVHPHESPAVMTWPMILLAIGSVFSGGALAIGGTLQHWLEPVVGAHEEAH
                     ATPVWVATTVILVVVAAGIAIAYRMYATRAVPEEVPAGSALTVAARQDLYGDAFNERV
                     FMRPGQLLTEGLVEIDDDAVDGAANGLAAVVGRISNGFRQLQTGFARSYALSMLAGAA
                     LVVAAILAVQLW"
     gene            complement(4737..>4959)
                     /gene="nuoK"
                     /locus_tag="VFC01_11135"
     CDS             complement(4737..>4959)
                     /gene="nuoK"
                     /locus_tag="VFC01_11135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZN80225.1"
                     /translation="AIVMFMCVELMLNAANLAFVAFSRMHGHLDGQVVAFFTMVVAAC
                     EVVVGLAIIMTVFRTRRSASVDEASLLKH"
BASE COUNT         1037 a         1627 c         1608 g          687 t
ORIGIN      
        1 caggaacaac gaaaacacaa tgccgacaac gggataccgt cgacccagac cggcccaacg
       61 ttctaacgcc gtgtcctcct cgccgtcggg attgcgcacc agacccacca ccgcgaacgc
      121 acccacagtg ctgaacccat aggcgaacag atagaacagc gtcgacgata cgcccgcctc
      181 gttctgggcg atgacgccgg tgaggatgaa cccggtgtgc gccacggctg aatacgccag
      241 catccgcttg acatcgttct gagtcacggc agtgacggtg ccgaccacca tcgtcaggat
      301 cgcgatcgcc cacatcaccg ggcgccattc ggtatgcaga ccgggcaccg cgacgtagaa
      361 gatgcgcagc atcgcgccga atgcggcgat cttggtcgct gccgccatga acgcggtgat
      421 cggcgtcggc gcaccctggt acacgtcggg aatccacgag tggaacggca ccgccccgac
      481 cttgaaaagc accccgacga gcaccagccc ggtgccgatc agcgccagcg acgtcttgcc
      541 cgacccggac gacaccgcgt ccgcgatacc gccgagatcc agcgtgccgg cgtacccgta
      601 cagcatggca gcgccgtaca ggaagaaggc cgacgaaaac gcgccaagca gaaagtattt
      661 caccgacgct tcctgcgaga ggagccgccg ccgacgagcc aatccgcaca gcagatacag
      721 cggcagggac aacacctcga gcgcgatgaa catcgtcagc agatcatcgg cggcggggaa
      781 aatcagcatg ccgccgatgg cgaacatcgt cagcgggaag acctcggtct gcatgacacc
      841 ggccttggtg gccagctttt cggtgacact gcctacgatc gccgacgcct gcggcgtgaa
      901 cccgtccaga ccggggccgg tttccgcatc agccggcgcg gaaatgcgcc gttcggcgat
      961 gagcaggatg ccgagcacac cgaccagcag gatggtggcc tgaaggaata gtgccggggc
     1021 gtcgacagcg atggcgccca gcacggcggt gtgtccggtg cctccgtgca gatcccgagt
     1081 gagcccgacg accgcgacga acgcggcggc cagcccgccg aggctgagta ccaattgggc
     1141 ggcgtagcgc tgctgacgtg gcaggaacgc ctcgacgagg acaccgacga cggccacccc
     1201 gagcacgatc agcatggggg agagcaggcc gtattcgacg ctgggggtgg gcaggttcat
     1261 cgcgacggcc cttccgcgag cttcggtgcc gggtcaggct ggtcgatggt ggtcagcgtg
     1321 tggttgaccg ccgggttgat cacgtcgaga gcgggtttgg gatacacccc gaggaccagc
     1381 agcagcgcga taagaggtgc gacgacgacg atttcgcggg gtagtaggtc atgcaggcga
     1441 tcgttgccat ccttgaccgg gccggtcatc atccgctggt acatccacag gatgtagacc
     1501 gcggagagca cgagcgccgt cgacgcgaac accgcgaacg cggggtaacg cgtgaatgtt
     1561 ccgatcagga ccaggaattc gctgatgaac ggcgcgaggc cgggtagcga caatgtcgca
     1621 agaccggaaa ccaggaaggt gccggccaga atgggcgcga ccttctgcac gccgccgtag
     1681 gcgtttatca ggcgagtgcc cctgcgcgac accaggaatc cggcgatcag gaacagcgcc
     1741 gccgtcgaga tgccgtggtt gaccatgtag agcgtcgagc cggactgacc ttggctggtc
     1801 atcacgaaga tgccaaggat gatgaacccg aagtgggata ttgacgtata ggcgatcagt
     1861 cgcatcacat cgacctggcc gatcgcgacg atcgcgccgt agacgatgcc gatcactgcc
     1921 agcgtgatga tcaacggccg gaagtacatt gaggcgtcgg ggaacagctg caagcagtag
     1981 cgcagcatgc cgaaggtgcc gaccttatcc atcacggcca tcatcagcac cgcactggcc
     2041 ggggtggcct cgacggcggc gtcgggcagc caccggtgga acggccacag cggagccttg
     2101 accgcgaacg cgaacatgaa cccgccgaac aacaggttcg tcaccgccgg actcaggacg
     2161 aactgaccgc tggacaccgc agcgacgatc tgccggaagt cgaacgtgcc cgccgcgaac
     2221 acgtcgctct gcgctgtcac cacatacagg ccgataaccg cggcgagcat gatcaggccg
     2281 ccaaacaggt tgtacagcag gaacttcact gctgccttcg agcgattctc gccgccgaac
     2341 ccgccgatca ggaagtacat cgggatgagc atggcctcga agaacacgta gaacagcagc
     2401 acatccagcg agacgagcga catcagcacc atgccctcga ccgccagcgt cagcgcgaag
     2461 taggtgtgca ccgaccgtcc cgcccagccg atttggtcgc tggcgtcgtt ccagccggcg
     2521 atgatcagca gcggcacgag cacggtcgtc agcaccacga tggcgagcgc gatgccgtcg
     2581 acaccgagga tgtagccggt tccgaacgac ggtatccacc ggtggttttc gacgaactgg
     2641 tactggtcgc cccctggttt gaacccgacg gcgaccacca gggacaccgc gagcacagcg
     2701 agcgaggtca acagcgccag ccacttggcc accatgcgtt gcgaggcggg cagcaccatg
     2761 acgagtgccg cgccggccat cgggatcgcc cacagaatcg tcagccacgg gatggaggtc
     2821 accacagttg caccgccagg atcgcggcga ccacgagggc cgcgccggcc aacatcgata
     2881 gggcatagga gcgggcgaaa ccggtttgca gctgccggaa tccgttggag atgcgcccga
     2941 cgactgcggc cagcccgttc gctgcgccgt ccacggcatc gtcgtcgatc tcgacgaggc
     3001 cctcagtgag gagctggccg gggcgcatga acacccgctc gttgaacgcg tcgccgtaga
     3061 gatcctgccg tgccgccacc gtcagcgccg accccgccgg tacttcctcc gggaccgcgc
     3121 gggtggcgta catgcggtag gcgatcgcga ttcccgcggc aacgacgacg aggatcacgg
     3181 tggtggccac ccacaccggc gtcgcgtgtg cctcttcgtg cgcgccgaca accggttcga
     3241 gccagtgctg caacgtgccg ccgatcgcga gcgcgccgcc ggagaagacc gagccgatgg
     3301 ccaacaggat catcggccag gtcatgacgg caggtgactc gtggggatga acgaccagtt
     3361 tgtccgccgt cgcttcgctc gcctcagaag tccaacgctt ttcgccgaag aacgtcatca
     3421 gcatcacccg ggtcatgtag aacgcggtga tgcccgcgcc gaggatcgcc gcggcgccga
     3481 ggatcggacc cttgattccg ccggcgccga gcgcggcctc gatgatgccg tccttggaga
     3541 agaagccggc cagcggcggc acgccgatga tcgcgaggta gccgaggccg aacgtcgcga
     3601 acgtgatcgg cagtgccttg cgcaaaccgc cgtatcggcg catgtccacc tcgtcgttca
     3661 tggcgtgcat gaccgacccg gcaccgagga acaggccggc cttgaagaag ccgtgggtaa
     3721 gcaggtgcat gatcgcgaac gcgtatccgg ctgggccgag cccggcggcc agcaccatgt
     3781 agccgatctg cgacatggtc gacgccgcaa gcgctttctt gatgtcgtcc ttggcgcagc
     3841 cgacaaccgc cccgaacagc agtgtcaccg cgcctacgat gaccacgccc agctgtgcgt
     3901 tcggtgcgag gtcgaacacc ggaccggacc gcacgatcag gtacacaccc gcggtgacca
     3961 tggtggcggc gtggatgagc gcggacaccg gggtggggcc ctccatcgcg tccccaagcc
     4021 aggactgcag cggcacctgc gccgacttgc cgcacgcggc gagcaacagc agcagcccga
     4081 tggcggtgag ggttccttca ccgagccggg gagcggccga aaagacaccc gcgaacgaga
     4141 tcgagccgat gtaagcgaac atcaccatca gcgcgatcgc caggcccatg tcgccgacgc
     4201 ggttcaccac gaacgccttc ttcgccgctg tggccgccga cggtttgtgc gcccagaacc
     4261 cgatcagcag gtacgacgcc agacccacgc cttcccaccc gacgtagagg ccgaggtagt
     4321 tgtcggccag caccaacaac agcatcgccg cgagaaacag gttcaggtag gcgaaaaacc
     4381 gcctgcgctc gggatcttcg gccatgtagc cgatcgagta gatgtggatc agcgagccga
     4441 cgccggtgat cagtaggacg aagcacatcg acagtgcatc gagctgcagg ccgaagtcca
     4501 cgtgcaggcc accgaccggc acccacgaga agagaatctc gtggacgatc cggtcctctg
     4561 catggcggcc gagcatgtcg acgaacagca cgacgccggc ggcgaacgac gccagcgctg
     4621 ccaggcagcc gagcaggtga ccccagctgt tggttcgtct gcccgagagc aggaggatcg
     4681 cggcacccgc caccggcagg gcgatcacca gccacaccag agttgtcatg gcagccttag
     4741 tgtttcagca gactggcttc gtcgaccgaa gccgaacggc gggtgcggaa gacggtcatg
     4801 atgatcgcca ggccgaccac cacttcacac gcggcgacca ccatggtgaa gaacgccacc
     4861 acctggccgt ccagatggcc gtgcatgcgg gagaacgcga cgaacgccag gttcgccgcg
     4921 ttgagcatca actcgacgca catgaacatg acgatggcg
//
LOCUS       DASRJA010000243         5627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1187770,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000243 DASRJA010000000
VERSION     DASRJA010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187770"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..69)
                     /gene="rpmF"
                     /locus_tag="VFC01_11140"
     CDS             complement(<1..69)
                     /gene="rpmF"
                     /locus_tag="VFC01_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZN80226.1"
                     /translation="MAVPKRRMSRANTRSRRAQWKAK"
     gene            243..1949
                     /locus_tag="VFC01_11145"
     CDS             243..1949
                     /locus_tag="VFC01_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HZN80227.1"
                     /translation="MASVSTPVRIGNCSGFYGDRLAAMREMLTGGDLDYLTGDYLAEL
                     TMLILARDRAKSPERGYARTFLIQLEDCLGLAVDKGVRIVANAGGLNPAGLATAVRAL
                     ADRLGLTVNVAHVEGDDLVGRADELGFGTPLAANAYLGAWGIAECLRSGADVVVTGRV
                     TDASVIVGPAAAHFGWEPSNYDALAGAVAAGHVIECGAQATGGNYAFFTELADLTHPG
                     FPLAEIHADGSSVITKHPGTGGAVTVGTVTAQLLYEIGGARYANPDVTLRVDTIELSD
                     DGPDRVRISGVKGEPPPPTLKVSLNSIGGFRNEMALVLTGLDIEAKAALVRRQLETSL
                     TVKPAEMEWTLARTDHPDADTEEAASALLRCVVRDPDPANVGRQFSSAAVELALASYP
                     GFTSTSPPGDGQVYGVFTPGYVDATEVPHVAVHADGTRVDIAPAAQTLELAPVPPFAL
                     PEPLPFGETRRVPLGLIAGARSGDKGGSANVGVWVRTDDHWRWLVHTLTVEKLRELLP
                     ETADLPVTRHVLSHLRAVNFVVDGILGQGVAYQARFDPQAKGLGEWLRSRHVDIPEEF
                     LA"
     gene            1946..3097
                     /locus_tag="VFC01_11150"
     CDS             1946..3097
                     /locus_tag="VFC01_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80228.1"
                     /translation="MSIWHTPEREQLRKTVRAFAEREVLPHVDEWERSGDLPRELHRK
                     AGDAGLLGAGLPEAVGGGGGDGADSVIICEEMHYAGSPGGVFASLFTCGIAVPHMIAS
                     GDQRLIDTYVRPTLRGELIGSLAITEPGGGSDVGHLTTRAVRDGDDYIINGAKTYITS
                     GVRADYVVTAARTGGPGAAGVSLLVVDKDTPGFEVSRKLDKMGWRSSDTAELSYTDVR
                     VPAANLIGGENTGFAQIAGAFVSERVGLAAQAYSSAQRCLDLTLEWCRNRETFGKPLI
                     SRQQVQNTLAEMARRIDVARVYTHHLVERQLAGDDSNLIPHVCFAKNTAVEAGEWVAN
                     QAVQLFGGMGYMTESEVERQYRDMRILGIGGGTTEILTALAAKTLGFQS"
     gene            3094..4689
                     /locus_tag="VFC01_11155"
     CDS             3094..4689
                     /locus_tag="VFC01_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017351375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZN80229.1"
                     /translation="MTALRSTLDAKSSAYTEAASAMTAKLAELDAEHAKALAGGGPKY
                     VERHHDRGKLTPRERIELLLDPDAPFLELSPLAAYGSDFQVGASTVVGIGAVSGVECM
                     LVANDPTVKGGTSNPWTLKKVLRANQIAFENRLPVISLVESGGADLPTQKEIFIPGGQ
                     MFRDLTRLSAAGIPTIALVFGNSTAGGAYIPGMSDHVVMIKERSKVFLAGPPLVKMAT
                     GEESDDESLGGAEMHARTSGLADYFAVDELDAIRIGRRIVARLNWRKQGPTPSPVAEP
                     LFDAEELLGIVPSDLRVPFDPREVIARIVDGSEFDEFKALYGSSLVTGWATLYGYPIG
                     ILANHRGVLFSEESQKATQFIQLANRADTPLLFLHNTTGYMVGKAYEEGGMIKHGSMM
                     INAVSNSGVPHISLLIGASYGAGHYGMCGRAYDPRFLFAWPSAKSAVMGGTQLAGVLS
                     IVSRAAAAARGQQFDEDADAALRAAVEAQIEAESLPMFLSGRLYDDGVIDPRDTRTVL
                     GMCLSAIANGPIKGTSNFGVFRM"
     gene            4696..>5627
                     /locus_tag="VFC01_11160"
     CDS             4696..>5627
                     /locus_tag="VFC01_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HZN80230.1"
                     /translation="MITKVLVANRGEIARRVFDTCRRLGIGTVAAYTDPDAAAPHVAE
                     ADARVRLEGRNGYLDAGQLIAAARAAGADAIHPGYGFLSENPEFAAAVLEAGLKWVGP
                     PVAAVAAMGSKIEAKKMMAAAGVPVLDELDPDTVTADQLPVLIKASAGGGGRGMRVVT
                     DLDALPAQVEAARREAQSAFGDPTVFCERYLATGHHVEVQVLADEHGTVWAVGERECS
                     IQRRHQKIIEEAPSPLVERTPGMRDKLFDAARLAATAIGYTGAGTVEFMASPDGPDAA
                     SFYFLEMNTRLQVEHPVTEETTGLDLVELQLLVA"
BASE COUNT          912 a         1870 c         1938 g          907 t
ORIGIN      
        1 cttggccttc cattgcgcgc gccggctacg ggtgttcgcg cgcgacattc tgcgcttggg
       61 cacagccatg atcgagctct cctcatatag ggggtatgcg cgccgcgcgt ggtcgggcgt
      121 cggcaattgc tcttcaggat aaccggtgac ctgcataggc tcaaaacagc gatgaccttg
      181 actcgatacc gccggtaacc gctaacctac cggttggttg gtacacctgg atggaggact
      241 gcgtggcttc agtttcgacg ccggtccgca tcggcaactg ctcgggcttc tacggcgacc
      301 ggctggccgc gatgcgggag atgctgaccg gcggcgacct ggactacttg accggggact
      361 acctggccga gctgaccatg ctgatcctgg cccgcgaccg agccaagtcg cccgagcgcg
      421 gctacgccag gacgttcttg atccagctcg aggactgcct gggcctggca gtggacaagg
      481 gtgtgcggat cgtcgccaat gcgggcggtc tgaaccccgc cgggctggcc accgcggtgc
      541 gggcgctggc cgaccggctc ggcctcaccg tgaacgtcgc gcacgtcgag ggcgacgacc
      601 tcgtggggcg tgccgatgaa ctgggttttg gcacgccgtt ggcggcgaac gcgtatctcg
      661 gtgcgtgggg catcgccgag tgcctgcgaa gcggcgccga cgtggtggtc acgggtcgcg
      721 tcaccgacgc gtcggtgatc gtcggcccgg ccgcggccca cttcggctgg gaaccctcga
      781 actacgacgc gctggcgggt gcggtggccg cgggccacgt catcgagtgc ggtgcgcagg
      841 cgactggcgg gaactacgcc ttcttcaccg aactcgccga cctgacgcat cccggcttcc
      901 cgctcgccga gattcacgcc gacggctcct cggtgatcac caagcatccg ggcacgggcg
      961 gcgcggtcac ggtcggcacc gtcaccgccc agctgctcta cgagatcggt ggtgcccggt
     1021 atgcgaatcc cgatgtgacg ctgcgggtcg acaccatcga gctgtccgac gacgggcccg
     1081 accgggtgcg gatcagtggc gtcaagggcg agccaccacc gccgacgctg aaggtgtcgc
     1141 tcaacagcat cggcggcttc cgcaatgaga tggcattggt gctgacgggt ctggacatcg
     1201 aggccaaggc cgcactggtg cgcaggcagt tggaaaccag cctgaccgtc aagcccgccg
     1261 agatggagtg gacgctggcc cgcaccgacc accccgacgc cgacaccgag gaagccgcca
     1321 gcgcgctgct ccggtgcgtc gtgcgggatc ccgaccccgc gaatgtcggg cgccaattct
     1381 cctcggcggc agtcgaactc gcgctcgcca gctatcccgg attcaccagc acctcaccgc
     1441 cgggtgacgg tcaggtctac ggcgtgttca caccgggcta tgtcgacgcc accgaggtgc
     1501 cacacgtcgc cgtgcacgcc gacggcacgc gggtggacat cgcgccggcg gcccagacgc
     1561 tggagttggc acccgtgcca ccattcgcgc tgcccgagcc gttgccgttc ggggagaccc
     1621 ggcgagtgcc gctcgggctg atcgccggtg cacgcagcgg tgacaagggc gggtccgcca
     1681 atgtcggggt ctgggtgcgc accgacgacc actggcgctg gcttgtgcac acgctgaccg
     1741 tcgagaagct gcgtgaatta ctgccggaga cagccgattt gcccgtcacc cggcacgtgc
     1801 tgtcacacct gcgtgcggtc aacttcgttg tcgacggcat cctcggacag ggcgtcgcgt
     1861 atcaggcgcg gttcgaccca caggccaagg ggctgggcga atggctgcgc agccgtcacg
     1921 ttgacatccc agaggagttt ctggcgtgag catttggcac actcccgaac gcgagcagct
     1981 gcgaaagacc gtgcgcgcct tcgccgaacg tgaggtcctg ccacacgtcg acgagtggga
     2041 acgcagcggc gacctaccgc gcgagcttca ccgcaaggcc ggcgacgccg gtttgctcgg
     2101 cgccggactg cccgaagcgg tcggcggtgg cggcggcgat ggcgccgact ccgtgatcat
     2161 ctgcgaggaa atgcactacg ccggttcccc gggcggggtg ttcgcgtcgc tgttcacctg
     2221 cggcatcgca gtgccacaca tgatcgcctc tggagatcag cggctgatcg acacgtatgt
     2281 gcgcccgacg ctgcgcggcg agctgatcgg cagcctggcc atcaccgaac cgggcggcgg
     2341 ctccgacgtc ggccacctca ccacgcgcgc ggttcgcgac ggcgacgact acatcatcaa
     2401 cggcgccaag acctacatca cctcgggtgt gcgcgccgac tacgtcgtca ccgcggcccg
     2461 caccggcggc ccaggcgctg cgggtgtttc gctgctcgtc gtcgacaagg acacaccggg
     2521 attcgaggtc agccgcaagc tcgacaagat gggctggcgg tcgtcggaca ccgccgaact
     2581 gtcctacacc gacgtgcggg tgcctgcggc caacctgatc ggcggcgaga acaccggctt
     2641 cgcgcagatc gcgggggcgt tcgtgtccga gcgcgtcggc cttgccgcgc aggcgtattc
     2701 gagtgcgcag cgctgtctgg acctgacgct ggagtggtgc cgcaaccgtg aaacattcgg
     2761 taagccgctg atctcccggc agcaagtgca gaacacgctg gccgagatgg ctcgacgcat
     2821 cgacgtggcc cgggtgtaca cgcaccacct cgtggagcgc caactggcgg gtgatgattc
     2881 gaacctgatc ccgcacgtgt gcttcgccaa gaacacggcg gtcgaggccg gcgagtgggt
     2941 ggccaaccag gcggtgcagc tgttcggcgg catgggttac atgaccgagt ccgaggtcga
     3001 acgtcagtac cgcgacatgc gcattctcgg catcggcggc ggaaccaccg agatcctgac
     3061 cgcgctcgcg gcaaagacgt tgggattcca gtcatgacgg ctctgcggtc caccctcgac
     3121 gccaagtcgt ccgcctacac cgaggcggcg tcggcgatga ccgccaagct ggccgagctc
     3181 gatgccgagc acgccaaggc actggccggc ggcggaccca agtacgtcga gcgtcaccac
     3241 gaccgcggca agctcacccc gcgggaacgc atcgagctgc tgctcgaccc cgacgcgccg
     3301 ttcctggagc tgagcccgct tgccgcctac ggcagcgact ttcaagtcgg cgccagcaca
     3361 gtcgtcggta tcggcgcggt cagcggcgtc gaatgcatgc tggtggccaa cgatccaacg
     3421 gtcaagggtg gcaccagcaa cccgtggacg ttgaaaaagg tgctgcgcgc caaccaaatc
     3481 gcgttcgaga atcggctgcc ggtgatctca ctggtcgagt ccgggggtgc cgatctgccg
     3541 acccagaagg agatcttcat cccgggcggg cagatgttcc gcgacctcac ccggctgtcg
     3601 gcggcgggca tcccgaccat cgcgctggtg ttcggcaact ccaccgcagg tggcgcctac
     3661 atccccggca tgtccgacca cgtggtgatg atcaaggagc gctccaaggt gttcctggcc
     3721 gggccgccgc tggtgaagat ggccaccggt gaggagtccg acgacgaatc gctgggtggc
     3781 gctgaaatgc acgcccgcac ttcgggtttg gcggactact tcgcggtcga cgagctcgac
     3841 gcgatccgca tcggccggcg catcgtcgcg cggctcaact ggcgcaagca ggggccgacg
     3901 cccagtcccg tggctgagcc gctcttcgac gcggaggaac tgctcggcat cgtgccctcc
     3961 gatctgcggg tgccgttcga tccgcgggag gtcatcgccc gcatcgtcga cggctcggag
     4021 ttcgacgaat tcaaggcgtt gtatggctcg tcgctggtga ccgggtgggc gacgctgtac
     4081 ggctatccga tcggcatcct cgccaaccac cgcggcgtgt tgttcagtga agagtcgcag
     4141 aaggccacgc agttcatcca gctcgccaac cgcgccgata cgccactgtt gttcctgcac
     4201 aacacgaccg gctacatggt cggcaaggcc tacgaggaag gcgggatgat caagcacggc
     4261 tcgatgatga tcaacgccgt gtcgaactca ggagtcccgc acatttcgct gctgatcggc
     4321 gcgtcctacg gcgcgggcca ctatggcatg tgcgggcggg cctacgaccc gcggttcctg
     4381 ttcgcctggc caagcgcgaa atcggcggtg atgggcggca cgcaattggc gggcgtgctg
     4441 tcgatcgtca gccgggcggc ggcggccgcg cgcggtcagc agttcgacga ggacgccgat
     4501 gctgcgctgc gggcggcggt ggaggcgcag atcgaggccg agtcgctgcc gatgtttctg
     4561 tcggggcggc tctatgacga cggcgtcatc gacccgcgcg acacccgcac cgtgctggga
     4621 atgtgcttgt ccgccatcgc caatggcccg atcaagggga cgtcgaactt cggcgtcttc
     4681 cggatgtgag cccagatgat cactaaggtt ctggtcgcca accgcggtga gatcgcacgc
     4741 cgcgtgttcg acacctgccg gcgcctcggc atcggcactg tcgccgccta caccgatcca
     4801 gacgccgccg ccccgcacgt ggcggaggct gatgcccggg tgcgtctgga gggccgcaac
     4861 gggtatctgg atgccgggca gttgatcgcc gcggcccgcg ccgcgggtgc cgacgccatc
     4921 cacccggggt acggatttct ttcggagaac ccggaattcg ctgccgcggt cctcgaggca
     4981 ggcctcaagt gggtcgggcc gccggtggcc gcggtcgccg cgatggggtc gaagatcgag
     5041 gcgaagaaga tgatggccgc ggcgggcgtg ccggttctcg acgagctcga tcccgacacc
     5101 gtcaccgcgg atcagttgcc ggtgctgatc aaggcgtcgg ccggcggcgg tggccgcggc
     5161 atgcgggtcg tcaccgatct ggatgcgttg ccggcgcaag tggaggctgc gcgccgcgaa
     5221 gcgcagtccg cattcgggga cccgaccgta ttctgcgagc gctatctggc gaccggtcac
     5281 cacgttgagg tgcaggtgct cgccgacgag cacggcaccg tgtgggccgt cggcgaacgc
     5341 gaatgctcga tccagcggcg ccatcagaag atcatcgaag aggcaccgtc gcctcttgtc
     5401 gaacgcactc cgggcatgcg ggacaagctg ttcgacgccg cgcgccttgc ggcgaccgcg
     5461 atcggctaca ccggcgcggg cacggtcgaa ttcatggcca gcccagatgg tccggacgcg
     5521 gcgtcctttt atttcctgga aatgaacacc cgactacagg tcgagcatcc cgtcaccgag
     5581 gagaccaccg gcctggatct cgtcgagctg caactgctgg tcgccga
//
LOCUS       DASRJA010000244         3537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3658590,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000244 DASRJA010000000
VERSION     DASRJA010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3658590"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2305
                     /locus_tag="VFC01_11165"
     CDS             <1..2305
                     /locus_tag="VFC01_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZN80231.1"
                     /translation="ESITTNFVDINSIPVAAIERIEVLKDGASAVYGSDAVAGVVNII
                     MRDDFEGFEVSAGGGWTTESGYDETTVSAIWGMSGEDSNVTIIADYFKNSTLSNSERE
                     NSKGVSLGTANHSAEGGFDLRSSRGYPGFFIISDGVLEPDDEGNLVDSKAVIDPSCPP
                     DRTAGAVCVFDYGPFNLITPEAERTGLMLMAHQGFGNGLEVFTEIGVQHNNSLAQGAP
                     TPLDEGAELTVPITHPGNPFPEAIDIDVGRYRTVDAGARKWHISTDNLRGVLGLRGEI
                     NEWNWEVSAQRARSETEQSGGRSDGWVRVDFLQQQIDLFNYNPFNTTANPQSVIENIT
                     TSLVRRGESDLTSYDASIDGSLFDMPAGTVQMAAGLEYRDESISDIPDDQFQRGLIFG
                     TEAVSASASRDLWSAYVEFLVPLLESLELSLAGRYDDYSDFGDTTNPKVALRWSPIDS
                     LAFRASWGTGFRAPSLAQIGLGPSEESQFFRDTFACIDQNIDPNSSACVPLDYQIVFS
                     GNPNLDAEDSETFNVGAAWQPSSMWRVSLDYWDIKQEDKIDEVPFGFIYDLECNNQNS
                     TVCIRGNPVGNNTLGPLLELNSGFINIGEQSVSGLDLGANFASELGAGTLTLGLEYSH
                     LLEFERVELNSDATGFVTRDLTGEYEYPEDRFVLTGDYALESWDFYAAVNYTGEFQDA
                     PDADLDGTLDFDTVDTRDVEAFVTLNLQASWTGFEGLTLSLGVENALDEEVPFAIGDG
                     DVDVYGYVQNVHSPRGRFAYGKATYRF"
     gene            complement(2352..3362)
                     /locus_tag="VFC01_11170"
     CDS             complement(2352..3362)
                     /locus_tag="VFC01_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HZN80232.1"
                     /translation="MTSAPPTLLLSRSVIAGLATTQDYLASMHDAFTGLANQSYQLPD
                     AAYVPAKAGGFHVKSASRPGAGAMAVIKVNGNFPGNGVAWGLPTIQGFIAVLDAERGC
                     VLALMDSAEITARRTAATSALAARHLARSGSRSLGMIGCGLQALYHLDALLDVATIGL
                     VRYCDPRDDAAEAFGRRVTALGLEGKRAASAGAAARGADIVVTVTTSIHPVLALADVD
                     QGTFVAGVGADSPTKHELAPGLLRESRVVVDVRGQAVAGGDLGQAIRLGVMTEADVYA
                     ELADIVTGRVAGRSRDDERWVFDSVGLAVQDHAAARMILERASARDDLTSIWLDDSIG
                     PA"
     gene            3366..3503
                     /locus_tag="VFC01_11175"
     CDS             3366..3503
                     /locus_tag="VFC01_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80233.1"
                     /translation="MAHRCWADAPYKYRTVRALFAGGNGGQDVIVTPEFDLVFAPFGG
                     N"
BASE COUNT          646 a         1154 c         1138 g          599 t
ORIGIN      
        1 cgagagcatc accactaact tcgtcgacat caactcgatt ccggtggcgg ccatcgagcg
       61 gatcgaggtg ctcaaggacg gtgcgtccgc ggtatacggc tcggatgcgg ttgcgggtgt
      121 cgtcaacatc atcatgcgcg acgacttcga aggtttcgaa gtgtcggccg gcggcggctg
      181 gacgaccgag tcgggatacg acgagacgac cgtatccgcg atctggggca tgagcggcga
      241 ggactcgaac gtcacaatca tcgccgacta cttcaagaac agcacgctga gcaactcgga
      301 gcgcgagaac tccaagggcg tctcgctggg taccgccaac cactcagccg aaggtggctt
      361 tgacctgcgg tcctcgcgcg gctacccggg gtttttcatc atcagcgatg gagtgctcga
      421 accagacgac gaaggaaacc tcgtagactc gaaagcggtc atcgatccgt cgtgcccacc
      481 ggatcgcacc gccggcgcgg tgtgcgtgtt tgactacggg ccgttcaacc tcatcacccc
      541 tgaggcggag cgcaccggat tgatgctgat ggcccaccag ggattcggca atggtctcga
      601 ggtcttcacg gagatcggag ttcagcacaa caactcgctg gcccagggcg cgcccacgcc
      661 gctcgacgaa ggggcggaac tcaccgtgcc gatcacccat ccgggtaatc cgttcccgga
      721 ggcaatcgac atcgacgtcg gccgttaccg gacggtggac gccggtgcgc gcaagtggca
      781 catctcgacg gacaacctgc gcggcgtact cgggctgcgt ggcgagatca acgagtggaa
      841 ctgggaggtg tctgcccagc gcgcacgcag tgagactgag cagagcggtg gccggtcgga
      901 cggctgggtg cgggtcgatt tcctgcagca acagatcgat ctcttcaact acaacccgtt
      961 taacacgacg gccaacccgc agtcggtgat cgagaacatc accaccagcc tcgttcgccg
     1021 cggcgaatcc gacctgacct cctacgacgc cagcatcgac ggctccctct tcgacatgcc
     1081 ggcgggcacc gtgcagatgg cggcgggtct cgagtaccgt gatgagtcga tcagtgacat
     1141 acctgacgac cagttccagc gcgggctgat cttcggcacc gaagcggtgt cggcgtcggc
     1201 gtcgcgcgac ctgtggtcgg cgtatgtcga gttcttggta ccgcttctcg agagcctgga
     1261 gctgtccctc gccgggcgct acgacgacta cagcgacttc ggcgacacga cgaacccgaa
     1321 ggtcgcgctg cgctggtcgc ccatcgactc gctggcgttc cgtgcttcgt ggggcaccgg
     1381 gttccgcgcg ccctcgctcg cgcagatcgg cctgggccca tcggaggaat ctcagttctt
     1441 cagggacacg ttcgcctgca tcgaccagaa tatcgacccg aactcgagcg cctgtgtgcc
     1501 actggactac cagatcgtgt tctctggtaa cccgaacctg gacgccgagg attcggagac
     1561 cttcaacgtc ggcgcggcct ggcagccgag cagcatgtgg cgcgtgtcac tcgactactg
     1621 ggacatcaag caggaggaca aaatcgacga agtgcccttc ggcttcatct atgacctcga
     1681 atgcaacaac cagaacagca ccgtttgcat ccgaggcaac ccggtcggga acaacacact
     1741 cgggccgctg ctggagctca actcagggtt cataaacatc ggcgagcagt ccgtgagtgg
     1801 cctcgatctg ggggcgaact ttgcatccga gctgggcgcg ggcacgctga ctctgggcct
     1861 cgagtactcg catctgctcg agttcgagcg cgtggagctc aactcggacg cgaccgggtt
     1921 cgtgacccgc gacctgaccg gcgaatacga gtaccccgag gacaggttcg tactcaccgg
     1981 cgactatgcg ctggagagct gggacttcta tgccgcagtg aactacacag gggaattcca
     2041 ggacgcgccg gatgcagacc tggacggcac gttggacttc gacaccgtgg acacgcgcga
     2101 cgtcgaggcc ttcgtgacgc tcaatctgca ggcgagttgg acgggcttcg aaggcctcac
     2161 cctctcgctc ggtgtcgaga acgcgctcga cgaggaggtg cctttcgcga tcggcgacgg
     2221 cgacgtggac gtctacggct acgttcagaa cgtgcacagc ccgcgcggcc gcttcgccta
     2281 cggcaaggcc acctaccggt tctgacaggg acgtcagggg caaaggggcg cctccgggcg
     2341 ccccttttct ttcatgccgg gccgatgctg tcgtcgagcc agatcgaggt aagatcgtca
     2401 cgcgcgctcg cgcgctccag gatcatccgg gcggccgcgt ggtcctgtac cgcaagtccg
     2461 accgagtcga atacccaccg ttcgtcgtcg cgcgatcggc cggcgacgcg gccggtgacg
     2521 atatctgcga gctccgcgta cacatccgcc tcggtcatca cgccgaggcg gatagcctga
     2581 ccgaggtcgc cgcccgcgac cgcctgaccg cgcacatcga cgaccacacg gctttcccgc
     2641 agcagaccgg gcgcgagctc gtgcttggtg gggctgtcgg caccgacgcc ggcaacgaac
     2701 gtcccttggt cgacatcggc aagtgcgagc accgggtgta tcgacgtggt cacggtaacg
     2761 acgatatccg cgccgcgcgc tgcggcaccg gcgcttgccg cccgtttacc ctcgagacca
     2821 agcgccgtca cccggcggcc gaatgcctcg gccgcgtcgt cacgcggatc gcagtatcgg
     2881 accaggccga tcgtcgccac gtcgagcagc gcgtccaggt gatacagggc ctgcaggccg
     2941 catccaatca tcccgagact gcgcgacccg cttcgtgcga ggtgtcgggc ggccagggcc
     3001 gatgtggccg ccgtccgtcg tgccgtgatt tcggcggaat ccatgagcgc cagaacgcac
     3061 ccccgctcgg catcgaggac ggcgatgaaa ccctggatcg tgggcaggcc ccaggcaacg
     3121 ccattgccgg gaaaattgcc gttgaccttg atcaccgcca tcgcgcccgc gcccggccgt
     3181 gacgcgctct tcacatgaaa ccctccggcc ttcgccggca cataagccgc gtcgggcaac
     3241 tggtaactct gattcgccag gccggtaaac gcgtcgtgca tcgacgccag gtagtcctgc
     3301 gtcgtcgcca agccggcgat aacgctgcgc gacagaagca gggtgggtgg ggcagatgtc
     3361 atcgcatggc tcaccggtgt tgggcggatg ctccgtacaa gtaccgcacg gtgcgcgcct
     3421 tgttcgccgg tggcaacggc ggccaggacg tcatcgtgac ccccgagttc gatctggtgt
     3481 tcgcaccttt cggcggcaat taagccgtac ggatgagggt cagtcgcgcc gcgcggc
//
LOCUS       DASRJA010000245         5559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_795319,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000245 DASRJA010000000
VERSION     DASRJA010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795319"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VFC01_11180"
     CDS             <1..538
                     /locus_tag="VFC01_11180"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZN80234.1"
                     /translation="ASVIKTSAVAAEHRVVRAPAAVFDSQEEFLAAFQSGSLERDFVA
                     VLRYQGPQALGMPELHKLTPALGVLQDRGYRVALITDGRMSGASGKVPAAIHLTPEAA
                     ADGPLARVADGDVITLDADAGALTIHVPDEEFRTRTSTGRAPSVDEWVGTGRELFATM
                     RAAVGPADEGASVFRGIS"
     gene            535..1146
                     /gene="eda"
                     /locus_tag="VFC01_11185"
     CDS             535..1146
                     /gene="eda"
                     /locus_tag="VFC01_11185"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HZN80235.1"
                     /translation="MTSLLDIAPVIPVVVVHDAGDAVPIAKALVDGGLPIIELTLRTP
                     AALAAIERIANEVPEIIVGAGTIVETGQPKHALAAGAQFLVSPGSTPTLRAAMRDTGL
                     PHLPGVATVSEVLALLEDGYTDMKFFPAEAAGGAAYLRAIHSPVPAARFCPTGGITPT
                     NAAEYLKLPNVGCVGGSWLTRADAVEHRNWAHISDLARKAASQ"
     gene            1143..2351
                     /locus_tag="VFC01_11190"
     CDS             1143..2351
                     /locus_tag="VFC01_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN80236.1"
                     /translation="MTISLDRPVSVVAPDPAVRWLAVLALALGGFGIGTTEFVAMGLL
                     PDIASSLGITEPTAGHVISAYALGVVIGAPLIAALAARVPRKTLLLGLMAVFTLGNLA
                     SVLAPSYETLMIARFVAGFPHGAYFGVAALAATHLMGRHNRAKAVAHVMCGLTVATVI
                     GVPLASWLGQALGWRSAFGLVVAVGAATLTALWFGLPTQLRTMHVTSPLTELAALKRV
                     QVWLALAVGMIGFGGMFAVYTYISTTMTDVAGLPRSLVPLALMVFGGGMVVGNLVGGR
                     LADQSVIRALYVSMGALGIILAVFVLASHNPWTALLVLFGIGAAGSAVAPALQTRLMD
                     VAHDAQTLAAALNHSALNIGNATGAWVGGLVIAAGFGYTAPAAAGALLAATGLVVLTI
                     SVALQRRSLV"
     gene            2356..3117
                     /locus_tag="VFC01_11195"
     CDS             2356..3117
                     /locus_tag="VFC01_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN80237.1"
                     /translation="MAVLDKFRLDDKVVIVTGASSGLGVSFAQACAEAGADVVLAARR
                     VDKLYATSRLVEEAGRHALTVATDVADPAQCQAMVDAAMAEFGHVDVLVNNAGVGTAV
                     PATRETPNEFRAVVDINLNGSYWAAQAAAKVMQPGSSIVNISSVLGITTAGLPQAAYS
                     ASKAGISGLTRDLAQQWGPRKGIRVNAIAPGFFESEMTDAYKPGYIESLKPRVLLGRM
                     GDPAELAATLVWLASDAGGFVTGQTIVVDGGVTIT"
     gene            3212..3511
                     /locus_tag="VFC01_11200"
     CDS             3212..3511
                     /locus_tag="VFC01_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80238.1"
                     /translation="MKSYVTIGFTATSLASAALAFAAVAGAAPTGPSQVDDTVRTLEA
                     SGYNVIVNRTGAAPLSQCSVSAVRPGQTHSTEDSRGGSSINTTVTSKTVYVDVTC"
     gene            complement(3490..5010)
                     /locus_tag="VFC01_11205"
     CDS             complement(3490..5010)
                     /locus_tag="VFC01_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN80239.1"
                     /translation="MKRLIGIWRSWTLLRQLVVGVSAVVMIVLVTVGVTSVLSLRGSV
                     LGIIDAQLSGSADGFSQAVTKYRTTPDASGKVPPPGAMKPLTHLIGQAPGNVIALIQD
                     GKVVDSAYFVDGEARPAPKDAIAKIAELSWTGTEPRTVALPELGYYRMVARPGDGNEI
                     LVTGASRKPAWQAMTRETGIVAAITALALLVTAACTVAIVRFALRPLDRVAATAAEVA
                     TLPLDRDHHTIAPRVPEGDTDSRTEVGLVGDTLNRLLDHVGRALADVAASDRRMRQFI
                     TDASHELRTPLAAIHGYAELTRQDSAVLPETTEYSLARIEAEARRMNSLVSDLLLLAR
                     LDEGQDLDTADVDLTDMVVDAVNDAEVSAPDHRWLTNIPDAALWVRGDRARLHQTLAN
                     VLSNARVHTPAGTTVTTSIVTGRADDGSEYVELTITDDGPGIEPELLPHLFERFVRAD
                     KSRSREAGSFGLGLSIAASIIEAHDGTIEAESANGKTTFRMRLPTVSVAAQQVTST"
     gene            complement(5007..>5559)
                     /locus_tag="VFC01_11210"
     CDS             complement(5007..>5559)
                     /locus_tag="VFC01_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN80240.1"
                     /translation="TPDIIVLDIMLPDMDGLGVLQQIRDSGTYTPTLFLTARDSVMDR
                     VTGLTAGGDDYMTKPFSLEELVARLRGLLRRSAYLTPAADESLTVGDLELDAASREVT
                     RAGVPISLTSTEFELLRFLMRNPRRALSRKEILHRVWDYDFGGRSSIVDLYVSYLRKK
                     IDAQREPMIHTVRGVGYMLRPAE"
BASE COUNT          904 a         1860 c         1814 g          981 t
ORIGIN      
        1 tgccagcgtg atcaagacct ccgccgtcgc cgccgaacac cgggtggtgc gcgcacccgc
       61 cgcggtgttc gacagccagg aggaattcct cgcggccttc caatccggtt cgctggagcg
      121 cgatttcgtc gcggtgctgc gctaccaggg accgcaggca ctgggcatgc cggagttgca
      181 caagctgacc ccggcgctgg gggtgctgca ggaccgcggc taccgggtgg cgctgatcac
      241 cgacggccgg atgtccggtg cgtccggaaa ggtgcccgcc gcaattcatc tcacccccga
      301 agcggccgca gacggcccgt tggcccgggt ggccgacggt gacgtgatca ccctcgacgc
      361 cgatgccggc gcactgacca tccatgtgcc cgacgaagag ttcaggaccc ggacctcgac
      421 gggccgggca ccgtcggtcg atgaatgggt gggcaccggc cgcgaactgt tcgccaccat
      481 gcgggcggcc gtcggtccgg ccgacgaagg agccagcgtc tttaggggta tctcatgact
      541 tcactgctcg acatcgcacc cgtcattccg gtggtcgtcg tccacgatgc cggcgacgcg
      601 gtgccgatcg ccaaggccct cgtcgacggc ggactgccga tcatcgagct gacattgcgc
      661 acgcccgctg cgctggcggc catcgaacgc atcgccaacg aggtgcccga aatcatcgtc
      721 ggcgcaggca cgatcgtcga aaccggccaa cccaaacacg cgcttgccgc tggtgcgcag
      781 tttctcgtct cccccggcag cacccccacc ctgcgggcgg ccatgcgcga cacgggatta
      841 ccccatctgc ccggtgtggc gacggtatcg gaggtgctgg ccctgctgga agacggctac
      901 accgacatga agttcttccc ggcggaagcc gcgggtggcg cagcatactt gcgggctatc
      961 cactctcctg tgcccgccgc acggttctgc ccgacaggcg gcatcacccc gacgaatgcg
     1021 gcggaatacc tcaagttgcc caacgttggg tgcgtggggg gcagctggct tacacgggct
     1081 gacgccgtcg aacaccgcaa ctgggcccac atatcagacc tcgcacgaaa ggctgcctct
     1141 cagtgacaat ttcactggac cgccctgtct ccgtcgtcgc acctgatccg gctgtccggt
     1201 ggctggcggt gctcgccctg gccttgggcg gattcggcat cggcaccacc gagttcgtcg
     1261 caatgggcct gctacccgac atcgcgtcca gcctcggaat caccgaaccc actgcgggcc
     1321 atgtcatttc ggcgtacgcg cttggcgtgg tgatcggtgc cccgctcatc gcggcgctcg
     1381 ccgcgcgtgt gccacgaaag accctgttgc tcggcctgat ggcggtattc acattgggca
     1441 acctggccag cgtgctggca ccgtcgtacg aaacgctgat gatcgcccgg ttcgtcgccg
     1501 ggttcccgca tggcgcgtac ttcggggtgg cggcgctggc ggccacgcac ttgatgggac
     1561 ggcacaaccg cgccaaggcg gtcgcccacg tgatgtgcgg gctgactgtg gccaccgtga
     1621 tcggcgtgcc gctggcatcg tggctgggcc aggcactggg ctggcgcagt gcattcggcc
     1681 ttgtcgtcgc ggtcggcgct gcaacgctga ccgcactgtg gttcgggctg cccacccagt
     1741 tgcgcaccat gcatgtcacc agcccgctca ccgaactcgc tgcgctcaag cgtgtacagg
     1801 tctggctggc gctggcggtc gggatgatcg gcttcggcgg catgttcgcc gtgtacacct
     1861 acatcagcac gacgatgacc gacgtcgccg ggctgccgag atcgctggtt ccgttggcgc
     1921 tcatggtctt tggcggtggc atggtggtcg gcaatctggt cggcggtcgc ctcgcagacc
     1981 agtcggtgat tcgcgcgctc tatgtgtcga tgggcgcgct cgggatcatc ctggcggtgt
     2041 tcgtgctggc gtcgcacaat ccgtggacgg cgctgctggt gttgttcggc atcggtgccg
     2101 cgggttccgc ggtcgccccc gccttgcaga cccggttgat ggacgtcgcc cacgatgcgc
     2161 agacgctggc ggccgcgctg aatcactccg ctctgaacat cggcaacgcg accggagcct
     2221 gggtcggcgg attggtgatc gcggcgggat tcggctacac cgcgcccgcc gcagctggtg
     2281 cgctgctggc tgcgaccggc ctcgtcgtgc tcaccatctc ggtggcactg caacgacggt
     2341 cgctagtctg accggatggc cgtactcgac aagttccgac tcgacgacaa ggtcgtcatc
     2401 gtcaccggtg cctcgtccgg tttaggggtg tcgtttgccc aggcgtgcgc ggaggccggc
     2461 gcggatgtgg tgctggccgc caggcgcgtc gacaagctct atgccacctc gcgtcttgtc
     2521 gaggaggcgg gacggcatgc gttgacggtc gccacggacg tcgccgatcc cgcgcagtgc
     2581 caagcgatgg tggacgcggc aatggcggag ttcggccacg tcgatgtgct ggtgaacaac
     2641 gccggtgtgg gcaccgcggt gccggccacc cgcgagacgc ccaacgagtt ccgcgccgtc
     2701 gtcgacatca acctcaacgg ctcgtactgg gccgcgcagg ccgccgcgaa ggtgatgcag
     2761 ccgggcagct cgatcgtgaa catctcaagc gtgctcggca ttacgacggc cgggctacca
     2821 caagccgcgt atagcgcgag taaggccggg atctcgggcc tgacccggga tctcgcccag
     2881 caatggggcc cgcgcaaggg catccgcgtc aacgcgatcg cgccgggatt cttcgagtcc
     2941 gagatgaccg acgcctacaa gcccggctat atcgagagcc tcaagccgcg agtgttgttg
     3001 ggccgcatgg gtgatccggc cgagctcgcc gcgacgctgg tgtggctggc gtccgacgcc
     3061 ggcggcttcg tcaccggaca gaccatcgtc gtcgacggcg gtgtgactat cacctgacgg
     3121 tcgctacagc cagcggctca taccgtttac atatgtttgc gttcgctatg aagatgtggc
     3181 gccaagacgt agcgctccta gattgggatg tatgaaaagc tatgtcacca ttggatttac
     3241 cgcaacctcc ttggcttcgg cagcgctcgc attcgccgct gtcgccggcg cggcacctac
     3301 gggtccgtcg caggtagacg acactgtccg aacgctggaa gccagcggct acaacgtcat
     3361 tgtgaacagg accggcgccg ctccgctgtc acaatgcagc gtcagtgcag tccgtcccgg
     3421 gcagacgcac tccaccgagg attcacgcgg tggcagctcg atcaacacga cggtcacttc
     3481 gaagaccgtc tacgtagacg tgacctgctg agcggccacc gacactgtcg gcagtcgcat
     3541 tctgaaagtt gttttcccat tggcagattc ggcctcgatg gtcccgtcgt gcgcctcgat
     3601 gatcgatgcg gcgatggaca gcccgaggcc gaagctgccc gcctcccgag accgtgactt
     3661 gtcggcgcgg acgaaccgct cgaaaagatg cggcagcagt tcaggttcga ttcccgggcc
     3721 gtcgtccgta atggtcaatt ccacatactc cgagccgtca tcggctctgc cggtgacgat
     3781 tgacgtcgtt accgtcgtgc cagccggcgt atgaacccgg gcgttcgaaa gcacattggc
     3841 gagtgtttgg tggagccgcg cacggtcgcc ccgcacccat agcgcagcgt cgggaatgtt
     3901 cgtcaaccac cggtggtctg gcgccgacac ctcggcgtca ttcaccgcgt cgacgaccat
     3961 gtcggtgaga tccacatcgg cggtgtcgag gtcctgcccc tcgtcgagcc gggccaacag
     4021 gagaaggtcc gaaaccaacg aattcatccg gcgcgcttcg gcttcgatgc gagccaggga
     4081 gtactcggtg gtctccggaa ggaccgcgct gtcctggcgc gtcagctcgg cgtacccgtg
     4141 aatggctgcc agcggagtgc gcagttcgtg gcttgcgtcg gtgatgaact gccgcatccg
     4201 ccgatccgac gcggccacat cggccagcgc gcgccccacg tggtcgagca ggcgattcag
     4261 cgtgtcgccg acgagcccca cttcggtgcg cgaatcagtg tcaccctcag gcactctcgg
     4321 cgcgatcgtg tggtggtcac gatcaagcgg tagcgtcgcc acttcagcag cggtcgcggc
     4381 gacgcgatca agcgggcgca gcgcgaagcg gacgatcgcg accgtgcatg cggccgtgac
     4441 caaaagcgca agcgcggtga tggctgccac gatcccggtt tcgcgcgtca tcgcttgcca
     4501 tgccggcttg cgggaggcgc ccgtcacgag tatctcgttg ccgtcgccgg gcctggccac
     4561 catccggtag taacccaatt caggcagcgc cacggtgcgt ggttcggttc ccgtccaaga
     4621 caattcggcg atcttcgcga tcgcatcctt gggcgcgggc ctcgcctccc cgtcgacgaa
     4681 gtaggcggaa tcaaccacct ttccgtcctg gatcaacgcg atcacgtttc ccggcgcctg
     4741 cccgatgaga tgcgtgagcg gtttcatcgc gccgggtggc ggcaccttgc ccgacgcgtc
     4801 gggcgtggtg cggtacttgg tcaccgcctg gctgaaaccg tcggccgatc ccgagagctg
     4861 tgcgtcgatg atgcctagga ccgagccccg caagctcagc accgacgtca ccccgacggt
     4921 caccaggacg atcatcacca ccgccgagac gccgaccacc aactgccgca acaaagtcca
     4981 gctgcgccag atgccgatca agcgcttcat tctgctggcc gcagcatata gcccacgccg
     5041 cgcacagtgt gaatcatcgg ctcccgctgc gcgtcgatct tcttgcgaag atacgagacg
     5101 tacagatcta cgatgctcga ccgtccacca aagtcgtagt cccacacgcg atgcagaatc
     5161 tctttgcggc tcaacgctct tcgcggattg cgcatcagga accgcagcag ctcgaattcg
     5221 gtcgacgtca gcgagatcgg gacgcccgcg cgggtcacct cacggctcgc cgcgtccagt
     5281 tcgaggtcgc ccaccgtcaa gctctcgtcg gccgcaggcg tgaggtacgc cgaccggcga
     5341 agcagcccgc gcaacctggc caccaactcc tcgagggaga acggtttcgt catgtagtcg
     5401 tcgccgccag cggtcagacc ggtgacgcga tccatcaccg agtcgcgcgc ggtcaggaag
     5461 agcgtcggcg tgtacgtgcc ggagtcgcgg atctgctgca gcacgccgag gccgtccatg
     5521 tccggcagca tgatgtcgag aacgatgatg tccggcgtg
//
LOCUS       DASRJA010000246         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_60367,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000246 DASRJA010000000
VERSION     DASRJA010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60367"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFC01_11215"
     CDS             <1..406
                     /locus_tag="VFC01_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010841788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN80241.1"
                     /translation="TDSLNTLGPPLLFCVAFGLSMDYEVFMLSRIKEEYDRLGGDSAA
                     DNTAAVARGLESTGPIVTAAAVVMAIVFVSLATSGLTTVKSLGVGLAIAVLMDATIVR
                     ALLVPAFMRLAGRLNWWAPHRLQQREKVAVTA"
     gene            403..1347
                     /locus_tag="VFC01_11220"
     CDS             403..1347
                     /locus_tag="VFC01_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80242.1"
                     /translation="MTKHTITKTPPAHRLLAWGNERFGPAPILLYFLLYLAGILYARA
                     ITGDPNPSVSPLDVVAFSAVFGYFLLVRVLDEHKDYAKDSINFPDRILQRGLVTLDQL
                     KVIGILTLVPQIVVSVLVDGGIGPVTFAWLVLFGWTMLMLKEFFAGQWLEPRLVLYAL
                     THLVAGPLALIWIALIGADGKHLPASVGWLALMGFLGGATLEVGRKLHAPEDERDTVA
                     SYTKSFGGPAGAAAVLATLIVSTFANCAVLLAVARADTVVTTTVFVAVTIAAVAMTMQ
                     FASRPTARSTKLAELASALTVLVSLLVVITALLTGVRP"
     gene            1344..>2012
                     /locus_tag="VFC01_11225"
     CDS             1344..>2012
                     /locus_tag="VFC01_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HZN80243.1"
                     /translation="MTTLDMAEATDAGRIGGKAVGLTRLAAAGACVPWWIVVPTEAFE
                     RHITGIDPGRTAAHVRAQILSTPVDGELAATLTDRLTGHGPFAVRSSVVGEDGDTHSF
                     AGAFDTYLYVDADAVVDAVRRCWASAFNDRAVAYRRNTAAGTVPPAVAVIVQEMAEGD
                     VSGVLFTRNPLSGRDDEILVSAGWGLGEGIVSGQVDTDQYISSHAGLELSVTIADKAT
                     RVVRC"
BASE COUNT          314 a          687 c          645 g          366 t
ORIGIN      
        1 caccgactct ctgaacacgc tcgggccgcc gctactgttc tgtgttgcct tcggcctgtc
       61 gatggactat gaggtcttca tgctctcccg catcaaggag gagtacgacc ggctcggcgg
      121 cgacagcgca gccgacaaca ccgccgcagt cgcgcggggg ctcgagtcga ccggtccgat
      181 cgtcaccgca gcggccgtcg tcatggccat cgtgttcgtc tcgctcgcca cctccggact
      241 caccaccgtg aagtcgctcg gcgtcgggct cgcgatcgcg gtactcatgg acgcgacgat
      301 cgtgcgcgcc ctactggtac ctgcgttcat gcgtctggca ggcaggttga actggtgggc
      361 ccctcaccgg ctgcaacagc gagagaaagt cgcagtcacc gcatgaccaa acacacgatc
      421 acgaaaacac cgccggcaca ccggctgctg gcctggggaa acgaacggtt cggtcccgct
      481 cccatcctgc tgtacttcct gctttacctg gccgggatcc tgtacgcccg cgctatcacc
      541 ggtgacccga acccatcggt ctcgccgctc gatgttgtcg ccttcagcgc cgtcttcggc
      601 tacttcctgc tggttcgggt gctcgacgag cacaaggact acgccaagga cagcatcaac
      661 ttccccgacc gcatcctgca acgcggcctg gtcacgctcg accagctgaa agtgatcggc
      721 atcctcaccc tggtgccgca gatagtggtg tcggtgctgg tggacggcgg gatcggaccg
      781 gtcacgttcg cctggctcgt gctgttcggg tggacgatgt tgatgctgaa ggagttcttc
      841 gcgggtcagt ggctcgagcc caggctggtg ctgtatgcgc tcacccacct cgtcgccggt
      901 cccctggcgc tgatctggat cgcactcatc ggcgcggatg gcaagcacct gccggcgagc
      961 gtcgggtggc tggcgctgat gggcttcctc ggcggcgcga cactcgaggt cggccgcaag
     1021 ttgcacgctc ccgaagatga gcgggacacc gtcgcgtcct atacgaagtc gttcggcggc
     1081 ccggccgggg cggctgccgt gctggcgacc ctgatcgtca gcacattcgc gaactgcgcg
     1141 gtgctgctgg cggtggcgcg cgcggacacg gtcgtgacga cgacggtctt cgtcgccgtc
     1201 accatcgccg ccgtcgccat gacgatgcaa ttcgcgtcgc gaccaaccgc gagatccacc
     1261 aagctcgcgg aattggccag tgcgctcaca gttctcgtgt cactccttgt cgtgatcacc
     1321 gcactgttga ccggagtacg cccatgacca ctctcgacat ggccgaagca accgacgccg
     1381 gacgcatcgg cggtaaggcc gtcgggctga cccgactggc tgccgccggt gcgtgtgtac
     1441 cgtggtggat cgtcgtgccc accgaggcat tcgagcgcca catcaccggg atcgaccccg
     1501 gccgaacggc ggcacacgtt cgtgcgcaga tcctctccac accggtcgac ggcgaactcg
     1561 ctgcgacact caccgaccgg ctcaccggcc acggcccgtt cgccgtgcgc agttcggtcg
     1621 tcggcgagga tggcgacacc cattctttcg caggtgcttt cgacacatac ctctatgtcg
     1681 acgctgatgc cgtagtcgac gccgtgcgcc gatgctgggc gtcggcgttc aacgaccggg
     1741 ccgtggccta ccgccgcaac accgccgcgg gcaccgtgcc gccggcagtg gccgtgatcg
     1801 tccaggagat ggccgagggc gacgtctccg gcgtcctgtt cacacggaat ccattgtccg
     1861 gccgggatga cgagattctg gtcagtgccg gctggggtct cggcgaaggc attgtctccg
     1921 ggcaggtcga caccgaccag tacatctcgt cgcacgccgg cctcgagctg tcggtgacga
     1981 tcgccgacaa ggcgacgagg gtggtgcggt gt
//
LOCUS       DASRJA010000247         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3726587,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000247 DASRJA010000000
VERSION     DASRJA010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3726587"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1991)
                     /locus_tag="VFC01_11230"
     CDS             complement(<1..1991)
                     /locus_tag="VFC01_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZN80244.1"
                     /translation="MSNVIGIGAAPRRKEDFRFLTGRGNYVADIKRADMAFGVFIRSP
                     HGHAAIHGVDKAPALALPGVQAVLTGDDVAADGLGSLPCGWGIHGSDGLPMKEPAFPM
                     LAQGKVRFVGDMVAFVVADTLELARAAAEAVVIDYEVLPSVVGVLEAVRPGAPQLFDD
                     VPNNLCCDWELGDKAAVATAFKKAAHVARLSLVNNRLIGNPMEPRAAVAEYNGGTGHY
                     TLWTTSQFPHVVRFLMSALVLKIPEQKMRVVAPDVGGGFGVKQFHYGEEAVITWAAKL
                     VGRPVKWVADRTEGFISDRHGRDHVTEAELALDENGKFLALKVVTLANMGGYLSTFGP
                     NIPTNLYGPLLSGVYTTPAIYCNVKVVFTNTVPVDAYRGAGRPEATFVVERLVDVAAK
                     EMGIDRVELRRRNIIAKESYPYQTPVLVEYDSGDPMGCLEGALAEADVAGFAERKAAS
                     ERDGKFRGIGYSTYVEACGLAPSRFAGRLGARGGLYESATVRVHPTGQVTVLIGTHNH
                     GQGHETTFAQIVSEKLGVAFDNVDIVFGDTDRVQFGMGTYGSRSLVVGGAALSKASDK
                     VVLKGKKIAAHLLEASEHDIQFEAGVFSVAGTDRSKTFQEIAGAAYVPHDYPLEILEP
                     GLEEQAYYDPVNFTYPGGCHIAEVEIDPETGIVTLEKYTA"
     gene            complement(1994..2482)
                     /locus_tag="VFC01_11235"
     CDS             complement(1994..2482)
                     /locus_tag="VFC01_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN80245.1"
                     /translation="MIVSFTLNGRSRTVDVEPSALVADLLRENLNLTGTHVGCDTSQC
                     GACVVLFNGEAVKSCTLLAPMLDGGTLTTIEGLSGPGGSNVLHPMQQAFHQNHGLQCG
                     FCTPGMVMTAVALATKNPTPTEAEVRHGLEGNICRCTGYQNIVASVLAGAAAMHASGS
                     GE"
     gene            complement(2508..>2750)
                     /locus_tag="VFC01_11240"
     CDS             complement(2508..>2750)
                     /locus_tag="VFC01_11240"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HZN80246.1"
                     /translation="RDDIELVRADAGSPDDGATALPSRVTAIEYQGSFVKVMLDTVPD
                     EDFVAYVPERIFFHDPFNVGDVLLATWSAGRARVLA"
BASE COUNT          507 a          902 c          841 g          500 t
ORIGIN      
        1 gctgtgtatt tctccagcgt cacgatcccc gtctcgggat cgatctcgac ttcggcgatg
       61 tgacagccgc cgggataggt gaaattgacc ggatcgtaat aggcctgttc ctccagtccg
      121 ggctcgagaa tttcgagcgg atagtcgtgc ggtacataag ccgcgccggc gatctcctgg
      181 aatgtcttgc tgcggtcggt gcccgcaacc gaaaagaccc cggcctcgaa ctgtatatcg
      241 tgctcgcttg cctcgagcag gtgtgccgca atcttcttgc ctttcaggac gaccttgtcg
      301 ctcgccttcg agagcgcggc gccaccgacg accagcgatc gcgagccgta ggtgcccatg
      361 ccgaactgca cgcggtcggt atcgccgaat acgatgtcga cgttatcgaa ggcgacaccg
      421 agcttctccg acacgatctg cgcgaaggtg gtttcgtgcc cctgcccgtg attatgggtg
      481 ccgatcaaca ccgttacctg cccggtcgga tgcacccgga cagtggcgct ttcgtagagg
      541 ccgccgcggg cgccgaggcg tccggcaaag cgcgacggcg cgaggccgca ggcttcgaca
      601 taggtcgaat agccgatgcc gcggaacttg ccgtcacgtt ccgaagccgc cttgcgttcc
      661 gcgaagcctg cgacatccgc ttctgcaagc gcgccttcga ggcagcccat cggatcgccg
      721 gaatcatatt cgacgagcac cggcgtctga tacggatagg actccttggc aatgatattg
      781 cgacggcgca attcgacgcg atctatgccc atttccttgg ccgccacatc caccaggcgt
      841 tcgaccacga acgtcgcttc cggccgcccg gcgccgcgat aggcgtcgac cggcaccgtg
      901 ttggtgaaga ccaccttcac gttgcaatag atcgcgggcg ttgtgtaaac gccgctcagc
      961 aatggcccat agagattggt cgggatgtta gggccgaagg tggagagata accacccatg
     1021 ttggccagtg tgacgacctt caacgcgagg aattttccat tctcgtcgag cgccagttcg
     1081 gcctcggtga cgtgatcccg gccgtgccgg tccgagatga aaccctcggt gcggtccgcc
     1141 acccatttga cgggacggcc gaccagcttc gccgcccagg tgatcacagc ctcctcgccg
     1201 tagtggaatt gcttgacgcc gaagccccca cccacatcgg gcgccaccac gcgcattttc
     1261 tgctcgggaa tcttgagcac cagggcgctc atcaggaacc gcaccacatg gggaaactgg
     1321 ctcgtggtcc acagcgtata gtggccggtc ccgccgttgt actcagcgac cgccgcgcgc
     1381 ggttccatcg ggttgccgat cagtcggttg ttgacaaggc tgagacgcgc cacatgcgcg
     1441 gccttcttga acgccgtcgc aacggcggcc ttgtcgccga gctcccagtc gcagcagaga
     1501 ttgttgggaa cgtcgtcgaa cagttgcggt gcgcccggac gaacggcttc gagcacgccg
     1561 acgacggatg ggagcacctc ataatcaatg accaccgctt cggccgccgc gcgggcgagt
     1621 tccagcgtat cggcgaccac aaaggcgacc atgtcgccga cgaagcgcac cttgccctgc
     1681 gccagcattg gaaacgccgg ctccttcatc ggcaggccat ccgagccatg aatgccccag
     1741 ccgcaaggca gcgaaccgag gccgtccgcc gcgacatcgt caccggtcag cacggcctga
     1801 acgcccggca gcgccagtgc gggcgccttg tccacgccat ggatcgccgc atggccatgc
     1861 ggcgaacgga tgaacacacc gaacgccatg tcggcccgct tgatgtcggc gacgtaattg
     1921 ccacggccgg tcagaaagcg gaagtcttcc ttgcggcgcg gtgcagcgcc aattccgatc
     1981 acgttcgaca tggctattct ccgcttcccg acgcatgcat ggcggcagcc ccggccagca
     2041 ccgacgccac gatgttctga tacccggtgc agcggcagat attgccctcg aggccgtgac
     2101 gaacctcggc ttcggtgggt gttggatttt tcgtcgccag tgccaccgct gtcatcacca
     2161 tgcccggggt gcagaagccg cattgcaggc cgtgattttg atggaacgct tgctgcattg
     2221 gatgcagcac gttcgaaccg cccggcccgg agagaccttc gatcgtcgtc agcgtgccgc
     2281 cgtcgagcat cggcgcgagc aaggtgcagc tctttaccgc ctcgccgttg aacagtacga
     2341 cgcaggcgcc gcactggctg gtatcgcacc cgacatgggt gcctgtcagg ttcaggttct
     2401 cgcgcagcag atcggccacc agtgccgatg gctcgacgtc aaccgtccgc gaccggccgt
     2461 tgagcgtgaa actgactatc atgggtgacc tcccgtcatg agcgacatca ggcgagaaca
     2521 cgggcgcggc ccgccgacca agtggccagc agcacgtcac cgacattgaa ggggtcgtgg
     2581 aaaaaaattc gttccggaac gtaggcgaca aaatcctcgt caggcaccgt gtcgagcatc
     2641 accttgacga acgagccctg atattcgatc gcggttaccc ggctcggcag cgccgtcgcg
     2701 ccatcgtcgg ggctgccggc atcggcgcgc accagttcga tatcgtcgcg
//
LOCUS       DASRJA010000248         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1196856,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000248 DASRJA010000000
VERSION     DASRJA010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196856"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..642)
                     /locus_tag="VFC01_11245"
     CDS             complement(52..642)
                     /locus_tag="VFC01_11245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZN80247.1"
                     /translation="MTASTATGGAPLVVGLGGTLRANSSTERALRYCLESVERQGGRT
                     KFFSGPDLDLPMYTPHELKRTPKALELVNALRDADAVVVGSPGYHGAVSGLVKNALDY
                     IEDLREDPRVYLDNAPWGCISCAYGWQAAVGTLGQLRSIGHALRAWPTPLGVAINSAD
                     QIWDSAGQLVDTTVQGQLDMLATQLLTFARAAWETA"
     gene            complement(650..1642)
                     /locus_tag="VFC01_11250"
     CDS             complement(650..1642)
                     /locus_tag="VFC01_11250"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZN80248.1"
                     /translation="MTASMSDFRRVAGDVRNWGRWGDADELGTLNFITAEKVAEGASL
                     VKHGKVFPLGVDFGSSGPQGAFHFRQNPLHVMTIDGGDAATLLQYGPKWLQNPAAGQL
                     AGYFEGNPMRFNDDIIIMPLQAATQWDALSHVYYEDYLYNGFPADSVTSAGAFHCGID
                     KVDGKGITSRGVLLDIVRLRGVQTFCEIGDPITPDELDAAAKAQGVAITRGDIVLVRT
                     GWWARFLETGNGGEPGAGLDWRCASWLHDHEVAAVAADNLMVENPVSGVEGAFLPMHM
                     LCLRDMGLMLGEYWDLTALAADCAGDGVYEFQLIAPPLRVTGAVGSPINPIAIK"
     gene            complement(1639..>2098)
                     /locus_tag="VFC01_11255"
     CDS             complement(1639..>2098)
                     /locus_tag="VFC01_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN80249.1"
                     /translation="SGYRENAGVTAGLLDTVTMAEGIEWCQRNPEPVADGGGYRLSKE
                     AIILYGMANVATLGAKGIRINCTAPGVTDTPILDQLRSAYGQEFLDSFQTPLGRAADP
                     EEQASVLVFLNSHAARYITGQVIWVDGGSVAERVFGDVSDAREVERRRAQ"
BASE COUNT          359 a          701 c          660 g          378 t
ORIGIN      
        1 gatagctggt gaccagtccg ttgaccagaa tcgtcttgcg ttggatttcg gtcacgcggt
       61 ttcccaggcg gcacgggcga aggtgagcaa ctgggtcgcg agcatgtcga gctggccctg
      121 cacggtcgtg tctacgagtt ggcctgcgct atcccagatt tggtcggcag agttgatcgc
      181 gaccccgagc ggagtgggcc atgcccgcag cgcatgtccg atgctgcgca actgacccag
      241 cgtgccgaca gcggcctgcc acccgtatgc acagctgatg caaccccagg gagcgttgtc
      301 gagatagaca cgcggatcct cccggagatc ctcgatgtaa tccagcgcgt tcttcaccag
      361 tccggatacc gcgccgtggt atccgggcga gccgacgacg acggcgtcgg catcccgcag
      421 cgcattgacc agttcgaggg ccttgggtgt gcgtttcagt tcgtgcggcg tgtacatcgg
      481 cagatcgagg tccggtccgg agaagaactt cgtgcgtccg ccctgtcgtt cgacggattc
      541 caggcagtac cgaagcgcgc gctcggtgga cgagttggcc cgcagtgttc cgccgaggcc
      601 gacgacgagc ggcgcgccgc ctgtggctgt acttgcagtc atgtcctacc tacttgatgg
      661 caatcgggtt gatgggtgag ccgaccgcac cggtgacccg caacggcggc gcgatgagct
      721 ggaattcgta cacaccgtcg ccagcgcagt cggctgccaa cgccgtgagg tcccagtact
      781 cgccgagcat caggcccatg tcgcgcaggc acagcatgtg catcggcagg aatgcgccct
      841 cgacgccgga cacggggttc tcgaccatca aattgtctgc ggcgaccgcg gccacctcgt
      901 ggtcgtgaag ccacgaggcg cacctccaat caagaccggc gcccggttca ccgccgttgc
      961 cggtttccaa aaagcgtgcc caccaaccgg ttcggaccag cacgatgtcg cccctggtga
     1021 tggcgacgcc ctgggctttc gcggccgcgt caagctcatc cggggtgatc ggatcgccga
     1081 tttcacagaa ggtctgcacg ccgcgcagac ggacgatgtc gagcagcacg ccgcgcgacg
     1141 tgatgccctt gccatccacc ttgtcgattc cgcaatggaa ggcacccgcg ctggtcacgg
     1201 aatccgccgg gaatccgttg tagaggtagt cctcgtagta cacgtgcgac agcgcatccc
     1261 actgcgtggc ggcctgcagc ggcatgatga tgatgtcgtc gttgaaccgc atcgggttgc
     1321 cctcgaagta tccggccaat tgaccggcgg cggggttctg cagccacttc gggccgtact
     1381 ggagcaacgt ggcggcatca ccgccgtcga tcgtcatcac gtgcagcggg ttctgccgga
     1441 agtggaatgc gccctgcggc cccgacgaac cgaaatcgac gccgagcggg aagaccttgc
     1501 cgtgcttgac gaggctggcc ccctcggcga ccttctccgc ggtgatgaag ttcagggtgc
     1561 cgagttcgtc ggcgtcgccc cagcgtcccc aattgcggac gtcgccggcg actcgccgaa
     1621 agtcactcat gctggccgtc attgcgccct ccgcctctcg acttcccggg cgtccgacac
     1681 atcgccgaac acccgctccg ccactgaacc gccgtccacc cagatcacct gaccggtgat
     1741 gtatctcgcc gcgtgactat tgaggaacac cagcacgctc gcctgctcct ccgggtcggc
     1801 cgcgcggccc aaaggggtct ggaacgagtc caggaactcc tgcccgtatg cgctccgcag
     1861 ctgatcaagg atcggtgtat cagtgacgcc cggtgcggtg cagttgatcc ggattccctt
     1921 ggcgcccaac gtcgcgacat tggccatccc gtataggatg atcgcctcct tggacaaccg
     1981 gtagccacca ccgtcggcga ccggctcggg attgcgttga caccattcga tgccctcggc
     2041 catcgtcacg gtgtccagca gccccgcggt gacgccggca ttctcccgat aaccggac
//
LOCUS       DASRJA010000249         4039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3162343,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000249 DASRJA010000000
VERSION     DASRJA010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3162343"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..850
                     /locus_tag="VFC01_11260"
     CDS             275..850
                     /locus_tag="VFC01_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80250.1"
                     /translation="MTDPVDAATADPDEADAVEIIRVHRGQGDRRFSWRRVLAYGLLP
                     TVSVLLTTGAGYAKWMYSSAQASSAARIESVRAATDSAVAMLSYKPDTVEQDLTKARD
                     HLTGNMKDSYASLIHDVVIPGSRAKQISTAANVAAAASVSASENHAVVLVFVNQTITI
                     GNSAPTNTASSVRMTLDKRDGRWLVSQFDPV"
     gene            867..1238
                     /locus_tag="VFC01_11265"
     CDS             867..1238
                     /locus_tag="VFC01_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN80251.1"
                     /translation="MKRRTQLMALITAPMLLCLGMIAAGAPARADAVAYLINVTVRPG
                     YNFANADAALAYGLDVCGKVEQGRSYSQIVGDVWADFNTTDEFQASYLINQAVNELCP
                     ALIWQLRNSVSHYRPAGGANR"
     gene            1247..1717
                     /locus_tag="VFC01_11270"
                     /pseudo
     CDS             1247..1717
                     /locus_tag="VFC01_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003901302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
     gene            1766..2356
                     /locus_tag="VFC01_11275"
     CDS             1766..2356
                     /locus_tag="VFC01_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80252.1"
                     /translation="MASPRERMVKSAAVLIRERGGHPTAIADVLEHSRSPRGSAYHYF
                     PGGRTQLLCEAVDYAGRHLSSLIEGAASSVEAIDAFIGDYRQQLLSTGFRAGCPVAAV
                     VVEAGNPDDAINVTPTIEHAAAVFDQWTALIAQYLTADGVPSARSRDLALFVTASVEG
                     AIVLARAYRNIEPLDLVHARLSEMVGTVLCEATVKD"
     gene            complement(2837..3919)
                     /locus_tag="VFC01_11280"
     CDS             complement(2837..3919)
                     /locus_tag="VFC01_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZN80253.1"
                     /translation="MIVQGTSVGLDVHALSVVAHAVDEETGEIARARLCPDHGEILCW
                     LSQLRAPLRVVYEAGPTGFGLARALEDAGVECVVAAPSKLQRPSGDRVKTDARDAAHL
                     ARLLRLGEITAVRVPDREVEAVRDLVRAREDARADLMRVRHRLSKLLLRQGIVYFGGT
                     PWTCAHEEWLRRQRFDDIHLKAAFDHGFDAVLAATAARDRLDEQILEVAASPRFADPV
                     NRLGCLRGISALTGLALTVEIGDWSRFTGSSIGAYIGLVPSEYSSGNSRAQGSITKAG
                     NTHVRRLLIESAWHHRKSYSTSGPTLRARWAKVDPALRARGHAGNRRLNQRWRSFTER
                     GKNPLVANVAIARELAGWCWSLATMQ"
BASE COUNT          814 a         1258 c         1238 g          729 t
ORIGIN      
        1 tccaagcgtg cgcccgcgtt cattgatgtg gtcaacaggc ccactccaag acccaaggca
       61 ccgtcaccga ggcaggtctc gagcgggtcg aacgcgacca ggctcaggtg ttggtcgccg
      121 tcaccgtcaa cacatccaca gacagtgccc aagaacagca accgcgaccg tggcgcttgc
      181 gcattggtgt acagaaggtc ggcgacggcg ccaaagtttc caacgtggag tttgtgccgt
      241 gacgacgcag atgtccgcgg aaacagtgga cgcagtgacc gaccctgttg acgctgcgac
      301 tgccgatccg gatgaagccg acgccgtcga gatcattcgt gtccaccgcg ggcagggcga
      361 ccggcgcttc tcatggcgcc gagtactggc ctatggcctg ctgccgacag tgtcggtatt
      421 gctcacgacg ggcgccggct acgcgaagtg gatgtacagc tcggcacagg catcgagcgc
      481 ggcccggatc gagtctgtac gagcggccac agacagcgcc gttgcgatgc tgtcctacaa
      541 gccggacacg gtcgagcaag acctcacgaa ggcacgggac catctcaccg gcaacatgaa
      601 agactcatac gcgtcgctca ttcacgacgt ggtcatcccg ggatcacggg cgaagcagat
      661 ctcgacggcg gccaacgtgg cggccgcagc atcggtatct gccagcgaaa accacgcagt
      721 tgtattggtt ttcgtcaacc agaccatcac aatcggcaat tccgccccaa ccaacacggc
      781 atcgagcgtg cggatgacac tggacaaaag agacggcaga tggttggtct cgcagttcga
      841 cccggtctga ttgcgaaagg gtcagaatga aacgccgaac acaactgatg gcgctgataa
      901 ccgcaccgat gctgttgtgt cttggcatga tcgctgcggg cgcgcctgcg cgtgccgacg
      961 cggtcgccta cctgatcaac gtaaccgtcc gcccaggcta taacttcgcc aacgccgatg
     1021 ctgcgcttgc ctacgggctg gatgtctgcg gcaaggtcga gcagggccgc tcgtattccc
     1081 aaatcgtggg cgatgtttgg gccgacttca ataccaccga cgaatttcag gcgtcctatc
     1141 tcatcaacca ggcggtcaac gagttatgtc ccgcgctgat ctggcagcta cgcaactccg
     1201 tatcgcatta ccggccagca ggaggggcga accgatgatt ccaacagtgc gacgcgcggg
     1261 gataatcctc gtggttccaa cggttctcac tacgtgcctt gtcgtgcccg ccgcgaaagc
     1321 cgataacaag cggctcaacg agagcgtcgt gtcaaacgtg tacaccgttc agcggcaagc
     1381 tggatgcaca aacgacgtca aggtcagccc gcaactgcaa ctcgccgcgg aatggcacgc
     1441 aagggatgtg ctgaacaatc gtgaactcga tggcgatatc ggctccgacg gctcgacggc
     1501 ccaggatcgc gccaacgcca ccgggtttcg tggcaaggcc gccgaaacag tggcaatcaa
     1561 ccccgcgttg gccatcagcg gcatcgagtt gatcaaccag tggtactaca acccggccta
     1621 caaggcgatc atgtccgact gcgcgaacac agtcatgggg gtgtggtcgg agaacagcgt
     1681 ggaccgcacc gtggtcgttg cggtatacgg gcaaccgact agcactagct cgcccgctga
     1741 tcggtcgtaa aggagaattc agacattggc gagcccacgc gagcggatgg tgaaatcggc
     1801 cgcagtgctg atccgggagc gcggcggcca cccaacggcc attgccgatg tactggagca
     1861 cagtagatcg ccgcgaggtt cggcgtacca ctactttccc ggtgggcgta cccaattact
     1921 ctgtgaggcg gtcgattacg caggccgcca tctctcgtca ttgatcgagg gagcggcttc
     1981 gagcgtcgaa gccattgatg cgtttatcgg ggactaccgc cagcaactgt tgagcaccgg
     2041 atttcgggca gggtgcccgg tcgctgcggt ggtggtggaa gcggggaatc cggatgacgc
     2101 gataaatgta acgcccacca tcgaacatgc cgcggcggtt ttcgatcagt ggactgcctt
     2161 gatcgcgcag tacctgaccg cagacggcgt cccgtccgca cgctcccgag atctcgctct
     2221 gtttgtcacc gcatccgtcg aaggtgcgat cgtcctggcc cgtgcatatc ggaacatcga
     2281 gcccctcgac ctcgtgcacg cgcggttaag tgaaatggtc ggcacggtgt tgtgcgaagc
     2341 caccgtgaaa gactgagcag tcctcgcaca ctgagaaggt cgagccacgt acggcgagga
     2401 gaatcatgca caaccgtccg gttctaggat ccgccacgaa gctgagaaaa acgcatccgc
     2461 catttcgatg agaatcctgc aacgccgcag gtagagacgt cggtgtccgc cacgcagttt
     2521 gagaacctga tatgtgggcg aagttgtcaa gagctaggtg aaagccgccc cggtgcgagc
     2581 cggggcggct ttcgttttga ctgacctgat gtccgtgttg gcggtcgttg ctcacgacga
     2641 tggtgtcagg ctgatatgcg cgggttggtt accgcaggac gatgattcgg atgtgaacgg
     2701 tactgctgtc tacagtcgag cgtctctggc gatcgtcatt cgaatcgctt gtggctcata
     2761 ggttctttgc aggtttgttc acgcaccgcg ccaacgtcgg gctaccagga tcagtcaagt
     2821 attggatgct ggcgacctat tgcatggtgg ccagcgacca gcaccagccg gccagttcac
     2881 gcgcaatcgc gacgttggcc actagtggat tcttgccgcg ttcagtgaaa ctgcgccatc
     2941 gttggttgag gcgccgattg ccggcgtggc cgcgagcccg aagagcggga tcgaccttgg
     3001 cccagcgggc ccgcagggtg ggcccggagg tgctgtagga cttgcggtga tgccaggccg
     3061 attcgatcag tagccgtcgg acatgagtgt tgcccgcctt ggtgatcgac ccctgggcgc
     3121 gcgagttgcc cgaggagtac tccgagggca ccagccctat ataggcgccg atcgaagatc
     3181 cggtgaaccg cgaccagtca ccgatctcca ctgtcagggc cagaccggtg agggcggaga
     3241 tgccgcgtag acagcccagc cggttgaccg ggtcggcgaa ccgcggcgat gccgctactt
     3301 ccaggatctg ctcatcgagg cggtcccgtg cggccgtggc cgccagcacc gcgtcgaaac
     3361 catggtcgaa tgcggctttg agatggatgt cgtcgaagcg ctgccgccgc agccactctt
     3421 cgtgagcaca cgtccagggt gtgccgccga agtagacgat cccttgccgt agaagcagtt
     3481 tcgataaccg gtgccgcact cgcatcagat ccgcacgggc atcttcacgg gcccgcacca
     3541 gatcgcgcac cgcctcgacc tcccgatcgg gcaccctcac cgcggtgatc tcacctagac
     3601 gcagcaaccg agctaagtgt gcggcatctc gggcgtcggt cttgacccga tctcccgacg
     3661 ggcgctgcag cttcgacggc gcggccacca cacattcgac cccggcgtcc tcgagtgcgc
     3721 gagccagccc gaaccctgtc gggccggcct catagaccac tcgcagtggc gcgcgcaatt
     3781 gactcagcca acacaaaatc tcgccgtggt ccggacacag ccgtgcccgg gcgatctcgc
     3841 ctgtttcctc gtcaacggcg tgcgccacca ccgaaagtgc gtgcacatcc aacccgacac
     3901 tcgtaccctg aacaatcacc ggaggcctcc tgatctgcaa atgtggctct accagcgcga
     3961 gtgttcaccc acgccggcaa cccacgaatc gttttgcagc acgaggcctt cggcctcagc
     4021 tagcccctat accgtctac
//
LOCUS       DASRJA010000250         3954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2035791,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000250 DASRJA010000000
VERSION     DASRJA010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035791"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFC01_11285"
     CDS             complement(<1..742)
                     /locus_tag="VFC01_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN80254.1"
                     /translation="MWRTASCRKPHVRTSGGIVIVGGGLAAARTAEQLRRSEYTGPIT
                     IVSDEEHLPYDRPPLSKEVLRAETDDVTLKPAEFYDEHNITVLLGKAARSVDAAAQTV
                     TLADGSSIAYDELIIATGLVPKRIPSFPDLPGIHVLRSFDESLKLRGEAGSARNAVVV
                     GAGFIGCEVAASLRGLGVDVVLVEPQPAPLASVLGEQIGELVARLHRAEGVDVRCGVG
                     VAEVLGADKVEKVKLSDGAELDADLVVVG"
     gene            complement(767..1213)
                     /locus_tag="VFC01_11290"
     CDS             complement(767..1213)
                     /locus_tag="VFC01_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZN80255.1"
                     /translation="MERLDQTDERILAELAEDARATYAEIGQRVNLSAPAVKRRVDRM
                     LDNGVIRSFTTVVDRNALGWNTEAYVQVFRHGTIAPDELRKAWIDIPEVVSAATVTGT
                     ADAMLHVLARDMRHLEEALERIRSAADVERSESIVVLTNIIDRMRG"
     gene            1381..2190
                     /locus_tag="VFC01_11295"
     CDS             1381..2190
                     /locus_tag="VFC01_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HZN80256.1"
                     /translation="MKQRIPSVRHYAMTAPTFFAVEYAINPWMDTSTAVDTHVAMNQW
                     ETLRQTYKELGHTVELVEPVAGLPDMVYAANGGLLVNGRAVVARFAYPQRAGESAAYA
                     EWMARHGFEPAETRHVNEGQGDLLVVGSIVLAGYGFRTERAAHTEIAAHVRMPVVSLE
                     LVDPRFYHLDTALAVLDDTTIAYYPPAFSDDARTTLLELFPDAIEVAEADADVLGLNV
                     VSDGLSVVMPAAATGFAEQLRDAGFRPIGVDLSELLKGGGSVKCCTLEVHP"
     gene            2205..3437
                     /gene="rocD"
                     /locus_tag="VFC01_11300"
     CDS             2205..3437
                     /gene="rocD"
                     /locus_tag="VFC01_11300"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HZN80257.1"
                     /translation="MTGTDEAIALDGRYVAHNYSPLPVVAASAEGAWITDVEGRRYLD
                     CLAAYSAVNFGHRNPEILATAHAQLDTMTLVSRAFHSDRLGPFCEALAELCGKDMVLP
                     MNSGAEAVESGIKVARKWGHDVKGVPADKGTIVVAHNNFHGRTTTIISFSDDELARRG
                     FGPYTPGFLAVPFGDADAIANAIDDDTVAVLIEPIQGEAGIIVPPDDYLTRVRALCTE
                     RNVLMIADEIQSGLARTGRTFACEHWGVVPDIYLLGKALGGGVVPLSAVVADRDILGV
                     LHPGEHGSTFGGNPLAAAIGTTVVSMLQRGEFQARSTELGKRLHARLRALIGHGVLAV
                     RGMGLWAGVDIDPSIATGKQISMKLAERGVLVKDTQGSTLRFAPPLVITAEEIDWAID
                     QFADVLADTGSHLSGSSI"
     gene            3455..>3954
                     /locus_tag="VFC01_11305"
     CDS             3455..>3954
                     /locus_tag="VFC01_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN80258.1"
                     /translation="MTAPAQSLSEQLWRRRPVAGAPVAHGASDHLKRSIGTFQLTLFG
                     VGATVGTGIFFVLSAAVPEAGPAVLVSFVLAAIAAGLSAICYAEMASSVPVSGSTYSY
                     AYTTMGEFVAMGVAACLLLEYGVSISAVAVGWSGYLNKLLDNLFGWQLPQALTAAPWD
                     ETPGIV"
BASE COUNT          650 a         1353 c         1255 g          696 t
ORIGIN      
        1 tgccgacgac gacgagatcc gcgtcgagct cggcgccgtc gctcagcttg accttctcca
       61 ccttgtcggc tccgaggacc tcggcaactc cgacgccgca ccgtacgtcg acgccttcgg
      121 cgcggtgcag ccgcgccacc agctcgccga tctgttcacc gagcaccgac gcgagcggcg
      181 ccggctgcgg ctccaccaga accacatcga cgcccaggcc gcgcaggctg gccgcgacct
      241 cgcagccgat gaacccggcg ccgacgacga cggcgttgcg ggccgaaccc gcctcgccgc
      301 gcagcttgag gctttcgtcg aacgaccgca gcacgtggat gcccggcaag tccgggaacg
      361 acgggattcg cttgggcacc aagccggttg cgatgatcag ctcgtcgtag gcaatcgagc
      421 tgccgtcggc cagcgtcacc gtctgggcgg cggcatcgac agaacgcgcc gccttgccca
      481 acagcaccgt gatgttgtgc tcgtcgtaga actcggcggg cttgagggtg acgtcgtcgg
      541 tctcggcgcg cagcacttcc ttggacagcg gcggccggtc gtagggcaga tgctcctcgt
      601 cgctgacgat ggtgatcgga ccggtgtact cggatcgccg cagctgttca gcggtgcgcg
      661 cggccgccag cccgcccccg acgatgacga tgcccccaga tgttctcacg tggggttttc
      721 tacacgatgc cgtccgccat atggacgcca ccctgcggtt agccgctcag ccccgcatcc
      781 gatcgatgat gttcgtcaac accacgatgc tttcgctgcg ctcgacgtcc gcggcggagc
      841 ggatccgctc gagcgcctcc tccaggtggc gcatgtcgcg ggccagcacg tgcagcatcg
      901 cgtcggcggt gccggtgacg gtggcggcgc tgacgacctc cgggatgtcg atccacgcct
      961 ttcgcagctc gtcgggggcg atcgtgccgt ggcggaagac ctgtacgtag gcctcggtgt
     1021 tccagcccag tgcattgcgg tcgacgacgg tggtgaagct gcggatcacg ccgttgtcga
     1081 gcatgcggtc cacccgccgc ttaacggcag gtgcggatag gtttactcgc tgcccgatct
     1141 cggcgtatgt cgccctggca tcctccgcca gctcggccaa aatgcgttcg tcggtttggt
     1201 ccagacgctc catgatcacc tccacgcaac aaactgccgt atttttcagt gtgacacaat
     1261 agatcgctgg tcgacacgca acgaacggcg attgcttgca aattcttcag cttataccgt
     1321 ctaatcatga cgatttcaga tgtcgccgcc cttgcggccc cggtgtccga tgtcgccgca
     1381 atgaagcagc gaattccctc cgtccgtcat tacgcgatga cggccccgac cttcttcgcc
     1441 gtcgagtacg ccatcaaccc gtggatggac acctcgacgg ccgtcgacac ccacgtcgcg
     1501 atgaatcagt gggagaccct gcgccagaca tataaggaac tcgggcacac cgtggagttg
     1561 gtcgagcccg tcgccgggct gcccgacatg gtgtacgccg ccaacggcgg gctgctggtg
     1621 aacggcaggg ccgtggtcgc ccggttcgcc tatccgcagc gcgcaggcga atccgccgcg
     1681 tacgccgaat ggatggcgcg ccacggcttc gagcctgccg agacccgcca cgtcaacgag
     1741 ggccagggcg atctcttggt cgtcggctca atcgtgctgg ccggctatgg attccgcacc
     1801 gagcgtgcgg cccacaccga gatcgccgcg cacgtgcgga tgccggtggt cagcctcgag
     1861 ctggtcgatc cgcggttcta ccatctcgac accgcgctcg cggtgctcga cgacaccacc
     1921 atcgcgtact acccgccggc gttcagcgac gacgcacgca cgacgctgct ggagctgttt
     1981 cccgacgcga tcgaggtcgc cgaagcggac gcagatgtgc tcggcctcaa cgtggtttcc
     2041 gacggcctga gcgtggtgat gcccgcggcc gccaccggct ttgccgagca attgcgggac
     2101 gccggcttcc ggccgattgg tgtcgatctg tccgagctgc tcaagggtgg cggatccgtc
     2161 aaatgctgca cgctggaggt acatccgtga cgatccttga ctctatgacc ggtaccgacg
     2221 aagcgatcgc cctcgacggc cgctatgtcg cgcataatta ctcgccgctg ccggtggtgg
     2281 cggccagcgc cgaaggcgcg tggatcaccg acgtggaagg ccgccgctat ctggactgcc
     2341 tggccgccta ctcggcggtc aacttcggac accgcaaccc cgaaatcctc gcgacggcgc
     2401 acgcgcagct cgacaccatg acgttggtga gccgcgcctt ccactccgac cgtctcggac
     2461 cattctgcga ggctctcgcg gagctctgcg gcaaggacat ggtgctgccc atgaacagcg
     2521 gcgccgaggc cgtcgagagc ggtatcaagg tggcccgcaa atggggccac gacgtcaagg
     2581 gcgttcccgc cgacaagggc accatcgtgg tggcgcacaa caacttccac ggccgcacca
     2641 cgacgatcat cagcttttcc gacgacgagc tggcccgccg cggcttcggc ccctacacac
     2701 ccggcttcct ggcggtgccg ttcggtgacg ccgacgcgat cgccaatgcc atcgacgacg
     2761 acaccgtcgc ggtgctgatc gagcccatcc agggcgaagc aggcatcatc gtgccgcccg
     2821 acgactacct aacgcgggtg cgcgcgctgt gcaccgagcg caacgtgctg atgatcgccg
     2881 acgagatcca gtcgggcctg gcccggaccg gacgcacctt cgcctgtgaa cactggggcg
     2941 tcgtgcccga catctaccta ctcggcaaag cccttggcgg cggcgttgtt ccgctgtcgg
     3001 cggtggtggc cgaccgggac atactcggcg tgctgcaccc cggcgaacac ggctcgacct
     3061 tcggcgggaa cccgcttgcc gcagccatcg gcaccacggt ggtaagcatg ctgcagcgtg
     3121 gcgaattcca agcgcgctca accgaactgg gcaaacgcct gcacgcgcgg ttgcgcgcgc
     3181 tgatcgggca cggcgtgctc gctgtacgcg gcatggggct gtgggccggt gtcgacatcg
     3241 acccatccat cgccaccggc aaacagatca gtatgaagct ggccgaacgc ggcgtcctag
     3301 ttaaggacac gcaaggctcc acgctgcgct tcgctcctcc gctggtcatc accgccgagg
     3361 agatcgactg ggcgattgac caattcgccg acgttttggc cgatacgggc tcacacttat
     3421 ccggtagttc aatctaagcc ccgcaggagg caccatgacc gctccagcac aaagcctctc
     3481 cgagcaattg tggcgtcgcc ggcccgttgc gggtgcgccg gtcgcacacg gtgcttctga
     3541 tcacctcaag agaagtatcg gcacattcca gctgacactg ttcggtgtcg gcgcgaccgt
     3601 cggcaccggc atcttcttcg tgctctcggc ggcagtaccg gaggcgggcc cggcggtgct
     3661 tgtctcgttt gtcctcgccg ccatcgcggc cggcctctcc gcgatctgct acgcggagat
     3721 ggcatcttcg gtgccggtgt cgggctcgac atactcgtac gcctacacga cgatgggcga
     3781 attcgtcgcg atgggtgtgg cggcctgtct gcttctggag tacggcgtgt ccatctcagc
     3841 ggttgcggtg ggctggagcg gttacctgaa caagttgttg gacaacctgt ttggctggca
     3901 actcccacaa gcactgacgg ccgccccctg ggatgaaacg ccaggcatcg tcaa
//
LOCUS       DASRJA010000251         3483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_621931,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000251 DASRJA010000000
VERSION     DASRJA010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621931"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1284)
                     /locus_tag="VFC01_11310"
     CDS             complement(118..1284)
                     /locus_tag="VFC01_11310"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HZN80259.1"
                     /translation="MKTLSWLATGIAFSGFLIGVVHAAPPAPQARWTIMVYMSGDNNL
                     EDFIVKDIELELGLAGSNADIQIVALADRGPGYDTSRGNWQTTKLYHVTQGIVADAAS
                     AVADWGERNFGNPQTLIDFVTWTKTNYPADRYALYFWGHGWNWHPGWVMEDDTDVDTL
                     DYHEVKAAIPLLGFIDVVGYDGCNMATMEIYKLWQNKATAIAASEEYVGWNGIEYDSV
                     IAQLRANPFMSADQVAVASAQSAVVLGGERTFSAVAVDGRLANLLTATEQFAVALTNG
                     LAANRKKYDRAFGATRSMWQAPMDKDFYDLANEIDIAVSDANIKARAQGVMNAISSVR
                     LYEGHTNAYSDVHGMTIYHISKAAQKDANSTYYRSTIDLALTTSWDEFLNAYAQ"
     gene            complement(1423..2529)
                     /locus_tag="VFC01_11315"
     CDS             complement(1423..2529)
                     /locus_tag="VFC01_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80260.1"
                     /translation="MCLLAIPILTASTLGWNAVAQNTPGAIPDPGSYQGSVELQRREQ
                     ESAAQVQQQNEQMQQRIDQNYAAYAPQGGGGGGGGGVAARLKEKPLLPPAKNPLLGRW
                     RQMAGKPVDLGVLGAFPGGNEVVNGAFGGACKSVFGTGVVAFTPTALNWVAPDGHEEI
                     LNHVEYRSDGPNVIVIPSDPDPIPFIFGFPNRDHAVAAFFGCTMARVTDSAQPTAAKA
                     QPPSAAAPTASAILDLSIGEMVSGNFSPAPAGIQVYVTTQDPDANLVKAGFTSNPGVA
                     PIENLFAACKVDQGGSQERCNLGMAAMTAGAVGAASSDASGHAQTGALKPGRYYLIGF
                     TPYQGHALVWHMPVDLRAGSNSVTLSPQNGSMSH"
     gene            complement(2547..>3483)
                     /locus_tag="VFC01_11320"
     CDS             complement(2547..>3483)
                     /locus_tag="VFC01_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZN80261.1"
                     /translation="AIVPLFTLAPTELDFGDQAVGGTSAAQLVTLTNTGTVVLPISSI
                     GTTTSMFGVSHTCGTSVAVDASCAISVVFKPTSGGAKTATLRVTGGNGAGVETVALSG
                     TGIVPLYTLAPTALAFGDQAVGGTSAAQVVTLTNTGTVVLPISSIGTTTSIFGVSHTC
                     GSSVAVGASCTISVVFKPTSAGAKTGYVNVVGGNGAGTKTVALSGAGIVPLYTLAPTA
                     LAFGDQAVGGTSAAQVVTLTNAGTVMLPISSIGTTTSMFGVSHTCGTSVAVGASCTIS
                     VVFKPTSAGAKTGYLSVAGGNGAGTKTVALSGTGL"
BASE COUNT          586 a         1165 c         1073 g          659 t
ORIGIN      
        1 gactcggcct cgcccccgag cagctgccgg cgatcgagtt caagatcaag ttgcccaagt
       61 gacgcgagcg attgcgaacg ttctcctcgg tctcaacgac ggccgagccc gagcgcgtca
      121 ttgcgcgtag gcgttcagga actcgtccca gctcgtcgtg agcgcgaggt cgatggtcga
      181 acggtagtac gtggaattcg catccttctg ggcggccttg ctgatgtggt agatcgtcat
      241 gccgtgaacg tccgagtagg cgttcgtgtg cccctcgtag agcctgactg aagagatcgc
      301 attcatcacg ccctgcgccc tcgccttgat gttggcgtcg ctgaccgcga tatctatctc
      361 gttcgcgagg tcatagaaat ccttgtccat cggcgcctgc cacatcgagc gcgtggcacc
      421 gaacgcgcgg tcgtacttct tgcgattggc ggcgagtccg ttggtgagcg cgaccgcaaa
      481 ctgttcggtc gccgtcaaca ggttggcgag ccgtccgtcc acggccaccg ccgaaaaggt
      541 gcgctctcca cccaggacga ccgccgactg ggcgctcgcg accgcgacct gatcggcgct
      601 catgaatggg ttcgcacgca gctgcgcgat cacggagtcg tactcgatgc cgttccagcc
      661 cacgtactcc tctgaggccg cgatcgccgt ggctttgttt tgccacagct tgtagatctc
      721 catcgttgcc atgttgcagc cgtcgtaacc gacgacgtcg atgaacccga gcagcggaat
      781 cgcggccttc acctcgtggt agtcgagcgt gtcaacgtct gtgtcgtcct ccatgaccca
      841 gcccgggtgc cagttccagc catgccccca gaaatagagc gcgtagcggt cggccgggta
      901 gttggttttc gtccaggtta cgaaatcgat cagcgtctgg ggattgccga agttccgctc
      961 gccccagtcg gccacggcac tcgccgcatc ggcaacgatg ccctgcgtca cgtgatagag
     1021 cttggtcgtc tgccagttgc cgcgactcgt gtcgtaaccg ggtccacgat cggccagtgc
     1081 gacgatttgt atgtcggcgt tggacccggc aaggccgagt tccagctcga tgtccttgac
     1141 gatgaagtct tcgagattgt tgtcccccga catgtagacc atgatcgtcc agcgggcctg
     1201 cggggcgggc ggcgcggcat gcacgacgcc gatgaggaag cctgagaacg caatgccggt
     1261 cgccaaccac gataatgtct tcatggacgc tccctgggtt tcttgctcat tggatccggg
     1321 cagtgtcaaa actaccgcag tgctggctat tcgccaactc gttatacacc ccgcacgtat
     1381 ggcgtgaacg tgtcgttcca actgtgaagt tgcaacgtct ctttagtggc tcatgctgcc
     1441 gttctgagga ctcaaggtca ccgaattgga tccggcgcgc agatcgacgg gcatgtgcca
     1501 caccagtgcg tggccctgat agggagtgaa gccgatcaga tagtaccgcc ccggcttgag
     1561 ggcgcctgtc tgggcgtggc cagaggcatc ggagctcgct gcgccaactg cgccggcagt
     1621 catggcggcc attcccagat tgcagcgttc ctgactgccg ccttggtcga ccttgcaggc
     1681 ggcgaacaga ttctcgatgg gcgctacgcc ggggttcgaa gtgaaccccg ccttgaccag
     1741 gttggcgtcc ggatcctgag tcgtcacgta tacctggatg ccggcggggg ccggcgaaaa
     1801 attacccgac accatctcgc cgatactcaa gtcaaggatc gccgacgcgg tgggcgccgc
     1861 cgccgacggc ggctgagctt tcgccgcagt cggctgtgca ctgtcggtga cccgcgccat
     1921 ggtgcagccg aagaaggcgg cgaccgcgtg atcgcggttg ggaaatccga agatgaacgg
     1981 aattggatcg gggtcgctcg ggatgacgat tacattgggt ccgtcgctgc ggtattccac
     2041 gtggttgagg atttcctcgt gtccgtcggg cgccacccag ttgagcgccg tgggcgtaaa
     2101 tgcgactacg cctgtgccga acacagactt gcaggcgcca ccaaatgcgc cgttgacaac
     2161 ctcattgcca ccggggaacg cgcccaggac ccccaggtcg accggtttgc ccgccatctg
     2221 ccgccagcgt ccgagcaagg ggttcttcgc cggcggcagc aggggttttt ctttcaggcg
     2281 cgccgcgacg cctccgccac caccaccgcc cccgccctgg ggcgcatagg cggcgtagtt
     2341 ctggtcgatt cgctgctgca tctgctcgtt ctgctgctgg acctgcgccg cgctttcctg
     2401 ttcgcggcgc tgcaactcca cactgccctg gtacgagccg ggatcgggaa tggcccccgg
     2461 agtattttgt gccaccgcgt tccacccgag cgtcgatgca gtgaggatcg ggatggccag
     2521 cagacacaca cgcggcatgg tggctctcat aaccccgtgc ccgacagcgc gaccgtcttg
     2581 gtgcccgcgc cgttaccgcc cgccacgctc agataccctg tcttcgctcc cgcactggtc
     2641 ggcttgaaca ccacgctgat cgtgcaactc gcgccaaccg ccaccgacgt accacaggtg
     2701 tggctcacgc cgaacatact ggtggtcgtg ccaatcgagc tgatcggcag catcacggtc
     2761 ccggcgttcg tcagcgtcac cacctgcgcc gccgaggtcc cacccaccgc ctgatcgccg
     2821 aaggcgagtg cggtcggcgc cagcgtatag agcggcacga tgcccgcgcc cgacagcgcg
     2881 accgtcttgg tgcccgcgcc gttgccgccc acgacattca cataccctgt cttcgctccc
     2941 gcactggtcg gcttgaacac cacgctgatc gtgcaactcg cgccgaccgc caccgacgaa
     3001 ccacaggtgt ggctcacgcc gaatatgctg gtggtcgtgc caatcgagct gatcggcagc
     3061 accaccgtcc cggtgttcgt cagcgtcacc acctgcgccg ccgaggtgcc acccaccgcc
     3121 tgatcgccga aggcgagcgc ggtaggcgcc agcgtataga gcggcacgat gcccgtgccc
     3181 gacagcgcga ccgtctcgac gcccgcgccg ttgccgccgg tcaccctcag agtcgctgtc
     3241 tttgcgcccc cactagtcgg cttgaacacc acgctgatcg cgcaactcgc gtcgaccgcc
     3301 accgacgtac cacaggtgtg gctcacgccg aacatgctgg tggtggtgcc aatcgagctg
     3361 atcggcagca ccacggtccc ggtgttcgtc agcgtcacca gctgcgccgc tgaggtccca
     3421 ccgaccgcct gatcgccgaa gtcgagctcg gtcggcgcca gcgtgaagag cggcacgatg
     3481 gcc
//
LOCUS       DASRJA010000252         9823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3420711,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000252 DASRJA010000000
VERSION     DASRJA010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9823
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3420711"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..430
                     /locus_tag="VFC01_11325"
     CDS             227..430
                     /locus_tag="VFC01_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZN80262.1"
                     /translation="MAQGTVKWFNGDKGFGFITPDDGAKDVFVHFSEIQGSGFRSLEE
                     NQRVQFEVEQGAKGPQAVRVSAI"
     gene            complement(501..998)
                     /locus_tag="VFC01_11330"
     CDS             complement(501..998)
                     /locus_tag="VFC01_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5994 family protein"
                     /protein_id="HZN80263.1"
                     /translation="MAGPRRLARPVRLALAPQLGADIDGAWWPYTASLSDELPGLIEA
                     LHRPLGEIVDICVNWSATEGPRELYEMAASARLTSPGKRRHHRLMVIDGRLACAKLLV
                     VPHMTSLMLGLMMMRFAAAMPVSDSRQDSQMYDICDSVMQSARIESASWTRRMREMAA
                     TADPR"
     gene            complement(1252..1881)
                     /locus_tag="VFC01_11335"
     CDS             complement(1252..1881)
                     /locus_tag="VFC01_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid-like protein"
                     /protein_id="HZN80264.1"
                     /translation="MRAARLDGVLHSVASAPLTFLWLVVLLVTTRVQRSAGPGGSQQL
                     QHDHSTNLRWLRSQPLRVLATSLFWLDERKWWPNVPVFVAVVAPAERRLRWRRWLLVG
                     IAAHVIATFVGQGYLRLLITKKKAPRWLVNARDVGVSYFVFGVAGALSGYAPRPWRSR
                     CQVAVLVALAGNAAARPTFTQLGHLTAFAVGLAAIPLAPDREHMTYPRV"
     gene            complement(1990..3783)
                     /locus_tag="VFC01_11340"
     CDS             complement(1990..3783)
                     /locus_tag="VFC01_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZN80265.1"
                     /translation="MDATPFGHYQLQKLIGRGGMGEVYRAYDTKTDRIVALKVLPHHL
                     AEDVTFQQRFRRESQAAAGLNDPHVVPIHGYGEIDGRLYLDMRLIEGRNLGTMLEEAE
                     GKPLGAAFAVTVVTQVANALDAAHTAGLIHRDVKPSNILITDRDFVYLIDFGLARTAG
                     EKGLTTAGNTLGTLAYMAPERFEGADIDPRADIYALTCVLYECITGSRPYPAESYEQQ
                     IKGHMVSPPPRPSAIDPKLSAFDDVIAKGMAKKPAKRYQTAGELAAAARRAQETPVRT
                     TGRSGKHSRQRSQGVSKRAMAIAAAVVLVAAVGTFGVLQWRGSRGGDTRPASVAESSA
                     TPAPVTGAVPEIAATVPEDIRKSGRLVIGVNVPYAPNEFKNSSGEIVGFDVDLMNAVA
                     KTLGLTPDYRETAFESIMPSVSSGSFNAGMSSFTDTKEREADVDFVTYFQAGTLWAQR
                     AGSTVDPEAACGLKIGVTYASIQETEEVPAKSDQCVAAGVSPIDKVVYTRQDDLTAAL
                     IAGEVEAMAADSPVTGFAVKLSGGQLAPAGEIFDTAPYGWPVAKGSGLAEALQRALEH
                     LMQTGEYKTIATMWGVEKGMIDKPVINGAVR"
     gene            4013..4612
                     /locus_tag="VFC01_11345"
     CDS             4013..4612
                     /locus_tag="VFC01_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80266.1"
                     /translation="MQWLTVAWWRWRLAQVRGRNPLVRRSDRIDVAVTALAVVVSLAV
                     IPFALAIGTAVHDENARTYAAQQHDRHKVVATATADSTGGAGNPKGPVAVVSARWQVG
                     DVENAGAFTWDSPVKDGSDMDIWVDRNGRQVGPPAASWQAGVDAAVAAVGMWLAVTAA
                     AALLVAAVRLGLRRARYAGWNRDMVSLVHDNGGRTNRRR"
     gene            4654..5136
                     /locus_tag="VFC01_11350"
     CDS             4654..5136
                     /locus_tag="VFC01_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80267.1"
                     /translation="MAEQPAVFVSHPPERLLRAVNPVMRFLLGTPLAGPARKQMMVVS
                     FTGRKSGRQYSIPLSAHVIDNELYALTSAPWKNNFRDGADAEVLNDGKRTPMRGELVT
                     DPAIVADLSHRCAESYGVKRAQRMMGLGFRDQRIPTVDEFKEAAVRERIVAVKFTPTT
                     "
     gene            5136..5522
                     /locus_tag="VFC01_11355"
     CDS             5136..5522
                     /locus_tag="VFC01_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HZN80268.1"
                     /translation="MAERNVLGGPLEPCGTEPLTGFYRDGCCSTGQEDIGLHTICAVV
                     TAEFLEHQSSIGNDLSTPRPEFRFPGLTPGDRWCVTAANWFRAYQAGCAAPVVLASTH
                     ERTLDLVSLDALQKHAVDVPDDLEGL"
     gene            5599..6594
                     /locus_tag="VFC01_11360"
     CDS             5599..6594
                     /locus_tag="VFC01_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN80269.1"
                     /translation="MDINRRSFIGLTGAAGLAVLTAQGSQLASAAPASGPVTIDHAFG
                     QTTIPAPPKRVVSAGFTEQDDLLALGVVPIATTAWWGDEPFEVWPWARPKLGNAQPTV
                     LSIYSDAGFEFDRIAGLNPDLIVATNAGVDQDKYNRLSAIAPTIPQSAGQPPFFEPWQ
                     VQANTIGQAVFKAGEMASLVAAAERKLTDVARANPQFAGKTVQMMQGTFFDDGAWATL
                     PGWRTNFLTDMGLVVPDTLRAFTFDDHRAFIPRDQVAAALGGVDVLIWTTQSPEDQAG
                     LLAEPSVQQLNAASPNRSVYTGRNLAAAIAFSSPLSLPVVADQLPPLLKTAVIAA"
     gene            6674..7462
                     /locus_tag="VFC01_11365"
     CDS             6674..7462
                     /locus_tag="VFC01_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HZN80270.1"
                     /translation="MDNLATAAQSQLDQWQLRRDGDPIQGADSLVLPVRTSHGAHAIL
                     KMSQSEHEHLVLRRWGGSGAVRLLSADPPHRALLLERLRPESLDTLSDVDACEVVAGL
                     YRRLHVPALPQLHTLTSYLEQWTRDFEVLPRSAPIPHRLVEQAIALGRDLAAEPGASV
                     VHGNLHYENVLAAVREPWLAIAPRPVNGDPHYEIAPMLWTRWDELSGNIRDGVRRRFY
                     TLVDAAGFDEERARAWVVVRVVQQAIRDPMSLTKYVALAKAVQD"
     gene            7643..8563
                     /locus_tag="VFC01_11370"
     CDS             7643..8563
                     /locus_tag="VFC01_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HZN80271.1"
                     /translation="MAETHIKAAQSKWLAKSAAQTRGSDKKEVQFHYDISNEFFKLWQ
                     DPTQTYSCAYFERDDMTLEEAQMAKVDLSLGKLGLQPGMTLLDIGCGWGSTIMRAVDK
                     YDVNVIGLTLSENQKQHIEDYWFANSTSKRHMEVRLQPWEEFDGKVDRVVSIGAFEHF
                     GFNKYDDYFKKTYSWMPDDGVMLLHTIIIPSDDEIKAKKLPLTMSNVRFIKFIMDEIY
                     PGGRLPLAAQVEEHAVKAGYTVTRKQKLQPHYARTLDTWAANLESKKDEAIAITSEEV
                     YERFRKYLTGCADLFRNGYTDVCQFTCEKR"
     gene            8686..9540
                     /locus_tag="VFC01_11375"
     CDS             8686..9540
                     /locus_tag="VFC01_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HZN80272.1"
                     /translation="MRPHFEEIQAHYDLSDDFFGTFQDPSRTYSCAFYEREDMSLEEA
                     QLAKIDLALDKLELRPGMTLLDVGCGWGSVMKRAVEKYDVNVIGLTLSKNQAVYCEHL
                     LEEIESDRSRQVFLRGWEEFDQPVDRIVSIEAFEAFPKERYAAFFETCHRILPTDGRM
                     VLQTILGHPLKRWPDMGIPITMTDLKFMRFIAKEIFPGGAIPCDEDIANYSGAAGFSL
                     VQTQYLNEHYVRTLETWALNLEAAQDEAVVATSEEVYQRYMRYLTGCADFFQRGICEL
                     GQFTLTKG"
     gene            complement(9544..>9823)
                     /locus_tag="VFC01_11380"
     CDS             complement(9544..>9823)
                     /locus_tag="VFC01_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854602.1"
                     /note="FabF; beta-ketoacyl-ACP synthase II, KASII;
                     catalyzes a condensation reaction in fatty acid
                     biosynthesis: addition of an acyl acceptor of two carbons
                     from malonyl-ACP; required for the elongation of
                     short-chain unsaturated acyl-ACP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HZN80273.1"
                     /translation="IKYAFGDHTPAVYAPKAALGHPLGAAGAVESVLAVQALRDGVVP
                     PTLNLKDLDPEIDLDVVAGSPRRADFRYAVTNSFGLGGNNVAVVFGAA"
BASE COUNT         1855 a         3291 c         2973 g         1704 t
ORIGIN      
        1 tcgccagggt tgatgtcgtc atctcaggtc tcccttgacc aggcccctcg tttcgccatc
       61 gtaaggcccg ataacgccga cgcgatcgat attgcccacc gggctcccgc acctaagacc
      121 acagccgcgc cccaggaaca cagcacgcga gctgagtcgc gtaccagctg ctacggtaag
      181 cgcggatgtt ttaggtacat ccgcattgaa tgagagaagt aagtaaatgg cacagggaac
      241 tgtgaagtgg ttcaacggcg acaagggctt cggtttcatc acccccgatg acggcgcgaa
      301 ggatgtcttc gtgcactttt ccgagatcca gggaagcggc tttcgcagcc tcgaggagaa
      361 ccagcgcgtt caattcgagg ttgagcaggg agccaaggga ccccaggcgg taagagtcag
      421 cgccatataa gcgacccata catgacggtg ggctggtgcg gatagttccg tatcagccca
      481 ttggtcgttt ccatactccc ttagcgcggg tccgcggttg cggccatctc acgcatacgt
      541 cttgtccacg aagcactttc gatccgcgcg ctttgcatta cggaatcgca aatgtcgtac
      601 atctgactgt cttgtcgtga gtcggaaacc ggcattgccg ccgcaaagcg catcatcatg
      661 aggccaagca ttagcgacgt catgtgcggg acgacaagga gtttcgcgca cgccagccga
      721 ccgtcgatca ccatcagccg gtgatgccga cgcttcccgg gggatgtcaa acgggcgctc
      781 gccgccatct cgtaaagctc gcgcggcccc tcggtggcgg accagttgac acagatatcg
      841 acgatttcgc cgagcggtcg gtgtaacgcc tcgataagcc ccggcagttc atctgatagt
      901 gacgctgtgt agggccacca cgctccatcg atgtcggccc ctaactgagg ggcaagcgcg
      961 agcctgacgg gccgggccag gcgccgcggc cctgccatgc cgttcacgag gacttctgcg
     1021 catcacgagg caaatcgttt cgaggtcggt cggagaaggc gggaggctgc accggttcgt
     1081 tggaagcatc tctgttcggg acaacttctg gctcatcggg atgcgagaac aagtcggacg
     1141 aattcataac gaagtccttt aatcaagttc aacccgcagc cggatgttcg gggcggacgg
     1201 cggtatcgat tcgtcttgac gaggcaaaca cgaagcggcc tacacaccaa cttacaccct
     1261 tggatatgtc atgtgttcgc ggtcgggtgc cagcgggata gctgccaggc cgactgcgaa
     1321 ggccgtcaag tgccccagct gtgtgaacgt cggcctggcc gccgcattgc cggccaacgc
     1381 caccaacacc gcgacctggc atcgagaccg ccacggccgc ggcgcatatc ccgataacgc
     1441 acccgccacc ccgaaaacga aataactcac cccgacgtcg cgggcgttca ccagccacct
     1501 cggcgccttc ttctttgtga tcaacaaccg cagatagccc tggccgacaa aggtggcgat
     1561 cacgtgcgcc gcgatcccga cgagaagcca acgcctccaa cgtaatcgac gctcggccgg
     1621 agcgaccacc gcgacgaaca cgggcacgtt cggccaccat ttgcgctcgt cgagccagaa
     1681 caggctggtc gctaggaccc tcaaaggttg gctgcggagc cagcgcaggt tggtggagtg
     1741 gtcgtgctga agttgttgcg atccgcctgg cccagccgac cgctgaacgc gcgtggtgac
     1801 gagaagcacc accagccaca ggaacgtgag cggagcgctg gccacactat ggagtacgcc
     1861 gtccagcctc gcagccctca tggagccaac ctagtcagat cgacaggctc tgcttcagat
     1921 tgcactcagg gtcgtgatcc acgtgccgcc gcaaccctca gtgcaattcg gcaatggcag
     1981 cgacgacaac taccgaaccg ccccgttgat caccggcttg tcgatcatcc ctttttcgac
     2041 accccacatc gtggcgatcg tcttgtactc cccggtctgc atcagatgct ccagcgcccg
     2101 ctgcagcgct tccgccaacc cggagccttt ggccaccggc cagccgtacg gcgcggtgtc
     2161 gaagatctcc cccgccggcg ccaactgtcc gccagagagc ttgaccgcga atcccgtcac
     2221 cggtgaatcc gccgccatcg cctcgacctc gcccgcgatc aatgccgccg tcagatcgtc
     2281 ctgccgggtg tagaccacct tgtcgatcgg cgaaaccccc gccgccacac actgatcgct
     2341 tttcgccggg acctcttccg tctcctggat cgacgcgtac gtcacaccaa tcttcaagcc
     2401 gcacgcggcc tccggatcca ccgtcgaccc cgcccgctgc gcccacaagg tccccgcctg
     2461 aaagtacgtc acgaagtcga cgtccgcctc gcgctctttg gtgtcggtga acgacgacat
     2521 ccccgcattg aagctgccgc tcgacaccga cggcatgatg ctctcgaacg ccgtctcgcg
     2581 gtagtccggc gtgagcccca gcgtcttcgc caccgcattc atcaggtcca catcgaagcc
     2641 gacgatctca cccgatgagt tcttgaattc gtttggcgca taagggacat tgacgccgat
     2701 caccaatcgg cccgacttcc tgatgtcctc cggcaccgtc gccgcgatct ccgggaccgc
     2761 gcccgtcacc ggcgccggcg tcgccgaact ctccgccacg ctggccggcc gcgtatcccc
     2821 accgcgcgat ccccgccact gcaacacccc gaacgtgccg accgccgcca ccaacaccac
     2881 cgcagccgca atcgccatcg ctcgcttcga cactccttgt gaccgctgcc gagaatgctt
     2941 gcccgagcga cccgtcgtac gcaccggcgt ctcctgcgca cgccgcgcgg cagcggccaa
     3001 ctccccggcc gtctgatacc gcttcgccgg cttcttcgcc atgcccttgg cgatgacgtc
     3061 gtcgaacgcc gacaacttcg ggtcgatggc cgatggccgc ggcggcggcg acaccatatg
     3121 tcccttgatc tgctgttcgt agctctccgc cggatacggc cgcgacccgg tgatgcactc
     3181 gtagagcaca cacgtcaacg catagatgtc tgcgcgcgga tcgatgtccg caccctcgaa
     3241 ccgctccggc gccatgtacg ccaacgtgcc cagcgtgttg cccgccgtcg tcaaaccctt
     3301 ctcaccggcc gtgcgggcca gcccgaagtc gatcaggtac acgaagtcgc ggtcggtgat
     3361 caggatgttc gacggcttga cgtcgcggtg gatcaggccc gctgtgtgcg cggcatccaa
     3421 cgcgttcgcg acctgcgtca ccacagtgac cgcaaacgcc gccccgagcg gtttgccctc
     3481 ggcctcctcc agcatggtcc cgaggttgcg gccctcgatc agccgcatgt ccagatagag
     3541 ccggccgtcg atctcgccgt agccgtggat cggcaccaca tgcgggtcgt tgagccccgc
     3601 cgcggcctgc gattcccgcc ggaaccgctg ctggaacgtg acatcctcgg cgagatggtg
     3661 cggcagcacc ttgagcgcga caatccggtc ggtcttggtg tcgtaggccc ggtagacctc
     3721 acccataccg ccccggccga tcagcttttg cagctgatag tgcccgaatg gtgtcgcatc
     3781 caccgttaac tccctggtcg ccgaccccgc atcagcgtgc cgtatgaagc tacatcacgt
     3841 ttgccaccgt gtcgtggcgc tcgtcatatc acggccaatt cgaagttagt cgacgctaag
     3901 atgaggatgt ccgtgcctca tcgcggcggc aagaggctga gcggcccgtc gtgtttgact
     3961 tttccttact ggtcaatcca cacctggtgc gcgagtgacc aaggagggtg tcatgcagtg
     4021 gttgacggtg gcctggtggc gctggcgcct ggcgcaagtc cgtgggcgca atccgctggt
     4081 gcggaggagt gatcgcatcg acgtcgccgt cacggcgctg gccgtcgtag tctcgctcgc
     4141 cgtcattcct ttcgcgctcg ccatcggcac cgccgtccac gacgaaaacg cccgaaccta
     4201 cgccgcacag caacacgacc gccacaaggt ggtcgcgaca gcgaccgcgg acagcaccgg
     4261 cggcgctggc aacccgaagg gacccgtcgc cgtcgtttcg gcccggtggc aggtcggcga
     4321 tgtcgagaac gccggcgcct tcacgtggga ctcgccggtc aaagacggct cggacatgga
     4381 catttgggtg gaccgcaacg gccgtcaggt cggcccgccc gcagcgtcct ggcaggcagg
     4441 tgtcgacgcc gccgttgctg cggtggggat gtggcttgcg gtgaccgccg cggccgcgct
     4501 gctcgttgcc gcagttcggc tggggctgcg ccgcgcccgc tacgccggtt ggaaccgcga
     4561 catggtcagc cttgtccacg acaacggagg cagaaccaac cgtcgccggt gattccggcc
     4621 ccagcggtga ctgcatagcc ctaccgtgag cacatggcag aacaacccgc ggttttcgtc
     4681 tcgcacccgc cggagcggct tctccgcgcc gtcaacccgg tcatgcggtt cctgctcggc
     4741 actccgctcg cgggtcccgc gcgcaagcag atgatggtgg tcagcttcac gggacgcaag
     4801 agcgggcgtc agtactcaat cccgttgagc gcccatgtga ttgacaacga gctctacgcc
     4861 ctcacgagcg ctccctggaa gaacaacttc cgcgacggcg ccgacgccga ggtcctcaac
     4921 gacggcaagc ggacgccgat gcgcggcgaa ctcgtcaccg accccgcgat agtcgccgat
     4981 ctctcccatc gctgcgccga gtcctacggc gtgaaacgcg cccagcgcat gatggggctg
     5041 ggcttccgcg atcagcgcat tcccacggtc gacgagttca aggaggcggc cgtacgagaa
     5101 cgcatcgtcg ccgtcaagtt caccccgacc acgtaatggc agagcgcaac gtccttggcg
     5161 gaccgctgga accgtgcggc accgagccac tcaccggctt ctaccgcgac ggctgctgct
     5221 cgacggggca ggaggacatc ggcttgcaca ccatctgcgc cgtcgtgacc gccgagtttc
     5281 tcgagcatca gagctcgatc ggcaacgacc tgtcgacgcc gcgacccgag ttccgattcc
     5341 cggggctcac acccggtgac cgctggtgcg tcacggctgc gaactggttt cgggcttacc
     5401 aggctggctg cgccgctccg gtcgtgctcg cgtccaccca cgagcgcacc cttgacctgg
     5461 tgtcgttgga tgcgttgcag aagcacgccg tcgacgtgcc cgacgatctc gagggcctgt
     5521 agggcacttc ttgcggcagc gaaaattagt gcgtcaaatt tgccacggcg cggtgcttag
     5581 cagcgctacg ctgccgcgat ggacataaac cgacggagtt tcatcggcct caccggcgca
     5641 gccggacttg ctgtactgac cgcacagggt tcccaattag ccagtgccgc accggcttct
     5701 ggacccgtca cgatcgatca cgccttcggc cagaccacca tcccggcgcc gccgaaacgt
     5761 gtcgtcagcg ccgggttcac cgagcaggat gacttgctgg ccctcggcgt cgtcccgatc
     5821 gccaccacgg catggtgggg cgatgagccg ttcgaggtgt ggccctgggc gcggcccaag
     5881 ctgggtaacg cgcaacccac cgtgctcagc atctactccg acgcaggttt cgagttcgac
     5941 cgcatcgctg ggctgaatcc cgatctgatc gtcgccacca atgcaggcgt cgatcaagac
     6001 aagtacaacc gcctgtcggc aattgcgccg acaatcccgc aatcggcggg tcaaccgccg
     6061 ttcttcgaac cgtggcaggt gcaggcgaac accatcggcc aagcggtctt caaggccggc
     6121 gagatggcgt cgctggtggc tgcagcggaa agaaaattga ccgatgtggc gcgggcgaat
     6181 ccccagttcg ccggcaagac agtgcaaatg atgcagggca cgttctttga cgacggcgcg
     6241 tgggcaacgc tgcccggctg gcgcacgaac tttctcactg acatgggcct ggtggttcct
     6301 gacaccttgc gcgccttcac attcgacgac caccgtgcgt tcattccgcg cgatcaggtc
     6361 gccgcagctc tcggcggcgt cgacgtcttg atctggacaa cccaaagtcc cgaggaccag
     6421 gcggggttgc tcgccgaacc gtcggtgcag cagttgaatg ccgcctcgcc gaatcgcagc
     6481 gtctacacgg gtcggaattt ggcggcggcc attgcgttct cgtcgccact gagcctgcca
     6541 gtggtggccg accagttgcc gcctctgctc aaaaccgccg tcatcgccgc ctaggcgaac
     6601 agtcgagtct tcggcgacct gtaggaattg accctgcgcg cacaccgtcc gcggctttga
     6661 cgagaatccg agcgtggaca acctcgccac cgccgcacag agccagctgg accagtggca
     6721 gctgcgccgc gacggggacc cgatccaggg cgcagactcg ctggtcctgc cagtgcggac
     6781 gtcgcacgga gcgcatgcca tcctgaagat gagccagtcc gagcacgaac acctggtgct
     6841 gcgacggtgg ggcggtagcg gcgcggtccg gctgctcagc gccgacccgc cgcaccgtgc
     6901 gcttctgctg gagcggctgc gtccggagag cctcgacacg ctgtcggacg tcgacgcatg
     6961 cgaggtcgtc gccggcctgt accgccgcct tcacgtccct gctctgcccc aattgcacac
     7021 gctcacttcg tatctcgagc agtggacacg cgacttcgag gtgcttccgc gtagcgcgcc
     7081 gatcccgcac cggctcgtcg agcaagcgat cgcgctgggc cgggacctgg cggccgaacc
     7141 gggcgccagc gtcgttcacg gcaacctcca ctacgagaac gtgctggcgg ccgtccgtga
     7201 gccgtggctc gccatcgcgc cccggcccgt gaacggcgac ccgcactacg agatcgcacc
     7261 gatgttgtgg acccgctggg acgagctctc cggcaacatc cgcgatggtg tccggcgccg
     7321 cttttacacg ctggtcgacg cggccggctt cgatgaggag cgggcccgcg cctgggtcgt
     7381 cgtccgagtg gtccagcagg cgatccgcga cccgatgagc ctgactaagt acgtcgcgct
     7441 cgccaaggcc gtgcaggact gagcgacaac ggactttgct acagggccgt caataactgc
     7501 gcttggccgt tgcgacacgt tcccttatgc gccaacagta ttgggtgggc ggatgggcct
     7561 ggcgtacgct cgccacaggt gttgaagcag ccgacacttt gaatccaata cccctattcg
     7621 agcgtcaaaa cagggatgca cagtggcaga aacgcacatc aaagcggctc agtcgaaatg
     7681 gctggccaag tccgcagcgc agacacgcgg atcggataag aaagaagtcc aattccacta
     7741 cgacatctcg aacgagttct tcaagctgtg gcaggacccg acccagacct atagctgtgc
     7801 ctacttcgag cgcgacgaca tgacgctcga ggaagcccag atggccaagg tcgacctctc
     7861 actgggcaag ctgggcctgc aacctgggat gacgctgctc gacatcggct gcggatgggg
     7921 ctccacaatc atgcgtgccg tcgacaaata cgacgtcaac gtcatcggcc tgaccctgtc
     7981 cgagaaccag aagcagcaca tcgaggatta ctggttcgcg aattccacga gcaagcgcca
     8041 catggaagtg cgcctgcagc cgtgggagga attcgacggc aaagtcgacc gggtcgtgtc
     8101 gatcggcgca ttcgagcact tcggcttcaa caaatacgac gactacttca agaagaccta
     8161 cagctggatg cccgacgacg gcgtgatgct gctacacacg atcatcatcc cgagcgatga
     8221 cgagatcaaa gcgaagaaac tgccgctgac catgtcgaac gtgcgcttca tcaagttcat
     8281 catggacgag atctaccccg gcggccggct accgctggcc gcgcaggtcg aggagcacgc
     8341 cgtcaaggcc ggctacaccg tcacccgcaa acagaaactg cagccgcact acgcccgcac
     8401 gctggacacc tgggcagcaa accttgagtc caagaaagac gaagccatcg cgatcacctc
     8461 cgaagaggtc tacgaacggt tccgcaagta cctgaccgga tgtgccgatc tgttccgaaa
     8521 cggctacacc gatgtttgcc agttcacttg cgagaagcgt tgattcagcc aaagagcgaa
     8581 gcgtgaggta gaaaatagga ggggtcaggt ccgtcgggat cgcaatgcga tctgtccgga
     8641 ggaggtcgat aacaagtggc tcagagcaaa gtgggctcaa cgaggatgcg tccgcacttc
     8701 gaggaaatcc aggcccatta cgacctatcc gacgatttct ttgggacgtt ccaggacccg
     8761 tcgcggacat acagctgtgc gttctacgag cgcgaggata tgtctctcga agaagcgcag
     8821 ctggccaaga ttgacctggc cctcgacaag ctggaactgc ggcccgggat gacactgctc
     8881 gacgtcggct gcggctgggg ttcggtgatg aagcgggcag tcgagaaata cgacgtcaac
     8941 gtgatcggcc tgacgttgag caagaaccag gccgtctact gcgaacatct tctcgaggaa
     9001 atcgagtccg atcggtcgcg acaagtgttt ctgcgcggct gggaggagtt cgaccagccc
     9061 gtcgaccgca tcgtcagcat cgaggcgttc gaagccttcc cgaaggagcg ctatgcggcc
     9121 ttcttcgaga cgtgccaccg catcctgccg accgacggcc ggatggtgct gcagacgatc
     9181 ttggggcatc ccctgaagcg ctggcccgac atgggcattc cgatcaccat gaccgacctg
     9241 aagttcatgc gcttcatcgc caaggagatc ttccctggcg gcgccatccc ctgcgatgag
     9301 gacattgcga attactccgg cgctgcaggg ttttcgcttg ttcagacgca gtacctcaac
     9361 gagcactatg tgcggacgct tgaaacctgg gcgctgaatc tcgaagccgc ccaggacgaa
     9421 gcggtcgtcg cgacctccga agaggtctac cagcgctaca tgcgctacct gaccgggtgc
     9481 gccgacttct tccagcgcgg catctgcgaa ctgggccagt tcaccctgac gaagggatag
     9541 cgctcaagcg gcgccgaaga ccacggcgac gttgttgccg ccgaggccga atgagttggt
     9601 gaccgcgtag cggaaatcgg cgcgccgggg acttccagcg acgacgtcga gatcgatttc
     9661 gggatcgagg tctttcagat tgagggtggg cggcaccaca ccgtcgcgca gcgcctgcac
     9721 cgccagcacc gactcgacgg ctccggccgc gcccaacgga tgccccagcg ccgccttggg
     9781 cgcgtagacc gcgggcgtgt gatcgccgaa cgcgtacttg atg
//
LOCUS       DASRJA010000253         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2025261,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000253 DASRJA010000000
VERSION     DASRJA010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025261"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFC01_11385"
     CDS             complement(<1..469)
                     /locus_tag="VFC01_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80274.1"
                     /translation="MTADLNEFRASVRTWCEEHVPADWRRAQTGVGDEEFVRFQKAWF
                     AELHSAGYAVPHWPHEWGGGLSVAEQIVLYQELAAHDAPRLVLAFVGIHHAAATLLAA
                     GTDEQRQRHLPAILDGTIWVQGFSEPEAGSDLASLRTSARRVGDSYVVNGQKLW"
     gene            complement(466..1248)
                     /locus_tag="VFC01_11390"
     CDS             complement(466..1248)
                     /locus_tag="VFC01_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN80275.1"
                     /translation="MKYDLPDELTVEADGPVRTVLMNRADELNCVNENLHWALANVWR
                     QLAADKGAKVVILSGAGRAFCAGGDLNWITSFLDDPVARDESIREGAQIIEEMLRFPL
                     PVIAAVNGPAVGLGCSVAVLSDIVLISENAYLADPHVAVGLVAGDGGAAFWPLLTPIM
                     RSREYLFTGDRIPAATAVELGFASRTVSPDELMTEAQRLAERLAAQPAEAVRGTKRVV
                     NMYLSQALAGPLQAGFSAEVATMQTDEHRQRLMDFQRKSKRS"
     gene            complement(1245..2162)
                     /locus_tag="VFC01_11395"
     CDS             complement(1245..2162)
                     /locus_tag="VFC01_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80276.1"
                     /translation="MILDLSDDAKEYGKQALRAFEAAGGDQLVQQAEAKPDLRESLAG
                     PALNELGAWELDPRIDRDGLEAAAALCRSAGYWALPYPVAERLAKPTDLDVDGLIVVA
                     ENRPSGVVAGLEADWAAVSLDGKRSRVVGQGAAGPGFVAELELSGIDENGAGDLALGL
                     VLPCWTLLGMLDRAMELTISHVSLRKQFGQTLSSFQGVQFQLTDAEVERSGVEILAKY
                     ALWSIGSGRPEGLDDALALRISAIEAADVVFRVCHQLHGAVGFCDETTLSWLSRYSQP
                     LRRLPLGLSATRDVLTHRVGGTGLTGLFT"
     gene            complement(2159..3310)
                     /locus_tag="VFC01_11400"
     CDS             complement(2159..3310)
                     /locus_tag="VFC01_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80277.1"
                     /translation="MEFEMGQKAAELRNELRELVKAHVPEHYLGAFTDNPADLETAQQ
                     FCRTLADRNLLCLAWPEEFGGGGASVWEQTVVREEMWAHHEPRGAQYMGVNWVGPIIM
                     RHGTEEQQRKHLPPIARGEVIWCQGFSEPEAGSDLASLRTAARRDGDGWLVSGQKIWT
                     SYATMAQWCFLLARTSKGEKKQQGLTIFLVPMDDPAIQVRPIRCMMGPHHLNEVFFDD
                     LRVTEADVLGTVDAGWSIVQDVMSFERVGIARYARCERLLAAAPTVLGERWHDLPAEL
                     RARWVRMLTHCRRARLMAYRVVDLHSSGRIQPGDAAAYRIAVTKLDQDSAEVLMDIAA
                     EVSHDDRRAKWFLGEVEDHWKYSQASTVSSGSIEMQRILLSRALLAGAK"
BASE COUNT          570 a         1165 c         1066 g          556 t
ORIGIN      
        1 cccatagttt ctggccgttg acgacgtagg agtcgccgac gcgccgggcc gacgtccgca
       61 agctggcgag gtcggatccg gcctccggtt cggagaagcc ctggacccag atcgtcccgt
      121 cgagtatcgc cggcaggtgt cgctgacgct gctcgtcggt gccggctgcg agcagggttg
      181 cggcggcgtg gtggatgccg acgaaagcca gcaccagcct cggcgcgtcg tgggccgcca
      241 gctcttgata cagcacgatt tgttcggcca ccgacagccc gccgccccat tcgtgcggcc
      301 agtggggcac cgcgtatccg gcgctgtgca gttcggcgaa ccaggccttc tggaatcgga
      361 cgaactcctc gtcgccgaca ccggtctgag cccgccgcca gtctgccggg acatgttctt
      421 cgcaccaggt tcggacactg gcccggaact cgttgagatc cgcggtcatg agcgcttcga
      481 ctttcgctga aaatccatca gccgctgccg atgctcgtcg gtctgcatgg tggcgacctc
      541 ggcggagaag cctgcctgca gcgggccggc cagcgcctgc gacaggtaca tgttgacgac
      601 gcgcttggtg cccctcaccg cctcggcggg ctgcgcggcg agacgctcgg cgagacgctg
      661 cgcctcggtc atcagttcgt cgggcgagac tgtccggctg gcgaagccga gttcgaccgc
      721 ggtggcggcg ggaatgcggt cgccggtgaa caggtactcg cgtgagcgca tgatcggcgt
      781 caacagcggc cagaacgcgg cgccgccgtc gccggcaacc aaccccacgg caacgtgtgg
      841 atcggccagg taggcgttct ccgagatgag cacgatgtcg ctcaacaccg ccacgctgca
      901 gcccagcccg accgccggtc cgttgaccgc cgcgatcacg ggtaggggga agcgcagcat
      961 ctcctcgatg atctgggcgc cttcccgaat actctcgtcg cgagcgacgg gatcgtcgag
     1021 aaacgaagtg atccagttca aatcaccgcc agcgcagaac gcacgacccg ctccggacag
     1081 gatgacgacc ttggcgcctt tgtcggctgc cagctgccgc cacacgttgg ccagtgccca
     1141 gtgcaggttc tcgttgacac agttcaattc gtcagcgcgg ttcatcagga cggtgcgcac
     1201 cggaccgtcg gcctccacgg tcagctcgtc cggcaagtcg tacttcatgt gaagaggccc
     1261 gtcagcccgg tgccgccgac gcggtgcgtc agcacgtcac gagtcgccga caaccccagc
     1321 ggcaaccggc gcagtggctg gctatagcgg gacagccacg acagggtggt ctcgtcgcag
     1381 aatccgacgg caccgtgcaa ttggtggcag acccggaaca cgacgtcggc ggcctcgatc
     1441 gccgatatcc gcagtgccag agcgtcatcg agcccctcgg gccgaccact gccgatgctc
     1501 cacagcgcgt acttggcgag gatctccacg ccgctgcgtt cgacctcggc gtcggtcagc
     1561 tggaactgca caccttggaa agacgacaag gtctggccga actgcttgcg taggctgaca
     1621 tgcgagatgg tcagctccat ggcgcggtcg agcatgccga gcagcgtcca gcacggcagc
     1681 acgaggccaa gcgccaggtc gcctgcaccg ttctcgtcga tgcccgagag ctcgagctcg
     1741 gccacgaaac ccggacccgc ggcgccctgc ccgaccaccc tgcttcgctt accgtccaaa
     1801 gagactgcgg cccaatccgc ttcgagtccg gccactacac cggacggacg gttctccgcc
     1861 acgacgatca agccgtcgac atccaggtcg gtcggtttgg caaggcgctc ggccaccggg
     1921 tacggcagcg cccagtaccc tgcgctgcgg cacagggctg cggcggcctc gagcccgtcg
     1981 cggtcgatgc gcgggtcgag ctcccacgcg ccgagctcgt tcaacgctgg accagcaagc
     2041 gattcgcgca ggtctggctt ggcctcggcc tgctgcacca gctgatcccc acccgcggct
     2101 tcgaaggccc gaagtgcttg cttgccgtac tctttggcgt cgtcgctgag atcgagaatc
     2161 atttcgcgcc cgccaacaag gcccgcgaca gcaggatgcg ctgcatctcg atgctgccgg
     2221 acgacacggt cgaggcttgt gaatacttcc agtggtcctc gacctcacct aggaaccact
     2281 tcgcacggcg gtcgtcgtgc gaaacctcgg ccgctatgtc catcaggacc tcggcgctgt
     2341 cctggtccag cttggtgacc gcaatgcgat acgcggcggc gtcgccgggc tggatgcgcc
     2401 cgctgctgtg caagtcgacc acgcgatacg ccatcaaccg ggcgcgacgg caatgcgtca
     2461 gcatgcgaac ccagcgcgcc cgcaactctg caggcaggtc gtgccagcgc tcgccgagca
     2521 ccgtcggcgc ggcggccagc aatcgctcgc agcgggcgta gcgtgcgatg ccgacgcgct
     2581 cgaacgacat cacgtcctgc acgatcgacc agcccgcatc gacggtgccg agcacatcgg
     2641 cctcggtgac gcgcaggtcg tcgaaaaaca cctcgttcaa atggtgtgga cccatcatgc
     2701 agcggatcgg tcgcacctgt atcgccggat catccatcgg cacaaggaaa atcgtcaggc
     2761 cctgttgctt cttctccccc ttggaggtgc gggcgagcag gaagcaccac tgcgccatgg
     2821 tggcgtaaga cgtccagatc ttctgaccgc tgaccagcca gccgtcgccg tcacgtcgcg
     2881 ccgcagtgcg tagcgacgcc aggtccgaac ctgcctccgg ttcggaaaag ccctgacacc
     2941 agatcacttc gccgcgggcg atcggcggca ggtgtttgcg ctgctgctct tcggtgccgt
     3001 ggcgcatgat gatgggcccg acccagttga cccccatgta ctgggcgcca cggggctcgt
     3061 ggtgcgccca catctcttcg cgcacgacgg tctgctccca caccgacgcg ccgccgcccc
     3121 cgaattcctc cggccacgcc aagcacagca ggttgcggtc ggccagggtg cggcagaact
     3181 gctgggcggt ctcgaggtca gcgggattat ccgtgaaagc acccaaatag tgttcgggca
     3241 catgggcttt cacgagttcg cgcagctcgt tgcgaagctc cgcggctttc tgccccattt
     3301 cgaattccat acttacaccg actcggcagc ttccacgttg gccagaccaa gctcgcc
//
LOCUS       DASRJA010000254         8665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_516023,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000254 DASRJA010000000
VERSION     DASRJA010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8665
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516023"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFC01_11405"
     CDS             <1..627
                     /locus_tag="VFC01_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HZN80278.1"
                     /translation="ISVDYRLAPEDPYPAGLDDAAAALAWVAENGPNLGIDTTRLAVA
                     GSSAGAALATLLAHRAAVGSAPPIVFQLLHQPVLDDRPTPSKDEFDATPGFDGPAVEQ
                     MWRHYRSGDSVPVDAVPARTDELGGVASTLITCSELDPLRDEAVDYALRLMWAGVGTE
                     LHVILGTCHGFDSLLPEWEISQRLFELQGAALRRALHHAASSRATAAW"
     gene            complement(700..2832)
                     /locus_tag="VFC01_11410"
     CDS             complement(700..2832)
                     /locus_tag="VFC01_11410"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZN80279.1"
                     /translation="MRVGAVTAHGAAESLIAQMTEDELLWLLDGDVPARAMLRLPALI
                     RAGPVTGGAVPRLGIPGIRFSDGPRGVVIGRSTAFPVTIMRAATWDPALEERVGLAMG
                     REGRARGANYSGAVCVNLLRHPAWGRAQECYGEDPVLTGQMGAGLTRGLRRNVMACVK
                     HFALNSIENSRFRVDVIVDEHALHEVYLPHFKTVVDAGADSVMSSYNSVNGAFAGENK
                     VLLNDILRDEWGFEGFVTSDWVGGTHDAVESLTAGMDVEMPLRLLRARELPAALRDGR
                     VSRETVLRSARRILSTCLRHYAERDEAEPSTDLVAGPDHRTLARQVAVMGSVLLKNEA
                     VNGEPMLPLSPSIRRIAVIGRLADNPNTGDHGSSLVHPPSTVSVLQGLRGALPSVDVI
                     HCDGRDLATSARAASDADAVVLVSGLGANDEGERILAERGPGPELFGFPFTLRPVRYV
                     LGKITVKAGELFSQFGRGGDRKSLTLHSQDEALVAAVAAVNPRTAVVLIGGSAILMEE
                     WRDKVPGILIGWYPGMEGGRALADVLTGAAEPGGRLPVAIPLRAEHLPFFDADAPRIV
                     YDAWWGQRKLDRDGNPAAFPLGFGLGYTTFEMKLLDHGAGEVDGVATVRVTNTGTRTG
                     STVVQMYAFDGDSPRPVPQLVGFRRIELAAGQHAAVDVRLDLTPTRERDPHTRTWSRR
                     AGTWRILSAPHSPSTFDGAAPLFDSKDL"
     gene            2945..4090
                     /locus_tag="VFC01_11415"
     CDS             2945..4090
                     /locus_tag="VFC01_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80280.1"
                     /translation="MDFSRVELSDEDQAFQDELRAFLDTIVTDEVIARDRQTGENFDE
                     GVHLALGAKGYLAADFNDEADGGFSRMRRRIWNLEIGRAHTPWFHWGTTAMVARAIVQ
                     FGSPELRDEVLARTLSGHYRLCLGYTEPEGGSDVATCKTRAVRDGDGWIINGSKMFTS
                     NAHNASYVFLVTNTDPDAPKHQSLTMFLVPMDSPGVEIQPLRTVDGDRTNITYYSDVR
                     VDDKYRIGEVNGGWRVLREALNDEHGTVERETDGLDKVAAMTEHVLLLGEAVDRLAAT
                     VSLDDESVKYRLGRSIARMEAAMSTPDMFGRVAIAQTMRDVSPDLMDILGTASSLPAE
                     GSAEYIFRLAGPTGIYGGTLEVFRNMIAQHALGLGRPNYSKPAKRQT"
     gene            complement(4168..5100)
                     /locus_tag="VFC01_11420"
     CDS             complement(4168..5100)
                     /locus_tag="VFC01_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZN80281.1"
                     /translation="MTMPEIGVYLPQMGFNYAELLHRARRCEDLGIDSLWLYDHLYGP
                     GVPDYPSLEAWTLATALLSNTERIQVGHMVLCNQFRHPAVLAKMATTLDQISAGRLQL
                     GIGSGSIEDEHTRVGMEWGTFAQRSARLGETLEILRQAFADERIDFTGEHYTVRDMPI
                     KPGPAQQPRPPIIVGGVGEKYTLPLVARYADVWNVPTYALGELEHKAAVLRSLCEDIG
                     RDPDTIVLSVEAVMALAPDDESLPRVRAFAEKRFGIPAFGLHDGGLIGTAPAIVDRLH
                     KLVELGFGQIVLFTHDRGSDETLDLLASEVIARL"
     gene            complement(5097..5984)
                     /locus_tag="VFC01_11425"
     CDS             complement(5097..5984)
                     /locus_tag="VFC01_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZN80282.1"
                     /translation="MRTTKSTEFAHQKRSAGMGELAVTPVRHGPFPGRLAPDAIADYA
                     VATGDETPAVLGGVAVPAVFPVILVFDAQEAARADLPAPAWERARGGVHGEHDIVLHR
                     PLIVDEPLTTWSQISAVRNSRAGTHLVVHLEQTDADGRIAVEQWWTMVLLGLDGVADL
                     GSPPADHRFPDNVRANPLGATTQHLDDRVAHRYAEVSGDWAAHHFDIEVARAAGFDFV
                     FTHGLCTMAICTHRLLALLGVDDPGRVARVAVRFASPTPLDEELTVNAYGIDQNSFAF
                     EATSRGVATITHGRLELRP"
     gene            6038..6955
                     /locus_tag="VFC01_11430"
     CDS             6038..6955
                     /locus_tag="VFC01_11430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN80283.1"
                     /translation="MKFTFTHPMHSHPYNPELVTGSGIATVAAAAEAAGFHGFGFTDH
                     PAPTQRWLESGGHDALDPFVAMGFAAARTTTLRLIPNIVVLPYRNPFVVAKSGATLDL
                     ISDGRFTLGVGVGYLKREFAALGVDFDERGQLFDEALEVIRGIWTTDDFSYEGRHFTA
                     SGITAHPRPVSRPHPPIWIGGNTAAARRRVTAHGDGWCPFPAPAVLAQTARTAEMSSD
                     TLGEGIDDLRRRLEAAGRDPFAIDISFSNAEGGSPGDDAFNADAYLTGVEKLAALGVT
                     WLQVGLPGDSLAHVLETIERFGKSVIAQV"
     gene            7067..7789
                     /locus_tag="VFC01_11435"
     CDS             7067..7789
                     /locus_tag="VFC01_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020112915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZN80284.1"
                     /translation="MNLADYDALSFDCYGTLIDWETGLSSVLAPWAREAGLDLTDEEL
                     LVAYADNEQRVERENPTALYPTILAESFRRIGASLGAEVSDEWADKLGASVPSWPPFP
                     DSPDALATLADDYKLIIVSNVHREGFAGSNTLLGVTFDKIITAEDVGAYKPAPNHFEA
                     LDAALAELDVPRERLLHVAQSLRHDHVPAKRYGLASVWINRRHDRPGWGATAEPVEEF
                     SYAMEFPSMAAFAAAARESQRS"
     gene            complement(8212..8520)
                     /locus_tag="VFC01_11440"
     CDS             complement(8212..8520)
                     /locus_tag="VFC01_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN80285.1"
                     /translation="MSILSSSPTGIFRRKPVDEVEPANLEEGGGLQRVLGLWQLTAIG
                     IGGIIGAGIFSLAGAVENEKAGPGVLLSFLVAGVASACAALSYAEFAGMIPKADRDQR
                     "
BASE COUNT         1566 a         2766 c         2777 g         1556 t
ORIGIN      
        1 atttcggtcg actaccgact cgcccccgaa gacccctatc cggctgggct tgatgatgcc
       61 gccgccgcgc tggcatgggt ggcggaaaac ggaccgaatc tcggcatcga caccactcgg
      121 ctggcagtcg cgggtagcag cgcaggagca gccctggcga cgctgcttgc tcatcgcgcg
      181 gccgtcgggt cggcgccgcc gattgtgttt cagctgttgc accagccggt gctcgacgac
      241 cgccccacac cctctaagga tgagttcgat gcgacgccgg gattcgatgg tcctgccgtc
      301 gagcagatgt ggcggcacta tcgctcgggt gattccgtcc cggtcgatgc cgtaccagcg
      361 cgtactgacg aattgggcgg tgtggcaagc acgttgatca cgtgctcgga gctcgatcca
      421 ttacgcgacg aagcggtgga ctacgcgctg cgactcatgt gggccggcgt aggcacggag
      481 ctgcacgtga tcctgggcac ctgccacggc ttcgattccc ttctccccga gtgggagatc
      541 agccagcggc tcttcgagct tcagggcgcc gcgctgcggc gggcgctgca tcatgccgct
      601 agttcccgcg cgacagcggc gtggtaggcg gtgagtggct gaatgcggtc tcggaacttg
      661 tgatgacgaa gcccaaggcg ttgccctttc tttcggttat cacaggtctt tagagtcgaa
      721 tagcggtgct gcgccgtcga acgtgcttgg gctgtgtggg gcggacagta tccgccaggt
      781 gccggcacgt cgtgaccatg tgcgtgtgtg ggggtcgcgt tcccgggttg gggtcagatc
      841 gaggcgaacg tcgacggctg catgctgacc cgccgcaagt tcaatgcgcc tgaaacccac
      901 caactgcggt accggccgtg gcgagtcacc gtcaaaggcg tacatctgta cgactgtcga
      961 gcccgtgcgt gtaccggtgt tcgtgacacg aacggtggcc accccgtcca cctcaccagc
     1021 tccgtggtcg agaagtttca tctcaaacgt cgtgtatccc aagccgaatc caagcgggaa
     1081 ggcggccgga ttgccgtctc gatcgagctt gcgctgaccc caccaggcgt cgtagacgat
     1141 cctgggtgca tcggcgtcga agaacggaag atgttccgcg cgaaggggaa tggcaacagg
     1201 cagccgtcca ccgggttcgg cggcgccggt gagaacatcc gcgagcgccc ggccgccttc
     1261 cattcccgga taccaaccga tgaggattcc cggcaccttg tcgcgccact cctccatgag
     1321 gatggcgctt ccgccgatca gcactaccgc tgtccgcgga ttcacggctg ccaccgcagc
     1381 gacgagggct tcgtcctgac tgtgcaacgt gagcgacttg cggtcgccgc cacggccgaa
     1441 ctggctgaac aattcaccgg cctttacggt gatcttgccg agaacgtatc gcacggggcg
     1501 caaggtgaag ggaaaaccga agagctccgg tcccggtccg cgttctgcaa ggatgcgttc
     1561 accctcatcg ttggcaccca aacccgagac aaggacaacg gcgtccgcat cggatgcggc
     1621 tctggcgctg gtcgccagat ctcgtccgtc gcaatggatg acgtcgactg agggtaacgc
     1681 cccgcgcaga ccctgcagaa cagaaacggt cgatggggga tgcacaagag aagaaccgtg
     1741 atcgccggtg ttggggttgt cagcgagtcg gccgatcacg gcgatgcggc ggatggaggg
     1801 agatagcgga agcatcggct caccgttgac cgcctcattc ttgagcagaa cgctgcccat
     1861 cacggcgact tggcgagcca acgtgcggtg gtcgggtcct gcgacgagat cggttgaagg
     1921 ttcggcttca tcacgctctg cgtagtgccg aaggcacgtc gacaggatgc gacgggctga
     1981 gcggagtact gtctcgcgag acaccctgcc gtcacgcaat gccgcaggaa gttcgcgcgc
     2041 acgcagcagg cgcagcggca tctctacgtc catgcctgcc gtgagacttt cgactgcgtc
     2101 gtgcgttccg ccgacccagt cgctggtcac gaagccctca aagccccatt cgtcccgcaa
     2161 gatgtcgttc agaagcacct tgttctcgcc tgcgaaggca ccgttgaccg agttgtagga
     2221 actcatcacc gaatcggcac ctgcgtcgac gaccgtcttg aagtgcggca ggtacacctc
     2281 gtgcagcgca tgctcatcga cgatgacgtc gactctgaag cgtgagttct caattgagtt
     2341 gagtgcgaag tgctttacac atgccatgac gtttcggcgt agtccccggg tcagccccgc
     2401 gcccatctgc ccggtcagca ccggatcctc gccgtagcac tcctgcgctc gcccccacgc
     2461 gggatgacgc aagagattca cgcaaaccgc tcccgaatag ttggcgccgc gggcgcgtcc
     2521 ctctcgtccc atggcgagcc cgacgcgttc ctcgagtgcc gggtcccacg tggcggcccg
     2581 catgatcgtc accgggaacg cggtcgaccg tccgatcacg acaccgcgtg ggccgtcgct
     2641 gaaacgaatg cctggtatgc ccagccgcgg taccgctcca ccggtgaccg gacccgcccg
     2701 gatcagcgcg ggtaaccgca gcatggcgcg cgcgggaacg tccccgtcga gcaaccagag
     2761 cagttcgtcc tcggtcatct gcgcgatgag cgactcagcc gcgccgtgtg cggtgaccgc
     2821 ccctaccctc acctgcctca cacagtcgcc gaatgcgacg cggcctgtcg cattcgaggg
     2881 cgtcatgaga cccgctttac aaaattagtc aagagtgtca cggtggtctg gtggtggccg
     2941 gctgatggac ttctcacgcg tcgagctgtc cgacgaggat caggcattcc aggacgagct
     3001 gcgggcgttc ctggacacta tcgtgaccga cgaggtcatc gcccgagacc gacagacggg
     3061 cgagaatttc gacgagggtg tgcacctcgc gctgggcgcc aagggctatc tggccgccga
     3121 tttcaatgac gaagccgatg gcggtttcag tcggatgcgc cgacgcatct ggaacctcga
     3181 gatcgggcga gcacacacgc cgtggttcca ctggggcacg acggcgatgg tcgcgcgagc
     3241 gatcgtgcag ttcggctcgc ccgagctcag ggacgaggtg ctggcacgca cactgtcggg
     3301 acattaccgg ctatgcctcg ggtacaccga gcctgaaggc ggttccgatg tcgcgacttg
     3361 caagacgcgt gcggtgcgcg acggcgacgg ctggatcatc aacggctcca agatgttcac
     3421 gtccaatgcg cacaacgcgt catatgtctt cctggtcacc aatacggacc cggatgcacc
     3481 gaaacaccag agcctgacaa tgtttctggt accgatggac tcaccgggtg tggagatcca
     3541 accccttcgc accgtcgacg gcgaccgcac caacatcacc tactacagcg atgtgcgcgt
     3601 cgacgacaaa taccgcatcg gtgaggtcaa cggcggctgg agggtgctgc gtgaggcgct
     3661 caacgacgag cacggcacgg tcgagcgaga gaccgatggt ctggacaagg tcgccgcgat
     3721 gaccgagcat gtcctgctgc tcggagaggc cgtcgaccgg cttgccgcaa cggtgtcgct
     3781 tgatgacgag tcagtgaaat accggttggg gcgcagcatc gcccgaatgg aggcggcgat
     3841 gagcacaccg gatatgttcg gccgagttgc aattgcacaa accatgcggg atgtatcgcc
     3901 agatctgatg gacatcctcg gtaccgcttc gagcctgccc gcagagggtt cggccgagta
     3961 catcttccgg ttggccgggc caacgggaat ctacggcggc acactggagg tgttccgcaa
     4021 catgatcgcc cagcacgcac tcggcctcgg ccggccgaac tattcgaaac ccgcgaagcg
     4081 gcagacctga cgacgttttt gcgctgaggg ttgtgatggg acggataccc acagccgtca
     4141 gtgcaaattc gagctctgct gccgccccta caagcgggcg atcacttcag aggcaagaag
     4201 atcgagggtt tcgtcggagc cgcggtcgtg ggtgaacagc acgatctggc cgaaaccgag
     4261 ctcgaccagc ttgtgcaggc gatcgacgat cgcgggcgca gtgccgatca gcccgccgtc
     4321 gtgcagcccg aaagccggta taccgaaccg cttctcggcg aaagcacgga cgcgcggcag
     4381 cgactcgtca tccggggcga gcgccatcac cgcctcgacc gagagcacaa tcgtgtcggg
     4441 atctcgtccg atgtcctcac agagcgaccg cagcaccgcc gctttgtgct cgagttcacc
     4501 caacgcgtac gtcggcacgt tccacacatc ggcgtagcgg gccaccaacg gcagcgtgta
     4561 cttctcgccg accccaccca cgatgatcgg tggccgaggc tgctgggccg gtcccggctt
     4621 tattggcatg tcccgcaccg tgtaatgctc accggtgaaa tcgattcgct catcggcgaa
     4681 cgcctggcgc agaatctcca atgtctcgcc gagccgggcg gatcgctggg cgaatgtgcc
     4741 ccactccatg ccgacgcgcg tgtgctcgtc ttcgatggag ccgctgccga taccgagctg
     4801 caaccgaccg gccgaaatct ggtcgagcgt agtggccatc ttggccagca cggcgggatg
     4861 acgaaactga ttgcacagca ccatatgtcc gacctggatg cgctctgtgt tgctgagcag
     4921 cgcggtcgcc aacgtccacg cttccagtga cgggtagtcc ggcactccgg gcccgtacag
     4981 atggtcgtag agccacagcg agtcgattcc gaggtcctcg catcgtcgcg cgcggtgcag
     5041 cagttctgcg tagttgaagc ccatctgagg caggtagacg ccgatctcgg gcatggtcat
     5101 ggccgaagtt ccaatcttcc gtgagtaatg gttgcgacgc ccctgctggt cgcctcgaat
     5161 gcgaacgaat tctggtcgat gccataggca ttcaccgtca actcctcgtc caacggcgtc
     5221 ggcgacgcga accgcaccgc cacccgcgca acccggccgg gatcgtcgac accgaggagg
     5281 gcgagcagcc ggtgcgtgca gatcgccatg gtgcagaggc catgtgtgaa gacgaaatcg
     5341 aatccggccg cccgcgccac ctcaatgtcg aaatggtgtg ccgcccagtc gccggacacc
     5401 tcggcgtagc gatgggcgac ccgatcatcg aggtgctgag tggtcgcacc gagcggattg
     5461 gcccgtacgt tgtcggggaa gcgatggtcg gcgggcggcg accccaaatc ggcgacgccg
     5521 tccagtccga gcagcaccat cgtccaccac tgctcgacgg cgatgcgtcc gtcggcatcg
     5581 gtctgttcga ggtggacgac gaggtgggtc cccgcccggg agttccgcac cgccgagatc
     5641 tgcgaccagg tagtcagcgg ttcgtcgacg atcagtggtc ggtgcagcac gatgtcgtgt
     5701 tcgccgtgca caccaccgcg agcgcgctcc cacgcgggcg ccggtaggtc ggcccttgct
     5761 gcctcctgtg catcgaagac gaggatcacc ggaaaaacgg cagggacggc cacgccgccc
     5821 agcaccgcgg gggtctcatc cccagtggcc acggcatagt cggcgatggc atcgggcgcg
     5881 aggcgaccgg ggaatgggcc gtgcctgacg ggtgtcaccg ccaactcgcc catgcccgct
     5941 gacctcttct gatgcgcgaa ctctgtactt ttcgtggttc gcatggtaac gacattctca
     6001 gaaattgaga agattgtttc tgaagcgggg cagtttgatg aagttcacgt tcacccatcc
     6061 gatgcacagc catccgtaca acccggagct ggtgaccgga tcgggtatcg cgacggtggc
     6121 cgctgccgcg gaagccgcag gctttcatgg attcgggttc accgatcacc ccgcgcccac
     6181 gcagcgctgg ctggaatcgg gcgggcatga tgcgctcgac ccattcgtgg cgatggggtt
     6241 cgccgcggcc cgcacgacga cgctgcggct gatcccgaat atcgttgtgc tgccgtaccg
     6301 gaatccgttc gtggtggcca agtccggggc aacgctcgat ctgatctccg acgggcgatt
     6361 cacgctcggt gtcggtgtcg gctacctcaa acgagaattc gccgcgctgg gtgtggactt
     6421 cgacgaacgc ggacaattgt ttgacgaagc gctggaagtg atccgcggga tctggactac
     6481 cgacgacttc tcgtatgagg gacggcattt cacggccagc ggcatcacag cccatccacg
     6541 gccggtaagc cgccctcatc cgccgatctg gatcggcggc aacactgccg ccgcgcgccg
     6601 gcgcgtaacc gcgcacggtg atggctggtg cccgtttccg gcacccgctg tcctcgccca
     6661 aacagcgcgc accgcggaga tgagctcaga cacccttggc gaaggcatcg acgatctgcg
     6721 gcgtcgattg gaggctgcgg gtcgggatcc gtttgccatc gacatcagct tctccaatgc
     6781 cgagggtggc agcccaggcg acgacgcgtt caacgccgac gcctacctca ccggagtgga
     6841 aaagcttgcg gcgctgggtg ttacctggct acaggttggc cttccgggcg acagcctcgc
     6901 gcacgtgctg gagaccatcg aacgcttcgg aaagtcggtc atcgctcagg tctgagaccc
     6961 ctgcaggggc tcgagtggct gcgggacggc aaaaatccga tgacaggtgg catcgcctac
     7021 ggcgatgatt gagtaggcag cgatccaact ggcagaggtg taacgagtga atcttgctga
     7081 ctacgacgcg ttgagcttcg actgttacgg aacactcatc gactgggaaa ccggactctc
     7141 gtcggttctg gcgccgtggg cccgcgaggc cggtctcgat ctcaccgacg aggaactgct
     7201 ggttgcctac gcggacaatg agcagcgcgt ggaacgtgag aaccccaccg cgctgtatcc
     7261 gacgatcctc gccgagtcgt ttcgacggat cggcgcgagc ctgggcgcgg aggtgtcaga
     7321 tgaatgggcc gacaagctcg gcgcgtccgt gccgtcgtgg ccgccgttcc ccgattctcc
     7381 cgacgccttg gcgaccttgg ccgatgacta caagctgatc attgtctcca acgtgcaccg
     7441 tgagggattc gcaggaagca acactcttct cggtgtcacc ttcgacaaga tcatcaccgc
     7501 cgaggacgtc ggagcttaca aaccggcccc caatcatttc gaagccctcg acgcggcact
     7561 ggccgagctc gacgtgccgc gagaacgatt actgcacgtg gcgcaaagcc ttcgccacga
     7621 ccatgtaccg gcaaagcggt acgggctcgc ctcggtgtgg atcaatcgcc gccacgaccg
     7681 cccgggttgg ggcgccacag ccgaaccggt tgaggaattc agctatgcga tggagtttcc
     7741 ctccatggcg gcgttcgccg ccgcagcacg agagagtcag cggagctgac cgagtagcgg
     7801 cccaaaacgg gctaggctcg gcatacacgt atggctacgt cgtcttgggc gagggcgtcg
     7861 gctggtgctc ggctgggacc tgctgctgga gtacaccgcc atcgttgccg tggttggcat
     7921 tggccggtta tttcggcttc ctcgtcgaac agatcgggct gaacttgccc gagtggatgc
     7981 tcggggcgcc gggcagcgac cctgaccagc cgggccgcgt ggtcgacctg ttcgcattgc
     8041 tgctgtgcct gttgatcgcc gtcatcctca cccgcggcgt gcgcagcgcc gctcgcgtgg
     8101 agaccctgct cgtctggctc aaggtcggag tcgtcgacaa ctacacgccg ttcctgccgt
     8161 tcggctgggg cggagtgttc gccggcgcat cggtggtgtt cttcgccgtc ttcaacgctg
     8221 atctcggtcg gccttgggga tcattcccgc gaactctgcg tacgacagcg cggcgcacgc
     8281 gctcgcgacg cccgcgacca gaaacgacaa gagaacccct ggcccggcct tctcgttttc
     8341 caccgcgccg gcgagagaga agatgccagc gccgatgatg ccgccgatcc cgatcgcggt
     8401 gagctgccac aaccccagca cgcgctgaag tccgccaccc tcctctagat ttgcaggttc
     8461 gacctcatcc accggcttac gacggaatat gcctgtcggt gaagagctga gaatgctcat
     8521 gcagcatggc ctttcgacta tgaacgccga cagcgttcga tcgatgtgac ctgcctcacg
     8581 cttgcgtttg cggtcgtgtg caccgcggtc atcgtgttgc gctaccggtc gcccgatatc
     8641 gagcgcggct tccgcactcc cggca
//
LOCUS       DASRJA010000255         9123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2680898,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000255 DASRJA010000000
VERSION     DASRJA010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9123
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2680898"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1922)
                     /locus_tag="VFC01_11445"
     CDS             complement(69..1922)
                     /locus_tag="VFC01_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80286.1"
                     /translation="MKPAAPVTSVRIGSPYRGPVCAAMDIGSTRVNWVARAAAALIVV
                     QLVVRAVLAFQGYFYWDDLILTGRAGTQGLLSPTYLFDDHDGHVMPGAFLVAGGITRL
                     APLNWIGPAISLVVLQSLASLALLRALHVVLGWRPVLLLPLTFALFTPLGVPAFAWWA
                     AALNSLPMQAALAWVCADAVLLVRTGQHRYAMTGLLVYFGGLLFFEKAAVIPFVAFTI
                     AALYAHVTSSGSIVDVWRRGVRLWVPALTMTAAWIVVYLVVVDQKRWSFDVAMTWDLL
                     SRSVTHGIVPGLVGGPWDWQRWAPASPWATPPVVVIVVGWMALAIVAAVSFIRKQRIG
                     AVWLVAVGYAVACQIPIYLMRSSRFTALELAQTLRYLPDLVVVLALLLAVGLCAPNRS
                     ESSWLDASQRRTVITVGFTGLFVVSSLYSTATFLTSWRDNPAQPYLQNAQAGLAAAHA
                     GSSAPLLDQEVDPLVLQRVATPENLASHMFALLRDRPEFAGATTDLRMLDVSGHVVDA
                     KVTWVRSIVPGPAPNCGYLVQPDFPVSMPLDGPLLPADWTAEINYLANSDGSLTMSLS
                     EGTETKVPVRPGLNRVFVRLSGAGDAIKVRANTAALSVCIAAGPVGFVAPR"
     gene            1888..2826
                     /locus_tag="VFC01_11450"
     CDS             1888..2826
                     /locus_tag="VFC01_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN80287.1"
                     /translation="MRTLVTGAAGFIGSTLVDRLLADGHTVVGVDDLSSGRPENIIEA
                     ERHDGFEFAKSDIVSADLIGLLADTQPEVIFHLAAQIDVRHSVVDPQFDSMVNVVGTV
                     RLAEAARQAGVRKIVHTSSGGSIYGTPQTFPTPEDIPADPASPYAAGKVAGEVYLNMF
                     RNLYGLECSHIAPGNVYGPRQDPHGEAGVVAIFSQALLSGRPTKIFGDGTDTRDYVFV
                     DDVVDAFVKASADAGGGQRFNIGTGVETSVRQLHSVIAKAAGAADEPEFHPPRLGELK
                     RSSLDNSRAKQVLGWVPQVDLVEGVARTVDYFRQSR"
     gene            complement(2823..3164)
                     /locus_tag="VFC01_11455"
     CDS             complement(2823..3164)
                     /locus_tag="VFC01_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HZN80288.1"
                     /translation="MNWIQALLITSVLALLVYLLRSRRSARSKAWVKVGYVLFVIAGI
                     YAILRPDDTTVVANWLGVARGTDLMEYVLIIAFVFTTLSAYMRFKDLELKYARLARAV
                     ALQTARAPEER"
     gene            complement(3161..3868)
                     /locus_tag="VFC01_11460"
     CDS             complement(3161..3868)
                     /locus_tag="VFC01_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN80289.1"
                     /translation="MFCIDTRYHDVWIVIPAFNEASVIGDVISDVRSVFDHVVCVDDG
                     SRDDTGDVAWRAGAHSVRHPVNLGQGAAIQTGVEYARQQPGAAVFATFDADGQHRVKD
                     VVRMIDRLSTDEVDLVIGTRFADPGVVSHTPLLKRLVLRSAAAASRRSRKLGLTDAHN
                     GLRVFNKKVADGLNLTMSGMSHADEFIALAYENHWRVTEEPVEILYTEYSMSKGQPLL
                     NGVNIIFDGLLRGRMSR"
     gene            complement(3878..5122)
                     /locus_tag="VFC01_11465"
     CDS             complement(3878..5122)
                     /locus_tag="VFC01_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80290.1"
                     /translation="MTEAATPTGPITRASVARVGTATVISALCGYAVLYLAARDLEPA
                     GFSIFGVFWGAFGLVTGAAFGLLQEATREVRSARYAEVVAGPRTHPMRVAAMVGVVSA
                     AVIAASSPLWSAHVFVESRWLSVALLSVGLAGFCLHATLMGMLAGVNRWSQYGSLMVT
                     DTGMRVIVAAATFVVGWGLVGYLWATVAGAVAWLIVLIASPATRAAARLLTPGGTITF
                     LRGASHSIAAAGASAILVMGFPVLLKATSGDLGASGGVVILAVTLTRAPLLVPLTAMQ
                     GNLIAHFVDQPAHRLRALVPPAVVVTGLGLLGVLLAGVFGPWLLREGFGAQYRADGEL
                     LAWLTAAAIAIALLTLTGAAAVAAALHRAYSAGWVSATVASALLLLLPVGLETRTVIA
                     LLCGPLVGIAVHLAALARVSQV"
     gene            complement(5119..6465)
                     /locus_tag="VFC01_11470"
     CDS             complement(5119..6465)
                     /locus_tag="VFC01_11470"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZN80291.1"
                     /translation="MTRSKKAAKKAAPPCIDPYLPANGNFGYRVSRYELDLEYKVTIN
                     RLSGSATITAVTLAALRTFTLDLSDALSVTKVSVNGKRPAQFRSSSGKLHIALHEALP
                     AGAAMTIAVRYGGTPRPIHSYWGEVGFEELTNGVLVAGQPNGAASWFPCDDHPSAKAS
                     FRIRISTESPYYALANGELLSRRARAGMTTWTYEQPEPTSTYLVTLQIGMYDRHRMAK
                     NGVQMHAVLPGRLRHNFDQDFADQPQMMKLFVKLFGPYPLASGYTVVVTDDDLEIPLE
                     AQGISIFGANHCDGQGSAERLIAHELAHQWFGNSVTAKRWRHIWLHEGFACYAEWLWS
                     EHSGGRSADEWAHHYHQRLADSPQDLLLADPGPRDMFDDRVYKRGALTLHVLRRRIGD
                     ESFFALLRDWTTRYRHGTAVTDDFTGLAANFAHESLRPLWDAWLYSLAVPALDGPS"
     gene            complement(6462..>9123)
                     /locus_tag="VFC01_11475"
     CDS             complement(6462..>9123)
                     /locus_tag="VFC01_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Pls/PosA family non-ribosomal peptide
                     synthetase"
                     /protein_id="HZN80292.1"
                     /translation="VSGGAAAVRRTASGTPLLVGYVASADPSFDLAKARVALAETLPA
                     ALVPRLVLVDELPTRTSGKVDRDALPWPPPGESAEEEPDLGGTMGWLAGLWRDILAAP
                     VDGPEADFHALGGGSLSAAQLVAAMRQRYPQVTVADLYDHPRLGSLAGYLDELDPPPT
                     VETRVVKPTPRLTQAAQVVLSLPLATLTGLQWVVWLALLNNVVAQLHPVPWLVPVNWW
                     WIAAGFVLFITPLGRMSIAAVCARILLSGLKPGTYRRGGPVHLRVWLAERLADASGAE
                     NQAGAPWLVYYARMLGNKVGKGVDLHSAPPATGMLRLGHRCSIEPEVDLSGHWIDGDH
                     FHVGPITVGNDATIGARTTLLPGAVVGKNADVAPGSGVVSKVKNGQYWKGSPAVKSGK
                     ARHPWPDHRPRRAPLWVAMYGVTSIVLGGVPLLALGVGLAVIGWGVRDAGSLRDAIEP
                     AALWTPAATLAAVVTYAALTVIGVRVLSMWMPEGYHPVRSRVGWQLWATERLMDAARN
                     YLFPIYASLLTPWWLRVLGAKVGRGTEISTALLVPKFTVVEDGAFLADDTMVASYELG
                     GGWIHVAKATIGKRAFLGNSGITQPGRRVPDDGLVAVLSATPHKAKAGSSWLGSPPVR
                     LRRKPTAADALRTFHPSLRLKILRALVETCRLIPMMVTFAIGVGVLFALQWLATEVGW
                     AWAALASGMALLIAGALAGTIAVVAKWLVVGRIRAIEHPLWSSFVWRNEVSDTFVETV
                     AAPWFARAASGTPVMNLWLRALGAKIGRGVWCETYWLPEADLVTLEKGATVNRGCVVQ
                     THLFHDRVMRMDTVVLEEGSTLGTHCVALPAARIGAGATVGPASLVMRGDEVPPSTRW
                     QGNPIAPWNLFRKKPGDAPKPPKKTEASAA"
BASE COUNT         1719 a         3264 c         2794 g         1346 t
ORIGIN      
        1 cgcgcattcg accacgtcga caagaacgcg ttcagcacac tcacggcacg attatcccaa
       61 cccccggact accgaggcgc cacgaatccg accggacccg ccgcgatgca caccgacaac
      121 gccgccgtgt tcgcccgcac cttgatcgca tcacctgccc ctgacagtct cacgaagacg
      181 cggttcagcc ccggtcgtac cggcaccttc gtctccgtgc cctccgacag cgacatcgtc
      241 agcgacccgt cgctattggc caggtagttg atctccgccg tccaatccgc aggcagcagc
      301 gggccatcca acggcatcga cacggggaag tcgggctgca ccagatagcc gcaattcggc
      361 gccggcccgg gaacgatcga ccgcacccac gtcaccttcg cgtcgacgac atgaccactc
      421 acatccagca tgcgtaaatc tgttgtcgcg cccgcgaatt cgggcctgtc ccgcaatagc
      481 gcaaacatgt gactggccaa gttctccggc gtcgccaccc gctgcaatac caacgggtcg
      541 acctcctggt cgagaagcgg cgcactcgaa cccgcgtgcg ccgcggccag ccccgcctgc
      601 gcgttctgca ggtacggttg cgccgggttg tcacgccaac tcgtcaggaa cgttgcggtc
      661 gaatacaggc tgctcacgac gaaaagccct gtaaaaccga cagttatcac cgttcgcctc
      721 tgcgacgcat ccagccacga agactcggaa cggttcggcg cacacaaccc aaccgccagc
      781 agcagagcca ataccaccac cagatccggc aggtagcgca acgtctgcgc cagctcgagc
      841 gcggtgaacc gcgacgaacg catcaggtaa atcgggatct ggcacgccac cgcgtacccg
      901 acggccacca gccacaccgc cccgatccgc tgtttacgaa tgaacgaaac cgccgccaca
      961 atcgccagcg ccatccagcc cactacgatc accaccaccg gcggtgtcgc ccacggtgac
     1021 gccggcgccc agcgttgcca atcccacggt ccgccaacca aacccggcac gatgccatga
     1081 gtcaccgacc ggctcaacag atcccatgtc atcgccacgt cgaagctcca ccgcttctgg
     1141 tcgaccacca ccagatagac gacgatccat gccgccgtca tcgtcagtgc cggtacccac
     1201 agccgcaccc ctcgccgcca cacgtcgacg atcgaacccg acgatgtgac atgggcgtac
     1261 agcgccgcaa ttgtgaacgc cacaaacggg attaccgccg ccttctcgaa gaacagcagg
     1321 ccaccgaagt acaccagcag gcccgtcatc gcgtaccggt gctggcccgt acgaaccaac
     1381 aacaccgcat cggcacacac ccacgccagc gccgcctgca tcggcagcga gttcagcgcc
     1441 gccgcccacc acgcaaacgc cggcacgccg agcggggtga acaacgcaaa cgtcaacggc
     1501 aagagcagca ccggccgcca gcccagaacg acgtgcagag cgcgcagcag cgccagtgag
     1561 gccaacgact gcagcaccac caaactgatc gccggcccga tccagttcaa cggcgcgagc
     1621 cgggtgatgc cacccgccac cagaaacgcc cccggcatga catgcccgtc gtgatcgtcg
     1681 aacagatacg tcggcgacag caggccctgc gtgcctgccc ggcccgtcag gatcaggtcg
     1741 tcccagtaga aatagccctg aaacgccagc accgcccgga ccaccaactg caccacgatt
     1801 aacgcggcgg cggcgcgggc gacccagttc actcttgttg accctatgtc catcgccgcg
     1861 caaaccggtc ctcggtatgg tgagccgatg cgcacactgg tgacgggggc agccggtttc
     1921 attggatcaa cgctggtaga ccgcctactt gcagacggac acaccgtcgt cggagtcgac
     1981 gatctcagct cgggccgacc cgagaacatc atcgaggctg agcggcatga cgggttcgag
     2041 ttcgcgaagt ccgacatcgt cagcgccgac ctcattggcc tgctcgccga cacccagccc
     2101 gaggtgatat ttcacctggc cgctcagatc gatgtgcgcc actccgttgt cgacccgcag
     2161 ttcgactcga tggtcaatgt cgtcggcacc gtgcggctcg cggaggccgc gcgtcaggcc
     2221 ggggtccgca agatcgtgca cacgtcgtcg ggcgggtcga tctatggcac cccgcagacg
     2281 tttccgacgc ccgaggacat cccagcggac cccgcatctc cgtatgccgc gggaaaggtt
     2341 gcgggggagg tgtatctgaa catgttccgc aacctctatg ggctggagtg ctcccacatc
     2401 gcgcccggca acgtctacgg cccgcgccaa gacccccatg gcgaagcggg cgtggtggca
     2461 attttctcgc aggcactgtt gtcgggccgg cccaccaaga tctttggtga tggcaccgac
     2521 acccgcgatt atgtgttcgt cgacgacgtc gtcgacgcgt tcgtcaaggc ttccgcagac
     2581 gccggtggcg gccagcgttt caacatcggc accggagtgg aaacgtcggt gcgtcaacta
     2641 cattcggtga tcgccaaggc cgccggtgcg gccgacgaac ccgagttcca cccgcctcgg
     2701 cttggagaac tgaagcggtc gagcctggac aacagccggg cgaagcaagt gctcgggtgg
     2761 gtaccgcagg tcgatctcgt cgagggcgtt gcacgaacgg tggactactt ccgccagtcg
     2821 cgctagcgct cctcgggcgc tcgggcggtt tgtaacgcaa ccgcacgcgc cagccgcgcg
     2881 tacttcaact cgaggtcctt gaaccgcatg taggccgata gcgtcgtgaa cacgaatgca
     2941 atgatcagca cgtattccat caggtcggta ccgcgggcca cgccaagcca attcgcgaca
     3001 acggtggtgt cgtcgggacg aaggatcgca tagatgcccg cgatcacgaa cagcacgtag
     3061 cccaccttca cccacgcctt ggaccgcgcg ctacgtcgtg agcgcagcag gtagaccaac
     3121 agcgccagga ccgacgtgat cagaagcgcc tgaatccagt tcatcgcgac atccttcctc
     3181 gcaacaaacc atcaaagatg atgttgacgc cgttcaacag gggttgcccc ttcgacatcg
     3241 agtattcggt gtagagaatc tcgaccggtt cttctgttac ccgccaatgg ttttcataag
     3301 ccagcgcgat gaactcgtcc gcatggctca tgccgctcat ggtgagattc agcccgtcgg
     3361 ccaccttctt gttgaacacc cgaaggccgt tgtgcgcgtc ggtcagaccc agcttgcggc
     3421 tgcggcggct cgccgcggcc gccgagcgca acaccaacct cttcagcagt ggcgtgtggc
     3481 tgacaacgcc ggggtcggcg aaccgggtgc cgatgaccag atcgacctcg tccgtcgaca
     3541 gccggtcgat catccgaact acgtccttga cgcgatgttg accgtcggcg tcgaacgtgg
     3601 cgaagaccgc ggcacccggc tgctggcggg cgtactcgac gccggtctgg atcgccgcgc
     3661 cctggccgag gttgaccgga tgacgcaccg aatgcgcccc agcgcgccac gccacgtcgc
     3721 cggtgtcgtc gcggctgccg tcatccacac agaccacgtg gtcgaaaacg gagcgaacgt
     3781 cagaaatgac gtcgccgatg acgctcgcct cgttgaaggc ggggatgacg atccagacgt
     3841 cgtggtagcg agtgtcgatg cagaacaaaa tacaggctca aacctgcgac acccttgcta
     3901 gcgcggccag gtggaccgcg attccgacca acggcccgca cagcagcgcg atcactgtgc
     3961 gcgtctccaa ccccaccggc aacaacagca acagcgccga cgcgaccgtc gccgacaccc
     4021 aacccgccga atacgcgcgg tgcagggcgg ccgcgaccgc ggcagccccc gtcaacgtca
     4081 gcaacgcgat cgcgatcgcc gccgcggtga gccaggccag cagctcgccg tcggcccgat
     4141 attgcgcgcc gaacccctcc cgcagcagcc atggcccgaa gacgccagcg agcagcacac
     4201 ccagcagccc gaggcctgtc accacgaccg caggcggcac cagagcccgc agccggtgcg
     4261 cgggctgatc cacgaaatgc gcgatcagat tgccctgcat ggcggtcagc ggcaccagca
     4321 gcggtgccct ggtcagcgtc acggccagga tcacgacgcc gccggaggcg cccagatcac
     4381 ccgatgtggc cttgagcagc accgggaagc ccattaccag gatcgcgctg gcgcccgcgg
     4441 cggctatcga gtgcgacgcg ccgcgcagga aggtgatcgt gccacccggc gtcagcagcc
     4501 gggccgccgc gcgcgtcgcc ggagacgcga tgagcacgat cagccacgca accgcaccag
     4561 cgaccgtcgc ccacaggtat ccgaccagcc cccagccgac cacgaacgtc gccgctgcca
     4621 cgatcacccg catgcccgtg tcggtcacca tcaacgaccc gtactggctc cagcggttca
     4681 ccccggccag cattcccatc agcgttgcgt gcagacagaa ccccgccaac cccacgctca
     4741 gcagtgcgac gctcaaccac ctcgactcca caaacacatg cgcggaccac aacggcgagc
     4801 tcgccgcgat caccgccgca gacaccacgc cgaccatggc cgccacccgc attggatgcg
     4861 tgcgcggtcc cgcgacgacc tcggcatacc gcgccgagcg cacctcacgg gtggcctcct
     4921 gcagcagtcc gaacgccgcg ccggtgacca acccgaacgc accccagaac acaccgaaga
     4981 tcgagaagcc cgcgggctcg agatcccggg cggccagata tagcaccgcg tagccgcata
     5041 gcgctgagat cacggtcgcc gtgccgacac gggccacgct ggcgcgcgtg atcgggccgg
     5101 tcggtgtcgc cgcctccgtc acgacgggcc gtccaaggcg ggcaccgcca acgaatacag
     5161 ccatgcgtcc cacaatggcc gcagcgactc gtgtgcgaaa ttggctgcca gaccggtgaa
     5221 gtcgtcggtg accgcggtgc cgtgtcggta gcgtgtcgtc caatcccgca gcagcgcaaa
     5281 gaaactctcg tcgccgatgc gccgccgcag cacatgcagc gtcagcgcgc cacgcttgta
     5341 cacgcggtcg tcgaacatgt cgcgcggccc cggatcggcc aacagcagat cctgtggcga
     5401 atcggcgagc cgctggtggt agtggtgtgc ccattcgtct gcgctgcgac cgccggagtg
     5461 ttcggaccac agccattcgg cgtagcaggc gaagccctcg tgcagccaga tatgccgcca
     5521 tcgtttggcg gtgaccgaat tgccgaacca ctggtgcgcg agttcatggg cgatcagccg
     5581 ttctgcgctg ccctggccat cgcaatggtt cgcaccgaag atcgaaatac cctgtgcctc
     5641 aaggggaatc tccaggtcgt cgtcggttac caccaccgtg tagccgctcg ccagcgggta
     5701 tggtccgaac agcttgacga acaacttcat catctgcggc tgatcggcga aatcctgatc
     5761 gaagttgtga cgcaatcggc ccggcagcac ggcgtgcatc tggacgccgt tcttggccat
     5821 gcgatgacgg tcgtacatgc cgatctgcag cgtgaccaga tacgtcgacg tcggctccgg
     5881 ctgttcgtac gtccaggtcg tcatgcccgc cctggcgcgc cgcgacagca gttcaccgtt
     5941 ggccagcgcg tagtacgggc tctcggtgct gatccggatg cggaagctgg ccttggcgct
     6001 ggggtggtcg tcgcacggga accacgacgc cgcgccgttg ggttggcccg ccacgagcac
     6061 accgttggtc agttcctcga aaccgacttc gccccaatag gaatgaattg gtcggggggt
     6121 gccgccgtag cgcaccgcga tcgtcatcgc cgcccccgca ggcagcgcct cgtgcagcgc
     6181 gatgtgcagc ttgccggacg acgaccgaaa ctgagctggg cgcttgccgt tcaccgacac
     6241 ctttgtcacc gacagggcgt cggacagatc cagggtgaac gtgcgcaacg ccgcgagcgt
     6301 gacggccgtg atggtggccg atccggacag tcggttgatc gtcaccttgt attcgaggtc
     6361 caactcgtag cgtgacactc gatagccgaa gttgccgttg gcaggcaggt acgggtcaat
     6421 acacggaggg gccgctttct tcgctgcctt cttgctcctc gtcacgcggc gcttgcttct
     6481 gttttcttgg gcggcttcgg cgcatcacca ggcttcttgc ggaacaggtt ccacggcgcg
     6541 atcgggttgc cctgccagcg cgtcgacggc ggtacctcat cgccgcgcat caccagcgag
     6601 gccggtccca ccgtggcgcc cgcaccgatg cgtgcggcgg gcagcgcaac acagtgcgtt
     6661 cccagcgtcg aaccttcttc caggacaacg gtgtccattc gcatgactct gtcgtggaag
     6721 aggtgggttt gcaccacaca accgcggttg accgtcgcgc ccttctcgag ggtgacaagg
     6781 tcggcctcgg gcagccagta ggtctcacac cagacgccgc ggccgatctt ggcgcccagc
     6841 gcgcgtagcc acaggttcat cacgggagtg ccactggcgg cccgggcgaa ccacggtgcg
     6901 gccaccgtct cgacgaacgt gtccgacacc tcgttgcgcc acacgaacga cgaccacaac
     6961 gggtgctcga tggcccgaat acgcccaacc acaagccatt tcgcaactac ggcaatggtg
     7021 ccggccagtg cgcccgcgat cagcaacgcc atgccgctgg ccagggcggc ccacgcccag
     7081 ccgacttcgg tcgccagcca ttgcagcgcg aacaacacac ccacgccgat cgcgaatgtc
     7141 accatcatcg ggatgagtcg gcacgtctcc accaacgcgc gcaggatctt cagccgcagc
     7201 gacgggtgga aggtacgcaa cgcgtcggcc gcggtgggct tgcggcgcaa ccgaaccggc
     7261 ggactgccca accaggacga gccggccttg gccttgtgcg gcgtcgcgga cagcaccgcg
     7321 accaagccgt cgtccgggac tcgtcgaccc ggctgggtga tacccgagtt gccgaggaac
     7381 gcccgcttgc cgatcgttgc cttggcgacg tggatccagc cgccgcccag ctcataggag
     7441 gcgaccatgg tgtcgtcggc caggaacgcg ccgtcctcga cgacggtgaa cttgggcacc
     7501 agcaacgccg tcgagatctc ggttccgcgg ccaactttcg cgccaagcac gcgcagccac
     7561 cacggcgtca acaggcttgc gtaaatcggg aacaggtagt tgcgggcggc gtccatcagc
     7621 cgctccgtgg cccacaactg ccagccgacg cggctgcgca ccgggtgata accctcaggc
     7681 atccacatcg acaacactct gaccccgatc accgtcagcg cggcgtacgt gaccaccgcc
     7741 gccagcgtcg ctgccggagt ccacagcgcg gcgggttcga tcgcgtcgcg cagcgagccg
     7801 gcatcgcgca cgccccaacc gatcacggcc aaacccacgc ccaatgccag caatggcacc
     7861 ccgcccaaca cgatcgacgt cacgccgtac atcgccaccc acagtggtgc ccgccgggga
     7921 cggtggtcag gccacgggtg ccgggcttta ccggatttca ctgcgggcga gcccttccag
     7981 tactgcccgt tcttgacctt gctgaccacg ccggagccgg gcgcgacgtc ggcgttcttg
     8041 cccaccacag caccgggcaa cagggtggtg cgtgcgccga tggtggcgtc gttgccgacg
     8101 gtgatgggac cgacgtggaa gtgatcgccg tcgatccagt gccccgaaag atcgacctcg
     8161 ggttcgatcg agcagcggtg ccccagtcgc agcatcccgg tggcgggcgg agcggagtgc
     8221 aggtcgacgc ccttgccgac cttgttgccc agcatgcggg cgtagtagac cagccacggc
     8281 gcaccggcct gattctcggc gccgctggca tcggcgagcc gttcggccag ccacaccctc
     8341 aggtgcaccg gaccgccgcg gcgatacgtt ccgggtttga ggccgctcag caggatccgc
     8401 gcgcataccg ccgcgatcga catgcgccca agcggcgtaa tgaacagcac gaagccggcg
     8461 gcgatccacc accagttcac cggcaccagc cacggcaccg gatgtagttg cgcgacaaca
     8521 ttgttgagca aagcgagcca caccacccac tgcagcccgg tcagtgtggc gagcggtagc
     8581 gacagcacca cctgcgcggc ctgggtcaac cgcggcgtcg gcttcacgac gcgggtttcg
     8641 accgtgggcg gcgggtcgag ctcgtcgaga tagccggcca gcgacccgag ccgggggtgg
     8701 tcgtagaggt cggccacggt cacctgcgga tagcgttgac gcatcgccgc caccaattgc
     8761 gccgcggaca gcgacccacc gccgagcgca tggaagtcgg cctccggccc gtcgacaggc
     8821 gcggcgagga tgtcgcgcca cagcccggcc agccatccca tcgttccgcc gagatcgggc
     8881 tcctcttcgg cgctttcgcc gggcggcggc cacggcagcg cgtcgcggtc gaccttgccc
     8941 gatgtgcggg tcggcagttc gtcgacgagc accagcctcg gcaccagcgc ggcgggcagc
     9001 gtctcggcca gcgccacgcg ggccttcgcg aggtcgaagg acgggtccgc gctggcgaca
     9061 tagccgacca gcaggggagt gccgctggcg gtccgtcgca ccgccgccgc gccgccgctg
     9121 acg
//
LOCUS       DASRJA010000256         5434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_339284,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000256 DASRJA010000000
VERSION     DASRJA010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339284"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VFC01_11480"
     CDS             <1..1093
                     /locus_tag="VFC01_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HZN80293.1"
                     /translation="LADETKVIAPFFLLTPEWARIPMVLLATAATVIASQAVITGAFS
                     VASQAARLGYLPRLRIEHTSASTIGQIYVPWINGVLMVMVLILVFAFRSSAALAYAFG
                     MAVTGTITIVTTLFFYYARTQWRWPLWGVLIGGGFLLLVDLMFLAANMTKLVHGAWLP
                     LLIATVAFTVMTTWERGREIVTRAREKAEGPLREFVDSLRDSKPPLVRMDGTAVFLNR
                     GKETAPLAMRANVEHNRVLHRHVVVMSIDTVPVPRLSDDERVTIDELGYKRDGIIHAS
                     VCYGYMERPNIPAALHLLDPAHTEGHIDADTASYFLSKLELIAGDEPTMAPWRKRLFI
                     ATSYITADAAAYFKLPLDRTVVIGSRIEV"
     gene            complement(1090..1410)
                     /locus_tag="VFC01_11485"
     CDS             complement(1090..1410)
                     /locus_tag="VFC01_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80294.1"
                     /translation="MWVSVDMAFKLTYTDGQESDYDDDTKWEVDGGVLKLGRGDSDWS
                     VLVSPGHWATIELGKSGKKKDDKAKDKGKDEDKDEDEKAAKNEKADKDEKDDKDDKDD
                     GDDS"
     gene            complement(1421..2137)
                     /locus_tag="VFC01_11490"
     CDS             complement(1421..2137)
                     /locus_tag="VFC01_11490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN80295.1"
                     /translation="MNQPKTQLSGSTAIVTGASRGFGRAIAAALSAAGAEVVGVARTG
                     AGVTADAADPATAIRLIDEYRPRTVVLCAGAAPQMSPLQEQTWESFSQNWNVDVAQAF
                     HWIRYALLRPLAPGSTVIAVSSGAAIKGSPLSGGYAGAKATVRFITGYASLESERAGL
                     GIDFVSVLPALTPATELGTKAVAAYAERQGVDVDTFVQSLQPALTAEQVGKAVLEIAT
                     GEHRDHRAYTLSSAGLAPVG"
     gene            2244..3191
                     /locus_tag="VFC01_11495"
     CDS             2244..3191
                     /locus_tag="VFC01_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN80296.1"
                     /translation="MPAQQEFIEQAAPFRAELIAHCYRMLGSVHDAEDLVQETYLRGW
                     RGYEAFEGRAALRTWLYRIATTACLRALQNRARRVLPAGLGDGSVDPDVDIDASKAGY
                     HWLQPIPDTFTPELAVTAKHSIRLAVVTALQELPARQRAVLILRDVVQFSAAEVAELL
                     ETTPAAVNSALQRARAHLAEVAPTDDDVTEPEDRELLALVERYCAAFENGDMAALTAL
                     LQADVKLEMPPYAVWFTGRDAVTRFLSARALSERGAVLMIPTSANGQPAVADYRRDAA
                     GVMQAHAIHVLTPGRAGIAAITVFLEPDLFEVFGMPTSR"
     gene            complement(3199..3669)
                     /locus_tag="VFC01_11500"
     CDS             complement(3199..3669)
                     /locus_tag="VFC01_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZN80297.1"
                     /translation="MSVEPEASPQPVERFHRLCEIVVARLPFGLNSLVAPTFLGFVVI
                     NSFTFAVDLVLLTLLHGGLGLALPVAATVAYACAFTLSYVLNRVLNFRSHAAVGPQFA
                     VYVVVVVVNYLAFILGVSSGLAAIGVEYHVARIVAGACEAVYMYSAMRWVVFRR"
     gene            complement(3721..4581)
                     /locus_tag="VFC01_11505"
     CDS             complement(3721..4581)
                     /locus_tag="VFC01_11505"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZN80298.1"
                     /translation="MTFPTEPQAAPTCYRHPDRATYVRCTRCNRPICPECMRDAAVGH
                     QCAECVQEGANTVRQPKTSFGGREYGASPVITYALIAINVVMFVLETVSRGLKSELVL
                     FAPAVADGDLYRLVTSAFMHYGVTHLLFNMYALWVIGPPLEKSLGRLRFTALYALSAL
                     GGSVLVYLLSPLNAATAGASGAVFGLFGAAFVVGKRLNLDTRWVVGLIALNLAFTFVI
                     PLLGGANISWQGHIGGLVTGAVVTAAYAYAPRERRTLIQISATVALLVLFTLLIWWRT
                     SDLLAQFGIT"
     gene            complement(4578..4985)
                     /locus_tag="VFC01_11510"
     CDS             complement(4578..4985)
                     /locus_tag="VFC01_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN80299.1"
                     /translation="MRFDLLTTGIEVGLVTTNLDPMVAFYEGFLGLEFQGEVEFPGGS
                     QRRYALGSGVVKLVTYDPPPPAPAAPGGGRAQAGVRYFTIGVSNLRAVAKAFDEAGYE
                     VVEPVTEFAPVPGMGWMFVADPDGNWIELFGTL"
     gene            complement(5007..>5434)
                     /locus_tag="VFC01_11515"
     CDS             complement(5007..>5434)
                     /locus_tag="VFC01_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014984083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN80300.1"
                     /translation="QPHRIRQRLGRSAIGNPAIMIGQLRSVVVDCKDPKGLATFYEGL
                     LGGTIDAEEDTWVVLTEPGGRRLAFQYSPRHQPPKFPDPAGSQQFHLDILVNDVDDAE
                     REVRQLGATRVDGAVGEDQFRVFRDPAGHTFCLVWGVDP"
BASE COUNT          944 a         1842 c         1726 g          922 t
ORIGIN      
        1 gctcgcggac gagaccaagg tgatcgcgcc gtttttcctg ctcaccccgg agtgggcacg
       61 aattccgatg gtgctgcttg cgacagcggc gacggtcatc gcgtcgcagg cggtgatcac
      121 cggggcgttt tcggtggcgt cccaggcggc ccggctcggc tacctgccgc ggctgcgcat
      181 cgaacacacc tcggcgtcga cgatcggcca gatctacgtg ccgtggatca acggcgtgct
      241 gatggtgatg gtgctgatcc tcgtcttcgc cttccgcagc tctgccgcgc tggcctacgc
      301 gttcggcatg gcggtgacgg gcacgatcac catcgtcacc accttgttct tttattacgc
      361 ccgtacccag tggcgatggc cgctgtgggg cgtgctgatc ggcgggggct tcctgctgct
      421 ggtggatctg atgttcctcg cggcgaacat gacgaagctg gtgcacggcg cctggctgcc
      481 actgctcatc gcgaccgtcg cgttcacggt gatgacgacc tgggagaggg gtcgtgaaat
      541 cgtcactcgc gcaagggaaa aggccgaagg cccgctgcgc gagttcgtcg acagcctgcg
      601 cgactccaaa ccgccgctgg tgcgcatgga cggcaccgca gtgttcctca accgcggcaa
      661 ggagaccgcc ccgctggcga tgcgggccaa cgtcgagcac aaccgggtgc tgcaccgcca
      721 cgtcgtcgtc atgtcgatcg acacggtgcc cgtcccccgg ctgtccgatg acgagcgggt
      781 caccatcgac gaactcggct acaaacgtga cggcatcatc cacgccagcg tgtgctacgg
      841 gtacatggaa cgaccgaaca ttccggcagc gctacacctg ctcgaccccg cccataccga
      901 aggccacatc gacgccgata cggcttccta cttcctgtcc aaactggagt tgatcgcggg
      961 cgacgaaccg acgatggcac cgtggcgcaa gcgactgttc atcgcgacct catacatcac
     1021 cgccgacgcc gccgcgtact tcaagttgcc gctggaccgg accgtggtca tcgggtcgcg
     1081 catagaggtc taagagtcgt cgccgtcgtc tttatcgtct ttatcgtctt tttcgtcctt
     1141 gtcggctttc tcgttcttgg ccgctttctc gtcctcgtct ttgtcctcgt ccttgccttt
     1201 gtctttggct ttgtcgtcct tctttttccc gctcttgccg agttcgatcg tggcccagtg
     1261 ccccggcgag acgagcacag accagtcgct gtccccgcgg ccgagcttga gcacgccgcc
     1321 gtcgacctcc cacttggtgt cgtcgtcgta gtcggattct tgtccgtctg tgtatgtgag
     1381 tttgaacgcc atgtcgacgg atacccacat ttgcgcctgc tcagcccacc ggagcgaggc
     1441 cagccgagga cagggtgtac gccctgtgat cgcggtgctc acccgtggca atctccaaca
     1501 ccgcctttcc gacctgctcg gcggtgagcg cgggctgcag ggactggacg aacgtgtcga
     1561 cgtcgactcc ttgccgctcg gcgtacgcag cgacggcctt cgtgccgagc tcggtcgccg
     1621 gcgtcaacgc gggtaagacc gagacgaagt cgatgcccag cccggcgcgt tccgattcaa
     1681 gtgaggcgta gccagtgatg aacctgacgg tggccttggc gcccgcgtac ccgccactga
     1741 gcggcgagcc cttgatcgct gcaccgctcg ataccgcgat gacggtgctg cccggcgcca
     1801 acgggcgcag cagcgcgtac cggatccagt ggaacgcctg cgccacatcc acattccagt
     1861 tctgactgaa cgactcccac gtctgctcct gcaacggact catctgcggc gccgctcccg
     1921 cacagagcac cactgtgcgc gggcggtatt cgtcgataag ccggatggca gtagcagggt
     1981 cagcagcgtc ggcggttacc ccggcaccgg tacgggcgac tccgacgacc tcggccccgg
     2041 ccgccgagag agcggcggcg atggcgcggc cgaatccgcg gctggcaccg gtgacgatcg
     2101 cggtggagcc ggacaattgg gttttgggtt gattcatatt gagtcagata cacagcaccg
     2161 acgcgattca tcggttctgt gcgatgaatc ccggccaccc ggcgtatctc tatttgaggg
     2221 gtgcacccgt taggagaacc gccgtgccgg cccagcagga attcatcgaa caggcggcgc
     2281 catttcgcgc cgagttgatc gcgcactgct atcgcatgct cggctcggta cacgacgccg
     2341 aggacctggt ccaggagacg tacctacgcg gctggcgcgg ctatgaggcg ttcgagggcc
     2401 gggcggccct gcggacctgg ctctaccgga ttgcgacgac cgcgtgtctg cgcgcattgc
     2461 agaaccgtgc ccgccgcgtg ctgcccgccg gactcggtga cggttccgtc gaccccgacg
     2521 tcgacatcga cgccagtaag gcgggctatc actggctgca gccgattccc gacaccttca
     2581 cacccgagct cgctgtcact gccaaacaca gcatccggtt ggccgtcgtt acggcgctgc
     2641 aggaactacc ggcccggcag cgggcggtgt tgatcctgcg cgatgtcgtg cagttcagcg
     2701 ccgccgaagt ggccgagctg ctcgagacca caccggcagc ggtcaacagt gcgctgcagc
     2761 gggcgcgggc acatctcgcc gaggtcgccc cgaccgacga tgacgtcacc gagccggaag
     2821 accgcgagct gctggctctc gtcgaacgtt attgcgccgc attcgaaaac ggtgacatgg
     2881 ccgcgctgac cgcactgctg caggccgacg tcaagctcga aatgccgccg tacgcggtgt
     2941 ggttcaccgg tcgcgacgcg gtgacgcgat tcctctcggc acgcgctctc agcgagcgcg
     3001 gcgccgtgct gatgatcccc acgtccgcca acgggcagcc cgcggtggcg gactaccgcc
     3061 gcgacgccgc cggcgtgatg caagcccatg cgatacacgt actgacccct ggccgcgctg
     3121 gaatcgccgc gatcacagtg tttctcgagc cggacctgtt tgaagtgttc ggtatgccaa
     3181 caagtcggtg agcgccggtc agcgccggaa caccacccac cgcatcgcgc tgtacatgta
     3241 gaccgcttcg caggcgcccg ccacgatgcg ggcgacgtgg tattcgacac cgatggccgc
     3301 cagcccgctg gagacgccga ggatgaacgc gaggtagttc accacgacca ccaccacgta
     3361 caccgcgaac tgcgggccca ccgcggcatg ggagcggaag ttcaagacgc ggttgaggac
     3421 gtagctcagc gtgaacgcgc atgcgtaggc caccgttgcc gcgacgggca gcgccaggcc
     3481 cagcccgccg tgcagcagcg tgagcagcac cagatcgacg gcgaacgtga agctgttgat
     3541 caccacgaag cccagaaatg tcggcgccac aagggaattc agcccgaatg gcaggcgcgc
     3601 gacgacaatt tcgcacagcc gatggaaacg ttcgaccggc tgcggggacg cctcgggctc
     3661 cacgctcata acgccacgct gacacgggca gatggctaat cgctgagcac cgggcaaacg
     3721 tcaggtgatg ccgaactggg ccagcagatc gcttgtccgc caccaaatca gcagcgtgaa
     3781 caagaccagc agggcaaccg tcgccgaaat ctggatcagc gtgcgccgct cacgtggtgc
     3841 atacgcgtag gccgcggtga cgactgctcc ggtgaccaac ccgccgatgt ggccctgcca
     3901 gctgatgttg gccccgccga gaagcggaat gacaaacgtg aaggccaagt tgagcgcgat
     3961 gaggccgacc acccaccggg tgtcgaggtt gagccgcttg cccaccacga acgccgcccc
     4021 gaacagtccg aacaccgcac ccgacgcacc cgcggtcgcc gcgttcagcg gtgaaagcag
     4081 gtagaccagg acagaaccac ccagcgcgct caacgcatac agcgcggtga agcgcagcct
     4141 gcccaacgac ttctccagcg gcggcccgat gacccatagc gcatacatgt tgaacagcag
     4201 gtgtgtcacg ccgtagtgca tgaacgccga cgtcaccagg cggtacagat cgccgtcggc
     4261 gacggccggc gcgaacagca cgagctcgct cttcagcccc cgcgacaccg tctcgagcac
     4321 gaacatcacc acgttgattg cgatcagcgc gtaggtgatc acggggctcg ccccgtactc
     4381 tcgcccgccg aagctggtct tcggctggcg caccgtattc gcgccttcct gcacgcactc
     4441 cgcgcactgg tgtccgacgg cggcgtcccg catgcactcg gggcagatcg gacggttgca
     4501 ccgggtgcag cggacgtacg tcgctcgatc gggatggcgg taacacgtgg gcgccgcctg
     4561 cggctcggtc gggaacgtca tagcgtgccg aacaattcga tccagttgcc gtcggggtcg
     4621 gcgacgaaca tccagcccat gccgggcacc ggtgcgaact cggtcaccgg ctcgaccact
     4681 tcgtacccgg cctcgtcgaa cgccttggcc actgcgcgca ggttgctcac cccgatcgtg
     4741 aagtaccgca cgcccgcttg cgcgcggccg cctccggggg cggcaggtgc gggcggcggc
     4801 gggtcgtacg tcactagctt caccacgccg ctgccgagcg cgtaccggcg ctgcgacccg
     4861 ccggggaact cgacctcacc ctggaactcc agcccgagga acccctcgta gaaggcgacc
     4921 atcgggtcga gattggtggt gacgagaccg acttcgatgc cggtggtcag caggtcgaat
     4981 cgcacgccac gagactacgc accgccctac gggtcgaccc cccacaccag gcagaaggtg
     5041 tggcccgccg gatcgcggaa gacccggaac tgatcttcgc cgacggcacc gtccacgcgg
     5101 gtggcgccca gctgacgaac ttctcgctcg gcgtcatcga cgtcgttgac gaggatgtcg
     5161 aggtgaaact gttgagaccc agcggggtcg gggaactttg gtggttggtg tcgtggcgag
     5221 tactggaacg ccagcctgcg gccaccaggt tctgtcagca ccacccaggt gtcttcctcg
     5281 gcgtcgatcg tgccgccgag aaggccttcg tagaacgtcg ccagaccctt cgggtccttg
     5341 cagtcgacca ccaccgagcg aagttgtccg atcatgatcg ctgggttacc aatcgcgctg
     5401 cgccctaacc gttgccgaat ccggtgcggc tgcg
//
LOCUS       DASRJA010000257         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4090876,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000257 DASRJA010000000
VERSION     DASRJA010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4090876"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFC01_11520"
     CDS             complement(<1..281)
                     /locus_tag="VFC01_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZN80301.1"
                     /translation="MIAHPAGPRHAEEVHHGGAPPRPQSPAEMLNLAPNVWPRNTVRG
                     ADGVVSIAGVRITDIAAEFGTPVFVIDEDDFRSRCREIAQAFGGGEYVH"
     gene            complement(278..1930)
                     /gene="argS"
                     /locus_tag="VFC01_11525"
     CDS             complement(278..1930)
                     /gene="argS"
                     /locus_tag="VFC01_11525"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZN80302.1"
                     /translation="MTPADLAELLKTTAAAVLSEHGLDTSALPATVTVERPRNPEHGD
                     YATNIALQLAKKVGANPRELAGWLATALANQDGIASADVAGPGFVNLRIEASAQGVIV
                     NNVITSGANYGDSDALKGSNINLEFVSANPTGPIHIGGTRWAAVGDALGRLLATQGAK
                     VVREYYFNDHGAQIDRFSNSLIAAAKGEPTPDDGYAGDYIQEIAAQVLVKEPDALSLP
                     DAEQRETFRAIGVDLMFTHIKQSLHDFGTDFDVFTHEDSMHTSGRVDQAIAKLREAGK
                     VYEKDGAVWLRTTNFGDDKDRVVIKSDGAPAYVAADIAYYLDKRERGFDLCIYMLGAD
                     HHGYIARLKAIASSLGEDPATVEVLIGQMVNLVRDGQPIRMSKRAGTVITLDDLVESI
                     GVDAARYSLIRSSVDTPIDIDLELWSSASNENPVYYVQYAHARLSALARNAAELGLSP
                     SAEHLELLTHDKEGTLIRNLGEFPRVLKTAAALREPHRVSRYLEDLAGDYHRFYDSCR
                     VLPQGDESPSDLHAARLALCQATRQVIANGLAILGVTAPERM"
     gene            2035..2107
                     /locus_tag="VFC01_11530"
     tRNA            2035..2107
                     /locus_tag="VFC01_11530"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2068..2070,aa:Arg,seq:ccg)
BASE COUNT          352 a          662 c          815 g          391 t
ORIGIN      
        1 tagtggacgt actcgccgcc gccgaaagct tgggcgattt cacggcagcg cgaccggaag
       61 tcgtcctcgt ctatcacgaa gaccggggtg ccgaactcag ccgcgatgtc agtgatgcgc
      121 acgccggcga tcgacaccac accgtcggca ccgcgaacgg tgttgcgcgg ccacacgttc
      181 ggggcaaggt tgagcatttc ggccggggac tgcggccggg gcggggcgcc gccgtggtgg
      241 acttcctcag cgtgccgggg cccggcggga tgagcgatca catgcgctcc ggcgcggtaa
      301 caccaaggat ggcaaggccg ttcgcgatga cctggcgggt ggcctggcac agcgcgagtc
      361 gcgcggcgtg cagatcgctg ggcgattcgt cgccctgcgg cagcacccgg caggagtcgt
      421 agaaccggtg atagtcgccg gccaggtcct ccaggtagcg ggagacgcgg tgcggttcac
      481 gcagggccgc agcggttttc agaacacgcg ggaattcgcc gaggttgcga atcagcgtgc
      541 cttccttgtc gtgcgtgagc agctccaggt gctcggcgct gggactgagg cctagctcgg
      601 cagcattgcg ggccagggcc gacagccggg cgtgcgcgta ttgcacgtag tagaccgggt
      661 tttcgttgga cgccgacgac cacaactcga gatcgatgtc gatcggagtg tcgaccgaag
      721 agcggatcag cgaataccgg gcggcgtcga cgccgatcga ctcgacgagg tcgtcgagcg
      781 tgatgacggt gccggcgcgc ttgctcatcc ggatcggctg gccgtcgcgg accaggttga
      841 ccatctgccc gatgagcacc tcgacggttg cggggtcctc gccgagggat gaggcgatgg
      901 ccttgagccg ggcgatgtag ccgtggtggt cggcgccgag catgtagatg cacaggtcga
      961 acccgcgctc gcgcttgtcg aggtagtagg cgatgtcggc ggcgacgtag gcgggcgcgc
     1021 cgtcgctctt gatgacgaca cggtccttgt cgtcaccaaa gttggtggtg cgcagccaaa
     1081 ctgcgccgtc cttctcgtag accttgccgg cttcgcgaag cttggcgatg gcctggtcga
     1141 cccgaccgga ggtgtgcatc gagtcttcgt gggtgaagac gtcgaagtcg gtgccgaagt
     1201 cgtgcagcga ctgcttgatg tgggtgaaca tcaggtcgac gccgatggcg cggaaggtct
     1261 cgcgttgctc ggcgtcgggc aggctcagcg cgtcgggctc cttgacgagc acctgggcgg
     1321 cgatctcttg gatgtagtcg ccggcgtagc cgtcgtcggg cgtcggctcg cccttggctg
     1381 cggcgatcag ggagttggaa aaccggtcga tctgggcacc gtggtcgttg aagtagtact
     1441 cacgcacaac cttggcgccc tgggtggcga gcagccggcc cagcgcgtcg ccgacggcgg
     1501 cccagcgggt gccaccgatg tggatgggcc cagtcgggtt ggcggagacg aactcgaggt
     1561 tgatgttgga gcccttgagc gcgtcggaat cgccgtagtt agcgcctgag gtgatgacgt
     1621 tgttgacgat gacgccctgg gcggaagcct cgatgcgcag gttgacgaac ccgggccctg
     1681 cgacgtcggc cgaggcgatg ccgtcttggt tggccaaggc ggtcgccagc cagccggcca
     1741 gctcacgggg gttggcgccg accttcttgg cgagctgaag ggcaatgttg gtggcgtaat
     1801 caccatgctc gggattgcgg ggacgctcga cggtgacggt cgccggcaac gcggaggtgt
     1861 caaggccatg ctcggacagc accgcggcgg cggtggtctt gagcagctcg gccaggtcgg
     1921 cgggggtcac gagggtccat cctatggtct tgggtgatca gcccccgaat ccgtttcccg
     1981 tcgtctgaat gcgttaggct gtcggagccc atcggcgcac tctcttcgat gtgcgccccc
     2041 gtagctcagg ggatagagcg tctgcctccg gagcagaagg ccgcaggttc gaatcctgcc
     2101 gggggcacaa gccttagatg caggtcacag gcgatttccg cctcagccta acaggccccg
     2161 tgccccaagc atgcctttcc gttgccaacg tgccgaggcc cggctgacaa gaaaagcccc
//
LOCUS       DASRJA010000258         4855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2593861,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000258 DASRJA010000000
VERSION     DASRJA010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2593861"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VFC01_11535"
     CDS             <1..530
                     /locus_tag="VFC01_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZN80303.1"
                     /translation="ARATLVQYRRSTAPPGRLWTAPISLVQPPPPDDSILPFVGSDDA
                     GWTRSPAAHQNDSRKRFYNDGIKVLAGEKNSPFVRAVMIAEATSLVTNLGTQGIGYIN
                     GDLTAAFSRLPVDDWICIQGDSHWASDGISVGTATLFDTRGPFGSGMVTAVANPAAQI
                     DFSSESIPLKGLNYE"
     gene            complement(543..1337)
                     /locus_tag="VFC01_11540"
     CDS             complement(543..1337)
                     /locus_tag="VFC01_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80304.1"
                     /translation="MTEREAPPDWGFDYRQGDPYDDDGWFGREQVADPMSVGEVAAHP
                     RMEQVSYVREERYEAPREVEPPREVEPPREVDRPAPPTRRLVSPPTPWTVNGQLRAPN
                     NSTLTFRSPPPPWYRTKQTKVALIAAAAAAVVVPIVVLLWPDSSSTSPGQSTSVSPQA
                     PTSTQPAPRSVRPTPTNAPPPPPPPPQPPPPPQPPPPPAEDTGSAPTYNQPQWTRPAA
                     PEPTQKPEVDVTRAPISVAPTPRKPSESNSSTPGDAPERRGCFGWC"
     gene            complement(1430..2206)
                     /locus_tag="VFC01_11545"
     CDS             complement(1430..2206)
                     /locus_tag="VFC01_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80305.1"
                     /translation="MTQPLIKYGDPLRIVDQLDETGVVCLENAIPTEWLADVRAEVQD
                     WLLNHGVRDHLIDLPQGEGQSAADAFVNNRAVVSLLSEIARTRFPDAEPELIVSALRV
                     AAEPRGESDAFGFHYDASIVTMIVPIFLPDAEPGNAGEFVALLNKRPFRRFVLVHIID
                     KAIGQSRFYRSHILRRLDRTGDLKKIDMEVGNLYLFWGYRSLHGNMPCESGALRTTLL
                     LHFGRTHVSSKTLTAAARLGRSLRTLGQKSTATEATVGAY"
     gene            complement(2655..2999)
                     /locus_tag="VFC01_11550"
     CDS             complement(2655..2999)
                     /locus_tag="VFC01_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80306.1"
                     /translation="MDGSSVSDAVELACAPPVLWTTRVLGSGVADESVDDEPSVSVVV
                     VVDAELGVRFDGLSLSVDDEADVLDEPDGEELDDELVSDGSARATPGMVATADPIPSA
                     TANAPTRPTYLT"
     gene            complement(3021..3251)
                     /locus_tag="VFC01_11555"
     CDS             complement(3021..3251)
                     /locus_tag="VFC01_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80307.1"
                     /translation="MAGMARVNVRRSDPDVALVNRNALGDCDVDVLAAGSPCGIEEVA
                     AAADVLGDDFLAPSVSAFGFGGDGVGVFETPS"
     gene            3241..>4855
                     /locus_tag="VFC01_11560"
     CDS             3241..>4855
                     /locus_tag="VFC01_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZN80308.1"
                     /translation="MPATESSPTALADTPTAAVAPRVASAPFSGPLAPLNIVTSVLST
                     VLEWVGFGPSMTASPETPVQTPLLWTLLAWVRREIEQTLNPTFAAHTQPVAALTVESP
                     NLLVNPGAELGDPSLSGYSSVTVPGWTVTGTPTVIEYGTLRRFPWPTASPGPTFPAFL
                     GFPTSACAPADSGEQFFGGGPVATSTLTQTVDLRAAAVDIDSGTVPYELSGALGGFII
                     DPSAASVTVEFLGANGLLLDTGGIRPVTVLDRFFLTRLIERETTGMIPVGTRSARVVV
                     TLKDCNPILGNYNNAYADNLSFTVGATGLAPAPLTPPLSTVGDLDHVFMVYMENKGFS
                     DIVGSPNAPYLNGLIGNYGLASNYYALTHPSDPNYYPILGASDFGINYNCPANCFDQP
                     NLADNIELARKTWAGYMVGGGGYSTPTDRLPFLAFSDIYNDPARVTGHLFDLTEMAGD
                     LVDPDTAPDFVWFAADDATNMEGPTDTLIGIIQWALSQLTPKVLGGHQYNVAAGDAWL
                     KETLPLIMDSNTWNDPGERSAIFLTFDEDY"
BASE COUNT          932 a         1523 c         1495 g          905 t
ORIGIN      
        1 tagcgcgggc cacgctggtg caataccggc gctcgactgc gccgcccggc cgactatgga
       61 ccgcgccaat ctcactcgta cagccgccgc caccggacga ctcgatactg ccgttcgtgg
      121 gtagcgatga tgcgggttgg acgagatcgc cggcggcgca tcagaacgac tcgcgtaagc
      181 gcttctacaa cgacggcatc aaagtgcttg cgggggagaa gaactcccca tttgtccggg
      241 ccgtcatgat cgccgaggcg acaagtttgg tgaccaatct gggtacgcag ggcatcggct
      301 atatcaacgg tgacctgaca gcggcattct ctcggctacc cgttgacgac tggatctgca
      361 tccaggggga ttcgcactgg gcgtcagacg ggatctccgt cggcacagcg acactgttcg
      421 atactcgcgg tccgttcggt tcgggaatgg tcaccgccgt ggccaatccc gcagcccaga
      481 tcgacttttc gagcgaatca attccgttga agggtctcaa ctacgagtaa cggtcatgca
      541 ggtcagcacc aaccaaaaca cccccgcctt tcaggggcgt ccccgggagt ggatgagttg
      601 ctttcggatg gcttacgcgg agttggcgcc acactgatcg gcgcgcgggt gacatcgact
      661 tccggcttct gagttggctc cggtgcggcg ggacgcgtcc attggggctg gttgtacgtc
      721 ggggcggagc cggtgtcctc ggccggaggt ggcggcggct gcgggggtgg cggcggctgc
      781 gggggcggtg gaggcggcgg gggagcattg gtcggagttg ggcgaacgct gcgcggggca
      841 ggctgggtgc tcgtcggtgc ctgtggtgac accgaggtcg actgcccagg gcttgtcgaa
      901 gaactatcgg gccacaggag cacgacaatc ggcacgacca cagccgcggc cgccgcggcg
      961 atcagcgcga cctttgtctg cttcgtgcgg taccacggcg gcggcgggga ccggaacgtc
     1021 aacgtgctgt tgttcggggc gcgaagctga ccgttcaccg tccatggggt aggtggtgaa
     1081 acgagccgac gggtcggcgg tgcagggcgg tcgacttcgc gaggaggctc gacttcgcgc
     1141 gggggttcga cttcgcgcgg ggcctcgtat cgttcttccc ggacgtagct gacttgttcc
     1201 atgcgcggat gtgcggcgac ttcaccgact gacatgggat cggcaacctg ctcgcgaccg
     1261 aaccacccgt cgtcgtcata cgggtcgccc tgccggtaat cgaagcccca atccggcggg
     1321 gcctcgcgct cagtcacgta ccgatgctaa cgcccacgtg cagggcggtg gcgaatcaga
     1381 cgctatcggg gcagttcacg catggaactg cggtatcaac ctatttcgat tagtaagcgc
     1441 cgaccgtcgc ttctgtggca gtggatttct ggcccagcgt gcgtagcgac cgtccgagcc
     1501 tagcggcggc tgtcaatgtt ttgctcgaca cgtgggtgcg gccgaaatgc aggaggaggg
     1561 tggttcgcaa agcgcctgat tcgcatggca tgttgccatg taggctgcga tagccccaga
     1621 acagataaag gtttccgact tccatgtcga tctttttcaa atcgccagtg cggtcgagcc
     1681 tccgcaggat gtgactgcga tagaacctgc tctgcccaat ggctttatca atgatgtgga
     1741 caagaacaaa gcgccgaaaa ggccgcttgt tgagtagggc tacgaattcg cccgcgttac
     1801 cgggttcggc gtcgggcaaa aatatcggca caatcatcgt tacgatactg gcatcgtaat
     1861 gaaacccaaa tgcgtcactt tcgccccgtg gttcggcagc tacgcgcaat gcagaaacga
     1921 tgagctccgg ctcggcgtct ggaaagcggg tgcgcgcaat ctcactaagc agcgacacaa
     1981 ctgcgcgatt attgacgaaa gcatctgcag cgctttgacc ttcaccctga ggtaggtcga
     2041 tgagatggtc gcgcacacca tgattcaaca gccaatcctg aacctcggcg cgcacatcag
     2101 ccagccattc tgtcggaata gcattctcaa ggcacaccac tccggtttca tcaagctggt
     2161 cgacgattct gagtgggtcg ccatatttga ttagcggctg cgtcattgtc ggagaagtat
     2221 aagtgatctc gacgaattga agtatcggtg aatcgatcgt gtgagccacc ccacatttaa
     2281 gctgattgcg ggctgtgcgc atgctatgcg ggtgctgttt gggtccttgg cgggcacaat
     2341 ttaagccgat gtcaaacgat ggccccgtgg ctaaatgtgt gtttcgtgag tcaaagggca
     2401 gggggatacc gaatccgtca cagtgacgta tgacgtggcc gtagacgttt cgggtgagca
     2461 gcgttccggg gcaagcagat tcgatacgat gctcaatcgc catcgtcaaa cgggggcgac
     2521 cgggcttcgc cgggtcccgc gagcggtcgg atccactcag caaaagtctt cgatcgtttc
     2581 gaccctcgcc gatcgtcatc ggcgtaaatt ccttggcgct gcagatccct cggcgagcaa
     2641 ggagccatga tgggttacgt caggtatgtc ggccgggtcg gggcgttagc agtcgcgctc
     2701 ggaattggat ctgcagtagc gaccatgccg ggcgtggccc tagcggaacc atcggacacg
     2761 agctcgtcgt ccagctcctc accgtcgggt tcgtcgagta cgtcggcttc gtcgtccact
     2821 gacaaggaca atccgtcgaa cctcacgccc agttcggcgt cgacgacaac aacgacggat
     2881 acggacggct cgtcgtcgac ggattcgtcg gcgacgcccg atccgagcac ccgcgtcgtc
     2941 caaagtacgg gcggcgcaca ggcaagctca acggcatccg agaccgacga accatccacg
     3001 gccggcagct catccggtga tcacgatggc gtctcgaaca cgccgacacc atcaccacca
     3061 aagccgaaag ccgatacaga cggcgccaag aagtcatcac caagcacgtc agcagcggcc
     3121 gcgacttctt cgataccaca aggggatccg gcggccagca cgtcaacgtc gcagtcgccg
     3181 agcgcatttc gattgaccaa cgcgacatcg ggatcggacc ttcgaacatt caccctggcc
     3241 atgccggcca cagagtcatc gccaacggcg ttggcggata caccaaccgc cgcggttgcc
     3301 ccgcgggtcg cttccgcccc gttcagtgga ccgttggcgc cgctgaacat cgtcacgagc
     3361 gtgctgtcga ccgtgctgga gtgggtcggc ttcggcccat cgatgaccgc cagtccggaa
     3421 acgccggtgc agacgccgct gttgtggacg ctgttggcct gggtgcgccg cgagatcgag
     3481 cagaccctca atccgacgtt cgccgcacac acacaaccgg tcgccgcgct cacggtggag
     3541 agcccgaact tgctggtgaa cccgggtgcc gaactcggtg acccatcctt gtcgggatac
     3601 agttcggtga ccgtgccggg ctggacggtg accggcacgc cgaccgtcat cgaatacggc
     3661 accctgcgcc gttttccgtg gccgaccgca tcgccagggc cgacattccc ggcgtttctc
     3721 ggttttccca cctccgcgtg cgcgcccgcc gacagcggcg agcagttctt cggtggtggg
     3781 cccgtcgcga catccacact tacccaaact gtcgacctca gggccgccgc agtcgacatc
     3841 gactccggca cagtgcctta cgagctgagc ggagccctcg gtggcttcat catcgatccg
     3901 tcagccgcct cggtgaccgt cgagttcctc ggcgccaacg gactcctgct ggacaccggc
     3961 ggaatccggc cggtcaccgt gctggaccgc ttcttcctga ctcggctgat cgaacgcgaa
     4021 accacgggga tgattccggt gggcacccgc agcgcgcggg tggtcgtgac gctaaaggac
     4081 tgcaacccca tactcggcaa ctacaacaac gcctatgccg acaacctctc gttcacggtc
     4141 ggcgctacgg gcctcgcccc ggcaccactc acgccaccgt tgtcgactgt cggcgacctc
     4201 gatcacgtgt tcatggtcta catggaaaac aagggctttt ccgatatcgt cggcagccca
     4261 aacgcgccgt acctcaacgg tcttatcggc aactacggcc tcgcgtcgaa ttactacgcg
     4321 ctgacccacc ccagcgaccc gaattactat ccgatcctcg gtgcatcaga cttcggcatc
     4381 aattacaact gccccgcgaa ctgcttcgac cagccgaacc tggccgacaa catcgagttg
     4441 gcgcgcaaga catgggcagg ctatatggtg ggcggaggcg gttacagcac gcccaccgac
     4501 cggctgcctt tcctcgcctt cagcgacatc tacaacgacc ccgcccgcgt cacgggccac
     4561 ctgttcgacc tgaccgaaat ggcgggtgat ctcgtcgatc ctgacaccgc cccggacttc
     4621 gtgtggttcg ctgccgacga cgccaccaac atggaaggac ccaccgatac cctcatcggc
     4681 atcatccaat gggctctgag ccagctcact ccgaaagtgc ttggcggaca tcagtacaac
     4741 gtggccgcgg gcgacgcctg gctcaaggag acgctgcccc tcatcatgga ctcgaacacc
     4801 tggaacgacc ccggtgagag aagcgccatc ttcctgacgt tcgacgagga ctaca
//
LOCUS       DASRJA010000259         5198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_327434,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000259 DASRJA010000000
VERSION     DASRJA010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327434"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VFC01_11565"
     CDS             complement(<1..886)
                     /locus_tag="VFC01_11565"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN80309.1"
                     /translation="MPQAFDGIRVLELGQIYNGPYCGLLLAQLGADVIKIEPPGGEQL
                     RFRSHQPVESHEFVMLNSNKRSVVLNLKTDAGRQALLDLVETADVLIENFAPGTMERL
                     QLGAARLLEHNPRLVVACGKGYGSTGPYAHMSAMDITVQAMSGSASSTGQSDGPPTKS
                     GAAFVDFSGGIHLFGAISAALFQRERTGRGQIVEVSMHDTIYPMLASSLGGLHNNPDR
                     ELPERTGNRHSGMAVAPYNIYEASDGWLAIICISERHWRGVATALGRPELIEDPRFRT
                     ERDRVVNIDAVDAEVSSVT"
     gene            complement(898..1452)
                     /locus_tag="VFC01_11570"
     CDS             complement(898..1452)
                     /locus_tag="VFC01_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011594009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HZN80310.1"
                     /translation="MTAPVSPGMFRTDSGVPLPLVALPQDDLLTVNIDQIPLLKDAMG
                     PGIHIQPLRLDPEKGQVVLMARMAPGCLLPIHYHTGTAEVYTFSGCWKYAEYPTQPQT
                     AGSYLYEPGGSVHTFYCPEDNTEDTVVLLWMEGAQIGFNQDGTLAHINDATSIQHVTE
                     TVSAAQGTGPVGYIHGGAAGVVGS"
     gene            complement(1806..2354)
                     /locus_tag="VFC01_11575"
     CDS             complement(1806..2354)
                     /locus_tag="VFC01_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009679971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HZN80311.1"
                     /translation="MTTRDESPSTATDDLPQVNPPASVSRRVRAYAAFLRTGVGRWLA
                     KSIAPKVDPWLLRATGGRLAMGLMLPSALLTTTGAKTGQPRTNPVFYFHDGPDVIVIA
                     SNYGADRHPAWYHNLSANPRAQIATNGGGPVLTASAVSDAAERKRIWAMADRVYPLWP
                     DYRRRAARCHRTIPIIRLRADA"
     gene            complement(2351..2914)
                     /locus_tag="VFC01_11580"
     CDS             complement(2351..2914)
                     /locus_tag="VFC01_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011593997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HZN80312.1"
                     /translation="MTAADEDQPRVGPTTGGPYGLYLRVMRRIGHYGWFSLFMKHVGS
                     RVDRVLIRASRGRFSMSGPRLPTMLLTTRGRRTGKDRTVPLHYVRDGKNLVAACENFG
                     LEAASSWPKNLLADPKARIEIGGTAATYLSRPATDDEVARNMPRLIEMWPAHDSHMER
                     NGVRQVIIFEPSGHGRDHQVAEGRPSP"
     gene            3044..3784
                     /locus_tag="VFC01_11585"
     CDS             3044..3784
                     /locus_tag="VFC01_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009473383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZN80313.1"
                     /translation="MPARPHRTVDRVVEILETVSLSQRGVTLAELATAMGAAKSSVQE
                     LTNGLLARGYLIEEDRRFHLGPGPFILAARANKLAALSLNHEFVAELSKVLDCTVLVG
                     VRVGDSIVFTDFVGEESPSLSFVARTHARRPLYTSAAGKTLLANVPDDEMYRLLDLVS
                     PEQAGEVRQFLAELPEIRSRQLAFNRGITLADAYAVATPLLAPDGSLIAVISAAVDAS
                     QANRLDDIGEELKNSVASLGPKYGLDTR"
     gene            complement(3836..4873)
                     /locus_tag="VFC01_11590"
     CDS             complement(3836..4873)
                     /locus_tag="VFC01_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN80314.1"
                     /translation="MLRIDTHHHMIPPDYRDILRQAGIDEAGGRALPNWSPEGSLQTM
                     DELGVATAILSVSTPGTTFLSNPADAVALARDLNDYSAHLVASQPERFGFFATVPMPH
                     INESVVEAVRALDDLNADGVVLLANNDGTYLGEDGQDDLWAALDARSAVVFIHPADLP
                     GPSVPGVAPFAADFLLDTTRAAYLLVRNGIRRKYPNIKLILSHAGGFVPYASHRMAVA
                     IMAETGASPADTLDDFSSFYFDTALSSSAAALPTLLAFAKPGHITFGSDWPFAPTAAG
                     QYFAVGLETYPNLDAASRAAIDRTNALALFPRLGTAPPLIPVGGRDRAKAALRRQALR
                     LTVKAVNPKTG"
     gene            complement(4860..>5198)
                     /locus_tag="VFC01_11595"
     CDS             complement(4860..>5198)
                     /locus_tag="VFC01_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80315.1"
                     /translation="GRSFRAFVHTRLGPALHAAGACDVRTYGFLALAPVAHSTFGVAH
                     DNPPHRRYHGAVIFGTRGRDEVEDVIASSEVAAAVSDQHLFCTAVHAFGVGRTVPVIR
                     TTARDIDVAN"
BASE COUNT          867 a         1650 c         1725 g          956 t
ORIGIN      
        1 tggtgaccga cgacacttcg gcgtcgaccg cgtcgatgtt gacgacccgg tccctctcgg
       61 tgcggaacct cgggtcttcg atcagctcgg gccggcccag cgcggtcgcc acgccacgcc
      121 agtgccgctc ggagatgcag atgatcgcca accacccgtc gctcgcctcg tagatgttgt
      181 acggcgcaac cgccatcccc gagtggcggt taccggtgcg ctcgggcaac tctcggtcgg
      241 gattgttgtg cagcccaccg agactcgacg ccaacatcgg atagatggtg tcgtgcatcg
      301 acacctcgac gatctggccc cgccccgtgc gctcgcgctg gaacagggcc gcgctgatcg
      361 ccccgaatag atgaatgccg ccggagaagt ccacgaacgc ggccccggac ttggtcggcg
      421 gcccgtcgga ctgtcctgtg ctcgaggcgc tgcccgacat cgcctgcacg gtgatgtcca
      481 tcgccgacat gtgggcgtac ggacccgtag acccgtagcc cttgccgcag gccacgacga
      541 gacgcggatt gtgctcgaga agtcgcgccg cgcccagctg gaggcgctcc atggtgcccg
      601 gcgcgaagtt ctcgatcagc acgtcggccg tctcgaccag gtcgagcagc gcctgacgac
      661 cggcatcggt cttcaggttc agcacgacgc tgcgcttgtt ggaattgagc atcacgaact
      721 cgtgagactc gaccggctga tgcgagcgga agcgcaactg ttccccgccg gggggctcaa
      781 ttttgatcac gtcggccccc aactgtgcga gcagaagccc gcagtacggg ccgttgtaga
      841 tctgccccag ttcgaggacc cggatgccgt caaatgcctg tggcatgacc cgtccgatca
      901 cgagccgaca acgccggcgg cgccgccgtg gatgtagccc accggcccgg tgccctgtgc
      961 cgccgagacg gtttcggtca cgtgctgaat ggaggtcgcg tcgttgatgt gggccagcgt
     1021 gccgtcctgg ttgaatccga tttgcgcgcc ttccatccac agcagcacaa cggtgtcctc
     1081 ggtgttgtcc tcggggcagt agaaggtgtg caccgagccg ccgggctcat acaggtagga
     1141 cccagcggtc tgcggttgcg tcgggtactc ggcgtacttc cagcagcccg agaacgtgta
     1201 cacctcggcc gtgccggtgt ggtagtggat tggcaggagg caaccgggcg ccatcctggc
     1261 catgaggacc acctgtccct tttcggggtc caggcgcagc ggctggatgt ggataccggg
     1321 gcccatagcg tccttgagca gcggaatctg gtcgatgttg acggtcagga ggtcgtcctg
     1381 cggcaacgcg accaacggca gcgggacgcc gctgtcggtg cggaacatgc ctggggagac
     1441 aggtgcggtc atgattcgac cctttcgatc gtacgtattt acggattgaa atccgaatat
     1501 ccggatcaca tggctgactg tactgtgact cgcgcctcgt tgcaagtgcc gatccgcact
     1561 tgtattgatg ggcgtgctgg cctcgcgcac gcccacgcgc atcgcgcagt cgaccgaatg
     1621 atccacaccc ctcgagacat ccctgggcta tgaatcacgc aacgtggagg cggctcgtag
     1681 cacacgacgc ggcaagcgaa tcgtgaaggc atgccccgga ttcggggata ggtcttgccg
     1741 ccggtgacgc ttacgtggcg tccggcaacg acgctgagct gctcaagcga tggcgtgtgt
     1801 cgggttcacg cgtcggcgcg caggcggatg atcgggattg tgcgatggca ccgcgcggcg
     1861 cgccgccgat agtcaggcca gagtgggtag acccggtcgg ccatggccca gatgcgcttt
     1921 cgttcggcgg cgtcgctgac cgcactcgcg gtcagcaccg gtccgccgcc gttcgttgca
     1981 atctgcgcgc ggggattcgc gctgaggttg tggtaccagg cggggtgtct gtcggcgccg
     2041 tagttggagg cgatcacgat cacgtccggg ccgtcgtgaa agtagaagac tgggttggtg
     2101 cgcggttgac cggttttggc cccggtggtg gtgagcagcg ctgaaggcag catcagtccc
     2161 atggccagcc ggcccccggt ggctcgcagc agccacgggt cgacctttgg cgcgatgctc
     2221 ttcgcgagcc accggccgac gccggtccgc aggaaggcag cgtaggcgcg gacccggcgg
     2281 ctgacacttg cgggcggatt gacctgtggc agatcatcgg tcgcagtgct cggtgactcg
     2341 tcgcgcgtgg tcatggtgac gggcgccctt ctgcaacttg gtgatcgcga ccgtgtcctg
     2401 acggctcgaa gataatcacc tggcgcacgc catttcgctc catgtggctg tcatgcgccg
     2461 gccacatctc gataagcctc ggcatgttgc gcgcgacctc atcatctgta gccggtcgcg
     2521 acagataagt tgcagccgtg ccgccgatct cgatgcgtgc ctttggatcg gcgagcaggt
     2581 tcttcggcca actggaggct gcttccaacc cgaagttctc gcaagcggcg acgaggttct
     2641 taccatcgcg cacgtagtgc agcggaaccg ttcgatcttt gccggttctt ctgcctctcg
     2701 tcgtgagcag cattgtcggc agccggggac cactcatgga gaatcggcct cggctggcgc
     2761 ggatcagcac ccggtctacc cgggagccga catgcttcat gaacagggag aaccagccgt
     2821 agtggccgat ccgccgcatc acgcggagat acagcccata aggcccaccc gtcgtcggac
     2881 ctacgcgcgg ttggtcctcg tcagctgcgg tcatacatcc aacccttgtt cgtatttacg
     2941 tatgtatatc cagatatctg gatcacggcg tgactgtact gtgacccgcg cctcggtgca
     3001 agtccctgtc tgggggcctg tcgggcttgc taccgtctgc cgcatgcctg cccgtccgca
     3061 tcgcacggtc gaccgagtcg tagagatcct cgaaaccgtg tcgctgagcc agcgcggcgt
     3121 cactttggcc gaactggcaa ctgctatggg cgcggcgaag agctccgttc aggagctgac
     3181 caacggcctt cttgcgcgcg gctatctcat cgaagaggac cgccgtttcc acctcgggcc
     3241 gggcccgttc atcctggccg ctcgcgcaaa caagcttgcg gcgctctccc tcaatcatga
     3301 atttgtcgcc gagttgagca aggtgctcga ctgcactgtc ctggttgggg tgcgcgtcgg
     3361 cgactccatc gtcttcacag acttcgtggg cgaagagtca cccagccttt cgttcgttgc
     3421 gcgcacccac gcccgtcgcc ccctctatac cagcgccgcc ggaaagaccc tcctcgccaa
     3481 cgttcccgac gacgagatgt atcgtctcct cgatctcgtc agccccgagc aggcaggcga
     3541 agtccggcag tttcttgctg agcttcccga aatccgttcg cgccaattgg ctttcaaccg
     3601 tggtatcacg ttggcggacg cgtacgccgt ggccactccc ctgctggcac ccgacggcag
     3661 tctgatcgca gtgatcagtg cagccgtcga cgcatcacaa gcgaaccgtc tcgacgacat
     3721 cggtgaagag ctcaagaaca gcgttgcctc cctcggcccg aaatacgggc tcgacacacg
     3781 ctgaaacggg cgtatgacct cagatcacgc tctgcggcgc gcgtcgtcgc gctaactaac
     3841 ctgtcttcgg gttgacggct ttgaccgtca gccgcaacgc ctgtcgtcgg agcgcggcct
     3901 tggcgcggtc acgtccgccc accggtatta gtggcggcgc ggtgccgaga cgcggaaata
     3961 gcgcaagggc gtttgttctg tcgatcgctg cgcggctggc tgcgtcgagg ttcggatacg
     4021 tctcgaggcc cacggcaaag tactgaccgg ccgcggtggg cgcgaacggc cagtccgacc
     4081 cgaaggtgat gtgtcccggt ttcgcgaacg ccagcagtgt cggcagtgcg gcggcgctgg
     4141 acgacagcgc ggtgtcgaag tagaagctcg agaagtcgtc gagtgtgtcg gccgggctgg
     4201 caccggtctc ggccatgatc gcgacagcca tccggtgcga ggcgtacggg acgaagccgc
     4261 cggcgtggct gaggattaac ttgatgttcg ggtacttgcg ccgaataccg ttgcgcacca
     4321 acaggtacgc ggcgcgggtg gtgtccagca ggaagtcggc ggcgaacggg gcgacgccgg
     4381 gcacgctcgg gccgggcaga tcggccggat gaatgaacac cacggccgaa cgtgcatcga
     4441 gcgcggccca caggtcatcc tgaccgtcct cgccgaggta ggtgccgtcg ttgttggcca
     4501 gtagcacgac cccgtcggcg ttcagatcgt cgagtgcgcg cacggcctcg accacggatt
     4561 cgttgatgtg cggcatgggg acggtcgcga agaacccgaa ccgctccggc tgagatgcga
     4621 cgagatgggc gctgtaatcg ttcaggtcgc gggccagcgc gactgcatcg gccgggttgg
     4681 acaagaacgt cgtccctggg gtggaaaccg acagaatggc cgtggcgacg ccgagctcgt
     4741 ccatcgtttg cagcgacccc tccgggctcc agttgggcag agctcggccg ccggcttcat
     4801 cgatgcccgc ttggcgcaat atgtctcggt agtcgggcgg gatcatgtgg tggtgggtgt
     4861 caattcgcaa catcgatatc cctcgcggtc gttcggatga ctggcacggt acggccgacg
     4921 ccgaacgcgt ggacggccgt gcagaacagg tgctgatcgc tcacggcggc tgcgacctcg
     4981 gacgaggcga tgacgtcctc gacttcgtct cggcctctcg tgccgaagat gacggcgccg
     5041 tgatagcgcc gatgcggcgg gttgtcgtgg gctacgccga aggtcgagtg agccacgggc
     5101 gccaaggcca ggaagccgta ggtccgaaca tcacacgctc ccgcggcatg cagcgccggc
     5161 cccaagcgag tgtggacgaa cgcgcgaaaa gaccgtcc
//
LOCUS       DASRJA010000260         7715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3157810,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000260 DASRJA010000000
VERSION     DASRJA010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3157810"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="VFC01_11600"
     CDS             complement(<1..1299)
                     /locus_tag="VFC01_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HZN80316.1"
                     /translation="MSTPSVLMPLPVLIPMVGAALTLFAGRRPRLQRAIALAALCVVV
                     AVCAALVYLTDRDGTLVLQVGGWGPTESGMGPLGITLVVDRLSALMLVVSSIVLLCVV
                     FYAIGQGIRDGDERQPVSIFLPTYLVLSAGVCTAFLAGDLFNLFVGFEVLLSASFVLL
                     TIGASKDRVRAGIAYVMVSMVSSMVFLFGIALVYAATGTLNLAELAVRLDGVSAGTRT
                     ALFAVLLVAFGIKAAVFPLSTWLPDSYPTAPAPVTAVFAGLLTKVGVYAIIRTHSLLF
                     PTGGLDPLLLVAALLTMLIGILGAIAQSDVKRLLSFTLVSHIGYMVFGIALSSQLGMS
                     GAIYYVAHHIVVQTTLFLVVGLIERQAGASTMQRLGGLAAASPLLAFLFVVPALNLGG
                     IPPFSGFIGKVALLEAGAQNGSVLAWLLVGGGVLTSLLTLY"
     gene            complement(1296..1766)
                     /locus_tag="VFC01_11605"
     CDS             complement(1296..1766)
                     /locus_tag="VFC01_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit C"
                     /protein_id="HZN80317.1"
                     /translation="MTTYVVPLILIGALTSAGVYLLLERNMTRMLLGLLLIGNAINLL
                     ILTVGSPSGNPPIRGRTSNGETTTADPLAQGMILTAIVITMGIAAFVLALAYRSYRLT
                     TEEEVTDDPEDTRVSKLSAEEASALDVDRLRAEPARDTDLPDELDALPGMDGSR"
     gene            complement(1763..4648)
                     /locus_tag="VFC01_11610"
     CDS             complement(1763..4648)
                     /locus_tag="VFC01_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit A"
                     /protein_id="HZN80318.1"
                     /translation="MLAILFAHAVATALAPLLVYRWGRMAFYPLALVPLGSLVWVALN
                     WPGPGRATTANIEWLPELSMNITLRFDALAAIMSVLVLGIGALVLFYCADYFHHHDGH
                     TEKRLPSFAAELVAFSGAMFGLVCSDNMLVLYVFWELTTVLSFLLVGHYAERASSRRA
                     ATQALLVTTFGGLAMLVGIVVLGNVSGTYLLSELIAAPPTGIAASVGVVLVLIGALAK
                     SAIVPMHFWLPGAMAAPTPVSAYLHAAAMVKAGVYLIARMSPGFADSPPWRPTVVILG
                     VLTMLLAGWRAVREYDLKLILAFGTVSQLGLITIMVGAGGGDLMLAGLAMLCAHAMFK
                     ASLFMVVGVIDHATGTRDIRRLAWLGQRSRPLLIIAVGATASMAALPPFLGFVAKEAD
                     FETVAHAPSLGATAPYVLAGIVLGSVFTTIYSLRFLLGAFGRKGLPEPSKRVTEMHRT
                     ATTFLIPPAILAVAGLVFGVWPAGLDQVLDTYADTVPGSEKYQLALWHGFGAPLLLSA
                     LVLAVGTAAFFGRARLRRLRTARTPLGNADRAYDAALRGLDLVSVRLTASIQRGSIPA
                     TQSVILSTLVLVPVTVLALGARTRPEFALWDSPLQVVVGVLILAAALGATVMRNRLAA
                     VLLVGVTGYGCGAIFAFHGAPDLALTQFLVETLTLVIFVLVLRTLPAETDSANIKRYR
                     LPRAALALAVGAAVTTLAVFAMAARTGTPIAELLPDAAYFRGHGANTVNVLLVDIRAW
                     DTLGEISVLLVAATGVASMVFRHRRFGAAPRVADAGPSDTDRASAFTNHSPAVGDVTW
                     LRGSELRDPEHRSLVLEVATRMIFPLIMVLSAYFFFAGHNTPGGGFAGGLTAGLALVL
                     RYLAGGRYELGETLPLDAGKILGVGLGLSAGTAVASLLVGAPVLSSALVQIDVPVLGT
                     VKFVTALFFDLGVYLIVVGLVLDVLRSLGARVDVDVSERQPVEAPVR"
     gene            4745..6169
                     /locus_tag="VFC01_11615"
     CDS             4745..6169
                     /locus_tag="VFC01_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZN80319.1"
                     /translation="MDTADVVVVGSGPTGLMLACELALGGTTVRLLEERTDTPNITRA
                     FAVHARTLELLDARGLADDLLPRGVPVHEVAPPGGLELDLRELPTRFGMVLIVPQSGT
                     EKVLQARADELGVEIVRGAEVTGLTQDGDGVAVECASGETVRAPYVVGCDGAHSTVRR
                     LVGIDFVGKQYETHILLADVRLDRPPSETLAGVANEKGVVLVIPFGDGWFRAIVWDRL
                     REQAPLSEPVTEQEMRDAFGRIASDDYGMTEMRWSSRFLSERRQARHYRSGRVFLAGD
                     AAHVHSPLGGQGMNTGIGDAMNLGWKLAAAVRGSAPRWLLDSYEDERHRVGADVLRLT
                     DVFNQLVLGNSKPRRLLRSAVIGAVTRVPRGRRLLGERLSGIGIAYPRQRGDDWMVGR
                     RMPDIVCDGKRVYELLREAKFVLVTATPVKMDRPDIVHAVDNHPELPDAVLVRPDSYV
                     AWASERLPDASELAAAVNHWTRVT"
     gene            complement(6113..6586)
                     /locus_tag="VFC01_11620"
     CDS             complement(6113..6586)
                     /locus_tag="VFC01_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HZN80320.1"
                     /translation="MTASVTSVRAGDHDRERTAKQLGQALSQGYLAMDEYETRLQATF
                     ATPTTIELRQLVADLPLAQLRRNDPQRQAARRRAARLGVRIHFGAYLAMVVIVLTVWL
                     AVGLTAGDWYFWPVWPILGAGIGLASHAIPVRFAIPELCHARPMIDGRREFRSIR"
     gene            6635..7123
                     /locus_tag="VFC01_11625"
     CDS             6635..7123
                     /locus_tag="VFC01_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN80321.1"
                     /translation="MPAQLVSVKMLLNRIVTSTVISASREIAAPAEKIFELIADPSQQ
                     PRWDGNDNLAEAPAGQRVRTVGDVFTMTLTMGSDRTNHVVEFEEGRLIAWLPSEPGKA
                     PPGHLWRWELEPLDDSRTQVTHTYDWSALTDETRLPRARDTTADKLLASIERLAELAE
                     RG"
     gene            complement(7125..>7715)
                     /locus_tag="VFC01_11630"
     CDS             complement(7125..>7715)
                     /locus_tag="VFC01_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN80322.1"
                     /translation="VKGAELMDKWVGASEKAVRELFRRARDSAPSLVFLDEIDALAPR
                     RGQSFDSGVTDRVVAALLTELDGIEPLRDVVVVGATNRPDLIDPALLRPGRLEKLVFV
                     EPPDAEARREILRTAGKSIPLSDDVDLDALAGELDGYSAADCVALLREAALTAMRRSI
                     DAADVTAADVAKARESVRPSLDPVQVESLRAFSAAR"
BASE COUNT         1448 a         2540 c         2621 g         1106 t
ORIGIN      
        1 gtagagggtc aacaggctgg tgagcacgcc gccgccgacc agtagccacg ccaacaccga
       61 accgttctgc gcgcccgctt ccagcagggc caccttgccg atgaaccccg agaacggcgg
      121 aattccaccg agattgagcg caggcaccac gaacaggaac gccagtaacg ggctggccgc
      181 ggccaggccg ccgagccgct gcatcgtcga cgcacccgct tggcgttcga tcaggccgac
      241 caccaggaac agtgtcgtct gcaccacgat gtggtgtgcc acgtaataga tggcccccga
      301 catgccgagc tgactcgaca gcgcgatgcc aaacaccatg tagccgatgt ggctgacgag
      361 cgtgaacgac agcagacgtt tgacgtcgct ctgggcgatt gcgccgagga tgccgatcag
      421 cattgtcagc agcgcagcca caagcaatag cgggtcgagc ccaccggtgg ggaacagcag
      481 cgagtgtgtc cgaatgatcg cgtacacacc gactttcgtc agcaggccgg cgaacaccgc
      541 ggtgactggc gccggcgcgg tgggatacga gtccggcagc cacgtcgaca acgggaacac
      601 tgcggccttg atgccgaatg cgaccaggag caccgcgaac aacgcggtgc gggtgcccgc
      661 cgacactccg tcgagtcgca cggccagttc ggcgaggttc agcgttccgg tggctgcgta
      721 tacgagcgcg atgccgaaca ggaacaccat cgacgacacc atcgagacca tcacgtaggc
      781 gatgcccgcg cggacgcggt ccttgctggc accgatcgtc agcagcacga aactcgccga
      841 caacaggacc tcgaacccga cgaacagatt gaacaggtcg ccggccaaga atgccgtgca
      901 aacgccggcc gacagcacca ggtaggtggg caggaagatc gagaccggtt gacgctcatc
      961 gccgtcgcgg atgccctgcc cgatcgcgta aaagaccacg cacagcaaca caattgacga
     1021 caccacgagc atcagcgcag aaagtcgatc caccaccagt gtgatgccga gcgggcccat
     1081 accggattcc gtcggccccc aaccgccgac ttgcagcacc agcgtgccat cgcggtcggt
     1141 gaggtagacc agcgcagcgc acaccgcgac caccacacac agcgcggcca gggcgatggc
     1201 ccgctgcaac cggggtctgc ggccggcgaa caatgtcagc gcggcgccga ccatcgggat
     1261 cagcaccggc agcggcatca ggacggaagg ggtgctcatc gcgagccgtc catccccggc
     1321 agtgcgtcga gctcgtcggg caggtcggtg tcgcgggccg gctcggcgcg cagacggtcc
     1381 acatcgagcg ccgaggcctc ctccgcagac agcttcgaga ccctggtgtc ctcggggtcg
     1441 tcggtgacct cctcctctgt ggtcagccga tatgaccggt aggcgagtgc cagcacgaac
     1501 gccgcgatac ccatcgtgat gacgatggcg gtcaggatca tgccctgcgc caacggatct
     1561 gccgtggtgg tttcaccgtt gctggtcctg ccccgtatcg gcgggttgcc ggatggactg
     1621 ccgacggtca ggatcagcag gttgatcgcg ttgccgatca gcaacagtcc cagcaacatt
     1681 cgcgtcatgt tgcgttcgag cagcaggtag acacccgcgc tggtaagcgc tccgatgagg
     1741 atcaggggaa ctacataagt cgtcatcgca ctggagcctc caccggctgc cgctcgctca
     1801 cgtccacgtc cacccgcgcg ccgaggctgc gcagcacgtc gagcacgagg ccgacaacga
     1861 tcagatacac accaaggtcg aagaacaacg cggtgacgaa cttgacggtg cccagcaccg
     1921 gcacatcgat ctggaccaat gccgacgaca acaccggtgc gccgaccagc agcgacgcca
     1981 ccgcagtacc ggcggacagc ccgagcccca cgcccagaat ctttccggcg tccagcggca
     2041 gcgtctcgcc gagttcgtaa cggccgccgg ccaggtagcg cagcaccaat gcgagacccg
     2101 cggtgagccc gcctgcgaag ccgcctcctg gggtgttgtg tcccgcgaag aagaagtacg
     2161 ccgacaacac catgatgagc gggaagatca tgcgggtggc gacctcgagc accagagacc
     2221 tgtgttccgg gtcgcgcagt tcgctgccgc gcagccaggt gacgtcgccg actgccgggc
     2281 tgtgattggt gaaggcggag gcccggtcgg tgtccgaggg gccggcatcg gcgacgcgcg
     2341 gcgccgcccc aaaacgcctg tgccgaaaca ccattgaggc gacgccggtg gcggcgacca
     2401 gcagcacaga aatctcgcca agggtgtccc aggcccggat gtccaccagc agcacgttga
     2461 cggtgttggc gccatgtccg cggaaatacg ccgcgtcggg cagcaattcg gcaatcgggg
     2521 taccggtgcg ggccgccatc gcgaacaccg ccaacgtcgt caccgctgcg ccgacagcca
     2581 gcgccaacgc cgcgcggggc agccggtacc gcttgatgtt cgcgctgtcg gtttccgcgg
     2641 gaagcgtgcg cagcaccagc acgaagatca ccagagtcag tgtctccacg agaaactggg
     2701 tgagcgccag atccggtgcg ccgtggaagg cgaagatcgc gccgcagccg tagccggtca
     2761 cgccgaccaa cagcaccgcc gcgagccggt tacgcatcac ggttgcgccg agcgccgccg
     2821 ccaggatcag caccccaacc acgacctgca atggagagtc ccacaaagcg aactcgggac
     2881 gggtgcgggc cccgagcgcg agcacggtca ccggcaccaa caccagtgtc gacaggatca
     2941 ccgattgtgt cgccgggatc gaaccgcgct gtatggatgc ggtgagccgc acggacacaa
     3001 ggtcgaggcc gcgcagtgcg gcgtcgtagg cgcggtcggc attgcccaac ggcgtgcgcg
     3061 ccgtgcgcag ccgacgcagt cgggcccggc cgaagaacgc cgccgtgccg acggccagga
     3121 ccagcgccga caacagcagc ggtgcaccga atccgtgcca cagtgcgagt tggtacttct
     3181 cgctgccggg taccgtgtcg gcataggtgt ccagtacctg gtccagtccg gcgggccaca
     3241 cgccgaacac caagcccgcg acggccagga tcgccggcgg aatcaggaaa gtggttgccg
     3301 tgcggtgcat ttcggtgacc cgcttgctgg gctccggcaa tcccttgcgg ccgaacgccc
     3361 ccagcaggaa ccgaaggctg tagatggtgg tgaacaccga cccgagcacg atgcctgcga
     3421 ggacgtaggg cgcagtggcg ccgagcgacg gtgcgtgcgc cacggtttcg aagtcggcct
     3481 cctttgccac gaagccgagg aacggcggca gcgcggccat gctcgcggtc gcgccgaccg
     3541 cgatgatcag caggggtcgg ctgcgctgtc cgagccaggc cagcctgcgg atgtcgcggg
     3601 tgccggtggc gtggtcgatg acgccgacga ccatgaacag cgacgccttg aacattgcgt
     3661 gcgcgcacag catggccagc ccggcgagca tcagatcgcc gccgccggcc ccgaccatga
     3721 tcgtgatcaa cccgagctgg ctgaccgtgc caaacgcgag gatcaacttc aggtcgtact
     3781 cgcgcacggc ccgccagccc gccaacaaca tggtgagcac gccgaggatc accacggtcg
     3841 gccgccacgg tggtgagtcg gcgaagcccg gactcatccg cgcgatgaga tagacgccgg
     3901 ccttgaccat cgccgcggcg tgcaggtaag cgctgaccgg tgtcggggcg gccatcgctc
     3961 cgggaagcca gaaatgcatt ggcacgatcg ccgatttggc gagtgcgccg atcaacacca
     4021 gcaccacgcc aaccgatgcc gcgatacctg tcggcggcgc cgcgatcagt tcggacagca
     4081 gatacgtgcc tgacacgttg ccgagcacga cgatgccaac cagcatggcc agcccgccga
     4141 acgtcgtcac caacaaggcc tgagtcgcag cgcggcggct gctggcgcgt tcggcgtaat
     4201 ggcccaccaa cagaaatgac aggaccgtgg tgagctccca gaacacgtag agcaccagca
     4261 tgttgtcgct gcagacaagc ccgaacatgg cgccggagaa cgcgacgagc tcggcggcga
     4321 aactgggcaa ccgcttctcg gtgtggccgt cgtggtgatg gaagtagtcg gcgcagtaaa
     4381 aaaggaccag tgcgccgatc ccgagcacca gcacgctcat gatcgccgcg agcgcgtcga
     4441 accgcagcgt gatgttcatc gacagctcgg gcagccattc gatgttggcc gtcgtcgccc
     4501 ggcccggccc cggccagttc agcgccaccc agaccagcga acccagcggc accaacgcaa
     4561 ggggatagaa cgccatccgg ccccaccgat agacgagcag cggggcgagc gccgttgcga
     4621 cggcatgggc gaacaggatg gcgagcatgg cactccgatc gttttggggt cggggtgtca
     4681 gccgggttaa gggctttcgt ttaacgccag tctacgtggg cggatgcagc taccatccgg
     4741 cctcgtggac actgcggacg tcgttgtggt cggttccgga cccacgggat tgatgctggc
     4801 gtgcgagctt gcgcttggtg gcaccacggt gcggctgctc gaggagcgca ccgatacgcc
     4861 caacatcaca agggcattcg cagttcatgc ccggacactg gagctgctcg acgcgcgcgg
     4921 tctggccgac gacctgctgc cgcggggcgt tccggtgcac gaggttgcgc cgccgggcgg
     4981 gttggagctg gacctgcgtg agctgcccac ccggttcggg atggtgctga tcgtcccgca
     5041 aagcggaacg gagaaggtgc tacaggcccg cgcggacgag ctcggcgtcg agatcgtccg
     5101 cggcgccgag gtgaccggac tgacgcagga cggcgatggg gtggcagtcg agtgcgccag
     5161 cggtgagaca gtgcgtgcgc cgtacgtggt tggctgcgac ggcgcgcaca gcactgtgcg
     5221 acggctggtc ggcatcgact tcgtcggcaa gcagtacgag acgcacatcc tgctggcgga
     5281 cgtccgtctg gatcggccgc catcagagac gttggccggc gtcgccaacg agaagggggt
     5341 ggtgctcgtc atcccgttcg gcgacggctg gtttcgagcc attgtgtggg accgcttgcg
     5401 ggaacaggcg ccgttgtccg aaccggtcac tgagcaagag atgcgcgatg cgttcggccg
     5461 catcgcgagc gatgactatg gcatgaccga gatgcgctgg agctcaaggt ttctcagcga
     5521 gcggcggcag gcacggcact accggtccgg gcgggtgttt ctggcgggcg acgccgccca
     5581 tgtgcactcg ccgctcggcg ggcagggcat gaacaccgga atcggtgacg cgatgaacct
     5641 gggctggaag ctggccgccg cggtgcgcgg atccgcgcca cgctggctgc tggacagcta
     5701 cgaggacgag cgccaccgcg tcggcgccga cgtcctgcgc ttgaccgacg tgttcaacca
     5761 gctggtgctc ggcaactcca agccacggcg gctactgcgc agcgccgtga tcggagccgt
     5821 gaccagagtg ccccgcggca ggcggctctt gggcgaacgg ctcagcggca tcggaatcgc
     5881 gtatccgcgc cagcgcggtg acgactggat ggtcggccgg cggatgcccg acatcgtgtg
     5941 cgacggcaag cgggtatatg agctgctgcg cgaagccaag ttcgtgttgg tgacggccac
     6001 gccggtgaaa atggatcggc ccgacatcgt gcatgcggtc gacaatcacc cggagttgcc
     6061 cgacgccgtg ctcgtccgtc ccgacagcta tgtcgcgtgg gcgagtgaac ggctaccgga
     6121 tgcttctgaa ctcgcggcgg ccgtcaatca ttggacgcgc gtgacatagc tccggaatcg
     6181 cgaagcggac gggaatcgcg tgggacgcca gaccgatgcc cgcgccgagg atcggccaga
     6241 ccggccagaa gtaccagtcg cctgcggtca ggccgaccgc cagccacacc gtcagcacga
     6301 tgacgaccat cgccaggtaa gcgccgaaat gaatccgcac gccgagccgt gcggcgcgcc
     6361 gtcgggcggc ctggcgctgc ggatcgttgc ggcgcagctg cgcgagcggc aggtcggcga
     6421 cgagttggcg cagctcgatg gtggtggggg tggcgaacgt ggcctgcagc cgggtctcgt
     6481 actcgtccat cgcgaggtag ccctgcgaca gtgcctggcc cagctgcttg gcagtccgct
     6541 cgcggtcgtg atctccggcg cggaccgatg tgactgatgc ggtcatggag cctccgatgc
     6601 ttaatcttga cagtggcaag ttccgtaccc gactatgccc gcccaacttg tcagtgtcaa
     6661 gatgttgttg aatcggatcg tgacttcgac cgtcatcagt gcgagccgtg agatcgcggc
     6721 accggcagaa aagatcttcg aactcatcgc cgatccgtcg cagcagccgc gatgggatgg
     6781 caacgacaac ctcgccgaag cgcctgccgg ccagcgtgtg cggacggtgg gtgatgtgtt
     6841 cacgatgacg ctcaccatgg gcagcgatcg gacgaaccac gtcgtcgaat tcgaggaggg
     6901 ccgactcatc gcgtggctgc cctcggagcc gggtaaggca ccgcccggtc acctgtggcg
     6961 atgggagctc gaaccgctcg acgattcgcg cacgcaggtg acccacacct acgactggtc
     7021 ggcactcaca gacgagacgc gactgccacg cgcgcgcgac accacggcgg acaaactgct
     7081 ggcgtcgatc gagaggctcg cagagctggc cgagcgcggc tgagtcagcg cgcggccgag
     7141 aacgcccgca gtgactcgac ctggacgggg tccagcgagg gccgcacgga ttcacgggct
     7201 ttggcgacgt cggcggcggt gacgtcggcc gcgtcgatcg agcggcgcat cgcagtcaat
     7261 gcggcctcgc gcaacagcgc cacgcaatcc gccgcgctgt aaccatccag ctctcccgcc
     7321 aacgcatcca ggtcgacgtc atcgctgagc gggatggatt tgcctgcggt gcgcaggatt
     7381 tcgcggcggg cctcggcgtc gggcggctcg acgaacacca gcttttcgag tcggcccggt
     7441 cgcagcagcg cagggtcgat caggtcgggc cggttggttg cgccgaccac caccacgtcg
     7501 cgaagcggtt cgatgccgtc gagttcggtc agcagcgcgg caaccacgcg gtcggtgaca
     7561 ccggagtcaa agctctgccc gcgtcgcggt gccagcgcat cgatctcgtc gaggaacacc
     7621 agcgacggcg ccgaatcgcg ggcgcgccgg aatagttcgc gcaccgcctt ttccgaggca
     7681 cccacccact tgtccatcag ctcggcgccc ttgac
//
LOCUS       DASRJA010000261         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_702163,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000261 DASRJA010000000
VERSION     DASRJA010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702163"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="VFC01_11635"
     CDS             complement(<1..1315)
                     /locus_tag="VFC01_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HZN80323.1"
                     /translation="MARPASEPLTDIPPAARDTVSGLLAEPTVPVKVLVSGGIGTGKS
                     SVLAAIRSALRAAGVKVLTRAPRDGDESATVVIDDAHLLDDAELDRLGELAADSASTV
                     VVATEPLAHRPALRSLAIAIERENPVVSLGGLSPAEVNRVAAETLGAPPTSEIIRALM
                     VATAGLPFLLQPAIAAAGSPDGEPPATAIMQAAKFALIERLRRLEEPVLDTLLVSSLS
                     PELGSDDVAAALRVGADEAQALVDRARASGLIEPSHSHAFLRSVHRCIAGIIGTARHH
                     DTELSLLVSQLESSTLSADLALRMAEHGLHDDRLATALADLAARTRGQPARAARLYRA
                     AADAGATALSAQLADALALTGDCTTAGRLADELLGSDDAAERAAAVRIAGSIAMHDGS
                     AAQAADLFRWLGPYPDAFVSAAGTIVTVAAGDLEAAKAALNAESAG"
     gene            complement(1319..>2872)
                     /locus_tag="VFC01_11640"
     CDS             complement(1319..>2872)
                     /locus_tag="VFC01_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZN80324.1"
                     /translation="SGERVLVEALDAMAHTVGGGAPIAIAVPAHWGPGTVGALRGALR
                     AKPSLAPNGVPAALIPDSAAAFAALQAAPGLPSSGVVALVDLGGSGTSITLADAGSNF
                     DQIGETVRYADFSGDQIDQALLNHVMAGIADANNADPTGTLGLGSLARLRDEARQAKE
                     RLSADTATVVHAELPGFNSDVRVTRNELEGLIAEPLAGLLNTIQETLQRNNIPAANVS
                     AVATVGGGANIPRVTQQLSERLRAPVVTTPQSQLNVAAGAALVADRGLEADAPTGMAP
                     TGLAPAADAPTGLSAAGWAAGAAGLAASESASDGTASATFRALAWSQDESPGGEPVPY
                     SGEDYTFDQPTDARPQMAFAHEEEGYEPEPGPLPWYKRPPILFGAAAAAVLLAIGGLA
                     VTLTSTSGSSGPVTETATQVETGPSPTEPATEGSPVTVTVTGPNGEPSTTVVPSPTPS
                     SSATTTSPTTTTTTTTTTTTTTTTTTTTTTPPTTTTTKPTTTQPTTTQPPVTTTTAAA
                     LSPATSTEP"
BASE COUNT          370 a          956 c         1078 g          468 t
ORIGIN      
        1 gccccgcgct ctctgcgttc agtgcagcct tggcggcctc gaggtcaccc gctgcaaccg
       61 tgacgatcgt gcctgccgcg ctgacgaacg cgtcgggata tggcccgagc caacggaata
      121 ggtccgcggc ctgcgccgcg ctgccgtcgt gcatggcgat gctgccggcg atccgcaccg
      181 cggccgcgcg ttcggcggcg tcgtcggagc cgagcagctc gtcggccagc cggcccgcgg
      241 tagtgcagtc accggtcagc gcgagcgcgt cggccagctg cgcggacagg gccgtcgctc
      301 ccgcatcggc ggcggcgcga tacaaccgtg ccgcgcgtgc cggttggccg cgggtgcggg
      361 ccgcgaggtc ggcgagcgcg gtggccagcc gatcgtcgtg cagcccgtgc tcggccatcc
      421 gcaacgcgag atctgctgac agcgttgatg attcgagctg tgacaccagc agggagagtt
      481 cagtgtcgtg gtgacgcgcc gtgccgatga tccccgcgat gcaccggtgc accgaccgca
      541 ggaaggcgtg gctgtgcgac ggctcgatca ggccgctggc gcgggcgcgg tccacgaggg
      601 cttgcgcttc gtccgcgccg acccgcagtg cggcggcgac atcgtcggac ccgagctccg
      661 ggctcagcga cgacaccagc agcgtgtcga ggaccggctc ttcgagccga cgcaaccgtt
      721 cgatcaatgc gaacttcgcc gcttgcatga tcgccgttgc cggcggctcg ccatcgggtg
      781 agcccgccgc agcgatcgcc ggctgcagca ggaacggcaa gccggccgtc gccaccatca
      841 gcgcccgaat gatctcggaa gtcggtgggg cgccgagtgt ttcggctgca acacggttga
      901 cctccgccgg gctgagcccg ccgagcgaaa ccacaggatt ttcgcgctcg atggcaatgg
      961 caagcgaccg cagcgctggg cgatgggcga gcggttcggt ggcgacgacg actgttgacg
     1021 cgctgtcggc ggccagctca ccgagccgat cgagttccgc gtcgtcgagc agatgcgcgt
     1081 cgtcaatgac gacggtcgcc gattcatcgc cgtctctcgg cgccctcgtc aacactttga
     1141 ccccggctgc gcgcagcgcg gaacggatgg ctgcgagaac cgaactctta ccggtgccaa
     1201 tgcctccgga gaccagaacc ttgaccggga cggtcggctc ggcaagcagc cctgacacgg
     1261 tgtcgcgtgc ggccggcggg atgtcggtca gcggttcgga cgccggtctg gccatgggtc
     1321 acggctctgt gctcgtcgcc ggcgacagcg cggcggcagt ggtcgtggtc accggcggct
     1381 gtgtagtcgt cggctgggtg gtggtgggct tcgtcgtcgt cgtggtcggc ggcgtcgtgg
     1441 tggtcgtcgt ggtcgtggtc gtggtggtcg ttgtcgttgt cgttgttgtc gtcgtggtcg
     1501 gcgatgtggt ggtcgcgctg gacgatggtg tcggcgacgg caccaccgtc gtgctcggct
     1561 caccgttggg gccggtcacc gtcactgtca cgggggagcc ctcggtggca ggttcggtgg
     1621 gcgacgggcc cgtctcgact tgggtggccg tctcggtgac cgggccgctg cttccgctgg
     1681 tgctggtcag ggtgaccgcc agcccgccga tggccagcag cacggccgcg gccgccgcgc
     1741 cgaacaggat gggcggacgc ttgtaccagg gcagcggccc aggctcgggc tcgtaaccct
     1801 cttcctcgtg cgcgaacgcc atctgcgggc gagcgtcggt gggttggtcg aaggtgtagt
     1861 cctcgccgga gtacggcacc ggctcgccgc ccggtgattc gtcctgcgac caggccagcg
     1921 cacggaacgt cgcagacgcg gtgccgtcag aggccgattc ggaggcggcc aatccggcgg
     1981 cgcctgcggc ccatcccgct gcggacagac cggtgggagc gtcggcggcg ggcgccagcc
     2041 ccgtcggcgc catcccggtg ggagcgtcgg cctccagccc gcgatcggcc accagtgcgg
     2101 caccggctgc cacgttgagc tgtgactgtg gtgtggtgac gacgggtgcg cgcagccgtt
     2161 ccgacagctg ctgggtgacc cgcggaatgt tggccccgcc gccaaccgtc gcgacggcag
     2221 acacattggc cgcgggtatg ttgttgcgct gcaaggtttc ttggatggtg ttgagcagac
     2281 cggccagcgg ctcggcgatg aggccctcga gctcgttgcg ggtcaccctg acgtcggagt
     2341 tgaaaccggg tagctcggcg tgcaccacgg tcgcggtgtc cgcggacaac cgctccttgg
     2401 cctgccgggc ctcgtcgcgc agccgggcca gcgatccgag tcccagcgtg ccggtgggat
     2461 cggcgttgtt ggcgtcggcg attccggcca tcacgtgatt gagcagagcc tggtcaatct
     2521 ggtcgccgga gaagtcggcg tagcggacgg tttcgccgat ctggtcgaag ttcgaaccgg
     2581 cgtcggccag cgtgatgctg gtcccgctgc ctccgaggtc caccagcgcg acgacgccgc
     2641 ttgacggcag ccccggggcg gcctgcaggg ccgcgaacgc cgcggccgaa tcgggtatga
     2701 gcgccgcggg caccccgttc ggcgcaaggc tcggtttcgc gcgcagtgcg ccgcgcagcg
     2761 ctccgacggt tcctgggccc cagtgcgcgg gcacggcgat cgcgatggga gcgccgccgc
     2821 caacggtgtg tgccatcgcg tcgagggctt cgacaagtac gcgctcgccg ct
//
LOCUS       DASRJA010000262         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_54181,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000262 DASRJA010000000
VERSION     DASRJA010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54181"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..912)
                     /locus_tag="VFC01_11645"
     CDS             complement(139..912)
                     /locus_tag="VFC01_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZN80325.1"
                     /translation="MCDFELAVCSEMVFMDLPIIDRVRRIAELGFAVEIWSWHDKDLQ
                     ALADTGARFSSMTGYLHGDLIDPTTADDVVRTAELSIKAAETLGVPRLNLHTAELVDG
                     KAARPRYRSTGEMWATAMRTLERIGELGAATGVTFCVENLNTIVDHPGVPLARAKDTL
                     ALVEAIGHPNVKMMLDLYHAQIGEGNLIELVRRCGDAIGEIQVADVPGRCEPGTGEIN
                     YPAIAKALRDMGFAGTVGLEAWASGDSVTALEAFRAAFS"
     gene            1070..2059
                     /locus_tag="VFC01_11650"
     CDS             1070..2059
                     /locus_tag="VFC01_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZN80326.1"
                     /translation="MRFSRLAAIAGASVLVVGIASCSGTGGKPQETGGGMGAGQASTP
                     RATVAMITHEVPGDSFWDLIRKGAQAAADKDNIELRYSNDPEAPNQANLVQSAIDSKV
                     NGIALTLAKPDAMAPAVKAALAANIPVVAFNSGFDNFKAMGVQEYFGQDEKLAGVAAG
                     ERLNKDGAKKVICVIQEQGQVALESRCAGVTQGFKGQTEILNVNSKDMPSVESTITAK
                     LQQDPSVDHVVALGAPIALAAVQSVGNAGSSAKVVTFDTNAALVDAIKGGKVLWAVDQ
                     QPFLQGYLAIDSLWLYLNNKNLIGGGQATLTGPSFIDQTNIDAVAELAKSGTR"
     gene            2085..3143
                     /locus_tag="VFC01_11655"
     CDS             2085..3143
                     /locus_tag="VFC01_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN80327.1"
                     /translation="MTTQTDLDVASHKVVRDERVKERNRLQRMLIRPEMGAAIGAVAI
                     FVFFLIVAPPFRSPEALATVLYASSTIGIMACAVALLMIGGEFDLSSGVAVTTSSLAA
                     SMLAYNLHLNLWAGAALALIVALAVGFFNGYLVMKTKIPSFLITLGTLLMLTGINLAV
                     TKLISGQVATPSVNDMAGWDSAQKVFSSSFTIAGVSVRITVLWWLLFTAVATYVLFKT
                     KVGNWIFAVGGDQESARAVGVPVTAVKVGLFMFVGFCAWFAGMHLLFAFNTVQSGQGV
                     GNEFIYIIAAVIGGCLLTGGYGTAIGAAIGAFIFGMVQQGIVYAGWNPDWFKFFMGVM
                     LLFAVIANNAFRNYAAKR"
     gene            3148..>3482
                     /locus_tag="VFC01_11660"
     CDS             3148..>3482
                     /locus_tag="VFC01_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN80328.1"
                     /translation="MTATVDRPVEEAPSGGKVPLVEMKNIGKSYGNITALKDISLRVH
                     AGEVTGILGDNGAGKSTLIKIIAGYHPQTDGELLVDGEATHFNSPKDALAKGIATVYQ
                     NLAVVPLMPV"
BASE COUNT          657 a         1068 c         1089 g          668 t
ORIGIN      
        1 ggtggaccgt taacacggcg ccgatccacc tggggcactg gtggtgcgtc gccgcggtcg
       61 cctaaccttt cctcgcgcga gcagacgcaa aactgccgta tttcgccgcg aaatgggcag
      121 ttttgcgtct gctcgccact aactgaacgc cgcgcggaac gcctctaatg ccgtgacact
      181 gtccccggac gcccaggcct ccagaccgac ggtcccggca aagcccatgt cccgcaacgc
      241 cttcgcgatc gccgggtagt tgatctcgcc ggtgcccggc tcgcagcggc ccggcacgtc
      301 ggcgacctgg atctcgccga tagcatcgcc gcatcgccgc accagctcga tcagattccc
      361 ctcgccgatc tgggcgtgat acagatcgag catcatcttc acgttgggat ggccgatggc
      421 ttccaccaat gccagcgtgt ccttggcacg ggccagcggg acgccgggat ggtcgacgat
      481 ggtgttgagg ttctccacgc agaatgtgac gcctgtcgcg gcgccgagct cgccgatgcg
      541 ctccagtgtg cgcatcgccg tcgcccacat ctcgccggtc gaccggtagc gcggccgcgc
      601 cgccttgccg tcaaccagct ccgccgtatg caggttgagc cgtggaacgc caagcgtctc
      661 agcggctttg atagacagct ccgccgtccg gaccacgtcg tcggccgtgg ttggatcgat
      721 gaggtcgccg tgcagatagc ccgtcatcga ggagaaccgg gcaccggtgt cggccagcgc
      781 ctgtaggtcc ttgtcgtgcc agctccagat ctcgaccgca aagccgagct cggcgatgcg
      841 ccgaacccgg tcgatgatcg gcaggtccat gaagaccatc tcggaacaga ccgcgagctc
      901 gaagtcgcac attggtttgt cacctccctc gctctgacca gcatggacgc acattagcag
      961 tttgatatgc agtcctaatg tccgaacaaa gtattgactt tcaggtgtga gccgtattac
     1021 atcgagcttg agtggcgtgt cacgccgcac tcgagagcaa ggagaagcaa tgaggttcag
     1081 ccggctcgcg gcgatcgccg gagcgagcgt gctcgtggtt ggaatcgcgt catgttcagg
     1141 caccgggggc aagccccagg aaaccggcgg gggaatgggc gccggccagg caagcactcc
     1201 gcgcgccacg gtcgcgatga tcacccacga ggtgccaggc gactcgttct gggacttgat
     1261 ccgcaagggc gcccaggccg cagccgacaa ggacaacatc gagctgcgct actccaacga
     1321 tccggaggcc ccgaaccaag cgaatctggt tcaaagtgcc atcgacagca aggtcaacgg
     1381 aatcgccctg acactggcca agccggacgc catggcgccc gcagtcaagg ccgcccttgc
     1441 agcgaacatc ccggttgtgg cattcaactc gggcttcgac aacttcaagg cgatgggcgt
     1501 gcaggagtac ttcggtcaag acgaaaagct cgccggggtc gccgcgggag aacgactcaa
     1561 caaggacggc gccaagaagg tgatctgcgt catccaggaa cagggtcagg tcgcgcttga
     1621 atcgcgatgc gccggcgtca cccagggatt caagggtcag accgagatcc taaacgtcaa
     1681 cagcaaggac atgccgtctg tcgaatcgac catcacagcg aagcttcagc aggatccctc
     1741 cgtcgaccac gtcgtcgcgc tgggcgcgcc gattgcgttg gccgcggtgc agtctgtcgg
     1801 caatgccggt agctccgcga aggtcgtcac cttcgatacc aatgcggcgc tggtcgacgc
     1861 gatcaagggc ggcaaggtgc tgtgggctgt ggatcagcag ccattcctgc aggggtatct
     1921 ggcaatcgac tcactgtggc tgtatctcaa caacaagaac ctgatcggtg gcgggcaagc
     1981 caccctgacc ggcccgtcgt tcatcgacca gacgaacatc gacgccgtcg ccgagttggc
     2041 caagtccggg acccgttaag ccgccgccca ggagagagcg aatcatgact acacaaactg
     2101 atctggacgt tgccagtcac aaagttgttc gcgacgaacg cgtcaaggag cgaaaccgtc
     2161 tgcagcgcat gctcattcgt cccgagatgg gtgcggcgat cggcgcggtc gcgatctttg
     2221 tcttcttctt gatcgttgcc ccgccgttcc ggtcacccga ggccctggca actgtgctgt
     2281 atgccagctc gacgatcggg atcatggcct gtgcggtcgc gctactgatg atcggcggcg
     2341 agttcgacct gtcctcgggt gtggccgtaa ccacgagttc gctcgccgcg tcgatgctcg
     2401 cttacaacct gcatctgaac ctctgggcgg gggcggcgtt ggccctgata gtcgccctgg
     2461 ctgtcggatt tttcaatggg tatttggtga tgaagaccaa gatcccgagc tttttgatca
     2521 cgttgggcac cctgctgatg ctcaccggca tcaatctcgc cgtgaccaaa ctcatttcgg
     2581 gtcaggtcgc gacgccgagc gtgaacgaca tggctggctg ggattcggcg cagaaggtgt
     2641 tctcgtcgtc gttcaccatt gccggtgtca gcgtccgtat cacggtgctg tggtggctgt
     2701 tgttcaccgc cgtggcaacc tatgtgctgt tcaagacgaa ggtcggaaac tggatattcg
     2761 ccgtcggcgg tgatcaggaa agtgcccgcg cggtcggtgt gccggtgacg gccgtgaagg
     2821 tcgggctgtt tatgttcgtc ggcttttgtg cctggttcgc cggcatgcat ctgctgttcg
     2881 cgttcaatac cgtgcagtcc ggtcagggtg tcggcaacga attcatctac atcatcgccg
     2941 cagtgatcgg cggctgcctg ctcaccggcg gatacggcac ggcgatcgga gcggcgatcg
     3001 gcgccttcat tttcggcatg gtgcaacagg gcattgtgta tgccggttgg aaccccgact
     3061 ggttcaagtt cttcatgggc gtgatgctgc tgttcgcggt gatcgccaac aacgcattcc
     3121 gcaactacgc cgcgaagagg tgaccccgtg accgcaactg ttgaccgtcc cgtggaagag
     3181 gctccgtccg gcggcaaggt gccgctcgtc gagatgaaga acatcggaaa gtcctacggc
     3241 aatatcactg cgctgaagga tatttcgctg cgggtgcatg ccggcgaagt gaccggcatc
     3301 ctcggtgaca acggtgcagg caagtcgacg ttgatcaaga tcatcgccgg atatcacccg
     3361 cagaccgacg gcgaacttct ggtcgacggc gaagccacgc acttcaattc gccaaaagac
     3421 gcattggcca agggcattgc gacggtgtat cagaacctcg ctgtcgtgcc gttgatgccg
     3481 gt
//
LOCUS       DASRJA010000263         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2721718,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000263 DASRJA010000000
VERSION     DASRJA010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2721718"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1758
                     /locus_tag="VFC01_11665"
     CDS             205..1758
                     /locus_tag="VFC01_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HZN80329.1"
                     /translation="MQGRSRPDRELLDAQALVGHLVPAGSVYAFLAEHRATVFPDELF
                     ADLFASGRGRPSVPGPVIASVLVLQALQGLSDRDAAEALRCDLRWKVACGLPLDDEGL
                     HATTLTYWRARLRASDAPQRIFDAVRGVINATGVLKGRDKRALDSVVLDDAVATQDTV
                     TQLIAAIRRVRRDVPGAAGFIAEHCHAHDWDDLAKPRIDWDDTAARDALITALVTDAL
                     GILDTFADANLAGDERAGQALALLALIAGQDVEPADDSDGSDGRWRIARRVAPDRVIS
                     TVDTETRHVHKTVHQRQDGFKGHVAVEPETGIFTHVQLAKATGDDNHEAVIGLTLIAS
                     EPAGTEVLGDSAYGTGDMRAALAEAGQVAVIKPMPLRAAVPGGFTIDDFAVDEDNQQV
                     TCPGGHTRHITPNRHVVFGVVCASCPLRERCTTSKDGRTLRLHPHDALLRAARRSWAT
                     DEALREAYRQHRPMVERSIAWLIGTRSRCRRLPYRGVTANNWWLHTRAAALNLRRLLN
                     LGLAADNGA"
BASE COUNT          327 a          707 c          694 g          297 t
ORIGIN      
        1 gcgcgaaaat tcacgggcac catcttgccg tacgcgcctt cgagggtatc gccagcgccg
       61 ccaagatcat gatctggtgg tcaccgaatc gcgccgatca tgaaccgtag ttcatgatcg
      121 gcgcgattcg gggcgtgcta ggagactgct gaacaatccg gcgtgtcggt tgccttcgct
      181 ttgatggtgg tcgatcatga tcgggtgcag ggaaggtcgc gcccggatcg ggagttgttg
      241 gacgctcagg cgttggtggg tcatctggtg ccggccgggt cggtgtatgc gttcctggcc
      301 gagcaccggg ccacggtgtt cccggatgag ctgttcgcgg atctgttcgc ctcgggtcgg
      361 ggccggccga gcgtgccggg tccggtgatc gcttcggtgc tggtgctgca ggcgctgcag
      421 gggctgtcgg atcgcgacgc ggccgaggcg ttgcgctgtg atctgcggtg gaaggtggcg
      481 tgcggcctgc cactagacga tgaggggctg cacgcgacga cgttgacgta ctggcgggcg
      541 aggctgcgcg cgtcggatgc gccgcaacgc atcttcgacg cggtccgtgg ggtgatcaac
      601 gccacgggtg tgctcaaggg tcgcgataag cgggcgttgg actcagtcgt gctggacgac
      661 gcggtcgcca cccaggacac ggtgacccag ctgatcgccg cgatccgccg ggtacgccgc
      721 gacgtgcccg gcgccgccgg gttcatcgcc gaacactgcc acgcccatga ctgggacgac
      781 ctggccaagc cgcgcatcga ctgggacgac accgccgccc gggacgcgtt gatcaccgcg
      841 ctggtcaccg acgcgttggg gatcctggac acgttcgccg acgccaacct cgccggtgat
      901 gagcgggccg gacaggcgct ggcgctgttg gcgctgatcg ccgggcagga cgtcgagcca
      961 gccgacgatt ctgacggcag cgacggccgg tggcgcatcg cccggcgggt ggcgccggat
     1021 cgggtcatct ccaccgtgga caccgagacc cggcacgtgc acaagacggt gcaccagcgt
     1081 caggacgggt tcaagggcca tgtcgcggtc gaacccgaaa ccggcatctt cacccacgtg
     1141 cagttggcca aggccaccgg ggacgacaac cacgaagcgg tcatcgggct gacgctgatc
     1201 gccagcgaac ccgccgggac ggaggttctg ggcgactcgg cctacggcac cggcgacatg
     1261 cgcgccgccc tggccgaggc cgggcaggtc gcggtgatca aaccgatgcc gctgcgagcc
     1321 gccgtgccgg gcgggttcac catcgacgac ttcgccgtgg acgaggacaa ccagcaggtc
     1381 acctgcccgg gcggacacac gcgccacatc accccgaacc gccacgtcgt cttcggcgtg
     1441 gtctgcgcca gctgcccgct gcgcgaacga tgcaccacgt ccaaggacgg ccgcaccctg
     1501 aggctgcacc cgcacgacgc tctcctccgc gccgcgcgac gatcctgggc caccgacgaa
     1561 gcgctgcgcg aggcctaccg tcagcaccga cccatggtcg aacgatccat cgcctggctc
     1621 atcggcaccc gcagccggtg ccggcgcctg ccctaccgcg gcgtcaccgc caacaactgg
     1681 tggctacaca cccgcgccgc cgcgctcaac ctgcgccgcc tgctcaacct cggcctcgcc
     1741 gccgacaacg gcgcctagac cctgcgcacc ccctgacacc gaggaccggc acgagtagcc
     1801 tgaccccgac ggggagggcg agctcgttgt ccagcagcaa tgctgtgacc taccgccgca
     1861 agacggccac tgagaggccc tccccgcccc aagactcgac ccccccgacc accatccggc
     1921 caacgggata gttcagcagg gtcctagtga cgggtgccgg tgccgcacgg gcatcgtcga
     1981 cacccgcacc ggtacccgca tcatgacgat ggaggacttc gcgat
//
LOCUS       DASRJA010000264        38010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1029262,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000264 DASRJA010000000
VERSION     DASRJA010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 38010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38010
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029262"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VFC01_11670"
     CDS             complement(<1..1333)
                     /locus_tag="VFC01_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80330.1"
                     /translation="MDAVLGLSVTPSAVGLVLVEGQDADGATMDRGAIDVGFRGRSSA
                     VQTCAQAAAAVRRSEAIAATRGHRLHSIGVTWSDDANAEASLLLKSLTDSGFDNVVPV
                     RLPEATEALARGIAEVIGYSTTAVCVLEPETVIALIVHLRDGAVQTSVNHRIVSEKDL
                     IRWLSTVFTRADWQPEALVMVGSGGDDELMPILEEALSVPVFAPAEAQLALARGAALA
                     SAQNVDITFTDHSHDYSHDHSHDYGERRPVAQRRRQLGQTGPLALLVAGVLTFTVSAS
                     IAVSLQLAPKKDNATSEPRPAAKISPETPAAVTHLQAPVALPSPVVEAVPPPPPESPP
                     VEPAPPPVYSEAPVSIPESPAAVTDAPPVEGTPPAVVAAPLPPEGAPLPPEQLAPPVA
                     PQPAERPGIMSRIRDRIRGGREDEVQPAPELAPPPPPAPEAAPPAPAAEPAP"
     gene            1728..1898
                     /locus_tag="VFC01_11675"
     CDS             1728..1898
                     /locus_tag="VFC01_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80331.1"
                     /translation="MAVHDYGFGHSMAACSCGWAGRRRYLKAAAQQDAWLHSMHEKCA
                     IAFPLMIPFSAA"
     gene            complement(1900..2847)
                     /locus_tag="VFC01_11680"
     CDS             complement(1900..2847)
                     /locus_tag="VFC01_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HZN80332.1"
                     /translation="MQLVSIDDIRAAAELIHPYVLRTPLVQASWADDDRPLWIKPENL
                     QSIGAFKVRGAFNAIGHLDESVRTRGVVAYSSGNHAQAVAHAAAVYGVPAHIVMPEET
                     PNIKVAATRTLGAEVVLCGPGQREKVAAEVVEKTGGVLVPPFDHPDIIAGQGTIGLEI
                     AEDLPTVENVLIPVSGGGLASGIGTAIKALCPHARVFGVEPELAADTAEGLRFGRRVD
                     WSIEDRNRTIADGLRSQPSALTFAHLQKVLDDLITVSENEIRLAVRELAYRAHLVAEP
                     SGAVALAAYRKGTTPPGRTVMILSGGNIEPPLLQEILAS"
     gene            complement(2875..3756)
                     /locus_tag="VFC01_11685"
     CDS             complement(2875..3756)
                     /locus_tag="VFC01_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80333.1"
                     /translation="MTGSEQWQAPSPTGDGREARQQPEDTGDQKVADWQRQLRWVAWL
                     TFLGLAVYYSQSVYFSAPEWLALAAAIGISIWCLAKPLGGPKYELREPSHLLGAFVSR
                     TSWGLVLFGALLTLGGVAGSMAAIYDVSTGRATVGDVFTDIGIFIEGWIAEMISPAYD
                     AELEKTHAYALFLLLIPGLLLLFFNLVPFCNRGKQFQVHPDGSVSVRQGDSWEPLMEY
                     QYSSVTADGTTIKFTPPPDGPPAVVLPQDRVFSREYEARLSVKVSAEFFRRLLAGRGF
                     DVESASSRSDSFTARRK"
     gene            complement(3804..4988)
                     /locus_tag="VFC01_11690"
     CDS             complement(3804..4988)
                     /locus_tag="VFC01_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN80334.1"
                     /translation="MPDLDVEALRALTPGCANRNHLNNAGAALMTTATIAAMTEYLQR
                     EALIGGYEAEAEAAEQIGDVYESLAELVGAHPSQIALFDNSTHAWNAAGYSVPLEPGD
                     RILTGRNEYGSAVLAYLQLAQRTGADVVVVPNDDSGQIDVAALADLIDERTKLIGLTW
                     VPTAGGLVNPAAEVGRLARAADVLYLLDATQAVGQFPIDVESIGCDMLTGTGRKFLRG
                     PRGTGFLYVGPRALDRLEPFVAEIRSATWAGGRSFTWVDGARRFETWENSYLNIVGLG
                     AAVRQALDIGLDRIGRRSAALGARLRTGLAEIDGVRVHDLGREQCAIVTTRVAGVDAG
                     DVANALSRRGINVSTTVAEHNQFDDRDVHPMVRLSPHYYNTEAEIDQAVEAVSALAAN
                     HS"
     gene            5013..5429
                     /locus_tag="VFC01_11695"
     CDS             5013..5429
                     /locus_tag="VFC01_11695"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZN80335.1"
                     /translation="MSGFSLPVVPRYAEVDQQGVVFNGHYLTWFDEACTGFLDHLGVA
                     YPDLIAAGHDIQVVHSEIDYLAGVRWRDSVRVAVRCERIGTTSFTLGFTILRRTADTE
                     EQTAIRGQNVYVVVSTDDWTKRQIPDPLREALTAYD"
     gene            5431..6105
                     /locus_tag="VFC01_11700"
     CDS             5431..6105
                     /locus_tag="VFC01_11700"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN80336.1"
                     /translation="MTENRVRAGYDAVAPTYAEQFRDELDHKPLERGLLAAFCDLSPP
                     GVIADIGCGPGHITRFLAARRDNVIGLDLSPAMIMVARQRNSGPAFAVGSMLHLPYPD
                     AAVAGVVAMYSIIHLSIEERAVAFAEFARILGPQGVLLVSFHVDGPGLAPGDVNHLTT
                     FLGHPVDMDGYFLNPTMITRELFANGFRVEARLDREPIADIEFSSRRCYILARRAVRS
                     SHGPRA"
     gene            6089..7120
                     /gene="speB"
                     /locus_tag="VFC01_11705"
     CDS             6089..7120
                     /gene="speB"
                     /locus_tag="VFC01_11705"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HZN80337.1"
                     /translation="MGHEHDHQRDLPPGMAEQLDLAHAGMVSFGHRPFLTEAEQLESW
                     RPDVAIVGAPFDISTTNRPGARFGPRAIRATAYEPGTYHLDLGLEIFDWLEVVDFGDA
                     YCPHGQTEVSHTNIRERVHAVASRGIVPVILGGDHSITWPAATAVADVHGYGNVGIVH
                     FDAHADTADEIEGNLASHGTPMRRLIESGAVPGTHFVQVGLRGYWPPQDTFEWMLEQG
                     MTWHTMQEIWDRGFKDVMRDAVSEALAKADKLYVSVDIDVLDPAHAPGTGTPEPGGIT
                     SADLLRLVRQLCYEHDVAGVDVVEVAPAYDHAELTVNAAHRVVFEALAGMAARRRDAA
                     QAKPGPPAR"
     gene            7337..7612
                     /locus_tag="VFC01_11710"
     CDS             7337..7612
                     /locus_tag="VFC01_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HZN80338.1"
                     /translation="MKYLVQWTASSPEATRATAKHFLETGGKPPDGVTQLGRWFGMNG
                     TGCAVVEATDPKGVFTLISEWQEFIQITATPALEDDEAGEVIAKLYG"
     gene            7643..8626
                     /locus_tag="VFC01_11715"
     CDS             7643..8626
                     /locus_tag="VFC01_11715"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZN80339.1"
                     /translation="MDLLDRLRLDVPVAQAGMGGGLAGPELAAAVAAAGGLGTLGLLP
                     PEELREAIGQLRAAAPDRAVAVNLLMPFVRRSHVEVCVQSRIHVAVIAFGMDRALLRS
                     LCEHGVFAFVMVGTEAQARRALECGASGLIAQGCEAGGHIAGDTPALTFLPRALEVAC
                     GRPVLLAGGIAVADDTRAALAAGAAGVVAGTRFLLTDEARAHPGYQHRILEADRTFRT
                     MLFGLGWPAPHRVVANAATRRWCHADGRSKLVPRLINAGSGVLAKLPDRAITPVLEKQ
                     TPGLPVFSPIAPTIGMPDSAVDRMALYAGETVLRLTSVISARQAVAELTPG"
     gene            8638..9015
                     /locus_tag="VFC01_11720"
     CDS             8638..9015
                     /locus_tag="VFC01_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HZN80340.1"
                     /translation="MTGVLFFDGACGMCTRSVYFLVRHDRTGHVQTEPLQGARVAERL
                     GIREHQLLEAMRWLDSSGAVYTGAEAWAAAWSVALGNRLPLLIYRVPGIRIIQNAVYR
                     WVASHRYRFPGTTPYCESHPVAC"
     gene            9032..9541
                     /locus_tag="VFC01_11725"
     CDS             9032..9541
                     /locus_tag="VFC01_11725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN80341.1"
                     /translation="MRQSDIVIRRTVAGDLEDIDRIYAHHVQTGVATFELTAPDAAEW
                     HRRLDAVIAAGLPFLTATRYGRVAGYAYCAPWKTRPAYRHTVEDSVYVAPDAVGCGIG
                     GRLLDALLNDCTRAGVREVIAVIVDADGAASLALHRNRGFVDAGRLTAVGFKHGRWLD
                     TVLLQRSLS"
     gene            complement(9548..10093)
                     /locus_tag="VFC01_11730"
     CDS             complement(9548..10093)
                     /locus_tag="VFC01_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80342.1"
                     /translation="MNSRLHPSQSAVLVLTALAALLAVAAGLLWLSGRGDDEMRVQGE
                     PMTDAQAAAQVLGSAKQIVSTAELREVTGGYAFVSCKNANEPPYQVTVYMNFRLPQGN
                     SVKYLRDVAAALATHGWTTAPAMGEHFGQKLTRDGVTAVFYRIGNDTDFATMRLYGEC
                     RNVADHRNDNPAWREVTDQLS"
     gene            10165..10971
                     /locus_tag="VFC01_11735"
     CDS             10165..10971
                     /locus_tag="VFC01_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80343.1"
                     /translation="MDTVIVQAAMAEAAATGTPVAELSLDRIARRAGVSRSTVFRRVR
                     SRRALDDAVREAGGDPGTRIGVRERAIAAATELIVADGVAAMTVEAVTRRVGCAATSI
                     YAAFGDREGLLAAVFDRHAPLPVVEELLAAHPRPFADFESGVRAIYTAVFDSVVDDTA
                     VLQALFAEILAKPNGIGTQFFRDRILPRITSTVGAWLNEQIHAGRCADLPLSLLIPLL
                     ISPISVHLLTRPRLINAGQPVPDRHAVIEAMTSAFCGATATTAHRPASTS"
     gene            10968..11819
                     /locus_tag="VFC01_11740"
     CDS             10968..11819
                     /locus_tag="VFC01_11740"
                     /inference="COORDINATES: protein motif:HMM:NF020227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80344.1"
                     /translation="MKSVVVTGSSSGIGRACALMLDRNGFRVFAGVRKDADGDALRSE
                     ASGSLVPVHIDVTDAASIHSMADQVKAEVGEAGLHGLVNNAGTTLPCPIEYLPLDGFR
                     RQLEVNLVGPLAVTQALLPMLQTARGRVVNVTSGAGKAGVPLMAPYVSAKHGLEGLSD
                     VMRLEFRQLGIQVAVIEPGFVGTSMRGKLQRDTEAALTSLPDEGSRRYGPALEKLAAE
                     ISHHATTGSPPDVIAESVLHALTSKKPRTRYPSGAGARQMLFMRRILPDRRFDRLILR
                     TAGLTGF"
     gene            complement(11824..12297)
                     /gene="soxR"
                     /locus_tag="VFC01_11745"
     CDS             complement(11824..12297)
                     /gene="soxR"
                     /locus_tag="VFC01_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HZN80345.1"
                     /translation="MESHDLLTVSEVAERSGFAPSAIRFYDKQGLISASRTSGGQRRF
                     ERNMLRRLAFIRAARNVGLTLDEIAAALELLPDGRTPTRVDWTRLSKEWRQRLDEQIA
                     GLIALRDKLDSCIGCGCLSLKRCAISNPSDSASGAGPGAVYLPKSLRRQVVGRPA"
     gene            12353..13072
                     /locus_tag="VFC01_11750"
     CDS             12353..13072
                     /locus_tag="VFC01_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80346.1"
                     /translation="MTTSEQTSPVAIVTGGSRGFGRAAAVALLDRGWTVVADARRAGE
                     LEAMADEVDSPRLITIAGDVTDTDHRAALVDGATAAGPLCLLINNASRLGPSPQPRLA
                     DYLAADLRAVYDTNVFAPLALFQAALPALTDNRGVVVNVTSDAAVEPYEGWGGYGSSK
                     AALDHLSAILAAENPDVGVYAFDPGDMRTEMHQSAFPGEDISDRPEPESVVPALLRLL
                     DTRPAPGRYRAQDLLAIGADA"
     gene            13069..14133
                     /locus_tag="VFC01_11755"
     CDS             13069..14133
                     /locus_tag="VFC01_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HZN80347.1"
                     /translation="MILKPQTQFERSPASDAVEPPEARGMPRDGVKLMVATPDDVVHT
                     RFADLGQHLVPGDLLVVNNSATLPAAVDARRPKRGPIAVHFSAALDDHLWVVELRPAT
                     NATAHLPDVEPGERIGLPDGAALVVIASYPQPGVAGSRLWSARLAIEGGVTTYLCRHG
                     RPIRYSYIPREWPLSSYQTVFAHRPGSAEMASAARPFSAELVTSLVSAGIVIAPITLH
                     AGVSSAEEGEPPSAERFEMPTSTARLINLTRASGGRVIAVGTTATRAVESAADTFGTV
                     RPATGWTDLVLGPDRPPRVVDGLITGWHDAGASHLLLLEAVAGPALVAAAYREALEFG
                     YLWHEFGDSALLLPISPRSR"
     gene            complement(14111..14743)
                     /locus_tag="VFC01_11760"
     CDS             complement(14111..14743)
                     /locus_tag="VFC01_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80348.1"
                     /translation="MKVNDGVTAESPARRTQAERTAATRALLIDAARKLFADNGFTEV
                     STQAIVAAAGVTRGALYHQFDDKAALFAAVYEEVERALVADIVRQIADEQPPDPLEAM
                     RLGASLFLDRCSAPDVQRIVLIDAPAVLGWDRWRAVGVKYGLGVIEAMLAHAIAEGAI
                     PEQPLRPTAHVLLGALDEAALFVSRATNPDQARQEMDAVCDRLISGIAGK"
     gene            14804..15643
                     /locus_tag="VFC01_11765"
     CDS             14804..15643
                     /locus_tag="VFC01_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN80349.1"
                     /translation="MPQVVLDQATIDYRVFGPEDSPHPPVLFVHGILVDSRLWDRVAE
                     GLARQGFRCYLPNWPLGSHTIPVNDPAALSPRGVATLIRDFIVALGLSDVTLVGNDTG
                     GGLCQLVIDAYPEHIGRLVLTNCDAFDKFPPFPFTLVFALLRGPISIKVLFEQMRIKA
                     LRHSPLGFGLLVHPEPQLTASWLEPGRTDARIRRNLATLLRNVAKTDLTDVATRLRQF
                     SKPVTIVWGQRDRAFTPALGRRLAAAFPNATLIEVPGARTFVSLDAPAAVIDAIAKVG
                     ATA"
     gene            complement(15640..16524)
                     /locus_tag="VFC01_11770"
     CDS             complement(15640..16524)
                     /locus_tag="VFC01_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HZN80350.1"
                     /translation="MPETTSDTQNLKPHFEDVQAHYDLSDDFFRLFLDPTQTYSCAYF
                     ERDDMTLEEAQIAKIDLSLGKLGLQPGMTLLDIGCGWGATLNRALERYDVNVIGLTLS
                     ENQKAHVEQVFAASDSPRSKKVLLQGWEQFDEPVDRIVSIGAFEHFGRDRYDDFFKKA
                     YAILPQDGVMMLHTIIKPTDEEFAETGLPLTMKLLKFSKFIMDEIFPGGDLPKPTTVE
                     EHAAKVGFELKRAQRLRLHYARTLEIWAAALEAHKDEAIAIQSEEVYDRYMKYLTGCA
                     ELFRDGYTDVCQFTLVKS"
     gene            16691..17605
                     /locus_tag="VFC01_11775"
     CDS             16691..17605
                     /locus_tag="VFC01_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HZN80351.1"
                     /translation="MSSVVDDVRGFNRFYTRMLGLLRPHLAGSAFGLTEARVLFELAH
                     RDDAAVSDLRRELDLDAGYLSRILSSFTSSGLVVREKSAADGRRQVVRLTDDGRRAFD
                     ELDRLQADAIATMLTPLDDGQQAELVTSMGRIRRALGDEPRSKGLVLRSPAPGDLGWV
                     VERHGYRYSAEYGWDTDFEALVARVVADFAERDDTKREAAWIAELDGERVGCVFCTAA
                     DVKDTAQLRLLLVEPSARGAGVGTRLVDECLRFARRSGYRRITLWTNDVLLAARRIYQ
                     RAGFHCDRSEPHHSFGRGLVGEYWSRDL"
     gene            complement(17610..18584)
                     /locus_tag="VFC01_11780"
     CDS             complement(17610..18584)
                     /locus_tag="VFC01_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HZN80352.1"
                     /translation="MNVSHQAPRKWVRRMAAAMAAAALLPGLVGVIGGTPTAGAAVEV
                     LSVPSPSMGRDITVQFQGGGPKAVYLLDGLRARDDRNGWDIETGAFGWFDGSGVSVVA
                     PVGGMSSFYTDWYRPAVGNGTTQTYKWETFLTSELPAWLAANKGVSPTGNAVVGLSMS
                     GSSALILAAYHPGQFVYAGSLSGFLNLSRGLWPVLVGISMGDAGGFNALDMWGPYGGP
                     AWQRNDPMLQIGRLVSNGTRIWVYSGNGNPTDLDAGLGNAQIPAQFLEGLTIRTNKDF
                     QAAYTRAGGSNGVFNFPPNGTHSWGYWGGQLQAMKPDIQRVLGAVPAA"
     gene            18705..18929
                     /locus_tag="VFC01_11785"
     CDS             18705..18929
                     /locus_tag="VFC01_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HZN80353.1"
                     /translation="MKAKVGDFLVVKGTTVEQHDQRGVITEVRSADGSPPYVVRWLST
                     DHVATVYPGPDALVVTPAEQEAADARHRAH"
     gene            18954..19157
                     /locus_tag="VFC01_11790"
     CDS             18954..19157
                     /locus_tag="VFC01_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80354.1"
                     /translation="MTKPSGEFGDDAPEADVIEQLRSTDVSEEDTWRDAARVASARDW
                     EANEADLIEQAIAVPDDDSEFDR"
     gene            complement(19160..19357)
                     /locus_tag="VFC01_11795"
     CDS             complement(19160..19357)
                     /locus_tag="VFC01_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80355.1"
                     /translation="MAVTAEPTTLPALLKELGVQRASVEKQKPALRAWLATHNASHPL
                     RVSLCCNGYGLLLKESSALHN"
     gene            19507..20088
                     /locus_tag="VFC01_11800"
     CDS             19507..20088
                     /locus_tag="VFC01_11800"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HZN80356.1"
                     /translation="MRLDGYLRRCAALVGALTTLAVMPTTAPARGDDANPLLDLVDAA
                     AQRLQTAEPVSATKWINGGSIDDPPRVEQVLAAVSADATGRGIDPDYVRQMFTDQIDA
                     TEAIEYTRFGQWKLDPGSAPRIAPDLAASRATIDGLNTEMVEQVALRWSVLHSPDCTR
                     MLDDATTAVASARMLDPLYQQALAFSSQSYCAP"
     gene            complement(20093..20380)
                     /locus_tag="VFC01_11805"
     CDS             complement(20093..20380)
                     /locus_tag="VFC01_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HZN80357.1"
                     /translation="MVIVAGQIVVDPQQRDDYLSGCVEVVRQARGTAGCLDFALSADL
                     LEPGRVNIFERWESQAAVEAFRGSGPSDDQGAAIVAASVAEYDIAAERTLM"
     gene            20473..20793
                     /locus_tag="VFC01_11810"
     CDS             20473..20793
                     /locus_tag="VFC01_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018162967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80358.1"
                     /translation="MNLREARQVIGSLESDPDAARVFGEAYQTTDGATVIPVTTVRGA
                     SPKPAGVFVVKDGKASWVPAVDAGRIAMFGILVGLVSATLAGVAMVRRPPWPDLHGDI
                     SSRR"
     gene            complement(20735..21619)
                     /locus_tag="VFC01_11815"
     CDS             complement(20735..21619)
                     /locus_tag="VFC01_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HZN80359.1"
                     /translation="MDQDLVGLAFGAGLVAALNPCGFAMLPAYLALAVRGEDLGRRAA
                     IGRAVTATTAMAAGFLVVFGGFGVLTVSAAATVQRYLPYVTVLIGIVLVALGVWLLAG
                     REIRVLGGMKAGARWAPTARLGSMFGYGISYAIASLSCTVGPFLAVTAAGLRGGSVLG
                     AMAVYVAYAGGFTLVVGVLAMAAALASSATVDRMRRIVPYVNRISGVLLVIVGLYVGY
                     YGLYEIRLFTANGNPEDPVIAAAGRVQGAIAGWIHQHGAWPWLVALAVLIVFAAAWRA
                     SAGSRYRRADRATADDEP"
     gene            complement(21632..22135)
                     /locus_tag="VFC01_11820"
     CDS             complement(21632..22135)
                     /locus_tag="VFC01_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HZN80360.1"
                     /translation="MSTRFSRLIRSLVLSAAAAALAFGLVSPPAAVADDRLQFTGTTL
                     SGAPFSGASLIGKPAVLWFWTPWCPFCNAEGPSVSQVAAANPKVTFVGVAARSDVAAM
                     QAFVSKYNLTFTNLNDADGSIWARYNVPWQPAYVFYRADGSSTFVNNPTAAMPEQELS
                     DRVAALS"
     gene            22239..22751
                     /locus_tag="VFC01_11825"
     CDS             22239..22751
                     /locus_tag="VFC01_11825"
                     /inference="COORDINATES: protein motif:HMM:NF042492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6653 family protein"
                     /protein_id="HZN80361.1"
                     /translation="MSLEAWLANVFGLKGQDAWRRHANPWSVYTRIPIPAVLVAAIRS
                     RTWIGWWSLLPIAVCLIWTVVNPRAFPPPRTLDSWASRSVMGETLWAERKSTPLPSRH
                     RTAPVVLGALSAAGLPFLTWGLVVLDPWLTLFGLAIQMLGKLWFLDRMALLYNDVSSR
                     AAPIPPRTDR"
     gene            complement(22708..23589)
                     /locus_tag="VFC01_11830"
     CDS             complement(22708..23589)
                     /locus_tag="VFC01_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03854 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HZN80362.1"
                     /translation="MKIRFGVGLGADTGPEQLARIVDHLEATGVDSLWFSELVYSKAV
                     DPFVGMAYALARTTNLKVGTSVAVLPGRHPVLVAKQLASLAGLAPKRVLPVFGLRSAI
                     PAEREIFVVPDGERAAVFDESLRLLRSVLEQQDVAFAGDYFAVSSAVIEPRPVKPLDI
                     WLGGSAPAAFRRIGQLGDGWLGSFLTPDEARAGREQIQRAATDAGREIEPDHFGINLA
                     VSDGDVPDALIAAVRRRRPDVEPGDLIASGWSQLHRQVDAYLAAGLTKFVIRPALPAN
                     IDDFIDRFVAELVQREN"
     gene            complement(23729..24310)
                     /locus_tag="VFC01_11835"
     CDS             complement(23729..24310)
                     /locus_tag="VFC01_11835"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN80363.1"
                     /translation="MVNIATTSDGQRASGARLTRYRKRQQRLRQRLQAKKTNSARRLL
                     KKRRRKEARFATDLNHQVSKRIVAEAQRTGRGISVEKLAGIRARVRLRKPQRATLHSW
                     AFAQLGGFVAYKANRAGVPFVEVDPAYTSQTCHQCGWVDKRNRRSQSEFECRRCGFVG
                     HADHNAAIVIAHRGVERWGEVMRPYAAPTLAAS"
     gene            complement(24256..24909)
                     /locus_tag="VFC01_11840"
     CDS             complement(24256..24909)
                     /locus_tag="VFC01_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN80364.1"
                     /translation="MMQVVKVRMLPTPEQAAALQATLRTCNSAASWLSAAMHATHVHR
                     KADAQQQFYGELKQRFGLSAQPAIRVIGKVVDAYRTLRASIDAGNFGPPSADKRKAVQ
                     DTPVRFRTDAAQPFDARCLSWQIPETAGGRTATVSIWTTRGRCKGVRVLAATRDLAML
                     RTHPIGETDLIYRDGNWYLYAAVEVPQAPLTDPVNGFIGGSSTSPPPATGNVPAARG"
     gene            25137..25550
                     /locus_tag="VFC01_11845"
     CDS             25137..25550
                     /locus_tag="VFC01_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6188 family protein"
                     /protein_id="HZN80365.1"
                     /translation="MNQPWVRGCVVQRIMFRRGLVLNLDDNELVISVPFHLTLPATVR
                     DDEEAVLIDPKAARAEERALFDFAGSTCTHASWDEDGSLHLRFSDGHEIHAHSDADGT
                     AWELYGKNHGYAACLPRGRVRVVRHDLPEDESAER"
     gene            complement(25547..26317)
                     /locus_tag="VFC01_11850"
     CDS             complement(25547..26317)
                     /locus_tag="VFC01_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007300139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN80366.1"
                     /translation="MPDWSGRDYADVSSPQRAMATEAVTALRFGAADRVLDVGCGDGY
                     LTRAVAGMVPDGCAVGVDASPRMIETAHRGGEPTGSGPWFVVADARQLPFAEFFDAVV
                     SFNALHWVREQRQALREIATVLRPGGRALIQVVCGGPRTSIEATTMAVCESPRWARWF
                     DGFSAPYTHVDPEAYGELAASAGLTLDKITVTDREWDFGSRDALSRWCAVGSTAWTDR
                     LPAQNRAAFVADQVQAYEPVAGRQGLFRFTQMRALLHR"
     gene            26380..27570
                     /locus_tag="VFC01_11855"
     CDS             26380..27570
                     /locus_tag="VFC01_11855"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN80367.1"
                     /translation="MRARIMALAVTAVVLVAASGCADESKPQSSTTSPPATSETAKPM
                     NDALAQRLDAAVNQAMTAAGIPGAIVGIWGPDGDYVRAFGVADKTTRAPMKADFYSRI
                     GSVTKTFTVTAVLQLADQGKLRLDDPIAEFVDGVPLGDKITLRQLARMQSGLFNYSAS
                     PEFQQAMFADPRHPFTPRELLNYALAQPNQFPPGEGFEYCNTNTILLGLVVQKVSGQP
                     LNAYIEDQILAPLGMSHTSFPTTNAFPEPHAQGYTVQTADGKETTATDWDPSWGWAAG
                     AMISTLDDMRIWAPALASGKLLTPQMQEQRLQTVGQPGMPPQDGYGLGIFNLGGWIGH
                     NGSLPGYQTVVVYLPQKQTTLVIMINTDIEYQGNEPSTTVATAITKELSPDHIYSFGP
                     QMPR"
     gene            27590..28054
                     /locus_tag="VFC01_11860"
     CDS             27590..28054
                     /locus_tag="VFC01_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN80368.1"
                     /translation="MQGSAKVTMSAPADQIWNLIADVRNTGRFSPEVLEAEWLDGATE
                     PALGARFRGHVKRNEIGPVYWTTCRVTACEPGREFGFDVLVGDRAANTWHYRLTPVDG
                     GTEVTESFRMPDNLFSKLFGVLGGQLRQRRNVRDMRTTLERIKAVVEAPEGA"
     gene            complement(28064..28498)
                     /locus_tag="VFC01_11865"
     CDS             complement(28064..28498)
                     /locus_tag="VFC01_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80369.1"
                     /translation="MRLSKFVSAAMIVGALGLGAIGPGGALASADPVVPAPLKPHDNF
                     CPPWCGNGNGNGNGNGPDFKHWDGDKGPWWASNRHEWWDDRNGPPPWGWGPPPAYQWD
                     GGPPRPFNYWGYDVNPVWDDGFHQWGIWLFGLWIPVFGIGFQ"
     gene            complement(28669..29631)
                     /locus_tag="VFC01_11870"
     CDS             complement(28669..29631)
                     /locus_tag="VFC01_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN80370.1"
                     /translation="MALGIDAQVLAELAPLMEAVGESEPLAIGDVEGRRIDGHRMFDY
                     VASTWQPVPDVEVGRRTLTTADGTTLALGWYHMPGRRHPGSAALYLHGGGMILGLEHL
                     GLVYDLAVREYVATSGVPMLVVDYRIAPEHPHPIPVEDCYAALRWLADNAATLGVDPG
                     RIGVMGDSAGGGLAAGVCLLARDRGGPAIAQQLLIYPMLDDRAQTPDPQLLPYLTWTY
                     DDNVTGWAALVGESAGADGVSPYAAPARATDLTGLPDTYIDVGDLDIFRDEDIMYARH
                     LSDAGVPTELHLHPGCPHAFEALARRADVSRRAIADRIRRLRTL"
     gene            29657..31114
                     /locus_tag="VFC01_11875"
     CDS             29657..31114
                     /locus_tag="VFC01_11875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN80371.1"
                     /translation="MTEAQTDTTTGQRISPFAAGAVLPIAAVTTVLHCIATTFGDSYW
                     FDEVYMLAIGRYHLDWGSADQPPLAPALAALTDAVAPDSLIALRAPAVLATAGGIVVA
                     GLIARELGCDRRAQGFTAAAQATGVWTTLFGHWLAPYTLEPVQWLLAIWLLVRWVRLR
                     DDRLLLALGVVVGVAALTKFQILLLCAVLVIAVAATGPRDLMRRPMLWAGAGIAALLA
                     APTLVWQQLHGWPQLRMAPVVAGEAEALYGGRPGIAVQLIAFAGVAGAALMLYGLWRL
                     FRDDELRDYRFIGVAFVVLYVVFVVTAGRPYYLAGLYAPLAAPGALGLQRRREIGPSR
                     LRWLIWPGYVLSVALAVGLLILSVSIVRSDVGEQIAQRTADAYRALPRVQRDRAVVFG
                     ESYIVAAYLDGYSTRYQLPEAYSANRSYGYFPPPPSDHDTLLYIGRDPGALRSYFADV
                     RRVGDVGEELHAYLLTGQRQSWVEIWPRLRTLTVS"
     gene            31187..31690
                     /locus_tag="VFC01_11880"
     CDS             31187..31690
                     /locus_tag="VFC01_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN80372.1"
                     /translation="MGQWSREELEDAFQAYQQDVIEVGKTWDWSSYADHFTEDAKYVE
                     HALGNMEGRENIREWIVSTMNMFPGSEMPVYPVEWYSIDTDKGWVIFKNINTMKDPGD
                     GSVHGAAVITVLEYAGDGKWSYEEDAYNPMNFLVMIQGYVKRCHELGTLSDDARVFAK
                     NMNWELS"
     gene            complement(31687..33600)
                     /locus_tag="VFC01_11885"
     CDS             complement(31687..33600)
                     /locus_tag="VFC01_11885"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN80373.1"
                     /translation="MRNRHAGQPFTSSDVQIAEALLDVSIPTLMLSLVHMSGDPSLIR
                     GELKPAGLFLNEVQGYMSEEDKAAVRKIALEVIADYRDRGCPEPEPIGAELLQEMMQW
                     LVCEPVPPEYVPMLLEEMELDGQDARAVPATPADFPVVIIGCGESGLLAGIRLKEAGI
                     PFTIVEKNAGVGGTWWQNTYPGARVDVGNHFYCYSFEPTDHWTHFFAEQPELQAYFQT
                     VMDKYDIGRHVLWETEVIEASWDDDTATWSVQARDRNGAITTLTARAVISAVGQLNRP
                     HLPDIDGHKDFAGPAFHSSEWDHSVDLRGKRVAMIGAGASGFQIAPAIAGDVEQLTVF
                     QRTAQWMFPNPNYHAEVGPGVQWALRHLPFYGRWYRFLLFWPGCDKGLAAAYVDPDYP
                     DQQKAVSEINEITRIMFTEWITSQVGDDAELLARVVPDYPATGKRTLQDNGSWLRALT
                     RDNVELVRTGVDHIESDAVVTEDGERYPADIIVYATGFQANKMLWPMTIRGRDGAVLS
                     ERWGERPSAYLGITVPGYPNFFCMYGPGTNLAHGGSLIFHSECQMRYITECLELLIRG
                     RHRSLEPREDKAADWHERSQAEMRRLVWSQPSVKHSFYKNSFGEVHILSPWRLVDYWA
                     WTREPDPDDFVIR"
     gene            33652..34971
                     /locus_tag="VFC01_11890"
     CDS             33652..34971
                     /locus_tag="VFC01_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN80374.1"
                     /translation="MTAAGSAPTLVKVGIAARANGTAPPDVPLTDIDLGDWSFWVQDD
                     DLRDGAFATLRREAPVSFHPALETPGVQEGPGHWAVTRYDDVWFASRHPHIFSSHPSI
                     TINDANPDVSEYFGSMIVMDDPKHLRLRNIVSRAFTPKVVARTETSVRDRARRLVESM
                     IEAHPDGSGEVVAELAGPLPLQVICDMMGIPEEDHQRMFHWTNVILGFGDKDIATDYE
                     EFLKVSMDIGAYATALAEDRRANPRDDLTTALVQAEVNGERLSSAEVASFFILLVVAG
                     NETTRNAISHGVLALTRFPDERAKWWADYEAMAPTAVEEIVRWASPVIYMRRRLTEDC
                     ELSGVKMAAGDKATLWYCSANRDEAKFADPWTFDVARTPNPHAGFGGGGAHFCLGANL
                     ARREISVVFEELRRQMPDIQATEEPSRLLSPFIHGIKRLPVSWTPPR"
     gene            complement(34989..35678)
                     /locus_tag="VFC01_11895"
     CDS             complement(34989..35678)
                     /locus_tag="VFC01_11895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZN80375.1"
                     /translation="MTAARPAVLFDVDGTLVDSNYLHVHAWYRAFRELGIDIEAWRIH
                     RSIGMDGSTLVATLAERADDDTRSRLKDLHSQYYREMAPLLRRLPGTRELLEAVDGLG
                     LQIVLATSAPEDELAILRNVLASDDLISVVTSSKDVDTAKPRPDIIQVALDRAGVDAA
                     NAVFIGDTIWDVEACERAGVPTIAVLSGGVSGGELEKAGAQAVFENPGDLCAHLDDTA
                     IAALAKAVQTV"
     gene            complement(35675..36115)
                     /locus_tag="VFC01_11900"
     CDS             complement(35675..36115)
                     /locus_tag="VFC01_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80376.1"
                     /translation="MSNTVIWIVVAVVVAIIIVALLAFLARNKRRHSQAEHIREEVGR
                     QTQRLDKREAFADETQAKARAAQAEAEAKAAEAARLQQRAASHREVVTTSREELDAQR
                     ERADALDPKRRDDQGPKHDDTRREGADEARQAQADTPTRKQHRL"
     gene            36264..36398
                     /locus_tag="VFC01_11905"
                     /pseudo
     CDS             36264..36398
                     /locus_tag="VFC01_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015381801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
     gene            complement(36622..37023)
                     /locus_tag="VFC01_11910"
     CDS             complement(36622..37023)
                     /locus_tag="VFC01_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZN80377.1"
                     /translation="MKRFGLFCAALCLVAAFATACGGSSNTTQETQTGTATATGTAEA
                     TSGPAAASAATITIANMSFGEAITVPPGATITIKNDDSAEHSVTSDTAGKFDVEVEGK
                     EQGTLTAPTEPGEYAFHCTYHPSMHGTLIVK"
     gene            complement(37127..>38010)
                     /locus_tag="VFC01_11915"
     CDS             complement(37127..>38010)
                     /locus_tag="VFC01_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN80378.1"
                     /translation="ERAHWFPAVNSGLVFGNAVTDTNGKAPASSDTTLLADTDGVLRL
                     NGYKFYSTGTLFADRIAVSAIDAEGRDLQAIVPTDRTGVELYDDWDGFGQRTTASGGT
                     RFTDVVVHPVEVTTVSDGKHLGHSTAFLQLYLAAVTAGIAHGVLDDAVAYVREKARPA
                     SHSLADTASGDPFILQAVGDIAANAAAAEAVVLTAADAIDAVVDNALQADPDALAELS
                     VTVAKGQLVAERLTLVAAERLFDTGGASATARKLNLDRHWRNARTVATHSPLAYKAYA
                     AGNYAVNGVWPPANGYF"
BASE COUNT         6367 a        12566 c        12452 g         6625 t
ORIGIN      
        1 gcggggcggg ctcagcggca ggcgccggcg gggcggcctc gggtgccggc ggcgggggcg
       61 gtgcgagctc aggagcgggt tgcacctcgt cctccctgcc gccacggatc cggtccctga
      121 tccggctcat gattcccggg cgttcggccg gctgaggggc gacgggcggg gccagctgtt
      181 ctggcggcaa cggggccccc tccggcggca gaggtgcggc gaccaccgcg ggtggggtgc
      241 cttccacagg cggcgcatcg gtgacggccg cggggctctc gggaatgctg accggcgcct
      301 cgctgtacac cggcggcggt gcaggctcga cgggcggcga ttctggcgga ggcggtggca
      361 ccgcctcgac gaccggcgac gggagcgcga ccggcgcctg gaggtgtgtg acggcggcgg
      421 gtgtctccgg agagatcttg gcggcgggcc gcggttcgga cgtggcgttg tccttcttcg
      481 gcgccagctg caggctgacc gcgatggacg ccgagactgt gaacgtaagc actcccgcca
      541 ccaacagggc caacggtccg gtctgcccaa gctgcctgcg gcgctgcgcg acggggcggc
      601 gctctccata gtcgtgggaa tggtcatggg aatagtcatg ggaatggtcg gtgaacgtga
      661 tgtcgacgtt ctgcgcggac gccagcgctg cgccccgcgc gagggcaagc tgggcttctg
      721 cgggcgcgaa gaccggcacc gacagcgcct cttcgaggat cggcatgagc tcgtcgtcgc
      781 cgcccgatcc gaccatgacc aatgcctcgg gttgccagtc ggcgcgggtg aacaccgtac
      841 tcaaccaccg aatcaagtct ttctcggaga cgatccggtg gttgaccgag gtttgcaccg
      901 cgccgtcgcg tagatgcacg atgagcgcga tcacggtctc cggctcgagc acgcacactg
      961 ccgtggtgct gtacccaatg acctctgcta tcccccgggc caacgcctcc gtggcctccg
     1021 gcagccgaac cggcaccacg ttgtcgaaac cggagtcggt caacgacttc agcagcagcg
     1081 aggcttcggc gttggcgtcg tcgctccagg tgacgccgat ggagtgcaga cggtggccgc
     1141 gggtggcggc gatcgcttca ctgcgccgaa ccgcagcggc ggcctgcgcg caggtctgca
     1201 ccgcgctgga ccggccgcgg aagccgacgt cgattgcgcc gcggtccatg gtggcgccgt
     1261 cggcgtcctg cccttctaca aggacgaggc cgacggcgga cggcgttacc gataagccga
     1321 gtacggcgtc cactcacacc ccttcggacg tccacctatc cgggtgaccc tacctgcccg
     1381 gggcgatcgc agctcaccgg gcgctggata gcgtaattcg ctgacggagg cgaaatatgc
     1441 tgtgccgcat gaaactcctg cgcatggtgc ggcagtaact ttgccgatga gttgctcatc
     1501 gctgctccgt gtcggcggtc gacccgcttg tcggcgtggc tgagtcgtaa tcccgtcgaa
     1561 atcgaaatcg cgccaaaccc aggtcagcat gggaaccaga tcgcacgcat gcgcgtctga
     1621 caggacaacg gggtcgggtc gacgtaggag gctgaccgat gtcgctagcc gcacggatag
     1681 acgcaataaa cgctggcgat tttccgcgac cagacgttct gggcgtcgtg gccgtacacg
     1741 actatgggtt cgggcattcg atggccgcct gctcgtgcgg ctgggctggc cgtcgccgat
     1801 acctgaaggc cgcggcgcag caagacgcgt ggctgcactc gatgcacgag aagtgcgcga
     1861 ttgcattccc gttgatgatt ccgttctcgg cggcctgagt cagctagcca aaatctcttg
     1921 cagcagcggc ggttcgatgt tgccgcccga caggatcatc actgtacggc ccggcggcgt
     1981 tgtgcccttt cggtaggcgg ccagcgcgac cgcaccgctc ggttcggcca ccagatgcgc
     2041 ccgatatgca agttctcgca cggctaagcg aatttcgttc tccgacaccg tgatcagatc
     2101 gtccaatacc ttttgaagat gcgcgaaggt cagtgcggac ggttgtgatc gcaagccatc
     2161 cgcgattgtc cggtttcggt cctcgatgga ccagtccact cgacgtccga agcgaaggcc
     2221 ctccgccgtg tctgccgcca gttccggttc gacgccgaag acgcgggcgt gcgggcatag
     2281 cgctttgatc gcggtgccga tgcctgatgc caatccgccg ccgctgactg ggatcagcac
     2341 gttttccacc gtcggcagat cttccgcgat ctcaaggccg atggtgccct gcccggcgat
     2401 gatgtcgggg tggtcgaacg gcggcacgag cacgccgccc gttttctcca ccacctcggc
     2461 ggcgactttc tcgcgctggc ccggcccgca cagcacgacc tccgctccga gtgttcgcgt
     2521 cgcggccacc tttatattcg gcgtctcctc cggcatcacg atgtgcgcgg gcacgccgta
     2581 aaccgccgcc gcatgtgcga ccgcttgtgc gtgattgccg ctcgaatagg cgacgacgcc
     2641 gcgggtccgc acggactcgt cgagatgtcc gatggcgttg aaggcgcccc gaaccttgaa
     2701 cgccccgatc gactgaaggt tctccggctt gatccacagc ggccgatcgt cgtcggccca
     2761 gcttgcctgg accaacggag tgcgcaggac gtaaggatgt atcagctccg cggcggcgcg
     2821 gatgtcgtcg atgcttacca gctgcacgag tccagtctgc ccgaccggcg ctggttactt
     2881 ccggcgtgca gtgaagctgt cgctcctcga cgacgccgat tcgacgtcga accctcggcc
     2941 ggcaagcagc cgcctgaaga actcggcgct gaccttgacg gacagtcgcg cctcatattc
     3001 acgggagaac acccgatctt gcggcaacac gacggccggt ggaccgtcgg gcggcggtgt
     3061 gaatttgatt gtcgtgccgt cggcggtgac ggacgaatac tggtactcca tcagcggttc
     3121 ccagctgtcg ccttgtcgca cggacaccga accgtcgggg tggacctgaa attgcttgcc
     3181 ccggttgcag aacgggacca ggttgaaaaa gagcaacagt agacctggaa tcagcaacag
     3241 aaacagcgca taggcgtgcg tcttttcgag ttcagcgtcg taagcgggag agatcatctc
     3301 ggcgatccag ccctcgatga agatcccgat gtccgtgaag acgtcaccga cggtcgctcg
     3361 tcccgtggag acgtcgtaga tcgcggccat ggagccggct actccgccga gtgtgaggag
     3421 cgcgccgaaa agcaccagcc cccaactggt tcgggatacg aacgcgccaa gcaggtggct
     3481 cggttccctc agctcgtact tcggtccgcc cagcggtttg gccaggcacc agatgctgat
     3541 gccgatcgcc gcggccagtg cgagccactc tggcgcggag aagtagacgc tctgcgagta
     3601 gtacacggcc agcccgagga aggtcagcca tgcgacccaa cgcaactggc gctgccagtc
     3661 cgccactttc tggtcgccgg tgtcctcggg ctgctgcctg gcctcgcggc cgtctccggt
     3721 cgggctgggc gcctgccatt gctcgctgcc tgtcacgcga agatcgtagc ggcaaacact
     3781 acagaagagg ggccgtcagc aaattagctg tggttggctg cgagggccga gacggcctcg
     3841 acggcctggt cgatctccgc ctccgtgttg tagtagtgcg gcgacagccg caccatcggg
     3901 tgcacatcgc ggtcatcgaa ttggttgtgc tcggcgaccg tcgtgctgac gttgatcccc
     3961 cgccgcgata gcgcattcgc gacgtcccca gcgtccacgc cggcgactcg tgtggtgacg
     4021 atcgcgcatt gctcgcggcc cagatcgtgc accctcacgc catcgatctc ggccaggcca
     4081 gtacgcagcc gagcaccgag agccgcgctg cgcctcccga tccggtcgag gccgatgtcg
     4141 agggcctggc gaaccgcggc accgaggcca acgatgttga ggtaactgtt ctcccaggtc
     4201 tcgaagcgcc gtgcgccgtc gacccatgtg aaggagcgcc cgccagccca agtggcggaa
     4261 cggatttcgg cgacgaacgg ttcgagccga tcgagcgccc gtggtccgac gtagaggaag
     4321 cccgtgccgc gggggccgcg caggaacttg cgccccgtgc cggtgagcat gtcgcagccg
     4381 atggactcga cgtcgattgg gaactggccg acggcctggg tggcgtcgag aaggtagagc
     4441 acatcggcgg cgcgcgcgag gcggccgacc tcggcagccg gattcaccag gccgccggcg
     4501 gttggtaccc aggtaagccc gatcagtttg gtccgctcgt cgatcagatc ggcgagtgcg
     4561 gcgacgtcga tctggccgga gtcgtcgttc gggaccacca caacgtcggc ccctgtccgc
     4621 tgcgcgagct gcaggtaggc caggacggcg ctgccgtatt cgttgcggcc ggtgaggatc
     4681 cggtccccgg gctcaagcgg caccgagtag cccgccgcgt tccaggcgtg ggttgagttg
     4741 tcgaacagcg caatctgcga cgggtgcgca ccgacgagct cggcgagcga ttcgtatacg
     4801 tcgccgatct gctcggccgc ctccgcttct gcttcgtacc cgccgatcag cgcttcgcgc
     4861 tgcaggtact cggtcatcgc cgcgatcgtc gccgtggtca ttaacgcggc gccggcattg
     4921 ttcaggtgat tgcggttcgc gcaaccaggg gtgagtgcgc gcagggcttc gacatcaagg
     4981 tcgggcatga gccggacatt actgtgaacg gggtgagcgg gttttcgttg ccggtggtgc
     5041 cgcgctacgc ggaggtcgac cagcaaggcg tggtgttcaa cggccactac ctcacctggt
     5101 tcgacgaggc gtgtaccgga ttcctggatc acctcggggt ggcttacccg gatttgatcg
     5161 ccgccggcca cgacatccaa gtcgtacaca gcgagatcga ctatctcgca ggggttcggt
     5221 ggcgtgactc ggtgcgagtc gccgtgcggt gtgagcgcat cgggacgacc agtttcacgc
     5281 tcgggttcac catccttcgg cgcaccgccg acaccgaaga gcagaccgcg atccgcgggc
     5341 agaacgtcta cgtggtggtg tcgaccgacg actggaccaa gcggcagatt cccgatccgc
     5401 tacgcgaggc gctgacggcg tacgactgac atgaccgaga atcgggtccg agcgggttac
     5461 gacgccgtcg cgccgaccta tgccgagcag ttccgcgacg aactcgacca caagccgctc
     5521 gagcgtggct tgctcgccgc attctgcgac ctatcacctc ccggcgtgat cgccgatatc
     5581 ggttgcggcc caggccatat cacgcgattc ctcgccgctc gacgcgacaa cgtcatcggt
     5641 ctcgacctgt cgccggccat gatcatggtc gcgcggcaac gcaattcggg tccggctttc
     5701 gccgttgggt ccatgctgca tctgccgtac cccgacgccg ccgtagccgg cgtggtggcc
     5761 atgtattcga tcatccacct gagcatcgaa gagcgggcgg tggcgttcgc tgagttcgcc
     5821 cgcatcctcg gcccgcaggg cgttttgctg gtgtccttcc atgtcgacgg gcccgggctc
     5881 gcaccgggtg acgtcaacca cctcaccaca ttcctgggtc atcccgtcga tatggatggg
     5941 tactttctga acccaacgat gatcacgagg gagttgttcg ccaacggatt tcgtgtcgag
     6001 gcgcgactcg atcgcgagcc catcgccgac atcgagttct ccagtcgacg gtgctacatc
     6061 ctcgcccgcc gagcggtacg gtcatcgcat gggccacgag catgaccacc agcgcgacct
     6121 tccgcccggc atggccgagc aactcgactt ggcccacgcc ggaatggtgt cgttcgggca
     6181 ccgcccgttt ctgaccgaag ccgaacaatt ggagtcgtgg cgacccgacg tcgcgattgt
     6241 cggggctcca ttcgacattt ccacgaccaa tcggccgggt gcccgcttcg gcccccgcgc
     6301 gattcgcgcc acggcctacg agcccggcac ctatcacctc gatctggggc tggagatctt
     6361 cgactggctg gaggtcgtcg acttcggtga tgcgtattgt cctcatggcc aaaccgaggt
     6421 gtcgcacacc aacattcgcg aacgcgtaca tgcggtggcg tcgaggggca tcgttcccgt
     6481 catcctcgga ggtgaccact cgatcacctg gccggccgcg acggcggtgg ccgacgtcca
     6541 cggctacggc aacgtcggca tcgttcattt cgacgcacac gccgacacgg ccgacgagat
     6601 cgagggcaac ctggccagcc acggcacccc gatgcgccgg ctcatcgagt cgggtgcggt
     6661 gccaggcacc catttcgtcc aggttggcct gcgcgggtac tggccaccgc aggacacatt
     6721 cgagtggatg ctcgaacagg gcatgacctg gcacaccatg caggagatct gggaccgcgg
     6781 cttcaaggac gtgatgcgcg acgcggtcag cgaggcgctc gccaaggcgg acaagctcta
     6841 tgtctcggtc gatatcgacg tgctcgatcc cgcacatgcg ccgggcaccg gaacgccgga
     6901 gccaggcggc atcacgagcg ccgacctact gcggttggtg cgtcaacttt gctacgagca
     6961 tgatgtcgcc ggcgtcgacg tggtcgaggt cgcgcccgcc tacgaccatg ccgaactgac
     7021 cgtcaacgcg gcccaccggg tggtgttcga ggcgctcgcc ggtatggccg cgcgacgccg
     7081 cgacgccgca caggcaaaac ccggcccgcc cgcgaggtag agcggccctt gatgagggcg
     7141 aagtctatcg agaagtgctg tcccccaagc tgattcgaat agtcgcgcgc gagccgagcc
     7201 ggctgcgtta gccgggctta cggttgcaac ggaaaccacc ctttcgtggg ccgtcgccaa
     7261 tcgcggtaca gccgccgatc ttggtcgcat actgcttgtg gcagccgcac gtgcggcgcc
     7321 acagggggta atggaaatga aatatctcgt tcagtggacg gcgtcatcac cagaagcgac
     7381 tcgggctacg gccaagcact tcctggaaac cggcggcaag ccgcccgatg gcgtgacgca
     7441 gctcggtcgg tggttcggca tgaacggcac gggctgcgct gtcgtcgagg ccactgaccc
     7501 caagggcgtc ttcacgctga tttctgagtg gcaagagttc atccagatca cagccacgcc
     7561 agcgctggag gacgacgagg caggcgaagt tatcgccaaa ctctacgggt agaccgaacg
     7621 gcggtcctgc tagcgtgcgc tcgtggacct gctggatcgg ctccgccttg atgttccggt
     7681 cgcccaggcc ggaatgggcg gtgggttggc gggtcccgag ctagccgctg ccgtcgctgc
     7741 cgccggaggc ctggggacgc tcgggttgct tccgccggaa gagctgcgcg aggcgatcgg
     7801 ccagctgcgg gccgccgcac ccgatcgcgc tgtggcggtc aacctgctga tgccgtttgt
     7861 ccgccgcagc cacgtcgaag tgtgtgtgca gtcgcggatc cacgtggccg tcatcgcgtt
     7921 cggcatggat cgggccctgc tgcgatcgct ctgcgaacat ggcgtgttcg cattcgtgat
     7981 ggtcggcacc gaagcgcagg cacgccgggc gttggaatgc ggggccagcg gactgattgc
     8041 ccagggctgc gaggcgggcg gacacattgc gggtgacact cccgcgctga ccttcttgcc
     8101 gcgcgcgctc gaggtggcct gtggccggcc ggtgctgctc gccggcggca tcgcggtcgc
     8161 cgacgacacc cgtgcggcgt tggcggccgg cgctgcgggg gtggtggctg gcacccggtt
     8221 cctgctgacc gatgaggcgc gtgcgcaccc tggataccag caccggatcc tggaggccga
     8281 caggacgttt cggacgatgt tgtttggcct cggctggcct gcgccgcatc gcgtggtcgc
     8341 caatgccgcg acccgccggt ggtgtcacgc cgacgggcgg agcaagctcg tgccgcggct
     8401 gatcaatgcc ggcagcggtg tgctcgccaa gcttcccgac cgcgcgatca cgcccgtctt
     8461 ggaaaagcag acgcctggcc tgccggtgtt ctcccccatc gcaccgacga tcgggatgcc
     8521 cgattcggcg gtggaccgca tggcgctata tgcgggcgaa acggtgctgc gtctcacatc
     8581 tgtgatctcg gcccggcagg ctgtcgccga gctgacccca gggtgaacaa tgaaggcatg
     8641 accggtgttc tgttcttcga cggggcctgc gggatgtgca cgcggtcggt ctatttcctg
     8701 gtgcgccacg accggacggg ccacgtccag accgagccat tgcagggcgc gagagtggcc
     8761 gagcgccttg gcattcgcga gcaccagctg ctggaagcca tgcggtggct cgactcctcc
     8821 ggagcggtct acaccggcgc cgaggcgtgg gcggctgcgt ggtcggtcgc gctcggtaac
     8881 cgactgcctc tgctgatcta ccgcgtccct ggcatccgga tcattcagaa cgccgtgtac
     8941 cgctgggttg ccagccaccg ctaccgattc cccggcacga ctccgtactg cgagtcgcac
     9001 cccgttgcct gctgacgacg cgggatgtgt ggtgcgccag agcgatatcg tgatccggcg
     9061 caccgttgca ggcgacctcg aagacatcga caggatctac gcccaccacg tgcaaaccgg
     9121 cgtcgctacg ttcgagctca ccgcaccgga tgcggcggaa tggcaccgcc gccttgacgc
     9181 cgtcatcgct gccgggctgc cgttcctgac cgcgacaaga tatggccggg tcgccggata
     9241 tgcgtactgc gcgccgtgga aaacgcgacc ggcctatcga cacaccgtgg aggattcggt
     9301 ctacgtcgca cccgatgcgg tcggctgcgg gatcggcggc cgacttctcg acgcgctgct
     9361 caatgactgc acccgcgccg gcgttcgcga ggtgatcgcc gtcatcgtcg acgccgacgg
     9421 cgcggcgtct ctggcattgc atcgcaaccg cggattcgtc gacgccgggc ggctcaccgc
     9481 agtcggcttc aagcacggcc gatggctgga caccgtgctc ctgcagcgaa gtctgtcctg
     9541 atctgcgtca gctgagctgg tcggtgactt cccgccaggc ggggttgtcg ttgcggtgat
     9601 cggcgacgtt gcggcattca ccgtacagcc gcatagtggc gaagtcggtg tcgttgccga
     9661 tgcgatagaa gaccgcggtt acgccgtccc tggtcagctt ttggccgaag tgctcgccca
     9721 tcgccggggc cgtcgtccat ccgtgggtgg ccagggcagc agcgacgtcg cgcaaatact
     9781 tgaccgaatt gccctgcggc agacggaaat tcatgtagac agtgacctga tagggcggct
     9841 cgttagcgtt cttgcatgac acgaaggcgt agccgcccgt cacttcgcgc agttcggccg
     9901 tgctgacgat ctgtttcgcc gaacccagca cctgagccgc tgcctgcgcg tcggtcatcg
     9961 gctcgccctg gactctcatc tcgtcgtctc cccgaccgct gagccacaac agacccgccg
    10021 ccaccgcaag cagcgccgcg agtgctgtga gcaccagcac cgcggattgc gacggatgca
    10081 gccgactgtt cattggagcc tcgaactaaa tctacgacag gtctgacact tgagactagg
    10141 gtatctagtg tctagtatca cagtatggac accgtgatcg tccaggcagc gatggccgaa
    10201 gccgcggcga cgggtacccc ggtcgccgag ttgtcgctgg atcggatcgc ccgtcgggcg
    10261 ggcgtgtcgc gttccaccgt ctttcgccgg gtgcgcagtc gccgggcgct cgatgacgcc
    10321 gtgcgcgagg ccggcggcga tcccggcacc cgcatcgggg tgcgcgaacg ggcgatcgct
    10381 gcggcgaccg aactgatcgt cgccgacggt gtcgccgcga tgacggtgga ggcggtgacc
    10441 cgccgcgtcg gctgtgcagc gacgtcgatc tatgccgcct tcggtgatcg cgaagggctg
    10501 ttagccgcgg tgttcgaccg ccacgcgccc ctgcctgtcg ttgaggagct gctcgccgcc
    10561 catccgcggc cgttcgccga tttcgagtcc ggcgtgcgcg ccatctacac cgcagtgttc
    10621 gacagcgttg tcgacgacac tgcggtgctg caggcgctgt tcgccgagat cctcgccaag
    10681 ccgaacggca ttggcacgca gttcttccgc gatcggatcc tgccgcgcat cacctcgacg
    10741 gtgggcgcgt ggctgaatga gcaaatccat gccggtcggt gcgccgacct gcccctgtcg
    10801 ctgctgatcc cactgctgat ctccccgatc agcgtccatc tgctaacccg acctcggctg
    10861 atcaacgcgg gtcagcctgt gccggaccgg cacgccgtca tcgaggccat gacgtccgcg
    10921 ttctgcggcg ctacggccac cacagcccat cgaccagcga gtacatcatg aaatctgttg
    10981 tcgtcaccgg atcgtcaagc ggcatcggcc gagcatgcgc attgatgttg gaccgcaacg
    11041 gcttccgcgt cttcgcgggc gttcgtaagg atgccgatgg cgacgcattg cgctccgagg
    11101 catcggggtc gctcgtaccc gtgcacatcg acgtgaccga cgctgcctcg atccactcca
    11161 tggctgacca ggtgaaagcc gaagtcggtg aggccgggct gcacggcctg gtcaacaacg
    11221 caggcaccac gctgccgtgc ccgatcgaat acctcccgct cgacgggttt cgccgccagc
    11281 tcgaggtgaa tctcgttggc ccgctggcgg tcacacaagc actactgccg atgctgcaaa
    11341 cggctcgggg tcgcgtcgtc aacgtgacat cgggcgcggg caaggccggc gtgccattga
    11401 tggccccgta tgtgtccgcc aaacacggct tggagggtct cagcgacgtg atgcggctcg
    11461 agttccgcca gttggggatt caggtcgcgg tcatcgagcc cggcttcgtt ggcacgtcga
    11521 tgcgtggcaa gctgcaacgc gacaccgagg ccgcgctgac ctcattgccg gacgagggca
    11581 gccggcgcta cggtccggcg ctggagaagc tggcggcgga gatcagccac catgcgacga
    11641 cgggctcacc gccggacgtc atcgccgaat ccgtgctaca tgcgctgacg agcaagaagc
    11701 cgcgcacccg atatccctcg ggcgcgggtg cgaggcaaat gctgttcatg cggcgcatcc
    11761 tgcccgatcg gcgattcgac cgcctcatcc tgcgcacggc cgggctaacg ggcttctgat
    11821 tccttaggcc ggtcggccaa ccacttggcg gcgcaacgac ttcggcaggt ataccgcacc
    11881 cggaccagca cctgatgcgc tgtcggacgg gttcgagatc gcacaacgct tcagcgacag
    11941 gcatccgcag ccgatgcacg aatcgagctt gtcgcgcaac gcgatcagcc ctgcgatttg
    12001 ttcatctaga cgttgtcgcc actccttcga cagccgggtc caatccacgc gcgtcggtgt
    12061 gcgaccgtcc ggcagcaact caagcgcagc tgcgatctcg tcgagggtca ggccgacgtt
    12121 acgggcggcg cggatgaatg ccagccgccg caacatgttt cgttcgaacc gccgctgccc
    12181 gccgctcgtc cggctagcgc tgatcagtcc ttgcttgtcg tagaaccgga tcgcggacgg
    12241 tgcgaaaccg ctgcgctccg ccacttcgct gaccgtcagt agatcgtgac tctccatcac
    12301 ttgctccccg ggtcttgact taaagttaac ttcaactacc agtatgcctg gtatgactac
    12361 ttcagaacaa acttctcccg tcgcgatcgt caccggtggc tcccggggct tcggtcgcgc
    12421 agcggctgtg gccctccttg accgggggtg gaccgtcgtg gcagacgcca ggcgcgccgg
    12481 cgagctcgag gcaatggccg acgaagtcga ctcaccgcgg ctgatcacca ttgccggcga
    12541 cgtcaccgac acggaccacc gggccgcact ggtcgacggc gcgacggccg ccggaccgct
    12601 gtgcctgctg atcaacaacg ccagccggct cgggccgagc ccgcaacccc gccttgccga
    12661 ttacctcgcc gccgacctgc gcgccgtgta cgacaccaac gtcttcgctc ccctggccct
    12721 gtttcaggcc gccttaccgg cgctgacgga caaccgcggt gtcgtcgtca atgtcacctc
    12781 ggatgcggcc gtcgagccat acgagggatg gggtggttac ggatcgtcga aggccgcgct
    12841 cgaccacctg tcggcgatcc tcgccgcgga gaatccggac gtcggcgtgt acgcattcga
    12901 ccccggcgac atgcgcaccg aaatgcacca gtccgcgttc cccggtgagg acatctccga
    12961 ccgccccgaa ccggagtccg tcgtgcccgc gctgctgcgg ctgctcgaca cccggcccgc
    13021 gcccggccgc taccgcgcac aggatctgct cgcgatcggg gccgacgcat gatcctcaag
    13081 ccgcagacgc aattcgagcg ctcacccgcg tccgacgccg tcgaaccgcc cgaagcgcgc
    13141 gggatgcccc gcgatggcgt caagctgatg gtcgccacgc cggacgacgt cgtgcacacc
    13201 cgcttcgccg acctcggcca gcacctcgtc cccggggacc ttctggtcgt caacaactcg
    13261 gcgacgcttc ccgccgcggt cgatgcgcga agaccgaagc gaggcccgat cgccgtgcac
    13321 ttctcggccg ccctcgacga ccacctgtgg gtcgtcgaac tgcggccggc caccaatgcg
    13381 accgcccacc tgccggacgt cgagccgggc gagcgcatcg ggctgcccga cggcgctgca
    13441 ctcgtcgtca tcgcgtccta tccacaaccg ggtgtggcgg gtagccggct gtggtccgcg
    13501 cggttggcga tcgaaggtgg cgtgacgacc tacctctgca ggcacggccg gccaattcgg
    13561 tacagctaca tcccgcgaga gtggccgctg agcagctatc agaccgtgtt cgcccaccgg
    13621 cccggcagcg ccgagatggc cagtgcggca cggccattca gcgccgagtt ggtcacatcg
    13681 ctggtctcgg cgggcatcgt catcgctccg atcactctgc acgccggggt gtcctcggcc
    13741 gaagagggcg aaccgccgtc ggccgagcga ttcgagatgc ccacatcgac cgcacggctg
    13801 atcaatctga cccgggcatc gggcgggcgg gtgatcgccg tcggcaccac cgcaacccgc
    13861 gcggtggagt ccgccgccga taccttcggc actgtgcgcc ccgcgactgg ttggacggat
    13921 ctggtgctcg gaccggaccg gccgccccgc gttgtcgacg gcctgatcac cggctggcac
    13981 gacgcaggcg cctcacacct gctgctgctt gaagcggtcg ccgggcctgc cctggtagcc
    14041 gcggcatatc gggaagcgct cgagttcggc tacctgtggc acgaattcgg cgacagcgcg
    14101 ctgctgctgc ctatttcccc gcgatcccgc tgatcaaccg gtcgcacacg gcgtccatct
    14161 cttggcgtgc ctgatcggga ttcgttgcgc gggagacgaa caatgccgcc tcgtcgagtg
    14221 cgccgagcag cacatgcgcg gtcggccgca gcggctgttc tgggatggcg ccttcggcga
    14281 tcgcatgcgc cagcatcgcc tcgatcaccc cgaggccgta tttcaccccg acggcacgcc
    14341 accgatccca gcccagcacg gcgggtgcat cgatcaacac gatccgctga acgtcgggtg
    14401 ccgaacagcg atcgaggaaa agactggcgc ccaagcgcat cgcttccagc gggtccggcg
    14461 gttgctcgtc ggcgatctgt cgcactatgt cggcgaccag agcgcgctcg acctcctcgt
    14521 agaccgccgc gaacagcgca gccttgtcgt cgaactggtg atacagcgca ccccgggtca
    14581 ccccggccgc ggccacgatc gcctgagtgg aaacctcggt aaatccattg tcggcgaaca
    14641 gctttcgcgc agcgtcgatc aacagcgcgc gcgtcgccgc ggtgcgctcg gcctgtgtgc
    14701 gccgggcggg agactctgcg gtcacgccat cgttgacttt catacagact gcacgtaact
    14761 tccatactaa gtgtttgtaa gtctgatcga aaggatagcg aagatgccgc aggtggtgtt
    14821 ggaccaggcg acgatcgact accgcgtgtt tggcccggaa gactctccgc acccgccggt
    14881 cctgttcgtc cacggcattc ttgtcgacag ccggttgtgg gaccgcgtcg ccgagggcct
    14941 cgcgcggcag ggtttccgct gttacctgcc gaactggccg ctgggctcgc acaccattcc
    15001 cgtgaacgat ccggccgcac tctcgccgcg cggtgtggcc accttgatcc gcgacttcat
    15061 cgtcgcgctc ggcctgtcgg acgtgacgct cgtcggcaac gacaccggcg gtgggctgtg
    15121 ccaactggtc atcgacgcgt accccgagca catcggccgc ctggtgctga ccaactgcga
    15181 cgcgttcgac aagttcccgc cgttcccgtt cacgctcgtc ttcgcgctgc tgcgcgggcc
    15241 gatctccatc aaggtgctgt tcgagcagat gcggatcaag gcgctgcggc actcaccgct
    15301 ggggttcggc ctgctggttc atccggaacc gcagctcacc gcctcgtggc tggagcccgg
    15361 tcgcacggac gcccgcatcc gtagaaacct ggccaccctg ctacgcaatg tggccaaaac
    15421 tgatctgacc gacgtcgcga cccgcctcag acaattcagc aagcccgtga ccatcgtctg
    15481 gggtcaacgc gaccgcgcgt tcacacccgc gctgggcagg cggctggcgg ccgcgttccc
    15541 gaatgccacg ctgatcgagg taccgggcgc gcgaacgttc gtatcgctgg atgcgcccgc
    15601 ggcggtgatc gatgcgatcg cgaaagtggg cgcgaccgcc tagctcttga ccagcgtgaa
    15661 ctggcagacg tcggtgtagc cgtcgcggaa caattcggcg cagccggtca ggtacttcat
    15721 gtaccggtcg tagacctctt cggactgaat cgcgatggcc tcgtccttgt gcgcttcgag
    15781 cgctgccgcc cagatctcca gcgtgcgtgc gtagtgcagc cgcaagcgct gcgcgcgctt
    15841 gagttcgaag ccgaccttgg cggcgtgctc ctcgacggtc gtcggcttcg gcagatcacc
    15901 gccggggaag atctcatcca tgatgaactt gctgaacttc agcagcttca tggtcagcgg
    15961 caacccggtc tctgcgaact cttcatcggt gggcttgatg atggtgtgca gcatcatcac
    16021 gccgtcctgg ggaaggatcg cgtaggcctt cttgaagaag tcgtcatagc ggtcgcggcc
    16081 gaagtgctcg aacgcgccga tcgacacgat ccggtcaacg ggctcgtcga actgctccca
    16141 gccctgcagc aacaccttct tgcttcgcgg actgtccgag gcggcgaaaa cctgctcgac
    16201 gtgagccttc tggttttcgc tcagcgtgag accgatgaca ttgacgtcgt agcgctccag
    16261 cgcgcggttc agcgtcgcgc cccacccgca cccgatgtcc agcaacgtca tgccgggttg
    16321 caggcccagc ttgccgaggg acaggtcgat cttggcgatc tgcgcctcct cgagcgtcat
    16381 gtcgtcgcgt tcgaagtagg cgcagctata cgtctgggtc ggatccagaa acagccggaa
    16441 gaagtcgtcg gacaggtcgt aatgcgcctg gacgtcctcg aagtgcggct tcaagttctg
    16501 ggtgtcgctc gtggtttctg gcaagttgca gccctttggc gtctggagtc gatgtggtcc
    16561 gggctccgcg tgttttgaga gcctcccccg tcgtgagggc agtgtacgca ccctcgatgt
    16621 gaacggctcc cagagacctg ttcgacacag atagttgaca atatcagcga attgtttgac
    16681 gctatcaact atgtccagtg tggtcgacga cgtgcgcggg ttcaaccgct tctacacccg
    16741 gatgctcggg ctgctccggc cccacctggc tggatcagcg ttcgggctca ccgaggccag
    16801 agtgctgttc gagctggcgc atcgagatga cgctgcggtg tccgaccttc gtcgtgagct
    16861 cgatctcgac gccggctacc tgagccgcat cctgtccagt ttcacctcgt ccgggttggt
    16921 cgtccgggag aagtcggcgg ccgacggccg tcgccaggtc gtgcgcctga cggacgacgg
    16981 ccgtcgcgcc ttcgacgagc tcgatcggtt gcaggccgac gcgatagcga ccatgctcac
    17041 gccgctcgac gacggccaac aggccgaact cgtcacctcg atggggcgaa ttcgccgcgc
    17101 gttgggtgac gaaccgcgct caaagggcct cgtccttcgc tcgccagcgc cgggcgacct
    17161 cggctgggtg gtcgagcggc acggttaccg ctactcggcc gagtacggct gggacaccga
    17221 ctttgaggcc ctggtcgccc gcgtcgtggc cgactttgcc gaacgcgacg acaccaaacg
    17281 cgaggcggcg tggatcgccg agctggacgg agagcgggtc ggctgcgtgt tctgcaccgc
    17341 cgcggacgtg aaggacaccg cacagctgcg cttgctgctc gtcgagccgt cggcgcgggg
    17401 agcgggcgtc ggcacccggc tggtcgatga gtgcctgcgg ttcgcccggc gatccggcta
    17461 tcggcgaatc accttgtgga ccaacgatgt attgctggcg gcgcgcagga tctaccagcg
    17521 cgccgggttt cactgcgacc gcagcgagcc gcaccacagc ttcggccgcg gcctcgtcgg
    17581 cgagtactgg tcccgggacc tgtaacgcgt caggcggccg gcaccgcgcc cagcacccgc
    17641 tggatgtccg gcttcatggc ctgtaactgg ccgccccagt aaccccagct gtgcgtgccg
    17701 ttgggcggga agttgaacac cccgttgctg cccccggctc gcgtgtaggc cgcctggaaa
    17761 tccttgttgg tgcggatcgt caggccttcc aggaactgcg cgggaatctg cgcattgccg
    17821 agcccggcat ccagatccgt cggattgccg ttgccgctgt acacccagat gcgggtgccg
    17881 ttggaaacca gtcgcccgat ctgcagcatc gggtcattgc gctgccaggc aggcccgccg
    17941 tacggacccc acatgtcgag cgcgttgaaa ccgcccgcgt cgcccatcga gatgcccacg
    18001 agcacgggcc acagcccccg cgaaagattc aggaagccgg acagcgaccc ggcgtagacg
    18061 aactgtccag ggtgataagc cgcgaggatc agcgcggaac tgcccgacat cgaaaggccc
    18121 acaacggcgt tgccggtcgg gctgacgccc ttgttcgcgg caagccacgc cggcagctcc
    18181 gacgtcagga aggtttccca cttgtaggtc tgcgtggtgc cgttgcccac ggcaggccgg
    18241 taccagtcgg tgtagaagct ggacatgccg cccaccggcg ccactactga tacgccggag
    18301 ccgtcgaacc aaccgaatgc gccggtctcg atgtcccagc cgttgcgatc gtcgcgggcg
    18361 cgtagaccgt cgagaagata gacagccttg ggaccaccgc cctggaattg aacggtgatg
    18421 tcacgaccca tcgacggcga cggaacgctg aggacttcga ccgccgcgcc ggccgttgga
    18481 gtacccccga tcaccccgac cagcccgggc agcagtgccg ccgctgccat cgccgccgcc
    18541 atccggcgga cccattttct tggtgcctgg tgtgaaacgt tcatagccct tcatcccgtc
    18601 gtgtgtatac ggggttaatc gcggcccaag aagcctcagt tacagggaga tgggaggaca
    18661 atcgaacttg cgtatcgacg gaccggaaac gaaaggggcg cacgatgaag gccaaggtgg
    18721 gcgacttcct ggtggtcaag ggcaccacgg ttgagcagca cgatcagcgc ggggtgatca
    18781 ccgaggtccg gtcggccgac gggtcgcccc cttatgtggt gcggtggttg agcaccgacc
    18841 atgtcgccac cgtgtatccg ggtccggacg cactcgtcgt gacgcctgcc gagcaggaag
    18901 cggccgacgc gcggcaccga gcgcactagt cgcaagatgg acggagaccg atcatgacga
    18961 agcccagcgg tgagttcggc gacgacgctc ccgaagccga cgtgatcgaa caactccgct
    19021 ccaccgacgt cagcgaagag gacacgtggc gggacgcggc ccgcgtagcg agtgcacgcg
    19081 actgggaagc caacgaggcc gacttgatcg agcaggccat cgccgttccg gatgatgatt
    19141 cggagttcga ccggtgaact cagttgtgca gggctgacga ctccttgaga agcagcccat
    19201 agccgttgca gcacaggctg acccgcaaag ggtggctagc gttgtgcgtc gcaagccagg
    19261 cgcgcagcgc cggcttctgt ttctcgaccg aagcccgctg aaccccgagt tctttcagca
    19321 gcgcaggcag ggtggtgggt tccgctgtca cggccatggc tatccccctt tttggcccga
    19381 cagtcaattc taaatctggc cgccccgccg cgtaccgatt tgcccaaaag ccttgccgca
    19441 ccgggcggca gcctgtcgac gttagccggg gacgcgggca tgccgccgga ggcgaatgat
    19501 gtagccatga ggcttgatgg atacctccgt cggtgcgcgg ccttggtcgg tgcgcttacg
    19561 accctggcgg tgatgccgac gaccgcgccg gctcgcggcg acgacgccaa cccgctcctc
    19621 gaccttgtcg atgcggccgc ccaacggctg caaactgcag agccggtgtc ggcgaccaag
    19681 tggatcaacg gcggctcgat cgacgatcct ccccgggtcg agcaggtatt ggcagcggtg
    19741 agtgccgacg cgacgggcag gggcatcgac cccgactacg tcagacagat gttcaccgat
    19801 cagatcgatg ccaccgaagc catcgaatac acccgattcg ggcaatggaa gctcgatccg
    19861 ggatcggcgc cacgcattgc gcccgacctt gcggcgtcgc gcgcgaccat cgacggattg
    19921 aacaccgaga tggttgagca agtcgcgctg cgctggtccg tcctgcactc ccccgactgc
    19981 acccgcatgc tcgacgacgc gacgaccgcc gtcgccagtg cgcggatgct cgatccgctc
    20041 tatcagcaag cgctcgcatt ctccagtcag tcatattgcg cgccttgatg tttcacatca
    20101 gcgttctctc ggcggcgatg tcatactcgg caacggaggc ggcgacgatt gccgcgccct
    20161 gatcgtcgct gggaccgctg ccccgaaacg cttcgaccgc ggcctgcgat tcccaccgtt
    20221 cgaagatgtt gacccggccc ggctcgagca ggtcggccga cagcgcgaag tcgagacatc
    20281 cggctgtgcc gcgggcctgg cgcaccacct cgacgcatcc cgacaagtag tcgtcgcgtt
    20341 gctgcggatc gaccacgatc tgtccagcga caatcaccat cgcgggcctc cttcgtctgt
    20401 tcgattgaac tatcacctga ttgactgcag ttcgagtcag aaatcatcgt cggcggtagt
    20461 tatggaggag ccatgaatct tcgagaggca cgacaagtca tcggctcgct cgagtccgat
    20521 cccgacgccg cgcgcgtgtt cggagaggcg taccagacca ccgatggggc aacggtcatt
    20581 ccggtcacta ctgtgcgcgg cgcgtccccc aagccggccg gcgtgttcgt cgttaaggac
    20641 ggcaaagcgt cgtgggtgcc cgctgtcgac gcagggcgca tcgcgatgtt cggaattctg
    20701 gttggcctgg tgtcggcaac gcttgccggc gtggctatgg ttcgtcgtcc gccgtggccc
    20761 gatctgcacg gcgatatctc gagccggcgc tagcacgcca agctgctgcg aacacaatca
    20821 gcacggcaag cgcgacaagc catggccagg ctccgtgctg gtggatccag cccgcgatcg
    20881 caccctgcac acggcctgcg gccgcgatca ccgggtcttc gggattgccg ttggcggtga
    20941 agagccggat ttcatagagc ccgtaatagc cgacatagag gccgacaatc acgagcagca
    21001 cgccgctgat ccggttgacg tacggcacaa ttcggcgcat ccggtcgacg gtggcggaac
    21061 tcgccagcgc tgcggccatc gccagcactc cgacgacgag tgtgaacccg cctgcataag
    21121 cgacgtacac cgccatggcg ccgagaacgg aaccgccgcg aagcccggcg gctgtcaccg
    21181 cgagaaacgg cccgaccgtg catgataacg acgcgatcgc gtagctgatg ccgtagccga
    21241 acatcgaacc cagtcgggcc gtcggcgccc accgagcgcc agctttcatc ccgcccagca
    21301 ccctgatctc ccgacctgcc aacagccaaa caccaagggc gacaagcaca atcccgatca
    21361 gcacggtgac gtacggcaga tagcgctgca ccgtcgcggc ggccgacacg gtgagcacgc
    21421 cgaacccgcc gaacacgacg aggaatccgg cggccatcgc ggtggtggcc gtcaccgctc
    21481 ggccgatcgc ggcacgccgt cccaaatcct cgccccggac cgcgagggcc agatacgccg
    21541 gcagcatggc gaagccgcag gggttcagcg ccgccaccaa tccagcgccg aaggccaagc
    21601 ccaccaggtc ttggtccacg tgcgcggcga cctatgacag cgccgctacc cggtcagaca
    21661 gctcctgttc gggcatggcc gcggtcgggt tgttcacaaa cgtcgacgag ccgtcggccc
    21721 ggtagaagac gtaggccggc tgccacggga cgttgtagcg ggcccagatc gacccgtcgg
    21781 cgtcgttgag gttggtgaag gtgaggttgt acttcgacac gaaggcctgc atcgccgcga
    21841 cgtcggagcg cgccgccacg ccgacgaacg tgaccttggg attggccgcc gcgacctggc
    21901 tcacgctggg cccttcggcg ttgcagaacg gacaccacgg cgtccagaac cacagcaccg
    21961 cgggcttgcc gatcaggctc gccccgctga acggcgcccc ggacagcgtg gtgccggtga
    22021 actgcagccg gtcatctgcg accgccgcag gtggactgac aagcccgaac gccagcgcgg
    22081 ctgctgccgc gctgagtaca agcgatctga ttagtcgcga gaatcgagtg ctcatccccc
    22141 atctccttcg ctcaagcggc accgcgtgga tgacctgcgc cccacacttc taaacacgac
    22201 cccagttgcg tcggggttca cgtccagaac ggggccagat gtcgctggag gcttggctgg
    22261 ctaacgtgtt tgggctcaag ggccaggatg cctggcggcg ccacgccaat ccgtggagcg
    22321 tctacacgcg gattccgatc ccagcggtgc ttgtcgcggc gattcggagc cgaacatgga
    22381 tcggttggtg gtctctgctg ccgatcgcag tgtgcctgat ttggacagtg gtgaatccgc
    22441 gcgcgtttcc gccgccgcgc acgctggaca gctgggcgtc ccgatcggtg atgggcgaaa
    22501 ctctatgggc cgagcgaaag tcgacgccgt tgccgtcgcg tcatcgcacc gcacccgtgg
    22561 tactcggtgc cctcagcgcg gccggattgc cgttcctgac ttggggtctc gtggtgctcg
    22621 acccgtggct cacgctgttc gggctggcga ttcagatgct gggcaagctg tggtttctcg
    22681 accggatggc gctgctctac aacgacgtca gttctcgcgc tgcaccaatt ccgccacgaa
    22741 ccgatcgatg aaatcgtcga tgttcgccgg cagcgcgggc cggatcacga acttggtcaa
    22801 acccgctgcc aggtacgcgt cgacttggcg gtgcagttgc gaccatccgc tcgcgatgag
    22861 gtcgcctggt tcgacgtcgg gcctgcgccg cctgaccgcg gcaatcaacg cgtccggtac
    22921 gtcgccgtcg ctgacggcga ggttgatacc gaaatgatcg ggttcgatct cccggcccgc
    22981 gtcggtggcc gcccgctgga tctgctcgcg gccggcgcgc gcttcgtcgg gcgtgaggaa
    23041 gctaccgagc cacccgtcgc cgagttggcc gatgcggcga aacgcggccg gcgcggatcc
    23101 gccgagccag atgtcgagcg gtttgaccgg tcgcggctcg atgaccgcgg aactcacagc
    23161 gaaatagtcg cccgcgaatg cgacatcctg ttgttccaga accgatctca gcagccgcag
    23221 cgattcatcg aagacggcgg cgcgctcacc gtcgggcacc acgaagatct cgcgctccgc
    23281 gggaatcgcc gaccgcaggc cgaacaccgg gagcacgcgc ttgggtgcca gaccggccag
    23341 cgacgccaac tgcttggcca ccagcacggg atggcggccg ggcagcaccg cgaccgacgt
    23401 gccgaccttc aggttggtcg tccgggccag cgcataggcc atcccgacga acgggtcgac
    23461 cgccttggag tacaccaatt cggaaaacca cagcgaatcg acgccggtgg cctccagatg
    23521 gtcgacgatt ctcgccagtt gctccgggcc tgtatccgca ccgagcccga cgccgaaccg
    23581 aatcttcacg acccgatgct acggaaactg ggcgaaaggc catgtgcagt tgcacgacgg
    23641 gccagagtca tggactcgtc cccgaaggaa tgggcttgtg gcttgtcctt gaggtttcga
    23701 gtctgccgtt aggcttgctg ctgccgccct agctggctgc cagggtgggc gctgcgtatg
    23761 gacgcatgac ttcgccccag cgttcgacac cgcggtgagc aatgacaatc gctgcattgt
    23821 ggtcggcgtg cccaacgaag ccacatcgac gacactcaaa ctcgctctgc gagcgacgat
    23881 tacgtttgtc aacccacccg cattgatggc aggtttgact cgtgtacgcc gggtcgacct
    23941 cgacgaacgg cacgcccgct cgatttgcct tgtaggcgac aaatccgccg agttgggcga
    24001 acgcccatga gtgcagcgtg gcccgttggg gctttcgaag ccgtacccgc gcacgaatcc
    24061 ccgccagttt ttcgacggag ataccgcgcc cggtgcgttg cgcctcggcc acgatgcgtt
    24121 ttgatacttg gtggttgagg tcggtggcga accgcgcctc tttgcggcga cgcttcttga
    24181 gcaaccgccg agccgaatta gtcttctttg cctgcaaccg ctgtcgtagc cgctgctggc
    24241 gtttccggta gcgggtcagc cgcgcgccgc tggcacgttg cccgtcgctg gtggtggcga
    24301 tgttgacgat cccccgataa acccattaac cggatcggtc aacggcgcct gcggcacctc
    24361 gacagcggca tacaggtacc aattgccgtc ccgatagatg agatcggttt cgccgatcgg
    24421 gtgggtgcgc agcattgcga gatcccgcgt tgctgccaag acacgtacac ctttgcaccg
    24481 gccgcgggtg gtccagatcg acaccgtcgc tgtacggccc ccggcggttt cgggtatctg
    24541 ccacgacaag caccgagcat cgaacggctg ggcggcatcg gtacggaacc ttacgggcgt
    24601 gtcctgaact gcctttcgct tgtccgcgct gggtgggccg aaattgcccg cgtcgatgct
    24661 cgcccgaagc gtgcggtacg catccacgac cttgccaatc acccggatcg ccggctgcgc
    24721 cgataggccg aagcgctgct tgagttcgcc gtagaactgc tgctgcgcat cggccttgcg
    24781 gtgcacgtgc gttgcatgca tcgcagccga caaccaggat gccgcgctgt tgcaggtgcg
    24841 cagggtggct tgtagcgcgg ccgcctgttc gggggtcggc agcatgcgca ctttgaccac
    24901 ctgcatcatg cggacacgct aacggtcaac accgacacct ccagtctcga caaggccgcg
    24961 agcggcgcta gtagtacagc gcaagaccga cgaccttggg gcgccccgca ctccgtggcc
    25021 aacctagaca ttgagcagca caaacgcgcg aacctgcggt caacgcgcac gcgcgatcca
    25081 gtttctctcg cccgtaaacc ttcaggtatc tgccgtagta gggccagaat gaaaaaatga
    25141 accaaccgtg ggtccgtggc tgcgtggtcc agcgcatcat gttccgccgt ggcctggtgc
    25201 tcaacctcga cgacaacgaa ctcgtcatct ccgtaccgtt ccatctgacg ctcccggcga
    25261 cggtccgcga cgacgaggag gcggtgctca tcgatccgaa ggccgcgcgc gccgaagagc
    25321 gtgcgctgtt cgacttcgcg ggttcgacgt gcactcacgc cagctgggac gaggacggca
    25381 gcctgcatct ccgtttttcg gacggccatg agatccacgc gcacagcgac gcggacggca
    25441 ccgcgtggga gctatacggc aagaaccacg gctacgccgc atgcctgcca cgcggtcggg
    25501 tgcgcgtcgt caggcacgac ctacctgaag acgagtctgc tgaacgctag cggtgcagca
    25561 gggcccgcat ctgggtgaat ctgaagagcc cctggcgccc ggcaaccggc tcgtaggcct
    25621 gcacctggtc ggcgacgaac gcggcgcgat tctgtgcggg cagccgatcg gtccacgccg
    25681 tcgaccccac cgcgcaccac cggctcaacg cgtcgcggga gccgaagtcc cactcgcggt
    25741 ccgtaacggt gatcttgtcg agcgtcaggc cggccgacgc ggcgagttca ccgtatgcct
    25801 cagggtcgac gtgtgtgtac ggcgccgaga agccgtcgaa ccaccgtgcc caccgcggac
    25861 tttcacagac cgccatggtc gtcgcctcaa tgctggtgcg cggtccccca caaaccacct
    25921 ggatcagcgc tcggccgccg ggacgcagca ccgtggcgat ctcgcgtagc gcttgccgct
    25981 gctcgcggac ccagtgcaac gcgttgaatg acaccactgc gtcgaagaat tcggcgaacg
    26041 gcaactggcg agcatcggcc acgacgaacc acggcccaga gccggttggc tcgccaccgc
    26101 ggtgcgcggt ctcgatcatc cttggtgagg cgtcgactcc gaccgcgcaa ccatcgggca
    26161 ccatgccggc caccgcgcgg gtcaagtagc cgtcaccgca accgacatcg agcaccctgt
    26221 ccgccgcgcc gaaccgcagg gccgtcacgg cctcggtcgc catcgcacgt tgcggactgc
    26281 tgacatcggc gtagtcgcgg ccgctccagt cgggcatgtc acagagttaa cgcgaaatgc
    26341 ggttcggcgg cagactgctt gcgaatagtg tggcgtgcga tgagagcacg gataatggcc
    26401 ctggccgtga cggcggtggt gctagtggcg gcgtcgggct gcgccgacga atcaaaaccg
    26461 caatcgtcga ccaccagtcc cccggcgacc tccgagacag cgaagccaat gaacgatgcg
    26521 cttgcgcagc gactcgacgc cgcggtcaat caggcgatga cggcggcggg catcccaggg
    26581 gcgatcgtcg gtatttgggg tcccgacggc gactacgtgc gcgcgttcgg agtggccgac
    26641 aagacgactc gcgcaccgat gaaggccgac ttctacagcc gaatcggcag cgtgacaaag
    26701 acattcacgg tgacggcggt gctgcaactg gccgaccaag gcaagctgcg cctcgacgac
    26761 ccgatcgcgg agttcgtcga cggggtgccg ctgggcgaca agatcacgct gcgccagctc
    26821 gccaggatgc agagcggact gttcaactac agcgcctcac cggagttcca gcaggcgatg
    26881 ttcgccgatc cgcggcaccc gttcactccg cgcgagttgc tgaactacgc gctcgcgcag
    26941 cctaatcagt tcccgcccgg cgaaggattc gagtactgca acaccaacac gatcctgctc
    27001 ggcctcgtgg tgcagaaggt cagcggtcag ccgctgaacg cctacatcga ggaccagatc
    27061 cttgctccac ttggcatgag ccacaccagc tttccgacca cgaacgcgtt ccccgaaccg
    27121 catgcgcagg gctacaccgt gcagaccgcg gacggcaagg agaccaccgc caccgactgg
    27181 gacccgtcct ggggatgggc ggccggggcg atgatttcca cgctcgacga catgcgcatc
    27241 tgggcgcccg cgctggccag cggcaagctg ctcacgcccc aaatgcagga gcagcggctg
    27301 cagacggtgg gccaaccggg gatgccgccg caggacggat acgggttggg catcttcaac
    27361 ctcggcgggt ggatcgggca caacggcagc ctgcccggct atcagaccgt cgtcgtgtac
    27421 ctgcctcaga agcagaccac gctcgtcatc atgatcaaca cggacatcga gtatcaaggc
    27481 aacgagccaa gcaccacggt ggcgaccgcg attaccaaag agctgtcgcc ggaccacatt
    27541 tactccttcg gtccgcaaat gccccgttga tccagaaaga ggtgtagtca tgcagggttc
    27601 agcgaaggta accatgtcgg cgccggccga ccagatctgg aacctcatcg ccgatgtccg
    27661 caacaccggc aggttctcgc cggaggtcct cgaggccgaa tggctcgacg gcgcgaccga
    27721 gccggcgctt ggcgcacggt tccgcggaca cgtcaagcgc aacgagatcg gcccggtgta
    27781 ctggaccacg tgtcgggtca cggcctgtga gcccgggagg gaattcggtt tcgacgtgct
    27841 agttggtgac cgggctgcga acacctggca ctaccggctc accccggtcg acggcggaac
    27901 cgaggtcacc gagtcgttcc ggatgcccga caacctgttc agcaaactgt tcggggtatt
    27961 gggtggtcag ttgcgccagc ggcgcaacgt ccgcgacatg cgcacgaccc tcgagcgcat
    28021 caaagctgtt gtcgaggcgc ccgagggtgc gtgaacgagt cggttactgg aacccgatgc
    28081 cgaagaccgg gatccagagg ccgaacagcc agatccccca ctggtggaag ccgtcatccc
    28141 agacgggatt gacgtcgtac ccccagtagt tgaacggtcg cggcgggccg ccgtcccact
    28201 gatacgccgg cggtgggccc cagccccatg gcggcggacc attgcggtcg tcccaccact
    28261 cgtggcggtt gctggcccac cacgggccct tgtctccgtc ccagtgcttg aagtccggcc
    28321 cgttgccgtt gccgttgcca ttgccgttgc cgcaccaagg cggacagaaa ttgtcgtgcg
    28381 gcttcagcgg tgccggaaca actgggtcgg cggacgcgag ggcgccgccg ggcccaatgg
    28441 ctcctaagcc gagggcaccg acaatcatgg ctgcggacac gaatttcgac agcctcatcg
    28501 ttaaataact cctaccttgc ggccccgacg tccaaatccg acgctgggcc gctgctaatc
    28561 attccccaat cggagcgaag catacgtctg ctcccacggg gatatcggcc caggtgaaga
    28621 cgccgttacc gcactgaatg gcgttggcgg gtcagggttt tgcggtgact agagggtccg
    28681 caggcgacgg atacgatcgg cgattgctcg ccgcgatacg tccgctcggc gtgccagcgc
    28741 ctcgaacgcg tgcgggcaac cgggatgcag atgcagctcg gtcggcaccc cggcgtctga
    28801 caggtgccgc gcatacatga tgtcttcgtc gcggaagatg tcgaggtcgc caacgtcgat
    28861 gtaggtatcg ggcaggccgg tcaggtccgt ggccctggcc ggcgcggcat agggcgacac
    28921 gccgtcggcg cctgcgcttt cgccgaccag cgccgcccag ccggtgacgt tgtcgtcgta
    28981 ggtccaggtc aagtacggca gcaactgcgg gtcgggggtc tgcgcccgat cgtcgagcat
    29041 cgggtagatc aacagctgtt gggcgatggc cgggccaccg cgatcgcggg caagcaggca
    29101 gacccccgcc gccaagccgc ccccggcgct gtcgcccatc acgccgattc ggccgggatc
    29161 gacaccgagg gtcgcggcgt tgtcagcgag ccagcgaagt gccgcgtagc agtcctcgac
    29221 cgggatggga tgcgggtgtt cgggggcgat ccggtagtcc actaccagca tgggcacacc
    29281 ggacgtggca acgtactcgc gcaccgccag gtcgtacaca aggccgaggt gctcgaggcc
    29341 gaggatcatg cccccgccgt gcaggtacag cgccgcactg cccgggtgcc tcctcccggg
    29401 catgtgatac caaccgaggg ccaaggtcgt gccgtccgcg gtcgtcagtg tgcgcctgcc
    29461 gacctccaca tcgggaaccg gttgccacgt gcttgcgacg tagtcgaaca tccgatggcc
    29521 atcgatccgc ctgccttcga cgtcgccgat cgccagcggc tcggattctc cgaccgcctc
    29581 catcaatggg gcgagctccg ccagcacctg cgcgtcgata ccgagtgcca tggccccgac
    29641 agtaacgtgg acgcacgtga cggaagcgca aaccgacacc accaccggtc agcgcatctc
    29701 acccttcgcc gcgggagcgg ttctcccgat cgcagccgtt accaccgtat tgcattgcat
    29761 tgcaaccact ttcggtgaca gttattggtt cgacgaggtg tacatgttgg cgatcggccg
    29821 ctatcacctc gactggggat cagccgacca gcctcccctc gcacccgcgt tggccgcgtt
    29881 gaccgatgcg gtcgcacccg actcgctgat cgcgctgcgc gcgcctgccg tgctggcgac
    29941 cgcaggcgga atcgtggttg ccggtctcat tgcccgcgaa ctcggatgtg acaggcgcgc
    30001 acagggattc accgccgcgg cgcaggcgac gggcgtgtgg acgaccctgt tcggccactg
    30061 gctggcgccg tacaccctcg aacccgttca gtggctgttg gcgatctggc tgcttgtgcg
    30121 gtgggtgcgg ctgcgcgacg accgcctgct gctcgccctc ggcgtcgtcg tcggcgttgc
    30181 ggcattgacg aagttccaga ttctgctgct gtgcgccgtg ttggtgatcg ctgttgcggc
    30241 gaccggcccg cgtgacctga tgaggcggcc gatgctgtgg gcaggagccg gtatcgccgc
    30301 actgcttgcc gcgccgacgc tggtgtggca acagctgcac ggctggccgc aactacggat
    30361 ggcgcctgtg gtcgccggcg aagccgaggc tctctacggg ggtcgccccg gcatcgcggt
    30421 gcagctgatc gcgttcgcgg gcgtggcggg agcggcgctg atgctgtatg gactgtggcg
    30481 gctcttccgc gacgacgagc tgcgcgacta ccgcttcatc ggcgtcgcct tcgtggtgtt
    30541 gtacgtggtg ttcgtggtca ctgccggccg tccctactac ctagccgggc tctacgcgcc
    30601 gttggccgcg ccaggtgcgc tcgggcttca gcggcgccga gaaatcggcc ccagccggct
    30661 gcggtggctg atatggccgg gctatgtgct gagcgtggcg ctggctgtcg gcttgctgat
    30721 cctgtcggtg tcgatcgtcc gctccgacgt cggcgagcag atcgcgcaac ggaccgcgga
    30781 cgcctaccgc gcgctgccgc gggtgcaacg cgaccgcgcg gtggttttcg gcgagtccta
    30841 catcgtcgcc gcgtatctcg acggttactc gacgcgctac cagctacccg aggcatacag
    30901 cgccaaccgg agctacgggt acttcccgcc gccgccctcc gaccatgaca cgctgctcta
    30961 catcggacgc gatcccggtg cgctgcgctc gtatttcgcc gatgtgcgca gggtcggtga
    31021 cgtcggcgag gagttgcacg cgtatttgct gacgggacaa cgacaatcgt gggtcgagat
    31081 ctggccacgg ctgcgcacgt tgacggtgtc ctgaaaccgg cccgccgcgt cgacctgcct
    31141 ggtagaacag gttctagttt gtcgccgcga gccgaaggag tctctgatgg gtcaatggtc
    31201 acgcgaggaa ctcgaagacg cgtttcaggc ttaccagcag gacgtgatcg aggttggcaa
    31261 gacgtgggac tggtcgagct acgccgacca tttcaccgag gatgcgaagt acgtcgagca
    31321 cgccttgggg aacatggagg gccgcgaaaa catccgagag tggatcgtct cgaccatgaa
    31381 catgttcccg ggcagtgaga tgcccgtcta ccccgtggag tggtactcga tcgacaccga
    31441 caagggctgg gtcatcttca agaacatcaa cacaatgaag gatcccggcg acggcagcgt
    31501 gcacggagcg gcggtcatca ccgtgctcga gtacgcgggc gacggcaagt ggagctacga
    31561 ggaagacgcg tacaacccga tgaacttcct ggtgatgatc caggggtatg tcaagcgctg
    31621 ccatgaactg ggcacgctgt ccgacgacgc ccgagtgttc gccaagaaca tgaactggga
    31681 actgtcctaa cggattacga agtcatcggg atccggctcg cgggtccacg cccagtagtc
    31741 gacgagccgc caggggctca agatgtgcac ctcgccgaag ctgttcttgt agaacgaatg
    31801 cttgaccgac ggctgcgacc ataccaacct gcgcatctcg gcctgactgc gttcgtgcca
    31861 gtcggcagcc ttgtcttccc gaggctctag agaccgatgc cggccgcgga tcaatagctc
    31921 caagcactct gtgatgtagc gcatttggca ctcggagtga aagatcaggc tgccgccgtg
    31981 cgcgagattg gtgcccggcc cgtacatgca gaagaagttc ggatagcccg gcacggtgat
    32041 gcccaggtac gccgacggcc gctcgcccca acgctcggac aatactgcgc cgtcgcggcc
    32101 gcgaatcgtc atgggccaca gcatcttgtt ggcctgaaat ccagtggcgt acacgatgat
    32161 gtcggcggga tagcgctcac cgtcctcggt gacgacagca tcggactcga tgtggtcgac
    32221 acccgtgcgc accagctcga cgttgtcgcg ggtcagcgcg cgcagccagc tgccgttgtc
    32281 ctgcagggtg cgcttgccgg tcgccgggta atcgggcacc accctggcca acagctcggc
    32341 atcgtcgccg acctggctgg tgatccactc ggtgaacatg attcgggtga tctcgttgat
    32401 ctcgctgacc gccttctgct gatccgggta atccgggtcg acatacgcgg cggccagccc
    32461 cttgtcgcag ccgggccaga acagcaggaa ccgataccac cggccgtaga agggcagatg
    32521 ccggagcgcc cactgcacac cgggtcccac ctcggcgtgg tagttcggat tcgggaacat
    32581 ccattgggct gttcgttgga acacggtcag ctgctcgaca tcgcctgcga tggcgggggc
    32641 gatctgaaat ccgctggcac cggcgccgat catcgcgacg cgcttgccgc gaaggtcgac
    32701 ggagtgatcc cactccgagg agtggaacgc cggtccggcg aaatccttgt gcccgtcgat
    32761 gtccggaagg tgcgggcggt tcagctgccc gacggcgctg atgaccgccc gggcggtcaa
    32821 cgtcgtgatc gctccgttgc ggtcacgggc ctgcaccgac caggtggcgg tgtcgtcatc
    32881 ccaggacgcc tcgatcacct cggtttccca cagcacatgg cggccgatgt cgtacttgtc
    32941 catcacggtc tggaagtagg cctgcagctc gggctgctcg gcaaagaagt gggtccagtg
    33001 atcggtcggc tcgaagctgt agcaatagaa gtggtttccg acgtcgaccc gggcgccggg
    33061 ataggtgttc tgccaccagg tcccgccgac gcccgcgttc ttctcgacga tcgtgaatgg
    33121 aatgccggcc tctttgagtc gaatgcccgc cagcagaccg gattcgccgc atccgatgat
    33181 caccaccggg aagtcggcag gcgttgccgg cacggcccga gcgtcttggc cgtcgagttc
    33241 catctcctcg agcaacatcg gcacgtattc gggcggcacc ggttcgcaga ccagccactg
    33301 catcatttcc tgcaacagct ccgcgccgat cggctccggc tccgggcagc cgcggtcgcg
    33361 atagtccgcg atcacctcca gtgcgatttt gcgcaccgcg gccttgtcct cctcggacat
    33421 gtagccctgc acctcgttga ggaacaggcc cgcgggtttg agctcgccgc gaatcaagct
    33481 ggggtcgccc gacatgtgga ccagcgacaa catcagcgtc gggatgctca cgtccagcaa
    33541 cgcctccgcg atctggacgt cggagctggt aaagggttgc cctgcatgtc ggttgcgcac
    33601 ggttcggtgc tatcacgccg aacggccaat tggaacatgt ttcagaaatc gatgacggcg
    33661 gctgggtccg cccctacgct cgtgaaggtg ggtatcgcgg ctcgggcgaa cgggactgct
    33721 cccccggatg tgccactgac cgatatcgac ctgggcgact ggagcttctg ggtccaggac
    33781 gacgatctgc gcgacggcgc gttcgcgacg ctgcgccgcg aggccccggt gtcctttcac
    33841 cctgcgctgg agacgccggg ggtgcaggaa ggtccgggcc attgggcggt gaccagatac
    33901 gacgacgtct ggttcgccag ccggcacccc cacatcttca gttcgcatcc gagcatcacc
    33961 atcaacgacg cgaaccccga cgtgtccgag tacttcgggt cgatgatcgt gatggacgac
    34021 ccgaagcacc tgaggctacg caacatcgtc agtagggcgt tcaccccgaa agtcgtcgcc
    34081 cggaccgaaa cgtcggtgcg cgatcgggcg cgccgactcg tcgagtcgat gatcgaagcg
    34141 catcccgacg gctcgggcga ggtggtggcg gaactggccg ggccgctgcc tctccaggtg
    34201 atctgcgaca tgatgggcat tcccgaggaa gatcaccagc gaatgttcca ctggacgaac
    34261 gtgattctcg ggttcgggga taaggacatc gccaccgact acgaggagtt cctcaaggtc
    34321 tcgatggaca tcggcgcata tgccaccgcg ctggccgagg accgccgagc caacccgcgc
    34381 gatgacctga cgacagcgtt ggtgcaggcc gaggtcaatg gcgagcggct gtcgtcggcc
    34441 gaggtcgcgt cgttcttcat cctgctcgtc gtggcgggca acgaaaccac ccgcaacgcg
    34501 atcagccacg gcgtgctcgc gctcacccgc ttccccgatg agcgcgcgaa gtggtgggcc
    34561 gactacgagg cgatggcgcc gacagccgtc gaggagatcg tgcggtgggc atcaccggtg
    34621 atctacatgc ggcgcaggtt gaccgaagac tgcgagctga gcggcgtgaa gatggcggca
    34681 ggcgacaagg cgacgctgtg gtactgctcg gccaaccgcg atgaagccaa gttcgccgac
    34741 ccctggacct tcgatgtcgc gcgcacaccg aatccgcacg ccggcttcgg cggtggtggt
    34801 gcgcatttct gcctcggcgc caatctggcc cgtcgcgaga tcagcgtggt gttcgaggag
    34861 ctgcgccgcc agatgcccga catccaggcg acagaagagc cgtcgcggct gctgtccccg
    34921 ttcatccacg gcatcaagcg gctgccggtg tcctggacac cacctcgctg agttcggcgc
    34981 ccagctgatc agacggtctg gaccgccttc gccaatgccg cgatcgccgt gtcgtcgaga
    35041 tgagcgcata agtcgcctgg gttttcgaaa acggcttggg caccggcctt ttcgagttcg
    35101 ccaccggaaa cgccgccgct gagcactgcg atcgtcggca cgccggctcg ctcgcaggcc
    35161 tcgacgtccc agatggtgtc gccgatgaag actgcgttgg ccgcgtccac gccggcgcgg
    35221 tcgagggcga cctggatgat gtccggccgg ggcttggcgg tgtcgacatc cttcgatgac
    35281 gtcaccacgg atatcaggtc gtcgctcgcc agcacgttgc gcagaatcgc cagttcgtcc
    35341 tcgggtgccg atgtcgccaa tacgatctgc aggccgagcc catcgacggc ttcgaggagc
    35401 tcacgcgttc cgggcaagcg ccgcaacagc ggtgccatct cccggtagta ctgcgaatgc
    35461 aaatccttca gccgcgagcg ggtgtcgtcg tcggcgcgtt cggccaacgt agccaccagc
    35521 gtggagccgt ccatcccgat cgaccggtga atccgccagg cctcgatgtc gatgccgagt
    35581 tcgcggaatg cccggtacca cgcgtggaca tgcagatagt tcgagtccac gagcgtgccg
    35641 tcgacgtcga acagcaccgc cgggcgcgcc gctgtcacaa ccggtgctgc ttgcgggtcg
    35701 gcgtgtcggc ctgcgcctgc cgggcttcgt ctgcgccttc gcgcctcgtg tcgtcgtgct
    35761 tcgggccttg gtcgtcgcgg cgcttggggt caagggcgtc ggcgcgctcg cgctgggcgt
    35821 cgagttcctc gcgcgaggtg gtaacaacct cgcgatgcga tgcggctctc tgttgcagcc
    35881 tggccgcctc cgctgctttg gcctcggcct cggcctgggc tgcacgcgcc ttcgcctggg
    35941 tttcgtcggc gaatgcctcg cgcttgtcga gtcgctgtgt ctgtcgccca acctcctcgc
    36001 gaatgtgctc ggcctgtgaa tgccgccgct tgttacgcgc gaggaacgcc aggagggcga
    36061 cgatgatgat cgcgacgacg accgcgacaa cgatccagat cacggtgttg gacatatcgg
    36121 ttccttccgg tttacgaacc cgctaattcc gtgggatgcc caacggcgcg atacctaaac
    36181 cgaatgcttt gccatgccgg gtgtcagggg tagcctgaca cgcaggtttg tcaggatgga
    36241 cctgacagcg gaggagggtg tcgatgtcga agatgacgtc cgaattgcgg tgctcgttct
    36301 gcggcaaacc gcacaccgac gtcgggaagc tcgttgcggg gccaggcgtc tacatctgcg
    36361 acggctgtat caacctatgc gtcgacgtca tcgccgaggc gacgcaggcc ggaccaccgc
    36421 cgtcgctgcc cgactgggcc agcttctccg atgacgaact gctgcagcgg attccgttca
    36481 tcgcggcatc ggttgccaac atcgaggccg aacttcgcga tcgcgtcgac gaactgcgcg
    36541 accgcggcgt gtcatgggcc cgcatcggtg gcgcgttggg catgacgcgc caatcggcgt
    36601 gggaaaggtt ctcccccgcg actacttgac gatcagcgtt ccgtgcatag aggggtgata
    36661 cgtgcagtgg aaagcgtatt cgcccggttc ggtcggcgcg gtgagcgtgc cctgttcctt
    36721 gccctccacc tcgacatcga acttgcctgc cgtatccgag gtcaccgaat gctcggcgga
    36781 gtcgtcgttc ttgatggtga tcgtggctcc aggcggcacc gtgatggctt cgccgaagct
    36841 catgttcgcg atcgtgatgg ttgccgcgct ggccgcggcc ggtccgctcg ttgcttccgc
    36901 ggtgcccgtg gctgtcgccg tcccggtctg cgtctcctgg gtggtgttgc tcgatccgcc
    36961 gcaggccgtg gcaaacgccg cgacgagaca cagggcggca cagaacaaac cgaatctctt
    37021 cattgtgacc cctctcgatt gaaccctcgg ctgagacacg attatcgatc cgaattggtt
    37081 caaacacggc caaattcggg gaatgagtga ggcgttgcgt tctacgtcaa aagtagccat
    37141 tggcaggcgg ccagacgccg ttgacggcgt aattgcctgc ggcataggcc ttgtaggcca
    37201 acggactatg ggttgccaca gtgcgggcgt ttcgccagtg ccggtcaagg ttgagtttgc
    37261 gggcggtggc cgacgctccc ccggtgtcga acaatcgctc cgccgccacg agcgtgagtc
    37321 gttccgccac cagctgaccc ttggcaactg tgaccgacaa ctcggccagc gcgtcgggat
    37381 cggcctgcag cgcattgtcg accaccgcgt cgatcgcgtc tgcggcggtg agcaccacgg
    37441 cttcggccgc ggcggcgttg gcggcgatgt cgccaacggc ctgaaggatg aacggatcac
    37501 ccgaagcggt atcggccagt gaatgcgaag ccgggcgcgc cttctcgcgg acgtatgcca
    37561 ccgcgtcatc gagaacgccg tgcgcgatgc ctgctgtcac tgcggccagg tacagctgca
    37621 ggaacgcggt cgagtggccg agatgtttgc cgtcggaaac ggtggttacc tcgaccgggt
    37681 gcacgacgac atcggtgaat cgggtgcctc cgctggccgt cgtccgttgg ccgaaaccgt
    37741 cccaatcgtc gtagagctcg acgcctgtgc ggtcggtcgg cacgatcgcc tgcagatccc
    37801 tgccctcggc gtcgatcgcg gaaacggcga tcctgtcggc gaacagcgtt ccggtggaat
    37861 agaacttgta gccgttgagc cgcagcaccc cgtcggtgtc ggcgagcagc gtggtgtccg
    37921 aactcgcagg ggccttgcca ttggtgtcgg tgaccgcgtt tccgaagacc agcccggagt
    37981 tgactgctgg aaaccagtgc gcccgttcgg
//
LOCUS       DASRJA010000265         5144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3348527,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000265 DASRJA010000000
VERSION     DASRJA010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3348527"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..490)
                     /locus_tag="VFC01_11920"
     CDS             complement(158..490)
                     /locus_tag="VFC01_11920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN80379.1"
                     /translation="MTTDKTGAPTTVTQESDRFSISVEGQKAGFTEYADRDGQRIFPH
                     TEVADEFEGRGLATILVNEALKTTRDSGLRIVAVCPLVAAFVKKHHEFDDVVDPFDTD
                     LKRWLANR"
     gene            complement(499..1464)
                     /locus_tag="VFC01_11925"
     CDS             complement(499..1464)
                     /locus_tag="VFC01_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZN80380.1"
                     /translation="MSNTDTAPSEVACLASPFAGVLHPREVPLGGPRAIKVRRTLPQR
                     ERSLIGAWCFADHYGPHDVRGGTGMNVPPHPHTGLQTVSWLFSGEVEHRDSAGVHALV
                     RPGELNLMTAGAGICHSEVSTADTTVLHGVQLWVALPDSDRDTARDFAHYVPEPRFVD
                     GATMRVFLGELEGDRSPVHTFTPLLGAQLDLEPGADLTLEVDPVFEHGVLLDQGDVEV
                     GGTALEVADLAFQAAGSDRLELVNRGHGPARVVLLGGPPFPEQLVMWWNFVGRSHEDI
                     VRYRQMWEEHDDRFGAVQGYQGAVSRLPAPPLPNATLRPRPNPQV"
     gene            1979..3577
                     /locus_tag="VFC01_11930"
     CDS             1979..3577
                     /locus_tag="VFC01_11930"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN80381.1"
                     /translation="MALAAATPPTATVTLPRILTGLLAAFGFGPQATNGPVAPAVPQP
                     TLLEVLAGAWREISRTFFNQGPTIAYDPGENSLVDGSIVGKLTPVDADSTNITFSSSK
                     PAHGDVTVDPDGTFTYTPDATYSGYDSFEVSVSDAGSGFHIHGLPGLLNLVTFGLLGD
                     SGHTHTQTVTIGDAPSGGYTVTTVVSGLNQPTDFRFLPDGRILIAEKGGAIKVSNEDG
                     QLQSTPLVTLPTKTVWARGLTAVEVDPDYEQNGYIYVSYIRDDNYQRLSRLTVTDPTA
                     EVLTVDPDSEVVLVQGTQLAGDDHHGGGLSFGPDGKLYWSTGDNVCCSVIDGSNSRNL
                     SNMYGKVLRLNPDGTAPDDNPFYDGDGPNYDAIYATGFRNPFRLTFTPDGQQLVVDVG
                     QSVWEEVNLVTAGADYGWPKAEGPCDGSGTTSCSTPSSYANPIYAYRHTAGGNSITAV
                     MAYTGTPSSSGQNTVLIADFTQGWVKELTFNSDYSALISERLFEDAAPGATNKLAQGP
                     DGSIYQLTFDGKLTRISPSSDVVV"
     gene            complement(3654..4073)
                     /locus_tag="VFC01_11935"
     CDS             complement(3654..4073)
                     /locus_tag="VFC01_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HZN80382.1"
                     /translation="MTNWINTVSRDHVERGVRGRFTQANHGKPHMLKKMARGDWIVFY
                     SPKTVYPDGEPLQAFTAIGQVVDDEPYQVEMSPDFQPWRRNVDFLECAETPIRPLIDQ
                     LDFVEDKARWGYKFRFGVFKIDDHDLDVIRSAMTASD"
     gene            4146..>5144
                     /locus_tag="VFC01_11940"
     CDS             4146..>5144
                     /locus_tag="VFC01_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZN80383.1"
                     /translation="MSDAGPDGVRADHAELLRRRALTEDAARPDAVQRRHAANARTAR
                     ENIEDLVDAGSFVEYGRFAIAAQRFRRELADLIARTPADGIVAGTARVNGSACAVLSY
                     DYTVLAGTQGALGHRKKDRLFELIERMRLPTVFFAEGGGGRPGDTDYPVVSALDARAF
                     KLWAALSGVVPRIAIVKGRCFAGNAVIAGCADLIVATEDASIGMGGPAMIAGGGLGDV
                     HPDAVGPVSMQQPNGVLDVVVADEAAAVAVTKRLLGYFQGTTKPGPTADQTRLRTMIP
                     ERERRAYKAAPIIETLADEASVTFLREKFAPEMVTALARIEGRAVGVIANNTMVMAG"
BASE COUNT          940 a         1671 c         1648 g          885 t
ORIGIN      
        1 gctgatcgtc tcaccccacg ggccgcgagc gatgggttgc ggtacgaagg cgtcgccgtc
       61 ggcaacgaag aatgccgacg ggccgctgcg gggagccggt gcgtcgatcg cggggtcctg
      121 ggtggtcatc gtgatcgacg atatcgggct agctcatcta acgattagcc agccagcgtt
      181 tgaggtcggt atcgaacggg tcgacgacgt cgtcgaactc gtgatgcttc ttgacgaagg
      241 ccgcgaccaa cgggcacacc gcgacgatgc gcagcccgct gtcgcgggtg gtcttcagcg
      301 cttcattcac gagaatcgtt gccagaccgc ggccctcgaa ctcgtcggcc acctccgtgt
      361 gcggaaagat ccgttggccg tcgcgatcgg cgtactcggt gaagccggcc ttctgcccct
      421 cgacggagat gctgaagcgg tcggattcct gggtgacggt cgtcggtgcg ccggtcttgt
      481 cggtggtcat gacctcttct atacctgggg attgggtcgc gggcgcaacg tcgcatttgg
      541 cagcggcggg gcaggcagcc gcgacaccgc tccttgatat ccctgcaccg cgccgaagcg
      601 gtcgtcgtgc tcttcccaca tctgccggta acgcacgatg tcctcgtggc tgcggccgac
      661 gaagttccac cacatcacca gctgctcggg aaacggcggc ccgccgagca gcacgacgcg
      721 cgcgggcccg tggccacggt tcacgagctc tagccggtcg cttccggcgg cctggaaggc
      781 cagatcggcg acctcaagcg ccgtcccccc gacctcgacg tcgccctggt cgagcagcac
      841 gccgtgctcg aacacgggat cgacctcgag ggtgaggtcg gcacccggtt cgaggtcgag
      901 ctgcgcgccc agcagcggag tgaaggtgtg cacgggggag cggtcgcctt cgagctcgcc
      961 gaggaacacc cgcatggtgg cgccgtcgac gaagcgcggc tccggcacgt agtgggcgaa
     1021 gtcgcgtgcg gtgtcgcggt cggaatcggg cagcgccacc cacagctgca caccgtgcag
     1081 cacggtggtg tccgcggtag agacctcgga gtggcagatc cccgcgcccg ccgtcatcag
     1141 gttcagctcg ccgggccgca ccagcgcgtg caccccggcg ctgtcgcgat gttccacctc
     1201 accgctgaat agccaactga cagtttgcaa tccggtgtgt ggatgtggcg gcacgttcat
     1261 tcccgtgccg ccacgcacgt cgtggggacc gtagtgatcg gcgaagcacc acgccccgat
     1321 cagcgaccgc tcccgctgcg gcagcgtgcg tcgcaccttg atcgcgcgcg gcccacccag
     1381 cggcacctcg cgcggatgca gcacgcccgc gaatggcgaa gccagacagg cgacttcgga
     1441 cggcgccgta tcggtgttgc tcacgaactc acggtagtcc ccggtgccgg tggctactga
     1501 cagtcagcta gctgtagcgt ccactgaatc agccgctgag gaacgcgtat taccagaata
     1561 actatttggg agccttaggt cctcggaggg acgtcgccag cgaacaatgg taactatgct
     1621 tgcgagataa tgtttagcaa acacaagtaa cgcacgaaga tcggtggtca tgttggcttg
     1681 acggaccggt gcgcgcagca gtccaacgca atcgacgcgc gtgtgtgacg tgaatgtcgg
     1741 tggcgcccaa ggcccttgcg tatcggcggt gctattagcg gttcggatgc gacgagcgga
     1801 tcgcagtaac cggtgaggta tcagggactc gcaagcgaat ttaacaggac cgtagtccga
     1861 ttcgctagaa gtcaacgccg acgaggctca ggcgatcgaa tcagatttgg tgcgcacgag
     1921 ggcgacgcag gcgttctcaa ctgtggcaac tcgatcgacg gctgacagtg agccggtgat
     1981 ggccctggcc gcggcgacac caccgacggc gaccgtgacc cttccccgca tcctcaccgg
     2041 gctgctggct gccttcgggt tcggtccgca ggccacgaac ggcccagtgg ccccggctgt
     2101 accccaaccg acgctgcttg aagtgctggc aggggcgtgg cgcgagatca gccggacctt
     2161 cttcaaccaa ggccccacca tcgcctatga cccgggcgaa aacagtctcg tcgacggcag
     2221 catcgtgggg aagctgaccc ccgtcgacgc cgacagcacc aacatcacct ttagctccag
     2281 caagcctgca catggcgacg taacggtcga tcccgacggc acgttcacct acacgcccga
     2341 cgcgacgtac agcggctacg actccttcga ggtcagcgtc agcgacgccg gcagcggatt
     2401 ccacatccac ggcctgcccg gtctgctcaa tctcgtaacg ttcggcctac tcggcgatag
     2461 cggccacacc catacccaga ccgtcaccat cggcgacgcg ccatcgggtg gttacaccgt
     2521 caccactgtc gtgtcgggtc tcaaccaacc gacggacttt cggttcctgc cagacggtcg
     2581 aatcctcatc gctgaaaagg gcggagcgat caaggtatcc aacgaggatg gccagctgca
     2641 gagcacgccg cttgtcacgc tgcccaccaa gacggtttgg gcgcgcgggc tgaccgctgt
     2701 tgaggtcgat ccggactatg agcagaacgg ctatatctat gtgtcgtata tcagggacga
     2761 caattaccaa cgtttgtcgc gcctaacagt tactgatccg acggccgagg tcctgacggt
     2821 agacccagac tctgaggtcg tacttgtgca aggcacgcag ctggccggcg acgaccatca
     2881 cggcggtggg ctcagtttcg gtcctgacgg caagctctac tggtccacgg gcgacaacgt
     2941 ctgctgcagc gtgatcgacg gcagcaactc acggaacctt tcgaacatgt acggcaaggt
     3001 tctgcgcctt aacccggacg gcaccgcacc cgacgacaac ccgttctatg acggcgatgg
     3061 tcccaactac gacgccatct atgccaccgg cttccgcaac ccgttccggt tgacgtttac
     3121 gccggacggc caacagttgg tcgtcgatgt cggccagtcc gtgtgggaag aggtgaacct
     3181 cgtgacggcc ggcgccgact acggctggcc gaaggccgaa ggtccctgcg acggtagcgg
     3241 cacaacgagc tgttcgacac cgtcctcgta tgccaacccg atctatgcct accgccatac
     3301 cgccggcggc aattcgatca cggctgtcat ggcctatacg ggcacgcctt ccagcagtgg
     3361 ccaaaacact gtcctgatag ctgatttcac tcagggctgg gtcaaggaat tgacgttcaa
     3421 ttcggattac tcggcgctga tcagcgagag gctgtttgaa gatgcagcgc ccggcgccac
     3481 aaacaagctg gcccagggac cggacggcag catctatcaa ctgacgttcg acggcaagct
     3541 cacccgcatt tccccgtcga gcgatgtggt cgtatgacgg cgcggcgacc tcacgcggat
     3601 ggagtgtgct gggcgaatag cgaagccgca caagagactt cgatgcgcag gcattagtcg
     3661 gaggcggtca tagccgagcg gatcacgtcc aggtcgtgat cgtcgatctt gaacacgccg
     3721 aatcggaact tgtagcccca gcgcgctttg tcttcgacaa aatcgagttg atcgatcagc
     3781 ggccgtatcg gtgtctcggc gcactccagg aaatcgacgt tgcggcgcca tggctggaaa
     3841 tccggtgaca tctcgacttg gtacggctcg tcgtcgacca cctgtccgat ggcggtgaag
     3901 gcctgcaacg gctcgccgtc cggatagacg gtcttcggcg aatagaagac gatccagtca
     3961 cccctggcca tcttcttcaa catgtgcggc ttgccgtggt tggcctgtgt gaagcggccc
     4021 cgcacgccac gctcgacgtg gtcacggctg acggtattga tccagttggt catgtccgca
     4081 cggtaagccg ggttgccgac agattgccgt cgcccatctg cggcctgtgg ataacatcgg
     4141 accccatgag cgacgcaggg cccgacggcg tccgcgccga tcatgcggag ttgttgaggc
     4201 gtcgcgcgct caccgaggac gccgcgcgtc ccgacgcggt ccaacgccgg cacgcagcca
     4261 acgctcgcac cgcgcgcgag aacatcgagg atcttgtcga cgcgggctca ttcgtcgagt
     4321 acggccggtt cgcgatcgcc gcccagcgat ttcgccgcga actcgccgac ctgatcgcgc
     4381 gcacccccgc cgacgggatc gtcgcgggca ccgcgcgcgt caacggcagc gcgtgcgccg
     4441 tgttgtccta cgactacacc gtgctcgccg gcacccaggg cgcgctcggc catcgcaaga
     4501 aggaccgcct gttcgaactc atcgagcgga tgcgattgcc caccgtgttc ttcgccgagg
     4561 gtggcggcgg acgacccggc gataccgact atcccgtcgt gtccgcgctc gacgctcgcg
     4621 cgttcaagct gtgggcggca ctttcgggtg tggtaccgcg gatcgcgatc gtcaagggcc
     4681 gctgctttgc cggcaacgcg gtgatcgctg ggtgcgccga tctgatcgtc gcgaccgaag
     4741 acgcgtcgat cgggatgggt ggaccggcga tgatcgccgg aggaggcctc ggcgacgtcc
     4801 atcccgacgc ggtgggaccg gtctcgatgc agcagcccaa cggtgtgctc gacgtggtcg
     4861 tcgccgacga ggccgcggcc gtcgcggtga cgaagcggct actcggctac ttccagggca
     4921 cgacgaaacc cggcccgaca gccgaccaaa cacgcctgcg cacaatgatt cccgagcgcg
     4981 agcggcgcgc ctacaaagcc gccccgatca ttgagacgtt ggccgatgaa gcgtcggtga
     5041 cgttccttcg cgagaagttc gcaccagaga tggtcaccgc gctggcccga atcgaaggcc
     5101 gcgccgtggg cgtcatcgcc aacaacacga tggtgatggc cggc
//
LOCUS       DASRJA010000266         6148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_51151,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000266 DASRJA010000000
VERSION     DASRJA010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51151"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFC01_11945"
     CDS             <1..297
                     /locus_tag="VFC01_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80384.1"
                     /translation="IDKFMLVAPSADIEYAVVGPPKTFAEAVRGGSTQSPAFRVGFHC
                     SPGRRRKPASKLYSGLRGSDGTRVDGLVMGYWDRPFDEAVDLMNEWRTRYRAAG"
     gene            316..759
                     /locus_tag="VFC01_11950"
     CDS             316..759
                     /locus_tag="VFC01_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN80385.1"
                     /translation="MITECGIDIDAPAAVVWDVFSDVERWPEWTASVTRLVGLDGPAL
                     EVGKRFEIKQPRMPKLVWEVTELTPGASWTWVQRSPGGLTVARHDVIAESDRRTQVRQ
                     QLDQRGPVGAAVGLLMRGMTRRYLDLEAKGLKARSEQRRVDGPTT"
     gene            743..1276
                     /locus_tag="VFC01_11955"
     CDS             743..1276
                     /locus_tag="VFC01_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN80386.1"
                     /translation="MARPPDRERRQELLDTVVKECAERGIGDRSLRDVAAAVGTSHRM
                     LLHHFGSRDELLLAIVGEIERRQRGLLSELPTEPGAAIAAMWADLRRPELRPFERLFF
                     ECYARGVQGEQPFARMLPEAVEAWLADDGSTDPALMRLGLAVMRGLLLDLVATDDRTG
                     VDAAAQAFIDLFRRAVG"
     gene            1385..1747
                     /locus_tag="VFC01_11960"
     CDS             1385..1747
                     /locus_tag="VFC01_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80387.1"
                     /translation="MKLKRTAAKATLVGAMSIAVAGFGAGLAHADPPFPPPPWPAPAL
                     DDGPGANMGAPGNPAPPGLDYASPPGHGGPISEDAVVPMWAPPAPPPPFWAPWLPVVW
                     NAEFNAWGVWWNGSFQTL"
     gene            complement(1804..4236)
                     /gene="ptsP"
                     /locus_tag="VFC01_11965"
     CDS             complement(1804..4236)
                     /gene="ptsP"
                     /locus_tag="VFC01_11965"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HZN80388.1"
                     /translation="MTVGLVVVSHSRALARAAVALAQEMLHGKQIRITIAAGLDDTTF
                     GTDAAQIVDAVTAADQGGGVVVLMDLGSAVLSAELALELLDDDVRERVVLCPAPLVEG
                     LVVAAVAAASGASREEVAAEAAGALAGKIGHLGTAPAVTAAEATAEADELTGSFVVAN
                     PHGLHARPAARLVQEVRRRDAHARIRNRRAESEWVDAGSLSKIATLGVRSGDEVEVRV
                     SGTQAAETLDHILSLAARNFDESTVDGAPAPEPTVHRPIGASPGLGIGPAQSTRMGAI
                     TIPDTPAEEPAVEWRRLGKAVASVRRTISQLRIRTARDVGEAEAAIFDAHQLLLDDAA
                     LLDDVRGRIDSGQSAASAWSVAVQNLAAEFAAVTDPYLQARADDVRAVGDQVLRAMLG
                     GSESSEGPTGVVIAADLSPAEAAELNPARVAAVLLAFGSPHAHNVILLRAKGIPVIVG
                     AGTAVLSIPDGTVLAVDGGRGEFIVDPAEDVSQEFAARVATLAQRQRQAHARAAEPAV
                     TTDGITVAVGANVGSVDDARAAAAQGADLAGLVRTEFLFLGRQDAPDVEEQFAVYRKI
                     AESLQGRRITLRTLDVGGDKPLEFLPSPAEMNPYLGVRGIRLSLAHPELLADQLLAMV
                     RLAQQTPVSVMFPMITTLDELFAARELLETTIRRAGGSRPDGLQVGMMVEVPAAALKA
                     AAFARHVDFLSIGTNDLTQYTLAADRNNDAVAAIGDTFDPGLLQLIRATCQGAAGKAS
                     VSVCGEFAADECATSLLLGLGVDALSVTPPAIPATKEAVRAVNTFDAQRIAADALAAD
                     SAASVRARLNRD"
     gene            complement(4241..4873)
                     /gene="dhaL"
                     /locus_tag="VFC01_11970"
     CDS             complement(4241..4873)
                     /gene="dhaL"
                     /locus_tag="VFC01_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HZN80389.1"
                     /translation="MDLAQLTAWIREYARVIGENAQHLTDLDAAIGDADHGINMERGM
                     SAVLGALDEAAPADMSALCKQVGMTLVKSVGGASGPLYGTFFLRMAGALGTGDRVDAA
                     DFAKALRAGVEGVVQRGRAEAGDKTMFDALAPALDALDAALSSGGDLAVALADATVAA
                     EKGRDATESMVARKGRASYLGQRSVDHVDPGATSAAMLIAAAASAFATGG"
     gene            complement(4874..5872)
                     /gene="dhaK"
                     /locus_tag="VFC01_11975"
     CDS             complement(4874..5872)
                     /gene="dhaK"
                     /locus_tag="VFC01_11975"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HZN80390.1"
                     /translation="MKKLINDPADVISEALRGMSVAHPELRIDHTNRIVYRSDAPIRG
                     KVGLISGGGSGHEPLHGGFVGQGMLDAACAGEVFTSPVPDQMLEATKNVDGGAGVLHI
                     VKNYTGDVMNFEMAAELADAEGVTVESVVVDDDVAVQDSTYTAGRRGVGATVLVEKIA
                     GAAADQGRTLSEVADVARRVNAASRSMGVALTSCTVPAVGHPTFDLSDTEIEVGIGIH
                     GEPGRQRMPMQPARAVAELMLDPVLADLDFSGDDGVILFVNSMGGTPLIELYVMYSEF
                     AAILDKAGVRIARSLVGPYITSLDMAGCSVTLLRADDELLRLWDHPVNTPALRRGC"
     gene            complement(5885..>6148)
                     /locus_tag="VFC01_11980"
     CDS             complement(5885..>6148)
                     /locus_tag="VFC01_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HZN80391.1"
                     /translation="IGLVVFAAIIPVAPTTGASINPARTFGPMLVQQIAGGSVSWSQL
                     PVYLAAELLAGALAALAYVAISRTRADATPAPVAVPTAQTTAA"
BASE COUNT          969 a         2093 c         2108 g          978 t
ORIGIN      
        1 atcgacaagt tcatgcttgt cgcgccgtca gccgacatcg agtacgcggt ggtaggcccc
       61 ccgaagacgt tcgccgaggc cgtgaggggc ggcagcaccc agtcgccggc attccgcgtc
      121 ggctttcact gctcgcccgg ccgtcgccgg aagcccgcga gcaagctgta cagcggcctg
      181 cgcggaagtg atgggacgag ggtcgacggt ctcgttatgg gctactggga ccgtcccttc
      241 gacgaggcgg tcgatctgat gaacgagtgg cgcacacgct accgggcagc cgggtaatca
      301 tgtaccatct ggtacatgat tactgagtgc ggcattgata tcgatgcgcc tgccgctgtc
      361 gtctgggatg tgttcagcga cgtcgagcgc tggcctgaat ggaccgcgtc ggttacccgg
      421 ctcgtcggtc tcgacgggcc cgctctggag gtcggcaagc ggttcgaaat caagcagccg
      481 cgcatgccga agctggtgtg ggaagtcacc gagttgacgc cgggtgcctc gtggacctgg
      541 gtgcagcgtt cgcccggtgg cctgaccgtc gcccgccacg acgtcatcgc ggagtccgac
      601 cggcgcactc aagttcggca gcaactcgac cagcgcgggc cggtcggcgc cgcggtgggt
      661 ctgctgatgc gcgggatgac aaggcgctac ctcgatttgg aggcgaaggg tctcaaggcc
      721 agaagcgagc agcgacgcgt cgatggcccg accacctgac cgcgaacggc gccaggagct
      781 gctcgacacg gtggtcaaag agtgcgcgga acgcggcatc ggtgaccgat cccttcgcga
      841 cgtcgcagcg gccgtcggca ccagccaccg catgctgctg caccacttcg gctcgcgcga
      901 cgagctcctg ctggcgatcg tcggagaaat cgagcgccgc cagcgaggcc tgttgtccga
      961 gctgccgacc gagcccggcg cagcgatcgc ggcgatgtgg gccgaccttc gccggccgga
     1021 gctgcgcccg ttcgagcgcc tcttcttcga gtgctacgcg cgcggcgtac agggggagca
     1081 gccgttcgcc cggatgctgc cagaggccgt cgaggcgtgg ctggcagatg acgggtcgac
     1141 cgacccggcg ttgatgcgcc tcgggctggc ggtcatgcgt ggcctgctcc tcgacctcgt
     1201 ggccaccgac gatcgcaccg gcgtcgatgc ggcggctcag gcgttcatcg acctctttcg
     1261 gcgggcagtc ggttagcggg gtttagcccg cggggacggc tgggtagccc taagcgctcg
     1321 gggggttcag tcctgaccag ctcgtccgcg cacgtcgtgg acgcgccgat cgaaggagta
     1381 atgaatgaag ctcaagcgaa ccgcggccaa agcaactctg gtgggtgcaa tgagcatcgc
     1441 cgtggcgggg ttcggcgcag ggttagcgca cgctgatccg ccgttcccgc cgccaccgtg
     1501 gccggccccg gcacttgacg atggccctgg tgcgaacatg ggcgcgccgg gcaatccggc
     1561 gcccccggga ctcgactacg cgtcgccgcc gggacatggc ggaccgatat ctgaggacgc
     1621 ggtcgtaccc atgtgggccc cgccggctcc gccgccgccg ttctgggcgc cgtggctgcc
     1681 ggtcgtgtgg aatgcggagt tcaacgcgtg gggtgtctgg tggaacggta gcttccagac
     1741 gctgtaacac atctccacgg gacggtcgcc ctgccataga agcagggcga ccgttctgtg
     1801 cgctcagtcc cggttgagcc gggcccgcac ggatgctgcg ctgtcggccg caagcgcgtc
     1861 cgccgcaatc cgttgcgcgt caaaggtgtt caccgcacgg acggcttcct tggtggccgg
     1921 aatggcgggc ggcgtgacag acaacgcgtc cacgcccagc ccgagcagca gcgaagtggc
     1981 acactcgtcg gccgcgaact cgccgcacac cgacaccgac gcttttccgg cggccccctg
     2041 acaggttgcc cgaatcagtt gcagcagacc ggggtcgaac gtgtcgccga tcgccgcgac
     2101 ggcgtcgttg ttgcggtcgg cggcaagcgt gtactgcgtc agatcgttcg tgccgatcga
     2161 caagaagtcg acgtgccgag cgaatgcagc ggccttgagc gcggccgcag ggacctcgac
     2221 catcatgccc acctgcaggc cgtcgggacg actaccgcca gcgcgcctga tcgttgtctc
     2281 taaaagctca cgggccgcga acaattcgtc gagcgtggtg atcatgggga acatcacact
     2341 gaccggtgtc tgctgggcca gccgcaccat cgccaacaac tgatctgcca gcagctccgg
     2401 gtgcgccaac gacaggcgga tgccacgaac acccaggtat gggttcatct cggcgggcga
     2461 cggcaggaac tccaatggct tgtcgccgcc gacatccagg gtgcgcaacg tgattcgccg
     2521 tccttggagc gactcggcga tcttgcggta cacggcgaac tgttcctcga cgtccggcgc
     2581 gtcctggcgg ccgaggaaga gaaactctgt gcggacgagg cccgcgagat cagccccctg
     2641 tgccgcggcc gcgcgggcgt cgtccactga gccgacgttc gcgccgaccg caaccgtgat
     2701 gccgtcagta gtgacagctg gctcggcggc tcgggcatgt gcctggcgct gtcgttgggc
     2761 gagggtggcg acccgggccg caaactcctg actgacgtcc tcggcagggt caacgatgaa
     2821 ctcgccacgc ccgccgtcca ccgcgaggac cgtgccgtcc ggaattgaca gcaccgccgt
     2881 acccgcaccg acgatcaccg gaattccttt tgcccgcagc aggataacgt tgtgtgcgtg
     2941 cgggctgccg aacgccagca gcaccgccgc gacccgcgcc ggattgagct cggcggcctc
     3001 cgcggggctc aggtccgccg caatgaccac acccgtcggc ccctccgagc tttcgctacc
     3061 gcccagcatc gcccgcagca cctgatcgcc gacggcgcga acgtcgtcgg cgcgggcctg
     3121 caggtacgga tccgtaacgg cggcgaattc ggcagccagg ttctgcacgg caaccgacca
     3181 cgcggacgcc gcggactgac cggagtcgat gcggccgcgc acgtcgtcga gcaatgccgc
     3241 gtcgtcgagc agcagttgat gcgcgtcgaa gatagccgcc tcggcctcgc cgacgtcccg
     3301 ggcggtgcgg atgcgtagtt ggctgatggt gcggcgtacc gatgcgaccg ccttgccgag
     3361 ccggcgccac tcgaccgcgg gttcctctgc gggagtgtcg gggatcgtta tggcacccat
     3421 gcgagtcgac tgcgcaggcc cgatgcccaa cccggggctg gcgccgatcg ggcggtgcac
     3481 tgtcggctcg ggggcagggg cgccatccac cgtcgactcg tcgaagttgc gggcggccag
     3541 cgacaggatg tggtcgaggg tctcggcggc ctgcgtaccc gagacccgca cctcgacctc
     3601 gtcaccggat cgcacaccga gcgtcgcgat cttcgaaaga ctgcccgcat caacccattc
     3661 cgactctgcg cggcgattcc ggattcgggc gtgcgcatcg cggcggcgca cctcctgcac
     3721 cagtcgcgcg gccgggcgcg catgaagtcc gtgcgggttc gcgacgacga agctgccggt
     3781 cagctcgtcg gcctcggctg ttgcctcagc tgccgtcacc gcaggcgccg tcccgaggtg
     3841 accgatcttt cctgccagcg cacccgcagc ctcggcggcg acttcctcgc gtgaggctcc
     3901 gctcgctgcg gccaccgcgg cgaccaccaa tccctcaacg aggggagcgg ggcacaacac
     3961 cacgcgttcg cggacgtcgt cgtccagcaa ttccagtgca agctctgccg agagcacggc
     4021 gctgccgaga tccatcagca ccactacacc cccaccctga tcggcagcgg tgacggcgtc
     4081 gacgatctgc gcggcgtcgg ttccgaaggt ggtgtcatcg aggcctgccg cgatcgtgat
     4141 acggatctgc ttgccgtgca acatctcctg ggccagcgcc acagcggcgc gcgccagtgc
     4201 ccggctgtgc gaaacgacga cgagtccgac ggtcaccgcg tcacccacct gtggcgaacg
     4261 ccgacgccgc ggccgcaatc agcatcgccg ccgaggtcgc gccgggatcg acatggtcga
     4321 cactgcgctg tccaaggtag ctggcacggc ctttgcgggc gaccatggat tccgttgcat
     4381 cgcggccctt ttcggccgcg acagtggcgt cggccagtgc caccgcgagg tcgccgccgg
     4441 atgacagcgc ggcatcgagt gcatcgagcg cgggtgccaa cgcgtcgaac atcgtcttgt
     4501 cgccggcttc ggcgcgaccg cgctgcacca cgccctcgac gccggcgcgc aaggctttgg
     4561 cgaagtccgc cgcgtcgacg cggtcgcctg tgcccaatgc gcctgccatc cgcaagaaga
     4621 acgtgccgta cagcgggccg ctggccccgc cgaccgactt caccagcgtc atacccacct
     4681 gcttgcacag cgcggacata tcggcagggg ccgcttcgtc cagcgcgccg agcaccgccg
     4741 acatgccccg ctccatgttg atgccgtggt cggcgtcgcc gatcgccgcg tccaaatcgg
     4801 tgagatgttg ggcgttttca ccgatcacgc gcgcgtattc acggatccat gcggtgagct
     4861 gggcgagatc cattcagcag cccctccgca gtgccggcgt gttgaccggg tggtcccaga
     4921 gccgcagcag ctcgtcatcg gcgcgtagca gcgtgactga gcagccggcc atgtccaggc
     4981 tggtgatgta cgggcccacc agcgagcgcg cgatccgaac gccggccttg tcgaggatcg
     5041 cagcgaattc cgaatacatg acgtacagct cgatcagcgg ggtgccgccc atactgttga
     5101 caaagagaat tacgccgtca tcgcccgaga agtcgaggtc agccagcact gggtcgagca
     5161 tgagttcggc aaccgcgcgc gcaggctgca tgggcatccg ctgccgtccg ggttcgccgt
     5221 gaatgcctat gccgacctcg atttcggtgt cggacaggtc gaacgtcgga tggccgactg
     5281 ccggcaccgt gcacgatgtc agcgcgacac ccatgctgcg cgatgcggcg ttgacgcggc
     5341 gcgcgacatc cgccacctcg gagagggtgc ggccctgatc cgccgcggcg cctgcgatct
     5401 tctccaccag caccgtggct ccgacaccgc gccggccggc ggtgtaggta ctgtcctgca
     5461 cggcgacgtc gtcgtcgacg acgaccgatt cgacggtgac gccctcggcg tcggccagtt
     5521 cggcggccat ctcgaagttc atcacgtcgc cggtgtagtt cttcacgatg tggagcacac
     5581 cggcaccgcc gtcgacgttc ttggtggcct cgagcatctg gtcgggtacg ggggaggtga
     5641 aaacctcgcc ggcacatgcg gcgtcgagca tgccctgccc gacgaacccg ccgtgcaggg
     5701 gctcgtgccc ggagccgccg ccggagatca gtcctacctt gcctctgatc ggcgcgtcac
     5761 tgcggtagac gatccggttg gtgtggtcga tgcgcagttc gggatgcgcg acggacatgc
     5821 cgcgcaacgc ctcgctgatg acgtcggcgg gatcgttgat gagcttcttc atttccgggg
     5881 ctcctcaggc tgcagtggtc tgggcggtcg ggacggcgac gggcgccggc gtcgcgtcgg
     5941 cgcgcgtgcg cgagatcgcg acataggcca gggccgcgag agcgccggcg aggagttcgg
     6001 cggccagata caccggcaac tgactccacg acaccgaacc gccggcgatc tgctggacga
     6061 gcatcgggcc gaacgtgcgg gccgggttga tcgacgcgcc cgtggtcggt gcaaccggga
     6121 tgatcgctgc gaacaccacc aagccgat
//
LOCUS       DASRJA010000267         5855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1177598,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000267 DASRJA010000000
VERSION     DASRJA010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177598"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VFC01_11985"
     CDS             <1..1299
                     /locus_tag="VFC01_11985"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: protein motif:HMM:NF004829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HZN80392.1"
                     /translation="RVLAELGRSPQLSAAHGFPQMNMFDQPELEAMLRVNLKHYEQVT
                     VRGNVEVTSVTQTRPGRVRVSYLDRVRGGETAVEASYVLGCDGANSPTRAAIGAHMYG
                     LPFTQSWVVIDVDTDAELDQWEGCHQLCNPQRAGTYMRVGQTRYRWEFQLLDGESAAN
                     YQTIADIEPLIRPWLGDNPADAMKLVRVTSYTFRAQVASRWRDGNVFLLGDAAHLTPP
                     FVGQGMAAGFRDSMNLTWKLVGVLNKTLPESVLDTYEQERKLHAAAMILMAVSVGAAM
                     TGGGRLGDLIRNVVFPKMKDLRLPGTRASAAEGITPGLRRSALVIKSRAGGGLAGSMC
                     PNPVLGDGKRFDEVVGNRFALITSASLNPGVGDDVVRRGAVIIQAAPGSELDSWLRKG
                     RASAAIVRPDRVVMQAGRDAEAICAAVPTFCAGKPEKTEG"
     gene            1305..2201
                     /locus_tag="VFC01_11990"
     CDS             1305..2201
                     /locus_tag="VFC01_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80393.1"
                     /translation="MTELLVLNAGKGEMQAVSPDGTRVRTLIAGLDELPDGIVVDQRR
                     GHVYWTNMGAPDPGSGMPGDAAYTRNGSLERADLDGGNRVTIVPRSAFTTGKQLTADF
                     DDGTLYWCDREGMRVLSCKLDGSGLRTLVVSGVGAEAARHARNHPVGIAIDVDRRLLY
                     WTQKGAPNGGEGRIFRGPLDPAVGVSTPYGDGVELLWKDLPEPIDLHLVDDGATLLWT
                     DRGDPEGNTLNRAIVQPTVEAFEVLSTAYREAIGVAAVSETEFYVSDLGGSVRHIDLA
                     SGTDIELVNLGSPLTGIALAEL"
     gene            2324..2914
                     /locus_tag="VFC01_11995"
     CDS             2324..2914
                     /locus_tag="VFC01_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80394.1"
                     /translation="MTEWFDIWRNLTPGQGTLFGGAFVLCAGILTFGTGSLDRKSRAK
                     RLFYEEMKALYAQALRIGRDLELLKAVPPDGREKIVTEKIDAMQKVISELALTGNYQT
                     ADLAIAYTYQQSVQLAVWSREVEHDIGLPDQVRRWLDNMTDEELAALKEYGNVRIDRR
                     DVVEALRAELALYVPRWSRYRRVMRRPLHVDRYVLN"
     gene            complement(2993..>5855)
                     /locus_tag="VFC01_12000"
     CDS             complement(2993..>5855)
                     /locus_tag="VFC01_12000"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZN80395.1"
                     /translation="DLLRTVRQLDAAAPTRPSARASQAYVSIVEAIEAAAREQVDVAA
                     LLASEPADALAASSATRWSGLEAAQFDVFRDNATGPLAADLEAVLFSPSGITVTGVRD
                     ALANRTLDALREPSLAGWVEASGTRIASLRDLQDGARNNLAATASNDLAAARTRSLRD
                     VGISLAVLLIVTGLALALRRSITSPLREVSEAAQHLSSGDLTVQVGYRGRDEIGDVAT
                     AFRNLHVTVERLAVEIREMTAAVRDNRLEHRANIAAFEGTWSQLLTGLNDTMAAFAEL
                     EGRRERAERELSDFFQLSVDLLCIGNFDGYFTRVNPAFERTLGYTFAELTSRPFVEFV
                     HPDDRELTADAFASQTSGAELIGFENRYICSDGSLRWLQWTARAVPEEGFACAVARDI
                     TEQRRGQEEQAALRRVATLVAQGASPTETFSAVAAEVGQLFGGDVAAVLRFAPDREAT
                     LVGGWSIPGIEIPIGSRFNVAGTGVVVQVHDTRRPARIDRFEGPPGSIAAFFADCGAR
                     CGVGAPITVEGGLWGVLAIVSTDPERLPAGSELATAQFTDLVATAVANAEAREALRRV
                     ADEQAALRRVATQVAAAAPPNAVFAGVAEEVGRLLSVDRAYVARYDADDAATLVAAWS
                     ATGEALPLGTTTQIGGSMSAGTVSARVRETGRPVRVDRYEGDAGSAAFQLGVRTAVGA
                     PITVKSRPWGLIVVASTTDEPPPPETEARLGDFTELVAIAIANADAQAELTASRARIV
                     AAGDQARQRFERDLHDGTQQRLVSLVLELRMAQAVVPSELTELSAQVDRAVTAASDLL
                     DELREIARGIHPAILTKGGLTAALPALTRRCPIPVHLDMRIPRPLPEHVEASAYYIVA
                     EALTNVAKHSRASTVSVAAITDTADGVLHIAVSDDGVGDAHFAAGTGLVGVKDRVEAI
                     GGRIFLDSPLGAGTSLRVELPLAAPPLALEKPAGQADSG"
BASE COUNT          992 a         1912 c         1997 g          954 t
ORIGIN      
        1 cgggtgctgg ccgagctcgg gcgcagcccg cagctcagcg ccgcacacgg cttcccgcag
       61 atgaacatgt tcgaccagcc ggagctggaa gcgatgttgc gggtgaacct caagcactac
      121 gagcaggtca ctgtccgggg caacgtcgaa gtcacctcag tcacccagac ccggccgggt
      181 cgggtgcgcg tgagctacct cgaccgcgtc cgtggcgggg agaccgcggt ggaggccagc
      241 tacgtgctgg gctgcgacgg cgccaacagc cccacccgcg cggccatcgg tgcgcacatg
      301 tacgggctgc ctttcaccca gagctgggta gtcatcgacg ttgacaccga cgccgaacta
      361 gaccagtggg aaggctgcca tcagctgtgc aacccacagc gcgcagggac ctacatgcga
      421 gtgggtcaaa cccgctaccg ctgggaattc cagttgctcg acggggagag cgcggccaac
      481 taccaaacga tcgccgacat cgagccgttg atcaggccgt ggctgggcga caacccggcc
      541 gatgcgatga agctggtgcg ggttacctcg tacaccttcc gcgcgcaggt ggccagccgc
      601 tggcgtgatg gcaacgtgtt cctgcttggc gatgccgccc acctgacccc gccgttcgtc
      661 gggcagggca tggcggcggg cttccgggac tcgatgaacc tcacctggaa gctggtcggc
      721 gtgctgaaca agacgctgcc ggaaagtgtg ctggacacct acgagcagga gcgcaaattg
      781 cacgccgcgg ccatgattct gatggccgtg tcggtgggcg cagcgatgac cggcggtggt
      841 cgcctcggtg acctcatccg caacgtcgtg ttccccaaga tgaaggacct gcgcctaccc
      901 ggtacccggg ccagcgccgc cgagggcatc acccccgggc tgcgtcgatc ggcgttggtg
      961 atcaagtctc gcgctggggg cgggttggcc ggcagcatgt gccccaaccc ggtgctgggc
     1021 gacgggaagc gcttcgacga ggtggtcggg aaccgcttcg cgttgatcac ctcggcgtca
     1081 ctgaatcccg gtgtcggaga cgatgtcgtg cgtcggggag cggtcatcat ccaagccgcc
     1141 cccggcagcg aactcgacag ctggcttcgc aagggccgcg cctccgccgc catcgtgcgg
     1201 ccggaccgcg tggtgatgca ggccggccgg gacgcagaag cgatctgcgc cgccgtgcct
     1261 acgttctgcg caggcaagcc cgagaaaacc gaaggctgac gtctatgacc gaattgctgg
     1321 tgctcaacgc cggcaagggc gagatgcaag cggtttcgcc ggatgggacc cgggtccgca
     1381 ccttgatcgc tgggctcgac gaattgcctg acggcatcgt cgtcgaccag cggcggggcc
     1441 acgtgtactg gaccaacatg ggcgcgcccg atcccggttc cggcatgccc ggagacgcgg
     1501 cgtacacccg caacggctcg ctggagcggg ccgatctcga cgggggcaac cgtgtcacca
     1561 tcgtgccgcg ctcagcgttc accacgggca aacagctcac cgccgacttt gacgacggaa
     1621 cgctgtattg gtgtgaccgc gaagggatgc gcgtgctcag ctgcaagctc gacggcagcg
     1681 gtctgcgcac actcgtggtg tcgggggtgg gcgctgaggc ggcccgccat gcacgcaacc
     1741 accctgtcgg catcgccatc gatgtggatc gccggctgct gtactggaca caaaagggtg
     1801 ctccaaatgg cggtgagggc agaatctttc gtggcccgct ggatccggca gtcggtgtca
     1861 gcacgccgta cggcgacggt gtagaactgc tttggaagga ccttcccgag cccatcgatc
     1921 tgcacctcgt cgacgatggc gcgacgctgc tctggaccga tcgtggcgat cccgaaggca
     1981 atacgctcaa ccgcgcgatc gtgcagccga cggtcgaggc attcgaagtc ttgtcaaccg
     2041 cataccggga ggcgatcggt gtggccgcgg tgtccgaaac cgagttctat gtcagtgatc
     2101 tcggcggcag cgtccgtcac atcgatctgg ccagtggcac tgacatcgaa ctggtgaacc
     2161 tcggctcacc cctcaccggc atcgcgctcg ccgagctgtg agctacgaaa gtggttgatc
     2221 atcaacagat ctcgcggcgc tgggcggtga ctcggcccac ccagcgctgc gttaattccc
     2281 gggttgcttg tggccggtcc tacatggagc agggtgtgcc caaatgaccg agtggttcga
     2341 catctggcgc aatttaacgc cgggtcaggg cacgcttttc ggtggcgcgt tcgtcctttg
     2401 cgcgggcatc ctgacgttcg gcacagggtc actcgaccgc aaatcgcggg ctaagcgctt
     2461 gttctacgaa gagatgaaag cgctttacgc tcaggcactg agaatcggca gggatctgga
     2521 gctcctaaag gcggttccgc cggacggccg cgaaaagatc gtcaccgaaa agatcgatgc
     2581 gatgcagaag gtgatcagcg agctcgctct caccggtaac tatcaaacag cggacttggc
     2641 gatcgcgtac acatatcagc agtctgtcca gttggccgtg tggtcaagag aagttgaaca
     2701 cgacattggc ctgccggacc aggtgcggcg gtggctggac aatatgaccg acgaagaact
     2761 cgccgcattg aaggagtacg gaaacgtccg gatcgatcgt cgagacgtcg tcgaggcgct
     2821 gcgcgccgag ctggcgctat atgtccctcg gtggtctcgg tatcgacggg ttatgcgccg
     2881 accgcttcac gtcgacagat acgtcttgaa ctgacgtggg gccaagcgca cgccgctagc
     2941 cgtcgccgcc tgcctcgctt cctggcgtgc gcaggccccg ccgtgcgcct tgctaaccgg
     3001 aatcagcttg accagcaggc ttttccaacg ccaagggcgg cgcggcgagc ggcaactcta
     3061 cgcgcaggct ggtacctgcc ccgagggggc tgtcgagaaa gatccggccg ccgatcgcct
     3121 ccactcggtc cttgacgccc accaggcccg tacccgcggc gaagtgtgca tcgccgacac
     3181 catcgtcgct gacggcgatg tgaaggacgc cgtcggcggt gtcggttatg gcggcgacac
     3241 tgacggtgga tgcacgcgag tgcttggcca cattggtcag tgcctccgcg acgatgtagt
     3301 aggcgctggc ctcgacatgc tcgggcagcg gccgcgggat gcgcatgtcg aggtggacgg
     3361 ggatcgggca gcggcgggta agcgcgggca gcgccgcggt gagcccgccc ttggtaagga
     3421 tcgccgggtg aatgccccgc gcgatctcgc gcagctcgtc cagcaagtcg gacgcagccg
     3481 tgacagcgcg atcaacctgc gcgctgagct cagtaagctc ggaaggtacc accgcctgcg
     3541 ccatgcgcag ctccagtacc agcgaaacga gccgttgctg ggtcccgtca tgcaggtctc
     3601 gctcgaagcg ttggcgcgct tggtcgccgg cggcgacgat tcgggcgcgc gacgccgtca
     3661 actccgcctg tgcgtcggcg ttcgcgatcg cgatcgcgac cagctcggtg aagtcgccca
     3721 gccgggcttc ggtttccggg ggcggcggct cgtcggtcgt cgatgccacg acgatgagtc
     3781 cccatggacg gctcttgacg gtgatcggcg ccccgacggc ggtgcggacg ccaagctgga
     3841 aagccgccga gcccgcgtca ccctcgtagc ggtcgacgcg gacagggcgc ccggtctcac
     3901 gcacacgcgc actcacggtg cccgcgctca tgctgccgcc aatttgggtc gttgtgccaa
     3961 gaggcagcgc ctcgccggtt gcactccacg cggcgacgag cgtcgcggcg tcgtcggcgt
     4021 cgtaccgcgc cacgtatgcg cggtccactg acagaaggcg gccgacttcc tctgcgacgc
     4081 cggcgaatac ggcgttcggt ggggccgcag ccgcgacttg cgttgccacc cgccgcaacg
     4141 ccgcctgttc gtctgcgact cgccgcagcg cctcgcgagc ctccgcgttt gcgaccgcgg
     4201 tcgccaccag atccgtgaat tgtgccgtgg cgagctcgct tccggccggc agccgttccg
     4261 gatcggtcga aacgatggcc aggacgcccc agagcccgcc ctccacagtg atcggcgcgc
     4321 cgacgccgca tcgggcgccg caatcggcga agaacgcggc gatcgagccg ggcgggccct
     4381 cgaatcgatc gatgcgggcg gggcgtcgcg tgtcatgtac ctgcacgacg acacccgtgc
     4441 ctgcgacgtt gaagcgcgac ccgatcggaa tttcgatgcc ggggatgctc cagccgccga
     4501 ctagggttgc ctctcggtcc ggcgcaaagc ggaggacggc cgcgacatcg ccgccgaaga
     4561 gctgccctac ctctgcggcc acggcggaga acgtctcggt cggcgaggcg ccctgcgcga
     4621 caagcgtcgc tactcggcgc aacgctgctt gctcctcctg gccacgccgc tgctcggtga
     4681 tgtcgcgggc gaccgcgcag gcgaaccctt cctcaggtac cgcgcgagcg gtccactgga
     4741 gccatcgcag agagccgtcg gagcagatat agcggttctc gaacccgatc agttctgcac
     4801 cgctcgtttg ggaggcgaac gcgtccgccg tgagctcacg gtcatcgggg tggacgaact
     4861 caacgaacgg acgcgacgtg agctcggcga acgtataacc aagcgtccgc tcgaaggccg
     4921 ggttcacgcg cgtgaagtag ccgtcgaaat tgccgatgca tagcaggtcg accgacagct
     4981 ggaaaaagtc gctgagctcc cgttctgcgc gctcgcgccg gccctccaat tcggcgaagg
     5041 ccgccatggt gtcattgagg ccggtcagca gctgagacca tgttccctcg aaggcagcga
     5101 tgtttgcgcg atgctccagc cggttgtctc tcacggcggc ggtcatttcg cgtatctcca
     5161 cggccaggcg ctccaccgtc acgtgtaggt tgcggaaagc ggttgcgacg tcgccgattt
     5221 cgtcccgacc gcggtaaccg acctggacgg tcaggtcgcc gctggacagg tgttgggcgg
     5281 cttcggagac ctcacgcaac gggctggtga tcgatcggcg cagtgccagg gccagccccg
     5341 tcacgatgag aaggaccgca agcgagatgc cgacgtcgcg caggctgcgg gtgcgcgccg
     5401 cggcaaggtc attggaggcg gtcgcggcca gattattcct agccccgtcc tgcaggtccc
     5461 gcagggacgc aatccgcgtg ccggatgctt cgacccatcc agcgagggac ggttcccgca
     5521 gtgcgtcgag cgttcggttg gcgagggcat cgcgcacgcc ggtgaccgtg atgcccgaag
     5581 gactgaacag cacagcctcc aggtcggcag caagcgggcc ggtggcgttg tcgcggaaaa
     5641 cgtcgaactg cgcggcctcg aggccgctcc accgcgtggc gctcgaggcc gcgagcgcat
     5701 ccgcgggctc tgaagccaac aatgccgcga cgtcgacctg ctctcgtgcg gcggcctcga
     5761 ttgcctcgac aatcgagacg taagcctgag aggcccgcgc cgacggtcgc gtgggtgcgg
     5821 ccgcgtccag ctgccgtacc gtccggagca ggtcg
//
LOCUS       DASRJA010000268         3462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3999732,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000268 DASRJA010000000
VERSION     DASRJA010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3999732"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFC01_12005"
     CDS             complement(<1..761)
                     /locus_tag="VFC01_12005"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN80396.1"
                     /translation="MPLADGDVIAGYTILRSLGAGGMGEVYLAQHPRLPRRDALKVLS
                     ASVCSESEYRQRFNREADIAATLWHPHIVEVHDRGDVDGQLWISMDYVEGTDAGRLLA
                     QRYPDGMPADDVVRIVTAVADALDYAHQRGLLHRDVKPANILMSNTDTENERIMLADF
                     GIARWVGESSALTGTNMTVGTVAYSAPEQLKGEEIDGRADQYALAATAFQLLTGTPPF
                     QHSNPAVVISQHLTADPPAIGTRRPELSSLGPVFD"
     gene            complement(783..2348)
                     /locus_tag="VFC01_12010"
     CDS             complement(783..2348)
                     /locus_tag="VFC01_12010"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HZN80397.1"
                     /translation="MTVEPSPTTTAEKLAELREKLELAKEPGGEKAVAKREKKGIPSA
                     RARINALVDPGSFLEIGALCKTPGDPNALFGDGVVTGHGTINGRPVGVFSHDQTVFQG
                     SVGEMFGRKVAKLMEWVAMVGCPIIGINDSAGARIQDAVTSLAWYAELGRRHELLRGL
                     VPEISIILGKCAGGAVYSPIQTDLVVAVRDQGYMFVTGPDVIKDVTGEDVTLDELGGA
                     DAQASFGNIHQVVEDEAAAFQYARDYLSFLPANTFDDPPIVNPGLEPEITPHDYELDA
                     IVPDSDNMAYDMHEILLRIFDDGDVFEVAQQQGPAMITAFARVDGRPVGVIANQPMHM
                     SGAIDNEASDKAARFIRFCDSYNTPLVFVVDTPGFMPGVEQEKGGIIKRGGRFLNALV
                     EADIPKVTITIRKSYGGAYAVMGSKQLAADLNFAWPTARIAVIGAEGAAQLLVKRFPD
                     PTAPEVQKIRADFIEGYNLNMATPWIAAERGYIDAVIQPHETRLLLRKSLQLLRDKQK
                     HGRVQRKHGLLPI"
     gene            complement(2345..>3462)
                     /locus_tag="VFC01_12015"
     CDS             complement(2345..>3462)
                     /locus_tag="VFC01_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HZN80398.1"
                     /translation="LTQEAVTEALGADVPPREAAERVTFATWAIVTGKSPGGIFNALP
                     TLDDETAEKMAQRLSERAEGTITAEDIKNSPTIEALASIVREHLEAGEVDGFVRTLRA
                     PQEGSDRTPVFVFHPAGGSTVVYEPLLKRLPADTPMYGIERVEGSIEQRAAEYVPKLL
                     EMGGNGPFILAGWSLGGAMAYACAIGLKRAGADVRFVGLIDAVRPGEEVPQTKEETRA
                     RWDRYARFAERTFNVQVPPIPYEQLEELDDEGQVQLVLDAVSQSGVQIPGGIIEHQRT
                     SYLDNRLLETAKIEQYDGHVTLYMADRYHDDAIFFEPRYAIRQPDGGWGEFVSELEVV
                     PIGGEHIQAIDEPYIAKVGAHMSEAINRIEAEEHQAK"
BASE COUNT          546 a         1178 c         1109 g          629 t
ORIGIN      
        1 ttgtcgaata ccggtccaag gctggacaac tcgggacggc gggtcccgat cgcaggcgga
       61 tccgccgtca ggtgctggct gatcaccaca gcggggttgg agtgttggaa gggcggcgtg
      121 ccggtcagca gctggaaggc cgtcgccgcg agcgcatact ggtcggcacg tccgtcgatc
      181 tcttcaccct tgagctgctc gggtgccgaa tacgcgacgg tgcccacggt catattggtg
      241 ccggtgagcg cgctcgactc acccacccaa cgggcgatcc cgaagtcggc caacatgatt
      301 cgctcgttct cggtgtcggt attggacatc aagatgttgg ccggcttcac atcgcggtgc
      361 aacagcccgc gttggtgcgc gtaatcgagc gcgtcggcga ccgcggtcac gatccgcacg
      421 acgtcgtcgg ctggcatgcc atccggatag cgctgcgcga gaaggcggcc cgcatcggtg
      481 ccctcgacgt agtccatcga gatccacaac tggccgtcga catctccgcg gtcgtgcacc
      541 tccacgatgt gcgggtgcca cagcgtggcc gcgatgtcgg cctcccggtt gaaccgttgc
      601 cggtactcgc tttccgagca caccgacgcc gacagcacct tgagcgcgtc gcgtcgcggc
      661 agccgcggat gctgggccag atagacctcg cccatcccac ctgcaccgag cgatcgcagg
      721 atggtgtacc ccgcgatcac gtcgccgtcg gctagcggca tcggcgcacc cttcgccgta
      781 gcctagatcg gaagcaaccc atgcttgcgc tgcacgcggc cgtgcttctg cttgtcccgc
      841 aacagttgta gtgatttgcg cagcagcaga cgggtttcgt ggggttggat caccgcgtcg
      901 atgtatccac gctcggcggc gatccacggc gtcgccatgt tgaggttgta gccctcgatg
      961 aagtcagccc ggatcttctg cacctccggc gcagtcggat cggggaaccg cttcaccaac
     1021 agctgagcgg caccctcggc accgatcacc gcgatgcgcg ccgtcggcca cgcgaagttg
     1081 aggtcggcgg ccagctgctt ggaccccatc accgcgtacg caccgccgta ggacttgcgg
     1141 atcgtgatgg tgaccttcgg gatgtcggcc tccacgagcg cgttgaggaa ccggccaccg
     1201 cgcttgatga tcccgccctt ctcctgctcg acgcccggca tgaatcccgg ggtgtccacg
     1261 acgaaaacca gtggggtgtt gtatgagtcg cagaacctga tgaaccgggc ggccttgtcg
     1321 gaggcctcgt tgtcgatcgc gccagacatg tgcatgggct ggttggcgat cacaccgacc
     1381 ggccgcccgt cgactcgcgc gaatgcggtg atcatcgcgg gtccctgctg ttgagccacc
     1441 tcgaacacgt cgccgtcgtc gaagatccgc agcaggatct cgtgcatgtc gtaggccatg
     1501 ttgtcactgt ccggcacgat cgcatcgagt tcgtagtcgt gcggtgtgat ctccggctcc
     1561 aggccggggt tcacgatcgg cggatcgtca aacgtgttgg ccggcaagaa actcaagtag
     1621 tcgcgggcat actgaaatgc cgcagcctcg tcctcgacca cctggtggat gttgccgaag
     1681 ctggcctgcg cgtcggcgcc gcctagttcg tcgagagtga cgtcctcacc ggtgacgtcc
     1741 ttgatgacgt cggggccggt gacaaacatg tagccctgat cccgcaccgc cacaacgaga
     1801 tccgtctgga tcggcgaata taccgctccc ccagcgcatt tgccgagaat gatggagatc
     1861 tcaggaacga ggccacgcag cagctcatgg cggcggccca gctcggcgta ccacgcaagc
     1921 gaggtcactg cgtcctggat gcgggccccg gccgagtcgt tgatgccgat gatcgggcag
     1981 ccgaccatcg ccacccactc catcagcttg gccaccttgc ggccgaacat ctcgccgacc
     2041 gagccctgga acacggtctg gtcgtggctg aagacgccga ccggcctgcc gttgatggtg
     2101 ccgtgcccgg tgaccacgcc gtccccgaag agcgcgttgg gatcacccgg tgtcttgcac
     2161 agcgcaccga tctccaggaa actgcctggg tcgacgagcg cgttgatgcg cgcacgggca
     2221 ctcggaatgc ccttcttctc acgcttggcg acggccttct ccccgccggg ctccttggcc
     2281 agctcgagct tctcgcgcag ttcggccagc ttctcggcag tcgtggtggg agacggttct
     2341 acggtcactt cgcctggtgc tcctcggcct cgatccggtt gatcgcctcg ctcatatgcg
     2401 cgccgacctt cgcaatgtac ggctcgtcga tcgcctggat gtgctcgccc ccgatcggga
     2461 cgacctccag ctccgagacg aactcgcccc agccgccgtc gggctgacgg atggcgtagc
     2521 gaggctcgaa gaagattgcg tcgtcgtggt agcggtcggc catgtaaagc gtgacgtgcc
     2581 cgtcgtactg ctcgatcttc gcggtctcga gcaggcggtt gtccaggtag gacgtccgct
     2641 gatgctcgat gatcccgcct gggatctgca caccgctctg gctgaccgcg tcaagcacga
     2701 gctgcacctg accctcgtcg tcgagctcct cgagctgctc gtacgggatt ggcgggacct
     2761 ggacgttgaa ggtccgctcc gcgaaccggg cgtaacggtc ccagcgggcg cgggtctcct
     2821 ccttggtctg cgggacctcc tcgccgggac gcaccgcgtc gatcagcccg acgaagcgga
     2881 cgtcggcgcc cgcacgcttc agcccgatcg cgcacgcgta ggccatggca ccacccaacg
     2941 accacccggc caggatgaac gggccgttgc cgcccatctc cagcagcttc ggtacgtact
     3001 cggcggcgcg ctgctcgatg gagccctcca cccgctcgat gccgtacatc ggggtgtcgg
     3061 ccggcagccg cttaagcagc ggctcataca ccaccgtcga gccaccggcc ggatggaata
     3121 cgaacaccgg ggtcctgtcg gaaccctcct gcggcgcgcg cagggtgcgg acgaagccgt
     3181 cgacctcacc cgcttccagg tgctcgcgca cgatgctcgc cagcgcctcg atagttggcg
     3241 agttcttgat gtcctcggcc gtgatggtgc cctcggcccg ctccgaaagc cgctgtgcca
     3301 tcttctcggc ggtctcgtcg tcgagcgtgg gcagcgcatt gaagatgcca cccggcgact
     3361 tgccggtgac gatggcccac gtcgcgaacg tgacacgctc ggcggcttcg cgcggcggca
     3421 cgtcggcgcc cagcgcttcg gtcaccgcct cctgcgtcag ca
//
LOCUS       DASRJA010000269         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_801317,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000269 DASRJA010000000
VERSION     DASRJA010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801317"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFC01_12020"
     CDS             complement(<1..331)
                     /locus_tag="VFC01_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HZN80399.1"
                     /translation="MLAATAVSQSATDPLSGLVLGDVADPQPKPGCAVIRVVSSSLNM
                     HDLWTLRGVGHPAENLPIVLGCDAAGYDEDGNAVIVYPVIGDHDAGGGDETLDPDRAL
                     LSERHDGA"
     gene            619..1791
                     /locus_tag="VFC01_12025"
     CDS             619..1791
                     /locus_tag="VFC01_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZN80400.1"
                     /translation="MFAASWLFLAHTDQFHKPGDFFTTYMGEDPVIVTMDKNRRIRAF
                     LNSCRHRGARVCRADYGATRSFTCTYHGWAYDLEGKLISIPNKEGYPDSFHADDWGLV
                     EVANVDSYHGLIFGTWDPDPVSLRDSLGDMAWYLDAMLDHDAEGTVVVGGVHKWMLEG
                     NWKLAAEQFASDWYHVNMSHASALTVLSPSGKPKDEVVHRTGRQFVDPMGHGHGFPVH
                     PKNRFDSQTVHEWIDYAALRERLGDARVEGPVTTGHGTVFPNFSYLPVNGSIRIWHPK
                     GPDRMEVWAWTIVDKSMPEHVREAQRLYNLRTFGPSGIFEQDDGENWSEVQAISHGFI
                     TNSVPLNYQMGIGADQRDTRYPGTTSELYSDAAARSFYARWRELMDTSAWHERKPA"
     gene            1788..2123
                     /locus_tag="VFC01_12030"
     CDS             1788..2123
                     /locus_tag="VFC01_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016894996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HZN80401.1"
                     /translation="MSNGIRVAALDDIPEGEGISIDRTVTGTDDDIALLRDDDGSVWA
                     LNNTCTHAEASLAEGWVEDGYVECPLHSSKFCLKTGTVQGLPATRDAVAHRIEVREGD
                     VYLFPHERP"
     gene            2126..>2313
                     /locus_tag="VFC01_12035"
     CDS             2126..>2313
                     /locus_tag="VFC01_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN80402.1"
                     /translation="MARVVVAGGGIAGVSTVSALRAGGFAGDLTLVDAGEFPYDRPPL
                     SKDYLAGRTELKSIALQTP"
BASE COUNT          432 a          724 c          760 g          397 t
ORIGIN      
        1 acgcaccgtc gtggcgttcc gacaacaatg ctctgtccgg gtccagtgtt tcgtcaccgc
       61 cgccggcgtc gtggtcgccg atcaccgggt agacgatgac cgcgttgccg tcctcgtcgt
      121 aaccggcggc gtcgcaaccg agcacgatcg gcaggttctc tgccggatgg ccgacgcccc
      181 gcagggtcca caggtcgtgc atgttcagcg aggatgaaac aacccggata actgcgcacc
      241 cgggcttggg ttgcgggtcg gcgacgtcgc ccagcacgag accggacaac gggtcggtgg
      301 cgctctggga gacggctgtt gcggcgagca cggcaccaca atcgcgcccg agatccactg
      361 aaaaccagcc gctgtgccgc tgtgcgaaac gtgggatgga acttggcctg cccacggagt
      421 ttcgtgtgtg gcatgacatc aacgatcgaa ccgggcaccg gcgcgcggac ggtcagtgca
      481 ccaccctgcg gtgccctgca gcggcccgtg gaattggacc gcaacatcga cgtggccggg
      541 ctggtcgacg ccgacggcgg actgctggat cgcgcggtgt tcaccgacga cgagatctac
      601 cggcaggaac tgcgccgaat cttcgccgcc agttggcttt tcctcgcgca caccgatcaa
      661 ttccacaagc ccggcgactt cttcaccacg tacatgggcg aggacccggt catcgtcaca
      721 atggacaaga accggcggat cagagccttc ttgaactcgt gccgtcaccg cggggcgcgg
      781 gtctgccgag cggactacgg cgccacccgc agcttcacct gcacctatca cggctgggcg
      841 tatgacctgg aaggcaagtt gatcagcatc ccgaacaagg agggctatcc ggacagtttc
      901 cacgccgacg actgggggct cgtcgaggtc gcgaacgtgg attcgtatca cgggctgatc
      961 tttgggacct gggatcctga tccggtctca ctacgagatt ccttgggcga catggcctgg
     1021 tacctggatg cgatgctcga ccacgatgcc gagggcacgg tcgtggtcgg cggtgtgcac
     1081 aagtggatgt tggaaggcaa ctggaagctg gccgccgagc agttcgccag cgattggtat
     1141 cacgtgaaca tgagccacgc ctcggcgctg acggtgcttt cgccgagcgg aaagcccaaa
     1201 gacgaggtgg tgcaccggac tgggcgccag ttcgtcgacc ccatgggcca tggccacgga
     1261 tttccggtgc accccaagaa ccggttcgac tcacaaacgg tgcacgagtg gatcgactac
     1321 gccgccctgc gcgaacgcct cggtgacgcg cgggttgagg gcccggtgac aaccggtcac
     1381 ggcaccgttt tccccaactt ctcttacctt ccggtcaacg gctcgatccg gatctggcac
     1441 ccgaaaggtc ccgaccggat ggaggtctgg gcgtggacga tcgtggacaa gtcgatgcct
     1501 gagcatgtgc gggaagcgca gcggctgtac aacctgcgga ccttcgggcc gagcggcatc
     1561 ttcgaacaag acgacggcga gaactggagt gaggtgcaag ctatttcgca cggtttcatc
     1621 accaactcgg tgccgctgaa ctatcagatg ggtatcggcg ccgaccaacg cgacacccgc
     1681 taccccggca ccaccagcga gttgtacagc gacgcggccg cgcgttcgtt ttacgcgcgg
     1741 tggcgcgaac tgatggacac atctgcctgg cacgagcgga aaccagcatg agcaacggca
     1801 tccgagtcgc agccctcgac gacatccccg aaggggaggg catctcgatc gaccgaacgg
     1861 tcaccggtac ggacgacgac attgctctgc tgcgtgacga cgacggcagc gtgtgggcct
     1921 tgaacaacac ttgcacgcac gccgaggcct ccctcgccga gggctgggtc gaggacggct
     1981 atgtggagtg cccgctgcat tcaagcaagt tctgtctgaa aacgggtacg gtgcaggggc
     2041 ttccggccac ccgcgacgcg gttgctcatc gcattgaggt acgcgaaggc gacgtctacc
     2101 tgttccccca tgagaggccg tgatggtggc cagggttgtg gtcgcagggg gcggcatcgc
     2161 cggcgtcagc accgtctcgg cgctgcgggc tggcggattc gccggtgatc tgacattggt
     2221 ggatgccggc gaatttccgt atgaccggcc accgctgtcg aaggactatc tcgccggccg
     2281 taccgagctg aaaagcattg ctctgcagac gcc
//
LOCUS       DASRJA010000270        11090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_175515,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000270 DASRJA010000000
VERSION     DASRJA010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11090
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175515"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VFC01_12040"
     CDS             complement(<1..1085)
                     /locus_tag="VFC01_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HZN80403.1"
                     /translation="MSTVDGKQALRPAIVIGALGVVFGDIGTSPIYTIQTVFNPGDPH
                     PVPISTDNVYGVVSTIFWSVMIIVTLTYITLVMRADNNGEGGIMALITLLRRSTGHRS
                     RRTAMALAALGLFGASLFFGDSMITPAISVLSAVEGLKVIEPDFEDFIVPITAVIIVG
                     LFAVQRHGTAAVGRFFGPVMILWFTAIGACGVLGVVDNPEILKALSPTYAATFLLGHF
                     HIAFFSLAAIVLSVTGAEALYADMGHFGRKAITYGWLGLVLPACTLSYFGQGALVLAD
                     EAKVIAPFFLLTPEWARIPMVLLATAATVIASQAVITGAFSVASQAAQLGYLPRLRIE
                     HTSASTIGQIYVPWINGVLMVMVLILV"
     gene            1188..2009
                     /locus_tag="VFC01_12045"
     CDS             1188..2009
                     /locus_tag="VFC01_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80404.1"
                     /translation="MADELDELYEAKPEEFTALRTKLAAAAKQSGDVDTAKQISAARK
                     PTTAAWVVNRLALRDRDIRTRLAGLGERLKDAHAAMDGDRIRELTREQRRLIDDVARA
                     AFEEAELANPAASLRDDVTGTLQAAVADPDVASRLGRLTKAEEWSGFGEFGDTAMVFT
                     ATRKSAARKKSAEAPQRDEQPDEQPDKRDAARRERDKARAAVAEAERAKSEADAALTE
                     LQADVATARLRRDDARRRLKDAEDALAAAEDAYDKGKQASREAAAVVKEAKAHLR"
     gene            2019..2738
                     /locus_tag="VFC01_12050"
     CDS             2019..2738
                     /locus_tag="VFC01_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN80405.1"
                     /translation="MDVHVADGVTTVRIDHPPVNAFDMGLVEDAVATVRGIDGPIVIT
                     GAGSCFSAGVDLRAIIDGPPEYTDRFLGAVSDAFLAVFDHPAPVVAAINGHAIAGGCV
                     LAMAADVRLMSSGLIGLSEVAVGVPFPVAALEICRYAMGPPVTRAVLQADNIDVRSAA
                     ERGWIDEVVAPDDLLTRAIGIARQLGQHPPTAYAAMKEQLHRHARDAIDVGAELDAKV
                     RADWKSDETRARISAFLDALK"
     gene            complement(2731..3396)
                     /locus_tag="VFC01_12055"
     CDS             complement(2731..3396)
                     /locus_tag="VFC01_12055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN80406.1"
                     /translation="MTTTLQDARVATALDRMYSESTEQFTQLRERSGDFERLASASAQ
                     ERADAFSDFYLPVTPDAGRLLYALVRATQPDTVVEFGMSLGISAIHLASAVRDNGTGR
                     VVTTELSAAKVAKAKETFAETGLDDLITVLEGDALTTLADVGGPVGFVLLDGWKELYL
                     PVTKLLEPKLSPGALIVADNTSMDDTKPYLEHVRDPGNGYVSVNFPVREGDSMEISCR
                     AIT"
     gene            3484..4446
                     /locus_tag="VFC01_12060"
     CDS             3484..4446
                     /locus_tag="VFC01_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HZN80407.1"
                     /translation="MAGIDKSTARVIAVITLLFVTAWALRGYLPGIEPAADRQRPGSS
                     PAALVVDIVLLSISVAIIGLAIITRLRNREARRPGPGQLPGSRGTMGRPTWRLSLIAI
                     ALAIAWLLVVMLLMQLGIGEPGEQAPSGTPTVADPGSAPTTTTNPAPPQPDSSDNPGP
                     NVVGYLIPPMLILMTLIVVGTAIVSRRQRRLVTAWPPAHGDVPETSARAGAAESLARA
                     AEVGLAEIGDLSREPREAIIACYAAMERELTHVPGAAPLDCDTPSEVLARAVDHHALH
                     APSATQLVELFEEARFSPHVMNEGHRDTAFDALRLVLADLRSMA"
     gene            4443..4928
                     /locus_tag="VFC01_12065"
     CDS             4443..4928
                     /locus_tag="VFC01_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80408.1"
                     /translation="MKKLVAAGIFIVALVEVVAFAVLDRELVLVVVGVMVGLVLLALR
                     YYLVHEPGYDSGDALGNDPAESLRRWLSRTETLISRSDSTRRDWDRHLRPMLARQFEL
                     ATGQRQTRNRTAFHVTGTMLFGAELWAWVDPQNVSRTGDSEPGPGRDALDEILRRLEQ
                     V"
     gene            4925..5884
                     /locus_tag="VFC01_12070"
     CDS             4925..5884
                     /locus_tag="VFC01_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN80409.1"
                     /translation="MTMPAATTTAHCESVLDEIGRAVVGKRGALTLILTTVLAGGHVL
                     IEDLPGLGKTLIARSFAAALGLDFTRVQFTPDLLPADLLGSTVYDMQSGHFEFRRGPI
                     FTNLLLADEINRTPPKTQAALLESMAEGQVSIDGVTHKLPAPFIVLATDNPIEYEGTY
                     PLPEAQLDRFSIRLELRYLSEQEEASMLRRRLERGSAQPIVHQVVDGHDLLAMRESVE
                     QVTVHDDVLGYVVSLATATRQHPQVAVGASPRAELDLMQLARARALLLGRDYVIPEDV
                     KYLAVPTMAHRISLRPEMWVRRVHGSDVVEELLRRLPVPRTKG"
     gene            5889..7157
                     /locus_tag="VFC01_12075"
     CDS             5889..7157
                     /locus_tag="VFC01_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZN80410.1"
                     /translation="MIETHSTEVELRWRPAPLTRSVVTCAAAALAVAVIGRYWQLVAF
                     AAPLIGVLVSIGWQREVSKVHVHAEPGGQRCFESEETRVRVSAIAETPDVDVHLTLEA
                     VDGMRLETVELQSPQRQTVAVAADTWGRYPIVARVNVLARGGLVLGSGAADAANIYVF
                     PLAPPQSTAIPRTELLDRLGTHLTRHIGPGVEYADVRRYVPGDQLRTVNWPVSARRGS
                     LHVTERLTDRAADVVVLIDGYPQPPGPATEATDRVVRGAVQVVQSALRSGDRAGIVAL
                     GDRHPRWLGADIGRRQFYRILDAVLTAGEDFETTPGTLAPQAALPPGAIVIAFSTLLD
                     TEFALALIDLRKRGHTVVAVDVLEGAPFDEECDPLIARMWSLQRSFMYRDMRTIGVDV
                     VSWHGDATLDQAMQLVPDHRRSARRRAALR"
     gene            complement(7246..8490)
                     /locus_tag="VFC01_12080"
     CDS             complement(7246..8490)
                     /locus_tag="VFC01_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZN80411.1"
                     /translation="MKSARNTLPTEPANGVTGGIDWARDDHAVSIVDDRGREIVRSTV
                     EHNAAGLRELLVVLERAGVAEVAIERPDGPVVDTLLDAGITVVVISPNQVKNLRGRYG
                     SAGNKDDRFDAFVLADTLRTDRARLAPLLPDTPATITLRQTSRARKDLVAHRVAVANQ
                     LRAHLQIVFPGAVGLFAALDSPISLAFLTRFDCQDRADWLSINRLAAWLASVGYSGRT
                     DPAVLHRRLTSAPRGTAGTDHTATTAAFVAVLSALTAQIKALDDDIAQQLARHADSQI
                     FTSLPRSGTVRAARLLSEIGDCRARFPTPESLACLAGVAPSTRQSGKVKHVGFRWAAD
                     KQLRDAVTDFAGDSRRANPWAADIYNRAIARGHDHPHAVRILARAWLYVIWHCWQDEV
                     AYDPAKHRALQTLLNQDQPRAA"
     gene            8569..8748
                     /locus_tag="VFC01_12085"
                     /pseudo
     CDS             8569..8748
                     /locus_tag="VFC01_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899976.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8799..9173
                     /locus_tag="VFC01_12090"
     CDS             8799..9173
                     /locus_tag="VFC01_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80412.1"
                     /translation="MVASAAVQADRAGLIAAVVAAAAVLAGSVFRPAATVAVLVTVVV
                     IVLTDAPPVLTATAGLCAAAYLVLRHTAAVTAPTVLGAVGFTAAGLAAASVSIEMPWV
                     PLLAPLAVLVVYVLVTRPFWAT"
     gene            complement(9148..9837)
                     /locus_tag="VFC01_12095"
     CDS             complement(9148..9837)
                     /locus_tag="VFC01_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HZN80413.1"
                     /translation="MDFPPIVSPQEWDTARQELLVKEKELTRARDALAAQRRRMPRML
                     VEKDYLFDGPGGPASLLDLFDGRRQLIVYRFFYQPDVDGWPDRGCPGCSMLADQVGHL
                     AHLNARDTTLVFVSRAPRDAIARLKKRMDWEIPWYSLTDSFDADFGVDEWHGTNAFLR
                     DDGQIFRTYFIDNRGDEAMGSTWAYLDITALGRQEEWEDSPDGYPQTPPYAWWRRHDE
                     YPAAMSPRTGE"
     gene            9979..>11090
                     /locus_tag="VFC01_12100"
     CDS             9979..>11090
                     /locus_tag="VFC01_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN80414.1"
                     /translation="MPNDVRKAVTGASIGNAVEWFDFAIYGFLATFIAANFFPSGNET
                     AALLNTFAIFAAAFFMRPLGGFVFGPLGDRIGRQRVLALVILLMAAATVAIGLLPTYA
                     TIGVAAPLLLLFLRCLQGFSAGGEYGGGAVYLAEFASEKHRGLTVTFMVWSGVLGFLL
                     GSVTVTLLQALLPAGAMESYGWRIPFLIAGPLGLVGLYIRLRLDDTPQFAELSKAEQV
                     AKSPLREAITTASRPILQVIGLMIIFNIGYYVVFTFLPSYFIKTLHFSKTNAFVSITL
                     ASLVALILILPLAALSDRVGRRAMLITGALAFAILGYPLFLLLNSGSLAAAIAAHCGL
                     AAIEAIYVSTAVAAGVELFATRVRYSGFSIGYNVCVA"
BASE COUNT         1783 a         3616 c         3818 g         1873 t
ORIGIN      
        1 aacacgagga tcagcaccat caccatcagc acgccgttga tccacggcac gtagatctgg
       61 ccgatcgtcg acgccgacgt gtgttcgatg cgcagccgcg gcaggtagcc gagctgggcc
      121 gcctgcgacg ccaccgagaa cgcgccggtg atcaccgcct gcgacgcgat gaccgtcgcc
      181 gcagtagcaa gcagcaccat cggaattcgc gcccactccg gggtgagcag gaaaaacggc
      241 gcgatcacct tggcctcgtc cgcgagcaca agcgccccct ggccgaagta gctcagcgtg
      301 caggcaggca gcacgagccc tagccagccg tacgtgatgg ccttgcggcc gaagtggccc
      361 atgtcggcat acagcgcctc ggcgccggtg acggatagca cgatcgccgc cagtgagaag
      421 aacgcaatgt gaaagtgccc caacaggaac gtcgccgcgt aggtagggga gagcgccttg
      481 aggatttcgg gattgtcgac gacgccgagc actccgcacg cgccgatcgc ggtgaaccag
      541 aggatcatca ctggcccgaa gaatcgcccg accgccgcgg tgccgtgccg ctgcacggcg
      601 aatagcccca cgatgatcac cgcggtgatc ggcacgatga aatcctcgaa gtccggctcg
      661 atgaccttga ggccctcgac cgcggacagc acggagatcg ccggggtgat catgctgtcg
      721 ccgaagaaca gtgaagcgcc aaatagtccg agcgcggcca gcgccatcgc ggtgcggcgg
      781 ctacggtggc cggtcgagcg gcgcagcagc gtgatcaagg ccatgatccc gccttcgccg
      841 ttgttgtcgg cgcgcatcac cagcgtgatg taggtcagcg tgacgatgat catcaccgac
      901 cagaagatcg tcgacacgac gccgtacacg ttgtccgtcg agatcggcac ggggtgcgga
      961 tcgccgggat tgaagacggt ctggatggtg tagatcgggc tggtaccgat gtcaccgaac
     1021 accacgccga gtgcgccgat cacgatggct gggcgtagtg cctgcttacc gtccacggtt
     1081 gacatcgttg cattgcagcc cgacgaacgt tccttaccgc tcggcaattc gccgagatat
     1141 cgagcggtac ggaacgttcg tccccatcac gcgcgatcat gggagccatg gccgatgaac
     1201 tcgacgagct gtacgaggcc aagccggagg agttcaccgc cctgcgcacc aagctcgccg
     1261 cagcggctaa acagagcggg gacgtcgaca ccgccaaaca gatctcagcc gcgcgcaagc
     1321 ccaccacggc cgcgtgggtc gtcaaccggc ttgcgcttcg cgaccgcgac atccgcaccc
     1381 gcctcgctgg gctcggcgag cggctgaaag acgcccacgc cgccatggac ggcgaccgca
     1441 tccgtgagct gacccgcgag cagcgccggc tgatcgatga cgtggcccgg gcggcatttg
     1501 aagaagccga gctggccaat ccggccgcct cgctgcgcga cgatgtcacc ggcaccctgc
     1561 aggccgcggt cgccgaccca gacgtcgcgt cgcggcttgg ccggttgacc aaggccgagg
     1621 agtggtctgg cttcggcgag ttcggtgata ccgcaatggt tttcaccgcg acccgcaaaa
     1681 gcgccgctcg caagaagtct gccgaggccc cccagcgcga cgagcagccc gacgagcagc
     1741 ccgacaagcg cgacgccgcg cgacgcgaac gtgacaaggc gcgggccgcg gtggccgaag
     1801 ccgagcgggc caaatccgag gccgacgcgg cgctgaccga gctgcaggcc gacgtcgcca
     1861 ccgcccggct gcgtcgtgac gacgcgcgca gacggctgaa ggacgccgag gacgccttgg
     1921 ccgccgcgga agacgcctac gacaaaggca agcaggccag ccgcgaagcg gcggcggtgg
     1981 tgaaggaagc caaggcgcat ctgaggtagg aatctgtcat ggatgtgcac gtggccgacg
     2041 gcgtgacgac cgtccggatc gaccacccac cggttaacgc tttcgatatg gggctggtcg
     2101 aggacgccgt cgccaccgtc cgcggcatcg acgggccgat cgtcatcact ggcgccggtt
     2161 cctgtttctc ggcgggcgtc gacctgcgcg cgatcatcga cgggccgccc gaatacaccg
     2221 accgcttcct gggagcggtc tcagatgcgt tcctcgcggt gttcgaccac ccggcgccgg
     2281 tggtcgccgc aatcaacgga cacgcgatcg cgggcggctg cgtcctggct atggccgccg
     2341 acgtccggct gatgtcgtca ggcctgatcg gcctcagcga ggtcgcggtg ggcgtgccgt
     2401 tcccggtggc cgccctggag atctgccgat acgcgatggg cccgccggtg acacgtgcgg
     2461 ttttgcaggc cgacaacatt gacgtgcgct ccgccgccga acgcggctgg atcgacgaag
     2521 tggtcgcccc ggatgacctg ctcacccgtg ccatcgggat cgcgcgtcag ctgggtcagc
     2581 atccaccaac cgcctatgcg gcgatgaagg agcagctgca ccggcacgcc cgcgacgcga
     2641 tcgacgtggg cgccgaactg gatgcgaagg tgcgggcgga ctggaagtcg gacgaaacgc
     2701 gcgcccgcat ctccgcgttc ctcgacgcgc tcaagtgatt gcgcggcagc tgatttccat
     2761 gctgtcaccc tcgcggaccg gaaagttcac gctcacatag ccgttgccgg gatcacgcac
     2821 atgctccagg tacggcttgg tgtcgtccat cgacgtgttg tccgcgacga tcagcgcgcc
     2881 cggcgacaac ttcggctcca gcagcttggt caccgggagg tagagctcct tccagccgtc
     2941 gagcagcacg aacccgaccg gcccgccgac gtcggccagc gtggtcagcg cgtcaccttc
     3001 cagcacggtg atcagatcgt cgagccccgt ctcggcgaac gtctcctttg ccttggccac
     3061 ctttgccgcg ctgagctcgg tggtgacgac gcgcccggtg ccgttgtcgc gaacggccga
     3121 cgcgaggtgg attgccgaaa tccccaacga catgccgaac tcgacgacgg tgtccggctg
     3181 cgtcgcccgc acaagtgcat acaacagccg gccggcgtcc ggcgtcaccg gcagatagaa
     3241 atcgctgaac gcgtccgcgc gctcctgggc gctggccgag gccaggcgct cgaagtcgcc
     3301 ggatcgttcg cgtaactggg tgaactgttc agtggattcg ctgtacatcc ggtcgagggc
     3361 ggtcgcgact ctggcgtcct gaagtgtggt ggtcactgcg ccaaagatag ccgcccaaaa
     3421 taagcgtcgg cagggtccac gtacgtgcca ggatggggat tagctcagcc gattggtgtg
     3481 ccgatggccg gtatcgacaa gtcgacagcg cgagtgatcg cggtgatcac gctgttgttc
     3541 gtcaccgcgt gggcgctgcg cggatacctg cccggcatcg aacccgcggc cgatcggcag
     3601 cggccgggca gcagcccggc tgcgctggtc gtcgacatcg tcttgctcag catctcggtc
     3661 gcgatcattg gcctcgcgat catcacgcgg ttgcgcaatc gtgaggcgcg gcgcccaggg
     3721 cccggccagt tgcccggaag tcggggcacg atgggccggc cgacgtggcg gctatcgctg
     3781 atcgcgatcg cgctggccat cgcgtggctg ctggtggtga tgctgctgat gcagttgggc
     3841 attggcgagc ccggggagca ggcaccgtcg ggaacgccga ccgtcgcaga cccaggcagt
     3901 gcgccgacaa ccaccacgaa cccggctccg ccgcagccgg attcgtcgga caatccgggg
     3961 cccaacgtcg tcggctacct catcccgccg atgttgatcc tgatgacgct gattgtcgtc
     4021 ggcaccgcga tagtgtctcg cagacaacgg cggctagtga cagcatggcc cccggctcac
     4081 ggtgatgtgc ccgagacgtc cgctcgggcc ggcgccgccg aatcactcgc gcgggcagcc
     4141 gaagtcggcc tcgcggagat cggcgatctg tcccgtgaac cacgcgaggc gatcatcgcg
     4201 tgctatgcgg cgatggagcg tgaactgaca catgtgccgg gcgcggcgcc cctggattgt
     4261 gacaccccgt cggaggtgct ggcccgcgcg gtcgaccatc atgcgctgca tgctccaagc
     4321 gcgacacagt tggtggaatt gttcgaggag gcacgctttt ccccacatgt gatgaacgag
     4381 ggacatcgcg ataccgcctt cgatgcgctg cggctggtgc tcgctgacct gcggagcatg
     4441 gcatgaaaaa gcttgtggcc gcaggcatct tcatcgtggc gttggtcgaa gtggtggcgt
     4501 tcgcggtgct cgaccgcgag ctcgtgctgg tggtcgtggg cgtgatggtt ggtttggtgt
     4561 tgctggcctt gcggtactac ctggtgcacg aacctgggta cgactccggc gacgcattgg
     4621 gcaatgatcc cgcggaatcg ttgcgccgct ggctatctcg gaccgagaca ttgatcagcc
     4681 ggtcggattc cacgcgcaga gactgggatc ggcacctgcg ccccatgctg gcccggcagt
     4741 tcgagctggc caccggacaa cggcagacca ggaatcgcac ggcgtttcac gtcaccggca
     4801 ccatgctgtt cggtgcggag ctgtgggcat gggttgaccc gcagaacgtg tcgcgtaccg
     4861 gcgacagcga acccggccct gggcgggacg ccctcgacga aatcctgaga cggttggagc
     4921 aagtatgacg atgccggcag cgacaacgac cgcccactgc gaatcggtgc tcgacgagat
     4981 tggccgggcg gtggtcggca agcgtggtgc gctcacactg attctcacca cggtgctcgc
     5041 gggcggccat gtgctgatcg aagacctgcc agggctcggc aagacactga tcgcccggtc
     5101 cttcgcggcg gcgcttggcc tcgacttcac ccgggtccag ttcacgcccg acctgctgcc
     5161 cgccgatctg ctcggttcga cggtctacga catgcagtcg ggccatttcg agttccgccg
     5221 cggaccgatc ttcaccaacc tgcttctcgc cgacgaaatc aaccggacac cgcccaagac
     5281 ccaagcagcg ctgttggagt cgatggccga gggacaggtc agcatcgacg gtgtcaccca
     5341 caagcttcct gccccgttca tcgtgctggc cacggacaac ccgatcgaat acgagggcac
     5401 ctatccgctg cccgaggcgc agctcgaccg gttctcgatc aggctcgaat tgcggtacct
     5461 gtccgagcag gaggaggcgt cgatgttgcg gcggcgcctc gaacgagggt cggcccagcc
     5521 gatcgtgcac caggtggttg atgggcacga ccttctcgcc atgcgtgaat ccgtggaaca
     5581 ggtgaccgtg cacgacgacg tgttgggata cgtggtgtcg ctggcgaccg cgacccgcca
     5641 acacccccaa gtagccgtcg gtgcgagtcc acgggccgaa ctcgacctca tgcaactcgc
     5701 ccgcgccagg gcgctactgc tcggcaggga ctacgtgatc cccgaggacg tcaagtacct
     5761 ggccgtgccc accatggcgc accggatcag cctgcggccg gagatgtggg tgcggcgcgt
     5821 gcatggctcc gacgtcgtcg aggaactgct gcggcggctg ccggtcccgc ggaccaaagg
     5881 ctgacgtcgt gatcgagacg cacagcaccg aagtcgaatt acgctggcga ccagcacctt
     5941 tgacgcgctc ggtggtgacg tgtgcggccg ccgcgctcgc ggtcgcggtg atcggtaggt
     6001 actggcagct ggtggcgttc gcggcgccgc tgatcggcgt actggtgtca atcggatggc
     6061 agcgcgaggt gtcgaaggtc catgtgcacg ctgagccagg cggccagcgc tgcttcgaat
     6121 ccgaggagac gcgggttagg gtgtcggcca ttgccgaaac ccccgatgtg gacgtgcatc
     6181 tcacgctcga ggccgtcgac gggatgcgcc tcgagaccgt cgagctccag tcgccgcagc
     6241 gtcagacggt ggccgtcgcc gccgacacgt ggggccggta tccgatcgtg gcccgcgtga
     6301 atgtcctcgc gcgcggcggc cttgtgctgg gttcaggcgc cgccgacgcc gccaacatct
     6361 acgtattccc gctggcgcca ccgcaatcca ccgcgatccc gcgcacagag ttgctcgacc
     6421 gtctcggtac ccacttgacc cgacacatcg gtcccggcgt cgagtacgcc gacgtccgcc
     6481 ggtacgtgcc gggcgaccag ttgcggacgg tcaactggcc ggtcagcgcg cggcgcggca
     6541 gcctgcacgt cacggaacgg ttgacagatc gtgccgccga tgtggtcgtc ctgatcgatg
     6601 gatatccgca accaccgggt ccggcgaccg aagccaccga ccgcgtcgtg cgcggtgcgg
     6661 tgcaggtcgt gcagagcgcg ctgcgcagcg gcgatcgcgc gggcatcgtc gcgctcgggg
     6721 accgccaccc gcgctggctc ggtgcggaca tcgggcgtcg gcagttttac cgcatcctcg
     6781 atgccgtgct caccgcgggc gaggacttcg agaccacgcc gggcacgttg gcgccgcagg
     6841 cggcgctccc tcctggcgcg attgtgatcg cgttctccac cctgctcgac accgagttcg
     6901 cgctggcgtt gatcgacctg cgcaagcgtg ggcataccgt cgtcgccgtc gacgtgctgg
     6961 agggcgcccc tttcgacgag gaatgcgacc cgctgatcgc ccggatgtgg tcgctgcagc
     7021 gctcgttcat gtaccgcgac atgcgcacga tcggcgtcga cgtggtgtcg tggcacggcg
     7081 atgccacgct cgatcaggca atgcaactgg tgcccgatca ccggcgctcg gcgcggcggc
     7141 gggcggcgct cagatgagca cccgaagacg accgcgagtg aggatcgcag cgaggaacga
     7201 gcgaggaccg gagcgagtgg gagtcgaggc atgagttgcc ctgtgtcaag ccgccctggg
     7261 ttggtcttgg ttgagcagtg tttggagggc gcggtgtttg gcggggtcgt aggcgacttc
     7321 gtcttgccag cagtgccaga tgacgtagag ccaggcgcgg gcgaggatgc gcacggcgtg
     7381 ggggtggtcg tgtccgcggg cgatggcgcg gttgtagatg tcggcggccc aggggttggc
     7441 gcgtcggctg tcgccggcga agtcggtgac ggcgtcgcgg agttgtttgt ctgcggccca
     7501 acggaatccg acgtgtttga ctttgccgga ttggcgggtg gagggcgcga cgccggccag
     7561 gcaggccaac gattcggggg tggggaagcg ggcgcggcag tccccgatct cgctgagcag
     7621 gcgcgccgcg cgcacggtgc ccgagcgcgg cagcgaggtg aagatctggc tgtcggcgtg
     7681 gcgggcgagt tgttgggcga tgtcgtcgtc gagggccttg atctgggcgg tcagggccga
     7741 cagtacggcc acgaaggcgg cggtggtggc ggtgtggtcg gttcccgcgg tgccgcgcgg
     7801 tgcactagtg agccgtcggt gcagcaccgc agggtcggtg cggccgctgt agccgacgct
     7861 ggccagccag gccgccagcc ggttgatcga gagccagtcg gcgcggtctt ggcagtcgaa
     7921 gcgagtcaga aacgccaggc tgatcgggct gtcgagggcg gcgaacaaac cgaccgcgcc
     7981 ggggaacacg atctgcaggt gcgcgcgcag ctggttggcc accgcgacgc ggtgggcgac
     8041 gaggtctttg cgggcgcggg aggtctggcg cagggtgatg gtggccgggg tgtcgggcaa
     8101 cagcggagcc agccgtgctc ggtcggtgcg cagggtgtcg gcgagcacga acgcgtcgaa
     8161 ccggtcgtct ttgttgcccg ccgagccgta gcgtccgcgc aggttcttga cttggttggg
     8221 gctgatcacc acgaccgtga tcccggcgtc aagcagggtg tccacgaccg ggccgtcggg
     8281 gcgttcgatc gcgacctcgg ccacgccggc gcgctcaagc acgacgagca actcgcgcag
     8341 tccggcagcg ttgtgctcga cggtggatcg cacgatttcg cggccgcggt catcgacgat
     8401 cgacaccgcg tgatcatcac gcgcccagtc gattccgccg gtgacgccgt tggcgggttc
     8461 cgtcggcaag gtatttcggg cagacttcaa gacagcctcc tcgctgctag tcccagtggg
     8521 gaggcacctt cgtgtggtac cggggcacga ctgctcgttc gctcactgtt cggcgctcgc
     8581 aggcgctcag ccctgtcgac ggtcggcgtg ccccgggcga acgctgggtt ccgcagaact
     8641 catgctggac ataactgctg tcgagcgagc agggcgataa cccagcgcca cctcaggtgc
     8701 atcggcaacc catccagaat caccgacaca aggatggtgc accagtgagc accgctgcac
     8761 cggtcacccg cgccgtctcc acggcgttcg gattggtgat ggtggcgtcg gccgccgtgc
     8821 aggcggatag agcgggtttg atcgcggccg tcgtcgcggc ggctgcggtg ttggcaggca
     8881 gtgtatttcg gcctgctgcc acggtcgccg tgctggtcac ggtggtggtg atcgtgctga
     8941 ccgatgcgcc acccgtgttg accgcgacgg ccgggctgtg tgccgccgcc tatctggtgt
     9001 tgcgacatac cgccgcggtg accgcaccga cagtgcttgg cgccgtgggg tttaccgcgg
     9061 ccgggctggc agcggcatcg gtttcgatcg agatgccgtg ggtgccgctg ctggcccccc
     9121 tggcggtgtt ggttgtctac gttctggtca ctcgcccgtt ctgggcgaca tagcggccgg
     9181 gtattcgtcg tggcggcgcc accacgcata cggtggcgtt tgtggatatc cgtcgggtga
     9241 gtcctcccac tcctcctgac ggccgagcgc agtgatgtcg agatacgccc atgtgctgcc
     9301 catcgcttcg tcgccgcggt tgtcgatgaa atacgtgcgg aagatctggc cgtcgtcacg
     9361 caggaacgcg ttggtgccgt gccactcgtc gacaccgaag tcggcgtcga acgaatccgt
     9421 cagcgagtac cagggaatct cccagtccat ccgcttcttc agccgcgcga tcgcgtcccg
     9481 aggagcacgg gagacgaaca ccagcgtggt gtcacgcgcg ttgaggtgcg ccaggtgacc
     9541 cacttggtcg gccagcatcg aacaccccgg gcaaccgcgg tcgggccagc cgtccacatc
     9601 gggctggtag aagaagcggt acacgatgag ctggcgcctg ccgtcgaaca gatcgagcag
     9661 gctggccgga ccgcccggcc cgtcgaacag ataatccttc tcgaccagca tcctgggcat
     9721 cctgcggcgc tgtgccgcca gggcatcgcg ggcgcgggtg agttccttct ccttcaccag
     9781 caattcctgg cgcgctgtgt cccattcctg gggtgagacg atcggcggga aatccatcgc
     9841 cgtacctcct tcgaatcggt ccgatatata gacacccgca acgggcgaaa atcatcgccg
     9901 ctatcttctt tccagatggt gacctgcgct tccgtaagcg aaatgctcag cgggacgctt
     9961 actttcggga gagccgacat gccaaacgat gtgcgcaagg cggtgaccgg tgcatcgatc
    10021 ggcaacgccg tggagtggtt cgacttcgcc atctacgggt tcttggccac gttcatcgca
    10081 gccaacttct ttccgtccgg aaacgagacc gccgccctgc tgaacacctt cgcgatcttc
    10141 gcggcggcgt tcttcatgcg gcccctcggc gggttcgtat tcggaccgct cggcgaccgg
    10201 atcggccggc aacgcgtgct ggccctcgtc atcctgctga tggccgccgc gacggtggcg
    10261 atcggcctgc tgccgaccta cgccacgatc ggcgtcgccg ctccactgct gctgctgttc
    10321 ctgcgctgcc tgcaggggtt ctccgcgggc ggcgaatacg gcggcggcgc agtgtatctc
    10381 gccgagttcg cctccgagaa gcatcgcggc ctgaccgtca cgttcatggt gtggtccggg
    10441 gtgctcggct tcctgctcgg ctcggtgacg gtaacgctgt tgcaggcgct gctgcccgcc
    10501 ggcgcgatgg agagctacgg ctggcgcatc ccgttcctga tcgccgggcc gctgggcctc
    10561 gtcggcctct acatccgcct acgcctcgat gacaccccgc agttcgcaga gctgagcaag
    10621 gccgagcagg tcgccaagtc gccgcttcgc gaggcaatca ccaccgcgtc gcggccgatc
    10681 ctgcaggtga tcgggctgat gatcatcttc aacatcggct actacgtggt gttcaccttc
    10741 ctgcctagct acttcatcaa aacgctgcac ttctcgaaga ccaacgcgtt cgtgtcgatc
    10801 accctggcca gcctggtggc gctgatcctg atcctgccgc tggccgcgct gtccgaccgc
    10861 gtgggtcgca gggccatgct gatcacgggc gcgctggcct tcgcgatcct cggctaccca
    10921 ctgttcctgc tgctgaactc ggggtccctg gcggccgcga tcgcggcgca ctgcggcctg
    10981 gcggcgatcg aggccatcta cgtgtccact gcggtggccg ccggggtcga gctgttcgcc
    11041 acgcgggtgc gctacagcgg cttctcgatc ggctacaacg tctgcgtcgc
//
LOCUS       DASRJA010000271         3733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_672980,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000271 DASRJA010000000
VERSION     DASRJA010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672980"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2145
                     /locus_tag="VFC01_12105"
     CDS             <1..2145
                     /locus_tag="VFC01_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZN80415.1"
                     /translation="QWIDTFLRVLIGSAVVLVPIVLAAVAVVLMRTEPDPDARPRLIL
                     GAAMITLPTLGLWHLWAGSPQDPTARQHAAGFLGFAIGGPLSDGLTAWIAAPLLFIGA
                     LFGLLLVTGTTIREVPSTVQRMFSVRAFHGDLYDEEYDDEQPYTEEAEDFSDGYYDNP
                     SAYNDDEAQAWPTGTPMENYPLDDEAPTVPEPTAKTRRKKPAKKLTLSLDRVVDGPYT
                     LPPLELLKAGDPPKRRTAANDRMAEAITEVLDQFKVNASVTGCTRGPTVTRYEVELGP
                     GVKVEKITALHRNIAYAVATESVRMLAPIPGKSAVGIEVPNVDREMVRLADVLTDPST
                     RGDHHPLVIALGKDIEGEYISWNLAKMPHLLVAGSTGSGKSSFVNSMLVSLLARATPE
                     EVRMILIDPKMVELTPYEGIPHLITPIVTQPKKAAAALAWLVEEMEQRYQDMQASRVR
                     HIDDFNKKVRSGEITTPLGSEREYRPYPYIVAIVDELADLMMTAPRDVEDAVVRITQK
                     ARAAGIHLVLATQRPSVDVVTGLIKTNVPSRLAFATSSLTDSRVILDQQGAEKLIGMG
                     DGLFIPMGANKAIRLQGAYVSDDEIHAVVQACKDQAEPEYTEGVTKAKTNSERTDVDP
                     DIGDDMDVFLQAVELVVSSQFGSTSMLQRKLRVGFAKAGRLMDLMETRGIVGPSEGSK
                     AREVLVKPDELAGTLALIRGGSDANGGDDEVD"
     gene            complement(2142..2390)
                     /locus_tag="VFC01_12110"
     CDS             complement(2142..2390)
                     /locus_tag="VFC01_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80416.1"
                     /translation="MRRRIVICVVAAFAAAAVLIAPAPAQADPPQCDNGPIVLDGCTS
                     PGVCTAVIDNQCVGVQPPLLPAPPPVKVGIEGGVGVGA"
     gene            complement(2408..2917)
                     /locus_tag="VFC01_12115"
     CDS             complement(2408..2917)
                     /locus_tag="VFC01_12115"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HZN80417.1"
                     /translation="MSGASERVVVRRARTSDIPAMKALVDIYSGKILLEKNLVTLYEA
                     VQEFWIAELHGELIGCGALHVLWSDLGEIRTIAVHPKVRGQGVGHAIVDRLLDVARQL
                     HLERIFVLTFEVEFFSRHGFEEIEGTPVTAEVYEEMCRSYDTGVAEFLDLSYVKPNIL
                     GNTRMLLTL"
     gene            2960..3556
                     /gene="pgsA"
                     /locus_tag="VFC01_12120"
     CDS             2960..3556
                     /gene="pgsA"
                     /locus_tag="VFC01_12120"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZN80418.1"
                     /translation="MSGQPHIDPVVPRARVANIANVLTGVRMVLVPVFLAVLFVGDGH
                     ETFWRIAAFVVFAVAVITDRFDGALARSYGMVTEFGKLADPIADKALIGAALIGLSML
                     GDLPWWVTVVIMVREIGITVLRFVVLRHGVIPASRGGKLKTLVQAVAIGLFVLPLSGL
                     WLTGAWVVMWAAVVLTVLTGVDYVVSAVRDSRGRPAGH"
     gene            3534..>3733
                     /locus_tag="VFC01_12125"
     CDS             3534..>3733
                     /locus_tag="VFC01_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HZN80419.1"
                     /translation="MDDPLVTEDARALIADLTVRRQSVATAESLTAGLLAATLAGVAG
                     ASVVLRGGLVTYIEDTKIALAGV"
BASE COUNT          642 a         1202 c         1243 g          646 t
ORIGIN      
        1 caatggatcg acacgttcct tcgggtgctg atcgggtcgg ctgtcgtcct cgtgcccatc
       61 gtgctcgccg cggtcgcggt ggtgctgatg cgcaccgagc ctgaccctga tgcacgtccg
      121 cggctcatcc tcggtgcggc gatgatcacg ctgcccactc tcggcctgtg gcatctgtgg
      181 gcgggttcgc cgcaagatcc caccgcgcgt cagcacgccg cgggattcct gggcttcgcc
      241 atcggcggcc cgctgtccga cggcctgacc gcctggatcg ccgcgccgct gctgttcatc
      301 ggcgcgctgt tcggcctgct tttggtgacc ggcacgacga tccgcgaagt gccgtcgacg
      361 gtgcagcgca tgttctccgt gcgcgccttc cacggtgatt tgtacgacga ggagtacgac
      421 gacgagcagc cgtacaccga agaggccgaa gacttctccg acggttacta cgacaacccg
      481 tccgcgtaca acgacgacga ggcgcaggcg tggcccaccg gcaccccgat ggagaattat
      541 ccgctcgacg acgaggcgcc caccgttccg gagccgaccg ccaagacacg ccgcaagaag
      601 ccagccaaga agctgacgct gtcattggat cgagtcgtcg acggcccata cacgttgccg
      661 ccgctggagc tgctgaaggc gggcgatccg ccgaaacggc gcaccgcggc caatgaccgg
      721 atggcggagg ccatcaccga ggtgctggac caattcaagg tgaacgcatc ggttaccggc
      781 tgcacccgcg ggccgacggt cacacgctac gaggtcgaac tcggccctgg cgtcaaggtc
      841 gagaaaatca ctgcgctgca ccgcaatatc gcctatgcgg tggccactga gagcgtccgg
      901 atgctggcgc cgatccccgg caagtccgcc gtcggcatcg aggtgcctaa cgtcgaccgg
      961 gaaatggtgc gtctcgcaga cgtgctgacc gatccgtcga cgcgtggcga ccaccatccc
     1021 ctcgtgattg cgttgggtaa agatattgag ggcgaataca tctcgtggaa cctcgcgaag
     1081 atgccgcacc tattggtcgc cggctcaacg ggttcgggca agtccagctt cgtcaactcg
     1141 atgctggtat cgctcctcgc gcgggccact ccggaggagg tcaggatgat cctgatcgac
     1201 ccgaagatgg tggaactcac gccgtacgaa ggcattccgc acctcatcac gccgatcgtc
     1261 acgcagccca agaaggcggc cgccgcgttg gcatggctgg ttgaggagat ggagcagcgc
     1321 taccaggaca tgcaggccag cagggtgcgt cacatcgacg acttcaacaa gaaggtgcga
     1381 tcgggtgaga tcaccacgcc gctgggcagc gagcgcgagt accgtccgta cccctacatc
     1441 gtggcgatcg tcgacgagct cgccgacctg atgatgaccg cgccgcgcga tgtggaggac
     1501 gcggtcgtcc gcatcaccca gaaggcacgc gccgcgggta ttcacctcgt gctcgccacg
     1561 cagcggccgt cggtggacgt cgtcaccggt ctgatcaaga ccaacgtgcc gtcgcggctg
     1621 gccttcgcga cgtcgtcgtt gaccgacagc cgcgtcatcc tcgatcagca gggcgccgag
     1681 aagctgatcg gcatgggcga cggcctgttc atcccgatgg gcgccaacaa ggcgatccgg
     1741 ctgcagggtg catacgtttc cgacgacgag atccacgccg tggtacaggc atgtaaggac
     1801 caggcggagc ccgagtacac cgagggcgtc accaaggcca agaccaacag cgagcgcacc
     1861 gacgtcgacc ccgacatcgg cgacgacatg gacgtcttcc tgcaggccgt cgagctggtg
     1921 gtgtccagcc agttcggctc gacgtccatg ctgcagcgca agttgcgggt cggcttcgcc
     1981 aaggccggcc ggttgatgga cctgatggaa acccgcggca tcgtcggtcc cagcgagggt
     2041 tccaaggcgc gcgaggtgct ggtcaagccc gacgagctgg cggggacgct ggccctgatc
     2101 cgcggtggtt cggatgctaa cggcggagac gacgaggtcg actaggcccc gacgccgaca
     2161 ccgccttcga tgccgacctt gacgggcggc ggcgccggaa gcaggggcgg ctggactccg
     2221 acgcactgat tgtcgatcac ggccgtgcag actcctgggc tcgtgcagcc atcgaggacg
     2281 atcgggccgt tgtcgcattg cggtgggtcg gcctgcgccg gcgccggcgc gatcagaacc
     2341 gccgccgctg cgaacgccgc gacgacgcag atgacaatcc gacgccgcac gttgctaacg
     2401 gtacgcatca gagcgtcaac aacattcggg tattgccgag aatgttcggc ttcacatagg
     2461 acaggtcgag gaactctgcg acaccggtgt cgtaggagcg gcacatctcc tcgtagacct
     2521 ccgcggtgac cggcgtgccc tcgatctcct cgaagccgtg ccgcgagaag aactcgactt
     2581 cgaaggtgag cacgaagatc cgctccaggt gcagttgcct ggccacgtcc agcaatcgat
     2641 ccacgatcgc gtgtccgacg ccctggcccc tgaccttggg gtgtacggcg atagtgcgga
     2701 tctcgccgag gtcggaccac aacacgtgca gcgcgccgca gccgatcagc tcaccgtgga
     2761 gttcggcaat ccagaactcc tggaccgcct cgtagagcgt cacgagattt ttctccagca
     2821 agatcttgcc ggagtagatg tcgacgagcg ctttcatggc aggaatgtcg gacgtgcggg
     2881 cacgccggac gacaacccgc tcggacgctc cgctcacacg tgcgagggta tcggcttcgg
     2941 caaccgatat tctgttgcgg tgtcgggcca acctcatatc gatccggtgg ttccgcgcgc
     3001 ccgcgtggcc aacatcgcga acgtgctcac cggtgtgcga atggtgttgg tccccgtgtt
     3061 cctcgctgtg ctgttcgttg gcgacggcca tgaaacattc tggcggatag ccgcattcgt
     3121 ggtcttcgcg gtcgctgtga tcactgaccg gttcgatggc gcgctggcgc gcagctacgg
     3181 gatggtgacc gaattcggca agctggccga tccgatcgcc gacaaggccc tcatcggagc
     3241 ggctctgatc gggctgtcga tgctcggtga cctgccctgg tgggtgacgg tggtgatcat
     3301 ggtccgtgag atcggcatca ccgttttgag attcgtggtg ctgcgccatg gtgtcatccc
     3361 ggccagccga ggcggcaaac tgaagacgct cgtccaggcg gtcgcgatcg gcctgttcgt
     3421 gctgccgctc tcggggttgt ggctgaccgg ggcctgggtg gtgatgtggg ctgccgtcgt
     3481 gctgacggtg ctcaccggcg tggactatgt cgtctctgcg gtcagggatt cgcgtggacg
     3541 acccgctggt cactgaggac gctcgcgcgc tcatcgccga cctcaccgtg cgcagacaga
     3601 gtgtcgcgac cgccgagtcg ctgacggccg gcctgctggc cgcgaccctg gccggcgtgg
     3661 ccggcgccag tgtggtgctc cgcggcggtc tggtcaccta catcgaggac accaagatcg
     3721 cgctggccgg ggt
//
LOCUS       DASRJA010000272         4380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3643127,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000272 DASRJA010000000
VERSION     DASRJA010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3643127"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VFC01_12130"
     CDS             complement(<1..409)
                     /locus_tag="VFC01_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80420.1"
                     /translation="MITPVPTTSALYPVRLRGDLQRDVAYAYWAGPHAEIVKRLPHVV
                     EYNQYHFSATDHGYWPATPTVGTTVPSAWPLDGLSEVRMSSMAAALRIPLHMREVFLD
                     EQNVFEHCLGHLTGPGGGRWWTAGHDDSAGHRTV"
     gene            complement(409..1677)
                     /locus_tag="VFC01_12135"
     CDS             complement(409..1677)
                     /locus_tag="VFC01_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HZN80421.1"
                     /translation="MTTSSASPLLQPLQIGPLTVPGRLYKSATSETRATDEGYVTDEL
                     LQFYEPMVRAGTPLIVTGNLFVSLQSKSAGRQAGIDSDDKKPGLRDWVQLAHSGGSLL
                     VAQLNHGGRQMVESSDPGKATVSASSVREPLYGTKPRPLRVDEIPALVDTFAAAAERA
                     QDVGFDGVQIHAAHGYLLGQFLTPHTNRRNDEYGGSLDNRMRLLREVFRAIRARVGDD
                     FPILLKMNGTDMLPLRKGATTAELVRVAKAMADEGIDAIEISRGHYESWPGMILGHYR
                     GFISGQVKQGSGTKMSRRRRMTMLALAPIIELAAEYLAPGGEGFNLPQAEQFTAQLDI
                     PVICVGGFHTTKGMESAIETGRCDAVSAARAFIADPYLFKNVQMPTPGRPVCGYCNGC
                     IARFGGKPIDCYNDEIRAQKDLMLHRVEGF"
     gene            complement(1692..2693)
                     /locus_tag="VFC01_12140"
     CDS             complement(1692..2693)
                     /locus_tag="VFC01_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN80422.1"
                     /translation="MPDIFGNVHLGYVVIETEKFGDWRRFGRDAIGMHVDEALPDVMR
                     FRLDDNKCRFLLQRGPAEDATALGWQLDDHSTFDEILARVTRHGVPVTEGTPEEAALR
                     GVERLVRFPGPNGLAQEIFTRARTSDTPLDMRVGGGFVTGEFGMGHVAITTKKPHQMR
                     GYYNRVFDARLSDYIDETISGVKFKIRFLRVNERHHSVAIAAVNRLPVNPIRTRIQHL
                     NIQVADLDDMTASHQRVKELGFDMALSVGQHTNDKELSYYAMTPSGFEWEVGWNPIVV
                     DEKTWETTTYQGISIWGHTPEGQTIIDKLAMFKVGVQSLVHREDSVPALAGAGIPDN"
     gene            complement(2686..3552)
                     /locus_tag="VFC01_12145"
     CDS             complement(2686..3552)
                     /locus_tag="VFC01_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN80423.1"
                     /translation="MTTANLTEHTVTVDGKPIFVAETGSGPAVVMLHGGGPGASGVSN
                     YSRNIDALAQHFRVIVPDMPGYGRSMKGVDRDDPFGYLADMIRGLLDELGIDTAHLIG
                     NSYGGAAALRLALDTPHRVDKLVLMGPGGIGTTRGVPTAGLKSLLSYYGGDGPSREKL
                     ETFIRTYLVYDGASVPDELIDVRYRASIDPDVVGNPPLRRPSGPRALWRMDLTRDSRL
                     KTLQTPTLVLWGRDDKVNRPAGGPMLLNTMPNAELVMTSHTGHWMQWERAEFFNQLVS
                     EFLGAESKLAHA"
     gene            complement(3549..>4380)
                     /locus_tag="VFC01_12150"
     CDS             complement(3549..>4380)
                     /locus_tag="VFC01_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZN80424.1"
                     /translation="RTDEDDRHLLRDEEIWKVLADQGITAEHVEILRAVIYSHHVRVA
                     DRWRVGRVFLAGDAAHAMPPWIGQGMSAGVRDVANLCWKLAAVLRGQAPDSLLDSYQA
                     ERKPHVTEVTRRACFVGRIITERNRVVATMRNHVFRALTRVPGVDALLLKMTWIPDAR
                     YEDGFFATGRHPAVGWQIPQPWVTDDTDTRVRLDDVLDGQWAVLHTGATPAGAQAWTQ
                     LGAKTIRISDLTLVRWLQHKKAAAVVVRPDGFIYAASQSGQPLPAPPAITPIRTGVPA
                     "
BASE COUNT          745 a         1341 c         1431 g          863 t
ORIGIN      
        1 gcaccgtgcg atgaccggcc gagtcgtcgt gaccagccgt ccaccagcgg ccgccgccgg
       61 gtccggtcag gtgaccaagg caatgctcga aaacgttctg ttcgtcgagg aagacctcac
      121 gcatgtgcaa cggtattcgc aatgcggcag ccatactcga catccgcacc tcggagagcc
      181 catcgagcgg ccaggccgac ggaaccgtgg ttccgaccgt gggggtggca ggccagtaac
      241 cgtggtcggt cgcggagaaa tggtattggt tgtactccac gacatgcgga agacgtttga
      301 cgatctcggc gtgcggtccg gcccagtatg cgtacgcaac atcgcgctgc agatctcccc
      361 tcagtcgcac ggggtagaga gccgaggtgg tcgggacggg cgtgatcatc aaaacccctc
      421 aacacgatga agcatgaggt ccttttgggc acggatctca tcgttgtagc agtcgatcgg
      481 ttttccgccg aagcgggcga tgcaaccgtt gcagtatccg cacaccggcc ttccgggcgt
      541 cggcatctgt acgttcttga acaggtatgg atcggcgatg aaagcccgcg ccgctgacac
      601 ggcatcgcat cgacccgttt cgatcgccga ttccatacct ttcgttgtat gaaatccgcc
      661 gacgcagatg accgggatat cgagttgggc ggtgaattgt tcggcttgtg ggagattgaa
      721 tccttcacct ccaggggcca gatattcggc tgcgagttcg atgatgggtg ccagggcgag
      781 catggtcatg cgtcgccgcc gcgacatctt ggtgcccgag ccctgtttga cctgtccgct
      841 gatgaatccc ctgtagtgcc cgagaatcat tccgggccag gattcgtagt gaccgcgtga
      901 gatttcgatt gcatcgatac cttcgtcggc catcgccttc gcgacgcgca cgagttcagc
      961 cgttgtcgcc cctttgcgca gagggagcat gtcggtgccg ttcattttca agaggattgg
     1021 gaagtcgtcc ccgacgcgag cgcgtatggc ccggaacacc tcgcggagca gtcgcatccg
     1081 gttgtcgagg ctgccgccgt attcatcgtt gcgacgattg gtgtgcgggg tgaggaactg
     1141 gcccaggagg tatccgtgtg cggcgtggat ttgcacaccg tcgaatccga catcttgtgc
     1201 gcgctcagcg gcggcggcga atgtgtcaac caatgccggt atctcgtcca cgcgaagtgg
     1261 gcggggcttg gtcccgtata gaggttcgcg aaccgacgac gccgacacag tggccttgcc
     1321 tggatcagac gattcgacca tctggcggcc gccatggttg agctgcgcga caagaaggct
     1381 gccaccgctg tgcgcaagct gtacccaatc acgcaggccg ggcttcttgt cgtcggagtc
     1441 gatgcccgcc tgccgacccg ccgacttgga ctgcaacgac acgaaaaggt ttccggtcac
     1501 gatcaaagga gtccccgcac gcaccattgg ctcgtagaac tgcagcaact cgtcggtgac
     1561 gtatccctcg tccgtcgccc gagtctcgct tgtagcagat ttgtacagcc gcccgggaac
     1621 cgtcaaaggc ccaatctgca gcggctgcaa cagcggactc gctgaactcg tagtcattcg
     1681 tcttgtctcc gctagttatc cgggattcct gcgccggcca acgccggaac gctgtcttca
     1741 cggtgaacca gcgactggac gccgaccttg aacatcgcaa gcttgtcgat gattgtctgt
     1801 ccttccggcg tgtgccccca gatgctgatg ccctgatatg tggttgtttc ccaagtcttt
     1861 tcatcgacca cgatgggatt ccagccaact tcccattcaa agcctgacgg cgtcatcgcg
     1921 tagtaagaca gctctttgtc gttggtgtgc tgaccgacgc tcagcgccat gtcgaatccg
     1981 agttccttga cccgctgatg ggaagcggtc atgtcgtcga ggtcggccac ctgaatgttc
     2041 aggtgctgga tgcgagtccg gatcgggttg acgggcagcc ggttcaccgc ggcgatcgcc
     2101 accgagtgat ggcgctcatt gacccgcagg aaccggatct tgaacttgac gccgctgatc
     2161 gtttcgtcga tgtagtcgct caggcgcgcg tcaaacaccc tgttgtagta accccgcatc
     2221 tgatgcggct tcttggtcgt gatcgcgacg tgtcccatcc cgaattcccc agtgacaaag
     2281 ccgccgccga cgcgcatgtc cagtggcgta tcgctggtgc gggcgcgggt gaagatctcc
     2341 tgcgccagcc cattcggtcc cgggaacctc acgaggcgct cgactcctcg caatgccgct
     2401 tcttctggag tcccctcggt gacgggcacg ccgtgccggg tgacccgcgc gaggatttcg
     2461 tcgaaggtgc tgtggtcgtc gagttgccac ccgagtgcgg tggcgtcttc ggccggtccg
     2521 cgctgcagca ggaaccggca tttgttgtcg tccaagcgga atcgcatgac gtcgggcagc
     2581 gcctcgtcga cgtgcatgcc gatggcgtcg cggccgaagc gtcgccagtc gccgaatttc
     2641 tccgtctcga tgacgacgta gccgaggtgg acgttgccga agatgtcagg catgagcgag
     2701 cttcgattcg gcaccgagga attctgagac gagttgattg aagaactcgg cacgttccca
     2761 ctgcatccag tggccggtat gtgacgtcat caccagttcg gcgttgggca tcgtgttgag
     2821 cagcatcggt ccgccggccg gtcggttgac cttgtcgtcg cggccccaca gcaccagcgt
     2881 tggtgtctgc agcgtcttga gccgtgagtc gcgggtgagg tccatccgcc acagcgcgcg
     2941 gggacccgac gggcggcgca gcggcgggtt gcccaccacg tccggatcga tcgaggcgcg
     3001 atagcgcaca tcgatcagct cgtcgggcac cgaggcgccg tcgtagacaa ggtatgtgcg
     3061 aatgaaggtt tcgagcttct cgcggctcgg tccatcgcca ccgtagtagg acagcaggct
     3121 tttcaggccg gcggtcggga cgccccgcgt cgtcccgatc ccgccgggtc ccatcagcac
     3181 cagcttgtca acgcggtgcg gagtatcgag ggcgagacga agtgcggccg caccgccata
     3241 cgagttgccg atcaagtggg cggtatcgat accgagttcg tcgagcagac cgcggatcat
     3301 gtcggcgagg tagccgaacg ggtcgtcccg atccacgccc ttcatcgagc ggccgtagcc
     3361 gggcatgtcc ggcacgatga cccggaagtg ttgtgcgagc gcgtcgatgt tgcgggagta
     3421 gttggacacc cccgatgcac cgggtccccc gccgtgcaac atgaccacgg ctgggccgct
     3481 gccggtttcg gcgacaaaga tcggttttcc gtcgacggtg acagtgtgtt cggtcagatt
     3541 ggcggtggtc atgcgggtac tccggttcta atcggcgtga tggcgggagg ggcgggtagc
     3601 ggctgcccgg attggcttgc ggcgtagatg aatccatcgg gtcggacgac gacggcggct
     3661 gccttcttgt gctgcagcca gcggacgaga gtgagatcgc tgatccgaat cgtcttggcg
     3721 cccagctggg tccaggcctg cgcgccggcc ggagttgcgc cggtgtgcag gacagcccac
     3781 tgtccgtcga ggacgtcgtc gaggcggacc ctggtgtcgg tgtcatccgt cacccacggt
     3841 tgcggaatct gccaaccgac ggcggggtgg cgccccgtgg cgaagaagcc gtcctcgtat
     3901 cgggcgtcgg gaatccacgt catcttcagg agcagcgcgt ccacaccggg cactcgcgtc
     3961 agcgcacgga acacgtgatt tcgcattgtg gcgacgacgc ggttgcgctc ggtgatgatc
     4021 cggccgacaa agcatgcgcg gcgggtgacc tcggtgacgt gcggtttgcg ctcggcttgg
     4081 taggagtcga gcagcgagtc cggtgcctgc ccgcggagca cggcggcgag tttccagcac
     4141 aggttggcca cgtcgcgtac ccctgcggac atcccctggc cgatccaggg cggcatggcg
     4201 tgcgcggcgt cgccggccag gaagacccgc cccacccgcc accggtcggc gacgcggacg
     4261 tggtggctgt agatcaccgc acgcaggatc tcgacgtgct ctgcagttat gccctgatcc
     4321 gccaacacct tccagatctc ctcgtcgcgc aggaggtggc gatcatcctc gtccgtgcga
//
LOCUS       DASRJA010000273         6744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2140733,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000273 DASRJA010000000
VERSION     DASRJA010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140733"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1830)
                     /gene="fadD6"
                     /locus_tag="VFC01_12155"
     CDS             complement(70..1830)
                     /gene="fadD6"
                     /locus_tag="VFC01_12155"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase FadD6"
                     /protein_id="HZN80425.1"
                     /translation="MSGDTVGLTDIAVRLPALAMDAPVVLRGVLTAFLARRSAKRSIG
                     RVFQDRAARYGDHVFIRFDDEAWTYRDANHTVNRYAAVLAERGVARGDVVGIMMRNSP
                     RTVLLMLAVVKLGAIAGMLNHRQHGNMLAHSVGLLRAKVLIGEDDLLASVDESGDISD
                     TLTVDELERLAAGAPATNPEVTSSVLAKDRAFYIFTSGTTGAPKASVMTHYRWLRAMG
                     GSGALGMRLKSSDTLYCCLPLYHNNALTVAVSAVISAGATLALGRSFSASRFWDEVVR
                     YDATAFVYIGEVCRYLLNQPEKDTDRTNKVRVIAGNGLRPEIWDEFTTRFEIPRVFEF
                     YGASEGNTGFVNIFHIPKTAGLCPTTVAFAEYDPDTGDPVRDANGRVRKVRRGQPGLL
                     LSKVTKAQPFDGYTDPEASEKKLVRNAFRDGDVWLNSGDLLRPQGWRHAVFVDRLGDT
                     FRWKGENVATTDVEAAVAADPTIEAVTVFGVEVPGADGRAGMAAVVLNEDKEFDGASL
                     ANTVYDHLPGYAVPLFVRVVDSLEHTATLKTKKVDLREQGYGPDIDDPLYVLNGRDEG
                     YVQYYDEYPDDVAAGDRPAS"
     gene            complement(1836..3266)
                     /locus_tag="VFC01_12160"
     CDS             complement(1836..3266)
                     /locus_tag="VFC01_12160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZN80426.1"
                     /translation="MKRLSSVDAAFWFAETPGWHMHVGGLVICDPSNAADFSFDAVRE
                     LLASRLPEVPQLRYRVAGAPLGLDRPWLVEDAELDIDFHIRRIAVPPPGGRKELDDLV
                     GRLMSYKLDRARPLWELWFIEGVENGRVAILTKMHHALIDGVSGAGLSEILLDATPEP
                     RPPAAEPKESLVGVGMPRFELRALGGMFNLAVMTPYRVLRMVEQTVRQQLAVFGLANK
                     PPRYFDAPTTRFNATISPHRRVSGSRVPLDRVKTVKDAFGVKVNDVVLAMVCGALRRY
                     LLDRGELPDRSLLAQIPVSTRGEDTGVGNQITSTTVSLPTENSDPSDRIRAIHASAQG
                     AKAMAKALTAHQIMGLTEITPPGLLALAARAYTASRLGENVAPINLVVSNVPGPDFPI
                     YMAGAVLETLVPIGPLVMDVGLNITCFSYRGSVDFGFVTTPEIANDIDELADGIEPAL
                     RELEHAAGLSSPRQTRRRRSQRSPSG"
     gene            complement(3608..3690)
                     /locus_tag="VFC01_12165"
     tRNA            complement(3608..3690)
                     /locus_tag="VFC01_12165"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3654..3656),aa:Tyr,seq:gta)
     gene            3787..4557
                     /locus_tag="VFC01_12170"
     CDS             3787..4557
                     /locus_tag="VFC01_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HZN80427.1"
                     /translation="MAIARACPTLLVVVVIAGCGGSDPQPTPSSHSSSPPTSATAQTP
                     PSSSQPSAAVSPVSQAASAAGFVDVRTIVPDAVVDLRYATPNNFVGVPLYPTDARCLV
                     HESMAPGLTSAAAVLRQQGEALVFWDCYRPHDVQVRMFEAVPNPAWVARPGSYARSHE
                     AGRSVDVTIARAQQDCPPPRQVGKFCLVEMGTDFDDFSPSATAFATDGVSAEAQANRA
                     RLRDAMGSGGLTVYTGEWWHFDGPGADVERPILDVPVN"
     gene            complement(4558..6060)
                     /locus_tag="VFC01_12175"
     CDS             complement(4558..6060)
                     /locus_tag="VFC01_12175"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN80428.1"
                     /translation="MDGTPFGRYEFVELLGRGGMGEVWRAHDTATDRIVAIKVLPPEL
                     ANDAVFQQRFRREARAAAQLNEPHVIPIHGYGEIDGRLYVDMRLIEGRDLETILRAGP
                     LAPARAVTIVEQIAQALHAAHRVRLVHRDVKPSNILVNEFDFAYLIDFGIARGADETR
                     LTNTAAMIGTYQYMAPERIDGGDVDARADIYALACVLYECLTGAQPYPGDSVPSQIAG
                     HMLRPPPRPSTARRGVPANFDAVIAKGMAKDPNERYRTTLELAKAARSAVTAPMPQPP
                     RPPPERPAPATVTSAYPPSAAPVVPPPPPPPAPSPPAKGARHGTGRAAKVALGLAAVL
                     ALAVVAVMVGAFVRSTSDGGDTTESTRTSSRATSVPIADEPLMTPSTLPGTPTRTTSA
                     FPTSLVGKWSGPVSGDQSGFDVVADITADKPLTANVTYPQLNCAGTWQENGPAGNGVR
                     FITEKITQGTCVPAEVTLTPQDDGTVLYKSVYFSASQQRDFTIHATMRRS"
     gene            6246..6737
                     /locus_tag="VFC01_12180"
     CDS             6246..6737
                     /locus_tag="VFC01_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HZN80429.1"
                     /translation="MADSSFDVVSKVDRQEVDNALNQAAKELATRFDFRGTDTTIAWK
                     GEEVIEIVSSTEERAKAAVDVFKEKLIRRDISMKAFDAGDPQASGKTYKVSGTIKQGI
                     SSESAKKITKLIRDDGPKGVKAQIQGDEIRVSSKKRDDLQSVIALLKGADLDVALQFV
                     NYR"
BASE COUNT         1197 a         2237 c         2154 g         1156 t
ORIGIN      
        1 gtgacacttg tatgctcgct gtcctgcatc tttcctccac ttcgccgtca cccagatctc
       61 ggggcgattt cagctcgcag gccggtcgcc ggccgcgaca tcgtcggggt actcgtcgta
      121 gtactggacg tagccttcgt cgcggccatt gagcacgtag agcggatcgt cgatgtcggg
      181 cccgtatccc tgctcgcgca gatcgacctt cttggtcttc aacgtcgcgg tgtgctccag
      241 cgaatcgacg acccgcacga acaacggcac cgcgtagccg ggcaaatggt cgtacacggt
      301 attggccagc gaagcgccgt cgaattcctt gtcttcgttc agcaccacgg ccgccatgcc
      361 ggcgcgcccg tcggcgccgg gcacctcaac cccgaaaacc gtcacggctt cgatggtcgg
      421 gtcggccgcc acggcagcct cgacgtccgt cgtcgccacg ttctcgccct tccaccggaa
      481 cgtgtcaccg agccggtcga cgaacacggc atggcgccaa ccctgcggac gcagcaggtc
      541 accactgttc agccaaacgt cgccgtctcg aaatgcgttg cgtaccaact ttttctcgct
      601 ggcttccgga tcggtgtagc cgtcgaacgg ctgcgctttg gtgaccttgc tcagtagcag
      661 ccccggctgg ccgcggcgca ccttgcgcac ccggccgttc gcatcgcgaa ccgggtcgcc
      721 ggtgtccggg tcgtactcgg cgaacgccac cgtggtgggg cagaggccag cggttttggg
      781 tatgtgaaag atgttgacga accctgtgtt gccttcactg gccccataga actcgaagac
      841 acgcgggatt tcgaagcgag tagtgaactc gtcccagatc tctgggcgta gtccgttgcc
      901 cgcgatgacc cgcaccttgt tggtgcggtc ggtgtccttc tctggctggt tgagcagata
      961 gcggcagacc tcgccgatgt agacgaaggc ggtggcgtcg tagcggacca cctcatccca
     1021 gaaccgcgaa gcagaaaacg accggcccag cgccagtgtg gcaccggcgc tgatcaccgc
     1081 cgacacggcc acggtcagtg cgttgttgtg gtacagcggc aggcagcagt acaacgtgtc
     1141 gctgctcttc aaccgcatgc cgagggcgcc ggacccgccc attgcccgca gccaccggta
     1201 gtgcgtcatc acgctggcct tgggcgctcc ggtggtgccg gaggtgaaga tgtagaacgc
     1261 cctgtccttg gccagtaccg acgatgtcac ctccgggttg gtggcgggcg cgcctgcggc
     1321 caggcgctcc aattcgtcga cggtcaaggt gtcggagata tcgcccgatt cgtcgaccga
     1381 cgcgagcagg tcgtcctcgc cgatcaagac cttggcgcgc agtagcccga cgctgtgcgc
     1441 cagcatgttg ccgtgctgcc ggtggttcag catgccggcg attgcaccga gtttgaccac
     1501 tgcgagcatc agcagcaccg tgcgcggtga attgcgcatc atgatgccca ccacgtcgcc
     1561 gcgagcgacg ccgcgttcgg ccagcaccgc cgcatagcgg ttgacagtgt ggttggcgtc
     1621 gcgataggtc caggcttcgt catcgaagcg gatgaagaca tgatctccgt agcgcgccgc
     1681 tcggtcttga aacaccctgc cgatcgacct cttggctgac cggcgggcaa ggaacgcggt
     1741 gagcacgccg cgcagaacga cgggcgcgtc catcgccagc gcgggtaggc gcacggcgat
     1801 gtcggtcagc ccgacggtgt caccactcat cggtgctagc cgctagggga tcgttgactg
     1861 cggcgccgcc gcgtctgccg gggagaagat aggccggcgg cgtgctcgag ctcgcgcagt
     1921 gccggttcaa tgccgtcggc caactcgtcg atgtcgttcg caatttcggg cgtggtgacg
     1981 aagccgaagt cgaccgatcc gcggtagctg aagcaggtga tgttgagccc gacgtccatc
     2041 accagcggac cgatcggcac cagggtttcc aggaccgcgc cggccatgta gatcgggaaa
     2101 tccggcccgg gcacgttgga gaccaccagg ttgatcggcg cgacgttttc cccgagtcgg
     2161 ctggcggtgt aggcgcgcgc agccagtgcg agcagcccgg gcggcgtgat ctcggtcaat
     2221 cccatgatct ggtgtgcggt gagcgctttg gccatcgctt tggcgccctg cgcgctggcg
     2281 tggatggctc taatccggtc actggggtcc gagttttcgg tgggaagact caccgttgtc
     2341 gaggtgattt ggttgcccac cccagtgtcc tcgccgcggg tggacaccgg tatctgggcg
     2401 agtagcgacc ggtccggaag ctcaccgcgg tccagcaggt agcggcgcag cgccccgcac
     2461 accatcgcca gcacgacatc gttgaccttc acgccgaacg cgtctttgac cgtcttgacc
     2521 cgatccagcg gcacgcggct accgctgacg cgccgatgcg gcgagatcgt ggcattgaag
     2581 cgcgtggtcg gcgcgtcgaa gtagcggggt ggcttgttgg ccagtccgaa caccgccagc
     2641 tgctggcgca cggtctgctc aaccatgcgc aggacccggt agggcgtcat caccgcgagg
     2701 ttgaacattc cgccgagcgc gcggagctcg aaccgcggca tgccgacacc aaccaacgat
     2761 tccttgggct cagccgcggg cgggcgcggt tcgggagtgg cgtcgagcag gatttcactg
     2821 agcccggcgc cggacacgcc atcgatgaga gcgtggtgca tcttggtcag aatggccacg
     2881 cggccgtttt cgacaccctc gatgaaccac agttcccata gcgggcgcgc gcgatcgagc
     2941 ttgtaagaca tcaaccggcc gacaaggtcg tcgagttcct tgcggccgcc cggcggcggc
     3001 accgcgatcc ggcggatgtg aaagtcgatg tcgagctcgg cgtcctccac aagccacggg
     3061 cggtctaacc ccagtggcgc acccgccacc cgataccgca gctgcggcac ttcgggcagc
     3121 cggctggcca gcagctcgcg aacagcatcg aacgagaagt ccgcagcgtt cgacgggtcg
     3181 catatcacca ggccgccgac gtgcatgtgc catcccggtg tctctgcgaa ccaaaacgcc
     3241 gcatcaacac tcgagagccg cttcatctcc accaccgccc cgacgcgcgt gccactgcct
     3301 cggccacggc gagaaactcc cccactgcaa tgatcgtgac ggctatctta gtaataatag
     3361 tgatgaaagg gaacgggttt ctgatccacc gcaccgaacg gcccttcgcg ccccaagttt
     3421 ttatgctcgt ccaagacctc gacgtccgga tcatcaacgc cgccacgggc gaactgatcc
     3481 tcgagctaac catcgatcaa accagggact accacccacg ccacgtcccc agcggccgcc
     3541 ccaaacaaaa agctgtgaac ccaaacgcag gttcacagct atgccgatgt ctagagacgt
     3601 cacagggtgg caggtgcagg attcgaacct gcgtaggcgt tagccgacgg atttacagtc
     3661 cgctcccatt ggccgctcgg gcaacctgcc ttgttgcgac tagcagggta caacgaccca
     3721 gtaccccggt cacaaatcgg cagaagccta ccggccccgc ttgagcgaat ccgcgacaat
     3781 acgcatgtgg ccatcgctcg agcctgcccc actctgctcg tcgtggtggt catcgccggc
     3841 tgcggcggga gcgacccgca gcccacgccg tcgagccact cgtcaagccc gcccaccagc
     3901 gcaactgcgc aaaccccacc aagcagcagc caacccagcg ccgcggtgtc ccccgtgtcc
     3961 caggcggcca gtgcggcggg gttcgtcgac gttcggacca tcgttcccga cgcggtcgtc
     4021 gacctgcggt atgcgacgcc gaacaacttc gtcggggtgc cgctgtatcc aaccgacgcc
     4081 cgctgcctcg tgcacgaatc gatggcgccc ggcttgacga gcgcagcagc agtcctgcgg
     4141 caacaggggg aggcgcttgt cttctgggat tgctatcgac cgcacgacgt ccaggtccgg
     4201 atgttcgagg ccgtcccgaa cccagcctgg gtcgctcggc cgggcagcta cgcacgcagc
     4261 cacgaggccg gccgctcggt cgacgtgaca attgcgcgcg cgcaacagga ttgcccgcca
     4321 ccgcggcaag tggggaaatt ctgtctcgtc gagatgggta ccgacttcga tgacttctcg
     4381 cccagcgcaa ccgcattcgc aaccgacggc gtcagcgccg aagcacaggc gaaccgcgcc
     4441 cgtctacgcg acgccatggg gtccggcggg ttgacggtat acacgggcga gtggtggcat
     4501 ttcgacggtc cgggcgccga cgtcgaacgt ccgattctgg acgtcccggt caattagtca
     4561 gctgcgccgc atggtcgcgt gaatcgtaaa gtcgcgctgc tgcgacgccg aaaagtagac
     4621 agatttgtac agcaccgtgc cgtcgtcctg cggcgtgagt gtgacctccg ctggtacaca
     4681 cgtaccttgg gtgatcttct ccgtgatgaa ccgcacgcca ttccctgcgg gaccgttttc
     4741 ctgccacgta cccgcgcagt tgagttgagg ataggtgaca ttcgcggtca ggggtttatc
     4801 agcggtgatg tcggcgacca cgtcgaagcc ggattggtct ccgctgacgg gccccgacca
     4861 cttgcccacc aacgacgtcg ggaacgcact agtggtcctc gttggtgtgc ccggcagcgt
     4921 cgacggcgtc atcagcggct cgtctgcaat agggacactt gtcgcccggc ttgatgtccg
     4981 cgtggattca gtcgtgtcac cgccgtcgct ggtgcttcgc acgaaggcac cgaccatgac
     5041 ggccaccacc gcgagtgcga gaacagccgc aaggccgagt gcgaccttgg cggctcggcc
     5101 cgttccatgc ctcgccccct tcgcaggagg tgacggcgca ggtggcggcg gcgggggcgg
     5161 aacgacgggt gctgccgacg gcgggtacgc cgacgtcacg gtcgccgggg cggggcgctc
     5221 ggggggtggc cggggaggct gcggcatcgg tgcggtgacg gcagagcggg cggctttggc
     5281 caactccaac gtggtgcgat accgctcgtt gggatccttc gccatgccct tggctatcac
     5341 cgcatcaaag ttcgcgggta cgccgcgccg ggcggtcgac ggtcgcggag gcggcctcaa
     5401 catgtggccg gcaatttggc tcggcacact gtcaccggga tacggctgcg cgcccgtcaa
     5461 gcactcgtac agcacgcaag ccagcgcata gatgtcggct cgcgcgtcta cgtcgccgcc
     5521 gtcgatccgc tcgggagcca tgtactgata ggtcccgatc atcgcggcag tgttcgtcaa
     5581 ccgcgtctcg tcagcaccgc gggcaatccc gaaatcaatc aagtaggcga agtcgaattc
     5641 gttcaccaag atgttggacg gcttcacatc ccggtgcacc aatctcaccc tgtgcgccgc
     5701 gtgcaacgcc tgtgctatct gctcgacgat cgtcaccgcc cgtgcgggcg ccaggggtcc
     5761 cgcacggagg atggtttcca gatcgcggcc ctcgatcagg cgcatgtcca catagaggcg
     5821 accatcgatt tcgccgtagc catggatcgg aatcacatgc ggctcgttga gctgcgccgc
     5881 cgcgcgggcc tcgcgccgga accgctgctg aaatacagcg tcgttggcca attccggtgg
     5941 caagactttg atcgcgacga ttcggtcggt ggctgtgtcg tgcgctcgcc acacttcgcc
     6001 catcccgccg cggcccagca attcgacgaa ctcgtagcgc ccgaacggag tcccgtccac
     6061 catgtccacc cccgtggcca gatagcaaat ccaccataat tcttggcgca gcgcgggtga
     6121 cggcattcgc aaaggccgtg cgcgggcgcg atcgactgcc gtgcacctgc cttggtgcgc
     6181 ccgaagcgct aggacgagaa tgtccccgag ttcacaagta gacgcaccga ggagggccga
     6241 atcccatggc ggattcatcg ttcgatgtcg tgagcaaggt cgaccgccag gaggtcgaca
     6301 acgcgctgaa ccaggccgcc aaggagctgg ccacccgctt cgacttccgc gggactgaca
     6361 ccaccatcgc ctggaagggc gaggaggtca tcgagatcgt cagctccacg gaggagcgcg
     6421 ccaaggcggc agtcgacgtc ttcaaggaga agctcatccg ccgcgacatc tcgatgaagg
     6481 ccttcgacgc gggtgacccg caggccagcg gcaagaccta caaggtcagc ggcaccatca
     6541 agcagggcat cagcagcgag agcgccaaga agatcaccaa gctgatccgc gacgacggcc
     6601 cgaagggcgt caaagctcag atccagggcg acgagatccg cgtcagctcc aagaagcgcg
     6661 acgacctgca gtcggtcatc gccttactga agggtgccga cctcgacgtg gcgctgcagt
     6721 tcgtcaacta ccggtgacgc cggc
//
LOCUS       DASRJA010000274         3590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1718784,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000274 DASRJA010000000
VERSION     DASRJA010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718784"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFC01_12185"
     CDS             complement(<1..571)
                     /locus_tag="VFC01_12185"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZN80430.1"
                     /translation="MASIAITPATSSIQSGQKQQLVATGTDDNGRTIKNLNVKWSSSN
                     PALASVTGNGLVTGIAKGTCVITAQVQGRRATATVTVLDVPVATVAVTLGAAQIAVGQ
                     TTQASVTLKDASGSPITGRTVAWQSSNPAMATVNASGLVTALAKSAVTISAIADAKVG
                     SASLTINAAVPASIAITPSALSVTIGQSGQ"
     regulatory      complement(960..1062)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1439..3430)
                     /locus_tag="VFC01_12190"
     CDS             complement(1439..3430)
                     /locus_tag="VFC01_12190"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HZN80431.1"
                     /translation="MVDPAVASPSLLEGAPTILNVSNGPVTATIQSNWWQSKCVTVRG
                     GTLKDGARVELQSCSGASSQQFVFYSTGEIKIGSKYCLDVFDREPGTSGDPTAIWTCD
                     GTGSQQWKQTSAGELKGANSGLCIGFKGHPYDGTELYLSGCQGSADQKWNAVATTSQA
                     PSSAPASVSTITVALSPASAAVGATSQATATLRDSAGNILTGRTVSWSSSSANVASVT
                     AAGLITALTSGTTNVVGTSEGKTASAVFTVATASSPLPGAACTLVGDHVQRTEPTGVA
                     RPGYLQAMTDPLYKTAITRITGDPGTPIRTAAGQTLGTWGDINTHNYSKDPVWSADQG
                     LIVLKRVKSLGAALFLDGATYQPLFVKSPPGGASETRWHPTIPDEMVIVTSTASVKRW
                     NVRTNVVTTLFTASGYSAADWSWEGDMSTDARYVAIRATRAADGKRVGFRADLVSGAK
                     GPDIDFAANGITDPDVVSVSANGTYVVVTGQIPGAGTCCGGVTDATKVFAAATGAFTG
                     QYFSEYGRPSHYDHAVDASGNEVIVGVSKSAPNEGRVIMRRLVDGVVTVLDQGGYAAH
                     TSARNTGRPGWAYVTHSYNGPNWPPYRDEILAVKLDGSQTIERYGNMHSMNSGGDYDA
                     EPHAVPAPDGRRVIFASAWEAAGVRPVHTYVADARPLCR"
BASE COUNT          561 a         1226 c         1153 g          650 t
ORIGIN      
        1 gttgaccgct ctgcccgatg gtcacggaga gggccgacgg tgtgattgcg atcgaggccg
       61 ggaccgcggc attgatcgtg agcgacgcgc ttcccacttt ggcatcggcg atcgcgctga
      121 tcgtcacagc actcttcgcc agcgccgtca ccagaccgct ggcgtttact gtcgccatgg
      181 ctggattgga gctttgccac gcgaccgtgc gtcccgtgat ggggctgccg ctggcgtcct
      241 tgagagtcac cgacgcctgc gtcgtttgcc caacggcgat ctgcgccgca cctaacgtca
      301 ccgccaccgt ggcgacggga acatcgagga ccgtgaccgt cgctgtcgcc ctgcggccct
      361 gcacttgagc cgtgatcacg cacgtaccct tggcgattcc ggtcaccaga ccgtttccgg
      421 tgacgctcgc cagtgcaggg ttagacgacg accatttgac gttcagattc ttgatggtac
      481 ggccgttgtc atccgtcccg gtcgcgacga gctgctgttt ctgtccggac tggatcgagc
      541 tcgtggctgg cgtgatggcg atcgacgcca cggaggtctt gaggacctga actttcgcga
      601 tacccgtctt ggtcccgagg cgtgcagtga tcgtcgcgct tcccgagtca acgctcgaga
      661 cgacaccgtc cgcggaaacc tgcgctacgc tcgggtctga ggtcgtccac tgagcagtct
      721 ccgttgcggc cgaccccgaa gaatcggcgt tgagcgatgc actgagccga accgagtcac
      781 cgaggatcac gctcgtggcg ctcggcgtga tcgagacggc ggcgagcggc tcttgccggc
      841 ttgcatcgct cgcgctgatg gagtcggcgc cactgcaggc aacgccggcg aatgccatca
      901 cggcggtggc cgccgtgacc atacgagtgg acggtcgcat ggcccctcct ggggttacgt
      961 tcggcggctc agccggcgta acacgcgaag catctgcgcg tgcgtgtgtt agccctgttc
     1021 gctttgcgtc cccgtctttc ggcgggtttg cccttgtcgc gtcacccctc ggggtggtgc
     1081 gatcctgcca ttcgattcgg ttttgtggta tcgggacgat cggttcaaat cactgtcacc
     1141 gagagcgacg gaagccaccg caacatctcg acccgtgata gcccaagtcg ccaactgcgg
     1201 cctgccagtg cgcaactcag tgggagagta aatggggtct ggggcgcgtg ccgtgacgcc
     1261 cgacccatag cggccgtggg gatgagcaca cggctgacca tccgtccagc ctgatggtca
     1321 agcaaggtcc ggtagcaagc aaccgacccg gcagagccta ttcggcttgc cgggtcggtt
     1381 cgaccgtcag tctaggctgc gcgccgaact ggaagcgctg ccaatgggca ggccgccgtt
     1441 agcggcagag gggccgggcg tcagccacgt aggtgtggac cgggcgcacg ccggccgcct
     1501 cccaggcgct ggcgaaaatc acccgccgcc catcgggcgc tggcaccgcg tgcggctccg
     1561 cgtcatagtc gccccccgag ttcatgctgt gcatgttccc gtagcgctcg atcgtctgac
     1621 tcccatcgag cttcaccgcc agaatctcgt ctcggtacgg cggccagttc ggcccgttgt
     1681 agctgtgcgt cacgtacgcc cagcccggcc gccccgtgtt gcgcgccgag gtgtgtgccg
     1741 cgtagccgcc ctggtccagc accgtcacca ctccgtcgac gagccgccgc atgatgaccc
     1801 ggccctcgtt cggcgccgac ttgctcaccc cgacaatcac ctcgttgccg ctggcgtcga
     1861 ccgcgtggtc gtagtgcgac gggcgaccgt attcgctgaa gtactgcccg gtgaacgcgc
     1921 cggtggccgc cgcaaacacc ttcgtcgcgt ccgtgacccc gccgcagcag gtccccgccc
     1981 cgggaatctg cccggtcacc accacgtagg tgccgttggc cgacaccgac accacgtcgg
     2041 ggtcggtgat cccgttggcc gcgaagtcga tgtccgggcc cttcgccccg ctcaccaggt
     2101 ccgcccggaa gccgacccgc ttgccgtcgg ccgcccgcgt cgcccggatc gccacgtacc
     2161 gcgcgtcggt cgacatgtcc ccctcccacg accagtcggc cgccgagtag ccgctcgccg
     2221 tgaacagcgt cgtcaccacg ttggtgcgca cgttccagcg cttcaccgag gcggtggagg
     2281 tgacgatcac catctcgtcc gggatcgtcg ggtgccagcg cgtctcgctc gcgccccccg
     2341 ggggactctt cacgaacagc ggctggtacg tcgcgccgtc gaggaacagc gccgccccca
     2401 ggctcttgac ccgcttgagc acgatcagcc cctggtcggc gctccacacc gggtccttcg
     2461 agtagttgtg tgtgttgatg tcgccccagg tccccaacgt ctggcccgcg gccgtccgga
     2521 tcggcgtgcc ggggtcgccg gtgatccgcg tgatggccgt cttgtacagc gggtcggtca
     2581 tcgcctgcag gtagcccggg cgcgccaccc cggtgggctc ggtacgctgc acgtggtccc
     2641 ccaccagcgt gcacgccgca ccgggcagcg gcgacgacgc ggtcgcaacg gtgaagaccg
     2701 cactggcggt ttttccttca ctcgtcccga cgacgttggt agttccggag gtaagcgcgg
     2761 tgatcaggcc tgcggcggta accgacgcca cattcgccga cgagctgctc caggacacgg
     2821 tccgaccagt caagatattc cccgccgaat ctctcagcgt tgcggttgcc tgggacgttg
     2881 cgccaacagc cgccgacgca gggctcaacg cgacagtaat agttgacacg gacgcgggcg
     2941 cggacgaagg cgcctggctg gtcgttgcga cggcattcca tttctggtca gccgacccct
     3001 gacagcccga caagtatagc tccgtaccgt cgtagggatg ccccttgaat cctatgcaga
     3061 ggccgctatt ggcgcccttg agctcgcctg cgctcgtctg cttccattgc tggctgcccg
     3121 ttccgtcgca ggtccagatg gccgttggat ccccagacgt acccggttcg cggtcaaata
     3181 catcgaggca gtacttcgat ccaatcttta tttcgccggt tgagtagaag acgaactgct
     3241 gagacgaggc acccgagcaa gactgaagtt caactcgtgc tccgtccttt agcgtcccgc
     3301 cgcggaccgt aacgcatttc gactgccacc agttcgattg gatggtcgcc gtaaccggcc
     3361 cgttggagac gttgagaatc gtcggtgcgc cctcaaggag cgacggactg gcaaccgccg
     3421 gatcgaccac gccgggtgcg tcggaggaac acgcggcaat ggcgaaaagg aaagcagcag
     3481 atgctacgga acgactggtc gactgaagtc gaccggcgag gagtcgcgtc aagatggagc
     3541 cctccaggtg caacggcggc tcgactggcc tggcccgcgc acccgcgcgc
//
LOCUS       DASRJA010000275         5164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3429674,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000275 DASRJA010000000
VERSION     DASRJA010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3429674"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VFC01_12195"
     CDS             <1..981
                     /locus_tag="VFC01_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN80432.1"
                     /translation="DACLREGIWLPHACTHGTCGTCKAEILDGEVDFGDASPYALLDS
                     ERAEGCALLCVARPRGDLEVEADVDVEDGVDVHPVRDHCGVVDSVVDIARDVRRVTID
                     LDTPLRFNPGQYVMLNVPGAQQRPYSIASTPSQANRIELHIKRSAGGPATDGWIFKSM
                     TAGERVTLSGPYGRFSLRPAREDPILLLGSGTGLAPLKSMATHLAEIGSGRSVTLYHG
                     VATKDDLYEHDWLEAFTAEHEWFSYRPALSRDSWNGRTGRVPELLAQDYPRAAGHVAY
                     VCGSPAMVTDTVKALMKARLFPRDIYREDFFDSADRAAGVTLVRGPLIRR"
     gene            983..2086
                     /locus_tag="VFC01_12200"
     CDS             983..2086
                     /locus_tag="VFC01_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020170277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZN80433.1"
                     /translation="MKVTELEAIPFAIPYRKPLRFASGEVTVAEHVLVRVHTDVGLVG
                     TAEAPPRPFTYGETQQSIIAVINDVFAAQIIGQSPLQRELIHSRMNRTVGNPTAKAAI
                     DMALWDLIGQSLGVSVTELLGGFTDRLRVSHMVGFAPAAEMVAEAEHMRDVFGITSFK
                     VKVGRRPIDQDLGACRALRLALGPAVELYVDANRGWAVSEAARALRVFAELDMAFAEE
                     PCPADDVLGRRWLVSHSTIPIFADESATRPAEVVRELLSGAATGISIKTARTGFTYSQ
                     RVLNLCEGLGVEVMIGNQIDGQIGSLCALAFGAAFEATSRRAAELSNFLDMADDLLTE
                     PLQIDRGAALVRKGPGLGLHIDEDKLAHYRQDR"
     gene            2121..2957
                     /gene="catA"
                     /locus_tag="VFC01_12205"
     CDS             2121..2957
                     /gene="catA"
                     /locus_tag="VFC01_12205"
                     /EC_number="1.13.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HZN80434.1"
                     /translation="MTDIATAAQSGASATAQFQQRARDGAHASTERVNTLVSEMISAA
                     HDIVRRHHLTYPEYDALKAWLIQVGLDGEWPLFLDVFLEHAVEQEVNAERPGSKGTIE
                     GPYYVPGAPSLPAQTTLPMREDEPGTPLRFAGRVQRVDGGAIDNATIDIWHADDAGFY
                     SQFAPGLPEWNLRGVVHADGQGRFDITTIVPAPYQIPTDGATGTLLAAAGWTAWRPAH
                     LHVKVSAPGYRLITSQLYFDGGEYLHTDIASAVKPELILHPTPAVGQGLEVNYDFVLD
                     AD"
     gene            2976..3269
                     /gene="catC"
                     /locus_tag="VFC01_12210"
     CDS             2976..3269
                     /gene="catC"
                     /locus_tag="VFC01_12210"
                     /EC_number="5.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016159 - muconolactone delta-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase"
                     /protein_id="HZN80435.1"
                     /translation="MIFAVTMDVDLPSDMDAATKADILAREKAYSQELQRSGKWRHIW
                     RIAGQYSNLSIFDVADNDELHAILWQLPLFAFMRIAVTPLATHPSAITEGANG"
     gene            3262..4092
                     /locus_tag="VFC01_12215"
     CDS             3262..4092
                     /locus_tag="VFC01_12215"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZN80436.1"
                     /translation="MADITSLRDAVAQLVADGDTVALEGFTHLIPVAAGHEIIRQRKR
                     HLTLVRMTPDIVYDQMIGAGCAAKLIFSWGGNPGVGSLHRFRDAIQNGWPTPLQIEEH
                     SHAGMANRYVAGASGLPFAVLRGYLGTDLPTVTDTVKTIECPFSGEQLAAVPAINPDV
                     TVIHAQRADRHGNVQLWGLLGVQKEAVLAARRCLVTVEQIVDELTPQPGAIVLPTWVL
                     TAVAEVPGGAHPSYAQDYSERDNAYYAAWDAISRDRSVFREWLDEHVFATSPESGALL
                     "
     gene            4089..4868
                     /locus_tag="VFC01_12220"
     CDS             4089..4868
                     /locus_tag="VFC01_12220"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZN80437.1"
                     /translation="MTITENTTGTEQVDYTTDEMMSVAAARALRDGTRCFVGIGLPST
                     AANLARTTHAPGLVLVYESGTLGSKPARLPASIGDGILAETADAVISVPEVFNYWLQP
                     GRIDVGFLGAAQIDKYGNINTTVIGDYAEPKVRLPGAGGAPEIAAACKEVFVVVRQSN
                     RTFVERVDFITSLGHGHGGEDRHRFGLRGAGPTVVVTDLGVLRPDHTGELVLAATHPG
                     VTVDDVRDATGWQLRTAAKVEVSAPPSAAELRALRALQDKS"
     gene            4865..>5164
                     /locus_tag="VFC01_12225"
     CDS             4865..>5164
                     /locus_tag="VFC01_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN80438.1"
                     /translation="MTPVPLHYIWEGPLLGEPVVLSGSLGSNLRMWDPQVSALTAAGY
                     RVLRYDHRGHGSSPVPAGPYSVADLAGDVVELLDHVNVRRAHFVGLSLGGMVGMWL"
BASE COUNT          953 a         1694 c         1631 g          886 t
ORIGIN      
        1 gacgcctgtc tgcgggaagg gatttggctg ccgcacgcat gcacccacgg cacgtgcggg
       61 acatgcaaag ctgaaatcct ggacggagag gttgacttcg gtgatgcgtc accgtatgcg
      121 cttctcgaca gcgagcgtgc cgaggggtgc gcgctgctgt gtgtcgccag gcctcggggc
      181 gatctggagg tcgaagccga cgtcgacgtc gaggacggag tcgatgttca tccggttcgg
      241 gatcattgcg gcgtcgtcga cagcgtggtc gacatcgcgc gcgacgtgcg ccgggtaacc
      301 atcgacctgg acacgccgct gcggttcaac ccggggcagt acgtgatgct gaacgtgcca
      361 ggcgcccaac agcgtcccta ttccatcgcc agcaccccgt cgcaagccaa tcggatcgag
      421 ttgcacatca aacgctctgc gggaggaccg gccaccgacg gctggatatt caagtcgatg
      481 acggcaggcg agcgagtgac gctgtcggga ccgtacggca ggttctcatt gcgccctgcg
      541 cgagaagatc cgattctatt gctcggcagc ggaacgggtt tggccccgct caaatccatg
      601 gccactcatc tcgcggagat cggatctggg cgttccgtga cgctctacca cggtgttgcg
      661 acgaaagacg atctctacga gcatgattgg ctggaagcgt tcaccgccga gcacgaatgg
      721 ttcagctacc ggccggcgct gtcacgtgac agttggaacg gtcgcacagg ccgcgtccct
      781 gaactgctcg cgcaggacta cccacgcgcc gccgggcatg tcgcctatgt gtgcgggagc
      841 ccggcgatgg tgacagacac ggtgaaggcg ttgatgaagg cgcgtctgtt cccccgtgac
      901 atctatcgcg aggacttctt cgactcggcc gaccgagcgg ccggcgtcac cctggtacga
      961 ggcccgttga tcaggagatg atgtgaaagt cacggaactg gaggcgattc cgttcgccat
     1021 cccgtaccgc aaaccgctgc ggttcgccag cggcgaggtc accgtcgccg agcatgtgct
     1081 cgtgcgcgtt cacaccgacg tcggtcttgt gggcaccgcc gaggcgcccc cgcgtccatt
     1141 cacatacggc gagacccaac aatccatcat cgcagtcatc aacgatgtct tcgccgcgca
     1201 gataatcggt cagtccccgc tgcaacgtga actgatccac tcccggatga atcgcaccgt
     1261 gggcaacccc accgcgaagg cggccatcga catggcgtta tgggatctga tcgggcagtc
     1321 gcttggcgta tcggtcaccg aactgctcgg cgggttcacc gaccggctgc gcgtttccca
     1381 tatggttggg ttcgcccccg cagccgagat ggtcgccgag gccgagcaca tgcgcgatgt
     1441 gttcggcatc acgagcttca aggtcaaggt gggacgtcgg ccgatcgacc aagaccttgg
     1501 cgcgtgccgg gcgctgcggc tcgccctagg ccctgccgtc gaactctacg tcgacgccaa
     1561 ccgcgggtgg gcagtcagcg aagccgcacg cgcgttgcgg gtgttcgccg aactcgatat
     1621 ggcgttcgcc gaggaaccgt gccccgccga cgacgtcctc ggccgtcgct ggctggtctc
     1681 gcatagcacc atcccgatct tcgccgatga aagtgccacc cgcccagccg aagtcgtgcg
     1741 ggaactgctc tctggtgccg ccactgggat cagcatcaag acggcccgca ccggcttcac
     1801 ctactctcag cgcgtcctga acctgtgcga gggacttggt gtcgaagtca tgattggcaa
     1861 ccaaatcgac ggccaaatcg ggtcgctgtg cgccctggcg ttcggcgccg cctttgaagc
     1921 gacatcgcga cgcgcggctg aactgtccaa cttcctcgac atggccgacg acctgctcac
     1981 cgaaccacta cagattgacc gcggtgcagc actggtccgc aaaggtccgg ggctcggact
     2041 gcacatcgat gaggacaaac tcgcccacta tcgccaagac cgctgagtcg ttcggttcaa
     2101 ccagcccaaa ggagtagcac atgaccgata tcgcgaccgc agcccaatct ggggcatcgg
     2161 ccaccgcaca attccagcag agggccaggg acggcgcgca cgccagcacc gagcgcgtca
     2221 acaccctggt gtcagagatg atttccgccg cccacgacat cgtgcggcgg caccacctga
     2281 cctaccccga atatgacgcc ctcaaggcct ggcttattca ggtgggcctc gacggcgaat
     2341 ggccgctgtt cctcgacgtt ttcctcgaac acgccgtgga gcaggaagtc aacgccgaac
     2401 ggccgggcag caagggaaca atcgagggtc cctactacgt ccccggtgcc ccatcgctgc
     2461 ccgcacagac gacgctgccg atgcgggagg acgaacccgg cacaccgctg cgattcgccg
     2521 gccgggtgca gcgggtggat ggtggggcca tcgacaacgc gacgatcgac atctggcacg
     2581 ccgacgatgc cgggttctac tcgcagttcg ccccgggact tcccgagtgg aacctgcgtg
     2641 gtgtggtaca tgccgacggg caaggtcgat tcgacatcac caccattgtg cctgcgccat
     2701 accaaattcc gaccgacggt gctacgggca ccctgctggc cgcggctggc tggactgcgt
     2761 ggcggccggc gcatctgcac gtcaaagtgt cggcgccggg ttatcggctg atcaccagtc
     2821 agctctattt cgatggtggt gaatatcttc ataccgacat cgcgtcagcg gtcaagcccg
     2881 agctcatctt gcacccgaca cccgctgtcg gccagggact cgaagtgaac tacgacttcg
     2941 ttctcgacgc agactgaatc caactgggag acaacgtgat tttcgccgtc acgatggacg
     3001 tcgacctgcc atccgacatg gacgccgcga ccaaagcaga catcttggcc cgggaaaagg
     3061 cctacagcca agagttgcag cggtcgggca aatggcgcca catttggcgg atagccgggc
     3121 agtattcgaa tctcagcatc ttcgacgtcg ccgacaacga cgagctgcac gccatcctgt
     3181 ggcagctgcc gctgtttgca ttcatgcgga tcgcggtgac cccgttggcg actcacccct
     3241 ccgctatcac cgagggcgca aatggctgac atcacatcgc tgcgcgacgc cgttgcgcag
     3301 ctcgtggccg acggcgacac cgtggcgctg gaaggcttca ctcacctgat tccggtcgcc
     3361 gccgggcacg agatcatccg gcagcgaaag cgacatctca cgctggtccg catgacgccc
     3421 gacatcgtct acgaccagat gatcggtgca ggctgcgcag ccaagctcat cttctcgtgg
     3481 ggcggtaatc ctggagtcgg ctccttgcac cgctttcgcg atgccatcca gaacggatgg
     3541 ccgaccccgc tgcagatcga ggagcacagc cacgccggca tggcgaaccg ttatgtcgcc
     3601 ggcgcctccg gtctgccgtt cgcggtgttg cgcggatacc tgggtaccga tctgccgacc
     3661 gtcaccgaca cggtgaagac catcgaatgt ccgttcagcg gagagcaact cgcagcggta
     3721 cccgcgatca accccgacgt gaccgtcatc catgcacagc gggccgatcg gcacggcaat
     3781 gtgcaactgt gggggttact cggtgtgcaa aaggaagccg tgttggccgc gcgccgctgc
     3841 ctggtcaccg tcgaacagat tgtcgacgaa ctcacgccac agcccggcgc gatcgtcctg
     3901 ccgacctggg tgctgaccgc agtcgccgaa gtaccaggcg gcgcccaccc gtcatatgcg
     3961 caggactatt ccgagcggga caacgcatac tacgcggcct gggacgcgat cagccgcgac
     4021 cgctcagtgt ttcgcgagtg gttggacgag cacgtgttcg cgacgtcacc cgagtctgga
     4081 gcgctcctat gacgatcacc gaaaacacca ctggcacaga gcaagtggat tacaccacgg
     4141 atgagatgat gtcagtggcg gccgcgcggg cgctgcggga cggcacccgc tgcttcgtcg
     4201 gaatcggact tccgtcaacc gcggccaatc tggctcgaac gacgcatgcg ccagggctgg
     4261 tgctggtgta cgaatcggga acgctgggct ccaagccggc ccggttgccg gcatccatcg
     4321 gcgacggcat cctcgccgaa accgccgacg cggtgatcag tgtccccgag gtgttcaact
     4381 attggttgca gcccggccgc atcgacgtcg gcttcctcgg cgcggcgcag atcgacaagt
     4441 acggcaacat caacacgacg gtgatcggcg actacgccga gcctaaagta cgactgcccg
     4501 gggccggagg cgcccccgaa atcgccgcgg cgtgtaaaga ggtgttcgtc gtcgtgcggc
     4561 aatcgaatcg cacgttcgtc gagagggtcg atttcatcac ttcgcttgga cacgggcacg
     4621 ggggagaaga ccgccaccgg ttcgggctgc gcggcgcagg accgaccgtg gtggtcacgg
     4681 acctgggcgt gctccgcccg gaccacaccg gtgaacttgt cctggcagcg acgcaccctg
     4741 gtgtcaccgt cgacgacgtt cgcgacgcga caggctggca actgcggacc gccgccaagg
     4801 ttgaggtcag cgcaccaccg tccgcggcgg aactgcgggc gctgcgtgca ctccaggaca
     4861 aatcatgacc ccggtgccgc tccactacat ctgggagggg ccactcctcg gcgagccggt
     4921 agtgctcagc gggtcgctcg gcagcaacct gcgcatgtgg gatccacagg tcagcgccct
     4981 gactgcggcc ggttaccgcg tgctgcgtta cgaccaccgc ggccacggtt cctcaccggt
     5041 gccggcggga ccctacagcg tcgccgacct agccggcgat gtcgtcgagc tccttgatca
     5101 cgtgaatgta cggcgcgccc acttcgtcgg tttgtcactt ggcggcatgg tcgggatgtg
     5161 gctg
//
LOCUS       DASRJA010000276         4238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3148641,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000276 DASRJA010000000
VERSION     DASRJA010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3148641"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VFC01_12230"
     CDS             complement(<1..818)
                     /locus_tag="VFC01_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80439.1"
                     /translation="MRSFVAVLLVGAPTAAQASWSMLYPSAAPLPVNSPSLACFEPTG
                     EVVLLFGIASVPATQGWRLVGATWSPFPMPAQTGYGRGMVYDSARQRLIVFGGNGPAN
                     DDTWEWNGVSWLHPIPAVRPAARTNFAMAFDRTRGIAVVFGGNDGSGQHLQDLWEWNG
                     VTWTQRAAVTPLGLRARVLGAFDPVHRNVLLYGGSRPFAGSTLVFNDTWAWDGTSFTQ
                     YAPPVPPPYLQEAAIVTDLHRQRVILYGGLPADGVTREWTGAEWVDRTVPSPGPR"
     gene            881..1063
                     /locus_tag="VFC01_12235"
     CDS             881..1063
                     /locus_tag="VFC01_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80440.1"
                     /translation="MMRAALDVSAVRRRSQAPAAHRNRPAGTDAATSGTAEVVAGSSA
                     GDRQGYLGHAAHAHLT"
     gene            1166..2641
                     /locus_tag="VFC01_12240"
     CDS             1166..2641
                     /locus_tag="VFC01_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HZN80441.1"
                     /translation="MPRATSCPTPSLGEVSQSGCQRGEQWSPASFGTGLARRRPQPAL
                     HCTPSAASIRRFGWQQPIVARRSGEVIAGNTRLKAAQELGMTEVPVWWLEGTDLDAVA
                     FGIADNRTHTFSEFDEPALVKLLEQLKAEDALDGVGYSTEDIDALVQKLREQEEVDKQ
                     LDDDGPEDPPAVAIAKTGDLWCLGDHRLLCGDSTSLKDVHRVMGSDKAALVATDPPYL
                     VDYTGERPNDSGKDWTATYREIDIQDADGFFRAVFVNVLEVLGPKGAIYCWHAHKRSG
                     DIQRTWRELGILDHQSVIWIKPTPVFGRVYWHFRHEHCLMGWREGSKPEHDGVHEHDS
                     VWEVDWDGKSRIVGNEHPCTKPVELFVRPIKKHTKVGDVVFEPFSGSGSQLIAAERTG
                     RKCHAIEISPPYIDVAIRRWQKATGKDATLDGDGRTFNEIAAEPRFCRRRSAPEPGAA
                     GTPSIRAGGQVKARCVRAASNWLELPENGRTRCLHTLCDEG"
     gene            2835..>4238
                     /locus_tag="VFC01_12245"
     CDS             2835..>4238
                     /locus_tag="VFC01_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80442.1"
                     /translation="MGALGVAPAQAPPPGAWVESFSHKSIDWPPGNQEATDHLIPFGG
                     HLNANISYYIDAVSNVYRWPHAKDSLTGGRAFDPNRPWFMFNAGHAALIPKGPHQGKV
                     LVWVESPFLIKPDPTFSPVVLGQHDYWGCQAYSIVDPSASALPRIFRNFVLPIAVVNI
                     QPGAGASSLTDLFCAGQAWSPYGDLIVVGGQMYIPPLLGSKLTYAWNPDEPVGQWPVP
                     SPPPTSLYPGEFGFWQPGPMLQWDRWYPTATLTARLTRLATAAAPLGLEVMLVLGGSA
                     AFNFQYWTNPQWNSYEALQILGRATPTSVALATDVAFGMQTWTGPGTTGPPGPSPWMD
                     WQQDWFENYPRCHLLSSGHVFFSGYAPRWSAVDHDLAPGSWISDQQPVPPPPPPPVWS
                     SNWNHMRYDGTSLLFPNIFGLVDVVARIGGAAEIPAPIVFGTTESAEAWLPSTGEWAP
                     LSPMPPNPSPSPNGRSQM"
BASE COUNT          749 a         1421 c         1354 g          714 t
ORIGIN      
        1 cgtggtcccg gcgaaggcac cgtccggtcc acccactcgg cgcctgtcca ctcgcgcgtg
       61 acgccgtcgg caggtaagcc cccgtacagg atcactcgct gccgatgcag gtcggtgacg
      121 atcgcagcct cctggagata cggcggaggt acgggcggcg cgtactgagt gaacgacgtg
      181 ccatcccacg cccacgtgtc gttgaacacc agcgtactgc cggcgaacgg ccggctgcca
      241 ccgtacagga gcacgttccg atggacgggg tcgaacgcac ctaacactcg ggcgcgcagg
      301 cccagcggcg tgacagcagc gcgctgcgtc cacgtcacgc cgttccattc ccacagatcc
      361 tgcaggtgct gcccgctgcc gtcattgcca ccgaagacga cggcgatgcc acgggttcga
      421 tcgaaggcca ttgcgaagtt ggtgcgcgcc gccggcctca ccgccggaat cggatgcagc
      481 cacgaaacac cgttccactc ccaggtgtcg tcattggccg ggccgttgcc gccgaacacg
      541 atgagccgct gccgcgccga gtcgtagacc atccctcggc catagcccgt ctgcgccggc
      601 atcgggaacg gactccaggt ggcgccgacc agccgccacc cctgcgttgc cggcacgctt
      661 gcgatgccga acagcagaac gacctcgccc gttggctcga agcacgccaa cgacggagag
      721 ttgaccggca acggggcggc cgacggatac agcatggacc agctggcctg ggctgcggtg
      781 ggcgcgccga ccaagaggac tgcgacgaac gaacgcatgt gcatggactg tctccggggg
      841 cagcggagtg tatcaggggt gtgagggctg cgttgccgtg atgatgcgcg ccgcactcga
      901 cgtcagcgcc gtccgccgac gttcccaggc tcctgcggcg caccggaatc ggcccgcggg
      961 gactgacgcg gccaccagcg ggaccgccga agtagttgcc ggcagctcgg ccggcgatcg
     1021 gcagggctac cttggccacg ccgcgcacgc gcacctgacc tgagtctcca acttccccga
     1081 ggctacaccg attccggccc tgaatcgcgg tcctggcgcg ggcgttcaca caacacaacg
     1141 accgagcgat ttgtgacttg cgcgcgtgcc tcgggcaacc agttgcccaa cgccgtcgtt
     1201 gggggaggtc agccaatccg gctgccaacg cggcgagcag tggtcgccgg catcgttcgg
     1261 caccggtctc gctcgtcgcc gcccgcagcc cgcgctacac tgcaccccgt ccgcggcctc
     1321 gattcgccgc ttcggctggc agcagcccat cgtcgctcgc cggtccggtg aggtgatcgc
     1381 gggtaacacc cggctcaagg ccgcgcagga gctcggcatg acggaggtgc cggtgtggtg
     1441 gctcgagggc accgacctcg acgccgtggc gttcggaatc gcggacaatc gcacgcacac
     1501 cttctccgag ttcgacgagc cagctctcgt gaagctgctt gagcagctca aggccgagga
     1561 cgctctcgac ggcgtcggct attcgaccga ggacatcgac gcgctggtgc agaagctccg
     1621 cgagcaggaa gaagtcgaca agcagctcga cgacgacggc cccgaggatc cgccggcagt
     1681 cgcgatcgcc aagaccggcg acctctggtg cctcggcgac caccgcctgc tctgcggcga
     1741 ctcgacctcg ctcaaggacg tgcaccgcgt gatgggcagc gacaaggccg cgctcgtcgc
     1801 gaccgacccg ccgtacctcg tcgactacac gggcgagcgc ccgaacgact cgggcaagga
     1861 ctggaccgcg acgtaccgcg agatcgacat ccaggacgcc gacggattct tccgtgcggt
     1921 gttcgtgaac gtgctcgagg tgcttgggcc gaagggcgcg atctattgtt ggcacgcaca
     1981 caaacgcagc ggtgacatcc agcggacctg gcgcgagctc ggcatcctcg atcaccagtc
     2041 ggtgatttgg atcaagccga cacccgtgtt cggccgcgtc tactggcact tcaggcacga
     2101 gcactgcctg atgggctggc gcgaaggctc caagcccgag cacgacggtg tacacgagca
     2161 cgattcggtc tgggaggtcg actgggatgg gaagtcgcgg atcgtcggca acgagcatcc
     2221 ttgcacgaaa cccgtcgagc tgttcgtgcg gcccatcaag aaacacacga aagtcggcga
     2281 cgtcgtgttc gagcccttca gcggatcggg cagccagctc atcgcggccg aacgcacggg
     2341 caggaagtgc cacgcgatcg agatctcgcc tccctacatc gacgtcgcga ttcgtcgttg
     2401 gcagaaggcc accggcaagg acgcgacgct ggatggtgac ggccgcacgt tcaacgagat
     2461 cgcggccgag ccgcggttct gcaggcggcg gtcggctcct gagccggggg cggctggtac
     2521 gccgtcaatt cgggcaggcg gacaagtcaa agcaaggtgc gttcgggcag cgagcaactg
     2581 gctggagctg cccgagaacg gtcgcacacg atgtctccac actctgtgtg atgaaggctg
     2641 attcacccgc atctcatgcg gcgctcattc ccgaagggca ccattcacgt tgcttcctcg
     2701 tcggcagggc caacgacggt gctctgcaca gggggcccct aacgtgcgag gtaaccatga
     2761 ccacttcggt tcgagcaaaa gaacggagag aaggcgccgc ggcgatgcgg cttgtgtgta
     2821 gcacgctgat cggtgtgggc gcactcggtg tcgcgcccgc ccaggctccg ccgcctgggg
     2881 cttgggtcga gagcttcagc cacaagagca tcgactggcc gccaggaaac caagaagcga
     2941 cagatcacct gattcccttt ggtggccatc tcaacgccaa catcagttac tacattgatg
     3001 ctgtcagcaa cgtctatcgc tggccccacg caaaggactc cctgacgggg gggcgggcat
     3061 tcgatcccaa ccgtccctgg ttcatgttca acgccggcca tgcggcgttg attcccaagg
     3121 ggccccacca gggcaaggtg ctcgtctggg tagagtcgcc ttttctaatc aaacctgatc
     3181 cgaccttcag tccggtggtg cttggccagc acgactattg ggggtgccaa gcgtactcga
     3241 tcgtcgatcc ctctgcgtct gcgctaccga ggatcttccg caacttcgtc ttgccgattg
     3301 cggttgtgaa cattcaaccc ggggcggggg cgtcctcttt gacggacttg ttttgtgcag
     3361 gacaggcttg gtcaccctac ggtgatctca tcgtcgtcgg tggacagatg tacatccccc
     3421 cgctcctcgg gtcaaaactg acgtatgcgt ggaatcccga tgagcccgtc ggtcagtggc
     3481 cagtcccctc tccacctcca acgtcgctct accccggtga gttcggcttc tggcagcccg
     3541 gtccgatgct gcaatgggat cgctggtacc ccaccgcgac gttgacggca cgtctcaccc
     3601 ggttggccac agctgccgct ccgttggggc tcgaggtaat gctcgtactt ggtggcagcg
     3661 ccgccttcaa ttttcagtat tggaccaacc cgcagtggaa ctcgtacgag gcgctgcaga
     3721 tcctcggtcg tgctacgccg acgagcgtgg cattggcgac tgacgtcgcg ttcggcatgc
     3781 agacgtggac tggtcctggc acgacgggac ctccgggtcc gtccccctgg atggactggc
     3841 agcaggattg gttcgagaac tacccgagat gccacctgct cagttcgggc cacgttttct
     3901 tctccggcta tgcacctcga tggagcgccg tcgaccatga tctggcgcca ggttcctgga
     3961 tctcagacca acagccagta ccgccgccac cgccgccacc ggtgtggagt tcgaactgga
     4021 accacatgcg gtacgacggc acgagcctgc tcttccccaa catattcggg ctcgtggacg
     4081 tcgtcgctcg gattggcggt gcggccgaga tcccggcccc gatcgtcttc ggtaccacgg
     4141 aatccgccga ggcatggtta cccagcacag gagaatgggc tcccctgagc cccatgcctc
     4201 ccaaccccag cccttcgccg aacgggcgct cacagatg
//
LOCUS       DASRJA010000277         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1256296,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000277 DASRJA010000000
VERSION     DASRJA010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256296"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VFC01_12250"
     CDS             complement(<1..1217)
                     /locus_tag="VFC01_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80443.1"
                     /translation="MRRNLTAALLSGALLLGAMGTSHAVYDETTIYAIQQDMIAIGDS
                     VLVDSVVVIGVDIRPGTYGVYIQEPNSGPYSGILAYRNTIFPAYENSGLAVTVGDLIT
                     VVGRYADFAGLAEIDVPLLTRLGTVTPLAPTVLTVDDLKTVNPNSEQWEGVLVRVNDV
                     KVTSIEAPFNNWRFHMNSGPPAGQVDSLVAYEKMISGQVVPQVGDILASVTGVLDWAF
                     NDRRIAPRGDNDIVFTSPAPAPVPNLAYSTAENKIKVRFNVNLQTASAQTTTNYSLST
                     LQPITSAVYDNATKTVTLTTGSNLVPSTTPHTLSMSGIRNSQNVIMSGTQAISFIGGI
                     APITFVQTPKSASNDTSQVANQQVSMRGVVTETTGVDYPASIGGFYLQQRGAVEYAGI
                     FIFGAAITPVRGD"
     gene            complement(1276..2742)
                     /locus_tag="VFC01_12255"
     CDS             complement(1276..2742)
                     /locus_tag="VFC01_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80444.1"
                     /translation="MARITVCVALLALACDDTIEPRPPKQGNVAPETYLVTESDSLRP
                     QFYGIRLQWLGTDADGRVVRYRYRWDEVCTQEPCPVPPDWIETTALFGDFTLPVPGDS
                     ALYQFEIAAVDNNGQPDESPASQEFDFRNAAPTVEFVPGTLPTRTLPAVTFILSGTDP
                     DTTETPEDADTTAHLTVYRSWLNGNEAQVIETPFAQGSITLRPEDFAGGPAAGDSVRT
                     VFVQVLDDGLAVSPAIQHTWLVDEPPVNGILLVDDCRMGGMLEDFSDQSFRRVLETWA
                     PGRYVVMDIDLISYPNAPDFEAQLSLFDRIVWYTDADTTSSGALELARGGLEALLDRN
                     GRLFLSSGLVFGTRSAFGDRETRFRESFGIGSVFTAPLGGTNFSISLVDTVHAAVHPG
                     LERFTFLSQGLRGIVECFDSVSDVDTRSLYFYPESTLVRDTFVNPVQYDVGVSRLQAG
                     GPSTVYVSFPIGIPINSNMGENEIEIREMLRLAGILEP"
     gene            complement(2822..>3512)
                     /locus_tag="VFC01_12260"
     CDS             complement(2822..>3512)
                     /locus_tag="VFC01_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80445.1"
                     /translation="VYLSNSSVADSFRVVRDVDVRDGNGFDTGLEGVRRSTPYYDGPD
                     TLLYELRLDGIPDGLKRYAAVTSYDAQLVVPYSLESGVLQNSTYFIAGPSAAQAVHQR
                     VSVFPNPYRGESAFDGRDAAGALNPRRRVLWFTNLPPRAHLEIFTLAGDRVRTYEYDA
                     ATYRGTEAAGISPDNADLAQGRYLTSGGSMLAFDLLSENGQEIATGLYLFAVENKDTG
                     DKQQGKFLVLK"
BASE COUNT          617 a         1134 c         1130 g          631 t
ORIGIN      
        1 ctgtcaccac gcactggcgt gatggcagcg ccgaagatga agatgccggc gtattcgacc
       61 gcaccgcgtt gctgcaggta gaagccgccg atgctcgccg ggtagtcgac tccagtggtt
      121 tcggtcacga cgccgcgcat cgagacctgc tggttggcca cttggctggt gtcattgctc
      181 gccgacttcg gcgtctgcac gaacgtgatc ggcgcgatgc cgccgatgaa actgatcgcc
      241 tgcgtaccgc tcatgatcac gttctgggaa ttgcgaatgc cgctcatcga caaggtgtgc
      301 ggcgtcgtcg atggaaccag gttgctaccc gtcgtcaagg tcaccgtctt ggtcgcattg
      361 tcgtacaccg cagacgtgat cggctgcagc gtgctgaggc tgtagttcgt cgtcgtctgc
      421 gccgaggcgg tctgcaggtt gacgttgaac cgcaccttga tcttgttctc ggcggtcgag
      481 taagccaggt tcggcaccgg tgccggcgcc ggcgacgtga acacgatgtc gttgtcaccg
      541 cgcggggcga tgcggcgatc gttgaaggcc cagtccaaca cgccggtcac cgaagccagg
      601 atgtcgccca cctgcggcac gacctggccc gaaatcatct tctcgtaggc caccaaactg
      661 tcgacttgcc ccgcgggcgg cccggagttc atgtggaagc gccaattgtt gaacggggct
      721 tcgatggacg tcaccttgac gtcgttgacc cgcaccagca cgccttccca ctgttcggaa
      781 ttcgggttga cggtcttgag atcgtccacc gtcagcacgg tcggcgcgag cggcgtcacg
      841 gtgccgagtc gggtcaggag cggaacgtcg atctccgcca agccggcgaa gtccgcgtag
      901 cgtccgacca ccgtgatcag gtcgccgact gtgaccgcca ggccgctgtt ttcgtaggcc
      961 gggaagatcg tgttgcggta ggcaagaatc ccgctgtagg gaccgctgtt cggttcctgg
     1021 atgtacaccc cataggttcc ggggcggatg tccacgccga tcacgacgac gctgtccacc
     1081 agaaccgagt cgccgatggc gatcatgtcc tgctggatgg catagatcgt cgtctcgtcg
     1141 tacaccgcgt gtgatgttcc catcgcgccg agaagtagcg cgccggacaa gagggctgca
     1201 gtcaggtttc gtcgcatctt tcgtcgtcct cccaacgagt gcgggtcacg gcgcgcgcgc
     1261 gccgcgacgg ctctcctacg gttccaaaat gcccgcgagg cggagcatct cgcgaatctc
     1321 gatttcgttt tcacccatgt tgctgttgat cggaatgccg atcgggaagc tcacgtacac
     1381 ggtggacggc ccaccggcct gaagtcgtga gacaccgacg tcgtactgca ccggattgac
     1441 gaacgtgtcg cgcaccaacg tggactccgg atagaaatag agactgcgcg tatcgacatc
     1501 cgaaaccgag tcgaagcact cgacgatgcc gcgcaacccc tggctcagga acgtgaaccg
     1561 ctcgaggcct ggatgcaccg cggcgtgcac ggtgtcgacc agggagatcg agaagttcgt
     1621 gccgcccaag ggtgccgtga acacggagcc gatcccgaac gactcgcgga agcgtgtctc
     1681 gcggtccccg aaggcgctgc gcgtcccgaa cacgagccca ctcgacagga acaatcgtcc
     1741 gttccgatcg agcaaggctt cgagcccgcc acgcgccaac tccaatgcac ctgacgatgt
     1801 cgtgtcggcg tcagtgtacc atacgatgcg atcgaagagg gagagctggg cctcgaaatc
     1861 gggggcgttc gggtacgaga tgagatcgat gtccatgacg acgtagcgtc ctggggccca
     1921 tgtctcgagg acacgccgga acgattgatc cgagaagtct tcgagcatcc ctcccatgcg
     1981 gcaatcgtcc accagcagga tgccgttcac cggcggttca tccaccagcc aggtgtgttg
     2041 aatggcgggg ctcacggcca acccgtcgtc gagcacctgc acgaacacgg tgcgcacgct
     2101 gtcgccggcg gcggggccgc cggcaaagtc ctccggtcgc agcgtgatgc tgccctgcgc
     2161 gaatggcgtc tcgatcacct gcgcctcgtt gccgttcagc caggagcggt agacggtcaa
     2221 gtgggcggtg gtgtcggcat cctccggggt ttcggtggtg tccgggtcgg tccccgatag
     2281 aatgaaggtc accgccggca gggtccgggt cgggagcgtc cccggaacga actccaccgt
     2341 cggcgcggcg ttgcggaaat cgaactcctg cgacgccggc gattcgtccg gctggccgtt
     2401 gttgtccacc gcggcgatct cgaactggta cagcgccgag tcccccggca caggcagcgt
     2461 gaaatcaccg aagagagccg tggtttcgat ccagtccggc gggaccggac agggctcctg
     2521 ggtgcagacc tcgtcccagc gatagcgata gcgcaccacg cgcccgtcgg cgtcggttcc
     2581 gagccactgc aaacgaatcc cgtagaactg cggccgcagg ctgtcggatt cggtcaccaa
     2641 gtaggtttcg ggcgctacat tgccctgctt cggcggcctc ggctcgatcg tgtcgtcgca
     2701 cgccagcgcc aagagcgcga cacagaccgt gatccgcgcc acgcgagccg aatccggccg
     2761 ccggcccggc tcgagtctgt cgcttcgccg cctcaggtgc atcgcgctcc cactccgacc
     2821 ttcacttgag caccaggaac ttgccctgct gcttgtcgcc ggtgtccttg ttctccaccg
     2881 cgaagaggta caacccggtg gcgatctcct gtccgttctc gctcaagagg tcgaacgcca
     2941 acatcgatcc gccggacgtc aagtaacgcc cttgcgccag atcggcgttg tcggggctga
     3001 tccccgccgc ttcggtgccg cggtaggtcg ccgcgtcata ctcataggtg cggacgcggt
     3061 cccctgcgag agtgaaaatc tccagatgcg cccgcggcgg caggttggtg aaccacagca
     3121 cccgacgccg tggattgaga gcgcccgccg cgtcgcgccc gtcgaacgcc gattcgccgc
     3181 gatacggatt cgggaacacg ctgacgcgct ggtgcaccgc ctgggccgca ctgggcccgg
     3241 cgatgaagta agtgctgttc tgcagcacgc cgctctcgag cgagtagggc accaccagct
     3301 gcgcgtcgta ggacgtcacc gcggcatagc gcttgaggcc gtcggggatg ccgtccaaac
     3361 gcagctcgta cagcagtgta tcggggccgt cgtagtaggg cgtactgcgc cgcacaccct
     3421 cgaggccggt atcgaagccg ttgccgtcgc gcacatcgac atcgcgcacg actcggaagc
     3481 tgtccgcgac cgaactattg ctgagataga cg
//
LOCUS       DASRJA010000278         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_938074,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000278 DASRJA010000000
VERSION     DASRJA010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938074"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFC01_12265"
     CDS             <1..189
                     /locus_tag="VFC01_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HZN80446.1"
                     /translation="AMGYKELPGSLGVALTEMEGSELVAEALGEHVFDFFLRNKRTEW
                     ENYRSHVTPFELKAYLSL"
     gene            215..3157
                     /locus_tag="VFC01_12270"
     CDS             215..3157
                     /locus_tag="VFC01_12270"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HZN80447.1"
                     /translation="MAKPATQRPKLPSVGRLGLVGPQAPADLDRLGWNTDAHVELLWS
                     LSRAPDADIALRAMVRLADAVGRGWDELNQSLVKDRGLRGRLFGVLGSSLALGDHLVA
                     HPDSWHLLAGDVTLPSADELRAMFAGEAEKASDTTGALQPLRTLHRNRLLVLAGLDLA
                     PTVENEPVLPFTTVGEHLSDIADAALGAALIVAGGDEPPRLAVIAMGKCGARELNYVS
                     DVDVIFVGETADAVETRVAGEMMRFASDAFFEVDAALRPEGKHGQLVRTLDSHIAYYQ
                     RWAKTWEFQALLKARPAAGDEELGRRYVEALMPMVWTASQREDFVPEVQAMRRRVEEL
                     VPAGVRAREIKLGTGGLRDVEFAVQLLQLVHGRNDESLHVASTVDALAALGSGGYIGR
                     DDAANMTASYEFLRLLEHRLQLQRLKRTHMLPEPDDDEALRWLARAAHVRPDGQHDAL
                     GVLREELKRQNMRVSRLHAKLFYQPLLESVGSVGISEGMTPEAAERQLAALGYEGPQS
                     ALTHLAALTGHSGRRGRVQQVLLPTLLDWLSDTPDPDAGLLAYRRISEELADHRWYLS
                     TLRDEGAVAKRLMRVLGTSAYVPELLMRAPEVIQLYADGPGGPKLLEVEPDAVARALV
                     ASAGRHPEPVRAIAAARTLRRRELARIASADLLGMLKVTDVCNALTSVWVAVLQAALD
                     AIIRANTPDSGAPARIAVIGMGRLGGGELGYGSDADVMFVCDPEDGVDESAAVRWAVT
                     IAEQVRTLLGTPSADPPLEVDANLRPEGRNGSLVRTLASYETYYDQWAQPWEVQALLR
                     AHRVAGDPELGERFLLMVDKTRYPSGGVSAEAVQEIRRIKARVDAERLPRGADPNTHT
                     KLGRGGLADIEWTVQLLQLRHAHKVPALHSTSTLDTLDAIGRAELIAEGDVELLRQAW
                     LTATRARNALVLVRGKPTDQLPGPGRQLNAVALAAGWDSDDGGEFLDNYLRVTRRAKA
                     VVRKVFGE"
     gene            3154..>3268
                     /locus_tag="VFC01_12275"
     CDS             3154..>3268
                     /locus_tag="VFC01_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN80448.1"
                     /translation="MSMDFGFDVISAEEYDRQRVLYEPLTAAIRRLIDAGIH"
BASE COUNT          524 a         1067 c         1173 g          504 t
ORIGIN      
        1 gcgatgggct acaaggagct acccggcagc ctcggtgtcg cgctgaccga gatggagggc
       61 tccgagctgg tcgcggaagc actgggggag cacgtattcg acttcttcct acgcaacaag
      121 cgcaccgaat gggagaacta ccgcagccac gtcactccgt tcgagctgaa ggcctacttg
      181 tctctctagc ccgggcgcgc gataacgtca cctaatggcc aaacctgcga cgcagcgtcc
      241 gaagctgccc agtgttggca ggctcgggct ggtcggcccc caggcccccg cggacctgga
      301 tcggctgggc tggaacacag acgcgcacgt cgagctgttg tggtcactgt cgcgcgcgcc
      361 cgacgccgac attgcgctgc gggcgatggt ccggctcgct gacgccgtcg gacggggctg
      421 ggacgaactc aatcagtccc tcgtcaagga ccgagggctg cgcggccggc tgttcggtgt
      481 gctcggctcg tcgttggcgc tcggtgatca tctggtcgcc catcccgact cgtggcacct
      541 attggcgggc gacgtcacac ttccgtccgc ggacgagttg cgggcgatgt tcgccgggga
      601 ggccgaaaag gcaagcgaca caaccggtgc gctgcagccg ctgcgaacgc ttcaccgcaa
      661 ccggctgctg gtgctggcag ggcttgatct cgcgccgacg gtggagaacg agccggtgtt
      721 gcccttcacc accgtcggcg agcacctgtc cgatatcgcc gacgccgcgc tgggcgctgc
      781 attgatagtg gccggcggcg atgagccgcc gcggctcgcg gtgatcgcga tgggcaaatg
      841 cggcgcgagg gagctgaact acgtcagcga cgtcgacgtc atcttcgtag gcgagaccgc
      901 agacgccgtc gagacccggg tggcaggcga gatgatgcgg ttcgccagcg acgcgttctt
      961 cgaagtggac gccgcactgc ggcctgaggg caagcacggc cagctggtgc gcaccctgga
     1021 ctcccacatc gcctactacc agcgctgggc caagacctgg gaattccagg cgctgctcaa
     1081 ggcgcggccg gcggccggtg acgaagagct aggccgacgg tacgtcgagg cgctcatgcc
     1141 catggtgtgg accgccagcc agcgtgagga cttcgtgcct gaggtgcagg caatgcgcag
     1201 gcgcgtcgag gagctcgtgc cagccggggt gcgggcccgc gaaatcaagc tgggcacggg
     1261 cgggctgcgc gacgtcgagt tcgccgtgca gctgctgcag ttggtgcacg gccgcaacga
     1321 cgaatcgctt cacgtcgcct cgaccgtgga cgcgctggcc gcgctcggtt ccggcggcta
     1381 catcggccgc gacgacgccg ccaacatgac cgcctcctac gaattcctgc ggctgctcga
     1441 acaccgattg cagcttcagc ggctcaagcg cacacacatg ctgcccgagc ccgacgacga
     1501 cgaggcgctg cgctggctgg cccgcgcggc acacgtgcgc cctgacggtc agcacgacgc
     1561 actcggcgtg ctgcgcgagg agctcaagcg ccagaacatg cgggtgtcgc ggctgcacgc
     1621 gaagcttttc tatcaacctc tactggaatc cgttggctca gttggtattt cggaaggcat
     1681 gacaccggag gccgccgaac gccagctcgc cgcgctgggc tacgagggcc cgcagagcgc
     1741 gctgacacat ttggcggcgc tgaccggtca cagtggcagg cgtggccggg tgcagcaggt
     1801 gttgctgccg acgctgctgg actggctgtc cgacaccccc gatccggacg ccgggctgct
     1861 ggcgtaccga cgcatcagcg aggagctggc cgaccatcgg tggtatctgt ccacgctgcg
     1921 cgacgagggc gcggtggcca agcggctgat gcgggtgctc ggcacctccg cttacgtgcc
     1981 tgagctgctg atgcgggcac cggaggtgat ccagctctac gcggacggtc cgggcggacc
     2041 caagttgctc gaggtcgagc ccgatgcggt ggcacgggcg ctggtcgcct cggcgggcag
     2101 gcatccggaa ccggtgcggg ccattgccgc cgcccgcacg ctgcggcgcc gcgagctcgc
     2161 ccgcatcgcg tcggcagacc tgctcggcat gctcaaggtg accgacgtgt gcaacgcgct
     2221 gacgtcggtg tgggtggccg tgctgcaggc tgccctggac gcaatcatcc gggccaacac
     2281 cccggacagc ggcgcgccgg cccgcatcgc ggtgattggc atgggccgac tgggcggcgg
     2341 cgagctgggc tacgggtccg acgccgacgt gatgttcgtc tgtgacccgg aggacggtgt
     2401 cgacgagtcg gcggcggtgc ggtgggcggt gaccatcgcc gagcaggtcc gcacgctgct
     2461 cggcaccccc agcgccgatc cgccgctgga ggtggacgcg aacctgcggc cggagggccg
     2521 caacggttcg ctggtgcgca cgctcgcctc ctatgagacc tactacgacc aatgggcgca
     2581 gccgtgggag gttcaggcgc tgttgcgggc gcatcgagtg gcgggtgatc ccgaactcgg
     2641 tgagcggttc ctgctaatgg tcgacaagac ccgctacccg tcgggtgggg tgtccgccga
     2701 agccgtacag gagatcaggc gcatcaaggc gcgtgtcgat gccgagcggc tgccgcgcgg
     2761 cgccgatccg aacacacaca ccaagctggg ccgcggcggg ctcgccgaca tcgagtggac
     2821 ggtgcagctg cttcaactcc ggcatgccca caaagtgcct gcgctgcaca gcacttcgac
     2881 actggacacg ctcgacgcca tcggaagagc cgagctgatt gccgagggtg acgtcgagct
     2941 gctgcggcag gcgtggttga cggcgaccag ggcgcgcaac gcgctggtgc tggtgcgcgg
     3001 caagccaacc gatcagctgc ccggccccgg ccgccagctc aatgccgtcg cgttggctgc
     3061 gggctgggac tccgacgacg gcggcgagtt cctggacaac tatctgcggg tgacccgccg
     3121 ggcaaaggcg gtagtacgga aggtgttcgg cgaatgagca tggacttcgg gttcgatgtg
     3181 atcagtgcgg aggagtacga ccgtcagcga gtgctctacg aaccgctgac ggccgcgatc
     3241 cgccgactca tcgatgcggg cattcaca
//
LOCUS       DASRJA010000279         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4055759,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000279 DASRJA010000000
VERSION     DASRJA010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4055759"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VFC01_12280"
     CDS             complement(<1..836)
                     /locus_tag="VFC01_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005100453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN80449.1"
                     /translation="MTTAVVVGSGPNGLAAGVALAKEGVQVTVVEATDEIGGGARSSE
                     AILPGLLHDHCSAFHPMAVGSTFLNSLGLERYGLQWRFPKIDCAHPLDSGDAGVLWRS
                     LDDTADGLGSDGPRWRSIFGKPSATFDVLSEDIMGPLLRVPKHPLALARFGLPTVLPA
                     STFAKVFRTERARALFGGVAAHTFRPLHYPMTSAIGLGIITAGHRHGWPVAAGGSQSI
                     TNALALLLGDLGGKIETGVRIESASQLPGADVTMFDVAPGAVNDILGERLPARVARGL
                     KR"
     gene            complement(833..2620)
                     /locus_tag="VFC01_12285"
     CDS             complement(833..2620)
                     /locus_tag="VFC01_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HZN80450.1"
                     /translation="MGFTEPNFPRVEPEAFLNAPFLERVKVLALNWVENGYGVPRMVH
                     AIYIVKLVFFYALGGIVLATVTSDLPAFWHVSEWWNQPIVYQKVILWTVLMEAIGVGG
                     SWGPLAGKTKPMMGGALYWARPGTIRLRPWRRVPFTSGYRRTWFDVALYVALIVSVIV
                     PLVLPGVPSESLSAALPDNTSGLVNPVLLIAPMVLLVLIGLRDKTIFLAARGEQYLPA
                     LLFFTGLPFLDMIIALKLLIVVVWVAAGVSKFGLHFTHVIPPMVSNSPTMPFKWLKRA
                     HYRNYPNDLRPSHLATFMAHVPGTVVEIVAPVALLLSPWPWLTVIAVAIMVAFHLFII
                     STFPMAVPLEWNVLFAYATIFLFLGFPNWNSYGVADMSSPWLTVAVVAGLLFFPVLGN
                     LRPDLVSFLPAMRQYAGNWASATWAFAPGAEEKLNRVTRSAGNQVDQLVAFGYEPQWA
                     EIFAQQPIGWRSMHSQGRGLFSVLVKNLPDIDTRTVREAEVVCNTLIGFNFGDGHLHN
                     EDLIAAVQDQAQFAPGELVVVWVESQAIATSKVQHYKVIDAALGVIERGTWNVADAVA
                     EQPWLPNGPIPLHVTWTRPLTDPRFGAAA"
     gene            complement(2677..>3661)
                     /locus_tag="VFC01_12290"
     CDS             complement(2677..>3661)
                     /locus_tag="VFC01_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN80451.1"
                     /translation="HANLNAMCRMAIDAFKMTSEDHSLLILPLFHANGIVVGTLSPLL
                     AGGRATVAGRFNPKTFFDRLKRSRATYFSAVPAMYTMLADLPADVRPDTTALRFAICG
                     AAPASVELLTKIETRYGIPLIEGYGLSEGSCASTANPLEGKRKVGTVGPPLPGQKIRI
                     VDADNEDVPPGESGEVLVQGPNVMRGYLNRPEETAKTLVDSWLHTGDIGRFDEDGYLV
                     LVDRAKDMIIRGGENIYPKEIETVVYQLPEIAEAAVVGRAHPVYGEEPLLFVSLNSGA
                     AASTDGIVEHLSGSLSKFKLPVEITILNDLPKNPVGKIDKPTLRKSLAAIH"
BASE COUNT          712 a         1162 c         1170 g          617 t
ORIGIN      
        1 aaacgcttga ggccccgcgc gacccgggcc ggtagacgtt cgcccaggat gtcgttgact
       61 gcgcccggtg cgacgtcgaa catcgtcacg tccgctcccg gcaactgtga ggccgattcg
      121 atgcgcacgc ctgtctcgat cttgccgccg agatcaccga gcagcaatgc cagcgcgttg
      181 gtgatcgatt gtgaaccgcc tgccgcgacc ggccagccgt gacgatgtcc ggcagtgatg
      241 atcccgagcc cgatcgcgga ggtcatcggg tagtgaagcg gcctaaacgt gtgtgcggca
      301 acaccaccga acaatgcccg cgcgcgttct gtgcggaaga ccttggcgaa tgtcgaggcg
      361 ggaagcaccg tgggcagccc gaaccgtgcc agcgcgagcg gatgtttggg cactcgcagc
      421 agcggaccca ttatgtcctc ggagagcaca tcgaaagtcg ctgagggctt tccgaagatc
      481 gaccgccacc ggggaccatc gctgccgagg ccgtcggcgg tgtcatcgag cgagcgccac
      541 agcacgcctg cgtcgccgct gtcgagcgga tgggcgcaat cgattttggg aaaccgccac
      601 tgcaagccgt atcgctccag cccgaggctg ttcaggaagg tggaccctac cgccatgggg
      661 tgaaacgccg agcagtggtc gtgcagcagg ccgggcagga tagcctcgct cgaccgagcg
      721 ccgccgccga tctcgtcggt cgcttccacc accgtcacct gcacaccctc tttcgcgagg
      781 gcgacgccgg cggcgagccc gttgggccca ctgccaacca caacggcagt ggtcacgccg
      841 ccgccccgaa tcgcgggtca gttagggggc gggtccacgt cacgtgcagc gggatcggtc
      901 cgttgggcag ccacggttgt tcggccactg cgtcggccac gttccaggtg ccgcgctcga
      961 tcacaccgag cgccgcatcg atgaccttgt agtgctggac cttgcttgta gcgatggcct
     1021 gcgactcgac ccacaccacc acgagttcac cgggtgcgaa ctgagcctgg tcctgcactg
     1081 ctgcgatcag gtcctcgttg tgcagatggc catcaccgaa gttgaagccg atcagggtat
     1141 tgcagaccac ctcagcctca cgcacggttc gcgtgtcgat atccgggagg ttcttcacga
     1201 gcaccgagaa cagacctcgg ccttggctat gcatcgaccg ccagccgatc ggttgttgcg
     1261 cgaagatctc tgcccactgg ggctcgtaac caaacgcgac cagctggtcg acctggtttc
     1321 cggcagagcg cgtcaccctg ttcagctttt cctcggcgcc cggcgcaaac gcccaggtcg
     1381 ccgatgccca gttgcccgcg tactggcgca tcgcgggcag gaaggacacc aggtccgggc
     1441 gcaggttgcc gagcaccggg aagaacagca ggccggccac gacggcgacg gtcagccacg
     1501 gcgaggacat gtcggcgacg ccgtagctgt tccagttcgg gaacccgagg aacaggaaga
     1561 tcgtcgcgta cgcgaagagc acgttccact ccaacggcac cgccatcggg aacgtcgaaa
     1621 tgatgaacag atggaacgcg accatgatcg ccaccgcaat caccgtcagc cagggccagg
     1681 gtgagagcag aagcgcaacg ggggcaacga tttccacgac ggtgcccggc acgtgcgcca
     1741 taaaggtggc gagatgcgac ggacgaaggt cgttcgggta gttgcggtag tgggctcgct
     1801 tgagccactt gaacggcatt gtcgggctat tgctcaccat cggcgggatg acatgggtga
     1861 agtgcaggcc aaatttcgag acaccggcag cgacccagac gacgacgatc agaagcttga
     1921 gcgcgatgat catgtcgagg aacggcagcc cggtgaagaa caacaacgcc ggcaggtact
     1981 gctcaccacg cgcggcaagg aagatggtct tgtcgcgcag cccgatcaag accagcagca
     2041 ccatcggggc aatcagcaac acgggattca ccagaccgga tgtgttgtcc ggcaacgctg
     2101 ccgacagcga ttcactggga acgccgggca gcaccagagg aacgatcacg ctgacgatca
     2161 gcgccacata gagcgcgacg tcgaaccacg tccgccggta accggaagtg aagggaaccc
     2221 gccgccaggg acgcaatctg atcgtgccgg gccgcgccca ataaagagcc ccacccatca
     2281 tcggtttggt cttaccggcc agcggacccc aagacccacc gacgccgatg gcctccatca
     2341 gcaccgtcca cagaattacc ttctggtaga cgatcggctg gttccaccac tccgaaacat
     2401 gccagaacgc cggcaggtcg gaggtgacgg tggctagcac gatgccgccc agtgcgtaga
     2461 agaacacgag cttgacgatg tagatcgcgt gcaccatccg gggcacgccg tacccgtttt
     2521 ctacccagtt cagggctagg accttcacgc gttccaggaa cggtgcgttt agaaaggctt
     2581 cgggctcaac ccgcgggaag ttcggttcgg tgaatcccat gatcgactcc ttctggatag
     2641 ttcaggctag gtctgcggtt gtctggccgt ggatgatcag tggatcgctg cgagcgattt
     2701 ccgcagcgtg ggtttgtcga tcttgccgac cgggttctta ggcaggtcgt tgagaatcgt
     2761 gatttcaacg gggagtttga atttcgacaa cgacccgctg aggtgctcga cgatgccgtc
     2821 ggtggaggca gcggcgcccg agttcaggga cacgaacagg agcggctcct cgccgtacac
     2881 cgggtgggcg cgaccgacca cggcggcctc ggcgatctcc ggcagttggt agacgactgt
     2941 ctcgatctcc ttcggataga tgttctcgcc gccgcgaatg atcatgtcct tcgctcgatc
     3001 gacgagcacc aggtagccgt cctcgtcgaa ccgtccgatg tcgccggtat gcagccagct
     3061 gtcaacaagc gtcttcgccg tctcctccgg ccggttcagg tacccgcgca tgacgttggg
     3121 tccctgaacc aggacctcgc cggactcacc cggcggaacg tcctcgttgt cggcgtcgac
     3181 gatccgaatc ttctgccccg gcaacggggg cccaacggta cccaccttgc gtttaccctc
     3241 gagcgggttc gcggtgctgg cgcaggatcc ctcggacaac ccgtagccct cgataagggg
     3301 gatgccgtag cgggtctcga tcttggtcag cagctcgacg cttgccggtg cggcaccaca
     3361 gatggcgaac cggagggctg tcgtgtccgg ccgcacatcg gcaggcaggt cggcgagcat
     3421 cgtgtacata gccggaaccg cggagaagta ggtcgcgcgg ctccgcttca gccgatcgaa
     3481 gaacgttttc gggttaaacc ggccggccac ggtagcgcga ccgcccgcca gcagcggcga
     3541 caacgtgccg accacgatcc cgttcgcgtg aaacaacggc aagataagca ggctgtggtc
     3601 ctcgcttgtc attttgaacg cgtcgatcgc catgcggcac atggcattca ggttggcgtg
     3661 a
//
LOCUS       DASRJA010000280         6823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_690160,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000280 DASRJA010000000
VERSION     DASRJA010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690160"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFC01_12295"
     CDS             <1..627
                     /locus_tag="VFC01_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZN80452.1"
                     /translation="NLVGAFHQPAAVLIDLATLETLPRNEIVAGMAEIVKAGFIADPV
                     ILDLIEADPEAALDPTGTVLPELIRRAVAVKAEVVAADEKESQLREILNYGHTLAHAI
                     ERRERYQWRHGAAVSVGLVFAAELGRLAGRLDDETADRHRSVLTSLGLPVTYDADALP
                     QLLEYMAGDKKTRAGVLRFVVLDGLSKPGRLEGPDPSLLAAAYAEVAQ"
     gene            624..1085
                     /gene="aroQ"
                     /locus_tag="VFC01_12300"
     CDS             624..1085
                     /gene="aroQ"
                     /locus_tag="VFC01_12300"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HZN80453.1"
                     /translation="MTTVLVLNGPNLGRLGRREPEVYGSTTHDDLVALIEREAGELGL
                     KAVVRQSDSEADLLGWVHAAVDAGDPVILNAGALTHTSVALRDACAELRAPLIEVHIS
                     NVHAREEFRHHSYISAVATGVIIGLGVQGYVLALRYLASLGEERPPVPAAE"
     gene            complement(1097..1693)
                     /locus_tag="VFC01_12305"
     CDS             complement(1097..1693)
                     /locus_tag="VFC01_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B-4DMT family transporter"
                     /protein_id="HZN80454.1"
                     /translation="MSKWLLRGLVFAALMVIVRLLQGALINAWETKAGLISIVLVVLY
                     AVVVFIWGLSDGRSDARANPDPDRRGDLAMTWLLAGLFAGVVSGAVTWFISLFYKSLY
                     VEALLNELTTFAAFTALLVFLFAVAGVALGRYLVDRKLEKQPRKHHGLAEAAADQDQD
                     RADTDVFAAVRHPGADERTERTESTEQTAPTQARREER"
     gene            1727..2812
                     /locus_tag="VFC01_12310"
     CDS             1727..2812
                     /locus_tag="VFC01_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZN80455.1"
                     /translation="MTISQRRDRLRQRLIDAELDAMLVSDLVNVRYLSGFTGSNAALL
                     VLADRETPVLATDGRYRTQAAQQAPDAEIVIERACGPHLAGRAAEDGARRVGFESHVV
                     TVDGFAALRKAAGDSTELVRAAGTVEALREVKDAGEVALLRLACEAADAALTDLVERG
                     GLSAGRTEKEVARELESLMLDHGADGVSFETIVAAGANSAIPHHRPTDAALAAGDFVK
                     IDFGALVCGYHSDMTRTFVLAPAAQWQLDIYQLVAAAQQAGRDALAPGVTLKDVDGAS
                     RQVIAEAGYADNFSHGLGHGVGLQIHEAPGISASAAGTLLAGSTVTVEPGVYLPDQGG
                     VRIEDTLVVCDKTPDLLTRFPKELAIL"
     gene            2827..3390
                     /gene="efp"
                     /locus_tag="VFC01_12315"
     CDS             2827..3390
                     /gene="efp"
                     /locus_tag="VFC01_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZN80456.1"
                     /translation="MATTADFKNGLVLQIDGQLWQIVEFQHVKPGKGPAFVRTKLKNV
                     LSGKVVDKTYNAGVKVETATVDRRDATYLYRDGSDFVFMDSADYEQHHLSDSLVGNAA
                     KFLLESLPVQIAFHDGAPLYLELPVTVELEVTHTEPGLQGDRSSAGTKPATVETGAEI
                     QVPLFINTGDKLKVDSRDGSYLGRVNA"
     gene            3383..3862
                     /gene="nusB"
                     /locus_tag="VFC01_12320"
     CDS             3383..3862
                     /gene="nusB"
                     /locus_tag="VFC01_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZN80457.1"
                     /translation="MPDRKGDRGRHQARKRAVDLLFEAEARGITPAEVAESRNALAEK
                     ESDVSPLNAYTVTVARGVTEHAAHIDDLISAHLQGWTLDRLPAVDRAILRVAVWELLH
                     ADDVPEPVAVDEAVELAKQLSTDDSPGFVNGVLGQVMLVTPQIRAASAAMRGSSEGT"
     gene            3863..4405
                     /locus_tag="VFC01_12325"
     CDS             3863..4405
                     /locus_tag="VFC01_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80458.1"
                     /translation="MGVPQDWAPYSSVEEAARVYLRDPELALDQLRSVIDLAAIKSFI
                     MSRGSSEESWGEALWQEVVLTDGQRLIMWRADDELSTAEDRERRVLNASVRTILLSTI
                     TDHVLTAEYEVLSDDTRRLSEVRLRMYTQLVTRSRQKSATETDIFCESFRYYKSVDNG
                     GLAQMQRLLQFGRVLSRCMQ"
     gene            4504..5598
                     /locus_tag="VFC01_12330"
     CDS             4504..5598
                     /locus_tag="VFC01_12330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZN80459.1"
                     /translation="MTTTALTVWAVNPPIRLAGSDGKTHLEYDLILQSMFNAPVTVSS
                     IEVLAPDGASLLKLDGDDVAKFTTPAFIGPPTAVVPPSGALGTVIDVVVPTDKVPDRL
                     NHRISYRPGTSDVGSIVGTSDIVGPELDVDPRPPVVIASPLKGQTWLNGNSCCVPESP
                     HRRGRIAVDGNSIKKFEEFAVDWVQLRDGKLFDGDGSRNEHWYGFGADVVAVADGSVA
                     AVFDGMGEQTPNTPAVGLRGPRDYSGNHVSLQISPGVWAAYAHLQPGSITVKTGDEVK
                     KGQVIGKLGNTGNSSAPHLHFQLSDGPEITTSNSLPFSLESYQLAGAVDPAQFNDAAD
                     GKGLPPPLRVIGPGRQESGTFPLMYAVTDF"
     gene            5754..6641
                     /locus_tag="VFC01_12335"
     CDS             5754..6641
                     /locus_tag="VFC01_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HZN80460.1"
                     /translation="MQPELEQLFDKQGGVATSCQILAHISRRRFEAAINCGVLERIWQ
                     GIYCCGEPTEELRLRGLDLSSGNTVPVCLGTAAAWHGFDTEDSVDLHVLNPPGGQLRC
                     ADGLVVHRRDSAPLVMVNGRPVTSPAWTAVEVARGLRRPRALATLDAAMRSETCSRSE
                     LWRSAVEQAGRRGIVAVRELIPLADALAESPMESEARLAMIDGGLPMPELQYEIIDGN
                     RELRRVDFAWPKARLAVEYDGVDWHSDPDAMKRDRRRRAALEAVGWVVISIVFEDVRY
                     RAWEFVARIDAQLRHARAA"
BASE COUNT         1188 a         2170 c         2335 g         1130 t
ORIGIN      
        1 aacctggtcg gtgcgtttca tcaaccggct gccgtgctca tcgatctggc caccctggaa
       61 accctgccgc gcaacgagat cgtcgccggc atggccgaaa ttgttaaggc cggcttcatc
      121 gccgaccccg tgattctcga tctgatcgag gccgaccccg aagccgcgct cgacccgacg
      181 ggcactgtgt tgccggagct gattcggcgc gcggtcgcgg tcaaggccga ggtcgtcgcc
      241 gccgacgaga aagaatcgca gctgcgcgag atcctcaact acgggcacac gctcgcgcac
      301 gcgatcgaac ggcgcgagcg ctatcagtgg cgacacggtg ccgcggtgtc ggttggcctg
      361 gtgttcgcgg ccgaactcgg caggctggca ggacgtctcg atgacgagac cgccgaccgg
      421 caccggtcgg ttctgacctc gctgggcttg ccggtgacct acgacgccga cgctctaccg
      481 caactgctgg aatacatggc cggcgacaag aagacgcgtg ctggcgtgct gcgatttgtg
      541 gtactcgacg ggctgtccaa gcccggacgt ctggaagggc cggatccgtc gctgctggcg
      601 gcggcttacg cggaggtggc gcagtgacaa ccgtgcttgt gctcaatggt cccaacctgg
      661 gtcggcttgg gcgacgcgag cctgaggtct atggcagtac gacgcacgac gacctcgtgg
      721 cgttgatcga acgggaagcc ggcgaactag gtctgaaagc cgttgtacgc caaagcgaca
      781 gcgaagccga cctgctgggc tgggttcacg cggctgttga tgcgggcgat ccggtgattc
      841 taaacgccgg tgcgctgacc cacacgtcgg tggcgttgcg cgacgcgtgc gccgagttgc
      901 gggcgccgct gatcgaggtg cacatctcca atgtgcatgc tcgcgaagag tttcggcatc
      961 actcctacat cagcgcggtg gccaccggtg tgatcatcgg cctcggagtg cagggctacg
     1021 tgttggcgct gcggtaccta gcgagcctgg gcgaggaacg accgccggtg ccggccgccg
     1081 aatagcagtc aggccgctac cgctcctcgc gacgcgcttg ggtcggcgcg gtctgctcgg
     1141 tgctctcggt gcgctcggtg cgctcgtccg cgccgggatg gcgcacggcg gcgaacacgt
     1201 cggtgtcggc gcggtcttgg tcttggtcgg cggcggcctc ggcgaggccg tgatgcttac
     1261 gcggctgctt ttcgagtttg cggtcgacca gatagcggcc gagcgcgacg cccgcgaccg
     1321 cgaacaggaa gaccagcagc gcggtgaacg ccgcgaacgt ggtgagctcg ttgagcagcg
     1381 cctcgacgta gaggctcttg tagaacagcg agatgaacca cgtcaccgca ccgctcacca
     1441 caccggcgaa caacccggcc agcagccacg tcatcgccag atcgccgcgg cggtcagggt
     1501 cggggttggc ccgcgcgtcg gagcggccgt cgctcagtcc ccagatgaac accacgaccg
     1561 cgtagagaac gaccagcacg atgctgatca atccggcctt ggtctcccac gcgttgatca
     1621 acgccccctg cagcaaccgc acgatcacca tcagggccgc gaacaccagt ccgcgcagta
     1681 accacttact catggggcca caccttagcg agtaggttca tgaactgtga ctatttcaca
     1741 gcgccgggac cggctgcgcc agcggttgat cgacgccgaa ctggacgcga tgctggtatc
     1801 cgacctggtc aacgtgcgtt atctgtcggg attcacgggt tccaacgcgg cgctgctggt
     1861 ccttgccgac cgcgaaactc cggtgctggc caccgacggt cgctatcgca ctcaggccgc
     1921 gcagcaggcc ccggatgcgg agatcgtcat cgaacgggcc tgcgggccgc atctggccgg
     1981 tcgcgccgcc gaggacggtg cacggcgggt ggggttcgaa agccatgtgg tgaccgtcga
     2041 cgggttcgcc gcactgcgca aggcggctgg ggattccacc gagcttgttc gggcagcggg
     2101 aacggtggag gcgctgcgtg aggtcaagga cgcgggcgag gtcgcgctgt tgcggctggc
     2161 gtgcgaggcg gccgacgccg cgctgacgga tctcgtcgag cgcggcgggc tgagcgcagg
     2221 ccggacggaa aaagaggtgg cgcgcgaact cgaatcgctc atgctcgacc acggcgcgga
     2281 cggcgtgtcg ttcgaaacga tcgtggcggc gggggccaac tcggcgatcc cgcaccacag
     2341 gccgaccgac gcggcgctgg cggccggcga cttcgtcaag atcgacttcg gtgcgctggt
     2401 gtgcggttat cactccgaca tgacccgcac ctttgtgttg gcgccggccg cgcagtggca
     2461 gctcgacatc tatcagctgg tggctgcggc gcaacaagcc ggccgtgacg cgctcgcgcc
     2521 cggcgtcacg ctcaaggatg tagacggcgc gtcgcggcag gtcatcgccg aagcgggcta
     2581 cgccgacaac ttcagccacg ggctcggcca tggtgtcggg ctgcagatcc acgaagcgcc
     2641 gggaatcagc gcgtcagccg ccggtacact gcttgctggc tctacggtca ccgtcgagcc
     2701 aggtgtctat ctgcccgacc agggcggcgt ccgaatcgag gacacgctgg tcgtgtgcga
     2761 caagaccccc gacttactca cccggttccc caaagaactg gcgatcctct aggagaacga
     2821 ccgaacgtgg caacgaccgc cgacttcaag aacgggctcg tcctgcagat cgacggccaa
     2881 ctgtggcaga tcgtcgagtt ccagcacgtc aagcccggca agggaccggc cttcgtgcgg
     2941 accaagctga agaacgtgct gtccggcaag gtcgtcgaca agacctacaa cgccggcgtg
     3001 aaggtggaga ccgcgaccgt cgaccggcgc gacgcgacct acctctaccg cgatggctcc
     3061 gacttcgtgt tcatggactc ggcggattac gagcagcatc acctctcgga ttcactggtc
     3121 ggtaacgccg ccaagttcct gctggagagc ctgccggtgc agatcgcgtt ccacgacggc
     3181 gcaccgctgt acctcgagct gccggtgacc gtcgaactcg aggtcacgca caccgagccg
     3241 ggcctgcagg gcgaccggtc cagcgcaggc accaaaccgg ccaccgtcga aaccggcgcc
     3301 gagatccagg tgccgctgtt catcaacacc ggcgacaagc tgaaggtcga ctcccgcgac
     3361 ggcagctact tgggccgggt gaatgcctga ccggaagggt gatcgcggcc ggcaccaggc
     3421 ccgcaaacgc gccgtcgacc tgctgttcga ggccgaggcg cgggggatca cccccgctga
     3481 agtcgccgag tcgcgaaatg ccttggcgga gaaggaatcc gacgtctcgc cgctgaacgc
     3541 ctacacggtg acggtggcac gcggagtgac cgagcacgcc gcgcacatcg acgacctcat
     3601 ctcggcgcac ctgcagggct ggacgctgga caggctgccc gccgtcgacc gggcgatcct
     3661 gcgggtggcg gtgtgggaac tgctgcacgc cgacgacgtt cccgaaccgg tcgccgtcga
     3721 cgaggcggtg gagctggcca agcagctgtc caccgacgac tcacctggtt tcgtcaacgg
     3781 tgtgctgggt caggtgatgt tagtgacgcc gcagatccgg gcggcgtcgg cagcgatgcg
     3841 gggtagttcg gaagggacat agatgggcgt tccgcaggac tgggcaccgt actccagtgt
     3901 cgaggaggcg gccagggtat acctgcgtga tcccgaattg gccctggacc aactgcgttc
     3961 ggtcatcgac cttgctgcga tcaagtcctt catcatgtcg cgcggatcca gcgaggagtc
     4021 gtggggcgaa gcgctgtggc aagaggtcgt gctgaccgac ggccagcgtc tgatcatgtg
     4081 gcgcgccgac gacgagctgt ccacagccga agatcgcgaa cggcgcgtgc tgaacgcgtc
     4141 ggtccgaaca atcctgttgt cgacgatcac cgaccacgtc ttgacggccg agtacgaggt
     4201 gctgagcgac gacacccgcc gactctccga agtgcgactg cggatgtaca cccaactcgt
     4261 cacgcggtcg cggcagaagt cggccaccga aaccgacatc ttctgcgagt ctttccgcta
     4321 ctacaagtcc gtcgacaacg gcggcctggc tcagatgcaa cgcctgctgc agttcggccg
     4381 ggtgctgtcg cgatgtatgc agtaattcgc gtcgcggtct gtgccgtggc gctggcagcg
     4441 tgttcgactt ctgatcccac gccggcgccg tcatcaccgg tgacgcctca gccgcaggcg
     4501 gaagtgacga caacggcgct gacggtgtgg gcggtcaatc cgccgatccg gctcgccggc
     4561 agcgacggca agactcatct cgagtacgac ctgatcctgc agagcatgtt caacgcgccg
     4621 gtaacggtct cgtcgatcga ggtgctggca ccggacgggg catcgctgtt gaagctcgac
     4681 ggcgatgacg tcgccaagtt caccacgccc gcgttcatcg gaccgccgac cgccgtggtg
     4741 ccgccgagcg gagcgctggg cacggtgatc gacgttgtgg tgccgacaga caaggtgccc
     4801 gaccgactca accatcgaat cagttaccgc ccaggcacat cggatgtcgg atcgatcgtg
     4861 ggcacgagcg acatcgtcgg cccggagctt gacgtcgacc cgcgcccacc ggttgtcatc
     4921 gcgtcgccgc tcaagggtca aacctggcta aacggcaact cctgctgcgt gccggaatcg
     4981 ccgcatcggc gcgggcgaat cgcggtggac ggcaacagca tcaagaagtt cgaggagttc
     5041 gcggtcgact gggtgcagct gcgtgatggc aagttgttcg acggtgatgg cagccgaaac
     5101 gagcactggt atggcttcgg cgccgacgtg gtcgcggtcg ccgacggctc cgtcgccgcg
     5161 gtattcgacg ggatgggtga acagacgccg aacacgccgg cggtcggact gcgcgggcca
     5221 cgggattaca gtggaaacca tgtgtccctt cagatttcgc ccggcgtctg ggccgcctac
     5281 gcgcatctgc agccgggcag catcacggtc aagaccggtg atgaggtgaa gaagggtcag
     5341 gtgatcggca agctgggcaa caccggaaac tcgagcgctc cgcacctgca cttccagctg
     5401 tccgatgggc cggagatcac gacgtcgaac agcctgccgt tctcgctcga gtcgtaccag
     5461 cttgctggcg cggtcgatcc ggcacagttc aacgacgcgg ctgacggcaa gggtttaccg
     5521 ccgccgttgc gcgtgatcgg cccgggccgc caggagtccg gcacctttcc attgatgtac
     5581 gcggtcaccg acttctaact gccgcgggtg tgcgtgtctg tacgccgaca cgccgcaata
     5641 tgcgtacatt tcgcgcaccc tcgcgggcgc cgactgtgca cagccgctca ttcatccaca
     5701 gataggtcgt caggggctac cgcgtcgacg ggccggcttc gatgatccac gctatgcaac
     5761 ctgaactcga gcagctcttc gacaagcaag gcggcgtagc cacgagttgc caaatcctgg
     5821 ctcatataag cagacgtcgt ttcgaggcgg ccatcaattg cggtgtcctg gaacgcatat
     5881 ggcagggcat ctattgctgc ggcgagccaa cggaggagct gcgcctgcgt gggcttgatc
     5941 tgtcgagcgg caacacggtg ccggtgtgcc tcggcaccgc cgctgcgtgg cacggcttcg
     6001 acacggagga ctccgtggac ttgcacgtcc tgaatccgcc cggcggccag ctccgatgcg
     6061 ccgacggcct ggttgtgcat cgccgcgaca gcgctccgct ggtaatggtg aacggacgac
     6121 ccgtgacgtc tcctgcttgg accgcagtcg aggttgctcg cgggctgcgc cggccgcggg
     6181 cgctggcgac actggacgcg gcgatgcgca gtgaaacgtg tagtcgcagt gaactctggc
     6241 ggtcggcggt tgagcaggcc ggtcggcgtg gcatcgtcgc ggtgcgcgaa ctgatcccgc
     6301 tcgccgacgc gctggcggag tcgccgatgg aaagcgaagc gcgcctggcg atgattgacg
     6361 gcggcttgcc gatgccggaa ctgcagtacg agatcatcga cggcaatcga gagctcaggc
     6421 gcgtcgactt cgcttggccg aaggcgcggc tcgccgtcga gtatgacggg gtcgattggc
     6481 acagcgatcc ggacgccatg aagcgggatc ggaggcgacg tgcagcgctc gaggcagtcg
     6541 ggtgggtcgt catctcgatc gtgttcgagg acgtccgata ccgggcgtgg gagttcgtcg
     6601 ctcgcatcga tgcgcagttg cgtcacgctc gcgccgcttg agcgtgcgcc gaatgcacgc
     6661 agattgcggc gtgtcggcgt acagacacgc acgctcggcg tagtgggcga gagagttgcg
     6721 gtcagctcag ttgactgacg atcgccgcgt tgtccagcca gacgttctcg cggctgacga
     6781 gcccgtcccg gaactcgaag acgtgcaaga tccggaagct gat
//
LOCUS       DASRJA010000281        13538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1694598,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000281 DASRJA010000000
VERSION     DASRJA010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13538
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694598"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFC01_12340"
     CDS             <1..385
                     /locus_tag="VFC01_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN80461.1"
                     /translation="NTAAWARALGRRVCAVPGPVTSSASVGCHVLLRAGAHLVTRADE
                     VLELVGHAGELAADVERPASALDELGDSELRVYDALPARGARTADEIAVASGLPPTQV
                     LGPLSMLEIAGLVVRQGGRWKLARR"
     gene            492..1340
                     /locus_tag="VFC01_12345"
     CDS             492..1340
                     /locus_tag="VFC01_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HZN80462.1"
                     /translation="MAGRPVHTFEVVRSEQLTPHLTRVVLGGEGFDTFTPNEYTDAYV
                     KIVFVRDDVDVGALPQPLTLDSFNELPVEQRPTVRTYTVRRVDADRREIAIDFVVHGE
                     HGVAGPWAAAATPGQPAYLTSPNGAYAPDPAADWHLFAGDEAAVPAIGAALEALPDNA
                     IGKVFIEVAGPEDEIPLSAPEGVEVRWIYRGGRADLVPEDVAGDNAPLISAVKEADWL
                     PGQVQVFIHGEAQTVMHNLRRYIRKERGVDAKWAASISGYWRRGRTEETFRQWKRELA
                     EAEATA"
     gene            complement(1347..3122)
                     /locus_tag="VFC01_12350"
     CDS             complement(1347..3122)
                     /locus_tag="VFC01_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005197493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN80463.1"
                     /translation="MAEFLSRRDVDFLLYEWLDVVELTKRDHFAEHSRDTFDAVLDLS
                     VDIATKCFAPHNKKGDQNEPTVGPDGKVVLIDEIKDALDTFTGAGLVAGSFDEAVGGM
                     QLPTVLAQASMAFFRAANPSTTSYAFLTVGNANLLVEYGTEEQIDTWVRPMLEGRYFG
                     TMCLSEPDAGSSLTDITTKAVPAADGTYRITGTKMWITGGDHELTENIVHLVLAKAPG
                     GGPGVKGISLFIVPKFLADGTRNDVALVSLNHKMGNHSTTNALLNFGDGTHQPDAEPG
                     AVGYLVGEEHKGLAYMFHMMNEARMAVGLQATATGYAGYLASVEYAKERTQGRPVGDK
                     DPGRPQVALIEHADVKRMLLAQKSYVEGALALGLYCAMLVDEGRTSVGAERDRANLLL
                     EVLTPIAKSFPSQWCVEANSLAIQVHGGYGYTKEFDVEQCYRDNRLNSIHEGTHGIHG
                     LDLLGRKVVMQDGAGLTLLAEKINATIDKALASDSTAAYAQQLREAVQRLVDITGKLW
                     AAGDVATTLANSTVYLEAAGHVVVAWMWLEQLIAASDHQGDFYDGKRAAAQYFYTFEL
                     PKVGPQFDLLASLDRTTVDAQASWF"
     gene            3233..3817
                     /locus_tag="VFC01_12355"
     CDS             3233..3817
                     /locus_tag="VFC01_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80464.1"
                     /translation="MTIDRAADSAAAERMRRAAIEAFAETGYGGSSTRQIAKRLNMSA
                     TAMYPHYRSKEELLYAIALEGHSSLLAALKRADDLAASFTERLRAVVAAFASWHAENH
                     KQARVVQYELHGLSPAHYRTIVPLRHQATDVITEIVAGGVTAGEFDVDNVDELVMAIS
                     SLCVDVCRWFPSRQHRDAQKLGEVYAGVALRMVR"
     gene            3851..5011
                     /locus_tag="VFC01_12360"
     CDS             3851..5011
                     /locus_tag="VFC01_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HZN80465.1"
                     /translation="MAFGDYQLEIYLQGLSGIVPSLPMSYAEWEAKAEAAMPPSIWSY
                     VAGGAGDERTQRVNRTAFDGWGLMPRMFVGAKERDLSVEVFGLTVPSPVFMAPIGVTG
                     LCAQDGHGDLATARAAARTGVPMVISTLTEDPMEEIAAEFGDTPGFFQLYTPTDRDLA
                     ESFVRRAEAAGYKGIIVTLDTWVPGWRPRDLSTSNFPQLRGRCLANYTSDPVFRASLQ
                     QPPEENMQAAVLRWVSVFGNPLTWDDLPWLRSLTDLPLIVKGICHPDDARRARDGGVD
                     GIYCSNHGGRQANGGIPAIDCLRAVVSAADGLPVLFDSGIRSGADIVKALALGATAVG
                     IGRPYAYGLAIGGEDGVVHVLRSLLAEADLIMAVDGYPTLKDLTPEALRRVT"
     gene            5044..5946
                     /locus_tag="VFC01_12365"
     CDS             5044..5946
                     /locus_tag="VFC01_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HZN80466.1"
                     /translation="MQAIFEEFDEYLALQRGRSEHTRRAYLGDLRSLFDFLAERMPGA
                     GLDRLSLPILRSWLSAQASAGAARTTLARRTSAVKTFTAWATRRGLLSTDPAARLQMP
                     KARRTLPSVLRQDQALDAMAAAKSGAQQGDPLALRDLLVVELLYATGIRVSELCGLDI
                     DDVDRPRRLLRVLGKGNKQRTVPFGEPAQAALTAWLDDGRPELATADSGPALLLGARG
                     RRLDPRQARTVVHQTVAAVDGAPDIGPHGLRHSAATHLLEGGADLRVVQELLGHSTLA
                     TTQLYTHVTVRRLRAVHDQAHPRA"
     gene            complement(6144..7616)
                     /locus_tag="VFC01_12370"
     CDS             complement(6144..7616)
                     /locus_tag="VFC01_12370"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZN80467.1"
                     /translation="MAVMSAATTPLPSVPTDLLIGGRWQPAACARSISVVNPATEDVL
                     ARVADADEVDGLAALAAAAESQHDWASTPAGRRADILDRAYRAILTRRGEFAALIAAE
                     MGKPWSEAQSEVDYGAGFFRWFAESARRLHLDGQFGIEPGGQYRIAVSKRPVGPSLLI
                     TPWNFPLAMGARKVAPALAAGCTTILKPAEQTPLSSLLLAQVLEDCGVPAGVVNVITT
                     TRSSEVVAAIMRDCRARKVSFTGSTRVGKILLRQAAENVMNTSMELGGNGPLIVFADA
                     DLDIAVEGALVAKLRNGGESCVAANRIFVQREIAATFTERFTERMRNVAVGDASNAGT
                     AIGPLIDERQLHKVSDLVHDAVAKGAHCLTGGHRVEREGFFYAPTVLHNIPDDAAICT
                     EEIFGPVAPIYEFDSEEQVLAAANATEYGLASYVFTRDLSRALKVADALDTGMTGINR
                     GVVSNPVAPFGGIKASGIGREGGIEGLDAYLETKYTGIEL"
     gene            complement(7859..8545)
                     /locus_tag="VFC01_12375"
     CDS             complement(7859..8545)
                     /locus_tag="VFC01_12375"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN80468.1"
                     /translation="MTKAVADHIAIVGKSTAQQVADGLTSMIIEGAISPGERISEAGI
                     ARSLGLSRNTVREAVRLLQGSGLVRYNFNRGMEVWDPTDDDVVDVFKARLYLERLAAQ
                     SLTAESDLAPMRDAHRRYVQALETHDLRTIVDCDLAIHQAAVGILGSARIDQFYAALL
                     NELRYFLLVLSYDRNEYLDNEGVAHEHQEMVEAFESRDPARAIQVVTAILEENRDLAR
                     GITAQRKKTD"
     gene            8680..10134
                     /locus_tag="VFC01_12380"
     CDS             8680..10134
                     /locus_tag="VFC01_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014011416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN80469.1"
                     /translation="MQEEQAAQADGSAPKLIVHGELKRNSLRNRHLTMIALGGIIGAS
                     LFVGSGSVIASAGPAALLCYAIGGVLVVLVMRMLGEMAVARPALGSFMEYAREALGDR
                     AGFIVGWLYWYFWVGVVAFEAVVGGRIVSGWTGAPAWVFALGLMFVLTVSNLLSVKSF
                     GETEFWLASIKIATIIVFLIVGSLWVLGVFPHSHFSVPNIAYGGFFAHGAMPVLQGVV
                     TVIFAYFGTEIVTMAAAESRQPARAIARATNTIVWRIMLFYVGSVALLLMITPWPEIP
                     STSSPFAAAFDLFGFPAAATIINVVVLTAALSVLNSGLYTASRMVYALGGTGWAPGWV
                     TDTNRRGVPWKAILLSTVVGYVAIVMNYVSPDTIFAFIMNSAGAVALFVYLIIAFSQL
                     RLRSQLTKQGAFDSQLKMWFHPWLGILTIVGICVVLGAMGYLESTRSQLLLSLLSVGA
                     LLVAYPFARRSRERLLASAVPSTIAGSQPNPARS"
     gene            10147..11409
                     /locus_tag="VFC01_12385"
     CDS             10147..11409
                     /locus_tag="VFC01_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012686893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN80470.1"
                     /translation="MTDQDQTSGTALWNAQAHMPSVAERQIVISRGEGSYIYTTDGRR
                     LFDGTASLWFANIGHGRKEMAEAAYRQMLNLETYHVFGRYTTDRSVALADRLASLSPI
                     EDCKVIFTSGGSDSIDTACKLARRHWQLRGRTSKRTILSRQFAYHGLHAYGTSITGLD
                     FNREGYGTESLVPETARVSNADIDAVARVIDEIGAENIAALVFEPIQGTGGVNPPPPG
                     YLEGLERICRDNDILRITDEVITGFGRTGRMFASERYGLQSDLVTFAKGVTSGYAPLG
                     GILIAPSVWEPYFSPQSELTFRHGVTYSGHATACAVAETNLDILEKEHLLERAEKLEL
                     TLHHELDSLSDLDAVEDIRVAGFLGGVSLRADLDAPAIADDLVDRGFIVRALRGNTLQ
                     LSPPLVTTDEEVVQLAAALRESLVDADR"
     gene            complement(11482..12225)
                     /locus_tag="VFC01_12390"
                     /pseudo
     CDS             complement(11482..12225)
                     /locus_tag="VFC01_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213261.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(12249..13214)
                     /locus_tag="VFC01_12395"
     CDS             complement(12249..13214)
                     /locus_tag="VFC01_12395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN80471.1"
                     /translation="MTTDAHRSRPLRFGLHTALPRGAEFGAFANSVQDAGFDVLTIPD
                     HLVASVSPFAGAMAAAVATDRLHTGTLVLNNDLRHPIDTAREAATVAAISGGRFELGL
                     GAGHMKSEYDAAGIRFDSGKTRVDRLIEAVDVIRALLVGEPVDVDGAHYCVRAEAGEL
                     VAAPGVAVPLLVGGNGTRVLQLAGRVADIAGLAGFSHNRDATKVELTHFDAAGLEDRI
                     AVVRDAAGDRFDAIELNALIQFVVHTDDREASAAELAAAFGGISPEFVLDSPFVLLGT
                     HEQMAEALIERQQRFGVSYWTVFDEWVGRDSAMPDIAKVIALLRE"
     gene            complement(13234..>13538)
                     /locus_tag="VFC01_12400"
     CDS             complement(13234..>13538)
                     /locus_tag="VFC01_12400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZN80472.1"
                     /translation="YAAGAGTVVFAGELAGRTLVSIAHSGGLRTTYEPVRSSVRVGQL
                     IDAGAVIGELEAGHGGCAAAACLHWGAMWGPASRADYVDPLGLLASTPVRLKPLGG"
BASE COUNT         2354 a         4378 c         4361 g         2445 t
ORIGIN      
        1 caacaccgcg gcatgggcgc gggcactggg tcgtcgggtg tgcgcggtgc cagggccggt
       61 cacgtcgtcg gcctccgtcg ggtgccatgt gctgctgcgt gccggggcgc acctggtcac
      121 ccgcgccgac gaggtcctgg agctggtcgg gcatgctggc gaactcgctg cggatgtaga
      181 gcgaccggcg tcggcgctgg atgagctcgg cgacagcgag ctgcgtgtct acgacgcact
      241 tcctgctcgc ggcgcgcgta cggccgacga gattgcagtc gcgtccggat tgccaccgac
      301 gcaagtgttg ggtccgctgt cgatgctgga gatcgcgggc ctggtcgtgc gtcaaggtgg
      361 gcgctggaag ctggctagac gataagttgc cacagccagg ggctgacaag gtagcgcgcc
      421 acctcttcgg tcgcgctcgt atcctcgatc aggttggcca acgaagtgtc ggtcgatgat
      481 ttggaggaac agtggcagga cgtccagtgc acacgttcga agtggtgcgc agtgagcagt
      541 tgaccccgca cctcacccgc gtcgtcctgg gcggagaggg gttcgacacg ttcaccccca
      601 acgagtacac cgacgcctac gtcaagatcg tcttcgtcag ggacgacgtc gacgttggcg
      661 cgctgccgca gccgctcaca ctggacagct tcaacgagct acctgtcgag cagcggccca
      721 ccgtgcgcac ctacaccgtg cgccgcgtcg acgccgatcg ccgcgagatc gccatcgact
      781 tcgtcgtgca cggcgagcac ggtgtcgccg gaccgtgggc agcggcggcc accccgggtc
      841 aaccggccta cctgacgagc cccaacggcg cctacgcacc cgatcccgct gccgactggc
      901 acctgttcgc cggtgacgag gcggccgtcc cggccatcgg cgccgcgctg gaggccttgc
      961 ccgacaacgc aattggcaaa gtcttcatcg aggtcgccgg acccgaggac gagatcccgc
     1021 tcagcgcacc tgaaggtgtc gaggtgcgat ggatctaccg cggcggtcgc gccgacctgg
     1081 tgcccgaaga tgttgcgggc gacaacgcac cgctgatctc cgcagtcaag gaagccgact
     1141 ggctgccggg gcaggtgcag gtcttcatcc acggcgaggc ccagaccgtc atgcacaacc
     1201 tgcggcgcta catccgcaag gagcgcggtg tcgacgcaaa gtgggccgcg tcgatctccg
     1261 gctactggcg gcgcggccgc accgaggaga ccttccgcca gtggaaacgt gagctcgccg
     1321 aggcggaagc caccgcctga gcgcggtcag aaccagcttg cctgcgcgtc caccgtcgtg
     1381 cgatccagac tggccagcag atcgaactgc ggtccgacct ttggcagctc gaaggtgtag
     1441 aagtactgtg ccgcagcgcg tttgccgtcg tagaaatcgc cctgatggtc gctggcggcg
     1501 atcagctgct cgagccacat ccaggccacc accacatgtc cggccgcctc caggtagaca
     1561 gtcgagttgg cgagggtggt ggcgacatcg cctgccgccc aaagctttcc ggtgatatcg
     1621 accaaccgtt gcactgcctc acgcagctgt tgagcatagg ccgcagtgct gtcggacgcc
     1681 agagccttgt cgatggttgc gttgatcttc tcggcaagca gggtcaggcc agcgccgtcc
     1741 tgcatgacga ccttgcggcc caacaggtcc aggccatgga tgccgtgcgt gccctcgtgg
     1801 atcgagttga gacgattgtc gcggtagcac tgctcgacgt cgaactcctt ggtgtagccg
     1861 tatccgccgt gcacctggat ggccaggctg ttggcctcca cgcaccactg cgacggaaag
     1921 ctcttcgcga tcggagtcag gacttccagc agcagattcg cgcgatcacg ctcggcgccg
     1981 acgctggtgc gcccctcgtc caccagcatc gcgcagtaca ggccgagcgc gagcgcgccc
     2041 tcgacatagg acttctgcgc caacaacatt cgcttgacgt cggcatgctc gatcaacgcc
     2101 acctgcgggc gcccaggatc cttgtcaccg accggacggc cctgagtgcg ctccttggcg
     2161 tactcgacag acgccaggta accggcgtat cccgtcgccg tcgcctgcag tcccaccgcc
     2221 atccgggctt cgttcatcat gtggaacatg taggccaggc ccttgtgctc ctcgccgacg
     2281 agatagccga ccgcgccggg ttcggcatcg ggctgatggg tgccgtcgcc gaagttcagc
     2341 agcgcgttgg tcgtgctgtg gttgcccatc ttgtggttca ggctgaccag cgcgacgtcg
     2401 ttgcgggtgc cgtcggccag aaacttcggg acgatgaaca gcgagatgcc cttcaccccg
     2461 gggccgccgc cgggggcctt ggccagcacg aggtgcacga tgttctcggt cagttcgtgg
     2521 tcgccgccgg tgatccacat cttggttccg gtgatccgat aggtgccgtc ggcggcggga
     2581 acggccttgg tcgtgatgtc ggtcagcgac gagccggcat cgggctccga caggcacatg
     2641 gtgccgaagt agcggccctc gagcatcggt cggacccacg tgtcgatctg ttcctcggtg
     2701 ccgtattcga cgagcaagtt ggcattgccg acggtcagga aggcgtacga ggtggtgctg
     2761 gggttggcgg cccgaaagaa cgccatcgac gcctgcgcca gcaccgtcgg gagctgcatg
     2821 ccgccgactg cctcgtcgaa cgatccggcc acaagtcccg cgccggtgaa cgtgtccagc
     2881 gcgtccttga tctcgtcgat cagcacgact ttcccgtccg gtcccaccgt cggctcgttc
     2941 tggtcgccct tcttattgtg cggcgcaaag catttcgttg cgatgtcgac ggacaaatcg
     3001 aggacggcgt cgaaggtgtc gcgggagtgc tcggcgaagt ggtcgcgctt ggtcagctcg
     3061 actacgtcaa gccactcgta gagcagaaag tcgacgtcac gccgcgagag gaactccgcc
     3121 atgtcggaaa cagcctttca aatgatataa gcgaacgctc attttatgag cgctcgctta
     3181 taatgcgccg atcatcgaca gatgtcaaat acgtgtagga tcaccgaccg tcatgaccat
     3241 cgatcgagct gcggattcag ctgcggcgga acgcatgcgg cgcgccgcca tcgaggcgtt
     3301 cgcggaaacg ggatacggcg gcagctccac gcggcagatc gccaaacggc tcaacatgag
     3361 cgcgacggcc atgtacccgc attaccgctc caaagaagaa ctgctctacg ccatcgcgct
     3421 ggaaggccac agcagcctgc tggccgcgct gaagagggcc gacgacctcg ccgcgagctt
     3481 caccgagcgg ctgcgggcgg tggtggcggc gttcgccagc tggcacgccg aaaaccacaa
     3541 gcaggcccgc gtcgtgcaat acgaactgca cggcttgtcg ccggcccact accgcacgat
     3601 tgtgccgctg cgccaccagg ccaccgacgt catcacggag atcgtcgccg gcggtgtgac
     3661 ggccggcgag ttcgacgtgg acaacgtcga cgagctggtg atggccatct cgtcgctgtg
     3721 cgtcgacgtg tgccgctggt tcccgtcacg ccaacaccgg gacgcacaga agctgggcga
     3781 ggtgtacgcc ggggtggcgc tgcggatggt tcgctgacgg ctgccggggc cgggctggca
     3841 cgctaggcgc atggcattcg gcgattacca gctcgaaatc tatctgcagg gattgtccgg
     3901 catcgtgccg tcgctcccga tgtcctacgc ggaatgggaa gccaaggcgg aagcggcgat
     3961 gccaccgtca atctggtcgt acgtcgccgg tggtgctggc gacgagcgca cccagcgggt
     4021 caatcgcacc gccttcgacg gctggggttt gatgccgcgg atgttcgtcg gcgccaagga
     4081 acgcgatctg tcggtcgagg tgttcggttt gacggtgcca tcgccggtgt tcatggcgcc
     4141 gatcggcgtt accggcctgt gcgcacaaga tggccacggc gatctggcca ccgcacgagc
     4201 cgccgcgcgc accggagtgc cgatggtgat ttcgacgctg accgaagacc caatggaaga
     4261 aatcgccgcc gaattcggtg ataccccagg ctttttccag ctgtacacgc cgaccgaccg
     4321 cgacctggcc gagagtttcg tccggcgtgc cgaggccgcc ggctacaagg gcatcatcgt
     4381 cacgctggac acctgggtac cggggtggcg gccgcgcgac ctgtcgacgt cgaacttccc
     4441 gcaactgcgt ggccgctgcc tggccaacta caccagcgat cccgtattcc gcgcgagcct
     4501 gcagcagccg cctgaggaga acatgcaagc cgcggtgctg cgctgggtct cggtattcgg
     4561 caatccgctg acatgggacg accttccgtg gctgcggtcg ctcaccgatc tgccgctgat
     4621 cgtcaagggc atctgccatc ccgacgacgc gcgccgcgcc agggacggcg gcgtcgacgg
     4681 catctactgc tccaaccacg gtggccgaca ggccaacggt ggcattcccg cgatcgactg
     4741 cctacgagcc gtcgtctcgg ccgccgatgg gctgccggtg ttgttcgatt cgggtatccg
     4801 cagcggcgcc gacatcgtca aggcgctggc gctgggcgcc acggccgtcg gcatcgggcg
     4861 gccgtacgcg tacgggctgg ccatcggcgg tgaggacggc gtcgtgcacg tactgcgctc
     4921 gctgctggcc gaggccgacc tgatcatggc cgtcgacggc tatccgacac tcaaggacct
     4981 gacacccgag gcgctgcggc gcgtcacctg aactgcggcc gggccgtctg cgacggtagg
     5041 ggagtgcaag cgattttcga ggagttcgac gagtaccttg cgctccagcg cggccgctcc
     5101 gagcacaccc gccgcgcgta tctgggcgac ctgcgctcgc tgttcgactt tctcgccgaa
     5161 cgtatgccgg gcgccgggct ggacaggctg agcctgccga ttctgcgctc ctggctgtcg
     5221 gctcaagcgt cggcaggcgc ggcgcgcacc acgctggcgc gacgcacctc ggcggtcaag
     5281 accttcacgg cgtgggcgac gcgccgcgga ttgttgtcga ccgatcccgc cgcgcggcta
     5341 caaatgccga aagcccgacg cacgctgccg tcggtgctga gacaggatca ggctctggat
     5401 gccatggccg cggcgaaatc cggtgctcag caaggggatc cgcttgcgtt gcgcgatctg
     5461 ttggtcgttg agttgttgta cgccaccggt attcgtgtca gcgagctgtg cgggctcgac
     5521 atcgacgacg tcgatcggcc ccggcggctg ctgcgtgtcc tcggcaaggg caacaagcag
     5581 cggacggtgc cattcggaga gccggcgcag gcggcattga cggcgtggct ggacgacggg
     5641 cggccggagc tggccaccgc tgattccggt ccggcgctgt tgctgggcgc ccgcggccgt
     5701 cggctcgacc cgcggcaggc gcgcaccgtc gtgcatcaga ccgtcgccgc ggttgatggc
     5761 gcacccgaca tcggccccca tgggctgcgg cacagcgccg ctacgcatct gctcgagggc
     5821 ggcgcggacc ttcgcgtcgt gcaggaatta ctgggtcatt cgacgctggc taccacgcag
     5881 ctgtacacgc acgtcaccgt caggcgtctg cgtgcggtgc acgaccaggc gcatccacgg
     5941 gcctagtggt gatttgggtg cgccaacgat cgcggagcga ccgtagacac accgaactcg
     6001 cgacggaggg aacgacgcgg gcttcgcggc cgtccaacct gagtgctcga tgagtatctc
     6061 cgtgaacaag acgccgtcat cactctcgcg caagccagac agcgacgggt tggtccgcca
     6121 acgtgacttc cgcgagtcac ggttcagagt tcaattccgg tgtacttggt ttcgagatat
     6181 gcatccaaac cttcgatacc cccctcgcgg ccgataccgg aggccttaat gccgccgaac
     6241 ggtgcgaccg gattgctaac cacaccgcga ttgattccgg tcattccggt gtcgagggcg
     6301 tcggccacct ttaacgctcg cgacagatcc cgggtgaaga cgtacgatgc caatccgtac
     6361 tcggtcgcgt tcgctgcagc gagaacctgc tcctcgctgt cgaactcgta gatcggcgcg
     6421 accggcccaa agatctcctc tgtgcagatg gccgcgtcgt cggggatgtt atggaggact
     6481 gtcggagcgt agaaaaagcc ttcacgctcg acacggtgac caccagtcag gcagtgcgcc
     6541 cccttggcga ccgcatcatg gacgaggtcg ctgaccttgt gcaattggcg ctcgtcgatg
     6601 agaggaccga tggcggtacc agcgtttgat gcatcgccga cggcgacgtt gcgcatgcgc
     6661 tcagtaaagc gttcggtgaa ggtcgcagcg atctctcgct gtacgaagat ccggtttgcc
     6721 gcaacacacg attctcctcc gttgcgcaac ttcgcaacca acgcaccctc gacggcgata
     6781 tcgagatcgg cgtcggcgaa cacgatcaaa gggccattgc cgccaagttc catcgacgtg
     6841 ttcatcacat tctcggctgc ctggcgcagc aggatcttgc cgacgcgtgt cgatccggtg
     6901 aatgagacct tccgcgcgcg gcaatcccgc atgatcgcgg cgacgacctc cgatgaccgg
     6961 gtagtggtaa tgacattgac gacgcctgcc ggtacgccgc agtcttcgag cacttgcgcg
     7021 agcagaaggc tactcagtgg tgtctgctcg gcgggtttga ggatggtagt gcatcccgcc
     7081 gcgagcgcgg gggcaacctt tcgcgctccc atggccagtg ggaaattcca cggcgtgatc
     7141 agcagtgatg gccccacggg cctcttcgac accgcgattc ggtactgccc accgggttca
     7201 atgccgaact gtccgtcgag gtgaagccgt cgggcagatt cagcaaacca ccggaagaaa
     7261 cccgcaccgt agtcgacctc actttgtgct tcagaccacg gcttccccat ttctgcggcg
     7321 atcagcgcag cgaactcgcc acgccgcgtc aggatcgccc ggtaggcgcg gtcgaggatg
     7381 tcggctcgcc gacctgccgg cgtggacgcc caatcgtgct gggattcggc cgcagcagcc
     7441 agagctgcca gaccgtcgac ctcgtcggcg tcggcgactc gcgccagaac atcctcggtc
     7501 gccgggttca ccacgctgat cgagcgagcg cacgcggctg gctgccagcg acccccgatg
     7561 agcagatcgg tcggcacgga cggtagagga gtggtggcgg cggacatcac tgccatggga
     7621 atcgttcctt cctgccgtgg cggtgcaaca gcggcctctc gatccgtgac tggaggggcg
     7681 gatcgtgagg accgcgatcc aatagtgttc aactgtagaa cagatcatgc agatcacaca
     7741 agcgagtagg tgctcgggct ccgcctgtgg gaagggcgcg gtcgcgcgat taaaggcgct
     7801 gacggacgcc ggtcggcagt ttgcggcggc ctcggtctgg cgtctccgag gcaccccgtt
     7861 agtcggtttt cttgcgctgc gcggtgatcc cgcgtgcaag gtcgcgattc tcttcgagaa
     7921 tcgcggtgac gacctggata gctcgggcgg gatcccggga ctcgaaagct tcgaccatct
     7981 cttggtgctc gtgagcgacg ccctcgttgt cgagatactc attgcggtcg taactcagca
     8041 caaggaggaa gtagcgaagc tcgttcagca gtgcggcgta aaactggtcg attcgtgcac
     8101 tcccgagaat gccgacagcg gcctggtgaa tcgcgaggtc acaatcgacg atcgtgcgca
     8161 ggtcatgggt ttcaagcgct tggacatacc gcctgtgcgc atcgcgcatg ggggccagat
     8221 cgctctcggc ggtgagggac tgagctgcga gtcgttcgag gtacagcctg gctttgaaca
     8281 cgtcgacgac atcatcgtcg gtcgggtccc agacctccat gccccggttg aagttgtatc
     8341 tgaccaagcc cgaaccttgg agtaggcgga cagcctcccg gacggtgtta cgtgacaggc
     8401 caagcgatcg ggcgatgccg gcttcgctga tgcgttcacc tgggctaatg gcgccttcga
     8461 tgatcatgct ggtaagaccg tcggctacct gctgggccgt gctcttgccc acgatcgcga
     8521 tgtgatcagc cacagctttc gtcatctatg taactgtact acagctggac aatctcaccg
     8581 gagccggtta gtgtggtgca cgtcacgtag aactgtagaa cagttgaact gttagatagg
     8641 cagagtctac tgttgtacga agtcccgagg agtccccaag tgcaggaaga acaggcagcc
     8701 caggccgatg gttcggcgcc aaagctgatc gtgcatggcg agttgaagag aaactcccta
     8761 cggaaccgcc acctgacgat gattgccctt ggcggcatca tcggcgccag cctcttcgtc
     8821 ggtagcggtt cggtgatcgc tagcgctggg ccggcggcgt tgctgtgtta cgccatcggc
     8881 ggcgtcttgg tagtcctcgt gatgagaatg ctgggggaga tggcggttgc ccgtcccgct
     8941 cttggatcct ttatggagta tgcccgggag gcacttggcg accgagccgg cttcattgtc
     9001 ggttggttgt actggtactt ctgggtggga gtcgtcgcgt tcgaggccgt cgttggcggg
     9061 cgcatcgtca gtggatggac cggggctccg gcctgggtct tcgcccttgg tctgatgttt
     9121 gtgctaaccg taagcaatct gctttcggtc aaatcgttcg gcgagaccga gttctggctc
     9181 gcgtcgatca agatcgcaac catcatcgtc ttccttatcg tcggctcgct atgggtgctg
     9241 ggcgtgttcc cgcattcgca cttctcagtc cccaacatcg cctacggcgg attcttcgcc
     9301 cacggcgcga tgcccgtgct gcaaggtgtg gtaaccgtca ttttcgccta cttcggcacg
     9361 gagatcgtga cgatggctgc cgcggaatcg cgccagccgg cccgcgcgat cgcccgagcg
     9421 acgaacacca tcgtgtggcg aatcatgctc ttctatgtcg ggtcggtcgc gctgctgttg
     9481 atgatcacgc cttggcccga gatcccttcc acatcaagcc cgtttgccgc agccttcgac
     9541 ttgttcgggt ttcccgctgc cgcgaccatt atcaacgtcg tcgtgttgac cgcggccctg
     9601 tcggtgctca actcagggtt atacaccgcc tcgcgcatgg tgtacgcgct gggcggaacc
     9661 gggtgggcgc ctggatgggt gactgacacc aatcgccgcg gcgtgccgtg gaaggcgatt
     9721 ctgctcagca ctgttgtcgg ttacgtcgcg atagtgatga actacgtttc tcctgacacg
     9781 attttcgcgt tcatcatgaa ttctgccggc gccgttgccc tcttcgtgta tctcatcatc
     9841 gcgttttccc agctgcggct gcgttcacaa ctgacgaagc aaggagcctt cgactcgcag
     9901 ctgaagatgt ggtttcaccc ttggctcggc atcttgacca tagtcggaat ctgcgtggtc
     9961 ctcggtgcca tgggttatct ggaaagcacc cgatcgcagt tgttgttgag cctgctcagc
    10021 gtcggtgccc tcctcgtcgc gtacccgttc gcgcgccgct cccgtgagcg tctgctcgcc
    10081 tcagcggtgc cgtcgacgat cgccggctca cagcccaatc cagctcgttc ataaggagtt
    10141 tggctcatga ctgatcagga ccagacctca gggactgcgt tgtggaatgc gcaggcgcac
    10201 atgccatcgg tcgcagaacg ccagatcgtc atttcccgtg gcgaaggcag ctacatctac
    10261 acgaccgacg gccggcgtct attcgacggc accgcgtcgt tgtggttcgc caacatcggc
    10321 cacggccgca aggagatggc ggaagccgct tatcgccaaa tgctcaacct cgagacctac
    10381 catgtgttcg gccgctacac gaccgaccgt tctgtggcgc tggccgaccg gcttgcgtca
    10441 ctctcgccga tcgaggactg caaggtcata tttaccagcg gaggctccga ctcgatcgac
    10501 accgcctgca agcttgcgcg caggcattgg caactccgcg gccgcacctc gaaacgcacc
    10561 attctgtccc gccagttcgc ctatcacgga ctgcacgcct acggaaccag catcaccgga
    10621 ctggacttca accgcgaggg ttacggcacc gagtccttgg tgccagagac ggcccgtgtc
    10681 tccaatgcgg acatcgacgc cgtcgcgcgc gtcatcgacg aaatcggcgc cgaaaatatc
    10741 gccgcattag tattcgaacc catccaaggc accggcggcg tgaatccacc accgcccgga
    10801 tatcttgaag ggctggaacg gatctgccgc gacaatgaca ttctccgcat caccgatgag
    10861 gtgattaccg gttttggtcg caccggtaga atgttcgcgt ccgagcggta cggcctgcag
    10921 tccgacctcg tcaccttcgc gaaaggtgtc acgtccgggt atgcgccact tggcggcatc
    10981 ctcatcgcgc cctcggtgtg ggaaccctac ttttcgccgc agagcgagtt gaccttccga
    11041 cacggcgtca cctattcggg ccatgcgacg gcgtgcgccg tggcagaaac caacctcgac
    11101 attctcgaga aagaacacct cctcgaacgc gcggagaagc tggagctcac gttgcaccac
    11161 gaactcgact cgctcagcga cctggacgca gttgaggaca ttcgagtcgc cggattcctc
    11221 gggggcgtca gcctgcgcgc cgacctcgat gcgccggcga tcgccgatga cctcgtcgac
    11281 cgcggattca tcgtccgcgc gttgcgaggc aacacactcc agctgagccc gccgctcgtt
    11341 acaactgacg aagaagtggt ccagctggcc gccgcgctcc gcgaatccct cgtcgatgcc
    11401 gacaggtgag atctgcgcaa ggaactcggc tccaaaccct cgaagtcctg cgcaacacca
    11461 ctgataaggc gcgagtcttt ctcagtcgcc ggggaccgag cgcggccact tcggccgtgg
    11521 cagttcggtt ttcgacggcg gacgcatgca gtccaggacg gtgtgtaacg gcggccatgt
    11581 cgcgcggccg cgacgcatga ccgcgtaggc cgtcagcact gccttgggtt ctgccagggg
    11641 aagcactttg accccggcgg tggtggcgcg gccgatgggc agcagtccca cgccgtagcc
    11701 ggcgatgatc agatcctcaa ccagttccag gctgtcgatc tcgtgcgcga tgcgtgggac
    11761 gaaagcggcc agcgatgcca gggcccacac ggcagcctca tcagcggtgt tgcgggagtt
    11821 gatgatccac gtttgatctg cgtagtcggc gatctcagca tggccatctg aagtatcggc
    11881 agacacatcc agtccccacg gaaccgacta cagcgcaaac gtttccaaca ccggacccag
    11941 cgacgcgggc gcgaggttgt agtcgtaggt cagcgcgagt tcgaggtcgt cgttggtcaa
    12001 cagcgcgaag gcctcgatgg gttcgaaccc gctgatcacg aactccagct tcggaaaccg
    12061 ctctgcgagc tcggcgatga tcggcagcag tgatactcgg atcccggttg caaagccgcc
    12121 cacgcggacg gtgcctgccg gttcggcatc cgggtagagg tcgaggcgta cgctgtcgac
    12181 ggccgccagg atggtgaaag catgatcggc gaggcgtagg cccgctcacc gaccgctaga
    12241 ggaccgaatc actcacgaag cagggcaatg actttcgcga tgtcgggcat tgccgagtcg
    12301 cgcccaaccc attcgtcgaa cacggtccag tagctcacac cgaagcgctg ctgccgctcg
    12361 atcagcgcct cggccatctg ctcgtgcgtg ccgagcaaga cgaacggcga atcgagcacg
    12421 aactccggcg agatcccgcc gaaggccgcg gcgagttcgg cggccgatgc ttcacggtcg
    12481 tcggtgtgca cgacgaactg aatgagcgcg ttgagttcga tcgcgtcgaa ccggtcaccg
    12541 gcggcgtcac gcacaacggc aatccggtcc tccaggccgg ccgcgtcgaa gtgagtcagc
    12601 tcgaccttcg tggcgtcgcg gttgtggctg aagcccgcca gtccggcgat gtcggcgaca
    12661 cggccggcca actgcaacac ccgcgttccg ttgccgccta caagtaaagg cacggcaacg
    12721 ccgggcgcag cgacgagctc gcccgcctcg gcgcgcacgc agtaatgggc gccgtcgacg
    12781 tcgacgggct cgcccacaag taacgcccgg atgacgtcga ccgcttcgat gagccgatcg
    12841 acccgcgtct tgcccgaatc aaaccgaatg cccgcggcgt cgtactcgga tttcatgtgc
    12901 ccggccccga gccccaattc gaaccgcccg cccgagatcg cggcgacggt cgcggcttcg
    12961 cgtgcggtgt cgatgggatg gcgtaagtcg ttgttcagca ccagcgttcc ggtgtgcagc
    13021 cgatcggtcg ccacagcggc ggccatcgca ccggcaaacg gcgacacaga cgcgaccagg
    13081 tggtcgggaa tcgtcagcac gtcaaaaccg gcgtcctgca ccgagtttgc gaatgcgccg
    13141 aactccgcgc cacgcggcag cgccgtgtgc agaccgaagc gaagcggacg tgaacgatga
    13201 gcgtcggtgg tcatggttcc gacggtaggc gcgtcagcca cccaatggtt tgagccggac
    13261 cggcgtgctg gcgagcagcc ccagcgggtc gacgtagtcc gcgcgcgagg ccggacccca
    13321 catcgccccc caatgcaggc acgccgcggc ggcgcaaccg ccatgtcccg cctccagctc
    13381 gccaatcacc gccccggcgt caatgagctg accaacgcgg accgacgatc gcaccggctc
    13441 atacgtcgtc cgcagcccgc cggaatgcgc gatcgacacc agcgtccgcc cggccagctc
    13501 cccggcgaac accaccgtgc ccgcgccggc cgcataca
//
LOCUS       DASRJA010000282         5509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2865198,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000282 DASRJA010000000
VERSION     DASRJA010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2865198"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..595)
                     /locus_tag="VFC01_12405"
     CDS             complement(110..595)
                     /locus_tag="VFC01_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HZN80473.1"
                     /translation="MNIRKAVTKGLLATAISGALAVVPMALSTGTANADSVNWDAIAQ
                     CESGGNWSTNTGNGTYGGLQFKQATWNANGGVGNPAMAPRAEQIRVAENVLRTQGLKA
                     WPKCGAQAASPAVWANPTMPTAPTMTTGCAAMPTSGLLGFINPRQICTALLNPLGGFG
                     A"
     gene            complement(813..1139)
                     /locus_tag="VFC01_12410"
     CDS             complement(813..1139)
                     /locus_tag="VFC01_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HZN80474.1"
                     /translation="MKNIRKTFGLATIAGALALAPMALATGTANAESGVNWDAVAACE
                     SGGNWSIATGNGYYGGLQFTMGTWRANGGSGSPQHASREEQIRVAENVVRSQGIGAWP
                     VCGRRG"
     gene            complement(2188..2778)
                     /gene="mobA"
                     /locus_tag="VFC01_12415"
     CDS             complement(2188..2778)
                     /gene="mobA"
                     /locus_tag="VFC01_12415"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HZN80475.1"
                     /translation="MTPPVPLAAVILAGGASRRMGRDKANLQYEGTTLLERTVSVVSP
                     RCSPIFVIAAPGQPLPAVEAEVLRDEVRGVGPLLATGRGLRAAADAGLELAFVCAVDM
                     PLLDAELIAVLAGPAVRLGADVVLPWDGRDHYLAGVYRTSLADRVDALVAAGERSMRA
                     LVDRVDTQRIVMAEQPSLANVNTAADLNATLPPRIA"
     gene            complement(2789..3865)
                     /locus_tag="VFC01_12420"
     CDS             complement(2789..3865)
                     /locus_tag="VFC01_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZN80476.1"
                     /translation="MTDLIGSDLGVTPLSKISGVPTTDEPQKAKDFTSDQEVRWCPGC
                     GDYVILNTIRNFLPELGLRRENIAFVSGIGCSSRFPYYLETYGFHSIHGRAPTIATGL
                     ALARPDLSVWVVTGDGDALSIGGNHLIHALRRNVNLTILLFNNRIYGLTKGQYSPTSE
                     VGKITKSTPMGSLDYPFNPVSLALGAEATFVGRALDSDRKGLSEVLRGAAAHRGAALV
                     EIMQDCPIFNDGSFDALRKEGAEERLINISHGEPITFGADGEYCVVKSGYGLEVAKTA
                     DVSADEIVVHNATVEDPAYAFALSRLSEQNLEHMVMGIFRQVNKPTYDDSARSQVVSA
                     RDAKAHDTAALQSLLRGKDTWTVD"
     gene            complement(3862..>5509)
                     /locus_tag="VFC01_12425"
     CDS             complement(3862..>5509)
                     /locus_tag="VFC01_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZN80477.1"
                     /translation="ALKANVSDLPRGGLIIANADEFTKRNLAKVGYEANPLESEELSD
                     YVVQSVPMTTLTLGAVESIGASKKDGQRAKNMFALGLLSWMYGRELEHSEAFIREKFA
                     RKPDIAEANVLALKAGWNYGETTEAFASTYEVSPAKLKSGEYRQISGNTALAYGLITA
                     GQLADVQVVLGSYPITPASDILHELSKHKNFNVLTFQAEDEIAGIGAAIGASYGGALG
                     VTTTSGPGISLKSEAIGLAVMTELPLIVVDVQRGGPSTGLPTKTEQADLLQAMFGRNG
                     ESPVAIVAPRTPSDCFDATIEAARIAITYHTPVMILSDGAIANGSEPWAIPDVSTYAP
                     IEHTFAKSGEPFQPYARDPETLARQFAIPGTPGLEHRIGGLEAANGSGNISYEPKNHD
                     LMVRLRQAKIDGIKVPDLEVDDPTGDAELLMIGWGSSYGPIGEACRRARRKGIKVAHA
                     QLRNLNPFPANLGDVLRRYPKVVLPEMNLGQLALLLRGKFLVDVQSVTKAEGMAFLAD
                     EVEGIIDAALDGTLGDKEIDKAKFARLAAATVETGVGANA"
BASE COUNT          945 a         1761 c         1799 g         1004 t
ORIGIN      
        1 tggaagttcg tccgggtggc cgacgaccgc gtggccccca atcgatggca atggacgact
       61 cggaccgatc ccggcgtgct tgtcgacccg cagggcaacg agttcagctt caggccccaa
      121 aaccgcccag cgggttgagc agggccgtgc aaatctggcg cgggttgatg aagccgagca
      181 gaccgctggt gggcatcgcg gcgcatccgg tggtcatcgt gggcgctgtc ggcatggtgg
      241 ggttggccca caccgccggg ctggcggcct gagcgccgca cttgggccat gccttgaggc
      301 cctgcgtgcg caggacgttc tcggcaacgc ggatctgctc ggcacgcggc gccatcgccg
      361 ggttgcccac gccgccgttg gcgttccacg tcgcctgctt gaactgcagg ccgccgtacg
      421 tgccattgcc ggtgttggtg gaccagttgc cgcccgactc acactgcgcg atcgcgtccc
      481 agttgacgct gtcggcgttg gcggtgcccg ttgaaagggc catcggcacg acggcgagtg
      541 ctccggaaat ggcggtggcc aacaggccct tggtgactgc cttacgaatg ttcatcactg
      601 cggtccttcc ccgaccgttt gaaacaaggc ccgcaagtga ggagcttgtg cgttccccga
      661 tgcggatatc aggtgtttcg ggtgatcaac atcacgtgtt acccaagtgt caaaagtttt
      721 attcgttact tatctggtgt aaggggcctc tgagaaagaa gaggcggcgc caggcaaacg
      781 cccagcaccg cctctttgca gtcgttagtt gactagccgc gcctgccgca gaccggccac
      841 gcgccgatgc cctgcgaacg aaccacgttc tcggcgaccc gaatctgctc ttcacggctg
      901 gcgtgctgcg gcgaaccgct gccgccgttg gcccgccagg tgcccatggt gaactgcagg
      961 ccgccgtagt aaccgttacc tgtagcgatc gaccagttac cgccggactc gcacgccgcg
     1021 acggcatccc agttgacgcc gctttccgca ttcgcggtgc cggtcgccag cgccatcggt
     1081 gccagagcaa gcgcaccggc gatggtggcc agaccaaacg tcttgcggat gttcttcact
     1141 tcgatccttt cgccaagcgc gcgccatcaa cacccgcgcg gagacgcggg cgtggctgcc
     1201 tgtacacagg cgtgggagtt gggccgcacc gtctcggtca ggtgcggcag cgagaggcga
     1261 gacttgcctc gccgggcgtg aggcccgacg gccgacgaag gcgaacttgc cttccaacgg
     1321 ccccactcac acgcaggttc ttggttttga aattatttgc tccgtttgaa gctgttggga
     1381 ccgtacaaac ccggagacgc aaagtcatat caggtggagg gtgtgtcgcg gttagatcac
     1441 gggaagatca cggaccaacc taagaatctc ctgtgcaggt cagccgccgg tttgacggca
     1501 gcggggaatc tgcagttccg gcgtgtcgcc gtgattcaaa tcacgatcat tttgtgagct
     1561 gggacacgcc tacgacgacg gttcaggtgt cgaaatcggc cgtgcgactg ggaaaccacc
     1621 ggcaagggtc gcgaaatggg cgttctctga caacagttcg ctgctcgtcg catcgtctat
     1681 cctgtcgcgt cgggacctta aatccgctgt ccccgttact atttcagcga cacagccgga
     1741 agccggcaac gaccgtcgct gaaccgttga ttgccaaaat gcgtggctgg ctcgcacgaa
     1801 tactattgat ctgcaagatg tttcgatcca gttcgcgctg gtgcgcgtag aatcgcacag
     1861 acagacgata gcggacggcc tgctcaccag cccgcatcgt ggcgatcgag atgcccggcg
     1921 gctgttaaaa cctccgcagg agcgggggta ttgggcgcct tcggcagccc tatactgtgc
     1981 cggaattgcc agctgtattg ttcgggcttc gccaaaactg gtgatatgca actgatttca
     2041 tagccgctgg gcgacctgat ggcgcgctcg acgccgaatg ccaacgccta cgaacgcgca
     2101 tatatcagaa gaataaacgc aagaattgcc gtcgcggaat tacgtgcgta aatgtgtcaa
     2161 attctaaagt cgaaatcagc aaatttgcta ggcaattcgc ggcggcagcg tggcgttgag
     2221 atcggcggcc gtgttgacgt tggcgagcga cggctgctcg gccatgacga tccgctgcgt
     2281 gtcaaccctg tccaccagcg cgcgcatgct gcgctccccc gccgcaacca acgcgtcgac
     2341 ccggtcggcc agcgatgttc ggtacacgcc cgccaggtag tgatcgcgac catcccacgg
     2401 cagcaccaca tcggcaccca accgcaccgc tgggcccgcg agtacggcga tcaactcggc
     2461 atcaagtagc ggcatgtcca ccgcacagac gaatgccagc tcgagtccgg cgtcggcggc
     2521 cgcccgcagc cctcgccccg tcgccagcag cggcccgacg ccacgaacct cgtcgcgcag
     2581 cacttcggcc tcgacagcgg gcagcggctg tcccggtgcg gcgatcacga aaatcggtga
     2641 acatcggggg ctcacgaccg agaccgtgcg ctcgaggagc gtggtgcctt cgtattgaag
     2701 attcgccttg tcgcggccca ttcggcggga tgccccgccg gccaggatga ctgcagcgag
     2761 cggcacaggg ggcgtcacgt cagtgaggtt agtcgacggt ccaggtgtcc ttgccgcgga
     2821 gcagcgactg tagtgcggcc gtgtcgtgtg ccttggcgtc gcgcgcagaa acgacctggc
     2881 tgcgcgccga gtcgtcgtac gtcggcttgt tgacctgacg gaagatgccc atgaccatgt
     2941 gctcgaggtt ctgctcggac agtcgcgaca gcgcgaaggc gtacgccggg tcttccacgg
     3001 tggcgttgtg gacgacgatc tcgtcggccg acacatcggc ggtcttggcg acctccagcc
     3061 catagccgga cttcacgacg cagtactcgc cgtcggcgcc gaacgtgatc ggctcgccgt
     3121 ggctgatgtt tatcagccgc tcctccgcgc cctccttgcg cagcgcgtcg aaggagccgt
     3181 cgttgaagat cgggcagtcc tgcatgatct cgaccagcgc ggcaccacgg tgcgcagcgg
     3241 cgccgcggag aacctcggaa agtccctttc ggtccgagtc cagcgcgcgg ccaacgaacg
     3301 tcgcctcggc gccaagcgcc agcgacaccg ggttgaacgg gtagtccagc gagcccatcg
     3361 gcgtcgactt ggtgatcttg cccacctcag aggtcggcga gtactggccc ttggtcagcc
     3421 cgtagatccg gttgttgaag agcaagatcg tgaggttcac gttgcggcgc aacgcgtgga
     3481 tcagatggtt gccgccgatc gacaacgcgt cgccgtcacc cgtgacgacc cacaccgaca
     3541 ggtcgggccg cgcaagggcc aggcccgtgg cgattgtcgg cgcgcggccg tggatggagt
     3601 ggaacccgta ggtctccagg taatacggaa agcggctcga gcagccgatg ccactgacga
     3661 acgcgatgtt ctcgcggcgc agcccaagct ccggcaggaa gttgcggatg gtgttgagga
     3721 tgacgtagtc accgcacccg gggcaccacc ggacctcctg gtcgctggtg aagtccttgg
     3781 ccttctgcgg ctcgtcggtg gtaggcaccc cgctgatctt cgagagcggg gtgacgccca
     3841 ggtccgatcc gatcaggtct gtcatgcgtt agctcccaca ccagtctcca cggtggccgc
     3901 cgccaaccgc gcgaacttgg ccttgtcaat ttccttgtcg cccaacgtcc catccagcgc
     3961 ggcatcgatg atgccctcga cctcgtcggc caaaaacgcc atgccttccg ccttggtcac
     4021 cgactgaacg tcgaccagga acttgccgcg cagcagcagc gcgagctggc cgaggttcat
     4081 ctccggcagc acgaccttgg ggtagcggcg cagcacgtcg cccaggttgg ccgggaacgg
     4141 gttcaggttg cgcagctgcg catgggcgac cttgatgccc ttgcgccgag cgcgccggca
     4201 tgcctcgccg atcgggccgt agctgctgcc ccaaccgatc atcagcagtt cggcatcgcc
     4261 ggtggggtcg tcgacctcga gatccggcac cttgatgccg tcgatcttgg cctggcgcag
     4321 ccgcaccatc aggtcgtggt tcttcggctc ataggagatg ttgcccgagc cgttggccgc
     4381 ctcgagcccc ccgatgcggt gctccaagcc gggcgtgccc ggaatcgcga actggcgagc
     4441 cagcgtttcc gggtcgcgtg cgtacggctg gaacggctca cccgacttcg cgaacgtgtg
     4501 ctcgatcgga gcgtaggtgc tgacgtcggg aatcgcccac ggctccgagc cgttcgcgat
     4561 cgcgccgtcg gacaggatca tcaccggcgt gtggtaggtg atcgcgatgc gggcggcctc
     4621 gatggtggcg tcgaaacagt cggacggcgt gcggggcgca acgatcgcga ccggcgactc
     4681 accgttgcgg ccgaacatcg cctgcagcaa gtcggcctgc tcggtcttgg tcggcaggcc
     4741 ggtcgacggt ccaccgcgct gcacgtcgac gacgatcagc ggaagctcgg tcatcacggc
     4801 cagcccgatc gcctcggatt tcagcgaaat gccggggcca gaagtggtgg tgacgcccag
     4861 cgcaccgccg tatgaggcgc cgatggcagc gccgatgccg gcgatttcgt cctcggcctg
     4921 gaacgtgagc acgttgaagt tcttgtgctt ggacagctcg tggaggatgt ccgacgccgg
     4981 ggtgatcgga tagctgccga gcaccacctg gacgtcggcg agttggcccg cggtgatcag
     5041 gccgtacgcg agcgccgtgt tgcccgaaat ctggcggtac tcaccggatt tcagcttggc
     5101 aggcgatacc tcgtaggtgc tggcgaacgc ctcggtggtc tcgccgtagt tccagccggc
     5161 cttgagcgcc agcacattgg cctcggcgat atcgggcttg cgggcgaact tctcacggat
     5221 gaacgcctcg ctgtgctcca gctcgcggcc gtacatccac gacagaagcc cgagcgcgaa
     5281 catgttcttg gcgcgctggc cgtccttctt ggaggcgccg atcgattcga ccgcgccgag
     5341 cgtcagcgtc gtcatcggga cggactgcac gacgtagtcg gaaagctcct cgctctccag
     5401 tgggttggcc tcgtagccga ccttcgcgag attgcgtttg gtgaactcgt cggcgttggc
     5461 gatgatcagg ccgccgcggg gcagatcgga aacgttggcc ttcagcgcg
//
LOCUS       DASRJA010000283         5114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2623351,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000283 DASRJA010000000
VERSION     DASRJA010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2623351"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFC01_12430"
     CDS             complement(<1..184)
                     /locus_tag="VFC01_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZN80478.1"
                     /translation="MTDQAEQHGISYASAGVDIEAGDRAVELFKPLAKKATRPEVRGG
                     LGGFAGLFALRGGYREP"
     gene            293..721
                     /locus_tag="VFC01_12435"
     CDS             293..721
                     /locus_tag="VFC01_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN80479.1"
                     /translation="MKTVRWFALLAVATVPASTVLAAPALATPGVNIDAVVISQSTVN
                     GVDYITKEITIQPGGSTGWHYHDGRVFGVVREGTLTRNMADCSEVVSPAGSAVTEDSG
                     PNHVHIGRNLGPVPLVLWVDYIEPAGSPQAEDVPDPGCGP"
     gene            complement(811..1931)
                     /locus_tag="VFC01_12440"
                     /pseudo
     CDS             complement(811..1931)
                     /locus_tag="VFC01_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006553484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1918..2451)
                     /locus_tag="VFC01_12445"
     CDS             complement(1918..2451)
                     /locus_tag="VFC01_12445"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN80480.1"
                     /translation="MATRRVEIGPTGETVRANVARVRNRQGLTLRDVADRLAGIGRPM
                     AHNTVSEIERGARRVDVDDLMALAVALGVSPVTLLMPAVHSVEPQDDVESTGVAVPVP
                     AERLWEWLTAEYPLFDMEFFEFAGRAWPGWVRERWTERLNAARKKGLDGLYARVPPAA
                     RPGWMSGSGEEVSSGDD"
     gene            2550..2753
                     /locus_tag="VFC01_12450"
     CDS             2550..2753
                     /locus_tag="VFC01_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HZN80481.1"
                     /translation="METNLIPASEMKTIFPGTTDQYWAALRHKGTGPNYVKLLRKVYY
                     DRVDIEAWIEGNRFTRTDRPVNA"
     gene            3246..4481
                     /locus_tag="VFC01_12455"
     CDS             3246..4481
                     /locus_tag="VFC01_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80482.1"
                     /translation="MSDNLFERPVLAHIRDFARSRRVCPESTLAAVLVRANCMIPPHV
                     TVPPIVGGRTSLNLFAALVGPSGYGKGGSESAGRDAIEFVGATVPQPLLELNPGSGEG
                     LAKTFQVTGDENVVHTALFTAPEVDTLSALMGRQGSTLEGELRKVFAGESLGFTNAQK
                     HTRTHVPRLSYRAGLVVGVQPRRAGPLLNGADGGTPQRFVWASVLDRDMPEVAPECPG
                     VLEVKVPPFPSRPDGGFVDLYVPDEPREAIDSHQLAMHRGDIEVDPLAGHALLTRLKV
                     AAALMLLDERARMDEEDWRLAGRIMLRSDATRVAVQREMAAKARGDNRARAWAADERD
                     EFASDRKLIRCKQGIIRWLDRLRDGEHLARRDLSPKLKAELRPYLSACLKPWPFLRSP
                     RQNPPSLPRHGCSGCCQSF"
     gene            4631..5086
                     /locus_tag="VFC01_12460"
     CDS             4631..5086
                     /locus_tag="VFC01_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80483.1"
                     /translation="MITAAVPREYITIVGVSIALVIALIVPTWLIRDWALQKADEALT
                     SDVVNGPVVKPFSLSDVIPVLDVSTEMVNVEWICGADKRRPNVFDGKDTSSAILLGET
                     SSSLFVRMDPPGTTPIVKLPAECAVATRNLPKPKPTTSSGIDVAGDSGP"
BASE COUNT          943 a         1745 c         1521 g          905 t
ORIGIN      
        1 gaggttcgcg gtaaccgccg cgcagcgcga acagcccggc gaatccaccg agaccgccgc
       61 gaacttcggg cctggtggcc ttcttggcca gcggcttgaa cagttcgacg gcgcggtcac
      121 cggcttcgat gtccaccccg gccgatgcgt aggagatgcc gtgttgttcg gcttgatccg
      181 tcatcgagag caaggctacc gtcgttgccc gcgcctcggt aaccggtcga tcctgagcac
      241 ttccccttga tcgcctcgca cgatcgcgcg cggtgtgaga acgtgaccga ccatgaaaac
      301 agttcgctgg tttgcgcttc ttgccgtggc gaccgtgccg gcttcgacgg tcctggccgc
      361 gcccgcgctg gccacgccgg gggtgaacat cgacgcggtc gtcatctcgc aatcgaccgt
      421 caacggagtc gactacatca ccaaggagat caccatccaa cccgggggca gcaccggctg
      481 gcactatcac gacggccgcg tgttcggcgt cgtccgggag ggcacgctca cccgcaacat
      541 ggcggactgc tcggaggtcg tctcccccgc tggctcggcc gtcaccgagg acagcggacc
      601 gaatcacgtc cacatcggcc gcaatctcgg gcctgtccct ctggtgctgt gggtcgacta
      661 catcgagcct gccggcagcc cgcaggctga agacgtgcct gaccccggct gcgggcccta
      721 gggcctgcgg agcgccgaga cgttgtcgtt ctcggccaca ccacaaacct ccctcctatt
      781 gccacaggta gacaccagtt tttgtgactg ctagccaccg tctgcactgc cccttgggca
      841 cattttgggc acaccctcac gcgagtagcg gctgtgcaac gtgaccgcca cagcgtccag
      901 gtcgtcgtcg aacaagtccg cgtaggtgtc cagcgtcatc ttggcgctgg cgtgcccgag
      961 catccgctgt agggccagca cgttgacgcc agcactgatc gccagcgacg cgcaggtgtg
     1021 ccgcagatcg tgaggcgtga tcttctggac tctggcctta gtgaccgccg cggcgaacca
     1081 cccaccgaca gacttcgggc gcggcagata cccgccagcc ggccccggga acaccagttc
     1141 acccggagcc ttgccccgac acttcaccga cagctcgtcg agcacgaacg tcgccaccgg
     1201 caccgaacgc gccttgcggc ccttcgtcgg tccgactgca tggcgggcgc ctacctcgac
     1261 cgcgctgacc gacaccgaca gccgacgccg caggaactcg acatcggcga cccgcagggc
     1321 gatcgcctcc gaccacctca agccgcagta ggccagcacg aggacgagcg cacggtgctc
     1381 gtccgactgc tcggccagcc gcgccagtca tcggcggaca ggtatacgtg cctcttggcc
     1441 gtttggtgcg gcaggttctc cacgccacgc gccgggttga ctgccagccg cttggccttc
     1501 accgcgtcgg acagaatccc cgacagcacg acgtgcgccc ggcgcaccgt ggtgacacca
     1561 ctaccggctc ggaccatggc tgcaatccat gattcgacgc cgagcacgtc cacgtcggcc
     1621 acgctcatcg ctccccagcg cggcatgaca tggacgcggt acgcggactc aaacatccgc
     1681 cagctcgacg gcgctgtggc ctgttgcttg cgcgccaacc agtcggcggc cagctcgtca
     1741 accgtcgcac ggccgagcgc cggcgcgacg tactcgcctt tgagcttcga cacttcgacc
     1801 gtggcggcga actcctttgc gtcccttacg gttcggaacc cgcgccgttt cgtctgcttc
     1861 cgatccggcg tccgatacct aacctggtat cgcttcccgt ctgcggtttg gtacgcctca
     1921 atcgtcgcca ctcgacacct cttcgccgga tcccgacatc caccccggcc tggcggccgg
     1981 tggcacgcgc gcgtacaacc catctagccc cttcttacgc gcagcgttca acctttcggt
     2041 ccatctttcg cggacccagc ccggccacgc ccgtcctgcg aactcgaaga actccatgtc
     2101 gaacagcgga tactcggcgg tcagccactc ccaaagacgc tccgccggaa caggaacggc
     2161 cacaccggtc gactcaacat catcctgggg ttcgacactg tgaaccgccg gcatcaacaa
     2221 cgtgaccggc gaaacgccaa gcgccacagc caacgccatc aggtcatcca catcaacccg
     2281 gcgagcacct cgctcaatct cactgaccgt gttgtgcgcc atcgggcggc cgatgccggc
     2341 gagccggtcc gctacgtctc gcaacgtcaa gccctgccgg tttcggaccc gcgccacgtt
     2401 cgctcgcaca gtctcccccg tggggcctat ctccactcgc cttgtcgcca tcatgcagat
     2461 cgtaggacga aacttgcaga atgccaagtg ctcgggtatt aatgaggata acccgaacaa
     2521 gtgtcagcat aatctgacca gcagacacaa tggagaccaa cctcatcccg gcgtcggaaa
     2581 tgaagaccat cttccccggc accaccgacc agtactgggc tgcgctgcgg cacaagggaa
     2641 ccggaccgaa ttacgtgaag cttttacgca aggtgtacta cgaccgcgtc gatatcgaag
     2701 cctggatcga gggcaatcgc ttcacccgga ccgaccgtcc agtgaacgca tgaacatcga
     2761 cagcgatcag gggccgcctg aaaaagaaga ccgggccccg caccaggacc cggcttccaa
     2821 cgttgaggtc accaccacca acggcataaa gaagtctacc gtccgctgcc gacgctgttc
     2881 gcgaccgcta cgggcagttg tgtcgcaagc gcgatcctgc ggaccagtgt gttggcgcaa
     2941 gcatcgcact gaggcgattg cagcatgagc gaccaactac gccagcgccg ccgcggctac
     3001 gccgcttcgc gacgaatgac accgctaggc ccgtgtggct gcatcaggca ccccgaggtc
     3061 gataggcacc gatgcgacgc gcagatcagc gacgttcagg ctgaggctgc gacggctgcc
     3121 attcagcacc tgaacagcat cggcgcaccg ggcttgctcg atggcgacac ctgccgggcc
     3181 atatggcgca tcggccaccg agatctggcg gtccgctgct acgggtacgc aacggggggc
     3241 gcggcatgag cgacaacctc tttgagcgtc ctgtgctcgc ccacattcgc gacttcgccc
     3301 ggtcgcggcg tgtatgccca gagtccacct tggccgcggt cctggttcgt gcgaactgca
     3361 tgatcccccc gcacgtaacg gtgcctccca tcgttggcgg ccggacgtcg ctaaatctgt
     3421 tcgccgcctt ggtcgggccg tctggctacg gcaagggcgg ctcggaatca gcaggccgag
     3481 acgccatcga gttcgtcggc gccacggtgc cccagccgtt gcttgaactg aatccgggaa
     3541 gcggggaggg actcgccaaa acgttccagg tcaccgggga tgagaacgta gttcacacag
     3601 cattattcac tgcacccgag gtcgacacgc tttcggcgtt gatggggcgg caaggctcga
     3661 cacttgaggg tgagctgcgc aaggttttcg cgggcgaatc cctcggattc accaacgcac
     3721 agaagcacac gcgaacccac gtgccgcgac tgtcgtatcg tgccggcctc gtcgtcgggg
     3781 tccagccgcg ccgggccgga ccgctgttga acggcgcaga cggcggcacg ccgcagcgtt
     3841 tcgtatgggc atcggtcctc gatagagata tgcctgaggt ggcccccgaa tgtccgggcg
     3901 tgctcgaggt gaaggtgccg ccgtttccgt cgcgccccga cggcgggttt gtggacttgt
     3961 acgttcccga tgaaccgcga gaggcgatcg attcacatca gttggcaatg catcgaggcg
     4021 atatcgaggt cgatccgcta gccggtcatg cgcttcttac tcggctcaag gtcgctgcgg
     4081 cgctgatgct cttggacgaa cgcgcgcgaa tggacgaaga ggactggcgc ctagcgggtc
     4141 gcatcatgct tcggtcagac gcaacccgtg ttgctgtgca acgtgagatg gcggcgaaag
     4201 cccgcggcga caaccgtgcc cgcgcttggg ccgccgatga acgcgacgaa ttcgcctccg
     4261 accgcaagct cattcgatgc aaacaaggca tcatccgatg gctcgacagg ctccgcgatg
     4321 gcgagcacct tgcacgacgg gacctgagtc cgaaactcaa agccgaactg cgcccgtact
     4381 tgtcggcgtg cttgaaacca tggccctttc tccggagtcc aagacagaac ccgccgagcc
     4441 tgccaagaca tggctgcagc ggttgttgtc aatccttctg aaaccgtggc ggcggtggcc
     4501 cgcactacgc gcaaccgtgt tgggcggact acttgcaggc ttgttgcttt ggtaccttta
     4561 cgtccttcgc ccgccgaact tgccactgtc gcacatgttt atggcttggg ttgcgatgtt
     4621 gattggggcc ttgatcaccg ctgccgtccc gcgggaatac ataactattg tcggcgtgtc
     4681 gattgcccta gtgatcgcgc tcatcgtgcc aacgtggctc attcgcgatt gggcgctcca
     4741 gaaggcagat gaggcactga cgagcgacgt ggtcaacggc cctgtggtca aacccttttc
     4801 gttatccgat gttatccccg tcttagacgt ctcaaccgag atggtgaatg tcgaatggat
     4861 ctgcggtgcc gacaaacgcc gcccgaacgt cttcgatgga aaggacacaa gcagcgccat
     4921 actccttggt gaaacttctt ccagcttatt tgttcgcatg gacccccctg gcacaacgcc
     4981 gatcgttaag ctcccagcgg aatgcgccgt tgcaacacgc aacctgccga agccgaagcc
     5041 cacgacgtcc tcaggcatag acgtggcggg tgattccgga ccttgatgcc ccacgcggca
     5101 agtggatccc acga
//
LOCUS       DASRJA010000284        17875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3426119,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000284 DASRJA010000000
VERSION     DASRJA010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17875
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3426119"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFC01_12465"
     CDS             <1..275
                     /locus_tag="VFC01_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZN80484.1"
                     /translation="VVDRQIRAVTPNPGAWTMIGDLRVKIGPVTVEDAPDSLAPGVIR
                     VERKGVHIGTGSQPVLLGQIQAPGKKPMNAADWARGARLDESPQAQ"
     gene            272..1642
                     /locus_tag="VFC01_12470"
     CDS             272..1642
                     /locus_tag="VFC01_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZN80485.1"
                     /translation="MTKPRNKRPPRRKPLDPARRAAFDVLRAVSERDAYANLALPALL
                     RERGITGRDAAFATELTYGTCRTRGLLDAIIAAAAGRPPEKIDPVLLDLLRLGTYQLL
                     RTRVEQHAAVSTTVEQAGIEFDSARAGFVNGVLRTIAGRDEQSWVDELAPPADSDPVG
                     HTAFAHAHPRWVAQAFADALGADAAELDALLASDDDRPSVHLASRPGVLTADELATQV
                     DGTVGRYSPYAVYLPGGDPGRLESVRDGAALVQDEGSQLVARALTLAELDGPDAGRWL
                     DLCSGPGGKTALLAALGDSAHVTAIEPAARRAEMVEQNTRGLPVEVLRVDGRDPGLEP
                     GFDRVLVDAPCTGLGALRRRPEARWRRQPGDVPQLAKLQKELLGSAIKLTRPGGVVLY
                     ATCSPHLAETVGIVADALRRHPVTALDTRPLFDPVDDLGDGPYVQLWPHRHGTDAMFA
                     AALQVG"
     gene            1648..2325
                     /gene="rpe"
                     /locus_tag="VFC01_12475"
     CDS             1648..2325
                     /gene="rpe"
                     /locus_tag="VFC01_12475"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZN80486.1"
                     /translation="MAEPLIAPSILAADFAELGDEVAAVTGADWLHVDVMDNHFVPNL
                     TIGLPVVEALLKRTDIPMDCHLMIDNPDKWAPPYAEAGAYNVTFHAEATDNPVGVARD
                     IRAAGAKAGLSVKPGTPIEPYLEILPEFDTMLIMSVEPGFGGQKFIPEVLAKVGIVRR
                     LVDSGELTIVVEIDGGINEDTIEEAALAGVDCFVAGSAVYSAEDPAAAVESLRRQAAS
                     ASKHLRL"
     gene            2322..3323
                     /gene="ribD"
                     /locus_tag="VFC01_12480"
     CDS             2322..3323
                     /gene="ribD"
                     /locus_tag="VFC01_12480"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZN80487.1"
                     /translation="MNLDAAMRLAVEQADRVKGSTYPNPPVGAVILDRGGEIAGVGAT
                     EPPGGPHAEVVALRRAGQRAIGGTAVVTLEPCNHYGRTPPCVDALVTSKVSRVAYAVA
                     DPNPEAAGGAARLAESGIEVEAGVLTDAVAGGPLREWLHKQRTGLPHVTWKFATSVDG
                     RSAAADGTSQWITSEAARADVHRRRAGADAIIVGTGTVFVDDPVLTARLPDGALADRQ
                     PLRVVVGEREISSEARVLNDDSRTMVIRTRDPHEVIKALSDRTDVLLEGGPTLAGAFL
                     RAGVIDRILAYVAPILLGGPITAVDDVGVLSIAHAQRWRFDGIEPIGPDVLLSLVSA"
     gene            complement(3304..3735)
                     /locus_tag="VFC01_12485"
     CDS             complement(3304..3735)
                     /locus_tag="VFC01_12485"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZN80488.1"
                     /translation="MHTSTQLVAELIDAMNAGHLDKIRDLVTDDFVDHGSPVPLPPGP
                     DGYIAILTFVTTVLQIRYEVHDVIADGDMVAIRATAHGVNASSPQGIEPTGRSFAMKT
                     AHFFRSRDGRLCEHWGVRDELDVLYQVGALTPPAMVMPTPD"
     gene            complement(3767..4780)
                     /locus_tag="VFC01_12490"
     CDS             complement(3767..4780)
                     /locus_tag="VFC01_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN80489.1"
                     /translation="MGDLSIQNLTVEYASGADTVRPIDGLNLDVAAGSLVILMGPSGC
                     GKTTLLSCLGGILKPSDGAIKFGDVDVTALDARGLSTYRRETVGIVFQAFNLVPSLTA
                     MENVMVPLSAAGVSRRAAHKRAGQLLARVGLEDRMSHRPGDLSGGQQQRVAVARAIAL
                     DPPLVLADEPTAHLDFIQVEEVLRLIRELASGDRMVVVATHDSRILPLADRVVELVPD
                     FASTDRPPDTVRLEAGEVLFEQGTMGDLIYVVSEGELEILRELASGGEELLKVVTSGD
                     YFGEIGPVFHMPRNATVRARTDATVVGYTVRAFRERLGLGGVRDLIEHRPLEMNDEGS
                     ESD"
     gene            complement(4784..5833)
                     /locus_tag="VFC01_12495"
     CDS             complement(4784..5833)
                     /locus_tag="VFC01_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN80490.1"
                     /translation="MLIAALRDMQWRKRRFVIAILSTSIIFAMTLALTGLANGFRVEA
                     NKTVDSLGVDLFLVKAGASGPFVGATPFAPTDLRRIAASPGVIAAVPLAYAGGTATLG
                     DTTRNVDIFGAPERGPGMPAVSEGRPPSTPDEVAVSSTLGRRIGDDVEIASRRLRIVG
                     IVENSTALANLPNVFLTTEGAQRLVYGGQPLVASVGIRGTLGQVPDGYRGIDRAGAIA
                     DLLRPLKVAVNSITIVAVLLWVVAALVVGSVIYLSALERLRDFAVFKAIGVPTRNIAA
                     GLALQSVIVALLAALVGAGLSRLLGPLFPMQVIVPAEAFVALPVIAVVIGLIASATGL
                     RRAVSVDPALAFGGP"
     gene            complement(5879..6442)
                     /locus_tag="VFC01_12500"
     CDS             complement(5879..6442)
                     /locus_tag="VFC01_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZN80491.1"
                     /translation="MATSLNGKTIAFLVAPEGAEQVELTEPWKAIEQAGGTPELVSTE
                     VGKVQAFNHLTPADTFEADKAADRVSSSDYAALVLPGGVANPDFLRTNDAAVAFVKSF
                     FDAGKPVAVICHGPWTLIEADVVRGRTMTSWPSVATDLRNAGANWVDDEVVECSRGPN
                     TLVSSRKPDDLPAFCNTLVGAFAKDKS"
     gene            6593..6745
                     /locus_tag="VFC01_12505"
     CDS             6593..6745
                     /locus_tag="VFC01_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80492.1"
                     /translation="MTALQDWLSTRSVDPKGVKALICDALRSLTAEEPAKVAEPTLLR
                     RGIERC"
     gene            complement(6747..8435)
                     /locus_tag="VFC01_12510"
     CDS             complement(6747..8435)
                     /locus_tag="VFC01_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN80493.1"
                     /translation="MQASEATRQAATSRRVAISAGSLAVVLGALDTYVVVTIMTDIMK
                     DVGIGINQIQQVTPIITGYLLGYIAAMPLLGRASDRFGRKLLLQVSLAGFAVGSVITA
                     LSTDLTMLVIGRVVQGTASGALLPVTLALAADLWAARTRAAVLGGVGAAQELGSVLGP
                     MYGILLVWLFNHWQAVFWVNVPLAVIAMVMVHFSLPSKAQTDHPEKVDVVGGVLLAVA
                     LGLTVVGLYNPAPDGKQVLPSWGLPVLIGALVAAVAFFVWEKVAKTRLLEPAGVHFRP
                     FLAALGASLCAGAALMVTLVNVELFGQGVLGQDQDQAAFLLLRFLIALPIGALIGGWL
                     ATKVGDRIVAFAGMLITAGGYWLISKWPVDLLAAKHHLGIISLPMLDTDLAIAGLGLG
                     LVIGPLTSATLRVVPAAQHGIASAGVVVARMIGMLVGIAALSAWGLYRFNQYLQERLA
                     ALPAPTADTPGGILIAQGARLKTATIEAYTMQYGEIFGITAIVCVIGALLGLLISGRH
                     EHADEPAPPVSPVDADDTPTTLIDAPTQAISTRPDPPDQTVRIPQQPPPGRHRR"
     gene            complement(8435..9133)
                     /locus_tag="VFC01_12515"
     CDS             complement(8435..9133)
                     /locus_tag="VFC01_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HZN80494.1"
                     /translation="MQTRLLAIFAALFAAIALIAGCSKSQDSSKELPDAATLLKESAE
                     TTKSQSSVHLKLAVQGEIKELPIETLEGDLTNAPAVAAQGKANLIFLGQKLADVGFVV
                     ADGNLFAALTSGGAYQDFGPAADIYDVSAILNPDTGLANVLANFTDPKADGRETINGV
                     ETVRVTGNVTADAVNKIAPQIAATGPVPATAWIQEDGNHELAQAKLDVSPDNSITMTT
                     TEWGKQITVTKPAV"
     gene            9276..9881
                     /locus_tag="VFC01_12520"
     CDS             9276..9881
                     /locus_tag="VFC01_12520"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZN80495.1"
                     /translation="MFTGIVEELGEIVSKEELGDSARLVIRGPVVTSDAGHGDSIAVN
                     GVCLTVVEVLPDGSFSADVMGETLNRSSLRTVGVGSHVNLERAAAVNSRLGGHIVQGH
                     VDGTGHVIARTPSEHWMVVRIALPTALSRYVVEKGSITVDGVSLTVSGLGHDWFEVSL
                     IPTTLDLTTLGRAEVGTRVNLEVDIIAKYVERLMSPDNSST"
     gene            10113..10505
                     /locus_tag="VFC01_12525"
     CDS             10113..10505
                     /locus_tag="VFC01_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80496.1"
                     /translation="MNLKKIAAGAAMVGVLGFSAVGFGGGAQAKPHVPGPCVPGIDCW
                     IPGVDPPGHNPFGPPGQVMQGNPVVPGLTGVPPGHWGDPTLFGLPPTWLPGNWIDLGI
                     PGPLDVVWNPDLAAWGIWWAGQFIPYVA"
     gene            10822..12093
                     /locus_tag="VFC01_12530"
     CDS             10822..12093
                     /locus_tag="VFC01_12530"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HZN80497.1"
                     /translation="MTRLDSVERAVADIAAGKAVVVIDDEDRENEGDLIFAAEKATPE
                     LVAFMVRYTSGYLCVPLDGAICDRLGLLPMYAVNQDKHGTAYTVTVDAKNGVGTGISA
                     SDRATTMRLLADPSSVADEFTKPGHVVPLRAKDGGVLRRPGHTEAAVDLARLAGLQPA
                     GAICEIVSQKDEGAMAQTDELRVFADDHNLALISIADLIEWRRKHEKHIERVAEARIP
                     TRHGEFRAVGYTSIYDDVEHVALVRGDIAGPHGDGHDVLVRVHSECLTGDVFGSRRCD
                     CGPQLDAAMAMVAREGRGVVLYMRGHEGRGIGLMHKLQAYQLQDAGDDTVDANLKLGL
                     PADARDYGIGAQILVDLGVRSMRLLTNNPAKRVGLDGYGLHIIERVPLPVRANAENIR
                     YLMTKRDRMGHDLIGLDDYDEAVPGEFGGAL"
     gene            12116..12592
                     /gene="ribH"
                     /locus_tag="VFC01_12535"
     CDS             12116..12592
                     /gene="ribH"
                     /locus_tag="VFC01_12535"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZN80498.1"
                     /translation="MSGGAGVPDLPDLDASGLTLGIVASTWHEKVCDALLEGARKVAA
                     DAGIHNPTIVRVLGAIEIPVVAQALAANHDAVVALGVVIRGETPHFDYVCDAVTQGLT
                     RVSLDASTPVANGVLTTNTEQQALDRAGLPDSAEDKGAQAAAAALSTALTLRDLRS"
     gene            12589..13041
                     /locus_tag="VFC01_12540"
     CDS             12589..13041
                     /locus_tag="VFC01_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZN80499.1"
                     /translation="MSDWDIEIRPHLTPYFAYAAAAVILAVHIAVGALLKISSTGVVF
                     QTADQVAIGLLGGVIAGAVLLFTRPRLRVGSAGVAVRNLFGYKLIPWSDVVGVSFPRG
                     ARWARVDLPDDEYIPVMAIQAVDKERAIDAMDRVRGLLAHYRPDINSH"
     gene            complement(13028..13504)
                     /locus_tag="VFC01_12545"
     CDS             complement(13028..13504)
                     /locus_tag="VFC01_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN80500.1"
                     /translation="MTAATARVARLTEADWRVFAGVRLRALTDSLGENDPQYQHEVAF
                     TAAQWRRRLRDHAQFAVLIDNRPVGLIGAQLESAETVYLYSLWLDPTARRRGLARPLV
                     AAAVDWARDRRARTVTLRVAADNTVARGVYEALGFTLNATETPSARGEVAMTLSVS"
     gene            complement(13501..15465)
                     /locus_tag="VFC01_12550"
     CDS             complement(13501..15465)
                     /locus_tag="VFC01_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HZN80501.1"
                     /translation="MRIPLLRKVVAPLAGVVLVLAGCAAEENAPPTGTTVAGPCSVIP
                     NGTPAPKTSAAPATASVTTSKNISTNPEVATGYRKDMTAVKTAHYAVATANPLATQAA
                     CQVLNKGGTAADALVTAQAVLGLVEPQSSGIGGGGFLLYYDAAAGTVQAYDGREVAPN
                     AATENYLRWISDADRTEPKPDARASGRSIGVPGILRLLQDVHTEHGKTAWRDLFTPAV
                     AMADDGFEISPRLAAAIADAAPKLKVDPNASAYFLNSDGSAKTSGTRLTNPGYSKTLG
                     AIASDPQAFYTGDIARDIIAAASDTAGGRTPSLMTLEDLSGYAAKKREPLCTPYRGKE
                     ICGMPPPSSGGIAVAATLVMLEHFAMADHKPTDVDLNGGKPAVMGVHLISEAERLAYA
                     DRDKYVADTDFVPLPGGSPNTLLGSDYLAGRAALISEQHTMGTAKPGEFGPPTTPAPP
                     TPEHGTSQISIVDAQGNAAAMTTTVESAFGSFHMVDGFILNNQLTDFSAEPVKDGVPV
                     ANRVQPGKRPRSTMAPTLVFDRGAQPGAKGPLYAVLGSPGGSVIIQFVVKTVVGMVDW
                     GLDPQQAASAVDFGAANTPKTNVGGEHPNIDISDNGDHDPLVQGLRKLGHQVDLADQS
                     SGLSAIARDVAGWVGGADPRREGLVMGDTR"
     gene            complement(15521..16573)
                     /locus_tag="VFC01_12555"
     CDS             complement(15521..16573)
                     /locus_tag="VFC01_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZN80502.1"
                     /translation="MRGGGLDETDLHRAVELEGRQPDIPAAFRPRLQNAILRGWAGDL
                     TLAHEEMQSIRRHYIERGEDNEVIYAAFHSVLLEVWRADFTEASRVAEDAAEGLVASR
                     RCGSSLLASWPAITLGFLDVSLGNYGSALHVLDPLLAALDTVGTEIFTAAFIPDAVEA
                     MIQVGRIADAEPLVAALERNGHRLDRPWMLAVGARCRAMLLAAQGDIEGASAAAATAI
                     SEHARLSMPFERARTQLVAGQLYRRQRQRKAAAEILQEALAAFEEMGVPLWADRTRDE
                     LARAHAARIGGVDLTPSEQRVAELAASGMTNRGVAAALFLSPKTVEANLARIYRKLGI
                     RSRAELGQRMSRAEEA"
     gene            complement(16682..17347)
                     /locus_tag="VFC01_12560"
     CDS             complement(16682..17347)
                     /locus_tag="VFC01_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80503.1"
                     /translation="MATVCVAAPTVEVVGRAIDADSHRAAELLEKAEDNGIVTLVGHR
                     VRFVHPLLAHGMYTNVPAAQRRRMHRRLAAIFDEPELRARHLALATTSGDRATLQSLD
                     AGAEAARLRGAPAAAVELLDLAFGLGGDTPERRIRLALYHFSAGHPGRARALLEVTIR
                     DLSPDHLRADAVSLLAVMGLLNGIFPETASLLKQALNESGEDHSHRAQVLIALSFARL
                     NFG"
     gene            complement(17292..>17875)
                     /locus_tag="VFC01_12565"
     CDS             complement(17292..>17875)
                     /locus_tag="VFC01_12565"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN80504.1"
                     /translation="ALVVEGEVGIGKTTLWLAAIDQARERGFRVLSARTAQTESVRAY
                     TSAADLLADVDEIFWGDLPSPQREALDRMLLRSDGRSTNQRTLAAGFLSLVNRIAAES
                     PTLLAIDDLQWLDTSSRDVLAYVARRLSTRVGVLGTLRTDKTSSTGASWLQMPRPDAI
                     TRLGLQHRSCGTGRALGHSVCRRPDGGGRRPGD"
BASE COUNT         2982 a         5827 c         6050 g         3016 t
ORIGIN      
        1 atgtcgtcga tcggcaaatc cgggccgtca caccgaatcc aggcgcatgg acgatgatcg
       61 gcgacctgcg cgtcaagatc ggcccggtca ctgtcgaaga cgcgccagac agcttggcgc
      121 ccggcgtgat tcgcgtggag cgcaagggcg tccacatcgg cacgggttcg cagccggtgc
      181 tactcggcca gatccaggcg cccggcaaga agccgatgaa cgccgccgac tgggcacgtg
      241 gcgcccgcct cgacgaatcg ccgcaggccc aatgaccaag ccgcgcaaca aacgcccgcc
      301 gcgacgcaaa ccgctggacc cagcccgtcg ggcggcgttc gacgtgttgc gtgcggtttc
      361 agagcgcgac gcgtatgcca acctcgccct gcccgcactc ttgcgcgaac gcggaatcac
      421 cggccgcgac gcggcgttcg ccaccgaact cacctacggc acctgccgca cccgcggcct
      481 gctcgacgcg atcatcgccg cggccgcggg taggccacct gagaagatcg accccgtatt
      541 gctggatctg ttgcggctgg gcacctatca actgctgcgc acgcgggtgg aacagcacgc
      601 cgcggtgtcc accaccgtcg agcaggcagg catcgaattc gattccgcac gagccggttt
      661 cgtcaacggc gtgctgcgaa ccatcgccgg gcgcgacgag cagtcctggg tggacgaact
      721 cgccccgccc gcggatagcg acccggtcgg acacacggcc ttcgcgcatg cccatccgcg
      781 ctgggtcgcg caggccttcg ccgatgcact cggcgctgat gcggcggagc tggacgccct
      841 gttggccagc gacgacgacc gcccgtcggt gcatctcgca tcgcggcccg gggtgctgac
      901 cgccgacgag ctggccacac aggtcgacgg cacggtgggg cgctactcgc catacgctgt
      961 ctacctgccc ggtggcgacc ccggtcggct cgagtcggta cgcgacggcg ccgcgctggt
     1021 gcaggacgag ggcagtcagc tggtggcgcg ggcattgacc ctggccgagc tggacggccc
     1081 cgatgcgggc cgttggctcg acctgtgctc gggaccgggc ggcaagacag cattgttggc
     1141 cgcgttgggc gacagcgcac acgtcaccgc gatcgaaccc gccgctaggc gggccgaaat
     1201 ggtcgagcag aacacgcgcg gccttccggt cgaggtgcta cgcgtcgacg gccgcgaccc
     1261 cggactggaa ccgggattcg accgggtgct ggtcgacgcg ccatgtaccg ggcttggcgc
     1321 gttgcggcgc aggccggagg cccgttggcg gcgccaaccc ggcgatgtcc cgcaactcgc
     1381 caaactgcaa aaggagctgc tggggtcggc gatcaagctg acccgacccg gcggagtggt
     1441 gctctacgcg acgtgttcgc cgcatctggc cgagaccgtc ggcatcgtcg ccgacgcgct
     1501 gcgtcgccac ccggtcacgg cgctggacac ccgaccgctg ttcgacccgg tcgacgatct
     1561 cggtgacggg ccctacgtgc agctctggcc gcaccggcac ggcaccgatg cgatgttcgc
     1621 tgcggcgctc caagtagggt gagcgccatg gcagaaccgc tgatcgcgcc gtcgattctg
     1681 gccgccgact tcgcggagct cggtgacgaa gtcgccgcag tgaccggcgc cgactggctg
     1741 cacgtcgacg tgatggataa ccacttcgtg cccaacctga ccatcgggct gccggtcgtc
     1801 gaggcgttgc tcaagagaac cgacatcccg atggactgcc atctgatgat cgacaacccg
     1861 gacaaatggg cgccgcccta tgccgaggcc ggcgcataca acgtcacctt ccacgccgag
     1921 gccaccgaca acccggtcgg cgtggcccgc gacatccggg ccgccggcgc caaggcgggt
     1981 ctctcggtca agccgggtac gccgatcgag ccctacctcg agatcctgcc cgagttcgac
     2041 acgatgctga tcatgtccgt tgaacccggt ttcggcgggc agaagttcat cccggaggtg
     2101 ctggcgaagg tcggcatcgt gcgccggctc gtcgactccg gcgagctgac gatcgtggtg
     2161 gagatcgacg gcggcatcaa cgaagacacc atcgaagagg cggccctggc cggagtggac
     2221 tgcttcgtcg cgggttcggc ggtgtacagc gcagaggatc cggccgccgc ggtggaatcg
     2281 cttcgcaggc aagctgcctc ggcgtccaag catttgcgat tatgaatctc gacgccgcga
     2341 tgcggttggc cgtcgagcag gccgaccggg tcaagggcag cacctatccg aatccgcctg
     2401 tgggtgcggt cattttggac cgcggcgggg agatcgcggg cgtgggtgcg acggagccgc
     2461 ccggtgggcc gcacgcggag gtcgtggcgt tgcgcagggc ggggcagcgc gcgatcggcg
     2521 gtactgcggt cgtcacgctc gagccgtgca accactatgg cagaacgccg ccgtgtgtgg
     2581 acgcccttgt cacgtcgaag gtttcgcgag tggcttatgc tgtcgccgat ccgaacccgg
     2641 aggccgcggg cggggcggcg cggctcgccg aatccggcat cgaggtcgag gccggggtgc
     2701 tgaccgatgc cgtggcgggc ggtccgcttc gggaatggct gcacaagcag cgcaccgggt
     2761 tgccgcatgt cacatggaaa ttcgccacca gtgtcgacgg ccgcagtgcg gccgcggatg
     2821 gcaccagtca gtggatcacc agtgaggccg cacgggccga tgtgcatcgc cgccgcgccg
     2881 gagcggatgc gatcatcgtc ggcaccggaa ccgtgtttgt cgatgacccg gtgctgacgg
     2941 cccggctgcc cgacggtgcc ctggcggacc gccagccgct gcgtgtcgtc gttggagaac
     3001 gcgaaatatc ttctgaggca cgggttctca atgacgattc acgcacaatg gtgatccgca
     3061 cccgtgatcc gcacgaggtg atcaaggcgt tgtcggaccg caccgacgtg ctactcgagg
     3121 gtggcccgac gttggcgggg gcttttctgc gggccggggt gatcgaccgg atcctggcct
     3181 atgtcgcgcc aatcctgttg ggcggcccca tcaccgccgt cgacgacgtg ggtgtgctat
     3241 cgatcgcgca cgcgcagcgc tggcggttcg acggaatcga gccgatcggg ccggacgtgc
     3301 tgctcagtct ggtgtcggca taaccatcgc gggcggcgtc agcgccccga cctgatagag
     3361 cacatcgagc tcgtcgcgca caccccagtg ctcgcatagc cggccgtcgc gggagcggaa
     3421 gaagtgtgcg gtcttcatcg cgaaagaccg tcccgtcggc tcaatgccct gcggtgagga
     3481 cgcgttgacg ccgtgcgcgg tggcgcgaat cgccaccatg tcgccgtcgg cgatgacgtc
     3541 gtggacttca tagcggatct gcagcacggt cgtgacgaac gtcaggatcg cgatgtagcc
     3601 gtccggccct ggcggcagtg gaaccggtga gccgtggtcc acgaagtcgt cggtgaccag
     3661 gtcgcggatc ttgtccaagt gccccgcgtt catggcgtca atcagttcgg cgacaagctg
     3721 cgtggaggta tgcacatccc ctcaacgggc ggacggaccg ccgggttcag tcgctctcgc
     3781 tgccctcgtc attcatctcc agcgggcggt gttcgatgag gtcacgcacc ccgcccaggc
     3841 cgagccgttc ccggaaggcc cgcacggtgt agccgaccac cgtcgcatcg gtgcgcgccc
     3901 gcaccgtcgc gttgcgcggc atgtggaaga ccgggccgat ctcgccgaag taatcgccgg
     3961 aggtgacgac cttcagcaat tcctcgccac cgctggccaa ttcgcggagg atctcgagtt
     4021 caccctcgga cacgacgtag atcaggtcgc ccatcgtgcc ctgctcgaac aacacctcgc
     4081 cggcctccag ccggacagtg tcgggcggtc ggtccgtcga cgcgaagtcg ggaaccaact
     4141 cgacgacgcg atcggccaac ggcagaatcc ggctgtcgtg ggtcgcgacg accaccatgc
     4201 ggtcgccgga cgcgagttcg cggatcagcc gcaacacttc ctcgacctgg atgaagtcca
     4261 ggtgcgcggt gggctcgtcg gccaatacca ggggaggatc gagcgcgatc gcccgcgcga
     4321 ccgcgacgcg ctgttgctgc ccaccgctga gatcgcctgg ccggtgcgac atccggtcct
     4381 caaggccgac ccgggccaga agttgtcccg cccgtttgtg agccgcgcgt cgcgaaacgc
     4441 ccgccgcgct gagcggcacc atcacgttct ccatcgccgt gaggctgggc acgaggttga
     4501 acgcctggaa cacgatgccc acagtctcgc gacggtacgt cgacagcccg cgggcgtcga
     4561 gagcagtgac gtcgacgtcg ccgaacttga ttgcgccgtc gctcggtttg aggatgccgc
     4621 cgaggcacga cagcaatgtc gtcttaccgc agccgctggg gcccatcagg atcaccaatg
     4681 agcctgcggc cacatcgagg ttcaggccgt cgatcgggcg gacggtatct gcgccgctgg
     4741 cgtactcgac cgtcaggttc tggatggaca aatcgcccat ggctcaagga cctccgaagg
     4801 ccagcgcggg atcgacggac acggcgcgac gcagtcctgt cgcactggcg atcaagccga
     4861 tgacgacggc gatcacgggg agcgcgacga atgcctccgc gggcacgatg acctgcatcg
     4921 ggaacagcgg cccgagcagc cgcgagagcc cggcgccgac aagtgcggcc agcagcgcca
     4981 cgatgaccga ttgcagcgcc agcccggcgg cgatgttgcg agtaggaacg ccaatcgcct
     5041 tgaacaccgc gaagtctcgt agacgttcca gtgccgacag atagatcacc gatccgacca
     5101 ccaacgctgc gactacccaa agcagaaccg caacgatcgt gatcgagttc accgctacct
     5161 tcagcggccg caacaggtcg gcgatcgcgc cggctctgtc gatgccgcga tagccgtcgg
     5221 gtacctgtcc gagggtgccg cggataccga ccgacgcgac cagcggctgg ccgccatata
     5281 cgagtcgctg tgcaccttcg gtggtgagga agacattggg caggttggcc agcgcggtgg
     5341 agttctccac gatgccgacg atgcgcagtc tgcgcgaagc gatttcgacg tcgtcgccaa
     5401 tgcgccgtcc caatgtgctc gacacggcga cctcgtccgg ggtcgatggt ggccgaccct
     5461 ccgatacggc gggcatgccg ggcccgcgct ctggcgcgcc gaatatgtcg acattgcgcg
     5521 tcgtatcacc cagtgtggcg gtgccacccg cgtatgcgag cggtaccgcc gcaatcactc
     5581 ctggggacgc ggcgatccgt cgcaggtctg tgggcgcgaa cggggtggcc ccaacgaacg
     5641 ggcctgacgc gccggctttg accagaaaca gatccacgcc gagcgagtcg acggtcttgt
     5701 tggcctccac ccggaacccg ttggcgagtc cggtgagggc gagcgtcatc gcgaaaatga
     5761 tggacgtaga aaggatcgca atgacgaagc gccgtttccg ccactgcatg tcgcgcagcg
     5821 cggcgatcag catgtccgcg agattaccga cgccttaaca tccgcgggcg aagttcggct
     5881 atgacttgtc cttggcgaag gcacccacga gcgtgttgca aaacgcgggc aggtcgtcgg
     5941 gcttacggct cgacaccaac gtgtttggcc cgcgcgagca ttcgaccact tcgtcgtcga
     6001 cccaattggc gcccgcgttg cggagatcgg ttgccacgct cggccacgac gtcatcgtcc
     6061 ggccgcgcac cacatcggcc tcgatcagcg tccacggccc gtgacagatc accgcaaccg
     6121 gcttgccggc gtcgaagaaa ctctttacga acgcaaccgc cgcgtcgttg gtgcgcagga
     6181 agtcggggtt ggccacaccg ccgggcaaca ccagcgcggc gtagtcggac gacgaaaccc
     6241 tgtcggccgc cttgtcggcc tcgaacgtat cggccggcgt cagatggttg aacgcttgga
     6301 cctttccgac ttccgtggac accaactcgg gtgtgccgcc ggcctgctcg atggctttcc
     6361 agggttcggt gagttcgacc tgctcggcgc cttcgggtgc gacgaggaac gcaatcgtct
     6421 tgccgttcag tgaagttgcc atgaattctc gcccttcgaa ttgttgtcta aagcacgcgt
     6481 acccggcgcg gctagcaacc aatcaggggc tttgcgtcgg tagactcgta ccaaagcgct
     6541 ttcaccgacg agagttgcgc cgcctggtga gcactaacaa aggacaacga gcgtgactgc
     6601 actgcaggac tggctcagta ctcgatccgt agacccgaaa ggcgtcaagg cactcatctg
     6661 cgatgcgttg cgaagcctga ccgctgagga gccggcaaag gtggccgagc cgacattgct
     6721 ccgtcgggga atcgagcgct gctgagctag cgtcggtgcc ggcccggcgg aggctgctgt
     6781 gggatccgca ccgtctgatc cggcggatcc gggcgcgttg agatcgcctg ggtgggagcg
     6841 tcgatcagtg tggtcggggt gtcatccgcg tcgacagggg acactggggg cgcgggctcg
     6901 tcagcgtgtt catgccttcc gctgatcagc aggccgagca gggcgccgat cacgcagacg
     6961 atcgccgtga tgccgaagat ctcgccgtac tgcatcgtgt acgcctcgat ggtcgccgtc
     7021 ttcaagcgtg cgccctgggc gatcagtatg ccgcccggcg tgtcggcggt tggtgccggt
     7081 agcgcggcca gccgctcctg caggtactgg ttgaaccggt acagacccca ggcgctcagc
     7141 gcagcgatgc cgaccagcat gccgatcatc cgggccacca ccacgccagc cgatgcgatg
     7201 ccatgctggg ccgcgggcac gacccgtagc gtcgccgagg tcagcgggcc gatcaccagc
     7261 cccaggccga ggccggcgat cgccaggtcg gtgtccagca tgggcaggct gataatgccc
     7321 aggtggtgct tcgccgcgag gaggtcgaca ggccacttgg agatcagcca gtagccacct
     7381 gcggtgatca gcatgccggc gaacgccacg atgcggtcgc cgaccttggt agccagccag
     7441 ccgccgatca gcgcgccgat cggcagcgcg atcaagaagc gcagaagtag gaaggcggcc
     7501 tggtcctggt cttggcccag cacgccctgt ccgaagagtt cgacgttgac caacgtcacc
     7561 atcagggcgg cgccagcgca tagcgaagcc cccagcgccg ccaggaacgg tcggaagtgc
     7621 acgcccgcgg gttcgagcag ccgcgtctta gcgaccttct cccagacgaa gaacgccacg
     7681 gccgcgacca gggcgccgat gagcaccggc aggccccagc tcggcagcac ttgcttgccg
     7741 tccggcgccg ggttgtacaa gcccaccacg gtcaggccca gcgcgacggc cagcagcacg
     7801 cccccgacca cgtcgacttt ctccggatgg tcggtctgcg ccttcgacgg caggctgaaa
     7861 tggaccatca ccatcgcgat aactgcgaga gggacgttga cccagaacac ggcctgccag
     7921 tggttgaaca gccacaccag caggatgccg tacatcgggc cgagaacgct gcccagctcc
     7981 tgggcggcgc cgacaccgcc gagtacggcc gctctggtgc gcgccgccca aaggtcggcg
     8041 gccagcgcca gcgtgacggg cagcagcgcg ccgctggcgg tgccctgtac gacgcgcccg
     8101 atgaccaaca tggtgaggtc ggtggacagc gcggtgatca ccgacccgac cgcgaagccg
     8161 gccaggctga cctgtaacag cagcttgcgg ccgaaccggt cggaggcgcg gcccagcagc
     8221 ggcatggccg cgatatagcc gagcaagtac ccggtaatga tgggcgtgac ctgctggatc
     8281 tggttgatgc cgatgccgac gtctttcatg atgtcggtca tgatcgtgac gacgacgtag
     8341 gtgtccagcg cgcctagcac taccgcgagg ctgccggcgc tgatcgccac tctgcggctc
     8401 gtcgccgcct gtctggtggc ttcggaagcc tgcatcacac cgcaggcttg gtgacggtta
     8461 tctgcttgcc ccactcggtc gtggtcatcg tgatgctgtt atcggggctg acgtccagct
     8521 tcgcctgcgc cagttcgtgg ttgccgtcct cctgaatcca ggcggtggcg ggaacgggcc
     8581 cggtggctgc tatctgggga gcgatcttgt tgactgcgtc ggcggtgacg ttgcccgtca
     8641 cgcggacggt ctcgacgcca ttgatggtct cgcggccgtc ggccttgggg tcggtgaagt
     8701 tggccaggac gttcgccagc ccggtgtccg ggttgaggat ggccgacacg tcgtagatgt
     8761 cggcggcggg cccgaagtcc tggtaagccc cgccggacgt gagcgccgca aacaggttgc
     8821 catcggccac cacgaaaccg acatcggcca acttctggcc cagaaaaatc aggttcgcct
     8881 tgccctgtgc ggcgacggcg ggagcattgg tcagatcgcc ctcgagggtc tcgatcggca
     8941 gttccttgat ctcgccttgc acggccagct tcaggtgcac gctcgactgg ctcttggtcg
     9001 tctcggcgga ctccttgagc agcgtggcgg cgtctggcag ttccttggac gaatcctgcg
     9061 acttcgagca cccggcgatg agggcgatgg cggcgaaaag ggcggcgaag atcgccaaga
     9121 ggcgcgtctg catgagtgca tcgtagaggg tgctccgatg tgcacgcttt ccgcgggccg
     9181 ccggtagaag tgcaatcgtg gcttcggaac tggactttcg gcacgtatcg gccacgttct
     9241 tcggcgagac acagctatct ggttaatctg gctgcgtgtt caccggaatc gtcgaagaac
     9301 tgggcgagat cgtcagcaag gaagaactcg gcgactcggc ccgcttggtt atccgcgggc
     9361 ccgtcgtcac ttccgacgcc gggcacggcg attcgatcgc ggtcaacggg gtctgtctaa
     9421 ccgtggtcga ggtgttgcct gacggttcct tcagcgccga cgtgatgggc gaaaccctga
     9481 accggtcgag cctgcgcact gtcggcgtgg gcagccacgt caacctggag cgtgccgccg
     9541 cggtcaacag ccgcctgggt ggacacatcg tccagggaca cgtcgacggc accggccatg
     9601 tgatcgcgcg aacaccgtct gaacactgga tggtggtgcg gatcgcgctg ccgacggcgc
     9661 tatccaggta tgtcgtcgag aagggttcga tcactgtcga cggtgtctcc cttacggttt
     9721 ctggtctcgg ccacgactgg tttgaggtgt cgttgattcc gacgacgttg gacctcacca
     9781 cgctcggcag ggcggaggta gggacgcgcg tcaacctcga ggtcgacatc atcgcgaaat
     9841 atgttgaacg actgatgtct cccgataact cttctaccta gggttttctg ggctcgccat
     9901 aaaaggtcag cgtcagcgtc gatgacggag ccgttggccc tctggtgtgg gtttgtggtc
     9961 ggccgccacg ggtatccggt gcatgagggg tcgtgacatg cctaggcccg tccagaaagc
    10021 ggcggatcat ccaggctccg agcgtcgagt ccgtgcccgg caatgtgggc atttgggccg
    10081 cgggcggtga cgtagaccga ggagagttca cgatgaactt gaagaaaata gcagcaggtg
    10141 ccgcgatggt cggcgtgttg ggattcagtg ctgtggggtt cggtggtggt gcgcaagcca
    10201 aaccgcatgt ccccggtccc tgcgtaccgg gaatcgactg ttggattccg ggcgttgatc
    10261 cgcccggaca taacccgttt ggtccgcccg gacaagtcat gcaaggcaac ccggtggttc
    10321 ccggattgac aggagtcccg cccggtcact ggggtgaccc cacgttgttt gggctgcccc
    10381 cgacgtggct tccgggtaac tggatagacc ttgggattcc ggggccccta gatgtcgtat
    10441 ggaaccccga tcttgcggct tggggcatct ggtgggctgg ccagttcatc ccgtacgtgg
    10501 cgtaaacgac ccgactaccg gacggccgcc ctgcctctcg tagggcggcc gttgtcgtct
    10561 catggacccg aatccgcggc tgccccacta gatcccacgg taacgggcca atccctgtat
    10621 tcgaggctca acgcctccgg cggatagtgc tatgcaccga aaacgtcagc gccgaggtga
    10681 tgcgatcttc tttggctgtc caggtgagcg cagtgcctcg tcgctcggcg gccgcgccac
    10741 ctgaagcggc cctaatccgc catccgccct gttcagagag ctggcaataa ctgcacagcc
    10801 accgtggttc atactgatgt gatgacgagg ctggactccg tggagcgggc ggttgccgac
    10861 attgccgcgg gcaaggccgt tgtcgtcatc gacgacgagg atcgcgagaa cgagggagac
    10921 ctcatcttcg ccgccgaaaa ggccacccca gaactcgtcg cgttcatggt gcgctacacg
    10981 tcgggctacc tctgtgtgcc attggacggc gcgatctgcg accggctcgg gttgctaccg
    11041 atgtatgcgg tcaaccagga caagcacggc actgcctaca ccgtcaccgt cgacgctaaa
    11101 aacggtgtgg gaacgggtat ttcggcctcc gaccgtgcga ccacaatgcg tctgctcgcc
    11161 gatccgtcca gcgtcgccga cgaattcacc aaacctgggc acgtagtgcc gctgcgcgcc
    11221 aaagacggtg gcgtgttgcg ccgccctggc cacaccgagg ccgctgtcga cctggcccgg
    11281 cttgccggtc tgcagccagc cggcgcgatc tgcgagatcg tcagccagaa ggacgaaggc
    11341 gcgatggcgc agaccgatga gctgcgtgtt ttcgccgacg accacaacct ggcactgatc
    11401 tccatcgccg acttgatcga atggcggcgc aagcacgaaa agcacatcga gcgcgtcgcc
    11461 gaggcccgca tccccacccg gcacggcgaa ttcagggccg tcggctacac cagcatctac
    11521 gacgacgtcg agcatgtggc gctggtccgc ggggacatcg ccggaccgca cggcgacggt
    11581 cacgatgtgc tggtgcgcgt gcactcggag tgcctgaccg gtgacgtgtt cggctcccgc
    11641 cggtgcgact gcggcccgca actcgatgcg gcgatggcga tggtggcccg cgagggccgc
    11701 ggcgtcgtgt tgtacatgcg tggccacgag ggccgcggca tcggcctgat gcacaagctg
    11761 caggcctatc agctgcagga cgccggcgac gacaccgtcg acgccaacct caagctcggc
    11821 ttgccggcgg acgcccgcga ctacggtatc ggcgcgcaga tcctggtcga cctcggagtg
    11881 cggtcgatgc ggctgctgac caacaaccca gccaagcggg tgggtctgga cggctacggg
    11941 ctacacatca tcgagcgggt tccgttgccg gtgcgcgcca atgcggagaa catccgctac
    12001 ctgatgacca agcgcgaccg gatgggccat gacctgatcg gccttgacga ctacgacgaa
    12061 gccgtgccgg gcgagttcgg cggagccctg tgagcggcca gagcggagct ctgaattgag
    12121 cggaggcgca ggcgtcccgg atctgccgga tctggacgca tcggggttga cgctgggcat
    12181 cgtcgccagc acgtggcacg agaaagtatg tgatgcgctg ctcgagggcg cacgcaaggt
    12241 cgccgcggac gcgggtatcc acaatccgac catcgtgcgc gtgcttggcg cgatcgagat
    12301 ccctgtcgtg gcacaggcac tggccgccaa tcacgacgcc gtcgtggcgt tgggcgtggt
    12361 gatccgcggc gagacaccgc atttcgacta cgtgtgtgac gcagtgaccc agggcctgac
    12421 gcgggtgtcg ctggatgcgt cgactccggt ggccaatggt gtgttgacca cgaataccga
    12481 gcagcaggcg ctggatcggg ccggactgcc ggactccgcc gaagacaagg gtgcacaggc
    12541 ggccgccgca gcgctgtcga cggcgctgac gttgcgcgat ctgcggtcat gagcgactgg
    12601 gacatcgaga tacggcccca tctgacaccg tatttcgcct acgcggcggc cgcggtaatc
    12661 ctggctgttc atatcgccgt tggtgcgctg ctgaagatct cctcgacggg tgtggtcttc
    12721 cagaccgccg atcaggtggc aatcgggctg ctgggtgggg tcatcgccgg cgccgttttg
    12781 ttgttcaccc gaccgcggct tcgcgtcggc agcgccggtg tcgcggtgcg aaacctgttt
    12841 ggttacaagc tgatcccgtg gtccgatgtg gtaggcgtgt cgtttccgcg cggagcgcgg
    12901 tgggccaggg tagacctgcc cgacgacgag tacatcccgg tgatggcgat ccaggccgtc
    12961 gacaaagagc gtgccatcga cgcgatggac cgcgtgcgcg ggctgctggc gcactaccgt
    13021 cctgacatca actcacactg agtgtcatcg cgacttcacc acgggcgctc ggggtctcgg
    13081 tcgcattgag ggtgaatcca agggcttcgt acacgccgcg ggccaccgtg ttgtccgctg
    13141 cgacccgcag cgtgacggtg cgcgctcggc ggtcgcgggc ccagtccaca gcggcggcca
    13201 ccagcggccg cgccaagccc cggcggcggg cggtcgggtc gagccacaag gagtacaggt
    13261 agacggtttc ggcgctttcc agctgcgcgc cgatcagccc gacgggtcgg ttgtcgatca
    13321 gcaccgcgaa ttgagcgtga tcgcgcagcc tgcggcgcca ctgtgcggca gtgaacgcta
    13381 cctcgtgttg atactgcggg tcgttctcgc ctagggagtc ggtcaacgcc cgcaaccgaa
    13441 ccccggcgaa cacccgccag tccgcctcgg tcagcctggc gacgcgggcc gtcgcggctg
    13501 tcaccgagtg tcgcccatga ccaggccctc acggcggggg tcagcgccac cgacccaacc
    13561 cgcgacatcg cgcgcgatcg ccgacagtcc gctggactgg tcagccaggt cgacctgatg
    13621 cccgagcttc cgcaggcctt gcaccagcgg atcatggtcg ccgttgtcgg agatgtcgat
    13681 gttcgggtgt tctccgccga cattggtctt cggtgtgttc gccgcaccga agtccaccgc
    13741 ggacgccgcc tgctgcggat cgaggcccca gtccaccatt cccacaacag ttttcacgac
    13801 aaactggata atcaccgagc cgccaggcga gcccaatacg gcgtacagcg ggcctttggc
    13861 cccgggctgg gcaccgcggt cgaacacgag cgtgggcgcc atcgtgctgc gcggccgctt
    13921 tcccggctgc acgcggttgg ctaccggcac cccgtccttg acgggctcgg cggagaagtc
    13981 ggtgagctga ttgttgagaa tgaatccgtc caccatgtgg aacgagccga acgccgactc
    14041 aaccgtcgtc gtcatggcgg ccgcgttgcc ctgcgcgtcg acgatgctga tctggctggt
    14101 gccgtgttcg ggcgtcggcg gcgctggcgt cgtcggcgga ccgaactcac cgggcttcgc
    14161 ggtgcccatg gtgtgctgtt cggagatcag agccgcccgg ccggcgaggt agtcactgcc
    14221 cagcaatgtg ttgggcgagc cgcctggcag cggcacgaaa tcagtgtcgg caacgtactt
    14281 gtcgcggtcg gcgtaggcga gccgttcggc ctcggagatc agatgcacac ccatcacggc
    14341 gggcttgccg ccgttgaggt cgacgtccgt gggtttgtga tcggccatcg cgaaatgctc
    14401 aagcatgacc agcgtcgcgg ccaccgcaat gccacccgag gacggcggcg gcatgccgca
    14461 gatttccttg ccgcggtacg gagtgcacag cggttcgcgt ttcttggcgg cgtatccgga
    14521 cagatcctcg agcgtcatca ggctcggggt gcggccaccg gcggtatccg atgcggcggc
    14581 gatgatgtcc cgcgcgatgt cgccggtgta gaacgcctgc ggatcagatg cgatggcgcc
    14641 caacgtcttt gaatagccgg gattggtcag ccgggtcccc gacgtcttcg cgctgccatc
    14701 ggagttgagg aagtaggccg aggcattcgg atccaccttc agtttcgggg ccgcgtcagc
    14761 gattgcggct gccagccggg ggctgatctc gaagccgtcg tcggccatcg ccaccgcggg
    14821 ggtgaacagg tcacgccagg cggtcttgcc gtgctcggtg tggacgtcct gcagcagccg
    14881 caagatgccg ggcacgccga tggaccggcc ggacgcgcgg gcgtcgggct tgggctcggt
    14941 gcggtcggca tcggagatcc agcgcaggta gttctcggtg gccgcgttgg gcgccacctc
    15001 gcggccgtcg taggcctgca cggtgcccgc ggcggcgtcg tagtacagca ggaacccgcc
    15061 gccgccaatg ccggaggact gcggctcgac cagtccaagc actgcctgcg ccgtcacgag
    15121 ggcgtcggcc gcggttccgc ccttgttcaa cacctgacac gcggcctggg tggccaacgg
    15181 gttggcggtg gccaccgcgt agtgggcggt cttcaccgcg gtcatgtcct tgcggtagcc
    15241 ggtggcgacc tcggggttgg tggagatgtt cttcgacgtg gtgaccgacg ccgtcgccgg
    15301 cgccgcggag gtcttgggag ccggcgtgcc gttggggatg accgagcacg gacccgccac
    15361 ggttgtcccg gtgggcggtg cgttctcctc ggcagcacat ccggccagta ccaacacgac
    15421 cccggctagc ggggccacga cctttctcag gagcgggatc cgcatcactc gacggtagtt
    15481 catcggaaag gatcgggttg acgctatcgc ccgcgcgccc ctacgcctcc tccgcccggc
    15541 tcatccgctg gccgagttcg gcccgcgagc ggataccgag cttgcggtag atacgtgcga
    15601 ggttcgcttc cacagtcttc ggactcagga acaacgcggc agcgacgccg cggttggtca
    15661 ttcccgacgc cgccagttcg gcgacgcgct gttccgacgg ggtcaggtcg acgccgccga
    15721 tgcgcgcggc atgcgcgcgg gcgagttcgt ctcgcgtccg atccgcccac agcggaacac
    15781 ccatctcttc gaacgctgcc agcgcctcct gcaggatctc ggcggcggct ttccgttggc
    15841 gctgcctgcg atacagctga cccgccacca actgggttcg cgcgcgttcg aacggcatcg
    15901 acagccgcgc gtgttcgctg attgcggttg cggcggctgc gctggcccct tcaatatctc
    15961 cttgcgctgc aagcaacatg gcgcggcagc gggcgccaac agccaacatc cacggccggt
    16021 cgagtcgatg gccgttgcgt tccagcgctg caacgagtgg ttcggcgtcg gctatccgac
    16081 ccacctgaat catcgcctcg acggcgtcgg ggatgaacgc cgctgtgaat atctcggtac
    16141 ccaccgtatc gagcgcggca agcagggggt ccagcacgtg aagcgcgctg ccgtagttac
    16201 ccagcgacac atcgagaaat cccagggtga tggcgggcca tgacgccagc agcgaggacc
    16261 cgcagcgacg gctggccacc aacccttcgg cggcgtcctc agccacccgc gaggcctcag
    16321 tgaagtccgc tcgccagacc tcgagtaaaa cgctgtggaa ggccgcgtag atcacttcgt
    16381 tgtcctcacc gcgctcgatg tagtgacgcc ggatggattg catctcctcg tgagcgaggg
    16441 tgaggtcgcc tgcccaaccc cgaagtatcg cgttctgtag gcgtgggcgg aatgcggccg
    16501 gtatatcagg ttggcgccct tccaattcga ctgcgcgatg cagatcggtt tcgtccagcc
    16561 cgcctccgcg catgcatcgc accaaggctc taatgccaag cgcctgacta agtaagtgtg
    16621 gttggtccaa tcgggtcgct gttgccacag cgtcttcgac actgcgtgcg gcactttcca
    16681 gctagccgaa gttgagccgc gcgaacgaca gtgcgatgag tacctgcgct cgatgtgagt
    16741 ggtcctcgcc ggactcgttc aacgcttgtt tcagcaagct ggccgtctcc gggaaaatcc
    16801 cgttgagcag gcccatgact gcgaggagac tgaccgcgtc tgctcgtagg tggtccggcg
    16861 acaagtcgcg gatggttacc tcaagcaacg cccgggccct ccccgggtgg ccggcactga
    16921 aatggtagag agccaaccgg attcggcgct cgggagtgtc accgcctagt ccgaaagcca
    16981 aatcaagcag ttccaccgct gccgctggcg ctccccgaag acgcgcggct tccgcacccg
    17041 catcgaggga ctgcagtgtt gcccgatcgc cgctcgttgt ggcgagggca aggtgcctcg
    17101 cgcgcagttc gggttcgtcg aagattgcgg cgaggcgccg gtgcatccta cgtcgctgcg
    17161 ccgcaggcac attcgtgtac atgccgtgcg ccaacaaggg gtggacgaac cgcactcggt
    17221 ggcccacgag cgtcacgatt ccgttgtcct cggccttctc cagcagctcg gcggcgcggt
    17281 gcgagtcggc gtcaatcgcc cggccgacga cctccaccgt cggggcggcg acacacactg
    17341 tggccaagag cgcgtcctgt accgcaggat cgatgctgca atccgagccg cgtgatcgca
    17401 tccggccgcg gcatctgcag ccaagaagcc ccggttgagc tggtcttgtc ggtgcgaagt
    17461 gtgccaagca caccgacacg tgtcgaaagt cggcgcgcca cataggcaag gacgtctctg
    17521 ctcgaggtgt cgagccattg aagatcgtcg atcgccagga gggtgggcga ctcggcggca
    17581 atgcggttca ccagcgatag gaagcccgcc gcaagcgtgc gctgattggt agatcgcccg
    17641 tccgatcgca gcagcatccg gtccagcgct tcccgctggg gtgagggcag atcgccccag
    17701 aaaatttcgt cgacatcggc aagcaggtca gccgcggagg tataggcgcg taccgattct
    17761 gtctgagccg tgcgggcgga aagtacgcga aacccgcgtt ctcgggcttg gtcgatcgcc
    17821 gccaaccaca gcgtggtctt cccgattccg acctcgcctt cgaccaccaa agcag
//
LOCUS       DASRJA010000285         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2948440,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000285 DASRJA010000000
VERSION     DASRJA010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2948440"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2044
                     /gene="fdhF"
                     /locus_tag="VFC01_12570"
     CDS             <1..2044
                     /gene="fdhF"
                     /locus_tag="VFC01_12570"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HZN80505.1"
                     /translation="ELVRMVPYKDGGANEGHSCVKGRFAFGYATHPDRKLRPMVREKI
                     TDPWREVEWDEAIGTVARRMLEIRQRHGAGAIGAITSSRCTNEEVYVVQKMVRAAFGN
                     NNVDTCARVCHSPTGYGLKQTFGESAGTQDFRSVAQSDVIMVIGANPTDGHPVFASRM
                     KQRLRQGAKLIVVDPRRIDLVRSPHIEADHHLQLKPGTNVAVVNAMAHVVVTEGLVDQ
                     SFVGERCEGFDEWAQFISGTENSPEAVESITGVPASELRAAARLYAQGGNGAIYYGLG
                     VTEHSQGSTMVMGMANLAMACGNIGRDGVGVNPLRGQNNVQGSCDMGSFPHELSGYRH
                     VSDDAARKVFENLWGTTLLAEPGLRIPNMFDAAIEGTFRALFVHGEDIAQSDPNVKHV
                     LAALGAMELVVVQDLFLNETAKFAHVFLPGASFLEKDGTFTNAERRINRVRAVMKPKS
                     GRHEWDIVCQIATAMGYPMQYDHPSQIMDEIASVTPTFTGVSFDKLDEMGSVQWPCNE
                     QAPDGTPIMHVDAFTRGLGRFVPTPFVPTDERSTRRFPLILTTGRILSQYNVGAQTRR
                     TANIAWHQEDVLEIHSHDAEVRGIEDGDEVTLASRVGATTLHAQISDRMPTGVVYTTF
                     HYPVTGANVVTTENSDWATNCPEYKVTAVQVTLAHPVSHLNGEAAADRELAVGLVD"
     gene            2046..2258
                     /locus_tag="VFC01_12575"
     CDS             2046..2258
                     /locus_tag="VFC01_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HZN80506.1"
                     /translation="MSHDEATEIRLINKIVVHFAYLPTEEAVIEVANHVSRFWDPRMK
                     RRLFELVDSETKDFEPVAVAATALLR"
     gene            complement(2527..3144)
                     /locus_tag="VFC01_12580"
     CDS             complement(2527..3144)
                     /locus_tag="VFC01_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80507.1"
                     /translation="MPTTAPEKLTPKGRATRERIVAAAAELMSRRGVARTTIEDIQEA
                     AAVSTSQMYHYFADKGDLVAAVIDFQTDRVLAVQHLGLDRLECLEDLRRWRDIMVDIM
                     RRLGFVGGCPIGSLANELAETDPLARAQVARSFVQWETMIRDGLKAIAARGELPDGTE
                     VDNLALATLAAIQGGLLLSQVRRDTAPLEAAVDTMIEHLRTLGVR"
     gene            3420..>3603
                     /locus_tag="VFC01_12585"
     CDS             3420..>3603
                     /locus_tag="VFC01_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AhpC/TSA family protein"
                     /protein_id="HZN80508.1"
                     /translation="MTTAANTNTTTIADRVAKMDAGRAGQIPDEVLGVFGAEQAGLDA
                     RGIPTGIAEPGDTLGDF"
BASE COUNT          685 a         1099 c         1185 g          634 t
ORIGIN      
        1 cgagttggtc cgcatggtgc cctacaagga tggcggcgcc aacgagggcc actcatgtgt
       61 gaagggacgc ttcgccttcg gctacgcaac ccatccggat cgcaagctgc ggccgatggt
      121 tcgcgagaag atcaccgacc cgtggcgtga ggtcgaatgg gacgaggcga tcggcaccgt
      181 cgcccgccgg atgctggaga tcaggcagcg gcacggcgcg ggggcgatcg gcgcgatcac
      241 ctcgtcgcgg tgcacgaacg aagaggtcta cgtcgtccag aagatggtcc gcgccgcctt
      301 cggcaacaac aacgtggaca cgtgtgcgcg agtttgccac tcgcccacgg gatatgggct
      361 caagcagacg ttcggtgagt cggcaggcac gcaggacttt cgttccgtgg cgcaatccga
      421 cgtcatcatg gtgatcgggg ccaatccgac ggacgggcac ccggtcttcg cgtccagaat
      481 gaagcagcgg ctgcgccagg gcgcgaagct catcgtcgtc gacccgcgtc gtatcgacct
      541 ggtgcgttcg ccacacatcg aggccgacca tcacctgcag ttgaagcccg gaacgaacgt
      601 ggcggtcgtc aatgcgatgg cgcacgtggt ggtgaccgag ggcctcgtcg accagtcgtt
      661 cgtgggggag cggtgcgagg gattcgacga gtgggcccag ttcatctcgg gcaccgagaa
      721 cagccccgag gccgtcgagt cgatcaccgg cgtgccggcc tcagagctgc gtgcggccgc
      781 acggttgtac gcacagggcg gcaacggggc catctactac gggttggggg tgaccgaaca
      841 cagccagggc tcgacgatgg tgatgggcat ggcgaacctg gcgatggcgt gcggcaacat
      901 cggccgcgac ggtgtcggcg tgaacccgct gcgcggccag aacaacgtgc agggctcctg
      961 cgacatgggg tcgttcccgc acgagctgag cggctaccgg cacgtgtcgg atgacgcggc
     1021 gcgcaaggtc ttcgaaaacc tatggggaac aacgctcttg gcggagccgg ggctgcgcat
     1081 tccgaacatg ttcgacgccg cgatcgaggg aaccttccgg gccctcttcg tccacggtga
     1141 agacatcgcg caatccgacc ccaacgtgaa gcatgtcctt gccgcactgg gtgcgatgga
     1201 gctggtggtg gtccaggacc tgttcctcaa cgagaccgcg aagtttgcgc acgtgttcct
     1261 gcctggtgcg tcgttcctgg agaaggacgg caccttcacc aacgccgaac gccgaatcaa
     1321 ccgggtccgt gcggtgatga agccgaagag cggcaggcac gagtgggaca tcgtctgcca
     1381 gatcgccacg gcaatgggct atcccatgca gtacgaccac ccgagccaga tcatggacga
     1441 gatcgcatct gtcacgccga cgttcaccgg agtgtcgttc gacaagctgg acgagatggg
     1501 cagcgtccaa tggccgtgca atgagcaggc tcctgacggc actccgatca tgcacgtcga
     1561 cgcattcacc agggggctgg gcaggttcgt gccgaccccg ttcgtgccga ccgacgagcg
     1621 cagcacacgc cgcttcccgc tgatcctgac gacgggccga atcctgagtc agtacaatgt
     1681 cggagcgcag acccggcgca cggcgaacat cgcctggcac caagaggatg tgttggagat
     1741 ccattcgcac gacgccgagg tgcgcggcat cgaggacggc gatgaggtca cgttggccag
     1801 ccgagtcggc gcaacgacgt tacacgcgca gatctccgat cggatgccga ccggcgtcgt
     1861 gtacaccacg ttccattacc cggtgaccgg agcgaacgtc gtgacaacgg agaactccga
     1921 ctgggcgacg aactgccccg agtacaaggt gacggctgtt caggtcacgc tcgcccatcc
     1981 ggtctcgcat ctgaatggcg aagcggccgc cgaccgtgag ctggccgtcg gactcgtgga
     2041 ttgacatgtc gcacgacgag gcaaccgaga tcaggctgat caacaagatc gtcgtccatt
     2101 tcgcctacct gcccaccgaa gaggcggtca tagaggtcgc caaccacgtc agtaggttct
     2161 gggatccacg catgaaacga cgactgttcg agcttgtcga ctcggagaca aaggatttcg
     2221 aacccgttgc ggtggccgcg acggcgctgc tgcgctagcc gttacgagtg acgtagcact
     2281 tgagaaggaa gtgatcgaga ctcgctgact gctcagtttt gacgcgcgta cagccgatgg
     2341 gcgcccccga gttcgcgggc aatgtcgttt tttttggggc tgtctcactg cggtttgaac
     2401 atcgtgaatg aggcaaacta tttgtgcgac agagtattga gacagctcga cctgggcgaa
     2461 tcggccaact ccgtgcacgt cggcaatgtc tcaaatggtc tatttcgaga cgccagtacg
     2521 gggaatttac cggacgccca gcgtgcggag gtgttcgatc atggtgtcga cggccgcttc
     2581 cagcggagcg gtgtctcggc ggacctggct gagcagcagg ccgccctgga tggcggccag
     2641 cgtcgcgagt gccaggttgt cgacctcggt gccgtcgggt agttcgccgc gagcggcaat
     2701 ggccttgagc ccgtcgcgaa tcatggtctc ccactgaaca aacgaccgcg cgacctgcgc
     2761 gcgggctagc gggtcggttt cggcgagctc gttggccagc gatccgatag ggcagccgcc
     2821 gacaaagccc agccgccgca ttatgtcgac cataatgtcg cgccaccggc gcaggtcctc
     2881 aaggcattcc aggcggtcca gacccagatg ctgaacggcg aggacccggt cggtctggaa
     2941 gtcgataact gctgcgacca ggtcgccttt gtcggcgaag tagtgataca tctgcgaagt
     3001 gctcaccgca gcggcttcct ggatgtcctc gatggtggtg cgggccaccc cacgccggga
     3061 catcagttcg gcggcagcgg ccacgatccg ttctcgggtc gcgcggccct tgggggtcag
     3121 cttctctggt gcagtggtag gcatcggagc ggctttctac tggagcgatc gcatgctgtg
     3181 ggttggggtg cgaactgacc cgtcacatca gcctctcgtc gatctcgtca tggtcttctc
     3241 accattggag cccgatgaaa atggagtgtc aactccaaaa tctaatgcac cgagtgctca
     3301 gccgacactt tttggagttg acactccatt tttctcgatg caagctcgcg tcgtctcgca
     3361 agttcgaacc ctgccggaaa ccgttgtgtg gcaccggcac acatctgaaa ggctctttga
     3421 tgacgaccgc tgctaacacc aataccacca cgatcgccga tcgcgtcgcc aagatggacg
     3481 cggggagggc gggccagatc cccgacgagg tactcggtgt gttcggcgcc gagcaggccg
     3541 gcctggatgc ccgcggcatc cccaccggca tcgccgaacc cggcgacacg ctcggcgatt
     3601 tcg
//
LOCUS       DASRJA010000286         4102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2319353,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000286 DASRJA010000000
VERSION     DASRJA010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2319353"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VFC01_12590"
     CDS             complement(<1..789)
                     /locus_tag="VFC01_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005261929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN80509.1"
                     /translation="MRVVDNRREIREFLTTRRARITPEQAGLPAYGGSNRRVKGLRRE
                     EVAMLAGVSADYYIRLERGSLVGASDSVLDALARALQLDDAEREHLFDLARIDNAASA
                     KRRVSSSRQRIKPVIQQVLDAITEAAADVRNDRGDILASNRLGYALYSEIHAESVQPP
                     NSARYTFLNPSAREFFVDWDKAATDVVALLRATVGRNPYDKPLSDLVGELSTRSEEFR
                     VRWAAHNVRRHTSGSKRMRHPVIGEIELNYQSFELPGEPGLRFNV"
     gene            904..1883
                     /locus_tag="VFC01_12595"
                     /pseudo
     CDS             904..1883
                     /locus_tag="VFC01_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            1931..2779
                     /locus_tag="VFC01_12600"
     CDS             1931..2779
                     /locus_tag="VFC01_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN80510.1"
                     /translation="MQTVALNNGVEMPILGFGVYQVPAEDTEQVVAEALSTGYRLIDT
                     AASYQNEEAVGRALNTSGLAREELFVTTKLWMEHAGYDATKAAFHRSLDRLGLDYLDL
                     YLIHQPFNDYYGAWRAMQELNQQGLVRAIGVCNFHPDRLVDLIEHNDVTPAVNQIETH
                     VYYQRGEDHRIMCEYGVAHQSWGPFAEGKNNMFIDPTLASIGAAHGRSVGQVVLRWLI
                     QRDVIAIPKSVRPERMRENFDIFDFELSDEEMNRIAVMDAGASLFFDHRDPSIVSQFS
                     KRHIDV"
     gene            2796..3989
                     /locus_tag="VFC01_12605"
     CDS             2796..3989
                     /locus_tag="VFC01_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN80511.1"
                     /translation="MINSDNPTGHARRRFLMGAAGLAAAPLVAGIAAGCSSGERNQDS
                     GGDNTDDAGNPVTAATTVTGRRKLGSLEVSSIGLGCQTMPGTLYGPVTSRADMVTLIR
                     TAVDQGVTFFDTAEAYGPFESERIVGEALQPVRDQAVIASKFGFDIDPQTGQRRGGPN
                     SRPDHIRQSVDAMLQRLRTDRIDLLYQHRVDPTVPIEDVAGTVKDLISQGKVTHFGLS
                     EPGLETVRRAHAVQPLTAIQNEYSMLWRGPEQHVLPLCEELGIGFVPWAPLGMGFTTG
                     TINPSTRFAKGDFRAAVPRNSPQNLAANMPLVQLIQDWAVRKGATPAQISLAWLMAQH
                     PWIAPIPSTTRIAHLVENLGVEEVTFSDEELADLNTGLAAISIHGDRLQPAVLAQTGV
                     EAPLQ"
BASE COUNT          779 a         1358 c         1278 g          687 t
ORIGIN      
        1 gacgttgaat cgcagtccgg gctcaccggg caattcgaag ctttggtagt tcaattcaat
       61 ttcgccgatg acggggtgcc gcatgcgttt ggagccactg gtgtgtcggc ggacgttgtg
      121 ggccgcccaa cgcacgcgga attcttcact gcgcgtggat aattcgccga caagatcgga
      181 caacggcttg tcataagggt tacggccgac cgtggcgcgc aggagcgcaa ctacatcggt
      241 ggccgctttg tcccaatcga cgaagaattc tcgagcactc gggttcagga aggtgtaacg
      301 cgccgagttg ggcggttgca ctgactcggc gtggatctcc gaatacaggg cgtagccgag
      361 ccgattcgac gccaagatgt cgccgcggtc gttccgcacg tccgctgccg cctcggtgat
      421 cgcgtcgagg acctgctgaa tcacaggttt gatccgctgc cgcgacgacg aaacgcgtct
      481 cttcgcgctg gcggcgttgt cgatgcgggc caggtcgaag aggtgctcgc gctcggcgtc
      541 gtcgagttgc aaagcacgtg cgagggcgtc gagaacgctg tcggacgccc cgaccaggct
      601 gccgcgctca agccggatgt agtagtccgc cgaaaccccg gcgagcatgg cgacctcttc
      661 gcgccgcaaa cccttgacgc ggcggttgct gccgccatag gcgggcagtc ccgcctgctc
      721 cggggtgatg cgggcgcgcc gagtggtcag gaattcgcga atctcgcgcc ggttgtccac
      781 gactctcagg ctagggcgct taaagcgcag cgtgggggtg tctgccagta cctgtctgga
      841 cagacactcc cccgtaggcg tcgttgccgg ttgcctggga attgcaatgg atggaggaca
      901 agcgtgaagt acatcaacct cggggatctg acggtatctc gcatcggcct gggcgcgatg
      961 ggcatgtcgc atggctacac cggagcgggc agcgacgaca ccgagtccat ccgcaccatt
     1021 caccgggcgc tcgatctggg cgtcaccttc atcgacaccg ctgagatcta cgggcccttc
     1081 gtcaatgagg aactcgtcgg tcgggcgttg aagggccgcc gcgacggcgt cgtgttggcc
     1141 acgaagttcg gcttggtctc gcatgccggt gagggtgcgt ggaaccttga cagcagcccg
     1201 gccaacatcc gcaccgccgt cgagggttcc ttgcggcggc tcggcaccga ctacatcgac
     1261 ctgtactacc agcaccgcgt cgatccggac acgccgatcg agaagacggt cggcgcgctc
     1321 gccgagctgg tggccgacgg caaggtctgt cacatcggcc tgtccgcggc cgcggcgaat
     1381 acgattcgtc gcgcccacga ggtgcacccg gttacggcgt tgcagtcgga gtattcgctg
     1441 tgggcccggg atttggaggc cgagatcctg ccggcgctgc gtgaactggg catcgggcta
     1501 gtgcccttct ccccgctggg ccgcggcttc ttgaccggca cgatccgctc gcccgagcag
     1561 ttcgccgacg acgatgactt ccgcaagacc aacccccggt tcactggcga gaacttccgg
     1621 cgcaacctgc gcatcgccga cgaggtgcaa gccgtcgcca ccgaggtggg cgcgacccgg
     1681 ctcaggtggc actggcatgg ttgctggctc aaggcgacga cattgctccc ataccgggca
     1741 ctaagcgggt ttcccccgtc gaggagaacg ccgctgccga tggtgtcgaa ctcagcgcca
     1801 cgcagatcga cacactcaac aacctcacgc ccgcggctgg cgagcatcac accgcagaac
     1861 aaatgcagat gtacgagcgc tgagcgccca ccgctgtgca cgagaccgcc tcgacaagaa
     1921 ggactttcag atgcagaccg tagctttgaa caacggtgtc gagatgccga ttctcggttt
     1981 cggggtctat caggtgcccg ccgaagacac cgaacaggtt gtcgccgagg cgctgagcac
     2041 cggctaccgg ctcatcgaca ccgctgcgtc ctaccagaac gaggaagcgg tcggacgtgc
     2101 actcaatacc agcggtcttg cgcgcgagga gttgttcgtg accaccaagc tgtggatgga
     2161 gcacgcaggt tacgacgcga cgaaagccgc attccacagg tcgctggacc ggctgggcct
     2221 ggactacctc gatctctacc tcatccacca gcccttcaac gactactacg gtgcctggcg
     2281 agcgatgcaa gaactcaatc agcaaggcct ggtgcgcgcc atcggggtgt gcaactttca
     2341 ccccgaccgc ctcgtcgacc tcatcgaaca caacgacgtc acgcctgcgg tgaaccagat
     2401 cgagacccac gtgtactacc agcgcggcga ggatcaccgg atcatgtgcg agtacggggt
     2461 ggcgcaccaa tcgtggggcc ccttcgccga gggtaagaac aacatgttca tcgacccgac
     2521 gttggcctcc atcggggcgg cacacggcag gtcggtcggc caggtcgtgc tgcgctggct
     2581 gatccagcgt gacgtcatcg cgatccctaa atccgttcgg cccgaacgga tgcgggagaa
     2641 cttcgacatc ttcgacttcg aactcagcga cgaggagatg aaccggatcg ctgtcatgga
     2701 cgccggggcc agcctgttct tcgaccaccg cgatccctcg atcgtcagcc agttctccaa
     2761 acgccacatc gacgtctgac atcagaagga cacgaatgat caacagcgac aacccaaccg
     2821 gtcatgcgcg gcgcaggttc ctgatgggcg ccgcgggatt ggccgcagcc ccgctggtcg
     2881 cagggattgc cgctggctgc agttccggcg agcggaacca ggactcgggc ggcgacaaca
     2941 ctgacgacgc cggcaacccg gtaaccgcgg ccaccaccgt gaccggccgc cgcaagctgg
     3001 gctcgttgga ggtttcgagt atcgggctgg gctgccaaac gatgcccgga accctctatg
     3061 gtccggtcac cagccgcgcc gacatggtca ccctcatccg cacggcggtc gatcaaggtg
     3121 tgacgttttt cgacaccgca gaagcctacg gtccgttcga gtccgaacgc atcgtcggag
     3181 aagccctgca acccgtgcgc gaccaggcgg tcatcgcctc caagttcggc ttcgacatcg
     3241 atccgcaaac cggccaacgc cgaggcgggc ccaacagccg ccccgatcac atccggcagt
     3301 ccgtcgacgc gatgctgcag cgcctgcgaa cagaccggat cgacctgctc taccaacacc
     3361 gcgtcgatcc gacggtgccc atcgaggacg tggccggcac tgtcaaggac ctgatctcgc
     3421 aaggcaaggt cacccacttc gggctctcag aaccgggact ggaaacagtc cgtcgcgcac
     3481 acgcagtcca accgctgacg gccatccaga acgagtactc catgctgtgg cggggacccg
     3541 agcagcacgt ccttccgctg tgcgaggaac tcggcatcgg cttcgtgcca tgggcgccgt
     3601 tgggcatggg attcaccacc ggcaccatca atccgtccac gcggttcgcc aagggagatt
     3661 tccgggcagc cgtgccgcgc aacagcccgc agaacctggc cgccaacatg ccgttggtgc
     3721 aactcattca agactgggcc gtgcgcaagg gcgccacccc agcgcagatc tcgctggcat
     3781 ggttaatggc ccaacaccca tggattgcgc ccatcccgag caccacccgc atagctcacc
     3841 tcgtcgagaa cctcggtgtc gaagaggtca ccttcagcga cgaggagctt gcagacctca
     3901 acaccggtct cgccgcgatc agcattcacg gtgaccgctt acagccggcc gtgctcgcgc
     3961 agactggcgt cgaagcgccc ctccagtaac ggttctcacg cctatgaatc gccgcacgct
     4021 catgaagggc tcgatcacgc tcggagccgg ggcagtgctc agcgcgcagc tcgccagttg
     4081 cagcccacca ccagcacaac gc
//
LOCUS       DASRJA010000287         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3709167,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000287 DASRJA010000000
VERSION     DASRJA010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3709167"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="VFC01_12610"
     CDS             <1..1234
                     /locus_tag="VFC01_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80512.1"
                     /translation="RLRLYLTGAMAVSTLGLLAAAVTGLAELLLLFLTCGGCGIFLAV
                     FFDRLSLYRVLAAMVSPQVPMRDLAPALPAHIEARLEALNKSNVVIFEGGDAFIAWGR
                     RLRDGWQMNIDATRAATDTNGKARTVIPFTPVDLHKAVTLAVRGAGVPDVEVHNRLFV
                     EGHSVGVVPGLLPDPLDRPASSVPAKSVRLGIERPRPDVRTYLCVEKTSWGGELAVNL
                     NLRAAMVSGDLFIECHAYVLLPLRAELTTVDHMPTGGAEIALRALRDAFGALRQLRRV
                     VKDERRESAVARKRRRALADDRRAIRKNRPFGRGAGLSIRAAAAADERIFLFAYTDEE
                     MYLNALQRRILGAIETFLDQHGIDTADFHQKLTNIINNHSYAIGAVNAQNAQVGNNNQ
                     MSGPPTQQPPPPPWSSTP"
     gene            1261..1584
                     /locus_tag="VFC01_12615"
     CDS             1261..1584
                     /locus_tag="VFC01_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80513.1"
                     /translation="MSTFNIGAVNADNALFGDGTVQINIQDLMQRLDADVARHRSRFP
                     EPATADRALADLRRECAAQPVDVDRVRAAAGALTAATAGAPGVHDGIEELLDRVTRMG
                     TGRHP"
     gene            2102..>2710
                     /locus_tag="VFC01_12620"
     CDS             2102..>2710
                     /locus_tag="VFC01_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN80514.1"
                     /translation="MPEHGILLPFYLVIDVSFSMSGAKLSTANAILPSVADALAKNPI
                     LNDKVRFSLVDFSDDARVVLPLCDVSTATQLPNLAVRGGTCYGAAFKLLRRQMQSDVA
                     QLRADGYKVHRPAVFFLSDGEPGDNWKDDFETLTEYDPSTGKGFKWHPVVVPCGVEDA
                     DRDVMRQLVHPPNKSKLYMMKDGSDASAAIKAMAEVLISSVLA"
BASE COUNT          461 a          911 c          893 g          445 t
ORIGIN      
        1 gcggctgcgc ctgtacctca cgggagcgat ggccgtgtcg acgcttggtc tcctcgccgc
       61 cgccgtgacc ggactcgcgg aactcctcct actcttcctg acgtgtggcg ggtgcggcat
      121 cttcctcgcc gtcttcttcg accggctctc gttgtaccgg gtcctcgcgg cgatggtgtc
      181 gccccaggtg ccgatgcgag acctggcccc ggccctgccc gcgcacatcg aggctcggct
      241 ggaggcgttg aacaagagca acgtcgtcat cttcgaggga ggggacgcgt tcatcgcttg
      301 gggtcggcgg ctgcgcgacg gatggcagat gaacatcgac gccaccagag cagccaccga
      361 caccaacggc aaggcacgta cggtcatccc gttcacgccg gtcgacctgc acaaggccgt
      421 gacgttggcg gtacgcggtg ccggtgtccc cgatgtagag gtgcacaacc ggctcttcgt
      481 ggagggccat tcggtcggtg tggttcccgg gctgcttccc gatccgttgg atcgccctgc
      541 ctcttcggtg cccgccaagt cggtgcgact cggcatcgag cggccacggc cggatgttcg
      601 gacgtacctg tgcgtggaga agacctcctg gggcggcgaa ctggccgtca acctcaacct
      661 gcgggccgca atggtaagcg gcgacctgtt catcgagtgc catgcctatg tccttctccc
      721 gctgcgcgcg gagctgacga cggtcgatca catgccgacg ggcggcgccg agatcgccct
      781 ccgggcactg cgtgacgcgt tcggtgcgct gcggcaactg cgtcgcgtgg tgaaagacga
      841 gcgacgggag tcggcggtgg cccgcaaacg tcgccgggcg ctcgcggacg atcgccgtgc
      901 catccgcaag aaccgtccgt tcggccgcgg agccgggctc agcatcaggg cggcggcggc
      961 cgcggacgaa cggatcttcc tcttcgcgta caccgacgag gagatgtatc tcaatgcctt
     1021 gcagcgacgg attctcggcg ccatcgagac gttccttgat cagcacggca tcgacacggc
     1081 ggacttccac cagaagctaa ccaacatcat caacaaccac agctacgcga tcggcgcagt
     1141 caacgcacag aacgcccagg tgggcaacaa caaccagatg tccgggccgc cgacgcaaca
     1201 gccaccgcct ccgccatggt cgtcgacacc gtaacaacta tcgccttggg agtcgaagca
     1261 gtgagcactt tcaacatcgg agcggtcaac gccgacaacg cgctgtttgg cgacggcacc
     1321 gtgcagatca acatccagga cctgatgcag cggctggacg ccgacgttgc gcggcaccgc
     1381 tcgcggtttc cagagccggc caccgccgac cgggccctgg ccgatctgcg tcgggaatgc
     1441 gccgcccaac cggtcgacgt tgaccgcgta cgggcggccg ccggggcgct gacagccgcc
     1501 accgctggcg ctcccggcgt ccatgacggc atcgaggagc tgctcgaccg cgtcacccgt
     1561 atgggcaccg ggcgacaccc gtgacggcgg tcgtctccgc cgatagcctg gcggtgaaac
     1621 catattgcac acgtcaggac cggttggttc gcctcacgct gcgggcccaa caggagatcc
     1681 tcgcggcggg tggtacgcag ctcggccgca ggccagcgct cgctaccgtc gccttcatgg
     1741 ccatcgcgat cgcggtttcc gacgtcgggt tctaccacca cgaggccaac cccggcatgg
     1801 tcgggatcgc cggcgccgca acgctgttca cgctgctcac aagttccgtg gcgatcatgc
     1861 ggcgggagct gcgcatgcgc cgggaccggc tgggggcggt cgaggacgac catcaaaagc
     1921 ctcgtcgcgt ccgtcgtgtt ccatcgcaac atgtcggacg tgcagcgggc gcggctcgtc
     1981 gacacggcgt tcgcccggga gctgccggcg gccgtgccga ggcaggctcc gcggaagaag
     2041 tcagtgaaag tcccacaccc cacggcgcag agtcgccgcg gcgccgaacg aggagagtga
     2101 catgcccgaa cacggcatac tacttccctt ctatctggtc atcgacgtca gtttttcgat
     2161 gtcgggtgcc aagttgagca cagccaacgc gatcctgccc tcggtcgcgg acgcgttggc
     2221 gaagaacccg atcctcaacg acaaggtccg cttcagcctg gtggacttct ccgacgacgc
     2281 ccgggtggtg ctgccgctgt gcgacgtgtc gacggcgacg cagctgccta acctcgcggt
     2341 ccgtggtggc acgtgctacg gcgcagcctt caagctgctg cgtcggcaga tgcaaagcga
     2401 cgtggcgcag ctgcgcgccg acggttacaa ggtgcaccgg cctgccgtgt tcttcctcag
     2461 cgacggcgag cccggtgaca actggaagga cgacttcgaa accttgacgg agtacgaccc
     2521 gtccaccggg aaaggcttca agtggcaccc ggtcgtcgtt ccatgtggcg tcgaggacgc
     2581 cgatcgcgac gtgatgcgcc agctggtgca tccgccgaac aagtcgaagc tgtacatgat
     2641 gaaggacggt agcgacgcat ccgccgcgat caaggcaatg gcggaggtgc tgatttcgtc
     2701 ggtactcgcg
//
LOCUS       DASRJA010000288         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1337281,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000288 DASRJA010000000
VERSION     DASRJA010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337281"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(892..1953)
                     /locus_tag="VFC01_12625"
     CDS             complement(892..1953)
                     /locus_tag="VFC01_12625"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZN80515.1"
                     /translation="MTLTRVHEKGISMTVIIGVDPHKSTHTAVAIDRDEQPLARVQLV
                     ADRCQVQRLLAWVEPLGTQRTWAVESAAGLGKLLAQQLLASGEQVVDVPPTLAARVRL
                     LGSTKAAKNDPNDALATAIAGLRHCGLRAVRVEDDTAVIRMVIDRYDDLVALRTQAAC
                     RLHVALRELIAGGAPRRLGAERAGKLLRSVRPQGAAGVERKRLALELLADVRRLDRDV
                     ATAKRRVSDAVTASGTSLLELHGVGPIVAGFILGHVGDPARFPTPARFASYNGTAPIE
                     ASSGPRSRHRLNPRGNRKLNHAFHLAAVTQVRHDTPGRVYYQRKIAEGKSKKEALRAL
                     KRRISDAVWRQLQVDIGRR"
     gene            2193..2600
                     /locus_tag="VFC01_12630"
     CDS             2193..2600
                     /locus_tag="VFC01_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80516.1"
                     /translation="MIHIRAVSPPDTTPRLVASLERNSGVLNLIVLQGVVRNPDGDAV
                     QFDVINAQANEVVLELRTLGVDRIGSIVIEDVGTELSDRAASVPWSSAPSTRPSSASR
                     SGSTRTIADGSAPARGPSSSAFSSQLLRRSSSA"
BASE COUNT          463 a          969 c          963 g          468 t
ORIGIN      
        1 gccttgcgta cattctgagc cgctcatgac cacgctcgtg tgacgctgct ccggccgggc
       61 gtctgcgggt gccggcaagt tccagcggtc ggggtggcca agtcacgcgc tgccctggca
      121 cacatgccgg gcgcacgaaa cgcggtcgca taccgttcac cagcgagcca cgttgatccc
      181 gtgcggaccg gcgacgccgc gagacgtcgg gcacgccgtc gtcgtcggca cacagatcga
      241 cggctccgct cccgccgcgc gcgccagaac aacggtggca tcgcacatct gatccgcgag
      301 cggagcgccg attcagttgc gtcttatgac ccggcaacgt gtaccgacag ctcattgtcc
      361 gcggacggca tcttgcgcca gacctatcaa taaccgaccc cacccccggc ggcgccaccg
      421 cccatggtga gagcgaggat caccacagcc gcgacgacga ctgcgacaga gacccagagg
      481 atgggagcac gaagaactcg ttgcatgatg gtcgtccttg tcttgagagt tggcaatcca
      541 gccggatcac cgtttcaccc ctcgtacggg cgcctagcga gattggttcg cgatccggcc
      601 taccggtgtg catgggtcgc cacgcggatc atcggaaccg agcggcccca gatgcccagt
      661 gaggccggcg ggcacggacc gcccgccgtt ccgcggcacc gaacccgtag cgccgatcga
      721 agcctctttg tgtcaagggg ccgtttcggg gcgcgtcggg gcagcgcagt gggcgcgggc
      781 gtagcgtggt ggttgggttc gggagtgact gttccgaagt gccggtgttc agggttcagc
      841 cggccacgct ggattgaaga gtcgtccctg gttgtcctcc cggacccgtt gtcagcggcg
      901 gccgatatcg acttggagct ggcgccagac ggcgtcgctg atgcgacgct tcagagcgcg
      961 cagcgcctcc ttcttcgact tcccctcggc gatcttgcgc tggtagtaga cccggcccgg
     1021 tgtgtcgtgg cggacctggg tgaccgcggc caggtgaaag gcatggttca gcttgcggtt
     1081 gccgcggggg ttgagccggt gacggctgcg gggcccgctg gaggcttcga tgggggcggt
     1141 gccgttgtag gacgcgaacc gcgccggggt cgggaaccgg gccgggtcac cgacgtgacc
     1201 caggatgaac cccgcgacga tcgggccgac accgtgcagc tcgagcagcg acgtgcccga
     1261 cgcggtgacg gcgtcgctga cgcggcgctt ggcggtggcg acgtcgcggt cgagacgacg
     1321 gacatcggcc agcagctcga gggcgagccg cttgcgttcg acccccgcgg caccctgcgg
     1381 gcggacgctg cgcagcagct tcccggcccg ttccgcaccg agacggcgag gcgcgccgcc
     1441 ggcgatgagc tctcgcagcg ccacatgcag acgacaggcc gcctgggtgc gcaatgcgac
     1501 cagatcgtcg tagcgatcga tcaccatccg gatcaccgcg gtatcgtcct ccacccgcac
     1561 cgcccggagc ccgcagtgcc gcaacccggc gatggccgtg gccaacgcgt cgttggggtc
     1621 gttcttggcc gccttggtcg agcccagcag ccgcaccctc gcggccaacg tcggaggcac
     1681 gtcgacgacc tgctcaccgg atgcgaggag ctgctgggcc agcaatttgc ccagccccgc
     1741 agctgattcg accgcccagg tccgttgcgt gcccaacggt tccacccacg ccaacagccg
     1801 ctgcacctga cagcggtcag ccaccagttg cacccgggcg agcggctgct cgtcccggtc
     1861 gatcgccacc gccgtgtgag tcgacttgtg cgggtcaacg ccgatgatca ccgtcataga
     1921 aatccccttc tcgtgaacgc gtgtgagcgt cacgacgggc actcctgatg aaggtgctgc
     1981 tcgcctcttt cgagccacgc cgtgaccgaa ccctggccgg cgagcgcttc gttcggaagc
     2041 caacccgaca cacgagcagc aggcagttcg agagccagcc gaccaagacc cgaggacgct
     2101 acgaacacac gctcaacgcc cagccatatc gaaccatcag gcagctctgt gcgggtcttc
     2161 gcagctcgcg acgcgagcgc gaacctggcg ggatgatcca catccgggcg gtgagcccac
     2221 cggacaccac gccgaggctg gtcgcgtcgt tagagcgcaa ctccggtgtg ttgaacctga
     2281 tcgtcctcca gggtgtggtg cgaaaccccg atggggacgc cgtgcagttc gacgtcatca
     2341 acgcgcaggc caacgaagtc gtgctcgagt tgcgcacact cggtgtggat cgcatcgggt
     2401 cgatcgtcat cgaagacgtc ggcacagagc tgtccgaccg ggcggcttcg gtgccatggt
     2461 cgtcggcccc gagtacgcgg ccatcatcag cgtcgcgctc gggatcaaca cgaacgatcg
     2521 cggacgggtc cgctccggct cgggggccct cttcgtcggc tttctcctcg caattgttgc
     2581 gacgttcgtc ttcggcctag tagtccgcgc ctttggttgg gaaccggagg cgttcagcct
     2641 tggcgtccgg ccggtatcgg acctgaagca cgcggctcgc tgactcagct gctgctgaac
     2701 gtggcgatct tgatcgcggt gggcgcagca ggcctcgtcg tgcagcagcg tctctggcgg
     2761 cgcgtcggca gaagagcaac caaagatagc ccgcaggctt agacggtcat ccgtgcacgt
     2821 cgctgacctc gcccgacgag cgacggtctc ttcggcgtga tca
//
LOCUS       DASRJA010000289        17825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_314197,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000289 DASRJA010000000
VERSION     DASRJA010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17825
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314197"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VFC01_12635"
     CDS             <1..978
                     /locus_tag="VFC01_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN80517.1"
                     /translation="RYLLVGLLLLGFTAACNDLASVPYNAMLRQLTTPETYGKVSGFG
                     WAAGYIGSMVLLIVVYAGFIKGGGPTTGLLDIPTADGQNVRAAMLVTAAWMAVLALPV
                     LLAPPPVDVEQPAPSSTGVLGAYRKLWADLVGEWRRDRNVVYYLLASAVFRDGLGGVF
                     TFGAVLGVTVYGISAADVLLFGITANVVAAAGAALGGLFDDRVGSKAVILSSLAAMIA
                     VGLTLMALSGAAAFWVCGLLLCLFIGPTQASARTLLLRMTAEGKEGVAFGLYTMTGRA
                     VAPLAPFMFSTFVAIFGAARAGIGGLMLVLAAGFVAMLAVRAPRRAAAG"
     gene            complement(975..1727)
                     /locus_tag="VFC01_12640"
     CDS             complement(975..1727)
                     /locus_tag="VFC01_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HZN80518.1"
                     /translation="MTESGRRDGPEPFGNSYPGYSDPAYASQAPYGPTYQAPAAPAPT
                     ERLPYPPAGYDPYATGQYGPQYPPGEPPQPPDGPKSPRWLWIVAALAVVTVIGLVIAL
                     VVVNSSKQDVIVAPPPTLSEPSSTRPTPTTTPRPTIPVFPLPTLPSTPSTTGPSSPST
                     PGATETVVYDVSGDGRAINITYVDTGGVLQTEFNVMLPWSKEVELAEPAEDSASVSII
                     NVGREVSCSISVNGVQVQQRTGAGLTICTAAG"
     gene            1879..4503
                     /locus_tag="VFC01_12645"
     CDS             1879..4503
                     /locus_tag="VFC01_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase/circularly permuted
                     type 2 ATP-grasp protein"
                     /protein_id="HZN80519.1"
                     /translation="MRGVMPRTMGVEEEFHLVDQKTRRLTARAPELLGVLSDSYVAEL
                     QRCVVEMNSGVVDTLDGLRADLQNHRKVLVDAAAKLGMGVVAAGAVPLSVPAEMQVTQ
                     TARYRQMLADYQLLAREQLICGTQVHVGVADRDESVVVANRVSAYVPTLLALSASSPF
                     WSDGSDTGYSSVRTLVWQRWPTTGLAAPVSSAAEYDKLVAELVASGAITDDGMVYFDV
                     RPAVAAPTLELRVCDSCPSVDTIVLIAGLFRALVERELEGFRAGVPAVEVSPPLGRAA
                     LWRAARSGLEGDLVDIDGPVSRPAGDVVTELIRSLRPQLEAAGDWQMIVELTRQVLIA
                     GTSAARQRRALRRRGRLTDVVDQLIAETAGSWPDTATAVIEDPTLLFGYQPDREYDPA
                     DKAAAVSYDEAVDPTGRARPRYEKILHAVADLGVAVLRSREGDIEQDQRAESITFRVS
                     GQNRAQVFPLDLMPRLVPADEWAVLTAGLGQRAKALNAFLRDIYSEQAILADGVIGMH
                     TLDRAPGFRSTGRLSRDPVRAHVSGTDLVCDKAGNWMVLEDNLRIPSGTAYAIANRRL
                     LTKHLPELELPAELGDVDQVPAMLLETLRAAAPPAAGDEPSVALLSAGWEDSAWFEHT
                     FLAEELGIPLVQTSDLSVRDGKLFRHIGSDVHPVDVLYARMDEDMLLSSAGYDASALR
                     PGLLEAVTNGTLTIANALGNGVADDKAVYAYVPAMINYYLGEKPALAQVPTWICAERA
                     QRDYVLDNIAELVVKPIDGHGGTGVVIGPEAAADALEVRRRELQSQPERYIAQQAIAL
                     STHPTFDGEGMYPHHVDLRAFVHLRPGFDDTVTAHVMPAGLTRVAARGSRIVNSSSGG
                     GSKDTWILTGGQHDQAAP"
     gene            4517..6301
                     /locus_tag="VFC01_12650"
     CDS             4517..6301
                     /locus_tag="VFC01_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HZN80520.1"
                     /translation="MCGICGEIRFDGQSADVAAVTRMTAAMASRGPDSDGVVAHGPLA
                     FGHRRLSIIDLSARGEQPMVDSELGLTLVFNGCIYNYQDLRKELEAVGYQFFSAADSE
                     VVIKAFHRWGTGCVDRFKGMFAFAIADRETGVVTLARDRLGIKPLYLAETPGRLRFAS
                     TVRALLDAGEVDTELDRYALHHYMSFHSVVPAPRTIYRGVRKMPPATVRVIQPDGAHV
                     DTVYWTPLFERDPARASWTERDWQEALMDALRAAVERRMVADVPVGVLLSGGIDSSIV
                     VALLAEQGQHGLATFSIGFDSAGDESGDEYVYSDLIATTFDTDHHRIHIDGSRLLPAV
                     PKTIAAMSEPMVSHDCVAFYLLSQEVSKSVKVVQSGQGADEILAGYDWYPPLANVARD
                     QTSEAYAKVFFDRGHSDVLAIFAPEYAIVEEDPSRQFVVTHQSARGAETAVDAALRLD
                     TQVMLIDDPVKRVDTMTMAWGLEARVPFLDHDFVELAASCPAELKLASGGKGVLKGAS
                     RGLLPAEVIDRTKGYFPVPGIRHLHGDMLDMVRDALTNDAARTRGLYREETVDALLAA
                     PNKTRTTLGANALWQLAVLEMWLQSMEN"
     gene            6306..8183
                     /locus_tag="VFC01_12655"
     CDS             6306..8183
                     /locus_tag="VFC01_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN80521.1"
                     /translation="MGQQVRANYGASLSPDATAFAQLVDDGGFPRAVQRFLRGWRASS
                     SREVELPVEGAVTRVLHSADGHWLACEVAPDGATRSQIWVVTTDPDDRDARRIDYWPA
                     GAQEGTAELIAWDGTQVAAILTGEDGVGSSCLIDPSDGTTVVLDRRSGGRLVDAWAGA
                     SLVRVGPRGYRDLIMLRGLTEIALLPYDPGSTTDAGIILDDHHPRRLRAGLEGERTEL
                     YQPAKLYGINSPEGYLRALIRSENGADHARLLEVTSTPDGVSYQVLAERPGYELDEFT
                     VSDDVSTVALLWNINGTSELQILELPDNTLHDPIPLPGMVASELSISAGGSMVAMTVE
                     GPSLPPTVELVDPRTREWEPVDREPSSGPVAADPTLETITARDGLEFTGWLFRPPPGV
                     EPVGAMLFLHGGPEGQGRPGYNEFFPPLLEAGITVFLPNVRGSGGFGRTFMHADDKER
                     RFAAIDDVADAVRYLVDFDLAPRDRVACTGWSYGGYLTQAALTFHPQLFAAGISICGM
                     SDLNTWYRNTEPWIAAAAHPKYGHPVSDRDLLDRLSPLLRAHAMTAPLLLVHGLNDTN
                     VPPTESEQMYDALRVLGRRVELLVFDDDGHEIVKRENRAVLVKAMKDWLTTAFAATSI
                     S"
     gene            8281..8490
                     /locus_tag="VFC01_12660"
     CDS             8281..8490
                     /locus_tag="VFC01_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80522.1"
                     /translation="MRGGGILGVIVLVWLLIGVLAAYQRDYFKSGENNCATAGSIALT
                     VVVGPLNYAGVNPKVANCNLPEPSQ"
     gene            8532..8687
                     /locus_tag="VFC01_12665"
     CDS             8532..8687
                     /locus_tag="VFC01_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6131 family protein"
                     /protein_id="HZN80523.1"
                     /translation="MIVLGAILLILGLIFGISILTYIGVVLIVVGAIFWILGSMGRPV
                     GGRRAWY"
     gene            complement(8694..9317)
                     /locus_tag="VFC01_12670"
     CDS             complement(8694..9317)
                     /locus_tag="VFC01_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80524.1"
                     /translation="MATSTPATTRRSRAKSDRRSQLITAAERLVAERGYLAVRLEDIG
                     AAAGVSGPAIYRHFPNKEALLVELLVGISTRLLAGATDVVAQAGDPEQALDGLIEFHL
                     DFALGESDLIRIQDRDLGHLPPTAKRQVRKAQRQYVEIWVNVLRQLDHALSEADARLM
                     AHATFGLLNSTPHSVKPGGTKTAEASSRAVLRAMTVAALTSAGRAER"
     gene            9414..10964
                     /locus_tag="VFC01_12675"
     CDS             9414..10964
                     /locus_tag="VFC01_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZN80525.1"
                     /translation="MTTRVSHREEHLALVEKLRAKLATGALGGPERARERHVSRGKLL
                     PRDRVDGLLDPGSPFLELAPLAADGMYDDECPGAGMIAGVGRVSGRECMIVANDATVK
                     GGTYYPITVKKHLRAQEIAGQNRLPCIYLVDSGGAFLPRQDEVFPDREHFGRIFYNQA
                     NLSAQGIPQIAAVLGSCTAGGAYVPAMSDEAVIVRNQGTIFLGGPPLVKAATGEVVTA
                     EELGGGDLHSKTSGVTDHLAHDDRDALRIVRRIVATLGPRTEVPWDVAPTVDPIAEGA
                     ELYDVVPVDPRVPYDVHEVITRIVDGGEFAEFKADYGSTLVTGFARIHGHPVGIVANN
                     GVLFSESALKGAHFIELCDKRMTPLLFLQNISGFMVGRDYEAGGIAKHGAKMVTAVAC
                     ARVPKLTVVIGGSYGAGNYSMCGRAYSPRFLWMWPNARISVMGGEQAASVLATVRGEM
                     TADEEEKFKAPIRQQYEDQGNPYYSTARLWDDGVIDPADTRTVLGLALSIVGQAPLES
                     VSYGVFRM"
     gene            11068..13059
                     /locus_tag="VFC01_12680"
     CDS             11068..13059
                     /locus_tag="VFC01_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HZN80526.1"
                     /translation="MTTFDTVLIANRGEIAVRVTRTLRAMGIRSVAVFSDADAGARHV
                     AEADVAVGIGPAPARQSYLNIDAVVAAAKRTGAQAVHPGYGFLSENAQFAAALQAAGI
                     VFIGPPAAAIQTMGDKIAAKAAVSAFGVPVVPGVSRPGLTDADLIAGADEVGYPVLIK
                     PSAGGGGKGMRVVHEPSELAVALASARREAASAFGDDTLFLERFVLNPRHIEVQVLAD
                     GYGNVVHLGERECSLQRRHQKVIEEAPSPLLDKATRARIGAAACDTARSVDYTGAGTV
                     EFIVSADRPDEFFFMEMNTRLQVEHPVTEMVTGIDLVEQQVRIAAGEKLPIEQDDITM
                     TGHAIEARVYAEDPARGFLPTGGKVLDLAEPEGPGVRVDSGLAAGMLIGSEYDPMLAK
                     VIAHAGDRPAALRALDRALADTAVLGVTTNIEFLRFLIADPDVAAGRLDTGLLDRRTP
                     DYVPAQHGDDELIAAAAYKWLRGWPTPVGDLWEVPSGWRIGERAPTTFRLHAAERTDH
                     VYLTGTPQAATARVEHGGSHSLTASLDDDRLAVTLDGLRTDYLVAATDHQIWLSGGGR
                     TAMVEEMREAPVRPDDEHSGDAELTSPMPGSVVAVGVEDGQSVDAGTVVVTVEAMKME
                     HALSAPVDGAVELLVAVGDQVKVGQPLARITASTKEDES"
     gene            13056..14216
                     /locus_tag="VFC01_12685"
     CDS             13056..14216
                     /locus_tag="VFC01_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80527.1"
                     /translation="MSDFLATGTLPDHYEQLAKTVRDFAQSVVAPVSAKHDEEHSFPY
                     EVVSGMADMGLFGLPFPEEYGGMGGVYFALCLALEELGKVDQSVAITLEAGVSLGAMP
                     VYRFGNEEQKQEWLPLLASGKALGAFGLTEAGGGTDAGATRTTAKLDDGHWVINGSKQ
                     FITNSGTDITKLVTVTAVTGDTGGRKEISSILVPVPTEGFTAEPAYNKVGWNASDTHP
                     LSFDDVRVPAENLLGERGRGYANFLRILDEGRIAIAALSVGAAQGCVDECVKYAKERQ
                     AFGAAIGTYQAVAFKIARMEARAHAARTAYYDAAALMLAGKPFKKAAAIAKLVASEAA
                     MDNSRDATQVFGGYGFMNEYAVARHYRDSKILEIGEGTTEVQLMLIAREAGL"
     gene            14213..14695
                     /locus_tag="VFC01_12690"
     CDS             14213..14695
                     /locus_tag="VFC01_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZN80528.1"
                     /translation="MTDKKVVVQRGLWFEEFETGVLYQHRPGRTITEADNVLFTTLTM
                     NTQALHLDAAFSDALPPFNQRLVNSMFTLSTLVGLSVAQLTQGTIVGNLGFGEVAFPK
                     PLFHGDTLYAESEVTDKRESKSRPGEGIVTFAHTGRNQHGDIVATASRKTMVRKKPEE
                     "
     gene            14695..15516
                     /locus_tag="VFC01_12695"
     CDS             14695..15516
                     /locus_tag="VFC01_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZN80529.1"
                     /translation="MALTQTGPAWLFCPADRPERFEKAAAAADVVILDLEDGVAAKDR
                     EAARRALIETPLDPDRTVVRINPSATADHQLDLEALKRTSYTTVMLAKTESVQQVSAL
                     LPLDVVVLIETPLGALHVVELSRLGNAIALMWGAEDLFAVLGGTANRHVDGTYRDVAR
                     HVRSQTLLAAKAYGCMALDSVFIDIKDLDGLRDEVDDAVAVGFDAKVAIHPSQVAVIR
                     AGYAPTAEQVEWARHVLAAARDERGVFQYEGLMVDAPVLRRAERIVALAPNDRGW"
     gene            15528..15774
                     /locus_tag="VFC01_12700"
                     /pseudo
     CDS             15528..15774
                     /locus_tag="VFC01_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003404760.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            complement(15918..17156)
                     /gene="glgC"
                     /locus_tag="VFC01_12705"
     CDS             complement(15918..17156)
                     /gene="glgC"
                     /locus_tag="VFC01_12705"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZN80530.1"
                     /translation="MADPKVLGIVLAGGEGKRLSPLTLDRAKPAVPFGGLYRLIDFAL
                     SNLVNAGVLRIAVLTQYKSHSLDRHITTTWRMSSLLANYVTPVPAQQRLGPQWYTGSA
                     DAIYQSLNLIHDEKPDLVIVFGADHVYRMDPRQMIAQHLDSGAGATVAGIPVPREEAS
                     AFGVIKTGSDGLRVEAFLEKPPDPPGLPGRPDETFASMGNYLFSADALVEVLRKDADD
                     ESSKHDMGGNIMPMMVDRGMAQVYDFQRNEVPGADPADAGYWRDVGTLDSYYDAHMDL
                     CAVVPVFNLYNSEWPILTHIPPHPPAKFVHEDGERVGHAINSVVSNGVIVSGALVRES
                     VLSPGARIEDYAIVERSVIMDNTVVAANAVVQNAIIDKDVVVPEGAQVGVDKDHDRAR
                     GFVVSDGGVTVVGKSQEVTK"
BASE COUNT         2974 a         5794 c         5967 g         3090 t
ORIGIN      
        1 cgctacctgt tggtcggctt gctcctgctc gggttcaccg cggcgtgcaa cgacttggcc
       61 agcgtcccgt acaacgcgat gctgcgacag ctgacgacgc cggagactta cggaaaggta
      121 tccggattcg gttgggccgc aggctatatc ggcagcatgg tgctgctcat cgtggtctat
      181 gcgggattca tcaagggtgg cggcccgacg accgggctgc tggatatccc gaccgctgac
      241 gggcagaacg ttcgggcggc aatgctggtt accgcggcct ggatggccgt gttggcgctg
      301 ccggtcctcc tcgcacctcc gcccgtcgac gtcgagcagc cggcgccgtc gtcgaccggt
      361 gtgctcggcg cctaccgcaa gctgtgggcg gacctggtcg gcgagtggcg gcgagaccgc
      421 aacgtcgtct actacctgct cgccagcgca gtgttccggg acggattggg cggtgtgttc
      481 accttcggcg cggtgctagg cgtgaccgtg tacggcatct cggcggccga tgtgctgctc
      541 ttcggtatca ccgccaacgt ggtggccgcg gccggggcgg cactcggcgg gctgttcgac
      601 gaccgcgtcg gctcgaaagc cgtcatcttg agttcgcttg cggcgatgat cgccgtgggc
      661 ttgaccctga tggcgttgtc cggtgcagcg gcgttctggg tgtgcggact tctcctgtgc
      721 ctgttcattg ggccgacgca agcatcggcg cgcaccctgc tgctgcggat gaccgccgag
      781 ggcaaggagg gggtggcgtt cggtctctac accatgacgg gccgggccgt ggcccccctc
      841 gcgcccttca tgttctccac tttcgttgcc atcttcgggg cggctcgcgc gggcatcggc
      901 ggtctaatgc tggtgttggc ggcaggtttc gtcgcgatgc ttgcggtgcg ggcgccgcgt
      961 cgtgcggcgg ccggctaacc cgccgcggtg cagatcgtca gccctgcgcc agtgcgctgc
     1021 tggacctgca caccgttgac gctgatcgag cagctcactt cgcggccgac attgatgatg
     1081 ctgacgctcg cggagtcctc ggccggctcg gccagctcga cctccttgct ccacggcagc
     1141 atcacgttga actcggtctg caacacacca ccggtatcga cgtaggtgat gttgatggcc
     1201 cgaccgtcac ccgacacgtc atacacgacc gtttcggtcg cgcccggcgt cgatgggctg
     1261 gacgggcccg tggtggacgg cgtgctcggc agcgtcggca gcgggaagac cgggatcgtc
     1321 ggacgtggcg tcgtcgtcgg tgtcggcctg gtggagctcg gctcggacaa cgtcggcggc
     1381 ggtgcgacga tgacgtcctg cttcgagctg ttgacgacca ccagcgcgat caccagcccg
     1441 atgacggtca cgaccgcgag ggccgccacg atccacagcc accgcggcga cttcgggccg
     1501 tccggcggct gcggaggttc gcccggcggg tactgcgggc cgtactggcc ggtcgcatag
     1561 gggtcatagc cagcgggcgg gtagggcagc cgctcggttg gcgccggcgc ggccggtgcc
     1621 tggtaggtgg gtccgtaggg tgcctggctc gcatacgccg gatcggagta tccgggatag
     1681 ctgttaccaa agggttcggg cccgtcgcgg cgtcctgact ccgtcatacc gacctcatgg
     1741 ctgcagggta cctgcgtaat tcgttgaacg gcttcttcgt cgcggcggcc ctattctggc
     1801 gaaagcgcta gccgcagcgt ttaccggcaa cgaccgcggt taacgccgac gtaactacgc
     1861 gtcaattacc gtggcgaaat gcgaggcgtc atgccgcgaa cgatgggcgt cgaggaggaa
     1921 ttccacctcg tcgaccagaa gaccagacgg ctgacggcgc gcgcgccgga gctcttgggc
     1981 gtgttgtccg acagctacgt cgccgagttg cagcgctgcg tggtcgaaat gaacagcggc
     2041 gtggtcgaca cgctcgacgg actgcgcgcc gatctgcaga accaccgcaa ggtgcttgtc
     2101 gacgccgccg ccaaactcgg catgggcgtg gtcgccgctg gggcggtgcc gctgtcggtt
     2161 cccgccgaaa tgcaggtcac gcagactgcc cgctaccgcc agatgctcgc cgattaccag
     2221 ctactggctc gcgaacaact catctgcggc acccaggtgc acgtcggcgt ggccgaccgc
     2281 gacgagtcgg tcgtcgtggc caaccgagtg tctgcgtatg tgccaacact tttggcgctg
     2341 tccgccagct cgccattctg gtccgacggc tccgacaccg gctactccag cgtgcgaacc
     2401 ttggtgtggc agcgttggcc gacgacgggt ctggccgcgc cggtgtcctc agccgccgaa
     2461 tacgacaaac ttgtggccga gctggtggca agcggagcaa tcacggacga cgggatggtg
     2521 tacttcgacg tccgtccggc cgtcgccgcg cccacgctgg aactgcgggt gtgcgacagc
     2581 tgcccctcgg tggacaccat cgtgttgatc gcggggctgt tccgggcatt ggtcgaacgc
     2641 gagttggagg ggttccgggc gggtgtccct gccgtcgagg tttcgccgcc gctgggccgt
     2701 gcggcgctgt ggcgggcggc ccgctcgggt ctggagggcg atctggtcga catcgacggt
     2761 ccggtcagcc ggcccgccgg agacgttgtc accgaactca tacgctcgct gcgaccgcag
     2821 ctggaggcgg cgggggactg gcagatgatt gtggagctga cccggcaggt gctgatcgct
     2881 ggcacgtcgg cggcccgtca gcgacgggct ttgcgtcgcc gcggccggct aaccgatgtc
     2941 gtcgaccagc tgatcgccga aaccgcgggc agctggccgg acactgcgac ggccgtaatc
     3001 gaagacccca cactgctgtt cggctatcaa cccgaccgcg agtacgaccc agccgacaag
     3061 gccgccgccg tcagctacga cgaggccgtc gaccccactg ggcgggcgcg gccccgctac
     3121 gaaaagatcc tgcacgccgt cgccgacctc ggggtcgcgg tgttgcggtc ccgggagggc
     3181 gacatcgagc aggaccagcg cgccgagagc atcacgttca gggtcagcgg acaaaaccgc
     3241 gcccaggtgt tcccgctcga cctgatgccg aggttggtcc ccgccgacga gtgggcagta
     3301 ctcactgccg gactgggtca gcgggccaaa gcgctcaacg ccttcctgcg tgacatctac
     3361 tccgagcagg ccatcctcgc cgacggcgtc atcgggatgc acacgctgga ccgggcaccc
     3421 ggattccggt cgacgggccg gctgtcgcgc gaccccgtgc gcgcccacgt cagcggtacc
     3481 gacctggttt gcgacaaggc cggcaactgg atggtgctcg aagacaacct gcgcatcccg
     3541 tcgggcaccg cgtatgcgat cgcgaaccgg cggttgctca caaagcacct gcccgagctt
     3601 gagctgcccg ctgagctcgg cgacgtcgac caggtgccgg cgatgctgct ggagaccctg
     3661 cgtgccgcgg cgccgccggc ggccggcgac gaaccgtcgg tggcgctgtt gtccgctggc
     3721 tgggaagact ctgcctggtt cgagcacacc ttcctggccg aagagctggg catcccgctt
     3781 gtgcagacct cggacctgtc ggtccgcgac gggaaactgt tccgccacat cggttctgac
     3841 gttcaccctg tcgacgtgct gtacgcccgg atggacgagg acatgctcct gtcgtccgcc
     3901 ggatacgacg ccagcgcgtt gcggcccggt ctgctagagg cggtcaccaa cgggacgctg
     3961 accatcgcca acgcgctggg caacggcgtc gccgacgaca aggccgtcta cgcgtacgtg
     4021 ccggcgatga tcaattacta cctcggtgag aagcccgctc tggcgcaggt gccgacgtgg
     4081 atctgcgccg aacgcgcgca gcgtgattac gtgctggaca acatcgccga gttggtggtc
     4141 aaaccgatcg acgggcacgg cggtaccggc gtggtgatcg ggccggaggc cgccgccgac
     4201 gcgctggagg tccggcgtcg cgagctgcag agccaaccgg agcgctatat cgcccaacag
     4261 gcgatcgcgc tgtcgaccca cccgacattc gacggagagg gaatgtatcc gcaccacgtc
     4321 gacctgcggg cgttcgtgca cctgcggccc gggttcgacg atacggtgac ggcacacgtc
     4381 atgccggccg ggctgacccg ggtggcagca cgcggatccc gcatcgtgaa ctcatcctcg
     4441 ggcggcggca gcaaggacac ctggatcttg actggtggcc aacacgacca ggcggcgccg
     4501 tagaacggaa ggcaacatgt gcggcatctg cggagaaatc cgcttcgacg gacagtcggc
     4561 cgacgtggcc gcggtgaccc ggatgacggc tgccatggcg tcccgcggcc ccgactccga
     4621 cggcgtcgtc gcgcacggcc cgctcgcttt cgggcacagg cgcctgtcga tcatcgacct
     4681 gtccgcgcgg ggcgaacagc cgatggtcga cagcgagctc gggctgaccc tggtgttcaa
     4741 cggctgtatt tacaactacc aggatctgcg taaagagctc gaagctgttg gctaccaatt
     4801 cttttcggct gccgacagcg aggtcgtaat caaggcgttt caccggtggg gcacgggttg
     4861 cgtggaccgc ttcaagggca tgttcgcctt cgccatcgcg gaccgcgaaa ccggcgtcgt
     4921 cacattggcg cgcgaccggc tcggcatcaa gccgctctat ctggccgaaa cgcccggccg
     4981 gttgcgattc gcgtcaaccg tgcgcgctct actagacgcg ggcgaggtgg acaccgaact
     5041 cgaccgctat gcgctgcacc attatatgag ctttcattct gtggtgcccg cgccgcgcac
     5101 catctaccgc ggcgtgcgca agatgccacc ggcgaccgtg cgggtaatcc agcccgacgg
     5161 cgcacacgtc gacacggtct attggacgcc gcttttcgag cgcgatccgg cacgggcgtc
     5221 ctggacagag cgcgactggc aggaggcact gatggacgcg ctgcgcgcgg cggtggaacg
     5281 gcggatggtc gccgacgtcc ccgtcggcgt cctgctgtcc ggcggcatcg actcgtcgat
     5341 cgtggttgca ctgctggccg agcagggaca gcacggcctg gcgacctttt ccatcggctt
     5401 cgactcggcc ggtgacgagt ccggcgacga gtatgtctac tccgacctga ttgcaacgac
     5461 tttcgacacc gaccatcacc gcatccacat cgacggctcg cggctgctgc ccgcggtacc
     5521 gaaaaccatt gcagcgatga gtgaaccgat ggtcagtcac gactgcgtcg cgttctatct
     5581 cctgtcacag gaagtgagca agtcggtcaa ggtggtgcag tccggccagg gcgccgacga
     5641 gatcctggcc ggctacgact ggtacccgcc cctggccaac gtggcgcgcg accagacctc
     5701 cgaggcgtac gccaaggtgt tcttcgaccg cggccactcc gacgtgctgg caattttcgc
     5761 accggagtat gcgattgtcg aggaagaccc gagccgccag ttcgtcgtga cccaccagtc
     5821 cgcgcggggc gccgagacgg ccgtcgacgc cgcgctgcgg ttggacaccc aggtgatgct
     5881 catcgacgac cccgtcaagc gggtggacac catgacgatg gcgtggggcc tggaggcccg
     5941 ggtgccattc ctcgaccacg acttcgtcga gctggccgcc agctgtccgg ccgagctgaa
     6001 gctcgcatca ggcggcaagg gggtgctcaa gggcgcgtcg cgggggctgc tgcccgccga
     6061 ggtgatcgac cgcaccaagg ggtacttccc ggtgcccggc atccggcact tgcacggcga
     6121 catgctggac atggtccgcg acgcgctgac caacgacgcc gcccggaccc gcggcctgta
     6181 tcgcgaggag acagtcgacg cgctgctggc cgccccgaac aagacgcgta ccacgctcgg
     6241 cgccaacgcg ctctggcaac ttgcagtgct cgaaatgtgg ttgcagagta tggaaaatta
     6301 gtcccgtggg acagcaggtg cgcgcaaact atggcgcgtc gctgtcgccg gatgccaccg
     6361 cgttcgccca acttgtcgac gacggcggct tcccgcgtgc cgtgcagcga ttcctgcgcg
     6421 gttggcgggc gagttcgtcg cgcgaggttg aactgcctgt tgagggcgcc gtcacccgtg
     6481 tccttcattc cgccgacggc cactggttgg cctgtgaggt cgcacccgac ggcgccaccc
     6541 gcagccagat ctgggtcgtg acaaccgatc ccgacgaccg cgacgcgcgc cgcatcgact
     6601 attggcccgc aggcgcccag gaaggcaccg cagaactgat cgcgtgggac ggcacccagg
     6661 tcgccgccat cctcaccggt gaggatggcg tcggcagctc ctgtctgatc gatccgtccg
     6721 atggcaccac ggtggtgctc gaccgccgtt ccggtggcag gctcgtcgac gcgtgggcgg
     6781 gcgcgtcgtt ggtgcgggtc gggccgcgcg gctaccgcga cctgatcatg cttcgcggac
     6841 tcaccgaaat cgcgctgctg ccatacgatc ccggttccac caccgatgca gggatcatcc
     6901 tcgacgacca ccatccgcgc cggctgcgcg cagggctgga aggagaacgc accgagctgt
     6961 accagccggc gaagctctac gggatcaaca gccccgaggg ctacctgcgc gccctcatcc
     7021 gcagcgagaa cggcgccgac cacgcccgcc tattggaggt cacctcgacc cccgacgggg
     7081 tgtcgtatca ggtgctcgcc gagcggcccg ggtacgaact cgacgagttc accgtaagcg
     7141 acgacgtctc gacagtggcg ctgttgtgga acatcaacgg caccagtgaa ttacagatcc
     7201 tcgaactccc ggacaacacg ctgcacgacc caattccgtt gcccggcatg gtcgccagcg
     7261 agctcagcat cagcgccggt gggtcgatgg tcgcgatgac ggtcgaaggc ccatcgttgc
     7321 cgccgacggt cgaactggtt gatccccgca cccgggaatg ggagcccgtc gacagggagc
     7381 cgagcagcgg gcccgtcgct gccgatccca ccctcgagac catcacggcg cgcgacgggc
     7441 tggagttcac cggttggtta ttccggccgc cgcctggcgt cgaaccggtc ggcgcgatgc
     7501 ttttcctgca cggcggaccg gaagggcagg gccgtccggg ctataacgag ttcttcccgc
     7561 ccctgctgga agcgggcatc accgtattcc tgccgaatgt ccgtggttcc ggggggtttg
     7621 gccgaacgtt catgcacgcc gacgacaagg agcggcgatt cgcggcgatc gatgatgtcg
     7681 ccgacgcggt gcgctatctg gtcgactttg acctcgcccc cagagaccgc gtcgcatgca
     7741 ccggatggtc ttatggcggg tacttgacgc aggcggcgct cacgttccat ccgcaactat
     7801 tcgccgcggg gatcagcatc tgcggcatga gcgatctgaa cacgtggtat cgcaacaccg
     7861 aaccgtggat cgccgcggcc gcccacccga agtacggcca ccccgtcagc gatcgggacc
     7921 tgctcgaccg gttgtcaccg ctactgcgtg cacacgcaat gactgccccg ttgctgttgg
     7981 tccacggtct caatgacacc aacgtcccgc ccaccgaatc ggagcaaatg tacgacgcgt
     8041 tgcgtgttct cggccgccgc gtcgaactgc tggtcttcga cgacgacggc cacgagatcg
     8101 tcaaacgaga aaaccgagct gtgctggtca aggccatgaa ggactggctc accacggcgt
     8161 tcgcggccac ctctatttcg taaccaatcc gtagctgccg gaaggtttgg acaaattgtc
     8221 cgccgggtaa accggacctg cgcgtcagtc tctggcgcaa tacacggatg gaggcacagc
     8281 atgagaggtg gcggaattct cggtgtcatt gtgctcgtct ggttgctgat cggggtgcta
     8341 gccgcctacc agcgcgatta cttcaaaagc ggcgaaaaca attgcgcgac agcagggtcc
     8401 atcgccctca cggtggtcgt aggtcccttg aattacgcag gcgtcaaccc caaggtggcc
     8461 aactgcaacc tgcccgaacc gagccagtag tcaactggca gttattaatt gatgaagaaa
     8521 tggagtaaac catgattgtc ctcggagcca ttctgctcat ccttgggctg atctttggca
     8581 tatcgatcct gacctacatc ggcgtagtgc tgatcgtggt cggcgccatc ttctggatcc
     8641 tcggatctat gggacggccc gtcggcggca ggagagcttg gtactaacgc tcgctaacgc
     8701 tcggcacgac cggccgaggt cagcgctgcg accgtcatag cccgtaggac ggcgcgcgaa
     8761 ctggcctcgg ccgttttcgt gccaccgggt ttcacgctgt gtggtgtcga attcaacaac
     8821 ccgaacgtcg cgtgcgccat caggcgtgcg tcggcttcgc tgagggcgtg atccaactga
     8881 cgcaacacat tcacccaaat ctctacgtat tgccgctgcg ccttacgcac ctgccgcttg
     8941 gccgtcggtg gcagatgacc aaggtcgcga tcctggatgc ggatcaagtc cgactcgccc
     9001 agcgcgaagt ccaggtgaaa ctcgataagt ccgtccagcg cttgttcagg atcgccggcc
     9061 tgtgcgacga cgtcggtagc gccggccagc agtcgcgtgc tgatcccgac cagcagctcg
     9121 acaagtagcg cctctttgtt ggggaagtgc cggtagatcg cgggtccgct gacgcccgcg
     9181 gcagcgccga tgtcttccag tcgcaccgcg agatatccgc gctcggcaac aaggcgttcg
     9241 gccgcggtga tcaactggct gcggcgatcc gatttcgccc ggctgcgccg ggtggtggcc
     9301 ggcgttgatg tcgccatctg acccttccgg ctcgctagtg gacacttgag ttaatcgcga
     9361 ctaacatacc atgagttagt cgtcattaac tcaaacgaac gggttggcct tcgatgacaa
     9421 cgcgggtctc acatcgcgag gagcacctcg ccctggtcga aaagttgcgc gcaaagctgg
     9481 ccaccggggc gctcggtggt ccggaacgcg cgcgcgagcg gcatgtcagt cgcggcaagc
     9541 tgctgccccg cgaccgcgtg gatggtctgc tcgaccctgg cagccccttc cttgaacttg
     9601 cgccattggc cgccgacggc atgtacgacg acgaatgccc cggcgccgga atgatcgcag
     9661 gagtcggccg agtatccggt cgcgaatgca tgatcgtggc caacgacgcg acggtgaagg
     9721 gcggcacgta ttacccgatt accgtcaaga agcacctgcg tgcgcaggag atcgccggac
     9781 aaaaccggct gccctgcatc tacctggtgg actcgggcgg cgcgttcctg ccccggcagg
     9841 acgaggtgtt ccccgaccgc gagcacttcg gtcgcatctt ctacaaccag gccaatctgt
     9901 cggcccaagg cattccacag atcgctgctg tgctcgggtc gtgcacagcc ggcggtgcgt
     9961 acgtgccggc gatgagcgat gaggccgtga tcgtgcgcaa ccagggcacc atcttcctcg
    10021 gcggcccacc gctggtgaaa gccgcgaccg gcgaggtggt gacggcagaa gagctcggcg
    10081 gcggcgacct gcactccaag acttccggtg tcacagacca tttggcgcac gacgaccgcg
    10141 acgcactgcg gatcgtccgt cgcatagtcg ccacattggg accacgcaca gaggtgccgt
    10201 gggacgtggc gccgacggtt gaccccatcg ccgagggggc cgagctgtac gacgtcgtcc
    10261 ccgtcgatcc ccgggtgcct tacgacgtgc acgaggtgat cacccggatc gtcgacggcg
    10321 gcgagtttgc ggaattcaag gccgactacg gcagcacatt ggtcacggga ttcgcccgca
    10381 ttcacggcca tcccgtcggc atcgtggcca acaacggtgt gctgttcagc gaatccgcct
    10441 tgaagggcgc gcatttcatc gaactgtgtg acaagcggat gactccgctg ctgttcctgc
    10501 agaacatctc gggcttcatg gtcggccgcg attacgaagc gggtggcatc gccaagcacg
    10561 gcgccaagat ggtgaccgcc gtcgcgtgtg cgcgggtgcc gaagttgacc gtcgtcattg
    10621 gcggctccta cggcgccggc aactactcga tgtgtgggcg cgcgtattca ccacgtttcc
    10681 tgtggatgtg gcccaacgcg cgcatttcgg tgatgggtgg cgagcaggct gcctcggtgt
    10741 tggcgacggt gcgcggagaa atgaccgccg acgaggagga aaagttcaag gcgcccatcc
    10801 gtcagcagta cgaggaccag ggcaatccgt actactcgac ggccagattg tgggatgacg
    10861 gtgtgatcga cccagccgac accagaaccg tgttggggct ggcgctttcg atcgtcggcc
    10921 aggcaccgct cgaatcggtc tcttacggcg tattccggat gtgacgctga tgacttcagt
    10981 tctcgcttcg gtccttccct ccgctcctcg ctgcgttcct cgcttcgctg cggtactcgc
    11041 tgcgatgctc acgaagggcc tccgctcgtg acgaccttcg acactgttct gatcgccaac
    11101 cgcggcgaga tcgcggtgcg ggtgacccgc acgctgcggg cgatgggtat ccgctcggtc
    11161 gcagtgttca gcgacgccga tgccggcgcc cgccatgtcg cggaggcgga cgtcgcagtc
    11221 ggcatcgggc ccgcgcccgc acgacagagc tacctcaaca tcgacgccgt cgtcgcggct
    11281 gcgaagcgca ctggtgccca agcagtccac ccgggatacg gattcctttc ggagaacgcg
    11341 caattcgcgg ctgcgctaca agccgcgggc atcgtgttta tcggcccgcc tgccgcggcg
    11401 atccagacga tgggcgacaa gatcgcggcg aaggccgcgg tgtctgcgtt cggcgtgccg
    11461 gtggtgcccg gcgtgtcccg acccggactc accgatgccg atctgatcgc cggcgctgac
    11521 gaggtcggct atccggtgct gatcaagccg tcggccggcg gtggcggtaa gggcatgcgg
    11581 gtggtgcacg agccgtcgga gttggccgtg gcgctggcca gcgcacgccg cgaggcagcg
    11641 tccgcgtttg gcgatgacac gctgttcctc gagcggtttg tgttgaaccc caggcacatc
    11701 gaggtccagg tgcttgccga cggctacggc aacgttgtcc atctcggcga acgcgaatgc
    11761 agcctgcaac ggcggcacca gaaggtcatc gaggaagcgc cctcaccgct gctggacaag
    11821 gcgacccggg cgcgtatcgg cgcggccgca tgcgacaccg cccgcagtgt cgactacacc
    11881 ggcgcgggca ccgtcgagtt cattgtctcc gcggaccggc ccgacgagtt cttcttcatg
    11941 gagatgaaca cccgccttca ggtcgaacac cccgtcaccg aaatggtcac gggcatcgac
    12001 ctggtcgaac aacaagtgcg catcgcggct ggtgagaagc tgccgatcga gcaggacgac
    12061 atcaccatga ccggccacgc catcgaggct cgcgtgtatg ccgaggatcc cgcgcgcggg
    12121 ttcttaccta ccggcggcaa agtgctcgac ctcgccgagc ccgaggggcc aggcgtccgg
    12181 gtggattccg gcctcgcggc cggcatgctc atcggcagcg agtacgaccc gatgctggcc
    12241 aaagtcattg cccacgccgg cgaccggccc gccgcactgc gtgcgctcga ccgggcgttg
    12301 gcagacaccg cggtactggg cgtcacgacc aacatcgagt tcctgcgctt cctgatcgcc
    12361 gaccccgacg tcgccgccgg gcgcctcgac acggggctgt tggaccgccg aacccccgat
    12421 tacgttcctg cacagcatgg cgacgatgag ctgatcgccg cggccgccta caaatggttg
    12481 cgcggctggc cgacgccagt cggtgactta tgggaagtgc cgtcgggttg gcgaatcggg
    12541 gagcgggcgc cgacgacgtt ccggctgcat gcggccgagc gcacagacca cgtgtatctc
    12601 accggcacac cgcaggccgc gaccgctcgc gtcgagcacg gcggaagcca ttcgctgaca
    12661 gcgtctctcg acgatgaccg gctggcggtc acgctggacg ggttgcgcac cgactacctc
    12721 gtcgccgcca ccgaccatca gatctggctg tccggcggcg gtcgcaccgc gatggtcgag
    12781 gagatgcggg aagcacccgt gcgccccgac gatgaacaca gcggcgatgc cgagctgacc
    12841 agcccgatgc ccggttcggt tgtggccgtt ggcgttgagg acggccaaag cgtggacgcc
    12901 ggcacggtcg tcgtcaccgt cgaagccatg aagatggagc atgcgctgtc cgcgcccgtc
    12961 gacggcgcag ttgagcttct cgtcgccgtc ggcgaccagg tcaaggtggg ccagccgctg
    13021 gccaggatca ccgcaagtac gaaagaagac gaatcatgag cgattttctg gccaccggaa
    13081 cactgccgga tcactacgaa caactcgcca agaccgtccg tgacttcgcc caaagcgtgg
    13141 tcgcgcccgt ctcggccaag cacgacgagg agcattcgtt cccgtacgaa gtcgtgtcgg
    13201 ggatggcgga catggggttg ttcggcctgc cgttccccga ggagtacggc ggcatgggcg
    13261 gcgtctactt cgcgctgtgc ctggcgctcg aggaactcgg caaggtcgac cagagcgtgg
    13321 cgatcacact ggaggcgggt gtgtcgctgg gcgccatgcc ggtgtaccgg ttcggcaacg
    13381 aagagcagaa gcaggaatgg ttgccgctct tggcaagtgg caaggcgctc ggtgcgttcg
    13441 ggctgaccga ggcgggcggc ggaaccgatg ccggtgccac caggaccacc gccaagcttg
    13501 acgacggaca ctgggtcatc aacggctcca agcagttcat caccaactcg ggcaccgaca
    13561 tcaccaaact ggtgaccgtc accgccgtta ccggagacac cggtggcagg aaagagatct
    13621 cgtcgatcct ggtgccggtg cccaccgagg gcttcaccgc ggagcccgcg tacaacaaag
    13681 tcggctggaa tgcctcggac acccacccgt tgtcgttcga cgacgtccgg gtgcccgccg
    13741 agaatctgct cggagagcgc ggccgcgggt acgccaactt cctgcgcatc ctcgacgagg
    13801 gccggatcgc catcgctgcg ttgtcggtcg gtgccgccca gggctgtgtc gacgagtgcg
    13861 tgaagtacgc caaggagcgc caggcgttcg gtgccgcgat cggcacctat caggccgtcg
    13921 cgttcaagat cgcacgcatg gaggcccgcg cacacgccgc ccgcactgcg tattatgacg
    13981 ccgccgcact catgttggct ggcaagccgt tcaagaaggc cgcggcgatc gccaagctcg
    14041 tcgccagcga ggccgcgatg gacaactctc gggatgccac ccaggtgttc ggcggctacg
    14101 gcttcatgaa cgaatacgcc gttgcgcgcc attaccgtga cagcaagatc ctcgagatcg
    14161 gcgaagggac aacggaagtg cagctgatgc tcattgcccg tgaggcaggc ctgtgaccga
    14221 taagaaggtc gttgttcagc gcgggctgtg gttcgaggag ttcgagaccg gcgtgctgta
    14281 tcagcaccgc ccgggccgca ccatcaccga agccgacaac gtgctgttca ccacgctgac
    14341 gatgaacacc caggcgctgc acctcgacgc cgcgttctcc gacgcgttgc cgccgttcaa
    14401 ccagcggctg gtgaattcga tgttcacgct ctcgacgctg gtcggtctgt cggtggcgca
    14461 gctgacgcag ggcacgatcg tgggcaacct gggcttcggc gaggtcgcgt tccccaagcc
    14521 gctcttccac ggtgacacgc tgtacgccga gtcggaggtg acggacaagc gggagtccaa
    14581 gagccggccc ggcgaaggta tcgtgacgtt cgcacacacc ggccgcaatc agcacggcga
    14641 catcgtggcg accgcatcgc gaaagaccat ggttcgtaag aagccggagg agtgatggcg
    14701 ttgacgcaga ccggtccggc gtggttgttc tgcccggccg atcgccccga gcgattcgaa
    14761 aaggccgccg cggcagccga tgtggtgatc ctcgacctcg aagatggcgt tgcggccaag
    14821 gatcgggaag cggcccgcag ggcgttgatc gagacgccgc tggacccgga ccgcaccgtc
    14881 gtgcgcatca atccgagcgc tactgccgac catcagctcg acctcgaagc gttgaagcgc
    14941 acttcgtaca cgaccgtgat gctggcgaaa accgaatccg tgcaacaggt cagcgcgctg
    15001 ctgccgctgg atgtggtggt gctgatcgaa acgccgctgg gagcgctgca cgtcgtcgaa
    15061 ttgtcgcgcc ttggcaacgc tatcgccctg atgtggggtg ccgaggatct gttcgcggtg
    15121 ctgggtggga ccgccaaccg tcacgttgat ggtacctatc gcgacgtcgc tcggcacgtg
    15181 cggtcgcaga cgctcctggc ggccaaggcg tacgggtgca tggcgctgga ttcggtgttc
    15241 atcgacatca aggacctcga cggtctgcgt gacgaggtcg acgacgcggt ggcggtcggc
    15301 ttcgatgcga aggtcgcgat ccacccgtcg caggtggcgg tcattcgtgc cggctatgcg
    15361 cccaccgcgg agcaggtgga atgggcgcga cacgtgctgg cggcagcccg cgatgagcgg
    15421 ggcgtgttcc aatacgaggg cttgatggtc gatgccccag tacttcggcg cgccgaacgg
    15481 atcgtcgcgc tcgcgccgaa cgatcgtggc tggtagcgat cctggagatg cgggtcctcc
    15541 cgcggagttt ggcggttcgg gcgacctcgg tcggtgtgaa tcggcacacc gcatagtgct
    15601 gctttcggag ggaacgctgc cggtgcccgc ggagcgggtg ggccaaatga ttctggtcaa
    15661 gaccagggaa gcatcggcct cacggcgggc ctggtggggt atccgcgggt gtcaacgcat
    15721 gatcagcgct gggatctgtg ccggaaggtg gcccggttga gcggttgggc gagcggtgga
    15781 ggtggccgct gtagacatta gtcaacgtcg gcgcgtttac cagatcgtcg atcaggtcgt
    15841 cctcggcggt gatggcgttg agcaccaacg aaactggatc attgacagcc tctcggcgat
    15901 cgcgtgcgcc gacgggctca cttggtcacc tcctggctct tgccgacgac ggtcacgccg
    15961 ccgtcggaga cgacgaatcc gcgcgcccgg tcgtgatcct tgtccacccc cacctgcgcc
    16021 ccctcgggta ccactacgtc cttgtcgatg atcgcgttct gtaccacggc gttggcggcg
    16081 acaacggtgt tgtccatgat cactgagcgt tccactatgg cgtagtcctc tatccgcgcg
    16141 ccgggagaca gtactgactc gcgcaccagc gccccagaga cgatgacgcc gttggagacc
    16201 acgctgttga tcgcatggcc gacccgctcg ccgtcctcgt gcacgaactt cgccggtggg
    16261 tgcggcggga tgtgggtgag aatcggccac tcactgttgt acaggttgaa taccggcacg
    16321 acagcgcaca ggtccatatg cgcgtcgtag taggagtcca acgttccaac gtcccgccag
    16381 tagccggcgt cggcagggtc ggcgccgggc acctcgttgc gttgaaagtc gtacacctgt
    16441 gccatgcccc ggtccaccat catcggcatg atgttgccgc ccatgtcgtg cttgctggac
    16501 tcgtcgtcgg cgtccttgcg cagcacctcg accagcgcgt cggcggagaa caggtagttc
    16561 cccatcgacg cgaacgtctc gtcgggccga cctggcagcc ccggcgggtc gggcggcttc
    16621 tccaggaacg cctcgacccg gagcccgtcg gagccggtct tgatcacgcc gaaggcgctg
    16681 gcctcctcgc gtggcaccgg aatgccggcc accgtcgcgc ccgcgccact gtcgaggtgc
    16741 tgcgcgatca tctgcctcgg gtccatgcgg tagacgtggt cggcgccgaa cacgatgacc
    16801 agatccggct tctcgtcgtg gatcaggttc agtgactggt agatcgcatc ggcggagccg
    16861 gtgtaccact gcgggccaag gcgctgctgg gcgggcaccg gggtcacgta gttggcgagc
    16921 aagctgctca tccgccaggt cgtggtgatg tggcggtcca ggctgtggct cttgtactgg
    16981 gtgagcaccg cgatccgcag caccccggcg ttgaccaggt tcgacaacgc gaaatcgatc
    17041 aatcgataca gcccaccgaa cggaacggct ggcttagccc ggtcgagggt cagtggtgag
    17101 aggcgcttgc cctcaccacc ggcgagcacg atgccgagca cctttggatc ggccatctga
    17161 cgtctccttc tgttggacgc ttcagttgga cgtgttgagc gcgcggccga cgatcttgcg
    17221 ggcctccagc tggcccgggc caggcgaacg actcggcgct ccccgcctaa cgccgccgag
    17281 ccgtccaccg gccccggcgc gaaccacttg aacctggtct cgtcggaggc ctcgggctac
    17341 gactcttggc gatgtctgtt tgctgggcca cgtcctcgag agaatcatcc cgtggtagcc
    17401 acgcgaggaa agcacccggg ccgtcgttgt cgcgggcaaa atgtcggaag ctgcggtcgt
    17461 cagaggttca gccaccgaaa gtcgctttcg tcgaggactg gaaccggcga tgccagcctc
    17521 gtgaacagac ccagtctaga cgcaccgacc gaccgggcag ctctttcttc gatccactca
    17581 cccttgtccg attcgttagt tggcctccag agcacattag gtgcaatagt cgaagttgcg
    17641 cgtagggagt aaagggaatg acgcggacca accaagtcac cagtccgcat ctgcggccgc
    17701 atacggcgga tctgaaccgc ttgctggccg gcgagcacca tgatccgcat tcggtgctgg
    17761 gcgctcacga gtacggcgat cacaccgtga ttcgggccta tcggccgcat gcggttgagg
    17821 tggtc
//
LOCUS       DASRJA010000290         4889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3991129,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000290 DASRJA010000000
VERSION     DASRJA010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3991129"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFC01_12710"
     CDS             complement(<1..647)
                     /locus_tag="VFC01_12710"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217584.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HZN80531.1"
                     /translation="MAANPRAGQPAQPEDLIDVAQVVTAYYTVEPDPDNVDQQVVFGT
                     SGHRGSSLDAAFNEAHIIATTQAIVEYRAAQGTTGPLFIGRDTHALSEPAWASALEVL
                     AANDVVAMIDSADRYTPTPAVSHAILTFNRDRDGDLADGIVVTPSHNPPRDGGFKYNP
                     PNGGPADTDATGAIAKRANEILRDGLKGVKRLPLARALQSAQRHDYMDAYVEDLP"
     gene            719..1117
                     /gene="crcB"
                     /locus_tag="VFC01_12715"
     CDS             719..1117
                     /gene="crcB"
                     /locus_tag="VFC01_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HZN80532.1"
                     /translation="MFSFDGRELAAVFVGGALGTLARAAFETLAAPDPGRWPWPTFTV
                     NIVGAFLLGYFTTRLLERLPVSSYRRPLLGTGVCGGLTTFSTMQVETIKMLEHHHYAL
                     AATYTAVSIVVGLLALYLATALVRRARIRA"
     gene            1114..1479
                     /gene="crcB"
                     /locus_tag="VFC01_12720"
     CDS             1114..1479
                     /gene="crcB"
                     /locus_tag="VFC01_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HZN80533.1"
                     /translation="MTVAVWAGVVVVGGVGAVLRFVVDRTVSSRTAGSFPYGTLAVNL
                     SGALLLGFLSGLALSPHLALLAGTAFVGSYTTFSTWMLETQRLAEERQLRPAVANIVV
                     SVVLGLAAAWVGLWIGGRF"
     gene            1476..2540
                     /locus_tag="VFC01_12725"
     CDS             1476..2540
                     /locus_tag="VFC01_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HZN80534.1"
                     /translation="MRDEYLKLTAYFAERLRCDDRFVADALLDLFGESAVATSVVLRG
                     IASFGPHHVLRSDQSLSASEDSPIAVAAVDRSDKIAPLADRAVAMTTRGLLTLERARL
                     LGGPDLSTPPDTVKLTVYIGRQDRVADRPAHQTVCDVLYRHGFAGASVFLGVDGTRSG
                     TRRRARFFSRNVDVPVMIIAIGTGIQATAAIPELEAMLRQPLLTTERVQLCKRDGELV
                     TRPAAMPMADDHGRALWQKLMVYTSEATLHEGVPIHRAIVQRLFDANTASGATVLRGI
                     WGFHGDHKPHGDKMIQLTRQVPVTTIVVDSPDRIAASFDIIDELTDRHGLVTSELVPA
                     LVSIDDGERRGGTELARYHY"
     gene            2543..2740
                     /locus_tag="VFC01_12730"
                     /pseudo
     CDS             2543..2740
                     /locus_tag="VFC01_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514496.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            complement(2704..2960)
                     /locus_tag="VFC01_12735"
                     /pseudo
     CDS             complement(2704..2960)
                     /locus_tag="VFC01_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            3080..4495
                     /locus_tag="VFC01_12740"
     CDS             3080..4495
                     /locus_tag="VFC01_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HZN80535.1"
                     /translation="MNEVLERELKFDVDEQFALPRLDDIAPGAGVEHSTVTLNSAYYD
                     TADGDLQSHGVLLRRRDGDDDTGWQLKVPHTEGRVEIRTGLSDTPPSELTDALTGLRL
                     GKPLLNVATVRTTRDRYRITDPRRDRLCAEVADDHVRASIDHRLLAWREIEVELGPGR
                     RSVPRRLTERLVEAGATPTRFPSKLARVSGVTRPEPAGETAADRAVATYMTEQIDNIF
                     DGDLGLRRGSDPIHDTRVAIRRLRSTVRVFGKLMDSSVGHIDDELKWFAGLLGEVRDC
                     QVQRRRFREALAQLPPELVLGPVANRINTDLQSDQLQARTVAAEAMNSQRYLDLLATL
                     QHWRTGPPLIEPPSVKAVTKRAHRAERKADRRLAEAIEAGDDALLHRARKAAKRARYA
                     AELRKPLDKRAKETAKHYKQFQRVLGDHQDGVVASATLIRLARAAGTTPGENGFAFGL
                     LYAREQQAAEEARNRVRELIG"
     gene            complement(4492..>4889)
                     /locus_tag="VFC01_12745"
     CDS             complement(4492..>4889)
                     /locus_tag="VFC01_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN80536.1"
                     /translation="FDGEFVHFDAVRVNPRPVANRRIPIVLGGNSDAALRRVVAWGDG
                     WYGFNLDGVREVAERMDFLRRLCDDTGRDRAELQLAVALRDLSVKDVRPLAELGVDEL
                     VIVEGPPDDAADAGTWVSALADEWIPAAR"
BASE COUNT          805 a         1660 c         1598 g          826 t
ORIGIN      
        1 ctgggcaggt cctcgacgta agcgtccatg tagtcgtggc gttgcgcact ctgcaacgcc
       61 cgggccagcg gcagccgctt cacacccttg agcccgtcgc ggaggatctc gttggcccgc
      121 ttggcgatgg ccccggtcgc gtcggtgtcg gcgggtccac cgttgggcgg gttgtacttg
      181 aagccgccgt cgcgcggcgg gttatgcgac ggagtcacca cgatgccgtc ggccagatcg
      241 ccgtcgcggt cacggttgaa cgtcaggatc gcgtggctga ccgcaggcgt cggggtgtag
      301 cgatcggccg aatcgatcat cgcaacaacg tcattggcgg ccagcacctc cagcgcggag
      361 gcccacgccg gctccgacag ggcgtgtgtg tcacggccga tgaacagcgg tccggtggtg
      421 ccttgcgccg ccctgtactc gacgatcgcc tgggtcgtcg cgatgatgtg cgcctcgttg
      481 aaggccgcgt ccaggctcga gccgcggtgc ccggacgtgc cgaacaccac ctgttgatcc
      541 acgttgtccg ggtcgggttc gacggtgtaa tacgccgtca ccacctgggc gacatcgatg
      601 agatcttcgg gttgtgccgg ctgaccggca cgcgggttcg cagccatgtc cccgattctg
      661 ctcctctgag aatcgcgtcg cgcgcgtgtc cggaagctcg acggcgatac tttggctcat
      721 gttcagtttc gacggccggg agctggccgc cgtcttcgtc ggcggcgccc tcggcaccct
      781 cgcgcgcgcg gcatttgaaa cgcttgcggc gcccgacccg gggcgctggc cctggccgac
      841 gttcaccgtc aacatcgtcg gcgcatttct gttgggctac ttcaccaccc ggttgctcga
      901 gcggttgccg gtgtcgagct atcggcgccc gctgctcggc acgggcgtct gcggcggcct
      961 gaccacgttc tcgacgatgc aggtagagac catcaagatg cttgagcacc accattacgc
     1021 ccttgccgcc acgtacaccg cggtgagcat cgtggtcggg ttgctcgccc tctatctcgc
     1081 aaccgcgctg gtgcgacgcg cccggatccg cgcgtgacgg tcgcagtatg ggctggcgtc
     1141 gtcgtggtcg gcggcgtcgg cgcggtgttg cgcttcgtcg tcgaccgcac ggtgtcgagc
     1201 cgaactgccg gatcgtttcc gtacggcacc ctggcggtca atctcagcgg cgccctgctg
     1261 ctcggcttcc tgtccggtct cgcgctcagc ccacacctcg cgctgctggc cggcaccgca
     1321 ttcgtcggct cctacacgac cttctcgacc tggatgctgg agactcagcg cctcgccgaa
     1381 gagcgccagc tgcggcccgc cgtcgccaac atcgtcgtca gcgtggtgct tggcctggcc
     1441 gcggcctggg ttggcctgtg gattggaggc cggttttgag agacgagtac ctcaagctca
     1501 ccgcctactt cgccgaacgt ctgcgctgcg atgaccgctt cgtcgccgac gcgctgctcg
     1561 atctcttcgg tgagagcgcc gtggccacca gcgtggtgct gcgtggcatc gccagcttcg
     1621 gcccgcacca tgtgctgcgc agcgaccagt cactgagcgc atcggaggat tcgccgatcg
     1681 cggtcgcggc ggtcgaccgt agtgacaaga tcgctccgct cgccgaccgg gctgtcgcga
     1741 tgacgacacg cggtctattg acgctcgagc gcgcacgtct gctcggtggg ccggatttgt
     1801 cgacaccgcc tgacaccgtc aaactcacgg tctacatcgg gcgtcaagac cgggtcgcgg
     1861 accgacccgc gcaccaaacc gtttgcgatg tgctgtaccg gcacggattc gctggagcct
     1921 cagtatttct cggcgtcgac ggcaccagaa gcggaacacg gcggcgggcc cgcttcttca
     1981 gccgcaacgt cgacgtgccc gtgatgatca ttgcgatcgg aactggcatc caggccaccg
     2041 cggcgatccc ggagcttgag gccatgctgc gccagcctct attgacgacg gaacgggtgc
     2101 agctctgcaa gcgcgacggt gaactcgtga ctcggcccgc cgcaatgcca atggccgacg
     2161 accacgggcg cgcgctgtgg cagaagctga tggtctacac gtcggaggcc acgctgcacg
     2221 agggcgtccc gatccaccgc gccatcgtcc agcggctgtt cgatgccaac accgccagcg
     2281 gcgcgaccgt gctgcgcggc atctggggat ttcacgggga ccataaaccg cacggcgaca
     2341 agatgattca gctgacccgc caggttccgg tgacgacgat cgtcgtcgac agccctgacc
     2401 gcatcgccgc gagcttcgac atcatcgacg aactcaccga ccggcacgga ttggtcacga
     2461 gcgagttggt tcccgccttg gtgtcgatcg acgacggcga gcggcgtggc ggaacagagt
     2521 tggcccgata ccactactga ccgtgaacga gtgcatgcgc atcttcggcg ggtcgggcta
     2581 cctggtcgat gaaacgccgc tgggccagtg gtggcgcgac atgaagctcg cgcgcgtcgg
     2641 cggcggcacc gacgaggtgc tgtgggaact ggtcgccgcg gcgatgaaac cggactacga
     2701 cggctacgcc gagattatcg actcgcgtcg caatgcgtag agcgccatgc gccggttgga
     2761 catgtcgtgc tcgcccagga agtcacagcc cgcgtattcg cacagccggc gggcaccgct
     2821 gttgcggtga tccggatcga acatgattcg gcggcaacgt ggttcgagct cgaagtgctc
     2881 gcgacgacgc gtggtaacag gatcggcgcg atgccgcggt tgacgaactt cagattcgcg
     2941 atcgcggcgt gcagtccgat cctcatcaat ctcggtcatt tgagcgccga gcctacctca
     3001 gtaagttagg taacacttac ctcccttctg accgccgttc tgatgttttg ccgacgctgg
     3061 atgcggggag cctttcgcca tgaatgaagt gctggaacgc gaactcaagt ttgatgtcga
     3121 cgagcaattc gcactccccc gtctcgacga catcgccccc ggagccggcg tggaacacag
     3181 cacggtcaca ctcaacagcg cctactacga caccgccgac ggggacctgc agtcacacgg
     3241 agtgctgctg cggcggcgcg acggcgacga cgacaccggc tggcagctca aggtgcccca
     3301 caccgagggt cgcgtcgaga tccgcaccgg gctctccgac acaccgccgt ccgagctgac
     3361 cgacgccctc accggcttgc ggctgggcaa gccgttactg aacgtggcga cggtgcgcac
     3421 gacccgcgac cggtatcgga tcaccgaccc ccggcgtgat cggctctgcg ccgaagtggc
     3481 cgacgatcat gtccgcgcct cgatcgacca tcgcctgctg gcgtggcggg agatcgaggt
     3541 cgagttgggc cccggcaggc gctcagttcc ccgccgcctt accgaacggc tggtcgaggc
     3601 cggcgctacc ccaacgcgat tcccgtcgaa acttgcccgc gtctcgggcg tgacgcgacc
     3661 cgaacctgcg ggtgagacag cggccgaccg agcggtggcg acgtacatga cggagcagat
     3721 cgacaacatc ttcgacggcg atctcggcct gcgccgcggc agcgatccga ttcatgacac
     3781 gcgagtggcg atccgccggc tgcgcagcac tgtgcgggtg ttcggcaagc tgatggattc
     3841 gtcggtcggg cacatcgacg acgaactcaa gtggttcgcc ggcctgctcg gggaggtccg
     3901 cgattgccag gtgcaacgcc gccgcttccg agaagcgttg gcacagttgc cgcccgagct
     3961 cgtcctcggg ccggtcgcca accggatcaa caccgatctg cagtcggacc aactgcaggc
     4021 ccgcacggtc gctgcggagg cgatgaactc gcagcgatac ctcgacctgc tcgcgacgct
     4081 gcagcattgg cgcaccggtc cgccgctgat cgaaccaccg agtgtaaaag cggtgaccaa
     4141 gcgcgcccac cgcgcggagc gcaaggccga tcggcgactg gccgaggcga tcgaggcggg
     4201 cgacgacgcg ctgctgcacc gcgcccgcaa ggccgccaaa cgcgcgcgat atgccgccga
     4261 gctccgcaag cccctcgaca aacgcgcaaa ggaaacggcc aagcattaca agcagtttca
     4321 gcgcgtgctc ggtgaccacc aggatggcgt tgtcgcatcg gccacgctca tccggcttgc
     4381 ccgcgctgcc ggcacgacgc caggcgagaa cggattcgcc ttcgggcttc tctatgcgcg
     4441 cgaacaacag gccgccgaag aagcgcgtaa ccgggtgcgg gaactgattg gctagcgcgc
     4501 tgccggaatc cactcgtcgg ccagcgccga tacccaggtc cctgcgtcgg ccgcatcgtc
     4561 gggcggaccc tcgacgatca ccagttcgtc gacaccgagc tcagcgagcg gccgaacgtc
     4621 tttcacgctc aggtcacgaa gcgccacggc cagctgcagt tcagcgcggt cgcgtccggt
     4681 gtcgtcacac agccgacgga ggaaatccat ccgttcggcg acttcgcgca caccgtcgag
     4741 gttgaaccca taccagccgt cgccccaggc gacaacccgg cgcagcgctg catcactgtt
     4801 gccgcccagc acgatcggaa tgcgcctgtt ggcaacgggt ctggggttga cccgtaccgc
     4861 atcgaaatgc acgaactcgc cgtcgaacg
//
LOCUS       DASRJA010000291         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_818559,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000291 DASRJA010000000
VERSION     DASRJA010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818559"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="VFC01_12750"
     CDS             complement(<1..1435)
                     /locus_tag="VFC01_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HZN80537.1"
                     /translation="MTATVSTSVASSWIDGAPVITGGGLHQVINPATGEAVADYALAT
                     PADVDRAVASARAALPAWATATPADRSAVLARLAKLADEHADELVAEEVSQTGKPVRL
                     ATEFDVPGSVDNIDFFAGAARHLEGKATAEYSGDHTSSIRREAIGVVGTITPWNYPLQ
                     MAVWKVLPALAAGCAVVIKPCELTPLTTLTLARLASEAGLPDGVFNVVTGLGADTGTA
                     LAGHRDVDVVTFTGSTAVGRKVMAAAAVYGHRTQLELGGKAPFVVFDDADLDAAIQGA
                     TAGSLINTGQDCTAATRAIVARDLYDDFVAGVGELFGKVVVGDPQDPDTDLGPLISMA
                     HRAKVAGMVERAPGEGGRIVAGGSAPDLPGSFYLPTVIADVSENSEVYRDEIFGPVLT
                     VRSFTDDDDALRQANDTEYGLAASAWTRDVYRAQRASREIQSGCVWINDHIPIISEMP
                     HGGVGASGFGKDMSDYSFEEYLTIKHVM"
     gene            1558..2079
                     /locus_tag="VFC01_12755"
     CDS             1558..2079
                     /locus_tag="VFC01_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZN80538.1"
                     /translation="MANPGAPHSMAPVSLRVNTSRPGAAFQLDELSKQIIEKLQQDGR
                     RSYAGIGKAVGLSEAAVRQRVQRMVDAGVMQIVAVTDPMQLGFARQAMIGIRCTGDTT
                     RVAEKLAAIESVDYVVLTAGSFDAIVEVVCEDDDDLLDLLNTQIRALPGVISTETLVY
                     LKLVKQQYNWGTR"
     gene            2076..>3269
                     /locus_tag="VFC01_12760"
     CDS             2076..>3269
                     /locus_tag="VFC01_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZN80539.1"
                     /translation="MTTTDITQEISSELGAKANRHLWGHFARHGEGITPPIITRGEGV
                     TIWDDNGKSYIDGLSGLFVVQVGHGRKELAEAAAKQAETLAFFPLWSYGTPPAIELSE
                     RIANYAPGDLNRVFFTTGGGEAVESAWKLAKNYFKLTGKPGKHKVVSRSIAYHGTPQG
                     ALAITGLPVFKEPFEPLTPGGFRVPNTNFYRAPAPYEHDIKAFGQYCADRIAEAIEFE
                     GPDTVAAVFLEPVQNAGGCFPPPPGYFERVREICDEYNVLLVSDEVICAYGRIGSMFA
                     CDDFGYVPDIITCAKGLTSGYSPIGAMIASDRLFEPFNDGKTTFAHGYTFGGHPVSSA
                     VALANLDIFEREGINDHVKETAPAFRATLEKLYDLPIVGDVRGEGFFYGIELVKDKAT
                     KETFND"
BASE COUNT          581 a         1100 c         1048 g          540 t
ORIGIN      
        1 tcatcacgtg cttgatcgtg aggtactcct cgaaggagta gtcggacatg tccttgccga
       61 accccgatgc accgacgccg ccgtgcggca tctcgctgat gatcgggatg tggtcgttga
      121 tccacacaca gccggactgg atttcgcgcg acgcgcgctg cgcccggtac acgtcgcgcg
      181 tccacgctga ggcggccaaa ccgtactcgg tgtcgttggc ctgacgcagc gcatcgtcgt
      241 cgtcggtgaa cgagcggacc gtcagcaccg gcccgaagat ctcgtcgcga tagacctccg
      301 agttctcgga gacatcagcg atcaccgtcg gcaggtagaa cgaacccggc agatccggtg
      361 cgctgccgcc cgcgacgatg cgcccgccct cgccgggcgc ccgctccacc atgccggcca
      421 ccttcgcgcg gtgcgccatc gagatcagtg ggccgaggtc ggtgtcgggg tcttgcggat
      481 cgccgacgac gaccttgccg aacagctcgc cgacgccagc gacgaagtcg tcgtagaggt
      541 cacgcgccac gatcgcgcgc gtcgcggctg tgcagtcctg cccggtgttg atcaatgagc
      601 cggcggtcgc gccctggatc gcggcgtcca gatcggcgtc atcgaacacg acgaacggcg
      661 ccttcccgcc gagctccagc tgagtgcggt gaccgtacac cgccgcggcg gccatcacct
      721 tgcggccgac agccgtcgac ccggtgaacg tcaccacgtc cacgtcgcga tgcccggcca
      781 gcgcggtgcc ggtgtcagca ccaagccctg tcacgacatt gaacacgccg tcgggcagcc
      841 ccgcctcgct ggccaaccgg gccagcgtca gcgtcgtcag cggggtcagt tcgcaaggct
      901 tgatgacgac ggcgcagcca gcggctaagg cgggcaacac cttccacacc gccatctgca
      961 gcgggtagtt ccacggcgta atggtgccga ccacgccgat cgcttcgcgg cggatgctcg
     1021 acgtgtggtc gccggagtac tccgcggtgg ccttgccctc gaggtggcgg gccgcgccgg
     1081 cgaagaagtc gatgttgtcg acgctgcccg gcacgtcgaa ctcggtggcc agccgcaccg
     1141 gtttaccggt ctgactgacc tcctccgcga cgagctcatc ggcgtgctcg tcggccagct
     1201 tggccagcct ggccaaaacg gcggagcggt cggcaggcgt cgccgtcgcc caggcaggca
     1261 atgccgcgcg cgccgacgcc acggcccggt ccacatcggc gggcgtcgcc agcgcgtaat
     1321 cggcgacggc ctcaccggtc gcggggttga tcacctgatg caggccgccc ccggtgatca
     1381 ccggggcgcc gtcgatccag ctgcttgcca cgctggtgga tacggtcgca gtcatgggcc
     1441 aacgctaacc gacgagggac acgaaggcta cgcattcccg ctgcgcgagg ccctccaaac
     1501 gagggatttc atagctacgg ttgattgtga cgacggattc cgtgcacaat cacaggcatg
     1561 gctaacccgg gtgcaccgca ttcaatggcg cccgtctcgc tccgcgtcaa cacgtcccga
     1621 cctggcgcag cgttccaact tgacgagctg tcgaagcaga tcatcgagaa gctgcagcag
     1681 gacggccgcc ggtcgtacgc gggtatcggc aaggcggtcg ggctgtccga ggccgcggta
     1741 cgccaacgcg tccagcgtat ggtcgacgcc ggcgtcatgc agatcgtggc ggtcaccgat
     1801 ccgatgcagc tgggcttcgc ccgccaggcg atgatcggca tccgctgtac cggggacacc
     1861 acccgggtgg ccgagaagtt ggccgccatc gaatccgtcg actacgtggt gctcaccgcg
     1921 ggctcgttcg acgccatcgt cgaagtcgtg tgcgaggacg acgacgacct tctggaccta
     1981 ctcaataccc aaattcgcgc attgccggga gtgatatcca ccgagacact tgtttacttg
     2041 aaactcgtta agcagcaata caattggggt acaagatgac aactaccgat atcacacaag
     2101 agatttcatc cgagctgggc gcaaaggcca accgtcacct gtggggccac ttcgcccgcc
     2161 acggcgaggg catcacgccg ccgattatca cccgcggcga gggcgtgacc atctgggacg
     2221 acaacggaaa gagctacatc gacggcctct caggcctttt cgtcgttcag gtcggccacg
     2281 gccgcaagga gctcgcggag gcggcggcca agcaggccga gacactggcc ttcttcccgc
     2341 tgtggtcgta cggcacaccc ccggcgatcg agttgtcgga gcgcatcgcc aactatgcgc
     2401 ccggcgatct caaccgggtg ttcttcacca ccggtggcgg cgaggcggtc gagtcagcgt
     2461 ggaagctggc caagaactac ttcaagttga ccggcaaacc cggtaagcac aaggtggttt
     2521 cgcgttcgat cgcctatcac ggcacgccgc agggcgcgct ggcgatcacc ggcctgccgg
     2581 tgttcaagga gccgttcgaa ccgctgaccc ctggtggctt ccgcgtgccg aacacgaact
     2641 tctaccgtgc gcccgcgccc tacgagcacg acatcaaggc gtttggtcag tactgcgcgg
     2701 accgcatcgc cgaggccatc gagttcgagg gacccgacac cgtcgcggcc gtcttcctcg
     2761 aacctgtgca gaacgcgggc ggctgcttcc cgccgccccc agggtatttc gaacgggtcc
     2821 gggagatctg cgacgagtac aacgtgctgc tggtgtcgga cgaggtgatc tgcgcgtacg
     2881 ggcggatcgg atcgatgttc gcctgtgatg acttcggcta tgtgcccgac atcatcacgt
     2941 gcgcaaaggg tctgacgtcc ggctactcgc cgatcggtgc gatgatcgcc agcgataggt
     3001 tgttcgagcc gttcaacgac ggcaagacca cattcgccca cggctacacg ttcggtggac
     3061 accccgtgtc gtcagcggtg gcgctggcca acctcgacat cttcgagcgc gagggcatca
     3121 acgaccatgt caaggagact gcgcccgcat tccgcgccac gctggagaag ctgtacgacc
     3181 tgccgatcgt cggcgacgtc cgcggcgaag gcttcttcta cggcatcgag ctcgtcaagg
     3241 acaaggccac caaggagacg ttcaacgac
//
LOCUS       DASRJA010000292         8149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1463825,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000292 DASRJA010000000
VERSION     DASRJA010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463825"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..82
                     /locus_tag="VFC01_12765"
     CDS             <1..82
                     /locus_tag="VFC01_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HZN80540.1"
                     /translation="AVSHRGRALAALLPVLRELATGRTSA"
     gene            complement(60..395)
                     /locus_tag="VFC01_12770"
     CDS             complement(60..395)
                     /locus_tag="VFC01_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HZN80541.1"
                     /translation="MTEAPTTPVETIRKALLGYRVMAWTTGLWLIALCYEMVLKYIVQ
                     VDNPPNWIAIVHGWVYFVYLIFTANLAVKVRWPIGKTIGVLLAGTIPLVGIIVEHFQT
                     ADIKQKFGL"
     gene            complement(392..763)
                     /locus_tag="VFC01_12775"
     CDS             complement(392..763)
                     /locus_tag="VFC01_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80542.1"
                     /translation="MSTTRRRRPALIALVIVAAAGCLALAWWQWTRFESASGTFQNLG
                     YALQWPLFAGFCFYAYYKFVRYEDAPPEPRNHDAVTEIPSGILPERPKPATPSDDDTT
                     LREYNAYLADLAKQDKEHPTP"
     gene            complement(744..1244)
                     /locus_tag="VFC01_12780"
     CDS             complement(744..1244)
                     /locus_tag="VFC01_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80543.1"
                     /translation="MLDKKYPAIIDRCREAIDMLMPRQRAAVQSLPKGCVEVSLYSKH
                     WPCLLPQHGPGKKHMRRIALEPWQQVLVDQATEEFILGLIHSDGCRVVANDRGVMSIR
                     YHFSNRSEDIIGLFTAALDTLDIPWTRSTKYIVSIYRKAATARLDSFIGPKGRAVPLN
                     GVHYAA"
     gene            1583..1666
                     /locus_tag="VFC01_12785"
     tRNA            1583..1666
                     /locus_tag="VFC01_12785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1617..1619,aa:Leu,seq:tag)
     gene            complement(1680..2315)
                     /locus_tag="VFC01_12790"
     CDS             complement(1680..2315)
                     /locus_tag="VFC01_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80544.1"
                     /translation="MPAELVIACSDSTRTNREDEGASPELIAYDTKIRELEQLFRTAV
                     ATSLASIPIADRTSRLNGITGHIAESVVASLLEDLGYTPLQQMIGPESGGHGVDLLMA
                     TPGFEAVFSIEVKGTLQRGRWPRLTRGEIAQMTEEWLDKSDNPGMADHDLAGSDVYGA
                     VVLVNFARRQWKTGVTADFRDAYAITNRAQLEDVTWLIDTNLGTPPLAELP"
     gene            complement(2525..3964)
                     /locus_tag="VFC01_12795"
     CDS             complement(2525..3964)
                     /locus_tag="VFC01_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN80545.1"
                     /translation="MSTLADQTRWMDARDQAALVASGELSPGELLEAAIERIERIDPA
                     LNAVVIRWFDHARETAASADLAYGPFRGVPFLIKDLFATYAGQRISNGNVAFKEADVI
                     AGADTTLVSRYRAAGLVIAGRTNSPELGSVPTTEPVAWGATHNPWDTTRTPGGSSGGA
                     AAAVASGMVPFAHASDGGGSIRIPASCCGLVGLKPSQGRITLGPLRDESGLAVEHCVS
                     RTVRDTAALLDATHGPGIGDTVIAPPPSRPYVEELGADPGRLRVGILDDHPQGGRVDA
                     EVTHAAQAVGKLLESLGHHVEPAWPKALGDKTFGSMFGALWSANMGMSLRRFEEQLGR
                     PLEDRELEPMNRVQADFAKRITAVDYGLALSAVAQYRRAVQSWWHDGWDLLLTPTVAE
                     VPIKLGTIVNDPDNPMSPMRRAGDFVPFTPPFNTSGQPAISLPLEWTAEGLPVGVQLV
                     AAYGREDVLIRVASQLEQAKPWAHRTPEI"
     gene            complement(4114..4863)
                     /locus_tag="VFC01_12800"
     CDS             complement(4114..4863)
                     /locus_tag="VFC01_12800"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZN80546.1"
                     /translation="MSRLNGKVAVITGGSSGIGLATAQRFVDEGAYVFITGRRQRELD
                     KAKALIGDGVTTVAIDSTSGADLDKLFATVLEEEGGLDILVVNSGRVEPEELGKITEE
                     NFDRTFDLNARGSLFTVQKALPLMRTAGSVILVGSIAGYMGIPGYSTYSATKAALRSY
                     VRTWTREFGDRGIRFNTLSPGPVDTPIMDGQADSVEEADAIKAQFAAAVPLNRMGRPD
                     EIAAAALFLASDDSSFVAGAELSVDGGMAQV"
     gene            complement(4975..5379)
                     /locus_tag="VFC01_12805"
     CDS             complement(4975..5379)
                     /locus_tag="VFC01_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN80547.1"
                     /translation="MTVTDERNREFVLEAFDTLFNKRDYVAAERFWSSDYIQHSAHIE
                     PGRDGLFGLVKASPPDMRYENGLIVANGDYVMLHGRFSNVGQPADWIVVDVVRLQDGL
                     LAEHWDVIQDEATAEQSKSGLPMFGDIFPSTA"
     gene            complement(5428..6198)
                     /locus_tag="VFC01_12810"
     CDS             complement(5428..6198)
                     /locus_tag="VFC01_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN80548.1"
                     /translation="MASATVVQDLEGKVALVTGATSGIGKAAALQLAAQGSAVIVHGR
                     DVGRGAAVVAEIENAGGTARFVGADLGEPAEALRLAEEGGDVDILVNNAGFAWFGPSA
                     KLPVNTLDRLFTANVHAPYLLVSVLAPKMVARGDGVIINIASRAGTVGQPDTAAYGAT
                     KAALASLARSWAAEYGPAGIRVNAISPGPVYTNAADRDVFDASAETTLLGRAAEPQEI
                     ADLIGFLASPRAAYITGTNIAVDGGRAAARQHRSLDAA"
     gene            6389..6979
                     /locus_tag="VFC01_12815"
     CDS             6389..6979
                     /locus_tag="VFC01_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80549.1"
                     /translation="MATKTAKRPSARERLLAASDELFYRDGVHSTGIEAVIEKAGVAK
                     GSLYYIFGGKDELVAAYLRGRHEAWRQRVEAAQASAEDPDEKILAVFDAIADYVSLPE
                     FRGCPFENAAAEAPAGECQRQANKEYRDWLREYFLELAVATGVADSQALADALIVLYN
                     GALATANTAEPARTAGVTAKRIARLTLAAAKASSSA"
     gene            complement(7001..7462)
                     /locus_tag="VFC01_12820"
     CDS             complement(7001..7462)
                     /locus_tag="VFC01_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005241262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN80550.1"
                     /translation="MIENTGDFRALDPFFRIIEQGLAGYVDGEHFFDMLAEDVVFDFI
                     ITVPNYPRHVVGRANLIELYRGYGSTFFLDRCYDLRVHHSPSTSSVVLEYSSEGRVVA
                     TGQPYGNRYISVVVIKDRKITEWRDYLDPLRVFAALEGRPFEPDASAATDP"
     gene            7696..>8149
                     /locus_tag="VFC01_12825"
     CDS             7696..>8149
                     /locus_tag="VFC01_12825"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN80551.1"
                     /translation="MANRRSGSVARERLSRDSIVNGAIALADAEGLDAVTIRRLAQDH
                     EVSPMALYWHFKDKGELLDGIAERLFADVRLPAPSHNPWPEQLRSVLEALADALRPHP
                     EVAGLVSDRVLSSEAGLTLAEHTLALLRHAGLSAEQAAQVGGYLLNAVA"
BASE COUNT         1420 a         2543 c         2665 g         1521 t
ORIGIN      
        1 cgcggtgtcg catcggggca gggcgctggc cgcgctgttg ccggtgcttc gggagttggc
       61 tacaggccga acttctgctt gatgtcggcg gtctggaagt gctcgacgat gatgccgacg
      121 agcgggatgg tgcctgctag cagcacgccg atggtcttgc cgatgggcca gcgcaccttg
      181 acggcgaggt tggcggtgaa gatcaggtag acgaagtaga cccagccgtg gacgatcgcg
      241 atccagttcg gcgggttgtc gacctgcacg atgtacttca gcaccatctc gtagcagagg
      301 gcgatcagcc agaggccggt cgtccatgcc atcacgcggt agccgagcag ggccttgcga
      361 atggtttcga cgggggtggt cggggcttcg gtcatggggt cgggtgttcc ttgtcttgct
      421 tggcgaggtc ggcgaggtag gcgttgtact cgcggagtgt tgtgtcgtcg tcggaaggtg
      481 ttgcgggctt gggtctttcg ggcagtatcc cggaggggat ctcggtcacg gcgtcgtggt
      541 ttcgaggctc gggcggggcg tcctcgtagc ggacgaactt gtagtaggca tagaagcaga
      601 acccggcgaa cagcggccat tgcagggcgt aaccgaggtt ctggaaggtg cccgacgccg
      661 actcgaagcg ggtccactgc caccaggcca gggccaggca accggcggca gcgacgatga
      721 ccaacgcgat cagcgctggt ctcctacgcc gcgtagtgga caccgttcaa cggtaccgcg
      781 cggcccttcg ggccgataaa tgagtcgagg cgggcagtcg ccgcttttcg gtagatagag
      841 acgatgtact tggtcgaccg cgtccacggg atatcgaggg tgtcgagcgc ggcggtgaac
      901 aggccgatga tgtcttcgga tcggttggag aagtgatagc ggatgctcat gacgccgcgg
      961 tcgttcgcga cgacgcggca gccgtcgctg tggatcagcc cgaggatgaa ttcctcggtg
     1021 gcctggtcga cgagtacttg ctgccaaggt tccagcgcga tccgccgcat gtgcttcttg
     1081 ccggggccgt gctgcgggag caggcacggc caatgtttgg agtacagcga gacttcgacg
     1141 cagcctttcg gtaaagactg aaccgccgcc cgctgacgtg gcatcagcat gtcgatcgcc
     1201 tcgcggcatc gatcgatgat cgccggatac ttcttgtcca gcacaattct cagtcgccaa
     1261 actcggcggt cccgtgagat gcacccgtcg ccgaggtaca agcaagcagg tagcaatacg
     1321 gaatcgccga cagcgcggcg aagtcgtgaa taacgccgca gggcgttccg ccgtccaacc
     1381 tcggctggcg ctggggacgg caccgccagt cccggattgt cggccgtgga atgcccagtt
     1441 gacgtgcgat cgcgcaatcg ttaacaccgg ctgcgatgag tcgcttcgcg gcgtcgaact
     1501 cctctgctga ccgcatggcc tcaaactatg cggcgctgcc gacagatcgt gcacgtgagc
     1561 attaggtacg atcgaccacg ctgcgggcgt ggcgaaactg gtttacgcgc gggctttagg
     1621 tgcccgtgtt cgaaagaaca tgtgggttcg agtcccaccg cccgcacaac ttcgcaaagt
     1681 catggcaact cagcaagcgg cggggtgccc aaattggtgt cgatgagcca tgtgacatct
     1741 tcgagctgcg cccgattcgt gatcgcgtat gcatcccgga aatcggcagt tacccccgtc
     1801 ttccattgcc tacgcgcgaa attgaccaac acaaccgctc cgtacacgtc ggaaccggca
     1861 aggtcatggt cggccattcc cgggttgtct gacttgtcga gccactcttc tgtcatctgc
     1921 gcgatctcgc cgcgcgtcaa cctgggccaa cggcctcgtt gcaaggtccc tttgacctcg
     1981 atgctgaaaa cagcctcaaa gcctggtgtt gccatcagaa ggtcgacccc atgtccgcct
     2041 gactccggtc cgatcatttg ctggagcggg gtgtacccca aatcctccaa caatgaggcg
     2101 acaacggatt ccgcgatgtg tccggtgatc ccattcaacc ggctggtgcg atcggcgatc
     2161 gggatcgacg ccaacgaggt tgcgacagcg gttcgaaata actgttcgag ctcgcggatc
     2221 ttggtgtcgt acgcgatcag ttcaggtgac gcaccttcgt cttctctatt tgtccgggtc
     2281 gaatcggagc atgctatgac cagctcggcc ggcatttcga acggagtagc cgcgcgcaag
     2341 ctcgcccgca tcgcggtatg caaagctgga atgccctcgc cctcacaagc caatatgcct
     2401 agccttccag acagccccag tagtcagtat tgctgccggc ttagtgcttc cacggatgtg
     2461 tatgtccaga agtcgtcaac aaacaaatga tgacgacctc ggatagtggt attcctcgcg
     2521 aggctcagat ctctggtgtg cggtgggccc aaggcttcgc ctgctcgagc tgactcgcaa
     2581 cgcggatcag cacgtcttcg cgaccgtagg cggcgacgag ctgtacgccg accggcaagc
     2641 cctcggctgt ccactccagc ggcaggctga tcgctggctg gccgctggtg ttgaacgggg
     2701 gagtgaacgg cacgaaatcg ccggcgcgac gcatcggcga catcgggttg tcggggtcgt
     2761 tgacgatggt gccgagcttg atcggcacct cggcaactgt cggcgtgagg agcaggtccc
     2821 agccgtcgtg ccaccaggac tgaaccgccc ggcggtattg ggcgacggct gacaacgcga
     2881 gcccgtagtc gaccgcggtg atgcgtttgg caaagtcggc ctggacccgg ttcatcggct
     2941 ccaactctcg atcctccagg gggcgaccga gctgttcctc gaagcggcgc agcgacattc
     3001 ccatgttggc gctccacagc gcgccgaaca tagagccaaa cgtcttgtct cccaacgcct
     3061 tcggccacgc cggctcgacg tggtgaccca acgattcgag cagcttgccg acggcctgcg
     3121 ccgcatgggt gacttccgcg tcgacccgcc cgccctgcgg gtggtcgtcg aggatgccga
     3181 cgcgcagccg acccgggtcg gcgccgagct cctcgacgta cggacgtgaa ggcggtggcg
     3241 cgatcacggt gtcgccgatg cccgggccgt gtgtcgcgtc gagcaatgcg gccgtgtcac
     3301 gaacggtgcg gctgacgcag tgctcgaccg ccagtccgct ctcgtcgcgg agcgggccga
     3361 gcgtgatgcg accctgactc ggcttgagcc cgacaaggcc gcaacacgat gcagggatgc
     3421 ggatcgatcc gccgccgtca cttgcgtggg cgaatggcac catccccgac gcaaccgcgg
     3481 cggccgcacc gccgctcgat ccaccgggcg tgcgcgtcgt gtcccacggg ttgtgcgtag
     3541 caccccacgc gacaggctcg gtggtcggca cgctgccgag ctcggggcta ttcgtgcggc
     3601 cggcgatcac gagcccggcc gcgcggtagc ggctgacgag tgtcgtgtcg gcaccggcga
     3661 tgacatccgc ctccttgaac gcgacgttgc cgttgctgat ccgctgcccc gcgtaggtgg
     3721 cgaagagatc cttgatgagg aacggcacgc cgcggaacgg gccgtaggcg agatcggcgc
     3781 tcgcggcagt ctcccgcgcg tggtcgaacc agcggatcac gaccgcgttg agggccggat
     3841 cgatgcgttc aatgcgctcg atcgccgctt ccagcagctc gcccggcgac agttcccccg
     3901 acgcgacgag cgcagcttga tccctcgcat ccatccaacg tgtctgatct gcaagcgtgc
     3961 tcatggcagc ctgtgtagca cgaatcagcc acggctccgg cgggcccgcg tccctaactt
     4021 aggcgaatgt ctttgcgtgc gatggcgacg caagctcgtc atggacttct ggcaagacta
     4081 aacatcgtgc gcgtgacgac gctttacacg gcgctagacc tgcgccatgc cgccgtcgac
     4141 gctcagttcg gccccggcga caaagctgct gtcatcggag gccaagaaca gagccgccgc
     4201 ggcgatttcg tcagggcggc ccatacgatt gagtggtacg gccgcggcga actgtgcctt
     4261 gatggcgtcc gcttcctcca ccgagtcggc ttgaccgtcc atgatggggg tgtctaccgg
     4321 ccccgggctc aacgtgttga accggatgcc gcggtcgccg aactcccggg tccacgtccg
     4381 cacgtaggaa cgcagtgcgg ccttggtggc gctgtacgtg ctgtagccag gaatgcccat
     4441 gtagcccgcg atcgaaccga ccaggatgac cgagccggcg gtgcgcatca gaggtagtgc
     4501 cttctgcacc gtgaatagcg atccccgtgc gttgaggtcg aaggtgcggt cgaagttctc
     4561 ctcagtgatc ttccccaact cttctggctc cacccggccc gaattcacca cgaggatatc
     4621 cagcccgccc tcttcctcga ggaccgtcgc gaacagcttg tccaggtcgg cgccactcgt
     4681 gctatctatg gcgacggtag tcacgccgtc gccgatcaaa gccttggcct tgtccaactc
     4741 gcgctgtcga cgcccggtga tgaagacata cgcgccttcg tcgacgaacc tctgcgccgt
     4801 cgccagcccg ataccgctgc tgccgccggt gatcacggcg accttgccat tcagtcgtga
     4861 cattcgttaa tcctccaaat ttcgtggcga gtggtggacg tgcttgtgcg acgtccgttt
     4921 ctccggccac gctgtcgttg cgccggaaag tttgtggtgc gaaggcattc cgtgtcaggc
     4981 cgtgctcggg aagatgtcgc cgaacatggg cagcccgctc ttggactgct cggccgtcgc
     5041 ctcgtcttgg atgacgtccc agtgttcggc gagcaggccg tcctgtaggc gaacgacgtc
     5101 gacgacgatc cagtcggcag gttgaccgac attggagaag cggccgtgca gcatgacgta
     5161 gtcgccgttg gcgacgatca acccgttctc gtagcgcatg tctggcggcg aggccttcac
     5221 aagcccgaag agaccatctc ggcccggttc gatgtgagcg ctgtgctgga tgtagtcgga
     5281 ggaccagaac cgttccgcag caacataatc tcgcttgttg aacaacgtat cgaacgcctc
     5341 gaggacgaac tccctgttgc gctcatcggt aactgtcatg gccttgtctc ctgtgattcg
     5401 tgggacggga cgccagcatc gagatggtca tgccgcatct agactgcggt gctgtcgggc
     5461 ggcagcgcgg ccgccgtcga cggcgatatt ggtgccggtg atatacgcgg cgcggggcga
     5521 agccaggaag ccgatgaggt cggcgatctc ctgcggctcg gcggcccgtc cgagcagtgt
     5581 tgtctcggcg ctcgcatcga acacgtcgcg atcggctgcg ttggtgtaga cgggtcctgg
     5641 tgagatggcg ttgacccgga tcccggcagg cccgtattcg gcggcccagg agcgggcgag
     5701 ggaggccagt gcggctttgg tggcgccgta ggctgcggtg tcgggctgcc cgacggtgcc
     5761 cgccctgctg gcgatgttga tgatcacgcc gtcgccgcgg gccaccattt tcggggccag
     5821 gactgacacc agcaggtaag gggcgtgcac gttggcggta aacagccgat ccagcgtgtt
     5881 gaccggcaat tttgccgacg ggccgaacca ggcgaatccg gcgttgttga ccaggatgtc
     5941 gacgtcgccg ccctcttccg ccagccgtag cgcttctgcg ggttcgccca ggtcggcgcc
     6001 cacgaatcgt gctgtgcccc ccgcattttc gatctccgcg accaccgcgg cgccacgtcc
     6061 gacgtcgcgg ccgtgcacga tgaccgcgga cccctgcgcg gccagctgca atgcggcggc
     6121 cttaccgatc cccgacgtcg cgccggtcac cagcgcgacc ttgccttcca gatcctgaac
     6181 cacggtagcg cttgccatga ccactcctcg agtctttaga ccgatctgtc tgatctgtcc
     6241 aatttagacc ggtcagacta aagaagcaag tcttaggcaa atgttgcgtc tgagctgcat
     6301 ctgtgcgatg accgagcagc tgggaaatct gcagacgagg ccgatcggtg acgacgattc
     6361 agacaagtct gtctggtaga ctgacacgat ggcgacgaag acagcgaaac ggccgtcagc
     6421 acgggaacga ctgctggccg cgtccgatga gctgttctac cgcgacggtg tccactccac
     6481 cggaatcgag gccgtcatcg agaaggcggg tgtggccaaa ggctcgctgt actacatctt
     6541 cggcgggaaa gacgaactgg tcgccgccta cctacgtggc cgccacgaag catggcggca
     6601 gcgtgtcgag gcagcacagg ccagcgctga agatcctgac gagaagatcc tggctgtttt
     6661 cgatgcgatc gctgattatg tgtcgcttcc cgagttccgg ggctgtccct tcgagaacgc
     6721 cgctgccgaa gcgccggccg gcgagtgcca gaggcaggca aacaaggagt accgcgactg
     6781 gttgcgtgag tacttcctcg aactggccgt ggccaccggg gtggccgact cccaggcgct
     6841 ggccgacgcg ctcatcgtcc tctacaacgg ggcgctggcc accgccaaca cggctgagcc
     6901 cgcgcgcacc gcgggggtga ctgccaaacg catcgcgcgg ctcaccctgg ccgccgccaa
     6961 ggcctccagc tcggcctgat ccgagcttct ttcagcgatc tcatggatcg gtcgcggcgg
     7021 aggcgtcggg ctcgaagggg cgtccctcga gggcggcgaa gacgcgcagt ggatccaggt
     7081 agtcgcgcca ctcggtgatt ttgcggtctt tgatgacgac cacggagatg tatctgttgc
     7141 cgtacggctg accggtggcg acaacccttc cctcggagga gtattcgagc accaccgacg
     7201 acgttgacgg cgagtgatgg acgcgcaggt cgtagcagcg gtcaaggaag aacgttgagc
     7261 cgtagccgcg gtagagctcg atgaggttcg cgcggccgac gacgtggcgc gggtagttcg
     7321 gaaccgtgat gatgaagtcg aagaccacgt cctcggccag catgtcgaag aagtgctcac
     7381 cgtccacgta tcccgcaagg ccttgctcaa tgatccgaaa gaacggatcc agagctcgga
     7441 agtcgcccgt gttctcgatc actcgccgct cctcagaatc gttgacagca gacagttttg
     7501 gactgtgctg aggttctcac gcccgtcgtc ttcaatccca acgcagtgag ctgtctgttc
     7561 gctgctgtct cggatgcgat tgcaaccgcg gtgtctcctc gggggttcag tcccggggga
     7621 caacacggca gtcccatgct atagatcgat aggtgtacgg cgtacatgta gccatgtaca
     7681 agatatagtt gcagcatggc gaaccgtaga agcggatccg tggcccgcga gcggctgagt
     7741 cgcgattcca tcgtgaacgg ggccatcgcg ctggccgacg cggagggcct cgacgctgtg
     7801 accatccggc gactcgccca ggatcacgaa gtcagcccga tggcgttgta ctggcacttc
     7861 aaggacaagg gcgaacttct ggatggcatc gccgaacgcc tcttcgccga cgtccggctc
     7921 ccggcgccgt ctcacaaccc ctggcccgag caattgcggt ccgttctcga agcgctcgcg
     7981 gacgcgctgc gcccgcatcc cgaggtcgca ggcctcgtct ccgaccgcgt gttgtcctcg
     8041 gaggccgggc tgacactggc cgaacacaca ctggcgctgc tgcggcacgc ggggctttcc
     8101 gccgagcagg cggcccaggt cggcggatac ctgctgaacg cggtggcca
//
LOCUS       DASRJA010000293         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3989980,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000293 DASRJA010000000
VERSION     DASRJA010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3989980"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2248)
                     /locus_tag="VFC01_12830"
     CDS             complement(<1..2248)
                     /locus_tag="VFC01_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN80552.1"
                     /translation="MTVAASSAMGASSPYGRAPGLDNPALHSTQPYVQSGEIIVKFRD
                     RLPQDGAGKASGRPELDALMNRHAVTSIDRIARGRKASSPKAGQPDISRIYTIKYADG
                     TDAMQVAREFAAFPEVEYAEPRFIYQLDLIPNDALYASAQATYLNTRMKFTLAWDVTQ
                     GDDGTAVVAICDGGTHWNHEDLIAGIWNNSDEIPGNLMDDDNNGFVDDVRGWNFANNS
                     NDPTGLVATPNSANHGTHTAGIACATTNNALGIAGATYNAKYMPICVASPTTDNAIAF
                     GYDGIIYAAENDADAVNCSWGGQGGASAFEQDIINFAYNSGTVVVAAAGNNNTEAEHF
                     PSAYANTFAVANVTNTDIRNGSSNYGLWVDVSAQGTSIRSTLIGGYGNLTGTSMSAPF
                     AAAACALVRTRWPGYSTEQVMQRVRVTSDNIDGVNSSTYKGKLGYGRINAQQAMLKNT
                     PAISVTAFNYTTQDGDQVIEPGETITVNTTVTNWLAGATGCDFRLRDNSANIVVGDSI
                     QGLGSLDSLQSAVLPPFTFSVSPSAPIQHTVLFTLAITTTTPAYTDRSRFELTVLPVF
                     ATHDANLVDCSVTSVGKLGFALTAGGTGKDGIGWKYDGSNNLLFEGGLLIGTAAGSIS
                     DGARTMGSAQDDDFQTVPNGIPVVTEPATPFAEKGVATFTDALASVPIGINVKQQSYE
                     MLSPNEDFIILRYRIRNDSGLMRSNLHVGWYCDWDIDGGSFGTNRTAYDAGRGMIYAW
                     DDGAGPNAY"
     gene            2550..>2889
                     /locus_tag="VFC01_12835"
     CDS             2550..>2889
                     /locus_tag="VFC01_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80553.1"
                     /translation="MKNTPGSRKEKARDEMKQRGWVALGLGLVFFAGPAAGLAPKIGA
                     GAFGGASIPIVQDDNGTGPIYGIRVPVNLLQFLTAEPYFAHTGGGEAEETFGGIGYTR
                     SGFEINSFGLN"
BASE COUNT          473 a          870 c          992 g          554 t
ORIGIN      
        1 cataggcgtt ggggccggcg ccgtcatccc acgcgtagat catgccgcgg ccggcatcgt
       61 aggcggtccg gttggtgccg aaggagccgc catcgatgtc ccagtcgcag taccaaccga
      121 cgtgcaggtt gctgcgcatc aagccgctgt cgttgcggat gcggtatctc aagatgatga
      181 agtcctcgtt cggcgacagc atctcgtagc tttgctgctt gacgttgatg ccgatgggca
      241 ccgacgccag cgcatcggtg aaggtcgcga cacccttctc ggcgaagggg gtggccggct
      301 cggtgacgac cggaatcccg ttgggcacgg tttggaagtc gtcgtcctgt gccgatccca
      361 tggtccgagc cccatccgaa atgctgcctg ccgcagttcc gatcaggagc ccaccctcga
      421 acaacaagtt gttcgagccg tcgtacttcc aaccgatgcc gtccttgccg gtgccgcccg
      481 cggtcagggc gaagcccagc ttgccgacgc tggtgacgct gcaatcgacg aggttggcgt
      541 cgtgggtcgc gaacaccggc agtaccgtca gctcgaagcg gctgcggtcg gtataggccg
      601 gcgtcgtggt ggtgatggcg agcgtgaaca acaccgtgtg ctggatcggt gccgagggtg
      661 acacggagaa ggtgaacggc ggcaagacgg ccgactgcag cgagtcgagc gatcccaagc
      721 cttggatgct gtccccgacg acgatattcg ccgagttgtc gcgcagcctg aagtcgcacc
      781 cggtggcccc cgccagccag ttggtcacgg tcgtgttgac cgtgatcgtt tcgcctggct
      841 cgatcacttg gtcgccatct tgagtcgtgt aattgaaggc agtcaccgag atcgccggcg
      901 tgttcttgag catcgcttgc tgggcattga tgcgcccgta gccgagcttg cccttgtagg
      961 tcgacgagtt cacgccgtcg atgttgtcgg aggtgacgcg gacgcgttgc atgacctgct
     1021 cggtcgagta cccgggccaa cgggtcctga ccagcgcaca cgctgctgcc gcgaacggcg
     1081 ctgacatcga ggtgccggtc aggttgccgt agccgccgat cagcgtgcta cggatgctgg
     1141 tcccctgcgc cgacacgtcg acccacaagc catagttgct cgagccattg cgaatgtcgg
     1201 tattggtgac gttcgcgacc gcgaaggtgt tggcgtacgc cgagggaaag tgctccgcct
     1261 cggtgttgtt gttgcccgcc gcggcgacga ccacggtgcc gctgttgtac gcgaagttga
     1321 tgatgtcctg ctcgaacgcc gaggcgcctc cctggccgcc ccagctgcaa ttcaccgcgt
     1381 ccgcgtcgtt ctccgccgcg tagatgatgc cgtcgtaccc gaaggcgatg gcgttgtcgg
     1441 tggtcggcga ggcgacgcag atgggcatgt acttggcgtt gtaggtcgcg ccggcgatgc
     1501 cgagggcgtt gttggtggtg gcgcaagcga tccccgcggt gtgagtcccg tggttggcgc
     1561 tgttcggggt cgccaccagg ccggtcggat cgttgctgtt gttggcgaag ttccaaccgc
     1621 ggacgtcgtc gacgaagccg ttgttgtcat cgtccatcaa gttgccgggg atttcgtcgg
     1681 agttgttcca gatgcccgca atcaagtctt cgtgattcca atgggtcccg ccgtcgcaga
     1741 tggcgaccac cgcggtgccg tcgtcgccct gggtcacgtc ccacgccaag gtgaacttca
     1801 tcctggtatt gaggtaagtg gcctgggcgg aggcgtacag cgcatcgttc gggatcaggt
     1861 ccagctggta gatgaatcgc ggttcggcgt attcgacctc ggggaaggcg gcgaactcgc
     1921 gcgccacctg catggcatcc gtgccgtccg cgtacttgat cgtgtagatg cgcgagatgt
     1981 ccggctggcc ggccttgggc gaactggcct tccggccgcg cgcgatgcga tcgatcgagg
     2041 tcacggcgtg ccggttcatg agagcgtcga gttccgggcg accgctggcc ttgccggcgc
     2101 cgtcctgcgg caaccggtca cggaacttga cgatgatttc gccggactgg acgtatggct
     2161 gggtcgagtg caacgccggg ttgtcgagcc cgggtgcgcg accatagggc gaactggcac
     2221 ccatggccga actggcggca acggtcatcc aagtcgcacc gaccaaagcg ggtgcgagac
     2281 gacgccgaac acgcatgatt ccccctcgtt tgcagaagtg ggcagaagac gcagtaggaa
     2341 acgaggcgag cccggtggca gcccaaaggg acggctgcgg ctgcttgggt gacgcgcctg
     2401 aatctggaaa agtgtagacg tcggaggctg ggaactgcaa gttcgcaagc ctagctgccg
     2461 ctcgattcgg tccggggtcg gccctctggg ggccgaagca ccgagacgta gtcgcgggga
     2521 ctccctccgg gcacggcccg tcccgttggt tgaagaacac tcccggcagt cggaaggaaa
     2581 aggcaaggga tgaaatgaag caacgagggt gggttgcgct cggcctgggg ctcgtgttct
     2641 ttgcgggtcc ggctgctggc cttgcaccca agatcggcgc cggcgccttt ggcggtgcca
     2701 gcatcccgat cgttcaggac gacaacggca cggggccgat ctacggaatc cgcgttccgg
     2761 tgaatcttct ccagttcctc accgcggaac cctatttcgc ccacaccggc ggcggcgagg
     2821 ccgaggagac tttcgggggc atcggctaca cccgatcggg cttcgagatc aactccttcg
     2881 ggctcaacg
//
LOCUS       DASRJA010000294         2991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3226481,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000294 DASRJA010000000
VERSION     DASRJA010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3226481"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFC01_12840"
     CDS             complement(<1..280)
                     /locus_tag="VFC01_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80554.1"
                     /translation="MTDTVVLRFVFRVGANEATGAQAGDENEYAIRKSIRVFRHVDPD
                     QVCRVVWSDRGPPHDRHSFLTAELAITGAAQLRILAEGHPFTPDMLPVI"
     gene            270..1649
                     /locus_tag="VFC01_12845"
     CDS             270..1649
                     /locus_tag="VFC01_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding monooxygenase"
                     /protein_id="HZN80555.1"
                     /translation="MSVKNRSGHAVVLGSSIAGLLAARVLSESFEKVTVFDRDALPEG
                     HDNRRGVPHGEHTHGVLAFGRAALEELFDDFAADITALGALPLDLGRDLIWFGDGMRF
                     PRQDTGLLGLGVSRPTLEGYLRDRVRRLSNVVIQDRTEALGLLTSEDRRQVTGARVLP
                     RGGQPLEIPADLVVDATGRGNRGPTWLSEIGYAKPVEEAVDAVAGYSTRVYRRVPGDA
                     DFAGVVLNPWPHNPQGAVAVAVDGVRWMVTLVGYESTNFPPTGPDAFVDFLRRLPPPE
                     LYELLSRAEPLGPPLKMRLPVSVRRRYERMRDLPEGFISIGDAICAFNPAYGQGMTVA
                     AAEALVLRDCLVKGSKGLPGRFYAKVAKLINVPWDMAVGADLRYGHVIGARSGKTAFL
                     NGYIARLFVAASRHPAVAKAFLSVANLMSPPQRLFAPPVLARVLAYGRKPQAATAAAE
                     PVRTLQTIG"
     gene            complement(1742..2989)
                     /locus_tag="VFC01_12850"
     CDS             complement(1742..2989)
                     /locus_tag="VFC01_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HZN80556.1"
                     /translation="MATEKWVTRTAAALGGAALVLALGISDAEAAFFSGETGVDWNVV
                     FLLLGMMIIVGVMRETGVFEYLAIWAAKRARGRPFPLMVLLILITAGASALLDNVTTV
                     LLIAPVSILLCRRLGLPVVPYLLAEVFASNIGGTATLVGDPPNIIIGSRGGLTYNDFL
                     LHLTPIVTLLLIVFVGLCRWLFRDAFRHDPQRAASVMALRERDAINDGRLLIVSGAVM
                     ALVTAAFVLHTVLHLEPSVVAIVGGLLLLAASRLDPKDVAKDVEWPTLVFFTGLFVMV
                     GGLVATGVIDRIAHAAADAVQGKLFLTSMVLLWGSAVLSAIVDNIPYVATMSPIVAEL
                     VAANGGGPDSQVLWWSLALGGDLGGNATAIGASANVVILGMAERAGRRISFWEFTKYG
                     LVVTAITVALCVPYLWLRYFAFG"
BASE COUNT          514 a          960 c         1052 g          465 t
ORIGIN      
        1 gaatgacggg gagcatgtct ggtgtgaacg gatgaccttc ggccaggata cgaagctgcg
       61 cggcgccggt gatggcgagc tccgctgtta agaaggaatg gcgatcgtgc ggtggtcccc
      121 gatccgacca gaccacgcgg catacctggt cagggtcaac gtgacggaac acacgtatcg
      181 actttcggat cgcatattca ttttcatcac cggcttgggc gcccgtagct tcgttggcgc
      241 caaccctaaa gacgaaacgg agaacaaccg tgtctgtcaa gaaccgatcc gggcacgccg
      301 tcgtgctcgg atcgagcatc gccggcttac tggccgcgcg cgtgctcagc gagtcgttcg
      361 agaaggtcac cgtcttcgac cgcgacgcgc ttcccgaagg ccacgacaac cgccgcggcg
      421 tgccgcatgg ggagcacacg cacggcgtgc tcgccttcgg acgcgcggcg ttggaggaac
      481 tcttcgacga cttcgccgcg gacatcaccg cgctgggcgc gttgccactc gatctcggcc
      541 gcgacctgat ctggttcggt gatggcatgc ggtttccgcg gcaggacacc ggactgctcg
      601 gccttggcgt aagccggccg acgctcgaag gctatctacg cgaccgtgtc cgccggctgt
      661 ccaatgtggt tattcaggat cgtaccgagg cgctggggct gctaaccagt gaggaccgcc
      721 gccaggtgac cggcgcgcgg gttctgcctc gtggcgggca gccgttggag atccccgcgg
      781 atctcgtcgt cgacgccacg ggccggggaa accgcgggcc aacctggctc tcagaaatcg
      841 gctacgccaa gccggtcgag gaggcagtgg acgccgtcgc gggctattcg acccgcgtgt
      901 atcgccgggt gcccggcgat gccgacttcg caggtgtcgt actcaacccg tggccgcaca
      961 acccgcaagg cgccgtggcc gtggccgtcg acggggttcg ttggatggtg acactcgtag
     1021 ggtacgagag caccaacttc ccgcctacgg gtccggacgc gttcgtggac ttcctcaggc
     1081 gcctgccgcc gcccgaactc tatgagctgc tgagccgggc cgagccgctg ggcccgccgc
     1141 taaagatgcg gctcccggta agcgtgcgcc gccgctacga gcggatgcgg gacctgccgg
     1201 aaggcttcat ctccatcggc gatgccatct gcgcattcaa ccccgcctac gggcagggaa
     1261 tgaccgttgc cgccgccgag gcgctcgtgc tgcgcgactg cctggtcaag ggaagcaaag
     1321 ggctgccagg ccgcttctac gccaaggtgg ccaagctcat caacgtcccg tgggacatgg
     1381 ccgtcggcgc cgatctgcgc tatgggcacg tgatcggtgc ccggtcgggc aagaccgctt
     1441 ttctcaacgg ctacatcgcc cggctgttcg tcgctgcgtc ccggcatccg gcggtggcca
     1501 aagccttcct gtcggtggcg aacctgatgt cgccaccgca gaggctgttc gcaccgcccg
     1561 tcctggcacg ggtgctcgcc tacggccgca agccacaggc ggctacggct gccgccgagc
     1621 ctgtgcgcac gcttcagacc atcgggtgac ccgacggcgt gatgggccgc caccttggga
     1681 cggcggccca tcaccaaggc cgcgaccagg ttgaggcggg tcgaggtctc ctgcggggcg
     1741 gctatccgaa ggcgaagtag cgcagccaca ggtatggcac gcacaaggcg acggtgatcg
     1801 cggtgacgac gaggccgtat ttggtgaact cccagaagct gatgcgtcgt ccggcgcgtt
     1861 cggccatgcc gaggatgacg acgttggcgg aggcgccgat ggcggtggcg ttgccgccga
     1921 ggtcgccgcc gagggcgagg gaccaccaca gcacttggga gtcagggccg ccgccgttgg
     1981 cggcgacgag ttcggcgacg atggggctca tggtggccac gtaagggatg ttgtcgacga
     2041 tggccgacag cacggcggag ccccataaca ggaccatcga ggtgaggaac agcttgcctt
     2101 gtacggcgtc ggcggctgcg tgggcgatgc ggtcgatgac gccggtggcg accaagccgc
     2161 cgaccatgac gaacaggccg gtgaagaaga cgagagtggg ccattcgacg tctttggcga
     2221 cgtccttggg gtcgagtcgt gatgcggcca gcagcagcaa cccgccgacg atggcgacca
     2281 ccgatggttc gaggtgcagc acggtgtgca ggacgaacgc ggcggttacc agcgccatga
     2341 ccgcgccgct gacgatgagc agccggccgt cgttgatggc gtcgcgctcg cgcagagcca
     2401 tgacggaggc ggcccgctgc gggtcgtggc ggaacgcgtc gcggaacaac catcggcaca
     2461 gtccgacgaa cacgatcagc agcagtgtca cgatcggggt caggtgcagg aggaagtcgt
     2521 tgtaggtcag gccgccgcgg ctgccgatga tgatgttcgg cgggtcgccg accagggtgg
     2581 ctgtgccgcc gatgttcgag gcgaacacct cggccagcag gtagggcacc accgggagcc
     2641 ccagccgcct gcacagcagg atggagaccg gtgcgatcaa cagcaccgtg gtgacgttgt
     2701 ccagcagcgc cgacgcgccc gcggtgatca ggatcagcag aaccatgagg gggaagggcc
     2761 gcccgcgggc ccgcttggcc gcccagatcg ccaggtactc gaagaccccg gtctcgcgca
     2821 tcacgccgac gatgatcatc atgccgagga gcaggaagac gacgttccag tcgacgccgg
     2881 tctcgccgga gaagaaggcc gcttcagcgt cgctgatccc gagcgccagc accagtgcgg
     2941 ccccacccaa tgccgccgcg gtgcgggtga cccatttctc ggtggcgatg a
//
LOCUS       DASRJA010000295        11623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4010768,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000295 DASRJA010000000
VERSION     DASRJA010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4010768"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..735
                     /locus_tag="VFC01_12855"
                     /pseudo
     CDS             1..735
                     /locus_tag="VFC01_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892473.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
     gene            complement(1213..2085)
                     /locus_tag="VFC01_12860"
     CDS             complement(1213..2085)
                     /locus_tag="VFC01_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY domain-containing protein"
                     /protein_id="HZN80557.1"
                     /translation="MRTTIAALCLVAVAIAPACSRNKPPNSLFDGAGYHVREDKVYYL
                     TAFPGKASEIAGADAASFKAFDTTYAKDNFNAYFDGSPISGADATTFDVLDRDGFAKD
                     RNHVYQLDRPISDDPVHFELLEGGLSKDSSAVYWTDGDVLSDDPAHFAIVSNTDHYLF
                     TKDSRTVHVNGDPVADADPATFRVLHEAYAQDATHVFYFSDPIVGADAGSFRALAGPY
                     ASDIRNVYWMGKVIDGADPVTFRVLNAPFECSADAGRAYYRQTVIANAEPRTFPPGRE
                     VTNCSETSISFAES"
     gene            complement(2102..2758)
                     /locus_tag="VFC01_12865"
     CDS             complement(2102..2758)
                     /locus_tag="VFC01_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HZN80558.1"
                     /translation="MCIRIAAFVTALCTAIGAAGTAWGSPAVPPVSDAARGAGLVDVR
                     TVVPDAVVDLRYATPNNFVGVPLYPADARCLVHESMAPGLAAAAAVLRPRGERLVFWD
                     CYRPHDVQVRMFEAVPDPNWVARPGPYSRSHEAGRSVDVTIANDQGLVDMGTDFDDFT
                     PRALAYATEGVSPAAQANRARLRDAMNAGGLTVYKGEWWHFDTTDAYEKRPILNAGVN
                     "
     gene            2849..4078
                     /locus_tag="VFC01_12870"
     CDS             2849..4078
                     /locus_tag="VFC01_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN80559.1"
                     /translation="MSAATVTSPTVFEADLPSVSYEDAPTPEAAHAALKQAREESPIA
                     MGAHGPEILRYELAHTALRDHRMSPPPGMGLEAQGITSGPLWDRVSASLLSVNGEDHA
                     RLRRLVSKAFTPRAVARLDTTITDIITQLTDPLMAVGSSEIVADIARPYPVPVICELL
                     GAPRQDWQLFSEWADEFFKTFTWKAAEYEPQILAAWGELDDYVDAMVAERRNTPTDDL
                     ISELIRAEDEGDRLTMAELRMLAGGILMAGTDTTRNQVAAAIDVLCDHPDQWDMLAEH
                     PELAMKAVEELMRVYPVVFGVMRMTTEDVEYEGIVIPADTFVWVNTAAANRDPAVYDD
                     PDRLDITREGAPPMQSFGAGAHYCLGANLARREIAEALIVMARRMRNLRRAGPAPWKP
                     LVAISGPAELPVEFDEA"
     gene            4132..5250
                     /locus_tag="VFC01_12875"
     CDS             4132..5250
                     /locus_tag="VFC01_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HZN80560.1"
                     /translation="MRDVLDELLTVWQAGETAGVATVVRTIKSAPRPPGATMVVAPNG
                     TVAGSVSGGCVEAAVYELANEVVASGRPELQRYGISDDDAFAVGLTCGGIIDIFAEPV
                     SRNTFPQLQSIADDIAAHRPTAIATVIAHPDSDWLGRRLVIGLHAVEGSLGSERADAA
                     VADDARGLLAAGRTTVLSYGSDGQRQETGMEVFVASHAPRPRMLIFGAIDFASALARQ
                     GAFLGYRVTVCDARPVFATPARFPDADEVVADWPDRYLTAQAEQGAIDSRTAICVLTH
                     DPKFDVPALQVALRLPEVDYIGVMGSRRTHDDRMNRLREVGLTDAELSRLFSPIGLDL
                     GARTPEETAVSIAAEIIARRWGGGGRPLTEVNGRIHHD"
     gene            5330..7654
                     /locus_tag="VFC01_12880"
     CDS             5330..7654
                     /locus_tag="VFC01_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZN80561.1"
                     /translation="MTDTVATRYAGTRVPRIEDNRLLTGRGTFVDDVSRPGMLHACFV
                     RSPFAHAKIKAIDAGAALALPGVHAVFTANDLNPDVKEAWHAVAGKDIPDTPRPPLAE
                     GEVKFVGDPLALVVAESRYIAEDAVEMVDVDYEPLPAIADFTRAAGADVVVHEAYPDN
                     VAGGLSGAPPDEETFSSAAHVAEAHVYQQIYAPVPMETRGMVVEWEASSEELTVWAST
                     QTPHELRAFAARLLGIPAQGVRVIMRDTGGGFGQKVVPMREDMCILLAARKVPFALKW
                     IEDRRENLMSAGQARHVDGKARMAFDDEGNILAADIDFIYDIGAYPTPYPVLTTAAVG
                     MFFPGPYRVPKASFNYKTVFSNTAGLAAYRGPWQYETLTREILLDIAARKIGMDPVEL
                     RRRNILRGDEMPYFNPNGMPYDHVAPADSLEQAVKILDHEGFRREQREALEQGRYLGL
                     GFSAYIEPTGAATGHLATEGATIRMEPTGKINVYVNGGSTGNSLETTVVQLTADALGA
                     NIDDVSTIQGDTAVTPYGAGTQGSRSGPMTAGAVNEAGTMLRKQIVAMAAARLGVDED
                     AIELGGSVASVREDPSKSVTFADIAYRSYYEPQQLPPGMPPTLEATARFTSQAPIHWA
                     NATHACTCEVDVTTGQVTLTRYIVSEDVGAMINPNVVEGQIAGGTVQGIGGALLENMV
                     YDDDGNPLASTFVDYLLPTATDVPPIEYGHIEIPGPGVGGYKGVGEGGAIGSTPAVIN
                     AINDALAPLGVTLTRLPASPAAIVELIEQARKDH"
     gene            7657..8286
                     /locus_tag="VFC01_12885"
     CDS             7657..8286
                     /locus_tag="VFC01_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN80562.1"
                     /translation="MELNNEFRVAVPAAKTWEFLTDVERVAPCLPGATLLSVDGDEFT
                     GAVKVKVGPITVSYKGEAAFQEKDATAHRMVLRANGKETRGNGNAAAVVTAQLKDEGD
                     ATSVVITTDLTISGKAAQFGRGVLADVAGNLIGQFAKALEADLLGGAPAAQTTSTASE
                     PTAGAAAGDSVDLLKVVAMPMAKRAAPALVALVAGAAIGFLLGRRNRQS"
     gene            8283..9158
                     /locus_tag="VFC01_12890"
     CDS             8283..9158
                     /locus_tag="VFC01_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZN80563.1"
                     /translation="MKAAAFAYHRPDAVEEAVDLLAEFGDDAKILAGGQSLVPMLAMR
                     LTHFDNLVDISRIDELVGIDLCDGEIRIGAATPHAFVGMDDEVADSVPLLTLATPYIG
                     HFQIRTRGTLGGAIAHADPAAEYAAVALALDAQMEAVSPRGTRDIPAKEFFTGLWENS
                     MATDEILTAVRFPVWGARSGFAVEEFARRHGDFAIAGAAVAVELDDDSRVSRCGIGLL
                     GLGSTPLRGSPAEEAVIGRSVDEITADEIGQLAISALAEIPADLQGSATYRAGVGATM
                     VARAWKSATAEASNA"
     gene            9151..9636
                     /locus_tag="VFC01_12895"
     CDS             9151..9636
                     /locus_tag="VFC01_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN80564.1"
                     /translation="MHEWPVQVSVNGRPTQASVEPRLTLADYLRERCGLTGTHLGCEH
                     GACGACTVLVDGQAVRSCLMFAVQADGCEVTTVEGIASEDGELSPVQSALRECHGLQC
                     GFCTPGFVVSITALLRDNPKPTDEEIREGLSGNFCRCTGYQGIVNAVRRAAEVNAEVL
                     G"
     gene            9860..10189
                     /locus_tag="VFC01_12900"
     CDS             9860..10189
                     /locus_tag="VFC01_12900"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN80565.1"
                     /translation="MTAKLQQAAQAPAPAQQAAPGPSGQQTDEGFLNALQKRTVPIIS
                     DSAAIDLAHSTCALLERGGTAQEAVSHVGRSDALRHYPNSQDRELKNLQAFTSIAVGA
                     YCPQMTQ"
     gene            10220..11116
                     /locus_tag="VFC01_12905"
     CDS             10220..11116
                     /locus_tag="VFC01_12905"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80566.1"
                     /translation="MKIGFIGLGNMGAGMAANLLKAGHEVTAYNRSQDKVAALAKQGA
                     KPAKSVAETCGGDVVVTMLANDDAVAAVAFGDHGIIASLRPGATHVSSSTISVALSER
                     LTAAHAEAGQQFVAAPVFGRPEAAAAAKLFVVAAGAVAALQPLSPVFDAIGQRTFVVS
                     EEPTAANLVKLSGNFLIASVIESLGEAMALVAKAGVDKNDYLEILTSTLFGAPIYKTY
                     GGLIAREEFEPAGFAAELGLKDIRLVLAAGETLQTPLPIASLLRDRFLTLLANGGGSL
                     DWSAVGALPAWEAGGSSPTARD"
     gene            11163..>11623
                     /locus_tag="VFC01_12910"
     CDS             11163..>11623
                     /locus_tag="VFC01_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80567.1"
                     /translation="MDREDPEFRIADERQGQRRRHRSGGPGKLDLGNAFWWVVALIGP
                     VVGAAWVLTLALPSSALWMSRIVCSGSHNMEHRGPHFECVSDAGSHTVNILAVVGAPS
                     LAHRARAVRRRGSQRRGSAAAAEAHVTVPLRTVVGPLHPAGAVVMGNRRRP"
BASE COUNT         2058 a         3777 c         3904 g         1884 t
ORIGIN      
        1 cagttctccc cgttgtccgt cgagtatgcg agcgccgagt aattggtcgt ccagtggggt
       61 gcgcccggcc agtggacgac caattactcg gcgctcgcat actcgacgga caacggggag
      121 aactggaccg tcgctccttc aaccgtccga tacaaccagc cctggagcgg taaccaaaac
      181 ttccagcagt cggcgttcgt ccgacccggc gatggatacg tctacatgta cggcacgccg
      241 aacggccgcc aaggcgccgc gtacctgtcg cgcgtccccg agcgggacat cctcaacctc
      301 agcaggtacg agtactacag caagggcact gccggcgggt ggttcggatg gggcgcgacg
      361 ccggcagggt ggtacaggaa cgatcccagc aaggccaccc ctgtcttcgg gcaggacacg
      421 ggcgcctgcg gcgttgccaa cgccggcaac caagtcagcg agatgtcggt tcagtacaac
      481 aagtatctga agaagtacgt cgtgctctac ggagatcagt tcaacaacat cgtgatgcgc
      541 acgtcggaca cgccgcaggg cacgtggtca gccgcaaagg tgttgatgcc ccagcagacc
      601 ggcggaatct acgcgccgat gatgcacccg tggtcgccgt cgaccatggg caccggtagc
      661 gacctgtact ggaacctgtc gttgtgggat cgctacaacg tcatgttgat gcgcactgac
      721 ctcaccaagg tctaggccgg tgcgcaaagt cgcgatagcg gtcagcacgc tgacgggcgc
      781 gctgttgacg gcggccgtcg cctccgcaga tcctcctgcg ccgcaagctg atacaccctg
      841 ctcatcggat gtcgcgaatg taatgacctg gccggcgaat gcgaagatgc cgctggtatg
      901 ccacaacggt caatggcaga ccgtgacgtc tcccccgcca ccgaacgacc gatggttgag
      961 cgtcgggccc gccatgacgc tgcacggaga aggccagcga aatcccagtg tcgcatcggg
     1021 cgactggacc gcggtaccac agaatgcgaa cagcagttgt cgcgccgagc agcgagcggt
     1081 tctcgaaccg ggcgtcaccg ctccgccaca ggtcgcggaa agtcctcaag gacaaccgct
     1141 ttcattccag gtggtgccgc ggctcttctc gatagagatg agcgggtact gcctgtggac
     1201 gcggaggaat agctaggact ccgcaaacga gatcgatgtt tcgctgcagt tcgtgacctc
     1261 acggcccggc gggaatgtgc gcggctccgc gttggcgatg acggtttgcc ggtagtaggc
     1321 gcgcccggcg tcggctgaac actcgaaggg cgcattcagc acgcggaacg tgaccgggtc
     1381 ggcaccgtcg atcaccttgc ccatccagta gacgttgcgg atgtcgcttg catacggccc
     1441 tgcgagcgca cgaaacgatc ccgcgtcggc gccgacgatc gggtcggaga aatagaagac
     1501 gtgcgtagcg tcctgcgcgt aggcctcatg cagcacccgg aacgtcgccg gatccgcgtc
     1561 ggcaactggg tctccgttga cgtgcaccgt tcgactgtcc ttggtgaaca ggtagtggtc
     1621 ggtgttcgag acgatcgcga aatgcgcggg atcgtctgac agcacatcgc cgtcggtcca
     1681 gtacacggcg gagctgtcct tcgacagacc gccttcgagc agctcgaagt ggaccggatc
     1741 gtcgctgatc ggtcggtcga gttggtagac gtgattgcgg tccttcgcaa agccgtcgcg
     1801 gtcgagcacg tcgaaagtcg ttgcgtcggc gccagatatc gggctgccgt cgaagtatgc
     1861 gttgaagttg tccttcgcgt aggtggtgtc aaacgccttg aaggacgcgg catctgcgcc
     1921 tgctatttcg gatgccttgc cggggaaagc ggtcaggtag tagaccttgt cttcgcgcac
     1981 gtggtagccc gcgccgtcga acagcgagtt gggcggcttg ttacgactgc aggccggcgc
     2041 gatggccacc gcgacgaggc acaaggccgc gatcgttgtc cgcatccgac gatcgaacca
     2101 cctaattcac acccgcattg agaatcgggc gcttctcgta cgcgtcggtg gtgtcgaagt
     2161 gccaccactc gcccttgtat accgtcagtc cgcccgcgtt catcgcatcc cgcagtcgcg
     2221 cacggttggc ctgcgcggcg gggctgacgc cctcggtcgc gtaggcgagg gcgcgcgggg
     2281 tgaagtcgtc gaagtccgtg cccatgtcga cgagtccctg atcgttggcg atcgtcacgt
     2341 cgacggatcg gccggcttcg tggctgcgcg agtagggccc gggccgcgcc acccagttcg
     2401 gatcgggcac cgcctcgaac attcgtacct gcacgtcatg cggccgatag cagtcccaga
     2461 acacaagccg ttcgcccctc ggccgcagca cggcagcggc ggcggcgagc ccgggcgcca
     2521 tcgattcgtg caccaggcag cgggcgtcgg cgggatacag cggcacgccg acgaagttgt
     2581 tcggggtcgc gtaccgcaga tcgacgaccg cgtcgggcac gacggtccgc acgtcaacca
     2641 gtcccgcacc ccgcgcggcg tccgataccg gaggcaccgc cggcgacccc catgcagtgc
     2701 cggcagcacc gatggcagtg cacagtgcgg tcacgaaagc cgcgatgcgt atgcacatgc
     2761 aaacacctcc tgaaaagcga gtacgtatct acgctatcgg cctggatcgc gcggccagac
     2821 gcttaaggcg tcccgaaagg aggccagcat gagcgccgcc accgtcacat ccccgaccgt
     2881 tttcgaagcc gacctgccct ctgtttccta cgaggacgcg cccaccccag aggccgcgca
     2941 cgcggccctc aagcaagcgc gcgaagaatc cccgatcgcc atgggcgcgc acgggcccga
     3001 aatcttgaga tacgagctgg cgcacaccgc gttgcgtgat caccggatgt ccccgccacc
     3061 cgggatgggc ctcgaggccc aaggcatcac gtcggggccg ttgtgggaca gggtgagcgc
     3121 gtcacttctg agtgtcaacg gcgaggacca cgctcgtctg cgccgactgg tgtccaaggc
     3181 attcacgccg cgagcggtcg cccggctgga cacgacgatc accgacatca tcacgcagtt
     3241 gaccgatcca ctgatggccg tcggaagctc tgagatcgtc gccgacatcg cgcgcccata
     3301 tccggttccg gtcatctgcg aactgctcgg cgcccctcga caggactggc agctgttctc
     3361 ggaatgggcc gacgagttct tcaagacctt cacctggaag gccgccgagt acgaacccca
     3421 aatactggcg gcctggggcg aactcgacga ctacgtcgac gcgatggtcg ccgagcgccg
     3481 caatacgccc acggacgacc tgatctcgga actgatccga gccgaggacg agggcgaccg
     3541 cctgacgatg gccgaactac gcatgcttgc cgggggcatc ctcatggcgg gcaccgacac
     3601 cacccgcaac caggtggccg ccgccattga tgtgctctgc gatcacccgg atcagtggga
     3661 catgctcgcc gagcatcccg agctggcgat gaaggcagtc gaagagctga tgcgcgtcta
     3721 cccggtggtc ttcggtgtga tgcggatgac gaccgaagac gtcgaatacg agggcatcgt
     3781 catcccggca gacacattcg tctgggtcaa caccgccgcc gccaatcgcg accccgcggt
     3841 atacgacgac ccggatcggc tcgacatcac gcgcgagggc gcgccgccga tgcagtcatt
     3901 cggcgcgggt gcgcactact gcctcggcgc gaacctcgct cgacgcgaga tcgccgaagc
     3961 cctgatcgtg atggcccggc ggatgcgcaa cctgcgtcgc gcaggccccg caccgtggaa
     4021 gccgttggtg gccatcagcg gtcctgcaga gttgcctgtc gagttcgacg aggcctgact
     4081 caacgctaag cactcgcaca gccgacggcg ccatgtgcct agactttgaa tgtgcgtgac
     4141 gtcctggacg agctgctgac ggtctggcag gccggcgaga cagcgggcgt cgccaccgtc
     4201 gttcgcacga tcaagtccgc gccgcgtccg cccggcgcca ccatggtcgt cgctcccaac
     4261 ggcacggtcg cgggttcggt gtccggtggc tgcgtcgagg cggctgtcta cgaactggcc
     4321 aacgaggtgg tcgcaagcgg gcgacccgaa ctgcagcgct acgggatatc cgacgacgat
     4381 gccttcgccg tcggactcac ctgcggcggg atcatcgaca tcttcgcaga gccggtgtcc
     4441 cgcaacactt ttccgcagct gcagagcatc gccgacgaca tcgcagcgca tcgcccgacc
     4501 gcgatcgcca cggtcatcgc gcatcccgac tccgattggc tcggacgcag gctggtcatc
     4561 ggcctgcacg ccgtcgaggg gtcactggga agtgagcgtg ccgacgccgc cgtcgccgac
     4621 gacgctcggg ggctgctcgc cgcgggtcgc accacggtgc tcagctacgg ctccgacggc
     4681 cagcgtcagg aaaccggcat ggaggtgttc gtcgcgagcc acgcaccgcg gccgcggatg
     4741 ctgatcttcg gcgcgatcga cttcgcctcg gccctcgccc ggcagggcgc gtttctcggc
     4801 taccgggtca ccgtttgcga cgcgaggccc gtcttcgcga cgccggcccg cttcccggac
     4861 gccgacgagg tcgtcgccga ctggcccgac cgctacctca ccgcgcaggc cgagcagggc
     4921 gcgatcgact cccggaccgc gatctgcgtg ctgacccacg accccaagtt cgatgttccg
     4981 gcgctgcagg tcgcgctgcg gttgcccgag gtcgactaca tcggggtgat gggatcgcgg
     5041 cgcacccacg acgaccggat gaaccggctg cgcgaggtcg gcctgaccga cgccgagctg
     5101 agccggctgt tcagcccgat cggcctcgac ctgggcgcgc gcacgcccga ggagaccgcg
     5161 gtgtcgatcg ctgcggagat catcgcgcgt cgctggggcg gcggcggcag gccattgacc
     5221 gaggtcaacg gccgtatcca ccacgattga ttagatgaga caaatgaggt ggaaatgtcg
     5281 ctctcagatg cgataacgtg actctcagcc gttcgagggg agtgatggcg tgacagatac
     5341 ggttgccacc cgatacgcgg gcacccgcgt ccctcgcatc gaggacaacc gccttctcac
     5401 cggccgaggc accttcgtcg acgacgtttc caggcccggc atgctgcacg cctgcttcgt
     5461 gcgcagcccg ttcgcgcacg cgaagatcaa ggccatcgat gcgggcgcgg ccctggccct
     5521 gcccggggtg cacgccgtgt tcacggcgaa cgatttgaac ccggacgtca aggaggcctg
     5581 gcacgcggtc gcgggcaagg acatcccgga cacgccacgc cccccgctag ccgagggtga
     5641 ggtgaagttc gtcggcgacc cccttgcgct cgtcgtcgcc gagagccggt acatcgccga
     5701 ggacgcggtc gaaatggtcg atgtcgatta cgaaccgctg cccgccatcg ccgacttcac
     5761 ccgagctgcc ggcgctgatg tggtcgtcca tgaggcgtat ccggacaacg tcgcaggcgg
     5821 actgagtggc gcgccgcccg acgaggagac cttctcctcg gccgcgcatg tcgcggaagc
     5881 ccatgtgtac caacagatct acgcgccggt gccgatggag acacgcggca tggtggtcga
     5941 gtgggaggcg tcgtcggagg aactgacggt gtgggcgtcc acccagacgc cgcacgagct
     6001 acgggcgttc gccgcgcgac tgttgggcat cccggcgcag ggcgtgcggg tcatcatgcg
     6061 cgacaccggc ggtgggttcg gccagaaggt cgtcccgatg cgcgaagaca tgtgcatcct
     6121 gctggccgcg cgcaaggtgc cgttcgcgct gaaatggatt gaggacaggc gcgagaacct
     6181 gatgtcggca ggccaagcgc gacatgtcga cggcaaggcc agaatggcgt tcgacgacga
     6241 gggcaacatc ctcgcggccg acatcgactt catatacgac atcggcgcat acccgacgcc
     6301 ctacccggtg ctgaccaccg cggccgtcgg catgttcttc ccagggccgt atcgcgtccc
     6361 caaggccagc ttcaactaca agacggtgtt ctcgaacacc gcgggcctgg ccgcctaccg
     6421 cggcccgtgg cagtacgaaa ccctcacgcg cgaaatcctt ctcgatatcg cggcgcgcaa
     6481 gatcggcatg gaccccgtcg agctgcgccg ccgcaacatc ctgcgcggcg acgagatgcc
     6541 ctacttcaat cccaacggca tgccctacga ccacgtcgcg cctgcggaca gcctcgagca
     6601 ggccgtgaag atcctcgacc acgaaggttt ccgtcgcgaa caacgagaag cccttgaaca
     6661 gggtcgctat ctcggcctcg gcttctcggc gtacatcgaa ccgacgggcg cggcgacggg
     6721 ccacctcgcc accgaaggcg cgaccattcg gatggagccg accggcaaga tcaacgttta
     6781 cgtgaacggc gggtcgaccg gcaacagcct cgaaacgacg gttgtgcagc tgactgccga
     6841 cgcgctgggc gccaacatcg acgatgtctc cacgattcag ggcgatacgg cggtgacgcc
     6901 gtatggcgcg ggcacgcagg gcagccgcag cggtccgatg acggcgggcg ccgtcaacga
     6961 ggcgggcacg atgctgcgca agcagattgt ggcgatggcc gcggcccgac tcggcgtcga
     7021 cgaggacgcg atcgagctcg gcggctcggt ggcctccgta cgcgaagacc cgtcgaagag
     7081 cgtgactttc gccgacatcg cctaccgctc gtattacgaa cctcagcagc tgccaccggg
     7141 catgccgccg acgctggagg cgacggcgcg gttcacatcg caggcaccga tccactgggc
     7201 gaacgccacc cacgcttgca cctgcgaggt cgacgtcacg acgggccagg tcacgctgac
     7261 gcgctacatc gtcagcgagg acgtcggcgc gatgatcaac cccaatgtgg ttgagggtca
     7321 gatcgcgggc ggcaccgtcc agggtatcgg cggcgcgctg ctggagaaca tggtgtatga
     7381 cgacgacgga aatccgctcg cgtcaacgtt tgtcgactat ctgctgccga cggccaccga
     7441 tgtaccgcca atcgaatatg gccacatcga gatcccgggg cctggtgttg gcggctacaa
     7501 gggcgtcggc gagggaggcg ccatcggctc gacgcccgcg gtgatcaacg cgatcaatga
     7561 cgcgttggcg ccgctcggtg tgacgctgac gcggttgccg gcatcgccgg ctgcgatcgt
     7621 cgaattgatc gaacaagcac gaaaggacca ctaaccgtgg agctcaacaa tgaattccgg
     7681 gttgcggtcc ccgcggccaa gacctgggag ttcctcaccg acgtcgaacg tgtggcgccc
     7741 tgcctgcccg gcgccaccct gctgtccgtc gacggtgacg agttcaccgg cgcggtcaag
     7801 gtcaaggtcg gcccaatcac ggtgtcctac aagggcgaag ccgcttttca ggagaaggac
     7861 gcaaccgctc accgcatggt gctcagggcc aacggcaagg agacccgggg caacgggaac
     7921 gcagccgccg tcgtcaccgc gcagctcaag gacgagggcg acgcgaccag cgtggtgatc
     7981 accacagacc tgacgatctc cggcaaggcg gcccagttcg gccgcggcgt cctcgccgat
     8041 gtcgcaggca acctgatcgg acagttcgcc aaggcgctgg aagcggactt gctcggcggc
     8101 gcgccggcgg ctcaaaccac gagcaccgca agcgaaccca cagcgggagc cgctgctggt
     8161 gattccgtcg acctgctcaa ggtggtggcg atgccgatgg ccaagcgagc cgcgcccgcg
     8221 ctggtcgcgc tggtggcagg tgcggccatc ggcttcctgt tggggcgcag gaaccgccag
     8281 tcatgaaggc tgccgcgttc gcctatcacc gccccgacgc cgtcgaggag gccgtggatc
     8341 tgctcgccga gtttggcgat gatgcgaaga tcctcgccgg cgggcagagt ttggtgccca
     8401 tgctggcgat gcgcctcacg cacttcgaca acctcgtcga catctcccgg atcgacgagc
     8461 ttgtcggtat cgacttgtgt gacggtgaaa tccgcatcgg agcggccacc ccacacgcat
     8521 tcgtggggat ggacgacgag gtggccgatt cggtgccact gctgacgctg gcgaccccgt
     8581 acatcggcca cttccagatc cgcacccgcg gcacgctggg cggtgcgatc gcccatgccg
     8641 atccggcggc ggagtacgcc gcggtggcgc tggcgctcga tgcgcagatg gaagccgtgt
     8701 cgccgcgggg cacccgcgat atcccggcga aggagttctt caccggcctg tgggaaaact
     8761 cgatggcgac cgatgagatc ctcactgcag tgcggttccc ggtgtggggc gcgcggtctg
     8821 ggttcgcggt cgaagagttc gcccgcaggc atggggattt cgcgattgcc ggtgctgcgg
     8881 tcgccgtcga gctggatgac gattcgcgtg taagccgttg cggcataggg cttctcggcc
     8941 tggggtcgac gccgctgcgc ggttcgcctg ccgaagaggc cgttattggg cgttcggtcg
     9001 acgagatcac cgcagacgag atcggccaac tggcgatcag cgcactcgcc gagatcccgg
     9061 ccgacctgca agggtccgcc acctatcgag ccggggtcgg cgccacgatg gtggcccgag
     9121 cgtggaaatc tgcaaccgcg gaggcaagca atgcatgagt ggccagtgca ggtgtcagtc
     9181 aacggacggc ccacccaagc atccgtggaa ccacgactca cactggcgga ttaccttcgc
     9241 gagaggtgcg gcctgacggg cacgcatctg ggctgtgagc acggcgcgtg tggggcgtgc
     9301 acggtgctcg tcgatgggca ggcggtgcgc tcgtgcctga tgttcgccgt acaggcggac
     9361 gggtgtgagg tgacgacggt cgagggcatc gcatccgagg acggcgagct ctcacccgtg
     9421 cagtccgcgc tgcgcgaatg ccatgggttg caatgcgggt tctgcacgcc ggggttcgtc
     9481 gtgtcgatca ccgcgctgct gcgggacaac ccgaagccca ccgacgagga gatccgcgaa
     9541 ggcctgtcag ggaatttctg tcgctgcacc ggttaccagg gcattgtgaa tgcggttcgc
     9601 cgtgctgccg aggtgaatgc ggaggttttg ggctgaggtc ctctttgctc gcgtactgtg
     9661 ccgtatgggt cgggacgtga caggaggggc agatgagcat tcgacgcctg gtcgcagccg
     9721 tcaccgtcgc cgtgggttac tccgttggcc tggctgcgcc cgctcaggcc gatcccggaa
     9781 ccgtgagagc gttaccaagt ggtcgcagga cgccagcatc agcttcatca ccctttcggc
     9841 gcgaacatat tgcgacgaca tgaccgccaa actgcagcag gccgcacaag cgccggcacc
     9901 ggcgcagcag gcggcgcccg gccccagcgg ccagcagacc gatgagggtt tcctgaatgc
     9961 gctccagaag cgcacagtgc cgattatttc cgactcggcc gccatcgacc tcgctcactc
    10021 gacgtgcgca cttctggaac gcggcggtac ggcacaggaa gccgtgtctc acgtcgggcg
    10081 ctccgacgcc ctgcggcact acccgaacag tcaagaccgc gaactcaaga acctccaggc
    10141 gttcacatcc attgctgtcg gcgcgtactg cccgcagatg acccaatagc tcacaggcgc
    10201 acgcggcatc ctggacgtca tgaagattgg ctttatcggg ctgggaaaca tgggcgcagg
    10261 catggccgcg aacctgctga aggcggggca cgaagtgacg gcgtacaacc ggtcgcagga
    10321 caaggttgca gcgctggcga aacagggcgc gaagccggcg aagtcggtgg cggagacgtg
    10381 cggcggcgac gtcgtcgtca cgatgctggc caacgacgac gcggtcgcag cggtcgcgtt
    10441 cggggaccac ggcatcatcg cgtcgctgcg gccgggcgcg actcacgtct cgtcgagcac
    10501 gatcagcgtc gcgttatcgg agcggctgac ggcggcgcat gctgaggcgg gtcagcagtt
    10561 tgtggcggcg ccggtgttcg gcaggcccga agcagccgca gccgcaaagc ttttcgtggt
    10621 cgcggcaggc gctgtcgcgg cgctgcaacc cctttcgccg gtgttcgacg cgatcggtca
    10681 acggacgttc gtggtgtcag aggaaccgac ggcggccaat ctggtgaaac tgagcggtaa
    10741 ctttctgatc gcgtcggtga tcgaatcgct cggcgaggcc atggctttgg tggccaaggc
    10801 aggtgtcgac aagaacgact acctcgagat ccttacgtcg acgttgttcg gtgcgccgat
    10861 atacaagacg tacggtggcc tcatcgcccg ggaggaattc gagccggctg gtttcgcagc
    10921 cgaactcggg ctcaaggaca tccgcctggt actcgcagcc ggtgaaacac tgcagacgcc
    10981 attgccgatc gccagcctct tgcgggatcg cttcctgaca ctgctcgcca acggcggcgg
    11041 cagcctggac tggtcggcgg tgggcgcgct gcccgcatgg gaggccggcg gatcgagccc
    11101 gacagcacgg gattagcgag gaacgagggc caacagcggt tcgggcgtgg gaccatttgc
    11161 tggtggatcg ggaagatccg gagtttcgca ttgcggacga acgtcaagga cagcgtcgac
    11221 ggcaccgtag cggcggcccc ggcaagctcg atctggggaa tgccttctgg tgggtcgtcg
    11281 cgctgatcgg ccctgtggtt ggcgccgcgt gggtgctgac gctagccctc ccttcgagcg
    11341 cactgtggat gagccggatc gtgtgcagtg gttcgcacaa catggagcac cgcggcccgc
    11401 atttcgaatg cgtcagcgac gccggctcgc ataccgtcaa cattctcgcg gtcgtggggg
    11461 ctccaagcct tgctcatcgc gctcgtgctg tgcgccgtcg tggcagtcag cgtcgtggct
    11521 cagcggcagc tgcggaagcg cacgtaacgg tcccgctcag aacggtggtg ggtcctcttc
    11581 acccggcggg ggcggtcgtg atgggaaata ggcgtcgtcc cag
//
LOCUS       DASRJA010000296         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2131913,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000296 DASRJA010000000
VERSION     DASRJA010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2131913"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VFC01_12915"
     CDS             complement(<1..493)
                     /locus_tag="VFC01_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN80568.1"
                     /translation="MTAPTMDEAARVFADPLAYTDEAKLHAALMHLRTHAPVAWVDVP
                     SYRPFWAITKHADIMAIERDNTSFTNSPRPVLMTEEADEQQAAIGISTLIHMDDPQHR
                     VVRAIGADWFRPKAMRALKVRVDELAKRFVDNMMESDECDFVQEVAVNYPLYVIMSML
                     GLPE"
     gene            complement(490..1536)
                     /locus_tag="VFC01_12920"
     CDS             complement(490..1536)
                     /locus_tag="VFC01_12920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN80569.1"
                     /translation="MGSDVGLKVDAAVSSQLTNVAQAAAKLERRGYDCCWTAEINHDP
                     FLPLVLAAEHTTRIELGTSIAVAFARNPMTVANVGWDLQEYSDGRFILGLGSQVQAHI
                     EKRFSMPWSQPVRRMREFVLALHEIWGCWRDGTKLRFEGDFYTHKLMTPMFTPEPQPH
                     PFPKVFVAAVGEVMTEMCGEVADGLLAHAFTTKRYFEEVTIPALRRGMERSGRQRSDF
                     QLSAPIFVVTGETEGELSAGAAGTRKQIAFYGSTPAYRKVLELHGWGDLHTELHRLSL
                     QGEWDVMGSLIDDEILEAFAVVAPLDEVASKIRDRCDGVIDRVLVGFPSSVPEATVVA
                     VLDELRGQPVRSRP"
     gene            1623..>2290
                     /locus_tag="VFC01_12925"
     CDS             1623..>2290
                     /locus_tag="VFC01_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZN80570.1"
                     /translation="MTKAEDWGETLDELSRRRRHAHAMGGTERLVKHHGKGKLDARAR
                     LEHLLDKDSFREFGTLVGGDIAADGIVAGSGLINGSPVMVGAEDFTTLAGSIGPGGNA
                     KRYRLAELALRDRIPLVMLLEGAGFRPSGEHYGRTPTDLLAQAQCSGKVPMVGGLLGP
                     SAGHGALVAPVCDFTIMSRQGAIFTAGPPVVKESTGEDISKEDLGGPSVALASGVIHN
                     LAED"
BASE COUNT          394 a          747 c          759 g          390 t
ORIGIN      
        1 cttcgggaag gccaagcatc gacatgatga cgtacagcgg atagttgacc gcgacctctt
       61 ggacgaagtc gcattcatcg gattccatca tgttgtcgac gaaacgcttg gcgagttcgt
      121 cgacgcgaac cttcaacgcc cgcatggcct tcgggcggaa ccagtcggcg ccgatcgccc
      181 gcaccacgcg gtgttggggg tcgtccatgt ggatcagcgt gctgattccg attgcagcct
      241 gttgctcgtc ggcttcctct gtcatgagca ccggccgcgg cgagttggtg aacgacgtgt
      301 tgtcgcgctc gatcgccatg atgtcggcgt gcttggtgat ggcccagaac ggccggtacg
      361 acggcacgtc cacccacgcg accggggcgt gggtgcgcaa atgcatcagg gccgcatgca
      421 gtttcgcctc gtcggtgtac gccagcgggt cggcgaacac cctggctgct tcgtccatcg
      481 tcggcgcggt cacgggcgac tcctcacagg ttggccgcgc agctcgtcca gcacggcaac
      541 gacggtggcc tccggcacag aggatgggaa accgaccagc acgcggtcga tcacaccgtc
      601 acaccggtcg cggatcttgc tcgccacctc atcgagagga gcgacaaccg cgaacgcctc
      661 gaggatctcg tcgtcgatga gcgaccccat gacgtcccac tcgccctgaa ggcttagccg
      721 gtgcagttcg gtgtgcaggt ctccccagcc gtgcagctcg agcaccttgc ggtacgcggg
      781 tgtcgaaccg tagaaggcga tctgctttcg cgtgccggcg gcgcccgccg acaattcgcc
      841 ttcggtctcc cccgtcacca cgaagatcgg agcggacagc tggaagtcgc tgcgctggcg
      901 cccggaccgc tccatgcccc gccgtagcgc agggatggtc acctcttcga agtaccgctt
      961 tgtggtgaaa gcgtgcgcga gcaggccgtc ggcgacctcg ccgcacatct ccgtcatcac
     1021 ctcgccgacc gcggcgacaa acaccttcgg aaacgggtgt ggttgcggct cgggcgtgaa
     1081 catcggcgtc atcagcttgt gggtgtagaa gtcgccctcg aagcgcagct tcgtgccgtc
     1141 gcgccagcac ccccagatct cgtgcagcgc cagcacgaac tcgcgcatcc gccgcaccgg
     1201 ttgactccac ggcatgctga accgcttctc gatgtgggcc tgcacttgcg agccgagacc
     1261 gaggatgaat cggccgtccg agtactcctg cagatcccac ccgacgttgg cgactgtcat
     1321 gggattgcgg gcgaatgcca cggcgatgct ggtgcccagt tcgatccgtg tggtgtgctc
     1381 ggccgccagc accaacggca ggaagggatc gtggttgatc tcggctgtcc agcagcagtc
     1441 gtagcctcgc cgctcgagct tggccgccgc ctgcgcgacg ttcgtgagct ggctggacac
     1501 ggccgcgtcg accttcaggc cgacgtcgct acccatgccc gcagacgcta cagtaagcgc
     1561 ttcagtatgg tcaacgtgaa catgccggtg cgacgatcgc cttctgacga caggagtggc
     1621 ccatgacaaa ggccgaggac tggggagaga cgctcgacga gctgtcccgc cgacgtcgac
     1681 acgcgcatgc gatgggcggg acggaacggc tcgtcaagca ccacggcaag ggcaagctcg
     1741 atgcccgcgc acgccttgag caccttctcg acaaggactc gttccgagag ttcggaacgc
     1801 tcgtcggcgg tgacatcgcg gccgacggca tcgtcgccgg gtccggcctc atcaacggtt
     1861 caccggtgat ggtcggcgca gaggacttca cgacgctggc gggcagcatc gggccgggcg
     1921 gaaacgccaa gcgctaccgg ctggccgagc tcgcgttgcg cgacaggatt ccactggtga
     1981 tgcttctgga aggcgcaggc ttccggccca gcggtgagca ttacgggcgc acccccaccg
     2041 acctgctggc gcaggcgcag tgctccggca aggtgccgat ggtcgggggc ttgcttggcc
     2101 cctcggcagg acacggtgca ctggtggcac cggtgtgcga cttcaccatc atgagtcgac
     2161 agggcgcgat cttcaccgca ggcccgccgg tggtcaaaga gtcgacgggc gaggacattt
     2221 cgaaggaaga cctcggcggg ccgagcgtcg cgctggccag tggggtgata cacaacctgg
     2281 ccgaggatga
//
LOCUS       DASRJA010000297         4963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1824440,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000297 DASRJA010000000
VERSION     DASRJA010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824440"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFC01_12930"
     CDS             complement(<1..755)
                     /locus_tag="VFC01_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN80571.1"
                     /translation="MTTRHDVAFTSAGITCQAWLYLPPSPEPPPIVVMAHGFGGIRQM
                     RLDAYADRFTRAGYACLVFDYRHFGASGGQPRQLLNIKRQLADWAAAIRFARARDDVD
                     GSRIILWGTSFSGGYVITTAARHTGITAVIAQCPFTDGMTSVLAMPRTTALKVGGRAL
                     IDLAAAKLGKDPALMPVVGPPGSTAVMTAPDALPAMQAMVPDGVPWRNEVAARVALDV
                     LTYRPGRAAARVQCPILFCICQADTVTPADTTLR"
     gene            complement(805..1698)
                     /locus_tag="VFC01_12935"
     CDS             complement(805..1698)
                     /locus_tag="VFC01_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN80572.1"
                     /translation="MSDLHIAWTLSTPPSRQHLDVSARYRKLEALTIGEFRTTPLKGR
                     RPPQCVSETPSVGVLMNLAGHLMCRYAEGDQFILEPGHLAIWDSETAHEFEALEAHHE
                     LYVLLPRERVPAGLARAAQRPALSAPAGPGSGLLAIAAQQLRAITRELDRLSDAGLAI
                     ACQALFDVLDSALSPPVQQPSTNAALLIKVKQYIEDHLDDAQLSPSGIAAAHAISVRS
                     LHLLFADTGTTVSRWIRDRRLKICYRELSRAGHKDTVTNVAFRWGFNDSAHFSRTFKE
                     AFGVTPSSILTGTKSRYSHAR"
     gene            1969..3093
                     /locus_tag="VFC01_12940"
     CDS             1969..3093
                     /locus_tag="VFC01_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5938 domain-containing protein"
                     /protein_id="HZN80573.1"
                     /translation="MSDPRPVVIYGASGYTGRLVAEFLREYSIPFVAAGRNKAKLQEV
                     MNSVPGIETADYEIAAVDGSPASLEALFDGRKVVCNMVGPFLRYAPPVLAAALNAGCH
                     YLDTAGEQQHHLSVLDDWGPKFAAAGLAASTAASIQYALPDIAARIVLETPGIDTLEL
                     GEYVNAVPTVGSTQSIFDVIRTDAFYLEDNVLKKYDGIYEQDVVVPGTGYVVPGLNWG
                     GTAMPVFFRDDRRVRNCRMLVAMQNQARDIPQRVRAAERMFKVVFQWMPEEKLYPVLD
                     RVAAGMTPTTPPRENRQVHRSIDWCTGRGNNVTARAVIHGTAGYQMTGLIQAYAALRL
                     LGNNFNGVGFRSPAELLGHRELLGALESYGLARLTEESVS"
     gene            3090..4316
                     /locus_tag="VFC01_12945"
     CDS             3090..4316
                     /locus_tag="VFC01_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZN80574.1"
                     /translation="MTETPVWIVGVGMTTFGVRSDASIKDLTREAVNSALADAGAELR
                     DVQAAYFGNTCQDILEGQVVVPGQMALRSMGFERIPMVNVENACATGASALHQAAMHV
                     RSGAADVALAVGVEKLNVGDKRKSLAVFDGGVDVHDREGVRALLTQLGGQPPASTDTH
                     SLFMDYYAALAHAHMDEFGTTQHQLAIIAEKNHAHAVHNPLAHFRTALSAQEILAARP
                     VAGPLTVPMCSPLTDGAAAAVVCNAAGARRLRQGSRGIRVLACALGTGTDRPVHAWDR
                     SVSRWTAQQAYEQAGLGPEDIAVAEVHDASAFGELLQTEMLGFCEIGAGGKLAETGET
                     TLGGRLPVNPSGGLESKGHPMGASGLAQIYELVHQLRGDCGPRQVHGARVALAENGGG
                     LYAGEEAVAAVTILSS"
     gene            4347..>4963
                     /locus_tag="VFC01_12950"
     CDS             4347..>4963
                     /locus_tag="VFC01_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN80575.1"
                     /translation="MTYQTLNLRRDDRILYVDFNNPPLNLMNVQMVGELFDLAGSLAF
                     DPETAVVVIGSSDPEFFMAHFDLHDLTRVISGDPDVPQSRYDDINAVQALTTTWQTLR
                     QVTIGVVDGICRGVGLEFLLATDMRFASPESTFGLPETSGGILPAGGGTTRLAMQIGP
                     ARALEVLLSARDFTGEEAAAYGIVNRTVARSDLKSYIDALARSIA"
BASE COUNT          887 a         1544 c         1655 g          877 t
ORIGIN      
        1 cgcagcgtcg tgtcagcggg tgtgacggta tctgcctggc agatgcagaa caggatcgga
       61 cattgcacgc gggcggcggc gcggcctggg cgataggtca gcacgtcaag cgccacgcgc
      121 gcagccacct cgttacgcca cggcacaccg tctgggacca tcgcttgcat ggcgggcaac
      181 gcgtcagggg ccgtcatgac tgcggtggaa ccgggcgggc cgacgacggg catcagcgcg
      241 ggatccttgc cgagcttggc cgctgccagg tcgatgagtg cgcggccgcc gaccttcagc
      301 gcggttgttc ggggcatcgc cagcactgag gtcatcccgt cggtgaacgg gcactgcgcg
      361 atcacagcgg tgatgccggt gtggcgagcc gcggttgtga ttacgtaacc gccgctaaat
      421 gacgttcccc acaaaatgat tcgagaaccg tcgacatcgt cgcgggcgcg cgcgaatctg
      481 atcgcggccg cccagtctgc caattggcgc ttgatgttga gaagctgccg aggctgaccg
      541 ccgctggcgc cgaaatgtcg atagtcgaag acgaggcatg cgtagccggc tcgggtgaat
      601 cggtcggcgt aggcgtccag tcgcatttgg cggatgccgc cgaatccgtg ggccatcacg
      661 acgatcggtg gaggttcggg ggagggcggc agatacagcc atgcctggca cgtgatgccc
      721 gccgaggtga acgccacgtc gtgtcgcgtt gtcatgcgcg ctctcttcgt tgggtcggtc
      781 gccgggcggc tgcgacgctc tggcctatcg ggcatgtgaa taccgggatt tcgtaccggt
      841 gaggatgctg ctgggggtga cgccgaaggc ttctttgaat gtgcggctga agtgcgctga
      901 gtcgttgaat ccccagcgaa acgccacgtt ggtcacggtg tccttgtggc cggcgcggga
      961 aagctcgcgg taacagattt tcaaccggcg atcacggatc cagcggctga ccgttgtgcc
     1021 ggtgtcggcg aacagaagat gcagactgcg caccgagatg gcatgtgctg ctgcgattcc
     1081 tgacgggctc agctgggcgt cgtcgaggtg atcttcgatg tactgcttca ctttgatcag
     1141 gagcgcggcg ttggttgacg gttgttgtac cgggggactc agtgcgctgt cgagtacgtc
     1201 gaataacgcc tgacaggcga tcgcgagtcc ggcatcggaa agccggtcga gctcacgggt
     1261 gatggcgcgc aactgctggg cggcgatcgc cagcagaccc gacccgggcc ccgcgggtgc
     1321 ggataatgcc ggtcgctgcg cggccctggc cagcccggcg gggacacgct cgcgaggcag
     1381 cagcacgtat agctcgtggt gggcttcgag ggcttcgaat tcgtgggcgg tttcgctgtc
     1441 ccagatggct agatgaccgg gctccaggat gaattggtct ccctcggcgt atcgacacat
     1501 cagatggcct gcgagattca tgagcacgcc gaccgacggt gtctcggata cacactgcgg
     1561 tggacggcga cccttcagcg gggtggtgcg aaactcgccg atcgtcagcg cctccagttt
     1621 gcggtatcgc gccgacacgt ccaggtgctg tcgactcggg ggagtcgaca gcgtccaagc
     1681 gatgtggagg tcggacaacc caccgcctcc ggtgtcatcg tccgtcctac gggatgaaac
     1741 gtgggtagac cacgtcgccg tcgctgtcat tagcgcttgt cacctccgtt ggcgagaagc
     1801 tcagtacaga tgcgccacag gtgcgtgaca agcgcgtgtg ccgtcaggcg gaatggcccg
     1861 ctggcgccat cagtcaaaga tgtctgcgct gtcgtgcaag cggcgaccca gccaccgtcg
     1921 cgaaagtgat ggccaacaca tcacacggag acgagcaaag gagcagccgt gagcgatccc
     1981 cgcccagttg tcatctatgg agccagcgga tacaccggcc gtctagtggc cgagttcttg
     2041 cgcgagtaca gcatcccctt cgtggcggcc ggccggaaca aggccaagct gcaagaagtc
     2101 atgaactctg tacccggtat cgagaccgcc gattacgaga tcgcagccgt cgacggctcc
     2161 ccggcctcgt tggaggcact gttcgacggc cgcaaggtcg tctgcaatat ggtcggcccc
     2221 ttcctgcgct atgcgccccc ggtgctggcg gccgcgctga atgcgggttg ccactacctc
     2281 gacacagccg gcgagcagca acaccaccta tcggtgctcg acgactgggg tccgaaattc
     2341 gccgcagctg gactcgcggc atcgaccgcg gcgtccattc agtacgcgct gcccgacatc
     2401 gcggcgcgca tcgtgctcga gacacccggc atcgacaccc tggagttggg cgagtacgtc
     2461 aacgccgtcc ccaccgtcgg ctcgacccag tcgatcttcg acgtgatccg caccgacgct
     2521 ttctacctag aggacaacgt cctcaagaag tacgacggca tctacgagca ggatgtcgtg
     2581 gtaccgggga cgggttatgt tgtcccgggg ctgaattggg gcggaaccgc gatgccggtg
     2641 ttcttccgtg acgatcgtcg agttcgcaac tgccgcatgc tggtggcgat gcaaaaccag
     2701 gcccgcgaca ttccgcagcg tgtgcgtgcc gccgagcgga tgttcaaagt tgtcttccag
     2761 tggatgcccg aggagaagct gtacccagtg ctcgaccggg tagccgccgg catgaccccg
     2821 acgacgccgc ctagagagaa ccggcaggtg caccgctcca tcgactggtg caccggccgc
     2881 ggcaacaatg tgaccgcacg cgcggtcatt catggaaccg ccggctatca gatgaccggg
     2941 ctcattcagg cgtatgccgc tttacgcctg ctgggcaaca acttcaacgg cgtcgggttc
     3001 cggtcgccgg ccgaactgct gggccaccga gaactcctgg gggcgctgga gtcctacggg
     3061 ctggcgcggc tcaccgagga gtcggtgtcg tgaccgaaac acctgtgtgg atcgtcgggg
     3121 tgggcatgac aaccttcggc gtacgctccg acgcatcgat caaggacctc actcgggagg
     3181 cggtgaattc ggcgctggcc gacgctggag ccgagctgcg tgacgtgcag gccgcgtact
     3241 tcggcaacac ttgtcaggac atcctcgagg gtcaagtcgt agtgcccgga cagatggcgc
     3301 ttcgctcgat gggctttgaa cgtatcccga tggtcaacgt cgaaaatgcc tgcgccaccg
     3361 gcgcttcggc actgcatcag gcggccatgc acgtgaggtc gggagccgcg gacgtggcgt
     3421 tggcggtggg cgtagagaag ttgaacgtcg gcgacaaacg caaatcgttg gcggtgttcg
     3481 acggcggagt cgacgtccac gaccgcgagg gcgtccgggc gctgctcacg cagctgggcg
     3541 gtcaaccgcc ggccagcacc gacacccaca gtctgttcat ggactactac gccgcgttgg
     3601 cgcacgccca catggatgag ttcggcacaa ctcagcatca gctggcgatc atcgcggaga
     3661 agaatcacgc ccacgcggtg cataatccgt tggctcactt ccgtactgcc ctctccgccc
     3721 aagaaatcct ggccgcacga ccggttgcgg ggccattgac ggtaccgatg tgctcgcccc
     3781 ttaccgacgg ggcggcggcg gcagtagtgt gcaacgccgc tggggcgcgc cggctgcggc
     3841 agggcagccg cggtattcgt gtgctggcgt gtgcgctggg caccggaacg gaccggcctg
     3901 tgcatgcatg ggaccgttcg gtgagccggt ggaccgcaca gcaggcctac gagcaggccg
     3961 gtctcggacc cgaggacatc gcggtggccg aggtccacga cgcctcggcg ttcggcgagc
     4021 ttctgcagac cgaaatgctc gggttctgcg aaatcggtgc cggcggaaag cttgccgaaa
     4081 cgggagagac cacgctcggt ggccggctac cggtcaaccc ctccggcggt ctggagtcca
     4141 aggggcatcc gatgggcgca tcgggtcttg cccagatcta cgagctcgtg caccagctgc
     4201 gcggtgactg cggacctcgt caggtccacg gtgcgcgtgt tgctctcgcc gaaaacggcg
     4261 gcggcctcta cgccggtgag gaggccgttg ccgccgtcac catcctcagc tcctagccaa
     4321 ccaactttca aacagaggaa tcaaccatga cgtaccaaac tctcaacctc cgccgcgacg
     4381 atcgcatcct ttacgttgac ttcaacaatc cgccgttgaa cctgatgaat gtacagatgg
     4441 ttggcgaact attcgaccta gcagggtctt tggcttttga ccccgagacc gccgtcgtgg
     4501 tgattggcag ctctgacccc gagttcttca tggcgcactt cgacttgcat gacctcaccc
     4561 gggtaatcag cggagatccg gacgtgccgc agagtcgcta cgacgacatc aacgctgtcc
     4621 aggccttgac cacgacctgg caaaccctga ggcaagtgac gatcggtgtc gtggacggca
     4681 tatgccgcgg agtcggcttg gaattcctgc tagccaccga catgcgattc gcttctccgg
     4741 agagcacgtt cggactaccc gaaaccagcg gtggaatcct cccggccgga ggcggcacca
     4801 cccgcctggc gatgcagatc gggccggccc gcgccctgga agtactgctg tcggcccgtg
     4861 acttcaccgg cgaggaggcg gccgcgtacg gcatcgtcaa ccgaacggtg gcgcgcagcg
     4921 acctcaagag ctacatcgac gccttggccc gcagcatcgc caa
//
LOCUS       DASRJA010000298         7150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_886976,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000298 DASRJA010000000
VERSION     DASRJA010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886976"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFC01_12955"
     CDS             complement(<1..497)
                     /locus_tag="VFC01_12955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZN80576.1"
                     /translation="MADPNVVLLAAEEGGGGTSNFLIPNGTFFVVLIIFLIVLGVIGK
                     WVVPPVSKVLNERDAMVKRTVEDSRKAADQFTAADEDYRSEMAKARGEASKLRDGARV
                     EGRKVLEDMRGRASDEVSSTLQQADSALKDQALAIQTDLRSSVDTLSTTLASRVLGVE
                     INAATT"
     gene            complement(506..751)
                     /locus_tag="VFC01_12960"
     CDS             complement(506..751)
                     /locus_tag="VFC01_12960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HZN80577.1"
                     /translation="MDPTIAAGALIGGGLIMAGGAIGAGIGDGIAGNALIAGIARQPE
                     AQGRLFTPFFITVGLVEAAYFINLAFMALFVFATPVG"
     gene            complement(814..1569)
                     /gene="atpB"
                     /locus_tag="VFC01_12965"
     CDS             complement(814..1569)
                     /gene="atpB"
                     /locus_tag="VFC01_12965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZN80578.1"
                     /translation="MTETFLAEAAIEVGHHETAKWFGLTVNTDTVLATAIAAVIVIGL
                     AFFLRAKVTSTGVPGGVQLFWEAITTQLRGQIETAIGMKIAPFVLPLAVTLFVFILVA
                     NWLSVLPLQYTDSSGAIHELLKPPASDINFVLALALFVFICYHAAGVWRRGIIGHPVK
                     LLKGHVTLMAPINIIEEIAKPISLSLRLFGNIFAGGILVALIALFPPYILWLPNAIWK
                     SFDLFVGAIQAFIFALLTILYFSQSMELDEDHH"
     gene            complement(1566..2012)
                     /locus_tag="VFC01_12970"
     CDS             complement(1566..2012)
                     /locus_tag="VFC01_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HZN80579.1"
                     /translation="MTTPAQDAPLVFPSVAFRPMRLLAICVVLTGLATLAAGLLGHVM
                     VGIFFGIGLVLGLLNALLVQRSVDAITADAHPLKRKMALNSATRLLVMTVIGLTIAFI
                     FRPQGLGVVFGMALFQVVLVISTALPVMKKLRAGAADGLEGEATQQ"
     gene            complement(2250..3461)
                     /locus_tag="VFC01_12975"
     CDS             complement(2250..3461)
                     /locus_tag="VFC01_12975"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HZN80580.1"
                     /translation="MEQYGATVVYAADGLLALNDRGAGVPLRELALVGLTAAIITYFA
                     TGWVRVLARRVGAVAYPRERDVHLQPIPRMGGLAMYVGVVAAVLLASQLPALTRGFVY
                     SSGMPAVVVAGGLIMFIGLIDDKWGLDALTKFAGQITAASVLVTMGVAWSVLYIPIGG
                     VGTIVLDQVSSILLTLALTVAIVNAMNFVDGLDGLAAGLGLITASAICIFSVGLLRDH
                     GGDVLFYPPAVISVVLAGACLGFLPHNFHPAKIFMGDSGSMLIGLMLAAASTTAAGPI
                     SQTAYGARDVFALMSPFLLVVAVMFVPALDMLLAVIRRTRAGVHFSTPDKMHLHHRLL
                     QIGHSHRRVVLLVYLWVGIIALGAASTIFFDPRYTGAVMLAAIVVAIVVTLIPLLRRR
                     EDRFEEMYDEK"
     gene            complement(3469..4122)
                     /locus_tag="VFC01_12980"
     CDS             complement(3469..4122)
                     /locus_tag="VFC01_12980"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZN80581.1"
                     /translation="MTEVFDCTDPGQRETGIASAISALKGGRLVVLPTDTVYGIGADA
                     FDNDAVVALLSAKGRGRDMPVPVLVGSWHTIDGLVYTVPHTARELIQAFWPGALSLVV
                     RQAPSLQWDLGDAHGTVMLRMPLHPVAIELLREVGPMAVSSANISGRPAAVTVDDARN
                     QLGDLVEVYLDGGPSEQQAASTIVDLTGAHPQVLRQGPVTLEAIAKVLGVEPESLTE"
     gene            complement(4119..4952)
                     /gene="prmC"
                     /locus_tag="VFC01_12985"
     CDS             complement(4119..4952)
                     /gene="prmC"
                     /locus_tag="VFC01_12985"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZN80582.1"
                     /translation="MSLRQVIDAAELALAEAGVGSPRTDAELLAAYAADTDRGRLAFV
                     DTGPDFHERFDELVAQRAKRIPLQHLTGTAAFGPVTVHVGPGVFIPRPETEAVLEWAV
                     AQPLSARPVIVDLCTGSGALALALSKTWPDARVIAVDDSPDALRYARRNTAGTPVELI
                     QADVTAPGLLAELDGQVDLIIANPPYIPDGAALEPEVAEHDPPHALFGGPDGMAVIDR
                     IVELAAGLLRDGGRCAIEHDDTTSARTVEAFTRTGRFADVTARQDLTGRPRFVTARRT
                     L"
     gene            complement(4949..6022)
                     /gene="prfA"
                     /locus_tag="VFC01_12990"
     CDS             complement(4949..6022)
                     /gene="prfA"
                     /locus_tag="VFC01_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZN80583.1"
                     /translation="MTDTAPAIEALLTEHADLERQLSDPNLHADPAAARKVGRRFARI
                     SPIVATFRKLEAARGDLEAARELGADDESFAAEVPGLEATVCELETQLTDLLAPRDPH
                     DGDDIVLEVKSGEGGEESALFAADLARMYIRYAERHGWTVTVLDETFSDLGGYKDATL
                     SIAAKGTPADGVWSRLKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVEEVQI
                     DESDLRIDVYRSSGKGGQGVNTTDSAVRITHLPTGIVVTCQNERSQLQNKARALQVLA
                     ARLQALAEEQAQADASADRASQIRTVDRSERIRTYNFPENRIADHRINFKAHNLDQVL
                     DGDLDALFDALAVADKQARLQQA"
     gene            complement(6110..6346)
                     /gene="rpmE"
                     /locus_tag="VFC01_12995"
     CDS             complement(6110..6346)
                     /gene="rpmE"
                     /locus_tag="VFC01_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZN80584.1"
                     /translation="MKTGIHPDYVETTVHCGCGATFTTRSTKKSGQIHVEVCSQCHPF
                     YTGKQKILDSGGRVARFEKRYGKRKAAEKSTDDK"
     gene            6535..7140
                     /locus_tag="VFC01_13000"
     CDS             6535..7140
                     /locus_tag="VFC01_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80585.1"
                     /translation="MTSVTATKNVRDRLIDAAEVCMRAKGIRSTTVSEVAEVAGVSRG
                     WLYRHFPDKVSLLGAAIVRLNDAYWSEAHAMLEQVDGLARQMALGISNARTAYDDPGA
                     LLMKLRTDEPEEFAACAGAGVQGLIPDLADFWSRYLIAARDRGEIHPATDIAEASEWV
                     ARVVLSLATVPGDTLEPRDADAVATYVRRYVIPGLRADPAL"
BASE COUNT         1260 a         2332 c         2377 g         1181 t
ORIGIN      
        1 gttgtggccg cgttgatttc gacgccgagc acccgactgg ccaaggtcgt ggacaacgtg
       61 tcgaccgacg accgcaggtc cgtctggatt gcgagggcct ggtccttcaa cgccgagtca
      121 gcctgttgca gcgttgacga cacctcatcg gaggcccggc cccgcatgtc ctccaagacc
      181 tttcgtcctt cgactcgcgc accgtcacga agtttcgacg cctcgccgcg cgccttggcc
      241 atctcggacc tgtagtcctc gtcggcggcg gtgaactggt ctgctgcctt gcggctgtct
      301 tcgacggtcc gcttcaccat cgcgtcgcgt tcgttcagca ccttcgagac aggcgggaca
      361 acccacttgc caatcacgcc gagcacgatc aagaagatga tcagcacgac gaagaaggtg
      421 ccgttgggga tgaggaagtt gctggtgcct cccccacctt cctcagccgc cagaaggacg
      481 acgttcgggt cagccatggt gggcattagc caaccggggt ggcgaacacg aacagcgcca
      541 tgaacgccag gttgatgaag tacgcggcct cgaccagacc aaccgtgatg aagaacggtg
      601 tgaacagtcg gccctgcgcc tccggctgac gggcgatgcc ggcgatcagc gcgttacccg
      661 caataccgtc accgataccg gcgccgattg caccgccggc catgatcagt ccaccgccga
      721 tgagcgcgcc ggcagcgata gtgggatcca ttccttatcc tccttgataa ctggtaggcc
      781 gtcctaccag gatttgtgac aagttcgcgg gcattagtgg tggtcctcgt ccagttccat
      841 cgactggctg aagtacaaga tcgtcagcag cgcgaagata aacgcctgga tcgcgccgac
      901 gaacaagtcg aagctcttcc agatcgcgtt cggcagccac aggatgtagg gcgggaacag
      961 cgcgatcagc gccaccagga tgccgcccgc gaagatgttg ccgaagagtc ggagcgacaa
     1021 cgagatcggc ttcgcgatct cctcgatgat gttgatcggc gccatgagtg tcacgtggcc
     1081 cttgagcagc ttcactggat ggccgatgat gccgcggcgc cagacgcccg ccgcgtggta
     1141 gcagatgaac acaaacagcg cgagcgccag cacgaagttg atgtcggatg cgggcggctt
     1201 gagcagctcg tgtatcgcac cgctggaatc ggtgtactgc agcggcaaca ccgacagcca
     1261 gttcgcaacc aagatgaaga cgaacaacgt gacggcaagc ggcaacacga acggggcgat
     1321 cttcatgccg attgcggtct cgatctgacc gcgcagctgg gtcgtgatcg cttcccagaa
     1381 cagctgaaca ccgccgggca cgcccgttga tgtgaccttg gcgcgcaaga agaatgccag
     1441 cccaatcacg atcaccgcag cgatcgcggt cgccagcacc gtgtcggtgt tgaccgtcag
     1501 cccgaaccac ttggcggtct cgtggtgacc gacctctatg gcggcttcgg cgaggaacgt
     1561 ctcggtcatt gctgtgtggc ctccccttcg agtccgtcag cggcaccggc cctcaatttc
     1621 ttcatcaccg gcagcgcggt ggagatcacc aacaccacct ggaacagggc catgccgaac
     1681 accacgccga gcccctgcgg ccggaagatg aacgcgatcg tcagcccgat caccgtcatc
     1741 accaacaggc gggtggcgga gttgagcgcc atctttcgct tcagcggatg agcgtcggcg
     1801 gtaatggcat ccaccgaccg ctgcacgagc agtgcgttga gcaaaccgag cacgaggccg
     1861 ataccgaaga agatgccgac catcacgtgg ccgagcagtc cggcggccag agttgccagg
     1921 ccggtaagca ccacacagat tgcaagcagg cgcatcggcc tgaaagcaac ggacggaaac
     1981 accaacggcg cgtcttgggc tggcgtcgtc actgcttcac ctcaatcccg cggtcacgag
     2041 agctatcccg atgattggcg tgcgtggcgc cccgagcgta tcggagggcg aagggctcgc
     2101 tggaatcacc caaggggtag ctccctttgt cggtcgttgt tccgtgccga tcggcggtga
     2161 accgatggcc cgggggccgg caacccgcga ggttttttcg ggttctagca gcaccctaac
     2221 acatggttaa gggcagaaaa gggggtctac tacttttcgt cgtacatttc ctcgaagcgg
     2281 tcctcgcggc ggcgcagaag cgggatcaga gtcaccacga tcgcgaccac aatcgcggcg
     2341 agcatgacgg ctccggtgta gcggggatcg aagaagatcg tgctcgccgc gcccagcgcg
     2401 atgatgccga cccacagata cacaagcagc accactcgcc ggtgcgaatg accgatctgc
     2461 agcaaccggt gatgcagatg catcttgtct ggcgtcgaga agtgcacgcc cgcccgagtc
     2521 cggcgaatca ccgcgaggag catgtcgagc gccggcacga acatcaccgc gacgaccaac
     2581 aggaacggcg acatcagagc gaacacgtct cgtgcgccgt aggcggtctg cgagatcggg
     2641 cctgccgcgg tggtggacgc cgcggcgagc atcagcccaa tcagcatcga gcctgaatcg
     2701 cccatgaaga tcttggccgg gtgaaagttg tgcggcagga agccgaggca cgcgccggcc
     2761 agcacaaccg agatcaccgc gggcggatag aagaggacgt cgccgccgtg gtcacgcagc
     2821 agacccacgg agaagatgca gatggccgac gcggtgatca gcccgagccc ggcagccaac
     2881 ccgtcgaggc cgtcgacgaa gttcatcgcg ttgacgatcg cgaccgtcag cgccagcgtg
     2941 agcaggatcg acgacacctg gtcgagcacg atggtgccga cgccgccgat cgggatgtag
     3001 agcacgctcc acgcgacgcc catcgtgaca agcacgctcg cggcggtgat ctggccggcg
     3061 aacttcgtca atgcgtccag gccccacttg tcgtcgatca gcccgatgaa catgatcagt
     3121 ccacccgcga cgacgaccgc gggcatgccc gacgaataca cgaagccgcg ggtcagcgcc
     3181 ggcagttgcg acgccaacag gacggcggcc accacgccga cgtacatcgc cagaccaccc
     3241 atgcgcggta tcggctgcag gtggacatcg cgctcgcggg gataggcgac cgcgccgacc
     3301 cgcctggcaa gcaccctgac ccagccggtc gcgaagtagg tgatgatcgc ggcggtcaga
     3361 ccgacgagcg caagctcacg cagcggcacg ccagcgccgc gatcgttgag agccagcagg
     3421 ccgtctgccg cgtagaccac cgtcgcaccg tactgctcca cgctgagatc actccgtgag
     3481 cgactcgggt tccacgccga gcaccttcgc gatggcctcc agcgtgaccg gaccctgccg
     3541 cagcacctgc gggtgggcgc ccgtgaggtc gacgatcgtc gaggccgcct gctgttccga
     3601 cggtccgccg tcgaggtaca cctcgactag gtcgccgagt tgattgcggg cgtcgtcgac
     3661 ggtgaccgcg gcgggacggc cggagatgtt ggcgctcgac acggccatcg gcccgacctc
     3721 gcgcagcagt tcgatcgcga ccgggtgcag cggcatgcgc aacatcaccg tgccgtgtgc
     3781 gtcgccgagg tcccattgca gcgacggggc ctggcgcacc acgagactga gcgcgccggg
     3841 ccagaacgcc tggatgagct cgcgtgcagt gtgcggcacc gtatacacga ggccgtcgat
     3901 ggtgtgccat gacccgacca gcacggggac gggcatatcg cggccgcgcc ctttggccga
     3961 cagcagggcg acgacggcgt cgttgtcgaa ggcgtccgcg ccgatgccgt agacggtgtc
     4021 ggtgggcagc acgacgagcc gaccgccttt gagcgcactg atcgccgacg cgatacccgt
     4081 ctcccgctgc cccggatcgg tgcagtcgaa cacctctgtc atagcgtcct ccgtgccgtc
     4141 acaaaccgtg gccgcccggt caggtcctgt cgtgcggtga cgtcggcgaa acgccctgtg
     4201 cgggtgaacg cttcgaccgt gcgcgccgat gtggtgtcgt cgtgctcgat cgcgcagcgg
     4261 ccaccgtcgc gcagcagccc tgcggccagc tcgacaatgc gatcgatgac cgccatgccg
     4321 tcgggccctc cgaacagtgc gtgcggcgga tcatgttcgg ccacttcggg ttccagcgcc
     4381 gcaccgtcgg gaatgtaggg cgggttggcg atgatgaggt ccacctggcc gtcgagttct
     4441 gcgagtaggc ccggagcggt gacatcggct tgaatgagtt cgaccggagt cccggcggtg
     4501 ttgcgtcggg cgtagcgcag ggcatccggg gagtcgtcga cggcgatgac gcgggcgtcg
     4561 ggccaggtct tggacagcgc gagcgccagc gcccccgagc cggtgcacag gtcgacgatg
     4621 acgggccgcg ccgagagtgg ctgcgcgaca gcccattcca acactgcctc ggtttcgggc
     4681 cgtgggatga agaccccggg gccgacgtgg acggtgacgg gaccgaatgc cgcggtaccc
     4741 gtgaggtgtt gcagcggtat ccgctttgcg cgctgggcga ccagctcatc gaagcgttcg
     4801 tgaaagtcag gtccggtgtc gacgaaggcc agccgaccgc ggtcggtgtc cgccgcgtag
     4861 gcggccagca gttcggcgtc cgtgcgcggt gatccgactc cggcctcggc cagcgccaac
     4921 tcggcggcgt cgatcacctg acgcaagctc atgcctgttg cagcctggct tgcttgtcgg
     4981 cgacggccaa cgcgtcgaac agcgcgtcga gatcgccgtc gagcacctgg tcgaggttgt
     5041 gcgccttgaa gttgatccgg tgatcggcga tccggttctc cgggaagttg tacgtgcgga
     5101 tgcgctcact gcggtcgacg gtgcggatct ggctggcgcg atcggccgac gcgtcggcct
     5161 gcgcctgctc ctcggcgagc gcctgcagcc gcgccgcgag cacctgcagg gcgcgggcct
     5221 tgttctgcag ctgagagcgc tcgttctggc acgtcacgac gatgccggtg ggcaggtggg
     5281 taatgcgcac ggcggagtcg gtggtgttga cgccctggcc gcccttaccc gacgaccggt
     5341 agacgtcgat gcgcagatcg gactcgtcga tctggacttc ctcgacctct tcgggttcgg
     5401 ggtacacgag cacgcccgcc gccgaggtgt gcacacggcc ctgcgactcg gtgaccggga
     5461 cacgctgtac gcggtgcaca ccgccctcga atttcagccg cgaccagaca ccgtcggccg
     5521 gggttccctt cgccgcgatc gacagcgtcg cgtccttgta gccgcccagg tccgagaagg
     5581 tctcgtcgag caccgtgacg gtccagccat gacgctcggc gtagcggatg tacatgcggg
     5641 caaggtcggc ggcgaacagc gcggattcct cgccgccctc ccccgacttg acctcgagca
     5701 cgatgtcgtc accatcgtgc ggatcccgcg gggcgagaag gtcggtcagc tgagtttcga
     5761 gctcgcagac cgtggcctcg aggccgggga cctcggccgc gaacgattca tcgtcagcgc
     5821 cgagttcgcg cgcggcctcc aggtcgccgc gagcggcctc cagcttgcgg aacgtggcaa
     5881 cgatcggtga gatccgtgcg aagcgtcggc cgaccttacg ggcggcagcc gggtcggcgt
     5941 gcagattcgg gtcggagagt tgccgctcga ggtcggcatg ttcggtcagc aacgcctcaa
     6001 tcgccggcgc ggtgtccgtc atgtcgccct ccttcccgac aatcgtgcct gaaacgcaaa
     6061 ccgacgcccg gcctgcgctc gcgcgtcaga tcgggcgtcg ggaagccagc tatttgtcgt
     6121 ctgtgctttt ctcggcagcc ttgcgcttgc cgtagcgctt ctcgaagcgg gccacgcggc
     6181 cgccgctgtc gaggatcttc tgcttgccgg tatagaacgg gtggcattgc gagcagacct
     6241 cgacgtggat ctgtccgctc ttcttcgtgc tgcgcgtggt gaacgtggca ccacagccgc
     6301 agtgcaccgt cgtctcgacg tagtcagggt gaatacccgt cttcatggtt tcctcttcgt
     6361 tcgtggcccc gggtcgcccc tccgcgattc ggaggtatcg gcgtgaaccg gaaccgaggg
     6421 gtcagcggtc cattatgcca gctcaaccgc cagcagccta aacgcgtgcc aggtggcggc
     6481 tattcccgcc agtgaacaca tccgcaaatg tgtctacagt ggcacaatgc tgtggtgacg
     6541 agcgttaccg ccaccaagaa cgtccgcgat cggttgatag acgccgcgga agtctgtatg
     6601 cgcgccaagg gaattcggtc cacgacagtg tcggaggtgg ccgaggtcgc cggagtgtcg
     6661 cgagggtggt tgtaccggca tttccccgac aaggtgtcgc tgttgggcgc tgcgatcgtg
     6721 cgcctcaacg atgcgtactg gtccgaggcg cacgccatgc tcgagcaggt cgacggcctg
     6781 gctcgccaga tggccttggg catcagcaac gcccgcaccg cctacgacga ccccggcgcg
     6841 ctgctgatga agctgcgaac ggatgagccc gaggagttcg cggcatgcgc aggcgcgggg
     6901 gttcaggggc tcatccccga tctggccgac ttctggtcgc gctacctgat cgccgcgcgt
     6961 gaccgtggcg agatccaccc cgccaccgac atcgccgagg cgtcggaatg ggtggcgcgc
     7021 gtcgtgcttt cgctcgccac agttcccggc gacacgctcg aaccccgtga tgccgacgcg
     7081 gtcgcgacgt acgtgcgccg ctacgtcata cccgggctgc gggccgaccc cgcgttgtga
     7141 tttcggcgcg
//
LOCUS       DASRJA010000299        25922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1992525,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000299 DASRJA010000000
VERSION     DASRJA010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 25922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25922
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992525"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFC01_13005"
     CDS             <1..573
                     /locus_tag="VFC01_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN80586.1"
                     /translation="ELTAIAPTVFTGPPGAKWEDNLRTVGAATGRLDAANGLIDGFEK
                     DADKTGADNDATHFQASVVQLTDTTMRVYGPDTFPGSVLAAVGVDRPAAQRFIDKPYV
                     EIGITDTDMENSPDFSIADGDIVYISFASPAAKDRAPTVLTSDAWRKLSANRDNRVFA
                     VNNEVWQTGEGIVAARGMLADLKWVNAPIN"
     gene            584..859
                     /locus_tag="VFC01_13010"
     CDS             584..859
                     /locus_tag="VFC01_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZN80587.1"
                     /translation="MIHVLKSTYLQPPDVVEQTRPAHLKFLEAEIDAGRLLLAGRQED
                     GSGGMLITADMSAEDAQDIVDRDPYTRAGVAHYDRLSFNGAYRAPGL"
     gene            1003..2052
                     /locus_tag="VFC01_13015"
     CDS             1003..2052
                     /locus_tag="VFC01_13015"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZN80588.1"
                     /translation="MTTTVSAYAATSATEPLAKTTINRRDVGPNDVAFDIHFAGICHS
                     DIHTVKAEWGQPNYPVVPGHEIAGIVTAVGSEVTKYKVGDRVGVGCFVDSCRECDNCK
                     AGLEQYCTGGGMVGTYNAFLKDGEPTYGGYSGAIVVDENYVLRIPDSLPLDAAAPLLC
                     AGITLYSPLRHWNAGPDKEVAIIGLGGLGHMGVKLAKAMGAHVTVLSQSLKKMEDGLR
                     LGADEYYATSDPDTFSKQAGHFDLILNTVSANLNMGDYLNLLAVDGTLVELGIPEHPM
                     AVPAFPLAGGRRSLSGSMIGGIAETQEMLDFCAEHDVRPEIEVIAPDYINEAYERVLA
                     SDVRYRFVIDTESLR"
     gene            complement(2081..2407)
                     /locus_tag="VFC01_13020"
     CDS             complement(2081..2407)
                     /locus_tag="VFC01_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HZN80589.1"
                     /translation="MTTTSLLASILNWLRAGYPEGVPGPDRVPLLALLRATPLTEDQI
                     KEVVRNITADGSTALADGEIGEDEIAAFIKDVSHHDAGPENVQRVAAKLAAAGWPLAG
                     IDDAAI"
     gene            2601..4199
                     /locus_tag="VFC01_13025"
     CDS             2601..4199
                     /locus_tag="VFC01_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN80590.1"
                     /translation="MTTESAAVEPAAAPSAGLKKGAIGIVAVIFMAVANAAPITAMTG
                     NVPIAVGFGNGLGAPAGFLFATIALTLFALGYVAMAKHITTTGAFYGFISHGLGQIWG
                     MASGLLATFAYVIFEGSLIGGCAYFANDAVNTIVGVNIPWLVFAIGAILIIAALCHFH
                     ISLTAAVLGVTLVSEVLVLLAVAFSVIVKGGGPDGFMLNQTVLLNNAFESLPAGAFGT
                     AAAAGSMAIGLFFAFWSWVGFETTAVYGEESRNPKKIIPRATLIAVIGLGLFYTFISA
                     MVLAGNGAKTSVETSISASPLDLFFGLVEANLGSFLLDVYKILLVIGSFACALAFHNA
                     ASRYLYALGREIPSSAVRSTVGATHARHGSPHIASATQAVITLVIVLMFAGFTAVQVP
                     DANGVPVDTPSLVPYTNIYGLLALIGTAAILLVQAICSAAVISYFWARKTHPGNVITT
                     LIAPLIGGVAMLYVVWLLWDNRAFAAGYAANSLVFKAGPYIILAVFVIGLAYAVWMRY
                     AKPEAYAEIGRTVIEDSHERAETS"
     gene            4304..4471
                     /locus_tag="VFC01_13030"
     CDS             4304..4471
                     /locus_tag="VFC01_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HZN80591.1"
                     /translation="MGDEDVKQFNVYLPVSLIRRVKHHAIETESSLSALVADALRTYL
                     DTHDKQSTKES"
     gene            4472..4813
                     /locus_tag="VFC01_13035"
     CDS             4472..4813
                     /locus_tag="VFC01_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN80592.1"
                     /translation="MATEGIEAVFLETHNWGKSAKFFQGLGYEIEFATDHNSGQLRRG
                     GSPYLFIAEVPETEPTRRHIVLGVAGADAFELDGSVEVVDEWEDTHWGTRLMTVRDPD
                     GREWKLQAPAK"
     gene            4810..5658
                     /locus_tag="VFC01_13040"
     CDS             4810..5658
                     /locus_tag="VFC01_13040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN80593.1"
                     /translation="MTHATSAPDSTAVRTALWRALHVELDAAPLVLDDTIGLRLADPD
                     PDWRSRGDMHPDGTRAFRASIVARARYLDDLVAAHAAAGVGQYVILGAGLDTFAQRHP
                     RVAMRIFEVDQPGTQEWKRRRLTEIGYPPSENLQFVPVDFERGEAFPDALRANGFDAD
                     RPAVVSSTGVSMYLTKEATEATLRQVAAWSGGSVLAMTFMLPLDLVDASERQMQESVE
                     AAAAGSGTPFISHYAPDEMTDMCRAAGFSAVQHVSPDDLTARYFAGRTDGLRPPSAEQ
                     LIVATV"
     gene            5670..5948
                     /locus_tag="VFC01_13045"
     CDS             5670..5948
                     /locus_tag="VFC01_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011742436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HZN80594.1"
                     /translation="MSSPTVPGGVVHKLPADLRTALISNTTALAAWKDITPLARNEFI
                     CWVEDAKQDKTRERRIRRTQEELEEGVRRPCCWPGCKHRERNGRLAPT"
     gene            complement(6151..6942)
                     /locus_tag="VFC01_13050"
     CDS             complement(6151..6942)
                     /locus_tag="VFC01_13050"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN80595.1"
                     /translation="MDSLDMILIHGSGSTGEQLAVLRDNFQQCGWNVHVPTLRHHDAT
                     VGNQAELVAGVSLRDYADDVAQLAGNLNRPLIGGFSMGGLIAQLVASRVAHRGLFCVA
                     PAPGPGMLAPLREALVLGRHFVEATPWRKPIAPSSWEDFRRCVANEQDETAARKLHNN
                     QVFESGRVFAEVYLPHLDHGHAARVDYQAETGPVLVIGGSKDRIVSTNVCRQTAAHYP
                     HGQYVEIDGADHMLVAGRFVATVLHHLHEWIADNQLTADQHLTQP"
     gene            7020..7217
                     /locus_tag="VFC01_13055"
     CDS             7020..7217
                     /locus_tag="VFC01_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80596.1"
                     /translation="MTGVQRHDSAVMDRHVLLDDALTAAPPARRRTHAAADEAERHRA
                     HVVTPPTAGSSGRCGGLAKVQ"
     gene            7357..7980
                     /locus_tag="VFC01_13060"
     CDS             7357..7980
                     /locus_tag="VFC01_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80597.1"
                     /translation="MKKTVLGAVTLCAGAPLLAVGVGTATAWADTSTAQQTTNGDLSI
                     SVSGIKVVQTGGSTAESGFLGLAIASNGSQAGAFGPGSVAIASNGSFAGTFGLLDTAI
                     ADNNSNAGTLPFGIGNTSIARDGSTAGTVGFFNRVTATNNSSAVARGAFNNVTADGGS
                     TTDISGNANSVTLRCGGSLTDPSDSSPEIVLSAQSNRIVTRAPCETG"
     gene            complement(8224..9522)
                     /locus_tag="VFC01_13065"
     CDS             complement(8224..9522)
                     /locus_tag="VFC01_13065"
                     /inference="COORDINATES: protein motif:HMM:NF019842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HZN80598.1"
                     /translation="MTVPPTPSPATVWTVSPTWGMMATQLQGSLCQSPRTCTEIHYPP
                     FTNIPQGVSDLNDAIIDSTSDPTVPTTIVFGYSEGAEVAEQWVKQHAGDPTAPDPGDL
                     TFVLIGNPTRAYGGNLVETAGGAGNPTAEAWPQSQYQVIDIAREYEGTADYPNNPSSP
                     YYLLAVVNAWAGWWFMHDTYSTVDMNDPANAVWTVGNTTYVLVPTENLPLLEGLRGLG
                     LTALANSLNTQLKAQVDHAYNRPVPFPTTAQPAPAAITPTAASAFSAPVISDPTVAPQ
                     NTTVPVKPPPARAVTTPSIDPTLSASIISDPTVMLQNTTVPLRPAPPAQTEIAPSSTA
                     PENANGKGRVVADTATTTTPADPTNVLGAVRTITPPAAQRGLDNASAAKQSTTAPTTH
                     TTATSTTSNENKAVTGKAGGNQSRTTSEIARPTPAEAAAH"
     gene            10140..10547
                     /locus_tag="VFC01_13070"
                     /pseudo
     CDS             10140..10547
                     /locus_tag="VFC01_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(10601..11575)
                     /gene="nrdF"
                     /locus_tag="VFC01_13075"
     CDS             complement(10601..11575)
                     /gene="nrdF"
                     /locus_tag="VFC01_13075"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HZN80599.1"
                     /translation="MSEGMKLIDRVSAINWNRLQDEKDAEVWDRLTGNFWLPEKVPVS
                     NDIPSWNTLTAHEKQMTMRVFTGLTLLDTIQGTVGAVSLIPDALTPHEEAVYTNIAFM
                     ESVHARSYSNIFSTLCSTAEIDDAFRWSEENPNLQRKAEIVMQYYKGDEPLKRKVAST
                     LLESFLFYSGFYLPMYWSSRAKLTNTADMIRLIIRDEAVHGYYIGYKYQKGLALEDEA
                     RRQELKDYTYELLFELYDNEVEYTQDLYDEVGLSEDVKKFLRYNANKALMNLGYEALF
                     PRDETDVNPAILSALSPNADENHDFFSGSGSSYVIGKAVNTEDEDWDF"
     gene            complement(11628..13190)
                     /locus_tag="VFC01_13080"
     CDS             complement(11628..13190)
                     /locus_tag="VFC01_13080"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN80600.1"
                     /translation="MTVADQLTESTAKQEPVHTRALIIGTGFSGLGMAIALQRQGVDF
                     VILEKADEVGGTWRDNTYPDCACDVPSHLYSFSFEPKATWSKLFSPQPEIWDYLKGVT
                     DKYGLRRYIVFNSRAARAHWDDTEYRWHVFTDTGQEYIAQFLISGAGALHIPRLPDIE
                     GLADFQGPAFHSAQWDHDVDLAGKRVAVIGTGASAIQIVPGIIGEVAELQLYQRTPPW
                     VVPRPNEPFPDWVKTAFTYVPGLRLGVRTGIYWFLEGLGYAMTKQPGLLRAVEAVGKW
                     NIRHHVADKDLRRRLTPTYRAGCKRILYSPNYYQAVADPKTKLITDRITRITPTGIVT
                     ADGTEHAVDVIVWATGFHVIDSYTYFDIKGAEGEDLVDRWNRDGLAAHRGIAVAGMPN
                     LFFLLGPNTALGHTSVVIMIESQIRYVAKAIAAVDKYGAQALAPSAAAQQRYNDELQR
                     DLADSVWNTGGCRSWYLDEHGVNRTLWSGMTWQYWRATRSLNASEYVFLGVGHDTPTQ
                     DVAVPTVVAPQG"
     gene            complement(13307..15043)
                     /locus_tag="VFC01_13085"
     CDS             complement(13307..15043)
                     /locus_tag="VFC01_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN80601.1"
                     /translation="MTELTYAPTTIEESSLPTDQAPKAPVGGRVDAVNVSRQVGARHI
                     LQELSLSIEPGELVAIAGGSGAGKTTLLEILAGLQPPSAGKVRHDGFVLGARFGAESR
                     IGYVPQDDIIHLEMPLRRTLRYAARLRLPAGTPAAEADRVVEETMRDLDLADRAEVPV
                     RALSGGQRKRASIAVELLTRPHLFFLDEPTSGLDPSTAADVMRLLRRLSERGVTVVLT
                     THEPEGIDRCDRVVFLARDGHLAFTGSPIEARRYFGVENLAEVYDRLAGEGTPAIWAQ
                     RFANSLGKPEAQPGAGLPPIPAARSNIKRTSMIRQWWLLTRRNVDVLFRNRLTLAVLL
                     GSPVLVTVMMATLFKRGAFDPGGAADVGPAQIVFWIAFDGFFFGLTYGLLQIVGEMAV
                     FRRERLAGLSVGAYVASKVAALLPVLAGVSAVLLGVLRVLGRLPAVGWDVYAFLFATI
                     VIEATSALALGLLASAAVHNAAQAALALPMLCFPQVLFGGAIIPVDKMAIAGRLMSFG
                     LSNRHAFEALGRGLDLDRYTATLPAMSAYRDTFHGGTGASLVALGAFAVVLTLATVWV
                     LDRRSRPGAHRR"
     gene            complement(15181..16773)
                     /locus_tag="VFC01_13090"
     CDS             complement(15181..16773)
                     /locus_tag="VFC01_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007298706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZN80602.1"
                     /translation="MHPTRTLHGSKKGLRRAELLAAISLAIDLGLGQPMEHMLRSSLI
                     ATRIAERMGLDDQQRATVYYANLVGWIGCHADSHELSALFGDDIAFRADTYGVDMTGL
                     PFLRLMISHVGRGLPGWERGIRSASFLLTARNQVGKLIQSHCTSAGVLSDRMGLDRQV
                     GEALAYIFERWDGRGMPNGVRGEDIPVEIRIVRLADVVEVHLRTGGLGRALEVVRSRR
                     GTQFSPEVAEVFERDAAAIVDGLLEMDVWAEALAQAPDRGRILDGDELDELLRAMADF
                     VDLKCPCSPGYSRSVADLVTAAARHLRMPAADITWLYRAGLVHGLGRLGVSNQIWEKK
                     ASLSTAEWERVRMYPYLTGRILSRVGGLESVVSLATKHEERIDGSGFPRGLAGAELTA
                     QDRLLAAAVAYQQLLEPRPLRAALDPPAAASQLRQEASAGRLDADSVEAVLAAAGHRV
                     SRRTSWSAGLTAREVEVLRLVAQGRSNREIAAELVIAEKTARNHVERVYAKLGVNNRT
                     QASLAAIDRGLMGSRVDTAAGS"
     gene            complement(16902..17342)
                     /locus_tag="VFC01_13095"
     CDS             complement(16902..17342)
                     /locus_tag="VFC01_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZN80603.1"
                     /translation="MTTSSTHTTPFVGQTAQLSRTVGDNDIALFTEISGDRNPLHYDE
                     DAAKASRFGEIVVQGGVTSAILNAVVAEELPGPGTVFLNVNWAFKAPVRCGDTITGRV
                     EVISARTDKPITELRTTVTRDDGTVVLDGTAVCYTMDIGPRTER"
     gene            complement(17272..18552)
                     /locus_tag="VFC01_13100"
     CDS             complement(17272..18552)
                     /locus_tag="VFC01_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN80604.1"
                     /translation="MMLSRTNQQRALIALVEVFGLSVWFSATAVVPTLRSEWGIGATA
                     AVWLTASVQLGFVAGAITSTVFNLADRTTPQNLLAASALGAAACTAALAFFATGLGAA
                     VPLRFLTGMLLAGVYPVGMKLMASWSASTDRGRTFGVLLAALTVGSALPHLISGLGPL
                     PWRTVLLAAAALTATGAVLASAVVRPGPHLGARASTPNPRYALALLAERGPRLVSLGY
                     FGHMWELYALWTWLPMFVRAGQGARGDAVTASNGFIAFVAIGVAGAVGCLLGGWASDR
                     FGRPPAAVAALVTSGTCCIASPIFFAAPTLVLVVFLLVWGASVIADSGVFSTALSETT
                     DTRFVGTALTAQTAIGFLLTVVTIQFVPVVADLVGWQYAFLFLAPGPLIGAVAMSALD
                     DPQHHLLKEENHDHQLYPHDAVRRSDSAALTHCR"
     gene            complement(18566..19732)
                     /locus_tag="VFC01_13105"
     CDS             complement(18566..19732)
                     /locus_tag="VFC01_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN80605.1"
                     /translation="MDHHYDAIVVGGRCSGAATAMLLARGGMRVLVIEASRPGTDTLS
                     THALMRGGVLQLHRWGLLDRVVDAGTPAIVSTEFAYGDDIETVDIRPSGGISALYAPR
                     RTVLDALLLEAAHAAGVEVCTSARVTRLLTDGGRVRGIEGIERGTGTRFRAIAPLTIG
                     ADGRNSTIAKGVDAPFRRRGTASSAILYRYFPGLPRDRYHWAYRPGVTAGVVPTSDGL
                     ACVWVGTASAEFAQRRSASLDAAFWSLLTEAAPEVARAIGDAPPVGTLRGFPGQPGWI
                     RGSAGPGWALVGDAAYFKDPITAHGITDALRDAELLARAVLDAPRGGRAQLNTLHDYE
                     RTRDRLSVPLFDITERIASYSWDMEELRGRLRELNQAMRSEVDALLVLDEMSAA"
     gene            complement(19743..20975)
                     /locus_tag="VFC01_13110"
     CDS             complement(19743..20975)
                     /locus_tag="VFC01_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZN80606.1"
                     /translation="MLKTTTIVVGAGHCGLAMSRCLADRSIDHVVLERGEVAHSWRTQ
                     RWDSLRLLTPNWMTRLPGYAYQGDDPDGYLAAPDVVELIANYAKESAAPVRANTTVTS
                     VAQDERGYLVQTDQELWHARSVVLAPGASTVPNVPALQGSVPVGITTLTPVDYRNPSQ
                     LPDGGVLVVGASASGIQIAAEVHRSGRHVTLAVGEHVRMPRTYRGKDILWWMDAAGLL
                     DERYDEIPDLIRARNLPSMQLIGSPDKATVDLNSLSQLGVQLVGRLVGIRDGIAQFSG
                     SLPNMVALADLKLERLLDTIDGWATATGFDTDGPPRRFAATAVPTHAPLTLDLSSGAI
                     RTILWATGFRPDLSWLDVGVFDRKGHVRHDGGITASPGLYLMGMPFLRRRKSTLIDGA
                     AADAADLSGHLAHYLDAN"
     gene            complement(21005..21565)
                     /locus_tag="VFC01_13115"
     CDS             complement(21005..21565)
                     /locus_tag="VFC01_13115"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZN80607.1"
                     /translation="MTTTHTAVDNGVNVEALLGAREALADTPEIAQFRWRTTVSWVGG
                     THSRSSVDPFYGFGEEQQHKTTFTYDVDHPLAFAAQDNGITPVEYVLVALGGCLTAGI
                     ASIAQQRGIQLRSVKATIEADHDIHGILGADPHIRNGFSGVRVSYKIDADATPDQVKA
                     LVAQSQKRSAVYDILTNPTHVTVELS"
     gene            21760..22569
                     /locus_tag="VFC01_13120"
     CDS             21760..22569
                     /locus_tag="VFC01_13120"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN80608.1"
                     /translation="MSVNHFHMVTVNIPRDTAPAIDRDPWEKVLTDGHASLVRARRLF
                     RYLPSAPRCKVCNNPFGGPAGRVLAAAGFSPSRKNPNLCSRCCDALPPGGAEVDVAVL
                     FADVRGSTALGQRGAASDFASLLNRFYVTATQTLLRHDAVIDKLIGDEVMAFFVRGIS
                     GPQYRRRAVQAGMDLLKAVGYGTNAGPSLEVGVAVNAGVAYVGNVGGSVVDFTALGDP
                     VNISARMQQHAAGGELLVAAGVSDDLMTKMPRRVLNLRGHAEPIDAFVVGA"
     gene            complement(22576..23316)
                     /locus_tag="VFC01_13125"
     CDS             complement(22576..23316)
                     /locus_tag="VFC01_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80609.1"
                     /translation="MRRGSRSRSSSEPGVKVDARSERWREHRKKVRSEIVDAAFRAID
                     QLGPELSLREIAEEAGTAKPKIYRHFTDKSDLFQAVGERLRDMLWAAIFPSINLSSDP
                     AREVIRRSVEQYVRLVDEHPNVLRFLIQGRFAEQSESTMRALNEGREITLAMANMFDN
                     ELRDMELDAAALELAAYSAFGSIASATDWWLGTEPDSPRRMPPDEFVAHLTTIMVGSI
                     NGTCELLGIKIDPDLPLHEGVRRREHVA"
     gene            23468..24649
                     /locus_tag="VFC01_13130"
     CDS             23468..24649
                     /locus_tag="VFC01_13130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN80610.1"
                     /translation="MTQRYDLVIAGGGPSGSAAAWQAAQTGAKVVVLDKAEFPRDKPC
                     GDGLTARATSYLQKMGLASEVAQFHRVNRVTVFSPSQWELSFPKRPGMPDHGHTVSRT
                     ELDTLLLKHAESAGAEVRQSAEVTAPIIENGRVVGVTLKSGEKVYGDAVIAADGAYSP
                     IKRALKIDSEYNGYSAIAIRSEMHANRPDSDSLDIYLKLEFQGDQLPGYGWVFPMGGG
                     RFNIGLGYVNSYKNWQSINATQFLGEFLRTLPRDWELPPIEELKKNKSVRAWRLPMGF
                     TAWPPWRPGVLFTGDSLGAGKPASGAGISKALESGLAAGECAIAALQNGGPDDFTNYA
                     QRMEAAWGKEYRRGRYFHKLLGQPRLANAGIKLIDNAAFRDRMLKALYKKAQGPQHTY
                     K"
     gene            24703..25548
                     /locus_tag="VFC01_13135"
     CDS             24703..25548
                     /locus_tag="VFC01_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013806850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN80611.1"
                     /translation="MATQPRGVAMEVKGADGTPLTYDVSGDGEPLVLIHGSWGERQTW
                     GLVIPALAESFRVISYDRRGHGESSASPDIGTVHDDVADVVAVIDELADGRAHLVGNS
                     YGACIALRMAAAHPERLARMVCHEPPMLGVLEADDDGKPIADEERRKLGEVRQRLERG
                     DYADAAEYFVERVAFGPGAWSELPPPLQQMFVKHAPTFLGELRDGDALGADLESLRQL
                     KTPILLTHSPDSPAFFAPIVERLAILLPNARRRLLSDIGHVPHMTHPDEYAPVVLDFL
                     LEQHG"
     gene            25675..>25922
                     /locus_tag="VFC01_13140"
     CDS             25675..>25922
                     /locus_tag="VFC01_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80612.1"
                     /translation="MNIKGLLATAAMTAGLAIGLAANANAAPVSDDFWECAADHPDDI
                     GVCCVFYGGDYDERTGECWIDHEAKLEEANPGPSVPPK"
BASE COUNT         4601 a         8286 c         8499 g         4536 t
ORIGIN      
        1 gagctgaccg cgatcgcgcc gacggtgttc accggtccgc ccggggcgaa atgggaggac
       61 aacttgcgca cggtcggcgc ggcgaccggt cggctggacg ccgccaacgg cctgatcgac
      121 gggttcgaaa aggacgccga caagaccggc gccgacaacg acgccaccca cttccaggcg
      181 tcggtggtac agctgaccga cacgaccatg agggtgtacg ggccagacac cttccccggc
      241 agcgtgttgg ccgctgtggg cgtggatcgc cctgcggcac aacggtttat cgacaagccc
      301 tatgtcgaga tcggaatcac cgacaccgac atggagaact cccccgactt ctccatcgcc
      361 gacggcgaca tcgtctacat ctcgttcgct tcgcccgcgg caaaggatcg cgcgccgacg
      421 gtgcttacca gcgatgcctg gcgaaagttg tcggccaacc gcgacaaccg ggtgttcgcc
      481 gtcaacaacg aggtatggca gaccggcgag ggcatcgtcg ccgcgcgggg catgctggcc
      541 gacctgaagt gggtcaacgc cccgatcaac taacgtcatc accgtgatcc acgtcttgaa
      601 gtcgacgtac ctgcaaccgc ccgacgtcgt cgagcagacc cggcccgctc acctgaaatt
      661 tctggaggcc gagatcgacg cgggccgctt gctgctcgcc ggccgtcagg aagacgggtc
      721 cggcggcatg ctgatcaccg ccgacatgag cgcggaggac gcacaggaca tcgtcgaccg
      781 agacccgtac acgcgcgccg gcgtggctca ctacgaccgg ctctcgttca acggggcata
      841 ccgcgcccca ggcctttagg ctgcaattca cagctccaac cgggaatgac ggcaggcacc
      901 tgtgccgtta gtcccgagtg agccgcaccg tagcggcaaa tccaatcagc ttcttcgctg
      961 accttggtgg cgcatgccat aaagacgaca aagaggatct atatgacgac gaccgtttcc
     1021 gcctacgctg ccacttcggc taccgagccg ctggccaaga ccacgatcaa ccgccgcgac
     1081 gtaggcccaa acgacgtcgc gttcgacatt cacttcgcgg gcatctgtca ctccgacatc
     1141 cataccgtca aggctgaatg gggccagccg aactatccgg tggtgcccgg ccatgaaatc
     1201 gccggcatcg tcaccgcggt cggctccgag gtgaccaagt acaaggtcgg cgaccgcgtc
     1261 ggtgtcggct gcttcgtcga ctcctgtcgc gaatgcgaca actgcaaagc cggtcttgag
     1321 cagtactgca ccggcggcgg catggtcggc acctacaacg ccttcctcaa ggacggcgag
     1381 ccaacctacg gcggctacag cggcgcgatc gtcgtcgacg agaactacgt actgcgcatc
     1441 cccgacagcc tcccgctgga cgccgccgca ccgctgctgt gcgcgggcat cacgctgtat
     1501 tcgccgctgc ggcactggaa tgcgggtccc gacaaggagg ttgccatcat cggcctcggg
     1561 ggcctcggcc acatgggcgt gaagctcgcc aaggcgatgg gcgcccacgt gacggtgctg
     1621 agccagtcgc tgaagaagat ggaggacggt ctgcgcctgg gcgccgacga gtactacgcg
     1681 accagcgacc ctgacacctt ctccaagcag gccggccact tcgacctcat cctgaacacg
     1741 gtgtcggcca acttgaatat gggcgattac ctgaacctgc tcgcagtgga cggcacgctg
     1801 gtcgaactgg gcatccccga gcatccgatg gccgttcctg ctttcccgct cgccggtggc
     1861 cgccgcagcc tgtccggatc gatgatcggc ggtatcgccg agacccagga gatgctggac
     1921 ttctgcgccg agcacgacgt gcgccccgaa atcgaggtca tcgcaccgga ttacatcaac
     1981 gaggcgtacg agcgcgtgct cgcaagcgac gtgcgctatc gcttcgtcat cgacacggag
     2041 tcactgcggt gacgacatcg acagcagcag cgctgtcctt ttaaatcgcg gcgtcgtcaa
     2101 tgccggccag cggccacccg gccgcggcca gcttggcggc gacccgctgg acgttctccg
     2161 gaccggcgtc gtgatgcgag acgtccttga tgaacgcggc gatctcgtcc tcaccaatct
     2221 cgccatcagc cagggccgtc gagccgtcgg cggtgatgtt gcgcaccacc tctttgatct
     2281 ggtcttccgt caaaggagtg gcgcgcagca gcgccagcag cggcacccga tcggggccgg
     2341 gcacaccttc gggatagccg gcgcgtagcc agttcagaat cgaggccaga agcgaggtgg
     2401 tggtcacgac gctcagtgtc ctggctgctt cgaacggaag ccaccatcgg gcgacacagt
     2461 tcctcggtca ttcaccgctc gtttgattgt taacgccgtg tgtcctccgg ttgtcgagtc
     2521 gacctaagag tgcgaggctc tctgcggact gcggcatcgc aggcccttta cgcactccga
     2581 tccgaaccag gaggcccgcg gtgaccactg aatccgcagc cgtcgaaccc gccgccgcac
     2641 cgtcggccgg cctgaagaag ggcgccatcg gcatcgtcgc ggtcatcttc atggccgtgg
     2701 ccaacgccgc accgatcacc gcgatgaccg gcaacgtgcc gatcgcggtg ggcttcggca
     2761 acggactggg cgcaccggca ggcttcctgt tcgcgaccat cgcgctgacg ctctttgcgc
     2821 tcggctatgt cgcgatggcg aagcacatca ccaccactgg cgcgttctat ggcttcatct
     2881 cgcacggtct cggccagatc tggggtatgg cgagcggcct gctggcgacg ttcgcctatg
     2941 tgatcttcga gggttcattg atcggcggct gcgcgtactt cgcgaatgac gcggtgaaca
     3001 ccattgtggg agtgaacatc ccgtggctgg tgttcgcgat cggtgcgatc ctgatcatcg
     3061 cggcgttgtg ccacttccac atcagcctga ctgcggcggt gctcggcgtc accctggtgt
     3121 ccgaggtgct cgtgctgttg gccgttgcgt tttcggtgat cgtcaagggc ggcgggccgg
     3181 acgggttcat gctgaaccaa accgtgttgc tgaacaacgc attcgagagc ctgcccgcgg
     3241 gcgcgttcgg cacggcggcc gcggccgggt cgatggcgat cggattgttc ttcgcgttct
     3301 ggtcgtgggt cggcttcgag acgacggccg tttacggcga ggagtcgcgc aatccgaaga
     3361 agatcatccc ccgggcgacg ctgatcgcgg tgatcgggct gggcctgttc tacacattca
     3421 tctcggcgat ggtgctggcg ggcaacggcg caaagacctc ggtcgagacg tcgatcagcg
     3481 cctcgccgct tgacctgttc ttcgggctgg tggaagccaa cctcggctca ttcctgctcg
     3541 acgtgtacaa gatcctgctg gtcatcggat cgttcgcgtg cgcgctggcg ttccacaacg
     3601 cggcgtcgcg ctacctctac gcgctgggcc gcgaaatccc gtctagcgcc gtgcgttcca
     3661 ccgtcggcgc gactcacgcc aggcatggtt cgccccacat cgcttcggcg acacaggctg
     3721 tgatcacgtt ggtgattgtg ctgatgttcg cgggattcac cgcggtgcag gtgcccgacg
     3781 ccaacggtgt tccggtggac accccctcgc tggtgcccta cacgaacatc tatgggctgc
     3841 tggcgctgat cggcacggcg gcaatcctgc tcgtgcaggc catctgctcg gcggcggtga
     3901 tctcgtactt ctgggcgcga aagacccacc cgggcaacgt gatcaccacg ctgatcgctc
     3961 cgctgatcgg cggtgtggcg atgttgtacg tggtgtggct gctgtgggac aaccgggcat
     4021 tcgccgcggg ctatgcggcc aactcgctgg tgttcaaggc cggcccgtac atcattctgg
     4081 cggtcttcgt catcggcctg gcgtacgccg tgtggatgcg gtacgccaaa ccggaggcct
     4141 acgccgagat cggccgcacg gtcatcgagg actcgcacga gcgggcggag acctcatagc
     4201 gcgaagacgc agagggcccc gacacgccgc gtgttcgggg ccttttgcgt cttctcaccg
     4261 ccgcgccgtg tcgctagatt actagcatgc tagcctggtc cacgtgggcg acgaggacgt
     4321 caagcagttc aacgtctacc tgccggtcag tttgatcagg cgggtcaaac accatgcgat
     4381 cgagacggaa tcctccctct cggcgctggt ggctgatgcg ctgcggacgt acctcgacac
     4441 ccacgacaag caatcgacga aggagagctg aatggcgacc gagggcatcg aggctgtatt
     4501 cctggagacc cacaactggg gcaagtcggc gaagttcttt cagggcctcg gttatgagat
     4561 cgagttcgcg acggaccaca actcgggcca actgcgccgc ggtggcagcc cctacctgtt
     4621 catcgccgag gttcccgaga ccgagccaac ccgacggcat atcgtgctgg gcgtggccgg
     4681 tgccgacgcg ttcgaactcg atggctccgt cgaagtcgtc gacgaatggg aggacaccca
     4741 ttggggcaca aggctgatga cggttcgcga ccccgacggc cgcgagtgga agctgcaggc
     4801 gccggccaaa tgacccacgc aacatcagca cccgacagca ccgcggtccg caccgccctg
     4861 tggcgagccc tgcacgtcga actcgacgct gcgccactcg tgctcgacga caccatcggc
     4921 ctgcggcttg ccgatcccga tccggactgg cggtcgcgcg gcgacatgca ccccgacggc
     4981 acgcgcgctt ttcgagcgtc gattgtggcg cgcgctcgat atcttgacga cctcgtcgcc
     5041 gcgcacgcgg cggccggcgt cggccagtac gtcattcttg gcgccggcct ggacaccttc
     5101 gcgcaacgcc acccacgcgt ggcgatgcgg atcttcgagg tggaccagcc cggtacgcag
     5161 gaatggaaac ggcgcaggct cacggagatt ggctatccgc ccagcgagaa cctgcagttc
     5221 gtcccggtcg acttcgagcg cggcgaggcg tttccggatg cgttgagggc caacggcttc
     5281 gatgcggatc gtccagcggt ggtctcgtcg accggcgtca gcatgtatct gacgaaggag
     5341 gcgacggaag cgacgcttcg tcaggtcgcg gcgtggtccg gcgggtcggt actggcgatg
     5401 acgttcatgc tgccgctaga cctggtcgac gcctccgaac gccagatgca ggaatccgtc
     5461 gaagcggccg cggcagggtc gggcacaccc ttcatcagtc actacgcacc cgacgagatg
     5521 actgacatgt gccgcgctgc aggcttttcc gcggtgcagc acgtcagtcc ggacgacctg
     5581 accgcacgct acttcgccgg gcgcaccgac ggcctgcgtc cgccgagcgc cgaacaactg
     5641 atcgtcgcca cggtctagac tctggccaca tgagcagccc gacagtgcca ggtggggtgg
     5701 tgcacaagtt gcctgcagac ctgcgcacgg cgctgatcag caacaccaca gcactagccg
     5761 cgtggaaaga catcacgccg ttggcgcgca acgagttcat ctgctgggtc gaggacgcca
     5821 agcaggacaa gacgcgagag cgccgcatcc gccgcacgca ggaggagctg gaggaaggcg
     5881 tgcgccgccc gtgctgctgg cccggctgca agcaccgcga gcgcaacggc cggttagcgc
     5941 cgacctagag cttgccgcga gattgcattg agggtcgtgc atcgactcga atcacaaatc
     6001 cgcgggtagg acaccctttt gtaccgtcag ctcagaagca gcagcaccgc cgctgagagc
     6061 gccatgttaa aggtgccgta ggcacctttc tagacgcaac tgtcgcatgg aagagaggtt
     6121 cactgcgctt ctagttgatg actggcgaaa ctagggttgg gttaggtgct ggtcggcggt
     6181 cagctggtta tcagctatcc attcgtggag gtggtgcagc acggtagcaa cgaatcgccc
     6241 cgcaacaagc atgtggtcgg cgccgtcgat ctctacgtac tggccgtgag ggtagtgtgc
     6301 ggcggtttgc cggcaaacgt tggtggatac gatgcggtcc ttacttccgc cgatcacaag
     6361 cactggtccg gtctccgcct ggtagtcgac gcgtgcagcg tgaccatggt cgaggtgggg
     6421 cagatacacc tctgcgaata cgcgccccga ttcgaatacc tggttgttgt gcagcttccg
     6481 ggctgctgtt tcgtcctgct cgttggcgac acatcggcga aagtcctccc agctactcgg
     6541 cgcgatgggc ttgcgccatg gggtggcctc gacgaaatga cgccccaaga cgagggcctc
     6601 ccgcaatggg gccaacatgc ccggaccggg ggcgggcgcc acacagaaaa ggccccggtg
     6661 cgccactcgt gacgccacga gttgggcgat cagcccaccc attgaaaatc ctccgatcaa
     6721 tggtcggttg aggttacctg caagctgggc aacatcatcg gcgtagtcac gcaggctgac
     6781 cccggccacc aactcggcct ggttcccgac ggttgcgtcg tggtggcgca gcgtcgggac
     6841 atgaacgttc cagccgcact gctggaagtt gtcacgtagg acagccaact gttccccggt
     6901 tgacccgctg ccgtgaatga gaatcatgtc taagctgtcc acccggatcg cctcccctgg
     6961 cacttcttcc tcatggattc tgacagccga gccccatcga attttccgaa atggcttgtg
     7021 tgaccggcgt ccagcgccac gacagcgccg tgatggaccg ccatgtcctg ctcgacgacg
     7081 ccctaaccgc agccccgcca gctcgccgcc gcactcatgc cgcggccgac gaggccgagc
     7141 gacatcgcgc ccacgtggtc acgccgccca ccgccggttc cagtggtcgt tgtggtggcc
     7201 tggccaaagt tcagtagtcg actactgatt gcgactcacc ggtagcccgc gaggatcgtt
     7261 gctgcgggac agaaaaggag gctcactcat tcatgccgag acttcaatcg ccccgaagga
     7321 cgccatgcat ggcgtgacag tgaaaggaaa gctccgatga agaaaacagt gttgggagcc
     7381 gtaacgctat gcgctggcgc tcccctcctg gcggtcggcg tcgggacagc aaccgcatgg
     7441 gcggacacga gcaccgccca gcaaaccacc aatggtgacc tgtcgatctc ggtgagcgga
     7501 atcaaggtgg tgcagaccgg cggcagtacc gcagaatcag ggttcctcgg cctggccatt
     7561 gcgtccaacg gcagccaggc cggcgcgttc gggcccggaa gtgtagccat cgccagcaac
     7621 ggcagcttcg ctgggacctt cggattactg gacacggcga tcgccgacaa caacagcaac
     7681 gcaggcaccc ttccgttcgg tattggaaat accagcatcg cgcgagacgg cagcaccgct
     7741 ggcactgtcg ggttcttcaa ccgagtgact gcaaccaata acagttccgc cgtggcgcga
     7801 ggagcgttca acaacgtcac cgcagacggc ggcagtacca ctgacataag cggcaacgcc
     7861 aacagcgtca cgctgcgctg cggaggttcg ctgactgacc cgagcgattc atcgccggag
     7921 attgttttaa gtgcccagag caaccgaatc gtcacgaggg cgccctgcga aacaggatga
     7981 gcgggtacaa acccatattc gctgcacgcg atgtgttccc ttttaggttt gtgatcatgc
     8041 tgagcggcct cgtacagacc acagataggt gctggatgac caggccgccg agccgatgcc
     8101 cgaacggaca cgagctagga cgcaatcatc tcctcgtcgg ccatgtcgca tgcctgggac
     8161 acggcggcgg aggacacacc agtgcggtcg cggtcccacc gtaaccagcc gatggcctcg
     8221 gtgttaatgt gccgcggcct cagcgggcgt cggacgggcg atttccgacg tggttctact
     8281 ttgattccca cccgccttac cggttacagc cttgttctcg ttacttgtgg tggacgtcgc
     8341 cgtggtgtga gtggtcggcg cggtggtgga ttgcttggct gcgctggcat tgtcgaggcc
     8401 ccgctgcgcg gctggcggag tgatcgtcct caccgcgccc aacacgttgg tgggatcagc
     8461 aggtgttgtc gtggttgcgg tgtcggcgac gacccggccc ttgccgttgg cgttctccgg
     8521 agcggtggac gatggcgcaa tctcggtttg tgcgggcggc gctggcctta gaggcaccgt
     8581 ggtgttctgg agcatcaccg tcgggtcgga gataatcgaa gcagacaacg tgggatcgat
     8641 ggaaggtgtg gtcaccgccc gcgccggggg cggtttcaca ggcacagtgg tgttctgggg
     8701 cgccaccgtc gggtcggaga tgaccggagc agaaaatgcg gaagcggcgg taggagtgat
     8761 cgctgcaggt gctggctggg cggttgtggg gaagggcacg gggcggttgt aggcgtggtc
     8821 cacctgggct tttagctgcg tgttgagtga attggccagc gcggttagcc ccagcccccg
     8881 cagcccctcc agcagcggga ggttctccgt cggcacaaga acgtaagtgg tgttgcccac
     8941 cgtccaaaca gcgttggcgg gatcattcat gtcgacggtc gagtaggtgt cgtgcatgaa
     9001 ccaccatccg gcccaagcat taacgacggc cagcagatag tagggacttg acgggttgtt
     9061 ggggtagtcg gcggtacctt cgtactcacg ggcgatgtcg atcacctggt attggctttg
     9121 cggccacgcc tcggcagtgg gattgccagc ccccccggcg gtctctacca ggttgccgcc
     9181 ataggcccgg gtcggatttc cgatcagcac aaaggtcagg tcgcccgggt cgggcgcggt
     9241 cggatcgcca gcgtgttgtt tcacccactg ctcggccacc tcagcgcctt cgctgtagcc
     9301 aaacacgatc gtcgtgggca ccgtcgggtc cgatgttgaa tcgatgatgg cgtcgttaag
     9361 gtcgcttacg ccctgcggga tattggtgaa cggcgggtag tggatctcgg tgcaggtcct
     9421 cggactctgg catagggatc cttgaagctg cgttgccatc atcccccatg tcgggctgac
     9481 cgtccaaacc gtggctggac tgggcgtcgg cggcaccgtc actgccgcta cggtcggtac
     9541 tgctatatgg ggcagcaaag ccgcgatcac gggtatatgc gggtctgggt cgccatacgc
     9601 cggtgcggcg agcgtcaagc aagtagctgc agccgcgatg actgcggctc gcgtttccct
     9661 catcattgtg cccccttact ggtggcacta cctaccagcc aggcgggggt agaagtgctc
     9721 caggcggaaa atgatagctg gcaaacccag gcattgtggt tgattccgca aacaagtacc
     9781 acgttggcca ccgctgaaca ctcactgcac atgcctcgag gggccagcga gtgtgcgtat
     9841 ctccgctaga cgaggctgac cggagtcggc ttcaagtgaa gttctccaat cggcccgcca
     9901 cccgctctcc tggtggcggg ccgattgctg cgggatggtt gactagcgcc ccaagctttc
     9961 acgacgccac tggtgagcgc cgttacgggg atcgcgccca gcgaggcgca gaacggattg
    10021 tcgacggcgt cgtctcatct gccgtgacaa ctggaccgac gtcctcacgc acttagccaa
    10081 ataacaaagg ctccctcatc cattccgggt gagggagcgg cactccgaag gcgcacaagc
    10141 agcgcaccgt gccaatgccc aaattcctgc gcgcttgcct ggcgcgtcaa ggcgagggcc
    10201 ggggccgcga cgacctactc ttcggcgacg gcggcgggca cctgaagcgc tcgcactcga
    10261 cgtcgggatg gttcataaag gccgtcgctg aatccggcgt tccgcacgtc acgccgcacg
    10321 acctccggca cacctcagcc agcctcgcgg tgtcagccgg ggcgaacgtc aaagcagttc
    10381 aaaaggtgct cggtcacgca tcggcggcga tgacgttgga cgtctacgcc gacctcttcg
    10441 acgacgatct ggaagctgtc gcggcctcct tgcacaatgc tcgccctcgg gaaagtgtgg
    10501 gcaaaatgtg ggcacggggg gtgagcctac tgaccgctaa ccgctgaccg gtaactcgtc
    10561 cggcgagtcg agcctccaat aagggcattg acctgcgagt ctagaagtcc cagtcttcgt
    10621 cttcggtgtt gacggcctta ccgatcacgt acgacgaacc cgaacccgag aagaagtcgt
    10681 ggttctcgtc ggcattcggt gacagtgccg acaggatcgc cgggttcaca tcggtctcgt
    10741 cgcgcgggaa caacgcctcg tagccgaggt tcatcagcgc cttgttcgcg ttgtagcgca
    10801 agaacttctt gacgtcctcg ctcagcccga cttcgtcgta aaggtcttgg gtgtattcga
    10861 cctcgttgtc gtagagctcg aacagcagct cgtaggtgta gtccttgagc tcttgcctcc
    10921 tggcctcatc ctcaagcgcc agccccttct ggtacttgta gccgatgtag tagccgtgca
    10981 cggcctcgtc gcgaatgatc agccggatca tgtcggcggt gttggtcagc ttggctcggc
    11041 tggaccagta catcggtagg tagaagcccg agtagaacag gaagctctcc agcagcgtcg
    11101 aggccacctt gcgcttcagc ggctcgtcgc ccttgtagta ctgcatgacg atctcggcct
    11161 tgcgctgaag gttcgggttc tcctccgacc agcggaacgc atcgtcgatc tccgcggtgg
    11221 agcacagcgt agagaagatg ttgctgtagc tgcgcgcgtg caccgactcc atgaacgcga
    11281 tgttggtgta gacggcttcc tcgtgcgggg tcagcgcatc ggggatcagg ctgaccgcgc
    11341 cgacagtgcc ctggatggtg tccagcagcg tcaggccggt gaagacgcgc atggtcatct
    11401 gcttctcgtg ggcggtcagc gtgttccagg acgggatgtc gttggatacc ggcaccttct
    11461 ctggcaacca gaaattaccg gtcaaccggt cccacacctc ggcgtctttt tcgtcctgca
    11521 ggcggttcca gttgatcgcc gacacacggt caatcagctt cattccctcg gacaccagaa
    11581 ccccatttcg ccgcagaagt aggtttgccg aatcggctac cccgacacta cccctggggt
    11641 gcgacaacgg tcggaaccgc gacatcttgt gttggcgtgt cgtgcccgac gccaaggaat
    11701 acgtactccg aagcgttcag cgaccgggtc gcacgccagt actgccacgt cataccgctc
    11761 cacagggttc ggttgactcc gtgctcgtcg agataccagc tgcggcaacc gccggtgttc
    11821 cataccgagt ccgccaggtc gcgctgcagt tcgtcgttgt accgctgctg ggcggcagcg
    11881 cttggcgcca gcgcctgcgc cccgtacttg tcgaccgcgg cgatcgcctt tgccacgtag
    11941 cggatctgcg attcgatcat gatgaccacc gacgtgtgcc ccagtgcggt gttgggcccg
    12001 agcagaaaga acaggttcgg catgcctgcg accgcgatgc cacggtgtgc cgccagcccg
    12061 tcgcgattcc agcgatcgac gaggtcttca ccctctgcgc ccttgatgtc gaagtaggtg
    12121 taggagtcga tgacgtggaa cccggtggcc caaacgatca cgtcgacggc atgttcggtg
    12181 ccgtcggcag tgacgatgcc ggtcggggtg atacgcgtga tccggtcggt gatcagcttc
    12241 gtcttcgggt cggcgacggc ctgatagtag ttgggcgagt acaggattcg cttgcagccg
    12301 gcgcgatatg tcggcgtcag gcggcggcgc aggtccttgt cggcgacgtg gtggcggata
    12361 ttccacttgc ccaccgcttc gaccgcacgc agaaggccag gctgtttcgt catcgcgtac
    12421 ccgagtccct cgaggaacca gtagatgccg gtccgcaccc ccagacgcaa ccccggaacg
    12481 taggtgaatg cagtcttcac ccagtccggg aacggctcgt tcggccgggg cacgacccag
    12541 ggcggcgtcc gttggtagag ctgaagttcc gcgacttcgc cgatgatgcc cggcacgatc
    12601 tggatggcgc tcgcgccggt gccgatcacc gccacacgct tgccggcgag gtcgacatcg
    12661 tggtcccact gcgcggaatg gaaagcgggg ccttggaaat cggcgagccc ttcgatgtca
    12721 ggaaggcgcg gaatgtgcag cgcgccggca ccggagatga ggaactgcgc gatgtactcc
    12781 tgcccggtgt cggtgaacac gtgccatcgg tattcggtgt catcccagtg cgcgcgggcg
    12841 gcgcgcgagt tgaacacgat gtagcgccgc aggccgtact tgtcggtgac gcccttgagg
    12901 tagtcccaga tctccggttg gggcgagaac agcttgctcc acgtcgcctt cggctcgaag
    12961 gagaacgaat agaggtggga cggcacgtcg caggcacagt ccggataggt gttatcccgc
    13021 caggtgccgc cgacttcgtc ggccttctcc aagatgacga agtcgacgcc ctgccgctgc
    13081 agtgcgatgg ccatccccag accggagaag cccgtgccga taatcagcgc gcgggtatgg
    13141 accggttcct gcttggcggt gctttcggtc agctgatcgg caacggtcac ggggaccaaa
    13201 gtaccccgta cccgcggtat cgcctagatc cgaaagtcct ccgccggcgc acggcaaggc
    13261 ccgcggaatc tcgttaaaca tcccgcgggc ccaccgggct aggccgtcat cgccgatgtg
    13321 cgccaggccg agacctccgg tccagcaccc acaccgtggc cagcgtgagg acgacggcaa
    13381 acgcgccgag cgcaacgagg ctggcgccgg tgcctccgtg gaaggtgtcg cggtaggcgg
    13441 acatggccgg caacgtggcg gtgtaccggt cgagatccag gccacgcccc agtgcctcga
    13501 atgcgtgacg gttggatagg ccgaagctca tcaggcgtcc cgcaatggcc atcttgtcaa
    13561 cggggatgat ggcgccaccg aacagaacct gcgggaagca aagcatcggc agagcgaggg
    13621 cggcctgcgc ggcgttgtgc acggcggccg aggcgagcag gccgagggcc agtgccgagg
    13681 tcgcctcgat cacaatcgtt gcgaacagga aggcgtacac gtcccacccg acggcgggga
    13741 gccgacctag cacccgcagc acaccaagaa gcaccgcgct cactccggca agcaccggaa
    13801 gcagcgctgc gaccttggat gccacgtagg cgcccacact gagcccggcc aggcgctccc
    13861 gccggaagac ggccatctcc ccgacgatct ggagcaggcc gtatgtgagg ccgaagaaga
    13921 acccgtcgaa tgcgatccag aacacgatct gcgccgggcc aacgtcggct gcgccacccg
    13981 ggtcgaacgc gccgcgcttg aacagcgtcg ccatcatcac ggtgacgagc accggtgaac
    14041 cgagcaagac cgccagggtc agccgattgc gaaacagcac atcaacattg cgacgtgtga
    14101 gtagccacca ctgtcggatc atgctggtgc gcttgatgtt tgaacgggca gccgggatgg
    14161 gcggcaaacc cgcaccaggt tgagcttccg gtttcccgag gctgttcgcg aatcgctgcg
    14221 cccagatcgc aggggtgccc tcgccagcca gccggtcgta cacctcggcg agattttcca
    14281 ccccgaagta ccgccgagcc tcgatggggc tgccggtgaa ggcgaggtgg ccgtcgcgag
    14341 ccaggaatac cacccggtca caccggtcga tgccttccgg ttcatgggtc gtgaggacga
    14401 ctgtgactcc gcgctcgctg agccgacgca acaggcgcat cacgtcggcc gcggtcgacg
    14461 gatcgagccc ggacgtcggt tcgtcgagga agaacaggtg cggccgggtc agtaactcca
    14521 cggcgatact ggcccgcttc cgctggccgc cggacagcgc acggaccggc acctcggccc
    14581 ggtcagctag gtcgaggtca cgcatggtct cttcgaccac cctgtccgcc tcagccgccg
    14641 gtgtgcccgc gggcagccgt agccgcgccg cgtagcgcag cgtgcggcgc aggggcatct
    14701 cgaggtggat gatgtcgtcc tgaggcacgt agccgatgcg tgattcggcg ccgaatcggg
    14761 caccaagaac gaagccgtcg tgccgaactt tcccggcaga cggcggttgc agtcccgcga
    14821 gaatctcgag caatgtggtc tttcctgctc cactgccgcc ggcgatggca accagctcgc
    14881 ctggttcgat tgacagcgac agctcctgca ggatgtgccg cgcaccgacc tggcggctca
    14941 cgttgactgc atcgacccgc ccgccgaccg gagccttagg tgcctgatcg gtggggagcg
    15001 agctctcctc gattgtcgtt ggtgcgtagg ttaattcggt catgacggac aacctccctg
    15061 gtctgtgaac tggcttcgtc atgacctgat catcggtccg ccgccggcgt tgcacatgag
    15121 gcgtttgcct caatctcagc ttccgctacg gcgggaggat ctcagagtcg ttgcggctct
    15181 ttatgatccc gcggcggtgt cgacccggct gcccatcagg ccgcgatcga ttgccgcgag
    15241 actcgcttgg gtacggttgt tgacacccaa ttttgcatag acgcgctcga cgtggttgcg
    15301 cgcggtcttc tcggcgatga ccagctcggc ggcaatttca cggttcgacc ggccctgcgc
    15361 gacaagtcgc aagacctcga cctcccgggc ggtaagcccc gccgaccaag atgtccgccg
    15421 cgaaacccga tgcccggctg ccgccaacac cgcttccacc gagtcggcgt caagacgtcc
    15481 ggcgctcgcc tcttggcgca gctggctcgc ggcagcgggt ggatcgaggg cggctcgaag
    15541 tggacgcggc tcgagcagtt gttgataagc caccgccgcg gcgagcagac ggtcctgtgc
    15601 agtcaattcc gcgccggcga gtcctcgggg aaagcccgaa ccatcgatcc gctcctcgtg
    15661 tttggtagcg agggacacca ccgactccag gccgccgacc ctgctcagta tccgtccggt
    15721 gagatacggg tacatccgta ctcgctccca ctcagcggta ctaagcgacg ccttcttttc
    15781 ccaaatctgg ttggacaccc ctagtctgcc gaggccgtgc acgaggcccg cacggtagag
    15841 ccaggtgatg tccgccgccg gcatccgcag gtgccgggcc gcggcggtca cgagatcggc
    15901 cacgctacgg gagtagccgg gagaacacgg acacttgaga tcaacgaaat ctgccatagc
    15961 cctgagtaac tcgtcgagct cgtcaccgtc gagaatgcgg ccacgatccg gcgcctgcgc
    16021 caaagcctca gcccaaacat ccatttcgag cagcccgtcg acgatcgcgg cggcgtcacg
    16081 ctcgaacacc tcagcgacct ccgggctgaa ttgcgtgcct cgacgggaac ggacaacctc
    16141 gagcgcccgt cccaggccac ccgtccgcag gtgcacctcg acgacatcgg ccaggcgaac
    16201 gatgcggatt tcgaccggaa tatcctcccc acgaacgccg ttcggcatcc cgcggccatc
    16261 ccagcgctcg aagatatacg ccagcgcctc cccaacctgc cggtccaggc ccatccgatc
    16321 ggagagaacc ccggcagaag tgcagtgcga ctggatcagc ttgccgacct ggttacgtgc
    16381 ggtgagcaga aaagaggccg agcggatgcc acgctcccat cctggtaagc cacgccccac
    16441 gtgactgatc atgaggcgca ggaacggtag cccggtcatg tccaccccgt aggtgtccgc
    16501 gcggaaggcg atgtcgtcgc caaacagcgc cgaaagctca tgggagtcgg cgtgacaacc
    16561 gatccaaccg accaggttgg cgtagtagac cgtcgcccgc tgttggtcgt cgaggcccat
    16621 acgctctgcg atgcgcgtcg cgatcaatga cgaccgaagc atgtgctcca tcggctgccc
    16681 cagaccgagg tcgatcgcca acgatattgc cgccagtaac tcggctctgc gcaggccttt
    16741 tttcgagccg tgcagcgtcc gggtcgggtg cacgacctta ttctgcacct gaacgtgagc
    16801 atattgacgc aaacgataag gtgcaggccc cgttacatgc gcccgaggca ggcgcgcatg
    16861 tcgtcggaca ttcaacgccg cggcggcccc gcggtcggat gtcaccgctc ggtcctgggg
    16921 ccgatatcca tcgtgtagca caccgcggtt ccgtccagga cgacagttcc gtcgtcacgg
    16981 gtgacggtcg tccgcagttc cgtgatcggt ttgtcggtgc gcgccgagat gacttcgacg
    17041 cggcccgtga tggtgtcacc gcagcgcacc ggcgccttga acgcccagtt gacattcagg
    17101 aacaccgtgc ccggacccgg caactcttcc gccacaacgg cattcaggat tgcgctggtg
    17161 accccgcctt ggacgacgat ttccccgaaa cgggacgcct tggctgcgtc ttcgtcatag
    17221 tgcaaagggt tgcggtcacc gctgatctct gtgaataggg cgatgtcgtt gtcaccgaca
    17281 gtgcgtgaga gctgcgctgt ctgacctacg aacggcgtcg tgtgggtaga gctggtggtc
    17341 atggttttcc tccttgagta ggtggtgttg aggatcgtcg agggccgaca tcgcgacggc
    17401 accgatcagg gggccgggtg caagaaacag gaacgcgtat tgccaaccga cgaggtctgc
    17461 gacgacgggc acgaattgaa tggtgacgac ggtcaacagg aaaccgattg cggtctgcgc
    17521 ggtcagagcg gtgccgacga accgggtgtc cgttgtctcg ctgagagcgg tggagaagac
    17581 gcccgagtct gcgatgacgg aagcacccca caccaacaga aagacgacga gaacgagtgt
    17641 gggggcggcg aagaatatcg gcgacgcgat gcagcaggtt ccgctggtca ccagggctgc
    17701 caccgccgct ggggggcgac caaagcggtc tgatgcccag ccgcccagaa ggcatccgac
    17761 agccccggcg actccgatgg cgacgaacgc gatgaatcca ttcgacgccg tgaccgcgtc
    17821 gcctcgcgcg ccctggccgg ccctgacgaa catcggcagc cacgtccaga gggcgtagag
    17881 ctcccacatg tgcccgaagt agccgagact gaccagccgc ggtccgcgct cggcgagtag
    17941 tgccagcgcg tagcgcgggt ttggggtgct cgcgcgggcg cccagatgtg ggccgggccg
    18001 aaccaccgct gaggcgagga cggcgccagt cgcggtcagt gccgccgcgg ccagcagcac
    18061 tgttctccag ggcagtggtc cgaggccact gatgaggtgg ggcagtgcgg agccgacggt
    18121 aagtgcggcg agcaggaccc cgaacgtgcg gcctcgatcg gtggatgcgg accatgaagc
    18181 catcagcttc atccccaccg gatacacgcc ggcgaggagc atcccggtga ggaatctcag
    18241 cgggactgcc gcaccgagac cggtggcaaa gaaggccagt gccgctgtgc aggccgctgc
    18301 gccaagggcg cttgcagcga gcaggttctg cggtgtggtg cggtcggcga ggttgaaaac
    18361 tgtcgaggtg atcgccccgg caacgaatcc gagttgcacc gacgcggtca gccagaccgc
    18421 cgccgtcgct ccgatacccc attctgatcg cagcgtgggc acgactgcgg tggccgagaa
    18481 ccagacactc aggccgaaca cctccacaag cgcgatgagt gcgcgttgct ggttggtccg
    18541 tgacagcatc attgcccaat tccattcagg cggcggacat ctcgtcgagc acgagcagcg
    18601 cgtcgacttc tgagcgcatg gcctggttca gctcgcgaag gcgcccgcgc agctcttcca
    18661 tatcccagct gtagctcgca atgcgctcgg tgatgtcgaa cagcggcaca gatagtcggt
    18721 ctcgggtgcg ctcgtagtcg tggagggtgt tgagctgtgc gcgcccgcca cggggtgcgt
    18781 cgaggaccgc gcgggcgagc agttccgcgt cgcgcaaggc gtcggtgatg ccgtgtgcgg
    18841 tgatcgggtc cttgaaatat gctgcgtcgc cgaccaacgc ccaacctggg cctgcggagc
    18901 cgcgaatcca gccgggctgg ccgggaaagc cgcgcagcgt gccgacgggt ggtgcatcgc
    18961 cgatcgcccg agccacctcg ggggcggcct cggtcagcag cgaccagaag gcggcgtcga
    19021 ggctggcgga tcgccgttgg gcgaactcgg cgctcgcagt gcccacccaa acgcacgcca
    19081 acccgtcgct cgtcggcacc acgcctgcgg tgactcccgg tcggtaagcc cagtgatagc
    19141 ggtcgcgcgg gaggcctggg aagtagcggt agaggatcgc gctggacgct gtgccgcggc
    19201 ggcggaatgg cgcgtcgacg cccttcgcga tcgttgagtt tcgcccgtcg gccccgatgg
    19261 tcagcggcgc gatggcgcga aaacgagttc cggtcccgcg ctcgatgccc tcgatgccgc
    19321 gcactctgcc gccgtcggtg agcaggcgcg tcacccgcgc cgacgtgcaa acctcgacgc
    19381 ctgccgcgtg ggcggcctcg agcagcagcg cgtcgaggac agttctgcgc ggggcgtaca
    19441 gcgcggatat gccaccgctc gggcggatgt cgacggtctc gatgtcgtcg ccgtacgcga
    19501 actcggtcga aacgatggcg ggggtgccgg cgtcgacgac cctgtcgagc agaccccagc
    19561 ggtgcagctg cagcacgccg ccgcgcatca gcgcatgggt ggacaaggtg tcggtgcccg
    19621 gccgggacgc ctcaatgacg agcacacgca tgccgccgcg ggccaggagc atcgcggtgg
    19681 ccgcaccgga acaccggccg ccgacgacga tcgcgtcgta gtggtgatcc atgagatgct
    19741 cctcagttcg cgtcgaggta gtgggcaagg tgtccgctca ggtctgccgc gtctgcggcc
    19801 gcgccgtcaa tcaaggtcga cttccggcgg cgcagaaatg gcatccccat caggtacagg
    19861 ccggggctgg cggtaatgcc gccgtcgtgg cggacatggc cctttctgtc gaacactccg
    19921 acgtcgagcc aggacaggtc cgggcgaaac ccggtcgccc agaggatcgt tcggatggca
    19981 cccgagctca ggtcgagtgt cagtggcgcg tgggtgggaa cggcggtcgc tgcgaaccgt
    20041 cgcggcggac catcagtgtc gaagcccgtc gcggtcgccc atccgtcgat ggtgtcgagc
    20101 agcctttcga gcttgaggtc ggcaagcgcg accatgttcg gcaacgagcc agagaattgc
    20161 gcgatcccgt ctctaatgcc caccaaccgt ccgaccagct gcacgccgag ctgactcagc
    20221 gaattcaagt cgactgtggc tttgtcgggt gaaccgatga gttgcatcga cggcaggttg
    20281 cgcgctcgga ttaggtcggg aatctcgtcg tagcgctcgt ccagcagtcc cgccgcatcc
    20341 atccaccaca agatgtcctt gcctcgatag gtgcgcggca ttcggacgtg ttccccgacg
    20401 gcgagcgtga cgtgccgacc ggagcggtgc acctccgcgg caatctggat cccgcttgcc
    20461 gaggcgccga cgacgagaac cccgccgtcg ggcagctggc tcggattgcg gtagtccacg
    20521 ggggtcaggg tggtgatgcc gacgggcacg ctgccctgta gcgcaggcac attgggaacg
    20581 gtgcttgcgc caggggcgag cacgaccgac cgcgcgtgcc acaactcctg gtcggtctgg
    20641 acgaggtagc ctcgctcgtc ctgcgccacc gatgtcacag tggtgttggc tcgcacgggc
    20701 gcagccgatt ccttcgcgta gttcgcgatc agctccacga cgtcgggtgc cgcaaggtac
    20761 ccgtcggggt cgtcgccttg gtacgcataa cccggcagcc gggtcatcca gttcggggtg
    20821 agcaatcgca gcgagtccca ccgctgtgtg cgccaggagt gcgccacctc accgcgttcc
    20881 agtacgacgt ggtcgatcga acggtcggcg aggcagcgac tcatggccag tccgcagtgc
    20941 ccggcaccca ccacgatggt ggttgtcttc agcatggtgg gcctcctgtt gaaacgtcgc
    21001 ccggtcagga cagttcgacg gtgacgtggg tggggttggt cagaatgtcg tagacggccg
    21061 agcgtttctg tgattgcgcg acaagggctt tcacttggtc gggagtagcg tcggcgtcga
    21121 tcttgtagct caccctgacg ccgctgaagc cgttgcggat gtggggatca gcaccaagaa
    21181 ttccgtggat gtcgtggtcg gcctcgatgg ttgccttcac cgaacgcaac tgaatcccgc
    21241 gctgctgcgc gatcgatgcg atgccggcgg taaggcagcc gcccagggcg accaacacgt
    21301 actcgaccgg ggtgatgcca ttgtcctgtg cggcgaacgc gagcggatgg tcgacgtcat
    21361 aggtaaacgt cgtcttgtgc tgttgctcct cgccgaaccc gtagaagggg tccacgctcg
    21421 agcgactatg ggtgccgccc acccaggaaa ccgttgtccg ccatcggaac tgggcgatct
    21481 cgggtgtgtc ggccaaggcc tcacgggcac cgagcagcgc ttctacgttg acgccgttgt
    21541 cgacagcggt atgcgttgtg gtcatgaact tctccaatct tggtgatcgg ttccatccgg
    21601 gaaaccggtc agatttctgt cacgccgcaa tgttcggccc cggagctggg accgcacatg
    21661 gggcacatgc ctcaaattcg ggccttatcg ccggcttggg cagccgccac gtcttcgggc
    21721 cggatgaagt tgaggcggat gcctcatgca ccgaaataga tgtcggtcaa ccatttccac
    21781 atggtcaccg tcaacattcc acgcgatacc gccccggcga tcgaccgtga tccctgggaa
    21841 aaggtgctaa cggacggtca tgcgtcgttg gtgagggcgc gacgcctatt tcgctatctg
    21901 ccctccgcgc cgcgctgcaa ggtgtgcaac aacccgtttg gcggccccgc cggacgcgtg
    21961 ctcgccgctg ccggcttcag cccctcgcgc aaaaatccca acctgtgcag tcggtgttgt
    22021 gatgcgctgc ccccaggcgg cgctgaggtt gacgtcgcgg tcctgttcgc cgatgttcgg
    22081 ggctcgacag cgctgggcca gcgcggcgcc gcgtcagact tcgcctcgtt gctcaaccgg
    22141 ttctacgtca cggcgacgca gacccttctt cgccatgacg cggtgatcga caaactcatc
    22201 ggcgatgaag tcatggcttt ctttgtccgt ggcatcagcg gcccccagta ccgacgccgc
    22261 gccgtccaag caggaatgga tttactcaaa gcggtcggtt acggcaccaa cgcgggccct
    22321 tcgctcgagg tgggcgtggc ggtcaatgcg ggcgtcgcct acgtgggcaa cgtcgggggc
    22381 tcggtcgtcg acttcaccgc acttggtgat ccggtgaaca tctcggcgcg gatgcaacaa
    22441 cacgctgcgg gcggggagtt gctcgtcgct gcgggcgtgt ccgatgacct catgacgaaa
    22501 atgccgcggc gcgtgctgaa cctgcggggg cacgcggagc cgatcgacgc gttcgtcgtg
    22561 ggggcctgag gctactcagg cgacgtgttc gcggcggcgc acgccctcat gcagcggcag
    22621 gtcaggatcg atcttgatgc cgagcagttc acaggtgcca ttgatggagc cgaccatgat
    22681 cgtcgtcagg tgcgcgacga actcgtcggg cggcatccgg cgtgggctgt ccggctcggt
    22741 gcccaaccac cagtcggtgg ccgaggcgat agacccgaac gcggagtagg cggcgagttc
    22801 gagcgccgcg gcgtcgagct ccatgtcccg cagctcgttg tcgaacatgt tggccatcgc
    22861 caacgtgatc tcgcggcctt cgttgagcgc gcgcatcgtc gactcgctct gctcggcgaa
    22921 gcggccctgg atcaggaagc gcaggacgtt cgggtgctcg tcgaccagcc ggacatactg
    22981 ctcgacactg cggcggatca cctcgcgcgc cgggtcgctg gacagattga tggacgggaa
    23041 gatcgccgcc cacaacatgt cccgcagccg ctcgccgacc gcctggaaca ggtctgactt
    23101 gtcggtgaaa tgccggtaaa tcttcggctt cgcggtgccg gcctcttcgg cgatctcacg
    23161 caggctcaat tccggtccga gctggtcgat cgccctgaac gcggcgtcga cgatctccga
    23221 ccgcaccttc ttgcgatgct cgcgccagcg ctcgctgcgg gcgtccactt tgacacccgg
    23281 ctcgctgctg gaacgtgacc tcgaccctcg tcgcacccga ttactgtacc gcccggacgt
    23341 cgctgacctg ctcagaccgc ctcgcaacac ggagttttgc ggcgctggcc aggcgaattt
    23401 gcttgccagg aaccgacccg tcaccctggg taatattcgt gcgacatccc atgaacgaga
    23461 gagacgtatg acgcagcgat acgacctggt catcgcgggt ggcgggccat cgggctcggc
    23521 cgccgcgtgg caggccgcgc aaaccggcgc caaggttgtg gtcctggaca aggccgagtt
    23581 cccgcgggac aagccgtgcg gcgacggcct gaccgcgcgc gccaccagct atctgcagaa
    23641 gatgggtttg gccagcgagg tcgcccagtt tcaccgcgtc aaccgggtca cggtgttcag
    23701 cccgagccag tgggagctgt cgttccccaa gcggcccggc atgcccgacc acggccacac
    23761 cgtcagccgc accgagctgg acacgctgtt gctcaagcac gcggagtcgg cgggggccga
    23821 ggtgcggcaa tcggccgagg tgaccgcgcc gatcatcgag aacggtcgag tcgtgggtgt
    23881 gacgctgaag agcggcgaga aggtgtacgg cgacgcggtg atcgccgccg atggcgcgta
    23941 ctcccccatc aagcgggcgc tgaagatcga ttcggagtac aacggctact cggcgatcgc
    24001 gatccgctcg gagatgcacg ccaaccggcc ggattccgac agcctcgaca tctatttgaa
    24061 gctggagttc cagggcgacc agctcccggg ctacggctgg gtgttcccga tgggcggcgg
    24121 gcggttcaac atcggcctgg gctacgtgaa cagctacaag aactggcagt cgatcaacgc
    24181 gacgcagttc ttgggcgagt tcctgcgaac gctgccgcgt gattgggagc tgccaccgat
    24241 cgaagagctg aagaagaaca agagcgtgcg ggcgtggcgg ctgccgatgg gcttcaccgc
    24301 gtggccgccg tggcgaccgg gcgtgctgtt cacgggcgac tcgttggggg cgggcaagcc
    24361 ggcgtcgggc gcgggtatct ccaaggcact cgaatccggt ttggccgcag gcgaatgcgc
    24421 gatcgcggca ctgcagaacg gcggtcccga cgacttcacc aactacgcgc agaggatgga
    24481 agcggcgtgg ggcaaggagt accggcgcgg ccgctacttc cacaagctgc tcggccagcc
    24541 gcggctcgcg aatgcaggta tcaagctgat cgacaacgct gcgttccgcg accggatgct
    24601 gaaagcgttg tacaagaagg cgcaaggtcc gcagcacacc tacaagtagg attcccgctc
    24661 accggcgtaa acaggtgggc ccacggcgta gtttgacgtc acatggccac gcaaccccgg
    24721 ggggtcgcca tggaagtcaa aggcgccgac ggcactccgc tgacctatga cgtgagcggc
    24781 gacggcgaac cgctcgtgct gattcacggc tcatggggcg aacgccagac gtgggggctc
    24841 gtaatccccg cgttggcgga gtccttccgt gtgatcagtt atgaccggcg cggccacggc
    24901 gaaagcagcg cgtcgcccga tatcggaacg gtgcatgacg acgtcgccga cgtcgttgcg
    24961 gtgatcgacg agttggcaga cggtcgcgcg catctcgttg gcaactccta cggggcatgt
    25021 atagccctgc gaatggcggc cgcccaccca gaacgcctcg cgaggatggt ctgccacgag
    25081 ccccctatgt tgggagtgct cgaagcagac gacgatggca agccgatcgc cgacgaggag
    25141 cgaagaaagc ttggcgaagt gcggcagcgt ctcgagcgag gggactacgc cgacgctgcg
    25201 gagtacttcg tcgaacgagt ggctttcgga cctggggcgt ggtcggagct tccgccgcct
    25261 ctgcagcaga tgttcgtcaa gcacgcaccg acgttcctcg gtgagctgcg cgacggcgac
    25321 gcgcttggcg ccgatctgga atctttgcga caactcaaga cccctatcct cctcacgcac
    25381 agcccggaca gcccggcgtt cttcgcgccg atcgtcgaac ggctcgcgat cctgcttcct
    25441 aacgcccgaa ggaggctgct gtctgatatc gggcatgtgc cacatatgac gcatccggac
    25501 gagtacgcgc cggtggtgct ggactttctg ctcgagcagc acggatgatg tccgccgcac
    25561 gaagcggccc ctaagcccct atcggtggca gcgccggcaa cccgcaagcg aatgaccgag
    25621 aggcgggagt ccaccccgtt ttggcgttga ccgcttcgca tcaggcttag cggcatgaac
    25681 atcaaaggac ttctggccac cgccgcgatg acggccggcc tggccatcgg actcgccgcg
    25741 aacgcgaatg cggcgcccgt cagcgacgat ttctgggagt gcgcagcgga tcatccggac
    25801 gacatcggcg tctgctgcgt cttctacgga ggcgactacg acgagcggac cggcgagtgc
    25861 tggatagacc acgaagcgaa gctagaggag gccaaccccg gccccagcgt tccgccgaag
    25921 ca
//
LOCUS       DASRJA010000300         2670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2294307,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000300 DASRJA010000000
VERSION     DASRJA010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2294307"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..797)
                     /locus_tag="VFC01_13145"
     CDS             complement(27..797)
                     /locus_tag="VFC01_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HZN80613.1"
                     /translation="MTGFAEIAAAGPVLLAIGVSVLAGLVSFASPCVVPLVPGYLSYL
                     AAVVGVEEQPGHVAVRTARLRVAGAALLFVAGFTAVFVLGTVAVLGMTTTLIANQLLL
                     QRIGGVITILMGLVFVGFIPALQREARFTPRQISTLGGAPLLGAVFGLGWTPCLGPTL
                     TGVIAVASATDGATVARGVALVVAYCLGLGIPFVLLAFGSARAVQGLGWLRRHTRDIQ
                     IFGGVLLILVGAALVTGLWNDFVSWVRDVFVSGVTLPI"
     gene            complement(794..1390)
                     /locus_tag="VFC01_13150"
     CDS             complement(794..1390)
                     /locus_tag="VFC01_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZN80614.1"
                     /translation="MKRLVALASAAIVALAGCSTGDDAVAQGGTFEFVAPGGKTDIFY
                     DPPESRGRPGQLTGPELMDPAKTISLDDFAGKVVVINVWGQWCGPCRTEITQLQKVYD
                     ATRKQGVAFLGIDVRDNNRDAAKDFIVDRRVTFPSIYDPPMRTMIAFGGKYPTTVIPS
                     TVVLDRQHRVAAVFLRELLAEDLQPVVERLAAEPEPKT"
     gene            complement(1387..1995)
                     /locus_tag="VFC01_13155"
     CDS             complement(1387..1995)
                     /locus_tag="VFC01_13155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZN80615.1"
                     /translation="MTEKTIVHVMRHGEVHNPDKILYGRLPDYHLSERGRAQAQAVAD
                     WLAQRDVVYVVASPLERAQETAEPLATRLGLPIDTDEDLIESMNIFQGQRVSPGDGAL
                     RDPRNWWYLRNPRTPSWGEPYKEIAARMTAALHRARIKAAGHEALCVSHQLPVETLRR
                     AMTGASLHHFPTRRMCNLASLTSFYFHGDSYVGWGYSELAGQ"
     gene            complement(1992..>2670)
                     /locus_tag="VFC01_13160"
     CDS             complement(1992..>2670)
                     /locus_tag="VFC01_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN80616.1"
                     /translation="PGNMGTVPPLPGYNAALRRITAEHGALLILDEVMTGFRVSRSGW
                     YGIDPVDADLFTFGKVMSGGLPAAAFGGRAEVMERLAPLGPVYQAGTLSGNPVAMAAG
                     LATLRTADDTAYAALDANANRLAALLVTALTEAGVAHQVQRAGNMLSVFFTDEPVHDF
                     AAARASETWRFPSFFHALLDAGVYPPPSAFETWFVSTVLDDEAFERIADALPGAARAA
                     AEAKKPA"
BASE COUNT          473 a          914 c          884 g          399 t
ORIGIN      
        1 tgcgtctgct cgcggggaaa cgttcgtcat atcggcagcg tcacgccgct gacgaacacg
       61 tcgcgcaccc aggacacgaa gtcgttccac agcccggtca ccagcgcggc gcccacgagg
      121 atcagcagca cgccaccgaa gatctggatg tcgcgggtgt gccgccgcag ccagccaagg
      181 ccctgcaccg cgcgcgctga gccaaaggcc agcaacacga acggaattcc caaccccagg
      241 cagtacgcga cgaccagcgc gacaccgcgg gccaccgtcg cgccgtcggt cgccgacgcc
      301 accgcgatca ccccggtcag cgtcgggccc aggcacggtg tccagcccag tccgaagacc
      361 gcgcccagca gcggtgcacc accgagagtc gagatctgcc gcggcgtgaa gcgggcctcg
      421 cgctgcagcg ccgggatgaa gccgacgaac accaacccca tcaggatcgt gatcacgccc
      481 ccgatgcgtt gcagtagaag ctgattggcg atcagcgtcg tcgtcatgcc gaggaccgca
      541 acggtgccga gcacgaagac ggccgtgaag cccgcgacga acaacagcgc cgcgccggca
      601 actcgcagcc gcgcggtgcg caccgcaaca tgtcccggct gttcttccac accgacgacc
      661 gccgcgaggt aggagagata gcccggcacc agtggcacca cacacggcga ggcgaacgaa
      721 accagtccgg ccagcacgga tacgccgatc gcgagcagta ccggtccggc ggcggcgatc
      781 tcggcgaacc ccgtcatgtc ttcggctccg gctcggccgc aagcctttcc acgaccggct
      841 gcagatcctc ggcgagcagt tcacgaagga acaccgcggc gacccggtgc tggcggtcga
      901 gcaccacggt ggacgggatg acggtggtgg ggtacttgcc gccgaacgcg atcatggtgc
      961 gcatcggcgg gtcgtagatc gacggaaacg tgaccctgcg gtcgacgatg aaatctttcg
     1021 cggcgtcgcg gttgttgtcg cggacgtcga tgccaagaaa tgcgacgccc tgcttacgtg
     1081 tggcgtcgta caccttctgc agctgagtga tctcggtgcg acacggtccg caccattgcc
     1141 cccacacatt gatcaccacg accttgccgg cgaaatcgtc gagcgaaatc gtcttggcgg
     1201 ggtccatcag ctccgggccg gtcaactggc ctgggcggcc acgactttcg ggtggatcat
     1261 aaaagatgtc ggtcttaccg ccgggtgcca cgaactcgaa ggtgccgccc tgcgcgacgg
     1321 cgtcgtcacc ggtggagcaa ccagcgagcg cgacgatcgc cgcgcttgcc agggccacca
     1381 gccgcttcac tgcccggcca gctccgagta tccccagccg acataggagt cgccatggaa
     1441 gtagaaggac gtcagcgagg ctaggttgca catgcgtctg gtcgggaagt gatgcagact
     1501 ggcacccgtc atcgcccgtc ggagcgtttc gaccggcagc tgatggctga cgcacaacgc
     1561 ctcatgaccc gccgccttga tccgcgcccg gtgcaatgcc gccgtcatcc gcgccgcgat
     1621 ctccttatat ggttcgcccc acgacggagt ccgtggattc cgcagatacc accagttgcg
     1681 cgggtcgcgc agcgccccgt cgccgggcga gacacgttga ccctgaaaga tgttcatcga
     1741 ctcgatcagg tcttcgtcgg tgtcgatcgg gagaccgagt cgcgtcgcga gcggctccgc
     1801 ggtctcctgt gcgcgctcca gcggcgaggc gaccacatag acgacgtcgc gctgcgccag
     1861 ccagtcggct actgcctgcg cttgcgcgcg gccacgctcg gacaggtggt aatcgggcag
     1921 tcggccgtac aagatcttgt ccgggttgtg cacttcgccg tgccgcatca catgcacgat
     1981 cgtcttctcg gtcatgccgg cttcttcgcc tccgcggcgg cgcgcgcggc gccaggtagg
     2041 gcgtcggcga tccgttcgaa ggcctcatcg tccagaacgg ttgagacgaa ccaggtttcg
     2101 aacgcactcg gcggggggta gaccccggcg tcgagcagcg cgtggaagaa ggatgggaag
     2161 cgccacgtct cgctcgcgcg ggcggccgcg aaatcgtgca ccggctcgtc ggtaaagaac
     2221 acgctgagca tgttgccggc gcgctgaacc tgatgtgcca caccggcttc cgtcaacgcc
     2281 gtgacgagga gcgcagccag ccggttggcg ttcgcgtcaa gcgcggcgta tgcggtgtcg
     2341 tcggcagtcc gcagcgtcgc cagtccggcg gccatggcga ccgggttccc agacagagtg
     2401 cccgcctgat acaccgggcc tagcggcgcg aggcgctcca tcacctcggc gcggccgccg
     2461 aacgcggctg cgggcagccc gccgctcatc accttgccga aggtgaacag gtcggcgtcg
     2521 acgggatcga tgccgtacca accggatcgg ctgacccgga agccggtcat cacttcgtcg
     2581 aggatcagca gtgcgccgtg ctcggcggtg atcctgcgca gcgcggcgtt gtaaccggga
     2641 agcggcggca cggtgcccat gttgcccggg
//
LOCUS       DASRJA010000301         5095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3462799,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000301 DASRJA010000000
VERSION     DASRJA010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3462799"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFC01_13165"
     CDS             <1..232
                     /locus_tag="VFC01_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80617.1"
                     /translation="SSGGSGGGGAASVDTVTVAPSTVTNTETDIATTVQTSVQTSVAT
                     ATETVTATPTYQPRDQFPRNDNRSQFGSQQGH"
     gene            322..981
                     /gene="phoU"
                     /locus_tag="VFC01_13170"
     CDS             322..981
                     /gene="phoU"
                     /locus_tag="VFC01_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZN80618.1"
                     /translation="MRTTYYEELSSLSAQVGEMCGRAADAMEHATQALLGADLSVAEQ
                     VIADHEHIVAMSKRAEACAIKLLALQQPVAGDLRTIVGAIHVGADIERMGALAVHVAS
                     ISRLRHPECALPDDVRASFAEMGRRAVELARTAQEVLSSGDPDKAARLQDQDDAVDAE
                     HRHLFTLLIDQKWQDGVCSAVDVALLGRFYERFADHAVEIGKRVVFETTGGMPDHKQM
                     A"
     gene            1044..1424
                     /locus_tag="VFC01_13175"
     CDS             1044..1424
                     /locus_tag="VFC01_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN80619.1"
                     /translation="MDQNTYDKGREVRAAVLGEAYVNAATADADDFNKPFQDLVTEYC
                     WGAVWGREELPLKTRSMLNLAMISLLNRPQELRTHIRGALTNGVTRDEIREVFMQVAV
                     YAGVPAAVDSFRIARQTFAELDEG"
     gene            1430..2311
                     /locus_tag="VFC01_13180"
     CDS             1430..2311
                     /locus_tag="VFC01_13180"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80620.1"
                     /translation="MDIGFIGLGHMGFPIARRMVAAGHNVVAFDTRAEVVETLVGARA
                     AASARDVADNAETVMASLPSPQASRDVATAVAEGSRIKRFVDFSTVGRQTAIEVHVLL
                     ARRGIVAIDSPVSGGVSGANSGTLAVMVSAPRDDFELLTPVYEAIGRAFYLGDKPGSA
                     QTMKLVNNLLAATGLIATCEAVAMGVKAGLDAATMVEVLNAGSGGTNASRDKFPRSIL
                     PRTFDYGFATGLMVKDVRLYVAEAKALGVSMEVAEAVARLWETVNRELGPESDFTAAI
                     KPIERAAGVVVGGPQRD"
     gene            2356..3462
                     /locus_tag="VFC01_13185"
     CDS             2356..3462
                     /locus_tag="VFC01_13185"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN80621.1"
                     /translation="MQTYKVAVWATGGVGRFAIRTIVDRPNYELVGAWVHSASKDGED
                     IGTLAGIAPIGVAASRDETAVLDGDADCVVYAAPAGSRPKEALADLCRILAAGKNIVT
                     TSLPGLVYERGSLSERYLDAIRQAAQAGSSSIFSSGIEPGFGCDLFPIALMTMSHKVY
                     SVRGIEITDYSRYPVEFDMRELFGFGQPLDYQGGLKSPGVLTWGWGAAVTMVADALGV
                     QLDEIRESCEFLPTPRPLETACGVIEAGTVGATYAKCIGVVDGQEVITIEHVDRMADD
                     LAPDWPKSRTGGIDGTWRVIVDGEPSFDAEFEVGFRDGESSSDQGLLATGMRAVNAIP
                     WVCDAAPGVVDALHLPLTSPLGALHPHRDGIPAF"
     gene            3464..4321
                     /locus_tag="VFC01_13190"
     CDS             3464..4321
                     /locus_tag="VFC01_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN80622.1"
                     /translation="MGALDGKIAVVTGTSRGVGVGIAHELLRAGATVIGCSRSKLDGL
                     PGVEPEWVPWSAQLVCDQGDYRSVDALVSQVAETYGRIDILVNNAGGTVPTPNVEDVP
                     ALVAQIQGAPRSDDDFERTVLFHSFAIQMNLISPLWFAIRVYRQMRGQDGTGSIINIS
                     SGAGHPAGSPTLVSYGAAKAGLNHLTRSLAEEWGPRVRVNALALGPTMTENFRSFVLP
                     KDDPDGAQYFRNVPLKRAGKPAEVGRACVFLCSGAADFINGTTIEIDGGMLPGVLYEA
                     GLKTITDLL"
     gene            4331..>5095
                     /locus_tag="VFC01_13195"
     CDS             4331..>5095
                     /locus_tag="VFC01_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN80623.1"
                     /translation="MRRVIQFSTGNVGRHALRTLIERPDLELVGVHASGAEKVGRDAA
                     ELCGFTTPTGVVATDDIDALVALGADCVVYTSQAEMRPTEAIDEISRFLAAGTNVVGT
                     SMVWLVAPEQADDWLRDPLEKACKAGDTSLYINGIDPGFSGDTLVHTALSLSARATAI
                     TVQEIFDYGSYDDAEFTGVSMGFGTTPDHTPIMFQPGVLASLWGGQVRSLADGLGVEL
                     NDVRERHETWVTPEPIECTMMTVPPGGVAAVRFAVEG"
BASE COUNT          911 a         1649 c         1702 g          833 t
ORIGIN      
        1 ttcgtcgggc ggctcgggcg gcggcggtgc agcctcggtt gacaccgtca ctgtcgcgcc
       61 cagcacggtc accaacaccg agacggatat tgcgacgacg gtccagacct cggttcagac
      121 gtcggtcgcg acggccaccg agaccgtcac cgccacgccg acctatcaac cgcgcgacca
      181 gttcccccgc aacgacaacc ggtctcagtt cgggtctcag caaggtcact gatgcgcggc
      241 ggattagact ggatcgcgcg cgggtaccag gtagtgctgg ctagcagccg gctggtgccc
      301 agcagcggag gtttcagaat catgcggacg acatattacg aggaattatc ctcattgtcc
      361 gcccaggtcg gcgagatgtg tgggcgggcg gccgacgcaa tggagcacgc gactcaggca
      421 ctgctgggtg cggatctctc ggtggcagag caagtcatcg ccgaccacga gcacatcgtg
      481 gcgatgagca agcgcgccga ggcctgcgcg atcaaactgc tcgccctgca gcaaccggtc
      541 gccggcgact tgcggacgat cgtgggcgcg atccacgttg gcgccgatat cgagcggatg
      601 ggtgcgctgg ccgtgcacgt cgccagcatc tcgcggctgc ggcatccgga atgcgctcta
      661 cccgacgatg ttcgcgcctc attcgcggaa atgggccgac gggcagtcga attggcccgg
      721 actgcgcagg aggtgctgag ttcaggcgat cccgacaagg cggcccgact gcaggatcag
      781 gatgatgccg tcgacgcaga gcatcgccac ctcttcacac tgctgattga ccagaagtgg
      841 caggacggcg tgtgttccgc ggtcgatgtg gcgctgctgg gccgcttcta cgaacgcttc
      901 gccgaccacg ccgtcgagat cggcaagcga gtggtgttcg agacaaccgg cggaatgccc
      961 gaccacaagc agatggccta actcagccgt caccatcctg gcctgacgta cgatccctcc
     1021 tcgagaagaa tcgaggaaac acgttggatc agaacactta tgacaagggg cgcgaagtcc
     1081 gggcggcggt gctcggcgag gcatatgtaa atgcggcgac ggcggatgcg gatgacttca
     1141 acaagccgtt ccaggatctg gtaaccgagt actgctgggg cgcggtgtgg gggcgcgagg
     1201 aattgcccct caagactcgc agcatgctga atctggccat gatctcgcta ctcaaccggc
     1261 cgcaggagct acgcacacat atcaggggcg cgctgaccaa cggcgtgacg cgcgatgaaa
     1321 tccgcgaagt cttcatgcag gtggcggtct acgccggtgt tcccgctgcc gtcgacagct
     1381 tccgcatcgc ccgccagaca ttcgccgaac tggacgaggg ctagctgtcg tggacatcgg
     1441 gtttatcggc ttgggccaca tgggatttcc catcgcaaga cgcatggtgg cggccggcca
     1501 caatgtcgtc gcgttcgaca cgcgtgccga ggtggtggaa accctcgtcg gagcgcgggc
     1561 cgcggcgtcc gctcgggatg tcgccgacaa tgcggaaacg gtgatggcca gccttccgtc
     1621 gccgcaggct tcccgtgacg ttgcgactgc cgtcgctgag ggttcgcgaa tcaagcggtt
     1681 cgtcgacttc tcgacggtgg gtcggcagac cgcgatcgag gtgcatgtcc tgctcgcccg
     1741 gcgcggaatc gtcgcgatcg acagcccggt cagcggcggg gtcagcggtg ccaactcggg
     1801 gacgcttgcg gtgatggtgt ccgcaccgcg agacgacttc gagctgctga cgccggtgta
     1861 cgaagcgatt ggccgagcgt tctatctggg cgacaagccc ggctcggcgc agacgatgaa
     1921 actcgtcaac aatttgttgg cggccaccgg cctcatcgcg acgtgcgagg cggttgcgat
     1981 gggcgtcaaa gcaggcctgg acgcggcgac gatggtcgaa gtcctcaacg ccgggtctgg
     2041 cggcaccaac gccagccgtg acaagttccc gcgctcaatc ctcccgcgca ccttcgacta
     2101 cggcttcgcc acagggctga tggtgaaaga cgtgcgcttg tacgtcgcgg aggccaaagc
     2161 actcggcgtg tcaatggaag tcgccgaggc ggtggcgcga ctgtgggaaa cggtgaaccg
     2221 cgagctcggc ccggagtcag acttcaccgc ggccatcaag ccgatcgaac gggccgcggg
     2281 cgtggtggtg ggcgggccgc agcgagactg acggaaacgg ccccgcaaaa acattgagtg
     2341 gtttatggtt tgcgcgtgca gacctacaaa gtggcggtgt gggcgaccgg tggcgtcggg
     2401 cgattcgcga tccgcacgat cgtcgaccgg cccaattacg agctggtagg cgcctgggtg
     2461 cattcggcga gcaaggacgg cgaggacatc ggcacgctgg ccgggatcgc accgatcggc
     2521 gtggccgcca gccgcgatga gacggctgtc ctggacggcg acgcggactg cgtcgtctac
     2581 gcggcgcccg ccggctcgcg accgaaagag gcgctggccg acctctgccg catcctcgcc
     2641 gcgggcaaga acatcgtcac cacgtcactc ccaggcctgg tgtacgagcg cgggtcgctg
     2701 tcggaacgct atctcgacgc gattcgccaa gcggcgcagg ccggttcgag ctcgatcttc
     2761 tcctccggca tcgaaccggg cttcggctgc gacctgttcc cgatcgcgct gatgacgatg
     2821 tcgcacaagg tgtactccgt gcgcggcatc gagatcaccg actactcgcg atacccggtc
     2881 gaattcgaca tgcgcgagct gttcggattc ggccaaccgc tggactacca gggcggcctg
     2941 aaaagccccg gcgtcctgac gtggggctgg ggcgccgcgg tgaccatggt ggccgatgca
     3001 ttgggtgtcc agctcgacga gatcagagag tcctgcgaat tcctgcccac cccacggccg
     3061 ctcgaaaccg cctgcggcgt catcgaagcc ggcaccgtcg gcgccaccta cgcgaaatgc
     3121 atcggcgtcg tcgatggcca ggaggtgatc accatcgagc acgtcgaccg gatggccgac
     3181 gacctcgcac cggactggcc gaagagccgc accggcggca tcgacggcac gtggcgcgtc
     3241 atcgtcgacg gtgaaccgag cttcgacgcc gaattcgagg tcggcttccg cgacggcgaa
     3301 agctccagcg atcaaggcct gctcgccacc ggcatgcgcg ccgtcaacgc catcccgtgg
     3361 gtatgcgacg ccgccccggg tgtcgtcgac gcgctgcatc tgccgttgac ctcgccgctg
     3421 ggcgcactgc atccacatcg cgacggcata ccggcgttct gacatgggcg cactcgacgg
     3481 caagatcgcg gtcgtcaccg gcaccagcag gggagtcggc gtcggcatcg cgcacgaact
     3541 gctgcgcgcg ggcgccacgg tcatcggctg ctcgcgttcc aaacttgacg ggctacccgg
     3601 cgtcgaacct gaatgggtgc cgtggtccgc ccaattggtt tgcgaccaag gtgattaccg
     3661 ctccgtcgac gcactggtct cgcaggtggc cgagacgtac ggccgcatcg acatccttgt
     3721 caacaacgcc ggcggcacgg tgcccacccc gaacgtcgag gacgtgcccg cgctcgtcgc
     3781 gcagattcag ggcgcaccgc gcagtgacga cgactttgag cgcacggtgc tgtttcactc
     3841 gttcgcaata cagatgaatc tgatcagccc gctatggttt gccattcggg tgtatcggca
     3901 gatgcgcgga caagacggca ccggctcgat catcaacatc tccagcggcg ccggccaccc
     3961 cgcagggtca ccgacgctgg tgtcctacgg tgcggccaag gccggcctga accacctgac
     4021 ccgttcgctg gccgaggagt gggggccacg cgtccgcgtc aacgcgctgg ccctcggccc
     4081 gaccatgacc gaaaacttcc gcagcttcgt gctgcccaag gacgacccgg acggcgccca
     4141 gtatttccgc aacgttccac tcaagcgcgc gggcaagccc gccgaagtcg gccgagcatg
     4201 cgtgttctta tgcagcggcg cagccgattt catcaacggc accaccattg agatcgacgg
     4261 cggcatgctg cccggcgtgc tgtacgaagc cggcctcaaa accatcaccg atctgctcta
     4321 ggggacacgc gtgcgacgag tcatccagtt ctccaccggc aacgtgggcc gccacgcgct
     4381 gcgtacgctg atagagcgcc ccgacttgga actggtcggc gtgcacgcca gtggcgctga
     4441 gaaggtgggc cgcgacgccg ctgaactgtg tgggttcacg acaccgacag gggtggtggc
     4501 caccgacgac atcgacgcgc tggtcgcgtt gggggccgac tgtgtggtgt acacgtcaca
     4561 ggccgagatg cgcccgactg aggccatcga cgagatctcc cggttcctgg cggcgggcac
     4621 caatgttgtt ggcacctcaa tggtctggct ggtcgcgccc gagcaggccg acgactggct
     4681 gcgcgacccg ctggagaagg cctgcaaagc gggcgacacc tcgctgtaca tcaacggcat
     4741 cgaccccggc ttctccggtg acacgctggt gcacaccgcg ctgagcctgt cggcgcgggc
     4801 caccgccatc accgtgcagg agatcttcga ttacggcagc tacgacgacg ccgaattcac
     4861 cggtgtcagc atgggattcg gcaccacgcc cgatcacacg ccgatcatgt tccagcccgg
     4921 agtcctagcg tcgctttggg gtggtcaggt cagaagtctc gctgacggcc tcggcgtcga
     4981 gctcaacgat gttcgcgaac gccacgaaac atgggtgacg ccagaaccca tcgagtgcac
     5041 gatgatgacc gtgccaccgg gaggggtggc cgccgtccgg ttcgcggtcg agggt
//
LOCUS       DASRJA010000302        12517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_231742,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000302 DASRJA010000000
VERSION     DASRJA010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12517
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231742"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VFC01_13200"
     CDS             <1..1118
                     /locus_tag="VFC01_13200"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZN80624.1"
                     /translation="PPSWRPDLREPADLVEEVLRLEGLEIIPSVLPQAPAGRGLTAIQ
                     KRRRAIGKSLALNGYVEILPTPFLPAGVFDQWGLPADDPRRNTTHVLNPLESDRPALA
                     TTLLPGLLEALGRNVSRGAVDVALYAIQQVVEPTSDTRAVDRIPNNRRPTDEEIAVLD
                     ASLPVQPQHVGVVLAGLREPAGPWGPGRPVEAHDAFEAVRVIGRAAGVELTLRPAQHL
                     PWHPGRCAEVLVGDVVVGHAGQLHPAVVERSGLPKGTCAVELDLDAIPLTEALAAPAV
                     SPFPAVFQDVSLIVGADVAAQSVVDAVREGAGELLEDVRLFDVYTGPQIGDGRKSVTL
                     ALRFRAADRTLTEDEASAARDEAVKSAADRVGAIQRA"
     gene            1217..2266
                     /gene="argC"
                     /locus_tag="VFC01_13205"
     CDS             1217..2266
                     /gene="argC"
                     /locus_tag="VFC01_13205"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZN80625.1"
                     /translation="MQNRCMTSVAVAGASGYAGGEILRLLLGHPAYADGRLTIGALTA
                     AASVGTTVAEHHPHLLPLAGRVLEATDIDVLAGHDVVFLALPHGHSAALAEQLAPDTV
                     VIDCGADFRLTDSAAWEKFYGSAHAGSWPYGLPELPGARDRLRDAKRIAVPGCYPTAA
                     LLALWPAVAEDLIEPAVTVVAVSGTSGAGRAAKADLLGSEVIGSARAYNIGGKHRHTP
                     EIAQGLKALTDKDVTVSFTPVLIPASRGILATCTARTGTPLSQLRAAYEKAYDAEPFV
                     HLLPEGQLPKTGAVIGSNAAQLAVAVDEDAETFIAIAAIDNLVKGTGGAAMQSMNLAL
                     GWPETEGLSIVGVAP"
     gene            2263..3477
                     /gene="argJ"
                     /locus_tag="VFC01_13210"
     CDS             2263..3477
                     /gene="argJ"
                     /locus_tag="VFC01_13210"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HZN80626.1"
                     /translation="MTDITETTRLVRTQGVTAPAGFRATGIAAGIKASSALDLALVFN
                     EGPDYAAAGVFTRNKVKAAPVLWSQQVLTTGRLRAVILNSGGANACTGPQGFQDTHAT
                     AEAVAAALSDWGTETGAIEVAVCSTGLIGDRLPMDKVLAGVTEVVHEMAGGLTGGEEA
                     ARAIMTTDTVPKQVALHHSNKWTVGGMAKGAGMLAPSLATMLTVITTDAVADAAALDH
                     ALRKATALTFDRLDVDGSCSTNDTVLLLASGASEITPSQTELDDAVHRVCDDLCAQLQ
                     ADAEGVTKRVVVTVTGAASEDDALTAARVVARDSLVKTALFGSDPNWGRVLAAVGMAP
                     VTLDAERISVSFNGSPVCIDGTGAPGARDVDLSDPEIDVTIDLGVGTQWASIRTTDLS
                     HGYVEENSAYSS"
     gene            3474..4346
                     /gene="argB"
                     /locus_tag="VFC01_13215"
     CDS             3474..4346
                     /gene="argB"
                     /locus_tag="VFC01_13215"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZN80627.1"
                     /translation="MTLATPVKAQVLAAALPWLKQMHDKIIVVKYGGNAMTDDVLKSA
                     FAADMVFLRNCGIHPVVVHGGGPQISSMLKRLGIEGDFKGGFRVTTPEVLDVARMVLF
                     GQVGRELVNLINAHGPYAVGVTGEDAHLFTAVRRSVTVDGVATDIGLVGDVEKVDACA
                     LRDLIGAGRIPVVSTIAPDTDGVVHNINADTAAAALAEALGAEKLVVLTDVEGLYTSW
                     PDRDSLVSEIDTAALTQLLPTLETGMVPKIEACLRAVSGGVPSAHVIDGRVEHCVLVE
                     LFTDEGTGTKVVSA"
     gene            4343..5512
                     /locus_tag="VFC01_13220"
     CDS             4343..5512
                     /locus_tag="VFC01_13220"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HZN80628.1"
                     /translation="MTLQQRWEAVMMNNYGTPPLALASGDGAVVTDVDGKSYIDLLGG
                     IAVNILGHRHPAVIDAVTRQLNTLGHTSNLYATEPGIALAESLVGLLGADARVFFCNS
                     GTEANEVAFKITRLTGRTKLVAAHGAFHGRTMGSLALTGQPSKQQPFAPLPGDVTHVP
                     YGDVAALEAAVDDATAAVFLEPIMGEGGVVVPPAGYLVAAREITSKHGALLVLDEVQT
                     GMGRTGAFFAHQHDGITPDIVTLAKGLGGGLPIGACLAVGTTGDLLTPGLHGSTFGGN
                     PVCTAAALAVLKVLADDDLIGRADVLGKMLSHGIESLGHPLVDYVRGRGLLQGVVLTT
                     EVAKPVESAAREAGFLVNAAAPDVIRLAPPLVITEGQIDDFLKALPGVLDKGTQS"
     gene            5509..6435
                     /gene="argF"
                     /locus_tag="VFC01_13225"
     CDS             5509..6435
                     /gene="argF"
                     /locus_tag="VFC01_13225"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZN80629.1"
                     /translation="MTRHFLRDDDLTPDEQAEVLALAAELKKDPFSRRPLDGPRGVAV
                     IFDKNSTRTRFSFEIGIAQLGGHAVVVDGRSTQLGREETLEDTGRVLSRYVDAIVWRT
                     FAQERLTAMACGSSVPIVNALSDEFHPCQVLADLQTLAERKGELKGLRLAYFGDGANN
                     MAHSLMLGGVTAGVHVTIAAPAGFEPHSEFVVAAEQRALQTGATVRVTNVPKAAANGA
                     DVLVTDTWTSMGQENDGLDRVGPFRPFQLNADLLSLADPEVVVLHCLPAHRGFEITDE
                     VIDGPQSAVWDEAENRLHAQKALLVWLLERSQ"
     gene            6533..7024
                     /locus_tag="VFC01_13230"
     CDS             6533..7024
                     /locus_tag="VFC01_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HZN80630.1"
                     /translation="MTTATRVGRQARIVSILSSQSVHSQTELAAMLADEGIDVTQATL
                     SRDLEELGAVKLRGADGGVGVYIVPEDGSPVRGVSGGTERMSRLLGELLVSTDASGNL
                     AVLRTPPGAAHYLASAIDRAALPYAVGTVAGDDTILVVAREPMTGAELAAKLESIQQK
                     EIG"
     gene            7025..8227
                     /locus_tag="VFC01_13235"
     CDS             7025..8227
                     /locus_tag="VFC01_13235"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZN80631.1"
                     /translation="MSERVILAYSGGLDTSVAISWIGKETGREVVAVAIDLGQGGEDM
                     EVVRKRALDCGAVEAVVVDAKDEFAEEYCLPAIKSNALYMDRYPLVSALSRPLIAKHL
                     VAAAREHGGGIVAHGCTGKGNDQVRFEVGFASLAPDLEVLAPVRDYAWTREKAIAFAE
                     ENAIPINVTKRSPFSIDQNVWGRAVETGFLEHLWNAPTKDVYDYTEDPTVNWNTPDEV
                     IVGFEKGVPVSIDGKQVTVLQAIEELNERAGSQGVGRLDVVEDRLVGIKSREIYEAPG
                     AMVLITAHTELEHVTLERELGRFKRNTDQKWGELVYDGLWYSPLKSALEAFVAKTQEH
                     VSGEIRMVLHGGHIAVNGRRSSESLYDFNLATYDEGDTFDQSAAKGFVHVHGLSSKIS
                     ARRDLAGQ"
     gene            8224..9636
                     /gene="argH"
                     /locus_tag="VFC01_13240"
     CDS             8224..9636
                     /gene="argH"
                     /locus_tag="VFC01_13240"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZN80632.1"
                     /translation="MSTNEGSLWGGRFADGPSDALAALSKSTHFDWVLAPYDVAASKA
                     HARVLFRAGLLTEEQRDGLLAGLDSLGEDVADGSFGPLVTDEDVHGALERGLIDRVGE
                     DLGGRLRAGRSRNDQVATLFRLWLRDAIRRVADGVLDVVNALATQAAAHPTAIMPGKT
                     HLQSAQPVLLAHHLLAHAHPLLRDVDRIADLDKRAAVSPYGSGALAGSSLGLDPDAIA
                     DELGFDAAADNSIDATAARDFAAEAAFVLSMIGVDLSRLAEDIILWSTTEFGYVTLHD
                     SWSTGSSIMPQKKNPDIAELARGKSGRLIGNLTGLLATLKAQPLAYNRDLQEDKEPVF
                     DSVLQLELLLPAMAGLVGTLRFDVDRMAELAPLGYTLATDVAEWLVRRGVAFRVAHEA
                     AGAAVRAAEARGVGLEDLEDAELAEIHPELTGEVREVLTVDGSVNSRDARGGTAPIQV
                     AKQLGVVRDTADRLRARLRR"
     gene            9826..10215
                     /locus_tag="VFC01_13245"
     CDS             9826..10215
                     /locus_tag="VFC01_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80633.1"
                     /translation="MSIIKNRKKSGTAALGAAAAAAPAVVIPLLLFGPGIAPAYADGM
                     GPTPAPVPSPSRSEILEPPPWLTDDITRNWLADYVRLVGPRSAPPAPSSTDVIGDIIV
                     DVGEAVAPTLPGPSAVVPVLPAGEFPH"
     gene            complement(10248..10859)
                     /locus_tag="VFC01_13250"
     CDS             complement(10248..10859)
                     /locus_tag="VFC01_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005517380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN80634.1"
                     /translation="MSLGPALQFADEHGRIHEHVLDAAERVSIGRSPDATIPLEADST
                     VSRLHAIIESVDSNWTVIDDGLSRNGTFVNGERITGRRLLRSGDKICIGEYVLTFRDA
                     IRPALTMTSAAGGIPTRSSLTDAQLKVLVALCRPYKGGSAYTVPTSNQQIADELYIST
                     ETVKTHLRALFTKFALDENPHPNKRARLVEKAMLSGVVTDRDL"
     gene            complement(10974..12413)
                     /locus_tag="VFC01_13255"
     CDS             complement(10974..12413)
                     /locus_tag="VFC01_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HZN80635.1"
                     /translation="MFRTVGDQVSLWEAVLPAELLKLPDELARVDGLLDDPVFFAPFV
                     AFFDPRIGRRSTPMETYLRLMFLKFRYRLGYESLCREVSDSITWRRFCRIPLDGSVPH
                     PTTLMKLTTRCGSAAVDGLNEALLVKAAEAKLLRTNRLRADTTVVPANVCYPTDSGLL
                     AKAIRRIAATGRRIQAAGGAVGTRVRDRSRAAGKRAHAIAAKLRSRAALGRDEAKAAV
                     LKTTGELAELAETAARDAARLLVNANRAVRRATTKAAQLRARGEHDAAAGRRRGRLVR
                     AVNDLTKLVAATRQIAAQTRQRLVGTTPDGATRRVSLHDPHARPIAKGRLGKPVEFGH
                     KAQVTDNDDGVIVDHSVEMGNPPDAPQLAPAVERVITRTGRTPRTVTADRGYGEKRVD
                     DALHKLGVRNVVIPREGRPGATRRSQEHRPAFRRTIKWRTGCEGRISTLKRGYGWDRA
                     RIDTTEGTRIWVGHGVLAHNLVKISALTA"
BASE COUNT         2042 a         4167 c         4244 g         2064 t
ORIGIN      
        1 ccccgccgag ctggcggccc gacctgcgcg agcccgccga cctcgtggag gaggtgctgc
       61 gactggaggg tctcgagatc atcccgtcgg tgctgccaca ggcgccggcc gggcgtggcc
      121 tcaccgccat ccagaagcgc cgccgtgcga tcggaaagtc gttggcgctc aacggctatg
      181 tcgaaatcct gccaacgccc ttcttgcctg ccggcgtctt cgaccagtgg ggactgcccg
      241 ctgatgatcc gcgccgcaac acgactcacg tgctcaaccc gttggagtcc gaccgcccgg
      301 cgctggcgac gacgctgctg ccggggctgc tggaagcctt ggggcgcaac gtgtcccgcg
      361 gtgcggtcga tgttgcgctg tacgcgattc agcaagtcgt cgagccgact tcggatacgc
      421 gtgccgtcga ccgcatcccg aacaatcgca ggcccaccga cgaggagatc gcggtactcg
      481 acgcgtcctt gcctgtacag ccgcagcacg tcggcgtggt gttggcagga ttgcgtgagc
      541 cggcgggccc atggggtccc gggcgaccgg tggaggcaca tgatgcgttc gaggcggtcc
      601 gcgtcatcgg gcgtgccgcc ggtgtcgagc tgacgctgcg gcccgcacag catctgccgt
      661 ggcatcccgg ccgctgcgcc gaggtgctcg tcggcgatgt ggttgttggc cacgcgggac
      721 agctgcaccc tgcggtggtg gaacggtcgg gcctgccgaa gggcacctgt gcggtcgagc
      781 ttgacctgga cgccatcccg ctgaccgagg cactggccgc gcccgccgtg tcgccgttcc
      841 cggcggtgtt ccaggacgtc agcctgatcg tcggagcgga cgtcgccgcg cagtccgtgg
      901 tcgacgcggt gcgcgaaggc gcaggcgagt tgctcgagga cgtccggctg ttcgacgtct
      961 ataccggccc gcagatcggc gacggccgca agtcggtgac actggccctg cgattccgcg
     1021 ccgccgatcg cacgttgacc gaggacgagg ccagcgcggc gcgcgatgag gcggtcaaga
     1081 gcgccgccga ccgcgtcggc gctattcagc gtgcctagcg cgccgaaact gcggggagat
     1141 cgcagaactg cccgattctc gatctgaccg cagtctggcg agatgtgaga attgatttgc
     1201 aacagtctgc ataaccatgc agaatcgctg catgacgtct gtcgctgtgg ccggtgccag
     1261 cggatatgcc ggtggagaga tcctgcggct gcttctcggc catcccgcct atgccgacgg
     1321 gcggctgacc atcggcgcgc tgacggccgc ggcgagcgta ggtaccacgg tcgccgaaca
     1381 tcatccacac ctgcttccgc tggcaggccg ggtgctcgag gcgaccgaca tcgacgtact
     1441 ggccggccac gacgtcgtgt tcctggcgct gccgcatggg cactccgcgg cgttggccga
     1501 gcagctcgct cccgacaccg tggtcatcga ctgcggggcc gacttccggc tgaccgattc
     1561 ggcggcgtgg gagaagtttt acggctcggc ccacgcgggt agctggccct acgggctgcc
     1621 cgagctgcct ggcgcacgcg accgactgcg cgacgccaag cggatagccg ttcctggctg
     1681 ctatccgacc gcggccctgc tggcactgtg gcccgctgtc gcagaggacc tcatcgagcc
     1741 tgcggtcacg gtcgtcgccg tcagcggcac ctccggagcg ggccgggccg ccaaggccga
     1801 cctgctcggc tcggaggtca tcgggtcggc ccgcgcctac aacatcggcg gaaagcaccg
     1861 gcacaccccg gagatcgcgc agggcctcaa agccctgacc gacaaggatg tcaccgtgtc
     1921 tttcacgccg gtgctgatcc cggcgtcgcg tggcattctc gcgacgtgca ccgctcgcac
     1981 cggcacgccg ctctcgcaac tccgcgccgc ctacgaaaag gcttacgatg cagagccttt
     2041 cgtgcacctg ctgccggagg gccagctgcc gaagaccggc gcggtgatcg gcagcaacgc
     2101 cgctcagttg gcggttgccg tcgatgagga cgcggaaacg ttcattgcaa tcgcggcaat
     2161 cgacaacctt gtcaagggga cgggcggcgc ggccatgcag tcgatgaacc tcgcattggg
     2221 ttggccggag acggaagggc tttcgatcgt gggagtggcg ccatgacgga catcaccgaa
     2281 accacgcggc tcgtgcggac gcagggcgtc accgcgcctg cgggctttcg cgccaccgga
     2341 atcgcggcgg gcatcaaggc ttccagtgcg ctcgacctgg ccctggtgtt caacgaaggc
     2401 cccgattacg ccgccgcggg cgtgttcacc cgcaacaagg tcaaggcggc gccggtgttg
     2461 tggtcgcaac aggtgctgac caccggtcgg ctgcgggccg tgattctgaa ttccggcggc
     2521 gccaacgcct gtactggccc gcaggggttc caggacaccc atgccactgc tgaagcggtc
     2581 gctgcagcgc tgtccgactg gggcaccgaa accggcgcca tcgaggtcgc ggtctgctcg
     2641 accgggctga tcggcgaccg gctgccgatg gataaggtgc tggccggcgt gaccgaggtg
     2701 gtgcacgaga tggccggcgg actgaccggc ggcgaggagg ccgcccgcgc catcatgacc
     2761 accgacaccg tgccgaaaca ggttgcgctg caccacagta acaaatggac cgtcggcggc
     2821 atggccaagg gcgcaggcat gctcgcgccg tcgctggcca ccatgctcac cgtcatcacc
     2881 accgacgctg tcgccgacgc tgcggccctc gaccacgcgc tgcggaaggc gaccgcactc
     2941 accttcgacc gcctcgacgt cgacggtagc tgctcgacca acgacaccgt gctgctgctg
     3001 gcctccggcg ccagcgagat cacacccagc caaacggaat tggacgacgc ggtgcataga
     3061 gtgtgcgacg atttgtgcgc acagttgcag gctgacgccg agggtgtcac caaacgcgtc
     3121 gtcgtcaccg tgaccggtgc agcgtcggag gacgacgcct tgaccgccgc gcgtgtggtg
     3181 gcccgcgaca gcctggtcaa gaccgcgttg ttcggctccg atccgaactg gggccgggta
     3241 ctggcggccg tcggcatggc accggtgacc ctcgacgccg agcggatcag cgtgtcgttc
     3301 aacggttctc cggtgtgcat cgacggcacc ggcgcacccg gcgcccgcga cgtcgacctg
     3361 tccgaccccg agatcgacgt caccatcgat ctcggggtcg gaacgcagtg ggcaagcatc
     3421 cgcaccaccg atctgtccca tggctacgtc gaagagaact cggcgtacag ctcatgaccc
     3481 tcgcgacgcc ggtcaaggcg caggtgctcg cggccgcgct gccatggctc aaacagatgc
     3541 acgacaagat catcgtggtc aagtacggcg gcaatgcgat gaccgacgac gtactgaagt
     3601 ccgccttcgc cgccgatatg gtcttcctgc gtaactgcgg catccatccg gtcgtcgtgc
     3661 acggcggcgg cccacagatc agcagcatgc tcaaaaggct tggcatcgaa ggtgatttca
     3721 agggcggctt ccgggtcacc acgccggaag tgctcgacgt cgcgcggatg gtgctgttcg
     3781 gccaggtggg gcgcgagctg gtgaatctga tcaacgcgca tggcccgtac gccgtcggcg
     3841 tcaccggcga ggatgcccac ctgttcaccg cggtgcggcg cagtgtgacg gtcgacgggg
     3901 tagccaccga catcggtctg gtcggcgatg tcgagaaggt cgacgcctgc gcgctccgcg
     3961 atcttattgg cgcaggccgc atcccggtgg tttcgacgat cgctcccgac accgacggcg
     4021 tcgtgcacaa catcaacgcc gacaccgccg ccgccgcgct ggccgaagcg ctcggtgccg
     4081 agaagctggt cgtgctgacc gacgtcgaag gcctgtacac cagctggccc gaccgggatt
     4141 cgttggtcag cgagatcgac accgccgcac tgacgcaact gcttccgaca ctggagaccg
     4201 gcatggtgcc caagatcgag gcgtgcttgc gcgccgtctc gggcggggtg ccgagcgcac
     4261 acgtcatcga cggccgcgtc gagcactgcg tgctggtcga gttgttcacc gacgaaggga
     4321 cgggcaccaa ggtggtgagc gcatgactct ccaacagcgt tgggaagccg tgatgatgaa
     4381 caactacggc accccgccgc ttgccttggc cagtggtgac ggcgctgtgg tgaccgacgt
     4441 cgacgggaag tcctacatcg acttgctcgg cggcatcgca gtcaacatcc tcgggcaccg
     4501 ccatccggcc gtcatcgacg ccgtcacccg ccagctcaac acgctcgggc acacgtccaa
     4561 cctgtatgcc accgaacccg gtatcgcgct ggctgaatcg ctcgtcggcc tgctcggtgc
     4621 cgatgcacgg gtgttcttct gcaactccgg caccgaggcc aatgaggtgg ccttcaagat
     4681 cacccggctt accggtcgca cgaaacttgt tgccgcccat ggggctttcc atggccgaac
     4741 gatgggctcg ctcgcgctga cgggccagcc gtccaagcag cagccgttcg cgccgctgcc
     4801 cggcgacgtc acccatgttc cgtatggcga cgttgccgca ctcgaagccg ccgtcgacga
     4861 cgcgaccgct gccgtattcc tggagccgat catgggcgag ggcggcgtgg tcgtcccgcc
     4921 cgccggctac ctggtcgcgg cgcgcgagat cacgtccaag cacggcgcgt tgctggtcct
     4981 cgacgaagtg cagaccggaa tgggccgcac cggagccttt ttcgcgcacc agcatgacgg
     5041 gatcacgccc gacatcgtca ccttggccaa agggttgggc ggcggactgc cgatcggcgc
     5101 ctgcctggcg gtcggcacga ccggcgatct gctcaccccc ggcctgcacg gcagcacgtt
     5161 cggcggcaac ccggtgtgca ccgccgcggc gctcgcggta ctcaaggtgc tcgccgacga
     5221 cgatctgatc ggccgcgccg acgtgctcgg caagatgctc agccacggca tcgaatcgct
     5281 gggccatccg ctcgtcgact acgtccgcgg ccgcggactg ctgcaaggcg ttgtgttgac
     5341 gaccgaagtt gccaaacccg ttgagtccgc cgcgcgtgag gccggcttcc tggtgaacgc
     5401 cgccgcgcct gacgtgatcc ggttggcgcc gccgctggtc atcaccgagg gccagatcga
     5461 cgacttcctc aaggcgcttc ccggcgtgct ggacaagggc acccagtcat gacacggcat
     5521 ttcctgcgcg acgacgactt gacaccggac gaacaggccg aggtgctggc gttggccgcc
     5581 gagctgaaga aggacccatt cagccgccgc ccactcgacg ggccgcgcgg tgtcgccgtc
     5641 atcttcgaca agaactccac ccgcacccgg ttctccttcg agatcggcat cgcgcaactc
     5701 ggcggacacg cggtcgtggt cgacggccgc agcacccagc tcggacgtga ggagaccctg
     5761 gaagacaccg gccgggtgct gtcccgctat gtcgacgcga tcgtgtggcg cacgttcgcc
     5821 caggaacgct tgacagccat ggcgtgcgga tccagtgtgc ccatcgtcaa tgcgctgtcc
     5881 gatgagttcc atccgtgcca ggtgctcgcc gatctgcaga ccctggccga acgcaagggg
     5941 gagctgaagg gcttgcggct ggcctacttt ggcgacggtg ccaacaacat ggcgcactcg
     6001 ctgatgctgg gcggcgtcac cgccggcgtg cacgtcacca tcgctgcgcc agcgggtttc
     6061 gagccccact cggaattcgt cgtcgccgcc gagcaacgcg ccctgcagac cggtgccacg
     6121 gtacgggtca ccaacgtccc gaaagcggct gccaacggtg ccgacgtcct ggtcactgac
     6181 acgtggacct cgatggggca ggagaacgac gggctggacc gggtggggcc attccgcccg
     6241 ttccaactca acgccgatct gctgagcctg gccgacccag aagtggttgt gctgcattgc
     6301 ctcccggcac accgcggatt cgagatcacc gacgaggtca tcgacggacc gcagagcgcg
     6361 gtgtgggacg aggccgagaa ccggctgcac gcccaaaagg cgctgctggt ctggctattg
     6421 gaacgatccc aatgacttcc cgaagacgag cgcgactgag gatcgcagcg aggtacgagc
     6481 gaggaccggt gggcccacgc ccgcagggca ggggacgagc gggagtcgag gcatgactac
     6541 agcgacccgg gtagggcgac aggcccgcat cgtttcgatc ctgtcctcgc aatctgtgca
     6601 cagccaaacc gaactggccg cgatgctggc cgacgaaggc attgacgtca cccaggccac
     6661 gctgtcgcgt gacctcgagg aactcggcgc cgtcaaacta cgcggcgcgg acggcggcgt
     6721 cggcgtgtac atcgtgcccg aggacggcag cccggtgcgc ggggtttccg gtggcaccga
     6781 acgcatgtca cgcctgctgg gcgaactgct tgtgtccacc gacgccagcg gcaacctcgc
     6841 cgtgctgcgc accccgcccg gcgcggccca ctacttggcc agcgcgatcg accgcgccgc
     6901 actgccctac gcggtcggca ccgtcgccgg cgacgacacc atcctcgtgg tggcgcgtga
     6961 gccgatgacc ggcgctgaac tggccgcgaa gctggaaagc atccaacaaa aggagattgg
     7021 gtaaatgtcc gagcgcgtca tcctggcgta ttccggtggt ttggacacct cggtggcgat
     7081 cagctggatc ggcaaggaga ccggacgtga agtcgtcgcg gtcgcaatcg atctcggcca
     7141 gggcggcgag gacatggagg tggtccgcaa gcgggcactc gactgcggcg cggtcgaggc
     7201 ggtcgtcgtc gacgccaagg acgagttcgc cgaggagtac tgcctgcccg ccatcaaatc
     7261 caatgcgctg tacatggacc gctacccgct ggtgtcggcg ctgtcccggc cgctgatcgc
     7321 caagcacctg gtggccgcgg cgcgcgagca cggcggcggc atcgtcgcac acggctgtac
     7381 aggaaagggt aacgaccagg ttcgcttcga ggttggcttc gcttcgctgg caccggatct
     7441 cgaggtgctg gctccggtcc gcgactacgc gtggactcgg gagaaggcca tcgcgttcgc
     7501 tgaggagaac gcgatcccga tcaatgtcac gaagcgctcg ccattttcga tcgaccagaa
     7561 cgtgtggggc cgcgccgtgg aaaccggttt cctcgagcat ctgtggaacg caccgaccaa
     7621 ggacgtctac gactacaccg aggacccgac cgtcaactgg aacacacccg acgaggtgat
     7681 cgtcggattc gaaaagggtg taccggtgtc gatcgacggc aagcaggtga ccgtcctgca
     7741 ggccatcgag gaactcaacg agcgtgccgg ttcgcagggc gtcggccggc tcgacgtcgt
     7801 cgaggatcgg ctggtcggta tcaagagccg cgaaatctac gaggcaccag gcgcgatggt
     7861 gctcatcacg gcgcataccg agctcgagca cgtaacgctg gagcgcgaac tcggccggtt
     7921 caagcggaac accgatcaga agtggggcga gctggtgtac gacggcttgt ggtactcgcc
     7981 gctgaagtcc gcgctggagg cgttcgtcgc caaaacccag gagcatgtgt cgggcgagat
     8041 ccggatggtg ctgcatggcg ggcacattgc ggtcaacggc aggcgcagct ccgagtcgct
     8101 gtacgacttc aacctcgcca cctacgacga gggcgacacc tttgaccagt cggcggcgaa
     8161 gggcttcgtg catgtacacg gcctgtcgtc gaagatctcg gcgcgacggg acctcgccgg
     8221 ccagtgagca ccaacgaagg ctcgctgtgg ggcggccggt tcgccgacgg tccgtccgac
     8281 gcccttgccg cgctgagcaa gtcgacgcac ttcgactggg tgttggcgcc gtacgatgtc
     8341 gccgcgtcga aggcgcacgc gcgcgtgctg tttcgggcgg gtctgctgac cgaggagcag
     8401 cgcgacggtc tgctcgccgg tctggacagc ctgggcgaag acgtggccga cggcagcttc
     8461 ggtccgctgg tcaccgacga ggatgtgcac ggcgcgcttg agcgcgggct gatcgatcgc
     8521 gtcggagagg acctcggggg gcggctgcgc gcgggccggt cgcgaaatga ccaggtggcc
     8581 actctatttc ggctgtggct gcgcgacgcg atccgccggg tcgccgatgg tgttctcgac
     8641 gtcgtcaacg cgctggcgac ccaggcggcc gcgcatccga cggcgatcat gccgggcaag
     8701 acgcacctgc agtccgcgca accggttctg ctcgcacatc atctgctcgc acacgcgcat
     8761 ccgctgctgc gcgatgtgga ccgtatcgcc gacctcgaca agcgggcggc ggtctcgccg
     8821 tacggatcgg gggcattggc ggggtcgtcg ctgggccttg atcccgacgc gatcgccgac
     8881 gaactcggct tcgacgcggc ggccgacaac tcgatcgacg cgaccgcggc ccgcgacttc
     8941 gccgcggagg cggcattcgt gctgtcgatg atcggagtcg acctatcacg gctggccgag
     9001 gacataatcc tctggagcac aacggaattc ggatacgtca cgctgcacga ctcgtggtcg
     9061 acgggcagct cgatcatgcc gcagaagaag aatcccgaca tcgcggagct ggcccgcggt
     9121 aagtccggca ggctgatcgg gaacctgacc gggttgctgg caacgctcaa ggcgcagccg
     9181 ctggcctaca accgcgacct gcaagaggac aaggagccgg tgttcgactc ggtgctgcag
     9241 ctagagctgc tgctgccggc gatggcgggc ctcgtaggca cgctgcggtt cgatgtcgac
     9301 cggatggccg agctggcgcc gttgggctac acgttggcca ccgatgtcgc ggaatggctt
     9361 gtgcgcaggg gagtggcgtt ccgggtcgcg cacgaggcgg ccggtgcggc ggtgcgtgcc
     9421 gccgaggccc gcggcgtcgg cttggaggat ctcgaggacg ccgagctcgc cgagatccat
     9481 ccggaactga ccggagaggt gcgcgaggtg ctcacggtcg acgggtcggt caactcgcgc
     9541 gacgcccggg gcggtacggc gcccatccag gtcgccaagc agctcggcgt cgtccgcgac
     9601 acagccgacc ggctccgggc tcggctgcgg cgctagtcgt tctgacgagc aagaccacct
     9661 cttcctgcga gttcgtcgct gctcctccct cgcaggggtg aactcaacca cacccgaagg
     9721 tgatcccaat cccccgcctg agcgatggat cgccatgtgg gttggcccga aactgtgtgt
     9781 acgcgcaacc agcgcaaacc actgaccaga ccgagggaga acctcatgag catcatcaag
     9841 aatcggaaga agtcgggcac cgccgcactc ggtgcggctg ccgcggcggc accggcggtc
     9901 gtgattccgc ttctgctctt cggccccggg atcgccccgg cctacgcaga cggaatgggt
     9961 cccactccag ccccggtacc cagtccatcg cgctccgaga tcctagagcc cccgccgtgg
    10021 ttaacggacg acatcactcg caattggctc gcggattacg tgcgtctcgt ggggccgcgg
    10081 tcggcgccgc ctgcgccttc gtccactgac gtaattggcg acatcatcgt agacgtgggg
    10141 gaagctgtgg cccccaccct tcccggccct tccgccgttg tccccgtcct tcccgcaggc
    10201 gaatttccgc attaaacctg aaccgtcgtc aggacggggc ccgcgtgtca cagatcgcgg
    10261 tcggtgacca cgccgctaag catcgccttt tcgacaaggc gtgccctctt gttgggatgc
    10321 gggttctcgt ccagcgcgaa tttcgtaaac agcgcgcgca aatgcgtttt cacggtttcg
    10381 gtgctgatgt acagctcgtc ggcgatctgc tgattcgacg tggggacggt atacgcagag
    10441 ccgcccttat agggccgaca gagtgcgacg agcaccttga gctgcgcgtc ggtgagcgaa
    10501 ctgcgcgtgg ggattccgcc tgcggcgctc gtcatcgtta acgccgggcg gatggcgtcg
    10561 cgaaacgtca gcacgtattc gccgatgcat attttgtcac cggagcgcaa aagacgccgt
    10621 ccggtgatcc gctcaccgtt gacgaatgtg ccgttacgcg acagcccgtc atcgatgacg
    10681 gtccagttgc tatccaccga ttcgatgatc gcgtgcagtc gcgagacggt gctatcggcc
    10741 tctagcggga tggtggcgtc tggcgatcgc ccgatgctga cccgttcggc tgcatcgagt
    10801 acgtgctcgt ggatgcggcc gtgctcgtcg gcgaactgca gcgccggccc gaggctcatg
    10861 actgcctcac gaccttcagt ttagctactt cgacctgaaa aacccgcgtc ggtgttgagc
    10921 gggggtgtcg ggttgtggcg gttcggtggt gtgggtggcc ctggtgtttt cggtcatgcg
    10981 gtgagtgcgc tgatcttgac gaggttgtgg gctaagacgc cgtggccgac ccagatccgg
    11041 gttccttcgg tggtgtcgat gcgggcgcgg tcccatccgt agccgcgttt gagggtgctg
    11101 atgcggcctt cacatccggt gcgccacttg atggttcgcc ggaatgcggg tcggtgttcc
    11161 tggctgcggc gggtggcgcc gggtcggcct tcgcggggga tgacgacgtt gcggacgcct
    11221 agtttgtgca gggcgtcgtc gacgcgtttc tcgccgtagc cgcggtcggc ggtgacggtg
    11281 cgcggtgttc ggccggtgcg ggtgatgacc cgctcgaccg cgggcgccag ttgcggggca
    11341 tcgggcgggt tgcccatctc gacactgtga tcgacgatga cgccgtcgtc gttgtcagtg
    11401 acctgggctt tgtggccgaa ctcgaccggc ttgcccaagc ggcccttggc gatcgggcgc
    11461 gcgtgcgggt cgtgcaggct gacccgccgg gtggcgccgt cgggagtggt gccgaccagt
    11521 cgctgccggg tctgtgcggc gatctggcgg gtcgcggcca ccagcttggt caggtcgttg
    11581 accgcacgca cgagtcggcc gcgacgccgg ccagctgccg cgtcgtgctc gccgcgagca
    11641 cgcagttggg cggccttggt ggtggcgcgg cgcaccgccc ggttggcgtt gaccagcagg
    11701 cgcgcggcat cgcgtgcggc ggtttcggcc agctcggcca gctcaccggt ggtcttcagc
    11761 accgcggcct tggcctcgtc acggcccagt gcggcgcgag accgcagttt cgccgcgatc
    11821 gcgtgtgccc gctttcccgc cgcgcgcgat ctgtcacgca cccgggttcc gaccgcgccg
    11881 ccggcggcct ggatccgccg acccgtcgcg gcgattcgcc gtatcgcctt ggccagcaac
    11941 ccggaatcgg tcggatagca cacgttggcc gggaccaccg tggtgtccgc acgtaaccgg
    12001 ttggtgcgca acagcttcgc ctcggcggcc ttcaccagca gcgcctcgtt gagcccgtcg
    12061 accgccgcac tgccgcaccg ggtggtcagc ttcatcaacg tcgtcggatg cggcaccgac
    12121 ccatccagcg gaatccggca gaaccgccgc caggtgatcg aatcactgac ctcccgacac
    12181 agcgactcat aacccagccg gtaccgaaac ttcagaaaca tcaaccgcag ataagtctcc
    12241 atcggcgtgg accgacgccc gatccgtgga tcgaagaacg ccacgaacgg ggcgaagaac
    12301 accggatcat cgagcaaccc gtcgacccgc gccaactcgt cgggcaactt caacaactcc
    12361 gcaggaagca ctgcctccca cagcgacacc tgatcgccta cagtacgaaa cacgatggcc
    12421 tcaatccctt cttacccaag ggcttgaggc catccttcct gttcaacacc gtcaacccgc
    12481 ggatcaacgc gccgactttt tcaggtcgaa gtagcta
//
LOCUS       DASRJA010000303         5049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_674116,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000303 DASRJA010000000
VERSION     DASRJA010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674116"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFC01_13260"
     CDS             complement(<1..655)
                     /locus_tag="VFC01_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZN80636.1"
                     /translation="MDADDAEEFLEDLYGVRLRLFRKQAARREGPLLTHSRMSVGPLA
                     IDEIYSAGYVEASPDPLNKVVAVWATGGEVSGECDGLTGEAHAGDVTLLSQPHLPHHV
                     RANNVRLTSVVLDQSVVASAATGLPRGQAPIPVRFTSFQPMDAVAARLWKDTVSYVKH
                     SLFADDAVATPLTVGHASRLMAAVTLSTFPNTEATGPTPHDRTDTKPVLLRRAIEYMD
                     "
     gene            complement(786..1961)
                     /locus_tag="VFC01_13265"
     CDS             complement(786..1961)
                     /locus_tag="VFC01_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HZN80637.1"
                     /translation="MALIRTSPSMSAGDIYALIRERRDITRTEIGQLTGLSRTAVSAR
                     VAALAAQGLVIERDLAPSTGGRPASFVTFNADGGVVLSAAIGRSRTRLAVCNLAGDIL
                     AASDIDQEPGLGPDDLMPDIVKRLDALLDDSGLHDEKIYGVGVNLPGTVDRGRGCSLD
                     SPIMSGWDGVPLPPYFAELTSAPVILDNDANVIALAERRGDRQAFDDLLVLKASTGLG
                     AGIIAGGSLQRGAVQAAGEFGHNKTPAAAGLPCRCGDTGCLEAIAGGWALVRALQADG
                     RAVGHLRDVVELANSGDPDARRMIRDSGRHVGEVLAHAVNLLNPAVLVVAGDMAGAYN
                     IFVAGLRETLYGNATALATRVLQVAPSTYGDRSGVVGSATMVLDHVLSPKAIDSSLS"
     gene            2049..3590
                     /gene="zwf"
                     /locus_tag="VFC01_13270"
     CDS             2049..3590
                     /gene="zwf"
                     /locus_tag="VFC01_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003938708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZN80638.1"
                     /translation="MVDPCDFVIFGGTGDLAVRKLLPALYLRDRDGELTADTRIIAVA
                     RAGLDEPGYRHKIQAELGRFVATEFLDEDSVGRFLARLRFVSIDLAEPGDYMAVTDVL
                     KGSNVDIRVFYLACAPALFGPICDALGTQGLVTESSRVVLEKPIGHDLPSAQAINDAV
                     GAVFAERQIFRIDHYLGKESVQNLLVTRFANTLLEPLWNSSWIDHVQITAAESLGVGT
                     RSDYYDKSGALRDMLQNHLLQVLCLVAMEPPTYVDRETVRDEKLKVLQALKPLSAHDI
                     KRDTVTGQYGPGLSERAMVEGYRHDAENPDSSTETFVAVKAEINNWRWAGVPFYLRTG
                     KRMSRRYSEIVVQFKPVPLPMFPGSEGASEPNRLVISLQPDEEMRLELTAKEPGPGGI
                     RMRPVSLALNYTQAFQLRSPDAYERLLMDVVRGDPTLFMRRDEVEAAWAWAEPILQQW
                     QESGRVPRLYPAGTDGPVDAATLLERDGRRWHEGAACRIPSLPMSPRGSPSAAPSNAA
                     HIWPG"
     gene            3506..>5049
                     /gene="edd"
                     /locus_tag="VFC01_13275"
     CDS             3506..>5049
                     /gene="edd"
                     /locus_tag="VFC01_13275"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003938707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HZN80639.1"
                     /translation="MSHSIVADVTARIAERSAKQRGAYLARLATAAENGPARGRLACA
                     NLAHGFAASDTRDKTALRGMVKPNIAIVSSYNDMLSAHKPFEGYPAQLKQAVLEAGGI
                     AQFAGGVPAMCDGITQGRDGMQLSLFSRDVIAMATAIALSHDMFDGALLLGVCDKIVP
                     GMLIGALGFGHLPVIFVPAGPMTSGLPNSEKAHIRQLHAEGKVGRDALLDAEAASYHG
                     SGTCTFYGTANSNQLLMEAMGLHLPGAALVNPDTPLRDALTAEAGRRVTGLTALGDHY
                     TPVGEIVDERAVVNGCVALLATGGSTNHTMHLVAIARAAGITLSWKDIADLSAVVPLL
                     ARVYPNGKADVNHFHAAGGIACIINSLLDAGLMHDDVRTVAGHGLRRYTSEPRLDGDA
                     LQWVDGTRESLDATVLRTADDPFAPDGGLRTLTGNLGTSVIKTSAVDPQHRSITAPAA
                     VFDSQEQFLEAFQSGVMDRDFIAVLRYQGPQSLGMPELHKLTPALGVLQDRGHRVALV
                     TDGRMSG"
BASE COUNT          880 a         1690 c         1639 g          840 t
ORIGIN      
        1 catccatgta ctcgatggcg cggcgcagca gcaccggctt ggtatcggtg cggtcatggg
       61 gtgtcgggcc ggtggcctcg gtgttgggaa acgtcgacag cgtcaccgcg gccattaacc
      121 ggctggcgtg accgaccgtc agcggtgtgg cgaccgcgtc gtcagcgaac aggctgtgtt
      181 tgacgtagct gacggtgtcc ttccacagcc gtgctgcgac cgcgtccatc ggctgaaaac
      241 tggtgaagcg gacgggaata ggcgcttgac ccctcggcag gccggtggcc gcgcttgcga
      301 ccacggattg gtcgagcacc accgaggtca gacgaacgtt gttggcgcgc acgtgatggg
      361 gcaggtgtgg ttgtgataga agggtgacat cgcccgcgtg ggcttcgccg gtcagcccat
      421 cgcactcacc ggatacctcg ccgccggtcg cccagactgc caccaccttg ttcagtggat
      481 cgggcgacgc ctccacgtag cccgccgagt agatttcgtc gatcgcgagc ggtccgacgg
      541 acatacgcga gtgcgtcaac aaaggaccct cgcgacgggc ggcttgtttg cggaacagcc
      601 gcaaccgcac gccgtacaag tcctcgagga actcctcggc gtcatcggca tccatgatct
      661 cgaccgcgta acgttcgggc cgatggttcg gcccagcggt ctcggctcta gcgatcacct
      721 cttacctacc ggataaacgt gggctcagcc gggggctatg aataaggtac ctcgcgagcg
      781 gcccctcagg acagcgacga gtcgatcgcc ttggggctca gcacatggtc gagcaccatc
      841 gtcgcgctac cgacgacgcc cgagcggtcg ccgtaggtcg acggcgccac ttgcaacacc
      901 cgggtggcca atgcggtcgc gttaccgtac aacgtctctc gaaggccagc gacgaaaatg
      961 ttgtaggcgc ccgccatatc gccagccacc accagcacgg cgggattgag cagattcacc
     1021 gcgtgtgcaa ggacttcgcc gacgtgccgg ccgctgtcgc ggatcattcg gcgggcgtcg
     1081 gggtcgccgc tgttggccaa ttccaccacg tcgcgaaggt ggccgacggc gcggccgtcg
     1141 gcttgtaggg cgcggacaag ggcccagccg ccggcgatcg cctcgaggca accggtgtcg
     1201 ccgcagcgac acggcaatcc cgcggcggca ggcgtcttgt tgtggccgaa ctcccccgcc
     1261 gcctgcaccg cgccgcgctg caaagatccg ccggcgatga tgcccgcgcc gaggcccgtc
     1321 gaggccttca gcacgagcag atcgtcgaac gcctgtcgat cgccacgacg ttcggcaagc
     1381 gcgatcacgt tggcatcgtt gtcgagaatc actggggcgc tggttaattc agcgaagtac
     1441 ggcggcaacg gcacaccgtc ccagccgctc atgatcggcg agtcgaggct gcatccgcgg
     1501 ccccggtcga cggtgccagg caggttcacc ccgacgccgt agatcttctc gtcgtgaaga
     1561 ccggagtcgt ccagcagcgc gtcgagccgc ttgacgatgt cgggcatcaa atcgtcggga
     1621 cccagcccgg gctcctggtc gatgtcgctg gccgccaaaa tgtcgccggc caggttgcac
     1681 acggcgagcc gggtgcggct gcggccaatg gccgccgaca agacgacgcc gccgtcggca
     1741 ttgaaggtca caaaactggc cgggcgtccg cccgtcgacg gggctaggtc acgctcaatg
     1801 accaatccct gcgcagcgag cgcagcgacg cgggccgaca ccgctgtccg agacaacccg
     1861 gtcagctggc cgatctccgt gcgggtgatg tcccgccgct ctctgatcaa ggcatagatg
     1921 tcgccggcgg acatcgacgg tgaagtccgg attaacgcca tgttcgtaag cgtaactccc
     1981 gtcacttttg tttttcaaat acctaagtgg gtttgcatgg cgtctgaagt ctctctacgg
     2041 tgggccacat ggttgatcca tgcgacttcg tcatcttcgg cggcaccggc gacctcgccg
     2101 tccgtaagct gctgcccgcg ctgtatctgc gcgaccgcga cggcgagctg accgccgaca
     2161 ccagaatcat cgccgtggcc agggccggcc tcgacgagcc cggctaccgc cacaagatcc
     2221 aagctgaact gggccgattc gtggccaccg agttcctcga cgaggactcc gtcggccgct
     2281 ttctggcccg cctgcggttc gtcagcatcg acctggccga gcctggcgac tatatggccg
     2341 tcaccgacgt gctgaagggc tcgaacgtcg acatccgggt gttctacctt gcctgcgcgc
     2401 ccgccctgtt cgggccgata tgcgatgcgc tgggcacgca gggcctggtc accgagtcgt
     2461 cgcgggtggt gctggagaaa ccgatcggcc acgacctgcc gtcagcccag gccatcaacg
     2521 acgccgtggg cgcggtgttc gcggagcggc agatcttccg catcgaccac tacctcggca
     2581 aggagagcgt gcagaacctg ctcgtcacca ggttcgcgaa caccctgctg gaaccgctgt
     2641 ggaactcgag ctggatcgac cacgttcaga tcaccgccgc cgagtcgctc ggggtcggca
     2701 cgcgcagtga ctactacgac aagtccggcg cgctgcgcga catgctgcag aaccatctgc
     2761 tgcaggtgct ctgtctggtc gcgatggaac cgcccaccta cgtcgaccgt gagaccgtgc
     2821 gcgacgagaa gctcaaggtg ctgcaagcgc tgaagccact gtcggcccac gacatcaaac
     2881 gcgacaccgt gacaggacag tacgggcctg gcctatccga acgcgccatg gtcgagggct
     2941 accggcacga cgccgaaaac ccggacagct caacggaaac gttcgtcgcc gtcaaggccg
     3001 agatcaacaa ctggcgctgg gccggtgtgc ccttctatct gcgtaccggg aagcggatgt
     3061 cgcgccgcta ctccgagatc gtcgtgcagt tcaaacccgt accgctgccg atgtttccgg
     3121 gcagcgaggg cgcctcggag ccgaaccggc tggtgatctc gctgcagccc gacgaggaga
     3181 tgcgcctcga actgaccgca aaagagcctg gacccggcgg aatccggatg cggccggtat
     3241 cgcttgcgct caactacacg caagccttcc agcttcgttc tcccgacgcc tacgagcggc
     3301 tgctgatgga tgtcgtccgc ggcgatccga cgttgttcat gcgccgcgac gaggtcgagg
     3361 cggcctgggc gtgggccgaa ccgattctgc agcaatggca ggagtcgggc cgagtgcccc
     3421 ggctgtatcc ggccggcaca gacggacccg tcgacgccgc caccttgctc gagcgcgacg
     3481 gccggcgctg gcacgaagga gcagcatgtc gcattccatc gttgccgatg tcaccgcgag
     3541 gatcgccgag cgcagcgcca agcaacgcgg cgcatatttg gcccggctag ccaccgcggc
     3601 cgaaaacggc cccgcccgtg gccgactcgc atgtgcgaac cttgcccacg gtttcgcggc
     3661 gtccgatact cgcgacaaga ccgcgctgcg cggcatggtc aaacccaaca tcgcgatcgt
     3721 gtcgtcgtac aacgacatgc tctcggctca taagcccttc gaaggctatc ccgcgcagct
     3781 caagcaggcc gtgctcgagg caggtggcat cgcccagttc gccggcggcg tgccggccat
     3841 gtgcgacggc atcacccagg gtcgcgacgg catgcagctg tcgctgttct cacgcgacgt
     3901 gatcgcgatg gcgacggcga tcgccctatc ccacgacatg ttcgacggtg cgctgctgct
     3961 cggtgtgtgc gacaagatcg tgcccggaat gctgatcggc gcattgggat tcgggcatct
     4021 gccggttatc ttcgttcctg cgggcccgat gacttcgggt ttgcctaata gcgagaaggc
     4081 gcacatccgg cagctccacg ccgagggcaa ggtcggccgc gacgcgctgc tcgacgccga
     4141 ggctgcctct taccacggca gcggcacgtg cacgttctac ggcaccgcga actccaacca
     4201 actgctgatg gaagcgatgg gcctgcacct gccgggtgcc gcgttggtca accccgacac
     4261 cccgctgcgg gatgcgttga cggccgaagc gggccgtcgc gtgacgggcc tgaccgcatt
     4321 gggtgaccat tacacgccgg tcggggagat cgtcgacgaa cgagccgttg tcaatgggtg
     4381 cgttgcgctg ttggccaccg gcgggtcgac caaccacacc atgcacctgg tggccattgc
     4441 ccgcgcggcc ggaatcacgt tgtcctggaa agacatcgcc gatctgtcgg ccgtcgtgcc
     4501 gctgctggcc cgggtgtatc ccaacggcaa agccgacgtc aaccacttcc acgccgcggg
     4561 tggcatcgcg tgcatcatca actcgttgct ggatgcgggt ctgatgcacg acgacgtccg
     4621 cacggtggca ggccacgggc ttcgtcgcta cacatcagaa ccgcggctcg acggtgacgc
     4681 actgcagtgg gtcgacggca ccagggagag cctggacgcc acggtgctgc ggaccgcgga
     4741 tgacccgttc gcccccgacg gtgggctgcg aaccctgacg ggcaatctcg gcaccagcgt
     4801 gatcaagaca tctgccgtcg atccgcaaca ccggtcaatc accgccccgg ccgcagtgtt
     4861 cgacagccag gagcagttcc tcgaggcatt ccaatccggg gtaatggatc gcgatttcat
     4921 cgcggtgctg cgttaccagg gaccgcagag cctcggcatg cccgagctgc acaagctgac
     4981 acccgcactc ggcgtgctgc aggaccgcgg acaccgcgtc gcgttggtca ctgatggccg
     5041 aatgtccgg
//
LOCUS       DASRJA010000304         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2378400,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000304 DASRJA010000000
VERSION     DASRJA010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2378400"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFC01_13280"
     CDS             complement(<1..536)
                     /locus_tag="VFC01_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HZN80640.1"
                     /translation="MTIAAPVRETVNPYLNGNYAPVREEITAIDLDVTGKIPDYLDGR
                     YLRIGPNPMGDPDPAKYHWFLGTGMAHGLRLRDGKAHWYRNRYVRSAAVARALGESWA
                     GGAHTGGVDFAANTNIIGHAGKTFAIAESGVRPYELTDELETVGPSDFCGTLFGGYTA
                     HPKRDPATGELHAVSYNP"
     gene            complement(621..2201)
                     /locus_tag="VFC01_13285"
     CDS             complement(621..2201)
                     /locus_tag="VFC01_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN80641.1"
                     /translation="MAQQTSGLGLGQLLLALDRTMVTLVEAPRGLDMPVGSVALVDAD
                     DVRLGLAVGPGSADLFFLLGVSDAEAVRWIGQQAGRVPAAIFVKEPSPAAVKRAVALG
                     IAIVAVEPQARWELLYRLVNHAFEHHGDRGDPQYDSGTDLFGLAQSIADRTRGMISIE
                     DADSHVLAYSASSDEADELRRLTILGRAGPPEHLEWIGQWGIFDALRASGDVVRVAER
                     PELGLRPRLAVGIHLPPTDSRRRPGFAGTIWLQQGSAPLADDVEEILRGGAVLAARIM
                     SRLAATPSTHTVRVQELLGLIGEGPDVGAIARELGITVDGRAALIGFAGIENSVPADV
                     IALSASAFRADAQIASTGERVYVLLPKTGGSSSVTSWVRGVVAALRRELGLTLRAVIA
                     TPLAGLAGAAGARAEVDRVFDSADRHPGAIGQVSSLDEARTTVLLDEIVSQVAGQPRL
                     VDPRVRQLRDDEPMLADTLQAYLDGFGDIATVAKQLHVHPNTVRYRVRRIEKLLSTSL
                     DDPDDRLVLALGLRATER"
     gene            2295..>2609
                     /locus_tag="VFC01_13290"
     CDS             2295..>2609
                     /locus_tag="VFC01_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003900286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN80642.1"
                     /translation="MDAITDVPFPANEPIHEYAPGSGERTRLTEQLRTLTQDPIDLPH
                     VIGGTHRMGGGQRVDVVQPHRHSARLGTFTNAEHADASAAIEAAMAAKAAWEATPFDE
                     RAA"
BASE COUNT          421 a          911 c          870 g          407 t
ORIGIN      
        1 atggggttat acgacacggc gtgtagttca ccggtggcgg ggtcacgttt cggatgcgcg
       61 gtatacccgc cgaacagcgt gccgcagaag tcggacggcc cgacggtttc cagctcgtcg
      121 gtgagttcgt acggtcgcac cccggactcg gcgatggcga acgtcttgcc cgcatggccg
      181 atgatgttgg tgttggcggc gaagtcgacg ccgccggtgt gcgcaccgcc ggcccagctt
      241 tcgcccaagg cgcgggcgac cgcggcggat cgcacatatc tattgcggta ccagtgcgcc
      301 ttgccatcgc gcagacggag gccgtgtgcc atcccggtgc ccaggaacca gtgatacttg
      361 gccgggtccg ggtcgcccat cgggttcggc ccgatgcgca gatagcggcc gtcgaggtag
      421 tcgggaatct tccccgtgac gtcaaggtcg atcgcagtga tctcctcccg caccggcgcg
      481 tagttgccgt tcaggtacgg gttcaccgtc tcgcggacgg gggctgcaat ggtcatgcct
      541 tgatgctgcg cctataacca ctcaccgcgc gaccccctga cggttgaatt cggtgtccat
      601 cgactggttg acggcgccga tcagcgctcg gtggcccgca atcccaaggc caggaccagg
      661 cggtcgtcgg ggtcgtccag cgacgtcgac aacagctttt cgatccggcg cacgcgatag
      721 cgcacggtgt tcggatgaac atgcagttgc ttcgcgaccg tggcgatgtc accgaatccg
      781 tcgaggtagg cctgaagggt gtccgccagc atcggctcgt cgtcgcgtaa ttgccgcacc
      841 cgcggatcga ccagccgcgg ttgcccggcc acctgcgaca cgatctcgtc gagcagcacg
      901 gtggtgcggg cttcgtcgag tgagctgacc tgtccgatgg cgccgggatg gcgatcggcg
      961 ctgtcgaaca cccggtccac ctcggcgcgc gccccggctg cgcctgccag cccggccagc
     1021 ggggttgcga tcaccgcgcg cagcgtgagg ccgagttcgc gccgcaatgc cgcgacgacg
     1081 ccgcgcaccc acgacgtcac cgacgacgac ccgccagtct tgggcagcag cacatacact
     1141 cgctcacccg tcgacgcgat ttgggcgtcg gcacgaaatg cgcttgcgct caaggcaatc
     1201 acatcggccg gcacggaatt ctcaatgccg gcgaagccga tcaacgcggc gcgcccgtcg
     1261 acggtgatgc ccagctcgcg ggcgatcgcg ccgacgtcgg ggccctcacc gatcagaccg
     1321 agcagttcct ggacgcgcac cgtgtgcgtc gacggcgtcg ccgccagccg cgacatgatg
     1381 cgcgccgcca gcacggcgcc accgcgcagg atctcctcga catcgtcggc cagcggcgcg
     1441 gatccctgtt gcagccaaat ggttccggcg aagccgggcc tgcggcggga atcggtcggc
     1501 ggcagatgga tgccgaccgc gaggcggggc cgcagcccca actccgggcg ttcggcgacg
     1561 cggaccacgt cgccgctcgc gcgcaacgcg tcgaagatgc cccactggcc gatccactcg
     1621 agatgttcgg gcggcccggc gcgcccgagg atggtcaggc gacgcagctc gtcggcctcg
     1681 tcgctggagg ccgagtacgc cagcacatgg gagtccgcat cttcgatgct gatcatcccg
     1741 cgggtgcggt ccgcgatcga ttgcgcgagc ccgaacaggt cggtgccgga atcgtattgc
     1801 ggatcgcccc ggtcgccatg gtgctcgaag gcgtggttga ccagtctgta gagcagctcc
     1861 caccgagcct gcggttcgac ggccacgatc gcgataccca gcgcgaccgc ccgtttgacc
     1921 gcggcgggcg acggctcctt gacgaagatg gccgcgggga cccgtccggc ctgctgtccg
     1981 atccagcgca ccgcctccgc gtccgacaca ccgagcagga agaagagatc cgctgagccg
     2041 gggcctaccg cgagcccgag tcggacgtca tcggcgtcga cgagcgcgac tgaacccacc
     2101 ggcatgtcca ggccgcgggg cgcctcgacc agcgtcacca tcgtgcgatc gagggcgagc
     2161 aggagttgtc caaggccgag cccggatgtc tgctgcgcca taccacactc ctttgtccga
     2221 ttcgactata gatgtcagat ggtgttgtcg gatcagccat gtgttccgcg tcgcaattcg
     2281 gggattcttg ctgcatggac gccatcaccg acgtgccctt cccggccaac gagccgatcc
     2341 acgaatacgc acctggttcg ggtgaacgca cccggctcac cgaacagctt cggacgctca
     2401 cccaagaccc catcgacctg ccgcatgtca tcggcggcac ccaccgcatg ggcggcggcc
     2461 aacgcgtcga cgtggtccag ccgcaccggc actccgcgcg gctgggcacc ttcaccaacg
     2521 ccgagcacgc agacgcctcc gcggcgatcg aagcggcgat ggccgccaag gccgcctggg
     2581 aggccacccc gttcgatgag cgcgccgcc
//
LOCUS       DASRJA010000305         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1834450,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000305 DASRJA010000000
VERSION     DASRJA010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834450"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /gene="aroC"
                     /locus_tag="VFC01_13295"
     CDS             <1..1144
                     /gene="aroC"
                     /locus_tag="VFC01_13295"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZN80643.1"
                     /translation="VAGVRVTSDDIAGQLARRRLGYGRGARMKFEQDQVTVLAGVRHG
                     VTLGGPIAIEVGNTEWPKWETVMAPDPVDPAELDVARNAPLTRPRPGHADYAGMLKYG
                     FDDARPVLERASARETAARVAAGTVARAFLKEALGVEVVSHVISIGTSKPYDGPPPQA
                     SDLAAVDASPVRAFDEESEKLMIAEIEAAKKDGDTLGGIVEVVAHGLPVGLGSFTSGD
                     NRLDSQLAGAVMGIQAIKGVEIGDGFETARRRGSVAHDEMYPGPDGVMRSTNRAGGLE
                     GGMTNGQPLRVRAAMKPISTVPRALATIDMATGDEAVAIHQRSDVCAVPAAGVVVETM
                     VALVLARATLEKFGGDSLAETRANIDTYLRSVADREPATQPTQASG"
     gene            1144..1644
                     /locus_tag="VFC01_13300"
                     /pseudo
     CDS             1144..1644
                     /locus_tag="VFC01_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
     gene            1784..>2269
                     /locus_tag="VFC01_13305"
     CDS             1784..>2269
                     /locus_tag="VFC01_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZN80644.1"
                     /translation="MTEPVTVDVLVDPPYPVIIGTGLLGELVRILDGRHKVAILHQPA
                     LAQTAEAIRNHLSDKGIDAHRIEIPDAEAGKELPVVGFIWEVLGRIGVGRKDAIISLG
                     GGAATDVAGFAAATWLRGVDIVHVPTTLLGMVDAAVGGLVIETSCQPAEFGREHQANR
                     HR"
BASE COUNT          380 a          793 c          775 g          321 t
ORIGIN      
        1 ggtcgccggc gtccgcgtca cctctgacga catagcgggc cagctggccc gccgccgtct
       61 cggctacggc cgcggcgccc ggatgaagtt cgagcaggac caggtcaccg tgctggccgg
      121 cgtccgtcat ggggtcactc tcggcggccc gatcgcgatc gaggtcggca acacggagtg
      181 gcccaagtgg gaaaccgtga tggcccccga cccggtcgac cccgcagaac tcgacgtcgc
      241 ccgcaacgca ccgctgacca ggccacggcc cggccacgcc gactacgcag gcatgctcaa
      301 gtacggcttc gacgacgccc gcccggtgct cgagcgcgcc agcgcccgcg aaaccgccgc
      361 gcgcgtcgcg gccggaaccg tcgccagggc gtttctgaag gaggcgctcg gcgtcgaggt
      421 ggtgtcgcac gtcatctcca tcggcacgtc caagccctac gacggcccgc cgccgcaggc
      481 ctcggacttg gcggccgtcg atgcgagccc ggttcgggcc ttcgatgaag agtccgagaa
      541 gctgatgatc gccgagatcg aggccgccaa gaaggacggt gacacgctcg gcggcatcgt
      601 tgaggtggtc gcccacgggc tgcccgtcgg gctcggctca ttcaccagtg gcgacaatcg
      661 gctcgacagt caactggccg gggcggtgat gggcattcag gcgatcaagg gcgtcgagat
      721 cggcgacggt ttcgaaaccg cgcgccggcg tggcagcgtc gcccacgacg agatgtaccc
      781 gggccccgac ggtgtgatgc gttcgacgaa tcgggccggc ggtctcgagg gcggcatgac
      841 caacggccag ccgttgcgcg tacgcgccgc gatgaagccg atctcgaccg tgccgcgcgc
      901 gctggccacc atcgacatgg ccaccggcga cgaggccgtc gcgatccatc agcgctccga
      961 tgtgtgcgct gtgcctgccg cgggcgtggt ggtcgagacg atggtcgcgc tggtgctggc
     1021 ccgcgccacc ctggagaagt tcggcggcga ctcgctcgcc gaaacgcggg ccaacatcga
     1081 cacgtacctg cgctcggtgg cggaccgcga gccggccacg caaccgacgc aggcgtcggg
     1141 ctgatggcgc ccaaggcggt actgatgggc atgccaggat ctggcaagtc caccatcgga
     1201 cgtcggctgg ccaaggcgct ggacctgacg ctgctcgaca ccgacgccaa gatcgtcgag
     1261 acaacgggcc gcaccatcgc cgacatcttc agcaacgacg gcgagcccga attccgccgc
     1321 gtcgaggaac aagtggtgcg cgccgcgctt gccgagcacg acggcattat ttcgcttggc
     1381 ggcggcgcga tcacctcacc gggcgtccgc gacgcgctgg ccggtcacac tgtgatcttt
     1441 ctcgaaatca gcgcggccga agggattcgg cgcaccaccg gcggcaccgt ccgtccgttg
     1501 ctggcgggcc ctgaccccgc ccagaagtac cgcgatctga tgtccgaacg ggttccgctg
     1561 taccggcggg ccgccacgat gcgcgtcaac accaaccgcc gcaatccggg tgcggtggtg
     1621 cgccacatcg tcgagcggct ggaaaatccc gacgcgccac gatcgcagcg ccggcggcgc
     1681 agaccgccgt ggcgccgtgg accgtcgtca ctgacgccgg agccgatcac tgaagcccca
     1741 ccgacacctg cggctgtcgc cgccagaaac acgaaaggac accgtgactg agccggtgac
     1801 agtcgacgtt ctggtcgacc caccttatcc ggtcatcatc ggcaccggtc tgctgggcga
     1861 attggttcgc atcctggatg gcaggcacaa ggtcgcgatc ctgcatcagc cggcgctggc
     1921 gcagaccgcc gaagcgatcc ggaaccacct gtcggacaag ggaatagacg cgcaccgcat
     1981 cgagattcca gacgccgaag ccggcaagga acttccggtt gtcggcttca tctgggaagt
     2041 gttgggccgc atcggtgttg gccgcaagga cgcaatcatc agcctcggcg ggggagcggc
     2101 caccgacgtc gcgggttttg ccgccgccac gtggctgcgt ggcgtggaca tcgtgcacgt
     2161 gcccaccacg ctgctcggca tggtcgacgc ggcggtcggc ggtctcgtca tcgagacgtc
     2221 ctgccagcct gccgagttcg gccgcgaaca ccaggccaac cgacaccgc
//
LOCUS       DASRJA010000306         4493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3683478,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000306 DASRJA010000000
VERSION     DASRJA010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3683478"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..100
                     /locus_tag="VFC01_13310"
     CDS             <1..100
                     /locus_tag="VFC01_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80645.1"
                     /translation="AITVEEGGRVGDITEEAVDASIALLAPRVEPD"
     gene            complement(97..285)
                     /locus_tag="VFC01_13315"
     CDS             complement(97..285)
                     /locus_tag="VFC01_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN80646.1"
                     /translation="MRVEWDESRCALSALCTGLAPDVFRVDDDARLAISSDIDDEQRQ
                     AVQDAADSCPTQAITVAD"
     gene            complement(285..1772)
                     /locus_tag="VFC01_13320"
     CDS             complement(285..1772)
                     /locus_tag="VFC01_13320"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN80647.1"
                     /translation="MSRPAETVDVLIVGAGLSGVGAACQLRQECPDKSVVVLEARDAI
                     GGTWDLFRYPGIRSDSDMFTLGYRFSPWTDPKAIADGPSILRYIHDTAEQYDVDGLIR
                     LNHRVVNANWDSDEARWTVDVHRTDTDELLTISCSFLYVCTGYYRYDEGFSPKFPGVE
                     RFRGPVIHPQHWPEDLDYRGKRVVVIGSGATAVTLVPSLAEQAAHVTMLQRSPTYVAS
                     RPASDAIADRLRAWLPQKLAYAIVRWKNALQAIAIFNLSRRRPEMMKSMLRKAAIKQL
                     PEGYAVDTHFAPSYNPWDQRMCLVPDGDLFTAIRDGRASVVTDHIDTFTETGIRLQSG
                     AELEADIIVSATGLNLLAIGGMQLKVDEQPVNLSKTVSYKGMMLSGVPNFAWTIGYTN
                     ASWTLKADLVAEYVCRLLKHMDANDYATVTPDAAGATAANPFLDLASGYVKRSLAELP
                     KQGDRAPWRLHQNYVKDVRLLRRGPIDDGVVFTPAVADQPARSIA"
     gene            1930..3285
                     /locus_tag="VFC01_13325"
     CDS             1930..3285
                     /locus_tag="VFC01_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN80648.1"
                     /translation="MAVATTDPVRLPPAPRIPKLLQGVGFVAAREKSVAALAKRYGSA
                     FTLRLPIFGETVVVSDPGLIKDLFTTNSDLVGRAGVLGEMFGPGSTFSLDGTEHRERR
                     KLLVPPFHGKRMASYEGIIEEEVLRETASWPEGREFETMPSMMRITLNAILRTVFGAE
                     GAALDELREVLPSLVLHASRLAVLPPLVRRDFGAWSPGGKLTANRTRYDEIIARLISD
                     IRNDPKFDERNDVLSLMLQARYEDGSQISDDHIADELLTLLAAGHETTATTLAWSVER
                     LRRHPRLLARLRAEADAGGSELVQATIWEVQRTRPVINGTARLTKTRIRLGEWVIPEH
                     HAILASISLAHASEDSFGDAKAFNPDRFVGNPPDNYAWIPYGGGVRRCIGAAFANVEM
                     NVTLRTLLREFEFGTTYAAGERLHSRGVATAPAHGGRAVVYRRTTRSLEPTGQTQRVS
                     A"
     gene            3282..4190
                     /locus_tag="VFC01_13330"
     CDS             3282..4190
                     /locus_tag="VFC01_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN80649.1"
                     /translation="MIAREEVLVDVGRGIELCYDQTGDPADPPILLIAGLGQQLHSWP
                     NDFVAALAGRGYRVTRFDNRDAGRSTHMNYPPPNPVAMFRGRNRYHLGDMARDTAGLL
                     DALGYADAHLVGISMGGMIAQTVAAHYPGRVRTLTSIMSNTGAPRLGRPALSTWRRML
                     TSRPPRTRAEAMDRAANMFSHIGSHGFPFDEQAVREYAGIAWDRDASPAGIARQLAGI
                     FASGDRTAELRNIDAPTLIIHGDRDRMVHPTGGSATARAIPGARLETIVGLGHDLPIG
                     AWGRIIDLITDHTTFAPVTHSEEEHA"
     gene            4264..>4493
                     /locus_tag="VFC01_13335"
     CDS             4264..>4493
                     /locus_tag="VFC01_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80650.1"
                     /translation="MSKAQRRTISGRTVVITGAASGIGRALAQRLSAHSCPVAIADID
                     ENGLKETEATLLGPTLLRVLDVADAEAQRDFAA"
BASE COUNT          760 a         1465 c         1516 g          752 t
ORIGIN      
        1 cgcgatcacc gtcgaggagg gcggccgggt gggcgacatc accgaggaag cggtggatgc
       61 gtcgatcgcg ctgctggctc cgcgcgttga gcccgattag tcggcgacgg tgatcgcctg
      121 cgtcgggcac gagtcggcgg cgtcctgaac cgcctgccgc tgctcatcgt cgatgtcgct
      181 gctgatggcc aaccgggcgt catcgtcgac gcggaagaca tcgggtgcca agccggtgca
      241 caaagccgat agggcgcaac ggctttcgtc ccactcgact cgcatcaggc gatgctcctt
      301 gccggttggt ctgcgacggc cggcgtgaac acgacgccat cgtcgatcgg gcctcggcgc
      361 agcaggcgga catccttgac gtagttctga tgcagccgcc acggcgctcg gtcgccttgc
      421 ttgggcagct ctgcgaggct gcgtttgacg tatcccgatg ccaagtccag gaacggattc
      481 gccgctgtgg cacctgccgc gtcgggcgtc acggtcgcat aatcgttggc gtccatgtgc
      541 ttgagcagcc ggcacacata ctcggccacc aggtcggcct tcagcgtcca cgacgcgttg
      601 gtgtagccga tcgtccatgc gaagtttggt acacccgaga gcatcatgcc cttgtaggac
      661 accgtctttg acaggttgac cggttgctcg tcgaccttga gttgcatgcc gccgatcgca
      721 agcaggttca ggccggtggc gctgacgatg atgtcggcct cgagctcggc gccggattgc
      781 agccggattc cggtctcggt gaaggtgtcg atgtggtcgg tgacgacgga tgcccgcccg
      841 tcgcggatgg cagtgaacag atcgccgtcg ggcaccaggc acatccgctg atcccacggg
      901 ttgtatgacg gggcgaaatg ggtgtcgacg gcgtagccct cgggcagttg cttgatcgca
      961 gccttgcgca gcatcgactt catcatctcg ggacggcggc ggctcaggtt gaagatcgcg
     1021 atcgcttgta atgcgttctt ccaccgcacg atcgcgtagg cgagcttctg cggcagccac
     1081 gcccgcagcc gatcagcgat cgcgtcactg gcgggccgcg atgcgacata cgtcggtgag
     1141 cgctgcagca tcgtcacgtg cgcagcttgt tcggcaagcg agggcaccag cgtcacggct
     1201 gtggcgccgc tgccgatgac gacgacgcgc ttgccccgat agtcgaggtc ctccggccag
     1261 tgctgcggat gaatgacggg tcctcggaag cgttccacac cagggaattt cgggctgaag
     1321 ccctcgtcgt agcggtagta gcccgtgcag acgtacagga acgagcagct gatcgtgagc
     1381 agttcatcgg tgtcggtgcg atgcacgtcg acggtccagc gggcctcgtc gctgtcccaa
     1441 ttggcgttca ccacacggtg attcagccgg atgagcccgt cgacgtcgta ctgctcggcg
     1501 gtatcgtgga tgtagcgcag aatgctcggc ccgtcggcga tcgccttcgg gtcggtccac
     1561 ggcgagaacc ggtagcccag cgtgaacatg tccgagtccg atcggatgcc cgggtagcgg
     1621 aacaggtccc aggtgccgcc gatggcgtca cgtgcctcga gcacgacgac cgacttgtcg
     1681 gggcactcct ggcgcagctg gcacgcggcg ccgacgcccg agagcccggc gccgacgatc
     1741 aagacgtcca cggtttccgc tggtcgcgac attgcgttgc ctttcgtccg gcgtgctggt
     1801 acgactcagt accaggatcg gtactagacg gtaccactat tagacgcgtg tggaaagggg
     1861 gcttgcgacg ttcggtacta aggcgtaccg tgaccgggta ccagggggta ccaccaacgg
     1921 aggtgacata tggctgtggc gaccacggac ccggtgcgac tgccgcccgc accgcggata
     1981 ccgaagctcc tgcaaggcgt gggcttcgtc gccgcccggg agaaatcggt cgccgcgctg
     2041 gccaagcgat acgggtcggc cttcacgctg cggctgccga tcttcggcga gaccgtcgtg
     2101 gtcagcgatc cgggcttgat caaagatctg ttcaccacca attccgatct ggttggcagg
     2161 gcgggcgtgc tcggtgagat gttcggcccc ggctcgacat tcagtctgga cggcaccgag
     2221 caccgcgagc gtcgcaagct tctggtgccg ccgttccatg gcaagcggat ggcctcctac
     2281 gaaggcatca tcgaagagga ggtgttgcgc gagaccgcgt cgtggcccga gggcagggaa
     2341 ttcgaaacca tgccgtcgat gatgcgcatc acgctcaacg caatcctgcg gacggtgttc
     2401 ggcgcggagg gtgccgcgct cgacgaactg cgcgaggtgc tgccgagcct ggtgctgcac
     2461 gcctctcgac ttgccgtcct tccgccactt gtgcgacgcg acttcggcgc ctggagcccg
     2521 ggcgggaagc tgacggcgaa tcgcacgcgc tacgacgaga tcattgctcg actgatttcc
     2581 gacatccgca acgatccgaa gttcgacgaa cgcaacgacg tcctctcgtt gatgctgcag
     2641 gcccgctacg aggacggctc acagatctcc gacgaccaca tcgccgacga gctgctgacc
     2701 ctgctcgccg ccggccacga gaccacggcg acgacgctgg catggagcgt cgagcggctc
     2761 cgtcgccacc cgcgactgct ggcccgcttg cgcgccgagg ccgacgcggg aggttctgag
     2821 ctggtgcagg ccacgatctg ggaggtgcaa cgcacccgcc cggtcatcaa tggcaccgct
     2881 cggctgacga agacccggat ccggctgggg gagtgggtca ttccggaaca ccatgccatt
     2941 ctcgccagca tctcactcgc gcacgcgtcg gaggacagct tcggcgatgc gaaggcattc
     3001 aatcccgacc gattcgtcgg caacccgcca gacaactacg cgtggatccc gtacgggggc
     3061 ggagtgcgcc gctgtatcgg cgcggcgttc gcgaacgtgg agatgaacgt gactctgcgg
     3121 acattgctgc gcgagttcga attcggcacg acatacgctg cgggtgagcg cttgcactcg
     3181 cgcggggtcg ccaccgcgcc cgcacacggc ggtcgcgccg tggtgtatcg gcgaacgacc
     3241 aggtccctcg agcctacggg tcagacgcag cgagtgtcgg catgatcgcg cgcgaggagg
     3301 tgcttgtcga tgtcggccgc ggcatcgaac tgtgctacga ccagaccggc gatcccgcag
     3361 atccgccgat cctgctgatc gccggcctcg ggcagcaatt gcactcgtgg cccaacgatt
     3421 tcgtcgccgc gttggccggc cggggctatc gggtgacgcg gttcgacaac cgggatgcgg
     3481 ggcggtccac ccacatgaac tatccgccgc cgaacccggt ggcgatgttc cggggtcgca
     3541 accggtacca cctcggcgac atggcccgtg acaccgccgg actgctggat gcgctcggct
     3601 acgccgatgc ccatctggtc ggcatctcca tgggaggcat gatcgcgcag acggtcgccg
     3661 cgcactaccc gggccgggtt cgcacgttga cgtcgatcat gtcgaacacg ggcgcgccgc
     3721 ggctgggccg cccggcgttg tcgacctggc ggcggatgtt gacgtctcgc ccaccgcgca
     3781 cgcgggccga ggcgatggac cgagccgcga acatgttctc ccacatcggg tctcacggat
     3841 tcccgttcga cgagcaagcg gttcgggagt acgccgggat cgcgtgggac cgagacgcca
     3901 gccccgcggg cattgccagg cagctcgcgg gcattttcgc ctccggggac cgcaccgccg
     3961 aacttcgcaa catcgacgcg ccgaccctga tcatccacgg cgaccgcgac cggatggtgc
     4021 acccgaccgg tggatcggcg accgcgcgag cgatccccgg tgcccggctg gagaccatcg
     4081 tcggcctcgg ccacgatctg cccatcggcg catggggccg gatcatcgac ctgatcaccg
     4141 accacacgac cttcgccccc gttacccatt ccgaagagga gcacgcatga gataccgaag
     4201 acgacgacga gtgaggatcg cagcgaggaa cgagcgagga ccggagcgag gaggagtcga
     4261 ggcatgagca aggcccaacg ccgcaccatc agcggccgca ccgtcgttat caccggcgcg
     4321 gcatctggca tcggtcgggc actggcgcaa cgcctttcag cgcacagctg ccccgtcgcg
     4381 atcgccgaca tcgacgagaa cggactcaaa gagaccgagg ccacgctgct tgggccgacg
     4441 ctgctgcggg tactcgacgt tgctgacgcc gaagcgcagc gcgacttcgc agc
//
LOCUS       DASRJA010000307         9589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_729107,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000307 DASRJA010000000
VERSION     DASRJA010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9589
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729107"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFC01_13340"
     CDS             <1..386
                     /locus_tag="VFC01_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HZN80651.1"
                     /translation="ELGLAGIVATNTTVSRSGLATPGAEALGSGGVSGPPVAQRSAEV
                     LRRLYKRVGDRLVLISVGGIETADDAWDRITSGASLLQGYTGFIYGGGLWAKHIHDGL
                     ARRLHEGGFTSLADAVGSAIKREQT"
     gene            complement(463..993)
                     /locus_tag="VFC01_13345"
     CDS             complement(463..993)
                     /locus_tag="VFC01_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZN80652.1"
                     /translation="MAFDYNPYEFLPKLPSFTLTSESLTDGRPLANDQVSGIMGAGGS
                     DVSPQLSWSGFPEETRSFAVTVYDPDAPTASGFWHWAVANLPATVTDLPAGAGDGSGL
                     PGDALTLANDAGLKRYIGAAPPPGHGVHRYFVAVHAVKVEKLELDGDASPAYLGFNLF
                     MNAIARAVIQGTYEQK"
     gene            complement(1018..2352)
                     /locus_tag="VFC01_13350"
     CDS             complement(1018..2352)
                     /locus_tag="VFC01_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZN80653.1"
                     /translation="MTGHLNAEAEVVDLVSALIRFDTSNTGEPQTTKGEAECARWVAE
                     QLEEVGYATEYVDAGAPGRGNLFARLSGANSDRGALLLHGHLDVVPAEASDWSVHPFS
                     GAVEDGYVWGRGAVDMKDMCGMLIAIARHFKRSGIVPPRDLVFAFVSDEEAGGKYGSQ
                     WLVDNRPDLFEGVTEAIGEVGGFSLTVPRKDGGERRLYLVETAEKSMMWMKLSVRSHA
                     GHGSMIHDHNAVTAVAEATAKLGRHEFPLVMTDAVGQFLAAITEETGYEFDLDSPDLP
                     GTIAKLGPIARVVGATLRDTANPTMLKAGYKANVIPATAESVVDCRILPGRQAAFERE
                     VDELIGPDVTREWITELPSYETTFDGDLVDAMNNAILSVDPDARIVPYMLSGGTDAKA
                     FARLGIRCFGFAPLQLPPDLDFTALFHGVDERVPVDALKFGTQVLEHFLLHC"
     gene            2434..2522
                     /locus_tag="VFC01_13355"
     tRNA            2434..2522
                     /locus_tag="VFC01_13355"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2467..2469,aa:Leu,seq:gag)
     gene            complement(2528..3346)
                     /locus_tag="VFC01_13360"
     CDS             complement(2528..3346)
                     /locus_tag="VFC01_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80654.1"
                     /translation="MRLAGQVAIVTGGALGIGGGISRRLAAAGAHVVLADINADAASA
                     ARDEMVAAGGRCTTIVGDIRYRDVVAKVTETALAIDDSRIDVLVNNVGDFRPAARDFL
                     HSTEEQWQTLYELNLLHVLRMCHAVLPAMVKRGRGAIVNNATVEAFRGIPYAAPYAAF
                     NAGVVAFTRSLAVDVAQHGIRVNAIAPDLADTPQTPAELMLRGRDADLIRTWIPVGRF
                     GRPDDYAAVVEFLASDDARFVTGQTIPVDGGTLAASGWYARADGKGWTNMPNEA"
     gene            complement(3363..7373)
                     /locus_tag="VFC01_13365"
     CDS             complement(3363..7373)
                     /locus_tag="VFC01_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nitrate reductase/sulfite reductase
                     flavoprotein subunit alpha"
                     /protein_id="HZN80655.1"
                     /translation="MAVTRTACSYCGVGCGIEVQTTTDPDSGKPVIARVSGDKLHPTN
                     FGRLCTKGATHAEMMAADDGRLKSALLRPTRTDEPVPTPVDDAVAEAGRRLRAIIDEH
                     GQDAVALYVSGQMSIEAQYLATKLAKGFIQTVHIESNSRLCMASAGTGFKQSLGADGP
                     PGSYTDFDCTDLFFVIGSNMADCHPILYLRMADRLKAGAKLIVVDPRRTTTAEHADLF
                     LQIKPGTDLALLNGLLHLLVENGDIDEGFIAAHAEGWDAMPAFLADYPPDRVAQITGL
                     AESDIRTAARMIADAGEWMSCWTMGLNQSTHGTWNTNAICNLHLATGAICRPGSGPMS
                     LTGQPNAMGGREMGYMGPGLPGQRSVLSADDRAFVEEQWGLPAGTIRSDVGPGTIDMF
                     EQLADGTIKACWIICTNPVATVANRKTVITGLETAQLVITQDAYRTTATNHYADIVLP
                     ATLWAESDAVMVNSERNLTLMQQSIPQAGQARPDWELICDVAAHMGFAEDFDYKSSEE
                     IFDEIRRFANPKTGYDLRGASYERLRRTPLQWPCPPDDEDRHPIRYLNDGVSQELFVD
                     EHGHSPRLAFPTPSRRAVFHARPHMDPREMPDDDFPLVLNTGRLQHQWHTMTKTGKVA
                     KLNKLDSGPFVEIHPVDAVALDIVSGQPVELTSRRGRAVLPAVVSDRVRPGNCFVPFH
                     WNDEHGEYLTINALTNDAVDPDSLQPEFKVCAVSLRPVKSVKSVDPVVEELDFGQPKP
                     MLTDDEKIYLAAFFSGLAEGVSGVPVLPASAPVAPGVRLWIDGLLAGRYSRIAAAPTE
                     QPVNDGPLVLWASQTGNAEEFAARLAGRLGGSQLVNMDEMQLGELAAARDVLVITSTF
                     GDGGPPDNGAGFWDRLHSAEAPALDGIRYAVLGIGDRSYANFCGHAKAIDGRLAALGG
                     TRMLERTECEAYDDEPMDRWADQVTALVSVPTEPVRTNGGGTSTVARPTKAAEPFTRA
                     KPLLARLARNVVLTAPTSRKEVRQFGFDVSEYEVSYAVGDSLGVYATNDPETVDAWLA
                     ATGLRGDTSVEVDGNEQSLRDALIVSYDICRVTPNLLRFVAESCRDKAAAKTLMAPKD
                     KRDKWLVDRNGLDIVQEFAVRAEPEEWQEVLVRLTPRNYSISSSPLISPHEVQVTVSV
                     VRYRGADGGQRGGVCSTFLADRAAAAPVFLQRSPHFRPPEDGDIPMIMVGPGTGIAPF
                     RGFLQERRALGHAGRNWLFFGDRHRSENFYYRDDFEDMVRDGFLNRLDLAFSRDQADR
                     VYVQHKMLDYGADVWRWLDEGAHFYVCGDASRMAKDVDAALTAVIEKHGGMSPEKAHD
                     YKRELVASKRYVRDVY"
     gene            complement(7484..8908)
                     /locus_tag="VFC01_13370"
     CDS             complement(7484..8908)
                     /locus_tag="VFC01_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HZN80656.1"
                     /translation="MTILSRDRNIEHWDAEDVEAWDAGGAKVAKRNLIWSIFAEHIGF
                     SVWSIWSVMVLFMPQNVYHIDAAGKFYLVAVPTLVGAAMRIPYTIAPAKFGGRNWTIV
                     SALLLLIPTVLTWYVMKQPGTSYTTFMVVAAFAGFGGGNFASSMTNINAFYPQRLKGW
                     ALGLNAGGGNIGVPVIQLVGLLVIAAVSNTRPEIVCAIYLVLIALAAFGAALFMDNLG
                     NQRSNLGAMVEALRYKHSWVMSFLYIGTFGSFIGFSFAFGQVLQINFLAGGDNAAQAS
                     LHAAQIAFVGPLLGSISRPFGGKLSDRIGGGKVTLYVFIAMIFAAGILVVTGMIDDGM
                     KGAATGVQMTAYVVGFILLFILSGLGNGSTYKMIPSIFEAKAQGHDEWSRDEKAAWSR
                     SMSGALIGFAGAIGALGGVFINVVLRASYVGAAKSATNAFWVFLGFYVICAIVTWFVF
                     LRIQSARAGAGEEIGRAAAPVPAT"
     gene            9092..9445
                     /locus_tag="VFC01_13375"
     CDS             9092..9445
                     /locus_tag="VFC01_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HZN80657.1"
                     /translation="MSDGSTPRFLQGAFEFEGNGLDKPSLIDDSLRYVVPAGAVTQPV
                     YFRGGNSTKQMITVVLFRDGKPMRYFPIAAKGATHVALRVVEDLLGDTVLELHVAAPS
                     GCKGTLVVDLGLVEI"
BASE COUNT         1644 a         3164 c         3132 g         1649 t
ORIGIN      
        1 tcgaattggg cttggcaggc atcgtcgcga cgaataccac cgtgtcgcgc tcaggactcg
       61 cgacacccgg cgccgaggcg ctgggctccg gcggcgtttc cggtccgcct gtcgcgcagc
      121 gctccgcgga agtgttgcgc cggctgtaca aacgcgtggg tgatcggctc gtgctgatca
      181 gcgtcggcgg catcgagacc gccgacgacg cgtgggaccg catcacgtcc ggcgcatcac
      241 tgctgcaggg ctacaccggc ttcatctacg gcggtgggtt gtgggccaag cacattcatg
      301 acgggctggc gcgccgactg cacgagggcg gctttacgtc actggccgac gccgtcggct
      361 cagccatcaa gcgcgagcag acgtaaaaac acccgacacg ccgagaaaat ggggaccttt
      421 gcgttcccca gctgacgcgt gggcccaact cgcgtaggaa agctacttct gctcatacgt
      481 gccctggatg accgcgcggg cgatcgcgtt catgaacagg ttgaagccca ggtaagccgg
      541 actggcgtct ccgtccagtt cgagcttctc gaccttcacg gcgtgcactg cgacgaaata
      601 ccggtgcaca ccatggccgg gcggcggggc ggcgccgatg tagcgcttga ggccagcgtc
      661 gttggccaaa gtcagtgcat cgccgggcaa tccgctgccg tcgccggcac cggcgggcag
      721 gtccgtgacg gtcgcgggca ggttggcgac ggcccaatgc cagaagccag acgccgtcgg
      781 tgcgtccggg tcatacaccg tgacggcgaa actgcgcgtc tcctcgggaa atccggacca
      841 ggacagctgc ggcgacacgt cggacccgcc cgcgcccatg atgccgctga cctgatcgtt
      901 ggccaacggc cgcccgtcgg tgagcgactc cgacgtcaac gtgaacgacg gcagctttgg
      961 caggaactca taggggttgt agtcaaaggc catgctcttt cctttctttc tttgtggtca
     1021 gcagtgcagc aggaaatgct cgagaacctg cgtaccgaac ttcaaggcat cgacgggtac
     1081 ccgctcgtcg acgccgtgga acaaggcggt gaaatccaga tccggcggca gttgcagcgg
     1141 cgcgaacccg aagcagcgaa tccccaagcg ggcgaaggcc tttgcatcgg taccgccgga
     1201 cagcatgtac ggcacgatgc gggcatcggg atcgaccgac agaatcgcgt tgttcatggc
     1261 gtccaccagg tcgccgtcaa acgtcgtttc gtacgacggc agttcggtga tccactcccg
     1321 cgtcacgtcg gggccgatca actcgtcgac ctcgcgctcg aaggcggcct ggcggcccgg
     1381 caggatccgg cagtcgacca ccgactccgc ggtggccggg atgacgttgg ccttgtagcc
     1441 ggccttgagc atcgtcgggt tggcggtatc acgcagggtc gcgccgacca cgcgtgcgat
     1501 cggaccgagc ttggcgatgg tgccgggcag gtcgggtgaa tccaggtcga attcgtagcc
     1561 ggtttcctcg gtgatcgcgg caaggaactg tccgacggcg tcggtcatca ccagcgggaa
     1621 ttcgtggcgg ccgagcttgg cggtggcctc tgcgaccgcg gtgaccgcgt tgtggtcgtg
     1681 gatcatcgag ccgtgaccgg catggctacg gacgctgagc ttcatccaca tcatcgactt
     1741 ctcggcggtt tcgaccaggt acaggcggcg ttcaccgccg tctttgcgcg gcacggtcag
     1801 cgagaaccca ccgacttcgc cgatcgcctc ggtgacgccc tcgaacaggt cgggtcggtt
     1861 gtcgaccagc cactgcgacc cgtacttccc gccggcctcc tcatcggaga cgaacgcgaa
     1921 caccagatcg cgcggcggca cgatgcccga ccgtttgaaa tgccgggcga tcgcgatgag
     1981 catgccgcac atgtccttca tgtcgacggc gccgcggccc cacacgtagc cgtcctccac
     2041 ggcgccggag aacgggtgca cgctccaatc cgacgcctcg gcgggcacca cgtcgaggtg
     2101 accgtgcaac agcagggcgc cccggtcgga attggctccg gagagcctgg cgaacaggtt
     2161 gccccggccc ggcgcgccgg cgtccacgta ttcggtcgcg tacccgacct cttccagctg
     2221 ttcggccacc cagcgagcgc attcggcctc gcctttggtg gtttgcggtt caccggtgtt
     2281 ggaggtgtcg aagcggatga gcgcgctgac gaggtcaacc acctcggctt cggcgttgag
     2341 gtgaccagtc acgccaccat tcgtaccatt gaggcggccg ctgggtttgg gtccgggctg
     2401 cccgatccga tagccttagc tgcccacgaa ctggtccgag tggcggaatg gcagacgcgc
     2461 tagcttgagg tgctagtgcc ctattaacgg gcgtgggggt tcaagtcccc cctcggacac
     2521 cacactttca ggcttcgtta ggcatattcg tccagccctt gccgtcggca cgggcatacc
     2581 acccggatgc ggccagggtg ccaccgtcca ccggaatcgt ctgcccggtg acgaagcgcg
     2641 cgtcgtcgga cgccaggaat tccacgaccg ccgcgtagtc gtcgggccgg ccgaaccggc
     2701 ccaccgggat ccaggtcctg atcaagtcgg cgtcgcggcc ccgcagcata agctcggcgg
     2761 gcgtctgcgg tgtatcggcg agatcggggg cgatcgcgtt gacgcgaatg ccgtgttgcg
     2821 cgacgtcgac ggcgaggctg cgggtgaacg cgaccactcc cgcgttgaac gccgcgtagg
     2881 gggcggcgta cggaatgccc cggaacgcct ccacggtcgc gttgttgacg atcgcgccgc
     2941 ggccacgttt caccatcgcg ggcagcacgg cgtgacacat gcgaagcacg tgcagcaggt
     3001 tcagttcgta cagcgtctgc cactgctcct cggtgctgtg caggaaatcc cttgccgcgg
     3061 ggcggaagtc gccgacgttg ttcaccagga cgtcgatccg tgaatcatca atggccagtg
     3121 cggtttcggt cactttggcc accacgtcgc ggtagcggat gtcaccgacg atggtcgtgc
     3181 agcgcccgcc cgccgcaacc atttcgtcgc gtgcagccga ggcggcgtcg gcattgatgt
     3241 cggcaagcac aacgtgtgcg cctgccgccg ccaggcgcct actgatgccg ccgccaatgc
     3301 ccaacgcgcc gccggtgacg atggcgacct gtcccgctaa ccgcatccgg acagcgtatt
     3361 ggctagtaca cgtcgcgcac gtagcgtttc gacgccacca gctcacgctt gtagtcgtgc
     3421 gccttctcgg gcgacatgcc gccgtgtttc tcgatgaccg ccgtcagcgc cgcatcgacg
     3481 tccttcgcca tcctgctggc gtccccgcac acgtagaagt gcgcgccctc gtcgagccac
     3541 cgccacacgt ctgcgccgta gtcgagcatc ttgtgctgga catagacccg gtcggcctga
     3601 tcgcgggaga acgccagatc cagccggttc aagaagccgt cgcggaccat gtcctcgaaa
     3661 tcgtcgcggt agtagaagtt ttcgctgcgg tgccggtcgc cgaagaacag ccagttgcgg
     3721 ccggcgtggc cgagcgcgcg ccgctcctgc agaaagccgc ggaacggggc gatgccggtg
     3781 ccgggcccga ccatgatcat cggtatgtcg ccgtcctcgg gcggacggaa atgcggtgag
     3841 cgttgcagga ataccggcgc cgcggccgcc cggtcggcca ggaatgtcga gcacaccccg
     3901 ccgcgctggc cgccgtcggc gccgcggtag cgcaccaccg acaccgtcac ctgcacctcg
     3961 tgcgggctga tcagcgggct cgacgagatc gagtagttgc gcggggtcag tcgcaccagc
     4021 acttcctgcc actcctcggg ctcggcccgg accgcgaact cctgcacgat gtcgagcccg
     4081 ttgcggtcca ccaaccactt gtcgcgcttg tccttgggcg ccatcagcgt cttggcggcg
     4141 gccttgtccc ggcaggattc cgcgacaaag cgcagcaggt tgggcgtcac ccggcagatg
     4201 tcgtaggaca cgatgagcgc gtcccgcagg ctctgttcgt tgccgtccac ctcgacggac
     4261 gtgtcgccgc gcaacccggt ggccgccagc caggcgtcga ccgtctcggg atcgttggtg
     4321 gcatagaccc ccagtgagtc gccgaccgcg tagctgacct cgtattcgga gacgtcaaag
     4381 ccgaactggc gcacttcttt tcgcgatgtc ggggcggtca gcacgacatt gcgggccagc
     4441 cttgccaata gcggcttggc ccgggtgaac ggctcggccg ccttggtcgg cctggcaacc
     4501 gtcgacgtac cgccaccgtt ggtccgcacc ggctcggttg gcactgagac gagggcggtg
     4561 acctggtcgg cccaccgatc cattggctcg tcgtcgtagg cttcgcactc ggtgcgttcc
     4621 agcatccgcg tcccgcccag cgccgcgagc ctgccatcga tcgccttcgc gtggccgcag
     4681 aaattcgcat acgaccggtc gccgatgccg agcaccgcgt accgaatccc atccaacgca
     4741 ggcgcctcgg ccgaatgcag gcgatcccag aatccggcgc cgttgtccgg cggcccgccg
     4801 tcaccgaacg tgctggtgat caccaacacg tcgcgcgcag ccgccagctc ccccagctgc
     4861 atttcgtcca tgttgaccag ctgggagccg ccaagccggc cggccagccg ggctgcgaac
     4921 tcctcggcgt ttccggtctg cgacgcccac aacaccagcg gtccatcgtt gaccggctgt
     4981 tcggtgggag ccgccgcgat gcgcgagtac cgaccggcca acagaccgtc gatccacagc
     5041 cgaacaccgg gtgcgaccgg cgctgacgcg ggcagcaccg gtacaccgct gacgccctcc
     5101 gccagaccac tgaaaaacgc cgcaaggtag atcttttcgt catcggtcag catcggcttt
     5161 ggctgaccga agtcgagttc ctcgaccacc ggatccaccg acttcaccga cttcaccggc
     5221 ctcaggctca ccgcgcacac cttgaactcg ggttgcagcg agtcggggtc gacggcgtcg
     5281 ttggtcagcg cattgatcgt gaggtactcg ccgtgctcgt cgttccagtg aaacggcaca
     5341 aagcaattgc ccggccgcac ccggtcgctg acgactgcgg gaagcaccgc cctgccccgc
     5401 cgcgacgtca gctcgacggg ctggccgctc acgatgtcga gcgcgacggc gtcaaccggg
     5461 tggatttcca caaacggtcc gctatcgagc ttgttgagct tggcgacctt gccggtcttg
     5521 gtcatcgtgt gccattgatg ttgcagccga ccggtattga gcaccagcgg gaagtcgtcg
     5581 tcgggcatct cacgcggatc catgtgcggc cgggcgtgaa acaccgcgcg ccgcgacggg
     5641 gtcggaaacg cgagccgtgg cgaatggccg tgctcgtcga cgaacagctc ttggctgaca
     5701 ccatcattga ggtagcggat cgggtgccgg tcctcgtcat ccggcgggca gggccactgc
     5761 agcggcgtcc gacgcagccg ctcatagctc gcgccgcgga ggtcgtaacc ggtctttggg
     5821 ttggcgaacc ggcggatctc gtcgaagatc tcctcgctgg acttgtagtc gaagtcctcc
     5881 gcgaacccca tgtgcgcggc gacgtcgcag atcagctccc agtcgggccg cgcctgaccg
     5941 gcctgcggga tggactgctg catcagcgtc aggttgcgct cggaattgac catcacggcg
     6001 tcggactcgg cccacagcgt ggcgggcaac acgatgtccg cgtagtggtt ggtcgcggtg
     6061 gtgcggtagg cgtcctgggt gatgacgagc tgtgcggtct cgagaccggt gatgaccgtc
     6121 ttccgattcg ccacggtggc aacgggattg gtgcagatga tccagcacgc cttgatggtg
     6181 ccgtcggcga gttgctcgaa catgtcgata gtgccgggac cgacgtcgga gcgaatggtt
     6241 ccggcgggca gcccccactg ctcctcgacg aacgcccggt cgtcggccga cagcacggaa
     6301 cgctgccccg gcaaaccggg ccccatataa cccatttcgc gtccgcccat cgcgttgggc
     6361 tgcccggtca gcgacatcgg accgctgccc ggtcggcaga tcgcaccggt ggccagatgc
     6421 aggttgcaga tcgcgttcgt gttccaggtg ccgtgcgtgc tctggttgag gcccatcgtc
     6481 cagcagctca tccactcccc cgcgtcggcg atcatccggg cggcagtgcg gatgtcggac
     6541 tcagcgagac cggtgatctg agcgacccgg tccggcggat agtcggcaag gaacgcaggc
     6601 atggcgtccc agccttcggc gtgcgctgcg atgaaacctt cgtcgatatc cccgttttcg
     6661 acgagcaggt gcagcagacc gttcagcagt gccagatcgg tgcccggctt gatctgcagg
     6721 aacaggtcgg cgtgttcggc cgtggtggtg cgccgggggt cgacgacgat cagcttcgcg
     6781 cccgccttga gccggtcggc catccgcaga tacaggatgg gatggcagtc cgccatgttc
     6841 gagccgataa cgaaaaacaa atccgtgcaa tcgaagtcgg tatacgaccc cggcgggccg
     6901 tccgcgccga gtgactgctt gaatccggta cccgcgctgg ccatgcacaa ccgcgagttc
     6961 gattcgatgt ggactgtttg gatgaagccc ttggccagct tggtggccaa atactgcgcc
     7021 tcgatggaca tctggccgga gacgtagagc gcgacggcat cctgaccgtg ctcgtcgatg
     7081 atcgcgcgta accggcggcc ggcctcggcg acggcgtcgt cgacgggtgt gggaacgggt
     7141 tcgtcggtgc gtgtcgggcg cagcagggcc gacttcaagc ggccgtcgtc ggcggccatc
     7201 atctcggcgt gtgtggcgcc ttttgtgcac agtcggccga agttcgtcgg gtgcagtttg
     7261 tccccggaga ctcgggcgat gacgggcttg cccgagtctg gatccgtggt ggtttgcact
     7321 tcgatgccgc agccgacgcc gcaatacgag cacgccgtcc gggtcaccgc catgggccca
     7381 tggtgggcct gcaatgtttc aggctgtttt ccgacgacgt tatcgcggtg aaaattgatg
     7441 ctcaccgggc gtgtcgccgc ccggtgagca tcaaatccgc aggtcaggtg gctgggacgg
     7501 gggccgcagc gcgaccgatt tcctcgcccg cgccggccct ggccgattgg atccgcagga
     7561 acacgaacca ggtgactatg gcgcagatca catagaagcc gagaaagacc cagaatgcgt
     7621 tggtcgccga cttcgccgcg ccgacgtacg acgcccgcag caccacgttg atgaacaccc
     7681 cgccgagcgc gccgatcgcg ccggcgaaac cgatcagcgc gccggacatg ctgcgtgacc
     7741 acgccgcctt ctcatcgcgg ctccactcgt cgtgcccctg cgccttggcc tcgaaaatcg
     7801 acgggatcat cttgtacgtc gacccgttgc caagacccga cagaatgaac agcaggatga
     7861 aaccgaccac gtaggcagtc atctgcacac cggtcgctgc gcccttcatc ccatcgtcga
     7921 tcatcccggt caccaccagg atgcccgccg cgaagatcat cgcgatgaac acgtacagcg
     7981 tcaccttgcc gccaccgatg cggtcggaca gcttgccgcc gaacggccgg gagatggagc
     8041 cgagcagcgg tccgacgaac gcgatctgcg ctgcgtgcag cgacgcctgc gcagcgttgt
     8101 cgccgccggc aaggaagttg atctgcagca cctggccgaa tgcgaacgag aagccgatga
     8161 acgagccgaa cgtgccgatg tagaggaagc tcatcaccca ggagtgcttg taccgcaacg
     8221 cctcaaccat cgcgcccagg ttcgaccgct gattgccgag gttgtccatg aacagcgccg
     8281 caccaaatgc ggccagcgcg atcagcacaa ggtaaatggc acaaacgatt tcgggtcgag
     8341 tgttgctcac cgccgcgatc accagcaagc ccacgagttg gatgaccggg acgccgatgt
     8401 tgccgccgcc cgcgttcaga cccagcgccc agcccttgag ccgttgcggg tagaacgcat
     8461 tgatgttcgt catcgacgag gcgaagttac cgccgccgaa tccggcgaac gccgcgacga
     8521 ccatgaacgt cgtgtaggac gtgcccggct gcttcatcac ataccacgtc agcaccgtag
     8581 gaatgagcag caacagtgcg ctgactattg tccagttgcg gccgccgaac ttggcaggcg
     8641 cgatcgtgta ggggatgcgc atcgccgcgc cgaccagggt gggcaccgcg acgaggtaga
     8701 acttgcccgc cgcgtcgatg tgatagacgt tctgcggcat gaacagcacc atcaccgacc
     8761 agatcgacca cacggagaag ccgatgtgct cggcgaagat ggaccagatc aggttgcgtt
     8821 tggcgacctt ggcgccgccg gcgtcccaag cttcgacgtc ttcggcgtcc cagtgctcga
     8881 tgttgcgatc gcgggacaag attgtcatgc gggcctcccc tgcagtagtt gaaataggtc
     8941 tactgccaac ccgttccgcg tcaattgccc cgccgtatcg atattgtcaa cacccgctca
     9001 cttcggcgaa acacgtgcgc gtgcgcaatg ggattcacgg ctgactcatc gacgaaacgg
     9061 ccgcgaaaca aacgcgcctt agcttccgag ggtgagcgac ggcagtactc cccgattcct
     9121 gcagggtgca ttcgagtttg agggcaacgg cctggacaag ccgtcgctga tcgacgactc
     9181 gctgcgctat gtcgtgccgg cgggcgccgt gacgcagccg gtgtacttcc gcggcggtaa
     9241 ctccaccaaa caaatgatca ccgtcgtgct gttccgcgac ggcaagccga tgcgctactt
     9301 cccgatcgcg gcaaagggcg ccacccatgt ggccctgcgg gtggtcgaag acctgctcgg
     9361 cgacacggtg ctggagttgc acgtcgccgc cccgagcgga tgcaaaggca cgctggtcgt
     9421 cgatctcggt ctggtggaaa tctgacgagc gcttgcgcga ggaaacgagg gcactgatga
     9481 agcagaagct ggtcgtcgtc ggcaacggca tggccggcgt gcgcgcgatc gaggaggtcc
     9541 tggcccgcgg cggcggcgag caattcgaca tcaccgtctt cggtgacga
//
LOCUS       DASRJA010000308         3445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_46796,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000308 DASRJA010000000
VERSION     DASRJA010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46796"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFC01_13380"
     CDS             <1..317
                     /locus_tag="VFC01_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZN80658.1"
                     /translation="EGFMRCHGLQCGFCTPGMMITARALLDRVPNPDEETIREAISGQ
                     ICRCTGYTTIVRSIQWAATHSDGRTVESGPSDSEFAPESQTETTHGGEGAEPLLEAGS
                     SS"
     gene            314..2722
                     /locus_tag="VFC01_13385"
     CDS             314..2722
                     /locus_tag="VFC01_13385"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HZN80659.1"
                     /translation="MTTVETREMTGPSPEDIVNNDQKPCGHGRMLRKEDPRFIRGKGA
                     YVDDVQLSGMLHLAVLRSPFAHARIVSIDTTAAQAHPKVKAVVTGADLAEKGLAWMPT
                     LSNDVQAVLATDKVRFQGQEVAFVVAEDRYAARDALELIDVEYDPLEPVIDVRHALDE
                     SAPVIRSDLDGKTDNHIFDWETGDSAATDAVFTKADVVVKQEIVYPRVHPAPMETCGA
                     VADLDPVTGKLTLWSTSQAPHAHRTLYALVAGLPEHKIRVISPDIGGGFGNKVPIYPG
                     YVCAIVGSLLLGKPVKWMEDRSENLTSTSFARDYIMVGEIAATNDGKILAIRSNVLAD
                     HGAFNGQAAPMKYPAGFFGVFTGSYDIEAAYCHMTAVYTNKAPGGVAYACSFRITEAV
                     YFVERLVDCLAHEMKMDPAELRLRNLLKPDQFPYKSKTGWVYDSGDYETTMRKAMDMI
                     GYDALRAEQKERRARGELMGIGMSFFTEAVGAGPRKNMDILGLGMADGCELRVHPTGK
                     AVVRLSVQTQGQGHETTFAQIVAEELGIPPDDIDVVHGDTDQTPFGLGTYGSRSTPVS
                     GAAAALVARKVRDKAKIIASGMLEASVADLEWEKGSFHVKGDPSASVTIQDIAMRAHG
                     AGDLPEGIEGGLDAQICYNPENLTYPYGAYFCVVDIDPGTAVVKVRRFLAVDDCGTRI
                     NPMIIEGQVHGGIVDGIGMALMEMIAFDEEGNCLGGSLMDYLIPTALEVPQLETGYTV
                     TPSPHHPIGAKGIGESATVGSPPAVVNAVVDALAPFGVRHADMPLTPSRVWEAMQGRA
                     TPPI"
     gene            2733..>3445
                     /locus_tag="VFC01_13390"
     CDS             2733..>3445
                     /locus_tag="VFC01_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005243651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZN80660.1"
                     /translation="MTMIERAKQLRRSRTPFVHATVVRAQQPASAHAGDEAILLSDGT
                     IEGFVGGQCAQSSVRKAALGALQAGESVLLRVLPDGDVHFPEAPGACVVVNPCLSGGA
                     LEIFLEPQVPAPLVCICGATPIAQALAAMTAALGYEVRCDGDADVTQAAAVVIATHGG
                     PEAEIIRAALDAGVRYVGLVASKVRGGAIVDALALTDAERRRVHTPVGLPIGAKTPAE
                     IAVSILAEVVRAIRVEGLAA"
BASE COUNT          627 a         1093 c         1172 g          553 t
ORIGIN      
        1 aggaaggctt catgcgctgc cacgggctgc agtgcggatt ctgtacgcca gggatgatga
       61 tcacggcgcg agccctcctc gatcgcgtac ccaaccccga cgaggaaacc atccgtgagg
      121 ccatttccgg acagatctgt cgctgcaccg gctacaccac gatcgtgcga tccatccagt
      181 gggcagccac ccattccgac gggcgcaccg tcgaatccgg gccgtccgac agcgaattcg
      241 cgccggagtc gcagacagag accactcatg gcggcgaggg tgccgagccg ctgctggagg
      301 ccgggagcag ctcatgacca ctgtcgaaac ccgcgagatg accgggccga gcccggaaga
      361 catcgtcaac aacgaccaaa agccgtgcgg ccatggccgg atgctccgca aggaggatcc
      421 acggttcatc cgaggcaaag gcgcatacgt cgacgatgtg cagctgtccg gcatgttgca
      481 tctggcggtg ctgcgatcac cgttcgccca cgcccgcata gtgagcatcg atacgactgc
      541 ggcacaggcg catccgaagg taaaggccgt cgtgaccggc gccgatctgg cggaaaaagg
      601 tttggcgtgg atgccgaccc tgtccaacga cgtgcaggcc gtgctggcca ccgacaaggt
      661 ccgcttccag ggccaggagg tggccttcgt ggtcgccgaa gatcgctatg ccgcacgtga
      721 tgcgttggag ctcatcgacg tcgagtacga tccgttggag cccgtcatag atgtgaggca
      781 cgcgctcgac gagtcagccc cggtgatccg cagcgacctg gacggcaaga ccgacaacca
      841 catcttcgac tgggagaccg gtgactccgc cgcgaccgat gcggtgttca cgaaggccga
      901 tgtcgtcgtc aagcaggaaa tcgtgtaccc gcgagtgcat cccgcgccga tggagacctg
      961 cggagcggtc gccgatctgg acccggtcac cggcaagctg acactctggt cgacctcgca
     1021 ggcaccacac gcccaccgca cgctgtacgc gttggtcgcc gggctgcccg agcacaagat
     1081 ccgggtgatc tcaccggaca tcggcggcgg cttcggaaac aaggtgccga tctatccggg
     1141 ctacgtgtgc gcgatcgtcg ggtcactgct gctgggcaag ccggtgaagt ggatggagga
     1201 ccgcagtgag aatctgacca gcaccagctt tgcccgcgac tacatcatgg tcggcgagat
     1261 cgccgccacg aacgacggca agattctggc gatcaggtcg aatgtgctcg ctgaccatgg
     1321 tgccttcaac gggcaggccg cgccgatgaa gtatccggct gggttcttcg gcgtcttcac
     1381 cggcagctat gacatcgagg ccgcctactg tcacatgacc gcggtgtaca ccaacaaggc
     1441 gcccggaggg gtggcctacg cgtgttcgtt ccgcatcacc gaggcggtgt atttcgtgga
     1501 gcggcttgtg gactgcctgg cccacgaaat gaagatggat ccggccgagc tgcgactgcg
     1561 aaacctcttg aagccagacc agtttccgta caagtccaag accggctggg tctacgactc
     1621 cggcgactac gagaccacca tgcgcaaggc catggacatg atcggctacg atgcattgcg
     1681 cgccgagcag aaggagaggc gcgcgcgtgg tgaattgatg ggcatcggta tgtcgttctt
     1741 caccgaggcg gtcggcgcag ggccgcgcaa gaacatggac atcctcggtc tgggtatggc
     1801 ggacggctgc gagctgcggg tgcacccgac cgggaaggcc gttgtgagac tgtcggtcca
     1861 gacgcagggc cagggccacg agacgacgtt cgcgcagatc gtcgccgagg agctcggcat
     1921 cccgcccgac gacattgacg tcgtgcacgg cgacaccgac cagacgccgt ttgggctggg
     1981 cacctatggc agtcggtcca cacccgtgtc tggggccgcc gccgcactgg tcgcgcgcaa
     2041 ggtcagggac aaggcgaaga tcatcgcatc cgggatgctg gaggcgtcgg tcgccgactt
     2101 ggagtgggag aaggggtcat tccacgtcaa gggcgatccg tcggcgtcgg tgaccatcca
     2161 ggacatcgcg atgcgtgcac acggcgcggg cgacttgccg gagggcatcg agggtggcct
     2221 ggacgcccag atctgttaca acccggagaa tctcacgtac ccgtacggtg cgtacttctg
     2281 cgtggtcgac atcgacccgg gcaccgcggt ggtgaaggtg cggcggttcc tcgcggtcga
     2341 cgactgcggc acacggatca atccgatgat catcgaggga caggtgcacg gcgggatcgt
     2401 cgacggcatc gggatggcgc tgatggagat gatcgcgttc gacgaagaag gcaactgcct
     2461 cggcgggtcg ctgatggact acctgatacc gaccgcgctg gaggtcccgc aattagagac
     2521 cggttacacc gtcactccgt cgccccatca tccgatcggt gcgaagggca tcggcgaatc
     2581 ggcaacggtc ggctcgccgc ctgcggtcgt caatgccgtg gtggatgcgt tggcgccgtt
     2641 cggggttcgg cacgccgaca tgccgctgac cccgtcgcgg gtgtgggagg ccatgcaggg
     2701 cagagccaca ccgccgatct agaggagcaa gtatgacgat gatcgaacgg gccaagcagt
     2761 tgcggcggtc gcggacgccg ttcgtccacg ccaccgtggt gcgcgcacag cagccggcct
     2821 ccgcgcatgc cggcgatgaa gcgatcctgc tgtcggacgg caccatcgaa ggctttgtcg
     2881 gtggccaatg cgcgcagagc tcggttcgca aggcggcact cggcgcgctg caagcagggg
     2941 agagtgtgct gctgcgcgta ttgcccgacg gcgacgtcca ctttccggag gcgccgggcg
     3001 cctgtgtggt cgtcaaccca tgcctgtccg gcggtgcgtt ggagatcttc ctcgaaccgc
     3061 aggtacccgc gccgctggtc tgcatctgcg gtgccacgcc gatcgcacag gcgctggccg
     3121 ccatgaccgc cgctctcggt tacgaggtgc gatgcgacgg tgacgccgac gtgacacagg
     3181 ccgccgccgt cgtcatcgcc actcacggcg ggcccgaggc cgagatcatc cgcgcggcac
     3241 tcgacgcagg cgtgcgctac gtaggcctgg tcgccagcaa ggtccgcggc ggtgcgatcg
     3301 ttgacgcgct ggcgctcacc gacgccgaac ggcgacgcgt gcacaccccc gtcgggctgc
     3361 ccatcggcgc gaagacgccc gccgagatcg cagtgtccat cctcgccgag gttgtccgcg
     3421 ccatccgggt ggaaggcctt gccgc
//
LOCUS       DASRJA010000309         8221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4079638,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000309 DASRJA010000000
VERSION     DASRJA010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8221
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4079638"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VFC01_13395"
     CDS             complement(<1..685)
                     /locus_tag="VFC01_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZN80661.1"
                     /translation="MRAVVFDAVGSPLRVTEIAPPECPADGAVIDVKATGICRSDWHA
                     WRGHEVVSLPHVPGHEFSGVVTKTGSQVSAFAVGDRVTVPFVNGCGQCDWCMAGQAQV
                     CPHQTQPGFSQAGSFAEQVAIPAADFNLVRLPDAMDFVTAAVLGCRFATAFHALTAQA
                     RLRTEEWLAVFGCGGVGLSAVMIAVALRARVVAVDPSPAALARAASFGAEVTVAATDP
                     VPTVRSITGG"
     gene            843..3050
                     /locus_tag="VFC01_13400"
     CDS             843..3050
                     /locus_tag="VFC01_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019666885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZN80662.1"
                     /translation="MPAPASQDAVPPDAMAAAGPLSRRRFLTYVVAAPVLTVAASSAG
                     GLVAPGKAFAVVPSPPQPENILDLGDVMVAATKQAKRLLVLEVTTENRVVFRLPRAEV
                     GQGVTTALAMVVADEIDARLSDVDVPLEDARQDLGTAQSTGGSSSVRSLWDPVRSVAA
                     EARARLVTAAAERWDLPARDLTTRDTAVWAPDGRSATYGSLTEAAAKVSVPVVSADPK
                     SSSEYRLIGKATTRIDARSIVTGTAKYALDVDVPGALPTVVVRPPTLKGKVKSYDASA
                     ALKMPGVVAVTELPSGVAVSAKTFDQALKARSAVQVTWGSGTVDGVSDADIKARLAAA
                     VPPMTPAPPLTKRVDATFSFAFVNHAPMEVGSAVADVQSGSAEVWVASKSPTGAQSAV
                     ADAIGLSAEDVTLHVIRGGGSFGRRIYYEPAVEAARVSKAIGKPVKLMWTRNDDMRHG
                     RVRPRSQHQLRAVYAGGEVLSYEHRASCVELDLGSGTGQALLDAGYVNPTIGAAFFNL
                     SQSCPYNFGVVTESLNEVSYDMPTAAWRSVYSAQARTAEEILVDEIAKALGTDPVALR
                     RKFLKTADARAVLDKVATEGNWGRSMSSGTAQGVALHGEYRSIAACLVEINCTGDKPR
                     VTKAVMALDVGRQVNPSGLKAQAMGSLIDGISTVLLVGNHLDDGAFREGSYSDFKYAR
                     QADAPLTCDVHLLGGSGKPGGVGELCVPAAAGAVANAYARATGDKPRTFPINF"
     gene            3061..3516
                     /locus_tag="VFC01_13405"
     CDS             3061..3516
                     /locus_tag="VFC01_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN80663.1"
                     /translation="MPSYAFKLNGKSVKVDAPADMPLLWVLRDLLGVTGPKYGCGVGV
                     CRACTCHVNGSAVQVCTLTAADVVGKSVTTIEGLAPQGGLHPVQQAWLDEDVAQCGFC
                     QPGQIMQAAALLKAKRTPSDSDIDQIVNVCRCGTYCRIRKAIKRAASTM"
     gene            3726..4331
                     /locus_tag="VFC01_13410"
     CDS             3726..4331
                     /locus_tag="VFC01_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN80664.1"
                     /translation="MSTEDPPAASNSLMESQGAAAAAVEPPAATAAVEPAVEALNESL
                     SFDDFYRREHRHVLGLAFVLTGNQWVAEDTAQEAFTAAFRRWRSIVAYDSPGAWVRRV
                     TCNRAASVLRRRVREAKALMRLAGRTQTYIELDEGDEAFWQAVRCLPPRQAQAVALYY
                     LEDYSVREISEVLDCSEGTVKTHLSRAREAVARQLRLEDAQ"
     gene            4328..6394
                     /locus_tag="VFC01_13415"
     CDS             4328..6394
                     /locus_tag="VFC01_13415"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HZN80665.1"
                     /translation="MSDFDSRAHKAADSVRQQIAANPLHDEQGIRRAQRSRARAVIPS
                     AVAAAAVLIVGVAVALPRGGDPGTPSGGGATAFAMAGALKPFDTCDTVLQYFKDQAPE
                     YLIERARGGVVTSTDGAGAAERTTAGPKQGRAADSSGTTVESSGAPPEHSTTNVQEAG
                     VDEPDIVKTDGNRIVAVAQARVHLVGLDAGKMTLRKVLPDTMVRNVFLSGERLLVFSA
                     QTAQNFEPGLAWAGQQAVLSMYDVSNLSDPKRIATLTIDGDVLDARLVGTQVRVVTAS
                     SPDIDAPSPVFTPDGGIAEKSKDELRAAVANTSVDDWIPTYTLQDGMGAEMSKGPLVE
                     CANLARPETFSGLDTVAVSSFDMGSALQSRKAVGVIAGGQQIYATDKSTYVSTTDWSA
                     DGSPAKTSLHKFVTAPSGTSTYKGSGEVPGTLLNQYAMSEYDGVLRVASTVSERRGWT
                     NSRQITEGLVTTFGEQGGALRQLGQVGGLGRQDNESIRAVRFIEERGYVVTFRQTDPL
                     YVLDLHDAAAPKVVGELKIPGYSGYLHPIGENLLLGVGQSGLEPGTSPAPNRGSQIGV
                     QFSLFDISDPASPRRIDTQTYGGGAAAAEFDPKAFLYWQPHDLIIAPTNLHGDYRGRG
                     AFSGLVLLRANADGLKELGRLGTTEAYGSVARSLVIGDTVYMLSDHALQANSLDTHRE
                     IDKLIF"
     gene            complement(6480..6899)
                     /locus_tag="VFC01_13420"
     CDS             complement(6480..6899)
                     /locus_tag="VFC01_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HZN80666.1"
                     /translation="MTDTERHTRDAEIEAITRVVHILEHAQQNELPDEFVGLFRQDAI
                     WTTGGGKRLIGRDEISAFTHQVLPGAMKDSMATYEVAHVLFVRPDVAAVKVRQRPVTL
                     AGKPIEGQSEGSPLYVMAKEGGQWRIVAGQNTVVLDS"
     gene            7060..7470
                     /locus_tag="VFC01_13425"
     CDS             7060..7470
                     /locus_tag="VFC01_13425"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN80667.1"
                     /translation="MNRADAQAWLDSYVAAWLSYDEEAIRALFAEDVAYRYHPFDDPI
                     VGREAVVASWLGESDSDDASTRDTPGTYSADYAPVAVDEDVVVATGTSRYSEQPNGPI
                     VRSYHNCFVMRFDNEGRCREFTEYYNKQPEAQSD"
     gene            complement(7756..>8221)
                     /locus_tag="VFC01_13430"
     CDS             complement(7756..>8221)
                     /locus_tag="VFC01_13430"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HZN80668.1"
                     /translation="EPEIAPAEPTQVRTGVATPDETDKSEDLNGDEINIDDSVDLRVG
                     DCFDLKDPLAEIEHIKKVPCTTEHDYELFYVGAMGKRSHPTLDAFVDYVIEYCDPAFG
                     HYIGKAVDDSDLDYDWLVPTEDAWRSGDRTVQCAGYDPNDPLLTRSLRGIRR"
BASE COUNT         1523 a         2594 c         2634 g         1470 t
ORIGIN      
        1 cgcctccggt gatcgaacgc accgtcggca ccgggtcggt cgcggccacc gtgacctcgg
       61 cgccaaagct ggcggcgcgc gccaatgcgg ccggcgaagg gtcgacggcc acgacccgag
      121 cacgaagtgc gaccgcgatc atgaccgcag agagtccgac accaccgcag ccgaacaccg
      181 ctagccactc ctccgtcctg agccgcgcct gtgcggtgag cgcgtggaaa gcggtggcga
      241 agcggcagcc caaaaccgcg gcggtgacga aatccatcgc gtcaggaagg cggaccagat
      301 tgaaatccgc ggcgggaatt gcgacctgct cggcgaagga gccggcctga ctgaagccgg
      361 gctgagtctg atgagggcag acctgcgctt gccccgccat acaccagtca cattgcccac
      421 acccattgac gaacggcaca gtgacccgat ccccgactgc gaacgccgaa acttgcgaac
      481 ccgtcttggt aacaactccg gagaactcgt gaccgggcac gtgcggcagc gaaaccacct
      541 catggcctcg ccaggcgtgc cagtcagatc gacagattcc cgtcgccttc acatcgatca
      601 cggcaccgtc ggctggacat tctgggggtg cgatttcggt aacacggagc ggtgaaccaa
      661 cggcatcgaa gacaacagca cgcattcacc cggttttacc agccagcaca tggttacgaa
      721 gattgcctgc agggtcgaag tgagttggct acgctgccgc gtgggcagcg caccagtcga
      781 gcgaggggtc gcgcagacgc ctgacgcggc ccggacacca accccgcagc gaaggacgct
      841 tcatgccagc ccccgcctcg caggatgccg tgccgccgga tgccatggcc gccgctgggc
      901 cgctgtctcg acgccggttc ctcacctacg tcgtggcagc tcccgtactc accgttgccg
      961 ccagcagcgc tggtggtctg gtagccccgg gcaaagcgtt cgccgtcgtg ccgagcccgc
     1021 cgcagcccga gaacattctc gacttgggcg atgtcatggt ggcggcaacg aagcaggcta
     1081 agcgtcttct ggtgctcgag gtgacgacgg agaaccgtgt cgtcttccgg ctgccacgcg
     1141 ctgaggtcgg acagggtgtt acgacagcac tcgccatggt ggtcgccgac gagatcgacg
     1201 cacgcctcag tgacgttgac gttccattgg aggacgctcg ccaggatctc ggaactgcgc
     1261 agtccaccgg cgggtcgtcc tcggtgcgct cactgtggga cccagtgcgc tcggtggctg
     1321 ccgaggcccg cgcccgtctg gtcaccgcgg cggcggagcg gtgggacctg ccggcacgtg
     1381 atttgaccac gcgggacacc gctgtctggg ccccggatgg tcgctccgcc acatacggat
     1441 cactcaccga agcggccgcc aaagtatccg taccagtggt ctctgctgat ccgaagtcct
     1501 catcggaata ccgcctcatc ggcaaagcga cgacccggat cgacgcgcgg agcatcgtga
     1561 cggggaccgc gaagtacgcc ctcgatgtcg atgtgccagg cgccttgccg acagtcgtgg
     1621 tcagaccgcc cacgctcaaa ggcaaagtca agtcctacga cgcatcggcg gctctcaaga
     1681 tgcccggggt cgtggccgtg accgagcttc cgagcggggt ggcagtgagc gcgaaaacct
     1741 tcgaccaggc attgaaggca cggtcggcgg ttcaggtcac ctggggctcc ggcacagttg
     1801 atggggtctc cgacgccgac atcaaagccc ggctcgccgc cgcggttccg ccgatgacgc
     1861 cagcgcctcc gctgaccaaa cgggtggacg ccactttctc attcgcattc gtcaaccacg
     1921 ccccgatgga ggtcggcagc gcggtcgccg acgtccaatc tggatccgca gaagtctggg
     1981 tggcgagcaa gtcgccgacc ggcgctcagt cggcagtcgc cgacgctatc gggctgagcg
     2041 ccgaggatgt gaccctgcac gtgatccggg gcggtggctc cttcggccga cgcatctact
     2101 acgagccggc agtcgaggcg gcgcgcgtct cgaaggcgat cggaaagccc gtgaagctca
     2161 tgtggacccg caacgatgac atgcgccatg gacgggtacg gccccgcagc cagcaccagt
     2221 tgcgtgccgt gtacgccggc ggtgaggtgc tgtcgtacga gcaccgcgcg tcgtgcgtgg
     2281 agctggatct gggcagcggc accgggcagg cgctcctcga tgcgggctac gtcaacccga
     2341 caatcggtgc ggcgtttttc aacctgagcc agtcatgccc gtacaacttc ggcgtcgtca
     2401 ccgaaagcct caacgaggtc tcctacgaca tgccaaccgc tgcctggcgg tcggtctact
     2461 ccgctcaggc tcgtaccgct gaggagatcc tcgtggacga gatcgcgaag gccctgggaa
     2521 ctgacccggt cgctctgcgc cggaagtttc tcaagaccgc cgatgctcgc gccgtactcg
     2581 acaaggttgc taccgaaggc aactggggca ggtcaatgtc gtccgggacc gctcagggcg
     2641 tcgcgctgca tggggagtat cggtcgattg cggcatgcct ggtcgagatc aactgcactg
     2701 gcgacaagcc tcgagtcacc aaagcggtca tggccctgga tgtggggcgt caggtgaatc
     2761 cgagcggtct gaaggctcag gccatgggca gcctgatcga cgggatcagc actgtgctgc
     2821 tggtcggcaa tcacctggat gacggtgcgt ttcgggaggg cagctacagc gatttcaagt
     2881 acgcccgcca ggccgacgcg ccactgacgt gcgatgtgca cctactcggt ggaagcggta
     2941 agcccggtgg tgtcggcgaa ctgtgcgttc cggcggcagc cggcgcggtg gccaatgcct
     3001 acgcccgcgc cactggcgac aagccccgta ccttcccgat caacttctga ggagcacccg
     3061 gtgcccagct atgcattcaa gctcaacggc aagagcgtca aggtcgatgc gccggccgac
     3121 atgcccctcc tctgggtgct gcgcgacctg ctcggcgtga ccgggccgaa gtacggatgt
     3181 ggcgttggcg tatgccgcgc gtgcacctgc cacgtcaacg gcagtgcggt tcaggtctgc
     3241 acgctcacag cggccgacgt ggtgggaaag tcagtcacca cgatcgaagg tctggcccct
     3301 caaggtggct tgcacccggt acagcaggcg tggctcgacg aggacgtggc gcagtgcggc
     3361 ttctgccagc ccgggcagat catgcaggct gcggcgctgc tgaaggccaa gcggaccccc
     3421 tctgattccg acatcgacca gatcgtgaac gtgtgccggt gcggcacgta ttgccggatc
     3481 cgaaaggcga tcaaacgcgc cgcgtccacg atgtgaagcc gacattcgag cgctcgggta
     3541 cttcccactt cagtcagcct cggctcggga accccctcca cggacggacg ttgtggtact
     3601 tgtgaagccg cactgccttc atctgcactt ttctcctggt tttggtcgcc aggtctgcgc
     3661 catcgcccga cgatctggcg caatgctcgg cgcggaatga agtttccgga gaaaatctct
     3721 ccgtcatgtc aaccgaagac cctcccgcgg cgagtaactc attgatggaa agccaaggag
     3781 cagcggcagc cgccgtcgaa cccccagcag cgacggccgc cgtcgaacct gcagtcgagg
     3841 cgctcaacga gtcgctgtcg ttcgacgact tctacaggag agaacaccgc catgtgcttg
     3901 gtctggcgtt tgtactgacc ggcaaccaat gggtggccga ggacaccgcg caagaggcgt
     3961 tcacggctgc attccgacgc tggcgttcca tcgtggccta cgactcgccg ggcgcatggg
     4021 ttcggcgagt cacctgcaat cgggcagcgt cggtattacg acgacgcgta cgcgaggcaa
     4081 aggcgttgat gcgtcttgcc gggcgaacgc agacctacat cgaactggac gaaggcgatg
     4141 aagcgttctg gcaagcggtt cgttgtctgc caccccgcca ggcccaagcg gtagccctct
     4201 attacctgga ggactacagc gtgagggaga tctccgaggt ccttgactgc agcgaaggca
     4261 ccgtcaaaac ccacctgagc cgggcgcgcg aggcggtcgc gcgtcagctg cggttagagg
     4321 atgcacaatg agcgacttcg attcacgagc acacaaagca gccgattcgg ttcggcagca
     4381 gattgcggcg aaccctctcc acgacgagca gggaatccgg cgcgcgcaac gatcgcgtgc
     4441 ccgcgcagtc atcccgagcg ctgttgccgc cgcagcagta ctcatcgtcg gagtcgcagt
     4501 cgcattaccc cgcggcggcg atcccggaac accgtcgggt ggaggggcga ccgcgttcgc
     4561 gatggcagga gcgttgaagc cgttcgatac ctgtgacacc gtgctgcagt acttcaagga
     4621 tcaggctcct gagtatctga tcgagcgcgc ccgcggtggc gttgttactt cgaccgacgg
     4681 ggccggtgca gcggagcgga ccactgcggg acctaagcaa ggccgtgccg ctgactcatc
     4741 gggtacgact gttgagtcgt caggtgcgcc gccggagcac tcgacaacga acgtgcaaga
     4801 agcaggcgtt gatgaacctg acatcgtcaa gacggatggc aatcgcattg ttgcggtcgc
     4861 tcaggctcgg gtgcatttag ttggcctcga cgccggcaag atgacattac gcaaggtcct
     4921 tccggacacc atggttcgca acgtatttct gtccggcgag cgtctgcttg tgttcagcgc
     4981 tcagacggct cagaacttcg agcctggact ggcctgggca ggtcagcagg ccgtcctgtc
     5041 gatgtatgac gtctcgaatc tcagcgatcc aaaacgaatt gccacattga cgattgacgg
     5101 cgatgtgctc gatgcacgac ttgtgggaac ccaggtgcgt gttgtgacgg cttcgtcgcc
     5161 cgacatcgat gctccatcgc cggtcttcac gcccgatggc ggtatcgctg agaagtccaa
     5221 agacgaatta cgggctgcag tcgcgaacac gagtgtcgac gactggattc cgacgtacac
     5281 actgcaagac ggtatgggcg cggaaatgag caagggacct cttgtggaat gcgccaatct
     5341 ggctcgtccc gaaaccttct ccgggctcga cacggtggcg gtgtcgtcgt tcgatatggg
     5401 gtcggctctg cagtcccgca aggcggtggg cgtgatcgcc ggcggacagc agatctatgc
     5461 gaccgacaag tcgacgtacg tctcgacgac tgattggagt gccgacggtt cacctgcaaa
     5521 gacgagcttg cacaagttcg tcaccgcgcc atcggggacg agtacctaca aaggatcggg
     5581 tgaggttccc ggaaccttgc tgaatcagta cgcgatgtct gaatacgacg gagtcttacg
     5641 tgttgcaagc acggtttctg aacgccgggg ctggaccaat tctcgtcaga taaccgaggg
     5701 tctggtcacg actttcggtg agcagggtgg tgcgcttcgc caactgggac aggtcggcgg
     5761 gctcggtagg caggacaacg aatcgattcg agcggtgcga ttcatcgaag agcgcgggta
     5821 tgtcgtgacg ttccgccaaa ctgatcccct ttacgttctc gaccttcacg atgccgccgc
     5881 cccgaaggtc gtgggcgagc tgaagatccc cggctactcc ggctatctgc atccgattgg
     5941 ggagaacctg ttgctcggtg tcgggcaaag cgggttggaa ccgggcacca gccctgcgcc
     6001 gaatcgtggc agccagatcg gcgtgcagtt ctccttgttc gacatcagcg atcccgcgtc
     6061 tccgcgccgt atcgacacgc agacctacgg tggtggtgct gcggcagccg agttcgaccc
     6121 gaaggcgttc ctgtactggc agccgcacga cttgatcatc gctccaacga acctgcatgg
     6181 tgactaccga ggacggggag cgttcagcgg tctcgtgctg ctgcgagcca acgcggatgg
     6241 actcaaggag ctcggccgcc ttggaaccac cgaggcctac ggaagcgtcg cccgttctct
     6301 cgtcattggt gacaccgttt acatgctttc ggaccacgct ctgcaggcca acagtctcga
     6361 cactcaccgc gagatcgaca agctgatctt ctaacgaggt gatcgtgatg cgattgctgc
     6421 tggacggacc ggcgcggccg actgaatgca gacgccgcca cggctaggag cgccagcctt
     6481 cacgagtcga gcacgaccgt gttctggcca gcgacgatgc gccactgccc gccctccttg
     6541 gccatgacgt agagcgggct gccctcgctc tggccctcga ttggcttacc cgcgagcgtc
     6601 accggccgct ggcgtacctt gactgcggcg acgtcgggtc gaacgaagag cacgtgcgca
     6661 acctcgtacg ttgccatcga gtccttcatc gctccgggga gtacctggtg ggtgaacgcc
     6721 gagatctcgt ccctcccgat gaggcgcttt ccgccgcccg tcgtccagat cgcgtcctgt
     6781 cggaacagac cgacgaactc gtcagggagc tcgttttgtt gtgcatgctc gagtatgtgg
     6841 accactcgcg tgatcgcctc gatctccgcg tcgcgggtgt gccgttcggt gtccgtcata
     6901 ttcacagcac cagggtgtta cctgtagtca acttgaggtc aactgacttc ccgactgatg
     6961 ccgccacagc cagtcaggct attcgcgacg tcgtcgggtc ggtgcggacc tccagaccac
     7021 tcaactagcg gacgggccgt tcgaattagg gtggtgggcg tgaaccgtgc tgacgcgcag
     7081 gcctggctcg atagctacgt cgcggcgtgg ctgtcctacg acgaagaagc catcagggcg
     7141 cttttcgccg aggacgtcgc ctaccgatac cacccctttg acgatccaat cgtcggccga
     7201 gaggcggtcg tcgcatcgtg gctgggcgag tctgactcag acgatgcctc gacccgcgat
     7261 acgcccggca cgtactcagc ggattacgca ccggtcgccg tcgacgagga cgtcgtggtc
     7321 gcgacgggca cctccaggta cagcgagcag cccaacggtc ccattgttcg cagctaccac
     7381 aactgtttcg tgatgcgttt tgacaacgaa gggcgctgcc gcgagttcac cgagtactac
     7441 aacaagcagc cagaagcaca gtctgactag gggagatgct tgacgacagc cccggatggc
     7501 cgcgaaatga ccgcaggaat ctgattcctg ctcgtcatca cactgcctat ggcctaccga
     7561 gtctcttcat ccgagtcggg aaataagtgg ccgtacgcat ccagcgtggt gttgtcccag
     7621 acccgcgaca gtgctgtcga tctcagcagt ggcggcctgg ttgccgccac gggtgtcgac
     7681 ccgcgcagat gatcgcatgt gaatcgagga ttaccttcga gcccgcgcag aatcttagag
     7741 gtccgaccgc agggcttagc gccgaatgcc ccggagcgac cgtgtcagga gagggtcatt
     7801 cggatcgtag ccggcgcact gcacggtccg atcgcccgat cgccaggcat cctcggtcgg
     7861 gaccagccag tcgtagtcga ggtcggaatc gtcgacggcc ttgccgatat aatgaccgaa
     7921 cgcggggtcg cagtactcga tcacgtagtc cacgaacgcg tcgagggtcg ggtggctgcg
     7981 cttgcccatc gccccgacgt agaagagctc gtaatcgtgc tctgtcgtgc acgggacctt
     8041 cttgatgtgc tcgatctcgg cgagcggatc cttgagatcg aaacagtccc cgacgcgcag
     8101 gtcgacgctg tcgtcgatgt tgatctcatc cccgttgagg tcttccgact tgtcggtctc
     8161 gtcgggcgtc gcgaccccag tgcggacctg agtcggctca gcaggtgcga tctcgggctc
     8221 a
//
LOCUS       DASRJA010000310         5254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1163006,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000310 DASRJA010000000
VERSION     DASRJA010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163006"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VFC01_13435"
     CDS             complement(<1..834)
                     /locus_tag="VFC01_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN80669.1"
                     /translation="MTPLDGQATAIPTVTLNDENTIPVIGLGVGELSDSETEQAVSTA
                     LEAGYRLIDTAAAYGNEEAVGRAIASSSVPRDELYVTTKLATPDQGFQASQDACKASV
                     ERLGLEYVDLYLIHWPGGDHGKYVDSWGGLMKCKEDGDTRSIGVANFHAEHLANIIDL
                     SFFTPAINQIELHPLLNQAELRSINAGYGIVTEAYGPLGVGKLLDNPTIATVAQAHGK
                     TPAQVLIRWSIQLGNVVIPRSSSPERIKSNLEVFDFELTDDQMATLNGLDDGTRFRPN
                     PD"
     gene            complement(840..1670)
                     /locus_tag="VFC01_13440"
     CDS             complement(840..1670)
                     /locus_tag="VFC01_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN80670.1"
                     /translation="MAAPLITLNDGNAMPQVGLGVWQTPPEDTERAVTTALDAGYRHI
                     DTAAGYFNEREVGKSVATSGLPREDVFVTTKLWNADQGYDKTLAAFDASMDRLGLQYL
                     DLYLIHWPMPARNAFVDTFKAFAHLRDQGRIRSIGVSNFEPEHLRILVDATGIVPAVN
                     QIELHPLLQQQELRDVHAQQGIVTEAWSPLGQGSLLSNPTVTTVADAHGKTPAQALIR
                     WHIQLGNIVIPKSVTRERIVSNFDVFDFELSEQDMASISSLGDGTRLGPDPRTFNFTG
                     "
     gene            1669..2766
                     /locus_tag="VFC01_13445"
     CDS             1669..2766
                     /locus_tag="VFC01_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN80671.1"
                     /translation="MTQSLPAHASWSPSRRDALINTAAGITLRALPRIPDAVKRLMLG
                     GRSITIDGNTLDTTLQLMLAGQRATGIDGLAAEDDVIGSRARLEILAASFKQDILVDR
                     VTNLSIPGPVGPIPARHYQPVNGEGEPLLVFYHGGGQVIGSLDTHDDLCRKICRDGAM
                     HVLSVDYRLAPEHKAPAGADDAFAAFVWARDHAADLGADADRVAVGGDSAGGNLAALV
                     AQRARNEGPGLPALQLLIYPVTNYRDETRSQTLFAKGYFLTKHDLSWFRHNYLDGAQV
                     DASDPRVSPLLADDLSGLPPAMVLTGGFDPLRDEGRQYVDAVRAAGVTVDHRQFGSLV
                     HGFANFFPLGGGSAAATTEMISALRAHLARA"
     gene            2801..3577
                     /locus_tag="VFC01_13450"
     CDS             2801..3577
                     /locus_tag="VFC01_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZN80672.1"
                     /translation="MATKPKRYDLKAADRKRSLAIQIGLTSIVVIFAVVLVGYIVLSN
                     DKKPTSGEVKSIRVASSNVIKNEGSSDPKVVLSLYEDFLCPHCGLFEQQFGPTVSKLI
                     DTGAVAADYYMVAILDKQGNGYSSRAANAAYCVADESTDAFRRFHAALYAQQPAEGSA
                     VYPDNARLIEIARQAGAGGGVPDCINKGRYTEMAQGLAEASKIQATPTIRINGQDYET
                     STPDALVAKIKEIVGNVPGLDGAAPPAQAPAPAQAPAPAP"
     gene            3574..4206
                     /locus_tag="VFC01_13455"
     CDS             3574..4206
                     /locus_tag="VFC01_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HZN80673.1"
                     /translation="MTVTAPSDVQPSEPAADASNRVPVRTASAVWILIAGVVGLAAAL
                     TLTVEKIEILINPDYVPSCSINPVLSCGSVMVTPQASAFGFPNPLIGIVSFTIVVVTG
                     VLALTKVRLPRWYWAGLATGTLLGVAFIHWLIFQSLYRIGALCPYCMAVWAVTIPLFV
                     VVASIALQPLQSNVVARVLYTWRWSLVVLWFTALVLMILVRFWDYWSTLI"
     gene            4238..>5254
                     /locus_tag="VFC01_13460"
     CDS             4238..>5254
                     /locus_tag="VFC01_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HZN80674.1"
                     /translation="MISKVLVANRGEIAIRAFRAAYEMGIATVAVYPYEDRNSLHRLK
                     ADESYQIGETGHPVRAYLSVDEIIRVAQQAGADAIYPGYGFLSENPDLAAACAEAGIT
                     FVGPSAEILELAGNKSRAIAAARAAGLPVLNSSAPSSSPDELMAAAATMEFPLFVKAV
                     SGGGGRGMRRVSDLAGLPEAIEAASREAESAFGDPTLYLEQAVLNPRHIEVQILADTH
                     GHVMHLFERDCSVQRRHQKVIELAPAPNLPDELRQRICADAVAFARQINYSYAGTVEF
                     LLDERGHYVFIEMNPRIQVEHTVTEEITDVDLVASQLRIADGETLEDLGLSQDTLTVR
                     GASMQ"
BASE COUNT          898 a         1725 c         1705 g          926 t
ORIGIN      
        1 gtcgggattc gggcggaacc gcgtgccgtc gtcgagcccg ttgagcgtgg ccatctgatc
       61 gtcggttaac tcgaagtcga acacctcgag gttcgacttg atccgctccg gtgacgacga
      121 gcgcggaatc acgacgttgc cgagctgaat gctccaccgg atcagcacct gcgccggggt
      181 cttgccgtgc gcctgcgcca ccgtggcgat cgtcgggttg tcgagcagct tgccgacacc
      241 aagtgggccg taggcctcgg tgacaatgcc gtacccggcg ttgatcgacc gcagctcggc
      301 ctggttcagc aacgggtgca gctcgatctg attgatcgcg ggggtgaaga acgagagatc
      361 gatgatgttc gccaggtgct cggcatgaaa gttggccaca ccgatggacc gggtgtcgcc
      421 gtcttccttg cacttcatca ggccgcccca gctgtccacg tacttgccgt ggtccccacc
      481 cggccagtgg atgaggtaga ggtccacata ctccaggccg agccgctcca cgctggcctt
      541 gcaggcgtcc tgtgatgctt gaaagccctg gtcgggggtg gccaatttgg tcgtgacgta
      601 cagctcgtcg cgcggcacgc tcgacgaagc gatagcccgg ccgaccgctt cctcgttgcc
      661 gtaggcggcc gcggtgtcga tcagtcggta tcccgcttcc agggccgtgg acaccgcctg
      721 ttcggtttcg gagtccgaca gctcgccgac gccaagacca atcaccggga tcgtgttctc
      781 gtcgttgaga gtgacggtgg gaatcgcagt cgcctgcccg tccagtgggg tcatggcacc
      841 tatcctgtga agttgaatgt tcgtgggtcg ggaccaagtc gtgttccgtc tccgagcgaa
      901 gagatggacg ccatgtcctg ctcgctcagc tcgaaatcga acacgtcgaa gttactcaca
      961 attctctcgc gggtcaccga cttcgggatg acgatattac cgagttggat atgccaccta
     1021 atcagtgcct gggctggagt ttttccgtgg gcgtcggcaa cggtggtgac cgttggattg
     1081 ctcagcagtg agccctgccc gaggggactc cacgcctcgg tcacgatgcc ctgctgagcg
     1141 tgcacgtccc gcaactcctg ctgctgcagc agcggatgca actcgatctg gttaacggcg
     1201 gggacaattc ccgtggcgtc caccaggatt cgcaagtgtt cgggttcgaa gttgcttacc
     1261 ccgatcgagc ggatcctgcc ttggtcgcgt aggtgcgcga acgctttgaa cgtgtcgacg
     1321 aacgcattgc gcgccggcat cggccagtga attaggtaga ggtcgaggta ttgcaggccg
     1381 aggcggtcca tgctggcgtc gaacgccgcg agcgtcttgt cgtagccctg gtcggcgttc
     1441 cacaacttgg tggtgacgaa cacgtcctcg cggggcagac cagatgtcgc gacagactta
     1501 cccacctcgc gttcattgaa gtagcctgcc gccgtatcga tgtgccgata accggcgtcg
     1561 agcgcggttg tcaccgcacg ctcggtgtcc tccggcggcg tctgccaaac gccaagtccg
     1621 acctgaggca tcgcattacc gtcattcagc gtgatcaagg gagcagccat gacccagagt
     1681 ctgccagcac acgcgtcgtg gagtccgagc cgcagggatg cgctcatcaa caccgcggcc
     1741 ggcatcacgc tgcgcgcact gccgcgcatt cccgacgccg tgaaacgcct gatgcttggc
     1801 ggtcgttcga tcaccatcga cggcaacacg ctggacacca cgctgcaatt gatgctggcc
     1861 gggcagcggg cgaccggcat cgacgggctg gcggcagaag acgacgtgat cggctcacgg
     1921 gcccgactgg aaatcctggc ggcgagcttc aagcaggaca tcctggtgga cagggtgacc
     1981 aatctctcga tccccgggcc cgtcggcccc atcccggccc ggcactacca gccggtcaac
     2041 ggcgagggcg aaccgctgct ggtcttctac cacggcggcg gccaggtgat cggcagcctc
     2101 gacacccacg acgatctgtg ccggaagatc tgccgcgacg gtgcaatgca cgtgctgtcg
     2161 gtcgactacc ggttggcgcc ggagcacaag gcacccgccg gcgccgacga tgccttcgcg
     2221 gcgttcgtct gggcgcgcga ccacgccgcc gacctcgggg ccgacgcgga ccgagtggcc
     2281 gtcggcggcg acagtgcggg cggcaacctc gccgcgctgg tagcgcagcg ggcgcggaat
     2341 gaggggccag ggctgccggc actgcaactg ctgatctatc ccgtcaccaa ctatcgcgac
     2401 gagacccggt cgcagacgtt gttcgccaag gggtacttcc tcaccaagca cgatctgagc
     2461 tggttccggc acaattacct cgacggcgcg caggtcgacg cgtccgatcc gcgggtgtcg
     2521 ccgctgctcg ccgacgacct atccgggctg ccgcccgcga tggtgctcac cggtgggttc
     2581 gatccgctgc gcgacgaagg ccgccaatac gtcgatgctg tgcgggccgc aggagtcacc
     2641 gtcgaccatc gccagttcgg atcgcttgtg cacgggttcg cgaacttctt cccgctcggc
     2701 ggcggcagcg cagcggccac gacggaaatg atctccgcgc tgcgcgccca tctcgcccgc
     2761 gcatgaaaag tctccgctga cccgccggta ctcttgaccc gtggcgacca aacccaagcg
     2821 atacgacctg aaggcagccg accgcaagcg cagcctggcg atccagatcg ggctgacctc
     2881 tatcgtggtg attttcgccg tcgtgctggt gggttacatc gtcttgtcca acgacaagaa
     2941 acccacatca ggagaggtca agtcgatccg cgtcgcgtcg agcaacgtca tcaagaacga
     3001 aggcagctcc gacccgaagg tcgtgctgtc cctctacgag gacttcctgt gcccgcactg
     3061 cggcctgttc gagcagcagt tcgggcccac cgtgagcaag ctcatcgaca cgggcgccgt
     3121 cgccgccgac tactacatgg tcgccatcct cgataagcag ggcaacggat actcgtcgcg
     3181 ggccgcaaac gccgcatact gcgtggccga cgaatccacc gacgcgttcc ggcgcttcca
     3241 tgccgcgctc tacgcacagc agcccgcaga aggctccgct gtctaccccg acaatgcgcg
     3301 gctgatcgag atcgcgcgcc aggcgggcgc tggcggtggc gtaccggact gcatcaacaa
     3361 gggccggtac accgagatgg cgcagggact ggccgaagcg agcaagattc aggccacgcc
     3421 gaccatccgc atcaacggcc aggactacga aaccagcacg cctgacgcgt tggtcgccaa
     3481 gatcaaggaa atcgtcggca acgttccggg gctcgacggc gccgcacctc cggctcaggc
     3541 acccgcgccg gcacaggcac cggcaccggc gccatgaccg tgaccgcgcc cagcgacgtc
     3601 cagccgagcg aaccggcggc tgacgcgtcg aaccgggtgc cggtccggac agcgagcgcg
     3661 gtatggatcc tgatcgccgg cgtcgtcggc cttgccgcgg cgctgacgct gacggtcgag
     3721 aagatcgaga ttctgatcaa ccccgactac gtgccgtcat gcagcatcaa cccggtgctg
     3781 tcgtgcggtt cggtgatggt gacgccgcag gcgtcggcgt tcggcttccc caacccgctg
     3841 atcggcatcg tctcgttcac catcgtggtg gtaacgggcg tgctcgcgct caccaaggtc
     3901 aggctgccgc ggtggtactg ggccggcctg gccaccggaa cgctgctggg cgtggcgttc
     3961 atccactggc tgatcttcca aagcctgtac cggatcggcg cgctctgccc gtactgcatg
     4021 gcggtgtggg cggtgaccat cccgctgttc gtggtggtcg cctccatcgc attgcagccg
     4081 ttgcagtcga acgtggtcgc tcgggttctc tacacgtggc ggtggtcgct ggtggtgctg
     4141 tggttcacgg cgctggtcct gatgatcctg gtgcgcttct gggactactg gtccacgctc
     4201 atataactac ggcgtcatcg ggggataggg tcagcgggtg atttcgaagg ttctggtcgc
     4261 caaccgcggg gagatcgcga tccgcgcctt ccgggcggcc tacgagatgg gcatcgcgac
     4321 ggtcgccgtc tatccgtatg aggaccgcaa ctccctgcat cgtctgaagg ccgatgagtc
     4381 gtatcagatc ggcgagacgg gccaccccgt ccgggcctac ctttcggtcg acgaaatcat
     4441 tcgggtagcc cagcaagccg gcgccgacgc catctatccg ggttacggat ttctgtcgga
     4501 aaaccctgac ctggccgcag cctgcgccga ggcgggcatc acgtttgtcg gtccgagcgc
     4561 ggaaatcctc gagctggcgg gcaataagtc gcgggcgatc gcggcggcac gcgccgccgg
     4621 gttgccggtg ctgaactcct cggcgccgtc gtcgtcaccc gatgagctga tggccgccgc
     4681 cgcgacgatg gagtttccgt tgttcgtcaa ggcggtatcc ggtggcggcg gacgcggcat
     4741 gcggcgcgtc agcgacctcg ccgggctgcc cgaggcgatc gaggccgcgt cccgcgaggc
     4801 agagtccgca ttcggtgacc cgacgctgta cctcgagcag gcggtgctca acccgcgcca
     4861 catcgaggtg cagatcctgg ccgacaccca cggccatgtg atgcacctgt tcgagcgtga
     4921 ctgcagcgtg cagcgtcgtc accaaaaggt catcgaattg gcccctgcgc cgaacctgcc
     4981 cgacgaactg cggcagagga tctgcgccga cgccgtcgca ttcgcccgcc agatcaacta
     5041 cagctatgcc ggcaccgtcg aattcctgct cgacgaacgc ggccactacg tgttcatcga
     5101 gatgaacccg cgtatccagg tcgagcacac cgtcaccgag gagatcaccg acgtcgacct
     5161 ggtcgccagc cagctgcgca tcgccgacgg cgagacgctg gaagaccttg ggctgagcca
     5221 ggacacattg acggtgcgcg gggcctctat gcag
//
LOCUS       DASRJA010000311        16456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1738918,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000311 DASRJA010000000
VERSION     DASRJA010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16456
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738918"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /gene="mmsA"
                     /locus_tag="VFC01_13465"
     CDS             complement(<1..1170)
                     /gene="mmsA"
                     /locus_tag="VFC01_13465"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZN80675.1"
                     /translation="MHNTIPHWINNKPYSGAGTNTSPVTNPATGEVTAQVALATVEDA
                     RVVIDAAAAAFPAWRDTSLAKRTQILFNFRELLNARKGELAEIITSEHGKVVSDALGE
                     VSRGQEVVEFACGIPHLLKGGFTENASTKVDVYSIRQPLGPVGIISPFNFPAMVPMWF
                     FPIAIAAGNTVVLKPSEKDPSASLWLANLWAEAGLPPGVFNVLQGDKTAVDELLTNPK
                     IKSVSFVGSTPIAQYVYFTGTAHGKRVQALGGAKNHAVILPDADLDLAADAMVNAGFG
                     SAGERCMAISACVAVGPIADDLIAKITERTTGLKIGDGTKDSDMGPLVTKAHRDKVAS
                     YIDAGEADGAKIVVDGRQVQADGSAEGFWLGPTLLDNVTPEMSVYTDEIFGPVLSV"
     gene            1251..2864
                     /locus_tag="VFC01_13470"
     CDS             1251..2864
                     /locus_tag="VFC01_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HZN80676.1"
                     /translation="MPLTVAEVVDLPVIQAGQPEVLSARRWDDQIRWVHVSDVADLST
                     LLQGGELVLTTGAALRRSPARYLRGIAEAGALGVIAELPQGVSLPKTAAAAAASANVA
                     LIVLHREIKFVEVTEAVHRRIVSEQFEAVAFDRRVHATFTDLSMKRVSAPGIVAAAAR
                     MLDEPVVLEDLAHQALAVAPGPDTTATLLDDWEYRSRRATAARDGWAMTPVGPRGEEW
                     GRLVVPRHPVDASRAKMVLERAAAALALNRMIERDRSGLHQQAQSGLIDDVIRNRVTD
                     EREITARAHALGLRKTSRYHPVVVRTADASTGSDPVAGQRRNVRLLDAVAHTVNAAGH
                     TGLFTIRGDGEIGAVVALSASRAGAAEKAWSALGLDVAREVARADGGQRSVLALGEPS
                     GLITDAIVGIADAAHVAEAALAMGGEPRPFYRTSDVRLRGLISLLQDDPRLQAFAETE
                     LKALLTAESAGRPTDFTILREYLRLGGNKVALATQLRISRPALYKRLSAIETKLGVDL
                     SDAESTASLHVAMLALEVRRGSDVGVTPS"
     gene            complement(2883..4370)
                     /locus_tag="VFC01_13475"
     CDS             complement(2883..4370)
                     /locus_tag="VFC01_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HZN80677.1"
                     /translation="MVYRVRDLLDTPHLRLTLKGGSDGLDQPVTWAQTSDLEEPWLYL
                     AGGELLMRNGQTLTESARGQTALIRGVAENGMCGMVIGLDAATPALTPAAVALADDLG
                     FPIMTVPYSVGFASIGRAVADAAGSGRVAVTERVYNVIRQSVSNPAPANVLSQLERDL
                     SCRIAVLDAATGAVALEASDPPPEDLREAITDEIGGRRGAIPGVLHVSTRSQRALVVE
                     VPDEEPTALVTYAFRGPTTDMVHLQHLASAVAVLLAQQNARREHERRIGAEVMGQLCD
                     GRMSDRDGDRELAERGLDAASCCVVAAEGVSAASERRLHLGLGRRSIPHLLLRRAALL
                     YALMPVTDHTLDVLNQRLGGEAIFGVSDPLSLPSRAPVAVREATWAVRVAVTTSSQIA
                     RFADATIFPVLRDPEEARVVVDRVLGRLLAYDVAHSSDMVVTLDIYLRHERSLVRTAK
                     ELGIHRQTVVYRMQRIEEITGRRIGDTAAIAEFWLALRARDLLAS"
     gene            4608..6059
                     /locus_tag="VFC01_13480"
     CDS             4608..6059
                     /locus_tag="VFC01_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN80678.1"
                     /translation="MAESIDTAAAAPERLQRGFSFRSALSLAFADVSPIAAVYAIFTL
                     GLFAAGPKFFWAFPIVLIGQLLVAAVFGELASRWPYAGSVYQWSRHVQGTTWGWVAAW
                     AYMWGLTIALGTLSYAAGGFLLNIFGVAEANRWQIASIAIAIILVGTAVNMVGRHVLK
                     VMVAISITCEVVGSLGLGLVLLLFYRENPFSVLFSSAGIPDAASWASGPMLLAIAYVG
                     WSFLGFEAAGSVAEEVDEPERNVPKAIILALLLVGLVVMFSAAALILSIPNLPGVVAA
                     QSGDVVADTLATHLGQGVTKPLLAMFVIGFVSSFLAVQAAVSRCIWGAARDHSLPGSK
                     VLGGLAGPERLPVNAIGLTAVIAIVFVLLAGTQFYNILVNFNIIGFYIAFGVPVIGAA
                     LARLTGKWKPGPFTLGRWGAPITYLASLWIIFETVNVAWPRTQPDQPWYINWASVLTT
                     VVLGVIGVAIYLSVRRGIQAPIAERLGDAPAPQ"
     gene            6056..6739
                     /locus_tag="VFC01_13485"
     CDS             6056..6739
                     /locus_tag="VFC01_13485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN80679.1"
                     /translation="MSSVAIVTGAASGIGASVVAALDEEGYHVVGLDVNDSSTYVVDV
                     SDAGAVDEVMRRVAAEHGPVDAVVSAAGHYESVPIADITRDQLHRMLRVHLGGLRNVV
                     RAVLPSMIERRAGAVVAITSELAVGGGDADAHYAAAKGAIIGLVRSLAAEVAGYGIRV
                     NAVAPGPTDTPLLAADSPWRAPDYLATLPAGRLARPVEIAEVVRYLVGADAAFCTGEV
                     LSPNSGAVI"
     gene            6741..7502
                     /locus_tag="VFC01_13490"
     CDS             6741..7502
                     /locus_tag="VFC01_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80680.1"
                     /translation="MSRHSGRLALVTGATQGIGAALAERLAAEGATVGVNGRVRDARM
                     DSVVTSTGGFPAVADVSDPDAVGALVADIERTRGPVDILVCNAAYMTMAPFVDHADYD
                     WWKIIDTNLAGTVYLVQAVLAGMRRRGAGNIVIIASEWGVIGWPNTTAYSASKAGLIA
                     LTKSLGRELAPDGITVNAIAPGVIDTPQLQVDADDAGISLEQMHEQYGRETPLGRIGK
                     PTEIAAMVSLLARADMGAFVGQTIQINGGTTRCRT"
     gene            7499..8458
                     /gene="speB"
                     /locus_tag="VFC01_13495"
     CDS             7499..8458
                     /gene="speB"
                     /locus_tag="VFC01_13495"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HZN80681.1"
                     /translation="MTEHHQPVEPVDGTVVPRYAGFSTFARLPRIDDVPTYDVAVVGI
                     PFDAGVSYRPGARFGPGHVRQCSRVLRPYNPQMQVSPFANQQVVDAGDIPCTPYDIAT
                     AARQIEEHATALTANGARLITIGGDHTIAYPLLRVHRERYGPVALVHFDAHLDTWDTY
                     FDAPLTHGTPFRRAAEDGLFVSGHSAHVGIRGSHYSPDDLTSDAELGFTVVHCREFQK
                     RSVDDVVGQLRETIGDLPLYVSIDIDVLDPAHAPGTGTPEIGGLTSRELLELVRGFDG
                     LNLVGADIVEVAPAYDHAELTGIAAACLAYEFLALLARARCRA"
     gene            8455..9321
                     /locus_tag="VFC01_13500"
     CDS             8455..9321
                     /locus_tag="VFC01_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HZN80682.1"
                     /translation="MTVPPVPWPNGARCAASFSFDVDAESAMLGVDTSHADRMSAISH
                     QAYGPLSGVPRLLKILAKRGIRSTFFVPGYTAVRYPDVIRSIVEHGHEVAHHGYLHEA
                     MRGLTEAQEAAILDRGLEALESVTGTRPTGYRAPMWELNWHSPKLLRDRGFLYDSSLM
                     DTDHPYELTVDGDSSLVEIPIQWALDDWEQYCYIPEFSGSGLIESPTKAIEMWRLEFD
                     AIRAEGGCFVLTNHPFLSGRPSRAQALDGLIEYVCAQGDVWVASLGEIADHVRGLGLT
                     PRSVERPNPADF"
     gene            complement(9318..10961)
                     /locus_tag="VFC01_13505"
     CDS             complement(9318..10961)
                     /locus_tag="VFC01_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80683.1"
                     /translation="MSDQRYDGPATVAVATTAAPAVARSPRVGYVPARTNIVRDGVAV
                     VLLLLALLLPWDLEVGVGIPGSNGLFLVVVIFVTLLAVAAALAPHVGPFRLAAPQPDV
                     RRTSLMRLLLNVAYLVLALGFVGYHLLQTVRDGGTGLLPPGVGPGLIVGVGGALLAAQ
                     PPITSITIEDNGFRRWYTAARALGAVSIALATLSVAFNLYWRLRYLFVTNGDFGGHDV
                     AVIITTLLYGAVALIALVIASRWLLEKSAAARLATTALGASAVVATTLVWVFPVGRDV
                     DAFHGIAQNTSTAAVGYEGYLFWAAAAAIVAPTTLYAVFLIKPPTVGAYRSAAQKCLT
                     LIAFWAFAAAACRVVDYLIALSLDLPRAAYDSVAMTVFNVITGIIARWLQRQLGRGET
                     TPTVIAAFSGVLFVFTVANLAIGVALAPRYAEPSPDAVYGNNLAQQITSTFDVVICAL
                     SLTVLIAMLFTGPLAGYLVRRRETRVPTAPPAPPSPEPPTAAAPTLGQPAPTSAIPKI
                     VRLKEDSTSVIPAPPTEEFSAPTATLRILRRQPSDPPAD"
     gene            11085..12746
                     /locus_tag="VFC01_13510"
     CDS             11085..12746
                     /locus_tag="VFC01_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80684.1"
                     /translation="MDVVLGVAVTGRVARLAMIGSPASGGQVLDQYALDMPDDAATDL
                     ADTIVGTYRAVADSGNRMAAIRLCMPDASQAETLRQTVLGAGAQNVDVVAETEAAAAL
                     ARSVGADAALLLADDDTVSLTVVGEDEESTSVLASAPIGVAGAAVACAAVLQKVPSAD
                     APVRIMLVGQRLDLDSVAAELRSTAPVEVPPDAGYAIARGAAQTAEESGYPAGAATQM
                     APAAADATMMAPASDATMVAPAAGDATQMAPSTDPAAVGPQLAYSQEEPGDYELPFDS
                     LEEFVPELEDEEAYTAMIAPPPRKTLLMGSALAFVVVSFATLAVTVAINIRPVADVEA
                     QAVPATQAETVPGRFLPQVPHEPDPVALPVAVVSPAPAAPAPSVRTNRGPVDNNVPSA
                     PAPQAPAPEPAPAPGVPVVPIPPVIPLPPLPTLPTFTTTVTTTPTTTTTTPTTTTTTP
                     TTTTTTPTTTSTETTTSTSEPTTIPLSPVTPVIPKTELPATQAPAYTPAPAYTPPPEP
                     AYTPPAVAPVAPAPAPVAPAPAPVIEKPAPAPVIEAPVTTIPVSP"
     gene            12873..13454
                     /locus_tag="VFC01_13515"
     CDS             12873..13454
                     /locus_tag="VFC01_13515"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZN80685.1"
                     /translation="MRSSAPALFPVFRSPSQAEILAAILLYPDREQTITDLSRRLGIP
                     LATVSDEVARLVEARILTARKIGRSNLLRPNPDNRLVGPLTEIVLATMGPHLVVSDAF
                     AGMAGVSQLLIYGSWAARYHGQAGPPPNDLDLLVVGKPDRAAVYAAADVVEQRTSLPV
                     NPVIASAKRWNDDADPLVIQIKSSPSLEVHLQR"
     gene            13465..13761
                     /locus_tag="VFC01_13520"
     CDS             13465..13761
                     /locus_tag="VFC01_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80686.1"
                     /translation="MTWQSGTQEVIAALRAGELQQVTGGQAAGDGWIAQARRKYETAR
                     LISDADPETAYVTAYDAARFAVVGVLAQQGLRATQKGGHLAVEQAVRAQFGPCS"
     gene            13752..13925
                     /locus_tag="VFC01_13525"
     CDS             13752..13925
                     /locus_tag="VFC01_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80687.1"
                     /translation="MFVTFSTLRRRRAELEYPSYPGEKVDEGELLDALRTVEQIIDGA
                     AQLLPHLSIFTDA"
     gene            complement(14072..14686)
                     /locus_tag="VFC01_13530"
     CDS             complement(14072..14686)
                     /locus_tag="VFC01_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HZN80688.1"
                     /translation="MTLSAQLWADNGDLAAAALAHPFVSRLGDGSLPREIFAGYVAQD
                     AFFLESFARAYALALARSTDTPTLLALADLLGGVREELGLHSSYAARWGIDMAGVVPT
                     SATLAYTEFLLATAATGGLALVFAAMTPCMRLYAWLGASLDASAAGPFAEWVQTYADP
                     SFQTLAARLEQLLDEQDDDNPAVHATYRRAMRLELDFFESALAG"
     gene            complement(14683..15240)
                     /locus_tag="VFC01_13535"
     CDS             complement(14683..15240)
                     /locus_tag="VFC01_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family transcriptional regulator"
                     /protein_id="HZN80689.1"
                     /translation="MLDSLWSAATRHPFLEAVREGTITDSAFDRWLVQDALFVGDLLT
                     FQARLLARAPRTAQTVLAGGCVALVAELDWFEDQAARRGMNLEQPALPATLAYRELLA
                     RLDATPYEAAVTALWVLERVYLLAWAFAASATSPFGEFVAHWSAPAFADYVDALGVLA
                     IPDRHDKLVADVLAHEVAFWDMALA"
     gene            complement(15670..16456)
                     /locus_tag="VFC01_13540"
                     /pseudo
     CDS             complement(15670..16456)
                     /locus_tag="VFC01_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394755.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
BASE COUNT         2722 a         5364 c         5664 g         2706 t
ORIGIN      
        1 gaccgacagc acgggcccga agatctcgtc ggtgtagacg ctcatctcgg gcgtgacgtt
       61 gtccagcagt gtggggccga gccagaatcc ctcggcgctg ccgtcggcct gcacctggcg
      121 gccgtcgacg acgattttgg cgccgtcggc ttccccggcg tcgatgtagg aggcgacttt
      181 gtcgcggtgg gctttggtga ccagcgggcc catgtcggag tctttggtgc cgtcgccgat
      241 tttgaggccg gtggtgcgtt cggtgatttt ggcgatcagg tcgtcggcga tggggccgac
      301 ggccacacac gccgagatgg ccatgcagcg ttcaccggcc gagccgaacc cggcgttgac
      361 catcgcatcg gccgcgagat cgagatcggc gtcgggcagg atcaccgcgt ggtttttggc
      421 cccgcccagg gcttggacgc gttttccgtg cgcggtgccg gtgaaataca cgtattgcgc
      481 gatgggggtg gagcccacga aggacaccga cttgattttg gggttggtca gtagttcgtc
      541 gacggcggtc ttgtcgccct gcagcacatt gaacacacct ggcggtagcc cggcttcggc
      601 ccacaggttg gccagccaca gcgaggccga cgggtccttc tcgctcggct tcaagaccac
      661 ggtgttgccg gcggcgatcg cgatggggaa aaaccacatc ggcaccatgg ccgggaagtt
      721 gaacggggag atgatcccga ccgggcccaa aggttggcgg atcgagtaca cgtcgacctt
      781 ggtggaggcg ttctcggtga atccgccctt gagcaggtgt gggatgccgc aggcgaattc
      841 gacgacttcc tgaccgcgtg acacttcacc gagtgcgtcg gagacgacct tgccgtgctc
      901 gctggtgatg atctcggcca gctcaccctt acgggcattc aacaactcgc ggaagttgaa
      961 cagaatctgg gtgcgtttgg ccaacgacgt atcccgccac gccgggaacg ccgcagcggc
     1021 cgcgtcgatc accacacgcg catcctcgac ggtggccagc gccacctgcg cggtcacctc
     1081 accggtcgcc ggattcgtca ccggtgacgt gttggtgcca gcgcccgagt agggcttgtt
     1141 gttgatccag tggggaatgg tgttgtgcac gcctccagcg tgccttggga ggcccgctga
     1201 tgggtcgagc agagtgttaa ccgagtcgct acacgattta cactctgtaa atgcctctga
     1261 cggtggcgga agtcgtcgac ctgcccgtca tccaggctgg gcagcccgag gtgctgagcg
     1321 cccgccgatg ggacgatcag atccgctggg tgcacgtcag cgatgtcgcg gacttgtcga
     1381 ccctgttaca gggcggcgag ctggtactga caaccggcgc cgcgctgcga agatccccgg
     1441 cccgatactt gcgcggcatt gccgaggcgg gcgcgctcgg ggtcatcgcg gaactgcccc
     1501 agggcgtatc tctgccgaag acggcggcag cagcagcggc aagtgcaaat gtcgcgttga
     1561 tagtgctgca ccgcgagatc aagttcgtcg aagtgaccga ggcggtgcat cgacggatcg
     1621 tgtccgagca gttcgaagcg gtcgcgttcg accgtcgagt gcacgcgacg ttcaccgacc
     1681 tgagcatgaa acgggtctca gcgcccggga tcgtcgcggc cgccgctcgc atgctcgacg
     1741 agccggtcgt gctggaagat cttgcgcacc aagcacttgc cgtcgcgccg gggccggata
     1801 ccaccgcgac gctgctcgat gactgggaat accgctcccg acgagccacc gccgcccgtg
     1861 acggctgggc gatgactccg gtcggccccc gcggcgaaga atggggaagg ttggtcgtgc
     1921 cgcggcatcc cgtggacgcg tcgcgcgcca agatggtgct cgagcgcgcc gcggctgcac
     1981 tcgccctgaa ccggatgatc gaacgggacc ggtccgggtt gcaccagcag gcccagagcg
     2041 ggctgatcga cgacgtcatt cgcaatcggg tcaccgacga gcgcgagatc accgcccggg
     2101 cacacgcgtt aggcctccga aagacttctc ggtatcaccc ggtggtggtg cggaccgcag
     2161 acgcgtcgac gggatccgat ccggttgccg gccagcgccg caacgtcaga cttctggacg
     2221 cggtggccca caccgtgaac gccgctggcc acaccgggct gttcacgatc cgcggcgacg
     2281 gcgagatcgg cgccgtggtg gcattgagcg cgagccgggc aggcgcagca gaaaaggcgt
     2341 ggagcgcgct gggcttggac gtggcgcggg aggtagcgcg ggcggacggc ggtcagcggt
     2401 cggtactcgc attgggcgag ccgtccgggc tgatcaccga cgccatcgtc gggattgcgg
     2461 acgccgcgca cgttgccgag gcggcattgg cgatgggcgg tgagccgcga ccgttctacc
     2521 gtacctccga tgtgcggctg cgtgggctca tctcgctgct gcaggacgac ccgcgactcc
     2581 aggcgttcgc cgaaaccgaa ctcaaagcgc tgctaacagc cgagagcgcc ggtagaccaa
     2641 cggatttcac gatactgcgc gaatatctcc ggttgggcgg caacaaagtc gcgctcgcga
     2701 cccagcttcg catcagcagg ccggcgctct ataagcggct atcggccatc gagacgaaac
     2761 tcggcgtcga cctcagcgac gcagaatcga cggcgtcgct gcatgtggcg atgctggcgt
     2821 tggaagtgcg ccgcggcagc gacgtcgggg tcacaccgtc gtaggaagcg tcgcaacgca
     2881 gctcaactcg ccaacaggtc tctggcgcgt agcgccaacc agaactcggc gatggcggcg
     2941 gtatcgccga tgcgccttcc ggtgatctcc tcgatccgct gcatccgata gacaacggtt
     3001 tggcggtgaa tgccaagctc tttcgcggtc cgcaccaagg accgctcgtg tcgcaggtag
     3061 atgtcgagcg tgacaaccat gtcactggaa tgcgctacgt cgtaggcaag gaggcgaccc
     3121 agcacccggt cgaccacgac acgcgcctct tctggatcgc gcagtaccgg gaagatcgtc
     3181 gcgtcggcga accgcgcgat ctggctcgat gtcgtgacgg ccacgcgcac cgcccacgtc
     3241 gcttcgcgca ctgcgaccgg cgcacgcgac ggcagagaca gcgggtcgct gacaccgaag
     3301 atcgcctcgc cgccgagtcg ctgattcagc acgtcgagtg tgtggtcggt gacgggcatg
     3361 agcgcgtaca gcagagcggc gcggcgcagc agcagatgcg gtatcgaacg cctgccgagg
     3421 ccgaggtgca gccggcgctc gctggccgcc gaaacaccct cggccgcaac aacacaacag
     3481 ctggccgcgt cgagtccgcg ctcggctaac tcccggtcgc cgtcgcggtc gctcatgcgg
     3541 ccgtcgcaca gctggcccat cacctcggcg ccgatccgtc gttcgtgttc gcggcgcgcg
     3601 ttctgttgcg ccagcagcac cgcgaccgcc gacgccaggt gctgcaggtg gaccatgtca
     3661 gttgtcgggc cgcgaaacgc atacgtcacc agcgccgtcg gctcttcgtc gggaacttcg
     3721 acgacgaggg ctcgttggga tcgggtcgac acgtgcagca ccccggggat cgcacctcgc
     3781 cgaccgccga tctcgtcggt gatcgcctcg cgcagatcct cgggcggcgg gtcggaagcc
     3841 tccagcgcca cggcgccggt ggcggcatcc agcaccgcga ttcggcacga caggtcacgc
     3901 tccagctgcg acagcacgtt ggccggcgcc ggattggaca cggattgccg gatcacgttg
     3961 taaacccgct cggtgaccgc gacgcgtccg ctgcctgccg cgtcggcgac cgcccggccg
     4021 atcgatgcga agccgaccga atagggaacc gtcatgatgg ggaagcccag atcgtcggcc
     4081 agcgcgaccg ctgccggggt cagcgcgggg gtcgcggcgt ccaatccgat caccatgccg
     4141 cacatgccgt tctcggccac accgcggatc aatgcggttt ggccgcgggc agattccgtc
     4201 agcgtctgcc cgttcctcat cagtagctcg ccgccggcca ggtacaacca cggctcctcg
     4261 aggtccgacg tctgggccca tgtcaccggc tggtccaagc cgtcgctgcc acccttgagc
     4321 gtcagccgaa ggtggggcgt gtcgaggaga tcgcgaacgc ggtacaccat cagtcatcga
     4381 ttcgatacgt gcggttaccg cgccgacatg cggtttcagc cggtgcgaaa ggccagctca
     4441 aagccgctga acaggcgcgc ggcgccggcg gaggcggaca aaacatagtc gagcctagac
     4501 gatcgtatga actgttggct gaacttcaga ctcccgacgt attccgttaa ctgtgtgggg
     4561 cggcccacag ttgcggcggg cgagacatcc agcgaagggg agccgacatg gccgaatcca
     4621 tcgacacggc agcagctgct cctgaacggt tgcagcgcgg cttcagcttc cgctcggcgc
     4681 tgtcactggc attcgccgac gtgtcgccga tcgcggcggt ttacgcgatc ttcacgctcg
     4741 ggctgttcgc cgcggggccg aagttcttct gggcgtttcc cattgtgttg atcggccagc
     4801 tgctcgtggc cgcggtgttc ggcgagctcg cgtcgcggtg gccgtacgcc ggcagcgtct
     4861 accagtggtc gcggcacgtg cagggcacca cctggggctg ggtggccgcg tgggcttaca
     4921 tgtggggact gacgatcgcg ctcggcacgc tgtcctatgc cgcaggcggc ttcctgctca
     4981 acatcttcgg cgtcgccgaa gcaaaccgat ggcagatcgc ctcgatcgcg atcgcgatca
     5041 ttctcgtcgg aacggccgtc aacatggtcg gccgacacgt gttgaaggtc atggtcgcca
     5101 tcagcatcac ctgcgaggtc gtcggctcgc tgggcttggg cctggttctg ctgctcttct
     5161 accgcgaaaa cccgttctcg gtgctgtttt ccagcgcggg cattcccgat gcggcgtcgt
     5221 gggcgtcggg cccgatgctg ctcgccatcg cctatgtcgg ctggtcgttt ctcgggttcg
     5281 aggcggccgg atcagtcgcc gaggaggtcg acgagccgga gcgcaacgtg cccaaggcga
     5341 tcatcctggc gctgctgctt gtcggtctgg tggtgatgtt ctccgctgcg gcccttatcc
     5401 tttcgatccc aaacctgccc ggtgtcgtcg ccgcgcagtc cggcgacgtg gtcgccgata
     5461 ccctcgccac gcatctcggc cagggggtga ccaaaccgct gctggccatg ttcgtcatcg
     5521 gattcgtatc gagcttcctg gccgtccagg ctgcggtgtc acggtgtatc tggggcgcgg
     5581 cgcgcgatca cagcctgccc ggatccaagg tgctgggagg actggcgggg ccagaacgct
     5641 tgccggtcaa cgccatcgga ctcaccgccg tcatcgcgat cgtgttcgtg ctgctcgcag
     5701 gcacacagtt ctacaacatc ctggtgaact tcaacatcat cggcttctac atcgcgttcg
     5761 gcgtgccagt catcggcgcc gccctcgcgc ggttgaccgg caaatggaaa cccggcccgt
     5821 tcaccctcgg tcggtggggg gcaccgatca cctacctcgc atcgctgtgg atcatcttcg
     5881 agacagtgaa cgtggcatgg ccgcgcaccc agccggacca accctggtac atcaactggg
     5941 ccagcgtgct gacgaccgtg gtgctggggg tcatcggagt ggcgatctac ctgtcggtgc
     6001 gtcgtggcat tcaggcgccc atcgccgaac gcctcggcga tgccccggcg ccgcagtgag
     6061 ctccgtcgca atcgtcaccg gcgcggcgtc cggtatcggg gcctccgtcg tggccgctct
     6121 cgacgaagag ggttatcacg ttgtggggct tgacgttaac gactcgtcca cctacgtcgt
     6181 cgacgtctcc gatgcaggcg ccgttgatga ggtgatgcgc cgcgttgctg ccgaacacgg
     6241 tccggtcgac gcggtggtca gcgccgcggg gcactacgag tcggttccga tcgccgacat
     6301 cacccgcgac cagttgcacc gcatgctgcg cgtacacctg ggcggtctgc gcaacgtggt
     6361 gcgggcggtg ctgccgtcga tgatcgagcg gcgcgcggga gccgtcgtcg cgatcacttc
     6421 ggaactggct gtgggcggtg gcgacgcgga tgcgcattac gcggccgcca agggcgccat
     6481 catcgggctg gtgcgcagcc tggcggccga ggtcgcaggc tacgggatcc gggtgaatgc
     6541 ggttgcaccc gggcccactg acacgccgct gttggccgcc gactctcctt ggcgcgcacc
     6601 ggattatctc gcgacgctgc ccgctggtcg gctcgcgcgg ccggtggaaa tcgccgaggt
     6661 agtgcggtat ctcgtcggcg ccgacgctgc gttctgcacc ggcgaagtgc tgtccccgaa
     6721 cagcggggcg gtgatctgag atgtcacggc attctggtcg cctcgccctg gttaccggcg
     6781 cgacgcaggg gatcggcgcg gctctcgccg agcggttggc cgcagagggc gcgacggtcg
     6841 gcgtcaacgg tcgggtgcgc gacgcgcgta tggattccgt cgtcacctcg acgggcggat
     6901 tccccgcggt ggccgacgta tcggaccccg acgccgtcgg cgcgctggtc gccgacatcg
     6961 agcgcacgcg agggccggtg gacatcctgg tgtgcaacgc cgcgtatatg accatggcgc
     7021 ccttcgtcga ccacgccgat tacgactggt ggaagatcat cgacaccaac ctggcgggca
     7081 cggtgtatct ggtgcaggcg gtgctcgccg gcatgcgcag gcgaggcgcc ggcaacatcg
     7141 tcatcatcgc ctccgaatgg ggcgtcatcg gatggcccaa taccaccgcg tactcggcgt
     7201 ccaaggccgg tctgatcgcc ctgacgaagt cgctgggccg cgagctggcg cccgacggca
     7261 tcaccgtcaa cgcgatcgca ccaggcgtca tcgacactcc gcaactgcag gtcgacgccg
     7321 acgatgcagg catcagtctc gagcagatgc acgagcagta cggacgcgaa actccgttgg
     7381 ggcgcatcgg taaaccgaca gagatcgcgg ccatggtgtc actgctggcg cgcgcggaca
     7441 tgggcgcctt cgtaggccag acgatacaga tcaatggagg gacgacacgg tgccggacat
     7501 gaccgaacac catcagcccg ttgagcccgt cgacggcact gtggtgcccc gctatgcggg
     7561 cttctcgacg ttcgcgcggc tgccccgcat cgacgacgtg cccacatacg acgtcgctgt
     7621 cgtgggaatt ccgttcgacg cgggagtcag ctaccggccg ggcgcccggt tcgggcccgg
     7681 ccacgtgcga cagtgctccc gagtcctgcg gccatacaat ccacagatgc aggtgtcgcc
     7741 gttcgccaac caacaggtgg tcgacgccgg cgatatccct tgtaccccat acgacatcgc
     7801 caccgcggcc cgccagatcg aggaacatgc gaccgcgctg acggccaacg gcgcgcggct
     7861 gatcaccatc ggcggggacc atacgatcgc ctacccgctg ctgcgagtgc atcgcgagcg
     7921 ctacggaccg gttgcgctgg ttcacttcga tgcgcacctg gacacgtggg acacctactt
     7981 cgacgctccc ttgacgcacg gcactccgtt tcgccgcgcc gccgaggacg gcctcttcgt
     8041 gtccggtcac agcgcacacg tcggaatccg gggatcgcac tattcacccg acgatctgac
     8101 gtcggacgca gaactcggat tcacggttgt gcactgcagg gaattccaga agcgctccgt
     8161 cgacgacgtc gtcgggcagc tgcgcgagac catcggcgac cttccgctct acgtctcgat
     8221 cgacatcgac gtcctggacc cggcgcatgc ccccgggacg ggcacacccg agatcggggg
     8281 cctgacgagt cgcgagctgc tcgagctggt acggggattc gacggcctga acctcgtcgg
     8341 tgcggacatc gtcgaagtgg cacccgccta tgaccacgcc gagctcaccg gtatcgcggc
     8401 ggcgtgtctg gcatacgaat tcctcgccct gctggcccgg gcgcggtgtc gagcatgacc
     8461 gtccctccgg tcccgtggcc caacggtgcg cggtgcgcag cctcgttctc attcgatgtc
     8521 gacgcagagt cggccatgct gggtgtggat acctcgcacg ccgaccgaat gtcggcgatc
     8581 agccatcagg cctacggccc gctctctggc gtaccaagat tgctgaaaat ccttgccaag
     8641 cgcggcatta ggtcaacatt cttcgtgccc ggctacaccg cggtgcgcta cccggacgtg
     8701 atccgctcca tcgtcgagca cggccatgag gtcgctcacc acggctacct gcacgaggcg
     8761 atgcgtggcc tcaccgaagc gcaggaggcc gccatcctcg accgtggcct agaagccctg
     8821 gagtcggtga cggggacgcg ccccacaggg tatcgagccc cgatgtggga gttgaactgg
     8881 cattctccaa agttgctgcg agacagaggc tttctgtacg acagcagcct gatggacacg
     8941 gaccacccgt atgagctgac cgtcgacggc gacagctcgc tggtggagat tccgatccag
     9001 tgggcgctcg atgactggga gcagtactgc tacatcccgg agttcagcgg ctcgggactg
     9061 atcgagagcc cgaccaaggc gatcgagatg tggcggctgg agttcgacgc aatccgtgcc
     9121 gagggtggct gcttcgtgct gaccaatcac ccgttcctgt ccgggcggcc atcacgggcg
     9181 caggcactgg atgggctgat tgagtatgtg tgcgcgcagg gcgatgtgtg ggtggcaagc
     9241 ctgggggaga tcgctgatca tgtgcgcggc ctcggtctga ctccgcgttc cgtcgagcgc
     9301 ccgaaccccg ccgatttcta gtccgctgga ggatcgctcg gctgacggcg cagaattcgg
     9361 agcgtagcgg tcggcgcgga gaactcctcc gtcggtggtg ctgggatcac cgacgtcgaa
     9421 tcctccttga gccgaacaat tttcggtatc gctgaggtcg gtgcaggctg cccgagcgtc
     9481 ggtgccgcgg cggtcggcgg ctcaggtgag ggcggcgcgg gcggcgcggt tgggacgcgg
     9541 gtttcgcggc ggcgcaccaa gtagcccgcc aacgggccgg tgaacagcat ggcgatcagc
     9601 acggtgaggc tcagcgcgca gatgaccaca tcgaacgtgc tggtgatctg ctgggcgagg
     9661 ttgttgccgt agacggcgtc gggtgacggt tcggcgtacc gcggggcgag ggcgacgccg
     9721 atggccaggt tcgcgacggt gaagacgaac agcactccgc tgaaggccgc gatgacggtc
     9781 ggcgtggtct cgcctcggcc gagctggcgc tgcagccacc gcgcgatgat gccggtgatc
     9841 acgttgaaca ccgtcatcgc cacgctgtcg tacgcagcgc gtggcagatc cagcgacagt
     9901 gcgatcagat agtcgacgac gcggcatgcg gccgcggcaa atgcccagaa cgcgatgagt
     9961 gtcaggcact tctgcgctgc cgagcggtac gcaccgaccg tcggcggttt gatcaggaag
    10021 acggcgtaga gcgtcgtcgg ggcgacgatc gccgccgcgg ccgcccagaa cagataaccc
    10081 tcgtaaccga cggcggcggt cgacgtgttc tgggcgatgc cgtgaaaggc gtcgacgtcg
    10141 cggccgaccg ggaagaccca cacgagcgtg gtggccacta ccgccgacgc gcccagtgcg
    10201 gtggtggcca gtcgggcggc ggcggacttc tcaagaagcc agcgcgatgc aatcaccaac
    10261 gcgatcaacg cgaccgcgcc gtagagcaat gtggtgatga tgacggcgac gtcgtgaccg
    10321 ccgaaatcgc cgttcgtgac gaacagatag cgcagccgcc aatacaggtt gaaggcgacc
    10381 gagagcgttg ctagcgcaat cgagaccgca cccagcgccc gcgcggcggt ataccagcgc
    10441 cgaaaaccgt tgtcctcgat ggtgatactg gtgatcggcg gctgcgctgc gagcagcgca
    10501 cctccgacgc ccacgatcag accggggccg acgcccggcg gcagcaaccc ggttccgccg
    10561 tcccgcacgg tctgcagcaa gtggtacccg acgaagccga gggcgaggac gaggtaggcg
    10621 acgttgagga gaagccgcat caggctggtc cggcggacgt cgggctgcgg ggcggcgagc
    10681 cggaacgggc cgacatgtgg ggccagcgcg gcggccacgg cgagcaacgt cacgaagata
    10741 accacgacca agaacaggcc gttgctgccg gggatgccga cgccgacctc gaggtcccac
    10801 ggcagcagca gcgcgaggag gagcagcacg accgcgacgc cgtcgcggac gatgttggtg
    10861 cgggcgggca cgtagcccac gcggggcgac cgggcgacgg caggcgctgc ggtggtggcc
    10921 accgcgacgg tggcgggacc gtcgtaacgc tggtcgctca agtgacgatc ctttccgaag
    10981 agacaccgtg aggtgtggct acctcagcgt gctcattttt gcttcggttc acaagcaatg
    11041 ccgaggtagc tttggaacct cgtgcgaggt aaggagtggg gtcggtggac gtagtcctcg
    11101 gggtggcggt caccgggcgc gtggcgcgcc tggcgatgat cggctcgccg gcctcgggcg
    11161 gccaggtgct cgaccagtat gcgttggaca tgcctgacga cgcggcaacc gatctagccg
    11221 acacgatcgt gggtacgtac cgtgcggtgg ccgactcggg caatcgcatg gccgcgattc
    11281 ggctctgcat gccagacgcg tcgcaggccg agaccctgcg gcagacagtg ttgggcgcgg
    11341 gagcgcagaa cgtcgatgtg gtggcggaga ccgaggccgc cgcggcgctg gcgcgcagtg
    11401 tcggtgcgga cgcggcactg ttgctggccg acgacgacac cgtgtcgctg acggtggtcg
    11461 gcgaggacga ggagtcgacg tcggtgctgg cgtcggcgcc gatcggtgtg gccggtgcgg
    11521 ccgtcgcgtg tgcggcggtg ctgcagaagg tgccgagcgc ggacgcgccc gtgcggatca
    11581 tgctggtcgg tcaacgcttg gatctggatt cggttgcggc agagctgcgt tcgacggcgc
    11641 cggtggaggt gccgcccgat gcgggctacg cgatcgcgcg gggcgcggcg cagacggccg
    11701 aggagtccgg ctacccggca ggcgcggcga cgcaaatggc gcccgcggcc gcggacgcga
    11761 cgatgatggc gcctgcttcg gacgcgacga tggtggcgcc cgcggctggt gacgcgacgc
    11821 agatggcgcc gtcgacggat cccgcggcgg tcgggccgca gctggcgtac tcacaagagg
    11881 agccgggcga ttacgagctg ccgttcgatt cgctcgagga attcgttccg gagctggagg
    11941 acgaagaggc gtatacggcg atgatcgcgc cgccaccgcg taagacgctg ttgatgggca
    12001 gcgcgctggc gttcgtggtg gtgagcttcg cgacgcttgc ggtgacggtc gcgatcaaca
    12061 tccggccggt ggcagatgtg gaggcgcagg cggtgccggc gactcaggcg gaaactgtgc
    12121 ctgggcgatt tctgccgcag gtgccgcacg agccggatcc ggtggcgctg ccggttgcgg
    12181 tggtgtcgcc ggcgcccgcg gctccggcac cgagtgtgcg gacgaaccgc gggccggtgg
    12241 acaacaatgt gccgtcggcc ccggctcctc aggctccggc gcctgaaccc gctccggcgc
    12301 cgggagtgcc ggtggtcccg attccgccgg tcatcccgtt gccgccgctc ccgacgttgc
    12361 cgacgttcac gacgacagtc acgacaacgc cgacgacgac aacgaccacg ccgacgacca
    12421 caacaacgac gcccacgacc acgacgacca cgccgacgac gacgtctacg gagacgacga
    12481 cgtcgacgtc agagcccacc acgattccgc tgtcgccggt cacgccggtg attccgaaga
    12541 cagagttgcc ggcgacgcag gcacccgcgt acacgcctgc tccggcgtac acgccaccgc
    12601 cggagcctgc ctacacgccg cccgctgtcg cgcctgttgc gccagcgcct gctccggttg
    12661 cgccagcacc tgctcccgtc atcgagaagc ctgcgccagc cccggtgatt gaggcgcccg
    12721 tgacgactat cccggtgtca ccctaatcag acgctggccg gtacccgatc cgccttcgcg
    12781 cgcatctcgc gggcgcatag ttgccctgaa acatccgaac acgttgcgaa cccgtagcac
    12841 tgctacgcat aaatcggaga tatcatcgga ttatgcgtag cagtgcaccg gctttgtttc
    12901 cggtgttcag gtccccgtcg caggccgaga tcctcgctgc gattctcctg tacccggacc
    12961 gcgagcagac catcaccgac ttgtcgcgcc ggctcggtat tccgctggct accgtctccg
    13021 acgaggtcgc ccgcctggtc gaggcgcgca tcctgaccgc gcgcaagatc ggccgctcca
    13081 atctgctgcg cccgaaccct gacaaccgac tcgtaggtcc cctgaccgag atagtcctgg
    13141 caacgatggg cccgcatcta gtagtgagcg acgctttcgc cggcatggcg ggtgtcagcc
    13201 agttgctcat ctacggctcg tgggccgccc ggtaccacgg ccaggcgggg ccgcccccta
    13261 acgacctcga cctgctcgtt gtcggcaagc ctgaccgcgc cgcggtatac gccgccgccg
    13321 acgtcgtcga acagcgcacc agcctgccgg tgaaccctgt catcgcgtcg gcgaagcggt
    13381 ggaacgacga cgccgatccg ctcgtcatac agatcaagtc cagccccagc ctcgaggtcc
    13441 atctgcagcg ataggagttg actcgtgact tggcagtccg gcacgcaaga ggtcatcgca
    13501 gccctgcgtg ccggcgaact gcagcaagtc accggaggcc aagctgccgg tgatggctgg
    13561 atcgctcaag cgcgacgcaa gtacgagacg gcacggttga tctctgacgc cgatccggaa
    13621 acggcgtacg tcaccgccta tgacgcggcc cgtttcgccg tggtcggagt gcttgcgcag
    13681 caaggcctcc gggcgaccca gaagggtggg catctcgctg tcgaacaggc agtccgcgct
    13741 caattcggtc cgtgttcgtg acattctcga cgctgagacg gcggcgggcc gaactcgagt
    13801 acccctctta cccaggtgag aaggtcgacg aaggagaatt gctcgatgca ttgcgcacag
    13861 tcgagcagat catcgatggg gcagcgcaac tgctgccgca cttgagcatc ttcaccgacg
    13921 cctgatctcg cgcgacaaca acggcttcag acgccggccg ttccccgttc cgccgtcgcg
    13981 cgcatctctc gacccgcgat gacctgattg ccggccgttc ggtatcgatc ggctaagtcc
    14041 gcgggtcggc cgcagctcgt cgagacgacg ctcagcctgc gagcgccgat tcgaagaaat
    14101 ccagctccag gcgcatcgcc cgccggtagg tcgcgtgcac ggcgggattg tcgtcgtcct
    14161 gttcgtcgag cagttgctcc agccgggcgg ccagtgtctg gaagctcggg tcggcgtagg
    14221 tctgcaccca ctccgcgaac ggtccagcag cgctcgcatc aagcgatgca ccgagccagg
    14281 cgtacaggcg catgcacggc gtcatcgccg cgaacaccaa ggccagcccg ccggtggcgg
    14341 cggtcgccag caggaattcg gtgtaggcca gggtcgccga tgtcggcacg acaccggcca
    14401 tgtctatgcc ccaccgggcg gcgtaggacg agtgcaggcc gagttcctcg cgcaccccac
    14461 cgagcagatc ggccagcgcg agcagcgtgg gtgtgtcggt actacgagcg agggccaatg
    14521 cgtatgcgcg ggcaaaagat tcgaggaaga aggcgtcctg cgcgacgtag cccgcgaaga
    14581 tctcacgggg cagggagccg tcaccgaggc ggctcacgaa agggtgcgcg agcgccgcgg
    14641 ccgccaagtc gccgttgtcg gcccacaact gagcggacag cgtcatgcca gcgccatgtc
    14701 ccagaacgcc acctcgtgtg ccagtacgtc ggcgactagc ttgtcgtgtc ggtccgggat
    14761 ggccaggacg ccgagcgcgt cgacgtaatc cgcgaaggct ggcgcggacc agtgcgctac
    14821 gaactcaccg aacggggaag tggccgacgc ggcgaaggcc catgcgagca ggtacacccg
    14881 ttcgagcacc cacagtgctg tcaccgccgc ctcatacggc gtggcgtcta gtcgcgccag
    14941 cagttcgcgg taggcgagcg tcgccggtag cgccggctgc tccaggttca tcccccgccg
    15001 cgcggcctgg tcctcgaacc agtccagctc cgcgaccagc gccacgcatc ccccggcgag
    15061 caccgtctga gctgttcgcg gcgctcgtgc cagcagccga gcctggaagg tcagcaggtc
    15121 accgacgaac agcgcgtcct gcaccagcca ccggtcaaac gcggagtcgg tgattgtgcc
    15181 ctcgcgcacc gcttcgagaa acggatgccg ggtagcggcg gaccacagcg aatcaagcac
    15241 gccccgaagg atagtcacgc tgacgactgg ggcgacagcg cgtcggtctc gacacctggc
    15301 tgccggacca agctaccttc tggctttaga aggcttgatc agccagagag ccaaaacggt
    15361 cggcacggat gtgacgggtc agctcagcgg tgagtgacga ccggcggccg cgggcagggc
    15421 acaggccgat ctgcccgagt agcgagcgac aagcgcgtct cagcaggtga gcgccataga
    15481 gcgtgcggct cgaacggcga caacgcacga cttgcgccgc aggtcaacaa gttgtcgcgc
    15541 ataggtgggc accaaggcga tcgcgcatag agcgacgcgc caggtctcgc gtgtgcttgc
    15601 tgaactcatc gatctcgagg tcgcgtcggt gtggcgccgt catgtcgcgg ccaggttgat
    15661 gaccgtattt cagactcccg ctggcacccg gtccgcggtc gcgcgcatct cccgccggag
    15721 cgacgtgaac tcggagatcg tcctggtcgc cacagacgcg caggtcgggc gttgcggccg
    15781 gtgggttcgg tcttggacac catcaccacg ctgtcgatgt agggctggat ggtggtggcg
    15841 ttctgcaccg tcgcgacgat caccagctgg aagccgaact tgcggaatgc ctgcaaagcc
    15901 tgttgggcga actgcgggtc ggacttggag aatgcctcgt cgagcatcag ctgcgcgaaa
    15961 accggtgtgt tgtcggcact ttcggggctg gccaggttga agctcagtgc accggcgagg
    16021 cagaacgcca tcagcttctc ctgctcgccg ccggagttgt cgccggcatt gctgtgcgtg
    16081 cggatcagtt catcgttggc gacgtcccac tcggcgcaat cgaaggtgaa ccggttgcgc
    16141 acgtccagcg cgtcgcgcgt ccacgccttg tcctcgggcg cggtggacgc cagccggttg
    16201 cgcagccgca ggatgtcggc gtactggtcg aggatcgcct gcttgtcgcc taggccgacc
    16261 tcggcgatgc gccgcgagat ggaccgcacg acctcggtga gctcggcgac ggccggcagc
    16321 tgccgcggag tggcgcgcag cgtgaggcgg gtgccgcggt tgaactccac tgcgcccaaa
    16381 ccggtgttga ctcggtcgat ctggtcgctg atgcgccggg cctcctgctc ggcgacgcgg
    16441 tgcagcgtga ggatcg
//
LOCUS       DASRJA010000312         3189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3785139,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000312 DASRJA010000000
VERSION     DASRJA010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3785139"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VFC01_13545"
     CDS             complement(<1..1214)
                     /locus_tag="VFC01_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HZN80690.1"
                     /translation="MGFTKPDFPDVDPDAFMQKPLMERMRILATDWVDHGFGSPRMVH
                     TIYIAKLLFFYALGGVLVATLTSGLPLLRVSQWWNQPIVYEKAVLWTVLLELIGVAGS
                     WGPLAGKIKPMTGGILFWARPGTIRLRPWKWVPLTSGDRRTWFDVGLYIVLMISVALP
                     LFSPGVHSDSLSAAMPGNTSGLVNPTLMIAPIVLLVIMGLRDKIVFLAARGEQYLPAL
                     IMFAVFPFVNMIIALKLLIGVVWVGAGVSKLGLHFTNVIPPMVSNSPFIPFKWLKRAH
                     YRNYPDDLRPSHLASFMAHVPGSVVEILAPLALLFSTNKWVTIVAAVIMVCFHLFIIS
                     TFPLAVPLEWNVLFAYATVLLFLGFPAWNGYALWDMSPAWLALVVAAALLFYPILGNF
                     RPDKVSFLPSMR"
     gene            complement(1251..2714)
                     /locus_tag="VFC01_13550"
     CDS             complement(1251..2714)
                     /locus_tag="VFC01_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN80691.1"
                     /translation="MNLSALPDQRAAENPLAPAVADDKTDLDNAQFLAAVQRTAASLR
                     GHGVSAGDVVGIMLPNRASFVVSLFAAWRLGAAVTPINPSLRPAEVTYQVSDAGAKVL
                     IVEQTPGFDAGVAAVEIDQLDCGDATPGGDALHEADDALALLIYTSGTTGRPKGVMLD
                     HANLDAMCNGVIDAFKLTGADHSLLILPLFHVNGIVVGTLSPLLAGGRATVAGRFRPE
                     SFFDRIEQSRPTYFSAVPTIYTMLCGLPASVKPDTSSVRFAICGAAPASVELLEAFES
                     RYGIPIIEGYGLSEGSCASTVNPLAGERKPGTVGLPVPGQTIRIVDTAGRPVPDGQPG
                     EVVIKGPNVMRGYLNRPEETTKTIVDGWLHTGDVGRLDDDGYLVLVDRAKDMIIRGGE
                     NIYPREIEAVAHQLPQIAEAAVVGRANAVYGEEPVLFVSLHPQQVLDTDEIRDHLSDS
                     LSKYKLPVDITILEDLPKNAVGKIDKPELRKRLVETR"
     gene            2888..>3189
                     /locus_tag="VFC01_13555"
     CDS             2888..>3189
                     /locus_tag="VFC01_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HZN80692.1"
                     /translation="MRRDDDVDVSRYVAETADRLHDRLAAVSSGIQSLLEDDIPELRG
                     DARVMELLGASVEGNVETLLHALRYDIAVERVVPPTAALEYARRLAQHGVPVNALVR"
BASE COUNT          591 a          999 c         1055 g          544 t
ORIGIN      
        1 tgccgcatcg acggcaggaa cgacaccttg tccggtcgga agttgcccag gatcgggtag
       61 aacagcaatg cggctgcgac cacgagcgcc aaccaggccg gggacatatc ccagagcgcg
      121 tagccattcc aggccgggaa gccgaggaac agcaagacgg tcgcgtaggc gaacagcaca
      181 ttccactcca gcggcacggc cagcgggaac gtcgagatga tgaacaggtg gaagcacacc
      241 atgatcaccg cggccacgat ggttacccat ttgttcgtgg agaacaacag ggccagcggg
      301 gccagaattt cgaccacgct gcccggaacg tgcgccatga agctggcgag gtgcgaaggc
      361 cgcagatcgt cggggtagtt gcgatagtgc gcccgcttca accacttgaa cggaatgaac
      421 gggctgttgc tcaccatcgg cgggatgacg ttggtgaagt gcaggcccag cttcgacacc
      481 ccggcgccga cccagacgac gccgatcaac agtttcagcg cgatgatcat gttgacgaac
      541 gggaacaccg cgaacatgat cagggcgggc aggtattgct caccccgggc cgcgaggaac
      601 acgatcttgt cccgcagtcc catgatcacc agcaggacga tcggcgcgat catcagcgtc
      661 gggttgacca gccccgaggt gttgccgggc atcgccgcag agagtgaatc gctgtgcacc
      721 ccaggtgaaa acagcgggag ggcgacactg atcatcagca cgatgtagag gccgacgtca
      781 aaccaggtcc gtcggtcgcc gctagtcaac ggaacccact tccacggccg cagccggatg
      841 gtgccaggcc tggcccagaa caggatgcca cctgtcatcg gcttgatctt gccggccagc
      901 gggccccacg atccggcgac gccgatcagt tccagcagca cggtccacag cacggccttc
      961 tcgtagacga tgggctggtt ccaccactgc gatacacgaa ggagcggaag cccggacgtc
     1021 aacgtcgcga ccaggacgcc gccgagcgcg tagaagaaga ggagcttcgc gatgtagatc
     1081 gtgtgcacca tccgcggcga cccgaaccca tggtcgaccc agtcggtggc gaggatccgc
     1141 atccgctcca tgagtggctt ctgcatgaag gcatccggat cgacgtcggg gaagtcaggc
     1201 ttggtgaatc ccatgatcag ctctcctttg tgcgtgatgg gctttgtgtt tcaacgtgtt
     1261 tcgacgagcc gcttgcgtag ctcgggtttg tcgatcttgc cgaccgcatt cttcggcagg
     1321 tcttcgagaa tcgtgatatc aaccgggagt ttgtatttcg acagcgagtc gctgaggtga
     1381 tcgcgaatct cgtcggtgtc gagtacctgt tgagggtgta gcgacacgaa caacacgggc
     1441 tcttcgccgt agaccgcgtt ggccctgccg acgaccgcgg cctcggcgat ctgggggagt
     1501 tggtgcgcca cagcttcgat ctcgcgcggg tagatgttct cgccgccgcg gatgatcatg
     1561 tccttggctc ggtcgaccag aaccaggtaa ccgtcgtcgt ccagtcggcc aacgtctccg
     1621 gtgtgcagcc agccgtcgac gattgtcttg gtggtctctt cgggccggtt caggtagccg
     1681 cgcatcacgt tgggtccctt gatcacaacc tcaccgggct ggccgtcggg caccggacgc
     1741 cccgccgtgt cgacgatccg gatggtctga cccggcacgg ggagtcccac cgtgccgggc
     1801 ttgcgctcgc cggccaacgg gttgaccgtg ctggcgcagg atccctctga caacccgtac
     1861 ccctcgatga tcgggatgcc ataccgggat tcgaacgcct ccagcagttc gacgctggcc
     1921 ggtgccgccc cacagatggc gaagcgcacg gacgaggtgt ccggtttcac gctggcgggc
     1981 aagccgcaca gcatcgtgta aatggttggt acggcggaga agtaggtggg gcggctctgc
     2041 tcgatccggt cgaagaacga ctcgggcctg aaccggcccg cgacggtcgc tctgcccccg
     2101 gccaacagcg gtgagagggt cccgacaacg attccgttga cgtggaacag cggcagtatc
     2161 agcaggctgt gatcggcacc ggtcagcttg aacgcgtcga tcaccccgtt gcacatcgcg
     2221 tcgagattgg cgtgatcgag catcacgccc ttcggccgac ccgtcgttcc gctggtgtag
     2281 atcagcaggg ccagcgcgtc gtcggcctcg tgcagggcat cgccgcccgg cgttgcgtct
     2341 ccgcagtcga gctggtcaat ctcgactgcc gccacccccg cgtcgaaccc gggggtctgc
     2401 tcgacgatca gcaccttggc accggcgtcg gagacctggt aggtgacctc cgcgggccgg
     2461 agcgacgggt tgatcggtgt caccgccgcg cccagccgcc atgctgcgaa caacgacacg
     2521 acaaagctgg ccctgttggg cagcatgatg ccgacgacgt cgccggccga taccccatgg
     2581 ccacgcaacg acgccgcggt gcgctggacc gcggcgagga actgggcgtt gtcgagatcg
     2641 gtcttgtcat cggcaacggc gggcgcgagt gggttttccg ccgcgcgctg atcgggaagt
     2701 gcggataggt tcatgtgcgc tcctcgttcg cagttgagtg gcgataacgg taggaatccg
     2761 gcgatccgcg acacagtcgc gaacccataa gaaccgactc gggttttgtc gcctggagac
     2821 aacatgtgag ccctgcaaat ctccggctat cgggggcgcg acgatctact gtcatgaggt
     2881 gaccaccctg cgtcgcgacg atgacgtcga cgtgagccgc tacgtcgccg agaccgccga
     2941 ccggctgcac gaccggctgg cggcggtgtc gtctggcatt cagagtttgc tggaggacga
     3001 catccccgag ctgcggggcg acgcgcgggt catggagttg ctgggcgcca gcgtcgaggg
     3061 aaacgtcgaa acccttctgc acgcactgcg ctacgacatc gccgtggagc gggtggtgcc
     3121 gccaacggcg gcactggagt atgcgcgccg gctcgcccaa cacggcgtcc cggtgaacgc
     3181 gctggtccg
//
LOCUS       DASRJA010000313         3725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4132795,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000313 DASRJA010000000
VERSION     DASRJA010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4132795"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFC01_13560"
     CDS             <1..326
                     /locus_tag="VFC01_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HZN80693.1"
                     /translation="EPTRTPSGYRNYGDDAVRVVRFVKRAQQLGFTLDDIEDLLHLAG
                     GGPDSCDEAKAMARSRIDDLQLRIDELVGMRDALTRLIETCGESRAERECPILHEIET
                     AVGVK"
     gene            329..586
                     /locus_tag="VFC01_13565"
     CDS             329..586
                     /locus_tag="VFC01_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylmercury lyase"
                     /protein_id="HZN80694.1"
                     /translation="MRIELLTSPGCPNAAAAKRTVTDCLATLGIDAPIIERVGGYASP
                     TVLVDGVDVMRPEDGVFRGDVCRLDLPTPQRILHALQKEPT"
     gene            583..1242
                     /gene="merB"
                     /locus_tag="VFC01_13570"
     CDS             583..1242
                     /gene="merB"
                     /locus_tag="VFC01_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005179592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase MerB"
                     /protein_id="HZN80695.1"
                     /translation="MTADLFGPDHEAGRLLMRTAVRLLARGEPVTKSELAAAAGCDVT
                     DLIDAPAGPDIEYDDEDRIVGWGLTLNSTPHSFVVDGHRLYTWCAADTLMFPAIIGRR
                     AEIESRCPTTGTVIRLTVDPRDGVSDVSPASAVISIPNPEELDVSRVRASCCNPGRFF
                     ASAEAAEDWLAQYPGGTVLPVADAYPQLHPIGRRLLDRALVPGFRAAKDPPYGGGRRP
                     D"
     gene            1217..1753
                     /locus_tag="VFC01_13575"
     CDS             1217..1753
                     /locus_tag="VFC01_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZN80696.1"
                     /translation="MAAAAGPIDRAKIAADLERARLEFHRLLAEADRADAWAKPTRET
                     RWTNEQLLFHMVFGYMVVLRLLILVRVFSRLPDPVNRAYARALDSGTTPFHVINYYGS
                     CAAALVYNRRRMGAKLDRVIASLQRKLARESDGAFDRGMHFPPRWDPFFTDYMTLADV
                     YRYPGQHFDFHARQLALG"
     gene            complement(1750..2184)
                     /locus_tag="VFC01_13580"
     CDS             complement(1750..2184)
                     /locus_tag="VFC01_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN80697.1"
                     /translation="MANKALRKFKFERRVGRTVVNPLVAALERIGIRSSLVVELETIG
                     AKTGQPRRVPLTGRADDKGVWVISQHGRRAGWAHNIAANPQVRVRMDDQWRSGTATFE
                     PDDDVRARARSFGGNGKLSQSAAALTMRAMESDPISVRITYD"
     gene            complement(2343..3362)
                     /locus_tag="VFC01_13585"
     CDS             complement(2343..3362)
                     /locus_tag="VFC01_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN80698.1"
                     /translation="MPTDLWEIAAWALACSVPVVLAGAIIIRLARSWSLAVSMVALVL
                     IPVLATFTGVLGASGFMITETFERTAIVLVIVSVVTLPAAVMLARYQARRTVWEQEIR
                     DSERAAEQSRRRLVAFVSHDLRTPLAGIRAVSEAIADGVVPDAEVRVHAKTIEQESIR
                     LSEMVDDLFEMSKINAGAVEPAFDKVALDEVVDDVLTAHRITAERAGVLLTAELPEKP
                     VRVLGSDRALVRVLSNLVANAIAHTPEGGAVSLSLGSDENGAWARVDDTGVGIDAADL
                     PRVFDVAYRGSNGRVPRADSSLPSGSGLGLAIAAGLVQAHRGTLSAHNLTTGARFEVR
                     LPVAD"
     gene            complement(3352..>3725)
                     /locus_tag="VFC01_13590"
     CDS             complement(3352..>3725)
                     /locus_tag="VFC01_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN80699.1"
                     /translation="FSPRELALRVRSVLRRAPSQSGVLPLDITVGDMTVSTAARSVMV
                     DGEPVSLTNREFDLLLYFLTHTDTVFSREELLKRVWRWDFGDLSTVTVHVKRLRTKLG
                     DHHRVQTVWGRGYLWSGSSAD"
BASE COUNT          666 a         1302 c         1134 g          623 t
ORIGIN      
        1 ccgagccaac gcgcacaccg tcggggtatc gaaactacgg cgacgacgcg gtgcgagtgg
       61 tgcggttcgt caagcgcgcc caacaactcg gcttcactct cgacgacatc gaggatctgc
      121 tgcacctagc cgggggaggt cccgactcgt gcgacgaagc caaggcgatg gcccgtagtc
      181 ggatcgacga tctgcaactg cgcatcgacg aactcgtcgg catgcgtgac gcgctgaccc
      241 gtctgatcga aacctgtggc gagtcccgcg ccgaacgtga gtgcccgatc ctgcacgaga
      301 tcgaaactgc ggttggcgtg aaatgaccgt gcgtatcgaa ctcctcacgt cgccgggttg
      361 cccgaatgcc gcagccgcca agcggaccgt caccgactgt ctggccaccc tgggcatcga
      421 cgcgccgatc atcgaacggg tcggtggtta cgcctcgccg acagttctcg tcgacggtgt
      481 ggacgtcatg cgtccggaag atggggtttt ccgcggcgac gtgtgccggc tcgatctgcc
      541 cacgccgcaa cggattctcc acgcactgca gaaggaaccg acatgaccgc cgacctattc
      601 gggcccgacc atgaggccgg gaggctgctg atgcgcaccg cggtgcggct acttgcccgt
      661 ggcgaaccgg tgacgaagag tgaactcgcc gccgccgcag gctgcgatgt caccgatctg
      721 atcgatgcac cagcaggccc agacatcgaa tacgacgacg aggatcgcat cgtcgggtgg
      781 ggcctcacac tcaactcgac gccgcacagc ttcgtcgtcg acgggcatcg cctctacacg
      841 tggtgcgctg cggacactct gatgttccct gcgatcatcg gtcgccgagc agagatcgag
      901 tcacgttgcc ccacaacggg taccgtgatc cgtttaactg ttgacccgcg agacggagtc
      961 agcgacgtct ccccggccag tgcggtgatc tcgattccca accccgagga gctggatgtc
     1021 agccgggtgc gtgccagctg ttgtaacccg ggtcgcttct tcgcaagcgc ggaggcggcc
     1081 gaggactggc tggcgcagta ccccggcggc acggtgctgc cggtcgccga cgcatatccg
     1141 caactgcacc cgattggtag acggctgctc gatcgagcgc ttgtgcctgg cttcagggcg
     1201 gcgaaggatc ctccatatgg cggcggccgc cggcccgatt gaccgcgcaa agatcgcggc
     1261 ggatctggag cgcgcccggc tcgaattcca tcggctcctc gccgaagcgg accgtgccga
     1321 tgcgtgggcc aagccaaccc gcgagacacg ctggaccaac gagcagttgc tgttccacat
     1381 ggtgttcggc tacatggtcg tgctgcggct gctcattctc gtgcgggtgt tcagccgcct
     1441 acccgatccg gttaatcgtg cgtacgcccg cgcactcgac agcgggacta cgccgttcca
     1501 cgtgatcaac tattacggat cctgcgctgc cgccctcgtc tacaaccgcc gccgcatggg
     1561 cgccaaactc gaccgggtca tcgcgtcatt gcaacggaag ctagcccggg aaagtgacgg
     1621 agccttcgac cgcggcatgc atttcccgcc gcgatgggac ccgttcttca cggactacat
     1681 gacgttggcc gacgtctatc gctatccggg tcagcacttc gattttcacg cacgccaact
     1741 cgcactgggc tagtcgtagg tgatccgcac cgagatcggg tcgctttcca tggcgcgcat
     1801 cgtcaatgcg gcggcggatt ggctgagttt gccgttgccg ccaaagctgc gagcgcgagc
     1861 acgaacatcg tcgtcgggct cgaaagtggc tgtgccgctg cgccattgat cgtccatgcg
     1921 gacgcgcacc tgcggattgg cggcgatgtt gtgtgcccag ccggcgcgac ggccgtgctg
     1981 ggagatcacc cagacaccct tgtcgtcggc ccgaccggtg agaggcacac gccgcggttg
     2041 accggtcttc gcgccgatgg tctccagttc gaccaccagt gacgagcgga tgccaatccg
     2101 ttccaacgcc gccaccagcg gattcacgac ggtgcgaccc acccgtcgct cgaacttgaa
     2161 cttgcgcagc gccttgttgg ccatcgccta ccccatcgtc attaggttta cgacgttaat
     2221 agaacgttcg ctcaaaaaca aggcggcgtg aaccacacat acaaaattgc ccgcgtaggt
     2281 agcgacaatc cggctgtcgc tcgctacgcg ggcactttcg cacacgtacc cgggagccgc
     2341 gcctagtccg ccaccggcag ccgcacctcg aatcgcgctc cggtcgtcag gttgtgtgcc
     2401 gacaacgtgc cccgatgtgc ctgcaccaat cccgccgcga tcgccaaacc cagaccggaa
     2461 ccgctcggca acgaggagtc cgctcgcggc acccggccgt tcgacccgcg gtacgccaca
     2521 tcgaacaccc gcggcaggtc ggccgcatcg atacccacac cggtgtcgtc gacgcgcgcc
     2581 cacgcgccgt tctcgtcgga ccccagcgac agcgacactg cgccgccctc gggggtatgt
     2641 gcgatcgcgt tggcgaccaa gttggatagc acccgcacca gcgcccggtc gctgccgagc
     2701 acccgcaccg gcttctccgg cagctcagcc gtcaacaaca ctcccgcgcg ctccgccgtg
     2761 attcggtgcg cggtcagtac atcgtcgacg acctcgtcca gcgccacctt gtcgaacgcc
     2821 ggttccaccg cgcctgcatt gatcttcgac atctcgaaca gatcgtcgac catttccgag
     2881 agccgaatcg actcctgttc aatggttttc gcgtgtacgc ggacctcggc atcgggcact
     2941 acgccgtctg caatcgcctc cgacaccgcg cggattccgg ctagtggtgt gcgcaggtcg
     3001 tggctgacga acgcgaccaa ccggcgccgc gactgctcgg ccgcccgctc ggaatcgcgg
     3061 atctcctgct cccacaccgt tcgtcgggcc tggtagcgcg ccagcatcac cgcggcgggc
     3121 agcgtcacca ccgagacgat caccagcacg atcgccgttc gctcgaacgt ctcggtgatc
     3181 atgaacccgc tggcgcccag cacgccggtg aacgtcgcca acaccggaat gagcaccagc
     3241 gccaccatgc tgaccgccag cgaccacgac cgcgccagcc ggatgatgat cgccccggca
     3301 agcacgacgg gcaccgagca ggccagcgcc cacgccgcta tctcccacaa gtcagtcggc
     3361 actgctaccg ctccacaggt agccgcgccc ccacaccgtc tgtacccggt ggtggtcgcc
     3421 gagtttcgtc cgcagccgct tgacgtgcac ggtgaccgta gacaagtcac caaagtccca
     3481 gcgccacacc cgtttcagca gttcttcgcg agagaacacg gtgtcggtgt gggtgaggaa
     3541 gtacagcagc aggtcgaatt cgcggttggt caggctgacc ggctcgccgt cgaccatcac
     3601 cgagcgggcg gccgtcgata ccgtcatgtc gccaacggtg atatcgagcg gcagcacccc
     3661 tgattgcgac ggcgcccgcc gcagcaccga gcgaacccgc agcgcaagct cacgcgggct
     3721 gaacg
//
LOCUS       DASRJA010000314        11440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1727949,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000314 DASRJA010000000
VERSION     DASRJA010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11440
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727949"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VFC01_13595"
     CDS             complement(<1..1114)
                     /locus_tag="VFC01_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZN80700.1"
                     /translation="MTYIVNGNTFDEDPRPGQCLRTFVRSLGYHGVKKGCDAGDCGAC
                     TVWLDGVPVHSCITPAFRAEGREVTTIEGLGTPDNLHPLQRQFGDAPGFQCGFCTAGV
                     IMTAATFTEDQKNDLPRALKGNLCRCTGYRAIEDAINGVTAIEQAQPGQAVGTSVGAP
                     AATDVVTGRAEFTMDTEIEGMLHLKVLHSPHAHARIVSIDKSAALAVPGVHRVYTWED
                     VPRKRYTTAIHTDHLVDPDDTYILDNVVRFAGQRMVAVVADTVAAAEEGCSKVVIEYE
                     PLPAVFEPEEAMAPGAPQLHGSDDPFVRDPERNILLELHGEIGDIEAGFAQADVIHEG
                     TYFSPRVQHAHLETHGSIAWMDDDRLIVRTSSQSPSI"
     gene            complement(1111..1935)
                     /locus_tag="VFC01_13600"
     CDS             complement(1111..1935)
                     /locus_tag="VFC01_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZN80701.1"
                     /translation="MDVNTVSEVVRQPQERPGTAWREGDAWLAGGSWLFSEKQPAVRR
                     LIDLVPLGWDSLDATDAGLEIGTMCTIAQLYAFRGPDWRAAPLFATCCEAFLASFKVW
                     HSATVGGNICMSLPAGPMITMTVALEAAYTLWAPNGSERIVDSVDFVTGNHANVLAPG
                     ELLRKIDIPVRALNKRHAHRRFTLTKLGRSTIFMIGTQSPDTADLMLTITAATAHPIR
                     LAFDSIPDADALQARIDQLPDEVWFDDPNGAPDHRRHLAKHYAEEIRMELATGASR"
     gene            complement(2437..3048)
                     /locus_tag="VFC01_13605"
     CDS             complement(2437..3048)
                     /locus_tag="VFC01_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80702.1"
                     /translation="MLDSAGQPDVGAGKRGRPRSEQSHAAVLRATSELLHEVGLRSMT
                     TEEIAARSGVSKATIYKWWPNKYAVAVEAFLSEMMAESHDPHTGSAATDLRIVLRGLV
                     NFYTESNGRVFAELIGEAQFDPAVRTELHKHLMISHRDLVKTVWARGVATGQFRADID
                     PDDAIDVLIGPLLYRRLLGQPHLDGTAADTLVDVTMRGLSVRL"
     gene            complement(3230..3859)
                     /locus_tag="VFC01_13610"
     CDS             complement(3230..3859)
                     /locus_tag="VFC01_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN80703.1"
                     /translation="MNGGPIQRVVTHGVFELDGGSWEVDNNIWVVGDDSEVVVFDAAH
                     DAGPIVMAVGGRHVVAVVCTHGHNDHVTVAPELGEALDAPVLLHPADDVLWRMTHSDK
                     DFRAVNDGDTLRVAGHELRAIHTPGHSPGSVCWHAPELNAVFSGDTLFEGGPGATGRS
                     YSDFPTILESISGRLGQLPGDTVVYTGHGDSTTIGDEIVHYDEWVARGH"
     gene            complement(3856..4944)
                     /locus_tag="VFC01_13615"
     CDS             complement(3856..4944)
                     /locus_tag="VFC01_13615"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HZN80704.1"
                     /translation="MTQTVRGVISRKKGEPVEVTDVVIPDPGPGDVVVDILACGVCHT
                     DLHYREGGINDEFPFLLGHEAAGTVESVGDGVTHVEPGDFVILNWRAVCGECRACKRG
                     RPWYCFNTFNASQPMTLTDGTELTPALGIGAFADKTLVHELQCTKVDPQADPAVAGLL
                     GCGVMAGLGAAVNTGAVGRDDTVAVIGCGGVGDAAIAGARLVGAKRIIAVDTDNKKLD
                     WARGFGATHTINARELDPVETIQDLTDGNGADVVIDAVGRPETWKQAFYARDLAGTVV
                     LVGVPTPDMTLEMPLIDFFSRGGSLKSSWYGDCLPERDFPTLISLYLQGRLPLEKFVS
                     ERIGLDDIEHAFHKMHAGEVLRSVVVLK"
     gene            4982..5752
                     /locus_tag="VFC01_13620"
     CDS             4982..5752
                     /locus_tag="VFC01_13620"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HZN80705.1"
                     /translation="MIPALYLSHGAPPLVDDALWVEQLKKWASELPTPTAILVVSAHW
                     EHSPLTIGSTTPETPLTYDFWGFPEHYYQVTYDAPGAPELAARIEALMPDGEPVAHDS
                     DRRLDHGAYVPLTVMYPDATIPVLQISMPTLDPQRLLALGTRLRPLRDEGVLIIGSGF
                     TTHGLPFLDDPSPDASPPDWSTEFDAWANERFAAGDVDALIDFRHQAPGMPYAHPTIE
                     HFSPLFVALGASSDPAQSPQQVIDGYWMGLAKRSIELT"
     gene            5771..6844
                     /locus_tag="VFC01_13625"
     CDS             5771..6844
                     /locus_tag="VFC01_13625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN80706.1"
                     /translation="MTTMNENPVDTTEEFAERIVGAIDDASLAILLSIGHQTKLFDTM
                     TEMPPATSAQIAEAAGLNERYVREWLGGVVANRVVDYDPAAQTYALPGHRAAVLTRAA
                     GPDNLSRVAQFIPLLGEVEQKIIGCFHNGGGLPYSDYPRFHKLMAEESGEVFDAALVD
                     IILPLVDGLPDRLRAGADLADIGCGSGHAVNVMAKAFPASRFTGIDFSDEGLAVGAAE
                     AKSLGLTNATFVAADVTNLDSAETYDVITVFDAIHDQAQPARVLQNIYRALRPGGVFL
                     MVDIKASSNLEDNIGVPLSSFKYTVSTMHCMSVSLGLDGVGLGTVWGQQLATSMLADA
                     GFGEVTVSEIESDPFNNYYVARK"
     gene            complement(6849..7280)
                     /locus_tag="VFC01_13630"
     CDS             complement(6849..7280)
                     /locus_tag="VFC01_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN80707.1"
                     /translation="MTSIQNSTVTDISTAERLSEALAEVFRTAEMGDVLTDDVFLDGN
                     PPLWRFQLQGIEAFAEWYKSYAPNGMDEVTVVRTVPTATGFVAELTERVRNDGKEITG
                     REIVLCTVRDGRISELTVYCTGDWDEELRARHAAEAPMLRP"
     gene            7481..7876
                     /locus_tag="VFC01_13635"
     CDS             7481..7876
                     /locus_tag="VFC01_13635"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN80708.1"
                     /translation="MSEHPNVTTVNRMTEAIVEEDRETLASLFTDDFVFHLRGPFATA
                     GDHLGVAGILGAIRPVFEATGGDVKLEQQFCVGVDGWAAEWEHAVLGRHGKTLESDNA
                     FVYRFVGGRIAEMWMFLGADPDTAVAFFG"
     gene            7914..9521
                     /locus_tag="VFC01_13640"
     CDS             7914..9521
                     /locus_tag="VFC01_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZN80709.1"
                     /translation="MGGPSQSRGMAWGEEYRQLAGLDAEQGLDVDDLDRLATAAYMTG
                     RDDESFEFWGRGHHRCLEIGDMARAARFGVRLAQALGFKGDIARSSGWAERSHRLLDD
                     ADLDCVERGFLEYAAGMVRIFADGDLAAAHSAFGRAAKIGERFRDRELLTFARMGVGR
                     CLIYLGEIAEGLALLDEAMVSVEAREIPPMAVGDAYCTAIDACYELFDIRRCEQWTNS
                     FTRWCDAQSGLVLYRGHCLLHRAELLMLHGDWSDAVTVAQEACARLAEPINPLTLGGA
                     HYVEAELHRLRGDFSMAEGAYERANAEGCEPQPGMALLRLAQGRVDVAAAQLRRLLAE
                     ADQTIARARVLCAATEILLAAGDVEGARAAADELSAVATELGSPLLRAHAALATGSVL
                     LGAGDAPTALVALRRAARDWSELDAPYERARTRLLIADACEALSDLDGAELERRAAGA
                     TFDALGAGAAPSRRTTSPSAGVDLTAREAEVLALVAQGKTNRAIAAELCISEKTVASH
                     LNHIFTKLGLASRSAATAYAYQHDLMA"
     gene            9532..10356
                     /locus_tag="VFC01_13645"
     CDS             9532..10356
                     /locus_tag="VFC01_13645"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN80710.1"
                     /translation="MSALDGMADWPVESAAAAVVTPSGVVARYGDTKQRFALASVTKP
                     LVARAGQIAIEEGVAELDTEAGPPGSTVRHLLAHAAGYSMNSAELMAKPGQRRVYSNY
                     GFRVLAETIQQSSGIDFAQYLAEAVFEPLGMTDSALEGGADAAGFGATSTVADLSAFA
                     SDLLRPNTVSPQMHAEAIEVQFPGLTGVLPGFGTQRPNDWGLGFEIRDAKSPHWTGAS
                     NSARTYGHFGQSGTFLWVDPGADMALVVLTDRNFGEWAYPLWPALSDEVLREFAGN"
     gene            10464..10976
                     /locus_tag="VFC01_13650"
     CDS             10464..10976
                     /locus_tag="VFC01_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HZN80711.1"
                     /translation="MRAANQFADAATGVVYIHASPAAVCPHVEWALSSTLQARANLRW
                     TPQPAMPGQLRAITNWIGPVGTGAQLANALRSWSVLRFEVTEDPSEGVDGHRWCHTPQ
                     LGLWSGAMSANGDVMVGEMRLRGLMASGADVLAAELDSVLGTAWDEALEPYRDGGDAA
                     EVSWLSRGVG"
     gene            complement(10987..>11440)
                     /locus_tag="VFC01_13655"
     CDS             complement(10987..>11440)
                     /locus_tag="VFC01_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560758.1"
                     /note="involved in the biosynthesis of
                     phosphatidylinositol mannosides (PIMs); the enzyme from
                     Mycobacterium tuberculosis can phosphorylate a variety of
                     amphipathic lipids; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HZN80712.1"
                     /translation="TDRTNRMTWPHGRMRYNVAMVAELSKLRLLPFRLTFDGRELETE
                     LTLAAFGNTRSYGGGMLICPDADPTDGQLDVTMVQSASRTRLIRLFPTVFKGTHVDLD
                     EVRTDRARTITVDSPGINAYADGEYVCPLPVEVSAVPAALKILTPHPG"
BASE COUNT         1917 a         3709 c         3797 g         2017 t
ORIGIN      
        1 cgatcgacgg cgactgcgag cttgtgcgga cgatcagccg gtcgtcgtcc atccaggcga
       61 tcgacccgtg cgtctccaga tgggcgtgct ggacccgggg cgagaagtac gtgccttcgt
      121 ggatcacgtc ggcttgtgcg aagccggctt cgatgtcgcc gatctcaccg tgaagttcca
      181 gcaggatgtt gcgctcggga tcgcggacga acgggtcatc ggatccatgc agttgcggag
      241 ccccaggtgc catcgcctct tcgggctcga aaaccgctgg cagcggctcg tattcgatga
      301 ccaccttgct gcagccctct tcggcagcgg ccactgtgtc ggcgaccacg gcaaccatcc
      361 gttgaccggc gaaccgcacg acgttatcga ggatgtacgt gtcgtcggga tcgacgaggt
      421 ggtcggtgtg gattgccgtg gtgtagcgct tacgcgggac gtcctcccag gtgtagacgc
      481 gatgcacgcc cggcacggcc agcgcggcgg acttgtcgat ggacacgatg cgggcatgcg
      541 cgtgcggcga gtgcagcacc ttcaggtgca gcatgccttc gatctcggta tccatggtga
      601 attcggcccg ccccgtgacc acgtcggtgg ccgccggtgc gccgacgctg gtgccgactg
      661 cctgcccggg ctgtgcttgc tcgatggcgg ttacgccgtt gatcgcgtct tcgatggcgc
      721 gataacccgt gcagcggcag aggtttcctt tcagcgcgcg ggggaggtcg ttcttctgat
      781 cttcggtgaa ggtcgccgcc gtcatgatca cgcccgcggt gcagaagccg cactgaaatc
      841 cgggggcatc gccgaactgg cgctgcagcg ggtgaaggtt atccggtgtg ccgaggcctt
      901 cgatcgtcgt gacttcacgg ccctcggcgc ggaaggccgg ggtgatgcaa ctatgcacgg
      961 gtacgccgtc gagccacaca gtgcaggcgc cgcaatcgcc tgcgtcgcaa ccctttttga
     1021 cgccgtgata cccgagtgag cgtacgaacg tccgcaggca ctggccgggt cgcgggtcct
     1081 cgtcgaatgt gttgccattg acgatgtagg tcatcgcgag gctcccgtcg cgagttccat
     1141 tcgaatttcc tctgcgtagt gcttggccag gtgacggcgg tggtcgggtg cgccgttcgg
     1201 gtcgtcgaac cacacctcat cgggcagttg gtcgatacgc gcctgcaatg cgtcggcgtc
     1261 gggtatggag tcgaacgcca agcggatcgg atgagccgtc gccgcagtaa tcgtcagcat
     1321 gaggtcggcc gtatcgggcg actgggtacc gatcatgaaa atcgtcgacc ggccgagttt
     1381 ggtcagtgtg aagcgccggt gtgcgtgacg cttgttcaag gcgcggaccg ggatgtcgat
     1441 cttgcgcaga agctcgccgg gagcgaggac gttcgcatgg ttgccggtga cgaagtcgac
     1501 gctgtcgacg atgcgttcag aaccgttggg agcccacagc gtatacgcgg cttcgagcgc
     1561 caccgtcatc gtgatcatcg gtcctgccgg caacgacatg cagatgttgc ctccgaccgt
     1621 cgccgagtgc cagactttga atgacgccaa aaatgcctca cagcacgtgg cgaacagcgg
     1681 ggccgcgcgc caatcggggc cgcggaatgc gtacaactgc gcaatggtgc acatcgtgcc
     1741 gatctccaac cccgcatcgg tggcgtcaag cgagtcccac ccgagcggta cgagatcaat
     1801 caatctcctt acggcaggct gtttctcgga gaacagccag ctgccgccgg ccagccacgc
     1861 atcaccttcc cgccacgcag tccccggtcg ttcctgcggt tgtcggacca cttcactgac
     1921 ggtgttgacg tccattcagc tactcctcat ggatgtgctg gggccgcagc gctggggcta
     1981 ttgcgcacgg tcgcccctga ctgagacgcc agtactgttt gctgcggcca ccgtcaaacc
     2041 cttgctttac gtcgcgaaga agttatggag tttcgggcgt cggacatctc ccggacgtcc
     2101 gactgcggcg aaggctagca cagacgtcaa atagctggca atatctaaac gccgcgtttc
     2161 tcaaatctgg ttggtgtttc aaaagacatg gtcatccacc aagttttcgc aagtttgcct
     2221 accgttcacg aggatctcgc ggtctctaca gcaagctgac agggaaggcg taagcctcca
     2281 gcaggcagga aacttctcag gatctgtgcg ccacggcacg cctgatgtag gcctaacgta
     2341 tgaatgcgca ataattgctg agtcaataaa ctcgaatttg ccccgaaagc ggtcgcttgg
     2401 gttatagtgc atcgcccaag cggccgtcgg cgagtttcac aatctgacgg acagcccgcg
     2461 catggtgacg tcgacgagag tgtcggctgc ggtgccgtcg aggtgtggtt gaccgagaag
     2521 gcgacggtag agcaggggtc cgatcaggac gtcgatcgcg tcgtcggggt cgatatcggc
     2581 gcggaattgg ccggtggcga ccccgcgggc ccacactgtt ttgaccaggt cccgatgcga
     2641 gatcatcaag tgcttgtgca gttcggtgcg caccgccggg tcgaattgcg cctcgccaat
     2701 cagttcagcg aacacgcggc cgttggattc ggtgtaaaag ttgaccaggc cgcgcaacac
     2761 gatgcgcaaa tccgttgcgg ccgaaccagt gtggggatca tgcgactcgg ccatcatctc
     2821 cgacaggaag gcctccacgg cgacggcgta cttgttcggc caccacttgt agatcgtggc
     2881 cttgctgacg ccactgcgcg cggcgatctc ctcggtcgtc atcgaccgca gtccgacttc
     2941 gtgcagcagt tcggaagtcg cccgcagcac cgccgcgtgc gactgctcgc tgcgtggtcg
     3001 ccctcgcttg ccggcgccca catcaggctg ccctgcgcta tccagcatcg cactcccgtc
     3061 cccgtgaccc aggaacgatc acggtgcacc taccggattc ttgcacgggc ctatccgcca
     3121 ggagcgcatg agaatgcccg aggaaaccag gaatgaggtc agttcgacgc tgggcgagcg
     3181 cgctgatctg cctgccggcg ttgcggccgc catgcccgca cggtagccat taatggcctc
     3241 gggcaaccca ttcgtcgtag tgcacgattt cgtcaccgat ggtggtggag tcaccgtggc
     3301 cggtgtagac gacggtgtca cccggtaact ggcccagccg gcccgagatc gactcgagga
     3361 tggtagggaa gtcggagtag gaccggcccg tcgcaccggg cccgccctcg aacagggtgt
     3421 cgccgctgaa cacggcgttg agctcggggg catgccagca caccgagccc ggtgaatgcc
     3481 ctggcgtgtg tatcgcgcgc agttcgtggc ctgcgacgcg cagggtgtcg ccatcgttga
     3541 ccgcacggaa atccttgtcc gagtgtgtca ttcgccacag cacgtcgtcg gcagggtgga
     3601 gcagcacagg tgcgtccagg gcctcgccaa gttcgggcgc gaccgtcacg tggtcgttgt
     3661 ggccgtgcgt gcacaccacc gcaaccacat gacgaccgcc gacggccatt acgatcggtc
     3721 cggcgtcgtg ggccgcgtcg aacaccacga cctccgagtc gtcgccgacg acccagatgt
     3781 tgttgtcgac ttcccaactg ccaccgtcga gttcgaacac cccgtgggtg acgacccgct
     3841 gaatcggacc gccgttcatt tcagcaccac caccgagcgc agcacctcgc cggcgtgcat
     3901 cttgtggaag gcgtgttcga tgtcgtcgag cccgatgcgc tcggagacga acttctccag
     3961 cggcagcctg ccctgcagat acaacgagat cagggtgggg aagtcacgct cgggcagaca
     4021 gtcgccgtac cacgacgact tcagcgatcc gccgcgcgag aagaagtcga tcagcggcat
     4081 ctccagcgtc atgtcgggcg tcggaacgcc aaccagcaca acggttcccg ccaggtcgcg
     4141 ggcgtagaac gcctgcttcc aggtctccgg ccgcccgacc gcgtcgatca ccacgtcggc
     4201 gccgttgccg tcggtcagat cctgaatcgt ctccaccgga tctagctcac gcgcgttgat
     4261 ggtgtgcgtg gcaccgaaac cccgcgccca gtcgagcttc ttgttgtcgg tgtccacggc
     4321 gatgatccgc tttgccccca ccaggcgcgc acccgcgatc gcggcatccc cgacaccacc
     4381 gcagccgatc accgcgactg tgtcatcgcg accgaccgcc ccggtgttga cggctgcacc
     4441 aaggccggcc atcacaccgc aacccaacag cccggccacc gccggatcgg cctgcgggtc
     4501 gaccttggtg cactgcagct cgtgcaccag cgtcttgtcg gcgaacgcgc cgatgcccag
     4561 cgccggcgtc aattccgtgc catcggtcaa tgtcatcggc tgcgacgcgt tgaacgtgtt
     4621 gaagcagtac cacggccggc cgcgcttaca cgcccggcat tccccgcaca ccgcgcgcca
     4681 gttcaggatc acgaaatcac cgggctcgac gtgcgtgacg ccatcaccga ccgactcgac
     4741 ggtgcccgcg gcctcgtgcc cgagcaggaa agggaactcg tcgttgatgc caccctcgcg
     4801 gtagtgcagg tccgtatggc agacgccgca ggcaaggatg tcgaccacga catcgcccgg
     4861 cccggggtcc gggatgacga catcggtcac ctcgaccggc tcgccctttt tccgtgagat
     4921 cacaccgcgc accgtctgag tcatgcgtac aacttagtgc gaacggtgga aggctagggg
     4981 cgtgatcccg gccctctact tgtcgcacgg cgcacctccg ctcgtcgacg acgcgctctg
     5041 ggtcgagcag ctgaagaagt gggcaagcga gttgccgaca ccaaccgcga ttctcgtcgt
     5101 ctccgcgcac tgggagcact cgccgctgac catcggctcg accactcccg agaccccgct
     5161 gacgtatgac ttctggggat ttccggagca ctactaccag gtcacctacg acgcacccgg
     5221 cgcgcccgag ctcgccgctc gcatcgaggc gctgatgccc gacggcgaac ccgtcgcgca
     5281 cgactcggac cgccggctcg accacggggc ctacgtcccg ctcactgtca tgtatcccga
     5341 cgcgaccatc ccggtgctgc agatttcgat gcccacgttg gacccgcagc gcctcctcgc
     5401 gctcggcacc aggctgcgtc ccctgcgcga tgaaggcgtc ctgatcatcg ggtcgggttt
     5461 caccacgcac ggcctgccgt tccttgacga cccgtcgccc gacgccagcc cgccggattg
     5521 gtcgaccgag ttcgacgcgt gggccaacga gcggttcgcc gccggcgacg tcgacgcgct
     5581 catcgacttc cgccaccagg ccccgggcat gccgtacgcc cacccgacca tcgagcactt
     5641 ctcgccgttg ttcgttgcgc tcggtgcttc cagcgaccca gcgcaatcgc cgcaacaggt
     5701 catcgacggg tattggatgg gcctggcgaa acggtcgatt gaactgacct gagtcgccta
     5761 gcgttttccc atgaccacca tgaacgagaa cccggtggac accaccgaag agtttgccga
     5821 acgaattgtc ggggcgatcg atgacgccag cctggccatc ctgttgtcca tcgggcatca
     5881 gaccaagttg ttcgacacga tgaccgaaat gccgccagcc accagtgcac agattgccga
     5941 ggccgcggga ctcaacgaac gatatgtgcg ggagtggctc ggcggcgtgg tcgcgaatcg
     6001 ggtcgtcgac tatgacccgg cggcgcagac gtatgcgctt ccggggcatc gcgcggcggt
     6061 gctgacccgc gccgccgggc cggacaacct gtcgcgcgtt gcgcagttca tcccgctgct
     6121 cggtgaggtc gagcagaaga tcatcggctg cttccacaac ggcggtggac tgccctacag
     6181 cgactatccg cggttccaca agctgatggc cgaggagagt ggcgaggtgt tcgacgccgc
     6241 gctggtcgac atcatcctgc cgctcgtcga tggattgccg gatcggcttc gcgccggtgc
     6301 ggacctcgcc gacatcggtt gcggcagcgg acacgccgtc aacgtgatgg cgaaggcgtt
     6361 ccccgcgagc cggttcaccg gcatcgactt ctccgacgag ggcctggcgg tgggtgccgc
     6421 ggaggcgaag agcctcggcc tgacgaacgc gacgttcgtc gcggccgatg tgaccaacct
     6481 cgactcggcc gaaacctacg atgtgataac ggttttcgac gcgatccacg accaggctca
     6541 gccggcgcga gtgctgcaga acatctaccg cgcgctgcgg ccgggtggtg tcttcctcat
     6601 ggtggacatc aaggcatcga gcaatctgga agacaacatc ggtgttccgc tgtcttcttt
     6661 caagtacacc gtctcgacga tgcactgcat gagcgtgtcg ctggggctcg acggcgtcgg
     6721 gctcggcacg gtgtggggcc agcagctcgc gacatcgatg ctcgccgacg ccggattcgg
     6781 cgaagtgacg gtcagcgaga tcgagtcgga cccgttcaac aactactacg tcgctaggaa
     6841 atgaccggtc acgggcgcag catcggcgct tcggctgcgt ggcgggcgcg cagttcctcg
     6901 tcccagtcgc cggtgcagta gacggtcagc tcggagatcc ggccgtcgcg gacggtacag
     6961 aggacgatct cgcggccggt aatctccttg ccgtcgttgc gaactcgctc cgtgagctcg
     7021 gcgacgaatc ccgtcgcggt gggcacggtg cgcactacgg tgacctcgtc catgccattg
     7081 ggtgcgtagc tcttgtacca ctcggcgaag gcctcgatgc cctgcagctg gaaccgccac
     7141 agcggcgggt tcccgtcaag aaatacgtcg tcggtgagga cgtctcccat ctcggcagta
     7201 cggaatacct cggcgagtgc ctcgctgaga cgctcggccg tgctgatgtc ggtcacggtt
     7261 gagttttgga tgctcgtcat gtcggttccc tttctcgtca gtccgaccgt aggcacaggt
     7321 gcccgagcgg cacatcgagc gaactcccga tttgatgcgc gctgccggat ccacagaatt
     7381 accgatgcac gccttgcgat ttccatggtt tgcccgatgt gcgagtaagg cagggctcgt
     7441 agcgtacgga ccgaaaccgc ttgagcggag ggaggaaccg atgagcgagc acccgaacgt
     7501 gacaacagtg aatcggatga ccgaggccat cgtggaggag gaccgcgaga cgctcgcgtc
     7561 gttgttcacc gacgacttcg tcttccacct gcgtggcccg ttcgccaccg ccggcgacca
     7621 cctcggagtg gcgggcatcc tcggcgcgat ccgcccagtc ttcgaggcga ccggcgggga
     7681 cgtgaagctc gagcagcagt tctgcgtcgg cgtcgacggc tgggcggctg agtgggagca
     7741 tgccgtgctc ggtcgtcacg gcaagacact cgagtcggac aatgcgttcg tctaccgatt
     7801 cgtcggcggc cgcatcgccg agatgtggat gttcctgggg gccgaccccg acactgccgt
     7861 cgcgttcttc ggttaacgcg cccgccgact gatgtttcca ccacactgga ggggtgggtg
     7921 ggccgagcca aagccgcggc atggcatggg gcgaggagta ccggcaactt gccgggctcg
     7981 atgccgagca aggactcgac gtcgacgatc tcgaccgatt ggcgaccgcc gcgtacatga
     8041 ccgggcgcga cgacgagagc ttcgagtttt ggggccgggg acatcaccgc tgcctcgaga
     8101 taggcgacat ggcccgggcg gcccgctttg gcgtgcggct cgcccaagcg ctcgggttca
     8161 aaggcgacat cgcccgcagc agcgggtggg ccgagcggtc acaccggttg ctggacgacg
     8221 ccgatctcga ctgcgtcgag cgcgggttcc ttgagtacgc ggccggcatg gtccgcatct
     8281 tcgccgacgg cgacctcgcg gcggcgcact ctgcgttcgg gcgggccgcc aagatcggcg
     8341 agcgttttcg cgaccgggag ctgctcacgt tcgcgcggat gggggtgggc cggtgcctga
     8401 tctacctcgg cgagatcgcc gaaggcctgg cgctgctcga cgaggccatg gtgtcggtcg
     8461 aggcgcgcga aatcccgccg atggcagtcg gagacgcgta ctgcaccgcg atcgacgcgt
     8521 gctacgagct gttcgacatt cgccgctgtg aacagtggac caactcgttc acacggtggt
     8581 gcgacgctca gagcgggctt gtcctgtatc ggggccactg cctgctgcat cgcgccgagc
     8641 tgctgatgct gcacggtgac tggtccgacg ccgtgaccgt cgcgcaggag gcgtgtgccc
     8701 ggctcgccga accgatcaat ccattgaccc tcgggggcgc tcactacgtc gaggccgaac
     8761 tgcatcggtt gcgcggtgat ttctcgatgg ccgagggtgc ctatgaacgt gcgaacgccg
     8821 aggggtgcga accccaaccc ggcatggcgt tgctacggct tgcgcagggg cgcgttgacg
     8881 tcgccgcggc ccagctccga cgcctgctcg ccgaggccga ccaaaccatc gcccgggctc
     8941 gtgttctgtg tgcggcgacc gagatcctcc tcgccgcagg cgatgtcgag ggggcccgcg
     9001 ctgctgccga tgagctgtcc gcagtcgcca cggagctggg ttcaccattg ctgcgggccc
     9061 acgccgcact agcgactggc tccgtccttc tcggggccgg cgacgcgccc actgcgttgg
     9121 tggccctgcg ccgcgctgcc cgagattgga gcgaactgga cgcgccgtac gaacgggcgc
     9181 gtacccgcct gctgatcgcc gacgcctgcg aggcactttc ggacctggac ggtgctgagc
     9241 tggagcggcg cgccgccggt gcgacgttcg acgctctcgg cgcgggcgcc gccccgtcga
     9301 ggcgcacgac aagcccgtcg gctggggtcg atctcaccgc ccgcgaggcg gaggtcttgg
     9361 ctctcgtcgc gcagggcaag accaaccgcg ccatcgcggc ggagctgtgc atcagtgaga
     9421 agaccgtcgc cagccatctg aaccacatct tcaccaagct cggactcgcg tcccggtcgg
     9481 ccgcgacggc atacgcgtat caacacgact tgatggcata ggctcgcgtc gatgtccgcc
     9541 ctcgacggca tggccgactg gccggtcgaa tccgccgccg ctgccgtcgt caccccgtcc
     9601 ggtgtggtcg cgcgatacgg cgacacgaag caacgcttcg cgttggcgtc ggtgaccaaa
     9661 ccgctcgtcg cccgcgcggg gcagatcgcg atcgaagagg gtgtggccga gctcgacacc
     9721 gaggccggcc cgccggggtc cacggtccgg cacctgctcg cccatgccgc ggggtactcg
     9781 atgaactcgg cggagctgat ggccaagccg ggccagcgcc gggtctactc caactacgga
     9841 ttccgggtgc tcgcggagac gatccagcaa tcgtcgggca tcgatttcgc gcagtacctg
     9901 gccgaggcgg tgttcgagcc gctcggcatg acggattccg cgctggaggg cggcgcggac
     9961 gcggcgggat tcggggcgac gtcgacggtc gccgacctgt cggccttcgc tagtgacctg
    10021 ctgcggccca acacggtgtc cccgcagatg cacgccgagg ccattgaggt gcagtttccg
    10081 ggtctgaccg gggtattgcc gggcttcgga acgcagcggc ccaacgactg gggtctggga
    10141 ttcgagatcc gcgacgccaa gtcgccgcac tggaccggag cgtccaactc ggcccgcaca
    10201 tatgggcatt tcggccagtc agggacgttt ttgtgggtcg atccgggcgc ggatatggcg
    10261 ctcgtggtgt tgaccgaccg caacttcggg gagtgggcct atccgctctg gccagccctg
    10321 tctgacgaag tcctgagaga atttgccggg aactagcgca acacgggtct cacaaggcac
    10381 aatagacacg cacgacacag ctgtaattcg gattcaccag gtcgttgggg aagacgtccc
    10441 tcgtggaacc gaaggagcag ctgatgcgtg cagcgaacca attcgccgac gcggcgacgg
    10501 gtgtggtgta catccatgcc tcacccgcgg cggtatgccc gcatgtcgag tgggcgttgt
    10561 cgtcgaccct tcaggcaagg gcgaacctcc ggtggacccc gcagcccgcc atgcctggac
    10621 agctgcgcgc gatcaccaac tggatcgggc cggtcggcac cggggcgcag ctggccaacg
    10681 cgctgcgctc gtggtcggtg ttgcggttcg aggtgaccga ggatcccagc gaaggcgtgg
    10741 acgggcaccg ctggtgccac accccgcaac tgggactgtg gagcggcgcg atgagcgcga
    10801 acggcgatgt gatggtcggc gagatgcggc tgcgcgggtt gatggcgtcg ggcgccgacg
    10861 ttctcgccgc cgaactcgat tccgtgctgg gcaccgcctg ggacgaggcg ctggagccct
    10921 atcgtgacgg tggcgatgcg gctgaggtca gttggctgag ccgcggagtc ggttagccga
    10981 cgacattcag ccggggtgtg gggtaaggat cttcagcgcg gcgggcaccg ctgacacctc
    11041 gacaggcagc gggcacacgt actcgccgtc ggcataggcg ttgatgccgg gggagtccac
    11101 ggtgatagtg cgggcgcggt cggtgcgcac ctcgtccagg tcgacgtggg tgcccttgaa
    11161 taccgtgggg aacaagcgaa tcagccttgt gcgcgaagcc gactgcacca tggtgacgtc
    11221 cagctggccg tcggtggggt ccgcgtcggg gcagatcagc atgccgccgc cgtagctgcg
    11281 cgtgttgccg aatgcggcaa gcgtcagttc ggtttcgagc tcacgcccgt caaacgtgag
    11341 gcgaaacggc aacagccgca acttggacag ctcagcgacc atcgcgacgt tgtaacgcat
    11401 ccggccgtgc ggccacgtca tccgattcgt ccggtcggtg
//
LOCUS       DASRJA010000315         4211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4142994,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000315 DASRJA010000000
VERSION     DASRJA010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4142994"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFC01_13660"
     CDS             <1..430
                     /locus_tag="VFC01_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN80713.1"
                     /translation="GQDFGKQKPSEVFRDHFLTCFISDHVGVQLRNDVGIDNICWEAD
                     YPHSDSMWPGAPEQLHDVLTDNSVPDDEVNKMTFENAMRWYHWDPFTHISREQATVGA
                     LRKAAEGHDVSIQALSKHEHGGANFADFAANAKELTGNKD"
     gene            534..1700
                     /locus_tag="VFC01_13665"
     CDS             534..1700
                     /locus_tag="VFC01_13665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80714.1"
                     /translation="MSFELTEDQELIRKSVAELAGKFDDQYWMEKDQAHEFPQEFYDA
                     IAKGGWLGMTIPEEYGGHGLGITEATLLLEEVARSGAAMNGASAIHLSIFGMQPVVKH
                     GSDELKARTLPRIVNGDLHVCFGVTEPGAGLDTSRITTFAKREGDHYRVNGRKVWISK
                     ALESEKILLLTRTTPYDEVAKKTDGMTLFLTDLDRDHVDIRPIKKMGRNAVSSNEVFI
                     DDLIVPIEDRVGEEGKGFKYILDGLNPERMLIAAEALGIGRVALEKAVKYGNERVVFN
                     RPIGMNQGLQFPLADSLARLDAAELVLRKATWLYDNGKPCGREANTAKYLCADAGFGA
                     ADRALQLHGGMGYSEEYHVSRYFRESRLMKIAPVSQEMILNFLGEHVLGMPRSY"
     gene            1701..2459
                     /locus_tag="VFC01_13670"
     CDS             1701..2459
                     /locus_tag="VFC01_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80715.1"
                     /translation="MTNYFDLTGRSALVTGAAGGIGSAVAAALADAGAAVLVTDLDKD
                     AAAAVSERISDAGGRAESAALDVSDRDSADTAAAQAAALADGALHIVINNAGVTKPAM
                     FEKTTQESFRLLFDIHVMGAFNVTQAALPYIPTDGTGRIVNVTSAAGLTGTLGQVNYS
                     AAKAGIIGFTKSLARELATKSIMVNALAPLAATPMTETIRTNEKFSANMMNRIPMKRW
                     AEPSEIAGAFVFMASDASSYITGQVLPVDGGMVM"
     gene            2459..3664
                     /locus_tag="VFC01_13675"
     CDS             2459..3664
                     /locus_tag="VFC01_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZN80716.1"
                     /translation="MAAGPLSGITVVTLEQAVSAPMCTRTLADFGARVIKVEHPNGGD
                     FARYYDDVVNGLAAHFVWCNRNKESITLDLKTTEGLDILHRLLDRADVLVSNLAPGST
                     GRLGITPADMEARHPNVIAVEIDGYGPGGPLSHKRAYDLLIQAESGTCAVTGEAGAPA
                     KPGPPVADITTGLQSALSIMALLYSRDTGRSAGGNSVAVSLFDTMMDVMGYQLTYTQH
                     SGIDQQPLGMSSPAVAPYGAYRTADDQTVVLGTTNDREWQRLAREILQRNDLADDERF
                     RTNADRVANRAVLDEAIGQWCAQHELDHVQKTADAAGIGNSRYNVPSEVVVHPQLTAR
                     DRWREVQTSAGPIQALVPPPVIAGYDPPMGAVPGLGQHTDAVLAELGLGEDEIAVLRD
                     RGVIGPAYD"
     gene            3664..>4211
                     /locus_tag="VFC01_13680"
     CDS             3664..>4211
                     /locus_tag="VFC01_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN80717.1"
                     /translation="MSGNEPVLLSSDHDGVRTLTLNRPDRKNAINPQLWIELADALRA
                     AARDDLRALVITGAGGAFCSGADISTPEDIHPRHKLRRLADVALALHELTIPTIAKVN
                     GVAVGAGWNLALGCDFVVATPESRFCQVFSKRGLSVDLGGSWLLPKLVGLQQAKRLVL
                     LAETIDADEARSLGLVTWVKPA"
BASE COUNT          795 a         1331 c         1394 g          691 t
ORIGIN      
        1 ggggcaggac ttcggtaagc agaagccctc agaggtgttc cgggatcact tcctgacctg
       61 cttcatctcc gatcacgtcg gggtgcagct gcgcaacgac gtcggcatcg acaacatctg
      121 ctgggaggcc gactacccgc acagcgacag catgtggccg ggtgccccgg agcagttgca
      181 cgacgtgctg acggacaaca gcgtgcccga tgacgaggtc aacaagatga ccttcgaaaa
      241 cgccatgcgc tggtatcact gggacccgtt cacccacatc agccgcgaac aagcgaccgt
      301 cggcgcgctc cgcaaggccg ccgaaggaca cgatgtctcc atccaggcgt tgtccaagca
      361 cgaacatggc ggcgcgaact tcgcggactt cgccgcgaat gccaaggagt tgaccggcaa
      421 taaggactga gtcgtaggtt ttaagtcaag tgcgccggtg cggatgagac cgtaaacgca
      481 ccggcgcact ggtttgtgca tcgaattaga ggagagtcat cgtgcccggc gggatgagtt
      541 tcgagctgac cgaggatcag gagctgatcc gcaagtccgt cgccgaactg gcgggcaagt
      601 tcgacgacca gtattggatg gagaaggacc aggcgcacga attcccccag gagttctacg
      661 acgcgatcgc caagggcggt tggctcggca tgaccatccc ggaggagtac ggcggccacg
      721 gtctcggcat caccgaagcg acccttctgc tggaggaggt cgcgcgctcc ggggcggcga
      781 tgaacggggc gagcgccatc cacttgtcga tcttcggcat gcagccggtg gtcaagcacg
      841 gctcggacga gctcaaggcg cgcacgctgc cccgcatcgt caacggtgat ctgcatgtgt
      901 gcttcggcgt gaccgaacct ggtgcgggcc tggatacttc gcgcatcacc accttcgcca
      961 agcgcgaggg cgatcactac cgcgtcaacg gccgaaaggt gtggatctcc aaagctctcg
     1021 agtccgagaa gatcctgctg ctcacccgca ccacccccta tgacgaggtc gccaagaaga
     1081 ccgacggcat gacgctgttc ctgaccgacc tggaccgaga ccacgtcgat atccgtccga
     1141 tcaagaagat gggccgcaac gccgtcagct ccaacgaggt tttcatcgac gatctgatcg
     1201 tgccgatcga ggacagggtc ggcgaggagg gcaagggctt caagtacatc ctcgatggac
     1261 tgaatcccga gcggatgctg atcgccgccg aggcactggg catcggccgg gtcgcgctgg
     1321 agaaggccgt caaatacgga aatgagcggg tcgtcttcaa ccgcccgatt gggatgaacc
     1381 agggtctgca gttcccgttg gcggattcgt tggcccgcct cgacgccgcc gaactggtgc
     1441 tgcgcaaggc gacctggctc tacgacaacg gcaaaccgtg tgggcgcgaa gcgaataccg
     1501 ccaaatacct ttgcgctgac gccggtttcg gtgccgctga ccgcgccctg cagctgcatg
     1561 gcgggatggg ctattccgag gagtaccacg tgtcgcggta cttccgcgag tcgcgcctga
     1621 tgaagatcgc gccggtcagt caggagatga tcctgaactt cctcggcgaa cacgtgctcg
     1681 gaatgcccag gagctactag atgaccaact atttcgacct gaccggccgg tcggcgctgg
     1741 tgaccggtgc cgcgggcggc atcggctccg cggttgccgc cgcgctggca gatgcgggcg
     1801 ccgccgtgct cgtcaccgat ctcgacaagg atgcggcagc cgctgtgtcc gaacgtattt
     1861 cggatgccgg cggtcgggcc gagtccgccg cactcgatgt gtcggaccgg gactccgccg
     1921 acacggcggc ggctcaggcg gcagcactgg ccgacggtgc gctacacatc gtgatcaaca
     1981 acgcgggtgt caccaagccc gcgatgttcg agaagaccac gcaggagtcg tttcggctgc
     2041 tgttcgacat ccatgtgatg ggcgcgttca acgtcaccca ggcggcgctg ccgtacatcc
     2101 cgaccgacgg caccggacgc atcgtcaacg tcacgtcggc ggccgggctc acgggaaccc
     2161 tcgggcaggt caactactca gcggccaagg ccggcatcat cggcttcacc aagtcgcttg
     2221 cgcgcgaact ggccaccaag agcatcatgg tcaacgcatt ggctccgctg gcggcgacgc
     2281 cgatgaccga aaccatccgg accaacgaga agttctcggc caacatgatg aaccgcattc
     2341 cgatgaagcg gtgggcggag ccgtcggaga tcgcaggcgc gttcgtgttt atggcatcgg
     2401 acgcctcgtc gtacatcacc gggcaggtgc tgccagtcga cggcggaatg gtgatgtgat
     2461 ggcagcagga ccgctgtccg gcatcaccgt cgtcaccctg gagcaggcgg tgtcggcgcc
     2521 gatgtgcacc cgaacgctgg cggacttcgg cgccagggtc atcaaagtcg agcatccgaa
     2581 cggcggagac ttcgcccgct actacgacga cgtcgtgaat ggccttgcgg cgcacttcgt
     2641 ctggtgcaac cgcaacaaag agtccatcac cctagacctg aaaaccactg agggactcga
     2701 tattctgcac cgcctgctcg accgcgccga cgtgctggtg tcgaacctgg caccagggtc
     2761 gacgggccgg ctgggcatca cccccgccga catggaggcc cgccacccga acgtgatcgc
     2821 ggtggagatc gacggctatg ggccgggcgg gccgctgtcg cacaagcggg cctacgatct
     2881 gctgatccaa gccgaatcgg gcacgtgtgc ggtcacgggc gaggccggcg cacctgcgaa
     2941 gcccggcccg cccgtcgccg atatcacgac cgggctgcag tcggcgctct cgatcatggc
     3001 gctgctgtat tcgcgtgaca ccggtcggag cgcgggtgga aacagcgtag ccgtcagcct
     3061 gttcgacacc atgatggacg tgatgggcta ccagctcacc tacacgcagc actcgggaat
     3121 tgaccagcag ccgctgggca tgagctctcc ggctgtggca ccgtacgggg cgtatcgcac
     3181 cgccgacgac cagaccgtgg tgctgggcac gaccaacgat cgcgaatggc agcgactcgc
     3241 acgagaaatc ctgcagcgca acgacttggc cgatgatgag cggttcagga cgaacgcgga
     3301 ccgggtggcg aaccgggctg tgctcgatga ggcgatcggc caatggtgtg cccaacacga
     3361 gctggatcac gtgcagaaga cggcggacgc cgccggaata ggcaactccc ggtacaacgt
     3421 gcccagcgag gtggtcgtac acccgcagtt gaccgcacgc gatcgctggc gtgaggtcca
     3481 gacgtcggcg gggccgatcc aggcgctggt gccgccccct gtcatcgccg gatacgatcc
     3541 cccgatgggg gcggttcccg ggctgggcca gcacaccgac gccgtactcg ccgagctcgg
     3601 tttgggggaa gacgagatcg ccgtgctgcg cgatcggggc gtgatcggtc cggcgtacga
     3661 ctgatgtctg gcaacgagcc cgtattgctg tcgagcgacc acgacggcgt caggacgctg
     3721 acgctgaatc ggcccgatcg caagaatgcg atcaatccgc agctatggat cgagctggcc
     3781 gacgcgctgc gggccgccgc tcgcgacgac ctgcgtgcct tggtgatcac cggggcgggc
     3841 ggtgcatttt gttcaggagc cgacatctcc actcccgagg acattcaccc ccggcacaag
     3901 ctgcgcaggc tcgccgacgt cgcgctggca ttgcatgagc tgaccatccc caccatcgcc
     3961 aaggtgaacg gcgttgccgt gggtgcgggg tggaatctcg ccctgggatg cgatttcgtg
     4021 gtcgcgacgc ccgaatcgcg tttctgccaa gtcttttcga agcgcggact gtcggtggac
     4081 ctgggcggct cgtggctgct tcctaaactg gtcggcctgc agcaggccaa acgccttgtg
     4141 cttctcgccg agacgatcga cgccgacgag gcacggtcgc tggggttggt cacgtgggtg
     4201 aagccggccg a
//
LOCUS       DASRJA010000316         8743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2774806,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000316 DASRJA010000000
VERSION     DASRJA010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2774806"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VFC01_13685"
     CDS             complement(<1..846)
                     /locus_tag="VFC01_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003413944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HZN80718.1"
                     /translation="MAATKARPATDEPVKRTATKTPAKKTAASRASGSAAAKSANGSA
                     PAKRATKTTAASRESGSAAKPAKATAAKAAKAAPAKSRPSTRGAKAKADTPAPRGRAK
                     KATAPEAGVVDEDITDEVETDDDLEAEPEVLDEDEADLDLEDLEVGDDDTAAEAPDEE
                     AEGDAGAEPAAKSAEGVPPAEDDEIAEPSEKDKASGDFVWDEEESEALRQARKDAELT
                     ASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLYATQLMLELTEKGEKLPAAQRRD
                     MSWICR"
     gene            complement(1030..1839)
                     /locus_tag="VFC01_13690"
     CDS             complement(1030..1839)
                     /locus_tag="VFC01_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZN80719.1"
                     /translation="MTATDSPAADPATNGGPQRRGFGIDVGGSGVKGGIVDLDTGHLI
                     GERFKLETPQPATPDAVAKTVAAVVKEFGWTDRLGVTYPGVVTNGIVQTAANVDKGWI
                     GVNARDVIGAALGGQPVTVLNDADAAGLAEEKFGAGRDNTGVIVLLTFGTGIGSAVIH
                     NGVLLPNTEFGHLEVGGKEAEHRAASSIKEKKGWSYERWTQEVTRVLVAVENAIWPDL
                     FIAGGGISRKADKWIPLLKNRTPVVAAALQNTAGIVGAAMAAEVDVTATAK"
     gene            1953..2789
                     /locus_tag="VFC01_13695"
     CDS             1953..2789
                     /locus_tag="VFC01_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZN80720.1"
                     /translation="MAENDDDPVLLRSVAEGLATEAAEFVRRRRVEVFGNPQPGAAPP
                     VRAKSTPTDPVTVVDTETERLLRDRLAELRPGEHILGEEEGGSTGGREGLTWVLDPID
                     GTVNFVYGIEAYAVSVAVQRGGVSVAGAVADVPAAAVYSAALGDGARVKRDGAVTPLG
                     SSAAADLSMSLVGTGFAYAPEQRARQAEILARMLPVVRDVRRIGSAALDLCMVAAGRL
                     DAYYEDGVHVWDCAAGALIAAEAGAILRLPPVDREPGFIVAAAPGIADALAGALRSSG
                     AL"
     gene            complement(2824..3480)
                     /gene="cei"
                     /locus_tag="VFC01_13700"
     CDS             complement(2824..3480)
                     /gene="cei"
                     /locus_tag="VFC01_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope integrity protein Cei"
                     /protein_id="HZN80721.1"
                     /translation="MVAQITEGTAFDKRGRPFRRRNFLPGILLFVALAVVTLLVWVMA
                     LSQPADIHEATVCNAPPPPADPSAPAAQLGEQVTRADMTDVTPAKLADAKIRVLNASG
                     QGGQAAEVAGELRDLGFAEPAAANDTMYVNSRLECQGQIRFGPSGRAAAAAVWLVAPC
                     TELFQDQRADDTVDLAIGTEFTELTHSDDIDAVLASLRPDATQPADPTLLKKIHTAGC
                     "
     gene            3647..3949
                     /locus_tag="VFC01_13705"
     CDS             3647..3949
                     /locus_tag="VFC01_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HZN80722.1"
                     /translation="MATDYDAPRRTETDDVSEDSLEELKARRNEAQSAVVDVDESESA
                     ESFELPGADLSGEELSVRVIPKQADEFTCSSCFLVHHRSRLASEKNGVMICTDCAA"
     gene            complement(3969..4445)
                     /locus_tag="VFC01_13710"
     CDS             complement(3969..4445)
                     /locus_tag="VFC01_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 domain-containing protein"
                     /protein_id="HZN80723.1"
                     /translation="MSDTRATSQSVRYRERLRVPWWWWPPGLGLAGLIALEVNQGVPA
                     IPNWVPFAVLLPVAAVVLLWFGKTEVSVVGGSGETELWVGAAHLPVSVIARSAEVPKS
                     AKSAALGRQLDPAAYVVHRAWVGPMVLLVLDDPDDPTPYWLVSCRHPDRVLAALRG"
     gene            4471..4935
                     /gene="dut"
                     /locus_tag="VFC01_13715"
     CDS             4471..4935
                     /gene="dut"
                     /locus_tag="VFC01_13715"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZN80724.1"
                     /translation="MSTSLAVVRLDRDLPMPSRAHDGDAGVDLFSALDVELAPGHRAL
                     VPTGVAVAIPHGMVGLVHPRSGLAARVGLSIVNSPGTIDAGYRGEIKVSLINLDPDTP
                     IVVHRGDRIAQLLVQRVELPELVEVTSFDEAGLADTTRGDGGHGSSGGHASL"
     gene            4935..5681
                     /locus_tag="VFC01_13720"
     CDS             4935..5681
                     /locus_tag="VFC01_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HZN80725.1"
                     /translation="MAFGKRKDSDDVDGPADLTGDPVDEELEGPFDIDDFDDAATAAQ
                     GRLDLGSVLIPMPEAGQVQVELTEVGVPSAVWVVTPNGRFTIAAYAAPKTAGLWREVA
                     TELADSLRKDVPKVSIEDGPWGREVVGVSKAEQNQQAGVVRFIGVDGYRWMIRCVVNG
                     PQDKIAPLADEARNALADTVIRRGDTPLPVRTPLPVQLPEPMAAQLRAAAEQAAQQQA
                     QQPQEQPPQPVARRSEQGSAMQQLRTITGG"
     gene            complement(5678..6379)
                     /locus_tag="VFC01_13725"
     CDS             complement(5678..6379)
                     /locus_tag="VFC01_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN80726.1"
                     /translation="MTVNLRGVTTVLLPGTGSDDDYVYRAFSDALHGVGAVVVTPRPR
                     PDRLIDGYRDALDDAARAAAPIAVGGVSIGAAVAAAWALAHPGHTVAVLAALPAWTGD
                     PDTAPASLAARHSAEMLRRDGLVSATAQMRASSPPWLADELTRSWVGQWPSLPDAMDE
                     AARYVAPTCGQLEGLAVPMGVAAATDDPVHPVEVGIEWVAAAPRAALRTVTLDEMGAN
                     PAVLGAACLAALTDA"
     gene            6513..6884
                     /locus_tag="VFC01_13730"
     CDS             6513..6884
                     /locus_tag="VFC01_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HZN80727.1"
                     /translation="MATAEGYLRRLTRRLTEDPEQLDAEELTDEAANTGAQKAIDCQR
                     GQEVTMIGTLRSVECNGKGCSGGVKAELFDGTDSVMLVWLGQRRIPGIESGRTLRVHG
                     RIGKLDNGAKAMYNPHYEIQK"
     gene            6881..7585
                     /locus_tag="VFC01_13735"
     CDS             6881..7585
                     /locus_tag="VFC01_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HZN80728.1"
                     /translation="MSDPGPEPGKQHKANAVLEQMGGISGLIYSSLPVVAFVPMSTLF
                     GLVPAIGAALGVAAVILAWRLIRRESVQPAISGFIGVGISALIAYMVGESKGYFLLGI
                     WMSLFWAVVFTASILIRRPVVGYIWGWVNSHDRDWRGLRKAVVAFDLATFVWVLVFTS
                     RFVVQQHLYDADQTGWLGVARIAMGWPLTAVAALVTYLAIRAAQRALHAHDSEKAEET
                     GETAEEHGGVAQPDAH"
     gene            7575..>8743
                     /locus_tag="VFC01_13740"
     CDS             7575..>8743
                     /locus_tag="VFC01_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN80729.1"
                     /translation="MPTETDTDRPLIAVAVLASFVAFLDGSVVNLALPAIGREFGGGL
                     ALQQWVVDGYLLTLGALILVAGAISDQFGRLAVLRMGIVIFGVASLLCALAPSGWILV
                     AARCLQGVGAAFLVPSSLAMINSHFAGPAQARAIGTWTAWTGTAFVIGPLLGGVLVDA
                     VGWRWIFGVNIVPLAVTLFLATKLSADEFAGDRSARIDVVGAVLNAVGLTGAVYALIE
                     GQRLGFSYPPVAVSLVVGLACLVVFPWWERRTPHPMMPPHIFAARNFAVGNLATVFLY
                     AAVSLGTLIVALFLQESAGLSATEAGLATLPVPVLSFFLARRFGTLAGVHGPRLFMAV
                     GPVVAAAGYLWMMMAREPFDFWTQMLPGLVVFGLGLSITVSPLTAAILAAVDPAQ"
BASE COUNT         1291 a         2921 c         3024 g         1507 t
ORIGIN      
        1 gcggcagatc cacgacatgt cgcgacgttg cgcggcgggc agcttctcgc ccttctcggt
       61 caattcgagc atcagctgcg tggcgtagag gccggcctcg atccgcttgg ccagctcgac
      121 ctcttcctcc gcgttgagca gtgcgacctt gccgatctgc ttgaggtagg cgcgcaccga
      181 gtccgccgac gcggtgagct ccgcgtcctt gcgggcctgc cgcagcgctt cggattcctc
      241 ttcgtcccaa acgaagtcgc cggaagcctt gtccttctcg gacggttccg cgatctcgtc
      301 gtcctcggct gggggcacgc cttcggcgga cttcgccgcg ggttcggccc cggcatcgcc
      361 ctccgcctcc tcgtcgggag cttccgcggc ggtgtcgtcg tcgccgacct cgaggtcctc
      421 aaggtcgaga tcggcctcgt cttcgtcgag gacctcgggc tcggcctcga gatcgtcgtc
      481 cgtctcgacc tcgtcagtga tgtcctcgtc gacgacgccg gcttcgggcg cggtcgcctt
      541 cttcgcgcga ccgcggggtg ccggcgtgtc ggcctttgcc ttcgcgcccc gcgtcgacgg
      601 ccgcgacttg gccggggcgg ccttcgcggc cttggccgcg gtggccttcg cgggcttggc
      661 cgcgctgcct gattcgcggc tcgccgccgt cgtcttggtg gcccgcttcg cgggcgccga
      721 accattggct gactttgccg ccgcgctacc tgatgcgcgg ctcgccgcgg tcttcttggc
      781 gggggtcttg gtagcggtgc gcttcaccgg ctcatcggtt gccgggcgtg cctttgtcgc
      841 tgccacgtac accctttcga tcttgcaaat tccgacgggc acggtcgtgc accatcgaaa
      901 agcgtcggct atgtcgaata ttggcggttg atctcgccgc tctccgggat gcggccgccg
      961 tcgaccattg taacgacagt gtgaggtacc gccgtgttca gcggcaaatt tgacgcgcgg
     1021 ccgcgttggc tacttggcag tagccgtgac atcgacctcg gcggccatcg ccgcgccgac
     1081 gatgcctgcc gtgttttgca gcgctgcggc caccaccggc gtgcggttct tcagcagcgg
     1141 aatccattta tccgctttgc ggctgattcc gccgcccgca atgaacagat ccggccagat
     1201 cgcgttctcg accgccacca gcaccctggt gacctcttgc gtccagcgct cgtagctcca
     1261 gccctttttc tccttgatgg acgacgccgc ccggtgttcg gcctctttac cgccgacctc
     1321 caggtggccg aactcggtgt tgggcagcaa gaccccgttg tgaatcaccg ccgagccgat
     1381 gccggtacca aacgtcaaca gcacgatgac cccggtgttg tccctaccgg cgccgaactt
     1441 ctcctcggcc agtccggcgg cgtcggcgtc gttgagcacg gtcacgggct ggccgcccag
     1501 cgcagcgccg atgacgtcgc gggcattgac gccgatccat cccttgtcga cgttggccgc
     1561 cgtctgaacg atcccgttgg tcaccacgcc ggggtaggtg acgccgagtc tgtcggtcca
     1621 gccgaattcc ttgacgacgg ccgcgacggt cttcgcgacg gcgtcgggag tggcgggttg
     1681 cggggtctcg agcttgaagc gctcgccgat caggtgcccg gtgtccaggt cgacgatgcc
     1741 gcccttgacg ccgcttccgc cgacgtcgat gccgaatccg cggcgttgcg ggccaccgtt
     1801 ggtggcgggg tcggcggcgg gtgaatcggt ggcggtcatg agcgctcctc acagaggccg
     1861 gcaggtgcga cgcgccccac gttagtagct cgggaaatct cacgcgtggc tgtcgcgcaa
     1921 ctgcatagcg ccatctgtgc tgcgatagac ccgtggcgga aaacgacgac gatcccgtcc
     1981 tgttgcgctc ggtcgcggag gggttggcga cggaggccgc cgagttcgtc cggcgccgcc
     2041 gcgtcgaggt tttcgggaat ccccaaccag gcgccgcccc accggtgcgg gcaaagagca
     2101 caccgaccga tcccgtcacc gtcgtcgaca ccgaaaccga gcggctgttg cgcgaccggc
     2161 tggccgaact gcggccgggt gaacacatcc tcggcgagga ggaaggcggg tcgaccggcg
     2221 gccgcgaggg cctgacgtgg gtgctcgacc cgatcgacgg cacggtcaac ttcgtctacg
     2281 ggatcgaggc atacgcggtg tcggtggcgg ttcagcgcgg cggtgtgtca gtcgccggag
     2341 cggtggcaga cgtgcccgcc gcagcggtgt actccgccgc actgggtgat ggcgctcgcg
     2401 tgaagcggga cggcgccgta acaccactcg gctccagtgc ggccgccgac ctgtcaatgt
     2461 cgttggtggg caccggcttt gcctatgcgc ccgagcaacg ggcacggcag gccgagatcc
     2521 tcgcaaggat gcttcccgtg gtgcgcgatg tgcgccggat cggctcggcc gcgctcgatc
     2581 tgtgcatggt ggccgctggg cggctcgacg cctactacga ggacggcgta cacgtctggg
     2641 actgcgcggc cggtgcgttg attgccgccg aggcgggcgc gatcctgcga ctgccgcctg
     2701 tcgacaggga accaggattc atcgtggcgg ccgcgccggg catcgccgac gcactagccg
     2761 gcgcgttgcg cagttcgggt gcgttgtagc cccgaatgcc ggttgcccca acgcaattca
     2821 ggcctagcag ccggcggtgt ggatcttctt gagcagcgtc ggatcggcgg gctgggtggc
     2881 gtcggggcgc aaactggcca gcaccgcgtc gatgtcgtcg ctatgtgtca actcggtgaa
     2941 ctccgtaccg atggcgaggt cgacggtgtc gtcggcgcgc tggtcctgaa acagctcggt
     3001 gcatggcgcg accagccaca ccgccgccgc ggcggcgcgc ccggacggac cgaaccggat
     3061 ctgaccctgg cattccagcc gggagttgac gtacatcgtg tcgttggccg cggccggctc
     3121 ggcgaacccg aggtctcgca gttcaccggc cacctcggcg gcctgcccgc cctggccgct
     3181 ggcgttgagg acccggatct ttgcgtcggc gagcttggcc ggggtgacgt cggtcatgtc
     3241 ggcgcgggtc acctgctcac cgagctgcgc agccggggcg ctcggatcgg ccggaggtgg
     3301 cggcgcgttg cacacggtgg cctcgtggat gtcagcaggt tgactcaggg ccatcaccca
     3361 caccaacagc gtcaccaccg cgagggcgac gaacagcagg atcccgggca ggaagttgcg
     3421 ccggcgaaag ggccgacccc gcttgtcgaa cgcggtgcct tctgtgattt gcgcgaccac
     3481 gagtgcactc taaggccaat gacgctgggc ccttcctgcg gtgggaccta cactgtgacg
     3541 taaatcacag tggaacgcgt gtcaatacgg gcacgaaacg tttggggaat gcgttcgacg
     3601 ctggtacaaa gctctgctgc aaggttttac ggaggggaca agaggaatgg ctacggatta
     3661 cgacgcccca cggcgcacag agactgatga cgtttccgag gattcgctcg aggagctcaa
     3721 ggcgcggcgt aacgaagccc agtcggcggt cgttgacgtc gatgaatcgg agtctgccga
     3781 gtccttcgaa ctgcccggcg ccgacctgtc cggcgaggaa ctgtctgtgc gggtgatccc
     3841 gaagcaggcc gacgagttca cgtgctcaag ttgctttctg gtccatcacc gcagccggct
     3901 ggccagcgag aagaacggcg tgatgatctg caccgactgc gcggcttgag tctctggaat
     3961 ctcaggaatc aaccgcgaag ggccgctagc acccgatcgg gatggcggca gctcaccagc
     4021 cagtacggcg tcggatcgtc gggatcgtca agaaccagaa gaaccatcgg acctacccat
     4081 gcccgatgca cgacgtaggc cgccggatcg agctggcggc ccagcgcggc cgatttggcc
     4141 gatttgggca cctcggccga ccgtgcaatc acgctgaccg gcagatgggc cgccccgacc
     4201 cacagctcgg tctcgccgga accgccgacg acactcacct ccgtcttgcc gaaccacagc
     4261 agcacgacgg cggcgacagg cagcaaaacc gcaaacggca cccaattcgg gatcgcgggc
     4321 acgccctgat tcacctcgag cgcgatcagc ccggccaacc ccagcccggg cggccaccac
     4381 caccaaggca cccgcaaccg ttcgcgatag cgcacagatt gcgacgtggc gcgcgtgtcg
     4441 gacacgcggc caagagtaat ctctgacgtc gtgtccactt ctctggcggt cgtgcgcctt
     4501 gatcgcgatt tgccgatgcc cagccgtgct cacgacggcg atgcgggcgt cgaccttttc
     4561 agcgcgctcg acgtcgagct ggcaccaggt caccgggcgc ttgtgccgac cggtgtcgcc
     4621 gtcgccattc cccacggcat ggtcgggcta gtgcaccctc gctcgggttt ggctgcgcgc
     4681 gtggggcttt cgatcgtcaa cagccccggc acgatcgacg ccggttaccg tggggagatc
     4741 aaggtatcgc tgatcaacct cgaccccgac acgccgatcg tcgtgcaccg cggcgaccgg
     4801 atcgcccagt tgttggtgca acgggtcgag ctgccggagc tggtcgaggt gacgtcgttc
     4861 gatgaggcag gcctggcgga caccacccgt ggcgacggtg gccacggttc ctctggcgga
     4921 catgcgagtt tgtgatggca ttcggaaagc gcaaagacag cgatgacgtg gacggcccag
     4981 ccgatctgac gggcgacccc gtcgacgagg aactcgaagg ccccttcgac atcgacgact
     5041 tcgacgacgc ggcgacggcg gcgcagggcc ggctggatct cggctcagtg ctgattccga
     5101 tgcccgaggc cggccaggtg caggtcgagc tcaccgaggt gggggtgccc agcgccgtct
     5161 gggtggtcac acccaacggg cgcttcacga tcgccgccta tgccgccccg aagaccgctg
     5221 gcctgtggcg ggaggtcgca accgagctgg ccgactcgtt gcgcaaagac gtgcccaagg
     5281 tcagcatcga ggacgggccg tggggtcgcg aagtggtcgg cgtctcgaag gctgagcaga
     5341 accagcaggc cggggtggtc cggttcatcg gcgtggacgg ctaccgctgg atgatccgat
     5401 gtgtggtcaa cggcccacag gacaagatcg cgccgctggc cgacgaagcg cggaatgcgt
     5461 tggcggacac cgtcattcgg cgtggcgata cgccgctgcc ggtccgcacg ccgctgccgg
     5521 tgcaactgcc cgagccgatg gcggcgcagc tgcgcgcggc ggccgaacaa gccgctcagc
     5581 agcaggcgca gcagccccaa gagcagccgc cccagcctgt cgcccgtcgg agcgagcagg
     5641 ggtcggccat gcagcagctg cggaccatca ccggcggcta ggcgtccgtc agcgcggcca
     5701 ggcaggcggc gccgagaacg gccgggtttg cgcccatctc gtcgagcgtg accgtgcgca
     5761 gcgccgcgcg cggtgcggcc gccacccatt cgatgccgac ctcgaccgga tgcaccgggt
     5821 cgtcggtggc ggcggccacg cccatcggca cggccagacc ctccagctga ccgcaggtcg
     5881 gcgcgacata acgggcggcc tcgtccatcg cgtcgggcag cgacggccac tggccgaccc
     5941 aggaccgcgt cagctcgtcg gccagccagg gcgggctgga ggcgcgcatc tgcgccgtcg
     6001 ccgacaccag gccgtcgcgc cgcagcatct ccgccgagtg ccgcgccgcc agggaggccg
     6061 gtgcagtatc cgggtcgccc gtccaggccg gcagggcagc gagtacggcg accgtgtggc
     6121 cggggtgggc cagcgcccac gcggcggcca ccgcggcgcc gatggacacc cctccgacgg
     6181 cgatcggggc ggcggcccgc gcggcgtcat cgagcgcatc gcggtagccg tcgatgagcc
     6241 gatccggcct cgggcgcggt gtcactacga ccgccccgac accgtgcagg gcgtcggaaa
     6301 aggcccggta gacgtagtcg tcatcggaac cggtgccggg cagcagaacc gttgtgaccc
     6361 cgcgcagatt gaccgtcatc acaagatcgt cccccgttca ccgactggtc ggcgtggcga
     6421 cccagccccg cgtggcatat cggaaccaag aggtctaccg tggcgttgga ttgggtagat
     6481 ccatggtgga tctgcagaag tcaggagagg ccatggctac ggccgaaggg tatctgcgcc
     6541 ggctcacccg ccggttgacg gaggacccag agcaactcga cgccgaagag ctcaccgacg
     6601 aagccgccaa caccggcgcc caaaaggcga tcgactgtca acgcggacag gaagtgacga
     6661 tgatcggcac gctgcgcagc gttgaatgca acggcaaggg ctgttccggt ggcgtgaaag
     6721 ccgaactctt cgacggcacc gactcggtga tgctggtctg gctcggccag cgccgcatcc
     6781 cgggtatcga gtccggccgc acgctgcggg tgcacggccg catcggcaag ctcgacaatg
     6841 gcgccaaggc gatgtacaac ccgcactacg agattcaaaa gtgagcgacc ccggacctga
     6901 accggggaag cagcacaaag cgaacgccgt cctcgagcag atgggcggca tcagcggcct
     6961 gatttactct tcgctgcccg ttgtggcgtt cgttccgatg tccacccttt tcggcctggt
     7021 accagccatc ggcgcggcgc tgggcgtggc cgcggtgatc ctggcgtggc ggctgatccg
     7081 ccgcgagtct gtccagccgg cgatctccgg gttcatcggc gtgggaatca gcgctctgat
     7141 cgcctacatg gtcggcgaat cgaagggcta tttcctgctc ggcatctgga tgtcgttgtt
     7201 ctgggcggtc gttttcacgg catcgatcct gattcgccgt cccgtcgtcg gctacatctg
     7261 gggctgggtc aattcccacg accgcgactg gcgtggactg cgcaaggccg tggtggcgtt
     7321 cgacctcgcg acgttcgtgt gggtgttggt gttcacctcg cgattcgttg tgcagcagca
     7381 tctctacgac gccgaccaga caggctggtt gggcgttgcc cgcatcgcga tgggctggcc
     7441 gctgaccgcg gtggccgcgc tcgtgacgta tctggccatt cgggccgccc agcgagcgct
     7501 gcacgcacat gattccgaaa aggccgagga gacgggggag accgccgagg aacacggagg
     7561 cgtcgctcaa cccgatgccc actgagaccg acaccgaccg gccgctgatc gcggtcgcgg
     7621 tgctggcctc gttcgtcgca ttcctcgacg ggtcggtggt caacctggcg ctgcccgcca
     7681 tcggccggga attcggcggc ggcctggcgc tgcagcagtg ggtcgtcgac ggatatctac
     7741 tcacgctcgg agcgctgatc cttgtcgcag gcgcgatttc cgatcagttc ggccgactgg
     7801 ccgtgctgcg gatgggcatc gtcattttcg gggtggcatc actgctgtgc gcgctggcgc
     7861 ccagcggctg gatcctggtg gccgcccgct gcctgcaggg cgtcggtgcg gcgtttctgg
     7921 tgcccagctc tctggcgatg atcaactccc atttcgccgg ccccgctcag gcgcgtgcga
     7981 tcggcacgtg gacggcatgg accggcacgg cattcgtgat cggcccgctg cttggcggcg
     8041 tgttggtcga cgcggtgggc tggcggtgga tcttcggcgt caacatcgtt ccattggccg
     8101 tcacgctgtt cctggcgacc aagctgtcgg ccgacgagtt cgccggtgac cggtcggccc
     8161 gcattgacgt cgtcggcgcc gtgctgaacg ccgtcgggct gacgggcgcg gtgtacgcgt
     8221 tgatcgaagg gcagcggctc ggcttctcgt atccgccggt cgcggtgagc ctggtcgtcg
     8281 gcctggcgtg tctggtcgtg ttcccgtggt gggagcgccg cacgccgcat ccgatgatgc
     8341 cgccgcacat cttcgccgcc cgcaacttcg cggtgggcaa cctcgcaacg gtgttcctct
     8401 atgcggccgt ctcgctgggc acgttgatcg tcgcgctgtt cctgcaggag tccgcgggat
     8461 tgtcggccac cgaggcaggg ctcgccacgc ttccggtgcc tgtgctgtcg ttcttcttgg
     8521 cccgccggtt cggcacgctg gccggcgtgc acgggccacg cctgttcatg gccgtcggtc
     8581 cggttgtcgc cgcagccggc tacctctgga tgatgatggc gcgcgagcca ttcgacttct
     8641 ggactcaaat gctgccaggg ctggtggtgt tcgggctcgg gctctcgatc accgtgtcgc
     8701 cgctgacggc ggcgatactg gccgcggtcg atcctgccca gag
//
LOCUS       DASRJA010000317         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_318196,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000317 DASRJA010000000
VERSION     DASRJA010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318196"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..596)
                     /locus_tag="VFC01_13745"
     CDS             complement(156..596)
                     /locus_tag="VFC01_13745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HZN80730.1"
                     /translation="MATTRTDPYFGYNFAVELDGITSMGFKTCAGLDTSTTSTKYREG
                     TDVSLAQRELPALLHHTAITLTRGISADRALFDWRNDIASGRPNRRDISIVLRDDLGV
                     ERVRWTVKNAWPSKWSGPSFDAASDAIAIETLELTHEGVVVQKW"
     gene            complement(623..1888)
                     /locus_tag="VFC01_13750"
     CDS             complement(623..1888)
                     /locus_tag="VFC01_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80731.1"
                     /translation="MASLNRPGVVVESFPPSVSAAFRTGVAGFVGPAVAGSSPVPPGP
                     ALPSLPQTLLFTSWPHFADTFRDSKGWLPGIWLPGELLWAAVQGFFANGGSRCWVVLY
                     DKNASADPLAAVDIGVTALASIDDVDLMCAPSLLDSDSLTAQQQLRLQQQLIWSAENR
                     GRDDWFLILDAPRPPAGTLTVEAQLAAQLPGLRDDPTFTTLGGAAYHMRRPNNAAVYY
                     PWLVPGGDVEPAPGAVATSIPASGHVAGTFARTDLQVGVHKAPANEPIDGIVNVLADV
                     DDVATANPIRAYPGRGIRVWGARTLAVVASPGDLNAYVGVRRLMVTLDRWLVRAFEPM
                     VFEANEVRTWVRIHRELNRRLTAMFEQGAFQGSTPDEAFYVKCDDENNPVEARAAGRL
                     QVDIGVAPAVPKEFITIRLVRSAEGITVA"
     gene            complement(1894..3063)
                     /locus_tag="VFC01_13755"
     CDS             complement(1894..3063)
                     /locus_tag="VFC01_13755"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HZN80732.1"
                     /translation="MPTYTAPGVFVEEVPATAPIVGVGTSTPVFIGVWADGASDPNPS
                     GTVIPIFNITKFKTAFGDFTAPPGTGQTLNAGLNTLGHAVKGFFENGGTFCYAVRAKV
                     ETDIGAVVLPKLEAFDDISMVVAPGVPAATASMTAVTDIVTHCRNMGDRIAILDAPAN
                     ATSPTQLAPLLPTGGSAAYQATDFAALYTPWLVVFDDATKGEKTVPPSGHIAGIYSRV
                     DATRGVHKAPANEQLFGVLRPSVQIGKTGQGTINPQGGNCIRMLNGAIKVWGARTLAG
                     DTSLHKYVPIRRLLSFLYKSIDHGTQWAVFEPNGPDLWARIIRNVGSFLTNVWSQGAL
                     AGAVPEEAFYIKCDDENNPPDQRASGLLIVEVGVATVFPAEFVVFRIQQFTGPIR"
BASE COUNT          524 a         1057 c         1100 g          543 t
ORIGIN      
        1 gaggtagccc gggttcgact ggacgcgggg gtccttgagc ggcgcaatct cgtcgtacgc
       61 cgtcgcgagg cgcatgaccc cctcgcgatg attgacgcct tcctggtcgc gatagcctcg
      121 cggcagcacg aacgggatct cgccgtttgc cgggtctacc atttctgcac cacgacgcct
      181 tcgtgcgtca gctcgagcgt ctcgatcgcg atcgcatccg acgcggcgtc gaagcttggc
      241 ccgctccact tggacggcca cgcgttcttg accgtccagc ggacccgctc gaccccgagg
      301 tcgtcgcgca ggacgatcga gatgtcgcgt cgattcggcc ggccgctcgc gatgtcgttg
      361 cgccagtcga acagcgcgcg gtccgcggaa atcccgcggg tcagcgtgat cgcggtgtgg
      421 tgaagcagcg ccggcagctc ccgttgcgcg agcgagacat cggtgccttc gcggtacttc
      481 gtcgacgtcg tgctggtatc cagcccggcg caggtcttga agcccatgct ggtgatgccg
      541 tcgagctcga cggcgaagtt gtaaccaaaa tacgggtcag tacgtgtcgt agccatcgtg
      601 cgctagctcc tcggtgtgac ggtcacgcaa cggtgatgcc ttccgcgctg cggaccaggc
      661 ggatcgtgat gaactccttc gggacggcgg gcgcgacgcc gatatcgacc tggagccgcc
      721 cggccgcgcg cgcctcgact ggattgtttt cgtcgtcgca cttgacgtag aacgcctcgt
      781 cgggcgtgct gccctggaac gcgccctgct cgaacatggc ggtgagtcgc cggttcagct
      841 cgcggtggat ccgcacccag gtgcgaacct cgttggcctc gaacaccatc ggttcgaacg
      901 cgcgaaccag ccagcggtcg agcgtgacca tcagccggcg aacgccgacg taggcgttaa
      961 gatcccccgg tgaggccacg accgcgagcg tgcgcgcgcc ccaaacccgg ataccgcgcc
     1021 ctggatatgc gcggatcgga ttggcggtgg cgacgtcatc gacgtcggcc agcacgttga
     1081 cgatgccgtc gatcggttcg ttggccggcg ccttgtgcac gccgacctgg aggtcggtgc
     1141 gcgcgaaggt ccctgcgacg tggccgctcg ccgggatcga agtggcgacc gcacccggcg
     1201 cgggctcgac gtcgcctccc gggaccagcc aggggtagta gaccgcggca ttgttcggcc
     1261 gccgcatatg atacgcggcg ccgccgagcg tggtgaacgt cgggtcgtcg cgcaaaccgg
     1321 ggagctgcgc ggcgagctgg gcttccacgg tcagcgtgcc ggccggcggc cgcggcgcat
     1381 cgaggatcag aaaccagtcg tcgcgcccgc ggttctcggc gctccagatc agctgttgct
     1441 gcagccggag ctgctgttgc gcggtgagcg aatccgagtc aagcagcgat ggcgcacaca
     1501 tcaggtccac gtcatcgatc gaagccagtg cggtcacgcc gatatcaacg gcggcgagcg
     1561 gatcagcgct cgcattcttg tcgtacagca cgacccagca ccggcttccg ccgttggcga
     1621 agaagccttg gactgccgcc cacagcagtt cgccggggag ccagatgccg gggagccagc
     1681 ccttgctgtc gcggaaggtg tcggcgaagt ggggccagct ggtgaacagc agggtctgcg
     1741 gcagcgacgg cagcgccggg ccgggaggca cgggcgacga cccggcaacc gccggaccga
     1801 cgaacccggc gacgccggtg cgaaacgcgg cgctcacgga tggtggaaac gactccacca
     1861 ccacccccgg gcgattcagc gacgccatgg cgactacctg attggaccag tgaactgctg
     1921 aatacggaac acgacgaact cggccgggaa caccgtggcc acaccgacct cgacgatcaa
     1981 aaggccgctg gcccgctgat ccggtgggtt attctcgtcg tcacacttga tgtagaacgc
     2041 ctcctccggc actgctccgg cgagcgcgcc ctggctccac acgttggtca ggaacgagcc
     2101 gacgttgcgg atgatccgcg cccacaaatc ggggccattg ggctcgaaca ccgcccactg
     2161 ggtgccgtga tcgatcgact tgtagaggaa gctcaacaga cggcggatcg gcacgtactt
     2221 gtgcagcgac gtgtcaccgg cgagcgtccg ggcaccccac accttgatcg ccccgttcag
     2281 catccggatg cagttgccgc cttgcgggtt gatcgtgccc tgccccgtct tgccgatctg
     2341 gaccgacggg cggagcacgc cgaacagctg ctcgttggcg ggggccttgt gcacgccgcg
     2401 cgtggcgtcg acccgcgaat agatgccggc aatgtggccg ctcggcggca cggtcttctc
     2461 gcccttggtc gcgtcgtcga acacgaccag ccagggcgta tacagcgcgg cgaagtcggt
     2521 ggcctgatag gcggcggagc cgccggtcgg cagcagcggc gcgagctgcg tcggactggt
     2581 cgcgttcgcc ggcgcatcga ggatcgcgat ccgatcgccc atgttcctgc agtgcgtgac
     2641 gatatcggtg accgcggtca tgctggccgt cgccgccggc acccccggcg ccaccaccat
     2701 cgagatgtcg tcgaacgcct cgagcttcgg aagcacgacg gcgccgatgt cggtctcgac
     2761 cttggcgcgc acggcgtaac agaacgtccc gccattctcg aaaaagccct tcaccgcgtg
     2821 gccgagcgtg ttcagtccgg cattgagtgt ctgcccggta cccggaggcg ccgtgaagtc
     2881 gccgaacgcg gtcttgaact tggtgatgtt gaagattggg atcaccgtgc ccgagggatt
     2941 cgggtcgctc gcgccatcgg cccatacgcc gatgaacacg ggggtactgg tgccgacacc
     3001 aacgatcggt gcggtggccg ggacttcttc gacgaaaacg cctggtgcag tgtaagttgg
     3061 cataaggtga gttcctcttc gttccggacg cgcttccagt gcgcgcgagc gcagctagct
     3121 gtgactcaat gtgatgattg cagcggcggt ggtctgtccc tgtgacaggg tcaccgcctg
     3181 agtcgtggtg acgaagttcg tcgcgctgac ctcgagcgcg cggt
//
LOCUS       DASRJA010000318         9207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_119303,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000318 DASRJA010000000
VERSION     DASRJA010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119303"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /gene="bioB"
                     /locus_tag="VFC01_13760"
     CDS             complement(<1..792)
                     /gene="bioB"
                     /locus_tag="VFC01_13760"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HZN80733.1"
                     /translation="MSDILAEAREQVLERGDGLNQEQTLRVLQLPDDRLDELLALAHE
                     VRMAWCGPDVEVEGIISLKTGGCPEDCHFCSQSGLFASPVRSAWLDVPSLVEAAKQTA
                     KTGATEFCIVAAVRGPDERLLAQVAAGIEAIRNEVDIQIACSLGMLTQEQVDRLKDMG
                     VHRYNHNLETAQSYFPNVVTTHSWEERWGTLEMVREAGMEVCCGGILGMGETLEQRAE
                     FAANLAELDPHEVPLNFLNPRPGTPFGGLDVLPASEALKAVAAFRL"
     gene            842..1456
                     /locus_tag="VFC01_13765"
     CDS             842..1456
                     /locus_tag="VFC01_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HZN80734.1"
                     /translation="MQLHKRDVVDAATTLLDSYGIADLTMRRLARELDVSPGALYWHF
                     ANKQELLGAVADRVLDSVDDVPGDWRDRMTEICARLRDALLSHTDGAELVSASFAAGQ
                     SEAMARVLAALVNAATDAGVDPAHAELAARTVVYYVLGFTADEQSRMQWDAAGAELPD
                     DQSVLTEDPSARFAFGLRLLVDGIAAHSHAERELVLEERPQSRA"
     gene            1482..1808
                     /locus_tag="VFC01_13770"
                     /pseudo
     CDS             1482..1808
                     /locus_tag="VFC01_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351062.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
     gene            1818..2291
                     /locus_tag="VFC01_13775"
     CDS             1818..2291
                     /locus_tag="VFC01_13775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HZN80735.1"
                     /translation="MDLAGYAERVRNGPCFICGIVGGDESHEIFYEDDAHIAFLSNYP
                     TVPGYSLVSPKSHVEHAVASFDLDEYLAIQAVIHRVAGAVESVVPNERTYVLSLGSNQ
                     GNAHVHWHVVPCPRGLPYEQQQFHALMAEHGVLPWTRAQGYALGNKLRAALRGLN"
     gene            complement(2284..2793)
                     /locus_tag="VFC01_13780"
     CDS             complement(2284..2793)
                     /locus_tag="VFC01_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HZN80736.1"
                     /translation="MVHSVELVFDSDTEEAIRHIWDGLREAGIPSQAPASRPHVTLTV
                     AERIDPEVDELLTALGDRFPLPCVVGAPLLFGRSQLILTRLIVPTADLLALHAEVYRL
                     TLPHAHPDPMANSLPGQWTGHTTLARRVHGSGLGRALRVAGRPSEIKGSFVGLRRWDG
                     TAKQEYAIS"
     gene            complement(2793..3458)
                     /gene="bioD"
                     /locus_tag="VFC01_13785"
     CDS             complement(2793..3458)
                     /gene="bioD"
                     /locus_tag="VFC01_13785"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HZN80737.1"
                     /translation="MSVLVVTGTDTGVGKTVATAALACAARLAGIDVAVCKPVQTGSP
                     RDDDLADVARLSGVNALYGTWRYPEPLAPAAAAERAGMALPTRDELLGSVASVDAKLT
                     LVEGAGGLLVELGADGVTLRDLARELAAPVLVVVSPGLGTLNHTALTLEALAAQDIPC
                     AGLVIGAWPREPGVAEEGNRHELERLAAVRGVLPAGAGAMSVGEFESMSLAAFDPEWI
                     TSL"
     gene            complement(3455..4600)
                     /locus_tag="VFC01_13790"
     CDS             complement(3455..4600)
                     /locus_tag="VFC01_13790"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HZN80738.1"
                     /translation="MTRVGLSPLAWLDEVEQQRRADGLRRSLRTRPPVGAELDLASND
                     YLGLSQHPAVIDGGVDALRTWGAGSTGSRLVTGNTELHEGFETELADFVGAESALVFS
                     SGYTANLGAVVSLSGAGSLLVSDALTHASLVDACRLSRARVVVTPHRDVSAVEAALAA
                     RDEERAVVVTDSVFSADGVLAPLRSLHDVCRRHGALLIVDEAHGLGVRGSGGRGLLHE
                     VGLAGAPDVVMTTTLSKALGSQGGVVLGPAAVRAHLIDAARPFIFDTGLAPAAVGAAW
                     AALGVLIDEPWRAKAVLDHAAALAEMCGVSERPDSAVVSVILGEPEVAFATAVACLDR
                     GVRVGCFRPPTVPAGTSRLRLTARASLSDDEMALAREVLTEVLADRS"
     gene            complement(4632..5927)
                     /locus_tag="VFC01_13795"
     CDS             complement(4632..5927)
                     /locus_tag="VFC01_13795"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HZN80739.1"
                     /translation="MKVVLTPAEISAIDAAHVWHPYSTIGAEALPPVVAVGAKGAWLT
                     LIDDGRPVEVLDAMASWWTAIHGHGHPVLDAAITGQLATMNHVMFGGLTHEPAARLAE
                     LLVQITPDGLDTVFFSDSGSVSVEVAVKMALQYWRSIGRRDKHRLMTWRGGYHGDTFT
                     PMSVCDPDGGMHSLWTDVLAHQEFAPQVPTEYDPAYSEAFEKQLSSHADELAAVIVEP
                     VVQGAGGMRFHDPRYLVDLRAICDRHGVLLILDEIATGFGRTGELFAADHTGVGPDIM
                     CVGKAMTGGYITLAATLCTRDIAHTISTHEPGALMHGPTFMANALACAVGVASVQLLL
                     EGDWRATVSAIESGLRDGLAPAAALPGVADVRVLGAIGVIEMEKPVDLRVATPAALER
                     GVWLRPFRNLIYVMPPYICTSAEIEQITSAMVGVAGALT"
     gene            6003..8174
                     /locus_tag="VFC01_13800"
     CDS             6003..8174
                     /locus_tag="VFC01_13800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HZN80740.1"
                     /translation="MMTLAPPRPAPVTEPDSTPRANMGTRNSGFYRHDLDGLRGIAIA
                     LVAVFHVWFGRVSGGVDVFLALSGFFFGGRLLRAALTPGTSLSPVPEVARLVRRLLPA
                     LVVVLAASAMLTILIQPETRWETFADQSLASLGYFQNWELANTASDYLRAGEAVSPLQ
                     HIWSMSVQGQFYIAFLVLIFSFAYLLRRRLGKYMRISFVVLLSSLTIASFAYAIFAHN
                     ADQATAYYNSFARAWELLLGALLGALVPYVRWPMWLRTTVAVAALAAILSCGALIDGV
                     KEFPGPWALVPVGATMLFILSAANRQADPRTAGRLPAPNRLLATAPFMTLGAMAYSLY
                     LWHWPLLIFWLSYTGQPRVNFIEGGIVLLLSGVLAWLTLNFVENPLRYRASTKTPKPI
                     VPLWTRLRRPTIVLGSIVGLLGVALTATAFGWREHVTVQRANGKELANLSIRDYPGAT
                     ALINYAKVPKLPMRPTVLEAKDDLPETTTDGCISDFDNSDVINCTFGDKQATRTIALA
                     GGSHAEHWITALNLLGQLHHFKVVTYMKMGCPLTTEEQPLVMGDNRPYPGCRVWNNRV
                     MPQLISDHPDFVFTTSTRPWNIKPGDVMPATYIGIWQTLSDNNIPILAMRDTPWMVRK
                     GQPYFPADCLANGGDAISCGIKRSDVLSEHNQTLDFVAQFPLLKPLDMSDAVCRKDIC
                     RAVEGNVLLYRDAHHISTTYMRTMAGELGRQIGAATGWWVG"
     gene            8174..>9207
                     /locus_tag="VFC01_13805"
     CDS             8174..>9207
                     /locus_tag="VFC01_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZN80741.1"
                     /translation="MSSASSVSTVWPGESYPLGATYDGAGTNFSLFSEVAERVELCLI
                     AKDGTEERINLDEVDGYVWHCYLPTVTPGQRYGFRVHGPWDPAAGHRCDPSKLLLDPY
                     GKSFHGDFEFSQALFSYDLGADDLASGGTPPMIDSLGHTMTSVVINPFFQWASDRAPR
                     TPYHETIIYEAHVKGMTQTHPGIPEELRGTYAGLGHPVIIDHLKSLNVTAIELMPVHQ
                     FMHDHRLLDLGLRNYWGYNTFGFFAPHYQYAATRHAGGAVAEFKTMVRSFHEAGIEVI
                     LDVVYNHTAEGNHLGPTINFRGIDNAAYYRLQDGDLRVYKDFTGTGNSLNPRHPHTLQ
                     LIMDSLRYWVL"
BASE COUNT         1569 a         3210 c         2889 g         1539 t
ORIGIN      
        1 cagccggaac gcggcgacag ccttcagcgc ctcggatgcg ggcagcacat caaggccgcc
       61 gaaaggtgtg cccggtcgcg ggttgaggaa gttcagcgga acctcgtgag gatccagttc
      121 ggcgaggttg gccgcgaact cggcgcgctg ttcgagcgtc tcccccatcc cgaggatgcc
      181 gccgcagcac acctccatgc cggcctcgcg gaccatctcc aacgtccccc accgctcttc
      241 ccacgagtgg gtggtgacga cattggggaa gtaggactgc gcggtctcca ggttgtggtt
      301 gtagcggtgc acgcccatgt ccttgaggcg atccacctgc tcctgggtca gcatgcccag
      361 cgagcacgcg atctggatgt cgacctcgtt gcggatcgcc tcgatgcccg cggccacctg
      421 cgccagcagg cgttcatcgg gtccgcgcac ggcggcgacg atgcagaact ccgtcgcgcc
      481 ggtttttgcg gtctgcttgg cggcctcgac gaggctcgga acgtccagcc aggcgctgcg
      541 caccggcgac gcgaaaagcc ccgattgcga acagaagtgg cagtcctcgg ggcagccgcc
      601 ggtcttcagg ctgatgatgc cctcgacctc gacgtcgggc ccgcaccacg ccatccgcac
      661 ctcgtgcgcc agggccagca gctcgtcgag ccggtcgtcg ggaagctgca gcacccgcag
      721 cgtctgctcc tggttcaggc cgtcgccccg ctccagtacc tgctcgcgag cctcagccag
      781 aatgtcgctc acccgtcgcc ctccatcgct tcttgaacgg tgttcaggtt agggtaccgg
      841 tgtgcagctc cacaaacggg acgtggtcga cgcggcgaca acgctgctgg actcctacgg
      901 catcgccgac ctgacgatgc ggcggctggc ccgcgagctc gacgtctccc ccggcgcgct
      961 gtactggcat ttcgccaaca agcaggagct actcggtgcc gtcgccgacc gggtcctgga
     1021 ttccgtcgac gatgtgcccg gcgactggcg tgaccgcatg acagaaatct gtgcgcggct
     1081 gcgcgacgcg ctgctgtcgc acaccgacgg cgccgagctg gtgtcggcga gcttcgcggc
     1141 cggacagtcc gaggcgatgg cgcgggtcct ggcggcgctc gtcaacgccg cgacggacgc
     1201 cggcgtggat ccagcgcacg ccgagctggc cgcccgcaca gtcgtgtact acgtgctggg
     1261 cttcaccgcc gacgagcaat cacggatgca atgggacgcg gcaggcgccg agctgcccga
     1321 cgaccagtcg gtgctgacgg aggatccgag cgcgcggttc gcattcgggc ttcgattgct
     1381 tgtcgacggg atcgcagcgc atagtcacgc cgaacgtgag cttgtgctcg aagaacggcc
     1441 gcaatctcgc gcataagctc acgttcgagg aggttcggaa cgtgatcacg gtcgacgatg
     1501 tgcggcccat cgccttgtcg ctgccgaggg cctacgaggt ggtggtgcgc gaccagatca
     1561 agttccgggt cgggcagatc gtcttcgttg cgttctcgcg ggacgagact ttgatgggcg
     1621 tcggctttcc caaggaggaa cgcgcggcga tggtggccgc cgaacccgag aagttcatgc
     1681 tgcccagcga atcggacatg agatacaact ggattcacgt tcggctcgac gcgatcgacc
     1741 tcgaggaact gcgcgagctt gtcatcgacg cctggctgat gtgcgtgccg aaaaaggttg
     1801 cagcatcgcg aattccgatg gatctcgccg gctacgccga gcgagttcgc aacgggccct
     1861 gcttcatctg cggcatcgtc ggcggcgacg agtcgcatga gatcttctac gaagacgacg
     1921 cgcacatcgc cttcctgagc aactatccaa ctgtgccggg ctattctctc gtttcaccga
     1981 aatcgcacgt cgagcacgcc gtcgccagct ttgatctgga cgaatacctt gcaattcaag
     2041 cggttatcca tcgcgttgct ggcgctgtcg agtccgtcgt gccaaatgag cgcacctacg
     2101 tcctctcgct cggcagcaat cagggcaatg cgcatgtcca ttggcacgtc gtgccttgcc
     2161 ctcgcggtct gccgtacgag cagcagcagt tccacgcact gatggccgag cacggtgtgc
     2221 tgccctggac gagggcgcag ggctatgcgc tcggaaacaa gcttcgcgcc gccctgcgag
     2281 gtctcaactg atcgcgtatt cctgcttggc ggttccgtcc cagcggcgga gcccgacgaa
     2341 gctgcccttg atctcggacg gccgtcccgc cacacgtaat gcccggccga gtccgctccc
     2401 gtgcacacga cgggcgagcg tcgtgtgacc tgtccactgc ccgggcagag agttggccat
     2461 cgggtcagga tgcgcgtgcg gcagggttaa ccggtacacc tcagcgtgca gagccagcag
     2521 atcggctgtc ggcacgatca agcgggtcag gatcagctgc gaccgaccga atagaagggg
     2581 cgcgccgacc acacacggca acgggaatcg gtcgcccaag gccgtcagca actcatccac
     2641 ctcggggtcg atccgctcgg ccaccgtcag cgtcacatgt ggtcgactcg ccggcgcctg
     2701 actcggaatc ccagcctcgc gcaatccatc ccagatgtgc cggatcgcct cctcggtgtc
     2761 cgagtcgaag accagctcga cggagtggac catcagaggc tcgtgatcca ctccgggtcg
     2821 aacgccgcca agctcatcga ctcgaactcc ccgacactca tcgcccccgc acccgcgggc
     2881 agcacaccgc gcaccgccgc caaccgttcc agctcatgcc gattgccttc ttcggccaca
     2941 cccggttcgc gtggccaagc gccgatcact agcccggcgc atggaatatc ttgagcggca
     3001 agcgcctcca gcgtcagcgc ggtgtggttc agcgtgccga gtcccggtga cacgacgacc
     3061 agcaccggcg cggccagctc gcgcgcgagg tcgcgcagtg tcaccccgtc ggcgccgagc
     3121 tcaaccaaaa ggccacccgc accctcgacc agcgtcagct tcgcatcgac cgacgcgacc
     3181 gaacccaaca actcgtcgcg cgtcggcaac gccatgcctg cacgctctgc ggccgccgcc
     3241 ggcgccagcg gttccggata gcgccacgtg ccgtacagcg cgttcactcc ggacagtcgc
     3301 gccacatctg cgaggtcgtc gtcgcggggg ctgcctgtct gcacgggttt gcacaccgcg
     3361 acgtcgatgc ctgcgaggcg tgcagcgcag gccagcgccg cggtcgccac cgtctttccg
     3421 acgccggtgt cggtgcccgt gacgaccaga acactcacga gcggtcggcg agcacttcgg
     3481 tgagaacctc tcgggccaag gccatttcgt cgtccgacaa cgacgctcgc gccgtcagcc
     3541 gcaaccgcga cgtaccggcg ggaacagtcg gcggacggaa acagccgacc cgcacaccac
     3601 gatccaggca ggccaccgcg gtagcaaaag ccacctccgg ctcgccgaga atcaccgaca
     3661 ccaccgccga atccggacgc tcggacacgc cgcacatctc ggcaagcgcg gcagcgtgat
     3721 ccagcacggc cttcgcgcgc cacggctcgt cgatcagcac gcctaaggca gcccacgcgg
     3781 caccgacagc agcgggcgcc agcccggtat cgaagatgaa cggtcgtgcc gcatcgatca
     3841 gatgcgcgcg cacagctgcg ggtccgagca ccacaccgcc ctggctgccc agcgccttcg
     3901 acagcgtcgt cgtcatcacc acgtcgggcg caccggccag gccgacctca tgcagcaagc
     3961 cgcgcccacc gcttccccgg acgccgaggc cgtgtgcctc gtcaacgatc agcagtgcgc
     4021 catgccgccg gcacacgtca tgcaaagacc gcagaggcgc gagcacccca tcggccgaga
     4081 acaccgaatc ggtcaccacg acggcgcgct cctcgtcgcg cgcggccagc gccgcctcga
     4141 cggccgagac gtcacggtgg ggtgtcacca ccacgcgagc gcgcgacagc cggcaggcgt
     4201 ccaccagcga cgcatgcgtc agcgcatccg acaccaacag ggatccggct ccggacagcg
     4261 acaccaccgc gccgaggttg gcggtgtaac ccgacgagaa caccagcgcc gactctgccc
     4321 cgacgaaatc cgcgagctcg gtctcgaaac cttcatgcag ttcggtgttg ccggtgacca
     4381 gccgcgatcc cgtcgagccc gcgccccagg tgcgcagcgc gtcgacgccg ccgtcgatca
     4441 ccgctgggtg ctgcgacagg ccgaggtagt cgttggaggc caggtcgagc tcggcgccga
     4501 cgggcgggcg ggtgcgcagg gatcggcgca gtccgtccgc gcgccgctgc tgctcgacct
     4561 cgtcgagcca ggccagcggg gagagaccga cgcgcgtcac ctgaccacct tccctgacac
     4621 ttgaacggtg ttcaggttaa cgcaccggcg acaccgacca tcgccgaggt gatctgctcg
     4681 atctccgcac tcgtgcagat gtacggcggc atgacgtaga tcaggttgcg gaacgggcgc
     4741 agccagacac cacgctccag cgccgccggc gtcgcgaccc gcagatcgac gggcttctcc
     4801 atctcgatca cgccgatcgc gccgagcacc cgcacatcgg ccacccccgg cagcgcggcc
     4861 gcgggcgcta acccgtcgcg caggccagac tcgatcgcgg aaaccgttgc tcgccaatcc
     4921 ccttcgagta gcagctgcac cgacgccacc cccaccgcgc atgccagcgc gttggccatg
     4981 aacgtcgggc cgtgcatcag cgcgccgggc tcgtgggtgc tgatcgtgtg cgcgatgtcg
     5041 cgggtgcaca acgtcgcggc cagcgtgatg tagccgccag tcatcgcctt gcccacgcac
     5101 atgatgtccg ggccgacgcc cgtgtggtcg gcggcgaaca gctccccggt gcggccgaag
     5161 ccggtggcga tctcgtcgag gatcagcagc acgccgtggc ggtcgcagat cgcacgcaag
     5221 tcaacgagat accgcggatc gtgaaagcgc atgccgccgg cgccctgcac gacgggttcg
     5281 acaatcacgg cggccaactc gtccgcgtgc gaggacagct gcttttcgaa ggcctcgctg
     5341 tacgcggggt cgtactcggt cggcacctgc ggcgcgaact cctgatgagc aagcacgtcg
     5401 gtccacagcg agtgcatgcc gccgtccggg tcgcacacgc tcatcggcgt gaaggtgtcg
     5461 ccgtggtagc cgccgcgcca ggtcatcaac cggtgcttgt cgcggcggcc gatgctgcgc
     5521 cagtactgca acgccatctt gaccgcgacc tccacggaca ccgagccgga gtcgctgaag
     5581 aacaccgtgt cgagtccgtc gggcgtgatc tgcaccagca gttcggccag ccgggcggct
     5641 ggttcgtggg tcagcccacc gaacatgacg tggttcatcg tcgcgagctg gccggtgatc
     5701 gccgcatcga gtaccggatg gccgtggccg tggatcgccg tccaccacga cgccatcgcg
     5761 tcgagcactt cgaccggacg gccgtcgtcg atcaacgtca gccaggctcc cttcgcgccg
     5821 accgccacca ccggaggcag cgcctcggcc ccaatggtgc tgtaggggtg ccagacatga
     5881 gcggcgtcga tcgcactgat ctccgctggg gtcaacacga ccttgatcct ttgctcaccg
     5941 ggttcgacgg atcgcccgcg gcatgagcca ccaggcgatc ttcggtagat taaccgtcga
     6001 ccatgatgac cctcgcccct ccccggccgg cgcccgtcac cgagccggat tccaccccgc
     6061 gcgccaacat gggaacccgg aactccggct tctaccggca cgacctcgat ggcctgcgcg
     6121 gcatcgccat cgccctcgtt gcggtcttcc acgtgtggtt tggccgggtt tccggtggtg
     6181 tcgacgtttt cttggcgttg tcgggtttct tcttcggcgg gcgtctgctg cgcgcggcgc
     6241 tgacgccggg aacgtcgctg tcacccgttc ccgaagtcgc ccgacttgtt cgccggctgc
     6301 tgccggcgct cgttgtggtg ctggccgcgt cggcgatgct gacgattctg atccagcccg
     6361 agacgcgttg ggagacgttt gctgaccaga gcttggcgag cctcggctac ttccagaatt
     6421 gggagctcgc gaacaccgca tcggattacc ttcgcgccgg tgaggcggtc agtccgctgc
     6481 agcacatctg gtcgatgtcg gtccaaggcc agttctatat cgccttcttg gtcctgatct
     6541 tttcgttcgc ctatctgttg cgacggcgtc tggggaagta catgcggatt tcattcgtcg
     6601 tgctgctgag ctcattgacc atcgcgtcgt tcgcctacgc gatatttgct cacaacgccg
     6661 atcaggcgac cgcctactac aacagtttcg cccgggcgtg ggagctgctg ctgggcgcct
     6721 tgctcggcgc cttggtgccg tacgtgcgct ggccgatgtg gctgcgcacg accgtcgcgg
     6781 tggccgcgct ggcagcgatc ctgtcgtgcg gcgcgctgat cgacggcgtc aaggagttcc
     6841 ccgggccgtg ggcgctggta cccgtcggcg ccaccatgtt gttcattctt tcggccgcca
     6901 atcggcaggc cgacccgagg accgcagggc ggctgcccgc ccccaaccga ttgctggcca
     6961 ccgcgccatt catgacgctc ggggcgatgg cctactccct gtacctctgg cactggccgc
     7021 tgttgatctt ctggctgtcc tataccgggc agccgcgcgt caacttcatc gagggcggga
     7081 tcgtcctgct gctctcgggt gtgctggcct ggctgacgct caatttcgtc gagaacccgc
     7141 tgcggtatcg cgcctcgacg aagacgccga aacccatcgt tccgttgtgg acgcggctgc
     7201 gtcgcccgac gatcgtgctg ggctccatcg tcggactgct gggtgtcgcg ttgacggcga
     7261 cggctttcgg gtggcgcgag cacgtgacgg tgcagcgcgc gaacggcaaa gagttggcga
     7321 acctttcgat ccgcgactat cccggcgcca cggcactgat caactacgcc aaagtgccca
     7381 aattgccgat gcgcccaacg gtgttggaag ccaaagacga tctgccggag accaccaccg
     7441 acggctgcat cagcgatttc gacaacagcg acgtgatcaa ctgcaccttc ggcgataaac
     7501 aggcgacccg caccatcgcg ctcgccggtg gatcgcacgc cgagcattgg atcaccgcgc
     7561 tcaacctgct cgggcagctg caccacttca aagtggtgac gtacatgaag atgggttgcc
     7621 cgctgaccac cgaggaacag ccgctggtga tgggcgataa ccgcccatac ccgggatgcc
     7681 gtgtgtggaa caaccgcgtc atgccccaac tcatctccga ccatccggat ttcgtgttca
     7741 ccacatccac ccggccgtgg aacatcaagc ccggcgatgt gatgccggcg acatacatcg
     7801 ggatctggca gacgttgtcg gacaacaaca ttccgatcct ggccatgcgc gacactccct
     7861 ggatggttcg taagggacag ccctacttcc ctgccgactg cctggccaac ggcggcgatg
     7921 cgatctcctg cgggatcaag cgatccgacg tactgtcgga gcacaaccag acactggatt
     7981 tcgtcgcgca gtttccactg ctgaagccgc ttgacatgag tgatgcggtg tgccgcaagg
     8041 acatctgccg cgctgtggag gggaatgtgt tgctgtatcg cgacgcccac cacatctcga
     8101 cgacgtacat gcggacgatg gcgggagaac tcgggcgcca gatcggcgca gccaccggct
     8161 ggtgggttgg ctgatgtctt ccgcgtcgtc ggtctccacg gtctggccag gagagagtta
     8221 tcccctcggc gccacctatg acggcgccgg taccaacttc tcgctgttct ccgaggtggc
     8281 cgagcgtgtc gagttgtgcc tgatcgccaa ggacggcacg gaggagcgga tcaacctcga
     8341 cgaggtcgac ggctacgtat ggcactgcta cctaccgacc gtcactcccg gtcagcgcta
     8401 cggcttccgc gtgcacgggc cgtgggatcc cgcggcgggc caccgctgcg acccaagcaa
     8461 gctgctgctc gatccgtacg gcaagtcgtt tcacggtgac ttcgaatttt cccaggcgct
     8521 gttctcctac gacctgggag ccgacgatct cgcctctggc ggcacgccac cgatgatcga
     8581 ctcgctcggc cacaccatga ccagcgtggt gatcaacccg ttcttccagt gggcgtcgga
     8641 ccgtgcgccg cgcacgccgt accacgagac catcatctac gaggcgcacg tcaaagggat
     8701 gacgcagacc catccgggca tccccgagga actgcgcggc acctacgccg ggctcgggca
     8761 cccggtgatc atcgaccacc tcaagtcact caacgtgacc gcgatcgaac tgatgccagt
     8821 gcaccagttc atgcatgacc atcggctgct cgacctgggc ctgcgaaact actggggcta
     8881 caacactttc ggtttcttcg caccgcacta ccagtacgcc gccacccggc acgccggcgg
     8941 agccgtggcc gagttcaaga ccatggtcag gtcgttccat gaagccggaa tcgaagtcat
     9001 cctcgacgtc gtctacaacc acaccgccga gggcaaccac cttggtccca cgatcaactt
     9061 ccgcggcatc gacaacgccg cctactaccg cctgcaggac ggcgacctga gggtctacaa
     9121 ggatttcacc ggcacgggca acagcctcaa cccgcggcac ccgcacaccc tgcagctgat
     9181 catggactcg ctgcggtact gggtgct
//
LOCUS       DASRJA010000319        21003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3983322,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000319 DASRJA010000000
VERSION     DASRJA010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 21003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21003
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3983322"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1310
                     /locus_tag="VFC01_13810"
     CDS             111..1310
                     /locus_tag="VFC01_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80742.1"
                     /translation="MYLDFTPEQKQLRTEIRSSLETVMTPERSAAVAGRMEGGDAVKE
                     CVRALGAAHLLGVGWPKEYGGRGFTALEQFIFFEEAQRVNAPIPLVTLNTVGPTLADC
                     GTDEQKQKFLPAILNGTVEFAIGYSEPDAGSDLASLRTTAVRDGDGYVINGQKMFTSG
                     AAYADYIWLAARTDPTVKKHKGISIFIVPTSSPGFSWKPLHTMPGVSTFYTFYDDVRV
                     PASSIVAGVNEGWRLITTQLNFERAALGNMGALEPLFEKTLDWARTTELDGGRVIDKP
                     WVQQAMARVEAQVAAYKIMNLRVNAAMTKGALGMGEASAVKVFGTELTQRVARELLEV
                     LDGAGTRKGEDAPLRGALESAYRIAVINTFGGGANELQRDIIAMAGLFMPRAPRDLRA
                     SQIGNNK"
     gene            1409..1915
                     /locus_tag="VFC01_13815"
     CDS             1409..1915
                     /locus_tag="VFC01_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HZN80743.1"
                     /translation="MGQPTGGSGKPTVAPDPVNQPMIRHWAHALSDMNPIYLDPEFAA
                     KSRFGGIVSPPVMLQAWTMPAPKIEGIAERGGVPMEMGKNPAAFIEEAGFTGIVATNS
                     EFEIERYPRLGDIISVTQVFEDISDEKKTSLGTGHFITWLATYTDQNGEVLGRQRFRV
                     FRFKAGAS"
     gene            1915..2325
                     /locus_tag="VFC01_13820"
     CDS             1915..2325
                     /locus_tag="VFC01_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HZN80744.1"
                     /translation="MATRLAPTVSPDAEFFWNGLRDHKLLIQRCTGCQALRQPPRPMC
                     PTCNSLDWDTVDATGRGTVYSYVMPQHPPMPLMEYPYIVALVELDEGVRLVSNLRDIA
                     PADVEVGMPVEVFYETFDTIDGKDIVLHQFRPAR"
     gene            2335..3438
                     /locus_tag="VFC01_13825"
     CDS             2335..3438
                     /locus_tag="VFC01_13825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80745.1"
                     /translation="MDFTFTEEQETIAKVARQLFEHRATPEHLTELEAGDVRYDADLW
                     RELATADLLGIALPENVGGSGHGFLELALLLGEVGWSVAPVPVYATLLLGADAIARHG
                     DEGQRQRYLPDVVNGARLLTAGLAEPGRSDPTAPATTARRDGGGWRIEGSKDLVPAAQ
                     VAHTMLIPATDDDGDVGLFLIDVDAGGVEIRPATTTNGEPHADVFLDGAVVSGQDRLN
                     ANGAEMIASLYNRALVGLCAIQLGVTERALRIAAAYTTEREQFGRPIGSFQAVQQRMA
                     DAFIDVEAIRWTMWQAAWLLGEGKPASREASIAKFWAGEAGARVAATAQQVHGGIGID
                     TTYPLFRYFLWAKHNELTLGSASAQLARLGAAY"
     gene            3451..3903
                     /locus_tag="VFC01_13830"
     CDS             3451..3903
                     /locus_tag="VFC01_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZN80746.1"
                     /translation="MTTDKLRTTTLGWRDINVGDEVTPLEIPVTATVIVAGAIASRDF
                     MPVHHDPEFAKKQGSPNIFMNILTTNGLCVRFLTDWAGPEAMVTKLAIRLGVPAFPND
                     PLTFTGSVTGQSEGVDGEGFVEVTFKGSNSLGDHVSGTATLSLLAEAS"
     gene            3900..5066
                     /locus_tag="VFC01_13835"
     CDS             3900..5066
                     /locus_tag="VFC01_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HZN80747.1"
                     /translation="MSLGGKAAIAGIGQTEFSKESGRSELQLACEAVNAAIDDAGLRP
                     SDVDGMVTFTMDSSDEIEIARNVGIGDLTFFTRVHHGGGAAAGTVLQAAMAVATGTAD
                     AVVCYRAFNERSGFRFGGATARPTAETPLFMANYAPFGLLTPAAWVALHAQRYMSTYG
                     ITNEDFGVISVVDRAHAARNPDAWFYERPITLQDHQNSRWVIEPVLRLLDCCQESDGG
                     VALVITSAERARDLRHPPAVITAAVQGAAAEGEMMTSYYRDDITGLPEMGVVAEKLWR
                     DSGLKPGDIQTAFLYDHFTPFVFAQLEELGFCGRGEAKDFATVERLSLGGELPINTNG
                     GLLGEAYIHGMNGITEGVRQVRGTSCNQVEDVEHVLVTSGTGVPTSALILAPAV"
     gene            5074..5676
                     /locus_tag="VFC01_13840"
     CDS             5074..5676
                     /locus_tag="VFC01_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80748.1"
                     /translation="MTTETTAADTRELIIESAFGCFRRQGLDKTTIVDIARAADISRS
                     TVYEYFRDKSEIVEACAEHSSQRFYREMAKAMGPGESLEEKLTQAAVFVARARRFIEP
                     EMYFDADEVSLLLTKNAAVLLRECCEFLAPYLTAAKLTGEVRKDLDVMAAGEWFARML
                     FSLFSTPSSTLNMDDDAIVADFVRSHVVRGFANDRAGRSR"
     gene            complement(5757..7109)
                     /locus_tag="VFC01_13845"
     CDS             complement(5757..7109)
                     /locus_tag="VFC01_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80749.1"
                     /translation="MTNILDLADQALFLGERATGATSVIQCIWVYDRAIDVDGLRRFH
                     HHLQRGRLARRIERSPLPFGRHRWVTSSDSSDIEFTQTPRPREEFDDWLTNQAHTPLD
                     AEHGPGWHLAVVPFTDGGTGVSLVVAHALIDGVGLAMALVEATSGVDDAIMWPPARSR
                     RRWRALGQDARQTVRDIPAVGRAARAAIRMARRHRRDAGAPARASKHVDSRDERITLP
                     GATAFIDASEWDARAQSLGGTSNALLAGLAADAAQRMGRVTVGGDAVMLSIPVNERTD
                     GDTRANAVTNVDVTVDPTDATTDLRGIRAAIKQALIYHNNVPDERLALLPIVPLVPKR
                     LMRRMVSVATGNAASVVSSNLGEVPSAVTRPDGTDAEYCTARSVYPGMTRATMHRTGG
                     VLGFISGRVNGKVGISVLSYQPGRPNSDVELRQTLSSTFAAFSLNATMQWESTELVAH
                     "
     gene            complement(7142..8596)
                     /locus_tag="VFC01_13850"
     CDS             complement(7142..8596)
                     /locus_tag="VFC01_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HZN80750.1"
                     /translation="MVSLGPIHSWHPAAGTVTTWTASSAARESASRASRSDLPPSFQQ
                     AQHLRGAFYGKALGRELPRLMVVAWDIPGECDIALMTEAINTHVRRHDTYHSTFEFEN
                     GNIFRRTIDDPKEIEFVPSSFGSMEQEQIRAHALAMTSETLEWDCFSFGVIQSADHFT
                     VYASVDHLHIDGMSAGLIFVDLHLMYQNLSQGQPVTLPEVGGYRDYTARQREKVASMT
                     LSSPEIKDWIAFAQSTDGDWPSFPLELGDTWANNKGDFVTVELLNEDDTEAFDTACRE
                     AGARFSGGVLACAALAEHQLTGNTTYHGFTPSDTRTADIDTMSVGWFASLFPVAVPIG
                     DGEFAEIARAAQKSFNAGKCLASVPFERVLELAPVDQLGIKLPTRPGMMVSFLDFRKI
                     PVAELWEQTNYGIYGDNLSHGGINMWINRHAGRTTVTISFPDNPIARKSVHRYIAALS
                     KAFADVAKITADWIEELAHHANSSDSCAVCAGSR"
     gene            8892..11864
                     /locus_tag="VFC01_13855"
     CDS             8892..11864
                     /locus_tag="VFC01_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND family transporter"
                     /protein_id="HZN80751.1"
                     /translation="MLKKATTKTPKNFDGFSLLGRAVVRAPWLVISAWITLVVVLFMA
                     FPALTKVVENQTIQPLPPQAMAATDQMAKDFHESAQNILVVVMTNEHGLNPADNNTYR
                     AVADKLRSDTSDVSAVQDFISTPPLRQLMVSKDNKAFYMAVTLKAPPGSPESSQAYQQ
                     ITQMVKQATAGSDLTTHVTGSAAVIGDLSIVTAHDMHVIEIATAALVLLILLVIYRRP
                     VTVLLPLITIGISVASAQGLVSALTEIGMSVSALTIVLMTAMIVGAGTDYAVFLISRY
                     HEYIRSGMDSDAAVEKALTSIGKVIGASAATVAVTFLGMIFTQLPAFTSVGPALAVSI
                     AVAFLAAVTLLPAVLVLAGRRGWIAPRRPLTDRLWQRSAVHIVRKPKSHLFVSLTILI
                     ALGACALFMNPTYNDRMQLPGSAESNLGYSAMADHFSTSALLPEYIYIHSPHDLRNPQ
                     SLADMEQMAQRVAQLPNVAAVRGVTRPTGQPIEQTKVSYQAGEVGSKLEDASSQISDK
                     SSDLDKLSGGAHQLAGSLAQVRDQVNGAGRSMTAMTATLSQVQNQLDGPQTTQMLNTI
                     RTYANTVGGNQEAMNGVANSAPAMLNALSNNPQCDADPVCSDGRAKLQQLASTGYSQT
                     GASAQSILSSVQRLSSMMQSAGTSLRSAGINSPQGAQAKIAQMQQGANALANGSAQLA
                     QGVQVLVDQTKKMGGGLNQAASLLLSIKHDASSPSMAGMYVPPQVLTTNDFKNAAKVF
                     ISPDGHSARYMVETKFDPFSTDAMDQVQTILDTARGAQPNTTLSDATISMVGTTPMYS
                     AIRSYYDHDLRLIIVLTLVVVFLILVALLRAIVAPLYLIASVVISYLSALGLGVAFFQ
                     FICHQHIYWNVPATAFIVLVAVGADYNLLLISRIREESRNGIRSGIIRAVHSTGGVIT
                     SAGIIFAASMFGLMFGSVSTMLQIGFIIGAGLLIDTFVVRTITVPALAALIGRANWWP
                     TKSRKAAPGRKAVDAHAG"
     gene            12146..12760
                     /locus_tag="VFC01_13860"
     CDS             12146..12760
                     /locus_tag="VFC01_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZN80752.1"
                     /translation="MTRTNLSMSISADGYVAGPGQCEEQPLGVGGRTLHDWHVGPTKN
                     HPVNRQVVSDMMDGMGATIMGRNMFGPIRGEWEDSDWKGWWGDVPPYHCPVFVLTHYP
                     HDPIEMEGGTTFHFVTDGIESAYAQAQAAAGDHAISIAGGASCARQAIAAGLVDEIDL
                     QINPVILGSGERLFDGFEAGKPALELVRVLEAPGVAHLRYRVVR"
     gene            12957..13333
                     /locus_tag="VFC01_13865"
                     /pseudo
     CDS             12957..13333
                     /locus_tag="VFC01_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
     gene            complement(13440..14258)
                     /locus_tag="VFC01_13870"
     CDS             complement(13440..14258)
                     /locus_tag="VFC01_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN80753.1"
                     /translation="MTLHGDRVAYRDEGAGEVLLLVHGMGGSSNTWSGVIPLLAKKYR
                     VIAPDLLGHGESDKPRGDYSVGAFAVLLRDLLDALDVPRVTVIGHSLGGGVAMQFAHQ
                     HRQYCERIVLISSGGFGDDVGRVLRLLSLPGSELVLPVIASRPAVLAGNALRALMGSS
                     DRFKARPSLSNRDNRQAFLRTLRSVVDFRGQAVSALNRLGFHGVLPALIISGDQDRVI
                     PVEHARAAHRMLPNSRLHIMPGVQHHPPTERPETVARLIDDFVVTTAEDTPRAA"
     gene            complement(14632..15651)
                     /locus_tag="VFC01_13875"
     CDS             complement(14632..15651)
                     /locus_tag="VFC01_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN80754.1"
                     /translation="MSVAPDSGLAPAVLRPAALTGDGCVERTVTTKDGVRLAVSDYSS
                     DRADAPTVVLLHGLLLSQESWEPQVRQLRRRYGSRIRIVTYDHRGHGRSTGSPMPTYE
                     IAQLAADLAEVLTALHITGPLTLAGHSMGGMTALAYFGRPASKRPVEPRGLVLVGTAA
                     GRLAARGIGRLLATPATGALFELVHRMPQRATDKVVQALIRPLGEALGRFSSDGTAVA
                     AVAATAIRTTSLTTAAGFLPSLKRYDVYQALEAISAKTFVVSGGTDILTPASHSHDLA
                     AAIPGAVHLHHPTAGHMLLQEAAACVSDAIGRAMGMRRGSRRPATARKSRNRSSAAQL
                     AVVVS"
     gene            15973..16659
                     /locus_tag="VFC01_13880"
     CDS             15973..16659
                     /locus_tag="VFC01_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80755.1"
                     /translation="MTTWPSNHSHDQWSAATMAACIPQTVSMRAAAEFNATPTAARPY
                     MADLAWSAEPPGYASPLPIQRRRRWFTRSRVLTFVGGGLVAAAAAGLFGALLGAQSTP
                     VDVTRHSAPPAAPVAAPANPAPTPGMQTMPATTNHSVIASHRASSHSAGHHSPPPPQQ
                     YSTPPSSGQNTNIQQRPCDHDTVSTPPTWNRNFFYWLTHRRDHDGSRWTHDQSSDNHS
                     TGSGDNGHSN"
     gene            16664..18049
                     /locus_tag="VFC01_13885"
     CDS             16664..18049
                     /locus_tag="VFC01_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN80756.1"
                     /translation="MTATLPADQRDIGRRLDHLPVGPVHRRVVIAVGLGLFFEVYEIF
                     LSSTIATALSTQYGLGGTALQLLLASSFLGMFFGAAAFGRIADRIGRRRAFLLNLVWF
                     SVWSVIGAVAPSPWILVGARFMAGIGVGAEYPVADAYLSDVLPMAHRGRLAAWAYTCS
                     FLAVPVLGFLSLSLTGRSLFGVEGWRVLLLVGALGALFVIVLRRGLPESPRWLASVDR
                     IADARAALRAFESGTGPAPEPAADSEGDDDVAAASEPGRTVSALQRLRLSPYRQRLIM
                     LGVFHLFQPFGYYGFGTLAALVLVSRGYDVTASLLFTAVSFIGYPIGSLVSIPLLKRF
                     ERKNLIIVMVAVMATAGVLFATVNVPALIVVFGLLITAASNIFSNIYHVYQAEIFPSD
                     VRATAVGWTYSVSRLSSAALPFILIPVLDNYGATAMFAAVLVALAATIAVIAPIGPRT
                     TGKSLDEINPT"
     gene            18124..19812
                     /locus_tag="VFC01_13890"
     CDS             18124..19812
                     /locus_tag="VFC01_13890"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZN80757.1"
                     /translation="MVRRLHVDVRLATQVLLLQVAVVTLTLGIAGGLVAFMSHQRLAA
                     QYETRSLDMARAIAFAPAVRADVARYDNAPLTPSPALTDELANGQLQQLATEIQQRTG
                     VLFVVITNNQGIRLTHPNRDELGKHVSTDPSEALAGHEVVTRETGTLGRSVRAKVPVL
                     APDSNRVIGEVSIGISTAAVHQQLWTDVRTAAVLVGLALLIGVVGSLFLARRWRGLTM
                     GLQPSEMAELIRGQAAVLHGIDEGVLAVDTDWKTTFVNDEACRLLEVSNESGRPVDEI
                     GLTPRVLEVFRSADPTPTLATVGDRVVVVSARHVARDDRDLGTVLVVRDRTDVESLTR
                     QLDAVQLMSTVLRAQRHEFANRLHLINGLLHSGHTEEAAQYVEELLGSGPLGSALPGI
                     DTVRDAFLQAFLAAKAASAREAGVTLTIGENTWVPGRLALPVDVTTVLGNLVDNAIDA
                     ARTGANEAKVVEVELLQDGSTLHITVADTGDGVAPDFVDDVFTEGKSTKPESGIPGGR
                     GIGMALSRQISRALGGDVRLSSPGNPGAELCGAEFIAQLPGVMVEEEAQWVAQN"
     gene            19794..20477
                     /locus_tag="VFC01_13895"
     CDS             19794..20477
                     /locus_tag="VFC01_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN80758.1"
                     /translation="MGGSELTVLVVDDDFRVANMHAGIVNALPGFTVTGTATTLAAAH
                     KADPVDLALVDVYLPDGSGIDFVRELQCDSMVLSAATDAPTIRAAVAAGALSYLVKPF
                     APADLAARLSGYARYRKILAGTNLGGPEVDSALDALRPRIAPPQSPTAVASPTKQLVL
                     QALRAAGQPMSSAELSAEIGVSRATAQRYLSTLANSGEVKIQLRYGTTGRPEQEFVAV
                     DKPAGRPPR"
     gene            20702..>21003
                     /locus_tag="VFC01_13900"
     CDS             20702..>21003
                     /locus_tag="VFC01_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZN80759.1"
                     /translation="MIGLYALKPWYTRRLTPILNTAVAHNVSPDVFTAAGVVAAGAAG
                     VTIALGWWPLAALFLTLRLAGANLDGAVARARGVSRPWGFVVNEIGDRTSDLLTFAG"
BASE COUNT         3849 a         6697 c         6714 g         3743 t
ORIGIN      
        1 ctgggccgct tccccgagct ggagcttgtc ggcgatgtcg atcggatgcg cgcgacgatg
       61 gtgcccggcg tcaagcgaat gcccgtgcga ctcggaacgg caagctgagc atgtacctgg
      121 acttcacacc ggagcagaaa cagctgcgca cggagatccg ctcctcgctg gagacggtga
      181 tgacgccgga gcgcagtgcc gcggtggccg gtcgcatgga aggtggcgac gcggtcaagg
      241 agtgtgtgcg cgcactcggc gccgcacacc tgctcggcgt cggctggccg aaagaatatg
      301 ggggacgggg ttttaccgcg ctcgagcagt tcatcttctt cgaagaggcg cagcgggtga
      361 atgcgcccat cccacttgtc acgctcaaca ccgtcgggcc cacgctcgcc gactgcggta
      421 ccgacgaaca gaagcagaag ttcctgcccg cgatcctcaa cgggaccgtc gagttcgcga
      481 tcggctattc ggaaccagat gcgggtagcg acctggcgtc actgcggacc accgcggtgc
      541 gcgacggcga cggctacgtc atcaacggcc agaagatgtt caccagtggc gcggcgtacg
      601 ccgactacat ctggctggcc gcgcgcaccg atcccactgt caagaaacac aagggcatct
      661 cgatcttcat cgtgccaaca tcctcgcccg ggttctcctg gaagccgctg cacaccatgc
      721 ccggtgtgtc caccttctac acgttctacg acgacgtgcg cgttccggcc agctcgattg
      781 tcgccggtgt aaacgaaggc tggcggctga tcaccacgca gttgaacttc gagcgcgccg
      841 cgctgggcaa catgggcgcg ctcgagccgc tgttcgagaa gacactggac tgggcgagga
      901 cgaccgaact cgacggcggc cgtgtcatcg acaagccgtg ggtgcaacag gcaatggctc
      961 gagtcgaggc gcaggtcgcc gcctacaaga tcatgaatct gcgtgtcaac gctgcgatga
     1021 ccaagggtgc gctcggcatg ggcgaagcgt ccgcggtcaa ggttttcggc acggagctga
     1081 cgcagcgggt ggcgcgcgag ttgctcgagg tgcttgacgg tgccggcacg cgcaaaggcg
     1141 aggacgcgcc gttgcgtggc gctctcgagt cggcgtatcg gatcgccgtg atcaacacct
     1201 tcggcggcgg cgccaacgaa ctccagcgcg acatcatcgc aatggcaggg cttttcatgc
     1261 cgcgcgcacc acgtgatctt cgagcctcac agattgggaa taacaagtga ctgacgagcg
     1321 cttccgcgtg ggggcacctc ccgcccgaag ggcaggggga gaaacgacaa gcacggataa
     1381 ggactttcga gacaagctcg acgccctcat cggtcagccg accggtgggt ccggcaagcc
     1441 gaccgtcgcg ccggatccgg tgaaccagcc gatgattcgg cattgggccc acgcactcag
     1501 cgatatgaac ccgatctacc tcgaccccga gttcgccgcg aagtcgaggt tcggcggcat
     1561 cgtgtcgcct ccggtgatgc ttcaggcctg gacgatgccc gccccgaaga tcgaagggat
     1621 cgccgaacgc ggtggtgttc ccatggagat gggcaagaac ccggcggcgt tcatcgagga
     1681 agccggtttc accggcatcg tggcgaccaa ctcggagttc gagatcgagc gctatccgcg
     1741 tctcggcgac atcatttcgg tgacgcaggt gtttgaggac atctccgacg agaagaaaac
     1801 atcgctgggc accggccatt tcatcacgtg gttggcgacg tacaccgacc agaacggtga
     1861 ggtcctgggg cgacaacggt tccgggtctt ccgattcaag gccggggcga gctgatggcg
     1921 acgcgactgg caccgacggt cagcccggac gctgagttct tctggaacgg gctgcgcgac
     1981 cacaagctgt tgatccaacg atgcacgggg tgccaggcac tgcgtcagcc accgcgcccc
     2041 atgtgcccga cttgtaactc actcgactgg gacaccgtag atgccactgg ccgtggcact
     2101 gtgtacagct acgtgatgcc gcagcatccc ccgatgccgt tgatggagta cccctacatc
     2161 gtcgcgcttg tcgaactcga cgagggtgtg cggctggtat cgaaccttcg cgacattgcg
     2221 cccgccgacg tcgaagtcgg catgcccgtc gaggtcttct acgagacgtt cgacaccatc
     2281 gacgggaaag acatcgtgct gcaccagttc cgcccggccc gataggcgaa gcgcatggac
     2341 ttcaccttca ccgaggaaca agagacgatc gccaaggtcg ctcgtcagct gtttgaacat
     2401 cgagccaccc ccgaacacct caccgagctg gaagcgggcg acgtgcgcta cgacgctgac
     2461 ctatggcgcg agctcgcgac tgcggacctc cttggaattg cgttgcccga gaacgtgggc
     2521 ggcagcggcc acggtttcct cgagctggcc ctgcttctgg gcgaggtcgg atggagcgtc
     2581 gcgccggtac cggtctatgc caccctccta ctcggcgccg acgccatcgc ccgccacggc
     2641 gacgaaggcc aacggcagcg ctacctgccc gacgtggtca acggggccag gctgctcacc
     2701 gcgggtttag cggaacctgg ccgctcggac cccactgcgc ctgcgaccac cgcccgccga
     2761 gacggcggcg gctggcgcat tgagggaagc aaggatctgg tcccagcggc acaggtcgcc
     2821 cacacgatgc tgattcccgc cacagacgac gacggtgacg tcggcctgtt tctcatcgac
     2881 gtggacgccg gcggcgtcga gattcggccc gcgacaacca cgaacggcga gccacacgcc
     2941 gacgtattcc tcgacggggc agtcgtttcc ggccaggacc ggctcaacgc caacggtgcc
     3001 gagatgatcg cgtcgctata caaccgcgcg ctcgtcgggc tgtgtgccat ccaactgggc
     3061 gtcaccgagc gtgcgctgcg catcgcggcg gcgtacacga ccgaacgcga gcagttcggc
     3121 aggccgatcg gaagcttcca agccgtgcag cagcgaatgg cggacgcatt catcgacgtc
     3181 gaggcgatcc gctggaccat gtggcaggcc gcctggctgc tcggggaggg caagccggcg
     3241 tcacgggagg ccagcatcgc gaagttctgg gcgggcgagg cgggtgcgcg ggtcgcggca
     3301 actgcccaac aagtgcacgg gggcatcggc atcgacacca cctatcccct gttccgctac
     3361 ttcttgtggg ccaaacacaa cgagctgacg ctgggttcgg cgtcggctca gttggcgcgg
     3421 ctcggcgcgg cgtactgacg aaggagaaca atgacgacag acaaacttcg caccacgacg
     3481 ttgggctggc gcgacatcaa cgtcggcgac gaggtcactc cgctcgagat tccggtcacg
     3541 gcaaccgtga tcgtcgcggg cgcgatcgca tcgcgtgact tcatgccggt gcaccacgat
     3601 cccgagttcg ccaagaagca ggggtcaccc aatatattca tgaacatcct caccaccaac
     3661 gggctgtgcg tccggttcct caccgactgg gcagggcccg aggcgatggt gaccaaactg
     3721 gcaatccggt tgggcgtgcc cgcgtttccc aacgatccgc tgacgttcac cggcagtgtc
     3781 accggacagt ccgaaggcgt cgacggagag gggttcgtcg aggtcacctt caagggcagt
     3841 aacagtctcg gggaccacgt gtcgggcacc gccaccctga gcctcctcgc cgaggcctca
     3901 tgagcctcgg cggaaaggcg gccattgcag gcatcggcca gacggagttc tccaaggagt
     3961 ccggtcgcag cgagctccaa ctcgcctgcg aggccgttaa tgccgcgatc gacgacgccg
     4021 gactgcgacc cagcgacgtc gacggcatgg tcaccttcac aatggattcg agtgacgaaa
     4081 tagagattgc tcgaaacgtc ggcatcggcg acctgacgtt cttcacccgc gtgcaccacg
     4141 gcggcggagc ggcggcgggc accgtgctac aggccgcgat ggcggtggcg acaggcacag
     4201 cggacgctgt cgtgtgctat cgcgctttca acgagcggtc cgggttccgg ttcggcggcg
     4261 cgacggcgcg cccgaccgcc gaaaccccgc tgttcatggc gaactacgcg ccgttcggac
     4321 tgctgacgcc cgccgcatgg gtcgcattgc acgcgcagcg ctacatgtcg acatacggaa
     4381 tcaccaacga ggacttcggg gtgatctccg tcgtggaccg cgcccacgcg gctcgcaacc
     4441 ccgacgcgtg gttctacgag cggccgatca cactccagga ccaccagaac tcacggtggg
     4501 tcatcgaacc cgtgttgcga ctcctcgatt gctgccagga gagcgacggt ggcgtcgcgt
     4561 tggtcattac gagcgcggaa cgtgcacgtg acctcaggca ccccccggcg gtgatcaccg
     4621 ccgctgtgca aggtgccgcc gccgaaggcg agatgatgac gagctactac cgcgacgaca
     4681 tcaccggcct gccggagatg ggtgtggtcg ccgaaaagct ctggcgtgat tcgggactca
     4741 agcccggcga catccagacc gcgttcctct acgaccactt cactccgttc gtgttcgcgc
     4801 agctcgagga actggggttc tgcggccgcg gcgaggccaa ggacttcgcg accgtcgaga
     4861 ggctatcgct cggcggggaa ctgccgatca acaccaacgg cgggctgctc ggtgaggcgt
     4921 acatccacgg tatgaatggc atcaccgaag gcgttcgtca ggtgcgcgga acatcttgca
     4981 accaagtcga agacgttgag cacgtgctgg ttacctccgg aaccggggta cccacaagtg
     5041 cactgatcct cgcgcccgcc gtctgaggat gatgtgacta ccgaaacgac cgccgccgac
     5101 acgcgcgagc tcatcatcga gtccgcattc ggttgcttcc gcaggcaagg gctggacaag
     5161 acgaccatcg tcgatatcgc gcgggcagcc gacatctcgc gcagcaccgt ctacgagtac
     5221 ttccgcgaca agtccgagat cgttgaagca tgcgcagagc attcgtcgca gcggttctac
     5281 cgcgagatgg ccaaggcgat gggtccgggc gaatcgctcg aggagaagct gacgcaggcg
     5341 gcggtgttcg tggccagggc ccggcgattc attgagcccg aaatgtactt tgacgcagac
     5401 gaagtgagcc tgctgcttac gaagaacgcc gcggtgctgc tgcgcgagtg ctgcgagttt
     5461 ctggcgcctt atctcacggc ggcaaagctc accggcgagg tgcgcaagga tctagacgtg
     5521 atggccgccg gcgagtggtt cgcacgcatg ctgttctcgt tgttcagcac accgtcatcg
     5581 acgctgaaca tggacgatga cgcgattgtc gctgactttg tgcgatcgca tgtcgtgcgg
     5641 ggattcgcga acgaccgcgc cgggcgctcg cgatgaggct gcgactaccg gagacggcga
     5701 taccagcttg tgttcatgta aatactcctg tggggattgt gaagaactgc ggcggatcag
     5761 tgtgcgacca gctcagtaga ttcccattgc atcgtcgcgt tgagtgaaaa agcggcaaat
     5821 gtgctcgaaa gtgtctgccg cagttcgaca tccgagttgg ggcgacccgg ctgatacgac
     5881 agcaccgaga tgccgacctt gccgttgacc cgtcccgaaa tgaagcccag gaccccgccc
     5941 gtgcggtgca tcgtcgccct ggtcatcccc gggtagacgg accgcgccgt gcagtactcg
     6001 gcgtcggtgc cgtccggccg ggtcaccgcc gaggggacct caccgaggtt ggacgacacc
     6061 acgcttgctg cattgccggt agcgacgctg accatcctcc tcatcaggcg cttgggcaca
     6121 aggggaacga tcggcaacag tgccaggcgc tcgtcgggaa cgttgttgtg gtagatcagc
     6181 gcctgcttga tggcggcacg gatcccgcgc agatcggtgg tcgcgtccgt gggatcgact
     6241 gtgacgtcga cgttcgtcac cgcgttggca cgcgtgtcgc cgtcggttcg ctcgttgacc
     6301 ggaatcgaca gcatcacagc gtcgcccccg acggttaccc gccccatccg ctgtgcggcg
     6361 tcggcggcca gacccgcaag cagcgcgttg ctggtgccgc cgagtgactg ggcgcgagca
     6421 tcccattcgc tcgcatcgat aaacgccgtc gcacccggca gcgtgatccg ttcatcacga
     6481 gaatcaacat gtttcgatgc gcgtgccggg gcgccggcat cacggcgatg gcgccgcgcc
     6541 atccggatgg ctgcccgcgc ggcgcggccg acggcaggaa tatcgcgcac ggtctgacgg
     6601 gcgtcctgac ccagtgcccg ccagcggcga cgtgacctcg cgggaggcca catgatcgcg
     6661 tcgtcaacgc cggaggtcgc ctcgaccagc gccatggcca gtccgactcc gtcgatgagg
     6721 gcgtgagcga cgaccaggct caccccggtt ccgccgtcgg tgaacggcac aaccgcaagg
     6781 tgccatccgg ggccgtgttc ggcgtcgagg ggcgtgtggg cctggttggt gagccaatcg
     6841 tcgaattcct cccgcggacg gggtgtttgg gtgaactcga tgtcggacga gtcgctgctc
     6901 gtcacccagc gatggcgacc gaaaggcaaa ggcgaccgtt cgatgcgacg ggccagccgc
     6961 ccccgctgaa gatggtggtg gaagcgccgc agaccatcga catcgatcgc ccggtcgtag
     7021 acccagatgc actgaatcac gctggtggct ccggttgcgc gctcgcccag gaacaatgcc
     7081 tggtcggcca gatcgagaat gttcgtcatt tcttcggttc ccttcatttt cgtcatttcc
     7141 gtcaccggct cccggcgcac acggcgcacg agtcactgga attcgcatgg tgggcaagct
     7201 cttcgatcca gtccgcggtg atctttgcga cgtccgcgaa cgccttgctc agcgccgcga
     7261 tgtagcggtg caccgatttg cgcgcaatcg ggttgtcggg gaaggagatt gtcaccgtgg
     7321 tgcggccggc gtgccggttg atccacatgt tgattccacc gtgcgacagg ttgtcgccgt
     7381 aaatcccgta attggtctgc tcccacaatt cggcgacggg gatcttgcgg aagtcgagga
     7441 aggacaccat cattcccgga cgggtaggca gcttgatccc gagttgatcc accggcgcaa
     7501 gttcgagaac gcgctcgaac ggcacactgg ccaggcactt gcccgcgttg aacgacttct
     7561 gcgctgcgcg agcgatttcg gcgaactcac cgtcgccgat cggcacggcg acggggaaga
     7621 ggctggcgaa ccatccgacg ctcatggtgt caatgtcggc cgtacgcgta tccgacggag
     7681 tgaatccgtg ataggtggtg ttgccggtga gttggtgctc ggccaacgcc gcgcacgcca
     7741 ggacaccgcc ggagaaccgc gcgccggcct cgcgacaagc ggtgtcgaaa gcctccgtgt
     7801 cgtcttcgtt caacaactcg acggtgacga agtcgccctt gttgtttgcc cacgtgtcgc
     7861 ccagctccag cggaaagctc ggccagtcgc cgtcggtgct ctgggcgaac gcgatccagt
     7921 ctttgatctc cggcgacgac agggtcatgc tggcgacctt ctcgcgctga cgggcggtgt
     7981 agtcgcggta cccgccgacc tccggaagtg tgacaggctg cccctgcgac aagttctgat
     8041 acatcaggtg caggtcgacg aaaatcagcc ccgccgacat gccgtcgatg tgcagatggt
     8101 cgacgctggc gtacacggtg aaatggtcag cgctctgaat gacgccgaag ctgaagcagt
     8161 cccattcgag ggtctccgat gtcatcgcca gtgcgtgtgc gcggatctgt tcttgctcca
     8221 tactgccgaa cgaagagggc acgaattcga tttccttggg gtcgtcaatt gtgcggcgaa
     8281 aaatgtttcc attctcgaat tcgaacgtgc tgtgataggt gtcatggcgc cgcacgtgcg
     8341 tgttgatcgc ctcggtcatc agcgcgatgt cgcactcacc ggggatatcc cacgccacga
     8401 ccatcagccg gggtagctct cgcccgagcg cctttccgta gaaggcgccg cgcaggtgct
     8461 gagcctgctg aaagctcgga ggcagatcgc tccgactcgc cctactggcg ctttcccgcg
     8521 cggccgaaga agccgtccac gtagtaactg tgccggctgc cgggtgccat gaatggatgg
     8581 ggccgagcga gaccatttct aagctccttt gattcggtgg tgcgttgact cgatgcgacc
     8641 gaacgtaagg tgccgccgac ggaatgactc gcttgtgctc gctagtcgcg aagtgagcga
     8701 tttaaccggt tctgcgcatt gtgctcaccc gtcttacgag gtgattcgtg cgcttgatat
     8761 gcgtgagcac aacgcgcaaa agccgaggaa tcgccgcttg tgcgggttgt gcgcacaagc
     8821 gcgccacgac tgggccgcat ccgtaatttc agcgctgccc gcagtacgaa ctcaacaacg
     8881 aaggacccgc cgtgctaaag aaagccacca ccaagacccc gaaaaacttc gacggtttct
     8941 cgctgttggg tcgcgccgtg gtgcgcgctc cctggctggt catctccgca tggatcacct
     9001 tggtcgtcgt gctgttcatg gcctttcccg cgctgacgaa ggtcgtcgag aaccaaacga
     9061 ttcaaccgct accgccgcag gcgatggcgg ccaccgacca gatggccaag gattttcacg
     9121 agtccgcgca aaacattctc gtcgtggtca tgaccaacga gcatgggctg aaccccgccg
     9181 acaacaacac ctaccgcgcg gtggccgaca aactgcgcag tgacaccagt gacgtgtccg
     9241 ctgtgcagga tttcatcagc acgcctccgt tgcggcagtt gatggtcagc aaggacaaca
     9301 aggccttcta catggcggtg actctcaagg cgccgcccgg ctcgccggag tcctcgcagg
     9361 cgtatcaaca gatcacgcag atggtcaagc aagccaccgc cggctccgac ctcactacac
     9421 acgtgactgg atcggcggcg gtcatcggtg acctatcgat cgtgaccgct cacgacatgc
     9481 atgtgatcga gatcgccacc gccgcactgg ttctgctcat cttgctggtg atctaccggc
     9541 gtcccgtcac cgtgttgctt cccctgatca ccatcggaat ctcagtcgca tcagcccagg
     9601 ggttggtgtc tgcactgact gagatcggga tgagcgtttc ggctcttacg atcgtgttga
     9661 tgacggcgat gatcgtcgga gcaggcacgg actacgccgt cttcctgatc agccgctatc
     9721 acgagtacat ccggtccgga atggactccg acgcggctgt ggaaaaagcg ctcacctcga
     9781 tcggcaaggt gatcggcgcg tcggcggcca cggttgccgt cacgttcctc ggcatgatct
     9841 tcacccaact ccccgcgttc acgagtgtgg gtccggcgct ggcagtttcg atcgcggtgg
     9901 cattcctcgc ggctgtgacg ctgttgcccg ccgttctggt gctggcgggc cggcgaggtt
     9961 ggatcgcacc gcggcgaccg ctcaccgacc gactgtggca gcgctcggcg gttcacatcg
    10021 tccggaagcc gaagtcacat ctatttgtca gcttgaccat attgatcgca ctcggggctt
    10081 gcgcattgtt catgaacccg acgtacaacg accgcatgca gctgcccgga tcggcagaga
    10141 gcaatctcgg ctattccgct atggcagacc acttctcgac gagcgccttg ctgccggagt
    10201 acatctacat ccactcgccg cacgacctga ggaacccgca gtcgctggcc gatatggaac
    10261 agatggcgca gcgtgtggct cagctgccca acgtcgcggc agtgcgcggt gtcacccggc
    10321 cgacgggcca gccgattgag cagaccaagg tcagctatca agctggtgag gtcggctcca
    10381 aacttgaaga tgcgtcgtcg cagatctcgg ataaatcaag cgatctcgac aagctgagcg
    10441 gcggcgccca ccagttggcc ggcagcctcg cccaagttcg tgaccaggtc aacggcgccg
    10501 gccgatctat gacggcgatg acggcgacac tcagccaggt gcagaatcag ctggacggac
    10561 ctcaaacgac ccaaatgctg aacaccattc gtacctatgc gaataccgtg ggtggcaatc
    10621 aggaggcgat gaacggggtc gcgaactccg cgcctgccat gctgaacgca ctgagcaaca
    10681 acccgcagtg cgacgccgat ccggtgtgca gcgacggccg cgccaagctg cagcaactcg
    10741 cttcaacggg ctacagccaa accggggcgt ctgcgcagag catactgtcc tcggtgcagc
    10801 ggctgagctc gatgatgcaa tcggccggca ccagtctgcg gtccgccggg atcaacagtc
    10861 cccaaggcgc acaagccaag attgcgcaga tgcaacaggg cgccaacgcg ttggccaatg
    10921 gcagcgcgca actggcccaa ggcgttcagg tgctcgtcga tcaaaccaag aagatgggtg
    10981 gtggactgaa ccaggcggcc agtctgctgc tgtcgatcaa gcacgacgcc tcgtcgccgt
    11041 cgatggcggg tatgtacgtt ccgccgcagg ttctgacgac gaacgacttc aagaacgccg
    11101 cgaaggtgtt catctcgccg gacgggcatt ccgcccgcta catggtcgag acgaagttcg
    11161 acccgttcag cacggacgcc atggaccagg tgcagacgat cctcgatacg gcgcgcggcg
    11221 cacagccgaa cacgacgctg tccgatgcga cgatctcgat ggtcggtacc actccgatgt
    11281 acagcgcgat tcgcagctac tacgaccatg accttcggtt gatcatcgtc ttgacgctgg
    11341 tcgtcgtctt cctcatcctg gtcgccctgc tccgcgccat cgttgcaccg ctgtatctga
    11401 ttgcctccgt ggtgatctcg tacctgtcgg cgttgggact aggcgttgcc ttcttccagt
    11461 tcatctgcca tcagcacatt tactggaatg tgccggccac tgccttcatc gtcttggtcg
    11521 cggtcggtgc ggactacaac ctgttgctga tcagccgcat ccgggaagag tcccgtaatg
    11581 gcatccgctc cgggatcatt cgcgccgtcc attcgaccgg cggtgtgatc acctcggccg
    11641 gcatcatctt cgcggcctcg atgttcggcc tgatgttcgg cagtgtctcg acgatgctgc
    11701 agatcggttt catcatcggc gcaggcctgt tgatcgacac gttcgtggtg cgcaccatca
    11761 cggtgcctgc actggccgcg ctgatcgggc gggcgaactg gtggccaacg aagtcgcgca
    11821 aagccgcgcc cggccgcaag gcggtggatg cccatgccgg gtaaccgatc tgatgttccg
    11881 cagtagttac cggagggccg ccaattggcg gccctccgtc actgctgcta gcgatatgtg
    11941 tgcgtttcgg agtgctcacc gctctggcct ggccccgatg ggttgatcca cggcttgtga
    12001 gagtcttgcg gcccacgacg tgggcaggaa ggctcaacaa atcgatgtgc atcggaatgc
    12061 gacccgtcgg ataaccgatg agtttctcat gccacctcgg tcctcttaga tacaaccgaa
    12121 ctgaccacac ttggatggag gctatgtgac cagaaccaat ctttcgatgt cgatctctgc
    12181 cgacggctat gttgccgggc ccggccagtg cgaggagcag ccgctcggag tcggcggcag
    12241 gacgcttcat gactggcacg taggccccac caagaaccat cctgtcaacc ggcaggtggt
    12301 ctccgacatg atggacggca tgggagcgac gatcatgggc cgcaacatgt tcggcccaat
    12361 tcgtggcgaa tgggaggact cggactggaa gggctggtgg ggcgacgtcc cgccctacca
    12421 ctgcccagtg tttgtcctga ctcactaccc acacgacccg atcgagatgg agggtgggac
    12481 cacctttcac ttcgtcaccg acgggatcga gtcggcctac gcccaggctc aggcggctgc
    12541 tggtgaccac gccatttcca ttgcaggagg ggcgtcgtgc gcccggcagg cgatcgccgc
    12601 aggtctagtc gacgagatcg atcttcagat caacccggtg atcctcggtt cgggtgagcg
    12661 cctgttcgac gggttcgagg cgggcaagcc cgcacttgag ctagtccggg tactcgaagc
    12721 cccgggcgtg gcccacctgc gctaccgcgt cgtccgatga cccggtcgtc gtgttagtca
    12781 tggaaccggt gtcttaacct gttgcggcac ctcggtagtc cacccggtac gctgctgcct
    12841 gcactcaccg atgaacgact ctcatccgcc gtggaccaat tcttggggtc cggtcagctc
    12901 ttcatcgcac acgaatcgct tgactgccag tcatcgccag aggaacctag cggcccatga
    12961 gcatcgctgt gttgcgtcag atgttcgagc agatggtcga gaacaaagac ccacagccat
    13021 cgatcgcttc taccatccgt cattcgtgat gttctccaac ggcgtcaccc aggatttcga
    13081 agcattcgcc gcaagccacc gcaccattta cgccacgccg atcagctatt ccgtggagta
    13141 cgacgaagag gcctgggtag agacgaccga caaggtcgcc ggccgggtct ggatcaccac
    13201 cagccgcccg ggcgagagcc caactcgtat cgaagtcgtg ttgatcgccg cttttaccga
    13261 cggccgcatc agccgtgtgt gggaaacaac gtggccgagc tggaacacat tgccagcatt
    13321 cgacacatac tgattgcgtg aatgccttca gcagccgtgt ctaactagct ccgaatgttt
    13381 tcgcaagcgc atcgtcgagc ggatacggcc cttcgggagg taagaggaac catgcgcgcc
    13441 taggcagcgc ggggcgtgtc ctcggcagtt gtgacaacga aatcgtcgat caggcgtgcc
    13501 acagtctcgg gacgttccgt cggcggatgg tgctggacac ccggcatgat gtgcagccga
    13561 ctgttcggca gcattcggtg cgctgcacgt gcgtgctcga cggggatgac ccgatcctgg
    13621 tcaccgctga tgatcagggc gggcagaacc ccgtgaaagc ccaagcgatt cagggcggac
    13681 accgcctgac cgcgaaagtc gacaaccgag cgcagtgtgc gcaggaacgc ttgcctgttg
    13741 tcccggttcg acagcgaggg acgagctttg aagcgatcgg aggatcccat cagcgcccgc
    13801 agagcattgc cggcgaggac agcaggccgc gacgcgatca ccggcaacac gagctcggat
    13861 ccggggaggg acagcaggcg cagcacacgg ccgacatcgt cgccaaaccc gccgctgctg
    13921 atcagcacga tccgctcgca gtactggcgg tgttgatggg cgaattgcat ggcgaccccg
    13981 ccacccagag agtgaccgat gaccgtgact cgggggacat cgagcgcgtc cagtagatcg
    14041 cgaagcagga cggcgaaggc gcccacagaa taatcgccgc gcggcttgtc ggattcccca
    14101 tggccgagca ggtccggtgc gatgacgcga tacttcttgg cgagcagcgg tattacgccg
    14161 ctccaggtat tggaactgcc acccatgccg tgcaccagca acaggacctc accggcgccc
    14221 tcatcgcggt acgcgacgcg gtcaccgtgc aacgtcatgg tcttgaggtt cgcggtggcg
    14281 tcgctgttgg cggaatccgg gtgcgcgtca cacctgggca gtgagacgac ggccggggcc
    14341 gcgactaagg ctgcgcaggc agcggcgccg accaacagac tcttcatggc attcatggcg
    14401 tttgctccgg ttgtactgtc cccgttgggg acaactatcg ttgacgatca ctaccggata
    14461 cgttatggct tgcgtaccag cacttctcgc ttgtgctcac tactcactac aagcagcaat
    14521 gtccgcgttt ttctcataat gctcaggggc gtagagggtt gctgttgact ctgcgcacag
    14581 ggcgaaaaag tgcgagtgga ctaaaccgcc accgcagagt caacggagaa atcaggacac
    14641 cacgactgcg agttgcgctg cggaggaacg gttccgggac ttgcgcgcgg tcgcgggacg
    14701 gcgggatccg cggcgcatgc ccatcgctcg gccgatcgca tcgctgacac atgcggcagc
    14761 ttcctgaagc agcatgtgac cggcagtggg gtggtgcaga tgcaccgcac ccgggatggc
    14821 ggcggccaag tcgtgcgagt gggatgccgg agtaaggatg tctgtcccgc cgctgacgac
    14881 gaacgtcttg gcggaaatcg cctcaagcgc ttggtacacg tcgtagcgct tcaggctcgg
    14941 caggaagccg gccgccgttg tcaacgacgt tgtgcggatg gcggtcgccg cgaccgccgc
    15001 taccgcggtg ccgtcgctgg agaatctgcc caacgcttcg ccaagcggcc ggatgagcgc
    15061 ctggacgacc ttatcggtcg ctcgttgcgg catgcggtga acgagttcga acagcgctcc
    15121 cgtcgcgggc gtcgccagca ggcgaccgat gccccgcgcg gcgagcctgc ctgcggcggt
    15181 gccaaccaga acgaggcctc gaggttccac aggacgcttc gacgccgggc gcccgaaata
    15241 cgccagcgcc gtcatgccgc ccatcgagtg acccgcgagc gtgagcggac ccgtgatgtg
    15301 caatgcggtc aggacctccg cgagatcggc tgcgagttgc gcgatttcgt acgtgggcat
    15361 cggcgagccg gtcgaccggc cgtggccgcg atggtcgtag gtgacaatgc gaattctgct
    15421 gccataccga cgccgcagtt ggcgcacctg gggctcccag ctctcttgcg agagtaaaag
    15481 accgtgcagc agaacaactg ttggcgcgtc ggctcgatca gagctgtagt cggacactgc
    15541 cagacggacg ccgtccttcg tcgtcaccgt ccgctcgacg cagccgtcac cggttagtgc
    15601 ggcaggacga agcacggcgg gtgccagacc cgaatcgggc gcaaccgaca tggtggactc
    15661 ctcggtaggt gccgatgtcg ggacgttccc gacaacctga tgcgcccgaa gctacggatc
    15721 ggcggaggcg gtggctcgct tgtgcgcaca acccgcgcaa ccgggcgatt ttgagctttt
    15781 gcgcattgcg ctcaacagac gcgcatacga aagctcctgt gctgactaat gattcgaatg
    15841 atccgaggag tcttgagcac aatgcgcaaa aggcaacaaa acgtctattg cgcgactagt
    15901 gcgcacaacc gagccaacgc gcgagcaact tcatagcgtc gtgggcagtg acgagagcgc
    15961 aaggagcaag agatgacgac ctggccgtca aaccatagcc acgaccagtg gtccgcagcg
    16021 accatggcgg cgtgcattcc acagaccgtg tcgatgcgcg cggcggcgga attcaacgca
    16081 acaccaacgg ccgcgcgtcc ctacatggcc gacctggcgt ggtcggccga gccgcccggg
    16141 tacgcttcgc cgctccccat ccagcggcgg cggcgctggt tcacgcgttc acgtgtgctg
    16201 acttttgtcg gcggcggtct tgtggccgcc gcggcagccg gcctcttcgg agcgctgctt
    16261 ggcgcacaaa gcactccagt cgacgtgacc cgccacagcg cgcctccagc tgcgcctgtt
    16321 gccgcaccgg cgaatccagc cccgacgccg ggtatgcaga cgatgccggc cacgacgaac
    16381 cactcagtga tcgccagcca ccgggcatcc tcccattccg ccggccatca ctcgccgcct
    16441 ccgccgcagc aatactcgac gccgccctcg tcagggcaga acaccaatat ccagcagagg
    16501 ccgtgcgatc acgacacggt gtcaacgccg ccgacatgga atcgaaactt cttctactgg
    16561 ttgacgcatc gacgcgacca cgacggtagc cgttggacac acgatcagag cagcgacaac
    16621 cactcgacgg gctcaggcga caacgggcat tcgaactaac gacatgaccg cgacattgcc
    16681 cgccgaccag cgggatatcg ggcggcgact cgatcacctt cccgtcggcc ccgtccaccg
    16741 cagagtggtt atcgcggtcg ggctgggcct gttcttcgag gtgtacgaga tcttcttgtc
    16801 cagcacgatc gccacagcac tgtcgacgca atacggcctc ggcggcaccg cgctacagct
    16861 gctgctcgcg tcgtcattcc tcggaatgtt cttcggtgca gcggcattcg gtcgcatcgc
    16921 cgatcgcatc gggcgccgac gggcgttctt gttgaatctc gtgtggttct ccgtgtggag
    16981 cgtcatcgga gccgtcgcgc cgagtccgtg gatcctggtc ggggcacgct tcatggcggg
    17041 gataggcgtt ggcgccgaat atcccgtcgc cgacgcttac ctgtccgacg tcctgccgat
    17101 ggctcatcgc ggacggctag ccgcctgggc ctacacctgc tccttcctcg cggttccagt
    17161 gctggggttc ctgtcgctgt cactcaccgg gcgcagccta ttcggcgtgg agggttggcg
    17221 ggtcctgttg ttggtcggcg cactcggcgc actgttcgtc atcgtgctgc ggcgcggcct
    17281 gccggagtca ccacggtggc tggcaagcgt ggaccgcatc gctgatgcgc gggccgcatt
    17341 gcgggcgttc gaatccggta cgggcccggc accggagccc gccgctgaca gcgaagggga
    17401 cgatgacgtc gcggccgcct ccgagccggg tcggaccgtg tctgcgcttc aacgactacg
    17461 gctttcgccc tatcggcagc ggctgatcat gcttggggtg tttcatctgt tccaaccctt
    17521 cggctattac ggattcggca ccctggctgc gctggtattg gtcagccgcg gctacgacgt
    17581 gacggcatcg ctgctgttta ccgcggtgtc gttcatcggc tatccgatcg ggtcgctggt
    17641 ttcgattccg ctgctgaaga ggttcgaacg gaagaacctg atcatcgtca tggtcgccgt
    17701 gatggcgaca gctggcgtcc ttttcgcgac cgtgaacgtc cccgcgctca ttgtcgtatt
    17761 cggcctcctg atcacggcgg cgagcaacat cttctcgaac atctaccacg tgtaccaggc
    17821 ggagatcttt cccagcgatg tgcgggcaac cgccgtcggt tggacctatt cggtttcgcg
    17881 gctgtcgagt gctgcgcttc cgttcattct catcccggtg ctcgataact acggcgccac
    17941 agcgatgttc gccgccgtgc tggtcgcgct cgccgccacg atcgccgtga ttgcacccat
    18001 cggaccgcgc accacgggga agagcctcga cgaaatcaac ccgacatgac cgccgcgcaa
    18061 accaacgatg tccacattcc gagttttgcg cattatgctc aggaagccgc gtacgctctt
    18121 tccatggtga gaaggctgca cgtagatgtg cggctcgcta cccaggtgct gttgttgcaa
    18181 gtcgccgtgg tgacactcac gctgggcata gcgggcgggc tggttgcctt catgagccac
    18241 cagcgccttg ccgcccaata cgaaacccgg tcgttggaca tggcgcgtgc gatcgccttc
    18301 gcgcccgcgg tccgtgccga cgtcgcacgc tacgacaacg ccccgttgac gccgagcccg
    18361 gcgctcaccg acgagctggc gaacgggcag ctgcagcaat tggccaccga gatccagcag
    18421 cgcacaggcg tgttgttcgt ggtgatcacc aacaaccagg gcatcaggct gacgcacccc
    18481 aaccgagacg aactcggcaa acatgtcagc accgatccct ccgaggcgct cgccggccac
    18541 gaggtggtga cgagggaaac cggcacccta ggaagatcgg tgcgcgcgaa agttcctgtg
    18601 ctggctcctg attcaaatcg ggtcataggc gaggtcagta tcggcatctc gaccgcggcg
    18661 gttcaccagc agctgtggac cgacgtgcgc accgccgcgg ttctggtggg cttggcgctt
    18721 ctcatcggtg tcgtgggctc cctgtttctg gcccggcgct ggcgaggcct gaccatgggg
    18781 ctgcagccct ccgagatggc cgaattgata cgcggtcagg cagcggtgct gcacggtatc
    18841 gacgagggtg tgctagcagt cgacaccgat tggaagacaa cgttcgtcaa cgacgaggcg
    18901 tgccgcctcc tcgaggtcag caacgaatcg ggcaggccag tggacgagat cgggttgacg
    18961 ccgcgggtgc tcgaggtgtt cagatcggcg gatccgacgc caacgcttgc caccgtgggt
    19021 gatcgggttg tggtggtctc ggcacgacac gtggcgcgtg acgaccggga tctgggaacc
    19081 gtactggtcg tgcgcgatcg caccgacgtc gaatcgctga cccgccaact cgacgccgtg
    19141 cagctgatga gcacggtgct gcgcgcccaa cgccacgagt tcgcgaaccg gctgcacctg
    19201 atcaacggtc tccttcacag cggccacacc gaggaggccg cccaatatgt cgaggagttg
    19261 ttgggatcgg gtccgctggg atcggcactg ccggggatcg acactgtccg cgatgcattc
    19321 ctgcaggcat ttctggcggc gaaggcggcc tccgcccgcg aagcaggagt gacgctgacg
    19381 atcggcgaga acacctgggt cccaggtcga ctcgcgctgc cggtcgacgt gacaaccgtg
    19441 ctgggcaatc tggtcgacaa cgccatcgac gcggcccgca caggcgccaa cgaagccaag
    19501 gtggtggaag tcgaactgct gcaggatggt tcgacgctgc acatcacggt ggccgacacc
    19561 ggggatggcg tcgcacccga cttcgtcgac gacgtgttca ccgaaggcaa gtcgacgaag
    19621 ccggagtccg ggatacccgg gggtcgcggc atcggtatgg cgctgtctcg acagatcagc
    19681 cgcgcactcg gtggtgatgt ccggctgtcc agcccgggca acccgggcgc ggagctgtgt
    19741 ggcgcagagt tcatcgccca actgccgggc gtgatggtgg aggaggaagc ccaatgggtg
    19801 gctcagaact gaccgtgctc gtggtcgacg acgacttccg agtcgccaac atgcacgcgg
    19861 gcatcgtcaa cgcgctaccg ggcttcacgg tgacggggac ggcgacgaca cttgccgcag
    19921 ctcataaggc cgaccccgtc gacctggccc tggtggacgt ctacctgccc gatgggtcgg
    19981 gcattgactt tgtccgcgag ttacagtgcg acagcatggt tttgagcgca gcaactgacg
    20041 ccccgacgat tcgcgccgcg gtggccgcgg gagcgttgag ctacctcgtc aagccgttcg
    20101 cccccgcaga tctcgcggcc cggctgtcgg gctatgcgcg gtaccgcaag atcctcgccg
    20161 gaaccaatct tggtggaccg gaggtggact cagcactgga cgccctgcgc ccccgcatcg
    20221 cacctccgca gtcgccgacc gccgtggcct cccccaccaa gcagctggtg ctgcaggcgc
    20281 ttcgcgcagc cggccagccg atgtcgtcgg ctgagctctc cgcggagatc ggcgtgtcga
    20341 gggcaaccgc gcaacgctac ttgtcgacgc tggccaattc cggcgaggtc aaaatccagc
    20401 tgcgctatgg cacaaccgga cggcccgaac aggaattcgt cgcggtcgac aagccggccg
    20461 gccggcctcc gcgctgacgc cacgatcggg tcgcggtggg ccgaccccat gatgcccgcc
    20521 gacacaggcg ccattcagca aatagtgtta gggagctgta ctgctgcttt tcaagcatag
    20581 ctaacacgaa tcagtccttg gatctgactg ccaagaatcc gccaaggatc ccccaaggcg
    20641 tcggtcgcaa tcttttcgtg accggcccca caggccgaac tgcaacgaaa ggaatgagca
    20701 catgatcggc ctgtacgcac tgaagccctg gtacaccaga aggcttactc cgattctcaa
    20761 caccgcggtg gcgcacaacg tgtcaccgga cgtcttcacc gccgccggag tggtggcggc
    20821 cggcgccgcc ggcgtgacaa tcgcgctggg atggtggccg ctggcggccc tgtttctcac
    20881 gctgcgactg gccggcgcga atctcgacgg cgcggtcgcc cgtgcccgcg gagtgagccg
    20941 gccgtggggt ttcgtggtca acgagatcgg cgaccgcacc tcggacctgc tgaccttcgc
    21001 cgg
//
LOCUS       DASRJA010000320        21255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2022949,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000320 DASRJA010000000
VERSION     DASRJA010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 21255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21255
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022949"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFC01_13905"
     CDS             <1..696
                     /locus_tag="VFC01_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80760.1"
                     /translation="PSDTRDRLLSDVASGAVRAAVALESTEFVPDAEGADRVLVIADG
                     GLAVASPAVTPQPVLDETRRLATVTADDGAEMLQFEGDHAAAVRRLLDRAAVAVACDS
                     LGLCEAMLSATVGYAKVRHQFGRPIGSFQAVKHACADMQVSIAVSRQLVSAAVEAVAA
                     ERPDAATAAAMAKSYVCSAAVDVVGKAMQLHGGIGYTWESGIHVYLKRAALNRSLFGS
                     PAAHRKRLAQRYL"
     gene            711..2417
                     /locus_tag="VFC01_13910"
     CDS             711..2417
                     /locus_tag="VFC01_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZN80761.1"
                     /translation="MGVPVYKRILDLFEAEGVNTLFGIPDPNFVHMFAEADARGWSVV
                     APHHELSAGFMAEAVSRMTGGPGLCIGTLGPGMANIAGAIQCAKVENSPVIFLGGQRA
                     RITERRVRRGRIQFVRQEPLFAPSVKYSASIEYADQTDEIIREAIRRAMSGTPGPSYV
                     EYPAHILLTELDVPDPLPPNRYRLVNQGAGAPEVAEAAKLIREAKSPILLVGHGVHTS
                     RTQSAVKELADLMACPVIQTSGGTSFIPGLEDRTFAYGFSPSAVDAVVNSDLCVALGT
                     ELGEPVHYGKTRHWAERNDIRKWIYVEQDPMAIGVNRQIDVPLVGDLRGVVPQLVEAL
                     KDTPRQPSPNLDVLIKKDAEELAQLAENAPTGRTPVHPARFVVEATKAFPKDGILVRD
                     GGATVIFQWTYSQAKPRDVIWNQNFGHLGTGLPYAVGASVAEGRKRPVMLLTSDSAFL
                     FHIAELETAAREGLPLVCVVGVDHQWGLEVGVYKRTFEQPSPQPGVHWSKDVRFDKIA
                     EGFGCHGEYVEKEDEIGPAIERAYASGKTAVVHVCIDPKANSEEMPKYDEFRTWYAEG
                     TQ"
     gene            2432..3868
                     /locus_tag="VFC01_13915"
     CDS             2432..3868
                     /locus_tag="VFC01_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN80762.1"
                     /translation="MREYLKFYIDGQWVDPVRPNTLDVDNPRTEQVSGKISIGSSADV
                     DVAVQAARRAFASWSQTSREERLDVLQAMLAEYQKRAGDLADAVSEEMGAPPSLAAGP
                     QVNLGIGHLATAIDVLKNFEFEEQHGATLVVKEPIGVCGLITPWNWPINQIAVKVYPA
                     LATGCTMVLKPSEVAPYSAQIFTEIVDAAGVPAGVYNMVFGDGPGVGSAISSHPDIDM
                     VSFTGSTRAGVEVARNAATTVKRVSQELGGKSPNIVLDDEDFAKSVTAGVSVMMMNSG
                     QSCNAPSRMLVPNSRMDEAVAVAQGVAGAVTIGDLNDKSAIGPVASKAQFDKIQGLIQ
                     KGIDEGATVAIGGTGRPDGLDTGYFVKPTVFAHVTNDMTIAREEIFGPVLCILGYDNL
                     DQALDIANDTEYGLAGYVSAADLDKARSVARRIRAGSVAINHAFDMNAPFGGYKRSGN
                     GREWGQFAFDEYLETKAALGYAPEATAG"
     gene            3875..4423
                     /locus_tag="VFC01_13920"
     CDS             3875..4423
                     /locus_tag="VFC01_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN80763.1"
                     /translation="MDASTAELTALLDRDKVRDCIARLARGEDRRDAGLISGSFWPDA
                     TIDLGVFAGSFDEYLAWVVPGSPLIPVTQHVLGQSVIELDGATAFAETHVTAYHRLTA
                     DDGDRDTVIGGRYLDRLDKRGAEWRIGQRTMLYDWYHDFGRSTDWAQGLMGLQFSTDH
                     YTGSADLDYSETFFGKGPKWER"
     gene            4411..5292
                     /locus_tag="VFC01_13925"
     CDS             4411..5292
                     /locus_tag="VFC01_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80764.1"
                     /translation="MGALTGKVAVVTGASRGIGKGVAVALGAQGVTVYVTGRTMTPGS
                     HPLPGTVGDTAKEVTRRGGQGVAVQVDHGDDAQVAALFDQIKSDHGRLDILVNNAFAL
                     PEDLTEPGEFWEKPLSHWDMVDVGVRSNYVAARHAATMMVPQGSGLIVAISGYAAVTY
                     TYGVIFGMCKTAVDRMARDMAVEFEPHNVASLSLWQGLTFTERAQRNLRRSPEMTKQT
                     VTDPAVGCSPEFPGRVIAALASDPDVMKRSGGTFITAEVAWDYGITDVDGKVVPSLRA
                     ERGEPVWRPIAEAGHGR"
     gene            5282..5815
                     /locus_tag="VFC01_13930"
     CDS             5282..5815
                     /locus_tag="VFC01_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN80765.1"
                     /translation="MDADRIALLVDRQDILDCLTRFSRGMDRFDRDVFLSAFHPDAVI
                     AAGDFVGGPADLFQWATAMHERGQIATHHNLLNHTCEIDGDTAHAETYFLFAARNRDD
                     TNWIAGGRYLDRLHRRDGAWRIAVRTNVIEWSGMVPTMPLPFAEVPDIHGNGAPSRST
                     ADPSYQRPLVNRRERSS"
     gene            5825..6721
                     /locus_tag="VFC01_13935"
     CDS             5825..6721
                     /locus_tag="VFC01_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN80766.1"
                     /translation="MPGEKYEYGIDFDRIAAIDFHTHVEVDGHGHKAYDDELVDAVGK
                     YFKMAPGALSSVDALADEYRRHNTAAVVFTIDARTAMRHVPNSIEDLVAGAIRNNDVL
                     IPFGSVDPWHERRAVHRVHELARDYGVKGFKFHPSMQAFEPNDRRFYPIYEAIAEANV
                     PALFHTGQTGIGAGLPGGHRIKLRYSDPMLLDDVAADFPELIIVMAHPAVPWVDSQIS
                     IATHKANVYIDLSGWSPKYFPPQLVHAVSRQLRTKVLFGTDYPYIKQDRWRRDFEGLE
                     IAPDIARLILKENALRVLGVTK"
     gene            6792..7574
                     /locus_tag="VFC01_13940"
     CDS             6792..7574
                     /locus_tag="VFC01_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN80767.1"
                     /translation="MSVRQGAPLGAPIWIDLLTSDLDRAQQFYGAVFGWTFESAGPEY
                     GGYVNAFKDGKPVAGLMGNTPEMRSPDGWTTYLHTGDITATVAAATAAGGVSCVEPME
                     IKDKGWMGLLTDPTGAFVGLWQPTGHEGFAVVNEAGAPVYFQLTTRDYGKALDFYREV
                     FGWQTDTVSDTDEFRYSTAIFDGQASPASGRYPQLGVMAEDVAPNWFFFLGADDVDKT
                     VQLVKDNGGSVIRDAEDTPYGRLAAVADPTGAGFNLSSLDQY"
     gene            complement(7546..9264)
                     /locus_tag="VFC01_13945"
     CDS             complement(7546..9264)
                     /locus_tag="VFC01_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZN80768.1"
                     /translation="MSGHIIVCGDDALGMRIVEELNNAGTRVVTLQAPEGLAAAGVAN
                     ASAVICADDDDDLNLEIALLARQANPNVRVVARLANTVLRLAMAEGNGPGAILDIADL
                     AAPAVVESCLSRTTHTMSIAGIDFVVSGAEAPRDATLREMYGDLAPVAVVSGENSPAP
                     GEVVACPGRDLPVHQGDWTAMIGAADELVAQGIKVPKPLAHRRVRRPPIRRLTDALRA
                     FREDINPMFYRALAVSASLLIGSTIMLRFAYQFPGMDWVDALYFSTETIATVGYGDFN
                     FLDQPTWLRLWGVVMMFSGVFTTAIVVAFVADVLLSRRLAQSATRQKVRYLRNHVVVV
                     GLGSFGIRVASDLKSAGYDVAVIERDDDNRFLSSAAELDIPVIVGDATLRQTLEAARV
                     HEARAVAVLTQDDMVNIETGIVIREMLSPRVMPEVVRPRVPIVLRVYDRTLGAAVGQR
                     FGFAYVHSTVDLAIPWFIGAAMGLQVLGTFSVRHRSFVVGGMRIEAGSELDGMRMFDL
                     STQTRVIAITRSDAPVQLHPRRDTRLSGGDTAYLVGPYRELLDTLRKGQGAQQTDVST
                     DPATTG"
     gene            9316..10044
                     /locus_tag="VFC01_13950"
     CDS             9316..10044
                     /locus_tag="VFC01_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HZN80769.1"
                     /translation="MPTSPKFYVKACINGARTPDAHPNLPVTPEQLAAEALAAHGAGA
                     KAVHMHPKTADGVDSLLPEHVDAAVDAVRHAVPGLPLGVTTGFWALPDADARLRAVDA
                     WSVLPDFASVNWHEPGSEDLARLLLSKGLGVEVGIFHTEAAESWAASEVAQHCMRVMI
                     ELQADGDVATADDLLSQVLAAGSPAPVLLHGLDESCWPLLEHAGVRGVQTRIGLEDTL
                     RLPDGSSAPDNAALVSAAVQLLSR"
     gene            complement(10034..10480)
                     /locus_tag="VFC01_13955"
     CDS             complement(10034..10480)
                     /locus_tag="VFC01_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN80770.1"
                     /translation="MSDLPEWARRLDLSPHPEGGWFKETWRSGLTMPQSALPPDYTGP
                     RSAGTAILFLLMPGQQSAWHTVRSAELWLYHFGGPLLLQMGAEQDTATTHLLGADIIA
                     GEQPQLVVPPGHWQRARPRDDVPCLVSCVVVPGFDYADFALAAPTD"
     gene            10526..11329
                     /locus_tag="VFC01_13960"
     CDS             10526..11329
                     /locus_tag="VFC01_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN80771.1"
                     /translation="MATGKAPIVLIHGLWMPPHSWRGWIERYSAAGHTVHAPAWPGVS
                     KLEEELDHAKAPADIGVVDVVDHYDAVVRALPEPPILMGHSFGGLITQMLLSRGLGRA
                     GVAIHPAAPRGVYRLPLSVLRATFPVFRRPSNRHRAVPLTSAEFHYAFANTVSRAESD
                     SWHAKLAIPAPGRPLFQSAIANFTPKSRAATVVDFTKPDRAPLLLIAGGRDHIVPSSV
                     VYENFNRYRRSPAATDFKLFDDRSHLTAALDGWSDVADYALTWTAGYSG"
     gene            11339..11773
                     /locus_tag="VFC01_13965"
     CDS             11339..11773
                     /locus_tag="VFC01_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80772.1"
                     /translation="MARLRPGWLVVLCAAVLAVSVWLPWLTSQADGGGRANAIGGIVG
                     TMPVPPDGFGVGQLIVLLASTLIVAGAMAARELFGRLASTAALTISVLLVVLTMWYYR
                     LYVYSPVAAGYGLYLGAAAAVAAVMLSVWAMVAAWSAAGRMS"
     gene            11770..12366
                     /locus_tag="VFC01_13970"
     CDS             11770..12366
                     /locus_tag="VFC01_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN80773.1"
                     /translation="MSGFVDPVSLTGERWVTLEPLAREHLPEIAAAAADGELGRLWFT
                     HAPKAAAAEQWVDMRLAAQNPDEGLTFVVRSLDGAFIGSSSYLNVDAPNRRLEIGNTW
                     YVASARRSGVNSETKLLMLGHAFDQLGCVAVEFRTHFFNSTSRAAIERLGAKLDGILR
                     SHQVLADGSRRDTVVYSILDIEWPAVRNNLRHRLDSNG"
     gene            complement(12363..13298)
                     /locus_tag="VFC01_13975"
     CDS             complement(12363..13298)
                     /locus_tag="VFC01_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019932446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN80774.1"
                     /translation="MNSRRLGLFTRLLPDRPDAPASLLYSQAREQFLLAERLGYDVGW
                     VAQHHFDVEEGGLPSPLVFLAWMAAQTERIRLGTAIITLALEDPIRVAEDAAVLDALC
                     EGRLELGVGTGGDRNGLFANNFQRLRDALHGAGLDARHRPMHPSGHRLLDDLWQATFS
                     VEGGSRAARDGCGLLLSRTQPRSVDTALHDIQQPIVDAYRENLPDGRPPRIGASRSVF
                     VLRDGVRAKELARQGVAGHVDWLRRNDLPVPDVIDVNAGTPAEVIDMLAADSTILAEA
                     TDLIVQVHPVDPPGEYVLESIELIATEAAPALGWR"
     gene            complement(13314..13763)
                     /locus_tag="VFC01_13980"
     CDS             complement(13314..13763)
                     /locus_tag="VFC01_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZN80775.1"
                     /translation="MLTFDPFSDLDAWTRGLLTSQTGSSRTPRFMPMDLCKIDDHYVL
                     TADLPGVDPGSVDVNVDNGTLTISAHRTARSEESVQWLASERFFGTYRRQLSLGEGID
                     ASAISATYENGVLTVTIPLAERAKPRKIDVAHGGGQKSIEPTTVESE"
     gene            complement(13860..14414)
                     /locus_tag="VFC01_13985"
     CDS             complement(13860..14414)
                     /locus_tag="VFC01_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HZN80776.1"
                     /translation="MTTLENRPNSALLVVDVQNCVVEGAHEREAVVANIDSLVDRARR
                     ENVPVVWVQHSDEDIIRGSDDWQIVPELTPADGEPRVDKNYGDSFEATTLENVLSGLE
                     VGRLVVAGAETDACVRSTVHGAFVRGYYVTLVSDAHTTGDKTQWGAPPPGQVIAHTNL
                     YWSYQSAPGRTAATVETKDVAFGN"
     gene            14479..14859
                     /locus_tag="VFC01_13990"
     CDS             14479..14859
                     /locus_tag="VFC01_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019664426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HZN80777.1"
                     /translation="MPAATQDLLAGVRRLCLALPEVTEGLTHGGETWFVRRRSFAKFV
                     DPDKHRLDERHVAFWAAAPPGARNELVAEDPQRFFVPRFGGYDWVGMRLDLQPAGPDW
                     QEVAEILTDAYRQVAPKFLIARLD"
     gene            complement(14840..15709)
                     /locus_tag="VFC01_13995"
     CDS             complement(14840..15709)
                     /locus_tag="VFC01_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HZN80778.1"
                     /translation="MLGLLGDEWTLLVIQQALLGATRFAEFKVRLPISNSVLTRRLQS
                     LTRDGLLDRRVYQLNPTRSEYLVTPRSRSLWPVLVSIWEWERRWVPDHAESLPRMRHI
                     ACGTDFAPLLVCRSCNEAASDKDVVAQWGPSGSWPRSIPVASTRRRSEADQRDAAGLF
                     PQTMSVLGNRWGFALLVAAFVGISRFTDFQTQLGAPPGSIADRLAIFTANGVIGSTDN
                     RYRLTEKGRALFPVLVSALQWAQHWFQAPEGPAVTLTHTVCGGRFGASLVCDQCTEPL
                     TGVQVAPVSRAGR"
     gene            complement(15751..17277)
                     /locus_tag="VFC01_14000"
     CDS             complement(15751..17277)
                     /locus_tag="VFC01_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HZN80779.1"
                     /translation="MAATNPEIVAKYLSTLPEDDDHPYRTGPWRPQTTEWDADDLTVV
                     EGEVPADLDGVYLRNTENPLHPALKFYHPFDGDGMVHVVGFRDGKAFYRNRFIRTEGF
                     LAENEAGGPLWPGLAEPVQIAKRDKGWGARTLMKDASSTDVIVHRGTALTSFYQCGDL
                     YRVDPYSGDTLGKAAWGGEFPFDWGVSAHPKVDDETSELLFFNYSKEAPYMHYGVVDE
                     NDQLVHYVDIPLPGPRLPHDMALTRNYVILNDFPLFWDPKLLQADVHLPGFHPDMPSR
                     FAVIPRRGGTDEIRWFEADPTFVLHFTNAYEDGDEIVLDGFFEGEPSPVDSLSGDKWD
                     KAFRFLALDRLQARLHRWRFNLVTGETHEEQLSDSITEFGMINGGYAGGKYRYTYAAT
                     GKPGWFLFDGLVKHDLHSGAEERFAFEDGVYGSETAMAPRVGSTDEDDGYLVTLTTDM
                     NADASYCLVFDAARVGDGPVCKLALPERISSGTHSTWASGSQLRRWHETDSAAAAIGL
                     "
     gene            complement(17279..18490)
                     /locus_tag="VFC01_14005"
     CDS             complement(17279..18490)
                     /locus_tag="VFC01_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HZN80780.1"
                     /translation="MGTPNVWILGGYQSDFARNFTREGHDFADLTAEVVDHTLTGARV
                     DAADIEVVHVANAFGELFTRQAHLGAMPASVNDGLWNTPASRHEAACASGSVATLAAL
                     SDLRSGAYRTALVVGVELEKTVSPDTAAQHLGAAAWTDHEGGEAKFMWPYLFDRVADE
                     YDRRYGIDDAHLRAIAQLNFANARANPNAQTRDWTVPDPIRADEANPPIEGRLRRFDC
                     SQMTDGGAGIVLVTDEYLRDHPDVRPIGRVDGWGHRTVGLGLQQKLDRAAGDPYVLPH
                     VRSAVLDAFGRARVTLDDVDGFEVHDCFTPSEYLAIDHIGLTGPGESWKAIENNEIEL
                     GGRLPINPSGGLIGGGHPVGASGVRMLVDAAKQVSGTAGDYQVDGAKRFGTLNFGGST
                     ATTVSFVVSGV"
     gene            18690..19376
                     /locus_tag="VFC01_14010"
     CDS             18690..19376
                     /locus_tag="VFC01_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HZN80781.1"
                     /translation="MTQPRNYDLARRMAELARASAAPRSVEDVLSDVTAAAKELIPGT
                     DTAGVLLIGKAGKFDSVAGTSDLPHLLDELQMKFNEGPCVEAALDELIVRTEDFRSEE
                     RWPKYSTAVVEIGVLSGLSFKLYTADRTAGALNLFSFKPNAFDAEDETTGVVLAAHAA
                     AAILSSRHGEQLESALTTRDRIGQAKGIIMERFGVDDVQAFEMLRRLSQDSNTRLIDI
                     AQRVIDTRGD"
     gene            19475..19891
                     /locus_tag="VFC01_14015"
     CDS             19475..19891
                     /locus_tag="VFC01_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80782.1"
                     /translation="MGGAVVAAAVACGSPSSEPADPMRTSEPVPSPAAASLQAAVALP
                     PNADGDPACPVADAWGKDPFGAGILVTHWSDDTAPVTVLVRTRVGNDQAQRALLGPGE
                     LRLFEFPEIDQAAVSEVIIMTNTRRCYVAADPAALG"
     gene            complement(19898..20137)
                     /locus_tag="VFC01_14020"
     CDS             complement(19898..20137)
                     /locus_tag="VFC01_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80783.1"
                     /translation="MSHNLKTVVARGVLSAGVALVGLGLASGTAHAFDPQPDPPSNPR
                     VKVNQQKSYADGSVRVGDGSVRLGDGSVTVATPGW"
     gene            20958..>21255
                     /locus_tag="VFC01_14025"
     CDS             20958..>21255
                     /locus_tag="VFC01_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80784.1"
                     /translation="MAATALAGPGCVASGVLVVTVSGTSARSGAVVVITVSATAPPFA
                     FCGWVDGETESAAVGARLGETVASWGTAIGSETTEADSGEAAARVVPAGAGLAVC"
BASE COUNT         3665 a         6967 c         7007 g         3616 t
ORIGIN      
        1 cccagcgata cccgcgacag actcctgtca gatgtcgcgt cgggcgcggt cagggcagcc
       61 gttgcgcttg aatcgacgga gttcgtcccc gatgccgagg gcgccgaccg cgtcctggtg
      121 atcgccgacg gcggcctcgc agtggcaagc ccggcggtca ctcctcaacc ggtcctcgac
      181 gaaacacgtc ggctggccac cgtcaccgca gacgacggtg cagagatgct ccagttcgaa
      241 ggcgaccacg ctgccgccgt tcgtcgcctg ctggaccggg ctgccgtggc cgttgcctgc
      301 gatagcttag ggctatgtga ggcaatgctt tccgcgaccg tcggctacgc gaaggtgcgc
      361 caccagttcg gcagacccat cggctccttc caagccgtca agcacgcctg cgccgacatg
      421 caggtgagta tcgccgtctc gcgccaactc gtcagcgccg cagtcgaagc cgtcgcagcg
      481 gagcgacccg atgccgccac ggcagccgcg atggccaagt catacgtctg tagcgccgcc
      541 gtcgacgtcg tcggtaaggc catgcaactg catggcggca tcggctacac gtgggagagc
      601 ggcatccacg tctacctcaa acgcgccgct ctcaaccgtt cgctgttcgg ttcaccggca
      661 gcacaccgca aacgactcgc tcaacgctat ctataaagga gtttcgacta atgggcgtac
      721 ccgtctacaa acgcattctc gacctgttcg aggccgaggg tgtcaacacg ctgttcggca
      781 ttcccgaccc gaacttcgtg cacatgttcg ccgaagccga cgcccgcggg tggtcggtgg
      841 tggcgccgca tcacgagttg agcgccggct tcatggccga ggccgtctcg cgcatgaccg
      901 gcgggccggg cctgtgcatc gggacactgg gaccgggtat ggccaacatc gccggagcga
      961 tccaatgcgc caaggtggag aactcgccgg tcatcttcct cggcggccag cgggcccgga
     1021 tcaccgagcg gcgggtgcgc cgcggtcgca tccaattcgt ccgccaggag ccactcttcg
     1081 cgccatcagt gaaatacagc gcgtcgatcg agtacgccga ccagaccgac gagatcatcc
     1141 gcgaggcgat ccggcgcgcg atgtccggca cgccaggacc gagctacgtc gagtacccgg
     1201 cgcacatcct gctcacagag ctcgatgtgc cggatccgtt gccgccgaat cggtaccggc
     1261 tggtcaacca gggggcgggc gcaccggaag ttgccgaggc cgcaaagctg attcgagagg
     1321 ccaagagccc gattctgttg gtgggacacg gtgttcacac gtcgcgcacc cagtctgcgg
     1381 tcaaggaact cgccgacctg atggcctgcc ccgtcatcca aacatcgggc ggcacctcgt
     1441 tcatcccggg gctcgaggac cgcaccttcg cctacggctt ttcgccgtcg gccgtcgacg
     1501 ccgtcgtcaa ctccgatctg tgcgtcgcgc tgggcaccga gctcggcgag cctgtgcact
     1561 acggcaagac ccgacattgg gcggagcgca acgacattcg caagtggatc tacgtcgagc
     1621 aggacccgat ggcgatcggc gtgaaccggc aaatcgacgt gccgctcgtg ggtgacctgc
     1681 gcggcgtcgt cccgcaactg gtcgaggcgc tcaaagacac accgcgacag ccgtcgccga
     1741 atctggacgt gctgatcaag aaggacgccg aggaattggc gcagctcgcc gagaacgcac
     1801 ccacgggcag aactccggtt catcccgcac gattcgtcgt cgaagccacc aaggcgttcc
     1861 cgaaggacgg catcctggtg cgcgacggcg gggccaccgt catcttccag tggacctact
     1921 cgcaagccaa gccgcgcgac gtgatctgga atcagaactt cggccacctc ggcacggggc
     1981 tgccgtatgc cgtcggtgcc tcggtggccg agggtcgcaa gcgtccggtc atgttgctca
     2041 ccagcgactc cgcgttcctg ttccacatcg ccgaattgga gacggctgcg cgggagggtc
     2101 taccgctggt gtgtgtggtc ggcgtcgacc atcagtgggg cctggaggtg ggcgtctaca
     2161 agcgcacgtt cgagcagccg tcgccgcagc ccggcgtgca ctggagcaag gacgtgcggt
     2221 tcgacaagat cgccgaaggt ttcggctgcc acggcgagta cgtcgaaaag gaggacgaga
     2281 tcggcccggc catcgagcgc gcctacgcca gcggcaagac cgccgtggtg cacgtgtgca
     2341 tcgacccgaa ggccaactcg gaggagatgc cgaaatacga cgaattcaga acctggtacg
     2401 ccgagggcac gcagtaagga agggtagagg aatgcgcgaa tatctcaagt tctatatcga
     2461 cggccagtgg gtggacccgg tgcggccgaa cactctcgac gtcgacaacc cgagaacgga
     2521 gcaggtctcc ggcaagatct cgatcggttc gtccgcagac gtcgacgtcg cggtgcaggc
     2581 ggcgcggcgg gcgttcgcct cgtggtctca gaccagtcgg gaggagcgcc tcgacgtgct
     2641 ccaagcgatg ctggccgaat accaaaagcg cgcaggcgat ctcgccgacg cggtaagcga
     2701 ggagatgggt gccccaccct cactggcggc gggccctcag gtcaacctcg gcatcggaca
     2761 tctggcgacg gccatcgacg tcctcaagaa cttcgagttc gaagagcagc atggagccac
     2821 gctggtggtc aaagagccga tcggtgtctg cggcctgatt acgccatgga actggccgat
     2881 caaccagatc gccgtcaagg tgtacccggc gctggcgact gggtgcacga tggtgctcaa
     2941 gccgtccgag gtggcgccgt actcggcgca gatcttcacc gaaatcgtcg atgccgcagg
     3001 cgttcccgcg ggagtataca acatggtgtt cggcgacggc cccggcgtcg gctcggcgat
     3061 ctcgagccat cccgacatcg acatggtgtc gttcaccggg tcgacacgtg cgggtgtgga
     3121 ggtcgcgcgc aacgccgcga cgacggtgaa gcgggtgagc caagagctcg gcggaaagag
     3181 ccccaacatc gtgctggacg acgaggactt cgccaagagt gtgaccgcag gcgtgtcggt
     3241 gatgatgatg aacagcggcc agagctgtaa cgccccgtcg cgcatgctgg tgccgaactc
     3301 gcgcatggac gaggccgtcg cggtggccca gggggtcgcc ggagcggtca ccatcggcga
     3361 cctgaacgac aaatcggcga tcgggccggt cgcgtccaag gcacagttcg acaagatcca
     3421 gggcctgatc cagaagggca tcgacgaggg tgccacggtt gcaatcggcg gaaccggtcg
     3481 gcccgacggc ctggacactg gctatttcgt caagcccacc gtcttcgcgc acgtgaccaa
     3541 tgacatgacg atcgctcgcg aggagatctt cgggcccgtg ctgtgcatcc tcggctacga
     3601 caacctcgat caggccctcg acatcgccaa cgacacggaa tacggcttgg caggctacgt
     3661 ttcggctgcc gatctcgaca aggcgcgctc ggtggcccgc cggatccgtg ccggctcggt
     3721 cgcgatcaat catgccttcg acatgaatgc gccgttcggc ggctacaagc gcagtggcaa
     3781 cgggcgcgag tgggggcagt tcgccttcga cgagtacctg gaaaccaagg ccgcactcgg
     3841 ctacgcgccg gaggcgacgg ccggctaggg cggaatggac gccagcacag cggaattgac
     3901 ggcgctgctc gaccgcgaca aggtccgcga ctgtatcgcg cgccttgctc gcggcgagga
     3961 ccgccgtgat gctggactca tcagtgggtc cttctggccg gacgcgacga tcgatctcgg
     4021 tgtgttcgcc ggatccttcg acgagtatct ggcgtgggtg gttcccggct caccgctgat
     4081 tccggtgact cagcatgtgt tgggacagag cgtgattgaa ctcgacggcg ctacagcctt
     4141 tgctgaaacg cacgtgaccg cctaccaccg gctgaccgca gacgatggcg accgcgacac
     4201 cgtcatcggt gggcgttacc tcgaccggtt ggacaagcgc ggcgctgaat ggcggatcgg
     4261 ccagcgcacc atgctctacg attggtatca cgacttcggc cgatcgaccg actgggccca
     4321 gggcctgatg gggttgcagt tcagtaccga tcactacacc ggcagcgccg accttgacta
     4381 cagcgaaacg ttcttcggca aaggcccgaa atgggagcgc tgacaggcaa agtcgcagtg
     4441 gtcaccggtg ccagccgtgg aatcggcaaa ggcgtcgccg tggcgctcgg ggcgcaggga
     4501 gtcacggtct atgtcacggg ccgtaccatg acgccgggtt cccatccgct gcccggcact
     4561 gtgggcgaca ccgcgaaaga ggtcacccgc cgcggcggtc aaggcgtggc cgtgcaggta
     4621 gaccacggtg acgacgcgca agtcgcggcg ctcttcgacc aaatcaagag cgaccacggc
     4681 cgactcgaca tcctggtcaa caacgcgttc gcacttcccg aggacctgac cgaacccggc
     4741 gagttctggg agaagccgct aagccattgg gacatggtcg atgtcggcgt ccgctcgaac
     4801 tatgtcgcgg cccggcacgc ggcaacaatg atggtcccgc aggggtccgg cctgatcgtc
     4861 gccatctcgg gctacgccgc ggtcacttac acctacggcg tcatcttcgg catgtgcaag
     4921 acggcggtcg accgcatggc ccgcgacatg gccgtcgagt tcgaaccgca caacgttgcc
     4981 tcgctttcgc tgtggcaagg ccttaccttc accgagcggg cccagcgcaa cttgcgtcgc
     5041 agcccggaga tgaccaagca gacagtcacc gaccccgcgg tcggatgctc gcccgagttt
     5101 cccggccggg tgatcgcggc gctggcgtcc gacccagatg tgatgaagcg gtcgggcggc
     5161 accttcatca ccgcggaggt cgcctgggac tacggaatca ccgacgtcga cgggaaagtc
     5221 gtcccctcac tgcgcgccga gcgtggcgaa cccgtctggc gtccgatcgc tgaggcgggc
     5281 catggacgct gaccgcatcg ccctgctcgt cgataggcag gacatcctcg actgcctcac
     5341 acggttcagc cgcggcatgg accgcttcga ccgcgacgtg ttcctctcgg catttcatcc
     5401 ggacgcggtc atcgcggccg gtgacttcgt cggcggtccg gcagatctct tccagtgggc
     5461 cacggccatg cacgagcgcg ggcagattgc gacgcaccac aacctgctca accacacctg
     5521 cgagatcgac ggcgacaccg cacacgccga gacctatttc ctgttcgccg cgcggaaccg
     5581 cgacgacacc aactggatcg cgggcggccg ctacctggac cggctgcacc ggcgcgacgg
     5641 agcgtggcgg atcgccgtgc ggaccaacgt catcgaatgg tcaggcatgg tcccgaccat
     5701 gccgttgccg ttcgccgaag tgcccgacat ccacggaaat ggggctccgt cgcgcagcac
     5761 ggccgatccg tcctatcagc ggcctttggt caaccgccgc gagaggtcgt cctagactgc
     5821 ggccatgccc ggcgaaaagt acgaatacgg aatcgatttc gaccgcatcg ccgcgatcga
     5881 cttccacact cacgtggagg tggacggtca cggccacaag gcctacgacg acgagctggt
     5941 cgacgccgtt ggaaagtact tcaagatggc gcccggtgcg ctgtcgagcg tggacgcgct
     6001 ggccgacgag taccggcgcc ataacaccgc cgcagtggtg ttcaccatcg acgcgcgcac
     6061 ggcaatgcgg cacgtgccga actcgatcga ggacctcgtc gcgggcgcta tccgcaacaa
     6121 cgatgtgctg attccgttcg gcagcgtgga cccatggcat gagcgacgcg cggtgcaccg
     6181 ggtccacgag ctggcgcgcg actacggcgt caagggtttt aagttccacc ccagcatgca
     6241 ggcgttcgaa ccgaacgacc gtcgcttcta ccccatctac gaggcgatcg ctgaagcgaa
     6301 tgtgcccgcg ctgttccaca ccggccagac gggaatcggc gccggactgc ccggcggcca
     6361 tcgcatcaaa ttgcggtact ccgatcccat gctgctcgac gacgtcgccg ctgacttccc
     6421 ggagctgatc atcgtgatgg cccaccccgc ggtgccgtgg gtggactcgc agatctcgat
     6481 cgccacccac aaggccaacg tctacatcga cctgtccggc tggtcgccga agtacttccc
     6541 gccgcaactg gtacacgccg taagtcgtca gctgcgcacg aaggtgctct tcggtaccga
     6601 ctacccctat atcaagcagg accggtggcg ccgcgacttc gaagggctcg agatcgcgcc
     6661 cgacatcgcc aggctgatcc tcaaggagaa cgcgcttcgg gtgctgggtg tcaccaagta
     6721 gcgcgatgag ttttcacagc ggccgtggtc gatatccgtg accgacccac accacctcga
     6781 aggagaccgt cgtgtccgtt cgccaaggcg caccgttggg tgccccgatc tggattgatc
     6841 tgctgacgtc cgacctggac cgcgcccagc agttctatgg cgccgtgttc ggctggacct
     6901 tcgagtccgc cggtcccgaa tacggcggtt acgtcaacgc gttcaaggac ggcaagcccg
     6961 tcgccggctt gatgggcaac acccccgaga tgcgctcgcc cgacggctgg accacgtatt
     7021 tgcacaccgg cgacatcacg gccaccgtgg ccgcggccac cgccgcgggc ggcgtgagtt
     7081 gtgtggaacc gatggagatc aaggacaaag gctggatggg cttgctgacc gatcccaccg
     7141 gggctttcgt ggggttgtgg cagccgaccg ggcatgaggg cttcgccgtg gtgaacgagg
     7201 ccggggcacc cgtgtatttc cagctcacca cccgcgacta cggcaaggcg ctcgacttct
     7261 accgcgaggt gttcggctgg cagaccgaca cggtgtctga taccgacgaa ttccggtaca
     7321 gcacagcgat tttcgacgga caagcgtccc ccgcaagcgg gaggtacccc cagctcggcg
     7381 tgatggccga agacgtcgcg ccgaactggt tcttcttctt aggcgccgac gacgtcgaca
     7441 agaccgtgca attggtcaag gacaacggcg gcagcgtgat ccgtgacgcc gaggacacgc
     7501 cgtacggccg gctggctgct gtggcggatc cgaccggcgc gggtttcaac ctgtcgtcgc
     7561 tggatcagta ctgacatcgg tctgctgggc accctggccc ttgcgcaggg tgtccagcag
     7621 ttcccggtag ggcccgacga ggtaggcggt gtccccgcca ctgagcctgg tgtcgcgacg
     7681 ggggtgcagt tgcaccggcg catcggaccg tgtgatcgcg atgacgcggg tctgggtgga
     7741 caggtcgaac atccgcatcc catccagctc gctgccggcc tcgattcgca tgccacccac
     7801 cacgaacgag cggtgccgca cagaaaacgt gccgaggacc tgcaggccca tggccgcccc
     7861 gatgaaccac ggtatcgcga ggtccaccgt ggaatggacg tacgcgaacc cgaaccgctg
     7921 gcccacggcg gcgcccaacg tgcggtcgta cacgcgcaac acgatcggca cgcgcggcct
     7981 gaccacctcc ggcatcaccc gcgggctcag catctcgcgg atcacgatgc cggtttcgat
     8041 attcaccatg tcgtcctgcg tgaggacggc gacggcccgg gcctcgtgga cgcgtgcggc
     8101 ctcaagggtc tgacgcagcg tcgcgtcacc gacgatcacc gggatgtcga gttcggccgc
     8161 tgaggacaag aaccggttgt cgtcgtcacg ttcgatcacc gctacgtcgt aaccggcgga
     8221 cttcaggtcg ctggcgacgc ggatcccgaa ggagcccagt ccgacgacga ccacgtggtt
     8281 gcgcaggtat cgcaccttct gccgggtggc cgactgtgcc agtcggcgcg acagcagcac
     8341 gtcggcgacg aacgccacca cgatcgcggt ggtgaagacc ccggagaaca tcatcacgac
     8401 cccccacagc cgcagccacg tcggctggtc gaggaagttg aagtcgccgt agccgaccgt
     8461 ggcgatggtt tcggtggaga agtacagcgc gtcgacccaa tccatcccgg ggaactgata
     8521 ggcgaaccgc agcatgatcg tggagccgat cagcaagctc gccgataccg ccagtgcccg
     8581 gtagaacatc gggttgatgt cctcgcggaa ggcgcgaagg gcgtcggtta atcggcgaat
     8641 cggcggtcgc cgcacgcgcc gatgtgccag cggttttggc accttgatgc cctgcgccac
     8701 cagctcgtcg gcggcgccga tcatcgccgt ccaatcaccc tggtgaaccg gcaggtcacg
     8761 gccgggacag gccaccacct cacccggtgc aggggagttc tcgccggata ccaccgccac
     8821 gggcgccaga tcgccgtaca tctcccgcaa tgtggcgtcg cgaggggcct ccgcaccgga
     8881 gacgacgaag tcgatgccgg cgatcgacat cgtgtgcgtc gtgcgtgaca ggcaggactc
     8941 gacgacggca ggagcggcca agtcagcgat gtcgaggatg gcgccggggc cattgccctc
     9001 cgccatcgcc aaccgcagca cggtgttcgc cagcctggcc accacccgga cgttcgggtt
     9061 ggcttgtctt gccagcagcg cgatttcgag gttcagatcg tcatcgtcgt cggcacatat
     9121 caccgcactc gcattggcga ccccggccgc ggccagacct tccggggcct gcaacgtcac
     9181 cacgcgcgtg ccggcgttgt tcagctcctc gacgatccgc attccgagcg catcgtcacc
     9241 gcacacgatg atgtgcccgc tcattcgcag aacgctactg cggaaaaggc cgccgcgaag
     9301 ggcgaagatg gtcagatgcc cacgagtccc aagttctacg tgaaggcctg tatcaacggc
     9361 gcccgcacgc cggacgcgca tccgaacctg cccgtcacgc ccgagcagct ggccgccgag
     9421 gccctcgccg cacacggcgc aggcgccaag gcggtgcaca tgcatcccaa gaccgccgac
     9481 ggcgtcgact cactgcttcc cgagcacgtg gacgccgcgg tggacgcggt gcggcacgcg
     9541 gtgccgggcc tgccacttgg cgtgacgacg gggttctggg ccttacccga cgcggatgcg
     9601 cggctgcgag cggtcgacgc ctggagcgtg ctgcccgact tcgcatcggt gaactggcac
     9661 gagcccggct cggaggatct cgcccggctg ctgttgagca aggggctcgg cgtcgaggtc
     9721 ggcatattcc acaccgaggc ggccgaatcc tgggcggcct ctgaagtcgc ccagcactgc
     9781 atgcgggtga tgatcgaact gcaggccgac ggtgatgtcg caaccgccga cgacctgctc
     9841 agccaggtac ttgccgcagg ctcaccggca ccggtcctgt tgcacggatt ggacgaaagt
     9901 tgttggccac tactggaaca cgccggggtg cgcggcgtgc agacccgcat cggcttggag
     9961 gacacgctgc ggctgcccga cggttcgtcc gctccggaca acgccgcgct ggtgtccgcg
    10021 gcggtgcagc tgctcagtcg gtaggcgcgg ccagcgcgaa atcggcgtag tcgaaacccg
    10081 gtaccaccac acagctgacc aagcagggca cgtcgtcgcg gggacgggcc cgttgccagt
    10141 gccccggcgg cacgactagc tgcggctgtt ctccggcgat gatatcggcg ccgagcaaat
    10201 gcgttgtggc agtgtcttgt tcggccccca tttgcagcag cagcggaccg ccgaagtgat
    10261 agagccacag ttcggcgctg cgcaccgtat gccatgccga ctgctgaccg ggcatgagca
    10321 ggaacaggat tgcggtgccg gcgctgcgcg gaccggtgta gtcgggtggt agcgcggact
    10381 gcggcattgt cagcccgctg cgccaggtct ccttgaacca gccgccctcg ggatgagggg
    10441 acaggtcgag ccgacgcgcc cactccggaa ggtcgctcat cgcaaatgcc ccctcaatcg
    10501 gttctgggac tccacaataa agacgatggc taccggcaag gcacctattg tgctgatcca
    10561 cggattgtgg atgccgccgc acagttggcg cggctggatc gagcgctaca gcgcggcggg
    10621 gcacacggtg catgcacccg catggccagg agtatcgaag ctcgaagagg aactcgacca
    10681 cgccaaggcg ccggccgaca tcggcgtcgt tgacgtggtg gatcactacg acgccgtcgt
    10741 gcgggcgctg cccgagccgc cgatcctgat ggggcattcc ttcggcggac tgatcaccca
    10801 gatgctgctc agccgcgggc tgggccgtgc cggtgtcgcc atccacccag ccgcgccgcg
    10861 cggcgtctac cggcttccgt tgtcggtact gagggccacg tttcccgtgt ttcgccgacc
    10921 gtcgaaccgt caccgtgcag tgcctctcac gtcggcggag ttccactacg cgttcgccaa
    10981 caccgtttcg cgggccgaat cggactcctg gcacgccaag ctggcgatcc ccgcgccggg
    11041 ccggccactg ttccagtcag cgatcgccaa cttcaccccg aagtcgaggg cggcgacggt
    11101 ggtcgacttc accaagccgg accgagcgcc gctgctattg atcgctggag gccgcgacca
    11161 tatcgtgccg tcctcggtgg tctacgagaa cttcaaccgc taccggcggt cgcctgccgc
    11221 gacggacttc aagctgttcg acgaccggtc ccatttgacg gctgcgttgg acggctggag
    11281 cgacgtggcc gactacgcgt tgacgtggac ggccgggtac agcggatagt ctgaccgcat
    11341 ggctcgcctg cgccccggtt ggctggtcgt gctgtgcgcg gcggtgctgg cggtcagtgt
    11401 ctggttgccg tggctgacca gtcaagcaga cggcggcggc cgcgcgaacg caatcggcgg
    11461 cattgtcggc accatgccgg tgccgccgga cgggttcggt gtcgggcaac tcatcgtcct
    11521 gctggcttcg acgttgattg tcgcaggcgc gatggctgcg cgtgagctgt tcggccggct
    11581 ggcatcgacg gcggcactga ccatttcggt gctgctggtg gtgttgacga tgtggtacta
    11641 ccggctctat gtgtactcgc cggtggcggc gggctatggg ctttaccttg gcgccgcggc
    11701 ggcggtggcg gcggtgatgc tgtcggtgtg ggcgatggtt gctgcgtggt ccgcggcggg
    11761 ccggatgtca tgagcggttt cgtcgacccg gtctcgttga ccggcgagcg ctgggtgacg
    11821 ctggaaccgc ttgcgcgcga gcatcttccg gagatcgccg cggccgccgc cgatggtgag
    11881 cttggccgcc tgtggtttac ccacgcgccg aaggcggccg ccgccgagca gtgggtcgac
    11941 atgcgactgg cggcccagaa cccggatgag ggcctgactt tcgtggtccg cagtctcgac
    12001 ggcgctttca tcgggtcatc gagctatctg aacgtcgacg ccccaaaccg tcggctggag
    12061 atcggcaaca cgtggtacgt ggcttcggcc cggcgaagcg gggtcaattc cgagacgaag
    12121 ctgttgatgc tcggccatgc cttcgatcag ttaggctgcg tcgcagtcga attccgcacg
    12181 cacttcttca actccaccag cagggcggcg atcgaacggt tgggcgccaa actggacggc
    12241 atcctgcgca gccaccaggt gctggccgac gggtcgcgcc gcgacacggt ggtgtactcg
    12301 atcctcgaca tcgagtggcc tgcggtgcgc aacaatctgc gacaccgact ggattccaac
    12361 ggctaacgcc agcccagcgc gggcgctgct tccgtggcga tgagctcgat cgattccagc
    12421 acgtattcgc cgggcgggtc gacgggatgt acctgaacga tcaggtcggt cgcttcggcc
    12481 aggatcgtcg agtcggcggc cagcatgtcg atgacttcgg ctggcgtccc ggcgttcaca
    12541 tcgatgacgt cgggtaccgg taggtcgttg cgtcgcagcc aatcgacgtg acccgcgacg
    12601 ccttggcggg caagctcttt cgcccgcaca ccatcacgaa gcacgaacac cgaccgcgac
    12661 gcgccgattc gcggcggtcg tccgtccggc aggttttccc ggtacgcatc gacgatcggt
    12721 tgttggatgt cgtgcagcgc ggtgtccacc gatcggggct gcgtccgaga cagaagcagc
    12781 ccacagccgt cgcgcgcggc gcggctgccg ccctcgacag agaacgtggc ctgccacaga
    12841 tcgtcgagca ggcggtgtcc gctgggatgc atcgggcggt ggcgggcatc caggcccgcg
    12901 ccgtgcaggg cgtcgcgcag ccgctggaag ttgttggcaa acagcccgtt tcgatcccca
    12961 ccggtgccca cgccgagttc cagccgtcct tcacacaacg cgtcgagcac ggcggcgtcc
    13021 tcggccaccc ggatcgggtc ctcgagcgca agtgtgatga tcgcggtgcc gagccggatc
    13081 cgttcggtct gcgcggccat ccacgccagg aagacgagcg gcgacggcag gccgccctct
    13141 tcgacgtcga agtgatgctg cgcgacccag ccgacgtcat agccgagtcg ttcggcaagc
    13201 agaaactgtt cgcgcgcttg cgaatacagc aacgatgcag gcgcgtcggg acgatcgggg
    13261 agtagacggg tgaacagccc aagtcgtcgg ctgttcaccc gtctaggcct ccgctattcc
    13321 gattcgacgg tagtcggctc gatcgacttc tgcccgccgc cgtgggcaac gtcgatcttg
    13381 cgcggcttgg cacgttcggc cagcgggatg gtcacggtca acacgccgtt ctcatacgtc
    13441 gcggaaatgg ccgacgcatc gatgccctcg ccgagcgaca gctgccgccg gtacgtgccg
    13501 aagaaccgct cgctggccag ccactgaact gactcctcgg aacgagccgt gcggtgtgcc
    13561 gagatggtca gtgtgccgtt gtcgacgttg acatcgaccg agccaggatc gacaccgggc
    13621 aggtcggccg tcagaacgta gtgatcgtcg atcttgcata gatccatcgg catgaatcgc
    13681 ggggttcgac ttgatccggt ctggctggtc agcaggcccc gggtccaagc atcaaggtca
    13741 ctgaacggat caaaggtaag cacagcaatc cacctcctac gtcacctcag agcccgccac
    13801 cctggcgggc agccaaatca ctgtgcacct ggcattttag cactctcact cggagagtgc
    13861 tagttcccga aggcgacgtc tttggtctcg accgtggcgg cggtccggcc cggcgccgac
    13921 tggtagctcc agtacaggtt ggtatgcgca atgacctggc ccggcggcgg tgcaccccac
    13981 tgcgtcttgt cacctgtcgt atgggcgtcg ctgacgaggg tcacgtagta ccccctgacg
    14041 aaggcgccgt gcaccgtcga gcgaacgcat gcgtcggtct ctgctcccgc taccacgagg
    14101 cgtccaacct cgagccctga gagcacgttc tcgagcgtgg tggcctcaaa cgagtcgccg
    14161 tagttcttgt ccacccgcgg ctcaccgtct gccggtgtca gctccggcac gatctgccag
    14221 tcgtcgctgc ccctgatgat gtcctcatcg gagtgctgga cccacaccac ggggacgttt
    14281 tcccgccggg cgcgatcgac aagactgtcg atgttcgcga cgactgcctc gcgctcatgc
    14341 gctccctcga cgacgcagtt ctgtacgtcg acgacgagca gtgcgctgtt gggccggttc
    14401 tcgagtgtcg tcaatgtttt cccctcacca tcgattccat ctccgtcgac cgtaacgcgg
    14461 ccgtgcgacg atgcctctat gccagccgcg acgcaggatc tgcttgccgg ggtacggcga
    14521 ttgtgcctgg cactgcccga ggtgaccgag gggctgaccc acggcggcga aacctggttc
    14581 gtgcgccgac ggtcgttcgc caagttcgtc gaccccgaca agcatcggct cgatgagcgg
    14641 catgtcgcgt tctgggccgc tgcgcccccc ggtgcccgca atgagcttgt cgcggaggac
    14701 ccacagcggt tcttcgtccc gcggttcggc ggctacgact gggtcggcat gcgactggat
    14761 ctgcagccag ccggacccga ctggcaagag gtcgccgaga tcctcacgga tgcctatcgt
    14821 caggtggcgc cgaagttcct catcgcccgg ctcgactaac cggggccacc tgtactccgg
    14881 tgagcggctc ggtgcactga tcgcagacca gtgaggcgcc gaaccgcccg ccgcagaccg
    14941 tgtgggtcaa ggtcacggca ggcccctcgg gcgcctggaa ccagtgctgc gcccattgca
    15001 gcgcggacac cagcaccggg aacaacgccc tgcccttttc ggtgagccgg tatcggttgt
    15061 ccgtcgatcc gattacgccg ttggcggtga agatcgccag ccggtcggcg atcgaacccg
    15121 gcggtgcgcc gagctgggtc tgaaagtcgg tgaaccggct gattcccacg aaagccgcga
    15181 ccaacagcgc gaaaccccag cggttgccca acacgctcat cgtctgcgga aacaggccgg
    15241 ccgcgtcgcg ctgatcggcc tcggaccggc gccgggtcga tgcgacgggg atggaccgcg
    15301 gccacgaacc gctcgggccc cactgtgcga caacgtcttt gtcgctggcg gcttcattgc
    15361 acgagcggca gacgagcaac ggagcgaagt cagtgccgca cgcgatatgc ctcatgcgtg
    15421 gcaagctctc ggcatggtcg ggcacccatc gtcgctccca ctcccagatc gacacgagca
    15481 cgggccataa cgaccggctg cgcggggtca ccagatactc ggagcgggtc gggttcagct
    15541 gatagacacg gcggtccagc aggccgtcgc gcgtcagcga ctgcaggcga cgggtgagca
    15601 cggaattcga gatcggcagc cgcactttga attcggcgaa ccgcgtcgcg ccgagcaggg
    15661 cctgctggat gaccagcagc gtccactcgt caccgagcag tccgagcatg cgggctacgg
    15721 cgttgggctc cggctgctcc actacagcca ctacagcccg atcgccgccg ccgcggagtc
    15781 ggtctcgtgc caccgccgca actgtgaacc ggaggcccag gtggaatgcg tcccactcga
    15841 gatccgctcc ggcagagcca gtttgcaaac cggcccgtcg ccgaccctgg cggcgtcgaa
    15901 caccagacag tacgaggcgt cggcgttcat gtccgtggtc agtgtgacca agtagccgtc
    15961 gtcctcatcc gtgctgccga cgcgcggcgc catcgccgtc tcgctgccgt acacgccgtc
    16021 ctcgaatgcg aatcgctcct cggctccact gtgcagatcg tgcttgacta gcccgtcgaa
    16081 caggaaccac cccggcttcc ccgtcgcggc gtaggtgtac cggtactttc cgccggcgta
    16141 gccaccgttg atcatgccga actcggtgat gctgtccgac agttgttcct cgtgcgtctc
    16201 gccggtgacg aggttgaacc tccagcgatg cagcctggcc tggagccggt ccaacgccag
    16261 gaaccgaaac gccttgtccc acttgtcacc gctgagcgag tcgaccggcg agggttcgcc
    16321 ctcgaagaac ccgtcgagca caatctcgtc gccgtcctca taggcgttgg tgaagtgcag
    16381 cacgaacgtc gggtcggcct cgaaccagcg aatttcgtcg gttccgccgc ggcgaggaat
    16441 gacggcaaac cgagacggca tgtcagggtg aaaccccggc aggtgcacgt cggcttgcag
    16501 cagcttggga tcccagaaca gcggaaagtc gttgagaatg acgtagtttc gggttaatgc
    16561 catatcgtgc ggcagccgcg gacctggcag cgggatgtcc acgtagtgca caagctgatc
    16621 gttttcatcg acgacgccgt agtgcatgta cggcgcttcc ttgctgtagt tgaagaacag
    16681 cagttcgctg gtctcgtcgt cgaccttggg atgagccgag acgccccagt cgaacgggaa
    16741 ctcgccgccc caggccgcct tgcccagcgt gtccccggag tacgggtcca ccctgtacaa
    16801 gtcgccgcac tggtagaaac tcgtcagcgc ggtgccgcgg tgtacgatca cgtcggtgct
    16861 cgacgcgtcc ttcatcaagg ttcttgcgcc ccagcccttg tcgcgcttgg cgatctgcac
    16921 aggctcggca agacccggcc acagcgggcc gcccgcctcg ttctcggcga ggaaaccctc
    16981 cgtgcggata aacctgttgc ggtagaacgc cttcccgtcc cggaacccga ctacatgcac
    17041 catgccgtcg ccgtcgaacg gatggtagaa cttcagcgcc ggatgcagcg gattctcggt
    17101 gttgcgcaga tacacgccat cgagatcagc cggaacctca ccttcgacga cggtcaggtc
    17161 gtcggcgtcc cattcggtgg tctgcggacg ccacggcccg gtgcggtagg ggtggtcgtc
    17221 gtcctcgggc agtgtggaga gatatttggc gacaatctct gggtttgttg cggccatttc
    17281 agactccgct cacgacgaag ctcactgtgg tggctgtgct gccgccgaag ttgagcgtgc
    17341 cgaacctctt tgcaccgtcg acttgatagt cgccggccgt tccgctgacc tgctttgcgg
    17401 cgtcgacgag catgcgcacg ccggacgcgc cgaccggatg accaccgccg atcagtccgc
    17461 cgctggggtt gatcggcagc cggccgccga gttcaatctc gttgttttcg atcgccttcc
    17521 acgattcgcc cggtccggtc aggccgatgt ggtcaatggc gaggtactcg ctgggggtga
    17581 agcagtcgtg cacctcgaac ccgtcgacgt catcgagcgt gacgcgggcg cggccgaacg
    17641 cgtcgagcac cgccgaccgc acgtgtggca gcacataggg gtcgccggcg gcgcggtcga
    17701 gtttctgttg caaacccaga cccaccgtgc ggtggcccca gccgtcgacg cggccgatcg
    17761 gccgcacgtc cggatggtcg cgcagatact cgtcggtgac cagcacgatg cctgcgccgc
    17821 cgtcggtcat ctggctgcag tcgaaccggc gcagccggcc ctcgatcggc gggttggctt
    17881 catcggcgcg aatcggatcc gggaccgtcc aatctcgggt ctgcgcgttc ggattcgcgc
    17941 gggcgttggc gaagttcaac tgggcgatcg cccgcaaatg cgcgtcgtcg atgccgtatc
    18001 ggcgatcgta ttcgtcggcc acgcggtcga acaggtacgg ccacatgaac tttgcctcgc
    18061 caccctcgtg gtcggtccag gcggcagcgc cgaggtgctg cgctgcggtg tcaggagaca
    18121 ccgtcttctc cagttcgacg ccgacgacca gggcggtgcg gtacgcgcct gaccgcagat
    18181 ccgacaatgc cgcgagggtc gcgacgcttc cggatgcgca agcggcttca tggcgggacg
    18241 cgggggtgtt ccagagaccg tcgttgacac tggccggcat cgcgccgagg tgggcctgcc
    18301 gggtgaacaa ctcgccgaag gcattggcca cgtgaaccac ttcgatgtcc gcagcgtcga
    18361 ccctggctcc ggtcagcgtg tggtcgacca cttcggcggt caggtcggcg aaatcatggc
    18421 cttcgcgggt gaagttgcgt gcgaaatcgc tctgatagcc gccgaggatc cagacgttgg
    18481 gagtgcccat gacgcgacgg tactacaact aagagagtga ctcggccgat tcgcccatcc
    18541 ccggaggcgg ccccgccggc tctctgtctg aaccgacgag acctcagcga acgcgatagt
    18601 cgaccatcgc gcgcgcctgt actggggcat tgcccgctgt gtcgaatgac aaacgcatat
    18661 cccggcgtga catcgggaag ccttaaggga tgacgcagcc gcggaactac gacctggctc
    18721 ggcgaatggc cgagctggcg cgcgcttccg cggcgccgcg aagcgtcgaa gacgtgctgt
    18781 cggacgtgac tgcagcggcg aaggagctga tccccgggac ggatacggca ggagtgctgc
    18841 tcatcggaaa ggccggaaaa ttcgactccg tcgccggcac ctctgacctg ccgcatctac
    18901 tggatgagct gcagatgaag ttcaacgagg ggccgtgtgt ggaggcggcg ctcgatgaac
    18961 tgatcgtgcg gaccgaggat tttcgatccg aggagcgctg gcccaaatac tcaacagcgg
    19021 tggtcgagat cggcgtcctg agcgggttgt cgttcaagct ctacaccgcc gatcggaccg
    19081 caggggcgct gaacctgttc tcgttcaagc ccaatgcatt tgacgcagag gacgaaacca
    19141 ccggcgtagt ccttgcggcg cacgcggcgg cggcgatcct gtccagccgc catggtgaac
    19201 agctcgagtc ggcactgacc acaagggacc gcatcgggca ggccaagggc atcatcatgg
    19261 aacggtttgg cgtcgacgat gtgcaggcgt tcgagatgct gcgccggctg tcccaggaca
    19321 gcaacacccg gctgatcgac atcgcgcaac gggtcatcga tacgcgcggc gattaagcat
    19381 cgggacgcgg attgacgcgc gcgtaagcgg gtatttcact cgtattgggg acggatgcga
    19441 gggggacctt gtgcacgccg gagtatgcgc gatcgtgggc ggcgccgtgg ttgccgcggc
    19501 ggtcgcgtgc ggttcaccct ccagtgagcc ggccgatccg atgcgcacgt ccgagccagt
    19561 gccttccccg gctgccgcct cgcttcaggc agcggtcgca cttccgccga acgccgatgg
    19621 cgacccggcg tgtcccgtcg ccgacgcgtg ggggaaggac ccgttcggcg ctggcatcct
    19681 ggtcacccac tggagcgatg acaccgcgcc cgtcaccgtg ctggtgcgca cgagggtcgg
    19741 caacgatcaa gcgcagcgtg cgctgctcgg ccccggcgaa ctgcggttgt tcgagttccc
    19801 tgagatcgac caggccgccg tctccgaagt gatcatcatg accaataccc gcaggtgtta
    19861 cgtggcggca gacccggcag cgctgggctg aggccggtta ccagcctggg gtcgcgactg
    19921 tgacgcttcc gtcgccgagc cggacgctgc cgtcgccaac gcgcacactg ccatctgcat
    19981 atgacttctg ctggttcacc ttgacgcgag gattgctcgg cgggtcgggc tgtggatcaa
    20041 aggcgtgcgc ggtgcccgag gccagtccca gtccgaccag cgcaacgccg gcggacagga
    20101 cacccctcgc gaccacggtc ttcaggttgt ggctcatggt tctatccctt caatgctggc
    20161 ggctctgtgg tggccgcgcc gcaagtgaag cgggcgtgcg ctgctaccga tcccaagaac
    20221 tccggtgtga tcgcgaccga aaccatcgct gacctgcgaa aaacagtgag tgcccccgcc
    20281 aggattcgaa cctgcgacac cggctttagc agcagcggtt tgccgtcgcg ttctgttaat
    20341 tctcagaaac cactgctgag cagccggtcc cgagtgtgtt gcgtctcaac gcttctcgcg
    20401 gaatgtcgct gatgctcggc catatacgat gaataagcga tgagtgggaa ccgggcacgg
    20461 cgggggcagt gacagggata gatcggcgac ggtccagcgc atcgggaacc cttcgtctga
    20521 ctaccgcgtc ctaccgcata cggcccgcca tgtcggctcc ggtggcgggc ctcgttcatg
    20581 ccaatcaatg tgcgagtgaa ccgcccttct ccgccgacaa agggatacgg tgccgagtcg
    20641 gtgtaggcga atcaggcgtc tatttggcga ccatcggcag gacgctgcca gagttgggta
    20701 accaggcggc ccagataagt gcgattagta cgcgcgtgcc ggggcccgct gagcgtcgct
    20761 cgtcagagcc catgcaaccc tatccgttgc tctccgtagc gtccgtacct tttcttgtag
    20821 acgtagcagt acgtttcgca gacgtagcct gatctccact cgtggtttgg ttcccacccg
    20881 ccttgccggt caccaccttg tcgccgcgag tgctggacgc gtcggtcgtg taagtggtcc
    20941 gcgcggtggt ggactgcgtg gccgcaacgg cattggccgg ccccggctgc gtggcgagcg
    21001 gcgtgctcgt tgtcaccgtg tctggcacgt ccgcgcggtc aggagctgtc gtcgtgatta
    21061 cggtgtcggc gaccgcgccg ccgtttgcgt tctgcggttg ggtagatggg gaaacagagt
    21121 cagctgctgt tggggccagg ctcggtgaaa ccgtggcatc ctggggcacc gccatcgggt
    21181 cggagacaac cgaagcagac agcggcgaag cggcggcgcg agtggtccca gccggcgctg
    21241 gcttagccgt ctgca
//
LOCUS       DASRJA010000321         6060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_951447,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000321 DASRJA010000000
VERSION     DASRJA010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951447"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFC01_14030"
     CDS             <1..685
                     /locus_tag="VFC01_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HZN80785.1"
                     /translation="TSLIFDDVTGVPRRCLVLIPRVMQPNSDRVDAVRYRFVGPCIDP
                     RRENADDWTPPAGDGPLALMAFGTAYTDRIDLYRNVIGALDGTGWRLVIATGRVAPDE
                     LGDVHGWVQVRKTVPQPAVLRVADAFVTHAGMGSCTEGLWYGVPMLAIPQAVDQPSNA
                     DQLEAIGAGRHLTGVSPSPGQIRDAFLEVAADSQIRLRLKAIRDELHRVGGPGHAADA
                     VEDVASGRW"
     gene            744..1157
                     /locus_tag="VFC01_14035"
     CDS             744..1157
                     /locus_tag="VFC01_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZN80786.1"
                     /translation="MRIRIPAAAMAITMGLSVLASAPSSAASDEDQVRAVLNGMNASY
                     NRTDFGTFASHLCEAMLNAAGLEAGWYASRKSDGPTQITINSVDVTGGPRPQVAVANV
                     RFVAANHSDAKTLDVEFLREAGEWKACRYDAGQSV"
     gene            complement(1164..1679)
                     /locus_tag="VFC01_14040"
     CDS             complement(1164..1679)
                     /locus_tag="VFC01_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN80787.1"
                     /translation="MKETEHMSRRAQSKARMFAIVLALAAVPAAVAVVSPAIASADSC
                     SDAATNAPLPLEVGACADVLAQEARWLKAITAGDVPTVESILSPTYKHINSDGRLLSR
                     ADEIASTEPLPITMNPSEQLVDIAGDTAVIHGVNTMTQDGKVAGVSRFTDVFVLQNGV
                     WLALSAQETAT"
     gene            1728..2396
                     /locus_tag="VFC01_14045"
     CDS             1728..2396
                     /locus_tag="VFC01_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HZN80788.1"
                     /translation="MSGDWGSVLTELIPLALVVALSPLSIIPAVLVLHTPRPRPAGLA
                     FLAGWFVGLAALTAIFVEVSDLLGDLDKPPRWASWVRVLVGAALIVFGIYRWLMRKRS
                     AHTPGWMQSIGKLTPARAGLAGVALTVVNPKVLFICAAAGLAIGTAGLGTTRAWVAVA
                     WYVAVAGSTVAIPILAYTVSGDRLDESLRRLKDWMERQHAVLVAAILIVIGLLVLYKG
                     IHGL"
     gene            complement(2391..3023)
                     /locus_tag="VFC01_14050"
     CDS             complement(2391..3023)
                     /locus_tag="VFC01_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN80789.1"
                     /translation="MPSPATTTKRRGRRQGEPVSRDAVLSAAKRRFGSEGYEKTTLRA
                     IAEDARVDASMVLYLFGSKEELFRESLRLIIDPELLVAALTGDEDPDVGTRMVRTYLR
                     IWETPDTAASMRAMLQSATSNSHAHDAFRSFMQNYVLSAVSGVLGGGEQARLRALLAA
                     SQLVGTAMLRYIIEVPPLATLPVDDVVKLIAPTVTRYLTADAAELGLPAS"
     gene            3124..4254
                     /locus_tag="VFC01_14055"
     CDS             3124..4254
                     /locus_tag="VFC01_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN80790.1"
                     /translation="MTTQSPPRSTHHAEPTHEPPAALRVTGILIALAAALALIAIAFA
                     LPAAKSKPHDVPIGTVGPQAVTGQIADVLQRTAPGAFAVTIYPNEAELRTAIRDREVY
                     GGLSFGQQGPTLLTATGASPAVAQLLSQVGDGISQQAGAPLHTEDLAPPPAGDQRGTG
                     LAAAALPVTLAGLLPVIVLTMMIPLGVWARFAAIAAFSGLAGITIAVVLRYVLGSIDQ
                     NFWAVAGGLALGALAVAVFVLGLASLFGRVGLVLGALLALLLGNPLSGMTSAPEMLPA
                     GWGGLGQFLPQGANATLLRSTAFFDGAGASTPIAVLVCWAVVGALIVVIAAQRLQVEK
                     FRVSAESDAPGHPVPRDVPQPDLAAATSFRPRWTSDAHPAAG"
     gene            4321..5286
                     /locus_tag="VFC01_14060"
     CDS             4321..5286
                     /locus_tag="VFC01_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN80791.1"
                     /translation="MHEPPAAIRATAIVVALTTALAILAIAFALPAARSKPHDIPIGA
                     AGPQAASGQVADILEQHAPGAFALTYYPGEAALRDAIRNRDVYGGMSFRPDGRSLLIA
                     TGGSPVVAQMLTQIGNGVAQQAGVPLHTEDLAPPTADDPRGAGLAASALPITLAGLLP
                     AVALVLLVRREVWTRLVAAVVFAAGAGVTIAALLRYVFGSIDQNLWGVAAGLTLGLLA
                     AGLSVLGLGSLFGRGGLAVGALLALLLGNPLSGLTSAPEMLPSGWGTLGQWLPQGATA
                     TLLRSTAFFDGAGAATAIAALTCWAVTGTALVVIAAVRQGRSVPA"
     gene            5298..>6060
                     /locus_tag="VFC01_14065"
     CDS             5298..>6060
                     /locus_tag="VFC01_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN80792.1"
                     /translation="MGLDDRDSLRILRDAIDPALGSDELIRDFYTRWFAVDITARDLF
                     PPDMDVQRDAFAKAVTWLLSELIAQRADEPVAFLAQLGRDHRKYAVTQQHYDTMQDAL
                     LTTLRSHLIARWNDRLAAAAQDAVTLIIGVMRGSADAEKGPPYRDGTVIEHVRSTRDV
                     SVIRLQLDKPLYYHPGQYVTVQVPQWPRRWRYLSPAIPADRGGAIEFHIRSVTGGMVS
                     TAIVGETKPGDRWRLSNPHGGMHVDRDGGDVLMVAG"
BASE COUNT          906 a         2071 c         2031 g         1052 t
ORIGIN      
        1 cacctcgctg atcttcgacg acgtaacggg cgtgccccgc cgctgtctcg tgctgattcc
       61 ccgtgtcatg cagcccaact ccgatcgcgt cgacgctgtc cgctaccggt tcgtcggacc
      121 gtgcatcgat ccgcggcgag agaacgccga cgattggacg ccacccgccg gggacggtcc
      181 tctcgccctg atggcgttcg gcaccgccta caccgaccga atcgacctgt accgcaatgt
      241 gattggggcg ctcgatggca ccggttggcg cctcgtcatt gccacgggcc gggtggcacc
      301 cgacgagctg ggcgacgtgc atggctgggt gcaggtgcga aagaccgtgc cgcagccggc
      361 ggtgctccgg gtggccgacg cgttcgtgac gcacgcgggt atgggctcgt gcaccgaggg
      421 actctggtac ggcgtgccga tgctcgccat tccccaggcg gtcgatcagc cgtcgaatgc
      481 cgatcagctc gaggcgatcg gggcgggtcg gcaccttacc ggcgtttccc cgagtcccgg
      541 ccaaatccgc gacgcgttcc tggaggtggc ggccgactca cagattcggt tgcggctgaa
      601 ggcgattcga gatgagctgc accgcgtcgg cggtcccggg cacgcggccg acgcggtcga
      661 agacgtcgcc agcgggcgct ggtgatcgcg acttgtcggc gcgtcgaccc gtcatgcgcg
      721 caatcatcgg tcaggctgaa cgaatgcgca tcagaatccc ggccgccgca atggcgatca
      781 ccatggggct gagcgtgctt gccagcgcac catcgtcggc ggcctctgac gaagaccagg
      841 ttcgcgccgt gctcaatggg atgaacgcct cctacaaccg caccgatttc ggcacgttcg
      901 cgtcgcatct gtgtgaagcc atgctgaacg ccgctgggtt ggaagcgggt tggtatgcga
      961 gcaggaagtc cgacggaccc acgcagatca ccatcaactc ggtcgacgtg accggcggcc
     1021 cccgcccgca agttgccgtt gccaacgtcc gattcgtcgc ggccaaccac agcgacgcga
     1081 aaaccctcga cgtcgaattt cttcgcgagg ccggcgagtg gaaagcctgc cgctacgacg
     1141 ccggccagtc ggtgtagctc cgtctaggtc gccgtctcct gcgccgacag cgccaaccac
     1201 actccgttct gcagcacgaa cacatcggtg aatcgcgaga ctccggccac tttgccgtcc
     1261 tgcgtcatgg tgttgacccc atggatcacc gcagtgtcgc ccgcgatgtc gacgagctgc
     1321 tccgacgggt tcatcgtgat cggcaacggc tcggtgctcg cgatctcgtc ggcacggctc
     1381 agcagccgcc cgtcggagtt gatgtgcttg taggtcgggc tgaggatcga ctcgacagtc
     1441 ggcacgtccc cggcggtgat cgccttcagc cagcgtgcct cctgggcgag cacgtcggcg
     1501 cacgctccca cctcgagtgg cagcggagcg ttcgtcgcgg catccgagca actgtcggcg
     1561 cttgcgattg ccggcgagac gaccgcgacg gcggcgggaa ccgccgcaag tgccaacacg
     1621 atcgcgaaca tcctggcttt cgattgcgct cgtcggctca tatgttcggt ctccttcatc
     1681 gtcggttgct acgtttcatg tcgagcggtc cgacgaaagg gtaggcgttg tccggcgatt
     1741 ggggatcggt acttacagaa ctcattccgc tggcgctggt ggtcgcgctc tcgccgctgt
     1801 cgatcattcc ggcggtgctc gtgctgcaca ccccgcgacc tcgaccagcg gggctggctt
     1861 tcctggccgg ctggtttgtc ggccttgcgg cgttgacggc gatcttcgtc gaggtgtcgg
     1921 acctactcgg cgatctcgac aagccaccgc gttgggcgtc gtgggtgcgc gtcctcgtgg
     1981 gcgcggcgtt gatcgtgttc ggcatctacc ggtggctgat gcgcaagcgc tcggcgcata
     2041 cacccggctg gatgcagagc atcggcaagc tcacgccggc tagggccggc ctcgccggtg
     2101 tggcgttgac cgtcgtcaat ccgaaggtgc tattcatctg cgccgcagcg ggtttggcga
     2161 tcggcaccgc cggactggga acaacgcgcg cgtgggtcgc ggtggcctgg tacgtggcgg
     2221 tcgccgggtc aacagtggcg attcccatcc tcgcgtacac ggtgtccggt gaccgcctcg
     2281 acgaatcatt gcgacggctc aaggactgga tggagcgcca gcacgccgtt ttggtggccg
     2341 ccatcctgat cgtcatcggt cttctggtgc tgtacaaggg aattcacggg ctatgaggcg
     2401 ggcagtccaa gctccgccgc atcggccgtc aggtagcgcg tcactgtcgg tgcgatcagc
     2461 ttgactacgt catccaccgg cagcgtcgcc agcggtggca cctcaatgat gtagcgcagc
     2521 atggccgttc caaccagctg gctggcagcc agcagcgcgc gcagccgagc ctgctcgcca
     2581 ccccccaaca cccctgacac cgcgctcagc acgtagttct gcatgaagct gcgaaacgcg
     2641 tcgtgcgcgt gggaattcga cgtggccgac tgcagcatcg ctcgcatgct cgcggcggtg
     2701 tcgggcgtct cccagatccg cagatacgtg cgcaccatcc gggtgccgac gtcgggatcc
     2761 tcatcgccgg tgagtgccgc gaccaacagc tcgggatcga tgatcagccg cagcgactcg
     2821 cggaacagct cctctttcga cccgaacagg tagagcacca tcgacgcgtc gacgcgcgcg
     2881 tcctcggcga tcgcgcgaag cgtcgtcttc tcgtagccct cgctcccgaa tcgccgcttg
     2941 gccgccgaca gcaccgcgtc gcgggacacc ggttcgccct ggcgtcgacc ccggcgcttc
     3001 gtcgtcgtag ctggtgaggg catatgccga cgataacatt tcaatagttg ttgaaatagc
     3061 tgcctgtcgg cgttacgctc gggcggacag gaattcaacg cgagatgaaa tgaggagccg
     3121 gccgtgacca cccagtcacc accccgatcc acacaccacg ctgaaccgac gcacgagcca
     3181 cctgcggcgc tgcgagtcac cggcatcttg atcgcgctcg ctgcggcgtt ggccctgatt
     3241 gcgatcgcgt tcgcactgcc agccgcgaag tccaagccgc acgatgtgcc gatcggcacc
     3301 gtcggcccac aggcagtcac tggtcagatc gccgatgttc ttcagcggac cgcacccggc
     3361 gccttcgccg tcaccatcta cccgaatgag gccgaactgc gcaccgcgat tcgcgatcgc
     3421 gaggtctacg gcggcctttc gttcggacag caaggcccga cactgttgac ggccacgggc
     3481 gccagtccgg ccgtcgcgca gctgctgagc caggtcggcg acggtatttc ccagcaggcg
     3541 ggcgcaccgc tgcacaccga agacctggcg cccccgcccg ccggggacca gcgcggaacc
     3601 gggctcgccg ccgccgcatt gccggtcaca ttggccggac tgctgcccgt catcgtgctg
     3661 acgatgatga ttccgctcgg cgtgtgggcg cggttcgcgg ccatcgctgc attctcgggt
     3721 ctggcgggca tcacgatcgc ggtcgtgctc cgatatgtcc tcgggtcgat cgaccagaac
     3781 ttctgggccg tcgcaggggg gcttgcgctg ggagcgttgg ccgttgccgt gttcgtcttg
     3841 ggcctcgcgt cgctcttcgg acgggtcggc ctggtgctcg gggctctgct ggcgctgttg
     3901 ctcggaaacc cactatcggg gatgaccagc gcaccggaga tgctgcccgc agggtggggt
     3961 ggcctcggcc agttcctgcc ccagggcgcc aacgcgacgc tgctgcgctc gaccgcattc
     4021 ttcgacggcg ccggcgcgag cactccgatc gccgtcctcg tctgctgggc ggtggtcggg
     4081 gctctgatcg tcgtcatcgc cgcgcagcgc ctgcaagtcg agaagttccg agtctccgca
     4141 gaatcggatg cgccgggaca tcccgtgcct cgtgatgtac cgcaaccgga cttagccgcc
     4201 gcgacgtcat ttcggccacg gtggacgtcc gacgcgcatc ctgccgcagg ctgacaacag
     4261 gggcccgtcg tgacgatcaa ctcagtaccg acagcgagac ggaaccacca cgctgcgccg
     4321 gtgcacgaac cgccggcggc gatccgcgcc accgcgatcg tcgtggccct gacgacggcg
     4381 ttggcgatcc tcgcgatcgc gttcgcactg ccggccgccc gctccaagcc gcacgatatc
     4441 ccgatcggtg ctgccgggcc gcaggccgcg agcggacaag tcgccgacat cctcgagcag
     4501 cacgcgccag gcgcgttcgc actcacctat taccccggcg aggcggcgct gcgcgatgcg
     4561 atccgcaatc gggatgtcta cggcggcatg tcctttcgcc ccgatggccg cagcctgctg
     4621 atcgccaccg gcggtagccc ggtcgtcgcg cagatgctga cgcagatcgg caacggcgtt
     4681 gcgcagcaag caggcgtgcc gttgcacacc gaggacctcg ccccgccgac cgctgacgac
     4741 ccccgcggcg cgggcctggc cgcgtcggcg ctgccgatca cgctggccgg cctgctgccc
     4801 gctgtcgccc tggtgctgct ggtgcggcgc gaggtgtgga cgcgcctcgt cgccgcggtg
     4861 gtgttcgccg cgggggcggg cgtcacgatt gccgcgctgc tgcgctacgt cttcgggtcc
     4921 atcgaccaga acctgtgggg cgtcgcggcg gggctcaccc tgggactgct ggccgccggc
     4981 ctgtcggtgc tgggcctggg ctcgctgttc gggcgcggtg gactcgccgt cggcgcgctg
     5041 ctggccttgc tgctcggcaa cccgctgtcg ggcctgacca gcgcgccaga aatgctgccg
     5101 agcgggtggg gcacccttgg ccagtggctg ccacagggag ccaccgcgac gttgctgcgc
     5161 tccaccgcgt tcttcgatgg tgcaggcgcg gcgactgcga tcgcggcgct gacatgctgg
     5221 gcggttaccg gtacggcact cgtggtgatc gccgccgttc gccagggtag atcggtgccc
     5281 gcctaaactc gggcggcgtg ggactggacg accgcgactc gctgcgcata ctgcgcgacg
     5341 cgatcgatcc tgcgttgggg tccgacgagc tgatccgcga cttctacacc cgctggttcg
     5401 ccgtcgacat cacggcccgc gacctgtttc cgcccgatat ggatgtgcaa cgcgacgcgt
     5461 tcgccaaggc tgtgacgtgg ctgctgtccg aattgatcgc tcagcgggcg gatgagccgg
     5521 tggcgttcct cgctcagcta ggccgcgacc accgcaaata cgctgtgacg caacagcatt
     5581 acgacacgat gcaggatgcg ctgctcacta ccctccgcag tcatctgatc gcgcgctgga
     5641 acgacaggct ggccgcggcc gcgcaagacg cggtcacgct gatcatcggg gtgatgcggg
     5701 gctccgccga tgccgagaag ggaccgccgt accgcgacgg cacggtgatc gaacacgtgc
     5761 ggagcacccg cgatgtctcg gtgatccggc tacagctgga taagccgctg tactaccacc
     5821 ccggccagta cgtcacggtc caggtgccgc agtggccgcg acggtggcgt tacctgagcc
     5881 cggcgattcc ggccgaccgt ggcggagcga tcgagttcca catccgctcg gtcaccggcg
     5941 gcatggtcag cacggcgatc gtcggcgaga ccaagcccgg cgatcggtgg cggctgtcca
     6001 acccacacgg cggcatgcat gtcgaccgcg acggcgggga tgtgctgatg gtcgccggca
//
LOCUS       DASRJA010000322         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_532587,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000322 DASRJA010000000
VERSION     DASRJA010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532587"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VFC01_14070"
     CDS             <1..1094
                     /locus_tag="VFC01_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZN80793.1"
                     /translation="TPLDRSRPLWEMYFIEGLANGRIAVLGKIHHALADGVASANLLA
                     RGMDLQQGPQIDRDSYATDPAPTKGELLRSAFADHARLVGRLPGTVRYTAQGIRRVRQ
                     SSRKLSPELTRPFTPPPTFMNHKIDPVRRFATATLALADIKATGKHLGVTINDMVLAI
                     SAGALRKLLLRYDGHADNPLLASVPVSFDFSPDRISGNYFTGVLVALPTDCEDPLDRV
                     RNAREAALEAKESHNLLGPELVSRWSAYFPPKPAEAMFRWLSNKDGQNKVLNLPISNV
                     PGPREHGRVGGALVTEIYSVGPLTAGSGLNITVWSYVDQLNISVLSDGRTLDDPHELT
                     DEMINAFVEIRRAAGLPEELTVVESAMAQ"
     gene            complement(1117..2394)
                     /locus_tag="VFC01_14075"
     CDS             complement(1117..2394)
                     /locus_tag="VFC01_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN80794.1"
                     /translation="MRRRWPLLRASAELLNAANGVRPLGREGYITLPVFAFGWPTTEM
                     SPVYIAGSMLDAVRRGLRGDFRGRRGRIALVLTAIAWALLWLIHRRNVASEPQFEEPL
                     REALGDDYEKIAAQSQPARRRRMIGVWPNDLIRRRYVEKASTVQYGPHPRVNLADVWR
                     RGDLPRDGKAPVLLQVPGGAWAIGMRKPQAYPLLSHLAERGWVCVSIDYRVSPRHTWP
                     DHIVDVKRALAWIKENIADYGGDPDFVAITGGSAGGHLCSLAALTPDDAQYQPGFEDA
                     DTSVVAAVPIYGRYDWVSAKGDGRKEFIAFLQKFVVKKRFIEHKQVYVDASSIERVRP
                     DAPPFFILHGQDDSIIPVQEGRDFAEAMRQTSTSVVAYAEIPHAQHAFDFYYGSPRGH
                     YTARAVEEFLSWVHAKRNQASSGHLLHAKSPEA"
     gene            complement(2387..>3049)
                     /locus_tag="VFC01_14080"
     CDS             complement(2387..>3049)
                     /locus_tag="VFC01_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN80795.1"
                     /translation="GSRMRPDVVIRFMDQFGDVIYNNYNATEAGMIATATPQDLRAAP
                     DTAGKPAGGTEIRVLDDDFNEVPRGEVGSIFVRNSSQFDGYTSGKTKDFHEGFMSSGD
                     VGYLDDAGRLFVVGRDDEMIVSGGENVYPIEVEKTLAAHQEVAEAAVIGVDDEQFGQR
                     LAAFVVLADGATATPDTLKQHVRDNLANYKVPREITILDELPRGSTGKIVRRELQDRA
                     GA"
BASE COUNT          526 a         1009 c          992 g          522 t
ORIGIN      
        1 gcacaccgct ggaccgcagc cgtccgctat gggagatgta cttcatcgag ggactcgcga
       61 acggccggat cgcggtgctg ggcaagatcc atcacgcgct ggccgacggg gtggcgtcgg
      121 ccaacctgct ggcgcgggga atggacctgc agcagggtcc gcagatcgac cgtgactcct
      181 atgccaccga cccggcgccg acaaaaggcg agctcctgcg atcggcgttc gccgaccacg
      241 cccgactggt cgggcgatta ccgggcacgg tgcgctacac ggcgcagggg atacgccggg
      301 tccggcagag ctcacggaag ctgtccccgg agttgacccg tccgttcacc ccgccgccga
      361 cgttcatgaa ccacaagatc gatcccgtcc gccgattcgc gaccgcgacg ctagcgctcg
      421 cagacatcaa agcgactggc aaacacctgg gcgtgacgat caacgacatg gtgctggcga
      481 tctcggcggg cgcactgcga aaactgttgc tgcgctacga cggtcatgcc gacaatccgt
      541 tgctggcgtc ggtaccggtg agcttcgact tctcaccgga ccgcatctcg ggcaactact
      601 tcaccggggt gctggtggcg ttgccgaccg actgcgagga tccgctggat cgggtccgca
      661 acgcgcgcga agcggcgttg gaggccaagg aaagtcacaa cctgctcggc cccgaactgg
      721 tgagccggtg gtcggcgtac ttcccgccga agccggccga ggcgatgttc cggtggttgt
      781 ccaacaagga cggccagaac aaagtgctga acctgccgat ctccaacgtg ccgggaccgc
      841 gtgagcatgg ccgcgtcggc ggtgcgctgg taaccgagat ctattcggtg ggcccgctga
      901 cggcgggcag cgggctgaac atcaccgtgt ggagctatgt cgatcagctc aacatctcgg
      961 tgctgtccga cgggcgcacc ctcgacgacc cgcatgagct gaccgacgag atgatcaacg
     1021 cgttcgtcga aatccgccgt gcggcaggcc ttcccgaaga gctgacggtc gtcgaatccg
     1081 ctatggcaca gtaattctgt cgagtggcga gttatgtcac gcttcaggcg atttcgcgtg
     1141 caataggtgg ccactcgacg cctggttccg cttggcgtgc acccacgaca ggaactcttc
     1201 gacggcgcgt gcggtgtagt gcccgcgcgg cgacccgtag tagaagtcga acgcgtgctg
     1261 ggcgtgcggg atctcggcgt acgcaacgac ggacgtcgac gtctggcgca tcgcctcggc
     1321 gaagtcgcga ccttcctgca cagggatgat cgagtcgtct tggccgtgca gaatgaaaaa
     1381 cggtggcgca tcgggccgta cccgttcgat cgatgacgcg tcgacgtaca cctgcttgtg
     1441 ctcgatgaac cgtttcttca ccacgaactt ctgcaggaac gcaatgaact ccttgcggcc
     1501 gtcgcccttg gcggaaaccc agtcgtagcg gccgtagatc ggcaccgcgg ccaccaccga
     1561 ggtgtcggcg tcctcgaatc ccggttggta ctgcgcgtcg tcaggggtca gcgcagccag
     1621 cgagcacaga tgaccgcccg ccgagccgcc ggtgatcgcg acgaagtccg gatcgccgcc
     1681 atagtcggcg atgttctcct tgatccaggc cagcgcgcgc ttgacgtcga cgatgtggtc
     1741 cggccaggta tgacgcgggc tgacgcggta atctatcgac acgcacaccc agccacgctc
     1801 ggcgagatgg ctcagcagcg ggtacgcctg cggcttgcgc atgccgatcg cccacgcacc
     1861 gccgggcact tgcagcagga ccggcgcctt gccgtcccgg ggcaggtcgc cccgtcgcca
     1921 gacatcggcg aggttgaccc tcgggtgcgg gccgtactgg acggtgctgg ccttctccac
     1981 atagcggcga cggatcaagt cgttgggcca cacaccgatc atcctgcgac gccgggcggg
     2041 ttgcgactgc gccgcgatct tctcgtagtc gtcgcccaac gcctcgcgca gcggctcctc
     2101 gaactgcggc tccgacgcca cattgcggcg gtggatcagc cacaacagcg cccacgcgat
     2161 ggcggtgagc accaaggcaa ttcgccctcg gcggccgcgg aagtcgccgc gcagtccgcg
     2221 ccgcaccgcg tcgagcatcg acccggcgat atacaccggc gacatctcgg tggtgggcca
     2281 gccgaacgcg aacaccggca gcgtgatgta cccctcgcgg ccgaggggcc gcacaccgtt
     2341 ggcggcgttg agcagctccg ctgatgcgcg cagcagcggc cagcgcctac gcaccggcgc
     2401 ggtcctgcaa ttcgcggcgc acgatcttgc cggtgctgcc gcgaggtagt tcgtcgagga
     2461 tcgtgatctc cctgggcact ttgtaattcg ccaagttatc gcggacgtgt tgcttcagtg
     2521 tgtccggggt agccgttgcg ccgtcagcca aaaccacgaa cgcagccagc cgctggccga
     2581 actgttcgtc gtccacccct atcaccgccg cctcggccac ctcctggtgc gcggccagcg
     2641 tcttctccac ctcgatgggg tagacgtttt cgccaccgga gacgatcatc tcgtcgtcgc
     2701 ggccgacaac gaacagccga cctgcgtcgt cgaggtaacc gacatcgccg gacgacatga
     2761 agccctcgtg gaaatccttg gtcttgccag aggtgtaccc gtcgaactga ctggagttgc
     2821 ggacgaagat gctgccgacc tcaccgcggg gcacttcgtt gaaatcgtcg tccagaactc
     2881 ggatttcggt gccgccggca ggctttcccg cggtatcggg cgcggcccgc aggtcctgcg
     2941 gtgtcgccgt ggcaatcatg ccggcctcgg tggcgttgta gttgttgtag atgacgtcgc
     3001 cgaactgatc catgaacctg atgacgacat ccgggcgcat tcgtgaacc
//
LOCUS       DASRJA010000323         6489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3683631,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000323 DASRJA010000000
VERSION     DASRJA010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3683631"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..100
                     /locus_tag="VFC01_14085"
     CDS             <1..100
                     /locus_tag="VFC01_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80796.1"
                     /translation="AITVEEGGRVGDITEEAVDASIALLAPRVEPD"
     gene            136..684
                     /locus_tag="VFC01_14090"
                     /pseudo
     CDS             136..684
                     /locus_tag="VFC01_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(681..1628)
                     /locus_tag="VFC01_14095"
     CDS             complement(681..1628)
                     /locus_tag="VFC01_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80797.1"
                     /translation="MPTSKYGAATTAAEIVRDVDMHGRRAIVTGASSGIGVETARALA
                     STGADVTLAVRNPEAGTKVANAIAEKLPHGTGALQVAPLDLADPASVASFVRTWTEPL
                     HILVNNAGVMALPQLTRTPAGYEMQFATNHLGHFVLATGLHKWLAAADGARVVSVASI
                     GHLFSPVVFDDLHYRFRPYDQWTSYGQSKSANVLFAVGAAQRWADDGIAVNALMPGNV
                     ASTALARHMGADDLANFGETTALGLPPVKTVEQGAATSVLLAASPDLDGVTGRYYEDC
                     AVAPQVSERGTHTGGVAPYALDPNNAERLWQISTVLTTA"
     gene            complement(1656..2144)
                     /locus_tag="VFC01_14100"
     CDS             complement(1656..2144)
                     /locus_tag="VFC01_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN80798.1"
                     /translation="MESADFPRGDHAELSVREVADRLAIRELVDAYAFCADRRDAARQ
                     MALFTEDTDFVVYMDSRNLSPTQHLRGRAALRPVFEELNTYEATMHFNGQSTTVLDGD
                     DASAVTYCLAHHVKVDGSARSLMVAAIRYLDTFVKHDGTWFFRQRKLMVDWTETRPLT
                     IG"
     gene            2293..3105
                     /locus_tag="VFC01_14105"
     CDS             2293..3105
                     /locus_tag="VFC01_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN80799.1"
                     /translation="MEASRGLAAFLRARRELLKPADVGLPDSERRRVEGLRREEVAML
                     AGISTEYYLRLEQGRDHQPSNEVLEGLAHALQLDDDASSHLRELARPAPRRRTRSTPE
                     RFDPAVQTLIDSWPLTPTFVLNRNMTILASNPMARALSRFFTPGENLLRAAFLEPEMR
                     TLYCDWDKLTTRIVPFVRALLGADPPDRELVELIGELSIGSKRFATLWARYDVKHRTS
                     GPTAFYHPQVGQLDLHYTVLHLPEQRQMIVTYHADPGSPSEESLRLLASLTS"
     gene            3393..3845
                     /locus_tag="VFC01_14110"
     CDS             3393..3845
                     /locus_tag="VFC01_14110"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN80800.1"
                     /translation="MCLAEIVEFPFDTARNITNGLYTGFFQRHPGLRLILAHCGGALP
                     TLGWRIGEHTVMGGGPTDADIDPTHVAEVLQGLYYETALAGSRNSLLPTLEVTTADHV
                     LFGTDWPAAPEPTVVRNIANLTSFDGFTPEDLRRVERDNALRLFPRFA"
     gene            complement(3874..4362)
                     /locus_tag="VFC01_14115"
     CDS             complement(3874..4362)
                     /locus_tag="VFC01_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN80801.1"
                     /translation="MVASSDLSHRFEAESVSRALELVGERWTLLILREAFFGVRRFGQ
                     LARNLNIPRPTLSSRLRMLVDAGLLERVPYSRDPERHEYRLTDAGRDLFGAIVTLMRW
                     GDEHLPHPSGPPVVLRHNACGEIADPRLTCTHCGKEITAHNVTPEAGPGFRTDLVDEE
                     LA"
     gene            4449..5606
                     /locus_tag="VFC01_14120"
     CDS             4449..5606
                     /locus_tag="VFC01_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80802.1"
                     /translation="MNQPDLTTAPPSSASDIETVARQIAVTAGEKSFEIDKDRRLPEE
                     LVALLGESGLLRAGAPTEVRGLELPPGVALSCAEALARGNASAGWCVSIAITSSLLVA
                     YLPTSSREALFGDGRGVAAGVWAPRGTARTVDGGVVISGRWPFCSGITHADMMFAGCL
                     IDDQRAPSVVALRKEDLAVLDTWHTLGLRGTGSHDTVADEVFVPADRVFSIFNGPVVD
                     RPLYRFPVFGFFALSIGAAALGNARGAIDDLIELACAKKGLGSTRTLAERPATQAAVA
                     TAESALSAAQALYYQAIETAWQASQDSEGVSVATRNRLRLAATHATRTAADVVRDMYD
                     LAGGTAIYDNSPLQRRFRDAYTATAHFQVNEASRELPGRILLDQQADVAML"
     gene            5603..>6489
                     /locus_tag="VFC01_14125"
     CDS             5603..>6489
                     /locus_tag="VFC01_14125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN80803.1"
                     /translation="MTGRIEVALPFWLDRPDEEALDVAMAARDARFDTLWIGEMATYD
                     AFALATAIGHRVPGLHLKLGPLALGVRSPVTLALGASSVASLAGSRVDVALGASSPAI
                     VAGWHDRQWAQHASRMRETIECLRSILAGKRVDYTGRHVRCHGFRLRKPMLDSRIAVG
                     AFGPAMTRVAAQHADEVVLNLTPPARVAGMRAIIDAEAAAAGRIAPRLTVWVPVAVNP
                     GDAARDQLSAQLAVYLAPPGYGEMFSELGFLDLVQRARGGAKRAELAAAVPVELLDQV
                     CALGTPEGIIARIQAYHDAG"
BASE COUNT         1096 a         2123 c         2153 g         1117 t
ORIGIN      
        1 cgcgatcacc gtcgaggagg gcggccgggt gggcgacatc accgaggaag cggtggatgc
       61 gtcgatcgcg ctgctggctc cgcgcgttga gcccgattag tcggcgacgg tgatcgcctg
      121 cgtcgggcac gagtcggcgg catcgcggcg atccagctgg ccaaggcggc gggtgccgag
      181 gtgcatggca cggcctcgcc cggcaagcac aagcgactgg cagagctcgg cgtcgatctc
      241 gccatcgact atcgccgcga tggttggtgg aaaggcttgc cgccgtacga cattgtgctg
      301 gatgccatcg gaggcaagtc cctgaagcga tcctatgagt tattgcgtcc gggcggccgg
      361 cttgtaggtt acggagcttc ggcgatgcaa cagggcgaga agcgatcgct tcgcaccgcg
      421 ctaccgcaac tgttgtccat ggtgcgcggt ttcaacctga tcaagcagcc gtcggaatcc
      481 aagacgttga tcggtctgaa catgcttgcg ctgagggacg accgcggcac actgcagccg
      541 tggatcgagc cgctgtcaac cgcgctcgcc gacggcatcg tccagcctgt ggtccacgcg
      601 gcggtaccct tcgccaacgc cggtgaggcc caccgcatcc tggcggcgcg cgagaacgtc
      661 ggcaaggtcg tgttggtgcc ctaagccgta gtcagcacgg tcgagatctg ccagaggcgc
      721 tcggcgttgt tcgggtcgag tgcgtatggt gccacgccac cggtgtgagt gccgcgctca
      781 ctgacttgtg gcgccacagc gcagtcctcg tagtaacggc cggtgacgcc gtcgagatcg
      841 ggactcgccg ccagcaagac cgacgtagca gcgccttgct cgactgtctt cactgggggc
      901 agccccaagg ctgtcgtttc gccgaagttg gcgaggtcgt ccgctcccat gtgtcgcgcg
      961 agcgcggtgc tggcgacgtt gccaggcatc aatgcgttga ctgcaatgcc gtcgtcggcc
     1021 cagcgttgcg ccgcgccgac ggcgaaaagc acgttcgccg acttggattg gccgtaggac
     1081 gtccattggt cgtaggggcg aaatcggtag tgcaggtcat cgaacacaac cggggagaac
     1141 aggtgtccga tggaggcgac ggagacgaca cgcgcgccgt cggctgcggc caaccacttg
     1201 tgcaggccgg tggccaagac gaaatgaccg agatggtttg tggcgaactg catttcatac
     1261 ccggccgggg tacgggttag ctgtggcaac gccattacgc ctgcattgtt gacgaggatg
     1321 tgcagcggtt cggtccacgt gcgcacgaag cttgccaccg acgccggatc ggccaggtcg
     1381 agcggggcga cctgcagcgc tcccgtcccg tgcggcagct tctcggcaat agcgttcgcg
     1441 actttcgtgc cggcctcggg gttgcggacc gccagcgtca cgtcggcgcc ggttgatgcg
     1501 agcgcgcggg ccgtttcgac accgattccc gagctggcgc ccgtcacgat ggcgcgtcgt
     1561 ccgtgcatat cgacgtcccg gacgatctcg gcggccgtgg tggcggctcc gtatttgctc
     1621 gtaggcatgg cggtctcctt cgaggattca ccggattaac cgatcgtcag tgggcgggtt
     1681 tcggtccaat cgaccatgag tttgcgctgg cggaaaaacc aggtgccgtc gtgtttgacg
     1741 aacgtgtcga gatagcggat cgctgcgacc atcaggctgc gtgccgaccc gtcgaccttg
     1801 acgtggtggg ccaggcagta ggtcaccgct gaggcatcgt cgccatccag caccgtggtg
     1861 ctttgtccgt tgaaatgcat ggtggcttcg taggtgttga gttcttcgaa caccggcctg
     1921 agggcggccc gcccgcgtag gtgttgggtc ggcgagagat ttctgctgtc catatagacg
     1981 acaaagtccg tgtcctcggt gaacagagcc atttggcggg cagcgtcacg acgatcggcg
     2041 caaaacgcgt acgcgtcgac aagttcgcgg atcgccagcc tgtctgctac ttcccgaacg
     2101 gatagctcgg cgtggtcgcc gcgggggaaa tccgcagatt ccaaggttct tcccgtcttc
     2161 gagcgccggc tggcgatccg ttcatttccg atgcgaccac gatgcgctcc cacgcaccgg
     2221 gtatccaggc cacatcgttg ctggtaatcc gagggtcacc ctttctacgg cgacgcgtcc
     2281 tacccttgat cgatggaagc gtcccgtggg ctggcggcgt tcctgcgcgc ccgccgcgaa
     2341 ttgctcaagc ccgccgatgt cggcctgccg gacagcgagc gcagacgcgt cgaaggtttg
     2401 cgtcgggaag aagtcgcgat gcttgccggc atcagcacgg agtactacct gagactcgag
     2461 caaggacgag atcaccagcc ctccaacgaa gttcttgaag gacttgccca cgccctccaa
     2521 cttgacgacg acgccagcag tcacttgcgc gagttagcgc gcccagcacc gcgtcggcgc
     2581 acgcgctcga cccccgaaag gttcgacccg gccgtgcaaa ctctcatcga cagctggccg
     2641 cttacgccta cgttcgttct caacaggaac atgacgatac tggcgtccaa ccccatggcc
     2701 cgagcactgt cgcgcttctt cacaccgggt gaaaaccttc tgcgcgcagc gtttctcgag
     2761 ccggaaatgc gcacgctcta ctgtgactgg gacaaactca ccaccaggat tgtcccgttc
     2821 gtgcgagccc tactcggcgc cgacccgccc gatcgcgaac tggtggaact catcggcgaa
     2881 ctcagcatcg gcagcaagcg cttcgccaca ttgtgggcgc gttacgacgt caaacatcga
     2941 actagcggtc cgaccgcctt ttaccacccg caagtcgggc agctcgatct gcattacacg
     3001 gtcctgcact taccggagca acgtcagatg atcgtcacct accacgccga tcccggttcg
     3061 ccgtcggagg aaagcctgcg cctgctcgcc agcttgacga gctagggcaa ttgtccgcct
     3121 agtcccgcgg aatcgtaggc gtgtcatggt ctgccagggc ggcgtacgcc cagtggtcgt
     3181 ccagcatggt cgattcggca aggagcgtca ggacggctcc tcgacagcct ccgcgctcca
     3241 aatgccgatg tcttccttac ccatcggttc ggtacatgtc aggcagccga gaaccggatc
     3301 ggccgactgt ccgcacagag tgtgtgtcag cctgcacagc gtgtagcccg aggccggtca
     3361 actccagcca atggtcggga cgctcgccgg ccttgtgttt ggccgagatc gtcgagttcc
     3421 ccttcgacac tgcgcgcaac atcaccaacg ggctgtacac cgggttcttt caacggcatc
     3481 cggggctacg gctgatcctg gcccactgtg gcggggccct gccgacgctg ggatggcgca
     3541 tcggcgaaca caccgtgatg ggcggcggac ccaccgacgc tgacattgac ccgacccatg
     3601 tcgccgaggt gctgcagggg ctgtactacg agacggcatt ggcaggcagc cggaactcgc
     3661 tgctgccaac gttggaggtg accaccgcag accatgtcct cttcggcacc gactggcccg
     3721 ccgccccgga gcccactgtg gtccgaaaca tcgccaacct caccagtttc gacggcttca
     3781 cccccgagga cctacgtcgc gtggagcgcg acaacgccct gcgcctgttt ccccgattcg
     3841 cgtgactgcg gtggaggcca gcgtgactgc cgatcaggcc agctcttcgt cgaccaaatc
     3901 ggtgcggaag ccggggccgg cctcaggggt gacgttgtgt gcggtgatct ccttgccgca
     3961 atgggtgcac gtgaggcgcg ggtcggcgat ttcaccacag gcgttgtggc gcagcacgac
     4021 cggtgggcca ctcgggtgcg gcagatgctc gtcgccccac cgcatcaggg tcacgatcgc
     4081 tccgaagaga tcgcgcccgg cgtcggtgag ccggtattcg tggcgctcgg gatcgcggga
     4141 atacggaacc cgctcaagca ggccagcgtc gaccagcatc cgcagccggg acgagagcgt
     4201 gggccgcggg atgttgaggt tgcgcgcgag ctgaccgaag cgccgcaccc cgaagaacgc
     4261 ttcgcgcagg atcagcagcg tccaccgctc gccgaccagc tcgagcgctc ggctgacgga
     4321 ctcggcttcg aagcggtggg acagatcaga ggacgcaacc atggttcaat aatcatactg
     4381 gttaagaaac tgaaccggta gtggtaacgt gactgaacca gtccgcccac acgaatgctg
     4441 agatcgccat gaaccagccc gacctgacga cagcaccgcc gagcagtgcg agcgacatcg
     4501 agaccgtcgc ccggcagatc gctgtgacgg cgggggagaa gtccttcgag atcgacaagg
     4561 accgccggct acccgaggag ttggtggcgc tcctcggaga gtctggactg ctacgcgccg
     4621 gtgcgccgac ggaagtccgc gggctggagt tgccgccggg agtggccctg tcgtgcgcgg
     4681 aggcgctggc ccgcggcaac gcctcggcgg gctggtgcgt ctcgatcgcg atcaccagca
     4741 gcctgctggt cgcgtatctg ccgacgtcca gccgcgaagc gctcttcggt gacgggcgcg
     4801 gagtagcggc aggcgtgtgg gcgccgcgcg gtacagcgcg gacggtcgat ggcggcgtcg
     4861 ttatctcggg acgctggccg ttctgcagcg gaatcaccca cgcggacatg atgtttgccg
     4921 gctgcctgat cgacgatcag cgcgcgccgt cggtcgtcgc gctacgcaaa gaagacctcg
     4981 cggttctcga cacctggcac acactgggcc tgcgcggcac cggcagtcac gacaccgtcg
     5041 ccgacgaggt cttcgtgccg gccgatcgcg tgttctctat attcaacggt ccggtcgtgg
     5101 accgaccgct gtatcgcttc ccggtgttcg ggttcttcgc gctgtcgatc ggcgcggccg
     5161 cgctgggcaa tgcgcgcggc gcgatcgacg acctcatcga gttggcctgc gccaagaaag
     5221 ggctcgggtc gacccgcaca ctggccgagc gacccgcaac ccaagcggcg gtggccacag
     5281 ccgagtcggc gctgagcgct gcgcaagcgc tgtactacca ggcgatcgaa actgcgtggc
     5341 aggccagcca ggacagcgaa ggcgtgtccg tcgcgacgcg aaaccgcctg cgtctggcgg
     5401 caactcacgc gacgcgtacc gccgccgatg tggtccgcga catgtacgac ctcgccggcg
     5461 gcaccgcgat atacgacaac tccccactac agcgacgctt ccgcgacgcg tacaccgcca
     5521 ccgcccattt ccaagtcaac gaggcgtcgc gcgaactgcc cggccgcatc ctgctcgacc
     5581 agcaggccga cgtggcgatg ctgtgaccgg acgcatcgaa gtcgccctgc cgttctggct
     5641 ggaccggccc gacgaggagg cgctcgacgt cgcgatggcg gcccgcgacg cccggttcga
     5701 cacgttgtgg atcggtgaaa tggccaccta cgacgccttc gcgttggcca ccgcgatcgg
     5761 tcaccgcgtg cccggcttgc acctgaagct cggaccgctg gcacttggtg tccgcagccc
     5821 ggtaactctg gcgttgggtg catcgtcggt cgcatccctc gccggcagca gagtcgacgt
     5881 cgcgctgggc gcctccagtc cggcgatcgt ggccggctgg cacgaccgcc agtgggcgca
     5941 gcacgcgtcg cgcatgcgcg aaacgatcga gtgtctgagg tcgatcctgg ccgggaagcg
     6001 ggtcgactac accggacgcc acgttcgctg ccatggcttc cggctgcgaa agccgatgtt
     6061 ggacagcaga atcgcggtcg gcgcgttcgg acctgcaatg acgcgggttg ctgcgcagca
     6121 cgcagatgag gtcgtactca atctcactcc gcccgctcgg gtcgccggca tgcgggcgat
     6181 catcgacgcc gaagcggccg cggccgggcg aatcgcgccg cgcttgacgg tgtgggttcc
     6241 ggtcgcagtc aaccccggcg acgcggcgcg cgatcagctg tcggcccagc tggccgtcta
     6301 tctggcccct cccggatacg gcgaaatgtt cagcgagctc ggctttctcg acctcgtcca
     6361 gcgcgcccgc ggtggtgcaa agcgcgccga gctggcggcc gccgtccccg tcgagctatt
     6421 ggaccaggtg tgcgccctgg gcacgccgga gggcatcatc gcgcggatac aggcctacca
     6481 cgacgccgg
//
LOCUS       DASRJA010000324        13897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1468858,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000324 DASRJA010000000
VERSION     DASRJA010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13897
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468858"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFC01_14130"
     CDS             <1..651
                     /locus_tag="VFC01_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZN80804.1"
                     /translation="RTTGTYLAAATIGTDADRDLIRTEVDRVHALIRSVESSPVSYNA
                     FDPRLQLWVAACLYRYYVDQHEFLYGPLDDDSADAVYQDAKTLGTTLQVRDDMWPPDR
                     VAFDEFWKRSLDDLRIDPPVREHLRGVAAMAFLPAPLRLLAGRFNLFATAGFLPPEFR
                     SQMRLSWTAGQQRSFEWLLRALRLADRVIPRDVWILGYQLYLRDMRARARRGRPIV"
     gene            715..1110
                     /locus_tag="VFC01_14135"
     CDS             715..1110
                     /locus_tag="VFC01_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN80805.1"
                     /translation="MAFPALNHVALTVRDLGVSAPWYRSLIGADPVLDEHTDAGFRHL
                     VWAFDNGTLFGIHQHDRDIEGGQFTEFRAGLDHVSFGCADRAELEEWAEKLEGLGIEH
                     GRVVDAHYGSGVSFRDPDGNALEFFAPPE"
     gene            complement(1115..1186)
                     /locus_tag="VFC01_14140"
     tRNA            complement(1115..1186)
                     /locus_tag="VFC01_14140"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1152..1154),aa:Val,seq:tac)
     gene            complement(1231..1695)
                     /locus_tag="VFC01_14145"
     CDS             complement(1231..1695)
                     /locus_tag="VFC01_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZN80806.1"
                     /translation="MLDVGTEAPDFTLKDQNGQLVTLSNFRGVKDVLLVFFPLAFTGI
                     CQGELDEIRDDLPSYQNDDTVTLAISVGPPPTHKVWATQSSFTFPVLSDFWPHGAVAQ
                     SYGVFNDDTGFANRGTFVVDRSGIILFAEMKQPGEQRDQAVWTDALKAMRSG"
     gene            complement(1695..2120)
                     /locus_tag="VFC01_14150"
     CDS             complement(1695..2120)
                     /locus_tag="VFC01_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HZN80807.1"
                     /translation="MVAADDAPSYARRLGIQKDQVVQELGWDSDVDDDIRADIEEACG
                     GELLDEDADEVIDVVLLWWRDGDGDLVDQLMDAITPLADDGVIWVVTPKTGQPGHVQP
                     AEIAESAPTAGLMQTSSANLGDWIASRLVQPKSKAAGRR"
     gene            complement(2224..2778)
                     /locus_tag="VFC01_14155"
     CDS             complement(2224..2778)
                     /locus_tag="VFC01_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80808.1"
                     /translation="MRLRIAALAVGSMAAVAGIIVGCTSVTQGTAEIDRADAPAYRAS
                     VSASAEESSASSSSRESERQASLTTQAIHSSCEALSSSSVDAITAVNNYVDAFNRNAA
                     DAGAKAPPAIDALNHSADLVARSVSDALRADLRDALNAWVDAARGLASAIAGNYGPDE
                     FNAAITKLNDTKTTALNLCDAAYR"
     gene            2950..5739
                     /gene="aceE"
                     /locus_tag="VFC01_14160"
     CDS             2950..5739
                     /gene="aceE"
                     /locus_tag="VFC01_14160"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HZN80809.1"
                     /translation="MTTEFVRQDLAQNSSTTAEPDRVRVIREGVASYLPDIDPEETAE
                     WLESFDELLERSGPARARYLMLRLLERSGEQRVAIPALTSTDYVNTIPTELEPWFPGD
                     EDVERRYRAWIRWNAAIMVHRAQRPGVGVGGHISTYASSAALYEVGFNHFFRGKAHPG
                     GGDQVFIQGHASPGVYARAFLEGRLTADQLDGFRQEHSHPGGGLPSYPHPRLMPDFWE
                     FPTVSMGLGPMNAIYQARFNHYLHDRGLKDTSDQHVWAFLGDGEMDEPESRGLIQVGA
                     NEGLDNLTFVINCNLQRLDGPVRGNGKIIQELESFFRGAGWNVIKVVWGREWDALLHA
                     DRDGALVNLMNTTPDGDYQTYKANDGAYVRDHFFGRDPRTKALVADMSDAEIWNLKRG
                     GHDYRKVYAAYRAATEHKGQPTVILAKTIKGYSLGAHFQGRNATHQMKKLALEDLKYF
                     RDAQRIPVSDAELEENPYLPPYYHPGPEAPEIRYLLERRRALGGFLPERRTKSKVLTL
                     PGRDTYKALKKGSGTQEVATTMATVRTFKELLRDKEIGKRIVPIIPDEARTFGMDSWF
                     PSLKIYNRNGQLYTAVDAELMLAYKESEVGQILHEGINEAGSTSSFTAVGTSYATHNE
                     PMIPVYIFYSMFGFQRTGDGFWAAADQMARGFVLGATAGRTTLTGEGLQHADGHSLLL
                     AATNPAVVAYDPAFAYEIAYIVESGLHRMYGENPENIYFYVTIYNEPYVQPAEPEGVD
                     VEALLRGIYRYRMAPEKRTHVAQILASGVAMPEAVRAADLLAEEWDVAADVWSVTSWG
                     ELNRDGVAIEKERLRHPERPAGTPFITNALADAAGPVIAVSDWMRAVPEQIRPWVPGT
                     YITLGTDGFGFSDTRPAARRYFNTDAESVVVGVLEGLARDGNIDQSVAVEAARKYRID
                     DVMAAPEQTSDPGVA"
     gene            5792..7078
                     /locus_tag="VFC01_14165"
     CDS             5792..7078
                     /locus_tag="VFC01_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HZN80810.1"
                     /translation="MTDNRFIPPKSTLEVLETVPESVLRRLSNYSGRLATEAVRAMEE
                     RLPFFANLEASQRASVQLVVQTAVVNFVQWMRDPRSNVSYTAQAFEVVPQDLRRRIAL
                     RQSVEMVRVTMEFFEEWVPVLARSEEQVTAMTAGILRYSRDLAFAAATAYADQAEARG
                     AWDTRMEANVVDAVVRGDTGPELQSQAAALNWDATAPATVIVGLPQPDRIDLTSDDVH
                     DVVTRNGRAALSDVHGTWLVAIVSGPLAPTDRFLNELLGVFADGPVVIGPTAPTLGAA
                     HISATEAIAGMNAVAGWSGAPRPVSARELLPERALLGDATAVAALEAEVMRPLADAGP
                     ALTETLDAYLDSGGAIEACARKLFVHPNTVRYRLKRIADFTGRDPTLPRDAYVLRVAA
                     TVGRLGRHAYHPSTSNASSEVVKSDHQRPQGVHRAG"
     gene            7207..8124
                     /locus_tag="VFC01_14170"
     CDS             7207..8124
                     /locus_tag="VFC01_14170"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZN80811.1"
                     /translation="MLALLAPGQGSQTPGMLAEWLELPGAAERLAAWSQISGLDLVRL
                     GTTATAEEITDTAVTQPLVVAATLLAHEELTRRGSLADKETVVAGHSVGEIAAYAIAG
                     VISADDAVKLAATRGAEMAKACALEPTGMSAVLGGDEAEVLARLEALDLVPANRNAAG
                     QIVAAGALSALDKLAEDPPAKARVRQLATAGAFHTHFMASATDGYASAVEKVTAAESL
                     RPTAKLLSNADGQPVASAADAMTKLVAQLTRPVRWDLCTETMRQSNVTAIVEFPPAGT
                     LAGIAKRELRGVPTHAVKSPAELDGLAEL"
     gene            8213..8512
                     /gene="acpM"
                     /locus_tag="VFC01_14175"
     CDS             8213..8512
                     /gene="acpM"
                     /locus_tag="VFC01_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="meromycolate extension acyl carrier protein
                     AcpM"
                     /protein_id="HZN80812.1"
                     /translation="MATTQEEIIAGIAEIIEEVTGIEPSEVTPEKSFVDDLDIDSLSM
                     VEIAVQTEDKYGVKIPDEDLAGLRTVGDVVAYIQKLEEENPEAAAAIRAQIAETK"
     gene            8509..9759
                     /gene="kasA"
                     /locus_tag="VFC01_14180"
     CDS             8509..9759
                     /gene="kasA"
                     /locus_tag="VFC01_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase KasA"
                     /protein_id="HZN80813.1"
                     /translation="MTKPSTANGGFPSVVVTAVTATTSIAADIESTWKGLLAGESGIR
                     VLEDEFVTKWDLPVRIGGHLVDSVDDHMTRLDLRRMSYVQRMAKLLGGQVWESAGSPE
                     VDPDRFAVVIGTGLGGGEKIVETYDAMNEGGPRKVSPLAVQMIMPNGAAAVVGLQLGA
                     RAGVITPVSACSSGSEAIAHGWRQIVMGDADIAVVGGVEGGIEALPIAAFSMMRAMST
                     NNEDPAGASRPFDKNRDGFVFGEAGALLVIETEEHAKARGAKPLARLMGAGITSDAFH
                     MVAPASDGLRAGHAMKRAMETAGLDPKDIDHVNAHATATPIGDTAEANAIRVAGVEHA
                     PVYAPKSALGHSIGAVGALESVLTVLTLRDGVIPPTLNYETPDPEIDLDVVAGEPRYG
                     EYQYAINNSFGFGGHNVALAFGRY"
     gene            9784..11037
                     /gene="kasB"
                     /locus_tag="VFC01_14185"
     CDS             9784..11037
                     /gene="kasB"
                     /locus_tag="VFC01_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase KasB"
                     /protein_id="HZN80814.1"
                     /translation="MGGLATGNGFPNVVVTGIAMTTALATDAESTWQKLLDGQSGIRK
                     LDDPFVEQYDLPVRIGGHLLEDFDSELTRIELRRLSYLQKMSTVLGRRAWQNAGSPEV
                     DSRRLMVSIGTGLGSTEELVFAYDDMRAKGMRAVSPLAVQKYMPNSPAAAVGLERGAK
                     AGVITPVSACASGSEGIAHAWQQIVLGEADIAVCGGVESRIEAVPIAGFAQMRIVLST
                     NNDDPAGACRPFDRDRNGFVFGEAGALLVIETEEHAKARGANILARVMGASVTSDGYH
                     MVAPDPNGEQAGHAMTRAIQLAGLAPTDIDHVNAHATGTSVGDVAEGHAINNAMGGHR
                     PAVYAPKSALGHSVGAVGAVESILTILALKNGIIPPTLNLKNLDPEIDLDVVHGGPRP
                     GNYQYAVNNSFGFGGHNVALAFGKY"
     gene            11103..12524
                     /locus_tag="VFC01_14190"
     CDS             11103..12524
                     /locus_tag="VFC01_14190"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HZN80815.1"
                     /translation="MTIMAPEVVGESLDPRDPLLRLSTFFDDGSVELLHERDRSGVLV
                     AAGTVNGVRTIAFCTDGTVMGGAMGVEGCAHIVNAYDTAIEEQSPIVGIWHSGGARLA
                     EGVKALHAVGLVFEAMIRASGYVPQISVVVGFAAGGAAYGPALTDVVVMAPEGRVFVT
                     GPDIVRSVTGEDVDMASLGGPDTHHKKSGVCHIVADNELDAYERGRRLVGLFCQQGHF
                     DRDKAEAGDTDLHALLPDSARRAYDVHPLVDALLDDDAPLEEFQAKWAPSMVVGLGRL
                     SGRTVGVLANNPLRLGGCLNSESAEKAARFVRLCDAFGIPLIVVVDVPGYLPGVDQEW
                     GGVVRRGAKLLHAFGEATVPRVTLVTRKIYGGAYIAMNSRSLNATKVFAWPDAEVAVM
                     GAKAAVGILHKRALAAAEDHEREALHDELAAEHERIAGGVDSAIEIGVVDEKIDPAHT
                     RSKLTQALAEAPARRGRHKNIPL"
     gene            12545..12937
                     /locus_tag="VFC01_14195"
     CDS             12545..12937
                     /locus_tag="VFC01_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80816.1"
                     /translation="MARTKNIFDYASKVLSATAFWAWVLDAENADDATLNAINADLRS
                     KLALPQQARLVDVVLAKNPDDPRLMPHLPSEEGERDDRKRIDILATYRLPERGLLDLS
                     LDRQQPTHRFAVIARRRGRIAAAQGARR"
     gene            13039..>13897
                     /locus_tag="VFC01_14200"
     CDS             13039..>13897
                     /locus_tag="VFC01_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HZN80817.1"
                     /translation="MDTEGLRPADLRRWRQHLANERAEAGVYRHLAQRRTGEERDILL
                     TLADAERRHEQHWLNLLGDHVGKPRRADLRTRMLSFLARHFGSLFVLALAQRAEARSA
                     YDTDVDATPTMAADERIHLEVVRALATRGRNQLSGSFRAAVFGANDGLVSNLALVLGI
                     SATGVTNHTVLATGLAGLLAGALSMGAGEYVSVNSQRELLEASTPSPEAGAALPQLDV
                     NANELALVYRARGMSAEEATGHAAKVLNNLATGGSHSRIARPDPDQHEAVGTGLRAAI
                     SSFSFFASGA"
BASE COUNT         2446 a         4552 c         4611 g         2288 t
ORIGIN      
        1 cgcaccaccg ggacctacct cgccgccgcc acgattggca ccgacgcgga ccgagatttg
       61 atccgcactg aagtcgaccg ggtgcacgca ctgatccgct cggtggagtc aagcccggtg
      121 tcctacaacg cattcgatcc acggctgcag ctttgggtgg ccgcctgcct gtaccgctac
      181 tacgtcgacc aacacgagtt cttgtacggg ccgctcgacg atgactccgc cgatgcggtg
      241 tatcaggatg cgaagacact gggcaccacg ctgcaggtcc gcgacgatat gtggccgccc
      301 gaccgggtcg cgttcgatga attctggaaa cggtcgctcg acgaccttcg gatcgaccca
      361 ccggtgcgcg aacatctgcg cggagttgcc gcgatggcgt tcctacccgc cccgctgcga
      421 ctgctggccg ggcgattcaa cctgttcgcg accgcagggt tcctgccgcc ggagttccgc
      481 tcacagatgc ggctgtcctg gaccgccggt caacaacgct cgttcgaatg gctgctgagg
      541 gcgctgcggc tggccgatcg ggtgattcca cgtgacgtct ggatcctcgg ctaccagctg
      601 tatctgcgcg atatgcgggc ccgtgcgcgc cgcggcagac caatcgtgtg aaccgggcgc
      661 cgcccggcca cgtatcaagc acgacttcgc aatgtgaaca gtaaggagta acgaatggct
      721 ttcccggccc tcaaccatgt cgcgctcacc gtgcgcgatc tcggcgtcag cgccccctgg
      781 taccgatcct tgatcggtgc tgaccccgtg ctcgacgagc acaccgatgc aggtttccgg
      841 catctggtct gggcgttcga caacggcacg ctcttcggca tccaccagca cgaccgcgat
      901 atcgaaggtg ggcaattcac ggagttccgg gccggcctcg accatgtcag tttcggctgc
      961 gccgaccgcg ccgaactgga ggaatgggcc gagaagctcg agggcctcgg catcgaacac
     1021 gggagagtcg tcgatgcgca ctacgggtcg ggcgtgagct ttcgcgaccc cgatggcaat
     1081 gcgttggagt ttttcgctcc gccggagtga tcggtgggcg cggacggtat cgaaccgccg
     1141 accgctggtg tgtaaaacca gagctctacc actgagctac gcgcccttgc cgggcaggct
     1201 acaggccgat gacctgcagg gggaaatccg tcaacccgac ctcatcgctt tgagcgcatc
     1261 tgtccacacg gcctgatcgc gctgctcccc gggctgcttc atctcggcga acaggatgat
     1321 gcccgagcga tcgacgacga acgtgccgcg gttggcgaag ccggtgtcgt cgttgaacac
     1381 gccgtaggac tgcgcgaccg ccccgtgcgg ccagaagtcg gacagcacgg gaaaggtgaa
     1441 gctgctctgc gtcgcccaga ccttgtgcgt cggcggcgga ccgacagaga tcgccagcgt
     1501 gacggtgtcg tcgttctggt aggagggcag gtcgtcgcgg atctcgtcca gctcgccctg
     1561 gcagatgccg gtgaatgcaa gcgggaagaa caccagcagc acatccttga ctccgcggaa
     1621 gttgctcagc gtgacgagct ggccgttctg gtccttcagc gtgaagtccg gcgcctcggt
     1681 gccgacgtcc agcactaccg gcgcccggca gctttcgact tcggctgcac cagccggctg
     1741 gctatccagt cgccgaggtt ggccgacgac gtctgcatca ggccggccgt cggcgccgat
     1801 tcggcgatct cggcgggctg cacatggccg ggctggccgg ttttcggggt gactacccag
     1861 atgacgccgt cgtcggccag cggtgtgatg gcatccatca gctggtcgac gaggtcgccg
     1921 tcgccgtcac gccaccacag caacacgacg tcgatgacct cgtcggcgtc ctcatcgagc
     1981 agctcgccgc cgcacgcctc ttcgatatcg gcccggatgt cgtcgtcgac gtcggagtcc
     2041 cagcccaact cctgcacaac ctgatctttt tggatgccca gtctgcgggc gtagctcggg
     2101 gcgtcatccg ccgcgaccac ggtcggtcct ccttcagccg gctcgggcgc acgatgcgct
     2161 cggcgatgtc ctgagcccta tcgtcgcacg ccgagaccta agaccgatgc atggcggcca
     2221 ggttcatcga tatgccgcgt cacagaggtt cagcgccgtg gtcttggtgt cgttcagctt
     2281 ggtgatcgcg gcgttgaatt cgtcgggccc gtagttgcct gcgatcgcgc tcgccagccc
     2341 ccgtgcggcg tcgacccagg cgttgagcgc atcgcgcagg tcggcgcgca gcgcgtcgga
     2401 gacactgcgg gcgaccaggt cggcgctatg gttgagcgca tcgatggccg ggggcgcctt
     2461 ggcgcccgcg tcggcggcgt ttcggttgaa ggcgtcgacg tagttgttca ccgcggtgat
     2521 ggcgtcgacg cttgacgaac tcaacgcttc acacgatgag tggatcgcct gcgtggtcag
     2581 cgacgcctgg cgctccgatt cacgggagct cgagctggcg gacgactcct ccgccgacgc
     2641 cgacaccgag gcccgatacg caggcgcatc ggcgcggtcg atctcggctg taccttgagt
     2701 gacgctggtg caaccgacga tgatccccgc caccgcggcc atgctgccca cggccagggc
     2761 ggcgatccgc agccgcattc ggcccagccc accggtcagc acggtctgca gacgttaccg
     2821 cctcacgccg ctgatcgtct gttcattcca ctcggcacac ccggcggtgc aagtgcggca
     2881 ggatgggaca aagatggagg cagcttcgcc gatcgcggag cacctacacc gcctaacaag
     2941 gagcggaagt tgaccaccga gttcgtgcgc caagacctgg cccaaaactc cagcaccaca
     3001 gccgaacccg atcgggttcg ggtgattcgt gaaggcgtcg catcgtatct gcccgatatc
     3061 gatccggagg agaccgccga atggctggag tcgttcgacg agttgcttga gaggtcgggg
     3121 cctgcgcggg cgcgctacct gatgttgcgg ttgctggaac gctctggcga acagcgggtg
     3181 gccattcccg cgctgacgtc gaccgactac gtcaacacca tcccaaccga actcgagccg
     3241 tggtttcccg gcgacgaaga cgtcgagcga cgctatcggg catggatccg ctggaacgcc
     3301 gcgatcatgg tgcaccgggc gcagcgcccg ggagtcggtg tgggaggcca tatttcgacg
     3361 tacgcgtcct cggcggcgct ttacgaagtc ggcttcaacc atttcttccg cggtaaggca
     3421 catccgggcg gcggcgatca ggtattcatc cagggccacg cgtcgccggg cgtctatgcc
     3481 cgcgcatttc tcgagggccg gctaaccgcc gaccaactcg acggtttccg tcaggagcac
     3541 agccaccccg gcggcgggct gccctcctac ccccatccgc ggctgatgcc ggacttctgg
     3601 gagtttccca cggtgtcgat gggtctcggc ccgatgaacg cgatctacca ggcccggttc
     3661 aaccactacc tgcacgaccg gggcctcaag gacacctccg accagcacgt gtgggcgttc
     3721 ctcggcgacg gtgagatgga cgagccggag agccgcgggc tgattcaggt gggcgccaac
     3781 gagggtctgg acaacctgac cttcgtgatc aactgcaacc tgcagcgcct cgacggtccg
     3841 gtccgcggca acggcaagat catccaggag ctggagtcgt tcttccgggg cgccggctgg
     3901 aacgtcatca aggtggtgtg gggccgcgaa tgggatgccc tgctgcatgc cgaccgcgac
     3961 ggtgcgctgg tgaacctgat gaacaccacg cccgacggtg actaccagac ctacaaggcc
     4021 aacgacgggg cctacgtccg cgaccatttc ttcggtcgtg acccacggac gaaagcactc
     4081 gtcgcggaca tgtccgacgc cgaaatctgg aacctcaagc gcggcggcca cgactaccgc
     4141 aaggtttacg ccgcctaccg tgcggcgaca gagcacaagg gccagccgac ggtgatcctg
     4201 gccaagacca tcaagggcta ctcgctgggc gcgcacttcc agggccgcaa tgcgactcac
     4261 cagatgaaaa agcttgcgct ggaagacctc aagtacttcc gggacgccca acgcattccg
     4321 gtcagtgacg cggagctcga agagaacccg tacttgccgc cgtactacca ccccggcccg
     4381 gaggcccccg agatccgcta cctgctggag cgcaggcggg cgctgggcgg cttcctgccg
     4441 gagcgtcgca ccaagtccaa ggtgctgacg ctgcctggcc gcgacaccta caaggcactc
     4501 aagaagggct caggtaccca agaggtggcc accacgatgg cgaccgtgcg caccttcaaa
     4561 gaactcttgc gggacaaaga aattggcaag cggatcgtgc cgatcatccc ggacgaggcc
     4621 cgtacgttcg gcatggactc ctggttcccg agcctgaaga tctacaaccg caacggccag
     4681 ctctacaccg ccgtggatgc ggaattgatg ttggcgtaca aggaaagtga agtcggacag
     4741 atcctgcacg aagggatcaa cgaggccggt tcgacgtcat cgttcaccgc ggtgggcacc
     4801 tcctacgcca cgcacaacga gccgatgatc ccggtctaca tcttctattc gatgttcggg
     4861 ttccaacgca ccggcgacgg cttctgggcc gcggcagacc agatggcgcg tggcttcgtg
     4921 cttggcgcca ccgccggccg caccacgctg accggtgaag gcctgcagca cgccgacgga
     4981 cactcactgc tgctggcggc gacgaacccc gccgtggtcg cctacgaccc ggcgttcgcc
     5041 tacgagatcg cctacatcgt ggaaagcggc ctgcaccgca tgtacggcga gaacccggag
     5101 aacatctact tctatgtgac gatctacaac gagccgtacg tacagcccgc cgaacccgaa
     5161 ggggtcgacg tcgaagcgct gctgcgcggt atctaccgct accggatggc gccggagaag
     5221 cgcacacacg tcgcgcagat cctggcctcc ggtgtggcga tgccagaagc ggtgcgggcg
     5281 gccgacctcc tggccgagga gtgggacgtc gccgccgacg tgtggtcggt gaccagctgg
     5341 ggtgaactca accgcgacgg cgtggccatc gagaaggaac gactgcgcca tcccgagcgg
     5401 ccggccggca cgccgtttat cactaacgcg ctggccgatg cggcaggccc ggtgatcgcg
     5461 gtgtccgact ggatgcgcgc ggttccggag cagatcaggc cgtgggtgcc gggcacctac
     5521 atcacgctgg gcaccgacgg cttcggcttc tccgacaccc gccctgcggc gcggcgatac
     5581 ttcaataccg atgccgagtc ggtcgtcgtc ggcgtcctcg agggcctcgc gcgcgacggg
     5641 aacatcgatc aatcggtcgc ggtcgaggcg gcgcgcaagt acaggatcga cgacgtgatg
     5701 gccgcccctg agcagacgtc cgacccggga gtcgcctgat cgcaccgcgc agttgtagag
     5761 aactaacagc ttgatcgcgt agcttttagg gatgaccgac aatcggttca tcccgccgaa
     5821 gtccacgctc gaagtgctgg agaccgtccc cgagtcggtg ctgcgccggc tgagcaacta
     5881 ctccggccgg ctggcgaccg aggcagtgcg cgcgatggag gagcggctgc cgtttttcgc
     5941 caacctggag gcgtcgcagc gcgcgagcgt gcaactggtg gtgcagaccg cggtggtcaa
     6001 cttcgtccag tggatgcgcg atccaaggag caatgtcagc tacacggccc aggcgttcga
     6061 ggtggtgcca caagatctgc gccggcgcat cgcgctgcga cagtcggtcg agatggtccg
     6121 ggtcacgatg gagttcttcg aggagtgggt gcccgtgctg gcacggtccg aggaacaagt
     6181 cacggcgatg accgccggaa tcctgcgcta cagccgggat ttggcgttcg cagcggctac
     6241 cgcctacgcc gaccaggccg aggcgcgcgg tgcgtgggac acccggatgg aggccaacgt
     6301 cgtggacgcc gtcgtccgtg gggacaccgg gcccgagctg cagtcgcagg ccgctgcgct
     6361 gaactgggac gccaccgcgc cggccaccgt gatcgtcggg ctgccccaac ccgaccgcat
     6421 cgacctcacc agtgacgacg tccatgacgt cgtcactcgc aacgggcgcg ccgcattatc
     6481 ggacgtccac ggcacctggc tggtggccat cgtctccggc ccgctcgcgc cgaccgaccg
     6541 gtttctcaac gagttgctcg gggtgttcgc cgacgggccg gtggtaatcg ggccgaccgc
     6601 gccgacgttg ggggccgcgc acatcagcgc caccgaggcg atcgctggga tgaacgccgt
     6661 cgcgggttgg agcggggcgc cacgacccgt atcggcgcga gagttgctgc ccgaacgagc
     6721 cctgctaggc gatgcgaccg cggtcgcagc cctggaagcc gaggtaatgc gaccgttggc
     6781 cgacgccggc ccggcgctaa ccgagacgct cgacgcgtat ctggactccg gcggcgcgat
     6841 cgaggcctgc gcccgcaaat tgttcgttca tccaaacacc gtccgttacc ggctgaaacg
     6901 tatcgccgac ttcaccggcc gcgatcccac gctcccgcgc gacgcgtatg tgctgcgggt
     6961 cgccgcaacc gtggggcggc tgggccgtca cgcgtaccac cccagcacgt caaatgcctc
     7021 gtcagaagtg gtcaagtccg accatcagag gccacaggga gtgcatcgcg ccgggtaata
     7081 ggttgcggag cggtatcggt ggagtcaaat cacatgccgt tttgtggagg tcctacaaaa
     7141 acataagacg aggttcataa tctcttacac cgcgcgaaac cgtcttcaca gtgttctctt
     7201 agagacgtgc ttgcattgct tgcgcccgga cagggctccc agactcccgg catgctcgcc
     7261 gagtggctcg agctgcccgg cgctgccgag cgtctcgcag cctggtcgca gatcagcggc
     7321 ctggacctgg tgcgcctggg gaccaccgcg accgcggagg agatcactga cacggctgtg
     7381 acccagccgc tcgtggtggc cgcgacgctg ctggcccacg aggaactgac ccggcgcggg
     7441 tccctcgcgg ataaggaaac ggttgtggcc ggccattcgg tcggcgagat cgctgcgtac
     7501 gcgatcgccg gcgtcatctc cgccgacgac gccgtcaagc tcgcggccac ccgcggtgcg
     7561 gagatggcca aggcctgcgc gctcgagccg acggggatgt cggccgtgct cggcggcgac
     7621 gaagctgagg tgctggcccg cctggaggca ctggatctcg ttccggccaa ccgcaacgct
     7681 gccggtcaga tcgtggccgc cggtgcgctg tccgcactgg acaagctcgc cgaggatccg
     7741 ccggccaagg cccgggtccg ccagctggcc accgcaggcg ctttccacac gcacttcatg
     7801 gcctcggcca ccgacggata tgccagtgcc gttgaaaagg taacggccgc tgagtccttg
     7861 aggcccaccg cgaaactcct gtcgaacgcc gacggccagc ccgtcgcctc ggcggcggac
     7921 gccatgacca agttggtggc gcagctgact cgtccggtgc gttgggactt atgcacggag
     7981 acaatgcgtc aaagcaatgt gacggcgatc gtcgagttcc cgcccgcggg caccctggcg
     8041 ggcatcgcca aacgtgaact tcggggggtg ccgacgcacg ccgtcaagtc ccccgctgag
     8101 ctggacgggc tagctgagct ctaaccagta caaccacata tcgaatcgtc aatccgaaat
     8161 cgtttctccg ctccgcggag ggcatgtaac aacccgatta gaaggagcca ctgtggccac
     8221 cactcaggaa gaaatcatcg ccggtatcgc cgagatcatc gaagaggtca ccggtatcga
     8281 gccgtccgag gtcaccccgg agaagtcgtt cgtcgacgac ctggacatcg actcgctgtc
     8341 gatggtggag atcgcggtgc agaccgagga caagtacggc gtgaagatcc ccgacgagga
     8401 cctcgcgggt ctgcgcaccg tcggtgacgt cgtcgcctac atccagaagc ttgaggaaga
     8461 gaatcccgag gccgccgcgg ccatccgggc gcagatcgcg gagaccaagt gaccaagcct
     8521 tccactgcta acggcggttt ccccagcgtt gtggtaaccg ccgtcacggc gacgacgtcg
     8581 attgcggcgg acatcgagag cacgtggaag ggcctgctcg caggcgagag tggtatccgc
     8641 gtactcgagg acgagttcgt caccaagtgg gacctgccgg tgcgaatcgg cggtcacctc
     8701 gtcgattcgg tcgacgacca catgacgcga ctggacctgc ggcgcatgtc gtacgtccag
     8761 cggatggcca agctgctcgg cggccaggtc tgggagtccg cgggttcacc cgaggtcgac
     8821 ccggaccggt tcgccgtggt gatcggcacc ggtctgggtg gcggcgagaa gatcgtcgaa
     8881 acctacgacg cgatgaacga gggcggcccc cgcaaggtgt cgccgctcgc cgtgcagatg
     8941 atcatgccga acggtgcggc cgcggtggtt gggctgcagc tcggcgcccg ggccggggtc
     9001 atcaccccgg tgtcggcctg ctcgtcgggt tcggaggcca tcgctcacgg ctggcgccag
     9061 atcgtgatgg gtgacgcgga catcgccgtc gtcggcggtg tcgagggcgg aatcgaggcg
     9121 ctgccgatcg cggcgttctc gatgatgcgc gcgatgtcaa ccaataacga ggacccggcc
     9181 ggtgcatcgc ggccgttcga caagaaccgc gacgggttcg tgttcggcga ggcaggcgcg
     9241 ctgctggtca tcgagaccga ggagcacgcc aaggcccgcg gcgccaagcc gctggcccgg
     9301 ctgatgggcg cgggcatcac gtccgacgcg ttccacatgg tcgcccccgc ctccgacggg
     9361 ttgcgggccg gtcacgcgat gaagcgtgcg atggagaccg cgggcctgga ccccaaggac
     9421 atcgaccacg tcaacgcaca cgcgaccgcc accccgatcg gtgacacggc cgaggcgaac
     9481 gccatccggg tcgccggcgt ggagcacgcg ccggtctatg cgccgaagtc ggcgctcggc
     9541 cactccatcg gtgcggtcgg tgcactcgaa tccgtgttga cggtgcttac gctgcgcgac
     9601 ggcgtcattc cgccgacact gaactatgag acccccgatc ccgagatcga tctcgatgtc
     9661 gtcgcgggcg agcctcgata tggcgaatac cagtacgcca tcaacaactc gttcggtttc
     9721 ggtggtcaca atgtcgccct ggcatttggt cgttactgag cgagaggaac catcggagca
     9781 gtaatgggag ggcttgcaac ggggaacggt ttccccaacg tggtcgtcac cggtatcgcg
     9841 atgacgaccg ccttggcaac cgatgcggag agcacgtggc agaagctgct ggacggacag
     9901 agcggtatcc gcaagctcga cgatccgttc gtcgagcagt acgacctgcc cgtccggatc
     9961 ggcgggcatc ttctcgagga cttcgacagc gagctcactc gtattgagct gcgccggctg
    10021 tcgtacctgc agaagatgtc caccgtgctc gggcggcggg cctggcagaa cgcgggctcg
    10081 ccggaggtgg actcccgtcg gttgatggtg tccatcggca cgggcctggg gtcgaccgag
    10141 gagttggtct tcgcgtacga cgacatgcgc gcgaagggta tgcgggcggt gtccccgctg
    10201 gcggtgcaga agtacatgcc caactcaccg gcggccgccg tcggcctgga gcgtggggcg
    10261 aaggccggtg tcattacgcc ggtttcggct tgcgcgtccg gttccgaggg catcgcgcac
    10321 gcgtggcagc agatcgtgct cggcgaggcc gacatcgcgg tctgcggtgg cgtggaatcg
    10381 aggatcgagg cggtgccgat cgcaggattc gcacagatgc gaatcgtgtt gtccaccaac
    10441 aacgatgacc ctgccggtgc atgtcgtccg ttcgacaggg accgcaacgg gttcgtgttc
    10501 ggcgaagcgg gtgcactgct ggtcatcgag accgaggagc acgccaaggc gcgcggcgcg
    10561 aacatcttgg ctcgcgtgat gggcgccagc gtcacatcgg acggctacca catggtggcg
    10621 cccgatccca acggtgagca ggcggggcac gcgatgacgc gggccatcca gctcgcgggt
    10681 cttgcgccca ctgacatcga ccacgtcaac gcgcacgcga ccggcacctc ggtcggcgac
    10741 gtggccgagg ggcacgcgat caataacgcg atgggcggcc accgaccggc ggtctacgcc
    10801 ccgaagtcgg cgctcggcca ctcggtcggc gcggtcggcg cggtcgagtc gatcctgacc
    10861 atattggcgt tgaagaacgg gatcatcccg ccgacgctga acttgaagaa tctcgacccg
    10921 gagatcgatc tggacgtcgt gcacggcggt cctcgacccg gcaactatca atacgcagtt
    10981 aacaattcgt ttggattcgg tgggcacaac gtcgcgctcg ccttcgggaa atattaagga
    11041 agcaattcct cgaatttctt gatggcatac agccgacatc cgacgcacta caggaggttt
    11101 caatgacaat catggccccc gaggtcgtcg gcgaatcgct agacccacgc gatccgctgc
    11161 tgcgcctgag cacgttcttc gacgacggca gcgtcgagct gctgcacgag cgtgaccgct
    11221 cgggcgtgct ggtcgcggcc ggcaccgtca acggcgtgcg caccatcgcc ttctgcaccg
    11281 acggcaccgt catgggcggc gccatgggtg tggagggctg cgcgcacatc gtcaacgcct
    11341 acgacaccgc gatcgaggag cagagcccca tcgtgggtat ctggcactcc ggtggtgcgc
    11401 ggttggccga gggtgtgaag gcgctgcacg cggtcggtct ggtcttcgag gccatgatcc
    11461 gcgcatccgg ctatgtcccg cagatctcgg tggtcgtcgg cttcgccgcc ggcggcgcgg
    11521 cctacgggcc ggccctcacc gacgtcgtcg tgatggcgcc ggagggccgg gtgttcgtca
    11581 ccggacccga catcgtgcgc agtgtcaccg gcgaggacgt cgacatggcg tcgttgggcg
    11641 gtccggacac ccaccacaag aagtcgggtg tgtgccacat cgtcgccgac aacgagctcg
    11701 acgcgtacga gcgcggccgc cggctggtcg gattgttctg ccagcagggc catttcgacc
    11761 gtgacaaagc tgaagcgggt gacaccgatt tgcatgcgct gctgccggat tcggcgcggc
    11821 gcgcctacga cgtccacccg ctcgtcgatg cgctgctcga cgacgacgcc ccgctggagg
    11881 aattccaggc caagtgggcg ccgtccatgg tggtcggcct cggtcggctc tccgggcgca
    11941 cagtcggcgt tttggccaac aatccgctgc ggctgggcgg ttgcctgaac tccgaaagcg
    12001 ccgagaaggc agcacgtttc gtgcggctgt gcgacgcgtt cgggattccg ctcatcgtcg
    12061 tggtcgacgt gcccggttat ctgcccggtg tggatcagga gtggggcggc gtggtccggc
    12121 gcggcgccaa gctgctgcac gcattcggcg aagcaaccgt tccgcgggtc actctggtca
    12181 cccggaagat ctacggcggg gcctacatcg cgatgaactc gcggtccctt aacgcgacga
    12241 aggtgttcgc gtggccggac gccgaggtcg ccgtcatggg cgccaaggcc gcggtcggca
    12301 tcctgcacaa gagggcgctg gccgccgcgg aagaccatga gcgcgaagcg cttcacgacg
    12361 aactggccgc cgagcacgag cggatcgccg gtggcgtcga ctccgccatc gagatcggcg
    12421 ttgtcgacga gaagatcgac ccggcgcaca ctcgcagcaa gctgacgcag gcactggccg
    12481 aggcgccggc gcgccgtggt cggcacaaga acatcccgct gtaacccagc gcggcggata
    12541 atcgatggcg cgcacgaaga acatattcga ctacgcgtca aaggtgttga gcgccaccgc
    12601 gttctgggca tgggtgttag atgccgagaa cgcggatgac gcgactctga atgcaattaa
    12661 tgccgatcta cggagcaagc tcgccctacc gcagcaggcc agactcgtag acgtcgtgct
    12721 tgcaaagaac ccggatgatc caagactgat gccgcacctt ccatccgagg aaggcgagcg
    12781 tgatgaccgc aagcgaattg acattctcgc cacctatcga ctgcccgagc gcggactgct
    12841 cgatctatct ctcgatcgtc aacaaccgac ccatcgtttc gccgttatcg ccaggcgccg
    12901 tggacgaata gcagcggcac agggtgcacg tcgctaaccg accccttgtc gatcacctcg
    12961 agcatgcgat caggttgaca ggggttcgtc gaggccgaat aagtattcgg atactcgatt
    13021 cttggcgatg atcgcaacat ggacaccgaa ggcctgcgac cggctgatct gcgtcgctgg
    13081 cgtcagcacc tggccaatga acgcgccgaa gcgggtgtgt accggcatct ggcccagcgc
    13141 cggaccggcg aggaacgcga catcttgctc acgctggcag acgccgaacg acgccacgag
    13201 cagcattggc tgaacctgct cggcgatcat gtgggcaagc cacggcgcgc tgacctgcga
    13261 acccgcatgc tcagcttcct cgcgcgacac ttcgggtcgc tattcgtgtt ggccctcgca
    13321 caacgagcag aagcccggtc cgcctacgac accgacgtcg acgccacacc cacgatggcc
    13381 gccgatgagc gcattcactt ggaagtcgtg cgtgcactgg ccacccgcgg gcgcaaccaa
    13441 ttgtcgggca gcttccgcgc cgcggtgttc ggcgccaacg atggcctcgt cagcaacctt
    13501 gcactcgtcc tcggcatcag cgcgacgggc gtcaccaacc acacagtgct cgccaccggc
    13561 cttgccggcc tgctcgcagg cgcgctgtcc atgggcgctg gggagtacgt gtcagtgaat
    13621 tcgcaacgcg aactgctgga agcctccacg ccgagccccg aggctggagc cgcgttgccg
    13681 cagctggatg tgaacgccaa cgagctggcg ctggtctatc gggcacgcgg catgagcgcc
    13741 gaggaggcaa ccggtcacgc ggccaaggtg ctgaacaacc ttgcgacagg cggcagccac
    13801 agccgaatcg cccggcccga ccccgaccag cacgaagccg ttggcacggg tcttcgcgcg
    13861 gcgatttcca gcttcagctt cttcgcatca ggagcgc
//
LOCUS       DASRJA010000325        23201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1173516,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000325 DASRJA010000000
VERSION     DASRJA010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 23201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23201
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173516"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFC01_14205"
     CDS             complement(<1..587)
                     /locus_tag="VFC01_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HZN80818.1"
                     /translation="MKLLGRKKDNSDSDNTADEAVSDTETGQRAGGTTAPKGRPTPKR
                     NQGKRGPIAPAPLTAAEARRRRKEIGGPKLSREERKAERLVRRADMAERREKMMAGED
                     AYLLPRDKGPVRRFVRDVVDSRRNVLGLFMPAALGLIFIMLAVPSVQVQRLLSPAMLV
                     LVLIMVIDGFLVGRRVNKLVDAKFPDNTESGWKLG"
     gene            611..1666
                     /locus_tag="VFC01_14210"
     CDS             611..1666
                     /locus_tag="VFC01_14210"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HZN80819.1"
                     /translation="MQVLIAPDCFGDSLTAVEAAESIARGWQRGRPDDELTLAPQSDG
                     GPGFVGVLAGRVGEVHTLRVSGPLTEEVSADWVLDPAAPRTAYIECAQACGLHLLGGP
                     PTVQTALAAHSRGVGQLIAAAVQAGAGRIVVGLGGSSCTDGGRGMIDELGGLEAGRQL
                     LAGVELVAATDVEHPLLGPKGAAAVFGPQKGADPDTVRVLEQRLSDWAVELNAAAGRA
                     VSDEAGAGAAGGIGAGLLALGGRRESGATIIAEHTRLADDVAAAGLVITGEGRFDDQS
                     LHGKVVSALAAGARSHQIPVLVLAGQVTLDEPTLAEAGIAAAFSITDHAGSVQRAIED
                     AANQLEGLAEATAATWG"
     gene            1749..2105
                     /locus_tag="VFC01_14215"
     CDS             1749..2105
                     /locus_tag="VFC01_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZN80820.1"
                     /translation="MTVQDETATSTHGVTLTDAAAAKAKALLDQEGRDDLALRIAVQP
                     GGCAGLRYNLFFDDRSLDGDLTAEFGGVSLTVDRMSAPYVQGATIDFVDTIEKQGFTI
                     DNPNATGSCACGDSFN"
     gene            complement(2107..2736)
                     /locus_tag="VFC01_14220"
     CDS             complement(2107..2736)
                     /locus_tag="VFC01_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80821.1"
                     /translation="MTGPYPPEYGAGPVGPQMYPPQPYPENVPSPYPTMLPPPLPYPK
                     RRWWRVVAAVAAAVVVVAAVVAAIVFASRDDNSSANTALTETSARAAIQEYLDALARG
                     DDEMVARHTLCGLFDAVKEHRSDLALASLSSDAFRKQYNHAEVTSIDKMVLWSPNQAQ
                     VLFTMKVAPAAGSRFDQPPKDEEQAVAQLLAQDNEILVCSYLPRTAGQY"
     gene            2880..3854
                     /locus_tag="VFC01_14225"
     CDS             2880..3854
                     /locus_tag="VFC01_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZN80822.1"
                     /translation="MTIAVTGSIATDHLMRFPGRFSEQLLPDHLQKVSLSFLVDDLVI
                     HRGGVAGNMAYAIGILGGDVALVGAAGKDFDDYRQWLTSHGVDCDNVLISDSAYTARF
                     VCTTDEAMAQIASFYPGAMSEARNIKLADVVSRTGTTDLVIIGANDPDAMFLHTEECR
                     KLGLAFAADPSQQLTRLSGDQIRQLIDGATYLFTNDYEWDLLLQKSGWSEAEVMGQIE
                     MRITTLGEKGVDIVGRDGTFVHVDVVPETHKDDPTGIGDAFRAGFLTGRSAGLSLERS
                     AQFASMVATLVLEAPGPQEWQWDKDAAVKRLSDAYGADAAAEISQAFA"
     gene            complement(3851..5782)
                     /gene="asnB"
                     /locus_tag="VFC01_14230"
     CDS             complement(3851..5782)
                     /gene="asnB"
                     /locus_tag="VFC01_14230"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZN80823.1"
                     /translation="MCGLLALLTHPSADVSAALVDAVSGSSHLMRHRGPDEPGTWSDD
                     RVVLGFNRLSIIDIAHSHQPLRWGPPESPDRYVLVFNGEIYNYLELREELSAEHNAVF
                     GTDGDSEAIIAAYHHWGTDALTRLRGMFAFALWDTVEKELFCARDPFGIKPLFMATGS
                     GGTAVGSEKKCLLDLADEVGIDLGIDDRAVQHYTVLQYVPEPETLHRGIRRLESGSYA
                     RVRPGQPPEVTRYFAPRFTAKPFGAGSEQSRYDEITAVLEDSVAKHMRADVTVGAFLS
                     GGIDSTAIAALAMRHNPRLITFTTGFEREGFSEVDVAVASAEAIGARHVTKVVNQAEF
                     VAALPEIVWYLDEPVADPALVPLFFIAREARKHVKVVLSGEGADELFGGYTIYREPLS
                     LRPFDYLPRPVRRSLGKASKPLPEGMRGKSLLHRGSLTLEERYYGNARSFSDAQLRTV
                     LPGFREEWTHTDITAPVYATSDGWDPVARMQHIDLFTWLRGDILVKADKMTMANSLEL
                     RVPFLDPEVFAVASQLPAEQKITRATTKFALRKALEPIVPAHVLNRPKLGFPVPIRHW
                     LRAGELMDWAYATIAASQAGDHVDLAAVRAMLDEHRSGAGDHSRRLWTVLIFMLWHAI
                     FVEHSVTPQISEPHYPVQL"
     gene            5856..6941
                     /locus_tag="VFC01_14235"
     CDS             5856..6941
                     /locus_tag="VFC01_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZN80824.1"
                     /translation="MRPHDRKAVTDFVGGSNVTPRGLRVLALSVILGGTTVLLSGCSW
                     SEVLGLGWPEGITPEAHLNRELWIGSLIAALVVGVIVWGLIFWSSAFHRHKKGDTELP
                     RQFGYNMPLELVLTVTPFLIISVLFYFTVVVQEKMLHKDPNPEVVIDVTAFQWNWKFG
                     YQKVNFGDGTFTFDGVDNERKAAMESKPEGKDAHGEEIVGAVRGLNPEDRTYLNFDKV
                     ETLGSSTEIPILVLPTGKRIEFQIASADVVHAFWVPEFLFKRDVMPDPEANHSDNVFQ
                     ISEINKTGAFVGRCAELCGTYHSMMNFEVRVVEPNDFKAYVQQRMAGKTNAEALQAIN
                     QPPTAVTTHPFDTRRGEQAGAAPEAIK"
     gene            6951..7370
                     /locus_tag="VFC01_14240"
     CDS             6951..7370
                     /locus_tag="VFC01_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HZN80825.1"
                     /translation="MHIEARLFEFLTAFFILSAIVYGVLTAMFANGGVEWAGVTALVM
                     TSGLTLITGTFFRFVARRLDTRPEDYEDAEISDGAGELGFYSPHSWWPLFIALSASIT
                     AVGVALWLPWLIVAGVCFVLSTAAGLVFEYYTGPEKH"
     gene            7529..8389
                     /locus_tag="VFC01_14245"
     CDS             7529..8389
                     /locus_tag="VFC01_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HZN80826.1"
                     /translation="MSGPNPPGPEGSDVPGQEPGNQPTADQSAPETGETEIHSQAYSA
                     PESEQFTAGPYVPADASLYDYDSYDPATELVEQEQPPRWPWIVGVAAIIAAIALVASV
                     SVLVTRTDTDNLATPATSTTTAPPVQDEITTTTPPPPPPPPPTTTEAPPPPPPPETVT
                     VTQESPPPPPPPAEAPPPPAATNTPAPAPPATTAPAGPRQVTYSVTGTKAPGDIITVT
                     YIDASGRSRTQRNVYIPWSLTVTPISNSEVGSVQASSLFLVSRLNCSITTSDGTVLSS
                     NTNNAAQTSC"
     gene            8389..9078
                     /locus_tag="VFC01_14250"
     CDS             8389..9078
                     /locus_tag="VFC01_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2561 family protein"
                     /protein_id="HZN80827.1"
                     /translation="MPNNTQAAGRSRSHTGLDTGEFDRTDRILIAACAGVWLVALGAG
                     VAATVALVDLGTQHAQSSGESETPWLLYTVIAVSAAVIVGAVPLLLRARRAALEESPP
                     PARATPVRAVPGQQPPVPGAEAPTEKFRVAPTRGGQVATHPGYATREAIIPPPSPLTA
                     AVDQLWLRCAVVIAIAMGVAVVAIGVATYLMAADSNTAAWVFYVLAGLVTVGMAAIPW
                     FYLRELRTLLA"
     gene            complement(9094..10782)
                     /locus_tag="VFC01_14255"
     CDS             complement(9094..10782)
                     /locus_tag="VFC01_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HZN80828.1"
                     /translation="MSPKLPKPNLAEIAAKQGDDIDSRYHPSAGVRRQLNKVFPTHWS
                     FLLGEIALYSFIVLLITGVYLTLFFDPSMAEVTYNGVYQPLRGVQMSKAFASTLDISF
                     EIRGGLFVRQVHHWAALMFAAAIMVHLARIFFTGAFRRPRETNWVIGSLLLILAMFEG
                     YFGYSLPDDLLSGIGLRAALSSITLGIPLVGTWMHWALFGGDFPCGGLGYQCSADGVL
                     LPRMYALHILLIPGIILALIGVHLAMLWFQKHTDFPGPGKTETNVIGVRVMPVFAVKS
                     GAFFALITGILGLMGGLLQINPIWQLGPYKPSQVSAGSQPDFYMMWTEGLARIWPAWE
                     FYPFGHTIPAAFGVAIIMGLVFGLLIAWPFIEAKVTGDRAHHNLLQRPRDAPVRTAVG
                     SAAIAFYMILTLAAMNDVIAWKFHISLNATTWIGRIGMVVLPAIVYYIAYRWAVGLQR
                     SDRAVLEHGIETGIIKRLPHGAYIELHQPLGPVDDHGHPIPLEYQGAAVPKRMNKLGS
                     AGAPGTGSFLTADPISEHDELTEAAHASERKALTTLREYQERNGSSNGETNGHH"
     gene            complement(10779..11984)
                     /locus_tag="VFC01_14260"
     CDS             complement(10779..11984)
                     /locus_tag="VFC01_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HZN80829.1"
                     /translation="MSETSDVKGTDTPGQKGVPGEPSDAELASMSREQLVELGGKLDG
                     VETVFREDRWPVAGTKAEKRAERTVAYWLLLGGFSGLALLLIFLFWPWEYKPLGAEGE
                     FWYQLATPMYGLTFGLSITAIGVGALLYQKKFIPEEISIQERHDGRSPEIHRKTIAAN
                     LTDALETSTLKRRKMIGLSLGIGLGAFGLGTLVAFIGGLIKNPWKPVVPTAEGNKAVL
                     WTSGWTPRFKGETIFLARATGKPGESPFVKMRPEDLDAGGMETVFPWRESDGDGTTVE
                     SHELLGEIAMGVRNPVMLIRIKPLDMPKVVKRKGQESFNFGEFFAFTKVCSHLGCPSS
                     LYEQQTYRILCPCHQSQFDALHFARPIFGPAARALAQLPITIDQDGYLVANGDFIEPV
                     GPAFWERRS"
     gene            complement(11981..12811)
                     /locus_tag="VFC01_14265"
     CDS             complement(11981..12811)
                     /locus_tag="VFC01_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZN80830.1"
                     /translation="MRLLRRGSMTDKSRRRLRRRLSAAVLLLIGLAVAGGLAATLTPT
                     PQVAVADESASALLRTGKQLFDTSCVTCHGANLQGVPDRGPSLIGVGEAAVYFQVSTG
                     RMPAMRGEAQAARKPPIFDESQIDALGAYVQANGGGPMVPRDDNGHIASESLMGSDVA
                     RGGDLFRLNCASCHNFTGKGGALSSGKYAPDLEPAEPAQIYTAMLTGPQNMPKFSDRQ
                     LSPEEKRDIVAYVREATHAPNPGGYGLGGFGPAPEGMAMWIIGMVAAIGVALWIGARA
                     "
     gene            complement(12823..13434)
                     /locus_tag="VFC01_14270"
     CDS             complement(12823..13434)
                     /locus_tag="VFC01_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HZN80831.1"
                     /translation="MTSAVGTSGTAITSRVHSLNRPNMVSVGTIVWLSSELMFFAGLF
                     AMYFTARAQAAGDWPPHPTELNLVQAVPVTLVLIASSFTCQMGVFAAERGDVFGLRRW
                     YVITFLMGLFFVLGQGYEYLHLVDHGTTIPGSAYGSVFYLATGFHGLHVIGGLIAFIF
                     LLIRTTMSKFTPAQATAAIVVSYYWHFVDIVWIALFATIYFVR"
     gene            13484..14560
                     /gene="trpD"
                     /locus_tag="VFC01_14275"
     CDS             13484..14560
                     /gene="trpD"
                     /locus_tag="VFC01_14275"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZN80832.1"
                     /translation="MRHNRRVTSSDSPTWPQVLGRLTTGQALAPGQAAWAMDQIMTGT
                     ATPAQIAGFGVSMKMKRPTAAEVTELADTMLKHARRVPTDAIGNDTVDVVGTGGDGAN
                     TVNLSTMAAIVVAAAGVPVIKHGNRAASSLSGGADTLEALGVRIDLGPEEVARSVAEV
                     GIGFAFAPQFHPSYRHAGVVRREIGVPTVFNLLGPLTNPASPRAGLIGCAWGELAEVM
                     AGVFATRRSSVLVVHGDDGLDELTTTTTSTIWRVQAGTVERLTFDPAAFGFKRADISE
                     LVGGDAESNAAEVRSVLGGAKGPVRDAVVLNAAGAMVAHAGLASDAKWVPAWESGLAR
                     ATEAIDSGAAEKLLARWVGFTQKV"
     gene            complement(14498..16270)
                     /locus_tag="VFC01_14280"
                     /pseudo
     CDS             complement(14498..16270)
                     /locus_tag="VFC01_14280"
                     /inference="COORDINATES: protein motif:HMM:NF005903.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
     gene            16382..16585
                     /locus_tag="VFC01_14285"
     CDS             16382..16585
                     /locus_tag="VFC01_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80833.1"
                     /translation="MGTVKIDCDDCAVRGPGCQDCVVSVLLGVPETLLDDERRALEVL
                     ADVGLAPRLRLVPIHRQGDSGVA"
     gene            16761..17873
                     /gene="ripC"
                     /locus_tag="VFC01_14290"
     CDS             16761..17873
                     /gene="ripC"
                     /locus_tag="VFC01_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase RipC"
                     /protein_id="HZN80834.1"
                     /translation="MTRGFHALRNPVIGAIAGLTLLGSVVAGHVQADPADDALAKLNE
                     LSRQAEQTTEAMHSAQLDLNNKLAVQQAAEEKHVADAAAVEAAKSQLATFQSSVDKLA
                     AAQYMGGRTDGLDAMLTASSPQGLIDQLSVQRVMAAEMSAQMKSYREVGRQAQLAEEA
                     SAKSAAEAKTAAEQAAAVRADLQSKQSKLQVQIAVVKSQYQALTPGQREALAALPPIP
                     APPAPEAAPPDPGILAAPPEGIPPGDVAPPGALPGGGAPEAATVIQAALSRIGSPYSW
                     GGSGPSAFDCSGLVMWSFQQAGISLPHSSQALAQGGQPVSMDQIQPGDLVTYYSDASH
                     VAIYIGDGMMVHASTYGTPVRVAPVDNAPIHNVRRY"
     gene            17842..18687
                     /locus_tag="VFC01_14295"
     CDS             17842..18687
                     /locus_tag="VFC01_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HZN80835.1"
                     /translation="MRRSTTSAATSRRRIGALLVTELICALFLVGRTPSEPVEPVVAA
                     PASLTTPTAASTTETLSDGRTVHLVGLGGVHTASLLARIGAEMDEAAQAVTAFWGPDW
                     PHEVVIVAAGSDAEFGSLAGGGPDIAATTTAERIMFAPGAAAMSDGSLRIVLRHELFH
                     FASRTATAADAPRWLTEGVADFVGRPATPRPPDAAALALGPHASRGLPTDAELDGPDR
                     SRAYDRAWWFSRFVADAYGTDALRALYLRACGPGHPDAVTAVRETLGADLPTVLARWQ
                     HWISG"
     gene            18699..19826
                     /locus_tag="VFC01_14300"
     CDS             18699..19826
                     /locus_tag="VFC01_14300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN80836.1"
                     /translation="MSRVLLVSNDFPPRRGGIQSYLQNLVNHLVAAGTHTLTVYAPKW
                     KGADEFDREANYEVVRHPGTLMLPVPSVAGRMRKLIERHDADTVWFGAAAPLALMAPM
                     ARDAGAGRVVASTHGHEVGWSMLPLARTALRCIGTGTDAVTYISRYTRGRFASAFGPD
                     AALEHVPPGVDTERFAPDGVARAELRARYRLGERPVVVCVSRLVPRKGQDMLIRAMPA
                     IRQRVPGAALVIVGGGPYLTSLRRLAHSFGVAEHVVFTEGVPGDELPAHHALADVFAM
                     PCRTRGGGLDVEGLGIVFLEASATGVPVVAGRSGGAPETVRDGETGVVVDGWDVGAIA
                     ASVSDVLADPDGAARMGAAGRRWVVDNWQWSTQAEKLAELL"
     gene            complement(19829..21631)
                     /locus_tag="VFC01_14305"
     CDS             complement(19829..21631)
                     /locus_tag="VFC01_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZN80837.1"
                     /translation="MRESSVPASFTVGDHDNIVSSVFSHEREDPDHVIFQRLVDGEWT
                     DVTCKEAANQIRSAALGLIAEGVKPGDRVAVFSATRYEWPILDLAVLAAGALTVPIYE
                     TSSAEQVRFVLADSGAVLIFAETDAHADKIEHLEQELPDLRKVLRIDGSGTPALDALA
                     EAGKSVDAKELDDRLAGIKSGDPATLIYTSGTTGRPKGCQLTHANLMYEIRGAKVCFP
                     ALLDKGERMLVFLPLAHVLARAITLACFANKVTLGFTSDIKNLVPTFGVFKPTLVVSV
                     PRVFEKVYNTAEQNAQNDGKGPIFERAANTAIEYSQALDNGGPGLLLRAKHALFDRLV
                     YGKLRAALGGNCRGAISGGAPLGARLGHFYRGVGLTIYEGYGLTETSAAITVNRIDDV
                     KVGSVGKLVPGNSMRLNDDDELLVKGGVVFGGYWNNDEETKAVFSDGWFHTGDLGAID
                     NDGFLTIIGRKKEIIVTAGGKNVAPALLEDRLRAHPLISQAMAVGDQQPFIAALITID
                     PEAFPGWKERNGKDPKATVGDLATDPDLMAEIELAIKEANQAVSKAEAIRKFRILPVD
                     FTEDTGELTPTLKVKRKVVAEKFASDIEALYEKG"
     gene            21721..22110
                     /locus_tag="VFC01_14310"
     CDS             21721..22110
                     /locus_tag="VFC01_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase / dehydrase and lipid
                     transport"
                     /protein_id="HZN80838.1"
                     /translation="MNSIQIADETFVAADPAEVGRAVADPASWRRWWPDLLLTVVEDR
                     GGLGHRWTVAGALTGTMEIWLEPVLDGVVLHYFLHAEPAGVAAWQLAKMNLAKMNHRR
                     RVAGKNMAFEVKRTLEATRPVGVSRLA"
     gene            22151..22588
                     /locus_tag="VFC01_14315"
     CDS             22151..22588
                     /locus_tag="VFC01_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN80839.1"
                     /translation="MADKTAQTIYIDADPSTVMDVIADIGSYPDWVAEYKETEVLEAD
                     AEGYPKTARLVLDATVLKDTMVLSYDWPADRGSVKWSLVSSSLLKSLDGAYRLSPKGS
                     GTDVTYELSVDLIIPMIGLLKRKAERRLTDTALKDLKKRVEAE"
     gene            22581..>23201
                     /locus_tag="VFC01_14320"
     CDS             22581..>23201
                     /locus_tag="VFC01_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HZN80840.1"
                     /translation="MSDLAAPTGPRISLFVGKGGVGKSTLATATAVRDARAGLRVLIV
                     STDQAHSIGDVLGTVVAPTGLREPTRVLADDADAGGGFLDALALDTLALLESRWRGIA
                     GLLSGRFPESDLGDIAPEELSALPGIQEVLGLHEVGELAASGQWDHVVVDCASTADAL
                     RMLTLPATFGLYLERAWPRHRRLSTGADDARAVAIIDLIEGISAGTE"
BASE COUNT         3827 a         7599 c         7912 g         3863 t
ORIGIN      
        1 aagccgagct tccaaccact ttcggtgttg tcggggaact tcgcgtcgac aagtttgttc
       61 acccggcggc caaccaggaa gccgtcgatc accatgatca acaccaggac aagcatggcg
      121 ggggagagca ggcgctgaac ctggaccgac ggcacggcca gcatgatgaa gatcaggccc
      181 agtgccgcgg gcatgaacag cccgagcaca tttcggcgcg agtccaccac gtcgcggacg
      241 aagcggcgca ccgggccctt gtcgcgtggc agcagatagg cgtcctcgcc ggccatcatc
      301 ttctcgcggc gttcggccat gtcggcgcgg cggacgaggc gttcggcctt gcgttcctcg
      361 cgggacagtt tcggcccgcc gatctccttg cggcgccgcc gcgcttcggc cgcggtcagc
      421 ggcgcgggtg cgatcgggcc gcgcttgccc tggttacgtt tcggggtagg cctgcccttg
      481 ggtgcggtag tgccgccggc gcgctggccg gtctcggtgt cggacacggc ctcgtcggcg
      541 gtgttgtccg agtccgagtt gtctttcttt cggcccagca gtttcacgcc tgccagatta
      601 gtctgccggg gtgcaggtgt tgatcgcccc cgactgcttt ggcgacagcc tcaccgcagt
      661 ggaggccgcc gagtcgatcg cgcgcggctg gcagcgcggc cgccccgacg acgagctcac
      721 cctggccccg cagtcagacg gcgggcccgg cttcgtcggc gtgctggccg gccgggtcgg
      781 tgaggtgcac acccttcggg tgagcgggcc gctgaccgag gaggtgtccg cggactgggt
      841 gctcgacccg gccgcgcctc gcacggccta tatcgaatgc gcgcaggcct gtggtctgca
      901 tctgctgggc ggtccgccga ccgtgcagac cgcgctggcc gcgcacagcc ggggtgtggg
      961 acagctgatc gccgccgcgg tgcaggcggg cgcagggcgg atcgtggtcg ggttgggcgg
     1021 cagcagctgc actgacggtg gccgcggcat gatcgacgaa cttggcggac tcgaggcggg
     1081 ccgccagctg ctggcgggtg tcgagctcgt cgccgccacc gacgtggagc acccgctgct
     1141 cggaccgaag ggcgccgcgg ccgtcttcgg cccgcagaaa ggggccgacc ccgacaccgt
     1201 gcgcgtgctg gaacaacggc tcagcgactg ggcggtcgag ttgaacgcgg ccgcagggcg
     1261 cgcggtcagc gacgaagcgg gtgcgggcgc cgcaggaggc atcggcgcgg gactgctggc
     1321 gctgggcggc cgccgcgaat ccggtgccac gatcatcgcc gaacacaccc ggctggccga
     1381 cgacgtggcc gccgccggac tcgtgatcac cggcgagggc cggttcgacg accagtcgct
     1441 gcacggcaaa gtggtcagcg cgctggccgc aggcgcgcgc tcgcaccaga taccggtgtt
     1501 ggtgctcgca ggccaggtca cgctcgacga acccacactg gccgaggcgg gcatcgcggc
     1561 ggcgttctcc atcaccgacc atgcggggtc cgtgcagcgg gccatcgagg acgcggcgaa
     1621 tcagctggaa gggctggccg aggcgacggc ggcgacctgg ggataaatcg ggaatagggc
     1681 gcaaacaagg taccgttgat gaagtggacc ccaagggtct acccacgaac atgcacaggg
     1741 gagatgtaat gactgttcag gacgagacgg ccaccagcac ccacggcgtc accctgaccg
     1801 acgccgcggc cgccaaggcg aaggcgctgc tggatcaaga gggccgtgac gacctggcgt
     1861 tgcgcatcgc cgtccagccg ggcgggtgcg ctggcctgcg ctacaacctc tttttcgatg
     1921 accggtcgct ggacggtgac ctgaccgcag agtttggcgg cgtctcgctg accgtcgacc
     1981 ggatgagcgc cccctatgtg cagggcgcca caatcgactt cgtcgacacc atcgagaagc
     2041 agggctttac catcgacaac ccgaacgcca ccgggtcctg cgcctgcggc gactcgttca
     2101 actgagttag tactggccgg ccgtcctcgg caagtaagag cacaccagga tctcgttgtc
     2161 ctgggccagc agctgcgcga cggcctgttc ctcgtctttg ggcggctggt caaaccttga
     2221 tccggccgcg ggcgcgacct tcatcgtgaa cagcacctgc gcctgattcg gcgaccacag
     2281 caccatcttg tcgatcgagg tgacctcggc gtggttgtac tgcttgcgga acgcgtcgct
     2341 gctcaagctg gccagcgcga ggtcggagcg gtgctccttg acggcgtcga agagtccgca
     2401 caaagtgtgc cgcgcgacca tttcatcgtc gcccctcgcc agcgcgtcca ggtattcctg
     2461 aatcgcggcc cgggctgatg tttctgtgag cgcagtgttg gcagacgaat tatcgtcgcg
     2521 cgacgcgaaa acaattgcgg cgaccactgc tgcgacaacg acgaccgcgg ctgccaccgc
     2581 cgcgacgact cgccaccacc gccgtttcgg atatggcagc ggcggcggca gcatcgtcgg
     2641 gtatggcgac ggcacgtttt cgggataggg ctgcggcgga tacatttgcg ggccgaccgg
     2701 tccagcgccg tattcgggcg ggtacggacc agtcatatat ttgctccccc gagatgtgtc
     2761 gagaatttct gctgtctaca gtgaccgagc gggtacttgt aggcaggtta gcgcacggtc
     2821 atcgagtatc ccgtgggtaa ggttatgtag acggcttaat aaatgaaggg tgacgtttcg
     2881 tgaccattgc ggtgaccggc tcgattgcca ccgaccatct catgcgtttt cccggtcggt
     2941 tctccgagca acttctgccc gatcacctgc agaaggtgtc attgagtttc ctggtcgacg
     3001 atctggtcat ccatcgcggc ggcgtggcgg gcaacatggc gtacgcgatc ggcatactcg
     3061 gcggtgacgt cgccctggtc ggcgctgcgg gcaaggactt tgacgactac cggcagtggc
     3121 tgacgtcgca tggcgtggac tgcgacaacg tcctcatttc cgactcggcg tacacggcgc
     3181 ggttcgtctg cacgactgat gaggccatgg cccagatcgc ctcgttctac ccgggagcta
     3241 tgtcggaggc ccgcaacatc aaactcgctg acgtcgtatc acgaacgggc acaactgatt
     3301 tggtaatcat cggagccaac gaccccgacg cgatgttcct gcacaccgag gagtgtcgca
     3361 agctgggcct ggcgttcgcc gccgacccgt cgcagcaatt gacccggttg tccggcgacc
     3421 aaatccgcca attgatcgat ggtgcgacct acctgttcac caacgactac gaatgggacc
     3481 tgctactgca gaagtccggc tggtccgagg ccgaggtgat gggccagatc gagatgcgca
     3541 tcaccacgtt gggggagaag ggcgtcgaca tcgtcggccg cgacggcact ttcgtccacg
     3601 tcgatgtggt gcccgagaca cacaaggacg acccgaccgg catcggcgac gcattccggg
     3661 cgggcttcct caccggccgc agcgccgggt tgagcctcga gcggtcggcg cagttcgcat
     3721 cgatggtcgc gactctcgtg ctggaggcgc ccggtccgca ggaatggcag tgggacaagg
     3781 atgccgccgt caaacggctg tcggacgcgt acggagcaga cgcggccgcg gagatctcgc
     3841 aggcgtttgc ctagagctgc acggggtagt gcggctcgct gatctgtggc gtcacgctgt
     3901 gctcgacgaa gatcgcgtgc cacagcatga agatcagcac cgtccacagc cggcggctgt
     3961 gatcgcctgc gccgctgcgg tgttcgtcaa gcatggcccg caccgcggca agatccacat
     4021 ggtcgccggc ctgtgaggcg gcgatcgtcg cataggccca gtccatcagt tcacccgccc
     4081 gcaaccaatg ccggatcggg acggggaagc ccagcttcgg ccggttgagc acatgcgcgg
     4141 gaacgatcgg ttccaacgcc ttgcgtaacg cgaatttcgt tgtggccctg gtgatcttct
     4201 gctccgcggg cagctgcgac gccaccgcga acacctcggg atccagaaac gggacccgca
     4261 gttcgagcga attggccatc gtcatcttgt cggccttgac caggatgtcg ccacgcagcc
     4321 aggtgaacag gtcgatgtgt tgcatccgcg cgaccgggtc ccagccgtcg gacgtggcgt
     4381 acaccggcgc ggtgatgtcg gtgtgcgtcc actcctcgcg gaagcccggc agcaccgtcc
     4441 gcagttgggc gtcggagaag ctgcgcgcgt tgccgtagta gcgctcctcg agagtgagcg
     4501 atccgcggtg cagcaggctc ttgccgcgca tgccctccgg cagcggtttg gacgcctttc
     4561 ccaacgaacg tcgaaccggg cggggaagat agtcgaaagg ccgcagcgac aacggttccc
     4621 ggtaaatggt gtaaccgccg aacagttcgt cggcgccctc accggacagc accaccttga
     4681 cgtgcttgcg ggcctcgcgt gcgatgaaga acagcggcac cagcgccggg tccgcgacgg
     4741 gttcatcgag ataccagacg atttcgggta gtgccgcgac gaattcggcc tgattgacca
     4801 ccttcgtcac gtgccgggcg ccgatcgcct cggccgaggc aaccgcgacg tccacctcgg
     4861 agaacccctc ccgttcgaag ccggtggtga acgtgatcag tcgcgggttg tggcgcatcg
     4921 ccaaagcggc gatcgcggtg gagtcgattc cgcccgacag aaaggcgccg acggtcacgt
     4981 cggcgcgcat gtgcttggcg accgaatcct cgagcaccgc agtgatttcg tcgtagcggc
     5041 tctgctcgct gcccgcaccg aacggcttcg cggtgaaccg cggtgcgaag tagcgggtca
     5101 cctcaggtgg ctgtcccggc cgcacgcggg cgtaactgcc cgattccagc cgccggatgc
     5161 cgcggtgcag cgtctccggt tcgggcacgt actgcagcac cgtgtagtgc tgcacggcgc
     5221 gatcgtcgat gccgaggtcg atgccgactt cgtcggccag gtccagcagg cacttcttct
     5281 cgctgccgac cgccgtcccg ccggatcccg tcgccatgaa cagcggcttg atgccgaaag
     5341 ggtcacgcgc gcagaacagt tccttctcga cggtgtccca cagcgcaaat gcgaacatgc
     5401 cgcgcaaccg cgtgagcgcg tcggtacccc agtggtggta ggcggcgatg atcgcctcac
     5461 tgtcgccatc ggtaccgaag acggcgttgt gctcggccga gagctcctcg cgcagctcga
     5521 ggtagttgta gatctcgccg ttgaacacca gcacatagcg gtcaggcgac tcgggcggcc
     5581 cccagcgcaa tggctgatgg ctgtgcgcga tgtcgatgat ggacagccgg ttgaatccca
     5641 atacgacgcg gtcgtcagac caggtgcccg gctcgtccgg gccgcggtgg cgcatgaggt
     5701 gcgaggaacc cgaaacagca tcgaccagcg ccgctgagac gtcagcggac gggtgggtaa
     5761 gcagggccag cagtccgcac accgcgccag tatgcctaat ccgtgaggtg tgtcgagacc
     5821 ggcacctgac ggtggtctac gctgcgtagt attcactgcg tccgcatgac cgcaaggcgg
     5881 tcactgactt cgtaggaggc tccaacgtga caccgcgcgg gcttcgggtg ttggcgttgt
     5941 cagtcatcct gggtggcacg accgtgctgc tcagcgggtg cagctggtcc gaagtgctgg
     6001 gccttggctg gccggagggc atcaccccgg aggcacacct gaaccgcgaa ctgtggatcg
     6061 gttcgctgat cgcggcgctg gtggtcggtg tgatcgtgtg gggcctgatc ttctggagct
     6121 ccgcgtttca ccggcacaag aagggcgaca ccgagctgcc ccgccagttc ggctacaaca
     6181 tgccgctgga gctggtgctc accgtcacgc cgttcctgat catctcggtg ctcttctatt
     6241 tcaccgtggt ggtgcaggag aagatgctgc acaaggaccc caatcccgag gtcgtcatcg
     6301 acgtcaccgc cttccagtgg aactggaagt tcggctacca gaaggtcaac ttcggcgacg
     6361 gcacgttcac cttcgacggc gtcgacaacg agcgcaaggc cgcgatggag tccaagcccg
     6421 agggcaagga cgcgcacggc gaggagatcg tcggtgcggt gcgtggcctc aaccccgagg
     6481 accgcacgta cctgaacttc gacaaggtcg agacgctggg cagctccacc gagattccga
     6541 tcttggtgct gccgaccggc aagcgcatcg agttccagat cgcctccgcc gacgtcgtcc
     6601 atgcgttctg ggtgccggag ttcttgttca agcgcgacgt gatgcccgat ccggaggcca
     6661 accactccga caacgtcttc cagatcagcg agatcaacaa gaccggtgcg ttcgtcggcc
     6721 gatgcgcgga gttgtgcggc acctaccact cgatgatgaa cttcgaggtt cgggtggtgg
     6781 agcccaacga tttcaaggcc tatgtgcaac agcgcatggc gggcaagacc aacgccgagg
     6841 cgcttcaggc aatcaaccag ccgccgacgg cggtcaccac gcatccgttc gacacccgcc
     6901 gtggcgagca ggccggcgcg gcacccgaag ccatcaagta gggacaccac atgcacatcg
     6961 aagccaggct gttcgagttc ctgaccgcgt tcttcatcct ctcggcgatc gtctacggcg
     7021 tgctgaccgc gatgttcgcc aacggcgggg tcgagtgggc cggcgtgacc gcactggtga
     7081 tgacgtccgg gctgaccctg atcaccggca cgttcttccg cttcgtcgcc cgcaggctcg
     7141 acacccggcc cgaggactac gaggacgccg agatcagcga tggcgctggt gagctcggct
     7201 tctacagccc gcacagctgg tggccgttgt tcatcgcgct gtcggcgtcg atcacggccg
     7261 tcggcgtcgc gctgtggctg ccttggctga tcgtggcggg cgtctgcttc gtgctgtcga
     7321 cggccgcagg cctggtattc gagtactaca ccggtccgga aaagcactga ctgggcccac
     7381 accgaaggta ggggagacaa ggtcacaatc gggacaacag ttcgcccgca aaccgccctg
     7441 tgagattcgt tcatcccacc gctttgggta gtgttgccga ggcacggtca acccgcgccg
     7501 gtgaagtagt cgagaaggat aggcgtagat gagcgggccg aatcccccag ggcctgaagg
     7561 gtcggatgtg ccaggtcagg agccgggcaa ccagcccacg gcggaccagt cagcacccga
     7621 aaccggcgaa accgagatcc actcgcaggc gtattccgcg ccggagtccg agcagttcac
     7681 cgccggcccg tatgtgcccg ccgacgcctc gctgtacgac tacgacagct acgacccggc
     7741 caccgaattg gtcgaacagg agcagccgcc gcgctggccc tggattgtcg gtgtggcggc
     7801 catcatcgcc gccattgcgc tcgtcgcatc cgtttcggtg ttggtgacgc ggaccgacac
     7861 cgacaacctc gccacgcccg cgacgagcac caccaccgca ccgccggtgc aggacgagat
     7921 caccacgacg accccgcctc cgccgcctcc gccgccgccg acgaccactg aggcgcctcc
     7981 gccgccacca ccgccggaga ccgtcacggt gacgcaggaa tcgccgcctc cgccgccgcc
     8041 ccccgccgag gcgcctccgc ctccggcggc gaccaacacg ccggctccgg cgccaccggc
     8101 gactacggcg cccgctgggc ctcggcaggt cacgtactcg gtgaccggca cgaaggcgcc
     8161 cggcgacatc atcaccgtca cctacatcga cgcgtctggg cgcagccgca ctcagcgcaa
     8221 cgtgtacatc ccgtggtcgc tgacggtcac gcccatctca aactccgagg tcggctcggt
     8281 gcaggcgtcc agcctgttcc tggtcagccg gctcaactgc tcgatcacga cgagcgacgg
     8341 cacggtgctc tcgtcgaaca ccaacaacgc cgcgcagact agctgctgat gcccaacaac
     8401 acgcaggccg ccggtcgttc gcggtcgcac actggcctgg acaccggtga attcgaccgc
     8461 accgaccgga ttctgattgc ggcgtgcgct ggggtctggt tggtcgcgct cggcgcaggc
     8521 gtcgcagcga cggtggccct cgtcgatctc ggcacccagc acgcgcaatc cagcggggag
     8581 tcagagacgc cgtggctgct gtacacggtg atcgccgtct ccgccgcggt gatcgtcggc
     8641 gctgtgccgc tgttgctgcg ggcgcggcgc gccgcgctgg aagagtcacc gccgcctgcg
     8701 cgtgccaccc cggttcgggc cgtgccaggg cagcagccgc cggttccggg tgccgaggcg
     8761 ccgaccgaga aatttcgagt ggcgccgacg aggggcggtc aggtggccac tcaccccggc
     8821 tacgccacgc gtgaggcgat catcccaccg ccgagcccgt tgacggcggc ggtggaccag
     8881 ctatggctgc gctgcgccgt ggtcatcgcc atcgccatgg gtgtcgcggt ggtggccatc
     8941 ggtgtggcca cttatctgat ggccgccgac agcaacacgg ccgcgtgggt gttctacgtg
     9001 ctcgccggac ttgtcacggt cggcatggcc gctatcccgt ggttctacct gcgcgagctg
     9061 cgcaccctgt tggcctgaga ctgcaaccaa ccgtcagtgg tgcccgttcg tctcgccgtt
     9121 cgacgacccg ttgcgctcct ggtactcgcg taacgtggtg agcgccttgc gttctgaggc
     9181 gtgggcggcc tcggtcagct cgtcgtgttc ggagatcggg tcggcagtta agaagctgcc
     9241 agtgccgggc gcgccggcgg acccgagctt gttcatccgc ttgggcaccg cagcgccctg
     9301 gtactccaac ggaatcggat gcccgtggtc gtcgacaggg ccgagcggtt ggtgcagctc
     9361 aatgtacgcg ccgtgcggca gccgcttgat gatgccggtc tcgatgccgt gctcgagcac
     9421 tgcgcggtcg ctgcgctgca gcccgacggc ccaccggtag gcgatgtagt agacgatcgc
     9481 cggcagcacc accatgccga tgcggccgat ccacgtcgtc gcgttgagcg agatgtggaa
     9541 cttccacgcg atgacgtcgt tcatcgccgc aagcgtcagg atcatgtaga acgcgatcgc
     9601 agcggaacca acggcggtgc gcaccggtgc atctcgcggc cgctgcaaca agttgtgatg
     9661 cgcgcggtcg cccgtcacct tggcctcgat gaacggccac gcgatcagca gcccgaacac
     9721 cagacccatg attattgcga caccgaacgc ggcggggatg gtgtggccga acgggtagaa
     9781 ctcccacgcc ggccagatac gggccaggcc ctcggtccac atcatgtaga agtcgggctg
     9841 gctgcccgcc gagacttggg acggtttgta cggacccagt tgccagatcg ggttgatctg
     9901 cagcagcccg cccatcaggc cgaggatgcc ggtgatgagc gcgaagaacg ctccggactt
     9961 gacggcgaag accggcatga cccgtacgcc aatgacgttc gtctcggtct tgcccggtcc
    10021 cgggaagtcg gtgtgcttct ggaaccacag catcgcgagg tgtacaccga tgagcgccag
    10081 gatgattccg gggatcagca ggatgtgcag tgcgtacatg cggggcagca ggacgccgtc
    10141 cgcggagcac tggtaaccca ggccaccgca tgggaagtcg ccgccgaaca gcgcccagtg
    10201 catccaggtg ccgaccagcg ggatgcccag cgtgatcgac gagagcgccg cgcgcaggcc
    10261 gatgccggac agcaggtcgt cgggcagcga gtagccgaag tagccttcga acatcgccag
    10321 gatcagcagc agcgagccga tcacccagtt ggtttcgcgg ggacggcgga acgcgccggt
    10381 gaagaagatg cgggccaggt gcaccatgat cgcggcggcg aacatcagtg ccgcccagtg
    10441 gtggacctgc cggacgaaca atccgccgcg tatttcgaag ctgatgtcca gcgtggacgc
    10501 gaaggccttg gacatctgga ctccgcgcag cggctgatac acgccgttgt aggtgacctc
    10561 ggccatcgac gggtcgaaga acagcgtcag gtacacacct gtgatcagca ggacgatgaa
    10621 cgaatacagc gcgatctcgc cgagcaggaa cgaccagtgc gtcgggaaca ccttgttcag
    10681 ctgccgacgc acccccgccg acgggtggta ccgcgaatcg atgtcgtcgc cttgcttggc
    10741 agcgatttcg gccagattcg gctttggcag ttttggactc atgagcgccg ctcccagaat
    10801 gccggtccga cgggttcgat gaagtcgccg ttggcgacca ggtacccgtc ttgatcaatg
    10861 gtgatgggca gctgcgccaa agcgcgcgca gccggaccga atatgggcct cgcgaagtgc
    10921 aacgcgtcga actgcgattg gtggcacggg cagaggattc gataggtctg ttgctcgtaa
    10981 agcgaagagg ggcaacccaa gtggctgcac accttggtga acgcgaaaaa ttcgccgaag
    11041 ttgaagctct cctggccctt gcgcttgacc actttgggca tgtcgagcgg cttgatgcga
    11101 atcagcatga cggggttgcg cacacccatc gcgatctcgc ccagcagctc gtgagattcg
    11161 accgtggtgc cgtcgccgtc ggattcccgc cacgggaaca ccgtctccat gccgccggcg
    11221 tcgaggtcct ccggacgcat cttgacgaac ggcgattcgc ccggcttgcc ggtggcgcgg
    11281 gccaggaaga tggtctcgcc cttgaaccgg ggggtccagc ccgacgtcca cagcacggcc
    11341 ttgttgcctt cggcggtcgg aacgacaggc ttccacgggt tcttgatgag cccgccgatg
    11401 aacgcgacca gcgtgccgag gccgaacgcc ccgaggccga taccgagcga caggccgatc
    11461 atcttgcgcc gcttcagagt tgaagtctcc agcgcatcgg tgaggttggc cgcgattgtc
    11521 tttcggtgaa tctcggggga gcggccgtca tgacgctcct ggatcgagat ctcctcgggg
    11581 atgaacttct tttggtacag caatgcgccg acgccgatcg cggtgatcga cagcccgaac
    11641 gtcaggccgt acatcggggt ggccagctgg taccagaact cgccctcggc gcccagcggc
    11701 ttgtattccc acggccagaa caagaagatc agcagcagcg caagccccga aaagccgccc
    11761 agcagaagcc aatacgcgac cgtccgctcg gcccgcttct cggccttggt gccggccacc
    11821 ggccagcggt cctccctgaa gacggtctcg acgccgtcga gcttgccgcc cagctcgacg
    11881 agctgctcgc gggacatcga cgccagttcg gcgtcgctcg gctccccggg cacgcccttc
    11941 tggccgggcg tgtccgtgcc cttgacgtct gacgtttcac tcatgctcgc gccccgatcc
    12001 acagagccac gccgatagcg gcgaccattc cgataatcca catcgccatg ccctccggcg
    12061 cggggccgaa gccgccgagg ccgtagccgc ccgggttcgg cgcgtgggtt gcttcgcgga
    12121 cgtaggccac gatgtcgcgc ttctcctcgg ggctcagctg ccggtcggag aacttcggca
    12181 tgttctgcgg gccggtgagc atcgcggtgt agatctgcgc gggctctgcc ggctccagat
    12241 cgggtgcgta cttgcccgac gacaacgcgc cgcccttgcc ggtgaagttg tggcacgacg
    12301 cgcagttgag ccggaacagg tcgccgccgc gggccacgtc gctacccatc agcgactcgc
    12361 tggcgatatg accgttgtcg tcgcggggaa ccatcggccc gccgccgttg gcctgcacgt
    12421 aggcccccag cgcatcgatc tgcgactcgt cgaagatcgg tggcttgcgt gccgcctgcg
    12481 cctcgccgcg catcgccggc atccggcccg tcgacacctg gaagtagacg gcggcctcgc
    12541 cgacgccgat caggctgggc ccgcggtccg gcacaccctg cagattggcg ccgtggcacg
    12601 tcacgcacga ggtgtcgaac agctgcttgc ctgtgcgcag cagcgctgag gccgactcgt
    12661 cggccactgc gacctgtggc gtcggtgtca gggtggccgc caggccaccg gcgactgcca
    12721 gtccgatcag cagcagcaca gctgccgaca ggcgccggcg cagccgacgg cgggacttgt
    12781 cggtcatcga accccttctc aacagacgca tcggcgagcc gatcaacgga cgaagtagat
    12841 cgtggcgaac aacgcaatcc acacgatgtc gacgaagtgc cagtagtacg agacgacgat
    12901 cgccgcggtg gcctgcgcag gggtgaactt gctcatcgtg gtccggatga gcaggaagat
    12961 gaaggcgatc agaccgccga tgacgtgcag gccgtggaag ccggttgcga ggtagaacac
    13021 cgaaccgtag gcgctgcccg ggatcgtggt gccgtgatcg accaggtgca ggtactcgta
    13081 gccctggccg agcacgaaga acaggcccat caagaacgtg atgacgtacc accggcgaag
    13141 gccgaatacg tcgcctcgct cggcggcgaa aacgcccatc tggcaggtga acgacgacgc
    13201 gatcagcacc agcgtgaccg gcacggcctg aacaaggttc agttccgtcg ggtgcggggg
    13261 ccagtcacct gcggcctggg cgcgcgcggt gaagtacatc gcgaacagcc cagcaaagaa
    13321 catcagctcg ctggacagcc agacaatggt gccgacactg accatattgg gtcggttcag
    13381 cgaatgtact cgcgacgtga ttgcggttcc cgaggtccct acagcgctcg tcacaccgca
    13441 agtatgacgc tttgtagttg ttgaactcca cccgggtcaa gcaattcgtc ataatcggcg
    13501 cgtgacttct tctgactctc cgacgtggcc tcaggtgctc ggccgcttga ccacaggtca
    13561 ggcgctagcc cccggacagg cggcctgggc aatggaccag atcatgaccg gcaccgcgac
    13621 accggcgcag atcgcgggct tcggtgtgtc gatgaaaatg aagcgcccga cggcggccga
    13681 ggtgaccgag ttggccgaca ccatgctcaa gcatgcgcgc cgcgtgccga ccgatgcgat
    13741 cggcaacgac accgtcgacg tggtgggcac aggcggcgac ggagcaaaca ccgtcaacct
    13801 gtcgacgatg gcggcgatcg tcgtcgccgc tgccggggtg ccggtgatca agcacggtaa
    13861 tcgcgcggcg tcgtcgctgt cgggcggtgc cgacacgctc gaggcgctgg gggtgcgcat
    13921 cgacctgggg cccgaagagg tcgcccgcag cgtggccgaa gtcggcatcg gcttcgcatt
    13981 cgccccgcag tttcacccgt cgtatcggca cgccggggtg gtgcgccgtg agatcggggt
    14041 gccgaccgtg ttcaatctgc ttgggccgct gacgaatccg gcttcaccgc gggcggggtt
    14101 gatcggctgc gcctggggtg agctggccga ggtgatggcc ggggtgttcg ccactcggcg
    14161 gtccagtgtg ctcgtcgtgc acggtgacga cggcctcgac gagctgacca ccacgacgac
    14221 gagcaccatc tggcgggttc aggcaggcac cgtagagcgg ctgacgttcg atcccgcggc
    14281 gttcgggttc aagcgcgccg acatctccga gcttgtcggg ggtgacgcgg aatccaatgc
    14341 cgctgaagtc cgttcggtct taggcggggc caagggtccg gtccgcgacg cggtggtgct
    14401 caacgcggcg ggcgcgatgg tggcccacgc gggactagcc agcgacgcca aatgggtgcc
    14461 cgcctgggag tccggcctgg cccgcgccac tgaggcgatc gactccgggg cggccgagaa
    14521 gctgctcgcg cgttgggtgg ggttcactca gaaggtctga atcacaagtc tgctcggccg
    14581 cgagtcgggc ggaccgcgcc gaggctgccc acgctgccca gcgtcccgcc gccgccaccg
    14641 gtgaggccca tccgggttcg gcccgcacga tgcgcacgcc cggccgggtc agccagcgcg
    14701 cgatcagcgc ggtctcctcg accagcgcgc cgcccagcgg cgcgtcggta gctagaacgg
    14761 cttgtgcgcc aacgcaaatg gcgtcgatga ccggcatcgg tggcacgccg cgacgggcac
    14821 tgcctgccgc cgcgagctga ccgtgccgga tcaccgcgag gtgccagcca ccgtcgccgt
    14881 ccgggcgggc ggccaccaac tccgtgaccg tggccagcgc ccgcagccgt tggccgcgcc
    14941 acagcacctc gatggccgcg gcggcgtggt cgcgtaaccg ggcggcggtt tcgtagcggt
    15001 tgcgctcggc cagatcggcg atgtgcgcga gcaccgcggc gagagcgcgg ttgtcgttgc
    15061 cctcgatcaa ctcgacggcg ccggccgaag ccgacgcata ctcggttggg gtgatgccct
    15121 gcggcgcggg acatggcgat agttcgcgct cgggacaccc gtgcagcgcg gagcggctca
    15181 gccgccttgt gcacgtgcgc actcccgtga atcgggccag cagcgcggcg gtctcgaccg
    15241 cgtcggcgcg cgaccggaac ggtccaatcg ctgtgtcgtg cttgggtgtt cgcactgtgg
    15301 agaaccgcgg gaatggttcg tcggtgagca ccacccacca ccagcggtgc ggaaacttcg
    15361 accggcggtt gtagggcggc gcatgcgcgg cgagcagccg cagttcgcgt acgccggcct
    15421 cgaggtcgtg cgcgcactcg acgtagtcga tggcggtcgc cagcgacgcc atttccttga
    15481 tgcgggtgcg cgggtcggcg ccgttgaagt actggcggac ccgcctgcgc agatcgactg
    15541 ccgtgccgat gtacagcact tcggccgacg gcccgcggaa caggtagacg ccggggcgat
    15601 gtggcagcgc gtcggcgagg tgccgcttgc ggcgctgggc cggtgtcaca tcgggcagat
    15661 acgagcgcag gtcggtgtag gtgtgcacac cctgattgcc cacccgctcg atcagcccgt
    15721 gcagcacatc gacggtggca cgagcatcgt cgagcgcgcg gtgcgtcggt gtggtggttg
    15781 cgctgaagag acgagccagc gccgccagcc gaacgctcgg ggcctcgtcg tgggtgagca
    15841 cgcggcgggc cagccggacc gtgcacagga ccggcgcccg cggccaggga atctggaggc
    15901 gttcggcggc tgcgcgcagg aacccgatgt cgaagccggc gttgtgggcg acgagcaccg
    15961 cgccgcgaga gaactccagg aaggcgggca gcaccgagtc gatggtgggc gcgttgtaca
    16021 ccatcgccga agtgataccg gtgagagcga cgatctgtgg cgggatgtcg cgccccggat
    16081 cgaccagggt ggccagctcg cccagcacgg tcccgccgcg cactttgacc gccccgatct
    16141 cggtgatcgc gtcgtggccc tcgccggagg cgcgcccgcc ggtggtttcc aagtcgacca
    16201 cgacgaacgt cgtctcccgc agtgacaggt cagcgagcgg atcgacgtcg gcgaagctca
    16261 gctggcccat gcgctgacgg tatggaacac ctccgacagt tgctggttgc cacgcggacc
    16321 gcagttactt gtcggtggct gtcgataccg tgcgatcaac accgatcgag aggacagcca
    16381 gatgggcacg gtgaagatcg actgcgacga ctgcgcagtg cgcggtccgg ggtgtcagga
    16441 ctgtgttgtg agcgtgttgc tcggcgtgcc cgagacgttg ctcgacgatg aacgccgagc
    16501 gctcgaagtg ctcgctgacg tagggttggc gccgcgactt cggctggtgc caattcaccg
    16561 gcaaggcgat tcaggagtcg cgtaacgaga tcggttgacg cagccgttga taacaatgtc
    16621 tgaggcggct gttaaatttg cgtcttctgt tggacaacgc cgatgccgtt tcgtaaccta
    16681 tctgagacct aagagcagtt cgacgctttc gtcacggcat ctgaaggacg caaatcttga
    16741 ggctcagccg cacgcgcggg attacacgtg gttttcacgc ccttcggaac cccgttattg
    16801 gtgcgatagc tgggttaacg ctgctcggaa gcgtggtcgc tggacatgtg caggccgacc
    16861 ccgcagatga tgctttagca aaactcaatg agttgtctcg gcaggccgaa caaaccactg
    16921 aagcgatgca ttctgcccag ttggatttga acaacaagct ggctgtgcag caggccgccg
    16981 aggaaaagca tgtcgccgac gcagcggcag tagaagccgc caaatcgcaa ttggcaacat
    17041 ttcaatcatc ggtcgataag ttagctgcgg cgcaatatat gggtggcagg accgacggtt
    17101 tggatgcgat gctcaccgcg agctcaccgc agggtctcat cgatcagctc tcggttcagc
    17161 gggtgatggc cgccgagatg tcggcacaga tgaagagtta ccgcgaggtc ggtcggcagg
    17221 cccagctggc cgaggaagcg tccgccaaat cggccgccga agccaagacc gccgccgagc
    17281 aggcggccgc ggtgcgcgcc gacctgcaat ccaagcaaag caagctgcag gtgcagatcg
    17341 cggtggtcaa gtcgcagtac caggcgctga cgccgggcca gcgggaggcc ctggccgccc
    17401 tcccgccgat ccccgccccg cccgcaccgg aggccgcgcc gccggatcct ggcatcctgg
    17461 ccgcgccgcc ggagggcatc ccacctggcg acgtcgcacc gccgggagcc ctcccaggcg
    17521 gtggagctcc ggaagcagcc accgtcatac aggccgcgct gagccggatc ggctcgccgt
    17581 attcgtgggg cggttcgggc ccgagcgcct tcgactgctc cggactggtg atgtggtcgt
    17641 tccagcaggc cggtatctcg ctgccgcatt ccagccaggc actcgcccaa ggcgggcagc
    17701 cggtgtcgat ggaccagatc caaccgggcg atctcgtcac ctattactcc gacgcgtcgc
    17761 atgtggccat ctacatcggt gacgggatga tggtgcacgc gtcgacctat ggcacgccgg
    17821 tgcgagtcgc cccggtcgac aatgcgccga tccacaacgt ccgccgctac tagtcggcgc
    17881 cggatcggtg ccctgctcgt caccgagctg atctgcgcgc tgttccttgt tggacggacg
    17941 ccgtcggaac cggtcgagcc cgtcgtggcc gcgcccgcgt cgctcaccac acccaccgcc
    18001 gcgtcgacca ccgaaacgtt gagcgacggc cgcaccgtcc acctcgtcgg tctgggcgga
    18061 gtgcacacag cttcgctgct ggcccgaatc ggcgccgaga tggacgaggc cgcgcaggcc
    18121 gtcaccgcgt tctggggacc cgactggcca catgaggtcg tcatcgtggc cgccggatcg
    18181 gacgccgagt tcggcagcct cgcgggcggt ggccccgaca tcgcggccac caccaccgcc
    18241 gagcggatca tgttcgcccc gggtgccgct gcgatgagcg atgggtcctt gcgaatcgtg
    18301 ttgcgccacg agcttttcca cttcgcgtcg cgcacagcca ccgccgccga cgcgccgcgc
    18361 tggctcacgg agggcgtcgc cgatttcgtc ggccgcccgg ccaccccgcg gccgccggac
    18421 gccgcagcgt tggccctggg cccacacgca agtaggggat tgccaaccga cgccgagctg
    18481 gacgggcccg atcgttcccg ggcctacgac cgcgcctggt ggttcagccg gttcgtcgcc
    18541 gacgcctacg gcaccgacgc gctgcgggcg ctgtatctac gagcctgcgg tcccggccat
    18601 cccgacgccg tcaccgccgt gcgcgaaacc ctcggcgccg acctgccaac ggtgctcgcc
    18661 aggtggcagc actggatctc cggctagcct gtctggcgat gagccgggtc ctgctggtta
    18721 gcaatgactt tccgccgcgc cgcggcggca tccagtcgta cctgcagaat ctggtcaacc
    18781 atctggtcgc ggccggtacg cacacgctga ccgtctacgc gccgaagtgg aagggcgccg
    18841 acgagttcga ccgggaggcg aattacgagg tggtgcgcca tccggggacg ttgatgctgc
    18901 ccgtgccgtc cgtggccggc cggatgcgaa agctgatcga gcgccacgac gccgataccg
    18961 tctggttcgg cgctgccgcg ccgctggcgt tgatggcacc gatggcccgc gacgcgggtg
    19021 ccggccgcgt cgttgccagc acacacggcc acgaggtcgg ctggtcgatg ctgccgctgg
    19081 cccgaaccgc gttgcggtgc atcggaactg gcaccgatgc ggtcacctac atcagccgct
    19141 acacgcgcgg ccggttcgcc tcggcgttcg gcccggacgc cgcgctggaa cacgtgccgc
    19201 ccggcgtgga caccgagcgg ttcgcgcccg acggggtggc ccgcgccgag ttgcgcgctc
    19261 gataccggct gggggagcgg cccgtggtgg tgtgcgtatc gcggctggtg ccacgcaagg
    19321 gtcaggacat gctgatcagg gccatgcccg cgatccggca gcgggtgccc ggcgccgcgc
    19381 tcgtcatcgt cggtggcggc ccgtacctca cgtcgctgcg gcggctggcg cacagcttcg
    19441 gcgtggccga gcacgtggtg ttcaccgaag gggtgccggg tgacgaactg cccgcccacc
    19501 acgcgctggc cgatgtgttc gcgatgccgt gccgcacccg cggcgggggt cttgacgtcg
    19561 agggtctcgg catcgtcttt ctcgaggcgt ccgcgacggg cgtgccggtt gtagcgggtc
    19621 ggtcgggcgg ggctcccgaa accgtgcgcg acggcgagac gggtgtggtg gtcgacggct
    19681 gggacgttgg cgcgatcgcg gcgtcggtca gcgacgtgct cgccgacccg gacggggccg
    19741 cgcggatggg cgcggcgggt cgacgatggg tcgtcgacaa ctggcagtgg agcacgcagg
    19801 ccgagaaact cgcggagctc ctctaggttc agcccttttc gtagagcgct tcgatgtcgc
    19861 tggcgaactt ctcggcgacg accttgcgct tgaccttcag agtgggggtc agctcgccgg
    19921 tgtcttcggt gaaatcgact ggcaggatgc ggaacttgcg gatcgcctcc gccttcgaca
    19981 ccgcctgatt ggcctccttg atggccagct cgatctcggc catcagatcg ggatcggtgg
    20041 ccagatcgcc gacggtcgcc ttcggatcct taccgttgcg ttctttccag ccggggaacg
    20101 cctcggggtc aatggtgatc agcgcggcga tgaacggttg ttggtcgccg acggccatcg
    20161 cctggctgat cagcggatgc gcccgcagcc ggtcctccag cagcgccggc gcgacgttct
    20221 tgccgccagc ggtgacgatg atctccttct tgcgcccgat gatcgtcagg aagccgtcgt
    20281 tgtcgatggc gccgagatcg ccggtgtgga accacccgtc ggagaagacg gccttggtct
    20341 cctcgtcgtt gttccagtaa cccccgaaca ccaccccgcc cttgacgagc agctcgtcgt
    20401 cgtcgttcag ccgcatgctg ttgccgggca ccagctttcc gaccgagccg acctttacgt
    20461 cgtcgatccg gttcacggtg atcgccgcgc tggtctcggt gagcccgtag ccctcataga
    20521 tggtcagacc gacaccgcgg tagaaatggc cgagccgggc acccagcggc gcgccgcccg
    20581 agatcgcgcc gcggcagttg ccgcccagcg cggcccgcag cttgccgtac accaggcggt
    20641 cgaacagtgc gtgcttggcc cgcagcagca gcccgggacc gccgttgtcc agcgcctggc
    20701 tgtattcgat cgcggtgttg gcggcccgct cgaagatcgg gcccttgccg tcgttttggg
    20761 cgttctgttc ggcggtgttg tagaccttct cgaacacccg tggcaccgac accaccagcg
    20821 tcggcttgaa cacgccgaag gtcggcacca ggttcttgat gtcactggtg aagccgagcg
    20881 tgaccttgtt ggcgaagcat gccaacgtga tggcgcgggc gagcacgtgg gccagcggca
    20941 ggaacaccag catccgctcg cccttgtcca gcagcgccgg gaagcacacc ttggcgccgc
    21001 ggatctcgta catcagattg gcgtgggtga gctggcagcc cttgggcctg ccggtggtgc
    21061 cagaggtgta gatcagcgtc gcggggtcgc ccgatttgat gccggccagc cggtcgtcga
    21121 gttccttggc gtcgacggat ttgcccgcct cggccagcgc atccagcgcc ggcgtcccgc
    21181 tcccgtcgat ccgcagcacc ttgcgcaggt cgggcagctc ctgttcgagg tgctcgatct
    21241 tgtcggcatg cgcgtcggtc tcggcgaaga tcagcacggc cccggagtca gccagcacga
    21301 atcgcacctg ctcggccgaa ctggtctcgt agatcggcac agtgagagcg cccgcggcca
    21361 ggacggccag gtcgaggatc ggccattcgt agcgcgtcgc tgagaacacc gccacccggt
    21421 cgccgggctt gacgccctcg gcgatcaaac cgagtgcggc tgaacggatc tggttagcgg
    21481 cctccttgca cgtgacgtcc gtccattcgc cgtcgacgag gcgttggaaa atcacgtggt
    21541 cgggatcttc gcgttcgtgc gagaacacgg agctgacgat gttgtcgtga tcgcccacgg
    21601 tgaacgaagc gggaacactg gactcacgca cgatatggcc tctcgactag cggctttgcc
    21661 gttgacagcc tagtcggcca ggttgggccg ggttggacgc atatgtgaag cttgtacgcc
    21721 atgaacagca tccagatcgc ggacgagacg ttcgttgccg ccgaccccgc cgaggtgggc
    21781 agggccgttg cggatcccgc gagctggcgg cggtggtggc ccgacttgct gctgacagtg
    21841 gtcgaggacc gcggcggtct cgggcatcgc tggacggtgg caggcgcgct gaccggaacc
    21901 atggagattt ggctggaacc cgtcctcgac ggggtcgtgc ttcactattt cctgcacgct
    21961 gaacccgcgg gagtcgcggc gtggcagctg gccaagatga acctggcgaa gatgaaccat
    22021 cgtcgccggg tggcgggcaa gaacatggca ttcgaggtga aacggacact tgaggcgacg
    22081 cgcccggtcg gcgtttcgcg cctggcctga tacttcggcc ggaccaagca gcagggggta
    22141 gatttttcac gtggcggaca agacggcgca gaccatctac atcgatgccg atccttcgac
    22201 ggtgatggac gtcatcgccg acatcggctc ctatccggac tgggtcgccg agtacaagga
    22261 gaccgaggtg ctcgaggctg acgccgaggg ctacccgaag accgcccgcc tggtgctgga
    22321 cgccaccgtc ctcaaggaca ccatggtgtt gtcctacgac tggcccgccg accgcggatc
    22381 ggtgaagtgg agcctggtgt ccagctcgct gctgaagtcg ttggacggcg catatcggct
    22441 gtcgcccaag ggttccggca ccgatgtcac ctacgaactc tcggtcgacc tgatcattcc
    22501 gatgatcggg ttgttgaaac ggaaggctga acggcgcctt acggataccg cattgaagga
    22561 cctcaagaaa cgagtcgagg ctgagtgatc tcgcagcccc gaccggtccc cggatcagtc
    22621 tcttcgtcgg caagggcggg gtaggtaagt caacgttggc caccgccacc gcggtgcgcg
    22681 acgcgcgcgc cgggctgcgg gtgctgatcg tctccaccga ccaggcgcat tcgatcggcg
    22741 acgtgcttgg caccgtggtc gcgccgacgg gtcttcgtga gcccacccga gtgctggccg
    22801 acgacgccga tgccggcggc ggctttctcg acgcgctggc gctcgacacg ctagccctct
    22861 tggagtcccg atggcggggg atcgcaggct tgctgtccgg acggtttccc gaatcggatc
    22921 tgggagacat tgccccagaa gaactttcgg cgctgcccgg catccaggag gtgctcggcc
    22981 tgcacgaggt cggcgagctt gccgcctcag ggcagtggga ccacgtggtg gtggactgcg
    23041 cgtcgaccgc cgacgcgctg cgcatgctca cgctgcccgc gactttcggt ctgtacctgg
    23101 aacgcgcctg gccgcggcat cggcggctgt ccaccggcgc cgacgacgcc cgtgcggtgg
    23161 cgatcatcga tctgatcgaa ggcatcagcg cggggacgga g
//
LOCUS       DASRJA010000326         4145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_14821,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000326 DASRJA010000000
VERSION     DASRJA010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14821"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VFC01_14325"
     CDS             complement(<1..769)
                     /locus_tag="VFC01_14325"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HZN80841.1"
                     /translation="MTAAVVGLSHSPLIGKNDPEPEALDRVNAAVQVAKAFVADFAPE
                     LVVLYAPDHYNGFFYKEMPPFCIGTEAHSVGDFASQAGPLSVDTDAARTLAEGVLERG
                     VDLTISARMTVDHGFAQPLETLFDGIDRVPVVPIFVNGVATPLCPVRRIRALGTAVGQ
                     AAAELDRRVLFIASGGLSHDPPVPVLDGAPPRVADALIAGHPPTPEQRSRGEERVVEA
                     GRQYAAGSSTMIPINPAWDNRLLDLLERGDLPGFDTWS"
     gene            complement(766..1761)
                     /locus_tag="VFC01_14330"
     CDS             complement(766..1761)
                     /locus_tag="VFC01_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN80842.1"
                     /translation="MLAATAVSQSAADPLSGLVLSELAQPRPKPGWAVVKVVSSSLNM
                     HDLWTLRGVGHPAEQLPIVLGCDAAGYDEDGNAVIVYPVIGDYDAGRGDETLDPNRVL
                     LSERHDGAFAEYLTVPRRNLVPKPDWLSFDEAACLPVAWTTAYRMLFTQANIRAGDRV
                     LVQGAGGGVASAAIKLALAAGAVVYATSRSESKRAQAMGWGARVALPPGERLPERVDL
                     VIETVGEATWSHSLKSLRPGGTVVIAGATTGMNPPADLGRIFYLQQRILGSTGSTRAE
                     LVAMLRMLEATGVRPVIDRTLPLTDIHKGFQLMIDGSLTGKVVVHPAAPDDQGMS"
     gene            2049..3221
                     /locus_tag="VFC01_14335"
     CDS             2049..3221
                     /locus_tag="VFC01_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZN80843.1"
                     /translation="MFASSWLFLAHEDQFHRPGDFFTTYMGEDPIIVTIDKNRRIRAF
                     LNSCRHRGARVCRADYGATRSFTCTYHGWAYDLEGKLVSVPNKDGYPDSFRTEDWGLV
                     EVPHVASYRGLIFGNWDPEPVSLRDSLGDIAWYLDAMLDHDADGTIVVGGVHKWVLEG
                     NWKLAAEQFASDWYHVNMSHASALTVLSPDGKPKDEVVHRTGRQYADPKGHGHGFPVH
                     PRNRFDAQTVHEWMDYAALRERLGDARVEGPLTTGHGNVFPNFSYLPVNGSIRVWHPK
                     GPDRMEVWAWTIVDKSMPEHVREAQRVYNLRTFGPSGIFEQDDGENWSEVQAISHGFI
                     TNSVPLNYQMGIGSERRDDRHPGITSELYSDAAARYFYARWRHLMNTPAWHERKTS"
     gene            3218..3553
                     /locus_tag="VFC01_14340"
     CDS             3218..3553
                     /locus_tag="VFC01_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HZN80844.1"
                     /translation="MSEPIRVAAVDDIPQGEGIAIDKTITGTADDIALLRDDDGSVWA
                     LNDTCTHAEASLAEGWVEDGFVECPLHSSKFCLKTGKVQGLPATCDAVAHRVEVRDGD
                     VFLFPQAGQ"
     gene            3556..>4145
                     /locus_tag="VFC01_14345"
     CDS             3556..>4145
                     /locus_tag="VFC01_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN80845.1"
                     /translation="MHSVVIVGGGIAGVSTAAALRAGGFDGDVTLVDAGEFPYDRPPL
                     SKDYLAGRVDLKSIALQLSQWYDDHRVRLISQTSVDELRTDLGAVELSDGTTLPADKV
                     VLATGGRAARPPVDGVTNPGIHVLRTADDADRLRVALASRARILIVGAGLIGAEVAST
                     ARELGCEVVIADPVAAPLASAFSPDVATWLHSMHER"
BASE COUNT          773 a         1314 c         1341 g          717 t
ORIGIN      
        1 cgctccaggt atcaaacccg ggcagatcac cacgctcgag caggtcgaga aggcggttgt
       61 cccacgccgg gttgatcgga atcatcgttg atgagccggc ggcgtattgt cgccctgctt
      121 cgaccacgcg ttcttcaccc ctgctgcgct gctcgggcgt cggcggatgg ccggcgatca
      181 gcgcatcggc gactcggggc ggtgctccgt cgagaaccgg caccggcgga tcatgcgaca
      241 ggccaccaga agcgatgaac aacacccgcc ggtcgagctc agcagccgcc tggcccaccg
      301 cggtgccgag cgcgcgaatg cgacggaccg gacacagggg agtggccacc ccgttgacga
      361 agatgggcac aaccgggacg cggtcgatgc cgtcaaacag cgtttccaac ggctgggcga
      421 atccatggtc gacggtcatg cgcgccgaga ttgtcaggtc cacgccccgc tccaggacgc
      481 cttcggccaa tgttctggcg gcgtcggtgt cgaccgacag cggacctgcc tgcgacgcga
      541 agtccccaac cgaatgcgct tctgtgccaa tgcaaaaggg cggcatctcc ttgtagaaga
      601 aaccgttgta gtgatcgggg gcatagagca ccaccaactc aggtgcgaag tcggcgacga
      661 atgccttcgc gacctgcacc gccgcgttga cacggtcaag cgcttccggt tccggatcgt
      721 tcttgccgat gagcggggaa tgagataatc cgacaactgc tgcggtcatg acattccttg
      781 atcgtctggt gcggctgggt gtacgacgac cttgcccgtg agcgagccgt cgatcatgag
      841 ctgaaaaccc ttgtggatgt cggtcagggg caacgtgcgg tcgatgaccg gccggacccc
      901 ggtcgcttcg agcatgcgca gcatggccac aagttcggcc cgggtgcttc cggtggagcc
      961 aagaatccgc tgctgcaagt agaagattcg gcctaggtcg gcgggtgggt tcatcccggt
     1021 ggtcgctccc gcgatcacga ccgtccctcc gggacgcaaa gacttcagcg agtgcgacca
     1081 ggtcgcctcg ccaaccgttt caatgacgag gtcgacgcgt tcggggaggc gttcaccagg
     1141 aggcagtgcg acccgtgcgc cccagcccat cgcttgtgca cgtttggatt cgctgcgact
     1201 ggtcgcgtac acgacagcgc ctgcagcgag agccagcttg atcgccgcgg aggccacacc
     1261 gccgcccgcg ccctggacca gcacgcggtc gccggctctg atgttggctt gggtgaacag
     1321 cattcggtaa gcggtcgtcc atgccaccgg aagacaggcc gcctcgtcga acgacaacca
     1381 gtccggtttg ggcacgagat tgcgacgcgg aaccgtcagg tactcggcga aggcaccgtc
     1441 gtggcgctct gacaacagca cacggttggg atccagtgtt tcatcgccgc ggcctgcgtc
     1501 atagtcaccg atcaccgggt acacgatgac cgcgttgccg tcctcgtcgt aacccgcggc
     1561 atcgcagccc agcacgatcg gcagctgctc ggccgggtgc ccgacaccac gcaacgtcca
     1621 gaggtcatgc atgttcagcg aggacgaaac aaccttgacc acagcccaac ccggcttggg
     1681 gcgaggctgg gccagctcgc ttagcaccag accagacaat gggtcagcgg cgctctgtga
     1741 gacggcggtt gcggcgagca cgtcaccacc atcgcgccgc ccaccctgaa accccagttg
     1801 gtgtgccgct gtgcgaaacg cgctatagag gttgaccggg cgcgtgcagt ttcgtatgtg
     1861 acatgacgtc aacgatcgac ccgggccacg acgcccgaac ggtcagcagc ccgccgtgcg
     1921 gtgcgctgcg gcgacccgcg gaattgcacc gcgacatcga cgtgtccggc ttggtggatt
     1981 ccgacggcgg gctgctggat cgcgccgtgt tcaccgatcc gcagatctac cggcaggagc
     2041 tacgccgcat ctttgccagc agctggctat tcctcgcaca tgaggatcaa ttccaccgcc
     2101 ccggcgactt cttcaccaca tatatgggtg aggacccgat catcgtcacg atcgacaaga
     2161 accggcgcat ccgggccttc ttgaattcat gccggcaccg cggcgcgcgg gtctgccggg
     2221 cggattacgg cgctactcgc agcttcacct gcacctacca cggctgggcg tatgacctgg
     2281 agggaaagct cgtcagtgtg ccgaacaagg acggctatcc cgatagtttc cgcaccgagg
     2341 attggggcct tgtcgaagta ccgcacgtgg caagctatcg cggattgatc ttcggtaact
     2401 gggatcccga gccggtctcg ctacgagact cgttgggcga catcgcgtgg tatctggacg
     2461 cgatgctcga ccacgacgcg gacggcacga tagtggtcgg cggtgtgcac aagtgggtgc
     2521 tcgaaggcaa ctggaagcta gccgccgagc aattcgccag cgattggtat cacgtcaaca
     2581 tgagccacgc ctcggcgctg acggtgctgt cgcctgacgg caagccgaaa gacgaggtgg
     2641 tccaccgcac cgggcggcag tacgccgacc cgaagggtca cgggcatggt tttcccgtgc
     2701 atcccaggaa tcgattcgac gcgcagaccg tgcatgagtg gatggactat gccgctctgc
     2761 gagaacgcct cggcgacgcg cgggtcgagg gcccgttgac caccggccac ggcaacgtct
     2821 tcccgaactt ctcctacctt ccggtcaacg gatcgatacg ggtgtggcat ccgaaaggcc
     2881 ccgaccggat ggaggtctgg gcttggacga ttgtggacaa gtcgatgccc gagcatgtcc
     2941 gcgaggcaca gcgcgtgtac aacctgcgca cgttcggtcc cagcggcatc ttcgaacaag
     3001 acgacggcga gaactggagt gaagtccaag cgatttcaca tggcttcatc accaattccg
     3061 tgccgctgaa ctaccagatg ggtatcggca gtgagcgccg tgacgaccgg caccccggca
     3121 tcaccagtga gttgtacagc gacgcggccg cgcgctactt ctacgcacgc tggcgtcact
     3181 tgatgaacac gccggcctgg catgagcgga aaacatcatg agcgagccaa tccgcgtcgc
     3241 cgcagtggac gacatcccgc aaggggaagg tatcgcgatc gacaaaacga tcaccggtac
     3301 cgccgatgac atcgcactgc tacgtgacga cgacggcagt gtgtgggcct tgaacgacac
     3361 ctgcactcac gcagaggcgt cgctcgccga gggctgggtc gaggacggtt ttgtcgagtg
     3421 cccgcttcac tccagcaagt tctgcctgaa aacgggtaag gtgcaaggcc ttccggccac
     3481 ttgtgatgcg gtggcgcacc gcgtcgaggt acgcgatggc gacgtcttcc tcttcccgca
     3541 ggcggggcaa tgacggtgca cagtgtcgtg atcgtcggcg gaggcatcgc cggcgtcagc
     3601 accgccgctg cgctgcgggc gggtgggttt gatggtgatg tgacattggt cgacgccggt
     3661 gagttcccat atgaccgccc cccgttgtcg aaggactacc tcgccggtcg tgtcgacctc
     3721 aaaagcattg cgctgcagtt gtcgcagtgg tacgacgacc accgtgtgcg actgatctct
     3781 cagaccagtg ttgacgaact gagaacagat ctcggggccg tcgagctgag cgacggaacc
     3841 acgctgcccg cagataaggt cgttctggct accggtggca gggccgctcg gccgccggtc
     3901 gacggcgtca cgaaccccgg gatacacgtg ctgcgcaccg ccgacgacgc cgatcggctc
     3961 agggtcgcat tggcgtcgcg cgcgaggatc ctcatcgtcg gagcggggct catcggagcc
     4021 gaagttgcct ccaccgcccg cgagctgggt tgtgaggtgg tcatcgcgga tcctgttgca
     4081 gccccgctgg cgtcggcgtt ctctcccgat gtcgccacct ggttgcacag catgcatgaa
     4141 aggca
//
LOCUS       DASRJA010000327         3816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2680009,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000327 DASRJA010000000
VERSION     DASRJA010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2680009"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..1381)
                     /locus_tag="VFC01_14350"
     CDS             complement(257..1381)
                     /locus_tag="VFC01_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN80846.1"
                     /translation="MNLPRVVIAGLGDSGVLTAIRLARHAEVVGISAKPALVSGQELG
                     WRLSRPHEWARANWIPFDRFRGLDRVRTVHGTLSGLDLSAGTVSVRHDDGSTSAEGYD
                     ALIISTGVTNGFWRQPNFQSDAEIGADLRAAHDRLTTAGSVIVVGGGAAAISSAANVA
                     RTWPGKRVDLYFPKDRGLVDHHPRAWERVQHRLGEAGVVLHPGHRAVIPDGFGCDQIT
                     SEPVRWSTGQPPASADAVLWAIGRVRPNTDWLPAELLDENGFVRVTPELQVPGRRGVF
                     AVGDVAATDPLRTSARNRADGLLAHNVRAEFAGRPLRSYRPPKRRWGSVLGPQRDGLE
                     VFAPNGRAFTFPAWSFDRVLMPLIVRWGIYRGVRDNHPAL"
     gene            complement(1448..1615)
                     /locus_tag="VFC01_14355"
     CDS             complement(1448..1615)
                     /locus_tag="VFC01_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80847.1"
                     /translation="MKKFGFASIVASGLAAAVLGLAGPAQADVIHHDWIYDIQPQVSV
                     PHVDTSVHQSR"
     gene            1754..3019
                     /locus_tag="VFC01_14360"
     CDS             1754..3019
                     /locus_tag="VFC01_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HZN80848.1"
                     /translation="MQHRRRDPSAGPLLVEDDGALLRARGIRYGRAARFAAPQPIAPW
                     SDPLDAGARGPACPQLPSRLEWVTGPAIEGLALSEDCQVLSVTAPGDADGLPVMVWFH
                     GGAYVSGSGESPQHDADQLALQGRVVVVNVSYRLGVLGYLSPSGVDNLGLRDQILALR
                     WVQNNIGAFGGDPDQVTVFGQSAGADSVYSLMLCEETRGLFRRAIMQSAPLGIRDGRD
                     AMTAAMRTAASADSASELLDAQTAAVAEAARFGLIGRMPFGPIMGLDPLPAASDGDER
                     LADAARRIELLVGYTRNDGGPFAAMDPRIARLKRLGPLGTGVERAAAAALTRRVFAAP
                     ARRLAKAWRRYGGRAATFRVDWAPSRMGACHCIELPLLFDAAAWADAPMLGGAPVDER
                     LAETMRRNWSGFAHRGVDGLDSANQRFRV"
     gene            complement(3009..>3816)
                     /locus_tag="VFC01_14365"
     CDS             complement(3009..>3816)
                     /locus_tag="VFC01_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HZN80849.1"
                     /translation="ERASGGHTVRGFPAFVDGDDAADVRVFATKAEQDAAMGPGIQRL
                     LRLTVASPAKSIERQLNPRTRLVLGANPDGSLSALLDDCADAAVTVLAPNPVWSRTEF
                     ASLSQRVSDALTATTLDIVGRVEKVLAAAHEVQVALPAAPSATQADAIADIRAQLDLL
                     LPSGFVTFTGASHLGDLTRYLTAVGRRLERLPRDVAGDRDRMQRVHAVQDAYDELRQA
                     LSPIRAAAADVRDIGRMIEELRVSLWAQQLGTARPVSEQRIYRAIDAVTP"
BASE COUNT          581 a         1279 c         1316 g          640 t
ORIGIN      
        1 gtggtggacg ctatcggcct gggcggcgcc tgcgaagccg agggccgcgg cggtcattgc
       61 actggcgacg atggtggcaa atccgaactt cttcattttg gtgccttctt ctctgtctct
      121 gcgtcggcct ctcttgccga cctgactgct taaacacaga ggtcagagca ttaattccga
      181 tggcagaaat tgatcggcgg ttggcaggaa tcaatctgtt gccgtgtgct gtgggccaca
      241 ctccggcaac ttcgggctac agcgccgggt ggttgtcgcg cacccctcga tagatacccc
      301 agcggacgat gagcggcatc agcacccggt cgaacgacca tgccgggaac gtgaacgcgc
      361 ggccgttcgg cgcgaacacc tcgagcccgt ctcgctgcgg gccgagcacc gaaccccagc
      421 gccgcttcgg cggccgatag ctgcgtagcg ggcgtcccgc gaactcggca cgcacattgt
      481 gggccagcag accgtcggct cggttgcggg ccgaggttcg cagcgggtcg gtggccgcga
      541 cgtcgccgac tgcgaacacc ccacggcggc cgggaacttg gagttcggga gtcacccgga
      601 cgaagccgtt ctcgtcgagt agctcggcgg gcagccagtc ggtgttgggt cgcacccggc
      661 cgatcgccca gagcacggcg tcggccgaag ccggcggttg gccggtgctc caccggacgg
      721 gctcgctggt gatctgatcg cagccgaaac cgtcgggtat caccgcgcga tgccccgggt
      781 gcagcaccac cccggcctca ccgagtcggt gctggacgcg ctcccacgcc cgcgggtgat
      841 ggtcgacgag gccgcggtct ttcgggaagt agaggtcgac ccgcttaccg ggccaggtcc
      901 ttgccacatt ggccgcgctg ctgatggcgg ccgcgccgcc gccgacgacg atcaccgaac
      961 cggctgtggt caggcgatcg tgggcggcgc gcaggtctgc gccgatttcc gcgtctgact
     1021 ggaagttcgg ctggcgccag aaaccgttgg tcacgcccgt cgagatgatc aacgcgtcgt
     1081 acccctcggc cgaagtcgat ccgtcgtcgt gccgaaccga gacggtgccg gcggagagat
     1141 cgagcccgga cagcgtgccg tggacggtgc gtacccggtc caggccgcgg aacctgtcga
     1201 acgggatcca attggcgcgc gcccattcgt gcggacggga cagccgccag cccagctcct
     1261 gtccgctgac caacgcgggc ttcgccgaga ttccgacgac ctcggcgtgg cgggccagcc
     1321 ggatcgcggt gagcacaccg ctgtcaccca ggccggcgat cacgacgcgg ggcagattca
     1381 tgacgacacg ctgacatacg aagaggggca cccgctatgg ggtgcccctc ttgttctgag
     1441 tcgttggtca gcggctctgg tgaacgctgg tgtcgacgtg cggcacgctc acctgaggct
     1501 ggatgtcgta gatccagtcg tggtggatga catccgcctg cgcggggccg gcgaggccga
     1561 ggaccgctgc cgccaagccg ctggccacaa tgctcgcaaa tccgaacttc ttcatttcga
     1621 tgccttcttc tgtagtctcc gtgtcggccc gtttataact ctattggccg atctaaccgg
     1681 ttaaacacag aggtcagcgg aatcattccg gtggcagcaa ttgatcgccg gttggcagga
     1741 ataccgtgaa tgcgtgcagc atcgacgccg agacccgtcg gctggcccgc tcttggtcga
     1801 ggacgacggc gccctgcttc gtgcccgcgg gattcgctat ggccgagccg cccgcttcgc
     1861 cgcgccgcag cccatcgcgc cgtggtccga ccccctcgac gccggcgcac gcggcccggc
     1921 ctgcccacag ctgccgtcac ggttggagtg ggtcaccggc ccagccatcg agggcctcgc
     1981 gctgagcgag gactgccagg tcctgtcggt gacggcgccc ggtgatgcgg acgggttacc
     2041 agtcatggtc tggtttcacg gcggcgcgta cgtgtcaggc agcggtgaat cgccacagca
     2101 cgacgccgac cagctcgcgc tccagggccg cgtcgtggtg gtcaatgtca gctaccggct
     2161 cggcgtgctc ggctacctca gccccagcgg cgtcgataat cttggtcttc gcgatcagat
     2221 cctggcgctg cgctgggtgc agaacaacat cggtgcgttc ggcggcgatc cggaccaggt
     2281 gaccgtgttc ggccagtccg ccggtgcgga ttcggtgtac tccctgatgc tgtgcgagga
     2341 gacgagaggg ctgttccggc gcgccatcat gcagagcgct ccgctcggga ttcgcgacgg
     2401 ccgcgacgcg atgaccgccg cgatgcggac ggcggcgagc gccgactcgg cgagcgaact
     2461 gttggatgcc cagactgccg ccgtggcgga agccgcgcgg ttcggcctga tcggccgcat
     2521 gccgttcggc ccgatcatgg gattggatcc gcttccggcc gcatcggatg gtgacgagcg
     2581 cttggctgac gcggctcggc gtatcgagtt gctcgtcggt tacacccgca acgacggcgg
     2641 cccgttcgcc gcgatggatc cgcgcatcgc acggctgaaa cgcctcggcc cgctcggcac
     2701 aggcgtcgaa cgcgctgccg ctgcggccct gacgcggcga gtgttcgccg cgcccgcccg
     2761 acgattggcc aaggcgtggc gcaggtatgg cggacgtgcc gcgacatttc gggtggattg
     2821 ggcgccctcg cggatgggtg cctgccactg catcgaactt ccgctgcttt tcgacgcggc
     2881 ggcgtgggcc gatgcgccga tgctcggcgg cgccccggtc gacgagcgcc tggccgagac
     2941 gatgcgccgc aactggagcg gctttgctca ccgcggcgtt gacggactcg attcggccaa
     3001 ccagcgtttt agggtgtgac ggcgtcgatc gcccggtaga tgcgctgctc gctgaccggc
     3061 cgcgcggtgc cgagctgctg tgcccacagg ctcactcgca gctcttcgat catccggcca
     3121 atgtcgcgga catcggcggc ggccgcacga atcggcgaaa gagcttgccg cagttcgtca
     3181 tacgcgtcct gcacggcatg cacacgctgc atgcggtcac gatcgcccgc aacgtcgcgc
     3241 ggcaaccgtt cgaggcggcg gccgaccgcg gtgaggtagc gggtgaggtc gccgagatgc
     3301 gatgcgccgg tgaacgttac gaaaccgctc ggaagcagca ggtcgagttg tgcgcggatg
     3361 tcggcgatcg cgtcggcttg ggtcgcagac ggcgcagcgg gcaacgcgac ctgaacctcg
     3421 tgcgcagcgg caagcacctt ctcgacgcgc cctacgatat ccagcgtcgt cgcggtcaac
     3481 gcgtcggaca ctcgctgcga tagtgacgcg aactcggtgc ggctccagac cgggttcggg
     3541 gccaacaccg tgaccgcggc gtcagcgcag tcgtcgagca gcgcggaaag cgagccatcc
     3601 ggattcgcgc ccagcaccag gcgagtgcgc ggattcagct ggcgttcaat gcttttagcc
     3661 ggcgacgcca ccgtcagccg caacaaccgc tggatgccgg gtcccatcgc ggcgtcttgc
     3721 tcggccttcg tcgcgaacac ccgcacatcc gccgcatcgt ccccgtcgac aaatgccggg
     3781 aatccccgca cggtgtgtcc gccgctggcc cgttcg
//
LOCUS       DASRJA010000328        19141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1718043,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000328 DASRJA010000000
VERSION     DASRJA010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 19141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19141
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718043"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="VFC01_14370"
     CDS             complement(<1..1387)
                     /locus_tag="VFC01_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZN80850.1"
                     /translation="MSRPIADRIRAILALDPMAPAIEYQGAWTDWQALSDIAFGVESH
                     LIAAGLGAGSPVGLVLRNHPAMVAAMLGVLLADSCVVTINPSRGESGLAADISELMLP
                     ALIGVDADWRRPGIAAAPTGSLGLEVGAIPAAVATVPGLERVGPGPFRQEPDGVAVEM
                     LTSGTTGPPKRIPLSYDAFEHTVAAAGAHYGSSKPADLRLRSGVAIVSSPLVHMSGLF
                     RTLLNICEGRKIALLERFQVPEFVDLVVRHRPRAVSLVPSALTMVLDATVPTEVFSSV
                     EVVTSGTAPLPVSVQEAFEERYGVAVLPSYGATEFAGGVAGWNLALHREWAKAKRGSV
                     GRPQSGREIRIVSAENGAEVPTGAQGRIEVRTRGGEWVRTTDLGRVDADGFLFVDGRT
                     DDVIIRGGFKVTPADIVSVLRGHPAVRDAGVTGIPDERLGSVPVGAVELVDGAHVDPG
                     ELLDYARGRLTR"
     gene            complement(1384..2457)
                     /locus_tag="VFC01_14375"
     CDS             complement(1384..2457)
                     /locus_tag="VFC01_14375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN80851.1"
                     /translation="MRFGVFILGDKPNHLSHEEVFANVLEEARWAEELGYDEIWLAEH
                     HFSPYGTLADLPLVAAAIAAQTERVRIGTACMVAPFHDPIHLAERIAMVDNLSGGRFD
                     AGFGRGYQAHEFKGFGIPMDEATARYQECVEIVNLLLSQENVSYEGKYWQLEDVTIHP
                     RPVQDPIPVWGTVMKTPSSFEWLADKGFGAIIGNPYQVDPDLQGALDIYLETQAKKGL
                     AAATGNVWALLNAFCHPDDGFARTYPRDSVELSIQTHRQYSNPFERGGDIPADYKAYS
                     DWFDKHDKQSYEQVLNSHLTLMGDPDRIIEKMRTVIDMGWRNIMLRMSRGGAMDRGKV
                     YESMKLFAQEVIPAATELAAAPA"
     gene            2555..3394
                     /locus_tag="VFC01_14380"
     CDS             2555..3394
                     /locus_tag="VFC01_14380"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZN80852.1"
                     /translation="MTAPVTLSDAVVGTALAPTTYRWTETEVILYALGVGARPPAELR
                     LLDEHCGPAVLPTFALIANWWAVKDLRAALSLGSFPIVHSAQSLRLERPVGASGEVSV
                     TAEVTAVWDKGKHAAIEVTSVGVDDDGPLFSASSQTMVLGGGGFGGDRGPSAAEHPQG
                     APDAVYEDAVRPEQAAIYRLSGDRNPLHIDPAAARTFGFDDVFLHGLCTLGFAARALI
                     NVVGDGDPQTLTSISCRFAKPVMLDSELCTEIWRDGDEVRFQTLQGDVVSLSSGTATL
                     AGC"
     gene            3397..4020
                     /locus_tag="VFC01_14385"
     CDS             3397..4020
                     /locus_tag="VFC01_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN80853.1"
                     /translation="MDQRRARGQRNREALIAAAIELFTANGYEQTTVEQISEAAGVAP
                     RTFFHHFAAKDDILFHGYADRLEEATRRFRASRSGSLWDALAEASEAVATAISETPDI
                     FLVRATMYGSLPALRATMLRINDDWIDQMTTEVARWLDTDAEVDVRPRLAATVINGAN
                     RAAIDLWVAGGGKGDLIAMMAEAVKLMRPSINSIEREAKQARGRRVG"
     gene            4010..4759
                     /locus_tag="VFC01_14390"
     CDS             4010..4759
                     /locus_tag="VFC01_14390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN80854.1"
                     /translation="MSDDARPVAVVTGGARGIGETIAEALIGAGYRTAVIDVNAKAIA
                     RAQESDAARAGLLVPLQLSVTDRPSVEHATQELAAEHGPIRALVNNAGMTLPATFLDQ
                     SDEDWDRIVDVNLKGTFVMAQVVGRQMVAERSGVIVNISSVSAHGIRTGPPAYAAAKA
                     GVEGLGRLMAVQLGPYGIRVNTMVVGTTATPWLLSRKSDDEIDEMRSSTLTGRIGEPD
                     DVARTVAFLCSPEAKHLTGQLISVSGGQWMP"
     gene            4836..5603
                     /locus_tag="VFC01_14395"
     CDS             4836..5603
                     /locus_tag="VFC01_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN80855.1"
                     /translation="MDLKRILFDVSDHVATITINRPERLNATDDLARTELGMAWGRVR
                     DDSDIRVAIITGAGDRAFCAGQDIRATAEGGIRNKVPGSRLHHNVWKPVICALNGMAV
                     GGALHQVADADLIIAAEHAELIDTHLQVGNVFALEPAVLLRRMPLSMVMQLALMTKEG
                     RITAQRGYEIGLINEVVPSERLQQRAREIAMAIAKLSPATVQASIKAMWSSLDVGLHA
                     AHDVAYRYVLQHQMTHPDYREGMVAFAEKREPRWVVE"
     gene            5615..7240
                     /locus_tag="VFC01_14400"
     CDS             5615..7240
                     /locus_tag="VFC01_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZN80856.1"
                     /translation="MTFHYDLLPYEAAMPAMLATVAAAHGSSDFVVSMHADGTVDRIT
                     YAEAETRSADMAARLLAAGVTKGVRVGILAPNGPDFAAAFLAITRIGAVAVPINTFFQ
                     PAELHWVLRDADVHTLLAVDMLLGKDVLERIEAAAGGWANADGPLRVERLPQLRNVLP
                     IGTGDRRWPSLWPDPVSTDFLRACESTVRPADDLLVIYTSGSTSNPKGIIHTHGTAIT
                     HSRFIATAHGWDGTDRIYVPMVFFWVAGLVFGFLGPMQVGATILTEHKFDAGDVLRLL
                     ESEKATYTTGFPHIGPALVNHPDFDATDLSSLREGYQQVLLPPERRTADPSLRVAQLG
                     MTETCSSHTWWPPHEQVPEAKRGSLGVAAPGFEHKVIDDVGNEVPSGVTGEICVRGTA
                     LLRGMIGKQWHEVVDRDGWLHTKDAGYRDDDGHLYFAGRTDEMIKTSGTNVAPMEVEA
                     ALGRIDAVRIAYVVGIPDPDKGAIVSAAVVLNEGRTATADDLVTACRSQIAAYKVPKR
                     WAILADAESLPYTTTNKIDKVRLTALMSAGELS"
     gene            7237..7896
                     /locus_tag="VFC01_14405"
     CDS             7237..7896
                     /locus_tag="VFC01_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN80857.1"
                     /translation="MTSLADLEARIERLEALDEIRQLAAKYAVALDMRDLDALVNLFV
                     EDVGVPGKRRGRAALRSWYDVEMRHDLLGSAHGVLGHVIDIHDADNATGLVYSRNDLE
                     TESVWVIEMLAYLDSYQRREGRWYFVKRIPLFWYESDITDPPVGGQKMRWPGTRRHDG
                     SLHDAFPSWQEFWNAEPGRLEAPVTAPAPVGAWLRTLRRDADDPRVDPTGRDVEDPRN
                     R"
     gene            7898..9022
                     /locus_tag="VFC01_14410"
     CDS             7898..9022
                     /locus_tag="VFC01_14410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80858.1"
                     /translation="MISSAEGFAATEEQQELRRSVRAFLELKSPESEVRRVMESPQGY
                     DPGVWRQMADQLGFQGLAIPEELGGTGFSFAELGVVIEETGRALLPGPFFGSAVLAGS
                     VLMCCDDDAKSRYLPGVASGETIATLALTEQSGRWDAEGIQTEARRKGSSWILNGTKT
                     FVLDGHIANLVLVAGRTAAGISLFAVDQTDSLTRRELSMLDPTRRQARIDFSDTPAEL
                     IGFEGLAWPALSHALDLAAAGLAVEQVGGAQRCLELAVEYAKTRRQFGKPIGSFQAIR
                     HKCADLMLDIECARGAAQYAVQAAASGAAELPAAASLAKAYCSEAYARAAAANIQIHG
                     GIGFTWEHPAHMYFKRAKSSGLLLGDAAYHRRLLADRVGI"
     gene            complement(9019..9828)
                     /locus_tag="VFC01_14415"
     CDS             complement(9019..9828)
                     /locus_tag="VFC01_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN80859.1"
                     /translation="MDLGVAGRNFVIVGGTTGMGFAAAEQLARDGANVALLARDGERA
                     AAKAKRLADAHDVSAVGISVDAAASGDGVERAVEMAADSLGPLRGLAVTAGPMKQQGP
                     FLEHGDDSWDWYYQMILMATVRSCRAIIPHLQRNGGGTVVTTAAYSVRAPKILIPPYN
                     ALKAAVLTLSKILAKTHGPEGIRVNTVCPGLFDTEVNDFIRARRAQEYNVPLQDAIYT
                     HLASNPAWNMRVALDRGGRPHEAGELIAFMLSDRAAYMTGAVINIDGGTDF"
     gene            10030..10641
                     /locus_tag="VFC01_14420"
     CDS             10030..10641
                     /locus_tag="VFC01_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80860.1"
                     /translation="MSTERVTAELDGQEESDRRDQILEAANLCFTQLGIQRTSVQDVA
                     RMANVSRGTVYRYFEDRTILIEAAIEFGAQKFYQEVAAAMAKKSTLAEKVGAMAETHA
                     KILIDHRTRNRLMADDAELMRHMISDGDSAVRRTTEFLVPYVREAQDRGEVGAGVDVT
                     AASEWLARIIYSFSTVNQAQTFDMSKPATVRRYVEKFAINGLR"
     gene            10724..11482
                     /locus_tag="VFC01_14425"
     CDS             10724..11482
                     /locus_tag="VFC01_14425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN80861.1"
                     /translation="MGERSLAGKRVVVVGASAGIGRAFAVRAGKEGAKLVLAARRADK
                     LDEVVAEAGVGTTVAVDVRNPADCSRLGEVAGETLGEVDILLISSGYAPLKMITDTDA
                     QDWCDVFETNVVGVHEIIRAHLHLLIPSAIVAALSSDSVRYPHTALGAYSSSKAAMER
                     CLVSWRMEKPGYRFSCVEISGTVPTDFTSAFDPDLLGTALTEWLARGLVQESRMTPEA
                     VADVLAGMFASAIDHPDVGLESIVIKSPTGPMRA"
     gene            11479..12759
                     /locus_tag="VFC01_14430"
     CDS             11479..12759
                     /locus_tag="VFC01_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HZN80862.1"
                     /translation="MTTQVESAWPSYPDYRIDVTRCPYVGQVWFGDLLLAESASCLVV
                     TETDHVDRLYFPEADVQWNLFTATEHTTVCPFKGEARYWSLSHGDRLIENVVWGYPNP
                     LPEVAGIAGHASFYDKDLRVVVVESWPDGSPVPAKFPLWGDATELVRLIDVEPVGAGR
                     FRGPAHGPTHRDVVEGGQLIAEAIVAASKTVPEQRVTSASMVFIKAASFTAPVDLNVE
                     LLRKGRTFSTAEVRATQDGTLRGAAILLCDSGAPDVIGHVVAMPDVASPSEAVPFEGF
                     AMPGREIRVVDAAYGQDPNRVGPPEINAWVRFRNAPDQPYLHAALLAQSTTHWTIAAG
                     MLPHAGFGEALAHRTLSTGIMKATVAFHEDVDVTDWLLYANRAFWAGRGLVQGDGHVF
                     TQDGRLAASHVIQAMVREFERDPASMGHDSRTAM"
     gene            complement(12987..13805)
                     /locus_tag="VFC01_14435"
     CDS             complement(12987..13805)
                     /locus_tag="VFC01_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN80863.1"
                     /translation="MVLQRDAVSLDGRIAVVTGGGAGIGAAIAAAFTEFGARVALWDK
                     DGAAATRTASKIGGLACATDVRDPDQVDAALANTIETLGPPSILVNNAGGVFFSRLLE
                     TSPNGWDALVRLNLTQVLLCTQRIARTMVDASLGGSIINVSTIESVRAAPGFAAYAAA
                     KAGVVNFTKTAALELAPHGIRVNGLAPDFTLTESLSRMAPAGEENAKLMVPLGRAGHV
                     DEMAGAAVFLASDLSSYVTGQTLHVDGGTAAAGGWYHHPNGGQYVLGHVAAVAQ"
     gene            complement(13831..14256)
                     /locus_tag="VFC01_14440"
     CDS             complement(13831..14256)
                     /locus_tag="VFC01_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN80864.1"
                     /translation="MTVPTRDDLVAYHELAEAKARYCRLLDTKDWASLADLLTADMEF
                     DLSDGNSDVAPIVGRENVLAAVQSSVAGAKTVHQIHVPEIDLNGDDAHVIWAVQERVV
                     WDNGTSLTAFGHYHDRWVRVDGKWKVAALRLTHLVMDFG"
     gene            complement(14253..15467)
                     /locus_tag="VFC01_14445"
     CDS             complement(14253..15467)
                     /locus_tag="VFC01_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN80865.1"
                     /translation="MNQDRASVEFFADATIQDPYPVYERMRAEGPVHRIGDSGFYAVC
                     GWDAIHDVINRPDAFSSNLTATMTYSPENGVAAFELDGLGGPTHVLATADDPLHAVHR
                     KLLVPQLAAKRIRAAEEFVIATADRLWNENVRDGRIEWMSAIANKLPMMVVARIIGVP
                     DDDVDMLIAAGYAMTQLLDGLIDGDGLAAAGAAAMEVSGYISEHFARAAENPGDDLLG
                     ALAAACAAGELDDTTARIMMLTLFSAGGESTASLLGSAVWILTRYPNVQRRLRSERNL
                     LGAFLEEALRYEPPFRGHYRHVVADTTLAGVALPAGSRLLLLWGAANRDPSQFASPND
                     FRLDRPHGKGHLTFGKGAHFCVGAALARLEGRIVLEMLLDRTQWIDAADTGRWLPSIL
                     VRRLERLQLAVR"
     gene            15591..16448
                     /locus_tag="VFC01_14450"
     CDS             15591..16448
                     /locus_tag="VFC01_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZN80866.1"
                     /translation="MNRLTGKVAVITGAARGQGRSHAVHLADEGADIIALDICADIES
                     NEYPLATPADLDETAKLIEKSGRRVVTGIVDVRDRGALKKVLDEAVAELGGLNVVVAN
                     AGICPQGNHIPYRGFIDAFDVDFVGVVNTVHLGLEHLTAGGSVIVTGSIAGLVEQKDL
                     ANGGGPSGPGGAGYGMAKKMVRDYTKALALTMAPHNIRINTVHPTSVNTHMLHNQPMY
                     RTFRPDLTDPTREDAELVFPILQAMPIPYVETEDVSHAVVYLASDESRYVTGQQLFID
                     GGAGLKMGV"
     gene            complement(16465..17079)
                     /locus_tag="VFC01_14455"
     CDS             complement(16465..17079)
                     /locus_tag="VFC01_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80867.1"
                     /translation="MRSRGWAGSTPASDEEAIARILDAVDEVVAEHGPVIRLADVARK
                     LGVTRQTVYHYFPNADALLIASAMRAVNGFIDQVADHVNGLNDPVTAVVESISFAVEN
                     LTGHPQLGRLLSQPEGDAAMSLTSDAAITFCLSIFHRLDVDWKLHGFDTNALAQLAEM
                     TLRTVQSLLIDPGQPPRDGLALRRFVAQWLGPAILHPRMASLSQ"
     gene            complement(17667..18470)
                     /locus_tag="VFC01_14460"
     CDS             complement(17667..18470)
                     /locus_tag="VFC01_14460"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HZN80868.1"
                     /translation="MCDQMRPKSYVDKPLVKPEPVTADDLRPAAEGRQDLSDAPSMAN
                     TSDSPLTPDVRPSTNSPRRFGQLPSLKVPDNFDDPLPDAETAAREVDSPSYAGDVANQ
                     DVGSASLRGLLVRDPYASRLLDGEKIWEIRGRSTHIRGPILIIKSGTGQVFGTADLVR
                     VLGPLELEDLVDASELPRQEREEFRRSGLPYPKTYAYVFSNPRRFNRPIPYRHPSGAV
                     TWVRLPDVDLGAVRYASSPHGASEGDLLHVLATEIWPLLERILGDPTAT"
     gene            complement(18421..19041)
                     /locus_tag="VFC01_14465"
     CDS             complement(18421..19041)
                     /locus_tag="VFC01_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80869.1"
                     /translation="MDELPSSLREYLDVWCADLLDHLTAARRDQVCGLVESSYAGGPW
                     PPRLMVQRMCELNTGCIDIDAFVGDVTTRWGCSIDDAVRMLSAEDCDDRANAIVLLGR
                     YPDTTELVEKVTAAHHAGTITDAEFNAVCSAALRRPLLPPPVPPPAHLPLLPADYPES
                     YAEFRRREPDFTPLPAEHTSTRDQGSCETAHVRSDATEELRRQAPS"
BASE COUNT         3557 a         6008 c         6078 g         3498 t
ORIGIN      
        1 atcgggtaag gcgtccgcgc gcatagtcga gcagctcgcc gggatcaacg tgcgccccgt
       61 cgaccagctc gaccgccccg accgggaccg aaccgagccg ttcgtcggga attcccgtga
      121 cacccgcgtc acgcaccgcg ggatggccac gaagcaccga cacgatgtcg gccggtgtca
      181 ccttgaaccc gccgcgaatg atcacatcgt cggtgcggcc gtcgacgaac aagaacccgt
      241 cggcatcgac tcggccgaga tcggtggtcc gtacccactc gccaccgcgc gtccgaacct
      301 cgatccgacc ctgagcaccc gtgggcacct cagcaccgtt ctcggcggag acgattcgta
      361 tctcacggcc gctctgcgga cgaccgacac tgccgcgctt ggcctttgcc cactcgcggt
      421 gaagcgcgag attccatccc gcgactccgc cggcgaactc ggtggcgccg tacgacggaa
      481 gcactgccac cccataccgt tcttcgaacg cctcctgtac cgaaaccgga aggggagccg
      541 taccagaggt cacgacctcc acgctggaga acacctccgt tggcacggtg gcgtccaaga
      601 ccatcgtcag cgccgatggg accagactca cggctctggg ccggtgacgg acaacgaggt
      661 cgacgaactc gggcacctga aaccgctcga ggagcgcgat cttgcggcct tcgcagatgt
      721 tcagcagcgt gcggaacaaa cccgacatgt gcaccaatgg cgaactgacg attgcgacac
      781 cggaacgcag gcgcagatcc gcaggtttgc tcgagccgta atgggcgccc gcggcggcga
      841 cagtatgctc aaatgcgtca tacgacagtg gaattcgttt cggcggcccg gtggtgccac
      901 tggtcagcat ctcgaccgcg acgccgtcgg gttcctgccg gaatggaccg ggacccactc
      961 gttccagacc gggaacggtc gcgacagcgg ccgggatcgc acctacttcg agacccagcg
     1021 aaccggttgg cgcggctgca atgccaggtc tacgccagtc ggcgtcaacg ccgatcagtg
     1081 cgggcagcat caactccgag atatctgcgg caagtcccga ttccccccgg gatgggttga
     1141 tggtgacgac gcagctgtcg gccaacagca cgccgagcat cgccgcgacc atcgctggat
     1201 ggtttcggag caccagcccg acaggcgatc ccgcacccaa acccgctgct atcaaatggc
     1261 tctcgacacc aaaggcgatg tcggagagcg cctgccaatc ggtccaggca ccctggtact
     1321 cgattgcggg agccatcgga tcgagcgcca ggatcgcacg gatgcggtcg gcaattggac
     1381 gactcaagcg ggggctgccg caagctcggt cgccgccggg atcacctctt gggcgaacag
     1441 tttcatcgac tcgtagacct tgccgcggtc catagcgcca ccacgagaca tccgcagcat
     1501 gatgttgcgc caccccatgt cgatgacggt acgcatcttc tcgatgatcc tgtccggatc
     1561 acccatcaac gtcagatgcg agttcagcac ctgctcgtag ctctgcttgt cgtgcttgtc
     1621 gaaccagtcg gagtacgcct tgtagtccgc cgggatgtcg ccaccacgtt cgaaggggtt
     1681 cgagtattgc cggtgcgtct gaatcgacag ctcgacgctg tcgcgcggat acgtccgggc
     1741 gaatccatcg tcggggtggc agaacgcatt cagcagggcc cagacgtttc cggtcgcggc
     1801 cgccagtccc tttttcgcct gggtctccag gtagatgtca agggctccct gtaggtcagg
     1861 gtcaacctgg tacgggttgc cgatgatggc accaaatccc ttgtcggcca gccactcgaa
     1921 gctcgacggc gtcttcatca ccgtgcccca caccggaatc ggatcctgca ccggccgcgg
     1981 gtggatggtc acgtcctcga gttgccagta cttgccctcg tacgagacgt tctcctgcga
     2041 caacagcaga ttgacgatct cgacgcactc ctggtagcga gcggtcgcct cgtccatcgg
     2101 gatgccgaag cccttgaact cgtgggcctg ataaccccgc ccgaagccgg cgtcgaagcg
     2161 cccaccgctc aggttgtcca ccatcgcaat gcgctccgcg aggtgaatcg ggtcatggaa
     2221 cggcgcgacc atgcatgcgg tgccgatccg gacacgctcg gtctgtgcgg caatcgcggc
     2281 cgcgaccaga ggcaggtccg cgagtgtgcc gtaaggcgag aagtggtgtt cggcgagcca
     2341 gatctcgtcg tatccgagtt cctcagccca ccgggcctcc tccagcacgt tggcgaagac
     2401 ctcttcgtgg ctgaggtgat tgggcttgtc gccgaggatg aatacgccga accgcatgcc
     2461 tgcgacctcc gtctcagaat atagttgcag ttactgccac tatattgcac gattgaacaa
     2521 atatcgtcaa tccgttccat cgtttggaga cctcgtgacc gcgcccgtga ccctctccga
     2581 cgccgtggtc ggcaccgctc tcgctccgac gacctaccgg tggacggaaa cggaagtcat
     2641 cctctacgcg ctgggggtcg gggcgcgtcc gccagcagaa ctgcggcttc tcgacgagca
     2701 ctgtgggcct gccgttcttc ccaccttcgc gctgatcgcc aactggtggg ccgtcaagga
     2761 tctgcgcgcg gcactgtccc tcggatcatt tccgatcgtg cattccgcgc agtcgctgcg
     2821 actcgaacgg ccggtgggcg ccagcggcga agtcagcgtc accgcagagg tgacggcggt
     2881 ctgggacaag ggcaagcacg cggccatcga agtgacatct gtcggcgtcg acgacgacgg
     2941 tcccctgttc agcgcctcct cgcagaccat ggtcctcggc gggggtggct tcggcggaga
     3001 ccggggccca tcggccgccg agcatccgca gggtgcgccc gacgcggtgt acgaggatgc
     3061 ggttcgcccg gagcaggcgg ccatctatcg gctcagcggt gaccgtaatc cgttgcacat
     3121 cgaccccgct gcggcacgca cgttcggctt cgatgatgtc tttctgcacg gcctgtgcac
     3181 acttggcttc gccgcccggg ccctgatcaa cgtcgtcggc gacggcgatc cgcagacgtt
     3241 gacgtcgatc agctgccggt tcgcgaaacc ggtcatgctc gattcggagc tgtgcacgga
     3301 gatatggcgc gatggcgacg aagtccgctt tcagaccctg cagggcgatg tcgtttcgct
     3361 cagttcgggc acggccacgt tggcaggatg ctgaggatgg accaacggcg cgcgcgaggc
     3421 cagcgcaacc gcgaggcact gatcgcggcc gcgatcgagc tgttcaccgc caacggatac
     3481 gaacagacga ccgtcgagca gatctcggag gctgccggcg tggcaccgcg aacgttcttc
     3541 caccacttcg ccgcgaagga cgacatcctt ttccacgggt acgccgaccg actcgaggag
     3601 gccacccgac gattccgcgc atcccgttcc ggctcgttgt gggatgcact ggccgaagca
     3661 tccgaagcgg tcgccacggc gatcagcgag acgccggaca tctttctggt ccgggccacg
     3721 atgtacggca gcctgcctgc gctgcgggcc acgatgctgc gcatcaacga cgactggatc
     3781 gatcagatga caaccgaagt ggcgcgttgg ttggacaccg acgcggaagt cgatgtgcgg
     3841 cctcgcctag ctgccacggt gatcaacggt gcaaaccgtg ccgcaatcga tctgtgggtc
     3901 gccggcggcg gcaaaggtga tctcattgcg atgatggcgg aagccgtgaa gttgatgcgt
     3961 ccgtcgatca actccatcga gcgcgaagcc aaacaggcgc ggggccgtcg tgtcggatga
     4021 cgcgcggccg gtcgcggtcg tcaccggcgg ggcacgcggc atcggtgaga ccatcgccga
     4081 ggcactgatc ggcgccggct atcgaaccgc ggttatcgac gtgaacgcga aggcgatcgc
     4141 gcgcgcccaa gagtccgatg cggcgcgcgc gggactgctt gtgccacttc agctgtcggt
     4201 aacggaccgc ccctccgtcg agcatgcgac gcaagagctg gccgcggagc acggcccgat
     4261 ccgcgcgctt gtcaacaacg cgggaatgac cttgcctgca accttcctcg accaaagcga
     4321 cgaggactgg gaccgcatcg tcgacgtcaa cctcaagggc accttcgtga tggcgcaggt
     4381 ggtgggtcgg cagatggtcg ccgaaagatc cggagtgatc gtgaacatca gctcggtgtc
     4441 ggcgcacggc attcgcacgg gcccgcccgc gtacgcggcg gcgaaggcgg gcgtcgaggg
     4501 cctcggccgt ctgatggccg tgcagttggg cccatacggc atcagggtca acaccatggt
     4561 ggtcggcacc actgccacgc catggctgct gtcacggaag tccgacgacg agatcgacga
     4621 aatgcgttca tcaacgctga cgggtcgaat cggtgagcct gacgacgtgg ctcgcacggt
     4681 cgcattcctc tgctcacccg aggcgaaaca tctcaccggt cagctcatct cggtctccgg
     4741 cgggcagtgg atgccgtgag gagacacaac ggaacgatga gacactttct gctactctgt
     4801 ttagggccag gcgcgcctcg aggccagggg attcggtgga tctgaagaga attctgttcg
     4861 acgtcagcga tcacgtcgcg acaatcacga tcaatcgccc cgagcgcctg aacgccaccg
     4921 acgaccttgc ccgcaccgag ctgggcatgg cctgggggcg agtgcgtgat gactccgaca
     4981 tccgggttgc gatcatcacg ggagccgggg atagagcgtt ctgcgccgga caggacatca
     5041 gagccacggc cgagggcggt atccgcaaca aggttcccgg atctcggctg caccacaacg
     5101 tgtggaaacc ggtcatatgc gcgctgaacg gcatggcagt cggcggcgcg ttgcaccagg
     5161 tcgccgatgc cgacctgatc atcgccgccg aacacgccga gctgatcgac acccatctgc
     5221 aggtcggcaa cgtgtttgcg ctcgagcccg cggttctgtt gcgtcgaatg ccattgtcga
     5281 tggtgatgca gctggcactg atgacgaagg aaggccgcat caccgcgcaa cgcggatatg
     5341 agatcggact catcaacgag gtggtgccct cggagaggct tcagcagcgt gcccgcgaga
     5401 tcgcgatggc gatcgcgaag ctgtcgccgg ccaccgtgca ggcgtccatc aaggcgatgt
     5461 ggtccagcct cgacgtcggg ctgcacgctg ctcacgatgt cgcttaccgc tacgtcctcc
     5521 aacaccagat gacgcatccc gactaccggg agggaatggt cgcgttcgcc gaaaagcgtg
     5581 agccccgctg ggtggtcgaa tgacattggc ggccatgaca ttccattatg atttgcttcc
     5641 atacgaagcg gcaatgcccg cgatgctcgc aaccgttgcc gcagcgcacg gctcaagcga
     5701 cttcgtcgtc tcgatgcacg ctgacggcac ggtggaccgg atcacctacg cggaggcgga
     5761 gacccgctcg gccgacatgg cggcccgcct cctcgccgcg ggtgtcacca agggcgtgcg
     5821 tgtcggaatc ctggcaccga acggccccga cttcgcagcc gcctttctgg cgatcacccg
     5881 catcggtgcg gtcgccgtgc cgatcaacac gttcttccaa cctgccgagt tgcactgggt
     5941 actccgcgat gccgatgtcc acacgctgct ggccgtcgac atgctgctcg gcaaggacgt
     6001 cctggagcgg atcgaagccg cggccggcgg ctgggccaat gccgacgggc ccctacgagt
     6061 cgagcggctc ccccagttac gtaacgtcct cccgataggc actggggatc gtcgctggcc
     6121 gagcctgtgg ccggacccgg tttcgacgga cttcctccgt gcgtgtgagt cgaccgtccg
     6181 accagccgac gatctgctcg ttatctacac ctctggaagc acgtcaaacc ccaagggcat
     6241 catccacact cacggcacag cgattaccca ctcccgcttc atcgcgaccg cgcacggttg
     6301 ggatggcacc gaccgcatct acgtaccaat ggtgttcttc tgggtcgcag gcctggtctt
     6361 cggcttcctg ggcccaatgc aggtcggggc gacgatcctg accgagcaca agttcgacgc
     6421 gggtgacgtt ctgcgtctac tcgagagcga aaaagcaacc tacacaacgg gttttccaca
     6481 catcggacct gctttggtga accacccgga tttcgacgcc accgatttgt ccagtctgcg
     6541 ggagggctat cagcaggtcc tgctaccccc tgagcggcgc acagccgatc cctcgttgcg
     6601 cgtagctcag ctgggaatga ccgagacctg tagttcacac acgtggtggc caccgcacga
     6661 gcaggtcccc gaggccaaac gtgggtcgct gggggtcgct gcgccgggct tcgagcacaa
     6721 ggtcattgac gatgttggca atgaggtccc cagcggggtc acaggcgaga tctgtgtgcg
     6781 cggaacggcc ttgctccggg gcatgattgg taaacagtgg cacgaggtgg tggaccgcga
     6841 cggctggctg cacacgaagg atgccggtta tcgcgacgac gatggccacc tgtacttcgc
     6901 gggacgcacc gacgagatga tcaagacatc aggtacgaac gtcgccccga tggaagtgga
     6961 ggcggcgttg gggcgcatcg acgctgtgcg catcgcctac gtggtcggaa tccctgaccc
     7021 ggacaagggc gcgatcgtca gcgccgccgt ggtgctcaat gagggccgga ctgcgacggc
     7081 ggacgatctt gtgaccgcat gccgtagcca aattgctgcg tacaaggtgc cgaaaaggtg
     7141 ggcgatactg gcagatgcgg aaagcttgcc gtacaccacc accaacaaga tcgataaggt
     7201 gcgactgact gcactgatgt ccgcgggaga actctcgtga catcgctcgc cgacctcgag
     7261 gcgcgcatcg aacgcctcga ggcactcgac gagatccgtc agcttgccgc gaaatacgcc
     7321 gtcgcgctgg acatgcgcga cctcgacgcg ctggtgaatc ttttcgtcga agacgtgggc
     7381 gtgccaggca agcgccgcgg ccgtgcggcc ttgcgttcct ggtacgacgt cgagatgcgc
     7441 cacgatctgt tgggcagcgc tcacggcgtg ctgggccatg tcatcgatat ccacgacgcc
     7501 gacaacgcca ccggcctcgt ctattcacgc aatgatcttg agacggagtc ggtctgggtg
     7561 atcgagatgc ttgcttatct cgacagttac caacgccggg aaggtcgctg gtacttcgtc
     7621 aagcggatcc ccttgttctg gtacgaaagc gatatcaccg acccacctgt gggcgggcag
     7681 aagatgcgat ggcctggtac ccgtcgacat gacgggagtt tgcacgatgc gtttccgtca
     7741 tggcaggagt tttggaacgc cgaacctggc cgcctcgagg ccccagtgac cgcgcccgcc
     7801 ccggttggcg cgtggctacg caccttgcgc cgcgacgccg acgatccccg ggtagatccc
     7861 accggacgcg acgtcgagga cccgaggaac cgttgagatg atcagttccg ctgagggttt
     7921 cgcggcgaca gaggagcaac aggagttgcg ccggtcggtc cgggcgttcc ttgaactcaa
     7981 gtcgcctgag agcgaagtac gccgcgtgat ggagtccccg caaggttatg accccggcgt
     8041 gtggcggcag atggctgatc aacttgggtt tcagggtttg gccattccgg aggaacttgg
     8101 cggaactggc tttagcttcg cggaactggg cgtggtcatc gaggaaaccg ggcgagcgct
     8161 cctgccgggg ccattcttcg gctccgccgt gttggcgggc agcgtcctga tgtgctgcga
     8221 cgacgacgca aagtcgaggt atctcccggg cgttgcgtca ggtgaaacca tcgcaacgct
     8281 ggcgttgacc gagcaatccg gccgatggga tgccgaaggc attcagaccg aagcgcgccg
     8341 aaagggatca tcgtggatcc tcaacggcac caagactttc gtgctcgacg ggcacatcgc
     8401 gaacctggtg ttggtcgcgg ggcggaccgc cgcaggcatc tcgctgttcg ccgtcgacca
     8461 gaccgacagc ttgacccgcc gtgagttgtc gatgctcgat ccgacgcggc ggcaggcacg
     8521 tatcgacttc tccgataccc ctgccgaact aatcggcttc gagggactgg catggcccgc
     8581 gctatcgcac gcactggatc tcgcggcggc cggtctcgcc gtcgagcagg tggggggcgc
     8641 gcagcgatgt ctagaacttg ccgtcgagta cgcaaagacc cgccggcagt tcggtaagcc
     8701 catcggatcg ttccaagcga ttcggcacaa gtgtgccgac ttgatgttgg acatcgaatg
     8761 cgcgcgcggg gcagcccaat acgcggtaca ggccgcggct agcggggcgg ccgaactgcc
     8821 ggccgctgcg tctctggcga aggcttactg ctcagaggct tacgcccgag ccgcggcggc
     8881 gaacatccag attcacggtg gaatcggatt cacctgggag catcccgcac atatgtattt
     8941 caagcgcgcc aaatcgtccg ggcttctact tggcgacgcc gcataccatc gccgactgct
     9001 cgctgatcgc gtcggcatct agaagtctgt accaccgtcg atgttgatca cagcgccggt
     9061 catataggcg gcccggtcac tgagcatgaa tgcgatcagt tcgccggcct cgtgcggtcg
     9121 cccgccgcgg tcgagagcca cacgcatgtt ccacgccggg ttgcttgcga ggtgcgtgta
     9181 gatcgcgtct tgcagcggca cgttgtattc ctgtgcgcgc cgagcacgga tgaagtcatt
     9241 cacctcggtg tcaaacaagc caggacacac cgtgttcacc cgtatcccct ccggcccatg
     9301 ggttttggcc agaatcttgg acagcgtcaa cacagccgcc ttgagcgcgt tgtacggagg
     9361 gatgaggatc ttcggcgcgc ggaccgaata cgcggccgtg gtcaccaccg ttcctccgcc
     9421 attgcgctga agatgcggaa tgatcgcgcg gcaagaccgc accgtcgcca tgagaatcat
     9481 ttggtagtac cagtcccacg agtcgtcacc gtgttccagg aacgggccct gctgtttcat
     9541 cggccctgcg gtgaccgcca agccgcgcag aggccccagg ctgtcggcgg ccatctcaac
     9601 ggcgcgctcc acgccgtcac cgcttgctgc cgcgtcaaca ctgatgccga cggcactcac
     9661 gtcgtgcgcg tcagccaatc gcttggcctt cgcggcggca cgctcaccgt cacgcgccag
     9721 cagcgcgaca ttcgcgccat cgcgcgcgag ctgctcggcc gcggcgaacc ccatgccggt
     9781 ggttccaccg acgatgacga agttacgccc ggcgaccccg aggtccatgc cgagtccctc
     9841 cttgccagcg catcctgcgg aacgccacta aggtggcagg gtaaacagtt ggacgctgca
     9901 tgtcactatg accacccgat cccgctggct gtcgcggcta ccaggtgcgg tgcgccggaa
     9961 caccggaaca gtaggtgtcg cgtagcatag aaggcttgga gcaggcagat ttccgggacg
    10021 aggagcgaca tgtcgactga gcgggtcacg gccgagctcg atgggcagga agagtccgac
    10081 cgccgagacc aaatcctcga ggcggcgaac ttgtgcttca ctcaactcgg aatccagcgc
    10141 actagcgtgc aggatgtcgc ccgcatggcc aatgtgtcgc gcggcacggt gtatcggtac
    10201 ttcgaagacc ggacaatcct catcgaagct gctatcgagt tcggcgccca gaagttctat
    10261 caggaagtcg ccgcggcaat ggcgaagaag tcgacactgg cggagaaggt cggcgcaatg
    10321 gccgagaccc atgccaagat cctgatcgat catcgaaccc gtaaccggtt gatggccgac
    10381 gacgccgagt tgatgcggca catgatttcg gacggtgact cggccgtccg acgcaccacc
    10441 gagtttctag tgccatacgt gcgggaggcc caggaccgtg gtgaagtagg ggccggtgtc
    10501 gacgtcaccg ccgccagcga atggctcgcg cggatcatct actcgttctc gacggtgaat
    10561 caggcccaaa ccttcgacat gtccaagccc gccacggtgc gccgctacgt cgagaagttc
    10621 gcgatcaacg gcttgcgctg aggtgcggcc agctatggtc agttagacac attctgtaat
    10681 actgttctct acgacaacgt cggagaacga ggcagagggt cccatgggtg agcgatcgct
    10741 ggccggcaaa agggtggtcg tggtgggcgc ttcggccggt atcggacgcg cattcgccgt
    10801 cagagcaggc aaggagggcg ccaagctggt cctggctgcg cgacgagccg acaagctgga
    10861 cgaggtggtc gccgaggccg gtgtcggcac gaccgtggcc gtggacgtcc gcaatccggc
    10921 cgattgcagc cgtctcgggg aggttgccgg ggagacactc ggagaggtcg acattctctt
    10981 gatctcgtcg ggatacgcgc cgctgaagat gatcaccgac accgacgcgc aggactggtg
    11041 cgatgtgttc gagacgaacg tggtcggcgt tcacgagatc atccgcgccc atctgcattt
    11101 gctcattccg tcggcgatcg tcgcggcgct gtcgtcggat tcggtccgct atccacacac
    11161 cgcgctcggt gcctactcgt cgagcaaggc tgcgatggag cgatgcctcg tgtcgtggcg
    11221 catggaaaaa cccgggtatc gcttcagctg cgtggaaatc agtggcacgg tgccgacaga
    11281 cttcacctcg gcattcgatc ccgatctgct cggcaccgcg ttgaccgaat ggcttgcacg
    11341 cggtctcgtc caggagagcc gtatgacccc ggaagccgta gccgatgttc tggccgggat
    11401 gttcgcgagc gccatcgacc accccgacgt gggtctggaa tccattgtca tcaaatcgcc
    11461 gacaggaccg atgcgggcat gacgacccag gtcgagtcgg cgtggccgtc ataccccgac
    11521 taccggatcg acgtcacgcg gtgcccctat gtcggccagg tctggttcgg cgacctcctc
    11581 ctcgccgaga gtgcgagttg tctcgtggtc accgaaaccg atcacgttga ccggctgtac
    11641 ttcccggagg cggacgtgca atggaatctg ttcactgcga ccgaacacac cacggtgtgc
    11701 cccttcaaag gcgaagcgcg ctattggagt ctctcccatg gcgaccgatt gatcgagaat
    11761 gtggtctggg gctatccgaa tccgctgccg gaggtggcgg gcatcgccgg ccacgcttcg
    11821 ttctacgaca aggacctgcg cgtcgttgtc gtcgaaagtt ggccggacgg atccccggtg
    11881 ccggcgaagt ttccactttg gggtgacgcc accgagctgg tacggctcat cgacgtcgaa
    11941 ccggtcggcg cgggtcgatt tcgtgggcct gcacacgggc cgacgcatcg cgatgtggtc
    12001 gaaggcggcc agctgatcgc cgaagccatc gtggccgcat ccaaaactgt tccagagcaa
    12061 cgggtcacgt cggcatcgat ggtgttcatc aaggcggctt ctttcacggc accggtcgat
    12121 ctgaacgtcg agttgttgcg aaaggggcgg accttctcca ccgctgaagt ccgcgcaacc
    12181 caggacggca cacttcgcgg ggcagcgatc ctgctgtgcg attccggcgc tccagacgtg
    12241 atcggtcacg ttgtcgcgat gcccgacgtc gcgagcccgt cggaagcagt gccgttcgag
    12301 ggattcgcca tgcctggacg cgaaatccgt gttgtcgatg cggcctacgg tcaagatccc
    12361 aaccgtgtcg ggccgccgga gatcaacgct tgggtgcgct ttcgcaacgc gccggaccag
    12421 ccgtacctcc atgccgcgct gcttgctcaa tcgaccaccc attggacgat cgccgcagga
    12481 atgctgccac acgccggatt cggggaggcc ctcgcgcacc gcacgctgtc gaccggaatc
    12541 atgaaggcca cggtcgcctt tcatgaggat gtcgatgtca cagactggct gttgtatgcg
    12601 aatcgtgcgt tctgggccgg gcgcggccta gtccagggtg acggtcatgt gttcacccaa
    12661 gacgggcgac ttgcggcttc gcatgtcatc caggcgatgg tgcgagagtt tgagcgcgat
    12721 ccggcgtcta tgggccacga cagccgcacg gcgatgtgac atcgcaatcg acattcgtac
    12781 cgcgacgtgt cacgcggacc tcgaaacgct ctcgcgccgc tatgccgccg cgcgggatcg
    12841 tcgtgatcgt gatgccctgt tgagcgtctt cgacaccgac gcatcgatgc gggtagaaca
    12901 actcgggccg gagaacaagc agaggacgaa ttggccatgc tggtgtcggc cgtcggtgcc
    12961 gcgacagggc gttgaccgat ctactctcac tgagcaactg ccgccacatg cccaagcacg
    13021 tattggccac cattcgggtg gtgataccag ccaccggcag cagcggttcc tccgtccacg
    13081 tgcagcgtct gcccggtgac gtaagagctc aggtcgctcg cgagaaacac cgcggcaccg
    13141 gccatctcgt caacatgtcc agcacgaccg aggggcacca tcagcttcgc attctcctct
    13201 cctgcgggcg ccatcctcga caggctttcg gtcagtgtga agtcgggagc cagcccattg
    13261 accctaatac cgtggggggc gagctccagc gcagccgtct tggtgaagtt gacgacccct
    13321 gccttggcgg ccgcgtacgc ggcgaaacca ggcgctgctc ggacgctttc gatggtgctg
    13381 acgttgatga tgctgccgcc caacgaggcg tccaccattg tccgcgctat tcgctgggtg
    13441 cacagcaata cttgcgtgag gttgagccgg accagcgcgt cccagccgtt cggagaagtc
    13501 tcgagaaggc gagaaaagaa gaccccgccg gcgttgttca ccaatatgct cggtgggccg
    13561 agggtttcga tggtgttggc gagggcggcg tccacttggt cggggtcacg tacgtcggtc
    13621 gcacaggcga gtccgccgat tttggacgca gtgcgcgtgg ctgccgcgcc gtccttgtcc
    13681 cacagcgcga cgcgggcacc gaactcggta aaggctgccg cgatggctgc accgattccg
    13741 gcaccgccac ccgttaccac ggcgatccgg ccgtcgagtg acaccgcatc acgctgcaat
    13801 accattcgac ctccgtgcgc tttgcggtcg ctatccgaag tccatcacca gatgagtgag
    13861 ccgcagggcc gcgaccttcc acttgccgtc gacacgcacc caacgatcgt ggtagtggcc
    13921 gaacgctgtg agcgacgtac cgttgtccca tacgacccgt tcttgcacgg cccagatgac
    13981 atgcgcgtca tcaccattca gatcgatctc tggtacgtgg atctggtgga cagtcttggc
    14041 tcccgccacc gacgactgga cagccgccag cacattctcg cggccgacaa tgggtgccac
    14101 gtcggaattg ccgtcgctca agtcgaattc catgtcagct gtgagcaagt cagccagcga
    14161 cgcccagtct ttggtgtcca gaaggcggca gtagcgcgcc tttgcctcag ccagctcgtg
    14221 gtaggcaacg agatcgtcgc gcgtcggaac agtcatcgga cagccaactg cagtcgttcg
    14281 agacgccgca cgaggatgct gggcagccat cgtccggtgt cagccgcatc aatccattgg
    14341 gtacggtcga gcagcatttc cagcacgatc cggccctcca gtcgggccag tgctgcaccg
    14401 acgcagaagt gcgcaccctt cccgaacgtg agatgacctt ttccgtgcgg tcggtctagt
    14461 cgaaagtcgt tgggagatgc gaactgagaa ggatctcgat tggcggcacc ccatagcagt
    14521 agcaggcgtg accccgcggg caacgcgacg cccgcgaggg tcgtgtcagc cacgacgtgc
    14581 ctgtaatgac cccgaaaggg cggctcgtag cgcaaggcct cttcgaggaa cgcgcccaac
    14641 agattccgct cgctacgcag tcggcgctgg acattgggat agcgagtgag gatccagacg
    14701 gcgctgccaa gaagcgatgc cgtggactct ccgccggcac tgaacaacgt aagcatcatg
    14761 atccgcgccg tggtgtcgtc cagttcgcct gcagcacacg ctgctgctaa ggcgccgagg
    14821 aggtcgtcgc ctggattctc ggccgcgcgc gcgaagtgct cactgatgta accgctgacc
    14881 tccatcgcgg ccgcgcccgc cgccgctaac ccgtcgccat caatcaagcc gtcgagaagt
    14941 tgcgtcatcg cgtagcccgc tgcgatgagc atgtcgacgt catcgtctgg tacgccaatg
    15001 atcctggcca ccaccatcat cgggagcttg ttcgcgatcg cactcatcca ttcgattcgg
    15061 ccatcgcgca cattttcgtt ccacaggcgg tcggctgtcg caattacgaa ctcctcagca
    15121 gcacggattc gtttagccgc cagttgaggt accaacagct tccggtgtac cgcgtgaagc
    15181 gggtcgtcgg ctgtcgccag gacgtgggtc ggaccaccaa gtccatctag ttcaaacgcc
    15241 gcgacaccgt tctcggggct ataggtcatt gtggcagtca ggttcgacga gaacgcgtcg
    15301 ggccggttga tcacatcatg gatcgcgtcc cacccacata ccgcatagaa ccccgagtcc
    15361 ccaatgcggt gcacgggtcc ttctgctcgc attctttcgt agaccgggta gggatcctga
    15421 atcgtggcgt cggcgaagaa ctccacgctg gcgcgatctt gattcacagt cacggaatgc
    15481 agactctcca agcgtcacgg cggacacacc agggcatgtg acagattaac actcagtgtt
    15541 ctattatcca accacggtga gtgcttacat catcgcgtac ggagggttgg atgaaccgtc
    15601 tcacgggcaa ggttgccgtc atcaccggtg cagcgcgcgg gcaggggcgc agccatgctg
    15661 tgcacttggc agacgagggg gccgacatca tcgcgcttga catctgtgcc gacattgagt
    15721 ccaacgagta cccgttggct acaccggccg acctggatga aacagcgaaa ctgatcgaaa
    15781 agtcgggacg gcgggtcgtc accggcatag tcgatgtccg tgatcgcgga gcgctgaaga
    15841 aggtgctcga cgaggcggtc gcggagctcg gcggcctcaa cgtggttgtc gcgaacgcgg
    15901 gaatctgccc acaaggcaac cacattccct atcgcggatt catcgatgct ttcgacgtcg
    15961 acttcgtcgg agtggtcaac accgtccacc tcggactcga gcatctgacc gcagggggat
    16021 cggtgatcgt gaccggatcc atcgctggcc tggtcgagca gaaggatctg gcaaacggcg
    16081 gcggcccttc tgggccgggt ggcgccggct atgggatggc gaagaagatg gttcgcgact
    16141 acaccaaagc gctggcgctg acgatggcgc cgcacaatat tcggatcaac actgtgcacc
    16201 ccacgagcgt caacactcac atgttgcaca accaaccgat gtaccgcacc ttccgcccgg
    16261 atctcaccga tccaacccgt gaggacgcgg agctcgtctt ccccatactg caagcgatgc
    16321 caattccgta cgtcgaaacc gaggacgtct cgcacgccgt cgtttacctc gcttccgacg
    16381 aatcccgcta tgtcacagga caacaactgt tcatcgacgg cggggccggt ctcaaaatgg
    16441 gtgtatagcg gttagaccgg ctttctattg cgacagcgac gccattcgcg ggtggaggat
    16501 cgcggggcct agccattgag caacgaagcg gcgtagtgcg agaccgtcgc gcggtggctg
    16561 cccaggatcg atgagaaggg actgaactgt acgcagggtc atttcggcga gctgtgcgag
    16621 cgcgttggtg tcgaagccat ggagcttcca gtcgacatcg aggcggtgga aaatcgacaa
    16681 gcagaaagtg atcgctgcgt cggaggtgag ggacatggca gcgtcgccct ccggctggct
    16741 aagcaggcgc cccaactgtg gatgaccggt gaggttttcg actgcgaacg agatgctttc
    16801 gactactgca gtgaccgggt cgttaaggcc gttgacgtgg tctgcaactt ggtcgatgaa
    16861 cccgttgact gcgcgcattg cgctagcgat gagcagcgcg tcggcgttgg ggaagtagtg
    16921 gtaaaccgtc tggcgagtga ccccgagttt ccgggcgacg tcggcaaggc gtatcactgg
    16981 tccgtgttcg gcgaccactt cgtctaccgc atcgagaatt cgggcgattg cctcctcgtc
    17041 cgaggcgggc gtcgatcctg cccagccgcg actacgcatc cgctttcaca aacatttcgc
    17101 tgtcgaactc gatctcaagt gttgtggggc cggtcatttt tccagcagat ttgtccccta
    17161 ggcccaagca gttcgaacaa tcttcgagga tgctatctgg gtgcgtcgcg tgagccgccg
    17221 cgttctgccg gtatgcactc cgcgtgttac gcgccaaaca ccaccggacg tctttaaaaa
    17281 gcgcctctga actgcgccta tacggtggag ctaaggcgac tcgaacccct acggattgct
    17341 cgtgaaatat cgtctgagct gcggcgatgg ttcttccggt gagttacgcg acgccgccgt
    17401 gtactggcga tatgcctcgg tgtattacgc gacgtgacag tgctggcggc cgctttgcgg
    17461 gatacacctc acaggttcca cacccgcggt cggtggccaa cctcgaagat ggccgcttgt
    17521 agagccgttg tgtcagcggt cgcatcaact tctcgggtgc cgtcgaagtg ctttcccttg
    17581 agtgatgatc gccccgtccc gccaccaccg atgacggcca aatatgttgg gctgaaacat
    17641 agccgctgat ggagcccatc gcggactcac gtcgccgtcg gatcgccgag tatccgctcg
    17701 agtagtggcc agatctcggt tgccagcacg tggagcaggt cgccttcaga tgccccgtgg
    17761 ggggacgaag cgtagcgaac ggctcctaga tcgacgtcgg gcagccgtac ccacgtaacg
    17821 gcaccgctgg gatggcggta cggtatcggg cggttgaacc gtctcggatt gctaaagacg
    17881 taggcgtacg tctttggata gggaagtccg ctgcggcgaa actcctcacg ctcctgacga
    17941 ggcagctcgg atgcgtcgac aaggtcctcg agttcaagcg gacctaacac acggacgaga
    18001 tccgctgtgc caaacacctg tcctgtgccc gacttaataa tcaggattgg cccgcggata
    18061 tgggttgatc ggccgcgaat ctcccagatt ttttctccgt ccagaagccg ggaagcgtac
    18121 gggtcacgca ccagaagacc tcggagactt gcactcccca catcctgatt ggcgacatca
    18181 ccggcatacg acggtgaatc aacttcgcgc gcagcggttt ccgcgtcggg tagcggatca
    18241 tcaaagttgt cagggacttt gagactcggg agttggccga atcgccgcgg ggagttcgtg
    18301 gacgggcgca cgtcaggtgt cagaggactg tccgacgtgt tcgccatcga cggtgcgtcg
    18361 ctcaggtcct ggcgaccttc agcggcgggt cgcaagtcat ccgctgtcac cggttctggc
    18421 ttaactaggg gcttgtcgac gtagctcttc ggtcgcatct gatcgcacat gggcggtctc
    18481 gcaacttccc tgatcgcggg tggaggtatg ttcggcgggc aaaggtgtga agtcgggttc
    18541 gcggcgacga aattcggcgt acgactctgg gtagtcggct ggcagtagtg gcagatgcgc
    18601 tggaggcggt acaggcggcg gaagtagcgg ccggcgaagc gctgcgctac agacggcgtt
    18661 gaattcggcg tctgtaatgg tgccggcgtg gtgcgccgca gtcactttct cgacgagctc
    18721 agtggtgtcg ggatagcgtc ccaacaggac tatcgcattg gcgcgatcat cacagtcctc
    18781 agcggacagc atcctcacgg cgtcatcgat gctgcatccc catcgtgtgg ttacgtcgcc
    18841 gacgaacgca tctatgtcga tgcagccagt gttgagctca cacattcgct ggaccattag
    18901 tcgtggtggc cacggcccgc cggcatagct ggactcaacg aggccgcaca cttggtcgcg
    18961 ccgcgccgca gttaggtgat caagcagatc agcgcaccag acgtcgaggt actcccgcag
    19021 tgatgagggc agctcatcca tttgtgctag acgcgtcctt tccgtccccc gcagttccga
    19081 tcgtccgaag cggacggttc gccaggcgag gcgggtgggc tcggggcagg ccgaggtgag
    19141 t
//
LOCUS       DASRJA010000329         8391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_183718,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000329 DASRJA010000000
VERSION     DASRJA010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183718"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFC01_14470"
     CDS             <1..626
                     /locus_tag="VFC01_14470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN80870.1"
                     /translation="TRIQQSTVREGKAEGVWGLLRRRGMPAALMTSFSAKSGIDLLLV
                     YVPLLGVAVGLTSSQVGILLGISSSGALLARAATPFFVRRIPNVRLTVVATVVAASCL
                     LVIAVSGNLAPMMVAMSVLGFALGLSQTTTMDWVVNLVGDTSRGSALGLRVATNRVGQ
                     AFILAAAGAISEVWGVEAAFALLAAVMFVTAASGLASARRGPQSEGA"
     gene            complement(608..1414)
                     /locus_tag="VFC01_14475"
     CDS             complement(608..1414)
                     /locus_tag="VFC01_14475"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN80871.1"
                     /translation="MQKTRPESETGPAYPIASVNNALLLLLLFREQPRVRLTDACKYL
                     GVAHSTAHRLLAMLAHHGFVQQEPVTRAYIAGPALVEVGLAVVGSLNVREQARPAMEE
                     LAAETGETVHLGALEGNKVRYVDGLESERALRVVARTGTLAPAHCTSLGKALLAQMTD
                     EQVAKLYPTSAEPFESSTAQSITTQAKLLKELGKARQRGYAVNAGETEEDVGSVAVAF
                     RDFAGRPAAIAVAAPTSRLSAQRISRIGELMIETISRTPMAPGAAQAPSD"
     gene            1568..2155
                     /locus_tag="VFC01_14480"
     CDS             1568..2155
                     /locus_tag="VFC01_14480"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HZN80872.1"
                     /translation="MEQEIVRRPDEDTPDEDAAEELAAEPSPADGDQNRDARLTRRVH
                     VVLGLVIAALGAWLLYKSRTELEFRGPNNEPGPGYLPVLLTICLIALGLALSAVWLWG
                     PKARRGEAPTLSLSPQGIGRALLVWLALLVCAFLIEPLGFLAAGEVLVVLVIVVIERI
                     RSIPLIIALLLLPPAMYLLFDTLLEVQLPGGTLWY"
     gene            2175..3686
                     /locus_tag="VFC01_14485"
     CDS             2175..3686
                     /locus_tag="VFC01_14485"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZN80873.1"
                     /translation="MNAVEGLLHGLQSAITPTNLLWLLVGCLLGTLVGILPGLGPPAT
                     IAILLPLATGFAPATGLIMMAGIYYGAKYGGSTTSILLNIPGESSSVVTCIDGYEMAK
                     QGRAGKALGIAAIGSFVAGTLGVIGLTFLAPAAADVAVNFGPPEYSALMVFALLLVIM
                     LAGDSMLKGFIAMFIGLFLSTIGTDLFSGAQRFTGDQIELAGGIEFIALSVGIFAVGE
                     VLINIEDKTEKQLFQVPNKIRDLLPSRQDIKKSTPAMLQGGIVGFLIGILPGAGSTVA
                     SFVSYIIAKKTSKTPEEFGKGSIEGVAAPEAANNSETGGAMVPLLTMGIPGSGTGAVL
                     LGALVLYGLNPGPLLFHEHADVVWPIIASMYLGNIALLVMNLPMVPLFAKLLNTPYKV
                     LYPAILLISVVGVYSVNFSIFDVGMLVVFGLLGYVMRKLDIPPAPLVLAFVLGPIAEN
                     AIRQSLLLSNNSPIIYLERPISATLIGLAVALLLVLAFGRGRKVRDQVVEIDA"
     gene            3716..4705
                     /locus_tag="VFC01_14490"
     CDS             3716..4705
                     /locus_tag="VFC01_14490"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN80874.1"
                     /translation="MFRIRQLLTVAIAVLGLIALAGCGSSGGGGKSGPVEVVTHNAVG
                     GGSDVFTRQMIKVMYENKIIDTNWPVRNVPAGDSIGAMSYMFDHKGNAGLIAQVTPTW
                     LATPMTVENAKVSLAGLTPITLVATEPQVVATNAKSQFNTFADFVAAAKKAPDTLVQA
                     GGSSTANDALTRLVLQDSLGAKWKFLSFEDTGSRITALLRDDANIMLGSASDVAEQVK
                     AGQLKVIAVIGNQRLDAFPDVSTTEEQGIDSSKVPVQFRAVMGAPDMPADAVQKYQDD
                     LSKMVETDDWKNLIKDDGLVTQNLQDDKLETYLKEQTDIVGGLLQGLGLRKDQ"
     gene            4702..6063
                     /locus_tag="VFC01_14495"
     CDS             4702..6063
                     /locus_tag="VFC01_14495"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZN80875.1"
                     /translation="MTSAGPTSTLATFVAEQQSEQVPEPVMHAARRALVDHIGVTVAG
                     STDDATRRARDAIAASESGGGATVVGARMRVSPPFAAFLNAFASHVLDLDDVYNPPGT
                     TVHGSCSVWPAILAIADNRPVSGRDALASFALGFEVETRVAHAAGQTHYDVGWHVTGT
                     SGHVGAAAAAARTMGLDRNQIEHAMAAGATQAAGLRIMAGSDLKSMHPGKAAMDGVLA
                     ATLVEHQLTASAKAIEGDFGYLAVMSAAPAPEKITAGLGESWNLTSNGHKLYPSGSLT
                     HPMVDGVIALVTENGIAPDDVTVIDVRVSPPAARFTDRPRPATPMEAKFSLRHCAAAA
                     VVFRRLGTDELSAEVLARPDVIDLRDRVKVVADPAIGKQDADVEITLASGRTLSTEVR
                     GNRGTPSAPLADDELNVKFLELVAPVLGAERAERLLATCWQLDELPDVSVLLQQTVPA
                     ADG"
     gene            6056..6748
                     /locus_tag="VFC01_14500"
     CDS             6056..6748
                     /locus_tag="VFC01_14500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN80876.1"
                     /translation="MADIALVTGAGSGLGAVIAERLAREGLTVAVNTRAHTEEADAVA
                     AAIRAGGGSAFAVTANVTDPSAVRRMFETVAAHGTLRVLVNNAAYRPRQRVQAITEDD
                     WHQVRSVTLDGAFRCIRCALPALIPGGRIINILGRNALAGDPERVHLSAAKHGLLGLT
                     LALAKALRDDGVAVNAVSPGVDTDGPELDRCRAEIASQVARLAFVDAKELTGTVVRVD
                     CDGSVVSVPTAW"
     gene            complement(6726..8105)
                     /locus_tag="VFC01_14505"
     CDS             complement(6726..8105)
                     /locus_tag="VFC01_14505"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZN80877.1"
                     /translation="MGSRSQSYDDRTSAETRTLLDFVCHTAASDIPAEVLHESTRCLL
                     DHVGLAVAGAEEPAARIAREQCQALGGDPQATVLGTPHRLRVTDAALVNGISCHALDF
                     DDTHVPTILHPTTPLYASGTPLAQWRGAKGADLLAAHALGYELAARASNALYPEHYDA
                     GWHMTGTTGALASATVAIRLLGLTGTTATHCLSIAATQAAGHREQFGTMTKPFHAGHA
                     AAAGVWAGLLAAGGFTGAPDPLQGRRGMFAVMSSASTPADLVDGLGQRWQIFDNGVKP
                     YACGVVIHPAIDAVRDLAVRRGLTVGRIEKIRLRVHPLVLELTGKTDPRTGLEGKFSV
                     TFACSIALLDGRAGEAEFSDAAVVRADVRDLMSRIEVVPDADVPHTQAGATALTDDGE
                     TVETWVDHARGTPGNRLTDDGLREKFHGLTDGVLGRDRAERLADSVFALRADGDVEAM
                     LALTTPSGR"
     gene            8155..>8391
                     /locus_tag="VFC01_14510"
     CDS             8155..>8391
                     /locus_tag="VFC01_14510"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZN80878.1"
                     /translation="MGDRRSVSGALAAFAAGIRVENLPDDVVHQAVRCLVDWLGCTIA
                     GAATPESARVRAAIRAMEPDGSRAAGIVGTLDRGS"
BASE COUNT         1349 a         2816 c         2790 g         1436 t
ORIGIN      
        1 gcacccggat ccagcagtcg acggtgcgcg agggcaaggc cgaaggagtg tggggtctgc
       61 tgcgcagacg gggcatgcct gcggcgctga tgaccagctt ctccgcgaag agcggcatcg
      121 acttgctctt ggtctatgtt ccgttgctcg gtgtcgccgt cggtctgacg tcgtcacaag
      181 tcggcatcct gctgggcatc agctccagcg gtgcgctcct ggcgcgggcc gcgaccccgt
      241 tcttcgtccg ccggatcccg aacgtgcgac tcaccgtggt cgcgacggtc gtcgcggcgt
      301 cgtgtctgct cgtaatcgcg gtcagcggca acctggcgcc gatgatggtc gcgatgtcgg
      361 tgctcggctt cgcgctcggc ctgtcgcaga ccaccaccat ggactgggtc gtcaaccttg
      421 tcggcgacac cagccggggc tcagcgctcg ggctgcgagt cgcgaccaac cgcgtcgggc
      481 aggcgttcat cctcgccgca gcgggggcca tatcagaggt gtggggagtc gaggccgcct
      541 tcgcgctgct ggctgccgtc atgttcgtga ccgccgcctc gggtctcgcc agcgcgcgcc
      601 ggggccctca atccgagggt gcttgagcgg cccccggcgc catcggcgtt cgggagatcg
      661 tctcgatcat cagctcgccg atgcgcgaaa tccgttgcgc gctaagccga ctcgtcggcg
      721 cggccaccgc gatcgctgcg ggccgccccg cgaagtcgcg gaatgcgacg gccaccgagc
      781 ccacgtcctc ctcagtctcg cccgcgttga cggcatagcc ccgctgtctg gccttgccca
      841 attccttcag caacttcgct tgggtcgtga tcgactgcgc cgtgcttgac tcgaacggct
      901 cggccgacgt cgggtacagc ttggccacct gctcgtcggt catctgggcg agcagcgcct
      961 tgccgagcga ggtgcagtgg gcaggggcca acgtccccgt ccgtgccacc acacgcaggg
     1021 cgcgttcgga ctccaggccg tcgacgtagc gcaccttgtt gccctccagc gcaccgagat
     1081 ggaccgtctc accggtctcg gcggccagct cctccatcgc gggccgagct tgctcgcgaa
     1141 cattcagcga gccgacgacg gccaggccca cctcgaccag tgcgggaccc gcgatgtagg
     1201 cgcgcgtgac cggttcttgc tggacgaagc cgtggtgggc aagcatcgcg agcagccggt
     1261 gcgccgtcga gtgcgcgact cctaggtact tgcacgcgtc ggtgagccgg acccttggct
     1321 gctcacggaa cagcagcagc aagagcagcg cgttgttgac cgaggcgatc gggtaggcgg
     1381 ggccggtttc gctctccggt cgcgtcttct gcatagaaga atagtaccgt ctgccccggt
     1441 tggtgttcgc cgacgcgacg acacagcagt gcgctgcaaa gtgacgcttg acacacccgc
     1501 ggccgggcca taggttttct cacagcagaa gatgtttcca tcttgaagac atccccaaga
     1561 ggtctgcatg gagcaagaga tcgttcggcg ccccgacgag gacacccccg acgaggacgc
     1621 cgccgaagag ctcgccgccg aaccgagccc ggctgacggc gaccagaatc gggacgcccg
     1681 gctgacccga cgagttcacg tcgtcctcgg cctggtgatc gccgcgctcg gggcgtggct
     1741 gctgtacaag agccggaccg agctggagtt ccgcggtccg aacaacgagc caggcccggg
     1801 ctaccttccc gtcctgctga ccatctgcct catcgccctc ggcctcgcgc tgtcggcggt
     1861 gtggttgtgg ggtccgaagg cgcgccgcgg tgaggcgccc acgctgtcac tgtctcccca
     1921 gggcatcggc cgcgcgctgc tggtatggct cgccctgttg gtctgcgcgt tcctgatcga
     1981 gccgctgggc tttctggcgg ccggtgaagt actggtcgtg ctggtgatcg tggtgatcga
     2041 gcgaatccga tccatcccat tgatcatcgc gctgctgttg ctgccgcctg ccatgtactt
     2101 gctgttcgac accctgctcg aggtgcaact cccgggagga accctgtggt actgaatcag
     2161 actgggcgcc aacggtgaac gcggtcgagg gcctgctaca cgggttgcaa tcggcgatca
     2221 cgccgacgaa tctgttgtgg ctgctcgtgg gctgcctgct tggcaccctc gtcgggatcc
     2281 tgcccggcct cgggccaccc gccacgatcg cgattctgtt gccactcgcc accggcttcg
     2341 cccccgcgac cggtctgatc atgatggccg gcatctacta cggcgcgaag tacggcggat
     2401 cgaccacgtc gatcctgttg aacatccccg gcgagtcgtc gtcggtggtg acttgtatcg
     2461 acggctacga gatggccaaa cagggccgcg cggggaaagc cctaggcatc gcggcgatcg
     2521 ggtcgttcgt cgcgggcacc cttggcgtga tcggcctgac gttcctggcg cccgcggccg
     2581 ctgacgtggc ggtcaacttc gggccgcccg agtactcggc gctgatggtg ttcgcgctgc
     2641 tgctggtgat catgctggcg ggcgattcga tgctcaaggg cttcatcgcg atgttcatcg
     2701 ggctcttcct gtccaccatc ggcaccgacc tgttctccgg cgcacaacgg ttcaccggcg
     2761 accagatcga gctcgcaggc ggcatcgagt tcatcgccct gtccgtcggc atcttcgcgg
     2821 tcggcgaggt gctgatcaac atcgaagaca agaccgagaa gcaactcttc caggtgccca
     2881 acaagattcg cgatctgctc ccgtctcgac aggacatcaa gaagtcgacg cctgccatgc
     2941 tgcagggcgg cattgtgggc ttcctgatcg gcatcctgcc tggtgcaggc tcgaccgtcg
     3001 cgtcgttcgt ctcctacatc atcgccaaga agacatcgaa gacgccggag gagttcggca
     3061 agggctcgat cgagggtgtc gcggctccgg aggcggcgaa caactcggaa accggcggcg
     3121 cgatggtgcc gctgctgacc atgggcattc ccggatctgg cacgggcgca gtacttctcg
     3181 gtgcattggt gctctatggt ctcaaccccg gaccgttgct cttccatgag cacgccgacg
     3241 tcgtgtggcc gatcatcgcc agcatgtacc tgggcaacat cgccctgctg gtgatgaacc
     3301 tgccgatggt gccgctgttc gcgaagctgc tgaacacccc ttataaagtt ctctacccgg
     3361 cgatcctttt gatctcggtc gtcggcgtct acagcgtgaa cttctcgatc ttcgatgtcg
     3421 gcatgctggt ggtctttggg ctcctcggct acgtgatgcg caagctcgac atcccgcctg
     3481 cccctttggt tctcgccttc gtgctaggcc cgatcgcgga aaatgcgatc cggcaatcat
     3541 tgttgctgtc gaacaacagc ccgatcatct acctggagcg gcccatctcg gcgacgctga
     3601 tcggcctggc ggtcgcgctg ctcctggtgt tggcgttcgg tcgcggtcgc aaggtccgcg
     3661 atcaggtcgt ggagatcgac gcatgaaccc agtcctccga aggaaggaag cggacatgtt
     3721 ccgaatcagg caactactca ccgtcgccat tgcggtgctg gggctgatcg ccctcgccgg
     3781 atgcggcagc tcgggcggtg gcggtaagtc cggtcccgtc gaggtggtca cgcacaacgc
     3841 cgtcggcggc ggcagcgacg tgttcacccg ccagatgatc aaggtgatgt acgagaacaa
     3901 gatcatcgac accaactggc cggtccgcaa tgtcccggcc ggggactcga tcggcgcgat
     3961 gtcgtacatg ttcgaccaca agggcaatgc ggggttgatc gcacaggtga cgccgacgtg
     4021 gctggcgacc ccgatgaccg tcgagaacgc gaaggtgagc cttgctgggc tgaccccaat
     4081 cacgttggtc gccaccgaac ctcaggtcgt ggcgacgaat gccaaatcgc aattcaacac
     4141 gttcgccgac ttcgtggccg ccgcgaagaa ggccccggac accctcgtcc aagccggtgg
     4201 ttccagcacc gccaacgacg cgctcacccg gctcgtgttg caagacagcc tcggcgcgaa
     4261 gtggaagttc ctgtcgttcg aggacaccgg atcgcggatc accgccctgc tgcgcgacga
     4321 cgcaaacatc atgctgggca gcgcgtccga cgtcgcagag caggtgaagg ccggacagct
     4381 caaagtgatt gcagtcattg gcaatcagcg gctggatgcc ttccccgatg tcagcaccac
     4441 cgaggagcag ggcatcgatt cgtccaaggt gcccgtgcag ttccgtgccg tgatgggtgc
     4501 gcccgacatg ccggcagacg ccgtgcagaa gtaccaggac gatctgtcga agatggtcga
     4561 aaccgacgac tggaagaacc tcatcaagga cgacggcctg gtgacccaga acctccagga
     4621 cgacaagtta gagacgtatc tgaaggaaca gactgacatc gtcggcggcc tgctgcaggg
     4681 acttggcctg aggaaggatc agtgacgagc gccggtccga catcgaccct tgcgacgttc
     4741 gtcgccgagc aacagagcga gcaggtaccc gaacccgtca tgcacgcggc ccggcgcgcg
     4801 cttgtcgacc acatcggcgt gacggtcgca ggctcgacag acgacgccac ccggcgggcg
     4861 cgcgacgcca tcgccgccag cgaaagcggt gggggtgcaa cggttgtcgg ggcgcggatg
     4921 cgagtctcac cgcccttcgc ggccttcctc aacgcgttcg cgtcacacgt gctcgatctc
     4981 gacgacgtgt acaacccgcc gggcacaaca gtgcacggca gctgctcggt gtggccggcg
     5041 atcctggcca tcgccgacaa ccggccggtc agcggtcgtg acgccctggc cagtttcgcg
     5101 ctgggattcg aggtggagac gcgggtcgca cacgccgctg gccaaacgca ttacgacgtc
     5161 ggctggcacg tgacaggaac atccggtcat gtcggggcgg ccgctgcggc cgcgcgcacc
     5221 atgggcctgg accgcaacca gatcgagcac gccatggccg ccggggccac ccaggccgcc
     5281 ggattgcgga tcatggcggg cagcgatctc aagagcatgc accccggcaa ggccgcgatg
     5341 gacggcgtgc tcgccgccac cctggtcgag caccagctga cagccagtgc caaggcgatc
     5401 gaaggcgact tcggctatct cgcggtgatg agtgccgcgc ctgcacccga gaagatcacc
     5461 gcggggttgg gcgagagttg gaacctgaca tcgaacgggc acaagctcta tccgagcggc
     5521 tccctgaccc atcccatggt cgacggcgtg atcgcgttgg tcaccgaaaa tggcatcgcc
     5581 ccagacgatg tcaccgtcat agacgtgcga gtctcgccgc cggcggcacg gttcaccgac
     5641 cggccccgcc cggccacacc gatggaggcc aagttcagcc tgcggcactg cgcggccgcg
     5701 gcggtggtgt tccgccggct gggcaccgac gaactttccg ccgaggtgct ggcgcggccc
     5761 gacgtgatcg acctgcgcga ccgggtgaag gtcgttgcgg atcccgcgat cggcaagcag
     5821 gacgccgacg tcgagatcac gctcgcgtcg ggacggacgc tgtccaccga ggtgcgcggc
     5881 aaccgcggca cgccgtcggc gccgctcgcc gacgacgaac tcaacgtgaa gtttctcgag
     5941 ttggtcgcac ccgtgctggg cgcagagcgc gccgagcggc tgctggcgac gtgctggcag
     6001 ctcgacgagc ttcccgacgt ctccgtgctg ctgcagcaga ccgtcccggc ggccgatggc
     6061 tgacattgcg ctcgtcactg gcgcaggcag cggtctgggt gcggtgatcg ccgaacgtct
     6121 tgcgcgcgaa ggacttacgg ttgcggtgaa cacccgggcc cacaccgaag aggccgacgc
     6181 ggtcgccgcg gccatccgcg caggaggtgg cagcgccttc gccgtcaccg ccaacgtcac
     6241 cgatccatcg gcagtgcggc gcatgttcga gaccgtggcc gcgcacggca cgctgcgtgt
     6301 cctcgtcaac aacgccgcct accgtccacg gcagcgagtc caggcgatca ccgaggacga
     6361 ctggcatcag gttcgctcgg tcacgcttga cggcgccttc cgctgcatcc ggtgcgcgtt
     6421 gcctgcgctc atcccgggtg ggcggatcat caatatcctt ggccgcaacg cgctggcagg
     6481 cgatcctgag cgcgtgcacc tctcggcggc caagcacgga ctgctcggcc tcacgctggc
     6541 actcgccaag gcgctgcgcg acgacggggt cgcggtcaac gcggtgtcgc cgggtgtcga
     6601 taccgatggt cccgagctgg accggtgccg ggccgagatc gcttctcagg tagcgagatt
     6661 ggccttcgtg gacgccaaag agctcaccgg caccgtcgtc cgcgtcgact gcgacggatc
     6721 cgtcgtcagc gtcccgacgg cgtggtgagc gccagcatcg cctcgacgtc gccgtcagct
     6781 cgcagcgcaa agacgctgtc ggccaaccgt tccgcgcgat cgcgaccgag cacaccgtcg
     6841 gtgaggccat ggaacttctc ccgcagcccg tcgtcggtca gcctgttgcc gggtgtgccg
     6901 cgcgcgtgat cgacccacgt ctccaccgtc tcaccgtcgt cggtgagcgc cgtcgccccg
     6961 gcctgtgtgt gcgggacgtc ggcgtccggg acgacctcga tgcgcgacat caggtcgcgg
     7021 acgtctgcac gcacgacggc ggcgtcggaa aactcggcct cacccgcccg gccgtcgagc
     7081 agcgcgatgg aacacgcgaa cgtaaccgag aacttgcctt ccaaacccgt acggggatcg
     7141 gtctttccgg tcagctcgag caccagcggg tgcacccgca gccggatctt ttcgatgcgc
     7201 cccacggtga gcccgcgacg cacggcgagg tcgcgaaccg cgtcgatggc cggatggatc
     7261 accacgccgc acgcgtaggg cttcaccccg ttgtcgaaga tctgccaacg ctgcccgagc
     7321 ccgtcgacaa gatcggccgg cgtgctcgcc gacgacatca ccgcgaacat tccgcggcgg
     7381 ccctgcaacg ggtcgggtgc gccggtgaat cctcctgccg caagaaggcc cgcccagacg
     7441 cctgccgcgg cggcatgacc ggcgtggaac ggcttggtca tggtgccgaa ctgttcgcga
     7501 tggcctgctg cctgagtcgc cgcgatgctc aggcaatggg tcgctgtcgt tcccgtcagc
     7561 cccagcagcc tgatcgcaac ggtcgccgag gcgagcgctc cggtggtgcc ggtcatgtgc
     7621 cagccggcgt catagtgctc gggataaagc gcattactgg cgcgcgcggc gagctcgtag
     7681 cccagcgcat gcgccgcgag cagatcggcc cccttggcgc cccgccattg cgccagcggc
     7741 gtgccagagg cgtacagcgg cgtcgtcgga tgcaggatcg tgggtacgtg cgtgtcatcg
     7801 aaatccagtg catgacaaga gattccgttg accagggctg cgtcggtgac ccgcagccgg
     7861 tgaggcgtgc cgagcacggt ggcctgcgga tcaccgccca gcgcctggca ttgctccctg
     7921 gctatccgtg cggcgggttc ttcggcgccg gcgacggcga ggccgacatg atcgagcaga
     7981 caccgggtgc tctcgtgcag cacctcggcg gggatgtcgg aggcggcggt gtggcacacg
     8041 aagtcgagca gcgtgcgcgt ctccgccgag gtccgatcgt cgtagctttg cgaacgcgat
     8101 cccatgtagt ctgtataaca gaatttcttt ccgtattaca gacgggagtc tgtggtgggt
     8161 gatcgaaggt ccgtgagcgg cgcgctggcc gccttcgctg ccggtatccg ggtcgagaat
     8221 ctccccgacg acgtcgtgca tcaggccgtc cgctgcctcg tcgactggct cggctgcacc
     8281 atcgccggcg ccgcaacgcc cgagagcgcc cgggtccggg ccgcgatccg cgcgatggag
     8341 cccgacggat cacgtgctgc aggcatcgtc ggcaccctcg accgcggctc g
//
LOCUS       DASRJA010000330         6945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2887147,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000330 DASRJA010000000
VERSION     DASRJA010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2887147"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFC01_14515"
     CDS             complement(<1..200)
                     /locus_tag="VFC01_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80879.1"
                     /translation="MLEFAIIIVLIGAMLALAAPWIMRRRGAGADWVQGQLLVTGVSP
                     KPDATGEQFVTITGVINGPTVN"
     gene            224..1006
                     /gene="hisN"
                     /locus_tag="VFC01_14520"
     CDS             224..1006
                     /gene="hisN"
                     /locus_tag="VFC01_14520"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HZN80880.1"
                     /translation="MTEVREDVALALQLADQADALTTERFGALDLHIDTKPDLTPVTD
                     ADRGAETMLREILAEQRPDDVVLGEEFGGTAVFTGRQWVLDPIDGTKNYVRGVPVWAT
                     LIALLVDGIPTVGVVSAPALHRRWWAGTGEGAFTSFAGVERRISVSGVNDVASASLTY
                     SDLTTGWGELRSRFVALTDAVWRVRGYGDFWSYCLVAEGAVDIAVEPEVKLWDLAPLD
                     ILVREAGGAFSSLDGSPGPHGGSAVATNGLLHEAVLNRLNGA"
     gene            1027..2106
                     /gene="amrS"
                     /locus_tag="VFC01_14525"
     CDS             1027..2106
                     /gene="amrS"
                     /locus_tag="VFC01_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003913437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZN80881.1"
                     /translation="MTFDSFTVPTSHWHGLEDGRIQCDVCPRACKLREGQRGLCFVRA
                     RADDQIVLTSYGRSSGFCVDPVEKKPLNHFLPGSAVLSFGTAGCNLACKFCQNWDISK
                     SRETDTLADRATPQRLARVADEMGCSSVAFTYNDPTIFWEYAADVADACRERGIKAIS
                     VTAGYMCPQPRAEFYRHIDAANVDLKAFTEEFYHRVCVGHLADVLDTLVYLRHETDVW
                     VEITTLLIPGLNDSDAEIIAECAWIRDNLGVDVPLHFTAFHPDYKMLDIPPTPPETLT
                     RARRIGLAEGLRFVYTGNVHDRDGDTTRCPGCGDAVVVRDWYAMRHYSLTDSGRCERC
                     GTQVPGVFDGPVGTWGARRLPILVS"
     gene            complement(2116..2499)
                     /locus_tag="VFC01_14530"
     CDS             complement(2116..2499)
                     /locus_tag="VFC01_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012832345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HZN80882.1"
                     /translation="MIEYLDLDDLLDIAREAVGNDAAIGDYGLLESARARPGASVFGQ
                     DAYADLHLKAAALLHSLARNDALVDGNKRLAWTACRTFLAINGKWISAPEDDRFEFVI
                     RVATGALPDLDEIGRQLRAWSYQER"
     gene            complement(2496..2702)
                     /locus_tag="VFC01_14535"
     CDS             complement(2496..2702)
                     /locus_tag="VFC01_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HZN80883.1"
                     /translation="MGMTLRTSAQQTEALRQQAAAEGRSMQAVALSAIDEYIERRAHK
                     TKVAAALQRVVREEAAVLERLKDA"
     gene            2859..4235
                     /locus_tag="VFC01_14540"
     CDS             2859..4235
                     /locus_tag="VFC01_14540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80884.1"
                     /translation="MTNTLPSKARSGRESAVGLQKHKRTATDIGLALITPIVGQEFLD
                     KYNLRDPLNRGLKYGVKQVFSAAGAANRQFKRVSGGPSGPTRLKKSGADYFDLTPDDE
                     QKMIVDTVTEFAAEVIRPAAHDADEAATYPEDLVGKATELGITAINVPEDFEGIAAHR
                     AAVTNVLVAEALAYGDMGLALPILAPAGVASALTHWGSADQQATYLKDFAGENVPQAC
                     VAIAEPQPLFDPTALKTTAVRTPSGYRLDGVKSLVPAAANAELFIIAAQLNGRPALFI
                     VESSAKGLTVTQDPSMGIRAAALGRVELDRVTVPLSARLGEEDATDEDYSEAIALSRL
                     GWAALAVGTAHAVLDYVVPYVKERHAFGEPIAHRQAVAFMCANIAIEFDGLRLITWRG
                     ASRADQGLPFAREAALAKRLGTDKGMQIGLDGVQLLGGHGYTKEHPVERWYRDLRAIG
                     VAEGVVVI"
     gene            4257..5471
                     /locus_tag="VFC01_14545"
     CDS             4257..5471
                     /locus_tag="VFC01_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN80885.1"
                     /translation="MAINLELPRKLNAVIEKAHQGAAEMLRPISRKYDLREHDYPAEL
                     ETLATLFEGISEANAFAFAGAEAFRDGNEPKDANHNGGNMSAVLNVMEISWGDVGLML
                     SVPRQGLGNAAISGVATDEQLELLGKDVWAAMAITEPGFGSDSAAVSTTAKLEGDEYV
                     INGEKIFVTAGSRATHIVVWATLDKSLGRAAIKSFIVPREHPGVTVERLEDKLGIKAS
                     DTAVIRFDNARIPKDNLLGSPEIHVEKGFAGVMETFDNTRPVVAAMAVGIARAALEEL
                     RKILTDAGVEISYDKPAHAQSAPAAEFLRMEADWEAGYLLTVRSAWQADNDIPNSKEA
                     SMGKAKAARVASDITLKAVEMAGTTGYSEEALLEKWARDSKILDIFEGTQQIQQLVVA
                     RRLLGLSSSQLK"
     gene            complement(5537..6535)
                     /locus_tag="VFC01_14550"
     CDS             complement(5537..6535)
                     /locus_tag="VFC01_14550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HZN80886.1"
                     /translation="MVAVNALVAHQDAEGSIALKHEVVDESFLPEGDVQIAVEYSGVN
                     YKDALAITPKGGVARSYPLIPGIDVAGTVTASSAADFAVGDRVVAHGYDIGTGRHGGY
                     ADTARYPADYLVKLDALTTAQAAAIGTAGFTAAMSVNAIRAHGVRPQDGPVLVTGATG
                     GVGSVSVDLLAGLGYEVIASTGKAEAHDYLMGLGATEVIGRLPAEGEKIRPLGKSAYA
                     AVVDSVGGDTLAYALSVLNYAGVAAISGLARSADLPTTVMPFILRGVTLAGIDSVLLR
                     IEKRRELWQSIASDLMPRHLDDITHDISILEAPETLRTIVGGGVTGRTRVVVKDGF"
     gene            complement(6607..>6945)
                     /locus_tag="VFC01_14555"
     CDS             complement(6607..>6945)
                     /locus_tag="VFC01_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZN80887.1"
                     /translation="YKANDMWGPTEDPNSAWKRNDPMIQIPALVANNTRLWVYCGNGT
                     PNELGGADLPATFLEGLTIRTNETFRDNYIAAGGKNAVFNFPPNGTHNWAYWGAQLQQ
                     MKPDLISYLG"
BASE COUNT         1230 a         2289 c         2235 g         1191 t
ORIGIN      
        1 tcattgactg tcgggccatt gatgacaccg gtgatcgtga cgaattgctc gccggtggca
       61 tccggtttcg ggctgacccc ggtgaccagc aactggccct gcacccaatc cgcaccggcg
      121 ccgcgacgcc gcatgatcca gggcgccgcg agcgcgagca tggccccgat aagcacgatg
      181 atgatcgcga actccagcac accgccatgg taggactgct gccatgacag aggtccggga
      241 agacgttgcg cttgccctgc aattggccga ccaggccgac gcgttgacga cggaacgctt
      301 cggcgcgctg gaccttcaca tcgacaccaa gccggacctc accccggtga ccgacgcgga
      361 tcgcggggcg gagacgatgc ttcgcgagat attggccgag cagcggccgg acgatgtggt
      421 gctcggcgaa gagtttggtg gcacagccgt tttcactggc cgccagtggg ttctcgatcc
      481 gatcgacggc accaagaatt acgtccgcgg cgtgccagtc tgggcgacgt tgatcgcgtt
      541 gctggtcgac ggaattccca ctgtgggagt ggtgagcgca cccgctttgc atcgcaggtg
      601 gtgggcgggc acgggcgagg gggcattcac gtcgttcgcc ggcgtcgaac ggcgcatctc
      661 ggtgtcaggc gtcaacgatg tggcgtcagc gagcctgacg tactccgacc tcaccaccgg
      721 ctggggtgaa ttacggtcgc gcttcgtggc cctgaccgac gcggtgtggc gggtgcgtgg
      781 ctacggcgac ttctggtcct actgcctggt tgccgagggc gccgtcgaca tcgcagtgga
      841 gcccgaggtg aaactgtggg acctggcgcc gctcgacatt ctggtgcgtg aggcgggcgg
      901 ggcgttcagc agcctcgatg gcagccccgg accgcatggc ggcagtgccg tcgccaccaa
      961 cgggctactg cacgaagccg ttctgaacag gctcaacggg gcgtagcctg gcgatcggag
     1021 ggtgtcatga ccttcgactc tttcaccgtg ccgactagcc actggcatgg cctcgaagac
     1081 ggccggatcc agtgcgacgt gtgcccgcgc gcctgcaaac tgcgggaggg tcagcgcggc
     1141 ttgtgcttcg tccgcgcccg cgccgacgac cagatcgtgc tcaccagcta tggccgatcc
     1201 agtgggttct gcgtcgatcc ggtcgagaag aagccgctca accacttcct gccgggctca
     1261 gcggtgctgt cgttcggcac tgccggttgc aacctcgcgt gcaagttctg ccagaactgg
     1321 gacatctcga agtcgcggga gaccgacacg ctcgccgacc gcgcgacgcc ccagcggctg
     1381 gcacgggtgg ccgacgaaat gggctgtagc agtgtggctt tcacgtacaa cgatccgaca
     1441 atcttctggg agtacgccgc cgacgtcgcg gacgcctgcc gtgaacgcgg catcaaggcg
     1501 atttcggtga cggcgggcta catgtgcccg cagccgcgtg ccgagttcta tcggcacatc
     1561 gacgcggcca acgtcgacct gaaggcgttc accgaggagt tctatcaccg ggtctgcgtc
     1621 ggccatctgg ccgacgtgct cgacaccctc gtgtacctgc ggcacgaaac cgacgtgtgg
     1681 gtggagatca ccacactgct gattcctggg ctcaacgaca gcgatgccga aatcatcgcg
     1741 gagtgcgcgt ggattcggga caacctcggc gtggatgtgc cgttgcattt caccgccttt
     1801 caccccgact acaagatgtt ggacatcccg ccgacgccgc ccgagacgct gacccgggcc
     1861 cggcgaatcg ggctcgccga aggattgcgg ttcgtctaca ccggcaatgt tcacgaccgc
     1921 gacggcgaca ccacgcgctg tcccggctgc ggcgatgccg ttgtggttcg cgactggtat
     1981 gcgatgcggc actacagcct gacggactct ggccgctgcg agcgatgcgg cacgcaggtg
     2041 ccgggcgtgt tcgacggccc cgttgggacg tggggcgctc gacgattgcc cattctggtt
     2101 tcgtgagttt tcgactcagc gctcctgata gctccaggcg cgcaattgcc tgccaatctc
     2161 gtcgagatcg ggcagagcac ccgtggcgac ccggatgacg aattcgaagc gatcatcctc
     2221 gggggcgctg atccacttgc cgtttatggc caggaaggtt cggcacgccg tccacgccag
     2281 tcgcttgttg ccgtccacca gcgcgtcgtt ccgcgcgagg gagtgcagta gtgcagcggc
     2341 cttgaggtgc agatctgcgt aggcatcctg accgaacacc gacgcgcccg ggcgtgcccg
     2401 agctgattca agcaagccgt aatccccgat cgcggcgtca ttgccgacgg cttcccgagc
     2461 gatgtcgagc aggtcatcga ggtccaggta ctcgatcacg cgtccttgag gcgctccagc
     2521 accgctgcct cttcgcggac cacgcgctgc agcgccgcgg cgaccttggt cttgtgcgcc
     2581 cgccgttcga tgtactcatc gatggcagac agcgcgacag cctgcatcga ccgcccctcg
     2641 gcggcggcct gctggcgcaa cgcttcggtc tgctgtgcgc tggttcgtag agtcatcccc
     2701 atgaccgaga tgataccgaa ctgataccac tcggtcccta cggcgttgtc ccgcccgctg
     2761 tgaattgcac tacaacttac tcaagagtca gttcgcatac cttactctgg agtcagttgc
     2821 gtattcgtgc acgtctaccc gcaagcgagg cagctgaaat gaccaacacc ctgccgtcca
     2881 aggcgcgctc cggccgcgag agcgccgtcg gcctgcagaa gcacaagcgg acagcgaccg
     2941 acatcgggtt ggcgctgatc acgccgatcg tcggccagga attcttggac aagtacaacc
     3001 tgcgcgaccc gctgaaccgg ggcctgaaat acggcgtaaa gcaagtgttc tccgccgcgg
     3061 gtgcggcgaa tcgtcagttc aagcgcgtgt cgggcggccc gagcggcccc acccggctga
     3121 agaagagcgg cgccgactac ttcgatctga cccccgacga cgagcagaag atgatcgtcg
     3181 acacggtcac cgaattcgcg gccgaggtga tccgacccgc cgcccacgac gccgacgaag
     3241 ccgcgaccta ccccgaagat ctcgtcggca aggccaccga actgggcatc accgccatca
     3301 atgtgccgga ggacttcgag ggcatcgcgg cccatcgcgc cgccgtgacc aacgtgttgg
     3361 tggccgaggc actggcctac ggcgacatgg gtctggcgct gccgatcctc gcgcctgcgg
     3421 gggtcgcatc cgcactgaca cattggggca gcgctgacca gcaggccacc tacctgaagg
     3481 acttcgccgg cgaaaacgtg ccccaggctt gtgtcgcgat cgccgaacca caaccattgt
     3541 tcgatccgac cgcattgaag accaccgcgg tgcgcacgcc cagcggctac cgcctcgacg
     3601 gcgtcaagtc tctggtcccc gcggctgcga atgccgaatt gttcatcatc gcagcacaac
     3661 tcaacggcag gcccgcactg ttcatcgtcg aatcctcggc gaaaggcctc acggtcacgc
     3721 aggacccgag tatgggtatc cgcgcggcgg cgctcggccg ggtcgagctt gaccgcgtca
     3781 cggtgccgct gtcggcgcga ctcggtgagg aagacgccac cgacgaggac tactccgagg
     3841 ccatcgcgct gtctcggctt ggctgggcgg cgcttgcggt cggaaccgcg catgcagtgc
     3901 tcgactacgt ggtgccatat gtcaaggaac gccacgcatt tggtgagccg atcgcccacc
     3961 ggcaagcggt tgcattcatg tgcgccaaca tcgcgatcga attcgacggg ctgcggctga
     4021 tcacgtggcg cggagcgtct cgcgccgatc agggcctgcc gttcgcccga gaggcggcac
     4081 tggccaagcg actcggcact gacaaaggca tgcagatcgg cctggacggc gtccagctgc
     4141 tcggcggcca cggttacacg aaggaacacc cggtcgaacg ctggtaccgc gatctgcggg
     4201 caatcggcgt cgccgagggc gtcgtcgtca tctaactgaa gatcggaagt cgagccatgg
     4261 caatcaattt ggaactcccc cgcaagctga acgcggtcat cgagaaggca caccagggcg
     4321 ccgccgagat gctgcgccca atctcacgca agtacgacct gcgcgagcac gactaccccg
     4381 ccgagctgga gacgctggcc accctcttcg aaggcatttc ggaggcaaac gcattcgcgt
     4441 tcgccggcgc cgaagccttc cgggacggga acgagcccaa ggatgccaac cataacggcg
     4501 gcaatatgtc ggccgtgctc aacgtgatgg aaatcagttg gggcgacgtc ggattgatgc
     4561 tgtcggtgcc ccgtcaggga ctcggcaacg ccgccatttc cggcgtggcc accgacgaac
     4621 agctggagct cctgggcaag gacgtctggg ccgcgatggc gatcaccgaa cccggcttcg
     4681 gctccgactc ggcggcagta tcgacaaccg ccaagctcga aggcgacgag tatgtgatca
     4741 acggcgagaa gatcttcgtc accgccggct cgcgcgccac ccacatcgtg gtgtgggcca
     4801 ccctcgacaa atcgcttggg cgcgcggcca tcaagtcgtt catcgtgccg cgtgaacatc
     4861 ctggggtgac cgtcgagcgc ctcgaagaca aactcggcat caaagcctcc gacaccgccg
     4921 tcattcggtt cgacaacgct cggattccaa aggacaacct gctcggcagc ccagaaatcc
     4981 acgtggagaa gggctttgcc ggggttatgg agaccttcga caacacccga cccgtcgtcg
     5041 ccgcgatggc tgtgggcatt gcccgcgccg cactcgagga actgcgcaag atcctcaccg
     5101 acgcgggcgt cgagatctcc tatgacaagc ccgcgcacgc gcagagcgct cccgccgcgg
     5161 aattcctgcg gatggaagcc gactgggaag ccggttacct actcacggtg cggtccgcgt
     5221 ggcaagccga caacgacatt ccgaactcca aggaagcgtc gatgggcaag gccaaggccg
     5281 cccgggttgc cagcgacatc accctcaagg ccgtcgaaat ggcaggcacc acaggctatt
     5341 ccgaggaagc cctgctggag aagtgggccc gcgacagcaa gatcctcgac atcttcgagg
     5401 gtacgcagca gattcagcag ctggtggtgg cccgtcgcct gctgggcctg tcgtcgtccc
     5461 agctcaagta gtccccagtc cccagtcccc agcttgcgcg tgggcccatg cgcgtgggcc
     5521 catcgcgtgg gcccacttag aaaccgtcct tcaccaccac gcgggtgcgg ccggtgacgc
     5581 cgccgccgac gatggtgcgc agcgtctcgg gcgcctcgag gatcgagatg tcgtgcgtga
     5641 tgtcgtcgag gtgacgcggc atcaggtcgg acgcgatcga ctgccacagc tcgcgacgct
     5701 tctcgatccg cagcagcacc gaatcgatgc cagctagggt gacgccccgc aggatgaacg
     5761 gcattacggt cgtcggcagg tcggccgacc gcgctaagcc tgaaatcgcg gccacgccag
     5821 cgtaattcaa cacgctcaac gcgtaggcca gcgtgtcacc gccgacgctg tcgacgaccg
     5881 cggcataggc tgacttgccc agcgggcgga tcttctcgcc ctcggcgggc aacctgccga
     5941 tcacctcggt cgcgccgaga cccatcaggt agtcgtgtgc ctcggccttc ccggttgatg
     6001 cgatcacctc gtatccgaga ccggccaaca agtcgacgct gacactgccc acgccaccgg
     6061 tcgcgccggt taccagcact gggccgtcct gcgggcggac accgtgcgct cgaattgcgt
     6121 tgacgctcat ggccgcggtg aaccccgcgg tgccgatggc tgccgcctgt gcggttgtca
     6181 gcgcgtcgag cttgaccaaa tagtcggccg gatagcgcgc ggtgtcggca tatccgccgt
     6241 gccgaccggt tccgatgtcg tatccgtgag cgacgacgcg gtcaccgacc gcgaagtcgg
     6301 cggccgagct ggcggtcacc gtgcccgcga cgtcgatacc ggggatgagc ggataggacc
     6361 gtgccacacc acccttcggt gtgatggcca gggcgtcctt atagttgacg ccggaatact
     6421 ccacggcgat ctgcacatcc ccctcgggga ggaagctttc gtcaacgacc tcgtgtttga
     6481 gggcgatcga cccttccgcg tcctgatgtg cgaccaaggc attgactgcg accatgcggc
     6541 tcagtattca ccaaacgcga aagcccccga cgggtgtgtc gggggccttc gcgtcttctg
     6601 gtccgcttag ccgaggtacg agatcagatc cggcttcatc tgctgaagct gggcccccca
     6661 gtaggcccag ttgtgggtgc cgttgggcgg gaagttgaac acggcgttct tgccgcccgc
     6721 ggcgatgtaa ttgtcgcgga acgtctcgtt ggtccggatc gtcaggccct cgaggaacgt
     6781 cgcaggcagg tcggcgccac ccaactcgtt gggtgtgccg ttgccgcagt acacccacag
     6841 tcgcgtgttg ttcgccacca acgccgggat ctggatcatc gggtcattgc gcttccaggc
     6901 gctgttcggg tcctcggtcg ggccccacat gtcgttcgcc ttgta
//
LOCUS       DASRJA010000331         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2261406,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000331 DASRJA010000000
VERSION     DASRJA010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2261406"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..1240)
                     /locus_tag="VFC01_14560"
     CDS             complement(347..1240)
                     /locus_tag="VFC01_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycobacterial-type methylenetetrahydrofolate
                     reductase"
                     /protein_id="HZN80888.1"
                     /translation="MTLNTVAFELVPPNADLGPEQAVEEARKVLRFSKETGIDGQLGH
                     VMIPGMIEEDDDRPVEMKPRLDVLEFWNIIKPELPGINGLCTQVTAFMDESSLRHRLT
                     DLRDAGFDGIAFVGVPRTMNDGEGAGVAPTDALSIYDQLVPNRGAILIPTREGEHGRF
                     NFKCNQGATYGMTQLLYSDAIVGFLTEFAKAGDHRPEILLSFGFVPKVETRIGLINWL
                     IQDPGNAAVADEQAFVTQLAETDPAQKRKLMIDLYKRVVDGVADLGFPLSIHLEAAYG
                     ASAPAFETFAEMLDYWSPAKG"
     gene            1527..1739
                     /locus_tag="VFC01_14565"
     CDS             1527..1739
                     /locus_tag="VFC01_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80889.1"
                     /translation="MKKITFATVVSSALAALAIGLAGAALADAGSGSNVPAPAPVYPQ
                     TATGGANPYTPYGVDPYVPFGVWSQH"
     gene            complement(1743..2303)
                     /locus_tag="VFC01_14570"
     CDS             complement(1743..2303)
                     /locus_tag="VFC01_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80890.1"
                     /translation="MLSIVPYPKRFTAAALAAVFVATCAACGSTTTRSAGPISHVNVD
                     CGLQTPAPSGFAELPGFTVTEICPADVDPAFVTHEFGGLAAGVVSQNGNPILKVLVGQ
                     LKSGSGDTFVHTYVGKLSDQTREDVGVPSEPAEVDGHVLTHFNIPLQTDGYAYADGDT
                     VVIAYVVPFPPPATVEAALSAILGNV"
     gene            2307..2816
                     /locus_tag="VFC01_14575"
     CDS             2307..2816
                     /locus_tag="VFC01_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80891.1"
                     /translation="MCVLKDSWRPTVGCLATLTMLTACDAGGSDAPPTSLASPTSAAA
                     TTTSTTAQGARTQKWIDLDVGDCLADPPPTDPSVVTVSVVDCAIPHAAEVYLRTNVEV
                     NAAIADVADQQCGAGLPEYTGQSGGPFVVTYLIDSNQDRTSGNPLPSTVICLLQAPGG
                     GPLTGSARR"
     gene            2923..>3167
                     /locus_tag="VFC01_14580"
     CDS             2923..>3167
                     /locus_tag="VFC01_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80892.1"
                     /translation="MSVASDATVVLDDRFARELPEMAVRWQAETPPDPQLLVLNEPLA
                     ASLGLDPAWLRSPDGLRFLVGSLVPNGATPVAQAYAG"
BASE COUNT          576 a         1033 c         1000 g          558 t
ORIGIN      
        1 cgcttccaca ccaaccttga cctgcgctga cgccgcaaca acggcactga cccgatccac
       61 catcgccgac aacgccgaac gcgtcatcgc gaactcaacc ggaccgaaca catgttttcc
      121 gaccgccatc cgtgaccaac aacatcgccg aggtcttgcc gacatcgacg gcaacgacga
      181 tcgtggacga cgtgactagc gcaccatgat gcacagacac aacaacacct ccgggggcac
      241 cccaggagaa ccaaagcgcg ccaacgcttt gatcctcaaa gggaaatctg acaatccctt
      301 cctgacaccc ccggaggcgt ttgtgaagtg actacctcaa ctcatatcag cccttggcgg
      361 gcgaccagta atcgagcatc tccgcgaagg tttcgaaggc cggcgccgat gcgccgtatg
      421 ccgcttcgag atggatgctc agcgggaatc caaggtcggc gacgccgtcg accacccgct
      481 tgtacagatc gatcatcagc ttgcgcttct gcgcgggatc ggtttccgcc agctgcgtga
      541 cgaacgcctg ctcatcggcg acggccgcgt tgccggggtc ctggatcagc cagttgatca
      601 ggccaattcg cgtctccacc ttcgggacga aaccgaacga cagcaagatc tcgggtcgat
      661 ggtcgcctgc cttcgcgaac tccgtcagga agcccacgat ggcgtcggag tacagcagct
      721 gggtcatgcc gtacgtggca ccttggttgc acttgaagtt gaaccggccg tgttcgccct
      781 cgcgcgtcgg aatcaggatc gcaccgcggt tgggcacgag ctggtcgtag atcgacaggg
      841 cgtcagtggg cgcgacgccg gcaccttcac cgtcgttcat ggtccgcggc acgccgacga
      901 acgcgatgcc gtcgaagccg gcgtcgcgca gatcggtcag ccgatgccgc aacgacgatt
      961 catccatgaa agccgtgacc tgggtacaca gcccgttaat cccgggcagc tccggcttga
     1021 tgatgttcca gaactccagt acgtccagcc tcggcttcat ctcgacggga cgatcgtcgt
     1081 cctcctcgat catcccgggg atcatgacgt gcccaagttg gccgtcgatg ccggtttcct
     1141 tggagaaccg cagcaccttg cgcgcttcct cgacggcctg ctcaggaccg aggtcggcgt
     1201 tcggcggcac cagctcgaac gcgacggtgt tgagggtcaa cagattgctc ctgattgcat
     1261 gcctgtctgc tccgtcccga agggcgacga tgaccgccgg ggaggatcag agcccatcta
     1321 tgcaatcgag attagcgact ggcgagtagg cgacggctgg cgcccaggga cggcgtggca
     1381 gctattgatt ggtcgtggcg attctgccat gcccggcaca gatcctgcca ccgggatgac
     1441 ttgcgtccac ctgcgcgttt tacctaacag atcggcaggc gtggtacacg gccctcctga
     1501 cgagacggcc cgaggagggg cttcacatga agaagatcac ctttgccacc gtggtgtcga
     1561 gcgcattggc ggcactcgcc attggactgg ccggtgcggc tctcgcggac gccgggagcg
     1621 gttcaaatgt gcccgcgccc gcgcccgtct acccgcagac cgccaccggt ggggcgaatc
     1681 cgtacacgcc ctacggcgtc gacccctatg tccccttcgg cgtgtggtcg cagcactgag
     1741 ccctacacgt tgccgaggat cgcggagagc gcggcctcaa cggttgcagg cggcgggaac
     1801 ggtacgacat aggcgatgac gaccgtgtct ccgtcggcgt aggcatatcc gtcggtttgt
     1861 agcggaatgt tgaagtgggt caggacatga ccgtctactt ctgcgggttc gctgggaacg
     1921 ccgacgtcct cgcgggtttg atctgacagc ttccctacgt aggtatggac gaatgtgtcg
     1981 ccgctgccgc tcttgagttg gcccacaagc actttgagaa tcggattccc gttctggctc
     2041 accacgccag cggctaggcc gccaaactcg tgagtcacaa aggcgggatc aacgtcggcg
     2101 gggcagatct cggtgacggt gaacccgggg agctcggcga acccgctggg cgctggtgtc
     2161 tgcagcccgc agtcgacatt gacgtgcgaa atcggaccgg ccgaacgggt cgtcgtcgat
     2221 ccgcacgcgg cacacgtcgc gacgaatacc gccgcgagcg ccgcggctgt gaaccgcttg
     2281 ggataaggca caattgacag catccgatgt gcgtcttgaa agattcttgg cgtcccacgg
     2341 ttggatgcct ggccacgctg acaatgctga cggcatgtga tgcgggcggt tcggacgcgc
     2401 cgccgaccag tctggcgtca cccacgtccg ccgccgccac caccacgtcg acaacggcgc
     2461 aaggcgcgcg aacgcagaag tggatcgacc tggacgtcgg cgattgcctc gccgacccgc
     2521 cgccgaccga ccccagcgtg gtcacggtgt cggtcgtcga ctgtgcgata ccgcacgcgg
     2581 ctgaggttta tctgcggacg aatgtggagg tcaatgccgc gatcgccgac gtcgcggatc
     2641 aacagtgcgg cgcgggacta cccgagtaca ccgggcaatc cggcggcccg ttcgtggtga
     2701 cgtacctgat cgactccaac caagatcgga cgtcgggcaa tccgctgccg agcactgtca
     2761 tctgcctgct gcaggcgcca ggcggtgggc cgctgacggg ctcggcgcgt cgctgacgca
     2821 gctacctcca gacagactgc agcgcaacgc cattcggttg cgacagtggc cgggggcaca
     2881 gtctaagtct gaccggaatc gtccatccgt aacctgggtg acgtgagcgt tgcatccgat
     2941 gccacggtcg tcctcgacga ccggttcgcc cgcgagctgc cggagatggc cgtgcgctgg
     3001 caggccgaga cgcctccgga cccgcagctg ctggtgctca acgagccatt ggccgcgtcc
     3061 ttgggcctgg acccggcttg gctgcgaagc cctgacggtc tgcggtttct ggtcggctct
     3121 ttggtgccca atggggccac gcccgtcgcg caggcctacg ccggaca
//
LOCUS       DASRJA010000332        11945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3815884,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000332 DASRJA010000000
VERSION     DASRJA010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11945
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3815884"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /gene="rpsA"
                     /locus_tag="VFC01_14585"
     CDS             complement(<1..1323)
                     /gene="rpsA"
                     /locus_tag="VFC01_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZN80893.1"
                     /translation="MPSPSVTSPQVAVNDIGSSEDFLAAIDKTIKYFNDGDIVEGTIV
                     KVDRDEVLLDIGYKTEGVIPSRELSIKHDVDPNEVVSVGDEVEALVLTKEDKEGRLIL
                     SKKRAQYERAWGTIEELKEKDEAVKGTVIEVVKGGLILDIGLRGFLPASLVEMRRVRD
                     LQPYIGKEIEAKIIELDKNRNNVVLSRRAWLEQTQSEVRSEFLNQLTKGAIRKGVVSS
                     IVNFGAFVDLGGVDGLVHVSELSWKHIDHPSEVVNVGDEVTVEVLDVDMDRERVSLSL
                     KATQEDPWRHFARTHAIGQIVPGKVTKLVPFGAFVRVEEGIEGLVHISELSERHVEVP
                     DQVVQVGDDAMVKVIDIDLERRRISLSLKQANEDYTEEFDPSKYGMADSYDEQGNYIF
                     PEGFDSETNEWLEGFEKQREEWESRYAEAERRHKMHTAQMEKFAAAEAE"
     gene            1499..2665
                     /locus_tag="VFC01_14590"
     CDS             1499..2665
                     /locus_tag="VFC01_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HZN80894.1"
                     /translation="MSYAGAPQPTHAYAPPFQRKIRKVGAPLAVLILLGTIAGLIVIG
                     LTAVNPVGTAIGFVLSSVTMTVVVLAYVWLDRWEPEPPRLLVLAFLWGASVAVVIASI
                     LQIYAESVINPGEMEEVSSFSVVLGAPLTEEAAKGLFLLLMMTGRRRNELNSLTDCLV
                     YAGLVGAGFAWLEDILYIANGESVSESLLTAALRLIMGPFAHSLFTTFFGIGVYFAMQ
                     QRNAAAKAGCILLGYLGAVFMHGLWNGSSLVGFEAYLLIYALWMVPVFVLVIVLGVQS
                     RRREQRVAAEKLPGMIAAGLVTPNEATWLGSLRSRKLAINEATRFGGRPAGKTVKKFA
                     AQVVELAFVRDRIDRGFGDQRVFALQQEEVNSVAAARAAAPALQSIAGYRSPGP"
     gene            complement(2662..3063)
                     /locus_tag="VFC01_14595"
     CDS             complement(2662..3063)
                     /locus_tag="VFC01_14595"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZN80895.1"
                     /translation="MADRVVDLVPPLASTSQAPETLHLNAGEVLFEQGTMGDLIYVVA
                     EGEFEIVRELSGGGEELVKVARPGDYFGEFGVLFHLPRSATVRAGTDATVIGYGVQAF
                     RDRLGVGGLGDLIAHGPLQAADRCAQERSAL"
     gene            complement(3095..3226)
                     /locus_tag="VFC01_14600"
                     /pseudo
     CDS             complement(3095..3226)
                     /locus_tag="VFC01_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214586.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease"
     gene            complement(3335..4405)
                     /locus_tag="VFC01_14605"
     CDS             complement(3335..4405)
                     /locus_tag="VFC01_14605"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN80896.1"
                     /translation="MVTLCACGPTSSAEPGPTIPATRLDAAITQTMSDMSIPGLVVGI
                     WGPAGDYVRAFGVADKTTDAPMQTDFYSRIGSVTKTFTVTALLQLVDQGKLSLDDPIS
                     KYVPGVPTGDQITLRELAQMQSGLTTYDDVAAFAESYLADPHQSFTPTQLVAFALDKP
                     LQFPPGSQFQYCNTNTVLLGLVVEKQSGQRLPDYISEHILTPLNMTHTSFPTTAAWPE
                     PHAQGYTSVTGPEQNATDWNLSWAWAAGSMISTLADMRIWAHALASGTLLRPETQRQR
                     LDTTVPMNAEKSALYGLGVFNDGGWIGHTGVIFGYQTGVFFLPQTQTTLVFYTNTDVP
                     HEVGTAIAHAITSLISPDHVYH"
     gene            complement(4503..7163)
                     /gene="polA"
                     /locus_tag="VFC01_14610"
     CDS             complement(4503..7163)
                     /gene="polA"
                     /locus_tag="VFC01_14610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZN80897.1"
                     /translation="MLLDGNSLAYRAFYALPAENFKTQGGLTTNAVYGFTAMLINLLR
                     DEQPSHIAAAFDVSRQTFRSDKYPEYKAGRSSTPDEFRGQIDITKEVLGALGITVLAE
                     PGFEADDLIATLATQAEDGGYRVLVVTGDRDSLQLVSDDVTVLYPRKGVSELTRFTPE
                     AVLEKYGLTPRQYPDFAALRGDPSDNLPGIPGVGEKTATKWIAEYGSLQALVDNVDTV
                     KGKVGDALRANLSHVVLNRELTDLVKDVPLAQTPDTLRMQPWDRDQIHRLFDDLEFRV
                     LRERLFETLASADPEVDQGFDVKGGALEAGELAAWLAEQSQGRRFGLAVVGTHLAFDA
                     DATALAIVAADGDGRYLDTATLTADDEAALSSWLADTEQPKALHEAKLAMHDLEGRGW
                     KLAGVTSDTALAAYLVRPGQRSFALDDLSLRYLKRELRADNPEQQQLSLLDDSDGVDD
                     QAVQTVILRASAVMDLADALDEELARMECSSLLGDMELPVQRALAEMETAGIAVDLKQ
                     LSELQSEFANLIRDAAEAAYAVIGKQINLGSPKQLQVVLFDELEMPKTKRTKTGYTTD
                     ADALQSLFDKTGHPFLQHLLAHRDATRLKVTVDGLLASVASDGRIHTTFNQTIAATGR
                     LSSTEPNLQNIPIRTEAGRRIRDAFVVGRGYDELMTADYSQIEMRIMAHLSRDEGLIE
                     AFRTGEDLHSFVASRAFSVPIEEVTGELRRRVKAMSYGLAYGLSAYGLAAQLKISTEE
                     AKVQMEQYFDRFGGVRDYLRDVVDQARKDGYTSTVFGRRRYLPELDSSNRNVREAAER
                     AALNAPIQGSAADIIKVAMINVDKALKDAGLKSRMLLQVHDELLFEIAPGERDALEKL
                     VKDQMCGAYPLDVPLEVSVGYGRSWDSAAH"
     gene            complement(7249..7743)
                     /locus_tag="VFC01_14615"
     CDS             complement(7249..7743)
                     /locus_tag="VFC01_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80898.1"
                     /translation="MSARIVLAAATTALSATLLTVPAPAQADPCTQYGFIGDVAIRQS
                     NGYRLEFSSTGPAATGRATATGANGATMGGNISGSIAGRNVDFTIRWDGAAGQGHYTG
                     KIGNDDFVYGGKTVDESNPASRSSWESTVPFVCLAPPAAPAPIPTPTGPPPEFTQQVP
                     RFPG"
     gene            complement(7757..9688)
                     /locus_tag="VFC01_14620"
     CDS             complement(7757..9688)
                     /locus_tag="VFC01_14620"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZN80899.1"
                     /translation="MSSASNVTRVGGLAVALGLGAAVATGHGVAAADPSDSDSSDSSA
                     SAKSTSESSDKTSESPAPPSNSSTDNAATELAPKVTEPEAAEPDNRAKPAAKKRRVSQ
                     LADASVARASGVAPRRTPTDASEVAKDQPDENATTPPTVLSTNTVSARRSVAEPDPVI
                     APAPPTSRVTQLSFASAAEANSPTSTKPVRPVGPPPLAELVFAAFRRLQSTFFNKTPT
                     ARPVQTSQAASGVLTGNVNAADADGDPLTYKVTRPAAHGTVAVSADGTYTYTPGDDFA
                     ASGATDTFVVTISEANGASHLHGPADLFNRLLRIISIGLFDPPDASTIQREITVHASV
                     VTTNIPVGKFPHDPSVRPVTGSHVYIANDDGTISVIDTATNTVVKSITINQGRLGDVA
                     VSPGGTYAYVTGGSGSNTLSVIDTATNTVTGTMQFGSIPDEIGFSPDGSFAYIFNEGP
                     TNVAVVDTETRTVVATIDIAATTGPTDLAVRPDGKRVYVANNFERTLSVIDTDPLHIG
                     YRTVIKTIPLDRPNGVAVSRDGTRVYVSHEFSGQQLGNTVSVIDSATNTVIDTITVGA
                     KPSAMAVSPDGSRLYVTNSGDDTVSVVDTATNSVVATIPVGAVPNGVAVSPDGSRIYV
                     TNRVLINGDPVFAVSVIRR"
     gene            complement(9770..10063)
                     /locus_tag="VFC01_14625"
     CDS             complement(9770..10063)
                     /locus_tag="VFC01_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80900.1"
                     /translation="MASVVSPLRCYLVEWYRAALVGETVDRFTAVLHNCAAVISAGGG
                     NVQLLTVLAVPADEVIFGVFAATSPDLVARACVQAGIPAQRLSAAIDARIPQP"
     gene            complement(10112..>11945)
                     /locus_tag="VFC01_14630"
     CDS             complement(10112..>11945)
                     /locus_tag="VFC01_14630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZN80901.1"
                     /translation="IIVIDGHRVHFAHPLLARGVYTDADPAVRRAMHGRLADIVTEPE
                     LQARHLARAAVGADQRTLKALDDAAESARIRGAPAAAAELIDLAVALGGDTSQRRIGS
                     AGHHFKAGETGRARSVLDEVIKDMEPGPLRAEALNLLAVIRLSDDSFADAAGLLEHSL
                     DEAGDEFGLRVQMLISLSFSLLNLGRLAEALGVIEGAVKDAERQQNSHLRSQALSVRV
                     ILRFMLGEGLDEADLRRALALEDRRADTPLTVRSHVHSAMLQAWTGRLEEAHREIQAI
                     RRRCIESGDESELMFVAFHSVLIEMWRGNFTDAGLVAEDTMERALQLNTEVPHCQALS
                     MRAALDAYAGRVDEARRDASEALSIAVRCGSPILMAWPLTTLGFVEVSTGDHHAALTT
                     LAPLLAIFDAMPDATEIVLAWCLPDALEALISVGRGDDADALIARIDRNGRRLDRAWM
                     LAVAARARSMLLAVQGDVAAARRAAEQAMAQQDRLPMPFERARTQLLLGQLQRRQGNP
                     AAAAETFHEALRQFEELGTPLWAERVRAELGRTNVGPRGTDPLTPAEQRVAELAASGM
                     TNRAVAAALFISPKTVEANLARVYRKLDIHSRAELGRRMGASDA"
BASE COUNT         1883 a         3833 c         3991 g         2238 t
ORIGIN      
        1 ctcggcctcg gcagcggcga acttctccat ctgcgcggtg tgcatcttgt gtcggcgctc
       61 cgcctcggcg tagcgggact cccactcctc acgctgcttc tcgaagccct cgagccattc
      121 gttggtctcg gagtcgaagc cttcggggaa gatgtagttg ccctgctcgt cgtagctgtc
      181 ggccatgccg tacttcgagg ggtcgaactc ctcggtgtag tcctcgttgg cctgcttgag
      241 gctcagcgag atgcggcggc gctccaggtc gatgtcgatg accttgacca tggcgtcgtc
      301 gccgacctga accacctggt ccgggacctc gacgtggcgc tcggacagct ccgagatgtg
      361 caccagaccc tcgatgccct cctcgacgcg gacgaacgcg ccgaacggca ccagcttggt
      421 gaccttgccc ggcacgatct ggccgatggc gtgggtgcgg gcgaagtggc gccacggatc
      481 ttcctgagtg gccttgagcg acagcgaaac ccgctcgcgg tccatgtcga cgtcgagcac
      541 ctcgacggtg acctcgtcgc cgacgttgac gacctcggac ggatggtcga tgtgcttcca
      601 ggacagctcg gagacgtgca ccaggccgtc gacgccgccg agatcgacga acgcgccgaa
      661 gttgacgatc gaggagacca cgcccttgcg gatggcgccc ttggtgagct ggttgaggaa
      721 ctcgctgcgc acctcggact gagtctgctc cagccaggcg cggcggctca gcaccacgtt
      781 gttgcggttt ttgtcgagct cgatgatctt ggcctcgatc tccttgccga tgtacggctg
      841 cagatcgcgg acgcgacgca tttcgaccag cgacgcgggc aggaagccgc gcaggccgat
      901 gtcgaggatc aggccgccct tgacgacctc gatgacggtg cccttgacgg cctcgtcctt
      961 ttccttgagc tcttcgatgg tgccccaggc ccgctcgtac tgagcgcgct tcttggacag
     1021 gatcaggcgg ccttccttgt cctccttggt gaggaccaga gcttcgacct catcgccgac
     1081 ggacacaacc tcgttggggt cgacgtcgtg cttgatggag agctcgcggg aggggatgac
     1141 gccttcggtc ttgtaaccga tgtcgagcag gacttcgtca cggtcaacct tgacgatcgt
     1201 cccttcgacg atgtcgccat cgttgaagta tttgatggtt ttgtcgatgg cggcgagaaa
     1261 gtcctcgctc gagccgatgt cgttgacggc tacttgcggc gaggtgacgg agggacttgg
     1321 catgtggtgg gttgctccgg acaggttggg tcgtagggac atgggactct tgttattggt
     1381 gtgaacccgc gtgaagcaca gacgggtacc cgtcgaggtt actcgactgt gtacatgctg
     1441 gacaaactcg gtctcctcag ctggtgaatc atgatcaccg cccggttacg ctgctctggt
     1501 gtcatacgca ggcgcgccgc agccaacgca tgcgtacgct ccgccatttc agaggaaaat
     1561 ccgcaaggtc ggcgcgccgc ttgccgtgct gattctgctc ggcaccatcg ccgggctgat
     1621 cgtgatcggg ctcaccgcgg tgaacccggt cgggaccgcg atcgggtttg tcctgtccag
     1681 cgtgacgatg acggtcgtcg tgctggccta cgtgtggctg gaccgatggg aaccggaacc
     1741 gccacggctg ctcgtcctcg cgttcctctg gggcgcatcg gtcgccgtcg tcatcgcttc
     1801 gatactccag atctacgccg aatcggtgat caaccccggc gagatggaag aggtcagctc
     1861 gttttcggtg gtgctcggcg caccgctgac cgaggaggcg gccaagggct tgttcctgct
     1921 gctcatgatg acgggtcggc gccgcaacga actcaactcg ctcaccgact gcctggtcta
     1981 cgcggggctc gtcggggcgg gtttcgcctg gctggaggac atcctctaca tcgccaacgg
     2041 agagtcggtg tccgaatcgc tactcaccgc cgcgcttcgg ctcatcatgg gcccgttcgc
     2101 gcactcgctg ttcacgacgt tcttcggcat cggcgtgtat ttcgcgatgc aacaacgaaa
     2161 tgccgccgcg aaggccggct gcatcctgct cggctacctg ggcgcggtgt tcatgcacgg
     2221 gctgtggaac ggctcgtcgc tggtgggctt cgaggcgtac ctcctcatct acgcgttgtg
     2281 gatggtgccc gtcttcgtgc tggtgatcgt gctcggcgtg cagagccgcc gcagggaaca
     2341 gcgcgtcgcc gcagagaagc tgcccggcat gatcgcggcg ggcctggtga ccccgaatga
     2401 ggcgacgtgg ctgggttcgc tccgcagccg aaagctcgcg atcaacgagg ccacccggtt
     2461 cgggggccgc cccgcaggca agacggtgaa gaagttcgcc gcccaggtgg tggaactggc
     2521 cttcgtccgc gaccgcatcg atcgcggctt cggcgatcag cgcgtcttcg ccctgcagca
     2581 ggaagaagtg aacagcgtcg cggcggcgcg ggcggcagca ccggcactgc agtcgatagc
     2641 cggataccgg tcgcccggtc cttagagtgc tgaccgttcc tgtgcgcacc gatcagccgc
     2701 ttgcaaggga ccgtgcgcga tcaggtcgcc caagcccccc acaccgaggc ggtcacggaa
     2761 cgcctggacc ccgtagccga tgacggtcgc gtcggtgccg gcgcgcacgg ttgccgatcg
     2821 cggcaggtga aacaacacgc cgaactcacc aaagtagtct cccggtcggg cgaccttcac
     2881 caattcctca ccgccaccgg acaattcacg cacaatctca aactcacctt cggccacaac
     2941 atagatcaga tcacccatcg tgccctgctc gaacagcacc tcgccggcgt tcaaatgcag
     3001 agtttcaggc gcctgacttg tcgacgcgag tggcggtacc aaatcgacga cgcgatcggc
     3061 catcggcagt atccggctgt catgggttgc gaccggcctc cacccgaaaa ccgttcgcaa
     3121 gcccggtcag aaccagcgtc atggcgaaga ccaggccggt gctggctatc gcgacgacga
     3181 agcgccgcct gcgccattgc atgtcccgca acgccgcgaa cagcatgccc gcgacgttac
     3241 cgacgcccaa ctatcgcgcc gggactttgg cgcaaacatt tgcggcggcg tcacaaaccg
     3301 caagtgccgc gatgacgagc gggccgtcga taggtcagtg ataaacgtga tcgggggaga
     3361 taaggctcgt tatcgcatgg gcaatcgccg tgcccacctc gtgtggcacg tcggtattgg
     3421 tgtaaaacac caacgtggtc tgcgtctgcg ggaggaagaa gaccccggtc tggtacccga
     3481 agatgacgcc ggtgtgcccg atccagccgc cgtcgttgaa gacgcccaga ccgtacaacg
     3541 cggacttctc ggcgttcatc ggaaccgtgg tgtcgaggcg ctgtcgctga gtctccggtc
     3601 gcagcagtgt tccgctggcg agtgcgtgtg cccagatccg catatcagcg agcgtcgaga
     3661 tcatgcttcc ggccgcccag gcccacgata ggttccagtc ggtggcgttt tgctccggtc
     3721 ccgtgacgct cgtgtatccc tgcgcgtggg gttcaggcca cgcagccgtg gtgggaaagc
     3781 tggtgtgggt catgttcaac ggcgtaagga tgtgttcgct gatgtaatcc ggcaggcgtt
     3841 gaccactctg cttctccacc actaggccta gcaggacggt gttggtgttg cagtactgga
     3901 attgactccc gggcgggaat tgcagcggtt tgtcgagcgc aaaggcgacc agttgcgtag
     3961 gcgtgaagga ctgatgcggg tcggcgaggt atgactcggc gaacgctgcc acgtcgtcgt
     4021 acgtcgtcag cccgctctgc atctgagcca gctcacgcag cgtgatctgg tcgcccgtcg
     4081 gtacgccggg gacgtatttg gagatcggat cgtcgaggct cagttttccc tgatcgacga
     4141 gctgcagcaa tgccgtgacg gtgaatgtct ttgtcacact gccgattcgg ctatagaagt
     4201 ccgtctgcat cggcgcatcg gtcgttttgt cagcgacacc gaacgcccgg acataatcgc
     4261 ccgccgggcc ccagatgccg acaacgaggc cgggaatgga catgtccgac atggtttgag
     4321 tgatcgcggc gtcgagtcga gtcgccggga tcgtcgggcc aggctctgcg ctggacgtcg
     4381 gtccgcacgc acacagcgtg accatcgcgg tcgccaatac tgcgactacc agccgaacct
     4441 gcgaagacgg tgaccggccg agcaccagcc gactctagat ctccggtcga tacggcgcac
     4501 tgctaatgcg cggcagagtc ccagctgcgg ccgtacccga cgctgacctc gagcggcacg
     4561 tccagcgggt aggcgccgca catctggtcc ttgaccagct tctccagggc gtcgcgctcc
     4621 cccggcgcga tctcgaacag cagctcgtcg tgcacctgca gcagcatgcg tgacttcagg
     4681 ccggcgtcct tgagcgcctt gtcgacgttg atcatcgcga ccttgatgat gtcggcggcg
     4741 ctgccctgga tcggcgcgtt gagcgccgcg cgctcggcgg cttcccggac gttgcggttg
     4801 ctgctgtcga gctcgggcag gtaacggcgc ctgccgaaca cggttgaggt gtagccgtct
     4861 ttgcgggcct ggtcgacgac atcgcgcagg tagtcgcgca ctccgccgaa ccggtcgaag
     4921 tactgctcca tctgcacctt ggcttcctcg gtggagatct tcagctgcgc ggccaggccg
     4981 taggcggaca gtccgtacgc cagcccgtag gacatcgctt tgacccggcg ccgcagctca
     5041 cccgtgacct cctcgatcgg cactgagaac gcgcgggatg cgacgaacga gtgcaggtcc
     5101 tccccggtgc ggaacgcctc gatgaggccc tcgtcgcggg acaggtgcgc catgatgcgc
     5161 atttcgattt ggctgtagtc ggccgtcatc agctcgtcgt aacccctgcc gacgacgaac
     5221 gcgtcgcgga tccggcgtcc cgcctcggtg cggatcggga tgttctgcag gttcggttcg
     5281 gtcgacgaca gacggccggt cgcggcgatg gtctggttga acgtggtgtg gatgcggccg
     5341 tcggaggcca ccgaggcgag cagcccgtcg acggtgacct tgagccgggt ggcgtcacgg
     5401 tgggccagta ggtgctgcag gaacggatgc ccggtcttgt cgaacagcga ctgcagcgcg
     5461 tcggcgtcgg tggtgtagcc ggtcttggtg cgcttggtct tcggcatctc cagctcgtcg
     5521 aacagcacca cctgcagctg ttttggcgaa ccgagattga tctgcttgcc gatcacggca
     5581 tacgccgcct cggcggcgtc gcggatcagg ttggcgaact cgctctgcag ctcggaaagc
     5641 tgcttgaggt cgacggcgat cccggcggtc tccatctcgg cgagcgcccg ctgcaccggc
     5701 agctccatgt cacccagcag cgacgagcac tccatcctgg ccagctcttc gtcgagcgca
     5761 tcggccagat ccatcacggc gctggcccgc aggatcacgg tctgcacggc ctgatcgtca
     5821 acgccgtcgc tgtcgtcgag cagcgaaagt tgttgctgct cagggttatc cgcacgcagt
     5881 tcgcgtttca ggtaacgcag cgacagatcg tcgagcgcga agctgcgctg tcccggccga
     5941 acgagatacg cggccagcgc ggtgtcggag gtgacgcctg ccagcttcca gccgcgacct
     6001 tcgaggtcgt gcatggcgag cttggcctcg tgcagcgcct tgggttgttc ggtatccgcc
     6061 agccaggagg acagcgcggc ctcgtcgtcg gcggtcagcg tcgcggtgtc gaggtagcgg
     6121 ccgtcgccgt cggcggcgac aattgccagc gcggtggcat cggcgtcgaa cgccagatgc
     6181 gtgccgacga ccgcgagccc gaagcgccgg ccttggctct gttcggccag ccaggcggcc
     6241 aactcgcctg cctcgagtgc cccgcccttc acgtcgaagc cctggtccac ctcggggtcg
     6301 gccgacgcga gcgtctcgaa cagacgctcc cgcagcaccc ggaactccag gtcgtcgaac
     6361 agccggtgga tctgatcccg gtcccacggc tgcatgcgca gcgtgtcggg cgtctgcgcc
     6421 agcggcacgt ccttgacgag gtcggtgagc tcgcggttga gcacgacgtg cgacaggttg
     6481 gcccgcagcg catcgccgac cttgcctttc acggtgtcga cgttgtcgac cagcgcctgc
     6541 aacgacccgt attcggcgat ccacttcgtc gcggtcttct ccccgactcc cgggatgcct
     6601 ggcaggttgt cgctcgggtc cccgcgcagc gccgcgaagt cggggtattg ccgcggggtc
     6661 aacccgtact tctccagaac tgcctcgggg gtgaaccgcg tcagttcgct cactcccttg
     6721 cgcgggtaca gcacggtcac gtcgtcgctg accagttgca gcgagtcgcg gtcgcccgtc
     6781 acgaccagca cgcggtatcc gccgtcctcg gcctgcgtgg cgagggtggc gatcaggtcg
     6841 tccgcctcga atcccggctc ggcgagcacg gtgatgccga gcgcgcccag cacctccttg
     6901 gtgatgtcga tctgtccccg gaactcatcg ggcgtggagg accggcccgc cttgtactcg
     6961 gggtacttgt cggagcggaa cgtctgccgg gatacgtcga acgccgcggc gatgtgtgac
     7021 ggctgctcat cgcgcagcag gttgatgagc atcgcggtaa agccgtagac cgcgttggtg
     7081 gtcagcccgc cctgggtctt gaagttctcc gcgggtagcg cgtagaaggc ccggtaagcc
     7141 agcgaattgc cgtccagcag catcaatgtg ggcttctcgg cggttgcgct tgcggtcacg
     7201 gccctactct atgcacggcc gccgacacgc aggagccgtg ccgcggactc agccggggaa
     7261 cctgggcacc tgctgcgtga actccggcgg aggtccggtc ggcgtcggta tcggcgccgg
     7321 agccgcgggc ggggcgagac acacgaacgg aaccgtcgac tcccaactgg acctggacgc
     7381 ggggttcgac tcgtcgacgg tcttgccgcc gtaaacgaag tcgtcgttgc cgatcttgcc
     7441 cgtgtagtgc ccctggccgg cagcgccgtc ccagcggatc gtgaagtcga cgttgcggcc
     7501 cgcgatgctg ccgctgatgt ttcctcccat cgtggcgccg tttgcgcccg tggcagtcgc
     7561 ccgtccggtt gcggctggtc cggtggagct gaactcgagc cggtagccgt tggactgccg
     7621 gatcgcgacg tccccgatga agccgtactg ggtgcaggga tccgcctggg ccggggctgg
     7681 gaccgtgagc agtgttgcgc tcaaagcggt tgttgcggcg gccaatacaa ttcttgcgga
     7741 catgagagtc ctttcctcag cgccggatca ccgacaccgc gaagacgggg tcgccgttga
     7801 tcaggacacg gttggtgacg tagatgcggc tgccgtcggg gctgaccgcc acgccgttcg
     7861 ggacggcgcc gactggaatc gtggcgacga cagagttggt ggcggtgtca accaccgaca
     7921 ccgtgtcgtc gccgctgttg gtgacataga ggcgggatcc gtccgggctg acggccatgg
     7981 cactgggttt ggctccgacg gtgattgtgt cgatcaccgt gttcgtagcg gagtcgatga
     8041 ccgacacggt gttgccgagc tgctgccccg aaaactcatg ggacacatag accctcgttc
     8101 cgtcccggct taccgccaca ccattcggcc ggtcgagcgg aatcgtcttg atgacggtcc
     8161 ggtagccgat gtggaggggg tcggtgtcga tcacagacaa cgtccgctca aaattgttgg
     8221 cgacatagac ccgtttgcca tcgggacgaa ccgcgaggtc ggtcggacca gtcgtagccg
     8281 cgatgtcgat cgtcgccacc accgttctgg tctcggtgtc gacgaccgcg acgttggtgg
     8341 gtccttcgtt gaagatgtag gcgaatgagc cgtcgggact gaatccgatt tcgtccggaa
     8401 tcgagccgaa ctgcatcgtg ccggtgacgg tgttcgtcgc ggtatcgatg accgaaaggg
     8461 tgttggagcc actgccaccg gtgacgtagg cataggtgcc gccagggctc accgcgacgt
     8521 cgccgagcct gccctgattg atcgtgatgc tcttcacgac ggtgttggtg gcggtgtcga
     8581 tcaccgagat ggtgccgtcg tcgttcgcga tgtagacatg gctgccggtc acgggccgca
     8641 ccgacggatc gtgcgggaac ttgccgacgg ggatgttggt ggtgacgacc gaagcgtgca
     8701 ccgttatctc tctttggatg gtgctggcgt cgggcgggtc gaacaggccg atgctgatga
     8761 tccgcagtag tcggttgaaa aggtctgcgg ggccgtggag atgggatgcg ccgttcgctt
     8821 cgctgatggt gacgacgaac gtgtcggttg cgccggacgc cgcgaagtca tcgccgggcg
     8881 tgtacgtgta tgtaccgtcg gcgctcaccg ccaccgtgcc gtgcgcggcg ggcctcgtca
     8941 ccttgtaggt cagcgggtca ccgtcggcgt cggcggcgtt cacgttgccg gtgaggactc
     9001 cgcttgccgc ttggctggtt tgcaccggcc gggcggtcgg cgtcttgttg aagaaggtgc
     9061 tctgcagccg gcggaacgcg gcaaacacca actccgccag gggcggtgga cccaccggac
     9121 ggaccggttt cgtggacgtg ggcgagttcg cctcggccgc cgacgcgaag ctcagctgcg
     9181 tcacccgact tgttggtggc gcaggtgcaa tgaccgggtc gggctccgca accgatcgcc
     9241 gtgcgctaac agtgttggtg ctcaacacag tcggcggggt cgtcgcattc tcgtctggct
     9301 ggtccttggc cacctccgac gcatcggttg gtgtccggcg tggcgccacc cctgaagcac
     9361 gggcgacgga tgcgtccgcc agttgcgata cgcgacgctt cttggccgcg ggcttcgctc
     9421 ggttatcggg ctccgccgct tcgggctcag tgactttggg tgccagttcc gtggcggcgt
     9481 tgtcagttga cgagttcgac ggcggtgcgg gcgattccga cgtcttgtcc gatgactcgc
     9541 tcgtcgactt ggccgacgcg gaggaatccg atgaatccga atcggacgga tccgccgccg
     9601 ccacgccgtg tccggtggcg accgccgcac ccaatcccag ggccaccgcc aacccgccaa
     9661 cccgcgtcac attgcttgct gagctcatca tccctctccc ggcggtcgca tcgatcacgt
     9721 gcggattgac cgtaggagct tgggaattgg agattcatcg ggtgtttccc tatggctgcg
     9781 gtatgcgggc gtcaatggcg gcactcagcc gttgggccgg gatgcccgcc tgcacacaag
     9841 ctcgggcaac gagatccggt gacgtcgcgg cgaacacacc gaagatcacc tcgtcggcgg
     9901 gtacggcgag caccgtcagc agttgaacat ttcccccacc ggcggatatc accgcggcgc
     9961 agttgtggag gacggccgtg aagcggtcca cggtctcgcc gacgagggca gcgcgatacc
    10021 actcgacgag gtagcaccgc aaaggcgaca caacgctggc caccgatccg acgctagcga
    10081 tgtcaaaccc cggatttcgt cgggtgtttc cctatgcgtc ggatgcgccc atgcgcctgc
    10141 cgagctcggc acgcgagtgg atatcgagtt tgcgatagac gcgcgcgaga ttggcctcaa
    10201 cggttttcgg gctaatgaac agcgcagcgg cgacggcccg attggtcatg cccgacgcgg
    10261 cgagctcggc gacgcgctgt tcagcaggcg tgagcgggtc ggttccgcgt ggtccgacgt
    10321 tggtgcggcc gagttcagcg cgcacccgtt cggcccacaa cggagttccc agctcttcga
    10381 actggcgcaa cgcttcgtga aacgtttcgg ctgcggcggc tgggttgccc tgcctgcgct
    10441 gtagctgtcc caacagcagt tgagtacgtg cgcgttcgaa cggcatcggc agccggtcct
    10501 gctgagccat cgcctgctcg gcggcccggc gggcggccgc gacatcgccc tgcacggcca
    10561 gcaacatgct gcgtgcgcgg gccgcgacgg ccagcatcca tgcgcggtcc agcctgcggc
    10621 cgttgcggtc gatcctcgct atcagcgcat cggcgtcgtc gccacgcccg accgagatca
    10681 aggcttccag cgcatcgggc aggcaccacg caagcacaat ctccgtcgca tcgggcatcg
    10741 cgtcgaagat tgccagcagt ggcgccagcg tcgtcagcgc cgcgtggtgg tcccccgtcg
    10801 acacctcgac aaaacctagt gtggtcagcg gccacgccat caggatcggc gagccgcacc
    10861 gcaccgcgat cgagagtgct tcggaggcgt cgcgtcgcgc ctcgtcgacg cggcccgcgt
    10921 aggcgtcgag tgccgctcgc atcgacagcg cctggcaatg cgggacctcg gtgttgagct
    10981 gcagcgcccg ctccatcgtg tcctcggcca ccagtccggc gtcggtgaag tttccccgcc
    11041 acatctcgat cagaacgcta tggaacgcga cgaacatcag ttcgctttcg tcgccggatt
    11101 cgatgcaccg ccggcggatc gcctgtattt cccggtgggc ctcctcgagc cgaccggtcc
    11161 acgcctgcag catcgcgctg tgcacgtgcg aacggacggt caacggggtg tcggcccgcc
    11221 ggtcctccaa cgccagggcg cgtcgcaggt ctgcctcgtc gagtccctcg ccgagcatga
    11281 atcgcaggat cacccgcaca ctgagcgcct ggctgcgcag gtgtgaattc tgctgccgct
    11341 cagcgtcttt gaccgcgccc tcgatgacgc caagcgcttc ggccagccga ccgaggttca
    11401 agagcgagaa cgacagcgat atcagcatct gtacgcgcaa cccgaattcg tcacctgcct
    11461 cgtcgagaga gtgttccagc aggccggcgg catcggcgaa actgtcgtcg gaaagccgga
    11521 ttacagcgag taggttcagc gcttcggccc gcagtggccc gggttccatg tccttgatga
    11581 cctcgtcgag caccgatcgt gcccgcccgg tttcgccggc cttgaaatgg tggccggccg
    11641 agccgatccg acgctgcgag gtgtccccgc cgagggccac tgcgagatcg atcaattcgg
    11701 ccgcggcggc aggtgcgccc cggattcgtg ccgactctgc ggcgtcgtcg agtgccttca
    11761 gggtgcgctg gtccgctccg actgcggcac gtgcgaggtg cctggcctgc agctcgggtt
    11821 cggtgacgat gtcggcgagc cgcccgtgca tcgcccggcg caccgctgga tccgcgtccg
    11881 tgtagacgcc gcgggccagc agcggatgtg cgaaatgcac cctgtgcccg tcgatcacga
    11941 tgatt
//
LOCUS       DASRJA010000333         5244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1747877,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000333 DASRJA010000000
VERSION     DASRJA010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747877"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1011
                     /locus_tag="VFC01_14635"
     CDS             34..1011
                     /locus_tag="VFC01_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZN80902.1"
                     /translation="MARISFSCILAVLVIAVAACAGAERSAPTTTSSAPNGGAQRATV
                     ALITHQAPGDTFWDLVRRGAEAAAAQDNVELQYSHDPDAGAQANLVRSAVSAKVAGIA
                     VTLAAPAVMANAVQNAIAAGIPVVAVNSGIADWKPLGVMEYFGQDETIAGQAAGERFT
                     RAGAQNALCVIHEPGNVALEARCDGLAKGFTGKTQKLYVDGVDKAVVNPAVMAKLQQD
                     FSIDRVLTLGAPIALAAREAVVEVNSYAKVATFDTNAGVVDAIKNGMIEWAIDQQPFL
                     QGYLAVNSLWLYFTNKNVIGGGQPTLTGPSFVDETNINSVADLAEAGTR"
     gene            1018..2088
                     /locus_tag="VFC01_14640"
     CDS             1018..2088
                     /locus_tag="VFC01_14640"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HZN80903.1"
                     /translation="MTVLFLTGDVMTGRAIDQLLPHPGDPTLRESVVSDARTYIRLAE
                     QTNGPIPAPIDFAWPWGEALEIVDQFAPDVRLLNLETSITAGGEFARGKGVHYRMHPD
                     NIGCVAAIRPDVCALANNHILDFGYLGLSETLRTLENAGIHSAGAGLDAKSAERPAVV
                     GHVVVASCGMGSSGIPHDWAATGRRPGVAYVADMSDRSADAIADRVLPLKGSGDIAVV
                     SLHWGSNWGYNIDAAQIRFAHRLIDAGIDLVHGHSSHHPRPIEVYRGKLIMYGCGDTI
                     DDYEGISTYESYRHELRLLYFASVDECILRMVPMRMRKMRLERAPEEDADWLRASVAE
                     MSGSFGTRVETAADGVLIVRAA"
     gene            complement(2195..3463)
                     /locus_tag="VFC01_14645"
     CDS             complement(2195..3463)
                     /locus_tag="VFC01_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN80904.1"
                     /translation="MSDIEAQDFFRDDELVKDPYPFLADLRSKCPVHREPHHDVVMVT
                     GYDEALAVLGDAATFSSCMSVTGPFPGFPVPLEGEDVTELIAAHRDAIPMSDQIITLD
                     PPTHTDRRALLMGLITPKRLKENEAFMWELADRVLDSFLTGGGGEFIGEFAGPFSLLV
                     IADLLGVPEEDRDEFAAVLGRRSEAGVGSTADDTLPHGPLEYLYERFSGYIEDRRREP
                     RDDVLTGLATATFPDGSTPEVIDVVRVATNVFSAGQETTVRLLSSALKVLAERPDIQQ
                     LLRRERDRIPNFIEETLRIESPVKGDFRLTRVPSTVGGVDIPAGTILMVLNGAANRDP
                     KQFDDPDTLDPLRPNARRHIAFGRGVHSCPGAPLARAEARVCLERLLDRTTDIRIAEH
                     SHGPAENRHYEYVPTFILRGLTELHLEFTL"
     gene            3576..4151
                     /locus_tag="VFC01_14650"
     CDS             3576..4151
                     /locus_tag="VFC01_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN80905.1"
                     /translation="MANQVIETTDTRTRLIEVAIDLFTRHSFAGTSLQMIADELGFTK
                     AAIYHHFRTREQLLAAVLEPILDELRTVITSAETRRTPHARAEHMLSGYASLAVRNRG
                     LVAVLAADPSVATVLTERAEWGDLIKRQLALLADVDPGPAGAVKAAMVFAGMAGAAGP
                     TGANLDDDVLREHLVDAGRRTLGLRAPRRAM"
     gene            4197..4898
                     /locus_tag="VFC01_14655"
     CDS             4197..4898
                     /locus_tag="VFC01_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80906.1"
                     /translation="MKTAVVTGGGSGIGRAVADRLRADGYHVATIDLNRSDDEFAQTA
                     DVTDRAQVDAALAAIRAQLGPVTILVNAAGLDGFKRFTDITFDEWQKVVDVNLNGVFH
                     CIQAVLPDMVEAGWGRIVNISSSSTHSGTPYMSHYVAAKSAVNGLTKSLALEYGPAGI
                     TVNAVPPGFIDTPMLRNAESRGRLGDIQATIDATPVRRMGKPEDIAAACAFLISDEAG
                     YITGQILGVNGGRNT"
     gene            4928..>5244
                     /locus_tag="VFC01_14660"
     CDS             4928..>5244
                     /locus_tag="VFC01_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HZN80907.1"
                     /translation="MSTEPAHFTLTDGGAFMPSPFAQSHWGDDHLNGPAIVGLAARAL
                     ENHCGSPDFMPTRFTADLFRAARNALTTIDVRIVRDGRRVRSAECDVVQDGRTVARAT
                     LVQ"
BASE COUNT          970 a         1616 c         1746 g          912 t
ORIGIN      
        1 ggtcgcgctg gctcgcgtgg gaggggcgga tcgatggccc gaatatcttt ttcctgcatc
       61 ctcgcggtgc tggtgatcgc ggtcgcggcg tgtgcggggg cggagcgcag tgcaccgacg
      121 acgacgtcga gcgcccccaa cggcggcgca cagcgcgcga cagtcgcgct catcacccac
      181 caggcaccgg gcgacacctt ctgggatctg gttcgacggg gcgccgaagc cgctgctgcc
      241 caagacaacg tcgagctgca gtactctcac gacccggacg cgggggccca ggccaacctt
      301 gtgcggtcgg cggtgagcgc gaaggtcgcc ggcatcgccg tgacgcttgc cgctcccgcg
      361 gtcatggcca atgcggtgca gaatgcaatc gccgccggaa ttccagtcgt ggcagtgaat
      421 tccggcatcg ccgactggaa accgctgggg gtgatggaat acttcggaca agacgaaaca
      481 atcgcgggcc aggccgccgg cgaacgcttc acccgcgcgg gcgcgcagaa cgcgctgtgt
      541 gtgattcacg agccgggcaa tgtcgcactc gaggcgcgat gtgacgggct ggcaaaggga
      601 ttcaccggca agacgcagaa gctgtacgta gacggcgtgg acaaggcggt cgtcaaccct
      661 gcagtgatgg caaagctgca acaggacttc agcatcgacc gtgtcctcac ccttggtgcc
      721 ccaatagcat tggccgcgcg ggaagcggtc gtggaagtga acagctacgc caaggtggcg
      781 acgttcgaca caaacgcggg cgtggtcgac gcgatcaaga acggcatgat cgagtgggcc
      841 atcgatcagc agccgttcct ccaggggtat ctggcggtca actcgctgtg gctgtacttc
      901 accaacaaga acgtgatcgg cggtggccag ccgaccttga ccggaccgtc tttcgtcgat
      961 gagaccaaca tcaactctgt ggccgacctc gccgaggccg gcacccggtg agccgccgtg
     1021 acggtcctct tcctcaccgg cgacgtgatg accggccgtg cgatcgatca actactaccg
     1081 cacccgggtg atcccacgct gcgcgaatcc gttgtgtccg atgcacgtac ctacatccgg
     1141 ctagcagagc agacgaacgg cccgattccg gcgccgatcg acttcgcctg gccatggggc
     1201 gaagcgctgg agatcgtgga ccagttcgcg ccggatgtcc ggttgctcaa tctggaaacc
     1261 agcatcaccg ccggcggtga gttcgcgcgg gggaaagggg tgcactaccg gatgcatccg
     1321 gacaacatcg gatgtgtggc cgcgattcgg ccagacgtgt gcgcgctggc caataatcac
     1381 atcctcgact tcggttatct gggactatcc gagacgcttc ggacactcga gaacgccggg
     1441 atacacagcg ccggtgcggg attggatgcg aagagtgccg agcggcccgc agtcgtcggg
     1501 catgtcgtgg tcgcatcatg tggcatggga tcgagcggta ttccgcacga ctgggcggca
     1561 acaggacgtc gtccgggtgt cgcatacgtt gccgatatgt ccgaccgtag cgccgacgcg
     1621 atcgccgatc gggtgttgcc gttgaagggc tctggcgata tcgcggtggt gtctttgcat
     1681 tgggggtcga attggggcta taacatcgat gccgcgcaga tacgcttcgc gcacaggttg
     1741 attgacgcgg gcatcgatct ggtgcacggt cattcgtcgc accatccccg gcccatcgag
     1801 gtgtaccgcg gcaagctgat catgtatggg tgtggcgaca ccatcgacga ctacgaaggc
     1861 atctcgacct acgagtcgta ccgccatgaa ctccgattgc tgtacttcgc ctcggtcgac
     1921 gaatgcattc tgcgcatggt gccgatgcga atgcgaaaga tgcggctcga gcgcgcgccc
     1981 gaagaggacg cagactggtt gcgcgcgagc gtggcagaga tgagcggcag cttcggaacc
     2041 cgcgtcgaga ccgcggccga cggagtgctg atcgtccgcg cagcttagtg ctgtgcagct
     2101 tcgccgaaac tgaattccag caggccctta ctcgcactat ccctgcatgg atgcaggctc
     2161 gctgaagtgc ggtagtgcgt cttctccgcg ccgcttagag cgtgaactcg agatgtagtt
     2221 cggtcaaccc gcgcaggatg aaggtcggca catactcgta gtgccgattc tcggccggac
     2281 catgggagtg ttcggcgatc ctgatgtccg ttgtgcggtc aagaaggcgt tcgaggcaga
     2341 cccgtgcctc ggcgcgcgcc agcggagcac cggggcaaga atgcacgcct cggccaaaag
     2401 cgatgtgacg ccgggcgttt ggacgcaacg gatcgagggt gtcggggtcg tcgaactgct
     2461 tcgggtcgcg attggccgca ccgttgagca ccatcaggat ggtgccggcc ggaatgtcga
     2521 cgccgccaac ggtgctcggc acccgcgtca gccggaagtc acccttgacc ggactctcga
     2581 tgcgcagggt ctcctcgatg aagttgggga tgcggtcgcg ctcacgccgc agcagctgct
     2641 gtatgtcggg tcgctcggcg aggaccttca gagccgagct cagcaggcgc acagtggttt
     2701 cctggccggc tgagaacacg ttggtggcaa cgcgcaccac gtcgatgacc tcgggggtcg
     2761 agccgtccgg aaacgtcgca gtggccaaac cggtgaggac atcgtcgcgc ggctcgcggc
     2821 gacggtcctc gatgtagccc gagaaccttt cgtagagata ttccagcggg ccgtgcggca
     2881 gggtgtcgtc ggcggtgctg ccgacgccgg cttccgatcg tcttccgagc accgcggcga
     2941 actcgtcacg gtcctcctcg ggcacaccca gcagatcggc gatcaccagc aggctgaacg
     3001 gtccggcgaa ttcgccgatg aactcaccgc cgccgccggt gaggaaagaa tccaggacgc
     3061 ggtcagccaa ctcccacatg aacgcctcgt tctccttgag acgcttcggc gtgatcagcc
     3121 ccatcagcag ggcccggcgg tcggtgtggg tgggcgggtc gagcgtgatg atctggtcgc
     3181 tcatcggaat ggcatcgcga tgcgctgcga tcaactcggt gacatcctcg ccctcgagcg
     3241 gcacggggaa cccggggaag ggcccagtca ccgacatgca cgacgagaac gtcgcggcgt
     3301 cgccgaggac cgccagcgcc tcgtcgtagc ccgtcaccat gacgacgtcg tgatgcggtt
     3361 cgcggtgcac cggacacttg ctgcgcaggt ccgccaggaa ggggtacggg tccttgacca
     3421 gctcgtcgtc gcggaagaag tcctgcgctt cgatgtcgct catcggcaaa tgctcctctg
     3481 tgacctagtc gatcgtaaag gaactagctt tacgatcggc tagtacagta ccatacgatc
     3541 gtaaagcttc ggcgggacga tttgaggagc tgacgatggc gaaccaggtc atcgagacca
     3601 ccgatacccg gacgcgtctg atcgaggtcg ccatcgacct gttcacccgg cacagcttcg
     3661 cgggcacgtc gttgcagatg atcgccgacg agctggggtt cacgaaagcc gccatctacc
     3721 accatttccg gacccgtgag caactgctgg ccgcggtcct cgagccgatt ctcgacgagc
     3781 tacgaaccgt catcacatcc gcggagacga ggcgcacacc ccacgcgcgg gcggagcaca
     3841 tgctcagcgg ctacgcctca ctggcggtgc gaaaccgggg acttgtcgct gtgcttgccg
     3901 ccgatcccag cgtggcgact gtgctgaccg aacgcgccga atggggcgat ctgatcaagc
     3961 ggcagcttgc actgctggcc gatgtcgatc cgggaccggc tggtgcagtc aaggcggcca
     4021 tggtgttcgc cgggatggcc ggtgcggcgg gtccgacagg ggccaacctc gacgacgacg
     4081 tgctgcgtga acatctcgtg gacgccggcc gtcgcacgct aggcctgcgc gcgcctcgac
     4141 gcgcgatgta atttccaatt acccatgtag tcaacagatc tcggaaggaa cctattgtga
     4201 agacggctgt ggtcactgga ggcggctcag gaatcggtcg cgcggtggcg gatcggctgc
     4261 gggccgatgg ctatcacgtg gcgaccatcg acctgaaccg atccgatgac gaatttgcgc
     4321 agaccgcgga tgtgaccgat cgagcgcagg tagatgccgc gctggcggcg atccgcgcac
     4381 aactcgggcc ggtgacgatc ctggtcaacg cggccggtct cgacgggttc aagcggttca
     4441 ccgatatcac gttcgacgag tggcagaaag tggtcgacgt caatctcaac ggcgtgttcc
     4501 actgcattca ggcggtgctg cccgacatgg tcgaagcggg ctggggacga atcgtgaaca
     4561 tctcgtcatc cagcacccat tccggcacgc cctacatgtc gcactatgtg gccgcgaagt
     4621 cggcagtcaa cggcctgacc aagtcgctgg cgctggagta cggccccgcg ggcatcaccg
     4681 tgaacgcggt gccgccgggc ttcatcgaca ccccgatgct gcgcaacgca gaatcgcgcg
     4741 gcaggctcgg cgacattcag gcgaccatcg acgcgacccc ggtccgtcgg atgggtaagc
     4801 ccgaagacat cgctgccgca tgcgctttcc tgatctccga tgaggccggc tacatcacag
     4861 ggcagatcct gggcgtcaac ggcgggcgta acacctagcc gatcaggcgc tatgcgacga
     4921 tggtcggatg tcgacggagc cggcgcattt cacactgacc gacggtggtg cgttcatgcc
     4981 gtcgccgttc gcgcaaagtc attggggcga cgatcatctc aacggacccg ccattgtggg
     5041 tttggctgcg cgtgcgctgg aaaaccattg cggatcaccg gatttcatgc cgactcggtt
     5101 caccgccgac ttgttccgag cggcacgcaa cgcgcttacg acaatcgatg tccgaattgt
     5161 gcgcgacgga cgacgcgtgc gcagcgcgga atgcgatgtg gtacaggacg gccgcacggt
     5221 agcgcgggcc acgctggtgc aata
//
LOCUS       DASRJA010000334         7264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2169997,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000334 DASRJA010000000
VERSION     DASRJA010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2169997"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1198
                     /locus_tag="VFC01_14665"
     CDS             44..1198
                     /locus_tag="VFC01_14665"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZN80908.1"
                     /translation="MSVALRAGIPPFYVMDVWLAAAERQRTYGDLVNLSAGQPSAGAP
                     TAVREAAKAALDSGPLGYSVALGIPELRTAIASSYADRQGLAVDSDDVVITTGSSGGF
                     LLAFLACFDVGDRVAIASPGYPCYRNILSALGCEVVEMPCGPETRFQPTAQMLAELDP
                     PVQGVIVASPANPTGTVIPPEELTAIAAWCDQSGARLISDEVYHGLVYEGAPATSCAW
                     QTSRNAVVVNSFSKYFAMTGWRLGWLLVPRELLRAVDCLTGNFTICPPVLPQYAAVAA
                     FTPESVAEADALLHHYAANRQMLLDGLRSIGIDRLAPTDGAFYVYADVSHLTTDSLSF
                     CSKLLNDTGVAIAPGIDFDTVRGGSFVRLSFAGPTGDIEEALRRIGSWLG"
     gene            complement(1195..3102)
                     /locus_tag="VFC01_14670"
     CDS             complement(1195..3102)
                     /locus_tag="VFC01_14670"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HZN80909.1"
                     /translation="MTHAIDVPQWQPTERDIAEARVTDFARFVEQRTAASFTDYQSLW
                     QWSVDDPAAFWGALWEYFGFGDAPSTVLENAQMPGARWFPGVQLNYVDQIVRNARTDR
                     PAILHVSEDGRITELSWHELLGRTAAFVQTLRSHGVTAGDRVVGYLPNIPEAVIAFLA
                     TASIGAVWSACGQDYSAKAALDRLGQLEPVVLVTADGYQYGGKSHDKSDDVAALQAGL
                     PTLRATVMASELAAEADPSELETVPVDFDHPLWILFSSGTTGLPKGIMHGHGGVVLEH
                     LKALALQSDIGTDDTFFWYTSPSWMMWNFQVSGLLAGATIVCYAGSPSYPRPDALWDI
                     ASRVRATILGTSPAYVLGCGKADAVPRTEHDLSALRTVGITGSSLPPSSSLWLRDNVG
                     EHVQVASISGGTDVVSAFIGGVRTVPVWPGELSAPYLGVAMDAWDESGKPVRGEVGEL
                     VITKPMPSMPIAFWNDADGSRYRSAYFEMFPGVWRHGDWITITDHGSIIVHGRSDSTL
                     NRHGIRMGSADIYQAVERIPEIAEALVIGAEQPDGGYWMPLFVVLADGAELTDGLRDR
                     IKRTIRDEVSPRHVPDEVIVAPGVPHTRTGKKLEVPIKKLFQGADPARVVEKSAVDDP
                     ALLDWYANLKR"
     gene            complement(3099..3845)
                     /locus_tag="VFC01_14675"
     CDS             complement(3099..3845)
                     /locus_tag="VFC01_14675"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HZN80910.1"
                     /translation="MSDLSGRTALVTGGASGIGAACARELAARGATVTVADVDESGAK
                     DLAADIGGHAWAVDLLDVSSLENLQLETDILVNNAGVQSINAIADFPPDKFRMMLALM
                     VETPFLLIRAALPHMYRQKFGRVINISSVHGIRASEYKVAYVTAKHGLEGLSKVTALE
                     GGPHGVTSNCVNPGYVRTPLVTKQIADQARTHDIPEDQVIAEILLKESAIKRLVEPEE
                     VAALVGWLASPAAGMVTGASYMMDGGWSAR"
     gene            complement(3842..5218)
                     /locus_tag="VFC01_14680"
     CDS             complement(3842..5218)
                     /locus_tag="VFC01_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN80911.1"
                     /translation="MSVRDETRPVSDEPGPIATGLKRVVAASMAGTVVEWYEFFLYGT
                     AATLVFSKVFFAKGDSDLDAILAAFLTYAVGFAARPIGGVVFGHFGDKYGRKKLLQFS
                     LLLVGAVTFLMGCLPTFGQVGYWAPALLVVLRFLQGFAVGGEWGGAVLLVAEHSPNQS
                     RAFWASWPQAAVPVGNMLATVVLLVLTTTLSDDAFLSWGWRVAFWLSAVVVLIGYYIR
                     TKVTDAPIFVAAQQEAERVKAVSYGVVEVLKRYPRGVFTAMGLRFAENIMYYLVVTFS
                     ITYLKVQVGADTSDILWWLLAAHAVHFVVIPLVGRLSDRVGRRPVYFVGALAAGTWGF
                     FAFPMMNSANYLVIMGAIIIGLVIHAIMYAPQPAIMAEMFPTRMRYSGVSLGYQVTSI
                     VAGSLAPIIAVKLLEIFDSSVPIALYLAGAAVITLIAVYFARETKGIDLASVDQADRD
                     DLAKTGVA"
     gene            5332..6252
                     /locus_tag="VFC01_14685"
     CDS             5332..6252
                     /locus_tag="VFC01_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN80912.1"
                     /translation="MTGPSGGRRPSADDLLVLLAVGRSGRYNTAAEELGLNHTTISRR
                     IAALEQSIGGRVLARVGGGWELTDLGREALAAAEAVDSAVRSLTVDPGGVRTLEGVVR
                     ISATDGFSAYIAAPAAAQVQRRHPKVAVEIVAATRRATQQRSGLDVEVVVGEPKVHRA
                     KAIRLGDYCLGLYGSRDYLSEHGTPTSIADLARYPLVYFIDSMLQVDDLDIATSFAPA
                     MRESVTSTNVFVHVEATRAHAGIGMLPCFMADRHDDLIRVLPDAVAIQLTYWLVTRAE
                     TLRRPEVAAVVDAIRHRVHDQRDVLLGRHQ"
     gene            complement(6246..6500)
                     /locus_tag="VFC01_14690"
     CDS             complement(6246..6500)
                     /locus_tag="VFC01_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80913.1"
                     /translation="MFESYELPDPEDIVALDDAGLINTVVMTTVLEDLVTACRALALD
                     ELCIRRTFPSASTCPAPTRPRAITSSRRRQRKARKRRRRH"
     gene            6584..>7264
                     /locus_tag="VFC01_14695"
     CDS             6584..>7264
                     /locus_tag="VFC01_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN80914.1"
                     /translation="MPFIDHPKGRAYFRHWAAVEPRAAVIFLHGFGEHTGVYHRYGFA
                     LNAAGIDLWAVDQFGHGLSPGDRGDFGSIEDSSHLADSLTELAEGARSGLPLIAQGHS
                     FGSVVTLFRLLEQPERYRAGIISGAPLVPIPEMLDADSSFDLDPGWLSGDPFYIDSLE
                     NDPLAFVDADGKPLARELDKAWDRFGSELPKLAVPTLAVHGTNDPIAPVDAVRAYADQ
                     LDALQLKEF"
BASE COUNT         1274 a         2410 c         2350 g         1230 t
ORIGIN      
        1 gctaccgttt cgagcgagca gcccgcttgt tagcctcgct ccaatgagcg ttgcgctgag
       61 ggccgggatt ccaccgttct acgtgatgga cgtgtggctg gccgccgcgg agcgccagcg
      121 cacgtacggc gacctggtca acctgtccgc cggccagccg agcgctggcg cacccacggc
      181 ggtgcgggag gcagccaagg cagccttgga cagcggccca ctcggctact cggtcgcact
      241 gggcatcccc gagctgcgca ccgcgatcgc aagctcgtac gcggaccgcc aaggcctcgc
      301 cgtcgactca gacgacgtcg tgatcaccac cggctccagc ggtggattcc tgctcgcctt
      361 cctggcgtgc tttgacgtcg gcgaccgcgt ggccatcgcc agccctggct acccttgcta
      421 ccgcaacatc ctgtcggcgc tgggctgtga ggtcgtcgaa atgccgtgtg ggccggagac
      481 ccgattccag ccgacggccc agatgctggc cgagctggac ccgccggtgc agggcgtgat
      541 cgtggcgagc ccggccaatc ccaccggaac ggtgattccg cccgaggagc tgaccgcgat
      601 cgcggcctgg tgcgatcaat cgggtgcgcg cctgatcagt gacgaggttt atcacgggct
      661 ggtgtacgag ggcgcgccgg cgaccagctg tgcgtggcag acctcacgaa atgccgtggt
      721 ggtcaacagt ttttcgaagt acttcgcgat gacgggctgg cggttgggct ggctgctggt
      781 gccgcgtgaa ctgcttcgcg cagtcgactg cctgaccgga aacttcacta tctgcccgcc
      841 ggtgctgccg cagtacgccg ccgtcgcagc cttcaccccg gaatcggtcg ccgaggcgga
      901 cgcactgcta catcactacg cggccaacag gcagatgctg ctcgacggcc tacgctcgat
      961 cggcatcgac cggctcgccc ccactgacgg cgcgttctac gtctacgccg atgtctcgca
     1021 tctgactacg gactcgttgt cgttctgctc gaagctgttg aacgacaccg gcgttgcgat
     1081 cgcgccgggc atcgatttcg acacggtgcg cggcggctcg ttcgtgcggt tgtcgttcgc
     1141 ggggccgacg ggcgacatcg aggaagcgtt gcgccgcatc ggatcctggc tgggctagcg
     1201 cttcaggttg gcgtaccaat cgagcagcgc cggatcgtct acggcactct tctcgacgac
     1261 gcgcgcgggg tcggcgcctt ggaacagctt cttgatcggc acctcgagct tcttacccgt
     1321 gcgggtatgc ggaacaccgg gggcgacaat gacctcgtcg ggcacatgcc gcggcgatac
     1381 ctcgtcgcga atggtccgct tgatgcggtc gcgtagcccg tcggtcaatt cggcgccgtc
     1441 agcgagcacg acgaacaacg gcatccaata gccgccgtcg ggctgttcgg ccccgatcac
     1501 caacgcctcg gcgatctcgg ggatccgctc gacggcctgg tagatgtcgg cgctgcccat
     1561 ccggatgcca tgacggttca gcgtcgagtc ggatcgtcca tgcacgatga tgctgccgtg
     1621 atcggtgatc gtgatccagt cgccgtgccg ccaaacaccg gggaacatct cgaaatatgc
     1681 actgcgataa cgtgatccgt cggcatcatt ccagaacgcg atgggcatgg acggcatcgg
     1741 cttggtgatg accagctcgc cgacctcacc gcgtaccggc ttgcccgatt cgtcccacgc
     1801 gtccatcgcg acacccaggt acggcgcgga caactccccc ggccacaccg gcacggtgcg
     1861 cacaccgccg atgaatgccg acaccacatc ggtgccgccg ctgatggagg ccacctggac
     1921 gtgttcaccg acgttgtcgc gcaaccacag cgacgacgat ggtggcagcg atgagccagt
     1981 gatgccgacg gtccgcagcg cggataggtc gtgctcggtg cgcggcaccg cgtccgcttt
     2041 gccacaaccc agcacgtacg ccgggctggt gcccagaatc gtcgctcgga cgcgggaggc
     2101 gatgtcccat aatgcgtcgg gtcgcggata cgacggactg ccggcatagc agacgatcgt
     2161 cgcgcctgct agaaggcccg agacctggaa gttccacatc atccagctcg ggctggtgta
     2221 ccagaagaat gtgtcgtcgg tgccgatatc ggattgcagc gcaagggctt tgaggtgttc
     2281 gagcacgacg ccgccgtgcc cgtgcatgat tcctttcggc agcccggtgg tacctgacga
     2341 aaatagaatc cacagcggat ggtcgaaatc aactggcacc gtctccagtt cgcttgggtc
     2401 ggcctcggcg gcgagttcgg atgccatcac ggtggcccgc agcgtcggca ggccggcctg
     2461 tagcgcggcg acgtcgtcgc tcttgtcgtg ggacttgccg ccgtactggt agccgtcggc
     2521 ggtgaccagg acgaccggct ccagctggcc gagccggtcc agcgccgcct tggcggaata
     2581 gtcctgtccg caggcgctcc aaacggcgcc gatgctcgcg gtggcgagaa acgcgatgac
     2641 cgcctcgggg atgttgggca gatacccgac gacgcgatca cccgcggtga ccccatgcga
     2701 acgcagcgtc tgcacgaatg ccgcagtgcg cccgagcaat tcgtgccatg acagttcggt
     2761 gatccgtccg tcctcgctga cgtgcaggat ggcgggccgg tcggtacgcg cgttgcgcac
     2821 gatctggtcg acgtagttca gctgcacacc ggggaaccag cgtgcacccg gcatctgcgc
     2881 gttctcgagc acggtcgacg gggcgtcgcc gaaaccgaag tactcccaca gcgcgcccca
     2941 gaacgccgcc gggtcgtcga ccgaccactg ccacaaagac tggtagtcgg taaacgatgc
     3001 tgctgtgcgc tgctcgacga accgcgcgaa gtcggtgacg cgcgcctccg cgatgtcgcg
     3061 ctccgtcggc tgccactgcg gaacgtcgat ggcgtgcgtc atcgcgcgct ccagccgccg
     3121 tccatcatgt acgacgcccc ggtcaccatt cccgcggccg gtgaggccag ccaaccgacc
     3181 agtgcggcaa cctcttccgg ctcaaccagt ctcttgattg cgctctcctt caacaggatc
     3241 tcggcgatca cctgatcttc tgggatgtcg tgcgtacggg cctggtcggc aatctgcttg
     3301 gtgaccaaag gagtacggac gtacccgggg ttcacgcagt tgctcgtcac accgtgtgga
     3361 ccgccctcaa gcgccgtcac cttcgacaga ccctcgagcc cgtgtttggc ggtgacgtag
     3421 gcgaccttgt actccgaagc gcgaatgccg tgcaccgatg agatgttaat gacgcggccg
     3481 aacttctgcc ggtacatgtg tggaagtgcc gcccggatca acagaaacgg cgtctcgacc
     3541 atcaatgcga gcatcatgcg gaacttgtca ggtgggaagt cggcgatcgc gttgatgctc
     3601 tgcacgccgg cgttgttgac cagaatgtcg gtctcgagct gcaggttttc cagcgatgag
     3661 acgtcgagga ggtctaccgc ccaggcgtgc ccgccgatat cggccgccag atccttcgcg
     3721 ccggactcgt cgacatcggc gaccgtgacc gtcgcgccgc gggcggccag ttccctggca
     3781 cacgccgcac cgatcccgct cgcccctccc gtgacgagcg cggtacgacc gctcagatcg
     3841 ctcatgcaac gcccgtcttg gcgaggtcgt cgcggtcggc ctggtcgacg ctggccaggt
     3901 cgatgccctt ggtctcgcgc gcgaagtaca ccgcgatgag tgtaatgacg gcggcaccgg
     3961 ccaggtaaag cgcgatcgga acagaggagt cgaagatctc gagcagtttg accgcgatga
     4021 tcggtgccag cgaaccggcg acgatcgagg tgacctgata gccaagggac acaccggaat
     4081 accgcatgcg cgtcgggaac atctcggcca tgatcgccgg ctgcggtgcg tacatgatgg
     4141 cgtggatgac caacccgatg atgatcgcac ccatgatgac caggtagttg gcactgttca
     4201 tcatcgggaa cgcgaagaag ccccaggtgc ccgcggcgag cgcgccgacg aagtacaccg
     4261 gtcgacgccc gacgcgatca gacaacctgc ccaccaacgg aatcacgacg aaatgcaccg
     4321 catgcgcggc cagcagccac cacaggatgt cgctggtgtc ggcgccgacc tgcaccttga
     4381 gatacgtgat cgagaaggtg acgaccaggt agtacatgat gttctcggcg aaccgcagac
     4441 ccatcgccgt gaaaacgccg cgcgggtaac gcttcagcac ctcgacgacg ccgtaggaga
     4501 cggccttgac gcgctcggcc tcctgttggg ccgcgacgaa gatcggcgca tcggtcacct
     4561 tggtgcggat gtagtagccg atcagcacaa cgacggcgga cagccagaac gcgacccgcc
     4621 acccccacga caggaacgcg tcatcagaca gcgtcgtcgt caggaccagc agcacgacag
     4681 tggcgagcat gttgccgacg ggcactgcgg cctgcggcca gctggcccag aacgcgcggg
     4741 attggtttgg gctgtgttcg gcgaccagca gcacggcgcc gccccattcg ccgcctaccg
     4801 cgaagccttg gaggaaccgc aacaccacga gcagggccgg cgcccagtag ccgacctgcc
     4861 cgaacgtcgg cagacagccc atcaggaacg tcacggcccc gacgagcagc aggctgaact
     4921 gcaataactt tttgcgaccg tatttgtcgc cgaagtgacc gaacaccaca ccgccgatcg
     4981 ggcgcgcggc gaagccgacg gcgtaggtca ggaatgccgc gaggatcgcg tcgaggtcgc
     5041 tgtcgccctt ggcgaagaac actttgctga acaccaaggt ggcggcggtg ccgtagagga
     5101 agaactcgta ccactcgacg acggtgccgg ccatcgaggc tgcgaccacg cgcttgagcc
     5161 cggtggcgat cggccctggt tcgtcgctta ctggtcgtgt ctcgtctcgg acactcatcg
     5221 cacactcctg cctcagatgt gatggcggac acagatctct gtggagtatt catgcagata
     5281 agcgcacgtg caatggccaa aactgcacga cgtatctgca aaaatgcaga tatgaccgga
     5341 ccctcaggag gccgccgccc gagcgccgac gacctattgg tgctgctggc cgtgggtcga
     5401 tccgggcgct acaacaccgc cgccgaggag cttggcctca accacaccac catctcgcgc
     5461 cgcatcgccg cgctcgaaca gtcgatcggc ggccgagtgc tcgcacgcgt cggcggcggc
     5521 tgggagctca ccgacctcgg ccgcgaggca ctggccgccg ccgaggcggt cgactcggca
     5581 gtccggtcgc tgaccgttga cccaggcggc gtgcgcaccc tcgaaggcgt cgtacggata
     5641 tcggccacag acggattcag cgcgtacatc gccgcgccgg cggcagcaca ggtgcagcgc
     5701 cggcatccca aagtggcagt cgagatcgtg gccgccaccc ggcgggccac ccagcagcgg
     5761 tcaggcctcg acgtcgaggt ggtcgtcggt gaaccgaagg tccatcgcgc caaggcgatt
     5821 cggttgggcg actactgcct tgggctgtac gggtctcgcg actacctctc tgagcacggc
     5881 acgccgacga gcatcgcgga cctcgcacgt tatccgctgg tgtacttcat cgactcgatg
     5941 ttgcaggtcg atgatctcga catcgcgacg agcttcgccc ccgcgatgcg tgaatcagtc
     6001 acgtccacga acgttttcgt ccacgtcgaa gcgacccggg cccacgcagg catcgggatg
     6061 ctgccgtgct tcatggccga ccgacacgac gacctgatcc gcgtactacc cgacgcggtg
     6121 gccatccagt tgacctactg gctggtcact cgcgccgaga cactgcgcag gccggaggtc
     6181 gcggcagtcg tggacgccat ccggcaccgc gtccacgacc agcgcgacgt gctgctcggc
     6241 cggcatcagt gacggcgacg ccgcttgcgc gcttttcgct ggcgacggcg cgatgacgtg
     6301 atcgcgcgag gacgcgtcgg cgccgggcac gtgctggccg acgggaacgt ccgccgtatg
     6361 cacaattcat cgagcgcaag ggcgcggcat gcggtgacca ggtcctcgag gacggtggtc
     6421 atgaccacgg tgttgatcag gccggcgtcg tccagggcta cgatgtcctc tggatccggc
     6481 aattcatatg attcgaacat acgagcgacg gtatagctga cggctgacaa ccaacctgat
     6541 ccgaacgctt gcggttcacc cggcatcttg gtgcagtcgt ggcatgccgt tcatcgatca
     6601 ccccaagggc cgcgcctact tccggcactg ggcggcggtg gagccgcgtg cggccgtcat
     6661 cttcctgcac ggcttcggcg agcacaccgg cgtctatcac cggtacggct tcgcattgaa
     6721 cgccgcaggc atcgacttgt gggccgtcga ccagttcggc cacggcctca gccccggcga
     6781 ccgcggcgac ttcggttcga tcgaagacag ctcgcatctg gctgacagct tgaccgaact
     6841 cgcggagggc gcgcgcagcg gattgccgct gatcgcgcaa gggcattcgt tcggctcggt
     6901 cgtcacgctg ttccggctgc tggaacaacc cgagcgttat cgggcaggga tcatctccgg
     6961 tgcgccgctg gtgccgattc ccgaaatgct cgacgctgac agctctttcg atctggatcc
     7021 cggctggctt tccggcgacc cgttctacat cgactccctt gagaacgacc cgctggcgtt
     7081 cgtcgatgcc gatggcaagc cgctggcgcg cgaactcgac aaggcgtggg accggttcgg
     7141 ctcggagctg cccaaactcg ccgtgccgac gctggcggtg cacggcacca acgatccgat
     7201 cgcaccggtc gacgcggtgc gcgcgtacgc ggatcagcta gatgcgttgc agctcaagga
     7261 attt
//
LOCUS       DASRJA010000335        15573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1193418,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000335 DASRJA010000000
VERSION     DASRJA010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15573
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193418"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VFC01_14700"
     CDS             complement(<1..1025)
                     /locus_tag="VFC01_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80915.1"
                     /translation="MKVIDESGEATDACLPIAPDAQRVDLFMPKFSKPTSITNPLHPT
                     SEVQQTIYGGHVDDKPFRTEVTLFPDTKPIVWQGKTVDTAVSQYVAYLDGRIHEVAID
                     WYAQADDGSVWYFGEDVFNYEDGKVADTNGTWVAGDTTPAAMIMPARPQIGNVYRPEN
                     APGIAFEEVRVDKADLQVDGPSGKISGAIEVNELHMDGTREGKIFAPGYGEFSTGTPG
                     GDLEAASLASPTDRRQGPAPAEFAVLSQAVNDVYAADPSHVRDAVAALSKAWEAVRTK
                     GIPPQIEAQMRVDIEALSSADVQSAALRVAQNELDLRLLYEPVIDVDLARLKLWTRQL
                     PIDVAAK"
     gene            complement(1122..1454)
                     /locus_tag="VFC01_14705"
     CDS             complement(1122..1454)
                     /locus_tag="VFC01_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80916.1"
                     /translation="MNITRIGIATAVAAAAVAIGAGIATAQPDDGEGTPITGEALTKA
                     SQAALAFTGGGTVTATEVGDEESYYEVEVTKADGSQVDVQLDENFVVVGSKTDGPGTD
                     TEPDGDGN"
     gene            complement(1475..2776)
                     /locus_tag="VFC01_14710"
     CDS             complement(1475..2776)
                     /locus_tag="VFC01_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN80917.1"
                     /translation="MATIVVAIALVVGSTGLVAALRRTMVDEVAEAARAQAADVVRQL
                     EAGRPPVLEVAGADEQVIQLMTPAGAVVSSSPNMMGRPAVARLTPGQSAQVVTPLDDD
                     EFVAVAEGAQTPDGLRIVVVARALVDVLDTTAVVTRLLIIGLPVMVVVVALVTWFAVG
                     RALAPVDSIRREVDEISAAQLHRRVPQPKADDEIGRLAATMNRMLQRLESARNSQRRF
                     VSDASHELRSPITTIRHHAEVALAHPDRVTATELAEVVLAEQQRMQHLVDDLLLLARA
                     DEHVPLAREALDLDDLAFEEGRRLRSVTSKRVDTSGVSAARVQGDAEALRRMMRNVGE
                     NAVRHASSRVDIALAERGDEAVLTTDDDGSGIPESERARVLQRFVRLDEARSREAGGS
                     GLGLAIVDEVVRAHGGSVSIEPSPLGGARIRITLPVQAAVS"
     gene            complement(2811..3482)
                     /locus_tag="VFC01_14715"
     CDS             complement(2811..3482)
                     /locus_tag="VFC01_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN80918.1"
                     /translation="MRLLVVEDELRLAAGLRKGLEAEGFAVDVVHNGTDGIWMARENP
                     FDAIILDVMLPGANGYQVCRTLRSEGNWTPILMLTAKDGVWDQVEGLDTGADDYLTKP
                     FSYAVLIARLRALRRRGARARPTVLEAGDLRLDPAARRVWLCGEQIELTRREFAILEY
                     LLHHPDEVMSKRDILEHVWDFDFDGDPNIVEVYVRRLRTKLHRDRPVIETVRGVGYRL
                     VAARD"
     gene            complement(3530..4543)
                     /locus_tag="VFC01_14720"
     CDS             complement(3530..4543)
                     /locus_tag="VFC01_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HZN80919.1"
                     /translation="MAFDFRDLSVPIIVAPMAGGPSTPQMAAAGTNAAGIGFVAAGYL
                     TAEVFAERLAAARNLASGPIGANLFVPQPSAGTAEDIELYAKALGPEAQRYDAQLGEP
                     RWDDDHWAAKIEVLLDVRPEVASFTFGVPSAEECRRLREAGVTVVGTVTTLAEAQTAV
                     ARGVDAVAAQGPSAGGHRGTFDPGAQPATQPLDELLAEVEAAVDLPVVAAGGLMTADD
                     VARVLAAGAVAAQLGTAFLLSAESGSSPVHRAALQDPRFTETVVTRAFSGRYARGLRN
                     RFIDEHEAEAPFGYPEIHWLTSPLRAASVRAGDPQAVNVWAGTGFRNAKAATVADIMA
                     ELT"
     gene            4586..5332
                     /locus_tag="VFC01_14725"
     CDS             4586..5332
                     /locus_tag="VFC01_14725"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN80920.1"
                     /translation="MFSESDGYERFMGRWSRRLAPLFVTFAGVTEGDRVLDVGSGTGA
                     LSAAAAAVDSVQVTGIEPSAAFVRYAREHVDGRFEVGDALALPFSDRAFDRTLSMLVL
                     NFVPDPATALQQMMRVTRPAGVVAAAVWDYGDGMQMLRTFWTAAVGLDPDAAARDERH
                     MPLCTRGSLAQLWRTHGLHGVDEQPLTIDMAFTSFDDYWQPFLCGQGPAGLYTSSLTE
                     SARKVLESKLRADVNLTMRARAWAVRGVIT"
     gene            complement(5325..5855)
                     /locus_tag="VFC01_14730"
     CDS             complement(5325..5855)
                     /locus_tag="VFC01_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN80921.1"
                     /translation="MADVEVLKGPRVTLRAPTLDDAEPLFKRIVSDPEVSRYMSWRPH
                     ADVGETRRVITDIFNVGGETTWLIELRNGGVPIGVCGWRRPQPHIIDFGYFLGRPWWR
                     QGLMSEVVQLLLDKAKRDPTVYRVTAHCHVDNTGSAALLKRSGLTLEGRLARYAVLPN
                     LSPEPQDCLMFGKALR"
     gene            5876..6268
                     /locus_tag="VFC01_14735"
     CDS             5876..6268
                     /locus_tag="VFC01_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN80922.1"
                     /translation="MAITFNHTIVAAKDREESASFFTELFGLPAAKEFGPFLAVELNH
                     GVSLDYAQVAQGEDIRPQHYAFLVSEDEFDAIYGRIRERGMQHWADPRGSRPGEINHN
                     DGGRGVYFQDPGGHYLEILTRPYGSGGG"
     gene            complement(6237..6896)
                     /locus_tag="VFC01_14740"
     CDS             complement(6237..6896)
                     /locus_tag="VFC01_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HZN80923.1"
                     /translation="MSSHDLLRANDARFHALAAGFSADNWSQPSLCDAWTNHDVLAHL
                     VVGYSAGALAVTAEMLRHRGSFDRANAAMARAVAASRAPADLLDDFERLTQRPRGLGR
                     YFPRRLLLGDHITHELDMIFALGREPEIPADALVAVLDTQVRVPNPFVPAFRNSRELR
                     LIATDADWTYGDRGPIVRGCAADLVSVLGSRPARLARLDGDGADMLASRLSRHLSRRG
                     G"
     gene            complement(6883..7785)
                     /locus_tag="VFC01_14745"
     CDS             complement(6883..7785)
                     /locus_tag="VFC01_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN80924.1"
                     /translation="MPATIPTERVDFSCRGTRCAAWLTLPAGSGPHPAVVLVHGLGAT
                     HDMMLAQYEQHFAAAGIGTLAFDYRNTGASGGQPRQHISMRKQRDDVEAALEHVKSQP
                     GIDGGRIGLWGTSLGGMNVARVAAARKDVAVAVVQCPIVHGPGAARKLGLSSALRLTP
                     AIAEDLLRVATKRGRRYVPIVGPPGSLAMVTVDGAESGWRSTVPPGGQFDNRIAAADA
                     VATVMTSATRRARNIDAPLLVCVCDRESLMDSVYAELVARRAPRGVARHYDSDHFAIY
                     HPPLVAQVLADQTAFLQEHLGVFA"
     gene            7867..8505
                     /locus_tag="VFC01_14750"
     CDS             7867..8505
                     /locus_tag="VFC01_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80925.1"
                     /translation="MRARFTTDEIAAAALGIVDAEGLSALSMRSLAAALGTGPMTVYN
                     YVADKEGLEELVVAAVVAQVRLPDPTDDWQHDAYAIADAMWRGIRAHPAAIPLVLTRR
                     TLSATGFAAADALIAALTRAGLPDRDQLGAFHAVLGFVVGAAQAELAGPLTRGRDAAD
                     AAARIGSMAGATYPHIEALSKVAMRTTVEEDFDCGLRMLLDGIAIRRSRQRR"
     gene            complement(8502..8978)
                     /locus_tag="VFC01_14755"
     CDS             complement(8502..8978)
                     /locus_tag="VFC01_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HZN80926.1"
                     /translation="MRMSAKAEYAVRAMVQLATFDEGVLVKTDDVAKAQGIPPQFLVD
                     ILSDLRTDRLVRSHRGRDGGYELARPAADISIADVLRCIDGPLASVRDIGLGDLPYSG
                     PTAALTDVWRALRASMRSVLEETSLADVAAGALPTHVAKMADDYLGQEERRGHGVG"
     gene            complement(8989..9585)
                     /locus_tag="VFC01_14760"
     CDS             complement(8989..9585)
                     /locus_tag="VFC01_14760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HZN80927.1"
                     /translation="MTNPTLRVLADLPQLPVSLSESTLILIDCQNTYTRGVMELEGVQ
                     EALDEAAVLLDRARSAGIPIIHIQHDDGPGSLYDIEGESGAIVSRVAPHNGEPVVVKN
                     YPNSFVQTDLDEVLKTANASNLVLAGFMTHMCVNSTARGAFNLGYAPTVVASATATRS
                     LPGLGDAPVSAEAMQAASLAALADLFAVVVPNANDIPD"
     gene            complement(9609..10340)
                     /locus_tag="VFC01_14765"
     CDS             complement(9609..10340)
                     /locus_tag="VFC01_14765"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HZN80928.1"
                     /translation="MSDHHVAARLATEAGKLLLDVRAELADASEAGRKAAGDKRSHDF
                     LIEALGTERPGDAVLSEEGADNPVRLTAERVWIVDPLDGTREFSELGREDWAVHVALW
                     QSGELVAGAVALPAQGITLATPTVNPPPATADKPRIVVSRTRPPAIALAVRDALDGTL
                     VEMGSAGAKVASVIQGLSDIYVHAGGQYEWDSAAPVAVARAAGLHTSRIDGSSLAYNQ
                     ADPLLPDLVVCRPEFADAVLAVTAG"
     gene            complement(10337..12181)
                     /gene="cysN"
                     /locus_tag="VFC01_14770"
     CDS             complement(10337..12181)
                     /gene="cysN"
                     /locus_tag="VFC01_14770"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HZN80929.1"
                     /translation="MTTLLRLATAGSVDDGKSTLIGRLLYDSKAVMEDQLASVERTSK
                     ERGHDYTDLALVTDGLRAEREQGITIDVAYRYFATPKRKFIIADTPGHVQYTRNMVTG
                     ASTAQLAIVLVDARNGLLEQSRRHTFLASLLGIRHIVLAVNKMDLIGWDREQFEKIRD
                     DFHEFAARLDIHDVTTIPLSALLGDNVVTKSENTPWYDGPSLLSHLEEVYIAGDRNLV
                     DVRFPVQYVIRPQSLDHHDHRSYAGTVASGVMRPGDEVVVLPTGKTSRITEIEGPSGP
                     VQEAFPPMAVSVSLADDIDISRGDLIARPNNQPRVDQEFDATVCWMADESSLEPGRDY
                     IIKHTTRTTRAKVTALDYRLDVNTLHRDKTATALKLNELGRISLRTQVPLLLDEYSRN
                     AATGSFILIDPHTNGTVAAGMILPFVTARTSSPNTVRHESLCKAEDRLSKGRTVWFTG
                     LSGSGKSSVAMLVEQKLLEKGTPAYVLDGDNLRHGLNADLGFSMADRAENQRRLAHIA
                     AILADSGQVVLVPAISPLQEHRALARQVTIEAGLDFFEVFCDTPLEDCERRDPKGLYA
                     KARAGEITHFTGIDSPYQRPKKPDLRLTPDRTTDELADLVIELLESRR"
     gene            complement(12181..13107)
                     /gene="cysD"
                     /locus_tag="VFC01_14775"
     CDS             complement(12181..13107)
                     /gene="cysD"
                     /locus_tag="VFC01_14775"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HZN80930.1"
                     /translation="MTAAQELNQAGRYELSHLRSLEAEAIHIIREVAAEFERPVLLFS
                     GGKDSIVMLHLALKAFQPGRLPFPVMHVDTGHNFDEVLQTRDELVEEAGVRLIVAKVQ
                     DDIDAGRVVETIPSRNPLQTVTLLRAIRENKFDAAFGGARRDEEKARAKERVFSFRDE
                     FGQWDPKAQRPELWNLYNGRHHKGEHIRVFPLSNWTEFDIWSYIGAEKIKLPSIYYAH
                     PRNVFQRDGMLLAVHRYLQPRKDEEVVEKTVRFRTVGDVTCTGCVESLAGTVSEVIAE
                     TAVSRLTERGATRADDRISEAGMEDRKREGYF"
     gene            13268..14536
                     /locus_tag="VFC01_14780"
     CDS             13268..14536
                     /locus_tag="VFC01_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemopexin repeat-containing protein"
                     /protein_id="HZN80931.1"
                     /translation="MSKLVSLGWNDEKAYFFSGGQYVRWDVASDTVDVGYPRPISSGW
                     IGIFPDGIDAAAVWNNGKAYFFRGAEYSAYDIAADVVVDGYPRTIAENWPGVFPEGVD
                     AVIVWGNGKAYFFRGDEYLSYDMAADTVDDGYPRPIRDGWVQPFDTGIDSAVVWNNGK
                     AYFFRGDEYVRYDVATEMADEGYPQPISLGWPGARGERANPSRTSPTPHASEHGVHAS
                     VRDYFLAFTEPLEGRIYCMYLDIEGYVTTAVGNLIDTEGDACRLPWTTADGTRAAESE
                     IRSEWRLVKGMTELARQGAGAAARATQLRLTDAAIDDLVLAKFDANTPVLRRAYADWD
                     SWPADAQLGCHSILWTGSRFPDDKYWPRFNAAVKTRDWLTAADQSHINDEANPGVTAR
                     NTANHELFKNASRVDAQGLDITALVYRHQR"
     gene            14568..>15573
                     /locus_tag="VFC01_14785"
     CDS             14568..>15573
                     /locus_tag="VFC01_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemopexin repeat-containing protein"
                     /protein_id="HZN80932.1"
                     /translation="MSKLVGMMWNDDTAYFFRGGQYVRYSVSADSVDGGYPLLISSGW
                     KDIFTEDIDAAGVWNNGRAYFFKGSQYCAYDVVADAAVEGYPRSIAQNWPGVFPDAID
                     AVLPWGDGKAYFFRGDQYLRYDMATDTVDEGYPKPIADGWSHYFTEDIDSAVRWPNGK
                     AYFFKEDQYLAFDIARDVVDAGYPVSLGDWPGPLGASGSGGTAETMSARRSKIIDELL
                     PGVLPSKYKEQKFIKLTFGLTKESPGVTAGYTTCGSLPMYIARMLGDKIITGTNGVRT
                     AGVNEGAWVEAGDGARPKPGDLYALLNKDSTDRARGGISHVGVIVDASTDEWKTADAG
                     Q"
BASE COUNT         2546 a         5259 c         5150 g         2618 t
ORIGIN      
        1 tccttcgccg ccacatcgat cggcagctga cgcgtccaca acttcagccg cgccaggtcc
       61 acgtcgatga caggttcgta cagcagccgc agatccagct cgttctgcgc gacgcgcaag
      121 gccgccgact gtacatccgc cgagctcagc gcctcgatgt cgacccgcat ctgcgcctct
      181 atctgcggtg gaattccctt ggtgcgcact gcctcccacg ccttgctcaa cgccgccacg
      241 gcgtccctca cgtgactggg gtcggccgca tagacatcgt taactgcctg cgatagcacc
      301 gcgaactcgg cgggcgcagg accttgccgg cggtcggtgg gcgatgccag cgatgcggcc
      361 tcaagatcgc cgccgggcgt gccggtagag aactcgccgt agcccggtgc gaagatcttg
      421 ccctcgcgtg tgccgtccat gtgcagttca ttgacttcga ttgcgccact gatctttccg
      481 ctgggaccgt cgacttgcag gtctgccttg tcgacgcgaa cctcctcgaa cgcgatcccc
      541 ggcgcgttct ccggccgata cacattcccg atctgcggcc tggccggcat gatcatcgca
      601 gccggggtcg tgtcgccggc cacccaggtg ccgttggtgt cggcgacctt gccatcctcg
      661 tagttgaaga cgtcctcgcc gaagtaccac accgatccgt cgtcggcttg cgcataccag
      721 tcgatggcca cctcgtggat ccggccgtca aggtatgcga cgtattggct gaccgccgtg
      781 tcgacggttt tcccttgcca cacaatcggc ttggtgtccg gaaagagggt gacttcggtg
      841 cggaacggtt tgtcgtcgac gtggccgccg tagatggtct gctgcacctc gctagtgggg
      901 tgcaacgggt tggtgatcga cgtgggcttg ctgaacttcg gcatgaacag gtcgacgcgc
      961 tgcgcatcgg gcgcgatggg aaggcacgcg tcggtggcct cgcccgactc gtcgatcacc
     1021 ttcatgcact ctgctgactc gacgggcttc tcatcgctgc acgcggcagc cacgagcgca
     1081 cacgcgagta cggcggtgag acggcggccg accatgaggc cctagtttcc gtcgccgtcg
     1141 ggttccgtgt cggtgccagg tccgtcggtc ttgctaccga cgaccacgaa gttctcgtcg
     1201 agctggacgt cgacctgact gccgtcggcc ttggtcacct cgacctcgta gtagctctcc
     1261 tcgtcgccga cctcggttgc agtgaccgtt ccgccgccgg tgaacgccag cgcggcctga
     1321 cttgccttcg tcagcgcctc tccggtgata ggagtgccct cgccgtcatc gggctgtgcg
     1381 gttgcgatgc cggcgccgat ggcgaccgcc gccgctgcga ctgccgtcgc gatcccgatc
     1441 cgtgtgatgt tcatgcgcgc aaggttgccc cgatctagct gacagcagcc tgaacgggca
     1501 gcgtgatccg gatccgggca ccgccgagcg gggacggctc gatcgacacc gagccgccgt
     1561 gggcccgaac cacctcgtcg acgatcgcga gccccagccc gctgccgccc gcctcgcggg
     1621 agcgggcttc gtcgaggcgt acgaatcgct gcagcacccg ggcccgctcg gactccggaa
     1681 tgcccgagcc gtcgtcgtcg gtcgtgagca ccgcctcgtc gccgcgctcg gcgagcgcga
     1741 tgtccacccg gctcgacgcg tgccggacgg cgttctcgcc gacgttgcgc atcatccgcc
     1801 gcagcgcctc ggcatcgccc tgcacccgag ccgcgctgac ccccgacgtg tcgacccgct
     1861 tcgacgtgac ggagcgcagc cgacggccct cctcaaaggc gaggtcgtcg aggtcgagcg
     1921 cctcacgtgc cagcgggacg tgctcgtcgg cgcgggccag cagcaacagg tcatcgacga
     1981 ggtgctgcat ccgttgctgt tcggccagca cgacctcggc gagttcagtg gccgtgaccc
     2041 ggtcaggatg ggccagcgcg acctcggcat ggtgcctgat ggtcgtgatc ggtgaacgaa
     2101 gttcgtgcga cgcgtcggat acgaagcggc gctggctgtt tcgcgcactc tccaagcgct
     2161 gcagcatccg gttcatagtt gcggccaatc ggccgatctc gtcgtcggcc ttgggctgcg
     2221 gcacgcgccg atgcagttgc gcggccgaga tctcatccac ctcgcggcgg atggagtcga
     2281 ccggtgccag cgcgcgaccg acggcgaacc atgtgacaag cgcgaccacc acgaccatga
     2341 ccggcagccc gatgatcagc aggcgggtca cgacggcggt ggtgtcgagg acatcgacca
     2401 acgccctggc gaccaccacg attcgcaatc cgtcgggcgt ctgcgcgccc tcggccaccg
     2461 cgacgaactc gtcgtcgtcg agtggggtga cgacctgggc ggactggccg ggcgtcaatc
     2521 gcgcaacggc aggcctgccc atcatgttcg ggctcgacga aaccaccgcc ccggccggcg
     2581 tcatcagctg aatcacctgc tcgtccgcgc ccgccacctc aaggaccggc ggccggcctg
     2641 cctcgagttg acgcaccaca tcggcggcct gggctcgtgc ggcctcggcc acctcgtcga
     2701 ccatcgtgcg acgcaacgcg gcgacgagcc cggtcgagcc gaccacgagg gcgatcgcga
     2761 cgactatcgt cgccacggcg gtggtcctcg cccggatcgt cgcgagtcgg ttagtcacga
     2821 gcagcgacca gccggtaccc gacacccctg accgtctcga tgacgggccg gtcccggtga
     2881 agcttggtgc gaagtctgcg cacatatacc tcgacgatgt tgggatcgcc gtcgaagtcg
     2941 aaatcccata cgtgctcaag gatgtcgcgc ttcgacatca cctcgtcggg atggtgcagc
     3001 aggtactcga ggatcgcgaa ctcgcggcgg gtgagctcga tctgttcgcc gcaaagccac
     3061 acccggcgcg ccgccggatc cagccgcaag tcccccgcct cgagcaccgt cggcctggcg
     3121 cgcgcgcctc ggcggcgcag tgcccgcagc cgcgcgatca gcaccgcata cgagaacggc
     3181 ttggtcagat agtcgtcggc cccggtgtcc agaccttcga cctgatccca gacaccgtcc
     3241 ttcgcggtca gcatcaggat gggcgtccaa ttgccttcgc tgcgcagcgt ccggcagacc
     3301 tggtagccgt tcgcgccggg cagcatcacg tcgagaatga tcgcgtcgaa cgggttttcg
     3361 cgcgccatcc agatgccgtc ggtgccgttg tggacgacat cgacggcgaa gccttcggcc
     3421 tcgagcccct ttcgcagccc ggcggccagg cgcagttcgt cttcgacgac caagagacgc
     3481 atcctggctg ccatgatcgc agccagaaac tgactggcgg ctgaattcgc taggtcagct
     3541 cggccatgat gtcggccacc gtggccgcct tggcgttgcg gaatccggtg cccgcccaaa
     3601 cgttcacggc ctgcgggtcg cccgcccgca ccgatgctgc ccgcaatgga ctggtcagcc
     3661 agtgaatctc cggatacccg aacggagcct cggcctcgtg ctcgtcaatg aaccggttac
     3721 gcagcccgcg tgcgtagcgg ccggaaaacg cccgcgtgac aacggtttcg gtgaatcgcg
     3781 gatcctgtag tgcagcgcgg tgcacggggc tgctgcccga ttcggccgac agcaggaacg
     3841 ccgttcccag ctgggcggcg accgctcctg ccgccaacac gcgcgcgacg tcgtctgcgg
     3901 tcatcaaccc gcccgcagcc acaacgggca aatcgactgc ggcttccacc tctgccagca
     3961 gttcgtcgag gggttgggtg gccggctgcg cacccggatc gaaggtgccg cgatgcccac
     4021 ccgccgatgg gccctgcgcc gccaccgcgt cgacgccccg ggcaacagcg gtctgcgcct
     4081 cggcaagcgt ggtcaccgtg ccgacgaccg tgacgcctgc ctcgcgcagc cgacggcact
     4141 cctcggcgct cggcacaccg aacgtgaacg atgcgacctc cggccgcacg tcgagcagca
     4201 cctcgatctt ggccgcccag tggtcgtcgt cccaccgcgg ctcaccgagc tgtgcgtcgt
     4261 agcgttgcgc ctccggcccg agcgccttgg cgtaaagttc gatgtcctcc gcggtgcccg
     4321 cactcggttg tggcacaaac agattcgcgc cgatcgggcc cgatgcgaga ttgcgcgcgg
     4381 cggcgagccg ctcggcgaac acctccgcag tcagataccc cgcggcgacg aacccgatgc
     4441 cggcggcatt cgtccctgcc gccgccattt gcggcgtcga cgggccaccg gccatcggcg
     4501 cgacgatgat cgggacagaa agatcgcgaa agtcgaacgc catcggtctt tcgtatcacg
     4561 gccgcagaga tagcctgacc acaggatgtt ctccgaaagc gacggctacg agcggttcat
     4621 gggccgctgg agccgtcggc tcgccccgct gttcgtgacg ttcgcaggcg tcaccgaggg
     4681 cgaccgcgtg ctcgacgtgg ggtcgggcac cggcgcgcta tccgcggccg ccgcggccgt
     4741 cgactcggtg caggtgacag gcatcgagcc gtcagcagcg ttcgttcggt acgcgcggga
     4801 gcatgtggac ggccgcttcg aggtcggcga cgcgctcgcg ctgcccttct ccgaccgtgc
     4861 cttcgaccgg acgctgtcga tgctggtgct gaacttcgtg ccggatccgg ccaccgcgct
     4921 gcagcagatg atgcgcgtca cccgtcccgc cggcgtcgtg gccgcggcgg tgtgggacta
     4981 cggcgacggc atgcagatgc tgcggacgtt ttggaccgcg gccgtagggc tcgatccgga
     5041 cgcggcggca cgcgacgaac ggcacatgcc gctatgcacg cgcggttcgc tcgcgcagct
     5101 gtggcgcacg cacggactgc atggggtcga cgagcaaccg ctgaccatcg acatggcatt
     5161 cacgtcgttc gacgactact ggcagccgtt cttatgcggt cagggacccg cgggtctgta
     5221 cacgtcctcg ttgaccgaat ccgcccgcaa agttttggaa tccaagttgc gcgcagatgt
     5281 gaatttgacg atgcgggcgc gggcatgggc agtgcgcggc gtcatcacct gagcgccttg
     5341 ccgaacatca gacaatcctg cggctccggg ctgaggttcg gtaacaccgc gtagcgcgcg
     5401 aggcggcctt cgagggtcag cccgctgcgc ttcagtaacg cagccgaccc agtgttgtcg
     5461 acgtggcagt gcgccgtcac ccggtacacg gttgggtcgc gcttggcctt gtccagtagt
     5521 agttgcacaa cctcggacat aagcccctgc cgccaccatg gccggcccag gaagtagccg
     5581 aagtcgatga tgtgcggttg cggccgtcgc cagccgcaaa ccccgatcgg cacaccccca
     5641 ttgcgcagtt cgatgagcca ggtggtctca cccccgacgt tgaagatgtc ggtgatcacc
     5701 cgccgtgtct caccgacgtc ggcatgcggg cgccacgaca tgtagcgcga aacttccggg
     5761 tccgacacga ttcgcttgaa cagcggctct gcgtcatcga gtgtgggcgc gcgcaacgtg
     5821 actcttggac ctttgagtac ttcgacgtcc gccatgagtt ctatccttga ttgatatggc
     5881 aatcacgttc aaccacacca tcgtggccgc gaaggaccgc gaggaatctg ccagcttctt
     5941 taccgaatta ttcggcctgc cagccgccaa ggaattcggt cctttcctgg cggtcgaact
     6001 caaccacggt gtcagcctcg actacgcgca ggtggcccag ggtgaggaca tccgcccgca
     6061 gcactacgcg ttcctagtgt ccgaggacga gttcgacgcg atctatgggc gcattcgcga
     6121 gcggggcatg cagcactggg ccgatccgcg gggcagcagg cccggcgaaa tcaaccacaa
     6181 cgacggcggt cgcggcgtgt acttccagga tcccggcggg cactacctgg aaatcctcac
     6241 ccgcccctac ggctcaggtg gcggctgagc cgcgacgcca gcatgtcggc tccgtcgccg
     6301 tccagacggg ccagcctggc cggcctgctg ccaagcacgg acaccagatc ggccgcgcag
     6361 ccgcgcacga tcggacctcg gtcaccgtag gtccagtcgg cgtcggtggc gatcaggcgt
     6421 aactctcggc tgttgcgaaa cgcgggcacg aacggattcg gcacgcgcac ttgggtgtcc
     6481 agcacggcga cgagggcgtc ggccggaatc tcgggttcgc gaccgagcgc gaaaatcatg
     6541 tcgagttcgt gggtgatgtg atcgccgagc agcagccgcc gcgggaagta gcggcccagt
     6601 cctcgcggcc gctgggtcaa ccgctcaaag tcgtcgagca gatccgccgg cgcacgcgat
     6661 gcggccacgg cgcgggccat cgcggcgttc gctcggtcga acgacccacg gtgacgcagc
     6721 atttcggcgg tcaccgccag cgctccggcg ctgtagccga cgaccaagtg cgccagcacg
     6781 tcatggttgg tccaggcgtc gcacaggctc ggttgcgacc aattgtcggc gctgaacccg
     6841 gccgccagcg cgtggaagcg ggcgtcgttg gcccgcagca gatcatgcga agacaccgag
     6901 atgttcctga agaaaggcgg tctggtcggc cagtacttgg gccacgagcg gcgggtggta
     6961 gatcgcaaaa tggtcggagt cgtagtgccg ggcgacgcca cggggtgccc ggcgcgcgac
     7021 cagctcggcg tataccgaat ccatcaggct ctccctgtca cacacgcaca cgagcaacgg
     7081 tgcatcgatg ttgcgggcgc gccgcgtggc cgacgtcatg accgtcgcga cggcgtccgc
     7141 ggcggcgatc cgattgtcga actgcccacc cggcggaacc gttgagcgcc aaccggattc
     7201 cgctccgtcg acggtgacca tcgcgaggct gcccggcggg ccgacgatcg gcacgtaccg
     7261 cctaccgcgt ttcgtggcca cccgcagcag gtcctcggcg atggcgggcg tcagccgcaa
     7321 cgcggatgac agacccagct tccgggccgc gcccggtccg tgcacgatcg ggcattgcac
     7381 caccgcgacc gcgacatcct tccgcgccgc agccacccgg gccacgttca tgccaccgag
     7441 gctggtgccc cacaacccga tgcgaccgcc gtcaatcccc ggctgcgact tgacgtgttc
     7501 cagtgccgcc tcgacgtcgt cacgctgttt gcgcatcgag atgtgttggc gcggttggcc
     7561 acccgacgct ccggtgttgc ggtagtcgaa cgccagcgtg ccgattccgg ccgcggcgaa
     7621 gtgctgttcg tattgcgcca gcatcatgtc gtgcgtcgcg ccgagtccgt gtacgagcac
     7681 gacggccgga tgtggccccg acccggcagg caacgtcagc cacgccgcgc acctggtgcc
     7741 gcggcacgag aaatcgaccc gctcggtcgg aatggtcgcc ggcatctgcg cctccttgga
     7801 taagtacggt gtacgtattc gctacactgc tttacgtacg ctgtacttgt caagggggtc
     7861 agaaaaatgc gcgctcggtt cacgaccgac gagatcgccg cggcggcgct gggcattgtc
     7921 gacgccgagg gactgagcgc gctcagcatg cggtcgttgg cggccgcgtt gggcaccggg
     7981 ccgatgacgg tctacaacta cgtggccgac aaggaagggc tcgaggaact cgtcgtcgcg
     8041 gcggtggtcg cgcaggtgcg cctgcccgac ccgaccgacg attggcagca cgacgcgtac
     8101 gcgattgccg acgcgatgtg gcggggtatc cgtgcgcatc ccgccgcgat tcccctcgtg
     8161 ctgacccgcc gcacgttgtc ggcgacgggt ttcgcggcag ccgacgcact gatcgccgcg
     8221 ctgacgcggg ccggcttgcc cgaccgcgat cagcttggcg cgttccacgc cgtactgggt
     8281 ttcgtggtcg gcgccgcgca ggccgagttg gccggcccgc tcacccgcgg ccgcgatgcg
     8341 gccgacgcgg ccgcgcggat cgggtcgatg gcgggcgcaa cgtacccaca catcgaggcg
     8401 ctctcgaagg tcgcgatgcg cacgaccgtg gaagaagact tcgactgcgg gctgcgcatg
     8461 ctgctcgacg ggatcgcgat ccgccgatca cgtcagcgcc gctagccgac gccgtggccg
     8521 cgccgctctt cctggccgag gtagtcgtcg gccattttcg cgacgtgggt gggcagcgcg
     8581 cctgcggcga cgtcggcgag gctggtctct tcgagcacgg accgcatgct ggcccgcagt
     8641 gcacgccaca catccgtcag cgcagcggtc ggacccgagt acggcaggtc gccgagcccg
     8701 atgtcgcgca cgctggccag cggtccgtcg atgcagcgca gcacgtcggc gatgctgatg
     8761 tcggcagcgg gccgggcgag ctcatatccg ccgtcgcggc cgcggtggct gcgcaccagc
     8821 cggtcggtgc gcagatcgga caggatgtcg acgagaaact gcgggggaat gccctgggcc
     8881 ttggccacgt cgtcggtctt gacgaggaca ccttcgtcga aggtggccag ctgcaccatg
     8941 gccctgacgg catactccgc cttggccgac atcctcatgt tggccagatt agtcgggaat
     9001 atcgtttgcg ttgggaacca cgacggcgaa caggtcggcc aacgcagcga ggctggcggc
     9061 ctgcatcgcc tccgcggaca ccggggcatc acccaatccg ggcaaggagc gggtcgccgt
     9121 cgccgaggcg acgaccgtcg gcgcatagcc gaggttgaac gcgccgcgcg cggtggagtt
     9181 cacgcacatg tgtgtcatga agccggctag cacgaggttc gaggcgttgg cggtcttcaa
     9241 cacctcgtca agatccgtct gcacgaacga gttcggatag ttcttcacca ccaccggctc
     9301 gccgttatgc ggggccaccc gggacacgat cgcaccgctc tccccctcga tgtcatacag
     9361 cgacccgggc ccgtcgtcgt gctggatgtg gatgatcgga atgcccgccg accgtgcccg
     9421 gtccagcaac acggcagcct cgtcgagcgc ttcttgcact ccctcgagct ccatcactcc
     9481 gcgcgtgtac gtgttctggc agtcgatcag gatcagcgtc gattcggaca ggctgaccgg
     9541 cagttgcggc aggtcggcca ggacgcgcag ggtcggattg gtcacgcgcc cgagcgtact
     9601 gtgcggcatc agcccgcggt caccgccagc accgcatcgg cgaactctgg gcggcacacc
     9661 acgagatccg gcagcagcgg gtcggcctgg ttgtaggcca gcgacgaacc atcgatgcgc
     9721 gaagtgtgca gtccggccgc gcgggccacg gccaccggcg cggccgagtc ccactcgtac
     9781 tgaccgcccg catggacata gatgtcggag agcccctgga ttacggacgc cacctttgcg
     9841 ccggccgaac ccatttcgac cagcgtgccg tcgagtgcgt cacgcacagc cagggcgatg
     9901 gccggcggcc gggtgcgcga cacgacgatg cggggcttgt ccgctgtggc cggcggtgga
     9961 ttgacggtcg gggtggcgag cgtaatgcct tgtgccggaa gggcaaccgc gcctgcgacc
    10021 aattcgcctg attgccacag cgcgacgtgc accgcccaat cctcacggcc gagttcggag
    10081 aactcgcggg tgccgtcgag cgggtcgaca atccacaccc gctcggcggt caatcgcacg
    10141 gggttgtcag cgccctcctc ggacagcacc gcatcgccgg gccgctcggt gcccagcgcc
    10201 tcgatcaaga agtcgtggga ccgcttgtcc cccgcggctt tccgcccggc ttcgctcgcg
    10261 tcggccagct cggcgcgcac gtcaagcagc agcttgcccg cctccgtcgc caaccgcgcg
    10321 gcgacgtggt gatcgctcac cgcctgcttt ccagcagctc gatcaccagg tcggcgagct
    10381 cgtcggtcgt gcggtccggt gtcagccgca gatcgggttt cttcggccgt tgatacgggc
    10441 tgtcgatccc ggtgaaatga gtgatctcac cggcccgcgc cttggcgtac aagcccttcg
    10501 ggtcccgtcg ttcgcagtcc tccagcgggg tgtcgcagaa cacctcgaag aagtcgaggc
    10561 ccgcctcgat ggtcacctga cgcgccaacg cgcggtgctc ctgcagcggg ctgatcgcgg
    10621 gaaccagcac gacctggcca gagtcggcga gaatggccgc gatgtgggcc agcctgcgct
    10681 ggttctcggc gcggtcggcc atggagaacc cgaggtcggc attgagaccg tgccgcaagt
    10741 tgtctccgtc gagaacataa gcgggggttc ccttttcgag cagcttctgc tcgacgagca
    10801 tcgccaccga cgacttgccc gaacccgaca acccggtgaa ccacacggtc cggcccttcg
    10861 acagccggtc ctcggctttg cacagcgatt cgtggcgcac cgtgttgggg ctcgacgtgc
    10921 gcgcggtgac gaacggcaag atcatgccag ccgcgactgt accgttggtg tgcgggtcga
    10981 tcaggatgaa cgaacccgtc gccgcgttac ggctgtattc gtcgagcagc agcggcacct
    11041 gggtgcgcag cgagatgcgg cccagctcat tgagtttgag tgcggtcgcg gtcttgtcgc
    11101 gatgcagcgt gttgacatcg aggcgatagt ccagcgccgt caccttggcc cgcgtggtgc
    11161 gggtcgtgtg cttgatgatg tagtcgcggc ccggctccag tgaggattcg tcggccatcc
    11221 agcacaccgt ggcgtcgaac tcctggtcca ccctcggctg attgttcggc cgcgcgatca
    11281 gatcacccct ggagatgtcg atgtcatcgg ctaggctgac cgacaccgcc atcggcggaa
    11341 aggcctcctg gacaggcccg ctcgggccct cgatctcagt gatgcggctt gtcttcccag
    11401 tgggcagcac gacgacctcg tcgccgggac gcatcacacc gctggccacg gtgcccgcgt
    11461 agctgcggtg gtcgtgatgg tcgagggact gcggccggat cacgtactga acagggaacc
    11521 gcacgtcgac gaggttgcgg tcgcccgcga tgtacacctc ttcgagatgg gacagcagcg
    11581 acggtccgtc gtaccaagga gtgttctccg acttggtgac gacgttgtcg cccaacagcg
    11641 ccgacaacgg aatcgtcgtc acgtcatgga tatcgagccg ggcggcgaac tcgtgaaagt
    11701 cgtcgcggat cttctcgaat tgctcacggt cccaaccgat gaggtccatc ttgttgaccg
    11761 cgagcacgat gtgccgaatg cccagcagtg acgccaggaa tgtgtggcgg cgggactgct
    11821 cgagcagccc attgcgggcg tcgaccaaca cgatcgccag ctgcgcggtc gaagcgccag
    11881 tcaccatgtt gcgggtgtac tggacgtggc ccggcgtgtc ggcgatgatg aatttccgct
    11941 tgggagtagc gaaatagcga tatgcgacat cgatcgtgat gccctgctcg cgctcggcgc
    12001 gcaggccgtc ggtcaccaaa gccaggtcgg tgtagtcgtg tccgcgttcc ttggaagtgc
    12061 gctcgacgga ggccagctgg tcttccatca cggctttcga gtcatacagc agccggccga
    12121 tgagcgtgga cttgccgtcg tcgacggatc cggcggtagc caaacgcaac aacgtcgtca
    12181 tcagaagtag ccctcccgct tgcggtcttc catgccggct tcggagattc ggtcgtcggc
    12241 gcgggtcgcg ccgcgttcgg tcagtcggga caccgcggtc tcggcgatca cctcggacac
    12301 ggtgcccgca agggattcca cgcaaccggt gcaggtgacg tcgccgacgg tgcggaagcg
    12361 cacggtcttc tcgacgacct cctcgtcctt gcgcggctgc aggtagcggt gcaccgccag
    12421 cagcatgccg tcgcgctgaa acacgttgcg cgggtgggcg taatagatcg acggcagttt
    12481 gatcttctcg gcgccgatgt acgaccagat gtcgaactcg gtccagttgg acagcgggaa
    12541 cacccggatg tgctcgccct tgtggtgccg cccgttgtac aggttccaca gctctggccg
    12601 ctgcgccttg gggtcccatt ggccgaactc gtcgcggaag gagaacaccc gctccttggc
    12661 gcgggccttc tcctcgtcgc ggcgggcacc gccgaatgcg gcgtcgaact tgttctcgcg
    12721 gatcgcccgg agcagcgtca ccgtctgcag cggattacgc gacgggatgg tctcgaccac
    12781 gcggccggcg tcgatgtcgt cctgcacctt ggcgacgatc agccgcacgc cggcctcttc
    12841 gacgagctca tcgcgggtct gcagcacctc gtcgaagttg tgcccggtgt cgacgtgcat
    12901 caccgggaac ggcagccggc ccggctggaa cgccttgagg gccagatgca gcatgacgat
    12961 ggagtccttg ccgccggaga acagcagcac cggccgctcg aactccgcgg ccacctcgcg
    13021 aatgatgtgg atggcctcgg cttccagcga gcgcagatgg ctcagctcgt agcggccagc
    13081 ctggttgagc tcctgggcgg cggtcatcgc atcctcgtaa agttggtaga gatgaccaga
    13141 tttatctgca taacccgata tgaaaccagc cccggcctgc gctgtcaacg cgcctagctc
    13201 attgccctga ccgatgtagg cgatttcgcc gatgcggcgg tgtcgggcgt cgtcgtgaga
    13261 tggggcgatg agcaagctcg tttcgttggg gtggaacgac gagaaggcgt atttcttctc
    13321 gggcggtcag tacgtgcgct gggacgtcgc gtcggacacc gtggacgtcg gctatccgcg
    13381 gccgatcagc tccggctgga ttgggatctt tcctgacggc atcgacgccg cagcggtctg
    13441 gaacaacggc aaggcctatt tcttcagggg cgccgagtac agcgcgtacg acatcgcggc
    13501 cgacgtcgtt gtggacggat acccgcgcac gatcgcggag aattggccag gcgtctttcc
    13561 ggagggcgtt gatgcggtca tcgtgtgggg caacgggaag gcgtacttct tccgcggcga
    13621 cgagtacctc agctacgaca tggccgcgga cacggtcgat gatggctacc cgcgaccgat
    13681 ccgggacgga tgggttcagc cgttcgatac gggcatcgac tcagcagtcg tgtggaacaa
    13741 cggcaaggcc tacttcttca gaggtgacga gtacgtccgc tatgacgtag cgaccgagat
    13801 ggccgacgag ggctaccccc agccgatcag cttgggctgg cccggagctc ggggggaacg
    13861 cgcgaacccg tcccgcacct cgccgacgcc gcacgcctcc gagcacgggg tccacgccag
    13921 cgtgcgcgac tacttcctcg ccttcaccga gccactcgag ggacgcatct actgcatgta
    13981 cctcgacatc gagggatacg tcaccacggc cgtcggcaat ctcatcgaca ccgaaggcga
    14041 cgcgtgtcgc ctgccgtgga ccacggccga cggcacccgg gcggccgagt cggagatccg
    14101 gtctgagtgg cggctcgtca aggggatgac ggagctggcc cgccagggcg ccggcgccgc
    14161 ggcgcgcgcc acgcagctgc gcctcaccga tgcggcgatc gacgacctcg tcctggcgaa
    14221 gttcgacgca aacacgcccg tgctacgtcg ggcgtacgcc gactgggact catggcctgc
    14281 cgacgcacag ctgggctgcc acagcatcct gtggaccggg tcgcgcttcc ccgacgacaa
    14341 gtactggccg aggttcaacg ccgctgtcaa gacacgggac tggctgaccg cagccgatca
    14401 gtcccacatc aacgacgagg cgaaccccgg ggtgaccgcc cggaatacgg caaatcacga
    14461 gctcttcaaa aacgcatcaa gggtcgacgc ccaagggctc gacatcaccg cgctcgtcta
    14521 ccggcaccaa cgctgagcaa attcaacacc gatcaaggag caaacacatg agcaagctcg
    14581 tgggcatgat gtggaacgac gacacggcgt acttcttccg cggcggccag tacgtgcgct
    14641 acagcgtgag cgcagattcc gtggacggcg gctatccgtt gctgatcagc tcgggctgga
    14701 aggacatctt caccgaggac atcgatgcgg ccggggtctg gaacaacggc agggcctact
    14761 tcttcaaggg ctcccagtac tgtgcctacg acgtcgttgc cgacgctgcc gtcgagggct
    14821 acccccgcag catcgcgcag aactggcctg gcgtcttccc cgacgcgatc gacgcagtcc
    14881 tgccgtgggg tgacggcaag gcctacttct tccgcggcga ccagtacctg cggtacgaca
    14941 tggccacgga caccgtcgac gagggctacc cgaagccgat cgcggacggc tggtcccatt
    15001 acttcaccga agacatcgac tccgccgtgc gctggccgaa cgggaaggcc tacttcttca
    15061 aggaagatca gtacctagcc ttcgacatcg cccgcgacgt cgtcgacgcg ggataccccg
    15121 tctccctggg tgactggcca ggtccgctgg gagcttccgg ctcggggggc acggccgaga
    15181 ccatgagtgc ccggcggtcc aagatcatcg acgagcttct gcccggcgtg ctgcccagca
    15241 agtacaagga gcagaagttc atcaagctga ccttcgggct caccaaggag agccctggcg
    15301 tgacagcggg atacacgact tgcgggtcgt tgccgatgta catcgcccgg atgctgggcg
    15361 acaagatcat caccgggacc aatggcgtgc gcactgccgg cgtcaacgaa ggcgcatggg
    15421 tcgaggccgg cgatggcgcc cggccgaagc cgggtgatct ctacgcgctg ctcaacaagg
    15481 actcgaccga tcgagcccgg ggcggcatct cccatgtcgg ggtcatcgtc gacgcgtcca
    15541 cagatgagtg gaagaccgcc gacgccggcc aag
//
LOCUS       DASRJA010000336        16005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2460793,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000336 DASRJA010000000
VERSION     DASRJA010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16005
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2460793"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFC01_14790"
     CDS             complement(<1..498)
                     /locus_tag="VFC01_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN80933.1"
                     /translation="MTEKVWFITGTSRGFGREWAIAALERGDKVAATARDTATLDDLA
                     EKYGDALLPIRLDVTDRDADFAAVEQAHDHFGRLDIVVNNAGYGHFGFIEELTEAEAR
                     AQIETNVFGALWVTQAALPFLRAQRSGHIIQVSSIGGIVAFQNLGAYHASKWALEGFS
                     QALAQE"
     gene            568..1260
                     /locus_tag="VFC01_14795"
     CDS             568..1260
                     /locus_tag="VFC01_14795"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HZN80934.1"
                     /translation="MSRASLDKDPHEVASMFDAVARRYDLTNTVLSLGQDRFWRRATR
                     SALRIGPGDKVLDLAAGTAVSTVELGASGAWCVAADFSVGMLAAGAQRDVPKVGADAT
                     KLPFDDGVFDAVTISFGLRNVVDYAAGLREMARVTRPGGRLVVCEFSTPTNRLFATAY
                     KEYLMQALPRIARTVSSNPDAYVYLAESIRAWPDQAELAHRIADAGWSAVRWRNLTGG
                     IVALHAATKPLG"
     gene            1301..2356
                     /locus_tag="VFC01_14800"
     CDS             1301..2356
                     /locus_tag="VFC01_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HZN80935.1"
                     /translation="MSYGRLADPDSTLGTDTRSDPRMVKLFAQFGLDGHPPPSPVTAD
                     SPIEDRHAFAAAQEEAITAVFDALVQGIPAIEGVTTTTTTITGEDGNDITLFISRPTD
                     VEGALPCIVHLHGGGMAILSAADPGYMHMREYLAATGLVVVGVEFRNSSGKLGPYPYP
                     AGLNDCAAAIRWVAAHRGDLGVTHLIVSGESGGGNLTLTVAHKAKREGWIHEIAGFYA
                     QCPYISNRWLERCDDLPSLQECDGYFMDCQGLAVMGSLYDPTNSYASDATCWASMATD
                     EELTGLPPHVISVNELDPLRDEGLQYYRRLVHAGVPAVGRVVAGTCHGGDLLFAGAMP
                     EVFAASMRDVSGFAKSL"
     gene            complement(2353..3915)
                     /locus_tag="VFC01_14805"
     CDS             complement(2353..3915)
                     /locus_tag="VFC01_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN80936.1"
                     /translation="MSDYDAIVVGAGHNGLTAAAILQQAGLRTVCLEANTYTGGMAAT
                     VQLIDGFRYEIAGSVQFPMPSQIAKELGLDTLPTVDAEVMSVNLGDEDEEPMIFYRDP
                     MQFMTHLAGKHGTDAVTGMAGLIGWSQGPAKALGRFDVRTPPKTLDEMYACAANEAER
                     TAIHDVIFGSAMDAIDRYLPDKHKHAVLRSMLAFLAINSTYRGPYTPGSATCLAFALA
                     VPDDTTAMMTKLEGGIGALSEHLHQLFVSNGGEVRYRAKVDKILVKDDRVTGVRLHDG
                     SEISAPLVVSNLSPDHTLIELVGAEHLPADLATRLRGRDHRASFVQIHFALDGLPEFA
                     PPYDFLNEPGMQGSIGIFNSPEEQQRQWEECRRGIVPDNPSMGMQIPSVHDPGMAPPG
                     KHAASAFAYALPVENSRDTRKLKNAMAEKVIDKITRFAPNFRDIQIRHITFAPYHMQT
                     MFAAPSGDFCHGLLHPDLMGPNRPGPKGFLDMPVPIDGLYLGGAGCHGGPGITFIPGY
                     NAAYAVLEDASN"
     gene            3974..4573
                     /locus_tag="VFC01_14810"
     CDS             3974..4573
                     /locus_tag="VFC01_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN80937.1"
                     /translation="MTSAPNLHEQRRRSTHEALRRAALASFARKGFANVTVTELAREA
                     GVTERTFFRHFPTKEAVLFQDYETQVEWLSEALAQRPASESLFDGVLASVASFPYDLE
                     VVRQAATARTELISADRIASHLRVVQSSFAGVITDFVKNRYRDTPDIDLVAEVAGACL
                     AAALVVAVENWGRNGCADDLGEITAAALNLVRSGLASLS"
     gene            complement(4856..5302)
                     /locus_tag="VFC01_14815"
     CDS             complement(4856..5302)
                     /locus_tag="VFC01_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HZN80938.1"
                     /translation="MIRPRPALLTIGYQNRTVDDLIAALRAARVKVLVDVRLTPMSRK
                     AGLSKNGLAARLHEAGIDYVHLPALGNPRDNRDAYRRGDEAAVAKYRDVLRTPEGQAA
                     LDQLVRMARHRCVALMCFEHDAAECHRSLVADALVRAGTISQVVHL"
     gene            complement(5299..6354)
                     /locus_tag="VFC01_14820"
     CDS             complement(5299..6354)
                     /locus_tag="VFC01_14820"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZN80939.1"
                     /translation="MNRKEFSVATFNLFNLQEPGKPMNRGQTPWTPEQFRMKIQWIVW
                     QLTILAPDIVGLQELWSKEALDAVLDFNYGTPGDPPKRLKDEYQQLATPATGDKIVCG
                     ALVRKELLVGEPKWVDKFPEATRLDLKEDPDDPQSPEIKVTINAFSRPVLRFQVKLRD
                     DQPVTEVFVTHLKSKLPTDVSEEAWFENNPATFKPHQKALGAGISTIRRTAEAVALRV
                     MLTGVMKGTTTPVIVLGDLNDGQTSNTANILTEQPQILVGGAQGGSDVGLYTAQTLQE
                     YRDTRDVYYTHVHQDLRESLDHVLVSEQFYDNSRKRVWLFDGLVINNDHLNFEDHKGT
                     GTGDHGVVKVSFKFNPA"
     gene            6467..6895
                     /locus_tag="VFC01_14825"
     CDS             6467..6895
                     /locus_tag="VFC01_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80940.1"
                     /translation="MATFWRYVRIQLVVFVFGCVGPIFLGIYFATQPDPAMKWAYWAG
                     LFITAADVLIALGLTNASDPKRAPADARIALAVAKRRQSSSSDQSSSSSTYDPFSSTA
                     FSFSTDSGSSPSDYSSSSSDYSSGDSGSSSSDSGSSSSSD"
     gene            complement(6902..7897)
                     /locus_tag="VFC01_14830"
     CDS             complement(6902..7897)
                     /locus_tag="VFC01_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005058307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN80941.1"
                     /translation="MITRRDFARTVGFAVAATSVASSAIASASPAQSHTSFASLKQVD
                     AAVLNVGYAEDGPADGPPVILLHGWPYDIYSYVDVAPILAARGYRVLIPFLRGYGSTR
                     FLSSDTVRNGQQAALAQDVVALMDALNIDRAVLGGFDWGARTADIVAAIWPERVKALV
                     SVSGYLIVNLVANQQPLSPQAEHGWWYQYYFATDRGVRGYRQNTHDFNKLIWQEASPT
                     WKFDDATYDRSAAAFTNPDHVAIVIDNYRWRLGLSAGETQYDDIDGKLADSPPIAVPT
                     VTIGSDFDGANKDGAAYRKMFTGPYSHRILHGIGHNVPQEAPQAFADAIIAADKM"
     gene            complement(7940..9160)
                     /locus_tag="VFC01_14835"
     CDS             complement(7940..9160)
                     /locus_tag="VFC01_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HZN80942.1"
                     /translation="MDTRADVVVVGAGPAGSAAAAWACRGGRDVLVIDSAQFPRDKAC
                     GDGLTPRAVLELQRLGLGPWLEGRVQHRGLRLVGFGGDVEVDWPGPSFPAVSSAVPRT
                     ELDDRIRMVAVDDGAKMLLGVKAVDVRHDSSGRVSSLLLDSGAEIGCRSLIVADGARS
                     PLGRVLGRQWHQQTVYGVAVRGYIATPRADEPWITSHLELRSPEGRVLPGYGWIFPLG
                     NGEVNIGVGALATSKRPADAALRPLMSYYTSLRRKEWGFEGEPRLALSALLPMGGAVS
                     GVAGPNWMLIGDAAACVNPLNGEGIDYGLETGRLAAEMLGVGDVSSAWPEVLSSHYAR
                     AFSVARRLGLLLTIPRFLPATGPVAMRSAFLMNIAVRVMGNLVTDEDVDWIARVWRSA
                     GLASRRIDRRPPFS"
     gene            9182..10189
                     /gene="grcC1"
                     /locus_tag="VFC01_14840"
     CDS             9182..10189
                     /gene="grcC1"
                     /locus_tag="VFC01_14840"
                     /EC_number="2.5.1.10"
                     /EC_number="2.5.1.29"
                     /EC_number="2.5.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0052924 - all-trans-nonaprenyl-diphosphate
                     synthase (geranylgeranyl-diphosphate specific) activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0033384 - geranyl diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nonaprenyl/(2E,6E)-farnesyl/geranylgeranyl
                     diphosphat synthase"
                     /protein_id="HZN80943.1"
                     /translation="MRTPASVVAGVDFADPVFAQEVRDGVARIEDLMATELGKADELM
                     SEAVQHLFQAGGKRFRPLFTVLSARLGPEPDAWQVSVAGAVIELVHLATLYHDDVMDE
                     AQVRRGAPSANARWSNNIAILAGDYLFATASRLVSRLGPEAVRVIADTFAQLVTGQMR
                     ETRGVAEGVDEVEHYLKVVYEKTACLIAASGRFGATFSGAGEEQIERLSRLGGIVGTA
                     FQISDDIIDIDSDPDESGKVPGTDLREGVHTLPVLYALRETGPDGDRLRQLLKGPVEN
                     DDDLAEALRLLRKSPGMAQAKDTVARYAKQARDELANLPEGPGRQALATLVDYTVNRH
                     G"
     gene            10255..11136
                     /gene="htpX"
                     /locus_tag="VFC01_14845"
     CDS             10255..11136
                     /gene="htpX"
                     /locus_tag="VFC01_14845"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011896072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HZN80944.1"
                     /translation="MTWHPHANRAKTFLLLVGMSALIMFIGALFNSRAILGLAVLFAV
                     GMNVYVYFNSDKLALRAMHAQPVNEMQAPVMYRIVRELATTARQPMPRLYISDTAAPN
                     AFATGRNPRNAAVCCTTGILQILNERELRAVLGHELSHVYNRDILISCVAGAMASVIT
                     ALAFVAHFAGMFGGSRDGGSNPFALLLIALLGPIAATVIRLAVSRSREYQADQSGAEL
                     TGDPLALASALRKISAGVQQAPLPPEPQLADQAHLMIANPFRSGERIGKLFSTHPPME
                     DRIRRLEAMAGRGPGLY"
     gene            11180..11653
                     /locus_tag="VFC01_14850"
     CDS             11180..11653
                     /locus_tag="VFC01_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80945.1"
                     /translation="MLRRILIGVAGIAGAALVGVPGIAAADPPPLPNINAFPSAKPSE
                     YSVNDGARYAFGVLDGVTCVLDKQSGSYGCSGPIPAAPGGANLVSARASGKPGFANAD
                     RPVYGVVEDAKALPPNTRLSFRTVSCGTDGFVTTCLNSIDQSGFVLSPDGSYTFG"
     gene            11688..12230
                     /locus_tag="VFC01_14855"
     CDS             11688..12230
                     /locus_tag="VFC01_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80946.1"
                     /translation="MAVLSRVLAGVLGAVAVLGVPGIAGAEPPPDPPPQPPPPPNVNA
                     LAPVKLSDYATMNDTWYAFTSGGVTCVLQRAGSYGCSGPIPGAPEGANLVSGSYGGVP
                     GFAISPGDVFAAAGAVKPLPAGSRISYQTVSCGNDGATTTCVDGRSQSGFVISPAGSF
                     ILNETPPLLYRPDNRGPFAN"
     gene            12285..12437
                     /locus_tag="VFC01_14860"
     CDS             12285..12437
                     /locus_tag="VFC01_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80947.1"
                     /translation="MAKDHGPSVKNDKQYEGLRKKGMSKARAAKIANTPGASKKGGKK
                     SGKKSS"
     gene            complement(12492..13517)
                     /locus_tag="VFC01_14865"
     CDS             complement(12492..13517)
                     /locus_tag="VFC01_14865"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZN80948.1"
                     /translation="MAPAQREPNVVVLGGGSWGTTVASICARRGPTLQWVRSEETAED
                     INKNHRNTKYLGDDVELSPTLQATNDFSEAANAADVVVMGVPSHGFRGVLEQLAKELR
                     PWVPVVSLVKGLEQGTNYRMSQIVDEVLPGHPAGILAGPNIAREVGEGYAAAAVLAMP
                     DQHLAAKLAELFRTKRFRTYTTDDVVGVEMAGALKNVYAIAVGMGYSLGIGENTRAMV
                     ITRAVREMSKLGEAVGGHRDTFAGLAGTGDLIVTCTSQRSRNRHVGEQLGAGKPIDEI
                     IASMNQVAEGVKASSVIMEFADKYGINMPIAREVDGVINHGSTVEDAYRGLVAEKPGH
                     EVHGSGF"
     gene            13646..14986
                     /locus_tag="VFC01_14870"
     CDS             13646..14986
                     /locus_tag="VFC01_14870"
                     /inference="COORDINATES: protein motif:HMM:NF018018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN80949.1"
                     /translation="MSDPTRVDPQKPAAAVTPTGGSAEEVNAWWGSLGQADRDRLLAE
                     HPSGLGNLNGIPAGIRDQINNAVMNDDLSRIADAAAGHAVSVDDVLASPARYGLSNDD
                     AVRYRNAVQTRRGLDHDRGSDPGNPRPVMLWAYHPSAFHGQGSAAIAIGDPDEAGDIA
                     VIVPGAGSSVAGGWLSGGHNAAINVYDRSIAAYPDEDMSVIAWMGYDAPDNFSDLGIA
                     APLLARAGGELLAQDVNGLWVTHAESNPPHVSVLGHSYGATTVADAFAGSGMRANDAI
                     LLGSPGTDLAKSAADFHVDSGEVYVGAASTDPVSWIGVSGSVPDILNDVFGHPFGPDV
                     GLGADPAGDGFGSVRFEAEVMGRDRLDMGDHSHYYDVGGEALRSIVHIVTGNGGALAR
                     DDLVAEGRRQPHATTPREINIPGLGRISLPHIDTRIPGTPASIDPEAVRGLEST"
     gene            14983..15432
                     /locus_tag="VFC01_14875"
     CDS             14983..15432
                     /locus_tag="VFC01_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80950.1"
                     /translation="MTEDALPRLSDEEAMAQVVEPARQIIRLAGLQDVSGGFLFESCN
                     DQGDPPYRGRVEMSFAMPAGAEPDAYFQQIAAVMLTQGWIDGPPPGKCPIGVVIHTET
                     VMAIIARASAADMRGSVQLCGQCGNITDHRADGKTVGVEITAQLTGS"
     gene            complement(15419..15910)
                     /locus_tag="VFC01_14880"
     CDS             complement(15419..15910)
                     /locus_tag="VFC01_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HZN80951.1"
                     /translation="MADSSFDVVSKVDRQEVDNALNQAAKELATRFDFRGTDTSIAWQ
                     GEETVVITSSTEERIKAAIDVFKEKLIRRDISMKAFDVGDPQASGKTFKVSGAIKQGI
                     SSEDAKKITKLIRDDGPKGVKTQIQGDEVRVSSKKRDDLQAVIAMLKKADLDVALQFV
                     NYR"
BASE COUNT         2714 a         5138 c         5263 g         2890 t
ORIGIN      
        1 ttcttgggcc agcgcctgag agaagccctc gagcgcccac ttcgacgcgt ggtaggcgcc
       61 caggttctgg aacgcgacga tgccgccgat cgacgacacc tggatgatgt ggccgctgcg
      121 ctgtgcccgc aggaacggca gtgcggcctg cgtcacccac agcgcaccga acacgttggt
      181 ctcgatctgg gcgcgcgcct cggcctcggt cagctcctcg atgaagccga aatggccgta
      241 gccggcgttg ttgacgacga tgtccaggcg gccgaagtgg tcgtgcgcct gctcgaccgc
      301 cgcgaagtcg gcgtcgcggt cggtcacatc gagccggatc ggcagcagcg cgtccccgta
      361 cttctcggcg agatcgtcga gtgtcgcggt gtcgcgggcg gtggccgcga ccttgtcgcc
      421 gcgctccagg gccgcgatcg cccactccct gccgaatccg cgtgatgtcc cggtaatgaa
      481 ccaaactttt tcagtcacgc tcgattaatg cccgctatgg cggtcctcat tcccggatta
      541 agcatgttca caggtaccgt cggggccgtg agccgcgcgt cgctggacaa ggacccacat
      601 gaagtggcgt cgatgttcga cgccgtcgcg cgccgctacg acctgaccaa caccgtgctc
      661 tcccttggcc aggaccggtt ctggcggcgc gcgacccggt cggcgctccg gatcgggccg
      721 ggggacaagg ttctcgacct cgcggcgggc acagcggtat cgacggtcga gctgggggcg
      781 tcgggggcgt ggtgtgtcgc ggcggacttc tcggtcggca tgctggcggc aggagcgcag
      841 cgcgatgtac ccaaggtcgg cgctgacgcc accaaattgc ccttcgacga cggcgtattc
      901 gatgcggtga cgatcagctt cgggctgcgc aacgtggtcg actacgcggc gggactgcga
      961 gagatggcac gggtgacgcg cccgggcggg cggttggtgg tgtgcgagtt ctcgacgccg
     1021 acgaaccgat tgttcgcgac tgcctacaag gaatacctga tgcaggcgtt gccccggatc
     1081 gcccgcacgg tgtcgtcgaa tccggacgcc tacgtctatc tggctgagtc gattcgcgcc
     1141 tggcccgatc aggcggaact cgcgcaccgg attgccgacg ccgggtggtc ggcggtgcga
     1201 tggcgcaacc tgaccggcgg catcgtcgcg ctgcacgcgg cgacgaaacc gttgggctaa
     1261 agcacctcag aggcagcgag tccggaggta cggtcgcgcc atgtcctacg gccgcctcgc
     1321 agatcccgat tccacgctcg gcaccgatac ccgctccgac ccgcgcatgg tcaagctgtt
     1381 cgcgcagttc ggtctggacg gccacccgcc gccgtcgccg gtgactgccg actctccgat
     1441 cgaggaccgg catgccttcg ccgctgcgca agaggaggcc ataaccgcgg tcttcgacgc
     1501 tcttgtgcag ggcatcccgg ccatcgaggg cgtcacgaca acgacaacga cgatcactgg
     1561 cgaggacggc aatgacatca ccctgttcat cagccgcccc accgacgtcg agggagcatt
     1621 gccgtgcata gtgcacctcc atggtggagg catggcgatc ctcagcgccg ccgaccccgg
     1681 ctatatgcat atgcgcgaat accttgctgc cacaggactt gtcgttgttg gcgttgaatt
     1741 tcgcaactcc agcggaaagc tcggccccta cccgtatccg gccgggctga acgactgtgc
     1801 ggccgcaatt cgatgggtgg cagcgcatcg cggcgacctc ggtgtgactc acctgatcgt
     1861 gtccggcgaa tccggcggcg gcaacctgac tctcacggtc gcacacaagg ccaagcggga
     1921 aggttggata cacgagatcg ccggtttcta cgcgcagtgc ccgtacatct cgaatcgctg
     1981 gctcgagcgg tgcgatgacc tgccgtcact gcaggagtgc gacggatact tcatggactg
     2041 ccagggcctt gcggtgatgg gctcgctcta cgatcccacc aattcgtacg ccagcgacgc
     2101 cacctgttgg gcgagcatgg ccaccgacga agaactgacc gggctgccgc cgcatgtcat
     2161 ctccgtcaac gaactcgacc cgctgcgcga tgagggcctg cagtactacc ggcgattggt
     2221 gcacgccgga gttcccgcag tgggacgcgt ggtggcaggc acgtgccatg gcggcgactt
     2281 gctgttcgcg ggtgccatgc ccgaggtgtt cgccgcgagt atgcgcgacg tcagcgggtt
     2341 cgcgaagtcg ctctagttcg acgcgtcctc gagcacagcg tatgcggcgt tgtacccggg
     2401 gatgaacgta atccccgggc ccccgtggca tcccgcgccg ccgaggtaga gcccgtcgat
     2461 tggcacaggc atatcgagga atccttttgg gccaggacga ttggggccca tcagatccgg
     2521 gtgcagcaga ccgtggcaga agtcgccgga cggcgcggcg aacattgtct gcatgtgata
     2581 cggcgcgaac gtgatgtggc ggatctggat gtcgcggaag ttcggcgcaa accgggtgat
     2641 cttgtcgatc accttctcgg ccatggcgtt cttgagtttg cgcgtgtcgc gactgttctc
     2701 cacaggcaac gcatatgcga aggcactcgc cgcgtgcttg cccggcggtg ccatgccggg
     2761 atcgtgcacg gacgggatct gcatgcccat cgacgggttg tccgggacga tgccccgcct
     2821 acattcctcc cattgacgct gttgttcttc aggcgaattg aagatcccga tggatccctg
     2881 catgcccggc tcgttgagga agtcgtaggg cggtgcgaac tcgggcagac cgtcgagcgc
     2941 gaagtggatc tgcacaaagg acgcccggtg atcgcggccg cgcaaccgcg tcgcgaggtc
     3001 ggccggcagg tgctcggcgc cgaccagttc gatcaacgtg tgatccggtg acaggttgga
     3061 aaccaccagc ggtgcactga tttccgatcc gtcgtgcaga cgcacaccgg tgacgcgatc
     3121 gtccttgacg aggatcttgt cgaccttggc gcgatacctg acttcaccgc cattggagac
     3181 aaacagctgg tgcaggtgct cgctgagcgc gccgatgccg ccttccagtt tcgtcatcat
     3241 cgcggtggtg tcgtcgggca ccgccagcgc gaacgccaaa cacgttgcgc tgcctggggt
     3301 gtagggaccg cggtaggtgg agttgatggc aaggaaggcg agcatgctgc gcagcacagc
     3361 gtgcttgtgc ttgtcgggca ggtagcggtc gatggcatcc atcgccgatc cgaagatcac
     3421 atcgtggatc gcggtgcgct cggcttcgtt ggcggcgcac gcatacattt cgtcgagcgt
     3481 cttgggtggc gtgcgaacat cgaagcggcc cagtgccttt gccggtccct ggctccagcc
     3541 gatcagcccg gccatccctg tgacggcgtc ggtgccgtgc ttgccggcga ggtgggtcat
     3601 gaattgcatc ggatcccgat agaagatcat cggttcttcg tcttcgtcac cgaggttgac
     3661 cgacatcacc tccgcgtcga ccgtaggcag cgtgtcgagc ccgagctcct tggcgatctg
     3721 gctgggcatg gggaactgca ccgacccagc gatctcgtac cgaaagccat cgatcagctg
     3781 gacggtggcc gccatcccac cggtgtacgt gttcgcctcc aggcagaccg tgcgcagacc
     3841 cgcctgctgc aggatggccg ccgccgtcag cccgttgtga cccgcgccga caacgatcgc
     3901 gtcgtaatcg ctcatcccca aaatatgtca gtactgacat aatttggcaa gggttcgata
     3961 ccatcgccga acgatgactt ccgctccgaa ccttcatgag cagcgccgac gatcgacgca
     4021 tgaagcgctt cggcgcgccg cactggcgag tttcgcgcgc aaaggcttcg ccaacgtgac
     4081 ggtgaccgag cttgcgcggg aggccggggt caccgagcgc accttctttc ggcacttccc
     4141 gaccaaggaa gcggtgctct ttcaggacta cgagacacag gtggagtggc tgtcagaagc
     4201 cttggcgcag cggccggcat cggagtcgct gttcgacggc gtcctggcga gcgtcgcgag
     4261 cttcccgtac gacctcgagg tggtgcgtca ggcggcgacc gcccgaaccg agctgatcag
     4321 tgccgaccgc atcgccagcc acctgcgcgt ggtgcagtcg tcattcgccg gcgtcatcac
     4381 cgacttcgtg aagaaccggt accgcgacac tcccgacatc gacctcgtgg ccgaagtcgc
     4441 gggcgcctgt ctcgccgctg ctcttgttgt ggcagtggag aattggggcc gcaacggctg
     4501 cgcggacgat cttggcgaga tcaccgcggc tgccctcaat ctcgtgaggt ccggactggc
     4561 gtcgttgtcc tgaaccggct agctgacgac gtgaaaaccg ctggtgctgt cagggatgaa
     4621 cgccgtttct ccgctgctct gttgcggcgg agtcggctgc tcgctcgccg gaacgcacac
     4681 accaacgaac ggcacgtctg cgcacgggcc gataggcgtc gcgtttgcaa tgggaatcaa
     4741 gccgaggggg acagttgcgc atgcaatcgc ggcaagcgcc cgaattatca aattgcgtac
     4801 ggtcatcgtg gatccttccg acgactcgtc tgcattcaga gtacgccgct gtcgctcaaa
     4861 ggtggacaac ttgcgatatg gtcccggccc tcaccaacgc atcggcgacc aacgaccgat
     4921 ggcactcggc cgcatcgtgt tcaaaacaca tcaacgccac gcaccgatgt ctagccatac
     4981 gaaccaattg atcaagggcc gcttggcctt ccggtgttct caagacatcg cggtatttcg
     5041 cgacggccgc ctcgtcgccg cgccgataag cgtcacggtt gtcacgcggg ttgccgagcg
     5101 ccggcagatg tacatagtcg attcccgcct cgtgcagccg cgccgcgaga ccgttcttcg
     5161 acaggccggc tttgcgcgac atcggtgtaa gccgcacgtc cacaaggact ttcactcttg
     5221 cggctcgcag cgcggcgatg aggtcgtcga cggtgcggtt ctgataaccg atcgttagca
     5281 aagccggccg cgggcggatc aagccgggtt gaacttgaag ctcactttca ccacaccatg
     5341 gtcaccggtg ccggtccctt tgtggtcctc gaagttgaga tggtcgttgt tgatgaccaa
     5401 accgtcgaac aaccacacgc gcttcctgct gttgtcgtag aactgctcgc tgacgagcac
     5461 gtggtccagc gactcgcgga gatcctgatg cacgtgcgtg tagtacacgt cgcgtgtgtc
     5521 ccggtactcc tgcagcgtct gagccgtata gaggcccaca tcgcttcctc cttgcgcgcc
     5581 gcccacaagg atctgtggct gctcggtgag gatgttcgca gtgttgctgg tctgcccgtc
     5641 gttcaaatcc cccaacacaa tgaccggcgt tgtggtgccc ttcatgacgc cggtgagcat
     5701 gactcgaagt gcgacggcct ccgccgttcg ccggatcgtc gagatgcctg cgccgagcgc
     5761 tttctgatgc ggtttgaacg tcgccggatt gttttcgaac caggcttcct cgctgacgtc
     5821 ggtcggaagc ttggacttga ggtgcgtcac gaacacctcg gttaccggtt ggtcgtctcg
     5881 tagtttgacc tggaaacgca gaacggggcg agagaacgca ttgatagtga ccttgatctc
     5941 cggggactga gggtcgtcgg gatcctcttt caagtccagc cgcgtcgcct cgggaaactt
     6001 gtccacccac ttcggttcac cgactagaag ttctttgcgc accagcgccc cacagacgat
     6061 cttgtcgcca gtcgcaggcg tggcaagctg ttggtactcg tccttcagtc gctttggcgg
     6121 atctcccggg gtgccgtagt tgaagtcgag gacagcgtct agtgcctcct tgctccacag
     6181 ctcctgcagc ccgacgatgt cgggggcgag gatcgtcaac tgccagacga tccactgaat
     6241 cttcatcctg aattgttctg gcgtccaagg tgtttgcccc ctgttcatcg gcttgccagg
     6301 ctcttgcagg ttgaacaggt tgaacgtcgc aacgctgaat tccttgcggt tcatcgatga
     6361 ccccctcgta gtggaatgac ttccgcggtg tcatcctgac gcgaatccgc gacagcgtcg
     6421 tgggtactcg actactcgag atattgcccg cgatatgctg cggcccgtgg ccacattttg
     6481 gcgctacgtc cgcatccagc tggtggtgtt cgttttcggg tgcgtcgggc cgatcttctt
     6541 gggcatttac tttgctacgc aaccggatcc ggccatgaaa tgggcgtatt gggccggact
     6601 gttcatcacc gcggccgacg tgctcatcgc gttgggcctc accaacgcca gcgacccgaa
     6661 aagggcgcct gccgatgccc gcatcgcgtt ggccgtggcc aagcgccgac aatccagcag
     6721 cagcgatcaa tcgagctcgt cgagcaccta cgacccgttt tcgtcgacgg ccttcagctt
     6781 ctcgactgac tctggctcgt cgccgagcga ctactcctcg tcctcgagtg attactcctc
     6841 gggtgattcc ggatcttcat ccagtgattc cggatcgtcg tcgtcgagcg actagccgtc
     6901 gctacatctt gtccgccgcg atgatggcat cggcgaaagc ctgcggcgcc tcctgcggca
     6961 cgttgtgtcc gatgccgtgg aggatgcggt gtgaataggg gcccgtgaac atcttgcggt
     7021 aggcggcacc gtccttgttg gcgccatcga agtcgctgcc gatcgtgacg gtcggcacgg
     7081 cgatcggtgg gctatcggcc agcttgccgt caatgtcgtc gtactgcgtt tcgccggccg
     7141 acaacccgag tcgccagcgg tagttgtcga tgacgatggc gacatgatcc gggttggtga
     7201 acgccgcggc gctgcggtcg taggtggcgt cgtcgaactt ccacgtcggc gacgcctcct
     7261 gccagatgag tttgttgaag tcgtgtgtgt tctggcggta cccgcgaact ccacgatcgg
     7321 tcgcgaaata gtactggtac caccagccgt gttcggcctg cggcgacaaa ggctgttgat
     7381 tggcaacgag attcacgatc aggtagccac tcaccgacac cagcgccttg actctctccg
     7441 gccagatcgc ggcgacaatg tcggcggtcc gtgcgcccca gtcgaatcca ccgagcaccg
     7501 cccggtcgat gttcagcgca tccatgagcg cgacgacgtc ctgcgctagt gccgcctgct
     7561 gtccattacg aacggtgtcc gacgagagga atcgagtgct cccgtagccg cgcaaaaagg
     7621 gaatgagcac ccggtatccg cgggcggcga gaatcggcgc gacgtcgacg tagctgtaga
     7681 tgtcgtacgg ccaaccgtgc aacaggatga cgggtgggcc gtcagctggg ccgtcctcgg
     7741 catagccgac gttgagcact gcggcgtcaa cctgtttgag cgacgcgaac gacgtgtgcg
     7801 actgcgccgg tgaagccgaa gcgattgcag aactggccac tgaggtagcg gcgaccgcga
     7861 atccgacggt gcgcgcgaag tcgcgtcgtg tgatcaccgt cagatcgttg agaattcgga
     7921 atcgacttgc aagtccagat cagctgaagg gtggccgcct gtcgatccgg cgcgacgcca
     7981 aacccgccga ccgccacacc cgcgcgatcc agtcgacgtc ctcgtcggtc accaaattcc
     8041 ccatcacccg caccgcgatg ttcatcagga acgccgatcg catcgcaaca ggacccgtcg
     8101 ccggcaggaa ccgcggaatc gtcagcagca gccccagccg gcgcgcgact gagaacgccc
     8161 gcgcgtaatg cgacgacagc acctcaggcc atgccgacga cacgtcgccg acgcccagca
     8221 tctcggccgc cagccggccg gtctccagcc cgtagtcgat gccctcgccg ttcagcgggt
     8281 tgacgcaggc cgcggcatcg ccgatcagca tccagttcgg cccggccacg ccggagaccg
     8341 cgccgcccat cggcaacaac gctgacaacg ccaaccgcgg ctcgccctcg aagccccatt
     8401 ccttacgcct cagtgacgtg tagtacgaca tcagcggccg caaagcggca tcggcggggc
     8461 gtttcgacgt cgccagcgcg ccgacgccga tattcacctc gccattgccg agcgggaaga
     8521 tccacccata tccgggcagc acccgcccct cgggtgagcg cagttccaag tgcgacgtaa
     8581 tccacggctc gtcggcgcga ggcgtcgcga tgtacccgcg gaccgccacg ccgtacaccg
     8641 tctgctgatg ccactgccgg cccaacaccc ggcccaacgg cgagcgtgcc ccgtcggcga
     8701 cgatcagcga cctgcacccg atctccgcgc cggagtccaa caacagcgac gacacgcgtc
     8761 cgctcgaatc atggcgcaca tcaacggctt tcacgcccag cagcatcttg gcgccgtcgt
     8821 cgacggcgac catccggatg cggtcgtcga gctctgtacg tggcaccgcg ctgctgaccg
     8881 ccgggaacga cgggcccggc cagtcgacct ctacgtcacc gccgaacccg acaagccgca
     8941 agccgcggtg ctgtacccgg ccctcgagcc acggcccgag ccccagccgc tgcaactcca
     9001 agacggcgcg cggggtcagg ccgtcgccgc aggccttgtc gcgcgggaac tgcgcggagt
     9061 cgatgaccag cacgtcgcgc ccgccccggc aggcccacgc ggccgcggcc gagcctgccg
     9121 gcccggcccc gacgacgacc acgtcggctc gtgtatccac gcctcccagt atgttggcag
     9181 ggtgaggaca ccagcgagtg tggtggcggg ggtggatttc gccgaccccg tcttcgcgca
     9241 ggaggtccgc gatggtgtgg cgcgcatcga ggacctgatg gccactgagc tgggcaaagc
     9301 cgacgagctg atgtccgagg ccgtccagca cctgttccag gcaggcggaa aacggttccg
     9361 cccgctgttc accgtccttt cggcgcggct ggggccagaa cccgatgcat ggcaggtctc
     9421 cgtcgcgggg gccgtcatcg agctggtgca cttggcaacg ctctaccacg acgacgtaat
     9481 ggacgaggcg caggtgcgcc gcggcgcccc cagcgccaac gcccgctgga gcaacaacat
     9541 tgcgatcctg gccggcgact atttgttcgc gacggcctcc cgcctcgtct cccgactggg
     9601 tccggaggcc gtgcgcgtca tcgccgatac cttcgcgcaa ctggtgacgg ggcagatgcg
     9661 tgagacccgc ggcgtggccg agggtgtcga cgaggtcgag cactacctga aggtggtgta
     9721 cgaaaagacg gcgtgcctga tcgcggcgtc gggccggttc ggcgcgacgt tctccggagc
     9781 cggcgaggag cagatcgagc ggctgagccg gctcggcggc atcgtcggca cggcgttcca
     9841 gatctccgac gacatcatcg acatcgacag cgaccccgac gaatcgggca aggtgccggg
     9901 caccgacctg cgcgagggcg tgcacacact gccggtgctg tacgcgctgc gtgagacggg
     9961 cccggacggg gaccggcttc ggcagctgct caaaggtccg gtcgaaaacg atgacgacct
    10021 ggccgaggcg ctgcggctgc tccgcaagtc gccgggaatg gcgcaggcca aggacaccgt
    10081 ggcgcggtac gccaaacaag cccgcgacga gctggccaac cttcctgaag gcccgggtcg
    10141 ccaagcgctg gccacgctgg tggactacac ggtgaacagg cacggctgac gcgacaggaa
    10201 cccgcggagg ctggctaagc gttgactcag cgaagacttc gtaggaggta gctgatgacc
    10261 tggcatccgc acgccaacag ggccaagacg ttcctgttgc tggtgggtat gtcggcgctg
    10321 atcatgttca tcggcgcgct gttcaatagc agggcgattc tgggcctggc ggtgctgttc
    10381 gccgtcggca tgaacgtcta tgtctacttc aacagcgaca agctggcgct gcgggcgatg
    10441 catgcccagc cggtgaacga gatgcaggcg ccggtgatgt accgcatcgt gcgggagctg
    10501 gcgacgacgg cacgacagcc aatgccgcgg ctgtacatca gcgataccgc ggcaccgaac
    10561 gcgttcgcca cgggccggaa cccgcgcaat gcggcggtgt gctgcacgac aggaatcctg
    10621 cagatcctca acgagcgcga gcttcgtgcg gtgctgggcc atgagctttc ccacgtgtac
    10681 aaccgcgaca ttctcatctc gtgtgtggcc ggtgcgatgg cttcagtgat cacggcgctg
    10741 gccttcgtcg cgcacttcgc gggaatgttc ggcgggagcc gcgacggtgg ctcgaatccc
    10801 ttcgcgctgt tgctgattgc gctgttgggt ccgatcgccg caacggtgat ccggctggcg
    10861 gtgtcgcggt cgcgtgagta ccaggctgac caatcgggcg cggagctgac gggcgatccg
    10921 ctggcgttgg cttcagcatt gcggaagatc agcgcaggtg tgcagcaggc gcctctgccg
    10981 ccggagcccc agctggcaga tcaggcgcac ctgatgatcg ccaacccgtt ccgctccggt
    11041 gagcgcatcg gcaagctgtt ctcgacgcac ccgccgatgg aggaccggat ccgcaggctg
    11101 gaagcgatgg caggccgcgg acctgggttg tactgaggtc ggcgtgcgcc ctttggccgc
    11161 gccccctagg gtgtggtccg tgctgcgcag aattctgatc ggcgttgcgg ggatcgcggg
    11221 ggcggccctg gttggcgtgc cgggcatcgc ggcggctgac ccgccaccgc tgccgaacat
    11281 caatgcgttt ccgtcggcga agccgtcgga gtactcggtg aacgacggcg cccggtacgc
    11341 cttcggcgtg ctcgacggcg ttacgtgcgt cctggacaag cagtcgggca gttatggctg
    11401 cagcgggccg attcccgctg cgccgggcgg cgcgaatctg gtcagtgcac gtgcgtcggg
    11461 taagcctgga ttcgccaacg ctgaccgccc cgtgtacggc gtggtcgagg atgcgaaggc
    11521 gctgccgccg aatacgcggc tcagcttccg cacggtcagc tgtgggacgg acgggttcgt
    11581 cacgacgtgc ctgaacagca tcgaccagtc gggttttgtg ctcagccccg acggcagcta
    11641 cacgttcggc tagaaacact gctggtcatt tctgcgacac ataggctatg gcagtgctga
    11701 gcagagttct ggcaggcgtt ttgggagcag tggccgtcct cggcgtgcct ggcattgcgg
    11761 gggctgagcc gccgccagat ccaccgccac agccgcctcc accacccaac gtcaacgcct
    11821 tggcgccggt gaagctgtcc gattacgcga ccatgaacga cacctggtac gcgttcacgt
    11881 cgggcggagt cacctgcgtg ctgcagcgcg cgggcagcta tgggtgcagt ggtccaattc
    11941 ccggcgcccc cgagggggcg aacctcgtca gcggctccta tggcggggtg ccgggcttcg
    12001 cgatctcacc cggcgacgtg ttcgccgcgg ccggtgcggt caaaccgctg ccggctggtt
    12061 cgcggatcag ctatcagacc gtcagttgcg gcaacgacgg cgcgacaacg acgtgcgtcg
    12121 acggtcgcag ccagtcggga tttgtgatca gcccggcggg cagcttcatt ctcaacgaga
    12181 cacctccgct gctgtaccga cccgataaca ggggtccgtt cgctaactga gctttcgtgc
    12241 cgcgaaaccg ggtactcgaa gggaacgaga ccggggaggc acgaatggcg aaggatcatg
    12301 gcccaagcgt caagaacgac aagcagtacg agggcctgcg caagaagggc atgagcaagg
    12361 cgcgggccgc gaagatcgcc aatacgcctg gagcttcaaa gaagggcggc aaaaagagcg
    12421 ggaagaagtc cagctagttc ggtctgcggg ccgccatcat ttgttgacgg ccgccaaccg
    12481 gctagtgctc ctcagaaccc cgagccgtgc acctcgtggc ccggcttctc cgcgacgagc
    12541 ccgcggtacg cgtcttcgac cgtgctgccg tggttgatga cgccgtcgac ctcgcgggcg
    12601 atcggcatgt tgatgccgta tttgtcggcg aattccatga tgacgctgga ggccttgacg
    12661 ccctcggcga cctgattcat cgacgcgatg atctcgtcga tcggcttacc ggcgcccagc
    12721 tgttcgccga cgtgacggtt gcggctgcgc tggctggtgc acgtcacgat cagatcgccg
    12781 gtcccggcca ggcctgcgaa cgtgtcgcgg tgcccaccga ccgcctcgcc tagcttcgac
    12841 atttcgcgca ccgcgcgcgt gatcaccatc gcgcgcgtgt tttcgccgat gcccagcgag
    12901 tagcccatgc cgaccgcgat cgcgtagacg ttcttcagcg cgcctgccat ctcaacaccg
    12961 acgacgtcgt cggtcgtata ggtgcggaat cgcttggtgc ggaacagttc cgccaatttg
    13021 gcggccaggt gctgatccgg catcgccagc accgcggcag cggcgtatcc ctctccgacc
    13081 tcgcgcgcga tgttcggccc ggccagtatg cccgccggat ggccgggcag cacctcatcg
    13141 acgatctggc tcatccggta gttcgtgccc tgctcaaggc ctttgaccag ggacaccacc
    13201 ggcacccacg gccgcagctc cttggccagc tgttcgagca cgccacggaa gccgtgcgac
    13261 ggcacgccca tcacgacgac gtcggcggca ttggccgctt cggagaagtc gttggtggct
    13321 tgcagcgttg gcgacagctc gacgtcatcg ccgagatact tcgtgttgcg gtgattcttg
    13381 ttgatgtcct cggcggtctc ctccgagcgc acccactgca acgttggccc gcgcctggcg
    13441 cagatggacg ccacagtggt gccccaggat cctccgccga ggacgacgac attgggttcg
    13501 cgttgcgcag gtgccatgcc gatcagcgta ggtctgtcgg ctggcaggcc ggggcggttt
    13561 agcagtgcga atccgactga cttgtgcgct gctgccgtgg tggacgggta cgttcaaagc
    13621 gtccgcagcg cgcataggcg tacccatgtc agacccgact cgcgtcgatc ctcaaaagcc
    13681 ggcggccgcc gtgaccccaa ccggtggcag cgccgaagag gtcaatgcgt ggtggggttc
    13741 gctcggccag gcggaccgag accgactgct cgccgagcac ccgtcggggc tggggaatct
    13801 caatggcatc ccggccggca tccgagacca gatcaataac gcggtgatga acgacgacct
    13861 atcccgcatc gcggacgcgg ccgccgggca cgccgtatcg gtcgacgatg tgttggcaag
    13921 tcctgcgcgc tatggccttt cgaacgatga cgccgtgcgg tatcggaacg ccgtccaaac
    13981 caggcggggc ctcgaccacg accggggatc cgacccgggc aacccgcgac cggtgatgct
    14041 gtgggcttat cacccgtcgg catttcacgg gcagggcagt gcagcgatcg cgatcggtga
    14101 cccggacgaa gcgggcgaca tcgcggtcat cgtgccggga gcgggtagca gcgtcgcggg
    14161 cggatggttg tccggagggc acaacgctgc catcaacgtc tatgaccggt cgattgccgc
    14221 gtatccagac gaagacatgt cggtgatcgc gtggatgggt tacgacgcgc cagacaattt
    14281 cagcgatctg ggaatcgcgg cgccgttgtt ggcccgagcc gggggtgagc tgttggccca
    14341 ggacgtcaac ggcctgtggg tgacgcacgc ggaatcgaac ccaccccacg tgagcgtgct
    14401 cgggcattcc tatggggcga cgacggtcgc ggatgccttc gccggcagcg gcatgcgtgc
    14461 gaacgatgcg atcctgctgg gcagcccggg gacagaccta gccaagagtg ccgcagactt
    14521 ccacgtcgac tccggcgagg tgtatgtcgg cgccgcgtcg acggaccccg tcagctggat
    14581 cggagtatcg ggaagcgtgc cggacatact gaacgacgtg tttggccatc cattcggccc
    14641 cgacgttggg ctgggcgccg acccggccgg tgatggtttt ggctctgttc gcttcgaggc
    14701 ggaggtgatg gggcgcgaca ggctggacat gggtgaccat tcgcattact acgacgtcgg
    14761 cggcgaagcg ctgcgcagca tcgtccacat cgtcaccggc aatggcggtg cgctggcacg
    14821 ggacgacttg gtggcggagg gaaggcggca gccgcatgcg accacgcctc gcgagatcaa
    14881 tattcccggg ctgggccgaa tcagcttgcc acacattgac actcgcatac cgggcacgcc
    14941 cgcctcgatc gacccggaag ccgttcgcgg attggagtca acgtgactga ggacgcactg
    15001 cctcggctgt ctgacgaaga ggcaatggcg caagtggtcg agccggccag gcagatcatc
    15061 cggcttgcgg gcctgcagga cgtgagcggg ggcttcttat tcgagtcgtg caacgaccag
    15121 ggtgacccgc cgtatcgcgg gcgcgtagag atgagcttcg cgatgcccgc cggcgccgag
    15181 cccgatgcgt actttcagca gattgcagcg gtgatgctga cgcaggggtg gatcgacgga
    15241 cctccgccgg ggaagtgtcc gatcggtgtg gtcattcaca cagagacggt catggcgatc
    15301 atcgccaggg catcagcggc cgacatgcgg gggtccgtcc agctgtgcgg gcagtgcggc
    15361 aacatcacgg accaccgcgc cgacggcaag accgtcgggg tcgagatcac ggcgcagctc
    15421 accggtagtt gacgaactgc agcgccacgt cgaggtcggc cttcttgagc atggcgatga
    15481 cggcctgaag gtcgtcgcgc ttcttggagc tgacgcggac ctcgtcgccc tggatctgag
    15541 tcttgacgcc cttggggccg tcatcgcgaa tcagcttggt gatcttcttg gcgtcctcgc
    15601 tgctgatgcc ctgcttgatg gcgccgctga ccttaaaggt cttgccgctg gcctggggat
    15661 cgccgacgtc gaaggccttc atggagatgt cgcggcggat gagcttttcc ttgaacacgt
    15721 cgatggccgc cttgatgcgc tcctcggtgg acgaggtgat cacgacggtc tcctcgccct
    15781 gccacgcgat cgacgtgtcg gtgccgcgga agtcgaagcg ggtggccagc tccttggcgg
    15841 cctggttcag cgcgttgtcc acctcctggc ggtcaacctt gcttacgaca tcgaacgatg
    15901 aatccgccat tggattcggc cctcctcggt gcgtctactt gtgaactcgg ggacattctc
    15961 gtcctagcgc ttcgggcgca ccaaggcagg tgcacggcag tcgat
//
LOCUS       DASRJA010000337         9793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_515791,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000337 DASRJA010000000
VERSION     DASRJA010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9793
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515791"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFC01_14885"
     CDS             <1..184
                     /locus_tag="VFC01_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HZN80952.1"
                     /translation="AHRALADIHESIRELKYYRHTAFVPPPGPSTSEIAEIAAELGPS
                     NGASEVIDSAAERPSG"
     gene            213..285
                     /locus_tag="VFC01_14890"
     tRNA            213..285
                     /locus_tag="VFC01_14890"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:246..248,aa:His,seq:gtg)
     gene            complement(304..1524)
                     /locus_tag="VFC01_14895"
     CDS             complement(304..1524)
                     /locus_tag="VFC01_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZN80953.1"
                     /translation="MWQVKKVARPRTRAWLVAVALIPAVVLGLSACAKETAPPKPQVI
                     TDKGTPYGDLLVPKLTASVKDGAVGVSVESPVTVSAEDGVLGAVTMVNADDGTPVAGQ
                     LSPDGLTWSTAEPLGYNESYTLNAQSLGLGGATSQQMTFETHSPENLTMAYVLPNEGE
                     VVGIGQPVAVQFDENIPNRLAAQKAIKVTTNPPVEGAFYWLNNREVRWRPAQYWKPGT
                     TINVQVNTYGVDLGDGLFGQENVSTQFTIGDEVIATADDNTKIVTVRRNGEIVKTMPT
                     SMGKNSTPTNNGVYIVGSRFSHIVMDSSTYGVPVNSPNGYRTDVDWATQISYSGIFVH
                     SAPWSVGSQGYSNTSHGCLNVSPSNAVWFYDNTKRGDIVEIINTVGSTLPGTDGLGDW
                     NIPWEQWKAGNANV"
     gene            1684..1756
                     /locus_tag="VFC01_14900"
     tRNA            1684..1756
                     /locus_tag="VFC01_14900"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1717..1719,aa:Lys,seq:ctt)
     gene            complement(1758..2024)
                     /locus_tag="VFC01_14905"
     CDS             complement(1758..2024)
                     /locus_tag="VFC01_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80954.1"
                     /translation="MIDDADLAQARELLRSLHAHVDEISKKVESAESRTRRMQGHAAL
                     VARRRVASLRHELYEAHRLIDGLHTRFPDARLRRVAGPRTECSR"
     gene            complement(2568..2795)
                     /locus_tag="VFC01_14910"
     CDS             complement(2568..2795)
                     /locus_tag="VFC01_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HZN80955.1"
                     /translation="MADRDPDTIKRDIDQARDQLAVTVDNLAERANPQRIADDIKSGV
                     VRFVKKPAVAVSLAGVGALVVVLVVRRIRRR"
     gene            2896..3375
                     /gene="bcp"
                     /locus_tag="VFC01_14915"
     CDS             2896..3375
                     /gene="bcp"
                     /locus_tag="VFC01_14915"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HZN80956.1"
                     /translation="MPQTPRLEVGDKAPAFSLPDADGNTVKLSDYKGRRVIVYFYPAA
                     ATPGCTKQACDFRDSLSELNGAGLDVIGISPDKPAKLAKFRDDQKLTFPLLSDPDRKV
                     LTAWGAFGEKTMYGKTVQGVIRSTFVIDEKGKIAEAQYNVRAAHTTTKIQKIIAGSR"
     gene            4089..4466
                     /locus_tag="VFC01_14920"
     CDS             4089..4466
                     /locus_tag="VFC01_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN80957.1"
                     /translation="MTATERITELLYRNLQEVFGEGDSARRHAAIEEFYTDDCALYVP
                     DGVLVGHDALDKFAGELRATHPNFVYTPHGEPQALHNAGILAWGSGPKGEPPEYTGLD
                     VVVVRESKIGALYVFLNPKLSSA"
     gene            4443..4523
                     /locus_tag="VFC01_14925"
                     /pseudo
     CDS             4443..4523
                     /locus_tag="VFC01_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009474120.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
     gene            complement(4500..5846)
                     /locus_tag="VFC01_14930"
     CDS             complement(4500..5846)
                     /locus_tag="VFC01_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZN80958.1"
                     /translation="MSDLVERVREVLPSVRRDLEDLVRIESVWADPVRRPEVQRSAEA
                     VAKLLSDAGFGDVQIVSDGGAPAVIARHPAPPGAPTVLLYAHHDVQPEGDHGQWASPP
                     FEPTERDGRLYGRGTADDKAGIATHLAAFRAHGGAPPVGVTVFVEGEEESGSPSLSRL
                     LAAHRDVLASDVIVIADSDNWSTDVPSLTVSLRGLADCIVEVATLDHGLHSGLWGGVA
                     PDAVSVLVRLLASLHDDDGNVAVAGLHEGTAADVDYSPGRVREEAGLLDGVSEIGSGS
                     VPQRLWAKPAITVIGIDVTSIDKSSNTLIPRARAKVSMRVAPGGDPAKHLAALTRHLE
                     EHAPWGARVIVTPGDMGQPYAIDASGPVYDATRAAFRQAWGNQPVDTGVGGSIPFIAE
                     FAAAFPAAKILVTGVEDPQTQAHSINESLHLGVLERAATAEALLLQNLGSNGQVAP"
     gene            complement(5843..7267)
                     /locus_tag="VFC01_14935"
     CDS             complement(5843..7267)
                     /locus_tag="VFC01_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN80959.1"
                     /translation="MKRPPVARTTEGDDADSLSRSQTAFMVAALVTSVISFQLNATML
                     GPAIRDINTELGPDAFAAMSNYFYLAGAIANVIFIRWSDFVGRKRVLVGIMIAMSVGT
                     LLCIVGSSLPVVVAGRILQGGCNVSFGLAFLILRERLSGPAFGLCSGVIASINGGVGG
                     VDALAGGFMVDNFGYRSIFVLILVVGVAAIVLAVKAVPADASDRVASGRMDWVGAALI
                     ALTVAGINLFLGQGGHSGWSSPVALGFIGAAVVALGAFVVVEKRVATPLVDIDEMRSR
                     YAWPLIVVIVLCLASFTMVIGFIMPSIAENDDVGFGLSAEMTALLFLTPAAVAQLISA
                     PLIGRLAVRIGFVTVLRAGLIGTVPVGALLAIFAFNQNAAVLLLPVLGLAFGGVAGTA
                     LAVLGVTQARQDEPGSLPGLSNAAFGVGSSLGFAWAGPVVGQGTVAGFHSALWISVAI
                     GIVALGMSLVLKPRSTKTELASTP"
     gene            complement(7354..7746)
                     /locus_tag="VFC01_14940"
     CDS             complement(7354..7746)
                     /locus_tag="VFC01_14940"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZN80960.1"
                     /translation="MAVVGVGIDLVSIPDFAEQVDQPGTVFAETFTPGERRDAADKSS
                     SAARHLAARWAAKEAVIKAWSGSRFSKRPVLPEGIHRDIEVITDMWGRPKVRLTGAIA
                     EHLKDVTIHLSLTHEADTAAAVAILEER"
     gene            complement(7765..>9793)
                     /locus_tag="VFC01_14945"
     CDS             complement(7765..>9793)
                     /locus_tag="VFC01_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZN80961.1"
                     /translation="EMTAAEATEDEAEEHGTIRALPSPPRGYKPAPAPAWDDLDVDPA
                     DLVVIVGGAELGPYGSSRTRFEMEVENELSAAGVLELAWTTGLVKWEDDPTPGWYDSE
                     TGDLVDEGELVERYHDAVVERVGIREFVDDGAIGPDHAAPLLVSVFLDKDFTFVVSSE
                     EEARQFVQFDPEHTVIAPVPDSGDWQVTRKAGTEIRVPRKTKLSRTVGAQIPTGFNPM
                     VYGVNQEMMNSIDRLAIWNLVTTVDAFLSAGFTPAELMRWVHPSLVASTQGTGMGGMT
                     SMQTMYHGNLLGRNKPNDILQEVLPNVMAGHVVQSYVGSYGSMIHPVGACATAAISVE
                     EGVDKIRLGKAEFVVAGGYDDLTLEAIIGFGDMAATADTGMMRAKGISDSKFSRANDR
                     RRLGFVEGQGGGTILLARGDLALKMGLPVQAVLAYVSSFGDGVHTSIPAPGLGALAAG
                     RGGRESQLARSLAKLGVGPDDIAVVSKHDTSTLANDPNETELHERLADSLGRSAGAPL
                     FVVSQKSLTGHSKGGAAAFQMMGLCQILRDGVIPPNRSLDCVDDELASAAHLVWLRDT
                     LRLGEKFPLKAGLITSLGFGHVSGLIALVHPQAFIAALDPEQRQDYQARFEARVLAGQ
                     HRLASAIAGGPSLYERPADRRFSHEAPEKRQEAEMLLDPASRLGDGDVYVR"
BASE COUNT         1827 a         3279 c         3064 g         1623 t
ORIGIN      
        1 cgcccaccgc gcgctagccg acatccacga gtccatccgc gagctcaagt actaccgcca
       61 caccgcgttc gtgccgccgc ctggcccgtc gaccagcgaa atcgcggaga tcgccgccga
      121 actcgggccc tcgaacggcg cctctgaggt tatcgattcg gccgcggagc gccctagcgg
      181 ctaatatcga cgacgccgca tgtgcggcga tggtggctgt agttcagttg gtagagcacc
      241 aggttgtgat cctggctgtc gcgggttcga gtcccgtcag ccaccccgtt gaagcgggca
      301 acgtcagacg ttggcgttgc ccgctttcca ttgctcccac gggatgttcc agtcacccag
      361 cccatcggtt ccgggcaacg tcgatccgac ggtgtttatg atctcgacga tgtcgccacg
      421 tttggtgttg tcgtagaacc agacagcatt gctcgggctg acgttgaggc aaccatggct
      481 ggtgttcgaa taaccctggc tgccgactga ccacggcgcg gagtgcacga agatgccgct
      541 gtagctgatc tgcgtcgccc agtccacatc ggtgcgatac ccgttgggcg agttgaccgg
      601 cacgccatag gtcgacgagt ccatgacgat gtggctgaac cggctaccga cgatgtagac
      661 accgttgttg gtaggcgtgc tgttcttgcc catcgaggtc ggcatcgtct tgacgatctc
      721 accgttgcgg cggacggtga cgatcttggt gttgtcgtcg gcggtcgcga tcacctcgtc
      781 gccgatggtg aattgcgtgg agacgttctc ctggccgaac agcccgtcac cgaggtcgac
      841 gccgtaggtg ttcacctgga cattgatcgt cgtgcccggc ttccagtatt gcgctgggcg
      901 ccaacgcact tcgcggttgt tcagccagta gaaggcgccc tcgacgggcg ggttggtggt
      961 gaccttgatg gccttctgcg ccgccaaccg attcgggatg ttctcgtcga attggacggc
     1021 aaccggctgg ccgatgccga cgacctcgcc ttcgttgggc aagacatagg ccatcgtcag
     1081 gttttccggc gaatgcgtct cgaaggtcat ctgctggctg gtggcgccac cgaggccgag
     1141 cgactgcgca ttcaaggtgt agctctcgtt gtagcccagc ggctcggccg tactccaggt
     1201 caggccgtcg gggctgagct ggccggcgac gggagtgccg tcgtcggcgt tgaccatggt
     1261 cactgcgccc agcacgccat cctcggcgct gaccgtcacg ggggattcga cgctgacacc
     1321 gacagcgccg tccttgacgg acgcggtgag tttcggcacc agcaggtcgc cgtagggcgt
     1381 gcccttatcg gtgatcacct gaggcttcgg tggggcggtt tccttggcgc acgcggacag
     1441 cccgagcacg acggcaggga tcagcgccac ggctaccaac caggcacggg tacgcggacg
     1501 ggccaccttc ttgacctgcc acattctttg ctccacttca caaccaaaac tcacggacag
     1561 acgattcttg gcaatagtct aagggcaaac ttagctaggg gcgcgaccgc ggcggcgctg
     1621 ccagcggcag cgagcggtcg aatttcaccc tgcgccgcaa ggcctgttat tgtcgcacac
     1681 gcagcgccgt tagctcagtt ggtagagcag ctgactctta atcagcgggt ccggggttcg
     1741 aaaccctgac ggcgcactca gcgtgaacac tcggtgcgcg gcccggctac tcgccggagt
     1801 cgggcgtccg gaaagcgtgt atgtagcccg tcgatcagcc ggtgcgcttc gtagagttcg
     1861 tgacgcaggc tggcgacccg gcgccgggca accagcgccg cgtggccctg catccgccgc
     1921 gtgcggctct cggcagattc caccttctta gagatttcat cgacatgggc atgcagtgat
     1981 cgcagtagct ccctcgcctg cgctagatcc gcgtcatcga tcacgtcggc atcatcgcat
     2041 caaccgggca caaccgcgcg ccgtggtcga tcgggcaaac cgcccaatag ccgtcggcat
     2101 gacactgccg caacgaagcc cagcggtact tgcccaggca actgctgcgc agtagcctat
     2161 tgacgcgagc gaagaaaaag cgtcgcgtct gctgatcagc gatctggaat gtgctactag
     2221 tcacataagt cgacttatac tacgaacccg tcaagtgtgt taccagtaac ggaagggatc
     2281 cgttcacagc gatttatgcg gcatagaggg tgtaagggtg ctagtctctg gttagctaca
     2341 acgaattaca tggccacgcg gactacggaa tgaggtctta gctgatgtcc acgatagacg
     2401 catcactcac cgcgggtgcg tccggcgcgg ggtcagctga cgacgtaact gtgcaacggc
     2461 ccgtcatggt ttacagctgc aatcgcctcg gccgagtcgc caacccgccc agcttctcgt
     2521 tcgccgattg gttccgctcc gcgtgtcgcc gctgagaacc caatccgtca gcgtcgccgg
     2581 atgcggcgca ccacaagcac gacaacgagg gcgccgacgc ctgccagcga cacggcgacg
     2641 gcaggcttct tcacaaaccg caccacgccc gacttgatgt cgtcggcgat ccgctgcgga
     2701 ttggcccgct cggcgaggtt gtcaacggta accgccaact gatcacgagc ctgatcgatg
     2761 tcccgcttga tggtgtcggg atcgcggtcc gccactttgt ccctccaagc ccctcaatgt
     2821 gcgctgcctt gccgaactac cctaattgag cttgcgctca ccggagtgca ccggtcgaag
     2881 aagggacctt tccgcatgcc gcagaccccg cgtctcgagg tgggcgacaa agcccccgcg
     2941 ttcagcctgc ccgatgccga cggcaacacc gtcaagctct cggactacaa gggccgcagg
     3001 gtcatcgtgt acttctaccc cgccgccgcc acacccggat gcaccaagca ggcatgcgac
     3061 ttcagggaca gcctcagcga actcaacggc gccgggctgg atgtcatcgg catctcccct
     3121 gacaaaccgg ccaagctggc caagttccgc gacgaccaga agctgacgtt tccgctgctg
     3181 tccgatccgg accgcaaggt gctcacggca tggggtgcct tcggcgaaaa gacgatgtac
     3241 ggcaagaccg ttcagggcgt gatccgttcg acgttcgtca tcgacgagaa gggcaagatc
     3301 gccgaggcgc agtacaacgt gagggctgca catacgacaa caaagatcca gaagatcatc
     3361 gctggtagca ggtaattcct gtgtcgttct gggacatggc cacagaagcc atggaggctg
     3421 cggtcgtcaa ggtcgtccac tacttcgacg gtgccggtga cacagaggca gtagaaaact
     3481 ccgcattgag ttcgaagccg gaacctcact cccccctctc ggcatcctta aggacatggg
     3541 ctaccgcgtc ggaaagaacg gactattacc ccaccagggc gtgagattct ccgacgaacc
     3601 cttagggtcc aactcgtcgc taccaagccg tggacaaagg agtacatccg cgagtggggt
     3661 gggcgatgca gccaggccag gttggacaag acggaccggg ttctgggcgg gttggtggca
     3721 aagcgcgaaa cgaaagacga gagttgacat gtcggaggcc atccgcgact ggaaagaaga
     3781 ccaggcgtgg ttgagggaca gcaaggtgga gtggctgacg cacacccacg agcggtaact
     3841 caaaccaggg aagatcgtca atccgctaca aggcgcctcg ggtccgggag gcgcagtcca
     3901 acgtcaaggc tgcacacacg acaacattta tcaagaaagc gctcagcgcc tcgtaatcag
     3961 tcgatcgagc cgccgcgccg gctgccgagc tgattcgacg cggaagcgga atgaaatagg
     4021 accgcagtcc gtttagcatc tccgaaggcg tcggacaggc tgacacctcg acgaaggacg
     4081 acgcgaatat gactgcgact gagcgaatta ctgaacttct ctatcgaaac cttcaggagg
     4141 tcttcgggga gggcgattca gcccgccggc acgccgcgat cgaagagttc tacacagacg
     4201 actgcgcgct ctacgtcccg gacggcgtcc tcgttggaca cgacgcacta gacaaattcg
     4261 ctggggaact acgtgcgact caccccaatt ttgtctacac accccatgga gagccgcagg
     4321 ctctacacaa cgcgggaata ctggcatggg gctcgggccc gaaaggtgag ccacctgagt
     4381 acaccgggct cgacgttgtc gttgttcgcg agagcaagat cggtgcactg tacgtcttcc
     4441 tcaatccgaa gttgtcatcc gcctagtaca acgtccgcgc caccggccac gtcgcgaagc
     4501 tacggcgcga cctgtccgtt tgacccgagg ttctgcagga ggagcgcctc cgccgtcgcc
     4561 gcgcgttcca gcacgccgag gtgcaggctt tcgttgatgc tgtgcgcctg cgtctgcggg
     4621 tcttccacac cggtgaccaa gatcttcgcc gccgggaacg ccgccgcgaa ctcggcgatg
     4681 aacgggatcg aaccgccgac gccggtgtcg acgggctggt ttccccaggc ctggcgaaat
     4741 gcggcgcggg tggcgtcgta gaccggtccg ctcgcgtcga tggcgtacgg ctggcccatg
     4801 tcgccgggcg tgacgatcac ccgcgcgccc catggcgcgt gctcttccag gtgacgggtg
     4861 agcgcggcca gatgcttagc cgggtcgccg cccggcgcca cgcgcatgct caccttcgcc
     4921 cgcgcccgcg ggatcaacgt attcgacgac ttgtcgatcg aagtaacgtc gatgccgatc
     4981 accgtgatcg ctggcttagc ccaaagtcgt tgcggcacag agcctgagcc gatctccgac
     5041 acgccgtcga gcaagccggc ctcctcacgc acccgccccg gcgagtagtc gacgtcggcg
     5101 gccgtcccct cgtgaagccc ggcgaccgcg acgttgccgt cgtcgtcgtg caggctggcg
     5161 agcagtcgga ccaggacact caccgcatcg ggtgcgacac cgccccataa tccggagtgc
     5221 agtccgtgat cgagcgtcgc cacctccacg atgcagtccg caagcccgcg cagcgacacc
     5281 gtcaatgaag gcacgtcggt gctccagttg tcggagtcgg cgatgacgat cacgtcggac
     5341 gccagcacgt cgcggtgcgc tgccagtagc cgcgacagcg acggcgagcc cgactcctcc
     5401 tccccctcga cgaacaccgt cacaccgacg ggtggcgcgc cgccatgggc gcgaaacgcc
     5461 gccaaatgcg ttgcgatgcc tgccttgtcg tccgcagtac cgcgaccgta cagtcggccg
     5521 tcgcgctcgg tcggctcgaa cggcggcgag gcccactgac cgtgatcgcc ttccggctgc
     5581 acgtcgtgat gggcgtacaa aagcaccgtc ggcgctcccg gcggggcggg gtgccgggcg
     5641 atgacagcag gcgcgccacc gtcgctgacg atctgcacat cgccgaaccc cgcgtcggac
     5701 aacagttttg ccaccgcttc ggcgctgcgc tgcacctcag ggcggcggac cgggtcggcc
     5761 catacggatt cgatgcgcac caggtcttcc aggtcacgcc gcaccgacgg caggacctca
     5821 cgcacgcgct ccacgagatc gctcatggag tcgaggctag ctcagtcttc gtcgacctag
     5881 gcttgagaac caggctcatc ccaagggcga cgatgccgat tgcgacgctg atccacagcg
     5941 ccgaatgaaa cccggccacc gtgccctgcc cgacaaccgg gccggcccag gcgaatccga
     6001 gcgatgatcc gacgccgaag gcggcgttgg acagaccagg cagcgacccc ggctcgtcct
     6061 gccgtgcctg cgtgacgcca agaaccgcga gcgcggtacc cgccacaccc ccgaatgcca
     6121 atccgaggac cggtaggagc aggacggccg cgttctggtt gaacgcaaag atcgcgagga
     6181 gagcgccgac gggcacggtg ccgatcagac ccgccctcaa caccgtcacg aagccaatcc
     6241 ttaccgccag ccggccgatc agcggtgctg aaatcaattg cgccactgcg gcgggagtca
     6301 ggaacaacag cgccgtcatc tccgcgctga gtccgaagcc gacgtcgtcg ttttcggcga
     6361 tggacggcat gatgaagccg atcaccatcg tgaacgacgc caggcacagg acgatcacga
     6421 cgatcaacgg ccaggcgtag cgcgagcgca tctcatcgat gtccaccagt ggtgtcgcca
     6481 cgcgcttttc gaccacgacg aacgccccca acgcgacgac ggccgcgccg atgaacccca
     6541 gtgcgactgg cgaagaccag ccggagtgcc caccctggcc gaggaacaaa ttgataccgg
     6601 cgacggtcag cgcgatcagg gcggctccca cccagtccat ccggcccgag gccacccggt
     6661 ccgaagcgtc ggcaggcacc gccttgacgg caagcacgat cgcggccacg ccaaccacca
     6721 ggatcaagac gaagatcgag cgatatccga agttgtcgac catgaagccg cccgcgagcg
     6781 cgtcgacccc gcccaccccg ccgttgatcg acgcgatgac accgctgcac aagccgaagg
     6841 ccgggccgct gagccgttcc cgcaggatca agaacgccag accgaatgag acgttgcaac
     6901 caccctgcag gatccggccc gccacgacta ccggcaatga tgagcccacg atgcacaaca
     6961 gcgtcccgac ggacatcgcg atcatgatgc cgaccagcac gcgcttgcgg ccgacgaagt
     7021 cgctccaccg gatgaatatg acgttggcga tcgccccagc gagatagaag tagttcgaca
     7081 tcgccgcgaa cgcgtcgggt ccgagttcgg tgttgatgtc gcggatcgcc gggcccaaca
     7141 tcgtcgcgtt gagctggaat gagatcaccg acgtgaccag cgccgcgacc atgaacgcgg
     7201 tctggctccg cgacaacgaa tcagcgtcgt cgccttccgt ggtccgcgct accggaggcc
     7261 gcttcatgac gcttgcattc cagcgcgagc agacgtaaaa gtgcccaaaa acgggcaaaa
     7321 aagggcaagt ttgcgtctgc tcgccggaga aacttagcgc tcctcgagaa tggcgacggc
     7381 cgccgcggtg tcggcctcat gggtcagcga caggtggatc gtcacgtctt tcaggtgctc
     7441 cgcgatcgct ccggtcagcc gcaccttggg ccgtccccac atgtcggtga tgacctcgat
     7501 gtcgcggtga atgccttccg gcagcaccgg cctcttggag aaccgcgaac ccgaccacgc
     7561 cttgatgacc gcctccttgg cggcccaccg cgcggccaga tgccgtgccg cagacgaact
     7621 cttgtccgcc gcatcgcggc gctcaccggg agtgaacgtc tcggcgaaca ccgtgcccgg
     7681 ctggtcgacc tgttcggcga agtcgggaat ggagaccaga tcgatcccca ccccgactac
     7741 tgccatggga ggaacgctag cgcactagcg cacatacacg tcgccgtcgc cgagacgcga
     7801 cgccgggtcg agcagcatct cggcctcctg ccgcttctcc ggtgcctcgt ggctgaaccg
     7861 ccgatcggcg ggccgctcgt acagcgacgg gccgccggcg atcgccgacg cgaggcggtg
     7921 ctgaccggcc agcacccgtg cttcgaagcg cgcctgatag tcctgccgct gttcgggatc
     7981 gagcgcggcg atgaacgcct gcgggtgcac cagcgcgatc agacccgaca cgtgaccgaa
     8041 cccgaggctg gtgatcagcc ccgccttcag cgggaacttc tcccccagcc gcagggtgtc
     8101 acgcagccag accagatgcg cggcactcgc gagctcgtcg tcgacgcagt ccaggctgcg
     8161 gttgggcggg atgacgccgt cgcgcaggat ctggcacagc cccatcatct ggaacgcggc
     8221 cgcaccaccc ttcgagtgac cggtcaggct cttctgcgac acgacgaaca gcggtgcacc
     8281 ggcggaccgg ccgagcgagt cggcgagccg ttcgtgcagc tccgtctcgt tcgggtcgtt
     8341 ggccagcgtc gaggtgtcgt gtttggacac caccgcgatg tcgtcggggc cgacgccaag
     8401 cttcgcgagc gagcgggcca gctgcgactc gcgaccgccg cgaccagcgg ccaacgcgcc
     8461 gaggcccggt gccggaatcg acgtgtgcac accgtcgccg aacgacgaga cgtatgccag
     8521 gaccgcctgc accggcagac ccatcttgag cgcgaggtca ccacgggcca gcaggatggt
     8581 gccgccaccc tgaccctcga cgaacccgag ccggcgacgg tcgttggcgc gtgagaactt
     8641 cgaatcgctg atgcccttcg cacgcatcat cccggtgtcc gcggtcgcgg ccatgtcacc
     8701 gaagccgatg atggcctcca gcgtcaggtc gtcgtaaccg ccggccacca cgaactcggc
     8761 cttgcccaac cggatcttgt cgacgccctc ctcgaccgag atcgccgcgg tggcgcaggc
     8821 gccaaccggg tggatcatcg agccgtagct gccgacatac gactgaacca cgtgtcctgc
     8881 catgacattc ggcaggactt cctgcaggat gtcgttcggc ttgttccggc cgagcaggtt
     8941 gccgtggtac atggtctgca tcgaggtcat gccgcccatg ccggtgccct gcgtgctggc
     9001 aaccaggctc ggatgtaccc agcgcatcag ctccgcggga gtgaagcctg cggacaggaa
     9061 cgcgtcgact gtggtgacca gattccagat cgccaaccgg tcgatcgagt tcatcatctc
     9121 ctggttgacg ccgtacacca tcgggttgaa cccggtgggg atctgcgcgc cgaccgtgcg
     9181 ggacaacttc gtcttgcgcg gtacccgaat ctcggtgccc gccttgcggg tgacctgcca
     9241 gtcgccgctg tccggcaccg gcgcgatgac ggtgtgctcc ggatcgaact gaacgaactg
     9301 gcgggcctcc tcttcggagg acaccacgaa cgtgaagtcc ttgtcgagga acaccgaaac
     9361 cagcagcggc gcagcgtgat cggggccaat cgcgccgtcg tcgacgaact cgcggatgcc
     9421 gacgcgctcg acgaccgcgt cgtggtagcg ctccaccagc tcgccctcgt cgaccaggtc
     9481 gccggtctcc gagtcgtacc agcccggcgt cgggtcgtct tcccacttga ccaggccggt
     9541 ggtccacgcc agctcgagca caccggccgc cgacagctcg ttctccactt ccatctcgaa
     9601 gcgggtgcgc gacgagccgt acgggccgag ttcggcacca ccgacgatca ccaccagatc
     9661 ggccgggtcg acatcgaggt cgtcccatgc aggagcgggc gcgggcttgt aaccgcgcgg
     9721 cggcgacggc agcgcacgga tggttccgtg ctcttccgcc tcgtcctcgg ttgcctcggc
     9781 cgccgtcatc tcc
//
LOCUS       DASRJA010000338         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2027776,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000338 DASRJA010000000
VERSION     DASRJA010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027776"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFC01_14950"
     CDS             <1..333
                     /locus_tag="VFC01_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN80962.1"
                     /translation="PAPLRKIPILIGGSGPKRTLPAVARHADIWHTFLPIDSYREASA
                     RVAELAAGFGRADSDIERSTLWTDPQSADAYLAAGATLFHTEVRASEGKYDLSTVEKL
                     VDWRDTKR"
     gene            complement(371..1177)
                     /locus_tag="VFC01_14955"
     CDS             complement(371..1177)
                     /locus_tag="VFC01_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZN80963.1"
                     /translation="MPTTQEKTWRAGRFWWDATAPTQSVVYPLRAVIFDLDALTDIEC
                     DGHRVAFNAAFAAHGLDFQWSVGRYRQLLALSDERQRVCAELRKRGIATECDVLTELL
                     ADEIYKTKTMMFEEMVRDADLAPRPGLVDLVMDAFAAGIWVGVVTNGPRSWSEPLIRH
                     LVGDGLVETVVTADDVKKPMPDREAHQNALWELGIPAENALAITGSAAGLRSATSAGL
                     ATVVVTGEGTPDLPAAMAVRADYSSGEPLGIVECQRLHGSWWAAHKRAAA"
     gene            1282..1947
                     /locus_tag="VFC01_14960"
     CDS             1282..1947
                     /locus_tag="VFC01_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN80964.1"
                     /translation="MTSGSPPVRLVLVDDHEMVIEGLKAMLAAFEDRVLVVGQAVGAE
                     RALSVVDDLDPDIVLCDVRMQGASGLDLCLQLRESDPKRKVVMLSVYDDEQYLFQALR
                     VGASGYLLKSISSDELVRQLEFVHKGETAIDPGMAARAVDTAARLQRDEFWPGARQGL
                     TQRESEILSFVVNGLSNRAIATKLVIGDETVKSHLRSIYRKLGVSDRTGAVATALREG
                     IYQ"
     gene            1944..>2625
                     /locus_tag="VFC01_14965"
     CDS             1944..>2625
                     /locus_tag="VFC01_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZN80965.1"
                     /translation="MKPRPPNAVRDLVDADRELALLRELIQAASKGPGVEPLAAAAAR
                     MITAATTSDVCFVHVLDDTERSLTLAGATPPFDAEVGKIRLPLGQGISGWVASHREPV
                     VITQDKESDPRYMPFESLRGKDFTSMVSVPMETDPGGLVGVLNVHTVERREFTARDIE
                     LLLVIGRLIAGALHQARLHRQLVARERAHENFVEQVIEAQEMERRRLAGDIHDGISQR
                     LVTLAYRLD"
BASE COUNT          442 a          892 c          857 g          434 t
ORIGIN      
        1 ccggcaccgc tgcggaagat cccgatcctg atcggcggat ccggtcccaa gcgcaccttg
       61 cccgccgtcg cacgccatgc cgacatctgg cacaccttcc tgcccatcga ctcttaccgc
      121 gaggccagtg cccgcgtcgc cgaactggcc gccggattcg ggcgcgccga cagcgatatc
      181 gagcgctcga cgctgtggac cgatccgcag tcggcggacg cctacctggc cgctggagcg
      241 acgttgttcc ataccgaggt ccgcgcgtcg gagggcaaat acgacctcag cacggtggag
      301 aagctggtcg actggcgcga cacgaagcgc tgaacctcta ccggcgatac cgccgcaggg
      361 tatcgagaag ctaggcggca gccctcttgt gcgcggccca ccagctgccg tgcagccgtt
      421 ggcattccac gataccgagc ggctcaccgc tgctgtagtc ggcgcgtacc gccatcgcag
      481 ccggaagatc gggcgtccct tcgccggtga cgacgaccgt ggccagaccc gcgcttgtcg
      541 ccgagcgcag ccctgcggcc gaacccgtga tggcgagggc gttttccgcg ggaatcccga
      601 gctcccacag agcgttttgg tgcgcctcgc gatccggcat cggcttcttc acgtcgtcag
      661 cggtcacgac cgtctcgacc aggccatcgc cgacaaggtg tcggatcagc ggctcggacc
      721 agcttcgtgg gccgttcgtc accacgccca cccagatgcc tgcggcgaac gcgtccatca
      781 ccaggtcgac aagaccgggt cgcggcgcca ggtcggcatc gcggaccatt tcctcgaaca
      841 tcatggtctt ggtcttgtag atctcgtcgg ccagcaactc ggtcagcacg tcgcattctg
      901 tggcgatgcc gcgcttgcgg agctcggcgc agacgcgctg ccgctcgtca ctgagggcca
      961 agagctgccg gtaacggccg acggaccact ggaaatccag gccgtgcgcg gcgaaggccg
     1021 cgttgaacgc cacccgatga ccgtcgcatt cgatgtccgt cagggcgtcg aggtcaaaga
     1081 tcacggcacg tagcggatac acaacgcttt gcgtcggcgc ggtggcatcc caccagaacc
     1141 gacccgcccg ccacgtcttc tcctgcgttg taggcatatg gatagggtgt cgcacgtcac
     1201 aggcattggt cctcccccaa ttgggggatc ggccgggcca ggttctgtta cctcgcgaaa
     1261 tcccagctct aaggtggtgc catgacgtcc ggcagccctc cggtgcgcct tgtgctggtc
     1321 gacgaccacg agatggtcat cgaaggtctc aaggcgatgc ttgcggcgtt cgaggatcgc
     1381 gtactggtgg tgggccaggc ggtcggtgct gagcgagcgc tcagcgtggt cgacgacctc
     1441 gaccccgaca tcgtgctctg cgacgtccgc atgcaggggg ccagcggcct tgacctgtgc
     1501 ctacagctgc gcgagagcga tcccaagcgc aaggtcgtga tgctgtccgt ctacgacgat
     1561 gagcagtacc tcttccaggc gttgcgggtc ggcgcgtcgg gctatctgct gaaaagcatc
     1621 agcagcgacg agctggtccg tcagctcgag ttcgtgcaca aaggcgagac cgcgatcgac
     1681 ccgggcatgg ccgccagagc ggtcgacacc gccgcccggc tgcaacggga cgaattctgg
     1741 ccgggcgcgc ggcaggggct gactcagcgc gaaagcgaga tcttgtcgtt cgtcgtcaat
     1801 ggactgtcga accgggccat cgccaccaag ttggttatcg gcgacgagac cgtgaagtcg
     1861 catctgcggt ccatctatcg caagctcggc gtcagtgacc gcaccggcgc cgtcgcgacc
     1921 gcgctacgcg aaggcattta ccaatgaagc cacgcccacc gaacgccgtc cgcgatctcg
     1981 tcgacgccga ccgcgaactc gcgctgctgc gcgagctgat ccaggccgcg tcgaaggggc
     2041 ccggcgtgga accgcttgcc gccgccgcgg cacggatgat caccgccgcc accaccagcg
     2101 acgtgtgctt cgtgcacgtg ctcgacgaca ccgagcggtc gctcacactc gcgggggcga
     2161 cgcccccctt cgatgccgag gtcggcaaga tcaggctgcc gctggggcag ggcatctcgg
     2221 gttgggtggc cagccaccgc gagcccgtcg tcatcacaca agacaaggaa tccgatccgc
     2281 ggtacatgcc gtttgagtcg ttgcgcggca aggatttcac gtcgatggtg tcggtgccga
     2341 tggagaccga tccgggcggt ctcgtcggcg tcctcaatgt gcacaccgtc gaacggcgcg
     2401 agttcaccgc ccgcgacatc gaactgcttc tggtcatcgg ccggcttatc gcgggcgcgt
     2461 tgcatcaggc ccggctgcac cgtcagttgg tggcgcgcga acgcgcgcac gagaacttcg
     2521 tcgagcaggt catcgaggcc caggaaatgg agcggcgccg cctggccggc gacatccatg
     2581 acggcatctc gcagcggctg gtcaccctgg cctaccggct cgacg
//
LOCUS       DASRJA010000339         6185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3752652,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000339 DASRJA010000000
VERSION     DASRJA010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3752652"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFC01_14970"
     CDS             complement(<1..525)
                     /locus_tag="VFC01_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HZN80966.1"
                     /translation="MAIKVAQIGTGNVGRHALTQLISDPRFELSGVWVSSDSKAGKDA
                     AELAGLDNSTGIAATTDLDAILATQPDCAVYTAMADNRLPDALEDYRRILAAGVNVVG
                     SSAVFLQYPWQTLPDELVTPIEDAAKAGNASLFVNGIDPGFANDLLPLALAGTCQSIE
                     QIRCMEIVDYATYDS"
     gene            complement(537..1328)
                     /locus_tag="VFC01_14975"
     CDS             complement(537..1328)
                     /locus_tag="VFC01_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN80967.1"
                     /translation="MILDKFRLDDQVAVVTGAGRGLGAAMALAFAEVGADVVIAARTQ
                     SQLEEVAEQIRAVGRKAHIVVADLAHPDDTAKLASEAVEALGKLDIVVNNVGGTMPNT
                     LLTTSVKDMKDAFTFNVLTAHALTTAAVPLMLEHSGGGSIINITSTMGRLAGRGFAAY
                     ATAKGALAHYTRQAALDLCPRIRVNAIAPGSILTSALDVVASNDELREPMEKATPMRR
                     LGDPADIAAAAVYLASPAGSFLTGKMLEVDGGLTVPNLDLPIPDL"
     gene            complement(1505..1747)
                     /locus_tag="VFC01_14980"
     CDS             complement(1505..1747)
                     /locus_tag="VFC01_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80968.1"
                     /translation="MSSSGILGKMAAGLGAVAVPVVFAVAGAGQASADPGVCVTGPWG
                     FASACINGPGWVDWSPQWNGGWDDGWGNDNDQGEDD"
     gene            1878..2345
                     /locus_tag="VFC01_14985"
     CDS             1878..2345
                     /locus_tag="VFC01_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZN80969.1"
                     /translation="MSEQRYDLFGEIFRRYFRPAIGMYPHTGGALVERLFTLTDDVPD
                     RLAQTRRAAMAWGKAETPNARTTSELFVITQHGLMYAARMNESRRALYYLATPRGLFD
                     PFVDQIDTAELRDGDVVVVDSGFGRDLASAHPMDAALRRILDEHVALRVDLPA"
     gene            complement(2346..3275)
                     /locus_tag="VFC01_14990"
     CDS             complement(2346..3275)
                     /locus_tag="VFC01_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZN80970.1"
                     /translation="MRSIWKGSIAFGLVNVPVKVYSATEDHDIKFHQVHAKDNGRIRY
                     KRVCEVCGEVVEFRDIAKAYESDDGQTVVITDEDIATLPEERSREIEVLEFVPASDID
                     PMMYDRSYFLEPDGKSSKSYVLLSKTLKETDRVAIVHFALRNKTRLAALRVQDFSKRD
                     VMVVQTLLWPDEIRDPDFPVLDKKVDVKPAELKMASQVVESMTDDFNPDRYHDDYQEQ
                     LHELVQAKLEGGEAFTTEEQPKELDETEDVSDLLAKLEASVKARRSGGGSGGDKPAKK
                     APAKKAAAKKAPAKKAAKKAPAKKAAKKAAAKK"
     gene            complement(3285..4253)
                     /locus_tag="VFC01_14995"
     CDS             complement(3285..4253)
                     /locus_tag="VFC01_14995"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HZN80971.1"
                     /translation="MTATGTPQRALVIGEALIDIVERDGKITGEHVGGSPLNVAVGLA
                     RLGRGVDFLTHIGNDERGRRIADYIEQSGVQLVSGSQTAQRTPTALATLDANGSAQYV
                     FDIDWQLAGTPEVAPPLVAHTGSIATVLEPGCRATAALLDAYHPSATITYDPNVRPAL
                     IEDDDIVRGRIDRWIERCDVIKASDEDMRWIDPNHSPEQTARTWLGLGTSIVAVTMGD
                     QGAFAMCAAGTVRVQAPRVNVVDTVGAGDAFMTGLIDALWSLGVLGAERRPQLARIGV
                     DALTAVVQTAALSSALTVARAGADLPDRATRDAAAARSGPVLPHPG"
     gene            complement(4250..5671)
                     /locus_tag="VFC01_15000"
     CDS             complement(4250..5671)
                     /locus_tag="VFC01_15000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HZN80972.1"
                     /translation="MKLNESMLAQIPIDKPSYDRDEISIGIVHFGVGGFHRAHQAMYV
                     DRLLQAGLAKEWGICGVGVLPADRKMADVMTAQNGLYTLLSENPDGSRDARVIGSIID
                     YRYAPDNPEAVVELLAAPSTRIISLTITEGGYNIDSLGDGVNVFGLVADALARRRDRG
                     IASPTIVSCDNIEGNGEVARQAFTTYAERVHPGLGEWIGAHTRFPNSMVDRITPVTTP
                     DVIAVLQSQFGVEDQWPVAAEPFTSWVLEDDFSDGRPPLEQADVLLVDDVTPYELMKL
                     RLLNASHQSLCYFAYLAGYRLVHDAAGDPLLAEFLREYMDSEATPTLKPVPGIDLPDY
                     KRTLIERFANPGVKDTIARLCFGSSDRIPKWLLPVVRANLASGGPVRLSAATVASWAR
                     YAEGIDETGEPIDVQDQLADTLVPLARSQHENPTAFIENTQVFGDLASQPRFVEAYLW
                     ALESLHRDGARATLETLLRKETT"
     gene            complement(5668..>6185)
                     /locus_tag="VFC01_15005"
     CDS             complement(5668..>6185)
                     /locus_tag="VFC01_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN80973.1"
                     /translation="DGGSRVLITGAGPIGIMVAQVARAYGATDIVVSDPDESRREQAT
                     VFGATQVLDPTVEPIGDLGVDSFIDASGAPQAVVDGIRAVRPAGTVVLVGSGAESMEL
                     PTQLIQNRELVLTGVFRYANTWPTAIALVESGRVDLDAMVTAHFPLERAAEALDSDRT
                     PGSVKSVVAVS"
BASE COUNT          986 a         2047 c         1991 g         1161 t
ORIGIN      
        1 actgtcgtag gtggcgtagt cgacgatctc catgcaccgg atctgctcga tgctctggca
       61 ggtgccggcc agcgcgagcg gaagcaggtc gttggcgaaa ccggggtcga tgccgttgac
      121 gaacaggctt gcgttgcctg ccttggcggc gtcctcgatc ggcgtgacga gctcgtcggg
      181 cagggtctgc catgggtatt gcaggaacac cgcgctgctg ccgacaacgt tgacccctgc
      241 tgcgaggatt ctgcggtaat cctccagcgc gtcgggcagg cggttgtcgg ccatcgcggt
      301 gtagacggcg cagtccggtt gtgttgcgag aatggcatcg aggtcggtgg tggcggcgat
      361 gcccgtcgaa ttgtcaagtc ccgcaagctc tgcggcgtcc ttgcccgcct tggaatcgct
      421 ggacacccag accccgctga gctcgaaccg cgggtcgctg atcagttggg tcagcgcgtg
      481 tcggccgacg ttgcccgtgc cgatctgtgc gactttgatg gccatcggtg agctcctcac
      541 aggtccggga tgggtagatc gaggttgggg acggtcaggc cgccgtcgac ctcgagcatc
      601 ttgccggtca ggaagctgcc cgcaggcgac gcgaggtaca ccgcggcggc cgcgatgtcg
      661 gccggatcgc cgagtcgtcg catcggcgtt gccttttcca tcggctcgcg tagttcgtcg
      721 ttggaggcga ccacgtcgag cgctgaggtg aggatggagc ccggtgcgat cgcgttgacc
      781 cgaatccgtg gacagagatc gagggccgcc tggcgggtgt aatgcgcgag cgcgcccttg
      841 gcggtggcgt atgcggcgaa tccgcggccg gccagccgtc ccatggtcga ggtgatgttg
      901 atgatgctgc cgccaccgga gtgctcgagc atgagcggga cggcggcggt ggtcaacgcg
      961 tgagccgtga gcacgttgaa ggtgaaggcg tccttcatgt ccttgaccga cgtggtcagc
     1021 agcgtgttgg gcatggtgcc gccgacgttg ttgacgacga tgtctagttt cccgagcgcc
     1081 tcaaccgctt cgctggccaa cttggcggtg tcgtcggggt gcgcgaggtc ggcgacgacg
     1141 atatgcgcct ttcggccgac cgccctgatc tgctcggcga cctcctcgag ctgcgactgc
     1201 gtgcgcgccg caatcaccac gtccgcaccg acttccgcga atgccagcgc catcgccgcg
     1261 ccgagtccgc ggcccgcgcc ggttaccacc gcgacttggt cgtccagcct gaatttgtcc
     1321 agaatcacgt gcgcctctct ctctcgccgg gcgaaccgta gcaggggtta gcgcgacatg
     1381 ggaggtgttt ttgaaacacg ttctagtttt ctcaggaaga ggcgtgtgcc ttcttggaac
     1441 cgtcatcaat tgttgacggc tccagaaaga gtgttggtcc aggccgggct attgagcgac
     1501 ggcgctagtc gtcttcacct tggtcgttgt cgtttcccca accgtcgtcc cacccaccgt
     1561 tccactgcgg gctccagtcg acccatccgg gaccgttgat gcatgcggat gcgaaccccc
     1621 aaggaccggt tacgcagaca ccgggatctg ctgacgcttg ccccgctccc gcaaccgcga
     1681 acaccactgg caccgccacg gcgcccaatc ccgccgccat ctttccgagg attcctgacg
     1741 aggacataac tgtgcctcca atccctgcgc caagagccta gccctctttg gggtcatggc
     1801 ggcaccgcca aactcgtgct ccgcagacgg tttcgggcgc acaattgggg agcacgttcg
     1861 aaatctgggg agccgcgatg agcgagcaac ggtacgacct tttcggtgag atcttccggc
     1921 ggtacttcag gcccgcgatc ggcatgtatc cgcatacggg tggcgcactc gtcgagaggt
     1981 tgttcacgct taccgacgac gtcccggatc gattggccca gacccggcgt gccgcgatgg
     2041 catggggcaa agccgagacc ccgaacgcca ggaccacttc tgaactcttc gtgatcaccc
     2101 aacatggact gatgtatgca gccaggatga acgagtcgcg ccgggcgctg tattacctgg
     2161 ccacgccacg tgggttgttc gatccgttcg tcgatcagat cgacaccgcc gaactgcgtg
     2221 acggcgatgt tgttgtggtg gacagtgggt tcggccgtga cctcgcatcg gctcatccga
     2281 tggacgccgc gctgcgccgg atactcgacg agcacgtggc gctgcgggtg gatctgcccg
     2341 cgtagctatt tcttcgctgc ggccttcttg gcggccttct tcgcaggtgc ctttttcgca
     2401 gccttcttgg ccggcgcctt cttggccgca gctttctttg cgggcgcctt cttcgccggc
     2461 ttgtcgccgc cggaaccacc gcccgaccgg cgcgccttca cgctggcctc cagcttggcc
     2521 aacagatccg agacgtcctc ggtctcgtcg agctccttcg gctgctcttc ggtggtaaac
     2581 gcttctccac cttcgagttt cgcctggacc agctcgtgca gctgttcttg atagtcgtcg
     2641 tggtatcggt ccgggttgaa gtcgtcggtc atcgactcca ctacctggct cgccatcttc
     2701 agctcggcgg gtttgacgtc aaccttcttg tccagcaccg ggaaatcggg atcgcgaatc
     2761 tcgtcgggcc acaacagcgt ctggaccacc atcacgtcgc gcttggaaaa atcctgcacc
     2821 cgcagcgccg caagccgcgt cttgttgcgc aacgcaaagt gcacgatcgc gacccggtcg
     2881 gtctccttca acgtcttcga caacagcacg taggacttcg acgacttccc gtcgggctca
     2941 aggaagtagc tgcggtcgta catcatcgga tcgatgtcgc tcgccggaac aaactccagc
     3001 acctcgatct cccggctgcg ttcctccgga agggtagcga tgtcctcgtc ggtgatcacc
     3061 accgtctgac cgtcgtcgga ttcgtaggcc ttcgcgatgt cgcgaaactc gacgacctcg
     3121 ccgcacacct cgcaaacgcg cttgtaccgg atgcgtccgt tgtccttcgc gtggacctga
     3181 tggaacttga tgtcgtggtc ttcggtcgcg ctgtagacct tgaccgggac gttcaccagg
     3241 ccgaaggcga tcgaaccctt ccatatggaa cgcatcagcc caggctagcc gggatgaggc
     3301 aacaccgggc ccgacctggc cgctgcggcg tcccgggtcg cccgatcggg caggtcagcg
     3361 cccgcacgcg ccaccgtcaa cgccgaggag agcgccgccg tctgcaccac cgcggtcagc
     3421 gcatcgacac cgatccgggc cagttggggg cgacgctcgg cgccgagcac acccagcgac
     3481 cacagcgcat cgatcaggcc cgtcatgaac gcgtcgcctg cccccacggt gtccacgacg
     3541 tttacccgag gcgcctgcac ccgcaccgtc cccgccgcgc acatcgcgaa cgcgccttgg
     3601 tcacccatcg tcaccgcgac gatcgacgtg cccagcccga gccacgtccg ggcggtctgt
     3661 tcgggggagt ggttcgggtc gatccagcgc atgtcctcgt cgctggcctt gatcacgtcg
     3721 cagcgttcga tccagcggtc gatgcggccg cgcacaatgt cgtcgtcctc gatcagcgct
     3781 gggcgcacgt tcgggtcgta cgtgatggtc gccgaggggt gatacgcgtc gagcagggcg
     3841 gcggtggccc ggcaacccgg ttccagtacc gtcgcgatcg accccgtgtg cgcgacgaga
     3901 ggcggcgcca cctccggtgt tcctgccagt tgccagtcga tgtcgaacac atactgcgcc
     3961 gagccgttgg cgtcaagcgt cgccagcgcc gtgggtgtgc gctgcgccgt ctgactgccc
     4021 gaaaccagtt gcacaccaga ctgttcgatg taatcggcga ttcggcggcc gcgttcgtcg
     4081 ttgccgatgt gggtgaggaa gtcgacgcca cggcccagcc gcgcgagtcc gaccgcgacg
     4141 ttcaaggggc tgccaccgac gtgctcgccg gtgattttcc cgtcccgctc gacgatgtcg
     4201 atcagcgcct cgccgatcac cagcgcgcgc tgcggtgtgc cggtcgcggt catgtcgtct
     4261 ccttacgcag cagcgtctcc agggtcgcgc gggcaccgtc gcggtgcagc gattccaacg
     4321 cccacaggta tgcctcgaca aaccgtggct gcgacgcgag atcgccgaat acctgggtgt
     4381 tttcgatgaa tgctgtgggg ttctcgtgct gagaacgggc cagcggcacg agagtgtcgg
     4441 cgagttggtc ctggacgtcg atgggctcgc cggtctcgtc gatcccttcg gcataccgcg
     4501 cccagctggc gacagtggcg gccgacaacc ggacgggacc acccgacgcg aggtttgccc
     4561 gcaccacggg cagcagccat ttcgggatgc ggtccgacga tccgaagcac aatcgcgcga
     4621 tggtgtcctt tacgccgggg ttcgcgaatc gttcgatcag cgtgcgcttg tagtcgggca
     4681 ggtcgatgcc cggcaccggc ttgagcgtcg gcgtggcctc ggaatccatg tactcgcgca
     4741 ggaattcggc caacagcggg tcgcccgccg cgtcgtgcac caaccgatag ccggccaggt
     4801 aggcgaagta gcacaggctc tgatggctgg cgttgagcag gcgcagcttc atcaactcgt
     4861 agggcgtcac atcgtcgacc aacaaaacgt ccgcctgctc gagcggcgga cggccgtcgg
     4921 agaagtcgtc ctcgagtacc cacgacgtaa acggttcggc ggccaccggc cactgatcct
     4981 cgacaccgaa ttggctttgc agcacagcga ttacatcggg cgtggtgacc ggcgtgatgc
     5041 ggtccaccat cgaattcgga aaccgcgtgt gtgcgccgat ccactcgccc aggccggggt
     5101 gtacacgttc ggcgtaggtg gtgaacgcct gccgcgcgac ttcgccgttg ccttcgatgt
     5161 tgtcgcacga cacgatcgtc ggcgaggcga tgccgcggtc gcgccgtcgt gccaacgcat
     5221 cggccaccag accgaacacg ttgacgccgt cacccaggct gtcgatgttg tagccgccct
     5281 cggtgatggt cagcgagatg atccgggtgc tcggcgcggc gagcaattcg acgaccgcct
     5341 ccggattgtc gggcgcatag cggtagtcga tgatcgaccc gatcacccgc gcatcgcggc
     5401 tgccgtcggg gttttccgac agcagtgtgt acagcccgtt ctgcgcggtc atcacgtcag
     5461 ccatcttgcg gtcggcgggc agcacgccga cgccgcagat gccccactct tttgccagcc
     5521 ccgcctgcag caggcggtcg acgtacatcg cctggtgtgc gcggtgaaat ccaccgacgc
     5581 cgaaatgcac tatgccgatt gagatctcgt cgcggtcata gctcggcttg tcgatcggga
     5641 tctgcgccag catggactcg ttcagcttca tgacaccgcc accacgctct tgacgctacc
     5701 cggcgtgcgg tcggagtcga gggcctcggc ggccctctcc aacgggaagt gcgcggtcac
     5761 catcgcgtcg agatccaccc gaccggactc gacgagcgcg atggccgtcg gccaggtatt
     5821 ggcatagcgg aacaccccgg tcagcaccag ctcccggttc tggatcagct gcgtcggcag
     5881 ctccatcgac tcggcgcccg aaccgacgag caccacggtg cctgccggcc gcaccgcgcg
     5941 gataccgtcg acgacggcct gcggtgcgcc ggaggcgtcg atgaacgaat cgacacccaa
     6001 atccccgatc ggttcgacgg tcgggtcaag aacctgggtc gccccgaaca cggtcgcctg
     6061 ctcgcgtcgg gactcgtcgg ggtcgctgac aacgatgtcg gtggcgccgt acgccctggc
     6121 gacctgggcc accatgatgc cgatcggccc tgcacccgtg atcagcacgc gcgaaccgcc
     6181 gtcga
//
LOCUS       DASRJA010000340        18678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2085260,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000340 DASRJA010000000
VERSION     DASRJA010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18678
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2085260"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VFC01_15010"
     CDS             complement(<1..1160)
                     /locus_tag="VFC01_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HZN80974.1"
                     /translation="MTYEHMRAAHLHAVQAAMEDHIARLDWQRERIDRYRDQRLRMLL
                     SYALERSPFHARRLAGIDPARATVADLAGVPMMTKAEAQDCWDDVVTDRGLNRERAES
                     VLAEQEWYSYTQDDYQVFSSGGSSGVRGVYVWDWAFFVSVTCLAWRTQARTDLQTPQK
                     GPTRLAVLEAGMPPHASTPLFDVPTAAGMETVVIAAGAPFDEVLVAVAAARPTHLVGY
                     PSVIGRLARAALAGQLDCSPVRVSTNSEPLLDEDRQAIVEAWHVPIHNLWGSTEIGVQ
                     AVGCGIGEGLHICEDEVVLERVDQNGVPVGPDEPAVRTLATGLANRTFPFIRYDLGDE
                     VTLLPDPCECGSAYARVADIGGRRDDDFRYGSVTVPAITFRHVLGTDPHISE"
     gene            1298..2287
                     /locus_tag="VFC01_15015"
     CDS             1298..2287
                     /locus_tag="VFC01_15015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN80975.1"
                     /translation="MRFGFFIPQGWRLDLVDIPTDKHWQVMHDLAAYADGGAWDSLWV
                     YDHFHTVPVPTHEATHEAWSLMSAYAATTSRIKLGQMCTSMGYRNPVYLAKVAATADI
                     ISGGRVQMGIGGGWYQHEWEAYGYGFPSAGVRLGMLDEGVRIMRDAWRDGEVILDGKH
                     YQVNGAIVEPKPLQDRGLPLWIAGGGEKVTLRIAAKYAQYTNFTSEPDGFAHKSRVLA
                     DHCRDVGTDYDAIVRSANFNAVIGESEADVKERVARLRARQVAKANEAAVDGMLAMAT
                     SPDAASGTPEQIVEKLKRMRELGCEYAILYFPEAAYDRSGIELFEREVIPALS"
     gene            2351..2947
                     /locus_tag="VFC01_15020"
     CDS             2351..2947
                     /locus_tag="VFC01_15020"
                     /inference="COORDINATES: protein motif:HMM:NF039175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EspA/EspE family type VII secretion system
                     effector"
                     /protein_id="HZN80976.1"
                     /translation="MGLFDFVHDVVDVVSDPAEAVEKTAEWASDIAEDLGLDKIARVA
                     RSVGKFAGKLAIAPTPILEGGQKVIEEMIEAAGGDNDPERGESFGDGSRKFKAQLQTL
                     NKAHPGQLWEGGTASKAYDSRTDEQENRVTTLSEADIEIATVLTAEAHQLEVLRQVLH
                     HRPAPVETSHRCCRSSPRSVSPHIYDDRASMGRPIAGI"
     gene            complement(2944..3300)
                     /locus_tag="VFC01_15025"
     CDS             complement(2944..3300)
                     /locus_tag="VFC01_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HZN80977.1"
                     /translation="MRDTELLRGVLEHWKAGIDAHDPQAVAAVFTEDAIFQGLRPYSV
                     GRDGVAEYYERQPLGMTVTYRVLETRRPTEELVLGYLRADFAFPDRPTVSVNIGVAVT
                     HDNGWKILHYQASRLD"
     gene            complement(3287..4000)
                     /locus_tag="VFC01_15030"
     CDS             complement(3287..4000)
                     /locus_tag="VFC01_15030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN80978.1"
                     /translation="MSNSQRVAIITGASQGIGKGLIKAYRKLGYAVVANSRTIAESDD
                     PMVVTVPGDIGQPGVGQRIADAAMQNFGRVDTVVNNAGIFIPKPFTEYTDDDYDAIMG
                     VNLRGFFEVSRAAVAAMLTREGGGHIVNVSTSLVDHANSQVPSALASLTKGGLNAVTK
                     SLAIEYAGRGIRANTVALGVIRTPMHDPSSHEALAKLHPVGRLGEIDDVVDAVLYLEN
                     ASFVTGEILHVDGGQSAGH"
     gene            4127..4297
                     /locus_tag="VFC01_15035"
                     /pseudo
     CDS             4127..4297
                     /locus_tag="VFC01_15035"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(4965..6137)
                     /locus_tag="VFC01_15040"
     CDS             complement(4965..6137)
                     /locus_tag="VFC01_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007735604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HZN80979.1"
                     /translation="MHTHWAHVGRGAAGLGAAMGIGRFAYTPILPLMTTQAVLTPQAA
                     GTLATANYVGYLAGALAGTASPRLARSTVAWRASLAVLVISLAAMPLAPNIIGWLLLR
                     TVAGFASAVAFVIAVNSMLDHLPPHLPGWGFGGIGMGIALSGALVLTLPTEAGWQGAW
                     WTAAAVAAVLGAGAWAMRGTSRPAAAPPSSPRRANRWFAVLFVSYTLEGIGYIVAGTF
                     LVAAIKQNSSGWLGSGAWLVVGLAAVPSAALWAWLCARWSHPTLLAGALLLQAFGIAL
                     PALAPGPVAALTGAVLFGATFIGVSTMALAAGRLLGFPGAVALLTSGYSVGQILGPVA
                     VTPLLRHGFSLALLVAAAVVLLSAMVAGLLRFGFPSAGADPQPAASRRHPILVRRG"
     gene            6321..6653
                     /locus_tag="VFC01_15045"
     CDS             6321..6653
                     /locus_tag="VFC01_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80980.1"
                     /translation="MLSISREVDLEPHRPVWVPRPRLVSQRADGWARPAHPADIAQAR
                     LFDAALLGEIAELEALVASMEKRWLRRCERGIDDENRPPENLVRMRGRVSEAQQLLDA
                     LRERFPSK"
     gene            complement(6684..7520)
                     /locus_tag="VFC01_15050"
     CDS             complement(6684..7520)
                     /locus_tag="VFC01_15050"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZN80981.1"
                     /translation="MTTNTILVIGSTGKTGKRVVNQLEKRGIAVRHGSRSADIPFDWD
                     NPQTWAAALAGVDKVYLTYYPDLAAPGSVDGVSTLMQMAKDAGVSRMVLLSGRNEAEA
                     EKAECVVMASGLEWTIVRCAFFAQNFNEGAWLDEVLAGTISLPVDRVQEPFVDADDIA
                     DVVVAALTDDRHVGQLYELTGPRLLTFRDAVAEIGAAAGRDISFISVPIDDYTTMLTE
                     YGLPQDFIWLLNHLFTEVLGSKAELADGVQRALDARPRTSLTTPARPPRPVSGPRQFE
                     SR"
     gene            7605..7949
                     /locus_tag="VFC01_15055"
     CDS             7605..7949
                     /locus_tag="VFC01_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN80982.1"
                     /translation="MEKISLTALARQHLESARTASSGRSAHTVYGGHEHSLRQTLMAM
                     TAGSTMDDHESPGEATLQVLHGRVRVTNSQSGWDGSPGDHIVLPRTRHGLHALEDSVV
                     LLTVAKAVGPHV"
     gene            7990..8409
                     /locus_tag="VFC01_15060"
     CDS             7990..8409
                     /locus_tag="VFC01_15060"
                     /inference="COORDINATES: protein motif:HMM:NF017309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HZN80983.1"
                     /translation="MSLSGGLVGCQRGPEVVTSIVFDGETRSMTTTDVSCTKQPDGGL
                     VILVQDKRRHMVRLQLSQQARLVVHKAGLRYDDMVGFVADPREVTATKVDDTFTFSGR
                     MPPNPGESQWHTFKIQTSCPGYVDAPPPRLDPGIGAP"
     gene            complement(8416..8772)
                     /locus_tag="VFC01_15065"
     CDS             complement(8416..8772)
                     /locus_tag="VFC01_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003944926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN80984.1"
                     /translation="MTVLRVMPIFTVSDVDAAREAYIRVLGLREVMNLGWVVTLAGDE
                     LRHQISLMTKDATAPVNPNVSIEVDDVDAAYQAAVDAGLQIVHPLSDEEWGVRRFFFA
                     DPAGNVVNVLSHRHDV"
     gene            complement(8769..10418)
                     /locus_tag="VFC01_15070"
     CDS             complement(8769..10418)
                     /locus_tag="VFC01_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HZN80985.1"
                     /translation="MTDGPLIVQSDKTVLLEVDHELAGAARAAIAPFAELERAPEHIH
                     TYRITPLALWNARAAGHDAEQVVDALVSFSRYAVPQPLLVDIVDVMARYGRLQLVKHP
                     AHGLTLVSLDRAVLEEVLRNKKIAPMLGARIDDDTVMVHNSERGRVKQMLLKIGWPAE
                     DLAGYVDGEKHPIALDQADWHLRDYQEMAAESFWEGGSGVVVLPCGAGKTLVGAAAMA
                     KAGATTLILVTNTVAGRQWKRELINRTSLTEDEIGEYSGEKKEIRPVTIATYQVITRR
                     TKGEYRHLELFDSRDWGLIIYDEVHLLPAPVFRMTADLQSRRRLGLTATLIREDGREG
                     DVFSLIGPKRYDAPWKDIEAQGWIAPAECIEVRVTMTDNERMLYAVAEPDERYKLCST
                     VHTKIAVVKSILEKHQGDQTLVIGAYLDQLDELGQELNAPVIQGSTKTAEREALFDAF
                     RRGEVSTLVVSKVANFSIDLPEANVAVQVSGTFGSRQEEAQRLGRLLRPKHDGGGAMF
                     YSVVSRDSLDAEYAAHRQRFLAEQGYGYVIKDADDLLGPAI"
     gene            complement(10456..11895)
                     /locus_tag="VFC01_15075"
     CDS             complement(10456..11895)
                     /locus_tag="VFC01_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZN80986.1"
                     /translation="MTTLLSEAAQAPGGYRHGLSLLHGWLPPTIWTVAAVVLVVAIEW
                     RSRRWRLVWLPVALVVGLILAAWAHWYVDSEGLAGDPPPSSLLVWVGLTGVALAVAVL
                     GWRSSRWRRRIVSLVAVPLCVLSAGVALNLWVGYFPTVQAAWNELTAGPLPDETDLNT
                     VMAMRGKGIPAHGALVPVTIPADASGFKHRTEFVYLPPAWFAANAPELPLVMMIAGEF
                     NTPSDWPRTGNAIATIDTFAAAHGGNAPVLVFVDVGGSFNNDTECVDGPRGNVADHLT
                     KDVPPYVSSTFGVQNANWGVVGWSMGGTCAVDLTVMHPQLFSTFVDIAGDMGPNAGTK
                     EQTIARIYGGDAAKWAIYDPTTVITKHGPYQGASGWFAISSDAPTPRKGGYANPNATG
                     LGGQDAAGNPGDQNDAANSLCSLGRANGIACAVVASPGKHDWPFAMDVFKDSLPWLAG
                     QIGTPEVPKIPLPGAGGRPSAAPMPTAAH"
     gene            complement(11949..14198)
                     /locus_tag="VFC01_15080"
     CDS             complement(11949..14198)
                     /locus_tag="VFC01_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HZN80987.1"
                     /translation="MTETTPGVPLGAWLAELDDERLIRLLELRPDLTQPPPGTIAALA
                     ARAQARQSVKAATDGLDFLRLAVVDALLVLHADTAAVPLTKLLALIGDKASEDTVTAA
                     VDDLRERALVWGDATVRVAAEATAGLPWYPGQATVEDSEQTDIAARLEALDAPQLELL
                     QRLLEGSPMGRTRDAAPGTPPDRPVQRLLAAGLLRQVDSETVILPRLVGQVLRGELPG
                     PVQLRQPDPTESTTAVRDVDAVAAGAAIDLLREVELVLETLGATPVPELRSGGLGVRD
                     VKRLAKTTGVEEARLGLLLEVSAAAGLIAAGMPEPDPHDGVGPYWAPTVAADRFVESP
                     TASKWHLLASTWLDLPGRPSLVGNRGPDGKLYAALSDSLFSTAAPLDRRLLLDVLADL
                     PPGAGVDATSASRAMIWRRPRWAVRLQPEPVGNLLTEAHALGMVGRGAIASPARNMLA
                     GDPPDDVVAAMEKVLPAPIDHFLLQADLTVIVPGPLQRDLAEKLASVATVESAGAAMV
                     YRVTEASIRRALDTGRTASELHTLFSTHSKTPVPQGLTYLIDDVARRHGQLRVGMAQS
                     FVRCEDPALLAQAVAAPAVELLEIRLLAPTVAVSQAPISEVLAALRGAGFAPAAEDST
                     GTIVDIRARGARVPAPSGRRRVHWPQTPTTQTLAAIVAVLRKVASASFGNMRLDPAVA
                     ISQLQEAAHMQSSVVIGYVDPAGVATQRVVAPINIRGGQLTAYDPASGRVREFAIHRV
                     TSVVSADSA"
     gene            14264..14455
                     /locus_tag="VFC01_15085"
     CDS             14264..14455
                     /locus_tag="VFC01_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80988.1"
                     /translation="MADKKQKNRYVDPGWPELDGDDDHAVSEAATDRTGALSPFGELV
                     FPQPADELPYIHPITVVNK"
     gene            14458..14937
                     /gene="moaC"
                     /locus_tag="VFC01_15090"
     CDS             14458..14937
                     /gene="moaC"
                     /locus_tag="VFC01_15090"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HZN80989.1"
                     /translation="MTDPARRLSHLDESGAAHMVDVTGKDATKRTAVAAGTFHTTADV
                     VALIASGGLPKGDAVATARVAGILAAKRTSDLVPLCHPLALTGVDVDFEIGDDSIGIT
                     ATVRTTDRTGVEMEALTAVSVAALTLYDMIKAVDPAAHIDDIRVLRKEGGKTGTWTR"
     gene            14934..15413
                     /locus_tag="VFC01_15095"
     CDS             14934..15413
                     /locus_tag="VFC01_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HZN80990.1"
                     /translation="MTRSGRVVIASTRAAAGVYEDRTGPIIVDWLNGQGITTPAPVVV
                     PDGSGVVKALFTAIGENVDVIITSGGTGISPTDGTADATAEIVDYQIPGLADAIRRSG
                     LPHVPTSVLSRGVCGVRGRTLIVNLPGSTGGVKDGLGVLDDVLEHALDQLSGKDHTR"
     gene            15410..15838
                     /locus_tag="VFC01_15100"
     CDS             15410..15838
                     /locus_tag="VFC01_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HZN80991.1"
                     /translation="MNAVVLRVELTEQPIELSEYEALVAHESAGAIVGFAGVVRDHDD
                     GRGVTRLEYSAHPKAQQTLADVAAEIAADSPGVRAIAVSHRIGTLQIGDAALVAAVAA
                     DHRSAAFETCARLVDTVKARLPVWKHQFFADGSDEWVNSA"
     gene            complement(15843..17069)
                     /locus_tag="VFC01_15105"
     CDS             complement(15843..17069)
                     /locus_tag="VFC01_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HZN80992.1"
                     /translation="MSGRHRKPTTSAVNVAKIAFTGAVIGSGSLALAGQAGAATDGEW
                     DQVAKCESGNNWAINTGNGYQGGLQFSPSTWSSHGGGEYAPSAHMATKEEQIAVAERV
                     LATQGKGAWPVCGRGLSGATPRNVIEEPQALDAPAVNGELPPPPPLDPFAPPAPPEAA
                     PIDELSAPLPDAPPPPPAPLDAPLPPPPAPVEGQIVDASLDAPLPPPPPPVDAPPIED
                     TIVAAADNWDVAPAPVDQPEVWALHAPAPLDPVLPPLPPAPPAPPAPPAPGAVAAPAP
                     DPLAPLNAVNVPAPAFDAANQAMTDPAAALPTLPDGVPHLASPDALPPGSTMDPAANG
                     ADGPNVSYLKDLWQAVQNHDISGKEALIMGLAQRGMNTPYPEQGPGPNVPVTPAPPAD
                     PAAPPAPDAPPILPPA"
     regulatory      complement(17079..17251)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(17500..17754)
                     /locus_tag="VFC01_15110"
     CDS             complement(17500..17754)
                     /locus_tag="VFC01_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZN80993.1"
                     /translation="MAVKVTVRYFAAARAAAGFDDEIIGVAPGTTVGALVQGLGERDT
                     ELAKVLSRCSYLCDGIAVRDLGMTLSDAQTVDVLPPFAGG"
     gene            complement(17756..>18678)
                     /gene="moaA"
                     /locus_tag="VFC01_15115"
     CDS             complement(17756..>18678)
                     /gene="moaA"
                     /locus_tag="VFC01_15115"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZN80994.1"
                     /translation="LRCTYCMPAEGLDWLPGEQLLRTDELTRLLRIAVIRLGITSVRF
                     TGGEPLVAKHLEDVVAATAGLRPRPEITLTTNGIGLDKRAARLKQAGLDRINVSLDTV
                     DAARFAHITRRDRLHDVLRGLEAAKAAGLAPVKVNAVLDPVTGLDDAVALLRFCLDQG
                     YQLRIIEQMPLDAGHEWKRGRAIKADDVLDALQRHFTLTPDRAPRGSAPAELWDVNGG
                     LGKVGIIASVSHAFCAACDRTRLTADGQIRNCLFAQHETDLRRLMRAGADDDALAAAW
                     RATMWVKAAGHGINDPGFVQPDRPMSAIGG"
BASE COUNT         3059 a         6236 c         6288 g         3095 t
ORIGIN      
        1 tattccgaga tgtgtgggtc ggtgcccagt acatgccgga aagtgatggc gggcaccgta
       61 actgagccgt agcggaagtc gtcgtcgcgt cgcccgccga tgtctgccac gcgcgcatag
      121 gcgctcccgc actcgcaggg atccggtagc aatgtcacct cgtcgccgag gtcataacgg
      181 atgaacggaa acgtccgatt ggccagtccg gtggcgagcg tccgcaccgc gggctcatcg
      241 ggacccaccg gcacgccgtt ctggtccacg cgttccagca ccacctcgtc ctcgcagatg
      301 tgcaggccct cgccaatccc gcaaccgacc gcctgcaccc caatctcggt ggatccccac
      361 aggttgtgga tcggtacgtg ccaggcttcg acgattgcct ggcgatcttc gtcgaggagt
      421 ggctcggagt tggtgctcac ccgtaccggg ctgcagtcca actgtcctgc cagcgccgcg
      481 cgggcaagcc gtccgatgac agacggatag ccgacgaggt gcgtgggtcg ggcggccgcc
      541 accgcgacca acacctcatc gaacggtgcc cctgccgcga tgacgacggt ttccatgccc
      601 gcagcggtcg gcacgtcgaa caacggcgtg ctggcgtgtg gcggcattcc cgcttccagc
      661 actgccagac gcgtcgggcc cttctgcggc gtctgcaggt ccgttcgcgc ctgcgtccgc
      721 catgccaggc atgtgaccga cacgaaaaac gcccaatccc atacgtatac gcccctgacc
      781 ccgctggagc cgcccgagct gaacacctgg tagtcgtcct gcgtatacga ataccattcc
      841 tgttccgcca gaacgctttc cgcgcgctcg cgattcagcc cgcggtcggt gaccacatcg
      901 tcccagcagt cctgcgcctc cgccttcgtc atcatcggta cgccagccag atccgccacc
      961 gtggcgcggg ccggatcaat gccggccaat cgccgggcat ggaagggcga gcgttccagg
     1021 gcgtagctca gcagcatgcg cagccgttga tctcgatagc ggtcgatgcg ctcgcgctgc
     1081 caatccagac gtgcgatgtg atcctccatc gccgcctgga ccgcatgaag gtgcgccgcg
     1141 cgcatgtgct cgtaggtcat ttcgtcccga tcaccatatt tggggtggac actgcgtatt
     1201 acccaatcgt caatttcggg gcgggccacc atgaccgact tgtgccacct accggctggc
     1261 ggcctccacc gtcgctgacg gcatagagtc gagggtcatg cgctttggat tcttcatccc
     1321 gcaaggctgg cgactggacc tcgtcgacat tcccaccgac aagcactggc aggtgatgca
     1381 cgatctcgcc gcctacgccg atggcggcgc gtgggactcg ctgtgggtct acgaccattt
     1441 ccacaccgtg ccggtgccga cgcatgaggc cactcacgag gcctggtcgc tgatgtcggc
     1501 gtacgcggcg accacgtcgc ggatcaagct cggccagatg tgtacgtcga tgggctatcg
     1561 caacccggtg tatctggcca aggtggccgc caccgcagac atcatctccg gcggccgggt
     1621 ccagatgggt atcggcggtg gctggtacca acatgagtgg gaggcctacg gctacgggtt
     1681 cccgtccgcc ggtgtccggc tcggcatgct cgacgagggt gtccggatca tgcgcgacgc
     1741 ctggcgcgac ggcgaggtca ttcttgacgg caagcactat caggtcaacg gcgcgatcgt
     1801 agagccaaaa ccgttgcagg acaggggtct tccgctgtgg atcgccggcg gcggtgagaa
     1861 ggtgacgctg cggatcgccg ccaagtacgc gcagtacacc aacttcacct ccgaacccga
     1921 tggctttgcg cacaagtccc gggtgctggc cgatcattgc cgcgacgtcg gcaccgacta
     1981 cgacgcgatc gtgcggtcgg ctaacttcaa cgccgtgatc ggcgagtcgg aggccgacgt
     2041 caaagagcgg gtcgcgcggc tgcgcgccag gcaggtcgcc aaggcgaacg aggccgccgt
     2101 cgacggcatg ctggccatgg ccacctcgcc cgatgccgct agcggcacac cggagcagat
     2161 cgtcgagaag ctaaagcgga tgcgcgaact cggctgtgag tacgcgatct tgtacttccc
     2221 cgaggctgcc tacgaccggt cgggtatcga gttgttcgaa cgcgaggtca tccccgcgtt
     2281 gagttgaatt cgtgcacaga gcacctgcgg cgcgctgtta gcctgacccg gacggaaagg
     2341 tgcggcaggc atggggctct tcgactttgt ccatgacgtg gtcgacgttg tctcggaccc
     2401 tgccgaagcc gtcgagaaga cggccgaatg ggccagcgat atcgcggaag atctcgggct
     2461 ggacaagatt gcccgcgtcg cgagatcggt cgggaagttc gcaggcaagt tggcgatcgc
     2521 gccgacaccg atcttggagg gtggccagaa ggtcatcgaa gagatgatcg aggcggcagg
     2581 aggcgacaac gaccccgaac gcggagagtc attcggggac gggtcgcgaa agttcaaggc
     2641 gcagcttcaa accctgaaca aggcgcatcc gggtcaacta tgggagggcg gtaccgcgtc
     2701 gaaggcctac gatagccgca ccgacgaaca agaaaaccgc gtgacgacgt tgtccgaggc
     2761 ggacattgag atcgccacgg tgctgactgc tgaagctcat cagctcgagg tgctacgcca
     2821 agtgttgcat catcggccgg caccggttga gaccagccac cgctgttgtc gttcgtcacc
     2881 gcgttccgtc tcgccgcata tctacgacga tagggcatcc atgggacgtc ccatcgcggg
     2941 gatttagtcg agccgcgacg cctgatagtg gaggattttc cagccgttgt cgtgcgtgac
     3001 cgccacgccg atgttgacgc tgaccgtagg ccggtccggg aatgcgaagt cggcgcggag
     3061 gtagccgagc accaattcct ctgtgggcct gcgcgtttcc agcacccgat aggtgaccgt
     3121 cataccgagc ggctggcgtt cgtagtactc ggcgacacca tctcgaccca cgctgtacgg
     3181 tcgcagcccc tgaaagatcg cgtcctcggt gaacaccgcc gctaccgcct gcggatcatg
     3241 ggcgtcgatg cccgccttcc agtgctccag gacgccgcgt aggagctcag tgtcccgcac
     3301 tttgaccacc gtccacatgc aggatctcgc cggtcacgaa ggacgcgttc tccagataca
     3361 ggaccgcgtc gacgacgtcg tcgatctcac cgagacgacc gactggatgc agtttcgcca
     3421 gcgcctcgtg cgacgacgga tcgtgcatcg gggtgcggat caccccaagc gcgacggtgt
     3481 tcgcgcgaat accgcgcccg gcgtactcga tcgccagcga cttggtcacc gcgttcagtc
     3541 cgcccttggt cagcgacgcc aacgcagacg gcacctggga attggcgtgg tcgacgagac
     3601 tggtcgagac gttcacgatg tgaccgccac cttcgcgggt caacatcgcg gccacggcgg
     3661 cgcgcgaaac ctcaaagaac ccacggagat tcactcccat gatggcgtcg tagtcgtcgt
     3721 cggtgtattc cgtgaatggc ttggggatga agattcccgc gttgttgacc acggtgtcga
     3781 ctcggccgaa attttgcatg gccgcatcgg cgatgcgctg cccgacaccc ggctgcccga
     3841 tgtcaccagg caccgtcacg accatcgggt cgtcgctctc ggcgatggtg cgggagttgg
     3901 cgaccaccgc ataaccgagc ttgcgatagg ccttaatgag gcccttcccg atgccctgtg
     3961 aggcgccggt gatgatggcc acgcgttgcg aattgctcac tgtccgatcc ctttcttttg
     4021 tggatgcacg ctgcaacgcg ggctccgcgc cggccgatgc acggttgctg ctaccggttt
     4081 gctctctgtt gataggcgct gtctctcact gcactacgct tgttgggtgg agctgcgtca
     4141 gctgcgctac ttcgtgaccg tcgccgagga gctgaatttc ggtcgcgcgg ccgagcggct
     4201 gcacattgcg ggtccgtcgc tgtcccagca gatcaaggcg ctcgagcgcg acttgaagat
     4261 cagcctgttc gaccgtgacc gccgttccgt caccctgacc ccctccggtg cggctttgct
     4321 gccacacgcc cgcgcattga tcgatcaggc cgacgagctg cgcaggcgcg ctgccggtct
     4381 tcaggcgtcc gaacccgtca ggatcggcta cgtgaactgg tgtcccaccg attgggcaga
     4441 gcgggccgcc ggggtggcgc ggttacgcgt cgacacctgg gtcatgccgt cgcataccca
     4501 ggccgcccgg gtgtccgacg gaagcctcga cctcgcgatc tgctgggtgc agacaacgga
     4561 tctcgcgtcg ctgtccctcg atgctcacct gatcggggtg gatcggctct atgcggtgag
     4621 tgtcggctcg gacacttcgc caatcgaggc gaaggacaca ctggtgctcg tcgatgcgga
     4681 cgaggcgagc tggtcatcgt ggaatcgcta cgccgagcag ttcgccgccg acaccggagc
     4741 acagatcgtg cgggtcgacg acgggggagt gaccgggcag gagttcttcg agcacgtacg
     4801 tcgcctgcgc agaccggtgc tgaacaaccc gaaggggcag aacgtgcagg agcctagcgg
     4861 gctggtgcgg cggccggtgg tgcatccggc accgctgtgg acgtggtcgc tggtctggcg
     4921 ccgcgacgaa ggcagcgcgg cggttcgtgc agttatcgac ggattcaccc gcgacgcacc
     4981 agaattggat gccgacggct ggctgccggc tgcggatccg caccggccga cgggaacccg
     5041 aaccgaagca gaccggcgac catcgccgac agcaggacaa cagccgccgc caccaggaga
     5101 gcgagtgaaa agccgtggcg gagaagcggt gtcaccgcca ccggccccag gatctgaccg
     5161 acggagtatc cgctagtcag caaggccacg gcgccgggga agccgagcag tcgcccggcc
     5221 gccagcgcca tcgtgctgac gccgatgaat gtcgccccga agaggaccgc cccggtgaga
     5281 gccgcgaccg ggcctggcgc cagtgcgggc agcgcgatgc cgaatgcctg gaggagcagc
     5341 gctcccgcca gcagagtcgg atgcgaccac cgcgcgcaca gccacgccca cagtgccgcc
     5401 gatgggaccg cggccaaccc aacgacgagc catgcgccgc tgccgagcca gcccgacgaa
     5461 ttctgtttga tcgccgcgac caggaatgtg cccgcgacga tgtacccgat tccctccagc
     5521 gtgtagctga cgaacagcac cgcgaaccag cggttggcgc gtcgtggtga tgacggtggg
     5581 gcggccgcgg gacgggaggt cccgcgcatc gcccaagcgc ccgcgcccag caccgcggct
     5641 actgccgccg ccgtccacca cgcgccctgc caacccgcct ctgtcggcag cgtcagcacc
     5701 aacgcacccg agagcgcgat gcccatgccg attccgccga agccccagcc cggaagatgt
     5761 ggtggcagat gatcgagcat cgagttgacg gcgatgacga acgccaccgc actcgcgaat
     5821 cccgcgaccg tccgcaacaa cagccatcca attatgttgg gcgccaacgg cattgccgcg
     5881 agactgatta ccaacacggc caacgatgcc cgccacgcga cggtcgatcg ggccagccgt
     5941 ggcgaggcgg tgccggccag cgcgcccgcg aggtatccga cgtagttcgc ggtggccagc
     6001 gtccccgcgg cttgtggggt gagcacggcc tgcgtcgtca tcagcggcag gatcggggtg
     6061 taggcgaagc gaccgatgcc catggcggca cccaatcccg cggctccgcg gccgacgtgt
     6121 gcccagtgcg tgtgcatcgc ccaagcctgc gctgggcaga cgcacggatg ccacgaccga
     6181 attgctctca gctgggagct gctacctcac aacgggccat cgggcaaagt gaatgaacgg
     6241 taacgcctag ccatcgaaac ttaacggcca cgtgaacctg tggggtcgct gatgtatcaa
     6301 acctcgtgga gtatctctcc gtgctatcga tttcacgcga ggtcgacctc gaacctcacc
     6361 gcccggtgtg ggtgccgcga ccgcgcctcg tttctcaacg cgctgacggc tgggcgcggc
     6421 cggcgcatcc tgccgatatc gcgcaggcgc ggttgttcga cgccgctctg ttaggcgaga
     6481 tcgccgaact ggaggcgctt gtcgcctcga tggaaaagcg ttggctgcgg cggtgtgagc
     6541 gtggcatcga tgatgagaac cggccgccgg agaacttggt gcggatgcga ggccgcgtgt
     6601 ccgaagccca gcagctcctc gatgcacttc gggagcggtt cccctcgaag tgagcgaatc
     6661 cccgccctat gacggagatc cgctcaccga gattcaaact gacggggtcc agacaccggt
     6721 cgcggcggcc tcgcgggcgt agtcagcgaa gtccttgggc gcgcgtcgag tgcacgctgg
     6781 actccgtcgg ccagctccgc cttgctgccg agcacttcgg tgaacaagtg gttcaacaac
     6841 cagatgaagt cttgcggcag accgtattcc gtgagcatcg tggtgtagtc gtcgatcgga
     6901 accgatatga agctgatgtc ccggcctgcg gccgcgccga tctccgcgac cgcgtcgcgg
     6961 aaggtgagta gccgcggacc ggtcagctcg tagagctggc ccacatgccg gtcgtcggtc
     7021 agcgcggcca cgacgacgtc ggcgatgtca tcggcgtcca cgaacggttc ctgcacccga
     7081 tcgaccggca acgagatggt gccggccagc acctcatcga gccatgcgcc ctcgttgaag
     7141 ttctgcgcga agaacgcaca ccgcacgatc gtccactcca gtccggacgc catcacgacg
     7201 cactccgcct tctcggcttc tgcttcgttg cgtccggaca gcagcaccat ccggctgact
     7261 ccggcgtcct tggccatttg catgagcgtg ctgacgccgt cgactgagcc gggggcggct
     7321 aggtcggggt agtacgtgag gtagaccttg tcgacgccgg ccagcgcggc cgcccaggtc
     7381 tgcgggttgt cccagtcaaa cgggatatcc gccgagcgcg atccgtgccg cacagctatg
     7441 ccgcgctttt cgagttgatt gactacgcgc tttccggtct tgccggtgct gccgatgacg
     7501 agaatggtgt tggtggtcat gtcttgaatt caacggcccg gattcgagac gagccatggc
     7561 ccaaacgctc gatggcatgt ccaatcgtct ggaaggggat cggcatggaa aagatctcac
     7621 tcacggcgct cgcgcgccag cacctagagt cggcgcggac ggccagcagt gggcgcagcg
     7681 cacacacggt ctacggcggc catgaacaca gcctgcgtca gacgctgatg gcgatgacag
     7741 cgggtagcac gatggacgac cacgaaagcc caggcgaagc gacgcttcag gtgttacatg
     7801 gccgcgtccg tgtcaccaac tctcaatccg gctgggacgg ctcgcctggt gaccacatcg
     7861 tccttccccg cacccgccat ggcctacacg cattggagga ttcggtggtg ctactgaccg
     7921 tcgcgaaagc cgttggcccg cacgtgtagt cgcgcgtcga cggcctgctc tgtgtcgacc
     7981 gtcgtcacca tgtcgctctc gggtgggcta gtcggttgcc agcggggacc ggaggtcgtg
     8041 acgtcgatcg tgttcgacgg tgaaactcgc agcatgacga ccacggatgt cagctgtacg
     8101 aagcagcccg acggcggcct ggtgatcctg gtgcaggaca agcgaaggca tatggttcgg
     8161 ctccagctct cgcagcaggc gcgtcttgtt gttcacaagg cagggctgcg ctatgacgac
     8221 atggtcggtt tcgtggccga ccctcgggag gtcaccgcga ccaaggtcga cgacaccttt
     8281 accttcagcg ggcgcatgcc gccgaatccg ggggaatcgc agtggcacac cttcaaaatc
     8341 cagaccagct gccccggata tgtggatgcg ccgccgccca ggttggaccc agggatagga
     8401 gccccgtgaa cctcactaga cgtcgtgccg gtgactcagc acgttgacga cattgccggc
     8461 cgggtcggcg aagaagaacc gccgaacgcc ccactcctcg tcgctcagcg gatggacgat
     8521 ctgcaatccg gcgtcgaccg cggcctggta ggcggcgtcg acgtcgtcga cctcgatgga
     8581 aacgttcgga ttgaccggcg ctgtcgcgtc cttggtcatc aggctgattt ggtggcggag
     8641 ctcgtcaccg gccaacgtga cgacccagcc gaggttcatc acctcgcgta ggcccagtac
     8701 ccggatgtat gcctcacgtg ccgcgtccac gtcggagacc gtgaagatcg gcatcacccg
     8761 caggacggtc atatcgccgg ccccagcaga tcgtcggcgt ccttgatgac gtagccgtag
     8821 ccctgctcgg ccaggaaccg ctgccggtgc gcggcgtatt cggcgtccag gctgtcgcgc
     8881 gacaccaccg aatagaacat cgcccctccg ccgtcgtgct tcggccgcag cagccggccc
     8941 agccgctgcg cctcctcctg ccgcgacccg aacgtgcccg aaacctgtac cgccacattg
     9001 gcttccggca ggtctatgga gaagttcgcg accttcgaca ccaccaatgt ggacacctcg
     9061 ccgcggcgga acgcgtcaaa cagcgcctcg cgttcggcgg ttttcgtcga gccctggatc
     9121 accggggcgt tcagctcctg gccgagctcg tcgagctggt cgaggtacgc gccgatcacc
     9181 agtgtttggt caccctgatg cttttccagg atcgacttca ccaccgcgat cttggtgtgg
     9241 accgtagagc agagtttgta gcgctcgtcg ggttcggcga cggcgtagag catccgctcg
     9301 ttgtccgtca tcgtcacccg cacctcgatg cattcggcag gcgcgatcca gccctgggct
     9361 tcgatgtcct tccatggcgc gtcgtaccgc ttcggcccga tgaggctaaa cacatcgcct
     9421 tcgcggccgt cctcgcggat gagcgtcgcg gtcagcccga gccgccggcg cgactgcagg
     9481 tccgcggtca tccggaacac cggcgcgggc agcagatgca cctcgtcgta gatgatcaag
     9541 ccccagtcgc ggctgtcgaa cagttcgagg tggcggtatt cgcctttggt gcgacgggtg
     9601 atcacctggt aggtggcgat cgtgacgggc cggatctcct tcttctcgcc tgagtactcg
     9661 ccgatttcgt cttcggtcag cgatgtccgg ttgatgagtt cgcgcttcca ctgccggccc
     9721 gcgacggtat tggtgacgag gatcagcgtg gtcgcaccgg ccttggccat cgcggccgcg
     9781 ccgaccaacg tcttgcctgc gccgcacggc aacaccacca cgcccgagcc gccttcccag
     9841 aacgactccg cggccatctc ttgatagtcg cgcaagtgcc agtccgcttg atccagcgcg
     9901 atcggatgct tctctccgtc gacgtagccc gcgagatcct ctgcgggcca accgattttg
     9961 agcagcatct gcttgacgcg accgcgctcg ctgttgtgca ccatcacggt gtcgtcgtcg
    10021 atgcgcgcac cgagcatcgg ggcgatcttc ttgttgcgca gcacctcctc cagcacggcc
    10081 cggtccaggc ttaccagggt cagaccgtgt gccgggtgct tcaccagttg tagtcgtccg
    10141 tagcgggcca tcacgtcgac gatgtcgaca agcagtggct gcggcaccgc gtagcgggag
    10201 aaggacacca gcgcgtcgac aacctgttcg gcgtcgtggc ccgcggcccg cgcgttccac
    10261 agcgccaggg gagtgatgcg gtaggtgtgg atgtgttcgg gcgcgcgctc cagctcagcg
    10321 aacggggcga tcgcggcacg cgccgcgccc gctagctcat ggtcgacttc gagcagcacc
    10381 gtcttgtcgg actgcacgat cagggggccg tcggtcattt tgtccattat ccagggtcgg
    10441 cggacagcgg ccgtttcagt gcgcggcggt gggcattggc gcagctgacg ggcgtccacc
    10501 tgcgccgggc agcgggatct tgggtacttc gggtgtgccg atctggccgg ccagccacgg
    10561 aagcgagtcc ttgaagacgt ccatcgcaaa cggccaatcg tgtttacccg gggaggccac
    10621 gaccgcgcag gcaatgccgt tggcccgacc aagcgagcac agcgagttgg cggcatcgtt
    10681 ctggtcgccg gggttgcccg cggcgtcctg gccgccgagc ccggtcgcat tggggttagc
    10741 gtagccaccc ttgcggggtg tcggggcgtc ggaggagatc gcgaaccacc ccgaggcacc
    10801 ttggtacgga ccgtgtttgg tgatcaccgt ggtggggtcg tagatcgccc acttggcggc
    10861 gtcgccgccg tagatgcggg cgatcgtctg ctccttggtg ccggcattgg gccccatgtc
    10921 gccggcgatg tcgacgaatg tgctgaacag ctgcgggtgc atgacggtca ggtccaccgc
    10981 gcacgtgccg cccatcgacc agcccaccac accccagttg gcgttctgca cgccgaaggt
    11041 gcttgagacg tacggcggca cgtccttggt gaggtggtcg gccacgttgc cgcggggacc
    11101 gtcgacgcat tcggtgtcgt tgttgaacga cccgccgacg tcgacgaaga cgagcaccgg
    11161 cgcgttgccg ccgtgcgccg cggcgaaggt gtcgatggtg gcgatcgcgt tgccggtgcg
    11221 cggccagtcc gacggcgtgt tgaactcgcc ggcgatcatc atgacgagcg gcagctcggg
    11281 cgcattcgcg gcgaaccacg ccggtggcag atacacgaac tcggtgcggt gtttgaagcc
    11341 tgacgcgtcg gcgggaatcg tcacgggcac caacgctccg tgcgcgggaa tccccttgcc
    11401 ccgcatggcc attacggtgt tcaggtcggt ctcgtcggga agtgggcccg cggtcaactc
    11461 gttccacgcg gcctgaaccg tcgggaagta gccgacccac aggttcagcg caacccctgc
    11521 gctgagcacg cagagcggca ctgccaccag cgatacgatc cggcgccgcc accggctgct
    11581 gcgccaaccg agcaccgcga cggccaacgc gacaccggtg aggccaaccc aaaccaatag
    11641 cgacgacggc ggtgggtcgc ccgccaggcc ttccgaatcg acataccaat gcgcccatgc
    11701 cgccaggatc aagccgacca cgagcgccac cggcagccac accagccgcc accgtcgcga
    11761 ccgccactcg atcgccacga ccagcaccac cgcggcgacg gtccatatcg tcggtggcaa
    11821 ccacccgtgc aacagcgaca gcccgtgccg gtatccgccg ggggcctgcg cggcctcgct
    11881 taacagcgtt gtcacacaga catattcacc aacagtcctg tgaacttcct gccctgcggc
    11941 tggacggcct aggcggagtc cgccgacacc acggaggtga cccggtggat agcgaactcg
    12001 cggacccggc cggacgccgg atcgtaggcg gtcagctggc cgccgcggat attgatgggc
    12061 gccacgactc gctgggtggc cacgccggca gggtcgacgt aaccgatcac caccgacgac
    12121 tgcatatgcg cggcctcttg cagctgcgag atcgcaacgg ctggatcgag gcgcatgttg
    12181 ccgaacgacg cggacgccac cttgcgcagc accgcgacga tcgcggcgag cgtctgggtg
    12241 gtcggggtct gcggccagtg cacgcggcgc cgtcccgatg gcgcgggcac ccgggcgccg
    12301 cgggcgcgga tatcgacgat cgtgccggtg gaatcctcgg ccgcgggcgc gaaaccggcg
    12361 ccgcgcagcg cggccagcac ttcggagatc ggtgcctgcg acaccgcgac ggtcggggcg
    12421 agtagccgaa tctccagcag ctcgacggcg ggcgccgcga cggcctgcgc cagcaacgcc
    12481 ggatcctcgc accgcacgaa cgactgcgcc atgccgaccc gcagctgccc atgccggcgc
    12541 gcgacatcat cgatcagata cgtgagacct tgcggtacag gcgttttcga gtgcgtgctg
    12601 aacagcgtgt gcagctcact cgcggtgcgc ccggtgtcga gggcgcgccg gatcgacgcc
    12661 tcggtcaccc ggtacaccat cgccgcgccc gcggattcga ccgtcgccac cgacgccagc
    12721 ttctcggcga gatcgcgttg cagcggtccc ggcacgatga ccgtcaggtc ggcctgcagc
    12781 aggaagtggt cgatgggcgc gggcagcacc ttctccatcg cggcgacgac atcatcgggc
    12841 gggtcaccgg cgagcatgtt ccgcgcaggc gacgctatcg cgccgcggcc caccatgccc
    12901 agcgcgtggg cctctgtcag caggttgccg acgggttcgg gctgcaggcg caccgcccag
    12961 cggggccgcc gccagatcat cgcgcgtgac gcggaggtgg cgtcgacacc cgcacccggt
    13021 ggcagatcgg ccagcacgtc gagcagcaac cgccggtcca gtggggccgc cgtcgagaac
    13081 agcgaatccg acagcgccgc atagagtttg ccatccgggc cacgattgcc gaccaggctc
    13141 ggcctgcccg gcaggtccag ccacgtcgac gccagcaggt gccatttgga cgcggtcggc
    13201 gattcgacga accgatccgc agccactgtc ggcgcccagt acgggccgac gccgtcgtgc
    13261 gggtcgggtt ccggcatccc ggccgcgatc agtcccgccg ccgcggacac ttcgaggagc
    13321 aggccgagcc gggcctcttc gacgccggtg gtcttggcca acctcttgac gtcgcgcacc
    13381 cccagcccgc cgctgcgaag ctcgggaacc ggtgtggcgc cgagggtttc aagcaccagc
    13441 tcgacctcac gtagcaggtc gatcgccgcg ccggcagcca ccgcgtcgac gtcgcggacc
    13501 gccgtggtcg attccgtcgg atcgggttgc ctgagttgca ctggcccggg cagctcgccg
    13561 cgcagcacct gaccgaccag ccgcggcagg atcacggttt cgctgtcgac ctgacgcaac
    13621 aagcccgctg ccaacagccg ctgtaccggt cggtccggcg gagtacccgg tgcggcgtcg
    13681 cgggtccggc ccatcggtga gccctccagc agccgctgca gcagctcgag ttgcggcgcg
    13741 tcgagcgcct cgagccgggc ggcgatgtcc gtctgctcgc tgtcctcgac ggtggcctga
    13801 ccgggatacc agggcaagcc cgccgtggcc tcggcggcga cccgcacagt cgcgtcgccc
    13861 cagaccagcg cgcgctcccg caggtcgtcg acggcggcgg tgacggtgtc ctcgctcgcc
    13921 ttgtcgccga tcagcgccag cagcttggtc agcggcacgg ccgcggtgtc cgcatgcagc
    13981 acgagcagcg cgtcgaccac cgccagccgc agaaagtcga gtccgtcggt ggcggccttg
    14041 accgattgtc gggcctgcgc gcgggccgcc aacgccgcga tcgtgcccgg cggcggttgg
    14101 gtcaggtcgg gccgcagctc cagcagccgg atcagccgct cgtcgtcaag ctcggccagc
    14161 caggcgccga ggggaacgcc cggagtagtt tcggtcattg ctgaccagcg taaagcagcc
    14221 cgccggtgtc gccactggtc gcggcgcctg ccagaatgga tgcgtggctg acaagaaaca
    14281 gaaaaaccga tacgtcgacc cgggctggcc cgagctcgac ggcgacgacg atcacgcggt
    14341 cagtgaggcg gctaccgacc gcaccggcgc gctgtcgccc ttcggtgagc tggtgtttcc
    14401 gcagccggcc gacgagctgc cctatatcca cccgatcacg gtggtcaaca agtagcggtg
    14461 accgaccccg cgcgtcgcct ttcgcacctc gacgagtcgg gcgcggcgca tatggtcgac
    14521 gtcaccggta aggacgccac caagcgcacc gccgtcgccg ctggaacctt ccacacgacc
    14581 gccgatgttg tcgccctgat cgcatccggt gggctgccca agggtgacgc cgtggcaacc
    14641 gcccgggtgg cgggcatcct cgcggccaag cggaccagcg acctcgttcc gctctgccat
    14701 ccgctggcgc tcaccggcgt cgacgtcgac ttcgagatcg gcgatgacag catcggcatt
    14761 accgccaccg tgcgcaccac cgaccgcacc ggcgtcgaga tggaggcgct gaccgccgtc
    14821 agtgtggccg cgctgacgct ctacgacatg atcaaggccg tcgaccccgc cgcccacatc
    14881 gacgacatcc gggtgctgcg caaagagggc ggcaagaccg gcacgtggac acgatgacgc
    14941 ggtcaggccg ggtggtcatc gcttccacga gggcggcggc gggcgtctac gaagaccgca
    15001 ccggcccgat catcgtcgac tggctcaacg ggcagggcat cacgacgccg gctcccgtgg
    15061 tcgtcccgga cggctcgggt gtagtcaagg cgctgttcac cgcgatcggc gaaaacgtcg
    15121 acgtgataat cacttccggc ggcaccggca tctcgccgac ggacggcacg gccgacgcca
    15181 ctgcggagat cgtcgactac cagattcccg gcctcgccga tgcgatccgg cgctcgggtc
    15241 tgccgcatgt gccgacgtcg gtgctgtcgc gcggcgtgtg cggtgtcagg ggccgcacgc
    15301 tgatcgtgaa tcttccgggc tccactggag gcgtcaaaga cggcctcggc gtgctcgacg
    15361 acgtgctcga gcatgcgctc gatcaactca gcggcaagga ccacacccga tgaatgccgt
    15421 cgtgctacgc gtcgagctca ccgagcagcc gatcgagctg tccgagtacg aggcactggt
    15481 ggcccacgag tcggccggag ccatcgtcgg attcgccggt gtcgttcgcg atcacgacga
    15541 tggccgcgga gtgacccggc tagagtattc cgcgcacccc aaggcgcagc agactctcgc
    15601 ggacgtggcc gccgagatcg cggcggacag tcccggtgtg cgggccatcg cggtcagcca
    15661 ccgcatcggc acactgcaga tcggtgacgc agccttggtg gccgcggtcg ccgcggacca
    15721 tcgatcggcc gcattcgaga cgtgcgcgcg gctcgtcgac accgtcaagg cgcggctacc
    15781 ggtgtggaag caccagttct tcgccgacgg ctccgacgaa tgggtgaatt cggcctgacg
    15841 cgttaggcgg gcggcaggat cggcggcgcg tcgggcgccg gtggcgcggc cggatcggcg
    15901 ggaggtgccg gggtgaccgg cacgttcggg ccggggccct gctccgggta cggcgtgttc
    15961 atcccgcgtt gggccagccc catgatcaac gcttccttgc cgctgatgtc gtggttctgg
    16021 accgcttgcc acaggtcctt gaggtagctg acgttggggc cgtcggcgcc gttcgcggcc
    16081 ggatccatcg tggagcccgg cggcagggca tccgggctcg ccaggtgcgg gacgccgtcc
    16141 ggcagtgtcg gcagtgcggc ggcgggatcg gtcatcgcct ggttggcggc gtcaaacgcc
    16201 ggcgcgggaa cgttgacggc gttgagcggg gccagcggat ccggcgcggg cgccgctacg
    16261 gcccccggcg cgggcggcgc cgggggagcc ggtggggcgg gcgggagcgg cggcagtacg
    16321 gggtcgagcg gtgcaggcgc gtgcagcgcc cacacttccg gctgatcgac gggtgccggt
    16381 gcgacgtccc agttgtcggc ggccgcgacg atcgtgtctt cgatcggcgg agcatcgaca
    16441 gggggcggcg gaggcggcag cggcgcgtcc agcgaggcgt cgacgatctg tccctcgacc
    16501 ggcgcaggcg gcgggggcag cggtgcatcc agtggagcgg gagggggcgg cggcgcgtcg
    16561 ggcagcgggg ccgacagctc gtcgatcggt gcggcctcgg gcggcgccgg gggagcgaac
    16621 ggatctaacg gcggcggggg tggcagttcg ccgttgacag cgggggcgtc gagggcctgc
    16681 ggctcctcga tgacattgcg cggcgtcgca ccggacaggc ccctgccgca taccggccag
    16741 gcgcccttgc cctgggtggc aagaacccgc tcggcgaccg cgatctgctc ttctttggtg
    16801 gccatgtgcg cggacggcgc gtactcgccg ccgccgtgcg aagaccacgt actcggcgag
    16861 aactgcagcc cgccctgata gccgttgccg gtgttgatgg cccagttatt gcctgattcg
    16921 catttggcga cctgatccca ttcgccgtca gtggcggcgc cagcttgccc ggcgagggcg
    16981 aggcttccgc ttccgatgac agcgccggtg aaggcgattt ttgcgacgtt tacggccgat
    17041 gtagtgggct tgcgatgccg tccactcata ggtgcgctag gtcctctctt aagcgcccgc
    17101 gaggtcagct gtcgggttcg ggctggagag gtcgcccggc cgtgttgggc acggcttcac
    17161 cccaaggaac cgcaagcggt tccaagtcct gtgtattcgg tggaccggtg ggtcccccgc
    17221 ctccatccaa gttgttcgtc gttgtcccgt gcaccaacgg atggagctcg gcgcagttag
    17281 gcacggcggg ccattcgatt tgggtcgatg gcttgggagt gaacgctaac gggtcgccgt
    17341 ggtcacgtca tcttttgacg ctcgcggcgt ttccgaaggc agcaaatctt aaaaagggtc
    17401 taaatccgca ggacgccaac gtgtttccgc aggtgcgatt ttggaggtaa cggttcgtgg
    17461 tcatcttgtg accgtttcgt tatgtgacgt aaatcacggt tatccaccgg cgaacggggg
    17521 aagaacatcg acggtttgag cgtcgctcaa cgtcatcccc aagtcccgca ccgcaattcc
    17581 gtcacagaga tatgagcatc ggctcaggac tttcgccagt tcggtgtccc gttcacccag
    17641 gccttgcacc agcgcgccga cggtcgtgcc gggcgcgacg ccgatgatct cgtcatcgaa
    17701 gcccgccgcc gcccgagcgg ccgcgaagta gcggacagtc acctttaccg ccacgtcagc
    17761 cgccgatcgc actcatcggg cggtccggct gcacgaagcc cggatcgttg atgccgtggc
    17821 cagcggcctt cacccacatg gtggcgcgcc aggcggccgc cagtgcgtcg tcgtcggcgc
    17881 ccgcgcgcat cagccgccgc aggtcggtct cgtgctgggc gaacaggcag ttgcggatct
    17941 ggccgtcggc tgtgagccgg gtgcggtcgc atgccgcgca gaacgcgtgt gagaccgagg
    18001 cgatgatgcc gaccttaccg agaccgccgt tgacgtccca cagctccgcc ggcgccgaac
    18061 cgcggggcgc gcggtcgggt gtcagcgtga agtgccgttg caaggcgtcc aagacatcgt
    18121 cggctttgat cgcgcggccg cgtttccact cgtggccggc gtccagcggc atctgttcga
    18181 tgatgcgcag ctggtatccc tggtccaggc agaaccggag cagcgcgacc gcatcgtcca
    18241 gcccggtgac cggatccagc acggcgttca ccttcaccgg ggccaaccca gccgccttcg
    18301 cggcctcgag tcctcttagt acgtcgtgca gtcgatcgcg gcgggtgatg tgcgcaaagc
    18361 gcgctgcgtc gacggtgtcg agtgagacgt tgatgcggtc cagccctgcc tgcttgagtc
    18421 tggcggcacg cttgtcgagc ccaatcccgt tggtggtcaa cgtgatttcg ggacggggtc
    18481 gtaacccggc cgtggcggcg acgacgtcct cgaggtgctt ggccaccagc ggctcgccgc
    18541 cggtgaaccg gacgctggtg atgccgagcc ggatgaccgc gatgcgcagc agccgggtga
    18601 gctcgtcggt gcgcagcagc tgctcgccgg gcagccagtc cagcccttcg gcgggcatgc
    18661 agtatgtgca gcgcagat
//
LOCUS       DASRJA010000341         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_55027,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000341 DASRJA010000000
VERSION     DASRJA010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55027"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VFC01_15120"
     CDS             <1..979
                     /locus_tag="VFC01_15120"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN80995.1"
                     /translation="ARTPVLPSSERRSARHVSREARASFALAGAALFWSGNFIAGRAL
                     RNDIDPITLNLLRWALCLAIFLPWVGPKAWRCRSIVVREWRLLLGLGITGIAAFQTLV
                     YLALASTTAINALLILSLAPAAILIGASLTGRRRPSRMQWAGTLVSLAGACILVARGN
                     PALLLNLSPARGDLWMIVAVVLWAAYSLLLRRRPADLPQDVALAASIVPAVALLLALR
                     AIVSHAPVLPTPPLVGALIYVAIFASLFAFLLWSYGVSTIGPERAGQYVHLMPVFGTL
                     LAIEVLGERLALAQVLGALVVLAGIALVQRRSARPDADPPEERATRRII"
     gene            complement(972..>2020)
                     /locus_tag="VFC01_15125"
     CDS             complement(972..>2020)
                     /locus_tag="VFC01_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN80996.1"
                     /translation="YFDGGLSGAGYGITLDPKGNVWIGNFGFQDSPCEHLPIAVKSNS
                     VSVFRPDGSPVSGPGGFTQGGIFWPMATVSDRDGNIWVANCGTDSVTKIPKGNPDHAL
                     NIPLGPPPPAGDPQIKPFGVVVDGKGNVWVTGNRGNSVYVVSNDGETVTTLSGTDQGK
                     TVLTHPIGNALDSKGNVWVSNSDWLDAPCPTKNSLGTATNPSVTMFQGKDRTPFPGSP
                     FTGGGITLPWGIAVDGDDTVWVFNFGNTPPVSGPNAIPTAVSRFCGVDTRKCPPGLRT
                     GDPISPSTGYQSDSLQRITGGQIDPSGNVWLTNNWEIHGDPVQNPGANAVVIAIGAAA
                     PLKTPLIGPPLPFK"
BASE COUNT          295 a          659 c          672 g          394 t
ORIGIN      
        1 ggcccggacc ccagttctcc cttcgtccga acgccgatcc gcacgtcacg tttcgagaga
       61 agcccgcgct tcgttcgcgc tggccggtgc cgcgctgttt tggtcgggca acttcatcgc
      121 cggccgcgcg ctgcgcaatg acatcgatcc gatcacgctg aacctgctgc gttgggcgct
      181 gtgcctggcg atctttctgc catgggtcgg ccccaaggcc tggcgctgcc gaagcatcgt
      241 cgtgcgcgag tggcgcctgt tgctcggact cggcatcacc ggcatcgccg cattccagac
      301 gcttgtgtac ctggcactcg cgagcacgac ggcgatcaac gcattgctga tcctgtcgtt
      361 ggcacccgcg gcgatcctga tcggcgcgtc gctgaccggc cgccgtcgtc cgtcgcggat
      421 gcaatgggcg ggcacgctgg tgtcgctggc gggcgcatgc atcctggtcg ctcgcggcaa
      481 cccggcgctg ctgctcaacc tgtcccccgc gcggggcgat ctgtggatga tcgtcgcggt
      541 ggtgttgtgg gccgcctact cgttgctgct gcgccggcgg ccggcggatc tgccgcagga
      601 tgtcgcgctc gcggccagca tcgtgcccgc ggttgcgctg ctgctggcgc tcagagcgat
      661 cgtgagccac gcgcctgtgc tgccgacgcc gcccttggtc ggggcgctga tctacgtcgc
      721 gatcttcgcc tcgttgtttg ccttcctcct ttggtcctac ggcgtgagca cgatcggccc
      781 ggagcgcgcc ggacagtacg ttcacttaat gccggtgttc ggcaccttgc tggccatcga
      841 ggtgctcgga gaacgactcg cacttgcgca ggtcctgggc gcattggtgg tgttggccgg
      901 gatcgcgttg gtccagcgcc gttcggcgcg acccgatgcc gacccacccg aagaacgggc
      961 gacccgcaga attatttgaa cgggagcggg ggaccgatca gcggcgtctt gagcggcgcc
     1021 gccgcgccaa tcgcgatcac gaccgcattg gcgccgggat tctgaaccgg gtcgccgtgg
     1081 atctcccagt tgttggtcag ccagacgttg cccgagggat cgatctggcc accggtgatt
     1141 ctttgcagcg aatcgctctg gtaaccggtc gatggcgaga tcgggtcgcc ggtccgcaaa
     1201 ccgggcggac acttcctcgt gtcgaccccg cagaatcggc tgacggccgt cgggatggcg
     1261 ttggggccgc tgaccggcgg ggtgttgccg aagttgaaca cccacacggt gtcatcgccg
     1321 tcgacggcga tgccccatgg cagcgtgatg ccgccgccgg tgaacggaga tcccggaaac
     1381 ggcgtgcgat ccttgccttg aaacatggtc accgaaggat tggtggccgt gccgagcgag
     1441 ttcttcgtcg ggcagggcgc atcgagccag tcggaattcg agacccagac gttgcctttg
     1501 ctgtccaacg cgttgccgat cgggtgcgtg agcaccgtct tgccctgatc ggtgcccgac
     1561 aacgtcgtca ccgtctcgcc atcgttcgag acgacgtaga cgctgttgcc gcgattgccc
     1621 gtgacccaga cgttgccctt gccgtcgacg acgacaccga agggcttgat ctgcggatca
     1681 cctgcgggcg gcggtggccc gagcggaatg ttgagtgcgt ggtccggatt gcccttcgga
     1741 atcttggtca ccgagtcggt gccgcagttg gcgacccaga tattgccgtc gcgatccgat
     1801 accgtcgcca tcggccagaa tatgccgccc tgtgtaaaac ctcccgggcc tgataccggg
     1861 ctgccgtccg gacgaaacac cgacacgctg ttgctcttca ccgcgatggg caagtgttcg
     1921 caaggcgaat cctggaaacc gaaattgccg atccagacgt tgcctttcgg atcgagcgtg
     1981 atgccgtagc cggccccgct gaggccgcca tcaaaatagg
//
LOCUS       DASRJA010000342        12617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2967145,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000342 DASRJA010000000
VERSION     DASRJA010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12617
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2967145"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VFC01_15130"
     CDS             <1..401
                     /locus_tag="VFC01_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HZN80997.1"
                     /translation="RWLLRELVYALLVTKDHAAAGKIVVLMGAVGTVKPEAQAAKDLE
                     KFATPLTMSTLGDMSYADIGRQLSTLADAYDVKLPRELVLIGKQFLYVERYMKLLAPK
                     WQMMSDPQLTGYFANFMVEVSREHTEEVEA"
     gene            402..1304
                     /locus_tag="VFC01_15135"
     CDS             402..1304
                     /locus_tag="VFC01_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN80998.1"
                     /translation="MEVRTGTAKSGDLDIYYQDMGDPNNPAVLLVMGLGAQLLLWRTE
                     FCEKLVDQGLRVIRYDNRDVGLSSKITHRHSGMPLPTRMVRSFLGLPSPAVYTLEDVA
                     DDAAALLDHLKIDTAHIVGASMGGMIAQVFAARHKVRTKTLAIIFSSNNRPLLPPPGA
                     KQLLAILQKPKDATREGIVENAIRVTRITGSPGYPAPEERIRAEALEGYDRSYYPAGV
                     GRHFAAVLGSGSLLRYDRQISVPTVVIHGKADKLMRPFGGRAIARAIKGARLVLFDGM
                     GHDLPRELWDDIVGELKTTFAEAR"
     gene            1436..2323
                     /locus_tag="VFC01_15140"
     CDS             1436..2323
                     /locus_tag="VFC01_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HZN80999.1"
                     /translation="MAQAKKLQPHFEDVQAHYDLSDEFFRLWLDPTQTYSCAYFERDD
                     MTLEEAQLAKIDLALGKLGLQPGMTLLDVGCGWGSTMMRAIERYDVNVIGLTLSENQK
                     AHVEKVFAQSDSPRSKRVLLEGWEQFDEPVDRIVSIGAFEHFGFDRYDDFFKLAHTLL
                     PDDGVMLLHTITALTLPQMADRKIPLTFEVARFVKFILTEIFPGGRLPSIEKVGEHSA
                     TAGFELTRRQSLQLHYARTLDCWAEALQAHKDEATAVQSEEVYDRYMHYLTGCAKGFR
                     IGYIDVNQFTLGKVASSGA"
     gene            2334..3194
                     /locus_tag="VFC01_15145"
     CDS             2334..3194
                     /locus_tag="VFC01_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HZN81000.1"
                     /translation="MAELTPHFDDVQSHYDLSDDFYRLFLDPTQTYSCAYFERDDMTL
                     EEAQLAKIDLSLGKLGLQPGMTLLDIGCGWGATLRRAVERYDVNVIGLTLSQNQHDHA
                     EKAFATLDSPRSKRVLLEGWEQFAEPVDRIVSIGAFEHFGHDRWEPFFRTTYDALPRD
                     GVMLLHTITRLTLDEVKKRGIPISMDAARFAMFIAREIFPGGQLPTIGAVEEHATAAG
                     FTVARTQSLQAHYARTLDTWAQALDARRDEAVAVQSEEVYVRYMKYLTGCADMFRKGY
                     IDVNQFTLAK"
     gene            3312..4208
                     /locus_tag="VFC01_15150"
     CDS             3312..4208
                     /locus_tag="VFC01_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HZN81001.1"
                     /translation="MSDNSTGTKDMRPHFEDIQAHYDLSDDFFGVFQDPTRKYSCAYF
                     TGPNVTLSEAQIADVDLNLDKLDLKPGMTLLEVGCGWGLTLQRAMEKYDVNVIGLTLS
                     KNQKVYCDRLLANSNSNRTFDVRLEGWEQFHSPVDRIVSIEAFEHFGFERYDDYFKTC
                     FDILPDDGRMTIQSSVGYHPYDLAERGKKLTFELARFIKFMITEIFPGGRLPTTQMMI
                     EHGEKAGFVVPEAKSLRNHYIKTLGIWADRLEQNKDAAIAATDEENYHRYMRYLKGCQ
                     YYFIDESIDVSLVTYLKPGAAA"
     gene            complement(4290..5912)
                     /locus_tag="VFC01_15155"
     CDS             complement(4290..5912)
                     /locus_tag="VFC01_15155"
                     /inference="COORDINATES: protein motif:HMM:NF019842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HZN81002.1"
                     /translation="MRKIARSILLVVLALFSAVVLGVAAMLTSAGLSLAIVKPVTALI
                     MGGSGTPDPDLEPGYVPNVSSYYIFPNTECDSDDNCTLQAVVTPEEAWPLYGGLTALT
                     WQDSVNAGVEDLDAVVQPRLATLSDTNRLVIFGYSQSGAIASIEKQRLADQLSEEDQQ
                     WVEWVVTGNVSRPNGGLNVRLPITLPIVEFPYGPPTPTDTDMLTTDIALKWDIIADAP
                     LYPLNLLAMFNALVGGPGFGINHGTYPNPAGDPPTGLVGGYTQEEWQAIMDNPQGYAD
                     ANPDVVNVQTFGDTTYITVAPRVLPLLVPLHTIGLGFVADLIEPALRVIVEETGYNRS
                     IPYGQPTPFQLIPIFNPIQLAIDLIAAIPEGINQALNGGRPPLTPPETDTMLTTSTTL
                     SDSVDSLATVDVSKDGTTAPESGSTTSNGGGTTLQKTVVETSSGANATESNGTEVKQV
                     DPTKQDVTKGSDEIKTDSQGTKADPEGTKNDSEGTKKDRGETKQNGSGVSVSLNFSPK
                     KPKDGDTGGTTARHDAAGTTSPEGAESEQAAA"
     gene            complement(6012..7583)
                     /locus_tag="VFC01_15160"
     CDS             complement(6012..7583)
                     /locus_tag="VFC01_15160"
                     /inference="COORDINATES: protein motif:HMM:NF019842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HZN81003.1"
                     /translation="MRKSARTLAVVLAVLSSAVLGIVSAFMAAFAVGAATALIVPGTG
                     TPNANIVQNYMPHAADRYIAPFEPCSTADDCNLKGIDYPASFWPLGLIGNWCPGYQCD
                     KWDVSVGKGVENLDTALRTELAKPCPLTGCEPIVIFGYSQGGAVVSNGLRNIGDVTPD
                     QLGRLSVVMIGNAFNPDGGFFTRLGFLGHVPLIDITFGLATPVDTGIPMTAIGFQYDP
                     VMYAPLYWGNPFSLVNALAALETVHGYYLTPNGNGPTDPIAYGYTEAELAEQLDPTLH
                     PENFREDSEGNVYVMIPAKSLPIMDLITSVLPGPLQPVAKPVVDLVSPVLKVLIDLGY
                     DWSGDPGVERWLSPLPFNPIQNWPAVGVNLVAATVQGIQAFIGDLGGLTTTILPSTPP
                     PLSDVTTVSTLAAPAPSTPVDSPPAGTLKLVRDNTTAGPNVDVVKDKLSTGDTTTEAT
                     NETPPSTPNEPPVAKPAESKPDETTKVDDKKDAAEKKAGDTQEPDAKTDEEKKPDIGK
                     KPNSDTSASDQKAAA"
     gene            7739..8437
                     /locus_tag="VFC01_15165"
     CDS             7739..8437
                     /locus_tag="VFC01_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZN81004.1"
                     /translation="MHYFALLLGEPTSPLNPEEQAAGMAAYQAFHAKAASAIRAGDAL
                     TPSATGVRITGGPDASTVTDGPFAESAEVACGYYVFEAENLDEALALARDVPAAQHGA
                     VEVWPIVEYFPPSKPVGTSWLALLLEPAANAHAPGTPEWDKGIAEHQKFGAAAGERTN
                     GGAPLHPPTTATTVRVRDGEVLLTDGPFIEGAEVANGYYIIDADDRDEAIKIASMIPA
                     TAVELRQLMGVSGL"
     gene            8446..9669
                     /locus_tag="VFC01_15170"
     CDS             8446..9669
                     /locus_tag="VFC01_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN81005.1"
                     /translation="MANLDGVFRREWGPAVAALARWSGDFTVAEDAVQEAFSEALRAW
                     PRDGVPDNAAGWIVTVARNRALDRLRRESVRPGKELAAVVDDIRARTERVDVHPVRDD
                     ELRMMFTCAHPALDPTSQLALTLRLISGLTVAEIARALLQSEAAVGQRITRAKNKIRH
                     ANIPLRVPPAELLPERKPHVLACIYSVFTEGYWSTAGPSAIRDELCDEGVRLATELCS
                     LMPDERDAQALAALVLLHDSRRQTRVDADGTLVPLDEQDRSRWDRGRITRGLDRLQRA
                     AGARGPYLPQAVIAALHATAPSWQETDWATICAAYDRLIEQTGSPIARANQALAIGFR
                     DGFAAGLKALDEVAEDPRLARSNAVASIRADLLRRAGRRDEALRWYRIALDRNGSGPA
                     REFLRRRVEECVRYR"
     gene            9737..10060
                     /locus_tag="VFC01_15175"
     CDS             9737..10060
                     /locus_tag="VFC01_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81006.1"
                     /translation="MDADEDVAYYLAEWYLPELTETSVDEMVAKLDAAAILVSSEGAP
                     VRLVITLAVPTDEVLYGVFGAGSSDIVSKTCLQAGVPHQRLSGDVGTRIRRFPEPEVA
                     SQSAR"
     gene            10179..>12617
                     /locus_tag="VFC01_15180"
     CDS             10179..>12617
                     /locus_tag="VFC01_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN81007.1"
                     /translation="MPSVVNRPLELRAITEFLRSAAQQPSGLIIEGEPGIGKTTLWLS
                     AAEQARDSGYRVYSARVGQAESALAYAAVADLLRNIDASVLADLPEVQRVAVDRVLLR
                     ASSEDHATDQRAVAAAFTSAFERLAAESPVLIAIDDLQWLDRSSRDVVAFAARQFSGR
                     VGLLATERSDAEDTDASTWLQLSESDGIARVRVGPLSLGGLHVLISTRLGRSFPRPTM
                     VRIAEISGGNPFFALELAHSIDAQPSNARSRLPKSLSELMRLRIGQLDREARKLLLAT
                     ASAANPTVELVAQVSGAPAELLSDAEAKGIIAIDGDSVRFTHPLLAQSVYTDASPAER
                     RAMHRALAQTVTLPELRARHMALAASSADAETLRALDTAAQEAAARGAPAAAAELVEL
                     AIGLGGGLPERQIQAAEHHFNAGDPERARALLDETLGKLASGTLRGSALNLLAAVHLY
                     DDNWTEAVAVLERALDDAEDSPALLVQTLIVLAFGQRMISKHEESLVSARKAVAYAEH
                     LGDAALISRALAMSVQLNFMYGHGVDDVSLRRAVELEDPASEVLTPFSASMIEGLVLA
                     WTGQLDAAHARMEVLHRRCTERGAENDLTAVASCDALIEIWRGNFVAAAEQADEAVER
                     AEQGGGSLAIALSMRALVAAYLGREHDSRADAKAAVEIANRTVSPRLAEWPVMTIGFL
                     EVSVGNHAAALSAVQPMQRTFETIPGTEIMTASFIPDAVEAMIRLGRSGEAEPLIAAL
                     EGNGRQLDRPWMLAIGARCRSIWLAGQGDVDAAVRKAQEAMSEHDRLRMPFERARTQL
                     WLGQLQRRQRQKERS"
BASE COUNT         2287 a         4049 c         4062 g         2219 t
ORIGIN      
        1 cccggtggct cttacgagag ctggtgtacg cgctgctggt caccaaggat cacgccgcgg
       61 cgggcaagat cgtcgtgctg atgggcgcgg tcgggacggt caaacccgag gcacaggccg
      121 cgaaggatct ggagaagttc gccaccccgc tgaccatgtc gacgctgggc gacatgagct
      181 atgccgacat cggccgccag ctctcgacgc tggccgacgc ctacgacgtg aagctgccgc
      241 gggagctggt gctgataggc aagcagttcc tctacgtcga gcgctacatg aagctgttgg
      301 cgcccaagtg gcagatgatg tccgacccgc agctgaccgg atacttcgcc aacttcatgg
      361 tcgaggtcag ccgcgagcac accgaagaag tcgaggccta gatggaggtc cgtaccggca
      421 ccgccaagtc gggcgatctg gacatctatt accaggacat gggtgatccc aacaacccgg
      481 cggtcctgct cgtcatgggg cttggtgcgc aacttctgtt gtggcgcacg gagttctgcg
      541 agaagctcgt cgaccagggc ctgcgcgtca tccgctacga caaccgcgac gtcggtctgt
      601 cgtcgaagat cacgcaccga cactccggca tgccgctgcc cacgaggatg gtccgctcat
      661 ttctcggcct gcccagtccc gccgtctaca cgctggagga cgtcgccgat gacgccgccg
      721 cgctgctgga ccacctgaag atcgacaccg cgcacattgt cggcgcctcg atgggcggca
      781 tgatcgcgca ggtcttcgcg gcccgccata aggtccgcac caagacgctg gcgatcatct
      841 tctccagcaa caatcggccg ctgctgccgc caccgggggc caaacagctg cttgccatcc
      901 tgcagaagcc caaggacgcg acgcgcgaag gcatcgtcga gaacgcgatc cgggtcacga
      961 gaatcaccgg cagccccggt tatccggctc ccgaggagcg catccgggcc gaggcgctcg
     1021 agggctacga ccggtcctac taccccgccg gcgtgggccg ccatttcgcc gcggtcctcg
     1081 gcagcggcag cctgcttcgc tacgaccggc agatcagcgt ccctaccgtg gtgatccacg
     1141 gcaaggccga caagctgatg cggcccttcg gcggccgcgc catcgcccgc gccatcaagg
     1201 gcgcccggct ggtgctgttc gacggcatgg gacatgacct gccccgcgag ttgtgggacg
     1261 acattgtggg cgagctgaag accaccttcg ccgaagctcg gtaacatccc gcacatttct
     1321 cgattcgttg cccagggcgg aaggcgcgta gcctctgcag gagtgcttca gggcgtgtaa
     1381 gccgtcatcg tggacgctgg catcgctgct gaatagccag aaaggcacca gactcatggc
     1441 ccaagccaaa aaattgcagc cgcatttcga agacgtccag gcgcactacg acctgtccga
     1501 cgagttcttc cggctgtggc tggatcccac ccagacctac agctgcgcgt acttcgaacg
     1561 cgacgacatg accctggaag aggcccagct cgccaagatc gacctggcgc tgggcaagct
     1621 cggactccaa ccggggatga cgctgctcga cgtcggttgc ggctggggct cgacgatgat
     1681 gcgggcaatc gagcgctatg acgtcaatgt catcgggctg accctgagcg agaaccaaaa
     1741 ggcccacgtg gaaaaggtct tcgcgcaatc cgacagcccc cgcagcaagc gggtgctgct
     1801 cgagggctgg gagcaattcg acgagccggt ggaccggatc gtgtcgatcg gcgcgttcga
     1861 gcacttcgga ttcgaccgct acgacgactt cttcaagctg gcccacacgc tgctgcctga
     1921 cgacggcgtg atgttgctgc acacgatcac cgcgctgacg ctgccgcaga tggccgaccg
     1981 caagattccg ctcaccttcg aggtcgcacg gttcgtgaag ttcattctca ccgagatctt
     2041 ccccggcggc cggctgccgt cgatcgagaa ggtcggggag cattcggcca cggccggctt
     2101 cgagctcacc cgccgccagt cgctgcaact gcactacgcc aggaccctgg actgctgggc
     2161 cgaggcgctg caggcgcaca aggacgaggc caccgccgtc cagagcgaag aggtctacga
     2221 ccgctacatg cactacctca ccggctgcgc gaagggcttc cgcatcggct acatcgacgt
     2281 caaccaattc acgttgggga aagtagccag ttcgggcgcg tagcatgccc gggatggccg
     2341 agctcactcc gcatttcgac gatgtgcagt cgcactacga cttgtcggac gacttctacc
     2401 ggctgtttct cgatccgacg cagacctaca gctgcgccta cttcgaacgc gacgacatga
     2461 cgctcgaaga ggctcagctc gccaagatcg acctgtcgct cggcaagctc ggcctccaac
     2521 cgggaatgac cttgcttgac atcggatgcg gctggggcgc caccctgcgc cgggccgtcg
     2581 aacgctatga cgtcaatgtc atcggcctga ctctgtcgca gaatcagcat gaccacgccg
     2641 aaaaggcgtt cgcgaccctg gacagtccgc ggtccaagcg ggtgctgctc gaggggtggg
     2701 agcagttcgc agagccagtc gaccggatcg tctcgatcgg tgcgttcgag cacttcggcc
     2761 acgaccggtg ggaacccttc ttcaggacga cgtatgacgc gctgccccgt gacggcgtca
     2821 tgctgctgca cacgattacc cgactgacgc tggatgaggt caagaagcgc ggcataccga
     2881 tctcgatgga cgcagcacgg ttcgcgatgt tcatcgcgag ggagatcttt cccggtgggc
     2941 aactgccgac cattggggcg gtcgaggagc acgccaccgc agccggcttc actgtcgccc
     3001 gcacacagtc gctgcaggcg cactacgcgc ggacgctgga cacctgggcg caggcgctgg
     3061 atgccaggcg ggacgaggcc gtcgccgtcc aatcggagga ggtctacgtc aggtacatga
     3121 agtacctgac cggatgcgcg gacatgttcc gcaagggata catcgacgtc aaccagttca
     3181 cgctggccaa gtagcaccaa ttggagtaca gccgccgcta aacgctaggg tgtacggcta
     3241 gtcgcgcacg tcgcggccgt catggcacgt agagtgcggt ccacaccaga agaaggccaa
     3301 gcaggggaac tatgtctgac aactcaaccg gcacgaagga tatgcggccg cacttcgaag
     3361 atattcaggc ccattacgac ctgtcggacg acttcttcgg cgtgttccag gatcccacgc
     3421 gcaagtacag ctgcgcgtac ttcaccgggc ccaatgtcac gctgtcggag gcccagatcg
     3481 ccgatgtgga cctcaacctc gacaagcttg acctcaaacc cgggatgacg ctgctggagg
     3541 tcggctgcgg ctggggcctg accctgcagc gggcgatgga gaagtacgac gtcaatgtca
     3601 tcgggctgac gctgtcgaag aaccagaagg tctactgcga tcggctgctg gccaactcaa
     3661 acagcaaccg cacattcgat gtgcggctgg agggctggga gcagttccac agcccggtcg
     3721 accgcatcgt gtcgatcgag gcgttcgagc acttcggctt cgagcgctac gacgactact
     3781 tcaagacctg cttcgacatc ctgcccgacg acggccggat gaccatccag agcagcgtcg
     3841 gctaccaccc gtacgatctg gccgagcgcg gcaagaagct gaccttcgag ctggcccggt
     3901 tcatcaagtt catgatcacg gagatcttcc cgggcggccg gctgccgacc acccagatga
     3961 tgatcgagca cggcgagaag gccggcttcg tcgtacccga ggccaagtcg ctgcgcaacc
     4021 actacatcaa gacactcggc atctgggccg accgtctcga gcagaacaag gacgcggcga
     4081 tcgcggccac cgatgaggag aactaccacc gctacatgcg gtatttgaag ggctgccagt
     4141 actacttcat cgacgagagc atcgacgtca gcctggtcac gtatttgaag ccaggtgctg
     4201 ccgcatagtc cccagcttgc gcgtgggccc agcgccaaac ggaaatcccc cgccactgtc
     4261 taatgtgacg ggggattttg cttttccggt caggccgcag cctgttcgga ttccgctccc
     4321 tccggtgacg tggtgcccgc ggcgtcgtgg cgagccgtgg tgccgcccgt gtccccatcc
     4381 tttggcttct tcggcgaaaa gttcagcgaa acgctgacgc cggacccgtt ctgcttcgtc
     4441 tcgccgcggt ctttcttggt cccctcagaa tcattcttgg tcccctcggg gtccgccttg
     4501 gtcccctggg agtccgtctt gatctcgtcg gaacccttcg tcacgtcctg cttggtcgga
     4561 tcgacctgct tgacctccgt cccattggat tccgtcgcat tggcgccgga agatgtctcg
     4621 accacggtct tctgcagggt ggtcccgccc ccgttcgagg tcgtcgatcc agactccgga
     4681 gccgtcgtgc cgtccttcga cacatcaacc gttgccagcg aatccaccga gtcggacaac
     4741 gtcgttgacg ttgtcaacat cgtgtccgtc tcaggcggtg taagcggcgg gcgtccgccg
     4801 ttcagcgctt gattgattcc ctcaggaatc gccgcgatga gatcaatggc cagctggatc
     4861 gggttgaaga tcggaatcag ttggaacggg gtcggctggc cgtagggaat gcttcggttg
     4921 taaccggtct cttcaacgat gacgcgcagc gcgggttcta tcaggtctgc gacaaaaccc
     4981 agaccgatgg tatgcaacgg caccaagagt ggcagcaccc tcggggcgac ggtgatgtat
     5041 gtcgtatcgc cgaatgtctg cacattgaca acgtcagggt ttgcgtcggc gtacccctgc
     5101 gggttgtcca tgatcgcctg ccactcctcc tgggtgtatc cgcccaccaa ccctgtcggc
     5161 gggtcacctg ccggattggg atacgtgccg tggttgatcc cgaacccagg accgccgaca
     5221 agagcgttga acatcgccaa taggttgagc ggatacagcg gggcgtccgc gatgatgtcc
     5281 cacttcaagg cgatgtcggt ggtgagcatg tcggtgtcgg ttggtgtggg tgggccgtag
     5341 ggaaactcga cgatcggcag tgtgatcggc agccggacgt tgagcccacc gttgggccgc
     5401 gagacgttgc ccgtgacgac ccattcgacc cactgctggt cctcctccga cagctggtcg
     5461 gcgaggcgtt gcttctcgat ggaggcgatc gcgccgctct gtgagtagcc gaatatcact
     5521 aggcggttgg tgtcgcttaa tgtcgctagt cgcggctgca ccacggcgtc gagatcctcg
     5581 actcccgcgt ttaccgaatc ttgccacgtg agagcggtca gcccgccgta aagcggccag
     5641 gcctcttcgg gggtaacgac cgcctggagc gtgcagttgt cgtctgaatc gcattccgta
     5701 ttgggaaaga tgtagtagct cgagacgttc ggcacgtagc ccggctctag gtcaggatct
     5761 ggcgtgccgg agccgcccat gatcagcgcc gtcaccggct tcacaatggc gagcgataag
     5821 cccgccgacg tgagcatcgc cgccacacca aggacgacgg cgctgaacag ggccagcacc
     5881 acaagaagaa tcgaccgggc tattttgcgc atgtgtcaac gatcacacga cacggttacc
     5941 tcagtaggcg gaacggccca attaccgtct gtcgcattgt cgcccgctaa cgcaccgagg
     6001 ctcgtcttgg ttcaggccgc tgccttctga tctgaggcac tcgtgtcgct gttcggcttc
     6061 ttgccgatgt ctggcttctt ctcttcatcc gttttggcat cgggttcctg ggtgtcaccg
     6121 gccttcttct cggccgcatc cttcttgtcg tcgaccttgg tcgtctcgtc cggcttcgat
     6181 tccgcgggct tggccacagg cggttcgttc ggcgtcgacg gcggcgtctc gtttgtagct
     6241 tcggtcgtgg tatcgccggt tgacagcttg tccttgacga cgtcgacgtt gggcccggcg
     6301 gtggtgttgt cccgcaccaa cttcagggtg cccgctggcg gtgagtcgac cggtgtcgat
     6361 ggcgccggcg cggccaatgt ggacactgtc gtcacgtcgg agagcggcgg cggagtcgac
     6421 ggtaggatcg tcgtcgtcag cccgccgagg tcaccgatga aggcctggat gccctgtacg
     6481 gtcgccgcga cgaggttgac gcccactgcg ggccagttct ggatcgggtt gaagggcaac
     6541 ggagacagcc acctctccac accggggtcg ccgctccagt catagccgag atcgataagc
     6601 accttcaata ccggtgaaac caggtcgacg accggcttgg ccaccggctg gagcgggcca
     6661 ggcagtaccg acgtgattaa gtccatgatc ggcagactct tggccgggat catgacgtag
     6721 acgttgcctt cggaatcctc gcgaaagttc tccgggtgca aagtcgggtc cagctgttcg
     6781 gcaagttccg cctcggtgta tccgtacgcg atcggatctg tgggaccgtt gccattcggc
     6841 gtcaggtagt aaccgtggac cgtctccaac gcggcaagcg catttactaa tgagaacggg
     6901 tttccccaat acagcggggc gtacatgacg gggtcgtact ggaagccgat cgcggtcatc
     6961 ggtatgccgg tatcgacagg tgtagccaac ccgaacgtga tgtcgatcag cgggacgtgg
     7021 cctaagaatc caagcctggt gaagaacccg ccatccgggt tgaacgcgtt tccgatcatc
     7081 accaccgaca atcggcccag ctggtccgga gtgacgtcgc cgatgtttcg caacccgttg
     7141 gatacgacgg cgccgccctg ggagtagccg aagatcacga tgggctcaca accagtcaga
     7201 gggcatggtt tagcgagctc ggtcctcagc gctgtgtcga ggttctcgac gcccttgccc
     7261 accgagacgt cccacttgtc gcactggtat ccgggacacc aattaccgat aaggcccaga
     7321 ggccagaacg acgcggggta gtcgatccct ttcagattgc agtcgtcggc ggtcgagcac
     7381 ggctcgaacg gcgcgatata gcggtcggcc gcgtgtggca tgtagttctg gacgatgttg
     7441 gcgtttggcg tcccggtgcc gggcacgatc agcgccgtgg cggcccccac cgcgaatgcg
     7501 gccatgaacg ccgacacgat gccgaggacg gccgacgaaa gcactgcaag gacaacagcc
     7561 agagtgcgcg ccgacttacg catggatgaa cgatcacaca atgccgacgc gccggtcgtg
     7621 gaacacggaa gtcgcttccg caaagttcgc tgaagtgtgt cggattcgcg cgacgccgtt
     7681 cgtcggagga gtagagagtg gaacacaagg ttccgctcgc gaccggaagg tgacccctat
     7741 gcactacttc gcgctgctgc tcggcgagcc cacctcgccg ctgaaccccg aggaacaggc
     7801 cgcaggcatg gccgcatatc aggccttcca cgccaaggcc gcgtcggcca tccgtgccgg
     7861 cgacgcgctc actccgtcgg cgaccggcgt gcggatcacc ggtggccccg acgcgtcgac
     7921 ggtgaccgac ggcccgttcg cagagagcgc cgaggtggcc tgtggctact acgtgttcga
     7981 ggccgagaac ctcgacgaag cactggcgtt ggcgcgcgac gtccccgcgg cgcagcacgg
     8041 cgccgtcgag gtgtggccga tcgtcgagta cttcccgccg agcaagcccg tcggcaccag
     8101 ctggctggcc ctgctgctcg agccggcggc gaatgcgcac gcaccaggaa cgccggaatg
     8161 ggacaagggc atcgccgagc accaaaagtt cggtgccgcc gcgggagagc gcaccaacgg
     8221 cggtgcaccc ctgcatccgc ctacgacggc gacgacggtg cgggtgcgtg acggcgaggt
     8281 gctgctgacc gacggtccgt tcatcgaagg cgccgaggtg gccaacggct actacatcat
     8341 cgacgcggac gaccgcgacg aggcgatcaa gatcgcgtcg atgattccgg cgacggcggt
     8401 ggagctgcgg caacttatgg gcgtctcggg gttgtaaccg cttcaatggc caacctggac
     8461 ggcgtctttc ggcgcgaatg gggtccggct gtcgcggcgc tcgcccgctg gtcgggtgac
     8521 ttcaccgtcg ccgaagacgc cgtccaggag gccttctccg aagcgctgcg tgcgtggccg
     8581 cgcgacggcg tgcccgacaa cgcggccgga tggatcgtga cggtcgcccg caaccgggcg
     8641 cttgatcggc tgcgacgcga atccgtccgc ccgggaaagg aactcgcagc cgtcgtggac
     8701 gacatccgag cccgcactga gcgcgtggac gtgcatcccg ttcgcgatga cgagctacgg
     8761 atgatgttca cctgcgcgca ccccgcgctc gacccgacct cacagctggc gctgaccctg
     8821 cggctgatct cgggcctcac cgtcgccgag atcgcccggg cactgttgca gtccgaagcg
     8881 gccgtcggtc aacgaatcac tagggccaag aacaagatcc ggcacgccaa catcccactc
     8941 cgggtaccgc ccgccgaatt gctgccggaa cgcaaaccac acgtgctggc ctgtatctat
     9001 tcggtgttca ccgagggcta ctggtcgacg gcagggccgt cggccatccg cgacgagctc
     9061 tgcgatgagg gcgtgcgcct ggccaccgaa ctgtgctcgt tgatgccgga cgaacgcgat
     9121 gcacaggcgc tggccgccct tgtcctgctt catgattcgc gacggcagac ccgagtggac
     9181 gccgacggga cgctggtccc gctcgacgaa caggatcgca gccgatggga tcgcggccgc
     9241 atcacgcgag gtctggatcg gctccagcgg gccgcggggg cgcggggtcc ctatttgcca
     9301 caagcggtga tcgcggcgct acacgcgacg gcgccgagct ggcaggagac cgactgggca
     9361 acgatctgcg cggcctacga ccggctgatc gagcagactg gctcccccat cgctcgggcc
     9421 aaccaggcgc tggcgatcgg cttccgagac gggtttgcgg ccggcctgaa ggcgctcgac
     9481 gaggtggccg aagacccccg actggcccgg tcgaacgcgg tggcctcgat ccgcgcggat
     9541 ctgctacggc gcgcgggcag gcgtgacgag gccctccgct ggtatcgcat cgcgctggac
     9601 cgcaatggct cgggcccggc acgcgagttc ctgcggcggc gcgtcgagga gtgtgtccgg
     9661 taccgctaag tcacgatgcc aagctgtagg gaaacacccg attccaccgc agcggcttaa
     9721 ggctaccttc gacgtcatgg acgccgatga ggacgtggcg tactacctcg ccgaatggta
     9781 tctgcccgag ctgaccgaga catcggtcga cgagatggtg gccaagctcg acgccgccgc
     9841 cattctcgtc agcagcgagg gcgcaccggt gcgcctcgtc atcacccttg ccgtcccgac
     9901 tgacgaggtg ttgtacggag tcttcggcgc cggttcttcg gacatagtgt cgaaaacatg
     9961 tctgcaggcg ggtgtgccgc accagcggct ttccggcgat gtcggcacac gaattcggcg
    10021 attccccgaa cctgaggttg ccagccagag cgcgcgttag ctcgctgcac tgtgtttaaa
    10081 acaacggttt agctgcccag caccgtcccg gaatccgcgc gaccagatgc cgtccgatgt
    10141 agtctcggtt tgctgtctga gatcggtggg ggttatgaat gccatccgtg gtgaaccgcc
    10201 cgctcgaatt gcgggcgatt accgagttct tgcggtcagc agcacagcag ccgtccgggc
    10261 tgatcatcga gggcgagccc ggcatcggca agaccacact gtggctgtcc gctgcggagc
    10321 aagcacgcga cagcgggtac cgggtgtact ccgctcgagt ggggcaggcg gaatcggcgc
    10381 tcgcctatgc ggcggtggca gacctcctcc gcaacattga cgcaagcgtc ctcgccgacc
    10441 tgcccgaggt acagcgcgtg gctgtcgacc gagtgctgct gcgagccagt agtgaggacc
    10501 acgctacgga tcaacgcgct gtggcggcag ccttcacgtc tgcattcgag cgcctcgccg
    10561 cagagtcgcc cgtcctgatc gccatcgacg atctgcagtg gctagaccgg tccagcagag
    10621 acgtggtggc gttcgccgcc cgacagttca gcggccgggt ggggctgctc gccaccgagc
    10681 ggtcggatgc agaagacacg gacgcatcga cgtggcttca gctgtccgaa tccgatggca
    10741 ttgcgcgcgt gcgtgtcggc ccgctgagcc tgggcggcct gcacgtgctc atctccacgc
    10801 ggctagggcg atcgtttccg cggccgacga tggtgcgaat cgccgagatc tcaggcggga
    10861 acccgttctt cgcactcgag ttggcacact ctatcgatgc gcagccttcg aatgcgcggt
    10921 cccgtcttcc gaagtcgcta tcggaattga tgagactgcg gatcggacag ctcgaccgcg
    10981 aagcccgaaa actgttgttg gcgaccgcat ctgccgccaa ccccaccgtg gaactcgtcg
    11041 ctcaggtctc cggtgccccc gccgagttgc tcagtgacgc agaagccaag ggcatcatcg
    11101 cgattgacgg cgactccgtg cgattcacgc atcccctgct ggcgcaaagt gtctacaccg
    11161 atgccagccc ggccgagcgc cgcgccatgc accgggcgct ggcacagacc gtcacactgc
    11221 ctgaactgcg ggccagacac atggcgcttg ccgcctccag tgcggacgcc gaaaccttgc
    11281 gtgccctgga caccgcggcc caggaggcgg cggcccgcgg cgcgcccgcg gcagcagccg
    11341 aattggtgga actcgccatc ggcctgggtg gcggcctacc ggaacggcaa atccaagcgg
    11401 ccgaacacca tttcaatgcc ggcgaccccg aacgagcccg tgcgttactc gacgaaaccc
    11461 tgggcaagct ggcatctggg acgctgcgcg gatccgcgct caacttgctt gccgcagtcc
    11521 acctctacga cgacaactgg accgaagccg tcgctgttct ggagcgcgcg ctggacgatg
    11581 ccgaggacag cccggccttg ctcgtgcaga ccctgatcgt gctggccttc ggccaacgca
    11641 tgatctcgaa acacgaggag tccttggtca gtgcacgcaa ggcggtggcc tatgcagagc
    11701 atctcggcga tgcggccttg atcagcaggg cgctcgcgat gtcggtgcag ttgaacttca
    11761 tgtacggaca cggggtggac gacgtcagcc tgcgacgagc ggtggagttg gaagatcccg
    11821 ccagtgaggt gctaaccccc tttagcgcca gcatgatcga gggtttggtg ctggcatgga
    11881 caggacagtt agatgccgcc cacgccagaa tggaggtgct ccaccgccga tgcactgagc
    11941 gcggcgccga gaacgatctc accgccgtag ccagctgcga cgcgttgatc gagatctggc
    12001 gaggcaactt cgtcgcagca gcagaacagg ccgatgaagc cgtggagcgc gccgagcagg
    12061 gtggcggttc gttggcgatc gccctatcca tgcgggcgtt ggtggctgct taccttgggc
    12121 gagaacacga ttcgcgcgcc gacgccaagg ccgcagtgga aatcgcgaac cggactgttt
    12181 caccgcggct ggctgagtgg cccgtaatga ccatcggatt tctcgaggtc tcggtcggaa
    12241 accatgccgc cgcgctgtca gcggtacagc cgatgcagcg cacattcgag acgatacccg
    12301 gcactgagat tatgaccgcg tcgttcatcc ctgacgccgt cgaggccatg atccgcttgg
    12361 gccgcagcgg cgaggccgag ccattgatcg cggcgctgga gggcaacggc cgccagctgg
    12421 accgcccatg gatgctggcc atcggtgccc gctgccgcag catttggctg gccgggcagg
    12481 gtgacgtcga cgccgccgtc cgcaaagcgc aagaggcgat gtcagaacac gatcgactgc
    12541 ggatgccgtt cgaacgtgct cgcacacaat tgtggctggg tcagctgcaa cgtcgtcaac
    12601 gccaaaagga acgctcg
//
LOCUS       DASRJA010000343        11255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_587036,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000343 DASRJA010000000
VERSION     DASRJA010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11255
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587036"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1831
                     /gene="glgX"
                     /locus_tag="VFC01_15185"
     CDS             <1..1831
                     /gene="glgX"
                     /locus_tag="VFC01_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HZN81008.1"
                     /translation="FHGDFDFSQALFSYDLNADDLASGGTPPGIDSLGHTMTSVVINP
                     FFQWASDRAPRTPYHETIIYEAHVKGMTQTHPGIPEEQRGTYAGLGHPVVIDHLKSLN
                     VTAIELMPVHQFLHDHRLLDLGLRNYWGYNTFGFLAPHYQYAANKHAGGAVAEFKTMV
                     RSFHEAGIEVILDVVYNHTAEGNHLGPTINFRGIDNAAYYRLQDGDLRVYKDFTGTGN
                     SLNPRHPHTLQLIMDSLRYWVLEMHVDGFRFDLAAALAREFYDVDRLSAFFDLVQQDP
                     VVSQVKLIAEPWDVGEGGYQVGNFPGLWTEWNGKYRDTVRDYWRGEPATLGEFASRLT
                     GSSDLYEATGRRPGASINFVTCHDGFPLADLVSYNEKHNEANGEDNRDGESHNRSWNC
                     GVEGPTDDPDILALRAKQIRNIMGTLMVSQGTPMISHGDEIGRTQRGNNNVYCQDSEI
                     SWMDWSLCEKNPDLLEFTRRVTKLRRNHPVFRRRRFFEGEPIRTGDQVRDIAWLTPAG
                     KEMAREDWGTGLGKCVAVFLNGEAIPTSNARGERVVDDSFLLCFNANDKPQDFVTPKG
                     DYAKEWTATLDTADATGSTELVVTAGKKISLQARSLLVLRKTA"
     gene            1835..4048
                     /gene="treY"
                     /locus_tag="VFC01_15190"
     CDS             1835..4048
                     /gene="treY"
                     /locus_tag="VFC01_15190"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HZN81009.1"
                     /translation="MVFPVLSTYRLQMRGDCFTFDDAVEELDYLDNLGVSHLYLSPIL
                     TAAQGSTHGYDVTDPTTVSAALGGPDGLARLSAAAKARGMGLIVDIVPNHVGVDNPEQ
                     NAWWLDVLAHGRSSPYAAYFDIDWDLDPESRIILPVLGSDGDRPPPGYPVDQSHYRPI
                     GWRNGICGYRRFFSITSLAGLRQEDRAVFDATHVEVKRWIAEGLVDGLRIDHPDGLSN
                     PAQYLAWLRELVGPDAWIVIEKILAVDEPLEVTLPVAGTTGYDALREIGGVLVDPAGA
                     GALTALFESSGVAYDEMPQLARGLKAAAVTDTLGSELARLHRTIVAEAGADHPDLPRA
                     VAELLSRVHVYRSDYLSLSLVLPEAVAETVSALPELAEPLAIIATAVTSSRESAVRLQ
                     QLCGAATAKSMEDCLFYRDARLVSLNEVGGEPARFGVSVAEFHERAATRSSLWPHTMI
                     ALTTHDTKRGEDVRARIGVLSQVPSLWAELVGKWALEAPPPDPATGLFLLQNIFGVWP
                     ADGVVTDELRGRVHTYAEKAIREAAVHTSWHDPDPAFEAAVHAWLDAVMDGPVAAQLT
                     SLVTRLDVYARSDSLSQKLLALTVPGVPDVYQGTELWEDSLVDPDNRRPVDYGVRREA
                     LKIMTHPKVRVVAAALRLRRQSSASFTDAGYTPILAEGPAAEHLVAFLRGADVLTTVT
                     RHPVRLSEIGWGDTALDLPSGTWVDRIGGRQYSGRVLAAELSAELPVALLERIDG"
     gene            4041..5771
                     /gene="treZ"
                     /locus_tag="VFC01_15195"
     CDS             4041..5771
                     /gene="treZ"
                     /locus_tag="VFC01_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HZN81010.1"
                     /translation="MVEFAVWAPRPERVRLDVDGTLYPMDRSDDGWWRTDVGAQPDAR
                     YGFVLDDDPKVLPDPRSPRQPDGVHERAQRWQPAADTWTDADWEGRSIEGRVIYELHV
                     GTFTPGGTFDAAVEKLDYLVDLGIDFVELMPVNAFGGTHGWGYDGVLWYAVHEPYGGP
                     DGLVRLINACHRRGLGVLIDAVFNHLGPSGNYLPHFGPYLSSGSNPWGESINLCDADA
                     DAVRRYIIDCALRWMREFHADGLRLDAVHALVDTTAIHILEELAAETDSLAEELGRPL
                     SLIAESDLNDPRLITPRDRGGYGMTAQWDDDIHHAIHSAVSGERQGYYADFGSLETLA
                     QTLKHGYFHAGRYSSFRHRRHGRPLDTASIPATRLLAYTMTHDQVGNRAVGDRPSQNL
                     TFGQLAIKAALALGSPYTGMLFMGEEWGSSSPFQFFSSHPEPELARATAEGRKREFAE
                     HGWDADEIPDPQDPATFERSKLNWDEIDDGEHGQLRRVYRQLIALRHNEPDLADPWLD
                     HMRVDYDEDGRWIVMYRGSLAIACNLGTDVVDVPVTGELVLTWGEPKVDTETTRLEGH
                     SFAVLRRVSG"
     gene            complement(5818..7098)
                     /gene="ilvA"
                     /locus_tag="VFC01_15200"
     CDS             complement(5818..7098)
                     /gene="ilvA"
                     /locus_tag="VFC01_15200"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HZN81011.1"
                     /translation="MSAELSQSPRTSPLSAADIDEAAQRISGVVLRSPLQFSERLSEA
                     TGATVYLKREDQQAVRSYKLRGAHNLLMQLSDEEIAAGVVCSSAGNHAQGFAMACRSM
                     GIHGRVYVPAKTPKQKRDRIRYHGREFIELIAIGKTYDEAAAAALDDVALTGATLVPP
                     YDDPRTMAGQGTIAAEILDQLEGEPDLVIVPVGGGGCIAGITTYLAERTTNTSVLGVE
                     PAGAAAMIAALAAGEPVTLEHVDQFVDGAAVNRAGTLTYGALAAAGDMVSITTVDEGA
                     VCTAMLDLYQNEGIIAEPAGALSVAALMEADIDPESTVVCLISGGNNDVSRYNEVLER
                     SLVHLGLKHYFLVDFPQEPGALRRFLDTVLGPNDDITLFEYVKRNNRDTGEALVGIEL
                     GSAADLEGLLERMKASDIHVETLEPGSPAYRYLL"
     gene            complement(7128..7565)
                     /locus_tag="VFC01_15205"
     CDS             complement(7128..7565)
                     /locus_tag="VFC01_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN81012.1"
                     /translation="MPLSGEYEPSPWDWVRKDADRITESGSTEGIEMKGKPLILLTTI
                     GAKTGKVRKTPLMRVEHDGQYAVVASLGGAPKNPVWYYNIKAHPRVELQDGDLNKDYE
                     AREVFDDEKAVWWERAVAAWPDYAEYQTKTDRQIPVFVLTPIN"
     gene            7654..7851
                     /locus_tag="VFC01_15210"
     CDS             7654..7851
                     /locus_tag="VFC01_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81013.1"
                     /translation="MTARDEVSIAGVAWPVYKLLALAIGFLVLVIVAVATTSAAPSVL
                     AAAAAGAVVWLALSPFHPSDR"
     gene            complement(7848..8249)
                     /locus_tag="VFC01_15215"
     CDS             complement(7848..8249)
                     /locus_tag="VFC01_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN81014.1"
                     /translation="MESRHVSIWIETDPEAVYEFAADPQAWPKWAAGLAEGGLRRTAD
                     GWVADSPMGQVTVEFAPPNEFGVLDHVVRLPSGEAVYNPLRVIPGGVGEACCEVVFSV
                     RRRAGMTDEEFDADGAAVAADLETLRTLLEN"
     gene            8363..9025
                     /locus_tag="VFC01_15220"
     CDS             8363..9025
                     /locus_tag="VFC01_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81015.1"
                     /translation="MPSWLRPGDPESRLPVLFALVAVIGLQLAIPKSYTLVPRWPLIG
                     LELLLLAVLTWLNPLKLTNATRLGRYATWVLLAAITLDNTLSAVLLDVRILTGEVSNK
                     AAVLLGSGGAIFVTNIIVFGIWYWELDRGGPFARRAGEKPYPDFMFPQMDPDTAKLVK
                     PGWRPTFVDYLYVSLTNAMAFSPTDTMPLARWAKGMMAVQSLVAVTTVALVIARAVNV
                     LG"
     gene            complement(9022..9750)
                     /locus_tag="VFC01_15225"
     CDS             complement(9022..9750)
                     /locus_tag="VFC01_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81016.1"
                     /translation="MADELKVVSDTKSNAGGHLVTEDYDKHPRACRHGVWIGWKETFS
                     QTVTLAFEHTGHPYVGWLINGIRVIDPGYGPFTQLPGDPCPGAPSVLYSCPVGGLFHQ
                     ISFTSVAGAPQACFSVQAIYREHGQQYLPPRDGPTTSLCLSGSDIEWPAFLILQEQAC
                     LRRWKDILRRYVEVADVGPLDPVALLSSLPDEERTILQAAAEALEEIKPDQQPKLSNA
                     LRDTVVGILRSRMPVPEDRARADG"
     gene            complement(9867..>11255)
                     /locus_tag="VFC01_15230"
     CDS             complement(9867..>11255)
                     /locus_tag="VFC01_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HZN81017.1"
                     /translation="IAQKVASYSLARADILRKAMGKKKREVLDKEYEGFSDGMKANGF
                     SAAAIKALWDTVLPFADYAFNKSHAAGYGLVSYWTAYLKANYPAEYMAGLLTSVGDDK
                     DKAAVYLADCRKLGITVLPPDVNESGLNFASVGEDIRYGLGAVRNVGANVVASLISTR
                     TDKGKYIDFSDYLNKIEIGPCNKKVTESLIKSGAFDSLDHPRKGLFLIHTDAVDSVLG
                     TKKAEAMGQFDLFGGADTATDAVFTIKVPDEEWEDKHKLALEREMLGLYVSGHPLNGI
                     AHLLTAQVDTQIPAILDGDVANDTQVRVGGILASVNRRVNKNGLPWASAQLEDLTGGI
                     EVLFFPQTYSTFGAEIADDAVVLVGAKVAIRDDRISLIANELVVPDFSSAQVNRPLAV
                     SLPTRQCTVDKVTALKQVLARHPGTSQVHLRLISGERITTLELDASLRVTPSSALMGD
                     LKALLGPGCLGG"
BASE COUNT         1950 a         3715 c         3558 g         2032 t
ORIGIN      
        1 gttccacggt gacttcgatt tcagccaggc gttgttctcc tatgacctca acgccgacga
       61 cctggcgtcg ggaggcaccc cgccgggcat cgattcgctg ggccacacca tgaccagcgt
      121 ggtcatcaat ccgtttttcc agtgggcgtc ggaccgcgcg ccgcgcacgc cctaccacga
      181 gaccatcatc tacgaggccc acgtcaaggg catgacgcag acccacccgg gtattcccga
      241 ggaacagcgc ggcacctacg ccggtctcgg ccatccggtc gttatcgacc acctcaagtc
      301 tctgaacgtg accgcgatcg agctgatgcc cgtgcaccag ttcctgcacg accaccggct
      361 gctcgacctc ggactgcgaa actactgggg ctacaacact ttcggcttcc tcgcgccgca
      421 ctaccagtac gcagccaaca aacacgcggg cggcgcggtc gccgagttca agacgatggt
      481 gcggtccttc catgaagcag gcatcgaggt cattctcgac gtggtctaca accacaccgc
      541 cgagggcaac cacctcggcc ccacgatcaa cttccgcggc atcgacaacg ccgcctacta
      601 ccgtctgcag gacggcgatc tcagggtcta caaggacttc accggcaccg gcaacagcct
      661 caacccgcgt catccgcaca cccttcagct gatcatggac tcgctgcggt actgggtgct
      721 agagatgcac gtcgacggct tccgcttcga cctggcggcc gcgctggccc gcgagttcta
      781 cgacgtcgac cgactgagcg cgttcttcga tctggtgcag caggatccgg tggtcagcca
      841 ggtcaagctg atcgccgagc cgtgggacgt cggcgagggc ggctatcagg tcggcaactt
      901 tcctggtttg tggaccgagt ggaacgggaa gtatcgcgac actgtgcgcg attactggcg
      961 gggtgagccc gcaacactcg gcgagttcgc ctcgcggctg accggatcct cggacctcta
     1021 cgaggccacc ggccggcggc ccggcgcgag catcaacttc gtcacctgtc acgacggctt
     1081 cccgctggcc gacctggtct cgtacaacga gaagcacaac gaggccaacg gcgaagacaa
     1141 ccgcgacggc gaaagccaca acaggtcgtg gaactgcggg gtggagggcc caaccgacga
     1201 cccggacatc ctcgccctgc gcgccaagca gatacgcaac atcatgggga cgttgatggt
     1261 ctcccagggc actccgatga tctcgcacgg cgacgaaatc ggacgcactc agcggggcaa
     1321 caacaacgtc tactgccagg attccgaaat ctcctggatg gattggtcgt tgtgtgagaa
     1381 gaaccccgac ctgctcgagt tcacccgccg agtcaccaag ctgcggcgga accacccggt
     1441 cttccgcagg cgtcgcttct tcgagggcga gccgatccgc acgggcgatc aggtccgcga
     1501 catcgcctgg ttgacgccag ccggcaagga gatggcccgc gaggattggg gcaccgggct
     1561 gggcaagtgc gttgcggtat tcctcaacgg cgaggcgatc ccgacatcca atgcgcgcgg
     1621 cgaacgggtt gtcgacgatt cattcctgtt gtgcttcaac gcaaacgaca agccgcagga
     1681 cttcgtcacc ccgaagggtg attacgccaa ggagtggacg gcgacgctgg acacggccga
     1741 cgccacgggg tcgaccgagt tggttgtcac tgcaggcaag aagatttcac tgcaggcgcg
     1801 gtcgctgctg gtgctgcgta agaccgcctg actcatggtc tttccggtgc tgtcgacgta
     1861 ccgactgcag atgcgcggcg actgtttcac gttcgacgac gcggtggagg agctggacta
     1921 cctcgacaac ctcggggtgt cgcatctgta cttgtcgccg atcctgaccg ccgcgcaggg
     1981 gtccactcat ggctacgacg tcaccgaccc caccaccgtt tcggctgcgc tgggcggacc
     2041 cgacgggcta gcgcggctgt ctgctgcggc caaggcccgc ggcatggggc tgatcgtcga
     2101 catcgtgccg aatcacgtcg gcgtcgacaa ccccgaacag aacgcgtggt ggctcgacgt
     2161 gctcgcgcac gggcgctcat cgccgtacgc ggcgtacttc gatatcgatt gggacctcga
     2221 ccccgagagc cgaattatct tgccggtgtt gggctcagac ggcgaccgtc ccccgccagg
     2281 ttacccagtc gatcagtcgc actaccggcc gatcggctgg cgcaacggca tctgcggcta
     2341 ccggcggttc ttttcgatca catcgctggc tgggctgcgc caagaggacc gagcggtgtt
     2401 cgacgccact catgtggagg tcaagcgctg gatcgccgag ggcttggtcg acgggcttcg
     2461 tatcgaccat ccggacggat tgtccaatcc cgcacagtat ttggcttggc tgcgcgagct
     2521 ggtcggacct gacgcgtgga tcgtcatcga aaagatcctc gccgtcgacg agccgctgga
     2581 ggtgacgctg cccgtcgccg gcacgaccgg gtacgacgca ctgcgcgaaa tcggcggggt
     2641 actcgtcgat cccgccggcg caggtgcact gacagcatta ttcgaatcga gcggtgtcgc
     2701 ctacgacgaa atgccgcaat tggcccgtgg cctcaaggcc gcggccgtaa cggacacgtt
     2761 gggcagcgag ctggcccggt tgcaccggac catcgtcgcc gaagccgggg ccgaccaccc
     2821 cgacctgccc cgggccgtgg ccgagttgct gagcagggtg catgtgtacc gatccgacta
     2881 cctgtcattg tccctggtgc tcccggaggc cgttgccgaa accgtctctg ccttaccgga
     2941 actggcggag ccgttggcga ttattgccac cgcggtgacg tccagtcgtg agtcggcggt
     3001 gcggctgcag cagctgtgtg gcgccgcgac cgccaagtcc atggaggact gcctctttta
     3061 ccgcgacgcc cggctggtgt cgctgaacga ggtgggcgga gagcccgccc ggttcggagt
     3121 gagcgtcgca gagtttcacg agcgcgcagc tacccggtct tccttgtggc cgcatacgat
     3181 gatcgcgttg accacccacg acaccaagcg cggcgaggac gtgcgcgctc ggatcggcgt
     3241 gctgtcgcag gtgccatcgc tgtgggcgga actcgtgggc aagtgggctc tagaggcgcc
     3301 gccgcccgat cccgcgaccg gactcttctt gctgcagaac atcttcggtg tatggcccgc
     3361 agacggtgtg gtgaccgatg aactacgcgg gcgggtgcat acctacgccg aaaaagcaat
     3421 ccgggaggcg gccgtccaca cgtcatggca cgaccctgac cccgctttcg aggctgcggt
     3481 acacgcgtgg ctggacgctg tcatggacgg accggtcgcg gcacagttga cctccctggt
     3541 gacgcggttg gacgtatatg ctcgcagcga cagcctgagc cagaagttgt tggcgctcac
     3601 cgtgccaggt gttccggacg tctaccaggg taccgaactg tgggaggaca gtctggtcga
     3661 cccggacaac cgacgaccgg tcgactacgg cgtcaggcgc gaggcactga agatcatgac
     3721 ccaccccaag gttcgggtgg tggcggcggc cctgcggctg cgcaggcaaa gctcggccag
     3781 cttcaccgac gctggttaca caccgatcct tgccgagggc cccgccgctg agcacctggt
     3841 ggcgtttctg cgcggtgccg atgtgttgac tacggtcacc cggcacccgg tgcggctgtc
     3901 tgaaattggc tggggcgaca ccgctctgga tcttccaagc ggcacgtggg ttgaccggat
     3961 cggtggcaga caatacagcg gcagggtctt ggccgccgag ttgtccgcgg agctgccagt
     4021 tgcgctgttg gagcgtatcg atggttgagt tcgcggtgtg ggcgccgcga ccggagcggg
     4081 ttcgtctcga cgtcgacggc acgctgtatc cgatggaccg gtccgacgac ggctggtggc
     4141 gaaccgacgt cggcgcccaa cccgatgcac gctacggctt cgtgctcgat gacgacccaa
     4201 aggtgctgcc ggatccccgc tctccccgcc agcccgacgg cgtgcacgag cgcgcacagc
     4261 ggtggcagcc cgccgccgac acttggaccg atgccgattg ggaaggccga tcgatcgagg
     4321 gccgggtcat ctatgagttg catgtcggca cgttcacgcc gggtggcacg ttcgacgcgg
     4381 cggtcgagaa gctggattac ctggtcgatt tgggcatcga cttcgtcgag ctgatgccgg
     4441 tcaatgcgtt cggcgggaca catggctggg gctacgacgg cgtgctctgg tatgcggtgc
     4501 acgaaccgta tggcgggccc gacggactcg tgcgcctgat caacgcctgc caccggcgtg
     4561 ggctcggcgt gttgatcgac gcggtgttca atcaccttgg cccgtcgggc aattacctgc
     4621 cgcattttgg gccgtacctg tcgtcgggta gcaatccgtg gggcgaatcg atcaacctct
     4681 gcgacgccga cgccgacgcg gtgcgtcgct acatcatcga ctgcgcgctg cggtggatgc
     4741 gcgagttcca tgccgacggg ctgcgactgg acgctgtgca cgcgctcgtc gacaccaccg
     4801 ccattcacat tcttgaagaa ctggccgccg aaaccgattc gctcgcagag gagttgggcc
     4861 ggccgttgtc gttgatcgcc gagagcgacc tcaacgaccc gcggctgatc acgccgcgcg
     4921 accgcggtgg atacgggatg accgcgcagt gggacgatga catccaccat gcgatccact
     4981 cggcggtgtc gggtgaacgt cagggctatt acgccgattt cgggtcgctg gaaacgctgg
     5041 cgcaaacatt gaagcacggc tactttcacg cagggaggta ctcgtcgttc cggcatcgcc
     5101 gccacggccg tccactggac acagcgagca tcccggcgac gcggctgctg gcgtacacca
     5161 tgacgcacga tcaggtcggc aaccgggcgg tcggcgaccg gccgtcgcag aatctgacgt
     5221 tcggccagct tgcgatcaag gccgcgctgg cactcggatc gccctatacg ggaatgcttt
     5281 tcatgggtga ggagtggggg tcgtcgtcgc cgttccagtt cttcagctcg catccggaac
     5341 cggagctggc aagggcgacc gccgagggtc gcaagcgtga gttcgccgaa catgggtggg
     5401 acgccgacga gatacccgac ccgcaggacc ccgcgacgtt cgaacgatcc aagctgaatt
     5461 gggacgagat cgacgacggc gagcatggcc agctgcggcg ggtctaccga caactgatcg
     5521 cattgcggca caacgaacct gacctggcag acccgtggct ggatcacatg cgtgtcgact
     5581 atgacgaaga cggacggtgg atcgtaatgt accgcgggtc cttggcgatc gcatgcaacc
     5641 tcggcacgga cgtcgtcgac gttccggtca ccggtgagct cgtgttgacc tggggcgagc
     5701 cgaaagtcga cactgagacc acgcgactgg aggggcattc gttcgcagtg ctccgacgag
     5761 tgtcggggtg atgcacgctt tcagtccgat ttcgtgtcac aacccaacac tcgagtctca
     5821 gagcaggtag cgatacgccg gcgaacccgg ctccagggtc tcgacgtgga tgtcggatgc
     5881 cttcattcgc tccagcagac cctcgagatc ggcggccgaa cccaactcga tgccgaccaa
     5941 ggcctcgccg gtgtcgcggt tgttgcgctt gacgtactcg aacaacgtga tgtcgtcgtt
     6001 gggcccgagc accgtgtcga ggaatcgccg cagcgctccc ggctcctgcg ggaagtccac
     6061 caggaagtag tgcttcagac ccaggtgcac gagcgaacgc tccaacacct cgttgtagcg
     6121 ggagacgtcg ttgttgccac ccgaaatcag gcacaccaca gtggattccg ggtcgatgtc
     6181 ggcctccatc agcgcagcca ccgacagcgc ccccgccggc tcggcgatga tgccctcgtt
     6241 ctggtacagg tcgagcatcg cagtgcagac agcaccctcg tcgaccgtgg tgatcgacac
     6301 catgtcgccc gccgcggcca gcgccccgta cgtcagcgtg ccggcccggt tcaccgcggc
     6361 gccgtcgacg aactggtcga cgtgttcgag cgtgaccggt tccccagcgg ccagcgccgc
     6421 gatcatcgct gcggctccgg ccggttcgac gcccaacacc gatgtgttcg tcgtccgctc
     6481 ggccagatac gtcgtgatgc ctgcgataca accgccgcca ccgaccggga cgatcaccag
     6541 gtccggctcg ccctcgagtt ggtcgagaat ctcggccgcg atggtgccct gcccggccat
     6601 cgtccgcgga tcgtcgtacg gcggcaccag cgtcgcaccc gtcagtgcca cgtcgtcgag
     6661 cgccgccgcg gcggcctcgt cgtaggtctt gccgatcgcg atcagctcga tgaactcgcg
     6721 gccgtgatag cggatgcgat cgcgcttctg cttcggcgtc ttggcgggca catatacccg
     6781 gccgtggatg cccattgagc ggcacgccat cgcgaagccc tgcgcgtggt tgccggccga
     6841 cgagcacacc acgcctgcgg cgatctcctc gtcggacagc tgcatcagca ggttgtgcgc
     6901 gccgcgcaat ttgtacgagc ggaccgcctg ctggtcttca cgcttcagat acaccgtcgc
     6961 gccggtggcc tccgagagcc gttcgctgaa ctgcagcggg ctgcggagaa ccacgccgga
     7021 aattcgctga gcggcctcgt caatgtcggc cgcggacagc ggcgacgtgc gtgggctctg
     7081 gctcagttca gcggacaccg aacaatagtg ccactaacca gcggttttca gttaatcggg
     7141 gtgaggacga acaccggaat ctgccggtcg gtcttcgttt ggtactccgc gtagtccggc
     7201 cacgccgcga ccgcacgctc ccaccagaca gccttctcgt cgtcgaacac ctcgcgcgcc
     7261 tcgtagtcct tgtttaggtc gccgtcctgc agctccaccc gcgggtgcgc cttgatgttg
     7321 tagtaccaga ccgggttctt cggagccccg cccagagacg ccacgaccgc gtattggccg
     7381 tcatgctcga cccgcatcag cggggtcttg cggaccttgc ccgtcttggc gccgatggtc
     7441 gtcagcagga tgaggggctt gcccttcatc tcgatgccct cggtgctgcc ggactcggtg
     7501 attctgtcgg cgtccttgcg gacccagtcc cacgggcttg gttcgtactc tccggaaagt
     7561 ggcatgcgac cagcttagac gcgctaggaa agtgaccatc ctgatccggt gtttcggttg
     7621 cccgaaaact cacttgcgag ctatcgtcaa catatgaccg cgcgcgacga agtcagcatt
     7681 gccggcgttg cctggccggt atataagctg ctcgcgctcg cgatcgggtt cttggtcctg
     7741 gtgatcgtcg cggtcgcgac cacgagcgcg gcgccgtcgg tgctggcggc cgcggccgca
     7801 ggcgcggtgg tatggctggc gttgagcccg ttccacccgt cggaccgcta gttctccagc
     7861 agcgtccgca gcgtttccag gtcggcggcc accgccgccc cgtcggcgtc gaactcctcg
     7921 tccgtcatgc ctgcccgtcg tcgcacgctg aacacaactt cgcagcaggc ctctccgacc
     7981 ccgccgggga tcacccgcag cgggttgtag accgcttcgc ccgacggcag ccgaaccaca
     8041 tgatcgagca cgccgaactc gttcggcggc gcgaactcga ccgtcacctg ccccatcggc
     8101 gaatcggcta cccaaccgtc cgcggtccgc cgcagcccgc cctcggcaag tccggcagcc
     8161 cacttaggcc acgcctgcgg gtcggcggcg aactcataga cggcttcggg gtcggtctcg
     8221 atccagatgc tgacgtgtcg tgactccatc tctccatcgt ggcgcacaca ctggcctgat
     8281 ggacaccaca cccgagccct cgaaggcggt cgaggagttc gtcaaacgcg ccgagcggct
     8341 ggccaccggc gccgagcggc acatgccgtc gtggctgcgg cccggcgatc ccgaaagccg
     8401 attgcccgtg ctgttcgcac tcgttgccgt gatcgggctg cagctggcaa tcccaaagag
     8461 ctacacctta gtgccccgct ggccactgat cgggctcgag ttgttgctgc tcgcggtgct
     8521 gacctggctc aacccgctga agctcaccaa cgccactcga ctgggccgat acgccacctg
     8581 ggtgctgctc gccgccatca ccctcgacaa cacgctgtcg gcggtgctgc tggacgttcg
     8641 catcctcacc ggcgaggtca gcaacaaggc cgccgtgttg ctgggcagcg gcggggcgat
     8701 cttcgtcacc aatatcatcg tgttcggcat ctggtactgg gaactggacc gcggcggacc
     8761 gttcgcgcgg cgcgccggcg aaaagcccta cccggacttc atgttccccc agatggatcc
     8821 cgacaccgcc aagctcgtca aacctggatg gcggccgaca ttcgtggact acctgtatgt
     8881 cagcctgacc aacgcgatgg ccttctctcc caccgacacc atgccgctgg cccgatgggc
     8941 gaagggaatg atggcggtgc aaagcctcgt cgcggttacc accgtcgcgc tcgtaatcgc
     9001 aagggcggtc aatgttttgg gctaaccgtc agcccgggcg cgatcttccg gtacgggcat
     9061 acgggagcgc agaatgccaa cgacggtgtc gcgcagagcg ttcgacagct tgggctgctg
     9121 atcaggcttg atttcttcca gcgcctccgc ggccgcctgc aggatcgtac gttcttcgtc
     9181 cggcaagctg ctgaggagcg ccaccggatc gagcggacca acgtctgcga cttctacata
     9241 ccgccgcagg atgtctttcc accgcctcag gcaggcctgc tcctgcagaa tcaggaacgc
     9301 cggccattcg atatccgacc cagacagaca taacgacgtc gtcggcccat cacgcggggg
     9361 caaatactgt tgaccgtgtt cccggtaaat ggcctgaacc gagaaacacg cttgaggtgc
     9421 gccggcgacg ctcgtgaagg aaatctggtg gaataagccg cccaccgggc aggaatacaa
     9481 caccgacggc gcaccaggac acggatcgcc gggaagctgg gtgaacggcc cataaccggg
     9541 atcgatgact ctgatgccgt ttatcaacca gccgacgtag ggatgtccag tgtgttcgaa
     9601 agccaatgtc actgtttggc tgaacgtttc tttccacccg atccacacac cgtggcgaca
     9661 tgcgcgcgga tgtttgtcat agtcctcggt gactaggtgg ccacccgcgt tgctcttggt
     9721 gtcgctcacc actttcaatt cgtcggccat gtcccctccc gcgcttctgc cagagccgtc
     9781 cccagtaagc attgttctta cgagtcgtgc ctattgggcg gaattgcgcg aagtgcgaca
     9841 gacagcagcc gagacgatgg cttcgtctag ccgcccagac agcccgggcc tagcagcgcc
     9901 ttgaggtcac ccatcaacgc cgacgacggc gtcacccgca gcgacgcatc gagctcgagc
     9961 gtcgtgatcc gctcaccgct gatcagccgc aagtgaacct gcgacgtgcc cggatggcgc
    10021 gccaacacct gcttgagcgc cgtgaccttg tcgacggtgc actgccgcgt cggcaggctc
    10081 accgccaacg gccggttgac ctgggcactc gagaagtcgg gcaccacaag ctcgttggcg
    10141 atcagcgaga tccggtcatc gcggattgcc acctttgccc caaccagcac cacggcatca
    10201 tcagcgatct cggcgccgaa cgtcgaatag gtctgcggga agaacagcac ctcgatgccg
    10261 ccagtgagat cctccaattg cgctgatgcc caaggcaacc cgttcttgtt cacccgccgg
    10321 ttcaccgacg ccaggatgcc gcccacccgc acctgcgtgt cgttggcgac gtctccgtcg
    10381 aggatcgccg ggatttgtgt atcgacctgc gcggtcagca ggtgcgcgat gccgttcagg
    10441 ggatgcccgg acacgtagag gcccagcatc tcgcgctcga gcgccagctt gtgcttgtct
    10501 tcccactcct catcgggcac cttgatcgtg aacaccgcgt cggtcgcggt atcggcgccg
    10561 ccgaacaggt cgaactggcc catcgcctcg gctttcttgg ttccaagcac cgagtcgacg
    10621 gcgtcggtgt gaatcaggaa cagacccttg cgcggatggt ccagtgaatc gaatgcgccc
    10681 gacttgatca gcgactcggt gaccttcttg ttgcaggggc cgatctcgat cttgttcagg
    10741 tagtcggaga agtcaatgta cttgcctttg tcggtgcgcg tactgatcag cgacgcaacg
    10801 acattcgcgc cgacgttgcg caccgcgcca agtccatagc ggatgtcctc gccgaccgac
    10861 gcgaagttca ggcccgactc gttgacgtcc ggcggcagca cggtgatacc cagcttgcgg
    10921 cagtcggcca ggtacaccgc ggccttgtct ttgtcgtcgc ccaccgaggt gagcaatcca
    10981 gccatgtact ccgccgggta gttggccttc aggtacgccg tccaatacga cacgaggccg
    11041 tagcccgcgg cgtgtgattt gttgaacgca tagtcggcga acggcagcac cgtgtcccac
    11101 agcgccttga tggccgcggc cgagaagccg ttggccttca tgccatcgga gaagccctcg
    11161 tactccttgt cgagtacctc acgcttcttc ttgcccatgg cttttcgtag aatgtctgct
    11221 cgggcgagcg agtagctggc caccttctgc gcgat
//
LOCUS       DASRJA010000344         3369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3351981,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000344 DASRJA010000000
VERSION     DASRJA010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3351981"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VFC01_15235"
     CDS             <1..785
                     /locus_tag="VFC01_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005245656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HZN81018.1"
                     /translation="TLTEHLGAAAGEIDRLVRLSPQGKAELTAGWLQHETGANWKLLA
                     ENETDGYHPQFVHGSIFGVTGSTIGPLYSDASTAVTRNLGNGHSENDLRPEFRKFAEP
                     MRWFGTTEQRVPNYVTAMRELHGEAAEQILIEGAPHVMIFPNLFIAEIQVFNIQPVSV
                     NQCVQYSTAVQLAGAPELNQRMVSQCVGSVGPAGMLLADDTEMYERNQLGIEALQPEW
                     LDVRRGLHRERVDDNGFTIGSATDETGMRGFWSHYASMMEAP"
     gene            794..1330
                     /locus_tag="VFC01_15240"
     CDS             794..1330
                     /locus_tag="VFC01_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HZN81019.1"
                     /translation="MDTDANATDALVDIPGGYDLREVEQFLYREARFADESDYDSWEA
                     LWTDDALYWVPAGNDDAHPLEQMSVIYDNRNRISTRLKQLRTGKRYAQAPPSNLRRMI
                     NNVELLGGRAAESIAEPDLEVGANFLVLESRPRGNHLWGGRVTYRLRRVEGALRMSYK
                     KVVLVDKDKPIPTLGFLI"
     gene            1330..1584
                     /locus_tag="VFC01_15245"
     CDS             1330..1584
                     /locus_tag="VFC01_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019509844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81020.1"
                     /translation="MSIEFSGSGPVDVPGIGPVVESEFARVSVNVDDQGNSPRLRVED
                     LRTGRVRYLDALELETIVWLPEGMLAQLLDPSADRWRGDE"
     gene            1581..2792
                     /locus_tag="VFC01_15250"
     CDS             1581..2792
                     /locus_tag="VFC01_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZN81021.1"
                     /translation="MRRAAIVAPVRTAVGTFGGSMRPLRAEDLAAKVVEATVTRSGVD
                     PSLIEDVVFAQSYANSEAPCIGRWVAMHANLPVAVPGLQIDRRCGGGLQAVITAAMTV
                     QTGAADVVIAGGVESMSNIEHYTTTARWGARTGNQLLYDRLDRGRERSQPAWRFGEIS
                     GMIETAENLASDYGINRDAADAFAARSHERAAAAAQRGAFDDEIVPVEVPQRRSDPVL
                     VEHDEGIRPNTTVDSLAKLRTVMPNGTVTAGNAAQQNDAAAACLLVAEDKLEELGLEP
                     IGYLEGWAAVGCEPSHMGIGPVGAVEKLFSRTGFGFDELTLIEINEAFAVQVLAVLSG
                     WGVKLADVDDRLNVNGSGISLGHPIGATGVRILTTMLHELQRRGGGLALETMCIGGGQ
                     GIAALFRGVAR"
     gene            2789..>3369
                     /locus_tag="VFC01_15255"
     CDS             2789..>3369
                     /locus_tag="VFC01_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HZN81022.1"
                     /translation="MTSLIAYATYLPRYRLAGADVGLRRGDRVVASYDEDSTTMAVAA
                     ATRAVKGHPLPTNLYFATSSPAYADKTNAAAIHAALGLPFAAFATDLGGTGRSGFAAW
                     RSAAATGGLAVTADVRVGRPGSADERLGGDGAAALLFGDGPAIADIVTTTSFTAEFLD
                     RWRSPRSITGEQWEERFGADRYSALIRTAAERIL"
BASE COUNT          599 a         1124 c         1128 g          518 t
ORIGIN      
        1 ccacgctgac cgaacacctc ggggcggccg cgggcgagat agatcgactg gtgcgacttt
       61 ctccgcaggg caaggccgag ctcaccgcgg gctggctgca acacgagacg ggcgccaact
      121 ggaaactctt ggcggagaac gagaccgacg gttatcaccc ccagttcgtg cacggctcca
      181 tcttcggtgt cacgggcagc accattggcc cgttgtacag tgacgcctcc acggccgtga
      241 cgcgcaattt gggcaacggg cacagtgaga acgaccttcg ccccgaattc cgcaagttcg
      301 ccgaaccgat gcggtggttc ggcaccactg agcagagggt gcccaactac gtcaccgcga
      361 tgcgcgaact tcacggtgag gccgccgagc agatcctgat cgagggcgcc ccccacgtga
      421 tgatcttccc caatctcttc atcgcggaga tccaggtctt caacatccag ccggtctcag
      481 tcaatcagtg cgtccagtac tccactgccg tgcagctcgc cggagcaccg gaactgaacc
      541 agcggatggt gtcgcagtgc gtcggatcgg tggggccggc gggcatgctg ctggccgatg
      601 acaccgagat gtacgaacgc aatcaactcg ggatcgaggc gctgcagccg gaatggctcg
      661 acgtgcggcg cggactgcac cgggagcggg tcgacgacaa cggcttcacc atcggctcgg
      721 ccaccgacga aaccggcatg cggggcttct ggtcgcacta cgcatcgatg atggaggcgc
      781 cgtgaccaac gccatggata ccgacgccaa cgccaccgac gccctggtgg acataccggg
      841 cgggtacgac ctccgcgagg tcgagcagtt cctctatcgc gaggcacgtt tcgccgacga
      901 gagcgactac gattcctggg aggcgttgtg gaccgacgac gcgctgtact gggttccggc
      961 gggcaacgac gatgcgcatc ccctggaaca gatgtcggtg atctacgaca atcggaaccg
     1021 aatctccacc cggctcaaac aacttcggac cgggaagcgc tatgcccagg cgcctccgtc
     1081 gaacctgcgt cggatgatca acaacgtcga gctgctcggc ggccgcgccg cggagtcgat
     1141 cgccgaaccc gatctcgaag tgggggcgaa cttcctcgtc ctcgagtctc gcccccgcgg
     1201 caaccacctc tggggcggcc gcgtgacgta ccgtctccgc cgcgttgagg gggcgctgcg
     1261 gatgtcctac aagaaggtgg tgctcgtcga caaggacaag cccataccga cgctgggatt
     1321 cctgatctga tgagcatcga attcagcgga agcggacccg tcgacgtacc aggaatcggt
     1381 ccggtggtcg aaagcgagtt cgcccgcgta tcggtcaacg tcgacgatca ggggaactcg
     1441 ccaaggctgc gcgtggagga cctccgcacc ggccgggtcc gttatctcga cgccctggag
     1501 ctggagacga tcgtgtggtt gccagaaggc atgctggcac agctgctgga tccgtccgct
     1561 gaccgctggc ggggggacga atgaggcgcg ccgccatcgt cgcgccggtc cgcaccgcgg
     1621 tgggcacgtt cggcggaagc atgcgccccc tgcgcgccga ggacctcgcc gccaaggtcg
     1681 tcgaggcaac cgtgacacgt agcggtgtcg acccctcgct gatcgaggat gtcgtcttcg
     1741 cgcagtccta cgccaactcg gaggcgccgt gcatcggccg ctgggtcgcc atgcacgcga
     1801 accttccggt cgcggtgccg ggtctacaga tcgaccgtcg ctgcggcggc gggctgcagg
     1861 ccgtcatcac cgccgcgatg acggtgcaga ccggcgctgc cgacgtcgtc atcgccggcg
     1921 gcgttgaatc gatgagcaac atcgagcact acacgaccac ggcgcgttgg ggtgccagaa
     1981 ccggtaacca actgctctac gaccggctgg accgcggccg cgaaagatca cagcccgcat
     2041 ggcgtttcgg cgagatcagc ggaatgatcg aaacggcgga gaacctggcg tcggactacg
     2101 gcatcaaccg agacgccgcc gacgcattcg cggcccgcag ccacgaacgc gcggctgcag
     2161 ccgcccagcg cggcgcgttc gacgacgaga tcgttcccgt cgaggtgccg caacgccgga
     2221 gtgacccagt gctcgtcgag catgacgaag gcatccgacc caacaccacg gtcgactccc
     2281 tggccaagct ccgcaccgtg atgcccaacg gcacggtgac cgctggcaac gcagcgcagc
     2341 agaacgacgc cgcggcagcg tgcctcctcg tcgcagagga caagctcgag gaattgggac
     2401 tggagccgat cggctacctc gagggctggg ccgcggtcgg atgcgagccg tcacacatgg
     2461 ggatcggccc cgtgggcgcc gtcgaaaaac tcttcagcag aacgggtttc ggattcgatg
     2521 agctgaccct catcgagatc aacgaggcat tcgcggtcca ggtgctcgcc gtcctgtccg
     2581 ggtggggcgt caagctcgcc gacgtcgacg acaggctcaa cgtcaacgga tccggcatct
     2641 ccctcgggca tccgatcggc gccaccgggg tgcgcattct caccacgatg ctgcacgaat
     2701 tacagcgcag aggcggcgga ttggccttgg agaccatgtg catcggcggc gggcagggca
     2761 tcgccgcgct gttccgggga gtcgcacgat gacctcgctg atcgcctacg ccacgtatct
     2821 gccgcggtac cgcctggcgg gtgcggatgt cgggctgcgg cgcggagacc gcgtcgtcgc
     2881 gtcctatgac gaggacagca ccacgatggc agttgccgcg gccacgcgtg cagtcaaggg
     2941 gcacccgctg ccgaccaacc tctacttcgc gaccagcagc cctgcctacg ccgacaagac
     3001 caacgcggcc gccatccacg cggcattggg tctgccgttc gccgcgttcg cgaccgattt
     3061 gggtggcacc ggccgcagcg gattcgccgc atggcgctcc gccgccgcga cgggcggcct
     3121 cgcggtgacc gctgacgtgc gcgttggcag gcccggttcc gccgacgaac gactcggcgg
     3181 ggacggcgcg gcggcgctgc tgttcggtga cggtccggcg atcgccgaca tcgtgacgac
     3241 gacgtcgttc acggcggagt tcctggatcg ctggcgttcg ccccggtcga ttacggggga
     3301 gcagtgggag gagcgcttcg gtgcggatcg ctactcggcg ctgattcgca ctgccgccga
     3361 acggatcct
//
LOCUS       DASRJA010000345        19460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1297982,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000345 DASRJA010000000
VERSION     DASRJA010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 19460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19460
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297982"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFC01_15260"
     CDS             complement(<1..323)
                     /locus_tag="VFC01_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase"
                     /protein_id="HZN81023.1"
                     /translation="MPIIEQVGAREILDSRGNPTVEVEVALLDGTFARAAVPSGASTG
                     EHEAVELRDGGSRYGGKGVEKAVQAVLDEIAPAVIGLSADDQRLVDQALVDLDGTPDK
                     SRLGA"
     gene            637..2061
                     /locus_tag="VFC01_15265"
     CDS             637..2061
                     /locus_tag="VFC01_15265"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HZN81024.1"
                     /translation="MSRHTTFRFCLDPTVEQREVLARYAGASRFAFNQCLHMVKTALT
                     QRKNDPDIQVLWTGFDLINAFNAWKKTEDAGRVFTVNTRGEAEIVVTDLRWCSEVCQQ
                     VFEEAAVDCGRALAAWSDSRSEGKGNRVGFPRFKKKTSATPSFRLRNKFRKSRRPAIR
                     VGDNDRSRSITLPGIGQIGVHNDTRRLRRMISNGRAKILFATVTHHGGRWWISLNVAA
                     AELHAAHLHPVRDPGDAGGWVGIDRGLSAFAVAATRDGEEVARITYAPKALNAGMKRQ
                     RRLAKSLSRKQKGSRNRRGAAAKLGRHHNHVANVRRHFLHQVSNELVKTHDRLVIENL
                     NVSGMLANHRLARAISDAGWAEFARQLSYKQAWRGGEVVIADRWYPSSKLCPACGAIH
                     VNLMLADRVFTCGCGRSADRDLNAAVNLARWGQQHHLDPRTPKQRGRATNARRRDGAD
                     QHPTRAGETSPVEAGTDVHTAPAA"
     gene            complement(2145..2873)
                     /locus_tag="VFC01_15270"
     CDS             complement(2145..2873)
                     /locus_tag="VFC01_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZN81025.1"
                     /translation="MSPVRWLRAVAVVGATALLMASSCSWQLGTPIPEGVPPPAGDPV
                     PKIDTHAKGRPADQLHEWAAQRAPALGMPVAALEAYAYAARVAEVENPDCHIAWTTLA
                     GIGQVESHHGTYRGAVIGPKGDVTPPIRGVRLDGTNGNLEIIDNDGDADGDPQYARAM
                     GPMQFIPETWKLYGVDANNDGIISPDNFDDAALSAAGYLCWRGKDLAKPRGWMDALHA
                     YNHSDQYARTVRDWATAYANGHPL"
     gene            3049..3963
                     /gene="efeU"
                     /locus_tag="VFC01_15275"
     CDS             3049..3963
                     /gene="efeU"
                     /locus_tag="VFC01_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HZN81026.1"
                     /translation="MTAGTGVGVSIQAAAPNITSQLFGSGLIGLREGLEAAIVVTILV
                     AFLVKSEHRDALKWVWLGVAAAILMTVAVFLVIQFGENTISGLAAEAIAGIASLVAVA
                     IVTTMVIWMKKAAASMSGELRSEMARALETGSLAVLALAFFAVGREGVETALFMVGYA
                     EAQTSWPLIGLVIGVLAAATIAYGMYAGAVRINLAKFFKYTGVFLIVVAAGILSYGVG
                     ALQTVGWLPGLSAKAFDISTWFNWSSWYGEVVQGVFNVTPTPTVLQLAAWLAYLVIVL
                     TIFLRPTKAPAKPDPSPQPLPESERSTT"
     gene            3960..5117
                     /gene="efeO"
                     /locus_tag="VFC01_15280"
     CDS             3960..5117
                     /gene="efeO"
                     /locus_tag="VFC01_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HZN81027.1"
                     /translation="MKLYSSLAVATAALAGLALTSCQAKEEAKPADPGKSAPSEITVT
                     ASDSTCELSGTEASTGPSIFVITNNGDKVTEFYVYGEGERVMGEVENISPGLQRKLIV
                     QLAEPGTYQTACKPGMVGDGIRGDFKVTGDAVKVDTEGKFKEASDSYKRYVNSQTDAL
                     IAATAVFVDAVKKGDVNAAKAQYPIARTYYERIEPVAESFPNDLDPRIDLREADLEPG
                     QKWTGFHRLEKDLWVTGLQPDSNAMADQLMADVKELDNGVKAPEYKIDSTQIAGGAQG
                     LLDEVSSSKITGEEDIFSHTDLWDFKANVEGSQTAVASVRPILDERNAELGKKVDQRF
                     AEVEALLEKYRQGDGFISYDKVTEPQRQELSRAIDALSKEVSQVQGVIAPQ"
     gene            5098..6402
                     /gene="efeB"
                     /locus_tag="VFC01_15285"
     CDS             5098..6402
                     /gene="efeB"
                     /locus_tag="VFC01_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HZN81028.1"
                     /translation="MSSLPNDPTPPDERPGLSRRKLFGAAGVTAAVVGAASAGTLAGR
                     ASAASTDFGGLDKPIPFRGERQAGIITPQQDRMHFCAFDVTTDSRDDVIALLKQWTEM
                     AERMTQGEDAVPNGAIGGNPYAPPDDTGEALGLPASGLTLTIGFGPSFFEKDGKDRFG
                     IANKRPAALADLPKFTNEIMDPAKCGGDICIQACAHDPQVAVHAVRNLARVGFGTVAV
                     KYSQLGFGRTSSTTREQLTPRNMLGFKDGTNNIKAEDHDALDKNVWVAAGDGPEWLTG
                     GTYLVTRRIRNRIEAWDRTTLKEQERVIGRQKGSGAPNGYDDEFAPVDFNIKDADGIP
                     LVDVDAHIRLVSPDHLNGIKMLRRGYNFTDGSDGFGHLDAGLFFIAFVRSPATNFIPV
                     LAQMSGDALNEYIFHTGTAVFACPPGIREGDKSAYWGSTLFA"
     gene            complement(6559..7338)
                     /locus_tag="VFC01_15290"
     CDS             complement(6559..7338)
                     /locus_tag="VFC01_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZN81029.1"
                     /translation="MTKQTPSQSVVVGIDGSQAALDAATWAVAEAVNLGVPLRLVYVS
                     VAKHTCRSRVDAVSSGAEHAESALYRAEMTVHDMGKPIQIETAIVRGRPDCVLTDESR
                     RAALVCVGSEGKGPCARMPLGATAAALVKHAHCPVAIVRSGGAPQTGPGWIAAVRNDE
                     PDNDAVVHRAMEEGRLRKAPVLLIDTRLDSWIRRYPDVHVQTVAARRGGTRPIENHSD
                     SIQLAVVGSADAEHVARLSWPDYHPLLGDSNRSVLLVRHFA"
     gene            complement(7385..7756)
                     /locus_tag="VFC01_15295"
     CDS             complement(7385..7756)
                     /locus_tag="VFC01_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81030.1"
                     /translation="MNTPTMSSGSHGEQAKRTFTAALRFAGVIVAVAMLVLALGLLWV
                     SGCKSGTGDEALAQCGGVQRNLLGIGSPLILLLGGLAAFVRTYQIWRACGRWWIWQGA
                     GWFLLVLMLVVLTMTVPVALH"
     gene            complement(7989..8882)
                     /locus_tag="VFC01_15300"
     CDS             complement(7989..8882)
                     /locus_tag="VFC01_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HZN81031.1"
                     /translation="MREEEAKMIVDGAHSVVGDLSQTEFTPAHTDFARFLLPYQCATS
                     ALTSKVNILKRELTHADHYCPIRAVTSRVKSPNSILGKAARIGCPLTADDIGTNVLDI
                     AGVRVTCGLISETYRVATLLSQQADLTVIEVEDYIAHPKPNGYKSFHMVLEIPVYLGD
                     RVERVPVELQIRTIAMDLWANLEHKIHHKHEHTVPPRLLNDLIDAADAAHRLDVTMQR
                     LYDEVDSTLVLPFPVELQRFLEKATPQPSGAPETAGRTPAEPAAQPNGETPHVDAPPI
                     PDDPRSLPLETVAGQPFRLNQ"
     gene            8936..9733
                     /locus_tag="VFC01_15305"
     CDS             8936..9733
                     /locus_tag="VFC01_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZN81032.1"
                     /translation="MGEEADSKAGRSRRDRLPRNRQRERVLQMVRAHGDAIDAVELAS
                     RMGLHVTTVRFHLDALCDEGTIERTRMNRDGVGRPRTGYRAVEERLDYRILAEILAME
                     LGETVETRARRAQRVGVQWAGRIAGSQAEPVAGQRVADTEQALDPLDRGAVLATEVFD
                     RMGFDPELAAESEPMASLSADSKQIVGRERVIRLRGCPVRDLARAHPEVGCGIHLGLL
                     QGLLDHAAAAGGKPDTRTQGLSARLDPFVEPELCIARLHASRKSTRD"
     gene            complement(9906..11138)
                     /locus_tag="VFC01_15310"
     CDS             complement(9906..11138)
                     /locus_tag="VFC01_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN81033.1"
                     /translation="MSAQQTFVIVGAGLAGAKAAEALRTNGFDGKVILIGDEANRPYD
                     RPPLSKGYLLGSTEREKIYIHPAQWYTEHDIDLRLGTRVTEIDRAAHEVRTAQGETLG
                     YDKLLLATGSSPRRLEVPGADLPGLHYLRTVADSDALQAAFASAQRVAIIGAGWIGLE
                     TAVAARAAGCHVTLLERGKLPLLGVLGAEVAETYAALHRAHGVELRPGSGVAEILGVA
                     DKVTGVRLADGDVVAADTILIGVGILPNIELAAAAGLPIDNGVVVDEHLATIDPDIFA
                     AGDVANAYYPLLGTHLRLEHWSAALNQGPVAAANMMGAPTAYDKVPYFFSDQYDSGME
                     YSGYVPRGGYDEVVFRGDVASGKYIAFWMKGGTVLAGMNVNTWDVTDAIEALVRSAAQ
                     PDPSKLADPDVPLTELTG"
     gene            complement(11306..12412)
                     /gene="tal"
                     /locus_tag="VFC01_15315"
     CDS             complement(11306..12412)
                     /gene="tal"
                     /locus_tag="VFC01_15315"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HZN81034.1"
                     /translation="MIKLERLYTEQRQSAWLDNLTRLYLRDGTLAAYVADGIRGVTAN
                     PTILAKAIGASDLYDAQFFALTAAGYSIEDAYWELAVHDVTDALAVLRPTYDNSHGSD
                     GFVSIEVAPELARDTTATIAAATRLHERIARPNLLVKIPATAEGIPAIAATIGSGLSI
                     NVTLIFSLSRYEQVIEAYLAGLETLAARGGVLPAVRSVASFFVSRVDTQVDNRLEQIG
                     GADAAALRGRAGIAQARLAYQLFRDRFSGPRWEKLAALGAHPQSPLWASTSTKNPDYP
                     DTLYVDSLIGPDTINTMAEATIAAFEDHGTVARTIDTGMGEATDVMQNLRSVGIDMDD
                     VGRSLEDQGVAGFHQSFADLLIALRDKAHQLAQQ"
     gene            complement(12486..13235)
                     /locus_tag="VFC01_15320"
     CDS             complement(12486..13235)
                     /locus_tag="VFC01_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN81035.1"
                     /translation="MSISRPADLAALNGLSHLDDPLRRQLYEYVIESDGPVSREQAAA
                     AASIGRTLAAYHLDKLAEAGLLTVSYQRPAGRGGPGAGRPAKLYTRAAQEITVSVPPR
                     DYELLARLLVASVEQDASGAVQQAVNSAAHEAGRRAGAETGGSVVGALRNCGYLPHAD
                     DDGRIDLRNCPFHLVAADHRDVVCGLNLRLVEGVIAGCGHPHAHAELDPAPDRCCVVV
                     HDAQPSARTRRRRRPHPAVPADSETPDRPAP"
     gene            13327..13806
                     /locus_tag="VFC01_15325"
     CDS             13327..13806
                     /locus_tag="VFC01_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZN81036.1"
                     /translation="MTASALPASAAHASSGVLQRARSDPAYAAFLLLRIGFTVLPIVF
                     GLDKFTNVLTKWEVYLAPWIVDMSPISAHQVMLVVGVIEIVAGVAVAIKPRYAAYIVA
                     AWLAGIIVNLLSYPGFYDVALRDVGLLLAALTLARLAFVYDPAWHRRTTAGTDSPAA"
     gene            13900..14496
                     /gene="folE"
                     /locus_tag="VFC01_15330"
     CDS             13900..14496
                     /gene="folE"
                     /locus_tag="VFC01_15330"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HZN81037.1"
                     /translation="MHSTAALRLVHESPHRDLAAAEVAAAAFLRALGVSLDSQSLAGT
                     PARMARGYAELFTPRTFDLTTFPNDEGYDELVISRNLPLRSVCEHHMLPFVGIAHIGY
                     LPGERIIGLSKLARVAEHFACRPQRQERLTKQIADWLDAQLQPRGVGVVIEAEHTCMT
                     LRGVQAARSATITSTLLGALREDPRSRQEFFALTGANT"
     gene            14528..14911
                     /locus_tag="VFC01_15335"
     CDS             14528..14911
                     /locus_tag="VFC01_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HZN81038.1"
                     /translation="MTQRRKVKARRIYDPALPQDGTRVLVDRIWPRGVSKARAQLDEW
                     CKDIAPSTELRKWYSHDPALFEEFAHRYRDELSEPAHAAAFAHLRDLAKAGTLTLLTA
                     TKNPDLSEAAVLADLVGSGQRRPVG"
     gene            complement(15021..15482)
                     /locus_tag="VFC01_15340"
     CDS             complement(15021..15482)
                     /locus_tag="VFC01_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN81039.1"
                     /translation="MTQATEPAPIGGVTTTVASTVIRVADLDRSVSFYRDVFCCRVAL
                     REPDTALLLTPDGFQIYLHSTGPSRRPRLAPIGIEYLMWATDSQAELERVTNRLRAHD
                     PATYTYTENGLTFVEGCDPDHGRVIVAYPSPSRLPRELIASRLRGVVRAAT"
     gene            complement(15800..17545)
                     /gene="ctaD"
                     /locus_tag="VFC01_15345"
     CDS             complement(15800..17545)
                     /gene="ctaD"
                     /locus_tag="VFC01_15345"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZN81040.1"
                     /translation="MSAVVELTPTRPFPPRRGVKGSFVYRVLTTTDPKLLGIMYTVTS
                     VGFFMAGGLMALLMRSELGAPGLQFLSNEQYNQLFTMHGTIMLLLYATPIVFGFANCI
                     LPLQIGAPDVAFPRLNALSYWLYLFGALVTVGGFLTPGGAADFGWTAYTPLSDALHSP
                     GAGADLWIMGLAIAGLGTILGAVNMITTVVCMRAPGMTMFRMPIFTWNILVTSVMMLI
                     AFPLLTAALLGLAFDRHLGGHIYDPANGGVILWQHLFWFFGHPEVYIVAIPFFGIITE
                     IVPVFSRKPVFGYTTMVYATVSIAALSTAVWAHHMFAPGAVLLPFFSFMSYLIAIPTG
                     IKFFNWIGTMWHGQLTFETPMLFSVGFMVTFLLGGLSGVLLASPPIDFHVTDTYFLVA
                     HFHYVLFGTIVFATYAGIYFWFPKMTGRLLDERLGKLHFWLTFIGFHTTFLVQHWLGN
                     EGMPRRYADYLPADGFTTLNIVSTIGAFTLGASMLPFVWNIFKSYRYGEVVIVDDPWG
                     YGNSLEWATTCPPPRHNFTELPRIRSERPAFELHYPHMADRMRAEAHVGRHQHSNPAA
                     ERATPAAPTSNPSGG"
     gene            complement(17820..18107)
                     /locus_tag="VFC01_15350"
     CDS             complement(17820..18107)
                     /locus_tag="VFC01_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HZN81041.1"
                     /translation="MTLYDPHTADDADELDVRQIPKSQRHPLIFARFDALAAGESFVL
                     VNSHDPIHLREEFARDRPGAYDWRYLEGSQSDRLWRIRIVRITEPDLAGLL"
     gene            18155..18499
                     /gene="fdxA"
                     /locus_tag="VFC01_15355"
     CDS             18155..18499
                     /gene="fdxA"
                     /locus_tag="VFC01_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN81042.1"
                     /translation="MTYVIGAPCVDIKDRACVDECPVDCIYEGARMLYIHPDECVDCG
                     ACEPVCPVEAIYYEDDLPEPLRPYIDENVKFFTDTLPGQTDPLGSPGGAAKLGAVDAD
                     TPMVAALPPQGD"
     gene            18499..19191
                     /locus_tag="VFC01_15360"
     CDS             18499..19191
                     /locus_tag="VFC01_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN81043.1"
                     /translation="MPTDATAPAPGMGPPGRRDVILALLRASAQPRSIASVADELGVH
                     PNTVRFHIDALLRTGRVEQVLGDSAGRGRPPILFRASRRMDPTGPTNYRLLAAILTGY
                     VAREPDAAGIAAQLGRSTSPALVASSGQRRAPSKTRAVTELVELLAELGFEPEPAEGP
                     RTREIRLRHCPFQNLAEQHGEVICSVHLGLMQGALSAMRAPVTVDRLEPFVEPDLCVA
                     HLAPAPSRDRAS"
     gene            19188..>19460
                     /locus_tag="VFC01_15365"
     CDS             19188..>19460
                     /locus_tag="VFC01_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81044.1"
                     /translation="MTELAIRVAAAATLIWLGMVLAISFLEAPLKFRAEGLELRVGLA
                     IGRIVFRALNIVEVIWAVVIAVCLSVGGPSGPVLSLAAVTAVLLAVQ"
BASE COUNT         3392 a         6190 c         6481 g         3397 t
ORIGIN      
        1 ttggcgccca gccgcgactt gtccggggtg ccgtcgagat cgacgagcgc ctgatcgacc
       61 agccgctgat cgtccgcgct cagcccgatg accgcgggcg cgatctcgtc gagcacggcc
      121 tgcaccgcct tctcgacgcc cttgcccccg tagcggctgc cgccgtcgcg cagctccacc
      181 gcctcgtgct cgccggtcga cgcgcccgac ggcaccgcgg cacgggcgaa cgtaccgtcc
      241 agcagggcca cttcgacctc gaccgtcggg ttgccgcggg agtcgaggat ctcgcgggct
      301 ccgacctgct cgatgatggg cactggcttc tccttgatca tggggctgga ctgaattgag
      361 cctagagggt gtcgagcaac ttgttggggt ggagtcggcg gagtagttgg ggcgatttgg
      421 gtgagggtga gaaatccttc gtagggggac cacccacagg atgcgccgag atgctcgcgc
      481 gacaggcgcc caggaggggt ctgaggaatc agggccgcag cgcggttgtc gctgtacaac
      541 ggccggcaca cgtgagggcc gacggtgatc acgcgggtgg acactcaggc gaggccaaac
      601 tgattgtcgg tcggcggtgg gatgctcgcg ggtgagatga gccggcacac cacgttcagg
      661 ttctgcctcg acccgacggt cgagcagcgc gaggtgctcg cccggtacgc cggggcgtca
      721 aggttcgcat tcaatcagtg cctgcacatg gtgaaaaccg ctctcaccca acgcaagaat
      781 gaccccgata tacaggtgct gtggacagga tttgatctga tcaacgcgtt caacgcgtgg
      841 aagaaaaccg aggacgcggg ccgagtgttc accgtcaaca cccgtggcga agctgagatc
      901 gtggtcaccg acctgcgctg gtgctctgag gtgtgtcagc aggtgttcga ggaagccgct
      961 gtggattgtg gtcgcgcttt ggccgcctgg tcggactcgc gatccgaagg caagggcaac
     1021 agggtcggct tcccgcgctt caagaagaaa accagcgcga caccgtcgtt taggctgcgc
     1081 aataaattca gaaagagtcg ccggccggcg attcgagtcg gcgacaacga ccgctcgcgc
     1141 tcgatcaccc ttcctggcat cggacagatc ggggtgcata acgacacccg ccgattgcgg
     1201 cgcatgattt ccaacggcag ggccaagatc ctgttcgcca ccgtgaccca ccacggcggg
     1261 cgctggtgga tatcgctgaa tgtagcggcc gctgagctgc acgccgcaca cctccatcca
     1321 gtccgcgatc ctggagacgc tggcgggtgg gtcggtatcg accgtggatt gtcagcgttt
     1381 gcggtcgccg ccaccagaga cggcgaggag gtcgctcgca tcacctatgc ccccaaggcg
     1441 ctgaatgccg ggatgaaacg gcagcggcgg ctggcgaaat cgttgtcgcg caaacagaaa
     1501 ggatcccgta accgccgtgg cgccgcagcc aagctcgggc gccatcacaa tcacgttgcc
     1561 aatgtgcgcc gccatttctt gcaccaggtg tccaacgagt tggtcaagac ccacgaccgg
     1621 ctcgtgatcg aaaacctgaa cgtgtccggg atgctggcca accatcgcct ggcccgtgcc
     1681 atctccgatg ccggctgggc cgaattcgcc cgccaactaa gctacaagca ggcctggcgc
     1741 ggcggcgaag tcgtgattgc ggatcggtgg tacccgtcga gcaagctttg tcccgcctgc
     1801 ggtgccatcc atgtcaatct gatgctcgcc gaccgtgtgt tcacctgtgg ctgcggacgt
     1861 tccgctgacc gcgatctgaa cgccgcggtg aacctcgcgc gctggggcca acagcatcac
     1921 ctcgatccgc ggaccccgaa gcaacggggc cgggccacca acgcccgccg acgggacggc
     1981 gctgaccagc accccacgcg tgccggtgaa accagcccgg ttgaagcggg aactgacgtt
     2041 cacaccgcac cagcggcctg aaccgatgac gcccgagaag ggcggtgccg acactcgcca
     2101 gagtgttcga cacgctttag agcgtggacc cgcccgcgtc gtggttagag ggggtgtccg
     2161 ttcgcatacg ccgtcgccca gtcccgcacg gtgcgggcgt actggtcgga gtggttgtag
     2221 gcgtgcagcg cgtccatcca gccgcggggc ttggccaggt ccttgccgcg ccagcacagg
     2281 tagcccgccg ccgacagcgc tgcgtcgtcg aaattgtccg ggctgatgat gccgtcgttg
     2341 ttggcgtcga cgccatagag cttccacgtc tcagggatga actgcatcgg acccatggcc
     2401 ctggcgtact gcggatcacc gtctgcgtcg ccgtcgttgt cgatgatctc caggttgccg
     2461 ttggtgccgt ccagtcggac gccgcggatg ggcggcgtga cgtcgccctt aggcccgatc
     2521 accgcgccgc ggtaagtgcc gtggtggctc tccacctgcc cgatgcccgc cagcgtcgtc
     2581 cacgcgatgt ggcagtcggg gttctccacc tccgcgacgc gggcggcgta ggcgtaggcc
     2641 tccagcgcgg cgactggcat gcccagagca ggagcccgct gggccgccca ttcgtgcagc
     2701 tgatcggcgg gcctgccctt ggcgtgggta tcgatcttgg ggaccgggtc ccctgcgggc
     2761 ggcggcaccc cctcgggaat cggcgtgccc agttgccacg agcagctgga agccatcagc
     2821 agcgctgtcg cgcctactac cgcgacagcc cgcagccaac gcactggcga caccggactc
     2881 cctcaaaccc actctggtcg ttgcccaatc gtcccacgcg ttcagaccag atcggcaatc
     2941 gtcgccggac gtgtaccttc ccgagttgcc ccctgctaag gtgaggcaca ccttgctttg
     3001 ggcagccaaa gcaaagtgcg cgggatacgg cgtagcgagg acaattcgat gactgctggt
     3061 acgggcgttg gtgtcagtat tcaggccgcc gcacccaaca tcacgtctca gctgttcggc
     3121 agcgggttga tcggcctgcg cgagggtctc gaggccgcca tcgtcgtcac gatcctggtg
     3181 gcatttctcg tcaaatccga gcaccgcgat gcgctcaagt gggtgtggct gggagtcgcc
     3241 gcagcaatcc tgatgacggt cgcggttttt ctggtgatcc aattcggcga aaacacgatt
     3301 tcgggcctgg cggcggaggc catcgccggc atcgcctcgc tcgtggcggt cgccatcgtc
     3361 acaacgatgg tcatctggat gaagaaggcc gccgcgtcga tgtcgggtga gctgcgcagc
     3421 gagatggccc gcgccctgga aaccggctcg ctggccgtgc tggcactggc gttcttcgcg
     3481 gtcggccgtg aaggcgtcga gaccgccttg ttcatggtcg gctacgcaga ggcccagacg
     3541 tcatggccac tcatcggcct cgtgatcgga gtcttggccg ccgccacgat cgcctacggc
     3601 atgtacgccg gcgccgtccg catcaatctc gccaagttct tcaaatacac cggcgtgttc
     3661 ttgatcgtcg tcgccgcggg aatcctgtcg tacggtgtgg gcgccctgca gacggtcggc
     3721 tggctacctg gcctgagcgc caaggcattc gacatcagta catggttcaa ctggtcgtcc
     3781 tggtacggcg aggtcgtcca gggcgtcttc aacgtcacgc ccaccccgac cgtgctgcaa
     3841 ctcgccgcct ggctggccta tctcgtcatc gtgctgacga tcttcctgcg gccgacgaaa
     3901 gcccctgcga aacccgatcc ttcaccacaa ccccttcccg aatccgaaag gtcgaccacg
     3961 tgaagcttta ttcgtccctc gccgttgcga cggctgcgct ggcggggctt gcactaacga
     4021 gctgccaggc caaggaggag gcaaagcccg ccgatccggg caagagcgcc ccctccgaaa
     4081 tcacggtcac cgcatccgac tcgacgtgcg aactctcggg cactgaagca tcaacggggc
     4141 caagcatatt cgtcatcacc aacaacggcg acaaggtcac cgagttctat gtgtacggcg
     4201 agggcgaacg ggtgatgggc gaggtcgaga acatctcccc cggactgcag cgcaagctca
     4261 tcgtgcagct tgccgagccc ggcacctacc agaccgcgtg caagccgggc atggtcggcg
     4321 acggcatccg cggcgacttc aaggtcaccg gtgacgccgt aaaggtcgat accgagggca
     4381 agttcaaaga agcgtcggac agctacaagc gctacgtgaa cagccagacc gacgcgctga
     4441 tcgccgccac tgcggtgttc gtcgatgcgg tcaagaaggg cgacgtcaac gcggccaagg
     4501 cgcagtaccc gatcgcgcgc acctactacg agcgcatcga gccggtggcc gagtcgttcc
     4561 ccaacgatct cgatccccgc atcgacctgc gcgaggcgga cttggagccg ggccagaagt
     4621 ggacgggctt ccaccggttg gagaaggacc tgtgggtcac cggcctgcag cccgacagca
     4681 atgcgatggc cgaccagctg atggccgacg tgaaggagct cgacaacggc gtgaaggcgc
     4741 ccgagtacaa gatcgactcg acccagatcg ccggtggcgc acagggtctg ctcgacgagg
     4801 tctcctccag caagatcacc ggtgaagagg acatcttcag ccacaccgac ctgtgggatt
     4861 tcaaggccaa cgtcgagggg tcgcagacgg cggtcgcgtc ggtgcggccg atcctcgacg
     4921 agcgcaacgc cgagctcggc aagaaggtgg atcagcggtt cgccgaggtt gaggcgctgc
     4981 tcgaaaagta ccgtcagggc gacggtttca tttcctacga caaggtgacc gagccgcagc
     5041 gccaagagct gtcgcgggcc atcgatgcgt tgtccaagga ggtaagccag gtgcaaggtg
     5101 tcatcgctcc ccaatgaccc gacgccgccg gacgagcggc cgggactttc acgacggaaa
     5161 ctcttcggcg cggctggggt gacggcggcg gtcgtcggcg ccgcgagcgc tggtaccctc
     5221 gccggccggg cgtcggcggc cagcactgac ttcggcgggc tggacaaacc gatcccgttc
     5281 cggggcgagc gtcaggcggg catcatcacg ccgcagcagg accgcatgca cttctgcgcg
     5341 ttcgacgtta cgacggacag ccgtgacgac gtcatcgccc tgctgaagca gtggaccgag
     5401 atggccgagc ggatgacgca gggcgaggat gcggtgccga acggtgcgat cgggggcaat
     5461 ccctatgcgc cgccggacga tacaggtgag gcgctgggac taccggcgtc cgggctgacg
     5521 ctgaccatcg ggttcgggcc gtcgttcttc gagaaggacg gtaaggaccg gttcggtatc
     5581 gcgaacaagc ggcctgccgc gttggccgac ctgccgaagt tcaccaacga gatcatggac
     5641 ccggccaagt gtggcggcga catctgcatc caggcgtgcg cgcatgaccc gcaggtggcc
     5701 gtgcacgctg tccgcaacct ggcccgcgtc gggttcggca ccgtcgcggt gaaatattcg
     5761 caactgggct tcggacgcac gtcgtcgacc acgcgcgaac agctgacgcc gcgaaacatg
     5821 ctcgggttca aggacggaac caacaacatc aaggccgagg accacgacgc gctggacaag
     5881 aacgtctggg tggctgccgg cgacggtccc gagtggctga cgggcggcac ctatctagtg
     5941 acgcggcgca tccgcaatcg catcgaggcg tgggaccgta ccactctgaa ggagcaggag
     6001 cgggtcatcg gccgccagaa gggcagcggt gcacccaacg gctacgacga cgagttcgcg
     6061 ccggtggact tcaacatcaa ggacgccgac ggaatcccac tcgtcgatgt cgacgcccat
     6121 atccggctgg tgtcgcccga tcacctcaac gggatcaaga tgttgcggcg cgggtacaac
     6181 ttcaccgacg gctccgacgg gttcgggcac ctcgatgccg ggctgttctt cattgcgttc
     6241 gtgcggagcc ctgcgacgaa cttcatcccg gtgctggcgc agatgtccgg cgacgcgctc
     6301 aacgagtaca tcttccacac cggcacagcc gttttcgcat gcccgccggg gattcgcgag
     6361 ggcgacaagt cggcctactg gggttcgacg ctgttcgcct gagtttcggg gctccggcac
     6421 taggcggtct gtgcccctcg ttttcgcgaa actgcacttg tcgcgcgact cacttcgcac
     6481 tttcccgcaa caactgcagt ctcgcggagg cccaatatgc ggttagtgct gtcgggtctc
     6541 aaagttcgtg gcgaaatctc acgcgaaatg acgaaccagc agcactgatc ggttggagtc
     6601 gccaagcaat ggatggtagt ccggccagct gagtcgggcg acgtgttcgg cgtcggcact
     6661 gccaaccacg gctaattgga ttgagtcgct gtggttctcg atgggccgcg tccctccccg
     6721 ccgcgcggcg acggtttgca cgtgaacgtc cggatagcgt cgtatccagc tatcgagtcg
     6781 cgtgtcgatc aggaggaccg gcgccttgcg tagccggcct tcctccatcg ctcggtgcac
     6841 gacggcgtcg ttatcaggct cgtcgtttct tacggcggcg atccaaccgg ggcccgtctg
     6901 gggtgcgccg ccggaccgga cgatcgctac ggggcagtgc gcatgcttga caagggcggc
     6961 cgcggtcgcg ccgagtggca tgcgcgcaca cggtcccttt ccctctgatc cgacgcacac
     7021 cagcgcagcc ctgcgggatt catcggtgag cacgcaatcg gggcgcccgc gcacgatggc
     7081 ggtttcgatt tggatcggct ttcccatgtc gtggacggtc atctctgcgc ggtatagagc
     7141 cgattcggcg tgttcggcgc cggaggaaac ggcatcaact cgcgagcggc acgtatgttt
     7201 cgcgacgctg acgtatacga ggcgcagcgg cacaccgagg ttgacggcct cggctaccgc
     7261 ccaggtggcg gcgtcgagtg cggcttggga gccgtcgatg ccaacgacca ccgactgtga
     7321 tggggtctgc tttgtcatgt ccatggtcgc gacttcgatc gtttcgctgg gtgcggcgag
     7381 ggttttagtg gagcgcgacg ggcacggtca tcgtcagcac gaccagcatc agcactagta
     7441 ggaaccagcc ggcgccctgc cagatccacc agcgaccgca tgcccgccag atctgatagg
     7501 tacggacgaa cgccgcgagg ccaccgagca gcaggatcag cggggagccg atccccagta
     7561 ggttgcgttg aacgccaccg cattgcgcta gcgcctcgtc accggtccct gacttgcagc
     7621 cgctcaccca cagcagtccc aacgccagga ccagcattgc gacagccacg atgacgccgg
     7681 cgaagcggag ggcggcagtg aacgtccgct tcgcttgctc tccatgcgag ccggacgaca
     7741 tggttggagt gttcatagtt ggatgcttca cctttgcgcg ggaacggtgt tcaagtatcc
     7801 gccatgcgtg aaactcgggc gccggtgcga attcgactgc cgcgcagtcg gtatgcctac
     7861 cttcgcggta ctgtcttcta cccgcatcat cgggaatgtg gtgtcgtcat tgccgctgca
     7921 ggaatcgctg gccgccatgt ggtctccttt cgcgttccaa cccgtggggg tggtgatgat
     7981 ttcggaaact attgattgag ccggaagggc tggcccgcaa cggtttccaa gggcagactg
     8041 cgagggtcgt cggggatcgg cggggcgtcg acatgcggcg tctcgccgtt gggctgcgcg
     8101 gcgggttcgg cgggcgtcct gcctgctgtt tctggggccc cggatggttg tggcgtggct
     8161 ttttccaaga accgctgcag ctccacgggg aagggaagca ccagggtgga gtcaacttcg
     8221 tcgtaaagcc gctgcatcgt cacgtctagt cgatgtgcgg cgtcggcggc gtcgatcagg
     8281 tcgttgagca gtcggggtgg cacggtgtgc tcgtgcttgt ggtggatctt gtgctccagg
     8341 ttggcccaca agtccatcgc gatcgttctg atctgtagtt cgaccggcac gcgctcgacg
     8401 cggtcgccca ggtagacggg gatctcaagg accatgtgaa agcttttgta gccgttgggc
     8461 ttcgggtggg cgatgtaatc ctcaacctcg atcacggtga ggtcggcctg ctggctgagc
     8521 aaggtggcga cccggtaggt ctctgaaatg aggccgcagg tgacgcggac cccggcgata
     8581 tcgaggacgt tggtgccgat gtcgtcggcg gtgagcgggc agccgatgcg ggcggcctta
     8641 cccaggatgc tgttgggcga tttcacccgg gaggtgacgg cgcggatcgg acagtagtga
     8701 tcggcgtgag tgagctcgcg cttgagaatg ttgaccttgg atgtcagtgc ggaggtagcg
     8761 cactgatacg gcagcaggaa ccgggcgaag tcggtgtgcg caggcgtgaa ctcggtctgc
     8821 gataggtcgc cgaccacgct gtgggcacca tcgacgatca tcttcgcctc ttcttctcgc
     8881 aacactgcta atataaagga agtatatccg ttaaatataa gaagggtaag ggctggtggg
     8941 cgaagaagca gattccaagg ccgggcgcag ccggcgagat cgcctgccgc gcaaccggca
     9001 acgtgaacga gtgcttcaga tggtgcgtgc gcacggcgac gcgattgacg ctgtcgagct
     9061 cgcctcgcgg atggggttgc acgtcaccac cgtgcgcttc catctggacg cgttgtgcga
     9121 cgagggaacg atcgagcgca cccgaatgaa ccgggacggc gtgggtcgtc cacgcaccgg
     9181 ataccgcgcc gtcgaggagc gtctggacta tcggatactc gccgagattc tcgcgatgga
     9241 gctcggagag acagtggaaa cacgcgctcg tcgtgcccag cgcgtaggag tgcagtgggc
     9301 cggccggatc gcaggttcgc aggccgaacc tgttgcgggc caacgtgttg cggataccga
     9361 gcaggcgcta gacccgctgg atcgaggagc ggtgttggcc accgaggtct tcgaccggat
     9421 ggggttcgac cccgaactgg ctgctgaatc cgaaccaatg gcatcgctgt cggctgacag
     9481 caagcagatc gtcggacgcg aacgggtcat tcggctgcgc ggctgtccgg tacgggattt
     9541 ggcccgagcg caccccgagg tgggttgcgg catacacctg ggattgctgc aaggcttact
     9601 cgaccatgcc gcggcggccg gtggaaagcc cgacacgcga acgcaaggac tgtcggccag
     9661 actcgacccg ttcgtcgagc cagaattgtg catagccagg ttgcacgcct cgcgaaaatc
     9721 cacgcgtgat tgaggcatgg catgtgagaa cccgctcacg acaggcgatg acggacttgc
     9781 gccgtccagc gattgcgctg taacgcatcc gcccacagtg gccccagacc aacgcggccg
     9841 gggactgcgt aaccggcggt gtcgcgggac ccgcgtgcaa ggtgtaggtc gctgtggtag
     9901 acgggctagc cggtcagttc tgtcaacggc acgtcggggt cggccagttt cgatgggtcg
     9961 ggttgcgcgg cggagcgcac cagcgcctcg atggcgtcgg tgacatccca tgtgttgacg
    10021 ttcatgccgg ccagcaccgt tccgcccttc atccagaagg cgatgtactt gccgctggcg
    10081 acgtcgccgc ggaagacgac ctcgtcgtag ccgccacgcg gtacgtagcc ggaatactcc
    10141 atgccactgt cgtattggtc ggagaagaag tagggcacct tgtcgtaggc ggtgggcgcg
    10201 cccatcatgt tcgccgctgc gaccggccct tggttgaggg cggccgacca gtgctcgaga
    10261 cgcaggtgag tgcccagcag cgggtagtag gcgttggcga catcgccggc ggcgaatata
    10321 tccgggtcga tggtggccag gtgctcgtcg acgacgacgc cgttgtcgat cggcagacct
    10381 gcggccgcgg ccaactcgat attgggcagg atgccgacgc cgatcaagat cgtgtcggcc
    10441 gccacgacgt ctccgtcggc caggcgcacg ccggtcacct tgtcggcgac gccgaggatc
    10501 tcggccaccc cgctgccggg ccgcagctcg acaccgtggg cgcgatgcag ggccgcgtaa
    10561 gtctcggcca cctccgcgcc gaggacaccc agtaggggca gcttgcccct ctcaaggaga
    10621 gttacgtgac agccggcggc gcgggcggcg actgcggtct ccaagccgat ccacccggcc
    10681 ccgatgatgg ccacccgctg cgcagatgcg aacgcggcct gcaatgcgtc gctgtcggcc
    10741 accgtgcgca ggtaatgcaa accgggcagg tcggcaccgg gtacctcgag gcgccggggc
    10801 gaagaacccg tcgccagcag cagcttgtcg tagccgagcg tttctccctg cgctgtgcgc
    10861 acttcgtgtg cagcgcggtc gatttcggtc acccgtgtgc ccaaacgcag gtcgatgtcg
    10921 tgttcggtgt accactgcgc gggatggatg tagatctttt cccgctcggt gctgcccagc
    10981 aggtagccct tcgacagggg cgggcggtca tagggacggt tggcctcgtc gccgatgagg
    11041 atgaccttgc cgtcgaaacc gttggtgcgc agcgcctcag cggccttggc gccggccagt
    11101 ccggctccca cgatgacaaa tgtctgctgt gcactcacgt gcgtctcctc ctcgttgtgt
    11161 gggcggcatc gtgttcacgt tgcgacccgg ccgaatcctg ttgcggcgct tgcctctcgc
    11221 agcctcgtcg tagcgcatct ggtcgactgg ccgagtggtg cgcagatcgt ctggacgcgt
    11281 ttggcggggg tcgccgcata ggtgatcatt gctgcgctag ctggtgggct ttgtcgcgca
    11341 atgcgatcag cagatcggcg aacgactggt gaaagccggc gacgccttgg tcttcgaggc
    11401 tgcgcccgac atcgtccatg tcgatcccca ccgagcgcag gttttgcatg acgtcggtcg
    11461 cctcccccat cccggtgtcg atggtgcgcg cgacggtgcc gtggtcctcg aacgcggcga
    11521 tggtggcctc ggccatcgtg ttgatggtgt cggggccgat gaggctgtcg acgtagaggg
    11581 tgtcggggta gtcggggttc ttggtcgacg tcgatgccca caacggcgat tgcgggtggg
    11641 cgccgagggc ggccagtttc tcccaccgcg gaccggagaa tcggtcccgg aacaattggt
    11701 aggccaggcg tgcctgcgcg atgccggcgc gtccgcgcag cgccgccgcg tccgcgccgc
    11761 cgatctgctc caaccggttg tcgacctggg tgtcgacgcg gctgacgaag aacgacgcca
    11821 cactgcggac cgccgggaga acacccccgc gtgcggcaag ggtttccagg ccggctaggt
    11881 aggcctcgat gacctgctcg tagcgggaca gggagaagat cagggtgacg ttgatgctca
    11941 acccgctgcc gatcgtcgct gcgatcgccg ggatgccctc ggcggtggca gggatcttca
    12001 ccagcaagtt ggggcgggcg atgcgctcgt gtaatcgggt ggccgcggcg atcgtggccg
    12061 tggtgtctcg cgccagctcg ggggcgactt cgatggatac gaaaccatcg ctgccgtggg
    12121 agttgtcgta ggtcggacgc agcaccgcca gggcgtcggt gacgtcgtgc acggccaatt
    12181 cccaataggc atcctcgatc gaatagcccg cggcggtgag ggcgaaaaac tgtgcgtcat
    12241 agagatcgga ggccccgatg gccttggcca agatcgtggg attggctgtc accccgcgta
    12301 tcccgtcggc cacgtaggcg gccagtgtgc cgtcgcgtag gtataggcgg gtgaggttgt
    12361 ccagccacgc gctttgccgc tgctcggtgt agagccgctc gagcttgatc atcacgcaca
    12421 cccaccccgg tcgggtgacg cggtcatcgt gtcgtgtgcg agggccggcg acatgatcaa
    12481 cgcagtcatg gtgctggccg gtcaggggtt tcgctgtcgg cggggacggc tggatgaggt
    12541 cggcgtcgcc gacgcgtgcg cgccgacggc tgcgcgtcgt gcaccaccac acagcaccgg
    12601 tcaggcgccg gatcgagctc ggcgtgcgca tgcgggtgac cacaccctgc gatgaccccc
    12661 tcgacgaggc gtaagttcag cccgcacacc acgtctcggt ggtcggcagc gaccaaatga
    12721 aacgggcaat tccgtagatc gatgcggcca tcgtcatcgg cgtggggcag gtagccgcag
    12781 ttgcgaagcg cgcccaccac gcttcccccg gtttccgcac cggcacgccg gccggcctca
    12841 tgcgctgcgc tgttcacggc ctgctgcacc gctccgctgg cgtcctgctc aacggatgcg
    12901 acgagtaggc gagcgaggag ttcgtaatcc cggggcggca cgctgacggt gatctcctgg
    12961 gcggcgcggg tgtagagctt ggccgggcgc ccggcccccg gtcccccacg cccagcgggc
    13021 cgttggtagc tgacggtcag cagtcccgcc tcggccaact tgtccaggtg gtaggccgcc
    13081 agcgtgcgtc cgatcgacgc ggcggcggcg gcctgctcac gcgaaaccgg tccgtcgctc
    13141 tcgatcacgt actcgtacaa ctgccgccgc agcgggtcgt ccaggtgact cagaccgttc
    13201 agtgcggcca ggtcggcggg gcggctgatg gacatacgcc aactctaaca ccaaaaagta
    13261 ttgacaaata ggcgtcagcg gcagattcta aaactagttc tgattggtct tacaaaggag
    13321 attgcgatga ccgcttcagc gctacccgcc tcagccgccc acgcctcctc cggggtgtta
    13381 cagcgcgccc gctccgatcc ggcctacgca gcgttcctgt tgctccggat cggattcacg
    13441 gtgttgccga tcgtgtttgg cctcgacaaa ttcacgaacg tgctcaccaa gtgggaggtc
    13501 tacctggctc cgtggatcgt ggacatgagc ccgatcagcg cgcaccaggt gatgctggtg
    13561 gtcggggtaa tcgagatcgt ggccggcgtg gccgtggcga tcaagccgcg atacgccgcc
    13621 tacatcgtcg cggcctggct ggccggcatc atcgtcaacc tgctgagcta ccccggcttc
    13681 tacgacgtgg cgctgcgcga cgtcggcttg ctgctagccg cgctcaccct cgctcggctg
    13741 gcctttgtgt acgacccggc ctggcaccgg cgcaccacag cgggcaccga ttcccctgcc
    13801 gcgtagtcga cctcacgtgc tgccctccgt tcatccggcg ccacctacgc ctgcgacggg
    13861 tgaacggagg gatcctctcg tctccggaag gaacccgcca tgcattccac cgccgctctg
    13921 cggctcgtgc acgaatcacc ccatcgcgat cttgccgccg cggaagtcgc cgccgccgcc
    13981 ttccttcgtg ccctgggggt ttcactggat tcgcagagcc ttgccggcac acccgcgcgg
    14041 atggcgcgcg gctacgccga gctgttcaca ccgcggacct tcgatctgac caccttcccc
    14101 aacgacgagg gctacgacga actcgtgatc tcgcgcaacc tgccgctgcg atcggtgtgc
    14161 gagcaccaca tgttgccctt tgtcgggatc gcccacatcg gctatctgcc cggcgagcgc
    14221 atcatcgggc tatccaaact cgcccgtgtc gccgagcatt tcgcctgccg gcctcaacgc
    14281 caggaacgac tcaccaaaca gatcgccgat tggctcgacg cacagctgca gccccgcggt
    14341 gtcggcgtgg tcatcgaggc cgagcacacc tgcatgaccc tgcgaggcgt gcaggccgcc
    14401 aggtcggcga ccatcacctc cacccttttg ggcgcgctgc gcgaagaccc gcgatcacgc
    14461 caggagttct tcgcactcac cggcgccaac acctgaccaa caacgaacac caacccaggg
    14521 agatgtcatg actcagcgac gaaaagtcaa ggcgcgccgg atctatgacc cggccctgcc
    14581 gcaggatggc acccgggtcc tcgtcgatcg catctggccc cgcggtgtga gtaaagcccg
    14641 cgcacagctc gacgaatggt gcaaggacat cgctccgtcc accgaactac gcaagtggta
    14701 cagccacgac cccgcgctgt tcgaggagtt cgctcaccgc taccgcgacg agctgagcga
    14761 gcccgcccac gcggccgcat tcgcgcatct gcgcgatttg gcgaaggcag ggacgctaac
    14821 cctgctcacc gccaccaaga accccgacct gagcgaggcc gcggtcctcg ccgatctggt
    14881 cggttcgggc caacgccggc ccgtcggctg aaaccccaca tcccaccggc ggttcagccc
    14941 cgtggatgtg tgacatcgag gacccggccg cctcaatgac tatgcaccgg tgaggcattc
    15001 acgtcgcgtg tggcgcttgc tcacgtcgca gcacgtacca ctccgcgcag ccgcgacgcg
    15061 atcagctcgc gtgggagccg gctcgggctg ggataggcga cgatgacccg gccgtggtcg
    15121 gggtcgcagc cctccacgaa cgtcaacccg ttctcggtgt atgtgtaggt ggccggatcg
    15181 tgagctcgca gacgattggt cacccgctcc aactcggctt ggctatcggt ggcccacatc
    15241 aggtattcga tcccgatagg cgccagccgc ggccgccgag acgggcccgt cgaatgcaaa
    15301 tagatctgaa acccgtccgg ggtcaacagc agcgctgtgt cgggttcgcg cagggcgacc
    15361 cggcagcaga acacatcgcg atagaagctc accgatcggt ccaagtcagc tacccgtatc
    15421 actgtcgacg cgaccgtcgt tgtgacgcca ccgatcgggg ctggttcagt cgcctgcgtc
    15481 atggcctccg ccccaatcta tttagagtaa aatgctcctc tataatagca ctgcacgcgt
    15541 tcagccggaa cgactgagtt gacgtgacaa ccgcgacctt cggtccatca gcgacgacaa
    15601 cttacgagtg gttaattcct tgcggacagc ggcaattcga ctcgacaccg atattgcccc
    15661 cctgacgttt cgtcgatccc ggcggaagtg cacggtgcct ctgctccgcc ccggcggccg
    15721 acggcgcgcg gtggggtccg caatgtcttc gtcaaatgaa atctcccgtc gcaaaaaaag
    15781 cgtgacgtgc tggccgtgct catccgccgg aggggttgct cgtgggtgcg gccggcgttg
    15841 cgcgttctgc ggccgggttg gagtgttggt ggcggccgac gtgggcttcg gcgcgcatcc
    15901 ggtcggccat gtgcgggtag tgcagttcga aagcgggccg ctccgagcgg atccggggca
    15961 gttcggtgaa gttgtgccgt ggcggcgggc aggtggtggc ccattccagg gagttgccat
    16021 atccccacgg gtcatcgacg atgacgacct cgccgtagcg gtagctctta aagatgttcc
    16081 acacgaacgg cagcatggac gccccaaggg tgaacgcccc gatcgtcgac acgatgttca
    16141 acgtggtgaa tccgtcggcg ggtaggtagt cggcgtagcg gcgcggcatg ccttcgttgc
    16201 ccagccagtg ctgcaccaaa aaggtggtgt ggaaaccgat gaacgtcagc cagaagtgca
    16261 gcttgcccag ccgctcgtcg agcagccggc cggtcatctt cgggaaccag aagtagatgc
    16321 cggcataggt ggcgaacacg atggtgccga acagcacgta atggaagtgt gcgaccagga
    16381 aataggtgtc ggtgacgtgg aagtcgatcg gcgggctggc cagcagcacc cccgacagac
    16441 cgcccagcag gaaggtgacc atgaagccca ccgagaacag catcggtgtc tcaaatgtca
    16501 actgcccgtg ccacattgtg ccgatccagt tgaagaactt gatcccggtc gggatggcga
    16561 tcagatacga catgaatgag aagaacggca gaaggacggc gccgggggcg aacatgtggt
    16621 gcgcccacac cgcggtcgac agcgcggcga tgctcacggt cgcgtacacc atggtggtgt
    16681 aaccgaagac cggtttgcgg ctgaacaccg gaacgatctc ggtgatgatg ccgaagaacg
    16741 ggatcgcgac gatgtacacc tcggggtggc cgaagaacca gaacaggtgc tgccacagga
    16801 tcactccgcc gttggcgggg tcatagatgt gaccgcccag gtgccggtcg aacgccaacc
    16861 ccagcagcgc ggccgtcagc agcgggaacg cgatcagcat catgacgctg gtgaccagga
    16921 tgttccaggt gaagatcggc atccgaaaca tcgtcatccc cggcgcacgc atacagacca
    16981 cggtggtgat catgttgacc gcgcccagga tggtgcccag accggcgatg gccagcccca
    17041 tgatccacag atcggcgcca gcgccgggcg aatgcagcgc gtcgctgagc ggagtgtagg
    17101 cggtccaccc gaagtcggcc gcgccccccg gggtgaggaa accaccgacg gtgaccagcg
    17161 cgccaaacag gtacagccaa taactgagcg cgttgagccg cgggaacgcc acgtctggcg
    17221 cgccgatctg aagcggcaga atgcaattgg cgaaaccaaa cacaatcggg gtggcataca
    17281 gcaacagcat gatcgtgccg tgcatggtga acagctggtt gtactgctca ttggacaaga
    17341 actgcaaccc cggcgcgccc agctccgagc gcatcagcag agccatcaac ccgccggcca
    17401 tgaagaagcc gacagaagtg accgtgtaca tgatgcccag cagtttgggg tcggtggtcg
    17461 tgagcacccg gtacacaaac gatcccttga ctccgcgccg cggcgggaac ggccgggtcg
    17521 gtgtcagttc gacgacagca ctcacggtgt gcccagatcg gcgcggggat gggctatctc
    17581 gtgcgccatg tgcatcacca cttcccctta cccaaatcct atttaactga ctatagcccg
    17641 ttaaatagga tcggggtaag ggcgcaggca ctagcgcccc gcggcatgcg aggccgctgc
    17701 cgcctagcgc actgcttcac ccatcggcgc gttaacaatc ggcgcgtcaa ggccgttcga
    17761 acgcgaacac tccgtcggca acgtcgtcac agggtttctc caacgtcgat gacgtggtgt
    17821 cagagcaggc cggcgaggtc gggttcggtg attcggacga tccggatccg ccacagtctg
    17881 tcggattggc tgccctccag atagcgccag tcatacgcgc cggggcggtc gcgggcgaac
    17941 tcctcgcgca ggtgtatcgg gtcgtggctg ttgacgagta cgaacgactc gccggcagcc
    18001 agtgcgtcga agcgggcgaa gatcaacggg tgccgttggg atttgggaat ctggcgcacg
    18061 tcgagctcgt cggcgtcatc ggctgtatgc ggatcgtaga gagtcatgag cacatactat
    18121 tcctacaagt ggtaattgga gtaatgtcgt gggcatgaca tacgtgatcg gagcgccgtg
    18181 cgtggacatc aaggaccgtg cctgcgtgga tgagtgcccg gtggactgca tctatgaggg
    18241 cgcacggatg ctttacatcc accccgacga gtgtgtcgac tgcggcgcgt gcgaaccggt
    18301 atgcccggtg gaggcgatct actacgaaga cgacctgcct gagccgctgc ggccctacat
    18361 cgatgagaac gtcaagttct tcaccgacac tctgccgggc cagaccgatc ccctgggatc
    18421 accgggggga gccgcgaaac tcggtgccgt cgatgccgat accccgatgg tggcggctct
    18481 gccgccgcag ggcgactgat gcccaccgac gcaaccgcgc ccgcaccggg gatgggtccc
    18541 ccgggccgcc gcgacgtcat cctggcgctg ctgcgggcct cggcccagcc gcgcagcatc
    18601 gccagcgttg ccgacgaact cggcgtgcac ccgaacacgg ttcgctttca catcgacgcg
    18661 ctgctgcgca ccggccgcgt tgaacaagtc ctcggtgact ccgccggccg cggccgtccc
    18721 ccgatcctgt ttcgcgccag ccgccggatg gacccgacag ggccgacgaa ctatcggctg
    18781 ctcgccgcca ttctgactgg ctatgtggcc cgggaacccg atgccgccgg catcgccgcc
    18841 cagttgggcc gttcgacgag tccggcgctg gtggcgtcct ccggccagcg ccgggcgccc
    18901 tcgaaaaccc gcgcagtcac cgaactggtg gagttgttgg cggagttggg ttttgagccc
    18961 gagcccgccg agggtccgcg cacgcgcgag atccggttgc ggcactgtcc tttccagaac
    19021 ttggccgaac aacatggcga ggtgatctgt tctgtgcacc tgggcctgat gcagggcgcg
    19081 ttgagcgcga tgcgggcacc ggtcaccgtg gatcgtctcg agccgttcgt cgagcccgac
    19141 ctgtgcgtgg cgcacctggc gcccgcaccg tcccgtgatc gggcatcgtg accgagctcg
    19201 ctatccgggt ggctgccgcg gcgacgctga tctggctggg catggtgttg gcgatctctt
    19261 tcctggaggc accactgaag tttcgcgccg agggcctcga gttacgggtg ggcctggcca
    19321 tcgggcgtat cgtgttccgg gccctcaaca tcgtcgaggt catctgggcg gtggtcatcg
    19381 cggtatgcct gagcgtcggc ggaccatccg gcccggttct cagcctcgct gcagtaacgg
    19441 cggtactgct cgccgtgcag
//
LOCUS       DASRJA010000346         8137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3669952,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000346 DASRJA010000000
VERSION     DASRJA010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8137
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3669952"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFC01_15370"
     CDS             <1..249
                     /locus_tag="VFC01_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZN81045.1"
                     /translation="QTMLEASGNEMFRALNGVVAELLAGRTHHGMMPDLPNPAAIELH
                     DEVARAIRMRDEEAAERAMRAIIDEATSAVAEEFSEPS"
     gene            complement(246..965)
                     /locus_tag="VFC01_15375"
     CDS             complement(246..965)
                     /locus_tag="VFC01_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HZN81046.1"
                     /translation="MGALATIWALVSGCDASVGIGGPPTVDKKALQTDIANRLAKAGE
                     QPESVRCEQDLVGEVGKTARCEVVMSPTNSFEPVITVTSVDGSTINYEMSPALSKTQL
                     EKAVSRLIADAAHAQFVSVSCESGLDGKVGATAHCDVEAGGVSLRRTVEVNSVEGLMM
                     NFDVVPLLTKEEVASSLLGEVERQLGRRPDSAQCAGNLEGKVGNTVDCTIVTGSDRTS
                     FTLTVTTVSGGSIRYSYAPRP"
     gene            1081..1965
                     /locus_tag="VFC01_15380"
     CDS             1081..1965
                     /locus_tag="VFC01_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81047.1"
                     /translation="MARLPSLARRFFDRFEPVHAVTYFAPEARTALDGLGFRGFWMGY
                     FAARSAPFGVVATEVVTAAFYNFAPERVAKALPAAWDVASPSDVLRLRRDSAVAALRR
                     YGVTDTEAAAAAELAEKAARSAPLDGRPLFAANVALDWPDDPVARLWHAVTLLREQRG
                     DGHVAVLTAFGISGRESNVLHAAAGRVPVEMIMRSRDYDEEQWRHYGERLARRGLLDG
                     DGALTDAGRNLKQRIEDATDQLALSALEALDDDEVERLFQALTPITRKVVAAGDVPAA
                     TPMGLGRDDLHDDSAHLA"
     gene            2108..2914
                     /locus_tag="VFC01_15385"
     CDS             2108..2914
                     /locus_tag="VFC01_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN81048.1"
                     /translation="MRIIVTGGNSGVGKATAAALADSGHSVVIACRSIEKAEQAAADM
                     SGDVEVGQLDLADLASVRAFAESVEPVDVLVNNAGVMGLPLTRTAGGFEAHMGINHLG
                     HFALTCLLGDRIKDRVISVASAMYLFARIHFDDLNWQSRRYSAMTAYAESKLANLLFV
                     RELANRGVRAYAADPGMTDTGITRSAAVGLPVGERNPVWHILQSPAQGARASLQAVTT
                     DLPSGTYLAPRFNQWGKPRVTGLRKKARDPVAARRLWELSVELTGCDWSR"
     gene            complement(3020..4957)
                     /locus_tag="VFC01_15390"
     CDS             complement(3020..4957)
                     /locus_tag="VFC01_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZN81049.1"
                     /translation="MRTTDVVERPADLTAEWLTATLGQSVSDFAVERIGTGQMSECYR
                     VALTYAEGAGGPNSVVLKVAATDPVSRQTGLALGLYEREVRFYTDIAPGIDGPIAPCH
                     HAAFDTETGAFHLLLGDAGPAVVGDEIRGATVEQAMLALSELGRVHAPLLGDTAMANA
                     DWLNRESPMNQAVIGQLYAGFIDRYGDQIAPEHREVCERLVATFDAYVASESDRVPGL
                     VHGDYRLDNMLFGEAGADRPLTVVDWQTVTWGPAMTDVAYFLGCALPDRLRRDHYDAL
                     LRAYHQALGAKLSLDEVRDGVRRQSFFGVLMAIISSMLVAQTSRGDEMFMVMLRRHCQ
                     HVLDTDALAVLPEPVAAVPLTPVADDEGAHDPTDEPLWNESWYFDFTDPGQGVGGWIR
                     LGLMPNEKTAWITALLCGPDMPTIAAVDFHDTGAIELSLEATEPLQACTVSVRGTGQA
                     YDDPAALLRSDSGRPVELTMDLVWTTAGTPYQYRITPRYEIPCTVSGTITVDGREIPL
                     RAVAGQRDHSWGVRDWWAMDWVWSALHLDDGTHLHGVDIRIPGAPPIGVGYLQQTGRP
                     LVELQAVTARETFGDNGLPQTTTIDFGALKATIDIRGHAPVLLTAPDGRISHFPRAWA
                     TVTTDDGRTGVGWIEWNRNQR"
     gene            complement(4944..6875)
                     /locus_tag="VFC01_15395"
     CDS             complement(4944..6875)
                     /locus_tag="VFC01_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HZN81050.1"
                     /translation="MRNPVAHPLDPLDADEFRAVASILQREHGVGDGWRFASVELAEP
                     AKSDLRAFDDGGPRPPRRAEVICLHRSDNATYKSVVSLTDDRVESFEHVPGVQANFTV
                     DEFVECDQLLRANPDLIAALAKRGITDMDLVFFDTWTYGDAVAPPEYRDRRLGWSDTW
                     VKAVPGANPYANLVSGLHCVVDVNSMELLRVEDNGGVDTPEVMGEYVPSRIPERIRSA
                     SRREPLKPLHITQPEGPSFTLDGNLLQWQNWSLRIGFNHREGMTLHTVGYRDGDRNRS
                     VAHRLSFAEMIVPYRDPSDDHYRRTAFDIGEWGLGFMTTSLELGCDCLGEIRYLDAVL
                     HNSKGEPYTITNAICIHEEDNAVLWKHVDHEIGAEVRRMRRLTISFHVTVANYEYLVY
                     WRLYQDGNIECEVRATGIMVTTPLAPGQPHPNGTLVDERTYAPFHQHFLVARLDMDID
                     GSDNTVYMTESHPEPIGRDNPHGLSLVVRNVALRTESQAKQDVNFATQRAWKVVNTNV
                     VNGLGTHPAYKLVPTGAIPPMFDPESPVVERASVIGHTLWVTPNRPDERWPAGEFVNQ
                     SKRDTGLGRWTLANRSIDNTDVVMWYVFGIHHITRPEDWPVMPVDVVSFWLKPFGFFD
                     RNPSLDVPGAHTPTTSAHH"
     gene            complement(6917..>8137)
                     /locus_tag="VFC01_15400"
     CDS             complement(6917..>8137)
                     /locus_tag="VFC01_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HZN81051.1"
                     /translation="AHPLPVMFFIHGGGYFMGSSATPIYDGAALAREGCVYVSVNYRL
                     GALGCLDLSSLSTKDITFDDNLFLRDLVMALRWVRDNIAVFGGDPDNVTIFGESAGAH
                     AVATLLAVPAAKGLFAQAISESTAAGMVRSPEIAAEYATKFAALLGARKEDGADAVLT
                     ARPEDLVNAFDRLVRQGQREMLGAFAAGPTRGTDCLPLDPVEAMRVGAAHRLPLIIGT
                     NAEEARLFGRFLKLLPMTEPMIERLLAAAEPGERERITAAYPGYPDPAACIQFGGDFA
                     FGSAAWQIAEAHSRHAPTYLYRYDYAPRTLRWSGLGATHATELLAVFDVYRTKFGRLL
                     TAAADRRTALRVSDDMRARWHAFSRTGTPGEGWPAYTAADRAVMVFDRRPRVEYDPHA
                     ARRQAWEGFSLATG"
BASE COUNT         1372 a         2700 c         2678 g         1387 t
ORIGIN      
        1 cagacgatgc tcgaagccag tggcaacgag atgtttcggg cgctgaacgg cgtcgtcgcc
       61 gagctcctcg ccgggcgcac ccatcacggc atgatgcccg acctgccgaa cccggcggcc
      121 atcgaactgc acgacgaggt cgcccgcgcg atccggatgc gggacgagga agccgccgag
      181 cgcgccatgc gcgcgatcat cgatgaggcg acatcggcgg tggccgaaga gttttcagag
      241 ccgtcctagg gtcggggcgc gtagctgtac ctgatactgc cgccgctgac cgtggtgacc
      301 gtcagggtga acgatgtcct gtctgaaccg gtgacgatcg tgcagtcgac ggtgttgccc
      361 accttgccct cgaggttgcc tgcgcactgt gcggagtcgg gccgtcgccc aagttgtctc
      421 tcgacctcgc caaggagcga actcgcgact tcctccttgg tgagcagcgg caccacgtcg
      481 aagttcatca tcagaccctc gacgctgttc acttcgaccg tgcgccgcag gctcaccccg
      541 cccgcctcga cgtcacagtg cgcggtcgca ccgaccttgc cgtccaggcc cgattcgcac
      601 gacaccgaca cgaactgcgc gtgggccgcg tcagcgataa gccgtgagac cgccttctcc
      661 agttgagttt tggacagcgc tgggctcatc tcgtagttga tggtgctgcc gtcgacactg
      721 gtgaccgtga tgaccggctc gaagctgttc gtcgggctca tcacgacctc acagcgcgcc
      781 gtcttgccga cctcgccgac cagatcctgt tcgcacctaa ccgattccgg ttgttcgccg
      841 gccttggcca gccgattggc gatgtcagtt tgcagagcct tcttgtcgac ggtaggcggg
      901 ccaccgattc cgacgctcgc atcacagcca gagaccagcg cccatatggt cgccaacgcg
      961 cccacggctg taatctccgc atgcaagggc cgcctcctgg accgttgctg gctcgccgcc
     1021 acccgggcgt ttgcacggac catagcaagt tggctgatga cgtgatggtt aactgcgaaa
     1081 gtggccagac taccttcgct cgcgcgccgc ttctttgatc gcttcgaacc ggtgcacgcc
     1141 gttacgtact tcgcgcccga ggcgcgaacc gcgctcgacg ggctgggatt tcgcggattc
     1201 tggatgggct actttgccgc tcggtccgcc ccgttcgggg tggtggcgac ggaggtcgtc
     1261 accgccgcgt tctacaactt cgcgccagag cgcgtcgcca aggcactgcc cgcggcatgg
     1321 gatgtcgcgt caccatcgga cgtgctgcgt ctccgccggg attcggcagt ggcggcgctt
     1381 cgccgttacg gcgtgaccga caccgaggcg gccgcggcgg ccgagctggc ggagaaggcc
     1441 gcgcgcagcg cgccactcga cggtcggccg ttgttcgcgg cgaatgtcgc gctggactgg
     1501 ccggacgacc ccgtggcccg gctatggcac gcggtcacgc tgctgcgcga acagcgcggc
     1561 gacggacatg tggccgtcct aaccgcgttc ggcatctcgg gccgagagtc caacgtgctg
     1621 cacgcggcgg cgggccgagt tccggtagag atgatcatgc gcagccgtga ctacgacgag
     1681 gagcagtggc ggcactacgg cgagaggctg gcccgtcgcg ggctactcga cggcgacggc
     1741 gcgctcaccg acgctggccg aaacctcaag cagcgcatcg aggacgccac cgatcagctg
     1801 gcgctatcag ctttggaggc actggatgac gacgaagtcg agaggctgtt tcaagcgctg
     1861 acgccaatca cacgtaaagt ggtcgccgcc ggcgatgtgc ccgccgcgac cccgatggga
     1921 ctgggccgcg acgacctgca tgacgacagc gcccatctgg cttgaccacc tgcccgcgaa
     1981 ggtgcgtgtc tgcaccccga cacgccgtat tctgcggaca ttttgcgcac gtcccctact
     2041 tgcgtgtggg cccacgcggg acgctgcggg acgtgctcgc tggattgctc atgtacgctc
     2101 cgcgctgatg cggatcatcg tcacgggcgg caacagcgga gtcgggaagg caaccgcagc
     2161 cgcgctggcc gacagcggcc atagcgtggt gatcgcctgc cggagcatcg agaaggccga
     2221 gcaggcggcc gccgacatgt cgggcgatgt cgaggtggga caactcgatc tcgccgatct
     2281 cgccagtgtg cgtgcatttg cagagtcggt ggaacccgtt gacgtgctgg tgaacaacgc
     2341 aggcgtcatg ggtctgccgc tgacccgtac ggccggcggt ttcgaggcgc acatgggtat
     2401 caaccacctt ggccacttcg ccttgacgtg tctgctcggc gacaggatca aagaccgggt
     2461 catctctgtg gcgagcgcga tgtacctttt tgcccgcatc catttcgacg acctcaattg
     2521 gcagagccgc aggtactccg cgatgacggc atacgcggaa tccaagctgg ccaacctgct
     2581 gttcgtccgc gagctggcca accgtggcgt ccgggcgtat gcggcggatc caggcatgac
     2641 cgataccggt atcacgagaa gcgccgcagt cgggctgccc gtcggcgagc gaaatccggt
     2701 gtggcacatc ctgcaaagtc cggcacaggg ggcgcgggcg agccttcagg cggtgacgac
     2761 cgatctgccc agcgggacgt acctggcgcc gcggttcaac cagtggggca aaccacgagt
     2821 aaccggtttg cgcaagaagg ctcgcgatcc ggtggcggcc cgtcggttgt gggagttgtc
     2881 tgtcgaactg accggttgcg actggtcgcg ctgaccctca ctgcgcgacg agcgtgcgcg
     2941 aaatgtacgc agaatacggc gtgtcggcgt gcagactcgc acgtccccag cttgcgcgtg
     3001 ggcccacgcg gagatggggc taacgctggt tgcggttcca ttcgatccag ccgacacccg
     3061 tgcgaccgtc gtcggtggtg accgtcgccc atgcgcgcgg gaagtggctg atgcgtccgt
     3121 cgggcgccgt cagaagcacc ggtgcgtgcc cgcggatgtc gatagtggcc ttcagcgcgc
     3181 cgaagtcgat cgtcgtcgtc tgcggtaagc cgttgtcgcc gaacgtttcc cgtgccgtga
     3241 ctgcctgcag ttcgaccagc ggccgacccg tttgctgcag atatccgacc ccgatcggcg
     3301 gggcgccggg gatgcggatg tcgacgccgt gaaggtgcgt gccgtcatcg agatgcagtg
     3361 cgctccacac ccagtccatg gcccaccagt cgcgcacacc ccacgagtgg tcgcgttgac
     3421 cggcgaccgc ccgcagcggg atctcccggc catcgacggt gatcgtgccg ctcaccgtgc
     3481 acgggatctc atagcgcgga gtgatccggt actgatacgg cgtgccggcc gtcgtccaca
     3541 ccagatccat cgtcagctcg acgggtctgc cggaatcgct gcgcaacagt gcagccggat
     3601 cgtcatacgc ctgtcccgtt cctcgcaccg acaccgtaca ggcttgcagc ggttcagtgg
     3661 cctcgaggct gagttcgatc gcgccggtgt catggaagtc gaccgcggcg atggtcggca
     3721 tatcaggtcc gcatagcagc gcggtgatcc acgccgtctt ctcgttgggc atcagcccca
     3781 atcgtatcca gccgccgaca ccctgcccgg ggtcggtgaa gtcgaagtac cagctctcgt
     3841 tccacaacgg ttcgtcggtc ggatcgtggg caccctcgtc gtcggcgacg ggcgtaagcg
     3901 gcacggctgc aacgggttcg ggcagcacgg ccagcgcatc cgtgtcgagc acatgctgac
     3961 aatgccgccg cagcatcacc atgaacatct cgtcaccacg gctggtctgc gcgacgagca
     4021 tcgacgagat gatcgccatc aacaccccga agaagctctg ccgacgcacc ccgtcacgca
     4081 cctcgtcgag actgagcttg gctccgagcg cctgatggta ggcccgcagc agcgcgtcat
     4141 agtgatcgcg gcgcaggcgg tcgggcagcg cgcagccgag gaagtatgcg acgtcggtca
     4201 tcgccggtcc ccacgtcacg gtctgccagt ccaccaccgt gagcgggcgg tcggcgccag
     4261 cctcgccgaa tagcatgttg tccaggcgat agtcgccgtg caccagcccg ggtacccgat
     4321 cggattcgct tgcgacgtaa gcatcgaagg ttgcgaccag ccgctcgcac acctcgcgat
     4381 gctccggagc gatctgatcg ccgtagcggt caatgaaccc ggcgtacaac tggccgatca
     4441 ctgcctggtt catcggcgac tcccggttga gccagtcggc gttcgccatc gcggtgtcgc
     4501 ccagcagcgg ggcgtgcacc cggcccagtt ccgacagcgc cagcatggcc tgttctactg
     4561 tcgcgccgcg gatctcgtca ccgacgacgg ccgggcctgc gtcacccagc agcaggtgaa
     4621 aggcgcccgt ctcggtgtcg aaggcggcgt ggtggcacgg cgcgatcggt ccgtcgatgc
     4681 ccggtgcaat gtcggtgtag aaccgcacct cacgttcgta cagaccgagc gcgagaccgg
     4741 tctgacgact caccgggtcg gtggcagcga ccttgagcac cacagagttc gggccgccgg
     4801 cgccctcagc gtaggtcagc gcgacgcggt agcactcgct catctggccc gtgccgatgc
     4861 gttcgacggc gaaatcgctg accgattgac ccagcgtggc ggtgagccat tcggccgtca
     4921 gatcggccgg ccgctccacg acgtcagtgg tgcgcactgg tcgtcggggt atgcgcgccg
     4981 ggcacgtcga gtgacgggtt gcggtcgaag aagccgaacg gcttgagcca gaaggacacc
     5041 acgtcgacgg gcatcaccgg ccagtcctcc ggccgtgtga tgtggtgaat gccgaacacg
     5101 taccacatca ccacatcggt gttatcgatg gacctgttgg ccagcgtcca acgacccaaa
     5161 cctgtgtccc gcttcgattg attcacgaat tcccctgcgg gccaccgctc atcgggccgg
     5221 ttcggcgtca cccacaaggt atgaccgatg acactggcgc gctcgacgac gggcgattcg
     5281 gggtcgaaca tcggcggaat cgccccagtc ggaaccaatt tgtaggccgg gtgggtgccc
     5341 agcccgttga ccacgttggt gttgaccacc ttccaggcgc gctgcgtcgc gaagttcaca
     5401 tcctgcttgg cctggctctc ggtgcgcagc gccacgttgc gtacgacgag cgaaagcccg
     5461 tgcggattgt cccggccaat cggttccgga tgcgactccg tcatgtagac ggtgttgtcg
     5521 cttccgtcga tatccatgtc gagccgggcg accagaaagt gttggtgaaa cggcgcatac
     5581 gtgcgttcgt cgacaagcgt gccgttcgga tggggttggc cgggcgccag cggtgtggtc
     5641 accatgatgc cggtggcccg cacctcgcat tcgatgttgc cgtcctggta cagccgccag
     5701 tacacgaggt attcataatt ggcgacggtc acatgaaacg agatcgtcag ccgacgcatc
     5761 cggcgcacct cggcgccgat ttcgtggtca acgtgcttcc acagcacggc gttgtcttcc
     5821 tcatggatac agatcgcgtt ggtgatcgtg tatggctcgc ccttgctgtt gtgcaaaacg
     5881 gcgtccagat aacggatctc gcccaggcaa tcgcagccga gttccagcga cgtcgtcatg
     5941 aagcccaggc cccactcgcc gatgtcgaac gccgtgcggc ggtagtggtc gtcggagggg
     6001 tcgcggtacg ggacaatcat ctccgcgaac gacaaccggt gtgccacaga gcgattccgg
     6061 tcgccgtcgc ggtaacccac ggtgtgcagg gtcatcccct cgcggtggtt gaagccaatg
     6121 cgcagcgacc agttctgcca ctgcagcaga ttgccgtcca acgtgaacga cggcccctcg
     6181 ggttgggtga tgtgcagcgg cttgagcggc tcacggcgtg acgccgagcg gatccgctcc
     6241 gggatgcggc tcggcacgta ttcgcccatc acctcgggtg tgtcgacgcc gccgttgtct
     6301 tcgacccgta gcaactccat cgagttgacg tcgaccacac aatgcagccc gctgacgaga
     6361 ttcgcgtagg ggttggcgcc cggcacggcc ttgacccagg tgtcggacca gccgagccgt
     6421 cggtcgcggt actccggcgg cgccaccgca tccccgtagg tccaggtgtc gaagaacacc
     6481 agatccatat cggtgatgcc gcgtttggcc agggcggcaa tgagatcagg attggcgcgc
     6541 agcaattggt cgcactcgac gaactcgtcg acagtgaagt tcgcctgcac gcccggaacg
     6601 tgctcgaacg actcgacgcg gtcgtcggtc aacgacacca cgctcttgta tgtggcgttg
     6661 tccgaccggt gcaggcaaat cacctcagcg cgtcgcggcg gccgcggtcc gccatcgtcg
     6721 aacgcacgca ggtcggattt agcgggttca gccagctcca ccgacgcgaa ccgccagcca
     6781 tccccgacgc cgtgctcacg ctgcaggatc gacgcgaccg ccctgaactc gtcggcgtcg
     6841 agcggatcca gtgggtgagc cacggggtta cgcaatcaca tccgcgccgc gccgcgcagc
     6901 cgattcggcg acggcgtcag ccggtggcca gggagaatcc ttcccatgcc tgtctgcgcg
     6961 cggcgtgcgg gtcatattcc acgcggggac gccggtcgaa caccatcacc gcgcggtcgg
     7021 cagcggtgta ggcaggccag ccctcgcccg gcgtgccggt tcggctgaag gcatgccagc
     7081 gagcccgcat gtcgtcgctg acgcgcagcg cagtacgcct gtcagctgcg gcagtgagca
     7141 gccggccgaa cttcgtgcgg tagacgtcga acaccgccaa caactcggtg gcatgcgtgg
     7201 cgcccaggcc cgaccagcgc agcgtgcgcg gcgcgtagtc gtaccggtac aggtatgtcg
     7261 gggcgtgtct gctgtgcgcc tcggcgatct gccaagccgc cgatccgaac gcgaagtcgc
     7321 cgccgaactg aatgcaggcc gcaggatcgg gatagcccgg gtacgcggcg gtaatgcgtt
     7381 cgcgctcacc gggttccgcc gcagccagta gccgctcaat catcggctcg gtcatcggca
     7441 gaagcttcag gaagcggccg aacagcctgg cctcctcggc gttggtgcca atgatgagcg
     7501 gcaaacggtg cgccgcgccg acccgcatcg cctcaaccgg gtccagcggc aggcagtccg
     7561 tgccacgggt cgggccggcc gcaaacgcgc cgagcatctc gcgctgaccc tgccgcacca
     7621 gccggtcgaa ggcgttgacc aggtcctccg gccgggctgt cagcacggcg tcggcgccgt
     7681 cctccttgcg ggcaccgagc agcgcagcga acttggtggc gtattcggcg gcaatctcgg
     7741 gagagcggac cattccagcg gccgtgctct ccgaaatcgc ctgagcaaac aggcctttgg
     7801 cggccggaac ggcgagcagc gtggcgacgg cgtgcgcgcc cgcgctctcg ccgaagatcg
     7861 tcacattgtc gggatcacca ccgaacaccg cgatgttgtc acgcacccag cgcagcgcca
     7921 tcaccaggtc ccgcaggaac aggttgtcgt cgaacgtgat gtcctttgtg gacaacgacg
     7981 aaaggtcgag acagcccagc gcgcccagcc ggtaattcac cgagacatac acgcagcctt
     8041 cgcgcgccag cgccgcgccg tcatagatcg gggttgccga actgcccatg aagtagccgc
     8101 cgccgtggat gaagaacatc accggtaacg ggtgcgc
//
LOCUS       DASRJA010000347        19249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3245127,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000347 DASRJA010000000
VERSION     DASRJA010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 19249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19249
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3245127"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            682..2052
                     /locus_tag="VFC01_15405"
     CDS             682..2052
                     /locus_tag="VFC01_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81052.1"
                     /translation="MQRSALLRAELEDAVAGMTPAARAVFEDIQGRDLDADPNLPEDF
                     HALTPIERTSVIGAAKLLKDLAEAETAEEADDQKQPDGVSRLERQLWIGSVLISLAFL
                     IAVGSILVGVYTAPTTPDPADTVVTTPDEVTRFLDAHVPPPQRGAEPPVFIPTGLYIE
                     SIEFSGPYTVEVSGNIWQRYANQLPQDLTKGVLLPEAKEQPTLTEVYRVRRGNDELIG
                     WSFHTTLREQFDYRKYPLGRHQIWLRMWHPDYERNVYLTPDLGAYTSLVPAALPGVDT
                     DLVLEDWDIQQSFFSYRSHRYNTQFGIPGYDADQLHPELYYSIAVKRYLLSPLISRTI
                     VPIVILINLFLVVLMIGRGTKRRENFGVRPAEVIFTGAAFFFAVLIAQISLRDEVKAS
                     GLVYIETLYVVTYFIILGVAINSVLLVGRPNFILFRHHDNMWVRVLYWPVTFLTMLVI
                     TLLTFH"
     gene            2052..2288
                     /locus_tag="VFC01_15410"
     CDS             2052..2288
                     /locus_tag="VFC01_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81053.1"
                     /translation="MERGLASFTSYFDLKSGDQKASVGRDIPALKTIRPHRIPGTSPG
                     DFPSKAWAANVASKMLGGEHGVKALAPSGATVVV"
     gene            2672..3544
                     /locus_tag="VFC01_15415"
     CDS             2672..3544
                     /locus_tag="VFC01_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81054.1"
                     /translation="MTQVEDALTADHRPIVLDDFHHVDPALRAAIAKAIKPLLRKTFI
                     VLVAIPSHSFDPARTVADIGGRMTQFKIPDWTVDELTAIGERGLRQLNVTDPKNELSK
                     LLANYSFGSPHIMQELCYALLSKGLDVHETVKSKIAWIPSNFEQIVEDTATESEPFAF
                     QAILAGRNTKGEGRKDIQLKDGGITDSYGITLLAIRGLIPPIIQRFKAIRQAVSDLTR
                     ESIAKERVVAALRGMAAVAEQNKGGADPLFSYRDEVAYIEDPLLAFFLKYGRWREHAV
                     RDVGDSRGVERPLF"
     gene            complement(4080..4304)
                     /locus_tag="VFC01_15420"
     CDS             complement(4080..4304)
                     /locus_tag="VFC01_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81055.1"
                     /translation="MTTALLPGDLAVLAATSRKIVGNGTMIEARYADIVDRYRRIAPD
                     HSLLSSGAAPLNRRSRIERSGDQTARSSVR"
     gene            complement(4315..4986)
                     /locus_tag="VFC01_15425"
     CDS             complement(4315..4986)
                     /locus_tag="VFC01_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN81056.1"
                     /translation="MTVVDDNYTGHVEPRTAARRTLAGASIVKVSVGPMDNNAYLVTC
                     SQTGETLLIDAANDAEILLELIERYAPKLSLIVTSHQHQDHWLALDRVAKATGVPTAA
                     HQLDSGPLPVKPDRTLAHGDTVKIGELSFDVIHLQGHTPGSVALALDGADEATHLFTG
                     DCLFPGGLGRTTKSADFDSLFNDVTTRVFDVYSDATVVYPGHGDDTTLGAERPHLEEW
                     RERRW"
     gene            5093..7993
                     /gene="uvrA"
                     /locus_tag="VFC01_15430"
     CDS             5093..7993
                     /gene="uvrA"
                     /locus_tag="VFC01_15430"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZN81057.1"
                     /translation="MADRLIIKGAREHNLRSIDLDLPRDALIVFTGLSGSGKSSLAFD
                     TIFAEGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTNRNPRSTVGTI
                     TEVYDYLRLLYARAGTPHCPVCGERIARQTPQQIVDQVLAMEEGLRFQVLAPVVRTRK
                     GEFVDLFDKLNSQGYSRVRVDGVVHSLTDPPTLKKQEKHDIEVVVDRLTVKASSKQRL
                     TDSVETALNLADGIVVLEFVDREDHHPHREQRFSEKLACPNGHPLAVDDLEPRSFSFN
                     SPYGACQECTGLGIKKEVDPDLVVPDPDLTLAEGAIAPWSMGQTAEYFTRMLSGLGDQ
                     LGFDVDTPWKKLPAKARKAILEGCDEQVHVRYRNRYGRTRSYYADFEGVMAFLQRKME
                     QTDSEQMKERYEGFMRDVPCPECEGTRLKPEILAVTLAADRDHKSIAEVAELSIAECA
                     EFLNGLTLGTREQAIAGQVLKEIQSRLGFLLDVGLEYLSLSRAAATLSGGEAQRIRLA
                     TQIGSGLVGVLYVLDEPSIGLHQRDNRRLIETLIRLRDLGNTLIVVEHDLDTIAHADW
                     VVDIGPFAGEHGGRIVHSGPYDELLTNPDSLTGAYLSGKEQIEVPAIRRPADRKRQLT
                     VVGAREHNLREIDVSFPLGVLTSVTGVSGSGKSTLVNDILASVLANKLNGARQVPGRH
                     TRVTGLDKVDKLVRVDQSPIGRTPRSNPATYTGVFDKIRTLFAATTEAKVRGYQPGRF
                     SFNVKGGRCEACSGDGTIKIEMNFLPDVYVPCEVCQGARYNRETLEVHYKGKTVAEVL
                     DMSIEDAAEFFKPITSIHRYLQTLVDVGLGYVRLGQPAPTLSGGEAQRVKLASELQKR
                     STGRTVYILDEPTTGLHFEDIRKLLGVINGLVDKGNTVIVIEHNLDVIKTSDWIVDMG
                     PEGGGGGGTVVADGTPEDVAANPDSYTGHFLAEILDVPRPKPKRRNGRKVSA"
     gene            complement(8065..9228)
                     /locus_tag="VFC01_15435"
     CDS             complement(8065..9228)
                     /locus_tag="VFC01_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HZN81058.1"
                     /translation="MRSFTVEERRARLARRHFLSEPADSIDTVVANLVGLHATDPATP
                     YLSLWARLPGFTVADLDADVYDRRTLVKHLAMRRTLWTVRAVDLPWVQSGASDRVADN
                     ERRRLVADAQKAGIAADGETWLDTACAAVLRHLAEHGAASAKELREALPELAGRYDYA
                     PGKRWGGETPLAPRVLTVLSVRGDIVRGLNDGAWTISRPRWTLMSDWLGVTVDATEPD
                     VSRTELVRRWLRAFGPATVTDVKWWFGNTLTWARHALRDIEAVEVDIDGVAGFVLPDD
                     LEVEPDVEPWCALLPGLDITTMGWYGRDWYVGDHRGQVFDSNGNGGTTAWWNGRIVGG
                     WGQDADGRIEVRLLDDVGRDAKRALTRWADELTAWLDGVRVSPRFPSPLSKLR"
     gene            9347..9829
                     /locus_tag="VFC01_15440"
     CDS             9347..9829
                     /locus_tag="VFC01_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HZN81059.1"
                     /translation="MNRVVVGVVGLAAAATVLVGCSDDKSGPASATAAGGSQVSTGGS
                     TEVKVDGKDLSGLDLNSVTCVKKGGKINVASAAVNGQAGLAVVMSDASPPTVDSLGMT
                     VDGNALAVTNTMGAKVGSAEVKVDGSTYTITGEASGADIKNPMAGMITKPFTVKVTCS
                     "
     gene            9892..11517
                     /locus_tag="VFC01_15445"
     CDS             9892..11517
                     /locus_tag="VFC01_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81060.1"
                     /translation="MTIVSDLERARRLIFAADELAAKELLLSLGDPIEAADRDDLMLE
                     VYALLGEIYLVRTAYDGVEECLRRITDCLGIYQSIQAGTSPESAAQVTMADADVDRMM
                     CRYSRRAQFLRTGLTAAAHGDHEAAEAGLLVLTDDTSTAFPDLAEEHRFLVMYARILC
                     ATALCDDDLHVQSVPLWEQVIDTIENPCGDGEFDDFLLVVGATGYGRFCVETGRLTEA
                     EPWLQRAGARAQKHDWKLATARTQFERATARWSEGDRAECQRLAHEAYPAIAEGYRAH
                     DVSRCWLYFGLLSLSIEALDDADERFGHAERHWREVEKPLHIHRILLQRSWVDVFRKD
                     FAAARARVTEARELLDSWPRHSWLQYARLDDHLGTILRAEALADPVNAETKFEQAAEL
                     KVPAALAVDSVRHAMTDADARMRWATHVSARILAGAFAVAWEWDNTELVSELVEYHSA
                     RGSFSTEPHEQAADWTETATAAVPVDTVDDYALVAAGSPLSGVVALTRLGPLPPLQMD
                     PTTAPILTRYRALAFERYGCDVTADEAAWSTWP"
     gene            11499..12707
                     /locus_tag="VFC01_15450"
     CDS             11499..12707
                     /locus_tag="VFC01_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016890492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZN81061.1"
                     /translation="MVDVAVTLILRFADVGVATYASLRVVGEPARTVTWGLQEPELVA
                     ALDQLSTALPDPRASETRRDAVERAIATGPFATPDTELAIARTLGLSLVAPAAWQLLL
                     ECVASPRATLFVSPSARLARVPWGLLALPGDDKFRLMELVDVLMAAPSNIVNSARTPK
                     CWNDSGDRPPLLVLDPRVPGQRPDSDLGSVLGRPSPETSLAQHFAELMEHRTVLPDVS
                     SAVELFRRTDADRPWLARLLDREPSRLLYVGHATAADGDVGHADRAALHLADENPLTA
                     ADLMSAKLPMPPRIAMIACASGGDYQFDEATGLVAAMILGGAQLVTATLWSLPTTAGY
                     RQFAPATGDHDPMAEAIIGVDRAHQESDAGRAVNSWQRQQMRQWRDGDAGASPVYWAA
                     LATFAVDGAR"
     gene            complement(12712..13194)
                     /locus_tag="VFC01_15455"
     CDS             complement(12712..13194)
                     /locus_tag="VFC01_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81062.1"
                     /translation="MSTEMLLGVAAAEMIAVIFVFVVLADAAATRDAPDPVEPEPSES
                     DERLLTAAEMNAVRAAFDQMLVRKGLVTLHQLDLIRHGTKSRGVVTGMRATGTRREDH
                     REVELDVMVKKPGGGQFPAHETALIPASSLAWVSPGSIIDTYFRSDDESAVAVCVPPR
                     "
     gene            13249..14565
                     /locus_tag="VFC01_15460"
     CDS             13249..14565
                     /locus_tag="VFC01_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81063.1"
                     /translation="MSAGYEVAATAMAVSQPRRAVIDRGWRAIGPGVEVLSGDDGGPL
                     RRTVKRILDPLVLRLRANPQYSAPFVDAATAVAMHDLIVGHGAQLRAAAAWFELLKRE
                     RRRLRITTGNAQELYFPVCFELAVTKGAPAQDDSAEAVLREIHGDRDRTGIEVLNQYV
                     ADPEAVARLTDQLGRSWSDVRAGSEITGPFLAGLTTVLGHAEGHNAATARQRVWTALI
                     ADAAPYNLGANAHTAVVELPWSIVALGLSSIEPQAPPAIVGGHDSDRPLNRSVVDRVR
                     ATLRRAMDRDELPDIPLLCAEEVDRSCAPWGLLAEDKQATLIAGIEIGVSLAPLTESA
                     TSPYDLANQIQARLRKEAYVLHARRYLADGGPIHPRQKHVIDDLAAFARPYLSRLWAR
                     LHGRDVWQEPCDDVDDVRALLEGVARSVSLDHRQRIKAMLELQVAG"
     gene            14562..15647
                     /locus_tag="VFC01_15465"
     CDS             14562..15647
                     /locus_tag="VFC01_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81064.1"
                     /translation="MRLVADSGLWSTGRQAVQPPLVAVLEVIGAVLSWPIDYPSHLAQ
                     ITFTDPARADWLWRVLGESGHSALAAALNERPRDAAVELAGVDVVPGSLEPLRRLALG
                     HWLRRWWPASRRDGIAALDGALLDAEIAVLTARVEDFFAEDTFDSDVAGLLRPHAGAL
                     SAHLQDGDPRVGELVRACADLADDVGIALGEAESVPRRRDDYALAAGADPVGSGSGAI
                     ATGTDSVNWAGVPPRIFDVAENTIAWRVDAADGVAKAAVRVDLSGPGLAAGIAVRLQS
                     GTLGGASMLDADGVATFAVVDEKKQPVTESVAWDHDWRSTAVTVGAGVEELPQTRERI
                     RDFARARLREPAGDAFLAEVLAAESDY"
     gene            complement(15641..15961)
                     /locus_tag="VFC01_15470"
     CDS             complement(15641..15961)
                     /locus_tag="VFC01_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81065.1"
                     /translation="MPDESTPEPEPDAEAKPAESVPVPEAAPAPAEPVDTGYTPGGVP
                     TFESVREKIETRYGTAIGASELAAETPEGRTVEEQYDARQRAAAERLAEIRKSMHPEE
                     GNGQ"
     gene            complement(15980..17377)
                     /locus_tag="VFC01_15475"
     CDS             complement(15980..17377)
                     /locus_tag="VFC01_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZN81066.1"
                     /translation="MHGWLPILVQVIAGTLLVAAIGWRNRRWRLVWLPWTALLGIALA
                     MAAYWYIASQGLGDDINPAPPSLWIWIGLTGVAAGVLIAGWRGVSWWRRSASALALPL
                     CLLCAGLALNLWVGYFPTVQTAWNQLMAGPLPDQTDTGTVVAMQKRHAIPPKGTVVPV
                     SIPNTASGFKHRGELVYLPPAWYATDPPPPLPTVMMIGGEFNTPADWMRAGNAVKTID
                     DFATAHGGNAPVFVFVDSGGAFNNDTECVNGTRGNAADHLTKDVVPFMVSNYRVSPDR
                     ARWGVVGWSMGGTCAVDLTVMHPDMFSAFEDIAGDLTPNSGNKTQTIQRLFGGSAAAY
                     AAFDPTTVITRHGPYAGVAGWFDINGPASTSPPRNVVVDAAARPNDQTAAANSLCATG
                     SVNGIKCAVVSQPGKHDWPFAAHAFAVGLPWLASQLGTPGAPVIPLPASPPAGSPSPP
                     PAPPKSGPHLQAAAK"
     gene            complement(17465..>19249)
                     /gene="lysX"
                     /locus_tag="VFC01_15480"
     CDS             complement(17465..>19249)
                     /gene="lysX"
                     /locus_tag="VFC01_15480"
                     /EC_number="2.3.2.3"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     synthetase/lysine--tRNA ligase LysX"
                     /protein_id="HZN81067.1"
                     /translation="RLWRALLVFFSRWWQLETLYRSNMKYQPVWVPRYACYDDARLIP
                     RVGVASVIAEGFLVLPFSRRNEQPHTGQHISAPENLVATGLLHHDGSAPDLGDLEVDL
                     PAADEGPRLPEQVRVRMAKLKSLQSDGVDAYPVGEAPSHTIAQAVDSEGAGTVTVAGR
                     VLRIRDYGGVLFAQLRDWSGEVQLLLDNSMLDTGPTADFTGAIDLGDLIQVSGTMGYS
                     KKGTRSLVVLSWRLIGKCLRPLPDKWKGLTDQEARVRARYVDLAINTEARDLIRARSA
                     VLHAIRETLVGKGFLEVETPILQQIHGGANARPFLTHINAYDLDLYLRIAPELYLKRL
                     CVGGVERVFELGRAFRNEGVDFSHNPEFTLLEAYQAHADYNVWIDGCRELIQNAAQAA
                     NGAQVFMRPRDDGALEPIDISGAWTVKTVHGAVSEALGEQIAPDTSLTTLRKLCDAAG
                     IPYLTHWDAGAVVLELYERLVEDRTEEPTFYKDFPTSVSPLTRPHRSIPGVAERWDLV
                     AWGVELGTAYSELTDPVEQRRRLQEQSLLAAGGDPEAMELDEDFLQAMEYAMPPTGGL
                     GMGVDRVVMLITGRSIRETLPFPLAKPR"
BASE COUNT         3350 a         6214 c         6354 g         3331 t
ORIGIN      
        1 cgttcggata caccgccgtc ggtgagcaag tcggcatggc ccagcggatg gaatcggtcg
       61 ccccgccggg cgacgccgca tcatcgcacc ccacgacaga cgcaacgaca cctgggacga
      121 ctgggacggc ctagacccgc aaggcgactg gcccgcggag ggctagccgc accgaaggta
      181 tccggcggcg cggtcgggca cgctgacccc gatgccgggc tggctcaggg tgagcagcag
      241 ttcgtgaacg accttgtggc cgtcggcttg acgcgttaga acgggtatcg ggccgtggtc
      301 tcagtgggct acagcgtctg cgacctcggc gctgttaaag gttcttcaag aacgccaagg
      361 ctcgcgccca gaggaggcaa acaaggagca acagtcatga ccttctaccg cgaccccgac
      421 acccacaaac tgttcaccga ggacgagatg caggctcgct tcgagaagct tcgagaacga
      481 cctgctgggc ctgatcggta atgaccgtga cgaggaggcc ctggagatga ccttcgagga
      541 ctccgctgcg cgagagcacc tttctttacg aacgactcac cgccacaacg actttctgtg
      601 aaagcccggg ctagaggagg gctgggacgt aataccccag ccctttgctg tctaatctag
      661 ccgcagagac aggagagagg gatgcaacgc agcgcgttgc tccgggcgga gcttgaggat
      721 gcggtggcgg ggatgacccc cgccgctcgc gctgtcttcg aggacataca ggggcgggat
      781 ttggacgcag atcctaactt gccggaggac tttcatgcgc tcacacccat cgagaggacc
      841 agcgtcatcg gggcggcaaa actcctgaaa gaccttgccg aggcggaaac ggctgaggag
      901 gccgacgatc aaaaacaacc tgacggtgtt tcccgactag agcgacagct ttggattggg
      961 tctgtactca tttcacttgc gttcctgatc gcagtgggca gcatattggt tggcgtgtat
     1021 accgcgccga caacgccaga tcctgccgat acagtcgtga ccacccccga cgaggtgaca
     1081 cgtttcctgg atgcgcatgt tccacccccg cagcgtggtg ccgagccgcc ggtttttatc
     1141 cccacggggt tgtatatcga gtccatcgaa ttcagtggac cctataccgt ggaggtgtct
     1201 gggaatatct ggcagcggta cgcgaaccaa ttgccccaag accttaccaa aggcgtcctc
     1261 ctccccgaag ccaaggaaca gccaacgctc acagaggtgt accgggtgcg gcggggcaac
     1321 gacgaactca tcggctggtc gtttcacacg acgctgcgcg agcaatttga ttataggaag
     1381 tatccgctag gccgccacca gatttggctc cggatgtggc acccggacta tgaacgcaat
     1441 gtttacctta cgccggactt aggagcgtat acctcgcttg tgcctgctgc gttgcccggg
     1501 gtggatacgg acctcgttct ggaagactgg gatattcaac agagcttttt tagctatcgc
     1561 agccaccgct acaatacgca gtttggcatt ccgggctacg acgcggatca actgcatccc
     1621 gagctgtact acagcatcgc tgtcaaacgg taccttttga gccctctgat ttcgcgaacg
     1681 atcgtgccta tcgtgatcct cattaatctg ttcctcgtcg tcttgatgat cggcagaggg
     1741 accaaacgac gagagaattt cggcgtacgc ccggctgagg tcatttttac tggcgcggca
     1801 ttcttctttg ccgtccttat cgcgcaaatt tcgctacgtg acgaagtgaa agcatctggt
     1861 ttagtctata tcgaaacctt gtacgtcgtc acgtatttca tcattctcgg cgtcgccatc
     1921 aactcggtgc tgctggtcgg gcggccgaac ttcatactgt ttcgccacca tgacaatatg
     1981 tgggtcaggg ttttgtattg gcccgtgact ttcttgacga tgctcgtcat cacgttactg
     2041 acgtttcatt aatggagcgt gggcttgcct catttactag ctacttcgac ctgaaaagtg
     2101 gtgaccagaa ggccagtgtg ggccgcgata ttcccgcact gaagacgatt cggcctcatc
     2161 gcattccggg gacaagtccc ggcgactttc cttcaaaggc atgggccgcc aatgtggcca
     2221 gcaagatgct cggaggcgag cacggcgtca aggcactggc acccagcggt gcaacggttg
     2281 tcgtctagac gttcaccgaa gttcccgtcg cgctgcgcca ggtggcgata tcgctgttgg
     2341 atatgttgcc gaccaagtcg cacacgacga gcgaggacgc ctacctgcgc cagaccacca
     2401 ggaccgtcgc ggcatccctg catgaagtcg tgtttatctc ggcgcaaacc cgaccgggtg
     2461 tgtcgttgaa ctggctatca ggtctgcgaa cgtgccatcc gccgctttgt cgtagcgcat
     2521 attccaagca tgcgaaccct gacccgattt gtcgccgcgg tggtgacagc cgctgtgatg
     2581 cccgcgtgtt cggtcatcgc cagctccgta gcgcatgccg atccggtagc cacgacggca
     2641 accgtgttga aggtggtcga cggcgacacc gttgacgcag gtcgaggacg cgctgacagc
     2701 agatcacagg ccaatcgttc ttgacgattt ccaccacgtc gacccggcgc ttcgcgctgc
     2761 catcgcgaag gcgatcaagc cgctgctgcg gaagacgttt atcgtgttgg tcgcgatccc
     2821 gtcccactcg ttcgatccgg caagaacagt tgcagacatc ggcgggagaa tgacgcagtt
     2881 caagatcccc gactggacgg tggacgaact aaccgccatc ggagaaaggg gacttcgcca
     2941 actaaacgta accgatccaa agaacgagtt gtcaaaactt ctggcaaact acagctttgg
     3001 ttcgcctcac atcatgcagg agctgtgcta tgcgctcctc tcaaaggggc tggatgtaca
     3061 tgaaacggtg aaatccaaga ttgcttggat tccaagcaat ttcgaacaga tcgtggagga
     3121 cacggctacc gaatccgagc cgttcgcctt ccaggccatt ctggccgggc gcaacactaa
     3181 aggcgagggc cgtaaggaca ttcaattgaa ggacggcggc atcaccgata gttacggaat
     3241 cacgctcctg gctattaggg gcttgattcc tccgatcatt cagcggttca aggcaattcg
     3301 ccaagccgtc agtgacctta ctcgagagtc gattgcgaaa gaacgggttg ttgctgcgct
     3361 ccggggcatg gcggccgtcg cagagcagaa caagggtggc gcggacccct tgtttagcta
     3421 ccgggatgag gttgcgtaca tcgaagatcc gctgttggcg tttttcctga aatatggccg
     3481 gtggcgggag cacgcggtgc gtgacgttgg cgatagcaga ggcgttgagc gcccgctgtt
     3541 ttaaccccgc aatggaatga tgttgccgag ccgagcggct ggccgactca ttgcgccgag
     3601 cttgcacatc tcgtctgcgt ggtcagcctt cttatagacg tgcgtgtaga tgtgctcagt
     3661 tgtcgtcggc ttcgcgtgcc ccatgaattc aacaacctgg cgaaccggaa tcccggcgtc
     3721 accgcacagg ctggcgaggt gcgtttgccc gccatcagcc cgagaagttg gagctgcccc
     3781 tgtgtccaca atctgcctgc agctacagat agcagccagg gacgccgccg tgtgccgcaa
     3841 ggaagccaga tcggcaacgc tgagcgccaa cctccgtctg gattcgtagg atcgtcgctc
     3901 gtgtccgacc aactcccggc gctggctcca cctgcagagg cggctgaata tctgcgtacc
     3961 acgaccgctg ctctcgcaca ggatcgttac cgggggactg acccgaagtt catcaagcac
     4021 ggcagccggg tgctctaccg ctggtccgat gtgtcggaat ggctgggcca caatactttt
     4081 cagcggaccg atgaacgggc ggtttgatcg ccggagcgtt cgatccttga tcgcctgttc
     4141 agcggggcgg cccctgacga gagcagcgag tggtccgggg cgatccttcg gtagcgatcc
     4201 acgatgtcgg catacctggc ctcaatcatc gtgccgttgc cgacgatctt tcgcgaggtc
     4261 gccgccaaga ccgcgaggtc gccgggcagc agcgcggtgg tcactccgcg tcgctcacca
     4321 ccgtcgttcg cgccactctt cgaggtgggg acgctccgca cccagcgtcg tgtcgtcacc
     4381 gtgcccgggg tagaccactg tggcgtcgga gtagacgtcg aacacccggg tggtgacgtc
     4441 gttgaagagc gagtcgaagt ccgccgattt tgttgtccgc ccaagaccgc ccgggaacag
     4501 gcagtcgccg gtgaacaggt gggttgcttc gtcggcccca tccagtgcca gcgcgacgga
     4561 tcctggtgtg tggccctgca gatggatgac gtcgaaggac agctcgccga tcttgacggt
     4621 gtcgccgtgg gccagcgtgc ggtctggctt gaccggcagc ggtccggagt cgagctgatg
     4681 cgcggctgtc ggcaccccgg tggccttggc gacccggtca agcgccagcc aatggtcctg
     4741 gtgctgatgg ctggtgacga tcagcgacag cttcggggcg taccgctcga tcagttcgag
     4801 caggatctcg gcgtcgttgg cggcgtcgat gagtagcgtt tcgcctgttt gggaacacgt
     4861 gaccaggtag gcgttgttgt ccatcgggcc caccgagacc ttgacgatcg acgcgcccgc
     4921 gagggtgcgc cgggccgcgg tccggggttc gacgtggccg gtgtagttgt cgtcgacgac
     4981 ggtcatggtg ctcacgttat cgaacggctc cacagcgccc gcgaagttgt cggtgggtgg
     5041 acatagcatg ggcatgaact ctgtgctgcg ggacggacag gaagagacac agttggctga
     5101 ccgcctgatc atcaagggtg cgcgtgagca caacctgcga agcatcgacc ttgacttacc
     5161 gcgcgatgcg ctgatcgtgt tcaccggatt gtccggttcg ggtaagtcgt cgctggcgtt
     5221 cgacacgatc ttcgccgagg ggcagcggcg ctacgtcgaa tcgctgtcgg cgtatgcccg
     5281 gcagttcctc ggtcagatgg acaagcccga cgtcgacttc atcgagggcc tgtcgcccgc
     5341 ggtgtccatc gatcagaagt ccaccaaccg caacccgcgg tcgaccgtcg gcaccatcac
     5401 cgaggtgtac gactacctgc gtctgctgta tgcgcgggcc ggcaccccgc actgcccggt
     5461 gtgcggagag cggatcgccc gccagacccc gcagcagatc gtcgaccagg tgctcgccat
     5521 ggaggagggc ctgcggttcc aggtgctggc accggtggtg cgtacacgta agggcgagtt
     5581 cgtcgacctc ttcgacaaat tgaactccca gggctacagc cgggtgcgcg tcgacggggt
     5641 ggtgcactcg ctgaccgacc cgccaacgct gaagaagcag gaaaagcacg acatcgaggt
     5701 cgtcgtcgac cggttgacgg tcaaggccag ctccaagcag cggttgaccg actccgtgga
     5761 gacggcgctg aatcttgccg acggcatcgt ggtgttggag ttcgtcgacc gcgaggacca
     5821 ccacccgcac cgcgagcagc ggttctcgga gaagctggcc tgcccgaacg gccacccgct
     5881 ggccgtcgat gaccttgagc cgcggtcgtt ctcgttcaac tcgccgtacg gcgcctgcca
     5941 ggaatgcacg ggtcttggca tcaagaagga ggtcgacccc gacctcgtgg tccccgaccc
     6001 cgacctgacg ctcgctgagg gcgccattgc gccgtggtcg atggggcaga cggccgagta
     6061 cttcacccgg atgctgtcgg gtctcggcga tcagctcggg ttcgatgtgg acaccccgtg
     6121 gaagaagctt ccggccaagg cgcgcaaggc aattctcgag ggctgtgacg agcaggtgca
     6181 cgtgcggtac cgcaaccgct acggccgaac ccgttcgtac tacgccgatt tcgaaggcgt
     6241 gatggcgttc ctgcagcgca agatggagca gacggactcc gagcagatga aggaacgcta
     6301 cgagggcttc atgcgcgatg tgccgtgtcc ggaatgcgaa ggcaccaggc tgaagccgga
     6361 gatcctggcc gtcacgctgg cggccgaccg ggaccacaag tcgatcgcgg aggtcgctga
     6421 gctgtcgatc gccgaatgcg ccgagttcct caacgggctg accctcggga cgcgcgagca
     6481 ggcgatcgcg gggcaggtgc tcaaggagat tcagtcgcgg ctggggttcc tgctcgacgt
     6541 cgggctcgag tacctgtcgc tgtcgcgagc ggccgccacg ctgtccggcg gtgaggcgca
     6601 acgcattcgg ttggccaccc agatcggctc cggactcgtc ggtgtgctct acgtgctcga
     6661 cgagccatcg atcggtctgc atcagcgcga caaccggaga ctgatcgaaa ccctgatccg
     6721 gctcagggat ttgggcaaca cgctgatcgt cgtcgaacac gaccttgaca ccatcgccca
     6781 cgcggactgg gtggtcgaca tcggcccctt tgcgggcgag cacggcggcc gcatcgtgca
     6841 cagcggcccc tacgacgagc tactgaccaa cccggattcg cttaccgggg cgtatctttc
     6901 gggcaaggag cagatcgagg tccccgcgat ccggcggccg gctgaccgca agcgtcagct
     6961 cacggtggta ggtgcgcgcg aacacaacct gcgtgagatc gacgtgtcgt tcccgctggg
     7021 cgtgctcacg tcggtgacgg gtgtctcggg ttcgggcaag tcgacactgg tcaacgacat
     7081 cctggcgtcg gtgctggcga acaagctcaa cggcgcccgt caggtgccgg gccggcacac
     7141 ccgcgtgacc gggctggaca aagtggacaa gctggttcgg gtcgaccagt cgccgatcgg
     7201 ccgcaccccg cggtcgaatc cggcgaccta taccggcgtc ttcgacaaga tccgcacgct
     7261 gttcgccgca acgaccgagg cgaaggtgcg cggctatcag ccgggccgat tctcgttcaa
     7321 cgtcaagggc ggtcgctgcg aggcatgctc cggcgacggc accatcaaga tcgagatgaa
     7381 cttcctgccc gacgtgtacg tcccatgcga ggtctgccag ggcgcccggt acaaccgcga
     7441 aacgctcgag gtgcactaca agggcaagac cgtcgccgag gtgctcgaca tgtcgatcga
     7501 ggacgcggcg gagttcttca agccgatcac ctcgattcac cggtatttgc agacgctggt
     7561 cgacgtcggg ctgggctacg tgcggctggg gcaaccggcg ccgacacttt ccggcggcga
     7621 ggcgcagcgc gtgaagctgg cgtccgaact gcagaagcgg tcgacgggtc gcacggtgta
     7681 catcctcgac gagccgacca ccgggctgca tttcgaggac attcgcaagt tgctcggtgt
     7741 catcaacggc cttgtggaca agggcaatac ggtcatcgtc atcgagcaca acctggacgt
     7801 gatcaagaca tcggactgga tcgtcgacat gggtccggag ggcgggggcg gcggcggcac
     7861 cgttgtcgcg gacggaacgc cggaggacgt cgccgcgaac ccggacagct acacggggca
     7921 cttcctcgcg gagatactcg acgtgccacg gcccaagccc aagcgtcgca acggccgcaa
     7981 ggtcagcgcc tagccctgtc gccgaaactg ttcccggctt cgcgtgggcc cagccagatc
     8041 gcgatccggc aggatttcgc gatctcaccg cagtttcgaa agcggcgacg ggaagcgggg
     8101 gctgacccgt acaccgtcca gccacgccgt gagctcgtcg gcccacctgg ttaaggcccg
     8161 cttcgcgtcc cggccgacat cgtcgagcag tcgcacctcg atccggccgt ccgcgtcctg
     8221 accccagcca ccgacgatcc ggccgttcca ccatgcggtc gtcccgccgt tgccgttgct
     8281 atcgaacacc tggccgcggt ggtcgccgac ataccaatcc cggccgtacc agcccatcgt
     8341 cgtgatatcc agcccgggca acagtgcaca ccacggctcg acgtccggct caacctccag
     8401 atcgtcgggc aacacgaaac ctgcgacgcc gtcgatgtcg acttcgaccg cctcgatgtc
     8461 acgcagtgca tgtcgcgccc acgtcagcgt gttgccgaac caccatttga cgtcggtgac
     8521 ggtggcaggc ccaaaggcgc gcagccatct gcgcacgagt tcggtgcggg acacgtccgg
     8581 ttcggtggcg tcgaccgtga cacccaacca gtcggacatc agcgtccagc ggggccgcga
     8641 tatcgtccac gcgccgtcgt tgagtccccg gacgatatcg ccgcgcaccg atagcaccgt
     8701 caaaaccctt ggcgccaagg gtgtctcgcc gccccagcgc ttacccggcg catagtcgta
     8761 gcggcccgcc agctcgggca gtgcctcccg taactctttc gcgctcgccg ccccgtgttc
     8821 ggcgaggtgc cgcaacaccg ctgcgcacgc ggtatccagc cacgtctcgc cgtccgccgc
     8881 aatacccgcc ttctgtgcgt ccgccaccag tcgacggcgc tcgttgtcgg ccacccggtc
     8941 gctcgccccc gactgcaccc acggcagatc aaccgcacgc actgtccaca gcgtgcggcg
     9001 catcgcgagg tgcttgacca gcgtgcgtcg gtcatagacg tcggcgtcca gatcggcgac
     9061 ggtgaagccg ggcagccgcg cccacagcga tagatacggc gtggccggat cggtggcgtg
     9121 cagcccgaca agattcgcca ccactgtgtc gatcgaatcc gccggctcag acaggaaatg
     9181 cctccgcgcc agccgcgccc gtcgttcctc gaccgtgaat gaccgcatcg cctgcgaacc
     9241 gtacctgcgg cgaccgacaa agtttgggat cggtagccat ccggccgatc ttgactgtcc
     9301 tcacgacgga cgccatcggg gcgacgtacg acgaagggaa agatcgatga atcgagttgt
     9361 cgtgggtgtg gtcggactgg ccgccgccgc aactgtgctg gtgggctgca gtgacgataa
     9421 gagtgggccc gcttcggcca cggccgcggg tggctcacag gtcagcacgg gcggcagcac
     9481 cgaggtgaag gtcgacggca aggacctctc cggcctcgat ctgaactcgg tgacctgcgt
     9541 caagaagggc ggcaagatca acgtggccag cgctgcggtc aacggccagg cgggcctcgc
     9601 cgtcgtgatg agcgacgcga gcccccccac cgtcgactcg ctgggcatga cggtcgacgg
     9661 caatgccctc gccgtcacca acaccatggg cgcgaaggtc ggctcggccg aggtcaaggt
     9721 cgacggaagc acctacacca tcaccggcga agcttcgggt gccgacatca agaacccgat
     9781 ggcagggatg atcaccaagc cgttcaccgt gaaggtgacg tgcagctgat cggtcccgct
     9841 cccagagcgg cgggcgtgcc tcctgcgccc gccgcttctt atcattcagc catgacaatc
     9901 gtcagcgacc tcgagcgcgc gcgtcggctg atattcgccg ccgacgagct ggcggcgaag
     9961 gaattgctgc tgtctctggg ggacccgatc gaggccgccg atcgggacga tctgatgctc
    10021 gaggtctacg cactactcgg tgagatctac cttgtccgca ccgcctacga cggtgtcgag
    10081 gagtgcctcc gccggatcac cgattgtctg ggcatctacc agtcgatcca ggctggcacc
    10141 agtcccgagt ctgccgcaca ggtgacgatg gccgacgccg acgtcgaccg catgatgtgc
    10201 cggtattcgc gccgcgcaca gttcctccgg acgggactga ccgcggcggc gcacggtgac
    10261 cacgaggccg cggaggccgg gctgctggtg ctgacagacg acacttcgac ggcgttcccc
    10321 gacctcgcgg aggaacacag gttccttgtc atgtatgcgc ggatcctgtg cgcgacggcg
    10381 ctatgcgacg acgacctgca cgtgcagtcg gtcccactat gggagcaggt gatcgacacc
    10441 atcgaaaacc cctgtggcga tggcgagttc gacgactttt tgcttgtcgt cggtgccacc
    10501 ggctacggcc ggttctgcgt cgagaccggg cgattgaccg aagccgaacc gtggcttcag
    10561 cgcgccgggg cgcgcgccca gaagcatgac tggaaattag cgacggccag aacacaattc
    10621 gagcgcgcca ccgcgaggtg gtcggagggc gatcgcgcag agtgccaacg cctggcgcac
    10681 gaggcctatc ctgcgatcgc cgagggctac cgcgcgcatg acgtgtcccg gtgctggctg
    10741 tacttcggcc tgctcagtct gtccatcgag gcgctggacg acgccgacga acgcttcggg
    10801 catgccgaac gccactggcg tgaggtggag aagccgctgc acatccaccg gattctgttg
    10861 cagcgcagct gggtcgacgt cttccgcaag gatttcgccg cagcgcgggc acgcgtcacc
    10921 gaagcccgcg agctgctcga ttcgtggccc cggcacagct ggctgcagta cgccaggctc
    10981 gacgaccatc ttggcaccat cctgcgggcc gaggcgctgg ccgacccggt caacgcggaa
    11041 accaagttcg agcaggccgc tgagctaaag gtccctgcag cgctcgccgt cgactcggtg
    11101 cggcacgcca tgaccgacgc cgacgcgcgg atgcggtggg cgacgcatgt ctccgccaga
    11161 atcctggcgg gcgcgttcgc tgttgcgtgg gagtgggaca acaccgagct cgtcagtgag
    11221 ctcgttgaat atcacagtgc acgaggaagt ttcagcaccg aaccgcacga gcaagccgcc
    11281 gactggaccg agaccgcgac ggccgcggta ccggtcgaca ccgtcgacga ctacgctctc
    11341 gtcgccgcag gctcgccgct gtcgggagtc gtggccctga cccggctcgg gccgctgccg
    11401 ccgctacaga tggaccccac caccgcgccg atcctgaccc ggtatcgtgc gctggcgttc
    11461 gagcgctacg ggtgcgatgt caccgccgac gaggcggcct ggtcgacgtg gccgtaaccc
    11521 tcatcctccg cttcgccgac gtcggcgtcg cgacctatgc cagcctccgg gtggttggcg
    11581 agccggcgcg cacggtcaca tggggattgc aggaaccgga actggttgcg gcgcttgacc
    11641 aactgtcgac agcgctgccg gacccacgcg cctccgagac acggcgtgac gccgttgagc
    11701 gcgccatcgc cacggggccg ttcgcaacgc ccgacacgga actggcgatc gcccggacct
    11761 tgggtctgtc actcgtggcg ccagcggcgt ggcagctgct actcgaatgt gtcgcatctc
    11821 cgcgcgccac gctgttcgtc tcacccagcg cccggctggc gcgggtgccg tggggcttgc
    11881 tcgctctgcc aggtgacgac aagtttcgcc tcatggaact cgtggacgtg ctgatggcgg
    11941 cgccgtcgaa catcgtgaac tccgcgcgca caccgaagtg ctggaacgat agcggcgacc
    12001 gcccaccgct gttggtgctc gacccgcggg tacccggcca gcgccccgac tcggacctcg
    12061 gctcggttct cggtcgaccg tcaccggaaa cctcgctcgc acaacacttt gcagaactca
    12121 tggagcaccg gactgtgctg ccggacgtct ccagcgcggt cgagctgttc cgccgcacgg
    12181 acgccgaccg gccctggctg gcgcgcctgc tcgaccggga accgagccgg ctgctgtacg
    12241 tcggccacgc gaccgcagcc gacggcgacg ttggccacgc cgaccgggcc gcgctgcatc
    12301 tcgccgacga aaacccgctg accgccgcgg acctgatgtc ggcgaaactg ccgatgccac
    12361 ctcgaatcgc catgatcgcc tgcgcatccg gcggtgacta ccaattcgac gaggcgacgg
    12421 gtttggtggc cgcgatgatc ctcggcggag cacagctggt gaccgccaca ttgtggtcgc
    12481 taccgaccac agcggggtac cgccagttcg cgcccgccac aggcgatcac gacccgatgg
    12541 ccgaggcgat catcggcgtc gaccgggcac accaagaatc ggacgcgggg cgtgcggtga
    12601 acagttggca gcgccagcag atgcggcaat ggcgagacgg cgatgccggc gccagccctg
    12661 tctattgggc ggcgctggcc acattcgcgg tggacggtgc gcggtgagcc ctcagcgggg
    12721 cggtacgcat accgccacag ccgactcgtc gtccgaacgg aagtacgtgt cgatgatgct
    12781 gccgggggat acccatgcca gcgatgacgc cggaatcaaa gccgtctcat gggcgggaaa
    12841 ttggccgcct cccggctttt tcaccatcac gtcgagttcg acctcgcgat ggtcctcacg
    12901 acgggttccg gtggcacgca ttccggtgac cactccgcgt gatttggtcc cgtgccggat
    12961 caggtcgagc tggtggaggg tgaccaggcc ttttcggacg agcatctggt cgaatgcggc
    13021 gcgaacggcg ttcatctcgg cggccgtgag caggcgttcg tcggactcac tgggctccgg
    13081 ttcgaccgga tccggcgcat cccgcgtcgc cgcagcatcg gcgaggacga cgaaaacgaa
    13141 aatgacggcg atcatctcgg cggcggcgac gccgagcaac atttcggtgg acatgccatc
    13201 agggtctgcc cttgcgctgg ctaccgaacc caactttatg ctggaccgat gagcgcgggg
    13261 tatgaggtgg cggcgacggc gatggccgtc agtcagccgc gccgtgccgt gatcgaccgc
    13321 ggatggcgtg ccatcgggcc cggcgtcgaa gtgctcagcg gcgacgacgg cggaccgctg
    13381 cgtcgcaccg tcaagcggat cctcgacccc ctggtgctgc ggctgcgggc gaatcctcag
    13441 tactcggcgc cgttcgtcga tgccgccacc gccgtggcga tgcacgacct gatcgtcggg
    13501 cacggcgccc agttacgtgc cgccgcagcg tggttcgagc tgctcaaacg ggaacgacgc
    13561 cggctgcgca tcaccacggg caacgcccag gagctgtact tcccggtctg cttcgagttg
    13621 gcggtgacga aaggtgcacc cgctcaagat gattcggcgg aagcggtgct acgcgaaatt
    13681 cacggtgacc gcgaccgcac tggaatcgaa gtgctcaacc agtacgtggc tgatcccgag
    13741 gcggtcgcgc ggctgaccga tcagctcggc cgcagctgga gcgacgtacg ggccggctcg
    13801 gaaatcaccg gtccgttcct ggcgggcctg accacggtgc ttggccacgc ggaggggcat
    13861 aatgccgcga cggcgcggca gcgggtgtgg acggcattga tcgccgatgc cgctccctac
    13921 aacctcggtg ccaacgcgca caccgccgtg gtcgagctac cgtggtcaat tgtcgcgttg
    13981 ggcctgagtt cgattgagcc gcaagcccca cccgcgatcg tgggcgggca cgacagcgat
    14041 cgtccgctga accggagcgt cgtcgaccgg gtgcgtgcca cgctgcggcg ggcgatggac
    14101 cgagatgaac tgccggacat tccgctgctg tgcgcggagg aagtcgaccg gtcgtgcgcg
    14161 ccgtggggtc tgttggcgga agacaagcag gccacgctta tcgctggcat cgaaatcggc
    14221 gtgtctctgg caccgctaac ggagtccgca acgtcgccgt atgacttggc caaccagata
    14281 caggcccggc tgcgcaagga agcctatgtg ctgcatgcgc gtcggtatct cgccgacggc
    14341 gggcccatcc atccgcggca aaagcatgtg atcgacgatc tcgccgcgtt cgcccgcccc
    14401 tatttgagcc ggttatgggc gcgtctgcat ggacgcgacg tctggcagga accatgcgat
    14461 gacgtcgacg acgtccgcgc gctgctcgag ggggtggcga ggtcggtgag cctcgaccac
    14521 cggcagcgga tcaaggccat gctcgaactg caggtggccg gatgagactc gtcgccgatt
    14581 ccgggttgtg gagcacgggt cgacaagccg tgcagccacc acttgttgcc gtgctggaag
    14641 tcatcggtgc cgtactgtcg tggccgatcg actacccgtc gcacctcgct cagatcacgt
    14701 tcacagatcc ggcccgggcg gattggctgt ggcgtgtcct cggcgagtcc gggcacagcg
    14761 cgctcgccgc ggcactgaac gagcgcccgc gcgacgcggc cgtcgaactc gcgggcgtcg
    14821 acgtagtgcc cggatcgctc gaaccgctgc gccggctcgc gcttgggcat tggctgcgtc
    14881 gctggtggcc ggcaagccgg cgcgacggca tcgcggccct cgacggcgcg cttctcgacg
    14941 ccgagatcgc ggtgctcacg gcgcgcgtcg aggacttctt cgccgaagac acgttcgact
    15001 ccgacgtggc cgggttgctc cgcccccatg cgggtgcgct gagcgcacac ctgcaggacg
    15061 gcgatccgcg agtaggcgag ttggtgcggg cctgcgccga cctcgccgac gatgtcggca
    15121 ttgccctcgg cgaagccgag agcgtgcccc gtcgccgcga cgactacgcg ctggccgcag
    15181 gcgccgatcc ggtcgggagc ggttcgggtg cgatcgccac aggcactgac tcagtcaact
    15241 gggctggcgt cccgccccgg atcttcgacg tggcggagaa caccatcgcg tggcgggtcg
    15301 atgcggccga tggcgttgcg aaagccgctg tgcgcgtgga cttatcgggt cccggactgg
    15361 ccgcagggat cgctgtgcgg ctgcagtccg gcactctcgg tggcgcgagt atgctcgacg
    15421 ccgacggcgt tgcgacattc gcggtggtgg acgaaaagaa gcagcccgtc accgaatcgg
    15481 ttgcgtggga tcacgattgg cggtccaccg cggtgaccgt cggagctggg gtcgaagaat
    15541 tgccgcagac gcgcgagcgg atcagagact tcgcgcgggc gcggctacgc gagcccgccg
    15601 gcgacgcgtt ccttgcggaa gtgctggccg ccgagtcgga ctactgaccg ttgccctctt
    15661 cggggtgcat cgacttgcgg atctcggcga gtcgttcggc ggcggcgcgc tgacgcgcgt
    15721 cgtactgctc ctcgacggtg cgaccctccg gtgtctcagc agccagttct gaagcgccga
    15781 tcgcggtgcc gtagcgcgtt tcgatcttct cgcgtaccga ttcgaacgtc ggcacgccgc
    15841 ccggtgtgta gccggtgtca accggctcgg cgggagcagg cgcggcctcg ggcaccggca
    15901 ccgactcggc gggcttggcc tcagcgtcgg gttccggttc gggagtggat tcgtccggca
    15961 tgccacttac gctaccggcc tacttcgccg ccgcctgcaa atgcggaccg gatttcggcg
    16021 gggcgggcgg aggcgacggc gaccccgcgg ggggcgaggc cggtagcggg atcaccggcg
    16081 cgccgggcgt gcccagctga ctcgcgagcc acggcagccc gaccgcgaac gcgtgcgccg
    16141 cgaacggcca gtcgtgcttg cccggctgcg acacgaccgc acatttgatc ccgttgacgc
    16201 tgcccgtcgc gcacagcgag tttgccgccg cggtttggtc gttggggcgg gctgccgcgt
    16261 ccacgacaac gttgcgcggc ggcgacgtcg atgccgggcc gttgatgtcg aaccagcccg
    16321 ccactccggc ataggggccg tggcgggtga tgacggtagt cggatcgaac gcggcatagg
    16381 cggcagcact gccgccaaac agccgctgga tggtctgggt cttgttgccc gagttcggcg
    16441 tcaggtcacc cgcgatgtcc tcgaacgcac tgaacatatc gggatgcatc accgtcaggt
    16501 cgaccgcgca cgtgcctccc atcgaccacc cgacgacgcc ccacctggcc cggtccggcg
    16561 acacgcggta gttcgaaacc atgaagggca caacatcttt ggtcaggtga tcggcggcat
    16621 ttccgcgcgt gccattgacg cattccgtgt cgttgttgaa cgcgccgcct gagtcgacga
    16681 aaacgaacac cggcgcgttt ccaccgtgcg cggtcgcgaa gtcgtcaatt gtcttgacgg
    16741 cgttacctgc tcgcatccag tcggcgggag tgttgaactc gccgccgatc atcatcaccg
    16801 tcggcagcgg cggcggggga tcggtggcgt accaggccgg cggaaggtag accagctcac
    16861 cgcggtgttt gaaacccgac gcagtgttgg ggatgctgac cggcaccacc gtgcccttgg
    16921 gcgggatcgc atgccgcttc tgcatcgcga ccacggtgcc ggtgtccgtc tgatcgggca
    16981 gcggtcccgc catcagctgg ttccacgcgg tctgcactgt ggggaagtag ccgacccaca
    17041 ggttcagcgc gagtccggcg cacagcagac acagtggcag cgccagtgcc gacgcgctgc
    17101 gccgccacca actgacgccg cgccagcccg cgatgagcac gccggccgcg acgccggtga
    17161 ggccgatcca gatccagagt gacggcggcg ccgggttgat gtcgtcgccc agcccctgcg
    17221 aggcgatata ccagtacgcc gccatcgcga gcgcgatacc gagcagtgcg gtccatggca
    17281 gccacaccag gcgccaacgc cggttgcgcc agccgatcgc ggcgacaagt agggtgccgg
    17341 cgatgacttg caccaatatc ggcagccatc cgtgcatcag cgaaaggtgg tggcgcaacg
    17401 gctcgggtaa cgtcacggca acaatcgtgg acgcgtttcc ttgagatccc ctgtgaacga
    17461 gctgctaacg cggtttggct aacgggaacg gcagggtctc ccggatgctg cgaccggtga
    17521 tcagcatgac gacgcggtcc acgcccatgc cgagcccgcc cgtcggcggc atcgcatact
    17581 ccatcgcctg caggaagtcc tcgtcgagtt ccatcgcttc ggggtcgccg cccgcggcca
    17641 gcagcgactg ctcctgcagc cgcctgcgct gctcgacggg atccgtcagc tcgctgtagg
    17701 cagtgcccaa ctcgacgccc cacgccacca ggtcccagcg ctcggccacc ccggggatgc
    17761 tgcgatgggg ccgggtcagc ggcgataccg aggtcgggaa gtccttgtag aacgtgggct
    17821 cttcggtgcg gtcctcgacg agccgctcgt agagttcgag caccaccgcg ccggcgtccc
    17881 agtgggtcag gtagggaatg cccgctgcgt cgcacagctt gcgcagcgtg gtgaggctgg
    17941 tgtcgggggc gatctgctcg cccagcgcct ccgaaactgc gccgtgcacc gtcttcaccg
    18001 tccaggcacc ggagatgtcg atgggttcca gggcgccgtc gtcgcgcgga cgcatgaaca
    18061 cctgcgcgcc gttggcggcc tgcgcggcgt tttggatcag ctcgcggcag ccgtcgatcc
    18121 acacgttgta gtccgcatgc gcctggtagg cctcgagcag ggtgaactcg gggttgtgac
    18181 tgaagtccac gccctcgttg cggaatgcgc gaccaagctc gaagacgcgc tcaacaccgc
    18241 cgacgcacag ccgcttgagg tacagctcgg gcgcgatgcg caggtacagg tcgaggtcat
    18301 aggcgttgat gtgggtgagg aacggccgcg cgttggcacc gccgtggatc tgctgcagga
    18361 tcggcgtctc gacttccaaa aaccccttgc cgaccagtgt ttcgcggatc gcgtgcagga
    18421 cggcgctgcg tgccctgatg agatcgcggg cctcggtgtt gatcgccagg tcgacgtagc
    18481 gggcgcgtac ccgcgcttcc tggtcggtca atcccttcca cttgtcgggc aatggacgca
    18541 ggcattttcc gatcagccgc caactgagca ccaccagtga gcgggtgccc ttcttgctgt
    18601 aacccatcgt gccggacacc tggatcagat caccgaggtc gatggcgccg gtgaagtcgg
    18661 ctgtcgggcc cgtgtcgagc atcgaattat caagcagcag ttggacttcg ccggaccagt
    18721 cgcgcagctg ggcgaacagt acgccaccgt agtcgcgaat gcgcagcaca cggccggcca
    18781 cggtcaccgt gccggcgccc tcggaatcga cggcctgcgc gatggtgtgg ctcggtgcct
    18841 cgccgacggg ataggcgtcg accccgtctg actgcagcga cttgagcttg gccattcgca
    18901 cgcggacctg ttcgggcagc cgcggtccct cgtctgctgc gggcaggtcg acctcgaggt
    18961 caccgagatc gggtgcgctg ccgtcgtggt gcaacaagcc ggtggccacc agattttcgg
    19021 gcgccgagat gtgttggccg gtgtggggct gttcgttgcg ccgcgaaaac ggcagcacga
    19081 gaaagccttc ggcgatcaca gacgccacac cgacacgggg gatcagccgg gcgtcgtcgt
    19141 agcacgcgta gcgcggcacc catacgggtt ggtacttcat gttcgagcgg tacagcgtct
    19201 cgagctgcca ccaccgggag aagaacacca gcagcgcgcg ccacaaccg
//
LOCUS       DASRJA010000348         3081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3951286,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000348 DASRJA010000000
VERSION     DASRJA010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3951286"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="VFC01_15485"
     CDS             <1..122
                     /locus_tag="VFC01_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN81068.1"
                     /translation="GDRQPEAEALYLSSGYVRLTEPLPAEGEVYPLAFQKALA"
     gene            119..1186
                     /locus_tag="VFC01_15490"
     CDS             119..1186
                     /locus_tag="VFC01_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN81069.1"
                     /translation="MTEHLHVAVALDGYGWHPEAWRRTPDLQPVTSGRYWAGLATTAE
                     RGLLDFVTFDDALTPQRRRRPEIEPRWLAGRPDAVLIASRVAPATSHIGLIPVATVTH
                     TEPFHISKAIATLDFVSHGRAGWQVRVSGTAHEAELFGRRDVTGADLFDEASDAVEVV
                     RRLWDSWEDDAVIRDIATGRFVDRDKLHYIDFAGKYFSVKGPSITPRSPQGQPVVAAL
                     AHALPIYEFASRSTDLVFITPQDDDSLRTILPEVAGLKVYADLYVTFGGDTDPRSDAL
                     VFDGTPTELVELLLSWQRLGVEGVRLRPAVNASDLPVIVNDVVPRLQQAGRFRTAYTD
                     GETLRERLGLPIAENRYARAK"
     gene            1186..2301
                     /locus_tag="VFC01_15495"
     CDS             1186..2301
                     /locus_tag="VFC01_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN81070.1"
                     /translation="MNVPLSILDLAPISEGCNAATALRNTVELAQHAEQWGYKRYWVA
                     EHHFVAVTSSQPAVLIGQIAAATERIRVGAAAVQLGQTTAVAVVESFGMLDAFHPGRI
                     DLGVGRSGQRRREAIKDAESKPKPKPPREWREVDGVIVPPPFDMSALMRNTRLRAKMS
                     VLQQPEAVSPDFGEQVADILAMLDGSYRIGDVDAHATPGEGAGLTPWVFGSSKGQSAQ
                     VAGARGLPFVASYHITPATALDAIDTYRAAFQPSAALSEPYVVVSADVVVADDNATAR
                     QLASSYGHWVYSIRAGDGAIPYPNPDTVKPMTDEQLELVKDRTATQFVGDADEVAAKL
                     ETLQRVTKADELVVTSVTHRHADRLRSHELLAKRWGL"
     gene            2298..>3081
                     /locus_tag="VFC01_15500"
     CDS             2298..>3081
                     /locus_tag="VFC01_15500"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770302.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HZN81071.1"
                     /translation="MTAVREGKDRKPIHLAAHFPGVNNTTVWSDPESGSQIEFDSFVH
                     LAQTAERGLFDFFFLAEGLRLREHRGRIHDLDVVGRPDTFTVLAALAAVTDRLGLTGT
                     INTTFNEPFEVARQFATLDHLSDGRAGWNMVTSSDAFTGENFRRGGFLDHSDRYKRAE
                     EFVTVAREFWDSWAPDAVLADRDSGVYVDPTRVRVVEHRGPQFDVRGVSTLPRAPQGH
                     PVLLHAGDSADGRAFLARYADAAFTMHSSLEAGQAYYADVKHQ"
BASE COUNT          523 a         1087 c          983 g          488 t
ORIGIN      
        1 cgggtgaccg ccagcccgag gccgaggcgc tctacctctc gtccggatac gtccggctga
       61 ccgagccact gcccgccgag ggcgaggtct acccgctcgc ctttcagaag gccctggcat
      121 gaccgagcat ctgcacgttg ccgtcgcact tgacggctac ggctggcacc ccgaagcgtg
      181 gcggcgcacg cctgaccttc agcccgtgac cagcggccga tactgggccg gcctggccac
      241 caccgccgaa cgcggactgc tcgacttcgt gacattcgac gacgcgctca cccctcaacg
      301 ccgtcgccgc cccgagatcg agccgcgttg gctggcaggc cgacccgacg cggtgctaat
      361 cgcgtcccga gtggcgccgg cgaccagcca catcggcctc atccccgtcg ccaccgtcac
      421 gcacacggag ccgttccaca tctcgaaagc cattgccacc cttgacttcg tctcccacgg
      481 ccgcgccggc tggcaggtgc gggtcagcgg caccgcacac gaggcagagc tgttcggccg
      541 ccgcgacgtc accggcgcag atctgttcga cgaggcctcc gacgcggtcg aggtagtccg
      601 ccggctctgg gacagctggg aggacgacgc ggtcatccgc gacatcgcca ccggacgctt
      661 cgtcgaccgc gacaagctgc actacatcga cttcgccggc aagtacttct cggtcaaggg
      721 accctcgatc accccgcgtt cgccgcaggg tcaaccggtc gtcgcggccc tcgcgcacgc
      781 gttgccgatc tacgagttcg cctcgcgcag tacggatctg gtcttcatca caccgcagga
      841 cgacgactcg ctacggacga ttctgcccga ggtggcaggc ctcaaggtct acgccgacct
      901 gtacgtgacc ttcggtggag ataccgaccc gcgctcggat gcattggtat tcgacggcac
      961 cccaaccgaa ctcgttgagc tgctcctaag ctggcagcgg ctcggtgtcg aaggagtgcg
     1021 gctgcggccc gcggtcaacg ccagtgatct gcccgtgatc gtcaacgacg tggtgccgcg
     1081 actgcagcaa gcgggccgct tccgcaccgc ctacaccgac ggcgagaccc tgcgcgaacg
     1141 gctcggactg cccatcgccg agaaccgata tgcgagggcg aagtgatgaa cgttccgctg
     1201 tcgatcctcg acctcgctcc gatcagcgaa ggctgcaacg ccgccaccgc cctgcgcaac
     1261 accgtcgagc tcgcccagca cgccgagcag tggggctaca agcgctactg ggtcgccgag
     1321 caccacttcg tcgcggtgac cagttcgcag cctgcggtgt tgatagggca gatcgccgcg
     1381 gccaccgagc gcatccgcgt cggggccgcc gcggtgcaac tcggccagac cacggcggtc
     1441 gccgtggtgg agagcttcgg catgctcgac gcattccatc ccggccgcat cgacctcggc
     1501 gtcgggcgtt ccggccagcg tcgcagggaa gccatcaagg acgccgaatc gaagccgaag
     1561 cccaagccgc cgcgggaatg gcgtgaggtc gacggcgtca tcgtgccgcc accgttcgac
     1621 atgagcgcgc tgatgcgcaa cacgcggttg cgcgcaaaga tgtccgtcct gcagcagccc
     1681 gaggccgtgt cacccgactt cggcgagcag gtcgccgaca tcctggcgat gctcgacggt
     1741 agttaccgca tcggcgacgt cgacgcgcac gccactccgg gtgaaggggc ggggctcaca
     1801 ccgtgggtct tcggcagcag taagggccag agcgcccagg tggcaggcgc gcgcgggttg
     1861 ccgttcgtgg ccagctatca catcaccccc gccaccgcgt tggacgcgat agacacctac
     1921 cgcgccgcgt ttcaaccgtc ggccgcattg tccgaaccgt atgtggtggt gtctgccgac
     1981 gtggtggtgg ccgacgacaa cgccaccgcg agacaactcg catcgagtta cggccactgg
     2041 gtgtattcga tccgcgccgg tgacggcgcc attccgtatc cgaaccccga taccgtcaag
     2101 ccgatgacgg acgagcagct tgagctggta aaggaccgca cggcaactca attcgtcggt
     2161 gacgcagatg aggtcgcggc gaaactggag acgctgcagc gcgtgacgaa ggccgacgag
     2221 ctggtagtca cgtccgtcac gcaccgacat gcggaccgac tgcgcagcca cgaactgttg
     2281 gccaagaggt ggggcctgtg acagccgtac gagaaggcaa ggaccgcaag ccgattcatc
     2341 tggccgcgca tttccctggc gtcaacaaca cgaccgtgtg gtctgatcct gagtcgggca
     2401 gccagatcga gttcgactcc ttcgtgcacc tggcgcagac tgccgagcgc gggctgttcg
     2461 atttcttctt cctcgccgaa gggctgcggc tgcgtgagca tcgcggccgc attcacgacc
     2521 tcgacgtcgt gggcaggccg gacaccttca ccgtcctggc ggcactggcc gcggtcaccg
     2581 atcgcctcgg cctgacaggc accatcaaca ccacattcaa cgaacctttc gaggtggcac
     2641 ggcaattcgc cacgctcgac cacctgtccg acggccgcgc cggatggaac atggtcacct
     2701 cgtcggacgc gttcaccggc gagaacttcc gtcgcggtgg attcctggat cactccgacc
     2761 gctacaagcg ggccgaggag ttcgtcaccg tggctcgcga gttctgggac agttgggcac
     2821 ccgacgccgt gctcgccgat cgggacagcg gtgtctacgt cgaccccacc cgggttcgtg
     2881 tggtcgagca tcgcggcccg cagttcgacg ttcgcggggt gagtaccctg ccgcgtgcgc
     2941 cgcagggcca tcccgtcctg ttgcacgcgg gtgattccgc cgacggacgc gcgttcctgg
     3001 cccgctatgc cgacgcggcg ttcaccatgc actcctccct ggaggcgggc caggcctact
     3061 acgcggatgt caaacatcag g
//
LOCUS       DASRJA010000349         5612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2199825,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000349 DASRJA010000000
VERSION     DASRJA010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2199825"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFC01_15505"
     CDS             complement(<1..431)
                     /locus_tag="VFC01_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN81072.1"
                     /translation="MAEQFHLSRLQVINWGVFDGYHAIPFSSGGALIAGASGSGKSSL
                     LDAISLGFLPFNRRNFNASGDNTAAGSSAGRRTVDKYVRGAWGQRSDGGTSRVMYLRG
                     DGTAWSAVAVTYASDTGRTVTGLVLKWLTGESRNDSSSRFVL"
     gene            complement(424..1122)
                     /locus_tag="VFC01_15510"
     CDS             complement(424..1122)
                     /locus_tag="VFC01_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HZN81073.1"
                     /translation="MQVTTTDQDADFAAFSELPQVDQTARPPVQRRPRFDGDVSELPD
                     RACWALQHLLTRRYISAEADADVYSWVLEYRKELSVRLSELDLLLRIVDGTDVAFVEQ
                     ARYESERGIKLLRREPLGTYDSILALHLAQMMRANAGQAVVISRDEMHGLISGVLNDT
                     DRDAVTFAARIDGAIARLAGLEILRKTRDDEDSYTISPVITAIMTASVITELQQQFEQ
                     LLTGGAPAEEVEVG"
     gene            complement(1319..1672)
                     /locus_tag="VFC01_15515"
     CDS             complement(1319..1672)
                     /locus_tag="VFC01_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003413177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81074.1"
                     /translation="MEPDGLEARVEALETHVQELDRRVRASEQDAAATRVLAGAADRH
                     VTEFRGELRDFRQATTTSFNALRTEINERFAHVDERFDQVDNGFIEMRGKLDATAAGQ
                     QQIVDILQRLIADQG"
     gene            complement(1750..2862)
                     /locus_tag="VFC01_15520"
     CDS             complement(1750..2862)
                     /locus_tag="VFC01_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HZN81075.1"
                     /translation="MNTARLRVVLRDVEPAVARVIDVPASATLPELHDLLQAAIGWTD
                     SHLYQFVTPQETYGMEIPGGDVWPEDQRDETGARLADLGTEFEYLYDFGDGWTHDVEV
                     LGPGGSTPGCVDGHGTCPPEDCGGPGGYAELLDVLADPAHSEHERMRSWVGNRLRPFD
                     RAATDQWVRRVVGEVPESVRLLLDLAADGIKLTPGGRLPRTVVRSMQQHRPHWYPFDK
                     PVSIEDDLPPLVALHGLLRGVGLLRLRHGVLTPTRAAGDDLAVVRRLRTAFDPHAFDT
                     QITELTIGVLAARGPLPLGQLATQVHQLLGRGWQLDGQPINDEDVRMAIAQQSPIMNG
                     LDLIDQADWRVWVSGPAALSLLPGATMLAEIWTNHE"
     gene            complement(2859..3320)
                     /locus_tag="VFC01_15525"
     CDS             complement(2859..3320)
                     /locus_tag="VFC01_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HZN81076.1"
                     /translation="MTGPEEELAPEPTGGVGAVAFAWLPAGDYEQATRLWPDFAGGDR
                     VAGPDGPLPHPQYCRTMQHILVEYAEAGIPMMVIAPVQVAPFTAWCAERGREPDSAEA
                     RSEYAAHLAATADPCLIAWPPGRNEPCWCGSGRKYKKCCAAPTSVDADPHR"
     gene            3541..3873
                     /locus_tag="VFC01_15530"
     CDS             3541..3873
                     /locus_tag="VFC01_15530"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN81077.1"
                     /translation="MQVKTMGDLGALVRSARTARGMTQADLAARLRMSRDWVVRLEKG
                     HPRLEAQRVLDALRVVGVSLEATVPEAPTNNRRKRGRERAAQKSKSAGTGPAPVDPFE
                     ALFGTRKT"
     gene            3894..5153
                     /locus_tag="VFC01_15535"
     CDS             3894..5153
                     /locus_tag="VFC01_15535"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HZN81078.1"
                     /translation="MYLDGTLIGTATQSSHGSLGFSYDEEYTVRVDPTPLSLSLPILS
                     NRHRDKAVRAYLEGLLPDSEGARQRWASEYHVSPNNPFALLAHVGRDAAGAVQILPPE
                     VDPADARARTGDVEWVSDTDFVDLVEDLATHQSDWDPGRFGGRWSLAGAQPKIALFRD
                     PESGQFGIPRDSTPTTVIVKPAIAGYAQHHVNEALCQRAANEAALLAAESEVLEVGDV
                     QVLISTRYDRLYDGTAWHRVHQEDMCQALSVHPSLKYQSDGGPGVGDIADLLNRLPIE
                     DRDVNAERFFKALAYNVLIGGTDAHAKNYSLILIGSRAQVSPLYDAASAAPYDQRERV
                     RSSMKIGDHWRMLDVTNSDWRKVGRRLGISGDQAVAWVDELRDQLSGAFERATASLPI
                     AVRSEAERIAERIIDHVVGTWKPILGR"
     gene            complement(5235..>5612)
                     /locus_tag="VFC01_15540"
     CDS             complement(5235..>5612)
                     /locus_tag="VFC01_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 family protein"
                     /protein_id="HZN81079.1"
                     /translation="SSIGALGFRIGDLSNPKPAKPGDTEVDLTSFAALTTQVDAPAMS
                     DMLNHAITTGPVSLPDAVAMLDTAYLGHVIVLWSWALKQPNPDDIESATVRFRSLDGR
                     DREIAVPRLTFTEPITTLAGAAT"
BASE COUNT          914 a         1719 c         1982 g          997 t
ORIGIN      
        1 agcacgaagc ggctcgagga gtcgttgcgg gattcgccgg tgagccactt gagcaccagg
       61 cctgtgacgg tgcggccggt gtcgctggcg taggtgaccg cgaccgccga ccaggcggtg
      121 ccgtcgccgc gcaggtacat cacccggctg gtgccgccgt cgctgcgctg gccccaggcg
      181 ccgcgcacgt acttgtcgac ggtgcggcgg cctgcgctgg agccggcggc ggtgttgtcg
      241 ccggaggcgt tgaagttgcg tcggttgaac ggcaggaagc ccagcgaaat ggcgtccagc
      301 agtgaggatt taccgctgcc cgaggcgccc gcgatcagtg cgccgccgct gctgaacggg
      361 atggcgtggt agccgtcgaa gacgccccag ttgatgacct gcagccggga cagatgaaac
      421 tgttcagcca acttcgacct cttctgctgg cgcgccgccg gtgaggagtt gttcgaactg
      481 ctgctgcagc tcggtgatga ccgacgcggt catgatggcg gtgatgaccg ggctgatggt
      541 gtagctgtcc tcgtcgtcgc gggtcttgcg caggatctcc aggccggcga ggcgggcgat
      601 agcgccgtcg atgcgggcgg cgaaggtgac ggcgtcgcgg tcggtgtcgt tgagcacgcc
      661 ggagatcaag ccgtgcatct cgtcgcggct gatgacgacg gcctggcctg cgttcgcccg
      721 catcatctgg gcgaggtgca gggcaaggat cgagtcgtag gtgccgagcg gttcgcggcg
      781 cagaagcttg atgccccttt cggattcgta gcgggcctgc tcgacgaacg cgacgtcggt
      841 gccgtcgacg atgcgcagca gcaggtcgag ttcggagagc cgcaccgaca gttccttgcg
      901 gtattcgagg acccagctgt agacgtcggc gtcggcttcg gcgctgatgt agcggcgggt
      961 gagcaggtgc tgcagggccc agcaggcgcg gtcggggagt tcggagacgt cgccgtcgaa
     1021 gcgtggtcgg cgctggacgg gtgggcgggc ggtctggtcg acctggggga gttcggaaaa
     1081 agcagcgaag tcggcgtcct ggtcggttgt ggtcacctgc attcctcgct gtgtagttgg
     1141 tcggacgccc caacataggt cacggccccg acatgttcgg cgcggcacgg cgccgtgtcg
     1201 ggaggaattc gaggcgtcac cggtttgccg acgtcagcgg aatgtccgcg ctcccaaggc
     1261 atcgcggcca aatggtgccg ctgtgaaaca tgaggcatat ggtgtcaggc cgggtgcctc
     1321 agccttgatc ggcgatgagc ctctggagga tgtcgacgat ttgttgttgg cctgcggctg
     1381 ttgcgtcgag tttgccgcgc atctcgataa acccgttgtc cacctggtcg aaccgctcgt
     1441 caacatgggc gaagcgctcg ttgatctcgg tgcgcagggc attgaagcta gtcgtggtgg
     1501 cctggcgaaa gtcgcggagc tcgccgcgga actcagtgac gtggcgatca gcggcgccgg
     1561 cgaggacgcg ggtcgcggca gcatcctgtt cgcttgcccg tacccgccgg tcaagctctt
     1621 ggacatgcgt ctccagcgcc tccacgcgag cttcaaggcc gtctggctcc acggtcaaga
     1681 gcctaccgtg cacgacgaga gctccagacg ctcatttgtg gtgacggcat gctggccgca
     1741 gctgttggat cactcatggt tggtccagat ctccgccagc atggtggcgc cgggaagcaa
     1801 ggacagggcc gccgggccgc tgacccatac gcgccagtcg gcttggtcga tcaggtcgag
     1861 tccgttcatg atcggggact gctgggcgat tgccatccgc acgtcctcgt cgttgatggg
     1921 ttgcccgtcg agctgccagc cgcgtccgag tagctgatgc acctgcgtcg cgagctgtcc
     1981 aagtgggagc ggaccgcgcg cggcgagcac cccgattgtc agttcggtga tctgggtgtc
     2041 aaaggcgtga gggtcgaacg ccgtcctgag tcggcgcacc acggcgagat catcgccggc
     2101 cgcgcgggta ggggtgagca cgccatggcg tagccgtagc agcccgacgc cgcgcagcag
     2161 cccgtgcagc gccactagtg ggggcaggtc gtcctcgatg gagacgggct tgtcgaacgg
     2221 ataccagtgt gggcggtgct gttgcatgct gcgcacgacc gtcctcggga gccgcccgcc
     2281 cggtgtgagt ttgatcccgt cggcagccaa atcgagtagc agccgcacac tttcgggcac
     2341 ctcaccgacg acccggcgca cccactggtc ggttgcggcg cggtcgaacg gccgcaaccg
     2401 gttgccgacc cagctgcgca tgcgctcgtg ctcggagtgc gccgggtcgg cgagcacgtc
     2461 gagcagttcg gcgtacccgc cgggtccgcc gcagtcttcg ggcgggcagg tgccgtgtcc
     2521 gtcgacgcag ccgggcgtcg agccgccggg cccgagcacc tcgacgtcgt gggtccagcc
     2581 gtcgccgaag tcgtagaggt actcgaattc ggtgccgaga tcggccagcc gggcgccggt
     2641 ttcgtcgcgc tggtcctcgg gccacacgtc cccgccggga atctccatgc cataggtttc
     2701 ctgcggtgtg acgaactggt acagatgcga gtcggtccac ccgatcgcgg cctgcagcag
     2761 atcatgcagc tccggcaggg tcgccgacgc gggcacatcg atcacccggg cgaccgccgg
     2821 ttcgacgtcg cgcaacacca cccgcagacg cgcggtgttc accggtgtgg gtccgcgtcg
     2881 acggatgtcg gtgcggcgca acacttcttg tatttgcgtc ccgatccgca ccagcacggc
     2941 tcgttgcggc ccggcggcca ggcgatcaga cacgggtcgg cggtcgctgc caggtgggcg
     3001 gcgtattcgg accgggcctc ggctgaatcg ggctcgcgcc ctcgttcggc gcaccaggcg
     3061 gtgaacggcg ccacctgcac cggggcgatc accatcatgg ggatgccggc ttcggcgtac
     3121 tcgacaagta tgtgctgcat cgttcggcag tattgcgggt gtggcagcgg cccgtccggg
     3181 ccggccaccc gatcgcctcc cgcaaaatca ggccacaatc tcgtcgcctg ctcgtagtcc
     3241 ccggccggca gccacgcgaa cgccaccgcg ccaaccccac cggtgggctc cggcgcgagc
     3301 tcttcctctg gacctgtcat cggttcgcat cctctcgcac cgctgcgacg cgcaccagcg
     3361 gagggtcgac aagtcggcgc tgcgtcgtca agggatttcg cggccactgc tgcgggacct
     3421 gtctgggcgg caatcacggt atgtctggag tgcggaaagt agcagaacga cgactttcac
     3481 aaaagatgtc gaacggctac aatcaacaat gttgccgaac gactacaagg agtgctggag
     3541 atgcaagtca agaccatggg cgatcttggt gcactcgtcc gttcggctcg caccgctcgc
     3601 ggaatgacac aggccgacct tgccgcgcga ctgcggatga gccgagactg ggtagtccgc
     3661 ctggagaagg ggcatccgcg gctcgaagcg caacgggtgc tcgatgccct tcgcgtcgtc
     3721 ggtgtgagcc tggaggcaac cgtacctgag gcgccgacga acaatcgtcg taaacgcggt
     3781 cgggagcgcg ctgcccagaa gtcaaagtcg gcaggaacgg gtcctgctcc cgtcgatccg
     3841 ttcgaggcgc tgttcggcac gaggaagacg tgatggccgc ccggacactg cgggtgtacc
     3901 tcgacgggac tctgatcggc acggcgacgc aatccagtca tggttctttg ggtttcagct
     3961 acgacgagga atacaccgtg agggttgacc cgacaccgtt gtcgctgtcg ctacctatct
     4021 tgtcaaaccg gcacagggac aaggctgttc gggcgtacct ggaaggtctg cttccggaca
     4081 gtgaaggagc gcgacagcgg tgggcaagcg agtaccatgt gtcgccgaac aatcccttcg
     4141 ctctgcttgc tcacgtcggt cgcgatgcag ccggcgcggt gcagatcctt cctcccgagg
     4201 tcgacccggc cgacgcccgg gctcgaaccg gtgacgttga atgggtcagc gacaccgatt
     4261 tcgtcgacct agtagaggac ctcgccactc atcaatccga ctgggatcca ggaagattcg
     4321 gggggcggtg gagcctcgct ggcgcgcagc cgaagatcgc gctgttccgg gaccccgagt
     4381 ccgggcagtt tgggatcccg cgtgattcga caccgaccac tgtgatcgtc aagcccgcaa
     4441 tcgccgggta cgcacagcac cacgtcaatg aggcgctgtg ccaacgtgct gcaaatgaag
     4501 ccgcgctgct tgccgctgaa tccgaggtgc tggaggtcgg tgacgttcag gtattgatct
     4561 cgacgcgcta tgaccgcctt tatgacggga ccgcgtggca tcgagtccac caggaggata
     4621 tgtgccaagc gctatcggtg caccccagcc tgaaatacca atctgacggg gggcctgggg
     4681 tgggtgatat cgccgacctg ctcaaccggc tcccgatcga ggaccgcgac gtgaacgccg
     4741 aacgcttctt caaagcgctg gcctacaacg tcctcatcgg cggaaccgac gcccacgcga
     4801 agaactactc tctcatcctc ataggttcac gagcccaggt gtcgccgttg tatgacgccg
     4861 cctcggcggc gccgtacgac cagcgcgagc gcgtccgttc ctccatgaag atcggtgatc
     4921 actggagaat gctcgatgtc accaattccg attggcgcaa ggttggacgc cgcctcggga
     4981 tatccggcga ccaggccgtt gcctgggtcg acgaactccg cgaccaactt tccggtgctt
     5041 tcgagcgtgc caccgcgtct ctgccgatag ccgtgcgatc cgaggcagaa cgcatagcgg
     5101 agcggatcat tgatcatgtt gtcggtacgt ggaagcccat tctggggcgc tgagcaccgg
     5161 agccggcggg gcgtggggcg acgccgagcc gcagtttgtc gtggcagtgc tcttcgaagc
     5221 gcggcttgcg ctggttacgt agcggctccg gcgagtgtcg tgatgggttc ggtgaatgtc
     5281 aaacggggga cggcgatctc gcggtcgcgg ccatccaggg aacggaagcg gacggtggcc
     5341 gattcgatgt cgtcggggtt gggctgtttg agggcccagg accacagcac gatgacgtgg
     5401 ccgaggtagg cggtgtcgag catggcgaca gcgtcgggca ggctcacggg cccggtggtg
     5461 atggcgtggt tgagcatgtc ggacatggcg ggcgcgtcga cctgggtggt gagggcggcg
     5521 aagctggtga ggtcgacctc ggtgtcgccg ggtttggcgg gtttggggtt ggacaggtcg
     5581 ccgatccgga agccaagggc gccaatagaa ct
//
LOCUS       DASRJA010000350         5367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3115012,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000350 DASRJA010000000
VERSION     DASRJA010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3115012"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2840
                     /locus_tag="VFC01_15545"
     CDS             <1..2840
                     /locus_tag="VFC01_15545"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZN81080.1"
                     /translation="NVSHEFRTPLTLLLGPLDDAIRDADQPLQRDRLDLARRNADRLL
                     RLVNSLLEFSRIEAGRVTASPVTVDVGALTAQIASSFTELCQRAGIDLVLNCNPVIAD
                     VDVGMWETIVLNLVSNAVKFTLRGSITVAVEADSPESCRVQVSDTGTGIPAADLDRLF
                     ERFYRASNMRGRSVEGSGIGLSLVRSLVELHNGTIGIDSQVDVGTVVTITLPIGRPAS
                     STAVPLNELSIAAWSGDNAYVTEATQWIDKESVESAATLAPDQEPRPLILIADDNSDM
                     RAHLQRLLSERWDTMSFADGQAAIDGIRHHQPDVVITDVMMPSLDGFGLVAALRTDPT
                     LASTPVIMLSARAGVESAGDGFASGADDYLTKPFSSQDLLNRVEARLGAVARQQASRE
                     DDEIRARHDAALADVTAALASADSIHGTMSALLAAPIGSLGVVAMAMGLLDHVNQQIT
                     VHYATPDGEVRHHDAVDREAPAAIVEVARAGRPMVIEDMKDPSALVDDLNRGTSEVGA
                     AAIFPLRDISATVIGAVALLWPTPRTFQPDEIDLIGDVVGVAGTAMARIRAAEHEHRI
                     AADFQDHLLDLDRSSGAVVVSAIYQPASDAMRVGGDWYLVSPVEDSDLVAVCVGDVVG
                     HGLQAAVVMGRLRAATAVTALTAPDPGFVLNAIDRYASTVAGATSATLAFAVVDSANS
                     TIRYMCAGHPYPLLVTSDGVVRYLEDGRLPPLTARIGGSDAEPGQAAFPTGSLLILYT
                     DGLIERHDESLSEGFDRLAQAASACVDQPVDVVCSTLLNQLTPAAGHTDDVVILAVRP
                     TGTTDTSFVATVPADFAQMAAVRHRLRNWLAGLDVGADLEHNIILSVGETLANAIEHG
                     NGLDSETHASVEIFARPAVIDATISDVGQWMADPEALSDESERGRGLTLINGLADRVV
                     ANRTPYGTSVHLQFRRGTGRPGRRSSHGTRT"
     gene            2837..3175
                     /locus_tag="VFC01_15550"
     CDS             2837..3175
                     /locus_tag="VFC01_15550"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN81081.1"
                     /translation="MIRPTFELNIGTVDTKGGVAVRATVTGEIDVANASEFAQSLDAV
                     AGPRPLILDLSPLHYLDSAGFAVVYRLLERHAVIVVLASDSPIHKAATLLGLTCHETI
                     GAAISVGWHE"
     gene            3537..4229
                     /locus_tag="VFC01_15555"
     CDS             3537..4229
                     /locus_tag="VFC01_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN81082.1"
                     /translation="MEQTLSRREASDAVSDQGWRFLLGTFRTSVPVGSLARAAEVAAH
                     AVAACGDDADGHLRIDVRPDRVVLTVQSLDRAALTARDVDIAWRISVAMGEVGLRTEP
                     EIGTAAPRSVQILEIAIDALDIAAIRPFWKAVLGYADEAGVTGPEDPVVDPVGQGPAI
                     WFQQMDQPRPQRNRIHFDISVPHDEAARRIDAALAAGGRLVSAARAPSFWVLADIEGN
                     EACVTTWQGRDG"
     gene            complement(4236..5137)
                     /locus_tag="VFC01_15560"
                     /pseudo
     CDS             complement(4236..5137)
                     /locus_tag="VFC01_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018296659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          899 a         1781 c         1713 g          974 t
ORIGIN      
        1 ctaacgttag tcacgagttt cgcacgccct tgacgctgtt gcttggccca ctcgatgacg
       61 ccatccgtga cgcggatcag ccgttgcagc gcgatcggct ggacttggcg cggcgcaacg
      121 cggaccggtt gctacgtttg gtcaattcgc tactggagtt ctcccgtatt gaagctggtc
      181 gggtcacggc ctcgcccgtg accgttgacg tcggtgcgct caccgcgcag atcgcgtcgt
      241 ccttcaccga actgtgccaa cgtgccggca tcgacctcgt gcttaactgc aaccccgtca
      301 tcgccgacgt tgacgtgggg atgtgggaga cgatcgtcct aaatctcgtg tccaacgcgg
      361 tgaagttcac ccttagaggc tcgatcacgg tagcggtgga agccgactca cccgagtcat
      421 gccgagtaca ggtgagcgac accggaacgg gcattccagc ggccgaccta gaccgattgt
      481 tcgagcggtt ctatcgggca agcaacatgc gcggccgcag cgtcgaggga agcgggattg
      541 ggctctcctt ggtgcgcagc ctagtcgagc tacacaacgg aactatcggt atcgacagcc
      601 aagttgatgt cggaacggtc gtgactatta cccttccgat cggccgtccg gcgtcgtcga
      661 ccgcggtgcc actgaatgag ctctcgatag ctgcatggtc cggcgacaat gcgtacgtca
      721 ctgaagccac acagtggata gataaggaat ccgtggagtc ggccgcaacc ctcgccccag
      781 atcaagagcc tcgtccgttg atcttaatcg ccgatgacaa ctcggacatg cgcgcgcacc
      841 ttcagcgtct cctatccgag cgctgggaca ccatgagctt cgccgatggt caggcggcaa
      901 tcgatggaat tcgccaccac cagccggatg ttgtcattac cgacgtgatg atgccctcgt
      961 tggacggctt cggtctggtt gccgcgttgc ggaccgaccc cacgctcgcg tcgacgccag
     1021 tgatcatgct ctcggcccgc gcgggtgttg agtcggccgg cgatggcttc gctagtggcg
     1081 ccgacgacta tcttacgaag cccttttcat cccaagacct gctcaaccgg gttgaggctc
     1141 gccttggcgc ggtcgcacgg caacaagcaa gccgagagga cgacgagatc cgggcgcgcc
     1201 acgacgctgc cttggccgat gtcaccgcgg cgttggcgtc ggcggattcg attcacggca
     1261 caatgtccgc actcctggcc gctccgattg ggtcgcttgg tgtcgtcgca atggcaatgg
     1321 gtctgttgga ccacgtcaat cagcagatca cagtccatta cgcaacgcct gacggtgagg
     1381 tgcgccatca cgacgccgtt gatcgggaag cgcccgcagc gatcgtggag gtagcccgcg
     1441 ccggacgacc gatggtcatc gaggatatga aagacccaag tgccctcgtc gacgatctga
     1501 accgtggcac ctcagaggtc ggagcagcgg cgatattccc gctgcgcgac atcagcgcga
     1561 ccgtcatcgg agcggtagca ctgttgtggc caaccccgcg aacgtttcaa ccggatgaga
     1621 tcgacctgat cggcgacgtt gttggagttg ccggaactgc gatggcccgc atccgcgcgg
     1681 ccgagcacga gcatcgcatc gcagccgact tccaagacca tctacttgat ctcgaccgga
     1741 gctccggggc tgttgtagtg tcggcgatct accagcccgc atcggatgcg atgcgcgtgg
     1801 gcggcgattg gtaccttgtc tcccccgtgg aggacagcga cctcgttgcg gtctgcgtgg
     1861 gcgacgtagt cggtcacggt ctgcaagcgg ccgtggtgat gggcaggctt cgagcggcca
     1921 ctgctgtcac ggccttgact gcccctgacc caggattcgt gctcaatgcc atcgaccgct
     1981 acgcgtccac tgtggcgggt gccacctccg ctacgctggc cttcgccgtg gttgactcgg
     2041 ccaatagcac catccgttac atgtgcgcgg gccaccctta cccgctgctg gtcacctccg
     2101 atggcgtggt ccgatacctc gaagacggtc gcctgccccc gctgacagca cggatcggcg
     2161 gcagtgacgc cgaaccagga caagcagcgt tccccaccgg cagcctgctc atcctgtaca
     2221 ccgatggact catcgagcgt cacgatgagt ccctgagcga aggcttcgat cggttggcgc
     2281 aagcggcatc ggcgtgcgtc gaccagccag ttgatgtggt ctgctccacg ctgttgaacc
     2341 agctgacacc ggccgccggc cacacggacg acgtggtgat cctcgcggtc cgtcccaccg
     2401 ggacaaccga caccagcttt gtggccaccg tgcccgccga cttcgcgcag atggcggcgg
     2461 tgcgtcaccg cctacggaat tggcttgctg gcctagacgt cggtgcggat cttgagcaca
     2521 acatcatcct tagcgtcggt gagacgctgg ccaatgccat tgagcatggc aacggactgg
     2581 actcagagac acatgcctca gtcgagatct tcgcgcgccc cgcagtgatt gacgcaacca
     2641 taagcgacgt tggccagtgg atggccgacc ctgaggcctt gagcgacgaa tcggaacgcg
     2701 gacgagggct tacactcatc aatggcctcg ccgaccgagt ggttgccaat cgcacaccat
     2761 acggcacgag cgtccatctt cagttccgtc gcgggacggg ccgtcccggg cggcggtcca
     2821 gtcatggaac gcgcacatga ttcgccccac gttcgagctc aacataggca ccgtagacac
     2881 gaaaggcgga gtcgcggtcc gagcaacggt cactggcgag atcgacgtgg ccaacgcatc
     2941 ggagttcgca cagtcgctcg acgccgtcgc tggaccgcgc cctctcattc tcgacctcag
     3001 ccctctgcat tacctggata gcgctgggtt cgccgtcgta tatcgactcc tcgagcggca
     3061 cgccgtcatc gtcgttcttg catcggacag cccgatccac aaagccgcca cgctgcttgg
     3121 tctgacctgc cacgaaacca tcggtgccgc tatcagcgtc gggtggcacg agtgaccgtg
     3181 tacctgccga aaatgtcatc cgagcagctc aggattctcc cgcgccaggc gcaacaccag
     3241 ggcgcgcggg agtctttgcg tccacaatgg tcaatccggc cgctcgacct gtgcgttcaa
     3301 gcgaacgagc catctgggtc gtgcgccata gcgtccacag tcggtgacta cccgacgggc
     3361 aggtcagcag ctatcgccag tcgaccgcct cgggcgcagt tgtacctggt gcccggccgg
     3421 gggcacgcca aacatgggaa acggtgccgg gcgcgtgcgc gccgggcacc gtggccgtgt
     3481 ttcagaattc agccggcgcg cgggaacccg tcgtgggcgc tgcgaggatg tacggcatgg
     3541 agcagacgct gagtcggcgg gaggcgtcgg acgcggtcag cgaccagggg tggcggtttc
     3601 tgcttggtac gtttcgcacc tcggtcccgg tcggttcgtt ggcccgggct gccgaggttg
     3661 cggcgcacgc ggtggcggcg tgtggtgacg acgccgacgg gcatctacgc attgacgtcc
     3721 ggcctgaccg ggtcgttctc accgtgcagt cgctggaccg ggcggcgctg accgctcgcg
     3781 acgtcgacat cgcctggcgg atctccgtcg ccatgggcga ggtcggactg cggacggagc
     3841 ccgagatcgg tacggcagcg ccgcggtctg tccagatctt ggagatcgcg atcgacgcgc
     3901 tggatatcgc ggcgatccgg ccgttctgga aggcggtgct gggctacgcc gacgaggctg
     3961 gcgtcaccgg ccccgaggat cctgtcgtcg acccggtcgg gcagggcccg gcaatctggt
     4021 tccagcagat ggatcagccg cgtccgcaac gcaatcgcat ccatttcgac atctccgtcc
     4081 cgcacgacga ggcagcccgg cggatcgatg cggcgctggc cgctggcggc cggctcgtgt
     4141 ccgcggcccg tgccccttcc ttctgggtgc tcgccgacat cgaaggcaat gaggcgtgcg
     4201 tgacgacctg gcagggccga gacggctgac cgtcttcacg agttccgttc caggcagcgg
     4261 agccagatgt tgatcgcggc gacgcggacg gtggcttcgt agcgcacggc gagtttgtcg
     4321 aagcgggtgg ccacgccgcg gtgctgcttg agcaggttga tgccgcactc cacggcgtgg
     4381 cgctgcttgt atgcctgctt atcaaagctg ggcggccgac cacccttgga tccgagcttt
     4441 cgccggttcg cggcctggtc gtccttgacc ggaatgacgc atctgatccg gcggcggcgt
     4501 aggtaggccc ggttgctctt cgagctgtac gccttgtcgc ccagcaccag gtccggccgg
     4561 gtccggggcc ggccggcatc caggcggggc accctaatgc gttccaggac ggcagtgaac
     4621 tgcgggctgt ctccgtgctg cccggcggtg atcaggatcg acaacggctt gcgggcctgc
     4681 tcgcacccca gatgcagcct cgtggtccac ccacctcgcg agcgtcccaa cgcgtggtca
     4741 gctggctcga ccccggcaaa cccaccgggt ggttccttct gcagatcccc cttttccgcg
     4801 ccccggccgc gtgctggtgc gcccgggcga tggtggagtc cacgctgaca tcccaggtga
     4861 tcagcccggc ggcatccgcg tgcgcctgca acccggtcag aatccgcgcc caggtcccat
     4921 cgcgctgcca gcggcggaac agaccatata cggtctgcca aggcccgtac cgcgccggca
     4981 cgtcccgcca cggcgagccc acccgcaccc gcccccgaat cccgtcgatc aactgccgtc
     5041 tgctgcactt cggcgggcgg cccggcttct tcccattggg cagcaacggc tccagcaccg
     5101 cccactgcgc gtcggtcagg tccgcccgcg ccgccaccgg tagggtgtcc acgaggtctc
     5161 cggggtgttg gtcttcttgg tcgaagaacc atctaccgga gacctcgccg tttatcgatc
     5221 accgacacgc ccatgtggca agtcaccaca gatcacttat gaaacacggc ctagcccggt
     5281 cccccagctg cagtgcccca gcggcgacgc cgcaggcgat agggtcgtct cccggcttag
     5341 caccgccgac ctattattcg caagatt
//
LOCUS       DASRJA010000351         5340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_575591,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000351 DASRJA010000000
VERSION     DASRJA010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575591"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFC01_15565"
     CDS             complement(<1..731)
                     /locus_tag="VFC01_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HZN81083.1"
                     /translation="MTGLPTHVDIRDVSLRDGLQIEEPIPLSAKLELLTAVAATGVRE
                     MEATAFVSPSKVPALADAAELAAKLHNFGGIEFSALVASPNGAKRAIAAGIGSIEYVV
                     SAADGHSRSNVGRSTAESTALIPEIVAIAHDSGVSVEVIIATAWDCPFDGPTPPQRVL
                     EVTTAATAADVDRLAIADTIGTTTPRRVSDLIARIRPLIGDIPLGAHFHNTRGAGLAS
                     AYAAVCAGITRLDASVGGLGGCPFAP"
     gene            complement(728..1933)
                     /locus_tag="VFC01_15570"
     CDS             complement(728..1933)
                     /locus_tag="VFC01_15570"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN81084.1"
                     /translation="MTVTGPLDGIRVIEVGTLISGPFGGRLLGDMGAEVIKIEPPGAP
                     DPLRTWGQAELDGHHFFWTVHARNKKAVTLNLRVEAGRELFLDLVEKSDIIVENFRPG
                     TLEKWNLGYDVLRERNRGIILVRVSGYGQTGPEAHKAGYASVAEAASGLRHMNGFPGG
                     PPPRLALSLGDSLAGMFAAQGALAALYRRTVTGEGQVVDAALTESCLAVQESTIPDYD
                     VGGVVRGPSGTRLERIAPSNIYPTADGSWVVIAANQDTVFRRLCAAMGQPDLATDDRF
                     ANHVARGRNQDELDKIIGAWAAERHPADIIATLSDAGVISGPINTVAEVVRDPQLLAR
                     GMIADHYDERIGRNVKGPGVVPVLSETPGTIRNAGPARPGQHNDEIYHGLLGKSVEEL
                     AALREEGVL"
     gene            complement(1930..3120)
                     /locus_tag="VFC01_15575"
     CDS             complement(1930..3120)
                     /locus_tag="VFC01_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN81085.1"
                     /translation="MTATESTVKYEYDRIPYLIAYENVSGVRDVYGGVSELIVLESYL
                     LKPKTKTSDTVLVFMHPIGGGAYLPMINALARAGHHVIYCNSRFRGTDSALLMEKVVE
                     DLGECIKDAKNRLGYEKVVLAGWSGGGSLSVFYQQQAQHPTITASPSGDGPDLTKLGL
                     IPADGIMMLAAHISRHGTLTEWLDASIVDETDPSKRDPDLDLYDPENPNQPPYTAEFL
                     DRYRAAQIARNRRITKWVKEKLATLAADGRPDDEFAFVVHGTMADPRWLDPAVDPNDR
                     TPGTTYLGDPQVVNMGPFGLARFCTLRSWLSQWSYDDANGDAVKAGPEIKVPTLVIGN
                     LADNACTPSHTRRLFEAIGHPDKEMYEIPGANHYYSGPDQRDTLRQAVGICTDWLHRH
                     GFSL"
     gene            complement(3120..4211)
                     /locus_tag="VFC01_15580"
     CDS             complement(3120..4211)
                     /locus_tag="VFC01_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HZN81086.1"
                     /translation="MESFVHLRKGRTPKRLHADLDGLKDDELGRGGFTGRTANMYRRH
                     DPTAYRCSGPLRPIDVLASELKPSDATDAHGGPLLLFSNPDCLVSLSRRTEEMPFYVR
                     HVDGDLLSFVHQGSGLVETEFGPLRYREGDWIYLPKACTWRQVPDAETTLLMVQATDE
                     FRVPPPGQLGRHFPFDPSQATIPEPAPIDDDGRDEYEVQLVHDGGPTSLYYQHNPIDV
                     EGWRGDNFAFTFNIADYNVVTSDSVHLPPTVHLFMQATSVYVMNFLPKPAESVPGTER
                     TPWYHRNVDYDEIAFFHGGSLYGINMPPGLISHAPQGVHHGAPEKARERARRKFEEYS
                     RVDWQVIAIDTRRRLVPSAEVLANDLGQH"
     gene            complement(4287..4676)
                     /locus_tag="VFC01_15585"
     CDS             complement(4287..4676)
                     /locus_tag="VFC01_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HZN81087.1"
                     /translation="MADDARDRITNFLQKRVANPLMRLMPFQTLLETTGRTSGQPRRT
                     PLGGSRVGDQFWFVSEFGEKSQYVKNIKANPNVRVRLRGRWHNGVAHLVPDDDPHERL
                     QSLPQMNSFAVRTFGTNLLTIRVDLTD"
     gene            complement(4779..>5340)
                     /locus_tag="VFC01_15590"
     CDS             complement(4779..>5340)
                     /locus_tag="VFC01_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN81088.1"
                     /translation="RARATTNNRLSVDDWLQAGFAILAEEGIKALKIDRLCNRLTVTK
                     GSFYWHFTDIAAYRAALVQAWGELRGDDRRDLGELAGLPPRERLSEMMSSLVSPRHWT
                     LERAMREWARTDDAAAASVRAADLRVLAAVRQAYLDYGFDAEQAELRAGATFAAGVGF
                     LHLAGPHPSARAAAGRDGFLEIMLKP"
BASE COUNT          866 a         1710 c         1791 g          973 t
ORIGIN      
        1 ggggcgaacg ggcagccgcc aaggccaccg acggacgcgt ccagccgcgt gatccccgcg
       61 cagacggcgg cgtacgcact tgccagcccg gccccgcggg tgttgtggaa gtgcgcgccc
      121 agcgggatgt cgccgatcag cgggcggatc cgggcgatca gatcgctgac gcgacgcggt
      181 gtagtggtgc cgatggtgtc ggcgatagcg agccggtcga catccgctgc ggtcgcggcg
      241 gtggtgactt cgagaacccg ctggggtggt gtcggcccgt cgaacgggca gtcccacgcg
      301 gtggcgatga tgacctcgac cgagactccg ctgtcgtggg cgattgcgac tatctctggt
      361 atcagtgccg tcgactccgc ggtggagcgc ccgacgttcg accggctatg cccgtcggcg
      421 gccgaaacca cgtattcaat tgaccctatc cccgccgcga tcgcccgttt ggcgccgttg
      481 ggactggcga ccagcgcgga gaactcgatg ccaccgaagt tgtgcagttt ggccgccagt
      541 tcggccgcat ccgccagtgc gggcaccttc gagggtgaga cgaaggccgt cgcctccatt
      601 tctcgcactc ccgtggctgc caccgccgtc agcaattcga gcttggcgga caacggaatt
      661 ggctcctcga tctgcagccc gtcgcgcagc gagacgtcgc gaatgtcgac gtgtgtcggt
      721 aggccggtca tagcacgccc tcctcgcgca gcgctgcaag ctcctcaaca ctcttgccca
      781 gtagcccgtg gtagatctcg tcgttgtgct ggcccggtcg ggccggaccg gcgttgcgga
      841 tcgttcccgg ggtttccgac agcacgggca ccacacccgg acccttgacg ttgcgcccga
      901 ttcgttcgtc gtagtggtcg gcgatcatgc cgcgggccaa cagctgcgga tccctgacga
      961 cttcggcgac ggtgttgatc ggtccgctga tcaccccggc atcggacagt gtggcgatga
     1021 tgtcggcagg gtgccgttcg gcggcccatg cgccgatgat cttgtccagc tcgtcctgat
     1081 tgcggccccg ggcaacatga ttggcgaatc ggtcgtcggt cgcgaggtcc ggctgaccca
     1141 tcgccgcgca cagcctgcgg aacaccgtgt cctggttggc ggcgatcacc acccagctgc
     1201 cgtcggccgt gggatagatg ttcgacggcg cgatccgctc caggcgggtg cccgacgggc
     1261 cacgcactac gccgccgacg tcgtagtccg ggatcgtgga ttcctggacg gccaaacagg
     1321 attcggtcag tgcggcatcg accacctggc cctcaccggt gaccgtgcgt cgatacagcg
     1381 cggcgagcgc accctgggcg gcgaacatgc ccgccaggct gtcgccgagc gagagcgcca
     1441 accgtggcgg cgggccaccg gggaagccgt tcatgtgacg cagcccgctc gcggcctcag
     1501 ccaccgacgc gtagccggcc ttgtgtgcct cgggaccggt ctggccgtag cccgagacac
     1561 ggaccaagat gatgccgcgg ttgcgttcgc gcagcacgtc atagccgaga ttccacttct
     1621 ccagtgtgcc cggccggaag ttctcgacaa tgatgtcgga cttctccacc agatcaagaa
     1681 acagctcccg gcccgcctcc acgcgcaggt tcagcgtcac cgccttcttg ttgcgcgcgt
     1741 gcaccgtcca gaagaagtga tggccgtcga gctcggcttg cccccatgtc cgcaacgggt
     1801 cgggcgcccc tggcggctcg atcttgatga cctctgcgcc catatcgccg agcagccgcc
     1861 cgccgaacgg tcccgagatc agcgtgccga cctcaatgac tcggatgccg tcgagcggtc
     1921 cggtgacagt cataacgaga acccgtgtct gtgtagccaa tccgtgcaga tgccgaccgc
     1981 ctgccgcagc gtgtcgcgct ggtccggtcc ggagtagtag tggttggcgc cgggaatttc
     2041 gtacatctcc ttatcaggat gcccgatcgc ctcgaacagc cggcgggtat ggctaggagt
     2101 acaggcgttg tcggccaggt tgccgatcac caacgtgggt accttgattt cgggaccggc
     2161 tttgacggca tcgccgttgg catcgtcata actccactgc gagagccagc tgcgtaacgt
     2221 gcagaaccgg gcgaggccga acgggcccat gttgactacc tgcgggtcgc ccagataggt
     2281 ggtccccggg gtgcggtcgt tcgggtcgac ggcggggtcc agccaacggg gatcggccat
     2341 ggtgccgtgc acgacgaatg cgaactcgtc gtcgggccgg ccgtcagctg cgagcgtcgc
     2401 tagtttttct ttgacccact tggtgattcg tcggttgcgt gcgatctggg ctgcgcggta
     2461 gcggtccagg aactcggcgg tgtagggcgg ctggttcggg ttctccgggt cgtagaggtc
     2521 caaatcgggg tcacgcttgg acgggtcggt ttcgtcgacg atcgacgcgt ccagccattc
     2581 ggtgagcgtg ccatggcggc tgatgtgcgc ggcaagcatc atgatgccgt cggcggggat
     2641 gagtcccagt ttggtcaggt ccggcccgtc gcctgacggg ctcgcagtga tcgtcgggtg
     2701 ctgcgcctgc tgctgataga acaccgacag cgagccgccg ccgctccagc cggccagtac
     2761 taccttttcg tacccaagcc ggttcttggc gtccttgatg cactcgccca gatcctcgac
     2821 gaccttctcc atcagcaacg cggagtcagt gccgcggaac cggctgttgc agtagatgac
     2881 atggtgtcca gcccgggcca gcgcgttgat catcggcagg taggcgccgc cgccgattgg
     2941 gtgcatgaac accaggacgg tgtctgatgt cttggtcttc ggcttgagta ggtagctctc
     3001 caggacgatc agttcggaga cgccaccgta gacgtcgcgc acacccgaga cattctcgta
     3061 cgcaatgaga tacgggatgc ggtcgtactc gtacttgacc gttgattcgg tagccgtcat
     3121 cagtgttgcc ccaaatcgtt ggcgagtacc tcagcggacg ggaccagtct gcgacgggtg
     3181 tcaatggcga tgacctgcca gtcgacgcgg gagtactcct cgaactttcg ccgtgcccgt
     3241 tcacgcgctt tctccggcgc gccgtggtgt acgccttgcg gcgcatggga tatcaggccc
     3301 ggaggcatgt tgatgccgta gagcgaaccg ccgtggaaga acgcgatctc gtcgtaatcg
     3361 acattgcggt gataccacgg ggtgcgctcg gtaccgggca cggattccgc gggtttgggc
     3421 aggaaattca tcacgtagac gctggtggct tgcatgaaca gatgcacggt cggcggtagg
     3481 tgcacgctgt cggacgtcac cacgttgtag tcggcgatgt tgaaggtgaa cgcgaagttg
     3541 tcgccgcgcc acccttcgac atcgatcgga ttatgttggt agtaaagcga tgtcggacca
     3601 ccatcatgga cgagctggac ctcgtactcg tcgcggccat cgtcgtcgat gggtgccggc
     3661 tcgggaatcg tggcctgcga cggatcgaac gggaagtgcc tgccgagctg tcccggcggc
     3721 ggcacacgga actcgtcggt cgcctggacc atcagcagcg tggtctcggc atcgggcacc
     3781 tgacgccagg tgcacgcctt cggcaggtag atccagtcgc cctcgcggta gcgcagtggc
     3841 ccgaactcgg tttccaccag gccggagccc tggtgcacga agctcaacag atcgccgtcg
     3901 acgtggcgca cgtagaacgg catctcctcg gtgcgccgtg acagcgacac caggcagtcg
     3961 gggttggaga acagcagcag cggtccgccg tgcgcgtcgg tcgcgtcgga cggcttgagc
     4021 tcgctggcca acacgtcgat cggtcgcagc ggtccggaac agcgataggc cgtcggatcg
     4081 tggcgccggt acatgttcgc cgtccggccg gtgaacccgc cacggccgag ttcgtcgtcc
     4141 ttcagcccgt ccaggtcggc gtgcaaacgc ttgggcgtac ggccctttcg caagtggacg
     4201 aacgattcca tggcgactcc attgacagcg gcggcgggag gggaaataga actaaaagtg
     4261 agattagttt tacttccgca ctgcggtcaa tccgtcagat cgacccggat cgtcaacagg
     4321 ttcgtgccga aggtgcggac ggcgaaactg ttcatctgcg gcagcgactg cagccgttcg
     4381 tgcgggtcgt cgtcgggcac caagtgggcg acaccgttgt gccaccggcc gcgcaaccgc
     4441 acccggacgt tggggttggc cttgatgttc ttgacgtact gcgacttctc accgaattcc
     4501 gagacgaacc agaactgatc gccgacccgg ctgccgccca gcggagtccg acggggctga
     4561 ccggacgtcc gtcccgtcgt ctccagcagg gtttgaaacg gcatcagccg catcagcggg
     4621 ttggccaccc gcttttgaag gaagttggta atgcggtcac gtgcgtcgtc ggccatcggg
     4681 gctcctctca caccatcgca gtcagaccgg cgcccagact gcggccagat cgtgttctgg
     4741 cgacctttcg cgatctcacc gcagtctcac ggccgctgtc agggcttcag catgatctcg
     4801 aggaagccgt cgcggcctgc ggccgcgcgt gcgctggggt gcgggcctgc gaggtgcagg
     4861 aagccgacgc ccgcagcgaa ggtggcgccg gcgcgcagct cggcctgctc agcgtcgaag
     4921 ccgtaatcca ggtacgcctg gcgcaccgca gcgagcacgc gaagatcggc ggcgcgcacg
     4981 ctggccgcgg cggcgtcgtc ggtgcgagcc cactcccgca tcgcccgctc cagcgtccaa
     5041 tgacgggggc tgaccagtga ggacatcatt tccgagaggc gttcccgcgg tggcagcccg
     5101 gccagctcgc cgagatcgcg ccgatcgtcg ccgcgcaact cgccccaggc ctgcacgagc
     5161 gcggcgcgat aggccgcgat gtcggtgaaa tgccagtaaa agctgccctt ggtcactgta
     5221 aggcgattgc acagccggtc gatcttgagc gccttgatgc cctcttcggc cagaatcgca
     5281 aagccagcct gcagccagtc gtcgaccgat aaccggttgt tggtggtggc ccgcgccctc
//
LOCUS       DASRJA010000352         7565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2298446,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000352 DASRJA010000000
VERSION     DASRJA010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2298446"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2288
                     /locus_tag="VFC01_15595"
     CDS             <1..2288
                     /locus_tag="VFC01_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN81089.1"
                     /translation="SPSAGRHWIVGTASARSVPLGVFAGIASDFGPDPLRRVREVIDG
                     LIGDARDGAVVIGVDDAHLLDDLSAFAIHQLVARRLATVILTIRSGESPPDAITAIWK
                     DHHLERLELQPLSLGETAQLVEQVLDGPVQSLTARRLWQFTQGNVLYLRHLVESEVNA
                     NRIAKHAGVWVWDGQPALSPTLAEFLEAQIGQAPMQVRGVLDVLAVSEPLDADILATL
                     TGPEAVVEAESLGLVRVDSGVRPAAVRLAHPMFGEVRRNASLHLRRLRGRIVTELLRR
                     PRTDPRDLVRLAALSIESDLAPDAELLLAAASAAMQLLDLQLAETLAERAVACGGGLD
                     AQIAHATITWQERGMDAESILAELADATSGPAQNQVAILRATNFAAVLGQAANAEREL
                     DVLPADDDEAQVIASALRALIDLTRGHADAAVERATAVLANPPDDAVAQMLSIWTVVT
                     GLGDLGRIDEIEPVARRGYVLADSAAEVSHLRLALAFLHAYAYRLGGALTKSDAVIAQ
                     IRSDTCDVPFEGAWHAFLAGLSTMGRGALADTQRLCQESIAYLVGDDSGRMLKGFGRT
                     WLAAVTALAGKAVDARREFAAIQWWDQDADACEWDSEKSIAQAWVCAAEGAVSQAISI
                     TRDAAQRERELGRAAVEVCLLQTATQLGDHTTAARLAELADRVQGPRAPAAAAHAGAL
                     AAGSGDALLDASRQYEEFGDRIAAADAAAQAVLAYQLAGRRGGAMSASATAQRLAAEC
                     QGAHTPALRAAATTSQLFTAR"
     gene            complement(2452..3822)
                     /locus_tag="VFC01_15600"
     CDS             complement(2452..3822)
                     /locus_tag="VFC01_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN81090.1"
                     /translation="MNPATTETVDVSALPLAPKNPLPLRRLVKLVRSLDTGQEVIRDA
                     GGPITRIQFGPKWLYPPIVAVTCPDGIRDVLGRTDDVSERCIIHEEVRHMAGDSLFVL
                     PTESWLPRKRASQPVFTKPNVRNFGGQMSRAAEAFVVRWRDGGAVDLDVECRRLTMQS
                     LGRSVLGVDLNERADVIADHMHVASAYTADRALRPVRSPRWLPTPARRRARAAVTSMR
                     AVTNEMLQACRADPTLDAPLVQALIAATDPQTGRSLSDDDICNDLLIFMLAGHDTTAT
                     ALTYALWALGHHRDIQDRVAAEAAEIGDRELTPQDVPRLGYTVQVLREALRLQPPAAG
                     VARLAMRDMAVGGYRVEAGSLMAVGIYALHRDPALWDRPLVFDPDRFSPENSNDRDRW
                     QFIPFIAGPRSCIGEHFAMLETTLAMATVVRSLEIRSVDEDFPVAVPFTTVADGPIRA
                     RLAPRR"
     gene            complement(3819..4304)
                     /locus_tag="VFC01_15605"
     CDS             complement(3819..4304)
                     /locus_tag="VFC01_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZN81091.1"
                     /translation="MTPLERIAPAFIEMAHSIVWASVATVDENGRPRTRILHPIWEWD
                     GTDLFGWIATVPSPVKKAHLAVHPEMAVSYWTTTHDTCTAECLVEWYHDDETRAAVWQ
                     KFATGPAPVGYDPSIIPMWADGPTSDQFAALRLRPYRLRVMPGTVMMKGEGETLTWTV
                     M"
     gene            complement(4358..4657)
                     /locus_tag="VFC01_15610"
     CDS             complement(4358..4657)
                     /locus_tag="VFC01_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81092.1"
                     /translation="MGTEEQQQNQAGEQQQTQSGQITEKPEVTDEHKEKAREIAKAYD
                     DDLQTTTLPGSGGTVSGTAVTDWVDEKDKGNIETTAEEGKVEFRKTEEFKKKLEE"
     gene            complement(4702..5298)
                     /locus_tag="VFC01_15615"
     CDS             complement(4702..5298)
                     /locus_tag="VFC01_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN81093.1"
                     /translation="MHGADPTLEAILDAAVVEFERHGFRRVALDDVARRARVSRTTIY
                     RRFANKDELVAAVIERENVRLFADIALELKQAGPQANYYVEAFTLSILKFRRHRVLDR
                     MMTDEPGLVLELAGRHYAAAIERMAEALRVIFPTGFAERIGEQAVTELADTILRYAAM
                     ALLLPSAQPLETADDIRAFATQHFLPSLPIALRAVPAG"
     gene            complement(5285..6142)
                     /locus_tag="VFC01_15620"
     CDS             complement(5285..6142)
                     /locus_tag="VFC01_15620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZN81094.1"
                     /translation="MTEVAERRERTDDALPLGPQSLVWRYFGDNRMYLIGPRPAVLQN
                     MLAELGQGVLDHSTFFADTAERLRRTIPPIFNTVYGSDGDNAGTQVRDFHHHVKGDMP
                     DGGRYHALDPDTYFWAHATFVEQVLYFADTFVKRLTRTEKEQIYLESKTWYRRYGVSD
                     RPMPANYAEFERYWDRMMDEIVVAHPTAKYGVGYVTKGFPRPKAVSPALWRLIAPVFN
                     PVAAFLTTGGLPPRARDLLGLRWSDRQERRYQRFAAFWRSRPVNWVWDHLPMRLRYNG
                     YALKGYARG"
     gene            complement(6272..6496)
                     /locus_tag="VFC01_15625"
     CDS             complement(6272..6496)
                     /locus_tag="VFC01_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81095.1"
                     /translation="MRARVELVLAVIAAAGCVLSWLAASSTVVVAPVLKGEPQTTSVD
                     YSAPLLVLSLVLAAVAGVLVVLGIVHLRRG"
     gene            complement(6493..6984)
                     /locus_tag="VFC01_15630"
     CDS             complement(6493..6984)
                     /locus_tag="VFC01_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 domain-containing protein"
                     /protein_id="HZN81096.1"
                     /translation="MTDTGPPPEVLFYEQGASWWWLLAGPGAGIAMALIQLSAGYGIQ
                     LVVPGIFFVLVGGFLAIQVKAARIHTSVELTPDHLRQGTERVRIDDIVRIYPEPKGED
                     EPKWMSSRALGELTGVPRGRTGIGLKLTNDRGAQAWARKHRQLREALTHLVEERIPPE
                     PAS"
     gene            complement(6981..7364)
                     /locus_tag="VFC01_15635"
     CDS             complement(6981..7364)
                     /locus_tag="VFC01_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81097.1"
                     /translation="MTAPQSRPRVVTAAFWCWVVASLLLIVGGLIAASVSALPMVYRG
                     GGVITVLAGAGMAFLAGRTRTSGDTRFRRAGIALSLAIVVLVAILAVIGVVHMLTLLA
                     VLPLIAGTVLITRPSAASPSEQEPQ"
     gene            complement(7385..>7565)
                     /locus_tag="VFC01_15640"
     CDS             complement(7385..>7565)
                     /locus_tag="VFC01_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81098.1"
                     /translation="LLTGGGVASVVYAATNLFTVARPPVAAVAYAVTAIVGSVLIAGG
                     VFLLHRKDAHAFFTR"
BASE COUNT         1303 a         2492 c         2513 g         1257 t
ORIGIN      
        1 gttcgcccag cgccggtagg cattggatcg tcggaacggc atcggcacgc agcgttccct
       61 tgggcgtatt cgccggcatc gccagcgact tcggccccga cccgctgaga cgagtacgag
      121 aggtgatcga cgggctgatc ggcgacgccc gcgacggcgc cgtggtgatc ggtgttgacg
      181 atgcgcatct cttggatgac ttgtcggcgt tcgcgattca ccaactcgtc gcccgccgcc
      241 tagcgaccgt gatcctgacg attcggtccg gcgaatcgcc gccagacgcc atcaccgcga
      301 tctggaagga ccaccacctg gaacgccttg agctgcaacc gctgtcgctc ggcgagaccg
      361 cgcaactggt cgaacaggtg ctggacggtc cagtgcaatc ccttaccgca cggcggctct
      421 ggcagttcac ccagggcaac gtgttgtatc tacgccacct tgtggaaagc gaagtgaacg
      481 ccaaccggat tgcaaagcat gcgggggtct gggtgtggga tgggcaaccc gcactgtcac
      541 cgacgctcgc tgagttcctc gaggcgcaga tcggccaggc gcccatgcaa gtccgtggtg
      601 ttctggatgt tctcgccgtg agcgaaccgc tggatgccga catattggcc accctcactg
      661 ggccggaggc ggtggtcgag gctgaatcgc tggggttggt gcgggtggac tccggcgtgc
      721 ggcccgcggc ggtgcgcttg gcgcacccca tgtttggaga ggttcgacgc aacgcttcgc
      781 tgcatctgcg gcgacttcgc gggcgcatcg tgaccgaact gctccggaga ccaaggacag
      841 atccgcgaga tcttgtgcgc ctcgcggcgc tgtccatcga atccgatttg gcgcccgacg
      901 ctgaacttct cctggccgcg gcatcggcgg caatgcagct gctggatttg cagctggcgg
      961 aaaccctcgc cgagcgcgcc gtcgcctgcg gcggcggact cgatgcgcag atcgctcacg
     1021 cgacgattac gtggcaggag cgcggcatgg atgcggagag cattctcgcc gaactcgccg
     1081 atgcaacatc gggtccggcg cagaatcaag tcgcgatcct gcgcgccacc aacttcgccg
     1141 ccgtcctcgg tcaggccgcc aacgccgaac gcgaactcga tgtgctgccc gccgacgatg
     1201 acgaagctca ggtcatcgct agcgctctgc gggcactgat cgatctgacg cgcggtcatg
     1261 ccgacgccgc cgtggaacgg gccaccgccg tcctcgccaa tccacctgac gacgctgtgg
     1321 cgcagatgct ttcgatttgg accgtggtca ctgggctcgg cgacctcggg cgcatcgatg
     1381 aaatcgagcc cgtggcgagg cgcggctacg tcctagccga tagcgctgcg gaggtgtcgc
     1441 acctcaggct cgcgctggcg ttcctacatg catacgcgta tcgactcggc ggtgcgctga
     1501 cgaaatccga cgcggtgatc gcgcaaatcc ggagcgacac atgcgacgtt ccgttcgagg
     1561 gtgcgtggca tgccttcctt gccggcttgt cgacgatggg tcgaggtgct ctggccgaca
     1621 ctcaacggtt gtgtcaagaa tccatcgcgt acctggtcgg cgacgacagt gggcgcatgc
     1681 tgaagggatt cgggcgcaca tggctggctg cggtgaccgc cctggcaggc aaagccgtcg
     1741 acgcgcgacg cgaattcgcg gccatccagt ggtgggacca agacgccgac gcgtgcgagt
     1801 gggactccga gaaatccatc gcgcaggcct gggtgtgcgc cgccgaaggc gctgtatcgc
     1861 aggccatctc gatcacccgc gacgcggcgc agcgggaacg cgagctgggc cgagccgcgg
     1921 tggaagtgtg cctcctgcaa accgcgaccc aactgggcga ccacaccacc gctgcgcgac
     1981 tagcagagtt ggccgatcgg gtgcagggcc cccgcgcgcc cgcggcggcg gcccatgcgg
     2041 gcgcgctcgc ggcaggaagc ggcgatgcgt tattggatgc gtcacgccaa tacgaggaat
     2101 tcggcgatcg catcgcggcc gccgacgccg cagcccaagc cgtcctcgcc taccaactcg
     2161 cggggcgtcg cggcggagcg atgagcgcgt ccgccacagc ccagcggctg gcggcagaat
     2221 gccaaggcgc ccacacacct gcgttacgtg ccgcggcgac gacatcgcaa ctattcaccg
     2281 cccgttagct cgaaatcatt tcccttgccg cacaaggact atcgaataag gagatcgccg
     2341 accggctcgc catgtccgtc cgctccgtcg aagggcatct cttccgcgcc tgccaacgcg
     2401 tcggagcgaa cagccgcgac cagttgatcg ccatgctgca cgggtcgtga gttaccttcg
     2461 cggcgcgagg cgcgcccgga tcgggccgtc ggcgaccgtg gtgaacggca cggcgacagg
     2521 gaaatcctcg tcgacggagc ggatttcgag ggaacggacg acggtcgcca tcgcaagggt
     2581 cgtttcgagc atcgcgaaat gttcaccgat gcacgagcgg ggtcctgcga tgaacgggat
     2641 gaattgccag cggtcgcgat cgttggagtt ttccgggctg aatcggtcgg gatcgaaaac
     2701 caatgggcgg tcccacaggg cgggatcgcg gtgcaaagca tagattccga cggccatcag
     2761 gctgccggcc tcgacgcggt acccgccgac cgccatgtcg cgcatcgcca gccgcgcgac
     2821 gcccgccgcg ggcggctgca gccgcagcgc ctcgcgcaac acctggacgg tgtatccaag
     2881 ccgcggcaca tcctgtggcg tcagctcccg atcgccgatc tcggcggcct ccgccgccac
     2941 gcggtcctgg atgtcgcgat ggtgtcccag ggcccacagc gcgtaggtga gcgcggtcgc
     3001 cgtcgtatcg tggccggcca gcatgaagat cagcaagtcg ttgcagatgt cgtcgtcgga
     3061 gagcgaccgg ccggtttgcg gatcggtggc cgcgatcaac gcctgaacga gcggcgcgtc
     3121 cagggtcgga tcggcacggc acgcctgcag catctcgttt gtcaccgcgc gcatcgaggt
     3181 aaccgccgca cgggcgcggc ggcgggccgg ggtcggtagc catcgcggtg aacgcacggg
     3241 ccgaagcgcg cgatcggcgg tgtaggcgga cgcgacatgc atgtggtcgg cgatgacatc
     3301 agcgcgttcg ttgagatcga caccaagcac cgagcggccc agcgactgca tggtcagccg
     3361 tctgcattcc acgtcgaggt cgacagcgcc gccatcgcgc cagcgaacga cgaaagcctc
     3421 tgcggcccgg gacatttggc cgccgaaatt gcgaacgttc ggtttcgtga agacgggttg
     3481 cgatgcccgc ttgcggggca gccacgactc ggtgggcaag acgaacaggc tgtcccccgc
     3541 catatggcgg acttcttcgt ggatgatgca ccgctccgag acgtcgtcgg ttcgacccaa
     3601 tacgtcgcgg atcccgtcag ggcacgtgac cgccacgatc ggcggataca gccacttggg
     3661 cccgaactga atacgggtga tcggaccgcc ggcgtcgcgt atcacttcct gcccggtgtc
     3721 caggctgcgc accagcttca ccaaccgccg aagcggcagc ggatttttcg gcgccaacgg
     3781 cagagcgctg acgtcgaccg tctcagtggt cgccgggttc acatcacggt ccaggtgagg
     3841 gtttcgcctt cacctttcat catcaccgtg ccgggcatta cgcgcagccg atatgggcgc
     3901 aggcgcagcg cggcgaactg atccgatgtc gggccgtccg cccacatcgg gatgatcgac
     3961 gggtcgtagc cgaccggcgc cggaccggtc gcgaacttct gccacacggc ggcccgggtc
     4021 tcgtcgtcgt gataccactc caccaggcac tccgcggtgc aagtgtcgtg ggtggtggtc
     4081 cagtagctga ccgccatttc cgggtgtacg gcgaggtgcg cctttttgac cgggctgggc
     4141 accgtggcga tccagccgaa caggtcggtg ccgtcccact cccagatcgg atgcagaatg
     4201 cgggtgcggg gccgaccgtt ttcgtcgacg gtggcaactg aggcccagac aatcgagtgg
     4261 gccatctcaa tgaacgccgg cgcgattcgc tcaagcgggg tcacgattca ctagcgtagg
     4321 cacatgccgc cgctggctcc gcgccagtgc cgcggagcta ctcttctagc ttcttcttga
     4381 attcctccgt cttgcggaat tcgaccttgc cctcctcggc ggtcgtttcg atgttgcctt
     4441 tgtccttctc gtcgacccag tcggtcacgg cggtgcctga aaccgtcccg ccgctgcccg
     4501 gcaatgtggt cgtctgaagg tcgtcgtcgt aggccttggc gatttccctg gccttttctt
     4561 tgtgctcgtc cgtcacctcg ggcttctcgg taatttggcc cgattgggtt tgttgctgtt
     4621 ctcccgcttg gttttgttgc tgttcttcgg tacccatgcc gtagggctgc ccgatggtcc
     4681 ggcgaacgaa acggagtcca gctaaccagc gggaaccgcc cgcagcgcga taggcaggct
     4741 gggcagaaag tgctgcgtcg caaacgcgcg gatatcgtct gcggtctcga ggggttgggc
     4801 gctgggcagc agcagcgcca tcgccgcgta ccgcaggatg gtgtcggcca gctcggtgac
     4861 cgcctgctcc ccgatgcgct cggcgaagcc ggtcgggaag atcacccgca acgcctcggc
     4921 catccgctcg atcgcggcgg cgtaatgccg tccggccagc tcgagcacca ggccgggctc
     4981 gtcggtcatc atccgatcga gcacccggtg ccgacgaaat ttcaggattg acagtgtaaa
     5041 cgcctcgaca tagtaattcg cttgcggccc agcctgtttc agctctagag cgatgtcggc
     5101 gaacagccgg acgttctccc tctcgatgac cgccgcgacg agctcatcct tgttggcgaa
     5161 ccgccggtag atggtcgtgc ggctgacccg cgcccgacgc gcgacatcgt cgagggccac
     5221 ccgccgaaag ccatgccgtt cgaactcgac gacggcggcg tcgaggattg cttccagggt
     5281 gggatcagcc ccgtgcatag ccctttaggg catagccgtt gtagcgcagc cgcattggca
     5341 gatgatccca cacccaattc acgggtcgcg aacgccagaa tgctgcgaag cgctgatagc
     5401 gacgctcctg gcggtcgctc cagcgcagac cgagaaggtc gcgggcgcgc ggcggcaggc
     5461 cgcctgtcgt caggaatgcg gccaccggat tgaacacggg cgcgatgagt cgccacagcg
     5521 ccggcgacac cgccttcggg cgggggaagc ccttcgtcac atagccgacg ccgtacttgg
     5581 cggtcgggtg cgcgacgacg atctcgtcca tcatccggtc ccaatagcgt tcgaactcag
     5641 cgtaattcgc cggcatcggc cggtcactaa caccgtagcg gcggtaccag gtcttggatt
     5701 cgaggtagat ctgttccttc tcggtccgtg tcagccgctt gacgaatgtg tcggcgaagt
     5761 agagcacctg ctcgacgaag gtcgcgtgcg cccagaagta cgtgtcgggg tcgagcgcgt
     5821 gatagcggcc gccgtccggc atatcgccct tgacgtggtg gtggaagtcg cgcacctggg
     5881 tgccggcgtt gtcgccgtcg gagccgtaca ccgtgttgaa gatcggcggg atcgtgcgcc
     5941 tgagccgctc ggcggtgtcg gcgaaaaagg tcgagtggtc cagcaccccc tgaccgagct
     6001 cggcgagcat gttctgcagc accgccgggc gtgggccgat gagatacatc cgattgtcgc
     6061 cgaagtagcg ccagaccagc gattgcgggc cgagcggcaa cgcgtcgtcg gtgcgttcgc
     6121 ggcgttccgc aacctccgtc atgtggaaca gtgttacaga tttcgaactt tgtaccaacc
     6181 gcgtgtgagc ggtctcacgg atcgcgtcga gactgcaacc acgcagagaa agttcgagag
     6241 ggggcctgct ggaataccgt ttcggcgaaa attagccgcg ccgcaggtga acgatcccga
     6301 gcacgaccag caccccggcg accgccgcca acaccagcga cagcaccagc agtggcgcgc
     6361 tgtagtccac cgacgtcgtc tggggctcgc ccttgagcac cggcgcgacg accaccgtgg
     6421 agctggcggc cagccagctc agcacgcagc cggccgccgc gatgacggcc agcaccagct
     6481 cgacacgggc cctcatgacg ccggctccgg cggaatgcgc tcttcgacga ggtgggtcag
     6541 ggcctcccgc aactggcggt gcttgcgggc ccacgcctga gcaccgcgat cattggtcaa
     6601 cttcagcccg atgccggtgc gcccgcgcgg taccccggtg agctcaccca gtgcgcggga
     6661 tgacatccac ttcggctcgt cctccccctt cggctcgggg taaatccgca cgatgtcgtc
     6721 gatgcggacc cgttccgtgc cctggcgcag gtgatcgggt gtcaactcca ccgacgtgtg
     6781 gatgcgtgcg gccttgacct gaatagcgag gaaaccgccg accagcacga agaagatccc
     6841 gggaaccacc agctgaatgc cgtagccggc cgacagctgg atcagcgcca tggcgatacc
     6901 cgccccgggt cctgccaaca gccaccacca gctggcaccc tgctcataga acaacacctc
     6961 gggtggcggg cccgtatcag tcactgcggc tcctgctcgg acggcgacgc agccgagggt
     7021 cgggtgatca gcacggttcc tgcgatcagc gggagcacgg ctagcagcgt gagcatatgt
     7081 accacgccga tcaccgcaag aatggcgacg agcacgacga tcgccagcga cagggcgatc
     7141 ccggcgcggc gaaagcgcgt atcgccgctg gtgcgggtgc ggcccgccaa gaacgccatc
     7201 ccggcgccgg cgagcaccgt gatgacgccc cctcctcggt acaccatggg aagtgcagac
     7261 accgacgcgg caatcagacc accgacgatc agcagtaacg aggcgaccac ccagcaccaa
     7321 aatgcggcgg tgacaacacg cggtcgcgac tgtggggctg tcatgtcccg gccagcctag
     7381 cggctcacct agtgaagaag gcgtgggcgt ccttgcgatg cagcaggaac acaccccccg
     7441 cgatcagcac cgacccgaca atcgccgtga cggcgtaggc gacggccgca acaggggggc
     7501 gggccacggt gaacaggttg gtggccgcgt agaccaccga cgcgacgccg ccgcccgtca
     7561 acagg
//
LOCUS       DASRJA010000353         7262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2854257,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000353 DASRJA010000000
VERSION     DASRJA010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2854257"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VFC01_15645"
     CDS             <1..838
                     /locus_tag="VFC01_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HZN81099.1"
                     /translation="SVLGLPEFDPLVREEAELADLDITRYVHSNRYVDALEEARPDAG
                     YVYLDGGDTMMEPSTWEVVLRGVGATVRAVDMVLTGEVQNAFVACRPPGHHAETERAM
                     GFCLFNNISIGARHAQRNHGLQRVAIVDFDVHHGNGTQEIFYSDPSVLYASTHQMPLF
                     PGTGAVRETGVGNIFNSPLAAGDGGAELREAFEDRILPALDTFRPELIIVSAGFDAHE
                     RDPLGSLQMTADDFAWVTRALMESAEKNCEGRLVAVLEGGYDLQGLADSVSAHVAELM
                     KG"
     gene            complement(835..2274)
                     /locus_tag="VFC01_15650"
     CDS             complement(835..2274)
                     /locus_tag="VFC01_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZN81100.1"
                     /translation="MSIEMGARTLDVDLLARELGNWRTSSHSGPAYQGLADGIQMLII
                     DGRLPVGARLPSERALADTLRVSRTTVTAAYTQLREDGYLNARRGARSTTALPVAPSA
                     SPADAPARVNLAHAALSAPAAVRDAYIEATHEMTPYLHDIGIDLTGLPPLREAIAERY
                     CARGLPTDPDEVMVTTGALHAIGLVLAAYVQPGDRVLVEQPTYHGVLSAITATGARAV
                     PVAMAHHGWELDAVQAAIRQLAPSLAYLIPDNQNPTGLTMSTSDRKRLAQIISDTRTR
                     TIIDETMVDVWLDEPVPAPTAAALSTRRDLVMSIGSMSKSFWGGLRIGWIRAERGTLA
                     TIAAVRPSVDMGTPIVEQLVAANLLHRPDDVLPERRELLRARRALLTSLLSQQLPDFQ
                     PGPGCGGMALWVQLPSPMSSALSAAASRLGLDLPPGPRFGVDGTLERFVRIPYTLPDD
                     QLAEAVELLARAWRTVTGATAAEPSAVVV"
     gene            2338..2490
                     /locus_tag="VFC01_15655"
     CDS             2338..2490
                     /locus_tag="VFC01_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81101.1"
                     /translation="MSMNWTSQLARPLARLFQPADTIPAEFVDRDTERVRRELELIRI
                     RFPHHA"
     gene            2483..3148
                     /locus_tag="VFC01_15660"
     CDS             2483..3148
                     /locus_tag="VFC01_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81102.1"
                     /translation="MRKATLRGLALLVGLSGYGFSMAVMVRAGLGLDPWDVFHQGLAR
                     HTGMTIGVASAVVGVAVLLAWIPLRNRPGIGTIANVIVIAITVDAGLWLLPTPTSLPV
                     RIAMMVSAVVLNAFSTVLYIGAALGPGPRDGLMTGLVVRTGLSVRLVRTSIEATVLAV
                     GWLLGGTVGVGTVLYAFGIGPLVQLFLRLTPQRVLAVSGWASVAEASDLDGNRESGEL
                     SHA"
     gene            3166..3819
                     /locus_tag="VFC01_15665"
     CDS             3166..3819
                     /locus_tag="VFC01_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HZN81103.1"
                     /translation="MSMTDLLARDAIALAGETSRAILGIAGSPGAGKSTLVEELLERV
                     RAAKGADWVAHVPMDGFHLADAQLDRIGARGRKGAPDTFDALGYAHLLERLRLEADEP
                     VYVPGFDRTLEQPLAAALVVMPSARLVVTEGNYLLLDDPHWARARRAMDAVWFVASEE
                     TMRVERLVARHVQFGKSPHEARAWVATTDQRNADLVAGTVGRADRVIVNGAGGWGLG"
     gene            3851..4693
                     /locus_tag="VFC01_15670"
     CDS             3851..4693
                     /locus_tag="VFC01_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN81104.1"
                     /translation="MADAELDAADPDLLIDFAKVTLRRGRQTLVGPVTWSVELDERWV
                     VIGPNGAGKTSLLRIAAALEHPSSGTAFVLGERLGRVDMSELRSRVGLSSSALSQRVP
                     DGEVVRDLVVSAGYAVLGRWREKYEDVDYSQAIDMLESVGAEHLAERSYGTLSEGERK
                     RVLIARSMMTDPELLLLDEPAAGLDLGGREELLARLEDLANDPDSPALVLVTHHVEEI
                     PRGFSHCLILSEGKVVDSGLLSDTLTAENLSKAFGQSIALDVVDGRYFARRTRTRAAH
                     RRRE"
     gene            4690..5520
                     /locus_tag="VFC01_15675"
     CDS             4690..5520
                     /locus_tag="VFC01_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZN81105.1"
                     /translation="MTDPLVPRPAATVMLVRDTANGIKVFLMRRHSAMDFVAGVMVFP
                     GGGVDDRDRNADIAWFGPEPDWWAERLGVEPGLAEALVCAAARETFEESGVLFAGPAD
                     DPDGIVGDASIYRESRLALADKSLSFGEFLRKENLVLRADLLRPWANWVTPKEERTRR
                     YDTYFFVGALPEGQRADGDNTETDRAFWTTPQAALDEFADGTTFLLPPTWTQLDSLNG
                     RTVAEVLAVERQIVAVEPHLAANKDTGNWEIEFFNSDRYNAARNRRAPDGYGPDTPIA
                     "
     gene            5517..6164
                     /locus_tag="VFC01_15680"
     CDS             5517..6164
                     /locus_tag="VFC01_15680"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZN81106.1"
                     /translation="MNEFVSVHTSDEQPGIATLLLSRPPTNALTRQVYREIVSAAADL
                     GQHDDISAVIVFGGHEIFSAGDDMPELSTLNADEADVAARVCREAVDALAAIPKPTVA
                     AITGYTLGGGLNVALAADWRVAGDNVKFGATEILAGRVPQGGTDRLARTTGESKAKDL
                     VFSGRFIDAKEALALGLIDEMVAPDGVYDAALAWARRFLDYPPQALAAAKATFAV"
     gene            6176..7180
                     /locus_tag="VFC01_15685"
     CDS             6176..7180
                     /locus_tag="VFC01_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN81107.1"
                     /translation="MSFEETDTPPDVADQAAPVPNPHATAEEVEAARHDSKLAQVLYH
                     DWEAESYDEKWSISYDKRCVDYARNLFDATVPDEELRELPYGRALELGCGSGFFLLNL
                     IQAGVARRGSVTDLSPGMVKVATRNGENLGLDVDGRVADAEGIPYDDDTFDLVVGHAV
                     LHHIPDVELSLREVVRVLKPGGRFVFAGEPTNAGENYARPLSTLTWRAVTNVTKLPGL
                     GGWRRPQAELDESSRAAALEAIVDLHTFAPGDLERMARSAGAVEVSTATTEFTAAMLG
                     WPLRTLEAAVPPGKLGWGYAKFAFHSWITLSWVDHNLWGRVVPKSWYYNVMVTGVKPS
                     "
BASE COUNT         1158 a         2361 c         2544 g         1199 t
ORIGIN      
        1 gtcggtgctc ggcctgcccg agttcgatcc gttggtgcgc gaggaggccg agctcgccga
       61 cctcgatata acgcggtacg tgcattcgaa ccgatacgtc gatgcgctgg aagaggctcg
      121 cccggacgcc ggttatgtct atctcgacgg cggcgacacc atgatggagc cgtcgacgtg
      181 ggaggtcgtg ctgcgcggtg tcggtgcgac cgtgcgagcg gtcgatatgg tgctcaccgg
      241 ggaagtgcag aacgccttcg tcgcatgccg tccgccgggc caccacgccg agaccgaacg
      301 ggcgatgggg ttctgcttgt tcaacaacat cagcatcggc gcgcgtcatg cgcaacgcaa
      361 tcacgggctg cagcgggtcg cgatcgtcga tttcgacgtt catcacggca acggcacgca
      421 ggagatcttc tactcggatc cgagtgtgct ctacgcgtca acgcatcaga tgccgttgtt
      481 ccccggcacc ggcgcggtcc gcgaaacggg tgtgggcaat atcttcaact cgccccttgc
      541 agccggtgac ggcggcgccg aacttcgtga agccttcgag gaccgaatct tgcccgcgct
      601 tgacacgttc aggccggagc tcatcatcgt ctcggccggc ttcgatgccc atgagcgcga
      661 tccgctcggt tcgttgcaga tgacggccga cgatttcgct tgggtgacac gcgcattgat
      721 ggagtcagcc gagaagaact gcgaaggccg tctggtggcg gtgctggaag gcggttatga
      781 ccttcagggg cttgccgatt cggtcagtgc ccacgtcgcc gaactgatga aaggctaaac
      841 gaccaccgcg gaaggttcgg ccgccgtcgc gccggtgacc gtgcgccacg cccgcgccag
      901 cagttcgacc gcctcggcca gctggtcgtc gggcagtgtg tacggaatgc gcacgaatcg
      961 ttcgagcgtg ccgtccacgc cgaagcgggg accgggcggc agatccaggc cgaggcgcga
     1021 cgcggccgct gacagcgccg aactcatcgg cgacggcagc tgaacccaca gcgccatccc
     1081 gccacatccc gggcctggct gaaagtccgg aagctgttga ctcagcagcg atgtcagcag
     1141 ggcgcggcgg gcgcgcagaa gttcgcggcg ctcgggaagg acatcgtcgg ggcgatgcag
     1201 gaggtttgcg gcgacgagtt gctcgacgat cggggtaccc atgtcgaccg acgggcgcac
     1261 cgccgcgatg gtcgccagcg tgccgcgctc cgcgcggatc cagccgatgc gcagaccgcc
     1321 ccagaacgac ttcgacatcg agccgatact catgacgagg tcgcgccgcg tcgatagggc
     1381 ggccgccgtc ggtgccggca ccggttcgtc caaccagacg tccaccatgg tttcgtcgat
     1441 gatggtgcgg gtgcgagtgt cagaaataat ctgcgccaac cgttttcggt ccgaagtcga
     1501 catggtcagg ccggtcgggt tctggttgtc ggggatcagg tacgccaggc tcggggccag
     1561 ttgcctgatg gccgcctgga cggcgtcgag ttcccagccg tggtgcgcca tggcgaccgg
     1621 aaccgctcgg gccccggtgg cggtgatggc cgacagcacg ccgtgatagg tgggttgctc
     1681 gaccagcaca cggtcaccgg gttggacgta tgcggccagg accaaaccga tggcgtgcag
     1741 cgcgcccgtg gtgaccatca cctcgtcggg atcagtggga agccctcgtg cgcaatacct
     1801 ttcggcgatc gcttcacgca gcggcggcag gccggtgagg tcgatcccga tgtcgtgcag
     1861 atagggcgtc atctcatgcg tcgcctcgat gtaggcgtcc cgcacggcgg caggcgccga
     1921 gagcgcggcg tgggcgaggt tgacccgcgc gggggcgtct gcgggcgacg cgctcggcgc
     1981 gaccggcagc gcggtggtac tgcgcgcgcc gcggcgtgcg ttgaggtagc cgtcctcgcg
     2041 cagctgtgtg tacgcagcgg tgaccgtggt gcgggagacc cgcagcgtgt cggcgagcgc
     2101 ccgctcgctg ggtagccgcg cgccgacggg caatcgcccg tcgatgatca gcatctggat
     2161 gccgtcggcc agcccttggt acgcagggcc actgtgactg gacgtgcgcc agttgcccag
     2221 ctcgcgggcc agaaggtcaa cgtcgagcgt tctggctccc atttcgatgc tcatttgcag
     2281 gccagtatgc gccaactggc tattgaggag caagccagtt ggccggatta tcgggtcatg
     2341 agcatgaatt ggacatccca actggcccgg ccactggccc ggttattcca accggcggac
     2401 accatccccg ccgaattcgt cgaccgtgac accgaacgcg tccgccggga attagagctg
     2461 atccggattc gctttccaca ccatgcgtaa agccactctg cgcgggctgg cactcctcgt
     2521 cggcctgagc ggctacggct tctcgatggc ggtgatggtc cgcgccgggc tgggcctgga
     2581 cccgtgggac gtcttccacc aaggactggc ccgccacacc ggcatgacga tcggtgtggc
     2641 gtccgcggtt gtcggcgtcg cggtgctact ggcctggatt ccgctgcgga accggccggg
     2701 catcggcacg atcgccaacg tcatcgtcat cgcgatcaca gtcgacgccg gactctggct
     2761 attgccgacc ccgacgtcgc tgccggtgcg tatcgcgatg atggtgagcg cggtggtgct
     2821 caacgcgttc agcactgtcc tctacatcgg cgccgcgttg ggccccggtc cgcgcgacgg
     2881 gctgatgacc ggactggttg tgcgcaccgg actttcggtg cggctggtgc gcacgtcgat
     2941 cgaagcgacc gtgctggccg tcggctggct cctcggcggg acggtgggcg tcggcaccgt
     3001 gttgtacgca ttcggcatcg gcccgctggt gcaactgttc ctgcgcctca cgcctcagcg
     3061 ggtgttggcg gtcagtggct gggcttccgt cgccgaagcc agcgatttgg acggaaatcg
     3121 cgagtcgggc gaactcagcc acgcgtagtg accgaaactc agcagatgtc gatgacggat
     3181 ctgctggccc gcgatgcgat cgcgctggcc ggtgagacat cgcgcgcgat cctgggcatc
     3241 gcaggcagcc cgggcgccgg gaaatcgact ctggtcgagg agttgctgga gcgggtccgt
     3301 gcggccaagg gggccgactg ggtcgcgcat gtgccgatgg acggcttcca tctggccgat
     3361 gcccagctcg accgcatcgg ggcgcgcggc cgcaagggcg cccctgacac gttcgatgcg
     3421 cttggttacg cgcatctgct cgagcgactg aggctcgagg ccgacgagcc ggtatacgtg
     3481 ccggggttcg accgcacgct cgaacagccg ctggccgcgg cgcttgtcgt aatgccgtcg
     3541 gcacgcctcg ttgtcaccga gggcaactac ctgctgctcg acgatccgca ctgggcacgg
     3601 gcccgccggg cgatggatgc cgtgtggttc gtcgccagtg aggagaccat gcgcgtggag
     3661 cgtctcgtcg cgcggcacgt ccagttcggc aagtcgccac atgaggcgcg ggcctgggtc
     3721 gcgaccaccg atcagcgcaa cgcagatctg gtggctggaa cggtcggcag ggccgaccgg
     3781 gtcatcgtca acggcgccgg aggatggggc ctcgggtagc gacacctgtc gagtacggca
     3841 cgatggtcgg gtggccgacg ccgaacttga tgcagccgac cccgacctgc tgatcgactt
     3901 cgcgaaagtc actctgcgac gcggtcgcca gaccctggtc ggacccgtca cctggtcggt
     3961 cgaactcgac gaacgctggg tggtgatcgg gccgaacggc gcgggaaaaa cgtcgctgtt
     4021 gcgcattgcg gccgccctgg aacacccgtc gtcgggcacg gcgttcgtgc tcggcgagcg
     4081 gctcggccgc gtcgacatgt ccgaactgcg ctcgcgtgtg gggctctcca gctcggcgct
     4141 gtcccagcgg gtgcccgacg gtgaagtcgt ccgcgatctg gtcgtttcgg ccggctacgc
     4201 ggtgctgggt cgctggcggg agaaatacga ggacgtcgac tacagccagg ccatcgacat
     4261 gctcgagagc gtcggcgccg agcacctcgc cgaacggagc tacggcacgc tctccgaagg
     4321 cgagcgcaag cgggtgctga tcgcccggtc gatgatgacc gaccccgagc tgctgctgct
     4381 cgacgaaccc gccgccgggc tggacctcgg cggtcgcgag gaactgctgg cgcggctcga
     4441 ggacctcgcc aacgatcccg actccccggc gctcgtgctg gtcacccacc acgtcgagga
     4501 gattccgcgc gggttctccc actgcctgat cctgtcggag ggcaaggtgg tcgattcggg
     4561 tctgctgtcg gacacgttga cggccgagaa cctgtcgaag gcgttcgggc aatccatcgc
     4621 cctcgacgtc gtcgacgggc gctacttcgc ccgtcgcacc cgcacaaggg cggcgcacag
     4681 gaggcgagaa tgacagaccc attggtgccg cggccggcgg ccactgtgat gctcgtgcgc
     4741 gacaccgcca acgggatcaa ggtgttcctg atgcgccggc actccgcaat ggatttcgtg
     4801 gcgggcgtga tggtgttccc cggcggaggc gtcgacgacc gcgaccgcaa cgccgatatc
     4861 gcgtggttcg gtccagagcc cgattggtgg gccgaacggc tgggcgtcga accgggtctc
     4921 gccgaggcgt tggtgtgtgc tgcagcccgc gagaccttcg aggagtccgg tgtgctgttc
     4981 gccgggcccg ccgacgaccc ggacggcatc gtcggcgatg catcgatcta ccgcgaatcg
     5041 cggctggcgc tcgcggacaa gtcgttgtcg ttcggggagt ttctgcgcaa ggagaacctg
     5101 gtgctgcgcg cggatctgct tcggccgtgg gcgaactggg tgacgccgaa agaggagcgc
     5161 acccgccgct acgacaccta cttcttcgtc ggggcactgc ctgaaggtca gcgcgccgac
     5221 ggcgacaaca ccgaaaccga tcgcgcgttc tggaccacgc cacaagcagc tctcgacgaa
     5281 ttcgccgacg gaacgacgtt cctgctgcct cccacctgga cgcagctgga ctcgctcaac
     5341 ggccgcacgg tcgcggaagt gcttgcggtc gaacgtcaga tcgtcgccgt cgaaccgcat
     5401 ctcgccgcca acaaggacac cggcaactgg gagatcgagt tcttcaacag cgaccgctac
     5461 aacgccgcgc gcaatcgccg cgcgcccgac ggctacggcc ccgacacacc gattgcatga
     5521 acgagttcgt ctccgtccac accagcgatg aacagccggg catcgcgacg ctgctgctgt
     5581 cgcgaccgcc gaccaatgcg ctgacccgcc aggtgtatcg cgaaatcgtt tctgccgcag
     5641 cagatctcgg tcagcacgac gacatttccg cggtcatcgt gttcggcggt cacgagatct
     5701 tctccgccgg agacgatatg cccgagctca gcacgctgaa cgccgacgaa gccgacgtcg
     5761 ccgcgcgggt gtgccgggag gccgtcgacg cgttggccgc catcccgaag ccgaccgtcg
     5821 cggccatcac cggctacacg ctcggcggcg ggttgaacgt ggcgctggcc gcggactggc
     5881 gggtggccgg cgacaacgtg aagttcggcg cgacggagat cctggctggc cgggtgccac
     5941 agggcggcac ggaccgcctc gcacggacga ccggcgagag caaggccaag gacctggtgt
     6001 tcagcgggcg gttcatcgac gccaaggagg cgctggcgct gggcctgatc gacgagatgg
     6061 tggcacccga cggcgtgtac gacgccgcgc tggcgtgggc gcggcggttc ctcgactatc
     6121 caccgcaggc actggctgcc gccaaggcga ccttcgccgt ttaggctgcc ctgttatgag
     6181 ctttgaggaa accgacactc ctcctgatgt cgccgaccag gcggctccag tccccaaccc
     6241 gcacgccacg gcggaagagg tagaggcggc ccgccacgac tccaagctgg cccaggtgct
     6301 ctatcacgac tgggaagccg agagctacga cgagaagtgg tcgatcagct acgacaagcg
     6361 gtgcgtggac tatgcccgca acctgttcga cgcgaccgtc cccgacgaag agctgcgcga
     6421 actgccgtac ggccgggcgc tggagctggg ctgcggcagt ggattcttcc tgctcaacct
     6481 gatccaggcc ggcgtcgcca ggcgcggctc ggtgaccgat ctgtcgccgg gcatggtcaa
     6541 ggtggccacc cgcaacggcg agaacctcgg cctcgacgtc gacggccggg tcgccgacgc
     6601 cgagggcatc ccgtatgacg acgacacctt cgatctcgtc gtcgggcacg ccgtgctgca
     6661 ccacatcccc gacgtcgagc tgtcgctgcg cgaggtggtg cgggtgctca agccgggcgg
     6721 ccgattcgtc ttcgccggtg agccgacgaa tgcaggtgaa aactatgccc gcccgttgtc
     6781 gacgctgacg tggcgggcgg tgacgaacgt gacgaagctg ccggggctgg gtggctggcg
     6841 acggccgcag gcggagctcg acgagtcctc gcgcgcggca gcgctggagg cgatcgtcga
     6901 cttgcacacg ttcgcccccg gcgatttgga gcggatggcc cgcagcgcgg gtgccgtcga
     6961 ggtgtcgacc gccaccaccg agttcaccgc ggcgatgctc ggctggccgc tgcggaccct
     7021 ggaagccgcc gtgccacccg gcaagttggg ctggggctac gccaaattcg ccttccacag
     7081 ctggatcacg ctgagctggg ttgaccacaa cctgtggggc cgtgtggtgc cgaagagctg
     7141 gtactacaac gtgatggtga ccggggttaa gccgtcgtag ccttcggtct cggcgacgtt
     7201 gcctacctgc gatccgacgc gggcgcgcgg gatttgcgcg aggtcaacga ctttgggctg
     7261 ac
//
LOCUS       DASRJA010000354        11536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_751522,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000354 DASRJA010000000
VERSION     DASRJA010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11536
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751522"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /gene="ahcY"
                     /locus_tag="VFC01_15690"
     CDS             <1..1391
                     /gene="ahcY"
                     /locus_tag="VFC01_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZN81108.1"
                     /translation="FGRKEIRLAEHEMPGLMALRREYADVAPLKGARISGSLHMTVQT
                     AVLIETLVSLGAEVRWASCNIFSTQDHAAAAVVVGPHGTPEEPKGTPVFAWKGETLEE
                     YWWAAEQMLTWPGEPANMILDDGGDATMLVLRGAQYEKAGVVPPAEDDDSTEWKVFLG
                     LVRERFETEKDKWTKIAESVQGVTEETTTGVLRLYQFEAAGELPFPAINVNDSVTKSK
                     FDNKYGTRHSLIDGINRGTDVLIGGKKVLICGYGDVGKGCAESLAGQGARVAVTEIDP
                     INALQALMDGFDVVTVEQGISVADIVITATGNKDIITLAHMRSMKDQAILGNIGHFDD
                     EIQIAALERSGAKRLNIKPQVDQWIFDDGKSIIVLSEGRLLNLGNATGHPSFVMSNSF
                     SNQVIAQIELWTKNDEYDNAVYRLAKHLDEKVARIHVEALGGTLTKLTKEQAEYINVD
                     VEGPYKPEHYRY"
     gene            1543..3129
                     /locus_tag="VFC01_15695"
     CDS             1543..3129
                     /locus_tag="VFC01_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HZN81109.1"
                     /translation="MTTPLSWDLPYAWPRKPVLAGNVVCTTQPLAAQAGLAMLAEGGN
                     AVDAIIATAITITLVEPVSNGLGADAFAIVWDGEKLHGLNASGRSPAAWTPEYFDHAD
                     VPQRGWNSVTVPGAVSAWVELHAKFGKLPFERLFERAIRYGRDGFLVSPTIAGQWAAQ
                     VAELKSQPGFADAFMPGGRAPLPGERFRFPEHAATLEKIAQTNGEAFYRGELAEAIAA
                     DAAANGAGLLASDLAAHHPDWVKPISHDYRGYTVNEIPPNGQGVVTLMALGILEHFEI
                     ASLPVDSAESVHLQIEAVKLAFADALAYVADADHMSVRPDGLLDSGYLQRRAAMIDRD
                     KATAFTAGSPPRGGTVYLTAADSAGMMVSMIQSNNVGFGSGVVVPGTGISLHNRGAAF
                     VAAPDHPNRVGPRKRPYHTIIPGFVSKDGAPVMTMGVMGGPMQPQGQVQVMVRIADYL
                     QNPQAACDGPRFRWLSGLQVSLEDGFPSETVEGLRRRGHDLLAVDDYNAFGACQAIWR
                     MEDGYLGVSDPRRDGQAAAF"
     gene            3226..4539
                     /locus_tag="VFC01_15700"
     CDS             3226..4539
                     /locus_tag="VFC01_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HZN81110.1"
                     /translation="MGRGLVAFVVVVLIVVATGFAPAARADPSNDGHDPYYNEDEYSQ
                     FYTPPDPLPPGQPGDLIRTEPSRLVLEPSGELGAIMATGTRIMYRSTDARGNPMAVTG
                     TYFEPYNDWPGQGPRPLIVYGPGTQGQGDQCAPSRQFNQGIHWSPWLDLAFNYEELFV
                     ATMVARGFAIVMTDYQGLGTPGLHTYVNRIAEGNAMLDAGRAAMRLSGTSLDPHGPVA
                     FWGYSQGGVAAASAAELASSYAPDLHVVGTYAGAPPADLKELFPYADGSALVGVVGYA
                     LNSVITTYPEFEDAIRGTLTPRGADLLNKVQDQCIAETITKFMFRHLQPYFTSGIYQL
                     VNQEPFNTLFDMQRIGRYKPNAPVMILSNRYDPLVPWTAANQLGRDWCAQGADVQFWT
                     NEEPPFMNKLVVNHALPMLVDGERAMQWIADRFNGLPTTPNCGEF"
     gene            4637..5218
                     /locus_tag="VFC01_15705"
     CDS             4637..5218
                     /locus_tag="VFC01_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN81111.1"
                     /translation="MTKGTGDSAATVMAIDDPSVRFVRDVLPLVDQLYRAARRYTRSA
                     GDAEDLVQETMVKAYAGFHNFNAGTNIRAWLFRIMTNTWISSYRTAQRRVDEVLAADV
                     TDVQPNAAGGQSAELAALEAMGDKEVRDALQALPEGQRLVVYYADVEGFRYKEIAEIL
                     DVPLGTVMSRLHRGRANLRALLVDVAAARGYAA"
     gene            complement(5225..5584)
                     /locus_tag="VFC01_15710"
     CDS             complement(5225..5584)
                     /locus_tag="VFC01_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZN81112.1"
                     /translation="MMLSRLVTAVCAGAGIGAALFVASPLAHANQDPGANPPNCSAAD
                     LEGVRSGVDASTSAYLFTHPDLNDFMTTLKAMSREQVAAKVKGYMASHPDEQADMANI
                     RQPLTDIKDRCGAPPSP"
     gene            5767..6396
                     /locus_tag="VFC01_15715"
     CDS             5767..6396
                     /locus_tag="VFC01_15715"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZN81113.1"
                     /translation="MLIVIEGVDGAGKRTLTTGLRAAFEAAHKSVASLAFPRYGHSVE
                     ADLAAEALHGGHGDLAQSVYAMAVLFALDRAGARDEIAHLTDAYDVVILDRYVASNAA
                     YSAARLHQGADGEVVDWVRTLEYDRLHLPKPDAQVLLDVPTELAAERAEHRATQEADR
                     AKDAYERDDGLQRRTGEVYSGLAAAQWCGRWLVAGPDVNAPALAADLGQ"
     gene            6445..7131
                     /gene="mtrA"
                     /locus_tag="VFC01_15720"
     CDS             6445..7131
                     /gene="mtrA"
                     /locus_tag="VFC01_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MtrA"
                     /protein_id="HZN81114.1"
                     /translation="MDSMRQRILVVDDDPSLAEMLTIVLRGEGFDTAVIGDGTQALTA
                     VRELRPDLVLLDLMLPGMNGIDVCRVLRADSGVPIVMLTAKTDTVDVVLGLESGADDY
                     VMKPFKPKELVARVRARLRRNEDEPAEMLSIGDVDIDVPAHKVTRQGEQISLTPLEFD
                     LLVALARKPRQVFTRDVLLEQVWGYRHPADTRLVNVHVQRLRAKVEKDPENPQVVLTV
                     RGVGYKAGPP"
     gene            7128..8780
                     /gene="mtrB"
                     /locus_tag="VFC01_15725"
     CDS             7128..8780
                     /gene="mtrB"
                     /locus_tag="VFC01_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HZN81115.1"
                     /translation="MIFSSRRRIHRRSAPLLRGFGALSRAMGLAWRRSLQLRVVVLTL
                     GLSLAVILVLGFVLTSQITDRILEVKVRAATEEIERARNTVSGIVGGEETRSLDSSLQ
                     LARNTLIDRKADTGAGLAGAFDAVLVVPGDGPRAATAAGPVTQIPNALRDFVKAGQVS
                     YQYATVHTDGFSGPALIIGSPTSSPVTNLELYLIFPLNNEESTIALVRGTMLTGGVVL
                     LGLLAAIALLVARQIVLPVRSASRIAERFAEGHLTERMPVRGEDDMARLAVSFNDMAE
                     SLHRQITQLEEFGNLQRRFTSDVSHELRTPLTTVRMAADLIYDHSEDLDPALRRSTEL
                     MVNELDRFETLLNDLLEISRHDAGVAELSVESVDLRSTVQSALDNVGHLAADADSELI
                     VDMPADEVIAEVDPRRVERILRNLIANAIDHAEHKPVRIRMAADEDTVAVTVRDYGVG
                     LRPGEEKLVFSRFWRSDPSRVRRSGGTGLGLAISIEDARLHQGRLEAWGKPGKGACFR
                     LTLPLVRGHKVTTSPLPMKPIGRERPPRRSRDRDREPAQETV"
     gene            8798..10534
                     /gene="lpqB"
                     /locus_tag="VFC01_15730"
     CDS             8798..10534
                     /gene="lpqB"
                     /locus_tag="VFC01_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system accessory lipoprotein LpqB"
                     /protein_id="HZN81116.1"
                     /translation="MWLLVFVLAGCAGVPSSSAPQAIGTVDRPAPPSLPKPVPGMDPD
                     VLLREFLKATADPANRHLAARQFLTESASRAWDDAGSALLIDNVVFVETRGPDRVSVS
                     MRADILGSLSDVGVFETGEGALPDPGPIELVKTPGGWRIDRLPNGVFLDWQQFQSTYK
                     RSTLYFADPTGKTVVPDPRYVAVSDSDQLATELISKLIAGPRPEMDKTVRNLLGPPLK
                     LRGPVTRADGGKTGVGRGYGGARIDLENLSTTDPHSRQLLAAQIIWTLARAGINGPYV
                     INADGAALDDRFADGWNTSDVAATDPGAADGAAAGLHALVGGTLMSLDGQRATRVAGQ
                     FGQMPGQTAAAVSRTGQEVASIVTLRPGALDMASSLWVGPLNGNATQAMEARTLSRPS
                     WSLDDAIWVVVDGINVVRAIQDASGQPARIPVDSSVVSMRFPGAIAELQLSRDGTRAA
                     MIIEGQVVLAGVEQTAGGGYALTYPRRLGFGLGNTAVSLSWRTGDDIVVSRTDPQHPV
                     SYVNLDGVNSDGPSRNLLMPVTTVAANPSTVYVADERGVLQLSGSAADNDPAWAEVRA
                     LMMPDAVPVLPG"
     gene            10637..11302
                     /locus_tag="VFC01_15735"
     CDS             10637..11302
                     /locus_tag="VFC01_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81117.1"
                     /translation="MKIRGPLVTLGAVAVLGIGILLVNMSKEESPPPGKSAAESTTAV
                     ATTTTAAPAPAPPSTPPPPAFPAKADYVGKIPTANGTITLEITVDGDKAIAYACDGNT
                     VESWLRGSAVNGAVSLANKDKTSRLEGRLEGNAVVGTLWIGEKQWDFNAPAAQPPAGL
                     YVYENAGVRNSWIIDPSGGVTGVQRREDGSTAPAPSLTTDGIADIDGIEVKAIRVEGD
                     SNV"
     gene            11295..>11536
                     /locus_tag="VFC01_15740"
     CDS             11295..>11536
                     /locus_tag="VFC01_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6529 family protein"
                     /protein_id="HZN81118.1"
                     /translation="MSDAEAPTTAVPARSSSGIAVLVAVAIGALVAVGLGVFGKLHEP
                     QFFSISVAGFSSGLAVKSWLATLAVVLALFQLASAFA"
BASE COUNT         1984 a         3749 c         3890 g         1913 t
ORIGIN      
        1 agttcggtcg caaggagatc cggttggccg agcacgagat gcctggattg atggcgctgc
       61 ggcgcgagta tgccgacgtg gcaccgctca agggcgcgcg catctccggg tcgctgcaca
      121 tgacggtgca gaccgccgtg ctgatcgaga cgctggtttc cctcggcgct gaagtgcgat
      181 gggcgtcgtg caacatcttc tccacccagg accacgccgc cgcggcggtc gtcgtcggcc
      241 cgcacggcac gccggaggag cccaagggca ccccggtgtt cgcgtggaag ggcgagaccc
      301 tcgaggagta ctggtgggcc gccgagcaga tgctgacctg gccgggcgag ccggccaaca
      361 tgatcctcga cgacggtggt gatgccacca tgctggtgct gcgcggtgcg cagtacgaga
      421 aggccggcgt cgtcccgccc gccgaggacg acgactcgac cgagtggaag gtcttcctcg
      481 gtctggtgcg cgagcgtttc gaaaccgaga aggacaagtg gaccaagatc gcagagtcgg
      541 tccaaggtgt caccgaggag accacaaccg gcgtgctgcg gctgtatcag ttcgaggccg
      601 ccggtgagct tccgttcccg gcgatcaacg tcaacgactc ggtgaccaag agcaagttcg
      661 acaacaagta cggcacccgg cactcgctga tcgacggcat caaccgcggc actgatgtgc
      721 tgatcggcgg caagaaggtg ctgatctgtg gctacggcga tgtcggcaag ggttgcgcgg
      781 agtcgttggc gggccagggc gcgcgggtgg cggtgaccga gatcgacccg atcaacgcgc
      841 tgcaggcgct gatggacggt ttcgacgtgg tgacggtcga gcagggcatc agcgtcgccg
      901 acatcgtgat cacggcgacg ggcaacaagg acatcatcac gttggcgcac atgcggtcga
      961 tgaaggacca ggccatcctg ggcaacatcg gtcacttcga cgacgagatt cagatcgcgg
     1021 cgcttgagcg gtcgggcgcc aagcgtctga acatcaagcc gcaggtggac cagtggatct
     1081 ttgatgacgg caagtcgatc atcgtgctgt cggagggccg gctgctgaac ctgggcaatg
     1141 cgaccggaca cccgtcgttc gtgatgagca acagcttctc caaccaggtg atcgcgcaga
     1201 tcgagctgtg gaccaagaac gacgagtacg acaacgcggt gtaccggttg gccaagcacc
     1261 tcgatgagaa ggtcgcgcgc attcacgtcg aggcgctcgg cggaacgctg accaagctca
     1321 ccaaggagca agcggagtac atcaacgtcg acgtcgaggg cccgtacaag cccgagcact
     1381 accgctactg agcttgggcc cccgcgcatg ggcccacgcg aaagctgggg actggggacg
     1441 cgcatcctcg agactgcggg cagatcgcga tcgacgccaa aatcgtgatc tgaccgcagt
     1501 ttcgtcgtcg tcgcagtcac atccgggccg taggattcgc ccgtgactac cccgttgtcg
     1561 tgggacttgc catatgcgtg gcccagaaag cccgtgctgg caggcaatgt cgtgtgcacg
     1621 acgcaaccgc ttgccgcgca agccggcctc gcgatgcttg ccgaaggcgg caacgcggtt
     1681 gatgcgatca tcgcgacggc gatcaccatc acactggtcg aaccggtgtc caacggcttg
     1741 ggtgctgacg catttgcgat cgtgtgggac ggcgaaaaac ttcacgggct caatgcatcc
     1801 ggacgttcgc ccgcggcgtg gacacccgaa tactttgacc acgcggacgt cccgcagcgc
     1861 ggctggaatt cagtgactgt cccgggtgcg gtctcggcat gggtcgagct gcacgccaag
     1921 ttcggcaagc tgccgttcga gcgactcttc gagcgggcga tccgctacgg ccgcgacggc
     1981 ttcctggttt ctccgacgat cgccggtcag tgggcggcac aggtcgcgga gctgaaatca
     2041 cagcccggct tcgccgacgc cttcatgccc ggcgggcgag ccccactgcc cggtgaacgg
     2101 tttcggtttc cggagcacgc ggcgacgttg gagaagatcg cgcagaccaa cggcgaagcg
     2161 ttctatcgcg gcgaacttgc ggaggcgatc gctgcggacg ctgcggccaa tggtgctggc
     2221 ctgctcgcct ctgacctcgc tgcgcatcat cccgactggg tgaagccgat cagccacgac
     2281 taccgcggct acaccgtgaa cgagatccca cccaacgggc aaggcgtcgt cacgctgatg
     2341 gcactgggca tcctcgagca cttcgaaatc gcgtcgctgc ctgtcgattc ggccgagagc
     2401 gtgcatctgc agatcgaggc cgtgaagctg gcgttcgccg acgcgctggc gtatgtcgcc
     2461 gatgctgacc acatgtcggt tcgtccggac ggtctgctcg acagcgggta tctccagcgg
     2521 cgcgcggcga tgatcgaccg ggacaaggcc actgcgttca cggcgggcag cccgccacgc
     2581 ggcggcacgg tgtacctgac cgctgccgac tccgccggga tgatggtgtc gatgatccag
     2641 tccaacaatg tgggcttcgg gtcgggagtc gtggtgccgg gcaccggcat ctcgctgcac
     2701 aaccgcggcg ccgccttcgt cgctgcaccc gaccacccga accgggtcgg tccgcgaaag
     2761 cgcccgtacc acaccatcat tccgggattc gtcagcaagg acggcgctcc ggtgatgacg
     2821 atgggcgtga tgggtgggcc gatgcagccg caggggcagg tgcaggtgat ggtccggatc
     2881 gccgactacc tgcagaatcc gcaggccgcg tgtgacgggc cgcgattccg ctggttatcc
     2941 ggattgcagg tgagcctgga agacggattt ccgtcggaga ccgtcgaggg gctgcgccgc
     3001 cgtggccacg atctgctggc cgtcgacgac tacaacgcct tcggggcatg ccaggcgatc
     3061 tggcggatgg aggacggcta tctcggcgtg agcgacccac gccgagacgg gcaggcggcg
     3121 gccttctaac cgcgctcacc tcacaagctt gctggccggc gtgtcgaact tacccaatag
     3181 taagaaactg cccgtcgcgc atactggacc tgttacggtc ccccggtggg tcggggactc
     3241 gtggcgtttg tcgttgtcgt acttatcgtt gtagcaaccg gtttcgcgcc cgcggcgcgc
     3301 gcagatccca gcaacgatgg ccacgatccg tactacaacg aagacgaata ctcccagttc
     3361 tacaccccgc ctgatccgct gccgccgggt cagcccggcg atctgatccg taccgaaccg
     3421 tcgcggttgg tgctcgagcc gtcgggcgag ctgggcgcga tcatggccac cggcacgcga
     3481 atcatgtacc gcagcaccga cgctcgtgga aacccaatgg cggtgacggg cacctacttc
     3541 gaaccgtaca acgactggcc cgggcagggg ccgcggccgc tgatcgtgta cggccccggc
     3601 acccaaggcc agggcgacca atgtgcgccg tcgcgtcagt tcaatcaggg tatccactgg
     3661 tcgccgtggc tggacttggc gttcaactac gaggagctgt tcgtcgcgac gatggtcgcg
     3721 cgcgggttcg cgatcgtgat gaccgactac caggggctcg ggacgccggg actgcacacg
     3781 tatgtcaacc ggatcgcgga gggcaacgcg atgctcgacg cgggccgggc ggcgatgcgg
     3841 ttgtccggca cctcgttgga tccccatggg cccgtggcat tttggggtta ttcgcagggt
     3901 ggcgttgctg cggcctcggc ggccgaactg gcgtcctcct acgcgccgga ccttcacgtc
     3961 gtcggcacgt acgcgggtgc gccgccggcc gatctgaagg aattgtttcc ctacgccgac
     4021 ggcagcgcgc tggtgggagt cgtcggctat gcgctcaact ccgtcatcac cacctacccg
     4081 gaattcgagg atgcgattcg cgggacgctc acgccgcgcg gcgcggatct gctgaacaaa
     4141 gtccaagacc agtgcattgc ggagacgatt acgaagttca tgtttcggca cctgcagccc
     4201 tacttcacct cgggcatcta ccagctggtc aaccaggaac ccttcaacac cctgtttgat
     4261 atgcagcgca tcggccgcta caaacccaac gcgccggtga tgatcctgag caaccggtat
     4321 gacccgttgg tgccgtggac ggcggccaac cagttggggc gcgactggtg cgcacagggc
     4381 gccgacgttc agttttggac caacgaggag ccgccgttca tgaacaagct ggtcgtcaac
     4441 cacgcgctgc cgatgctggt ggacggcgag cgggccatgc aatggatcgc cgaccggttc
     4501 aacggattgc cgacgacacc gaactgcggc gagttctgag cagaggcgag cctgatctcc
     4561 gccagaggac gagggcattt gcgtattcgg caaaatcgct tgacctcaag tccgcttgag
     4621 gtgccacgct gctggcatga caaaggggac aggggattcc gccgcgacgg tgatggctat
     4681 cgacgacccg tcggtacggt tcgtccgaga cgtgttgccg ctggtcgacc agctgtaccg
     4741 cgccgcgcgc aggtacaccc gcagcgccgg cgatgccgag gacttggtcc aggaaacgat
     4801 ggtcaaggcc tatgcgggtt ttcacaactt caatgccgga accaatatcc gcgcatggct
     4861 gttccgcatc atgaccaaca cgtggatcag ctcgtaccgc acggcgcagc gacgcgtcga
     4921 tgaggtgctg gccgcagatg tcaccgacgt gcagccgaac gcggccggcg gccaatcagc
     4981 cgaacttgct gcgttggaag cgatgggcga taaggaggtg cgcgacgcgc tgcaggccct
     5041 gcccgaaggc cagcgcctgg tggtctacta cgccgatgta gaaggcttcc ggtacaagga
     5101 gattgctgag atcttggacg ttccgctcgg cacggtgatg tcacggctgc accgcggacg
     5161 ggccaatttg cgcgcgctgc tcgttgacgt cgccgcggcg cgcggttacg cggcgtagga
     5221 cgggctatgg actcggcggt gctccgcagc gatccttgat gtcggtgagc ggctgccgaa
     5281 tgttggccat gtcggcttgc tcgtcagggt gagaggccat gtagcccttc actttcgcgg
     5341 ctacctgttc acgggacatc gccttgaggg tcgtcatgaa gtcgttcagg tcgggatgcg
     5401 tgaacaagta ggccgacgtc gacgcatcga ctcctgacct gactccctcg aggtcggcgg
     5461 cgctgcagtt cgggggattg gcgccgggat cctggttggc atgggcgagc gggctggcga
     5521 cgaacagcgc cgcaccgatc cccgcgccgg cacacacggc ggtgacgagg cgagacaaca
     5581 tcatggctca cagtctgcgc acaacccacg ctcatgtgcg cgcgtcgcaa gcagttccac
     5641 agcagattcg gcttgtgccc acagcaagcc ggcgttgtgc tacatccgac gaacgttccg
     5701 taccgctcga aaaatcgccg aatagtcgag cggtaaggaa cgttcgcagc cggataggct
     5761 cgccgcgtgc tcatcgttat cgagggtgtc gacggcgcag gcaaacgcac gctcaccact
     5821 ggcctgcgcg ctgcgttcga agccgcgcac aagtccgtcg ccagcctcgc cttcccgcgg
     5881 tacggccatt cggttgaagc cgacctcgcc gcggaggcct tacacggcgg gcacggcgac
     5941 ctcgcgcagt cggtgtacgc gatggccgtg ctgtttgccc tcgaccgcgc gggcgcccgc
     6001 gacgagatcg cccatctgac cgacgcctac gacgtcgtga tcctcgatcg gtacgtggcg
     6061 tccaacgccg cctacagcgc cgcgcggctg caccagggcg ccgacggcga ggtcgtcgat
     6121 tgggtgcgca ccctcgaata cgaccgattg cacctgccca agccggacgc ccaagtgctg
     6181 ctcgacgtgc cgaccgaact cgccgccgag cgcgcggagc accgcgccac ccaggaagcc
     6241 gaccgcgcca aggacgccta cgagcgcgac gacgggctgc agcggcgcac cggcgaggtc
     6301 tactccgggc tcgcagcggc gcagtggtgc ggacgctggt tggttgccgg gcccgatgtg
     6361 aacgcccctg cgctggccgc agacctgggt caatagccga ctcgcgggtg tgctaccggc
     6421 gttttatcgc aatttggtga caccatggac tccatgaggc aaaggattct ggttgtcgac
     6481 gacgaccctt cgctggccga gatgctcacc atcgtcctgc gcggggaagg tttcgacacc
     6541 gcggtcatcg gcgatggcac gcaggcgctc acggcggtcc gcgaattgcg gcccgatttg
     6601 gtgttgctgg acctgatgct gccaggcatg aacggcatcg acgtatgccg cgttctgcgc
     6661 gccgattccg gtgtgccgat cgtcatgctg acggccaaga ccgacaccgt cgacgtggtg
     6721 ctcggcctgg agtcgggcgc cgacgactac gtcatgaagc ccttcaagcc caaggagctc
     6781 gtcgcccgtg tgcgcgcccg gttgcgccgc aacgaagacg agcccgcgga gatgctgtcg
     6841 atcggcgacg tcgacatcga cgtgccggcc cacaaggtca cccggcaagg tgaacagatt
     6901 tccctgacac cgctggagtt cgacctgttg gtggcgttgg cgcgcaaacc acggcaggtg
     6961 tttactcgtg atgtgctgct cgaacaggtg tggggatacc ggcaccccgc cgacacccgt
     7021 ttggtgaacg tgcatgtcca acgtctgcgg gccaaggttg agaaagaccc ggagaacccg
     7081 caggtggtac tgaccgttcg aggagtggga tacaaggccg gacccccgtg atcttcagct
     7141 cgaggcggcg catccatcgg cgttcggcac cactactacg cggattcggt gcgctgagtc
     7201 gggcaatggg tttggcgtgg cgccgctctc tgcagttgcg ggtggtggtg ttgacgctgg
     7261 ggctgtcgct cgccgtcatt ttggtgctcg gcttcgtgct gaccagtcag atcaccgacc
     7321 gcatcctcga ggtcaaagtc cgtgccgcca ccgaggagat cgagcgcgcc cgcaacacgg
     7381 tcagcggcat cgtcggcggc gaggaaaccc gctcactcga cagcagcctg caactggcgc
     7441 gcaacaccct gatcgaccgc aaggccgaca ccggcgccgg gctcgcgggc gcgttcgacg
     7501 cggtgctggt ggttcccggt gacggccccc gggcggcgac cgcggcaggt ccggtgactc
     7561 agatccccaa cgcgttgcgt gacttcgtca aggccggcca ggtcagctat cagtacgcga
     7621 ccgtgcacac cgacgggttc tccggcccgg cgttgatcat cggcagtccc acgtcatcgc
     7681 ccgtgacgaa tctcgagctg tacctgatct ttccgctcaa caacgaggaa tccaccatcg
     7741 cgctggtgcg cggcaccatg ctgaccggcg gcgtggtgct gctcggtctg ctggccgcga
     7801 tcgcgctact ggtggcccgc cagatcgtgc tgccggtgcg gtcggcatcg cggatcgccg
     7861 agcgcttcgc cgagggccac ctcaccgagc ggatgccggt gcgcggcgag gacgacatgg
     7921 cgcggctggc ggtgtcgttc aacgacatgg ccgaaagcct gcaccgccag atcacccagc
     7981 tcgaggagtt cggcaacctg cagcgccggt tcacctccga cgtcagccat gagttgcgca
     8041 caccgctgac aaccgtgcgg atggccgccg acctgatcta cgaccacagc gaggacctcg
     8101 accctgctct gcgacgttcg accgagttga tggtcaacga gctcgaccgg ttcgaaacgc
     8161 tgctcaacga cctgttggaa atctcgcgcc acgacgccgg tgtcgcggag ctgtcggtcg
     8221 agtcggtcga tctgcgctcg acggtgcaga gcgcgctgga caacgtcggc catctggcgg
     8281 ccgacgcgga cagcgagctg atcgtcgaca tgccggccga cgaggtgatc gccgaagtcg
     8341 atccgcgccg ggtggaacgg atcctgcgca acctgatcgc gaacgcgatc gatcacgccg
     8401 agcacaagcc ggtgcggatc cggatggccg ccgacgagga caccgtcgcg gtcaccgttc
     8461 gcgactacgg tgtcgggcta cggccgggcg aggagaagct ggtattcagc cggttctggc
     8521 gctccgaccc gtcgcgggtg cggcgctccg gcggcaccgg cctcggcttg gcgatcagca
     8581 tcgaggacgc tcggctgcac cagggtcggc tggaggcgtg gggcaaaccc ggcaagggcg
     8641 cgtgcttccg gctcacgctt ccgctggtgc gcggacacaa ggtgacaacg agtccgctgc
     8701 cgatgaaacc cattgggcgc gaacggccgc cgcgccggtc acgtgaccgc gaccgggaac
     8761 ccgcccagga gaccgtgtga aacgcgtgct ggcagccatg tggttgctgg tgttcgttct
     8821 cgcgggctgc gccggcgtcc cgagttcttc tgcgccgcag gccatcggca ccgtcgaccg
     8881 tcccgcgccg ccgagcctgc ccaagccggt acccggcatg gaccccgatg tgctgctgcg
     8941 ggaattcctc aaggccacag ccgatcccgc caaccggcat ctggccgcgc ggcagttcct
     9001 caccgaatcc gcctcgcggg catgggacga cgccggtagt gccctactga tcgacaacgt
     9061 cgtgttcgtc gaaacacgcg gccccgaccg ggtttccgtc agcatgcgcg ccgacatcct
     9121 cggctcgctc tcggatgtcg gagtgttcga gaccggcgag ggtgcgctgc cggatcccgg
     9181 tccgatcgaa ctggtcaaga cgcccggcgg gtggcgcatc gaccggctgc ccaacggcgt
     9241 tttccttgac tggcaacagt ttcaatccac ctacaagcgc agcacgctgt atttcgccga
     9301 tcccaccggc aagaccgtgg tgcccgaccc gcggtatgtc gcggtgtccg acagtgacca
     9361 gctggccacc gaactgatca gcaagctgat cgcaggccca cggcccgaga tggacaagac
     9421 ggtgcgcaat ttgctcggtc cgccgctaaa gctgcgcggc ccggtaacgc gcgccgacgg
     9481 cggcaagacc ggcgtgggcc gcggctacgg cggtgcccgg atcgacctgg agaacctgtc
     9541 gacgaccgac ccgcacagcc ggcaactgct tgcggcacag atcatttgga cgctggcgcg
     9601 ggccgggata aacgggccgt acgtgatcaa cgccgacggg gcggcgctcg acgatcgctt
     9661 cgccgatggc tggaacacct ccgacgtcgc ggccaccgac cccggtgcgg ccgacggtgc
     9721 cgccgcggga ttgcacgcgt tggtgggcgg cacgctgatg tcactggacg gccagcgcgc
     9781 gacaagggtg gccggccagt tcggccagat gcccggccag accgcagctg ccgtgtcacg
     9841 caccggccag gaagtggcgt cgatcgtgac gctgcggccc ggtgcgctcg acatggcgtc
     9901 gtcgctgtgg gtcgggccgc tgaacggcaa tgcgacacag gcgatggaag ctcgcacgct
     9961 gtcgcggccc agctggtccc ttgatgacgc catctgggtc gtggtggacg gcatcaacgt
    10021 cgtgcgcgcg attcaggacg cgtcggggca gcctgcccgc atccctgtcg actccagcgt
    10081 ggtgtcgatg cgctttcccg gcgcgatcgc cgaactccag ctctcccgcg acggaacgcg
    10141 cgccgcgatg atcattgaag ggcaagtggt tctcgccggt gtcgaacaga cggccggcgg
    10201 tggttatgca ctcacctatc cgcggcggct gggcttcggc ctcggcaaca cggcggtgtc
    10261 gctgtcctgg cgcaccggcg acgacatcgt cgtcagccgc accgacccgc aacacccggt
    10321 gtcctacgtc aacctcgacg gggtgaactc cgacggcccg agccggaacc tgctaatgcc
    10381 ggtcaccacc gtcgccgcca acccgtccac cgtgtacgtc gccgacgagc gtggtgtgct
    10441 gcaactgtcc ggctcggccg ccgacaacga cccggcgtgg gccgaagtgc gggcgttgat
    10501 gatgccggac gcggtgcccg tattgccggg ctaattcccg aactttttga gaaatcgttc
    10561 tgaaccatct cccggcatcg gccgttgtgc agacaaaaca tcaccagagg tgtttgctca
    10621 acagccagga gggttgatga agatccgcgg tccgttggtc acactcggcg ccgttgcggt
    10681 gctcgggatc ggcatattgc tcgtgaacat gtccaaggag gagtcgccgc cgccgggcaa
    10741 gtctgccgcc gagtccacca ccgccgtcgc caccacgact accgccgcgc ccgcgccggc
    10801 accaccgagc acgccgccac cgccggcgtt tccggccaag gccgattacg tcggcaagat
    10861 cccgaccgcc aacggcacga tcacgttgga gatcaccgtc gacggcgaca aggccatcgc
    10921 ctacgcgtgc gacggcaaca ccgtcgagtc gtggctgcgg ggtagcgccg tcaacggcgc
    10981 ggtcagcctg gccaacaagg acaagacgag ccggctcgag ggccgcctgg aaggcaatgc
    11041 cgtcgtcggc acgttgtgga tcggtgaaaa gcagtgggac ttcaacgctc ccgcagcgca
    11101 gccgcccgcc gggctgtatg tctacgagaa cgccggtgtt cgcaacagct ggatcatcga
    11161 cccgagtggg ggcgtgaccg gcgtgcagcg acgggaggac gggtcgaccg cacccgcgcc
    11221 cagcctgacg accgacggca tcgcggacat cgacggcatc gaggtcaagg cgatccgggt
    11281 tgagggggac agcaatgtct gatgccgagg ctccgaccac ggccgtgccc gcgaggtcgt
    11341 cgtcggggat agcggtgctg gtcgcggtcg ccatcggcgc gctggtcgcc gtcggactcg
    11401 gtgtgttcgg gaaactccat gagccgcagt tcttttcgat cagcgttgcc ggcttctcca
    11461 gcgggcttgc cgtgaagtct tggctcgcga cgctggctgt ggtgctggcg ctgttccagc
    11521 tggcgtccgc attcgc
//
LOCUS       DASRJA010000355        14926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1870233,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000355 DASRJA010000000
VERSION     DASRJA010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14926
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870233"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFC01_15745"
     CDS             complement(<1..354)
                     /locus_tag="VFC01_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HZN81119.1"
                     /translation="MSIREGVAPNRIITKLLGYVSLTKPRVIELLLVTTIPAMLLAHR
                     GTVDPLLIVNTMFGGLLAAAGANTLNCVADADIDKVMKRTSRRPLAQHTVPRSHALVF
                     GLALSVASFFWLWWTT"
     gene            523..2613
                     /gene="tkt"
                     /locus_tag="VFC01_15750"
     CDS             523..2613
                     /gene="tkt"
                     /locus_tag="VFC01_15750"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZN81120.1"
                     /translation="MTTLDEISTLTRPNHPDDWTELDSAAVDTVRVLAADAVQKVGNG
                     HPGTAMSLAPLAYTLFQRQMHHDPSDTHWLGRDRFVLSAGHSSLTLYIQLYLGGFGLE
                     LSDIESLRTWKSRTPGHPEFRHTPGVEITTGPLGQGLASAVGMAMASRYERGLFDPDA
                     APGTSPFDHYIYVIASDGDMEEGVTSEASSLAGTQQLGNLIVFYDSNQISIEDDTNIA
                     LTEDTPARYRAYGWHVQEVEGGENVVGIEEAIAEAKRVTDKPSFIAIRTIIGYPAPKL
                     MNTGKAHGAALGDEEVAAVKKILGFDPDKTFEVRDEVLEHTRRLVARGKEAHEKWQSE
                     FDAWAQREPDRKALLDRLTEHKLPDGWDADLTYWEPGSKALATRAAFGQVLNDVAPKL
                     PELWGGSADLAGSNNTTIKGVKSFGPPSISTKEFTADPYGRVLHFGVREHAMGSILSG
                     IVLHGPTRAFGGTFLQFSDYMRPAVRLASLMNIDTIYIWTHDSIGLGEDGPTHQPIEH
                     LAALRAIPNLSVVRPGDSNETAYAWRTILARGASSGPTGFILTRQGIPVLEGTDAEGV
                     ARGGYVLGGTPDEDPDVVLIGTGSELQLAVAAQKILADKGITATVVSMPCVEWFESQP
                     AEYRDSVLPPAVSARVAVEAAVAQSWYKLVGDTGEIVSIEHYGESADDKTLFREFGFT
                     PEAVVAAAERSLDN"
     gene            2632..3750
                     /gene="tal"
                     /locus_tag="VFC01_15755"
     CDS             2632..3750
                     /gene="tal"
                     /locus_tag="VFC01_15755"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HZN81121.1"
                     /translation="MTQNPNLAALSAAGVSVWLDDLSRNRLQTGNLQELIDTKSVVGV
                     TTNPTIFQGALSKGTAYDEQVKELADRGADVDATIRTVTTDDVRNACDVLAKAWEASN
                     GVDGRVSIEVDPRLAHETDKTILQAVELWKIVDRPNLLIKIPATLAGLPAITAVIAEG
                     ISVNVTLIFSVQRHQAVMDAYLSGLEKAKEAGHDVSKIHSVASFFVSRVDTEIDKRLE
                     KIGTDDALALRGQAGVANARLAYAAYEEVFVGGSRYEALKAEGARVQRPLWASTGVKN
                     PEYSDTLYVTELVAPNTVNTMPEKTIDAVADHGVIAGDTVTGKAAEAQATFDELDAVG
                     IDLPDVFKVLEDEGVEKFEKSWLELLEATQGQLDAAKK"
     gene            3747..5300
                     /gene="zwf"
                     /locus_tag="VFC01_15760"
     CDS             3747..5300
                     /gene="zwf"
                     /locus_tag="VFC01_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZN81122.1"
                     /translation="MTAVNDKSTSTSWRNPLRDKRDKRMPRIPGPCGVVIFGVTGDLA
                     TKKLMPAIYDLANRGLLPASFALVGFARRDWADEDFGKLVCDAVRKHARTPYRQEVWD
                     RLAEGIRFVQGTFDDDKAFERLAETLAKLDAERGTGGNHAFYLSIPPKAFQQVCEQLQ
                     KSGLARPQDGRWSRVVIEKPFGHDLQSAQELNGVVNSVFPEESVFRIDHYLGKETVQN
                     ILALRFANELYEPIWNNNYVDSVQITMAEDIGLGGRGGYYDGVGAARDVIQNHLLQLL
                     ALTAMEEPVNFSPHELQAEKIKVLSAIRLMQPLDDTTARGQYGAGWQGSEKVVGLLDE
                     EGFSKESTTETYAAITLEIDTRRWAGVPFFLRTGKRLGRRVTEIALIFKRAPHLPFDK
                     TMTEELGQNALVFRVQPDEGITTRFGSKVPGSAMEVRDVNMDFSYGSAFAEDSPEAYE
                     RLILDMLLGEPSLFPVNREVELSWEILDPVLDYWASHGKPDTYESGTWGPASADEMMH
                     RMGREWRRP"
     gene            5320..6231
                     /gene="opcA"
                     /locus_tag="VFC01_15765"
     CDS             5320..6231
                     /gene="opcA"
                     /locus_tag="VFC01_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HZN81123.1"
                     /translation="MIVDLPDTTTNDINKKITGLREEGGAITLSRVLTLVISLDSDDV
                     LEDSIEAANFASREHPCRVIVVVQGDRSASEPRLDAQLRVGADAGAGEVVALRLHGEL
                     ADHASSVVLPFLLPDTPVVAWWPVGGPAVPAQDPLGHLAIRRITNATACEDPLAAIKG
                     RLQGYTDGDTDLAWARITYWRALLASALDQPPFEPVTSAVVSGLRDEPSLDVLAGWLA
                     VRIDGPVQRAVGELKVELIRSSETITLKRPQTGVTATLTRTSRPEARIPLARREAKEC
                     LAEDLRRLDADEIYHEALQGIDKVQYT"
     gene            6311..7048
                     /gene="pgl"
                     /locus_tag="VFC01_15770"
     CDS             6311..7048
                     /gene="pgl"
                     /locus_tag="VFC01_15770"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZN81124.1"
                     /translation="MNSVIEKYADSAALVAAAGDRLVDAITDAIDKRGTAQIVLTGGG
                     TGVGLLKRVGERGEKIDWSKVHLYWGDDRFVPADDDERNDKQAADALLDHIDIPDANV
                     HPMSASDGEFGDDLDAAARDYEQVLAANADDGEPAPDFDVHLLGMGGEGHINSLFPET
                     PAVRETSRLVVGVEDSPKPPPRRITLTLPAVQRSREVWLVVSGAAKADAVAAAIGGAK
                     PVDVPAAGAVGRDATVWLLDEDAAAKL"
     gene            complement(7045..9237)
                     /gene="helR"
                     /locus_tag="VFC01_15775"
     CDS             complement(7045..9237)
                     /gene="helR"
                     /locus_tag="VFC01_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor ATPase HelR"
                     /protein_id="HZN81125.1"
                     /translation="MSTRSYDEELQGERGYVAGLYARLDAERARVRGRYGVALRSPVD
                     AGNGAPLVERDVEVRALAKQLNRLNVADHGLCFGRLDSLSGERLYIGRIGLFDEQNDY
                     EPLLLDWRAPAARAFYVATAASPENMRRRRQFHTRGRQVVDFTDEMFGRPGDGQRGDA
                     ALLAAVNAPRSDGMRDIVATIQAQQDEIIRLDHSGVLVIEGGPGTGKTVVALHRVAYL
                     LYTQRERIERHGVLVVGPNTAFLNHIGRVLPSLGETNVVFMTTGDLVPGLHITAEDTA
                     EAARLKGSLKILEVLAAAVADRQRLPEDPLSIKLSDVTVRIDAETAEWAIQEARASGQ
                     PHNEARAVFREIVTYVLTERAIARIGAGWLTRKDRDAWEHLRADLIDELADNDQFTAA
                     LDELWPILTPETLLAQLYESPERLRAAGADPALWRADGDAWTVSDVPLLDELVDLLGR
                     DKPADQSADRERLAEAEYAAGVLDLMVSREDLMDDEDHLLATDMIYAEDLADRFAERD
                     TRELAERAAADRDWTYRHAVVDEAQELSEMDWRVLMRRCPSRSFTVVGDLAQRRSMSG
                     ATSWDTMLEAYVPGRWVYRSLSVNYRTPAEIMTVAGALLAEFAPGVEPPESVRACGIQ
                     PWSQRVTDDELPTAIEEFVIDEADREGTSIVIGPPDVPGAVVPSETKGLEFDAVLVVE
                     PERILADGPRGAADLYVALTRATQRLGVLHRDPLPEVLTGLDARVPSY"
     gene            9786..11222
                     /locus_tag="VFC01_15780"
     CDS             9786..11222
                     /locus_tag="VFC01_15780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HZN81126.1"
                     /translation="MFEDVPDGELIDVMGEATRDESTAIAQRLAAVAQLYVRRSGELA
                     DHQWWCADACDAVAAEISAAQNISHARAVAQVQFACAVHDRLPQVAKVFATGVIDFRL
                     VRTLIARTENVDDAVMPELDAAIARHCQKWMKLSGPKVRDRVDMWVAKFDPAGVRVPP
                     TVEENRYVEVGQTSPGMAGLSGHVHATDAAVFDRRLDALADTVCENDPRTKAQRRADA
                     TGAVGRGEATLACQCGLADCPARAERDGAAAAAAAVIHVLAERATLDGASDQPGYLPG
                     FGVLPAESVREVAKTARLKPVVIPSDTPDPGYRPSAVTREFLVWRDLTCRWPGCDRPV
                     EKCDVDHTVPYPAGLTHPSDLKHYCRIHHLIKTFYGGPGGWTDQQMPDGTVILTAPTG
                     HSYTTEAHGARMFPALAQSTGELPIPPALDLPDTDRSVMMPRRKQTREQDRRDRINAE
                     RRERTELIAEEERQRQAWLAANYEPPPF"
     gene            11438..11809
                     /locus_tag="VFC01_15785"
     CDS             11438..11809
                     /locus_tag="VFC01_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZN81127.1"
                     /translation="MADKGGKPARTGSERIRKLAQAALNADVTVEQVDTILEGLSETL
                     VDLNNSTGNLDATLERFNETINQINELAPRLNAVVDRMEGIVSRVERIVGIGEAVVSP
                     LTATEQAVRGAVNAVRRSTGL"
     gene            11809..13104
                     /locus_tag="VFC01_15790"
     CDS             11809..13104
                     /locus_tag="VFC01_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome"
                     /protein_id="HZN81128.1"
                     /translation="MTRGSISPAPPVAEGTGLPWDVTVDDAVAAIAEARARHGDTFVA
                     HSGRDHYLFTFSPVGVESFYALPEEIASKGLADYLMLRRKLPDEIFDGRRTLPASLFR
                     RDDVTSYLVNLDQALEQTVAELGPEGTVDLFDLTRRLGHRMGLASWAGPGSTQADTFD
                     RLVAAFDILDGSDAFVHPDAMAAVAATDKRAERAALDDVVELVSIAVRRLDSGQTTDD
                     ALFGRIVEAWSSEPPDIRTRGIALDVALIHIASMSNLMAALGWAFVDLLEHPTHLRLV
                     AAGEEELARRCALESTRLAQRSIMSRTVLAPVDFDTGDACYRVPAGWTIATLLPLLNT
                     SAAPGLRDWDPDRWHRHRLADPTALPSPMLVTAFGHGRHSCPAQPFSLAAMTAAMTHL
                     LGRYEMTPEWTSHPRPVPAQIGGMARAAELCVIRYCATR"
     gene            complement(13079..>14926)
                     /gene="ppc"
                     /locus_tag="VFC01_15795"
     CDS             complement(13079..>14926)
                     /gene="ppc"
                     /locus_tag="VFC01_15795"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HZN81129.1"
                     /translation="ARLVTVSDDLAALADTCHEPARADEPYRRALRVIHGRLTATAAE
                     ILDSQPEHELDLGLERYLTPAELLADLDVIDRSLRANGSEVLADDRLARLREAVHVFG
                     FHLSGLDMRQNSDVHEEVVAELLAWAGVHPDYRSLPEPDRVELLAAELAIRRPLVKDG
                     AELSELARKELDIVAAGARAVHVFGEQAIPNYIISMCQSVSDMLEAAILLKEVGLLDT
                     SGGEPYAPVGIVPLFETIDDLQRGSSILEAALDLPLYRAVVAARGDSQEVMLGYSDSN
                     KDGGYLAANWALYRAELDLVESARKTGIRLRLFHGRGGTVGRGGGPSYDAILAQPPGA
                     VNGSLRITEQGEVIAAKYAEPRIAHRNLETLLAATLEATLLDVEGLGDAAGPAYEVLD
                     ELAARAQRAYAELVHETPGFVDYFKASTPVSEIGALNIGSRPTSRKPTTSISDLRAIP
                     WVLAWSQSRVMLPGWYGTGTAFETWIAEGDGRLEVLRDLYRRWPFFRTVLSNMAQVLA
                     KSDMGLAARYAELVEDDALRRRVFDKIVTEHDRTIRMHKLVTGHDDLLADNPALARSV
                     FNRFPYLEPLNHLQVELLRRYRSGDDDELVQRGILLTMSGLATALRNSG"
BASE COUNT         2649 a         4947 c         4912 g         2418 t
ORIGIN      
        1 cgtggtccac cacagccaga agaacgaggc caccgacaac gccagcccga acaccagcgc
       61 gtgacttcgc ggcacggtgt gctgggccaa cggccggcgg gacgtccgct tcatcacctt
      121 gtcgatgtcc gcgtcggcca cacagttcag cgtgttggcg ccggccgccg ccagcaggcc
      181 gccgaacata gtgttgacga tgagcagcgg atcaacagtg ccgcggtggg ccagcagcat
      241 cgccggaatc gtggtgacga gcagcagctc gatcacccgc ggcttggtca gtgagacgta
      301 gccaaggagc ttggtgatga ttcggttcgg cgcaacgcct tcgcgaatac tcacgcaaaa
      361 ctcctaaagg tcgccgcagc gcgcagcttc tactacagac gatggtagac cgcgtgtaaa
      421 cgccgtccta atcgtccccc acggacctgc ggcggcccag gttcgcgcac tagggtggac
      481 caagcggatt gcgaagccgg gcctatgtaa ggagttcgcc acgtgaccac tctcgacgag
      541 atctctacgc tcacccgccc gaatcacccg gacgactgga ccgagttgga ctcggccgct
      601 gtcgacaccg tgcgtgtgct ggccgcggat gcggtgcaga aagtcggcaa cggccacccc
      661 ggcaccgcga tgagcctggc gccgctcgcg tacacgctct ttcagcggca gatgcaccac
      721 gaccccagcg acacgcactg gctcggccgc gaccgcttcg tgctgtcggc cgggcattcc
      781 agcctgacgc tgtacatcca gctgtacctc ggcgggttcg ggctggaact gtccgacatc
      841 gaatcgctgc gaacctggaa gtccaggacg ccggggcatc ccgagttccg gcacacccca
      901 ggcgtggaga tcaccacggg ccccctgggt cagggcctgg cctcggcggt cgggatggcc
      961 atggcgtcgc ggtatgagcg cggcctgttc gatccggacg ccgcacccgg cacaagcccg
     1021 ttcgaccact acatctatgt gatcgcctcc gacggcgaca tggaggaggg ggtgacgagc
     1081 gaggcgtcgt cgctggccgg cacccagcag ctcggcaacc tgatcgtgtt ctacgacagc
     1141 aaccagatct cgatcgagga cgacaccaac atcgcgctga ccgaggacac gccggcccgc
     1201 taccgggcct acgggtggca cgtgcaggag gtcgagggcg gcgagaacgt ggtcggcatc
     1261 gaagaagcca tcgccgaggc caagcgcgtc accgacaagc cgtcgttcat cgcgatccgc
     1321 acgatcatcg gctaccccgc gccgaaactg atgaacaccg gcaaggcgca cggcgccgca
     1381 ctcggtgacg aggaggtcgc cgcggtcaag aagatcctcg gcttcgaccc ggacaagacg
     1441 ttcgaggtgc gcgacgaggt cctcgagcac acccgcaggc tggtcgcccg cggcaaggaa
     1501 gcccatgaga agtggcagag cgagttcgac gcctgggcgc agcgcgagcc ggaccgcaag
     1561 gcgctgctgg accggctgac cgagcacaag cttcccgacg gctgggacgc cgacctgacc
     1621 tactgggagc cgggttccaa agcgctggcc acccgcgcgg ccttcggtca ggtgctcaat
     1681 gacgtcgcgc cgaagctacc cgaattgtgg ggcggctcag cggatctcgc gggcagcaac
     1741 aacaccacga tcaagggcgt gaagtcgttc ggcccgccct cgatctccac caaagaattc
     1801 accgcggacc cctatggccg cgtgctgcac ttcggtgtcc gcgagcacgc catgggctcg
     1861 atcctgtccg ggatcgtgct gcacggaccg acccgcgcgt tcggcggcac cttcctgcag
     1921 ttctccgatt acatgcgccc ggccgtgcgg ttggcgtcgc tgatgaacat cgacaccatc
     1981 tacatctgga cgcatgactc gatcgggttg ggcgaggacg gtcccaccca ccagccgatc
     2041 gaacacctgg cggcactgcg cgccatccca aacctgtcgg tggtgcggcc gggcgactcg
     2101 aacgagaccg cctacgcgtg gcgcaccatt ctggcgcgcg gcgccagcag cggcccgacc
     2161 ggtttcatcc tcacccggca gggcattccg gtgctggagg gcaccgacgc cgaaggcgta
     2221 gcccgcggcg gctacgtgct gggtggcacg cccgatgagg atcctgatgt ggtgctgatc
     2281 ggcaccgggt cggagctgca gctggccgtg gctgcgcaga agatattggc ggacaaggga
     2341 atcaccgcga cggtggtgtc gatgccatgc gtcgagtggt tcgaatccca gccggcggag
     2401 tatcgcgaca gcgtgcttcc cccggcggtg tcggcgcgcg tcgccgtcga ggcggcggtt
     2461 gcgcagagtt ggtacaagct ggtcggcgac accggcgaga tcgtctcgat cgagcactac
     2521 ggcgaatccg ccgacgacaa gacgttgttc cgcgagttcg gtttcacccc cgaagctgtg
     2581 gtggccgccg cggagcgatc actagacaac tgatttggaa gggcgagaac aatgacccaa
     2641 aaccccaatc tcgcagcgct ttccgccgcg ggcgtgtccg tatggctgga cgacctgtcc
     2701 cggaaccggc tgcagaccgg caacctgcag gagctcatcg acaccaagtc tgtggtgggc
     2761 gtgacgacaa atccgactat cttccagggc gcgttgtcca agggcaccgc ctacgacgag
     2821 caggtcaagg agttggccga tcgcggtgcc gacgtcgatg ccaccatccg caccgtgacc
     2881 accgacgacg tccgcaacgc ctgcgacgtg ctggccaagg cgtgggaggc gtccaacggc
     2941 gtcgatggcc gggtgtccat cgaggtcgac ccgcgactgg cccacgagac cgacaagacg
     3001 atcctgcagg cagtcgagct gtggaaaatc gtcgaccggc ccaacctgct gatcaagatt
     3061 cccgcgacac tggccggtct tccggcgatc accgccgtta ttgccgaagg catttcggtg
     3121 aacgtgacgc tgatcttctc cgtccaacgt caccaggcgg tcatggacgc ctacctcagc
     3181 gggctggaga aggccaaaga ggcggggcat gacgtgtcca agatccattc cgtcgcttcg
     3241 ttcttcgtct cgcgcgttga caccgagatc gacaagcgac tggaaaagat cggcaccgac
     3301 gacgcgctgg cgctacgcgg tcaggccggt gtggcgaacg cgcggcttgc ctacgcggcc
     3361 tatgaggaag tgttcgtcgg cggttcccgc tacgaggctc tgaaggccga aggcgcacgt
     3421 gtccagcggc cgctgtgggc gtcgaccggt gtcaaaaacc cggagtactc ggacacgctc
     3481 tatgtcaccg agctcgttgc accgaacacc gtgaacacca tgccggagaa gacgattgac
     3541 gcggtcgccg accacggtgt catcgcgggc gacacggtca ccggcaaggc cgcagaggcg
     3601 caggcgacct tcgacgagct agacgcggtg ggcatcgatc tgccggatgt gttcaaggtg
     3661 ctcgaggacg agggagtgga gaagttcgag aagtcgtggc tcgaactact cgaagcgaca
     3721 caaggtcagc tcgacgccgc caaaaaatga ccgctgtcaa cgataagtca acatcgacaa
     3781 gctggcgtaa cccgctgcgc gacaagcgcg acaagcggat gccgcgcatc cccggtccgt
     3841 gcggtgtggt gatcttcggc gtcaccggcg acctggccac caagaagttg atgccggcga
     3901 tctacgacct ggccaaccgc ggcctgctcc cggcgtcgtt cgccttggtc ggtttcgcac
     3961 gacgggattg ggccgacgag gatttcggca agctcgtctg cgacgccgtc cgaaaacacg
     4021 cgcgcactcc gtaccggcaa gaggtctggg accggctggc cgagggaatc cggttcgtac
     4081 agggcacctt cgatgacgac aaggcgttcg aacggttggc cgagacgttg gcgaagcttg
     4141 atgccgagcg gggcaccggc ggcaaccacg cgttctatct gtcgattccg cccaaggcgt
     4201 tccagcaggt ctgcgagcag ctacagaagt ctgggttggc ccggcctcag gatggccgct
     4261 ggagccgggt ggtcatcgag aaaccgttcg gccatgattt gcaaagcgcc caagagctca
     4321 acggggtcgt caacagcgtc ttccccgagg agtcggtgtt ccgcatcgat cactatctcg
     4381 gcaaggagac agtccagaac atcctggcgc ttcggttcgc caatgagctg tacgaaccga
     4441 tctggaacaa caactacgtc gacagcgtgc agatcaccat ggccgaggac atcggcctcg
     4501 gtggccgggg tgggtactac gacggtgtcg gtgcggcacg cgatgtgatc cagaatcatc
     4561 tgctgcaact acttgccctg accgccatgg aggagccggt caacttcagc ccgcacgagt
     4621 tgcaggcgga gaagatcaag gtgctttcgg caatccggct gatgcagccc ctcgatgaca
     4681 ccaccgctcg gggacagtac ggggcgggtt ggcagggtag cgagaaggtc gtcggtctgc
     4741 tggacgagga aggcttctcg aaggagtcca cgaccgagac ctacgcggcg atcacgctgg
     4801 agatcgacac ccgccgctgg gcgggtgtgc cgttctttct gcgcaccggc aaacggctgg
     4861 ggcgcagggt gaccgagatc gcgctgatct tcaagcgcgc cccgcacctg ccgttcgaca
     4921 agacgatgac cgaggaactc ggccaaaacg cgttggtgtt cagggtgcag cccgacgaag
     4981 gcattacgac gcggttcggc tccaaagtcc ccggaagcgc gatggaggtc cgcgatgtca
     5041 acatggactt ctcctacggc tcggcgttcg ccgaggattc ccccgaggca tacgaacgac
     5101 tgattctcga catgctgctc ggcgagccct cgctgttccc ggtgaaccga gaagtcgaat
     5161 tgtcctggga gatcctcgat cccgtgctgg attactgggc gtcgcatggc aagccagaca
     5221 cctacgaatc aggcacctgg ggaccggcgt cggccgacga gatgatgcac cgcatgggca
     5281 gggaatggag gcggccgtga ttcgcgaggg agccgagcaa tgatcgtcga cctgcccgac
     5341 accacgacca acgacatcaa caagaagatc acgggccttc gcgaagaggg tggcgcgatc
     5401 acgttgagcc gagtgctcac gttggtgatc tcgcttgact ccgacgatgt gctggaagat
     5461 tcaatcgagg cggcgaactt cgcgagccgc gaacacccct gccgtgtgat cgtggtggtc
     5521 caaggcgatc gaagcgcctc cgagccgcga ttggatgcgc aattgcgcgt cggcgctgac
     5581 gcgggtgcgg gcgaggtcgt cgcgctgcgc ttgcacggtg agctcgccga ccacgccagc
     5641 agcgttgtgc tgccattcct gctgcccgac accccggttg tggcatggtg gccggtgggg
     5701 ggaccggcgg ttccggcaca ggacccattg ggccacttgg caattcgccg catcacaaat
     5761 gcaacggcct gcgaggaccc gctggcagcg atcaaaggcc gcctacaggg ctacaccgac
     5821 ggcgacaccg atctggcctg ggcccgcatc acatactggc gcgccctgct ggcgtcggcg
     5881 ctcgatcagc cgccctttga gccggtcacc tccgcggtgg tgtcaggact gagggatgag
     5941 ccgtcgctcg acgtcctcgc gggctggctg gcggtccgga tcgacggtcc ggtacagcgc
     6001 gcagtgggtg aactgaaggt ggaactcatc agaagcagcg agaccatcac actgaagcgt
     6061 ccgcagacag gggtgaccgc gacactgacc cgaaccagca ggcccgaggc acggattccg
     6121 ttggcgcgca gggaagccaa ggaatgcctc gccgaggacc tgcgcaggct ggacgccgac
     6181 gagatctatc acgaggcgct gcagggcatc gacaaggtcc agtacacatg agctccgaag
     6241 gcgacgacga gcgaggaccg gagcgaggtg ggggcacctc ccacccgcag ggtaggggga
     6301 gagtcgaggc atgaactccg tcatcgagaa atacgccgac agcgctgcgt tggtcgccgc
     6361 ggcaggcgat cgactagtcg acgcgatcac cgacgcaatc gacaaacgcg gcacggcgca
     6421 gatcgtcctg accggcggcg gcaccggcgt cggcctgctc aagcgggtcg gcgagcgcgg
     6481 cgagaagatc gactggtcga aggtccatct gtactggggt gacgatcggt tcgtgccggc
     6541 agacgatgac gaacgcaatg acaaacaggc cgccgacgcg ctgctcgacc acatcgacat
     6601 cccggacgcc aatgtgcatc cgatgtcggc cagcgacggc gagttcggcg acgacctgga
     6661 cgccgcggca cgggactacg agcaggtgct cgccgcgaac gccgatgacg gcgagcccgc
     6721 accggacttc gatgtccatc tgctcggtat gggcggcgag ggacacatca actcgctgtt
     6781 tcccgagaca cccgcggtgc gcgagacgag ccgactggtg gtcggtgtcg aggactcccc
     6841 caaaccgccg ccccgccgaa tcacgttgac gctgcccgcg gttcagcgtt cgcgcgaagt
     6901 gtggctcgtg gtatcgggtg ccgccaaggc cgatgcggtg gcggctgcga tcggcggcgc
     6961 caaacccgtc gacgtccccg ccgcaggcgc tgtcggccgg gatgcgacgg tgtggctgct
     7021 cgacgaggac gccgcggcca agctctagta cgacgggact cgggcgtcga ggccggtcag
     7081 gacctccggc aacgggtcgc ggtgcagcac tccgagccgt tgtgtggcgc gggtcagggc
     7141 gacatagagg tcggcggcac cgcgtgggcc gtcggcgagg atgcgttccg gctccacaac
     7201 cagcactgca tcgaactcca ggcccttcgt ttccgagggc accaccgcgc ccggcacatc
     7261 cggcggtccg atcacgatgc tggtgccttc gcggtcagct tcgtcaatga cgaattcttc
     7321 gatggcagtg ggcaattcgt cgtcggtaac ccgttgcgac catggctgaa tcccacaagc
     7381 gcggaccgat tccggtggct cgaccccggg cgcgaactcg gcgagcagcg cgcccgcgac
     7441 agtcatgatt tccgccgggg tgcggtagtt caccgacagc gaccggtaca cccaccggcc
     7501 tggcacgtac gcctcaagca tcgtgtccca cgacgtcgcg ccggacatcg accggcgttg
     7561 cgccagatcg ccgaccaccg tgaaggatcg gctcgggcaa cgccgcatga gcacgcgcca
     7621 gtccatttcc gatagctcct gtgcctcgtc gacgacggcg tggcggtagg tccagtcgcg
     7681 gtccgcggcg gcgcgttcgg cgagttcacg ggtgtcgcgc tccgcgaagc gatcagccag
     7741 gtcctcggcg tagatcatgt ccgtcgcgag caggtggtcc tcgtcgtcca tcaggtcctc
     7801 gcggctgacc atgaggtcga gcacacccgc cgcgtactcc gcttcggcca gccgttcccg
     7861 atcggcggac tggtctgccg gcttgtcgcg gcccagcaga tcgacgagtt cgtcgagcag
     7921 cggcacgtcc gagaccgtcc aggcgtcgcc atcggcgcgc catagcgccg ggtcggcacc
     7981 cgccgcgcgc aaccgctcgg gagactcgta gagctgcgcc aacaacgttt ccggcgtgag
     8041 tatcggccag agttcgtcga gcgccgcggt gaactggtcg ttgtccgcta gctcgtcgat
     8101 caggtcagcc cgcaggtgct cccatgcgtc gcggtccttc cgggtcagcc agccagcgcc
     8161 gatccgcgcg atcgctcgct cggtgagtac gtaggtgacg atctcgcgga acactgcgcg
     8221 ggcttcgttg tgcggctgcc cgctcgcgcg cgcctcctgg atggcccact ccgcggtctc
     8281 ggcgtcgatc cgcaccgtga catccgacaa tttgatcgac agcggatctt ccggcaaccg
     8341 ctgccgatcg gcgaccgccg ccgcaagcac ctccagaatc ttcagtgagc ccttgagtcg
     8401 cgcggcctcc gccgtgtcct cggcggtgat gtgcagacct ggcacgaggt cgccggtggt
     8461 catgaacaca acgttcgtct cgcccagcga tggcagcacg cggccgatgt ggttcaggaa
     8521 cgccgtgttc gggccgacga cgagcacacc atgacgttcg atccgctcgc gctgggtgta
     8581 gagcaggtat gccacgcggt gcagtgccac cacggtcttg ccggtgcccg gcccaccctc
     8641 gattaccagc acacccgaat gatcgagccg gatgatctcg tcctgttgcg cctggatcgt
     8701 cgcgacgatg tcgcgcatcc cgtcgctgcg cggcgcgttg acagccgcca gtaatgcggc
     8761 gtctccacgc tggccgtcgc ccggcctgcc gaacatctcg tcggtgaagt cgacgacctg
     8821 acgacccctg gtgtggaatt ggcggcgcct gcgcatgttc tcagggctgg ccgcggtggc
     8881 gacgtagaac gcgcgcgctg caggcgcccg ccaatcgagg agcagcggtt cgtagtcgtt
     8941 ctgctcgtcg aacaggccta tgcggccgat gtacaaacgt tcgccggaaa ggctgtccaa
     9001 ccgcccgaaa cacaacccgt ggtccgccac gttcagccgg ttcagctgtt tggccagcgc
     9061 acggacctcg acatcgcgct ccacgagtgg ggcgccgtta ccggcgtcga cgggacttcg
     9121 cagcgccacg ccgtacctac cccgcacgcg cgcgcgctcg gcgtcgagcc gcgcgtagag
     9181 cccggccacg tagccacgct cgccctgcaa ttcctcgtcg tagcttcgag ttgacacacg
     9241 tcccccatct tttccggtct aagccagcga ttttggggca tcacccgggt cttgccgcaa
     9301 gtccccccat gcgttataag ttggaagtgg caaggagttg atagcgctcc ttgcctttct
     9361 catgcattcg ggtgaggcac atggcaagtc ttagccgtaa ggcattgtcc cacaatttga
     9421 ttgagcctta ccggctgccg atgatgtcac cgatatacgc gtgacgaacg accgaaggcg
     9481 gttcgtctgt gcagcgacga cgatcaccgg gaagttgggg aggaaggcga cagcagcggg
     9541 gactctgcga ccgcattcat atcgcgtggt cgttcgcgct actgcccgac tcacatgagc
     9601 aggagaactc ccaatccccg ggcctggagc cggttaaatc gccatagcag cagaactgaa
     9661 tcttcacacc gtgctatacg cgcaggcgaa cggcgcctcc cgaatccacc gacccatcac
     9721 cggggtaccg gccggcttgt ggacaacttt tcgacttgtc ggcgccttgt ggcattgtcg
     9781 aaagtatgtt cgaagatgtt ccggatggcg agctcattga tgtgatgggt gaggccaccc
     9841 gggatgagtc gacggcgatc gcgcagcggt tggccgcggt cgcgcagttg tatgtccggc
     9901 gctccgggga gttggctgat caccagtggt ggtgtgccga tgcgtgtgat gcggtggccg
     9961 cagagatctc ggcggcacaa aacatcagcc atgcccgcgc ggtggctcag gtgcagttcg
    10021 cgtgcgcggt gcacgatcgc ctgccgcagg tggccaaggt gttcgccacc ggggtgatcg
    10081 actttcggtt ggtgcgtacc ctgatcgccc gcaccgagaa cgtcgacgat gcggtgatgc
    10141 ccgagctgga tgcggcgatc gcccggcatt gccagaagtg gatgaagctc tcgggcccga
    10201 aggtgcgcga tcgggtggat atgtgggtcg ccaagttcga ccccgccggg gtgcgggtgc
    10261 cgccgacggt cgaggagaac cgctatgtcg aggtcggcca gaccagcccg gggatggccg
    10321 gactctcggg ccacgtgcac gccaccgacg ccgcggtgtt cgaccgacgg ctcgacgcct
    10381 tggcggacac ggtgtgcgag aacgatccgc ggaccaaagc gcagcgccgt gccgatgcca
    10441 ccggagcggt gggccgcggc gaggccacgc tggcctgcca atgcggatta gcggactgcc
    10501 ccgccagagc cgagcgcgac ggtgcggccg ccgcggccgc agcggtgatt cacgtgctgg
    10561 ccgaacgggc caccctcgac ggcgccagcg atcagcccgg ctatctgccc ggtttcggtg
    10621 tgctgcccgc cgagtcggtg cgcgaggtcg ccaagaccgc gcgactcaag ccggtggtca
    10681 tcccgtcgga cacccccgat ccggggtatc gaccctcggc ggtgacgcgg gagttcctgg
    10741 tgtggcggga tttgacgtgt cgctggcccg gctgcgacag acccgtcgag aaatgcgacg
    10801 tcgaccacac cgtgccctac ccggccggac tcacccatcc ctcggatctc aagcattact
    10861 gtcgcatcca tcatctgatc aagacgttct acggcggccc gggcggctgg accgatcagc
    10921 aaatgcccga tggcacagtg attctcaccg cacccaccgg gcacagctac accaccgagg
    10981 cacacggcgc ccgcatgttt cccgcgctgg cccaatccac cggcgagttg cccatcccgc
    11041 cggcactcga tctgcctgac accgaccgat cggtaatgat gccgcgccgc aagcaaaccc
    11101 gggaacaaga ccgccgcgac cggatcaacg ctgaacggcg cgaacgaacc gaactcatcg
    11161 ccgaagaaga acgccaacgc caagcctggc tcgccgcgaa ctacgaacca ccaccattct
    11221 gagggtagtc gtaactcctt attccgccgt cggtttcacc gtcgcgagac ccttgcgcgt
    11281 actcgtcggt ccgcgtcgtt gggtcggttc cgcctgttcg ccaccgaggg tcggcgcggc
    11341 attggcgggg cggcgcatcc cctaggtaat ccctcacaac tcgttcagac gaccagcccg
    11401 gtccggcggt tccaacggcg cagtcataat gaccgtcatg gcagacaagg gtggcaagcc
    11461 agcacgcact ggatcggagc ggatcaggaa gctcgcgcag gcggcgctca acgccgacgt
    11521 cacggtggag caggttgaca ccatcttgga gggcctgagc gagacgctgg tcgatctgaa
    11581 caactcgacg ggcaatctcg acgcaaccct ggagcggttc aacgaaacga tcaaccagat
    11641 caacgagctg gcgccgcgcc tgaacgccgt ggttgatcgc atggagggca tcgtgtcccg
    11701 cgtcgaacgc atcgtcggga taggcgaggc cgtggtgtca ccactgacgg ccaccgagca
    11761 agccgtgcgc ggagccgtga acgccgtgcg gcgaagcacc ggcttgtagt gacgcgcggc
    11821 tcgatcagtc ccgcgccgcc ggtcgccgag ggcaccgggc tgccgtggga tgtgacggtc
    11881 gacgacgccg tcgccgccat cgccgaagcc cgcgctcggc acggcgacac ctttgtggcg
    11941 cacagcggtc gcgaccacta tctcttcacg ttctcccccg tcggcgtcga gtcgttctac
    12001 gcgctgcccg aggagatcgc gagcaagggc ctcgccgact atttgatgtt gcggcgcaag
    12061 ctccccgacg agatcttcga cggcaggcgc accctacccg cctcgttgtt ccggcgcgac
    12121 gacgtcacct cttatctggt caacctcgac caggcgctcg agcagaccgt ggctgagctc
    12181 ggccccgaag gcaccgtcga tctgttcgac ctcacccgca ggctcgggca ccgcatggga
    12241 cttgcctcgt gggcggggcc tggatcgacg caagccgata ccttcgatcg gctggttgcg
    12301 gccttcgaca tcctcgacgg gtcggatgcg ttcgtgcatc ccgacgcgat ggcggccgtc
    12361 gcggcgacgg acaagcgcgc cgagcgggcg gcgctcgacg acgtcgtcga actcgtctcg
    12421 atcgcggtgc gacgcttgga ctccggtcag acaacagacg acgcactgtt cggccggatc
    12481 gtcgaggcct ggtcgtcgga accgcccgac atccgcacgc gcggaatcgc attggacgtc
    12541 gcgctcatcc acatcgcatc gatgtcgaat ctcatggcgg ccctcggctg ggccttcgtc
    12601 gatctgctcg agcacccgac acacctgcgc ctcgtcgctg ccggcgagga ggaactggcg
    12661 cgccgctgcg cgctggagag cacgcggttg gcgcagcgct cgatcatgtc gcgcaccgtg
    12721 ttggctcccg tcgatttcga cacaggcgac gcgtgctacc gcgtgcccgc gggctggacg
    12781 atcgccacac tgctgccgct gctgaacacg tccgccgcgc cgggactacg ggactgggac
    12841 cccgaccgat ggcaccgtca tcggcttgcc gacccgaccg cgctcccttc acccatgctc
    12901 gtgacggcgt tcgggcatgg caggcactcc tgccccgcgc agccgttctc cctggcggcg
    12961 atgacggccg ccatgactca tttgctcggt cgctatgaga tgacgcccga gtggaccagc
    13021 catcccaggc ccgtgcccgc tcagatcggc gggatggcgc gagcggccga gctctgcgtt
    13081 atccgctatt gcgcaacgcg gtagccagcc cactcatcgt cagcaggatg cctcgctgga
    13141 cgagttcgtc gtcatcaccg gagcggtacc gccgcagcaa ctcgacctgc aggtggttca
    13201 gcggctcgag gtacggaaac cggttgaaca ccgaacgcgc cagcgccggg ttgtcggcca
    13261 gcagatcgtc gtgcccggtg accagcttgt gcatccggat ggtgcggtcg tgttcggtga
    13321 cgatcttgtc gaacacccgc cgccgcagcg catcatcctc taccagttcg gcatagcgcg
    13381 cggccaggcc catgtcggat ttggcgagca cctgcgccat attcgacagc acggtgcgga
    13441 agaacggcca ccgccgatag agatctcgta gcacctccag ccggccgtcg ccttcggcga
    13501 tccaggtctc gaacgcggtt ccggtgccgt accagccggg cagcatcacc cgcgactgac
    13561 tccaggccag cacccacggg atggcccgca gatcggagat cgacgtagtg ggcttgcgcg
    13621 aggtcggtcg gctgccgatg ttgagcgcgc cgatctcgct gaccggggtc gaggccttga
    13681 agtagtcgac gaaacccggt gtctcgtgca ccaattcggc gtatgcgcgt tgagcgcggg
    13741 ccgccagctc gtctagcacc tcgtacgcgg gacccgcggc gtcacccaac ccctcaacat
    13801 cgaggagcgt ggcttccaac gtggccgcca acagcgtctc caggttgcga tgcgcgatcc
    13861 gcggctcggc gtacttggcg gcaatcacct cgccctgctc ggtgatccgc agcgacccgt
    13921 ttaccgcccc gggcggctgc gccagaatgg cgtcatagct cggacccccg ccgcgcccga
    13981 ccgtcccacc gcgaccgtgg aagagccgca accgaattcc cgttttgcgg gccgattcga
    14041 ccagatcgag ctcggctcgg tacagcgccc agttggccgc cagatacccg ccgtccttgt
    14101 tcgaatcgga gtagccgagc ataacttcct ggctgtcacc gcgcgccgcc acgacggcgc
    14161 ggtagagcgg aaggtccagc gccgcttcga gaatcgacga gccgcgctgc aggtcgtcga
    14221 tggtctcgaa cagcgggacg atgcccacgg gcgcatatgg ctcgccgccc gacgtgtcga
    14281 gcagtccgac ctccttgagc aaaatcgctg cctcgagcat gtccgatacc gattggcaca
    14341 tcgagatgat gtagttcggt attgcctgtt ccccgaacac atgaacggcg cgcgcaccag
    14401 ctgccacgat gtccagctcc ttgcgcgcca gctccgacag ttcggcaccg tccttgacga
    14461 gcggccgacg gatcgccagc tcggcggcga gcagctcgac gcggtcgggc tcgggcagcg
    14521 accgataatc gggatgcacg cccgcccacg ccaggagttc ggcgacgacc tcctcgtgca
    14581 cgtcagagtt ctgccgcata tcgaggccgg acaaatgaaa cccgaagaca tgcacggctt
    14641 cccgcagcct ggcgagccgg tcgtctgcga gcacctcgct gccgttggcg cgcaatgacc
    14701 gatcgatgac gtcgaggtcg gccagcaact cggccggtgt gaggtaccgt tcgaggccca
    14761 ggtcgagctc gtgttcgggc tgactgtcca ggatttcggc tgctgtcgca gtcagccgtc
    14821 cgtggatgac gcgcaacgcg cggcggtagg gttcgtcggc gcgggccggc tcgtggcatg
    14881 tgtcggccag cgcagcgagg tcgtcgctga ccgtgaccag ccgcgc
//
LOCUS       DASRJA010000356         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3167805,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000356 DASRJA010000000
VERSION     DASRJA010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3167805"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VFC01_15800"
     CDS             <1..1129
                     /locus_tag="VFC01_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HZN81130.1"
                     /translation="ATGAIAKRANEILRDGLKGVKRLPLARALQSAQRHDYMDAYVED
                     LPSVVDVHAIRAEGIRVGADPLGGASVDYWGAIAERHNLNLTVVNPLVDATWRFMTLD
                     TDGKIRMDCSSPNAMASLIANRDKYQIATGNDADSDRHGVVTPDGGLLNPNHYLAVAI
                     EYLYTHRPDWPGSTAVGKTAVSSSIIDRVVAGLGRKLLEVPVGFKWFVDGLISGTIGF
                     GGEESAGASFLRRDGSTWTTDKDGIILALLASEILAVTGQTPSQRYDALAEKYGAPTY
                     ARIDAPADREQKARLAKLSPEQVTATELAGEEITAKLTTAPGNGAPLGGLKVTTENAW
                     FAARPSGTEDVYKIYAESFKGPEHLAEVQESAKEVVNKVIA"
     gene            1126..2388
                     /locus_tag="VFC01_15805"
     CDS             1126..2388
                     /locus_tag="VFC01_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN81131.1"
                     /translation="MSSVAEGECSADSKPKLPSEVWVLITANVVVALGYGVVSPVLPQ
                     YARNFGVSISAATFVITAFSLMRLVSAPASGLFVQKAGERRVYVSGLLIVAVSTGACA
                     FAQTYWQLLLFRSLGGFGSAMFTVSSLGLMIRISPPDARGRVAGLFSSAFLIGSVAGP
                     VLGSLTAGLGLAAPFAIYGVALLIAAAVVFINLRHSAVAAPAENAEDPVTLRIALRNR
                     AYWSALFSNFAMGWSVFGLRIALVPLFVTEVLHRGPRTAGLALATFAVGNVAAVIPSG
                     HLSDRIGRRALLIVGLAVSGVATMAVGLASSLTLFLAGALVAGAAAGMFTSPQQAAVA
                     DIIGSKARGGTAVATYQMMADFGAIVGSLAVGEIAQHVSFGAAFVISGAILLVGGVGW
                     IFAPETRLRPSEPTPARALGPEVGGELP"
     gene            2445..2517
                     /locus_tag="VFC01_15810"
     tRNA            2445..2517
                     /locus_tag="VFC01_15810"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2478..2480,aa:Ala,seq:ggc)
BASE COUNT          415 a          819 c          932 g          467 t
ORIGIN      
        1 cgcgaccggg gccatcgcca agcgggccaa cgagatcctc cgcgacgggc tcaagggtgt
       61 gaagcggctg ccgctggccc gggcgttgca gagtgcgcaa cgccacgact acatggacgc
      121 ttacgtcgag gacctgccca gtgtggtcga cgtgcacgcg atccgcgccg agggcatccg
      181 cgtcggcgcc gacccgctgg gcggagccag cgtcgattat tggggcgcga tcgcggaacg
      241 ccacaacctg aacctgacgg tggtcaatcc gctcgtcgac gccacctggc ggttcatgac
      301 gctcgacacg gacggcaaga tccggatgga ttgcagctcg cccaacgcga tggcatcgct
      361 gatcgcgaac cgagacaagt atcagatcgc caccggcaac gacgcagact ccgaccggca
      421 cggcgttgtc acccccgacg gagggttgct gaacccgaac cactacctcg cggtggcgat
      481 cgagtatctg tacacccaca ggccggactg gccgggttcg accgcggtcg gtaagaccgc
      541 cgtcagctcg tccatcatcg accgggtggt cgccggcttg ggccgcaaac tgctagaggt
      601 gccggtcggg ttcaagtggt tcgtcgacgg attgatcagc ggcaccatcg gtttcggcgg
      661 cgaggagagc gcaggcgcgt cgttcctgcg ccgtgacggg tcgacgtgga cgaccgacaa
      721 ggacggcatc atcctggcgc tgctggcgtc ggagatcctt gcggtgaccg gccagacccc
      781 gtcgcagcgg tacgacgcac tggccgaaaa atacggtgcg ccaacgtatg cccgcatcga
      841 cgcacccgcc gaccgggagc agaaggcgcg gctggccaag ttgtcgccgg agcaggtgac
      901 cgcgaccgaa ctggcgggcg aggagatcac cgccaagctg accaccgcgc ccggcaacgg
      961 cgcaccgctg ggcgggctga aggtgacgac ggagaacgcg tggtttgcgg cacgcccgtc
     1021 gggcaccgaa gacgtctaca agatctacgc ggagtcgttc aaggggcccg agcatcttgc
     1081 ggaggtccag gaatccgcca aggaagtggt gaataaggtc atagcgtgag ttctgtcgcg
     1141 gagggtgaat gttcggctga ctcgaagccg aaactcccga gcgaagtctg ggtactgatc
     1201 accgcaaatg tggtggtcgc cctcggctat ggcgtcgttt cgccggtgct accgcagtac
     1261 gcgcgcaact tcggtgtgag catcagcgcc gcgaccttcg tcatcaccgc cttctcgctg
     1321 atgcggctgg tgtccgcgcc ggcgagcggt ctgttcgtcc agaaggccgg ggagcggcgc
     1381 gtctatgtga gcgggctgct gatcgtcgcg gtgtccacgg gcgcgtgtgc cttcgcgcag
     1441 acgtactggc agttgttgct gttccgctcg ctcggcgggt tcgggtctgc gatgttcacc
     1501 gtctcgtccc tggggctgat gatcaggatc tcgccgcccg acgcccgcgg ccgggtcgcc
     1561 ggattgttct cgagcgcatt cctgatcggg tcggtcgccg gcccggtgct cggaagcctg
     1621 accgcagggc tgggcctggc cgcgccgttc gccatctacg gtgtggcgct gctgatcgcc
     1681 gcggccgtgg tgttcatcaa cctgaggcat tcggcggtgg ctgcacctgc ggagaacgcc
     1741 gaagacccgg taacacttcg aattgcgttg cgcaacagag cgtattggtc ggcgctgttc
     1801 tccaacttcg cgatgggctg gtcggtgttc gggttgcgca ttgcgttggt gccgttgttc
     1861 gtcacagaag tgctgcatcg tgggccgcgt actgcggggc tcgcgttggc gacgtttgcg
     1921 gtcggcaacg tggcggccgt catcccgagc ggccatctgt cggaccgcat cgggcgccgc
     1981 gcattactga tcgtgggcct ggcggtgtca ggggtggcga cgatggcggt gggcctggct
     2041 tcgtcgctga cgctcttcct tgccggtgcg ctggtggccg gcgcggcggc gggcatgttc
     2101 acctcgcccc agcaggccgc tgttgcggac atcatcggca gcaaggcgag gggcgggacg
     2161 gccgtcgcga cctaccagat gatggcggac ttcggcgcga tcgtgggctc actcgcggtc
     2221 ggcgagatcg cgcagcacgt gagcttcggc gctgccttcg tgatcagcgg agccattctg
     2281 ctggtcggag gggttggctg gatattcgca ccagagaccc ggctgcggcc ctcggagccg
     2341 acccctgctc gcgcgctcgg cccggaggtg ggcggcgaac tgccctgacc agcggttttg
     2401 aacgcttccc gcaggtggtg taacttcgat gagcacaact tcacggggct atggcgcagc
     2461 tggtagcgca ccacactggc agtgtggggg tcaggggttc gagtcccctt agctccactt
     2521 tttcggacac caagcgtccg tagacgaacc gtgacgtgtc tctacgaggt cggagacact
     2581 ctttgcgacg ttcgtgcgac cgggcaagcg cggcgacatg acggcgcata gaa
//
LOCUS       DASRJA010000357        19876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3152762,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000357 DASRJA010000000
VERSION     DASRJA010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 19876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19876
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3152762"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFC01_15815"
     CDS             complement(<1..219)
                     /locus_tag="VFC01_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN81132.1"
                     /translation="MQRVHYDADHRAFAELAGDFAAKEVAPNIAEWEADGIIPREVFA
                     KAGAVGLLGFAADERFGGVGARDFRYNQI"
     gene            322..2208
                     /locus_tag="VFC01_15820"
     CDS             322..2208
                     /locus_tag="VFC01_15820"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN81133.1"
                     /translation="MTTDAELLIHLRQADPGVLVAVLAQLTGDASVIDRFGPAISYVP
                     DPPERAGVTDPDTADALVAAVIEALDAPRFAGAPATVDPDLFTRLLPLALGSDVDDEF
                     VPLLLEQGGFQLSQPTLPRTVPIPETFKVAIVGAGLAGIIAALAAADAGVAYEIFDRN
                     NEVGGTWLTTTYPGIGVDTPAAYYSLSREVNPDWSSYYPQGGEYQSYLVALADKYGLR
                     EHTRFGTEVEALWWDEDRDHWQIHSVDRDGNRDVSFARVVITAAGYLNRPRLPDLPGR
                     DIFAGISIHSALWDASLDLTGKRVAIIGAGCTAVQIVDACVDQVDHLTVFQRQPHWVA
                     PRKRLSDDVPEHRRYLGRHLPHYAKWNRLKSFWGTADNNYPVILRDAEWAKTHLSISP
                     ANDVLLQMCLEYIDRTFGEGTELAKKVTPDFAPYGKRIIRDPGGYYAALTRNHVDVEA
                     SEPAEVNADGIVTADGRQIDLDVIIYATGYHLDFLSTIDIRGRGGRSLTDEWGDSPRA
                     YRGGTVPGFPNLFINSAPNYSPGHGAGANFSMEVMAHYIVECLQLMALRGATTMEVTQ
                     QAYDEYVDGIDEAMAGTVWCHTPSAHTYYRSGSGRVVVATPYRLVDLWHQHRAPIEEH
                     FELL"
     gene            2205..3092
                     /locus_tag="VFC01_15825"
     CDS             2205..3092
                     /locus_tag="VFC01_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN81134.1"
                     /translation="MTQLLSGKTALVTGSSRGIGRAIAQRLAAESATVVVTARSYEPS
                     ASVRGGEALAIPGTIRETVELIEEAGGKAFGVAADLEDTEQREGLVKKVLDRTGRIDI
                     LVNNAGFADYGLVEHMEMDTFDRTVEHYLRTPFVLAKAAVPHMRKQGAGWIVNIGSVT
                     GVAPMRPYREYNKTSGDVVYASMKAALHRFTQGVAAELVDANIAVNCVGPSTAIRTPG
                     AAQLIPDTFPTEPVEYLAETVLAMCFLPAAERTGLVAFSLHYPWSQKLTVHSLDGASV
                     LPPLEPPVTANPNILSAGM"
     gene            complement(3185..4114)
                     /locus_tag="VFC01_15830"
     CDS             complement(3185..4114)
                     /locus_tag="VFC01_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81135.1"
                     /translation="MNSELNYDDYTHDFMGKVGNIEDFSRNFLLGLARVFEDTLNFVP
                     YAHVKIYAEKVGINAAMDIAAEVSRAGMRQAMPMGRFIAPPGWDWKNAQYQAIPFDVQ
                     TEDLTKDALMRLIKTYWDQYLKGHNYFIDQWTKIIPEEEVWLGLPGLYELIIDYQYPK
                     LAKVMKIEPVYVVDFLKLACLSIDGTLGYGGEYIVHNPDHVVLNMRRCEVLQKYTDEG
                     LYPPARAWMNCTFEQRISAPFFPGCKLDINLPPKDFKFAEGEPFCVWTYTRGADNHAA
                     ASAAPDPRASAMKKIPIMPVSSDAAPSASETAT"
     gene            4331..4957
                     /locus_tag="VFC01_15835"
     CDS             4331..4957
                     /locus_tag="VFC01_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN81136.1"
                     /translation="MTIALTARDIFLADGSTSATVERICEAVGIAPRTFHRHFPVKED
                     VVMPLFRRFGGLSVEVLQDAPRTSDTVETLVKAFSTEVAKRGELEIDRKFMSLMITDP
                     QYRLRWLDWGQDFADPISEFLAERVDLGGDPFIRTLPAQLIIQICRQAYVHWVAQGNF
                     RSLESALRAGMLMVIRALPPLTPEAVAAVKSGPTRASRKRYGTRGRER"
     gene            complement(4896..5522)
                     /locus_tag="VFC01_15840"
     CDS             complement(4896..5522)
                     /locus_tag="VFC01_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HZN81137.1"
                     /translation="MYSVTRMLDVAEVDRDRLLAAARAGAAATRARHQLVAPTLPGVR
                     NGGDILVHLRFDSEDQWRSVETDFAVTLTDSAITRVNGACYSGDASVRGSHTGTVYRA
                     LLLRVLPGTDAHIVARFEDELRLMPRYVNTITAWQLSRVDNPVGTSEWTHVFEQEFTD
                     VDGLMGPYLMHPIHWAVVDRWFDPECPDVIVRDRVCHTFCAMPESVLT"
     gene            complement(5515..5862)
                     /locus_tag="VFC01_15845"
     CDS             complement(5515..5862)
                     /locus_tag="VFC01_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81138.1"
                     /translation="MTGSEVYVIDRVVTRPGCARKFVDCYLAEYAPGARARGMTLRDV
                     LVSPPIWFDDQSNTVTITWSLTSAQAWWEMTWKGRPDPTLGPWWDGIGELVEERTRSV
                     AAAAADVDRLCDV"
     gene            complement(5859..6845)
                     /locus_tag="VFC01_15850"
     CDS             complement(5859..6845)
                     /locus_tag="VFC01_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN81139.1"
                     /translation="MSLDPQIAEIIEALDAGFPPVHTMTGAEARASIRSRFVASSTPE
                     EVGEIRDASVHGPAGDVAVRIYRPASMSGPVPTLVYAHGGGFVFCDLDSHDGLCRSLA
                     NLIPAVVVSVAYRLAPEYRWPAAAEDMFAVTQWAARNADALGGDAGRVVVGGDSAGGN
                     LAAATALMARDRGAPSLVAQLLLYPMIAPNFDTESYRMFGKGFYNPRPALQWYWDQYV
                     PSAADREHPYASPLNADLRGLPPAVVVIAGHDPLRDEAVAFGNALEAAEVLTVQCRYD
                     GGIHGFMTMPALDLAQQARVETCRELSRLLTTAPVYRDAALPATTLRRTLAT"
     gene            complement(6842..7654)
                     /locus_tag="VFC01_15855"
     CDS             complement(6842..7654)
                     /locus_tag="VFC01_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN81140.1"
                     /translation="MTVTVETTTPSAVIDRVSLVLDAFDGPGRLTLAQIVRRTGLPRS
                     SAHRMLERLVQLRWLRRSGRDYELGMRLVELGSLAVHQDRLHRAAIPLLHDLHRATGL
                     VVHLAVLDGSDVVYLEKIGDRMTAAIPTRVGGRQPAHCAAVGKAMLAYREDSDIVDLA
                     TRKTRYSISTPAQLTAELAKVRAHGVAFDREESLPGFGCVAAPIGAVGEAVAAVSVCG
                     PMNRMMFDQRLAAPVRMTAMGIWRNVEDGPQRVAPTLQPVRPLLGALRAIPA"
     gene            complement(7803..9266)
                     /locus_tag="VFC01_15860"
     CDS             complement(7803..9266)
                     /locus_tag="VFC01_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN81141.1"
                     /translation="MDTDVHPKPADQIESWDFEADVVIAGYGIAGAAAAVEAASVGAD
                     VLVVERTGGWGGAAAMAGGFIYLGGGTRLQKACGFDDSVDNMAAFLNVAMGPGADENR
                     VGDYCAGSVDHFDWLVRCGVPFKPVFWGEPGWEPPGDEGLMFTGGENAFPFNIIAKPA
                     PRGHIPQMANKRAGEASAGYMLMKPLVDTATSLGVRAVYDVHASSLVVQSDGRVAGLI
                     ARQYGKPLAIRARRGVVLASGSFAYNDAMLAQYAPRLVGRPAASIEQHDGQAIRMAQA
                     LGADLAHMDATEVAFLVDPQQTVRGILVNGRGQRYVPEDMYSGRIGQLTLYQQDDTAY
                     LIIDGDAQEEAMAATSATPFLKRPASWICETVAELESEIGLPTGSLQATVNVYNDAAA
                     RGEDPLLHKKLEWLKPIGTPVGAIDLRASCAGFTLGGLQTTLDSEVLHVSGEAIPGLY
                     AAGRCTAGVAAWGYASGISLGDGSFYGRRAGRAAAKG"
     gene            complement(9270..10178)
                     /gene="bphC"
                     /locus_tag="VFC01_15865"
     CDS             complement(9270..10178)
                     /gene="bphC"
                     /locus_tag="VFC01_15865"
                     /EC_number="1.13.11.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009977156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biphenyl-2,3-diol 1,2-dioxygenase"
                     /protein_id="HZN81142.1"
                     /translation="MSVIKSLGYITVAASDIDRWRQFAFGVLGFAEGKGSNSSALYLR
                     MDERAARIIVVPGDVDRVLTVGWEVRDHAALEEVKVVLDAAGVAVKELSAAEADERRV
                     EEVITFEDPAGTTLEVFHGAVLDHSPVVTPFGARFVTGDQGLGHVVVPAVDANGLFDF
                     YTGVLGFRSRGAFRVPAPPEFGPIRVRFLGINERHHSLAICPAMNQDKPGLVHVMVEV
                     DSLDAVGQALDRVNAEGFQLSSTLGRHTNDKMVSFYVRAPGDWDIEFGTDGMRVDERY
                     YTAEEITADSYWGHQWVGEPPAAMRI"
     gene            complement(10181..11359)
                     /locus_tag="VFC01_15870"
     CDS             complement(10181..11359)
                     /locus_tag="VFC01_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN81143.1"
                     /translation="MTERVIDRVMEMAGQLREQAAEAEKIGRLTDQTTKAMKAAGNIR
                     LLQPTNYNGYEVHPREFAETVMATASLDPAAGWINGVVGVHPYQLAYADPRVAEEVWG
                     NDVDTWVASPYAPQGVAKPVDGGYIFNGRWQFSSGTDQCEWIILGAMLGDADGKPLMP
                     PQMLHMILPRKDYEIVEDSWNVVGLRGTGSKDVIVKDAFVPTYRTMDANEVMDGTAQR
                     KAGMTSTLYLMPWSTMFPLGITSAVIGIAEGALAAHLDYQRDRVGAYGAVKDDPYVMY
                     AIGEAAADINAARQELLANVDRIYDIVDSGKEASFEDRAAGRRTQVRAAWRAVTAVDQ
                     IFARSGGNALRMDKPLQRFWRDAHAGLAHAIHIPGTPYHASALSSFGVDPEGPLRALI
                     "
     gene            complement(11453..11722)
                     /locus_tag="VFC01_15875"
                     /pseudo
     CDS             complement(11453..11722)
                     /locus_tag="VFC01_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005568572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
     gene            11884..13479
                     /locus_tag="VFC01_15880"
     CDS             11884..13479
                     /locus_tag="VFC01_15880"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN81144.1"
                     /translation="MTLDAVVVGAGFAGLYALHKLRSQGLSVRVFEAAPDVGGTWYFN
                     RYPGARCDVESVDYCYSFSDELQQEWNWTEKYATQAEILRYINWVTDKLDLRRDITFS
                     TRVTSAVLDEQTLRWTVSTDSGEVVAARFCIMATGPLSAAMTPKFDGLDTFAGDLYHT
                     AQWPHEGVDFTGKRVAVIGTGSSGIQSVPVIAQQTEALYVFQRTPNFSVPAGNKPLTA
                     ADVAEIKANYDERRRLSWRSGGGSPHLAHPKLTMEASPEERRQAFEKRWELGGVLFSK
                     TFSDQMIDPVANDEARKFYEEKVRAVIDDPEVADLLIPTDHPIGTKRICTDTNYFQTF
                     NEPHVKLVSVRRTPIESIDATGINTTDAHYDVDAIVLATGFDAMTGTLARIDIVGRAG
                     EKLRDDWTDGPRTYLGLGVDGFPNLFLVSGPGAPAVLANMVLHAEAHVNWIAECITYL
                     DEHGHTGIEATPDAVDNWIAECAQRADTTLFPKANSWYMGANVPGKPRVFMLFIGGFA
                     VYNDICAEVADAGYKGFNLIKAS"
     gene            13479..14282
                     /locus_tag="VFC01_15885"
     CDS             13479..14282
                     /locus_tag="VFC01_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZN81145.1"
                     /translation="MSGLLDKVAVITGAARGTGRTHCERFADEGADVIALDIPAAATD
                     LGVSAAEVEKRGRRCVTGLADVRDLDALAAAVGAGVAELGRLDVIVANAGIHTAGAAA
                     WELTEDAWRNTLDVNLTGVWHTVKAAVGHLGDDGGSIVIISSTNGLRGTANTAHYTSS
                     KHAVVGLARTLANELGPRRIRVNTVHPGAVATAMTLNEATFRRLRPDLESPTADDVTE
                     VLAARNLLPVPWVDPVDVSNAVVFLASDEARYITGTQLVVDAGLTQKTS"
     gene            14279..15109
                     /locus_tag="VFC01_15890"
     CDS             14279..15109
                     /locus_tag="VFC01_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZN81146.1"
                     /translation="MSGRLAGKVALITGAARGIGRAQAVRFAQEGADIIALDICGPVD
                     TVVVPPATPSDLQETVRLVADAGGRVVSEIVDVRDTEAVQAATDRGVDQLGGLDVVCA
                     TAGITSRAMAVDLSENAWQTMLDVNLTGVWRTCRATAPHLINRGAGSMILTSSIAGMR
                     GLVGVAHYTAAKHGVVGLMRSLANELAPHNIRVNTVHPTNVDTPMIQNDHVRGAFRPD
                     LERVTREQFGEAARCMNMLPIPWVEPIDVANACLFLASDEARYITAVALPVDAGSTQR
                     "
     gene            complement(15113..15973)
                     /locus_tag="VFC01_15895"
     CDS             complement(15113..15973)
                     /locus_tag="VFC01_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN81147.1"
                     /translation="MAVELTYDGTLREMSTDAGVLRYHEAGEGPPLLLLHGSGPGVTG
                     WRNFRGILATFAEHFRCLIVEFPGFGVSDDFGGHPMATAYGAVDTFVETLGLDVVDII
                     GNSMGGGVGINFAIRQPDKVRRLVTIGGIGTNIFTPGPSEGIRLLQEFTENPTRQRLV
                     DWLHSMVYDPALVTDELIEERWALATDPPTLESARRMYGKAAFAAMMTAMRNSDAPLP
                     WATMHKVQAPTLLTWGRDDRVSPLDMALLPMRTIPNAELHVFPNCGHWAMIEAKQAFE
                     STVRSFLLRE"
     gene            complement(16076..16375)
                     /locus_tag="VFC01_15900"
     CDS             complement(16076..16375)
                     /locus_tag="VFC01_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81148.1"
                     /translation="MKKFGFATVLAGGLTAAILGLAAPAGAAPTGPGTAQDTINQLQA
                     DGYNVIVNHIGSTPLDQATVVAVRPGQTYSRTDSGNPGDSLETTVTGKTVYVDVK"
     gene            16562..17620
                     /locus_tag="VFC01_15905"
     CDS             16562..17620
                     /locus_tag="VFC01_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HZN81149.1"
                     /translation="MVLTPAASATVHRLPAIPSTVHWGYFDPRLEPAITIASGDLVAI
                     ETLTHHAGDAPDLLMDAGITDVFDRVTDRGPGPHLLTGPIAVEGSRPGDVLQVDILEA
                     TPRLPYGSNLAAHWGFLYRENPVERVTIYQFDTAARLGRALFGYDWTTTPLADQPGTI
                     VDPDMDARQPALPGVVVPLRPHFGTMGVAPAAAERVSSVPPGDHGGNVDNWRVGSGGT
                     MYYPVQVPGGLLSVGDPHVSQGDGEVSGTALEASLNGLLRLTIRRDLPFTVPVLETDT
                     ELLIHGFGDTLDEAMKAAALRTHGILQSYLGLSRADAYSFMSVAVDFTVTQVVDQRQG
                     VHGRIDKRCFPRWTGIPA"
     gene            17617..18042
                     /locus_tag="VFC01_15910"
     CDS             17617..18042
                     /locus_tag="VFC01_15910"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN81150.1"
                     /translation="MSDNGIRSFTFTPADGVPPAVAPFAHATAAGPTLYVTGQMPTDV
                     TGALVGDYVAIQTDQVLRNLMRVTELCGGALDDVVSVRAYLLDWNDYDAFNAAYAAWF
                     PDRLPSRTCIGVTGLAVGALVEIDWVCWRSDGWGATIGS"
     gene            18094..18996
                     /locus_tag="VFC01_15915"
     CDS             18094..18996
                     /locus_tag="VFC01_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZN81151.1"
                     /translation="MSILYTLSAIGAAGIGVIWLALQNIRVVKQFERGVVYRFGRVQS
                     RVRPPGLTWLVPIADRLQKVNMQIITLPVPAQDGITRDNVTVRVDAVIYFNVEDPVRA
                     AVDVQDYMSAIGQVAQTSLRSIIGKSNLDDLLSNRERLNQGLELLIDSPALGWGIHID
                     RVEIKDVLLPDSMKRSIARQAEAERERRARVITADGELQASEKLSQAAEVMAVEPAAL
                     QLRLLQTVVEVAAEKNSTLILPFPVELLRFLERSTPERKQEAKDDKSTNAETPELTAP
                     KVPDDARGIALDGPLTERRSGQNA"
     gene            19028..19429
                     /locus_tag="VFC01_15920"
     CDS             19028..19429
                     /locus_tag="VFC01_15920"
                     /inference="COORDINATES: protein motif:HMM:NF044909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81152.1"
                     /translation="MPLDADAVGRWFDEYFDTYAACARGEREMSTLLSLYGVPMVLTT
                     DEGVVTLMTDDEAAAVVQSQIDGLRAQRYHHTDVLHSEVMVLNSTSALLRASLSRRNA
                     DAGEFDSPTITYLVTDDVAGLRIVLLAVHGQ"
     gene            complement(19431..19718)
                     /locus_tag="VFC01_15925"
     CDS             complement(19431..19718)
                     /locus_tag="VFC01_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HZN81153.1"
                     /translation="MTSGLDDTSLPRDNGELVFEAPWQARALAMAVALVEKLDLPWDA
                     FRHRLMTEIASDPQRPYYESWCAALESMVIDLELTTPGALDAAAPTEREPL"
     gene            complement(19715..>19876)
                     /locus_tag="VFC01_15930"
     CDS             complement(19715..>19876)
                     /locus_tag="VFC01_15930"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HZN81154.1"
                     /translation="TVIPDGVEIRVWDSSAEARYLVLPMRPDGTDEMTEEQLAGVVTR
                     DSMIGVQRL"
BASE COUNT         3460 a         6403 c         6491 g         3522 t
ORIGIN      
        1 gatctggttg tagcggaagt cccgcgcgcc gacgccaccg aaacgttcgt cggccgcgaa
       61 acccagcaac ccgaccgcgc cggctttcgc gaacacctcg cgcggaatga tgccgtctgc
      121 ttcccactcg gcgatgttgg gtgcgacttc cttggctgcg aaatcgccag ccagctcggc
      181 gaacgcgcgg tggtcggcgt cgtagtgcac ccgctgcatg accggagcgt aggcatgtgc
      241 gcggtgttgg cgacgacgca tgtcccgctc agtagacagg agcgcccgcg tcggcggcgg
      301 acgcttcgat actcggtagt cgtgacgact gacgccgaac tgctgatcca tttgcgccag
      361 gccgaccctg gcgtcctcgt ggcggtgctg gctcagctca ccggcgatgc gtcggtgatc
      421 gatcggttcg gaccggcgat ctcgtacgtt cccgacccgc ccgaacgtgc gggtgtaacc
      481 gacccggata ctgcggacgc gctcgtggcg gccgttatcg aggcattgga tgctccgcgc
      541 tttgcgggcg cgcccgcaac tgtcgaccca gacctcttca cgcgactact gccactggcg
      601 ctcggcagcg acgtcgacga cgagttcgtt ccgctgttgc tcgagcaggg tggcttccag
      661 ctctcgcagc cgacgctgcc gcgaaccgtg ccgattccgg aaacatttaa ggtggccatc
      721 gtcggcgctg ggcttgccgg catcatcgcc gcgctcgccg ccgccgatgc gggggtcgcg
      781 tacgagatct tcgaccgcaa caacgaagtc ggcggcacct ggttgaccac gacctatccg
      841 ggcatcggcg tcgatacacc cgcggcgtat tactcgctgt cgcgtgaagt gaacccggat
      901 tggtcgagct actacccgca gggcggtgag tatcagtcct acctggtggc gctggccgac
      961 aagtacggtc tgcgcgagca cacccggttc ggcaccgagg tcgaggcctt gtggtgggac
     1021 gaggaccgcg atcactggca gatccattcg gttgatcgcg atggcaatcg cgacgtcagc
     1081 ttcgcccgtg tggtgatcac cgccgccggc tacctcaacc gaccacgctt gccggacctg
     1141 cccggccgag acatattcgc gggcattagc attcactccg cactgtggga tgcctcgctg
     1201 gacctcacgg gcaagcgggt ggcgatcatc ggcgcgggct gcacggcggt gcagatcgtc
     1261 gacgcctgtg tcgatcaggt ggaccacctg acggtgtttc agcggcagcc gcattgggtc
     1321 gcgcctcgca agcggctttc cgatgatgtg cccgaacacc gtcggtacct cggccgccac
     1381 ctgccgcatt acgcgaagtg gaaccggctg aagtcgttct ggggcacggc ggacaataac
     1441 tatccggtca tcctgcggga cgcggaatgg gcgaagaccc atctgtcgat ctcaccggcc
     1501 aacgacgtgc tgctacagat gtgcctcgag tacatcgacc gaaccttcgg cgagggaacg
     1561 gaattggcga agaaggtgac tcccgatttc gcgccgtacg gcaagcggat cattcgcgat
     1621 ccgggtggct actacgcggc gctgacccgt aaccacgtcg acgtggaagc gagcgagccc
     1681 gccgaggtga acgccgatgg aatcgtgacg gcagacggca ggcagatcga cctcgacgtg
     1741 atcatctacg cgaccgggta tcacctggac tttttgtcca ccatcgacat tcgcggccgt
     1801 ggcgggcgca gcctgaccga cgaatggggg gacagccccc gcgcatatcg cggtggcacg
     1861 gtgccgggat tccccaatct gttcatcaac tcggcgccga actacagccc cgggcacggg
     1921 gcgggcgcta atttctccat ggaggtgatg gcgcactata tcgtcgagtg cctgcaactg
     1981 atggcgctgc ggggcgcgac cacgatggaa gtgacgcagc aggcgtacga cgagtacgtg
     2041 gatgggatcg acgaggcaat ggcgggcacc gtctggtgtc acacccccag tgcgcacacc
     2101 tactaccgct ccggttcggg ccgggtggtg gtggccaccc cataccggct ggttgacctc
     2161 tggcatcagc accgagcgcc aatcgaagag cacttcgagc tcctatgacg caattgctca
     2221 gcggcaagac ggctttggtc accggcagca gccgggggat tggtcgcgcg attgcgcagc
     2281 ggctggcggc cgagagtgcg acggtcgtgg tgacggcgcg atcctacgag ccgtcggcgt
     2341 cggtgcgagg aggggaggcg ctggcgatac ccggcacgat ccgcgaaaca gtcgagctga
     2401 tcgaggaggc cggcggaaag gcgtttggtg tcgccgccga tctcgaggac accgaacagc
     2461 gtgagggcct cgtcaaaaag gtgctcgacc gcacgggccg catcgacatc ctcgtcaaca
     2521 atgcgggatt cgccgactat gggctcgtcg aacacatgga gatggatacc ttcgaccgca
     2581 cggtggagca ctacttgagg acgccgttcg tgctcgccaa ggcggcggta ccgcatatgc
     2641 gcaagcaagg ggcgggctgg atcgtcaaca tcggatccgt taccggagtt gcaccgatgc
     2701 ggccgtaccg tgagtacaac aagacctcgg gcgatgtggt ctacgcgtcg atgaaggccg
     2761 ctctgcacag gttcacgcag ggggtggcgg ccgagttggt cgatgcgaac atcgcggtaa
     2821 actgtgtcgg tccatccacg gctattcgca cacctggtgc ggcccaactg atcccggaca
     2881 ctttcccgac ggaacccgtc gagtatctcg ccgagaccgt gctggcgatg tgcttccttc
     2941 cggcagccga acgcaccggg ctggtagcat tcagccttca ttacccgtgg tcgcagaagc
     3001 tgaccgttca cagcctggac ggcgcctcgg tgctgccgcc gttggaaccg ccggtgacgg
     3061 ccaacccgaa catcctgtcc gccgggatgt gacgtaaagc ccaaggaaca caaagggtgt
     3121 agagagcctg cggtgcctgg aaaccggtcc gcacacgcgc ctgcgcggac cggtctcttc
     3181 agggttaggt ggccgtctca gaggcagagg gcgctgcatc tgatgacacc ggcatgatgg
     3241 ggatcttctt catggcgctc gctcggggat cgggtgccgc cgacgcagcg gcgtgattgt
     3301 cggcacctcg ggtgtaggtc cagacgcaga agggctcacc ctcagcgaat ttgaagtctt
     3361 tgggcggcag gttgatgtcg agcttgcaac ctgggaaaaa gggagccgag atccgctgct
     3421 cgaacgtgca attcatccag gctcgcgcag gcggatacag gccttcatcg gtgtattttt
     3481 gcagcacctc gcagcggcgc atgttgagca ccacgtgatc tgggttgtgc acgatgtact
     3541 cgccgccgta gccgagggtg ccgtcgatac tgaggcaggc cagtttcagg aaatcgacca
     3601 cgtacacagg ctcgatcttc atcaccttgg ccagcttggg gtactggtag tcgatgatga
     3661 gctcgtaaag gccagggaga ccgagccaga cctcttcttc ggggatgatc ttcgtccact
     3721 ggtcgatgaa gtagttgtgg cccttcaggt attgatccca gtaggtcttg atcagccgca
     3781 tgagggcgtc cttcgtcaga tcctcggtct ggacatcgaa cggaatggct tggtattggg
     3841 cgttcttcca atcccagccc ggaggtgcga tgaaccggcc catcggcatc gcttggcgca
     3901 tcccggcccg agacacctcg gcggcaatgt ccatagcggc gttgatgccc actttctcgg
     3961 cgtagatctt cacgtgggcg tagggcacga agttcaacgt gtcctcgaac acccgggcca
     4021 gtccgagcag gaaattccgc gagaagtctt cgatattgcc gaccttcccc atgaagtcat
     4081 gggtgtagtc gtcatagttc agttcgctat tcatttcggg ctctccttga cacttccacg
     4141 gcggggttgt gtcagaatct gcatgtatgt cagaatctga cagctagatg ttagggtgct
     4201 gtcgaccgga agatggccaa catcccactg tgcgggatcc gttgcatagt caggcgtttc
     4261 atgaatggag cctcaggtga gcgcgccgag tagaccaact ttgaccgagc ggcgtgccga
     4321 cgagctccgg atgacgatcg cgctcaccgc ccgcgacatc ttcctcgccg atggatccac
     4381 ctcggcgact gtcgagcgga tctgcgaggc ggtggggata gcgccgcgta cctttcaccg
     4441 ccacttcccg gtcaaggaag acgttgtgat gccactattc cgtcggttcg gtggtctgag
     4501 cgtcgaagtc ctgcaagatg ctccgcgcac gagtgacacg gtcgagaccc tggtgaaggc
     4561 attctcgaca gaggttgcca agcggggcga actggagatc gaccgcaagt tcatgtcgct
     4621 catgatcacc gatccccagt accggcttcg ttggttggac tggggccaag acttcgccga
     4681 cccgatctcc gaattcctgg ccgaacgcgt cgatctgggt ggtgacccgt tcatccgcac
     4741 actgccagcc cagctgataa tccagatctg ccgccaggcc tacgttcatt gggtggcgca
     4801 gggcaacttc cgcagtttgg aatcagcgtt acgtgccggg atgctgatgg tcatccgggc
     4861 attaccacct ttgacgcccg aagccgtggc ggcagtcaag tcaggaccga ctcgggcatc
     4921 gcgcaaaagg tatggcacac gcggtcgcga acgatgacat cggggcattc gggatcgaac
     4981 cagcggtcca ccaccgccca gtggatcggg tgcatcaggt aagggcccat cagcccgtcg
     5041 acgtcggtga actcctgctc aaagacgtga gtccactcag acgtcccgac ggggttgtcg
     5101 acgcggctca gctgccaggc ggtaatggtg ttcacgtagc gcggcatcag tcgcagttcg
     5161 tcttcgaagc gagcgacgat gtgggcgtcg gtgccgggaa gcacgcgcag cagcagcgcg
     5221 cgatacacgg tgccggtgtg ggaaccgcga acagaagcgt cgcctgaata acaggcgccg
     5281 ttgacacgcg tgatcgcgga gtcggtcagt gtcaccgcga aatctgtttc aacggaccgc
     5341 cattggtctt cgctgtcgaa gcgcaggtgc accaggatgt cgccgccgtt gcgaacgccc
     5401 ggcagcgtag gcgcaacgag ctggtgtcgc gcccttgtgg cggcggcccc ggcgcgcgca
     5461 gcggcaagca ggcggtcgcg atcgacttcg gcgacgtcga gcatccgagt gacgctatac
     5521 atcacagagt cggtcgacgt cggccgctgc ggccgccacg ctgcgcgtcc gttcttccac
     5581 gagttcgccg ataccgtccc accacggccc gagggtgggg tccggccgac ctttccacgt
     5641 catctcccac cacgcctgcg cgctggtcag cgaccaggtg atggtcaccg tgttggattg
     5701 atcgtcgaac cagatcggtg ggctgaccaa gacgtcgcga agcgtcatgc cgcgtgcgcg
     5761 tgcgccgggg gcgtactcgg cgaggtagca gtcgacaaac ttgcgggcac acccaggccg
     5821 ggtgacgact ctgtcgatga cgtagacctc ggatccggtc acgttgcgag agtgcgcctc
     5881 aaagtcgtcg ccggcaatgc cgcgtcccga tacacgggag ctgtggtcag gagacgggac
     5941 aactcgcggc acgtctcgac gcgagcttgc tgcgccaggt ccagtgccgg cattgtcatg
     6001 aacccgtgta tgccgccgtc gtatcggcac tgcaccgtca gcacctcggc agcctccagc
     6061 gcgttgccga atgcaactgc ctcgtcccgc aacggatcgt ggccggcgat gacgacgacg
     6121 gcgggcggca gcccacgcaa gtctgcattc agcggtgacg cataggggtg ttcgcgatcg
     6181 gcggccgagg gcacgtactg atcccagtac cactgcagtg cggggcgcgg gttgtagaag
     6241 cccttgccga acatgcggta cgactcggtg tcgaagttcg gagcgatcat cggatacagg
     6301 agcagctgtg ccaccagcga cggtgctcca cgatcgcgcg ccatcagtgc tgtggcggcc
     6361 gccaggttgc cgcccgcgct gtcgccaccc acgacaacgc ggcccgcatc gccgccgagc
     6421 gcatccgcat tgcgggcagc ccactgggtt accgcgaaca tgtcctcggc ggccgccggc
     6481 caccggtatt ccggcgccag ccggtatgca accgaaacga caacggcggg aatcagattc
     6541 gccaagctac gacaaaggcc gtcgtggctg tcgagatcgc agaacacaaa gccgccgcca
     6601 tgggcgtaca ccagcgtcgg caccggaccc gacatcgacg ccgggcggta gatgcgtacc
     6661 gcgacatcgc ctgccgggcc gtgaacggac gcgtcacgga tctcgccgac ctcttcgggt
     6721 gtcgacgacg cgacgaaacg tgaccgaatt gatgcccggg cctccgcacc cgtcatcgtg
     6781 tgtacgggcg gaaatccggc gtcgagcgcc tcgatgattt ccgcgatctg cgggtccaaa
     6841 ctcatgccgg gattgcgcgg agcgcgccaa gcagcggacg cactggctgc agcgtcggcg
     6901 ccacccgctg cgggccgtct tcgacgttgc gccatatgcc catcgctgtc atgcgcacgg
     6961 gcgcggccag gcgctgatcg aacatcatcc ggttcatcgg accgcacacc gacaccgcgg
     7021 cgactgcttc gccaacggct ccgatcggtg ctgcgacgca accgaatccg ggcagtgact
     7081 cttcacgatc gaacgccacc ccgtgggccc gaaccttcgc gagttcggcg gtcagttgtg
     7141 ccggcgtgct gatcgagtac ctcgtcttgc gcgtggccag atcgacgatg tcagagtctt
     7201 ccctgtaggc cagcatcgcc ttgcccacgg ccgcgcaatg cgccggctgg cgtccgccga
     7261 cgcgggtggg gatcgccgcg gtcatccggt cgccgatctt ctccaggtac accacgtcgg
     7321 agccgtcgag cacggcgagg tgtacgacga gccccgtggc gcggtggaga tcgtgcagca
     7381 aggggatcgc cgcgcgatgc agccggtcct ggtgcaccgc gagcgatccg agttcaacga
     7441 gccgcatgcc cagctcgtag tcgcggccgc tccggcgcag ccaccgaagc tgaaccaacc
     7501 gttcgagcat gcgatgcgcc gaggaccggg gcaggcccgt gcggcgcacg atctgggcca
     7561 gcgtcagccg tccgggtccg tcgaaggcgt ccaacaccag cgacacccgg tcgatgacgg
     7621 cactcggagt ggtggtttcg acggtgacgg tcatgacgcc tcctcgcgat cgcatatgac
     7681 taataggaat atgactgttt gtaccatagg tctgtggctg ctgtcacata tgcatgcgaa
     7741 attgatccgg cccacagcgg gcgcgctgcg ttgaactgcg taaatgcggt gggaggaggt
     7801 tgtcagcctt tggctgcggc gcggccggca cggcggccgt agaaactgcc gtcgccgagg
     7861 gagatgccgc tggcgtagcc ccacgccgcg acgcccgctg tgcagcgccc cgccgcatac
     7921 aggccgggga tcgcctcgcc actgacgtgc agcacctctg agtccagagt ggtctggagc
     7981 ccgcccagtg tgaagccagc gcagcttgcc cgcagatcga tcgcgccgac gggcgtcccg
     8041 atcggcttca accattcaag tttcttgtgc agcaacggat cttcgccgcg cgccgcggcg
     8101 tcgttgtaca cgttgaccgt ggcttgcagc gatccagtag gtagtccgat ctcggattcg
     8161 agttcggcga ctgtctcgca gatccacgag gctgggcgtt tgaggaacgg tgtcgcggag
     8221 gtcgcggcca tcgcctcctc ctgcgcatcg ccatcgatga tcaggtaggc ggtgtcgtct
     8281 tgttggtaga gcgtgagctg cccgatgcga ccggagtaca tgtcctcggg gacatagcgc
     8341 tgtccgcgcc cgttgaccag gatgccgcgg acggtctgct gtggatcgac taggaacgcc
     8401 acctcggtgg cgtccatgtg ggccaggtcg gcgccgagtg cctgggccat ccggatcgcc
     8461 tgcccgtcat gctgttcgat tgacgcggcg ggacgcccga cgagccgcgg tgcgtactgc
     8521 gcgagcattg cgtcgttgta cgcgaagctg cccgaggcaa ggacaacgcc ccgtcgcgcg
     8581 cgaatggcga gcggcttacc gtactggcgg gcgatgagcc cagcgacacg gccgtcggac
     8641 tgcaccacca acgacgatgc gtggacgtcg tacaccgccc gaacgcctaa tgacgtggcg
     8701 gtgtcgacca gaggcttcat cagcatgtag ccggcgctgg cttcgcctgc ccgtttgttg
     8761 gccatctgcg gaatatggcc gcgcggagcg ggtttcgcga tgatgttgaa cggaaacgcg
     8821 ttctcgccgc cagtgaacat cagaccttcg tccccgggcg gctcccaacc gggctcgccc
     8881 cagaacaccg gcttgaaggg cacaccgcac cgcaccagcc agtcgaaatg gtcaacgctg
     8941 ccggcgcagt agtcgccaac ccggttctcg tcggcgcccg ggcccatcgc cacattcagg
     9001 aatgccgcca tgttgtcgac ggaatcgtcg aagccgcagg ccttttgcag cctagtgccg
     9061 ccgcccaggt agatgaaccc acccgccatc gcggcggccc caccccagcc tcctgtgcgt
     9121 tcgacgacga ggacgtcggc accgaccgag gcggcctcga ccgctgcggc ggcaccggcg
     9181 atgccgtatc ccgcgattac gacatcggct tcgaagtccc aactctcgat ctgatctgcc
     9241 ggcttgggat ggacgtcggt atccatcggc tagatccgca tcgccgcggg cggctcgccg
     9301 acccactggt gaccccagta gctgtcagcg gtgatttcct ctgccgtgta atatctttcg
     9361 tcgacgcgca tgccgtcggt gccgaactcg atatcccaat cgccgggtgc gcggacgtag
     9421 aacgagacca ttttgtcgtt ggtgtggcgg cccagcgtcg aggacagctg gaagccctcc
     9481 gcgttcaccc ggtccagcgc ttggccgacc gcgtcgaggc tgtcgacttc gaccatgacg
     9541 tggacgagac cgggcttgtc ctggttcatt gcgggacaga tggcgaggct gtggtggcgc
     9601 tcgttgatgc cgaggaaccg gacgcggatc ggaccgaact ccggcggggc gggcactcgg
     9661 aatgcgccgc gcgagcggaa acccaagacg ccggtgtaaa agtcgaacag gccgttcgcg
     9721 tcgaccgcgg gcaccaccac atggccgagc ccctgatcgc cggtgacgaa ccgcgccccg
     9781 aagggcgtca cgaccgggct gtgatcgaga accgcgccgt ggaagacctc gagcgtcgta
     9841 cccgcggggt cctcgaacgt gatgacttcc tcgacccggc gttcgtccgc ctcggcggcc
     9901 gacagttcct tgaccgcaac accggcagcg tccaagacga ccttgacttc ctccagcgcg
     9961 gcgtggtcgc gcacctccca gccgacggtc aatacccggt cgacgtcgcc ggggacgacg
    10021 atgatgcgtg ccgcgcgctc gtccatccgt aggtacagcg ctgacgagtt ggaacccttg
    10081 ccctcggcga atcccaggac gccgaaggcg aactggcgcc accggtcgat gtcgctggcc
    10141 gcaacggtga tgtatcccaa gctcttgatg acgctcatct ctagatcagc gcccgcaggg
    10201 gaccttcggg atcgaccccg aatgagctca aagcggacgc gtggtacggg gtgccgggaa
    10261 tgtggatcgc gtgagcgaga ccggcgtgcg cgtcgcgcca gaagcgttgc agcggtttat
    10321 ccattcgcaa ggcgttgcct cccgagcgcg cgaagatctg gtcgaccgcc gtgacagcgc
    10381 gccacgccgc acgcacctgc gtccggcggc ccgcggcgcg gtcctcgaac gaggcttcct
    10441 tgccggagtc gacgatgtcg tagatgcggt cgacattggc cagcagttcc tggcgggcgg
    10501 cgttgatgtc tgcggcggcc tcaccgatcg cgtacatgac gtaggggtcg tccttgaccg
    10561 cgccgtaggc gccgacgcgg tcgcgctggt aatccaggtg ggcggcgagt gctccttcag
    10621 cgatgccaat caccgcagac gtgatcccca gcgggaacat cgttgaccac ggcatcaggt
    10681 acagcgtcga ggtcatgccg gccttgcgct gcgcggtgcc gtccatcacc tcgttggcat
    10741 ccatcgtgcg gtaggtgggc acgaacgcgt ctttgacgat gacgtccttc gacccggttc
    10801 cgcgcagccc gaccacattc cacgagtctt cgacgatctc gtaatccttg cgcggcagaa
    10861 tcatgtgcag catctgcggc ggcatcaacg gctttccgtc ggcgtcgccg agcattgcgc
    10921 cgaggatgat ccactcgcac tggtcggtgc ccgagctgaa ctgccaccgg ccgttgaaga
    10981 tgtagccgcc gtcgacgggc ttggccaccc cctgcggtgc gtacggcgaa gcgacccagg
    11041 tgtcgacgtc gttgccccag acctcctcgg ccacgcgggg atccgcgtac gccaactggt
    11101 acgggtgcac gcccacgaca ccgttgatcc atcccgcagc ggggtcgagc gacgccgtgg
    11161 ccatcacggt ttctgcgaac tcgcgagggt ggacttcgta gccgttgtaa ttggtgggct
    11221 gcagcagcct gatgttgccc gctgctttca tcgccttggt ggtctggtca gtcaaccggc
    11281 cgatcttctc cgcctcggcg gcctgctcgc gcagttggcc ggccatctcc atcacccggt
    11341 cgattacccg ctcggtcatc gtgtcgtcct tactgtgggg gcgtgattca tggtctaccg
    11401 caaccggcgt tggcgccgca gccaatcccg gtcagcggaa ccgagtcggc ggtcaaaact
    11461 cgatctgcac gtcgtcggag acgggtcggg cctggcaggc caggatgagg ccggcctgac
    11521 ggtcctcggg ttcgagaacg tcgctcggag ccatgtcgac ctcgccggaa acgacggtgc
    11581 acacgcacga gccgcagtgg ccttcgcggc acgagtgggg cacatcgatg cccgcggcga
    11641 gcatcacgtc gacgagcgtg gattgccgcg gccaacggac ttgatgtcga tgtccgtcga
    11701 gctccacctc agcggtggcg gctgcttcca gcgtgtcgtc agctatagac atgtccgaca
    11761 ttgtgggggc gcgattgtcg gcggcagccg tgggcgcccg atgttcggga gggaccaaca
    11821 cgagcgtccg gtcaccggga agcgacccgc ggtggttagc gcacaccccc tacggtcccg
    11881 atcgtgaccc ttgatgccgt tgttgtcggt gccggtttcg cgggcttgta cgcgttgcac
    11941 aagttgcgct cgcaagggct ctcggtgcgg gtgttcgagg cggcacccga cgtgggtggt
    12001 acctggtact tcaaccgcta tcccggtgct cgctgcgatg tcgaaagcgt cgactactgc
    12061 tactccttct ccgacgaact gcagcaggag tggaactgga ccgagaagta cgcgactcag
    12121 gccgaaatac tgcgctacat caactgggtt accgacaaac tcgacctgcg tcgcgacatc
    12181 accttcagca cacgggttac gtctgcggtc ctcgacgagc aaacgctgcg ctggacggtg
    12241 agcaccgata gcggtgaggt cgtcgccgcc cgcttctgca tcatggccac cggcccgctg
    12301 tcggcggcca tgacaccgaa gttcgacggc ctggacacgt tcgcaggcga cctctaccac
    12361 accgcgcagt ggccgcatga aggcgtcgac ttcaccggca aacgggtcgc cgtcatcggc
    12421 acggggtcct cgggcattca gtcggttccc gtgatcgccc aacagaccga ggcgctctac
    12481 gtcttccagc gcacaccgaa cttcagcgtc ccggccggga acaagccgct gaccgccgcc
    12541 gacgtcgccg agatcaaggc gaactacgac gagcggcgcc gactctcgtg gcgcagcggc
    12601 ggcggctcac cgcatctcgc tcaccccaag ctgacgatgg aggcttctcc cgaggagcgg
    12661 cgtcaggcgt tcgagaagcg ttgggagctg ggcggtgtgc tgttctcgaa gactttctcc
    12721 gaccagatga tcgacccggt cgccaacgat gaggccagga agttttacga ggagaaggtc
    12781 cgcgccgtca tcgacgaccc ggaggtcgcc gatctgttga tccccaccga tcatccgatc
    12841 ggcaccaaac gtatctgcac cgacaccaac tactttcaga ccttcaacga gccccatgtg
    12901 aaactcgtca gcgtgcgcag gacgcccatc gagtcgatcg acgcgaccgg aatcaacacc
    12961 accgacgcgc actatgacgt cgacgcgatc gtgctcgcga cgggcttcga tgcgatgacc
    13021 gggacgctgg cgagaatcga catcgtcggc cgggccggtg aaaagctgcg tgacgattgg
    13081 accgatggtc cgcggaccta cctggggctt ggggtggacg ggttccccaa tttgttcctg
    13141 gtgtccggac cgggcgcccc agcggtgttg gccaacatgg tgttgcacgc cgaagcacat
    13201 gtgaactgga tcgccgagtg catcacctac cttgacgagc acggccacac cgggatcgag
    13261 gccactccgg atgcggtgga caattggatc gccgaatgcg cccagcgcgc tgacaccacc
    13321 ctgttcccca aggcgaattc ctggtacatg ggggccaacg tgccgggcaa gccgcgggtg
    13381 ttcatgttgt tcatcggcgg tttcgcggtc tacaacgaca tctgtgcaga ggtcgccgac
    13441 gccggttaca agggtttcaa cctgatcaag gcgtcgtgat gtccggattg ctcgacaagg
    13501 tcgccgttat cacaggagcc gcacggggca ccggccgcac ccattgtgag cggttcgccg
    13561 atgagggcgc ggacgttatc gcgctcgaca tccccgcggc agcaaccgat ctgggggtca
    13621 gcgcggcgga ggtcgagaaa cgtggacgac gttgcgtaac gggcttggca gatgtgcgcg
    13681 acctcgatgc gctggcggcg gcggtcggtg ccggcgtcgc tgaacttggg cggctggacg
    13741 tcatcgttgc caatgccggc atccacaccg cgggcgctgc ggcctgggag ctgaccgagg
    13801 acgcgtggcg aaacacgctc gacgtcaacc tcaccggagt gtggcacacc gtcaaggcgg
    13861 ccgtcggcca tctcggagac gacggcgggt cgatcgtgat catcagttcc accaacggat
    13921 tacgcggcac ggccaacacc gcgcattaca cgtcgagcaa gcatgcggtg gtcggcctgg
    13981 cccgtacgct ggccaacgaa ctcggcccga ggcgcatccg ggtcaacacc gtccatcctg
    14041 gtgcggtcgc caccgcgatg acgctcaacg aagccacgtt caggcggtta cgacccgatc
    14101 tggagagccc gaccgccgat gatgtcacgg aggttctggc cgcaagaaac ctgctccctg
    14161 tgccatgggt ggaccccgtc gacgtcagta acgccgtcgt gttcttggca tccgacgagg
    14221 cccgctacat cacgggtact cagcttgtcg tcgacgccgg cctgacccag aagacttcat
    14281 gagcggtcgg ctagccggca aggtcgcctt gatcaccggt gcggcaaggg gaatcgggcg
    14341 tgcgcaggca gtgcgtttcg cgcaggaggg cgccgacatc atcgccctcg acatctgcgg
    14401 tccggtggac accgtcgtcg tcccgcccgc cacgccgtcc gatctccagg agacggtcag
    14461 gttggtcgcc gacgcgggtg gccgcgttgt cagcgagatc gtcgacgtcc gcgacacaga
    14521 agcagtgcag gccgcgaccg accgcggtgt cgaccagctt ggcggtctcg acgttgtctg
    14581 cgcaacagca gggattacgt cgcgagcgat ggctgtcgat ctcagtgaga acgcctggca
    14641 gacaatgctg gacgtcaacc tgaccggcgt atggcggacg tgccgcgcaa ctgcgccgca
    14701 cctcataaat cgcggtgcgg ggtcgatgat tctgaccagc tcaatcgcgg gcatgcgtgg
    14761 cttggtcggg gtggcccatt acaccgcggc caagcacggt gtcgtcgggc tgatgcgtag
    14821 cttggccaac gagcttgcac cgcacaacat tcgggtcaac acggtgcacc ccaccaatgt
    14881 cgacaccccg atgatccaga acgaccatgt ccgcggtgcg ttccggcccg acctcgagcg
    14941 ggtgacccgt gagcagttcg gcgaggcggc gcgttgcatg aacatgttgc ccatcccgtg
    15001 ggtcgagcca atcgatgtcg ccaacgcctg tctgttcctc gcctcggacg aggcccgtta
    15061 catcacggcc gtcgcgctgc cggtcgacgc gggaagcacc cagcgctgaa tcctattcgc
    15121 gcagcaagaa agaccgaact gtcgactcga acgcctgctt ggcctcgatc atggcccagt
    15181 gcccgcagtt ggggaacacg tgcagctcgg cgttgggaat ggtgcgcatc ggtaacaacg
    15241 ccatgtcgag cgggctgacg cgatcgtcac ggccccatgt caagagcgtc ggtgcctgaa
    15301 ctttgtgcat ggtggcccag ggcagtggcg cgtcggagtt gcgcatcgcg gtcatcatgg
    15361 cggcaaaagc ggccttgccg tacatgcggc gtgcgctctc cagcgttggg ggatctgtcg
    15421 ccaatgccca gcgttcctcg atcagctcat cggtgaccag cgccgggtcg tagaccattg
    15481 agtgcagcca gtcgaccagc cgctggcgag tcgggttctc ggtgaactcc tgcaggagcc
    15541 tgatgccttc gctggggccg ggcgtgaaga tgttcgtccc gatcccgccg atcgtgacaa
    15601 gacggcgaac cttgtccggc tgacggattg cgaaattgat gccgacaccg ccacccatcg
    15661 aattgccgat gatgtcaacg acatctaggc cgagcgtctc gacaaaggtg tccacggcac
    15721 cgtaagcggt cgccatcggg tgaccgccaa agtcatcact gacgccgaaa ccggggaatt
    15781 ccacaatcag gcaacggaaa tgctcggcga atgtggcaag aattccgcgg aagttccgcc
    15841 agccggtgac gccggggccg gagccgtgca gaagcagcag gggagggccc tcgcctgctt
    15901 cgtggtagcg caggacaccg gcgtcagtag acatttcacg aagcgtgccg tcgtaggtca
    15961 actccactgc catagcttgg catcgtggca cagagcttcg ggtggcccgt tccgatcagc
    16021 gggcgaaatg acgaaggggc accccttgtg ggggtgcccc ttcgctttgg gcgggttact
    16081 tgacgtcgac gtagaccgtc ttaccggtga cagtggtttc gaggctgtca cccgggttgc
    16141 ccgaatcggt ccggctgtag gtttggccgg gccgaactgc gaccacggtg gcttggtcca
    16201 acggggtcga accgatgtgg ttgacgatca cgttgtagcc gtcggcctgc agttggttga
    16261 tggtgtcctg ggcggtgccc ggaccggtcg gagcggcgcc cgccggtgcg gccaggccaa
    16321 ggatcgcggc ggtcaatccg ccggcaagta cagtcgcgaa tccgaacttc ttcatttctc
    16381 ggtgcctctt cttttcgtct tcttctgttg tgtcgattcg ctgcagctgc cttgataaac
    16441 gaccaagatc accgctttaa ttccattggc aggatttggt ccaccgttgg cagaaataaa
    16501 tctgttgacg cgtgtgtgtg ctcgggagga cgccgcacga cccgactggc atgatcaccg
    16561 gatggtgctc acgcccgccg catccgcgac cgtccaccgg ctgccggcca tcccgtctac
    16621 cgtgcactgg gggtacttcg atccacgact cgagccggca atcaccattg cctcgggcga
    16681 tttggtggcc atcgagacgc tgacccacca cgccggcgat gcacccgatc tgctgatgga
    16741 tgcgggcatc acggacgttt tcgaccgcgt caccgaccgg gggcctggcc cgcatctgct
    16801 gaccggcccg atcgccgtcg agggctcccg tcctggcgat gtgctgcagg tcgacatcct
    16861 cgaagccacg ccgcggctgc cgtacggctc caacctcgct gctcattggg gctttctgta
    16921 ccgcgagaac ccggtcgaac gcgtgaccat ctaccagttc gacaccgcag cccgcctggg
    16981 ccgggcgctg ttcgggtacg actggactac gacgccgctg gccgaccagc ccggcacgat
    17041 cgtcgaccct gacatggacg cccgccagcc tgcgctgccc ggcgtcgtcg tgcccttgcg
    17101 gccgcacttc ggcaccatgg gtgtcgctcc ggccgcagct gagcgcgtgt cgtcggtgcc
    17161 acccggtgac catggcggca acgtcgacaa ttggcgcgtc gggtcaggcg ggacgatgta
    17221 ctacccggtg caggtgcctg gcggcctgtt gtccgtcggc gacccccacg tctcgcaggg
    17281 cgatggcgaa gtcagtggca ccgcactgga ggcatcactg aatggcctgt tgcgcttgac
    17341 cattcgccgt gatctgccgt tcaccgtgcc cgtcctggag accgacaccg aattgctgat
    17401 ccacggtttc ggcgacacgc tcgacgaagc gatgaaggcc gcggcgctac gcacgcacgg
    17461 cattctgcag agttaccttg ggctttcgcg cgcggatgcc tactcgttca tgtccgtcgc
    17521 cgtcgacttc accgtcacgc aggtagtcga ccagcggcag ggtgtgcatg gccgaatcga
    17581 caagcgctgc ttcccccgct ggaccgggat accggcatga gcgacaacgg gattcgctct
    17641 ttcaccttca ctcccgccga cggtgtgccg ccagccgtcg caccgttcgc ccatgccacc
    17701 gcggccgggc cgacgttgta cgtcaccggc cagatgccga ctgacgtcac cggcgcgctg
    17761 gtcggcgatt acgtcgcaat ccaaaccgat caggtgctcc gcaacctgat gcgcgtcacc
    17821 gagttatgcg gcggggcact ggacgacgtc gtctccgtcc gcgcctacct gctcgactgg
    17881 aacgactacg acgccttcaa cgccgcctac gccgcctggt ttcccgaccg gctgccgtcg
    17941 cgcacgtgta tcggtgtcac cggactcgcg gtcggtgctc tcgtcgagat cgattgggtg
    18001 tgctggcgaa gcgacggctg gggggccaca atcgggtcat agggcccgcg aggccaaagg
    18061 accgccgcgg gttggtccgt cgaggaggac accatgagca tcctctacac actctccgcg
    18121 atcggcgccg ccgggatcgg cgtgatctgg ctggcactgc agaacatcag ggtggtcaag
    18181 cagttcgaac ggggcgtcgt ctaccgcttc ggccgggtgc agagccgcgt ccggccgccc
    18241 ggcctgacgt ggctggtccc gatcgctgat cgcctgcaga aggtgaacat gcagatcatc
    18301 acgttgccgg tgcccgccca agacggcatc acgcgcgaca acgtgaccgt gcgcgtcgac
    18361 gccgtcatct acttcaacgt cgaggacccc gtccgcgcgg ccgtcgacgt gcaggattac
    18421 atgtccgcga tcgggcaggt ggcgcagacc tcgctgcggt cgatcatcgg caagagcaat
    18481 ctcgacgacc tgttatccaa ccgcgagcgg ctcaaccagg gtctcgagct gctgatcgac
    18541 agtcccgcgc tgggctgggg catccacatc gaccgggtcg agatcaagga cgtcctgctg
    18601 ccggactcga tgaagcggtc gatcgcccgc caggccgagg cggagcggga gcgacgggcg
    18661 cgcgtcatca ccgccgacgg cgagctgcag gcgtccgaga agctgtccca ggccgccgag
    18721 gtgatggcgg tggagccggc ggcgctgcag ctgcggctgc tgcagaccgt ggtcgaggtc
    18781 gccgcagaaa agaactccac gctgatcctg ccgttcccgg tcgagctgct gcgattcctg
    18841 gaacgctcga cgccggaacg caaacaggag gcgaaggacg acaagtcaac caacgccgag
    18901 acgccagaac tgactgcgcc aaaggttcca gacgacgccc ggggcatcgc actcgacgga
    18961 ccgctgaccg aacgccggtc cggccagaac gcttagcacc ggcaaacctt tcgggcatga
    19021 tcagctcatg cctcttgacg ccgacgcggt tggtcgctgg ttcgacgagt acttcgacac
    19081 gtacgcggcg tgtgcccgag gcgagcggga aatgtccacg ttgctgagtc tctacggggt
    19141 tccgatggtc ctcaccaccg atgaaggtgt ggtcacgctc atgacggacg acgaagccgc
    19201 ggccgtggtg cagagccaaa tcgacggcct gcgggctcag cgctatcacc acacggacgt
    19261 cctgcattcc gaagtgatgg tcctcaattc gacgtctgcg ctgctgcggg cttcgttgtc
    19321 gcgccgcaac gccgacgctg gcgagttcga cagcccgacg atcacgtacc tggtaactga
    19381 cgacgtcgcc gggttacgga tcgtgttgct agctgtgcac ggtcagtgac ttagagcggt
    19441 tcgcgctcgg tcggcgcggc ggcatcgagt gcgccgggcg tcgtgagttc caggtcgatg
    19501 accatggatt ccagcgctgc gcaccaactt tcgtagtaag ggcgctgcgg gtcactggca
    19561 atctccgtca tcagtctgtg acggaatgcg tcccacggta ggtcgagttt ctccacgagt
    19621 gcgacggcca tcgccaaagc gcgcgcttgc cacggcgctt cgaaaacgag ctcgccgttg
    19681 tcgcggggca gcgaggtgtc gtcgagtccg ctcgtcaaag ccgctgcacc ccgatcatcg
    19741 agtcacgggt gacgacgccg gcgagctgct cctccgtcat ctcgtccgtc ccgtccgggc
    19801 gcatcgggag caccagatag cgcgcctcgg cgctggaatc ccacacccgg atctcgacgc
    19861 cgtcggggat gaccgt
//
LOCUS       DASRJA010000358         4783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1462865,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000358 DASRJA010000000
VERSION     DASRJA010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462865"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFC01_15935"
     CDS             complement(<1..561)
                     /locus_tag="VFC01_15935"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HZN81155.1"
                     /translation="MNEPSQWGTTAWAQLVAVVALIAFLHVPLGNYMARVYATGPDWR
                     VEGAIYRVVGVQPDDHQRWTKYLSSLLAFSAVSLLFLYALLLLQVNLPEPWGHKGMTP
                     ALAFNTAISFTTNTSWQNYPGESTLGHVGLAAGLGVQAFASCAVGMCVAVALIRGLAQ
                     YQNEELGNFWVDLVRSVVRVLLPLSIV"
     gene            964..1053
                     /gene="kdpF"
                     /locus_tag="VFC01_15940"
     CDS             964..1053
                     /gene="kdpF"
                     /locus_tag="VFC01_15940"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020108199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HZN81156.1"
                     /translation="MSYENVVGLVLSILLALFLFAALLFPERF"
     gene            1053..2723
                     /gene="kdpA"
                     /locus_tag="VFC01_15945"
     CDS             1053..2723
                     /gene="kdpA"
                     /locus_tag="VFC01_15945"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HZN81157.1"
                     /translation="MSTTTAGIMFLVALIVALAAVHVPLGDYMYRVYSSTKDSRAERA
                     IYRLIGADPKAEQTWGAYARSVLAFSAISILFLFIFQLVQGKLPLHLNDPATQMTPAL
                     AWNTAVSFVTNTNWQAYSGESTQGHLVQMAGLAVQNFVSAAVGMAVAIALVRGLARRN
                     TGELGNFWVDLVRGTIRILLPISVLFAIILVIGGSIQNFHLHDQVVNTLAGGQQTITG
                     GPVASQEAIKELGTNGGGFYNANSAHPFENPNSWTNWVEIFLLLVISFSLPRTFGRMV
                     ESKKQGYAIAAVMAVIATISVSFMMLFQLQHHGTVPTAVGAAMEGVEQRFGVANSAVF
                     ADATTLTSTGAVNSFHDSYTSLGGMITLFNMQLGEVAPGGTGSGLYGMLILAIITVFV
                     AGLMVGRTPEYLGKKITPREIKLAATYFLITPLIVLTGTAVAMALPGQRASMLNTGPH
                     GLSEVLYAFTSAANNNGSAFAGISVNTEWYNTALGLAMAFGRFLPMIFVLALASSLAK
                     QGKAPESIGTLPTHRPQFVGMVAGVTLILVALTFLPMLALGPLAEGIH"
     gene            2723..>4783
                     /gene="kdpB"
                     /locus_tag="VFC01_15950"
     CDS             2723..>4783
                     /gene="kdpB"
                     /locus_tag="VFC01_15950"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HZN81158.1"
                     /translation="MSTPTIHSTPSQAKAGTPKRVQGGLLDPKMLWKSLPDALRKLDP
                     RTLWRNPVMFIVEIGAVWSTILAIADPSWFGWLTVIWLWLTVVFANVAEAVAEGRGKA
                     QAESLRRTKTATMARRLVDWTPGAPGREEEVAAPLLQQGDIVVVEAGQTIPGDGDVVE
                     GIASVDESAITGESAPVIRESGGDRSAVTGGTTVLSDRIVVKITQKPGESFIDRMIAL
                     VEGANRQKTPNEIALNILLASLTIIFVFAVATLQPLAIFSKANNPGVPDSLSLTGNGV
                     SGIVMVSLLVCLIPTTIGALLSAIGIAGMDRLVQRNVLAMSGRAVEAAGDVNTLLLDK
                     TGTITLGNRQAAAFVPVNGVSPEVLADAAQLSSLADETPEGRSIVVFAKQEYGLRART
                     PGELDKAHWVEFSAQTRMSGVDLENGHRLRKGAASSVADWVRGEGGRVSTELGDIVDG
                     ISAAGGTPLVVGEVIEGQARVLGVIHLKDVVKQGMRERFDEMRRMGIRTVMITGDNPL
                     TAKAIADEAGVDDFLAEATPEDKMALIKKEQAGGKLVAMTGDGTNDAPALAQADVGVA
                     MNTGTSAAKEAGNMVDLDSDPTKLIEIVEIGKQLLITRGALTTFSIANDIAKYFAIIP
                     AMFVALFPGLDLLNIMRLHSPQSAILSAVIFNALVIIALIPLALRGVRYKPSSASQLL
                     SRNLL"
BASE COUNT          881 a         1579 c         1459 g          864 t
ORIGIN      
        1 gacgatcgaa agtggcagca ggacacgcac gacggagcgc acgaggtcca cccagaagtt
       61 gccaagctcc tcgttctggt actgcgcaag cccccgaatt agcgcgacgg cgacacacat
      121 tcccaccgcg caactagcga acgcctgcac gccgaggccg gccgccaaac caacgtggcc
      181 gagcgtcgat tcaccgggat aattctgcca actcgtattt gtcgtaaagg aaattgccgt
      241 attgaacgct aaggccggtg tcatcccttt atggccccac ggctccggca aatttacttg
      301 caaaagcaaa agggcgtaga gaaacagcag gcttaccgcc gaaaaggcca gcagcgaact
      361 cagatacttc gtccatctct ggtggtcgtc gggctgcacc cccacgaccc ggtagatggc
      421 cccctccacc cgccagtcgg gtcccgttgc atacacccgc gccatgtaat tacccagcgg
      481 gacgtgcaga aacgcgatca gggcaacgac cgcgacgagc tgcgcccagg cggttgttcc
      541 ccactgcgac ggttcgttaa tggctccgac tcccttaggc ggcgataatt ccgcgccaac
      601 gctacgagtt accctccgat taatcggccg ttagttacgc aatttctacg ccgcacctgc
      661 accaattgac ggcatcttga tgtcgcctgc gtcacccctg aaaagcagcc gcggcggaaa
      721 accgtaaagg tccgtgaccc gggcgtaaag aaagcgtcaa cgctcgttac cgacacagcc
      781 tgccaggtct agcttcgtgt tcggccctta acgccgaggg ccaggcgatg gctgctcgag
      841 cgacacccga gcgccctcag gtttccgtac tgatcggagt gacatgtccg ttgtggtgta
      901 catcgtgctg acggtggtga tcttcgccgt cctcggcctg gttcagaagt tggtcgaggg
      961 gctgtgagct acgaaaacgt cgtcggcctt gtgctgtcga ttctccttgc gctgtttctc
     1021 ttcgccgccc tgctcttccc ggagaggttc tagtgagtac tacaaccgcg gggatcatgt
     1081 tcctcgtcgc gctaatcgtc gcgctggcgg ccgtgcatgt gccgctgggc gactacatgt
     1141 accgcgtcta ctcgtctacg aaggactcgc gagccgaacg cgccatctac cggctcatcg
     1201 gtgccgaccc gaaagcggaa cagacctggg gcgcctacgc ccgcagcgtg ctggccttct
     1261 ctgcgatcag cattctgttc ctgttcatct tccaactcgt ccagggcaag cttccgctgc
     1321 acctgaacga tcccgccacc cagatgactc cggcgctggc gtggaacacc gcggtcagct
     1381 tcgtgacgaa caccaactgg caggcctact ccggtgaatc cacccagggt cacctcgtgc
     1441 agatggccgg actcgccgtg cagaacttcg tctccgcagc ggtcggcatg gccgtcgcga
     1501 tcgccctggt tcgcgggctg gcccggcgca acaccggcga gctcggcaac ttctgggtgg
     1561 acctggtccg cggcaccata cgaatcctgc tgccgatctc ggtgctcttc gcgatcatcc
     1621 tggtcatcgg cggatccatc cagaacttcc acctgcacga ccaggtcgta aacacgctgg
     1681 ccggcggcca gcagaccatc accggtggac cggtggccag ccaggaagcc atcaaggagc
     1741 tcggcaccaa cggcggtggc ttctacaacg ccaactccgc ccacccgttc gagaacccga
     1801 actcgtggac gaactgggtt gagatcttcc tgctgctcgt catcagtttc tcgctgcccc
     1861 gcaccttcgg ccgcatggtc gagagcaaga agcagggcta cgcgatcgct gccgtcatgg
     1921 ccgtcatcgc cacgatcagc gtcagcttca tgatgctgtt ccagctgcag catcacggca
     1981 ccgtgccgac cgcagtcggc gcagcgatgg aaggtgtcga acagcgcttc ggcgtggcca
     2041 attcggccgt gttcgccgac gcgacgaccc tgacgtccac cggtgcggtc aactcgttcc
     2101 acgactccta caccagcctc ggcggcatga tcacgctgtt caacatgcag ctcggtgagg
     2161 tcgcgccagg cggcaccggg tcgggtctct acggcatgct catcctggcc atcatcaccg
     2221 tgttcgtcgc gggcctgatg gtgggccgga caccggaata cctcggcaag aagatcaccc
     2281 cgcgtgagat caagctcgcc gcaacgtatt tcctgatcac cccgctgatt gtgttgaccg
     2341 gtactgcagt ggctatggct ctgcccggcc agcgcgccag catgctcaac accggaccgc
     2401 acggtctgtc ggaagtgctg tacgcgttca catccgcggc gaacaacaac ggatcggcat
     2461 tcgcaggcat ctccgtcaac accgagtggt acaacaccgc actggggctg gcgatggcgt
     2521 tcggccgctt ccttccgatg atcttcgtgc tggcgctggc cagctcgttg gccaagcagg
     2581 gcaaggcgcc agaatccatc ggcacattgc ccacccatcg acctcagttc gtcggcatgg
     2641 tcgccggcgt gacgttgatc cttgtcgccc tcaccttcct gcccatgctt gcgctcgggc
     2701 ctctcgctga aggaattcac tgatgtccac gcccacaatt cattccacgc cttcgcaggc
     2761 gaaggccggc acgccgaaac gtgttcaagg cgggttgctc gacccgaaga tgctgtggaa
     2821 gtcgctgccc gacgcgctgc gcaagctcga cccccgcacg ctatggcgca acccggtgat
     2881 gttcatcgtc gagatcggag cggtctggag cacgatcctg gcgatcgcgg atccctcctg
     2941 gttcggttgg ctgacggtga tatggctctg gttgacagtc gttttcgcca acgtggccga
     3001 ggcggtcgcc gagggtcgag gcaaggcaca ggcagagagt ctgcgccgga cgaagaccgc
     3061 gacaatggcg cgtcggctcg tcgactggac gcctggcgcg cctggccgcg aggaagaagt
     3121 cgccgctcct ctcctgcagc agggtgacat cgtcgtggtc gaagccggcc agaccatccc
     3181 cggcgatggt gacgtcgtgg aaggcattgc ttccgttgat gagtcggcca tcaccggtga
     3241 atcggcaccc gtcatccgcg agtccggtgg cgaccgctcg gcggtcacgg gcggcaccac
     3301 ggtgctgtcg gaccgcatcg tcgtcaagat cacccagaag cccggcgaga gcttcatcga
     3361 ccggatgatc gcgctcgtcg agggcgccaa ccggcagaag acgccgaacg agatcgcgct
     3421 gaacatcctg ctcgcgtcgc tgacgatcat cttcgtgttc gccgtcgcga ccctgcagcc
     3481 attggcgatc ttctccaagg cgaacaaccc gggcgtgccg gattccctgt cgctcaccgg
     3541 gaatggcgta tccggcatcg tgatggtctc actgctggtc tgtctgattc cgaccacgat
     3601 cggcgcgctg ctgtcggcca tcggcatcgc cggcatggac cggctggtgc agcgcaacgt
     3661 gctggccatg tcgggtcgcg ccgtcgaggc cgcgggcgac gtgaacacgc tgctgctgga
     3721 caagacaggc accatcacgc tgggcaatcg gcaagccgcg gctttcgtac cggttaacgg
     3781 tgtgtcaccc gaagtactcg ccgacgccgc gcagttgtcg agcctcgccg acgagacgcc
     3841 ggaaggccgg tccatcgtcg tgttcgcgaa acaggagtac ggcctgcgcg cacgcacccc
     3901 gggcgaactc gacaaggcgc actgggtcga gttcagcgca cagacgcgga tgtccggcgt
     3961 cgatctggag aacggccacc ggctgcgaaa gggtgcggct agctcggtgg ccgactgggt
     4021 gcgtggcgaa ggcggccgcg tgtcgaccga actcggcgac atcgtcgacg ggatctccgc
     4081 tgccggcggc accccgctgg tagtcggcga ggtaatcgag ggacaagcac gcgtcctcgg
     4141 cgtcattcac ctcaaggacg tcgtcaagca gggcatgcgc gagcggttcg acgagatgcg
     4201 ccggatgggc atccggacgg tgatgatcac cggtgacaac ccattgaccg ccaaggcaat
     4261 tgcggacgag gcaggtgtcg acgacttcct tgccgaagcc acgccggaag acaagatggc
     4321 gctgatcaag aaggagcagg ccggcggcaa gttggtcgcg atgaccggcg acggcaccaa
     4381 cgacgcaccg gcactggcgc aagcggacgt cggcgtggcg atgaacaccg gaacctcggc
     4441 ggccaaagag gccggcaaca tggtcgacct cgactccgat ccgaccaagc tcatcgagat
     4501 cgttgagatc ggcaagcagt tgctcatcac acggggtgcg ttgacgacct tctcgatcgc
     4561 caacgacatc gcgaagtact tcgcgatcat tccggcgatg ttcgtcgcgt tgttccccgg
     4621 cctggacctg ctgaacatca tgcggttgca tagtccgcag tcggcgatcc tgtcggcggt
     4681 gatcttcaac gccctggtca tcatcgcgct gatcccgctg gccctgcgcg gcgtgcggta
     4741 caagccgagc tcagcctccc agctcctcag ccgcaacctc ctg
//
LOCUS       DASRJA010000359        13202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_20079,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000359 DASRJA010000000
VERSION     DASRJA010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13202
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20079"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFC01_15955"
     CDS             <1..240
                     /locus_tag="VFC01_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZN81159.1"
                     /translation="LDIYLDGMPDGEIDKITHENALRLYNFDMFSRIPKEQLTVGALR
                     AQATDVDLGFRSSERLKKEGTDTVTMLDLASKIAT"
     gene            252..893
                     /locus_tag="VFC01_15960"
     CDS             252..893
                     /locus_tag="VFC01_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZN81160.1"
                     /translation="MEIDQLLTTTRSARKALDLGAPVDMDEIRECLQIALHAPNGSNQ
                     QAWRWMVVTEPDLRARIAELYCKTYLGKVGGQMIAGLMPSGTSEARLMSSTEFLVENL
                     HRVPVLVIPCYEPSYPRTDGDESFYLATLYGSIFPAVWNFQLALHSRGYGTCITTMHL
                     EHEREVGELLGIPATFVQGCLLPVAKLRTGHEFAPTPRRDLDEVIAVNEWGCQ"
     gene            890..1438
                     /locus_tag="VFC01_15965"
     CDS             890..1438
                     /locus_tag="VFC01_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN81161.1"
                     /translation="MNAELQERLARLEARTEIRDCIERYARGMDRRDREILRSAYHDG
                     AVDDHVGFVGEVDDFIDWAFAYHSTQTRYQHYLLNHIADIDGDQAHAETYYLFVGTDK
                     EPANHLTLSGGRYVDRLERRDGRWAIVDRVCVVEWNAESTSLVTDEVIAMMAPTMRVA
                     THDTSDPSYDRPLMAARPATPA"
     gene            1515..2858
                     /locus_tag="VFC01_15970"
     CDS             1515..2858
                     /locus_tag="VFC01_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN81162.1"
                     /translation="MADADAVKRSSVRGRPASKKGHRTAKLSAVTEEPTVDATRRTEI
                     LRTADTVIATTGLRSSLQQIADAAGILAGSLYHHFESKEAILVELIRRYHIDLDCVGD
                     RALERIDQPDPRPASEQITELGCEIARCAVEHRAALQMSFYEGPSTDPELVELAKRPP
                     TKIQAAMLQALRAGRWSGYIRGDVDLPTLADRLCQSMLHAGLDVVRHNASATEVARLK
                     SRIALQGLAVDLPTDTDLDKSNAFAVAQQVIDSWVDTEDDTDLKAAHVRAVARAEFGR
                     RGYEMTTIRDIAAAAGLGTGTVYRVIGSKDELLMSIMLEFGHKVGGAWTDIVRTDSTT
                     VEKLDALSWLNVNALDQFPDEFRIQLAWLRQTPPDTSTPAWSFTTRIRQMKALLSEGM
                     RAGEIQVDSPTADLLARCIIGEQWIPENILQEIGTRNALILARDTTLRGIAVRDM"
     gene            complement(2869..4068)
                     /locus_tag="VFC01_15975"
     CDS             complement(2869..4068)
                     /locus_tag="VFC01_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN81163.1"
                     /translation="MTTGANATDLYYDPFDFAIDDNPYPIWRRMRDEAPLYHNEKYDF
                     YALSRYDDVARELHNWDTYRSGKGTTMDIIMSGVEVPPGVILFEDPPLHDLHRRVLSR
                     VFTPRRMEAIEPLTRQYCVRALDELAGASRFDLITDFGALIPMRTIGYLLGIPEEGQQ
                     QIRDNTDASIGLKDGSFQSVSSATFENAYQLFADYIEWRAEHPSDDVMTQLLNAEVEE
                     DGELRPLTRIEVLTYTSMIAGAGNETTTRLIGFIGQLLAEHRDQRRELVEDFSLIPRA
                     IEEVLRYESPSPVQARTVARDTEVHGQTIAGGSIMLLLNGSANRDERQYPDGESFDIH
                     RTGSHLSFGQGLHFCLGSSLARMQARVALEEMLTRWPEWEIDLEDAEMAHTSSVRGWG
                     RLPLITG"
     gene            complement(4082..5155)
                     /locus_tag="VFC01_15980"
     CDS             complement(4082..5155)
                     /locus_tag="VFC01_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN81164.1"
                     /translation="MTSPLRTAVWSTGGVGSIAIDAIRGRPDLDLVGVWVHSPEKVGK
                     DAGTLAGIDPIGVTTTNDADALIALRPDCVVYAASGPDRDAGAVPDYLKLLEAGINVV
                     STSSTSLVYPPAYYSPQWREQMEAAAKAGSASFYASGVFPGFGSDQLALLLTTQSKKI
                     RCLKVTEVALNDHYPVADVMMNGMGFGRPLDFEPLLKTPGFIEMAWRAPIHLMAAGLG
                     VEVEEVRGTLDRRLTDRDIEVTFGTIKAGTCGAVSTRVAGVVNGREAIVVEHIIRMAR
                     DVAPDWLASEFDATYRVDIEGDPDIHCAMNVGAAEGHAAGYAAMSATAMRVVNAIPYV
                     VEAPAGLLSSLDLPNTLPCYAFE"
     gene            5284..6390
                     /locus_tag="VFC01_15985"
     CDS             5284..6390
                     /locus_tag="VFC01_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN81165.1"
                     /translation="MAERKHRVVVWSTGGIGSIAIRAIHQRPNLDLVGVWVHSPEKDG
                     KDAGELANGEPIGLAATTDADALIALKPDCVIYAASGPERDALAIPDYVKLLSAGINV
                     VTTSTTRLVNPHAYEPAEWRDQLAAAAKEGQVSLYASGIEPGFAADYLPLVLTTQSAS
                     IEKIHSYEIGLYDDYGVADIMSDAMGFGRPLDYQPWISFPGAIAGEWQGQIRLIADAL
                     GVEVQEVRETFDRAVTDRTLEVAMGTVEAGTCGALRMQAIGVVDGREAIIIEHVTRLA
                     PDVAPEWPTLPGALGYRVVITGQPDIDCTMAATLRDRRKAGIESMTSGAGAMVATAMR
                     VVNAVPYVVAAEPGLLSSVDLPLTIPRGAFSQHG"
     gene            complement(6391..6696)
                     /locus_tag="VFC01_15990"
     CDS             complement(6391..6696)
                     /locus_tag="VFC01_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HZN81166.1"
                     /translation="MSAEDLEKYETEMELSLYREYKDIVGQFSYVVETERRFYLANSV
                     ELVPRNSEGEVYFELRLADAWVWDMYRPARFVKQVRVITFKDVNIEEVEKPELRLPE"
     gene            complement(6739..7458)
                     /locus_tag="VFC01_15995"
     CDS             complement(6739..7458)
                     /locus_tag="VFC01_15995"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZN81167.1"
                     /translation="MPATWPPRTVIRKSSGLRTLESALYRGGLGPVAGVDEVGRGACA
                     GPLVVAACVLGPNRLESLAALDDSKKLNEKERERLFPLIRRYALAYHVVFIPSTEVDR
                     RGVHVANIEGMRRAVAGLTVRPGYVLSDGFRVPGLPMPSLPVVGGDAAAACIAAASVL
                     AKVSRDRLMVKMEAEHPGYGFAEHKGYSTPAHSAALAEMGPCSQHRYSFINVRRLVTV
                     EGELVPADSLDWEPEQQSQLG"
     gene            complement(7505..8332)
                     /gene="lepB"
                     /locus_tag="VFC01_16000"
     CDS             complement(7505..8332)
                     /gene="lepB"
                     /locus_tag="VFC01_16000"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZN81168.1"
                     /translation="MTDATDSEDPITDHTPDIADSVTDEKKTEEEPKKRGALREAAIL
                     ITIAVILYYVMLTFVARPYLIPSESMEPTLHGCAGCTGDRIMVDKLTYRFGSPQPGDV
                     IVFKGPPNWNIGYKSIRSKNATVRWAQNALSFIGFVPPDENDLVKRVIAVGGQTVECR
                     ADTGLTVDDKPLNEPFLDPTTMMADPAVYPCLGNEFGPVKVPDGRLWVMGDNRTHSAD
                     SRAHCGNTPTDAQRGLLCTGDPTLGTVPIDNVIGKARFIAWPPSRWGGVHAVNPQTQ"
     gene            complement(8410..8751)
                     /gene="rplS"
                     /locus_tag="VFC01_16005"
     CDS             complement(8410..8751)
                     /gene="rplS"
                     /locus_tag="VFC01_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZN81169.1"
                     /translation="MNTLDFVDQTSLRDDIPDFGPGDTVNVHVKVIEGSKERIQVFKG
                     VVLRRQGGGIRETFTVRKESYGVGVERTFPVHSPNIDHIDVVTRGDVRRAKLYYLREL
                     RGKKAKIKEKR"
     gene            9049..9990
                     /locus_tag="VFC01_16010"
     CDS             9049..9990
                     /locus_tag="VFC01_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81170.1"
                     /translation="MLRPSKLVTATAAVVVAVPLVAGCEAKVYGTPPPPPGPQLTVIA
                     PMGTKAPLPEAPPDEPAATFAGLDGRITRATADAAKAGADITITVLDRNTGQILSNDN
                     KEAMPIASVVKLFIADDLLLQEAHRQTQLSPADRQAFDAMLRSSDDSAAEVFWNRSGG
                     SAIISRVTSRYGLGATTTPYNGRWFNTLSTTADLVKYYDKLLSGSGGLPAERANIMLS
                     NLAASTPTAPDGTMPGGIYPQRFGIPEGLFAEPVAVKQGWFCCWNGGNWVHWSTGVIG
                     PDRRYVMAIASMQPVDDVTARNTITQAVKTMFPGGRI"
     gene            complement(9987..10664)
                     /gene="trmD"
                     /locus_tag="VFC01_16015"
     CDS             complement(9987..10664)
                     /gene="trmD"
                     /locus_tag="VFC01_16015"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZN81171.1"
                     /translation="MHIDVVTIFPDYLAPLRQSLPGKAIEAGIVQLAVHDLRRWTHDV
                     HKSVDDSPYGGGPGMVMKAPVWGEALDEICSDKTLLIVPTPAGRLFGQADAQRWSAEK
                     HLVFACGRYEGIDQRVVDDAARRMRVEEVSIGDYVLPGGESAALVMIEAVVRLLPDVL
                     GNPASHRDDSHSDGLLEGPSYTRPPSWRGLDVPDILLSGDHAKIAEWRREQGLQRTRD
                     RRPDLLD"
     gene            complement(10664..11182)
                     /gene="rimM"
                     /locus_tag="VFC01_16020"
     CDS             complement(10664..11182)
                     /gene="rimM"
                     /locus_tag="VFC01_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889279.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZN81172.1"
                     /translation="MDLVVGRVVKAHGIAGELVVEIRTDDPDTRFAPGSALRGRPSRG
                     GLESRYVVESARDHSGRLLVRLEGVADRNAADSLRGTVFIVDSEDLPPIEDPDEFYDH
                     QLEGLQVVTTSGVAVGSVAEVLHTAAGELLSVRTEKGEVLVPFVSAIVTSVSLADQTI
                     EIDPPDGLLELS"
     gene            complement(11189..11431)
                     /locus_tag="VFC01_16025"
     CDS             complement(11189..11431)
                     /locus_tag="VFC01_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZN81173.1"
                     /translation="MSSVVVDAVEHLVRGIVDNPDDVRVDMVTSRRGRTVEVHVHPDD
                     LGKVIGRGGRTATALRTLVAGIGGRGIRVDVVDTDQ"
     gene            complement(11440..11973)
                     /gene="rpsP"
                     /locus_tag="VFC01_16030"
     CDS             complement(11440..11973)
                     /gene="rpsP"
                     /locus_tag="VFC01_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZN81174.1"
                     /translation="MAVKIKLTRLGKIRNPQYRIAVADARTRRDGRAIEVIGRYHPKE
                     DPSLIEINSERAQYWLGVGAQPTEPVLQLLKITGDWQKFKGLPGAEGTLKVKEPKPSK
                     LDLFNAALAEAEGGPSTEATQPKKKKAPAKKAAEKAEAEAPAEAEAPAEAEAAAEAEA
                     PAEAPAEAAESAESTES"
     gene            12192..12683
                     /locus_tag="VFC01_16035"
     CDS             12192..12683
                     /locus_tag="VFC01_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN81175.1"
                     /translation="MDDAAALLEIEAIKQLKARYCRYLDTKDWQAWRGIFSDDFLSDT
                     SRAGGKVIRGADDFVAFTHKSLGNRATAHQVHAPEIELTSATTARGIWALEDVVRLAP
                     GVNLRGYGHYHETYEKIDGQWRITSSKLTRLREDIFNIFVSVYLSDRIKRAVGRATRR
                     LVD"
     gene            12700..>13202
                     /locus_tag="VFC01_16040"
     CDS             12700..>13202
                     /locus_tag="VFC01_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN81176.1"
                     /translation="MTFLRERLLSTVAGQLGRPHGILSPFVARALNRGNEKAIAAAVD
                     AAEVERGGVAADIGFGGGVGLQLLLDRVGDDGVVHGIEIADDMLRRAKSKFGRDGRLR
                     LARGSLTEVPLDDGSVDALITVNTVYFISELDAACAELARVLRPGGRAVIGIGDPDVM
                     ARLPFTK"
BASE COUNT         2335 a         4350 c         4159 g         2358 t
ORIGIN      
        1 ctcgacatct acctcgacgg tatgccagat ggcgagatcg acaagatcac tcacgagaat
       61 gcgttgcggc tgtacaactt cgacatgttc tcccgcattc cgaaggagca actgacggtt
      121 ggtgccctgc gcgcccaggc caccgacgtc gacctcggct tccggtcctc ggagcggctg
      181 aagaaggagg gtaccgacac cgtgaccatg ctcgacctcg cgtcgaagat cgccacctag
      241 tcgaggcgtt ggtggaaatc gaccagcttc tcacgacgac gaggtccgca cgtaaggcgc
      301 tggacctcgg cgctccggtc gacatggacg agatccgcga gtgcctgcag atcgccttgc
      361 acgcaccaaa tggctcgaac cagcaggcgt ggcggtggat ggtcgtcacc gaaccggacc
      421 tacgggcacg aatcgccgag ttgtactgca agacctacct cggcaaggtg ggcgggcaga
      481 tgatcgccgg cttgatgccc tcgggcacct ccgaggccag gttgatgtca tcgacggagt
      541 ttctggtcga gaatctgcac agggtgccgg tgctggtgat tccgtgctac gagcccagtt
      601 acccgcgcac cgacggcgac gaatcctttt acctggccac cctttatggc tcgatcttcc
      661 cagcagtgtg gaactttcaa ctggcactgc attcgcgcgg ctacggcaca tgcatcacga
      721 cgatgcatct cgaacacgag cgagaagtcg gcgaattgct gggcattccc gcgaccttcg
      781 tccagggctg cctccttccc gtcgcaaagt tgcgcaccgg acacgaattc gcaccaacgc
      841 ctcgtcgcga tctcgatgag gtaatcgccg tcaacgaatg gggttgccaa tgaatgcaga
      901 actgcaggag cgcctagccc gcctcgaggc tcgcaccgag atccgggatt gcatcgagcg
      961 ttatgcgcga gggatggacc gccgcgatcg cgagatcctc cgctctgcgt accacgatgg
     1021 ggccgtcgac gatcacgtcg gcttcgtcgg tgaggtggac gacttcatcg attgggcgtt
     1081 cgcctatcac tcgacgcaga ccaggtacca gcactacctg ctcaaccaca tcgccgacat
     1141 cgacggcgac caggcgcacg ccgagacgta ctacctgttc gtcggcaccg acaaggagcc
     1201 tgcgaatcac ctgacgctgt ctggcggtcg atacgtggac cgcttggagc gaagggatgg
     1261 ccggtgggcg atcgtcgacc gcgtctgtgt cgtcgagtgg aacgccgagt cgacgtcgtt
     1321 ggtcaccgac gaggtgatcg cgatgatggc accgacgatg agggtcgcca cccacgacac
     1381 gtccgatccc tcgtacgacc gtccgctaat ggccgcacgg cccgcgacac cggcgtgagc
     1441 gccgaccgtt ccgcgctcgg taggatcaat cctctggtct gcacccccga cgatcggcga
     1501 aggaggactc agcgatggcc gacgccgacg ccgtgaagcg gagttccgtc cgcggccgtc
     1561 ccgcgagcaa gaagggccat cgcaccgcca agctctctgc ggtcacggaa gagccaacgg
     1621 tggatgccac caggcgcacg gagatcctga gaacggccga cactgtcatc gccacgacgg
     1681 gcctgcggag ctcgctgcag cagatcgccg acgccgcagg catcctcgcc ggcagcctgt
     1741 atcaccattt cgaatccaag gaagcgatcc tggtcgaact gatccggcgc taccacatcg
     1801 atctcgactg cgtcggtgat cgcgccctcg aacgcatcga tcagcccgat ccccgtccgg
     1861 cctccgagca gatcacggaa ctcggatgcg agatcgctcg ctgcgcggtg gaacatcgcg
     1921 cagctcttca aatgtcgttc tacgagggtc cgagcaccga cccggagctc gtcgaattgg
     1981 cgaaacggcc gccgacgaaa attcaggccg cgatgttgca ggcgttacgc gccggccgct
     2041 ggagcggata catccgagga gacgtcgacc tccccacgct ggccgatcgc ctctgccaaa
     2101 gcatgttgca cgccggtctc gacgtcgttc gccacaacgc ctcggccacc gaagtggcca
     2161 ggctgaaaag ccggatcgcc ctgcaaggac tcgccgtcga tctgcccacc gacaccgatt
     2221 tggacaagtc gaacgcgttc gcagtggcgc agcaggtgat cgacagctgg gtcgacaccg
     2281 aggatgacac ggacctgaag gccgctcatg tgcgggcggt cgcacgagcc gaattcggtc
     2341 gccgcggcta tgagatgacc acgatccgcg acattgcggc agccgcgggg ttggggacgg
     2401 gcactgtgta ccgggtgatc ggctcgaagg acgagctgtt gatgtcgatc atgctcgaat
     2461 tcgggcacaa ggtcggcggc gcatggaccg acatcgttcg tacggattcg accacggtcg
     2521 agaagctgga cgcgttgagc tggctgaacg tcaacgcgct cgaccagttc cccgacgagt
     2581 tccgaattca gttggcgtgg ttgcggcaga cgcctcctga cacgtccacc ccggcatggt
     2641 cgttcaccac acgaatccgg cagatgaaag cactgctttc cgagggcatg cgggcgggcg
     2701 agatacaggt ggatagtccg acggccgacc tcctcgcccg gtgcatcatc ggcgagcagt
     2761 ggattccgga gaacatcctt caggaaatcg gcacgcggaa cgcattgatt ctcgcgcgag
     2821 acacgacctt gcggggaatc gctgtgcgcg acatgtgacc atcccttctc aacccgtgat
     2881 caggggcagt ctcccccacc cacggacact ggatgtgtgc gccatctccg cgtcctcaag
     2941 gtcgatctcc cactccggcc accgcgtcag catctcttcg agcgcgaccc gcgcctgcat
     3001 ccgcgccagc gacgacccga gacagaaatg cagtccctga ccgaaggaga gatgcgatcc
     3061 ggtgcggtgg atgtcgaaac tctccccgtc ggggtactgc cgctcgtccc ggttggctga
     3121 accgttgagt agcaacatga tcgaaccccc cgcgatcgtc tgcccgtgta cttcggtgtc
     3181 acgggcgact gtgcgggcct gcaccggcga cggcgactcg tagcgcagca cttcctcgat
     3241 cgcccgggga atcagcgaga aatcctcgac gagttcgcgt cgctggtcgc ggtgctcggc
     3301 gagcagttgg ccgatgaagc cgatcagccg agtggtcgtt tcgttgcccg cgccggcgat
     3361 catgctcgta tatgtcagca cctcgatgcg cgtcagcggt cgcagctctc cgtcctcttc
     3421 cacttccgcg ttgagcagtt gcgtcatcac gtcgtcggac ggatgctcgg cacgccattc
     3481 gatgtagtcg gcgaacagtt ggtacgcatt ctcgaaggtg gcactcgaca ccgattggaa
     3541 actgccgtct ttgaggccga tcgacgcatc ggtgttgtcc cggatctgct gctgcccctc
     3601 ttcggggatg cccagcagat agccgatggt ccgcatcggg atcagcgccc cgaagtcggt
     3661 gatcaagtcg aatcgcgagg cacctgccag ctcatcgagg gcgcggacgc agtactgacg
     3721 agtcagcggc tcgatggcct ccatccggcg cggcgtgaaa acccttgaca gcacgcgccg
     3781 atgaagatca tgtagtggtg ggtcttcgaa aaggatgacg ccgggcggca cctcgacacc
     3841 actcatgatg atgtccatgg tggtgccctt gcccgaccgg taggtgtccc agttgtgcag
     3901 ttcgcgtgca acatcgtcgt accggctcag cgcatagaag tcgtacttct cgttgtggta
     3961 gagcggagcc tcgtcccgca tccgccgcca gatcgggtac gggttgtcat cgatggcgaa
     4021 gtcgaacggg tcgtaataga gatcggtggc gttcgcgccg gtcgtcatgt cggcctcctc
     4081 atcattcgaa tgcgtagcac ggcagggtgt tcggcaaatc aagcgagctc agcagccctg
     4141 ccggcgcttc gaccacatag gggatggcgt tgaccacccg catcgcggtg gcggacatgg
     4201 ccgcgtagcc cgcggcgtgc ccctccgcgg cgccgacatt catcgcgcag tggatgtccg
     4261 gatcgccctc gatatcgacc cggtacgtcg cgtcgaactc cgaagcgagc caatccggcg
     4321 ccacgtcgcg cgccatccgg atgatgtgct cgacaacgat cgcttctcta ccgttgacca
     4381 ccccggccac cctggtgctc acggcaccgc acgtccctgc cttgatggtg ccgaacgtca
     4441 cctcgatgtc tctatcggtg agtctgcggt ccagggttcc gcgtacctcc tcgacctcga
     4501 cacccaatcc cgcggccatc agatggatcg gcgcccgcca tgccatttcg atgaaaccgg
     4561 gtgtcttcaa aagaggttcg aagtcgagtg ggcggccgaa gcccatgccg ttcatcatca
     4621 cgtcggccac cgggtagtgg tcgttgagcg cgacctcggt gaccttcagg caacggatct
     4681 tcttcgactg cgtggtcagc agcagcgcca gctggtccga gccgaacccg ggaaagacgc
     4741 cggatgcgta gaacgaggcg ctgcccgcct tcgccgcggc ttccatctgc tcgcgccact
     4801 gcggcgagta ataggccggc ggatagacca ggctggtgga cgacgtcgac acgacgttga
     4861 tgccggcttc gagcagcttc aaataatcgg gcactgcacc cgcgtcgcgg tcgggtccgc
     4921 tggcggcgta caccacgcaa tcgggtcgca acgcgatcag cgcgtcggcg tcgttggtcg
     4981 tcgtcacccc aatcgggtct atccctgcca gcgtgcccgc gtctttgccg accttctccg
     5041 gcgagtgcac ccagactccg acgaggtcga ggtcgggtcg gccccggatc gcgtctatcg
     5101 caattgatcc cacacctccc gttgaccaca cagccgttct cagcggggat gtcacaggcc
     5161 tccctttcgt tcacagaaac tcgcgacgga ctctaaccta aattttgttc ccctcgaatg
     5221 taatagtgat tactattcta aaccgatccg acagtcgcac gcggtaactc tcgaggagtc
     5281 gacatggcag aacgtaagca ccgcgtcgtg gtctggtcga cgggcggcat cggctcgatc
     5341 gcgatccgcg ccatccacca acggccgaat ctcgacctcg tcggggtgtg ggtgcactcg
     5401 ccggaaaagg acggcaagga tgccggcgaa ctggccaacg gcgaacccat cgggttggcc
     5461 gccaccacgg atgccgacgc gctcatcgcg ctgaagcccg attgcgtcat ctatgcggcg
     5521 agtggtcccg agcgcgacgc cctggcgatc cctgactacg tcaagttact cagtgcggga
     5581 atcaatgtgg tgacgacgag caccacgcga ctggtcaatc cgcatgccta cgaacccgcc
     5641 gaatggcgcg atcaactcgc ggccgcggcc aaggaaggtc aagtttcgtt gtacgcgtcg
     5701 ggcatcgagc ccgggttcgc ggccgactac ctaccgctgg tgcttacgac gcagtccgcg
     5761 tcgatcgaga agattcactc ctacgagatc gggctgtacg acgactacgg cgtcgcggac
     5821 atcatgagtg atgcaatggg attcggccgt cccctcgact accaaccgtg gatcagtttc
     5881 cccggcgcca tcgccggtga gtggcagggc caaatccggt tgatcgcaga cgctttgggt
     5941 gtcgaggtcc aggaagtgcg cgagacgttc gatcgcgccg ttacggatcg gacattggag
     6001 gtcgcgatgg gtaccgtcga ggccggaacc tgcggcgcac tgcggatgca ggcgatcggc
     6061 gtcgtcgacg gacgtgaggc catcatcatc gaacacgtca cccggctggc gcccgatgtc
     6121 gccccggagt ggccgaccct tcccggcgcc ctcggctatc gcgtcgtgat cacgggccag
     6181 cccgacatcg actgcacgat ggcggcgacg cttcgtgatc gccgcaaggc cggtatcgag
     6241 agcatgacct caggagcggg agcgatggtc gccacggcga tgcgcgtcgt gaacgcggtg
     6301 ccgtatgtcg tcgccgcaga accgggtctg ctcagctcgg ttgatctgcc gttgacgatt
     6361 ccccggggcg ctttcagcca acacgggtga ctattcgggc agtcgcagct cgggcttttc
     6421 cacctcttcg atgttcacgt ccttgaacgt gatcacgcgg acctgcttga cgaaccgggc
     6481 aggccggtac atatcccaaa cccaggcgtc ggccagccgc agctcgaagt agacctcgcc
     6541 ctcggagttg cggggcacca gctccacact gttggccagg tagaaccgcc gctcggtctc
     6601 cacgacatag ctgaactgcc cgacgatgtc cttgtattcg cggtagagcg agagctccat
     6661 ctcggtttca tacttctcga gatcttcggc actcatcggc tcagctgtcc ttcattttcg
     6721 tgatggctcc attttcccct acccgagttg gctctgctgc tcgggttccc agtcgagaga
     6781 gtccgctgga accagctcgc cttcgaccgt caccagccgg cgaacgttga tgaacgagta
     6841 tcggtgctgg ctgcagggcc ccatctcggc cagcgccgcg ctgtgggctg gggtgctgta
     6901 gcccttgtgc tcggcgaacc cgtagccagg atgttcggct tccatcttga ccatcagccg
     6961 gtcacggctc accttggcca gcacgctggc cgccgcgatg cacgccgcag ccgcgtcgcc
     7021 accgaccacc ggcagcgacg gcatcggcag gcccggcacc cggaacccgt ccgacagcac
     7081 ataaccaggc cgtaccgtca gacccgccac cgcgcgccgc atgccttcga tattcgccac
     7141 atgcacaccc cgccgatcca cctcggtcga cggaatgaac accacgtgat aggccagcgc
     7201 gtagcggcgg atcagcggaa acagccgctc gcgttccttt tcgttgagct tcttggagtc
     7261 gtcgagcgcg gccaggctct ccaaccggtt gggcccaagc acgcacgccg ccaccaccag
     7321 cggtcccgcg cacgcgccgc ggcccacctc gtcgacgccc gccaccgggc caagcccgcc
     7381 ccgatacagc gcggactcca gagtgcgcag gcccgaggac ttccggatca cagttcgcgg
     7441 aggccatgtc gcgggcaaaa gctcaagccc tccgctcgtc gcgcaagtgg ctcatcggtc
     7501 ccccctattg tgtctgcggg ttcaccgcgt ggactccacc ccatcgcgac ggcggccagg
     7561 cgatgaaccg tgcctttcca atgacattgt ctatcggcac cgtcccgagc gttgggtcac
     7621 ccgtgcacaa caacccgcgc tgagcgtcgg tcggcgtatt gccgcagtgc gcgcgcgaat
     7681 cggccgaatg ggtgcggtta tcgcccatca cccacagccg cccatcgggc acctttaccg
     7741 gaccgaactc attgcccagg caggggtaga ccgcggggtc ggccatcatg gtcgtcgggt
     7801 cgaggaacgg ctcgttgagt ggcttgtcgt ccaccgtgag gccggtgtca gcgcgacact
     7861 cgacggtctg cccgccgacc gcgatcaccc gtttgaccaa gtcgttctcg tcgggcggga
     7921 cgaatccgat gaacgacaat gcgttctgcg cccaacgcac agtggcgttc ttcgagcgga
     7981 tcgacttgta gccgatgttc cagttgggcg ggcccttgaa gacgatcacg tcgccgggct
     8041 gtggcgaacc gaaccggtag gtgagcttgt ccaccatgat ccgatcgccg gtgcacccgg
     8101 cgcacccgtg cagcgtgggc tccatcgact cggacgggat cagatagggc cgtgcaacga
     8161 acgtcagcat gacgtaatac aggatcaccg cgatggtgat caggatcgcc gcttcccgca
     8221 gcgcgccgcg cttcttgggt tcttcctcgg tctttttttc gtcggtgacc gaatccgcga
     8281 tgtcgggcgt gtggtcggtg atcgggtctt cggagtccgt ggcgtcggtc accgcatcag
     8341 agtagtcagc gcgatgcttg ccgccacgct gctcgctttc caaggatcag acctcgtcgg
     8401 cgggcgacgt cagcgctttt ccttgatttt cgccttcttg ccacgcagct cgcgcaggta
     8461 gtacagcttc gcgcggcgca cgtcaccacg ggtgacgacg tcgatgtggt cgatgttggg
     8521 cgaatggacc gggaaggtgc gctcgacgcc cacgccgtag ctttccttgc gcacggtgaa
     8581 ggtctcccgg atgccgccgc cctgacggcg aaggacgaca cccttgaaga cctggatgcg
     8641 ttccttggag ccctcgatga ccttgacatg gacgttgacg gtgtcgccgg ggccgaagtc
     8701 cgggatgtcg tcgcgtagcg acgtctgatc aacgaagtcc agcgtgttca tcggtgacac
     8761 ttccttgctg ttggcggctg cggtcagtgg gacacggtgt gacgcgtcgc cgagccgatc
     8821 tgttcgggcg tatctggggc gtctcgcaac ggacggggat ctggcccatg cggacaactg
     8881 ctcaattgtg ccagacgggc accaatccag tgaaatcgcc gccggccaaa acccccaaaa
     8941 cgcacagcag gcctaagcac gcgatgtggg cgctcagccg ctaggctgag ggcaccggca
     9001 gggcggcggg caaacctgat ccggttgcag aacacgggag gtagggcaat gctgcggccg
     9061 tcgaaactgg tcacggcaac cgcagccgtc gtcgttgcgg tcccgctcgt cgccggttgc
     9121 gaagccaaag tgtatggcac gcccccgcca cctccgggcc cgcagctaac agtcatcgcc
     9181 ccgatgggca ccaaggcccc gctgcctgaa gccccgcccg acgagcctgc cgccacattt
     9241 gctggtctcg acggccgtat cacacgggcg acggccgacg ccgcgaaagc tggcgccgac
     9301 atcacgatca ccgtcctcga ccgcaacacc gggcagatcc tctccaacga caacaaagag
     9361 gcaatgccca tcgcctcggt cgtcaagctg ttcatcgccg acgacctact gctacaggag
     9421 gctcacaggc agactcagct ctcacccgcc gaccgccagg cattcgacgc catgttgcgg
     9481 tcctcggacg atagcgcggc cgaagtgttt tggaaccgca gtggcggcag tgcaatcatt
     9541 tcgcgggtca catcgcgata cgggttgggg gcgaccacga cgccgtacaa cgggcggtgg
     9601 ttcaacacct tgagcacgac cgctgacctc gtcaagtact acgacaaatt gctctcgggc
     9661 agtggcgggt tgcctgcgga gcgggccaac ataatgctgt ccaatctggc tgcgtcgaca
     9721 ccgactgcgc ccgacggcac catgcccggc ggcatctacc cccagcggtt cggcatcccc
     9781 gaggggctct tcgccgagcc ggtggcggtc aagcagggct ggttctgttg ttggaacggc
     9841 ggtaactggg tgcattggtc gaccggcgtg atcgggccgg accgtcggta tgtcatggcg
     9901 atcgcctcga tgcagcccgt cgacgacgtc accgcgcgca acaccatcac ccaagccgtc
     9961 aaaacgatgt tcccgggcgg ccggatctag tcgagcaggt ctggcctgcg gtcgcgggtg
    10021 cgctgcaggc cttgttcccg tcgccactcc gcgatcttgg cgtgatcgcc ggacagcagg
    10081 atgtcgggca cgtcgagtcc acgccagctc ggcggtcggg tgtaactagg accttccagc
    10141 agtccgtcag agtgcgaatc atctcggtgc gacgcgggat tccccaggac atcgggcagc
    10201 aggcgcacga cggcctcgat catcacgagg gcagccgact cgcctcccgg cagtacgtag
    10261 tcgccgatcg acacctcttc gacccgcatc cgcctggccg cgtcgtcgac cacacgctga
    10321 tcgatgccct cgtaccggcc acacgcgaac accaggtgct tctcagcgct ccagcgttgc
    10381 gcgtcggcct gcccgaagag ccggcctgct ggcgtaggga cgataagaag tgttttgtcc
    10441 gagcagattt cgtcgagcgc ttcgccccac accggcgctt tcatcaccat gccggggccg
    10501 ccgccgtacg gcgagtcatc gacggactta tgtacgtcgt gggtccagcg acgcagatcg
    10561 tgcaccgcca attgcacaat gccggcctca atcgccttgc ccggcaacga ctgtcgcagc
    10621 ggggccaggt agtccggaaa tatcgtgacg acatcaatgt gcatcagctc agctccagca
    10681 ggccgtcggg cgggtcgatc tcgatcgtct ggtcggcgag agataccgac gtcacgatgg
    10741 cactgacgaa cggcaccaac acctcaccct tctcggtgcg cacggatagc aattcgcctg
    10801 cggccgtgtg caatacctcg gcgaccgagc caacggccac gcccgacgtc gtcaccacct
    10861 gcagcccttc gagctggtgg tcgtagaact cgtcgggatc ctcgatcggc ggcagatcct
    10921 ccgagtcgac gatgaacacg gtgccccgca acgaatcggc cgcattgcga tcggccaccc
    10981 cttcgagccg caccagcagc cgcccactgt ggtcacgggc ggactcgacg acgtagcgac
    11041 tttccagacc gccacgagat ggcctgccgc gcaaggcgga accgggagcg aagcgggtgt
    11101 cggggtcgtc ggtgcggatc tcgacaacga gctcgccggc gatgccgtgc gctttgacga
    11161 cccgcccgac taccaggtcc atgaatcgct actggtcggt gtccaccacg tcgacgcgaa
    11221 tgccgcggcc accgatgcct gcgacaagcg tgcgcagcgc ggtcgcggtg cgaccgccac
    11281 ggccgatgac cttgccgagg tcgtcgggat gcacgtgcac ctcgacggtg cgtccgcgcc
    11341 ggctcgtcac catgtcgacc cggacatcgt cggggttgtc cacgatcccg cggaccaggt
    11401 gctcgacggc gtcaacgacg acagagctca tggccgcggt cagctctcgg tggattcggc
    11461 cgactcggca gcctcagccg gcgcctcggc gggtgcctca gcctcggcgg ctgcttccgc
    11521 ctcagcgggt gcctcagcct cagcgggtgc ctcagcctcg gccttctcgg cagccttctt
    11581 ggcgggcgcc ttcttcttct tgggctgggt ggcctcggtg ctcgggccgc cttcggcctc
    11641 ggccagcgcg gcgttgaaca gatccagctt gctgggcttg ggctccttga ccttcagagt
    11701 gccctccgcg ccgggcagac ccttgaactt ctgccaatcg ccggtgatct tcagcagctg
    11761 caggacgggt tctgtgggct gggcgccgac gccgagccag tactgggccc gctccgagtt
    11821 gatctcgatg agactgggat cttctttcgg gtggtaccgg ccgatgacct cgatggcgcg
    11881 gccatcgcgg cgggtgcgcg cgtcggcgac ggcgatgcgg tactggggat tgcgtatctt
    11941 gccaagccgg gtgagcttga tcttgacagc catgatgaag cgatctctcc tgtgattgcc
    12001 acgctgcaat tcagcgatcg cgggcgggat gcccggatcc ggttttgcct tacgtgtgtg
    12061 accgccacgc agccgtagtc gcgaggcaga cagccgccca ttgtgccaga acggccccta
    12121 ccggtgaaaa tccatccgcg cgctgcacga ccgaccttta caatttacag tgaaagtgtc
    12181 acgctctggc tatggatgac gcggctgccc tgctggaaat cgaggcgatc aagcagttga
    12241 aggcccggta ctgccgatac ctcgacacca aggactggca ggcctggcgc gggatctttt
    12301 ccgacgactt cctcagcgac acctcgcggg cggggggcaa ggtgatccgc ggcgccgacg
    12361 acttcgtcgc gttcacgcac aagagcctcg ggaaccgcgc caccgcgcac caggtgcacg
    12421 cgccggagat cgagctgacg tcggcgacga cggcacgagg catctgggcg ctcgaggacg
    12481 tcgtccggct ggccccgggc gtcaatctgc gcggctacgg ccactaccac gagacgtacg
    12541 agaagatcga cgggcaatgg cggatcacca gctccaagct gacgcggctg cgggaggaca
    12601 tcttcaacat tttcgtgtcc gtatacctgt cggaccgaat caagagggcc gtcgggaggg
    12661 ccacgcgtcg gctggtggac tgaccgtcat gctggatgca tgacgttctt gcgcgagcgg
    12721 ttgttatcga cggtggccgg ccagctgggc cgcccgcacg gcatcttgag tccgttcgtc
    12781 gcgcgtgcac tcaaccgggg caacgagaag gcaatcgccg ccgcggttga cgcagcggag
    12841 gttgagcgcg gcggcgtggc cgcggacatt ggtttcggcg gtggcgtcgg actgcaactc
    12901 ctgttggatc gcgtcggcga cgacggcgtc gtacatggca tcgagatcgc cgacgacatg
    12961 ctgcgccgcg ccaagtcgaa gtttggccgc gacggtcggt tgcggctagc ccgcggctcg
    13021 ctgaccgagg ttccattgga tgatggttcg gtcgacgcgc tcatcaccgt caacaccgtg
    13081 tatttcatct ccgaactcga tgcggcgtgc gccgaactcg cgcgagtgct gcggccgggc
    13141 ggccgcgcgg tgatcggcat cggcgatcca gacgtgatgg cgcggctgcc gttcacgaag
    13201 ca
//
LOCUS       DASRJA010000360         6089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_349523,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000360 DASRJA010000000
VERSION     DASRJA010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349523"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /gene="dacB"
                     /locus_tag="VFC01_16045"
     CDS             <1..1173
                     /gene="dacB"
                     /locus_tag="VFC01_16045"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HZN81177.1"
                     /translation="RLAATLAPLVADPNLGALTGRITDAITGAQLWAQRADVPMQPAS
                     VNKVLTTAAALLALDRDARLTTRVMTSGQPGVVVLKGGGDPVLSAAPAGQLTWYKGAA
                     RISDLADQVLRSGVDVTTVQVDVSAYSGPTMAPGWDPADIDGGDIAPMEAVMLDGGRT
                     QPVSVESRRSTTPALEAGRALAVALKVDPATVTVLPSGLRGGQEIASVQSAPLIERLR
                     QMMNDSDNVMAESIGREVAAAQNRPQSFDGAVQAVLSQLDKGKIDTSNARLLDSSGLS
                     VDDRLTAETLDGVVNAAAGNDEPKLRPLLDLLPIAGGSGTLSNRYLDTDAGRAALGYL
                     RAKTGSLTGTNSLAGIVTDASGRVLTFAFISNNAGPTGRTAIDALASALRSCGCST"
     gene            1170..2222
                     /locus_tag="VFC01_16050"
     CDS             1170..2222
                     /locus_tag="VFC01_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HZN81178.1"
                     /translation="MSPSTKPSLTVGRAVDWDFAATVGAKLARPGPAATDYTHRQVID
                     QLADASRKAELPVREVTGLAEGGEIPEARIVDRVEWIRAATKSMRVMTGGTDTPSGFI
                     TGRITGAQTGAVLAFISSGILGQYDPFGPNGGELLLVYPNVIAVERQLRVLPADFRLW
                     VCLHEVTHRVQFRANPWLADLMSQSLAVLTDDVSENVGEVIGRLAELLRNRRDGVAPE
                     PNSTGILGLLRAVQSEPQRHALDQLLVLGTLLEGHADHVMDAVGPAVVPSVATIRRRF
                     NERRQRKQPPLQRIVRALLGFDAKLSQYTRGKAFVDHVVGQVGMARFNAIWSNAETLP
                     LPTEIDEPKRWIDRVL"
     gene            2201..3163
                     /gene="tilS"
                     /locus_tag="VFC01_16055"
     CDS             2201..3163
                     /gene="tilS"
                     /locus_tag="VFC01_16055"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZN81179.1"
                     /translation="MDRPRAVATLRAAVAAFVMEHAADDERWCVALSGGADSLALTAV
                     AAATKPTTALIVDHRLQPDSGQVAATARDQALALGCVDAQVLCVDVGTTGGPEAAARS
                     ARYRALDDARGDAAVLLAHTLDDQAETVLLGLGRGSGSRSIAGMRPCDPPWYRPLLGI
                     RRAITHAACEELGLTPWRDPHNSDRRFTRTRLRTEVLPLLEDMLGGGVAEALARTATA
                     LREDTDTLDELAGQALAEVGSGGALDTARLAVLPEAVRRRVIRGWLLAGGASGLTDKQ
                     IRGVDTLVTGWRGQGGVAVGSPLRDQRLIAGRRDGMLTLHTEPV"
     gene            3160..3762
                     /gene="hpt"
                     /locus_tag="VFC01_16060"
     CDS             3160..3762
                     /gene="hpt"
                     /locus_tag="VFC01_16060"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZN81180.1"
                     /translation="MTVWHAVGVAEQPAELYPGDIKSVLLSSDAIQAKVAELGAKIGE
                     DYRDTVGPQDLLLITVLKGAVFFVTDLARAIPVPTQLEFMAVSSYGSSTSSSGVVRIM
                     KDLDRDINDRDVLIVEDVVDSGLTLSWLLRNLATRGPRSLRVCTLLRKPDAARANVDI
                     EYVGFDIPNEFVVGYGLDYAERYRDLPYIGTLDPKVYEEE"
     gene            3831..6056
                     /locus_tag="VFC01_16065"
     CDS             3831..6056
                     /locus_tag="VFC01_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN81181.1"
                     /translation="MTETALRICPLCEATCGLTLTIENGRVTGARGDREDVFSHGFIC
                     PKGASFAELDNDPDRLTRPLVRRDGQLTEATWDEAFAVIAERLGGVVRDHGGTSVGVY
                     LGNPNAHTIAGSLYAPLILRALRTRQAFTASTLDQMPKHVALGYMFGSPVAFTVPDLD
                     RTDYLVIIGANPLVSNGSLATAADFPGKLRALRKRGGRLTVIDPARTRTAELADHHIA
                     PRPGTDAALLFAVVHTLFEEDLVAPDLGGLAEHVAGVEQVRELADGFSPEDVAAHCGV
                     PADEIRALAREIAAAPSAAVYGRIGTSTVEFGTIGSWLVDVINVLTGNLDRPGGAMFP
                     LSPVAPAPRPAGPGKGFVTGRWHSRVSGYPEALSELPAAALAEEIDTPGDGQIKAMIT
                     IAGNPVLSAPDGDRLDRALESVDFMLSIDPYLNETTRHADVILPPPPPSQTGHFDFAL
                     NNLAVRNNARYSPPALPSEGRPDEPEILSRLALILYGMGYDGDYTLVDEQVIATTLTK
                     ETADPTSPVAGRAVDELTAMLIAGPGYERRLDMMLRLGPYGDAFGANPDGLTLEKLKA
                     TPHGIDFGPLQPRVPEVLKTRSGRIELAPEPLTADIARLRESLSRGDDGFLLIGRRHL
                     RSNNSWMHNLPALAGGTNRCTLRIHPDDAAELGLTDNASIKGPGGDLVAPVELEPGMR
                     RGVVSLPHGWGHDRGGTRQGVAADQPGVNVNQLNDGGQLDPLSGTAVLNGIPVVIAPA
                     G"
BASE COUNT          984 a         2102 c         2065 g          938 t
ORIGIN      
        1 cggctggccg cgacgctggc accgttggtg gccgacccga acctcggcgc gctgaccggc
       61 aggatcaccg acgcaatcac cggggcgcag ctgtgggccc agcgcgccga cgtgccgatg
      121 cagcctgcgt cggtgaacaa ggtgctgacc accgcggcgg cgctgctcgc tctcgaccgc
      181 gacgctcggc tgaccacccg ggtgatgacc agcgggcagc ccggcgtcgt cgtgctcaag
      241 ggcggcggcg acccggtact gtcggcggcg ccagccggcc agctgacctg gtacaagggc
      301 gcggctcgga tcagcgacct cgccgatcag gtgcttcgta gcggcgtcga tgtgaccact
      361 gtgcaggtcg acgtcagtgc ctacagcgga ccgaccatgg cgccgggttg ggatccggcc
      421 gacatcgacg gcggcgacat cgcgccgatg gaagcggtga tgctcgacgg cggccgcacc
      481 cagccggtga gcgtcgaatc gcgccggtcc accacgcctg cgctggaggc gggacgcgcg
      541 ctggccgtcg cgctgaaggt cgaccccgcg acggtcacgg tgctgccctc agggttgcgg
      601 ggcggccagg agatcgcgtc ggtgcaatcg gcgccgctga tcgagcggct gcggcagatg
      661 atgaatgact ccgacaacgt gatggccgag tccatcggcc gtgaggtggc cgcggcacag
      721 aaccggccgc agagtttcga cggcgccgtg caggcggtgc tgagccagct cgacaagggc
      781 aagatcgaca ccagcaacgc ccgactgctc gactccagcg ggctgtcggt ggacgaccgg
      841 ctgaccgccg agacgctcga tggagtggtg aacgccgccg cgggcaacga cgagcccaaa
      901 ctgaggccgc tcctggacct gctgccgatc gcgggcggca gcggcacgct gtccaaccgg
      961 tatctcgaca ccgatgcggg ccgcgccgcc ttgggatacc tgcgggccaa gacgggatcg
     1021 ttgaccggaa ccaattcatt ggccggcatc gtcaccgatg ccagcgggcg ggtgctcacc
     1081 ttcgcgttca tctccaacaa cgcggggcca accggccgca cggcgatcga cgcgctggcc
     1141 tccgcgctgc ggtcttgcgg gtgcagcaca tgagcccgtc gaccaagccc agcctgaccg
     1201 tcggccgcgc cgtcgactgg gacttcgccg cgaccgtcgg cgcgaaactc gcccgccccg
     1261 gacccgcggc caccgactac acccacaggc aagtcatcga tcagctggcc gacgcgtctc
     1321 gcaaggccga gctgccggtc cgtgaggtga ctgggctcgc cgagggcggc gagattcccg
     1381 aggcccgcat cgtcgaccgc gtcgaatgga tccgcgccgc aacgaaatcc atgcgggtga
     1441 tgacaggggg aaccgataca cccagcggct tcatcaccgg ccgcatcacg ggggcgcaga
     1501 ctggcgccgt gctcgcgttc atctcgtcag gcattctggg ccaatacgac ccgttcgggc
     1561 cgaatggtgg cgagctgctg ctggtgtatc cgaacgtgat cgccgtcgag cgtcaattgc
     1621 gggttctgcc agccgatttc cggctatggg tatgccttca cgaggtgacc catcgcgtgc
     1681 agttccgggc caacccatgg ctggccgacc tcatgtcgca gtcgctcgcg gtgttgaccg
     1741 acgatgtcag cgagaacgtc ggcgaggtga tcgggcggct ggccgagctg ctccgaaaca
     1801 ggcgcgatgg ggttgcgccg gaaccgaatt cgactggaat tttgggattg ctgcgggcgg
     1861 tgcagtcgga gccccagcgg catgcgctcg atcaattgct tgtccttggc acgctgttgg
     1921 agggtcacgc cgaccatgtg atggacgccg tcgggccggc cgtggtgccc tcggtggcga
     1981 cgattcggcg ccgtttcaat gagcgacgcc aacgcaagca accgccgctg cagcgcatcg
     2041 tgcgggcgct gctggggttc gacgccaagc tcagtcagta cacccgcggc aaggcgttcg
     2101 tcgaccatgt cgtcggccag gtcgggatgg ccaggttcaa cgccatctgg tccaacgccg
     2161 aaaccctgcc gctgccaacc gaaatcgacg aaccgaaacg atggatcgac cgcgtgctgt
     2221 agccacgctg cgggcggccg tcgcggcgtt cgtgatggag cacgccgcag acgacgaacg
     2281 ctggtgtgtc gcgctgtccg gcggtgcgga ttcgctggcg ctcaccgccg ttgcggccgc
     2341 gaccaagccg accaccgcgt tgatcgtcga ccaccgactg caacccgact ccgggcaggt
     2401 ggctgcgacc gcgcgcgacc aggcattggc gttgggatgt gttgacgccc aggtgctttg
     2461 cgtcgacgtc ggcacaacgg gtggaccaga agcggccgcc cgctccgccc ggtaccgcgc
     2521 gctggacgac gcccgaggag atgccgccgt gctgcttgca cacacactcg acgaccaggc
     2581 cgagacggtg ttgctcgggt tgggccgtgg ctcggggtcg cggtcgatcg caggcatgcg
     2641 gccgtgcgat ccgccgtggt atcgcccgct gctgggcatc cgccgtgcga tcactcatgc
     2701 cgcatgcgaa gagttggggc tcacgccctg gcgagaccca cacaacagcg atcgtcggtt
     2761 cactcgcacg cggctgcgca ccgaagtgct gccgctgctg gaggacatgc tcggcggcgg
     2821 ggtcgccgaa gcgctggccc gcaccgcgac ggcgctgcgt gaagacaccg acaccctcga
     2881 cgaactggcc gggcaggcgc tggccgaggt gggctccggc ggtgccctcg acaccgcccg
     2941 gctggcggta ttgccggagg cggtccgacg cagggtgatt cgtggctggc tactggcggg
     3001 cggtgccagc gggttgaccg acaagcagat ccgcggggtg gacaccctgg tcacggggtg
     3061 gcgcgggcaa ggcggcgtgg ccgtcgggtc gccgctgcgc gaccagcgac ttatcgccgg
     3121 ccgccgcgac gggatgctga cgctgcacac cgagccggtg tgacggtatg gcacgctgtg
     3181 ggcgtggccg agcaacctgc cgagctgtat cccggtgaca tcaaatcggt gttgctttcg
     3241 tcggacgcga tccaggcaaa agtcgccgaa ctcggcgcga agatcggcga ggactaccgc
     3301 gacaccgtcg gcccgcagga tctgctgctg atcaccgtgc tcaagggcgc ggtgtttttc
     3361 gtcaccgatc tcgcgcgggc gattccggtg cccacccagc tcgagttcat ggcggtcagt
     3421 tcctacggct cgtcgacgtc atcgtcgggt gtcgtgcgca ttatgaagga cctcgaccgc
     3481 gacatcaacg atcgagacgt gttgatcgtc gaggacgtcg tcgactccgg tctgacgctg
     3541 tcctggctgc tgcgcaacct cgcgacgcgg ggtccgcggt cgctgcgggt gtgcacgctg
     3601 ctgcgtaaac ccgacgccgc gcgggccaac gtagacatcg agtacgtcgg cttcgacatc
     3661 cccaacgagt tcgtcgtcgg ctacgggctc gactatgccg agcggtaccg cgacctgccg
     3721 tacatcggca cactggaccc caaggtgtac gaggaggagt agtcggcggc caccggggat
     3781 tacgtaccgg gtaatggcgc ggacgccgga tgtcggcgca agatgacggg atgaccgaga
     3841 ctgctctgcg gatctgtcca ctgtgtgagg ccacctgcgg cctgacgctc accatcgaga
     3901 acggccgcgt gacgggagct cgcggcgacc gcgaggacgt cttcagccac ggcttcatct
     3961 gccctaaggg tgccagcttc gccgagctcg acaacgatcc cgaccggttg acacgcccgc
     4021 tggtacgtcg tgacggccag ctgaccgaag cgacgtggga cgaggccttc gccgtcatcg
     4081 ccgagcggct cggcggagtc gttcgtgatc acggcgggac gtcggtcggc gtgtatctcg
     4141 gcaatccgaa cgcccacacg atcgccggca gtctgtacgc accgctgatc ctccgcgcgt
     4201 tgcgcacccg ccaggcgttc accgcgagca ccctggacca gatgcccaag cacgtcgcgt
     4261 tgggctacat gttcggcagc cccgtcgcgt tcaccgtgcc cgatctcgac cgaaccgact
     4321 atctggtcat catcggcgcg aatccgcttg tgtccaatgg cagtttggcc accgccgcgg
     4381 acttcccggg caagctgcgc gcgttgcgca agcggggcgg ccgactcacc gtcatcgacc
     4441 cggcccgcac ccgcaccgcc gaactggccg accaccatat cgccccacgg ccaggcaccg
     4501 acgccgcgct gctgttcgcg gtggtgcaca cgctgttcga agaagacctt gtcgcaccgg
     4561 atctcggcgg actggccgag catgtcgccg gcgtcgagca ggtccgcgaa ctggccgacg
     4621 gtttctcgcc ggaggacgtc gccgcccact gcggtgtgcc cgccgacgag atccgcgccc
     4681 tcgcccgcga gatcgccgcc gctccgtcgg ctgcggtcta cggccgaatc ggtacctcca
     4741 cagtcgaatt cggcacgatc ggcagctggc tcgtcgacgt catcaacgtc ctcaccggta
     4801 atctcgaccg gcctggcggt gcgatgttcc cgctctcgcc cgtcgcaccc gcgccgcgac
     4861 ccgcggggcc gggcaagggc tttgtgaccg gacgctggca cagccgtgtc tcggggtatc
     4921 cggaggcgct gtcagagctt ccggccgccg ctctcgccga agagatcgac acccccggcg
     4981 acggccagat caaggcgatg atcacgatcg ccggcaaccc cgtgctgtcg gcacccgacg
     5041 gcgatcgcct cgaccgggcg ctcgagagcg tggatttcat gctcagcatc gacccgtacc
     5101 tcaatgagac gacgcgccac gccgatgtga tcctgccgcc tccgccgccc tcgcagaccg
     5161 ggcatttcga cttcgcgctc aacaacctcg cggtgcgcaa caacgcccgc tactcgccgc
     5221 cggcgctgcc gtccgaaggc cggcccgacg agcccgagat cctgtcccgg ctcgcgctga
     5281 tcctctacgg catgggatac gacggtgatt acacgctggt cgacgaacag gtcatcgcaa
     5341 ccacattgac gaaggagaca gccgacccga cctcacccgt cgccgggcgt gcggtcgacg
     5401 agctgaccgc gatgctcatc gccggaccgg gatacgaacg tcgtctcgac atgatgcttc
     5461 ggctgggccc ctacggagat gcgttcggcg ccaatcccga cggtctcact ctggagaagc
     5521 tgaaagccac gccgcacggc atcgacttcg gcccgctgca gccgagagtt cccgaagtcc
     5581 tcaaaacacg ttcgggccga atcgaactcg cgcccgaacc gctgaccgct gatatcgcga
     5641 ggcttcgcga atccttgagt cgcggcgacg acggctttct gctgatcggt cgcaggcatt
     5701 tgcgttcgaa caacagctgg atgcacaacc tgccggccct cgcgggcggc acgaaccgct
     5761 gcacactgcg gatccatcca gacgacgccg cggaactggg actgaccgac aacgcatcca
     5821 tcaagggtcc cggcggcgat ctggtggcgc cggtcgaact ggagccgggc atgcgtcgcg
     5881 gcgtcgtctc gctgccgcac ggctggggtc acgaccgcgg cggaacccgc cagggcgtcg
     5941 ccgccgacca gcccggcgtc aacgtcaacc agctcaacga cggcggtcag ctcgacccgc
     6001 tgtcgggcac ggctgtgctc aacggcattc cggtcgtcat cgcgcccgcg ggctagccgc
     6061 cagagcgtca ggtcagctcg aaggccacc
//
LOCUS       DASRJA010000361        10636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_217839,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000361 DASRJA010000000
VERSION     DASRJA010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10636
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217839"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..931
                     /locus_tag="VFC01_16070"
     CDS             26..931
                     /locus_tag="VFC01_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZN81182.1"
                     /translation="MDVTLALDVGGTKIAVGLVDADGTLVHHAKLPTPDGDAEAVWAV
                     VDTLVTEALAEARGGVRGVGISSAGPIDLPSGTVSPINIIEWQRFPIVERVSALTGAP
                     VRLGGDGLCMALGERWRGAGRGAQFLLGMVVSTGVGGGLVLDGAPYDGRTGNAGHVGH
                     VVVDPDGGPCTCGGHGCVETIAAGPRMAQWARENGWQAPAEADAKELADAANAGDAVA
                     LRAFRRGATAVAAMIASVGAVCDLDLVVIGGGVAKSGPLLFDPVREALAGYARLDFLR
                     GLRVLPAELGGDAGLVGAAALVHIG"
     gene            complement(928..1518)
                     /locus_tag="VFC01_16075"
     CDS             complement(928..1518)
                     /locus_tag="VFC01_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN81183.1"
                     /translation="MAIEDRRERERTARREMIIATARRLAEAEGWDAVTTRRLSTEIE
                     YSQPVLYKHFSGMEQISQAVAVDGFAELADVLGAARSGAGTPSVTLTRMARAYLDFAR
                     DNPAVYDAMFTRATTLRFGTNDTPAQLTAAFEELRQVVDVVADGRDVDALTELYWATL
                     HGLVTLTRGGRLRPGYESERLQMLVNQLTPGPEMTH"
     gene            1618..2094
                     /locus_tag="VFC01_16080"
     CDS             1618..2094
                     /locus_tag="VFC01_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HZN81184.1"
                     /translation="MDLDLVTRVAVLIAAMGTAVVYGTDVFCAVVLRPALASVDDRVV
                     VAVTGNVHRYGDRRMPVPGGLGLLATTASAVLAAVASHWEVAIVTASALALLLVWLVL
                     YTQVSAPINRQLTAAAVANQSLANGRALQAKWDRIIGVRALLQGLALAALCVALMV"
     gene            2152..2469
                     /locus_tag="VFC01_16085"
     CDS             2152..2469
                     /locus_tag="VFC01_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HZN81185.1"
                     /translation="MFQLRMYTLRSPEALHRYATVHWARHVPTFKRFGVTTHGVWTER
                     NGGANRLVALIRYPPGADPEQLARRCMAGPEFAADMTGFDADEIVDVQSVLIDPTTFA
                     PIR"
     gene            2488..2868
                     /locus_tag="VFC01_16090"
     CDS             2488..2868
                     /locus_tag="VFC01_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4267 domain-containing protein"
                     /protein_id="HZN81186.1"
                     /translation="MTATTIAYTLAGLIGAGIIFIGARFILAPRVAAAGYGVQPDLGQ
                     RSAGAYLSVKGVRDIASGLFIFILIAAGAAHLLGWVILAATIIPLADAAIVLGHGGSK
                     SIALGVHGLTAAVMLTTSALFLLL"
     gene            3208..3735
                     /gene="rplJ"
                     /locus_tag="VFC01_16095"
     CDS             3208..3735
                     /gene="rplJ"
                     /locus_tag="VFC01_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZN81187.1"
                     /translation="MAKADKVTAVADIAEQFKEASATLVTEYRGLTVANLKDLRRSLG
                     DSATYTVAKNTLVKRAAAEAGIEGLDELFVGPTAIAFVKGEAVDAAKAIKTFAKDNKA
                     LVIKGGYMDGRPLSVAEVERIADLESREVLLAKLAGAMKANLSKAAALFNAPASQVAR
                     LVAALEEKKASEEAA"
     gene            3799..4191
                     /gene="rplL"
                     /locus_tag="VFC01_16100"
     CDS             3799..4191
                     /gene="rplL"
                     /locus_tag="VFC01_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZN81188.1"
                     /translation="MAKLSTDDLLDAFKELTLLELSEFVKKFEETFEVTAAAPVAVAA
                     APGAAAGAPAEAAEEQSEFDVILEGAGDKKIGVIKVVREIVSGLGLKEAKDLVDSAPK
                     PLLEKVAKEAAEEAKAKLEAAGASVTVK"
     gene            4365..5447
                     /locus_tag="VFC01_16105"
     CDS             4365..5447
                     /locus_tag="VFC01_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN81189.1"
                     /translation="MGIGIQVEGLTKSFGPQRIWEDVTMEVPPGEVSVLLGPSGTGKS
                     VFLKSLIGLLRPERGKIIVDGTNIIECSAKELYEIRTLFGVMFQDGALFGSMSLYDNT
                     AFPLREHTKKKESEIRNIVMEKLELVGLGGDENKFPGEISGGMRKRAGLARSLVLDPQ
                     IILCDEPDSGLDPVRTAYLSQLLIDINAQIDCTILIVTHNINIARTVPDNMGMLFRKH
                     LVMFGPREVLLTSDEPVVRQFLNGRRIGPIGMSEEKDESTMAEEQAMVDAGHHDGGVE
                     EIEGVPPQIQATPGMPERQAVGRRQQRVREILHTLPPAAQEAIRDDLEGTHKYAAHSI
                     PDAETARHRSGDDDAPTGRIPISGER"
     gene            5457..7634
                     /locus_tag="VFC01_16110"
     CDS             5457..7634
                     /locus_tag="VFC01_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN81190.1"
                     /translation="MSAEQKVTFCRICEPLCGMIATVDDGRLVSLRPDKDHPLSAGFA
                     CQKGIAFTEVINDPDRVTTPLRRRGAGFEPVSWDDAMTDIADRLAAIHKKHGAGAIGW
                     YWGNPGAFSYSHILGLNTFMLGFGPRLHVFTAGSQDVNNRFVASQLLYGSPLALPVPD
                     VLRTDFLVVIGANPIVSHGSVLTVPRIKDRMHDIVKRGGRVLVIDPRRTETAAQFEWL
                     GIVPDGDAYLLLSLLHVLFAENLVDRTRLARQADGAAWLEQLARPFSPETTAPRTGIE
                     PDTVRALARDLARTPRAAVYGRVGTSTGENGTLTTYLLDAVNLVAGNLDAVGGSMFGR
                     FGFPGERLAMKALGALLRTIYTRRRSRIGGFPSVLMSEPAGVMAKEISTPGPGQVRAL
                     FVSAGNPVLSVPNGDELEKALDELQLTVGIDLYVNETLAHCDYVLPAATMYERDDFPL
                     PFQTLQPTPFRQTTEAVIEPVGQARAEWEVIDDITRRMWRRTPGLAALAAARKVLGLF
                     AVRLSPRLLVDAVIRLGDGGDRFGLRRGGLTFSRLAADHPHGVVLAPNLRDGVLGDTV
                     VYRGRRVRLQHDEIAAEIEALRGRRAPDGYPMRLIGMREARSENSWMHNAPLLMRGDR
                     MHAARMHVDDAAAANIVDGDVVRIASPHGEIELPVTITKDIMAGVIAVPHGWGHKGTG
                     GWRVANAAGGANVNRLMSSAPEDLERLAGMAHLTGVPVRVDRA"
     gene            7665..8438
                     /locus_tag="VFC01_16115"
     CDS             7665..8438
                     /locus_tag="VFC01_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN81191.1"
                     /translation="MAQGLGRLHGKSAVITGAAFGIGRATAELFVREGARLTATDIQG
                     EPLLALADELRQGGAEVETVVGDVSIEADARRMIGAATDRFGRLDILVANAGIIPLGD
                     ATEMTAADWDNVMAIDGRGMFLTCKFAIDAMVPTGGGAIVCLSSISGLAGQKRQAVYG
                     PAKFVATGLTKHLAVEWADRGIRVNAVAPGTIRTERVKQLPDEPGGSEYLAAIEQMHP
                     MGRIGEPAEVASAIVFLASDDASFITGAVLPVDGGYLAQ"
     gene            complement(8442..>10636)
                     /locus_tag="VFC01_16120"
     CDS             complement(8442..>10636)
                     /locus_tag="VFC01_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN81192.1"
                     /translation="ELGEVLQEHDDEAERYVLTSLINEIHETGTRMTLVIDDWQRVTD
                     PATIAALLYLLVYASDDLTVVVTSRNQSGLPMSRMRMQDELLEIDATALRFDVAESEN
                     FLVDLSGLDLDRPDVEELTAKTDGWVAALQLASLSLRGRDDPERLIGTMTGRHHAISE
                     FLAENVLDTLEPSMLDFLLATSITERICGDLATALSGVPDGQAMLEQVEERDLFLRRV
                     DEQWFRYHQLFLDFLLHRLGRDEPQRVVRLHRAASTWFADNHFVSEAVDHALAAGDDE
                     RAVQLVENHGVDLVANSQMATLIGLVGKLPAAVVKTDPRLQLALAWANIVLHRIPAAE
                     QALARMESGLERCGLGDDEIADLRADGGVVRGVAELRADRLAGIDEHIAPCLARREHL
                     RPFTVGVAANVATFAAAYHYNLDEVNRIQAWAAPYAERNRDTFNTVHGLCYTGLAHQL
                     MLDITTAEACFRKALKIAKRSGGSHSYTARLASSALGELLYERGDLDEAARLLEEGYK
                     LGPEGGSVDFKIARYVIAARVKALQGDRLAAGQRLDEAIRVARSLSLNRLRAMAEHER
                     TRLGLPPHPDSDMWPPASYESRRQPVDAIDEITVQFEEASAIRLLMAADDPEKKQLAC
                     RWAQEWVDRTASPNRPQALLRARRLLGASLSAAGRVDEAKSTIASVAAQCAQLGLLRY
                     LVDGGPYVAAILSELQADQRSGCWRPEWPEVPPDFLDQAVNAEVAQRV"
BASE COUNT         1813 a         3466 c         3539 g         1818 t
ORIGIN      
        1 agcccgacaa cacccacagg cactgatgga cgtcacgctt gcactcgacg tcggcggcac
       61 caagatcgcc gtgggactcg tcgacgccga cggcacgctg gtgcatcacg ccaagctgcc
      121 cacgccggac ggtgacgccg aggcggtctg ggcggtcgtc gacacgttgg tcaccgaggc
      181 tttggccgag gcacgaggag gggtgcgcgg cgtcggcatc tcctcggccg gaccgatcga
      241 cctgccgtcg ggcaccgtca gcccgatcaa tatcattgag tggcagcggt ttccgattgt
      301 cgaacgggta tcggcactga ccggtgcgcc tgtgcggctc ggcggtgatg ggctgtgcat
      361 ggcgttgggg gagcggtggc gtggagcggg ccggggcgcc cagtttctgc tcggcatggt
      421 ggtgtcgacc ggcgtcggag gcgggctcgt gctcgacggc gcgccctacg acgggcgaac
      481 cggcaacgcc gggcacgtcg gccacgtcgt cgtcgacccg gacggtgggc cgtgcacgtg
      541 cggcgggcac ggctgtgtgg agacgatcgc cgcggggcca cgaatggcgc agtgggcccg
      601 cgagaatgga tggcaggccc ctgccgaagc cgacgcgaag gagttggccg atgccgccaa
      661 tgcaggcgat gcggtggcac tgcgggcgtt ccgccgcggc gcgacggcgg tggcggcgat
      721 gatcgcatcg gtcggggcgg tgtgcgatct cgatctggtc gtcatcggcg gcggcgtggc
      781 caagtcagga ccgctgctgt tcgacccggt gcgtgaggcg ctggctggct acgcgcggct
      841 ggacttcttg cgtgggctgc gggtgctgcc tgccgaactc ggcggcgacg cgggtctggt
      901 cggtgcggcg gcgctcgtgc acatcggtta gtgcgtcatt tccggcccgg gggtcagctg
      961 attgacgagc atttggagac gctccgattc gtagccaggg cgtagtcggc cgccacgggt
     1021 aagggtgacc aggccgtgca gcgtcgccca gtacagctcg gtgagcgcgt cgacgtcacg
     1081 cccgtcagca accacgtcga ccacctggcg tagctcctcg aaagccgccg tcagctgagc
     1141 cggtgtgtcg ttggttccga atcgcagtgt ggtcgcccgg gtgaacatcg cgtcgtaaac
     1201 ggcaggattg tctcgggcga aatcgagata ggcacgtgcc atccgggtca aggtgacgct
     1261 tggggtgcca gcgcccgagc gggcggctcc aagcacatcc gcaagctcgg cgaacccgtc
     1321 gacggcaaca gcctgtgaaa tctgttccat gccgctgaag tgcttgtaga gcacgggctg
     1381 gctgtactcg atctctgtgg acaaccggcg cgtggtgacg gcatcccagc cctcagcctc
     1441 ggccaacctt cgggccgtcg cgatgatcat ttctcggcgc gccgtccgct cacgctcacg
     1501 tcgatcctcg atggccatac cgcatcttag cagcgctaga aatgctagca ctgctattga
     1561 cgcgacgccg aaagggggtt agcgttgcta gcacttacgc ctgaagggag ttacgagatg
     1621 gacttggacc tcgtcactcg tgtcgctgtc ctgatcgcgg cgatgggtac agcggtggtc
     1681 tacggcacag atgtgttctg cgcggtcgtg ctgcggccgg cattggcatc ggtcgacgac
     1741 cgtgtcgttg tcgcagtgac gggaaacgtg catcgatacg gagacaggcg aatgcctgtg
     1801 cccggggggc tcggattact cgctaccacg gccagtgcgg tgctggccgc ggtggcttcc
     1861 cactgggaag tggcgatcgt gacggcctcc gccctggccc tcctgttggt ctggcttgtg
     1921 ctctacacgc aggtgagtgc accgatcaat cgacaactga cggcggccgc cgtcgccaat
     1981 caatcgctgg cgaatgggcg tgcgctgcaa gcaaagtggg atcgaatcat cggcgtccgc
     2041 gccctgctgc aaggtctggc actggccgcc ctctgcgtgg cgctgatggt ctgacgggac
     2101 cgagttctcc gatccacacc atcaccatcg actcaaggag tacggcattc gatgtttcag
     2161 ctgcggatgt acaccctgcg gtcgcctgaa gctctccacc ggtacgcgac ggttcactgg
     2221 gcccgtcacg tgccaacgtt caagagattc ggcgtcacca cccacggggt gtggacagag
     2281 cgaaacggtg gcgcgaatcg actcgttgcg ttgatccgct atccaccggg tgccgacccc
     2341 gaacaactcg cgcggcgttg tatggccggc cccgaatttg ccgccgacat gaccggtttc
     2401 gatgccgacg agatcgtcga cgttcagtca gtgctgattg acccgacaac attcgcaccg
     2461 attcgctgac aactaggagc gttcgacatg accgccacaa ccatcgccta cacgctcgct
     2521 ggactcatcg gcgcgggcat catattcatc ggcgcccggt tcatcctcgc gccacgcgtg
     2581 gcggccgccg ggtacggcgt gcaaccagat ctcggccaga gatccgccgg cgcctacttg
     2641 agcgtcaagg gcgtgcgcga catcgcatcc gggctgttca tattcatctt gatcgccgca
     2701 ggcgcggcgc atctgctcgg ctgggtgata ctggccgcca cgatcattcc actcgccgac
     2761 gcggcgatcg tgcttggcca cggtggctcc aagtcaatcg ccttgggtgt ccatggcctc
     2821 accgccgcag tcatgctcac cacgtctgcg ctcttcctcc ttttatagga gagcgctcgg
     2881 atttggcgca cagtcacatg ccgcgagcgt gcgcaaagtg ccacattttg gcggcgtgtt
     2941 gcgtgcagac gcgcacgctc gccgcagtag gcgaccggcg ttttggctga tcggcgggcg
     3001 gcacgctatt cttgaccagt tccaccgaag accgtcggtc accgagaaat cggttgaagg
     3061 tccgggatta cccggcggcc cacgcaggag gacgaggcac taacgctgta tttgcgcgcc
     3121 ccgacctgtc tgcgtcgggg cgttcgtcat ttccggccac cttcggttcg aggcgggaca
     3181 cgcgatacca accacgagga ggcatgcatg gccaaggctg acaaggtcac cgcggttgcc
     3241 gacatcgccg agcagttcaa ggaggcgagc gcgacgctcg tcaccgagta ccgcgggctg
     3301 acggtggcca acctcaaaga cctgcgcagg tcgctcggtg attccgccac ctacacggtc
     3361 gccaagaaca cgctggtcaa gcgtgccgcg gcggaggcgg gcatcgaggg tctcgacgag
     3421 cttttcgtcg gtcccaccgc gatcgcgttc gtcaagggtg aggcagtcga cgccgccaag
     3481 gcgatcaaga ccttcgccaa ggacaacaag gcgctggtca tcaagggcgg ctacatggac
     3541 ggtcgcccgt tgagcgtggc cgaggtcgag cgaatcgccg acctggagtc ccgcgaggta
     3601 ctgctggcca agctggccgg cgcgatgaag gcgaatttgt cgaaggctgc cgccctgttc
     3661 aacgctccgg cgtcgcaggt cgcccggctc gtcgccgcac tggaagagaa gaaagccagc
     3721 gaggaagccg cgtagaagct tcctcaaagt ccgaatcaat cccactagcc cagaagaaag
     3781 aatcaggaag gaccaaacat ggccaagctg tccaccgacg acctgctcga cgcgttcaag
     3841 gaactgacgc tgctcgagct ctctgagttc gtcaagaagt tcgaggagac cttcgaggtc
     3901 accgccgccg ctccggtcgc tgttgcggcc gctcctggcg ctgccgcggg tgcgcccgcc
     3961 gaggccgccg aggagcagtc ggagttcgac gtcatcctcg agggtgccgg cgacaagaag
     4021 atcggcgtca tcaaggtcgt ccgcgagatc gtctccggcc tgggcctcaa ggaggccaag
     4081 gacctggtcg acagcgcccc caagccgctg ctcgagaagg tcgccaagga ggccgccgag
     4141 gaggccaagg ccaagctcga ggccgctggc gcttcggtca ccgtcaagta gtttctctac
     4201 gacgtggggc ggatctggca aacagatccg ccccacagtt gtatctacgg ccggtcacaa
     4261 tcggccacag acgagtgttg tgctcggacg gcgtaagcta tggtgactca agccacaggc
     4321 gggaggccgt gtggcggggt cggtttctgg cgaaaggaat tcgcgtgggc attggtattc
     4381 aggtagaggg actcaccaag tccttcgggc cccagcgaat ctgggaagac gtcacgatgg
     4441 aggtccctcc cggtgaggtc agcgtcctcc tcggcccgtc gggcaccggt aaatcggtgt
     4501 tcctgaagtc tctgatcggt ctgctgcggc ccgagcgcgg caaaatcatc gtcgatggca
     4561 ccaacatcat cgagtgctcg gcgaaggagc tctacgagat ccgcacgctg ttcggtgtca
     4621 tgttccagga cggcgcgctg ttcggctcga tgagcctcta cgacaacacc gctttcccct
     4681 tgcgtgagca caccaagaag aaggaaagcg agatccgcaa catcgtcatg gagaagcttg
     4741 aactcgtcgg ccttggcggt gatgagaaca agttccccgg cgagatctcc ggcggtatgc
     4801 gcaagcgtgc cggactggct cggtcgctgg tgctcgatcc gcagatcatc ctctgcgacg
     4861 agcccgactc cggtctggac ccggttcgta ccgcttatct gagccagctg ctgatcgaca
     4921 tcaacgcgca gatcgactgc acgatcctga tcgtcacgca caacatcaac atcgctcgca
     4981 cggtgccgga caacatgggc atgctgttcc gcaagcacct ggtcatgttc ggtccacgcg
     5041 aggtgctgct gaccagcgac gagcccgtgg tgcggcagtt cctcaacggc cgccgcatcg
     5101 gcccgatcgg catgtccgag gagaaggacg agtcgacgat ggccgaagag caggccatgg
     5161 tcgacgccgg ccaccacgac ggcggtgtcg aggagatcga gggcgtgccg ccacagatcc
     5221 aggcgacccc gggcatgccg gaacgtcagg cagtgggccg ccgtcagcag cgggtacgcg
     5281 agatcctgca caccctgccg cccgccgccc aggaagcgat ccgcgacgac ctcgagggca
     5341 cccacaagta cgccgcgcac tcgattccgg atgcggagac cgcgcggcac cgctcgggtg
     5401 acgacgacgc accgaccgga cgcatcccga tcagcggaga gcgctagcct caacgcatgt
     5461 ccgccgagca gaaggtaacc ttctgccgga tctgcgagcc gctgtgcggc atgatcgcga
     5521 ccgtcgacga cggccgactg gtgtcgctgc gtcccgataa ggaccatccg ctgtcggccg
     5581 ggttcgcatg ccagaagggc atcgcgttca ccgaggtaat caacgatccc gaccgggtta
     5641 ccaccccact gcgccgcagg ggtgcgggtt tcgaaccggt cagctgggac gacgctatga
     5701 ccgacatcgc cgaccggctc gccgcgatcc acaagaagca cggcgccggc gccatcggct
     5761 ggtactgggg caaccccggc gccttcagtt actcgcacat cctgggtctc aacacgttca
     5821 tgctcggatt cggtccgcgg ttgcacgttt tcaccgccgg atcgcaggac gtcaacaatc
     5881 gcttcgtggc cagccagctg ctgtacggct cgccgctggc attgccggtg cccgacgtgc
     5941 tgcgcaccga tttcctcgtg gtgatcggcg cgaacccgat cgtctcgcac ggcagtgtgt
     6001 tgaccgtgcc gcggatcaag gaccgcatgc acgacatcgt caagcgcggt ggccgggtac
     6061 tcgtgatcga cccacgcagg accgagaccg ccgcccagtt cgaatggctg ggcatcgtcc
     6121 ccgacggcga cgcctacctg ctgctgtcac tgctacatgt gctgttcgca gagaacctcg
     6181 tcgaccgcac ccggctggcc cgtcaggccg acggtgccgc ctggttggag cagttggccc
     6241 ggccgttcag cccggagacc acagcgccgc ggaccggcat cgagccggac accgtgcggg
     6301 cactagcccg cgatctggcg cgcactccgc gggccgccgt gtacggccgg gtcggcacca
     6361 gtactggtga aaacggcacg ctcacaacgt atttgctcga cgccgtcaac ctggtcgcgg
     6421 gcaacctcga tgcggtcggc ggtagcatgt tcggccggtt cgggtttccg ggtgagcggt
     6481 tggcgatgaa ggcgctcggc gcgctgctgc gcacgatcta cacacgcagg cgatcccgca
     6541 tcggcggctt cccctcggtg ttgatgtccg agccggcagg cgtgatggcc aaggagatca
     6601 gtacgcccgg gcccggtcag gtgcgcgcgc tgttcgtcag cgcgggcaat ccggtgctgt
     6661 cggtgcccaa cggcgacgaa ctcgaaaagg cgctggacga actgcaattg acggtcggca
     6721 tcgacctcta cgtcaacgag acgctcgcgc actgcgacta cgtgctgccc gccgcgacga
     6781 tgtacgagcg cgacgacttc ccgctgccgt ttcagacgct gcagcccacc ccgttccgcc
     6841 agaccaccga ggccgtcatc gaacccgtcg gacaggcccg cgcggaatgg gaggtcatcg
     6901 acgacatcac ccgccggatg tggcgtcgca ccccaggact ggccgctctc gctgctgccc
     6961 gaaaagtctt aggtctcttc gctgttcggc tatctccgcg gctgcttgtg gatgcggtca
     7021 tccggctcgg tgacggtggt gatcgattcg ggttgcgtcg gggcgggctc actttctccc
     7081 ggctagccgc cgaccatccc cacggcgtcg tgttggctcc gaatctgcgt gacggcgtgc
     7141 tcggcgacac cgtcgtgtac cgcgggcgcc gggtgcgact tcaacacgac gagatcgcag
     7201 cggagatcga ggcgctacgc ggccgtcgcg cacccgacgg ctatccgatg cgactgatcg
     7261 gcatgcgcga agcccgctcg gagaactcct ggatgcacaa cgcaccgctg ctgatgcgcg
     7321 gcgaccggat gcacgccgcg cgcatgcacg tcgacgacgc ggccgccgcc aacatcgtcg
     7381 acggcgacgt ggttcgaatc gcctcgccgc acggcgagat cgagctgccg gtgacgatca
     7441 ccaaggacat catggccgga gtgatcgccg tgccgcacgg ctggggccat aagggcaccg
     7501 gcggctggcg ggtcgccaac gctgccggcg gcgcgaacgt caaccgcctg atgtccagcg
     7561 cgccggaaga cctggaacgg ctggccggga tggctcattt gaccggcgta cccgtgcgag
     7621 tcgatcgcgc gtagcgtcgc ggcgccgcgt ggttgcatag ggctatggcc caaggactcg
     7681 gtcgtctcca cggaaagtcg gctgtgatca ccggcgcggc gttcggcatc ggtcgggcga
     7741 ccgccgagct gttcgtgcgc gagggcgcgc gcctgaccgc gaccgatatt caaggcgaac
     7801 cgctgctggc cctcgccgat gaattgcggc agggcggggc ggaggtcgag acggtcgtcg
     7861 gcgacgtctc gatcgaggcg gacgcgcgac ggatgatcgg ggccgcgacc gaccgattcg
     7921 ggcggcttga catcttggtc gccaacgccg gcatcatccc cctcggcgac gcgacggaaa
     7981 tgaccgccgc cgactgggac aacgtaatgg ccattgacgg gcgcggcatg ttcctcacct
     8041 gcaagttcgc gatcgacgcg atggtgccga ccggcggtgg cgccatcgtc tgcctgtcct
     8101 cgatctccgg cctggccggg cagaagcggc aggcggtgta cggccccgcc aagttcgtcg
     8161 ccaccgggct gaccaagcac ttggcggtcg agtgggccga ccgcggaatc agggtcaacg
     8221 ccgttgcccc cggaacgatc cgcacagagc gggtcaagca gttgccggac gagccgggcg
     8281 gctcagaata cctcgcggcg atcgagcaga tgcacccgat gggccgcatc ggcgaaccgg
     8341 ccgaagtcgc cagcgccatc gtcttcctcg cctctgacga cgcctctttc atcaccggtg
     8401 cagtgttacc ggtcgatggg ggataccttg cgcaatagca gttaaacccg ctgcgcaact
     8461 tcggcattga ccgcttggtc gaggaaatcg ggtggcacct cgggccattc gggacgccaa
     8521 caaccggacc gctgatcggc ctgcagttcc gaaagtattg cggcgacata cggaccacca
     8581 tcgacgagat aacgcagcag ccccaactgt gcacattgcg ccgccacgga cgcgattgtg
     8641 ctctttgcct cgtcgacgcg tccggcggcc gacaggctcg cacccagcag acggcgcgcc
     8701 cggagcagcg cctgcgggcg gttcggcgat gcggttcgat ccacccactc ctgcgcccac
     8761 ctacaggcaa gctgtttctt ctctggatcg tcggccgcca tcagcagccg gatggccgat
     8821 gcctcctcga attggaccgt gatctcgtcg atcgcgtcca ccggctggcg gcgtgactcg
     8881 taagacgccg gcggccacat atcggaatcc ggatgcggtg gaagcccgag ccgggtgcgt
     8941 tcgtgctccg ccatcgcccg caggcggttc agggatagtg accgcgcgac ccggatggcc
     9001 tcgtccaggc gttgaccggc agcaagccgg tcgccctgca gcgccttgac tctcgcggcg
     9061 atgacatacc gagcgatctt gaagtcgacc gagccgccct caggcccgag cttgtagccc
     9121 tcctccagca ggcgggcggc ctcgtcgaga tcgcctcgct cgtaaagcaa ttcgcccagt
     9181 gccgagcttg ccaaccgggc ggtataggaa tgactgccgc cggatcgctt ggcgatcttg
     9241 agggccttgc ggaagcaggc ttccgccgtt gtgatgtcga gcatcaactg gtgcgcgagg
     9301 cccgtgtagc acagtccgtg aaccgtattg aaggtgtccc ggtttcgttc ggcgtatggc
     9361 gctgcccacg cctggatccg gtttacctcg tcgaggttgt agtggtacgc ggccgcgaac
     9421 gttgccacat tggcggccac cccaacggtg aacgggcgca ggtgttctcg gcgcgccaga
     9481 cacggtgcga tgtgttcgtc gatgcccgcg agccggtcgg cgcgcagttc cgcaacgccg
     9541 cgcacaactc cgccgtccgc gcgcaggtcg gcaatctcgt cgtcgccgag gccgcagcgt
     9601 tccagccctg attccatacg ggccaatgct tgttcggctg cgggaattct gtgcaagacg
     9661 atgttggccc aggccagggc gagttgcagc cgcggatccg tcttcacgac ggcggccggc
     9721 agcttgccca ccagaccgat cagcgtggcc atttgcgaat tcgccaccag atcgacgccg
     9781 tgattctcga cgagctggac cgccctctcg tcgtcaccgg ccgccagcgc gtgatcgacc
     9841 gcctcactga cgaaatggtt gtcggcgaac cacgtcgagg cggcgcggtg gagccggacg
     9901 acccgctggg gttcgtcacg gcccagccgg tgcagcaaga agtcgaggaa cagttggtgg
     9961 tagcggaacc actgctcgtc gacccggcgc aggaacaggt cgcgttcctc gacctgctcg
    10021 agcatcgcct gcccgtccgg cacgccggac aacgccgtcg ccaggtcgcc acagatccgc
    10081 tcggtgatcg aggtggccag caggaagtcg agcatcgacg gttccagcgt gtcgagcaca
    10141 ttctccgcga ggaactcgct gatcgcgtga tgtcggccgg tcatcgtgcc gatcaggcgt
    10201 tcaggatcgt cgcggccgcg cagcgatagc gatgccaact gcagtgcggc cacccagccg
    10261 tccgtcttgg cggtgagctc ctcgacgtcg gggcggtcca ggtccagccc actcaggtca
    10321 accagaaagt tctccgattc ggcgacgtcg aaacgcaatg cagtggcatc gatttcgaga
    10381 agttcgtcct gcatccgcat ccggctcatc ggcaggccgc tctggttgcg acttgtcacc
    10441 accacggtca aatcgtcaga ggcgtatacg agtaggtaca gcagcgcggc gatggtcgcc
    10501 gggtcggtga cgcgctgcca gtcgtcgatc accagcgtca tgcgggtgcc cgtctcatgg
    10561 atctcgttga tcaacgaggt caggacgtaa cgctcggcct cgtcgtcgtg ctcctgcaac
    10621 acttcgccga gctcgg
//
LOCUS       DASRJA010000362         6431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_40818,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000362 DASRJA010000000
VERSION     DASRJA010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40818"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFC01_16125"
     CDS             <1..231
                     /locus_tag="VFC01_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81193.1"
                     /translation="QWPLFAGFCFYAYYKFVRYEDAPPEPRNHDAVTEIPSGILPERP
                     KPATPSDDDTTLREYNAYLAELAKQDKEHPTL"
     gene            228..563
                     /locus_tag="VFC01_16130"
     CDS             228..563
                     /locus_tag="VFC01_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HZN81194.1"
                     /translation="MTEAPTTPVETIRKALLGYRVMAWTTGIWLIALCYEMVLKYIVQ
                     VDNPPNWIAIVHGWVYFVYLIFTANLAVKVRWPIGKTIGVLLAGTVPLVGIIVEHFQT
                     ADIKQKFGL"
     gene            complement(541..1155)
                     /gene="rdgB"
                     /locus_tag="VFC01_16135"
     CDS             complement(541..1155)
                     /gene="rdgB"
                     /locus_tag="VFC01_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZN81195.1"
                     /translation="MTELLVASRNRKKLAELRRVLDGAGVALTLLSLDDVAPFDEAPE
                     TGATFEENALAKARDAFRATGLASVADDSGLEVDALNGMPGVLSARWSGRHGEDSANT
                     ALLLGQLQDVPDSRRGAAFVSACALVSDGGEVVVRGVWPGSIVREARGDGGFGYDPVF
                     LPAGSDRTAGELTPEEKDAVSHRGRALGALLPALRELATGRTSA"
     gene            complement(1152..1934)
                     /gene="rph"
                     /locus_tag="VFC01_16140"
     CDS             complement(1152..1934)
                     /gene="rph"
                     /locus_tag="VFC01_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZN81196.1"
                     /translation="MSRREDGRLDDELRPVRITRGFTSHPAGSVLVEFGETRVMCTAS
                     VNEGVPRWRKGSGQGWLTAEYAMLPAATHERSDRESVKGRVGGRTQEISRLVGRSLRA
                     CIDLAALGENTIAIDCDVLQADGGTRTAAITGAYVALADAVTYLSAAGRLSDPRPLSC
                     AIAAVSVGVVDGRIRVDLPYSEDSRAEVDMNVVATDTGTLVEIQGTGEGATFPRSTLD
                     KMLDAAMAACEELFAIQREALELPYPGVLPESGEPSKKAFGS"
     gene            complement(1967..2740)
                     /locus_tag="VFC01_16145"
     CDS             complement(1967..2740)
                     /locus_tag="VFC01_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-degrading phosphodiesterase"
                     /protein_id="HZN81197.1"
                     /translation="MSVRITVLGCSGSVVGPDSPASGYLLTAPDTPPLVLDFGGGVLG
                     ALQRHADPNSVHVLLSHLHADHCLDLPGLFVWRRYHPTPADERGLMYGPANTWARLGA
                     ASSPEGGEIDDFSDIFDIRHWADNEAVQIGSLSVVPKLVCHPTESYGLRISDPSGATL
                     VYSGDTGYCDALIDLARDADVFLCEASWTHSPSRPPKLHLSGTEAGRAAAQAGVGELL
                     LTHIPPWTSREDVISEAKAEFDGPVHAVVCNEAFDITRS"
     gene            complement(2799..3623)
                     /gene="murI"
                     /locus_tag="VFC01_16150"
     CDS             complement(2799..3623)
                     /gene="murI"
                     /locus_tag="VFC01_16150"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZN81198.1"
                     /translation="MNSALAPVGIFDSGVGGLTVARAIIDQLPDEDIVYVGDTANGPY
                     GPLTVPEIRAHALAIGDDLVARGVKALVIACNTASSAMLRDARERYAPVPVIEVVLPA
                     VRRAVAATRNGRIGVIGTAATIASGAYQDAFAAARDTEVIGVACPRFVDFVERGVTSG
                     RQVLGLAEGYLEPLQRAEVDTLVLGCTHYPMLSGLIQLAMGNHVTLVSSAEETAKDLL
                     RVLTELDLLRPHDAPQARRVFESTGDPEAFTTLAARFLGPTLDGVRPVQRHVAAQR"
     gene            complement(3620..4306)
                     /locus_tag="VFC01_16155"
     CDS             complement(3620..4306)
                     /locus_tag="VFC01_16155"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZN81199.1"
                     /translation="MGLTGPGYQATPAEPKKRPAWIVGGVTILTFVALLWVIELFDSL
                     SNHRLDDNGIRPLETDGLWGIIFGPLLHSNWDHLIANTIPALILGFLMTLAGLSRFIF
                     ATAIIWILGGLGTWLIGNVGAHCPYVGVRCETNHIGASGLIFGWLAFLIVFGFFTRKV
                     WEIVVGVIVLFVYGGVLLGVLPGTPGVSWQGHLCGAIAGVLAAYLLSGPERKAREARK
                     RKATNPYLTS"
     gene            complement(4297..5259)
                     /locus_tag="VFC01_16160"
     CDS             complement(4297..5259)
                     /locus_tag="VFC01_16160"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HZN81200.1"
                     /translation="MTRYDSLLEALGNTPLVALHRLSPRWDDEPHVRLWAKLEDRNPT
                     GSIKDRPALRMIEDAERRGLLQPGATILEPTSGNTGISLAMAALLKGYQMICVMPENT
                     SIERRQLLELYGAKIIYSPAEGGSNTAVAHAKELAAENPSWVMLYQYGNESNTLSHYE
                     GTGPELLADLPEITHFVAGLGTTGTLMGTGRYLRENAPGVQIVAAEPRYGEGVYALRN
                     IDEGFIPELYDPDVLTTRYSVGSYDAIKRTRELVQVEGIFAGISTGAVLHAALGMAAK
                     AVKAGERADIAFVVADAGWKYLSTGAYAGSLDDAEDALEGQLWA"
     gene            complement(5261..5533)
                     /locus_tag="VFC01_16165"
     CDS             complement(5261..5533)
                     /locus_tag="VFC01_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZN81201.1"
                     /translation="MSVTVSIPTILRTHTGGEKRVSATGATLQAVISDLEANYSGISE
                     RLVDNGKLHRFVNIYVNDEDVRFSGGLDTEISDGDSVTILPAVAGG"
     gene            complement(5546..5959)
                     /locus_tag="VFC01_16170"
     CDS             complement(5546..5959)
                     /locus_tag="VFC01_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HZN81202.1"
                     /translation="MLVIRADLVDAMVAHARADHPDEACGVIAGPEGSDRPERFIAML
                     NAERSPTFYRFDSGEQLKVWREMDEADEVPVVIYHSHTATEAYPSRTDISYASEPDAH
                     YVLVSTRDPDELELRSYRILDGAVTEEPVKIVEQY"
     gene            complement(5973..>6431)
                     /locus_tag="VFC01_16175"
     CDS             complement(5973..>6431)
                     /locus_tag="VFC01_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HZN81203.1"
                     /translation="VDTDTGLPWLAYQIEEFGLVPPPPDQIAAYAARRAEFSPLNTTI
                     AVVATDAALSPAGCRRVAVAAHSGLSRTIRPCHTPLDGDTVFALATGAIEVPPDPTTP
                     ASMSPETPLITDVGAAAADCLARAVLVGVLAAESVAGIPSYRDMLPGAFE"
BASE COUNT         1178 a         2272 c         1976 g         1005 t
ORIGIN      
        1 caatggccgc tgttcgccgg gttctgcttc tatgcctact acaagttcgt ccgctacgag
       61 gacgccccgc ccgagcctcg aaaccacgac gccgtgaccg agatcccttc cgggatactg
      121 cccgaaagac ccaagcccgc aacaccttct gacgacgaca caacactccg cgagtacaac
      181 gcctatctcg ccgaactggc caagcaagac aaggaacacc cgaccctatg accgaagccc
      241 cgaccacccc cgtcgaaacc attcgcaagg ctctgctcgg ttaccgcgtg atggcatgga
      301 cgaccggcat ctggttgatc gccctctgct acgagatggt gctgaagtac atcgtgcagg
      361 tcgacaaccc gccgaactgg atcgcgatcg tgcacggctg ggtctacttc gtctacctga
      421 tcttcaccgc caacctcgcc gtcaaggtgc gctggccgat cggcaagacc atcggcgtgc
      481 tgctggccgg caccgtcccg ctcgtcggca tcatcgtcga gcacttccag accgccgaca
      541 tcaagcagaa gttcggcctg tagccaactc ccgcagcgcc ggaagcaagg ctcccagcgc
      601 acgtccacga tgcgacaccg cgtccttctc ctcgggcgtc aactcccccg ccgtcctgtc
      661 cgaccccgcc ggcaggaaga ccgggtcata gccgaagccg ccgtcgcccc gcgcctcccg
      721 cacgatgctg cccggccaca ccccgcgcac cacgacctcg ccgccgtctg acaccaacgc
      781 acacgccgac acgaacgccg cgccgcgccg cgagtccggc acgtcctgaa gctgtcccaa
      841 cagcagcgcg gtgttggccg aatcctcgcc gtgccgcccc gaccaccgcg ccgacaacac
      901 gcccggcatc ccgttcagcg catccacttc cagcccggaa tcgtcagcca cagaagccaa
      961 cccggtagcc cgaaacgcgt cgcgcgcctt cgccaacgcg ttctcctcga acgtcgcacc
     1021 cgtctccggc gcctcgtcga acggcgcgac gtcgtccaac gacaacagcg tcaacgcgac
     1081 cccggcacca tcgagcaccc ggcgcaattc ggccagcttc ttgcggttgc ggctggccac
     1141 caacagctcg gtcagcttcc gaacgccttc ttcgaaggtt cccccgactc gggcagcacc
     1201 ccgggatacg gcaactccaa cgcctcgcgc tggatcgcga acaactcctc gcaggcggcc
     1261 atcgccgcat ccagcatctt gtccagtgtc gagcgcggga acgtcgcacc ctcacccgtg
     1321 ccctggatct cgaccagcgt gccggtgtcg gtcgcgacga cgttcatgtc cacttcggcg
     1381 cgcgagtcct ccgaatacgg caggtcgacc cggatgcggc cgtcgacgac accgacgctc
     1441 accgccgcga tcgcacatga cagcggccgc gggtccgaca gccgaccggc cgccgacagg
     1501 tacgtcaccg catcggcgag cgctacatac gcgccagtga tcgccgccgt gcgggtaccg
     1561 ccgtcggcct gcagcacgtc gcagtcgatc gcaattgtgt tctcccccaa cgccgctagg
     1621 tcgatgcacg cacgcagcga ccggcccaca aggcggctga tctcctgcgt gcgcccgccg
     1681 acgcggccct tgaccgactc gcgatccgac cgctcatggg tggccgcggg aagcatcgca
     1741 tactcggccg tcaaccagcc ctggcccgaa cccttacgcc accgcggcac accctcattg
     1801 acgctcgcgg tgcacatgac cctggtttcg ccgaactcca ccaacaccga tccggcggga
     1861 tgcgacgtga agccacgcgt tattcggacc gggcgaagct cgtcgtcgag ccggccgtct
     1921 tctcgtctgg acacgcgcca accctaaccg gtgggtctga ccgctctcag gaacgtgtga
     1981 tgtcgaaggc ctcgttgcac accactgcgt gcacgggacc gtcgaactcc gccttcgcct
     2041 cgctgatgac gtcctcgcgt gacgtccacg gcggaatgtg ggtcaacagc agctcgccga
     2101 cgcccgcctg cgcggctgcg cgtcccgcct cggtaccgga cagatgcaac ttgggcggcc
     2161 gcgacggcga atgcgtccac gacgcctcgc acaagaacac gtcggcgtcg cgggccagat
     2221 cgatcaacgc atcgcaatag ccggtgtcgc cgctgtacac cagcgtcgcg cccgacggat
     2281 cggagatccg cagaccgtag gactcggtcg gatgacacac gagtttcggt accaccgaaa
     2341 gcgaaccgat ctgcaccgct tcgttgtccg cccaatgccg gatgtcgaag atgtcggaga
     2401 agtcgtcgat ctccccaccc tcgggcgacg acgctgcgcc caaccgcgcc cacgtgttcg
     2461 ccgggccgta catcaaaccg cgctcgtcag ccggcgtcgg gtggtagcgc cgccacacga
     2521 acaggccggg cagatcgaga cagtgatcgg cgtgcaggtg cgagagcagc acatgaaccg
     2581 agttggggtc ggcatgacgc tgcagcgcgc cgagtactcc acccccgaaa tcgaggacca
     2641 acggaggggt gtcaggggcc gtcaacagat agcccgaagc tggcgaatca ggcccgacga
     2701 cactgccgga gcaaccgagt acggtgattc gcacgctcac tagcttgcca tgcccgctat
     2761 ccgtgtgacg aaaacatcgc ccgtttacgc gacggaaatc atctttgagc cgcaacgtga
     2821 cgctgaacag gccgaacacc atccagcgtc gggcccagga aacgggccgc aagtgtggtg
     2881 aacgcctcag gatccccggt cgactcgaac accctccgcg cctgcggagc gtcgtgggga
     2941 cgcaacaaat caagctcggt gagcaccctc agcaagtcct tggcggtctc ctcggcgctg
     3001 gacaccaacg tcacgtggtt gcccatcgcc agctggatca gccccgagag catcgggtag
     3061 tgggtgcagc cgagcaccag cgtgtcgacc tcggcacgct gcagcggttc caaatagccc
     3121 tccgcgagtc caagcacctg gcggccgctc gtcacgccgc gctcgacgaa gtcgacgaac
     3181 cgcgggcagg ccacgccgat cacctcggtg tcgcgcgcgg ctgcgaacgc atcctgatag
     3241 gcacccgacg cgatggtcgc cgcggtgccg atcacgccga tccggccgtt gcgcgtcgca
     3301 gccacggccc gtcgcaccgc gggcaggacc acctcgatga cgggcacggg tgcatatcgc
     3361 tcgcgcgcgt cgcgcagcat cgccgacgac gcggtgttgc atgcgatcac tagcgccttg
     3421 acgccgcgcg cgacgaggtc gtcgccgatt gccaaagcgt gcgcacggat ctcgggaacg
     3481 gtcagcgggc cgtacggacc attcgcggtg tcgccgacgt agacgatgtc ctcgtctggc
     3541 agctggtcga tgatcgcccg cgcgacggtc aggccaccga cgccggaatc gaaaatcccg
     3601 acgggcgcca atgcagaatt cacgacgtca gatatgggtt cgtcgctttg cgtttacgtg
     3661 cttcgcgcgc cttgcgttcg ggcccggaca gcaggtacgc ggcgagcacg cccgcgatcg
     3721 caccgcacag gtgcccctgc cacgagaccc cgggcgtgcc gggcagcacg ccgagcagga
     3781 cgccgccgta gacgaacaac acgatgacgc cgaccacgat ctcccacacc ttgcgggtga
     3841 agaacccgaa cacgatcagg aacgccagcc agccaaagat gaggcccgac gcgccgatgt
     3901 ggttggtctc gcagcgcacg ccgacgtagg ggcagtgcgc acccacgttg ccgatcagcc
     3961 aggtgcccaa cccgccgagg atccagatga tcgcggtggc gaagatgaac cgcgacaacc
     4021 ccgccaacgt catcaggaag ccgaggatca gtgccggaat ggtgtttgcg atcaggtgat
     4081 cccagttcga atgcagcagc ggcccgaaga tgatgcccca cagcccgtcg gtctccaggg
     4141 gccggatgcc gttgtcgtcg aggcggtgat tggacaacga gtcgaacagc tcgatgaccc
     4201 acagcagcgc gacgaacgtg aggatcgtga ccccgccgac gatccacgcc ggccgcttct
     4261 tcggctccgc cggcgtcgcc tgatatccgg gacctgtcaa gcccatagct gcccttccaa
     4321 tgcgtcttcc gcatcatcca ggctaccggc gtaggcaccg gttgacagat acttccaacc
     4381 ggcgtcggcg acgacgaacg cgatgtccgc acgctcaccc gccttgaccg ccttcgccgc
     4441 catgcccagg gccgcatgta acaccgcgcc ggtggagatc ccggcgaaga taccttcgac
     4501 ctgcaccagc tcgcgcgtgc gtttgatcgc atcgtaggaa ccgacggagt agcgggtggt
     4561 cagcacgtcg gggtcataga gctcggggat gaagccctcg tcgatgttgc gcagcgcgta
     4621 aacaccttcg ccgtaacggg gttcggccgc gacgatctgc acgccgggcg cattctcacg
     4681 cagataccgc ccagttccca tcaatgttcc tgtggtgccg agacccgcaa cgaaatgggt
     4741 gatttcgggc aggtcggcca agagttcagg tcccgtgccc tcgtaatgcg acaaggtgtt
     4801 ggactcgttg ccgtactggt agagcatcac ccacgaaggg ttttcggccg caagctcttt
     4861 cgcgtgcgcc accgcggtgt tcgatccgcc ctcggccggc gagtagatga tcttcgcgcc
     4921 gtagagctcg agcagttgtc ggcgttcgat cgacgtgttc tccggcatca cgcagatcat
     4981 ctgatagccc ttgagcagcg cagccattgc caacgagatt ccggtgttgc cgctcgtcgg
     5041 ctcgaggatc gtcgcaccgg gctgcaggag gccacggcgc tcggcgtcct cgatcatgcg
     5101 cagcgcaggc cggtccttga tggacccggt cggattgcgg tcttcgagct tggcccagag
     5161 ccgcacgtgc ggttcgtcgt cccagcgggg cgaaagccgg tgcagcgcaa ccaaaggcgt
     5221 gttgcccagc gcctcaagca gcgaatcgta acgcgtcaac ctaaccgcct gcgacggccg
     5281 gcaggatcgt caccgagtcg ccgtcggaga tctcggtgtc cagcccgccg gagaaccgca
     5341 cgtcctcgtc gttgacgtag atgttcacaa agcggtgcaa cttgccgttg tcgaccaacc
     5401 gctcggagat gcccgagtaa ttcgcctcca ggtcgctgat gacggcctga agcgtggcgc
     5461 cggtcgcgga gacccgcttc tcaccgccgg tgtgggtgcg caggatggtc gggatcgaca
     5521 cagtgacgga catgaatagg cctttctaat actgctcaac gattttgacg ggttcctcgg
     5581 tcacggcgcc gtcgaggatg cgatagctgc gcagctctag ctcgtcggga tcgcgggtgg
     5641 acaccaacac gtaatgggca tcgggttcgg acgcatatga aatgtcggtc cggcttgggt
     5701 aggcctcggt cgcggtatgc gagtgataga tgacgacagg cacttcgtcg gcctcatcca
     5761 tctcgcgcca caccttgagc tgctcgcctg agtcgaaccg gtagaaggtc ggcgaccgtt
     5821 cggcgttgag catcgcgatg aaccgctcgg ggcgatcaga accctcgggg cccgcgatca
     5881 ccccgcacgc ctcatccgga tggtcggccc gagcatgcgc caccatggcg tcgaccaggt
     5941 cggctcggat caccaacaca gctgcttctc cctcattcga acgctcccgg caacatgtcc
     6001 cggtacgacg gtattccggc gaccgactca gcggccaata cgccgaccag caccgcacgg
     6061 gccaggcagt ccgccgcagc cgcgccgacg tcggtgatca gtggtgtctc cggcgacatc
     6121 gacgcgggcg tcgtggggtc cggcggcacc tcgatggcac ctgtggccag cgcgaacacc
     6181 gtatcgccat ccagcggagt gtggcacggg cggatggtgc gggacagacc gctgtgcgcg
     6241 gcgacggcga cccggcggca gcccgccggg ctcagcgcgg cgtcggtggc caccaccgcg
     6301 atcgtggtgt tgagcgggct gaactcggcg cgacgcgcgg cataagcggc gatctggtcg
     6361 ggtggcggag gtacgagacc gaactcctcg atctggtatg ccagccacgg cagcccggtg
     6421 tccgtgtcga c
//
LOCUS       DASRJA010000363         3449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2201477,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000363 DASRJA010000000
VERSION     DASRJA010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2201477"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /gene="glgX"
                     /locus_tag="VFC01_16180"
     CDS             complement(<1..1249)
                     /gene="glgX"
                     /locus_tag="VFC01_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HZN81204.1"
                     /translation="MSNGAVPEIWPGKAYPLGATYDGSGTNFAVFSEAADRVELCLFD
                     NGTETRVTLPEVDGFVWHGFIPNIEPGQRYGYRVYGPYDPAAGQRCNPNKLLIDPYAK
                     AIDGTFEWNQSLFGYNFGDPDSRNDDDSAASMPKCVVISPYFDWGVDRPPQHEYADTV
                     IYEAHVKGLTQTHPDIPENIRGTYAAVAHPVIIEHLKSLGVNAIELMPVHHFVNDSTL
                     VEKGLTNYWGYNTIGFLAPDAKYSSSPNPGGQVQEFKAMVRTLHEADIEVILDVVYNH
                     TAEGNHLGPTLSMRGIDNGAYYRLVDDDKRYYLDYTGTGNSLNVGHPHSLQLIMDSLR
                     YWVTEMHVDGFRFDLAATLAREFYDVDRLSAFFELVQQDPTVSQVKLIAEPWDVGEGG
                     YQVGNFPPLWSEWNGKYRDVVRDF"
     gene            1275..2072
                     /locus_tag="VFC01_16185"
     CDS             1275..2072
                     /locus_tag="VFC01_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN81205.1"
                     /translation="MPAYRTLLFDVDCGVASITLNRPQQRNAIGDGMRDELADAFRTC
                     DRDDSVRVIVLTGTPPAFCAGADLAGGEQTFAEPGPSFSAAGFAVPAWSLSKPVIAAV
                     NGHAIGLGLTLALQCDIRFFAADARYGVVQVRRGVVGDAYSHWVLPRLVGIANAAEIL
                     LTGATFDGHRAVQLGLGSRVLDAADVLPAALELAHDIAQNTAPMSVAASKRLLWDSFD
                     LDREAVGARETEIHRHLMRHDDAKEGVRAHVEGRPPRWTGPPRDPTD"
     gene            complement(2069..2620)
                     /locus_tag="VFC01_16190"
     CDS             complement(2069..2620)
                     /locus_tag="VFC01_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HZN81206.1"
                     /translation="MRALIIVDVQNDFCEGGSLGIAGGTAVVREINEYVAAHRDYAHV
                     VATKDYHIEPGEHFSEHPDYRVSWPRHCEVGTPGVDFHPEFDDSAIEAVFMKGHYSAA
                     YSAFESVDDTGTPLPDWLRQRGVEEIDIAGLATDYCVLATATDAVNAGFAARVLLDLT
                     AGVAQESTDKAVEDMRAAGVEIA"
     gene            complement(2630..>3449)
                     /locus_tag="VFC01_16195"
     CDS             complement(2630..>3449)
                     /locus_tag="VFC01_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81207.1"
                     /translation="GLFAFVWLELASPDPGSLGAIKVWLLVYLVVTLAGALCCGARWC
                     ARADPFEVYSVVASRLSVLRRNRDGRIAIGNPFDHLPSLPVRAGTLAVLAVLLGSTAF
                     DSFSATPQWRGFVDDHTDSVATATLLRTAGLVVFAGVVAGTFWLAARTAGGVDAQRRR
                     ELPGLMAHSLIPIVIGYVFAHYLTYLVERGQQTVIQLFSLGTDVHYVLSMHPSLLASI
                     KVSCVVAGHIAAVIAAHDRALRLLPAGHQLTGQLAMMLVMVGYTFTGLYLLFGG"
BASE COUNT          595 a         1151 c         1127 g          576 t
ORIGIN      
        1 agaagtcccg cacgacgtcg cggtacttgc cgttccactc cgaccacagc ggcgggaaat
       61 tgccgacctg gtacccgcct tcgccgacgt cccacggttc ggcgatcagc ttgacctggc
      121 tgaccgtcgg atcctgttgc acgagttcga aaaacgcaga cagccggtcg acgtcgtaga
      181 actcgcgcgc cagggtggct gcgaggtcga agcggaagcc gtcgacgtgc atctcggtca
      241 cccagtaccg cagcgaatcc atgatcagct gcagcgaatg cggatggccg acattgaggc
      301 tgttacccgt gccggtgtag tccaggtagt accgcttgtc gtcatcgacc agacggtagt
      361 aggcgccgtt gtcgataccg cgcatcgaca acgtcggtcc caggtggttg ccctcggcgg
      421 tgtggttgta aaccacgtcg agaatcacct cgatatcggc ctcgtgcagc gtccgcacca
      481 tggccttgaa ctcctggacc tgcccgcccg ggttcgggct ggagctgtac ttcgcgtccg
      541 gcgccaaaaa tccgatcgtg ttgtagcccc agtagttcgt cagccccttc tcgacgagcg
      601 tcgagtcgtt gacgaagtga tgcaccggca tcaactcgat cgcgttgacg cccaacgact
      661 tcaagtgctc gatgatgact ggatgcgcga ctgcggcata tgtgccgcga atgttctccg
      721 gaatgtccgg atgggtctgg gtaaggccct tgacgtgcgc ctcgtagatc acggtgtcgg
      781 cgtattcgtg ctggggcggt cgatccaccc cccagtcgaa gtacgggctg atgaccacgc
      841 acttgggcat gctggccgcc gagtcgtcgt cgttgcggct gtcgggatcg ccgaagttgt
      901 atccgaacag cgactggttc cactcgaagg tgccgtcgat cgccttcgcg tacgggtcga
      961 tcagcaactt gttcgggttg cagcgctgtc ccgcggcagg atcgtatggg ccgtacaccc
     1021 ggtagccgta gcgctggccg ggctcgatgt tcgggatgaa cccgtgccag acgaaaccgt
     1081 cgacttccgg cagcgtgacg cgcgtctcgg tgccgttgtc gaacaggcac aattcgacgc
     1141 ggtcggcggc ctcgctgaac accgcgaaat tggttcccga accgtcgtag gtggcgccca
     1201 gcggatacgc cttgcccggc cagatttccg gcaccgcgcc attactcact acggcgacga
     1261 tacggtgtcc gtcgatgccc gcttaccgca ccttgttgtt cgacgtcgat tgcggcgtgg
     1321 cctcaatcac actcaaccgg ccacagcaac gcaacgcgat cggcgacggg atgcgcgacg
     1381 agctggccga cgccttccgg acgtgcgatc gcgacgactc ggtgcgggtg atcgtgctga
     1441 ccggcacccc gcccgcgttc tgtgccggcg cggacctcgc cggcggtgag cagaccttcg
     1501 ccgaacccgg accgagtttc agtgcggccg gcttcgcggt gcccgcatgg tcgctgtcca
     1561 agccggtgat cgcggccgtc aacggacacg ccatcggcct cggcctgacg ctggcgctgc
     1621 aatgcgacat ccggttcttc gccgccgacg cccggtacgg cgtggtgcag gtccgccgcg
     1681 gcgtcgtcgg tgatgcctac tcgcattggg tgctgccgcg gctggtcggg atcgccaatg
     1741 cggccgaaat cctgttgact ggagcaactt tcgacggcca tcgcgcagtg cagctgggtt
     1801 tgggcagccg ggtgctcgac gccgccgatg tgctgcctgc cgcgctggag ctggcccacg
     1861 acatcgcgca gaacaccgcg ccgatgtcgg tggcggccag caagcggttg ctgtgggatt
     1921 cgttcgacct cgatcgcgag gccgtcggcg cccgcgagac cgagatccac cgccacctca
     1981 tgcgtcacga cgacgccaaa gaaggcgtgc gcgcgcacgt ggaaggccgc ccgccccggt
     2041 ggacgggccc gccgcgcgac ccgaccgact aggcgatctc gacgccggcg gcgcgcatgt
     2101 cctcgactgc cttgtcggtt gactcctgtg ccacgccggc ggtcaggtcg agcaggaccc
     2161 gtgcagcgaa accggcgttg acggcgtcgg tggcggtcgc cagcacgcaa tagtcggtgg
     2221 ccaatccggc gatgtcgatc tcctccacgc cgcgctgccg caaccagtcg ggcagcggcg
     2281 tgccggtgtc gtcgacggat tcgaacgcgc tgtaggcggc tgagtagtgg cccttcatga
     2341 acaccgcctc gatggcgctg tcgtcgaact ccgggtgaaa gtcgacgcct ggggtgccga
     2401 cctcacaatg ccgtggccag gacacccggt aatcggggtg ctcagagaaa tgttcgccgg
     2461 gctcgatgtg gtagtccttg gtggcgacca cgtgggcgta gtcgcggtgg gcggcgacgt
     2521 actcgttgat ttcgcggacc accgctgtgc caccggcaat ccctagggaa ccgccctcgc
     2581 agaaatcgtt ctggacgtcg acgatgatca gtgcgcgcat gcctcaacgt tagccgccga
     2641 acaacagata gagcccggtg aacgtgtagc cgaccatcac cagcatcatc gccagctgcc
     2701 cggtcagctg atggccggcg ggcagcaacc gcaatgcccg gtcgtgggcc gcgatcaccg
     2761 ccgcgatgtg accggccacc acacaactca ccttgatgct ggccagcagc gacgggtgca
     2821 tggacagcac gtagtgcacg tcggtgccaa ggctgaacag ctggatgacc gtttgctgcc
     2881 cacgttcgac gagatacgtc agatagtgcg cgaacacgta gccgatgacg atcgggatca
     2941 gcgaatgcgc catcagcccg ggcagttcgc ggcgacgctg cgcatcgacg ccgcccgccg
     3001 tccgcgcagc gagccagaag gtgcccgcga ccaccccggc gaacacaacc agccccgcgg
     3061 tgcgaagcaa tgtcgccgtc gcgaccgagt ccgtgtgatc gtcgacgaag ccgcgccact
     3121 gcggcgtcgc cgaaaagctg tcgaacgccg tcgatcccag cagcaccgcc agcaccgcca
     3181 gcgtgccggc ccgcaccggc agtgacggca ggtggtcgaa cgggttgccg atcgcgatgc
     3241 gaccgtcgcg gttgcggcgc agcaccgaaa gccgagacgc caccacgctg tacacctcga
     3301 acggatcggc gcgggcacac caccgcgcgc cgcagcacag tgcgccggcc aacgtcacga
     3361 cgaggtacac gagcagccaa accttgatcg cgccaagcga tcccggatct gggctggcga
     3421 gctcgagcca gacgaacgcg aacagcccc
//
LOCUS       DASRJA010000364         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1132634,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000364 DASRJA010000000
VERSION     DASRJA010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132634"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="VFC01_16200"
     CDS             <1..1606
                     /locus_tag="VFC01_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl reductase"
                     /protein_id="HZN81208.1"
                     /translation="GGYTQADVTEARYVLYVADPAADPAETDIDLAVRLSSEVADVVR
                     QLAERDYRHPATLWIVTRGVREADSEAALRQSCLWGLAGVIGAEQPQLWGGLIDIAVG
                     DDIADCAAALSAVLPTPAKSVLSLRDGEFFETALAPISGEPVREPVRCRPDAAYLITG
                     GMGALGLLMAGWLADRGARRLILAGRTPLPPRREWDGDITDTDVHDKIDAIRALEMRG
                     VSVDTVALDIGSREAVQALLARRDSDGAPPIRGVIHAAGVTEGQLLTEVSDSRLHRTM
                     WPKIAGAQSLHEAFPPASLDFFFLTASAGTVFGIPGQGAYAAANSYLDCLARARHRQG
                     CNTVSLDWVAWQGLGFGSDAQIVIQELERVGSRPVTPYEAFTAWEHVTCYDVAQAVMA
                     PMPSAEETAIGISNAHRPSAPVRAWSQMTSEDLRAELQNGLRTILATELRIPIDEVEL
                     DRPFAEMGLNSVMAMSIRREVEQLVGIELSATMLWNHPTIASFSGYLAEQLVPQESSD
                     GDSEIDGSTDSVLDSLFDSVESESRI"
     gene            1659..>2522
                     /locus_tag="VFC01_16205"
     CDS             1659..>2522
                     /locus_tag="VFC01_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN81209.1"
                     /translation="MLADRAQKQPDDIAYTFIDYEVDLAGYAESLTWAEVHHRARVVA
                     QKLATCGSPGDRAVILAPQSLEYIIAFLGAIQAGFVAVPLSVPQFGQHDERVSGALGD
                     STPAAILTTSAVVDDVRKYAQAQPRQRAPRVVEIDALDFDSPISDAALVPISKPAYLQ
                     YTSGSTRQPAGVMVTHQNILTNIEQLQPDYFERSGGISPQDMTIVSWLPLYHDMGLLV
                     GLFLGMAFGHPAVLMSPVAFMQKPARWMQQLAMNSSTFTAAPNFAYDLAVRRTSDDDL
                     AGMDLGGVQVMI"
BASE COUNT          456 a          783 c          819 g          464 t
ORIGIN      
        1 cggcggctac acgcaggccg acgtcacgga ggcacggtat gtcctctacg tagccgaccc
       61 tgccgcggac ccggctgaaa ccgacatcga tttggcggtt cgcctgtcat ctgaagttgc
      121 cgacgtcgtc cgccagctgg ccgagcggga ttaccgccac ccggccacgc tctggatcgt
      181 cacccgcggt gtccgcgagg cggattccga agcggcattg cgccagagtt gcttgtgggg
      241 attggccggg gttatcggtg ccgagcagcc tcagctttgg ggcggcctga ttgacatcgc
      301 ggtcggtgac gatatcgccg attgcgcagc ggcgttgtct gcggtgctac cgacgccagc
      361 caagtccgtg ttatcgctgc gcgacggcga attctttgaa acggcattgg cgccgatctc
      421 cggcgaaccc gtgcgagagc cggtgcggtg ccgtcccgat gccgcgtacc tgatcaccgg
      481 cggcatgggc gcactcgggc tattgatggc gggctggctc gccgaccgcg gtgcgcgtcg
      541 actaatactg gcgggccgca caccgctgcc tccgaggcgc gagtgggacg gtgacattac
      601 cgacacggat gtgcacgaca agatcgatgc gatcagggca ctcgagatgc gtggcgtctc
      661 tgtcgatacc gtggccctcg acattgggtc gcgcgaggcg gtgcaagctc tgctcgcccg
      721 acgagacagc gatggggcgc cgccgatacg cggtgtcata cacgccgcgg gagtgaccga
      781 aggccaacta cttactgagg tctcggacag ccggcttcac cgcaccatgt ggccgaaaat
      841 agctggggct cagtcgttgc acgaggcttt cccgccggcc agcctcgact tcttcttcct
      901 gacagcctcg gccggtacgg tatttggtat tccggggcag ggcgcgtatg cggccgcaaa
      961 ttcatacctc gattgcctgg cccgagcgcg tcaccgacaa gggtgcaaca ccgtcagcct
     1021 ggactgggtg gcctggcagg gactcgggtt cgggtccgac gcgcaaatag tcatccaaga
     1081 gcttgagcgc gtggggtccc ggccggtcac cccgtacgaa gccttcaccg cgtgggagca
     1141 cgtgacgtgt tacgacgtcg cccaagctgt catggccccg atgccatccg ctgaggagac
     1201 tgcgatcggc atctccaatg cccaccggcc gtcggccccg gtgcgggcct ggtcgcagat
     1261 gacctccgag gatctgcgcg ctgaactcca aaacgggctc cggacgatcc tcgcaaccga
     1321 acttcgaatc cccatagacg aggtcgagtt ggaccgcccc ttcgcggaga tggggctgaa
     1381 ctcggtgatg gcgatgtcga ttcggcgaga ggtagagcag ctagtcggga tcgaactgtc
     1441 ggcgacgatg ctctggaacc acccgacaat cgcgtccttc tcgggttatc tcgccgaaca
     1501 gcttgtgcca caggaaagct cggacggcga cagtgaaatc gacggttcga cggacagcgt
     1561 gttggactcg ctgttcgaca gcgtcgagtc ggagagcagg atctgatgcc ggcggaaagg
     1621 atgttctgat gtcaccattt gagtcttcta tcccagccat gctcgctgat cgcgcgcaga
     1681 agcagccgga tgacatcgcc tacaccttca ttgactacga agtagacctt gctgggtacg
     1741 ccgaaagctt gacctgggct gaagtccacc acagggcaag ggtcgtcgcc cagaagcttg
     1801 caacgtgcgg gtcgcccggc gaccgggcgg tgatactggc cccgcagagc ctggagtaca
     1861 tcatcgcgtt cctgggcgcg atacaagccg gtttcgtggc tgtgccactg tcggtgccgc
     1921 agttcggcca acacgacgaa cgagtgtcag gggcgctcgg cgactccacg cccgccgcga
     1981 tacttacgac atctgccgtg gtcgacgacg tcaggaaata cgcccaagcg cagcccaggc
     2041 aacgggctcc ccgagtggtc gaaatcgacg ccctggactt cgactcgccg atttccgatg
     2101 cagctctcgt cccgatctca aagccggcat atctgcagta cacctctggc tcgacgcgtc
     2161 agccggccgg cgtcatggtg acgcaccaga acatcctgac gaacattgaa caactgcagc
     2221 ccgactactt cgagcggagc ggaggtattt caccgcagga tatgacgatc gtgtcgtggc
     2281 ttccgttgta ccacgacatg ggactccttg tcgggttgtt cctgggaatg gcgtttggac
     2341 acccggcggt gttgatgagc cccgtcgcgt tcatgcagaa gccggccagg tggatgcagc
     2401 agctggcgat gaacagcagc acgttcacgg ccgcgccgaa cttcgcctac gacttggcgg
     2461 tgcgccggac atcggatgac gatctggccg gcatggatct gggcggcgtg caggtcatga
     2521 tc
//
LOCUS       DASRJA010000365         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1557051,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000365 DASRJA010000000
VERSION     DASRJA010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557051"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFC01_16210"
     CDS             <1..509
                     /locus_tag="VFC01_16210"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZN81210.1"
                     /translation="LFADCRSAADVQECVRIVRAETPEDKGTYGVATRRFTYMGYGGT
                     KEYVQALRDIVVAIMIEKPGTVDTLEDVLAVKGVDMVQWGGSDYSMGMGRPGERYSPE
                     LRVVERRVFETAIRMGVPPRAEIATADQAKYFLDMGVRHFSVGTDITILHQWWKSQGE
                     DMRKALGH"
     gene            543..1577
                     /locus_tag="VFC01_16215"
     CDS             543..1577
                     /locus_tag="VFC01_16215"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HZN81211.1"
                     /translation="MARDMSLAAAQTEPSGHSRIPVLDVGAYLAGVAGSAAPLARAIA
                     RTCEDTGFLVVANHGVPQHLVDDTFAVAQQFFARPEPDKLALKVGRYNIGYLPFGGQV
                     VRHSPVNKNTKPNFSESFYISRDRAPDHPDIVNNKPLVGLNRWPEDMPELRAAATAYY
                     RAMEAMTTRLVPLFALALDLPADYFAAAFAEPSCTIRLIHYPPHPSPEDNEFGFAPHT
                     DNNFITFLAQSKLPGLEVRTAEGDWIRPPAVPDTFVVNTGAMLARYSNDRFRATPHRV
                     INRNDRSRYACPFFLGPNHDVVVEPVPSCVGPDNPPKYEPTTYGAFTQHLLTLNFTHR
                     QAGGSGEYAG"
     gene            complement(1647..1976)
                     /locus_tag="VFC01_16220"
     CDS             complement(1647..1976)
                     /locus_tag="VFC01_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009475732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN81212.1"
                     /translation="MPNQPVPRPTPSWAADFPKLREATDNWLFGDIWERSGLSKRDRS
                     LITVAMLTALYRHEQLPGHVNRALDNGVTKEELVEVITHATFYSGWPTGVQGAVIAKK
                     VFESRGL"
     gene            complement(2037..>2429)
                     /locus_tag="VFC01_16225"
     CDS             complement(2037..>2429)
                     /locus_tag="VFC01_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81213.1"
                     /translation="SEAEEGWEYFHSQLLAAQHHYLEWNNPGYAGIPGYEEYLKRQTA
                     DVGVADAGLAARRATQPIGTPVEIIEKIRALQHAISLEMVVIHFFYGGMPRAKAEKSL
                     RLFAEKVLPAVQAMPTPINPASLGQPRS"
BASE COUNT          402 a          822 c          788 g          417 t
ORIGIN      
        1 tcctgtttgc cgactgtcgc tccgctgccg acgtgcagga gtgcgtgcgc atcgtgcgcg
       61 cggaaacccc ggaggacaaa ggaacctacg gcgtggccac ccgtcggttc acctacatgg
      121 ggtatggcgg cacgaaggag tacgtccagg ccctgcgcga catcgtcgtg gccatcatga
      181 tcgagaagcc ggggacggtc gacaccctcg aggacgtcct ggccgtcaaa ggggtcgaca
      241 tggtgcagtg gggcggctcc gactactcga tgggcatggg acggcctggc gagcgctact
      301 cgcccgagct acgcgtggtg gagcgccgcg tgttcgaaac ggccatcagg atgggtgtgc
      361 cgccccgtgc cgagatcgcc accgcggatc aggcgaagta cttcctcgac atgggcgtcc
      421 ggcatttctc cgtcggcacc gacatcacca tcctgcacca gtggtggaag tcccagggtg
      481 aggacatgcg caaggcgctc ggccactgaa cccacgacat cctggtgaag gttcgccata
      541 acatggcgcg tgacatgagt ctcgctgccg cccagaccga gccgtcgggc cacagccgta
      601 tcccggtgct cgatgtcggc gcgtatctcg ccggcgtggc ggggtcagcg gcgccgctgg
      661 cgcgtgccat cgcccgcacc tgtgaagaca ccgggtttct cgtcgtcgcc aatcacggcg
      721 tgccgcagca tctggtcgac gacaccttcg ccgtcgccca gcaattcttc gcgcgtcccg
      781 aaccggacaa gctcgcgctc aaggtcggca ggtacaacat cggctacctg ccgttcggcg
      841 ggcaggtcgt gcgccattcg ccggtcaaca agaacaccaa gccgaatttc agcgagagct
      901 tctacatcag ccgcgaccgc gcccccgacc accccgacat cgtcaacaac aagccgctgg
      961 tcgggctgaa ccgctggccc gaggacatgc ccgagctgcg cgccgcggcg accgcgtatt
     1021 accgggcgat ggaggcgatg acgacacggc tggttccgct attcgcgctg gcactcgacc
     1081 tgcccgccga ctatttcgcc gcggccttcg ccgagccgag ctgcacgatt cgcctgatcc
     1141 actacccgcc gcacccgtcg cctgaggaca acgagttcgg cttcgcaccg cataccgaca
     1201 acaacttcat cacgtttctg gcgcagtcga agctcccggg cctggaagtg cgcaccgctg
     1261 agggcgactg gatcaggccg ccggcggtgc cggacacgtt cgtcgtcaat accggcgcga
     1321 tgctggcgcg ctactcgaac gatcgcttcc gggcgacgcc tcaccgcgtc atcaatcgca
     1381 acgatcgctc gcgctacgcc tgcccgttct tcctcgggcc gaaccacgat gtggtcgtcg
     1441 aaccggtccc aagctgcgtc ggccccgaca acccgcccaa atacgagccg accacctacg
     1501 gcgccttcac tcagcacttg ctgacgctca acttcaccca ccgccaggct ggcggcagtg
     1561 gcgagtatgc ggggtaagct gaacgacgct caggcgggcg cgggcagcgc ggtgcttcgc
     1621 tggcgtcgca ggagctgcgc ggacgtctag aggccgcgag actcgaagac cttcttggcg
     1681 atgaccgcgc cctgcacgcc ggtgggccag ccgctgtaga acgtcgcgtg cgtgatcacc
     1741 tcgaccagct cttccttggt gacgccgttg tccagcgcac ggttgacgtg gccgggcagc
     1801 tgctcgtgcc ggtagagcgc ggtcagcatg gcgacggtga tcaaactgcg gtcacgcttg
     1861 gagaggcccg agcgctccca gatgtcgccg aacaaccagt tgtcagtggc ctcgcggagc
     1921 ttggggaaat cagcggccca gctgggtgtg ggtcggggta cgggctgatt gggcatcgag
     1981 tgctcctccg tgtcgtgttt gcaagtgtcc cgtcgggcga ttgtacgcgc gctccctcag
     2041 ctcctgggct ggcccagtga cgccggattg atcggcgtcg gcattgcctg caccgcggga
     2101 aggactttct ccgcgaacag ccgcaggctc ttctctgcct tcgcgcgtgg catcccgcca
     2161 tagaaaaagt ggatgaccac catctccagg ctgatggcgt gctgcagcgc cctgatcttc
     2221 tcgatgatct cgacgggcgt cccgatcggc tgggtggcac ggcgcgccgc gaggcccgcg
     2281 tcggccacac cgacgtcggc ggtctggcgc ttgagatact cctcgtagcc gggaatgccg
     2341 gcgtagcccg ggttgttcca ctcgaggtag tgatgctgag cggcgagaag ctggctgtgg
     2401 aagtactccc agccttcctc ggcctcgga
//
LOCUS       DASRJA010000366         8537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_578461,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000366 DASRJA010000000
VERSION     DASRJA010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8537
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578461"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFC01_16230"
     CDS             <1..633
                     /locus_tag="VFC01_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN81214.1"
                     /translation="CVAVGPIADELVAKIAERTNTIKTGDGRGDADMGPLVTKVHRDK
                     VASYIDAGEADGAKIVVDGRGVTADGGADGFWLGPTLIDHVTPEMSVYTDEIFGPVLS
                     VLRVQTYDDALDLVNSNPYGNGTAIFTNDGGAARRFQNEVEVGMVGINVPIPVPMAYY
                     SFGGWKASLFGDTHAHGTDGVQFFTRGKAITSRWLDPSHGGIELGFPQNK"
     gene            656..2038
                     /locus_tag="VFC01_16235"
     CDS             656..2038
                     /locus_tag="VFC01_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZN81215.1"
                     /translation="MTTTDIAQEVSTDLGAKAARHLWGHFAKHGEGITPPIITRGEGV
                     TIWDDKGKSYIDGLSGLFVVQVGHGRKELAEAAAKQAETLAFFPLWSYATPPAIELSE
                     RVAGYAPGDLNRVFFTTGGGEAVESAWKLAKNYFKLTGKPGKHKVVSRAIAYHGTPQG
                     ALAITGLPAFKQQFEPLTPGGFRAPNTNFYRAPEPYAYDIKAFGQYCANRIAEAIEFE
                     GPDTVAAVFLEPVQNAGGSIPPPPGYFERVREICDEYDVLLVSDEVICAYGRIGSMFA
                     CDDFGYVPDIITCAKGLTSGYSPIGAMVASDRLFEPFNDGKTTFAHGYTFGGHPVSSA
                     VALANLDIFEREGLNDHVKETAPAFRATLEKLVSLPIVGDVRGEGFFYGIELVKDQAT
                     KETFTNDECTRLLPQLTTALLEAGLYCRADNRGDPVVQVAPPLISGQKEFDAIYEILH
                     GVLDEAGRRL"
     gene            2678..3580
                     /locus_tag="VFC01_16240"
     CDS             2678..3580
                     /locus_tag="VFC01_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HZN81216.1"
                     /translation="MTPVIGARPGERRGSSGEEVRSHYDLSNEFFRLWQDPTQTYSCA
                     YFERDDMTLQEAQIAKIDLALGKLGLQPGMTLLDIGCGWGSTLMRAIEKYDVNVIGLT
                     LSHNQKRHIEPLFAESDSPRSKEVRLQPWEEYDEAVDRIVSIGAFEHFGFEKYDDYFK
                     KTFNLMPDDGVMLLHTIVVPSAEEVAERQLRTTMSLMRFIRFILTEIFPGGRLPQVAQ
                     IEEHATKAGFNVTRVQALRPHYVRTLSTWAANLEAHKDEAIAITSEEVYERYMKYLTG
                     CAALFRDGYTDVCQFTCEKAYSAR"
     gene            3660..4544
                     /locus_tag="VFC01_16245"
     CDS             3660..4544
                     /locus_tag="VFC01_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane mycolic acid synthase family
                     methyltransferase"
                     /protein_id="HZN81217.1"
                     /translation="MTDYRVSSTKMRPHFEESQAHYDMSDDFFGLFQDPSRTYSCAFF
                     EREDMTLEEAQMAKIDLALGKLGLQPGMTLLDIGCGWGSVMKRAMEQYDVNVVGLTLS
                     KNQCAFCEQLLDQVDTDRSRQVVLRGWEEFDEPVDRIVSIEAFEAFGKPRYAAFFETA
                     YRIQPGDGRMVLETIFTHPPSYWPTKGIAITMSDLRFMRFIAQEIFPGGQVPSEEDVV
                     EFSRGAGFSLEETRLLNPHYVRTLDTWAANLEAGRDDAIASTSNETYERYMRYLTGCA
                     DFFRRGITEVGQFTLAKG"
     gene            5349..5576
                     /locus_tag="VFC01_16250"
     CDS             5349..5576
                     /locus_tag="VFC01_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81218.1"
                     /translation="MPSRSVKISRPNNARRRSDFPGTSAFVVAAASSALGTHFTGTVP
                     AAARSQLPPNRVGVLGGHLYTIGPRNVGDDV"
     gene            5670..6728
                     /locus_tag="VFC01_16255"
     CDS             5670..6728
                     /locus_tag="VFC01_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020111240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN81219.1"
                     /translation="MYSKDDVSYFIVDAHIALWDGRPENQRNIHGKQFIDCFYDYHRN
                     LSPESEVWSYEEYLYYGAERLMNDLFRDGYIDYAIFQPVNLGEFYHRGFSQTEESFAL
                     ATANPDKLTYNHYFDSRNGEAGLDQLRADAERFNLRGVKLYTAEWHGDSRGWKLDDPW
                     AYRYFEVCQELGIRNIHVHKGPTIRPLDRDSFDVADIDHVATDFTDLNFIVEHCGLPR
                     LDDFCWIATQEPNVHAGLAVAMPFIHTRPKYFAQIIGELLYWIGEDRIQFASDYAIWT
                     PRWLVEAFVDFQIPEELGDYPAITTEQKKKILGLNAAKMYDIPVPPELQLPAAAEPAV
                     GPRGADKTAESADHLVGA"
     gene            6730..7461
                     /locus_tag="VFC01_16260"
     CDS             6730..7461
                     /locus_tag="VFC01_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HZN81220.1"
                     /translation="MNRHDQAYAALGTVVDPELDEPITELGFVRSLVITGDDVEVHLR
                     LPTSFCAPNFAYLMASDAKDVLVKLDWTEHVVVELDDHHDSDVINTGLAADAGYRGTF
                     GQEAEDSLDELRVTFRRKAHTAAMERCLTELLRVDPQRDVASLGRVQLRELPDNQTTA
                     ALLRRRAALGLSVAPTELVMVDHEGHGYASDDVSLALRRARSTRISIDGNAHFCRGLL
                     RTRYPEADHAQTPAFVPLSALTVTH"
     gene            7497..8522
                     /locus_tag="VFC01_16265"
     CDS             7497..8522
                     /locus_tag="VFC01_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZN81221.1"
                     /translation="MRAVQVTGYHQNMQLAEVPIPTPTGPFDVVVKIGGAGVCRTDLH
                     ILEGQWAEKSQVQLPYTIGHENAGWVHAIGSAVTNVREGDKVIVHPLITCGLCRACRS
                     GDDVHCEASSFPGIDTNGGYADYLKTSARSVVKIDEALEPADVAALADAGLTAYHAAA
                     KAARRLTPRDRCVIIGAGGLGHIGIQVLKALSPAELIVVDRNPEAVKLAVSIGAHHGV
                     VADGTQVEHVLELTGGNGAEAVIDFVGEGGSTGEGVRMLHRAGDYHVVGYGENINVPT
                     IDIISTEINFIGNLVGSYNDLCDLMALAARGAVTLHTAKYRLDDFQSAIDDLDAGNVR
                     GRAILTP"
BASE COUNT         1728 a         2812 c         2551 g         1446 t
ORIGIN      
        1 tgcgtggcgg tcggcccgat cgccgacgag ctggtcgcca agatcgccga acgcaccaac
       61 accatcaaga ccggcgacgg ccgcggcgac gccgacatgg gtccgctggt caccaaggtg
      121 caccgcgaca aggtggcctc ctacatcgac gccggcgaag ccgacggcgc caagatcgtc
      181 gtcgacggcc gcggagtgac cgccgacggc ggagccgacg ggttctggtt gggcccgacg
      241 ctgatcgacc atgtgacccc cgagatgagc gtctacaccg acgaaatctt cggacccgtg
      301 ctctcggtgc tgcgcgtcca gacctacgac gacgccctgg atctggtgaa ttcgaaccca
      361 tacggcaacg gcaccgcgat cttcaccaac gacggcggcg ccgcccgccg cttccaaaac
      421 gaggtcgaag tcggcatggt cggcatcaac gtccccatcc cggtgccgat ggcctactac
      481 agcttcggcg gctggaaagc ctcactgttc ggcgatacac atgcccacgg caccgacggg
      541 gtccagttct tcacccgcgg caaagccatc accagccgct ggctcgaccc cagccacggc
      601 ggcatcgaac tcggcttccc ccaaaacaaa tagatcaaga caggattaga catacgtgac
      661 aactaccgat atcgcacaag aggtttcgac ggatctgggc gcaaaggccg cccgtcatct
      721 gtggggtcac ttcgcgaagc acggtgaagg catcacgccg ccgatcatca cccgcggcga
      781 aggcgtgacg atctgggacg acaagggcaa gagctatatc gatggcctgt caggcctttt
      841 cgtcgttcag gtcggccacg gccgcaagga actcgccgag gccgcagcca aacaggccga
      901 gacgctggcg ttcttcccgc tgtggtccta cgccacaccg cccgcgatcg aactgtccga
      961 gcgcgtcgcg ggctatgcac caggtgatct gaaccgggtg ttcttcacca ccggcggcgg
     1021 cgaggccgtc gagtcggcgt ggaagctggc caaaaactac ttcaagctga ccggcaaacc
     1081 cggtaagcac aaggtggttt cgcgcgcgat cgcctatcac ggcaccccgc agggtgcgct
     1141 ggcgatcacc ggcctgccgg ccttcaaaca acaattcgag ccactgacac cgggtggctt
     1201 ccgcgcaccc aacaccaatt tctaccgcgc acccgagcct tatgcctacg acatcaaggc
     1261 attcgggcag tactgcgcta accggatcgc cgaggccatc gagttcgagg gccccgacac
     1321 cgtcgcggcc gtcttcctcg agccggtgca gaacgccggc gggtccattc cgccgccacc
     1381 cggatacttc gagcgcgtcc gcgagatttg cgacgagtac gacgtgctgc tggtctccga
     1441 cgaagtgatc tgcgcctacg gccgcatcgg ctcgatgttc gcctgtgacg acttcggcta
     1501 tgtgcccgac atcatcacct gcgcaaaggg tctcacatcc ggctactcgc cgatcggtgc
     1561 gatggtcgcg agcgaccggc tcttcgagcc gttcaacgac ggcaagacga ccttcgccca
     1621 cggctacacc ttcggcgggc atccggtgtc gtcggcggtg gcgttggcca acctcgacat
     1681 cttcgagcgc gaaggcctca acgaccacgt caaggaaacc gcacccgcgt tccgcgccac
     1741 gctggagaag ctcgtgagcc tgccgatcgt cggcgacgtc cgcggcgaag gcttcttcta
     1801 cggcatcgaa ctcgtcaagg accaggccac caaggaaacc ttcaccaacg acgagtgcac
     1861 tcgcctactc ccccagctca ccaccgcact cctcgaggcc gggctgtatt gccgtgccga
     1921 caaccgcggc gacccagtcg tccaagtggc gccgccactg atcagcgggc agaaggaatt
     1981 cgacgccatc tacgaaatcc tgcacggcgt actcgacgaa gcgggtcgcc gactctagat
     2041 caattccgcg aaactgtatt ccagcagcga aagttcgagt aagcacctgc tggaatacag
     2101 tttcggcgaa aatggcccct gcactagcgt cgcgacgcag cagcctgctg aggtgcgggg
     2161 tccaagacgc gcgcagcgct tgatcgctca ggaaacacca tgttgcactc acctcgtcca
     2221 gccagtcctc gaacgcccgc cgcgccagca gtgccgccgt acgcggtccc cgcgcaccga
     2281 ggctgaccct cccgtcgagc acctgctcgg cgtacgtcag cagttcagcc cggtcggtca
     2341 tgaccccagc ttcgccaggt cgccgaccgc cagccgtgtc gacttcaccg cgtcgcgata
     2401 gtccgtcaca ccgctgtgtg cacctttggc ggcggcggac gatcacgccg gcaagccgcg
     2461 gcaccggcaa gctggagcat ccgaacctcg tcgcagtgca caacgtgttc gacgttgacg
     2521 accgctcaac gtttcggtca attaaccgtg accggctccc tgggcgttag tagtctcgtg
     2581 gtatcgcgtt ggaagaaacc cacccgatcg atcacgtacg cgattgctcg tgtcaagagg
     2641 gcgaacagtg acagatacac cgagcgaaac gcagaccata acgcccgtta ttggggcccg
     2701 gccgggcgag cggcgggggt cgtcgggcga ggaagtccga tcgcactacg acctgtcgaa
     2761 cgagttcttc cggctgtggc aagaccccac ccagacctac agctgcgcat acttcgagcg
     2821 cgacgacatg acgctgcagg aggcgcagat cgccaaaatc gacctcgcgc tgggaaagct
     2881 cggcctccag cccggaatga cattgctcga tatcggctgc ggctggggct ccacgctgat
     2941 gcgagcgatc gagaaatacg acgtcaacgt gatcggcctg acgctttctc acaaccagaa
     3001 aaggcacatc gaaccactgt ttgccgagtc ggacagcccg cgcagcaaag aagttcggct
     3061 gcagccgtgg gaggagtacg acgaggcggt cgaccgaatc gtgtcgatcg gcgcattcga
     3121 gcacttcggc ttcgagaagt acgacgacta cttcaaaaag accttcaact tgatgcccga
     3181 cgacggcgtg atgctgctgc acaccatcgt agtaccgagc gccgaagagg tggcggaacg
     3241 ccagctgcgg acgacgatgt cgctgatgcg cttcatccga ttcatcctca ccgaaatctt
     3301 ccccggcggt cgactcccgc aggtcgcgca aatcgaagag catgccacca aggccggctt
     3361 caacgtgacg cgggtccagg cgctgcgccc gcactacgtc cgcaccctga gcacgtgggc
     3421 ggcaaatttg gaggcccaca aggacgaggc gatcgcgatc acctccgaag aggtctacga
     3481 gcggtacatg aagtatttga ccggctgcgc tgccctgttc cgcgatggct acaccgacgt
     3541 gtgccaattc acctgcgaga aggcctactc agccaggtga ggtgaattcg ttattcgacc
     3601 aaagggaaac gagaacgtag cgatcgcaat ccgatccacc cgggggaggt caatcgcata
     3661 tgactgacta cagagtgagc tcgacaaaga tgcggccaca cttcgaggag agtcaagccc
     3721 attacgacat gtccgatgat ttcttcgggc tgtttcagga tccgtcgcga acctacagtt
     3781 gcgcgttctt cgaacgcgag gacatgacac tcgaagaggc gcagatggcc aagatcgacc
     3841 ttgcgcttgg caagctgggt ttgcagccgg ggatgacgct gcttgacatc ggttgcggct
     3901 ggggttcggt gatgaagcgc gcgatggagc agtacgacgt caacgtcgtc ggcctgactc
     3961 tgagcaagaa tcagtgcgcc ttctgcgagc agcttctgga ccaggtcgat accgaccgct
     4021 cccgccaggt ggtcttgcgt ggctgggagg agttcgacga gcccgtcgac cgcatcgtca
     4081 gcatcgaggc gttcgaggcg ttcggaaaac cgcggtatgc ggcattcttc gagacggcct
     4141 accgtattca acccggcgat ggccggatgg tactggagac catatttacc catccgccga
     4201 gctattggcc cacaaagggt atcgcgatca ccatgagtga cctgagattt atgcggttca
     4261 ttgcccaaga gatatttccg ggcggccaag tgccatccga ggaagacgtc gtcgaattct
     4321 cgcgcggcgc tgggttttcg ctggaagaaa ctcggctctt gaacccgcac tatgtccgga
     4381 cgctagatac ctgggcggcg aacttggaag ccggccgcga cgacgcgatc gcctcgacat
     4441 caaatgaaac ctacgagcgc tacatgcgtt acctcacggg atgcgctgac ttcttccggc
     4501 gcggcatcac cgaggtggga cagttcaccc tggcgaaggg ataaggcgcg ggtcgggcag
     4561 acggcccacc atgtgctccg ccaccaccac gaggtacgcc cgcgccggga tggatcgttc
     4621 atcagcccgt acagtaacgc tgggctctac gccgcgaaac aacgccactg ggttccacgc
     4681 gcgaatcatc ttgcgtacct tagcattagc ccctttgtga ccgttttgtt cgagcccgcc
     4741 caccgccgag cgtgcagcaa aacgggtatt cccctgccgc cccagggtgg cgatggtcgg
     4801 caagttcgca acgacaacac gttgagcagc aacgccttta gcggcagaac aacgccgccg
     4861 gtcaacaaga actcatcgac agcgggatcg gtggcctgtg acagcgccga ggccaaggaa
     4921 caaccaacgg gctcggcctg ttcggccgct cactcgtcgg cttggatcgc gaggatgctg
     4981 tcgaagcgtt cggcaccacc tggacgaatc gaagttcacg gccgaccaga tccggttcat
     5041 caacctgatc atcaacgaac tcgccttggg gacgcacttg cccgacgccg ccaccccatg
     5101 tgtgcggtta tcacgagcca aaggtccacc ggccacctgg ggagtccacc cgaggctctc
     5161 ccaagcgcct gcgcaaatcg tgaaacgatc tggccgctgg cggtcagcaa catgacggga
     5221 agtgcccact ccgacattgc gaagaccgac acgcactcgc tccccaccgc gtcgagaacc
     5281 gcgacgagcc cgtcggtcca ggcctgcatc gcgggacggt catggatcag caccggtagg
     5341 agctcccggt gccgagcagg tcggtcaaga tcagccggcc gaacaatgcc aggcgcagaa
     5401 gtgacttccc tggcacgagc gcattcgtcg tcgccgcggc cagcagtgcg ctcggcaccc
     5461 atttcaccgg caccgtgcca gccgccgcca gatcgcagct gccgcccaac cgggtcggtg
     5521 tgctcggcgg ccacttgtac accatcggcc cccggaacgt cggcgatgac gtctgaaatt
     5581 cggccgtcag ctggcaccgg ctcgggccga gccgccataa gcttttccca gacggtgtga
     5641 caaccgtcac acgaagggag agtcctgaaa tgtacagcaa ggacgacgtc agctacttca
     5701 tcgtcgacgc ccatattgct ctgtgggacg gccggccgga gaaccagcgc aacattcatg
     5761 gcaagcagtt catcgactgc ttctacgact accaccgcaa cctgtcgccg gaatcagagg
     5821 tgtggtccta cgaggagtac ctctactacg gcgctgagcg gctgatgaac gacctgttcc
     5881 gggacggcta catcgactat gcgatcttcc agccggtgaa tctcggcgag ttctaccatc
     5941 gcgggttcag ccagaccgag gagtcgttcg ccctggcaac ggccaatccc gacaagctga
     6001 cctacaacca ctatttcgac tcacgcaacg gcgaggcagg cttggaccag ctgcgcgccg
     6061 acgccgagcg attcaatctg cggggcgtga agctctacac cgctgaatgg cacggggatt
     6121 cgcgcggctg gaagctcgac gacccgtggg cataccggta cttcgaagtc tgccaagagc
     6181 tcggtatccg caacatccac gtgcacaaag gcccgacgat caggccgctc gaccgcgatt
     6241 ccttcgatgt tgccgacatc gatcatgtcg ccacggactt caccgacctg aacttcatcg
     6301 tcgagcattg cggcctgccc cgccttgacg acttctgctg gatcgcaacg caagagccca
     6361 acgtgcatgc cggccttgcg gttgcgatgc cgttcatcca cacccggccg aaatacttcg
     6421 cgcaaatcat cggtgagctt ctttactgga tcggcgagga ccgcatccaa ttcgcatccg
     6481 actacgcaat ctggacgccg cgctggctgg ttgaggcctt cgtcgacttc cagattcccg
     6541 aggagctagg ggactacccg gcgatcacca ccgagcagaa gaagaagatt ctgggcctga
     6601 acgcggccaa gatgtacgac atcccggtgc cgccggagct gcagctgccc gcggcggcag
     6661 aacccgccgt cggtccgcgg ggcgccgaca aaaccgccga gtctgccgac cacctggtcg
     6721 gggcctgacg tgaatcggca tgatcaggcc tatgcggccc tgggcaccgt ggtcgatcct
     6781 gagctcgacg aaccgatcac cgaactgggg ttcgtccgct cgctggtgat caccggcgat
     6841 gacgtcgagg ttcacctgcg gttgcccaca tccttttgtg cgccaaactt tgcatacctc
     6901 atggcgtctg atgccaaaga cgttctggtg aaactggatt ggacagagca cgtggtggtt
     6961 gaactcgatg accaccacga ttcggacgtc atcaataccg gcctggccgc tgacgccggt
     7021 tatcggggaa cctttggcca ggaggccgaa gacagtctgg acgagctgcg ggtgaccttc
     7081 cggcgcaagg cgcataccgc agcgatggag cgctgcctaa cggagttgct ccgggtcgac
     7141 ccccaacgcg atgtggcatc gctggggcgg gtgcagctgc gcgagctgcc ggacaaccag
     7201 accacggcgg cgttgctgcg tcgccgggcc gcgctcggat tatccgttgc gccaaccgaa
     7261 ctcgtgatgg tggaccacga gggccacggc tacgcctccg acgatgtttc ccttgcgctg
     7321 cgccgcgccc gatccacgcg gatctccatt gacggcaacg cccacttctg ccgcggactg
     7381 ctgcgcaccc gctatcccga ggctgaccat gcccagacgc cggctttcgt acctctgtcc
     7441 gcactcaccg tgacgcattg aaccaggatc cgaaaggaac actaccgatg agcaccatgc
     7501 gagccgtaca agtaaccggc taccaccaga acatgcagct ggccgaggta ccgatcccga
     7561 caccgactgg gccgttcgat gtggtggtaa agatcggcgg tgcgggtgta tgccgaaccg
     7621 atcttcacat cctcgagggc cagtgggccg agaagtctca ggtgcagttg ccgtacacaa
     7681 tcgggcacga gaatgccggc tgggttcatg caatcgggtc cgcggtgacc aacgtccgcg
     7741 agggggacaa ggtcatcgtc cacccgttga tcacctgcgg gctgtgccgg gcctgccgct
     7801 ccggcgacga cgtgcattgt gaggccagtt cgtttcctgg catcgacacc aacggcgggt
     7861 acgccgacta cctgaagaca tcggcccgca gcgtcgtgaa aatcgatgaa gccctggaac
     7921 cagcggatgt cgccgcgctt gccgatgccg gcctcaccgc ctaccacgcg gcagccaagg
     7981 ccgcgcggcg tctcacgccc cgtgaccgct gcgtcatcat cggtgccggt ggacttggcc
     8041 acataggcat tcaggtgctg aaggcgctga gtcccgccga gctcatcgtc gtcgaccgca
     8101 acccggaggc cgtcaagctc gcggtgtcca ttggtgccca ccacggagtc gttgccgacg
     8161 gcacccaagt tgaacacgtt cttgagctca ccggtggcaa cggtgcggag gctgtcatcg
     8221 acttcgtcgg cgagggcggc tccaccggcg aaggggtgcg gatgcttcac agggccggcg
     8281 actatcacgt cgtgggctat ggcgaaaaca tcaacgtccc cacgatcgac atcatctcca
     8341 ccgagatcaa cttcatcggc aatcttgtcg gctcgtacaa cgacctttgc gacctgatgg
     8401 cgctggccgc ccgcggcgcc gtcacgctgc acaccgcgaa ataccgactc gacgacttcc
     8461 aatcagcgat cgacgacctc gacgccggga acgtacgcgg ccgcgccatc ctcaccccat
     8521 agagcggcaa caagatg
//
LOCUS       DASRJA010000367         5857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2830888,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000367 DASRJA010000000
VERSION     DASRJA010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2830888"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..224
                     /locus_tag="VFC01_16270"
     CDS             66..224
                     /locus_tag="VFC01_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81222.1"
                     /translation="MLFPIMIFLMVLSPLYIPIAVTVVHEFGNWRGNRVARPVGSLRQ
                     FRAQPVAA"
     gene            244..372
                     /locus_tag="VFC01_16275"
     CDS             244..372
                     /locus_tag="VFC01_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81223.1"
                     /translation="MTPTGVTFVAFTTLRAPGSLGTTSAAASVVVTVQDTPSAPRC"
     gene            571..1464
                     /locus_tag="VFC01_16280"
     CDS             571..1464
                     /locus_tag="VFC01_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN81224.1"
                     /translation="MELRELTAFVAVAEEGGMSAASRRLHISQPALSQTVSSLERQLG
                     VKLLERSSTGVRPTEAGAALLSEARAILARHDQALRRMSEFTTEGGGVLRLGVPIELP
                     ADLLERTLAKFSANISGTRVIPRHLSTAAQLSALRSGELDVGLVRERPAAEDFDAMLV
                     LRENLGVLLASEVAAGLVGPDGVQLEALCGLQWLGFPRSNSPAWHDELTAILRTHGID
                     LGPPPPEDQLLIAAVKLAAVSAGRSFALAPENWPHPIPESVTWSALIGHPLVRRTWAV
                     WPANSRRRDIGQLVTAFEAPA"
     gene            complement(1465..2205)
                     /locus_tag="VFC01_16285"
     CDS             complement(1465..2205)
                     /locus_tag="VFC01_16285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN81225.1"
                     /translation="MAGTLEGSTALVTGATAGIGRAIALQLATLGAEVVVHGRSAERG
                     AETVQEIEKAGGKARFVAADLSDADDVRHLAAESGPVDILINNAGVYKFGATAETDDA
                     FFDEHVNINLRAPYILVQKLVPAMAERGKGVVVNLSTIAAGVPARQGGIYGATKAGIE
                     LLTRVWADEFGRSGVRVNAVQAGPTETPGTAVTPGLTDGLGQLTTLGRAAAADEIANA
                     VTFLASPAASYVNGAILQANGGQLAIAP"
     gene            2404..3219
                     /locus_tag="VFC01_16290"
     CDS             2404..3219
                     /locus_tag="VFC01_16290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN81226.1"
                     /translation="MTNSVRIGVQLWPGGTPDYRTWREAVIKAEELGADAIFGYDHFH
                     KPFTVPSEDGLPELLPEQPDVNNFEGWTALASWAEITHRAEIGLLVTGIGYRNPDLLA
                     DMARTVDHISGGRVILGLGSGWYEKDYTVYGYDYGTVRSRMDLFEERLARVERRLTQL
                     VPPPVRNIPILVGGMGVKRTLPIVAKYADIWHTFASPGEYRRKNALLKDLVAQAGRDE
                     KQIERAVHWTGRENADAFLQEGVTLFTTEIHPTADGYDFSELKDMIDWRDQVH"
     gene            complement(3646..5139)
                     /locus_tag="VFC01_16295"
     CDS             complement(3646..5139)
                     /locus_tag="VFC01_16295"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZN81227.1"
                     /translation="MTNTITHWINNKPYPGTSGATAAVTNPARGAVTGEVALASVADA
                     RAVIDAAAAAFPAWRDTSLAKRSTILFKFRELLNARKPELAKIITAEHGKVVSDALGE
                     VSRGQEVVEFACGVPHLLKGGFTENASTKVDVYSVRQPLGPVGIISPFNFPAMVPMWF
                     FPIAIAGGNTVVLKPSEKDPSASLWIAELWAEAGLPPGVFNVLQGDKTAVDELLTNPA
                     IKAVSFVGSTPIARYVYETGTAHDKRVQALGGAKNHAVILPDADLDLAADAMVNAGFG
                     SAGERCMAISACVAVGPIADELVAKIAERAATIKTGDGTGDADMGPLVTRAHRDKVAA
                     YVDAGAAAGATVVVDGRTVTPDGGPDGFWLGPTLLDHVTPAMSVYTDEIFGPVLSVLR
                     VDTYDEALELINTNPYGNGTAIFTNDGGAARRFQNEVEVGMVGINVPIPVPMAYYSFG
                     GWKSSLFGDSHAHGTEGVHFFTRGKAITSRWLDPSHGGLNLGFPQNK"
     gene            complement(5224..>5857)
                     /locus_tag="VFC01_16300"
     CDS             complement(5224..>5857)
                     /locus_tag="VFC01_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN81228.1"
                     /translation="IAGYHPQTDGELLVDGEATHFNSPKDALAKGIATVYQNLAVVPL
                     MPVWRNFFLGQEIRKKTFPFPLDSNAMRATTLTELSKMGIDLPDVDVPIGSLSGGQRQ
                     CVAIARAVFFGARVLILDEPTAALGVKQSGVVLKYITAAKEAGFGVVFITHNPHHAHM
                     VGDHFVLLNRGQQKLDCTYDEIDLEHLTQQMAGGDELEALSHELRGGKRT"
BASE COUNT         1005 a         1855 c         1900 g         1097 t
ORIGIN      
        1 ctcagcgatg agttccccaa gacaaaaggc caacgattcc gcccgcggag aatgcgagag
       61 atccaatgct cttcccgatc atgatcttcc tgatggtgct gtccccgctg tacatcccga
      121 tcgcggtcac ggtggttcac gagttcggca attggcgcgg caatcgtgtc gctcgtccgg
      181 tcggctcgct tcgccagttc cgcgcgcagc cggtggccgc gtaatacgca tacactcacg
      241 aacgtgacgc caaccggcgt cacgttcgtc gcgttcacga ccctgagggc accgggatcg
      301 ctgggcacga catcggcagc ggcgtccgtg gtggtcacgg ttcaggacac gccgtctgcg
      361 ccgaggtgct gacgcggaca tcaacggtgg tgtagacgcg aattgcaaag cggtacttga
      421 gcttcacaag acggccaaga gtcgtggacc gtcgaaagac ttaaccctgg accacgaccc
      481 gaggccgatg aggttaaacc atggatgggg cggttagttt gcgctccgca tcggcttatc
      541 ggacgaggat cccggccata agctgaagcg atggagttgc gggagctgac cgccttcgtc
      601 gccgtcgccg aggagggtgg catgtcggcg gcgtcgcgtc ggcttcacat cagccagccc
      661 gcgttgtcgc aaacggtcag ctcccttgag cgccagctcg gcgtgaagct gctggaacgc
      721 agtagcaccg gcgtgcgacc aaccgaagcc ggcgccgcgc tgttgagcga ggcgcgtgcc
      781 attctggcca gacatgacca ggccttgcgg cggatgtccg agttcaccac cgaaggcggc
      841 ggcgtgctgc gcctcggggt gcccatcgag ctgcccgctg atctgctcga gcgcaccctt
      901 gccaaatttt ccgcgaatat ttcgggcacc cgggtgatac cgcgccattt gtccacggcc
      961 gcacagcttt ccgcgttacg aagcggtgaa ctcgacgtgg ggctggtgag ggagcgaccc
     1021 gccgccgagg acttcgacgc gatgctggtg ctgcgcgaga acctcggggt gctgctggcc
     1081 tccgaggtcg ccgctggact ggttggcccc gacggtgttc agctcgaggc gctgtgcggc
     1141 ctgcagtggc ttggtttccc gcgctccaac agccctgctt ggcacgacga gttgaccgcc
     1201 attctgcgca cgcacggcat cgacctcggc cctccgccgc ccgaggacca gttgctcatc
     1261 gccgcagtca agctggcggc tgtcagcgcg ggtcgctcgt tcgcattggc gccggagaat
     1321 tggccgcacc ccatcccgga gtccgtgacg tggagcgcac tgatcggcca cccgctagtg
     1381 cgacgcacat gggccgtgtg gccggccaac tcgcgtcgcc gcgacatcgg ccagctggtc
     1441 accgccttcg aggcaccggc gtaattacgg cgctatcgcg agctgaccgc cgttggcctg
     1501 caggatcgcc ccgtttacgt agctggccgc gggggaggcc aaaaacgtga cagcattggc
     1561 gatttcgtca gcggctgctg cccggccgag cgtagtgagc tgacccagac catcggtgag
     1621 accgggtgtg acggcggtcc caggagtttc cgttggaccg gcctgcacgg cgttgactcg
     1681 taccccggag cggccgaatt cgtcggccca gacacgggtc aacagttcga tgccggcctt
     1741 ggtagcgcca tagattccgc cctgccgtgc cggcactccg gcggcgatgg tgctcaggtt
     1801 cacaacgacg cccttgcccc gctcggccat ggccggcacc agcttctgca ccagtatgta
     1861 cggcgcgcgc aggttgatgt tgacatgctc gtcgaagaac gcatcgtcgg tctcggccgt
     1921 ggcgccgaac ttgtatacgc cggcattgtt gatcaggatg tcgaccgggc cggactcggc
     1981 ggccaggtgc cgcacgtcgt cggcatcgct gaggtcggct gcgacgaaac gcgctttccc
     2041 tccggccttt tcgatttctt gcaccgtttc ggcgccgcgc tcggcgctac ggccatggac
     2101 cacaacctcg gcaccgaggg tggccagctg tagcgcaatc gcgcggccga tgcccgcggt
     2161 tgctcccgtt acaagtgcgg tcgacccttc caacgtgcca gccatgagaa tctcctcgca
     2221 gaaatcgtcg aacgttgact cccagctaag cacgcaaacg catggcatcg attccagttc
     2281 gcacggcgtt ctgcttatgg cggcgattgg tggcagcgat aggcgtgcgg gattggccac
     2341 atcctgtgag atgttctccg gttcgacagc gcacgaggcg ctgcgggagg caggaggaca
     2401 atcgtgacca attccgttcg gattggtgtt cagctgtggc cgggtggcac ccccgactac
     2461 cgaacgtggc gagaggccgt gatcaaggcc gaggagctag gcgccgacgc gatcttcggt
     2521 tacgaccatt tccacaaacc gttcaccgtg ccgtccgaag acggccttcc cgagctgctg
     2581 cctgaacagc ccgatgtcaa caacttcgag ggctggacgg cgctcgcgtc gtgggccgag
     2641 atcacccatc gagccgagat cggtctgctg gtcaccggca tcggttatcg caacccggat
     2701 ctgctggccg atatggcccg cacggtcgat cacatcagcg gtgggcgagt aatcctggga
     2761 ctgggctccg gctggtacga aaaggattac accgtctacg gatacgacta cggcaccgtt
     2821 aggtctcgga tggacttgtt cgaggagcgc cttgcccgcg tcgagcgacg gctcacccag
     2881 ttggttccac cgccagtgcg caatattccg atcctggtcg gcggtatggg ggtgaagagg
     2941 acgctgccaa tcgtcgccaa gtacgcggac atctggcaca ccttcgccag cccgggcgaa
     3001 tatcgacgca agaacgcgtt gctgaaagat ctcgttgcgc aggccgggcg cgatgagaag
     3061 caaattgagc gggctgtcca ttggaccgga cgcgaaaacg ccgatgcgtt ccttcaggag
     3121 ggcgtcaccc tcttcaccac cgagatccac cccacggccg acgggtacga cttctcggaa
     3181 ctgaaggaca tgatcgactg gcgcgaccaa gtccattaac agttagtgaa gcgcgccgct
     3241 cctgtcacct ggctcccgca tcgcgggcgc cgacttagag cgacgcggcg actggttgct
     3301 tttgcttggc cggtgtgtcc gatcagctct accccgtcag tcgcattcac ctcatctcgc
     3361 ccgcgctgac catcgccgga caccctgcgg gaactgccgc ggatatcggg gacgaccatt
     3421 ctgcgtcaaa gtgatgggtc agtcacggac agcgacccgt ggtcacagtg aatggcttct
     3481 tgcattgatt tgggctgtcg gaggttctgg cggatccaag acatctattg accaaatcca
     3541 gtagcggcgt tgggctcgaa gcgctgcgtt gtctgtgtcc gctcaccgat gagtattcgg
     3601 aggtcgactg ccgacgcgaa cattcgccgg gcgacacttc ggtgtctatt tgttttgggg
     3661 gaagccgaga ttgaggccgc cgtggctggg gtcgagccag cggctggtta ttgctttgcc
     3721 gcgggtgaag aagtgcacgc cttcggtgcc gtgggcgtgg ctgtcgccga acagcgagct
     3781 tttccatccc ccgaagctgt agtaggccat cggcacgggg atgggcacgt tgatgccgac
     3841 catgccgact tcgacttcgt tttggaagcg ccgcgcggcg ccgccatcgt tggtgaagat
     3901 ggcagtgccg ttgccgtagg ggttggtgtt gatcagctcc agcgcctcgt cgtaggtgtc
     3961 tacacgcagc accgagagca ccgggccgaa gatctcgtcg gtgtagacgc tcattgccgg
     4021 ggtgacgtga tcgaggaggg tgggcccgag ccagaacccg tccggcccgc cgtcgggcgt
     4081 cacggtgcgc ccgtcgacga cgacggtggc gccggcggcc gcgccggcat cgacatatgc
     4141 ggccaccttg tcgcggtgtg cgcgggtcac caacgggccc atgtcggcgt cgccggtgcc
     4201 gtcgccggtc ttgatcgtgg cggcacgttc ggcgatcttg gcaaccagct cgtcggcgat
     4261 cgggccgacc gccacgcagg ccgagatggc catgcagcgc tcgccggccg acccgaaccc
     4321 ggcgttgacc atggcgtcgg cggccaggtc cagatcggcg tcgggcagga tcaccgcgtg
     4381 atttttcgcc ccgcccaacg cttggacgcg tttgtcgtgc gcggtgccgg tttcatagac
     4441 atagcgggcg atcggcgtgg acccgacaaa cgacaccgcc ttgatggcgg ggttggtcag
     4501 cagttcgtcg acggcggtct tatcgccctg cagcacattg aaaaccccgg gcggcaggcc
     4561 ggcctcggcc cacagctcgg cgatccacag cgacgccgag ggatccttct ccgagggttt
     4621 gagcaccacg gtgttgcccc cggcgatggc gatcgggaaa aaccacatcg gcaccatcgc
     4681 cgggaaatta aacggcgaaa tgatgccgac cgggcccagc ggctggcgca ccgaatacac
     4741 atccaccttg gtggaggcgt tctcggtgaa gccacccttc aacaagtgcg gcaccccgca
     4801 ggcgaactcg acgacctcct ggccccggga cacctcaccg agcgcatcgg agaccacctt
     4861 gccgtgctcg gcggtgatga tcttggccaa ctccggctta cgggcattca gtagctcgcg
     4921 gaacttgaac aggatcgtgc tgcgcttggc cagcgaggta tcccgccacg ctggaaacgc
     4981 cgcggccgcc gcatcgatca ccgcgcgcgc atccgccaca cttgccagcg ccacctcacc
     5041 ggtcaccgca cccctcgccg gattggtcac cgccgcggta gcaccgctgg tgcctgggta
     5101 gggcttgttg ttgatccaat gcgtgatcgt gttggtcatc gttgttccta attccgatat
     5161 ttggtgacga agtcggtcat ggccttctgt tgattgcgag agacctcgac gaagctggcg
     5221 tacttaggtg cgcttgccgc cgcgcagctc gtgtgagagt gcttcgagtt cgtcgccgcc
     5281 tgccatctgc tgggtcaaat gttccaggtc gatctcgtcg taggtgcagt cgagcttctg
     5341 ctgaccgcgg ttgagcagca cgaagtgatc gccgaccatg tgcgcgtgat ggggattgtg
     5401 cgtgatgaac accacgccga agccggcttc cttggccgcg gtgatgtact tcagcaccac
     5461 cccggactgc ttgacgccca gcgcggccgt gggctcgtcg agaatcagca cccgcgcacc
     5521 gaagaacacc gcgcgcgcga tagcgacgca ttgacgctga ccacccgata gagagccgat
     5581 cggcacgtcg acgtcgggaa ggtcgatgcc catcttcgac agctcggtca gtgtggtggc
     5641 ccgcatcgcg ttggaatcca acgggaacgg gaaagtcttc ttacggatct cctggcccag
     5701 gaagaagttg cgccataccg gcatcaacgg cacgacagcg aggttctgat acaccgtcgc
     5761 aatgcccttg gccaatgcgt cttttggcga attgaagtgc gtggcttcgc cgtcgaccag
     5821 aagttcgccg tcggtctgcg ggtgatatcc ggcgatg
//
LOCUS       DASRJA010000368         5815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_666124,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000368 DASRJA010000000
VERSION     DASRJA010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666124"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFC01_16305"
     CDS             <1..468
                     /locus_tag="VFC01_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN81229.1"
                     /translation="LPFLRAQRSGHIIQVSSIGGIVAFQNLGAYHASKWALEGFSQAL
                     AQEVAPFGVHVTLIEPGGFGTDWAGASSKRSTPLPDYKEVHEEADRLRAQRSDQPGDP
                     QASARAVLKIVDAPEPPLRVFFGELPLALAKADYENRLKTWEEWQPVSVEAQG"
     gene            complement(543..1670)
                     /locus_tag="VFC01_16310"
     CDS             complement(543..1670)
                     /locus_tag="VFC01_16310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZN81230.1"
                     /translation="MRVAIVAESFLPNVNGVTNSVLRVIEHLRRTGHEALIIAPDTPR
                     GEPPADKIHDGIRVHRVPSRMFPKITSLPLGVPRPRMVKVLRGFDPDVVHLASPALLG
                     YGGLHAARFLGVPTVAVFQTDVAGFAESYGIGFTARAAWAWTRHLHSRADRTLAPSTA
                     AMENLTAHRIPRVHKWARGVDITGFAPSARDEMLRHNWSPEGKPIVGFVGRLAPEKHV
                     ERLAALNGRDDLQVVVVGDGVDRAKLETVLPSAVFTGALYGDELAAAYASMDVFVHPG
                     EHETFCQAVQEAMASGLPVVAPNAGGPRDLVAPYRTGLLLDVDEFDTKLAESVDHLLA
                     ERQRYSVAARRSVLGRTWPAICDELLGHYEDVIGQRSRKAA"
     gene            1804..2478
                     /locus_tag="VFC01_16315"
     CDS             1804..2478
                     /locus_tag="VFC01_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZN81231.1"
                     /translation="MRISRVMAIIATALMVAAVGCTKQVAGAAESDPNKPPLSVSKDG
                     YGIVAGFDNAPAKIEIYTEPQCNHCADLQHDFGDQLAYYIAVGQLEVTYRPMTFLDDK
                     SGGYSGQVSNALFLAAEKPAATGTQFQRFVEQLWAHQRPGGTPPSKDELHEYATKAGM
                     SDSVAKRISDGGSAVNTKEMDDANFEFLYEIDSMDLGTPTAYDLNKGEKLDIYDNDWL
                     NKLMQS"
     gene            complement(2465..2962)
                     /locus_tag="VFC01_16320"
     CDS             complement(2465..2962)
                     /locus_tag="VFC01_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HZN81232.1"
                     /translation="MKRLKTLWRWLIPRLARDPDETRSQRIFRRIRIGIVIAACLVTL
                     QSVLLVFGAWRNDRQIERHMGVAEANVLSAGPRRSTIEFVTPDRVTYRPELGVLYPSE
                     LDTGMRIYVEYDKNNPDLVRVQDRNASLAIIPAGSIAVVGWLIAAAALTGVTLIERRA
                     DLKTA"
     gene            complement(2959..4584)
                     /gene="menD"
                     /locus_tag="VFC01_16325"
     CDS             complement(2959..4584)
                     /gene="menD"
                     /locus_tag="VFC01_16325"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HZN81233.1"
                     /translation="MNPSTAQARVVVDELIRGGVRDVVLCPGSRNAPLAFALQDADRA
                     GRIRLHVRIDERTAGYLAIGLAIAERAPVCVAMTSGTAVANLGPAVVEANYARVPLIV
                     LSANRPYELLGTGANQTMEQFGYFGTQLRANISLGLAEDAPERMDSLNAQWRSATCRV
                     LVAATGSRTANAGPVQFDIPLREPLVPDADESGIYAPVGRPDGRPWTYTPPVTFDQPL
                     DIDLTPDTVVVAGHGAGVHPNLAMLPTVAEPTAPPAENPLHPFALRLVHPKQVIMLGR
                     PTLHRPVSALLADPSVPVYALTTGPRWPDVSGNSQATGTRAVTTGEPSPNWLKRCDDV
                     NRHAIEAVRTQLKAHPLKTGLHVAAAVADAIKPGDQLVLGASNPVRDAALVGLNTHDI
                     KVRSNRGVAGIDGTVSTAIGAALAHDGRTIALIGDLTFVHDSSGLLIGPTEPTPRNLT
                     IVVSNDNGGGIFELLEQGDPRFSDVSSRIFGTPHDVDVGALCRAYHVESRQIEADGLV
                     DALDEPFEGMRVLEVKADRSSLRALHASIKAAL"
     gene            complement(4587..5369)
                     /locus_tag="VFC01_16330"
     CDS             complement(4587..5369)
                     /locus_tag="VFC01_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN81234.1"
                     /translation="MNLAYDDRGTGDPVLFIAGRGGAGRTWHLHQVPEFLRAGYRCIT
                     FDNRGIGATETADGFTTDTMVADTAALIEKLDAAPVHIVAVSMGAFIAQELMVARPEL
                     VRSAVLMATRGRHDRTRDFFRGAELELIKSGIDLPPSYEAKVRLLESFSPMTLNDDVA
                     VRDWIDMFTMWPTKPTAGIKTQLTIAPEGNRLSAYRSITAPVLVIGFQDDVVLPAHLG
                     REVADAIPNGSYLEIPHTGHLGFIEKPQVVNAAALKFIADSL"
     gene            complement(5438..>5815)
                     /locus_tag="VFC01_16335"
     CDS             complement(5438..>5815)
                     /locus_tag="VFC01_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZN81235.1"
                     /translation="HWLVLPMLKPFGVEAVGDERIVHEGKFVTAAGVSAGIDLAMWLF
                     GQICGEAKAKAVQLAIEYDPQPPFDSGHVSKASAATKASATAQLSKETFKQHQVSQSV
                     RLLWDAAIQRARGGKQGRTRVKV"
BASE COUNT          992 a         1940 c         1942 g          941 t
ORIGIN      
        1 ctgccgttcc tgcgggcaca gcgcagcggc cacatcatcc aggtgtcgtc gatcggcggc
       61 atcgtcgcgt tccagaacct gggcgcctac cacgcgtcga agtgggcgct cgagggcttc
      121 tctcaggcgc tggcccaaga agtcgcgccg ttcggcgttc acgtgacgct gatcgagccc
      181 ggcgggttcg gcaccgactg ggctggggcg tcgtctaagc gctctacccc tctgcccgac
      241 tacaaagagg tgcacgagga ggcagaccga ctgcgcgccc agcgcagcga ccagcctggt
      301 gatccgcagg cgtcggcccg cgcagtgctc aagatcgtcg acgcgcccga gccgccgctg
      361 cgcgtgttct tcggggagct gccactggcg ctggccaagg ccgactacga gaaccggctc
      421 aagacatggg aagagtggca gccggtctct gttgaagcgc agggctaggc ggcgcgaaca
      481 gacgcaaaag tgcccatttt ccgacgattt tgtgcaggtt tgcagctgct cgccagaaga
      541 aatcaggcgg ccttgcggct gcgctggccg atgacgtcct cgtagtggcc gagcagctcg
      601 tcgcagatcg caggccacgt gcggccgagc acgctgcgac gtgcggccac cgaatagcgc
      661 tggcgctcag cgagcaggtg gtcgacagac tcggccagct tggtgtcgaa ctcatcgacg
      721 tccagtagca gcccggttcg gtagggggcg accaagtcgc gcggccctcc cgcgttcggg
      781 gcgacgaccg gcagacccga cgccatcgcc tcttgcacgg cctggcagaa cgtctcgtgc
      841 tcgccggggt ggacgaagac gtccatgctg gcgtatgccg cggcgagctc atcgccgtac
      901 agcgcaccgg tgaaaacggc cgacggcagg acggtttcga gcttcgctcg gtcgacgccg
      961 tcgccgacga ccaccacctg aaggtcgtcg cggccattga gcgccgccag ccgctcgacg
     1021 tgcttctccg gcgccagccg gcccacgaac ccgacgatcg gcttgccctc cggggaccag
     1081 ttgtgccgca gcatttcgtc gcgcgccgac ggcgcgaagc cggtgatgtc gacgccgcgc
     1141 gcccacttgt gcacacgcgg aatgcggtgc gctgtcaggt tttccatcgc tgcggtcgac
     1201 ggggccagcg tgcggtcggc gcggctgtgc aagtgccgcg tccacgccca tgcggcgcgg
     1261 gccgtaaagc cgatgccgta gctctccgcg aaacccgcta cgtcggtttg gaatacggcg
     1321 acggtcggca cgccgaggaa tcgcgcagca tgcagcccgc cgtagccgag caatgccggt
     1381 gacgccaaat gcacgacgtc cgggtcgaaa ccgcgcaaca ctttgaccat ccgcggccgt
     1441 ggcacaccga gcggcagcga ggtgatcttg ggaaacatcc gcgacggcac ccgatgcacg
     1501 cggattccgt cgtgaatctt gtcggcgggc ggctcgccac gcggggtgtc cggggcgatg
     1561 atgagtgcct catggccggt gcggcggaga tgctcgatca cccgaagcac cgagttggtg
     1621 acgccgttga cattcgggag gaaggactct gcgacgattg caacgcgcac accacgactg
     1681 tcccatcaac ggctgtccca aaggttgcag cagggcatac gacacgcaaa gacctcgtgc
     1741 cgatgatcgg ccggtcggca gatcggtatt cgagtatcgt gcgcacaaag aagggcggtt
     1801 gacatgcgga tttcgcgggt catggcgatt atcgcgacag cgctgatggt cgcggccgtc
     1861 ggctgcacca aacaggtcgc gggcgccgcg gaatccgatc ccaacaagcc gccgctatcg
     1921 gtgagcaagg acggttacgg catcgtcgcc ggcttcgata acgcgcccgc gaagatcgag
     1981 atctacaccg agccgcaatg caaccactgt gcggacctgc aacacgattt cggcgaccag
     2041 ctcgcctact acatcgccgt cgggcagctc gaggtcactt accgcccaat gacgttcctc
     2101 gatgacaaga gcggcggcta ttcgggacag gtgagcaatg cgctgttcct cgcggccgag
     2161 aagccggcag cgaccggcac acagtttcaa cggttcgtcg agcagttgtg ggctcaccag
     2221 cgtccggggg gtacgccgcc gtcgaaagat gaactgcacg aatacgcgac gaaggccggc
     2281 atgtccgaca gtgtcgccaa gcggatcagc gatggcggat cagcggtgaa caccaaagag
     2341 atggatgacg cgaacttcga gttcctctac gagatcgact cgatggactt aggcacccca
     2401 acggcttacg acctcaacaa gggcgagaag ctcgacatct acgacaacga ctggctcaac
     2461 aagctcatgc agtcttgagg tctgcgcgcc tctcgatcaa cgtgacaccc gtcagcgcgg
     2521 cggccgcgat cagccaaccc accaccgcga tcgagccggc cgggatgatc gccagcgatg
     2581 cgttacggtc ttgcacccga accagatccg ggttgttctt gtcgtactcg acgtagatcc
     2641 gcataccggt gtcgagctcc gacggataca gcacgcccag ttccgggcgg taggtcacgc
     2701 gatcgggtgt gacgaactcg atcgtcgagc gccgcgggcc cgcactgagc acattcgctt
     2761 ccgccacacc catatggcgt tcgatctgcc ggtcgttgcg ccacgccccg aacaccaaca
     2821 gcaccgactg caaggtcacc aagcaggcgg cgatcacgat gccgatgcgg atccgccgga
     2881 agatccgttg cgaccgggtt tcgtccggat cgcgcgccag acgcggaatc agccagcgcc
     2941 acaacgtttt caggcgcttc atagggcagc tttgatcgat gcgtgcagcg cccgcagcga
     3001 cgaccggtcg gccttcacct ccagcacccg catcccctcg aacggttcat caagggcgtc
     3061 gacgaggccg tcggcctcga tctgcctgct ttcgacgtgg taggcccggc acagcgcgcc
     3121 gacgtcgacg tcgtgcggcg tcccgaagat gcgcgacgac acgtcggaga accgggggtc
     3181 gccctgctcg agcagttcga agatgccgcc gccgttgtcg tttgacacca cgatcgtcag
     3241 gttgcgcggc gtcggctccg tcggcccgat cagcaggccc gagctgtcat gcacgaacgt
     3301 caagtcaccg atcaacgcga tcgtgcgtcc gtcgtgtgcc agcgccgccc cgatcgccgt
     3361 cgacactgtg ccgtcgatgc cggccacgcc gcggttcgac cgcaccttga tgtcgtgtgt
     3421 gttcagcccg accagcgccg cgtcgcgcac cgggttcgac gcgcccagta ccaactgatc
     3481 ccccggcttg atggcgtcgg ccaccgcggc cgcgacatgc agcccggttt tcagcgggtg
     3541 cgccttgagt tgcgtgcgaa cggcctcgat cgcgtgtcgg ttcacgtcgt cgcagcgctt
     3601 gagccagttg gggctgggct cgccggtggt gaccgcacgc gtgcccgtgg cctgcgaatt
     3661 ccccgacacg tcgggccagc gtggccctgt cgtcagtgcg tacaccggca ccgacgggtc
     3721 ggccagcagc gccgacaccg gccggtgcag cgtggggcgg ccgagcatga tcacctgctt
     3781 cgggtggacc agccgcagtg cgaacgggtg cagcgggttt tcggcgggcg gcgcggtcgg
     3841 ctcggcgacc gtcggcaaca tggccagatt ggggtggacg ccagcgccgt ggccggcgac
     3901 gacgacggta tccggcgtga ggtcgatgtc cagcggctgg tcgaacgtga cgggcggcgt
     3961 gtatgtccac ggcctgccgt ccggccggcc cacaggcgcg tagatgccgg actcgtcggc
     4021 gtcgggcacc agcggctcgc gcagcggaat gtcgaactgc accggaccgg cgttggcggt
     4081 gcgagatccc gtggcggcca ccagaactcg gcaggtcgcc gagcgccact gcgcgttgag
     4141 cgagtccatt cgctcggggg cgtcctcggc cagaccgagg ctgatgttgg cgcgcagctg
     4201 ggtgccgaag tagccgaact gttccatcgt ctggttggcg ccggtgccca gcagttcata
     4261 gggccggttg gcgctcagca cgatcagcgg cacccgcgcg tagtttgcct ccacgaccgc
     4321 gggcccgagg ttggccaccg cagtgccaga cgtcatcgcc acacagactg gcgcccgctc
     4381 ggcgatggcg agcccgatcg ccagataacc cgcggtgcgc tcgtcgatgc ggacgtgcaa
     4441 ccggatgcgg cctgcccggt cggcgtcttg cagcgcaaag gctagcggcg cgttacgcga
     4501 ccccgggcac agcaccacgt cgcgcacgcc gccacgaatc agttcgtcga cgaccacgcg
     4561 ggcctgcgca gtcgatgggt tcaccactac agactatcgg cgatgaattt cagggccgcg
     4621 gcgttgacga cctgcggctt ttcaatgaag ccgaggtgcc cggtgtgggg aatctccagg
     4681 tagcttccgt tcgggatggc gtcggcgacc tcgcgaccca ggtgcgcggg cagcacgacg
     4741 tcgtcttgaa agccaatgac cagaaccggt gcggtaatac ttcggtaagc actcagccgg
     4801 ttgccctccg gcgcgatggt gagctgcgtc ttgatgccgg ccgtcggctt ggtgggccac
     4861 atggtgaaca tgtcgatcca gtcgcgcacg gcgacgtcgt cgttcagcgt catcggcgaa
     4921 aagctttcca gcaggcgaac tttcgcctcg tacgacggcg gcaggtcgat gccggacttg
     4981 atgagctcga gttcggcgcc gcggaagaag tcgcgggtgc ggtcgtggcg gcctcgggtg
     5041 gccatcagca cagccgacct gacgagctcc ggcctggcca ccatcagctc ctgcgcgatg
     5101 aacgcgccca tggacaccgc gacgatgtgt accggcgcgg cgtcgagctt ctcgatcagc
     5161 gccgccgtgt cagcgaccat ggtgtcggtc gtgaagccgt cggcggtctc cgtcgcgccg
     5221 atgccgcggt tgtcgaaggt gatgcagcga tagccggccc gcagaaactc tgggacctgg
     5281 tgcaggtgcc aggtacgccc ggcgcccccg cgaccggcga tgaacagcac cgggtcgcct
     5341 gtaccgcggt cgtcgtaggc cagattcacg ccgacgacgc tacttgaggc gatttgtgca
     5401 cggaaatccg cgctcagcgc gggaaatcgt gcacaaatca cactttgacg cgagtgcggc
     5461 cctgcttgcc gccccgcgcc cgctggatcg cggcgtccca cagcagccgc accgactgac
     5521 tcacctggtg ctgcttgaag gtctccttgc tcagctgcgc cgtggccgac gccttggtgg
     5581 cagccgacgc cttcgacacg tgacccgagt cgaacggcgg ctgcgggtcg tactcgatgg
     5641 ccagctgcac cgccttcgcc ttggcctcgc cgcagatctg gccgaacagc cacatcgcga
     5701 ggtcgatgcc ggcggacaca cctgcggcgg tcacgaactt tccctcgtgc acgatccgct
     5761 cgtcaccgac cgcctcgacg ccgaacggtt tcagcatggg cagtacgagc cagtg
//
LOCUS       DASRJA010000369        19829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_572969,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000369 DASRJA010000000
VERSION     DASRJA010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 19829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19829
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572969"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFC01_16340"
     CDS             complement(<1..654)
                     /locus_tag="VFC01_16340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN81236.1"
                     /translation="MNRFEGKVAVITGAGSGIGRALAVNLATKGAKLALSDIDTEGLA
                     GTVRQAESLGATVKSDRLNVAEREAVLAYADAVVTHFGEVHQVYNNAGIAYNGDLEKS
                     EFKDIERIMDVDFWGVVNGSKAFLPHVIVSGDGHVVNISSLFGLIAVPGQNAYNAAKF
                     AVRGFTEALRQEMLVAKHPVKVTCVHPGGIKTAVARNATVADGQNAQSFAEFFDKHMA
                     "
     gene            complement(651..1574)
                     /locus_tag="VFC01_16345"
     CDS             complement(651..1574)
                     /locus_tag="VFC01_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN81237.1"
                     /translation="MNINKRVIRAGLRASRPLFASHRVSVNGKRAMVNMLTGAARPPK
                     GTTFDRVTIADVAVERVQPPLGGTNGTLIYLHGGGYALGSAKGYRGLVAHLAAASGMT
                     AIIPDYSRAPEARYPVALEEMVAVYSRLLEDGLDPKSTVVAGDSAGGGLTLALAMALR
                     DRGIAAPAALGLICPWANLAIDIDQLRPKLRDPLILPTMTAEWAPQYAGTADPMSPGI
                     SPVYGDMTGLPPIVMQTAGDDPIRCDALDIARAVAVAGTPTVDHRVFENLWHVFHLQV
                     SVLAEAREAVADLGAKLHNHVHTNNVKVAVK"
     gene            complement(1626..3104)
                     /locus_tag="VFC01_16350"
     CDS             complement(1626..3104)
                     /locus_tag="VFC01_16350"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN81238.1"
                     /translation="MNAQHFDILIIGAGLSGIGTACQVSAEHPNKTIAVLERRERLGG
                     TWDLFRYPGIRSDSDMYSFGYKFRPWQELKVLADGASIRQYIADTAAEYGIDEKIHYG
                     LKIVTADWSTPDSRWTVTALHEASGETRTYTAGFLISCTGYYNYDAGYLPTFPGADQF
                     KGRFIHPQHWPEDLDYTGKKVVVIGSGATAVTLVPTMAGDAEHVTMLQRSPSYIFSVP
                     ALDKISQVLGRFLPDKWVYKFARKRNIAIQRYLYLACKRWPAQMRRFLLWQVRRHVGP
                     SVDMSHFTPKYMPWDERLCAVPNGDLFKVLASGEASIVTDHIETFTEKGILLKSGREL
                     EADTIVAATGLDIQMLGGMELSVDGEARKLNDQMTYKGVLVENIPNLAWVFGYTNAPW
                     TLKSDIAGLYLCRLFKHMDDNGHTVATPLDIDNSAMDVGMLDQLQSGYVQRAKDTLPR
                     QGSKYPWKVLMHYGKDSKMLLQDPVEDNQLQFDKTTVDAVAV"
     gene            3232..4260
                     /locus_tag="VFC01_16355"
     CDS             3232..4260
                     /locus_tag="VFC01_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZN81239.1"
                     /translation="MVSLVRATNLWGYGDLVRELGADPQPFLSRFNIQPGVEYEEDAF
                     IPFEAAARLLEVSADELNCPDFGLRLSRWQGMDILGPIAVIARNAHTVLDGLESIASY
                     LYIHSPALKLTVASPTAGADVRFTFEVVEPGLSYVLQAYELSLANGARMIRLMGGPEA
                     RPRAISFIHEQRGSDAAYSEALGCRVRFGQKWCGFDLPEHLAGKRIVGADPETRRIAT
                     KYLESNYLPSTATLSERVAELARRLLPTGQCSVEAIGDQLALHPRTLQRSLAAEGVRC
                     QDLIERERRDQAAKYLAEPRLHLSQIATLLGYTEQSTFNRSCRRWFGKTPRQYRADLS
                     VSPSRVHR"
     gene            complement(4504..5970)
                     /locus_tag="VFC01_16360"
     CDS             complement(4504..5970)
                     /locus_tag="VFC01_16360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZN81240.1"
                     /translation="MRRLTSLDAFFLATEDERTVANVSALVILERKRANGRRLTRHAV
                     KTLFAQRLHLLPPLRWQLAEVPLGVAHPSWVDGPVDLDFHVREFALAAPGDDAVLAEQ
                     VARLAAEPTNRSRPLWEAYLIHGLSGDRVALLIKLHHAAVDGVSGGEILGALLDESPN
                     GREVAPTTSRQTESVPNQLGMLVRGSADLPRQQLAAVGAVRPALAHLDKVATIRSLPG
                     IGEIAGLVGRFAPGSPAESAASPAIVAPRTAFNARITPNRSLVMVSLPLPEVLQLKEA
                     HGATVNDIVVSLCAGALRNWLDGKDELPDERLLAMVPVSVRTPAERSTFGNKVSAMVV
                     PLPTDEADPAGRIRACRDSLQQAKQQQQAEPPNLMQHANDLVPPVLFGPAMRTVLRLA
                     SSRRLNPAANVIISNVPGPRTPRYCAGARVLANYPVSTIVDGMALNITLFSYLDTLHI
                     GVMADPELVPDVDLLAQALVNELELQVSLLKSAKSKRR"
     gene            complement(6181..6441)
                     /locus_tag="VFC01_16365"
     CDS             complement(6181..6441)
                     /locus_tag="VFC01_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HZN81241.1"
                     /translation="MRNSGVGTLVCARDPDRWTTVADEGAKALCRECPRRWRCAQEAC
                     ETPGAEGIWAGVLIPAAGRGRRFALKQLRSLAERNGFPVRKR"
     gene            6550..7167
                     /locus_tag="VFC01_16370"
     CDS             6550..7167
                     /locus_tag="VFC01_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN81242.1"
                     /translation="MMVVLPQTDAVSADERSSIERIRSAALRSFATYGASATSLRTVA
                     EAAGVSVGLVQHHFTNKAGLIKAVDDHVMAVVVELIAQPLPAPPADSIAEVGSRVTRF
                     MSGYPDVVDYVGRALIDGSPLGTTIFDTLAAFGMARWSQRKERGEVRPDVDLTWAALN
                     SLVLALGAMILRGHIERHLPEPFTTPAQLQRWQDAVNKLLRGGAV"
     gene            complement(7177..8013)
                     /locus_tag="VFC01_16375"
     CDS             complement(7177..8013)
                     /locus_tag="VFC01_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN81243.1"
                     /translation="MTVSALSDRVLAESANRRPVIELRRGGRALGGSYLYEGDELITG
                     WHSHEVHQIEYAIGGVVEVETASAHYLLPPQQAAWIPAGLEHQATMNSDVRTVAVMFD
                     RELVADGGDRARILAVSPLIREMMIYALRWPIDRPDGDAVSDTFFRTLANLVSEALDH
                     EAPLSLPTSENPIVAAAMAYTKEHLDSVTASEVGRAVSVSERTLRRLFQDTLGLPWRT
                     YLLHARMLRAMALLAAPGQSVQETSTAVGFDSLSAFTRAFTQFCGETPSTYRKRVGGA
                     RV"
     gene            8039..9301
                     /locus_tag="VFC01_16380"
     CDS             8039..9301
                     /locus_tag="VFC01_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN81244.1"
                     /translation="MKTDDLILMSIDDHVVEPPDMFLNHVPAKYKDEAPIVVTDDKGV
                     DQWMYQGRPQGVSGLNAVVSWPAEEWGRDPAGFAEMRPGVYDVHERVRDMNRNGILAS
                     MCFPTFTGFSARHLNMHREHVTLVMVSAYNDWHIDEWAGSYPDRFIPIAVLPTWNPEA
                     MCAEIRRVAAKGCRAVTMPELPHLEGLPSYHDDEYWGPVFQTLSEENVVMCLHIGTGF
                     GAISMAPNAPIDNLIILATQVSAMCAQDLLWGPAMRNYPDLKFAFSEGGIGWIPFYLD
                     RSDRHYTNQKWLRRDFGDKLPSDVFREHSLACYVTDKTSLKLRHEIGIDNIAWECDYP
                     HSDCFWPDAPEQVLAELTAAGADDSDINKITWGNSCRFFSWDPFARTPKNQANVGALR
                     AKGADVDVSIRPRREWARLYAEKQLAKA"
     gene            9362..9769
                     /locus_tag="VFC01_16385"
     CDS             9362..9769
                     /locus_tag="VFC01_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012690654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN81245.1"
                     /translation="MGLIRVREAAELLGVSDDTVRRWIDDGSLRASHDESGRKVIPGD
                     VLAKFARANPAAAPKDPLTVASSARNRFAGLVTKVVTDKVMAQVEMQCGPFTVVSLMS
                     TDAVRELGLEPGSVAVAVVKATTVIVETPGGQS"
     gene            9802..10518
                     /gene="modA"
                     /locus_tag="VFC01_16390"
     CDS             9802..10518
                     /gene="modA"
                     /locus_tag="VFC01_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZN81246.1"
                     /translation="MIGGCGSPEKPAAENQKIMVFAAASLKKTFTDLGEQFKTENPGA
                     SVEFSFAGSSDLVTQLTQGAQADVFASADTTNMDKATQAGVLAGDPVNFASNTLTIAV
                     APGNPKKIASFKDLTQQGLNVVVCAPQVPCGSATQKVEQATGVTLNPVSEESSVSDVL
                     NKVTTGQADAGLVYVTDAKGAGDKVAAVAFPEAAGAVNTYPIAVLKSSKNSELARKFV
                     DLVTGESGQKVLDAAGFAKP"
     gene            complement(10515..12890)
                     /locus_tag="VFC01_16395"
     CDS             complement(10515..12890)
                     /locus_tag="VFC01_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HZN81247.1"
                     /translation="MRVDGRDITVSGSLLQPLTRRTNDILRVVLAAVFLAAVITSSLI
                     TRNEWVALEKSISEIVGVLTPTQSNLVYLVYGLAILALPFAILVGLIVARQWKLLTAY
                     AAAAFIAFVSLSITGNGISAPRWHFDLSDRLDTLLSQFLDDPRWIAMLAAVLTVSGPW
                     LPARWRRWWWTLLLAFVPIHLVVSAVVPARSLLGLAVGWFVGALVVLVVGTPALEVPL
                     DGAVRALARRGFLASTLTVVHPAGTGPLVLSAKSDGPDSVAIVEMYGPNQRSGGFLRQ
                     FWRWLRLRGGETAPLQASMRRAVEHRALMAIAIGDLGVASTSTTAMAPLDRGWTLYAH
                     TLPSGTPIKDCTDDGAVAQVWESLGTLHDCQISHGDLRSEEITIDDGTARFGGFGNAE
                     YGATDEQLQSDVAQLLLTTTEVYGAEAAVGAAIDVFGKDAVLLASRRLTKSAMPSRIR
                     KSVGDAGAVMSAARDEVKRQTGADQIKTETITRFTRNQLIQMVLLVALVYVAYPFIST
                     VPAFFSELRTANWWWALLGLAVSALTYVGAAAALWACADGLVSFKNLVIMQVANTFAA
                     TTTPAGVGGLALSTRFLQKGGLGALRATAAVALQQTVQVITHVALLIFFSAAAGATAD
                     LSHFVPSPTLLYLIAGLLLGLIGAFLFVPKLRRWLATAVRPRLKEVLSDLAELAREPK
                     RLSIIVLGCGTTTLGMALTLWASIEAFGGNTTFVTVTVVTMVGGTLASAAPTPGGVGA
                     VEAALIGGLAAFGAPTAIAVPSVLLYRILTCWLPVFIGWPIMRWLTDKDMI"
     gene            complement(13058..14509)
                     /locus_tag="VFC01_16400"
     CDS             complement(13058..14509)
                     /locus_tag="VFC01_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN81248.1"
                     /translation="MRPRSLLVRYAAGLAFAYLLSAGEVVAIVISVSGESVIGAQAVL
                     SRNNLIATVAIVIFGTFTVWLSGVALVARALHRAGPARTRKPPNITRRQSAILLTPWA
                     VAAAVLIPLNLDGGIRVLTLLVSAVMFGATATVCTGFLFTQRALRALMIAATVDLEHA
                     ERAPGVRARLILMWTMCTALPGAGIVVLVMLRANGWIIQPDAPIETPVLVLALVAVVL
                     GLRAMILVSSSISDPVHEVVDAMAEVERGQLGNKIDVYERSELGQLQSGFNRMVAGLQ
                     ERDRLRDLFGRHVGEEVVRRAVEDHESVAEDEREVAILFIDLVGSTQLATRREPHEVA
                     AVLNDFFRIVVAQVDKRDGLINKFQGDAALAVFGAPLRIENPASAALATARALGEELQ
                     RLLVDFGIGVSAGPVFAGNIGAENRYEYTVVGDAVNEAARLADRAKDFDRRVLCSEAA
                     IERADEAERRCWALQGSEMLRGRSQLTQISSPV"
     gene            complement(14522..14824)
                     /locus_tag="VFC01_16405"
     CDS             complement(14522..14824)
                     /locus_tag="VFC01_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81249.1"
                     /translation="MIKNGTRLQSQVCDTQVIVVRSADSLDDLRAGGFPMVPLDAEKS
                     SDATLDPDFAEGNVMGKRYVDENGAEVLVTKAGAGTLSVGTTALNLKEAKPLPASD"
     gene            complement(14821..16314)
                     /locus_tag="VFC01_16410"
     CDS             complement(14821..16314)
                     /locus_tag="VFC01_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN81250.1"
                     /translation="MSISLLLEMAASSNPDRTAVVSGEIRLTTSELSELADGGAGVIA
                     ASGAQHAAYVGTGGVLLPLLLFSSARAGVAFTPLNYRLSEDGLRRLIDRLPTPLVIAD
                     AEYRDVVADAGKQVITSDEFIDAARNAEPAAEFPDPDDVGVVLFTSGTTSKPKAVELT
                     HNNLTSYVMGTVELDSAESSDAALICVPPYHIAGVSAALSNLYAGRKMVYLRQFDPSE
                     WVRLVRDEGVTTATVVPTMLDRIVSVLEAQQVDLPTLRNLAYGGSKVALPLVRKALGL
                     LPDVGFVNAYGLTETSSTIAVLTPDDHRAALDATDEAIARRLGSVGQPVPSVEVQIRG
                     EDGTVLGPGQTGELFVRGEQVSGRYTDIGSVLDADGWFPTKDVAMLDEGGYLFIGGRS
                     DDTIIRGGENIAPAEIEDVLVEHPAVRDCVVVGPEDPEWGQIIVAVVVPAASTAPDPD
                     ELREHVRAHLRGSRTPDRVVFRDELPTNATGKVLRRELVDELQRSRT"
     gene            complement(16311..17129)
                     /locus_tag="VFC01_16415"
     CDS             complement(16311..17129)
                     /locus_tag="VFC01_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN81251.1"
                     /translation="MSTFTDRTLVVSGGSRGIGLAIALGAAKHGANVVLLAKTAEPHP
                     KLPGTVYTAVADVEAAGGKGVAVVGDVRKEDDVQRAVDTAVEHFGGVDIVINNASAIA
                     TDPTEELSAKKFDLMMDINVRGTFLLTKAALPHLRKSANAHVVTLAPPLNMNPYWLGA
                     HPSYTLSKYGMTLLSLGWAAEYADANIGFASLWPETYIATSAVANLDSHLMSSSRSPE
                     IMGDAAVEILSRSPADVNGKTFIDSAVLAEAGVTDLTRYGGGDNPILDIFVDKP"
     gene            17320..18897
                     /locus_tag="VFC01_16420"
     CDS             17320..18897
                     /locus_tag="VFC01_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZN81252.1"
                     /translation="MSDPVQTPSGIPLEPVYGPADRAGDPPAPGTYPFTRGNFASGYR
                     GKTWTFRQYSGFGTAEESNRRYRYLLDQGGTGLSVALDLPTQCGYDSDDPEFGEEVGR
                     VGVAVDTLADAEILFDGIPLDKISTSFTINGTAAILLAFYVAAAEKKGVPRAKLTGTI
                     QNDILKEYASRGTWIWPPEPSLRLIADTIEFCAAEVPKFNAISVAGAHFRDAGANAVQ
                     EMAFTLADGVTYCDTVVERGRMTIDQFAPQISFFFYTHGDFFEEIAKYRAGRRRWATI
                     VRERYGATTDKASMFRFGCVAGGASLYAPQAQNNLVRVAYEAMAAVLGGVQSMFTAAW
                     DEPFALPSEESATLALRTQQILACETGVTKVADPLGGSYFVEALTDATEEKIIAIMHD
                     LETHGGMVCAIEDGYLQGLIADEAFKIHQEVESGERPVVGVNKFVSDEPPPEIATYEL
                     DAEGRDLQLKRLSKVKAERHPAAVKDALAALSRAAEGDDNLMHKLIDCANAYCTVGEM
                     VSALKAVWGEFQQPVVF"
     gene            18897..19295
                     /locus_tag="VFC01_16425"
     CDS             18897..19295
                     /locus_tag="VFC01_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HZN81253.1"
                     /translation="MATRVLVAKPGLDGHDRGAKIVARTLRDAGFEVIYTGIRQRIED
                     IVSIALQEDVALVGLSILSGAHVALTTRTVEALRAADAGDIAVVVGGTIPQGDVPKLL
                     DAGAAAVFPTGTPLDVLVDEVRKLTSERVD"
     gene            19295..>19829
                     /locus_tag="VFC01_16430"
     CDS             19295..>19829
                     /locus_tag="VFC01_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN81254.1"
                     /translation="MRLGVMIGAERGDMARKVKKLLDDIEWAEGAGLDTAWMPQVPND
                     FDCLTMVSLMGARTSRIELGTAVVPLQAQHPIALARQALSVHAGTGGRLALGVGPSHH
                     WIVRDMLGIPYEKPAAYTRDYLEVLNTALAGPGEVDVENESFTVHNPTVLGADPPMPV
                     LVAALGPVMLQIAGELAD"
BASE COUNT         3561 a         6310 c         6437 g         3521 t
ORIGIN      
        1 cgccatgtgc ttgtcgaaga actccgcgaa gctctgggcg ttctggccgt cggcgaccgt
       61 ggcgttgcga gctactgcgg tcttgattcc accgggatgc acgcacgtca cctttaccgg
      121 atgcttggcg accaacatct cttggcgcaa cgcctcggtg aatccccgca ccgcgaattt
      181 cgcggcgttg taggcatttt ggccgggcac agcgatcaac ccgaacaaac tggatatgtt
      241 gacgacgtgt ccgtccccgg acacgatgac gtgggggaga aaggctttgg aaccgttcac
      301 gactccccag aagtcgacgt ccatgatccg ttcgatgtct ttgaactcgg acttctcgag
      361 gtcgccgttg taggcgatgc cggcgttgtt gtagacctgg tggacctcac cgaagtgggt
      421 gacgacggcg tcggcgtagg ccagcacggc ctcccgctcg gcgacgttga gccggtcgga
      481 tttcactgtc gcgccgagtg attcggcctg gcggacggtt ccggcgagtc cttctgtgtc
      541 gatgtccgag agcgcgagct tggcgccctt cgtcgcgaga ttgacggcta gggctctgcc
      601 gatgccggat ccggctccgg tgatgacggc gactttgcct tcgaatcggt tcatttcact
      661 gcaactttca cgttgttggt gtggacgtgg ttgtggagct tcgcgccgag atcggcgacg
      721 gcttcacgcg cttcggcgag cacgctgacc tgaaggtgaa agacgtgcca aaggttttcg
      781 aaaacccgat gatcgacggt gggggtgccc gcgacggcca cggccctggc aatgtcgagg
      841 gcatcgcagc ggatcggatc gtcaccggcg gtctgcatga cgatcggcgg caggccggtc
      901 atatctccgt agaccggcga aatgcccggc gacatcggat cggcggtccc cgcatactgc
      961 ggagcccatt cggccgtcat cgtcggcagg atcaacggat cccgcaattt cggacgtaac
     1021 tggtcgatgt cgatcgcgag gttggcccaa gggcagatca agcccagcgc cgccggcgcg
     1081 gcgatcccgc gatcgcgcag tgccatggcg agtgccagcg tcagtccgcc gccggccgaa
     1141 tcaccggcaa cgaccgtgct tttcgggtcg aggccgtctt caagtagtcg cgagtacacg
     1201 gcgaccatct cttcgagggc gaccggatat cgagcttccg gtgcgcgtga gtagtccggg
     1261 atgatcgccg tcatgccgga agccgcggcc aggtgcgcga ccaggccgcg atagcccttg
     1321 gcgctaccca gcgcgtagcc tccgccgtgc aggtagatca gtgttccgtt cgtcccacct
     1381 agcgggggct gtacccgctc cacggcaacg tccgcgatgg tgactcggtc gaaggtcgtg
     1441 cctttcgggg ggcgggcggc acccgtgagc atgttgacca tggcgcgctt gccgttgacc
     1501 gaaacccggt gcgaggcgaa cagtggtcgc gaggctcgta ggccggcccg gatgacgcgc
     1561 ttattgatgt tcatgatggc ctttcactaa caagttgatg gcgaaatgtg ttggggcggc
     1621 cttgttcaga ccgcgaccgc gtcgacggtt gtcttgtcga actgcagctg gttgtcctcg
     1681 accggatcct ggagcagcat cttggagtcc ttgccgtagt gcatgagcac cttccacggg
     1741 tacttggacc cctggcgcgg cagcgtgtcc tttgcgcgct gcacgtatcc ggattgcagc
     1801 tgatcgagca tgccgacgtc catcgcggag ttatcgatat cgaggggagt cgccaccgtg
     1861 tgaccgttgt cgtccatgtg cttgaacagg cggcacaggt agaggcctgc gatatcggac
     1921 ttcaaggtcc acggcgcgtt ggtgtagccg aaaacccagg ccaggttggg gatgttctcg
     1981 acgagcacac ccttgtaggt catctggtcg ttgagcttgc gcgcctcgcc gtcgaccgac
     2041 agctccatgc cacccagcat ctggatgtcc aggccggtcg ccgcgacgat ggtgtctgcc
     2101 tccagttcgc gtccggactt cagcaggatg cctttctcgg tgaaggtctc gatgtgatcg
     2161 gtgacgatcg acgcttcacc ggaggcaagc accttgaaga ggtcgccgtt cggcaccgca
     2221 cacaaccgtt cgtcccacgg catgtactta ggggtgaagt ggctcatgtc gacggacggg
     2281 ccaacgtgcc tgcggacctg ccagagcagg aatcggcgca tctgcgcggg ccagcgcttg
     2341 catgccaggt agaggtaacg ctggatggcg atgttgcgct tgcgcgcaaa cttgtagacc
     2401 cacttgtccg gcaggaatcg acctaggact tgggagatct tgtcgagggc gggcaccgag
     2461 aagatgtacg acggtgagcg ttgcagcatg gtcacgtgct cggcatcgcc ggccatcgtc
     2521 gggaccaggg tgacagccgt tgcgccactt ccgatcacga cgaccttctt gccggtgtag
     2581 tccagatcct cgggccaatg ttgggggtgg atgaaccgac ccttgaattg gtccgcgccg
     2641 gggaacgttg ggaggtagcc ggcgtcgtag ttgtagtagc cggtgcagct gatgaggaag
     2701 ccggcggtgt aggtgcgcgt ctcgccggac gcctcgtgca gcgctgtcac cgtccagcga
     2761 ctgtcggggg tggaccagtc ggctgtgacg atcttgaggc cgtagtggat cttctcgtca
     2821 atgccgtact ccgccgcggt gtcggcgatg tactggcgga tcgacgcgcc gtcggcgagc
     2881 accttcagtt cctgccatgg ccggaacttg tatccgaagc tgtacatgtc ggagtccgaa
     2941 cggatgccgg gatagcggaa cagatcccaa gtgccgccca ggcgctcgcg tcgctccaac
     3001 accgcgatgg tcttgttcgg gtgctcggcg ctcacctggc aggcggtgcc gatgccggac
     3061 agcccggcgc cgatgatcag gatgtcgaaa tgttgagcgt tcatggcttc ctcgcgcttc
     3121 tggcctgctg cgttgcagga cgttgtagaa ccagaatcgt tgacgaggcc attgcaggtc
     3181 ttgactgttt ccgacatact tttgtcattt cccgacacgg gagttgatcg gatggtcagc
     3241 ctggttcgcg ccacgaacct gtggggatac ggcgacctcg ttcgcgagct cggtgccgac
     3301 ccgcaaccct tcctttcgcg tttcaacatc cagcccggtg tcgagtacga ggaagacgcg
     3361 ttcatcccgt tcgaggccgc cgctcgcctg ctggaggtca gcgcggacga actgaattgc
     3421 ccggatttcg ggcttcggct ttcgcgctgg cagggcatgg acatcctcgg accgatcgcc
     3481 gtgatcgcgc ggaacgctca taccgtcctc gacgggctgg agtcgatcgc gagttatctc
     3541 tatattcatt ccccggcgtt gaaactcacc gtcgcgtcgc ccaccgcggg cgccgacgtc
     3601 aggttcacct tcgaggtcgt cgaaccgggt ctgtcctacg tgctccaggc atacgaactg
     3661 agcttggcca acggcgcacg gatgattcgc cttatgggcg ggccggaggc acggccccgg
     3721 gcaatttcgt tcattcacga acaacggggc tcggatgcgg cgtactccga agcgttgggt
     3781 tgtcgcgtgc gcttcggaca gaagtggtgc ggcttcgacc tacccgagca tctggcaggc
     3841 aagcgcattg tgggtgcgga tcccgagacc cgacgcatcg ccaccaaata cctggagtcc
     3901 aactatctgc cgagcaccgc cacgctgtcc gaacgtgtgg ccgagctggc ccgacgactc
     3961 ctgccaacgg ggcaatgcag cgttgaagcc atcggcgatc aactggcgct gcatccgcgg
     4021 acactgcaac ggagcctggc cgccgaaggc gtgcgatgtc aggatctcat cgagcgcgag
     4081 cgccgcgacc aggccgcgaa atatctcgct gaaccgaggt tgcatctgag tcagatcgcg
     4141 actctgctgg gctacacgga acagagcacc ttcaaccggt cgtgtcgccg atggtttggg
     4201 aagacccccc ggcagtaccg agccgacctg tcggtatcgc cgtcgcgagt ccaccggtga
     4261 cttacgtgat tattgctccc gtgcaaccga tctcgagatc tcagtcggcc gcagcggcct
     4321 gtacttctgc cgcaagctgc cgatggcgtg tactccggtg atgaccagtg ggatgtgcac
     4381 cgggggagac aacgaggagc aatagcgcga cgagggtgag attggacgtg gttcgctcat
     4441 atttctccgc tagaagcggg tcggtttgga attcggtatg aagtagtccg tgtttcttgg
     4501 ttctcagcgg cgtttcgact tcgccgactt cagcagactg acctgcagtt ccagctcgtt
     4561 gaccagagcc tgtgccagaa gatcgacatc ggggaccagt tcgggatcag ccatgacacc
     4621 gatgtgcagt gtgtcgagat agctgaatag cgtgatgttc aaagccattc cgtcgacgat
     4681 cgtcgagacg ggatagttgg cgagtacgcg tgctccggcg caatagcgcg gtgtccgagg
     4741 accagggaca ttcgagatga tgacattcgc cgcagggttc agccttctcg aacttgcgag
     4801 ccgcagcacc gtccgcatcg ccggcccgaa cagcaccggc ggtacaaggt cgttggcatg
     4861 ctgcatgagg ttgggtggtt cggcctgctg ctgctgcttc gcctgctgta acgaatcacg
     4921 acatgcacgg atccggcctg cggggtcggc ctcgtcggtg ggtagcggaa cgaccatggc
     4981 agagaccttg ttgccgaaag tgcttcgttc ggccggtgtt cggaccgaga cagggaccat
     5041 ggcgagcagg cgctcgtctg gcaattcatc tttgccgtct agccagttcc gaagtgctcc
     5101 tgcacacagg gaaacgacga tgtcgttgac ggttgcaccg tgagcttcct tgagttgcaa
     5161 cacctctggc agaggaaggc tcaccatgac aaggctgcga ttcggcgtga tgcgggcgtt
     5221 gaatgcggtg cgcggagcga ctatcgccgg tgatgccgca gattcggcgg gactccccgg
     5281 cgcgaatcgc cctaccaagc ccgcgatctc gcctatcccg ggcagcgatc gaatcgtcgc
     5341 gactttgtcc aggtgggcta gcgctgggcg caccgcaccg acggcggcga gctgctggcg
     5401 gggcagatcc gccgagcctc gtaccagcat ccccaactga ttcgggacgg attcagtctg
     5461 ccggctggta gtaggcgcta cttcgcggcc gttgggtgac tcatcgagca gggcacccag
     5521 tatctcgccg cccgacaccc catcgacggc agcgtggtgc aacttgatca acagggcaac
     5581 ccggtcaccg gacaggccgt ggatcaggta cgcctcccag agcggccggg accggttcgt
     5641 cggttcggcg gccaggcggg ccacctgctc ggcgaggacg gcatcgtcgc cgggagccgc
     5701 cagcgcgaat tcccggacgt ggaagtcgag atcgactggg ccgtcgaccc atgacgggtg
     5761 cgcgacgccc aacgggactt ccgccaactg ccaccgcagc ggcgggagca ggtgcaggcg
     5821 ctgtgcgaac agtgttttga cggcatggcg tgtgagacgt cggccgttcg cccgctttcg
     5881 ttcgagtatc acgagggccg aaacgttggc gacggtgcgc tcatcctcgg tggcgagaaa
     5941 gaatgcgtcg aggctggtca ggcgtcgcat ggcggtttcc ttagttgctg tgtatgaact
     6001 gccggcgatg aacagccttg gggatcaagg tcttcgccaa acttctgttg acacaactgt
     6061 gcacgccacg ctgcccgggg gtcttggcac aaggcgacag gtttttagca ttttgcgact
     6121 gtcacggaac cgccgagact gcggggaacc gctacgcagt caccaccgtg gttgaggtcg
     6181 tcaccttttt cggactggaa atccgttgcg ctctgccaac gaccgcaact gcttcagcgc
     6241 gaaccggcgg ccccgaccag ccgccggtat cagcacgccg gcccagatgc cctccgcgcc
     6301 cggcgtctca caagcttcct gagcgcaccg ccagcggcgt gggcactcgc ggcacagcgc
     6361 cttggcgccc tcgtccgcga ccgtcgtcca tcggtctgga tcccgcgcac atacgagtgt
     6421 cccgaccccc gaattccgca ctcagctcgc ctttcgtttt cgcactcgac aatatgactg
     6481 tattgtagga gcgatacaga cgtattgtcg accgcgccga atgcaagggc ccttcacctg
     6541 tgtgtatgaa tgatggttgt gctgccgcag accgacgccg ttagcgcgga cgagcgctcg
     6601 agtatcgagc ggatccgtag cgcggcgttg cgaagcttcg ccacatacgg cgcttcggcc
     6661 acatcgctgc gcacggtcgc cgaagccgca ggcgtgtccg tcggcctcgt gcagcaccat
     6721 ttcaccaaca aggccggact gatcaaagcc gtcgatgacc atgtcatggc agtggtagtt
     6781 gagctcatcg cgcagccact tccggcgcca ccggcagact cgatcgccga agtgggcagc
     6841 agagtcaccc ggttcatgtc cggctaccca gacgtcgtcg actacgtcgg ccgcgcactc
     6901 atcgacggca gcccgctggg aaccacgatc ttcgacaccc tcgctgcctt cggcatggcg
     6961 cgatggagcc aacgcaaaga acgcggtgaa gtgcgtcccg atgtcgatct gacctgggcc
     7021 gccctcaact cgctcgtgct agctctgggc gcgatgattc tgcgcggcca tatcgaacgc
     7081 catctccctg agcccttcac tacgcccgcc cagctacaac gatggcaaga cgccgtcaat
     7141 aaattgctgc gcggaggcgc ggtctagaca cgcccgtcaa accctcgcgc cgccaaccct
     7201 tttgcgatac gtcgaaggtg tctctccgca gaactgtgtg aacgcgcgcg tgaacgcgct
     7261 caggctgtcg aagccgaccg ccgtcgaggt ttcctgcacc gactgtccgg gcgcggccaa
     7321 cagtgccatc gctcgcagca ttcgcgcatg caaaaggtac gtccgccacg gcagcccgag
     7381 cgtgtcctga aacagccgtc gcaatgtccg ctccgacacc gacacggcgc ggccgacctc
     7441 cgacgcggtc accgaatcca agtgctcctt cgtgtacgcc atcgccgccg ccacgatcgg
     7501 gttctccgac gtcggcaggc tcagtggcgc ctcgtgatcc agcgcctcgg acaccaggtt
     7561 ggccaatgtg cggaagaacg tgtcggagac ggcgtcgccg tcgggccggt cgatcggcca
     7621 tcgcagcgcg tagatcatca tctctcggat caacggcgac actgcgagga tgcgggcgcg
     7681 gtcgccgcca tcggcgacta actcgcggtc gaacatcacc gccactgtgc gaacgtccga
     7741 gttcatcgtg gcctgatgct ccaggcccgc gggaatccac gccgcctgct gcggcggcag
     7801 caggtagtgc gccgacgccg tctccacctc caccacaccg ccgatcgcgt actcgatctg
     7861 atgcacctca tgcgagtgcc agcccgtgat cagctcgtcg ccctcgtaga ggtaactgcc
     7921 gcccagcgcc cgcccgccac gccgcaactc gatgaccggc cgacgattgg ccgattcggc
     7981 taaaactctg tccgaaagcg cagagacggt cacaccgcta gacggtacta cttgggatat
     8041 gaagaccgac gacctgattc tgatgagcat cgacgaccac gtcgtagagc ctcccgacat
     8101 gttcctgaac cacgtgccgg ccaagtacaa ggacgaggcg ccgatcgtgg tcaccgacga
     8161 caagggcgtc gaccaatgga tgtaccaggg ccggccgcag ggcgtcagcg gactcaacgc
     8221 ggtggtgtcg tggccggccg aggagtgggg ccgcgacccc gccggcttcg ccgagatgcg
     8281 gccgggcgtg tacgacgtcc acgagcgcgt gcgcgatatg aaccgcaacg gcatcctcgc
     8341 gtcgatgtgc ttcccgacat tcaccggctt ctcggcacgg cacctcaaca tgcaccgcga
     8401 gcacgtcacg ctcgtgatgg tgtcggcgta caacgactgg cacatcgacg agtgggcggg
     8461 ctcgtacccg gatcgcttta tcccgatcgc cgtgctgccg acgtggaacc ccgaggcgat
     8521 gtgtgccgag atacggcgag tcgccgccaa gggctgccgc gcggtgacga tgccggaact
     8581 gcctcacctg gaaggacttc cgagctacca cgacgacgag tactggggcc ccgtgttcca
     8641 gactctgtcg gaggagaacg tggtgatgtg cctgcacatc ggcaccgggt tcggggcgat
     8701 cagcatggcg cccaacgctc cgatcgacaa tctgatcatt ctggcgactc aggtgtcggc
     8761 gatgtgtgcg caggatctgt tgtggggtcc cgcgatgcgc aactatcccg atctgaagtt
     8821 cgcgttctcc gaaggcggta tcggctggat cccgttctac ctggatcgca gcgaccggca
     8881 ctacaccaac cagaagtggc tacgccgcga cttcggtgac aagctgccca gcgacgtgtt
     8941 ccgtgagcac tcattggcct gttatgtcac cgacaagacg tcgctgaagc tgcgccacga
     9001 gatcggcatc gacaacatcg cgtgggaatg cgactacccg cactccgact gcttctggcc
     9061 cgacgcgccc gagcaggtgc tggctgagct caccgccgcg ggcgccgacg acagcgacat
     9121 caacaagatc acgtggggga actcctgccg gtttttcagc tgggatccat tcgcccgcac
     9181 tcccaagaac caggcgaacg tcggtgcgct ccgcgccaag ggggccgatg tcgacgtgtc
     9241 gatcaggccg cgtagggagt gggcgcggct gtacgcggaa aaacagctcg ccaaggccta
     9301 atgatccgca tttgcggaag aaattattcg attagaccgc atctgcggtt aagatgccgc
     9361 gatgggtttg atccgagtcc gggaagcggc cgagctgttg ggcgtcagcg acgacaccgt
     9421 ccggcgctgg atcgacgacg gaagcctgcg ggcgagccac gacgaatcgg gccggaaggt
     9481 catccccggt gacgtgctag ccaagttcgc gcgtgcaaac cctgctgcgg caccgaagga
     9541 tccgctcacc gtcgccagct ccgcgcgcaa ccgcttcgcc ggactggtca ccaaagtagt
     9601 gacggacaag gtcatggcac aggttgaaat gcagtgcggg cctttcaccg tcgtttcgtt
     9661 gatgagcacg gacgcggtgc gtgagctcgg gctcgagccg ggcagcgtgg ccgtcgccgt
     9721 cgtcaaggcc accaccgtga tcgtcgaaac tcccggaggg caatcgtgaa acgttggctg
     9781 atcgccgcgc tgtccgtgct tctgatcggt gggtgcggct cgccggaaaa acccgcagct
     9841 gaaaatcaga agatcatggt gtttgcggcc gcgtcgctga agaagacctt caccgacctc
     9901 ggggagcagt tcaagaccga aaaccctggc gcctcagtgg aattctcgtt cgcgggctca
     9961 tccgatctgg ttacccagct gacccagggt gcgcaggccg acgtgttcgc atcggccgac
    10021 accacgaaca tggacaaagc cactcaggcc ggggtgctcg ccggcgaccc ggtgaacttc
    10081 gcgtccaaca ccctcactat cgccgtcgcg cccggcaacc cgaagaagat cgcgtcgttc
    10141 aaagacctga cgcagcaagg cctcaacgtg gtggtgtgcg caccacaggt gccgtgcgga
    10201 tcggcgaccc agaaggtcga gcaggccacc ggcgtgacgc tcaatccggt cagcgaagag
    10261 tcgtcggtgt ccgacgtcct caacaaagtc actaccggcc aggccgacgc cggactggtc
    10321 tacgtcaccg acgccaaagg tgcgggcgac aaggttgccg ccgtggcgtt ccccgaagcc
    10381 gccggcgccg tcaacaccta tccgatcgct gtgctgaaga gctccaagaa ttcggagttg
    10441 gcgcgcaagt tcgtcgacct cgtcaccggg gagtcgggtc aaaaggtcct cgacgcagcg
    10501 ggtttcgcca aaccctagat catgtccttg tcggttagcc agcgcatgat cggccagccg
    10561 atgaacacgg gtagccagca cgtgaggatc cggtagagca ggaccgatgg cacggcgatt
    10621 gcggtgggag cgccgaacgc ggcgaggccg ccgatcagag ccgcctcgac tgcgccgacg
    10681 ccgcccgggg tgggggcagc cgaggcgagg gttccgccga ccattgtcac cacggtgact
    10741 gtgacgaacg tggtgttgcc gccgaaggcc tcgatgctgg cccatagcgt cagcgccatg
    10801 ccgagagtcg ttgtcccaca gcccagtacg atgatcgaga gccgtttggg ttcgcgggcc
    10861 aactcggcga ggtcgctgag cacttccttg agccggggcc gcaccgccgt cgccaaccac
    10921 cgccgcagtt tgggcacgaa cagaaaggcc ccgatgagtc cgagcaacag tccggcgatc
    10981 aggtagagga gagtgggaga ggggacgaaa tgggaaaggt ccgccgtggc tcccgcagcc
    11041 gcactgaaga agatcagtag cgcgacgtgg gtgatgacct gaaccgtttg ttgcagtgcg
    11101 acggccgccg tcgcacggag cgcgcccagg ccacccttct gcaggaagcg ggtggacaac
    11161 gccaacccgc cgacaccggc aggcgtggtg gtcgctgcaa acgtgttcgc cacctgcatg
    11221 atgacgaggt ttttgaagct caccaacccg tcggcacacg cccacaacgc cgccgcggca
    11281 ccgacatacg tcaacgccga caccgcgagg ccgagcagcg cccaccacca gttcgccgtt
    11341 ctcagctcgg agaagaacgc gggcactgtg ctgatgaacg ggtaggcgac gtagacgagc
    11401 gcgacgagta gcaccatctg gatgagctgg ttgcgggtga accgggtgat cgtctcggtc
    11461 ttgatttgat cggctccggt ctgccgcttg acctcgtcgc gggcggcgga catgacggcc
    11521 ccggcatcgc ccaccgactt gcggattcga gatggcatgg cggacttggt cagtcgtcgc
    11581 gatgccagca ggaccgcatc cttgccgaag acgtcgatcg ccgcgccgac cgccgcctcg
    11641 gccccgtaca cctcggtcgt cgtcagcaag agttgggcga cgtcggactg aagctgctcg
    11701 tcggtggctc cgtactcggc gttgccgaac ccgccgaacc gcgctgtgcc gtcgtcgatg
    11761 gtgatctcct cactgcggag gtcaccgtga gagatctggc aatcgtggag tgtgccaagc
    11821 gattcccaga cctgggcgac ggcaccatca tctgtgcagt ccttgatcgg ggtgccggat
    11881 gggagagtgt gggcgtacaa cgtccatccg cgatcgagtg gagccatggc cgtcgtcgag
    11941 gtactcgcga cccctaaatc gccgatcgcg atggccatca acgcccgatg ctcgacggcg
    12001 cgccgcatgg aggcctgtag cggcgcagtt tcgccaccgc gcagccgcag ccaacgccag
    12061 aactggcgca ggaatccgcc gctgcgctgg tttgggccgt acatctccac gatggccaca
    12121 ctgtcggggc cgtcagactt ggctgacaac accagcggcc ccgtcccggc tgggtgcacg
    12181 accgtcagcg tcgatgccag gaatccacgc cgggccagcg cgcggacggc accgtcgagc
    12241 ggcacctcga gggcaggcgt gccgaccacg agcacaacga gcgcgccgac gaaccacccg
    12301 acggcgaggc cgagcagcga ccgagcgggt accaccgcgc tgacgacgag gtggatcggc
    12361 acgaaggcga gcagcagcgt ccaccaccac cgccgccacc gcgcgggcag ccacgggccg
    12421 gacaccgtga gcacggcggc gagcattgcg atccagcgcg ggtcgtcgag gaactgcgag
    12481 agaagtgtgt ccagccggtc ggacaggtcg aaatgccatc tgggagcgga gatgccgttg
    12541 ccggtgatcg acagcgaaac aaacgcgatg aacgccgcgg ccgcgtacgc ggtgagcagc
    12601 ttccactgcc gcgcgacgat caagccgacc aggatcgcga acggcagcgc cagaatcgcg
    12661 aggccgtaca ccagatacac cagattcgac tgagtggggg tcaggactcc gacgatctct
    12721 gagatcgatt tctcgagtgc gacccattcg ttgcgggtga tcagcgaact cgtgatgacc
    12781 gccgccagga aaaccgccgc aaggacgact cgcaggatgt cgttggtccg ccgggtcagt
    12841 ggttgcagca agctgcccga aacggtgatg tcccgcccgt cgacccgcat gtcccagcat
    12901 ccttgcacgt cgtcatgcat gtgggcggta gcttggcgcc atgagtggag tggtggtcac
    12961 gggcgaggcg ttcggaatcg ggcgagctcg gtgatttcgg cgcgctaacg gtcgctgagc
    13021 gacaggtcgc tgagcgaccg tatccgcgcc gaaatcacta cacaggcgac gagatctgtg
    13081 tcagttgcga ccgtccgcgc agcatctcgg agccctgaag ggcccagcat cgccgctcgg
    13141 cctcatcggc ccgctcgatc gccgcttcgg aacacagcac ccgtctgtcg aagtctttgg
    13201 cgcggtccgc cagccgggcc gcctcattga cggcatcgcc gacaacggtg tactcataac
    13261 ggttttccgc tccgatattg ccggcgaaca ctgggccggc cgagactccg atgccgaagt
    13321 cgacgagcag ccgctgtaac tcctcgccca atgcccgcgc ggtggccaac gccgccgacg
    13381 cgggattttc gatgcgcagc ggtgcgccga acaccgccag cgcggcgtcg ccttggaact
    13441 tgttgatcaa gccgtcgcgt ttgtcgactt gcgcgaccac gatgcggaag aagtcgttga
    13501 gcaccgcggc gacctcatgc ggttcgcgcc tcgtcgccag ttgcgtagag cccaccagat
    13561 cgatgaacag gatcgcgacc tcgcgttcgt cctccgcgac cgactcgtgg tcctcgacag
    13621 cgcgacggac cacttcttca ccgacgtgac ggccgaacag gtcgcgtaac ctgtcccgtt
    13681 cttgaagtcc ggcgaccata cggttgaaac cgctttgaag ttggccaagt tcagaccgct
    13741 cgtaaacgtc gatcttgttc ccgagctgtc cgcgttcgac ctccgccatc gcgtcgacga
    13801 cctcgtgcac cggatccgaa atcgacgacg acaccagaat catcgcccgc agtcccagga
    13861 ccaccgcgac gagtgcgagt accaacaccg gggtttcgat tggcgcgtcg ggctggatga
    13921 tccagccgtt ggctcgcaac atgacaagta ccacgatccc cgcacccgga agcgcggtgc
    13981 acatcgtcca catcaggatc agacgcgctc gtactcccgg tgccctctcg gcgtgctcca
    14041 aatccactgt ggcggcgatc atcagcgcac gaagagcgcg ttgtgtgaac agaaagcccg
    14101 tgcacacggt cgccgtggcg ccgaacatga ctgccgaaac gagcaatgtc agaacgcgta
    14161 tgccgccgtc gaggttcaac ggaatcaaca ccgcagctgc gacggcccat ggcgtcagca
    14221 ggatcgcgga ttggcgcctg gtgatattcg gtggcttccg ggttcgagcg gggcccgctc
    14281 ggtgaagtgc gcgagcgacg agtgcgacgc cgctcagcca tacggtgaag gttccgaaaa
    14341 tgacgatcgc cacggtggca atcagattgt ttctgctcag cacggcctgc gcacctatga
    14401 cggactcgcc gctcaccgag atgacgattg cgacgacttc tccggcggag agcaggtagg
    14461 cgaacgcgag gcctgccgcg taccgaacca gcaggctgcg tggcctcatc aggccatatt
    14521 gtcagtcgct ggcggggagc ggcttggctt ccttcagatt caatgctgtc gtgccgacgc
    14581 tcagtgttcc tgcgcccgcc ttggtgacga gcacctcggc gccgttctca tcgacgtaac
    14641 gcttgcccat cacgtttccc tcggcgaaat ccggatccaa ggtggcgtcg gaagatttct
    14701 cggcgtcgag cggcaccatc gggaagccgc cggcacgcag atcgtcgagg ctgtcggcgc
    14761 tgcgcacgac gatcacttgc gtgtcgcaga cctggctctg cagccgggtg ccgttcttga
    14821 tcatgttcga ctcctttgca attcatcgac cagttcgcgg cgcaacacct tgcccgtcgc
    14881 attggtcggc agctcatcgc ggaacaccac gcgatcgggc gtgcgtgaac cgcgcagatg
    14941 cgctcgcaca tgctcgcgca gttcgtcagg gtctggtgca gtgctcgcgg cgggcaccac
    15001 gacggcgacg atgatctgac cccattcggg atcctccggg ccgacgacca cacagtcccg
    15061 caccgcggga tgctcgacga gcacgtcctc gatctccgcc ggtgcgatgt tctcgccgcc
    15121 gcggatgatc gtgtcgtcgg aacggccacc gatgaacagg tagcctccct cgtcaagcat
    15181 cgcaacgtct ttggtgggga accagccgtc ggcgtcgagc accgaaccga tgtcggtgta
    15241 gcgtcccgac acctgctcgc cacgcacgaa cagttcaccg gtctggcctg gcccgaggac
    15301 ggtgccgtcc tcgccgcgga tctgcacctc gacgctgggt actggctgtc cgaccgaccc
    15361 gagccgccga gcgatagcct catcggtggc gtccagcgcg gcgcggtggt cgtcgggggt
    15421 cagcaccgcg atggtcgagc tggtctcggt caatccgtag gcattgacga agccaacatc
    15481 ggggagcagg ccaagcgcct tgcgcaccaa gggaagagcg accttcgagc cgccataggc
    15541 caggttgcgc agggtaggca ggtctacctg ctgagcctcc agtaccgaca cgatgcggtc
    15601 cagcatggtt ggcacgacgg tcgcggtagt cacgccctcg tcgcgcacca ggcgcaccca
    15661 ctcgctcggg tcgaactgcc tgagatagac catctttcgg cccgcataga gattcgacaa
    15721 tgcggcgctg acgcccgcga tgtggtaagg cggcacacag atcaacgcgg cgtcactgga
    15781 ctccgcggag tcgagttcca cagtccccat cacgtaactg gtcaggttgt tatgggtcag
    15841 ctcaacggct ttgggcttcg aggtggtacc tgacgtgaaa aggaccacgc cgacgtcgtc
    15901 ggggtcgggg aattcagcgg caggctcggc gttgcgcgcg gcatcgatga actcgtcgga
    15961 ggtgatcact tgcttgccgg cgtcggcgac gacatcgcgg tactcggcgt cggcgatcac
    16021 cagcggagtc ggtaggcggt cgatcaaccg ccgcaggccg tcctcactga gccggtaatt
    16081 cagcggcgta aacgccactc cggcgcgcgc ggacgaaaac agcaaaagcg gcagcagtac
    16141 gccgccggtg ccgacgtagg ccgcgtgttg ggcgccggac gccgcgatga cgccggcccc
    16201 accgtcggcg agctcgctca gttcacttgt cgtcagccgg atctcaccgg acaccaccgc
    16261 ggtgcggtcc ggattcgatg acgccgccat ttccagcagc aaagagatac tcatggcttg
    16321 tcgacgaaga tatcaaggat gggattgtca ccgccgccgt agcgggtcag gtcggtgaca
    16381 ccggcttcgg ccagcactgc ggagtcgatg aaagttttgc cgttcacgtc cgcgggcgac
    16441 cgggacagaa tctcgaccgc ggcgtcgccc atgatctcgg gactgcgcga cgaggacatc
    16501 aagtggctgt ccaggttggc caccgcggac gtcgcgatat aggtctccgg ccacagggag
    16561 gcaaagccga tattcgcgtc ggcgtactcg gcggcccagc cgagcgacag caacgtcatc
    16621 ccgtatttcg acaacgtgta tgacgggtga gcgcccagcc agtacgggtt catgttcagc
    16681 ggcggcgcca gggtcactac gtgggcgttc gccgactttc gcaggtgcgg cagcgccgcc
    16741 ttcgtcaaca ggaaagtgcc gcggacgttg atgtccatca tcaggtcgaa cttcttggcg
    16801 gaaagctctt cggtcgggtc ggtcgcgatc gcgctggcgt tgttgatcac gatgtcgacg
    16861 ccgccgaagt gctcgaccgc ggtgtcgacg gcacgctgta cgtcgtcttc cttgcgcacg
    16921 tcacccacca ccgctacgcc cttgccaccc gcggcttcga cgtcggcgac ggcggtatag
    16981 acggtgccgg gcaacttggg gtgcggctcg gccgtcttgg ccagcagcac gacgttggcg
    17041 ccgtgtttgg cggcccccag cgcgatcgcc agcccgatgc cgcgactacc gcccgagacg
    17101 acgagcgtgc ggtcggtaaa cgtagacatg ggcctcctcg ggtgccggct acccggatgc
    17161 tacaggattg gaattgtcat tctcattcta cgcaagtctc ataatcgctg tgtaccgggc
    17221 cttcatgggc cgctcggtgc acaacgcagg ccgcactcag tgcatgtttc caccgagcgg
    17281 tattggcatt ctcatttttt gcaagtacgt tatccattga tgagcgaccc tgtacagacc
    17341 ccttcaggta tcccgctcga gcccgtctac ggaccggccg atcgggccgg tgatccgcct
    17401 gcaccgggca cgtacccgtt cacgcgcggc aacttcgcca gcgggtaccg cggcaagacg
    17461 tggacgttcc ggcagtactc cggcttcggg accgccgagg aatcgaaccg tcgctaccgc
    17521 tacctgctgg accagggcgg caccgggctc tcggtcgcgc tggatctgcc cacccagtgc
    17581 ggctacgact ccgacgatcc ggagttcggt gaggaggtcg gccgcgtcgg ggtggcggtc
    17641 gacacgctcg ccgacgccga gattctgttc gacggaatcc cgctcgacaa gatcagcacg
    17701 agcttcacga tcaacggcac ggccgcgatc ctgctggcct tctatgtggc ggcggccgag
    17761 aagaagggcg tgccgcgcgc gaagctgacc ggcaccatcc agaacgacat cctcaaggag
    17821 tacgcgtcgc gcggcacctg gatctggccg ccggaaccgt cgctgcggct gatcgccgac
    17881 accatcgagt tctgcgccgc cgaggtgccc aaattcaacg cgatctccgt ggcgggtgcg
    17941 cacttccgcg acgccggcgc caacgccgtc caggagatgg cattcacgct cgccgacggt
    18001 gtcacgtact gcgacaccgt ggtcgagcgg ggtcggatga ccatcgatca gttcgcgccg
    18061 cagatctcgt ttttcttcta cacccacggc gacttctttg aggagatcgc caagtaccgg
    18121 gccggccggc ggcggtgggc cacgatcgtg cgcgaacgct acggcgcgac tacggacaag
    18181 gcgtcgatgt tccggttcgg ctgcgtcgcc ggtggtgcgt cgctgtacgc gccacaggcg
    18241 cagaacaacc ttgtccgggt ggcgtacgag gcgatggccg cggtgctggg cggggtgcag
    18301 tcgatgttca cggccgcgtg ggacgagccg ttcgcgctgc ccagcgagga gtccgcgacg
    18361 ctggcgctgc gcacgcagca gatcctcgcg tgcgaaaccg gcgtgacgaa ggtggctgac
    18421 ccgctgggcg gttcctactt cgtcgaggcg ctcaccgacg ccaccgagga gaagatcatc
    18481 gcgatcatgc acgatctcga gacgcacggc ggcatggtgt gcgccatcga ggacggctat
    18541 ctgcagggcc tcatcgcgga cgaggcgttc aagatccatc aagaagtcga atcgggcgag
    18601 cgtcccgtcg ttggcgtcaa caagttcgtc tccgacgaac cgccgcccga aatcgcaacg
    18661 tacgaactgg acgccgaggg ccgcgacctt caactgaagc gactgtccaa ggtcaaggct
    18721 gaacggcatc ccgccgccgt caaggacgcc ctggccgccc tgtcccgcgc cgcggaaggc
    18781 gatgacaacc tgatgcacaa gctgatcgac tgcgccaatg cgtattgcac cgttggcgaa
    18841 atggtgtccg cgctcaaagc agtctggggc gagttccagc agccggtggt gttctagtgg
    18901 ctactcgcgt tcttgttgcc aaaccaggtc tcgacgggca tgaccgcggc gcgaagatcg
    18961 ttgcgcgcac actgcgcgat gcgggttttg aggtgatcta cacaggtatc cggcagcgca
    19021 tcgaggacat cgtgtcgatc gcactgcagg aagacgttgc gctggtagga ctttcgatcc
    19081 tgtccggagc gcacgtggcg ctgacgaccc gcaccgtcga ggcgttgcgg gcggccgacg
    19141 cgggagacat cgccgtggtg gtgggtggca ccatcccgca gggcgatgtc ccaaaattgc
    19201 tggacgccgg tgcggccgct gtctttccga cgggtacacc gttggacgta ttggtggatg
    19261 aggtgcgcaa gctgacgtcc gagagggttg actgatgcgc cttggcgtga tgatcggggc
    19321 cgagcgcggc gacatggcgc gcaaggtcaa gaagctgctc gacgacatcg aatgggccga
    19381 gggtgcgggc ctcgacaccg cgtggatgcc gcaggtgccc aacgacttcg actgcctcac
    19441 catggtgtcg ctgatgggtg cgcgcacgtc gcgcatcgag ctcggcacgg ccgtggtgcc
    19501 actgcaggcg cagcatccga tcgcattggc gcgccaggcg ctgtcggttc atgcaggcac
    19561 cggcggacgg ctggcgctgg gcgtggggcc gtcacaccac tggatcgtgc gcgacatgct
    19621 cggcatcccg tacgagaagc ccgccgcata cacccgcgac tacctcgagg tgctcaacac
    19681 cgcgctggcc ggacccggcg aggttgatgt ggagaacgag tcgttcaccg tgcacaaccc
    19741 gaccgtgctt ggcgccgatc cgcccatgcc ggtgctggtt gccgcgctcg gtccggtaat
    19801 gctccagatc gcaggcgaac tcgccgacg
//
LOCUS       DASRJA010000370         2819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3045456,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000370 DASRJA010000000
VERSION     DASRJA010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3045456"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..1253)
                     /locus_tag="VFC01_16435"
     CDS             complement(270..1253)
                     /locus_tag="VFC01_16435"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZN81255.1"
                     /translation="MPELVSKRAQVARIRRTAVRALADYAVDEPRLSFIAHGENTTFR
                     VDSRDARFLMRVHRPNRHGRAVDSRVAVGSELAWLAALQADTDLSVPTPIRACSGQWT
                     TVADGRVCSVLGWQNGRMQAANPRSVHFRRLGGILARLHDHASVWAPPSGFVRMRWDW
                     ETFFGNTMEYGGVSAAGCWDLLPVPLRAQFDEVARRMRTVMAGLGSKPDAFGLIHADL
                     HLENALFDGDAVRLIDFDDCGFGYWLYDIAVPLWEYRGRADYAAIRAALLNGYGERRE
                     LPDLTHLDDFIATRDVAFGLWFAGMAQVNPAFAADLDKVMDSIQRSLDRVL"
     gene            complement(1455..2114)
                     /locus_tag="VFC01_16440"
     CDS             complement(1455..2114)
                     /locus_tag="VFC01_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81256.1"
                     /translation="MSAAFAGTRSSTRVLVLVGVVWFALAYIAAAVGLVDRPATEPPL
                     RIALAAGIPVLAVAVGLLTSRRLRWFAATLDLPLLINLQALRAAGFAFLVLYAADLLP
                     GGFAFPAGLGDLAVALTAPFVARYVVRRTRAAWPVFLGWTLFGIADFIMAVALGAIHD
                     LGAQRTAADATIITPPMAELPLSLIPTFAVPFLLVVHLLAIARFRADETTQDPSPNTS
                     T"
     gene            complement(2289..2537)
                     /locus_tag="VFC01_16445"
     CDS             complement(2289..2537)
                     /locus_tag="VFC01_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81257.1"
                     /translation="MRRPVVVNDGDLDPSPQFAEDLAAQLAFAGFDGGTRCRGAGRLA
                     RSCQLRPYEDRRGRTAAARVHGFPLLAESSERADNGCA"
BASE COUNT          503 a          921 c          977 g          418 t
ORIGIN      
        1 cgtagctgag tgttctggaa gcgcgctgct tgctgctcca gcacagctgg atggtgtggg
       61 cgcggcttgg cgggttgagg cttcacgttc cgttcaacta gatcaacaca gacatgacgc
      121 ggtgcttcgg gagctatttc ccagcgcccc acccatgatc actactggcc gcggccgcgc
      181 ttatccgccg gcgctgtgga ttgggtcacc tgtgtgggga atggtggccc ggctttccac
      241 ccgaactgcg ggtctgaacg aggacacagt cacagcactc ggtcaagtga ccgctggatg
      301 gagtccatca ccttgtccag gtcggccgcg aatgccggat tgacctgagc catcccggcg
      361 aaccacaggc cgaaggccac gtctcgagtg gcgatgaagt cgtccaggtg agtgagatcg
      421 ggcagctcgc ggcgttcacc gtagccattg agaagcgccg cacggatcgc ggcgtagtcg
      481 gcccggccac gatattccca cagtggtacc gcgatgtcat agagccagta gccgaatccg
      541 cagtcgtcga agtcgatcag ccgcaccgca tcaccgtcga acagagcgtt ctccagatgc
      601 aggtcggcat ggatcaggcc gaaggcgtcc ggcttcgatc caagacccgc catgacagta
      661 cgcatccggc gcgcgacctc atcgaattgc gcgcgcagcg gcaccggcag caggtcccag
      721 cacccggctg cggatacgcc gccgtactcc atggtgttgc cgaagaaggt ctcccagtcc
      781 caccgcatgc gtacgaaccc ggatggcgga gcccacaccg aagcgtgatc gtgcaaacgg
      841 gccaggatgc cacccaatcg ccggaagtgc acggaccgcg gattggccgc ctgcattcgt
      901 ccgttctgcc agccgaggac cgagcagacc cggccgtcgg cgacggtggt ccactgtccg
      961 ctgcaggccc ggatcggcgt cggcacgctc agatccgtgt ctgcctgcaa tgcggccagc
     1021 caggccagtt ccgatccaac cgcgacccgc gaatcgactg cacggccgtg ccggttcggc
     1081 cggtggacgc gcatcaggaa tcgggcatcg cgggagtcga cccgaaacgt ggtgttctcg
     1141 ccgtgcgcga tgaacgacag gcgtggctcg tcgaccgcgt agtccgcaag cgcccgcacg
     1201 gctgtgcggc ggatgcgtgc gacctgtgcg cgcttcgaca ccagttcggg catccgcgca
     1261 acctatcgcc gccgcctcgg tcgccgccag gaattgtccg gcggcccggg cttaggttcg
     1321 gttgatgacc tcaactggca cgctctggcg ggagaccgtg ttgagctgtg ccgatcattg
     1381 aggccgatgt cgacgcgcgc cggtgtgctg tccgttcttt tggtgggtcg cggtgtgacc
     1441 gtgaacctgt cggtctacgt cgacgtgttc gggctcgggt cttgtgtcgt ttcgtcggcg
     1501 cggaagcgag cgatggccaa cagatgcaca accagcagga acggcaccgc gaacgtcggg
     1561 atcaggctta gcggcagttc cgccatgggc ggggtgatga tggttgcgtc cgcggcggtc
     1621 cgctgggcac cgaggtcatg gatcgcgccg agcgcgacgg ccatgatgaa gtcggcgatg
     1681 ccgaacagcg tccacccgag gaacaccggc caggcagcgc gggtccggcg gaccacgtac
     1741 ctggcgacga agggtgcggt cagcgccacg gctaggtcgc ccaggcccgc ggggaacgcg
     1801 aagccgcccg gcagcaggtc ggcggcgtac aggacgagga aggcgaaccc ggccgcgcgc
     1861 aacgcctgta ggttgatcag caacggcagg tcgagcgtgg cggcgaacca gcgtagccgc
     1921 cgcgacgtca gcaacccgac ggccacggcg agcaccggga tcccggcggc cagggcgatg
     1981 cgcagtggcg gttcggtcgc cggacggtcg accaggccta ccgccgcggc gatgtaggcc
     2041 aacgcgaacc acacgacccc gaccaggacc agaacgcggg tgctgctgcg ggtacccgcg
     2101 aaggcagctg acatgactga taggtctcca ccgatgcccg gtggcaaacc cgagagtctg
     2161 tcgggtgaag ggggcccggc ccaggggcga gggtacgcga cgttcagcag cgtgacgacg
     2221 gagatcgggt gcaacaatgc cagctcgggg tggccggcgt cgcccagtgc ccgcaggtag
     2281 gcccaggttc aggcgcaacc gttgtcggca cgttcgctgg actcagcgag cagcgggaag
     2341 ccgtgtacac gcgccgccgc cgttcgacct cgccggtctt cgtacggccg cagctgacaa
     2401 gagcgcgcta gacgacctgc tcctcgacat cgcgtgccgc cgtcgaagcc cgcgaacgcg
     2461 agctgggcgg cgaggtcctc ggcgaactgc ggcgacgggt ccagatcgcc gtcattgacg
     2521 acaactgggc ggcgcaccct gcaaacggtg caacaggtcg actccgacac caccgaggcg
     2581 agccgggcgt tcaccacgat gcgatctgcc gcaccccgca cccaccaaca acatcaccag
     2641 cggggcgagg ccgagcgaag cgggtcccgc ccgaatccct gaccccgcgg ccgaggtcct
     2701 gccgatgaca gtgtgggtga cacgctgtag gagtgggtga cgacttcaga gaaccgtggt
     2761 tccgcgccaa cacggggcag gcagcggcgt tcgagcgaga ggctagaagc gagatcgcg
//
LOCUS       DASRJA010000371         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_553652,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000371 DASRJA010000000
VERSION     DASRJA010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553652"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VFC01_16450"
     CDS             complement(<1..410)
                     /locus_tag="VFC01_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZN81258.1"
                     /translation="MRIARLLGLVLAVLTVGVLLAPTAIGQPPFRLPSYVNDGANALS
                     GSQFSDVQQAVDKLYNDRRIRLWVVYVDTFSGQDAVSWTEATMRASDINANEDAVLAV
                     ATGDRAYALHVPSSVSTSTDDLRRNKVEPALRQG"
     gene            409..1707
                     /locus_tag="VFC01_16455"
     CDS             409..1707
                     /locus_tag="VFC01_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HZN81259.1"
                     /translation="MAHNVVLADCASVAVIGDPYGEFDRQRLVVEPPKAAALPGTNTE
                     HRTDFARDRARVLHGAALRRLADKTQVVGPRQSETPRTRLTHSLEVAQIGRGMAIGLG
                     CDPDLVDMAGLAHDIGHPPYGHNGERALDELAAEHGGFEGNAQNFRILTRLEPKVLDP
                     QDRSAGLNLTRAALDAVTKYPWRRGAKKRKFGFYEDDAAAADWVRSGAPQDRPCLEAQ
                     VMDWADDVAYSVHDVEDGVVSGRIDMRVLADDDAAAVLGRLGESHGMGAGLRADDLVA
                     AAERLSALPVVAAVGKYDATLAASVALKRLTSELVGRFASAAIAATREAYGPGPLVRY
                     QAELRVPELVRAEVTVLKILALQFIMSDPRHLDLQARQRERIGRVAQWVYANAPATLD
                     PIFVPAFNDADDDGARLRVVVDQIASFTEGRLERLEPGLD"
     gene            1712..>2632
                     /gene="dnaG"
                     /locus_tag="VFC01_16460"
     CDS             1712..>2632
                     /gene="dnaG"
                     /locus_tag="VFC01_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZN81260.1"
                     /translation="MAGRIPDRDIAAIRERIRIEDVVGDYVQLRRAGADSLKGLCPFH
                     DEKSPSFHVRPNHGHFHCFGCGEGGDVYAFVQKIEHVSFVEAVELLADRVGYTVTYTG
                     ASATNVQRDRGSRSRLLAANAAAQEFYAAALQSDEAAPARQYLLDRNFDMQAAAHFGC
                     GFAPSGWDSLTKHLLRKGFEFKELEAAGLSREGRRGPMDRFHRRLLWPIRASGSEVIG
                     FGARRIFDDDTMEAKYINTPETVLYKKSSVLFGLDLAKRDIAKSHQAVVVEGYTDVMS
                     MHLAGVTTAVASCGTAFGDEHLAMLRRLMMD"
BASE COUNT          419 a          876 c          916 g          421 t
ORIGIN      
        1 tccccttggc gcagcgcggg ttcgaccttg ttgcggcgca aatcgtcggt gctcgtgctg
       61 acgctggatg gtacgtgaag cgcgtacgcg cggtcgccag tcgccacggc aagaacggca
      121 tcctcgttgg cgttgatgtc gctggcgcgc atggtggctt cggtccagct caccgcgtcc
      181 tgtccggaga aggtgtcgac gtagacgacc cagagccgga tgcgccggtc gttgtacagc
      241 ttgtcgacgg cctgttgcac gtcgctgaac tgcgacccgc tgagcgcatt cgcgccgtcg
      301 ttgacataac tgggcagccg gaacggcggc tgaccgatgg cggtaggggc cagcaggacg
      361 ccgactgtca ggaccgccag cacgaggccg agcaaacggg cgatgcgcat ggcccacaat
      421 gtagtactgg cagactgtgc gtcggtggct gtgattggag atccctacgg cgagttcgac
      481 cgccaacggc tggtcgtcga accgccgaag gctgccgccc tgccaggcac caacaccgaa
      541 catcgcaccg atttcgcccg tgaccgggcc cgcgtcctgc acggcgcggc cttacgcaga
      601 ctggcggaca agactcaggt ggtcggaccg cgccagagcg agacgccacg cacccggctc
      661 acccactcat tggaggtggc gcagatcggc cgcggcatgg ccatcgggct ggggtgcgat
      721 cccgacctgg tcgacatggc cggcctggcc cacgatatcg gccacccgcc gtacggtcac
      781 aacggcgaac gggccctcga cgagctggcc gccgagcacg gcggcttcga gggcaacgcg
      841 cagaatttcc gcatcctcac ccgtctcgaa cccaaagtcc ttgaccccca agaccgcagc
      901 gccgggctga acttgacgcg ggccgcgctc gatgcggtga cgaagtaccc gtggcggcgc
      961 ggcgccaaga agcgcaagtt cggcttctat gaggacgacg ccgccgctgc cgattgggtg
     1021 cgctccggcg ccccacagga ccggccgtgc ctggaggccc aggtgatgga ctgggccgac
     1081 gatgtcgcct actcggtgca tgacgtcgag gacggcgtcg tctccggccg catcgacatg
     1141 cgggtgctgg ccgacgacga cgcggccgcg gtgcttggac gcctcggcga gtctcacggg
     1201 atgggcgcag gcctgcgggc cgacgacctg gtggccgccg cggagcggct gtcggcgttg
     1261 ccggtcgtcg cggccgtcgg caagtacgac gccacgctgg ccgcatcggt cgcgctgaag
     1321 cggttgacca gcgagctggt gggccggttc gcgtcggccg ccatcgccgc cacccgcgag
     1381 gcctacggcc cgggtccgct ggttcgctat caggccgagc tgcgcgtgcc cgagctggtg
     1441 cgagccgagg tcacggtgct gaagatcctg gcgctgcagt tcatcatgtc ggacccgcgc
     1501 cacctcgacc tgcaggcccg ccagcgcgag cggattggtc gcgtcgcgca gtgggtgtat
     1561 gcgaatgcac cggcgacgct cgacccgatc tttgtgcccg ccttcaacga cgccgacgac
     1621 gacggtgcgc ggctgcgggt ggtcgtcgac cagatcgcgt cgttcacaga agggcgattg
     1681 gagcgcctcg agccgggcct agactaggcc ggtggccggc cggattcctg atcgcgatat
     1741 cgcggccatt cgtgaacgca tccggatcga ggacgtcgtc ggcgactacg tgcagctgcg
     1801 gcgcgcgggt gcggattcgc tgaaggggct ctgcccgttc catgatgaga agtcgccgtc
     1861 gtttcacgtc cggcccaacc acggccattt ccactgcttc ggctgcggcg agggcggcga
     1921 cgtctacgcg ttcgtccaga agatcgagca tgtcagcttc gtcgaggccg tcgagttgct
     1981 cgccgaccgg gtcggctaca ccgtcaccta caccggcgcg tcagcgacga acgtgcagcg
     2041 cgaccgaggc agccgcagca ggctgctggc cgccaacgcc gccgcgcagg agttctacgc
     2101 cgcggccctg cagtctgacg aggccgcacc cgcgcggcag tacctgctcg accgtaactt
     2161 cgacatgcag gccgccgcgc atttcggctg cggattcgcg ccatcgggct gggattcgct
     2221 gacaaagcat ctgctgcgca agggatttga gttcaaggag ctcgaggccg ccggcttgtc
     2281 gcgggagggt cgacgcgggc cgatggaccg cttccaccgc cggctgttgt ggccgatcag
     2341 ggccagcggc agcgaggtca tcggcttcgg ggcgcgacgc atcttcgacg acgacacgat
     2401 ggaagcgaag tacattaaca ccccggagac ggtgctctac aagaagtcat cggtgttgtt
     2461 cggcctcgat ctggccaagc gcgacatcgc caagtcacat caggcggtgg tggtcgaggg
     2521 ctacaccgat gtgatgtcca tgcacctcgc cggagtgacc actgccgtcg cctcctgcgg
     2581 caccgcgttc ggcgatgagc acctggcgat gctgcgccga ctcatgatgg ac
//
LOCUS       DASRJA010000372         6894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3137094,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000372 DASRJA010000000
VERSION     DASRJA010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3137094"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFC01_16465"
     CDS             complement(<1..452)
                     /locus_tag="VFC01_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN81261.1"
                     /translation="MQIAGSSAIVVGGAGGLGEATVRRLHGAGAKVVVADLADDKGKE
                     LESELGVRYVRTDATSEESVNAAIAEASALAPLRISVDTHGGPASGGRLIGKDGSPLD
                     LDGFKKTIEFYLTAVFNVMRLSAAEIAKTEPLEEGGRGVIVNTASIAG"
     gene            524..1663
                     /locus_tag="VFC01_16470"
     CDS             524..1663
                     /locus_tag="VFC01_16470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZN81262.1"
                     /translation="MPDAVIVSALRTPIGTAMKGTLRDTDAYHLAEHVVGATVAELRS
                     AQIDDVILGEGLYGGGVVARHAAITAGLASVPGLAVNRHCAAGQAAVQSAAASIRAGM
                     DQLILAGGVNSASTSPRFKRRVGDEMVDWFPPTHPDRPDAPNMDMSITVGWNAAVKAG
                     VSREEMDEWALGSHRKAIQAIDEGRFKHEIVPIETPHGLFEVDEHPRRDTSLEKLAAL
                     KPLHPEIEGFSITAGNACGGNDGAAILGVASDRLGLPALATIRSWASVGVDPASTGLA
                     PVEAIPKALARARLSLADVDLFEINEAFASMCVATIKLLDIDPAKVNPSGSGCSLGHP
                     VAATGARMLVTLVHELRRRGGGIGVAAMCAGGGMGSATVIEVSAP"
     gene            1674..2567
                     /gene="meaB"
                     /locus_tag="VFC01_16475"
     CDS             1674..2567
                     /gene="meaB"
                     /locus_tag="VFC01_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HZN81263.1"
                     /translation="MTIDELIEAARKGSTRAAGRLLSLVESPRRTEVLDALGPTTPPR
                     IVGVTGPPGAGKSTTVGALVGAYRGRDLRVAVLAVDPSSPYSGGALLGDRIRMAAHIN
                     DPDVLIRSVATRGHLGGLAAAVPAAIRLLAALSYDLIVLETVGVGQSEIEIAAVADPT
                     IVILNPGAGDAVQAAKAGLLEVADIVVVNKADREGADQTVRDLRAETMAPILKLIAAQ
                     GDGIAELIDAIEEHHRADSPERRTARARAQILSLVQTLLRNHPDLDRLATEVAEGTHD
                     PYAAAEQLFAVPVETNPKGQA"
     gene            2564..3409
                     /locus_tag="VFC01_16480"
     CDS             2564..3409
                     /locus_tag="VFC01_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN81264.1"
                     /translation="MTTAEVLAIIGVGGMGVAVARRMGAGRVIVLGDVNTAQVDSVAG
                     QLADDGHHVVTGHVDVTSRESVTEFARLAASCGRVSSVVHTAGLSPQQAAAEVVLAVD
                     LLGVALTLEVFGDIIEPGGAGVVISSMAGHFLPPIPQDVERQLATVPVDELLGLPACG
                     PAAITSSEAAYPFAKRANQLRVAAAASQWGRRGARINSISPGVIATAMGRRELAGPSG
                     DSMRAMVDGSGVRRLGTPDDIAAATEFLLSPAASFITGIDLLVDGGVVAAVRTGTIDL
                     PAAAG"
     gene            complement(3389..3991)
                     /locus_tag="VFC01_16485"
     CDS             complement(3389..3991)
                     /locus_tag="VFC01_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN81265.1"
                     /translation="MTSTQEDRRFSEITRTAAQTRVLDAALKLISERGVSGTSLQMIA
                     DEMGVTKAAVYRQFKTKEEIVIAITEREMSRLEDAVEAAEAQGHPLRAREVLLDRMIE
                     QAIDRRGAFSVPLFDPVIIRLQAEYEPFQRFIERLYAALLGTEAGVEARLHAAMLSSA
                     ISVAVMHPLLADVDGATLRTQLKEMTRRMLDLPASTGRGR"
     gene            4089..4796
                     /locus_tag="VFC01_16490"
     CDS             4089..4796
                     /locus_tag="VFC01_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81266.1"
                     /translation="MRRTDGELILFWTLPAIAIIWVSAFFLFPGFLHPMSPTMSTDEV
                     AAFYRDETARIRYSMILFNWFGVGLIPMVILLAMQVRRMAHRTPIVSYSLIACAGGPP
                     TLFLIANMFWLLGSFRPERSPELTQLLNDLAWITFTVLVPYLIAQCLLLALAIYWDRQ
                     EQPVFKMWVAHFNILVAVALAPAAFTALTFSGPLAWDGLLSFWVKNIAIAAWIVVMAV
                     VLGQAILRRRVEEPVAA"
     gene            4793..5599
                     /locus_tag="VFC01_16495"
     CDS             4793..5599
                     /locus_tag="VFC01_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81267.1"
                     /translation="MSTRAGSPVGRFLWNFRNHPKRELWFAWWVMVIFYQLYGVLFFL
                     VTRVQPPASPAWDTPMVVQWFNDRHFGLLAGFGVVFLISGLCAPMNALLAYSMRRMSV
                     SRVFGYSYLIMYSLSAIPGMFVMAIAMTVGALRPDRDSELIHWLFDFAFLSFSGTMGV
                     FMIGSLVWLVAILIDKNGVFPKWFGYLGLCNALTEVVVAPAWIFQRGVFAWNGAIAWW
                     INMVVFVTYTVCFIVLLRKMIEREDFGTGPLPDLPPKAERSQPDSVGSMQ"
     gene            5596..6198
                     /locus_tag="VFC01_16500"
     CDS             5596..6198
                     /locus_tag="VFC01_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZN81268.1"
                     /translation="MTNLQEDSSTEQVDRKAAARFVPGQPDMWVFVLFESLLFTGYFS
                     VYLFGRIQNRDLYLQSQADLDLRFGVFNTIVLLLSSWAIARCVQASREGAYRDALRDA
                     FLTIGFGVAFLVSKVLEWVKEIRLGNTFTSNEFFQYFFFLTSIHLLHLLIGFIVLGVA
                     VYQLWSPARRSQQLVETCATYWHTVDFLWALIFALLYVVR"
     gene            6202..6498
                     /locus_tag="VFC01_16505"
     CDS             6202..6498
                     /locus_tag="VFC01_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HZN81269.1"
                     /translation="MSGTFNKRLAIVWLILSAMTLVYVWLDVAVDQNGTLKASTVVTV
                     SAIIIALVKVRIIFREFMEVRHAPALLRRLTDAWVVLMAVCLLGSYFIGSALAS"
     gene            complement(6515..>6894)
                     /locus_tag="VFC01_16510"
     CDS             complement(6515..>6894)
                     /locus_tag="VFC01_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81270.1"
                     /translation="RGIVHVRELLEYADGRAESGMESEARLVMIDYGLPLPELQYEIP
                     GRDGEVWRVDFAWPEHRVAAEYESIDWHVGRAEMLRDKKRFAGIQETAWTVIPIVVDD
                     VRRHPQRFAQRVDYHLSRAQIAS"
BASE COUNT         1132 a         2194 c         2181 g         1387 t
ORIGIN      
        1 tagccggcga tcgacgcggt gttgacgatg acgccgcggc cgccctcttc gaggggctcg
       61 gtcttggcga tctcggccgc tgacagccgc atcacgttga agacggcggt caggtagaac
      121 tcgatcgtct tcttgaagcc gtcgaggtcg agcggcgacc cgtccttgcc gatcagccgc
      181 ccgccgctgg ctggaccgcc atgcgtgtcg acggagatcc gcaggggagc cagcgccgac
      241 gcctccgcaa tggccgcgtt gaccgactct tccgaggtgg cgtcggtgcg gacatatcgg
      301 acaccaagct cgctctcgag ctccttgccc ttgtcatcgg cgaggtcggc gacgaccacc
      361 ttggctcccg cgccatgcag gcggcggacg gttgcctcac ccaggccacc tgcgccacca
      421 acgacgatcg ccgagctacc tgcgatttgc atacggttcc ccttcttgca actggcactc
      481 tcgaactgag agaatagcat tctcatgcga aacggagaca cccatgcctg acgccgtcat
      541 tgtgtccgcc ctgcgtacgc ccatcggcac cgcgatgaag ggcacgcttc gggacaccga
      601 cgcctaccac ctggcagagc atgtggtcgg cgcgacggta gccgaactcc gtagtgcgca
      661 gatcgacgac gtgatcctgg gtgagggcct ctatggcggc ggggtcgtcg cgagacacgc
      721 ggcgatcacc gcgggtctcg cctcggtgcc gggcctcgcg gtcaaccggc actgcgccgc
      781 cggccaggcc gccgtgcaga gcgcagcggc cagcattcgg gccggcatgg atcagctcat
      841 cctcgccggc ggcgtcaact ccgcgtcgac gtcgccacgc tttaagcgac gggtcggcga
      901 cgagatggtc gactggttcc cgcccacaca tccggacagg ccggacgcgc cgaacatgga
      961 tatgtcgatc actgtcggct ggaacgccgc ggtcaaggcc ggcgtgtccc gcgaggagat
     1021 ggacgagtgg gcactcggct cgcatcgcaa ggccatccag gcgatcgacg agggccgctt
     1081 caaacacgag atcgtgccga tcgagacgcc tcacggcttg ttcgaggtcg acgaacatcc
     1141 gcgccgcgac accagcttgg agaagctggc cgcgctcaag ccccttcatc ctgagatcga
     1201 agggttctcg attaccgcgg gcaacgcgtg cggcggcaac gatggcgcgg cgatacttgg
     1261 tgttgcgagc gaccgcctcg ggctgcccgc gctggcgact atccggtcgt gggcgtcggt
     1321 cggtgtcgat ccggcgtcga cgggtctggc gccggttgag gcgattccga aagctcttgc
     1381 gcgtgcccgc ctttcgctcg ctgacgtcga cttgttcgag atcaacgagg cgttcgcgtc
     1441 gatgtgcgtg gcgaccatca agctgctcga catcgacccg gccaaggtca atccgagcgg
     1501 tagcggctgc tcgctcggtc atccggtcgc agccacgggc gcacggatgc tcgtcacgct
     1561 tgtgcacgaa ctgcgtaggc gtggcggcgg aatcggtgtc gcggcgatgt gcgcgggtgg
     1621 cggaatggga tcggccacgg ttatcgaggt gtccgctccc tagactgaca gagatgacca
     1681 tcgacgagct catcgaggcg gcgcgcaagg gatccacccg agcggccggc cggctgttga
     1741 gccttgtcga aagtccccgc cgcaccgagg ttctggatgc tctcgggcca accacaccgc
     1801 cgcgcatcgt cggcgtgacg ggtccgccag gtgctggaaa gtcgacgacg gtcggcgcgt
     1861 tggtcggtgc ctatcgcgga cgcgacctgc gggtcgcggt gctcgctgtc gacccatcgt
     1921 cgccgtacag cgggggagca ctgctcggag accgtatccg aatggccgcg catatcaacg
     1981 accccgacgt gttgatccga tcggtggcca cccgtggaca ccttggcggc ctggcggcag
     2041 cggtcccagc cgcgatccgg ctgctcgcgg ccttgtcgta cgacctgatc gtgttggaaa
     2101 ccgttggcgt tggtcagtcg gagatcgaga tcgctgcggt tgccgatccg acgatcgtca
     2161 ttttgaatcc cggtgccggc gacgcagttc aggccgcgaa ggcgggacta ctcgaggtgg
     2221 ccgacattgt cgtcgtgaac aaggccgacc gcgagggcgc cgaccaaacc gtgcgcgacc
     2281 tgcgtgccga gaccatggcg ccgatcctca aactcattgc cgcacaaggt gacggcatcg
     2341 ccgagctcat cgacgcaatc gaggaacatc accgcgcgga cagtcccgaa cggcgaacgg
     2401 cgcgggcgcg agcgcaaatt ctatctctgg tgcagacgct gctacgcaat catcccgacc
     2461 tggatcgact ggcgacggag gtagccgagg gcacccatga tccctacgcc gctgctgaac
     2521 agctcttcgc agttcctgtt gagacaaacc cgaaaggtca agcatgacaa cagctgaagt
     2581 tctcgcgatc atcggcgtcg gcggcatggg cgtcgccgtg gctcgccgga tgggagcggg
     2641 acgtgtgatc gtgctcggcg atgtgaacac tgcacaagtc gacagcgtgg ccggccagtt
     2701 ggccgacgac ggtcatcacg tcgtgacggg ccacgtcgat gtcacatcgc gggagtcggt
     2761 caccgagttc gcgcgcttgg ccgcctcatg cggtcgcgtg tcctcggttg tccacaccgc
     2821 gggtctttcg ccccaacaag ccgcagcgga agtcgtactc gcagtggatc ttctgggcgt
     2881 cgcgttgact ctggaagtgt tcggcgacat catcgagccc ggcggagccg gcgtggtgat
     2941 ttcgagcatg gccggtcact tcctgccgcc gataccgcag gatgtcgagc gccagttggc
     3001 aaccgtaccc gtcgacgagc tgctcggttt gcccgcttgc ggaccagcgg cgatcacgag
     3061 cagtgaagcg gcttatccat ttgccaagcg tgccaaccaa cttcgggtgg cggccgccgc
     3121 gtcgcaatgg ggccgtcggg gcgcccgaat caattccatc agcccgggtg tcatcgcgac
     3181 tgcgatggga cggcgtgaac tcgccggacc gtccggcgac agcatgcggg ccatggtcga
     3241 cggctctggc gttcgacggc ttggcacgcc cgatgacatc gccgccgcta ccgagttcct
     3301 gctcagtccc gcggcaagtt tcatcacggg tatcgatctt ctggtcgacg gtggcgtggt
     3361 cgcggccgtt cgaaccggaa caatcgactt accggccgcg gccggttgac gcgggcaggt
     3421 cgagcatccg ccgagtcatc tctttcaact gagtccgaag tgtcgcgccg tcgacgtcgg
     3481 cgagtagcgg atgcatgacg gccacgctga tcgcgctcga cagcatcgcc gcgtgcaggc
     3541 gcgcctcgac tcccgcctcc gtgcccagca atgccgcata gagacgttcg ataaaccgct
     3601 ggaacggttc gtattccgcc tgcaaccgaa tgatgacggg atcgaacagc ggcacgctga
     3661 acgcgccgcg gcggtcgatg gcctgctcga tcatccggtc aaggaggacc tcccgcgctc
     3721 gcaacgggtg cccctgcgcc tcggccgcct cgaccgcatc ctcgagtctg ctcatctcgc
     3781 gctcggtaat cgcgatgaca atctcttcct tggtcttgaa ctggcggtac acggccgcct
     3841 tcgtcacgcc catctcgtcg gcgatcatct gcagcgacgt cccgctgacg ccgcgttcgg
     3901 agatcaactt caaggctgcg tccagcacgc gggtctgagc agcggtacgc gtgatttcgc
     3961 tgaacctgcg gtcttcctgt gtggacgtca cagcgacgag ggtaaccgac tattgaccgt
     4021 ccagttgccg atcggctacc gtccaagttg ccgatcggca accacgctac gcatgaagag
     4081 gttcgacgat gagacgcacc gatggggagt tgatcctttt ctggaccttg ccggcaatcg
     4141 cgatcatttg ggtgtccgcg ttctttctgt ttcccggttt ccttcacccc atgtcgccaa
     4201 cgatgtcgac ggacgaggtc gcagcgttct atcgcgacga gaccgcgcgg atccgctaca
     4261 gcatgatcct gttcaactgg ttcggcgtgg gtctgatccc catggtgatc ctcttggcga
     4321 tgcaggtacg ccggatggct catcggacgc cgatcgtgtc ctacagcctg atcgcctgcg
     4381 ccggcggccc gcccacgctg tttctcatcg ccaacatgtt ctggctcctc ggctccttcc
     4441 gaccagaacg ttccccggag ttgacccagc tgctcaacga tctcgcgtgg ataaccttca
     4501 ccgtcctggt tccgtacctg atcgcccaat gcctactgct cgcgctggcg atctactggg
     4561 atcgccagga gcaacccgtt ttcaagatgt gggtggcaca cttcaatatt cttgtcgcgg
     4621 tcgccttggc gcctgccgct ttcaccgcgc tgacgttcag cgggccgctg gcgtgggacg
     4681 ggctgctgtc cttctgggtc aagaacatcg cgatcgccgc ctggatcgtc gtgatggcag
     4741 tcgttctcgg ccaagccatc ctgcgccggc gcgtcgagga gccggtcgct gcatgagtac
     4801 acgagcagga tcaccggtcg gccggttcct ctggaatttc cgtaaccatc ccaagcgcga
     4861 gttgtggttc gcgtggtggg tgatggtgat cttctaccag ctgtacggtg tgctgttctt
     4921 cttggtcacg cgtgtccagc ctccggcgag cccggcctgg gataccccaa tggtcgtgca
     4981 gtggttcaat gaccggcact tcggccttct tgccggattc ggcgtcgtgt tcctcatctc
     5041 aggcctgtgc gcgccgatga atgcccttct cgcatactcg atgcggcgca tgtcggtgag
     5101 tcgcgtcttc ggatactcgt acctcatcat gtattcgctg agcgccatcc ccggcatgtt
     5161 tgtgatggcc atcgcgatga ccgtcggagc cctgcggccg gaccgcgatt ccgaactgat
     5221 ccactggctc tttgacttcg cgttcctgtc gttcagcggc accatggggg tattcatgat
     5281 cggctccctg gtctggctgg tcgccatatt gatcgacaag aatggcgtgt tcccgaagtg
     5341 gttcggttat ctgggtcttt gcaatgcgct gaccgaggtg gtcgtcgcgc ctgcgtggat
     5401 ctttcaacgc ggcgtgttcg cctggaatgg tgcgatcgcg tggtggatca acatggtggt
     5461 attcgtcacc tacaccgtct gcttcatcgt gctgctgcgg aagatgatcg agcgcgagga
     5521 cttcggcacc gggccgttac ctgatctacc gcccaaagcg gaacgtagcc agccggattc
     5581 ggtggggtca atgcaatgac gaacctgcag gaagactcct caacggaaca ggttgaccgc
     5641 aaggccgccg cccggttcgt gccggggcag ccggacatgt gggtgttcgt tcttttcgaa
     5701 tcgctgctct tcacggggta tttctcggtc tacctcttcg gccgtatcca aaaccgggac
     5761 ctttacctac agtcccaagc cgatctggac ctccgcttcg gggtcttcaa caccattgtc
     5821 ttactgctga gttcgtgggc gatcgcccgg tgtgtccaag cgtctcggga gggtgcgtac
     5881 cgggacgcac tgagggacgc gttcctgacg atcggcttcg gcgtagcgtt tctggtgtcc
     5941 aaggtgctcg agtgggtcaa ggagattcga ctcgggaaca cgttcaccag caacgagttc
     6001 ttccaatact tcttctttct gacgtcgatc caccttctgc atctgctaat cggcttcatc
     6061 gtgctgggtg tcgccgtgta ccagttgtgg agcccggccc ggcgatctca gcagctggtg
     6121 gaaacgtgcg cgacctattg gcacacggtc gatttcctgt gggccctgat ctttgcattg
     6181 ctctacgtcg tgaggtgatc cgtgagcgga acattcaaca agaggctggc gatcgtctgg
     6241 ctgatcctgt cggcgatgac gctggtttac gtttggctag atgtagcggt cgaccagaac
     6301 gggacgctga aggccagcac agtcgtcacc gtgagcgcga tcatcattgc tctcgtgaag
     6361 gtgcgcatta tcttccgcga gttcatggaa gtgcgacatg cccccgcatt gttgcgccgc
     6421 ctcaccgatg catgggttgt gctgatggcg gtgtgcctgt tggggagcta cttcattggc
     6481 tcggcgttag cgagctaaac ctgcgcgccg gggttcaact ggcgatctgc gcccgtgaca
     6541 ggtggtagtc gacgcgctgc gcgaagcgct gcggatgccg ccgcacgtca tcgacgacta
     6601 ttggaatgac agtccacgcc gtctcctgaa taccagcgaa tcgcttcttg tcgcgaagca
     6661 tctcggccct gcctacgtgc cagtcgatgc tttcgtactc tgcggctacc cggtgctcag
     6721 gccaggcaaa gtcgacacgc catacctcac cgtcgcgccc tgggatttcg tactgcagct
     6781 ctggtaaagg caggccatag tcgatcatga ccagtcgggc ctcgctctcc atacccgact
     6841 ccgcgcggcc gtcggcgtac tccaacagct ctcgcacgtg aacgatcccg cggc
//
LOCUS       DASRJA010000373         4493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1030680,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000373 DASRJA010000000
VERSION     DASRJA010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030680"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VFC01_16515"
     CDS             <1..1341
                     /locus_tag="VFC01_16515"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HZN81271.1"
                     /translation="QVRAGLNYRFGGGGTNTNGGSIGLPTPATDIVNFHGQTTFVWQA
                     YPAIRSPYTGPFSLPGSGLGRETFDTTLYAGIRLWQGAELWISPEIDQGFGFADTHGA
                     AGFPSGESYKLGSSYPYVRMHRGFLRQTINLGGDVEKVDADISQFAGARTANRLVLTI
                     GKFAIVDIFDTNRYANSPKTDFLNWSLINAGTFDYAGDGWGYTYGAAAEWYQGSWTLR
                     AGVFDLSATPAGGNSPNGGVLDATFQQYAVVAEIEKRYELWGQPGKFKVTGFLNRGRA
                     AKFQDAIALAQITGEPADVALVRNYTGRPGVSLNLEQQISDTVGVFARAGWADGNIEP
                     WDFTDIDRTVAGGVAINGKNWGRPDDTVGIAGVINDISGIHRAYFNAGGLGILIGDGQ
                     LPNPGLEKIFETYYSYALSPDMRLSFDYQFIANPAYNTDRGPVNTFAARFHAQF"
     gene            1624..3825
                     /locus_tag="VFC01_16520"
     CDS             1624..3825
                     /locus_tag="VFC01_16520"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81272.1"
                     /translation="MAKKIEGMPWDDGGWFMGWGVDWWRGLKTVGLIGTDDAEEIYGY
                     GGNDVLHGNGGNDKLDGGTGHDFMNGGMGNDTYYVDDAGDFAVDDADVYDAQGNPANA
                     GYDRVYASVSHTLGFGIEELRLVEGSSAANGTGNELNNTIHGNSNDNELRGGGGRDTL
                     YGNGDNDTLRGEAGDDYLYGGSHGDSLFGGANNDLLDGGTGGDSMRGEAGNDRYYVDN
                     ELDQVFENANEGWDTVYSSRYSFTLAYNFEELRLLDGAVFGTGNARDNEIYGNDAGNT
                     LDGSFGNDDMVGNGGNDFYWVDSAGDRVFESANDGRDQVFVKNVVDYRLSANVENLSL
                     FEGAVNATGNELANGIAGNDNGNSLDGGFGNDSLYGLGGIDTLSFMSWDLGTQGNSES
                     IRIDLSTGLAQRKQTIPGVGTSVLETDTFSEFENVRGSNRHETIVGTGGDNTLEGRGG
                     DDVLQGNYGNDGLSGGTGLDTASYQDNLGRVFVNLGWNGATGNALEYFTMPSGQQAGL
                     EVVLSTDALVSIENVRGSAFADYILGNQEDNIIDGRGSADQMIGLTGNDTYLVDNAND
                     AVVEGTGLGTLDVVRTSVSYALAAGTEVEVLETTNQAGTASINLTGNEFGNTINGNNG
                     ANVIDGGHGNDTLFGGGNADIFVFDHIVNGSQQDVVLDFQAGIDHIDLSDTLVDNFAD
                     LLDYTSLDNPGNKYMEQVGNDVLIHTSVSAGTSILLENVQLSSLTSADFLF"
BASE COUNT          918 a         1434 c         1384 g          757 t
ORIGIN      
        1 caggtgcgcg cagggctgaa ctatcgcttc ggcggcggcg ggacgaacac aaacggcggc
       61 tcgatcgggc tgcccacgcc ggcgacagac atcgtgaact ttcatggtca aaccacgttc
      121 gtgtggcaag cctacccggc gatccgatcg ccctataccg gccccttcag cctgcccggc
      181 agcgggctcg gccgcgaaac gttcgatacg acgctctatg ccggcatacg gctgtggcag
      241 ggcgccgagc tgtggatcag cccggagatc gatcaaggtt tcggctttgc ggacacgcac
      301 ggcgccgccg gatttccgag cggcgagtcc tacaagctcg ggtcatccta cccttacgtg
      361 cgcatgcacc gcggattcct ccggcagacc atcaatctcg gcggcgacgt cgagaaggtc
      421 gacgccgata tcagccagtt cgccggggcg cgcacggcga accgcctcgt gctgacgatc
      481 ggaaagttcg ccatcgtcga catcttcgat accaacagat atgccaacag tcccaagacc
      541 gactttctga actggtcgct gatcaacgcc ggcacattcg actatgccgg cgatggctgg
      601 ggctacacct atggtgcagc cgccgagtgg taccagggct cgtggacctt gcgcgccggc
      661 gtgtttgacc tgtcggcgac tcccgccggc ggcaacagtc ccaacggcgg cgtcctcgac
      721 gcgacctttc agcagtacgc ggtggttgcg gaaatcgaga agcgctacga attgtggggc
      781 cagcccggca agttcaaggt caccggcttc ctcaaccgcg gccgggcggc aaaatttcag
      841 gacgctatcg cgctcgccca gatcaccggt gagcccgccg acgttgcgtt ggtgagaaac
      901 tataccggcc gtcccggtgt cagcctgaac ctggaacagc aaatctcgga caccgtcgga
      961 gtattcgccc gcgccggctg ggccgacggc aacatcgagc cctgggactt caccgacatc
     1021 gaccggacgg tcgcgggcgg cgtcgccatc aacggcaaga attggggccg tccggacgat
     1081 accgtcggaa tcgccggtgt catcaacgac atcagcggca ttcaccgggc gtatttcaac
     1141 gccggcggcc tcggcatcct catcggcgat ggacaactgc cgaatcccgg gctcgagaaa
     1201 atcttcgaga cttattacag ctacgcgctt tcacccgaca tgcgcctcag cttcgactat
     1261 cagttcatcg ccaaccccgc ctacaacacc gaccgtggcc cggtaaacac gttcgccgca
     1321 cgcttccatg cccagttcta gagcgcgcgc atctcgcggc cgcgattcaa gctgaccgcc
     1381 acactagggc cggcatcgta ttcctgcaga aaagccttgt cccgcggcac gattctagtt
     1441 gtcggaccgc tgcgcccgga aggttcaccg aaagcgcatt tgtgtgccgg ccatcacatc
     1501 ccgtgatcgg attcaccggt tatctgcgga ccattggcac ggttggccgt aggacgcagt
     1561 cggcggacca cacaaccggg cgggtggaaa gtgatcggca tcacacaagg gagggcgacg
     1621 acaatggcca agaagatcga gggaatgcct tgggacgacg gtggctggtt catgggttgg
     1681 ggcgttgatt ggtggcgggg cctcaagacg gtcggattga tcggcaccga cgacgccgag
     1741 gaaatctacg gctatggcgg caacgacgtg ctccacggca atggcggcaa cgacaagctc
     1801 gacggaggga cgggccacga tttcatgaac gggggcatgg gtaacgacac ctattacgta
     1861 gacgatgccg gcgacttcgc ggtcgacgat gccgatgtgt acgacgccca gggcaatccg
     1921 gcgaacgccg gctacgacag ggtgtacgcc tccgtcagcc acacgctcgg cttcggcatc
     1981 gaggagctcc ggctcgtcga aggctcgtcg gcggccaacg gcaccggcaa cgagctcaac
     2041 aacaccatcc acggcaattc caacgacaac gagctgcgcg gcggcggcgg ccgcgacacg
     2101 ctctacggca acggcgacaa cgacacgctg cggggcgagg ccggcgacga ctacctctat
     2161 ggcggatcac acggcgattc gctgtttggt ggcgccaaca acgatctgct cgacggcggc
     2221 accggtggcg actccatgcg gggcgaagcc gggaacgaca ggtattatgt cgataacgag
     2281 ctcgaccagg tgttcgagaa cgccaatgag ggttgggaca cggtctactc ctcgcgctac
     2341 agcttcacgc tcgcgtacaa cttcgaagag ttgaggctgc tggacggagc cgtcttcggc
     2401 actggcaacg ctcgcgacaa cgagatctac ggcaacgacg ccggcaatac gctcgacggg
     2461 agcttcggca acgatgacat ggtgggcaac ggcggcaacg acttctactg ggtcgacagc
     2521 gccggcgaca gggtgttcga gtccgccaac gacggccgcg accaggtctt cgtcaagaac
     2581 gtcgtcgact acaggctgag cgccaatgtc gagaacctct ctttgttcga gggggccgtc
     2641 aacgccaccg gcaacgagct ggccaacggc atcgccggca acgacaacgg caattcgctc
     2701 gatggcggtt tcggcaacga cagcctgtac ggcctcggcg gcatcgatac gctcagcttc
     2761 atgagctggg atttggggac tcaggggaac tcggagagca ttcgcatcga tctgtcgacg
     2821 ggtttggcgc agcgcaagca gaccattcct ggggtcggga catcggtgct ggagaccgac
     2881 acgttctccg agttcgagaa cgtgcgcggc tccaaccggc atgaaaccat cgtcggcacc
     2941 ggcggcgaca atacgctcga gggacgcggc ggcgatgacg tccttcaggg caattacggc
     3001 aacgacggtc tcagtggcgg aaccggcctc gacaccgcga gctaccagga caacctcggt
     3061 cgcgtcttcg tcaacctcgg ctggaatggc gccaccggta atgcattgga atacttcacg
     3121 atgccctccg gccagcaggc gggcctggag gtcgtgctct ccacagacgc gctggtcagc
     3181 atcgaaaatg tccgcggctc ggccttcgcc gattacatcc tcggcaacca ggaggacaac
     3241 atcatcgacg gccgcggcag cgcggaccag atgattggcc tcaccggtaa cgacacctac
     3301 cttgtcgaca atgccaacga tgccgtggtc gagggcaccg gcttgggcac gctcgatgtc
     3361 gtgcggacga gtgtgagtta cgccctcgcg gcgggcaccg aagtcgaggt gctggaaacc
     3421 accaaccagg ccggcaccgc gagcatcaat ctcaccggca acgaattcgg aaataccatc
     3481 aacggcaaca atggggccaa cgtcatcgac ggcggccacg gcaacgacac cttgttcggc
     3541 ggaggaaatg ccgacatctt cgtgttcgat catatcgtca atggcagcca gcaggacgtc
     3601 gtgctggact tccaggcggg tatcgaccac atcgatctca gcgacaccct ggtcgacaac
     3661 ttcgccgacc tgctcgacta caccagcctg gacaatcccg gcaacaagta catggagcag
     3721 gtcggcaacg atgtgctcat ccacacgtcg gtgtccgcgg gcaccagcat cctgctggag
     3781 aacgtgcagc tgtcgagcct gaccagcgcc gacttcttgt tctgagttcc gcatccggcc
     3841 cggtcgccgg ctattgtcgg cggtcggttc tccaatggga acgatccccg ccccttgctt
     3901 gaggatgctg ctcgcgcgtg ccgacaaggt gatcggatca gggagccaat gccggctcgt
     3961 ggcacacctt gagaccttcc gagatgtccg ctcattggtc gctattggag gcgaagcgga
     4021 catcagcgat cagcgatcgc tgagacgaaa tgggtacgcg ccctagtcag cgcgagcttc
     4081 ttccatcggt gcgaaggtcg ctatagccgg acttagcacc agcccttctt ccagtgtccc
     4141 ggcggacaat gccctcggcc ccaccagcca gggacgccct tcttctttac cacgatagtg
     4201 tcactcaagg tgttcgcggc cagacgcatc ccgcccttga acgggacggc ggctcttgcg
     4261 ggtgtgaatg ccgcaaacca tagcgcccca accagtatcc cgcatgtcac aaccactcgc
     4321 atcatgctcc tccttaaatc agggcgagcg cccatcgcct cttccatcag tgacgaacga
     4381 atctcggctt gcggccagcg ggggacgggc cgcctggcaa ggagcaaaca gggtctcgac
     4441 cgactgcggc gatggcggct gacaactttc ggcaggaaag aaatgccaag acg
//
LOCUS       DASRJA010000374         7381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2054954,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000374 DASRJA010000000
VERSION     DASRJA010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054954"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VFC01_16525"
     CDS             complement(<1..902)
                     /locus_tag="VFC01_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZN81273.1"
                     /translation="MSSLRRGDRGGAVAEIRAALSALAMIDNPDEDLNTGRQIAVEVF
                     DDELDHAVRAFQQHRGLLVDGIVGEATFRALKEASYRLGARTLNHQFGAPMYGDDVAT
                     LQARLQDLGFYTGLVDGHFGLQTHNALMSYQREYGLYPDGICGPETLRSLYFLGSRVT
                     GGSPHAIREEELVRRSGPRLSGKRIIIDPGRGGNDHGLIMNGPAGPISEADILWDLAT
                     RLEGRMTAIGMETFLSRPANRSPSDAERADTANTVGADLMISLRCATLASPAANGVAS
                     FHFGNSHGSVSTIGRNLADFIQREV"
     gene            complement(1052..1387)
                     /gene="trxA"
                     /locus_tag="VFC01_16530"
     CDS             complement(1052..1387)
                     /gene="trxA"
                     /locus_tag="VFC01_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZN81274.1"
                     /translation="MTEKTSATVAVTDDSFSEDVLASSTPVLVDFWATWCGPCKMVAP
                     VLEEIANDKVGALKVAKIDVDANPATARDFQVVSIPTMILFKDGAPVKRIVGAKGKAA
                     LLRELADVV"
     gene            complement(1384..2364)
                     /gene="trxB"
                     /locus_tag="VFC01_16535"
     CDS             complement(1384..2364)
                     /gene="trxB"
                     /locus_tag="VFC01_16535"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZN81275.1"
                     /translation="MTSSSTVHDVIVIGSGPAGYTAAIYAARAQLTPLVFEGTQFGGA
                     LMTTTEVENYPGFREGITGPELMDEMREQALRFGADLRMEDVDAVSLEGPVKTVTVGD
                     ETHHARAVILAMGAQARHLGVPGEEALIGMGVSTCATCDGFFFRDQDIAVVGGGDSAM
                     EEATFLTRFARSVTLIHRREEFRASKIMLERARVNEKIKFLTNTQVISIEGDPKVTGV
                     RLRNTITGEESKLAVTGVFVAIGHDPRSDLVRGQVDLDPEGYVLTKGRTTSTSVEGVF
                     ASGDLVDHTYRQAITAAGTGCSASIDAERWLAENASATTDDNTDLIGAPL"
     gene            complement(2447..3169)
                     /locus_tag="VFC01_16540"
     CDS             complement(2447..3169)
                     /locus_tag="VFC01_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81276.1"
                     /translation="MDNSAGHEPGPITTELLADLQAGLLDDAMAAALRQRIRTDPAAA
                     AKLAALDQVRLDLAKLGAAEASAPEVPAAVTARIGAALRADRHAPAHSVRHTPRWHVL
                     ALVAGVAAAVVGMVVGGVMLVRNPAPTWSTGPTAKSITVSSPATDLPLSAPQLAGLLS
                     QRPDYGPLADPRRRADCLSGLGYSAATAVLGARPVDMRGRPGVLMLLPADTPGMVHAL
                     VVEPGCNAAHTGLLANTLVTRP"
     gene            complement(3246..3827)
                     /gene="sigM"
                     /locus_tag="VFC01_16545"
     CDS             complement(3246..3827)
                     /gene="sigM"
                     /locus_tag="VFC01_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigM"
                     /protein_id="HZN81277.1"
                     /translation="MGHFGGIDRSDRELLAAHVAGDQYAFEELFYRHHRQLHRLAMVS
                     SRNPEDAADALQDAMLKAHRSAPSFRHDSAVSSWLYRIVVNACLDRLRRHKAYPTAAL
                     EDDACHVGDPAPRVETAIVVERALMRLPIEQRAAVIAVDMQGYSVAETARLLGIAEGT
                     VKSRCSRARAKLAEALEYFNAGAAAIADRAGEP"
     gene            complement(3895..>7381)
                     /locus_tag="VFC01_16550"
     CDS             complement(3895..>7381)
                     /locus_tag="VFC01_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HZN81278.1"
                     /translation="NQLPNLIAALVLEATFTAIFVPVLARAERDDPDGGTAFVRRLVT
                     LATALLVVVTLLSVAAAPLLVRLMLGSDPQVNQPLTTAFAYLLLPQVLFYGLSSVFMA
                     ILNTRNVFGPPAWAPVVNNIVAIATLGLYVLVPGELSVDPVEMGNAKLLVLGIGTTLG
                     VFAQTAVLLVAIRQERVSLRPLWGVDDRLKKFGAMAAAMVLYVLISQIGLIVGNQIAS
                     EAAASGPAIYNYTWLVLMLPFGMIGVTVLTVVMPRLSRNAAADDVPAVLADLSLSTRL
                     TMVTLIPIVAMMTVGGPAIGSALFAYGNFGEVDAGYLGMAITLSAFTLIPYALVLLQL
                     RVFYAREQPWTPILLIVVITTVKIVASLIAPYMTDDRELVAGYLGLANGLGFLAGATV
                     GYFLLRASLNPPGGRLISLAVVRTILVTITASLIAGLVAHVVDQLLGLESLTRNWGGA
                     GSLLRLLVVGLIMVPIIAGVMLAARVPDAEAALAAVRRRLRRGAGPTLEGRGRVAVPP
                     TAPPDRPRPGRPLTYADQRNSLASRQSQFPAAARRGPPASVAGDWMRKGPAVTDDQAT
                     GAAAVPDSTATTKIPRPSADDFAPDVPVDGIEPDVSEETAAFPEDTAAFAEDTAAFAE
                     PANGTAPMPVSGRPPADYGGDPTREPIAFAPPHEPAIESATANEDVHLVPGATIGGGR
                     YRLLVFHGGPPNLQFWQALDTALDRQVALTFVDPDATLPDEHVQAILSRTLKLSRLDM
                     PGVARVLDVASTGSGGLVVSEWIRGGSLAEVAETSPSPIGGARAIQSLAAAAEAAHRS
                     GVALSIDHPSRVRVSIEGDVALAFPATLPDATPEDDIRGIGAALYALLINRWPLPESG
                     VRSGLAPAELDAAGQPIEPRSVDRDIPFQISAAAARAVQEGGGIRSAPTLLNLLQQAT
                     AIADRTELIAPVDEPEVPPPSRFRGAPSDDPESAARRRKGLMIGLGVGGAIILVALIL
                     LASVLSRIFGDVGNGLGGDQLGLNAPTTSAQGDGGGGGDGNAVKPVRVTVFSPEGEAD
                     APDEASKAIDGNPATVWPIDTYTDPVPFPNFKNGVGLMLQLAQPTKIGEVTIDLNSTG
                     TAVQIRSSQTPTPASLDDTTVMVNATTLKPGSNTIKVDDATPTSNVLVWVSTLGQVDG
                     KSRSDIAEVTLKAAS"
BASE COUNT         1191 a         2463 c         2571 g         1156 t
ORIGIN      
        1 acctctcgct ggatgaagtc ggcgagatta cgcccgatcg tcgacacgga accgtgtgag
       61 ttgccgaagt gaaacgatgc gacaccgttg gcggcggggc tggccagggt ggcgcagcgc
      121 aggctgatca tcaggtcggc accgacggtg ttggcggtgt cggcacgctc ggcatcggat
      181 gggctgcgat tggcgggccg cgacaggaag gtctccatgc cgatcgccgt catccggcct
      241 tccagtcgag tcgccaagtc ccacaggatg tctgcctcgc tgatgggccc cgccggaccg
      301 ttcatgatca gcccgtgatc gttcccgccg cggccgggat cgatgatgat ccgcttgccc
      361 gagagccgcg gccccgagcg gcggaccaac tcctcttcgc ggatcgcgtg aggcgagccg
      421 ccggtaaccc gcgagcccag aaagtacaag gagcgcaacg tttccgggcc gcagatgccg
      481 tcggggtaga ggccgtactc gcgctggtac gacatcagcg cgttgtgcgt ctgcagaccg
      541 aagtggccgt cgacgagccc ggtgtagaag ccgaggtcct gcagcctggc ctgcaacgtc
      601 gcaacgtcgt cgccgtacat cggcgcaccg aactggtgat tgagcgtgcg ggcccccaag
      661 cggtacgaag cttctttcag cgcacggaaa gtcgcctcgc cgacgatgcc gtcgaccagc
      721 agcccgcgat gctgttggaa ggcacggacg gcgtgatcga gctcgtcgtc gaacacctcg
      781 acggcgatct gcctaccggt gttcaggtct tcgtcgggat tgtcgatcat cgccagcgcc
      841 gacagcgccg cccggatctc ggcaaccgca cccccgcggt caccgcgacg cagactcgac
      901 atactcggcc ctcccggctg cctttcggat cgtcggtcgt cagcgggaca aaaatcactg
      961 acgaagcaaa gacaggctag gcagcattgt cgcagacagt tctgggtttc gagaaaaccg
     1021 caggcgggtg tccggcccac ggattgcggc gttagaccac gtcagcgagc tcgcgcagca
     1081 gcgcggcctt gcctttcgcg ccgacgatcc gcttcaccgg ggcgccgtcc ttgaacagga
     1141 tcatggtcgg gatcgacacc acctggaaat cgcgcgcggt ggcggggttt gcgtccacgt
     1201 cgatcttggc caccttcagc gcgccgacct tgtcgttggc gatctcctcg agcaccgggg
     1261 cgaccatctt gcacggcccg caccaggtgg cccagaagtc gaccagcacc ggggtgctac
     1321 tggcgagcac gtcctcggag aacgagtcgt cggttacggc gactgtggca gaagtcttct
     1381 cggtcatagt ggggctccaa tcagatcggt gttgtcatcg gttgtggcag aagcgttttc
     1441 ggccagccag cgctcagcgt cgatggacgc cgaacagccg gtgcctgcgg cggtgatggc
     1501 ctgtcggtag gtgtggtcga cgaggtcgcc tgacgcgaac accccctcca ccgaggtgct
     1561 ggtggtgcgg cccttggtga gtacgtagcc ctccgggtcg aggtccacct ggccgcgcac
     1621 caaatctgac cgcggatcgt ggccgatcgc gacgaacaca ccggtgacgg cgagtttcga
     1681 ctcttcgccg gtgatcgtgt tgcgcaaccg cacaccggtg accttcggat cgccctcgat
     1741 cgagatcacc tgggtgttgg tgaggaactt gatcttctcg ttgacgcggg cacgctccag
     1801 catgatcttg gaggcgcgga actcttcgcg gcggtggata agcgtcacgc tgcgcgcgaa
     1861 gcgggtcaaa aaggtggctt cctccatcgc cgaatcgccg ccgccgacga ccgcgatgtc
     1921 ctggtcgcgg aaaaagaagc cgtcgcaggt ggcgcacgtg ctcacaccca tgccgatcag
     1981 cgcttcctca cccggtacgc cgaggtggcg ggcttgcgcg cccatcgcca ggatcaccgc
     2041 gcgcgcgtgg tgtgtctcgt cgccaacggt gacagtcttg acggggccct ccagcgagac
     2101 cgcgtcaacg tcctccatcc gtagatccgc accgaagcgc agcgcctgct cgcgcatctc
     2161 gtccatcagt tcgggtcccg tgatgccctc gcggaagccg ggatagttct ccacctcggt
     2221 ggtggtcatc agcgcaccac cgaactgcgt gccttcgaat accagcggag tgagctgagc
     2281 gcgggccgcg tagatggctg ccgtgtagcc ggccggacct gagccgatga cgatgacgtc
     2341 atgaacggtg gatgaggagg tcatgcgagc ctttctgccg tttcacgtcg gcacgggtac
     2401 aaacaccagc gtatgccccg gtgttccccg tggcctgcag gacagattac ggacgagtga
     2461 ccaacgtatt ggccagcagg ccggtgtgag ctgcattgca gcccggctcg acgaccagcg
     2521 cgtggaccat ccccggcgtg tcggcgggca gcagcatcag caccccgggc cggccgcgca
     2581 tgtcgaccgg tcgggcgccg agcacggccg tcgccgccga atagccgagt ccggacaggc
     2641 agtccgcgcg tcggcgcggg tccgcgagcg gcccgtagtc gggccgctgg gagagcaggc
     2701 cggcgagctg tggagccgac agcgggaggt cggtcgccgg gctcgacacg gtgatgcttt
     2761 tggcggtcgg tccggtagac cacgtcggag ctggatttcg caccagcatc accccgccga
     2821 cgaccatgcc cacgaccgcg gccgcgacac cggccacgag cgcaagcaca tgccagcgcg
     2881 gggtgtgccg aaccgaatgc gcgggggcgt ggcggtcggc tctcagtgcg gcgccgatgc
     2941 gggcggtcac ggccgcgggt acctcgggcg cggaggcttc ggcggcgccg agcttggcca
     3001 ggtcgaggcg gacctggtcg agtgccgcca gcttggccgc agcagcgggg tcggtgcgaa
     3061 tccgttggcg cagcgcagcc gccatggcgt cgtcgagcag gccggcctgc agatcggcca
     3121 gcagctccgt tgtgatcggt ccgggttcgt ggcccgcgct gttgtccatc gctgcccccg
     3181 ccggttgtgc cgcgccctga cgcaagccag tgtccaccac ggactgttag acgcagccac
     3241 cgccgtcacg gttccccggc acggtcggcg atcgcagccg cgccggcgtt gaagtattcg
     3301 agcgcttcgg ccagcttggc gcgcgcacgc gagcagcggc tcttgaccgt gccctcggcg
     3361 atcccgagca ggcgcgcggt ctcggccacc gagtagccct gcatgtcgac cgcgatgacc
     3421 gcggcgcgct gttcgatcgg cagcctcatc agggcacgct cgactacgat cgcggtctcc
     3481 acccggggtg ccggatcgcc gacgtggcag gcgtcgtcct cgagggcggc cgtggggtag
     3541 gccttgtggc gccgcagccg gtcgaggcag gcgttcacca cgatccgata cagccagctg
     3601 ctgaccgccg aatcgtgccg gaagctgggc gcactgcgat gcgccttgag catcgcgtcc
     3661 tgcagcgcgt ccgcggcatc ttcgggattt cgactgctga ccatggcgag ccggtgcagc
     3721 tgccggtggt gacggtagaa caactcctcg aacgcgtact ggtcgccggc gacgtgtgct
     3781 gccagcagct ctcggtctga tcgatcaatt cccccgaagt gccccacagc cgaacactaa
     3841 acggccccag aggtgctctc ttgcacaagt ttgccgggct ggggatagcc cgcgtcagga
     3901 cgcagctttg agcgtgacct cggcgatgtc ggaccggctc ttgccgtcca cttgacccag
     3961 cgtggaaacc cagaccagca cgttggacgt cggcgtcgcg tcgtccacct tgatcgtgtt
     4021 cgagccgggc ttgagggtag tggcattcac catcacggtg gtgtcgtcca gcgaggcggg
     4081 agtcggcgtc tgcgatgagc ggatctgcac tgcggtgccg gtgctgttca ggtcgatggt
     4141 gacctcgccg attttcgtgg gctgcgccaa ctgcagcatc agcccgacgc cgttcttgaa
     4201 gttcgggaac ggaacggggt ccgtataggt gtcgatgggc cacacggtcg ccgggttgcc
     4261 gtcgatggct ttgcttgcct cgtcgggcgc gtcagcctct ccttcgggtg aaaacaccgt
     4321 cacccgaacg ggtttcacgg cgttgccgtc accgccgccg ccgccgtcgc cctgcgccga
     4381 cgtcgtcggt gcgttcaggc ccagctggtc accgccgagg ccgttgccga cgtcaccgaa
     4441 gatccggctc agcaccgacg ccagcaggat caatgcgacg aggatgatcg cgccacccac
     4501 gcccagcccg atcatcagcc ccttgcggcg gcgcgccgcg gactcgggat cgtcggaggg
     4561 tgcaccgcgg aagcgggacg gcggcggcac ctcgggttcg tcgaccgggg cgatcagctc
     4621 tgtgcggtcg gcgatcgcgg tggcctgctg cagcaggttc agcagggtcg gggcgctgcg
     4681 gatgccgccg ccctcctgca ccgcccgcgc ggccgccgcc gagatctgga acgggatgtc
     4741 gcggtcgacg gagcgcggtt cgatgggctg gcccgcggcg tcgagttcgg cgggcgccaa
     4801 tccgctgcgc accccggact cgggcagtgg ccagcggttg atcagtagcg cgtagagcgc
     4861 ggcgccgatg ccccgaatgt cgtcctcggg agtcgcgtcg ggcagcgtcg ccgggaatgc
     4921 cagcgccaca tcaccctcga tgctcacccg cacccggctg ggatggtcga tcgacagtgc
     4981 gacgccggag cggtgcgcgg cttcggcggc cgcggccagc gattggatgg cccgggctcc
     5041 gccaatcggc gacggcgacg tctcggcgac ttcggcgagc gagccgcccc ggatccactc
     5101 cgaaaccacc agcccgccgg agccggtgct ggccacgtcg agcacccggg cgacgcccgg
     5161 catgtccagc cgactcagct tcagcgtgcg agacaggatc gcctgcacgt gctcgtcggg
     5221 cagtgtggcg tccggatcga cgaacgtcag cgccacctgc cggtccagtg cggtgtcgag
     5281 cgcctgccag aactgcaagt tgggcggccc gccgtggaag accagcagcc ggtagcggcc
     5341 gccgccgatc gtggcgcccg ggacgaggtg gacgtcctcg ttggcggtgg cggactcgat
     5401 cgccggttcg tgcggcgggg cgaatgcgat gggctcccgg gtggggtcac cgccgtagtc
     5461 ggcgggcgga cgtccggaca ccggcatcgg cgcggtcccg ttggcgggct cggcgaacgc
     5521 ggctgtgtct tcggcgaacg ccgcggtgtc ctcagggaac gccgcggttt cctcggagac
     5581 gtcgggttcg atgccatcga ccggcacgtc gggcgcgaag tcatcggccg acgggcgggg
     5641 gatcttggtg gttgcggtgc tgtcggggac cgcggcggcg cccgtcgctt ggtcgtcggt
     5701 caccgctggc cccttcctca tccagtcgcc ggcaacgctg gccggaggcc cgcgccgggc
     5761 cgccgccggg aattgacttt ggcgtgacgc gagcgaattc ctctgatcag cgtacgtgag
     5821 ggggcgacct ggccgcggcc tgtcgggtgg ggcggtcggt ggtacggcta ccctcccccg
     5881 cccttcgagc gtgggcccag cgccgcggcg gagccggcgc cgcacggcgg ccagggcggc
     5941 ttccgcgtcg ggtacccggg cggccaacat cacccccgcg atgatcggca ccatgatgag
     6001 cccgaccacg agcagccgca gcagtgagcc tgcgccgccc cagttgcgcg tcagcgactc
     6061 gagcccgaga agctgatcca cgacgtgcgc gacgagcccg gcgatcaggg acgccgtgat
     6121 cgtcaccaga atggtgcgca ccaccgcgag gctgatgagc cgaccgccgg gcggattcag
     6181 gctggcgcgc agcaggaagt agccgaccgt ggcgccggcc agaaagccca gcccgttggc
     6241 cagcccgaga taaccggcca ccaactcccg gtcgtcggtc atatagggcg cgatcagcga
     6301 cgcgacgatc ttcactgtcg tgatgacgac gatcagcagg atcggggtcc acggctgctc
     6361 gcgggcgtag aacacccgaa gctgaagcag cacaagggca tacgggatca acgtgaaagc
     6421 cgacaaggta atcgccatgc cgagatagcc ggcgtcgact tcaccgaagt tgccataggc
     6481 gaacagcgca ctgccgatcg cggggccgcc gacggtcatc atcgcgacaa tcggaatcag
     6541 cgtgaccatt gtcagccggg tggacaaaga cagatcggcc aacaccgcgg ggacatcgtc
     6601 ggcggcggcg ttgcggctca gccgcggcat caccacggtc agtaccgtca cgccgatcat
     6661 gccgaacggc agcatcagca ccagccaggt gtagttgtag atcgccgggc cggaagccgc
     6721 tgcctcactg gcgatctggt tgccgacgat cagaccgatc tggctgatca acacgtacag
     6781 caccatcgcc gccgccatcg cgccgaactt cttgagccgg tcgtcgacgc cccacagcgg
     6841 acgcaggctg acccgctcct ggcggatggc gaccagcagc accgcggtct gtgcgaacac
     6901 acccagcgtc gtgccgatgc cgagcacgag cagcttggcg ttgcccatct ccacgggatc
     6961 gaccgaaagt tcgcccggca caaggacata caggcccaga gtcgcgatcg cgacgatgtt
     7021 gttgacgacg ggcgcccagg caggcggacc aaacacgttg cgggtgttga ggatcgccat
     7081 gaacaccgac gacaggccgt agaacaacac ctgcggcaaa agcagatacg cgaacgcggt
     7141 ggtcagcggt tggttgacct gcgggtcgct tcccagcatg aggcgaacca gcagaggcgc
     7201 ggcggccacc gacagcagcg tcacgaccac cagcagcgcg gtggccagcg tgacgagccg
     7261 ccgcacgaac gcggtgccgc cgtcggggtc atctcgctcg gcacgcgcca gcaccggcac
     7321 gaaaattgcg gtgaacgtcg cttcgagcac gagggcggcg atcaggttgg gcagctggtt
     7381 g
//
LOCUS       DASRJA010000375        25573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1703487,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000375 DASRJA010000000
VERSION     DASRJA010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 25573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25573
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703487"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1458)
                     /locus_tag="VFC01_16555"
     CDS             complement(280..1458)
                     /locus_tag="VFC01_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZN81279.1"
                     /translation="MTSLLTDKVIISGIGQSDIGRRLGRDALELTADACVAAVEDAGL
                     TLADIDGLTTYPGASQAGPGFSGASLRDIHDALGIKPNWVAGGVESPGQLGAVVDAML
                     AVAGGMADHVLCWRSIWEGTAQGAGRRQGYGARGGRPTGMMAWQLPFGATAANLAAMQ
                     VRARMIRYGLTREQLASIAIVERANARLNPSAIYSDPLDLESYLSARMVSDPLCMYDC
                     DIACDGATAFVISRVEHAAALDHPSVTVEALDCAHHDRFMWEGGEDITRISSRWSKIW
                     ERTDYTVTDVDYASLYDGFSVFVLCWLEDFGFCKVGESGEFIADQARISLGGDLPINT
                     AGGQLSGGRLHGFGHLHEACLQVRGEAGDRQVDDVALAAVGVGAANSGTTAMLLRSAR
                     "
     gene            complement(1458..1721)
                     /locus_tag="VFC01_16560"
     CDS             complement(1458..1721)
                     /locus_tag="VFC01_16560"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZN81280.1"
                     /translation="MPEYVALSGRGIVETWTVNQHQFFPGFTPPYVIAFVTPVEDDRV
                     RVLTNLVNVEPDDVTAGMPVRVTFQRCVDGEDEVFIPLFEPDH"
     gene            complement(1737..1880)
                     /locus_tag="VFC01_16565"
                     /pseudo
     CDS             complement(1737..1880)
                     /locus_tag="VFC01_16565"
                     /inference="COORDINATES: protein motif:HMM:NF023594.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(1877..3259)
                     /locus_tag="VFC01_16570"
     CDS             complement(1877..3259)
                     /locus_tag="VFC01_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZN81281.1"
                     /translation="MTDLATERVPEKYACGETFVPKSRYIDPDFLRLELDRLFTQVWQ
                     VACREEEIPDAGCYYEYSIGRQSIAVVRQKDHSIKAFYNTCAHRGMKVVRGSGCAVDG
                     DLRCEFHGWRYALDGRSSFVPCREEFASRPREQWGLTPINVGTWGGWVFISMADDPPD
                     LLDWLAPLPTALAPFRLQDMRYRWRKRTFLPANYKTVVDAFIEGYHTPGTHPQTLRAA
                     QGPRPTTEPALPQEYAYAPYTPTITYDNHSRFIYTQRPESAGRDRARQQQAARPEVFA
                     NSMQYNYLEVGSLVTERDYRAAQQLATMKPSEVPPFVLYHKMCEELARADGVDFPKMS
                     MEEYFAGNGDWHVFPTLVILVEKSCLLGYRMLPDADDPNRCTFEMFSLEHFAPGEVPE
                     TSWQEFERWQDHDGWGELPTQDLKNIGAIHAGMHSKGFNGLWLNTAQEMSIRNEHAIA
                     DRFIFGADEQ"
     gene            complement(3278..4183)
                     /locus_tag="VFC01_16575"
     CDS             complement(3278..4183)
                     /locus_tag="VFC01_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN81282.1"
                     /translation="MGALDGRTALVTGAGRGIGAAIAEGLAAEGATVLVSDAGVGVDG
                     TGHDAGPAEAVVAAINERGGKAFPDATDITDFAACADLIDRAANTLGGLDVVVNAAGI
                     LRDGMVFKMSEQDWDAVVAVHLKGTFNITRHAAGWWRENRGGQYRLINFTSMSGLQGA
                     PSQPNYAAAKMGIVGLTFSCANALRNYGVTSNAIAPIAGTRMTQGIKGGGSMDYSDSN
                     VRLSPKNCVPPVAYLASTQSDWLNRRVIFAGNGRISLMSNPVVEREIVSATGTWDIPT
                     AFAEIETSFKEAVLWPNIFDKPPAS"
     gene            complement(4257..5147)
                     /locus_tag="VFC01_16580"
     CDS             complement(4257..5147)
                     /locus_tag="VFC01_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN81283.1"
                     /translation="MSTDDQTALAVDEDWVRYAVIEPHIAQITINRPERRNAILSPDM
                     HALFRDRLDRAEEDDDIKVVILKAEGKDFCSGDDVRRLPVEQAGLTKDKRLPQTARMA
                     NARRLHRHLTTWLEFPKTVIAACQGATLGAGMNLALAADILVVSEDMYLARPQARIGF
                     AGFSTAMPLALLKLGPNRGYEAMITGRKVSASELCDWGVAASVVPVGGLDDEALRYAR
                     AIAHHSADGLMVGKHALIAFWHAVGMAQFGDWVPMAHSVFSNLSWREDEFNFMKERAA
                     RGGREALAELERRYSEWGFE"
     gene            5230..5658
                     /locus_tag="VFC01_16585"
     CDS             5230..5658
                     /locus_tag="VFC01_16585"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN81284.1"
                     /translation="MPTMTVSKDISARADAVWAILANFGEVGWIPMDINVEVEGDGPG
                     MRRKIQGSGDDATVETLVWIRPEQKRLAYEITNNPLPVNRFETVVFVADSAVAGDGCR
                     IGWEVDYEPSGDDASARESIELVYGMMAGWIEDAATSAGA"
     gene            5694..7013
                     /locus_tag="VFC01_16590"
     CDS             5694..7013
                     /locus_tag="VFC01_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN81285.1"
                     /translation="MLSVIDPLDLVLISVDDHTVEPPDMFKGRVPSRFADDAPKIVQG
                     NDGVCFWEYAGLQMPNIGLNAVAGRPNDEWGFEPSRFEDMRTGCYNVDARVDDMNASG
                     VLASLCFPSFPTISGALFTYHGDRDISEVMVRAYNDWHVEDWCGRHPDRFIPMGILPL
                     WDPVLAAGEVARLAGAGCHAVTVPQHIGSYGQPPWQDPQWDVLWESVCEHDTVVNIHI
                     GTGGGVPVPSDQTTYLAYNSMLAIDTMRFTADLLFSPVVVKFPTIKFALSEGGIGWIP
                     FLLERFEDIYSRQRAWTGDDLGSGLTPTDVFRRNFMSCFIRDRVGIEMRDRIGVETIC
                     WEMDYPHSDSSWPDAPEQLAAQLEGCSPDEVEAITWRNAARAFGYDAVERLGRENCTV
                     AALRDRIVGMDLSAPKVDAGRAPKAGTTAITYGEMKERMASIIRGGT"
     gene            7010..8203
                     /locus_tag="VFC01_16595"
     CDS             7010..8203
                     /locus_tag="VFC01_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN81286.1"
                     /translation="MTDRDYGVVLNDADEAFRDEVQSFLSSSLTPDLVSREGEETDHL
                     DRMQLWQKRLHAAGLAAISWPVAYGGRAATPTQQLIFNAEMAAAHAPEPINRSAINQL
                     GPTIIQWGTDEQRALYLPRILSAEDVWCQGFSEPEAGSDLAALKTKAVIDGDELVITG
                     QKIWTSKASYADWIYILARTDSTAAKRDGISYILADLNSPGIEVRPIRQITGAAEFNE
                     VYFDAVRVPLSNVLGPLHGGWKVAKSTLGYERVGQSRTHRIERRLDILVKMAQQPNAL
                     TDKGLADSYVADRIARYAAQVEALRQIAAQATAAGVRGVSPGPEASVAKLLTSEVDQS
                     MATFGLELAGPAGTLERGSAGAAKKGNVAQSYLLMRAATFGAGTSEIQRNVIAERLLG
                     LPRDP"
     gene            8193..9272
                     /locus_tag="VFC01_16600"
     CDS             8193..9272
                     /locus_tag="VFC01_16600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN81287.1"
                     /translation="MTPDTAISPTVSLDEIADTAAELAELRRSADDILRQAWSVDRSR
                     ALLDGPGPAFDEALWTTVTDLGWPDVLVSEDNGGAGGALRELAVLVEAAGAAAAPIPL
                     AATAAAGWSEDRCSDGVAVLLPESGTLAGQMVSGTFNFVPYGAVATRLVVLAEDNRQS
                     LLGVVDPNGMGVKRAPLKPLDHGPAALITLDGAPLRVITDGADAVRRHRDALLRLRVG
                     LVAELVGVAAAANDAATEYAKVRVAFDRPIGSFQAIKHRLVDQRSAIEVGRALVNRAA
                     DACDQHHPDATALVSLAAFWAIDSLRAVPEGATQVFGGIAYTWEHEAHVHLRRAATLS
                     ASLGPRAEHRTAITDWLAARSGVQR"
     gene            complement(9263..10477)
                     /locus_tag="VFC01_16605"
     CDS             complement(9263..10477)
                     /locus_tag="VFC01_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN81288.1"
                     /translation="MATITGISEARFDDPGFYLGDPNATFRELRETDPLHWYEEGKFW
                     VVTKYDDIKTISARPEKFTSERIAIMMDLIAHRQGVDPQGFGSRGIMFMDPPEHRAHR
                     KAIGVRFTPAAVAKMEDRVKQVVAQAFDNLPDGEFDWIEHIAEPIPVYIFSYFLGVPE
                     SDWSKVASWATTIAKAGSGLASDEDFQVIFDQIAPYLLGLAAERAESPRDDLLTMLTQ
                     VRIDDEPFNEVQIMVYAMTLLAAGSETTQSLLAGLIECLDVHPAAADALFADSSLSDN
                     TVEETLRWWTPVMSMARQAAHDVELRGKKVLEGQGLLMAYASANRDVDHWGPTAEDFD
                     ITRSDAAGHVGFGVGEHFCMGAALARREARFVLAEVLRRAKAVHVTGQRIKRESSLVN
                     TFDRLDVTLDYR"
     gene            10610..12688
                     /locus_tag="VFC01_16610"
     CDS             10610..12688
                     /locus_tag="VFC01_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN81289.1"
                     /translation="MSHNPSTTDFLADVRVLQLGDGIAGSAAAALLASLGADVAKISG
                     EKVRRAGPVLTGPQGITPAVDFVLDHRKRMLDPGADPVQHARAADIVIVDGAAVPSVD
                     GPTVVVTLSPFGSDEPGTGHPGGELVAQASGGLLATIEDGDGRPVPAPGYVALKSAGA
                     VAALAALHGLDRRNDTLLPVHVDVSVQEAVIYTAALPECAHIMYRCPGRAGSGRYLAP
                     SGVFACRDGLVRITAVENHQWKGLLVALGNPAWAVGLDDRPARIEHAELINAKVTEWT
                     TGRAKGDCAALLQANGVPSTPVNLPGELLSSPQLAHRSALTKAPMGDRHVVVLGTPWR
                     TELGNAGQRRRGGLRGLRIAELTHVLAGPIIGSLLGAMGATVVRLEDPERLDIYRRSG
                     PFADGVAGVERGAYYAVANHSKRSVLIDPGGAVEDVAAALSEADVLIENVGSSRLDRL
                     HVSPAELAESGRLAVRVSGFGSDGPLAGYRVYANNVQAYGGLAGLTTGPDGEPARLGT
                     VLADPLSSVVAAVVIAAWALGPHSDTGAVVDVSMSEVVASTISEYVAAASVDQAAPDP
                     TVRRLVYRSADSRWVAVELVGDESRPYNDGELADRIAAADAEDVAAQLAGAGVRAAVV
                     RSAGEVVTDERLAARGFFPEITHPDPDLGTARLVGLPWRFAGEGPVPLAPPPALGSTS
                     TSALYERTAV"
     gene            12691..12924
                     /locus_tag="VFC01_16615"
     CDS             12691..12924
                     /locus_tag="VFC01_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81290.1"
                     /translation="MSGFQTPATVWSSTFVPSKSPFPEFGQDGYAVAWVDTEDGRFQV
                     LVDGARPVPGTVGRLVSATLGEDAIEMFVADPT"
     gene            12921..14039
                     /locus_tag="VFC01_16620"
     CDS             12921..14039
                     /locus_tag="VFC01_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZN81291.1"
                     /translation="MTGVHITGVGLTGFGKHSDTPLADLGVAAAENALADAGIGYDAV
                     GEVFTSSALAGPQTGLAVAHRLGRTGIPVTATESASAGGMVALRHAVWAVASGRCSTA
                     LAIGYEKTTALEPGGVVPAAVGFWDHFPPQLHYAIEAAHWLYETQCGPEVIAAVAAKS
                     YNQARLNPLAARRNPERVTVDDVLGARVVAEPLTKMMCHASVDGGAAVVVSADTAPRS
                     VSVLSIEQTSWPKDPGWPLVGPVVGPPSQITLTAQRAYANAGVGPQDVGVVSLHDMCA
                     SEEITALIAMGLTDADGVVKLAESGGLASDGSLPTNTDGGCIARGHPIGATGLAQTAE
                     VVSQLRGEAVSRQVAEPRTGVVQAVGGGGSCVVALLRG"
     gene            complement(14043..15251)
                     /locus_tag="VFC01_16625"
     CDS             complement(14043..15251)
                     /locus_tag="VFC01_16625"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN81292.1"
                     /translation="MKGVRVLEVAQWTFVPAAGAVLADWGADVIKIEHPRTGDSQRGL
                     RNLGHITIEGNRNPVMEHANRGKRSVALDMSTPDGHELLMDIVRTSDVFLTNFLPDAR
                     EKLRIDVDDVRAVNPKIIYARGSAYGPLGAEAGTGGYDMTGFWSRAAGALGSTPSDLE
                     GVVPQPGPAYGDSIGGMTIAGGISAALFERERTGHARIVDISLLGVGVWASGVAINAA
                     LVSGQPWVANPAGANVAPNNPLVGFYGTSDRRFLGLSMLQGFRFFGEFCRRIGVGELA
                     ADERFASHELLARNAPAATEVLRQAIAAHPLEHWRKALDGFDGQWSVVQNTVEVAADP
                     QVRANGGIVGIETESESFEIVASPVLFDETPLHLSPMPEFAASTEELILELGGDWDRI
                     MALKESGAIA"
     gene            15317..16540
                     /locus_tag="VFC01_16630"
     CDS             15317..16540
                     /locus_tag="VFC01_16630"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN81293.1"
                     /translation="MTGALSHLRVCDLSGQLAGAGATKILAAFGAEVIRVEDPATQGL
                     WDALRGVGPYVDDRRGVNFGAGFNNHNVGKYGVTINLRTPEGKELLRELISVSDVVCE
                     NFAAGVMENRGFGYAELQRIKRDIIYVSNCGFGHTGPYRDFKTWGPIVQAVSGLTYTA
                     GLPDTEPSGWGFSYMDHGSAFYMTTAILAALHHRDKTGEGQHVDLATVSAGIAMLPTE
                     ILDWTVNGRSTAAGGNRADFGEMAPHGIYPCLGDDRWIAMACRDERELALLAKVLNEP
                     ELASDRFSTLGQRLQAVDELDQMISAVTTTRDAAALAEDLNAAGVPASVVKSPAERID
                     GDPDLAAMELFPTVTHPDIGDVRVEGIPMRFSATPWQITSAAPRVAQHNREVLKDLLG
                     HSDAELDDWTRRGVI"
     gene            16546..17766
                     /locus_tag="VFC01_16635"
     CDS             16546..17766
                     /locus_tag="VFC01_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN81294.1"
                     /translation="MQDLRVIEIADQFTPVGGRVLAELGAEVIVVEPPSGSPHRSRPP
                     FVDDEPGIDRSLRWWAGNVGKRSVTIDLTTEAGARTLEDLIATADIVIAGSDQCEGTH
                     IDYQRCAARDGRLIWISVSPFGLRSARADQPVTDLTMLAGGGPVWNCGYDDRTLPPIR
                     GAGDQSANIAGMYCAIGTLVALSHRDHTEQGQLVDVNVTAACNVTCEQTTYHWLVNRD
                     VCLRQTGRHAYPTPSSKVQVRCADGHYATTGVLPRKPEEFARLSSWLGELGLTEELPE
                     AVFLDLAAERDEPVDLALIGTDDETTAILSAAREAIRLIGSRLPAKEFFVTSQRRGFP
                     CGAVLSPDEAFEDEHTVARGFPVPVAHTELGRTVTYPGTPYVFSATPTVTPTRPPLLG
                     EHNDLIAELVAQWP"
     gene            17763..18218
                     /locus_tag="VFC01_16640"
     CDS             17763..18218
                     /locus_tag="VFC01_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN81295.1"
                     /translation="MNNGHRLTHFGLCVRDIGRSTEFYCAALGFTEIGRMRVDDDASA
                     RLLDVPDLVLDLVYLHRDGFRLELLGYARPGTIGEPAPEPMNRLGFTHLSFRVDDVDG
                     MIATIVEHGGRALADRTVKFDGGNRGVMATDPDGNLLEFIERIPRLTGS"
     gene            18246..19418
                     /locus_tag="VFC01_16645"
     CDS             18246..19418
                     /locus_tag="VFC01_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN81296.1"
                     /translation="MYSPFSKTIFDDPYPVYRRLRDEAPVYRDPEDRWWVLSRFDDVA
                     GALRDWPTYSSKLGPAPENPDDDGRKYSVISMDPPRHDRIRGVLKGFFTPKAVAAMED
                     ALQTVVTHHLDRLRPGTTVDAMEAFAFSVPTDVIGDLLGVPKADREQLRVWWEAFLTR
                     HEGEVAMPPSAIEANRKISAYIGDLIEERRSNPGEDLISIVLRATFDDPEVGGERSLT
                     PHEVLMFANLLSAAGSETTQKLISNGLVALHDHPDQWQRIVNDHSLIPAAVNEALRYD
                     TPSHWVARTLTRPVEKHGVTMNAGDWVLLLLGSANRDERRYDSPDRFIINRPRGTDVY
                     FGWGIHICLGQWLARREAQLVFEYVAKKFPNYTVGAHERVLTATVRGYTSVEMTLS"
     gene            complement(19415..20203)
                     /locus_tag="VFC01_16650"
     CDS             complement(19415..20203)
                     /locus_tag="VFC01_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN81297.1"
                     /translation="MDDRNALVTGGAQGLGRAIARRLAAEGARVTIVDLNEDKGRECI
                     GLIQSAGGTAEFVKANVAKRDDIEAATAAAAVDGRLDILVNAAQYFAMPKALELVTDK
                     DWELSEATGPKATFRFMQSAFGYLKKTGKASVINFVSGSALGGMAYTGPYSAAKGAVH
                     ALTKVAANEWARHGIRVNAVCPFALTDVQRDMIGTEWDNYTRTATASPMKRGADPDSE
                     IAPAVAFLASDDASFVTGTVLHVDGGMTELSTVDYSQSPGVFNS"
     gene            20294..20488
                     /locus_tag="VFC01_16655"
     CDS             20294..20488
                     /locus_tag="VFC01_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012835631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN81298.1"
                     /translation="MRVEVDRDLCESNAVCVGIAPDIFDLGDDDLARVLVDEVPQGRE
                     DEVREAVGLCPKIALKLHED"
     gene            20498..21541
                     /locus_tag="VFC01_16660"
     CDS             20498..21541
                     /locus_tag="VFC01_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN81299.1"
                     /translation="MNIGLTDEQKLLQDTASGLAENLATTDPSDIPSGDTLADHWRQL
                     VELGVPTLRSPSLCGLDASGVETVVVIEQLARTMCAVPVVGQAVFATELLEAAGAHKE
                     LELIAEGSLRIAPILTVDLQRFGATEAGGVAFDAAGATHALLAIESGGVHRLACAPLE
                     DTTEEGLDLTRTLRRTPADVAASATTTGEPIDADRWHRVEALALTATAADLVGVMQGA
                     LDEAVRYAGDRVQFGVKIGSFQAVQHMLADALVRVEGARSCLWHAAWAIDHLPVDEAR
                     LAAMTAKAYAAAAGRDVVETAVQVFGGIAITWEHVSHLRVRRTLLNRRLFGDEATLYE
                     AVAQMRLENEELA"
     gene            21545..22687
                     /locus_tag="VFC01_16665"
     CDS             21545..22687
                     /locus_tag="VFC01_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN81300.1"
                     /translation="MDFNDTAEEAEFRARLRQWLHDHSADAAIPDDPAARADAQNAWH
                     QTLHKAGYIGLSFPVEYGGHGLPPIYEAILNDELGRAGAPPIEGVGHLSNALRLFGSD
                     LQRAELLPGLMSGAVRWCQGFSEPEAGSDLASLKTRADLIDVDGRDVFRVNGRKIWTS
                     FAAVADWCFLLCRTEPDARKHEGISVLLVPMSTPGIDVRPIVNAARNREFTEVTFDDV
                     DVPAENLLGERGQGWSIATQLLAYERGPSDINWISRLSRQLRSLERDVRLGRLENSPM
                     TRVRLGQAYAELRALQMKVQRSLSDRQRGALPGPEGSVDKLLMARADQVVGHTMMDLT
                     DSEPLLTEGLEWDIYVWSRAAGIYGGTAQIQRNIVAQRVLGLPRTT"
     gene            complement(22689..22895)
                     /locus_tag="VFC01_16670"
     CDS             complement(22689..22895)
                     /locus_tag="VFC01_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81301.1"
                     /translation="MDPKDRIPHDDWADQDLLTRAEAAERLAIEIKEIQAKLAAGDAD
                     EVMLRRLAALEEAHAQMTGSGGAG"
     gene            23016..24314
                     /locus_tag="VFC01_16675"
     CDS             23016..24314
                     /locus_tag="VFC01_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN81302.1"
                     /translation="MPLQPSMKLLSVDDHLIEPPHVWSDRLPKKYLHDGPRIVEFPRE
                     GKPPMHQWVYEGRSYPNIGLNAVAGKSPEEFGVDPVRYDDMIPGCYDPKARLIDMDID
                     GVHAMLCFPSFPRFCGTVFLEGTDKDLALLCVQAWNDFSLDEWCAADPARFIPMMITP
                     LWDTQLMVTEIERVAAKGCRAVGLPDNPMNLGLPSFHTSHWDPVWSALEETNMTAVMH
                     FGSGGMPPSTAAEAPFAVMVTLMGTTSMAAAVELVFSPVFHKHPNLKVAFSEGGIGWM
                     PYLVERADYVWRKHKYYQNIHPTVAPSELFRRNITGCFIEDEVGVAMRHQIGIDNITW
                     ECDYPHSDSFWPKSRARAEEMLSKVPDEDAHKIVELNTRRWYAFPEEGFKASTADSGW
                     RPNDGNPPEYDYDAVMSDHGGVGYGAFIQNLADQMANKEN"
     gene            complement(24323..>25573)
                     /locus_tag="VFC01_16680"
     CDS             complement(24323..>25573)
                     /locus_tag="VFC01_16680"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN81303.1"
                     /translation="YFWLTLPADADEAHGRRTLLVVPVRMAVIHATVWTAAALLLVVI
                     NLGTPWLAATLGISVLLGAAVTAPLSYWLCARALREAVAELLTTNPPTRPRGPGLRAR
                     AVGAWVVGTGIPMMGLMLVAASALAVDYPRDRLAWVVLALGALTVACGLAVAALIGAT
                     TADPIDEVRSGMRRVERGEYDVSVPVFDASELGLLQAGFNIMAGGLRERERLRDLFGR
                     HVGRDVARLAEENAHIAMGGEKCEIAVLFIDMVGSTQLAAALSPEDVVSILNDFFAVV
                     VEVIERHGGLINKFQGDAALAIFGAPVSTDDNAGRALAAARELGSRLNPSSTEIKFGI
                     GVSAGKAAAGNVGGHSRYEYTVIGDPVNEAARLSDVAKESGGVVASAAALQRASDSEA
                     RRWHVVGQQVLRGRDTATEIATPS"
BASE COUNT         4793 a         8204 c         7931 g         4645 t
ORIGIN      
        1 acgatgccgt atgggcgtgg tcgcgaggca tcggttatcg cgctagcaat agcgacctca
       61 caaccgagga tcctgccggc gcattccccg tcatcggcat cggtgatgag cgtgactctc
      121 caccccgcga ggacacgatc aacaatccac ccgccggctt ctgccaccac ctctcggaca
      181 gagtcggaga acacgtccaa tcgatatgga ttagcgtgcg ccgaatcgtc gctgcgacag
      241 tggttttcgc tctcaggccg ttgcattcgc ctccccgttc tacctcgccg accggagcaa
      301 catggcagtg gttccgctgt tggcggcgcc cactccgaca gccgccagcg cgacgtcgtc
      361 aacctgacgg tctcccgctt cgcctcggac ctggagacag gcttcgtgca gatgaccgaa
      421 gccgtgcagc cgcccaccgg ataattggcc gcctgcggtg ttgatgggca aatcgccgcc
      481 gagcgagatg cgtgcctggt cggcaatgaa ctcgccggac tcgccgacct tgcagaaccc
      541 aaagtcttcc aaccaacaca acacgaatac gctgaaaccg tcatacaacg acgcatagtc
      601 gacgtcggtc acggtgtaat cagtccgttc ccagatcttc gaccagcgcg aactgatacg
      661 tgtgatgtct tcgccgccct cccacatgaa cctatcgtgg tgagcgcagt cgagcgcctc
      721 tacagtgacg gacgggtgat ccagggccgc agcatgttcc acacgggaga tcacaaacgc
      781 cgtcgcaccg tcacaggcga tatcgcagtc gtacatgcac aacggatctg acaccattcg
      841 cgcgctcaag tagctttcca agtccagcgg atcgctgtaa attgcggagg gattcagccg
      901 cgcattcgct cgctcgacga tcgcgatcga cgcaagctgc tcgcgagtca gcccgtatcg
      961 gatcatccgg gcacgaacct gcatcgcggc gaggttggcc gcggtagcac caaaaggcaa
     1021 ttgccaagcc atcattccgg taggccgacc gccccgcgct ccgtaccctt ggcgccgacc
     1081 cgcgccttgg gcggtgccct cccagatact gcgccagcac aggacgtggt cagccatacc
     1141 gccggcgacc gccaacatcg cgtccacgac ggcgcccagc tgtcccggtg actccactcc
     1201 ccctgcgacc cagttcggtt tgataccgag ggcatcgtga atgtcgcgca atgacgcacc
     1261 cgagaacccc ggacccgcct gcgacgcccc gggatacgtg gtcaacccgt cgatatcggc
     1321 aagggtgagc ccggcgtcct cgacagctgc gacgcacgca tcggcggtga gctccaacgc
     1381 atcacgcccc agccgtctgc caatgtctga ctggccgatg ccactgatga tcaccttgtc
     1441 ggtgagcagc gacgtcatca atgatccggc tcgaacagtg ggatgaacac ttcgtcctca
     1501 ccatcgacgc accgctgaaa ggtcactcgg accggcatgc ctgcagtgac gtcatcgggt
     1561 tcgacgttca ccagattggt caacacccga acgcgatcgt cctcgacagg cgtgacgaac
     1621 gcgatcacat aggggggtgt gaatcctgga aagaactggt gctggttgac ggtccacgtt
     1681 tcgactattc cgcgaccgct gagcgccacg tattcaggca ctccgtggtc atgcgggcac
     1741 cgcaatgccg gagggtgcac aagccgctcg cagtcagtgc accgctgcag gcgccattca
     1801 ccattactgc caccagtcca atacggtcgg ttcagatccg tgagaagcgg gactggccgc
     1861 tcagccgcga tctcgctcac tgctcatcag cgccgaagat gaagcgatcg gcgatcgcat
     1921 gctcatttcg gatcgacatc tcctgagcgg tgttcagcca cagtccgtta aaacccttgg
     1981 aatgcatacc ggcatggatg gcgccgatgt tcttgagatc ctgcgtcggc agctctcccc
     2041 agccgtcgtg gtcttgccag cgctcgaact cctgccagct ggtttcggga acctcgccgg
     2101 gcgcgaaatg ttcgagggag aacatctcga aggtgcaccg gttgggatca tcggcgtcgg
     2161 gaagcatccg atagcccagc agacaagact tttcgacgag aatcaccagc gtcgggaaga
     2221 cgtgccagtc gccattgccc gcgaagtact cctccatcga catcttcgga aagtcgacgc
     2281 cgtcggcgcg cgccaactct tcacacatct tgtgatacag gacaaacggc ggaacctcac
     2341 tcggcttcat cgtcgctagc tgctgtgcgg cgcgatagtc gcgctcggtg accagcgagc
     2401 cgacctcgag gtagttgtat tgcatcgagt tggcgaacac ctcaggccgg gcggcctgct
     2461 gctggcgggc gcggtcccgg ccggccgact cgggacgttg cgtgtagatg aatcgtgagt
     2521 ggttgtcata ggtaatcgtc ggtgtgtacg gagcgtaggc gtattcctgc ggcaatgccg
     2581 gttccgtcgt cggccggggg ccctgggcgg ctcgcagggt ctgagggtgc gtacccgggg
     2641 tgtggtagcc ctcgatgaac gcatcgacaa cggttttgta gttggcaggc aggaacgttc
     2701 gcttgcgcca ccggtagcgc atgtcctgca gccggaacgg cgccagcgct gtgggtagag
     2761 gagcaagcca gtccagaaga tccggtggat catcggccat gctgatgaat acccaaccgc
     2821 cccaggtgcc gacgttgatc ggagtcagtc cccactgctc ccgcgggcgc gaggcgaact
     2881 cttcgcggca cggaacgaac gacgatcgtc cgtcgagcgc atagcgccag ccatgaaact
     2941 cacaacgcag gtcgccatca accgcgcaac cactaccgcg cacgaccttc attcctcgat
     3001 gcgcgcatgt gttgtagaac gccttgatgc tgtgatcctt ctgacgcacc acggcaattg
     3061 actgccggcc gattgagtac tcgtagtaac aacccgcgtc agggatctct tcttcacgac
     3121 acgcgacctg ccacacttgc gtaaagagtc gatcgagttc gagtcggaga aagtcagggt
     3181 cgatgtaacg gctcttgggg acgaaggtct caccgcacgc gtacttctcc ggcacccgtt
     3241 cggtagccag gtcggtcatc gtcacctcat ccgtaggtca gctcgcaggc ggcttgtcga
     3301 agatgttggg ccacaacacc gcttccttga acgatgtctc gatctcggcg aatgcggtgg
     3361 gaatgtccca cgtgccggtg gccgatacga tctcgcgctc gacgacgggg ttagacatca
     3421 agctgattcg gccgtttccg gcgaagatga ctcgtcgatt gagccagtcc gactgggtac
     3481 tcgccagata tgcgaccggc ggcacacagt tcttcggcga gaggcgcacg ttggagtcgg
     3541 agtagtccat gcttccgccg cccttgatgc cttgcgtcat tcgagtgccc gcgatcggtg
     3601 cgatcgcgtt ggacgtcacc ccgtagttgc gcaatgcgtt ggcgcacgag aacgtcaacc
     3661 cgacaatgcc catcttggcc gccgcgtagt tgggctggct cggcgctcct tgcaaacccg
     3721 acatcgaggt gaagttgatc agcctgtact gaccgccacg gttctcgcgc caccacccag
     3781 cggcatgtcg ggtgatattg aaggtgccct tgagatggac ggcgacgaca gcgtcccaat
     3841 cctgctcact catcttgaag accatgccgt cgcgcaggat gcccgctgcg ttcaccacga
     3901 cgtccaggcc accaagtgtg ttcgcggcgc gatcgatcag atcggcgcag gcggcgaagt
     3961 cggtgatgtc ggtcgcgtct gggaacgcct tgccccctcg ttcattgatg gcagcgacga
     4021 cggcttctgc cgggccggca tcgtgacctg tgccatcgac gccgaccccg gcatccgaaa
     4081 ccaggacggt cgcgccttcg gccgccaggc cttcggcgat cgccgccccg ataccccggc
     4141 cggcgccggt gaccagcgcg gtacgtccgt ccagtgcacc catatgtcac tcctagtcga
     4201 agaatctacc gcccagcata cagaacatca tgacacaatt tgttcaggtg ttacacctac
     4261 tcgaatcccc actccgagta gcggcgctcg agttcggcca atgcctcacg gccccctcgg
     4321 gcggcgcgtt ccttcatgaa gttgaactcg tcctcgcgcc atgacaagtt gctgaacacg
     4381 ctgtgcgcca tgggaaccca gtcgccgaac tgcgccatgc cgaccgcgtg ccagaacgcg
     4441 ataagggcgt gcttgccaac catcagtccg tcggccgagt gatgagcgat ggcgcgcgcg
     4501 tagcgcagtg cctcatcatc cagtccgcca accggcacga cggatgcggc gacgccccag
     4561 tcgcataatt cgctcgcgga gacctttcgg ccggtgatca tcgcctcgta accacggttc
     4621 ggaccaagtt tgagcagggc caatggcatc gccgtcgaga atcccgcgaa gccgattcga
     4681 gcctgcggcc gcgccagata catgtcctcg gagacgacca agatgtcggc ggccagcgcg
     4741 agattcatcc ccgctcccag cgtggcgcct tggcacgcgg caatcaccgt cttgggaaat
     4801 tcgagccagg tcgtcagatg gcggtgcagt cgacgtgcat tggccatgcg agccgtttgc
     4861 ggcagccttt tatcctttgt cagcccagcc tgttcaaccg gaagacgccg gacgtcgtct
     4921 ccgctgcaga agtcttttcc ttccgccttc aggatgacga ccttgatgtc atcgtcttct
     4981 tcagctcggt cgaggcgatc cctgaacagt gcatgcatat cgggcgacag gatcgcgttg
     5041 cggcgttcgg gtcgattgat agtgatctga gcgatgtgcg gttcgatcac ggcgtaacga
     5101 acccagtcct catcgaccgc gagagccgtc tggtcgtcag tgctcatgtc acctcaattc
     5161 cgcgctcggc cctacagcac atatgaacac tatccgctct attgttacat gagtggtact
     5221 gtcccccgca tgccaaccat gactgtcagc aaggacatct ccgcgcgagc tgacgctgtc
     5281 tgggcgatac tcgccaactt cggtgaggtc ggatggatcc cgatggacat caacgtggag
     5341 gtcgaaggcg acggtccagg gatgcgcagg aagatccagg gttcgggcga cgatgcgacg
     5401 gtggagacgc ttgtctggat ccgacccgag cagaagagat tggcctacga gatcaccaac
     5461 aacccgctgc cggtgaaccg cttcgagact gtggtcttcg tcgccgattc tgctgtcgcc
     5521 ggtgatgggt gtcggatcgg atgggaagtg gactacgaac cgtccggtga cgatgccagc
     5581 gcccgggaga gcatcgagct cgtttacggg atgatggccg gctggatcga agacgccgcg
     5641 acaagtgccg gcgcctagtg tacacatata cagattttgt tcattagtct agaatgttgt
     5701 cggtgatcga tcccctcgac ctagtgctga tcagcgtcga cgaccacacc gtcgagcctc
     5761 ccgacatgtt caagggccga gtgccgtcgc ggttcgccga cgatgcgccg aaaatcgtgc
     5821 aaggcaacga cggcgtgtgt ttctgggagt acgcaggact ccagatgccc aatatcgggc
     5881 tgaatgcggt tgcgggccgg cccaacgacg agtggggatt tgaaccgtcg cgcttcgagg
     5941 acatgcggac aggctgctac aacgtcgacg cacgagtcga tgacatgaac gcatctgggg
     6001 tgctggcgtc cctgtgcttc ccctcatttc ccactatctc gggggcgctc ttcacctatc
     6061 acggtgaccg cgacatatcc gaagtcatgg ttcgcgccta caacgactgg cacgttgagg
     6121 actggtgcgg ccgccacccg gaccggttca tcccgatggg catcctgccg ttgtgggatc
     6181 cagttctcgc cgcgggcgag gtggcacgcc tggccggagc gggctgtcat gcggtgaccg
     6241 tgccgcagca catcggcagc tatggtcagc cgccatggca ggatccgcag tgggatgtgt
     6301 tgtgggagtc ggtctgcgag cacgacacgg tcgtcaacat ccacatcggt accggcggcg
     6361 gggttcccgt cccgtcagat cagaccacct atctcgcgta caactcgatg ctggccattg
     6421 acacgatgcg gttcactgcg gatcttctgt tctcccccgt cgtcgtgaag tttccgacga
     6481 tcaagttcgc gctctccgaa ggcggaatcg ggtggattcc gttcctgctc gagcggttcg
     6541 aggacatcta ctcccgccag cgcgcgtgga cgggcgacga cctcgggtcg ggactgaccc
     6601 cgaccgatgt cttccgccgg aacttcatgt cgtgcttcat ccgcgatcgg gtgggaatcg
     6661 agatgcgcga ccgcataggg gtcgaaacca tctgctggga gatggattat ccacattccg
     6721 atagcagctg gccggatgcc cctgagcagc tcgccgccca gctggaggga tgttcgccag
     6781 atgaggtcga agccatcacc tggcgtaatg ccgcgcgcgc cttcggctat gacgctgtcg
     6841 agcgtcttgg acgagagaac tgcaccgtgg cggcacttcg ggacaggatc gtcggaatgg
     6901 acctttctgc gccgaaagtc gacgcaggcc gtgcacccaa ggccggtacc accgcgatca
     6961 cctacggcga gatgaaagag cggatggcgt cgatcattcg cggcggcacg tgaccgaccg
     7021 cgactacggc gttgtcctca acgacgcgga cgaagcgttc cgcgacgagg ttcagtcttt
     7081 cttatcctcg tcgctgaccc ccgacttggt cagccgagaa ggcgaagaaa ccgatcacct
     7141 tgatcgaatg cagctgtggc agaagcgcct tcacgctgct ggcctggccg cgatttcctg
     7201 gccagtcgcg tacggcggac gcgccgcgac accgacccaa cagctcatct tcaacgccga
     7261 gatggctgca gcacatgccc cggagccgat caaccgcagt gccatcaatc agctgggccc
     7321 cacgatcatc cagtggggca ccgacgagca acgtgcgcta tatcttcccc gcatcctgtc
     7381 tgctgaagat gtttggtgcc aaggcttttc cgaacccgag gccggcagcg acctcgccgc
     7441 gctgaaaacc aaggctgtga tcgacggcga cgaactcgtc atcaccggac agaagatctg
     7501 gacgtcgaaa gcctcatacg cagactggat ctacattttg gctcgtaccg actcgaccgc
     7561 cgcaaagcga gacgggatca gctacatact ggccgacctg aattcacccg gtatcgaagt
     7621 ccgacccatc cgtcagatca caggtgccgc ggagttcaac gaagtgtact tcgatgctgt
     7681 gcgagtaccc ctgagtaatg ttctcgggcc cctacacggc ggttggaaag tggctaagtc
     7741 gacccttggg tacgagcggg tcggccaaag ccgtacgcat cggatcgaac gccgcctcga
     7801 catcttggtg aagatggcgc aacaaccaaa tgcattgact gacaaggggt tagctgatag
     7861 ctatgtcgcc gatcgaatcg cccgatacgc ggcccaagtg gaggcgttga ggcagatcgc
     7921 cgcgcaggcc acagcggcgg gggttcgcgg agtaagccca ggcccagagg cgtccgtcgc
     7981 caagctgctg acctcagagg ttgatcagtc gatggcaaca ttcggcctcg aactcgcggg
     8041 gcctgcggga acactcgaac gcggatcggc aggcgccgcc aagaaaggga atgttgctca
     8101 aagctacctg ctgatgcgcg cagcaacatt cggtgccggc acgtcggaga tccaacgcaa
     8161 cgtcattgcc gaacgattac tgggattgcc ccgtgacccc tgacaccgca atctcgccga
     8221 cagtttcact ggacgaaatc gcagacactg cagctgaact cgccgaactg cgccgatcag
     8281 ccgacgatat tctgcggcag gcctggtccg ttgatcgcag ccgagcgctt ctggacggac
     8341 ccgggccggc attcgacgaa gcactatgga ccactgtcac ggatctgggc tggccggatg
     8401 tattggtgtc ggaagacaat ggaggcgcag gtggagcact gcgcgaactc gctgtgctcg
     8461 tcgaagcagc cggtgccgca gcagcgccga tacctctggc cgccaccgca gccgcgggct
     8521 ggtccgagga ccggtgctcc gacggggtcg cagtgcttct gcctgagtcg ggaacacttg
     8581 caggccagat ggtttcgggc accttcaatt ttgttcccta cggcgccgtc gcaacgcggc
     8641 tggtggtctt ggccgaagac aaccgacagt cacttctggg tgtggtggat ccgaacggaa
     8701 tgggcgtcaa acgggcgccg ctgaagcctc tcgatcacgg ccctgcggcg ctgatcaccc
     8761 ttgacggggc accgctccga gtgatcaccg acggcgccga cgctgtgcgc cgccatcgag
     8821 atgccctact gcgcttgcgc gtcggactcg tcgccgaact ggtcggcgtc gcggcagccg
     8881 ccaatgacgc agcaaccgag tacgcgaagg ttcgcgtcgc gttcgaccgg ccgattggct
     8941 cgtttcaggc gatcaagcac agactggtcg atcagcgttc ggcgatcgaa gtcggccgcg
     9001 cgctcgtcaa ccgggccgcc gatgcgtgcg atcagcacca ccccgacgca acggcgttgg
     9061 tgtcgctcgc cgctttctgg gccatcgact cgctacgcgc cgttccggaa ggggcgacac
     9121 aggttttcgg cggcatcgcc tacacctggg agcatgaggc tcatgtgcat ctgcgccgag
     9181 ccgcaaccct ctccgcgtcg ctggggcccc gtgcagagca tcgcacggcg atcaccgact
     9241 ggctggctgc caggagcggc gttcagcgat agtccagtgt gacgtcgagt cgatcgaacg
     9301 tgttcaccag cgacgattcc cgtttgatcc gctgaccggt gacgtgaact gccttggccc
     9361 gtcgaaggac ctcggcaagc acgaaccggg cctcgcgccg cgcgagggcc gcccccatgc
     9421 agaaatgttc tcccacgccg aaacccacgt gtccagccgc gtcggagcgg gtgatatcga
     9481 agtcctcggc agtgggtccc caatgatcga cgtcacggtt ggccgacgca tacgccatca
     9541 gaagaccctg accttcaagg actttctttc cccgcaactc gacatcgtgg gcggcctgcc
     9601 gggccatgct catcaccggt gtccaccaac gcaatgtctc ctcgacggtg ttgtcggaca
     9661 gcgaagagtc ggcgaacaac gcgtcggccg cagcgggatg cacatccaaa cactcgatga
     9721 gacctgccag cagactctga gtggtctcgc ttcctgctgc gagcagcgtc atcgcgtaga
     9781 ccatgatctg cacctcgtta aacggctcat cgtcgatgcg aacctgcgtc agcatcgtca
     9841 ggaggtcgtc ccgggggctc tcggcccgtt cggcagccag acccaataag tatggcgcga
     9901 tctggtcgaa gatcacctgg aagtcctcat cgctcgccag cccgctgccc gccttcgcga
     9961 tagtggtggc ccacgacgcg accttggacc agtccgactc cggcacaccc aggaagtacg
    10021 aaaagatgta caccggaatc ggttcggcga tgtgctcgat ccagtcgaac tcgccgtcgg
    10081 gaaggttgtc gaatgcctgg gcgacgacct gcttgacgcg gtcttccatc ttggcgaccg
    10141 ccgccggtgt gaaccgaacg cctatcgctt ttcggtgcgc gcggtgttca ggcggatcca
    10201 tgaacatgat tccgcgactt ccgaatccct gcgggtcgac accctgtcgg tgcgcaatca
    10261 gatccatcat gatcgcgatt cgctccgacg tgaacttctc cggccgcgcc gagatcgtct
    10321 tgatgtcgtc gtacttggtg acgacccaga acttgccttc ctcgtaccag tgcagcggat
    10381 ccgtctcccg caactcacgg aacgtcgcgt tgggatcgcc gagataaaac cctgggtcgt
    10441 cgaatcgggc ttcggaaatg ccggtgatcg tcgccatagc atgaacaata gaacaaaaac
    10501 tgtgaagctg tgaaccgaac tcccgatgcg acgtcgccga cccggtgtgg actcaatgag
    10561 acgaatactg ttcaattgtt caacacccct gtgaacagga gcgcatgcag tgagccacaa
    10621 tccttccacg acggatttcc tcgctgacgt gcgcgtgtta caactcggcg acggaatcgc
    10681 cggctcggcc gccgctgcgc tgctggcgag ccttggcgca gacgttgcca agatctcagg
    10741 cgagaaggtg cgccgggcgg gtccggttct gacggggccg cagggcataa cgccggccgt
    10801 cgacttcgtc cttgaccatc gcaagcgcat gctcgatccg ggtgccgatc ctgtgcagca
    10861 cgcgcgcgca gccgacatcg tcatcgtcga cggcgctgcg gttccatcgg tggatggccc
    10921 cactgtcgtt gttaccctgt cccctttcgg ctctgacgag ccgggtaccg gacacccggg
    10981 cggtgagctg gtggctcagg ccagcggcgg actcctggcc acaatcgagg acggcgacgg
    11041 tcggccggtt cccgcgcccg gctatgtcgc cctcaagtcc gccggtgctg tcgccgcgct
    11101 ggcagcgctc cacggactcg accggcgaaa cgacacactt ttaccggttc acgtcgacgt
    11161 gtccgtgcag gaagcagtga tctacaccgc cgcactccca gaatgcgcgc acatcatgta
    11221 ccgctgcccg ggccgcgcag gcagcggtcg atacctggcg ccgtcgggag tgttcgcatg
    11281 ccgagacggg ctggtgcgta tcaccgccgt ggagaatcac cagtggaaag gcctgttggt
    11341 cgcactcggg aacccggcct gggcggtggg ccttgatgat cgcccggccc gcatcgagca
    11401 cgccgaactg atcaacgcca aagtcaccga gtggaccacc ggtcgcgcaa aaggtgattg
    11461 cgctgccctg ctgcaagcca acggcgtccc gtcgacgcca gtcaatctcc cgggtgagct
    11521 cttgagctcg ccgcaactgg cccatcgaag cgcattgacg aaggcaccga tgggtgatcg
    11581 ccacgtcgtg gtcctcggaa ctccgtggcg gacagagcta ggcaacgccg gtcaacgccg
    11641 acgcggcggt ctgcgggggc tgcgcatcgc tgagctcacg cacgtacttg ccggcccgat
    11701 catcggttcc ctattggggg cgatgggcgc gacagtggtg cggctagagg atcccgaacg
    11761 gctcgacatc taccggcgca gtggcccgtt cgccgacgga gtagcaggtg tcgaacgcgg
    11821 cgcgtactac gcggtcgcca accactccaa acgaagtgtt ctgatcgatc ccggcggcgc
    11881 agtcgaagac gtggccgcgg ccctgtcgga agccgatgtg ttgatcgaga acgtcgggtc
    11941 gtcccggttg gaccggctgc acgtctcacc cgcggaactc gccgaatcgg ggcgacttgc
    12001 ggtgcgggtg tcaggcttcg ggtcggacgg tccactggct ggatatcgcg tgtacgccaa
    12061 caacgttcag gcatacggcg gtctggccgg cttgaccacc ggacccgacg gcgagcccgc
    12121 tcgcctgggt accgtgctcg cagatccgct gtcatctgtg gtggccgcgg tcgtcatcgc
    12181 ggcgtgggcg cttggcccgc acagcgacac aggtgctgtc gtggacgtct cgatgtccga
    12241 ggttgtcgca tcgacgattt cggagtacgt cgccgcggct tcggtcgacc aagctgctcc
    12301 cgacccgacg gtgcgtcgcc tcgtgtaccg cagcgccgac tcgcgctggg tcgcggtcga
    12361 gttggtcggc gacgaatcgc gaccgtacaa cgatggtgag ctcgcggatc ggatcgcagc
    12421 agccgatgcc gaggacgtcg cggcacaact cgcgggtgcc ggcgtacggg ccgctgtcgt
    12481 acgaagtgcc ggggaagtgg taaccgacga gcgtctggcg gcgcgtggat tctttcccga
    12541 gatcacccac cccgacccgg atctcggcac agctcgactg gtgggcctac cgtggcggtt
    12601 tgcaggtgag ggcccggtgc cgctcgcacc accgcccgcg ctgggcagca cgagcaccag
    12661 cgccctgtac gagaggacgg ctgtctgaca atgtcaggat tccaaacccc ggcgaccgta
    12721 tggtcgtcga ccttcgttcc gtcgaagtca cccttccctg agttcggcca ggatggctat
    12781 gccgtggcgt gggtggacac cgaggacgga cggttccagg ttcttgtcga cggtgcccgg
    12841 cctgtgccgg gaaccgttgg tcgactcgtc tcagcgacat tgggtgaaga cgccatcgag
    12901 atgtttgtcg cggatccgac atgacgggcg tacacatcac cggagtcggc ctcaccgggt
    12961 tcggcaagca cagcgatacg cccctggctg acctcggcgt cgccgcggcc gagaacgcgc
    13021 tggccgacgc gggcatcggc tacgacgccg tcggcgaagt gttcacctcg tcggcactgg
    13081 cggggccgca gaccggcctc gcggtcgcac atcggttggg ccgcacagga attccggtaa
    13141 ccgcgacgga gagcgcgtcg gctggcggaa tggtcgcact gcgacacgct gtgtgggcgg
    13201 tcgcgtccgg ccgctgcagc acggcgctgg cgatcggcta cgagaagacc accgcgttgg
    13261 agccgggcgg agtggtccct gcggccgtcg gtttctggga ccacttcccg ccgcagttgc
    13321 attacgccat cgaggcagcg cactggctgt acgaaaccca gtgcggaccg gaggtcatcg
    13381 ccgcggttgc ggccaagtca tacaaccagg cccggctcaa tcctcttgcc gcgcgacgca
    13441 acccagagcg ggtcacggtt gacgatgtcc tcggtgcccg ggtcgtcgcc gaaccactga
    13501 cgaagatgat gtgccacgca tcggtcgacg gcggggcggc ggtcgtggtc tccgccgaca
    13561 cggcaccgcg ttcggtgtca gtgctatcca tcgagcagac cagctggccg aaagatccag
    13621 ggtggccgtt ggtgggaccc gttgtggggc cgccctctca gataacgctg actgcccagc
    13681 gcgcgtatgc caatgcggga gtggggccgc aagacgtcgg agtggtttcg ttgcacgata
    13741 tgtgtgccag cgaagagatc accgcgttga tcgcgatggg cctaacggac gcggacggag
    13801 tcgtgaagct tgccgagtct ggtggactgg catcggacgg ttcgctgccg accaacaccg
    13861 acggcggatg cattgcgcgt ggccacccca tcggtgcaac gggtttggcg cagaccgccg
    13921 aggtcgtcag tcaacttcgt ggagaagctg tttcgcgtca ggtcgccgaa ccgcggaccg
    13981 gcgtcgtaca ggcagtgggt ggcggcggct cctgcgtcgt ggcgctcctg cgcgggtgac
    14041 gttcaggcga tcgcaccgct ttccttcagc gccatgatcc ggtcccagtc gccgccgagt
    14101 tcgagaatca gttcctcggt gctggccgcg aattccggca tcgggctcaa gtgcagtggc
    14161 gtctcgtcga acagcaccgg actcgcgacg atttcgaagc tctcgctttc tgtttcgatg
    14221 ccgacgatgc cgccgttggc gcggacctgt gggtcagcgg ccacctcgac cgtgttctgc
    14281 acgaccgacc attgaccatc gaagccgtcc aacgcttttc gccaatgctc caaggggtga
    14341 gctgcgatgg cctggcgcaa gacctcggta gccgcaggcg cattgcgcgc gagcaactcg
    14401 tggcttgcga aacgttcgtc ggcagcgagt tcacccacac cgatgcgtcg gcagaactca
    14461 ccgaagaagc gaaagccctg cagcatggac aagcccagaa atcggcggtc gctcgtcccg
    14521 tagaaaccga ccaacggatt gttgggggca acgttggctc ctgcagggtt cgctacccag
    14581 ggttgtccgc tgaccagcgc cgcgttgatc gccactcccg acgcccatac gccgacgcct
    14641 agtagtgaga tgtcgacgat acgggcgtgc ccagtgcgtt ctcgctcgaa cagcgccgcc
    14701 gatatgccgc cggcaatcgt catccccccg atcgaatcgc cgtatgccgg accaggttgt
    14761 ggtacaacgc cttcgaggtc gctcggtgtt gaccccaagg cgcctgctgc gcgactccaa
    14821 aaaccggtca tgtcgtagcc acccgtaccg gcctcggccc cgagggggcc gtacgcgctg
    14881 ccccgggcat agatgatttt tggattgacc gcacgcacat cgtcgacgtc gatccggagc
    14941 ttctcacggg cgtcaggtag aaagttggtc aggaacacat cgctggtgcg aacgatgtcc
    15001 atcaacagtt cgtggccatc tggggtcgac atgtccagcg ccaccgaccg tttaccgcgg
    15061 ttggcgtgtt ccatcaccgg attgcggttg ccctcgatcg tgatgtgacc gagattgcgc
    15121 aggccgcgct gggaatcgcc cgtccgtggg tgttcgatct tgatgacgtc ggcaccccag
    15181 tcggccagca cggcgcccgc agcggggacg aatgtccact gcgccacttc cagcacgcgg
    15241 acgcccttca tcggtttggg ttgcgtcacg aactgaacaa tagaacactt tctgtctagc
    15301 tgctattgtc ggatccgtga ccggcgcgtt gtcccacctt cgggtgtgcg atctgagtgg
    15361 ccagctcgca ggcgcgggag ccaccaagat cctggctgca ttcggcgccg aggtgatcag
    15421 ggtcgaagat cccgcgaccc aggggctgtg ggacgcgttg cggggggtcg gcccctacgt
    15481 cgacgatcgt cgcggggtca acttcggcgc cggattcaac aaccacaacg tcggcaagta
    15541 cggcgtgaca atcaatctgc gcaccccgga aggcaaagag ctactgcgcg agttgatctc
    15601 cgtctctgac gtcgtctgcg aaaatttcgc ggccggggtc atggagaacc gaggcttcgg
    15661 ttatgccgag ctgcaacgga tcaagcgcga catcatctat gtctccaact gcgggttcgg
    15721 ccatacgggt ccctaccgag acttcaagac ctggggcccc attgtccagg ccgtgagcgg
    15781 gctcacgtat acggctggac tcccggacac cgagccgtcc ggttggggct tttcctatat
    15841 ggaccacggc tcggcgttct acatgacgac cgcgatcttg gccgcgctgc accaccgaga
    15901 caagaccggt gaaggccaac acgtcgatct ggcgacggtg tcggccggca tcgcgatgct
    15961 gccgacggaa atactcgatt ggacggtcaa tgggcgctcg acggcagccg gcggcaaccg
    16021 agccgacttc ggcgagatgg cgccccacgg catatacccc tgtctcggcg acgaccgctg
    16081 gatcgcgatg gcgtgccggg acgagcgaga gctcgccctg ctcgcgaagg tgctcaacga
    16141 acccgaactg gcgtcggatc ggttctccac cctcgggcag cgcttgcagg ccgtcgacga
    16201 gctggaccag atgatcagcg ccgttacgac gacgcgggac gctgcggccc tcgccgagga
    16261 cctgaatgcc gcaggcgttc ccgcaagcgt cgtcaaaagc cccgccgaac gcatcgacgg
    16321 cgatccggat ctcgccgcga tggagctgtt cccgacggtg acgcatcccg acatcggcga
    16381 tgtccgcgtc gagggaatcc cgatgcggtt ctccgcgacc ccatggcaga tcacttctgc
    16441 tgcgccgcgc gtagcgcaac acaaccgtga agtcctgaag gacttactcg ggcactccga
    16501 cgccgaactc gacgactgga cacggcgagg tgtcatctga gcgcactgca agatctccgc
    16561 gtcatcgaga tcgccgacca gttcaccccg gtcggcggcc gagtgttggc cgagctgggc
    16621 gccgaggtca tcgtggtcga gccgccgtct ggctcaccac atcggtccag gccgccgttc
    16681 gtcgatgacg agcccggtat cgaccgcagc ctgcgctggt gggcgggcaa cgtcgggaaa
    16741 cgctcggtca caatcgatct gacgactgaa gcaggcgcgc gcaccctaga agacctaatt
    16801 gcgacagccg atatcgtcat cgcgggcagc gaccagtgcg agggcacaca catcgactat
    16861 cagcgttgcg cggcccgtga tggacgtctg atctggatat cagtgagccc gttcggtttg
    16921 cgcagtgccc gcgccgatca accggtgacc gacctcacaa tgctggctgg cggaggcccg
    16981 gtctggaact gtggatacga cgatcgcacg cttccgccga tccgcggcgc cggcgatcag
    17041 tcggccaata tcgccggaat gtactgcgcc atcgggacgc tcgtggcgct ctcacatcgt
    17101 gatcacaccg agcagggcca actggtagat gtcaatgtca ccgccgcctg caacgtgacg
    17161 tgcgaacaga cgacatatca ctggctggtg aaccgggacg tctgtctgcg tcagaccggc
    17221 cgacacgcgt atccgacccc cagctcgaaa gttcaagtac gctgcgccga cggtcattac
    17281 gcaacgaccg gtgtgcttcc ccgcaagccg gaggagttcg cgcgcctatc atcctggctt
    17341 ggcgaactcg gcctcactga agagctgccg gaggcggtgt tccttgatct cgctgccgaa
    17401 cgcgatgaac cagtcgatct tgcgttgatt gggacagatg acgagaccac ggcgatcctg
    17461 agcgcggccc gcgaggcaat tcggctcatc ggttcacggc ttcccgccaa ggagttcttc
    17521 gtgaccagtc agcgtcgcgg gtttccctgt ggcgctgtgc tttcgcctga cgaggccttc
    17581 gaggacgagc acaccgtggc gaggggcttt ccggtgccag tggcgcatac agaattgggc
    17641 aggacggtga cctacccggg cactccgtat gtgttcagcg cgaccccaac tgtgacgccg
    17701 acccggccgc cgctgctcgg cgagcacaac gacctgattg ccgagctcgt tgcgcagtgg
    17761 ccatgaacaa cgggcatcgc ctcacccact tcggcttgtg cgttcgagac atcggccgtt
    17821 ctacggagtt ctactgcgct gcactgggtt tcaccgagat cgggcgcatg agagtggacg
    17881 acgatgccag cgcacggctg ctggatgttc ctgaccttgt gctggacctc gtctacctgc
    17941 atcgcgatgg attccgcctc gagctgctcg gatacgccag acctgggacc atcggagaac
    18001 cggcgccaga gccgatgaac cgactcggct tcacccattt gtcttttcgc gtcgatgacg
    18061 tcgacggcat gatcgcgacg atcgtcgaac acggtggccg ggcgctcgcg gaccgcaccg
    18121 tcaagttcga tgggggtaat cgcggcgtga tggcgacgga tccagacggc aatctactcg
    18181 agttcatcga acgcattcca cggctgacag gcagttgaga aatggtccca gcgacaaacg
    18241 aattgatgta ttcccccttc tcaaagacca tcttcgacga cccgtacccg gtataccgca
    18301 ggctacgtga cgaagcgccg gtctatcgcg atccagaaga ccggtggtgg gtgctgtccc
    18361 ggttcgacga tgttgccgga gccttgcggg actggccgac gtactcgtcc aaactgggtc
    18421 ctgctccgga aaacccggac gacgacgggc gcaagtattc cgtcatctca atggacccac
    18481 cacgccacga ccggatccgc ggcgtgctca aggggttctt cacaccaaag gcggtcgccg
    18541 ccatggagga cgccctgcag accgtcgtca cccatcactt ggaccgattg cggccgggca
    18601 ccacggtcga tgcaatggaa gcgttcgcat tctcggtgcc cacggacgtc atcggcgacc
    18661 ttcttggagt tccgaaggcc gaccgcgaac aactaagagt ctggtgggag gcctttctca
    18721 cgcgccacga gggcgaggtc gcgatgccgc ccagcgccat tgaggcaaac cgcaagatca
    18781 gtgcctacat cggagatctc atcgaggaac ggcgctccaa tccaggcgag gatctcatca
    18841 gcattgtgtt gagggctacc tttgacgacc ccgaggtcgg cggcgaacgc tcactcactc
    18901 cgcacgaggt attgatgttc gccaacctgc tgtcggcggc cggctcggaa accacgcaga
    18961 agttgatctc caatggcctt gttgccctgc acgaccatcc tgaccagtgg cagcgaatcg
    19021 tcaacgacca cagcctgatt cctgcagcag tcaacgaagc actgcgatac gacacaccca
    19081 gccactgggt tgcgcgcacg ctgacacggc cagtcgagaa gcatggagtg acaatgaacg
    19141 ccggcgactg ggtgctgctg ttgctcggca gtgccaatcg cgacgagcgt cgctacgaca
    19201 gtcctgaccg cttcatcatc aaccgtccgc gcgggaccga cgtctacttc ggctggggta
    19261 tccacatctg tctcggtcaa tggctggcgc gcagggaggc acagcttgtg ttcgagtacg
    19321 tcgccaagaa gttcccgaac tacaccgttg gtgcgcacga acgcgtcctg acagccaccg
    19381 tgcgcggcta caccagcgtg gaaatgactc tcagctaaga gttgaagacg ccaggcgact
    19441 gcgagtagtc caccgttgac aactcggtca tcccgccgtc gacgtggagc accgtaccgg
    19501 tgacgaaaga tgcatcgtcg ctcgccaaga aggcaactgc gggcgcgatt tcggagtccg
    19561 gatctgcacc gcgcttcatc ggcgatgccg tcgccgtgcg cgtgtagttg tcccactcgg
    19621 tgccgatcat gtcgcgctga acatcggtca gcgcgaatgg acacaccgcg ttgacccgga
    19681 tgccgtggcg cgcccactcg ttcgcggcga ccttggtcaa ggcgtgcacg gcccccttgg
    19741 cggcggaata cggtccggtg tacgccatac cgccgagcgc ggaacccgat acgaagttga
    19801 tcaccgacgc cttaccggtt ttcttcaaat atccgaacgc gctctgcatg aaccgaaacg
    19861 tcgcctttgg acccgttgcc tccgatagct cccagtcctt gtccgtcacc agttctaggg
    19921 ctttcggcat ggcgaagtac tgcgccgcgt tgaccaggat gtccaaccgc ccgtcgaccg
    19981 cggccgccgc ggtggccgcc tcgatgtcgt cgcgcttagc cacattggcc ttcacgaact
    20041 cggcggtacc ccctgcggat tggatcagcc cgatgcactc acgacccttg tcctcgttca
    20101 agtccacgat cgtcacccgc gcaccctcgg cagcgagtcg acgcgcaatc gcccgcccca
    20161 acccctgcgc gccgccagtg accagggcgt ttcgatcatc caagcgggac aaagtcgagc
    20221 ctcctagatt gaacagttga acattattcg ttctatcatc caagatggta tcggaacgta
    20281 aaggagaggg ccgatgcgcg tcgaagtgga tcgcgatcta tgcgagtcca acgcggtatg
    20341 tgtgggtatc gcacctgaca tcttcgatct cggagacgac gatctggctc gcgtcttggt
    20401 cgacgaggta ccgcagggcc gcgaggacga ggtccgcgaa gcggtcggac tgtgtccgaa
    20461 gatcgcactg aagctgcacg aagactgacc gcttccgatg aacattggcc tcaccgacga
    20521 gcagaagttg ctgcaggaca ccgcatccgg actggcggag aatctggcga ccacggatcc
    20581 gagcgacatt ccgagtggcg acacactggc ggaccactgg cgccagctcg tcgagctcgg
    20641 tgtccccacg ttgcgctccc ccagcctctg cggcctggat gcgagtggcg tagagacggt
    20701 cgtcgtcatc gaacagctgg cccggacaat gtgcgctgtg ccggttgtcg gtcaggcagt
    20761 cttcgccacc gagttgctgg aagctgcggg ggcgcacaag gagctggaac tcattgccga
    20821 gggcagcttg cgcatcgccc cgatacttac tgttgacttg cagagatttg gggcgacgga
    20881 agccggcgga gtcgccttcg atgccgcagg cgcgacacac gccctcctcg cgatcgagtc
    20941 gggcggcgtc catcggctgg cctgcgcacc gctcgaagac accaccgagg agggattgga
    21001 cctcactcgt accctgcgac ggacgccggc cgatgttgcg gcgtcggcaa cgaccacagg
    21061 agaaccgatc gatgcggacc gctggcatcg agtagaggct ctcgcattga ctgcgacggc
    21121 ggcggacttg gtgggtgtca tgcagggcgc attggatgag gccgtgaggt acgccgggga
    21181 tcgcgtccaa ttcggcgtca aaatcggttc gttccaagcg gttcagcaca tgcttgccga
    21241 tgccctcgta cgcgtcgaag gcgccaggag ttgcttgtgg cacgccgcct gggcgatcga
    21301 ccatctccca gtcgatgagg cccgactggc tgcgatgact gcgaaagcct acgccgccgc
    21361 cgcgggccga gatgtcgtcg agacggcagt ccaggtattc ggcggaatcg cgatcacgtg
    21421 ggaacacgta tcccacctac gggtccgccg aaccctgttg aatcgacgcc tgttcggtga
    21481 cgaggccacg ctttacgaag ccgtcgcgca gatgcggttg gagaacgagg agttggcgta
    21541 gccgatggac ttcaatgaca ccgccgagga agccgaattc cgcgcgcgcc tacgccagtg
    21601 gctgcacgac catagcgccg atgccgccat acctgacgat cctgccgccc gagccgacgc
    21661 ccagaatgcc tggcaccaaa cgttgcacaa agccgggtac atcggcttgt cgtttcccgt
    21721 cgaatacggc ggccacggcc tgccgccgat atatgaagcg atcctcaacg atgaactcgg
    21781 gcgcgcaggt gccccgccaa tcgaaggggt cggccacctc agtaatgcgt tgcggctctt
    21841 cgggtccgat ctacagcgcg ccgaactact gcccggcctg atgtcaggcg cggtccggtg
    21901 gtgccagggg ttcagcgaac cggaagcggg ctcggacctg gctagcctga aaacgcgagc
    21961 cgacttgatc gacgtcgatg gtcgggacgt attccgcgtc aatggacgca agatctggac
    22021 cagcttcgcg gcagtcgctg actggtgttt cctgttgtgt cgcaccgagc cagacgctcg
    22081 gaagcacgaa ggaatctccg tgctgcttgt gccgatgtcg acgccaggta tcgacgttcg
    22141 accaatcgtc aacgccgcgc gcaaccgcga attcaccgag gtgaccttcg acgatgtcga
    22201 cgtacccgct gaaaacctgc tgggtgagcg aggccagggc tggtcgatcg ccacccagtt
    22261 gctcgcatac gaacgcgggc cgagcgatat caactggatc agccgactgt cgaggcaact
    22321 gcgaagcctc gagagagatg tacgactcgg ccggctcgaa aactctccga tgacccgggt
    22381 acgtctcgga caggcctatg ccgaactccg cgcattgcag atgaaagtgc agcgttcact
    22441 gagcgatcga cagcgcggcg cccttcccgg cccggaggga tcggtcgaca agctgctgat
    22501 ggcgcgcgcc gaccaggtcg tcggccacac catgatggac ctcaccgaca gcgagccgct
    22561 gctcacggag gggctggagt gggatatcta tgtctggtcc cgggccgctg ggatttacgg
    22621 cggcaccgcg cagattcagc gcaacatcgt tgctcagcga gtgctcgggc tgccgcgaac
    22681 cacatagttc agcccgcgcc accgctgccg gtcatctgtg catgagcctc ctcgagggct
    22741 gcaagtcgtc gcagcatgac ctcgtcggcg tcgccggcag ccagctttgc ctggatctcc
    22801 ttgatctcga tggcaaggcg ttcggcggct tccgcgcggg tcaacaagtc ctgatcggcc
    22861 cagtcatcgt gcggaatccg gtccttcggg tccataggcg actcctcgca ctcgaccgtc
    22921 agttgagatc aggctagctc cgacggtata gtcaactgat cagatagtgt ctaattgttc
    22981 catagactat cagccggaag gaccgggtac gagcgatgcc gctacaaccg tcgatgaagc
    23041 tgctctcggt cgatgaccac ctcatcgagc ccccgcacgt ttggtcggac cgacttccca
    23101 agaaatatct gcacgacggc ccgcgcatcg tcgagttccc gcgcgagggc aagcccccga
    23161 tgcaccagtg ggtctacgag ggccgcagct atccgaacat cggactcaac gccgtcgcag
    23221 gcaaatcccc cgaggagttc ggcgtcgacc ccgtccgcta cgacgacatg attcccgggt
    23281 gctacgaccc gaaagcccgt ctgatcgaca tggacatcga cggcgtccat gcgatgttgt
    23341 gctttccgtc attcccccgg ttctgcggca cggttttcct cgagggcacc gacaaggacc
    23401 tggcgctcct ctgcgtccag gcatggaacg acttctcgct cgacgaatgg tgcgccgccg
    23461 atccagcccg gttcatcccg atgatgatca ctccgttgtg ggatacgcag ttgatggtca
    23521 ccgagatcga acgagttgcc gcaaagggtt gtcgcgccgt gggtctgccc gacaacccca
    23581 tgaaccttgg tctgcccagc ttccacacgt cccattggga tccggtttgg tccgccctcg
    23641 aggaaaccaa catgacggcg gtcatgcact tcggttccgg cggcatgccg ccttccaccg
    23701 cagccgaggc gccgttcgcg gtgatggtca cgctgatggg cacgacttcg atggcggccg
    23761 ctgtcgagtt ggtgttctcg ccggtgttcc acaagcaccc gaacctcaag gtcgcgttct
    23821 ctgaaggcgg gatcggatgg atgccgtacc tcgtcgagcg cgcggactat gtgtggcgca
    23881 agcacaagta ctaccagaac atccatccga ccgtcgcgcc atcagagctg ttccgccgca
    23941 acataaccgg ctgcttcatc gaggacgaag tcggggttgc gatgcgccat cagattggca
    24001 tcgacaacat cacctgggag tgcgattacc cgcattccga ctcgttctgg ccgaagagtc
    24061 gcgcccgcgc cgaggagatg ctgtcgaaag tgcccgacga ggacgcccac aagatcgtgg
    24121 aactcaacac gcggcgttgg tacgcgtttc ccgaggaagg cttcaaagcg tcaaccgccg
    24181 atagcggatg gcggcccaat gacgggaatc caccggagta cgactatgac gccgtgatgt
    24241 ccgatcacgg tggggtcggg tacggagcct tcatccaaaa ccttgccgac cagatggcca
    24301 acaaggagaa ctgagcgcga ggctaactcg gcgtggcgat ttcagtcgct gtgtcacgac
    24361 cacgcaggac ttgttggccg acaacatgcc atcgccgcgc ctctgaatct gatgcgcgtt
    24421 gcaacgcagc ggcggaggcc actacgccac ccgactcctt cgcgacgtcg ctaagacgcg
    24481 ctgcctcatt gaccgggtcg ccgatcaccg tgtactcgta gcgactgtgg ccgccgacgt
    24541 tgcccgccgc tgccttgccc gcggacactc ctatgccgaa cttgatttcg gtgctagacg
    24601 ggttcaaccg ggaaccgagt tccctcgcgg cggccaacgc acggccggcg ttgtcgtctg
    24661 tcgatacggg agcgccgaag atagccagcg cggcatcacc ctggaacttg ttgatcagac
    24721 cgccgtgcct ctcaatcact tccacgacaa ccgcaaagaa gtcattgagg atcgagacaa
    24781 cgtcttcggg cgaaagcgcg gcggccagct gtgttgagcc gaccatatcg atgaacagca
    24841 ctgctatttc gcacttctcg ccacccatcg caatgtgagc gttctcctcg gcgagtcggg
    24901 cgacgtcacg gccaacatga cgcccgaata ggtcgcggag tcgctcgcgt tcacgtaagc
    24961 cgcccgccat gatgttgaat ccggcctgca atagcccgag ctccgatgca tcgaacacgg
    25021 gaacagatac gtcgtactcg ccgcgctcga cacggcgcat accgctcctg acctcgtcga
    25081 tgggatccgc tgtggtcgcc ccgattaaag ctgccacagc gagaccgcag gcaacggtaa
    25141 gcgctccaag cgccaatacc acccaagcca atcggtctcg gggatagtcg accgccagcg
    25201 cgctggcggc aaccagcatc agccccatca tcggaatgcc ggtacccacg acccacgcac
    25261 cgaccgcgcg tgcgcgaagg ccgggtccgc gcggcctcgt cggtgggtta gtggtcagca
    25321 attccgcgac agcctctcgc agggcgcgcg cacacaacca gtacgacaac ggtgcggtca
    25381 ccgctgcgcc gaggagtacg gagatgccca acgttgcggc taaccagggt gtgccgaggt
    25441 tgatcaccac caagagaagg gccgccgcgg tccacaccgt cgcgtggatc acggccatcc
    25501 gtaccggaac gacgaggagc gtgcggcgac cgtgcgcctc atcggcgtcc gcaggaaggg
    25561 tcaaccagaa ata
//
LOCUS       DASRJA010000376         4333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2137457,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000376 DASRJA010000000
VERSION     DASRJA010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137457"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VFC01_16685"
     CDS             <1..865
                     /locus_tag="VFC01_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN81304.1"
                     /translation="QEISQFFNTFQSASSALEKLAGVLAERPGIKDPPRPVLLDKVRG
                     DVAFDDVQFSYVQGRPVLPDLSFSVPAGQTVALVGTTGAGKTTIAKLIARFYDPTSGT
                     VTLDGIDLRDISQSELRRHVVMVTQENFMFDGTVADNIRFGRPEATDAEVVAAAAAVG
                     ADRFIDVLPDGYDTDVAKRGGRLSAGQRQLVAFARAFLADPAVLILDEATSSLDIPSE
                     RMVQRALETVLSDRTSLVIAHRLSTVQVADRVLVLDHGRIVEDGAPDDLIGRAGGRYA
                     ALHRAWVESLA"
     gene            complement(935..2311)
                     /locus_tag="VFC01_16690"
     CDS             complement(935..2311)
                     /locus_tag="VFC01_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HZN81305.1"
                     /translation="MGRTLEADYLVVGAGAMGLAFIDTLVSESNATVVVVDRNDRPGG
                     HWTTAYPFVRLHQPSAYYGVNSRNLGTDTIDQDGLNAGYYELASGAEVCAYFDAVMRQ
                     HLLPTGRVTYLPMCEYRGDGRIRKLGGDEIGVTARRVVTSHVEIVVPSMRSPSYAVDG
                     VDCVPPNDLPRIREARDRYVVVGAGKTAMDACTWLLRHGIPAQRLTWIKPRDSWILDR
                     AAVQPGSQFAKRVLRDFSSQLAAVHEAESLPDLFDRLETKGCLMRIDQSVEPTMYRCA
                     ILSQPELDALRGIDDVVRMGHIKSIEPGRITLEGGTRDIEDSALYIDCTADGFAHRDP
                     TPVFTDDHISLQAVRTCQPAFSAAVIGHVEATYPDDETRNEFCGPVPYPREPTDWLRM
                     MSAFNKNQLQWFSDPDMMAWVDASRLNVLHHVSAAVSDRAREKIISVLTSQLPAINDK
                     LESLLAQA"
     gene            complement(2312..3421)
                     /locus_tag="VFC01_16695"
     CDS             complement(2312..3421)
                     /locus_tag="VFC01_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HZN81306.1"
                     /translation="MADINPYRISVPDADLVDLKDRLARTRWPEAECVDDWSQGIPLS
                     YTRELADYWAGEYDWRAREAALNRFDHFTTEIDGLDIHFIHQRSSDDNAFPLLITHGW
                     PGSIVEFHKVIEPLTAAGFHVVCPSLPGYGFSGKPSQTGWGVERIATAWETLMGRLGY
                     ERYGAQGGDWGAAVTTQIGRNGGRCAAIHLNMPLGRPPGPLDNPTDEEKQALDRVGYY
                     RKWDNAYSKQQSTRPQTVGYGLVDSPVGQLAWIVEKFWSWMDCDGHPENVLTRDELLD
                     NVMLYWATASAASSARLYWESFRVFGGGEPDRVELPTGVASFPKEILRAPRSWCEAGY
                     NITHWTTMPRGGHFAAFEQPELFVDDVSAFFATVR"
     gene            complement(3427..4101)
                     /locus_tag="VFC01_16700"
     CDS             complement(3427..4101)
                     /locus_tag="VFC01_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81307.1"
                     /translation="MPQNPMTMGGTPSPRDHIAAVVAAHGALTNAIAEASRLMTANAR
                     GAYAAHLDDHRAELNVAVGELALWLDSFRGWAPVDVGEGIQPAATGPLPVDTSFEAEL
                     RTARETLKARRKDLLGQLAKARAALDRYGLPAEEITAYRRVIRVWAGEAIDVVAAVHR
                     LALADRYIRRLGRLAGDEALHRDGTELLRRWMHELEEVDREGELALAETCGYRELVER
                     YRAEAV"
     gene            complement(4157..>4333)
                     /locus_tag="VFC01_16705"
     CDS             complement(4157..>4333)
                     /locus_tag="VFC01_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN81308.1"
                     /translation="ELDVLAQVALGCTNDEAAKRLSLKPETVKSYLRNATAKLGAHTR
                     HEAVAKARRARLLP"
BASE COUNT          686 a         1473 c         1411 g          763 t
ORIGIN      
        1 gcaggagatc tcgcagttct tcaacacctt ccagtccgcg tcttcggcgt tggagaagct
       61 ggccggtgtg ttggccgaac ggccgggcat caaggacccg ccgcgccctg tcctgctcga
      121 caaggtgcgt ggcgacgtcg cgttcgacga cgtgcaattc tcctacgtgc agggccggcc
      181 cgtgttgccc gacctgagct tctcggttcc ggccggacag acggtcgcgc tggtcggcac
      241 taccggcgcg ggcaagacca cgatcgccaa gctgatcgcc cggttctacg acccgacgtc
      301 gggcacagtg acgctggatg gcattgacct gagggacatt tcgcagagcg agctgcgtcg
      361 ccacgtcgtg atggtcaccc aggagaactt catgttcgac gggactgtcg ccgacaacat
      421 ccggttcggc aggcccgagg ccaccgatgc ggaggtggtt gcggccgccg cggcggtcgg
      481 cgcggaccgg ttcatcgacg tgctgcccga cgggtacgac accgacgtcg ccaagcgcgg
      541 cggccggctc tccgcagggc agcggcagct cgtcgcgttc gcccgcgcgt tcctcgccga
      601 ccccgcggtg ctgatcctcg acgaggccac gtcgtccctc gacatcccca gcgagcggat
      661 ggtgcagcgg gcgctggaaa ccgtgctgtc cgaccgtact tcgctcgtca tcgcccaccg
      721 gctgtccacg gtgcaggtcg ccgaccgggt tttggtgctc gaccacggcc gcatcgtcga
      781 agacggcgcg cccgacgatc tcatcggccg tgccggcgga cggtatgccg cgctgcaccg
      841 cgcgtgggtg gagtcgctcg cctagtccgt gaatctgtgg ggagatcgcg gttctgcgcg
      901 tttttgcgat ctgcccgcag tctcggcgac gccgtcacgc ctgtgccagt agcgattcca
      961 gtttgtcgtt gatggcgggc agctgcgaag tgagcaccga gatgatcttt tcgcgcgccc
     1021 ggtcgctgac agccgcggac acgtggtgca acacattgag ccgcgaggcg tcgacccacg
     1081 ccatcatgtc tgggtccgaa aaccactgca gctggttctt gttgaacgcc gacatcattc
     1141 gcagccagtc cgtcggctca cgcggatagg gcaccggccc gcagaactca ttcctggtct
     1201 cgtcgtcggg atacgtcgcc tcgacgtggc cgatgacagc cgcgctgaac gccggttggc
     1261 aggtgcgcac ggcctgcagc gaaatgtggt catcggtgaa caccggggtc gggtctcgat
     1321 gcgcgaatcc gtctgccgtg cagtcgatgt agagcgcgga gtcttcgatg tcgcgggttc
     1381 cgccttcgag tgtgatgcga cccggctcga tcgactttat gtgtcccatc cgcaccacgt
     1441 cgtcgatgcc gcgcagcgcg tcgagttcgg gctgtgacag gatcgcgcag cggtacatcg
     1501 tcggttcgac ggactggtcg atccgcatca ggcagccttt ggtctccagc cggtcgaaca
     1561 gatccggcag cgactcggcc tcgtgcaccg cggccaactg gctggagaag tcacgcagca
     1621 ctcgcttggc aaactgcgaa ccgggctgaa ccgcggcgcg gtccaatatc caggagtcgc
     1681 ggggcttgat ccacgtcaac cgttgtgccg ggatgccatg tcgcagcagc caggtgcacg
     1741 cgtccatagc ggtcttgccc gcgcccacca ccacgtagcg gtccctggcc tcgcggatgc
     1801 gcggcagatc attgggtggc acgcagtcca cgccgtcgac cgcgtaggac ggcgagcgca
     1861 tcgacggcac cacgatctcc acgtggctcg tgacgacgcg gcgcgcggtc acgccgatct
     1921 cgtcgccgcc gagcttacgg atccggccgt cgccgcggta ttcgcacatc ggcaggtagg
     1981 tcacccggcc cgtcggcagc aggtgctgac gcatcaccgc gtcgaaatac gcgcacacct
     2041 cggcgccgct ggccagctcg tagtagccgg cgttcagccc gtcctggtcg atggtgtcgg
     2101 tgccgaggtt ccgcgagttg acgccgtagt acgccgacgg ttgatgcagc cgcacgaacg
     2161 gataggccgt cgtccagtgg cctcctggcc ggtcattgcg atccaccacg acgaccgtgg
     2221 cgttggattc gctgaccaga gtgtcgatga acgccagccc catcgcgccg gcgcccacca
     2281 ccaggtagtc cgcctccaga gtcctcccca ttcagcgcac ggtagcgaag aatgcactca
     2341 cgtcgtcgac gaacagctcg ggctgttcga atgccgcgaa gtgtccgccc cgcggcatcg
     2401 tcgtccaatg ggtgatgttg tagccggcct cgcaccagct gcgcggcgcg cgcaggatct
     2461 ccttggggaa cgacgcgacg ccggtgggca gctcgacgcg gtcaggttca cccccgccga
     2521 agacgcggaa gctctcccag tacagccgcg ccgacgacgc cgccgacgcg gtcgcccagt
     2581 acaacatcac gttgtcgagc agctcgtcac gagtcagcac gttctccgga tgaccgtcgc
     2641 agtccatcca cgaccagaat ttctcgacga tccacgccag ctgcccaacc ggtgagtcca
     2701 ccaatccgta gccgacggtc tgcgggcggg tcgactgctg tttggaatag gcattgtccc
     2761 atttgcggta gtagccaacg cggtccagcg cctgcttctc ctcgtccgtc gggttatcca
     2821 ggggtccggg cggcctgccc agcggcatgt tcaagtggat cgccgcgcag cgtccgccgt
     2881 tgcggccgat ttgcgtggtg acggcggcgc cccagtcccc accctgcgcg ccgtagcgct
     2941 cgtagccgag gcggcccatc agtgtctccc aggccgtcgc aatccgctcg acgccccaac
     3001 ccgtttgcga cggcttaccg gagaagccgt atccgggcag tgacgggcag accacgtgaa
     3061 agcctgcggc ggtcagcggt tcgatcacct tgtggaactc gacgatcgac ccgggccagc
     3121 cgtgcgtgat cagcagcgga aacgcgttgt cgtcggacga gcgctggtgg atgaaatgga
     3181 tgtcgaggcc gtcgatctcg gtggtgaagt ggtcgaaccg gttcagcgcg gcttcgcgtg
     3241 cgcgccagtc gtattcacct gcccagtagt cagccagctc gcgggtgtag ctcagcggga
     3301 tgccctggct ccagtcgtcg acgcactcgg cctccggcca tcgggtgcgg gccagcctgt
     3361 ccttcaggtc gacgagatcc gcgtcgggaa ccgaaatgcg atacgggttg atgtcggcca
     3421 tggcaatcac actgcctctg cccggtagcg ctcgacaagc tcccggtagc cgcacgtttc
     3481 ggccagcgcc aactcgcctt cgcggtcaac ctcttccagc tcgtgcatcc atcggcgcag
     3541 tagttctgtt ccgtcgcgat gcagcgcctc gtcgccggcg agccgtccca gccggcggat
     3601 gtagcgatcg gcaagcgcca accgatggac ggcggccacc acatcgatgg cttcgcctgc
     3661 ccacacccgg atcaccctgc gatatgcagt gatctcctca gccggcagcc cgtagcgatc
     3721 cagggccgcg cgagctttgg ccaactggcc gagcaagtct ttgcgccggg ctttgagggt
     3781 ttcacgcgcc gtccgcaact ccgcttcgaa tgacgtgtcg accggcagcg gacccgtcgc
     3841 agccggctgg atcccctcac cgacatcaac aggggcccag cccctgaaac tgtccagcca
     3901 caaagccaat tcgccgaccg ccacgttgag ctcggcgcga tgatcgtcga ggtgcgccgc
     3961 gtacgcgccg cgcgcgttgg ccgtcatcag tcgggacgct tcggctatcg cgttggtcag
     4021 ggcgccatgg gcagccacca ccgcggcgat gtgatcgcgg ggcgacgggg tgccacccat
     4081 agtcatcgga ttctggggca tggggactcg tacccatata agcatggttg cgagtgtggc
     4141 ggtcgagcag agcccgtcac ggcagcagcc gcgcccggcg cgcctttgcc acagcctcgt
     4201 gccgggtgtg cgcgccgagc ttggccgtcg cgttacgcag atagctcttc acggtctctg
     4261 gcttcagcga aagacgtttc gccgcttcgt cattcgtgca acccagcgcg acctgagcga
     4321 ggacgtcgag ctc
//
LOCUS       DASRJA010000377         7100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1478026,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000377 DASRJA010000000
VERSION     DASRJA010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478026"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..766
                     /locus_tag="VFC01_16710"
     CDS             281..766
                     /locus_tag="VFC01_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZN81309.1"
                     /translation="MEFDVVIEIPKGSRNKYEVDHDTGRVKLDRYLYTSMAYPTDYGF
                     FENTLGEDGDPLDALVLLPEPVFPGCIIEARVVAMFQMTDEAGGDDKVLCVPAGDPRW
                     DHIKDLGDVPAFELEAIKHFFVHYKDLEPGKYVKAADWVGRDKAEAELQASIERYKAT
                     H"
     gene            855..1376
                     /locus_tag="VFC01_16715"
     CDS             855..1376
                     /locus_tag="VFC01_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HZN81310.1"
                     /translation="MLLHQGIGLDAFNAMPMRRAVHAIYECCYSVPLAADLARGRSYD
                     SHDQLFRQADALLFGLGEDSIDSILQAYPDIGKRPGSEKSQAEQCAIWCDQPEVMDQL
                     TVASKRYLEHFGFGFMMFINGYTAVDVLATMTDRLLNDLETERKVVRNELSRINRTRL
                     ERMLGPEGGYDNW"
     gene            complement(1381..1914)
                     /locus_tag="VFC01_16720"
     CDS             complement(1381..1914)
                     /locus_tag="VFC01_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81311.1"
                     /translation="MKIRTMAGGFATAAVLTMGGFGYAAAQPGDFSTLPVDPNLITDS
                     LAYSAAPPVFNPNGQPGVQAVYTHRDGSRQITSTVLVLPDAQAASAALDGARAELAGK
                     VANGKTQAAAVGSGGTMLTGTSPDGSKSVTVLSFAEGNTATTLEFDGASTDPAPSDLV
                     LELGRKQDTAIRDWQAA"
     gene            complement(1933..2172)
                     /locus_tag="VFC01_16725"
     CDS             complement(1933..2172)
                     /locus_tag="VFC01_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81312.1"
                     /translation="MRKIGMALAIAAVLSGSVATAQTAYAAQSSHDTNVSIQPVLPAG
                     PKLQLLDCYGTTGGMGCGPGWVWRDGWRGMGCYPC"
     gene            2311..3462
                     /locus_tag="VFC01_16730"
     CDS             2311..3462
                     /locus_tag="VFC01_16730"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN81313.1"
                     /translation="MDNVVHGHCDNRFGNVADALADEIAKGEELGASIALDIDGELVV
                     DIWGGHADRAKTVPWGHDTIANFFSCTKTLTALAALIAIDRGLVDAFAPVAKYWPEFA
                     DNGKQDIEVRHLLSHTSGVSGWEPPFGIEDIYDWEKATGHLARQAPWWPPGTASGYHA
                     VNYGHLIGEVIRRATGKTLKAFVRDEIAQPLGADVQIGARDDDYHRIAELIPPPPLDL
                     PLDAMPADHPMIKTFAAFPPDSNGAAIAETTEWRRADIGAANGHGNARALARALSPIS
                     LGGKANGVQLLSQDTVDLIFEEQSNGLDLVLMIPLRFGVGFGLPCPESVPAIPDGKIC
                     FWGGWGGSMAVMDLDRRATITYVMNKMGPGTTGTERTNRYARLIYEALG"
     gene            complement(3444..3842)
                     /locus_tag="VFC01_16735"
     CDS             complement(3444..3842)
                     /locus_tag="VFC01_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81314.1"
                     /translation="MRLFVADAPGGDWTELTDGDQRTVRVAAADLQQARRARSRIRAG
                     GDDLAVILDITVAVADDFRSARSVLGPAEHTVHYAGTVDGLAGLISDIAGADVADGVT
                     LISASPQQDLRDLARDVLHRLASRDQPRAS"
     gene            complement(3887..4888)
                     /locus_tag="VFC01_16740"
     CDS             complement(3887..4888)
                     /locus_tag="VFC01_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HZN81315.1"
                     /translation="MHVTQLEWIITLGVTIAVLLFDVIVIGRRPHEPSKRETGTALAI
                     YIGLAVAFGLWTWFYHGSQFGLEFFAGWLTEYSLSVDNLFIFLIIMASFKVPKKYQQQ
                     ALLVGIILALVFRGIFIALGAVAINQFSWVFYLFGAFLVYTAINLARDTEHDDDAENV
                     VVRFARRHLKLTDRWEGLRLWVKENGKRLMTPMFLVIVALGTTDLIFALDSIPAIYGL
                     TQQPYLVFTANVFALMGLRQLYFLLGDLLKRLVYLSQGLAFILAFIGVKLLLHALHEN
                     ELPFINGGEHVPVPEIPTLLSLGVIIVTLIITTVASLYKTRVHDVKKEAEKESLDSA"
     gene            5081..5722
                     /locus_tag="VFC01_16745"
     CDS             5081..5722
                     /locus_tag="VFC01_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZN81316.1"
                     /translation="MADTQLPGVFGDVQPMLGQYGYLGVGGMLFLEDFGVPVPGEIML
                     IAAAVFAGAGQMNIAVVFVVAVLAAVIGDNIGFVVGHFGGRPLAERFGRYVFLTPQRL
                     DRAEAFFNQHGGKVVTVARFVDGLRQINGLLAGIAGMHWLKFLGYNALGAALWVGTWC
                     TAGYLAGEHIVEIYDTFERYKWYVIAAVVVVVAIVITHRVRHAQSRGDAAPTA"
     gene            5735..>7100
                     /locus_tag="VFC01_16750"
     CDS             5735..>7100
                     /locus_tag="VFC01_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HZN81317.1"
                     /translation="MTAPRPEIPAQYLLSSFAPEPRTLIDILYATATRYPDAPALDDG
                     AVQLTYAELISDIEESVEWLAARGIGRGDRIGIRMPSGSYALYVAILSTLATGAAYVP
                     VDADDPDERAELVFGEADVVAVIGEQGLVRGPGSSRGWRAGAPLGRDDAWIIFTSGST
                     GTPKGVAVTHRNAAAFVDAEAQMFLQDNPIGPGDRVLAGLSVAFDASCEEMWLAWRHG
                     ACLVPAPRSLVRSGMDLGPWLVSRDITVVSTVPTLAALWPAEALEAVRLLIFGGEACP
                     PELAERLAVESREVWNTYGPTEATVVACAARLDGAGPVSIGLPLQGWDLAVVDKHGVP
                     VSAGDVGELVIGGVGLARYLDPAKDAEKYAEMPTLGWSRAYRSGDLVRLESDGLYFVG
                     RADDQVKVGGRRIELGEVDAALVNLPGVSGGAAAVRKTASGTPLLVGYVASADPSFDI
                     GKARA"
BASE COUNT         1230 a         2296 c         2337 g         1237 t
ORIGIN      
        1 aggcacgatg ccgggcttgg cggtcgccgc cgcaggcagt ggtttggcgg cttgcgcgtc
       61 gctggtgggg ttccccgtgg tgagcaccgc ggccacggcg acgacggcgg ccactagcag
      121 cagcaccgcc acgccgatca ccacatgcgt ggatcgccgc cactgcgtcg gccgcattaa
      181 ctctcctgct ctgttgaccg cccccttaca gcagcgtatc gctccgttag ggtgagcccc
      241 gtccctctag accggagcac atgggtaagg agccgcggcg gtggagttcg acgtcgttat
      301 cgagatccct aagggttcgc gcaacaaata cgaggtggac cacgacaccg gccgcgtgaa
      361 gctggaccgc tacctctaca cgtcaatggc gtatccgacg gattacggct tcttcgagaa
      421 cactcttggc gaggatggcg acccgctcga cgcgctcgtt ttgctgcccg agccggtgtt
      481 tccgggctgc atcatcgagg cgcgggtggt cgcgatgttc cagatgaccg acgaagcggg
      541 cggcgacgac aaggtgctgt gcgtgccggc aggcgacccg cgatgggacc acatcaagga
      601 cctcggcgac gtgcctgcct tcgagctgga ggcgatcaag cacttcttcg tgcactacaa
      661 ggacctcgaa ccgggcaagt acgtcaaggc cgctgactgg gtgggccggg acaaggccga
      721 ggcggaattg caggcgtcca tcgagcggta caaagcaacg cactaattga tgctcctttc
      781 gtccgcgcac atgatttttc attgccgtaa cacgcggtaa cgcgcgcgtg ccatgcgttt
      841 ccgatgatgg cggtgtgttg ctgcatcagg ggatcggcct ggacgcgttc aacgcgatgc
      901 cgatgcgacg ggccgtgcac gcgatttacg aatgttgcta cagcgtgccc ctggccgccg
      961 acctggcccg tggccggtcg tatgacagcc acgatcagct tttccggcag gccgacgccc
     1021 tgttgttcgg actcggcgaa gactcgatcg actccatcct gcaggcctat cccgacatcg
     1081 gaaagcggcc gggcagcgag aagtcacagg ccgagcagtg cgcgatctgg tgcgaccagc
     1141 ccgaggtgat ggaccagctg acggtggcgt ccaagcgcta cctagagcat ttcggcttcg
     1201 gcttcatgat gttcatcaac ggctacaccg cggtggatgt ccttgcgacc atgaccgacc
     1261 gtctgctcaa cgacctcgaa accgagcgca aggtggtgcg caacgagctg tcgcgaatca
     1321 accgcacgcg gctcgagcgc atgctcggtc ccgaaggcgg ctacgacaac tggtgacgct
     1381 ctaggcggcc tgccagtccc ggatcgcggt gtcctgcttc cgcccgagtt cgagcacgag
     1441 atctgatggc gcgggatctg ttgacgcacc atcgaattcg agcgtcgtgg ccgtgttgcc
     1501 ctcggcgaac gacagcactg tcacggactt cgatccgtcc ggtgacgtcc cggtcagcat
     1561 cgtcccgccg gacccgaccg ccgccgcctg cgtcttgccg ttggcgacct tgcctgccag
     1621 ttctgctcgt gcgccatcca gcgcggcgct tgccgcctgg gcatcgggca acacgaggac
     1681 ggtgctggtg atctgccgac taccgtcgcg atgggtgtag acggcctgca cgcccggctg
     1741 cccattcggg ttgaagacgg gtggcgccgc gctgtaggcg agcgaatcgg tgatcagatt
     1801 aggatcgacc ggcagcgtgc tgaagtcacc gggttgtgct gcggcataac cgaaaccgcc
     1861 catcgtcagt accgcagcgg tcgcgaaccc accagccatt gtgcgaattt tcatcacggc
     1921 atgctccaat cgtcagcagg ggtagcagcc catgccgcgc cagccatcac gccagaccca
     1981 gccgggccca cagcccatgc cgccggtggt tccgtagcaa tcgaggagct gtagcttcgg
     2041 cccggcgggc aacaccggct ggatggacac attcgtgtcg tgtgagctct gtgccgcgta
     2101 ggccgtttgc gcggtggcca ccgaacctga caacacggca gcgattgcaa gggccatgcc
     2161 gatcttgcgc atccgattcc tccctgtgtg tctgggaatt tgcagtgcaa acggtacgcc
     2221 gcagtcactc ggtacttgat gcattcgaaa aatactgacg ggcgactgga agccacccga
     2281 aaaagattgg ccccggcgca tgatgtcggg atggacaacg tcgtgcacgg ccactgcgat
     2341 aaccgattcg gcaacgtcgc ggacgcgctt gccgatgaga tcgccaaagg cgaggaactg
     2401 ggcgcctcga tcgcccttga catcgacggc gagcttgtcg tcgacatctg gggcgggcac
     2461 gccgaccgcg ccaagactgt gccgtgggga catgacacca tcgccaactt cttctcctgt
     2521 acgaagacac tgaccgctct cgccgccctg atcgcgattg atcgcgggct ggtggatgct
     2581 tttgcgccgg tggcgaagta ctggcccgaa ttcgccgaca acggcaaaca ggacatcgag
     2641 gtgcgccacc tcctgtcgca cacatcgggc gtttccggat gggagccgcc attcggtatc
     2701 gaggacatct acgactggga gaaggcaaca ggccatctgg cgcgacaggc accgtggtgg
     2761 ccacctggaa ccgcatccgg ctatcacgcg gtcaactatg gccacctgat cggcgaggtg
     2821 atccgccgcg ccaccggcaa gacgctcaag gccttcgtcc gtgacgaaat cgcccagccg
     2881 ctcggtgccg atgtccagat cggggcacgg gacgacgatt atcaccggat cgcggagttg
     2941 attcctccac cgccactgga tcttccgttg gacgccatgc cagcggacca tccgatgatc
     3001 aagacgttcg ccgcgttccc gcctgattcg aatggcgcag caatcgcgga gacaaccgaa
     3061 tggcgtcgag cagacatcgg cgccgccaac ggccatggaa acgcccgtgc attggctcgc
     3121 gcgctctcac cgatttcgtt gggcggcaag gcgaacggtg tccagttgct gagccaggac
     3181 accgtcgacc tgatcttcga agaacaatcc aacggcctcg acctcgtact catgatcccg
     3241 ttgcgcttcg gtgtcggctt cggattacct tgtccggaaa gcgttcccgc gattcccgac
     3301 ggcaagatct gcttctgggg cggctggggt ggctcgatgg cggtgatgga cctggatcgg
     3361 cgcgcgacga tcacctatgt gatgaacaag atgggcccgg gtaccaccgg tacggagcgc
     3421 accaaccgat acgcgcgact tatctacgag gccctgggct gatcgcgcga tgcgagccgg
     3481 tgcaacacgt cgcgcgcgag gtcacgcaga tcctgttgtg gtgacgccga aatcagcgtg
     3541 acgccgtcgg cgacgtcggc gccggcgata tcggagatca agcccgccag cccgtcgact
     3601 gtgcccgcgt agtgcaccgt atgctcagca ggcccgagaa cgcttcgtgc cgaacggaag
     3661 tcgtcggcca ccgccacggt gatgtcgagg atcacggcca agtcgtcacc accggcccgg
     3721 attcgcgagc gtgcccgtcg ggcctgctgc aaatcggctg cggcgacccg caccgtgcgc
     3781 tggtccccgt ccgtcagttc ggtccagtca ccgcccggcg cgtccgccac aaatagccgc
     3841 acggcgccaa gctagagccg cggtccatcg gtgtcgatgg ttgtgctcag gcagaatcca
     3901 acgattcttt ttcggcttcc ttcttcacgt cgtggacccg ggtcttgtac aggctggcga
     3961 cggtggtgat gatcagcgtg acgatgatca cgccaaggct cagcagcgtc gggatctccg
     4021 gtaccggcac gtgctcaccg ccgttgatga acggcagctc gttctcgtgc agcgcgtgca
     4081 gcaggagctt cacgccgatg aaggcgagga tgaacgccag cccctgcgac aggtacacca
     4141 gccgcttgag caggtcaccg agcaggaagt agagctggcg caggcccatc agcgcgaaca
     4201 cgttcgcggt gaacaccagg tacggctgtt gggtcaggcc gtagatcgcg gggatggagt
     4261 ccaacgcgaa aatcaggtcc gtcgtaccga gggcgacgat gacgaggaac atcggcgtca
     4321 tcaggcgctt gccgttctcc ttgacccaca gccgcaggcc ctcccacctg tcggtgagct
     4381 tcagatgcct gcgcgcgaac cggacgacga cgttctccgc gtcgtcgtcg tgttcggtgt
     4441 cccgggccag gttgatcgcg gtgtagacca ggaacgcccc gaagaggtag aacacccacg
     4501 agaactggtt gatcgccacc gcgcccagcg cgatgaagat gccgcggaac accagcgcca
     4561 ggatgatgcc gaccagcagt gcctgctgct ggtacttctt cggcaccttg aagctggcca
     4621 tgatgatcaa gaagatgaac aggttgtcga cggacaagct gtactcggtg agccagccgg
     4681 cgaagaactc gaggccgaat tggctgccgt ggtagaacca cgtccacaac ccgaatgcga
     4741 cggcgagtcc gatgtagatc gctagcgccg tgccggtctc gcgcttggac ggttcgtgcg
     4801 gccgacgccc gatcacgatc acgtcgaaca gcagcacggc gatcgtcacg cccagcgtga
     4861 tgatccactc gagttgggtt acgtgcataa acaagcctcc ggtcgttgat caacgccgga
     4921 ggtctcttcc gccgaccgag tcggcccgca gtgccggtcg tccgatgacg gccgacgtgg
     4981 tgacgacacc gcggcgaagg aatactcccc tccaccttct attctgccag gcccgtggcg
     5041 tcgaccgaaa tcggggaccg atcaacttac ggttgcacgc atggccgaca cccaacttcc
     5101 tggtgttttc ggagacgtgc agccgatgct cgggcagtac gggtacctgg gcgtcggcgg
     5161 gatgctgttc ctggaggact tcggggttcc ggtgcccggc gaaatcatgt tgatcgccgc
     5221 cgcggtcttc gccggtgccg gccagatgaa catcgcggtg gtgttcgtgg tcgcggtgct
     5281 cgccgccgtc atcggcgaca acatcggctt cgtcgtcggc cacttcggtg gcaggcccct
     5341 cgctgagcgg ttcggccgat acgtcttttt gaccccgcag cggctggacc gcgccgaggc
     5401 gttcttcaac cagcacggtg gcaaggtcgt caccgtcgcc cggttcgtcg acggactgcg
     5461 acagatcaac ggcctgctcg ccggcatcgc cggcatgcac tggctgaaat tcctcggcta
     5521 caacgcgctc ggcgcggcgc tgtgggtggg cacctggtgc acggccgggt acctcgcagg
     5581 cgagcacatc gtcgagatct acgacacgtt cgagcggtac aagtggtacg tgatcgctgc
     5641 ggtcgtcgtc gtcgtcgcaa tcgtgatcac gcatcgcgtg cgacatgccc aatcgcgcgg
     5701 cgatgccgcg ccaacggctt agtagtttgt attcgtgaca gcgcctcggc cggaaatccc
     5761 cgcgcagtat ctgctgtctt cgttcgctcc cgaacctcgc acgctgatcg acatccttta
     5821 cgccacagcc acccgatatc ccgatgcgcc ggcgctcgac gacggcgcgg ttcagctcac
     5881 ctacgccgag ctgatctcag acatcgagga aagcgtcgag tggctcgccg cgcgcggcat
     5941 cggccgcggc gaccgcatcg gcatccggat gccgtccggt agttacgcgc tgtatgtggc
     6001 gatcctctcg acgctggcca ccggggcggc gtacgtgccg gtcgacgccg acgatccaga
     6061 cgagcgtgcc gagctggtat tcggcgaggc cgatgtggtc gcagtcatcg gcgaacaagg
     6121 actagtccgc ggacccggtt cgtcgcgcgg ctggcgcgca ggagccccgc tcggtcgcga
     6181 cgacgcctgg atcatcttca cgtccgggtc caccggcacc ccgaagggtg tcgcggtcac
     6241 gcatcgcaac gccgccgcgt tcgtcgacgc ggaagcacaa atgttcctgc aggacaaccc
     6301 cattgggccg ggcgaccggg tgctggccgg tctgtcggtc gcgttcgacg cctcatgcga
     6361 ggagatgtgg ctggcctggc ggcacggggc gtgcctggtg ccggcgccgc gctcgctggt
     6421 ccgcagcggc atggacctgg ggccctggct ggtgtcccgc gacatcaccg tggtgtcgac
     6481 ggtgccgacg ctggctgcgc tgtggcccgc cgaggcactc gaggccgtgc ggctgctgat
     6541 cttcggcggc gaagcatgcc cgccggagct ggccgagcgg ctggccgtcg agagccgcga
     6601 ggtgtggaac acctatggcc ccaccgaggc gaccgtggtg gcatgcgcgg cccggctgga
     6661 cggcgcgggg ccggtgagca tcggactgcc gcttcagggc tgggatctgg ccgtggtcga
     6721 caagcatggc gtcccggtca gcgccggcga cgtgggcgaa ctcgtcatcg gaggggtggg
     6781 gctggcccgc tacctggatc cggccaagga cgccgagaag tacgccgaga tgccgacgct
     6841 cggatggtcg cgcgcctacc gcagcggcga cttggtgcgc ctggagtccg acggcctgta
     6901 cttcgtcggc cgcgccgacg accaggtcaa ggtcggcggc cgccgcatcg agctcggtga
     6961 ggtcgacgcc gccctggtga acctgcccgg ggtcagcggc ggcgccgcgg cagtgcgcaa
     7021 gaccgccagc ggcacaccgc tgttggtggg atacgtcgcg agtgcggacc cgtcgttcga
     7081 catcggcaag gcccgcgccg
//
LOCUS       DASRJA010000378         4426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1725908,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000378 DASRJA010000000
VERSION     DASRJA010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725908"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFC01_16755"
     CDS             <1..251
                     /locus_tag="VFC01_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81318.1"
                     /translation="PTSPPLLPPPPQVITVPLKPPTVRPVRIQPGVVEQLVVAPTSGV
                     GEPLWGVAGLLLIPAAGAILGYRQARGAHAAAELGRHP"
     gene            complement(234..1844)
                     /locus_tag="VFC01_16760"
     CDS             complement(234..1844)
                     /locus_tag="VFC01_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-coumarate--CoA ligase family protein"
                     /protein_id="HZN81319.1"
                     /translation="MSFASPFPEVDIPSTNVYDFLFADIDDAHLDRVALVDAKSGRET
                     SYREMVGRIDAFAGALADRGIGVGDVIGLLSPNSSGFAVAFHGILRSGATATTVNALF
                     TAKDIAKQLTDSKAKMLVTVTPLLASAKEAAAAVGMADADLVVLDGEGLEATGHPNGA
                     DLMAKGLPAPQVSFAPSSHLAALPYSSGTTGNPKGVMLTHRNLVANVAQIRPLHGMVA
                     EDAVLAVLPFFHIYGMTVLLNAALHARARLVIMPSFDLGEFLGNIANHKCTIAFIAPP
                     VAVALAKHPLIDEYDLSSLNTVMSGAAPLDADLGHAVAKRLGCRVVQGYGMSELSPVS
                     HITPFDGGVRNMGMVAPLSSVGWTVSNAASKLIHPDTGDEIDLPAEGLSETGELWFKG
                     PNVMTGYLGNDQATRETIDDEGWLHTGDLAQVDACGCVYIVDRLKELIKYKGYQVPPA
                     ELEAVLLSHPSIADAAVVGVNDADGEEVPKAFVVKQSGAELTADEVIEFVAGQVAPYK
                     KVRQVEFIDAIPKSASGKILRKDLRVTT"
     gene            complement(1883..2287)
                     /locus_tag="VFC01_16765"
     CDS             complement(1883..2287)
                     /locus_tag="VFC01_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HZN81320.1"
                     /translation="MPHPIMFRDDDLGLAEVRKIALGFPEAFEKISWGRPVFCAPKIF
                     VMYGGSAKTDIKGEYDQFPYSILVKVDDSDRKALEQDTRFYFPAYMGPSGWLGLDFTA
                     RKKIDWDEVRELVDASFRLIAPKKLIKQLDEV"
     gene            2360..3793
                     /locus_tag="VFC01_16770"
     CDS             2360..3793
                     /locus_tag="VFC01_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HZN81321.1"
                     /translation="MIDRRGFLVLSALGTAAGVAGACATKSPAPTAEEASSKPVEVAL
                     NAAESEVDLGGVTVKTWTYTGTVPAKEIRLHKGQTLRAPVTNHLPQETSVHWHGLAIP
                     NAMDGVPVLTQPAIASGGNFTYEFVVPDAGTYYLHSHVGTQLDRGMYGPLIIEDPDEG
                     ADYDDELVVVLDDWIDGTGTTPDQVNENLKKTGMKPMAGQPPPLSPTLPLGEDGGDVT
                     YPYFLINGRTTKDPQVKDYRAGQRLRLRIINAGADTAFRVAVPTAELSVTHTDGYPVV
                     ARRTNAIILGMGERADAIVTVNSSVPVIAAAEGKDGYAQLNLRVGGAQPAVDVAEFVK
                     TLRTSEVLNTAVLTPAPGVVLPQRHPDQTVDLRLAGPVNGYTWPINGKLYDPPKNGIA
                     VAPGKRVRIRFISESMMFHPMHLHGHTFQVRQPTGPGARKDTVLVPPKQTVEVEFDTD
                     NPGRWIVHCHNDYHLDNGMATFLEYTG"
     gene            3862..>4426
                     /locus_tag="VFC01_16775"
     CDS             3862..>4426
                     /locus_tag="VFC01_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZN81322.1"
                     /translation="MLAEQADEAPDADLRRRWQELADEVREHQFRYYVRDAPIISDAE
                     FDKLLRELQGLEDEHPELRTPDSPTQLVGGAGFATDFAQADHLERMLSLDNVFAPEEL
                     TAWAARIKGEVGDDAHYLCELKIDGVALALVYRDGRLERAATRGDGRTGEDVTLNART
                     IDDVPEKLTGTKEFPVPRVLEVRGEVFF"
BASE COUNT          813 a         1430 c         1444 g          739 t
ORIGIN      
        1 aaccgacctc gccaccactg ctgccgccgc cgccacaggt gatcaccgtt ccgctgaagc
       61 cgccgaccgt caggcccgtc cgcatacagc ccggggtcgt cgagcaactg gtcgtcgcgc
      121 cgacgtccgg ggtgggagaa ccactgtggg gcgtcgccgg gctgctgttg atccccgcgg
      181 ccggtgccat cctcgggtac cggcaggcgc ggggcgcgca tgcggccgcg gaactaggtc
      241 gtcacccgta ggtccttgcg caggatcttg cccgacgcgg acttcgggat cgcgtcgatg
      301 aactcgacct gtcgcacctt cttgtagggg gcaacctggc ccgcgacgaa ttcgatgacc
      361 tcgtcggccg tcaactcggc accagattgc ttgaccacga acgcctttgg cacctcttcg
      421 ccgtcggcat cgttgacacc gacaacggcc gcatcggcga ttgacgggtg actcagcagc
      481 acggcttcga gttcggcagg cggcacctgg tagcccttgt acttgatcag ctctttgagc
      541 cggtcgacga tgtacacgca gccgcaggcg tcaacctggg ccaaatcccc tgtgtggagc
      601 caaccttcgt cgtcgatggt ttcccgggtg gcctggtcgt tgccgaggta gccggtcatc
      661 acgttgggcc ccttgaacca gagttcaccg gtctcgctga gaccctctgc cggaaggtcg
      721 atttcgtcgc cggtgtcggg gtgaatgagt ttggaggccg cgttggacac cgtccacccg
      781 accgagctca acggtgcgac catccccatg ttgcggacac cgccgtcgaa cggggtgatg
      841 tggctgaccg gactcagctc gctcatcccg tagccctgca ccacccggca acccaaccgc
      901 ttggcgacgg cgtggccgag gtcggcatcc aacggcgcgg cgcccgacat cacggtgttc
      961 aacgacgaca ggtcgtattc atcgatcagc ggatgcttgg ccagcgcgac ggcgaccgga
     1021 ggcgcgatga acgcgatcgt gcacttgtgg ttcgcgatgt tgccgaggaa ttcaccgagg
     1081 tcgaagctcg gcatgatcac caggcgtgcg cgtgcgtgca gggcggcgtt gagcagcacc
     1141 gtcatcccgt agatgtgaaa gaacggtaga acggccaaga ccgcgtcctc ggcgaccatc
     1201 ccgtgcagcg gccggatctg tgcgacgttg gcgaccaagt tgcggtgcgt cagcatcacg
     1261 cccttggggt tgccggtcgt tcccgagctg tagggcagcg ccgccagatg tgacgacgga
     1321 gcgaagctca cctgcggtgc gggcagtccc ttggccatca ggtcggcgcc gttcggatgt
     1381 cccgtcgcct ccagcccctc gccgtcgagc accacgagat cggcgtcggc cataccgacg
     1441 gcggcggcgg cctctttggc cgaggcaagc aacggtgtga cggtcaccag catcttggcc
     1501 ttggagtcgg tcagctgctt ggcaatatcc ttggcggtga acagcgcgtt gacggtcgtg
     1561 gccgtcgcac ccgatcgcag gatgccgtgg aaggcgacgg cgaagcccga gctgttgggg
     1621 gacagcagcc cgatgacgtc gccgacaccg atgccgcggt cggccagcgc gccggcgaaa
     1681 gcgtcgatgc gcccgaccat ctcgcggtag ctggtctctc ggccggattt ggcgtcgacc
     1741 agggctactc ggtcgaggtg ggcgtcgtcg atgtcggcga agaggaagtc gtagacgttc
     1801 gtcgacggga tgtcgacttc gggaaaagga ctcgcgaaac tcatagcgtc tccgatcgaa
     1861 tcatggccta ccgcttaccg gatcaaactt cgtcgagctg cttgatgagt ttctttggcg
     1921 caatcaaccg aaacgacgcg tcgacgagct cgcgcacctc gtcccagtcg atcttcttgc
     1981 gcgcggtgaa gtccaggccg agccagccgg acggacccat ataggcgggg aagtagaagc
     2041 gggtgtcctg ctcgagagct ttgcggtcag aatcgtcaac cttgaccagg atcgagtagg
     2101 ggaattggtc gtactcgccc ttgatgtcgg tcttggcgct accgccgtac atgacaaaaa
     2161 tctttggcgc gcagaacacc ggcctgcccc aggagatctt ctcgaacgcc tccgggaaac
     2221 ccaacgcgat cttgcgcact tccgccagcc cgaggtcgtc gtcgcggaac atgatcgggt
     2281 gcggcatggg tgtcagcgta gcgggtgcag actgtgcggt ttcgcgcctg agcagccgcg
     2341 acagtgacac gctgacgtca tgatcgatcg gcgcggtttc ctggtgttat cggcactcgg
     2401 gacggccgcc ggtgtggcag gcgcgtgcgc gacgaagtcc cccgcaccaa cggccgagga
     2461 ggcgagcagc aagccagtcg aagtcgccct caatgcagcg gagagcgagg tcgatctcgg
     2521 cggcgtcacc gtgaaaacgt ggacatacac cggcaccgta ccggcgaaag agatccggct
     2581 gcataaggga caaacgctgc gcgccccggt gaccaatcac ctgccgcagg aaacctcggt
     2641 gcattggcac gggctggcga tacccaatgc aatggatggg gtgccggtgc tgacccagcc
     2701 cgcgatcgcg tccggaggaa acttcaccta cgagttcgtg gtgcccgacg caggcactta
     2761 ctacctgcac tcgcatgtcg gaacccagct ggaccgcggc atgtatggac cgttgatcat
     2821 cgaagatccc gacgagggcg ccgactatga cgacgaactc gtcgtcgtgc tcgacgactg
     2881 gatcgacggc accggcacca ccccggacca ggtgaacgaa aacctgaaga agaccggcat
     2941 gaagccgatg gccggccagc cgccaccgct gagcccgacc cttcccctcg gggaggacgg
     3001 tggcgacgtc acctatccgt acttcctgat caacggcaga acaaccaagg atccgcaggt
     3061 gaaggattac cgtgccggac aacggcttcg gctgcgcatc atcaacgccg gcgccgacac
     3121 cgcattccgc gtggccgtcc cgaccgccga gctgagcgtc acgcacaccg acggctaccc
     3181 ggtcgtcgca cgtcgcacca acgcgatcat cttggggatg ggtgagcgcg ccgacgcgat
     3241 cgtcacggtg aattcgtctg tgccggtgat cgccgccgca gagggcaaag acggttacgc
     3301 gcagttgaac ctgcgtgtcg gcggggccca gcccgccgtc gacgtcgctg aattcgtcaa
     3361 gacattgcgg acgtcagagg tgctgaacac ggccgtgctc actccagctc ccggcgttgt
     3421 tctcccacaa cgccacccgg accaaacagt cgacttgaga ctcgcggggc cggtgaacgg
     3481 ctacacctgg ccgatcaacg gaaagctcta cgatccgccg aagaatggca tcgcggtggc
     3541 accaggcaag cgtgttcgca ttcgattcat cagcgagtcg atgatgttcc acccgatgca
     3601 cctacacggc cacaccttcc aggtgcggca gccgaccggt ccgggcgcgc gcaaagatac
     3661 ggtgctggtg ccgccgaagc agacggtgga agtcgagttc gatacggaca atccgggccg
     3721 gtggatcgtg cattgtcaca atgactatca cctcgacaac ggcatggcga cattcctcga
     3781 gtacaccggc tgacctggcg agcgtcgctg tcggcctgct cggctagcct caccagagtg
     3841 agcccgaaaa agccggaaac tgtgctggcc gagcaggccg acgaagctcc ggatgccgat
     3901 ctgcgccgcc gctggcaaga actcgccgac gaggtgcgtg agcaccagtt ccgctactac
     3961 gtgcgcgacg caccgatcat ctccgacgcg gagttcgaca aactgctgcg tgagttgcag
     4021 gggctcgagg acgagcatcc ggagctgcgc accccagact cgccgacgca actggtcggt
     4081 ggcgccggct tcgccaccga cttcgcccag gccgaccatc tcgagcggat gctcagcctg
     4141 gacaacgtgt ttgcgccgga ggaactgacc gcttgggccg cccgcatcaa gggcgaggtc
     4201 ggtgacgacg cgcattacct gtgcgagctg aagatcgacg gggtcgcgct ggcactggtg
     4261 taccgcgacg ggcgccttga gcgcgcggcc acccgcggcg acggccgcac gggcgaggac
     4321 gtcacgctaa acgcgcgcac cattgacgac gtgccggaga agctcacggg taccaaggag
     4381 ttcccggtgc cccgggtcct cgaggtgcgc ggcgaggtgt tcttcc
//
LOCUS       DASRJA010000379        15322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1515053,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000379 DASRJA010000000
VERSION     DASRJA010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15322
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515053"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VFC01_16780"
     CDS             <1..963
                     /locus_tag="VFC01_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN81323.1"
                     /translation="TLIHMDDPQHRVVRAIGADWFRPKAMRALKVRVDELAKRFVDQM
                     LAAGPECDFVQQVAVNYPLYVIMSMLGLPEEDFPRMLKYTQEMFGNDDPELQRGTTKE
                     EQMASLLDMFAYFTAVTASRRENPTEDLASAIANATIDDEPLSDIETVSYYSIVAAAG
                     HDTTSAVISGGLHALLEHPDQLARLQDNMDLMPLATEEMIRWVTPVKEFMRTAQADTT
                     VRGVPIAAGESVLLSYASANRDEDVFDDPFRFDVGRDPNKHNAFGYGVHFCLGAALAR
                     MEVNSFFSELVPLLESIELAGDPAHIATIFVGGLKHLPIRYSLK"
     gene            complement(1013..1609)
                     /locus_tag="VFC01_16785"
     CDS             complement(1013..1609)
                     /locus_tag="VFC01_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HZN81324.1"
                     /translation="MKLKQVVAGAAIGCALGGAAAVALPSGVANAAPPCNPAPGVQCG
                     PGGPGGPPPQRGPGGPPEDRGPGGPPQGDFRGRGGPPPGDFRGPGGPGGLPPGDFHNP
                     GGPDFRGPNNHDWRPPWNPGDNDWRGRFHGAPWGNDLPPWGWGAPPAPPWDGPLPDPW
                     GPPPAPINYWGFQEQPIWDPGFNQWGFWFFGIWIPLPI"
     gene            complement(1752..3044)
                     /locus_tag="VFC01_16790"
     CDS             complement(1752..3044)
                     /locus_tag="VFC01_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN81325.1"
                     /translation="MNKDDMILISVDDHIVEPPDMFRNHLSKKYLDEAPRLVHNPDGS
                     DTWQFRDVVIPNVALNAVAGRPKEEYGLEPTGLDEIRPGCYNVDERIKDMNAGGILGS
                     ICFPSFPGFAGRLFVTEDPEFSLALLQAYNDWHVEEWCGAYPARFIPMCLPVIWDAEA
                     CAKEVRRNAERGVHALTFTENPAAMGYPSFHDDYWTPLWEALVDTDTVMNVHIGSSGK
                     LAITAPDAPMDVMITLQPMNIVQAAADLLWSKPIKKYPDLKIALSEGGTGWIPYFLER
                     ADRTYEMHSTWTGQDFKGKKPSEVFRDHFLTCFISDHVGVALRNEIGIDNICWEADYP
                     HSDSMWPGAPEQLHDVLTKNNVPDDEVNKMTFENAMRWYHWDPFTHISREQATIGALR
                     KAAEGHDVSIQARSHHKKDTSLGASTLQAQLDRNNAPR"
     gene            3697..3858
                     /locus_tag="VFC01_16795"
     CDS             3697..3858
                     /locus_tag="VFC01_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81326.1"
                     /translation="MQREIDTANAQQRLLATRLRQFAAAYRTVTPEAAIFGIDPFRSA
                     LTGEDFFSR"
     gene            complement(3921..4673)
                     /locus_tag="VFC01_16800"
     CDS             complement(3921..4673)
                     /locus_tag="VFC01_16800"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZN81327.1"
                     /translation="MTTSTTSDLSADWITLTTTDGPMRVYRARPRAGEALDPPAAVIV
                     LQEAFGVNDHIQDVTRRFAEEGYLALAPDLFHRSGKKTVDYSDFDTAMQLIGELGREP
                     IISDVAAVLATLADDDGIPADRIAVVGFCFGGRAAFTAATALPVAATVVFYGPGIASG
                     PHAVVDQTAQITGPVLLLVGDADPTIPSADLAAIEAAACQSNVKLRSVVFAGAGHAFH
                     CDARPDQYVADAAGKAWQDTTEFLAQTITRRT"
     gene            complement(5574..6326)
                     /locus_tag="VFC01_16805"
     CDS             complement(5574..6326)
                     /locus_tag="VFC01_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81328.1"
                     /translation="MTQTATSVGPANQHARRIPLPTGHREPMKVNVFNFITGATCQLL
                     PLFPYDDAGAMVPCGAVFTGDPKDSEFGHFFHYNTVQEVAVTFGANNGMLQSGQIFVT
                     QQLHGVNSFLRDTTDPEAFILMTITQHQSEDGDQNEAILFRCQKCHEELLRHEYNATP
                     KGVDGYDPSQWGGSMTDEVPMFTTIWGTNKAAVDYDDESVRTCPKCGHLNAPHPHHKA
                     GWQRYLNQVRAAESAKRALRSAAQSATESTAV"
     gene            6961..7083
                     /locus_tag="VFC01_16810"
     CDS             6961..7083
                     /locus_tag="VFC01_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81329.1"
                     /translation="MQIPVTLSLTAGPAFRNGLEVVVGYLTPVIRWSILYLAES"
     gene            7126..8571
                     /locus_tag="VFC01_16815"
     CDS             7126..8571
                     /locus_tag="VFC01_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZN81330.1"
                     /translation="MTDHEAVFEAVRRGMIPAYIYNDSKLFALECDRIFSRAWVLVGH
                     ESEIPQEGDYVVRRVLNDSFIITRDSKGDIRAMFNMCLHRGMQVCRAEMGNASNFRCP
                     YHGWSYRNDGRIIGLPFHGEAYGGEHGFKKRGQTLLPAPNLASYNGLIFISMDPGAEP
                     LDQYLGDFTFYLDFYTRQSKAGLEVRGPQRWRIKANWKIGPENFAGDMYHTPQTHTSV
                     VEIGLFREPKAEKRKDGATYWADKGGGTTYKLPPGDFRERMRYVGYTDDMIDRIKEVW
                     TPDQQRLVGDDGFMISAASCFPNMSMVHNWPKVEDGENVLPFISFRMWQPISEHETEV
                     YSWFAVDAEAPEEYKKNSYKAYLMCFGSTGMFEQDDVENWVSLTNTAAGSMARRLQLN
                     SRMGLLEDDTPVCKHLGPQEFHGPGVAQIGYNEANQRALLRLWADYLEQPAVSTQAMR
                     LGTGMDGIPPLIQTSCVDASTMTADRQVTRA"
     gene            8568..9191
                     /locus_tag="VFC01_16820"
     CDS             8568..9191
                     /locus_tag="VFC01_16820"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HZN81331.1"
                     /translation="MSTAGTDRVVGFRRAGRGGTYSEQAVLGGHAPRDHRVGDSLPFD
                     DERHLVAHRFLVDEAYLLDAQAYEEWLELLTDDVHYVMPVRVTTALGAGFDSSPGMAH
                     FDENKYSLSRRVARFLTEHAWTEDPPSRLRHYVTNVRTFATEKDDHLIVESAELLFRS
                     RGDVNEAALVSCGREDLLRFEDRWKVARRTIFIDESVIRMQNLAIFL"
     gene            9188..9508
                     /locus_tag="VFC01_16825"
     CDS             9188..9508
                     /locus_tag="VFC01_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodiol dehydrogenase"
                     /protein_id="HZN81332.1"
                     /translation="MREDLHRGGDHEDAASRATELDKLLDSVVGQPITVANEFTSTEI
                     RRIDTRNGSRLLVTAPRTGQWISLDALEVEALTRQNTATLAAMVGSMHAPLIPDGPDG
                     EEQL"
     gene            9505..10356
                     /gene="hcaB"
                     /locus_tag="VFC01_16830"
     CDS             9505..10356
                     /gene="hcaB"
                     /locus_tag="VFC01_16830"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(cis-5,6-dihydroxycyclohexa-1,
                     3-dien-1-yl)propanoate dehydrogenase"
                     /protein_id="HZN81333.1"
                     /translation="MSGSLEGKRALIVGAGSGIGRAVVDAFRDEGARVAVLERDLHKC
                     AVLRQEAGDVPVVAGDATTREANDDAVSTAVQAFGGLDTLVNCVGVFDFYRGLSEIAA
                     EDLERAFVEMFRVNVLSHLQSVKAAIPALQQGRGSAIVLAESASSFYPGRGGALYVSS
                     KFAVRGLVATLAYELAPRIRVNGVAPGGTVNTDLRGLESLGQSSIRLDDTADRARSLA
                     ARTPLNVALSGDDHAWSFVYLASDRARGITGDTLHPDGGFSLGPSRSRPADKQAATYP
                     QSSAEVV"
     gene            10359..10553
                     /locus_tag="VFC01_16835"
     CDS             10359..10553
                     /locus_tag="VFC01_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN81334.1"
                     /translation="MARINADVRLCQGYANCVAAADDYFDIDDDGRVVLLHSDVPETD
                     YSRVAQAAHSCPVSVLSVER"
     gene            10550..11806
                     /locus_tag="VFC01_16840"
     CDS             10550..11806
                     /locus_tag="VFC01_16840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006897054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HZN81335.1"
                     /translation="MSSSTAGVVIVGASVAGVRTAQALRREGFDGRVVLLSDEAVMPY
                     DKPPLSKGFLAGAAGPDQIALLTEVEADELGVEVRLNSRARRLHLAEHLVELDTGEHV
                     AYHRLVVATGASARESTWPVSGVHVVRTMSDAQRLRADLERGGHLVVIGAGFVGSEVA
                     ATARMMGLQVTLVDPNTAPMTRIAGNQLGARFIRLHQDHGVDTRFGTSVLDVSRKYGR
                     LHIDLSDGAALTADTCVVGIGAVPNDAWLASSGVRIDNGVLCDEYCRAIGNPDVYAVG
                     DVARWHNPRRGLLTRHEHWTNAHEQANTVAHNIIHAQNLIAFAPIEYVWSDQYDWKIR
                     IAGQIGVGTCDIVEVIGDEATERFAVLSSCDGASLSGIVAVNWPRALIAGRKALAHNM
                     SYTDLRQAIEQLLSAMPTPVAMEGAK"
     gene            11803..12537
                     /locus_tag="VFC01_16845"
     CDS             11803..12537
                     /locus_tag="VFC01_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016885006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZN81336.1"
                     /translation="MSFTDELDRQRELVARACRIAAGRGLVDGVLGHISLRITEETFL
                     VRCRSDDDTGVEFTRPSDIRLVRFDGSAGAAGELDGYRVPNELPIHVECLAARPQVRA
                     VAHLHPANIVAADLAGIAMRPICGAFDIPAAFLARGGVPVYPRAVLIRNRELAGAMVS
                     AMGDRPLVICRGHGIVSTAESVQQAVLQAIGLDEMARMALRVLAAGGVLHDIDDSDWD
                     DLPDLGPSFNADAAWRYEVARLDART"
     gene            12625..13422
                     /locus_tag="VFC01_16850"
     CDS             12625..13422
                     /locus_tag="VFC01_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN81337.1"
                     /translation="MKKTAVTDVAASGPTPQYPIESVDNALKVLLLLAERTELRLTEV
                     SEYLGVASSSAHRMLAMLVYRGFVRQDPKTKAYRAGAALTTVAFSILNRFDFRVTLHP
                     YLEKLNLELGETVHLTMLDGASVCFVDAIEGSGVVRTASRVGVSLPASTTASGKAMLA
                     ALSLAELDSLYPAEELVTLTDRSVATRSELYRQLEQIRRRGYSWSSEESEAGVSSVAV
                     AFPFRENLPRLAFSTAVPRPRMAHADKRRISETLMGIVADAADALHP"
     gene            complement(13443..14837)
                     /locus_tag="VFC01_16855"
     CDS             complement(13443..14837)
                     /locus_tag="VFC01_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011593909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZN81338.1"
                     /translation="MTTLNPSNGQALHTYPVTTRSELSAALHTAATIQREWSRTDLAH
                     RAQALRNTAAQLSQNRDRLALLVTREMGKPIREALAEVDKCAAVLDYYAERGERFLAD
                     EINDDTDADHSWVSFDPLGVILGIMPWNYPLWQVFRFAAPALMAGNAVLLKHAANTTG
                     CALAIQRVLIDAGLPPGVLTALVIAEDDVPSATAQLVADPRIAAVTLTGSERAGSAVA
                     AIAGREIKKCLLELGGSDPFIVLADADLERVAAHAVRARFLNAGQSCISPKRFIVEQP
                     VATQFTRLVVEAAERLKTGDPEKPDTDIGPLARRDLRDAFARQVRSSVEAGAVVLAGG
                     CAPSDCAGNYYLPTVLGGVRPGMPAYDEEMFGPAASIITVNDVDHAVAIANDTPYGLG
                     ASVWSSDLSIARSVGQQITSGACFVNAVVTSDPRVPFGGTKRSGYGRELGRAGIREFV
                     NARTWWVMDTAREL"
     gene            complement(14884..>15322)
                     /locus_tag="VFC01_16860"
     CDS             complement(14884..>15322)
                     /locus_tag="VFC01_16860"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN81339.1"
                     /translation="QPEALAAFIDYTDATKKALPDDLAEVVALTVATLTGNDYERHQH
                     ERLSIKLGLTKDWVESIERLAPESTALTLEQRATQRWVLAVLPNHGHGSESEVDELVR
                     TLGPRDAIAVALMCGRYVAHATIANSFGIQPPVASIFSEQTTL"
BASE COUNT         2756 a         4666 c         4997 g         2903 t
ORIGIN      
        1 acgctcattc acatggacga tccgcaacac cgcgtggtca gggcgatcgg tgcggactgg
       61 tttcggccga aggccatgcg cgccctgaaa gttcgtgtcg acgaactcgc gaagcggttc
      121 gttgaccaga tgctcgcggc gggaccggag tgtgacttcg tccagcaggt cgcggtgaac
      181 tacccgctct acgtgatcat gtcgatgctc gggctgcccg aagaggactt cccgcgcatg
      241 ctcaagtaca cccaggagat gttcggcaac gacgaccccg aattgcagcg cggcacaacc
      301 aaagaggagc agatggcgtc gctgctcgat atgttcgcat acttcactgc ggtgacggcc
      361 tcccgtcggg agaacccgac cgaagatctg gcgtcagcca tcgccaacgc cacgatcgat
      421 gatgagccgc tatccgacat cgagacggtg tcgtactact cgatcgtcgc cgccgcgggt
      481 cacgacacca cgagcgcggt gatctccgga gggttgcacg ccctgctcga gcaccccgat
      541 cagcttgcgc gacttcagga caacatggac ctgatgccgt tggccaccga ggaaatgatc
      601 cgctgggtca cgccggtcaa ggagttcatg cgcaccgcgc aggcggacac caccgtccgc
      661 ggtgtgccga tcgccgccgg ggaatcggtg ttgctgtcct atgcgtcggc caaccgcgac
      721 gaagacgtct tcgacgaccc gttccggttc gacgtcggcc gcgatcccaa caagcacaac
      781 gcgttcggct acggcgtgca cttctgtctc ggcgctgcgc tcgcgcgaat ggaagtcaac
      841 agcttcttct cagagctggt cccgctgctg gagtcgatcg aactggccgg cgaccctgcg
      901 cacatcgcca cgatcttcgt cggtgggctc aagcacctgc cgatccgcta ctcgctcaag
      961 tgaatcaaga ggacggccgc cccaggggac gaggcggccg tcctgcgcca gatcagatcg
     1021 gaagcggaat ccagattccg aagaaccaga aaccccattg gttgaagccg ggatcccaga
     1081 ttggctgctc ctggaagccc cagtagttga tcggtgccgg cggcggcccc cacggatctg
     1141 gcaacggacc atcccacggc ggcgccggcg gtgcgcccca gccccacggc ggcaggtcgt
     1201 taccccacgg cgcaccgtgg aaccgtccgc gccagtcgtt gtcacccggg ttccacggcg
     1261 gtcgccagtc gtggttgttg ggtccgcgga aatcggggcc gccagggttg tggaagtcgc
     1321 cgggtggcag accgcctggt ccgccaggtc cacggaagtc gccgggcggc ggaccgcccc
     1381 gtccgcggaa gtcaccctgc ggcggacccc cggggccgcg gtcttcgggt ggaccaccgg
     1441 gcccgcgctg tggcggagga ccgcccggac cacctgggcc acactgcacg cccggtgccg
     1501 ggttgcacgg aggtgccgcg ttcgcgacac ctgacggtag tgcgacggcc gccgcgccac
     1561 ccagagcgca gccgatcgcg gctcccgcga ccacttgttt gagcttcata acctcgcctt
     1621 tcgcgcgtcg gtagacgccc caacccacac gcgaggctag gtaggcaggc taagagcgag
     1681 ctatgtgctc agtagcaatg agctgggcgt gctggatcgc cgctgttaat tgacaaggcg
     1741 gtcaacaaga ttcaacgcgg ggcattgttc ctgtccagtt gcgcctgcag cgtgctcgcc
     1801 ccgaggctcg tgtccttctt gtggtggctg cgcgcctgga tggagacgtc gtggccttcg
     1861 gcggccttgc gcagcgcgcc gatggtggcc tgttcgcggc tgatgtgggt gaacgggtcc
     1921 cagtgatacc agcgcatggc gttttcgaag gtcatcttgt tgacctcgtc atcgggcacg
     1981 ttgttcttgg tgagcacgtc gtggagttgc tccggcgcgc cgggccacat gctgtcgctg
     2041 tgcgggtagt cggcctccca gcagatgttg tcgatgccga tctcgttgcg tagcgccacc
     2101 ccgacgtgat cggagatgaa acaggtcaag aagtggtcgc ggaacacctc ggagggtttc
     2161 ttgcccttga agtcctgacc ggtccaggtc gaatgcatct cataggtgcg atcggcgcgc
     2221 tccaagaagt acggaatcca gccggtcccg ccttcggaga gcgcgatctt gagatccggg
     2281 tacttcttga tcggcttgga ccacaacaga tccgccgcag cctgcacgat gttcatcggc
     2341 tgcagggtga tcatcacgtc catcggcgca tccggggcgg tgatcgcaag ctttcccgag
     2401 gagccgatgt gcacattcat cacggtgtcg gtatcgacga gtgcctccca cagcggtgtc
     2461 cagtagtcgt cgtggaagga cggataaccc atggccgcag ggttctcggt gaacgtcagg
     2521 gcgtgcacac cacgctcggc gttgcgccgc acctctttgg cacaggcctc ggcatcccag
     2581 atcaccggca gacacatcgg aatgaagcgc gccggatagg ccccgcacca ctcctcgaca
     2641 tgccagtcgt tgtacgcctg cagaagagcg agggaaaact cgggatcttc cgtcacaaac
     2701 aaccgaccgg caaaaccagg aaatgacggg aagcaaatcg agcccaggat gccgccggcg
     2761 ttcatgtcct tgatgcgctc gtcgacgttg tagcagcccg gccggatctc atcgaggcct
     2821 gtcggctcca ggccgtactc ctctttcggt cgacccgcca ccgcgttcaa cgcgacgttg
     2881 ggaatgacca catcgcggaa ctgccacgta tccgacccat cagggttgtg caccaaccgc
     2941 ggcgcctcat ccaggtactt cttcgacagg tgattgcgaa acatgtccgg cggttcgacg
     3001 atgtggtcgt cgacgctgat caggatcatg tcgtccttgt tcacaggacg ctcccttcag
     3061 aagatgcgct taccgtatgg ccaacagttt agcgatacga tccccgtacc caacaaccta
     3121 cggcgacgcg aggtgatcat gtcgaacttc tccgctccag gtcgccctga acatgtgtag
     3181 ttggcggtgt cgccgacggc ggtgatcgat gccccgaccc ccggtggcca tgtgtagtac
     3241 tccagcttcc gtcgggtcac gtcgtggtga agcgagtcac ctgacctcgc cgactcgacc
     3301 cgagacacct gcgcttgggt gtggttgtga ccacagccgc tggttaacgt tgattggtga
     3361 ccggctcagt gagtgcctat gtcgcgcacg tcgacgactt gcttgctgcc ggacaggcgc
     3421 tgttcggaca gtccaccggc ccaacaagtc tgagccctac tgacctacgg tcgataccgc
     3481 caccgccgca aaagcggagt cagcctcagc atggatgccg ccgctgacga ctatcaatag
     3541 aactggcgca cagttaccaa agctagatgc ccacacggat agtcagcgca gcgccagtcg
     3601 ttccgagaac gagcgcacgg gtggggcagc gacggacgtg cgggttgcgg ccggagtgca
     3661 gcggctggtg tcgatgatgg acgaagcctc gattcgatgc aacgcgagat cgacaccgcc
     3721 aacgcgcagc agcgactgct agccactcgg ctgcgccaat tcgccgccgc ctatcggacg
     3781 gtcacccccg aagctgcaat attcggaatc gaccccttca gaagcgcgct taccggtgaa
     3841 gacttcttct ctcggtgagc gcggatagcc gtaggtggca aggaatctca ggtagtcgga
     3901 tggccgcacc gcggtgcggt tcacgtccgt cgcgtgatgg tctgggccaa gaactctgta
     3961 gtgtcctgcc atgccttgcc tgcggcgtca gccacgtact ggtccggtcg cgcatcgcag
     4021 tggaaagcgt gcccggcccc ggcgaagacc accgagcgca gcttgacatt tgattgacag
     4081 gcggccgctt cgatcgcggc gagatccgcg gaaggaatcg tgggatcggc atcaccgaca
     4141 agcagcagta ctgggccagt gatctgggcg gtttggtcca cgaccgcgtg ggggccgcta
     4201 gcgatacctg gcccgtagaa gacgaccgtg gcggcgacag gaagcgcggt agccgccgtg
     4261 aacgctgcgc ggccaccgaa gcagaacccg acgaccgcga tgcgatcggc agggattccg
     4321 tcatcgtcgg cgagggtggc cagcaccgcg gcgacgtcgg agatgatcgg ttcgcgaccg
     4381 agttcaccga tcagctgcat cgcggtgtca aagtcggagt aatcgactgt cttctttccg
     4441 gaacggtgaa agaggtccgg ggcgagcgct aggtagccct cctcggcgaa ccggcgagtc
     4501 acgtcctgaa tgtggtcgtt gacgccgaaa gcctcctgca acacgatgac cgcagccggt
     4561 ggatccagcg cctcgccggc gcgcggacgc gcccggtaga cccgcatcgg cccgtcggtg
     4621 gtggtcagcg tgatccagtc ggcgctgaga tcgctggtgg ttgaagtggt catggccggt
     4681 gtttcctttc atcgatggtg cacacagaca tcaattccct gtcgggctcg aaatgacggt
     4741 gatactcggg atatgcgccg tcggcgtcga cggcgactgg gtgaaacgcg atgggcgctt
     4801 acgcgatcgc ttgcggtgct tgtggctacc ccagacgacg acgctcgcat tgtgtttgag
     4861 tgactacggg cggtgtcgaa cgcctgttgt cgcggggcgg cacgggtcgc gtgagtgcga
     4921 caggagagtg tgatcggagc attgatggtg gattggtcac gaaaccgggc cgcattggat
     4981 cagaacgggt tttagcactg ctcgctggtc acgaggtgag caactcgtcg cgcaggtgct
     5041 gcgcgagcgg cagccagcag ctgaaaggtt cgaggtcgat gcgtgggtgt tgggcgccgc
     5101 agtcggggca gtgacgtagc gactcgtcgt cgttgaaggc gacacatgca tcgaaataga
     5161 cttgctgaga gactgtttca gcggtgtccc actcgctgcg gtacaactcg cggtcgcatc
     5221 cggggcagta ccaggcaacg ccctccagcc cagcagaccg cgctctatat cgcagctgca
     5281 cgccttcctc ggacggcacg acgcggtggg ggttccggcg ggcacgtaca cgtggtcgcc
     5341 gaccgccata tggaaccggc gaaccgagga ctccttcaga tgcatgtccg cggtccccga
     5401 catctgcacg acgacagtgt ctttgccgca aatcagatag aacggctgcg ccacggcgtt
     5461 gcgcgacatg tagagctgcg ggtcgacgtc ggccggaagc accggtacgt cctcatagtt
     5521 tccgacttct tgtgccgctt tgaacgtgtg cagcatcctg cgtctgtcca tgttcacacc
     5581 gccgttgatt ccgtagccga ctgtgccgct gagcgcaatg cccgtttcgc ggactccgct
     5641 gcgcgtacct ggttgaggta gcgctgccag cctgctttgt ggtgtggatg cggggcattg
     5701 agatggccac atttgggaca ggtgcggacg gactcgtcgt catagtcgac cgcagctttg
     5761 ttggtgcccc atatcgtggt gaacatcggg acttcgtcgg tcatcgagcc gccccattgc
     5821 gacgggtcgt agccgtcaac gcctttgggc gtcgcgttgt attcatggcg gagcagttct
     5881 tcgtggcatt tctggcatcg gaacaagatc gcctcgtttt ggtccccgtc ctccgactga
     5941 tgttgggtga tcgtcatcag gatgaatgcc tcggggtcgg tggtgtctcg caagaaggaa
     6001 ttcaccccgt gcagctgttg ggtgacgaag atctgaccgc tttgtagcat cccgttgttg
     6061 gcaccgaagg tgaccgcaac ttcttggaca gtgttgtagt gaaagaaatg gccgaactcg
     6121 ctgtccttgg gatcgccggt gaagactgca ccgcacggca ccatcgctcc ggcatcgtcg
     6181 tagggaaaga gcggcaacag ctgacacgtc gcaccggtga tgaagttgaa cacgttgacc
     6241 ttcatcggtt cacgatgccc ggtcggcagg ggaatgcgtc gcgcgtgttg attcgccggg
     6301 ccgacgcttg tggcggtctg agtcatgctc gtctccttcg tgtggtgctg tcatagctcg
     6361 gccgcggtgg tgaggacgcc gtcgcagaca gccgttgcat gctggtatgg ctgtactggg
     6421 gaacccgctg ctcggtacga gaccacccct gcggtaccgc cgacagcggc ggtgaagccg
     6481 tcccatcgat gacgcttgtg gcagtggggc cgacgctgac tcccgagtta cggcaacagg
     6541 tcgccccggg taatggcact cccagtcgac cgtgacatcg gcagcggctg gtgctgccag
     6601 cacgtaattc accatggcga attcagtgtc atccagatca ccccacgctg tcaagaaatt
     6661 cgtcatgatc gttcatgctg aacctgtagc gggccggggc gggtgatgtg ggtgacgcga
     6721 gcgaaacagc aggcggtgcg gtgcgacccc gagcacgtcg ttgcggtggg taagcggcga
     6781 tgtgacggtg gtgaggttga gccaggtcgc cgaacttgcg gatcgggaat cgatcaatcc
     6841 gcatgattac tatcgttgtt gcacagggct ttacagctcc cagccggact cgccggtatc
     6901 tcgacacggt caccgcacgg tggcggcggc cgacaggatc agatcggcac tcaggccagc
     6961 atgcagatcc cggtcacgtt gtcgctgaca gccggtccgg cattccgcaa cgggctggag
     7021 gtcgtcgtcg gctacttgac tccagtcatc cggtggagca ttctctatct agcggaatca
     7081 taattctgtc tgtcagagat tcggtgatgc gcactggagg gttcgatgac ggatcacgag
     7141 gctgtgttcg aagccgtgcg gcgcggcatg attcctgcct acatctacaa cgacagcaag
     7201 ctcttcgccc tggaatgcga tcggattttc agccgcgcat gggtgcttgt cggccacgag
     7261 tcagagattc cccaggaggg cgactacgtg gtgcgacggg ttctcaatga ctcgttcatc
     7321 atcacgcgcg actccaaggg cgatatcaga gcgatgttca acatgtgcct gcaccgtggc
     7381 atgcaggtgt gccgggcgga gatgggcaat gcgtcgaact tccggtgccc ctaccacggc
     7441 tggtcttacc gcaacgatgg ccggatcatc ggtctgccgt ttcacggcga ggcctatggc
     7501 ggcgaacacg gcttcaagaa gcgcggccaa acgttgttgc ctgcaccgaa tctcgccagc
     7561 tacaacggct tgatcttcat cagcatggac ccgggtgccg agcctttgga ccagtatctc
     7621 ggcgacttca cgttctatct ggacttctac accaggcaga gcaaggccgg ccttgaggtc
     7681 cggggtccgc aacggtggcg catcaaggcc aactggaaga tcggtcccga gaacttcgcc
     7741 ggcgacatgt accacactcc ccagacacat acgtcggtgg tggagatcgg cctatttcgc
     7801 gagcccaagg cggaaaagcg caaggacggc gcaacgtact gggccgacaa gggcggcggc
     7861 acgacctaca aactgccccc cggcgacttc cgcgagcgga tgcgctacgt cggctacacc
     7921 gatgacatga tcgaccgcat aaaggaggtc tggactccgg atcaacagcg tctcgttggc
     7981 gatgacgggt tcatgatctc ggccgcatcg tgctttccga acatgagcat ggtgcacaac
     8041 tggccgaagg tcgaggatgg cgagaacgtc ttgccgttca tatcttttcg gatgtggcaa
     8101 ccgatcagcg aacacgagac cgaggtctac tcgtggtttg cggttgacgc tgaagcaccc
     8161 gaggaataca agaagaactc ctacaaggca tacctgatgt gcttcggctc gaccgggatg
     8221 ttcgagcaag acgacgttga aaactgggtc tcattgacca acaccgccgc cggctcgatg
     8281 gcgcggcgac tgcagctcaa tagccggatg ggtctgctcg aggatgacac acctgtctgt
     8341 aagcacttgg ggccacaaga gttccatgga ccgggggttg cacagatcgg ctacaacgag
     8401 gccaatcagc gtgcgctgct acgactgtgg gcggactatc tcgagcaacc agccgtctcg
     8461 actcaggcca tgcgactggg caccggcatg gacggcatcc cgccgctgat ccagaccagc
     8521 tgcgtcgacg cgtcgacgat gaccgcagac cgacaggtca ctcgggcatg agcacggcgg
     8581 gaacagacag ggtcgtcggt tttcggcgag ccggacgtgg gggcacctac tcggagcagg
     8641 cagtcctagg tggccacgct cctcgcgatc accgtgtcgg tgacagcttg ccgttcgatg
     8701 acgaacgtca cctagtggcg caccgattcc tcgtcgacga ggcgtatttg ctggatgccc
     8761 aggcttacga agagtggctc gaactgctta ccgacgatgt gcactacgtc atgccggtac
     8821 gggtgacgac cgccctcggc gctgggttcg attcttcgcc cgggatggcg catttcgacg
     8881 agaacaaata ctcgttgagc cgcagggtgg cgcgtttcct gactgagcac gcctggaccg
     8941 aggacccgcc atctcggctg cgtcattacg tcaccaacgt gcgtacgttt gccactgaaa
     9001 aagacgacca tttgatcgtt gagtcggccg aactcttgtt ccgcagtcgc ggcgacgtca
     9061 acgaagcggc cttggtgtcg tgtggtcgcg aagacctgct gcgcttcgag gaccgctgga
     9121 aggtagcgcg gcgcaccatc ttcatcgacg agtccgtcat ccgtatgcag aatctggcga
     9181 tcttcctatg agggaggact tgcaccgagg cggcgaccac gaggatgcag cctcgcgggc
     9241 gacagaactg gacaagctgc ttgattcggt tgttgggcag ccgatcaccg tcgccaacga
     9301 gttcacctcg accgagatcc gccgaatcga cacccgcaat gggtcgcggc tgctcgtcac
     9361 ggcgccccgc acgggacagt ggatcagcct ggacgccctc gaagtcgagg cgttgacgcg
     9421 gcagaacacc gcgacgctgg cggcgatggt cggcagcatg cacgcaccgt tgatcccgga
     9481 tggaccggat ggagaggagc agctgtgagc ggctcactcg agggcaagcg cgctttgatc
     9541 gtcggcgccg gctcgggcat cggccgtgcc gtcgtcgacg cgttccgtga cgagggcgct
     9601 cgggttgcag tcttggagcg cgacctgcac aagtgcgcgg tcctgcgcca agaggccggt
     9661 gatgtgccgg ttgtcgcggg cgacgccacg accagggaag ccaatgacga cgccgtctcg
     9721 acggcggtgc aggcattcgg cgggttggac actttggtga actgcgtggg agtgttcgac
     9781 ttctaccgag ggctgagtga gatcgccgcc gaggatctcg agcgtgcttt cgtcgagatg
     9841 ttccgcgtca acgtgctcag ccacctgcag tcggtcaagg cggccatccc ggcgctgcag
     9901 cagggtcgtg gatcggccat tgtgctggcc gagtcagcct cctcgtttta tccgggtcgt
     9961 ggtggcgcgc tttatgtttc gtcgaagttc gcggtacgcg ggttggtcgc gacgctcgct
    10021 tacgaactgg ctccgcgcat ccgggtcaac ggggtggccc ctggcggtac ggtcaatacc
    10081 gatctgcgcg gtctggagtc tttgggccag agctcaattc gtttggacga caccgccgat
    10141 cgtgcccgca gcctcgcggc gcgcacaccg ttgaatgtgg ccttgtcggg cgatgaccat
    10201 gcgtggagct tcgtgtacct agcgtcggac cgcgcacgcg gcatcaccgg tgacacgctt
    10261 cacccggacg ggggcttcag cttgggcccg agtcggtcac gccctgccga taagcaggcc
    10321 gcgacgtacc cgcaatcttc agcggaggtg gtatgagcat ggcgaggatc aacgccgacg
    10381 tgcggctgtg ccagggatac gcgaattgtg ttgcagcggc tgacgactac ttcgatatcg
    10441 acgacgacgg acgcgtcgtc ctcctacata gcgacgtgcc tgagactgac tacagccgcg
    10501 tagcgcaggc tgcccacagt tgcccggtgt cggttttgag cgtcgagcga tgagcagcag
    10561 taccgccggc gtcgtcattg tcggcgcctc agttgctggt gtgcgcacag cgcaggccct
    10621 gcggcgcgaa ggattcgatg gacgtgtcgt gttgttgtcc gacgaagcag taatgcccta
    10681 cgacaagccg ccgctgtcga aggggtttct cgccggtgcg gccgggcctg atcagatcgc
    10741 gttgttaacc gaagtcgagg ctgacgaact gggcgtcgaa gtgcgtttga actctcgtgc
    10801 gcgccgcctc catttggcgg agcacctggt cgagctcgat accggcgagc acgtcgccta
    10861 tcaccgtttg gttgtcgcga cgggagcgag cgccagggag tcgacatggc cagtttcggg
    10921 agtgcacgtg gtgcgcacca tgtccgacgc gcagcgactg cgcgccgacc ttgagcgcgg
    10981 cgggcatctg gtggtgatcg gtgcggggtt cgtggggtcc gaggtcgccg ccacagctcg
    11041 catgatggga ctgcaggtca cactcgtcga ccccaacacg gcgccgatga cgaggatcgc
    11101 cgggaaccag ctgggtgccc ggttcatccg tctgcatcaa gaccacggcg tggacactcg
    11161 attcggcacc agcgtcctcg acgtcagccg aaagtacggg cggctgcaca tcgacctcag
    11221 cgatggcgcg gccttgaccg ccgacacgtg tgtggtcggc attggcgcag ttcccaacga
    11281 tgcatggttg gcgtcgtcgg gcgtgcggat cgacaacggc gtcctctgtg atgagtattg
    11341 ccgcgcgatc ggtaaccccg atgtgtatgc ggtcggtgac gtggcccgct ggcataaccc
    11401 ccgtcggggc ctgctgaccc gtcacgaaca ctggaccaat gctcatgagc aggccaacac
    11461 ggtggcgcac aacattattc acgcccaaaa cctgatcgcc ttcgccccga tcgagtatgt
    11521 gtggagtgat caatacgact ggaagatccg cattgccggg cagattggtg tcggcacctg
    11581 cgacatcgtc gaggtcatcg gtgacgaggc gactgagcgt ttcgctgtgc tgagcagctg
    11641 cgatggagca tcgttgtcgg ggatcgttgc cgtcaactgg ccgcgcgcac tcatcgccgg
    11701 ccgtaaagcc ctggcccaca acatgtccta caccgacctc aggcaggcga tcgagcaact
    11761 gctgagcgcg atgcccaccc ccgtagctat ggaaggtgca aagtgagctt caccgacgag
    11821 cttgaccggc agcgagaact ggtggcacgc gcatgccgga tagcggctgg ccgcggtctg
    11881 gtggacggcg tcctcggaca tatcagcctg cggatcaccg aagagacgtt cctggtccgt
    11941 tgccgcagcg acgacgacac tggcgtcgag ttcaccaggc caagcgacat cagacttgtc
    12001 cgattcgatg gatcagccgg tgccgcaggt gaattggacg gctatcgtgt ccccaacgag
    12061 ctgcctattc atgtcgagtg cctagctgcg cggccgcagg tgcgcgcggt ggctcacttg
    12121 catccggcca acattgtcgc cgctgacctc gccggcatcg cgatgcgacc catctgcggg
    12181 gcgttcgaca ttccggcggc gtttttggcg cgcggaggcg tgcccgtgta cccgcgggcg
    12241 gtgttgatcc gcaaccgcga actggccggg gcgatggtgt cggcgatggg tgatcgaccc
    12301 ttggttatct gccgcgggca cggcatcgtc agcaccgcgg aatcggtgca acaggcggta
    12361 cttcaggcga tcggcctcga tgagatggca cgtatggcgc tacgtgtcct cgccgcgggt
    12421 ggggtgttgc atgacatcga cgacagcgac tgggacgatc tgcccgacct gggacccagc
    12481 ttcaacgccg atgcggcctg gcgttacgag gtggcacggc tcgacgcacg cacgtgacca
    12541 gagccaaaag tggaagtccg tgcattgcag ggtccgagtg cggcgtcgtg gctacgcccc
    12601 tcggccgtcg ctactatctc tgctgtgaag aaaactgcgg tcacggatgt cgcggcaagc
    12661 ggaccgacgc cgcaatatcc gatcgaatcg gtggataacg ccctaaaggt cctgctgctt
    12721 ctcgccgaac gcaccgagtt gaggctgacc gaggtcagcg agtacctcgg ggtggcgtcg
    12781 tcgagtgctc accgaatgtt ggcgatgctg gtctaccgcg gcttcgtccg acaggacccc
    12841 aagacgaagg cgtaccgtgc cggggccgca ttgaccaccg tcgccttctc catcctgaat
    12901 cgattcgatt tccgtgtgac attgcaccca tatctggaga agctcaacct ggagctgggc
    12961 gagacggtgc acctcaccat gctcgacggc gcgtcggtct gcttcgtcga tgcgattgaa
    13021 ggctcggggg tggtgcggac cgcgtcccgc gtcggcgtgt cactgccggc aagcacgacc
    13081 gcatccggaa aggcgatgct ggccgccctg tcactcgccg aactcgacag cctttatccc
    13141 gcagaagaac tcgtgaccct caccgatcga agtgtcgcga cccgaagcga gctgtatcgc
    13201 cagctggagc agatccgccg ccgtggctac tcgtggagct cggaggagag cgaagccggt
    13261 gtctcgtcgg ttgctgtggc gtttcccttc cgcgagaacc tgccgaggct ggcctttagc
    13321 accgcagtgc cgcgtccgcg gatggcccat gcggacaagc gccgcatcag cgagactctg
    13381 atgggtatcg tcgccgatgc tgccgatgct ctgcacccgt aatcgaaaca ccggccgcga
    13441 ggtcacagtt cgcgtgccgt gtccattacc caccatgtcc tggcgttgac gaactcacga
    13501 atacccgctc ggccgagctc tcggccgtag ccgctgcgtt tggtaccgcc gaacggtact
    13561 cgaggatcgg atgtcacaac cgcgttgacg aaacatgctc cggaggtgat ctgttgtccc
    13621 accgatcggg cgatgctgag gtcagaactc cacacgctgg cgcccaggcc gtagggggtg
    13681 tcgttggcga tggcgacggc gtggtcgaca tcgttgacgg tgatgatgga tgctgccgga
    13741 ccgaacatct cttcatcgta ggcgggcatt cccggccgta cgccaccgag aaccgttggc
    13801 aggtagtagt tcccagcaca gtccgaaggt gcacatccgc cagccaagac aacggcaccc
    13861 gcctcgaccg aggaccgcac ctggcgtgcg aacgcatcgc ggagatcccg gcgggcgagg
    13921 ggtccgatgt cagtgtcggg cttttcggga tcaccggtct tgagtcgctc cgcggcctca
    13981 acgaccagcc gcgtgaactg cgtcgcaacg ggttgttcga cgatgaaccg cttgggcgaa
    14041 atgcagctct gtcccgcgtt gaggaaccgt gcccggacgg cgtgggctgc gacgcgctcg
    14101 agatcggcgt cggccaacac gatgaatggg tcggaacctc cgagttcaag gaggcacttc
    14161 ttgatctctc ggcccgcaat tgccgccacg gcgctcccgg cacgttcact gcccgtcagg
    14221 gtcactgcgg cgattcgcgg gtccgcgacc agttgcgctg ttgccgaggg cacatcgtcc
    14281 tcagcgatga cgagcgcggt gagaacgccg ggcggcagtc cggcgtcgat gagcacgcgc
    14341 tgaatcgcca gggcgcatcc cgtggtgttg gcagcgtgct tgagtaatac ggcattacca
    14401 gccatcaatg ctggggcggc gaaccggaac acctgccaca gcgggtagtt ccacggcatt
    14461 atgcccaata tgacgcccag cggatcgaac gacacccagc tgtggtcggc gtccgtgtcg
    14521 tcattgatct cgtcggcgag gaacctctcc ccgcgctccg cgtagtaatc gaggacggca
    14581 gcgcacttgt ccacctcggc cagggcttct cggatgggct tgcccatctc gcgagtgaca
    14641 agtagtgcca ggcgatcccg gttctgggac agctgcgccg ctgtgtttct caacgcctgc
    14701 gcacggtggg cgaggtcagt tctgctccac tcacgctgaa tcgttgcggc agtatgaagc
    14761 gcagcgctca actcggagcg agttgtcacc ggataggtgt gcagcgcttg accgttggac
    14821 ggattcagcg tcgtcaccgt ggtcgtgatg tcctcttctc agctcagtct tacagaagag
    14881 ctttcagagt gttgtctgct cgctgaagat ggaggcgacc ggcggctgaa tcccgaagct
    14941 gttggcgatg gtggcatgcg cgacgtagcg tccacacatc agcgccaccg caatggcgtc
    15001 gcggggaccc agcgtgcgaa cgagttcatc gacctcggat tcgctgccgt ggccatggtt
    15061 gggaaggacc gccagcaccc agcgttgggt cgcgcgctgt tcgagcgtga gcgcggttga
    15121 ctcgggggcg agtcgttcga tcgattcgac ccaatccttt gtcaagccca gcttgatcga
    15181 gagtcgttcg tgttggtggc gctcgtagtc attgccggtc agggtcgcca cggtcaacgc
    15241 gaccacctca gcgagatcgt cgggcagcgc cttcttggtg gcgtcggtgt agtcgatgaa
    15301 tgcggccaat gcttcgggct gg
//
LOCUS       DASRJA010000380         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1998653,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000380 DASRJA010000000
VERSION     DASRJA010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998653"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VFC01_16865"
     CDS             <1..635
                     /locus_tag="VFC01_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN81340.1"
                     /translation="SYLQDWLAGSSATNWRVDPAQGGLSRAFGDIGVHWCDLMEFVTG
                     HRILRLVASTANAYRERGGEAARTEDGAVVLFDTDKGAAGSLVVSQVTPGRKNRLWFS
                     FDGTQASYSFDQEQPDALWVGGTAENRIVTRGPDTLTSDDSRRLARLPAGHPQGYQDS
                     FTAFVSDAYAVAAGEERDGVPTFADGHRAAVLTAAVIDSAKINSWVEVPV"
     gene            632..1315
                     /locus_tag="VFC01_16870"
     CDS             632..1315
                     /locus_tag="VFC01_16870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HZN81341.1"
                     /translation="MTSQQPDLASTSRFFGDHQRATIEAAMARIIPTDDTPGAVEAGT
                     IDFLDRYLSGIDYIYAKPDGSGFEELSGKRADAWRQRVEIMRERYVSGVRDLDERAHA
                     AHDADFVDLTPEQQDGVLRDLELPDQQQETELAKAEATLYGAPVEPALQQTSAEVDLG
                     FVPLLALHTRQGFYADPLYGGNRNRVGWDVIGFPGPTSLAEVHSGRYSTLDYFADDRE
                     HPIAKEEAS"
     gene            1312..2955
                     /locus_tag="VFC01_16875"
     CDS             1312..2955
                     /locus_tag="VFC01_16875"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZN81342.1"
                     /translation="MTNGAKPDRADICIIGAGASGAAAGKVLTERGMRVVMLERGPWR
                     TRETFGGDELANINRYNLWPDPLLNPRTVRDEVGGEFRTELFCPVPQMVGGGTVHWQG
                     WLPRFTEEDFKLHSVVGDVPGTTLVDWPITYDDLEAYYTQIEWAFGVSGRAGANRYES
                     WRSKDYPCPPLPQSRYAQKFHQGCTELGYNSFPTPQAALSRPFNGRPATVISAFAQQH
                     GDPTGTRSSALNVFVPNAVATGRLDLRPNAYVSEITVDERGRAKSAVYADEDGTTYEQ
                     EANVFILACGAVETARLLLLSKSSRFPNGLANGSDLVGRNVTFHEYSAAVGTFEDPIY
                     AWAGGGYVSASSFEFNGHDDSRGFAGGGHIACAGVGIPLPINWSLPNRPAWGAEAKQI
                     DRDYFNHSMAVAMVVHDLPQHDNRVELDDSVTDAWGLPVAKITIIAHENDLAMGRFLI
                     DRNAEILEAAGATSVQKVYIDRITGNCSHQHGTTRMGNDPETSVLDRWCRSHEVDNLF
                     VVDGSPFPTGTGINPTLTIMANAWRVAEHITNGPHTTGQ"
     gene            3475..3837
                     /locus_tag="VFC01_16880"
     CDS             3475..3837
                     /locus_tag="VFC01_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81343.1"
                     /translation="MSASSAITINQTQQDIAARLPQADPPLSDDDADAEIVYAPAPGD
                     RGTEVRVLLPNSPGGLGQKVAAVMGLDAQRELDDALRRFKQLLETGEIARSDGSPTGT
                     DARAQRSQQPAQPMGAYK"
     gene            3855..>3982
                     /locus_tag="VFC01_16885"
     CDS             3855..>3982
                     /locus_tag="VFC01_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006358178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HZN81344.1"
                     /translation="MRANCWMGRNTVEVRDVPDPVILNPRDAIVKITSTAICGSDL"
BASE COUNT          769 a         1313 c         1283 g          617 t
ORIGIN      
        1 gctcgtacct gcaggactgg ctggcgggta gcagcgcgac gaactggcgt gtcgatcctg
       61 cacagggcgg cttgtcccgc gcgttcggtg acatcggtgt ccattggtgc gacctgatgg
      121 agttcgtgac cgggcaccgg atcctgcgac tggtcgccag cacagccaac gcctatcgcg
      181 agcgaggcgg ggaggcggct cggaccgagg acggtgcggt cgtgctgttc gacaccgaca
      241 agggcgcggc cggatcgctc gtcgtaagcc aggtgacacc cggacggaag aaccggctgt
      301 ggttctcctt cgacggcacc caggcgagct actcgttcga tcaggaacag cccgacgcac
      361 tatgggtcgg cggcaccgcc gaaaaccgga tcgtcacgcg cggtcccgac acgttaacca
      421 gtgacgacag ccgccggttg gcaagactgc ccgctggcca tcctcagggt taccaagaca
      481 gtttcacggc ctttgtctcc gacgcttacg ccgtcgctgc cggcgaggaa cgagacgggg
      541 tcccgacatt cgccgacggt catcgcgctg cggtcctcac cgcagcggtg atcgactcgg
      601 ccaagatcaa cagttgggtg gaggtgcccg tatgacgagc cagcaacctg acctggcgtc
      661 caccagcagg ttcttcggtg atcaccaacg cgccaccatc gaggcggcga tggcgcgcat
      721 catcccgacc gatgacacac ctggagcggt cgaggccgga accatcgact tcctcgaccg
      781 gtatctgtct gggatcgact acatctacgc caaacccgac ggcagcggct ttgaggaact
      841 gtcgggcaag cgcgcggacg cttggcgaca gcgcgtcgag atcatgcgtg agcgttacgt
      901 ctcgggcgta cgcgatcttg acgagcgggc ccacgcagcg cacgacgccg acttcgtgga
      961 cctgacgcca gagcagcagg acggagtgct gcgcgacctc gagctgcccg atcagcagca
     1021 agaaaccgag ctcgctaagg cggaagcgac gctgtacggc gcgccggtcg aacctgcgct
     1081 gcagcagacc agcgcggaag ttgatcttgg tttcgtgcca ctgctggccc tgcatacccg
     1141 gcagggcttc tacgccgacc cgctctacgg cggcaatcgc aatcgagtcg gctgggatgt
     1201 gatcggcttt ccagggccaa cctccctcgc tgaggtgcac agcggccgct acagcaccct
     1261 ggactacttc gcggacgatc gggagcaccc aatcgccaag gaggaggcat cgtgactaac
     1321 ggagcgaagc ccgaccgggc cgacatctgc atcatcgggg ccggggcgtc cggcgccgcc
     1381 gccggcaagg tcctcaccga gcgcgggatg cgcgttgtca tgttggagcg tggcccgtgg
     1441 cgtacccgcg aaaccttcgg cggcgacgaa ctcgccaaca tcaaccgcta caacctgtgg
     1501 ccggacccgc tgctgaaccc ccgcacggtt cgggacgaag tggggggcga gttccgtacc
     1561 gagctgttct gtcccgtgcc gcagatggtc ggcggaggga ccgtgcactg gcaaggctgg
     1621 ctgccgcgct tcaccgaaga ggactttaag ctgcacagcg tcgtcggcga cgtgccggga
     1681 acgacgcttg tcgactggcc catcacttat gacgatcttg aggcgtacta cacccagatc
     1741 gagtgggcgt tcggtgtgtc ggggcgggcg ggcgccaacc gctacgaatc ctggcgtagc
     1801 aaggactacc catgcccccc gctgccccaa tcgaggtatg cacagaagtt ccatcaaggc
     1861 tgcaccgagc tcggctacaa ctcattccct actccacaag ccgcactgtc ccggcctttt
     1921 aacggcaggc cggccacggt gataagcgct ttcgcccagc aacacggaga cccgaccgga
     1981 acccggtcca gcgcgctcaa tgtgttcgtg cccaatgcgg tggcgactgg tcggcttgac
     2041 ctgcggccca acgcctacgt gtcggagatc accgtcgacg agcggggtcg ggcgaagagt
     2101 gcggtgtacg cagacgagga cggcacgacc tacgagcagg aggcaaatgt cttcatcctg
     2161 gcctgcgggg cggttgaaac cgccaggctg ctgctgctct cgaagagttc ccgcttcccg
     2221 aacgggctcg ccaacggcag tgacctggtc ggtcgcaacg tcaccttcca tgagtacagc
     2281 gccgctgtgg gaacattcga ggatccgatc tacgcctggg ccggcggcgg ctacgtcagc
     2341 gccagcagct tcgagttcaa cgggcatgac gacagccgcg gcttcgccgg cggcggccac
     2401 atcgcctgtg cgggcgtcgg catcccgctg ccgatcaact ggtcgctgcc caaccgaccc
     2461 gcttggggag cggaagccaa gcagatcgac cgtgactact tcaaccacag catggccgtg
     2521 gccatggttg tgcacgacct gccgcagcac gacaaccgag tcgagctcga cgactcggtc
     2581 accgacgcct ggggtctgcc ggtcgccaag atcacgatca ttgcgcacga aaacgatctt
     2641 gcgatgggcc gcttcctgat cgatcggaac gccgagatac tcgaggcggc tggagcaaca
     2701 tctgttcaga aggtgtacat cgaccgcatc acgggcaact gctcacacca gcacggcacg
     2761 acgcgcatgg gcaacgaccc ggaaacctcc gtgctggacc gctggtgccg gagccacgag
     2821 gtcgacaacc tcttcgtggt ggacggcagc ccattcccga ccggaaccgg catcaatccc
     2881 accctgacga tcatggcgaa tgcctggcgt gtcgccgaac acattaccaa cggaccccac
     2941 acgacggggc agtagatctg gggcatcggg gcgctccgcg cttacggcaa cggtgtcgcg
     3001 ctggcgaggc ggccggtaat gcagccgcac tttacatcgg aatcggcact gcggagcgcg
     3061 cgtgccgctc gacgccaccc ggacggttgt tgggctgcca tagtgcgccg atccgtgcac
     3121 tatcaacacg atcctcgatc gtatgttctc gccctctcgg agccgacccg gggcatcctt
     3181 gagcgcctcg gacgcggatc ggcgatctca acgggatgaa gagtcgagga ttccgacatg
     3241 cgacgtcccc cgtcgacgcg cgcacgctct gaagagccgc acccacgtgt ccatgtgctt
     3301 cgggcgaaaa cctcaggaga ctccaaggtt gggcttgcaa cagggcgaac acctgccggc
     3361 gaacgccagg caaaatgccg aggacctggt tgacagcggc cggccttggg taacgcgaca
     3421 agcaatttgg cgccacaccc aacccaagag cgcggccgag gaaaggagct gtcgatgagt
     3481 gcttcgtcgg cgatcaccat caaccagacg cagcaggaca tcgccgcacg gttgccgcag
     3541 gctgatccgc cgctgtcgga cgacgacgcg gacgcggaga tcgtgtacgc acctgcccct
     3601 ggggatcggg gcaccgaggt ccgcgtgctg ctacccaaca gcccgggcgg actgggccag
     3661 aaggtcgccg cagtgatggg gctcgacgcg caacgcgagc tcgacgacgc gctgcgccgg
     3721 ttcaagcagc tactggagac cggcgagatt gcgcgctcgg acggcagccc gaccgggact
     3781 gacgccagag cgcaacgcag ccagcaaccc gcccagccaa tgggcgccta caagtaggca
     3841 ggagaaggag aaccatgcga gccaactgtt ggatgggacg caacaccgtc gaggttcgag
     3901 acgttcccga ccctgtcatc ctcaaccccc gcgacgcgat cgtaaagatc acctctactg
     3961 cgatctgcgg atccgatctg ca
//
LOCUS       DASRJA010000381        15200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2835149,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000381 DASRJA010000000
VERSION     DASRJA010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15200
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2835149"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFC01_16890"
     CDS             complement(<1..827)
                     /locus_tag="VFC01_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HZN81345.1"
                     /translation="MTFTATPAVDEAHPCHADIAELTSAYDTAQHISDTCLADGPVGR
                     VGLEVEAHCFDLADPMRRPDWAELTDTIAGVPPLPGGSAITVEPGGAVELSGPPMDDP
                     MAAIGAMTADRAVLRSAFAQAGLGLVLLGADPLRPAKRVNPGDRYLAMERFFIASQTG
                     DAGAAMMTSTASVQVNLDAGPRDGWADRVRLAHALGPTMIAIAANSPLLGGQFTGWQS
                     TRQRVWSQLDSARCGPILGANGDDPASDWARYALKAPVMLMLTPEAVPVTTWVPFAD"
     gene            complement(951..1592)
                     /locus_tag="VFC01_16895"
     CDS             complement(951..1592)
                     /locus_tag="VFC01_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HZN81346.1"
                     /translation="MRTAAAITVGLAFGVCVAAPAHARPSDPGVVNYAVMGKGSVSNI
                     VGAPMRFAWTYTDPFQSYYVDEPLCNNWADIGLPEVYADPDLASFNGAVTQTGDNDQN
                     HYAKQAVGVFATTDAADRAFHRVVDRTVGCSGQTTAMHLDNFTTQVWTFTGGPASATD
                     ADWVKQEAGTDRRCFTTTRKRENVLLQAKVCQSGNAGPAVNVLAGAMQNTLGQ"
     gene            complement(1597..2025)
                     /locus_tag="VFC01_16900"
     CDS             complement(1597..2025)
                     /locus_tag="VFC01_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HZN81347.1"
                     /translation="MSIEVVFTAESTATGGGRDGHVKSSDGRIDLDTRPPKEAGGNGE
                     GTNPEQLFSAGYAACFLGALRLVARNNDIKLDDATGITAQVGFGKDPAGGFGINAQLI
                     GYLPGLEQSAADDLMEKAHQVCPYSKATRGNIDVTLSAKV"
     gene            complement(2107..2526)
                     /locus_tag="VFC01_16905"
     CDS             complement(2107..2526)
                     /locus_tag="VFC01_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81348.1"
                     /translation="MSETTSSPEPATGPVATTTPPPDAPPPAYAEPQRHSRLTAVAAW
                     VGIVAGVVFIVAVIFFSGFFLGAHSGGHRGGHHGGGDREFGIIHKGPPPMFPMGPRGE
                     FERPAPPFGPGGPSFQGPQGPSPQQPGGPTTTAPRPS"
     gene            complement(2924..4048)
                     /locus_tag="VFC01_16910"
     CDS             complement(2924..4048)
                     /locus_tag="VFC01_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HZN81349.1"
                     /translation="MRVQACWNTVSAVLAILVLVAPAAHADTAAPIPEPILPPLAPGQ
                     VIRIAPAAGTGTPTRDYNIGATDLCEFMEFPDGILQVCGDSFAGQGVGFGPWHSPIAL
                     HVDTDSIGDPAGVRYDRVMGNDQLLLADPTPTGMSQLPAGVVQINRDNYLMISTVKDL
                     VPQTSRLVKTNPGQGNWPTIPNTQRPATYEGGRQSQISGYYDPIPKPDSPSGWVYIVA
                     NNFDRSGPVVLYRAKKETFTDRGTWQGWSAQGGWGKPPTPLWSDRIGEMSIRQIDGRT
                     VLSYFNASTGNMEARVADDPTGLGAAPVTTVVVAGPWPDPAEDLGPPENNQLAQPYGG
                     YLSPGSTLDELRVFISQWNTGPGDNAPYRVIQFAVNPLKP"
     gene            complement(4080..5117)
                     /locus_tag="VFC01_16915"
     CDS             complement(4080..5117)
                     /locus_tag="VFC01_16915"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZN81350.1"
                     /translation="MMVNIGVVGATGQVGQVMRALLEERDFPATSVRFFASARSQGKK
                     LPFRGQEIEVEDASTADPSGLDIALFSAGATMSRVQAPRFAAAGVTVIDNSSAWRKDP
                     DVPLVVSEVNFTRDAHNRPKGIIANPNCTTMAAMPVLKPLHDEAGLVRMIASTYQAVS
                     GSGIAGVDELFGQANAVVGNSKELVADGSALDFPAPNKYVAPIAFNVVPLAGSYVEDD
                     SGETDEDQKLRNESRKILGIPDLLVSGTCVRVPVYTGHSLSINAEFERPLSVEHARHL
                     LAEAPGVKVVDVPTPLAAAGVDESLVGRIRQDPGVPDGRGLALFVSGDNLRKGAALNT
                     IQIAELLAAEL"
     gene            complement(5114..6379)
                     /locus_tag="VFC01_16920"
     CDS             complement(5114..6379)
                     /locus_tag="VFC01_16920"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZN81351.1"
                     /translation="MALVVQKYGGSSVSDAERIRRVAERIVETKKQGNDVVVVVSAMG
                     DTTDDLLDLAKQVCPAPPAREMDMLLTAGERISNALVAMAIDSLGAQARSFTGSQAGV
                     ITTSIHGNAKIIDVTPSRLQAALDEGLIVLVAGFQGVSQDSKDVTTLGRGGSDTTAVA
                     LAAALKADVCEIYTDVDGIFTADPRIVPNARRMDTVSFEEMLELAACGAKVLMLRCVE
                     YARRYNVPVHVRSSYSDKPGTIVTGSIEDIPMEDAIITGVAHDRSEAKVTIVGIPDVP
                     GYAAKVFRAVADADVNIDMVLQNISKIEDGKTDITFTCSRESGPGAVEKLTSLQEEIG
                     FSKVLYDDHIGKVSLVGAGMRSHPGVTAKFCEALAGVGVNIDLISTSEIRISVLIKDT
                     ELDKAVVALHEAFELGGDEAAVVYAGTGR"
     gene            complement(6529..6981)
                     /locus_tag="VFC01_16925"
     CDS             complement(6529..6981)
                     /locus_tag="VFC01_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81352.1"
                     /translation="MKLDRRWWVAIGVAVVAAVALVLQYTVFDRPSEECRPVKELLDF
                     NRSQGEQIASKTGDEQGIPTAAEETAYQAWADGLAERAQEVNSPDLATTATTVAGLAN
                     EFVGKLSIVRAQAESRAPGAPAPPAAYEMMALNARISENLDELAKACS"
     gene            7225..8478
                     /locus_tag="VFC01_16930"
     CDS             7225..8478
                     /locus_tag="VFC01_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZN81353.1"
                     /translation="MDTGTTAFMLCCIIGLTVMIPGLALFYGGMVSVKSSTNMMMMTF
                     GAVAIVGVLWVLFGFSMAFGTSYGGFVGSFTEFAGMKNLTEPMTTINGLPVSLFALFQ
                     ALFAAITVALISGAVADRMKFGAWMTFATAWAILVYFPVAHWVFAFDGVVTPDATGGW
                     IANKLKAIDFAGGTAVHINSGAAALAVAIVLGKSLGFGKFRKPHNVPLTLLGAGLLWA
                     GWYAFNGGSALAAGTSAAIVMVTTFTATCAATLAWLTVEKFKDGHVTGVGAASGAVAG
                     LVAITPACGAVTPIGSIFVGLIAGAICPLAVGLKERFGYDDSLDVVGVHLVGGVIGTL
                     LIGLFASAGMPNKTDGLFYGGGLDQLWKQAIAAGAVMAYSFVVAYIIAFVIKKTMGIR
                     ISADEEEKGIDAKFHRDAAYELQPA"
     gene            8551..9855
                     /gene="glnT"
                     /locus_tag="VFC01_16935"
     CDS             8551..9855
                     /gene="glnT"
                     /locus_tag="VFC01_16935"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HZN81354.1"
                     /translation="MSSDLATLAENTNTKFILALFVDLRGKPCAKLVPVEAVDLLATE
                     GVGFAGYAVGAMGQEPKDPDLMAIPDATSFTPIPFIKDGLAIVHCDPHVNGKPWPYAP
                     RVILKSLIQQTADAGYEPWVGAEVEYFLLHRNSDGSLSVADAADTAAQPCYDARGVTR
                     MYDHLTAISAAMNTLGWSNYANDHEDGNGQFEQNFQFADALTTADRVVTLRYLLSMIA
                     AERGMVATFMPKPFAEKTGSGLHLHLSLTSGGTPVFPADDDSRGLGLSETAYAFIGGI
                     LEHAGALQAVVAPTVNSYKRTGATTTASGASWAPRTPTYGGNDRTHYIRVPDSARIEL
                     RGSDGSANPYLAIAAALGAGIDGVKRSADPGDIGAQGHSPLPPTLLHAVDEFEGDPVV
                     AGVLDAAGDGVAAYFANVKREEFFAYHSAVTPWEIDHYLTAF"
     gene            9883..10755
                     /locus_tag="VFC01_16940"
     CDS             9883..10755
                     /locus_tag="VFC01_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HZN81355.1"
                     /translation="MCGIVGLHLRTPELYPRLGELLTGMLCEMGNRGSDSAGVAVYGD
                     PIWSPPGRGCVSVLEVRPAADVLGRDVNVTQVGDTYLLTADVTSEVLLDAVRAAYPQA
                     LIAGFGSDMAVFKGVGHPRALTESWGLAGAQGWQGVGHTRMATESAVTPAGCHPYAVG
                     PEQCMVHNGSFANHATIRRELRAAGVQFDSENDTEVGARFIAKQLADGRDVEAALKEL
                     CAVFDGFYTLLVSNRDSFAVVRDAIACKPAVIAETDEWVAMGSEYRALAGLPGVENAK
                     IWEPEPEVIYAWKK"
     gene            10743..11441
                     /locus_tag="VFC01_16945"
     CDS             10743..11441
                     /locus_tag="VFC01_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein glxC"
                     /protein_id="HZN81356.1"
                     /translation="MEKVTATMSLYDLHTTPLREVNSALHQPHLSGAFVIAHPDGAHN
                     VAVGVDAPVKVTVEGHVGYYAAGMNQHAEITINGNAGTGVAENMMSGTVWVRGNASQS
                     AGATAHGGLLVIEGNAAARCGISMKGVDIVVGGDVGHMSAFMAQAGRLVIRGDAGEAL
                     GDSIYEARIYVRGDVASLGADCVAKEMRPEHQKELEYLLKTAGFEADDTAGYTRYGSA
                     RNLYHFHVDNASSY"
     gene            11443..12765
                     /locus_tag="VFC01_16950"
     CDS             11443..12765
                     /locus_tag="VFC01_16950"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HZN81357.1"
                     /translation="MNTDQRALAGLRESATFDRPTIAAIQRAAETGIYDIRGWGAKRP
                     LPHFDDLLFLGASMSRYPLEGYRERCGTDVVLGDRFAKHPLHLDIPVTIAGMSFGALS
                     GPAKEALGRGASEAGTSTTTGDGGMTPEERGQSKYLVYQYLPSRYGMNPADLRKADAI
                     EVVLGQGAKPGGGGMLLGQKISERVAGMRTLPEGIDQRSACRHPDWTGPDDLTIKINE
                     LREITDFQKPIYVKVGATRTYYDVKLAVHAGADVVVVDGMQGGTAATQEVFIEHVGIP
                     TLAAVPQAVQALQELGVHRKVQLIVSGGIRTGADVAKALALGADAVAIGTAALIALGD
                     NHPRYAAEYEKLGSAAGFFDDFQDGTDPAGISTQDPELAARFDPVEGGRRLANYLRVL
                     TMEAQTIARACGKAHVCHLEPEDLVAVTIEAAAMARVPLAGTNWVPGQ"
     gene            12762..13946
                     /locus_tag="VFC01_16955"
     CDS             12762..13946
                     /locus_tag="VFC01_16955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN81358.1"
                     /translation="MTTADVVIVGGGIEGSAAAWALSQRGVTDVVVLERNTVASGMTG
                     KSSGIVRCHYGVTSLAAMATVGLEVFEQAEDIFGTDIGFRQTGYVVGVGEPNVESLRK
                     SLAAQRAVGVQTEEIDKSEVARMWPFADLTPFEAFGWEARGGYGDAYMTAQAFAASAR
                     TAGVRIRQGATVTELLLQGDRVTGVRLADGDQISAGTVVVATGVWTKPFLAPYGIEVP
                     MRVVREQIVTIAPGLDIGSVPVFSDLVSLQYVRPEVGGDILFGNSDLSDVEQTDPDNY
                     LNRATDTFVDLTVDKVGTRFPGFTDAAITGSYAGCYDVTPDWNPVISRTDLGGLFVAA
                     GFSGHGFKIAPAVGRLVADLVVDGHSTDPRIPHSDFRLSRFDEGDLLRSPYPYVGAGQ
                     MR"
     gene            13962..14609
                     /locus_tag="VFC01_16960"
     CDS             13962..14609
                     /locus_tag="VFC01_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZN81359.1"
                     /translation="MSDVPLLRNKSGTARDRDPHEPVDELEIEAAIGRNVRQLRQQQG
                     LTVAEMAARVGISKAMMSKIENAQTSCSLSTLALLAKGFDVPVTSLFRGADVERPAAF
                     VKAGTGARIVRNGTKEGHEYQLLGSLRGEHKRLECLHVTLSEKSQTYPLFQHPGTEFI
                     YMLEGVMDYSHSRSVYRLHPGDSLQIDGEGAHGPVDLVELPIRFLSVIAFPDSQV"
     gene            complement(14600..>15200)
                     /locus_tag="VFC01_16965"
     CDS             complement(14600..>15200)
                     /locus_tag="VFC01_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81360.1"
                     /translation="DYLVPLNLSVLPGAQPLRKPDQPFVRVSLIAEEDCDFAQALVRA
                     IAPRLESLQLPVRDAVATYPSAVAERDPCEVMSVLGADIDGWDITRSRPYECSFGIFR
                     SGFRDIVPIQVSLKPQVYDEATETRARRETDGVEIFVDKTYCSAVAFVGAPMLRKLVG
                     GDYVDAGDVVIRPAVVVDSGGEHCDVVTDVAMAAAKLYT"
BASE COUNT         2544 a         5044 c         5004 g         2608 t
ORIGIN      
        1 cagtcggcga acggcaccca ggtggtcaca ggcaccgcct caggcgtgag catgagcatc
       61 acgggcgcct tcagcgcgta ccgggcccag tcgctggccg ggtcgtcacc gttggcaccg
      121 aggatcgggc cgcagcgcgc cgagtccagc tggctccaca cccgctggcg cgtcgattgc
      181 caaccggtga actgtccgcc cagcaatggc gagttggcgg cgatcgcgat catcgtcggg
      241 cccaacgcat gcgccagccg cacccggtcc gcccagccgt cccgcgggcc tgcgtcgagg
      301 ttgacctgta ccgacgccgt cgacgtcatc atcgccgcgc ccgcgtcgcc agtctggctc
      361 gcgatgaaga atcgctccat cgcgaggtat cgatcgcctg ggttcacccg cttggccggg
      421 cgcagcgggt cggcgcctag cagcaccaga ccgaggccgg cttgcgcgaa cgcggatcgc
      481 aacacggcgc ggtcggccgt catcgcgccg atagccgcca ttggatcgtc catcggcggg
      541 ccggacaatt cgaccgcgcc gccgggctcg accgtgatcg cgctgccgcc gggcagtggc
      601 ggcacgccgg cgatggtgtc ggtcagctcg gcccagtcag ggcggcgcat cggatcggcg
      661 aggtcgaagc aatgcgcctc gacttcgagg cccacccgac ccaccggccc gtcggccaag
      721 caggtgtcgg agatgtgctg tgcggtgtca tatgcgctgg tcagctcggc tatatcggcg
      781 tgacatgggt gcgcttcgtc gaccgcgggc gtggcggtga aggtcatatc ttccggcccc
      841 tttcgggtag ggaacgatcg cggccttcac tccatcttcc agagcccgcc gacaaattcc
      901 cgcgattttt ggatgacgcg ttcgtgacga gtaccccaaa aggttgccga ttactgcccc
      961 agtgtgttct gcatcgctcc ggccagcaca ttcaccgcgg gcccggcgtt gccagattgg
     1021 cagactttcg cctgcagcag aacgttctcc cgcttgcggg tggtggtgaa gcagcgccgg
     1081 tcggtgccgg cctcctgctt cacccagtcg gcgtcggtgg cgctggccgg cccgccggtg
     1141 aaggtccaca cctgggtggt gaagttgtcc agatgcatcg ccgtcgtctg cccgctgcat
     1201 cccactgtcc ggtcgacgac ccggtggaaa gcgcggtccg ccgcgtcggt ggtggcgaac
     1261 acaccgaccg cctgcttggc gtagtgattc tggtcgttgt cgccggtctg ggtgaccgcg
     1321 ccgttgaacg acgcgaggtc ggggtcggca tagacctcgg gcagcccgat gtcggcccag
     1381 ttgttgcaga gcggttcgtc gacgtaatac gactggaacg gatccgtgta ggtccacgcg
     1441 aaccgcattg gcgcgccgac gatgttgctc accgaaccct tgcccatcac ggcgtaattg
     1501 accacgccgg gatccgacgg acgagcatgg gccggcgccg ccacgcacac accaaacgcc
     1561 agaccgacgg ttattgctgc ggcggtccgc accggcttag accttcgccg acagcgtgac
     1621 gtcgatgttg ccgcgggtgg ccttggagta cgggcacacc tgatgcgcct tctccatcaa
     1681 gtcgtcggcg gcgctctgct cgaggccggg caggtagcca atcagttggg cgttgatgcc
     1741 gaacccgccc gcggggtcct tgccgaagcc gacctgggcg gtgatcccgg tggcgtcgtc
     1801 gagcttgatg tcattgttgc gcgcaacaag acgaagggca ccgaggaagc atgccgcgta
     1861 ccccgcggag aacagctgct cggggttggt gccctcgccg tttccacccg cttccttcgg
     1921 cggccgggtg tccagatcga ttcggccgtc ggacgacttc acatggccgt cgcgaccgcc
     1981 cccggttgcg gttgactctg cggtgaaaac gacttcgatg ctcatgggct tgatcatgcc
     2041 agccgggtcg cagacggcaa gacgccccgg tcgagcttgg accggggcgt cttagcggtc
     2101 aagcgattac gacggacgcg gagcggtcgt ggtgggtccg cccggctgtt gcggcgatgg
     2161 cccctgaggc ccctggaagc tcgggccgcc cggtccgaac ggcggggcgg ggcgttcgaa
     2221 ttcgccgcgc ggacccatcg ggaacatcgg cggcggtcct ttgtggatga tcccgaattc
     2281 gcgatcgcca ccgccatgat gcccaccgcg gtgcccgccg gagtgcgcgc cgaggaagaa
     2341 gccggagaag aagatgacgg cgacgatgaa gacgacgccc gcgacgatgc cgacccaagc
     2401 ggcgaccgcg gtcagccggc tgtggcgttg cggctcggca tacgccggtg gtggcgcgtc
     2461 gggcggcggt gtggtggtgg ccaccgggcc ggttgcaggc tcaggggacg acgttgtttc
     2521 gctcatgcac ccgagcatgc aagcggtgcc gatgtgtccg tcttgaattg gttatgaatc
     2581 agctgtgaat ggcggcggcc gttgaagtgc agacggcaca tagctcctga cccagcgtcg
     2641 gatatccgca tgccgcacag atcgcaaccg agttcccact agaagaggtc gtgctggcca
     2701 ccgcggttcc tttcgcccgg tgcggagcgt gtaccccctt cagtgaacat tcatgccacc
     2761 ctcccgcaaa ggattcaccg aggcgtggcg cgggtactga tgcagacatg aacagcgacg
     2821 cgatcatggc gctgtcgggg tttatatgcc tggctgccgc cgcgctgacc ggggcacttt
     2881 gggacacccc gttggtgacg acaccgttcc ggtggcttgt gagctagggc ttcagcggat
     2941 tcactgcaaa ctgaatgacc cggtacgggg cgttgtcgcc cgggccggta ttccactggc
     3001 tgatgaacac ccgtagttcg tctagcgtcg agcccggcga gaggtagccg ccatacggct
     3061 gggcgagctg attgttctcc ggtggcccca ggtcctccgc gggatcgggc cacgggcccg
     3121 cgacgacgac cgtcgtgacc ggtgcagcgc cgagaccggt cggatcgtcg gcgacgcggg
     3181 cctccatgtt gccggtactg gcgttgaagt aagacaacac ggtcctgccg tcgatctgcc
     3241 ggatgctcat ttcgccgatt cgatccgacc acaacggcgt tggcggcttg ccccagccgc
     3301 cctgagcaga ccacccttgc caggttccgc ggtcggtgaa cgtctccttc ttggcccggt
     3361 acagcacgac gggaccgctg cggtcgaagt tgttggcgac gatgtacacc caaccgctcg
     3421 gcgaatccgg cttcgggatc gggtcgtagt agccgctgat ctgtgactgc ctgccgccct
     3481 cgtaggtcgc gggccgctgc gtgttcggaa tcgtcggcca attgccttgt cctggattgg
     3541 ttttcaccag ccgagacgtc tgcggcacca agtccttgac cgtcgagatc atcagatagt
     3601 tgtcccggtt gatctgcacc accccggcgg gcaactgcga catccccgtt ggtgtcggat
     3661 cggccaacaa gagctgatcg ttgcccatca cccggtcgta gcgcacaccc gcgggatcgc
     3721 cgatcgagtc ggtgtcgacg tgcaacgcga tcggcgaatg ccagggaccg aatccgacgc
     3781 cctgtccggc aaagctgtct ccgcacacct gcaggatgcc gtcggggaac tccatgaact
     3841 cgcacagatc ggtcgcaccg atgttgtaat cccttgtcgg cgtgccggtc ccggccgccg
     3901 gtgcgatgcg gatcacctgg ccgggcgcca gcggaggcag gatcggctca gggatgggcg
     3961 ctgccgtatc ggcatgcgcc gccggggcaa cgaggaccaa gatggccagt accgccgaaa
     4021 ctgtattcca gcaggcttgt actcgcactt tctctgctgg aataccgttt cggcacaaat
     4081 tacagctcgg cggccagcag ctcagcgatc tgaattgtgt tcagcgccgc cccttttcgc
     4141 aggttgtcgc cggacacgaa caatgccagt ccacggccgt cgggtacacc tggatcttgg
     4201 cggatacggc cgacgagtga ttcgtcgacg cccgcggcag ccaacggcgt tggcacgtcg
     4261 acaaccttca ctccgggcgc ctcggccagt agatggcggg cgtgttccac cgacagcggc
     4321 cgctcgaact cggcgttgat cgacagcgag tgcccggtgt acaccggcac ccgcacacaa
     4381 gttccgctca ccagcaggtc gggaatcccg aggatcttgc ggctctcgtt gcgcagcttc
     4441 tgatcttcgt cggtctcacc ggaatcgtcc tcgacatacg aaccggccag cggcacgacg
     4501 ttgaacgcga tcggcgcgac gtacttgttg ggcgccggaa agtccagcgc cgaaccatcg
     4561 gccaccagct ccttgctgtt gccgacgacg gcgttggcct gcccgaacag ctcatcgaca
     4621 cccgcgattc cgctgcccga caccgcctga tacgtcgacg cgatcatccg caccagaccc
     4681 gcctcgtcgt gcagcggctt gagcacgggc atcgcggcca tcgtggtgca attcgggttg
     4741 gcgataatgc ctttcggccg gttgtgtgcg tcgcgggtga agttgacctc gctgaccacc
     4801 agcggcacgt cggggtcttt gcgccacgcc gacgaattgt cgatgactgt cacccctgcg
     4861 gcggcgaagc ggggcgcctg cacccgcgac atcgtcgcac cggccgagaa cagcgcgatg
     4921 tccaagccgg acgggtctgc tgtcgatgcg tcttcgacct cgatctcctg gccgcggaac
     4981 ggcagcttct tgccctgcga ccgcgcggag gcgaagaacc gcacgctggt ggcgggaaaa
     5041 tccctctcct ccaacagggc tcgcatcacc tggccgacct gcccggtcgc accaactacc
     5101 ccgatgttga ccatcagcgt cccgttcccg cgtagacgac ggccgcctcg tcgccgccaa
     5161 gctcgaatgc ctcgtgcagg gcgacgaccg ccttgtccag ttcagtgtcc ttgatcagca
     5221 ccgagattcg gatctccgag gtggagatca ggtcgatgtt gacgccgact ccggcgagtg
     5281 cctcgcagaa tttcgcggtg acgccgggat ggctgcgcat gcccgcgccg accaacgaca
     5341 ccttgccgat gtgatcgtcg tagagcacct tggaaaagcc gatctcttct tggagcgatg
     5401 tcagcttttc cacggcgccc ggcccgctct cccgtgagca ggtgaacgtg atgtcggtct
     5461 tgccgtcctc gatcttggag atgttctgca gcaccatgtc gatgttcacg tcggcgtcgg
     5521 cgaccgcgcg gaacaccttg gcggcgtagc cgggaacgtc ggggattccg acgatcgtca
     5581 ccttcgcctc gctgcgatcg tgggcgactc cggtgatgat ggcgtcttcc atggggatgt
     5641 cctctatcga tccggtcaca atcgtgccgg gcttatctga atacgacgac cggacgtgaa
     5701 ccggaacgtt gtagcggcgg gcatattcca cgcagcgcag catcagcacc ttggcgccgc
     5761 acgcggcgag ctcgagcatc tcctcgaacg acacggtgtc catccgccgc gcgttgggca
     5821 cgatgcgcgg gtcggcggtg aagatgccgt cgacgtcggt gtagatctcg cagacgtcgg
     5881 ccttcagggc tgcggccagt gcgacggcgg tggtgtccga accaccgcgg ccgagcgtgg
     5941 tgacgtcctt gctgtcctgg ctgacgccct ggaatccggc gaccagcacg atcagtccct
     6001 cgtccagggc tgcctgcaac cggctgggtg tgacgtcgat gatcttggcg ttgccgtgga
     6061 tgctggtggt gatcacgccg gcctgcgacc cggtgaacga gcgggcctgc gcgcccagcg
     6121 agtcgatggc catcgcgacc agcgcattgg agatccgctc accagcggtc aggagcatgt
     6181 ccatctcgcg cgccgggggc gccggacaca cctgcttggc cagatcgagc aggtcgtcgg
     6241 tggtgtcgcc catcgccgag accacgacga ccacgtcgtt cccctgcttt ttggtttcca
     6301 cgatgcgttc ggcgacgcgc cggatccgct cggcatcaga aaccgaggat ccgccgtatt
     6361 tctgtacgac gagcgccact gcagaacctt tcttgggcgg ggttcatcta agaataaagg
     6421 gtctgcgcac tccatttact ccgcccgtag cgtcaagatg tggtcaagag gctcgcgcgc
     6481 acccgcgtac cgatccaccc ccacatcgcc gcacgcggtt gaccatcgtc aagaacaggc
     6541 cttcgccaac tcgtcgaggt tctcggagat tcgggcgttc agcgccatca tctcgtaggc
     6601 cgccggcggc gccggagcgc cgggagctcg tgactctgct tgggcgcgca cgatcgaaag
     6661 cttgccgacg aactcgttgg cgagaccggc gaccgtggta gccgtggtgg ccaggtcagg
     6721 cgagttcacc tcttgggcac gttcggccag cccgtcggcc cacgcctggt atgcggtttc
     6781 ttcggccgcg gtcgggatgc cctgctcgtc gccggttttc gaggcgattt gctcgccctg
     6841 ggagcggttg aagtccaaaa gctccttgac cggacggcat tcctcggacg gacggtcaaa
     6901 cacggtgtac tgcaacacca gagccacggc tgccaccact gcgacgccaa tggcgaccca
     6961 ccatcgccga tcgagcttca ccgcacgaac tgtacgggcg gccgactgtc gtttgcggag
     7021 gtggccttcg ccgacggctc ggcccagctc ctcgaaactg cgcggagatc gctaaaaagc
     7081 gaccgatcgc gatctggccg cagtctcgcg cgtcgctcaa gctttaaccc ggccgaaaca
     7141 ccgcgagcct gtccacgtaa cagaaacatc ggttactggc agtgaacggc tctcaccgga
     7201 gccgcaccac tccaggagat gtaaatggat acaggaacca cagcattcat gctgtgttgc
     7261 atcatcggcc tcacggtgat gattcccggg ctcgcgctct tctacggcgg catggtgtcc
     7321 gtcaagagct cgaccaacat gatgatgatg accttcggcg ccgtcgccat cgtcggcgtg
     7381 ctgtgggtgt tgttcggctt ctcgatggcg ttcggcacgt cctacggcgg cttcgtcggt
     7441 agcttcaccg aattcgccgg catgaagaac ctgaccgaac cgatgaccac catcaacggc
     7501 ctgccggtga gcctattcgc cctattccaa gcacttttcg cggcgatcac cgtggcgctg
     7561 atatccggcg cggtcgccga ccggatgaag ttcggcgcct ggatgacgtt cgcgaccgcg
     7621 tgggcgatcc tcgtgtactt ccccgtggcg cactgggtgt tcgcgttcga cggcgtggtg
     7681 acgccggacg ccaccggcgg ctggatcgcc aacaagctca aggcaattga cttcgccggt
     7741 ggcaccgcgg tgcacatcaa ctccggcgcc gcggccctgg ccgtcgcgat cgtgctgggc
     7801 aagtcgctgg gcttcggtaa gttccgcaag ccgcacaatg tgccgctgac gctgctcggc
     7861 gcgggcctgc tgtgggccgg ctggtacgcg ttcaacggcg gttcggcgct ggccgcgggt
     7921 acctcggccg ccatcgtaat ggtcacgacg ttcacagcaa cctgcgcggc gacgctggcc
     7981 tggctcacgg tcgagaagtt caaggacggg cacgtcaccg gcgtcggcgc agcgtcaggc
     8041 gcggtggccg gactggtcgc catcactccc gcctgcggtg cggtcacccc gatcggctcg
     8101 atcttcgtcg gcttgatcgc gggcgcaatc tgcccgctgg cggttgggct gaaggagcgg
     8161 ttcggctacg acgactcgct tgacgtggtc ggcgtgcacc tggtcggcgg cgtgatcggc
     8221 accctgctga tcggcctctt cgccagcgcg ggcatgccga acaagaccga cggcctgttc
     8281 tacggcggcg ggctcgatca gctgtggaag caggcgatcg ccgcgggcgc ggtgatggcc
     8341 tactccttcg tggtcgctta catcatcgcg ttcgtcatca agaagacgat gggcattcgc
     8401 atctcggccg acgaagaaga aaagggcatc gacgccaagt tccaccggga cgcggcgtac
     8461 gaactgcagc ccgcctgacg gaccccggcc ggcgcggaac actcgcccgc gccggccggc
     8521 ccttcacctc aagagaaggc ttgtacagac atgtcctcag accttgccac cctcgccgag
     8581 aacaccaata cgaagttcat tcttgcgctg ttcgtcgacc tgcgaggcaa gccctgcgcg
     8641 aagctggtgc ccgtcgaagc ggtagacctg ctcgcgaccg agggcgtcgg cttcgccgga
     8701 tacgccgtcg gcgccatggg ccaggagccc aaagaccctg acctgatggc gattccggac
     8761 gcgacgtcgt tcacgccgat cccgttcatc aaggacggtc tggcgatcgt ccactgcgac
     8821 ccgcatgtca acggcaagcc gtggccctat gccccgcgcg tgatcctcaa gtcgctcatt
     8881 cagcagacgg ccgacgcggg ctacgagccg tgggtcgggg ccgaggtcga atacttcctg
     8941 ctgcaccgca attccgacgg cagcctgtcg gtcgcggatg ccgccgacac cgccgcgcag
     9001 ccctgttacg acgcccgcgg cgtcacccgg atgtacgacc acctgaccgc gatctcggcg
     9061 gcgatgaaca cgctcggctg gtccaactac gccaacgacc acgaggacgg taacggccag
     9121 ttcgaacaga acttccaatt cgccgacgca ctgaccaccg ctgaccgagt cgtgacgctg
     9181 cggtatctgc tgtcgatgat cgcggccgaa cgcggcatgg tggccacttt catgcccaag
     9241 ccgttcgccg agaagactgg cagcggtttg catctgcact tgtcgctgac cagcggcggc
     9301 acgcccgtct ttccggccga cgacgactcc cgcggacttg gcctgtcgga gactgcgtat
     9361 gcgttcatcg gcgggatcct cgaacacgcc ggcgcgctgc aggcggtcgt ggcgccaaca
     9421 gtcaactcct acaagcgaac cggtgcaacg acgacggcat cgggtgcgtc ctgggcgccg
     9481 aggacgccga cgtacggcgg caacgaccgc acgcactaca tccgcgtacc cgactccgcg
     9541 cgcatcgagc tacgcggcag cgacggttcc gccaacccgt acctggcgat cgccgcggcg
     9601 ctcggggcag gtatcgatgg agtcaagcgc agtgccgatc ccggagacat tggcgcccag
     9661 gggcattcac ctcttccacc gacgctgctg catgccgtcg atgagttcga gggcgacccg
     9721 gtggtcgccg gtgtgctcga cgccgcgggc gacggtgtgg cggcctactt cgccaacgtc
     9781 aagcgcgagg agttcttcgc ttaccacagt gccgtgacgc cgtgggagat cgatcactac
     9841 ctgaccgcat tctgaatcgt cgcaagtcaa aggagacaag tcatgtgcgg gatcgtcggg
     9901 ttgcacctgc gcaccccgga gctgtatccc cggctcggag agctgctcac cggaatgctg
     9961 tgcgagatgg gtaaccgcgg cagcgactcg gccggggtcg ccgtctacgg cgacccaatc
    10021 tggtcaccgc caggacgggg atgcgtctcg gtacttgaag tgcgaccggc cgccgacgtt
    10081 ctcggccgcg acgtcaacgt gacgcaggtt ggcgacacgt atctccttac cgcagacgtg
    10141 acttcagagg tactgctgga cgcggtcagg gcggcgtatc cacaggcgct gatcgccggc
    10201 ttcggctcgg acatggccgt cttcaagggc gtcggacatc cgcgcgcatt gaccgagagc
    10261 tgggggctgg ccggggcgca gggctggcag ggcgtcgggc acaccaggat ggcgaccgag
    10321 tcggcggtga cgcccgcggg ctgtcatccc tacgcggtcg gccccgagca gtgcatggtg
    10381 cacaacggct cattcgccaa ccacgcgacg atccgtcgcg aactacgggc cgccggtgtg
    10441 caattcgaca gcgagaacga caccgaggtc ggtgcgcgct tcatcgccaa gcaattggcc
    10501 gatggtcgcg atgtggaggc agcgctcaag gagctgtgcg ctgtctttga tggtttctac
    10561 acgctgctgg tgtccaaccg cgactcgttc gcggtggtgc gcgacgcaat cgcctgcaag
    10621 ccggccgtca tcgccgaaac cgacgaatgg gtggcgatgg gcagcgaata ccgcgcgctg
    10681 gcaggccttc ccggcgtcga gaacgccaag atctgggagc cggagccgga ggtgatctac
    10741 gcatggaaaa agtgacagcc acgatgtcct tatatgacct ccacaccacg ccgctgcgag
    10801 aggtcaactc ggcgttgcac cagccgcacc tgtccggtgc gtttgtgatc gcgcatcccg
    10861 acggcgcgca caatgtcgcc gtcggtgttg acgcaccggt gaaggtgact gtcgagggcc
    10921 acgtcggcta ctacgccgcg ggcatgaacc agcatgcgga gatcaccatc aacggtaatg
    10981 cgggcaccgg cgtggccgag aacatgatga gcggcacggt ctgggtcagg ggcaacgcgt
    11041 cgcaatcggc tggcgcgacc gcgcatggtg gcctgctggt gatcgagggc aatgccgcag
    11101 cgcggtgcgg gatttcgatg aagggcgtcg acatcgtggt aggcggcgat gtcggtcaca
    11161 tgagtgcgtt catggcgcag gccgggcggc tggtgatccg cggcgacgct ggcgaggcgc
    11221 tcggcgactc gatctacgag gcgcgcatct acgtccgcgg cgacgtcgca tcccttggcg
    11281 ctgactgcgt ggccaaggag atgcgtcccg aacatcaaaa ggaactcgaa tacctgctca
    11341 agacagccgg tttcgaggcc gacgatacgg ccggctacac ccgctacggt tcggcgcgca
    11401 acctctatca cttccacgtc gacaacgcat cgagctactg agatgaacac tgatcagcgg
    11461 gcgctagcgg gattgcgcga atccgccacc ttcgaccggc cgactatcgc ggccatccag
    11521 cgcgccgcag agaccggcat ctacgacatc cgcggctggg gcgccaagcg gccgctgcca
    11581 catttcgatg acctgctatt cctcggtgcg tcgatgtcgc gttacccgct ggaggggtac
    11641 cgcgagcggt gcggtaccga cgtcgtcctc ggcgaccggt tcgcgaaaca tccactgcac
    11701 ctcgatattc cggtgacgat cgccggcatg tcgttcggtg cgctgtccgg gcccgccaag
    11761 gaagccttgg ggcgcggtgc cagcgaggcg ggcacgtcga ccactaccgg cgacggtggg
    11821 atgactcccg aggaacgcgg acagagcaaa tacctggtct accaatacct tccgtcgcga
    11881 tacgggatga accccgccga cctgcgcaag gccgatgcga tcgaggtggt cctcggtcag
    11941 ggcgccaaac ccggcggcgg cggaatgctg ctgggccaga agatctccga acgcgtggct
    12001 gggatgcgca ccctgcccga gggcatcgac cagcgctcgg cgtgcaggca ccccgactgg
    12061 accggtcccg acgacctgac catcaagatc aacgaactgc gcgagatcac cgacttccag
    12121 aagccgatct acgtcaaggt cggcgccacc cgcacgtact acgacgtcaa actcgccgtg
    12181 cacgcaggcg ccgacgtggt ggtcgtcgac ggcatgcagg gcggcaccgc cgccacccag
    12241 gaggtgttca tcgagcacgt cggcatcccg acgctggccg ccgtgccgca ggcggtgcag
    12301 gcgctgcaag agctcggcgt gcatcgcaag gtgcagctga tcgtctccgg tggcatccgc
    12361 accggcgccg atgtcgccaa ggcgctggcc ctcggcgccg acgccgtggc gatcggcacc
    12421 gccgcgctga tcgcgttggg cgacaaccat cctcgctacg ccgccgaata cgagaagctc
    12481 ggcagcgccg caggcttctt cgacgacttc caggacggca ccgacccggc ggggatcagc
    12541 acccaggacc ccgaactcgc cgcgcggttc gaccccgtcg agggcggccg gcggctggcc
    12601 aactacctgc gggtgctgac catggaggcg cagaccatcg cccgcgcgtg cggcaaggcg
    12661 cacgtgtgcc acctcgagcc cgaagacctc gtcgccgtca ccatcgaggc ggccgccatg
    12721 gcgcgggtgc cgctcgccgg tacgaactgg gtccctggtc aatgaccacc gcggacgtcg
    12781 tcatcgtcgg cggcggcatc gagggcagcg ccgcagcgtg ggcgctgagc cagcggggtg
    12841 tcaccgatgt cgttgtccta gaacgcaata ccgtcgcgtc gggaatgacc ggcaagtcca
    12901 gcggcatcgt ccgctgccac tacggagtca cctcgctggc cgcgatggcc actgtcggac
    12961 tcgaggtgtt cgagcaggcc gaggacatct tcggcaccga catcggcttc cggcagaccg
    13021 gatacgtggt cggtgtcggc gaacccaacg tcgaatcgct gcgcaaaagc ctggccgcgc
    13081 agcgggccgt cggcgtgcag accgaagaga tcgacaaatc cgaagtcgcg cggatgtggc
    13141 cgttcgccga cctgacgccg ttcgaggcct tcgggtggga ggcgcgcggc ggctacggcg
    13201 acgcgtacat gacggcgcag gcattcgcgg cgtcggcgcg cacggccggc gttcgaatcc
    13261 gccagggcgc cacagtcacc gaactgttgc tacaaggcga tcgggtcacc ggtgtgcgcc
    13321 tcgccgacgg cgaccagatc tctgcgggca ccgtcgtcgt cgcgaccggc gtatggacga
    13381 aacccttcct cgcgccgtac ggcatcgagg tgccgatgcg cgtggtgcgt gagcagatcg
    13441 tcacgatcgc acccggccta gacattggct ccgtgcccgt cttctccgat ctggtgtcgc
    13501 tgcagtacgt ccgcccggag gtcggcggcg acatcctgtt cggcaacagc gacctgtccg
    13561 acgtcgagca aacggatccc gacaactacc tcaaccgcgc caccgacacc ttcgtcgacc
    13621 tcaccgtcga caaggtcggc actcgcttcc cgggcttcac cgatgcggca atcaccggca
    13681 gctacgcggg gtgctacgac gtcacgccgg actggaaccc ggtcatcagc cgaaccgacc
    13741 tgggcgggct tttcgtggcg gccggtttca gtgggcatgg gttcaagatc gcgcccgcgg
    13801 tgggtcggct ggtcgccgat ctcgtcgtcg acggacacag cactgacccg cggatcccgc
    13861 attccgactt ccggctgtcg cggttcgacg agggcgacct gctgaggagc ccgtatccct
    13921 atgtcggtgc cggccagatg cgatgattcc tgacaggaaa catgtctgac gttccgctac
    13981 tccgcaacaa gtccggcacc gcccgcgacc gagacccgca cgagcccgtt gacgagctcg
    14041 agatcgaggc cgcgatcggg cggaacgtgc ggcagttgcg gcagcagcaa gggctcacgg
    14101 tcgcggaaat ggctgcccgc gttggcattt ccaaggcgat gatgtccaag atcgagaacg
    14161 cccagacgtc gtgcagcctc agcacacttg cgttgctggc caagggattt gatgtcccgg
    14221 tcaccagcct gttccgcggc gccgacgtcg agcggcccgc cgcgttcgtc aaggccggca
    14281 ccggcgcgcg aatcgtccgc aacggcacca aggagggcca cgagtatcag ttgctcggct
    14341 cgttgcgcgg cgagcacaaa cggctcgaat gcctgcacgt cacgctgtcg gagaagagcc
    14401 agacctatcc gttgttccag catccgggca ccgagttcat ttacatgctc gaaggcgtca
    14461 tggactacag ccacagccgc tcggtctatc ggctgcatcc gggcgactcg ctacagatcg
    14521 acggcgaggg cgcgcatggt cccgtcgatc tggtcgagct cccgatccgc ttcctgtcgg
    14581 tgatcgcgtt cccggattct caggtgtaga gcttggccgc agccatcgcc acgtcggtga
    14641 cgacgtcgca atgctcgccg ccgctgtcga cgacaaccgc aggccggatg acgacgtcgc
    14701 cggcatcgac gtagtcgccg ccgaccagct tgcgcagcat cggggcgccg acgaacgcca
    14761 ccgccgagca gtaggtcttg tcgacgaaga tctcgacacc gtcggtttcg cggcgtgccc
    14821 gcgtctcggt ggcctcgtcg tagacctgcg gcttgagcga cacctgaatc ggaacgatgt
    14881 ctctgaaccc gctgcggaag atgccgaagc tgcattcgta ggggcgggac cgggtgatgt
    14941 cccagccgtc gatgtcggcg ccgaggacgg acatcacctc gcacggatcg cgctcggcca
    15001 ccgcggacgg ataggtcgcg acggcgtcgc ggaccggcag ttgcagcgat tcgagccgcg
    15061 gcgcgatggc ccggaccagc gcctgcgcga agtcgcagtc ctcctcggcg atcaggctga
    15121 cccgcacgaa cggctgatcg ggtttgcgca gcggctgagc gccgggcagc accgacaggt
    15181 tcagcggcac caggtaatcg
//
LOCUS       DASRJA010000382         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1427107,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000382 DASRJA010000000
VERSION     DASRJA010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427107"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2713
                     /locus_tag="VFC01_16970"
     CDS             <1..>2713
                     /locus_tag="VFC01_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81361.1"
                     /translation="TANILIAIPSRGSVSSQFRVAHDPAPELNGVDSTGRDSVIRDFR
                     SGNSFDGPAGVLRDLEAPVIVGNVSMGITAIDSNGIITVNKRQNLLGHPVYVRGRYPF
                     VGGAISSTTGFVLGPATVPTSRPLRSGDFLEQSVTVLMPDGSSEIARIRAEILQNLEV
                     GTVLNDPNFAGLGLDVNGTQGDGPTARLRVGTIRGTDSLGRPVSFIANGTPEGQDCNL
                     RRFYYEHIAFSGGGPLITDTLFRHDFLRIDPRPSLPGGAGVEPNASVSLEFSEPMDLQ
                     RVDPTKNIALTSGTMPGSTYATDLQNAKVVAGAMLPVRWSDQAGDATIVQLQPPKGFF
                     HRSNASNDVYWFHLLLGANGLGDLGGNAVDVFNDSVSNTIANWSAQLTMRAGSQENLV
                     GWHTWPFESADEDGTANGSIDVFGQFQLIDGRLAAAQTVRFSRTADQNNLGTITRAFR
                     GEACPLNSVFQPATGGRLYWQPRMVDAVGPPNVPNPYPDFQIANQAVGIVTEPHVVRG
                     SRLMMRYLEDDFSLSNRQASDMAIDVEQLYWSPFADFDVLFDVFDRYTMALAHSDKRP
                     DTDYFFFDPGPPPICRILCPTMLSSLSRTFSDNVLRGSSPVTVFEDKVYQINPNNMFR
                     SPEQVKYIGYPKFDRSYTWRDSRLVSLDANGDIIGLGGARNPGDPGNTTLINDWTSRV
                     DSPWVPSNPTPTPSGINVVDEGDFHGERRRDHDPIALPLLVDFKVFPDGTANQLASGA
                     NGFQVAMLGPPSNFLIPPQTPGGYYNFVGSGCPQADPWPFVRVHTSGSENPVDSQPPI
                     IVDPANTPVATGGFHKDAGAIIMAPQSPFSATKALVQVPPGDGMLHWARADFVRKVST
                     ITFGFFDTMRPNLHAPEVGAAPPAGYPDFLGSGSAGELRISEFTSLL"
BASE COUNT          439 a          996 c          857 g          421 t
ORIGIN      
        1 cgaccgccaa catcctcatc gcgatcccgt cgcgcggctc ggtgtcgtcg cagttccggg
       61 tcgcacacga cccggcgccc gagctcaacg gcgtcgacag caccggccgc gactccgtca
      121 ttcgcgactt ccgctccggc aacagcttcg acgggcccgc tggtgtgctg cgcgatctcg
      181 aggcaccagt gatcgtcggc aacgtgtcga tgggcatcac tgcgatcgac agcaacggca
      241 tcatcacggt caacaagcgg cagaacctgc tcggtcaccc ggtctatgtc cgcggccgct
      301 acccgttcgt cggtggcgcg atctcgtcga ccaccggctt cgtgctcggc ccggcaacgg
      361 tgccgacgtc gcggccgctg cgctcgggtg acttcctcga gcagtcggtg acggtgctga
      421 tgccggacgg ttcgtccgag atcgcacgca tccgcgccga gatcctgcag aacctcgagg
      481 tcggcaccgt gcttaacgac ccgaacttcg cgggcctggg cctcgacgtg aacggcacgc
      541 agggcgatgg tccgacggcg cgcctgcgcg tcggcacgat ccgcggcacc gactcgctcg
      601 gccgcccggt cagcttcatc gccaacggga cccccgaagg ccaggactgc aacctgcgcc
      661 gcttctacta cgagcacatc gcgttcagcg gcggtggccc gctgatcacc gacaccctgt
      721 tccggcacga cttcctgcgc atcgacccgc gaccgtcgct gcccggcggc gccggcgtcg
      781 agcccaatgc ctcggtctcg ctcgagttct ccgagccgat ggacctgcag cgcgtcgacc
      841 cgaccaagaa catcgcgctg accagcggga cgatgccggg ctccacctac gcgaccgatc
      901 tgcagaatgc caaggtggtc gcgggcgcca tgctgccggt ccgctggtcg gatcaggccg
      961 gcgacgccac gatcgtccag ctgcagccgc cgaagggctt cttccaccgc agcaacgcgt
     1021 ccaacgacgt ctactggttc cacctgctgc tcggcgccaa cggcctcggg gacctcggcg
     1081 gcaacgcggt cgacgtgttc aacgacagcg tctcgaacac gatcgcgaac tggtcggcgc
     1141 agctgacgat gcgggccggc agccaggaga acctcgtcgg ctggcacacc tggccgttcg
     1201 agagcgcgga cgaggacggc acggcgaacg gctcgatcga cgtgttcggc cagttccagc
     1261 tgatcgacgg tcgtctggcc gcggcgcaga cggtgcggtt cagccgcact gccgaccaga
     1321 acaacctcgg caccatcacg cgtgcgttcc gcggcgaagc ctgcccgctg aactccgtgt
     1381 tccagccggc caccggcggc cggctctact ggcaaccgcg catggtcgac gccgtcggac
     1441 cgccgaacgt gccgaacccg tacccggact tccagatcgc gaaccaggcg gttggcatcg
     1501 tcaccgagcc gcacgtcgtg cgcggttcgc gcctgatgat gcgctatctc gaagacgact
     1561 tcagcctgtc gaaccgccag gccagcgaca tggccatcga cgtcgagcag ctgtactggt
     1621 cgccgttcgc ggacttcgac gtgctgttcg acgtgttcga ccgctacacg atggcgctcg
     1681 cgcactcgga caagcgcccc gacaccgact acttcttctt cgatccgggt ccgccgccga
     1741 tctgcaggat cctgtgcccg acgatgctca gcagcctgtc gcgcacgttc tcggacaacg
     1801 tcctgcgcgg ctcgtcgccg gtgaccgtgt tcgaggacaa ggtctaccag atcaacccga
     1861 acaacatgtt ccgctcgccg gagcaggtga agtacatcgg ctatccgaag ttcgaccgca
     1921 gctacacgtg gcgcgactcg cgcctggtgt cgctcgacgc gaatggcgac atcatcggac
     1981 tcggcggtgc gcgcaacccc ggtgacccgg gcaacaccac gctcatcaac gactggacgt
     2041 cccgcgtcga ctcgccgtgg gtgccgagca acccgacgcc gacgccgtcg ggcatcaacg
     2101 tcgtcgacga aggcgacttc cacggcgagc gacgccgtga ccacgacccg atcgcgctgc
     2161 cgctgctggt cgacttcaag gtgttcccgg acggcaccgc gaaccagctg gcgagcggcg
     2221 ccaacggctt ccaggttgcg atgctcggcc cgccgagcaa cttcctgatc ccgccgcaga
     2281 caccgggcgg ctactacaac ttcgtcggct cgggctgccc gcaggccgat ccgtggccgt
     2341 tcgtgcgcgt gcacaccagc ggcagcgaga acccggtcga cagccagccg ccgatcatcg
     2401 tcgacccggc gaacacgccg gtcgcgaccg gcggcttcca caaggacgcc ggtgcgatca
     2461 tcatggcgcc gcagtcgccg ttctcggcaa ccaaggcgct ggtccaggtg ccacccggcg
     2521 acggcatgct gcactgggcg cgcgccgact tcgtgcgcaa ggtctcgacg atcacgttcg
     2581 ggttcttcga cacgatgcga cccaacctcc acgcccccga ggtcggtgcg gcgccgccgg
     2641 cgggttaccc cgacttcctc ggcagcggct cggcgggcga gttgcgtatc tcggagttca
     2701 cgtcgctgct cga
//
LOCUS       DASRJA010000383         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_651270,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000383 DASRJA010000000
VERSION     DASRJA010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651270"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..1735
                     /locus_tag="VFC01_16975"
                     /pseudo
     CDS             321..1735
                     /locus_tag="VFC01_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
     gene            complement(1847..>2243)
                     /locus_tag="VFC01_16980"
     CDS             complement(1847..>2243)
                     /locus_tag="VFC01_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81362.1"
                     /translation="GSRFGSDLEDPSMCHSLLDVDVKNFFSGDGTVKVSITQAEALTA
                     ASFGIPLFTFVDAAVYHDYFVYQQNKDKAFATEISYPSISQPRTAEHIFRFINFLESR
                     SYNNAVITFERLEDVVGHLSPDPRMKVAC"
BASE COUNT          407 a          770 c          692 g          374 t
ORIGIN      
        1 gccggttccg taacgagatc ggcttcctgc taggccccgg ccggcgtaag ggtgtcatcc
       61 caattgttga tcatgacctg agcggtgcgg ggaaaggtct ggtacgtgat gccgctcgcc
      121 gaaccgatcc gcgacgccct cggcatcgac cccgacccag accgagtggt ggaggcaatg
      181 gcagcaatcg ccctctaagc cctgatccac ggacgaaagg tggtttgagg gctgtgtcgt
      241 ggacgtgatg atgccctctg aactggcagg atcgaagttc ttcacgcttt gatcgtccgg
      301 ttcgagaggg caccgtcgag gtgagattag cgcatgcttc cggcgcagtg cgtgcgtcgt
      361 ttgatgatcc gaatgtggtg tcgcaggccg ggctggtgcc ggtgatgcga ctggcccagt
      421 cctgcgggct gcacacgatg gtcgccgagc gggtccgggt gccggcacac cgggggtcca
      481 atgcggccgg caaggtcgcc acgatcgtgg ccgggatgct gaccggggcc gactcgatcg
      541 atgatctggg cgcggtgcgc cacggcggga tgcggcagct gttcggcgag gtgtacgcgc
      601 cgtcgacgct gggcgagttc ttgcgcacgt tcacccatgg ccatgtccgt caactccagg
      661 ctgtgacacg gcagttgctg gtccggctcg ctcgccgcac gtcgttgctg cccggcgccg
      721 aagcggtgac gttcgtcgac gtcgactcgt tgctgcgccg ggtgtacggc aaacgcaagc
      781 agggcgccgg tttcggccac gccaaggtgg gcgggtacca ggtgctgctg cgtgggctga
      841 accccctgat cgccacgatc agcacccccg atgcggcacc ggtgatcgcc gcgacccagc
      901 tgcgggccgg caacgccggc tcggcccgag gcgccgcctc gctggtggcc gaggccatcg
      961 ccacgaccaa ggccgtcctg gacacccaag cccagaacca ggctgaggtc ccgggcgaga
     1021 tcgtggtgcg ggcggactcg gcgttctact cccgcgcggt cgtcgccgcc tgccgccgcg
     1081 cccgagtgcg gttctcgatc accgtgcgca tcgacgccaa agtgcgccgg gcgatcgcca
     1141 cgatccccta cgacgcctgg acggaaatcc gctaccgcaa ccgatcttcg acgacgagca
     1201 acaccgcttc atctcccgcg cgcacctcgc cgagaccacc tacaccgcgt tcgagggcac
     1261 ccgcgacgag acgaccgccc ggctgatcgt gcgccgcgtc cccgacctca acacggccac
     1321 catcgacgcc cagggcgagt tgttcaccgt ctggcgctac cacgccgcgg tcaccgacag
     1381 cccgttcatc ctcgtgcagg ccgaggccca gcaccgcggt cacgccatca tcgaacaggt
     1441 cttcgccgaa ctcatcgacg gaccgctggc gcacctaccc tcgggccggt tcaccgccaa
     1501 caacgcgtgg ctgtcctgca ccgcgatcgc gcacaacctc acccgcgccg caggcgtcct
     1561 cgctggccga cgccacggca ccgcccgcgc cgctaccatc cgccgacacc tgatcaacgt
     1621 cgccggccgg atcgcccacc acgcccgtgc cgtcaccatc cacctacccg aacactggcc
     1681 ctggcaacac gagtggctcc ggctgttcca ctgcagccac gccccaccga cctgatcgtc
     1741 cataaacgac acgcggcggc cgagcggcca accccgaaca acctcaccaa cgaccggagc
     1801 gaaccggcga ccgggctggt cgatcaacca cgcccgcacc caaacatcaa cacgcgacct
     1861 tcatccgtgg atcagggcta aggtgcccaa caacgtcctc aagtcgctcg aaggttatga
     1921 ctgcgttgtt atacgagcga ctttccagga aattaataaa cctgaagata tgttcagcgg
     1981 tgcgcggctg actgattgag ggatatgaga tctcagttgc gaacgctttg tctttattct
     2041 gttggtagac gaagtaatca tgatagacgg ccgcgtcgac gaaggtaaaa agtggaatgc
     2101 cgaaggatgc agccgtcaag gcctctgctt gagtgatcga gactttcacg gttccgtcgc
     2161 cgctgaagaa gtttttcaca tcgacatcca acaatgagtg acacatgctg ggatcttcaa
     2221 ggtcactgcc gaaccgggag cct
//
LOCUS       DASRJA010000384        23164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3670250,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000384 DASRJA010000000
VERSION     DASRJA010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 23164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23164
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3670250"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFC01_16985"
     CDS             <1..495
                     /locus_tag="VFC01_16985"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN81363.1"
                     /translation="AADAGVTVTFDVADATKLTGYDGRFDTVVDSALYHCLDEDGQQA
                     YIAGLYRATRPGARLHLWCFSDGNINGVIAPMGSISESNLRDTLTANGWRIDFLGPTT
                     YLGNALAFGGDREFPEALAEQMPPEAVEQMRAVTERFEKIAEFLDGDRVYLPFTVVHA
                     HRVD"
     gene            592..1986
                     /locus_tag="VFC01_16990"
     CDS             592..1986
                     /locus_tag="VFC01_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HZN81364.1"
                     /translation="MSHRITDWDPEDVVAWEAGNKRIARRNLIWSVAAEHIGFSVWSI
                     WSVMVLFMPEAIYGFSAGDKFLLGATATLVGACLRIPYTLATATFGGRNWTVFSAFVL
                     LIPTVGTMWLLAHPGLPLWPYLVCAALAGFGGGNFASSMTNINAFYPQRLKGWALGIN
                     AGGGNIGVPMVQIVGLLVIATLGNRQPYWVCAVYLVALVIAGIGAALYMNNLEQHKID
                     VTAMKAILRERDTWVISLLYIGTFGSFIGFAFAFGQVLQINFAAGGQSAAQASLHAAQ
                     IAFIGPLLGSVSRVYGGRLADRIGGGRVTLAVFAGMILGAGILVGVSTLDDHTKGAAT
                     GAMMAGYVVGFIALFLLSGIGNGSVYKMIPSIFEARSRSLEISEAERVAWSRAMSGAL
                     IGFAGAVGALGGVGINLALRQSYLTSHSATSAFWIFLAFYVVACAVTWVMYVRRPVSA
                     REVTAEPVADLART"
     gene            2107..3255
                     /locus_tag="VFC01_16995"
     CDS             2107..3255
                     /locus_tag="VFC01_16995"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZN81365.1"
                     /translation="MTEPDWAPLTGFRVAVTSARRADELGALLQRRGATVTSAAAIQM
                     VPLPDDDELRAHTEALIDVPPDIVIATTGIGFRGWVAAADGWGLTHELLTALGKARVV
                     SRGPKATGALRAAGLPEEWSPESESSRELLHYLVEGGVAGQRIAVQLHGATDDWDPFP
                     EFLDELRAAGAEVVPIRVYRWHPAPRNGEFDQLVAGIADEKFDAVAFTSAPAVASVLM
                     RAAEMGIGDQVLSAFRTGVHAMCVGPVTARPLVRLGVPTSAPERMRLGALARHITDEL
                     PLLQSRTVRVAGHLLEIRGTCVLVDGVVKAISPAGMATIRALAHRPGAVVSRTDLLGA
                     LPGSGTDTHAVETAVLRLRTALGDKNIVSTVVKRGYRLAVDDELAHAL"
     gene            3252..3932
                     /locus_tag="VFC01_17000"
     CDS             3252..3932
                     /locus_tag="VFC01_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HZN81366.1"
                     /translation="MSLVLVAHGTRKQRGVAMVGDLASRVAATLGQTVHVSFVDVLGP
                     TPSEVLAPMAGPTIVVPAFLSRGYHVTADIPAHVEASGHRDVTVTEALGPGPQLVRVL
                     ADRLIESGWRRDDSVILAAAGTSDPGAQRDLHTMATWLSAITDSRVELAFAATWTPRV
                     HDAVAALRRRGARRVVVASYLLSEGLFQDRLRASGADVVTEPLGTHPGLVRLIANRFS
                     RARYSVAA"
     gene            complement(3901..4047)
                     /locus_tag="VFC01_17005"
                     /pseudo
     CDS             complement(3901..4047)
                     /locus_tag="VFC01_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890740.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
     gene            complement(4148..4495)
                     /gene="nirD"
                     /locus_tag="VFC01_17010"
     CDS             complement(4148..4495)
                     /gene="nirD"
                     /locus_tag="VFC01_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HZN81367.1"
                     /translation="MTLLNDVAVWTTACAYDFLIPNRGVGVLLPDGAQAALFRLDDGS
                     LHAVGNIDPFSGAAVMSRGIVGDRGGRATVQTPIKKQAFALDDGSCLDDPEVSLPVYA
                     TRITADGIVEVGA"
     gene            complement(4502..7051)
                     /gene="nirB"
                     /locus_tag="VFC01_17015"
     CDS             complement(4502..7051)
                     /gene="nirB"
                     /locus_tag="VFC01_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HZN81368.1"
                     /translation="MRSKSSVVVVGHGMVGHRFVEALRSRDGDGAWRVTVLAEEADAA
                     YDRVGLTGYTEHWDRGLLALPGNDYPGDELVELRLGSRVAEIDRSAKAVVTANGDRVH
                     YDALVLATGSYAFVPPVPGHDLPPCHVYRTLDDLDAIRADAMRAVGGPAPVGVVIGGG
                     LLGLEAANALRQFGLQTHVVEMAPRLMAQQLDEAGGALLGRMIGELGITVHVGVGTQA
                     IEPAQRNRPVRRSADHDAVRVTLSDGTAIDAGVVVFAAGVRPRDELARAADIDVAERG
                     GVLTDLSCATRDPNIYAIGEVAAIEGRCYGLVGPGYTSAEVVADRLLGGAAEFPEADM
                     STKLKLLGVDVASFGDAMGSTPSCLDVVVNDPVNQTYAKLVLSDDAKTLLGGILVGDA
                     SSYGVLRPMVGEPLPGDPMALIAPAGSDGGSSTLGVGALPAAAQICSCNNVTKGDLCD
                     AIAGGCSDVPSLKSCTKAGTSCGSCVPLLKQLLEAEGVEQSKALCEHFSQSRAELFSI
                     VAATSIRTFTALIERFGTGKGCDICKPVVASILASTSSEHILDGEQASLQDSNDHFLA
                     NIQKNGSYSVVPRSPGGEITPEQLILIGEIARDFNLYTKITGGQRIDMFGARVDQLPE
                     IWRRLVDGGMESGHAYGKALRTVKSCVGSTWCRYGQQDSVNMAVEIEKRYRGLRAPHK
                     IKMAVSGCARECAEAQSKDVGVIATEQGWNLYVCGNGGMSPRHAQLLASDLDDETLVR
                     YIDRFLMFYIRTADRLQRTAPWLEAMEGGLDHLRDVVCNDSLGLAAEFEEAMERHVAG
                     YACEWKGVLEDPNKLSRFVSFVNAPDVVDPTVKFTEESGRIVPIGMPKVRG"
     gene            7257..7664
                     /locus_tag="VFC01_17020"
     CDS             7257..7664
                     /locus_tag="VFC01_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZN81369.1"
                     /translation="MSNVTLWARPAWEIDRWVKQGVFNGFSPAAEVAKDGDDAVVRVE
                     LPGVDVEKDVNVEVDRGQLVIRGERRDERSESSDGRTLSEVRYGSFRRTFKLPAHVTS
                     DAISASYDAGVLTVRVAGAHKGAEAQRIAIENK"
     gene            complement(7715..8467)
                     /locus_tag="VFC01_17025"
     CDS             complement(7715..8467)
                     /locus_tag="VFC01_17025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN81370.1"
                     /translation="MTDDMWGTGDYRAVAEKVTSIADVLVPRANIEPGMDVLDVACGT
                     GNASIPAARLGARVTGLDFSPGLIAIARQRGAEAKVDVDWLEGDAQSLPFDDGSFDRV
                     ISAIGHMFAPDHKRTADELRRVCRPDGRIAIACWTPVGAIGEMFKRLGAISPAPPAGF
                     QPPVLWGTEEHVRELLGDSVEFERHNVEFDEPSPESYVDFMLTSFPPLIAMRAELGDE
                     LVRETYLDWAVDVNEADDGTMRYRGEYLVSVT"
     gene            complement(8528..9028)
                     /locus_tag="VFC01_17030"
     CDS             complement(8528..9028)
                     /locus_tag="VFC01_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HZN81371.1"
                     /translation="MTRVDVLTGVEFDEFLRAFEDAAPSFDPVPMQRIVDSGGTWDEV
                     RAKVADNAPNELMVYAKIDATPLLGVAGHDVKAVEYLVGNHVIAETMFRHDPRALLYA
                     PLRLLVYSDPDGNAVFSMDQPSSAFASLGISEVTEVGLSLDRKVANLLRVIGVDTGDA
                     FGVEEN"
     gene            complement(9069..9443)
                     /locus_tag="VFC01_17035"
     CDS             complement(9069..9443)
                     /locus_tag="VFC01_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81372.1"
                     /translation="MSPDDDEPSWHDQQPVIVAAGVAGLLLLALLVWAVIRTADNSSG
                     PPDPVEFPTSSATSSTYTTESTSSTSYAVPSVQTSQDNPALTRPPESTAPDDGPTDDE
                     TTTSTTTTNPYLTTTPTNAGHI"
     gene            complement(9454..10212)
                     /locus_tag="VFC01_17040"
     CDS             complement(9454..10212)
                     /locus_tag="VFC01_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZN81373.1"
                     /translation="MSDQVLKQRAEALLALHQPGNPVVLPTVWDAWSARLAVDAGFVA
                     LTVGSHPMADSVGKADQEGMTFDEVVTRVKQITAAVDVPVSVDIESGYGQSPTRLITG
                     LLDAGAVGLNIEDTVHSEGGRLRSSSEHAELVGALRKAADATGVHVVINARTDLFLRQ
                     DGDEADRVDRAIARLKEAATAGADSLYPVGRHDPDTLRRLAAELPLPINAIALPDQDD
                     PASFGPLGVGRVSFGPFWQFALSAYSKEMLARWA"
     gene            10324..10611
                     /locus_tag="VFC01_17045"
     CDS             10324..10611
                     /locus_tag="VFC01_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81374.1"
                     /translation="MSTKTELAELHELIGSMRRCVTALASKYGDSPATRRIVNDAERI
                     LNDIDRLDIDAEELELGSGVTRHHGGEKIAIPDTPHETEFWGDEDDRGVAG"
     gene            10654..11475
                     /locus_tag="VFC01_17050"
     CDS             10654..11475
                     /locus_tag="VFC01_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81375.1"
                     /translation="MSAPTADRKATGVFSPSRAQIPQRTLRTDRWWLPTLWSNLGFAA
                     FLLYGLVRASWQSAYWVEDYHYLTPFYSPCISASCAPGSSHLGVWLPEFPPLVPLAII
                     TLPFLLLFRLTCYYYRKAYYRSVWQSPPGCAVAEPHSTYTGETRLPLIIQNTHRYFFY
                     AAVLISVLNTYDAIVAFHSPSGFGFGLGNIVLTGNVILLWIYTLSCHSCRHIVGGKLK
                     HFSKHPVRYWFWNQVSLLNNRHMMWAWITLGTLVLTDFYIMLVASGTISDLRFIG"
     gene            11516..13441
                     /locus_tag="VFC01_17055"
     CDS             11516..13441
                     /locus_tag="VFC01_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HZN81376.1"
                     /translation="MAEVERHQYDVVVIGAGGAGLRAVIEARERGLKVAVVCKSLFGK
                     AHTVMAEGGCAAAMGNANPKDNWQVHFKDTMRGGKFLNNWRMAELHAKEAPDRVWELE
                     TYGALFDRTKDGRISQRNFGGHTYPRLAHVGDRTGLEIIRTLQQKIVSLQQEDKAEFG
                     DYEARIKVFHECTITDLIKDGERIAGAFGYWRETGRFILFEAPAVVLATGGIGKSYKV
                     TSNSWEYTGDGHALALRAGATLINMEFVQFHPTGMVWPPSVKGILVTEGVRGDGGVLK
                     NSDGKRFMFDYIPPVFKGQYAESIEEADQWLKDNDSARRTPDLLPRDEVARAINSEVK
                     GDRGTPHGGVYLDIASRLPAEEIKRRLPSMYHQFMELAEVDITKEPMEVGPTCHYVMG
                     GVEVDPDTGAAKTPGLFAAGECSGGMHGSNRLGGNSLSDLLVFGRRAGLGASDYVRAL
                     SERPQVSEDAVAEAAKLALAPFDGPTNGDSAENPYTLQLDLQDTMNTLVGIIRKADEV
                     SEALDKLNELRERFKRVQVEGHRQFNPGWHLAIDLRNMLLVSECVAKAALERTESRGG
                     HTRDDHPSMDPEWRKTLLVCRAIDDGADVSVVPDVDITREDQVPMREDLLELVDIEEL
                     EKYFTPEELADHPARRS"
     gene            13441..14190
                     /locus_tag="VFC01_17060"
     CDS             13441..14190
                     /locus_tag="VFC01_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HZN81377.1"
                     /translation="MAYQAKMRVWRGDDDGGALQDYAVEVNEGEVVLDIIHRLQQTQT
                     PDLAVRWNCKAGKCGSCSAEINGRPKLMCMTRMSTFDPTETITVTPLRTFPVIRDLVT
                     DVSFNYEKAREIPSFTPPKDLQPGEYRMMQEDVNRSQEFRKCIECFLCQNTCHVVRDH
                     EENKQAFAGPRYLMRQTELDMHPLDTLARRPEQAQEENGLGFCNITKCCTEVCPEHIK
                     ITDNALIPLKERAVDKKYDPVVWLGNKLFRR"
     gene            14292..14447
                     /locus_tag="VFC01_17065"
     CDS             14292..14447
                     /locus_tag="VFC01_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81378.1"
                     /translation="MREPHSINDIRTAIRELTTRAELARKEGRPADAEELEQRIQSYR
                     DELAARP"
     gene            complement(14448..14936)
                     /locus_tag="VFC01_17070"
     CDS             complement(14448..14936)
                     /locus_tag="VFC01_17070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZN81379.1"
                     /translation="MMSSTDLTPTTLREAFGHFPSGVIAIAAEVDGTLVGLAASTFVP
                     VSLDPPLVSFCVQNISETWPKLKGLPYLGISVLGEAHDDAARTLAAKTGDRFAGLETV
                     SRESGAVFVKGTSVWLESAIEQLVPAGDHTIVILRVSDITVHADVPPIVFHRSTFRRL
                     GA"
     gene            complement(14998..16089)
                     /locus_tag="VFC01_17075"
     CDS             complement(14998..16089)
                     /locus_tag="VFC01_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN81380.1"
                     /translation="MLRVAVWGTGNMGATAIRSATAFPGLQLSGVITSSPDKEGRDAA
                     TFARLDSETGVLATTDVDAALAGCDAVAYMSSGDIRPDEAVDEIVRCLRAGKHVVTPS
                     LYSLYDPRSAPPEWVQRLTAAAEEGGATLLVSGVDPGWGNDALAVIAAGLCTRIRTIN
                     CQEIFDYSTYNQPYAVKVSCGFGSAMGREDPVPMMLLPSIPTMVWGGNIRLIGRGLGL
                     EIDEITEEVERLPLDESVDTVMGRFEKSTQGAFWLKVIGKSKGRERVIIDHITRIHAS
                     CAPDWPYPDEGVGDHRVIIDGDPQLTLTSRADVPGGTRADGGNTTAANRLLGALHWLA
                     EQKPGIYDGLDVPMQSTLPPEVEASRWAD"
     gene            16328..18163
                     /locus_tag="VFC01_17080"
     CDS             16328..18163
                     /locus_tag="VFC01_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN81381.1"
                     /translation="MSHYKSNVRDQVFNLFEVLGVDKTLGQGDYADLDVDTAKEMLAE
                     MARLAEGPVAESFVEGDRNPPTFDPNTHSVTLPDAFKKSVNAVLEAGWDRVGLDEALG
                     GTPTPKALMWALNEHILGANPAVWMYAGGAGFAQIVYHLGTEEQKKWAVIAAERGWGS
                     TMVLTEPDAGSDVGAGRTKAVKQDDGSWHIDGVKRFITSGDSGDLFENIFHLVLARPE
                     GAGPGTKGLSLFFVPKFLFDFETGEIGERNGVFVTNVEHKMGLKVSATCELSLGQHGV
                     PAKGWLVGEVHDGIAQMFDVIEQARMMVGTKAIATLSTGYLNALEYAKERVQGADMTQ
                     MTDKTAPRVTITHHPDVRRSLMTQKAYAEGLRALYLFTATYQDTAVAKAVHDVDAELA
                     VKVNDLMLPIVKGVGSEQAYAKLTESLQTFGGSGFLQDYPIEQYIRDAKIDSLYEGTT
                     AIQAQDFFFRKIIRDKGAALAYVAGQIEQFVKSESGNGRLKAERALLATALEDVQGMA
                     ATLTGYLMAAQENPAELYKVGLGSVRFLMSVGDLVIGWLLGRQAAVAVDALDKGASGD
                     DRAFYEGKVAVASFFAKNFLPLLTSTRTVIENLDNEVMELDEAAF"
     gene            18236..18887
                     /locus_tag="VFC01_17085"
                     /pseudo
     CDS             18236..18887
                     /locus_tag="VFC01_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063995.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
     gene            19052..20803
                     /locus_tag="VFC01_17090"
     CDS             19052..20803
                     /locus_tag="VFC01_17090"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HZN81382.1"
                     /translation="MTTLSPPNKRLSKRAKMVIVVAAAFLVAVAAVASIIAVVGHQTL
                     EKKTVSYSSQVALPFTNLKIPVGIAVDAAGSAYVADYYDSIVWKLPPGAEVPIALPFN
                     DIKHPTGVALDTSGNLYVTDGDNNRVLKLAPGASDATVMPFTGLKNPTGVAVDTAGNL
                     YVADFNNSRVLKLSAGANAPEVLPTKGLKYPEGLAIDTAGNLYIADKGNRRVLKLTAG
                     AKAPEVLPFTGLKDADGVAVDGKGNVYVADQASRKWWMLAAGSHSQTEMPSEYIGGPF
                     GVAVDAGGTLYVTDAGNGTVHKLAAGANKLTVLPFNNVSTPWGAAVDASGNLYIAESE
                     HVLKLAAGASAPAVLPLPGVRAPDAVAVDTAGNLYVVEPDSQRYIENNNPKVWKLAAG
                     ATEPTLLPFPGLKAPSAVAVDTAGNLYVTDMYKNRVWKLAEGANNPIALPFKDLENPA
                     GVAVDTAGNVYVADRDTKHVYELAAGANSATVMPLPGLERLDAIAVDSAGNLYITDAH
                     DPCTHSGPCTFIDETGRVEEGRVLKFATNSTVPTVLPFDGLDYSRGIAVDAAENVYVV
                     DGSDRVLKLPVMQGEDE"
     gene            complement(20807..21454)
                     /locus_tag="VFC01_17095"
     CDS             complement(20807..21454)
                     /locus_tag="VFC01_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN81383.1"
                     /translation="MTRLVIAEDSAILRDGLVQLLLERGHEVVAKVGTADELIEAVNA
                     FTPDVAVVDIRMPPTFTDEGLVAAVALRRSHPDVGVLLFSQWVETRYATELLAGNPEG
                     VGYLLKDRVTDIAEFDAALRRVASGGTALDPEVVRQLIGSRRQATVLDRLTAREREVL
                     ELMAEGHSNSALAQHLSITERAVEKHVSAIFTKLDLPPSQAHHRRVLAVVTYLNS"
     gene            complement(21451..22722)
                     /locus_tag="VFC01_17100"
     CDS             complement(21451..22722)
                     /locus_tag="VFC01_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HZN81384.1"
                     /translation="MTTAVATQSDWTRTLLVPLRAPAWRAYLYFVLVSVLAVVGVAFI
                     FFAGLASGLLMATLVGIPLLALVVMSGRAWNRLYRSLARLLGTTIDAPPPFVRPAGRL
                     HTVAAALTDGVGWRSLGFLALHAVLMTPIGYFVLVGVAMSGALVVSPVVWAALGEPFV
                     TLGEPVDSLPAYLLMSAGGVVALYGLGWLMLGLGRAHVWVAGALLGPTERERRVGELE
                     RARADAVDDPAATLRRVERDLHDGTQARLITVAMALARAEEHFAASDVTRGRELVSDA
                     LANTKDTLTELRDVIRGIRPPALDLGLGEAVQTLAARNPIPVEVAVDVATRPSIGVET
                     MAYFCVAELLANVSRHSGASHASVRIHGNADELRIIVEDNGSGGAQVGNGSGLTGLRD
                     RLAMLDGRLEIDSPPGGPTVVTVVVPPGTEQ"
     gene            complement(22971..>23164)
                     /locus_tag="VFC01_17105"
     CDS             complement(22971..>23164)
                     /locus_tag="VFC01_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN81385.1"
                     /translation="RAMLDREGYAGPEDAAIIGDEKTVSDRLDELAAAGVDEFAAVTF
                     DPSADGRERTRALLRTRDK"
BASE COUNT         4038 a         7585 c         7578 g         3963 t
ORIGIN      
        1 gcggccgacg ccggtgtcac ggtgacattc gacgtcgccg acgcgacaaa gctgaccggg
       61 tacgacggtc gattcgacac cgtcgtcgac agcgcgctgt atcactgcct cgacgaagac
      121 ggccagcagg cctacatcgc cgggttgtat cgcgcgacgc ggcccggcgc ccggttgcac
      181 ctgtggtgct tctccgacgg caacatcaac ggtgtgatcg cgccgatggg ctcgatatcg
      241 gagtccaatc tccgcgacac gttgacggcc aacggctggc gcatcgactt tctcggtccc
      301 accacgtatt tgggcaacgc cctcgcgttc ggtggtgacc gcgagtttcc cgaggctctt
      361 gccgagcaga tgccgcccga ggctgtggag cagatgcggg cagtgaccga gcggttcgag
      421 aagatcgcag agtttctcga cggcgaccgg gtctatttgc cgtttaccgt ggtgcacgcc
      481 caccgcgtcg actgaacctc acatctgccg tttcagctac gtgagcggaa gttcacggcg
      541 gggttatcgc gaggcgtttt cgcgcaataa agcgttccta gcgtgggcgg catgtcgcat
      601 cgcatcacgg actgggatcc cgaggacgtc gtcgcatggg aagccggtaa caagcgcatc
      661 gcccgccgca acctgatctg gtcggtggcg gcggagcata tcggcttctc ggtgtggtcg
      721 atctggtcgg tgatggtgct gttcatgccg gaggcgatct acggcttctc ggccggcgac
      781 aagttcctgc tcggcgccac cgcgacgctg gtcggcgcct gcctgcggat tccgtacacg
      841 ctggccacgg ccacgttcgg cggccgcaac tggaccgtgt tctccgcgtt cgtgctgctg
      901 atcccgaccg tcgggacgat gtggctgctg gcccatcccg gcctgccgct gtggccgtat
      961 ctggtgtgcg ccgcgctggc cggcttcggc ggcggcaact tcgcgtcgtc gatgacgaac
     1021 atcaacgcgt tctacccgca gcggctcaag ggttgggcgc tgggcatcaa cgcaggcggc
     1081 ggcaacatcg gagtgccgat ggtccagatt gtcggcctgc tcgtcatcgc caccctcggc
     1141 aaccggcagc cctattgggt gtgcgcggtg tacctggtcg cgctggtgat cgccgggatt
     1201 ggcgccgcgc tctacatgaa caacctggag cagcacaaga tcgacgtcac cgccatgaaa
     1261 gcgatcctgc gggagcgcga cacctgggtg atctcgctgc tctacatcgg cacattcggc
     1321 tcgttcatcg gcttcgcctt cgcgttcggc caggtgctgc agatcaactt cgccgcgggc
     1381 gggcagagcg ctgcacaagc gtccctgcac gccgcgcaga tcgcgttcat cggaccgctg
     1441 ctcgggtcgg tctcgcgggt gtacggcggc aggttggccg accgcatcgg cggtggccgg
     1501 gtcacgctgg cggtgttcgc ggggatgatc cttggcgccg ggattctggt cggggtcagc
     1561 acgctggacg accacaccaa gggcgccgcg acgggggcca tgatggccgg ctacgtggtc
     1621 ggcttcatcg cgctgttcct gctgtcgggc atcggcaacg ggtcggtcta caagatgatc
     1681 ccgtcgatct tcgaggcccg tagtcgctca ctggagatct ccgaagcgga gcgcgtcgcc
     1741 tggtcacggg cgatgtccgg cgcattgatc ggcttcgccg gtgccgtcgg cgcgcttggc
     1801 ggggtcggca tcaatctggc gctgcggcag tcgtatctga ccagtcactc cgcgacctct
     1861 gccttctgga ttttcctggc cttctatgtg gtggcctgcg ctgtgacctg ggtgatgtac
     1921 gtgcggcggc cggtgtcggc ccgcgaggtc actgcagaac ccgtggcgga cttggcccgg
     1981 acgtgacctc cttcgtgtgg gtcagggctg ctgggcaaca acggcgttgc ccagctcaca
     2041 tcgggcgaca cggttaggca atggcggggt aatgggttgg aaacacgctc cgcgaacact
     2101 ggcaacatga cggagcccga ctgggcaccg ctcaccgggt ttcgggtggc ggtgacctcc
     2161 gcccgacgtg ccgacgaact gggtgcgctg ctgcagcgcc gaggcgccac cgtgaccagt
     2221 gccgcggcta tccaaatggt gccgctcccc gacgacgacg aactgcgcgc gcacaccgag
     2281 gcattgatcg acgtgccccc cgacatcgtg atcgccacca caggcattgg ctttcgcggc
     2341 tgggtcgccg cggccgacgg atgggggctg acgcacgagc tgctgacggc tttgggcaag
     2401 gcccgcgtcg tgtcgcgggg tccgaaggcc accggggcgc tacgtgcggc gggactgccc
     2461 gaagagtggt cgccggagtc cgagtcgtca cgtgaactcc tgcactatct ggtcgagggc
     2521 ggcgtcgcag gccagcgcat cgcggtgcag ctgcacggcg ccaccgacga ctgggatccg
     2581 ttccccgagt ttctcgacga attgcgcgcg gcgggtgcgg aagtggtgcc gatccgggtg
     2641 taccgctggc atcccgcgcc gcgcaacggc gaattcgacc agctggtggc cggcatcgcc
     2701 gacgagaaat tcgacgccgt cgccttcacg tcggcgcccg cagtcgcgtc ggtgctgatg
     2761 cgcgcggccg agatgggcat cggggaccaa gtgctttcgg cgttccggac cggcgtgcac
     2821 gcgatgtgcg tcgggccggt tacggctcgc ccgctggtgc ggttgggcgt gccgacgtcg
     2881 gcgcctgagc gaatgcggtt gggcgcgttg gcccgtcaca tcaccgacga gctgccgctg
     2941 ctgcagtcgc gcacggtgcg ggtggccggg catctgttgg agatccgcgg cacctgcgtg
     3001 ctggtcgacg gcgtggtcaa ggcgatttcg ccggcgggga tggcgaccat tcgagcgctg
     3061 gcgcatcgtc ccggcgcggt ggtgtcgcga accgacctgc tcggcgcgct gccgggcagc
     3121 ggcaccgaca ctcacgccgt cgaaactgcg gtgctacgcc ttcgaacggc attgggcgac
     3181 aagaacattg tgtctacagt ggtgaagcga ggatatcggc tggcggtcga cgacgagttg
     3241 gcgcacgcac tgtgagcttg gtgttggtgg cgcatggcac ccgcaagcag cggggtgtcg
     3301 cgatggtcgg cgatctggct tcgcgagtgg ccgcgacact cggtcagacg gtgcatgtgt
     3361 cgtttgtcga cgtactcggc ccgacgccgt ccgaggtgct ggcgccgatg gcggggccga
     3421 cgatcgtcgt accggcgttc ctgtcgcgcg gctatcacgt caccgctgac atcccagccc
     3481 acgtcgaggc gagcggtcat cgcgatgtca ccgtgacgga agcgctcggg ccggggccgc
     3541 agctggtgcg ggtgctggcg gatcggctta tcgaatccgg ttggcgccgc gatgattcgg
     3601 tgattctcgc cgcggcgggc acctctgacc cgggcgcgca gcgcgacctg cacacgatgg
     3661 ccacatggct ctccgcgatc accgattcgc gggtcgagtt ggcgtttgcc gcgacatgga
     3721 cgccacgcgt gcacgacgcg gtggccgcgc tccggcggcg tggcgctcgc cgggtcgtgg
     3781 tggcgtcgta tctgttgtcg gagggcctgt ttcaggaccg gctgcgggcc agcggcgccg
     3841 atgtggtgac cgaacccctc ggcacgcatc ccgggttggt acggctgatc gcgaaccgat
     3901 tcagccgtgc ccgctattcg gtggcagctt agcgaccagt ggggtatcga cgccgacccg
     3961 tcccagcgcg gtcgcaccac ccggtgatcc gagcggggcg tcgcgtcccg gtagcttctc
     4021 ggtgaagaac gcactgttgt cggcgaggaa atgctgcaaa gtcctcaatc catgcgccga
     4081 aacggtattc ccgcagagaa agtgggagta aaggcctgct ggaatacagt ttcggcgaaa
     4141 tgagaagcta ggcgccaact tcgacgatgc cgtcggcggt gatgcgcgtg gcgtacaccg
     4201 ggagcgaaac ctccgggtcg tcgaggcagc tgccgtcgtc aagagcgaac gcctgcttct
     4261 tgatcggtgt ctgcacggtc gcacgcccgc cgcggtcgcc gacgatgccg cgcgacatca
     4321 ccgccgctcc cgagaacgga tcgatgttgc ccaccgcgtg cagtgacccg tcgtcgagac
     4381 ggaacagcgc ggcctgggca ccgtcgggca gcagcactcc cactccacgg ttggggatca
     4441 ggaagtcgta cgcgcaggcg gtcgtccaga ccgcaacgtc gttgagcaga gtcatatttg
     4501 gctaaccccg tactttcggc ataccgatcg gaacaatacg gcccgactcc tcggtgaact
     4561 tgaccgttgg atccacgacg tctggagcgt tgacgaacga gacgaatcgc gacagcttgt
     4621 tcgggtcctc cagcacgccc ttccattcgc acgcgtagcc ggccacgtgg cgttccatgg
     4681 cttcttcgaa ctccgcggcc agaccgagcg agtcgttgca gacgacgtcg cgcaggtgat
     4741 ccagcccgcc ctccatcgcc tccagccacg gcgcggtgcg ctgcagccga tcagcggtgc
     4801 ggatgtagaa catcaggaac cggtcgatgt agcgcaccag cgtctcgtcg tccaggtcgc
     4861 tcgccaacag ctgcgcgtgc cgcggcgaca tgccgccgtt gccgcagacg tagagattcc
     4921 agccctgctc ggtcgcgatg acacccacgt ccttgctctg cgcttccgcg cattcccgcg
     4981 cgcatcccga caccgccatc ttgatcttgt gcggtgcccg cagaccccgg tagcgcttct
     5041 cgatctcgac ggccatgttg accgaatcct gctggccgta gcggcaccac gtgctgccca
     5101 cacagctctt caccgtccgc agcgccttgc cgtacgcgtg gccggactcc atgccgccgt
     5161 cgaccaggcg gcgccagatc tcgggaagct gatcaacccg tgcgccgaac atgtcgatgc
     5221 gctgcccgcc ggtgatcttg gtgtacaggt tgaagtcgcg cgcgatctcg ccaatcagga
     5281 tcagctgctc gggggtgatt tcaccgcccg gcgatcgcgg caccaccgag tagctgccgt
     5341 tcttctggat gttggccagg aagtggtcgt tggagtcctg cagcgaggcc tgctcgccat
     5401 cgaggatgtg ctcggagctc gtcgacgcca ggatcgacgc caccaccggt ttgcagatat
     5461 cgcaaccctt tccggtgccg aagcgttcga tcagcgcggt gaaggtgcgg atcgaggtag
     5521 cggcgacgat cgagaacagc tcggcccgcg actggctgaa gtgttcacac agcgccttgg
     5581 actgctcgac accctcggcc tccagcagct gcttgagtag cggcacacac gagccgcacg
     5641 aggtgcccgc cttggtgcag ctcttgagcg acggcacatc gctgcagccg ccggcgatcg
     5701 cgtcgcacag gtcacccttg gtgacgttgt tgcaggagca gatctgtgcg gccgctggca
     5761 acgcgccgac gcccaaagtg cttgagccac cgtcagatcc ggccggggca atcagcgcca
     5821 tcgggtcgcc gggcaggggc tcgccgacca tcggccgcag cacgccgtac gaggacgcgt
     5881 cgccgacgag gatgccaccc aggagcgtct tggcatcatc ggacagcacc agcttggcgt
     5941 aggtctggtt caccgggtcg ttgacgacga cgtcgaggca gctcggtgtg ctgcccatcg
     6001 cgtcgccgaa gctggcgacg tcgacgccga gaagcttgag cttggtcgac atgtcggcct
     6061 cggggaactc cgcggcgccg ccgagcagcc ggtcggcgac gacttcggcg ctggtgtacc
     6121 cgggtccgac gaggccgtag cagcggccct cgatcgccgc cacctcgccg atcgcgtaga
     6181 tgttgggatc tcgtgtggca caagacagat cggtgagcac accgccgcgc tcggcaacat
     6241 cgatgtccgc cgcccgggcc aactcgtcgc gcggtcgcac acccgccgcg aacaccacga
     6301 cgccggcgtc gatggccgtt ccgtcgctca gcgtcacccg caccgcgtcg tgatcggcgg
     6361 accgccggac gggccggttg cgttgcgccg gctcaatcgc ttgggtgccg acgccgacat
     6421 gcacggtgat gccgagctcg ccgatcatcc ggccgagcag cgcgccgccc gcctcgtcca
     6481 gctgctgggc catcaggcgg ggcgccatct ccacgacgtg ggtctgaagg ccgaactggc
     6541 gcaacgcatt agcggcctcc aggcccagca gtccgccgcc gatcaccacg ccaaccgggg
     6601 cgggcccgcc gacagcacgc atcgcgtcgg cacggatcgc atcgaggtcg tcgagggtgc
     6661 ggtacacgtg gcacggcgga aggtcgtggc cgggtaccgg cggcacaaac gcatacgagc
     6721 cggtggccag caccagcgcg tcgtagtgca cacggtcgcc gttggctgtc accaccgcct
     6781 tggccgagcg gtcgatctcg gcgacccggc tgccgagtcg cagttcgacc agttcgtcac
     6841 cggggtagtc gttgccgggc agggcgagca ggccgcggtc ccagtgctcg gtgtagccgg
     6901 tgagcccgac gcggtcgtat gcggcgtcgg cttcctcggc aagcacggtc acccgccagg
     6961 cgccgtcgcc atcgcgcgac cgcagtgcct caacgaaccg gtgaccgacc atgccgtggc
     7021 cgacgaccac gacgcttgat tttgaccgca tgccgcagag gctaagcagc ggatattgcc
     7081 gcaatgtcgc ctcatgtgaa gcgcccatca cggagtcctc accggcgccg cccgcagggt
     7141 gtgagccggt tcgccgacgg cgaacgcgct gctggaacat gagcgtgggt gactcaagtt
     7201 atatagacag cagaccggcg tggtgccggt cacgacagag caaggagata ccaccgatga
     7261 gcaacgtgac gctatgggcc cggcccgcgt gggagatcga ccgttgggta aagcagggcg
     7321 tcttcaacgg cttcagcccg gccgccgagg tcgccaagga cggcgacgac gcggtggtcc
     7381 gtgttgagct gcccggcgtc gacgtcgaga aggatgtcaa cgtcgaggtc gaccggggcc
     7441 agctcgtgat tcgtggtgag cgccgcgacg aacgctcgga atccagcgac gggcgtacgc
     7501 tcagcgaggt ccgttacggt tcgttccggc ggacgttcaa gctgcccgcc cacgtcacca
     7561 gcgacgcgat ctcggcgtcc tatgacgccg gtgtgctgac cgtacgggtc gccggcgccc
     7621 acaagggtgc cgaagcgcag cgcatcgcga tcgagaacaa gtaacactca cgaccaaatc
     7681 cggtggagcc caaaaggttc caccggattt gtcattaggt caccgatacc aggtactcgc
     7741 cgcggtagcg catggtcccg tcgtctgcct cgttcacatc taccgcccaa tccaggtagg
     7801 tctcgcgaac cagctcgtcg ccgagctcgg cgcgcatggc gatcagcggc ggaaagctgg
     7861 tcagcatgaa atcgacgtag ctctcgggcg agggctcgtc gaactcgacg ttgtgccgct
     7921 cgaactcaac ggagtcaccg agcagttcgc gcacgtgctc ttcggtaccc cacagcaccg
     7981 gcggttggaa gcctgccggc ggcgcgggcg aaattgcgcc gagccgcttg aacatctcgc
     8041 cgatggcgcc caccggtgtc cagcacgcga tcgcgatgcg gccatccggc ctgcatacgc
     8101 gtcgcaactc atccgcggtg cgcttgtgat cgggcgcgaa catgtgcccg atcgctgaga
     8161 tgactcggtc gaaactgcca tcgtcgaatg gaagcgactg ggcatcccct tccagccagt
     8221 cgacatccac cttggcctcg gcgccccgtt ggcgtgcgat cgcaatcagg cccggggaaa
     8281 agtccaggcc ggtgacccgc gcgcccagcc tggccgccgg gatggacgcg ttgccggttc
     8341 cgcacgccac gtccagtaca tccatccccg gctcgatgtt ggcccgcgga accaggacgt
     8401 ccgcgatcga ggtgactttc tccgccactg cccggtagtc gccggtcccc cacatgtcat
     8461 cggtcatggc cgtaagcctt cgccggtcac cgcagcaggt agaggatttc ccgtattcgt
     8521 cgccaggcta attctcttcg accccgaacg cgtctcccgt gtccaccccg atgacccgca
     8581 gcaggttggc caccttgcgg tccagactca agcccacctc agtgacctca ctgatgccga
     8641 ggctcgcgaa cgccgagctg ggttgatcca tcgaaaacac cgcgttgccg tcagggtcgc
     8701 tgtacacaag cagccgcagg ggcgcgtaca gcagcgccct ggggtcatgg cgaaacatcg
     8761 tttcggcgat gacgtggttg ccgaccagat actcgaccgc ctttacgtca tgaccggcga
     8821 cgccgagcag cggagtggca tcgatcttcg cgtagaccat gagttcgttg ggcgcgttgt
     8881 cggccacctt ggcgcgcacc tcgtcccagg taccaccgga gtcgacgatc cgttgcatgg
     8941 gaacggggtc gaaggacggc gccgcgtcct cgaaagccct tagaaactcg tcgaactcga
     9001 cgccagtgag cacgtcgacc ctcgtcatcg aatggtcgtg cctgtgggcc tgggtcatgg
     9061 cgttccccct agatgtgacc ggcgttggtt ggagtcgtcg tgaggtatgg gttggtggtc
     9121 gtggtcgagg ttgtcgtttc gtcgtcagtc ggcccgtcgt cgggtgccgt cgactcgggt
     9181 ggccgagtca gagccgggtt gtcctggctg gtttggacac tcggcaccgc gtagctcgtg
     9241 ctcgacgtcg actccgtcgt gtacgtcgac gaggtcgccg aggacgtggg gaactcgact
     9301 gggtcgggcg gtccagaaga gttgtccgcg gtgcggatca ccgcccacac cagcagtgcc
     9361 agcagcagca gccccgcgac tccggcggcc acgattaccg gttgctgatc gtgccagctg
     9421 ggctcgtcgt cgtcagggct cacggccgga atgctaggcc cagcgggcca acatctcctt
     9481 ggagtacgcg gacagcgcga actgccagaa cgggccgaag ctgacccggc ccaccccgag
     9541 cggaccgaac gacgcagggt cgtcctggtc gggcagcgcg atcgcgttga tcggcagcgg
     9601 gagctcggcg gccaagcgcc gcaacgtatc cgggtcgtgg cggcccacgg ggtacagcga
     9661 gtcggcaccc gcggtcgcgg cttccttcag ccgcgcgatc gcccggtcga cgcggtcggc
     9721 ttcgtcaccg tcctgacgaa ggaacaggtc cgtgcgggca ttgatcacca cgtgcacacc
     9781 cgtagcgtcg gccgccttcc gcagggcgcc gacgagctcg gcatgctcgc tggacgaccg
     9841 cagccgtcca ccttcgctgt gcaccgtgtc ctcgatattg aggccgacgg cgccggcgtc
     9901 cagcaggccc gtgatcaggc gggtgggcga ttggccgtaa cccgattcga tgtcgactga
     9961 caccggcacg tcgaccgccg ccgtgatctg cttgacgcgg gtgaccacct cgtcgaacgt
    10021 catgccctcc tgatctgcct tgcccaccga atcggccatc ggatggctac cgacggtcag
    10081 cgcgacgaat cccgcgtcga ccgccagccg cgccgaccac gcgtcccaaa ccgtcggcaa
    10141 taccaccgga ttccccggct ggtgcagcgc cagcaacgcc tcggcgcgct gcttcaaaac
    10201 ctggtcagac atcaagctac ctccgcctct ccgacgtgat ctgtctcacg ctgaactcca
    10261 tgttgtggct agcatcgggt gtcgtactgt gatcccagac acccttcagc ccaaggaggt
    10321 caattgtcta ccaagactga acttgccgag ctgcacgagc taatcggcag catgcggcgg
    10381 tgcgtaacag cgttggcatc taagtatggc gacagccccg ccacccgtcg catcgtcaac
    10441 gacgccgagc gcattctcaa cgacattgac cggctggata tcgacgctga ggaactggag
    10501 ctcggcagtg gtgtgacccg ccatcacggt ggcgagaaga tcgcgatccc ggacaccccg
    10561 cacgagaccg agttctgggg cgacgaggac gacaggggcg tcgccggcta ggccacctcc
    10621 gccgcctcgg cacctaacca ccgaaaggga aaagtgagcg cacccaccgc cgaccgcaag
    10681 gcgaccggcg ttttctcgcc gagccgtgcc cagattccgc agcgcactct gcgcaccgac
    10741 cggtggtggc tgccgacgct gtggagcaac ctcggtttcg ccgcattcct gctctacggg
    10801 ctggttcgag cgtcctggca gagcgcctat tgggttgagg actaccacta cctgacgccc
    10861 ttttattcac cgtgtatcag cgcatcctgc gcgccggggt ccagccacct cggcgtctgg
    10921 cttcccgagt ttccgccgct ggttccgttg gcgatcatca cgctgccgtt cttgctgctt
    10981 ttccggctga cgtgctacta ctaccgcaag gcgtactacc gctcggtgtg gcagtcgcca
    11041 ccgggatgcg ctgtggcaga accgcattcg acctacaccg gtgagacccg gctgccgctg
    11101 atcattcaga acactcatcg gtacttcttc tacgccgccg ttctcatttc agtgctcaac
    11161 acctacgacg cgatcgtcgc gttccactcg ccgtccggct ttggattcgg cttgggcaac
    11221 atcgtcctga cgggcaacgt cattctgttg tggatctaca cgctgtcctg ccactcctgc
    11281 aggcacatcg tcggcggcaa gctcaagcac ttctcgaaac atcctgtgcg gtattggttc
    11341 tggaatcagg tgagcctgct caacaaccgg cacatgatgt gggcgtggat caccctgggc
    11401 actctggtgc tgaccgactt ctacatcatg ctggtagcca gcgggactat ctctgatctc
    11461 agattcattg gctgagaaca ggtttcattg catttacagc gaatagtgag ggtttatggc
    11521 tgaggtcgaa cggcatcaat acgacgtcgt cgtgatcggt gccggcggcg cgggtttgcg
    11581 tgcggtcatc gaggcgcgtg aacgcgggtt gaaggtcgcg gtggtctgta agtcgttgtt
    11641 cggcaaggcc cacacggtga tggccgaagg cggctgcgcg gcggcgatgg gcaatgccaa
    11701 cccgaaggac aactggcagg tccatttcaa agacaccatg cgcggcggga agttcctgaa
    11761 caactggcgg atggccgagc tgcacgccaa ggaggcgccc gaccgcgtct gggagctcga
    11821 gacctacggc gcgttgttcg accgcaccaa agacggtcgc atcagccagc gcaacttcgg
    11881 cggccacacc tacccgcggc tcgcgcacgt cggtgaccgc accggcctgg agatcatccg
    11941 caccctgcag cagaagatcg tctcgctgca gcaggaggac aaggccgagt tcggcgacta
    12001 cgaggcgcgg atcaaggtct tccacgaatg cacgatcacc gacctgatca aagacggcga
    12061 acgcatcgca ggcgcattcg gctattggcg cgaaaccggc cgcttcatcc tgttcgaggc
    12121 gcccgcggtc gtcctggcca ccggcggcat cggcaagtcc tacaaggtga cgtcgaactc
    12181 gtgggagtac accggtgatg ggcacgcgct cgcgctgcgg gcaggcgcga cgctgatcaa
    12241 catggagttc gtccagttcc acccgaccgg catggtgtgg ccgcccagcg tcaaaggcat
    12301 cttggtcacc gagggtgtgc gcggcgacgg cggcgtgctg aaaaattcgg acggcaagcg
    12361 gttcatgttc gactacatcc cgccggtctt caagggtcag tacgccgagt cgatcgaaga
    12421 ggccgaccag tggctcaaag acaacgactc cgcgcgccgc acgcctgatc tgttgccccg
    12481 cgacgaggtg gcccgcgcca tcaactccga agtgaagggg gatcgcggca cgcctcatgg
    12541 cggcgtctac ctggacatcg cgtcgcggct gcccgccgag gagatcaagc ggcggctgcc
    12601 gtccatgtat caccagttca tggagctggc ggaggtcgac atcacgaagg agccgatgga
    12661 agtcgggccg acgtgccact acgtcatggg gggtgtcgag gtcgaccccg acactggcgc
    12721 cgccaagacg cccggcctgt tcgccgcggg cgagtgctcg ggcggcatgc acggttccaa
    12781 ccggcttggc gggaattcgt tgtcagatct actggtgttc ggccgccgcg ccgggcttgg
    12841 tgcctccgac tacgtccggg cgctgtcgga gcggccccaa gtgtccgagg acgccgtggc
    12901 cgaggcagcc aagctggcgc tcgcgccctt cgacggcccg accaacggtg acagcgcgga
    12961 aaacccgtac acgctgcagc tcgacctgca ggacacaatg aacacgctgg tcggcatcat
    13021 ccgtaaggcc gacgaggtgt cggaggcctt ggacaagctg aacgagctgc gcgagcggtt
    13081 caagcgtgtg caggtggagg gccaccgcca gttcaacccg ggctggcatc tggcgatcga
    13141 cctgcgcaac atgctgctgg tcagtgagtg cgtggccaag gcggcgctgg agcgcaccga
    13201 gagccgcggc ggccacaccc gcgacgacca cccgtcgatg gacccggaat ggcgcaagac
    13261 actgctggtg tgccgcgcca tcgacgatgg agccgacgtg tccgttgttc ccgacgtcga
    13321 catcacgcgc gaggaccagg tgccgatgcg tgaggacctg ctcgagctgg tcgacatcga
    13381 agagctggag aagtacttca cccctgaaga actcgccgac cacccggcaa ggagaagctg
    13441 atggcttatc aagcgaagat gcgggtctgg cgcggcgacg acgacggcgg ggcgctgcag
    13501 gactacgccg tcgaggtcaa cgagggcgag gtggtgctcg acatcatcca ccgcctgcag
    13561 cagacccaga cgcccgatct ggcggtccgg tggaactgca aggcgggcaa gtgcggttcc
    13621 tgctcggcgg agatcaacgg ccggccgaag ctgatgtgca tgacccggat gtcgaccttc
    13681 gacccgaccg agaccatcac ggtgacgccc ctgcggacct tcccggtgat tcgcgacctg
    13741 gtcaccgacg tctcgttcaa ctacgaaaag gccagggaaa tcccgtcatt cacgccgccg
    13801 aaggatctgc agccgggcga gtaccggatg atgcaggagg acgtgaaccg gtcacaggag
    13861 ttccgcaagt gcatcgagtg cttcctgtgc cagaacacct gccacgtggt gcgtgatcac
    13921 gaggagaaca agcaagcatt cgccgggccg cgctacctga tgcgccaaac agaactcgac
    13981 atgcatccgc tcgacacgct tgcgcgtcgt cccgagcagg cgcaggaaga aaacgggctg
    14041 ggcttctgca acatcaccaa atgctgcacc gaggtgtgcc ccgagcacat caagatcacc
    14101 gacaacgcac tgatcccgct gaaagagcgc gcggtcgaca agaaatacga cccggtggtg
    14161 tggctcggaa acaagctgtt cagacggtga tgagcgcttg cgcgaagaac agaagacgaa
    14221 gttgagaagg aggctctcgc gggtagtctt cgtacagcgg ccaatcaacg acgaaaggca
    14281 gcagtatgac tatgcgagag cctcatagca tcaacgacat tcgcaccgcg attcgcgagc
    14341 tgaccacgcg ggccgaactg gcccgtaaag aaggccggcc agccgacgct gaggaactcg
    14401 agcagcgtat tcagagctat cgcgacgagc tcgcagctcg tccgtagtca ggcaccaagc
    14461 cgccgaaatg tgctgcggtg gaacacaatc ggcgggacat cggcatgcac ggtgatatcg
    14521 ctcacccgta gaatcactat cgtgtgatcc ccggcgggca ccagctgttc gatggcgctt
    14581 tccagccaca cactcgtgcc cttgacgaac acggcaccgg actcccggga caccgtctcg
    14641 agacccgcga accggtcgcc ggtcttggcc gccaatgtgc gagcggcgtc gtcatgggcc
    14701 tcgccgagca cgctgatccc caggtagggc aggcccttca acttcggcca tgtctcagag
    14761 atgttctgta cgcagaacga caccagcggc ggatccagcg acacaggaac aaaggtgctg
    14821 gccgccagcc ccaccagggt cccgtccacc tcggcggcga tcgcgatgac gccggagggg
    14881 aaatgcccga acgcttcccg cagcgtggtg ggggtcagat cggtggaact catcaactcc
    14941 atcttcacac gtatgcgcgg acggcggcga tggtcgcaat ccccatgaat ccaacgccta
    15001 gtcggcccag cgcgatgctt ccacctcggg cggcagtgtc gactgcatcg ggacgtcgag
    15061 cccgtcgtag atgcccggct tctgctccgc cagccagtga agggcaccga gcaagcggtt
    15121 cgctgcggtg gtgttgccgc cgtcagcgcg cgttccgccc gggacgtcag cgcggctggt
    15181 gagtgtcaac tgcgggtcgc cgtcgatgat cacccggtgg tcaccgactc cttcatcggg
    15241 atacggccag tccggcgcgc acgaggcgtg aatccgagtg atgtggtcga tgatgacgcg
    15301 ctcccgcccc ttcgacttgc caatcacctt gagccagaac gcgccttggg tgctcttctc
    15361 gaaccgaccc atcacggtgt cgacggactc gtcgagcggc aaccgctcga cttcctcggt
    15421 gatctcgtcg atctccaaac ccaggccccg gccgatcaac cggatgttgc cgccccacac
    15481 catggtcggg atggacggca gcagcatcat cggcactggg tcttctcggc ccatggccga
    15541 tccgaacccg cacgacacct tcaccgcata cggctggttg taggtcgagt agtcgaagat
    15601 ctcctggcag ttgatggtcc ggatacgggt acatagcccc gcggcgatga cggccagtgc
    15661 gtcattgccc cagcccggat ccacgccact gacgagcagg gtcgccccgc cttcctcggc
    15721 ggccgcggtc agccgctgca cccattccgg aggtgcggac cgcgggtcgt agagcgagta
    15781 cagcgacggc gtcaccacat gcttgcctgc gcgcaggcac cgcacgatct cgtcgaccgc
    15841 ctcgtcgggc cggatgtcgc ccgacgacat gtaggccacg gcgtcgcagc cggctagggc
    15901 ggcatcgacg tcggtggtag ccaggacgcc cgtttccgag tcgagccgcg cgaatgtcgc
    15961 cgcgtcccta ccttccttgt ccggcgacga cgtgatgacg ccggacagct gcaacccggg
    16021 aaacgccgta gcggaccgga tcgctgtcgc ccccatattc cctgttcccc acaccgcgac
    16081 acgaagcacc cgaccaccct atccgtggcg tcgtgatgaa cctcacagag gggcgaagat
    16141 gcagctcaga cacctgtcaa ctgcggtttt ggcggcccac ccaaccactg ggttacttag
    16201 ctggcggaaa ttgctcacag cggctttgtg ttgaagttac tgaacggtat agttcgggcc
    16261 agttactggt gggtaactta gacaacgagt acccaaccgc aagtggccaa ctcgacgagg
    16321 aggaaacgtg agccactaca agagcaacgt ccgcgaccag gtattcaacc tgttcgaggt
    16381 gctcggcgtg gacaagacgc tcggccaggg cgactacgcc gatctggacg tcgacaccgc
    16441 caaagagatg ctggccgaga tggcccggct ggcggagggc ccggtcgcgg aatctttcgt
    16501 cgaaggtgac cgcaatcccc cgacgtttga ccccaacacg cattcggtga cgctgcccga
    16561 cgcgttcaag aagtcggtca acgccgtgct cgaggccggc tgggaccgag tgggcctgga
    16621 tgaggccctc ggcggcaccc cgacccccaa ggcgctgatg tgggcgctca acgagcacat
    16681 cctcggcgcc aaccccgccg tgtggatgta cgccggcggt gccgggttcg cccagatcgt
    16741 gtaccacctc ggtaccgaag agcagaagaa gtgggccgtc atcgccgctg agcgcgggtg
    16801 gggctccacc atggtgctca ccgagcccga cgcgggctcc gatgtcggcg ccggacgcac
    16861 caaggccgtc aaacaggacg acggctcgtg gcacatcgac ggcgtgaagc gcttcatcac
    16921 ctcgggtgac tccggcgacc tgttcgagaa catcttccat ctggtgctgg cccgccccga
    16981 gggcgcaggc ccgggcacca agggtctgtc gctgttcttc gtgccgaagt tcctcttcga
    17041 cttcgagacc ggcgagatcg gcgagcgcaa cggtgtgttc gtcaccaacg tcgagcacaa
    17101 gatgggcctc aaggtctcgg ccacctgtga gctgtcgctc ggccagcacg gcgtgcccgc
    17161 caagggctgg ctggtcggcg aggtgcacga tggcattgcg cagatgttcg acgtcatcga
    17221 gcaggctcga atgatggtgg gcaccaaggc cattgccacc ctgtcgacgg gttacctcaa
    17281 tgccctggag tacgccaagg agcgtgtgca gggtgcggac atgacccaga tgaccgacaa
    17341 gaccgcgccg cgcgtgacca tcacgcacca cccggacgta cgtcggtccc tgatgaccca
    17401 gaaggcctac gccgagggtc tgcgtgcgct gtacctgttc accgccacct accaggacac
    17461 cgcggtggcc aaggccgtgc acgatgttga cgccgagctg gcggtcaagg tcaacgacct
    17521 gatgctgccc atcgtcaagg gtgtgggttc ggagcaggcc tacgcgaagc tgaccgagag
    17581 cctgcagacc ttcggtggct ccggcttcct gcaggactac ccgatcgagc agtacatccg
    17641 tgacgccaag atcgactcgc tctacgaggg caccacggcc atccaggcgc aggacttctt
    17701 cttccgcaag atcatccgcg acaagggtgc ggcgctggcg tacgtggccg gccagatcga
    17761 gcagttcgtc aagagcgaat ccggcaatgg ccggctgaag gccgagcggg ctctgctggc
    17821 gaccgcgctg gaggacgtgc agggcatggc cgccacgctc accggctacc tgatggcggc
    17881 gcaggagaac ccggccgagc tctacaaggt tggcctgggt tcggtgcgct tcctgatgag
    17941 cgtcggtgac ctggtcatcg gctggctgct gggccgccag gctgcggtgg ccgtcgacgc
    18001 gctcgacaag ggtgccagcg gcgacgaccg cgccttctac gagggcaagg tcgcggtggc
    18061 gtcgttcttc gcgaagaact tcctgccgct gctgaccagc acccgcaccg tgatcgagaa
    18121 cctcgacaac gaggtgatgg aactcgacga ggcggccttc tagaccgctt cgtacgcgac
    18181 aggccccgga ctcttcccgg ggccttttgc gttggtcctg cggcaaactg gacgaatgac
    18241 caatgtcgct cgcgtcgttc tgtttcttgc gctggtggca acaaccgtgt tgacattcgt
    18301 ccttggcgtc acggatccga ccggcacgcc gtcctatccc acgaagtttc tcgtctggaa
    18361 tctggttctc gcgtggatcc cggtattttt cgcggcctgc tttgcggcgg tgcgccttcg
    18421 cctttggctg ttgccgttgg gtttggcctg gctggcgttc ctgccgaatt cgccgtatct
    18481 ggtgaccgat ctcgtgcatc tcgaggaagg cgtggacttc tggcgtcacg tcttgcagta
    18541 cgggttcgcg gcctggacgg gcatcctcct cggcgtggtg tcgttgcgac tggtgcatga
    18601 tcgcatcgag cgggatttcg gcgcgttcgc cggttgggtc gcggtcatcg tttcgatccc
    18661 actatgtgcg atcggcatcg tgatcgggcg gttccagcgt tggaactcct gggacttggt
    18721 gagacggccc agagaagtgc tagggaccac actggagtgg atgcgctcgc cgttcgcaga
    18781 caccacgtcg atcggggtgg cgctggcggt ggcagcgttc ttcgggctgg catacgtgac
    18841 catttggtcg ctcggggccg cctacggtcg gaccgaactc aatatgaggt aaacacctca
    18901 tattgccggg atgggtcgac cgagcaaact aaccggcgaa cgagttctcc aagaatcgct
    18961 tgagaatccg ccgagtcctg ggccgcagcc tttctgcgag ccggtcgaca ccggcaacca
    19021 gccaaaccga tttgtcagaa aggccatctc catgaccaca ctgtcgccac ccaataagcg
    19081 gttgagcaaa cgcgccaaga tggtgatagt cgttgccgcg gctttccttg tggccgtcgc
    19141 cgcagtcgcg agcatcatcg cggtcgttgg ccatcagacg cttgagaaga agaccgtgtc
    19201 gtattcgtct caggtcgccc tcccgttcac caacctcaag attccggtcg gtatcgcagt
    19261 tgacgccgcg ggcagcgcgt atgtcgccga ctactacgac agcatcgtgt ggaagctgcc
    19321 accgggagcg gaggtcccaa tcgcgttgcc gttcaatgac atcaagcacc cgactggtgt
    19381 ggcactggat acctccggca acctctacgt caccgatggg gacaacaacc gtgtcctgaa
    19441 gctggcgccg ggagcaagtg acgcgaccgt gatgccgttc accggactca agaaccccac
    19501 gggcgtggcg gtcgacaccg caggtaacct ctatgtcgcg gacttcaaca acagccgcgt
    19561 gctgaaactg tcggcgggcg cgaatgcccc agaagtgttg cctaccaagg gtctcaagta
    19621 ccctgaaggt ctcgcgatcg acaccgcagg caacctctac atcgctgaca aaggcaatcg
    19681 acgggtcctc aaactcacgg cgggagccaa ggcaccagaa gtgctgccgt tcaccgggct
    19741 caaggacgcc gatggcgtcg ccgtcgacgg caagggcaac gtctacgtcg cagaccaagc
    19801 cagtcgaaag tggtggatgc tggccgcggg ttctcattcc cagacggaaa tgccttccga
    19861 gtacataggc ggtccgtttg gtgtcgcagt ggacgccggc ggcaccctgt acgtcacgga
    19921 cgccggcaac ggcacggtgc acaagctggc ggccggggcc aacaaactga cggtgctgcc
    19981 tttcaacaac gtctctaccc cctggggcgc agcggtcgat gcttcgggga acctgtacat
    20041 cgccgaaagc gaacatgtgt tgaagcttgc cgcgggtgcg agcgcccccg ccgtattgcc
    20101 gctccccggc gtccgggcac ccgacgcggt ggcagtggat accgcgggca atctctacgt
    20161 cgtcgaaccg gacagccaga ggtacatcga gaacaacaac cccaaggtgt ggaagctcgc
    20221 cgccggggca accgagccaa ccctgctgcc gttccccggc ctcaaggcac cctcagcggt
    20281 agcggtcgac accgcgggca acctctacgt cactgacatg tacaaaaacc gggtatggaa
    20341 gctggcggag ggagcgaaca acccaattgc gttgccgttc aaagacctcg agaaccccgc
    20401 tggtgtggcg gtcgataccg cgggcaacgt ctacgtcgcc gatcgtgata ccaagcacgt
    20461 ttatgaactg gcggcggggg cgaatagcgc gaccgtgatg ccattgcctg gcctcgaacg
    20521 tctggatgcg atcgcggtgg acagcgccgg caacctctac atcaccgacg cccatgatcc
    20581 ctgcacccat tctggcccat gcaccttcat cgacgagact ggtcgcgtcg aggagggccg
    20641 ggtgttgaag ttcgccacca attcgaccgt gccgacagtg ttgccgttcg acggcctgga
    20701 ttactccagg ggtatcgcgg ttgatgccgc ggagaacgtc tatgtcgtcg atggcagcga
    20761 ccgggtgctg aagctgcctg tgatgcaggg cgaggacgaa tagccgtcag gagttgaggt
    20821 aggtcaccac cgccagcacg cggcggtgat gcgcctgcga cggcggcagg tccagtttgg
    20881 tgaagatcgc cgacacatgc ttctcgaccg cccgctcagt gatcgaaaga tgctgcgcca
    20941 gcgcgctgtt ggaatggccc tcggccatca gctcaagcac ttcgcgttcg cgtgccgtca
    21001 accgatccag aacggtggct tgccgccggc tgccgatcag ttgacgcacc acctccgggt
    21061 cgagcgcggt gccgcccgac gcaacgcggc gcaaggccgc gtcgaactcg gcgatgtcgg
    21121 tgacccggtc cttgagcaga tagcccacac cctccggatt gcccgccaat agttcagtgg
    21181 cgtaacgggt ttcgacccat tgcgagaaca gcaacacacc gacatcggga tgcgagcggc
    21241 gtagcgccac cgcggccacc aagccttcgt cggtgaacgt cggcggcatg cgaatgtcga
    21301 cgacggcgac gtccggtgtg aacgcgttca ccgcttcgat caattcgtcg gcggtgccca
    21361 ccttggccac cacctcgtgg ccccgttcga gtagcaactg cacgagcccg tcgcgcagga
    21421 tcgcgctgtc ttcggcgatg acgagcctgg tcactgctcg gtccccggcg gaacaacgac
    21481 cgtgacaacc gtggggccgc cgggcgggct gtcgatctcc aaccgaccgt cgagcatcgc
    21541 aagccgatcg cgcagccccg tcagccccga gccgttgccg acctgtgcgc caccggagcc
    21601 gttgtcctcc acgatgattc gcaactcatc cgcgttgccg tggatccgga ccgaggcgtg
    21661 gctggcaccc gaatgccgtg acacgttggc cagcagttcg gcgacacaga agtaggccat
    21721 ggtttccacg ccgatcgacg gccgggtcgc gacgtcgacc gccacctcga ccgggatggg
    21781 gttgcgtgcc gccaaggtct gaaccgcttc gccgaggccg agatccagtg cgggcgggcg
    21841 gatcccgcgt atcacatcgc gcagctcggt gagcgtgtcc ttggtattgg ccagcgcgtc
    21901 ggagaccagt tcacggccac gcgtcacgtc ggacgctgcg aagtgctcct cggcgcgagc
    21961 caacgccatc gcgaccgtga tcagccgggc ctgtgtcccg tcgtgcagat cacgctcgac
    22021 gcggcgcagg gtggcggccg ggtcgtcgac cgcatccgcg cgggcccgtt ccagctcgcc
    22081 gaccctccgc tcccgttcgg tcggaccgag cagggcgccg gccacccaca cgtgggcgcg
    22141 gccgagcccc agcatcagcc agcccaggcc gtacagcgcg acgacgccgc cggcggacat
    22201 caacagatac gccggcagtg agtcgaccgg ttcgccaagc gtgacgaacg gctcgcccag
    22261 cgccgcccac acgacaggcg agaccaccaa cgctccggac attgccacac cgaccaagac
    22321 gaagtaaccg atcggtgtca tcagcaccgc gtgcagcgca aggaatccga ggctgcgcca
    22381 gccgactccg tcggtcagcg cggccgcaac cgtgtgcagc cggcccgcgg gtcgaacgaa
    22441 tggtggcggc gcgtcgatcg tcgtacccag cagccgcgcc agtgaccggt acaggcggtt
    22501 ccatgcgcgg ccgctcatca ccaccagagc cagcagcgga atgccgacga gcgtggccat
    22561 cagcagcccg gaggcaaggc ccgcgaagaa gatgaacgcg acgccgacga ccgcaaggac
    22621 gctgaccagc acgaagtaca ggtaggcccg ccacgccggg gcacgcagcg gaaccagcag
    22681 cgttcgggtc caatccgatt gggtcgcaac ggcagtcgtc atcacgtcag cgatcgtcgc
    22741 gccgcgggcc cgccctccat ggtggtgtcc cgcgaatgtg tggtggggct agccacaccg
    22801 gccatgcaga caaaaagggc gggttcggga ggacacttga gggcgtctca ttagttctcg
    22861 cgcgtccggc tttcacctct atgccggcac ttcggatcag tcactcccga acccgtcgtg
    22921 cgctcgatcg tacgcctgtg attgcggcca caccagagga aatttgggcg ctatttgtcc
    22981 cttgttcgca gtagtgcgcg ggtgcgttca cggccgtcgg cagacgggtc gaaggtgacg
    23041 gcggcgaact cgtcgacgcc cgctgcggcc agctcgtcga gacggtcgga caccgttttc
    23101 tcgtcaccga tgatcgcggc atcctccggt cccgcgtatc cctcacggtc gagcatcgcg
    23161 cggt
//
LOCUS       DASRJA010000385         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_946680,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000385 DASRJA010000000
VERSION     DASRJA010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946680"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1262)
                     /gene="proB"
                     /locus_tag="VFC01_17110"
     CDS             complement(159..1262)
                     /gene="proB"
                     /locus_tag="VFC01_17110"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HZN81386.1"
                     /translation="MTSQHREAVRTARSVVIKVGTTALTTPSGVFDASRLAVLADAIE
                     GRMKAGSDVVIVSSGSIAAGIEPLRLSKRPTDLATKQAAASVGQVALVNSWSTAFAHY
                     HRTVGQVLLTAHDISRRVSHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRL
                     SALVAHLVGADALVLLSDIDGLYDSDPRKGNARFIPEVAGPADLDGVVAGQGSHLGTG
                     GMASKLSSALLAADAGVPVLLAAASDAATALADASVGTVFAPRAERMSARRFWVRYAA
                     ESAGALTLDDGAVRAVVRQRRSLLPAGITGLSGRFYGGDVVELRTQDATMVARGVVAY
                     DAAELATMLGQSTSDLPAEMRRPAVHADDLVAV"
     gene            complement(1259..2698)
                     /gene="obgE"
                     /locus_tag="VFC01_17115"
     CDS             complement(1259..2698)
                     /gene="obgE"
                     /locus_tag="VFC01_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZN81387.1"
                     /translation="MPRFVDRVVIHARAGNGGNGCASVHREKFKPLGGPDGGNGGRGG
                     SVVLIVDPQVHTLLDFHFHPHIVAPSGKQGAGSNRDGAAGADLEVKVPDGTVVLDDNG
                     RLLADLVGAGTRFEAAAGGRGGLGNAALASRARKAPGFALLGEKGQVRDLTLELKTVA
                     DVGLVGFPSAGKSSLVSAISAAKPKIADYPFTTLAPNLGVVSAGDHTFTVADVPGLIP
                     GASDGRGLGLDFLRHIERCAVLVHVVDCATLEPGRDPISDIEALEAELAAYTPTLQGD
                     STLGDLAERPRAVVLNKIDVPEARELADFVREEIVRRFGWPVYEVSTVTHEGLRPLTF
                     ALWDMVSAYRDAQPEMVPRRPVIRPIPVDESGFAVESDGHGGFVVRGTRPERWINQTN
                     FDNDEAVGYLGDRLARLGVEDELLRLGAKPGCAVTIGDMTFDWEPQTPAGVDVTPTGR
                     GTDGRLDRNDRVGADERKAARRQRREHKE"
     gene            complement(2774..3040)
                     /gene="rpmA"
                     /locus_tag="VFC01_17120"
     CDS             complement(2774..3040)
                     /gene="rpmA"
                     /locus_tag="VFC01_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZN81388.1"
                     /translation="MAHKKGASSSRNGRDSAAQRLGVKRFGGQIVKAGEILVRQRGTH
                     FHPGVNVGRGGDDTLFATAPGAVEFGTKRGRKTVSVVRPSAPQA"
     gene            complement(3054..>3257)
                     /gene="rplU"
                     /locus_tag="VFC01_17125"
     CDS             complement(3054..>3257)
                     /gene="rplU"
                     /locus_tag="VFC01_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZN81389.1"
                     /translation="LPVALVVDGAKVTTDAKALEKVAVTGEVLEHTKGPKIRIHKFKN
                     KTGYHKRQGHRQQLTVLKVTGIK"
BASE COUNT          498 a         1162 c         1074 g          523 t
ORIGIN      
        1 ccacacggcg accgccaggt cagaatggct cgtacgctga atctcgtacg ctgaatcttc
       61 actggggcct cccacatctg tggctctacc ggccaggacc catttcctgt cggcaaccca
      121 cgttcacatg tgagcgaggc cccagccccc cataccgtct agacggcgac caaatcgtcg
      181 gcgtgtacgg cgggccggcg catttcggcg ggcagatccg acgtcgactg gcccagcatc
      241 gtggccagtt cagcggcgtc gtaggccacc acaccgcggg ccaccatcgt cgcgtcctgt
      301 gtgcgcagct cgacgacgtc gccgccgtag aaccgtcccg acagccccgt aatgcccgcg
      361 ggcagcagcg aacgtcgttg ccgcacaacg gcacgcaccg ctccgtcgtc aagcgtcagc
      421 gcgccagcag actcggcggc gtagcggacc cagaacctgc gcgccgacat gcgttcggcc
      481 cgcggcgcga acaccgtgcc gaccgacgcg tcggccagcg ccgtggcggc atcggatgcg
      541 gcggccaaca acaccggcac gccggcatcg gctgccagca acgcagacga cagcttcgag
      601 gccatgccgc cggtgcccag atggctaccc tggcctgcca ccacaccgtc gaggtcggcg
      661 gggcctgcga cctcagggat gaagcgcgcg ttgccttttc gcggatccga gtcgtagagc
      721 ccgtcgatgt cggagagcag caccagcgcg tcggcgccca ccagatgggc aaccaacgcg
      781 gaaagccggt cgttgtcgcc gaaccggatc tcgttggtgg ccacggtgtc gttctcgttg
      841 acgatcgcga cggcgtgcag cgcccgcagc cggtccagcg tgcgctgggc gttcgtgtgc
      901 gacacccgtc gcgagatgtc gtgcgcggtc agcagcacct ggcccactgt gcggtggtag
      961 tgggcgaacg ccgtgctcca cgagttcacc agcgccacct gcccgacgct ggccgcagcc
     1021 tgtttggtgg ccagatcggt gggccgcttg gacagccgca gcggttcgat ccctgccgcg
     1081 atcgaccccg acgacacgat caccacgtcc gagccggcct tcatccggcc ttcgatcgcg
     1141 tcggccaaca cggccaacct gctcgcgtcg aacaccccgg acggtgtagt cagcgcggtg
     1201 gtaccgacct tgatgaccac gctgcgcgcg gtgcgaacgg cctcccgatg ctggctggtc
     1261 attctttgtg ctctcgacgt tggcgtctgg cagctttccg ctcgtcggcc cccacccggt
     1321 cgttgcggtc caaccgcccg tcggtgccgc gcccggttgg cgtcacatcc acacccgccg
     1381 gcgtctgggg ctcccagtcg aaagtcatat cgccgatggt caccgcgcag ccgggcttgg
     1441 cgccgagacg cagcagctcg tcttccacgc cgagccgggc cagccggtcc ccgaggtagc
     1501 ccacggcctc gtcgttatcg aaattggttt ggttgatcca tcgttcgggc cgcgtgccgc
     1561 gcacgacgaa gccaccgtga ccgtcggatt cgaccgcgaa gccgctctcg tccaccggaa
     1621 tcgggcggat gactgggcga cgcggcacca tctccggctg cgcgtcgcgg tacgccgaca
     1681 ccatgtccca cagggcaaac gtcaacggcc gcaagccttc atgggtgacg gtcgacacct
     1741 catacaccgg ccaaccaaag cgccggacga tctcctcgcg cacgaagtcg gccagctccc
     1801 gcgcctccgg cacgtcgatc ttgttgagca ccaccgcccg cggccgctcg gccaggtctc
     1861 ccagtgtcga atcgccttgc agcgtcggcg tatacgcggc cagctcggcc tccagtgcct
     1921 cgatgtcgga gatcggatcc cggccgggtt ccaaggtcgc gcagtcgacg acgtgcacga
     1981 gcaccgcgca gcgttcgatg tgccgcagga aatccaggcc aaggccgcgg ccgtcggacg
     2041 caccggggat caggccgggc acatccgcga cggtgaaggt gtggtcgccc gccgacacca
     2101 cgccaaggtt gggcgccagc gtcgtgaacg ggtagtcggc gatcttcggc ttggccgccg
     2161 agatggccga caccaacgac gacttgcccg ccgacggaaa tccgaccagg ccgacgtcgg
     2221 cgacggtctt gagttccagc gtgaggtcgc ggacctgccc cttttcgccg agcagcgcga
     2281 agccgggcgc cttgcgggcc cgcgacgcca gcgcggcgtt gccgagtccg ccgcgtccac
     2341 cggcggcggc ttcgaatcgg gtgcccgcgc cgacgaggtc ggcgagcagc ctgccgttgt
     2401 cgtcgagcac cacagtgccg tcgggaacct tgacctccag gtcggcaccc gcggcgccgt
     2461 ctcggttgct gcccgcaccc tgcttgcccg acggcgcgac gatgtgtggg tggaagtgga
     2521 aatcgagcag ggtgtgcacc tgcgggtcga cgatcaagac gacactgccg ccgcggccgc
     2581 cgttgccgcc gtcgggtccg ccgagcggtt tgaacttctc gcgatggacc gaggcgcagc
     2641 cgttgccgcc gttacctgcc cgcgcgtgga tgacgacgcg gtctacgaag cgcggcattg
     2701 cgatgaacct ccgaatgtaa cgcccacgcg ggaaagggct tgcgatcccg ccgtgtcgtt
     2761 acagtcgcga agcctaggcc tggggagcgg acggtcgcac cacgctgacc gtcttgcggc
     2821 cccgcttggt gccgaactcc accgcgccgg gggcggtcgc gaacagcgtg tcgtcgccgc
     2881 cacggccgac attcacgccg gggtggaagt gggtgccgcg ctgacggacc aggatctcgc
     2941 cggccttcac gatctggcca ccgaaccgct tgacgccgag ccgctgggcg gctgagtcgc
     3001 gaccgttacg tgagctggaa gcgcccttct tgtgtgccat tcgttcgctc cccttacttg
     3061 atgccggtga ccttgaggac cgtcagctgc tgacggtgac cctgccgctt gtggtagccg
     3121 gtcttgttct tgaacttgtg aatgcggatc ttgggaccct tggtgtgctc gagcacctcg
     3181 ccggtcacgg cgaccttctc cagcgccttg gcgtcggtgg tgaccttcgc gccatcgacg
     3241 accagggcga cgggcag
//
LOCUS       DASRJA010000386         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2803065,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000386 DASRJA010000000
VERSION     DASRJA010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2803065"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="VFC01_17130"
     CDS             complement(<1..113)
                     /locus_tag="VFC01_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HZN81390.1"
                     /translation="MTASYAVVGGGISGLVSAYRLRVAAGPDATITLFDPA"
     gene            complement(110..1159)
                     /gene="hemE"
                     /locus_tag="VFC01_17135"
     CDS             complement(110..1159)
                     /gene="hemE"
                     /locus_tag="VFC01_17135"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HZN81391.1"
                     /translation="MTTRRELPESPYLAAATGRKPSRVPVWFMRQAGRSLPEYRELRA
                     KNTMMQACFDAELITEITMQPIRRHGVDAAILFSDIVVPLRASGIELDIVPDVGPVIE
                     HPVRSIADVKAMKTLEPQQVEPIAQAVSQLVSELGDVPLIGFAGAPFTLASYLVEGGP
                     SRHHERTKAMMLGEPETWHALMTTLADVTIAFLQVQVTAGVDAIQVFDSWAGTLSLAD
                     YRGYVQPHSSRVFAALAGAGVPMTHFGVGTAELLGAMSEVGATVVGVDWRTSLTDAAA
                     RVQPGTALQGNLDPVVLLAGFDVAERAVRAVVEDGRRAVDAGAAGHVFNLGHGVLPST
                     DPAVITDVVTLVHSL"
     gene            complement(1184..>2719)
                     /locus_tag="VFC01_17140"
     CDS             complement(1184..>2719)
                     /locus_tag="VFC01_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZN81392.1"
                     /translation="WHSSPPPLRGTEPVRRWSCRLTVDHLGRPVRRRADLVRDQRGGG
                     ETYVIMPERVADAIEEIARPNRDDQQEKPQIQADPALRRPRLLRSTPVAVAAAIVVGA
                     AVAAGWAAFWALGLPALSGRPGLSIAGRLPVRVTAAVVVGVVVGGLLAWAFRPSQTLR
                     RGARTWATAVRRRSPRIPLIAALAAAVALGVGLAALATWWISGDLFEHATPGSGGVVK
                     VSLPAVVGAVIAVALVVLYRRQKDSDRTQFAQRFGAASTQLGDSDTAVRIAGVYAMAA
                     ADESPVFARRQQCIDVLCGYLRMPYEPDSGSNHLSEFVSTTTWSANPPAVNIDETRRQ
                     AMRQNDREVRDTIVRVLAQRLVRTADTSWSGNDFDLTGVLFEDAWFAGARFNGRHVWF
                     DGANFDSDVMFAGATFRARSASFDGAAFNGKDTSFDDARFAGEYVSFRRVGFSSQTTS
                     FGRAAFKCLRASFELPAQWKNVEFEWENPPSGTQPAIPRCITPRPWPPYLVQQDQDDD
                     KDG"
BASE COUNT          470 a          945 c          885 g          419 t
ORIGIN      
        1 tcggccgggt cgaacagcgt gatcgtcgcg tccggccccg ccgcgacccg tagtcggtaa
       61 gccgacacca ggcccgaaat tccgcccccg acaacggcat acgacgcagt cacagggaat
      121 gcaccaatgt cacgacgtcg gtgatcacgg ctggatcggt gctgggaagc acgccgtggc
      181 cgaggttgaa cacgtggccc gccgcaccgg cgtcgaccgc acggcggccg tcttccacca
      241 ccgcgcgcac cgcgcgctcc gctacgtcga acccggccaa caacaccacc gggtcgagat
      301 tgccctgcag cgcggtgccc ggttgcacgc gtgcggcggc gtcggtcagc gaggtgcgcc
      361 agtccacccc caccaccgtc gcacccacct cagacatcgc gccgagcagc tcggcggtcc
      421 cgacgccgaa atgcgtcatc ggcacacccg ccccggcgag cgcggcaaac accctgctgc
      481 tgtggggctg cacgtagccg cggtagtcgg cgagcgacag cgtgcccgcc caggagtcga
      541 acacctgaat agcgtcgacg cccgctgtca cctggacctg caggaacgcg atggtcacgt
      601 cggccagcgt cgtcatcaag gcgtgccagg tttcgggttc gcccagcatc atcgccttgg
      661 tgcgctcgtg gtggcggctg ggaccgccct cgacgaggta ggacgccagc gtgaacggtg
      721 cgcctgcgaa accgatgagc gggacgtccc cgagctccga caccagttgc gacaccgcct
      781 gcgcaatggg ctcaacctgt tgcggctcaa gtgttttcat ggctttcaca tcggcgatgc
      841 tgcgcaccgg atgttcgatc accggtccga catcgggcac gatgtcgagc tcgataccgg
      901 aggcccgcag cggcacaacg atgtcggaga acaggattgc ggcgtccacg ccgtgacggc
      961 ggatcggttg catcgtgatt tcggtgatca gctcggcgtc gaagcacgcc tgcatcatcg
     1021 tgttcttggc ccgcagctcc cggtattcgg gcagcgaccg gccggcctgc cgcatgaacc
     1081 acaccggcac gcggctgggt ttgcggccgg tggctgcggc cagatagggc gactcgggca
     1141 gctcgcgacg ggtagtcatc ggatgcaatg ctgccatgcg gcatcagccg tctttatcgt
     1201 cgtcctggtc ttgctggaca aggtacggcg gccacggtcg cggcgtgatg cagcgcggga
     1261 tcgccggctg ggttccgctc ggcgggttct cccattcgaa ctcgacgttc ttccactggg
     1321 cgggtaactc gaacgatgcg cgcaggcact tgaatgcggc cctgccaaac gacgtggtct
     1381 gactggagaa gccgacgcgt cggaacgaga catactcgcc ggcgaaccgt gcgtcgtcga
     1441 atgacgtgtc cttgccgttg aaggcggcgc cgtcgaagga cgcactgcgg gcccgaaacg
     1501 tcgcacccgc gaacattacg tcggaatcga aattggcgcc gtcgaaccac acgtgcctgc
     1561 cgttgaacct cgcgccggca aaccaggcgt cctcgaacaa cacaccagtg aggtcgaagt
     1621 cgttaccgga ccagctggtg tcggccgtgc ggacaagccg ttgtgccagc acgcgaacga
     1681 tcgtgtcgcg gacctcacga tcgttctggc gcatcgcctg ccgccgcgtc tcgtcgatgt
     1741 tgaccgccgg tggattcgcc gaccacgttg tggtggagac gaattcgctg agatggttgc
     1801 tgcccgaatc cggttcgtac ggcatacgca agtaaccgca cagcacgtcg atgcattgct
     1861 ggcgtcgcgc gaagactggg ctttcgtcgg cggcggccat cgcgtacacg cccgcgatgc
     1921 gaaccgcggt gtccgaatca cccaactgcg tcgacgccgc gccgaatcgc tgcgcgaatt
     1981 gcgttcgatc cgagtccttt tggcgccgat acagcaccac aagcgcgacc gcgatcaccg
     2041 cgcccacgac cgcgggcagc gatactttga cgacgccgcc gctgcccggg gtggcgtgct
     2101 cgaagaggtc gccgctgatc caccacgtcg ctaaggcggc cagtccgaca cctagtgcga
     2161 ccgccgcggc gagcgctgcg atcaacggaa tccgcgggct gcgacgccgc acggcggtgg
     2221 cccacgtccg cgcaccacgc cggagggttt gggagggccg gaaggcccac gcgagcaaac
     2281 caccgacgac gacgccgaca acgaccgccg ccgtcacgcg aacgggcagc cgcccagcaa
     2341 tcgacaggcc gggtcttccc gacagcgcgg gaaggccaag cgcccagaac gccgcccacc
     2401 ctgccgcgac cgcggcgccc acgacgatcg ccgccgccac agccaccgga gtgctgcgca
     2461 gcagccgggg tcgtcgcagc gctggatccg cttggatctg gggcttttct tgctggtcgt
     2521 cgcggtttgg gcgggcgatt tcctcgatgg cgtccgcaac ccgttccggc atgatcacat
     2581 acgtctcacc tcctccgcgc tggtcgcgca ccaaatcggc gcgcctgcgc acaggcctgc
     2641 caagatggtc taccgtcaaa cggcatgacc accgccgaac cggctcagtt ccgcgaagcg
     2701 gtggcggcga tgaatgcca
//
LOCUS       DASRJA010000387         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_249267,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000387 DASRJA010000000
VERSION     DASRJA010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249267"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFC01_17145"
     CDS             complement(<1..760)
                     /locus_tag="VFC01_17145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZN81393.1"
                     /translation="MTQTTMTRESGPDDDVSTPKRRRLLPPILIAVAVLLVAVIATGL
                     LVYKATIDRSLTENLNRGVELPADEASARPPKEAQETGTLNYLLLGSDSRDPGNEGNG
                     RSDTMLLVHLNAKRNKAYIISFPRDMYVNVPGYGKNKINAAFAFGGAPLAVRTVENLT
                     GVRMDHVALIDFEGFIKLTEDLHGVTVTNKTAFSSHGFNYPEGKITIAGEEALWFVRE
                     RKLLPGGDLDRAENQRNVIKAIAQKGLSVRVISDP"
     gene            889..1278
                     /locus_tag="VFC01_17150"
     CDS             889..1278
                     /locus_tag="VFC01_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81394.1"
                     /translation="MSRAEEAARRAALAAAQARGESLQAQKLIDEFVAAAKARAIAPN
                     PLRATLYNGQSVKTDKVGWYLRKNQSLAIGEDGSYYVLTVPGGFRERFSGVKLKATPP
                     PLVVGRGGRDGESGDLADFLKWRLEAG"
     gene            complement(1289..2398)
                     /locus_tag="VFC01_17155"
     CDS             complement(1289..2398)
                     /locus_tag="VFC01_17155"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZN81395.1"
                     /translation="MLISTSGLMWSRIRLAFVGISVVLATAACGSGAVQPPPPVPPSP
                     VPPSPTAPAPPRTPKATAPEGSSVQQLSGEVSDARAMKHLQALQKIADEHGGNRASGT
                     AGYDASVDYVVGVLRRAGFKVSTPTYEADQDRRSSGPQRNVIAQTSTGDAGQVVMIGA
                     HLDSVEDGPGIVDNGSGVATLLEIATQLGDDPSVQNAVRFAFFGGEENGAEGSRAYVE
                     GLSAGNRKKIKLYLNVDMVASPNGGYFVQGGEGRDAETAGPPGSATIGRVLADQLTKT
                     GVKTPEIIEFVGDDESAFIEAGIPVGGAENGDAERKTRQQAKAWGGQARVRYDRCYHD
                     ACDNIDNVNRDVLDHYMRALAGTVAHFATSTAELR"
     gene            complement(2597..>3511)
                     /gene="sbnB"
                     /locus_tag="VFC01_17160"
     CDS             complement(2597..>3511)
                     /gene="sbnB"
                     /locus_tag="VFC01_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnB"
                     /protein_id="HZN81396.1"
                     /translation="GAGDSVNPPSYFLRFPDRPSSRIIALPASIGGPDRVDGLKWISS
                     FPENVAAGIPRASAVLILNDHDTGYPFACLESSIISATRTAASAALAADWLSRSRPRP
                     TRIGFVGTGLIARYIHTFLAGTGWSFNEIGVHDLSAYSAAGFREYLEQVDTTSQVTVH
                     DNAETLIRSSDLVVFATVAAEPHVQEPSWFEHNPLVLHVSLRDLSPQILLASTNVVDD
                     IEHCLKANTSPHLVEQLTGNRDFLLGTLNDVMAGRVTLPADRPVIFSPFGLGVLDLAV
                     GKHVYDTVMSSGDLYVIDDFFHELRRHG"
BASE COUNT          624 a         1107 c         1116 g          664 t
ORIGIN      
        1 ccggatcgga gatcacccgt acgctcaggc ccttctgtgc gatggccttg atcacgttgc
       61 gctggttctc cgcacgatca agatcgccgc cgggcagcaa cttgcgctcg cgtacgaacc
      121 agagggcttc ctcaccagct atggtgatct tgccctcggg atagttgaag ccgtgggagc
      181 taaatgctgt cttgttggtg accgtcacgc catgcagatc ttcggtgagt ttgatgaaac
      241 cttcgaaatc gatcaacgcc acatggtcca tccggacgcc ggttagattc tccacagtac
      301 ggacggccag cggggcgcct ccaaatgcga acgccgcatt gatcttgttc ttgccgtagc
      361 caggaacatt gacgtacatg tcacgcggga acgagatgat gtaggccttg ttccgctttg
      421 cattgaggtg caccagcagc atcgtgtcgc tgcgcccgtt gccttcattt ccgggatcgc
      481 ggctatccga gccgagcagc aggtagttca gtgtgccggt ctcctgcgcc tcctttgggg
      541 gacgagcaga ggcttcatcg gcgggcagtt cgacacctcg attgaggttc tcggtaaggc
      601 tgcggtcgat tgtcgccttg tacaccagca gccccgtggc gatgaccgcg acgagcagga
      661 cggccacggc gatcaggatc ggcggcagca gtcgacgccg tttgggcgtg ctgacatcgt
      721 cgtctggtcc agattcgcgc gtcatggtgg tctgggtcat gggcttcctt ttttggggaa
      781 gagcaaccgg cagtctatgc ggccggtgcg ttgcatcctg gtgatcccac cgatcgttac
      841 ctgactttct gggagccgct gattggtggt ggccgttacg atccgtatat gtcacgcgcc
      901 gaagaagctg cgcgaagggc cgcgcttgca gcagcccagg cgcgtggcga atccctccaa
      961 gcacagaagc tcattgatga gttcgtcgcc gctgccaagg ccagggcgat tgctccgaat
     1021 ccgctgcggg cgacgctgta taacggccag tctgtgaaga cggacaaagt cggctggtac
     1081 ctccgcaaga accagagcct ggccattggt gaagatggct cctattacgt attgaccgtt
     1141 ccgggcggtt tccgcgagcg gttctccggc gtgaagctga aggcgacacc gccgccgcta
     1201 gtggttggca ggggtggccg cgacggcgag tccggtgatc ttgcggactt cctcaagtgg
     1261 aggctcgagg ccggctagct cgaattgatc aacgcagttc ggctgtcgat gtcgcgaagt
     1321 gggcgacggt gccggcgagt gcccgcatgt aatggtcaag gacgtcccgg ttgacgttgt
     1381 cgatgttgtc gcaagcgtcg tggtagcagc ggtcatagcg cacgcgggcc tgtccgcccc
     1441 aggccttggc ttgctggcgc gtcttcctct cagcgtcgcc gttctccgcg ccgccgacgg
     1501 ggatgcccgc ctcgatgaac gctgattcgt cgtctccgac gaactcgatg atctccggag
     1561 tcttcacgcc ggtcttcgtc agctggtcgg cgagcacccg cccgatggtg gccgaacccg
     1621 ggggtcctgc ggtctccgcg tcgcggcctt cgccaccctg gacgaagtag ccgccgttcg
     1681 gggaggccac catgtccacg ttcagataga gcttgatctt cttgcggttc ccggccgaca
     1741 ggccctccac gtaggctctc gagccctcgg cgccattctc ctcgccgcca aagaacgcga
     1801 accgcaccgc gttctgcacc gacgggtcgt cgccgagctg ggtggcgatc tccagcaggg
     1861 tcgccacccc tgagccgttg tcgacgatcc ccgggccgtc ctcgaccgag tcgaggtggg
     1921 cgccgatcat cacgacctgg ccagcgtctc cagtgctcgt ctgcgcgatg acgttgcgct
     1981 gcggcccgct actgcgccgg tcttggtccg cctcgtaggt cggggtgctg accttgaagc
     2041 cggcgcggcg tagcacgccg accacgtaat cgacgctcgc gtcgtagccg gcggtcccgg
     2101 aggcccgatt accgccgtgc tcgtcggcga tcttctgtaa tgcctgaagg tgcttcattg
     2161 ccctggcatc gctcacctcg ccggacagtt gctgcaccga tgacccctca ggcgcggtgg
     2221 ccttgggggt tcgcggcggc gccggcgccg tgggcgaggg cggcaccgga gacggcggca
     2281 ccgggggcgg cggctgcacc gcgcccgacc cgcaggccgc ggtggcaaga acgaccgaga
     2341 tccctacgaa cgcgagtctg atacgcgacc acatcagccc cgaggtcgat atcagcatga
     2401 gcgtctcctt agcgcggcta tgtataacta cacgatgcgc tctgggaacc ggttcatgtt
     2461 gagacaacga ttgtggcggc cacaaggatc cctcgctccc gcatccagcg gccgcgaaga
     2521 cggcggacat ggcggccgag tgactcgccg aagcgctcct cgctccccac gccagtaggc
     2581 agagtcaaat cgagaattag ccgtgtcgcc gtagctcgtg gaagaagtcg tcgatgacat
     2641 acaggtcccc cgaggacatc accgtgtcgt agacgtgctt gccgaccgcg aggtcgagca
     2701 cccctaagcc gaagggtgag aagatcaccg gccgatccgc cggcagtgtc acccggccgg
     2761 ccatcacatc gttcaatgtg ccgagcagga agtcgcggtt gccagtgagc tgctcgacca
     2821 gatgtggcga ggtgttggcc ttgaggcaat gctcgatgtc gtcgacaaca ttggtagagg
     2881 caagcagaat ctgtggcgag aggtcccgca gcgacacgtg cagcaccagc ggattgtgct
     2941 caaaccatga cggctcctgg acgtgtggct cagcggcgac cgtggcgaaa accaccaggt
     3001 cgctggaccg aattaacgtc tcggcattgt cgtgcactgt tacctgactg gtggtgtcga
     3061 cctgttccag atactcgcga aaacccgccg cgctgtacgc cgacaggtca tgcacgccga
     3121 tctcattgaa cgaccagccc gtaccggcca ggaacgtgtg aatgtaacgg gcgatcagac
     3181 ctgtcccgac gaacccgata cgagttgggc ggggcctgct acggctgagc caatcagccg
     3241 ccaaagccgc cgatgccgcc gtccgggtgg cgctgatgat cgagctctcc aggcaggcga
     3301 acggatatcc ggtgtcatga tcattcaaga tcaacaccgc cgacgccctc ggaatcccgg
     3361 cggccacatt ctccgggaaa cttgaaatcc acttcaaccc gtccacccgg tccggtccgc
     3421 cgatcgacgc cggcaacgcg atgatccgag acgacggacg atccggaaaa cgcagaaaat
     3481 acgatggcgg attcaccgaa tccccggcac c
//
LOCUS       DASRJA010000388         7524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3251498,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000388 DASRJA010000000
VERSION     DASRJA010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7524
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3251498"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VFC01_17165"
     CDS             <1..884
                     /locus_tag="VFC01_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN81397.1"
                     /translation="AEQDAALRSGLQQAAPTLDQVSEVFSGVRDALPQTLANLAIVID
                     MLKRYHKGLEQTLVILPQGAAIAQAGTLFDNVGQLPLALSINQPPPCLTGFLPASEWR
                     SPADTSMAPLPSGTYCKIPKDTQANAVRGARNYPCADVPGKRAATPRECRSNEPYVPL
                     GTNPWYGDPNQILSCPAPAARCDQGVNPGRGVVPAPTVNNGLNPLPADQLPPPSASSA
                     PVSDPLSPPGRGTVQCSGQQPNPCTYTPAQGPPGSVAVYSPSSGEVVGPDGIRYNVSN
                     SSKPGEDGWKEMLAPAS"
     gene            854..1510
                     /locus_tag="VFC01_17170"
     CDS             854..1510
                     /locus_tag="VFC01_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81398.1"
                     /translation="MEGDAGTRQLNPTDADETPDESLAVSTEDQTEEATELQAPEPAD
                     EAAGDDRRQGRLGIGWALGICAALLVLAAGAGVGGYFALRSHQDSEAIARADAAALVA
                     AKDCVAATQAPDGAAMLASQSKIIECSTGAFGVQAPLYASVLAEAYQAANVQVKVSDM
                     RAAVERHNDDGSVDILVAFRTQVTNSEAAGTEQGYRLRVQMAPADGTYKIADMKQVAS
                     "
     gene            1507..2481
                     /locus_tag="VFC01_17175"
     CDS             1507..2481
                     /locus_tag="VFC01_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZN81399.1"
                     /translation="MTAVLDAMPDTADDDSAPESPTASWLARAGAFSIDVLLGIAVIA
                     TMALLTLSAPQRGWLWWVLTATAGLIFVLIAVNRVVLPWLTGWSLGRAVFGIAVRKRD
                     GSPVGLARLVGRDLAHLLDTAALLVGWLWPLWDRGRRTFADLLLRTEVHKVDRPQRDA
                     RRPAAVALIVATLLCAAGVGLSYLVVYRHDRAVDTARTQISEEGPRIVEQMLNYDVET
                     LQQDFSHAQSLTTDAYRPQLIAQQQAVQKAGATKNEYFAVSSAVLSATQQHASMLVAL
                     QGQRGSDPKDLKFITATVRVDFDKSRDGQWRVANLTVLKKPQMNQAGQ"
     gene            2478..3029
                     /locus_tag="VFC01_17180"
     CDS             2478..3029
                     /locus_tag="VFC01_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mammalian cell entry protein"
                     /protein_id="HZN81400.1"
                     /translation="MSPRRKLDADEHDFFAVVPKPPRRWGLPIISLLATLLIAAAIAG
                     STLMLIQHVKDHKETVKAVEARDYVREFMTQYTTLDPFHANDYADRILAQGTGDFAKM
                     FKEKQNEIMIQVARAEPTTGTVLEAGVQRWNDNGSADVLVATKMSTKSPDGKSNVESG
                     NRWVATAIKEGQQWKISNLIQVI"
     gene            2996..3955
                     /locus_tag="VFC01_17185"
     CDS             2996..3955
                     /locus_tag="VFC01_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mammalian cell entry protein"
                     /protein_id="HZN81401.1"
                     /translation="MEDQQPDSGDLTTDAQSPDQPADAAKMPTGKAARHRHRMPLRKE
                     LAANREDADDTATDDQASADAEPELETAEAEPAPPRRRWSLFRRRTKTPEPETVSEPA
                     DDVAEPEQAEAEPEPAVDETTEAAEAEVASDAQDAPAEEQILVPHRPAGKRLTIAAAA
                     AGVVFVGAAAFAGATLQPYLADRALVHTKLDIARTAANAITTLWSYTPDDMDRLPDRA
                     AGYLGGDFANEYRKYIDAIVASNKQAQVTNTTQVMGTAVESVSPNEATALVYTNSVAT
                     SPVSKNIPSLRYLSYRLTMQRHDSDWLITRMSTVTSFDLTPQL"
     gene            3973..4665
                     /locus_tag="VFC01_17190"
     CDS             3973..4665
                     /locus_tag="VFC01_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HZN81402.1"
                     /translation="MPVVSPVSERATVVALLLLTFATGLVDAVSVLELGHVFVANMTG
                     NVVFLGFWLAPHTVVDVTAALVSFVSFVAGAVIGGRLARHLDQHVRRWLTVALGVEVV
                     MLAVLSILSGTGVLDYHDNRKLILIAGLAVTFGIQNSTARQFGVQELSTTVLTTTISG
                     LGFDSRLAGGSGEREKLRYSVVLMLCAGAAVGATMTRFTVAPVIAVAAVVVAVSAALF
                     VFGPRRQEVAAG"
     gene            4675..5505
                     /locus_tag="VFC01_17195"
     CDS             4675..5505
                     /locus_tag="VFC01_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN81403.1"
                     /translation="MPSVLVTGAGRGIGKAITEHLAARGWDVIAGVRSERDAEAIAAL
                     APQRISSVILDVTDAGHIAALNESLPERLDAVVNNAGIVVSGPMEAVTPEDWRKQLEI
                     NVIGQLAVTRAVLPRLRASRGRVVFISSVNGRMSMSLVGAYCASKFALEAAADALRME
                     LRPWRIGVAIVEPAQTDTDMWRTADDMAEEAEAALAPEHRALYARHLYGFKKTIPVSQ
                     KLAVPAKKVSAVVEEALTARRPRARYVVGIGPKLQVAVITKLPTSVRDLVLRRVSGQP
                     "
     gene            5505..6284
                     /locus_tag="VFC01_17200"
     CDS             5505..6284
                     /locus_tag="VFC01_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HZN81404.1"
                     /translation="MFVKRSTQAPESFFACEAAGLQWLSAADGGVPCARVIAYDDTSL
                     TLERLDVAAPTRAAAHEFGRRLARTHDAGADAFGAAPRGWTGPGYFGPLQQPLPMSYA
                     ASDRWGSFYANERLAPMAERAAPSLAASARDDIDAVMARCAAGDFDDDDPPARLHGDL
                     WSGNVMWTPGGAVLIDPAAHGGHRETDLAMLALFGCPHFDAVIAGYQEHRPLRDGWRD
                     RVGLQQLYPLLAHVVLFGGGYARQASAAASSALRGYGRAVN"
     gene            complement(6277..7335)
                     /locus_tag="VFC01_17205"
     CDS             complement(6277..7335)
                     /locus_tag="VFC01_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HZN81405.1"
                     /translation="MRRFVACAAAFLVCAAGPVMASPTARAADGRELLAGAIANTRGS
                     YLVYNFGGDAPAPMLNAGGGWYEMNSGGHLMIIKGASTRLAPRLLVDSHSGYQARCER
                     DARARTGEGLWQASEIYTPLQAWQVLGQPTIAINANFFDVRGQKGGSWKSTGCSSPLG
                     AYVDNTRGQGRTNAAVTGTVTYAGKQGLSGGNEHWKALSTMILPVGGAPYVVPPKGPD
                     DYDAASPVVQGLLDKGVRFVAVSGIGLLAPGDTGQLNDGGPSAARTAIAYAKGKDEMY
                     VFQGGSYTPDQIQDLFRGLGSDTAILLDGGGSSAIVLRRDTGGMWAGAGVPDGNCDTR
                     QVLCDSRERALPSWLAFN"
BASE COUNT         1298 a         2443 c         2575 g         1208 t
ORIGIN      
        1 ccgccgaaca ggacgcggca ctgcgcagcg ggctgcagca ggccgcaccc acgctggacc
       61 aggtgtcgga agtcttcagc ggtgttcgtg atgccttgcc gcaaacgctg gccaacctgg
      121 ccatcgtcat cgacatgctc aagcggtacc acaagggcct ggagcagacc ctggtgattc
      181 tgccgcaggg cgctgcgatc gcgcaggctg gaacgctctt cgacaacgtt ggccaactcc
      241 cgttggcgct gtcgatcaac cagccgccgc cgtgtttgac cgggttcctg cctgcgtcgg
      301 aatggcgatc accggccgac accagcatgg cgccgctgcc gtcgggcacc tactgcaaga
      361 tcccaaagga cacccaggcc aacgccgttc gtggtgcgcg taactatccg tgtgcggatg
      421 tcccaggcaa gcgcgcggct acaccgaggg aatgccgcag caacgaaccg tacgtgccgc
      481 tgggcaccaa cccgtggtac ggcgacccga accagattct gtcgtgcccg gccccggcag
      541 cccgctgcga ccagggagtc aatcccgggc gaggcgtcgt cccggcaccg acggtgaaca
      601 atggattgaa cccgctaccc gcggatcaat tgcccccgcc gtcggcgtcg tcggcaccgg
      661 tcagcgaccc gctgtccccg cccgggcgcg gtacggtcca gtgcagtggg cagcagccga
      721 acccgtgtac gtacactccg gcacaaggac cacccggttc cgtggcggtc tacagtccgt
      781 ccagcggtga ggtggtcgga cctgacggca tcaggtacaa cgtcagcaat tcgagtaagc
      841 caggagaaga cggatggaag gagatgctgg cacccgccag ctgaacccca ccgatgctga
      901 tgaaacgccg gatgagtcgt tagcagtatc tacagaagac caaaccgaag aagccaccga
      961 actccaagca ccagagccag ccgatgaggc cgccggggac gatcggcggc aggggcgcct
     1021 cggcatcggc tgggcgctgg ggatctgcgc cgcgctgctc gtcttggctg cgggggcggg
     1081 cgtgggtggc tactttgcac tgcggtccca tcaggacagc gaggcgatcg cgcgcgccga
     1141 cgcggccgcg ttggtagccg ccaaggattg cgtggcggcg acgcaggccc cagatggcgc
     1201 cgccatgctc gccagccagt ccaaaatcat cgagtgctcg acgggtgcct tcggtgtgca
     1261 ggcccctctg tacgccagcg tgctggccga ggcctaccag gccgccaacg tccaggtgaa
     1321 ggtgtcggac atgcgcgccg cggtggagcg gcacaacgac gacggttcgg tcgacatcct
     1381 ggtggcgttc cggacgcagg tgacgaactc ggaggcggca ggcacggagc agggttatcg
     1441 gctacgggtg cagatggcac cagccgacgg cacctacaag atcgccgaca tgaaacaggt
     1501 cgcctcgtga cggcagtgct cgacgcgatg ccggacacgg ccgacgacga ctcggcaccc
     1561 gagtcgccga ccgcgtcgtg gttggcgaga gccggcgcgt tctcgatcga cgtgctgttg
     1621 ggcattgcgg tgatcgccac gatggcgctg ctgaccctgt ccgcgccgca gcgtggctgg
     1681 ctgtggtggg tgttgacagc cacggcgggg ctgattttcg tgctgattgc ggtcaatcgg
     1741 gtagtgctgc catggctcac cgggtggtcg ctgggccgtg cggtgttcgg catcgccgtg
     1801 cgcaagcgcg atggctcgcc ggtgggtctc gcgcgactgg ttggtcgtga ccttgcccat
     1861 ctgctcgaca ccgccgcgct gttggtcgga tggctgtggc cgctgtggga tcgcggccga
     1921 cgcaccttcg ccgatctgtt gctgcgcacc gaagtacaca aggtggaccg cccgcagcgc
     1981 gacgcgcgaa ggcctgccgc ggtggcgctc attgtggcga cgctgttgtg cgcggcgggg
     2041 gtggggctga gctatctggt ggtgtaccgg catgaccgag cggtcgacac ggctcgcaca
     2101 cagatttcgg aggaggggcc gcgcatcgtc gagcagatgt tgaactatga cgtcgagacg
     2161 ttgcagcagg acttctccca cgcgcagtcg ctgacgaccg acgcctaccg gccgcagttg
     2221 atcgcccagc agcaggcggt gcagaaggcg ggcgccacca agaacgagta tttcgcggtg
     2281 agcagtgcgg tgctctcggc gacgcagcag cacgcgtcga tgctggtggc actccagggc
     2341 cagcgtggca gcgaccccaa ggacctgaag ttcatcaccg cgacggtgcg ggtggacttc
     2401 gataaatcac gcgacgggca gtggcgggtc gcgaatctca ccgtactgaa aaagccgcag
     2461 atgaatcagg ccggtcaatg agtccgcggc gcaagctcga cgccgacgag catgatttct
     2521 tcgctgtggt gcccaagccg ccgcggcgct ggggtctgcc gatcataagc ttgctggcga
     2581 cgctgttgat cgcggcagcg atcgccggca gcacgctcat gctgattcag catgtgaagg
     2641 atcacaagga aacggtcaag gcggtcgagg cgcgggacta cgtgcgcgag ttcatgaccc
     2701 aatacacgac gctggatccg ttccacgcca acgactatgc ggatcgaatc ctcgcccagg
     2761 gcaccggcga cttcgcgaag atgttcaagg agaagcagaa cgagatcatg atccaggttg
     2821 cgcgcgccga gcccaccaca ggcactgtgc tcgaggcggg cgtgcagcgg tggaacgaca
     2881 acggcagcgc cgacgtgctg gtcgcgacga agatgtccac gaagtcgccg gacggcaagt
     2941 cgaatgtcga aagcggaaac cgttgggttg caacggctat caaggaagga cagcagtgga
     3001 agatcagcaa cctgattcag gtgatctgac caccgacgcc caatcaccgg atcagccggc
     3061 ggatgctgcg aagatgccga ccggtaaggc ggcccgtcac cgccatcgga tgccgcttcg
     3121 caaagagctt gcagcgaacc gtgaggacgc ggacgacacc gcgaccgacg atcaggcctc
     3181 ggccgacgca gagcccgagc tcgagaccgc cgaagccgaa cccgcgccgc cacggcggcg
     3241 gtggagcctg ttccgtcggc gcacgaaaac acctgaaccc gaaactgttt cagagccggc
     3301 ggatgacgtg gcggaaccgg agcaggcgga ggccgagccc gagccggcgg tcgacgagac
     3361 cacggaggcc gcggaagccg aagtagcctc tgacgcccag gacgcgccgg ccgaggaaca
     3421 gattctggtg ccgcaccgtc ccgcgggcaa gcggctgacc atcgcggcgg ccgccgccgg
     3481 tgtcgtgttc gtcggtgcgg cggcgttcgc gggtgcgacg ctgcagccgt acctcgccga
     3541 tcgtgccttg gtccacacca agctcgacat cgccaggacc gccgccaacg cgatcaccac
     3601 gctgtggagc tacacgccag acgacatgga caggctgccg gaccgggccg ccggctactt
     3661 gggcggcgac ttcgccaatg agtaccgcaa gtacatcgat gcgatcgtcg cttcgaacaa
     3721 gcaggcgcag gtcaccaata ccacgcaggt gatggggacg gcggtcgaat cggtgtcgcc
     3781 gaacgaggcc actgcactgg tctacaccaa ctcggtggcc accagcccgg tgtcgaaaaa
     3841 cattccgtcc ctgcggtacc tgtcctaccg gctgacgatg cagcggcacg actcggactg
     3901 gttgatcacc cgcatgtcga cggtgacgtc ttttgatctg accccgcagc tgtagcggcc
     3961 agacgcgcgg tcatgcccgt tgtctctccc gtctctgaac gggcgacggt tgttgcactg
     4021 ctgctgctca cgttcgcgac cggactcgtc gatgcggtca gcgtgctgga gctcggccat
     4081 gtgttcgtcg cgaacatgac gggcaacgtt gtcttccttg gtttctggct cgctccgcat
     4141 accgtcgtcg acgtcaccgc cgccctagtc tcctttgtga gcttcgtggc gggcgcggtc
     4201 atcggcggac gcctggcgcg gcacctcgac caacatgtgc ggcgctggct gaccgtggcg
     4261 ctcggtgtcg aagtggtgat gctcgcggtg ctctcgattc tcagcggaac gggtgtgctc
     4321 gactaccacg acaacagaaa actgattctg atcgcgggcc tggcggtcac gttcgggatc
     4381 cagaactcca ctgcgcggca gttcggcgta caagagctat cgaccacggt cctgacgacc
     4441 acaatctcgg gactcggatt cgacagccga ttggccggcg gctccggtga acgcgagaag
     4501 cttcgctaca gcgtggtgct catgctgtgt gcgggtgccg cggtcggcgc gacaatgacc
     4561 cggttcaccg tcgcgccggt gattgcagtt gctgcggtcg tggtggccgt cagcgctgca
     4621 ctcttcgtat tcggcccgcg tcggcaggag gtggccgcgg gatagtctcg ctgtatgcca
     4681 tcagtactcg tcaccggtgc aggacgtgga atcggcaagg ccatcaccga gcacctggct
     4741 gcccgtggct gggacgtgat cgcgggggtc cgcagcgaac gtgatgccga ggcgatcgcc
     4801 gcgttggccc cacagcgcat ctcctcggtc atcctcgacg tcaccgatgc cggacacatc
     4861 gccgcgctga acgaatcgct gcctgagcgg ctggacgcgg tcgtcaacaa cgcgggcatt
     4921 gtggtgagcg ggccgatgga agccgtaacg cctgaggact ggcgaaagca gctggaaatc
     4981 aacgtgatcg gtcagctggc cgtcacccgg gcggtgctgc cgcggctgcg agcgtcgcgg
     5041 ggacgggtgg tgttcatctc cagcgtcaac ggccgcatgt cgatgtcgct cgtcggcgcg
     5101 tactgcgcca gcaagttcgc cttggaagcg gccgccgacg cgctgcgaat ggagttgcgg
     5161 ccgtggcgca ttggcgtcgc gatcgtcgag cccgcgcaga cggacaccga catgtggcgt
     5221 accgcagacg acatggccga ggaggccgag gccgcactgg caccggaaca ccgagcgctg
     5281 tacgccagac atctgtatgg cttcaagaag acgatcccgg tgtcccagaa gctggccgtc
     5341 cccgccaaga aggtgtccgc ggtcgtggag gaggcgctga ccgcccgtcg gccccgcgcc
     5401 cggtacgtcg tcggtatcgg tcccaaactg caggtggcag tgatcacgaa gctgccgacc
     5461 tcggtgcgcg acttggtgct gcgacgtgta tccggtcagc cctagtgttc gtcaagcgca
     5521 gcactcaagc gcccgagagc ttctttgcct gtgaagccgc tgggctgcaa tggctttcgg
     5581 ccgccgacgg cggcgtgcca tgcgcccggg tgatcgcata cgacgacacc agcctgacac
     5641 tcgaacggtt ggacgtcgct gcgccgaccc gggctgccgc gcacgagttc ggccgtcggc
     5701 tggcccgcac gcacgacgcc ggggcagacg cgttcggcgc agcgccccgc ggctggaccg
     5761 gtcccggcta cttcgggccg ctgcagcaac cgctgccgat gtcgtacgcc gccagcgacc
     5821 ggtggggcag tttctacgcg aacgagcggc tcgcaccgat ggccgagcgc gcggcaccga
     5881 gcctggccgc gtcggctcgc gacgacatcg acgcggtgat ggcgcgctgc gctgcaggcg
     5941 atttcgacga cgacgatccg cccgcccggc tgcacggtga cctgtggagc ggaaacgtga
     6001 tgtggacgcc cggtggtgcg gtgctgatcg acccggcggc gcacggcgga caccgcgaga
     6061 cggacctcgc gatgttggcc ctgttcggct gtccgcactt cgatgcggtg atcgctggct
     6121 atcaggagca ccggccgctg cgcgacggct ggcgagaccg cgtcgggctg cagcaactct
     6181 acccgctgct ggctcacgtc gtgctgttcg gcggcgggta cgccaggcag gcgtcagccg
     6241 ccgcgtcgag cgcgttgcgt ggctacggcc gagccgtcaa ttgaacgcca gccagctcgg
     6301 cagcgcgcgc tcacgcgagt cgcacaacac ctgccgcgtg tcgcaattgc cgtccggcac
     6361 accggcaccg gcccacattc cgccggtgtc gcggcgcagc acgatcgccg aggaaccgcc
     6421 gccgtcgagc aggatcgcgg tgtcgctgcc gaggccgcgg aacagatcct ggatctgatc
     6481 cggcgtgtag ctgccgccct ggaagacgta catctcgtcc ttgcccttgg cgtacgcgat
     6541 ggccgtgcgg gccgcgctgg ggccgccgtc gttgagctgc ccggtgtcgc cgggggccaa
     6601 cagaccgatg ccgctcaccg cgacaaaccg cacgcccttg tcgagcaggc cctgaaccac
     6661 cggcgacgcg gcgtcgtagt cgtccgggcc cttcggcgga acgacgtacg gcgcgccgcc
     6721 gaccggcagg atcatcgtcg acagcgcctt ccaatgttcg ttgccgccgg aaagcccttg
     6781 cttgcctgca taagtcacgg tgccagtgac cgcggcgttg gtgcgaccct gtccgcgggt
     6841 gttgtcgacg taggcgccca gcggagagct gcagcccgtc gacttccatg acccgccctt
     6901 ctggccgcgg acgtcgaaga agttggcgtt gatcgcaatc gtcggctgac caaggacctg
     6961 ccatgcctgt agtggcgtgt agatctccga ggcttgccac agcccttcgc cggtgcgtgc
     7021 ccgcgcatct cgttcgcagc gagcctgata tccgctatgc gaatcgacga gcagccgagg
     7081 ggcaagccga gtcgacgcgc ccttgatgat catcaggtgg ccgccgctgt tcatctcgta
     7141 ccagccgccg cccgcgttca gcatcggagc cggtgcgtcg ccgccgaagt tgtagaccag
     7201 gtaggagcca cgggtgttgg cgatcgcgcc ggccagcaat tcacggccgt cggcggcgcg
     7261 ggcagtcggg ctggccatca cgggccccgc ggcgcacacc agaaacgccg cggcgcatgc
     7321 gacgaaacgc cgcatgtttg cgcgtcgagt tggcacggtc gcccctttgc cgagacagct
     7381 aagaagacaa cagtaatcac agaagacaca cagtcaactt tggtaacagc tgagcatcag
     7441 ttgggcgtca ggtggatcgc atcgttagct gagcccgagt ggaattcggt ggaacgggta
     7501 atcccgcggt ggtcggggtt ctaa
//
LOCUS       DASRJA010000389         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1254340,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000389 DASRJA010000000
VERSION     DASRJA010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254340"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            434..1681
                     /locus_tag="VFC01_17210"
     CDS             434..1681
                     /locus_tag="VFC01_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81406.1"
                     /translation="MSCVWILMPAMAAAQVRDQPREVTVNAVSLRGTIQAIDQTARTV
                     VIKGDKGNLVTLDVPPSVTRLDEVKVGDIVTITYYDRVSLRLKPAGEAAVDRVVEPTT
                     MATAGALPGATRSRQRIATATITAWDPATRQVDFTGPKGNTYSRYVATTIDPSVLAGI
                     KVGDRVDVTWTEALSLQVSSATPAADPDDFRHRVTFSVQFGIDNQFSGKLIKEATGTR
                     TNGAPINLNETTFDDVYGRIGMFKIGAGYRTSPRSEGVFNFVWSKSDAQESAILVGTA
                     GTSQVPLNVTFSAYEYWGFEGGQRFYFARTRFTPYLGYLVGINRFQEIFGTFAGVPPP
                     DTPVLANTDGSIFDNSWAISLGPTGGVLIGVGPFEVMGEVQLRYIGGLSDVDWLVEEG
                     LSDVNDESSRWSFPLMIGARIRF"
     gene            complement(1738..>2276)
                     /locus_tag="VFC01_17215"
     CDS             complement(1738..>2276)
                     /locus_tag="VFC01_17215"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZN81407.1"
                     /translation="GAVLVRVETPFGNIDMAIDVERAPITAANFLKYVDGGFYDGGRF
                     HRATRADNYKPNLPNRPLLEIIQADISAARSEERFPAIPLERTNRTGLSHVVGTVSMP
                     RGEAADSARSGFVIHFNDQPSLNYGGMRFDDGQGTAAFGRVIAGMDVARSIQQQPVTE
                     QALTPPVTITRIFRVTPR"
BASE COUNT          418 a          712 c          730 g          416 t
ORIGIN      
        1 tcgctgcagt gcaccacgat gacgccggcc tctggcggga taccgtgagg agaggtgcgg
       61 taaacgacgg ccacacggga cagcttagca agaccgatcg accggcgtcg acacgacgca
      121 tacggcgtgc atagccgaaa cctcaacgct cctttgtcag ccggcctccg agctaccgga
      181 cctctctccg aaatcccggg ctaaagaaac ccggacagtt gcggacctct tgaacgcccg
      241 tcccggcaaa cggctcgcac ggcttcggtt cctacgttga acgcacctgc gaagcaccgg
      301 gcatatctgt tgctccctcg ccaatcgcat tgctcagcaa ggatgcgcga caccccggct
      361 caggcgccac gggtcgttct cggtaattcg gcgccgcaca ggtgatgacg tggtgaaacg
      421 attattcgtc tgcatgagtt gcgtttggat cctgatgccg gcgatggcgg cagcccaagt
      481 ccgtgaccag ccccgtgaag tgacggtcaa cgcagtctcg cttcgaggga caatccaggc
      541 catcgatcag actgcccgca ccgtggtgat caaaggggac aagggcaacc tggttacact
      601 ggacgtcccc ccaagcgtca cgcggttgga tgaggtgaag gtcggcgaca tcgtgactat
      661 cacctattac gaccgcgtga gcttgcggtt gaagccggcg ggcgaggctg ccgtcgaccg
      721 cgtggtcgag ccgacgacga tggcgaccgc cggggccctg ccgggcgcaa cccgctcccg
      781 tcagcggatc gcgacggcga ccatcacggc ctgggatccg gccactcgcc aggtggactt
      841 caccgggccc aagggcaaca cctactcacg ttatgtagca acgacgatcg atcccagcgt
      901 cctcgcgggc atcaaggtcg gcgatcgcgt cgatgtcacg tggactgagg cgctgtcgtt
      961 gcaggtcagc tcggcaacgc ccgccgcgga ccccgatgat ttccggcacc gggtcacgtt
     1021 ctccgtgcag ttcgggatcg acaaccagtt cagcggcaag ctgatcaagg aggccaccgg
     1081 gacgaggacc aacggcgcgc caatcaacct taacgagacc acattcgatg acgtgtacgg
     1141 gcggataggg atgttcaaga tcggtgcagg gtaccggacg agcccgcgct ccgagggggt
     1201 gttcaacttt gtctggtcca agagcgatgc gcaggagtca gccattctgg tgggcaccgc
     1261 aggtacgtcg caggttccgc tgaatgtcac cttcagcgca tacgaatact ggggcttcga
     1321 aggtggccag cggttctact tcgcacggac ccgcttcacg ccctacctgg gctatctcgt
     1381 cggcattaat cggttccagg aaattttcgg gacgttcgcg ggcgtgccgc ccccggacac
     1441 gcccgtactc gccaacacgg atggctcgat cttcgataat tcgtgggcta tcagcctggg
     1501 accgaccggc ggcgtgctca tcggcgttgg ccccttcgag gtgatgggtg aggtccagct
     1561 tcgttacata ggcggcctgt ccgatgtcga ctggctcgtc gaagagggcc tcagcgacgt
     1621 caacgacgag agctcgcgtt ggtcattccc cctcatgatc ggcgcgagaa ttcggttctg
     1681 atcgacgcat gctgcgctgg gaccgtgcgc ggcccctccg ggtggggccg cgcgccttca
     1741 gcggggtgtc actcgaaaga ttcgggtgat cgtgaccggc ggggtcaacg cctgttcggt
     1801 gaccggctgc tgctggatcg agcgcgcaac atccatccct gcgatcacgc gtccgaaagc
     1861 agccgttccc tggccgtcat cgaaccgcat tccgccataa ttcagcgacg gctgatcgtt
     1921 gaagtggatg acgaagccgg accttgccga atcagcggcc tcgccacgtg gcatggacac
     1981 ggtgcccacg acgtgcgaca ggccggtcct gttcgtacgc tcgaggggaa tggccgggaa
     2041 ccgctcctcg ctccgcgccg cgctgatgtc ggcctgaata atttcgagca ggggccggtt
     2101 cggcaggttg ggcttgtaat tgtcggcgcg cgtagcgcgg tggaagcgtc cgccgtcgta
     2161 gaagccgccg tccacgtatt tgagaaagtt ggcggcagtg atcggtgcgc gttcgacatc
     2221 gatcgccatg tcgatgttgc cgaacggtgt ctcgacccgc acgagtacgg cacctg
//
LOCUS       DASRJA010000390        12114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_351461,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000390 DASRJA010000000
VERSION     DASRJA010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351461"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..766)
                     /locus_tag="VFC01_17220"
     CDS             complement(50..766)
                     /locus_tag="VFC01_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZN81408.1"
                     /translation="MTTEANVGALHDNLLTALGASIVSGEYPAGQVLTLEGVSAHHGL
                     SRSVAREAIRVLESMGMVASRRRVGITIQPAEKWNVFDPRLIRWRLEAGDRAAQLVSL
                     SELRRGFEPAAAALAARRADPHQCRIMAAAVSDMVMHGRSGDLEAYLLADKIFHQTML
                     EASGNEMFRALNGVVAEVLAGRTHHGMMPDLPNPAAIELHDEVARAIRMRDEEAAERA
                     MRAIIDEAASAVAEEFSEPS"
     gene            890..1405
                     /locus_tag="VFC01_17225"
     CDS             890..1405
                     /locus_tag="VFC01_17225"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HZN81409.1"
                     /translation="MASPIVVMGVSGSGKSTVGAALAQRLRVPFADADDFHPPANIAK
                     MTAGQPLDDDDRHPWLDAIGEWLAAHGDGGVMSCSALKRKYRDQLRRHCPDVEFLHLS
                     GTPEVIGRRQASRPGHFMPASLLASQFATLEPLEPDERGIVIDVDQDIDSVVDNYVAL
                     SATRTTEQENR"
     gene            1402..2883
                     /locus_tag="VFC01_17230"
     CDS             1402..2883
                     /locus_tag="VFC01_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HZN81410.1"
                     /translation="MTPALTILAADTELPQPIAGGGQLVAAALAGIAIIVVLITVTKL
                     HPFLALIFGALTVGIVAGFPSLDTFDDTLSSFSDGFGTTAAGVGILIALGAMFAKLLA
                     DSGGADEIVDTIVGHASPRTLPWAMALVGAIIGLPMFFEIGLVLLIPVIYLVAKRSQL
                     SLITVGIPALAGLSAMHGLVPPHPGPLTAIDLLGADLGITLALGVLVAIPTVIVAGPL
                     FGKLAGRWVVVDVPDRFEADDFARNGGGAVTTAAVGAEGEGTTTQTTTRVERRRPSFG
                     ITLFSVLLPVGLMMGKALVDIFIDDETNALRQIFDIVGRPLMALLIAVIVGIFTLGRG
                     AAMTRDQIVKCIETSLPPVAGIILIVAAGGGFKQVLVDSGIGTLLADWAKGANVSIIL
                     LAWVLAVLIRLATGSATVATITASSLMLGLVEGLSTGEVSLVVLAVGAGSVFFSHVND
                     AGFWMVKEFFGMTVGQTIKTWSLMETVLSVAGLVLVLLLGIVI"
     gene            complement(2961..3452)
                     /locus_tag="VFC01_17235"
     CDS             complement(2961..3452)
                     /locus_tag="VFC01_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZN81411.1"
                     /translation="MTSGDFQPQPGQYGEPGGYPPPGGQEPGGLLLRFIARFIDGIIV
                     NIVAFAIALAIGSVTNYWVTGLFSGLLMFLYFLAFESTQGWTPGKKLLGLSVRGPGGA
                     PKPDVQQAAIRNVWTLLNIIPFIGGLLTLIAVIVIAVTINSSPTKQGKHDELAGGTQV
                     IKG"
     gene            complement(3544..4398)
                     /locus_tag="VFC01_17240"
     CDS             complement(3544..4398)
                     /locus_tag="VFC01_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HZN81412.1"
                     /translation="MLIRRIARPMLSAVFISRGVDALRSPKPAADAARPTLEGLSKLP
                     DPVGTNVPSDAEKVARINGAVQIGGGLLLASGKLPRVASAALALTVVPGSLGGHTFWS
                     ETDPQRKADERRAFLTDVSLIGGLIIAAVDTEGKPSLGWRGRRAAHKVSEAVTAALPV
                     GAATGGAVADSALAEKVGHGLQVGAERGRELAHVARERGAEFADVARERGAEWAEVAR
                     ERAPVLAEAARERGAEIAELARERGAEWAEVARERAPELADTARERAGELTDTARAQV
                     KHRRSRFS"
     gene            complement(4435..4944)
                     /locus_tag="VFC01_17245"
     CDS             complement(4435..4944)
                     /locus_tag="VFC01_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HZN81413.1"
                     /translation="MDDIEHPEAQEKWDTDRDETEAQRLDRNWSSLLQELRVAQTGVQ
                     LLTGFLLTLPFQQRFTQLDGVMRTVYLITVACSIGATVLLVAPVSMHRLLFRRHRLKT
                     LVSAAHSYAMVGMVLLGIALAGVAVVIFDTVVGRTGAWISGACTLLALVAFWYVMPIR
                     GRKDKDNRY"
     gene            complement(4960..5508)
                     /locus_tag="VFC01_17250"
     CDS             complement(4960..5508)
                     /locus_tag="VFC01_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011594728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZN81414.1"
                     /translation="MRKLVVTQNITLDGSIEMLDDWFDPQLQDEDLLEESHRQDAQAD
                     ALLVGRQTFEDFRGYWPKQTDDATGVTDYLNRVAKYVVSTTMSDPQWENSTVLNGDAV
                     ALTTALKVQPGREIVLTGSISLAHTLIAAGVVDEYRLFVYPAVQGRGRRLFPDGTGIA
                     RLTLVEPPKSFRSGITLLRYAV"
     gene            5564..6961
                     /locus_tag="VFC01_17255"
     CDS             5564..6961
                     /locus_tag="VFC01_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HZN81415.1"
                     /translation="MTLVAGIDSSTQSCKVVVCDADTGEIVRSASSPHPDGTEVDPQL
                     WWSALQSSIGAAGGLDDVAAVSVGAQQHGMVCLDGAGAVVRDALLWNDTRSGAAAAEL
                     VDELGGPAEWAKRIGVVPVAAITATKLRWLADHEPAHADATAAVCLPHDWLTWRLSDS
                     TDITTLHTDRSDASGTGYFSSEADSYQPDLLELAMRGRRPALPTVLGPRESAGQASTG
                     ALLGPGAGDNAAAALGLGAGTGDCVVSLGTSGVVSAVGEAAPHDPEGIVAGFADATGR
                     QLPLVCTLNGAPVLAAVTTMLGIDFDELDRLALSAPAGADGLTLAPYFEGERSPNLPS
                     AAGALHGMTTRNLLPANVARAAVEGLLASMAYCVDKISAQGIDVQRIILIGGGARSEA
                     VRRIAPAVLGAPVHVPTPAEYVALGAARQAAWTLSQQDSPPSWSFGVTASYSADPTPE
                     VLEQYRGAQRLTLGQ"
     gene            6958..7650
                     /locus_tag="VFC01_17260"
     CDS             6958..7650
                     /locus_tag="VFC01_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN81416.1"
                     /translation="MNFDWEGLAEGVVRRRLPFLDVSVGLIYSRDVAVLVDSGTTLTE
                     ARQIAQDVRDMAGCHVSHIVLTHNHFDHILGASAFDEAEIYCAPEVGVTMADGTDHLR
                     ADALRFGADADEVDRAIAALRVPEHQVSSAVLDLGDCTVTISHPGRGHTDHDLIAVVT
                     GADTRVVFCGDLVEESGDPSVGADSDLVAWPATLDRVVEAGGADALFVPGHGAVVDAA
                     FIRRQHDWLRRL"
     gene            7719..7949
                     /locus_tag="VFC01_17265"
     CDS             7719..7949
                     /locus_tag="VFC01_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6400 family protein"
                     /protein_id="HZN81417.1"
                     /translation="MRDVDFSYDLTLDEARRRAAVLEAIGDDWDPIAVLAEEEKAYDM
                     LYSDLDEEQQRIYDELVSAGVLPDRTVNRVAD"
     gene            complement(8181..8384)
                     /locus_tag="VFC01_17270"
     CDS             complement(8181..8384)
                     /locus_tag="VFC01_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81418.1"
                     /translation="MRRYQQGDKVTARRMIADMNVPEVPAGSVGTVVATTVLGKPKKV
                     HFALKTAWGPKQFHVDVRRGDVD"
     gene            complement(8679..9818)
                     /gene="pe"
                     /locus_tag="VFC01_17275"
     CDS             complement(8679..9818)
                     /gene="pe"
                     /locus_tag="VFC01_17275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase PE"
                     /protein_id="HZN81419.1"
                     /translation="MRRLFASVTALVTVGATGCFGTGVAAADDTQAGWTPDHGAATDG
                     PPLLGTPGRGYALGGAHVLGIPYDEYIKRTGADWFPGLDRQIVGYPAGQVQGHVLERL
                     FPGIGRLDDNFPGLGIDGPSIGESVDEGEGNLETAIRNGGPGTAIGLSEGAMVLDAVQ
                     AKLANDPTAPPPNTLSFATYGDPLAKHAFGESFLTQMFPVGSVVPALDFRMPPPVESQ
                     YDTYQFVSAYDSIADWPDRPDNWMSLLNAVVGLATGHTAVAFTNPSMVPPQNIATTVN
                     SKGAKTTTYLIPEQHLPLVLPFKYLGVDEGTLNQLDGVLKPMVDAGYSRNDNPLTAPI
                     TVDPVNGYDPAAVTAPATQAAFGGGTDPLSQLLAGAQYVLNHQQP"
     gene            9866..10060
                     /locus_tag="VFC01_17280"
                     /pseudo
     CDS             9866..10060
                     /locus_tag="VFC01_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880029.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10025..10138)
                     /locus_tag="VFC01_17285"
                     /pseudo
     CDS             complement(10025..10138)
                     /locus_tag="VFC01_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(10157..11569)
                     /locus_tag="VFC01_17290"
     CDS             complement(10157..11569)
                     /locus_tag="VFC01_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN81420.1"
                     /translation="MTTSIVVGAGPNGLAAAVALAKAGVQVTVLEAADEIGGGTRTSE
                     AIIPGLLHDHCSAIHPMAVGSRFLAGLGLERYGLSWRLPKIDCVHPLDGGSAGVLYRS
                     VDATADGLGADGARWRTLFGKTSANFDTLAEDIMGPLLHVPHHPLALARFGGPTVLPA
                     STLARWFRSPEARALFGGVAAHAFRPLHYPMTSAIGLGILTAGHRHGWPVATGGSQSI
                     TNALAALLSDLGGKIETGTRIETASQLPRADVTMFDLAPEAVAGILGDRLPSRVSRAY
                     RRFRQGPGAFKVDFAVEGGVPWTNPSAQAAGTVHVVGSYAELAATERDIHAGRMPARP
                     FVLVGQQYLADPQRSVGNVHPVWSYAHVPNGYSGDATEAIIAQIERFAPGFRDRIVGK
                     TVRTTTQMSTYNPNYVGGDIMTGSKDILQLTFGPRITLSPYQVGVPGMYICSAATPPG
                     PGAHGMCGANAAKVALSELN"
     gene            complement(11566..>12114)
                     /locus_tag="VFC01_17295"
     CDS             complement(11566..>12114)
                     /locus_tag="VFC01_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HZN81421.1"
                     /translation="WAFAPGAETKLNNVTRTAKNQVDQFIDFGYEPKWADVTMQRVIC
                     WRTMHSQGRGLFSVLLNSLPDIDTRSVREGEFVCNSLIGFNFGDGHLHNADMIAAVQR
                     EAGFEPGELIVVWVESQAWGSKVQHYKVIDAALGVIERGTWKVTDAVAEQPWLPNGPI
                     PTEVSWSLSGRKPADHDHGALA"
BASE COUNT         1974 a         3895 c         4044 g         2201 t
ORIGIN      
        1 tcaccacggt cagcggcggc agtatcaggt acagctacgc gccccgaccc taggacggct
       61 ctgaaaactc ttcggccacc gccgatgccg cctcgtcgat gatggcgcgc atggcgcgct
      121 cggcggcttc ctcgtcccgc atccggatcg cgcgggcgac ctcgtcgtgc agttcgatgg
      181 ccgccgggtt gggcaggtcg ggcatcatgc cgtggtgagt gcgcccggcg agcacctcgg
      241 cgacgacgcc gttcagcgcc cgaaacatct cgttgccact ggcttcgagc atcgtctgat
      301 gaaaaatctt gtccgccaac aagtaggcct cgagatcacc tgaacggcca tgcatcacca
      361 tgtccgatac cgcggccgcc atgatcctgc actgatgcgg gtcggcgcga cgcgcggcca
      421 gtgccgcagc ggccggttcg aacccgcgcc gcagctccga gagcgacacc agctgagcgg
      481 cgcggtcgcc ggcctcgagt cgccaccgga tcaaccgagg atcgaagaca ttccacttct
      541 cggccggttg aatcgtgatg cccactcggc ggcgcgatgc gaccatgccc atcgactcca
      601 gcacgcggat cgcctcacgg gcgacgctgc gggacaagcc gtgatgcgcg ctgaccccct
      661 cgagcgtgag cacctggccg gccgggtact ccccggacac gatcgaggcg cccagcgcgg
      721 tgagcaaatt gtcgtgcaag gcgccgacgt ttgcttcggt ggtcacacat acatcttgtc
      781 ataactttgc tgacgttatt aaaagcatat gattctgcca ctcagttgca atcgtatgac
      841 ttttgatgca tgctgtgtga cgtcaaatac aactgggtag gtggagggga tggcgtcacc
      901 aatcgtcgtc atgggagttt ctggctccgg aaagtcgacc gtcggcgcgg cattggcgca
      961 gcgccttcgg gtgcctttcg cggatgccga cgactttcat ccgcccgcca acatcgccaa
     1021 gatgacggcc ggtcaacccc ttgacgacga cgaccgccat ccgtggctgg acgcgatcgg
     1081 ggagtggctc gctgcgcatg gcgacggtgg ggtgatgagc tgctcggccc tcaagcgcaa
     1141 gtatcgcgac cagttgcgcc gacattgccc ggatgtggaa ttcctgcatc tgtccggcac
     1201 acccgaggtc atcggccgcc ggcaggcaag ccggcccgga cacttcatgc ccgcgtccct
     1261 gctggcatct cagttcgcca cgctcgagcc gctcgagcct gacgaacgcg gaatcgtcat
     1321 cgatgtcgat caggacatcg attccgtcgt tgacaactac gtcgcgctat ctgccacgcg
     1381 cacaaccgaa caggagaacc gatgaccccg gcactcacaa tattggcggc cgatactgag
     1441 ctgcctcagc ccatcgcggg cggtggccaa ttggtagcgg ccgctctggc tggcatcgcg
     1501 atcatcgtcg tgctgatcac ggtcaccaag ctgcacccgt tcctcgcgct gatattcggt
     1561 gcattgacag tcgggatcgt cgccggattc ccgtcgctcg ataccttcga cgacacactg
     1621 agctccttca gcgacggctt cggcaccacc gcggccgggg tgggcatcct gatcgcgctc
     1681 ggcgcgatgt tcgccaagct gctcgccgat tccggtggtg cagacgagat cgtcgacacg
     1741 atcgtcggac acgcgtcgcc gcgcacattg ccatgggcaa tggccttggt aggcgccatc
     1801 attggcctgc cgatgttctt cgagatcggc ctagtgctgt tgataccggt catctacttg
     1861 gtcgccaagc gatcgcagct ctcgctgatc acggtcggaa ttcccgcgct ggcagggctg
     1921 tccgccatgc acgggctggt gccgccgcat cccgggccgc tgaccgcgat cgacctgttg
     1981 ggcgcggatc tgggcatcac gctggccttg ggtgtactcg tcgcgatccc gacggtcatc
     2041 gtcgccggtc cgctgttcgg caagctggcc gggcgctggg tcgtcgtcga cgtgccggac
     2101 cggttcgagg cggacgattt cgcccggaac ggcggaggtg cggtgacgac cgccgccgtg
     2161 ggtgccgagg gagagggcac gacgacccaa accaccaccc gcgtcgagcg tcggcgtccc
     2221 agcttcggca tcacgttgtt ctccgtgttg ttgccggtcg ggctgatgat gggcaaggcg
     2281 ttggtcgaca tcttcatcga cgacgaaacc aacgcgctgc gccagatctt cgacatcgtg
     2341 ggccgcccgc tgatggcgct gttgatcgcg gtgatcgtcg ggatcttcac cctgggtcgc
     2401 ggcgccgcga tgacccggga tcagatcgtc aagtgcatcg agacttcgct gccgccggtt
     2461 gccggcatca tcctgatcgt cgctgccggc ggaggtttca agcaggtgct cgtcgacagc
     2521 ggcatcggca cactgctcgc ggactgggcc aagggcgcga acgtgtccat catcctgctg
     2581 gcctgggtgc tggccgtatt gatccggctc gcaacgggct ccgcgacggt ggccaccatc
     2641 accgcatcat cactgatgct cggtctggtg gaaggcttga gcaccggtga ggtctcgttg
     2701 gtcgtgctcg cggtcggcgc cggctcggtg ttcttctctc acgtcaacga cgccggattc
     2761 tggatggtca aggagttctt cggcatgacg gtcggccaga ccatcaagac gtggtcgcta
     2821 atggagaccg tgctgtcggt cgccggcctg gtgctggtgc tgctgctggg catcgtcata
     2881 tgacgatcgc ctagcgccga aacggcattc cagcaggctt gttctcgaac ttttcctgcc
     2941 ggaatgccgt ttcgcggcaa tcagcccttg atgacctggg tgccgcccgc gagttcgtcg
     3001 tgcttgccct gcttggtcgg gctgctgttg atggtcaccg cgatgacgat caccgcgatg
     3061 agcgttagca gaccgccgat gaacggaatg atgttgagca acgtccacac gttgcggatc
     3121 gcggcctgct gaacgtccgg cttgggtgcc ccgcccggac cgcgcacgct caggcccagc
     3181 agcttcttac ccggcgtcca cccctgggtg gactcgaagg ccaagaaata gaggaacatc
     3241 agcaggccgg agaacaggcc ggtgacccag tagttcgtca ccgagccgat cgccaaggcg
     3301 atcgcgaacg cgacgatgtt gacgatgatc ccgtcgatga accgcgcgat gaaccgcagg
     3361 aggagaccgc caggttcttg tccgccgggc ggcggatatc cgccgggttc gccgtactgt
     3421 ccgggctggg gttggaaatc gccgcttgtc atgcggcaaa tgtaccgctc acatgggcaa
     3481 tcgtgagcag tttgacgcgc gctgaactca agaacggctc attggcgcgc aacgcaattc
     3541 gggctagctg aagcggctgc ggcggtgctt gacctgggca cgggcggtgt ctgtcagctc
     3601 gccggcacgt tcgcgggcgg tgtcggccag ttctggcgcg cgctcccgcg ccacctcggc
     3661 ccattccgcg ccgcgctcgc gggccagttc ggcgatttct gcgccgcgtt cgcgggcggc
     3721 ctcggccagc acgggtgcgc gttcgcgggc gacttcggcc cactcggcgc cgcgctcacg
     3781 ggcgacgtcc gcgaactccg cgccccgttc gcgggcgacg tgggccaatt cgcggccgcg
     3841 ttctgcgccg acctgtagac catgtcccac tttctcggcc aacgcgctgt ctgcgactgc
     3901 gccaccggtg gccgcgccga cgggcagagc cgcggtgacg gcctcggaca ccttgtgggc
     3961 tgcgcgacgg ccgcgccagc cgagggaggg tttgccctcg gtgtcgacag cggcgatgat
     4021 cagcccgccg atcaggctga cgtcggtcaa gaacgcgcga cgttcgtcag ccttccgctg
     4081 cgggtctgtc tcactccaga atgtatgtcc gccaaggctt cccggcacca cagtcagcgc
     4141 gagggcggca gatgccacac gtggcagttt gccgctggcg aggagcaaac cgccaccgat
     4201 ctgcacggcc ccgttgatgc gggcgacctt ttcggcatcg gacgggacgt ttgtgcccac
     4261 ggggtccggc agcttactca gaccctcgag ggtggggcgg gccgcgtcgg ccgcaggttt
     4321 ggggctgcgc agagcatcga cgccgcggct gatgaagacc gctgacaaca tcgggcgcgc
     4381 aattcgtcgg atcaacatgg gccctgtgtt ccccaggtca gcgggacgca aaccctagta
     4441 gcggttgtct ttgtccttgc gtccccgtat cggcatcacg taccagaacg ccacgagcgc
     4501 cagcagcgtg cacgcgcccg agatccaagc gccggtcctg cccacgacgg tatcgaagat
     4561 gacgacagct actccggcga gcgcgatgcc gagtaggacc attccgacca tcgcgtagct
     4621 gtgcgcagcg gacaccaagg ttttgaggcg atgtcggcga aacagcagtc gatgcatgct
     4681 gaccggtgcg accagcaaca cagtggcgcc gatggaacac gcgacggtaa tcagatagac
     4741 ggtgcgcatg acgccgtcga gctgcgtgaa gcgttgctgg aacggcagcg tcagcaggaa
     4801 cccggtgagc aattggacgc cggtttgcgc gacccgcagc tcctgcagca ggctcgacca
     4861 attgcggtcc agccgttgcg cttccgtctc gtcgcgatcc gtgtcccact tctcttgggc
     4921 ttcgggatgc tcgatgtcat ccacacggca attctggcat caaacggcgt agcgcagcag
     4981 cgtgatgccc gatcggaacg acttcggcgg ttccaccaag gtcaggcgcg ctataccggt
     5041 tccgtcggga aagaggcgcc ggccgcggcc ctgcaccgcc ggatagacga acaggcggta
     5101 ttcgtcgacg acacctgcgg cgatcagtgt gtgcgccagg ctgatgctgc cggtcagcac
     5161 gatttccctg ccaggttgca ccttcagcgc ggtggtcagc gcgacggcgt cgccgttcag
     5221 cacggtcgag ttctcccact gcggatcgct catcgtcgtc gacaccacgt acttggccac
     5281 ccggttgaga tagtcggtca cgccggtggc gtcgtcggtc tgtttcggcc agtagccgcg
     5341 gaagtcctcg aaggtctgcc gcccgaccag cagcgcgtcg gcctgcgcgt cctggcgatg
     5401 cgactcctcg agcagatcct cgtcctgcag ctggggatcg aaccagtcgt cgagcatctc
     5461 gattgatccg tccagcgtga tgttctgggt gaccacgagt tttcgcatac atgtactgac
     5521 ctcatgggtc cggtgaactc atcgccggag tagcttcgca ggcgtgacac tcgttgcagg
     5581 gatcgactcg tccacccagt cctgcaaggt ggttgtctgc gatgccgata ccggtgagat
     5641 cgtgcggtcc gcgtcgtcac ctcatcccga cggcaccgag gtcgatccgc agctgtggtg
     5701 gtccgcgctg cagtccagca tcggcgccgc gggaggactc gacgacgtgg cggcggtgtc
     5761 ggtcggcgcc cagcagcacg gcatggtctg tctggacggc gccggcgcgg tggttcgaga
     5821 tgccttgctg tggaatgaca ctcgctccgg tgccgcggct gccgaactcg tcgacgaact
     5881 cggcggtccc gccgaatggg caaagcggat cggcgtggtg cccgtcgcgg ccatcacggc
     5941 gacaaagttg cggtggctcg ccgatcatga gcccgcgcac gcggatgcga cggcggccgt
     6001 ctgcctgccg catgactggc tgacgtggcg gctgagcgac tcgacggata tcaccacatt
     6061 gcacaccgac cgcagcgatg cgagcggcac cggatatttc tcgtcggagg ccgacagcta
     6121 ccagcccgat ctgctcgagc tggcgatgcg ggggcgacga cccgcgctgc ccaccgtgct
     6181 tggccccagg gaatcggcag ggcaggcatc aacgggggcg ctgctcgggc cgggggcggg
     6241 cgacaacgcg gcggcggcgc tgggccttgg cgcgggcacg ggtgactgcg tcgtttcgct
     6301 cggcacgtcc ggggtggtga gcgcggtggg cgaggccgca ccgcatgatc cggaggggat
     6361 cgtcgccggt ttcgccgatg ccaccggacg gcagcttccg ctggtgtgca cgctcaacgg
     6421 agctcccgtg ctggcggcag tgacgacgat gctcggcatc gacttcgatg agctggatcg
     6481 gttggcgttg tcggcgccgg ccggagcgga tggcctcacc ctggcgccgt atttcgaggg
     6541 cgaacgttca ccgaatcttc ctagcgccgc aggcgcgttg cacggcatga cgacacgtaa
     6601 cctgttgccc gccaacgttg ctcgcgccgc cgtggagggc ctgctggcgt cgatggcata
     6661 ctgcgtcgac aagatctcgg cacagggcat cgacgtgcaa cgcatcatcc tgatcggcgg
     6721 gggagcgcgc tcggaggcgg tccgccgtat cgcacccgcg gtcctcggcg caccggtgca
     6781 tgtaccgacg ccggcggagt acgtcgcgct aggggcggcc agacaggcag catggactct
     6841 gtcgcaacag gattcgccac cgtcgtggtc gttcggggtg acggcgtcct acagcgctga
     6901 cccgacgccg gaggtactcg agcagtaccg cggagcgcag cgtttgacac tggggcagtg
     6961 aacttcgact gggagggact tgcggagggc gtggtccggc gtcgtctgcc gtttctggat
     7021 gtcagcgtgg ggctcatcta tagccgcgac gtagccgtgt tggtcgacag cggcacgacg
     7081 ctgaccgaag cgcggcagat cgcccaagac gtgcgggaca tggctggttg ccatgtcagt
     7141 catatcgttc tgacgcacaa ccactttgac cacattctcg gagcatcggc tttcgatgaa
     7201 gccgagattt actgcgctcc cgaagttggc gtcacgatgg ctgatggcac agaccactta
     7261 cgtgccgacg cgctgcggtt cggggccgac gcagatgagg tggaccgggc gatcgccgcc
     7321 ctgcgcgtgc cggagcacca ggtcagctcc gcggtccttg atcttggcga ttgcaccgtg
     7381 acgatcagcc atccggggcg tggacacacc gaccacgact tgatcgccgt cgtcaccggc
     7441 gctgatacac gcgtggtgtt ctgcggcgac ctggtcgagg aatcgggcga cccgtccgtc
     7501 ggcgcggact ccgatctggt ggcgtggcca gcgacattag atcgagtggt cgaggccggg
     7561 ggagccgatg cgctgttcgt accgggtcat ggcgctgtgg tggatgcggc gttcatccgg
     7621 cggcagcatg actggctgcg cagattgtga tgaatttcgt ccgacaaacg tgacgtaggt
     7681 cattgccgag gctaactacc gccgggaacg atgtcgcgat gcgcgacgtg gacttctcct
     7741 acgacctcac gctcgacgag gcccgacggc gcgccgctgt gctggaggcg atcggcgacg
     7801 actgggatcc gatcgcggtg ctggccgagg aggagaaggc ctacgacatg ctgtactccg
     7861 acctcgacga ggagcagcag cggatctacg acgagctggt gagcgccggt gtactgccag
     7921 atcggacggt gaatcgtgtt gccgattgac ccgagtgctg acgcctcccg acgcgcatgg
     7981 ctgccatgcc cacagtgcgg ccatggcgtc aactgtggtg actgccaaag caccaggaat
     8041 tgcgcgacgc attggcagta cctactgagc aacaagggca cgttggtcta cctgcagtgc
     8101 ccttcctgcg gccatctgtg gtcgaccaat acgcgtgagc gcacgcgcgg ccagtccgac
     8161 gctgcctgac tcgttcgggg ttaatcgacg tcgccgcggc gaacgtcgac gtgaaactgt
     8221 ttggggcccc acgccgtttt cagcgcgaag tgaaccttct tgggttttcc cagcaccgtc
     8281 gtggcgacta ccgtgccgac cgagccggct ggaacttccg gcacattcat gtcggcgatc
     8341 atccggcgag ccgtcacctt gtcaccctgt tgataccgtc gcacaacttc ctcctcatga
     8401 agtcgacggg tccgaccttc tcgaagcggt ggcgcactga agatcttctt agcgagtcat
     8461 gttcaggcgc aagttgttta accgtgaagt caataattga gggccggtag cccgagggct
     8521 atgccgccga catttcacgc gcggttgcga gttggcgaac gcggagtggt ttgccgtcct
     8581 cagccgaatt cggctgcgac gtaatggttc tatcgatcgc acctagcgga gattgtgcgg
     8641 gctctggtag tggcaatcga ctctcgaaca ggcactgctc atggctgctg atgattcaac
     8701 acgtactggg cgccggcgag gagctgcgaa agcggatcgg tgccgccgcc gaaggccgcc
     8761 tgagtggccg gcgcggtgac ggccgccggg tcatagccat tcaccggatc gaccgtaatc
     8821 ggggcggtca gcggattgtc gtttcgcgaa tatcccgcgt ccaccatcgg cttgagcacc
     8881 ccgtcgagtt gattcagcgt gccctcatca accccgaggt atttgaaggg caagacaagg
     8941 ggaaggtgct gctcgggaat cagatacgtc gtcgtcttcg cacctttgga gttgacggtc
     9001 gtggcgatgt tctgcggcgg caccatgctc gggttggtga acgccaccgc ggtgtgaccg
     9061 gtcgccagcc ccacgaccgc gttgagaagg gacatccagt tgtccggcct gtctggccag
     9121 tcggcgatgc tgtcgtaggc cgagacgaat tggtaggtgt cgtactggct ctcgacgggg
     9181 ggtggcatgc ggaagtcgag cgcggggacg acgctgccga cgggaaacat ctgcgtcagg
     9241 aaactctcac cgaaggcgtg cttcgctaac gggtcgccgt aggtcgcgaa gctcaatgtg
     9301 ttcggcggcg gcgcggtcgg atcgttcgcg agcttggcct gcacggcgtc gagcaccata
     9361 gcgccctccg acaagccgat agcggtgccg ggaccaccgt tgcggatcgc cgtttccaga
     9421 ttgccttcgc cctcgtcgac cgactcgccg atgctggggc cgtcgatacc caggcccgga
     9481 aagttgtcat ccagccgccc gatacccgga aacaatcgtt ccagcacgtg accctggacc
     9541 tgtcctgcgg ggtagccgac gatctgccga tccagtcccg ggaaccagtc cgcacctgtg
     9601 cgcttgatgt actcgtcata gggaatgccg agaacgtgtg cgcccccgag cgcgtacccc
     9661 ctccccggcg tcccgagtag cggtggcccg tcggtcgctg ccccgtggtc tggcgtccag
     9721 ccggcttgcg tgtcgtcggc tgccgcgacc ccggtaccga aacatcctgt agcgccgacg
     9781 gttaccagag cggtgaccga tgcgaacagt ctccgcattc ccactcccgc ctgctcgact
     9841 acgcctgtac tgaaccggac cttacggtcg cgccgcgtcg ttccttgcgc cgtggttgtt
     9901 ttcggcgttc atcgacatat tcggcaccga ccgcgccggc atgggcgggc tgtgcgttgt
     9961 tctcgtggcg ggtcttgtcg cgatgctcgc ggtgcgaaca ccgggaaggt cgttggcgac
    10021 agcatcaggg aacggggcgc ggccactccg gacgcggtaa ttgggatcct ggcggccgta
    10081 atctgtctag caccacgcgc agtggtggcc aggtggcgcg gccgcggcga gtcactgccc
    10141 cgttctcggt tggcgttcag ttgagttctg aaagtgctac cttggcggcg tttgcgccac
    10201 acatgccgtg tgcgccgggg cccggcggtg tggccgccga gcagatgtac atgccgggga
    10261 ccccgacctg gtacggcgac aacgtgatcc gaggcccgaa cgtcagttgg aggatgtcct
    10321 tcgagccggt catgatgtct ccgccgacgt aattcgggtt gtaggttgac atttgggtgg
    10381 tcgtgcgcac cgttttcccg acgatccggt cccggaagcc cggggcgaac cgctcgatct
    10441 gggcgatgat cgcttccgtc gcgtcgcccg agtatccatt cggcacatgc gcgtacgacc
    10501 acaccggatg gacattgccg acggatcgct gcggatcggc gaggtactgc tggccgacca
    10561 atacgaatgg tcgcgcgggc atgcggccgg catggatgtc gcgctcggtt gccgcgagct
    10621 cggcatagct gccgacgacg tgaacggtgc cggccgcctg cgccgaagga ttggtccacg
    10681 ggacgccacc ctccaccgcg aagtccacct tgaatgcgcc cggaccctgc cggaatcgcc
    10741 ggtacgcgcg ggaaacgcgc gacggcaatc ggtcgccaag gattcccgcg acggcttcgg
    10801 gcgccaaatc gaacatcgtc acatcggcgc ggggcaactg cgaggccgtc tcgatccgcg
    10861 tacccgtttc gatcttgccg ccgaggtcgg acagcagcgc cgccagcgcg ttggtgatgg
    10921 actgcgaacc accggtcgcc accggccatc cgtgccgatg ccctgcggtg agaatgccca
    10981 acccgatcgc cgacgtcatc gggtagtgca gcggccgaaa ggcgtgggcc gccacaccac
    11041 cgaataacgc ccgcgcctcc gggctgcgaa accagcgggc aagagtggac gcgggcagca
    11101 cggtcggccc gccaaaccgg gccagcgcca gcggatgatg ggggacgtgc aacagcgggc
    11161 ccatgatgtc ctcggccagc gtatcgaagt tggccgacgt cttgccgaac agcgtccgcc
    11221 accgcgcgcc gtcggcgccc aacccgtctg cggtggcgtc gaccgagcgg tacagcacgc
    11281 ccgcgctgcc gccgtcgagc gggtgcacgc agtcgatctt ggggaggcgc cacgacagcc
    11341 cgtaacgctc caggccgagg ccggcgagaa atcgcgagcc gaccgccatc gggtggatcg
    11401 cggagcagtg gtcgtgcaac aggccgggga tgatcgcctc gctggtgcgg gtgccgccgc
    11461 cgatttcgtc ggccgcttcc agcacggtga cctggacgcc ggccttggcg agggcgaccg
    11521 cggcggccag gccgttcggc ccggccccca cgacgatgga ggttgtcatg ccagcgcgcc
    11581 gtgatcgtga tcggccggct tcctgcccga caacgaccag ctgacctcgg ttgggatcgg
    11641 gccgttgggc agccacggct gctcggcgac ggcgtcggtc actttccatg tgccgcgttc
    11701 gatcacgccg agcgccgcgt cgatgacctt gtagtgctga accttgctgc cccaggcttg
    11761 tgactcgacc cagacgacga tgagttcgcc tggctcgaaa cccgcttcgc gctgaacggc
    11821 cgcgatcatg tccgcattgt gcaggtgacc gtcgccgaag ttgaacccga tcaacgaatt
    11881 gcagacgaac tcgccctcgc gcacggagcg ggtatcgatg tccggtagcg agttcagcag
    11941 caccgagaac agtccgcggc cctggctgtg catcgtgcgc cagcaaatca cccgctgcat
    12001 cgtgacatcg gcccacttgg gctcgtagcc gaagtcgatg aactggtcga cctggttctt
    12061 ggcggtgcgc gtcacattgt tcagtttggt ctcggcgccc ggcgcgaatg ccca
//
LOCUS       DASRJA010000391        15027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2264473,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000391 DASRJA010000000
VERSION     DASRJA010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15027
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2264473"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VFC01_17300"
     CDS             complement(<1..720)
                     /locus_tag="VFC01_17300"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN81422.1"
                     /translation="MAAQIQAFLLGEQMPVVETTVDQRLWAGAGEAIRTVDGRRLHYE
                     ELRAATDHLAADLVAQGFQAGMPAGIVSSRTPEAIVAIFAVWKAGGTIVPIDAAWPAA
                     RVETALAAANVTCAFIDGNTAQHPGAWRLKEFEWSLTGLIKGAAADPAPAYVIFTSGS
                     TGVPKGVAVSHAALGNYLSWRAATLPLPPGFRVLAMASLGFDVMLREVVWPLTMGGTV
                     VLLPDAQRMDSSLVVAALREHQ"
     gene            1140..3233
                     /gene="pabB"
                     /locus_tag="VFC01_17305"
     CDS             1140..3233
                     /gene="pabB"
                     /locus_tag="VFC01_17305"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HZN81423.1"
                     /translation="MNHSSPRVLVVDNHDSYTYNLVHLIASITGQKPDVVTNDDLVVD
                     AALDGRYTHLVISPGPGHPAAVADYGNCLRLTREARIPLLGVCLGHQGLAVAFGGRVD
                     RIAPAHGVITHVRHDGHALFQHLPQGFRAVRYHSLAVLDVPDDLVVTARSDDGVIMGL
                     RHRSRELHGVQFHPESVLTDHGLRLMGNFLGGGRVAPRPRRPGRVKRSTSRRGQAMVS
                     RLDFWVEPEHLIAAAARRSTRVAWLDSSSVCDWSGRFSYLAWLEPDDLSITYDTHSRT
                     LTRHRAGTTTVTAGVDLFATLSDWLRAEVTGPAATPFGFTGGWVGYVGYECRAITTGG
                     PIRHSPTPDACLMRVTRYFAFDNATHAVYAVSLDPDAPARIESMRRFAVRSCGAASNL
                     STHVPGPAPGDSSSARPRYDKAFAQVQDELHAGNSYEVNLTYQSSLESTAAPLAVYSY
                     LRRVNPAPYSAFLCHAGVSVLCSSPERFLKIDAGGRLETKPIKGTMPRDPCPDRDAAN
                     RRSLSTDARFRSENLMIVDLLRNDLGVVCEIDSIEVPQLMAVESYASVHQLVTTIRGR
                     LAAGHDSVDALRAMFPPGSMTGAPKIRTTQIIETVEDSPRGVYSGVLGWLGFNGCADL
                     AVVIRTLVHHDGTYTFGVGGGVTVQSDPDSEYAETEWKSERLLTALRMAEKTSAEDPT
                     RAPATVGGIEAKGEA"
     gene            3302..3874
                     /gene="folE"
                     /locus_tag="VFC01_17310"
     CDS             3302..3874
                     /gene="folE"
                     /locus_tag="VFC01_17310"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZN81424.1"
                     /translation="MRDLGQAEDAVRDLLSALGVDVNDEHIQGTPRRVAVALAEFLTP
                     EPFVATTFSNDGQYDELILVRSIRFSSLCAHHLLPFVGVAHVGYLPGDRIVGLSKLAR
                     MVNYFASDLQLQERLTMQIAHWLQDAVGARGAGVVISAEHMCMSLRGARAHDAVTVTS
                     CLSGLVRTDPKTRAEFLALAREPTLGRPTQ"
     gene            3871..5184
                     /locus_tag="VFC01_17315"
     CDS             3871..5184
                     /locus_tag="VFC01_17315"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HZN81425.1"
                     /translation="MTGGTTTLRQSPAQERLWFRHALDPGDLGYHVLGAYELRGALDV
                     GALRRAVRGVVARHPALRARFPADEDPVQVIESDSRVILEEFTASSDLREHILAWLGK
                     PFDLEAGPLMRIGLWRLGPEDHILALCLHHIVCDHFSWLAILDELGEFYADADTRIGA
                     DERLLEYRQHIWAECQAAEEIGGPPQAELAGQLMPLHLPPPHPGESPEGHGHLDADLP
                     PGAVRMAESAARANGISPIGFHLGLTAGLAAFLCDTNDVVLTSPLTLRRPESSRSLVG
                     CFVNLALLRLAVDLDEPVVALTIRARNALVDAIGDRMRPYQAVVAQLRAARQISGADL
                     PFGLSYNYVADAERPPEFAGLTATAYPCPQLGLRNPFAVTVVADSLHTRIRLGWKRSL
                     MSSRAAGAFAETYQQALVRLSTPPRQSLREVLTGLPLTRLAYPTR"
     gene            complement(5169..5840)
                     /locus_tag="VFC01_17320"
     CDS             complement(5169..5840)
                     /locus_tag="VFC01_17320"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HZN81426.1"
                     /translation="MTGRPHVEVVLAMSVDGHIADGSRSPHRIGSRADREHLDRRLAD
                     CDAVLMGASTLRAIGYALQIRDPGLLAARPERHGSRQPVQIVASLSGKLSDSMAFFAE
                     PVPRWLVTVSSSAGAWQNRDHFDRVVTAGDDEVDWHDAMSQLAAGGIGRVCLAGGGDL
                     VAGVLGQGLVDEIWMTVCPVLIGGRGAPTPVDGPDLLLGQLPAVSLVTAEQAGEELFL
                     HYRVG"
     gene            complement(5837..9055)
                     /locus_tag="VFC01_17325"
     CDS             complement(5837..9055)
                     /locus_tag="VFC01_17325"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HZN81427.1"
                     /translation="MAQQSGPDTGSPVALDHGPNPPLSLAQERVWFYDVLAPEAPFHN
                     VPRLIAIRGPLQPELLARAMTRIVQRHHVLRCTYHLRNGVMVQQVNDPVPVTVPVLDL
                     SGLREAERETELDRISNELVAMPFDLQTGPLMRGHLIRMSADHHVFLLDFHHIACDGW
                     SERITLNEISALYGAAVAGDPEPLPELGLQYFDYAVWQRQRLASDAVRPHHEYWRGKL
                     GDEIPRLELPTDRPVPLRRRYEGASMGYELDPVLAQDLRRLARDHNCTLYMLVLTAFV
                     ILLHRYTGQDDIVIGSPVNNREREEFHDLIGYFVTRLPLRADLSGNPTVTEMLVRMRR
                     TVLEAFQYKDTTAERWPYPRADEDVRDSSNYQVMFFFQENPVGVERRFGGLVVTNANA
                     ASHQRVATMGLRSPTLGSQLDLGFFMEPVGERVFGWIEYATDIFDGSTVAAMRRDYIA
                     LLRCLADKPGSTIADLDFVDTGGQDRLAPPLPTAVDGYDDLVLPQLFADAAAAHPDRI
                     ALADGATALTYAALDARANALAHKLSGELCQERAVIGVVLGRSAGLPVSVLGVLRAGH
                     TVMCLDPALPDARVAHLLDLAGAVAVVAAPEEAERFAAYRYSVFHPPMAGSPDAPRHR
                     ISGSDLAFVFATSGTTGEPKLVAVEHRQAAVGQLPAHVPYPLAESDVLLLTSPPGSAR
                     LVGEVFWPLLAGSRVAVAPDGPIASAQWFTSVIGSGATVVSVIPPLLQTLIEELTDDQ
                     AAACPLRLLQVLGEPVPGWLARRVAERLPRAELINSYAQTEACPALFWSGRLAPGAAF
                     APARRESALSIAYVLDGHGRPVPYNVPGDIYIAGYTVARGYLNDKDLTAAAFLPDPRS
                     KRPGAHMFRTGDRGRIRRDGTLEVLGRMDNRVKVHGHSVAVEEVEAALTGESSVAEAM
                     VSVEPGPAGDNRLIALVVPLAGLTVNPTDLRTALAAKIPAYMIPVRIGMVDSLPRTPN
                     GKLDRTAVVALPAELPTRDLTPPRTDTERRILELWSAVLGTSQIDILDSFFELGGASL
                     DGIRLVARVSREFNVEMDLRLLVEQRTVAGMAARVDELVGAS"
     gene            complement(9073..10839)
                     /locus_tag="VFC01_17330"
     CDS             complement(9073..10839)
                     /locus_tag="VFC01_17330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HZN81428.1"
                     /translation="MSLPAPDVVACVRAFAETTPDAPAMHAPDRSLNFRELMAQADAL
                     AGAIRALAVAPDDCVVIAGVRTADTMVSLLASLVAGVPFMVIDDRSRPAVIESTLASV
                     SSRIAVAEGEFGRRLVALGYTVVPPSGHPPATSTPPEVAEDALAYVVLTSGTTGRPKQ
                     IAVERRHLNAYVQGLLDRLALTTPASFACVSPLWTDLGHTAIFGALCSGGRLLLASAE
                     TGTSALLLAKEFAAHPVDYLKITPSHLAALLSEGDAAVLPRTGVLLGGERLPWALCAK
                     VRALRPGIRLINHYGPAECTIGVAAGPVPAELPEGARTVPVGPPLAGTLFRTLSDGLT
                     PTSTGEVGELYVSGPTVARGYLGDPTQTAERFVMMDGVRWYRTGDLARPLPPDRFEIV
                     GRADRQVKVRGFRVEPGEVEHALLAGGLVAEAYAFAVTTGDTAAMAAAVVVRDGATAA
                     QVASDVAGRLAAAAVPAPVVLVEQLPRTASGKVDEAMLTALALKEFEPQSAVPAADLA
                     AALTSIEKDLVGVWAEILGRDVDTRTSVFQLGANSIAAIRFLARLQRQYDVEIPLHVV
                     FECPTIAELAALVDDALPAGRS"
     gene            complement(10869..11846)
                     /locus_tag="VFC01_17335"
     CDS             complement(10869..11846)
                     /locus_tag="VFC01_17335"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZN81429.1"
                     /translation="MATLTTQSASGATVVRPDAPETNLYEWTLGHAAQVDTLLREPGA
                     IIFRGFAMPSREDFEKICTEVSGPFMEYEYRSNNRQRLTGSLFNSTEYPASERIPFHN
                     EMSYFAVWPRHVWFLCEIPAAADGDTPLADSAEVYRQLHADIREPFERLGVRYTRTIG
                     PDVDLSWRDVFQTDDQRAVGEYCDRFEIEHEWIGDETVRTAQNRPAVVLHPHTGDRIW
                     FNQAHLFHPSSLGEDVHHWLLRRYGEDNLPRNAMFGDGSPIPLPALEAIRATYARLSM
                     PTNWESGDVLVFDNMRYAHARDRFTAPRRIVVGLTGAVDDSQIQLPASL"
     gene            complement(11856..12656)
                     /gene="folP"
                     /locus_tag="VFC01_17340"
     CDS             complement(11856..12656)
                     /gene="folP"
                     /locus_tag="VFC01_17340"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005197833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZN81430.1"
                     /translation="MQIMGILNVTPDSFSDGGEHLDPDAAVAWAEQMLSEGADIVDVG
                     GESTRPGAAPVDAQAEMHRVLPVVEALAQRCVVSIDTMKADVARAAVAAGAQIINDVS
                     ASMAHVAAELGVGWIAMHMQGAPRTMQTNPSYTDVVAEVRNFLGERAEAARALGVPRV
                     WIDPGIGFGKRYVDNLDLLRRLDVFVDTGIPVVLGASRKSFLGTLLGQPVVNQRVVGS
                     AMVAVYGEAAGVDIIRVHDVRATRDALLVARALNAPVGTPGPAAVNRA"
     gene            12770..14038
                     /locus_tag="VFC01_17345"
     CDS             12770..14038
                     /locus_tag="VFC01_17345"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN81431.1"
                     /translation="MTMTFDMVCVVLRSRLLTPLRSREFSQLIFGQFLSGVGDGAFLV
                     AVVTVMVVNRNSATDLGIVLGTRSAVALAAMFFGGVVADFARRTRIMAATDAVRGVLV
                     LVAAFTSPFAPLWVWVAIAATIGLASSAFQPAYQAAVPAFVPSDALEAGNALKVLSTR
                     GALILGPSIGALLVSTGGVRLAFLVDACTFMISLATLVGLKEPPHTTSAERRNPLRAA
                     AQGLRAVLDRPWIAWVIGSGTLQIMFVLAPMTIMIPLVLHARHQDAFYGLVVGFRSAG
                     SVIGTVAAAHWRPRRPGLAAMCGPLTMLILLAFFVIPAPAWAMLVAALLSGIGPSLFI
                     VYWPTALQRAVPDELRGRVFAFDQLGAYTLQPVGLVLVPLAVAALGFTWPTIIAAVVL
                     LVTTFVPLLIPGVTMFATPQQPAQKVTAPG"
     gene            complement(14566..>15027)
                     /locus_tag="VFC01_17350"
     CDS             complement(14566..>15027)
                     /locus_tag="VFC01_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZN81432.1"
                     /translation="EEGRDNDIVRETFERTGASVMGKRMFDAGEQAWPEEAPFHTPVF
                     VVTHEKRDPWERPGGTTFHFVNDGIESALDQAREAAGDRDVRIAGGGATILEYVNAGL
                     IDEFSIALSPVLFGSGIRLFEGVDAGRVALEPVRAEPTQRVTHLTYAVRQR"
BASE COUNT         2485 a         5084 c         4939 g         2519 t
ORIGIN      
        1 ttggtgctcc cgcagggccg cgaccaccag ggagctgtcc atccgttgcg cgtcgggcag
       61 gagcacaacg gtgccgccca tggtcagcgg ccagaccacc tcacggagca tgacgtcgaa
      121 tccgagcgag gccatggcca ggacgcggaa acccggcggc aacggcagcg tcgccgcccg
      181 ccacgaaagg taattgccaa gggcggcatg ggaaacggca acacccttgg gcacgccggt
      241 cgacccggac gtaaagatca cgtaggcggg cgcgggatcc gctgccgcgc ccttgatgag
      301 gccggtcaac gaccactcga attccttgag ccgccaggcg cctggatgct gggccgtgtt
      361 gccgtcgatg aaggcgcagg ttacgttcgc cgcggcgagg gccgtttcca ccctggccgc
      421 gggccaggcg gcgtcgatcg gtacgatggt gccgccggcc ttccacacgg cgaagatcgc
      481 cacgatggcc tcaggcgtgc ggctggagac gatcccggcc ggcatgccgg cctgaaaccc
      541 ttgtgcgacc agatcggcgg cgaggtgatc agtcgccgcc cgcagctcct cgtaatggag
      601 acgacgccca tcaaccgtcc gaatggcctc gcctgcgccc gcccacagcc gttggtcgac
      661 ggtcgtttcc acaaccggca tttgctcgcc gagtagaaat gcttggattt gcgccgccac
      721 cccgcgcctc ctgcctggtt tgtggagcgc atgtcctggt gttcttgcgg catcgctatc
      781 gccttgaggc gacacgaaat aacgcggccg actccacgcc cacgccccgt atctgcatca
      841 atccctgcga ggctaacgcg ggttacgaca aaagtaaatc gttggtatcg ttgggtgcct
      901 gtccacatga gaggagtcga aatggtacgt gtgatgaagc agttctgctt cgctgcctct
      961 acccggcgca tcaagaagac cggaaagtag ctttcccggc atggtgcgca cgtgctagac
     1021 gcgggaaggc gcgacgctgc ggttgcggcg tcgtgccttc gttgacaggc gagatacacg
     1081 caccgaaatt catctatgtc acgttgtgat gcgggactga tttcgaagtg tggaacaacg
     1141 tgaatcattc ctcgcccagg gttctggtgg tcgacaacca cgactcatac acgtacaacc
     1201 tggtccacct gatcgcgtcg atcaccggcc aaaagcccga tgtcgtcacc aatgacgacc
     1261 ttgtcgtcga cgccgcgctt gacggacgct acacgcatct tgtcatctcc ccgggtcccg
     1321 gacatccggc ggccgtcgcc gactatggca actgcctgcg gcttacccgt gaggcgcgga
     1381 ttcccttgct gggcgtatgt ctcggccacc aaggcctggc ggtggcgttc ggcggccgcg
     1441 tcgaccggat cgcgccggcc cacggggtga tcacccacgt ccgccacgac ggccacgcgc
     1501 tgtttcagca tctgccccaa ggatttcggg cggtcagata tcactcgctc gccgtccttg
     1561 acgtccccga tgacctggtg gtcacggccc gcagtgacga cggcgtcatc atgggactgc
     1621 ggcaccggtc acgcgaactg catggcgtgc agttccatcc ggaatcggtg ctgaccgatc
     1681 acggcctgcg gctgatgggc aacttcctcg gtggcgggcg agtcgcacca cgtcccaggc
     1741 gtcccgggcg tgtcaagcga tccacctcgc gccggggcca ggcgatggtt tcccgcttgg
     1801 atttctgggt cgagccagag catctcatcg cggcggcagc gcggcgctca acccgcgtgg
     1861 cctggctcga cagcagcagc gtatgcgact ggtccggccg attctcctat ttggcctggc
     1921 tcgagccgga cgacctcagc atcacctatg acacccacag ccgcacgctg accagacatc
     1981 gcgccggcac gacgaccgtc acggccggcg tggacctgtt tgccacgttg tcggattggc
     2041 tgcgcgccga agtcaccggt cctgccgcca cgccgttcgg attcacaggt ggctgggtcg
     2101 gatacgtcgg gtacgagtgc cgtgcgatca cgaccggcgg cccgatacgg cattcgccca
     2161 cgccggacgc ttgtctgatg cgggtcaccc gttacttcgc gtttgacaac gcgacacatg
     2221 ctgtctacgc ggtatccctc gatcccgacg caccggctcg gatcgagtcg atgcgtcgct
     2281 tcgccgtgcg gtcctgcggt gcggcttcca acctatccac tcacgtgccc ggcccggcac
     2341 ccggcgattc ctcatcggcc cggccgcgct atgacaaggc gttcgctcag gtgcaagacg
     2401 agctgcacgc cggcaactcg tacgaggtga acctcaccta ccagtcgtcg ctggagagca
     2461 ccgccgcgcc gctggccgtg tatagctacc tgcgccgggt caatccggcg ccgtatagcg
     2521 cttttctctg ccacgcgggc gtttccgtcc tgtgctcgtc gcccgagcgg tttctgaaga
     2581 tcgacgccgg gggtcgtctg gaaaccaagc ccatcaaggg aacgatgccc cgcgaccctt
     2641 gcccggacag ggacgccgcg aaccggcgct cgctgtcgac ggacgcaagg ttccgcagcg
     2701 aaaacctcat gatcgtcgac ttgctccgca acgatctcgg ggtggtgtgc gagatcgact
     2761 cgatcgaggt accccaattg atggcggtgg agagctatgc gagcgtgcac cagttggtga
     2821 ccaccatacg cggccgattg gccgccgggc acgacagcgt cgacgccctg cgggccatgt
     2881 tcccgcccgg atcgatgacc ggcgcgccga agatccgcac gactcagatc atcgaaacgg
     2941 ttgaggacag cccgcgcggt gtctactcgg gcgtcctggg ttggctgggc ttcaacggct
     3001 gcgccgacct tgcggtggtc atccgcacgc tggtccatca cgatggcaca tacacctttg
     3061 gagtcggggg cggcgtcacg gttcagtccg atcccgactc ggaatacgcc gagaccgagt
     3121 ggaagtcgga acgattgctg accgcgttgc gcatggccga aaagacgtca gccgaagatc
     3181 ccaccagagc acccgccacg gtgggcggca tagaggcaaa gggagaggca tgatcaacgg
     3241 atccgcttcg ggggccgcag ttggccgcac acacctgcga cttgcgccgc aaagggatcg
     3301 aatgcgcgat ctgggccagg ccgaggatgc agtgcgcgat ctactcagcg cgctgggcgt
     3361 cgacgtaaat gacgagcaca tccaggggac gccgcggcgc gtggccgtgg cgcttgccga
     3421 gttcttgacg ccggagccgt tcgtcgcgac gacgttctcc aacgacggcc aatatgacga
     3481 gctgattctg gtgcgctcca tcaggttcag cagtctgtgc gcccatcacc tgctgccgtt
     3541 cgtcggcgtc gcccatgtcg gttatcttcc aggcgatcgc attgtgggac tgtccaagct
     3601 cgcgcggatg gtcaactact tcgccagcga tctgcagttg caggaacgcc tcacgatgca
     3661 gatcgcccac tggcttcaag acgccgtcgg cgcgcgcggt gccggtgtgg tcatcagcgc
     3721 cgaacacatg tgcatgtcgc tgcggggtgc ccgggcgcat gacgctgtca ccgtgaccag
     3781 ctgcctgtcg ggcctggtcc gcactgatcc caaaactcgg gccgagtttc tcgcgttggc
     3841 tcgggagccg acgttaggtc ggcccacgca atgaccggcg gaaccacgac tttgcgccag
     3901 tcgccggcgc aggaaaggct ctggttccgg catgcgctcg accccggcga ccttggctac
     3961 cacgttctgg gcgcatatga gcttcgcggc gcgctggacg taggcgccct gcgccgagcg
     4021 gtgcgcggcg tcgttgcccg ccacccggcg ctgcgcgcgc gattccccgc cgacgaggac
     4081 ccggttcagg tgatcgaaag cgacagccgc gtcatcctag aggagttcac cgcgtcgagc
     4141 gatcttcgcg agcacatcct tgcgtggctg gggaaaccgt tcgacctcga agcgggtccg
     4201 ctcatgcgca tcggcctgtg gcgcctggga cctgaggacc acattctcgc gttgtgcctg
     4261 catcacatcg tctgtgatca cttcagttgg ctggcaatcc tggacgagct gggcgagttt
     4321 tacgccgatg cggatacccg gataggggcc gacgaacgac ttcttgagta ccgccaacac
     4381 atctgggccg aatgccaagc tgccgaggag atcggcggcc cgccgcaggc cgagttggcc
     4441 gggcagctga tgccgcttca cctgccgccc ccacaccccg gtgagagtcc agaaggccac
     4501 ggccacctcg atgcggacct tccgcccggc gccgtgcgca tggccgaatc ggccgcccgc
     4561 gcgaacggca tcagtcccat cgggttccac ctcgggctca ccgccgggct ggccgccttt
     4621 ctgtgcgata ccaacgatgt cgtgctcacc agcccgctta ccctgcgccg tcccgagtct
     4681 tcgcgatcgc tggtcggatg cttcgtcaac cttgccctgc ttcgcctggc cgtcgacctc
     4741 gacgaacccg ttgtggcact gacgatccgg gcccgaaatg cgctcgtgga cgcgatcggc
     4801 gatcggatgc ggccatacca agccgttgtg gcccaattgc gggcggcgag acagatctcc
     4861 ggagccgacc tgccctttgg cctttcctac aactacgtgg ccgacgccga acggccgccc
     4921 gaattcgcgg gactgaccgc caccgcctac ccctgtcccc agctcggcct gcgcaatccg
     4981 ttcgccgtca ccgtggtcgc cgactcgttg cacacgcgca tccggctcgg ctggaaacga
     5041 tcgctcatgt cgagccgtgc cgcgggcgcg tttgccgaga cctaccagca ggccttggta
     5101 cggctgtcga cgccgccgcg ccaaagcctg cgcgaggtgc tcacgggcct tcccctgacc
     5161 cgcctggcct atccgacccg gtagtgcagg aacagttcct cgccggcctg ttccgcggtc
     5221 accagcgaca cggcgggcaa ctgtcctaat aggaggtctg gaccgtcgac cggcgtcggg
     5281 gcgccgcgcc cgccgatcag taccgggcac accgtcatcc agatctcgtc gacgagtcct
     5341 tggccgagca cgccggccac cagatcacct ccgccggcca ggcagacgcg cccgatgcca
     5401 ccggccgcga gctggctcat cgcgtcatgc cagtccacct cgtcgtcgcc tgcggtcaca
     5461 acccggtcaa agtggtcgcg gttttgccac gcgcccgcgc tggatgacac cgtgaccagc
     5521 cagcgcggca cgggttcggc gaagaacgcc atcgagtcgg acagcttccc cgacaaggac
     5581 gcgacgatct gcaccggttg ccgcgagcca tgacgctccg gccgcgccgc aagcagccca
     5641 ggatcacgga tctgcagggc ataacctatg gcccgcaacg tggacgcgcc catgagcacc
     5701 gcgtcgcagt cggccaggcg ccggtcgagg tgttcgcgat cggcgcggct gccgatgcgg
     5761 tgagggctgc ggctgccgtc ggcgatgtgg ccatcgacgc tcatcgccaa caccacctcg
     5821 acgtgcggcc gaccggtcac gatgcaccaa ccagctcatc gactcgggct gccatgccgg
     5881 cgacggttcg ctgctcgacc agcagtcgca gatccatctc cacgttgaac tcgcgactga
     5941 cccgcgccac gagtcgaata ccgtcgaggg aggccccgcc cagctcgaaa aagctgtcca
     6001 ggatgtcgat ctgcgacgta cccaggaccg ccgaccacag ctcgaggatc cgccgctcgg
     6061 tgtcggtgcg cggtggggtc agatcgcggg tcggcagttc ggcgggcaag gcgacaacgg
     6121 cggttcggtc gagcttgccg ttgggcgtgc ggggaagcga gtcgaccatc ccgatccgca
     6181 ccgggatcat gtacgcggga atcttggccg cgagcgccgt acgcaggtcg gtcgggttca
     6241 cggtcagccc cgcaaggggt accacgaggg cgatcaaccg gttgtcccca gccggtccgg
     6301 gctcgaccga aaccatcgcc tcggccacgg acgattcacc ggtcagcgcc gcctcgacct
     6361 cctcgacggc gacgctgtgc ccatgcactt tcacgcgatt gtccatgcgg cccaagacct
     6421 ccagcgtccc gtcgcgccga atccggccgc ggtcgccagt gcggaacatg tgcgcccctg
     6481 gccttttcga gcgcgggtcg ggcaggaacg ccgccgcggt caagtccttg tcgttgaggt
     6541 aaccgcgggc cacggtgtag ccggcgatgt agatatcgcc cgggacgttg tagggcaccg
     6601 gccggccatg cccatcaagg acataggcga tcgacagagc ggactcacgc ctggccgggg
     6661 cgaatgccgc gcccggcgcc agccggccgg accagaacag ggcagggcag gcctcggttt
     6721 gcgcatagct gttgatcagc tcggctcgtg gcaaccgctc ggcgacacgt cgcgccagcc
     6781 agccggggac tggttcgccg agtacctgca gcaggcgcaa cgggcacgcc gcagcctgat
     6841 cgtcggtgag ttcctcgatc agggtctgca gcaacggcgg gatgacgctg acaaccgtcg
     6901 cgcccgagcc gatgacagag gtgaaccact gcgccgaagc gatcgggcca tccggcgcga
     6961 cggcaacccg ggagccggcc aacaggggcc aaaacacttc cccgacaaga cgagccgagc
     7021 ctggcggcga ggtcagcagg agcacatccg actcggcaag cgggtagggc acgtgggcgg
     7081 gcagctgccc gacggcggcc tgacgatgtt ccaccgcaac gagtttgggc tcgccggtcg
     7141 tgcccgaagt ggcaaacacg aaggccagat ccgagcccga tatccggtgg cgcggcgcat
     7201 ccgggctgcc cgccatcggt gggtggaaga ccgaataacg gtacgccgcg aagcgctccg
     7261 cctcctcagg tgcggccaca acggccacgg cgcccgccag gtcgagcagg tgcgcgacgc
     7321 gcgcgtccgg cagcgccggg tctaggcaca tcacagtgtg cccggcccgc aacacaccca
     7381 gcaccgaaac gggcaggcca gccgaccgtc ctaagacgac accgatcacc gctcgctctt
     7441 ggcaaagctc accagaaagc ttatgcgcca aggcattggc ccgcgcatcg agcgcggcat
     7501 aggtcagggc cgttgcccca tcggcgaggg cgatccggtc cgggtgcgcg gcggccgcgt
     7561 ccgcaaacaa ctgcggcaga accaggtcgt cgtagccgtc gaccgcggtc ggcagcggtg
     7621 gcgccagccg gtcctgcccg cccgtgtcca cgaaatccaa gtcggcgatc gtggaaccgg
     7681 gtttgtccgc gagacagcgc agcaacgcga tgtagtcgcg gcgcatcgcc gcgactgtgg
     7741 agccgtcgaa gatgtcggtg gcgtattcga tccagccgaa aaccctttct cccaccggtt
     7801 ccatgaagaa gccgaggtcc agctgggagc cgagcgtcgg ggatcgcagg cccatcgtcg
     7861 cgacgcgctg gtgcgacgcc gcgttggcgt tggtcacgac aaggccgccg aagcggcgct
     7921 cgacacccac agggttttcc tggaaaaaga acatcacctg gtagttgctg ctgtcccgga
     7981 cgtcctcgtc ggcgcgcgga tagggccagc gctcggccgt cgtgtccttg tactggaacg
     8041 cctccaggac ggtgcgacgc atccgcacga gcatctccgt caccgtcggg ttgccggaca
     8101 ggtcagcgcg caacggcaac cgggtcacga aatagccgat caggtcgtgg aattcctcgc
     8161 gctcgcggtt gttgacgggc gaaccgatca cgatgtcgtc ctgcccggtg tagcggtgca
     8221 gcaagatgac gaacgcggtc aataccagca tgtaaagcgt gcagttatgg tcacgagcca
     8281 atcgccgcaa gtcctgcgcc agcacgggat cgagttcgta gcccatgctc gcgccctcgt
     8341 agcggcggcg tagcgggact ggccggtcgg tgggcagctc caggcgcggg atttcgtcgc
     8401 caagcttgcc gcgccagtat tcgtggtgcg gacgcaccgc atcactggca agccgctgcc
     8461 gctgccacac agcgtaatcg aaatactgta ggccaagttc gggcagcggt tctggatcgc
     8521 cggccacagc tgcgccgtac aaggcgctga tctcgttcag agtgatccgc tccgaccagc
     8581 cgtcgcacgc aatgtggtga aagtcgagga ggaagacgtg atgatcggcg ctcatgcgga
     8641 tgaggtggcc ccgcatcaac ggcccggtct gcagatcgaa cggcatcgcg accagttcgt
     8701 tggagatccg gtccaactcc gtttcccgct cggcctccct gagcccgctg aggtccaaga
     8761 ccggcacggt caccggcact ggatcgttga cctgctgcac catcacacca ttgcgcagat
     8821 gataggtgca ccgaaggacg tgatgccgct gcacgatccg cgtcatcgcc cgtgccagca
     8881 actccggctg tagcggtccg cggatcgcga tgagccgcgg cacgttgtga aacggcgcct
     8941 cgggtgccag tacgtcatag aaccacaccc gttcctgagc gagggaaagt ggcgggtttg
     9001 gcccgtgatc cagcgcaacc ggagaaccgg tgtccggacc gctttgctga gccatgtgct
     9061 cctctccttt cgtcatgaac gcccggccgg cagcgcgtca tcaacgagcg cggcgagctc
     9121 ggcaatggtc gggcactcga agacgacatg gagggggatc tccacgtcgt actgacgctg
     9181 cagtctggcc agaaatctaa tcgccgcaat cgaattcgcc ccaagctgga acacgcttgt
     9241 ccgggtgtcc acatcgcggc ccaggatttc ggcccagaca ccgaccaggt ccttctcaat
     9301 cgaggtgagg gccgcggcca ggtcggccgc cgggacggct gattgaggct cgaattcctt
     9361 gagcgccaat gccgtgagca tcgcctcgtc caccttcccc gatgcggtcc gtggcaattg
     9421 ctcaaccagg acgacgggtg ccggcaccgc ggctgccgcc aggcggccag cgacgtccga
     9481 ggcgacctgt gctgccgtcg caccatcgcg taccacgacc gccgcggcca tcgccgcggt
     9541 atcaccggta gtcaccgcga acgcgtatgc ctcggcgacg agtcctccgg ccagtaacgc
     9601 gtgctcgacc tcgccgggct ccacccggaa cccgcgcacc ttgacctggc ggtcagcccg
     9661 cccgacgatt tcgaaccggt cgggcggcag cgggcgggcg agatcgcccg tgcggtacca
     9721 ccgcacaccg tccatcatca caaaccgctc cgcggtttgg gtcgggtcgc caaggtatcc
     9781 acgggcgacc gtcgggcccg acacgtacaa ctcacccacc tcgcctgtgg acgtcggggt
     9841 gagaccgtcc gaaagcgtcc ggaagagggt gccggccagt ggcggcccga ccggaaccgt
     9901 cctcgccccc tcgggtagtt cggcgggcac cggcccggcg gccacgccga tggtgcactc
     9961 ggccggtccg tagtggttga tcagccggat gcccgggcgc agcgcacgaa ccttggcgca
    10021 caaggcccag ggcagccgtt cgccgcccaa cagcaccccg gtgcgcggaa gcaccgcggc
    10081 gtcgccctca ctgagcaacg ccgcgagatg cgacggcgtg atcttcagat agtcgaccgg
    10141 atgggcggcg aattccttag ccagcaacaa agccgatgtg cccgtctcgg cgctggcgag
    10201 caggagccgg ccaccgctgc acagcgcacc aaagatcgcc gtatggccca ggtctgtcca
    10261 cagtggacta acgcaggcga agctcgctgg cgtcgtgagg gcgagccggt cgagcaggcc
    10321 ctggacgtag gcgttgaggt gccgacgctc gaccgcgatt tgcttgggcc ggccggtcgt
    10381 tccggacgtg agcacgacat aggccagggc gtcctcggcg acctcgggcg gcgttgacgt
    10441 tgcgggtggg tggccgctcg gcggcacgac ggtgtagccg agcgcgacga ggcggcggcc
    10501 gaattcgccc tcggcaacgg cgatgcgcga ggacaccgag gcgagcgtcg actcgatcac
    10561 ggcgggccgg ctgcgatcgt cgatcaccat gaacggcaca ccggccacga gcgaggcgag
    10621 gagggagacc atcgtgtccg cggtccgcac accggcaatg acgacacagt catcgggtgc
    10681 cacggcgagc gcgcgaatcg cgcccgccaa ggcatcagct tgggccatga gctcgcggaa
    10741 attgagtgaa cggtccggcg cgtgcatcgc gggcgcgtcc ggggtggttt cagcgaacgc
    10801 gcggacgcat gcgaccacgt cgggtgccgg gaggctcact atcgatgctc acttcctgga
    10861 ttcggcggtc aaagggacgc gggcaattgg atctgggagt cgtcgaccgc tccggtcagc
    10921 ccgacgacga tgcgccgcgg cgccgtgaac cgatcgcggg cgtgtgcgta ccgcatgtta
    10981 tcgaagacca gcacatcgcc ggattcccag ttggttggca tggagagccg cgcataggtg
    11041 gcgcggatgg cttccagcgc gggcaacgga atcggtgagc catcgccgaa catcgcgttg
    11101 cgcggcaggt tgtcctcccc gtagcggcgt aaaagccagt ggtgtacgtc ctcgccaagg
    11161 ctgctggggt ggaacaagtg cgcctggtta aaccagatcc ggtcgccggt gtggggatgc
    11221 agcacgaccg ccgggcggtt ctgggctgtg cgaacggttt cgtcaccgat ccactcgtgt
    11281 tcgatttcga aacggtcgca atattcgccc accgcccgct ggtcgtcggt ctggaagacg
    11341 tcacgccagc tcagatcgac gtcggggccg atcgtgcggg tatagcgcac accgagcctc
    11401 tcgaacggtt cccggatgtc cgcgtggagt tggcggtaaa cttccgcgga gtcggccaag
    11461 ggcgtgtcac cgtcggccgc ggcgggaatc tcgcagagaa accagacgtg ccttggccaa
    11521 accgcgaagt aggacatctc attgtgaaag gggatccgtt ccgatgccgg gtactcggtc
    11581 gagttgaaca ggcttccggt aaggcgctgc cgattgttgg accggtattc gtattccatg
    11641 aagggtcccg acacctcggt gcagatcttc tcgaagtctt cgcgtgaggg catggcgaag
    11701 ccgcggaaga taatggcacc cggctcccgc agcaatgtgt ccacctgtgc ggcatggccg
    11761 agggtccact cgtacaggtt ggtttccggc gcgtccggcc gcaccaccgt cgcgccgctc
    11821 gcgctttgcg tcgtcaatgt cgccatccct ttcgtttagg cccggttgac cgccgccggg
    11881 ccgggtgtcc cgacaggcgc gttgagcgcg cgggcgacga gcagcgcgtc acgggtggca
    11941 cgcacatcgt ggacccggat gatgtccacg cccgccgcct cgccgtagac cgcgaccatc
    12001 gccgaaccta cgacccgctg attgaccacg ggctggccaa gcagagtacc cagaaaagac
    12061 ttacggctcg cgccaagcac caccgggatg ccggtgtcca cgaagacgtc gagccggcgc
    12121 agcaagtcaa ggttgtcgac gtagcgctta ccgaacccga tgcccggatc gatccacact
    12181 cggggaaccc cgagcgcccg cgcggcttcg gcccgctcgc cgaggaaatt acggacctca
    12241 gccaccacgt ctgtgtagga cggattggtc tgcatggtgc gcggcgcccc ttgcatgtgc
    12301 atggcgatcc agccgacgcc gagctcggcg gcgacgtgag ccatcgatgc agaaacatcg
    12361 ttgatgatct gggcgcctgc ggcgacggcg gccctggcaa catcggcttt catggtgtcg
    12421 atcgagacca cgcatcgctg cgccagcgcc tcgacgaccg ggagcacacg gtgcatctcg
    12481 gcctgcgcgt cgacaggcgc cgcacccgga cgcgttgact caccgccgac gtcgactatg
    12541 tctgcaccct cggacaacat ctgctcggcc caggcaaccg cggcatcggg gtcgagatgc
    12601 tcgccgccat cggagaagga gtcgggcgtt acattgagga ttcccattat ttgcatgtga
    12661 tcaccccgcc agcgctccga gcgtgagggt gcatcgccat gccggccact ataaccaact
    12721 ctgtccgtgc tcgccgtcct cgatttcctc cacactgcac agtcacacga tgacgatgac
    12781 ctttgatatg gtctgcgtcg tgctgaggtc acgattgctg acgccgctgc gttcgcggga
    12841 gttttcccag ctcatctttg gccagttcct gagcggagtt ggcgacgggg cattcctcgt
    12901 cgctgtcgtc acagtcatgg tggtcaatcg aaactcggcc accgatctcg ggattgtgct
    12961 cggcacccgc agcgcggtcg cccttgccgc gatgttcttc ggtggcgtcg tcgctgactt
    13021 cgcccggcgc acccggatca tggcggcgac cgacgccgtg cgcggcgtgc tagttctggt
    13081 ggcggcgttc acctcgccgt ttgcgccgtt gtgggtgtgg gtcgcgatcg cggccacgat
    13141 aggcctcgcc tccagcgcgt tccaacccgc ctaccaggcc gcggtgccgg cgtttgtccc
    13201 aagtgacgca ctagaggctg gcaacgcgct gaaggtcctg tcgacgcgcg gcgcactgat
    13261 cctcggcccc agcatcggcg cgctgctcgt ctccaccggc ggcgttcgcc tggcgttctt
    13321 ggtcgacgca tgcacattca tgatcagcct cgccacgctc gtcggcctca aggaaccgcc
    13381 gcacaccacg tcggcagagc gccgaaatcc cttgcgggcg gccgcacagg gcctgcgcgc
    13441 cgttctcgat cgcccgtgga tcgcgtgggt catcggctcc ggcacattac agatcatgtt
    13501 cgttctagcg cctatgacaa tcatgattcc cttggtactg cacgcccgtc accaagacgc
    13561 gttctacgga ctggtcgtcg gctttcgctc ggctggctcg gtcatcggca cggtggcggc
    13621 ggctcactgg cggccacgcc gtcctggact ggcggccatg tgcggcccgc tgacgatgct
    13681 catcctgctg gccttcttcg tcattcccgc accggcgtgg gcgatgctcg tcgccgcact
    13741 cctgtccggc atcgggccgt ccctgttcat tgtctactgg ccgaccgcgc tgcaacgcgc
    13801 cgtgccggac gagctgcggg gccgagtctt cgccttcgac caactgggcg cctacacgct
    13861 gcaacccgtc gggctggtgc tggtccccct tgcggtggcg gcgctgggct tcacctggcc
    13921 cacgatcatt gcagcggtgg ttctgctcgt cacaacgttt gtgcccctgc tgatcccggg
    13981 cgtgaccatg ttcgcgacac cgcaacaacc ggcgcagaag gtgaccgctc ccggatgacc
    14041 gtgccgtgtt gtcgaccact gcggcaggtt gcaccggatt gcccatcagt ccatttgcga
    14101 cggcgacacc acgtctcgac tcccggtggg gagtgggcat ggaacgccgc tgttgcgttg
    14161 tcccacgccg tgccggttgt gttgctacgc aaagcctgtc tcgccaggag gccaggcgaa
    14221 gagtgtcgga ccgcctctcg gtcccggttc gcatctcgac agtgaggcgg gctctggcga
    14281 cgcgtgcggc cggtggtggc tttggagagg cggtggtgtc gcggcgttgg gcgcaggtcg
    14341 tctgaagggt ggtggcgtcg cgatcggtga gctggttggc ggcgggcagc agcgtctgct
    14401 gcatgaggtc ggtggcctgg cggtagcgct cgagtgccgc tggcccgtgt cccgggcgat
    14461 ggccagcgcg gtccgcggcc cgaacgctcg cccttcctga cgcttagagc gtcgaggatc
    14521 tcaccgcgct gctgacctcg gcggggagcg ctgaggtcga gacagttacc gctgccggac
    14581 tgcgtaggtc aggtgggtca cccgctgggt cggctccgcg cggaccggct ccagggccac
    14641 gcggccggcg tccacgccct cgaacaggcg gattccggag ccgaacagca cgggtgagag
    14701 cgcgatcgag aactcgtcga tcaggccggc gttcacgtac tccaggatcg tcgcgccgcc
    14761 gcccgcgatg cggacgtcgc ggtcgccggc ggcctcgcgg gcctggtcga gcgcggactc
    14821 gatgccgtcg ttgacgaagt ggaaggtggt cccacccggc cgctcccagg ggtcacgctt
    14881 ctcgtgcgtc acgacgaaga ccggcgtgtg gaacggcgcc tcctccggcc acgcctgctc
    14941 gccggcgtcg aacatgcgct tgcccatcac gctcgcgccg gtgcgctcga acgtctcccg
    15001 cacgatgtcg ttgtcgcgcc cttcctc
//
LOCUS       DASRJA010000392         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2319406,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000392 DASRJA010000000
VERSION     DASRJA010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2319406"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFC01_17355"
     CDS             complement(<1..201)
                     /locus_tag="VFC01_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81433.1"
                     /translation="MNPGRAVLVTGATGLIGAEVVARLSPRRPVIAVTHRQPQPIRTD
                     GSTVLSGEYGQSWTGRGVARLAA"
     gene            complement(198..473)
                     /locus_tag="VFC01_17360"
     CDS             complement(198..473)
                     /locus_tag="VFC01_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZN81434.1"
                     /translation="MSAPQQFPEPERQRVIRLITALAVPRRMPDDVADGARLIDDLGF
                     DSVRLIELTMALERAFGLPPQRPENLATVATVDDVVAFALTALKDPL"
     gene            complement(470..2212)
                     /locus_tag="VFC01_17365"
     CDS             complement(470..2212)
                     /locus_tag="VFC01_17365"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN81435.1"
                     /translation="MAIDWIDAPLADAGVFVADDVDGWHYRSYTELARESRRIGGALR
                     ANGLRVGDNACIVMPSGHRCVAAMYAVWLCGATVTQIAPPTFGDDAAYIERTSAILRS
                     ANPRSVLTTPELWHTVERAVRVAGLPREPLSLSEDLINAADPIGLTHGRAECALLQFT
                     SGSTGTPRGVMVSWANLAANLQSISRLLGWRSGDAMVSWLPLYHDMGLITLLMGIANQ
                     GSLYLMRPDQFIRDPMRWIRAMSGRQHSVCPSFGIDYAARRLSPEDIEGVDLTSWKSL
                     ATGAECIDLTALHAFSRLVEPAGFNPTALIAAYGLAEATLLVTGTPFDEPISAVRIDE
                     SSLRPGQPVAVLDCARFTGQQLQGTGWIAGLGPAGSEVSIIDQNGALLPDGALGEIAV
                     SSPSVAMGYHGDKSDPGTSTRFADAGVLHTGDAGFCYTGQVFVLGRMGSALKVRGKSV
                     FMEDLETRLAAATGIPKCKFCAVAHSGVGLPGVALFVEASAGSWVDTARRLLRSDLGP
                     APTIQIVIGPRRFIRRTSSGKPCRQRMWALLVAGDLVEGTVIDSSTITADREQTDAQA
                     ATCGGHQTHVRGVE"
BASE COUNT          441 a          791 c          775 g          444 t
ORIGIN      
        1 ggccgccagg cgagcgacgc ccctacctgt ccacgactgc ccgtactcgc ccgagagcac
       61 agtcgagcca tccgtgcgta ttggttgcgg ctgccggtgt gtcaccgcga taacaggacg
      121 acgcggcgac aaccgcgcca ctacctcggc tccgatcagc cccgtcgccc cggtgaccag
      181 cacggcgcgg cccggattca cagcggatcc ttcaatgcgg tcagcgcaaa ggccaccacg
      241 tcgtcgacag tggcgaccgt cgcgagattc tcgggacgct ggggcggaag gccaaaggcg
      301 cgttccaatg ccatcgtcaa ttcgatcagc cgcacggagt cgaatccgag atcatcgatg
      361 agccgggcgc cgtccgctac gtcgtcgggc attcgccgcg gcaccgccag cgcggtgatc
      421 agccggatca cccgctggcg ctcaggctct ggaaattgtt gcggcgcgct cattcgacac
      481 cccgcacatg ggtttgatgc ccgccacaag tcgctgcctg tgcgtcggtt tgttcccggt
      541 cggcggtgat tgtcgagctg tcgatcaccg ttccctcgac caaatcaccg gccacgagca
      601 gagcccacat cctttggcgg cacggcttgc cgctggaggt ccttcggatg aatctccgcg
      661 gcccgatgac gatttgaatc gtcggggcag gaccaagatc ggatcgcagt agacggcggg
      721 cggtatcgac ccagctgccc gccgaggctt cgacgaagag ggcgacgccc ggcagtccca
      781 ccccgctgtg tgcgaccgcg cagaacttgc atttgggaat cccggtggcc gcagcgagcc
      841 gtgtttcgag gtcttccatg aacacggatt tgccccggac cttgagagcc gaacccatcc
      901 ggccgaggac gaatacctgt ccggtgtaac agaatcccgc atcaccggtg tgcagcacac
      961 cggcatcggc gaatcgagtg gaggtgcccg ggtccgattt gtctccgtga taccccatgg
     1021 ctacggatgg cgaagacacg gcgatctccc cgagggctcc gtcgggcagc agcgcgccgt
     1081 tctggtcgat gatgctgacc tcggagccgg ccggacccaa accggcgatc cacccggtac
     1141 cttgtagctg ctgtccggtg aatcgtgcac agtcgaggac cgcaaccggc tgcccgggcc
     1201 gcagtgatga ctcgtctatg cgaacggcgc tgatcggttc gtcaaaaggg gttccggtga
     1261 ccagcagtgt ggcctcggcc aggccgtagg cggcgatcag ggccgtgggg ttgaaccccg
     1321 ccggttcgac cagtcgggag aatgcgtgca aggcggtcag gtcgatgcac tcggctccgg
     1381 tggccagtga cttccaggag gtaaggtcga ctccttcaat atcttccggg ctcagccggc
     1441 gagcggcata gtcgatcccg aacgacggac ataccgaatg ctgccgtccg gacatcgcac
     1501 gaatccaccg catcgggtcc ctgatgaact ggtcggggcg catcaggtag agcgacccct
     1561 ggttcgcgat tcccatgagg agagtgatca gtcccatgtc gtggtagagg ggcagccacg
     1621 acaccatggc gtcacccgat cgccagccga gcaacctgct gatcgattgc aggttcgcgg
     1681 caaggttggc ccagctgacc atcaccccgc gtggggtgcc ggtcgatccc gaagtgaact
     1741 gcagcagtgc gcattcggcg cgtccatgtg tcagacctat gggatcggct gcgttgatca
     1801 gatcctccga cagcgacagc ggctcgcgcg gcagacctgc gacgcggacc gctctttcga
     1861 ctgtgtgcca cagttcgggt gtggtcagca cgctacgtgg attggcgctc cgcagtatcg
     1921 cgctggtccg ctcgatatat gcggcatcat caccgaacgt cggaggcgcg atctgcgtta
     1981 ccgtggctcc gcacaaccag accgcgtaca ttgctgcgac gcatcgatgc cccgagggca
     2041 tcacgatgca ggcgttatcc cccacgcgca aaccgttcgc gcgcagcgcc ccgccgatcc
     2101 ggcgcgattc tctggcgagt tcggtgtagg aacggtagtg ccatccgtcg acgtcatctg
     2161 caacaaacac tccggcgtca gccaagggcg catcgatcca atcgatcgcc atagtggaag
     2221 tggaatccga cgtttgcaac aatgcttgcc tcaacaggct ttcgtccgtt atgcgcggtc
     2281 gcgtcgggtg ccgatgtcgc ctacgagatg cgcgattacg gcgttgacgt agtcggccgt
     2341 tcgagctcgg agacttctct gcaacgtgga tgaagacgtc cggctcgttc tcctggtacg
     2401 tctcctggaa gtcgccgtac ggactgaaag tcgacattga gctctccttc g
//
LOCUS       DASRJA010000393         8367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_895824,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000393 DASRJA010000000
VERSION     DASRJA010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895824"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VFC01_17370"
     CDS             complement(<1..723)
                     /locus_tag="VFC01_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HZN81436.1"
                     /translation="MTSQQPIGPVDATRVPRYAGPPTFARLPRTDEVTHTDVAVVGVP
                     FDSGVSYRPGARFGPGHIRAASKLLRTYHPRLDVEPFAVQQVADAGDIAVNPFDIAEA
                     ITTIERASDELRDGGTKLVTLGGDHTIALPLLRSLHRDHGPIAVLHFDAHLDTWDTYF
                     GAPFTHGTPFRRASEEALLDPEHCLHVGIRGPLYAPTDLSDDRVLGFQVIGTDDFQID
                     GLPAVIERMLARIGDAPVYVSVD"
     gene            806..2305
                     /locus_tag="VFC01_17375"
     CDS             806..2305
                     /locus_tag="VFC01_17375"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HZN81437.1"
                     /translation="MLTVAELVADPELGLELVAGRGGADREIEAAAVSELANPGPWLQ
                     GGELLLTIGLLLPQTTDGCRTYLTELDAAGVQAVGLGLGAELPHQRAPARLITAADEI
                     GMPLLTVPDPVPFIAVTKAVFAYRARAERHELEWALETQRALTAAAVTPGGLLGILAA
                     HRDATGRSGVVIDLLGRVLAESDHGGEGLVSRLSGLLESIRDQGLSAAAVDISDGRRR
                     ELHALGARRLRAWLLIDGPAELPAAHQISGDLVSLLSLELERRHGLNTAQRRGRAQVL
                     DRLARATVDDVVAARWLTSVGLPDADLRAAAVAAPDDADDLAADLLITVPDALVRVVG
                     EVVELAVPLDTDLPGVLGTLARSRPAGIGIAVRPGALVVSLRQARSALPASRVQGRHM
                     HAEEIASSRLLLNTVGAQPLQAFADAVLGPLDATDRSGELLRGLTAFLEHNGHWGSAA
                     NALRVHRHTMRNRIDAVERLTGRRMDSAQDRHELWLALRARDAARMSSD"
     gene            2335..3657
                     /locus_tag="VFC01_17380"
     CDS             2335..3657
                     /locus_tag="VFC01_17380"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN81438.1"
                     /translation="MRRYAYSVVVKGRPVCVPEAALESRVPARTRLHAETVARHLRVL
                     TVTTRIGAAVSIFFGVQGLIVGQDVVWIGMVNLASGVIFLLMPLLYRFGELVAPLVFY
                     FIAYASITVVCWHLGTGSGLPFYYLVAATLMVMILGIDHMVLASVLAALGAATVITLE
                     IVVPYNTGVQPDWAFRSGFILSVISAWLMVIAVVWYALRETRRARLAMEAEYERSERL
                     LTNILPATIAQRLKDPSRNIIADKYDDASILFADIAGYTKRASDTTPSDLVRFLDRLY
                     TDLDGLVDRHGLEKVKTSGDSYMVVSGVPEPRDDHIEALACLALDMADAVSDLKDPQG
                     REVPLRIGLAAGPVVAGVVGARKFFYDVWGDAVNVAARMETTDVEGRIQVPQDVYERL
                     NHAFLLEERGNVDVKGKGVMHTWYLVGRRNDEAVRGAVERDVAATGSS"
     gene            complement(3654..4463)
                     /locus_tag="VFC01_17385"
     CDS             complement(3654..4463)
                     /locus_tag="VFC01_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN81439.1"
                     /translation="MNLVHIEAGEGEPVLLLHGYPQSASCWRHQIPALATHHRVIAAD
                     WPGFGRADPPDTPATYDAEVERIGTLADSFGLNRFNLVAHDYGGFLGLGYVQRHPERV
                     LRLALLNTRAHNIFRPWFYHFSQGQRWLARHAPALLWRLPLRRLHHDALRPYRAMGCF
                     DERLEDEYLGWMDTPPGRQTFVDFFTNYHVPAVPGLAAGLGQIDCPTAIIWGDRDRYI
                     PFSTARELAERIPGATLTQLTGGDHYIMEERPDEVTAALLELLARKPSVLR"
     gene            4493..5110
                     /locus_tag="VFC01_17390"
     CDS             4493..5110
                     /locus_tag="VFC01_17390"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN81440.1"
                     /translation="MTRRRGRPRAYDPDVAMRQARDIFWRGGFAATSLDDLATGMSMN
                     RPSIYAAFGDKRALYLRAVTEYAEMSSGWLATALARPRSLRDGLHAVYRYARDFYQAG
                     DDAPRGCFLLGTAVTEANGDPHVRAIVEQTMSAFTATFAERFERAEREGELSPHAPDA
                     LAQIATAALNTLAVRARTGAPAAVLDALIDATVDVICARPSGSPT"
     gene            complement(5085..5576)
                     /locus_tag="VFC01_17395"
     CDS             complement(5085..5576)
                     /locus_tag="VFC01_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HZN81441.1"
                     /translation="MGTTTRRTETEVQGFQASPELSANLQKVLIDLIELHLQGKQAHW
                     NVVGTNFRDLHLQLDELVDFAREASDTIAERMRALDAVPDGRSDTVAANTTLPQFPAY
                     EHNTTEVVDLIAGRTYAAVDTLRTVHDAVDAEDPSTADILHELIDGLEKLAWLIKSEN
                     RKV"
     gene            complement(5606..7405)
                     /locus_tag="VFC01_17400"
     CDS             complement(5606..7405)
                     /locus_tag="VFC01_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HZN81442.1"
                     /translation="MTEPPHRAVISRLSADFAAMSQQLARMSADLIELDRLLSAQPAP
                     VAPPQPAPYVPPAVPYWPQYPPQYAHYPSPPPQVPPPPAPAKQPRQERSEGWIGKVLA
                     VAGVAVTLVGVVLLLVLAAQAGILRPEFRVAGGVVLAAALIAAAWWLYDRPGGRVGAI
                     ALAATAIAAAYMDVIAVTTIYHWVPAPVGLGIAAVVGGGGLTLARRWDSQHLGVLVLV
                     PLILLAPVVTDGITLLLVGFMLALSAASLPVQLGKDWIWLHAARIAAPTFPILVALAA
                     VYFDDHHDLWLAGACGIAAVLAIGAALILLPRTANRAVMALLTAAGVVPVLCVSLAVD
                     RVVAALMAAALAAALLAIVLLGPPNVTGVVRQIWSALSAIAALIAVTVAFDGYVAGPV
                     LLAMAVMVAIAGRRDDIARWAAIGFAVVGGGIYLSYAPPSSLMSATELDTPVAVSTLV
                     SSVLLVVCAITIAWSWKTVREQDRAAIWAGSAAVIAYVVTAFTVTAGVLIGGPNGGFF
                     AGHMAATICWIAMAAALFIYAARLAKAERSLPIGGGLALVAAAMAKLFLFDLGTLDGI
                     FRVVVFIVVGLVLLGMGAGYARLLEKQDLANLE"
     gene            complement(7450..8109)
                     /locus_tag="VFC01_17405"
     CDS             complement(7450..8109)
                     /locus_tag="VFC01_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN81443.1"
                     /translation="MGADGTPTVMVVDDHPIWRDAVARDLAEDGFSVVATADGVAAAR
                     RRAAVVHPDVVVMDMRLSDGDGAQATAEVLAVSPSSRILVLSASDERDDVLEAVKAGA
                     TGYLVKSASKQELGDAVRATAEGRAVFTPGLAGLVLGEYRRIAQRPGGGPDMPSLTER
                     ETEILRHVAKGLTAKQIAARLSLSHRTVENHVQATFRKLQVANRVELARYAIEHGLDK
                     E"
     gene            complement(8082..>8367)
                     /locus_tag="VFC01_17410"
     CDS             complement(8082..>8367)
                     /locus_tag="VFC01_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN81444.1"
                     /translation="LCAAAANALDNVASHAGPAARAYVLLEDLGDSVTVSIRDDGVGI
                     AEGRLEEAVAEGRVGIAKSIVGRMNWLGGSANLNTGPECGTEWELTVPRR"
BASE COUNT         1358 a         2785 c         2890 g         1334 t
ORIGIN      
        1 gtcgacggag acgtagaccg gggcgtcgcc gatgcgggcc agcatccgct cgatcaccgc
       61 aggcagtccg tcgatttgga aatcgtctgt gccgatcacc tgaaaaccca gcacgcggtc
      121 atcggacagg tcggtcggtg cgtacagcgg gccgcggatc ccgacgtgca ggcagtgctc
      181 cgggtcgagc agggcttcct cgctggcccg ccggaacggg gtgccatgcg tgaacggcgc
      241 gccgaagtag gtgtcccagg tgtcgaggtg agcgtcgaaa tgcagcactg cgatcgggcc
      301 gtggtcgcgg tgcagcgagc gcagcagcgg caatgcgatg gtgtggtcac cgcccagtgt
      361 gacaagcttc gtgccgccgt cgcgcaattc gtcggacgct cgctcgatgg tggtgatcgc
      421 ctcggcgatg tcgaacgggt tgaccgcgat gtcaccggcg tccgcgacct gctggaccgc
      481 gaacggctcg acgtccagcc gcgggtgata ggtccgtagc agcttggatg cggctcggat
      541 gtgccccggg ccaaatcggg ctcccggtcg gtaggagacg ccggagtcga acgggacgcc
      601 gacgaccgcg acgtcggtgt gggtgacctc gtcggtgcgg ggtaggcggg cgaaggtggg
      661 cggacccgcg tagcgcggca cgcgggtggc atcgaccggt ccgatcggtt gctgagacgt
      721 catgccgaga aggtttccgg aaatccgggc ctgcggatat agccattttg tagagattgc
      781 atgcagcttg ttcgacgatg tgtccatgct gacggtggcg gagctggtcg ccgaccccga
      841 gttggggttg gagttggtgg ccggacgcgg cggtgccgac cgggagatcg aggccgccgc
      901 ggtgtccgaa ctcgccaacc ccggcccatg gttgcagggc ggcgaactgc tcctgaccat
      961 cgggctactg cttccgcaga ccaccgacgg ttgtcggacc tatctgaccg agctcgacgc
     1021 cgctggcgtc caggccgtcg ggctgggcct gggcgccgaa ctcccccatc aacgggcgcc
     1081 cgcgcgcctc atcaccgccg ccgacgagat aggcatgccg ctgctgaccg tgcccgaccc
     1141 ggtcccgttt atcgcggtga cgaaggcggt attcgcatac cgtgcccgcg ccgagcgcca
     1201 cgagctggag tgggccctgg aaacccagcg cgcgttgacc gccgcggccg tcacgcccgg
     1261 tgggctgctc ggcatcctcg ccgcccatcg cgatgcgact ggccgatccg gtgtggtaat
     1321 cgacctcctc ggacgggtac tagccgagtc cgatcacggc ggtgaggggt tggtcagccg
     1381 gctgtccggg ctgctcgagt cgattcgcga tcagggtctg agcgccgcgg ctgtcgatat
     1441 cagcgacggc cgccgccgcg aattgcatgc cctcggcgcc cgccggctgc gtgcctggct
     1501 gctgatcgac ggccccgccg aactgcctgc ggcccatcag atttcgggcg atctggtgtc
     1561 gctgctgtcg ctggagttgg agcgacggca cgggctcaac accgcgcagc gccgaggtcg
     1621 tgcccaggtg ttggaccggc tggccagggc caccgtcgac gacgtggtcg cggcccggtg
     1681 gctgacgtcg gtggggctgc ccgacgccga ccttcgcgct gccgccgtcg ccgcacccga
     1741 cgacgccgac gacctggccg ccgatctgtt gatcaccgtg ccggacgctt tggttcgcgt
     1801 cgttggagaa gtggtggagt tggccgtgcc gctcgacacc gatctgccgg gcgtgctcgg
     1861 cacgctagcc cgcagtcgac cggcaggcat cggcatcgcc gtccggccgg gcgcgctggt
     1921 ggtttcgctg cggcaggccc gctcggcgct gcctgccagc cgggtgcaag gcagacacat
     1981 gcacgccgaa gagatcgcca gcagcaggct gttgctcaat acagttgggg cacaaccact
     2041 tcaggccttc gccgacgcgg tgctcggccc tctcgacgcg acggaccgtt cgggtgaact
     2101 gctgcgcggg ctcaccgcgt ttctcgagca caacggacat tggggatccg ccgccaacgc
     2161 tctgcgcgtc caccgacaca ccatgcgaaa ccgaatcgac gcggttgaac gattgaccgg
     2221 ccggcgcatg gattccgcgc aggaccgcca cgagttgtgg ctggcactgc gggcccgcga
     2281 cgccgcccgg atgtcgtccg actagcagac ggcgaaagcg caggtcgacg catcatgcgg
     2341 cgttacgctt actccgtggt ggtcaagggc cggcccgtct gcgtgccgga ggcggcgctc
     2401 gagagccgag tcccagcccg gacgcggctc catgccgaaa ccgtggcgcg ccacctcagg
     2461 gtcctgaccg tgaccacccg gatcggcgcg gccgtcagca tcttcttcgg cgtccaaggc
     2521 ctgatcgtcg gccaggacgt ggtgtggatc gggatggtga acctggccag cggggtgatc
     2581 ttcctgctga tgccgctgtt gtatcggttc ggcgaactgg ttgcgccact ggtgttctac
     2641 ttcatcgcct atgcctccat cacggtggtg tgttggcact tgggaacggg ctcgggtctc
     2701 cccttctatt acttggtcgc cgcgacgctg atggtgatga tcctcggaat cgatcacatg
     2761 gtgctcgcat cggtgctggc cgccctcggc gccgcaacgg tgatcacgct cgagatcgtc
     2821 gtgccctata acactggcgt gcaacctgat tgggcattcc gatcggggtt catcctctcc
     2881 gtcatatcgg cctggctgat ggtcatcgca gtggtgtggt acgcgctccg cgaaacccgt
     2941 cgtgcccggc tagcgatgga ggccgaatac gaacggtcgg aacgactgct caccaatatt
     3001 ctgcctgcca ccatcgccca acggctgaaa gacccgtcgc gcaacatcat cgccgacaag
     3061 tacgacgacg cctcgatcct gttcgccgac atcgccggct acaccaagcg tgccagcgac
     3121 accacgccaa gcgatttggt gcgcttcctc gaccggctct acaccgacct ggacgggctc
     3181 gtcgaccgtc acggcctgga gaaggtcaag accagtggtg attcctacat ggtggtcagc
     3241 ggtgtgccgg aaccccgcga cgatcacatc gaggcgctgg cctgcctggc actggacatg
     3301 gccgacgccg tgtctgacct gaaggatccg cagggtcggg aggtgccgct gcggatcggc
     3361 cttgccgccg ggccggtggt ggccggagtg gtcggcgcac gcaagttctt ctacgacgtg
     3421 tggggcgacg cggtcaacgt cgcggcgcgg atggaaacga ccgacgtcga ggggcggatt
     3481 caggtgccac aggacgtcta cgagcggctc aaccacgcgt ttctgctcga ggagcgcggc
     3541 aacgtcgacg tcaagggcaa gggcgtcatg cacacctggt atctggtcgg acgccggaac
     3601 gacgaagcgg tgcgcggcgc ggtcgagcgc gatgtcgctg cgacgggcag ttcctagcgc
     3661 aggacgctgg gtttgcgcgc caacagttcc agcagcgcag cggtcacctc gtccgggcgc
     3721 tcctccatga tgtagtggtc cccgccggtt agctgggtca gggtcgcacc cgggatgcgt
     3781 tcggccagtt cgcgcgcggt ggaaaacggg atatagcggt cgcggtcgcc ccaaatgatc
     3841 gccgtcgggc agtcgatctg ccctagtccc gcggccaggc cgggcacggc gggcacgtgg
     3901 tagttggtga agaagtcgac gaaggtctgc ctgccgggcg gggtgtccat ccagccgaga
     3961 tactcgtctt ccagtcgctc gtcgaaacag cccatcgcgc ggtacgggcg caacgcatcg
     4021 tgatgcagcc gccgcagcgg tagtcgccac agtagagcgg gagcgtggcg cgcgagccat
     4081 cgctggccct gtgagaagtg gtagaaccag ggccgaaaga tgttgtgcgc gcgggtattc
     4141 agcaatgcca accgcagcac gcgctccggg tggcgctgta catagccgag gccgaggaag
     4201 ccaccgtagt cgtgtgcgac gagattgaag cggtttaggc cgaacgaatc ggccagcgtg
     4261 ccgatccgct cgacctcggc gtcgtaggta gccggtgtgt cgggcgggtc cgcgcgtccg
     4321 aagcccggcc agtcggcggc gatgacgcgg tggtgtgtgg cgagcgcggg aatctggtgt
     4381 cgccagcacg atgcgctctg cggatagccg tgcagcagca gcaccggttc gccctcaccc
     4441 gcctcgatgt gcaccagatt cattttgttc aaactagtat aaaatttgcc ccgtgactag
     4501 acgccgcggc aggccccggg cctacgaccc ggatgtcgca atgcggcagg cgcgggacat
     4561 cttctggcgc ggcgggttcg cggcgacctc gctggatgac ctggcgacgg gcatgagcat
     4621 gaaccggccc agcatctatg ccgcgttcgg cgacaaacgc gcgctttacc tgcgggccgt
     4681 caccgagtac gcggagatga gcagcggctg gctggcgacg gcgctggcca ggccgcgatc
     4741 gttacgcgac gggctgcacg cggtctacag gtacgcgcgc gacttctatc aggcgggcga
     4801 tgacgcgcca cgcgggtgct tcctgctcgg caccgcggtc accgaggcga acggcgatcc
     4861 gcacgtgcgg gcgatcgtcg agcagacgat gtcggcgttc accgcgacgt tcgccgaacg
     4921 gttcgagcgc gccgaacgcg agggcgagct gtccccgcac gcgcccgacg cgctcgcaca
     4981 aatcgccacg gcggcgctga acacgctcgc ggtgcgcgcc cgcacgggtg cacccgcagc
     5041 ggtcctcgat gcactgatcg acgccacagt ggacgtgatc tgcgctagac cttccggttc
     5101 tccgacttga tcagccaggc cagcttctcc aggccgtcga tcagctcatg gaggatgtcg
     5161 gcagtgctgg ggtcctcggc atccaccgca tcatgcacgg tgcggagcgt gtcgaccgcc
     5221 gcgtaggtgc ggccggcgat caggtcgacg acctcggtgg tgttgtgctc gtaggccggg
     5281 aactgcggaa gcgtggtgtt ggccgcgacg gtgtcggacc ggccgtccgg cacggcgtcc
     5341 agcgcgcgca tccgctcggc gatggtgtcg ctggcctcac gggcgaagtc gacgagctcg
     5401 tcgagctgca ggtgaaggtc gcggaagttg gtgccgacga cgttccagtg cgcctgctta
     5461 ccctgcaggt gcagttcgat cagatcgatc agcacctttt gcaggttggc gctgagctcc
     5521 ggtgatgcct gaaatccctg tacttctgtt tctgtgcgac gtgttgtagt gcccatgcgt
     5581 aactcaacgc caagcgtttc tggaatcatt ccaaattagc gagatcttgt ttctccagca
     5641 gccgggcata accggcaccc atgccgagca agaccaggcc gacgacgatg aacaccacga
     5701 cgcggaagat gccgtcgagc gtgccgaggt cgaacaggaa cagcttggcc atcgccgcgg
     5761 ccaccagcgc cagcccgccg ccgatcggca gcgatcgctc cgctttggcc agtcgtgcgg
     5821 cgtagatgaa cagcgcggcc gccatcgcga tccagcagat cgtcgcggcc atgtgaccgg
     5881 caaagaaacc accgttgggg ccgccgatca gcacgccggc ggtcacggtg aacgcggtga
     5941 ccacgtaggc gatcactgcg gccgaaccgg cccagatagc cgctcggtcc tgctcccgga
     6001 cggtcttcca cgaccacgcg atcgtgatcg cgcataccac cagcagaacg ctcgagacga
     6061 gggtggaaac ggcgacggga gtgtcgagtt cggtggcgct catcagcgag ctcggcggcg
     6121 cgtagctcag gtagatgccg cccccgacga cggcgaagcc gatcgcggcc caccgcgcga
     6181 tgtcgtcgcg ccggcccgcg atcgcgacca tgaccgccat cgccagtagc accgggcccg
     6241 cgacgtagcc atcgaatgcc acggtcaccg cgatcagcgc cgcgatcgct gagagcgccg
     6301 accagatctg ccgaaccacg ccggtcacgt ttggcgggcc gagaagcacg attgccaaca
     6361 gcgcggcggc gagcgcggcc gccatcaatg cggccaccac ccggtcgacg gcgagcgaga
     6421 cgcatagcac cggcactacg cccgccgcgg tcaacagggc catcacggcc cgatttgccg
     6481 tgcgcggcaa caggattagc gctgccccga tggcgagcac ggcagcgatg ccgcacgcgc
     6541 ccgccagcca caggtcgtga tggtcgtcga agtacaccgc ggccagcgcc accagtatcg
     6601 ggaacgtcgg ggctgcgatc cgcgcggcgt gcagccaaat ccagtccttg cccagctgga
     6661 ccggcagcga cgccgccgac agcgccagca tgaagccgac gagcaacagg gtgatgccgt
     6721 cggtgaccac cggcgcgagc aagatcagcg gcaccagcac gagcacgccg agatgctggg
     6781 agtcccagcg ccgggccagg gtcaacccgc cgcccccgac gacagcggcg ataccgagcc
     6841 cgacgggtgc gggcacccag tgatagatcg tcgtgacggc gatgacgtcc atgtaggcgg
     6901 ccgcgatggc ggtggcggcc agggcgattg cgccgacccg tccgccgggt cggtcgtaca
     6961 gccaccacgc ggcggctatc agtgccgcgg cgagcacgac cccgcctgcg acgcggaact
     7021 ccgggcgcag aatccccgcc tgggccgcta gcacgagcag caatacgacg ccgaccagcg
     7081 tgaccgcgac gccagcgacg gcgagcacct tgccgatcca gccctcggac cgttcctgcc
     7141 gaggctgctt cgccggcgca ggtggtgggg gaacctgtgg cggcggcgac ggatagtgcg
     7201 cgtactgcgg cggatactgc ggccagtacg gcaccgccgg cgggacgtac ggcgcgggct
     7261 gcgggggcgc gacaggcgcg ggctgcgccg acagcagtcg gtcgagttct atcagatccg
     7321 cggacatgcg ggccagctgc tgggacatcg cagcgaagtc cgcggacaac cgggagatca
     7381 cggcgcgatg cggcggttcg gtcatggcaa catgctcgca gcagcaccgg catgtccgga
     7441 tgagtaggac tactccttat ccaacccgtg ttcgatcgcg tagcgggcca gctcgacccg
     7501 gttggcgact tgaagcttgc ggaacgtggc ctgcacgtgg ttctcgacgg tgcggtggct
     7561 cagcgagagc cgggcggcga tctgcttggc cgtcaaaccc ttggcgacgt ggcgaagtat
     7621 ctcggtttcg cgttcggtca ggctcggcat gtccggcccg ccaccggggc gctgcgcgat
     7681 gcgccggtac tcccccagca ccagtccggc caaccccggg gtgaacaccg cgcgtccttc
     7741 agcggtcgcg cgcacggcat cgccgagttc ttgcttcgac gcgctcttca cgagataccc
     7801 tgtggcgcct gcctttaccg cctcgaggac gtcgtcgcgc tcgtcggatg ccgagagcac
     7861 caggattcgc gacgacggcg acaccgcgag cacctcggcg gtggcctgag cgccgtcacc
     7921 gtcggacagc cgcatatcca tcaccaccac atcggggtgc accacggccg cgcggcgacg
     7981 cgccgccgcc accccgtcgg ctgtcgccac aacggagaag ccgtcctcgg cgagatcgcg
     8041 agctaccgcg tcgcgccaga tcggatggtc gtcgacgacc atcaccgtcg gggtaccgtc
     8101 agctcccatt ccgtcccgca ttccggcccg gtgttcaggt ttgcgctgcc gcccagccaa
     8161 ttcatccgtc ccacaatcga tttagcgatt ccaacgcggc cttctgcgac cgcttcctcg
     8221 agcctgccct cggcgatgcc gacgccgtcg tcgcggatgc tcacggtgac cgagtcgccg
     8281 aggtcctcaa gcaatacata ggcgcgcgcg gccggcccag cgtgtgacgc gacgttatcc
     8341 agcgcgttgg cggccgcagc gcacaac
//
LOCUS       DASRJA010000394         5986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2838060,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000394 DASRJA010000000
VERSION     DASRJA010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2838060"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="VFC01_17415"
     CDS             complement(<1..118)
                     /locus_tag="VFC01_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN81445.1"
                     /translation="MRIGLMVGSDKERSRADRLTGLLDDGKAAEAQGFASFWF"
     gene            complement(128..871)
                     /locus_tag="VFC01_17420"
     CDS             complement(128..871)
                     /locus_tag="VFC01_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81446.1"
                     /translation="MELPGTPSDQITEVLATGRGDVTTLFVSMARRHPDGADAEYLRW
                     HTLDHRPEQHRLSAVRASLRLVSTPACRSARATSRDPYDDIDHVMTYFFTDQSGMTGF
                     LDLAKALINAGRKLPLLPPVERGVYALEDKAAAPRVKVGADVLPWWPIRGVYLLLEAN
                     ASAATQLGDVDGVAGVWSALSQSVDARLASAPAGQTISYCFLDDDPVATADRLRPVLE
                     KRWSSNGIEPLFAAPFHVVVPYEWDRYVP"
     gene            complement(873..1781)
                     /locus_tag="VFC01_17425"
     CDS             complement(873..1781)
                     /locus_tag="VFC01_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN81447.1"
                     /translation="MLESEKVLITGATGKIAFPIARALAQRNEVWGAARLRDPADRDK
                     LAAVGVMPVALDMSTGDFSALPNDFTYVFHAAVDVGAGEWTDCVDTNAQKSGDLLYHC
                     RTAKGFVFCSTGSVYGYQGQRALRESDPPGVPLRANYSFSKIAAEAVCTWIAKQYQIP
                     LTTIRICSTYGPEGGAPADRLEMMLAHKPIRLYPDAPNNYNPIYEDDYVEFGIRAMEV
                     AATPPIVVNWAGSETVSVEDYCAYMGELVGIEPIFDYTPDAHTPLWPDVSYMHEVLGR
                     TKVPWREGFRRMIEARHPELTLTESN"
     gene            1838..3013
                     /locus_tag="VFC01_17430"
     CDS             1838..3013
                     /locus_tag="VFC01_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN81448.1"
                     /translation="MQLTFDAEVEEFRAEFSRFLDAHLPTEAQAAERSRSTAHVPQWA
                     RDWQRLQFDHGWLLPGNPPEFGGRNAGVLQQFVHREELSRRRIYHCFNPQGVGIIAAS
                     LLSFGTDEQKQQWAVPILRAEMTAALGMSEPGAGSDLAGLATRAVLDDDHFVVNGQKV
                     WTSGAHDADVLLTFVRTDPDAPRHKGISVLLIPTDTPGVERRPFASVVDRDSLDFNAV
                     FFTDVRVPAANLVGPLNGGWKVANGSLGHERTIMWMAFANRMEQLLEDYRPVGELNRD
                     RYATIAMDYQALRLLGSAALGQAARGERDIPGISVLKLLGSEAEQSAFRHALDAAGVD
                     GLSNSALTAPHNPYSPDLFTGSWFTRYVATYAGTISGGTSEIQRNIIAQRVLGLPAR"
     gene            complement(3017..4693)
                     /locus_tag="VFC01_17435"
     CDS             complement(3017..4693)
                     /locus_tag="VFC01_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN81449.1"
                     /translation="MTASDPHAPARFRLLASLIAGRALEIAEASTSEAAFTNGAVIFV
                     TADTSAEQRRREVVVQSSLLGAGSLEHRLVKALRARPSVARRYLTLEGRRALGQLGER
                     LPLAAALPIAPPSTTTPEESLEIARGRTKIDEPPDWFGAINPGRLLVLATGPGSRATH
                     RDLRLQFDPVGALEDDDEAPSEESKILKLFESPVVASRALSNILRKLFGGSPSAGDDA
                     AGGQLQVGSVRRAHDAGAVALPLPRRIHFSDDGKPGAAVGVGGALYPEWDVHHNSYRS
                     QWCRVIDFPLTAVADVSDADVQLDDVLWRRLSRIGLGLKVLRARADGDELDIEALIEL
                     SVDLRSGYSSTERVYLERRKLARDLGVLILLDASGSATDTDPKGLAVHDHQRRAAATL
                     AVTLEELGDRVAVYAFSSQGRHAVHLPAIKAFGQRFGATGRARLNQLQPSGYTRLGAA
                     IRGAGEILKSDAGTPNRLLLVLSDGFPYDDGYEGRYAEADVSRSLEELRTDGVGCLCL
                     SIGAAPDNGALERVFGSAGYASAPTLGDLSPQMDELFLSALMELAAPIRR"
     gene            complement(4690..5490)
                     /locus_tag="VFC01_17440"
     CDS             complement(4690..5490)
                     /locus_tag="VFC01_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019737080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HZN81450.1"
                     /translation="MTIQTPSQAPFYRALSDEVEVFRAAARRGLPVLLKGPTGCGKTR
                     FVEAMAHELGRQLITVAGHEDMTSADLVGRFLLKGGETVWVDGPLTRAVREGAICYLD
                     EIVEARQDTTVVIHPLADHRRELPIDRLGTTLPAAPGFQLVISYNPGYQSVLKNIKES
                     TRQRFIAIELDFPSAETETDIVAHEAGIDVQTARTLAMVGNAIRNLDGSPLREVASTR
                     MLILAGGLVAEGLNLRSAVQSAIVRVLSDDADVERALGELVDAFLPAT"
     gene            complement(5513..>5986)
                     /locus_tag="VFC01_17445"
     CDS             complement(5513..>5986)
                     /locus_tag="VFC01_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spirocyclase AveC family protein"
                     /protein_id="HZN81451.1"
                     /translation="PGAIRSVSVFAGTYHQWPIYEGLMWGGVQAAMCCLRYFTDDRGR
                     TIVERGLDNVRGGFARQQFTRFLAIFAAISGCFFFFYNVPAQWIAMHSDPWPDDHLKR
                     SYFNGGICGDGTDVPCPDPVLPMPTKRSGFINVDGELVLPDGAELPRTVPFDRGK"
BASE COUNT         1093 a         1886 c         1961 g         1046 t
ORIGIN      
        1 ggaaccagaa cgacgcgaat ccttgggcct cggctgcctt tccgtcgtca agcagtccgg
       61 tcagccggtc cgcacgtgac cgctccttgt cggagccgac catcaagccg attcgcatcc
      121 aagcccctca cggcacatag cgatcccact catacggcac cacaacatgg aaaggagcgg
      181 cgaagagtgg ttcgattccg ttgctgctcc atcgtttctc cagaaccggg cgcagccggt
      241 ccgcggtcgc aaccgggtca tcgtcgagga agcagtacga aatcgtctgg cccgcagggg
      301 cgctcgccaa ccgagcgtcg actgactgcg acagtgcaga ccagacaccg gccaccccgt
      361 cgacgtcccc cagctgagtc gcggcagaag cgtttgcttc cagcaggaga tagacaccgc
      421 gaatgggcca ccagggcagc acgtctgctc ctaccttgac ccgtggtgcc gccgctttgt
      481 cctcgagggc gtaaaccccg cgttcgaccg gaggcagcag cgggagtttg cggccagcgt
      541 tgatcagtgc cttggccagg tcgagaaaac ccgtcatacc gctctgatcg gtgaagaagt
      601 acgtcatcac gtggtcgatg tcgtcgtaag ggtctctgct cgtcgcacgc gctgaccggc
      661 aggccggcgt cgacaccaac cgcaacgacg cccgcacggc ggacagccgg tgctgttccg
      721 gccggtggtc cagggtgtgc cagcgcaggt attcggcgtc cgcaccgtcg ggatggcgcc
      781 tcgccatcga gacgaacagg gtggtgacat cgccgcgacc cgtggcgaga acttcggtga
      841 tctggtcaga cggcgtgccg ggcaattcca tgtcaattac tctccgtcag agtgagttcg
      901 gggtgacgag cctcgatcat ccgccgaaag ccttcgcgcc atggcacttt ggtacgtccg
      961 agcacttcgt gcatatagga cacatcgggc cacaacggtg tgtgggcgtc gggcgtgtag
     1021 tcgaagatcg gctcgatgcc gacgagttct cccatgtatg cgcagtagtc ctcgacgctc
     1081 acggtctcgc tgcctgccca attcaccacg atcggcggtg tagccgcgac ctccatggct
     1141 cggatgccga attcgacgta gtcgtcttcg tagatcgggt tgtagttgtt gggcgcgtcg
     1201 gggtagagcc gaatcggctt gtgggccaac atcatctcga gccggtcggc cggtgcaccg
     1261 ccctcggggc cgtaggtgga acagatccgg atggtcgtga gcgggatctg atactgcttg
     1321 gcgatccacg tgcagaccgc ctcggcggcg atcttggaga agctgtaatt cgcgcggagg
     1381 gggacacctg gcggatcgga ctcacgcaat gctcgctgcc cctggtatcc gtataccgac
     1441 ccggtggagc agaagacgaa gcccttggcc gtgcggcagt ggtagagcaa gtcgccggat
     1501 ttctgcgcgt tggtgtccac gcaatccgtc cactcgccgg cgccgacgtc gaccgcagca
     1561 tggaagacgt aggtgaaatc gttgggcagg gccgagaaat cgccggtgct catgtcgagc
     1621 gccacgggca tgacgccgac ggcggccagt ttgtcccgat cggccggatc tctcaggcgg
     1681 gcagcgcccc agacctcgtt gcgctgcgcc agcgcacggg cgatgggaaa cgcgatcttt
     1741 cccgtcgcgc cggtgatgag gaccttttcc gattcgagca tccaatcagt catagcgcac
     1801 cggcgcgcaa atgttaagta cttgatatgt tgggtgggtg caactcacgt tcgacgccga
     1861 ggtcgaggag tttcgcgccg agttcagcag gttcctcgac gcgcatctgc cgaccgaggc
     1921 gcaggccgcc gagcggtcgc ggtcgacggc gcacgtgccg cagtgggcgc gcgactggca
     1981 gcggctgcag ttcgaccacg gttggctgtt gccgggaaac cctcccgaat tcggtggccg
     2041 aaatgccggt gtgcttcagc agttcgtcca tcgtgaagaa ctgtcgcggc ggcgcatcta
     2101 ccactgtttc aacccgcagg gcgtcggcat catcgcggcg tcgctgctgt cgttcggcac
     2161 cgacgagcaa aagcagcagt gggcggttcc gatcttgcgt gcggaaatga cagcggccct
     2221 cggcatgagc gagcctggcg cgggatcaga tctcgccggg ctggccaccc gcgcggtact
     2281 ggacgacgac cacttcgtgg tcaatgggca gaaggtgtgg acgtctggcg cccacgacgc
     2341 cgacgtcctg ctgactttcg tgcgtaccga tcccgacgca ccgaggcaca agggcatcag
     2401 tgtgctgctg atccccaccg atacgccagg cgtagagcgc cgtccattcg catcggtggt
     2461 cgaccgagac agtctcgact tcaacgcggt gttcttcacc gacgtgcggg taccggcggc
     2521 gaacctcgtc ggtccgctga acggcggctg gaaagtcgca aacggatcgc tcggccacga
     2581 acgaacgatt atgtggatgg cgttcgccaa caggatggaa cagttgctcg aggattaccg
     2641 acctgtcggg gagctcaatc gggaccgcta cgcgaccatc gcgatggact atcaggcgct
     2701 gcgcctgttg ggctcggcgg cgcttggcca ggccgcacgc ggcgagcgag acatccccgg
     2761 catttcggtg ctcaagctgt tggggtcgga ggccgagcag agcgcgttca ggcatgccct
     2821 cgacgcggca ggcgtcgacg gcctcagcaa ttcagcgctc accgcaccgc ataaccctta
     2881 cagcccagac cttttcaccg gcagctggtt cacgcgctac gtcgccacct acgctggcac
     2941 catttcgggt ggcacgtcgg agatacagcg aaacatcatc gcgcagcgag tgctcggcct
     3001 gcccgcgcgc tgacgatcac cgccgaattg gtgcagcgag ttccatcagc gccgacagga
     3061 aaagctcatc catctgcggg ctcaaatccc ctagggtcgg cgcgctggca tagcccgccg
     3121 agccgaacac ccgctcgagc gcgccattgt caggcgcggc gccgatggaa agacaaaggc
     3181 agccaacgcc atccgtgcgc agctcttcga gcgatctgct gacgtcggcc tctgcgtaac
     3241 ggccttcgta accgtcgtcg tagggaaatc catccgaaag gacgagcagc agccggttcg
     3301 gcgtgccggc gtcactcttg agaatttcac ctgcacctcg aatcgcggcg ccaaggcggg
     3361 tgtagccgga cggctgaagc tggttgagcc gggcacgccc agtggcaccg aagcgctgtc
     3421 cgaaggcttt gattgcgggt aagtggacag cgtggcggcc ctgcgaggag aacgcataaa
     3481 cggcgacgcg gtcgcccagt tcttcgagtg tgacggccag tgtggcagcg gcgcgacgtt
     3541 gatggtcgtg tactgcgaga cccttcggat cggtgtcggt ggccgacccg gaggcatcga
     3601 gcaggatgag gacgccgagg tcgcgagcga gtttgcgacg ttccaggtag acacgttccg
     3661 ttgacgaata cccggaacgc aagtcgacgg acagttcgat tagcgcctcg atgtcgagtt
     3721 cgtccccatc ggcacgcgca cgtaaaacct tgaggccaag accgattcgc gacaggcgtc
     3781 gccacagcac gtcgtcgagc tgcacatctg cgtctgacac gtcggcgacg gccgtcagcg
     3841 gaaagtcaat gacccggcac cactgcgacc ggtagctgtt gtgatgaaca tcccactctg
     3901 gatagagagc tccgcctaca ccaacggcgg ctccgggttt cccgtcgtcg ctgaagtgga
     3961 tccgtctggg gagcggcagg gcgacggcgc cggcgtcgtg cgccctcctc accgagccga
     4021 cctgcaactg gccgcccgcg gcatcatcgc ccgccgacgg tgaaccgcca aacagcttgc
     4081 gcaggatgtt cgacagcgcc cgagaagcga caacgggact ctcgaacagc ttgaggatct
     4141 tgctctcctc cgatggtgcc tcgtcatcgt cctcgagcgc gccgacggga tcgaactgca
     4201 ggcgcagatc tcggtgggtg gcccggctcc caggaccagt ggcgagcacc aagagtcgac
     4261 cggggtttat ggcgccgaac caatccggcg gttcgtcgat tttggttcgc cccctggcta
     4321 tttcgagcga ttcttcgggg gttgtggtgc tcggtggtgc gatgggaagc gcggcggcta
     4381 gtgggagccg ttcgccgagt tgaccaagtg cgcgacgacc ttcgagcgtc aggtacctgc
     4441 gggcgacgga tggacgtgct cgcaacgcct tcaccaaacg atgctccaga ctgccggcac
     4501 cgagcagcga actctgcacc accacctcgc ggcgtcgctg ctcggcggag gtgtcggccg
     4561 tgacgaagat gaccgcgccg ttcgtgaatg cggcttcgct ggtcgatgct tcggcgattt
     4621 cgagcgccct gccggcgata agggacgcga gaagtcgaaa acgggcaggg gcgtgcggat
     4681 cagatgcggt cacgtcgcgg gcagaaaggc gtcgacgagt tcgccgagcg cacgctcgac
     4741 gtccgcatcg tcggagagca cccggacgat cgccgattgg acggcactgc gcaggttgag
     4801 cccttcggcg acaaggccac ccgcgaggat caacatccgg gtggacgcga cctcgcgcag
     4861 cggtgagcca tccaggtttc ggatggcgtt gccgaccatg gcgagagtcc gcgcggtctg
     4921 aacatcgatt ccggcttcat gggcgacgat gtccgtctcg gtctcggcag acgggaagtc
     4981 gagttcgatc gcgatgaagc gttgacgggt tgactccttg atgtttttca aaacgctttg
     5041 atagccaggg ttgtaggaga tcaccagctg gaaacccggt gcggcgggca gcgtcgtgcc
     5101 gagccgatcg atcggtaact cgcggcgatg gtcggcgagc gggtgaatca ccacggtggt
     5161 gtcctggcgc gcctcgacga tctcgtccag atagcaaatg gccccctcgc gaacggcccg
     5221 cgtcaaggga ccgtccaccc aaaccgtttc cccgcctttg agcaggaagc ggccgaccag
     5281 atccgccgac gtcatgtcct catggcccgc cacggtgatc agttggcgtc ccagctcgtg
     5341 cgccatcgcc tccacgaagc gggttttccc acaacccgtc ggacccttca gcaacaccgg
     5401 caggccgcgc cgagccgcgg cacggaacac ctccacctcg tcgctgagcg ctcggtaaaa
     5461 cggtgcctgc gaaggggttt gaatggtcat cggccgactg ctggtcaacg actcacttcc
     5521 cgcggtcgaa cggtacggtc cgcggaagct cggccccatc gggcaggacg agttcgccgt
     5581 cgacattgat gaagcccgac cgcttcgtcg gcattggcag caccgggtcc ggacagggta
     5641 cgtcggtccc gtcgccgcag atgcctccgt tgaaatacga gcgcttcagg tggtcatctg
     5701 gccatgggtc ggagtgcatg gcgatccatt gcgcgggaac gttgtagaaa aagaagaagc
     5761 acccgctgat cgctgcgaat atcgcgagga atcgggtgaa ttgctgccgc gcgaatccac
     5821 ctcgcacgtt gtccagccct cgttcgacga tggtgcggcc acggtcgtcg gtgaaatacc
     5881 gcaggcagca cattgcggcc tggacgccgc cccacatcaa tccctcgtag atcggccact
     5941 ggtgatacgt gccggcgaac acggacaccg agcgaatcgc gccggg
//
LOCUS       DASRJA010000395         3604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1302909,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000395 DASRJA010000000
VERSION     DASRJA010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302909"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFC01_17450"
     CDS             <1..788
                     /locus_tag="VFC01_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN81452.1"
                     /translation="TGGPATGRAVMAAAAARLTPVLLELGGNDAAIIAPDMTISEQLV
                     EQLVTATYTTCGQVCMAIKRLYAPRDRVGELAEAVLARCQREVVGDGLADEVTLGPLH
                     TAAGRERVEKLVKDAAAQGATVRTAGRLREEDADAGGYFVLPTVVTDVAPDSALACEE
                     QFGPVLPIFGYGEIDEAVAAANATAFGLTASVWTGDEALADKVTGQLIAGTVSVNCHG
                     MAAQDPRVPFGGVGQSGLGRELGVDGIRAFSQARGYVRQPAPH"
     gene            1045..1272
                     /locus_tag="VFC01_17455"
     CDS             1045..1272
                     /locus_tag="VFC01_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81453.1"
                     /translation="MTDREIIERLGVLEQLVRHLYEKTGVPIPDLQTLAQVSPNVRQL
                     LASGNKIAAIKAYRDETNVDLATATRMIESL"
     gene            complement(1275..2693)
                     /locus_tag="VFC01_17460"
     CDS             complement(1275..2693)
                     /locus_tag="VFC01_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HZN81454.1"
                     /translation="MSPRLRIASATVASAAAIGLCTAIGVAPPAGAEPCTDAAAAAQP
                     AAPAAPAQPLPTTGGPPRGHRPPNANTSAPLPRLGQISRALLNAFTPQSGQVQKQAGV
                     APQPTPPNANQQPPAAAQPVPEAAPAPPPAPAQPADPPGTSLVGWVTGPNSPNNTLER
                     FAVSGTDLGIMWDNGSRQVLMAFGDTSGYCSAPGHQWRYNVLMRSQDGALANTISVAN
                     SPMWARGISRQIINTTKWAPDETGIIPTAGISVGGKQYLNYMSIKSWDANGAWTTNYS
                     AIAVSPDNGDRWGVYPGSVRTPRPDAIPGARHVAGNENFQQGAFLKPGPGDPYIYSYG
                     TPAGRAGAAYLARVVPDGLPDVTKYEYWNSDQNAWVPGNPASATPVVPGPVGEMSAQY
                     NTYLRQYLIMYCNGMNDVVFRTAPAPQGPWSPEQMIVPSNQFPGGIYAPFLHPWSTGR
                     TLYYNLSEWNDYNVMLMKTVLP"
     gene            3059..>3604
                     /locus_tag="VFC01_17465"
     CDS             3059..>3604
                     /locus_tag="VFC01_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZN81455.1"
                     /translation="MALLPDPTPRERQHILISVDDHVIEPPDMFDGRLPIALADRAPK
                     IIETDGRQVWRYEDREYPNIGLNAVIGRPKEEWSMEPARFDEMRRGCWDIDARIADMD
                     LAGIWASLNFPSLIAGFAGTVFWKSDDPELGLAVLRAWNDWHHEVWAGSYPDRIIPLQ
                     LPWLADPQLAADELRRNAERGF"
BASE COUNT          595 a         1094 c         1278 g          637 t
ORIGIN      
        1 tgaccggtgg ccctgcgacg ggccgcgcgg tgatggcggc cgccgcggcg cggctgacgc
       61 cggtgctgct tgaactcggc ggcaatgatg cggcgatcat cgcgcccgac atgacgatca
      121 gcgagcagct cgtcgagcaa ctggtgacgg cgacgtacac cacctgcggg caggtgtgca
      181 tggcgatcaa gcggctgtat gcgccacgcg atcgcgtcgg cgagctcgcg gaggcggtgc
      241 tggcgcggtg tcagcgggag gtggtcggcg acggtctggc ggacgaggtg acgctcggac
      301 cgctgcacac cgcggcgggg cgcgagaggg tcgaaaagct ggtgaaagat gctgcggcac
      361 aaggagcgac ggtgcgcacg gcgggccggc tccgcgagga agacgccgac gcgggcgggt
      421 acttcgtgtt gccgacggtc gtcacggatg tggcgccgga ctcggcgttg gcgtgcgagg
      481 agcagttcgg tccggtgctg ccgatcttcg gctacggcga gatcgacgag gcggtggccg
      541 cggcgaacgc gacggcgttc ggtctgaccg cgtcggtgtg gacaggcgac gaggcgctgg
      601 ctgacaaggt gacgggtcaa ctgatcgcgg ggacggtgag cgtgaactgt cacggcatgg
      661 ctgcgcagga cccgcgggtg ccgttcggcg gcgtggggca gtcgggcctc ggacgcgagc
      721 tcggggtcga cgggatccgg gcattcagcc aggcacgcgg ctacgttcgc cagccggcgc
      781 cgcattagta gcgtctgatc ccgacttgtc ggagggacac ggcacgcttc ccgaaagcgt
      841 caacacatga tgacgccgcc gagaagggtg gttctcccat ggagaatggc agcaagccgt
      901 ggtcgaacgt gcccgggccc gttacgaaga cgcggtgcgt agcgcccgca cagcggggca
      961 ctcatggagc gaaatcggcc ggattcttgg ggtttcgaag caagtgttgc accgtcggtt
     1021 cagccaaggt gacacactgt cgcgatgaca gacagggaaa ttatcgaacg gctgggtgtg
     1081 ctcgagcagc tcgtccgaca cctctacgaa aagacggggg tgccaatacc cgatctgcag
     1141 accctcgccc aggtgtcgcc caatgttcgg cagctgctcg cctccggcaa caaaatcgcc
     1201 gcgatcaagg cctaccgcga cgaaacgaat gtcgatctgg ctaccgcaac ccggatgatc
     1261 gagtcgctct gaccttatgg cagcacagtc ttcatcaaca tcacgttgta gtcgttccac
     1321 tccgagaggt tgtagtacag cgttctaccc gtcgaccacg ggtgcaggaa cggtgcatag
     1381 atgccgccgg ggaactggtt cgacgggacg atcatttgtt ctggactcca cggtccttgc
     1441 ggcgcaggtg cggtcctgaa cacgacgtcg ttcatgccgt tgcagtacat gatcaggtat
     1501 tgcctgaggt aggtgttgta ttgggccgac atctcgccca ccggtccggg gactaccggc
     1561 gtcgcagagg ccggattgcc tgggacccag gcgttttggt ctgagttcca gtactcgtac
     1621 ttggtgacgt ccggcagacc gtccgggacg actcgcgcca gatacgccgc gccggcgcgg
     1681 ccggctggcg tcccgtacga gtagatgtag gggtcgccgg gtccgggctt gaggaacgcc
     1741 ccctgctgga agttctcatt gcccgcaacg tgccgggctc ccgggatggc gtccggtcgt
     1801 ggagtgcgga cggatccggg gtagacaccc caacggtcgc cgttgtccgg cgacaccgcg
     1861 atcgccgagt agttggttgt ccacgcgccg ttggcatccc aactcttgat ggacatgtag
     1921 ttgaggtact gctttccgcc gacggagatg ccggcagtgg gaatgatccc ggtctcatcg
     1981 ggcgcccact tggtggtgtt gatgatctgt ctggagatgc cgcgggccca catcggtgaa
     2041 ttggccacgc tgatggtgtt ggccagcgcg ccgtcctggc tgcgcatcaa cacgttgtac
     2101 cgccactggt gtccgggggc gctgcaatag ccggacgtgt cgccgaacgc catcagaacc
     2161 tggcgagagc cgttgtccca catgatcccc aggtcggtgc cggagacggc gaaccgttcc
     2221 agcgtgttgt tcgggctgtt cggtccggtc acccaaccga cgagcgatgt gccgggcggg
     2281 tcggccggct gtgccggtgc cggcggcgga gctggcgctg cctcaggaac cggctgggca
     2341 gcggcgggcg gctgttggtt ggcgttgggg ggagtgggtt gaggcgccac gcccgcctgc
     2401 ttctgtacct gcccggactg gggagtgaac gcgttgagga gggctctcga tatctgaccc
     2461 agccgaggca gcggtgcgct cgtattcgca ttgggtggcc ggtgccctct cggtggaccg
     2521 ccggtcgtgg gcaacggctg agccggggct gcgggagcag caggttgggc tgccgcggcc
     2581 gcatcggtgc acggctcggc ccccgcgggt ggcgcgacgc cgatcgcggt gcacagtccg
     2641 attgcagccg ccgacgccac cgttgctgag gcgattcgca gacgcggcga catgacacac
     2701 ctttcaaagg ggcatgacgt cgctttgacg tcagcagtca actgatgtga cgatagtgat
     2761 ccctgaggct tgtgtgctca gtgatgtacg gataggtatc catccgtgac cgtgtcgcta
     2821 tccatcgatg taattcgtcc ccgcggccgc cgcggctggg agtttgctga aggtccttcg
     2881 aacagccgag tccaagcgag aggcgtgtgc ccatggtcct ccaccaccca gctgacccct
     2941 cacaccgatt cgtcgtggat tgggatgcgc cccgggcatg actgcgccag tctgcgatct
     3001 cgctgttttt acacaaatag cattgacagc cgtcgaaaag cgacgatagt cgtcaggtat
     3061 ggcgctgctg cccgacccga cgccccggga gcgacagcac attctgatct cggtggacga
     3121 ccacgtcatc gagcctcccg acatgttcga cggccgcctg cccatagcgc tggctgaccg
     3181 ggcgccgaag atcatcgaga ccgacggccg tcaggtgtgg cgctacgagg accgcgagta
     3241 cccgaatatc ggcctcaacg cggtcatcgg tcggcccaag gaagaatgga gcatggagcc
     3301 cgcccgcttc gacgaaatgc gccgtggctg ttgggatatc gacgcccgga tcgccgacat
     3361 ggacctggca gggatctggg cctcgctgaa cttcccatcg ctcatcgccg ggttcgcagg
     3421 gacggtgttc tggaagagcg acgaccccga gctcggcctc gcggtgctgc gcgcgtggaa
     3481 cgactggcat cacgaggtgt gggccggctc gtatcccgat cggatcattc cgctgcagct
     3541 gccgtggctg gccgacccgc agctcgccgc cgacgagctg cgccgtaacg ccgagcgcgg
     3601 cttc
//
LOCUS       DASRJA010000396         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2967787,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000396 DASRJA010000000
VERSION     DASRJA010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2967787"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFC01_17470"
     CDS             <1..731
                     /locus_tag="VFC01_17470"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HZN81456.1"
                     /translation="MATLVDALRVLRSRLRRGLTRASVGAFHQGLVRIGLDAGQRYGF
                     ALAGGYAVQAAGFLKRPTKDIDLFTAWERRGDFETAARAIVDAYLAAGLSVQAERRHD
                     TFTRLTVSDGVQTAKVELGVDLRRNEPVRTWIGPVLHPDDAAANKIRALYERGHASDF
                     IDIDAALRSGRYDRSTLLQLGERSDITFDKSVFADALAQAQLLDADDFAQYGLVGADL
                     DGLRHRFALWRAELLGAAGPTRTQ"
     gene            769..1071
                     /locus_tag="VFC01_17475"
     CDS             769..1071
                     /locus_tag="VFC01_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81457.1"
                     /translation="MCSPVRPIKLSAAGRSFLADAVRSGTLSATLGALMLFAINLFQL
                     LSLHDYTLTEHSDIAAFPKSALPDAARYLLSDALSGENHLRTHPRSPRSARRSSDR"
     gene            1864..>2199
                     /locus_tag="VFC01_17480"
     CDS             1864..>2199
                     /locus_tag="VFC01_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016690762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81458.1"
                     /translation="MPTQDDVLGYFNTLSNWGRWGDDDELGTLNHITDDVRLAAARAV
                     RHGRSVSCAWEVAVPQDMERSTTTCPCAADMPGAQDMPVPAFHADRRWGFSSERLGIT
                     FHGNTITHID"
BASE COUNT          387 a          735 c          712 g          365 t
ORIGIN      
        1 tgatggcgac gctcgtggat gcgctgaggg tccttcggag ccggcttcgc cgcgggctca
       61 cgcgggctag cgtgggcgcc ttccaccaag gtctggtccg gatcggcctc gacgccgggc
      121 agcggtacgg gtttgccctg gccggcggtt acgctgtgca ggccgcgggc ttcctcaaac
      181 gacccaccaa ggacatcgac ctgttcacgg cctgggaacg gcggggcgac ttcgaaaccg
      241 cggcccgggc aatcgtcgac gcatatcttg ccgccggtct aagcgtgcag gccgaacgcc
      301 gccacgacac ctttacccgg ctaaccgtct ccgatggcgt acagaccgca aaggtcgaac
      361 tcggcgtgga cctgcgccgc aacgaaccag tcaggacttg gatcgggccg gtgctgcacc
      421 ccgacgacgc ggccgccaac aagatccgcg cactctacga gcgtggccac gcgtctgact
      481 tcatagacat cgacgcggcg ctgcggtcgg gtcgctacga cagatcgacg ctcctgcagc
      541 tcggcgagcg ctcggacatc accttcgaca aaagcgtctt cgccgacgcg ctcgcgcagg
      601 cgcaactgct cgacgccgac gacttcgcgc agtacggcct cgtcggcgcg gacctcgacg
      661 gtctgcgtca ccggttcgcc ctttggcgcg cagagctgct cggcgccgcc ggaccgacca
      721 ggactcagtg atctgtcgag cggcggaatg tggcgccggc agcggccgat gtgttcgccg
      781 gttcgcccga tcaaactgtc ggccgccggg cgatctttcc tcgcagacgc ggtgcgctcc
      841 ggcacgctct cggccacgct gggcgctttg atgctcttcg ccatcaacct cttccagctg
      901 ctgagcctgc acgactacac gctcaccgaa cactccgaca ttgcggcctt tccgaaaagc
      961 gcactgcccg acgccgcgcg ctacctgctc agcgacgctc tcagcgggga aaatcatctc
     1021 cggactcatc cacgatcacc acgttctgct cgccgtagct ccgatcggta gcctggcggg
     1081 caccgggatc gttagaaggc cgggtgtatc cgctgttgca cgtccacagt agagcggaaa
     1141 ggcctccgca tttcgtaggg ccgagaatct cgacgatgac gttaatgtgg cgcaagcgaa
     1201 tgcgacgatg gcgacgcctg aggttcgccg agacatggtc cgccataggc ttgtaaggcg
     1261 gcactccaaa actgcgtctg ccgtaacggt gtcgagagtg gaatgccgcc aagtatcagc
     1321 cgatcaacgc aatcgtggcc gacagcgccg gaccgcgttg ggtattgggt tgtgtcgatc
     1381 cggacacaca ccaaacccca acaccgaggt caacgagtta tatggagtcc tgttggggtt
     1441 cgcctcggta gcgacagaac cccaacaccg aaccaccggc tgcctgaccc tcaagttggg
     1501 ggtactcccc cgcaagccgg attcagatgg gcttgatcac agatcacagg atccagtcaa
     1561 tgcgtcgacc tccgccgaca tcgcggtgga gatgatagag agggcagtgg agatcttgcc
     1621 gtccgtgtcg ctgcgggcgt tgccggcgcc acggcgggac ttgcacggct ggcgggcgcg
     1681 ttagctggag cggctatgac gccggtcctc gagcgcggtc ctggccgact gctggcccgc
     1741 tgatgtagtg tcatgccagt tcagcgcgcc ttggcaggtc aacggagagg cgggataccc
     1801 tacccgcgtg cctcgaaccg cgtgaccgca ggagttgatg ctgggatgac ggcagggcga
     1861 ccgatgccca cacaggacga cgtgctcggg tacttcaaca cgctgtcgaa ctggggacgg
     1921 tggggcgacg acgacgagct cggcactctg aaccacatca ccgacgacgt ccggctggcg
     1981 gcggcgcggg ccgtgcgcca cggcaggagc gtgtcgtgcg cctgggaggt tgccgtaccg
     2041 caagacatgg agcggtcgac gacgacgtgc ccgtgcgccg ccgacatgcc aggtgcccag
     2101 gacatgccgg tgcccgcctt ccacgccgac cggcgctggg gcttttcgtc cgagcggctc
     2161 ggcatcacgt tccacggcaa caccatcacc cacatcgac
//
LOCUS       DASRJA010000397         4338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2484426,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000397 DASRJA010000000
VERSION     DASRJA010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484426"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VFC01_17485"
     CDS             <1..429
                     /locus_tag="VFC01_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN81459.1"
                     /translation="DIRYASADAKFSVREVKLAIVADVGSLARLPLILNDGHLRELAL
                     TGKDIDATRAEKIGLVNDVYADAEASLAAAHETAAQIATNPPLVVNGIKDVLDQQRIA
                     RVSESLRYVAVWNAAFLPSKDLTEGLTATFEKRAPNFTGE"
     gene            complement(416..1324)
                     /locus_tag="VFC01_17490"
     CDS             complement(416..1324)
                     /locus_tag="VFC01_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN81460.1"
                     /translation="MRVLVAGATSVPGLPLLRELNARGHDVVGLTRQSEKTPQIEQAG
                     AKPVVADVFDAGQINTVVADIEPEVVVSLLTTLPKRGPMRIKDFEPARALWSRGAPNL
                     VAAAQRAGVRRVVAESVIFAYGYPTTGPALMDETDPYPGPPPPGGEAMLKALRDMEQT
                     VLTSSDHSDTEGIVLRYGAFYGPGVPHDEMFTRLAKWWAMPALTGNGIVSWIHIDDVA
                     KATAAAVEQGRGGQIYNIVDDRPQSFGDYIRELSAKLHRPRPLPVPRRLFGLVAPYGA
                     IAFGDTWLPLSNAKAKADLGWTPIPR"
     gene            complement(1324..1731)
                     /locus_tag="VFC01_17495"
     CDS             complement(1324..1731)
                     /locus_tag="VFC01_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81461.1"
                     /translation="MATTPTRARVRFVQVTLIVFGVYSVLVGLFMLVAPGTFFDTLGN
                     FGARNDHYIFDNASFELPLGLMMLAAARWRSWRIPALAFATLHWGLHALSHIIDPHHA
                     AGDAIGWLEAAGLVVTTALLAIALRASIVEERS"
     gene            1778..3163
                     /locus_tag="VFC01_17500"
     CDS             1778..3163
                     /locus_tag="VFC01_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZN81462.1"
                     /translation="MAISGTTSGPELLAELDRAAPEPLHRQLANGLRDAIRTGRLAPA
                     TRLPSTRVLAADLGVSRRLVVDAYSQLAAEGFLLSRHGSGTRVATVDAASAPQRDAGE
                     PPRRSDIDFAPGSPDLGSFPRHAWLRALRQGLAEIESHAFGYVAPQGLHSARVAVADY
                     VRRTRGVLADPQHIVLCSGATQAIALLASVLSDETLAMEDPGFWLHRMVLRHNGIDTV
                     PVPVDGDGIDVDALADSDATAVLATPAHQSPTGVVLSPARRTALVQWARGDHLVIEDD
                     YDAEYRYDRAPVGALQGIAPDRVVYVGSTSKTLAPGLRIGWMVLPTHLVGPVTLSKGL
                     SDTGSSVMDQIAFAKLLTSGGYDRHLRQMRRRYLARRNTLLRALARYFPEATVLGAAA
                     GVHLTVRFPDGFPISDLVDRAAKMRVKVEPLAPCYADPGSAPPGLMLGYANLTESQIV
                     TGVQTLARATR"
     gene            complement(3932..4018)
                     /locus_tag="VFC01_17505"
     tRNA            complement(3932..4018)
                     /locus_tag="VFC01_17505"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3982..3984),aa:Ser,seq:tga)
     gene            complement(4094..>4338)
                     /locus_tag="VFC01_17510"
     CDS             complement(4094..>4338)
                     /locus_tag="VFC01_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN81463.1"
                     /translation="ELQGLVRRGASPDDGRGVLASITDEGRVAVTEAMLTYGEGVRAH
                     FLGRLSRPQIAAMGENCRRISVALKSGSPPAKLGRV"
BASE COUNT          760 a         1441 c         1400 g          737 t
ORIGIN      
        1 gacatccggt atgccagcgc ggacgcgaag ttctcggtac gcgaggtcaa gctggcgatc
       61 gtcgcggacg tcggcagcct cgcgcgctta ccgctgattc tgaacgacgg tcatttgcgg
      121 gaattggcgt tgacgggcaa ggacatcgac gcgacgcggg ccgagaagat cggtctggtc
      181 aacgacgtct atgccgacgc cgaagcgtcg ctggctgccg cgcatgaaac ggccgcgcag
      241 atcgccacca atccaccgct tgtcgtcaac ggcatcaagg acgtgctcga tcagcagcgc
      301 atcgcccgcg tctcggaaag cctgcggtac gtcgccgtgt ggaatgcagc gttcctgcca
      361 tcgaaggatc tgaccgaggg cctcaccgcg acgttcgaga agcgggcgcc gaacttcacc
      421 ggggaatagg cgtccagccg agatcggcct tggccttggc attcgacagc ggcagccagg
      481 tatcgccgaa cgcgatcgca ccgtacgggg caacgagccc gaaaagccgc cgtggcacgg
      541 gaagcggcct gggccggtgc agtttcgcgg acagctcgcg gatgtagtca ccgaacgact
      601 gcggacggtc gtcgacgatg ttgtagatct gaccgccgcg accctgttcg acggctgccg
      661 ccgtcgcctt tgcgacatcg tcgatgtgaa tccacgacac gatcccgttt ccggtcaggg
      721 cgggcatcgc ccaccacttc gccaagcgcg tgaacatttc gtcgtggggc actcccggtc
      781 cgtagaaagc gccgtagcgc aacacgattc cctcggtgtc gctgtggtca cttgacgtca
      841 ggaccgtctg ttccatgtcg cgcaaggcct tgagcatcgc ttcgccgccg ggcggcggcg
      901 gccctggata cgggtcggtc tcgtccatga gcgcgggccc ggtcgtgggg tagccgtacg
      961 cgaagatcac cgactcggcg acgacccggc gcacacctgc gcgttgcgcg gccgccacca
     1021 ggttcggcgc cccgcggctc cacagcgcac gcgcgggttc aaagtccttg atccgcatgg
     1081 gcccccgctt cggcagtgtg gtcagcaggc tgaccacgac ctcgggttcg atatcggcga
     1141 ccacggtgtt gatttgcccc gcgtcgaaga catcggccac cactggtttg gcgcccgcct
     1201 gctcgatctg cggcgtcttc tccgactgac gagtcagccc aacgacgtcg tgaccacggg
     1261 cgttcagctc ccgcaacagc ggaagtccgg ggacgctcgt tgcgcctgcc actagtactc
     1321 gcattagctc ctctcctcaa cgatgctggc gcgcaacgca attgccagca gtgcagtggt
     1381 caccaccaat ccagcggctt ccagccagcc gatcgcatca cccgccgcgt ggtgggggtc
     1441 gatgatgtga ctcaacgcgt gtagccccca gtggagggtg gcaaacgcga gcgccggaat
     1501 ccgccatgac cgccaccgcg ccgcggccag catcatcagg ccaagcggaa gctcgaatga
     1561 ggcgttgtcg aagatgtagt ggtcgttgcg cgcgccgaag ttgccgagcg tgtcaaagaa
     1621 cgtccccggc gcgacgagca tgaacagccc gaccagcacc gaatacaccc cgaacacgat
     1681 cagggtcacc tggacgaagc ggactcttgc gcgcgtcggt gtggttgcca tacgttcacg
     1741 ctagatcgct attggcctca agataggctc cactttgatg gcaatttcgg gtaccacttc
     1801 tggtccggag ctgttggccg aactcgaccg cgcggcgccc gagccgctac accggcagct
     1861 cgcgaacggt ctgcgcgacg cgatccgcac gggccggttg gcgcccgcga cgcggctgcc
     1921 gtcgacccgg gtgctggccg ctgacctcgg cgtctcacgt cggctcgtcg ttgacgcgta
     1981 cagccagctg gccgccgagg gattcctgct cagcaggcac gggtccggaa cccgtgtggc
     2041 gacggtcgac gccgcgagcg cgccgcaacg cgacgcgggc gagccaccaa ggcggagcga
     2101 catcgacttc gcgcccggct cgccggatct cggcagcttt ccacggcatg cgtggctgcg
     2161 ggcactccgg caaggcctcg ccgagatcga gtcgcacgca ttcggctacg tggccccgca
     2221 agggctgcac tccgcgcgcg tcgcggtggc ggactatgtg cggcgcacgc gtggcgtgct
     2281 ggccgacccg cagcacatcg tgctctgctc cggggcgaca caggcgatcg cgctgctcgc
     2341 gtcggtgctc tccgatgaga cgctggcgat ggaggacccc ggcttctggc tgcaccggat
     2401 ggtattgcgc cacaacggta ttgacactgt tccggttcct gttgacggtg acggtattga
     2461 cgtcgacgcc ctggcggaca gcgatgcgac ggcggtgctc gccactcctg cccatcaatc
     2521 gccgaccggc gtcgtgctct cccccgcgcg acgcaccgcg ctggtgcagt gggccagagg
     2581 ggatcacctc gtgatcgagg acgactacga cgccgaatac cggtacgacc gcgcccccgt
     2641 tggagcgctg cagggcatcg cgcccgaccg tgtggtctac gtcggctcga cgagcaagac
     2701 gctggcaccc gggctgcgga tcggatggat ggtcttgcct acgcatctcg tcgggccggt
     2761 cacgctgtca aaaggcctgt ccgacaccgg tagttcggtg atggatcaga tcgcgttcgc
     2821 gaagctgctg acgtccgggg gatacgatcg acacctgcgc cagatgcgcc gccgctacct
     2881 ggcacgccga aatacattgc tgcgggcctt ggctcggtac ttcccggagg cgactgtgct
     2941 gggcgcggcc gctggcgttc atctcacggt gcgatttccg gacggctttc ccatttcaga
     3001 tctggtcgac cgcgccgcga agatgcgagt gaaagtcgag cctctggcac cgtgttacgc
     3061 cgaccctggt tcagctccac cgggattgat gctcggctac gcgaacctca ccgagtcgca
     3121 gatcgtgacg ggcgtgcaga cgctggcgcg agctacgcgc tagctgcctc cgccgcagcc
     3181 tccgccgcca caaccgccgc cgccgcagcc ggctccacct ccgggatcaa tggagtcagc
     3241 gcccaccgct cccgcagtgc cagccgccca taccctgcgg cgccgttggg tccgtcgcgg
     3301 atgtctggct aggcgctgtg agtgtgctaa ccaaagggga tcgtcggacg ggagatggac
     3361 gctcgggtcc ccgggatgcc ggacgttgga ccgcgcgcgt gaaggacgct ttaacaccca
     3421 gatgatgacg gcaacgatca ccaaagctag ggcgataagg ccaatcctgc cagtcatatt
     3481 caaaggctac gccggttcgc tacccgggcc tagggggcaa ttcgatacca caagttggtg
     3541 atatgtccgg cgcgcgtgac gatttcgccg acgaacttca cccatggaac aggctcgccc
     3601 ggctgaccct tgtagggatc gtcgccacca aacgtgcggt gacccaccag gatttgtttc
     3661 ggtaatcagc tgatccacaa cggaattcgg gccgacatag tcagccaacc acgacatgtc
     3721 gccatcgggc ttgcgataaa gccgtcgagt gaggacgccg tggaatacag gagcttggtc
     3781 ataaaggtga tgaccactgg agtccaaacg attcatcgcc ggggctgcgg ggagatcgcc
     3841 aattcggccg cgaattcgat ctgcccgcag tttcgacgcg agcctgactg atttgccaaa
     3901 tatattcgcg accgtcaaat tcagctaaac gcggtggcgg agggatttga acccccggac
     3961 ggttttagcc gtctctcgct ttcaaggcga gtgcattagg ccgctctgcc acgccaccgc
     4021 cgacaagagt acggcgtgga tgagggcttg cgcgaagacc caacgagtca gacgcggccg
     4081 agcgccggga cggctagacg cggccgagct tcgccggcgg cgagccggac ttcagcgcga
     4141 cgctgattcg cctgcagttc tcgcccatgg cagcgatctg cggtcgagat aatctgccca
     4201 gaaaatgcgc gcgaaccccc tcgccatacg tcagcatcgc ctcggtcacc gcgacacgcc
     4261 cttcgtcggt gattgaggcc agcacgcccc ggccgtcatc agggctggca ccgcggcgca
     4321 ccaggccctg caattcca
//
LOCUS       DASRJA010000398         8857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3667076,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000398 DASRJA010000000
VERSION     DASRJA010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8857
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3667076"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFC01_17515"
     CDS             complement(<1..509)
                     /locus_tag="VFC01_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN81464.1"
                     /translation="MGIEYESIVDHPLADVFAWHTRRGAMTRLVPPWQPMTVVAETES
                     LADGRAVLGLPGGLRWVAQHDASAFDPPHRFVDELSSQGPLSWPARIVGRWRHTHEFS
                     EAPGGTRVYDHVDTAVPAAALRSTFVYRHRQLADDLAAHRAAGAQPMVVAVTGASGLV
                     GSALSAFLST"
     gene            640..1272
                     /locus_tag="VFC01_17520"
                     /pseudo
     CDS             640..1272
                     /locus_tag="VFC01_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
     gene            complement(1269..1577)
                     /locus_tag="VFC01_17525"
     CDS             complement(1269..1577)
                     /locus_tag="VFC01_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HZN81465.1"
                     /translation="MLIVALVLAVIGLAALVTAVVTSNELIAWVCIAASVIGVVLLIV
                     DALRERSKGEASEPEPEAEAEEPEYAEDYPEEAPVAHDDESDAETTAVKHEDEDSNKR
                     "
     gene            complement(1625..2455)
                     /locus_tag="VFC01_17530"
     CDS             complement(1625..2455)
                     /locus_tag="VFC01_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HZN81466.1"
                     /translation="MPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIE
                     ENADLRQRVAELDQELATAGSGGGGQSTQAIPLYEPTPAPEPAPQPVYDAPPQPVYDA
                     PPQPAAATEESHIKAAKVLSLAQDTADRLTGTAKAESDKMLADARAQADAMVSEARQT
                     AETTVAEARQRADALLADAQTRSEAQLRQAQEKADALQADAERKHSEIMGTINQQRTV
                     LEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSAAPVDSSANNDGGGFNQFNRGNN
                     "
     gene            complement(2673..2963)
                     /locus_tag="VFC01_17535"
     CDS             complement(2673..2963)
                     /locus_tag="VFC01_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HZN81467.1"
                     /translation="MSLFFEILGFALFVFWLLLIARVVVEFIRSFSRDWHPKGLTVVV
                     LEVIMTVTDPPVKLLRRLIPQLTIGAVRFDLSIMVLLLVAFIGMQLAFGAAA"
     gene            complement(3065..3694)
                     /locus_tag="VFC01_17540"
     CDS             complement(3065..3694)
                     /locus_tag="VFC01_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HZN81468.1"
                     /translation="MSTLHKVKAYFGMAPMEDYDDEYYEDDDRGSRGYSRRPRDDRFD
                     EDGYSRGYEDRDYDEPSGYRGGYRDEDRFEARLRGPREFDRPTPRFSALRGSTRGALA
                     MDPRRMAELFDAGSPLSKITTLRPKDYSEARTIGERFRDGTPVIMDLVSMDNADAKRL
                     VDFAAGLAFALRGSFDKVATKVFLLSPADIDVSAEERRRIAEAGFYAYQ"
     gene            complement(3749..4498)
                     /locus_tag="VFC01_17545"
     CDS             complement(3749..4498)
                     /locus_tag="VFC01_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZN81469.1"
                     /translation="MTALASAREQELADALAAVRTRLARAAEAAGRNENEIELLPITK
                     FFPASDVLILRRLGCLQFGESREQEASNKSAEVGAVLGAEPIRWHMVGRIQRNKARSI
                     SRWAYAAHSVDSARVIAALARAAAEELEDGGRAEPLRVYLQLSLDGDESRGGVDIGRE
                     EWVDELCAAIDAADGLEFVGLMAIPPLDTDPDEAFLRLQEEKERLQKSYEQPLGLSAG
                     MSSDLESAVKHGSTCVRVGTALLGQRPLTSP"
     gene            complement(4495..5202)
                     /gene="pgeF"
                     /locus_tag="VFC01_17550"
     CDS             complement(4495..5202)
                     /gene="pgeF"
                     /locus_tag="VFC01_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HZN81470.1"
                     /translation="MSVRIRRVTTTRAGGVSAPPFDTFNLGDHVGDDPAAVAANRKRL
                     TGALGLGDDAVVWMNQVHGDHVVEVDGPRNGAVDKTDALVTTTPRLALAVVTADCVPV
                     LMGDARAGVVAAVHAGRVGAQKGVVVRTVEVMLRAGARAKDISALLGPAVSGRNYEVP
                     EEMAAEVEAALPGSRTTTSRGTPGLDLRAGIATQLTALGITAIDIDPRCTVDDRNLFS
                     HRRDAPTGRLASLVWME"
     gene            complement(5225..6373)
                     /gene="ftsZ"
                     /locus_tag="VFC01_17555"
     CDS             complement(5225..6373)
                     /gene="ftsZ"
                     /locus_tag="VFC01_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZN81471.1"
                     /translation="MTPPHNYLAVIKVVGIGGGGVNAVNRMIEQGLKGVEFIAINTDA
                     QALLMSDADVKLDVGRDSTRGLGAGADPEVGRKAAEDAKDDIEELLRGADMVFVTAGE
                     GGGTGTGGAPVVATIARKLGALTVGVVTRPFSFEGKRRSNQAENGITSLRESCDTLIV
                     IPNDRLLQMGDAAVSLMDAFRSADEVLLNGVQGITDLITTPGLINVDFADVKGIMSGA
                     GTALMGIGSARGDGRALKAAEIAINSPLLEASMEGAQGVLMSIAGGSDLGLFEINEAA
                     SLVQEAAHAEANIIFGTVIDDSLGDEVRVTVIAAGFEATGPGRRPVVGAGAQAIAPGK
                     AGKVSTPLFEPADAASVPVHTNGATVSIGGDDGGISDDDVDVPPFMRH"
     gene            complement(6563..7456)
                     /locus_tag="VFC01_17560"
     CDS             complement(6563..7456)
                     /locus_tag="VFC01_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HZN81472.1"
                     /translation="MTEPTDENAGEDAPQEQTPPPAAAPDYEGPRRRARREREERRAA
                     EARATAIENARREAKRRVMGGPPEETKRLGRRTVRGLKVLMWSALVSVVVVGLGLLLY
                     FTPIMSARDVVVVGLGAVTQDEVIAAAAVAPGTPLLQVDTDAVAERVAAIRRVASARV
                     QRQYPSTLRVTVIERVPVVVKDYPDGPHLFDKDGVDFATAPPPAGVPYLDADNPGPSD
                     APTKAALQVMTALRPEVAGQVGRIAAPSVAAITLTLNDGRTVVWGTTDRTEEKALKLG
                     ALLTQPGRTYDVSSPDLPTVK"
     gene            complement(7453..>8857)
                     /gene="murC"
                     /locus_tag="VFC01_17565"
     CDS             complement(7453..>8857)
                     /gene="murC"
                     /locus_tag="VFC01_17565"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZN81473.1"
                     /translation="SGIARILLDRGGLVSGSDAKESRGVVALRARGASIRIGHDESSL
                     DLLPGGPTAIVTTHAAIPKTNPELVEARRRGIPVILRPAVLAKLMAGHTTLMVTGTHG
                     KTTTTSMLIVALQHSGFDPSFAVGGDLGEAGTNAHHGSGDCFVAEADESDGSLLEYTP
                     DVAVVTNIEADHLDFFGSAEAYGAVFDGFVERLKPGGALVVCTDDAGAAGLAERTAAL
                     GIRVLRYGSDPTQPLAGTLLDWEQQGTGAVAHIQLDGETHPRVMRLAVPGRHMALNAL
                     GALLAALDIGAPPDAVLDGFAGFEGVRRRFELVGTSNGVRVFDDYAHHPTEVRATLTA
                     VRAVAQNGDPGFSGRSIVVFQPHLYSRTKTFAREFGQALSEADEVFVLDVYAAREQPM
                     AGVSGAMIADHVTVPVTYVPDFSAVAERVAAAANPGDVVVTMGAGDVTMLGTEILAAL
                     RVKANRSAPGGPGAAFR"
BASE COUNT         1411 a         3040 c         2893 g         1513 t
ORIGIN      
        1 gtgcttagaa acgccgacag cgcggagccg accagacccg aagcaccggt gactgcgacc
       61 accatcggct gcgcacctgc agcacggtgt gcggcgaggt cgtcagcgag ctgcctatgc
      121 cgatagacga acgtcgagcg cagcgctgct gcaggcaccg cagtgtcgac gtggtcgtag
      181 acccgtgtgc cacccggcgc ctcgctgaac tcatgcgtgt gccgccaccg gccgacaatc
      241 cgcgccggcc acgacaacgg gccctgcgac gacaattcgt cgacgaatcg gtgtggcggg
      301 tcgaatgccg atgcatcgtg ctgggccacc cagcgcagtc cacccggcag gcccagcacc
      361 gctcgcccgt cggccagcga ctccgtctcc gccaccaccg tcatcggttg ccacggcggc
      421 accagccggg tcatcgctcc gcgccgggtg tgccatgcga acacgtcggc gagcggatgg
      481 tcgacgatgc tttcgtattc gatgcccatg tttgtgggta cccacgcgat accgaggtca
      541 gagaacaccg gtcgacgtac gctgaatatg tccccgatat ctaaaaagat cgcgatgagc
      601 ggatggagcg ggctcgcccg acgaaatgcg aggcgaacct tccgggaccg ccgagaagcc
      661 ggccaagtcc tggctgaaga gctggcgtcc taccgcggca aggacaacct gttggtgctt
      721 ggcctggcgc gaggcggcgt gcccgtcgga tgggaggtcg cgtcgtatct gcgggcgccg
      781 ctcgacgtat ttctggtgcg caagctcggg gtgccgcaat gggaggaact cgcgatgggc
      841 gccatcgcaa caggcggcgg cgtcgtggtc aacgacagct tggtgcgcaa cctcggcatc
      901 agcgacgaac agctgcaggc cgcgatcgac cgggaaaccg aggaacttca ccgtcgcgag
      961 ggcgcctatc gcggcgggcg tccaccagtg gacatcgcgg gtaacacggt gatcctcgtt
     1021 gacgacggga tcgccaccgg ggccagcatg ctcgccgccg tgcgtgcggt gcgggcggcc
     1081 cagcccgccc gggtggtggt cgcagtgccg gtcggaccgg aatcggcctg ccggcagctc
     1141 gccgaggaag ccgacgacgt cgtatgcgca acgatgccac ccggatttga agctgtcggc
     1201 caggtcttcg aggatttcca tcaggtcacc gacgacgaag tgcgcgaatt gctagctacg
     1261 ccaacggttt agcgcttgtt gctgtcctcg tcctcatgct taaccgcggt ggtttcggcg
     1321 tcgctctcgt cgtcgtgcgc gacgggggcc tcttcgggat agtcctcggc gtactccggc
     1381 tcctcggctt cggcttcagg ctcgggttct gacgcctcac ctttggaccg ttcccgcagc
     1441 gcgtcaacga tcagcagcac cacgccgatc acactggcag cgatgcagac ccaggcgatc
     1501 agctcattgc tggtgacgac ggcggtcacc agggccgcga ggccgatgac ggcaagcaca
     1561 agcgcaacga tcagcattga ccaaccctag gtggtagacg cagcgttttc aggcaacccg
     1621 attgctagtt gttaccccgg ttgaattggt tgaagccgcc accgtcgttg ttggcgctcg
     1681 agtcgaccgg tgcggcggag ccgcgctggc caagctcttc gagctgggac tccagatagg
     1741 tcttgagccg ggtgcggtat tcgcgctcga acgtgcgcag ctgttccagc cgcccttcca
     1801 gcacggtgcg ctgctggttg atggtgccca tgatctcgga atgcttgcgc tcggcatcgg
     1861 cctgtagggc gtcggccttc tcctgcgcct ggcgcagctg cgcctccgac cgggtctggg
     1921 cgtcggccag cagtgcgtcg gcacgctgac gggcctcagc gaccgtggtc tcggcggtct
     1981 gccgggcctc gctcaccatc gcgtcggcct gggcgcgggc atcggcgagc atcttgtccg
     2041 actcggcctt ggccgtgccg gtcagccggt ccgcggtgtc ctgggcgagg ctcagcacct
     2101 tcgcggcctt gatgtggctc tcctcggtcg ctgcggcggg ctgtggcggt gcgtcgtaga
     2161 cgggctgcgg cggcgcgtcg tagacgggct gcggcgcagg ctcgggcgcg ggcgtcggct
     2221 cgtacagcgg aatggcctga gtcgactgcc cgccaccgcc ggagccggcc gtggccagtt
     2281 cctggtccag ctccgcgacc cgctgacgaa ggtcggcgtt ctcttcgatg agccgggtca
     2341 gttcgttctc aaccagatcg agaaaggcgt cgacctcgtc ctcgttatag ccgcgcttgc
     2401 cgatcggtgg cttgctgaac gcgacgttgt ggacgtcggc tggtgtgagc ggcattgtct
     2461 gcccccttgg agttctggac cgtcaaccga tctcaaagtg tagagcctta ctggaacgta
     2521 actggcgccc atcctgtcac accagacccg gcggttgcag gagaggatga attttaagag
     2581 cgaatttcaa tcttcttact cggaatatcg accccggcaa acctgcgtgg gctaccgcag
     2641 cgatcgccaa agacggggcg gcaggccagt tctcaggccg ctgcgccaaa tgccagctgc
     2701 atgccgatga aggcgaccaa cagcagcacc atgatcgaca gatcgaatcg gaccgcgcct
     2761 atcgtgagct gcggaatgag ccgtcgaagc aacttcaccg gcggatcggt gacggtcatg
     2821 atcacctcga ggaccaccac cgtcagcccc ttgggatgcc agtcgcgcga aaacgaccgg
     2881 atgaactcga cgacgacccg cgcgatcagc agcagccaga agacgaacag cgcgaaaccc
     2941 aggatttcga agaagagcga caacgacagc cgtcctcact acgggcagaa gtgtgacttt
     3001 gacaacagcg ctcgctgctg gtcagcgcca agcgcgacgc cagcctacct gtccgggctg
     3061 agatctactg ataggcgtag aagccggcct cggcgatacg cctgcgctcc tcggcgctca
     3121 cgtcgatgtc ggccggagag agcaggaaca ctttggtggc gaccttgtca aaggatccgc
     3181 gcagcgcgaa cgccagcccg gcggcgaagt cgaccagccg cttggcgtcg gcgttgtcca
     3241 tcgacaccag gtccatgatc accggggtgc cgtcgcggaa gcgttcaccg atggtgcgcg
     3301 cctcgctgta gtccttcggg cgcagcgtgg tgatcttcga cagcgggctg cctgcgtcaa
     3361 agagctctgc catccggcgt gggtccatcg cgagggcgcc tcgcgtcgag ccgcgcaggg
     3421 cgctgaaccg gggtgtcggc cggtcgaatt cccgcgggcc ccgcagtctg gcctcaaacc
     3481 ggtcctcgtc acggtatccg ccgcggtagc ccgagggttc gtcgtagtca cgatcctcgt
     3541 agccgcggct gtaaccgtcc tcatcgaacc ggtcgtcgcg cggacgacgc gagtaaccgc
     3601 gcgaaccgcg gtcgtcgtcc tcgtagtact cgtcgtcgta gtcctccatc ggcgccatac
     3661 cgaagtaggc cttgaccttg tgcagtgtgc tcatcggtgg acccttctcg cgtggtgtct
     3721 gtgatgaaga tgtgactaga gtgactactt agggtgacgt tagaggacgt tgccccaata
     3781 gcgcggtacc gacacgcaca cacgtcgaac cgtgtttgac cgcgctctcg aggtcgctgg
     3841 acatgcccgc ggacagtccg aggggctgtt cgtaggactt ctgcagccgc tccttttctt
     3901 cttgcaatcg caagaaggct tcatccgggt cggtgtccag cggcgggatg gccatcagcc
     3961 cgacgaactc caacccatcc gcggcgtcga tggcggcgca cagctcgtcc acccactctt
     4021 cacggccgat atccacccca ccccgcgact cgtcgccgtc gaggctgagc tggaggtaga
     4081 cccgtagcgg ctcggcccta ccgccgtcct ccagctcctc ggcggccgcg cgggccagcg
     4141 cggcgatcac cctggcgctg tcgaccgagt gagccgcgta tgcccagcgc gagatcgacc
     4201 gcgccttgtt gcgctgaatc cgcccgacca tgtgccagcg aatcggctcg gcacccaata
     4261 cggcaccgac ttcggcactt ttattcgacg cttcctgttc gcgggattcg ccaaattgca
     4321 gacaccctaa acgccgcaaa atcagcacat cgctagccgg aaagaatttc gtaatgggca
     4381 gcaattcaat ttcattttca ttgcgtccgg cggcctcggc cgcccgggcc aggcgggtgc
     4441 gaacggccgc gagcgcgtcg gccaattcct gttcacgggc gctcgccaag gcggtcattc
     4501 catccacacc aacgacgcca gtcgtccggt gggggcatcg cgtcggtggc tgaacaggtt
     4561 gcggtcgtcc accgtgcagc ggggatcgat gtcgatggcc gtgatgccca aagccgttag
     4621 ctgcgtggcg attccggccc gcaggtccaa cccgggagtg ccccgcgacg ttgtggtgcg
     4681 actgcccggc agcgctgcct cgacctccgc ggccatctct tccggcactt cataattgcg
     4741 tccgctgacc gcggggccca acagcgccga aatgtccttg gcgcgtgcgc cggcccgcag
     4801 catcacctcc acggtcctga cgacgacgcc cttctgcgcg ccgacccggc ccgcgtgaac
     4861 cgcggccacc accccggcgc gggcgtcgcc cattaaaact ggaacacaat ctgcggttac
     4921 caccgccaaa gccaatcgtg gcgtggtagt gaccaatgcg tcggttttgt cgacggcgcc
     4981 gttgcgcggt ccgtcaactt ctaccacgtg atcgccgtgc acctggttca tccacaccac
     5041 cgcgtcatcg cccaacccga gcgcgcccgt gagccgcttg cggttggcgg cgaccgccgc
     5101 cggatcgtcc ccgacatggt cgccgagatt gaaggtgtcg aacggcggtg ccgaaacgcc
     5161 gccggcgcgg gtggtggtca cgcgacggat acgaacgctc actcgcccag tatccggacg
     5221 cggttcagtg gcgcataaag ggcggcacgt cgacgtcatc atcggaaatg ccgccgtcgt
     5281 cgccaccgat gcttaccgtc gcgccgttgg tgtgcaccgg cacgctggcc gcgtcggcgg
     5341 gctcgaacag cggcgtgctg accttgccgg cctttcctgg cgcgatcgcc tgagccccgg
     5401 ccccgacgac cggccttcgg cccggtccgg tggcctcgaa gccggcggcg atcaccgtga
     5461 cgcggacctc gtcgcccagc gagtcgtcga tgaccgtgcc gaagatgatg ttggcctccg
     5521 cgtgggcggc ctcctgcacc agcgaggcgg cctcgttgat ctcgaacaga ccgagatcgc
     5581 tgccacccgc gatcgacatc agaacgccct gggcgccctc catcgacgcc tccagcagag
     5641 gcgaattgat cgcgatctcg gcggccttga gcgcccggcc gtccccacgc gccgagccga
     5701 tgcccatcag cgccgtgccc gccccgctca tgatgccctt gacgtcggcg aagtcgacgt
     5761 tgatcaagcc cggcgtggtg atcaggtccg tgatgccctg cacaccgttg agcaacacct
     5821 cgtcggcgct gcggaacgcg tccatcaacg acaccgcggc gtcgcccatc tgcagcagcc
     5881 gatcgttcgg aatgacgatt agcgtgtcgc agctttcgcg cagcgaggtg atgccgttct
     5941 cggcctgatt gcttcgccgc ttgccctcaa acgagaacgg ccgcgtcacc acgccgacgg
     6001 tcagggcacc gagcttgcgg gcaatggtcg cgacgaccgg cgcgccaccg gtgccggtgc
     6061 caccgccctc gcccgcggtg acgaacacca tgtcggcccc gcgcagcagt tcctcgatgt
     6121 cgtccttggc gtcctccgca gccttgcgcc cgacttcggg gtcggcgccc gcgcccagac
     6181 cacgggtgga gtcgcgtccg acgtcgagct tgacgtcggc gtcgctcatc aacaacgcct
     6241 gggcgtcggt gttgatggcg atgaactcga cgcccttgag gccctgctcg atcatccggt
     6301 tgacggcgtt gacgccgcca ccgccgatgc cgaccacttt gatgacggcg aggtagttat
     6361 gcgggggggt catgatcgcc ttcctccctg gttgcctttc gtcgcggcga acgccttggc
     6421 aaactctcaa cctcaaccag agggttatag ttatgtcaag ttgttcctcg caaccagaac
     6481 cgtagggcga ggtcgcgagg tatccatgca ggcgcgccga tgggcgtgtc gagaatttcc
     6541 gcgcaacgtt gggccctcgg gactatttga ctgtcggcag gtccgggctg gacacgtcgt
     6601 aggtccttcc gggctgggtg agcagagcgc cgagtttgag cgccttctct tcggtgcggt
     6661 ccgttgttcc ccacaccacc gtgcgcccgt cattcagggt cagggtgatg gcggcgacgg
     6721 acggcgcggc gatgcggccg acctgtccgg cgacctcggg gcgcagcgcc gtcatcacct
     6781 gcagcgccgc cttggtcggc gcgtcgctgg gtccggggtt gtcggcgtcg aggtagggca
     6841 cgcccgcggg cggcggcgcc gtcgcgaagt ccacaccgtc cttgtcgaac agatgcgggc
     6901 cgtccgggta gtccttgacc acgactggca cccgctcgat gacagtgacg cgcagcgtcg
     6961 acgggtactg acgctggacc cgcgcgctgg ccacccgtcg aatcgccgcg acacgttcgg
     7021 cgaccgcatc ggtgtcgacc tggagcaggg gcgtgcccgg tgcgactgcg gcggcggcga
     7081 tgacctcatc ctgcgtcacc gcgcccaggc cgaccaccac gacgtcgcgc gctgacatga
     7141 tcggcgtgaa gtacagcagc agcccaagcc cgaccaccac gacgctcacc agcgcggacc
     7201 acatcagcac cttcaggccc cgaaccgtcc ggcggccaag ccttttcgtc tcttcgggcg
     7261 gcccgcccat gacgcgccgc ttggcctctc ggcgagcatt ctcgatcgcg gtggcccggg
     7321 cctcggcggc acggcgctcc tcacgttccc gccgtgcccg ccgacgcgga ccctcgtagt
     7381 cgggagcggc cgcgggcggc ggtgtctgtt cctgcggggc gtcttcgccg gcgttctcgt
     7441 cggtcggctc ggtcatcgga acgccgctcc cggcccgccc ggtgcgctgc ggttggcctt
     7501 caccctcagc gccgcaagga tttccgtacc cagcatggtg acgtcgcctg cccccatcgt
     7561 caccacgacg tcgcccgggt tggcggccgc ggccacccgc tctgccaccg ccgaaaagtc
     7621 gggaacgtac gtcaccggga cggtgacgtg gtcggcgatc atcgccccac tcacgcctgc
     7681 catcggctgt tcgcgcgcgg catagacgtc gaggacgaac acctcgtcgg cttcgcttag
     7741 cgcctgcccg aattcgcgtg cgaaagtttt tgtccgcgaa tacaaatggg gttggaacac
     7801 gacgatcgac cgaccggaaa agccggggtc gccgttctgg gccactgcgc gcaccgcggt
     7861 cagcgtggcg cgcacttccg tcggatggtg ggcgtaatca tcgaacacgc gcacgccgtt
     7921 ggaggtgccg accaattcaa agcgccgccg caccccctcg aaaccggcga agccgtcgag
     7981 cacggcgtcg ggcggcgcgc cgatgtcgag cgccgccagc agcgcgccga gtgcgttgag
     8041 cgccatgtgc ctgccgggca ccgcgagccg catcacccgc ggatgcgttt cgccgtccag
     8101 ttggatgtgc gcgaccgcac cggtgccctg ttgttcccag tccagcaggg tgcccgccag
     8161 cggctgtgtc gggtcgctgc cgtatcgcag cacccgaatg cccagtgctg cggtgcgttc
     8221 ggccagcccc gcagcgccgg cgtcgtcggt gcaaacgacc agcgcaccac cgggtttcag
     8281 ccgctcgacg aacccgtcga acaccgcgcc gtacgcctcg gcgctgccga agaaatccag
     8341 atgatcggcc tcgatgttgg tcaccaccgc gacatccggg gtgtactcca gcagcgagcc
     8401 gtcactctcg tccgcctcgg cgacgaaaca atccccgctg ccgtggtgcg cattggtgcc
     8461 cgcctcgccg agatcgccgc ccaccgcgaa cgacgggtca aacccgctgt gctgcaacgc
     8521 cacgatgagc atcgaggtag tggtggtctt gccgtgggtg ccggtgacca tcagcgtggt
     8581 gtgtccggcc atgagcttgg ccagcaccgc cggtcgcagt atcaccggga tgccgcggcg
     8641 acgcgcctcg accagttcgg ggttggtctt gggaatggcg gcgtgcgtgg tgactatggc
     8701 cgtcgggccg ccgggcagca ggtccagcga cgactcgtcg tgcccgatcc ggatcgacgc
     8761 gccgcgggcg cgcagcgcca ccacgccgcg cgattccttg gcgtccgaac ccgataccag
     8821 gccaccgcgg tccagcagga tgcgagcgat acccgac
//
LOCUS       DASRJA010000399         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1129011,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000399 DASRJA010000000
VERSION     DASRJA010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129011"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFC01_17570"
     CDS             complement(<1..234)
                     /locus_tag="VFC01_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81474.1"
                     /translation="MTLEEAFTIGVIAVLATAATAAIYLGLLNWMGAFYVVRCVACHH
                     LTFSSSNRSQESCPHCRHPAFMHPIYSLRHSGAR"
     gene            445..2334
                     /gene="typA"
                     /locus_tag="VFC01_17575"
     CDS             445..2334
                     /gene="typA"
                     /locus_tag="VFC01_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZN81475.1"
                     /translation="MDFRNVAIVAHVDHGKTTLVDAMLRQSGALHHRGDDAIERLMDS
                     GDLEKEKGITILAKNTAVHRKNGDGSVTVINVIDTPGHADFGGEVERGLSMVDGVLLL
                     VDASEGPLPQTRFVLRKALAAHLPVILVVNKTDRPDARISEVVSESHDLLLDVASDLD
                     DEAQKAAEDALGLPTLYASGRAGIASTTEPANGENPEGENLDPLFDVLLEHIPPPQGD
                     PEAPLQALVTNLDASAFLGRLALIRIYKGRIRKGQQVAWMRDVDGHPVITNAKITELL
                     VTEGVERTATDEAIAGDIVAVAGIPEIMIGDTLADPDHAHALPRITVDEPAISVTIGT
                     NTSPLAGKVSGHKLTARMVKSRLDSELVGNVSIKVVDIDRPDAWEVQGRGELALAVLV
                     EQMRREGFELTVGKPQVVTRTIDGKLHEPFEAMTIDCPDEFVGAITQLMAGRKGRMEE
                     MTNHAAGWVRMDFIVPSRGLIGFRTDFLTLTRGTGIANAVFDGYRPWAGEIRARHTGS
                     LVSDRAGSITPFAMIQLSDRGQFFVEPGEDTYEGQVVGINPRAEDLDVNVTREKKLTN
                     MRSSTADVMETLARPLELGLEQAMEFCAEDECVEVTPEMIRVRKVELDATTRARSRSR
                     AKARG"
     gene            complement(2364..>2595)
                     /locus_tag="VFC01_17580"
     CDS             complement(2364..>2595)
                     /locus_tag="VFC01_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007237985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty-acid--CoA ligase"
                     /protein_id="HZN81476.1"
                     /translation="SVVLVEHLSIPTTSSGKIQRGQCRQRFLTGDIAAVARWQAPSAP
                     DDLTKAKTLEVAVTAAELLKALARQQQASHQS"
BASE COUNT          476 a          870 c          856 g          393 t
ORIGIN      
        1 ccgcgcgccg gagtggcgca gcgagtagat cggatgcatg aaagccgggt gacggcagtg
       61 cgggcaggac tcttgcgacc ggttcgacga cgagaacgtc agatgatggc aggccacgca
      121 ccggacgacg tagaaggcgc ccatccaatt gagcagcccg agatagatgg ccgcggtcgc
      181 agcggtcgcc agaacagcga tgactccgat ggtgaaagcc tcttcgagcg tcatatgtgt
      241 ccacctccga ccactgttga cgggcgattg ggacgctacg cgcggaagct gtgagttgcc
      301 tgtccgaagc cggcgtcgaa actgcgccca gatcgtgatt ccttgagaat tcgcgatctc
      361 cccgcagact gggcgcaatc ggcgtgcagt gacgggcgtc tctcgttttc gtgtttggtc
      421 gaccgcactg ggagaatcac cagcgtggat tttcgtaacg tcgccatcgt ggcacacgtc
      481 gaccatggga agacgaccct ggtcgacgcg atgctgcgcc agtcaggcgc cctgcaccat
      541 cgcggcgacg acgccattga acgcctgatg gactccggtg acctggagaa ggaaaagggc
      601 atcacgatcc tggccaagaa caccgccgtg catcggaaga acggcgacgg ctcggtgacc
      661 gtcatcaacg tgatcgatac ccccgggcac gccgacttcg gcggcgaggt cgaacgcggg
      721 ctgtcgatgg tcgacggggt gctgctgctc gtcgacgcct ccgaggggcc gttgccgcag
      781 acccgattcg tgctgcgcaa agcgctcgcc gcccatctgc ccgtcatcct ggtcgtcaac
      841 aagaccgacc ggcccgacgc gcgcatctcc gaggtcgtct cagaaagcca cgacctgctg
      901 ctcgacgtgg cctccgacct cgacgatgag gcgcagaagg ccgccgagga cgcactcgga
      961 ctgccgacgc tctacgcgtc ggggcgcgca ggcatcgcca gcaccaccga accggccaac
     1021 ggcgagaatc ccgaagggga gaacctcgac ccgctgttcg acgtgctgct cgaacacatc
     1081 ccgccaccgc agggggatcc agaggcgccg ctgcaggcgc tcgtcaccaa cctcgacgcg
     1141 tcggccttcc tgggccgcct cgcgctgatc cgcatctaca agggccggat ccgcaagggc
     1201 cagcaggtcg cctggatgcg cgacgtggac ggtcatccgg tcatcacgaa cgccaagatc
     1261 accgaactcc tggtcaccga gggcgtcgag cgcaccgcga ccgacgaggc catcgccggc
     1321 gacattgtcg ccgtcgcggg catccccgag atcatgatcg gcgacaccct cgccgatccg
     1381 gaccacgcgc acgcgctgcc gcgtatcacc gtcgacgagc ccgccatctc ggtcaccatc
     1441 ggcaccaaca cctcgccgct ggcgggcaag gtgtccggac acaagctgac cgcccggatg
     1501 gtgaagtcgc ggctggactc cgagctcgtc ggcaacgtgt ccatcaaggt cgtcgacatc
     1561 gatcggccgg acgcttggga ggtgcaaggc cgtggcgagc tggcgctggc cgtcctcgtc
     1621 gagcagatgc gccgcgaagg cttcgagctg acggtcggca agccccaggt ggtcacccgg
     1681 accatcgacg gcaagctgca cgaaccgttc gaggcgatga cgatcgactg ccccgacgag
     1741 ttcgtcggcg ccatcacgca gctgatggcc ggccgcaagg gccgcatgga ggagatgacc
     1801 aatcacgccg cgggctgggt ccggatggac ttcatcgtgc ccagccgtgg actgatcggc
     1861 ttccggaccg acttcctgac actgacgcgg ggcaccggca tcgccaacgc ggtgttcgac
     1921 ggctaccggc cgtgggcggg ggagatccgg gcccgccaca ccgggtcgct ggtgtcggac
     1981 agggccggct ccatcacacc gttcgccatg atccagctgt ccgaccgcgg gcagttcttc
     2041 gtggagcccg gtgaggacac ctatgagggt caggtcgtcg gcatcaaccc ccgggccgag
     2101 gacctcgacg tcaacgtgac acgtgagaag aagctgacca acatgcgctc atcgacggcc
     2161 gacgtgatgg agacgctggc ccgtccgctg gagctgggcc tcgaacaggc gatggagttc
     2221 tgcgccgaag acgagtgcgt ggaggtgacg ccagagatga tccgggtgcg caaggtcgaa
     2281 ctcgacgcaa ccactcgggc ccgcagccgc tcacgggcca aggcccgcgg ctagctccgc
     2341 cggcaccgtc acctgcgcga acttcagctc tgatgggagg cctgctgctg gcgcgccaat
     2401 gctttcagga gctcggccgc cgtgaccgcc acctccagtg ttttggcttt ggtgaggtca
     2461 tcgggcgccg acggtgcttg ccatcgcgcc accgccgcaa tgtcgccggt gaggaaccgc
     2521 tgccggcact ggcctcgctg aatcttgcca ctcgaggtcg tcggaatcga caaatgctcc
     2581 accaagacca cggag
//
LOCUS       DASRJA010000400         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1164640,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000400 DASRJA010000000
VERSION     DASRJA010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164640"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VFC01_17585"
     CDS             complement(<1..1024)
                     /locus_tag="VFC01_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016882308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN81477.1"
                     /translation="MTSYQWNPTPDYIEGANVTRLARAHGLAGIDELRARSVEDTPWY
                     WDAVATDLGLRFRTPYSRVLDLSNGIEHPDWFVDGTLNIVDSCLLRWRDTTPRTIAIV
                     HEDEAGQIRELTFSELATEVARAAHGLRRLGIGPGDAVAIYLPMIPEAVVACYAVAAL
                     GAVLVPLFSGFASSAIASRLSDAEAKAIIVADVTTRRGRALPLLPQVSAALSQCPSVQ
                     HVIVVPNGSEGPPEYVGAQMVLWSQLTYRTEPLAPAEVDTMHPLVLAYTSGTTGKPKG
                     AVHTHAGFLVKTASEIAYSFDMKPGGVFCWVTDMGWIMGPLSVFGAHANGATLLLYEG
                     SPDRPDS"
     gene            complement(1040..2227)
                     /locus_tag="VFC01_17590"
     CDS             complement(1040..2227)
                     /locus_tag="VFC01_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007299644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN81478.1"
                     /translation="MELKDSAVEAEFRAKARAWLEETIPTLGGAEPPVLEDKLDYWRT
                     WQRLLYDAGYAGLSWPKQYGGGGADAKLRAVFNEELDRAGAPERLNIVGEDFAGPTII
                     QFGTPAQQERYLEPILTGNEIWCQLFSEPEAGSDLASLRTSATKVDGGWRIQGQKIWT
                     SRAQLAAHAILLARTGGGPRHKGITYFLLPMDSPGVDVRPLAHMLGEAEFNEVFLDDV
                     FIPDDGVLGEVDGGWRVTMATLAFERVAIATGRVNTTGAVNDLIREIADRTTDDGGPL
                     GADPLVRQRIADLYGRALVHYLIGQRVISGAAGSGPPGPVTSIGKLYFCPLVEDIADF
                     GLELDEMSGQFALAEKDIASSADRQRWLRLAYQARGTAIAGGSTFIQRNIVAERILGM
                     PKN"
     gene            complement(2227..>2549)
                     /locus_tag="VFC01_17595"
     CDS             complement(2227..>2549)
                     /locus_tag="VFC01_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HZN81479.1"
                     /translation="EIEDNLANHPDLLASSCVGMPDERLGERVCCYVVAAQGHTTPTV
                     EDVRNFLLSRGMPVQKTPERVVAVDALPMTATGKVLKQELRKDIEQRLEAEAASAHEV
                     TSHR"
BASE COUNT          438 a          813 c          828 g          470 t
ORIGIN      
        1 tgctgtcggg tctgtccggt gagccttcgt agagcagcag tgtcgcgccg ttcgcatggg
       61 cgccgaaaac cgacaggggg cccattatcc agcccatatc cgtgacccag cagaacacgc
      121 cgccgggttt catatcgaac gagtaggcga tttcgctggc tgtcttcacc agaaatccgg
      181 cgtgggtgtg caccgcacct ttcggtttgc cggtggtgcc cgacgtgtac gccagcacca
      241 gcgggtgcat cgtatcgacc tcagcgggcg ccaacggctc cgtgcggtag gtcaattgtg
      301 accacaaaac catctgtgcg ccaacgtatt cgggtggtcc ttcggagccg ttgggcacca
      361 caatcacgtg ctgcacgcta ggacattgcg acagcgcagc actgacttgc ggaagtagcg
      421 gtagcgcgcg tccccgccgt gtggtgacgt cggccacgat gatcgccttg gcctcggcgt
      481 cggagagtcg ggacgcgatc gccgaggacg cgaacccgga gaacagcggt accagaactg
      541 ctcccagtgc agccacggca tagcacgcta cgaccgcttc ggggatcatc ggcaggtaga
      601 tagccacagc atcgcccgga ccgattccca acctgcgcaa gccatgtgcc gcccgggcaa
      661 cctcggtggc cagctcgctg aatgtgagtt cacggatctg gccggcttcg tcttcgtgga
      721 cgatggcaat ggtgcgaggc gttgtatcgc gccagcggag cagacacgag tcgacgatgt
      781 taagcgttcc gtcgacgaac cagtcgggat gttcaatccc gttactaaga tccaagactc
      841 gactgtaggg cgtgcggaat cgtagcccga gatcggtagc caccgcatcc caataccaag
      901 gcgtgtcctc aacagagcgc gcgcgcagct catcgatgcc ggccaggccg tgagcacgag
      961 ccagccgcgt cacgttggca ccctcgatgt agtcgggagt cggattccac tggtaacttg
     1021 tcaatttgac tccgcgatct cagttcttgg gcatgccgag gatgcgctcg gcgacgatgt
     1081 tgcgctggat gaacgtcgac cctccggcga tggcggtgcc gcgagcctgg tacgccagtc
     1141 gcagccatcg ctggcggtcg gcagacgacg cgatgtcctt ctcggcgagt gcgaattgtc
     1201 cagacatctc gtcgagctcc aagccgaaat cggcaatgtc ctcgaccagg gggcagaagt
     1261 acagcttgcc gattgaggtc accggcccgg gtggtccgct gcctgcagca ccggagataa
     1321 cccgctgccc gatcagatag tgcacgaggg cgcgtccgta cagatcggcg atccgttgcc
     1381 gcacaagcgg gtcggcgccc agcgggccac cgtcgtcggt cgtgcggtcg gcgatttcgc
     1441 ggatcagatc gttgaccgcg ccggtggtgt tcacgcggcc ggttgcgatc gcgacccgtt
     1501 cgaacgccaa ggtggccatc gtgacgcgcc agccgccgtc gacttcgccg aggacgccgt
     1561 cgtcggggat gaacacgtcg tcgaggaaca cctcgttgaa ttcggcctcg cccagcatgt
     1621 gtgccagcgg gcggacgtcg acaccgggac tatccatcgg cagtaggaag taggtgattc
     1681 ccttatgtcg ggggccgccg ccggtgcggg caagcaggat ggcgtgggcc gccagttggg
     1741 cgcggctggt ccagatcttc tggccttgga tgcgccagcc accgtccacc ttggtggccg
     1801 atgtacgcaa cgaggccaga tccgatcccg cttccggctc ggagaataac tggcaccaga
     1861 tctcgttacc ggtcaagatc ggttcgaggt aacgctcctg ctgggcgggc gtgccgaact
     1921 ggatgatcgt cggcccggcg aaatcctcac cgacgatgtt cagccgctcc ggcgcgccgg
     1981 cccggtctag ttcctcgttg aagacggcgc gcaacttcgc gtcagcgccg ccaccgccgt
     2041 attgcttcgg ccaggacagg ccggcgtagc cggcgtcgta caacagccgt tgccaggtcc
     2101 gccaatagtc gagcttgtct tctagcacag gcggctctgc accaccgagc gtcgggatcg
     2161 tctcctcgag ccacgcgcgt gccttggccc ggaactcggc ttcgacggcg ctgtctttca
     2221 actccatcag cggtggctcg tcacttcgtg cgcgctggcg gcttcggcct cgaggcgttg
     2281 ttcgatgtcc ttgcgcaact cttgtttgag gaccttgcca gttgcggtca tcggcagggc
     2341 gtcgacggcg acgacgcgct cgggtgtctt ctgaaccggc atgcctcgcg acagaaggaa
     2401 attccgcacg tcctcgacgg tcggggtggt gtggccctgc gcggcgacca cgtagcagca
     2461 cacccgctca ccgagccgct cgtcgggcat gcccacacac gacgacgcca gcaggtccgg
     2521 atggttggcg aggttgtcct cgatttcgc
//
LOCUS       DASRJA010000401         6692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_64437,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000401 DASRJA010000000
VERSION     DASRJA010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64437"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFC01_17600"
     CDS             <1..272
                     /locus_tag="VFC01_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN81480.1"
                     /translation="NWKGDPNATLSGQDIPQLPPGSPPPGPPPTGNPPPAAAAPPPPI
                     AAAEYDPATGTYVGPDGHVYTQADLAQDTKAPKTWQSMLTSPNGN"
     gene            complement(545..2080)
                     /locus_tag="VFC01_17605"
     CDS             complement(545..2080)
                     /locus_tag="VFC01_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN81481.1"
                     /translation="MPETSYAKCGDLSLAYQVFGDGPVELVFSGSYVSHVELYWTVPE
                     FVVLFERLSTFCRVVLFDYAGVGLSDPVPHVRTMDDRAAEIEAVMDAVGFERAALFGP
                     SAGGPASIFFAATRPQRTRALILNGTAAYSVADSWDDLDRDPDELRARLVRELGEVYT
                     PSVQQLARWQAWGRAVVSAWGSGEASKVLLPSMSIRLLAMLERMCASPGMVRAGLEAM
                     FRIDLRPILPTLTVPTLVIHARDELVPVQFGRDMADHIPGARLLEVEGRDHAWLADPD
                     RIFTVIEEFLTGSHAAPAQSHRALRTVLFTDIVASTHQAAATGDERWRAVLQRFGELA
                     AELTERFGGTVIKSTGDGHLMTFEGPTQAIRCAEALRVESETLGLEIRAGIHTGECEL
                     LDNDIGGIAVHIAARILGKAGAGEILVSSTVRDLVVGSGTGFEDRGSGELRGVPGTWQ
                     LLAVDRLGPPAGSAEAELVSMPTPHRRTTMSRSDRAVEVIARRTPWILRGMARLAPTG
                     GRR"
     gene            2318..2704
                     /locus_tag="VFC01_17610"
     CDS             2318..2704
                     /locus_tag="VFC01_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81482.1"
                     /translation="MLWHQSHWSELNTPQLGSHVYRTLHKAIYEVKKRDDIEIKPWNP
                     DKHKVANVLEATEALAHLDAEIHPPEWIGLHSAAETAAAQMISCENALLDPVDPADPS
                     AHTDAVQCRVRAVRLRRERAGTCRVA"
     gene            2805..3191
                     /locus_tag="VFC01_17615"
     CDS             2805..3191
                     /locus_tag="VFC01_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN81483.1"
                     /translation="MTDLRDRIIKAFQKRVGNPLTRRLPTQTLLETTGRKSRQPRRTP
                     LGGSRVGDQFWFVSEFGEKSQYVQNIKANPNVRVRLGGRWHNGTAHLVPDDDPRARLH
                     SLPQLNSLAVRAVGTNLLTIRVDLTD"
     gene            3902..4426
                     /locus_tag="VFC01_17620"
     CDS             3902..4426
                     /locus_tag="VFC01_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81484.1"
                     /translation="MSAKTDIDQLVLLADDALNSRVEAAAARIETRIERAMDGGRDLT
                     QREYELTQQDRDELVALRDATEIRSVRGDQSDRIEQETRDRAQLIGGVIETRGNLSPL
                     FPSAENLARLEEARWQQSTLTLVKERAAIKTTDAGTAREYAPSGLLAPRGGCLRPTRR
                     GHPAGITSRVLYGL"
     gene            complement(4653..>6692)
                     /locus_tag="VFC01_17625"
     CDS             complement(4653..>6692)
                     /locus_tag="VFC01_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN81485.1"
                     /translation="DELVALLKSLPYVADVKSAWTVPPPAARALISEDGKTGLVVAGI
                     TGGETGAQQHAKDLLGSLPRFDGVTVKPGDEVITYAQIIDQTKKDLLVMEAIAFPLSF
                     IVLVWVFGGLLAAGLPLAVGIFAILGSMAVMRAITFATEVSVFALNLILAMGLALAVD
                     YTLLIVSRFRDELADGAGRDQALVRTMTTAGRTVLFSATTVALAMIAMVIFPMYFLKS
                     FAYAGIAVVAFAAAAAVVVTPAALVLLGDRLDALDVRRVVRGVLGRPEPVHRPVDETF
                     WYGWTKAIMGPAIPIGVAVIAVLLVLGAPFLGVKWGYPDDRVLPVSASARQVGDELRS
                     GFSVNSLTNVIVVIPDTGAVTAAELGFYAAQLSRVPDVSAVSSPAGTYASGVLGGPPS
                     APTGWKDGSAYLTVNSTAPLYSQASETQLDRLHAIATPGNATVQMTGWAQINRDSSHA
                     VTSRLPIVLSVMATITFLLLFLLTGSVILPLKALALNMLSLTAAFGALVWIFQDGHLD
                     GFGTTATGTLIASVPVLLFCLAFGLSMDYEVFLISRIGEYWQASGKTRADNTESVALG
                     LARTGRVVTAAALLMAVTFSSLAAAKVSLMCMFGVGVTLAVLVDATLVRILLVPVFMR
                     VLGRVNWWAPAPLVRLHRHIGISEYVELPDLSRSGSQHTREARAIEVSGSPLHER"
BASE COUNT         1323 a         2251 c         2088 g         1030 t
ORIGIN      
        1 acaactggaa gggcgacccc aacgccaccc tgtcgggcca agacatcccc cagttgccac
       61 cgggctcgcc gccgccgggc ccacctccga ccggtaaccc gccacctgcc gccgcggcgc
      121 cgccaccacc gatcgcagcc gccgagtacg acccggcgac gggcacctac gtcgggcccg
      181 acggacatgt ctacacccaa gcagacctcg cccaagacac gaaggcacct aagacatggc
      241 agtcgatgtt gacgtccccg aacgggaact gagcgcccgc ggggagctgt ccgaagacga
      301 ctcctcacac ggcgatgaca agttcaccga gcagcgggac actcggaagc ggcgtcaccg
      361 aacagtactg cgatagagac ggcaggtggc ggcgccttgt gcaagcggtg gtagcgactt
      421 tgtgaaggac gttttagctt ccgagaccga ggccagtctg ggccgcccgg cttttacgtt
      481 gaaacggtcg ctatccctca cggtgtgatg gcgtaaaccg cgttgcggct caacgtatct
      541 ctgttcatct acggccacca gtaggggcga ggcgggccat cccgcgcaga atccacggtg
      601 tccgcctggc gatcacctcg acagcgcggt ccgagcggct catcgtggtg cgacggtggg
      661 gggtaggcat tgataccagc tctgcctcgg ccgacccggc cggcgggccg aggcgatcga
      721 ccgccaagag ttgccaggtg ccgggcacgc cacgcagctc gccgctgcca cgatcctcga
      781 agcccgtgcc cgagccgacg accaggtcac gcacggtgct cgataccagg atttcaccgg
      841 cgccggcctt gccgaggatg cgcgccgcga tgtgtacggc gatcccgccg atgtcgttgt
      901 cgagcagttc gcactcgccg gtgtggatgc cggcgcgaat ctcgaggccc agtgtctcgg
      961 actcaacgcg caacgcctcg gcacagcgga tggcctgcgt cggcccctcg aaggtcatga
     1021 ggtggccgtc gccggtgctc ttgatcaccg tgccgccgaa acgttccgtc agttcggcgg
     1081 cgagctcacc aaagcgttgc agcaccgcac gccaccgctc gtcgcccgtc gccgccgcct
     1141 ggtgggttga ggcgactatg tcggtgaaca acaccgtgcg cagggcgcga tgcgactgcg
     1201 ccggtgcggc atggctgccg gtcaggaact cctcgatcac ggtaaagatc cggtcgggat
     1261 cggcgagcca ggcatggtcc ctgccctcga cttcgagcaa ccgcgcaccg gggatgtggt
     1321 cggccatgtc ccggccgaac tgcaccggaa cgagttcgtc gcgggcgtgg atgaccaggg
     1381 tgggcacggt gagcgtcggc aggatcggcc gcaagtcaat ccgaaacatc gcttcgagcc
     1441 ccgcgcgcac catccccgga ctggcgcaca tgcgctcaag catggccaac aggcggatcg
     1501 acatcgacgg cagcagaacc ttgctcgcct cgccgctgcc ccacgccgag acgacggcgc
     1561 ggccccaagc ctgccagcgg gcgagctgct gcaccgaggg cgtgtagacc tcacccagct
     1621 cgcgcaccag gcgcgcccgc agctcgtccg gatcgcggtc caagtcatcc cagctgtcgg
     1681 cgacggagta cgcggcggtg ccgttaagaa tcaacgcccg cgttcgctgc ggtcgtgttg
     1741 ccgcgaagaa gatggaggcc gggccccccg cgctaggccc gaaaagcgcc gctcgttcga
     1801 aaccgacggc gtccatgacg gcctcgatct cggccgcccg atcatccatc gtgcgaacat
     1861 gcgggaccgg gtccgacagc ccgaccccgg catagtcgaa cagaacgacg cgacagaacg
     1921 tggacagccg ctcgaacaag accacaaatt cgggcacggt ccagtacagc tcgacgtggc
     1981 tgacgtacga cccggaaaag acgagctcga caggcccgtc cccgaacacc tgataggcca
     2041 ggctcagatc accgcacttg gcgtacgacg tttccggcac gctctgagcg tacttcggcc
     2101 gggcgctagc cctaccgaat ccactgcatc gcgcccgggc gtacgacaac gccgccatga
     2161 tcgagccgct gcgcgacctt cccagccagg actatggcgt caagcacaag ctcgacccgc
     2221 cgtccgcccg ctgtccgtcg cccgcttcat ctatcggctc ttccgactcc acgacggcaa
     2281 gggcgccagg actctcttca ggtggcacgg cacctggatg ctctggcatc aatcgcactg
     2341 gtcggagctg aacacgccgc aactaggcag ccacgtctac cgcacgctgc acaaggccat
     2401 ctacgaggtt aaaaagcgcg atgacatcga gatcaagccg tggaatccgg acaagcacaa
     2461 ggtggccaac gttctggagg cgacggaagc gctcgcgcac ctagacgccg agattcaccc
     2521 gccagaatgg atcggcctac atagcgcggc ggaaaccgct gcagcgcaga tgatctcgtg
     2581 cgaaaacgct ttactcgacc ctgtcgaccc ggcagaccca tcggcacaca ccgacgctgt
     2641 tcaatgtcgt gtccgtgccg ttcgcctacg acgagaacgc gccggaacct gtcgcgtggc
     2701 ttgagttcct ggaatcggtg tgatcgagcg acaagaatcg gctgcgctgc taccgtcgcg
     2761 cggtgccaac ggtgtgatgg ataccaacag aagggagcct cacgatgacc gacctacgtg
     2821 accgcattat taaggccttc cagaaacggg tgggcaaccc gctgacgcga cgactgccca
     2881 cccagacgtt gctcgaaacg acgggacgca agtcaaggca gccacgccgc actccgctgg
     2941 gcggcagccg ggtcggcgac cagttctggt tcgtgtcgga gttcggcgag aagtcgcagt
     3001 acgtgcaaaa catcaaggcg aaccccaacg tccgagtgcg gttgggcggg cgatggcaca
     3061 acggcacggc gcatctcgtg cccgatgacg acccgcgcgc acggctgcac tcactgccgc
     3121 agctcaacag tctcgccgtc cgcgccgtgg gcacgaacct gctcacgatc cgggtcgacc
     3181 tgacggactg aggcattgac tcatacctcg ccgcgtcaaa gcgctggcac ttcagcgaag
     3241 gctagcgcct ccggcgagtt ccgctccggc gcgacgctcg ctggcgcgcc gcgtcatcga
     3301 tacgagcgac gaccatgacc gggccgactg gcagcgccag caaacaccgg caaaccggcg
     3361 tcatcgcata accatgggta ctcatttttt ggagcgacga cggccacccc tccggtttgc
     3421 gtggtcgatc tgtccgcgat gatctccaga attttcggaa gatttacggt cggcggcccg
     3481 aaatcacggg atcattcctg ccgaccggtg gccgcgagct aatcatgaaa tccattgtag
     3541 cacttggcgt ttcgacaagc gtctcgaatc gagatgcgcg attttgcaac ggaaatcgcg
     3601 aagcagcagt cgtgacggaa aatttccggt caccgaccgt acaaaccgtg accgtttcgc
     3661 gcgcatacgt cgatattctg ccggacgaaa cggcttggtg gggggtatgc cgtaatcacc
     3721 ccgcgccagc aaacatttat caacaagcat cactggcgac caagatcaag ttgcccaccg
     3781 ttgtgggttc cgcgccgccg ctgtggcgct catgcgcagc tgaagagccg ttgttctcac
     3841 cccgtcgaaa tctccatgac tgcacacact cccccacggc gggcgaaaga aggctatcga
     3901 catgtccgca aaaactgata tcgatcaact ggtgctgctc gccgatgatg cgctcaacag
     3961 ccgcgtcgag gcggccgcgg cccgcatcga aacccgcatc gaacgcgcga tggacggagg
     4021 ccgtgatctg acgcagcgtg aatacgaatt gacgcagcag gaccgcgacg aattggtcgc
     4081 actgcgtgac gccacagaga tccggtctgt gcgcggcgac caatccgacc gcatcgagca
     4141 ggagacccgc gaccgcgccc aactgatcgg tggcgtcatc gagacgcgcg gcaacttgtc
     4201 cccgctgttc ccgtccgcgg agaacctggc ccgtctggaa gaagcgcggt ggcagcagtc
     4261 gaccctgacg ctggtcaagg aacgcgcggc gatcaagacc accgacgcgg gcaccgcccg
     4321 cgagtacgcg ccgtcgggcc tgttggcacc gcgcggggga tgcctccgcc cgactcggcg
     4381 cggacaccca gcgggcataa cgtctcgggt tctgtacggg ttatgagtcg aactcgggag
     4441 ttcaaccgca cgcctctcgc cgagccgttg aggggtgatc tctgctggtg aggagcggac
     4501 ggattgaaac ttggacggcg acagtcccgt gcggttggtg cggtatctat gtgtctgaaa
     4561 cggactcggg tcgcggtcgt ctgccatgcc ggctggtatc tgcgccgagc gaacagagtc
     4621 tttagactac tcggcgacac aggcgttttg agtcatcgct catgcagcgg cgaaccactc
     4681 acttcgatcg cccgcgcctc acgggtgtgt tggctaccag agcgtgaaag gtccggcagt
     4741 tcgacgtatt cggagattcc gatatgccgg tgcagccgca cgagcggtgc gggtgcccac
     4801 cagttaaccc ggcccagcac acgcatgaac accggaacaa gcaggatccg aaccagcgtg
     4861 gcgtccacta agaccgccaa cgtcacgccg acaccgaaca tgcacattag cgagaccttc
     4921 gcggccgcca gcgacgagaa cgttaccgcc atcagcaggg ccgcggcggt caccacccgg
     4981 ccggtgcggg ccagtcccaa agccacgctt tctgtattgt cggcccgcgt ttttcccgat
     5041 gcctgccaat actcgccgat ccgcgagatc aagaacacct cgtagtccat cgacagcccg
     5101 aaggcaaggc agaacagcag cacaggaacg ctggcgatga gcgtgcccgt ggccgtcgtg
     5161 ccaaacccgt ccaggtggcc gtcctggaag atccacacga gtgcgccgaa cgccgccgtc
     5221 aacgacaaca tgttgagcgc caacgccttg agcggcagta tcacgctgcc ggtcagcaag
     5281 aacagaagca ggaaggtgat cgttgccata accgacagca caatcggcag ccgtgaggtg
     5341 accgcgtgcg agctgtcgcg gttgatctgt gcccagcccg tcatctgcac agttgcgttg
     5401 ccgggagttg caatggcgtg caaccggtcc agctgagtct ccgaggcctg ggagtacagc
     5461 ggcgcggtgg agttcacggt cagataggcg ctgccgtctt tccatcccgt gggcgccgag
     5521 ggcggcccgc ccaggacgcc gctggcgtac gtgccggcgg gtgatgacac agccgagacg
     5581 tcgggtaccc gtgacagctg cgcggcatag aagccgagtt cggccgctgt cactgccccc
     5641 gtgtcgggaa tgacaacgat cacgttggtc aacgagttga ccgagaagcc ggatcgcagc
     5701 tcgtctccga cctggcgcgc tgaggcggaa accggcagca ccctgtcgtc ggggtagccc
     5761 catttgacgc cgaggaacgg ggcgcccaac acgagcagca cggcgatgac cgccaccccg
     5821 atcggaatcg ccgggcccat aatggctttc gtccagccgt accagaacgt ctcgtccacg
     5881 ggccggtgca ccggctcggg ccgccccaac acgcctcgga cgacgcggcg cacgtcgagt
     5941 gcgtcgagcc gatcacccag cagcacgagc gccgccggcg tcaccaccac cgcggcagcg
     6001 gccgcgaacg cgaccacggc gattcccgca taggcgaaag acttcaggaa gtacatcggg
     6061 aagatcacca tcgcgatcat cgccagcgcg accgtggttg ccgagaacag caccgtccgg
     6121 ccagccgtgg tcatcgtgcg caccaacgcc tggtcccgtc ctgcgccgtc agccaattcg
     6181 tcacggaacc ggctgacgat tagcagggtg taatcgacgg ccaacgcgag ccccatggca
     6241 aggatcaggt tcaacgcgaa gaccgagact tcggtggcga acgtaatcgc acgcatcacc
     6301 gccatcgagc cgaggatcgc gaagatgcca accgccaacg gcagccccgc cgcgagcagg
     6361 ccgccgaaaa cccaaacgag cacaatgaaa ctcagcggaa acgcgatggc ctccatcacc
     6421 aagaggtctt tcttggtttg gtcgatgatc tgggcgtagg tgatcacctc gtcaccgggc
     6481 ttcactgtga cgccgtcgaa gcggggcagc gaaccgagca ggtccttggc gtgctgctgt
     6541 gcgccggtct caccgccggt gatgccggcc accaccagac cggtcttccc gtcctcgctg
     6601 atcagcgctc gcgcggccgg gggaggtact gtccatgcgg atttgacgtc ggcgacgtag
     6661 ggcaagctct tcagcagcgc gaccaactcg tc
//
LOCUS       DASRJA010000402         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_283135,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000402 DASRJA010000000
VERSION     DASRJA010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283135"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFC01_17630"
     CDS             complement(<1..543)
                     /locus_tag="VFC01_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HZN81486.1"
                     /translation="MLISGRRAGVVAASTAVIAAVVMTLASSCGSHGESPASPAGQQS
                     VITSTTKIASAGVLGNQRRPDESCAPDPAALDPGPPDREVRHAAGTTVVRADPQRIVV
                     LSGDQLDALCALGLQSRIVAAALPDGSTSQPSYLGTVVHDVPGAGSRSEPDLAAIKAA
                     TPDLILGSAALTPDAYPELTA"
     gene            713..2440
                     /gene="ctaD"
                     /locus_tag="VFC01_17635"
     CDS             713..2440
                     /gene="ctaD"
                     /locus_tag="VFC01_17635"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZN81487.1"
                     /translation="MVAEAPPIGELEARRPFPARLGPKGNLVYKLVTTTDHKLIGIMY
                     CVACFIFFFIGGLMALFMRTELALPGLQFLSNEQFNQLFTMHGTVMLLFYATPIVFGF
                     ANLVLPLQIGAPDVAFPRLNAFSFWLFLFGATIAIAGFITPGGAADFGWTAYSPLTDA
                     IHSPGAGGDLWIMGLAVGGLGTILGGVNMITTVVCMRAPGMTMFRMPIFTWNILVTSI
                     LVLLAFPLLTAALFGLAADRHLGAHIYDPANGGVLLWQHLFWFFGHPEVYIIALPFFG
                     IVTEIFPVFSRKPIFGYTTLIYATLSIAALSVAVWAHHMYATGAVLLPFFSFMTFLIA
                     VPTGIKFFNWIGTMWKGQLTFETPMLFSVGFLVTFLLGGLSGVLLASPPIDFHVTDSY
                     FVVAHFHYVLFGTIVFATYAGIYFWFPKMTGRLLDERLGKLHFWLTFIGFHTTFLVQH
                     WLGDEGMPRRYADYLPTDGFTTLNVVSTIGAFILGISTLPFTWNVFKSWRYGEPVMVD
                     DPWGYGNSLEWATSCPPPRHNFTELPRIRSERPAFELHYPHMVDRMRAEAHVGRHHEV
                     VAEPVDQSS"
     gene            2469..>2794
                     /locus_tag="VFC01_17640"
     CDS             2469..>2794
                     /locus_tag="VFC01_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZN81488.1"
                     /translation="MPKVSVLITVTGPDQPGVTSALFEVLSRHKVELLNVEQVVIRGR
                     LTLGVLVAAPPEVAGGAELRREVEDAIHGVGLEVTIERSDSLPVMREPSTHTIVVLGR
                     PITAES"
BASE COUNT          412 a          937 c          908 g          537 t
ORIGIN      
        1 cgcggtcagc tccggataag catcaggcgt cagcgccgcg gagcccagga tcaggtcggg
       61 cgtcgcggcc ttgatcgccg ccagatccgg ttcactgcgc gagcctgcgc cgggcacgtc
      121 gtgcacgacg gtgccgagat acgacggctg gctggtcgag ccgtcgggca aggcggccgc
      181 gacgatgcgc gactgcagcc ccagcgcaca gagcgcgtcc agctggtcac ccgacagcac
      241 cacgatgcgc tgcgggtcgg cacgcacgac ggtggtaccg gccgcgtggc gaacctcccg
      301 atccggcgga cccgggtcga gcgccgcggg atcgggtgcg cacgattcgt ccggcctgcg
      361 ctgattgccg agcacgccgg cgctggcgat cttggtggtg ctggtgatga ccgactgctg
      421 gcctgccggt gacgccgggc tttccccgtg cgatccgcag ctgctcgcga gcgtcatcac
      481 gacggcggca atcacggcgg tcgaggcggc aacaacgcct gcccgccgcc cgctgatcag
      541 cacgccgccg acggtaacat tcgggccgct cgccgctggt caggccgcca ggctgggacg
      601 tcgctgggta tttacgacag tttgtaggac ccatgccgac accgggcccg tcgagagata
      661 ggattcggga caagactgcg gggatgtccc gactggatgt ttggaggacc tgttggtagc
      721 cgaagcgccc ccaatcggag aactcgaggc tcgccgtcca ttcccggccc ggctcggtcc
      781 taagggcaac ctggtctaca agctggtgac caccaccgat cacaagctga tcgggatcat
      841 gtactgcgtc gcctgcttca tcttcttctt catcggcggg ctgatggcgc tgttcatgcg
      901 caccgagctc gcgctgcccg gcctgcagtt cctgtccaac gagcagttca accagctgtt
      961 caccatgcac ggcacagtga tgctgctttt ctacgcgacc ccgatcgtgt tcgggttcgc
     1021 caacctggtc ctgccgctgc agattggtgc gcccgacgtg gcgttcccgc ggctgaatgc
     1081 gttctcgttc tggctgttcc tgttcggcgc gacgatcgcg atcgccggct tcatcacccc
     1141 aggcggcgcc gccgacttcg gctggaccgc ctactcgccg ctgaccgacg cgatccactc
     1201 gcctggcgcc ggcggtgacc tgtggatcat gggcctggcc gtcggtggtc tcggcaccat
     1261 cctcggcggc gtcaacatga tcaccaccgt ggtctgcatg cgcgctcccg gcatgacaat
     1321 gttccggatg ccgatcttca cctggaacat cctggtgacg tcgattctgg tgctgctggc
     1381 cttcccgctg ctgaccgccg cgctgttcgg tctggccgcc gaccgccacc tcggtgcgca
     1441 catctacgac ccggccaacg gcggtgtgtt gttgtggcag cacctgttct ggttcttcgg
     1501 ccaccccgag gtgtacatca tcgcgctgcc gttcttcggc atcgtcaccg agatcttccc
     1561 ggtgttcagc cgcaagccga tcttcggcta caccacgctg atctacgcca cgctgtcgat
     1621 cgccgcgctg tcggtcgcgg tatgggcgca ccacatgtac gcgacgggcg cggtgctact
     1681 gccgttcttc tccttcatga ccttcctgat cgcggtgccg accggcatca agttcttcaa
     1741 ctggatcggc acgatgtgga agggccagtt gacgtttgag acaccgatgc tgttctcggt
     1801 gggcttcctg gtcaccttcc tgctgggcgg tctgtcgggt gtgctgctgg ccagcccgcc
     1861 gatcgacttc cacgtcacgg actcgtattt cgttgtggcg cacttccatt acgtgttgtt
     1921 cggcacgatc gtgttcgcga cctacgcggg catctacttc tggttcccga agatgacggg
     1981 ccggctgctc gacgaacggc tgggcaagct gcatttctgg ctgacgttca tcggcttcca
     2041 caccacgttc ctggtgcagc actggctggg cgacgagggc atgccgcgcc gctacgccga
     2101 ctacctgccc accgacgggt tcaccacgct gaacgtcgtg tcgacgatcg gggcgttcat
     2161 cctcggcatc tcgacgctgc cgttcacgtg gaacgtgttc aagagctggc gctacggcga
     2221 accggtcatg gtggacgatc cgtggggcta cggcaactca ctggagtggg cgaccagctg
     2281 cccgccgccg cggcacaact tcaccgagtt gccccgaatc cgttcggagc ggccggcatt
     2341 cgagctgcac tacccgcaca tggtggaccg catgcgcgcc gaggcgcacg tcggccgcca
     2401 ccacgaagtc gtcgcggagc cggtggacca atccagttaa cggtccatcg gggcgcgcgg
     2461 gggtgagcat gccgaaggtg tcggtgctga tcaccgtcac cggacccgat caaccgggtg
     2521 tgacgtcggc cctgttcgag gtgctgtcac ggcacaaggt cgaactcctc aacgtcgaac
     2581 aggtcgtcat ccgcggccgc ctgacattgg gtgtcctggt ggccgccccg cctgaagtgg
     2641 ccggtggtgc ggagctgcgc cgagaggtgg aggacgccat ccacggcgtc ggcctcgagg
     2701 tgacgatcga gcgcagcgat tcgttgccgg tgatgcgtga gccgtccacc catacgatcg
     2761 tcgtgctggg ccggccgatc acggcggagt cgtt
//
LOCUS       DASRJA010000403         2934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_419191,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000403 DASRJA010000000
VERSION     DASRJA010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419191"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFC01_17645"
     CDS             <1..392
                     /locus_tag="VFC01_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN81489.1"
                     /translation="AAEDAGDELARTDRGDEALDQLNAAFDTYLRHEALADARRVGRA
                     LRGLGVQRRIVTHPRAKTGWDSLTDSELTVVNLIAQGVTNRSVATQLHLSPHTVRAHL
                     RNAFAKLGVSSRVELSQLVRGAEHHAN"
     gene            647..2110
                     /locus_tag="VFC01_17650"
     CDS             647..2110
                     /locus_tag="VFC01_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN81490.1"
                     /translation="MSAHSPSSVAESASSTIDPDAQARRANDGEPSAGFPSDLTIDED
                     AVDGLLTAEEFVAEFDQNRDDGTLYAEYRRLAAEQAALRRVATLVARGVEPLEVFAAV
                     AEEMRRCLPADTAGLWRFESDREITLVAAAAAPEALTRWPVGTRTLVDGDTLATLVQR
                     SGRPARIDSYDNIAGPIAARVRAVGVRAAVAVPIIVDGRVWGLAALGSLQQRPMPADT
                     EVHICRFAELIATALVAGYRDEQNRQLLAEASRRSNLIDALLEGRAFDDWSLRDLAGC
                     LRLPSNGPFVVIAAHVPTVGDEALPEIESKLRSLDVFSAWRLLPDVQVGIAHVASDQK
                     LDMVVALMSRMTTVRVGVSAAFGDLRETPRALHVARVMVRGPTDSTSSVAVFDGSILA
                     TAAVSAPAAMIQTVGAALDGFGDLPDYDRGLLFETFRVWQDNDASVCAAAAVLTCHPN
                     TVRHRLRRIEKHSGRSLSRPRDVAELCLAFEVHRRLM"
     gene            2467..>2934
                     /locus_tag="VFC01_17655"
     CDS             2467..>2934
                     /locus_tag="VFC01_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine dehydratase"
                     /protein_id="HZN81491.1"
                     /translation="MSSDSLVAPTVSTGFDINAHFRAVMSDLGLSPEDTGGTITFVGK
                     DPIFPSVHRLGACIGIPIMAGAAGIADIWRQRSGVGQDLSLDLRKAIHGINPMYKFMP
                     TINGHPYQLPYFIGNPMGFDLYQTRDGRFFLPTGAYPRLMNDMCTFLECAPDKN"
BASE COUNT          526 a          940 c          933 g          535 t
ORIGIN      
        1 cggctgccga ggacgccggc gatgaactcg cacgcaccga ccggggtgat gaggcgctgg
       61 atcagctcaa tgccgcattc gacacctacc tgcgccacga agcgctcgcc gatgcgcgcc
      121 gggtcggtcg cgcgctgcgc ggactgggtg tgcaacgtcg tatcgttacc cacccgcggg
      181 caaagacggg ctgggacagt ctcaccgact ccgagctcac ggtggtcaac ctgatcgccc
      241 agggcgtcac caaccgctcc gtcgccacgc agctgcacct ttcccctcac accgtgagag
      301 cccatctgcg caatgcgttc gccaagctcg gggtcagctc ccgcgtcgag ctgagccaac
      361 tcgtgcgcgg cgccgaacac cacgctaatt agattctggg gacttagccc taggcgactt
      421 tggccttaga gataggccgc tggccaatgg tgttacccac gcccatgcgg cgactgtagt
      481 tgttgtcaac taatcacgtt ctcactcgaa agacggccaa ggcaaggcca gacaccgcgc
      541 tcgtgagcac acgaggaaaa gagcgaaaga tgctgagtgc aacgcttgga atgctggcga
      601 tggtgccggt ggtgttcggt gtcgccgggt gggcgatggt gtcccgatga gcgcacactc
      661 gcccagctcg gtcgccgaat cagcaagtag cactatcgat cccgacgcac aggcaagacg
      721 cgccaacgat ggcgaaccga gcgcagggtt tccatctgac ctgaccatcg atgaggatgc
      781 ggtggacgga ctcttaaccg ccgaggaatt tgtcgccgaa ttcgaccaga accgcgacga
      841 tggcacgttg tacgccgagt atcgtcggct cgccgccgaa caggccgcgt tgcgccgagt
      901 ggccacgctc gtcgcgcgcg gcgttgaacc tttggaggta tttgccgcgg tggccgagga
      961 gatgcgccga tgcttgcctg ccgacaccgc cggcttgtgg cgcttcgaat cggatcgcga
     1021 gattaccctc gtggccgccg ctgccgctcc tgaggcgctg acacgatggc cggtgggcac
     1081 ccgaaccctg gtggacggcg acaccctcgc cacgttggtg caacgcagcg gcaggcccgc
     1141 acggatcgac agctacgaca acatcgccgg gccgatcgcg gctcgcgtgc gtgcggtcgg
     1201 cgtgcgcgct gcggtggcgg tgccaatcat cgttgatgga cgcgtgtggg gcctggcagc
     1261 tctcggttcc ctgcaacaac gcccgatgcc tgccgacacc gaggtccaca tctgccgctt
     1321 cgccgaactg atcgccacgg cactggtggc cggttatcgc gacgagcaga acaggcaact
     1381 cctcgccgag gcgtcacggc ggtcgaatct gattgacgcg ctgctggagg gacgggcctt
     1441 cgacgattgg agcctgcggg atctcgccgg ctgtcttcgg ctgccgagca atggtccttt
     1501 tgtggtcatc gccgcccacg ttcccaccgt gggtgatgag gcgctccccg agatcgagtc
     1561 gaagctgcgt agcctcgacg tcttttcggc gtggcggctg ctgcccgatg tgcaggtcgg
     1621 gatcgcgcac gtcgcgtccg accaaaaact cgacatggtt gttgccctaa tgtctcggat
     1681 gaccactgtg cgggtggggg tcagtgcggc cttcggggat ctgcgcgaga cgccacgagc
     1741 cctgcatgtg gcacgggtga tggtgcgggg cccgaccgac tccacctcct cggtcgcggt
     1801 gttcgacggc tccatcctgg ccaccgctgc cgtgagcgcg ccggcggcga tgatccaaac
     1861 ggtcggcgct gcgttggatg gttttggcga ccttcccgac tacgaccggg ggctgctgtt
     1921 cgaaacgttt cgggtgtggc aggacaacga tgcgtcggtg tgcgctgcgg ctgcggtact
     1981 tacttgccac ccgaacaccg tgcgccaccg cttgcgccga atcgagaaac acagcggccg
     2041 atccctctcg cggcccaggg atgtcgccga actgtgtttg gctttcgagg tacatcgccg
     2101 tctgatgtag ccgaaaccca ttgacgctgt cgatgagtgg gaaggtcgtc acggtggaat
     2161 atcgcgtcgt ctgggcatgc gcatacgtcg tcatgatcga gcacttctgg tgactcgcga
     2221 ggtgggcgtg tcacagtttg cgatgtctga cgcgacatcc atgctgcacc aacgctataa
     2281 ccgacgctat ccgtagcttt ccagtacagc ccaggccgca cccgccaacg gcaatcgcgc
     2341 gccggtgttc cctgctacac ccaattggat tcagcgctgt cattggggcc aatgacgtgg
     2401 gttcccttac gggcaatagt cagggcatga cagccggggc ggcttcgaaa gttgaaagga
     2461 accaacatga gcagcgatag tttggttgca ccaacagtgt ctacgggctt cgacatcaac
     2521 gcacatttcc gggctgtcat gagcgacctc ggactgtcgc ccgaagacac cggcggcaca
     2581 ataactttcg ttggcaagga tccgatcttc ccgagcgtgc atcggcttgg ggcgtgcatc
     2641 ggcatcccga tcatggccgg cgccgcgggc atcgccgaca tctggcgcca acgcagcggc
     2701 gtaggccagg acctgagcct ggacctgcgc aaggccatcc acggcatcaa ccccatgtac
     2761 aaattcatgc ccacaatcaa cggccacccc taccaactgc cgtacttcat cggcaacccg
     2821 atggggttcg acctgtacca gaccagggac ggccggttct tcctgccgac gggggcctac
     2881 ccgcgcctga tgaacgacat gtgcaccttc ctggagtgcg cgccggacaa gaac
//
LOCUS       DASRJA010000404         7834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2824610,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000404 DASRJA010000000
VERSION     DASRJA010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2824610"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFC01_17660"
     CDS             <1..507
                     /locus_tag="VFC01_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81492.1"
                     /translation="IAMRLYATVTIPNLPAFIKDPNHKGLWRAAGSIPVLGGDVIDMG
                     YSDFGLFRRAIRTGKGVREMVYDTTIAVGGERYTMHGRKYVEPSPPWRLWPATTTLYV
                     QLLPPSGGEPVAAGILRLTLWAFLRQLFSMQVTGDFSVFDRWLHRLSFYRFFVSSLIN
                     TYIKGRRW"
     gene            504..2111
                     /locus_tag="VFC01_17665"
     CDS             504..2111
                     /locus_tag="VFC01_17665"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZN81493.1"
                     /translation="MSSEERADVVIVGSGFGGAVAACRLAADDRRVVVLERGRDWSET
                     KTPQRARDYLYSSRMPHWLNGWLDIRFLDQMVVATGAGVGGGSLIYANVCVDAPPVVF
                     RTGWPSQITAEEMQPYYGKVTKVLKPQEIPASQLNPRMAMLSSAAGALDIGARYRPLP
                     LAVAFDETVADTRKEPWRDPGVYAPGTCVHCGNCVIGCEHGAKHTLDQNYLSEAKKHA
                     AEIWPLSMVTHIEEGPDGAWRVHYLDVGAGVGRRKRQIVGKLVILAAGSIGSTEILLR
                     SRDGFRTLKNLPRALGRGWSPNGDFLTLARYRNGRLLEPTKGPTIGGVIDFLDGVDLP
                     DDNMVGYDGRIYIEDGGLPNIGAHLLKSWKDARGLKGMLYRKASSLTDFSDMVPWFGQ
                     SIDAADGEFSLQNPLKFWRARTRLDWNPQRSASALDAFKDVHVAMTRATGGKPIPLVT
                     WSRLRTLVTPHPLGGCNMAAAAGAGVTNHMGEVFGYDNLFVMDGSVIPRAIGRNPSKT
                     IAAVAERSCEKLLEELPRRLNGAMTET"
     gene            complement(2108..3070)
                     /locus_tag="VFC01_17670"
     CDS             complement(2108..3070)
                     /locus_tag="VFC01_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN81494.1"
                     /translation="MSLRIGVLGASRIAELAIAGPAHELGHRLVAVAARDPQRAQSFA
                     EKHGVERVLATYDDVVGDPDVDVIYNPLANALHAPWNLAAVAAGKPVLTEKPFARNRA
                     EAQQVAAAADAAGVTVMECFHYVFHPVTRRALELVSDGTLGELVHVEVRMAMPAPEDN
                     DPRWSLGLAGGALMDLGCYGLHVMRQLGHPSIVHAHAEERSPGVDAWCDVDLAFPGGA
                     TGLSANSMVADDYSFTLRLVGTRGDVLVHNFIKPHDDDRLTINTSHGTTVERLGMRPS
                     YTYQLEAFAAHVQHGAPLPLDTADAVENMAYVDAAYRAAGLTPR"
     gene            complement(3067..3938)
                     /locus_tag="VFC01_17675"
                     /pseudo
     CDS             complement(3067..3938)
                     /locus_tag="VFC01_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241204.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(3935..4942)
                     /locus_tag="VFC01_17680"
     CDS             complement(3935..4942)
                     /locus_tag="VFC01_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN81495.1"
                     /translation="MTTLGVIGLGRIGAFHTETLSNLDGVDGLVVADERPDLVAAVAA
                     KHGAKPADSVEELLASGVDGVVVSAATPAHAELTLAAVHRGLPTFCEKPIAATAAESA
                     HVAEVIARSGVPVQVGYQRRFDAAFAAAKRAVDDGSLGLLHTVRSTTMDPAPPPIEYI
                     ASSGGIFRDCAVHDFDVLRWITGQQAVEVYATGSVQGDPRFTEYGDVDTAAVVVTFDH
                     GTLGVVSAARYNGHGYDCRLEVHGFHDTVVAGWDQGAPVRNVDPDNDFPAGPAHHFFM
                     DRFTDAFRAELSAFVDVVNGTAAPACTVADAVEVAWLAEAATESLRRGVPVRIEDVRS
                     S"
     gene            complement(4939..6102)
                     /locus_tag="VFC01_17685"
     CDS             complement(4939..6102)
                     /locus_tag="VFC01_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HZN81496.1"
                     /translation="MTAAVHATPKPWIDEDDLHLDDFRAQVLRSTDPADYPLATDVRS
                     NVLVYSTATVTGADRRALQSELIRALADGPGVVAFEGAFDVDVVDRASEAFFAIIDAQ
                     RAAGAAAGDHFGKPGANDRIWNAAQKLALHDPQVFADYYANDALAVVCQAWLGPRYQV
                     TSQVNAVNPGGKQQVPHRDYHLGFVADEDLASYPAHLHRTSPVLTLQGAVAHCDMPVE
                     SGPTMLLPFSQRFEGGYIAFNRPEFVDFFAEHHVQVPLAKGDAVFFNPALYHGAGSNV
                     SADIRRIANLLQISSPFGRAMEALDRTAMVRAVYPALRAMKAAGRPQRELVNAVVATA
                     EGYAFPTNLDSDQPIGGLAPHSQVDTVLAALADDLTAEELGVALSDQDDRRKP"
     gene            6178..7025
                     /locus_tag="VFC01_17690"
                     /pseudo
     CDS             6178..7025
                     /locus_tag="VFC01_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
     gene            7312..>7834
                     /locus_tag="VFC01_17695"
     CDS             7312..>7834
                     /locus_tag="VFC01_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZN81497.1"
                     /translation="MKGRRVGANGNGASPGTRQKLEKVVIRFAGDSGDGMQLTGDRFT
                     SEAALFGNDLATQPNYPAEIRAPQGTLPGVSSFQIQIADYDILTAGDRPDVLVAMNPA
                     ALKANVSDLPRGGLIIANSDEFTKRNLAKVGYEANPLETDELSDYVVQPVAMTSLTLG
                     AVESIGASKKDGQR"
BASE COUNT         1359 a         2562 c         2605 g         1308 t
ORIGIN      
        1 atcgcgatgc ggctatatgc caccgtcacc atcccgaacc tgcctgcgtt catcaaagat
       61 cccaaccaca aaggactatg gcgtgccgct gggtcgatcc ccgtgctggg cggcgacgtt
      121 atcgacatgg gttacagcga tttcgggctc ttccgccgcg cgatccggac cgggaagggc
      181 gtgcgcgaga tggtgtacga caccacaatc gccgtgggcg gtgagcgata cacgatgcat
      241 ggccgcaagt acgtcgaacc gtcaccaccg tggcggctgt ggccggcgac cacaacgctt
      301 tatgtgcaac tcttgccgcc cagcggcggc gaacctgttg cggcgggaat tcttcggttg
      361 acgttgtggg cgtttctccg gcagctcttc tcaatgcagg tcaccggcga cttctctgtg
      421 ttcgacaggt ggctgcacag gttgtcgttc taccggttct tcgtgtcgtc gttgatcaac
      481 acctacatca agggtcgacg gtggtgagca gcgaggagcg cgccgacgtc gtcatcgtcg
      541 gcagcgggtt cgggggtgcg gtcgcagcgt gccgcctggc cgccgatgac cgacgggtgg
      601 tggtgctcga acgcgggcgc gactggtcgg agacgaagac gccccagcgg gcccgcgact
      661 acctctactc cagcaggatg ccgcactggc tcaacggctg gctggatatt cggtttctcg
      721 atcagatggt ggtggccacc ggtgccggag tgggtggcgg ctcgctcatc tacgccaatg
      781 tgtgcgtgga cgcaccaccc gtcgtgttcc gtaccggctg gccatcgcag atcaccgcgg
      841 aggagatgca gccttactac ggcaaagtga cgaaagttct caagccgcaa gagattccgg
      901 caagtcagct caacccgcgg atggccatgc tgagcagtgc ggcgggggcg ctggacatcg
      961 gcgcgcggta tcgccccctt ccgctggctg tggcgttcga cgaaaccgtg gctgacacac
     1021 gcaaagagcc ctggcgcgat cccggcgtgt atgcgccggg cacgtgcgtg cactgcggga
     1081 actgcgtgat cggctgcgaa cacggtgcca agcacacgct cgaccagaac tacctcagcg
     1141 aggcgaagaa gcacgccgcg gagatttggc cgctgtcgat ggttacccac atcgaggagg
     1201 gaccggatgg cgcatggcgc gtgcattacc tcgacgtcgg ggcgggcgtg ggacgtcgca
     1261 agcgccagat cgtcggaaag ctggtcattc tcgcggcggg gtccatcgga tccaccgaga
     1321 ttctgttgcg ctcgcgcgac ggattccgga cgctgaagaa cctgcctcgc gcgctcggaa
     1381 ggggctggag tcccaacggc gatttcctca ccctggctcg ttaccgcaac gggcgattgc
     1441 tggagcccac caaggggccg accatcggcg gggtgatcga cttccttgac ggcgtcgatc
     1501 ttcccgacga caacatggtc ggatacgacg gccgcatcta catcgaagac ggcggtctgc
     1561 cgaacatcgg tgctcatctg ctgaagtcct ggaaggacgc tcgcgggctg aagggaatgc
     1621 tataccggaa agccagttcc ctcaccgact tcagcgacat ggtgccatgg ttcggccagt
     1681 cgatcgacgc cgccgacggc gagttctcgt tgcagaaccc gctcaagttc tggcgtgcca
     1741 gaacgcggct ggactggaat ccgcaacgtt cagccagcgc gttggacgcc ttcaaggacg
     1801 tccatgtagc gatgacgcgt gcgacgggcg gcaagccgat tccgttggtc acatggagtc
     1861 gcttgcgcac gctggtcaca ccccatccgc tcggcggctg caacatggcg gccgcggcgg
     1921 gggctggggt caccaaccac atgggcgagg tgttcggtta cgacaacctg ttcgtgatgg
     1981 acggatccgt aatcccaagg gcgatcgggc gaaatccgtc caagacaatc gcggcggtcg
     2041 ccgaacgcag ttgcgagaag ctgctcgagg agctgccccg ccggctcaat ggcgcgatga
     2101 ccgaaaccta gcgcggagtc agacctgccg cgcggtaggc cgcgtcgacg taagccatgt
     2161 tctccaccgc gtcggcggtg tcgagaggta gcggcgcgcc gtgctgaaca tgggcggcga
     2221 atgcctcgag ctgataggtg tacgacgggc gcatgcccag ccgctcgacc gtggtgccgt
     2281 gcgatgtgtt gatcgtcagt cggtcgtcgt cgtgcggctt gatgaagttg tgcaccaaca
     2341 catctccgcg ggtaccgacc agtcggagcg tgaacgagta gtcatcggcc accatcgagt
     2401 tcgcgctcag ccctgtcgcc ccgccaggga aagcgaggtc gacgtcgcac cacgcgtcga
     2461 cgcccgggct gcgctcttcg gcgtgggcgt ggacgatcga cggatgtccc agctgccgca
     2521 tgacgtgcag gccgtagcag cccagatcca tcagcgcgcc gccggccaac ccgagcgacc
     2581 accgcgggtc gttatcctcc ggcgcaggca tggccatccg cacctcgaca tggacgagtt
     2641 caccgagtgt gccgtcgctg accagttcca gtgcgcggcg cgtaaccgga tgaaacacgt
     2701 agtggaagca ctccatcacg gtgaccccgg cggcgtcggc cgctgcggcg acctgctgag
     2761 cttcggcccg gttgcgggcg aacggtttct cggtgagcac cggcttacct gccgcgaccg
     2821 ccgcgagatt ccacggcgcg tgcagcgcat tggccagcgg gttgtatatg acgtcgacgt
     2881 cgggatcacc gaccacgtcg tcgtaggtcg ccaaaacgcg ttccacgccg tgcttttcgg
     2941 cgaacgattg cgcccgctgc gggtcgcgcg ccgcgaccgc gaccagccgg tgtcccagct
     3001 cgtgcgccgg cccggcgatg gccagttcgg cgatccgcga cgcgccgagc accccgatcc
     3061 gcaaactcat gccgggacgg acccgcacac gtcgttcatg tacgcgacgc tggcgcgcac
     3121 gtcgcgcact gggccctcat cggttggctc gtcctcgaga atggtgtcct gctccatgac
     3181 gaaccagccg tcaaagccgt tgccgcgcaa cgccgtgacg atgcctgcaa tgtcgacatc
     3241 gcctccgccg agcggcgtat acatgccctc gcgtaccgcc tcggtgtagg tcagctcacc
     3301 ggcttgcacg ccggcagcga gggcggcatc gacgtccttg agatgggcgt gggcgatacg
     3361 gcccggcacc tcgcgggcca gttgcagcgg atccgttccg ccgatcaaca ggtggccggt
     3421 gtccaggcac agcttgatct gcgagccggc caggacgcgg tcgacgtcgt tgcgggtctc
     3481 caccatcgtg ccgacgtgcg ggtgcagcac cgcgagcacg cccctcttcg ctgctgcggc
     3541 tgccagccga tcgagattgt cgagcagggt agcccattga tcgtcgtcga gcgtcgggcg
     3601 tgagtcatat ccgccggcgc ctgtcgccgc ggccagcacg agaacgttgg cgccgcatgc
     3661 gacgagagcg tcgaccgggc ctgcgatgtc gggcagcgga tcgtgccgag catcgtgcag
     3721 cagaaccggc gcgaaggtgc ccatacagct cagcccatag gaatccagca gagcggtcag
     3781 ctcgtcggga tcggacggca ggaagccctc gggtccgagt tcggttgcgg tcaggccgac
     3841 gtcacgcatc tcggcgagta cgcgtggtgg gctgagctga tagcccaccc gggcacctcg
     3901 catacacccc aggagatcgg cgcgccagcg attttcatga tgacctcaca tcctcgattc
     3961 gtaccgggac accccgtcgc aatgactctg tcgccgcttc cgccagccac gccacctcca
     4021 ccgcatcagc gacggtgcat gcgggtgcgg cggttccgtt gaccacgtcg acaaatgcgc
     4081 tcagttccgc gcggaacgcg tcggtgaagc ggtccatgaa gaagtgatgc gccggacctg
     4141 caggaaaatc gttgtccggg tccacattcc gcaccggcgc gccttggtcc cagcctgcga
     4201 cgaccgtgtc gtggaagccg tgcacctcca gtcggcagtc gtaaccgtgg ccgttgtagc
     4261 gcgccgccga caccaccccg agggtgccgt gatcaaacgt caccacgacc gcggcggtgt
     4321 cgacgtcgcc gtattcagtg aaacgggggt cgccctgcac gctgccggtc gcatagacct
     4381 cgacggcctg ttgtccggtg atccagcgca gcacatcgaa atcgtggaca gcgcagtcgc
     4441 ggaagatgcc gccggaactt gcgatgtact ctatcggcgg gggagcggga tccatggtgg
     4501 tactgcggac ggtgtgcagc aggcccagcg agccgtcgtc gaccgcccgc ttggcggcgg
     4561 cgaacgcggc gtcgaaccgt ctctgatagc cgacctgtac cggcacgccg gagcgggcga
     4621 tcacctcggc gacgtgtgcg ctctccgcgg cggtcgcggc gatgggcttc tcgcagaacg
     4681 ttggcaaccc gcgatggacg gcggccaggg tcaactcggc gtgcgcgggc gtggcggccg
     4741 acaccaccac accgtcgacg cccgaagcca gcagctcctc caccgaatcc gctggttttg
     4801 caccgtgttt ggccgcgact gcggcaacta ggtcaggccg ctcgtcggcg acgaccaacc
     4861 cgtcgacacc gtcgagattg gacagcgtct cggtgtggaa ggcgccgatg cggcccaacc
     4921 cgattacgcc gagggtggtc atggctttct ccgatcgtct tggtcgctca gtgcgacgcc
     4981 gagttcttct gcggtgaggt cgtcggccaa cgcggccagc acggtgtcga cctgactgtg
     5041 cggggccagg ccgccgatcg gctggtcact gtcgaggttg gtgggaaacg cgtaaccttc
     5101 ggcggtcgcc accaccgcgt tgaccagttc gcgctgcggg cgccccgccg ccttcatcgc
     5161 gcgcagcgcc ggatagaccg cccgcaccat cgcggtgcgg tccagcgcct ccatcgcgcg
     5221 accgaacggc gacgagatct gcaagagatt ggcgattcgg cggatatcgg ccgaaacatt
     5281 cgatcctgca ccgtgataca gcgccgggtt gaagaacacc gcgtcgccct tggccagcgg
     5341 cacctgcaca tgatgctcgg cgaagaagtc gacgaactcc ggccggttga acgcgatgta
     5401 gccgccctcg aatcgctgcg agaacggcag cagcatggtc gggccgctct cgacgggcat
     5461 atcgcagtgc gccaccgcgc cctgcaacgt caacaccggc gacgtgcggt gcagatgcgc
     5521 gggataagag gccagatcct cgtcagccac aaagccgagg tgatagtcgc gatgcggaac
     5581 ctgttgcttc ccaccgggat tgacggcatt gacctgtgag gtgacctggt accgcgggcc
     5641 cagccacgct tggcagacga cggcgagcgc gtcgttcgcg taatagtcgg cgaacacctg
     5701 cgggtcgtgc agggcgagct tctgcgcggc gttccagatg cggtcattgg cgcccggctt
     5761 gccgaagtgg tcgccggccg ccgcaccagc ggcgcgctgt gcatcgatga tcgcgaagaa
     5821 cgcctcgctg gcgcggtcca ccacgtcgac atcgaacgca ccctcgaagg ccaccacgcc
     5881 cggtccgtcg gccagtgccc gaatgagttc ggactgcagc gccctgcggt cggcgccagt
     5941 gacagtcgcc gtcgagtaca ccagcacgtt gcttcgcaca tcggtcgcca gcggatagtc
     6001 ggccggatcg gtgctgcgca gtacctgcgc gcggaagtcg tcgaggtgta ggtcgtcctc
     6061 gtcgatccac ggcttgggtg tggcgtgaac ggctgcggtc atgccagtta gtcttcctgt
     6121 gacagcgacc gcattcaacg ccagaaaccc atcaaaaacc cctcagaatg gaacccggtg
     6181 ccgcatggct acaaggtccg cgagatcgct cagcagtccg ggctgagcga ggccactgtc
     6241 gaccgcgtgc tcaatgagcg gccgggggtg cgggagaaca ccagggcaga ggtcgtgcag
     6301 gcgatcgccg acctggacaa gcagcgcgcc cagctgcgcc tggtcggccg ccgctacctc
     6361 atcgatgtgg tcatgcagac gccgcagcgg ttttccgacg ccttccgcgc cgcggtggag
     6421 gccgaattgc ccgcgttcgc gcctgcgatg gtgcgggcgc gctttcatct gtgggaatcg
     6481 ggctcgacgg aacagatggt cgacgcgctc gcccgtctgc gcggcagcca cggcgtggtg
     6541 ctcaaggcgc aagacgaacc cgaagtggcc gaggccgtcg accgcctcgt cgacggcggt
     6601 gtgcccgtgg tgacctacgc gaccgacgta ccggccagtt ggcgctgcgc ggctccggcc
     6661 gggcgctggt ggaagtcagc gatagcgatg gcatcgacgc gaccaacgag cgactcgtgc
     6721 tcgaggcgct cgagcggcac cccaacatcg aggctgtcta ctccgtgggc ggcggtaatg
     6781 ccgccacggt cgcggcgttc gaacggctcg gccggccgtg ccgcgtcttt atcgcccatg
     6841 atctcgacgc cgacaaccgg cggttgctgc gggagggccg catttccgtc gtgctgcaca
     6901 acgacctgcg cgccgacgcg cggctcgcaa tgcggctcat cctgcaggag cgcggtgctt
     6961 tgccggtcga gccgtcgcga cccgtcccga tccaggtgat cacgccgtac aacgtgccga
     7021 gctagtgcta gcgggtggct accgatcggt aaccattttt aagagaatga cattctccga
     7081 gaagtgacgg aaaacacaat tgtgtctcag gccgtccgtc ggcacatggg ttgaacagtg
     7141 cgttcgatcc gaagtcaccc atgcgtcgcc tgcgcgtcgg gtaaagggcc gcctaagaga
     7201 agtgcaataa tcggtactgg cagtgacatc aaaactcatg cggatgtcca ccgacggcgc
     7261 gtcggcctga ccgcgactcg gaggacattc gccggacgat ctccgaaaag catgaaaggc
     7321 aggcgagtgg gggcgaacgg gaacggcgcc agtccaggga cgcggcagaa gctcgaaaag
     7381 gtcgtcattc gttttgccgg tgactctggc gacggcatgc agctcaccgg tgaccgcttc
     7441 acctccgaag ccgcactgtt cggcaatgac cttgcgacgc aaccgaatta ccccgcagag
     7501 atccgcgcgc cgcagggcac cctacccggc gtctcctcgt tccagatcca gattgcggac
     7561 tacgacatcc tgaccgcggg cgaccgcccc gacgtgctcg tcgcgatgaa ccccgccgcg
     7621 ctcaaggcca acgtctccga tttgccgcgt ggcggcctga tcatcgccaa ctccgacgaa
     7681 ttcaccaagc gcaacctcgc gaaggtcggc tacgaggcca atccgctgga aaccgacgag
     7741 ctttccgact acgtcgtcca gcccgtcgcg atgacctcac tgacgctcgg tgcggtggag
     7801 tcgatcggcg cgtccaagaa ggatggccag cgcg
//
LOCUS       DASRJA010000405         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1193053,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000405 DASRJA010000000
VERSION     DASRJA010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193053"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFC01_17700"
     CDS             complement(<1..431)
                     /locus_tag="VFC01_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN81498.1"
                     /translation="MSDGLFDVPGEASASAGGPVGASAPLAVKMRPATLDEVLGQDHL
                     LKPNSPLRRLVEGSGAASVILYGPPGTGKTTLASLISQATGRRFEALSALSAGVKEVR
                     AVIDIARRAAVHGEQTVLFIDEVHRFSKTQQDALLSAVENR"
     gene            472..867
                     /locus_tag="VFC01_17705"
     CDS             472..867
                     /locus_tag="VFC01_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN81499.1"
                     /translation="MTTKPVNLTEALESFDDIYSPRIVGQVNDYDVRIAHAHGEHVWH
                     VHEDTDEFFLVLDGEFHVAVRNDDGGQSTVVLRKGDTYVVPKGIEHKPSSPGASILMF
                     EPRGTSSTGDRHEGEIPDHIDSTTGHALG"
     gene            complement(864..1694)
                     /locus_tag="VFC01_17710"
     CDS             complement(864..1694)
                     /locus_tag="VFC01_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 domain-containing protein"
                     /protein_id="HZN81500.1"
                     /translation="MADRYGTDILANNPHRRRRSTEQPVELGMVVEDAQTGYVGAVVR
                     IEYGRMELEDRRGHRKPFPVGPGYLIDGKPVILTAPKRSAPTAPTRTASGSVAVQTTA
                     KVALASRIYVEGRHDAELVEQVWGDDLRVEGVVVEYLGGVDDLVGIVEEFRPGPGRRL
                     GVLVDHLVAGSKEGRIADAVRRGPGGAHTLVVGHPFIDIWQAVKPARLGISAWPVIPK
                     GVDWKHGVCKALGWPHSQQADIANAWQRIRGRVRDWNDLEPALIGRVEELIDFVTEGA
                     "
     gene            1718..2290
                     /locus_tag="VFC01_17715"
     CDS             1718..2290
                     /locus_tag="VFC01_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81501.1"
                     /translation="MLLNRDTSEGIANGDITLVLRRWDAPRAKAGGTQRTVAGTIRVD
                     DVAEYPGGYRVTAAQARAAGYPDAKTAQKELDRRPAKHTYVISVSYLAPDERPELAAD
                     DRLSAADVDAITARLARWDAAAETPWTRTYLEMIASNEAVRAPDLAARVGLDVPRFKR
                     RVRQLKGLGLTISLDVGYRLSPRGKAFLKR"
     gene            complement(2287..3066)
                     /locus_tag="VFC01_17720"
     CDS             complement(2287..3066)
                     /locus_tag="VFC01_17720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN81502.1"
                     /translation="MLLKDKVAVIYGAGGGIGGAVARAFAHEGARVFLTGRSLETVKA
                     AAAEIASAEAAEVDALDEQAVDSHLQSVIDSAGRVDISFNAIGIPNAEILGVPLTDLR
                     AEHFFEPIMSYTTSYFLTARLAARRMLPNRSGVIMTVTALHSRTGLPLVGGYGPAMAA
                     KEALTRDFSAELAPHGIRVVGLRPQGMPETRTIRDAFEPRAKASGMTWEQWQELLASR
                     THSRRLMALEEMANVAAFMASDRASGITGTTVNLTMGSLDD"
     gene            3148..>3534
                     /locus_tag="VFC01_17725"
     CDS             3148..>3534
                     /locus_tag="VFC01_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN81503.1"
                     /translation="MVTTDLVSRARAGDSDAFRKLTEPHRRELQVHCYRMLGSLQDAE
                     DALQETLLSAWQGLDGFEGRASLRTWLYRIATNRCLNARRSASRRSAKEWDMPAVEPP
                     EPTGFGEIPWLEPYPDALLDKATDVGP"
BASE COUNT          624 a         1200 c         1141 g          569 t
ORIGIN      
        1 atccggttct ctaccgccga cagcaacgca tcctgctggg tcttggagaa ccggtgcacc
       61 tcgtcgatga acaaaacggt ctgctcaccg tgcactgcgg cacgacgggc gatgtcgatg
      121 accgcgcgga cctccttaac gccggccgac agcgccgaca gcgcctcaaa gcgacggccg
      181 gtggcctggg agatcagcga cgccagggtc gtcttgccgg tgcccggcgg gccgtagagg
      241 atgaccgacg ccgcgcccga gccctcgacc agccggcgca gcggcgagtt gggcttgagc
      301 aggtgatcct gcccgaggac ctcgtcgagc gtggccggcc gcatcttcac cgccagcggg
      361 gccgacgcgc caacgggtcc gcccgcgctc gcactcgctt cgccggggac gtcgaacaga
      421 ccgtcggaca ccgttcagga ataccacggg aagcggacat acgatcggat cgtgaccacc
      481 aagcccgtca atctgaccga ggcgctcgaa tcgtttgacg acatctacag cccgcgaatc
      541 gtcggccagg tgaacgacta cgacgtccgc attgcgcacg ctcatggtga gcatgtctgg
      601 cacgtccacg aagacaccga cgaattcttc ctcgtcctcg acggcgagtt ccatgtcgcc
      661 gtgcggaacg acgacggcgg ccaatcaacg gtcgtgctcc gcaaaggcga tacctacgtc
      721 gtgccgaagg gcatcgagca caagccgtcg tcgcccggtg cctcgatcct gatgttcgag
      781 ccccgcggta cctcgtcgac cggagatcgt cacgagggcg aaatccccga ccacatcgac
      841 agcacgactg ggcacgcgct cggctaagcg ccttcggtca cgaagtcgat cagttcctcg
      901 acgcggccga tcagcgcggg ctcaagatcg ttccagtcac ggacgcggcc ccggatgcgc
      961 tgccacgcat tggcgatgtc ggcctgctgg ctgtgcggcc agcccagcgc tttgcacacg
     1021 ccgtgcttcc aatccacgcc tttcggtatg acgggccacg ccgagatgcc cagccgcgcc
     1081 ggcttgaccg cctgccagat gtcgatgaac ggatggccga cgaccaatgt gtgggcaccg
     1141 ccgggcccgc ggcgaacagc gtcggcgata cggccttcct tcgagccggc caccaggtgg
     1201 tcgacgagca caccgagccg tcggcctggg ccgggccgga attcctcgac gatgccgacg
     1261 agatcgtcga cgccgccgag gtattcgacg accacaccct cgacgcggag gtcgtcgccc
     1321 cacacctgct cgaccagttc ggcgtcgtgt cggccctcga catagatccg gctggccagc
     1381 gcgaccttgg ccgtcgtctg gacggccacc gacccggatg cggtgcgcgt cggtgctgtg
     1441 ggcgccgaac gctttggcgc ggtaaggatc acgggcttgc cgtcgatcaa atagccgggt
     1501 ccgaccggga acggtttcct gtgcccgcgc cggtcctcga gctccatccg gccgtactca
     1561 atgcgcacca ccgcgcccac atagccggtc tgggcgtcct cgacgaccat gccgagttcc
     1621 actggctgct cggtggagcg gcgcctgcga tgcgggttgt ttgcgaggat atcggtgcca
     1681 tagcgatcag ccaccgagcg atcgtagggt caaagacgtg ctgctcaacc gcgacacgtc
     1741 cgaaggcatt gcaaacggtg acatcacact cgtgctgcgt cgctgggacg cgccgagggc
     1801 caaggccggc ggcactcagc ggacagttgc gggcacgatt cgcgtcgacg atgtcgccga
     1861 gtatccgggc ggctatcgcg tgaccgcagc gcaggcgcgc gctgcaggct atccagacgc
     1921 caagaccgca cagaaggaac tcgaccgccg cccggccaag cacacgtatg tgatcagcgt
     1981 gtcgtatctt gcgcccgatg agcggcccga acttgcggcc gacgatcggt tgtccgcggc
     2041 cgacgtcgac gcgatcaccg cccggctggc ccggtgggac gctgctgccg aaactccttg
     2101 gacgcgaacg tatctggaga tgatcgcctc gaacgaggcg gtccgggcac cggatctggc
     2161 ggcccgcgtc ggactggacg tgccgcgctt caaacgtcgt gtgcgtcagc tcaaggggct
     2221 gggtctgacc atcagcctcg acgtgggtta tcggctgtcg ccgcgcggca aggcattcct
     2281 gaagcgttag tcgtcgaggc tgcccatggt caggttgacg gtcgttcccg ttatcccgct
     2341 cgccctgtcg gacgccatga acgccgccac gttcgccatc tcctcgagcg ccatcagccg
     2401 tcgcgaatgg gtcctgctcg ccagaagttc ttgccactgc tcccaagtca tgcccgacgc
     2461 cttggcgcga ggctcgaacg catccctgat cgtgcgggtc tccggcatgc cctgcggccg
     2521 cagcccaacc acgcgaatgc catgcggcgc aagctcggcg gagaagtctc gggtgagcgc
     2581 ctccttggcg gccatcgcgg gaccgtaacc acccaccaac ggaagcccgg tgcgggagtg
     2641 cagcgcggtc acggtcatga tcaccccgga cctattcgga agcatgcgcc gcgcggcaag
     2701 gcgtgcggtc agaaagtacg acgtcgtata gctcatgatc ggctcgaaga aatgttcggc
     2761 ccgcaggtcg gtcaggggca cacccaggat ctcggcgttt gggatgccga tggcgttgaa
     2821 ggagatatcg acgcggccag ccgaatcgat cactgactgg agatgggagt cgacggcctg
     2881 ctcgtccagc gcgtcaacct ccgccgcttc cgcagaagcg atttccgcgg ccgcagcctt
     2941 cacggtttcg aggctgcgcc cggtgagaaa gaccctggcg ccctcatgcg cgaaggcgcg
     3001 tgcgacggca ccaccgatcc cgccgccggc tccatagatc accgcaacct tgtccttgag
     3061 caacatgtct gggtagacac cgcgccgccg gcgaagtggg cgtccaccaa ccgatcactt
     3121 tgacgcgaga tcggtgtcta tacataagtg gtgaccacgg acctggtgag cagggcgcgc
     3181 gctggcgaca gcgacgcgtt ccggaagctg acggagccgc accggcgaga gctgcaggtg
     3241 cactgttatc ggatgctggg atcgctgcag gacgccgagg atgccttgca ggagacgctg
     3301 ctgtccgcat ggcaaggcct cgacggcttc gagggacgcg catcgcttcg cacctggctc
     3361 taccggatcg ctaccaacag gtgtctcaac gcgcgccggt ccgcgagccg acgttcggcc
     3421 aaggagtggg acatgccagc tgtcgaacca ccagagccaa ccggattcgg tgaaatccct
     3481 tggcttgaac cgtatcccga tgccctcctc gacaaggcaa ccgatgtcgg tccc
//
LOCUS       DASRJA010000406        10606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1516730,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000406 DASRJA010000000
VERSION     DASRJA010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10606
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516730"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFC01_17730"
     CDS             complement(<1..838)
                     /locus_tag="VFC01_17730"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN81504.1"
                     /translation="MPARVVSREIEARAVGDFLSLVADEPSALVIEGEAGIGKTTLWL
                     AGLTQARDRGFTVLSTRPVAAESVLAYASLADLVGGLDAAVWDNLPTPQRFAMDGIMF
                     RADTDGAAIDHRAVAAGFLSAVEWQAEESPVLLSVDDLQWLDPSSAGVLAFAVRRLSG
                     PVGVFGTVRIEPDASDASWLQMPRPDAIRRIGVSPMSLGGLHAVISERLGRSFPRPAM
                     VWIHEVSGGNPFYALELARKVDTASSSSSEAQLPGTLADLVRTRIGHLDPDAKELLLA
                     AAC"
     gene            complement(851..1729)
                     /locus_tag="VFC01_17735"
     CDS             complement(851..1729)
                     /locus_tag="VFC01_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN81505.1"
                     /translation="MELRQLEYFVAVAEEANFTRAAERIHVAQPAVSAQIQRLEREVG
                     QPLLDRSRRTVRLTAAGEAVLPYARAALAAVADIHVAVEELTQLVRGSVTIGTVTSHS
                     VDIPSLLAAFHDEHPNVEITLSTDNSDALIEKVRTGQLDAAIVSVPPDEHPEGLDIEV
                     ATDEAIVAVVGPTDALASRKTIRLRDLADRQLIALPVGAGIRHQFDDACAKAGVTPRI
                     AFEASTPLALADLAERGLGVAIVPASVPRGRDGLHAVAIVPELRGRLALAWRSGGPMS
                     PAANVLVNKARRLLRS"
     gene            1800..2936
                     /locus_tag="VFC01_17740"
     CDS             1800..2936
                     /locus_tag="VFC01_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZN81506.1"
                     /translation="MTDVLIAGGGIGGLTAALTLHARGIEPTVLERASRLRPLGVGIN
                     LLPHAVSELYALGLGDELSSVAVAPTSISYYDTNGKLLFREPRGIDGGYGYPQYSVHR
                     GELQMLLLSALRERLGPNAVRTGAELHSFTETGRRVTAHTAAGDFTADALVGADGIHS
                     AVRAQLHSDHDPLLWSGVRMFRGAAPGDFLDGRTMVIVKGPDGVDLVTYPIGGGLVNW
                     VVMMPEADPGPLPGDAKWNQPGDRSQVLKHLERWRLGFLDAADLVRRTEPALEYPMVD
                     RDVLEWWGRGRVTLLGDAAHPMYPVGANGGSQAIVDARVLADELARDIDGGLRSYEDI
                     RRAETADVVKANREMHTTGDTQRPEDLARVTGKYRQDTHADTVR"
     gene            2936..3601
                     /locus_tag="VFC01_17745"
     CDS             2936..3601
                     /locus_tag="VFC01_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN81507.1"
                     /translation="MTTYVLVPGACHGPWCFDDLAEALRSRGHRVLPVALTGIAERAH
                     LLHAGVNLETHVADVLAELAVHDVSDAVLVGHSYGGMVITAVADRVPQQVDSLIYLDA
                     FVPRDGESCWTLTTDDQRAWYAGVDDTGYGVPPLPFFDARATAHPLASLMQPVRLTGD
                     LSRFRRRIYVYATKWDDESPFAATLERVRRDPSWTTHVLDGAHNLMRDCPDELLRILV
                     DAA"
     gene            complement(3598..4092)
                     /locus_tag="VFC01_17750"
     CDS             complement(3598..4092)
                     /locus_tag="VFC01_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81508.1"
                     /translation="MPNPFKDWARMYGANKQYLESHGRPSSFFGQIADIPNRIHEAAD
                     ASEIGVKMMRHSQLTNGAGLPATVTVEGVWPVGSYLNMSPVLRIQGRVEREDGTAPYG
                     AVFDEVVAQMHVARAQPGATLAVFVDPQNPVDMAIDWIRTGQLGPPPPGFIPGPGEPG
                     ARQV"
     gene            complement(4108..4488)
                     /locus_tag="VFC01_17755"
     CDS             complement(4108..4488)
                     /locus_tag="VFC01_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81509.1"
                     /translation="MGLFDKAKKAAQQAQQAFAQPQQGDGMVHVKGAGPIDTAMFGGP
                     STKSVAADDPIWQPINGISLQDYADLARDAQARGVTDEAGMIAIAQEKGWSPTDTKAA
                     LAGWVQRMGQSMAVGQQFRKFLGY"
     gene            4641..5081
                     /locus_tag="VFC01_17760"
     CDS             4641..5081
                     /locus_tag="VFC01_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZN81510.1"
                     /translation="MSAASTQPAIDGWFATDGSGEPYLIGGKCHQCGTYVFPPRANNC
                     PNPACDGDELAQVPLSRRGTLWSYTENRYAPPPPYPSPDPFEPFAVAAVQLADEGLIV
                     LGKVVEGTLAADLKVGMEMELTTMPLFVDDDGVERIVYAWRIAS"
     gene            5078..6277
                     /locus_tag="VFC01_17765"
     CDS             5078..6277
                     /locus_tag="VFC01_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HZN81511.1"
                     /translation="MSPEPLYILGAGMHPWGKWGNDFTEYGVAAARAALAEAGMDWRQ
                     VQLVAGADTIRNGYPGFVAGSTFAQKLGWNGVPVSSSYAACASGSQALQSARAQILAG
                     FCDVALVIGADTTPKGFFAPVGGERRNDPDWQRFHLIGATNTVYFALLARRRMDLYGA
                     TLEDFATVKVKNSRHGLNNPNARYRKEASVEDVLASPVVSDPLRLLDICATSDGAAAL
                     IVASAEFTKKHLGSVEGVPSVRAVSTVTPRYPQHLPELPDIATDSTAVVPAPDRVFKD
                     QILDAAYAEAGIGPEDLSLAEVYDLSTALELDWYEHLGLCAKGEAEQLLRSGATTIGG
                     RIPVNASGGLACFGEAIPAQAIAQVCELTWQLKGQATGRQVEGATVGVTANQGLFGHG
                     SSVIVAR"
     gene            6264..6950
                     /locus_tag="VFC01_17770"
     CDS             6264..6950
                     /locus_tag="VFC01_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HZN81512.1"
                     /translation="MSLAREFSAYGPSHWAVIAVFVIGAGLLVWVGRRQSERQARRLG
                     RILGAVTALIYAAILVYVLTPPTLDSVPLQLTDLATVVAAYALWSQKHWAYALTYYWG
                     LVLSTQALISPALRSPDFPHYQFLAFWAIHLLVVWAAIYLTWGRGMRPDWHSYRIAVA
                     VSAVWAVVTFTFNSFAGTNYGFINRKPSTASLLDVMGPWPWYVFIGTALIVTVWALMT
                     WPWERMRSSR"
     gene            6960..7277
                     /locus_tag="VFC01_17775"
     CDS             6960..7277
                     /locus_tag="VFC01_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81513.1"
                     /translation="MAIPDDWAPDACTLPTAERPLRVAEFNDLFTFVVRAQRQDPQRL
                     DLILRRIVEAPARDLARRESECCSFFTFEFESAGDDLVMHIAVPPEHVEVLDALEARA
                     TAS"
     gene            7361..7591
                     /locus_tag="VFC01_17780"
     CDS             7361..7591
                     /locus_tag="VFC01_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZN81514.1"
                     /translation="MADIISVRVKPGSRKGPLVDIGADGELTIHVREPAIEGKANDAV
                     VKLLAAHLGVPRRRLELISGATSRVKRFRIVH"
     gene            complement(7688..8428)
                     /locus_tag="VFC01_17785"
     CDS             complement(7688..8428)
                     /locus_tag="VFC01_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN81515.1"
                     /translation="MTSSDDRPILLEARDVVVHYGRIKALHGVSLVVHEGELVTMLGA
                     NGAGKTTMMRAISGLRPLTSGSVWFDGQEITGVKAHKRVTDGLIQAPEGRGVFPGMTV
                     TENLEMGCYGRKFASKAEHNERLDWVLETFPRLAERKNQVGGTLSGGEQQMLAIGRAL
                     MARPKVLLLDEPSMGLAPMVISQIFKIIAEINSQGTTVLLVEQNAQQALTRSDRAYIL
                     ETGEITREGNARELLADDSIRAAYLGVA"
     gene            complement(8415..9242)
                     /locus_tag="VFC01_17790"
     CDS             complement(8415..9242)
                     /locus_tag="VFC01_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN81516.1"
                     /translation="MTIEDLAGVHREIHAAEGETLLETKDLTVKFGGLVALDSVTFSI
                     RRGEILGLIGPNGAGKTTCFNAITGVYRPSSGTVIFDGAPLGRIKRHQITRKGIARTF
                     QNIRLWGEMTALENVVVATDARHHTSVPGALVRTPRHRREEKTAIERAAALLHFVGIA
                     HRGEEKAKNLPYGDQRRLEIARALATEPKLLCLDEPAAGFNPREKAALMDLIRKIRED
                     GYTVLLIEHDMRVVMGVTDRIVVLEFGRKIADGLPAEIREDPKVIAAYLGVPDDDVK"
     gene            complement(9239..10414)
                     /locus_tag="VFC01_17795"
     CDS             complement(9239..10414)
                     /locus_tag="VFC01_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN81517.1"
                     /translation="MSVTDKVLAPGDRLREWWDGLSRVQKWAFGILLFGALALLPLYT
                     PPFLDTPGISFGGTMAQFAMVAIIAIGLNVVVGQAGLLDLGYVGFYAVGAYTVALLTS
                     PNSPWNRLSPTGMFSAPWAWLSCVPLAMAMTALAGLILGIPTLRLRGDYLAIVTLGFG
                     EIIRLMADNLSDITNGPRGLSQVAFPRLGESEKLPEGVFSNGNSSGHANYGTWWFWLG
                     LVLIVAILLLVGNLERSRVCRAWVAIREDEDAAEVMGVNTFRFKLWAFVIGAAIGGLS
                     GALYAGQVQYVAPPSFNIINSMLFLCAVVLGGQGNKLGVIFGAFVIVYLPNRLLGVHF
                     LGINMGDLKYLFFGLALVVMMIFRPEGLFPARAQLLAYSKAARDWLRTPPVEKEPAR"
     gene            complement(10411..>10606)
                     /locus_tag="VFC01_17800"
     CDS             complement(10411..>10606)
                     /locus_tag="VFC01_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN81518.1"
                     /translation="AVLGGIGNLRGALLGGLLLGIMENYGQAVFGTQWRDVVAFVLLV
                     LVLLFRPTGILGESLGKARA"
BASE COUNT         1769 a         3528 c         3465 g         1844 t
ORIGIN      
        1 cacaggctgc ggccaatagg agttctttcg cgtcggggtc aaggtgtccg atcctcgtcc
       61 gcaccagatc ggcgagtgtg ccgggtaact gcgcctcgct actcgatgaa gatgcggtat
      121 ccaccttccg cgccaattcc agggcgtaga acgggtttcc acccgatacc tcgtgaatcc
      181 agaccatggc aggtcgcggg aacgaccggc cgagtcgctc ggagatcacc gcgtgcaggc
      241 cacccaaact cattggcgaa accccgattc gacggatcgc gtcgggacgc ggcatctgca
      301 accatgacgc atcggacgcg tcgggttcaa tgcgaaccgt gccgaacacc cccaccggac
      361 cagagagccg tcgcaccgcg aacgccaaca cgccggcgct cgacggatcc agccattgca
      421 gatcgtcgac ggaaagcagg accggcgatt cctccgcctg ccactcgacg gccgagagaa
      481 accccgcggc caccgcgcga tgatcgatgg cagcaccgtc ggtatcagcg cggaacatga
      541 tcccgtccat ggcgaatcgc tggggggtcg gcaggttgtc ccacaccgcg gcgtcgaggc
      601 cgccgaccag atcggccagc gacgcgtagg ccagcaccga ctccgccgcg acgggtcgcg
      661 tcgacaacac ggtgaagcca cggtcacgcg cctgggtcag cccggcaagc cacagcgtgg
      721 tcttgccgat cccagcctcg ccctcgatca ccaaagccga aggttcgtca gcgaccaacg
      781 ataggaagtc gccgactgcg cgagcctcga tctcacggct cacgacgcga gcgggcatgg
      841 ttgaacaatc tcacgaacgc aacaaccggc gagccttatt gacgagcacg ttcgccgctg
      901 ggctcattgg gccgcccgac cgccaagcca gcgccagtcg gccccgtaat tcaggcacta
      961 tcgccacggc atgcaatccg tcacggccac gcgggacgga ggcgggcacg atcgcgactc
     1021 ccaggcctcg ctcggcgagg tcggcgagcg ccagcggtgt gctggcctcg aatgcgatac
     1081 gcggagtcac tccggccttg gcgcacgcgt cgtcgaactg atgccggatg cccgccccga
     1141 cgggcagcgc gatgagctga cggtccgcca ggtccctcag tcggatggtc ttgcgagacg
     1201 ccagcgcgtc ggtgggacct accaccgcca cgatggcttc gtcggtggcg acttcgatgt
     1261 ccaggccctc ggggtgctcg tcgggtggga cggagacgat cgccgcgtcc agctgcccgg
     1321 tgcgcacctt ctcgataagt gcatcggaat tgtctgtgct gagcgtgatt tcgacgttcg
     1381 ggtgttcgtc gtggaaagcg gccagcagcg acggaatgtc gacgctgtgt gacgtcaccg
     1441 tgccgatggt gacgctgcca cgcaccaatt gcgtcaactc ctcgaccgcg acgtggatgt
     1501 cggcgacagc cgcgagcgcc gccctggcgt acggcagcac ggcctcgccc gcggcggtca
     1561 accgcacggt gcgacgcgac cggtccagca gtggctggcc gacctcccgt tccagccgct
     1621 ggatctgggc gctgaccgcc ggctgcgcga cgtggatgcg ctcagcggcg cgcgtgaagt
     1681 tggcctcttc ggcgacggcg acgaaatact cgagctgccg aagttccata actgatgatt
     1741 atagtttgtg gcccatctcg gtattggact tatagcttgt tgcggacgag cctggaggca
     1801 tgactgatgt actgattgcc ggcggcggga tcggcgggct gaccgccgca ttgacattgc
     1861 acgcccgcgg catcgaaccg accgtcctag aaagggcatc tcggctgcgg cccctgggcg
     1921 tcggcatcaa cctgttgcca catgcggtca gcgagctcta tgcgctcgga ctgggcgacg
     1981 agctgtcgtc ggtcgccgtc gcgcccacct cgatctccta ctacgacacc aacggcaagc
     2041 tgctgttccg cgaaccgcgc ggaatcgacg gcggatacgg ctatccacag tattcggtgc
     2101 atcgcggcga gctgcagatg ctgttgctgt ccgcgctgcg agaacggctg ggccccaatg
     2161 ctgttcgcac aggcgccgag ctgcacagct tcaccgaaac cggacgacgg gtaacggcgc
     2221 atacggcggc aggcgacttc actgcagacg cgctcgtcgg cgccgacggc atccattcgg
     2281 cggtgcgcgc gcaactgcat agcgatcatg acccgctgct gtggtcgggc gtgcggatgt
     2341 tcaggggcgc ggcgcccggc gacttcctag acggccgcac gatggtgatc gtcaagggtc
     2401 ccgacggtgt cgacctcgtc acgtacccga tcggcggcgg ccttgtcaac tgggtggtca
     2461 tgatgcccga agcagaccca gggccgctgc cgggcgacgc gaagtggaat caaccgggcg
     2521 accgctcaca ggtgctaaag catttggagc gttggcgttt gggcttcctg gatgccgccg
     2581 atctggtgcg ccgcaccgaa ccggcgttgg agtatccgat ggtcgaccgt gacgtccttg
     2641 aatggtgggg acgcgggcgg gtaaccctgc tcggcgatgc cgcccatccg atgtatcccg
     2701 ttggcgccaa cggcggttcg caggcgatcg tcgacgcgcg ggtgctcgcc gacgagctgg
     2761 cccgggacat cgacggcggc ttgcggtcct acgaggacat ccgtcgcgca gaaaccgcgg
     2821 acgtggtgaa ggcgaaccgc gagatgcaca ccaccggcga tacccaacgg ccggaggacc
     2881 tggcgcgagt gaccggcaag taccgccagg acacccacgc ggacacggtg cgctgatgac
     2941 gacctatgtt cttgtgcccg gcgcctgcca cgggccgtgg tgcttcgacg acctggccga
     3001 ggccctgcgg tcgcgtgggc accgggtgct tcctgtcgcg ttgacaggta tcgctgagcg
     3061 ggcgcacctg ctccacgcgg gcgtcaacct cgagacgcat gtcgccgacg tgctggccga
     3121 gttggccgtg cacgatgtca gcgacgccgt gctcgtcgga cacagctacg gcggcatggt
     3181 catcactgcg gtcgcggatc gtgttccgca gcaagtggat tcgctgatct atttggatgc
     3241 gttcgtgccg cgcgacggcg agtcgtgctg gacgctgacc accgacgacc agcgcgcgtg
     3301 gtacgccgga gtcgacgaca ccggctacgg cgtgccgccg ctgccgttct tcgatgctcg
     3361 cgccaccgcg cacccgttgg cgtcgctgat gcaacctgtc cggttgaccg gcgacttgtc
     3421 ccggtttcgc cgccgaatct atgtgtatgc gacgaaatgg gacgacgagt cgccgttcgc
     3481 ggcgacgttg gagcgggtgc ggcgcgatcc gtcgtggacc acgcacgtgc tcgacggcgc
     3541 ccacaacctg atgcgcgatt gtcccgatga gctgctgcga atcctggtcg acgccgctta
     3601 gacctgtcgc gcgcccggtt cgcccggacc cggtatgaaa ccgggcggcg gcggaccgag
     3661 ctgaccggtg cggatccagt cgatcgccat gtccactggg ttctgcgggt cgacgaacac
     3721 cgcgagcgtc gcgcccggct gggcccgcgc gacatgcatc tgcgccacga cttcgtcgaa
     3781 gacggcaccg tacggcgcgg tgccgtcttc gcgctcaacc ctcccctgga tccgcaacac
     3841 cggggacatg ttcaggtacg agccgaccgg ccagacgccc tcgacggtga cggtggccgg
     3901 caggcctgcg ccgttcgtca gctgcgaatg ccgcatcatc ttcaccccga tctcgctggc
     3961 gtcggccgcc tcgtggatcc ggttcgggat gtctgcgatc tggccgaaga acgatgacgg
     4021 gcgtccatgc gactcgaggt actgcttgtt ggcgccgtac atccgcgccc agtccttgaa
     4081 cgggttgggc atgtcgatca gtcccggtca atagccgagg aacttgcgga actgttgtcc
     4141 gacggccatc gactgaccca tgcgctgcac ccagccggcg agcgccgcct ttgtatccgt
     4201 cgggctccag cccttctcct gtgcgatggc gatcatgccg gcctcgtcag tgacaccgcg
     4261 ggcctgcgca tcccgagcaa ggtcggcgta gtcctgcagc gaaatcccgt tgatgggctg
     4321 ccagatcgga tcatccgccg ccactgactt ggtcgacggg ccgccgaaca tggccgtgtc
     4381 gatcgggccg gcgcccttga catgcaccat cccgtcaccc tgctgcggct gggcgaacgc
     4441 ctgctgtgcc tgctgcgctg ccttctttgc cttgtcgaac agtcccatgg tgtctccgta
     4501 tcttcggggc ccagacggta atgtccggga cgctactacc gcacaagtgc acacgggccc
     4561 gttcgctacc gatcccaact accgtccgat gaaactggaa cacgtttcag ttttgcggcc
     4621 agaatcggta cgctgaccgg gtgtcagccg catctaccca acccgccatt gacggttggt
     4681 tcgccacaga tggatctggt gagccgtatt tgatcggcgg taaatgtcat cagtgcggga
     4741 cgtatgtgtt cccgccgcgc gccaacaact gtcccaaccc tgcctgtgac ggcgatgagt
     4801 tggcgcaggt gccgttgtcg cggcgcggaa cgctgtggag ctacaccgaa aaccgctacg
     4861 ccccgccgcc gccgtatccg tccccggatc cgttcgagcc gttcgccgtt gccgccgtgc
     4921 aactggccga cgagggcttg atcgtgttgg gcaaggtggt cgagggcacg ttggccgcgg
     4981 atttgaaggt cggcatggag atggagctga ccaccatgcc gctcttcgtc gacgatgacg
     5041 gcgtcgagcg gatcgtctac gcgtggagga tcgcgtcatg agccccgaac cgctgtacat
     5101 cctcggtgcg ggcatgcacc catggggcaa gtggggcaac gacttcaccg agtacggggt
     5161 ggcggcggcg cgggccgccc tcgccgaggc gggtatggac tggcgccagg ttcaactcgt
     5221 tgccggcgcg gacacgatcc gcaacggcta tcccgggttc gtggccgggt ccacgtttgc
     5281 acagaagctg ggttggaacg gcgtgcccgt gtcgtcctcg tatgcggcat gcgccagcgg
     5341 gtctcaggcg ctgcagagcg cgcgcgccca gatcctcgcc gggttctgcg acgtcgcgct
     5401 ggtgatcggc gccgacacca cccccaaggg gttcttcgca ccggtcggcg gggaacgccg
     5461 caacgatccg gactggcagc gcttccatct gatcggcgcg accaacacgg tgtacttcgc
     5521 gctgctggcg cgccgccgga tggaccttta cggcgccacg ctcgaggact tcgccacggt
     5581 gaaggtgaag aactcccgtc acggcctgaa caaccccaat gcgcgctatc gcaaggaagc
     5641 ctcggtcgag gatgtgttgg ccagcccagt ggtgtcggat ccccttcggc tgctggacat
     5701 ttgcgcgacc tccgacggcg cggcggcgct gatcgtcgcc agcgctgagt tcacgaagaa
     5761 gcatctgggg tcggtcgagg gcgtgccgtc ggtgcgggcg gtgtccacgg tgacgccacg
     5821 ctacccgcag cacctgcccg aattgccgga tatcgcaacg gattccaccg cggtcgtgcc
     5881 cgcaccggat cgggtgttca aggaccagat cctcgatgcc gcttacgccg aggcgggcat
     5941 cggtcccgag gacctgagcc tggctgaggt ctacgacctg tccactgcgc tggagctgga
     6001 ctggtacgag cacctcgggc tgtgcgcgaa gggtgaggcc gagcagctgc tgcgcagcgg
     6061 cgcgacgacg atcgggggcc ggatcccggt caacgcgtcc ggcgggctgg cctgtttcgg
     6121 cgaggcgata cccgcccagg ccatcgctca ggtctgcgaa ctcacctggc agctgaaggg
     6181 ccaggccacc ggccgccagg tcgagggcgc caccgttggc gtcaccgcca accagggcct
     6241 gttcggccac ggttcgtcgg tcattgtcgc tcgctaggga gttctcggcc tacggcccgt
     6301 cgcattgggc cgtcatcgcg gtgttcgtga tcggcgcagg cctcctggtg tgggtcgggc
     6361 gcaggcagag cgaacggcag gcccgacggc tcggccgcat tctcggggcg gtgaccgctc
     6421 tgatctacgc cgcaatcctg gtttacgtgc ttaccccgcc gacgctcgac tcggtgccgc
     6481 tgcagctgac cgacctcgcg acggtcgtgg ctgcgtacgc cttgtggtcg cagaagcact
     6541 gggcgtacgc actcacctac tactgggggc tggtgctgag tacacaggcg ttgatatcgc
     6601 cggcactgcg aagccccgat ttcccgcact accaattcct cgcgttctgg gcgattcacc
     6661 tgctggtcgt ctgggcggct atctacctga cctggggtcg cggcatgcgg ccggactggc
     6721 acagctatcg cattgcggtc gcggtctcgg cggtgtgggc ggtggttacg ttcacgttca
     6781 acagtttcgc cgggacgaac tacggtttca tcaaccggaa gccgtcgacc gcgtcgctgc
     6841 tggatgtgat gggcccgtgg ccgtggtacg tcttcatcgg aacggcgctg atagtcaccg
     6901 tgtgggccct gatgacgtgg ccgtgggagc ggatgcggtc gtcgcgctag cctcgatcta
     6961 tggcgattcc cgatgattgg gcgccggacg cgtgcacgct accgacggcg gaacgacctc
     7021 tgcgtgtcgc ggaattcaac gacttgttca ccttcgtggt acgggctcag cgccaggacc
     7081 cgcaacgact ggacctcatc ctgcgacgca tcgtcgaggc tcccgcccgc gatctggccc
     7141 gacgcgaaag cgagtgctgc tcgttcttca cgttcgagtt cgaatcggct ggcgacgatt
     7201 tggtgatgca catcgcggtt ccaccggagc atgtcgaggt actcgacgcg cttgaagcgc
     7261 gggcaaccgc gagctgacca atcctcgcga gcgtgcgcgt ctgcacgtcg acacgccgcg
     7321 aatcggcggc agtttgcgca cgctcgtcgt tgaatgagcc atggccgaca tcatctcggt
     7381 gcgggtcaaa ccgggcagca ggaaaggtcc gctcgtcgac atcggcgcgg atggtgagtt
     7441 gacgatccac gtccgagagc cggcgatcga aggtaaggcg aacgatgccg tcgtcaagct
     7501 gcttgccgca catctaggcg tgccgcgccg gcggctggag ttgatatctg gtgcgacatc
     7561 gcgcgtcaag cggtttcgaa ttgtgcattg accgcacggt cgcggctcac gagcgcgcgc
     7621 aaaatgtacg caactaccgg cgtgttggtg tgcaaacacg cacacccgcg gtagtaggag
     7681 tcggagtcta ggcgacgccg aggtaggccg cgcgaatgct gtcgtcggcc aacaactctc
     7741 gcgcgttgcc ctccctggtg atttcaccag tttccaggat gtaggcccga tcggaccgcg
     7801 tcagcgcctg ctgtgcgttc tgctcgacca gcagcacggt ggtgccttgc gaattgatct
     7861 cggcgatgat cttgaagatc tgcgatatca ccatcggcgc gaggcccatc gacggctcgt
     7921 cgagcagcag tactttcgga cgggccatca acgcgcggcc gatggccagc atctgttgct
     7981 caccaccgga cagtgtgccg cccacttgat tcttgcgttc ggccagccgc gggaacgtct
     8041 cgagcaccca gtccaggcgc tcgttgtgct cggccttgga tgcgaatttg cgtccgtagc
     8101 aacccatttc caggttctcg gtgaccgtca tgccggggaa cacgccgcgt ccctcgggcg
     8161 cctggatcag gccgtcggtc acccgcttat gtgctttgac gccggtgatc tcttgaccgt
     8221 cgaaccacac tgagcccgac gtcaacgggc gcaggcccga aatcgcgcgc atcatcgtgg
     8281 ttttgccggc tccgttggcg ccgagcattg tcaccagctc gccttcgtgc accaccagcg
     8341 acactccgtg cagcgccttg atgcggccgt aatgcacgac cacatcgcga gcctcgagca
     8401 ggatcggccg gtcgtcactt gacgtcatcg tcgggcaccc ccaggtaggc ggcgatgacc
     8461 ttcgggtcct cgcggatctc ggctggcaga ccgtcagcga tcttgcggcc gaactccagc
     8521 accacgatac gatcggtgac ccccatcacc acccgcatgt cgtgctcaat gagcaacacg
     8581 gtatagccgt cttcgcggat cttgcggatc aggtccatca gcgcggcctt ttcgcggggg
     8641 ttgaacccgg ccgccggctc gtccagacac agcagcttcg gctcggtggc cagcgcacgg
     8701 gcgatctcca gacgccgctg gtcaccgtag ggcaggttct tggccttttc ctcgccgcgg
     8761 tgcgcgatgc cgacgaaatg cagcagcgcc gcggcccgct cgatcgccgt cttctcctcc
     8821 cggcgatggc gtggcgtgcg caccagcgca cccggcacgg acgtgtggtg ccgcgcgtcg
     8881 gtggcgacga cgacgttctc cagggccgtc atctcgcccc acagccggat gttctggaag
     8941 gttcgggcga tgcccttgcg ggtgatctgg tggcgtttga tccggcccag cggcgccccg
     9001 tcgaagatca ccgtgcccga actcggccgg tagacgcccg tgatcgcgtt gaagcaggtg
     9061 gtcttgcccg cgccgttggg tccgatcagg ccgaggatct cgccgcggcg gatgctgaac
     9121 gtaaccgaat ccagcgccac gagcccgccg aacttgacgg tcaaatcctt tgtctccaaa
     9181 agcgtttcgc cctcggcggc gtggatctcc cggtgtacgc ctgccaggtc ctcgatcgtc
     9241 atctggccgg ctccttctcc actggcggag ttcgcaacca gtcgcgcgca gctttgctgt
     9301 acgccaacag ttgcgcgcga gcggggaaca gaccctcggg acggaagatc atcatgacca
     9361 ctagggcgag gccgaagaac aggtacttca ggtcgcccat gttgataccc aggaagtgca
     9421 caccgagcag tcggttgggc aggtagacga tgacgaacgc gccgaagatc acgcccagct
     9481 tgttgccctg cccgccgagc accaccgcgc acaggaacaa catcgagttg atgatgttga
     9541 agctcggtgg cgcgacgtac tgcacctggc ccgcgtacag cgcgccggac aacccaccga
     9601 tggccgcccc gatcacgaac gcccacagct tgaaccggaa ggtgttcacg cccatcactt
     9661 ccgcggcgtc ctcgtcctcg cggatggcga cccacgcgcg gcacacccga cttcgttcaa
     9721 ggttgccgac cagcagcagg atggcgacaa tcagcacgag gccgagccag aaccaccaag
     9781 tgccgtaatt ggcgtgcccc gacgaattcc cgttggagaa cacaccttct ggaagtttct
     9841 cgctctcccc caaccgcggg aacgccacct ggctcagccc ccgcggaccg ttcgtgatgt
     9901 cggagagatt gtcggccatc agtcggatga tctcgccgaa tcccagcgtg acgatcgcca
     9961 gatagtcacc gcgcagacgt aatgtcggga tgccgaggat caggcccgcc agtgcggtca
    10021 tggccatcgc cagcggcaca cacgacaacc acgcccatgg cgcactgaac atcccggtgg
    10081 ggctgagccg gttccacggg ctgttggggc tggtgagcag cgcgacggtg tatgcgccga
    10141 cggcgtagaa gccgacgtaa cccaggtcca gcaagccggc ctggccgacc accacgttca
    10201 gcccgatcgc gatgatcgcg accatggcga actgggccat ggtgccgccg aagctgatcc
    10261 cgggggtgtc caggaacggc ggcgtgtaca gcggcagcag cgcgagcgcc ccgaacagca
    10321 ggatgccaaa ggcccacttc tgcacccgcg ataggccgtc ccaccactcc cgcagacggt
    10381 cgcccggcgc gagaaccttg tccgtcacgc tcatgcccgt gccttcccga ggctttcacc
    10441 gagtatcccg gtcggccgga acagcagcac gagaacgagc agtacgaatg ccaccacgtc
    10501 gcgccattgg gtgccgaaca cggcctgccc gtagttctcc atgatgccca gcagcagccc
    10561 accgagcagg gcgccgcgca gattgccgat gccgccgagc accgca
//
LOCUS       DASRJA010000407         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2289912,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000407 DASRJA010000000
VERSION     DASRJA010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289912"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /locus_tag="VFC01_17805"
     CDS             complement(<1..1899)
                     /locus_tag="VFC01_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN81519.1"
                     /translation="MAGGIASELAAAGFEEAVEVGRGGGGVVYRCYQKSLARNVAIKV
                     LASDLDEGDRERFLREGYAMGALSGHPNIVNILQVGVIESGRPFIVMSYHAVGSLAER
                     LRREGRIAWPEALHIGVKLCGALETAHRTGTLHRDIKPANVLFNDYGEPQLSDFGTAR
                     IAGGYKTVTGFFTGTLSYTAPEVLAGNPPTVAADVYSLGATFYALIAGAPPHERKTDE
                     DLIAHYLRITSTPVPDLRPHGIPADVCAAIEKAMSQEPGKRQASAAEFGRELQLAQRH
                     NGLTAEPMALSEPGGQPEHTGGTQALPLSGSDSPAESSEPASIAPGPPTAATPRPDPN
                     MFAHTASISQTSLPWQPVKPGPVPTAPPPKRNRNRILIASAAALAVLLLVIGGVFIVT
                     SSRDTGDGGGPPPQPTTEAEAPPAWRPIADAPAAGYGAAATEADGTIWIFGGMGAANR
                     VSGAHEGYDPAIDSWKSGDALPVPVQRAMSVTWQDTPTVLGGWRTEGANSKVATDRVW
                     RVVNSRWTELPPLMQPRAAAAAAVVGDRIIVTGGVDATGKLLNTTEIFDGTSWKLGAP
                     LPTPRQMSGAASDGKLVYVLGGTDGTSDLATVETYDPAADTWTSLPELPGRRSDVGVA
                     YADGRLVVV"
     gene            complement(2035..>2378)
                     /locus_tag="VFC01_17810"
     CDS             complement(2035..>2378)
                     /locus_tag="VFC01_17810"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930300.1"
                     /note="catalyzes the ATP-dependent formation of a
                     phosphodiester at the site of a single-strand break in
                     duplex DNA and has been shown to have polymerase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZN81520.1"
                     /translation="WRQGEGGRSSGIGALVLGIPEDGGLHFAGRVGTGFTEKELTKLK
                     GILKPLHTDESPFNTRLPTQDAKGVTFVRPELVGEVRYSERTSDGRLRQPSWRGLRTD
                     KTPDEVEWENS"
BASE COUNT          365 a          804 c          785 g          424 t
ORIGIN      
        1 caccaccaca agccgtccat cggcgtaggc cacaccgacg tcgctccgtc gccctggaag
       61 ctcgggcagg ctcgtccagg tgtccgcggc cgggtcatac gtctcgaccg tcgccaggtc
      121 ggaggtcccg tccgtcccgc cgaggacata caccagcttg ccgtctgacg ccgcgcccga
      181 catctgtcgc ggggtgggga ggggggcgcc gagcttccac gaggtgccgt cgaagatttc
      241 ggtggtgttc agcagcttgc cggtggcgtc cacaccgccg gtgacaatga tgcggtcgcc
      301 gaccacggcg gccgcagccg ccgcccgcgg ctgcatcagg ggtggcagct ccgtccagcg
      361 gctgttgaca acccgccaaa cccggtctgt cgcaaccttc gaattggcgc cttcggtgcg
      421 ccagccgccg agcacggttg gggtgtcctg ccaagtcacc gacatcgcgc gctgcacagg
      481 aacggggagg gcgtcgccac tcttccagct gtcaatggca gggtcgtagc cctcgtgcgc
      541 tccgctgacg cggttggccg cccccatccc gccgaagatc cagatggtgc cgtcggcctc
      601 ggtggccgcc gccccgtagc cggctgcggg cgcatccgcg atcggccgcc aagcaggcgg
      661 cgcttccgct tcggtggttg gttgaggtgg gggaccccca ccatcgccgg tgtcgcgcga
      721 tgacgtgacg atgaagacac cgccgatcac caacagcagc actgccagag cggcggcgga
      781 ggcgatcagg attctgttgc gattccgctt tggcggaggg gcggtgggca cgggaccggg
      841 cttgacgggt tgccacggca ggcttgtctg gctgatcgac gcggtgtgcg cgaacatgtt
      901 cggatccggc cggggcgttg ccgctgttgg tggccccggt gcgatggaag caggttcaga
      961 cgactcggcc ggactgtcag accccgacag cggtagcgct tgcgtgcccc ccgtgtgctc
     1021 gggttgaccg ccgggctcgc tgagcgccat cggctccgcc gtcaacccgt tgtggcgctg
     1081 cgccaactgc aattcgcggc cgaactccgc tgccgatgcc tgccgtttgc ctggctcctg
     1141 cgacatcgcc ttctcgatcg cagcgcacac atctgcgggg attccgtgcg gacgtagatc
     1201 cggcacgggc gtcgaggtga tgcgcagata gtgcgcgatg agatcctcgt cggttttgcg
     1261 ctcgtgcggc ggggccccgg cgatcagcgc gtaaaacgtg gcgccgaggg aatacacgtc
     1321 ggccgcgacg gtcggcggat tgccggcgag cacttcgggc gcggtgtaag acagggtgcc
     1381 ggtgaagaat cccgtcaccg ttttgtagcc acccgcgatg cgcgcggtcc cgaagtcgct
     1441 cagttggggt tcgccgtaat cgttgaaaag gacattcgcg ggcttgatat cgcggtgcag
     1501 cgtgcctgtc cgatgcgcgg tttccagcgc tccgcacaac ttcactccga tgtgcaatgc
     1561 ctcgggccac gcgatccgac cctcccggcg tagtcgctcc gcgagggaac ccactgcgtg
     1621 gtacgacatc acgatgaatg gccggccact ttcgatcacg ccgacctgca ggatgttcac
     1681 gatgttcggg tggccggaaa gcgcacccat tgcgtagccc tcacgcagaa aacgttcccg
     1741 gtcaccttcg tcgagatccg acgcaagcac cttgatggcg acgtttcgcg cgagagactt
     1801 ctggtagcag cggtagacaa ccccgccgcc gccgcggccc acctcgacgg cctcctcgaa
     1861 gccggcggcc gccagctcag acgctatccc gcccgccata gtgccaatcg tcctccgtaa
     1921 atcggacact ttgctcctaa ggtaccgagc aaaggcacca caagcggggg atttgcactt
     1981 cgttgccggg tcagcagctc ttcgaagccc tcaccggcgg cgggaccgta tttattacga
     2041 gttttcccac tcgacttcgt cgggcgtttt gtccgtccgc agtccgcgcc aactcggctg
     2101 acgcaggcgg ccgtccgatg tgcgttcgct gtagcgcacc tcgccgacga gttccggccg
     2161 gacgaacgtc acgcccttgg cgtcctgggt gggaagcctt gtgttgaacg gtgattcgtc
     2221 ggtatgcaga ggcttgagga tccccttgag cttggtgagc tccttctcgg tgaaacccgt
     2281 gccgacgcga cccgcgaaat gcagtccccc gtcctcgggt atgccgagca ccaaggcacc
     2341 gatgccgctg gaccggccac cctcgccttg ccgccagc
//
LOCUS       DASRJA010000408         9025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2736194,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000408 DASRJA010000000
VERSION     DASRJA010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2736194"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VFC01_17815"
     CDS             <1..1101
                     /locus_tag="VFC01_17815"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN81521.1"
                     /translation="YTAARIIARLVNEHQWRVGVVAQSHAVVENLFRDLIAAGVDPAR
                     IGKKDSPADAAWKHIDKDDYAAFIATHGGCVIGGTAWDFANDTRVDAGSLDLLVIEEA
                     GQFCLANTIAVARAANNLMLLGDPQQLPQVSQGHHPEPVDASALGWLVDGRRTLPEEF
                     GYFLDRSYRMHPAVCEAVSRLSYEGRLHSEEGRTAARRLDGYEPGVRVLTVDHDGNST
                     SSPEEATAIVAEAQRLLGSQWTDENGTRSLGQDDVLVVAPYNAQVLLLQDYFEAAGLP
                     DIAVGTVDKFQGRQAPVVFVSMTASSIDDVPRGISFLLNRNRLNVAVSRAKYLSVIVR
                     SEALTEYLPATPDRLVELGAFLSLAPCDTPSE"
     gene            1098..2444
                     /locus_tag="VFC01_17820"
     CDS             1098..2444
                     /locus_tag="VFC01_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN81522.1"
                     /translation="MSLTDTLAGIVGAAHVVTDPDVLDGRSIDHTGRYRGHASALVRP
                     GSADEVAAVLRACRDAGSFVTVQGGRTSLVAGTVPEHDDVLLSTERLHDVGEVDVIER
                     RLSVGAGVTLGEVQRAAVKAGLVFGVDLAARDSATVGGMASTNAGGLRTVRYGNMGEQ
                     VIGLDVALPDGSVVHRHSQVRRDNTGYDLAALFIGAEGTLGVITALDLRLHPTPTHRV
                     TAICGFDDLDALVETGRVFRDMDGIAALELIDARASALTAEHVGVAAPVDGAWQLLIE
                     LASDTDQTEQLADALEAARMSDEPAVGVDVSAQQRLWQVRESVAEVLGVYGPPLKFDV
                     SLPLSAIAGFASESAELVAKHAPDAIPVLFGHIGEGNLHLNVVRCGPDAERELYAPMM
                     ELIARSSGNVSSEHGVGTRKRDYVSMSRTQPDIAAMRAVKAAFDPSGYLNPAVLFG"
     gene            complement(2441..4513)
                     /locus_tag="VFC01_17825"
     CDS             complement(2441..4513)
                     /locus_tag="VFC01_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN81523.1"
                     /translation="MLVGFFMILVDATIVAVANPSIMDKLGASYDSVIWVTSAYLLAY
                     AVPLLVAGRLGDRFGPKNLYLLGLTVFTAASLWCGLAGSIGMLVAARVVQGIGAALLT
                     PQTLSTITRIFPADRRGVAMSVWGATAGVATLVGPLAGGVLVDHLGWQWIFFVNVPIG
                     IIGLALAVWLIPTLPTSTQRFDLIGVVLSGVGMFMIVFALQEGQSHHWAPWIWGTLAG
                     GIGFMAAFVIWQSVNRNDPLIPLVIFRDRDFSLSNLGIATIGFVVTGMILPVMFYAQA
                     VCGLSPTRAALLTAPMAIATGVLAPFVGRIVDRSHPRPVIGFGFSVLAVALTWLSIEM
                     TPTTPIWRLVLPLTGMGVGMAFIWSPLAATATRNLPGRLAGAGSGVYNTTRQVGAVVG
                     SAGMAAFMTWRISSEMPPMPMQAQREGSVTQLPAWLHEPFAAAMSESLLLPAFFALFG
                     VVAAMFLLGFGNPKRLSEDDDLNAADRNARYAVEYGGDETFVDDDEYVEYVVSWDDDP
                     EPATQVEPATPAEPAASVEPVEPADDTVTEPMQSRAEHLLHAPAEAWHSDPVESWHSL
                     LDEEPEQPPAEPPAPEPPAETQHEPWRGILDELMSDIPPLKPKAEPIGFAHNGFHVDE
                     EQRFQPMPPPAPEQRRPSRHERSARHHRTENEPQRRPFWFEANGRHSRDDPDDASRSG
                     RHSMPGRD"
     gene            complement(4697..5983)
                     /locus_tag="VFC01_17830"
     CDS             complement(4697..5983)
                     /locus_tag="VFC01_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HZN81524.1"
                     /translation="MAASTDVDVTRAEISTPDGVSKPPSRVRMIAVLGALVALGPLTI
                     DMYLPALPKIADDLSVSSSVAQLTLTGTLAGLALGQLIVGPLSDSLGRRRPMMAGIVL
                     HMLASMLCLFAPNIAMLGVARGLQGMGAAAAMVVAIAVVGDLYAESVAATVMSRLMLV
                     LGVAPVIAPSLGAAVLLKASWHWVFAALVVLAGGLLLLATLALPETLPASHRRPLKVR
                     SIAATYVELLRDARFVILVLVAALGMSGLFAYIAGASFVLQGHYGLDQQAFALVFGAG
                     AVALIGATQFNVVLLRRFSPQTIVLWALVAAVVAGVVFVGLSYEHVGGLAGFVVPVWA
                     ILAAMGLVIPNAPAVALSRHPDAAGTAAALLGAAQFGLGAAVAPLVGVLGNNEIALAL
                     VMTVGMVIALLALLAVGVPATETKDDVTGDAVAEAA"
     gene            6126..6872
                     /locus_tag="VFC01_17835"
     CDS             6126..6872
                     /locus_tag="VFC01_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81525.1"
                     /translation="MSARSNTKSQLPATTDESPGAAAKVLSQILERGTRVQGPAIKAY
                     VDRLRQQNPDATPADIAKKLEKHYLAAVVASGAAVGSAAAFPGIGTLTALSAVAGETM
                     VFLEATAVYVLSLAEVYGIPADHRERRRALVLAVLVGEDSKRAVADLLGPGRTSGAWI
                     SDGAATLPLPAVSQLNSRLLRYFVKRYTLKRGAIAFGKLLPVGIGAVVGGVGNRMMGK
                     KMVANARAAFGNPPPRWPASLHVLPAPSER"
     gene            7071..>9025
                     /locus_tag="VFC01_17840"
     CDS             7071..>9025
                     /locus_tag="VFC01_17840"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HZN81526.1"
                     /translation="MYRKFREDPSSVDPSWHEFLVDYSPEPTTEAQSTTGNGHPAQVA
                     APVAPPEPAPAPAPKAETAPAKAPEPQKAAEPEKAPAKPKAPAAPAPAEGDELQVLRG
                     AAAAVVKNMSSSLEVPTATSVRAIPAKLMIDNRVVINNHLKRTRGGKISFTHLIGYAL
                     VQAVKKFPNMNRHFAEIDGKPNAVTPEHVNLGLAIDLQGKDGNRQLVVAAIKGCETMT
                     FGQFIAGYEDIVRRARDGKLTAEDFSGVTISLTNPGTIGTVHSVPRLMRGQGAILGVG
                     AMEYPAEFQGASEERISELGVGKLVTFTSTYDHRIIQGAESGDFLRTIHELLLSDDFF
                     DEIFRELGIPYEPVRWRTDNPDSIEDKNGRVLELIAAYRNRGHLMADIDPLRLDKTRF
                     RSHPDLDVNTHGLTLWDLDREFKVNGFAGAEHKKLRDVLGLLRDAYCRHVGVEYTHIL
                     EPEQQKWLQERIEVKHDKPTVAQQKYILSKMNAAEAFETFLQTKYVGQKRFSLEGAET
                     VIPMMDAAIDQAAEHALDEVVIGMPHRGRLNVLANIVGKPYSQIFSEFEGNLNPSQAH
                     GSGDVKYHLGATGNYIQMFGDNDIQVSLVANPSHLEAVDPVLEGLVRAKQDLLDKGDG
                     PDGFSVVPMMLHGDAAFAGQGVVAETL"
BASE COUNT         1636 a         3028 c         3020 g         1341 t
ORIGIN      
        1 tacacggccg ccaggatcat cgccaggctg gtcaacgagc accagtggcg ggtcggcgtc
       61 gtcgcgcagt cacatgcagt ggtggagaac ctgtttcgcg atctgatcgc ggccggcgtc
      121 gaccccgcgc ggatcggcaa gaaggattcg ccggcggatg cggcatggaa gcacatcgac
      181 aaggacgact acgcggcgtt catcgccacc cacggcggct gcgtaatcgg cggcaccgca
      241 tgggatttcg cgaacgacac ccgggttgac gcgggcagcc tcgatctgtt ggtcatcgag
      301 gaggccggcc agttctgcct cgcgaacacc atcgccgtgg cgcgcgccgc gaacaacctc
      361 atgctgctcg gcgaccctca gcagctgccg caggtcagcc agggccatca tccagaaccg
      421 gtcgacgcgt cggctctggg ctggcttgtc gacggccgcc ggacgctgcc cgaagagttc
      481 ggctacttcc tcgaccggtc ctaccggatg catccggcag tgtgcgaagc cgtgtcgagg
      541 ctgtcctacg agggccggct gcactccgag gaaggccgaa ccgcagcacg caggctggac
      601 gggtacgagc cgggcgtgcg ggtgctgacg gtcgatcacg acggcaactc gaccagcagc
      661 ccggaggaag cgacagcgat cgtggccgag gcgcagcgcc tgctcggttc acagtggacc
      721 gacgagaacg gcacgcggtc cctcggacaa gacgatgtgc tcgtggtcgc gccctacaac
      781 gcccaggtgc tgctgctgca ggactatttc gaagccgccg ggctgcccga catcgccgtc
      841 gggaccgtcg acaagttcca gggccggcag gccccggtgg tgtttgtgtc gatgacggca
      901 tcatcaatcg atgacgttcc gcgcggcatt tcgttcctgc tgaacaggaa tcgtctcaac
      961 gtcgcggtca gccgcgccaa gtatctgagc gtcatcgtcc gctcggaggc gctgaccgaa
     1021 tacctgccgg ccactccgga ccggctcgtg gagctgggcg cgttcttgtc gctggcgccg
     1081 tgtgatacac cgtcggaatg agcctgaccg acacgctggc cgggatcgtg ggtgccgcgc
     1141 acgtcgtcac cgatcccgat gtgctggacg gccgcagcat cgaccacacc ggccgctatc
     1201 gcgggcacgc cagcgcgctg gtgcggccgg ggtcggctga cgaagtcgcg gccgtgctgc
     1261 gcgcctgccg cgacgcaggc tcattcgtca ccgtgcaggg cgggcggacc tcgttggtgg
     1321 cagggacagt gcccgagcac gacgacgtgc tgttgtcgac cgagcggcta cacgacgtcg
     1381 gcgaggtcga cgtgatcgag cggcgactat cggtgggcgc gggcgtgacc ctcggcgagg
     1441 tgcagcgggc ggcggtcaag gccggtttgg tgttcggcgt cgatctggcc gcgcgtgatt
     1501 cggcgaccgt cggcgggatg gcatcgacga acgccggcgg actgcgcacg gttcgatacg
     1561 ggaacatggg cgagcaggtg atcggcctcg acgtcgcgct gcccgacggg tcagtcgtgc
     1621 accggcacag tcaggttcgg cgcgacaaca ccggatacga cctggccgcg ctgttcattg
     1681 gcgccgaggg cacgctgggc gtgatcaccg cgctggatct gcggctgcac ccgaccccga
     1741 cgcaccgagt caccgcgatc tgcgggttcg acgacctcga cgcactggtc gaaaccgggc
     1801 gcgtgttcag ggacatggac ggcatcgcgg cgttggagtt gatcgacgca cgcgccagcg
     1861 cgctgaccgc ggagcatgtc ggggtggccg cgcccgtcga cggcgcatgg cagctgctca
     1921 tcgaattggc cagtgacaca gaccaaaccg aacagctggc cgatgcactc gaggctgcgc
     1981 gcatgtcgga tgagcccgcg gtcggcgtcg acgtctccgc gcagcagcga ctctggcagg
     2041 tccgcgaatc ggtcgccgaa gtgctgggtg tgtacggacc gccgctgaaa ttcgatgtat
     2101 ccctgccatt gtcggcgatc gccgggttcg catcggaatc cgccgagctg gtggcaaagc
     2161 atgcccccga cgcgatcccg gtgctgttcg gtcacatcgg cgaaggcaac ctgcacctca
     2221 acgtggtgcg gtgcgggccc gacgcggagc gcgaactgta cgcgcccatg atggagctga
     2281 tcgcccgcag cagcggcaac gtcagctccg aacacggcgt cggcacgcgc aagcgtgact
     2341 acgtgtcgat gtcgcgcaca cagcccgata tcgcggcgat gcgcgcggtc aaggccgcgt
     2401 tcgatccgtc cggatacctc aaccccgcag tgctgttcgg ctagtcgcgg cctggcatcg
     2461 agtgtctgcc ggacctcgac gcatcgtcgg ggtcgtcgcg ggagtgcctg ccgttggcct
     2521 cgaaccagaa cgggcgcctc tgcggctcgt tttcggttcg atggtgccgc gcagagcgct
     2581 catggcgtga cggcctccgc tgttctggcg ccggcggggg cattggctga aagcgttgtt
     2641 cctcatcgac gtgaaacccg ttgtgcgcga atccgattgg ctcagccttc ggtttgaggg
     2701 gcggaatgtc gctcatcaac tcgtcgagaa tacctcgcca aggctcgtgc tgtgtctctg
     2761 ctggcggctc cggcgcgggc ggctcggctg gcggttgctc gggctcctcg tcgagcagtg
     2821 agtgccacga ctccaccgga tccgagtgcc aagcctcggc cggcgcgtgc agcagatgct
     2881 cggcgcgcga ctgcatcggc tcggtcacgg tgtcgtcggc tggctcgaca ggctcgacag
     2941 acgcggccgg ctcggccggt gtcgcgggct cgacctgtgt cgccggttcg gggtcgtcgt
     3001 cccacgacac cacgtactcg acgtactcgt catcgtcgac gaacgtctca tcgccgccgt
     3061 attccaccgc gtagcgggcg ttgcggtccg ccgcgttcaa gtcgtcgtcc tcgctgagcc
     3121 gcttcggatt gccgaagccg agcaggaaca tcgcggcgac aacaccgaac aacgcgaaaa
     3181 acgcgggtaa cagcagcgac tccgacatcg ccgcggcgaa cggttcgtgc agccatgcgg
     3241 gcaactgcgt caccgaacct tcgcgctgcg cctgcatcgg catgggcggc atctccgagc
     3301 tgatccgcca cgtcatgaac gccgccatgc cggcgctgcc gaccaccgcg cccacctgcc
     3361 gggtggtgtt gtagacgccg gaaccggcgc cggccagtct ccccggcagg tttcgggtgg
     3421 ccgtggcggc cagcggcgac cagatgaacg ccatgccgac gcccatcccg gtcaatggca
     3481 agaccagccg ccagatcggc gtcgtcgggg tcatctcgat ggacagccag gtcaacgcga
     3541 ccgccaggac ggagaagccg aagccgatca ccgggcgcgg atgcgatcgg tcgacgatcc
     3601 tgccgacgaa gggggccaac acgcccgtcg cgatcgccat cggtgcggtc agcagcgcgg
     3661 cacgtgtcgg cgaaagcccg cacaccgctt gcgcgtagaa catgaccggc aggatcatcc
     3721 cggtcacgac gaagccgatg gtcgcgatgc cgaggttgga cagtgagaag tcgcgatcgc
     3781 ggaagatgac caacgggatc agcggatcgt tgcggttgac cgactgccag atcacgaacg
     3841 cggccatgaa gccgatgccg cccgcgaggg tgccccaaat ccacggcgcc caatggtgcg
     3901 actgaccttc ctgcagcgcg aacacgatca tgaacatgcc aacgccggag agcaccacac
     3961 cgattaggtc gaatcgctgt gtggacgtcg gcagcgtcgg gatcagccac acggccagcg
     4021 ccagcccgat gatgccgata ggaacgttga cgaagaagat ccactgccac ccgaggtggt
     4081 caaccagcac gccgccggcg agcggtccga ccagggttgc gacccccgcc gtggcgcccc
     4141 acacgctcat cgccacgccg cggcggtcgg ccgggaagat ccgcgtgatg gtcgacagcg
     4201 tctgcggcgt cagcaaggcc gcgccgatgc cctgcacgac gcgcgccgca accagcatcc
     4261 cgatgctgcc ggccaggccg caccacagcg acgcggcggt gaacaccgtc aagcccaaca
     4321 gatacaggtt cttcggcccg aaccgatcgc cgaggcggcc ggcgaccagc agcggcactg
     4381 catacgcgag taggtaggca ctggtcaccc agatgacgga gtcgtagctc gcgccgagct
     4441 tgtccatgat cgacgggttg gcgaccgcga cgatggtcgc gtcgaccagg atcatgaaga
     4501 agccgacaag catcgcccac aacgcgttcc aggggttggt ggcgtcagct ttcggccgcg
     4561 ctccccctcg agcgcggcca tcttgccggg tagtcagcgt ttggtacatc tctggtcgac
     4621 caggtaaaca acgattcagc cgacggtacg gtcctaggaa tgccccgaca agtccgggga
     4681 caaccagtgc gaaacgtcag gctgcttcgg cgacggcgtc gccggtgacg tcgtccttgg
     4741 tttcggtagc cggcacgccg accgccagta atgccagcag tgcgatcacc atgccgaccg
     4801 tcatcaccaa ggctaacgcg atctcgttgt tgccgagcac gccgaccaac ggcgccaccg
     4861 cggcacccaa gccgaactgc gccgcaccaa gcaacgccgc cgccgtgccc gcagcgtcgg
     4921 gatgccggga caacgccacg gctggggcgt tgggaatgac caggcccatc gcggccagga
     4981 tcgcccagac cggcaccacg aagcccgcta gcccgccgac gtgttcatag gacaggccga
     5041 caaagaccac acccgcgacg acggccgcta ccagcgccca cagcacgatc gtctgcggtg
     5101 agaaccgtcg cagcagcacc acgttgaact gggtcgcacc gatcagcgcg acggcgccgg
     5161 cgccgaacac cagcgcgaag gcctgctggt ccaagccgta gtggccctgc agcacgaacg
     5221 acgcccccgc gatgtaggcg aacaggcccg acatgccgag cgcggccacc agcaccaaga
     5281 tgacgaaccg cgcgtcgcgc agcagctcga cgtaggtcgc ggcgatgctg cgcaccttca
     5341 gcggccgccg atgcgacgca ggcagggttt ccggcagcgc cagggtcgcc agaagaagca
     5401 ggccacccgc caacacgacc agcgcggcga acacccagtg ccacgacgct ttcaacagca
     5461 ccgcggcacc caacgaggga gcgatcaccg gcgcgacgcc gagcaccagc atcagccgcg
     5521 acatcacggt ggcggccacg gactcggcgt acaggtcacc caccactgcg atggcgacga
     5581 ccatcgccgc ggcggcgccc atgccctgca gcccgcgcgc cacgcccagc atggcgatgt
     5641 tcggcgcgaa caggcacaac atcgacgcca gcatgtgcag cacgatgccg gccatcatcg
     5701 gcctgcggcg gcccagcgag tccgagagtg gcccgacgat cagctggccg agggccaatc
     5761 cggcgagggt gccggtcagc gtgagttgcg cgaccgacga ggacaccgac aggtcgtcgg
     5821 cgattttggg cagcgcaggc agatacatgt cgatcgtgag tgggcccagc gcgacaaggg
     5881 cgccgaggac cgcgatcatg cggacgcggc tcggcggctt cgacaccccg tccggggtag
     5941 agatttcggc cctggtcacg tccacgtcgg tcgatgctgc catggaagtg gttagcgccg
     6001 cttctcaata atttgttccc tgcctcacag acagtgaccc ctatcaccgc cgggcacccg
     6061 cgggccgccg tcgttcgtta acaacgcgtt agcctgaagg cagtcgctga cgaggggaac
     6121 cgcatatgag cgctcggtcg aacaccaaga gccagttgcc ggctaccacc gacgagagtc
     6181 ctggcgccgc agccaaggtg ctgtcgcaga tcctcgaacg cggcacccgc gtgcaggggc
     6241 cagcgatcaa ggcctacgtc gaccggctgc gtcagcagaa tcccgacgcg acaccggccg
     6301 acatcgcaaa gaagctggag aagcactacc tggccgccgt ggtggccagc ggcgccgcgg
     6361 tgggatccgc cgccgcgttc cccggcatcg gcacgctgac ggcgctgtcg gccgtcgccg
     6421 gcgagacgat ggtgttcctg gaggccacag cggtttacgt gctctcgctc gccgaggtgt
     6481 acggcattcc tgccgaccac cgcgagcgtc gtcgcgcttt ggtgctggcc gtcctggtcg
     6541 gcgaggacag taagcgcgcg gtggcggacc tgctcggacc cggacgaacc agcggtgctt
     6601 ggatcagtga cggcgcggca acgttgccgc tgccggcggt ctcgcagttg aattcccggc
     6661 tgctgcgcta tttcgtcaag cgctacacgc tcaagcgtgg ggcgatcgcg ttcggcaagt
     6721 tgctgccagt cggcattggc gcggtcgtcg gcggggtggg gaaccggatg atgggtaaaa
     6781 agatggtcgc caacgcgcgc gcggcgttcg gcaatccgcc gccgcggtgg ccggcctcgc
     6841 tgcacgtgct gcccgcccct agcgagcggt gaggataact gccgttcgca tggtttggcc
     6901 gacggccgaa gcgttagcct ttaggcggcg gataagtggg tgaccgcgga ccgcaggaat
     6961 atgggtggcg agcgtctgaa aagccgcttg cctgaagcga agaatcgagg cgagtagctg
     7021 ccgtgagctc accatcacca ttcggacaga acgagtggtt ggtcgaggag atgtatcgca
     7081 aatttcgcga ggatccctcg tcggtagacc cgagttggca cgaattttta gtcgactact
     7141 cacccgagcc aaccaccgaa gcgcaatcca ctacaggcaa cgggcacccg gctcaggtcg
     7201 ccgccccggt cgccccgccg gaaccggcgc ctgcacccgc accgaaggcg gagactgccc
     7261 ccgctaaggc acccgagccg cagaaggcgg cggaaccgga gaaggcgccc gccaagccga
     7321 aagcccctgc cgcgccggca cccgcagaag gcgacgaact gcaggtgctg cgtggtgctg
     7381 cggccgcggt cgtgaagaac atgtcctcgt cactcgaggt accgacggcg accagcgtgc
     7441 gggccatccc ggcgaagctg atgatcgaca accgagtcgt catcaacaat cacctcaagc
     7501 gcacccgcgg cggcaagatc tccttcacgc acctgatcgg gtacgcattg gtgcaggcgg
     7561 tcaagaagtt cccgaacatg aaccgccact tcgccgaaat cgacggcaag ccgaacgcgg
     7621 tcacgccgga gcatgtcaac ctcgggctcg caatcgacct gcagggcaag gacggcaacc
     7681 gccaactcgt ggtcgcagcc atcaagggtt gcgagacaat gactttcggc cagttcatcg
     7741 ccggctacga ggacatcgtc cggcgggccc gtgacggcaa gctgacggcc gaggactttt
     7801 ccggcgtgac gatctccctc accaacccgg gcacgatcgg caccgtgcac tcggtcccgc
     7861 ggctgatgcg aggtcagggc gcgatcctcg gcgtcggtgc gatggagtac ccggccgagt
     7921 tccagggcgc cagtgaggaa cgcatctccg agttgggcgt cggcaagctg gtcacgttca
     7981 cctccaccta cgaccaccgc atcatccagg gcgcggaatc gggcgacttc ctgcgcacga
     8041 tccacgaatt gctgctgtcc gacgacttct tcgacgagat cttccgcgag ctgggcatcc
     8101 cgtacgagcc tgtgcgctgg cgcaccgaca accccgactc gatcgaggac aagaacggcc
     8161 gcgtcctcga gctgatcgcg gcgtaccgca accgcggcca tctgatggcc gacatcgacc
     8221 cgctgcggct ggacaagacc cggttccgca gccaccccga cctcgatgtg aacacgcacg
     8281 gcctgacgct gtgggacctc gatcgcgagt tcaaggtcaa cggcttcgcc ggcgccgagc
     8341 acaagaagct gcgcgacgtg ctgggtctgc tgcgcgacgc gtactgccgc cacgtcggcg
     8401 tcgaatacac gcacatcctc gaacccgaac agcagaagtg gttgcaggag cggatcgagg
     8461 tcaagcacga caagccgacg gtcgcacagc aaaagtacat cctgtccaag atgaatgctg
     8521 ccgaggcgtt cgagacgttc cttcagacga aatacgttgg gcagaagcgg ttctcgcttg
     8581 agggtgcgga gaccgtcatc cccatgatgg acgccgcgat cgaccaggcc gccgagcacg
     8641 cgctcgacga ggtcgtgatc ggcatgcccc accgcgggcg gctcaacgtg ctggccaaca
     8701 tcgttggcaa gccgtactcg cagatcttca gcgagttcga gggcaacttg aacccgtcgc
     8761 aggcgcacgg ctccggcgac gtgaagtacc acttgggcgc gacgggcaac tacatccaga
     8821 tgttcggcga caacgacata caggtgtcgc tggtcgccaa cccgtcgcac ctggaggccg
     8881 tcgacccggt actggagggt ctggtccgcg ccaagcagga cctgctggac aagggcgatg
     8941 gcccggacgg cttctccgtc gttccgatga tgctgcacgg cgacgccgcg ttcgccggcc
     9001 agggcgtggt ggccgagacg ctgaa
//
LOCUS       DASRJA010000409         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3491482,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000409 DASRJA010000000
VERSION     DASRJA010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3491482"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..1188
                     /locus_tag="VFC01_17845"
     CDS             394..1188
                     /locus_tag="VFC01_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZN81527.1"
                     /translation="MDQPITASPVELTAAIPPDPAEELPPATRPPGSDLYDVMSTMRA
                     MRRLRPEPVPRELIERLIQAASWAPSGGNSQTTRYIVVDDRTQIARIAPLWRRVQGFY
                     VTAQADACPPTMSPKQWQRTLGASQYLADHFEDVPALIVVCCDMSSIMHSWLANAATV
                     ARAFGKLGIRRGATGGANFGKFARLSTAASVYPAVQNLLLAARQSGLGASLTTWHAMY
                     ERELKGILGIPRNVRTFAVVPIGYPIGRFGPVNRAPVDHVLRWNHW"
     gene            1264..>2817
                     /locus_tag="VFC01_17850"
     CDS             1264..>2817
                     /locus_tag="VFC01_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN81528.1"
                     /translation="MKSLLLPRRDIDFLLFEWLRVDELTKRERFADHSTESFGDVLAL
                     CEQLAEQCFAPHNKLADVHEPVFDGTNVTVLPEVKDAFGAFSATGLLGMTMDEKHGGA
                     QFPVVVATAAYLWLQAANFSTISYSLLSLANANLLASFGSAEQTETFLSPILSGRYTG
                     TMCLSEPHAGSSLADITTRAEPEDSGAYRLFGSKMWISGGDHDITENIVHLVLAKLPG
                     APAGTKGISLFIVPKHLVNADGSIGERNDVVTAGLNHKMGQHGMTNALLNFGDGAFRP
                     GGRAGAVGYLVGQPHHGLAYMFHMMNEARLMAGAAATALGYTAHLKSLEYARTRTQGR
                     PITSKDPATPPVPIIEHPDVKRMLLAQKSYVEGALALVLYCSRLVDIASSAESREEAE
                     SATLLLDLLTPVAKSWPSQWCLEANDLAIQLHGGYGYSREYDVEQHYRDNRINAIHEG
                     THGIQSLDLLGRKVRQRDGAGLRLLAGEIGRTIAEADLLGGEPEQLARQLDWAWRRLS
                     DTTATLLRCD"
BASE COUNT          553 a          922 c          833 g          509 t
ORIGIN      
        1 gcggtgcggc gagcgtatag gcaccgccga gtaaggcgcc ccgccttcac gggctgctta
       61 cccatcaccg ggcaacgtca ccgccgaacc ctgtctgcgg agggagtcct gaagctatgt
      121 ccgaatacct ctctggcgta atcattttca tgctagtgtt ctcgccgctg ttcataccga
      181 tagctgtcac catcatcgat gctgtgcgcc gctggtcgag cttggctgcc cactcctccc
      241 gaacgcatcg aagtaggtcg tctgatgacc ttccgccgaa gcccgctgcg accgccggcg
      301 ccgggacgga cccggtgtca atcggatacc tagcacggcc agttcctgag ccctcctaag
      361 tcaccaccga ccaagacgag gagaaactga atagtggatc agcccatcac tgcgtcgccc
      421 gttgagctca cggcagctat cccgccggac ccggcggaag aactgcctcc agcgacgcgg
      481 ccgccgggca gtgatctgta tgacgtcatg agcacgatgc gcgcgatgcg ccgactcaga
      541 ccggagccgg tgccgcgtga gctgatcgaa cggctgatcc aggctgcgtc gtgggcgccc
      601 agcggcggca actcgcaaac cactcgctac atcgtggttg acgaccgaac ccaaatcgca
      661 cgaattgcgc cgctgtggag gcgagttcaa ggcttttacg tcaccgcgca ggccgacgcg
      721 tgtccaccga caatgtcacc gaagcagtgg cagcgcacgt tgggggcgtc gcagtatctc
      781 gccgaccact tcgaagacgt gcccgcgctg atcgtggtgt gctgtgatat gtcgtcgata
      841 atgcacagtt ggctcgccaa tgctgcgacg gtcgcacgtg ctttcggcaa gctaggtatc
      901 cgccgaggcg cgacgggtgg ggcgaacttc ggcaagttcg cacgcctatc caccgccgcc
      961 agcgtctacc cagcggttca aaatttgctc ctggccgccc gccagtccgg cctgggtgcc
     1021 agtctgacca cctggcatgc gatgtacgag cgggaactca aaggcatact cgggataccg
     1081 cgcaatgtcc gcacattcgc cgtcgtgcca attggttacc ccatcggccg attcggcccg
     1141 gtcaaccgcg cccctgtcga ccacgtcctg cgctggaatc actggtagca gtggccagat
     1201 agggcatgcc gatagacggc acaacgcctg tacggcggcg cataggcagg gtgcactgga
     1261 ctcgtgaagt ctctgctgtt gccccgccgc gatatcgatt tcctgctatt tgaatggctc
     1321 cgcgtcgacg aattgacgaa gcgtgaacgg ttcgccgacc actccaccga gtcgttcggc
     1381 gacgtgctcg ccctttgcga gcaattggca gagcagtgct tcgcgcccca caacaagctc
     1441 gcagacgttc acgaacccgt cttcgacggc accaatgtca ccgtgcttcc agaggtcaag
     1501 gatgctttcg gcgccttctc cgccacgggc ctactcggga tgaccatgga cgaaaagcac
     1561 ggcggagcac agtttcccgt cgttgtcgcc acggcagcgt atctgtggct tcaggccgcc
     1621 aatttcagca ccattagtta ctcactgctg agcttggcca atgcgaattt gcttgcaagt
     1681 tttggaagcg ctgagcaaac cgagaccttc ctgtcgccca tactgtccgg tcggtacacc
     1741 gggaccatgt gcctgtctga gccgcacgct ggctcatcgc tggccgatat caccacccgc
     1801 gcagaacccg aagactccgg cgcctaccgg ctgttcggtt ccaagatgtg gatctccggc
     1861 ggcgatcatg acatcaccga gaacatagtg catctggtgc tggccaagct gcccggcgca
     1921 ccggccggca ccaaaggcat ctcgctattc atcgtgccga agcacctcgt aaacgccgac
     1981 ggctcgattg gcgaacgcaa cgacgtcgtc accgcaggcc tcaaccacaa gatgggccaa
     2041 cacggaatga ccaacgcgct gctgaacttc ggcgatggtg cattccgccc aggcggccgc
     2101 gccggggcag tcggctacct tgtgggtcag ccacaccacg gactcgcata catgttccac
     2161 atgatgaacg aagcacggct gatggcgggt gcggcggcga cagcactggg atacaccgct
     2221 cacctgaagt cgttggagta cgccagaacc cggacacagg gccgtccgat cacatccaag
     2281 gatcccgcga ccccacccgt accaatcatc gagcatcccg acgtcaaacg gatgctctta
     2341 gcccagaagt cctacgtcga gggcgcgctc gcgttggtgc tgtactgttc ccgcctagtc
     2401 gacatcgcgt ccagtgctga gtcacgggag gaagccgagt cggccacact gctgctcgac
     2461 ctgctgaccc ctgtcgccaa aagctggccg tcgcaatggt gtctggaagc caacgacttg
     2521 gcgatccaac tccacggtgg gtacggctac agccgcgaat acgacgtcga gcagcactat
     2581 cgggacaacc ggatcaacgc cattcacgaa ggcacccacg ggatccagag cctcgacttg
     2641 ctgggacgca aggtacgtca gcgcgacggc gccggtcttc ggctgctggc tggcgaaata
     2701 ggccgcacga tcgcggaagc cgatctgctc ggtggggaac ctgaacagct agcgaggcaa
     2761 ctggattggg catggcggcg gctgtccgac accacggcga ctctgttgcg ttgcgat
//
LOCUS       DASRJA010000410         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3841635,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000410 DASRJA010000000
VERSION     DASRJA010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3841635"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VFC01_17855"
     CDS             complement(<1..921)
                     /locus_tag="VFC01_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZN81529.1"
                     /translation="MAPLRTGDGFADVVVTAVASTTSLAPDAEKTWQLLLEGASGVCV
                     LDKPFVGEFKSPVRIGAPLQESFDEHLSRVELRRLAFMQKMSLVLGRRLWENAGTPDV
                     DTRRLMISIGLALGSTEEIPVQYDAWREKGLRAVSPLAVQMYMPNAPAAAVGLDRQAK
                     AGIMSPIMADASGAAAVAEAWRQIVLGEADIAICGGVETHIEAVPVASFWQLGMLSTN
                     NDDPPGACRPFDKDRDGMVFGEGGALLLIETEEHAKARGASILARVMGAAMTSDTYDA
                     VEPDPSGQIAGEAILRAIKLAGLTPTDIDHV"
     gene            1148..>2052
                     /locus_tag="VFC01_17860"
     CDS             1148..>2052
                     /locus_tag="VFC01_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019667793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN81530.1"
                     /translation="MTATMPDTSRLTGQMLIAGSPVRGSGAPIHGFNPSKGQELEPAY
                     PYGDQSHVDAACAAAAAAFSDYRSTTSEQRAQFLETIAANIESIRDDIVARAVAESGL
                     PEARITGEVGRTTGQLRLFADVLREGSWNGARMDPALPDRKPLPRPDIRQRSIPLGPV
                     AVFGASNFPLAFSVAGGDTASALAAGCPVVVKAHDAHPGTSELVGRAITEAVADAGLP
                     AGTFSLLFGSGPELGIALVTDPRIKAVGFTGSRTAGTALVAAAAGRPEPIPVYAEMSS
                     INPVFLLDGALSGRGAELGRAFVASL"
BASE COUNT          328 a          755 c          614 g          355 t
ORIGIN      
        1 gacgtggtcg atgtcggtcg gcgtcagacc ggcgagtttg atggcgcgaa ggatggcctc
       61 gcctgcgatc tgaccgctcg ggtccggctc gacggcgtcg taggtgtcag acgtcatggc
      121 cgcccccatc acccgcgcca ggatcgacgc gccgcgcgcc ttcgcgtgct cctcggtctc
      181 gatgagcagc agcgcaccgc cttcaccgaa caccatgccg tcgcgatcct tgtcgaacgg
      241 gcgacaggcg ccgggcgggt cgtcgttgtt cgtcgacaac atccccaact gccagaagga
      301 ggcgacgggt accgcctcga tgtgcgtttc gacgccgccg cagatcgcga tgtcggcctc
      361 accgagcacg atttgccgcc acgcctccgc gacggccgcg gcgcccgagg cgtcggccat
      421 gatcggcgac atgatccccg ccttggcctg acgatccagc cccaccgccg cggcgggcgc
      481 attgggcatg tacatctgca ctgcgagcgg ggacaccgcg cgcaggccct tctcccgcca
      541 cgcgtcgtac tggacgggga tttcctcggt gctgcccagc gccaggccga tcgagatcat
      601 cagccgcctg gtgtcgacgt cgggtgttcc ggcgttctcc cacagccgcc gccccagcac
      661 cagggacatc ttctgcatga acgcaagcct gcgcagctcg acgcggctca ggtgttcgtc
      721 aaaactttcc tgaagtggcg caccgatccg cactggcgat ttgaactctc cgacaaacgg
      781 cttatccaac acgcagacgc cgctcgcgcc ctccagcagt agctgccacg tcttttccgc
      841 atcgggcgca agcgaagtcg tcgatgcaac cgcggtgaca acgacgtcag cgaatccgtc
      901 gccggttctc aacggtgcca tgctgccatt ccttcacttg ctagctgacc gtgttgaggt
      961 gcctgccacc acagtaagag tctttcggga gtgccgacag cctttcccgt ggcaagagcc
     1021 actaaacaac ggctaagcga ttaatccaac ccctgcatca gtcgcatgta gatcggtctt
     1081 ggacaagcat cgatgcggct cctaccgtta ctgatagacc acgaccaccc gcggttgtta
     1141 ggacaccatg acagcaacga tgcccgacac gtctcgcctg actggacaga tgctcatcgc
     1201 cggctcgccc gttcgcggat ccggcgcccc gatccacggg ttcaatccga gcaaggggca
     1261 ggaactcgaa ccggcctacc catacggcga ccagtcccac gtcgacgccg cgtgcgccgc
     1321 tgccgccgcg gcgttctcgg attaccggtc gacgacgtcc gagcagcgcg cccagttcct
     1381 cgagaccatc gcggccaaca tcgagtccat ccgcgacgac atcgtcgccc gcgccgtcgc
     1441 agaatccgga ctcccagaag cccgcatcac cggtgaggtt ggccgcacga ccgggcagct
     1501 gcgcctgttt gccgacgtgc tccgcgaagg cagctggaat ggcgcccgga tggaccctgc
     1561 gctccccgac cggaagccat tgccacgtcc tgatattcgc cagcggtcca tcccgctcgg
     1621 acccgttgct gtttttggtg ccagcaactt cccgctggcc ttctccgtcg ccggcggcga
     1681 caccgcgtcg gcgctggccg ctggatgccc cgtcgtcgtc aaggcccacg acgcccaccc
     1741 cggcacgtct gaacttgtcg gccgggccat caccgaggcc gtcgccgatg caggcctgcc
     1801 tgccggcacc ttttctctgc tgttcgggtc cgggcccgag ctcggcatcg ccctcgtgac
     1861 cgacccgcgc atcaaggccg tcggcttcac cggatcccgc accgcgggaa cagctctcgt
     1921 cgccgcggca gcgggccgcc ccgagcccat tcccgtctac gccgagatga gctcgatcaa
     1981 ccctgtgttc ctcttggacg gcgcgctgtc tgggcgcggc gctgagcttg gccgcgcatt
     2041 cgtcgcgtcg ct
//
LOCUS       DASRJA010000411         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1776250,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000411 DASRJA010000000
VERSION     DASRJA010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776250"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1434)
                     /locus_tag="VFC01_17865"
     CDS             complement(46..1434)
                     /locus_tag="VFC01_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HZN81531.1"
                     /translation="MSSSALTEPTFTGHRPAAAGDLSVETHGIAPIPKDQRYGSPARL
                     FTVWFAPQVNMTGVFTGALAIVLGLGFWLGLLAMVIGTVLGSLIVGYLSTWGPRTGAG
                     QLPNSRMAFGGGVVLPAVLQWVGTIAWDALVGLFGGQALSLLLGIPFWLAVLIVLGVQ
                     GAAGFFGYEVIHRMQAVLTVVLFVTFVVFAVKLVGGHEIVTPASVNGPDLVGAFVLEV
                     TIAFSLAVSWASYAADFSRYLPADSSRARVFGFTFAGVVLAYIFIQGIGVAAGEVVSD
                     QTAEGVRSVMGGGLLGGLALLIIALASIGSGVMNDYSGSLALQTIGVRVRRPVSAVIV
                     TLLAFPLILWMNSADTATRFQDVLLLVSYWIPAFVSIVVIDWLLRIRGRATIDPAVEP
                     TDRRDAVAAVIVFVLSYAAAIPFMHTSLIEGPIARAWHGADAAYFVNLLVAALLYGGY
                     RLLRSRSSASVG"
     gene            1485..2084
                     /locus_tag="VFC01_17870"
     CDS             1485..2084
                     /locus_tag="VFC01_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN81532.1"
                     /translation="MLRLVLLGVGAMNSPRYRPAGLLLTWPGHRVMLDGGGAADPCLP
                     LDAWLVCDDRAELMSQIRRRARELKVRPAVEPFDADDVQLRPLPVRHTSHPTAGYLIE
                     TPSHRAAWAPEFWEFPDWSADADLMFADAAGWNRPIRFAGGVGGHACVRDVAAAARRA
                     GVRRLVFAHIGRPSIRAIDRGERPPFGQWGYDGAEFRLR"
     gene            2099..>2753
                     /locus_tag="VFC01_17875"
     CDS             2099..>2753
                     /locus_tag="VFC01_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN81533.1"
                     /translation="MPEQRSIWSGGAVGRIGGGAYDFAVEHEWLARPAGLALWGTDTR
                     MLYDSIRTIGELPEGSAVLDVPCGGGVALRGLRPGQSLRYVAADISTDMLDRARARAA
                     ALGRDDIEFVEADIERMPFEDNEFDLCLSFNGLHCLPDPPAAVHEIARCVKPGGRLVG
                     DSVVRGAGWRQDLAIGFFRRAGVFGVGGTVDDLNGWLTDAGLKVDRLERSGGLAHFTA
                     "
BASE COUNT          508 a          936 c          902 g          407 t
ORIGIN      
        1 cagcgaagtg gtggacctga tcgaggattt catcagcacc acgggtcaac caacggaagc
       61 ggacgagcgg gaccgcagca gccgatagcc gccgtaaagc aaggccgcta caagcaaatt
      121 cacgaaatat gcggcgtcgg caccgtgcca cgcccgcgcg atcggaccct cgatcagtga
      181 cgtgtgcatg aacgggatcg ccgcggcgta ggagagcacg aacacgatca ccgcggccac
      241 cgcatcgcgg cgatcggtcg gttccacggc cgggtcgatg gtcgccctgc cgcgaatgcg
      301 gagcagccaa tcgatgacca cgatcgagac gaacgcgggg atccagtaac tcaccaacag
      361 cagcacatcc tggaagcggg ttgcggtgtc ggcggagttc atccacagga tgagcggaaa
      421 ggcaagcagc gtcacgatca cggcagaaac cggccgccgc acccgcaccc cgatcgtctg
      481 cagcgcaagc gagccgctgt agtcgttcat cacgcccgag ccgatcgagg ccagcgcgat
      541 gatcagtagg gccagcccgc ccagcagacc gccgcccatc accgaacgca caccctctgc
      601 cgtctgatcg gagaccacct caccggccgc gactccgatg ccctgaatga agatgtacgc
      661 caacacaact ccggcgaagg tgaacccgaa cacccttgct ctcgacgagt cggcaggcag
      721 gtagcggctg aagtcggcgg catagctggc ccacgacacc gccaggctga acgcgatcgt
      781 cacctcgaga acgaacgcgc cgacgaggtc cgggccattc acactcgccg gcgtgacgat
      841 ctcatgtccc ccaacgagtt tcaccgcgaa caccacgaac gtcacgaaca acaccaccgt
      901 cagcaccgcc tgcatccggt ggatgacctc gtagccgaag aaccccgccg caccctgcac
      961 acccagcacg atcagcactg cgagccagaa cgggatgccg agcagcaggg aaagcgcttg
     1021 cccaccgaac aggccgacca gcgcgtccca tgcgatcgtc cccacccatt gcagaacggc
     1081 aggcaagacg acaccgccgc cgaacgccat ccgggagttg ggcaactgac cggcgcccgt
     1141 gcgtgggccc caggtcgaca gatatccgac gatgagcgag cccagtacag tgccgatcac
     1201 catcgccagc aggccgagcc agaaacccag cccgagcacg atggccagcg cgccggtgaa
     1261 gacgccggtc atgttgacct gcggcgcgaa ccacaccgtg aacagccggg cgggcgagcc
     1321 gtatcgctga tccttcggga tgggcgcgat gccgtgcgtc tcgacggata gatctccggc
     1381 ggcggcgggt cggtgaccgg tgaaggtcgg ctcggtcagt gcactggacg acattcgtcc
     1441 attcgatcac gacgacgctc acacaatgga agtggagtgg tttgatgctg cggctggtcc
     1501 tgctgggcgt cggggcgatg aattcacccc ggtaccgtcc cgccgggctg ctgctgacat
     1561 ggcccggcca ccgggtcatg ctcgacggcg gaggtgccgc ggatccttgc ctgccgcttg
     1621 acgcatggct cgtgtgcgac gaccgtgcgg agctgatgtc gcagatccgg agacgggctc
     1681 gtgaactcaa ggtccggccc gccgtcgagc ccttcgatgc cgacgacgtg cagctgcgac
     1741 cactgccggt acggcatact tcgcacccga ccgccggcta cctgatcgag acgccgtcgc
     1801 atcgtgccgc ctgggcgccg gagttctggg agttcccgga ctggtcggcc gacgcggatc
     1861 tgatgttcgc cgacgcggcg ggatggaacc ggccgatccg gttcgccggc ggcgtcggcg
     1921 gccacgcctg cgtgcgcgac gtcgccgccg cggcccgtcg cgcgggtgta cgacggctgg
     1981 tgttcgccca catcggtcgg cccagcattc gagcgatcga ccgcggcgaa cgcccgccat
     2041 tcggccagtg gggttatgac ggcgccgagt tcaggctgcg ctgactactg tcaacggcat
     2101 gccagagcaa cgatcgatat ggagtggcgg ggcggtcggg cggatcggcg gcggtgccta
     2161 cgactttgcg gtggagcacg aatggcttgc caggcccgcc ggactcgcgc tgtggggcac
     2221 tgatacccgc atgttgtacg acagcatccg cacgatcggc gagctccctg agggttccgc
     2281 ggttctcgac gtcccgtgcg gcggcggggt cgcgctgcgc ggcctgcgac ccggccagag
     2341 cctgcgctac gtcgccgccg acatctccac cgatatgctc gatcgcgcac gcgcccgggc
     2401 agccgcgctc ggccgcgacg atatcgagtt cgttgaggct gacatcgaac ggatgccgtt
     2461 cgaggacaac gaatttgacc tgtgcctgtc cttcaacgga ttgcactgcc ttcccgatcc
     2521 acccgcggcg gtccatgaaa tcgctcgctg cgtcaagccg ggcggtcgtc tggtcggtga
     2581 ctcggtggta cgtggcgcgg gatggcgtca ggatctggcg atcggcttct ttcgccgcgc
     2641 aggcgtcttc ggcgtcggcg gcacggtcga cgacctgaat ggctggctta ccgacgccgg
     2701 cctcaaagtg gaccgtctcg aacgctccgg cggcctagcg cacttcaccg cga
//
LOCUS       DASRJA010000412        15479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1021028,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000412 DASRJA010000000
VERSION     DASRJA010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15479
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021028"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..681
                     /locus_tag="VFC01_17880"
     CDS             172..681
                     /locus_tag="VFC01_17880"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005516433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZN81534.1"
                     /translation="MTTKGKLDARFSQATEPVGWQKVSDALAAAELYWLTTVRKDGRP
                     HVTPLIGAWVDDGFVFCTGPEEQKAQNLSASTAVAVTTGVNTWNDGLDVVVEGNAERV
                     TGLDTLTRLADTIREKYDDEWDFTPYDEGFGHTDDSGDSHIAYVFRVPAAKVLAFAKS
                     PHGQTRFRP"
     gene            773..1846
                     /locus_tag="VFC01_17885"
     CDS             773..1846
                     /locus_tag="VFC01_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HZN81535.1"
                     /translation="MADPAVPVPAKAAGGRDLRTVASCRPEVDVVSVVSPLVWAVTVV
                     VLLAIIVLDLTVIARRQRTVTTKDAVRWVLVYIGLAAAFAAGLFVFLPGEAGQQFVAG
                     YITEYSLSVDNLFVFMIIMARFSVPALAQDKVLYIGIVLSLVFRAAFIFAGAAAIAAA
                     SWVFYILGGFLVYTAIQLALEDPDEEPDFHNNAALRFLHRILPISEDYDGRRLVTRAG
                     SRRLLTPLVIVIAAIGMANVVFALDSIPAIFGLTSDAYIIFTANALALMGLRQLYFLI
                     EGLLEKVVYLSKGLAVILGFIGVKLVIEALHHSHLDEVGTIRLPEIGTVTSLLFIVAT
                     LTITTLLSVLKTNRDARPAAEAE"
     gene            complement(1911..2822)
                     /locus_tag="VFC01_17890"
     CDS             complement(1911..2822)
                     /locus_tag="VFC01_17890"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HZN81536.1"
                     /translation="MSDNPFDPSVWQPIDGFGLTDLTYHRHVVDGKPRPTVRVAFDRP
                     EVRNAFRPHTVDELYRVLDHARMSSDIGVILLTGNGPSPKDGGWAFCSGGDQRIRGRS
                     GYQYASGETAETVDPARAGRLHILEVQRLIRFMPKVVICLVNGWAAGGGHSLHVTCDL
                     TLASREHARFKQTDADVGSFDGGYGSAYLAKQVGQKFAREVFFLGRPYTAEQMHAMGA
                     VNEVVDHADLESVGLEWAAAINGKSPQAIRMLKYAFNLTDDGLVGQQLFAGEATRLAY
                     MTDEAVEGRDAFLQKRDPDWTPFPRYF"
     gene            complement(2819..3271)
                     /locus_tag="VFC01_17895"
     CDS             complement(2819..3271)
                     /locus_tag="VFC01_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN81537.1"
                     /translation="MSNSERIRPPWWLKYVNKAMIGVQHLGISFGPVVLTVPGRKSGK
                     PRSTPVTPMTVDGKEYVVGGLPGSDWSANVRAAGEGTLRRGRRIDRVRLVEMSVDEAR
                     PLLREFPIKVPTGVGFIKGAGLVTGPNPDEFEALAGKCPVFRLDPVNA"
     gene            complement(3286..3732)
                     /locus_tag="VFC01_17900"
     CDS             complement(3286..3732)
                     /locus_tag="VFC01_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN81538.1"
                     /translation="MADNRAPRWLKPANSIYKALLRRGLVIGKERAVVLTVTGRTSGK
                     PRSTPVTPMHIAGNRYVVAVYPGADWVRNVRADEAATVTEGRRSERVRLVELRPELAG
                     PVLRLFPTQLPTGVAFVQRAGLVSDGSPDEFEALAGRLPVFRVDPV"
     gene            complement(3736..4422)
                     /locus_tag="VFC01_17905"
     CDS             complement(3736..4422)
                     /locus_tag="VFC01_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZN81539.1"
                     /translation="MGRVDAVLFDYSGTLFRLEEDDSWFEGITVDERDVDGHVQVELM
                     RRLSAPVGQHVEMTPEAHHAWVNRDLEPHLHREAYLHVLRESGLAHHDAEELYGRVID
                     PSSWTPYPDTAEVLTGLHRQGIKTAVVSNIAFDVRPAFHAIGIADHVDEFVLSYEVGA
                     IKPDAAIFETALTRLGVDAAQAVMVGDSDEADGGARAIGCGFALVDPLPTSQRTDGLR
                     AALNDYGLEV"
     gene            complement(4424..5626)
                     /locus_tag="VFC01_17910"
     CDS             complement(4424..5626)
                     /locus_tag="VFC01_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN81540.1"
                     /translation="MEAAKIVATLTRAVGDVGLAEDLAQEALVDALTQWPSSGVPRNA
                     GAWLTTVAKRKAIDYWRRQENLDAKYAAMAHELETQVDEAWDPDRIDDDVLRLIFISS
                     HPVISREGQIALTLRVVGGLTTEEIARAFLTSTATVAARITRAKKTLAAANVPFEVPD
                     RSEYPQRLSAVLSVIYLVYNEGYSASFGQRWIRDELCSEALRLSRVLAALVPDEAEVH
                     GLVSLMEFQSSRFASRTDSDGRPILLEDQNRAKWDRAQIQRGVAALERAANTLQRKGA
                     GWGPYALQAALAECHAVAPTAADTDWARIVLLYDGLRQVAPSPVVELNRAVAVAMADG
                     PADALEIVDKIDGLDGSYLLPSVRGELLARLGRDAEAADEFDRAAKMTDNEREREVLA
                     GKAARMRG"
     gene            complement(5687..6346)
                     /locus_tag="VFC01_17915"
     CDS             complement(5687..6346)
                     /locus_tag="VFC01_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN81541.1"
                     /translation="MPAHLEITDDIAILNLGDDENRFSPDWLATVNGYLDRIESGEAK
                     ALVTTGDGKFYSNGLDLDWLAAHSDQGDWYVDQVQHLLARVLVAPVPTVAAVNGHAFG
                     AGAMLGMAHDFRVMRVDRGYYCFPEVDIHIPFTPGMAALIQAKLTPSAAIEAMTTGRR
                     YGGADAEAIGLVTATAPEDKLASTAIDLVAPLKGKDPGTLRAIKSTMFAGVTAALRGT
                     A"
     gene            complement(6318..6938)
                     /locus_tag="VFC01_17920"
     CDS             complement(6318..6938)
                     /locus_tag="VFC01_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN81542.1"
                     /translation="MPRPQVYDRDAMLDAAEKLAVEGGVKAVTIRAVSERAGISNGAI
                     YHAFGSRGGLAGRAWVRAARRFLALQRQSVDAEAEPVRAVIAAADAPAVFSEQYPSSA
                     RLILGISREELIGDAPADVQAELRALDRDLRDLLIRLSIAVFDRKDAASVAAIEDCVV
                     GLPTGLMLRRPMPPTADTRRRLAAAVEAVLSVKLGDSNARTSRNHR"
     gene            7040..7249
                     /locus_tag="VFC01_17925"
     CDS             7040..7249
                     /locus_tag="VFC01_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81543.1"
                     /translation="MAHRLVVAYREGRKAFPNTLLNPYAGVGDRAVARMWRLGWQRAA
                     EESRGIPSEAERIERFRAEIDALLD"
     gene            complement(7318..8919)
                     /gene="fadD8"
                     /locus_tag="VFC01_17930"
     CDS             complement(7318..8919)
                     /gene="fadD8"
                     /locus_tag="VFC01_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase FadD8"
                     /protein_id="HZN81544.1"
                     /translation="MTDELLRHPLHSGHLTVGALKRNKDKPVLFLGDTTLTGGQLADR
                     ISQYIQAFEALGAGTGAAVGLLSLNRPEVLMIIGAGQTQGYRRTALHPLGSLDDHAYV
                     LSDAEVTSLIIDPNPMFVERAQGLLDKVPSLKQVLTIGPVPEALAGVAVDLNAEAAKY
                     SPRPLAAADLAPDHIGGLTYTGGTTGKPKGVIGTTQSITTMTTIQLAEWEWPEHPKFL
                     MCTPLSHAGAAFFTPTIVKGGELVVLTKFDPAEVLRVIEEQKITATMLVPSMIYALMD
                     HPDSHTRDLSSLETVYYGASAMNPVRLKEAISRFGPIFAQYYGQSEAPMVITYLAKGD
                     HDEKRLTSCGRPTLFCRVALLGEDGKPVPQGEVGEICVSGPLLSGGYWNLPEATAETF
                     RDGWMHTGDLAREDEDGFYYIVDRTKDMIVTGGFNVFPREVEDVVAEHPSVAQVCVIG
                     TPDEKWGEAVTAVVVLRPDAPSDEEAVATMTAEIQAAVKERKGSVQSPKQVIVVDSVP
                     VTALGKPDKKSVRAQFWQGASRAVG"
     gene            complement(9008..9466)
                     /locus_tag="VFC01_17935"
     CDS             complement(9008..9466)
                     /locus_tag="VFC01_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZN81545.1"
                     /translation="MSRYMLIMRSTPEAEKAMAEQNIDFNEIIEAMGRYNEELIKAGV
                     MLAGEGLTGPDEGFVVDFNSDPPVVTDGPYTEAKELFNGFWILDVSSKEEAKQWAQKC
                     PLGTGVKLEVRRVSEAEEFPQDNPWIEKEIQWRADLAQKVADQAAKAAGR"
     gene            9602..11208
                     /locus_tag="VFC01_17940"
                     /pseudo
     CDS             9602..11208
                     /locus_tag="VFC01_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
     gene            11239..12807
                     /locus_tag="VFC01_17945"
     CDS             11239..12807
                     /locus_tag="VFC01_17945"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HZN81546.1"
                     /translation="MSELPDPRFLDERTAHWAEAKPDGEAFTYFDRTWTWSEWNDRVR
                     RLAGALKDRGVKRGDVVAFLDKNHPACVELTIAAASLGAANAIINFRLAADELDYVLN
                     DSDAKVLIVGAELKPGVDKIRDKLANVEHIITVTPEGGDGDEYEAMLADATPTGRADD
                     VEPDDVCILMYSSGTTGRPKGVELTQANIIAHTVNAHEGFEFDEGDKNMVSMPLFHVG
                     GSSYVQFGIHDGVPSVMTRDVDGMTLAGAILKGANRTFLVPAVLAKVLESGEDAVKLF
                     GALKTYAYGASPMPLPLLRMALEAWPNTDFIQAYGLTELCGVISHLLPEAHRDPGREE
                     RLSSAGTLVPNAEVRVVDPDTLKDVPEGEQGELWFRSPQLMKGYHNKPEATKESITED
                     GWFRTGDIGRVDDGGYIFVEDRLKDMIISGGENIYSIEVERVLAEHPAVVDVAIIGVP
                     DEKWGEVVKAVVQLEGEADEKELIAFCRERLAAYKCPKTVDIKDELPRNPTGKILKKD
                     LRKQYWEGRDRATV"
     gene            12817..13779
                     /locus_tag="VFC01_17950"
     CDS             12817..13779
                     /locus_tag="VFC01_17950"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HZN81547.1"
                     /translation="MAPPLDDLLDRLHVVSLPMRVRFRGIMVREVALIDGPAGWGEFG
                     AFLEYEPPEATAWLASAIDAAYGEPQPTHRDRIPINATVPAIPALEVPKVLARFPGVR
                     TAKVKVAEPGQSLVDDVDRVEVVRALVPTVRVDANGGWTVDEAVAAATALSATGPLEY
                     IEQPCTTIEELAEVRRRVDVPIAADESIRKAADPLRVVRAGAADIAVLKVAPLGGVPK
                     LLEIAAQIDVPVVISSALDSAVGIATGLRAAAALPSLEHACGLGTGALFMDDVVEPLT
                     PVDGCLPVAPITPDPARLETLAASPERRQWWIDRVSACHRKLVE"
     gene            13857..15002
                     /locus_tag="VFC01_17955"
     CDS             13857..15002
                     /locus_tag="VFC01_17955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN81548.1"
                     /translation="MAGMRVGLMDMIIAGRPAVDTFTRANYLGAVAAGVDSFWVPDHL
                     NALFPRALWQPKYCGGAKILPSADAYLEPWTMLGHLAARNRIGRLRLGVGVTDTGRRN
                     PAVTAQAAATVQLLTRGRAILGIGTGEREGNEPYGVDWSKPVARFEEAMATIRALWDS
                     KGELVNRDSPYFPLRNALFDLPPYKGRWPEIWIAAHGPRMLRTVGRYADGFLPAFAHK
                     PQEYTRRLEVVRAAASDAGRDPMAIVPAMWLPVLTGRTAGEIEAALESEVIRAGALNA
                     TDEFFAEHGLQHPLGVGFKGGQDLMPQDWDEQTALANIKGIPPSFLREMALVGTPSEV
                     IDRAAEWRDCGVRYLVVVNGAFMQRSLRNAIGATLPFMKVVRGLKKL"
     gene            15081..>15479
                     /locus_tag="VFC01_17960"
     CDS             15081..>15479
                     /locus_tag="VFC01_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZN81549.1"
                     /translation="MVVLGYPGVQALDLVGPFEVFTGATIYLASQGRADEGYAVSVVS
                     RNGEPATTLTGLALVAQPLPDPREPIDTLVLPGGIGVDDARRNADTVGWIQIATEHAR
                     RVVSVCTGSFLAAQAGLIDGCVATTHWAFAG"
BASE COUNT         2586 a         5183 c         5077 g         2633 t
ORIGIN      
        1 aaggcgatgg gatcacggcg gcgccgcagc gcctgttcgg tcagtgctgg tcgcatgctg
       61 gtcagcagca gcctttcgga cagccggcgc agcggactct tgctcacgga tcatgagttt
      121 aggtggccta tttttcgatg aattgtcggc cgcggcgcgg tcggtatctg tatgacgact
      181 aaaggcaagc tcgacgctcg tttcagccag gcaacggagc ccgtcggctg gcagaaggtc
      241 agtgacgccc tggccgccgc ggagctgtac tggctcacca ccgtccgcaa ggacggccgt
      301 ccgcatgtca ctccgctgat cggcgcatgg gtcgacgacg gcttcgtgtt ctgcacggga
      361 cccgaggaac agaaggcgca gaacctctcc gcctccaccg cagtggcggt gaccaccggc
      421 gtcaacacgt ggaacgacgg actcgacgtt gtcgtcgaag gcaacgccga gcgagtgact
      481 gggctcgaca cgttgacccg gctggccgac acgatccgcg aaaagtacga cgacgaatgg
      541 gatttcacgc cttacgacga gggtttcggc cacaccgacg acagcggcga cagccatatc
      601 gcctatgtct tccgggtgcc tgcggcgaag gtgctggcgt tcgcgaagtc accgcacggc
      661 cagacccgct tccgcccgtg agtcgacacg ttctgcatag cggactatag ttctgctcgt
      721 tcgtggaggg gagtattcct cgcgcggcac cgtcgtcatc acggacggcc ccatggccga
      781 cccggcggtg ccggtcccgg ccaaggccgc gggcggaaga gacctccgga ctgttgcttc
      841 gtgccgtccg gaggtcgacg tggtttctgt tgtctcgccg ctggtttggg ctgtcacggt
      901 tgtagttctg ctcgcgatca tcgtgctcga cctgacggtg atcgcccggc gacaacgaac
      961 cgtcaccacc aaagatgccg tccgctgggt gctcgtctac atcggcctcg ctgcggcctt
     1021 cgcggccggg ctgttcgtct tcctgcccgg cgaggcgggg cagcagttcg tcgccgggta
     1081 catcaccgag tacagcctga gcgtggacaa cctcttcgtg ttcatgatca tcatggcccg
     1141 gttctcggtg cctgcactgg ctcaggacaa ggttctgtac atcgggatcg tgctgtcgct
     1201 ggtgtttcgt gccgcgttca tcttcgccgg tgcggcggcg atcgccgcgg cgagctgggt
     1261 gttctacatc ctcggcggat tcctggtcta caccgcgatc cagttggcgc ttgaagatcc
     1321 cgacgaggag ccggatttcc acaacaacgc cgcgctgcgg ttcctgcacc ggatcctgcc
     1381 gatcagtgag gactacgacg ggcggcgtct ggtcacacgc gcgggcagcc gccgcctgct
     1441 caccccgctg gtgatcgtca tcgccgcgat cggcatggcc aacgtcgtgt tcgcgctcga
     1501 ctcgatcccg gcgatcttcg gcctgacgag cgatgcgtac atcatcttca cggccaacgc
     1561 actcgcgctg atgggcttgc ggcagctgta cttcctcatc gaaggcctgc tggaaaaggt
     1621 cgtctatctc agcaagggcc tcgccgtcat cctcggtttc atcggcgtga agctcgtcat
     1681 cgaagcgctg caccactccc acctcgacga ggtcggcacg atccgcctgc cggagatcgg
     1741 gactgtgacg tcgctgctgt tcatcgtcgc caccctgacc atcacgacat tgctcagcgt
     1801 gctcaagacc aaccgcgacg cccgccccgc cgccgaggcg gagtgatttc ggtgcgcaaa
     1861 cggtcgctga gcgacctgtt cgctgagcga ctgttagcac accgaaatca ctagaagtag
     1921 cgggggaatg gtgtccagtc cgggtcgcgc ttctgcaaaa acgcgtcgcg accctcgacg
     1981 gcctcgtcgg tcatgtacgc caatcgcgtt gcctcaccgg cgaatagctg ttggccgacc
     2041 agcccgtcat cggtcagatt gaacgcgtac ttgagcatcc ggatggcctg cggtgacttg
     2101 ccattgatcg ccgccgccca ctccagccca acggattcca ggtccgcgtg gtcgacgacc
     2161 tcattgaccg cacccatcgc atgcatctgc tcggccgtgt acggccggcc gaggaagaag
     2221 acctcgcgag cgaacttctg gcccacctgt ttcgccaaat acgcactgcc ataaccgccg
     2281 tcgaagctgc cgacgtcggc gtcggtctgc ttgaaccggg cgtgctcgcg gctggccagc
     2341 gtcaggtcac atgtcacgtg caggctgtgc ccgccgccgg ccgcccaccc gttgaccagg
     2401 cagatgacca ccttcggcat gaaccggatc agccgctgca cctcgagaat atgcagtcgc
     2461 cccgcacgcg ccgggtccac cgtctccgcc gtctcgcccg atgcatactg atagccgctg
     2521 cggccgcgga tgcgctggtc gccgccggag cagaacgccc agccgccgtc cttcgggctg
     2581 ggcccgttgc cggtcaacag gatgacgcca atgtccgacg acatgcgggc atggtccagc
     2641 acgcggtaca gctcgtcgac ggtgtgtggt cgaaacgcgt tgcgcacctc tggccggtcg
     2701 aacgcgacgc gcaccgtcgg ccggggcttc ccgtcgacga cgtggcgatg gtacgtgagg
     2761 tcggtcaggc cgaacccgtc gatgggctgc cacactgacg ggtcaaaagg gttgtcgctc
     2821 atgcgtttac cgggtccaga cgaaacaccg ggcacttgcc ggcgagggcc tcgaactcgt
     2881 cgggattggg tccggtgacc aggccggcgc ctttgatgaa gccgacgccg gtcggcacct
     2941 tgatcgggaa ctcccgtagc agcggacggg cctcgtcgac ggacatctca accaggcgaa
     3001 cgcgatcgat gcggcggccg cgtcgcagcg tgccttcgcc cgcggcccgg acattggccg
     3061 accaatccga gccgggaagg ccgccgacga cgtattcctt gccatcgacg gtcatcggcg
     3121 tgacgggcgt cgaccgcggc ttgccgctct tgcggccggg cacggtgagc accaccgggc
     3181 cgaagctgat gccgaggtgc tgcaccccga tcatcgcctt gttgacgtac ttcaaccacc
     3241 agggcggtcg gatgcgctca ctgttggaca tgtccgtaac gctactcaaa ccgggtcgac
     3301 gcggaatacg ggtaggcggc ccgccagcgc ctcgaactcg tccggcgagc cgtcgctgac
     3361 caggccggct ctttgcacga acgccacgcc ggtcggcagc tgcgtcggga acaaccgcaa
     3421 caccggcccg gccagctcgg gccgcagctc gaccagccgc acgcgttcgc tgcggcggcc
     3481 ctcggtcacg gtcgcagctt cgtcggcccg aacgtttcgc acccagtccg cgccggggta
     3541 cacggcgacg acgtaccggt tacctgcgat atgcattggc gtgacgggcg tcgagcgcgg
     3601 cttacctgag gtgcgtcccg tcaccgtcag cacgaccgca cgttccttcc cgatgaccag
     3661 gccgcggcgc agcagggcct tgtagatgct gttggccggc ttgagccacc gtggcgcccg
     3721 gttgtcggcc attggctaga cctccagtcc gtaatcgttg agtgcggcgc gcaatccgtc
     3781 ggtgcgctgt gacgtcggca gcgggtcgac gagggcgaaa ccgcagccga tcgcgcgtgc
     3841 accgccgtcg gcctcgtcgc tgtcgccgac catcacggct tgggcggcgt cgacgcccag
     3901 gcgcgtcagc gcggtttcaa atatcgcggc atccggtttg atggcgccga cttcgtagga
     3961 cagcacgaat tcgtcgacgt ggtcggcgat gccgatcgcg tggaaagcgg gacgaacgtc
     4021 gaacgcgata ttggacacga ccgcggtctt gatgccctgt cggtgcaggc ccgtcagcac
     4081 ttcggcggtg tcggggtacg gcgtccagga cgacggatcg atgactcggc cgtacagctc
     4141 ctcggcgtcg tgatgcgcca gcccggattc gcgcagcaca tgcaggtagg cctcgcggtg
     4201 caggtggggt tcgaggtcgc ggttgaccca ggcgtggtgg gcctccggcg tcatctcgac
     4261 gtgctggccg accggggcgc tgaggcgtcg catcaactcg acctgcacgt gcccgtcgac
     4321 gtcacgctcg tcgacggtga tgccctcgaa ccagctgtcg tcctcttcga gccgaaacag
     4381 cgtgccggag tagtcgaaca acacagcatc cactcggccc atcctaacca cgcatccgag
     4441 cggccttgcc tgccaacact tctcgctcac gttcgttgtc ggtcatcttt gctgcgcggt
     4501 cgaactcatc ggcagcctcg gcgtcgcggc ccagccgggc cagtagttcg ccgcgcacgc
     4561 tgggcagcag atacgaaccg tcgagaccgt cgattttgtc gacgatttcc agcgcatccg
     4621 ctggcccgtc agccatcgcc accgcgaccg cgcggttcag ctcgaccacc ggcgacggcg
     4681 ccacctgacg cagtccgtcg tacagcagca cgatgcgcgc ccagtcggtg tccgcggccg
     4741 tcggcgcgac ggcgtggcat tcggccagcg cggcctgcaa cgcgtaagga ccccatccgg
     4801 cgcccttgcg ctgcaacgta tttgccgccc gctcgagggc agcgacgccg cgttggatct
     4861 gcgcgcgatc ccacttggcg cggttctggt cctcgagcag gatgggacgc ccgtcgctgt
     4921 cggtgcgtga cgcgaagcgc gacgactgaa actccatcag cgacaccaaa ccatgtacct
     4981 cggcctcgtc gggcaccagc gccgccagca ccctgctgag ccgcagcgcc tcgctgcaca
     5041 gctcatcgcg gatccaacgc tgcccgaacg acgccgagta accctcgttg tagaccaggt
     5101 agatcacgct cagcacggcc gagagccgct gcgggtattc cgaccggtcc ggcacctcga
     5161 acggcacgtt cgcggcggcc agtgtcttct tggcccgggt gatgcgcgcc gcgaccgtcg
     5221 cagtcgaggt caagaacgcg cgggcgatct cctcggtggt caacccgccg accacgcgca
     5281 gtgtcagcgc gatctgcccc tcccgggata tcaccgggtg cgacgagatg aagatcagcc
     5341 gcagcacgtc gtcgtcgatg cggtcggggt cccacgcctc gtcgacctgc gtctcgagct
     5401 cgtgtgccat cgccgcgtac ttggcgtcga ggttctcctg cctgcgccag tagtcgatgg
     5461 ccttgcgttt ggcgacggtg gtcaaccacg cgcctgcgtt gcgcggcacc ccggacgacg
     5521 gccactgcgt cagcgcgtcg acgagggctt cctgggccag gtcctcggca aggccgacgt
     5581 cgccgaccgc gcgggtcagc gtcgcgacga tcttggcggc ctccatccgc cagaccgcgt
     5641 cgacggtctg ctgtagatcc ggcacgtgcc cattgtgccg agcggctcag gccgtcccgc
     5701 gcagagcggc ggtcacgccc gcgaacatcg tggacttgat cgcccgcagc gtgccgggat
     5761 ccttgccctt caacggcgcc accaggtcga tggccgtcga cgccagcttg tcctcaggag
     5821 cggtcgccgt caccaggccg atcgcctcgg cgtcggcgcc gccgtagcgg cgaccggtgg
     5881 tcatcgcctc tatggccgcc gacggcgtga gcttcgcttg gatcagggcc gccatgccgg
     5941 gcgtaaacgg gatgtggatg tccacctcgg ggaagcagta atagccgcgg tcgacccgca
     6001 tgacgcgaaa gtcgtgggcc attcccagca ttgcgcccgc gccgaaagcg tggccgttga
     6061 cggcggcgac ggtgggaacc ggtgcgacca gcacgcgtgc gagcaaatgc tgcacttgat
     6121 cgacgtacca gtcgccttga tcgctatggg cggcaagcca atccagatcc aggccgttcg
     6181 agtagaactt gccgtcacct gtggtcacca gcgccttggc ctcgccggac tcgatgcggt
     6241 cgaggtagcc gttgacggta gccaaccagt ctggcgaaaa ccggttttcg tcgtcgccga
     6301 ggttcaaaat cgctatgtca tcggtgattt cgagatgtgc gggcattgct gtctcctaac
     6361 ttgacggaca ggaccgcttc caccgcggcg gccaggcggc ggcgcgtgtc ggcagtgggt
     6421 ggcatgggtc tgcgcagcat cagcccggtg gggaggccga cgacgcagtc ctcgatcgcg
     6481 gcgaccgatg cggcgtcctt gcggtcaaac accgcgatcg acaggcggat caacaggtcg
     6541 cgaaggtcgc gatcgagcgc gcggagttcg gcctgtacgt ccgcgggtgc atcgccgatc
     6601 agttcctcgc gcgaaattcc cagtatcagc cgggccgatg acgggtactg ctcgctgaac
     6661 acggcgggcg cgtcggccgc tgcgatgacg gcgcggacgg gttcggcttc ggcgtcgacg
     6721 gactggcgtt gcaatgccag gaagcgtcgg gcggcgcgca cccaggcgcg gccggcgagt
     6781 ccgccgcggg agccgaacgc gtgatagatc gcgccgttgg agatgccggc ccgctcggac
     6841 accgcgcgga tggtgacggc cttgacgccc ccttcgacgg cgagtttttc ggccgcgtcg
     6901 agcatcgcgt cgcggtcgta gacctgtggc cggggcatgg cggccaaggt aacagagcag
     6961 atgctctgtt atcaaatggg tgcgtgacac gccgaagaaa tatgggctgt agcaaccatc
     7021 ggaggcggaa agtagtgcta tggcacaccg gctcgtcgtc gcgtaccgcg aagggcgaaa
     7081 ggccttcccg aatacgcttc tcaatcccta cgcgggggtg ggtgaccgtg ccgtcgctcg
     7141 catgtggcgg ctgggttggc aacgggccgc cgaggagagc cgcggcatac cgtcggaggc
     7201 cgaacgcatc gagcgatttc gcgccgagat cgacgcgctg ctggattagc gcgagcagac
     7261 acaacatgcc caatttgggc cgttacagag cagttttgcg tctgctgccg aagaaaccta
     7321 gcccaccgcg cgcgaggcgc cctgccagaa ctgcgcgcgc accgacttct tgtccggctt
     7381 gcccagcgcg gtcaccggca ccgagtccac gacgatcacc tgcttcggcg actgcaccga
     7441 acccttgcgc tccttgaccg cggcctgaat ctcggcggtc atcgtcgcca ccgcttcctc
     7501 gtcagacgga gcgtcgggcc gcagcaccac cactgcggtc acagcctcgc cccacttctc
     7561 gtcgggggtg ccgatcacgc atacctgagc gaccgaagga tgttctgcca caacgtcttc
     7621 cacctcacgc gggaacacgt tgaagccgcc ggtgacgatc atgtccttgg tccggtcgac
     7681 gatgtagtag aacccgtcct cgtcctcgcg ggccagatcg ccggtgtgca tccagccgtc
     7741 gcggaacgtc tccgccgtcg cctccggcag attccaatag ccgccggaca gcaacggccc
     7801 ggagacgcag atctcgccga cctcgccctg gggcaccggc tttccgtctt ccccgagcag
     7861 cgccacgcga cagaacaacg tcggccgccc gcaggatgtc aaccgcttct cgtcgtgatc
     7921 gcccttggcg agatacgtaa tcaccatcgg tgcctcggat tgcccgtagt actgcgcgaa
     7981 gatcgggccg aaccggctga tggcctcctt gagccgcacc gggttcatcg cagaggcgcc
     8041 gtagtacacc gtctccagcg atgacaggtc gcgcgtgtgc gaatccggat ggtccatcag
     8101 cgcgtagatc atcgacggca ccagcatcgt cgcggtgatc ttctgctcct cgatcacgcg
     8161 cagcacctcg gcgggatcga acttggtcag cacgactagt tcgccaccct tgacgatcgt
     8221 cggcgtgaag aacgccgcgc cggcgtgcga cagcggtgtg cacatcagga acttcggatg
     8281 ctcgggccac tcccattcgg cgagctggat agtcgtcatc gtggtgatgg attgcgtcgt
     8341 gccgatcacg cccttgggct tgccggtggt gccgccggtg taggtcagcc cgccgatgtg
     8401 atcgggcgcc aggtcggcgg ccgccaatgg ccgcggcgag tacttcgccg cctccgcgtt
     8461 caagtcgacc gccacgcccg ccaaggcctc gggaaccggg ccgatggtca gcacctgctt
     8521 gagcgacggc accttgtcca gaagcccctg cgcccgttcg acgaacatcg ggttcgggtc
     8581 gatgatcagc gacgtgacct cggcgtcgga caacacatat gcgtggtcgt ccaacgaacc
     8641 gagagggtgt agtgccgtgc gccggtatcc ctgcgtctgc ccggcgccga tgatcatcag
     8701 cacctcgggc cggttcagcg acagcagtcc gacggcggcg ccggtgcccg cgcccagcgc
     8761 ctcgaacgcc tgaatgtact ggctgatgcg gtcggccagc tggccgccgg tcagcgtggt
     8821 atccccgagg aacagcacgg gcttgtcctt gttgcgcttg agagcgccga ctgtgaggtg
     8881 gccggagtgg aggggatggc gcagcagctc gtccgtcatg acgtcaagac tagaacgtgt
     8941 tacagaacga gctactgctt ccccgtgcat ccggctaaag cggatgccca gggacccccc
     9001 gtgaaaatca gcggcccgcg gccttggcag cctggtcggc caccttctga gccaggtcgg
     9061 cccgccactg gatttccttc tcgatccacg ggttgtcctg cgggaactcc tcggcttcgg
     9121 aaacccggcg cacctcgagc ttgactccgg tgccaagcgg gcatttctgc gcccactgct
     9181 tggcctcttc cttcgacgac acgtcgagga tccaaaaccc gttgaacagc tccttggcct
     9241 cggtatacgg gccatcggtg acgaccggcg gatcggagtt gaagtcgact acgaagccct
     9301 cgtcgggtcc ggtcaggccc tcgccggcga gcatcacacc cgccttgatg agctcctcgt
     9361 tgtagcggcc catcgcctcg atgatctcgt tgaagtcgat gttctgttcc gccatggcct
     9421 tttcggcctc gggggtcgac cgcatgatca gcatgtagcg cgacattgtg gtgtctcctt
     9481 ctagttggga aggggcgcgt ccatcgtgcc ctcgttgaca cgtcgaacgg caacccgccg
     9541 aaatcgacac ggccctcgaa aatttctcaa agaatctttc cagcgctgaa atgatggccc
     9601 aatgccccct gctgacctgg tcattcgtgg aaccgtgctc accgtcgacg ttctgcagcc
     9661 gaccgcggag gcactggcgg tgtccgatgg ccgcatcatc gcggtcgggg accggtcagc
     9721 cgtcgagaac tggatcgggc ccaacacccg gacgctcgac atcgacggct gcgtgatgcc
     9781 tggcttcgtc gaggcgcacg gccatccgct gatggaggcc atcgtgctct ccgaccggat
     9841 ggtcgacatc cggcccgtca ccatccgcag cgccgacgac gtcctcgccg cgatccgcgc
     9901 cgaagtcgcc aagcgagggg ccgacggcgc ctacctcaac ggctgggatc ccttgctgca
     9961 gaacggactt cctgggctga cgcttgactg gctgaaccgg gaagcccctg acgcccgctg
    10021 gtgatcgtcc acaactcagg ccacaaagca tggttcaaca ccgccgcggc caatggggcc
    10081 ggattgactc gcgacacccc ggacccgaag ggcgccaagt tcggccgcga cgccaacggc
    10141 gatctcgacg gcaccgccga agaaaccgcc gccttgttcg ccgtgatgtt cggcgccgtt
    10201 cagcccgaca actatccggc ggaacttcgc gacgaatgcg cccggctgaa ccgggccgga
    10261 ctgacgacgt gctcggagat ggcgttcgat ccgtcgctgc ggccgatcat cgagcagatg
    10321 cgcgacgaac tcaccgtccg cttccgcgtg tatgagatct gcaccgccga gatgcacacc
    10381 gatatgaccc ccgccaacgg cgatgacatg ttccgccagg tcggcatcaa gatctgggtc
    10441 gacggctcgc cgtggatcgg caacattgac ttgagcttcc cgtacatgga caccgacgcc
    10501 acccgcacca tcggtgtcga accgggatcg tgcggtcacg cgaactacac ccgcgagcag
    10561 ctgacggaga tcgtcggcgc gtacttcccg aagggctggc cgatggcctg ccatgtccag
    10621 ggcgacgccg gcgtcgacac gatcctcgac gtctacgaag acgcgctgcg gcgctggtcc
    10681 cgcgacgacc accggctgcg gctcgagcac gtcggcgcca tccggcccga gcagctgcag
    10741 cgcgcccacg acctcggcgt cacgtgcagc ctgttcgtcg accagatcca ctattggggc
    10801 gacgtcatcg tcgacagcct gttcggcccc gagcacggcg agcggtggat gccgtgcggc
    10861 accgcagtca agacgggcat gaggatctcg ctgcacaacg acccgcccgt cacgccagaa
    10921 gaaccgctgc gcaacatcag cgtcgcggtc acccggaccg cgccctccgg ccgtgtgttg
    10981 gcgccggaag agcggctgac cgtcgagcag gccatccggg cgcagacgat cgacgcggcg
    11041 tggcagctgt tcgccgatga cgtgatcggc tcgctggagg tcggcaagta cgccgacctg
    11101 gtcgtgctgt cggccggtcc gcgcgccgtg ccgcccaagg agatcgccga tctcgaggtg
    11161 cgggcgacgt tcgtggcggg tcggcaggtt tacccgaaag aggtctgaca cacgggcgac
    11221 cgtggcacgc ttctgatcat gtctgagctg cccgacccgc gctttctcga cgaacgcacc
    11281 gctcactggg ccgaagccaa gcccgatggg gaggcgttca cctatttcga ccggacctgg
    11341 acatggtcag agtggaacga ccgtgtgcgc cggctggccg gtgcgctgaa agatcgcggc
    11401 gtcaaacgcg gcgacgtcgt ggcgttcctg gacaagaacc acccggcctg cgtcgagctg
    11461 acgatcgccg ccgcgtcgtt gggcgccgcg aacgcgatca tcaacttccg gctcgccgca
    11521 gacgaactcg actacgtgct caacgactcg gatgccaagg tcctgatcgt cggcgccgaa
    11581 ctcaagccgg gcgtcgacaa gatccgcgac aagctcgcca acgtcgaaca catcatcacg
    11641 gtgacgcccg agggtggtga cggcgacgag tacgaggcga tgctggccga cgccacgcct
    11701 actggccgcg ccgacgacgt cgaacccgac gacgtgtgca tcctcatgta ttcgtcgggc
    11761 accaccggcc gcccgaaagg tgttgagctg acgcaggcca acatcatcgc gcacaccgtc
    11821 aacgcgcatg aggggttcga gttcgacgag ggcgacaaga acatggtgtc gatgccgctg
    11881 ttccacgtcg gcggctcgtc ctatgtgcag ttcggcattc acgacggcgt cccgagcgtg
    11941 atgacccgcg atgtcgacgg catgaccttg gccggcgcaa ttctcaaggg cgccaacaga
    12001 acattcctgg tgcccgcggt gctggccaag gtgctggaat cgggcgagga cgccgtcaag
    12061 ctgttcggcg cgctgaagac ctacgcctac ggcgcgtcgc cgatgccgct gccactgctg
    12121 cgcatggcac tggaggcatg gccgaacacc gacttcatcc aggcatacgg gctgaccgaa
    12181 ttgtgcggcg tgatcagcca tctgcttccg gaggcccacc gcgaccccgg cagggaggag
    12241 cggctgtcca gtgccggcac gctggtgccc aacgcggagg tgcgtgtcgt cgacccggac
    12301 acgctcaagg atgtgccaga gggcgagcag ggtgaattgt ggttccgctc accacaattg
    12361 atgaagggtt accacaacaa gccggaggcg acgaaagagt cgatcactga ggacgggtgg
    12421 ttccgcaccg gcgacatcgg ccgcgtcgac gacggcggtt acatcttcgt cgaggaccgg
    12481 ctgaaggaca tgatcatctc tggcggagag aacatctact cgatcgaggt ggagcgagtg
    12541 ctggccgaac atcccgcggt ggtcgacgtc gcgatcatcg gggtgcccga cgagaagtgg
    12601 ggcgaagtcg tgaaagccgt tgtccaactt gaaggcgagg ccgacgagaa agagttgatc
    12661 gcgttctgtc gcgagcggct ggccgcctac aagtgcccaa aaaccgtcga tatcaaggac
    12721 gagctgccgc gcaacccgac cggcaagatc ctgaagaagg atctgcgcaa gcagtactgg
    12781 gagggccgcg accgagccac ggtctagcct ggtcgcgtgg ccccaccgct cgacgacctg
    12841 ctcgaccgcc tgcatgtcgt ctcgctgccg atgcgagttc gctttcgcgg catcatggtt
    12901 cgcgaggtcg cactgatcga cgggccggcc ggatggggcg agttcggggc attcctggag
    12961 tacgagccgc cggaagccac cgcgtggctg gcgtcggcga tcgacgcggc atatggcgag
    13021 ccgcagccaa cgcaccgcga tcgcatcccg atcaacgcga cggttccggc gataccggcg
    13081 ctggaggtgc ccaaggtgct ggcccggttt ccgggtgtgc gtacggcgaa ggtcaaggtc
    13141 gccgagccgg gacagagtct ggtcgacgac gtcgaccgtg ttgaggtggt tcgcgcgctt
    13201 gttccgacgg tgcgtgtcga cgccaacggc ggatggaccg tcgacgaagc cgtggccgcc
    13261 gcaacggcgt tgagcgccac cgggccactg gagtacatcg agcagccgtg cacgacgatc
    13321 gaggaactgg cggaggtccg gcgccgggtc gacgtgccga tcgccgccga cgagagcatc
    13381 cgcaaggccg ccgaccccct gcgggtggtg cgcgccggcg ccgccgatat cgcggtgctg
    13441 aaggtcgcgc cgctgggcgg cgtacccaag ctgctggaga tcgcggccca gatcgacgtt
    13501 ccggttgtca tctccagcgc gctggactcg gccgtcggta tcgcaaccgg gctgcgcgcc
    13561 gcagctgctc tgccgagcct cgaacacgcc tgcgggctcg gcacgggtgc gctctttatg
    13621 gatgacgtcg tcgagccgtt gacgccggtg gacgggtgcc ttcccgtcgc gcctatcact
    13681 ccggacccgg cccggctcga aacgctcgcc gcctcgcccg agcgtcggca atggtggatc
    13741 gaccgcgtca gtgcttgcca tcgaaagtta gtcgagtaga ggcgtactct atgccccccg
    13801 attgagcggc gagacactga aactcagcaa catcgtcgaa ccggggggag tcaactgtgg
    13861 ctggaatgcg cgtgggcctg atggacatga tcatcgccgg caggccagcc gtcgacacct
    13921 tcacgcgagc caactatctg ggcgccgtgg ccgcgggcgt cgactcgttc tgggtgcccg
    13981 accatctcaa cgctctgttt ccccgggcgt tgtggcaacc gaagtactgc ggcggggcga
    14041 agatcctgcc atcggccgac gcatatctgg agccgtggac gatgctcggg catcttgccg
    14101 cgcgcaaccg gattggtcgc cttcgcctgg gggtcggtgt caccgacacc ggacgccgca
    14161 accctgcggt gacggcacag gccgccgcga cagttcagct gctcacccgt ggacgcgcca
    14221 tcctgggcat cggcaccggc gaacgcgaag gcaacgaacc gtacggcgtc gactggtcca
    14281 aacccgtggc ccggttcgaa gaagcgatgg ccaccatccg cgcgctgtgg gactcgaaag
    14341 gcgaactggt aaaccgggat tcgccgtact ttcccttgcg taacgcgctg ttcgacctgc
    14401 cgccctacaa gggcaggtgg ccggagatct ggatcgccgc gcacggcccg cgcatgctgc
    14461 ggaccgtcgg gcggtacgcg gacggattcc ttccggcctt cgcccacaag ccgcaggaat
    14521 acacgcggcg cttggaggtc gttcgggccg ccgcatcgga tgccggacgc gacccgatgg
    14581 cgatcgtgcc ggcgatgtgg ctgcctgtcc tgactggacg caccgcgggc gaaatcgaag
    14641 cggcgctcga atccgaagtc atccgggccg gcgcgctgaa tgcgacggac gagttctttg
    14701 cggagcacgg ccttcagcat ccgttgggcg tcgggttcaa aggcggccaa gacctgatgc
    14761 cgcaagactg ggacgagcag acggcgttgg ccaacatcaa agggatcccg ccgtcgttct
    14821 tacgagagat ggcgttggtc ggcaccccca gcgaggtcat tgaccgcgcg gccgaatggc
    14881 gcgactgcgg cgtgcggtac ttggtcgtgg tcaatggggc gttcatgcag cgcagcctgc
    14941 gcaacgcgat cggagcgacg ctgcccttca tgaaggtcgt gcggggcctc aagaagctct
    15001 gaccagcgca tgtcctaatt tgtgggagcc atggcctaga cgccacgcaa tcggtcccca
    15061 agactggatt cgtgcgatcg gtggtggtcc tcggctaccc gggcgtccag gcacttgacc
    15121 tggtcggccc gttcgaggtg ttcaccggcg ccacgatcta cctcgccagc caggggcgcg
    15181 ccgacgaggg ctacgccgtc tccgtcgtct cccgcaacgg tgaaccggcc accaccctga
    15241 ccggtctcgc cttggtcgct cagccgctgc cggatccacg cgaaccgatc gacacgctgg
    15301 tcctgcccgg cggcatcggg gtcgacgatg cccgtcgtaa cgcggacacc gtcggctgga
    15361 tacaaatcgc gaccgagcat gcccgtcgcg ttgtcagcgt ctgcaccggg tcgttcctcg
    15421 ccgcgcaagc cggcttgatc gacggctgcg tcgcaaccac gcattgggcg ttcgctgga
//
LOCUS       DASRJA010000413        16394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_471829,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000413 DASRJA010000000
VERSION     DASRJA010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16394
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471829"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VFC01_17965"
     CDS             <1..852
                     /locus_tag="VFC01_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN81550.1"
                     /translation="MITNDEVDIATGLGPEDGAGDLGVPFQNNETTALRITGNEAPLN
                     DIRIRQAINYSVNRTGIVKALFRGMGEPAAQLIPEGVVGHNSNLQLWPHDPEKAKQLV
                     DEARAAGVPVDKQIRLIGRTAQFPKITETVEVIQNELTEIGLNVKIEMMDTAGQLQYQ
                     LRPFPDNSGPFIALIMHGNQAGDAAFSVEQYMRTDGAQNGYGSAAFDRTIDAAGELTG
                     QQRQDAYAKLFADEPNEVAQFAYIAHMNGVLAKSARVDYQPNSATGDEMRLADMTFAS
                     ADRTDKQ"
     gene            873..1799
                     /locus_tag="VFC01_17970"
     CDS             873..1799
                     /locus_tag="VFC01_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN81551.1"
                     /translation="MFSFVRRRMYTSALPLIIVLLGVFLLARLTGDPTNLYLPESATP
                     EQREAFAAKNGFDQTLLTQMADYLKGVLHLDFGTSMRTGESASAMALRAFPATLQLAV
                     TTMLLAIVLAVIIGCWAAYRPNSLADRLSSLLSMTAASIPDFWFAITGVWLFAVLLGW
                     LPTSGVDSGLSWILPIATLMIRPLGVLTQVVRGAMVSALSAPYVRLARSKGASDFRVV
                     THHALRNAAAPALTVAGDLAVGLINGAVVVEAIFGWPGIGKLMIDAILQRDFAVLQAA
                     VLLTAVSIFALNIIIDACYALLDARVREPAQV"
     gene            1807..2784
                     /locus_tag="VFC01_17975"
     CDS             1807..2784
                     /locus_tag="VFC01_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN81552.1"
                     /translation="MSTQLDLVPVKPTTAIVGEPESRKRSRWFQLLVNDRVAAAAAVV
                     LGLVFLTAIVGPMLVGGLATHIDLDNSNQAPFTLAHGLANILGTDPLGRSMLARLIVA
                     CRTTLSVAIPAVVISAIVGSAIGMWAGYYRGWRETVAMRVADVIMSFPSLLLAVVVLY
                     VFSPSAANIVLVLAITRIPVYLRTARAESAELQSRLFIDAARTFGASAQSIIWRHVLP
                     VVLPTLLTVATLDFCYVMLAESSLSFLGIGIQPPDISWGLMVSQGRTYLHTAWWLSFF
                     PGLAIVITTVSATILAAWARIATDPGQRWRLTVPQKRLSRFTKTTKALS"
     gene            2781..3800
                     /locus_tag="VFC01_17980"
     CDS             2781..3800
                     /locus_tag="VFC01_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005569233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN81553.1"
                     /translation="MTAVAERRSAKDLDADPVLEVDGLTVDIRTITGTMRAVNDVSFS
                     ARRGETLALLGESGCGKSMTATALVGLLEPVADVVAGSAHLADVDLFSVGRAKRREMA
                     GTELAIVFQDALTALNPLYTVGTQLAEPFRIHYNLSAKEAKSKAVELMARVGIPQPET
                     RMNSYPHQFSGGMRQRLLIAMAVALNPSVLIADEPTTALDVTVQAQIMALLRDLRTEY
                     RMAVVLITHDLALVAEEADRVAVMYAGNIIETGPVAEVFANPQHPYTKGLLNSVPVNA
                     RRGDELKSIGGTPPDLHAIPDGCVYQARCPLARDVCATTRPALDSVGPNRKAACHFPE
                     EVVNV"
     gene            3793..4785
                     /locus_tag="VFC01_17985"
     CDS             3793..4785
                     /locus_tag="VFC01_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZN81554.1"
                     /translation="MSEKLLEVRDLRKSFQVPGKKKLCALDGISLDLGRGETLGLVGE
                     SGCGKSTLARTLLMLERPDSGTVRFDGIDPFSLRGKELLAFRRRVQMVFQDPYASLNA
                     RMSAGDIIAEPWRSHKSLYKSRRDRDMRVRGLLDLVGLGAKAADKYPQEFSGGQRQRI
                     GIARALALDPDVIICDEPVSALDLSVQAQVLNLLNDLQGQLGISYVFISHDLSVVRHV
                     ADRVAVMYLGRIIENGPTEAVFERPNHPYTAALMSAAPTLDGAQRKERILLKGEVPSP
                     LNPPSGCRFRTRCWKATEVCATTTPPSAVDPEIAGHTAECHHPLLQEGSALVPA"
     gene            4782..5504
                     /locus_tag="VFC01_17990"
     CDS             4782..5504
                     /locus_tag="VFC01_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZN81555.1"
                     /translation="MSVLGFCTVAGAVMLASCLQASIGFGIGMLAAPVIALVDPRLIP
                     GTLIMVATLVTLIVAVREREDIDLHGTGWALVGRVPGTIAGALLLATLPERALTVMLA
                     GVVLVGVVLTSVGWIPTPRRRNVVLAGATSGVLGTATSIGGPPMALVWQRNSGARLRG
                     TMSGFFLVGSMMSIAALTLTGAIDGRTVWGFAALVPAAVIGYALSRRINRFLDPKRLR
                     WLAIGASTVGAVVLIGRELLPI"
     gene            5513..6316
                     /locus_tag="VFC01_17995"
     CDS             5513..6316
                     /locus_tag="VFC01_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN81556.1"
                     /translation="MTQEPEIAIRKVKSAVRTVELLEYLAARPDNPARLREISDALDM
                     PRSSAHALLRTLVAQGWVRCDPSGTLYGIGIRALLVGTSYLDSDPYLRLITPFLDDLR
                     QDLDETFHLGRLDGTDIVYLATRESKQYTRASNRVGRRLPAYTTALGKALLAERFGTD
                     RDTHVPAALKPLTANTIVDRPVLDNALDEVRVRGYAADNQENTMGLRCFAVALRYCRP
                     SLDAISCSVPVGRLTPQREREIVEALCTMGDKVSRVVRPLANGDKWFAS"
     gene            6327..7655
                     /locus_tag="VFC01_18000"
     CDS             6327..7655
                     /locus_tag="VFC01_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZN81557.1"
                     /translation="MSTPVVRDMAVIPIAGHDSMLLNLSGAHGPFFTRNLVILTDTDG
                     NTGVGEVPGGEPIRRTLDDARAIVTGRGIGDHHAVLNDVRRAFGDRDAGGRGAQTFDL
                     RVAVHAVTAVESALLDLLGQHLSVPVAALLGEGQQRDRVQALGYLFFVGDRTKTDLAY
                     RSGADEEADDWLRLRHEEALSPDAIVRLAVGARARYGFRDFKLKGGVLPAADEAKAVI
                     ALADRFPDARITLDPNGGWLLEDAVKTCRELTDVLAYAEDPVGPEDGFSGREVMAEFK
                     RATGLPTATNMIATDWRELGHAIRIGAVDIPLADPHFWTMAGSVRVAQLCDAWGLTWG
                     SHSNNHFDVSLAMFTHVAAAAPGEITAIDTHWIWQDGQRITVEPYLIVDGYLSVPDKP
                     GLGVELDMDAVNAAHELYVREGLGSRDDAVAMQYLIPGWTFDSKRPALQR"
     gene            7652..8620
                     /locus_tag="VFC01_18005"
     CDS             7652..8620
                     /locus_tag="VFC01_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HZN81558.1"
                     /translation="MKIVVADANLVPHRERLEAAMPPGAKVYWHVGDAPLDALRDANV
                     YVGSRFTAEMAAVAEKLRLIHVAGAGTDKVAFEALAPDVLVANTFHHEESIAEYVIAA
                     AVMLRRNFLSQDRQLRRGVWATSVYDASIPQAGTLGDAHIGFMGFGHIGRHAWNLFRA
                     FGCSGSAVTGSGRVPECGLAWAGDTRELDRLLTECEVAVVSAPLNEHTEGMIGAEQLR
                     ALGRGGVLINIGRGPLVQERALYDALAGGVIRAAAIDVWYRYPSGDGVSAPSDLPFAE
                     LPNVLMTPHSSGVTRDTFIGRVDDVAANIGRLQRGEPLQNVVFGGR"
     gene            complement(8707..11904)
                     /locus_tag="VFC01_18010"
     CDS             complement(8707..11904)
                     /locus_tag="VFC01_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN81559.1"
                     /translation="MDGSKDRRGALTATGLSCGSCGTELPLNSKFCNECGAPVTQASR
                     SAEYKQVTVLFADVVRSMEIAAVLDTERLREIMTDLVDRVTAVVNRYGGTMDKFTGDG
                     VMALFGAPVALEDHAFRACLAALSIQEEAKRLAVEVQNRDCMALQLRVGLNSGQVIAG
                     EIGSGALGYTAVGEQVGLAQRMESVAAPGGVMLSESTARLVEHAAVLGEPEWVRIKGA
                     DTPVPARRLLGTTSGHVRQRQEASLVGRRWEMSALEAMVDRAIGGRGGVVNVVGPPGI
                     GKSRTAREATAAAAARGVEVFWTFCESHARDVPFHAVTRLLRAGTGVADLHGEAARER
                     AREQVPDADAQDLLLLDDLLGIADPEVPLPAIDPDARRRRLTALINTASLARSEPALY
                     IIEDAQWIDAVSESMLADFLAVIQQTKLMVLITSRPEYEGALKRAPEAQTIALAPLGD
                     SDTAALIGELLGSDPSIGELAAIIAERAAGNPFFAEEMVRELVQRGVLTGTHGKYVCR
                     ADVADVAVPATVQAAIEARIDRLTTEAKRTLNAASVIGASFGPQLLAALGIDAVFDEP
                     LSAELIDQVRFTPDAEYAFRHPLIRAVAYESQLRSDRAELHRRVAAAIESRDPAAADE
                     NAALIAEHLEAAGERKAAYGWHMRAATWATYRDIAAARQSWERARKIADALPAEDLDQ
                     AAMRIAPRTMLCGTAWRVHVNEAGDRFDELRQLCTAAGDKASLAMGMAGLMLDQAWRG
                     RNREASRLASEAMALIESSGDATLTVGLSFAVIYAKSHSADWGDALRWSQRAIDLANG
                     DPFKGNFIIGSPLAVALTSGATARYCLGRPGWHDDLRHGLAMARSTDPLSYAAVVGWC
                     YLPGIPFGVLAAHDRALREIEDALRIAERSGDDMAVAIARGTLGLALVHRHTEAEHSS
                     GQKLLAEVSDALLRRGHGLSELRLINVYLARERARRGDRDEAIPLMFAAVDQLVREGQ
                     LLFWGPVATGVLVETLLDRGAEGDVAEAEAAIERLAAPTDDLPMRDIWLLRVRALLAR
                     ARDDAAAYTHFRDRYAAMATSLGFEGHTAWAEAMP"
     gene            complement(12051..13637)
                     /locus_tag="VFC01_18015"
     CDS             complement(12051..13637)
                     /locus_tag="VFC01_18015"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN81560.1"
                     /translation="MTSPQGGSRLGTRFGPYELNSLIGVGGMGEVYRAYDTVKERTVA
                     VKVLRAEMAADPNYQERFRRESRVAARLQEPHVIPVHDFGDIDGVLYIDMRLVEGGSL
                     KDELRANGPLAPSRAASIIAQMGAALDAAHADGLVHRDIKPENVLLTPDDFAYLVDFG
                     IAHGGGDPSMTTTGLIIGSCAYMSAERFSGGQVGPSADVYSLTCLLYESLTGRPPFET
                     GDLRQLMTAHMFSPPPRPSIMRRGIARAFDDVIAKGMAKKPEERFASAGELAKAARAA
                     VSGGPVPPAPVPAPVPAPAPVPVPSSPPASSTRQFSAVYANPAGTGYTPYPPPEIRPP
                     EPPAPRKSRLSTSQVALIVATVLLFGVAVVLAMQLFSGNDDSAAPPQTTVAVPPPSVS
                     TSTETVEPSSTPSATSTTPTTTTSGAPLPGVSGTDAQGFVGHAARCDDGSTVAAAIRT
                     AQSLAVICETTPGSYYYHGERLRDGANVKLANATPSAGGFDVTNPADGASYEVRPDHL
                     TIRSNGHVDSNEPALEYGSR"
     gene            13865..16144
                     /locus_tag="VFC01_18020"
     CDS             13865..16144
                     /locus_tag="VFC01_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN81561.1"
                     /translation="MLYRIALLAIAAPRRVIASAVLLTVALGIFGIPVAKSLSASGFQ
                     DPTSESARATHVLTDKFHQGDVQLLVVVSTPDGVDSAAARAAGNDIVDQLRSSPHVAQ
                     VTSPWTAPPPAAADLVSRDGRSGLIVAGITGNENQQQNYAKELSDQVAGDHDGVTVRS
                     GGTAMVNVQITEQSQHDLLVMESLAIPLSFLVLVWAFGGLVAAALPVAVGVMAILGAL
                     AVLRLISFATDVSIFALNLTTAMGLALAIDYTLLMISRFRDELADGASRDDALVRTML
                     SAGRTVAFSATTVALSMAVLVLFPMHFLKSFAYAGVATVTFAAAAAIVVTPAALVLVG
                     DRLDALDVRRLARRALGRSEPQPMPVEQRFWYRSASCVMHKAVPLGLAGVALLLLLGT
                     PFLSVRWGFPDDRVLPRSASAHEVGDQLRRDFVHNSDAAVTVVVPDTTGIGAADIDRY
                     AADLSRVPDVSAVSAPTGTFIDGNRVGPPSAPAGQIPGSAFLTVDSSAPLFSDPSETQ
                     LDRLHAVPAPAGKQVDMTGTAQTNRDSVDAITSRLPLVLGLIGVIMFVLLFLLTGSVV
                     MPLKALVLNILSLTAAFGAMVWIFQDGHLGALGTTPTGTLVANMPVLLFCIAFGLSMD
                     YEVFLVSRIREFWLASGKTRADNDESVARGIAHTGRIITAAALIMSISFAALIAANVA
                     VMRLFGLGLTLAVLVDATLVRMVLVPAFMHVMGQWNWWAPRWLVAVHRRLGLNDGQPH
                     ATQEPHSGEIEPTGSEPRV"
     gene            16148..>16394
                     /locus_tag="VFC01_18025"
     CDS             16148..>16394
                     /locus_tag="VFC01_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HZN81562.1"
                     /translation="MSVIAGVHGVLPPNQYSQDEITDAFVAVPAFAGYEQVVRGLHAS
                     SKVDSRHLILPIDQYLTLGDLGETNNLFIEHAVEMGCA"
BASE COUNT         2715 a         5602 c         5261 g         2816 t
ORIGIN      
        1 atgatcacca acgacgaggt cgacatcgcc accgggctcg gacccgagga cggcgcaggc
       61 gatctcggcg tgccgttcca gaacaacgag accaccgcgc tccggatcac cggcaacgag
      121 gcaccgctga acgacatccg catccggcag gcgatcaact actcggtcaa ccgcaccggc
      181 atcgtcaagg cgctcttccg gggaatgggt gaaccggccg cgcaactcat ccccgaaggc
      241 gtcgtcggcc acaactcgaa tctgcagctg tggccgcatg atccggagaa ggccaagcaa
      301 ctcgtcgacg aggcgcgggc cgccggtgtg cccgtcgaca agcagatccg gctgatcgga
      361 cgcaccgcgc aattcccaaa gatcacggaa accgtagagg tcatccagaa cgagctgacc
      421 gagatcggac tcaacgtcaa aatcgagatg atggacaccg cgggccagtt gcaataccag
      481 ctgcggccct tcccggataa cagcgggccg ttcatcgcgc tgatcatgca cggcaaccag
      541 gccggtgatg ccgccttcag cgtcgagcag tacatgcgca ccgacggcgc gcaaaacggc
      601 tatggctcag ctgctttcga tcgcacgatc gatgccgccg gggagctcac gggtcaacag
      661 cgccaagacg cgtacgccaa gttgttcgcc gacgaaccca acgaggttgc ccagttcgcc
      721 tacatcgcgc acatgaacgg tgtgctcgcc aagtcggcgc gcgtcgacta tcagcccaac
      781 tccgcgaccg gcgacgagat gcgacttgcc gacatgacgt tcgcatctgc cgaccgcacc
      841 gacaagcagt gacaagcggg aggccgtaac cgatgttctc gttcgttcgc cgccggatgt
      901 acaccagcgc attgccgttg atcatcgtgc tgctgggtgt cttcctgctg gcccgtctca
      961 ccggcgaccc gaccaacctg tatctgcccg agtccgcgac cccggagcag cgcgaggcgt
     1021 tcgccgccaa gaacggtttc gaccaaacgc tgctcacaca gatggccgac tacctgaagg
     1081 gtgtcctcca tctcgacttc ggcacgtcga tgcgcaccgg cgagtccgcg tccgcgatgg
     1141 cactgcgcgc tttcccggcc acccttcaat tggccgtcac cacaatgctt ctggcgatcg
     1201 tgcttgcggt gatcatcggc tgctgggcgg cgtatcgccc gaactcgctg gccgaccggt
     1261 tgtccagcct gctgtcgatg accgccgcga gcatccccga cttctggttc gccatcaccg
     1321 gcgtctggct gttcgcggtc ctgctaggct ggttgcccac atccggcgtc gacagcggcc
     1381 tgtcatggat cctgcccatc gccacgctga tgattcgtcc gctcggcgtg ctgacccaag
     1441 tcgtccgcgg cgcaatggtt tccgcgctgt ccgcgccgta cgtgcggctg gcccgcagca
     1501 agggcgccag cgactttcgt gtcgtcaccc accatgcgct ccgcaacgcc gccgccccgg
     1561 cactgaccgt tgccggcgac ctggccgtcg ggctgatcaa cggtgcggtc gtggtcgagg
     1621 cgatcttcgg ctggcccggc atcggcaagc tgatgatcga cgcgattctg cagcgcgact
     1681 tcgccgtcct gcaggctgcc gtactgctga ccgctgtgag catcttcgcc ctcaacatca
     1741 tcatcgacgc ctgttacgcg ttgctcgacg cgcgggtgcg ggaaccggca caggtctagg
     1801 agacatatga gcacccaact cgatctcgtc ccggtcaagc cgaccaccgc gatcgtcggc
     1861 gagcccgaaa gccgcaagcg ctcaaggtgg ttccagttac tggttaacga ccgcgttgcc
     1921 gccgcggccg ccgtcgtact ggggctcgtg ttcctgactg cgatcgtcgg cccgatgctc
     1981 gtcggcggcc tcgccaccca catcgacctc gacaactcca accaggcgcc gttcacgctc
     2041 gcgcacggct tggcgaacat cctcggcacc gacccgctgg gccgcagcat gctcgcccgc
     2101 ctcatcgttg cctgccgcac caccttgtcg gtggcgattc ccgccgtcgt gatttccgcg
     2161 atcgtgggct cggcgatcgg catgtgggcc ggctactacc gcggctggcg cgagaccgtg
     2221 gccatgcgtg tcgccgacgt gatcatgagc ttcccgtcgc tgctgctcgc cgtcgtcgtg
     2281 ctctacgtgt tctcccccag cgccgcgaac atcgttctgg tgctggcgat cacccgcatc
     2341 ccggtctacc tgcgcaccgc gcgcgccgag tccgccgaac tgcagagccg gttgttcatc
     2401 gacgccgcac gcacgttcgg cgccagcgcc caatccatca tctggcgcca cgtcctgccc
     2461 gtcgtgctgc cgacgttgct gaccgtcgcg acgctcgact tctgctacgt catgctcgcc
     2521 gagtcttcgc tgagcttcct gggaatcggc attcagccgc ccgacatcag ctggggcctg
     2581 atggtgtcgc agggccgcac gtatctgcac accgcgtggt ggctgtcgtt cttcccaggc
     2641 ctggccatcg tgatcaccac ggtgtcggca accatcctcg ccgcctgggc ccgcatcgcc
     2701 accgaccccg gccagcggtg gcgcctgacc gttccgcaga aacgcctgtc ccgcttcacc
     2761 aagaccacga aggccctctc atgacagctg tcgcagaacg ccgctccgcc aaggatctgg
     2821 acgccgaccc ggtgctcgaa gtcgacggcc tgaccgtcga tatccgcacc atcaccggca
     2881 ccatgcgcgc cgtcaacgac gtcagctttt cggctcgtcg cggcgaaaca ctggcgctgc
     2941 taggcgaatc cggctgcggc aagtcgatga cggccaccgc gcttgtcggg ctgctggagc
     3001 cggtcgccga cgtcgtcgcc ggttcagcgc acctcgcgga cgtcgatctg ttcagcgtcg
     3061 gccgggcgaa gcgccgcgag atggcgggca ccgaactcgc gatcgtgttc caagacgcgc
     3121 tcactgcact caacccgctc tacaccgtgg gaacccaact ggccgaaccg ttccggatcc
     3181 actacaacct gagcgccaag gaggccaagt ccaaggccgt tgaactgatg gcccgcgtcg
     3241 gcatcccgca gccagagaca cggatgaact cctatccgca ccagttctcc ggcggcatgc
     3301 ggcagcgact gctcatcgcg atggccgtcg cgctcaatcc cagcgtgctg atcgccgacg
     3361 agcccaccac cgcgctcgac gtcaccgtgc aggcccagat catggcgctg ctgcgcgacc
     3421 tgcggaccga ataccggatg gccgtcgtgc ttatcaccca cgacctggcg ctggtcgccg
     3481 aggaggccga ccgcgtcgca gtgatgtacg cgggcaacat catcgaaacc ggcccggtgg
     3541 cagaggtttt cgctaatccg cagcatccct acaccaaggg cctgctgaac tccgtcccgg
     3601 tcaacgcgcg tcgcggcgac gagctcaagt ccatcggcgg cacgccgcca gacctgcatg
     3661 cgatcccgga cggctgcgtg taccaggcgc ggtgtccgct ggcgcgtgac gtctgcgcga
     3721 ccacgcggcc cgctctggat tcggtcggac ccaaccgcaa ggccgcctgc cactttcccg
     3781 aggaagtagt aaatgtctga gaagctgctc gaggttcgtg atctgcgcaa gtcgttccag
     3841 gtccccggaa agaagaagct gtgcgcgctt gacggcatca gcctcgactt gggccgcggc
     3901 gagacgctcg gcctggtcgg cgagtccggc tgcggaaagt cgacgctggc aagaacattg
     3961 ctgatgctgg agcgccctga ctccggcacg gtgcggttcg acggcatcga cccgttcagc
     4021 ctgcgcggca aggagctgct ggcgttccgc cgccgcgtgc agatggtgtt ccaggatccg
     4081 tacgcctcgc tgaacgcgcg catgtcggcg ggcgacatca tcgccgagcc gtggcgcagc
     4141 cacaagagtc tgtacaagag ccggcgcgac cgcgacatgc gggtgcgggg gctgctggat
     4201 ctcgtcggcc tcggcgcgaa agcggcggac aagtacccgc aggagttctc cggcggccag
     4261 cgccagcgca tcggcatcgc ccgggcattg gcgctcgacc ccgacgtgat catctgcgac
     4321 gagccggtgt ccgcgctcga tctatccgtg caggcgcagg tgctcaacct gctaaacgac
     4381 cttcagggtc agcttggcat ctcctacgtc ttcatctcgc acgacctgtc ggtcgtccgg
     4441 catgtcgccg accgcgtcgc ggtgatgtac ctcggccgga tcatcgaaaa tggccccacc
     4501 gaagcggttt tcgagcggcc caaccatccg tataccgcag cactgatgtc tgcggcgccg
     4561 acgctcgacg gcgcccagcg caaggagcgg atcctgctga agggcgaggt gccgtcgccg
     4621 ctcaacccgc cgtcgggttg ccggttccgg acgcgttgct ggaaagccac cgaggtgtgc
     4681 gcgaccacca caccaccgag tgccgtcgac ccagaaatcg cgggacatac cgccgagtgc
     4741 caccatccct tgcttcagga ggggagcgcg ctggtgccgg catgagtgtc ctcggatttt
     4801 gcacggtcgc cggcgcggtc atgctggcgt cgtgtctgca ggcgtcgatc ggattcggga
     4861 tcggcatgtt ggccgcgccg gtcatagcgc tcgtagaccc tcggctcatc ccgggcacgc
     4921 tgatcatggt ggcgacgctc gtcacgctga tcgtcgcggt acgcgaacgc gaggacatcg
     4981 acctgcacgg caccggctgg gcgctggtgg gcagggtgcc cggaaccatc gcgggcgcac
     5041 tgcttttggc gacgttgccc gagcgggcgc tgacggtcat gctcgcgggg gtggtgctcg
     5101 tcggcgtggt cctcaccagc gtcggatgga ttccgacccc gcgacggcgc aacgtggtgc
     5161 tggccggggc gacctcgggc gtgctcggca ccgcgacgtc gatcggcggg ccaccgatgg
     5221 cgctcgtgtg gcaacgcaac agtggcgctc ggttgcgtgg cacgatgagc ggctttttcc
     5281 ttgtcggatc gatgatgtcg atcgccgcgc tcacactgac cggcgccatc gacggccgaa
     5341 ccgtgtgggg cttcgcagca ctggtaccgg cggcggtgat cggatacgca ctgtcacgcc
     5401 gcatcaaccg ctttctcgat ccgaaacgac tgcgctggtt ggcaatcggc gcctccaccg
     5461 tgggtgccgt cgtgttgatc ggccgcgaac tgctgccgat ctgaggagaa ctgtgacaca
     5521 agagcccgag atcgcgatcc gcaaggtgaa gtccgccgtg cggaccgtcg agttgctcga
     5581 gtatctggcg gcccgccccg acaacccggc gcgcctgcgc gagatcagcg acgcgctgga
     5641 catgccgcgc agcagcgcgc acgccctgct ccgcacgctc gtcgcgcagg gctgggtgcg
     5701 gtgtgacccg tcaggcacgt tgtacggcat cggtattcgt gcactgctcg tcgggaccag
     5761 ttatctggac agcgacccgt atctgcggtt gatcacgccg ttcctcgacg atctgcgcca
     5821 ggatctcgac gagacgtttc acctcggccg gctggacggc acggacatcg tgtacctggc
     5881 gacacgcgaa tccaagcagt acacccgcgc atcgaatcgc gttggcaggc ggctgcccgc
     5941 ctacaccacc gctctgggca aggcgctgct ggccgagcgg ttcggcaccg accgcgacac
     6001 tcacgtccct gccgcgctga agccactgac cgccaacacg atcgtcgacc ggcccgtgct
     6061 ggacaatgcg ctcgatgaag tgcgggtgcg ggggtacgcc gccgacaacc aggagaacac
     6121 catggggctg cggtgcttcg cggtggcact gcgctactgc cggccctcgc tggatgccat
     6181 cagctgctcg gtaccggtgg ggcggctgac accgcagcgt gagcgcgaga tcgtcgaggc
     6241 actgtgcacc atgggcgaca aggtgtcccg cgtggtgcgg ccgctggcca acggcgacaa
     6301 atggttcgcg tcgtgaggag attgacatga gcacacccgt cgtgcgcgac atggccgtga
     6361 ttccgatcgc cggccacgac agcatgctgc tgaacctcag cggcgcgcac gggccgttct
     6421 tcacccggaa cctggtgatc ctcaccgaca cagacgggaa caccggcgtc ggcgaggttc
     6481 cgggagggga gccgatccgg cgcaccctcg acgatgcccg cgcgatcgtc accggccgcg
     6541 gcatcggcga tcaccacgcc gtgctcaacg acgtgcgccg cgccttcggc gatcgggatg
     6601 cggggggacg cggcgcgcag acgttcgacc tacgcgtcgc cgtgcacgcg gtgaccgcgg
     6661 tcgagtcggc gctgctggac cttctcgggc agcacctttc agtgccggtg gcggcgctgc
     6721 tcggtgaggg ccagcagcgt gaccgcgtgc aggcgctcgg ctatctgttc ttcgtcggcg
     6781 accgcaccaa gacggatctc gcgtaccggt cgggggcgga cgaagaggcc gacgattggc
     6841 ttcgcctgcg gcatgaggaa gcgctgtcac ccgacgccat cgtccggctc gccgtgggcg
     6901 cgcgggcgcg gtacggcttc cgcgacttca agctcaaggg cggggtactg ccggcagctg
     6961 acgaggccaa ggcggtgatc gcgctggccg accggttccc ggatgcccga atcacgttgg
     7021 accccaatgg cggttggcta ctcgaggacg cagtgaagac gtgccgcgag ctgaccgatg
     7081 tgctcgccta cgccgaagac cccgtcgggc ctgaggacgg cttctcgggt cgcgaggtga
     7141 tggccgaatt caaacgggcg accggcctac cgacggcgac gaacatgatc gccacggatt
     7201 ggcgtgaact gggacatgcg attcgcattg gcgcggtcga tatcccgttg gcggatccgc
     7261 acttctggac gatggccggt tcggtgcgcg tcgcgcagct gtgcgacgcg tggggtctga
     7321 cgtggggttc gcactccaac aatcacttcg acgtatcgct ggcgatgttc actcacgtcg
     7381 ccgccgcggc accgggagag atcaccgcga tcgacactca ctggatctgg caggacggac
     7441 agcggatcac cgtcgagccg tatctgatcg tcgacggtta tctgagcgtg cccgacaagc
     7501 cgggcctggg tgtcgagctc gacatggacg cagtcaacgc cgcacacgag ctgtatgtac
     7561 gcgaagggct gggcagccgc gacgacgctg tggcgatgca gtacctgatc cctggttgga
     7621 cgttcgacag taaacgacct gcgctgcagc gatgaagatt gttgtcgcgg acgcgaattt
     7681 ggtcccgcac cgcgagcgac tcgaggctgc gatgccaccg ggcgccaagg tgtactggca
     7741 tgtgggtgac gcgcccttgg acgcgctgcg ggacgccaat gtgtatgtcg ggagccggtt
     7801 caccgcggaa atggccgccg tcgcggagaa gctgcggctg attcatgtcg ccggggcagg
     7861 caccgacaag gtggcgttcg aggcgttggc gccggacgtg ctggtggcca acacgtttca
     7921 ccatgaggag tcaatcgcgg agtatgtgat cgccgcggcg gtgatgctgc ggcgcaactt
     7981 cctgagtcag gaccggcagc tgcgtcgtgg cgtctgggca acatcggtgt acgacgcgtc
     8041 gataccgcag gcgggcacgc tcggcgatgc acacattggg ttcatgggct tcgggcacat
     8101 cggccggcat gcgtggaacc tgttccgcgc cttcggttgt agtggatcgg cggtgacggg
     8161 ctctggccgc gtcccggagt gcggcctggc atgggcgggt gacacgaggg agctggatcg
     8221 gctcttgacg gagtgcgaag tcgcggtggt atcggcgccg ctgaacgagc acacggaagg
     8281 catgatcggc gcggagcagt tgcgcgcgct cggcagaggc ggggtgttga tcaacatcgg
     8341 caggggtccg ctggtgcagg agcgggcgct gtacgacgcg ttggcgggtg gcgtgattcg
     8401 ggcggcggcg atcgacgttt ggtatcgcta cccgtcgggg gacggtgtca gcgcgccgag
     8461 cgacctgccg ttcgcggagt tgccgaatgt gttgatgacg ccgcactcgt cgggggtcac
     8521 gcgcgacacg ttcatcggcc gggtcgacga cgtcgcggcc aacatcgggc ggctgcagcg
     8581 gggagagccg ctgcagaatg tcgtgttcgg tggaaggtga cgacactctt tcgcgcgccg
     8641 tcatccaaat gttgacgccc ttcacctgac gccttccgag acagacaatc tccggccagc
     8701 ccgcaatcat ggcatcgctt cggcccatgc ggtatggccc tcgaagccaa gcgatgtcgc
     8761 catcgcggcg tagcgatccc ggaagtgcgt gtaagccgcg gcatcgtcgc gagcccgggc
     8821 cagtagagca cgtactcgca agagccagat gtcgcgcata ggtaaatcgt cggtcggggc
     8881 cgctaaccgc tcgatagcgg cctcggcttc agccacgtca ccctcggccc cacggtccag
     8941 cagtgtctcc accagaacac ccgtcgcaac agggccccag aacagcagtt gtccctcgcg
     9001 aaccagttgg tcgacggcgg cgaacatgag cggtatcgcc tcatcgcgat ctccacgccg
     9061 agccctctca cgtgccaagt acacgttgat cagccgcaac tcgcttaggc cgtgtccccg
     9121 gcgcagtaac gcgtcgctga cctcggctag gagcttctgt ccgctggaat gctccgcctc
     9181 cgtgtggcgg tgcaccagcg caaggcccag cgttccccgg gctatggcca cagccatatc
     9241 atcaccggat cgttcggcaa tccgtagcgc atcctcgatc tcgcgcagcg cgcgatcgtg
     9301 ggcagccagc acgccaaacg gtattcccgg taagtaacac cagccgacga ccgcggcgta
     9361 ggacaacggg tcggtgctgc gggccatggc caggccgtgg cgaaggtcgt cgtgccatcc
     9421 gggacgaccc aggcaatacc gcgccgtagc ccctgaggtc aaggcaacag ctaacggaga
     9481 cccgattatg aagttccctt tgaacggatc accgttggcc aggtcgatgg cccgctgcga
     9541 ccaccgcagc gcgtcacccc aatcggcgct gtgactcttg gcgtagatca ccgcgaagga
     9601 cagccccacc gtcaacgtcg catcgccgct cgactcgatg agggccatgg cttccgatgc
     9661 cagccgcgac gcctcgcggt tccggccccg ccacgcttga tccaacatca gcccggccat
     9721 gcccatggcc agcgacgcct tgtccccggc ggcggtgcac aactgtcgca attcatcgaa
     9781 acggtccccg gcttcgttca catggactcg ccaggcggtc ccgcacaaca tggtgcgcgg
     9841 ggcgatgcgc atggctgcct ggtcgaggtc ctcggcgggc agtgcgtcgg cgatctttcg
     9901 ggcgcgctcc caactttgtc gcgccgcagc gatgtcgcgg taggtcgccc acgttgcggc
     9961 gcgcatgtgc cagccatagg ccgccttcct ctcgccggcg gcctccaggt gttcggcaat
    10021 caaggcggca ttttcatcag ccgctgctgg gtcacgtgat tcgattgcgg ccgcgacgcg
    10081 ccgatgcagc tcggcgcgat cggatctcag ctgcgattcg taggccaccg cgcgaatcag
    10141 cggatgacgg aaggcgtact cggcgtccgg ggtgaaccgc acctgatcaa tcagctccgc
    10201 gctgagcggc tcgtcgaaca ccgcatcgat gcccagcgcg gcgagcagct gcggcccgaa
    10261 gctggctccg atcaccgacg ccgcgttcaa cgtccgcttg gcttcagtgg tcaaccggtc
    10321 gatgcgcgcc tcgatggccg cctgcaccgt ggccggcacc gcgacgtcgg cgacatccgc
    10381 gcgacagaca tacttaccgt gcgtgccggt aagcaccccg cgctgtacca actcgcgcac
    10441 catctcctcg gcgaaaaacg ggttccccgc ggcccgctcg gcgatgatcg ccgccagctc
    10501 gccgatcgag ggatccgaac ccagcagctc accgatcagc gccgcggtgt ccgaatcacc
    10561 caggggggca agggctatcg tctgcgcttc cggcgcccgc ttgagcgctc cctcatattc
    10621 ggggcgggag gtgatcagca ccatcaactt ggtctgctgg atcaccgcaa ggaagtcggc
    10681 cagcatcgac tcgctgaccg catcgatcca ctgcgcgtcc tcgatgatgt acagcgccgg
    10741 ttcgctgcgg gccagcgacg cggtgttgat gagtgcggtc aaccggcgcc gccgcgcatc
    10801 cgggtcgatg gcgggcagcg gcacctcggg gtcggcgatg cccaacagat catcgagcag
    10861 cagcagatcc tgcgcgtcgg catcggggac ttgttcccgg gcccgttcgc gggcggcttc
    10921 gccgtggagg tcagccacgc cggtgcccgc gcgcagtagt cgggtcacgg catggaacgg
    10981 gacgtcacga gcatgcgact cgcagaaggt ccaaaacacc tcgaccccgc gagcggccgc
    11041 ggccgccgtg gcctcccgcg ccgtccggga cttgccgatg cccggcggtc ccaccacatt
    11101 caccacacca ccgcggccac cgatcgcgcg atccaccatg gcctccagcg ccgacatctc
    11161 ccagcgccga ccgaccagac tggcttcttg gcgctgcctc acatggcctg aagtcgtgcc
    11221 aagcaaccgg cgcgcgggga caggcgtgtc ggcacctttg atgcgcaccc attccggttc
    11281 acccaataca gcggcatgct cgaccagccg ggcggtggac tcgctgagca tcaccccacc
    11341 cggcgccgcg accgattcca tccgctgcgc caacccgacc tgctcaccaa ctgcggtgta
    11401 acccaatgcg cccgaaccga tctcacccgc aatgacctga ccggagttca gcccgacccg
    11461 cagctgcagc gccatacagt cgcggttttg gacttcgacg gcgagccgct tcgcctcctc
    11521 ctgaatcgat agcgccgcaa ggcaggcacg aaaagcgtga tcctccaaag ccactggggc
    11581 cccaaacaac gccatcacgc cgtcgccggt gaacttgtcc atcgtcccgc cgtagcggtt
    11641 tacgacagcc gtaacgcggt ccaccagatc ggtcatgatc tcgcgcaacc gctccgtatc
    11701 cagcacggcc gcgatctcca tcgaccgaac cacatcggcg aacagcaccg tcacctgctt
    11761 gtactccgcc gatcggctgg cctgtgtgac cggtgcgccg cattcgttac agaacttgga
    11821 gttcagcggc agctccgtgc cgcacgaccc acacgacaga cccgttgctg tcagtgcacc
    11881 gcgacggtcc ttgctgccgt ccacgtcagt atcgtttcac cccggctcag gggtgtccgt
    11941 catatgcggc ggatccggca tcttcttgcg gcgagtgtgg ggttaaggca cgttttcctg
    12001 attcgaagcg tgtctcgagc ccacactcgt cgttagagat gaggacgaac tcagcgggac
    12061 ccgtactcca acgccggctc attggaatcc acatgaccgt tgctgcggat cgtcagatga
    12121 tcgggccgca cctcgtagct agcgccgtca gccgggttgg tcacgtcgaa tccaccggcg
    12181 gacggcgtcg cgtttgccag tttcacgttg gcgccatccc tcaatcgttc gccgtggtag
    12241 tagtagctcc ccggcgtcgt ctcacagatc accgccagcg actgcgcagt cctgatcgca
    12301 gccgcgacag tgctcccgtc atcgcagcgg gcggcatggc cgacgaagcc ctgcgcatcg
    12361 gttcctgaca cgccgggcag cggtgccccg ctcgtcgttg tggtcggtgt ggtggatgtg
    12421 gccgatggtg tcgatgacgg ctcgaccgtc tcggtcgacg tgctcaccga cggcggcggc
    12481 acggccaccg tggtctgcgg cggcgcggcg ctgtcgtcat tgcccgagaa cagctgcatc
    12541 gccagcacca ccgcgacgcc gaacagcagg accgtcgcca cgatcagcgc cacctgactg
    12601 gtgctcagcc gcgatttgcg cggcgccggc ggctccggcg gccgaatctc cggcggcggg
    12661 tacggcgtgt atcccgtgcc cgccggattg gcgtacaccg ccgagaactg tcttgtcgac
    12721 gacgccggcg gcgaactcgg cacaggaaca ggagcaggag ccggcacagg cgcaggcaca
    12781 ggcgcaggcg gcacaggacc gccggacacc gccgccctcg ccgccttggc caactcgccc
    12841 gcagacgcga accgttcctc cggcttcttg gccatgcctt ttgcaatcac gtcgtcaaac
    12901 gcgcgtgcaa tgcctcggcg catgatgctt ggccgcggcg gcggcgagaa catgtgcgcg
    12961 gtcatcagct gccgcagatc tcccgtctca aacggcggcc gcccggtgag gctttcgtag
    13021 agcaggcagg tcagcgagta cacgtccgcg gacggcccga cctgcccacc gctgaacctc
    13081 tccgccgaca tgtacgcgca cgagccgatg atcagccccg tcgtcgtcat gctcgggtcg
    13141 ccgccgccgt gggcgatccc gaagtccacc agatacgcga agtcatccgg tgtgagcagc
    13201 acgttctccg gcttgatgtc acggtgcacc aggccgtccg catgcgccgc gtccagcgcg
    13261 gcccccatct gcgcgatgat cgacgcggcc ctgctcgggg cgagcgggcc gttcgcacgc
    13321 aactcgtcct tcaggctgcc gccctcgacc agccgcatgt cgatgtacag cactccgtcg
    13381 atgtcgccga agtcgtgcac agggatgacg tgcggttcct gcaatcgcgc agctacccgc
    13441 gactcgcggc ggaagcgttc ctggtagttc gggtccgcgg ccatctccgc gcgcagcacc
    13501 ttgaccgcca ccgtgcgctc cttgacggtg tcgtaggcgc ggtacacctc gcccatcccg
    13561 ccgacaccga tcagagaatt cagctcgtag ggcccaaaac gggtgcccag gcgcgagcca
    13621 ccctgtgggg aggtcatgca aatcctttcc gttcgaaccc cgacgcgcag caatacaccg
    13681 tgttcccaca ttggatgcac gcttaacgtg ctcgaagttc gaagcgcagc aaagcaactg
    13741 gcttccgacg cacgcaggta ccacactacg gtgcgctgac cagtgccgca taactcgata
    13801 gattgaacga ctaaccgacg tctttcgttc taggtactac agaggactga gcggaggaca
    13861 ggcgctgctg tatcggatcg ccctgctggc cattgccgca ccgcgacggg tcatcgcatc
    13921 cgcggtgctg ctgactgtcg cccttggaat attcggaata cccgttgcga agagcctgtc
    13981 cgccagtgga ttccaggacc ccacgtccga atcagccagg gcgacgcacg tcctgaccga
    14041 taagttccat cagggcgacg tgcaattgct ggtcgtcgtg tcgacacccg acggcgtcga
    14101 cagcgcagca gctcgcgcag caggcaacga catcgtcgat caactgcgca gttcgccaca
    14161 tgtcgcgcag gtgacatcgc catggacagc gccgccgccc gccgccgccg atctggtcag
    14221 ccgcgacggt aggtcgggcc tgattgtggc gggcatcacg ggtaacgaga atcagcagca
    14281 gaattacgcc aaggaattgt ccgaccaggt ggcgggtgac cacgacggtg tcaccgtccg
    14341 ctcgggtggc accgcgatgg tcaatgtgca gatcaccgaa cagtcgcagc acgacctgct
    14401 ggtgatggag tcgctggcga tcccgctgag ttttttggtg ctggtctggg cgttcggcgg
    14461 gctggtggcc gccgcgctgc cggtcgcagt tggcgtgatg gcgatcctcg gcgcgctcgc
    14521 tgtactgcgg ttgatttcgt ttgccaccga cgtgtcgatc ttcgcgctca acctcaccac
    14581 cgcgatgggt ctggcgctgg cgatcgacta caccctgctg atgatcagcc ggttccgcga
    14641 cgagttagcc gacggcgcga gccgcgacga cgcccttgtg cgcacgatgc tgtccgccgg
    14701 ccgcaccgtg gcgttctccg ccacgacggt cgccctgtcg atggcggtcc tcgtgctctt
    14761 cccgatgcac ttcctgaagt cgttcgccta cgcgggtgtc gcaactgtga cgttcgccgc
    14821 ggctgccgcg atcgtggtga cacccgctgc gctggtcctg gtcggcgacc gcctcgatgc
    14881 gctggacgtg cgccggctcg ctcgccgcgc gcttggccgc tccgaaccgc aaccgatgcc
    14941 cgtcgagcag cggttctggt accgatccgc gagttgcgtt atgcacaaag cagttccgct
    15001 cggattggcg ggtgtcgcgt tgctattgct gctcggcacg ccgttcctga gcgtgcgctg
    15061 gggattcccc gacgatcgcg tgctgccgag atcggcatcc gcacatgaag tcggtgacca
    15121 gttgcggcgc gacttcgtgc acaactccga cgccgcggtg accgtcgtcg tccccgacac
    15181 caccggcatc ggcgcagctg acatcgacag gtacgcagcc gacctttctc gcgtacccga
    15241 cgtgtccgcc gtgtcggctc cgacaggaac gttcatagac ggcaaccggg ttggcccgcc
    15301 ctcggcgccc gcaggacaga ttcctggcag cgccttcctg accgtcgaca gctcggctcc
    15361 gctgttctcc gacccgtcag aaactcagct cgaccgcctg catgccgtac ccgcccccgc
    15421 aggcaagcag gtcgatatga ccggcaccgc gcagacgaac cgcgacagcg tcgacgccat
    15481 caccagtcgg ctgccgctgg tgctcgggct catcggcgtc atcatgttcg tactgctgtt
    15541 cctgctgacc ggcagcgtcg tcatgccgct gaaagcgttg gtgctcaaca tactttcgct
    15601 gacggccgcg ttcggtgcga tggtgtggat cttccaggac ggccatctcg gcgcgctcgg
    15661 caccacaccg accggcacgt tggtggccaa catgccggtg ctgttgttct gcatcgcgtt
    15721 cggcctgtcg atggactacg aagtgttcct cgtttcacgg atcagggaat tctggctggc
    15781 gtcaggcaag acgcgggccg acaacgacga gagtgtcgcc cgcggcatcg ctcacaccgg
    15841 gcggatcatc accgccgccg cgctgatcat gtcgatttcc ttcgccgcgt tgatcgctgc
    15901 gaacgtggcg gttatgagac ttttcgggct cggcctgacg cttgcggtgc tggtcgacgc
    15961 cacgcttgtg cggatggtgc tggtgcccgc gttcatgcac gttatgggcc aatggaattg
    16021 gtgggcgccg cggtggttgg tcgcggtgca ccgacgactc ggtttgaatg acggtcagcc
    16081 gcatgcgaca caggaaccgc acagcggcga gatcgaaccg accggctccg aaccccgtgt
    16141 ctagagtgtg agcgttatcg caggcgtaca tggagtcctt ccgccgaatc aatacagcca
    16201 ggatgagatc accgacgcgt tcgtcgcggt tcccgccttc gccggctacg agcaggtggt
    16261 gcgcggactg cacgccagct cgaaggtcga cagccgacac ctgatccttc cgatcgatca
    16321 gtacctgacg ctgggcgacc tcggcgaaac caacaacctg ttcatcgagc acgcagtgga
    16381 aatgggctgc gcag
//
LOCUS       DASRJA010000414         7696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3105921,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000414 DASRJA010000000
VERSION     DASRJA010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3105921"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFC01_18030"
     CDS             complement(<1..501)
                     /locus_tag="VFC01_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HZN81563.1"
                     /translation="MAALGAHREVLIVGRLNPMHASVRTRSALAAAVVMTLSLAIAGG
                     VLLLVLYRSLESTAQGAAAARAEQISAQLRTDTPREVDPSLLVTDSQIGAVQIVDDTG
                     TVLAASNGAPTSPLVTTSLSDGQARSVGRVEGPGRSFDYWVSARATAVPGGSVTILVG
                     ADREPVE"
     gene            1003..1134
                     /locus_tag="VFC01_18035"
     CDS             1003..1134
                     /locus_tag="VFC01_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81564.1"
                     /translation="MSMKVNKYITPLLGGTAAAVAMAAPTASAADHQSCNGSGSRTV"
     gene            complement(1565..1816)
                     /locus_tag="VFC01_18040"
     CDS             complement(1565..1816)
                     /locus_tag="VFC01_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81565.1"
                     /translation="MGMRLQYITPLLAAGAAAVAIVAAPTAFATDQQSCNGSGSGTVC
                     LSPGNVPIKTSPADFQHHPSGDMPVLTVQDFALALALVT"
     gene            complement(2558..2794)
                     /locus_tag="VFC01_18045"
                     /pseudo
     CDS             complement(2558..2794)
                     /locus_tag="VFC01_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(3315..3599)
                     /locus_tag="VFC01_18050"
     CDS             complement(3315..3599)
                     /locus_tag="VFC01_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HZN81566.1"
                     /translation="MFAGLLGIVLLLFELVLIARMVVDWIGVLSPVGGRTGLYQARRI
                     THGITEPVIAPVRRVLKPARFGSFSIDLAFTAVFVAVIVLRTFVVPLIPF"
     gene            3770..5032
                     /locus_tag="VFC01_18055"
     CDS             3770..5032
                     /locus_tag="VFC01_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81567.1"
                     /translation="MSETTSSDAVKTSRVLLSKVPEVTVWFWVIKILCTTVGESFADW
                     INMTLGVGLTQTSVIFTVVLAAVLGWQLSLNRYVPFVYWLTVVLLSVTGTLYTDILTD
                     DLGVPLALSTSVFAVILAIVFGVWFARERTLSIHSIVTRPRELFYWLAVLVTFALGTA
                     AGDWILELTGWGPGTSVLLPAGLIVAIVIGWRLGANAVLSFWLAYILTRPLGANLGDW
                     LGFPSDQRGLGLGVALTSAIFLTAILAIVLYLTRTRADVIEESDQNRPRTATKNAARE
                     RIMLGYYAVVAAATGTLLVWAAGQPHQTATASENESGGSSVTVTLAPGESATANFPPA
                     EVAKFRTIAADTLAQVRAGDQSGATARIKDLETAWDDDQPTLQPMDDIAWTVLDGQID
                     NVLTALRASSPDPATETQTLSALLTSLR"
     gene            5248..6030
                     /locus_tag="VFC01_18060"
     CDS             5248..6030
                     /locus_tag="VFC01_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81568.1"
                     /translation="MTNTALFLAVFLACAVEAVEALTIILAAGTARDWRSANTGLLAG
                     LVTLAVVVAALGPALSAIPLDGLRVFVGGVLLVFGLQWLRKAILRASGHKAPHDEAML
                     FEATLATARQAGTKRRGMAIDWYAFTLSYKGVVLEGLEAAFIALTFGANQHNIPLAAA
                     AAVAAVLVVASVGFAVRAPLSRVPENSMKFIVGVMLIAFGMFWAAEGAGAHWPLDDAA
                     LLVLIPAVTLYALALVALFRRQAAIASPVNADAAITEEATRS"
     gene            6027..6236
                     /locus_tag="VFC01_18065"
     CDS             6027..6236
                     /locus_tag="VFC01_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81569.1"
                     /translation="MIRRIRAFGAFWYDFIIGDDWVVALGVALALAITFAVSRSTDAP
                     VWWIIVVAVAILLPLSVFRATRGRR"
     gene            complement(6242..6934)
                     /locus_tag="VFC01_18070"
     CDS             complement(6242..6934)
                     /locus_tag="VFC01_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HZN81570.1"
                     /translation="MLSPTDFGAVANINSGCTTRRQQLSACDVGQTDAVGDAGRGAEE
                     PAGSAHTDPDPAGWLLLVYRIPSDPTRLRATVWRRLKSLGAVYLQNSAAALPATDGAE
                     RALRKLRREILEMNGSAVLLSCSAVAGEQDVVALFQAARDDEYDEILDKCRDFHAGLD
                     KEYRANHFTYGELEENEVELVKLKNWYAKVAARDVFGTRNRGAAVEALDACEQALERY
                     AARVYAEQDEGG"
     gene            complement(7042..7536)
                     /locus_tag="VFC01_18075"
     CDS             complement(7042..7536)
                     /locus_tag="VFC01_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN81571.1"
                     /translation="MSPRPATNGMGCTAHSRKPRPAQYRLEFDGGSTIAVRGRGLIGR
                     YPVAAADKNVEHLVALADDTITMSRTHLEFDIGESGLWVRDCASTNGSQIEVDGHRTA
                     MEPGLPVHAPSGYTIHMGGRRVKVLTILSHSAIDPQINWGVATHTGIVCETNQDAYCT
                     TPTM"
BASE COUNT         1290 a         2530 c         2426 g         1450 t
ORIGIN      
        1 ttcaacgggt tcccggtcgg cgccaaccag gatggtcacc gacccgccgg gaaccgcggt
       61 cgcacgggcc gacacccagt aatcgaagct acgcccagga ccttccaccc gtcccacgct
      121 gcgcgcctga ccatcgctca atgaggttgt caccagcggt gatgtgggtg caccgttcga
      181 ggcggccagc accgtcccgg tgtcatccac gatctgcact gcaccgatct ggctgtcggt
      241 caccaacaac gacggatcca cctcccgggg cgtatcagtg cgcaattgcg cgctgatctg
      301 ctcggcgcgg gccgcggccg caccctgagc ggtggactcc aaagatcggt agagcaccag
      361 cagcaggaca ccaccggcga tggcaagact gagtgtcatc accaccgcgg cggccagcgc
      421 cgagcgagta cgcacgctcg catgcatcgg gttgagccgg ccgacgatca gaacctcccg
      481 gtgtgcgccc aaggccgcca tgtcatcgca gcgccccgtc gtccctaacc ccaatcatcc
      541 agcacgacgt gatacccatc atcgtggccc gccgacgctg tgaaatcgtt gaaagaacgg
      601 gtttcgttgt ccgccctggt ggcgtcgcag aacaccagca gaggcgtcac cccggttctg
      661 gccggttccg gccgatcggc ggcgctcgtg tccacagaga aatactctca ggctgagggc
      721 agtggagagg cgcgtaatcg gccggatcct ccgtaacgtt gtggtcaccc tcctaaatgt
      781 tgtccgtgtc gcacccccca gcacattcgg cgctgtccgc gagtttcggg gtctccacca
      841 ctgtcggtgc acgcggcgga ggcggcgcgc ccacggcgga ttgagtgagt gttcagcggg
      901 actcagcaag ttctgagcgg tcgtgggcga cgttgtcctc gtagggctct cgtcgcaacc
      961 tcgtggcgaa actaggcaat ttgaaactta ggaagtgcag ccatgtccat gaaggtgaac
     1021 aagtacatca caccgctgct gggcgggact gctgccgcgg tcgcgatggc cgcaccgacg
     1081 gcttccgcag cagatcatca gtcctgcaac gggagcggct cgagaaccgt ctgacagtct
     1141 cccggcaacg ttcagatcac ggcccccgcg ccccaggtca gtttcacccc tacggagacc
     1201 aggccttcct gctcttccac cactgagctg gtgcgtggga cacgggtacc gcatcctcgt
     1261 cgctggcatc gaacttcggc ggcgagggcg cggcgccgtt cgtcgcgcaa ggcatggatg
     1321 tgaaatccgc ccgctgatcc ctattctcga atcgaggttg attcctttgt cagagtggct
     1381 aactcggcaa tgcgctgttc ctcggcgcgt gcgcgagagg actgctctgt gccaagctgc
     1441 cctccaccca ggtacggcca tcggggccaa tacggctgcg atcagccgcg ctcgaccgcg
     1501 gtgctcccat agcggctggc cccggtgcct cgatggttgc gatgttgcgt aggggtgcgc
     1561 ccgttcacgt caccagggcg agggccagcg cgaagtcctg taccgtcaga acgggcatat
     1621 ctccggaggg gtggtgctga aaatcggccg gggaagtctt gatcggaacg ttgccgggcg
     1681 acaggcagac agttcccgag ccactcccgt tgcaggactg ctgatctgtt gcgaacgccg
     1741 tcggtgcggc gacgatcgcg accgcggcag caccggccgc caacagtggt gtgatgtact
     1801 gcagtctcat tcccatggtg gttctccttg aagtgctcga tcatgtgtaa ttgcccgtac
     1861 agaactagcc tcacccacct ccgctgtgaa tccattgagc acagttgaaa atccgttgag
     1921 catccatcaa cacgccatga tctgcagtcg tcccctacct gccggcatgc agaggcgctg
     1981 cgttggacgt gatatggcgc ggtaggggag tactgcctcg atcatcgttc gcctccggtt
     2041 ctgccgggcc cggtgagtcg tgggcgaacg tgattgtcag ctagcaccgt cgcccaaccg
     2101 tccaactcgt gtccgtgtgc tgatctcgtg cattggtgag tcctctctcg ccggatgctt
     2161 caggtgggaa gccgtagcgc agggcagggc cacctcacgg cgggtgatga cagtggtgaa
     2221 accaaggccc agccaccccg gtcgctttag tcgtgggagt gacggccatt agatgctgcg
     2281 ggcgaccgcg gccagctcgg cgctgtgctg gcggatccgc tccgcgctcg ccatcctctg
     2341 ttcccgcgtc gcagcgatct ggttcggtgt cacgaatatc cgtcgccgcc gagcccgcct
     2401 ccacaacgtg gcccggtcgt gatcggagac gccgagtcca tcggttacgt ggtgtcgcct
     2461 gccagggtga tgggacctct ggtttgccat gagcatggga ccgggtcgtt ccggttgtcg
     2521 gccgtgcgtt gatagtggcc gaaggcgtgg ttgccggtca atccgacgta gtcggagggg
     2581 ccggctttcg cgtccctggt ttctggcgtt gccggtagga ctcgccctcg acgacgagtt
     2641 cgtaggctgc tgactgcagt cggtccatcg ccgattgggc cagcagtggg tcggccatca
     2701 tggtcaggat ctcgggtggt tcgcggttgc tggtgatgac ggtcgatgcg gcgcggtggc
     2761 gttcgacgat gatctcgtag aagtcggtcg tctcgttact tccagccggt gcagggcgag
     2821 gcagcgggtc ttgttccacc gcactacgat tcgattctcc ccatagcgaa tgtcgcgcgg
     2881 cgaggtgctc acctgaccct tcaccgccgt ggagaacacc cccacgacgg gcacgccgcc
     2941 gccgtctcac tcgcggtcac cgcctgcacc acaggtgtcc catcggctca gcgctcgaca
     3001 cgctcgacac gctcgcgtgg cagcccaaac aaacagtgtt gtaccgtcgg gcctccaaat
     3061 tgaacgacat acgctatatg agcgaaatca caactgcgcg cttaagattg aacagcccgc
     3121 ttggcggatt tcctgactgt ccagccactc caccgcacga ccaccacgct aagaacgaag
     3181 gctggcaacg tcgtgtggga acaccgggac cttccgatgg gtcgaaagac cccctcttgc
     3241 aacggcgttg aggctacact ctcgtgttca gaagagcgcc tggaccgggc caacgtggac
     3301 cccagctggc cgcctcagaa cggaatcaac ggcaccacga aggtccgcag gacgatcacg
     3361 gccacgaaaa ccgcggtaaa tgccaggtca attgagaagg acccgaaccg cgccggcttc
     3421 aacacccgcc ggaccggggc gatcacgggc tcggtgatgc catgggtgat ccggcgggcc
     3481 tggtagagcc cggtgcggcc gccgaccggg gacagcacgc cgatccagtc gaccaccatg
     3541 cgggcaatca acacgagttc gaacagcaac agaacaattc caagcaagcc tgcgaacacc
     3601 gcacactcct tccgacctcc ccgagtgtcc ggcgtccagc tgagaaggca ctgagttcct
     3661 cgccgctccg ccccgcaggc tgggtgtgtt cggaggtcgt gccgctcgca atctgaccga
     3721 aattgcctct cagcgttcgc tcagcaacgc gtctctatgg tcgcgcgtcg tgagcgaaac
     3781 aacgagttcc gacgccgtca agacctcgcg tgtgctgctg agcaaggtgc ccgaagtcac
     3841 tgtgtggttc tgggtcatca agatcctctg caccacggtg ggtgagagct tcgccgactg
     3901 gatcaacatg actctgggcg tcggcctgac ccagacctcc gtcatcttca cggtcgtcct
     3961 cgccgctgtc ctgggatggc aattgagcct caaccgctat gtgccgttcg tctactggct
     4021 gacggtcgtt ctgctcagcg tgaccggcac gctctacacc gacatcctca cagatgatct
     4081 cggcgttcca ctcgcattga gcacgagcgt cttcgcggtg attctggcca tcgtcttcgg
     4141 cgtatggttc gcccgcgagc ggacgctgtc gatccacagc atcgtcacgc ggccgaggga
     4201 gttgttctac tggctcgcag tcctggtgac cttcgccctg ggcacagcgg ctggcgactg
     4261 gatactggag ctcaccggct ggggacctgg tacgtcggtg ctgctgccgg cgggtctgat
     4321 cgttgcgatc gtgatcgggt ggcggctggg cgcgaacgcg gtgctgtcct tctggctcgc
     4381 ttacattctg acccgcccgc tgggtgcgaa cctcggcgac tggctgggtt tcccgtcgga
     4441 ccaacggggc ctcggcttgg gtgttgccct caccagcgcg atcttcctca ccgcgatcct
     4501 cgcaattgtc ctgtacctga cgcgcacgcg cgccgatgtg atcgaagagt ccgaccaaaa
     4561 ccgcccgcgg accgcaacca agaatgcggc ccgtgagcgg atcatgctcg ggtactacgc
     4621 cgttgtcgcc gccgccacgg gcaccctgct ggtctgggcc gcgggacagc cccaccaaac
     4681 cgcgacggcc agcgagaacg aatccggcgg gtcgtccgtc accgtgacac tcgccccagg
     4741 ggaatcggcg accgctaatt tcccgccggc ggaggtcgcg aagttccgca ccatcgctgc
     4801 ggatacgctc gcacaggtgc gggccggcga ccagtctggg gcgacggccc ggatcaagga
     4861 tctcgaaacc gcgtgggacg acgatcagcc gactctgcag cccatggacg acatcgcctg
     4921 gaccgtcctc gacggccaga tcgacaacgt cctcacggcg ttgcgggcga gcagtcccga
     4981 ccccgcgacc gagacgcaga ctctcagcgc gctactgacc tcgcttaggt gaggcaccgc
     5041 cgaaaacacc catcgcacgg cgattctggt taattgatgt tcgcggtggc gatgcgccgc
     5101 acctcgtccc gtggggacgc tgcagatctg cacgtcgcac tcgctctgtc acctccgtta
     5161 cgtgtggtat ctaaccgctg ttacaattga cctgtccgcg gcgccgagcc tggcgtggca
     5221 cccgcatccg gcggcaagga gtcctttgtg actaacacag ccctgtttct cgccgtgttc
     5281 ctggcgtgcg cggtcgaagc ggtcgaggcg ttgacgatca tccttgcggc gggcacagcg
     5341 cgcgattggc ggtccgccaa taccggcctg ctggccggac tcgtgacgct ggccgtggtc
     5401 gtcgccgccc tcggaccggc gttatccgcg attccgctgg acgggttgcg ggtgttcgtc
     5461 ggcggggtac tgctcgtctt cggcctgcag tggttacgca aggcgatcct gcgcgccagt
     5521 ggtcacaagg cgccccacga cgaggcgatg ttgttcgagg ccactctggc cactgcccgg
     5581 caggccggca ccaagcgtcg cggcatggcg atcgactggt acgccttcac cctgtcgtac
     5641 aagggcgtcg tgctcgaggg cctggaagcg gcgttcatcg ccctgacctt cggggccaat
     5701 cagcacaaca tcccattggc cgccgccgcc gcggtcgccg ctgtgctggt agtggcctct
     5761 gtcggcttcg cggtgcgggc accgctgtcc cgcgttccgg agaacagcat gaagttcatc
     5821 gtcggggtca tgctgatcgc gttcgggatg ttctgggctg ccgagggagc gggtgcgcac
     5881 tggccgctcg acgacgcggc gctgctcgtg ctgattccgg ccgtgacgct gtacgcgctg
     5941 gcattggtgg cgctgttccg gcgacaggcc gccatcgctt cgccggtcaa cgccgacgcg
     6001 gcaatcaccg aggaagcgac acgttcatga tccgcagaat ccgcgcgttc ggcgcgtttt
     6061 ggtacgactt catcatcggc gacgactggg tcgtcgcgct tggcgtagcg ctcgcgttgg
     6121 cgatcacgtt cgcggtgagc cgcagcacgg acgcgccagt gtggtggatc atcgtcgtgg
     6181 ccgtcgcaat tctcttgccc ctcagcgtct tccgcgcgac acgcggccgc cgctgagccg
     6241 cctagccacc ttcatcctgc tcggcatata cgcgtgccgc ataacgctcc aaagcctgtt
     6301 cgcacgcgtc gagcgcctcc acggcggccc cacggttgcg ggtgccgaac acgtcacggg
     6361 ccgcaacctt ggcgtaccag ttcttgagct tcaccagctc gacttcgttc tcctcgagct
     6421 cgccgtacgt gaagtggttg gcgcggtatt ccttgtccag accggcgtga aagtcgcggc
     6481 acttgtccag aatctcgtca tactcgtcgt ccctggcggc ctggaacagc gccaccacgt
     6541 cctgctcgcc ggccactgcc gagcaggaca gcagtacggc cgagccgttc atctccagaa
     6601 tttctcgacg aagtttgcgc aaggcacgtt cggcgccgtc ggtggcaggc aacgccgccg
     6661 ccgaattctg caaatagacc gcgcccagcg atttaagccg ccgccacacg gtcgcgcgga
     6721 gccgggtggg atccgacggg atccgataca ccagcagcaa ccagcctgcc ggatcggggt
     6781 ccgtgtgggc ggaccccgcc ggctcctcgg cgcccctgcc agcatcaccc acagcgtcag
     6841 tctgcccgac atcgcaagcg ctcagctgtt gacgccttgt ggtgcacccg ctattgatgt
     6901 tggcgaccgc accgaagtcg gttggtgaca gcatcggagt gcacccgtcc ggcgcagcgg
     6961 cggttcccat cagccgggtg gggccactac ccggtggccg gattcacccg tcgacctcgg
     7021 tggcgtcgat gatcagcact gtcacatcgt tggggtggtg caatatgcgt cctgattggt
     7081 ttcgcagaca attccggtgt gggtggccac accccagttg atctgcggat cgatcgccga
     7141 gtggctcagt atcgtaagca ctttcactcg gcggccgccc atgtggatcg tgtaccccga
     7201 gggcgcgtgc actggtagac ccggttccat cgcagtgcga tgtccgtcga cctcgatctg
     7261 agaaccgttg gtggaagcgc agtctcgcac ccacagtccg gattcgccga tgtcgaattc
     7321 cagatgcgtc ctcgacatcg ttattgtgtc gtcggcgagt gcgactaggt gttcgacatt
     7381 cttgtccgcc gccgcgaccg ggtagcgtcc gatcagtccc ctgccccgaa ctgcaatcgt
     7441 ggaaccgccg tcgaattcca gccgatactg cgcggggcga ggtttccgcg aatgcgcggt
     7501 gcaccccatg ccgttggtcg cgggacgcgg ggacactcgg aatctgagaa cgttcacagg
     7561 cgcggcttcg gctgtgacgc tcttcagctg cgctcagcga ccccacaatg tcgcgtctgc
     7621 agggtgggag tcgcagccca atctggctgc gtgatgagaa gggaaatgca cgatgctgaa
     7681 tcggaagaac aagatc
//
LOCUS       DASRJA010000415         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1159566,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000415 DASRJA010000000
VERSION     DASRJA010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159566"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFC01_18080"
     CDS             complement(<1..204)
                     /locus_tag="VFC01_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81572.1"
                     /translation="MIPTTGNATSIHRVPRGGRSIVLGVALMLAAQSLHNWRGEPAAE
                     QPPAQQAAAHEAPPDTATPPAPPP"
     gene            complement(210..1817)
                     /locus_tag="VFC01_18085"
     CDS             complement(210..1817)
                     /locus_tag="VFC01_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81573.1"
                     /translation="MVGNGGARALLVIAFEAAFSGGAVVFQDNIVFARVRIRIDGLVD
                     GASYTITHPYGSRVYIAGVDGPNPGTINVTDDVGIGAPMQFDIALHGAIGPFLTPLGF
                     IHPGVPGQLIADGATEVQVTGSPFNTNFFRIQGPSANTIFPANAAGANRAQVNTFVIM
                     GQVASQLGARVDKVTVNKTSTETAVNVWASTAPGQSLVVNIAGSPSLPMQGGGADGQY
                     FARAVFPGGGGGTPASVSVTNLTDFPTTTFPGPSLTDTVEITSAVFTIGGDLLVSARS
                     TDRVGNPTLTASGDGVPPTALNNLGNGEASGSIPMLPGTVPPKVITVESSTGGTAESH
                     VVVEGNGAVGGGVPLVASAGPDQTVASGALVHLSGLNSTGNITTFAWTDNSPLVNLVG
                     ANTATPSFTAPIVGTNLHLDVTLTLLIRDGIGNASVDTMIVHVNGPSGPLDIVTITEA
                     RYVASKNQWRVGGSNTLHQGQSVRIFIGAIGDRSRQLATVPVANNGSWLFQSPNSNGL
                     APLGSDNTVWAESTLPGTAGQLPFRRL"
BASE COUNT          364 a          763 c          926 g          397 t
ORIGIN      
        1 cggcggcggc gccggcggcg tagccgtgtc cggcggtgcc tcgtgcgccg cggcctgctg
       61 cgccggcggc tgctctgccg ccggctcacc gcgccagttg tgcagcgact gcgccgcgag
      121 catcagcgcg accccgagca cgatcgagcg cccaccgcgg ggcacccgat ggatcgaggt
      181 cgcattaccg gttgtcggga tcatggcgat cacaggcgtc ggaagggcag ctggccggcg
      241 gtgcccggca gcgtcgactc ggcccagacc gtgttgtcgg aacccagcgg cgcgaggccg
      301 ttgctgttcg ggctctggaa cagccaggag ccgttgttcg ccacgggcac cgtcgccagc
      361 tggcggctgc ggtcgccgat cgctccgatg aagatgcgaa ccgactggcc ctggtgcagc
      421 gtgttgctgc cgccgacgcg ccactggttc ttgctcgcga cgtagcgggc ctcggtgatc
      481 gtcacgatgt cgagcgggcc gctggggccg ttgacgtgca cgatcatcgt gtcgaccgac
      541 gcgttgccga tgccgtcgcg gatcagcagg gtcagcgtga cgtcgaggtg caggttcgtg
      601 ccgacgatcg gcgcggtgaa ggacggcgtc gccgtgttgg caccgacgag gttcacgagc
      661 ggcgaattgt cggtccacgc gaaggtggtg atgttgccgg tcgagttgag gccgctcaga
      721 tgcaccagcg caccggacgc gaccgtctgg tcgggacccg cgctggcgac gagcgggacg
      781 ccgccgccga ccgcgccgtt gccttcgacg acgacgtgcg actcggcggt gccgccggtc
      841 gacgactcga cggtgatgac cttcggcggc acggtgccgg ggagcatcgg gatgctgccc
      901 gacgcctcgc cgttgccgag gttgttcagc gcggtgggcg ggacgccatc gcccgacgcg
      961 gtcagcgtcg ggttgccgac cctgtcggtg gatctcgccg agaccagcag gtcgccgccg
     1021 atcgtgaaca cggcgctggt gatctcgacc gtgtcggtca gcgacggtcc ggggaacgtc
     1081 gtggtgggga agtcggtcag gttggtgacc gacaccgaag cgggcgtgcc gccgccaccg
     1141 cccgggaaca ccgcgcgcgc gaagtattgg ccatcggcgc cgccgccttg catcggcagg
     1201 ctcgggctgc cggcgatgtt gaccacgagg ctctggccgg gggccgtcga ggcccacaca
     1261 ttgacggcgg tctcggtcga ggtcttgttg accgtcacct tgtcgaccct ggcaccgagc
     1321 tgcgacgcga cctggcccat gatgacgaag gtgttgacct gcgcgcgatt ggcgccggcg
     1381 gcgttggccg ggaagatcgt gttcgcgctc gggccctgga tgcggaagaa gttggtgttg
     1441 aacgggctgc ccgtgacctg gacctcggtc gcgccgtccg cgatcagctg gccggggacg
     1501 ccgggatgga tgaagccgag cggggtcagg aacggaccga tcgcgccgtg cagcgcgatg
     1561 tcgaactgca tcggcgcgcc gatgccgacg tcgtcggtca cgttgatcgt gccggggttc
     1621 gggccgtcga cgccggcgat gtagacacgc gaaccgtacg gatgcgtgat cgtgtagctg
     1681 gcgccgtcga cgagaccgtc gatgcggatg cgcacgcgcg cgaacacgat gttgtcctgg
     1741 aagacgaccg cgccgccgct gaacgcggcc tcgaacgcga tcacgagcag cgcgcgtgcg
     1801 ccgccgttgc cgaccatgct ggcatcggcg ccccaataga acgcttcgtc gggccagttg
     1861 atgttggtgt cggtcggcga cgtcggatag tgcgccggcg agttcgggtc gggcagcagg
     1921 aagccgcagg cgttggggtt gcggcacaag gcgagccgca ggccgttcga gtcctcgtag
     1981 aacagcgggt attggatcgt gggatcgatg ggcccgacgc ggacgagtcc tggaccctgc
     2041 gcgcggatct cattggtgct gccggcaatt gccgtgaggg caagaagacc cgacgcaatg
     2101 gtcgggagta cggatgagcg ctttggtgaa gtgtgagaca tgagtttccc tcggtgagaa
     2161 gggagtgatc tgcccctttg ggcgggatga tatgaggcaa cgaacctggg tcggccgacg
     2221 cgatcgcgtg gaggcgcaat gcggcttgtc gatccccgat acgcgattgt gcctatacca
     2281 tggatccgtc gtgcgaggga cgtgcccccc tcgaaaacaa gcggtcgacg cgcgcgaacg
     2341 ccaatggcca gagcgaacgt ccattggcaa tggacgcttg cgcgcggttc gcggcgatgt
     2401 cccatgcggc tacgcgccga cggatagtgc tctggcgccg cagccggcgg
//
LOCUS       DASRJA010000416         4849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3686454,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000416 DASRJA010000000
VERSION     DASRJA010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3686454"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1830
                     /locus_tag="VFC01_18090"
     CDS             <1..1830
                     /locus_tag="VFC01_18090"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZN81574.1"
                     /translation="AAPTSSAPTTTGFGGGIVVTSNNPSAPAQPGGVLSLTRTLVRNN
                     TATQAAGGIGVFGDNFTSVRAVLWMGESVVSGNVSQGQGGGIHVSRADATIGNSLIIS
                     NTVSGGSLAFGGGLLLGTTAAANITATTIASNVAGLNGGGVFMDDGAALQMSGSRIFG
                     NIANNPNQFGGGGLFVGPNGSSSGTIDSSIIADNTQYQIFENPCPKTRLTYTNNTITP
                     RPGANDLYVSACAPFGTVTSISAFNSLANTSGNNSNLPRFAHFLATPRFGTSFTLAWS
                     AARATSVTISGVGTSGAATGTADVAPSVAVTYSLTAAASAANGGNYGAVTAPVTPVAP
                     PKVAKFVPGDFNGDSVADLALFRPSTGAWYVSGLGQLTWGGGNDIPLSGDFDGDRRAD
                     LAVFRPSTGAWYIRSTASGATFQYTWGGGNDIPVPADYDGDGRTDIAVFRQSTSTWYI
                     YHVGSNTSVSHFWGGPGDVPVQGDYDGDGAADVAIFRPSTGAWYIIQSATGSTRQLVW
                     GGSIDTAVPGDYDGDGRTDIGVFRASTSTWYLTLSATGTTRQVVWGGGGDMAVPGDYD
                     GDGRTDVAIFRPSNGRWYIVHLGTGATASYVWGGAGDLPILRR"
     gene            complement(2616..4067)
                     /locus_tag="VFC01_18095"
     CDS             complement(2616..4067)
                     /locus_tag="VFC01_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81575.1"
                     /translation="MSVDAERFVKTRLTWILGIVCMLFVLLTRTWGLHVDEIRYFHVA
                     IREPLGDALESGKPFVFYVLNYALYHVFRWPAGWLHPLILPVFYAVATVLALWFLAAR
                     AVDAGAHRAWAVAVLLLSPFVLFNASQLMMETAQLPLLSVTLALLLSVSAGRHTSQHI
                     LWLVAAAAGSVLIKETALPALLILILAFFPVLGLRVWPLIAGAAIGSTANALLLRAIH
                     APLPPTHYGGMAQLWTSLTAGQPWARTSSYLGVWGFFVGLAWLGALAVAVRRRDPMSW
                     TLVAAGALSAGGVFLVQLATDPILPFPRYAYPVMWAGLACCGIACARTRTGWVSAAVV
                     VLQLPIAGGLWPGTFSIIEHWPTLITLESYNNSATILSGTPAYGWIATSPRALDDLCV
                     YLPRGNAAGAAHTEMWFRDVARQVRFFDERSYPAFQGCNAARAIVDRRFDVNACQEDV
                     CSPSRYSIRSCLERDVLYFSARIGPTKSRVCLP"
     gene            complement(4105..>4849)
                     /locus_tag="VFC01_18100"
     CDS             complement(4105..>4849)
                     /locus_tag="VFC01_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81576.1"
                     /translation="YVFQGSSQCADVPLAFYMSLAVACVVVNHQLKETDHWIVVAIAG
                     AAGGLAAWTKNEGAVLLVAVAVFLCTWRKESRLKAWLSFAGGAAVPVAAWLWFKLFLS
                     PPNDQFTPGALEGLTARFMDSSRWSMIVDRLTTMIPDWGGVTGGALLMLAIVVVLTYG
                     ADRRSILRGAYALTIVAAMMAGYIFAYAITWKPLAWHIDTSFHRLMTQVWPTLVWAAF
                     QLSGRETVSQSVPDLKALTRPERPTRSPA"
BASE COUNT          952 a         1576 c         1555 g          766 t
ORIGIN      
        1 gccgcgccga cgtcgagcgc accgacgaca accgggttcg gcggcggcat cgtcgtgacc
       61 tcgaacaacc cgagcgcgcc ggcgcagccg gggggcgtcc tcagcctcac ccgcacgctg
      121 gtccgcaaca acacggctac ccaggcggcc ggcggcatcg gcgtgttcgg cgacaacttc
      181 acgtcggtca gggcggtgct ctggatgggc gagtcggtgg tctctggcaa cgtgtcccag
      241 gggcagggtg gtggaattca cgtcagccgg gcggatgcga ccatcggcaa ctccctcatc
      301 atctcgaata ccgtctcggg cgggtcgctc gcgttcggcg gggggctcct gctcgggacc
      361 actgctgccg caaatatcac cgccacgacg atcgcaagca atgtcgccgg cctgaacggc
      421 ggtggggtgt tcatggacga cggggcagcg ctgcagatga gcggatcgcg catcttcggc
      481 aacatcgcca acaatccgaa tcagtttggc ggcggtggcc tgttcgtggg gccgaacggg
      541 agcagcagcg gcacgatcga cagcagcatc atcgccgaca acacgcagta ccagatcttc
      601 gagaatccgt gtccgaagac ccggctgact tacacgaaca atacgatcac accgcggccg
      661 ggggcgaacg atctctacgt ctctgcctgc gcgccgtttg gcacggtcac ctcgatctct
      721 gcgttcaaca gcctcgccaa tacttccggc aacaactcga acctgccgcg gtttgcgcat
      781 tttctggcga cgccgcgatt cggtacgagc ttcacgctgg cgtggagcgc ggcccgagcg
      841 acgtcggtga ccatttccgg cgtcgggacg tcgggcgcgg caaccggcac ggccgatgtg
      901 gcgccgtcgg tggcggtcac ctacagcctg acggcagcgg cctcggctgc caatggcggg
      961 aactacggtg ccgtgaccgc acccgtcaca ccggtggcgc ctccgaaggt ggcgaagttc
     1021 gtgccgggcg atttcaacgg cgatagcgtg gccgacctcg cgctctttcg cccatcgacc
     1081 ggtgcctggt acgtctcggg actgggccag ctgacgtggg gcggcgggaa tgatattccg
     1141 ctgtcgggag acttcgacgg agaccgcagg gcggacctcg ccgtcttccg gccgtcaacg
     1201 ggtgcctggt acatccgatc gacggcttcg ggcgcgacct tccagtacac gtggggcggg
     1261 gggaatgaca ttccggtgcc ggcggactac gacggggatg gacggaccga cattgcggtg
     1321 ttccgccaat ccaccagcac ctggtacatc taccacgtcg gctccaatac gagcgtgtcg
     1381 cacttctggg gcggccccgg cgacgttccc gtgcaggggg attacgacgg agacggcgcc
     1441 gcagacgtgg ccatcttccg gccatccacg ggggcgtggt acatcattca gtcagccacc
     1501 ggatccaccc gtcaactcgt ctggggcgga agtatcgaca cggctgttcc cggggattac
     1561 gacggcgatg gccggaccga catcggagtg tttcgggcat ccactagcac ctggtacctc
     1621 actctgtcgg cgacaggaac cactcgtcaa gttgtctggg gcggtggcgg cgacatggcg
     1681 gtcccgggtg actatgacgg cgacgggcgg accgacgtcg cgattttcag accgtcgaac
     1741 ggccgctggt acatcgttca cttgggcacg ggcgcgacgg cgagctatgt ctggggcggc
     1801 gccggagatc tgccgatcct gagacgctag atgagcagca gccgcgtgac ccgccgtgga
     1861 cggacggtga cctcgaagtc gtctggccgt tgcatcaccg gcactacttg agcgttccgg
     1921 atcctgggag ttgaagatgg ctacggatcc cgtccgcgcc ctcacgtagg tcgggattac
     1981 ttgctcggtc gtatcatcgg ttccgacaaa cccggtacgg ttcaacacaa ggaacgagcg
     2041 agaacgagta ccctcgtcaa agatttggag acgattgaaa cgctccgcaa gtcacttccc
     2101 gactaatggc acgtcgccct gagcgcccca caacagcgtg atggtgccgc ccgccgagta
     2161 gctaatgaac cattgtccag tcgacggccg gaaaatcccg atatctgatc tgccatttcc
     2221 gtcgtagtct ccgggcaccg gtatatcgct accaccgccc cacaacagcg tgatgccccc
     2281 tccccccgag aagctaatga accactcccc ggttgacggc cggaaaatcc cgatatctga
     2341 tctgccattt ccgtcgtagt ctccgggcac cggcatatcg ctaccaccac cccacaacag
     2401 cgtgatgccg tctccccccg agaagcatgc tccggtctcg acagtccgct gcgcttcggt
     2461 cgatgcggcg tcgccggcgg cgcgttgttc atagcgccac agggccgcag cgcagggaat
     2521 gagggcaggg ctgtccgagc cggtcagaat ggcgtccagt ttggccgacg cgtctgcctg
     2581 cagcgtcggg atgactggct atgggtgtct tttcgtcacg gcagacacac acgactcttc
     2641 gtcgggccga tccgagctga aaagtagagc acgtcccgtt cgagacagga ccggatgctg
     2701 tatcgcgacg gcgagcagac gtcttcctga cacgcgttca cgtcgaagcg ccgatccacg
     2761 atggctctcg ccgcgttgca gccctgaaag gccggataag atcgctcatc gaaaaaacgg
     2821 acctgccgtg cgacatcgcg gaaccacatc tcggtatgtg ccgcgccggc cgcgttccct
     2881 ctgggcagat agacgcacaa atcatccaga gcgcgcggag aggtcgcgat ccagccgtac
     2941 gcgggagtgc ctgacaggat cgtcgcggag ttgttgtagg actccagggt aatcagtgtg
     3001 ggccagtgct cgatgatgga gaaggtgcct ggccagagac cgccggcgat ggggagctga
     3061 aggacgacga ctgcggcgct cacccagcca gtccgggtac gcgcgcacgc gatgccgcag
     3121 caggccaacc ccgcccacat taccgggtag gcgtagcggg gaaacggcag aatcgggtcg
     3181 gtggcgagtt gcaccagaaa gacgccacct gccgacaagg ctccggcggc aacgagcgtc
     3241 cacgacatcg ggtcgcgcct tcgcaccgcc accgccaggg cgcctagcca agcgagtccg
     3301 acaaagaacc cccagactcc caagtacgac gaggtccgcg cccatggctg gccagcggtc
     3361 agcgacgtcc acaactgtgc catcccgccg tagtgcgtgg gaggaagcgg cgcatggatc
     3421 gcccgcagga ggagcgcgtt tgccgtgctg ccgatggcag cgcccgcaat gagcggccat
     3481 acacgaaggc caagcaccgg aaaaaacgcg agaatcagaa tcaacagggc ggggagcgcg
     3541 gtttccttga tgagcacgct gccagcggcc gctgccacca gccacaggat gtgctgcgac
     3601 gtgtgccggc cagcggagac cgacaggagc agcgccagcg tcacgctcag gagcggcagc
     3661 tgtgcggtct ccatcatcag ttgtgaggcg ttgaacagga cgaacggcga cagcaggagg
     3721 acggcgacgg cccacgcgcg gtgtgcgccg gcatcgaccg cgcgcgccgc cagaaaccac
     3781 agcgcgagca ccgtcgccac ggcgtaaaag accggaagaa tcagcgggtg caaccatccg
     3841 gccggccatc ggaaaacatg gtagagcgcg tagttgagca catagaacac gaacggtttg
     3901 cccgactcga gggcatcgcc gagcggttcg cgaatggcga cgtgaaagta gcggatctcg
     3961 tcgacgtgca acccccatgt ccgcgtcagt aacacgaaca acatgcacac aatccccaag
     4021 atccaggtca ggcgcgtctt cacgaagcgc tcggcatcga ccgacatgtt agcgcagcac
     4081 gaaaacgggt ctcttccaag tccttcatgc cggcgatctc gttggccgtt ccggacgcgt
     4141 caaagccttc aggtcgggca ccgattgcga cactgtctca cggcccgaca gctggaacgc
     4201 ggcccagacg agcgtcggcc acacttgcgt catcaggcga tgaaacgaag tgtcgatgtg
     4261 ccaggcgagc ggtttccacg tgatggcgta ggcaaaaatg tatcctgcca tcatcgccgc
     4321 gacgatcgtg agagcgtacg cgccgcggag gatggatctc cgatcggcgc cgtaggtcag
     4381 cacgacgacg atcgcgagca taaggagggc gccacccgtc acaccgcccc agtccggaat
     4441 catcgtcgtg agccggtcga cgatcatgga ccaccgtgat gaatccatga accgggcggt
     4501 cagtccctcc agtgccccag gggtgaactg atcgttcggc ggggagagaa acagcttgaa
     4561 ccagagccac gcggccaccg gaacagccgc cccgcccgcg aacgagagcc aggctttcaa
     4621 cctgctctcc ttgcgccacg tgcacaaaaa cacggcgacc gccaccagca ggacggcgcc
     4681 ctcgttcttg gtccaggcgg ccaacccgcc cgccgcgccg gcaatcgcca cgacgatcca
     4741 gtgatccgtc tccttcagct ggtggttcac caccacgcac gccacagcca gcgacatgta
     4801 gaacgcgagg ggcacgtccg cgcactggct ggaaccctgg aatacgtac
//
LOCUS       DASRJA010000417        19081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3991499,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000417 DASRJA010000000
VERSION     DASRJA010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 19081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19081
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3991499"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFC01_18105"
     CDS             complement(<1..217)
                     /locus_tag="VFC01_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016891987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81577.1"
                     /translation="MTGSGSSRLSPRDHRCCRASPSQRRRWPRLNLVGPEQGTASLVA
                     VAIMAVFLAVTAGGVYVGSAVIARHRAQ"
     gene            complement(111..398)
                     /locus_tag="VFC01_18110"
     CDS             complement(111..398)
                     /locus_tag="VFC01_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family type IV pilus minor pilin"
                     /protein_id="HZN81578.1"
                     /translation="MAALVVVLILCVSGLTAVSMQVRCIDAAREAARLAARGDDGSAS
                     GAAQRIAPGGAAVQLRRDGEWVVATVTARSPLLPGITVAAEAVAAAEPGRT"
     gene            complement(440..646)
                     /locus_tag="VFC01_18115"
     CDS             complement(440..646)
                     /locus_tag="VFC01_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HZN81579.1"
                     /translation="MMVANKFRALWARLTLMAVADDGMSTVEYAIGTIAAAAFGAILY
                     TVITGDSIVSALTNIINRALSTNV"
     gene            complement(673..1248)
                     /locus_tag="VFC01_18120"
     CDS             complement(673..1248)
                     /locus_tag="VFC01_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN81580.1"
                     /translation="MSWAALLLAAALLTGVDFRPRVRAGLTSAEPRRWPTPSDTSDPL
                     AAASSFDLFAACLRSGMAVSTAASATAPSAPAPLARVLRRAADLLALGADPATAWTDP
                     NSLADNHADALLRLARRSASSGAALAQGVAELADQSRSDAADAASAAAARASVLIAGP
                     LGLCYLPAFVCLGIVPVVVGLAGDVLESGIL"
     gene            complement(1245..2030)
                     /locus_tag="VFC01_18125"
     CDS             complement(1245..2030)
                     /locus_tag="VFC01_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZN81581.1"
                     /translation="MSFAVLTLAVAILIIPAGSRCRVEGVGLLVPARHRLPVVPCAGI
                     TALALAIVLPVSVVAAFSIIGVTLWLRRRRQVRVRRRAAESAALQGALDVLVGELRVG
                     AHPVAAFDAAAEDVDGAVAESLRAVAARARLGADVAAGLHSVTGTSLLPAHWRRLAVC
                     WQLAQGHGLAIATLMQTAQRDIVERERFSAKVDAGMTGARTTAAVLAGLPLLGIGLGE
                     LIGAHPVRLLVSSGVGAWLLVIGVSLACGGLLWSDRVTARALT"
     gene            complement(2027..3193)
                     /locus_tag="VFC01_18130"
     CDS             complement(2027..3193)
                     /locus_tag="VFC01_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HZN81582.1"
                     /translation="MSASLIDRVRERLAAEPGPLRPNVVAAAIRAESGGVLGDSEVLS
                     SLRVLQTELTGAGILEPLLCADGTTDVLVTAPDAVWIDDGNGLRRSPIQFADEAAVRR
                     LAQRLALSAGRRLDEAQPWVDGQLTGLGTGQFSVRLHAVLPPIAAGGTCLSLRVLRPA
                     TQDLAALTHSGTVAPEAAALLDDIIGARLAFLISGGTGAGKTTLLSAVLGAVAADERI
                     VCVEDAAELAPAHPHLVKLVARSANVEGVGEVAVRDLVRQALRMRPDRIVVGEVRGAE
                     VVDLLAALNTGHDGGAGTVHANNPAEVPARLEALAALGGLDRAALHSQLAAAVQVILH
                     VSRNRTGVRRLSEIGVLQRGDDDRVRVATAWHVDTGFGCGREDLTSLLRSRGRP"
     gene            complement(3190..4260)
                     /gene="ssd"
                     /locus_tag="VFC01_18135"
     CDS             complement(3190..4260)
                     /gene="ssd"
                     /locus_tag="VFC01_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein Ssd"
                     /protein_id="HZN81583.1"
                     /translation="MTATNGILALVGDAALRNDVDRVAAAAGLPVVHASDPSGRSVWT
                     AAVAVVLDVAAARRCAHRALPRRSRVVLVVGSEPHAADFEAAIAVGAQQVIALPARDS
                     ELMAELSDAAEVSRDGDRRGPVVAVIAGRGGAGASVFSTALAQSATGLSEALLVDADP
                     WGGGIDLVLGCEGEAGLRWPDLAVHGGRLSYSALRDALPQRHGVSVLSGSRTGSDVDA
                     APLSAVIEAGSRGGATVICDLPRRSTGAVEAVLDAADLVVIVAPADVRSCAAAAAISP
                     WVSLGNPNAGVVVRGPSPGGLRPTEVAEIVGLPLLAAMRAEPRLADALERGGLRLRRR
                     SALAAAARRVLAALHRRQAVAA"
     gene            4724..5548
                     /locus_tag="VFC01_18140"
     CDS             4724..5548
                     /locus_tag="VFC01_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HZN81584.1"
                     /translation="MAPQTSTPSGKPVRTAAFFDLDKTVIAKSSTLAFSKPFFDQGLI
                     NRRAVLKSTYAQFLFLMSGADHDQMDRMRSYITNMCTGWDVEQVKSIVGETLHDIVDP
                     LVFAEAADLIADHKICGRDVVVVSASGEEIVAPIARALGATHAMATRMVVEDGRYTGE
                     IAFYCYGDGKVEAIRQLAAREGYALEHCYAYSDSITDLPMLAAVGHPTVVNPDRALRK
                     EAAARNWPVVTFSKPVSLRDRIPAPSGAAVATTAAVGVSALAAGALTYSLLRRFAF"
     gene            complement(5962..6702)
                     /locus_tag="VFC01_18145"
     CDS             complement(5962..6702)
                     /locus_tag="VFC01_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZN81585.1"
                     /translation="MTDPLAPLAELPGVAAAGEEAREALGRAHRHRTNLRGWPQTAAE
                     ASLRAARASSVLDGGSLQLTDDGEPDPVLAGALRVAEALEGGATSLVGVWQRAPLQAI
                     ARLHALAAADLADDDRLGRPRGDAEVGRRLALLADIVTGGTNVAAPVLAAVVHGELLT
                     LEPFGTADGVVARAVSRLVTIASGLDPHGLGVPEVYWMRHSGDYLAAARGFSSGTPDG
                     LTAWLVLSARALHGGAREALSIAQAMSS"
     gene            complement(6714..7205)
                     /locus_tag="VFC01_18150"
     CDS             complement(6714..7205)
                     /locus_tag="VFC01_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6653 family protein"
                     /protein_id="HZN81586.1"
                     /translation="MGVMSKVAGAFRMSDDAWKRHANPWSVWTRFAAIPLMILAIWSR
                     VWLGWWCLLPVAAVIAWLWLNPHVFAPVETPTSWAAKGIYGEKLWLQQRDRVPSDHLR
                     VLRMSPACAWAHAPIGVAGFVLLGYGLVRLEVWPTVFGATLVVLGQLWRIDRLVVFYE
                     STQ"
     gene            7329..9275
                     /gene="acs"
                     /locus_tag="VFC01_18155"
     CDS             7329..9275
                     /gene="acs"
                     /locus_tag="VFC01_18155"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZN81587.1"
                     /translation="MSDTHAEVQSVYPPSPEFAAEANAGEELYREAEEDRLAFWAQQA
                     NRLSWDTPFDEVLDWSDAPFAKWFVGGKLNVAYNCVDRHVEAGNGDRVAIRWEGEPGD
                     SRELTYADLLAEVSKAANTLTDLGLVTGDRVAIYMPMLPEAIAAMLACARLGVLHSVV
                     FAGFSSAALAARIEDAEAKLVITSDGQYRRGQAVSLKESVDEAVKGQKSVEHVLVVRR
                     TEIDTPWTDGRDLWWHETVEVASPEHTPEAFDSEHPLFLLYTSGTTGKPKGIMHTSGG
                     YLTQASYTHHNVFDVKPETDVFWCTADIGWVTGHTYIVYGPLSNGVTQVVYEGTPNSP
                     NEHRHFEIIEKYGVTIYYTAPTLIRMFMKWGREFPDAHDLSSLRLLGSVGEPINPEAW
                     RWYRDVFGGNRTPVVDTWWQTETGAIMISPLPGVTEAKPGSAMKPLPGISAKVVDEEG
                     NELVPGADEDEHVTGYLVLDQPWPSMLRGIWGDPERYKETYWARYADQGWYFAGDGAR
                     VDSDGSVWLLGRIDDVMNVSGHRISTTEVESALVGHSGVAEAAVVGASDETTGQGICA
                     FVILEAHAKDNDNMVDVLREQVAKEIGKIARPREIHVVPELPKTRSGKIMRRLLRDVA
                     EGRELGDTSTLVDPSVFEAIRASK"
     gene            complement(9276..9941)
                     /locus_tag="VFC01_18160"
     CDS             complement(9276..9941)
                     /locus_tag="VFC01_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HZN81588.1"
                     /translation="MILALCALLPAPAYADGPVPLGGGSGIVVEGDTFCTLTAIGNDN
                     RGNLIGFTSAHCGGPGERVAAEGAEAAGVLGTMVAGNEALDYAVIQFDPQKVQPVNNV
                     KGFEIDGIGPDPVVGDVACKLGRTTGYSCGVTWGPGEQPGTIVNQVCGQPGDSGAPVT
                     VNNRLVGMIHGAFSEELPTCVIKYIPLHTPAVTMSINAVLGDITAKNRPGSGFVPVGA
                     AAA"
     gene            10109..10621
                     /locus_tag="VFC01_18165"
     CDS             10109..10621
                     /locus_tag="VFC01_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZN81589.1"
                     /translation="MSDRRDGVPTTVTSIPLVDPHAPKADPSIGDLVKDATTQVSTLV
                     RAEVELAKAEITRDVKKGLTGSVYFILALVVLFYSTFFLFFFLAELLDTWLWRWVAFL
                     IVFALMLVVTGVFALLGYLKVRRIRGPQQTIESVKETREALTPGHDKEKALTSRNGEG
                     PGAQTDPSGW"
     gene            10621..11571
                     /locus_tag="VFC01_18170"
     CDS             10621..11571
                     /locus_tag="VFC01_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN81590.1"
                     /translation="MAPPDPSVVRIDGPWRHLEVHANGIRFHVVEAESAEASTTPVTE
                     RPLVILLHGFGSFWWSWRHQLRGLSGARVVAVDLRGYGGSDKPPRGYDGWTLAGDTAG
                     LVRALGHNAATLVGHADGGLVCWATSVLHPRMVQAIAVVSSPHPVALRASALTRRDQG
                     RALLPWMLRYQVPMWPERSLTRNDAEELERLVRSRASAKWLASEDFSETIRHMRQAIQ
                     IPSAAHSALEYQRWAVRSQLRGEGRRFMRSMKRPLAVPVLHLRGDADPYVLADAVYRT
                     QHYAPHGRYVSIAGAGHFGHEEAPEVVNEHLSRFLAQVYR"
     gene            complement(11568..12755)
                     /gene="marP"
                     /locus_tag="VFC01_18175"
     CDS             complement(11568..12755)
                     /gene="marP"
                     /locus_tag="VFC01_18175"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance serine protease MarP"
                     /protein_id="HZN81591.1"
                     /translation="MTSSQWLDLAVLAIAFVAAISGWRSGALGSLLSFVGVVLGAVAG
                     VLLAPHIVTHISGPRTKLFVTLFLILALVVIGEIAGVVLGRAVRGALRNPGLRVIDSV
                     IGVGLQLVAVLVAAWMLATPLTSSDQPNLAAAVRGSKVLAQVDEVAPSWLKSVPTRLS
                     GLLDTSGLPDVLSEYGRTPIIAVDAPNAQLANDAVVAATRPSVVKIRGVAPGCQKVLE
                     GTGFVVAPNRVMSNAHVVAGSDSVTVEVNGDTYDAFVVSYDPEADISILDVPNLPSQP
                     LSFAQGAAPTGTDAIVMGYPGGGDFVATPARVRETIELNGPDIYRTATVTREVYTIRG
                     TVRQGNSGGPMIDRTGKVLGVVFGAAVDDADTGFVLTANEVSRQLAKIGNTEHVATGA
                     CVG"
     gene            complement(12752..13534)
                     /locus_tag="VFC01_18180"
     CDS             complement(12752..13534)
                     /locus_tag="VFC01_18180"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZN81592.1"
                     /translation="MSWGREAGGLTPDAAPPWLKPLTDNCHHVKRAYRRRVPPEVLAL
                     VTAANTKAAITGEKRDAAVLVLFSGPPDSPNGGLPDDADLLVTVRASTLRHHAGQAAF
                     PGGAADPGDDGPVHTALREANEETGIETGRLQPLCTLERMFIPPSGFHVVPVLAYSQD
                     PGPVAVVDESETAIVARVPVRAFINPENRIMVYRKENTRRFAGPAFLLNEMLVWGFTG
                     QVISAMLDVAGWAKPWDTDDVRELDDAMALVGHEDSYGDAQQ"
     gene            complement(13531..14202)
                     /locus_tag="VFC01_18185"
     CDS             complement(13531..14202)
                     /locus_tag="VFC01_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZN81593.1"
                     /translation="MSTSTRWTVAVMVVVVALGVALWTQLRDDGSPTGSVGPGQARDR
                     RDADTAEALAGPRARADLAPCPPAGTLPGPEQLSPDLRSWAHGITLECVGDGTRVDVA
                     KAVAGRPTVLNLWAYWCAPCGEELPAMAEYQRRAGSDVTVLTVHQDENEQAALLRLAE
                     LGVRLPTLQDGRRLVAAALRVPNVMPATVVLRADGSVAEVLPRSFASADEIAAAVDPK
                     IGVPG"
     gene            complement(14217..14897)
                     /gene="nth"
                     /locus_tag="VFC01_18190"
     CDS             complement(14217..14897)
                     /gene="nth"
                     /locus_tag="VFC01_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZN81594.1"
                     /translation="MNRALAQAFPHVYCELDFSNPLELTVATILSAQSTDKRVNLTTP
                     ALFKRYRTAVDYAQADRTELEELIRPTGFYRNKTNSLIRLGQELVERFDGEVPNTMDE
                     LVTLPGVGRKTANVILGNAFDIPGITVDTHFGRLVRRWRWTAEEDPVKVEHAVGELIE
                     RSEWTLLSHRVIFHGRRVCHARKPACGVCVLAKDCPSFGTGPTDPVVAAPLVKGPETE
                     HLLALAGL"
     gene            15149..15442
                     /locus_tag="VFC01_18195"
     CDS             15149..15442
                     /locus_tag="VFC01_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81595.1"
                     /translation="MLATFGLQRLESSLEDDTVSASDVAEFLDQAEPDDVNTLAREGM
                     GPALDGLHQRLSERDWPLDAGTAVRAMASLPNRLVMHHRPNPEFQPTRHANRV"
     gene            15545..16219
                     /gene="crp"
                     /locus_tag="VFC01_18200"
     CDS             15545..16219
                     /gene="crp"
                     /locus_tag="VFC01_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HZN81596.1"
                     /translation="MDEILARAGIFQGVEPSAVAALTKQLQPVDFPRGHTVFAEGEPG
                     DRLFIIISGKVKIGRRSPDGRENLLTIMGPSDMFGELSIFDPGPRTSSATTITEVRAV
                     SMDRDALRAWIADRPEIAEQLLRVLARRLRRTNNNLADLIFTDVPGRVAKQLLQLAQR
                     FGTQEGGALRVTHDLTQEEIAQLVGASRETVNKALADFAHRGWIRLEGKSVLISDSER
                     LARRAR"
     gene            complement(16286..17062)
                     /locus_tag="VFC01_18205"
     CDS             complement(16286..17062)
                     /locus_tag="VFC01_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN81597.1"
                     /translation="MTLTHPAYGLLRPVTETASVLLCNNPGLMTLDGTNTWVLRGPGS
                     DEMVVVDPGPEDDEHIAKVAELGTIPLVLISHRHEDHTGGIDKIVELTGAVVRSVGSG
                     FLRGLGGPLVDGEVVDAAGLRITVMATPGHTADSVSFLLDDAVLTADTVLGRGTTVID
                     NEDGSLGDYMESLRRLHGLGQRTVLPGHGPDLDDLEAVSAMYLTHREERLDQVREALR
                     VLGDDASTRQIVEHVYTDVDEKLWDAAEWSVQAQLDYLRS"
     gene            complement(17059..17514)
                     /locus_tag="VFC01_18210"
     CDS             complement(17059..17514)
                     /locus_tag="VFC01_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN81598.1"
                     /translation="MSASQRLADLGIELPDVVPPAGVYVPAVRTGNLVYTAGQVPMQS
                     GKLAQTGKVGGEVTPEEGKALARICALNALSAIHSLVGIDNVTRVVKVVGFVASAPGF
                     HGQPGVINGASQFLGEVFGDAGAHARSAVGVSELPLDAPVEVELIVEVS"
     gene            complement(17511..17672)
                     /locus_tag="VFC01_18215"
     CDS             complement(17511..17672)
                     /locus_tag="VFC01_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_178004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HZN81599.1"
                     /translation="MSEPTRWEYATVPLLTHATKQILDQWGEDGWELVSVLPGPTGEQ
                     HVAYLKRPK"
     gene            17721..18740
                     /locus_tag="VFC01_18220"
     CDS             17721..18740
                     /locus_tag="VFC01_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HZN81600.1"
                     /translation="MATSANSGRPVGWPSRLTKARLHFVTGKGGTGKSTIAASLGLAL
                     AAGGRKVLLVEVEGRQGIAQLFDVPPLPYAEVKIATADGGGQVNALAIDTEAAFLEYL
                     DMFYNLGLAGRAMRRIGAVEFATTIAPGLRDVLLTGKIREIVTRAEKGKTPVYDAIVV
                     DAPPTGRIARFLDVTKAVSDLAKGGPVHSQAESVVKVLHSDLTAIHLVTLLEALPIQE
                     TLEAIEELQELGLPIGSVIVNRNIPAYLPADDLAKAAEGDIDADTVRTELESVGITLS
                     DSDFAGLLTETIQHATRIKARAESAEQLDELDVARLELPQIADGVDLGSLYELAESLA
                     HQGVR"
     gene            18737..>19081
                     /locus_tag="VFC01_18225"
     CDS             18737..>19081
                     /locus_tag="VFC01_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HZN81601.1"
                     /translation="MSTTPPALDMGAVLTDTSNRVVVCCGAGGVGKTTTAAAMALRAA
                     EYGRTVVVLTIDPAKRLAQALGIKDLGNTPQRVPLAPEVPGELHAMMLDMRRTFDEMV
                     IQYSGRERGEAIL"
BASE COUNT         3311 a         6496 c         6291 g         2983 t
ORIGIN      
        1 cctgagcccg gtgccgcgcg atcaccgcgg acccgacgta cacaccgccg gcagtgactg
       61 ccaaaaacac cgccataatc gcgacggcaa ccaggctggc agtgccctgc tcaggtccga
      121 ccaggttcag ccgcggccac cgcctccgct gcgacggtga tgcccggcaa cagcggtgat
      181 ctcgcggtga cagtcgcgac gacccactcc ccgtcacgcc gtagttgcac agcggccccg
      241 cccggcgcaa tgcgttgtgc tgcaccggaa gccgaaccat cgtcgcctcg tgccgcaagg
      301 cgggccgcct cgcgcgcggc atcgatgcaa cggacctgca tcgacactgc ggtgagcccc
      361 gacacgcaca ggatgagtac gacgacgagc gcagcgatcg cgaacgccgc ctcgaccgtg
      421 acaccgccgg cgtcatcgac tagacgttgg tactcaacgc ccggttgatg atgttggtca
      481 acgcgctgac gatggaatcg ccggtgatca ccgtgtacaa gattgccccg aacgccgctg
      541 cggctatcgt gccaatcgcg tactcgaccg tcgacatgcc gtcgtcggcg acggccatca
      601 gcgtcagccg ggcccacagc gctcgaaatt tgttcgctac catcatgatt tcccctcatc
      661 tcttgtcggc cgtcacaaga ttcctgattc cagtacgtca ccggccaatc cgacgaccac
      721 gggcacgatg cccaaacaca cgaatgccgg cagatagcag aggcccagcg gccctgcgat
      781 caacaccgag gcccgcgcgg cggcggcgct cgccgcgtct gctgcgtcgc tacgcgattg
      841 gtccgccagc tcggcaactc cttgcgcaag tgccgcaccc gatgacgcgg atcgccgggc
      901 gagccgcaac agtgcgtcgg catggttgtc cgccaagctg ttcggatccg tccacgccgt
      961 cgccgggtcg gcgcccaaag ccaacaggtc ggcggcacgg cggagcacgc gcgccagcgg
     1021 ggcaggcgcg gacggcgcgg tcgctgatgc ggccgtcgac accgccattc cggaacgcag
     1081 gcacgcggca aacaggtcga agctggatgc cgcggccaac ggatcactcg tatcggaggg
     1141 cgttggccaa cgccgcggtt cggcagaagt caagccggcc cgaacccgcg ggcggaaatc
     1201 cacaccggtc agcaacgctg cagccaaaag caaagcggcc cagctcatgt cagcgcccgc
     1261 gcggtgaccc gatcagacca aagcaaccca ccgcaggcca gcgagacccc gatgaccagc
     1321 aaccaggctc cgaccccgct cgatacaagt aggcgcacag ggtgtgcacc gatcagctcg
     1381 cccaggccga tgccaagcag cggcaatccg gccagcacag cggcggtcgt gcgtgcgccg
     1441 gtcatgccgg cgtcgacttt ggccgagaag cgctcccgct caacgatgtc gcgctgcgcg
     1501 gtctgcatca gggttgcgat cgccagcccg tgcccctggg caagttgcca acacactgcg
     1561 agccgccgcc aatgtgccgg cagcagcgat gtccccgtca cactgtgcag gcccgcggcc
     1621 acatcggccc ccaagcgcgc acgcgcagcc accgcccgca acgactccgc caccgcgccg
     1681 tccacgtcct ccgcggccgc atcgaatgcc gcgaccgggt gggcccccac ccgcagctcc
     1741 ccgacgagaa catccaatgc cccttgcagc gcggcagatt cggcagctcg gcgtcttacc
     1801 cggacctgcc gacgcctgcg cagccacagg gtgacaccga tgatcgagaa cgccgcgacg
     1861 acactaaccg gcagcacgat cgccagcgcc agcgcggtga tgcccgcgca cggcaccacg
     1921 ggcagccggt gtcgcgccgg cacgagaagg ccaacgcctt cgaccctgca gcgcgatccc
     1981 gcgggaatga tcaggatcgc caccgccaac gtcagcaccg cgaaactcac ggccgacccc
     2041 ggctgcgtag caacgacgtt aaatcctctc tgccacagcc gaatccggtg tcgacgtgcc
     2101 atgcggtggc cactcgcacg cggtcgtcgt ccccgcgttg aagcacaccg atctcgctca
     2161 gcctgcgcac gccggttcgg ttgcggctca cgtgcagtat cacctgcacg gccgccgcga
     2221 gttggctgtg caacgctgcg cggtcgagtc cgcccaacgc ggcaagcgcc tcgaggcgag
     2281 cgggcacctc ggccgggttg ttggcgtgca ccgtcccagc gccgccgtcg tggccggtat
     2341 tgagcgcagc aagcaggtcc accacttcgg cgccccgcac ctcgccgaca acgattcggt
     2401 ccggccgcat ccgcagggcc tgccgcacca gatcccgaac agcgacctcg ccgacgcctt
     2461 ccacgttggc gctccgcgcg accagcttga ccagatgcgg gtgagcgggc gcgagctccg
     2521 cggcgtcctc cacgcaaacg atgcgttcgt cggcggcaac agcgccgagg accgccgaca
     2581 ggagcgttgt cttgcccgcc cctgtgcccc cggagatcag gaatgccagc cgggcgccga
     2641 tgatgtcgtc caacagtgcc gccgcttccg gcgcgacggt gccggaatgc gtcagcgccg
     2701 cgaggtcctg agtggccggc cgcagcaccc gcagcgacaa gcaggttccg cccgcggcaa
     2761 tcggcggcag caccgcatgc agccggacgc tgaactgacc ggtgccgagc cccgtcagct
     2821 ggccgtcgac ccagggctgc gcctcgtcga gtcggcggcc ggccgacagc gccagacgct
     2881 gcgccaggcg tcgcaccgcg gcttcgtcag cgaactgaat tgggctgcgg cgcaatccat
     2941 ttccatcgtc gatccaaacg gcgtcgggcg cggtgacgag cacatcggtg gtgccgtcag
     3001 cgcatagcag cggctcgaga atgcctgcgc cggtcaattc ggtctgcagg acccgcaaac
     3061 tgctcagcac ctcgctgtcg ccgagcacgc caccggattc ggcacggatc gcggcggcca
     3121 ccacattggg tcgcaatggc cccggctcgg cggccaaccg ttcccgcacc cggtcgatca
     3181 acgacgcgct catgcggcga ccgcctgccg ccggtgcagc gctgcgagca cgcgtcgcgc
     3241 agcggccgcc agcgctgatc gccgtcgcaa tcgcaggccg ccacgctcga gcgcatcggc
     3301 caaacgcggc tccgcccgca tcgcggccag cagcggcagt ccgacgatct cggccacctc
     3361 ggtcggtctc aggccgccgg gcgacggccc gcgcacgaca acgccggcgt tgggattgcc
     3421 cagcgacacc cagggcgaga tcgccgcggc agcggcgcac gagcgcacgt cggcgggcgc
     3481 gacgatgacg acgagatcgg cagcgtcgag caccgcctcc accgcgccgg tcgatcgccg
     3541 gggcagatcg cagatcaccg tcgcgccgcc gcggctgccg gcctcgatca cggcactgag
     3601 cggcgcggca tcgacgtcac tgccggttcg actgcccgac aacacgctga ccccatgtcg
     3661 ttgcgggagc gcgtcccgca acgctgagta actgagccga ccgccatgga cggcgagatc
     3721 gggccagcgc agtccggcct cgccctcgca ccccagaacc aggtcgatgc cgccacccca
     3781 cgggtcggcg tcgacgagca gcgcttcaga caaccccgtc gccgattgcg ccaacgcggt
     3841 tgaaaagacc gaggcaccgg ccccgccgcg gcctgcgatt accgcgacga ccgggcctcg
     3901 tcgatcgccg tcgcgggaca cctcggcggc atcggacagt tcggccatca actcgctgtc
     3961 ccgggcgggc agtgcgatga cctgctgtgc gccgaccgcg atggctgcct cgaagtcggc
     4021 ggcgtgtggt tcggaaccca cgaccagaac gacccggctg cgccgcggca gcgctcggtg
     4081 cgcgcatcgt cgggcggcgg ccacgtcgag tacgacggcc actgcggccg tccatacgct
     4141 tcggccggac gggtcggacg catggacgac cggcagcccg gcggccgccg ccacacggtc
     4201 gacgtcgttt cgaagcgcag cgtcgccgac cagcgccaag atcccgttgg ttgcggtcat
     4261 ggcctcagcc tgcaggcggc cgacgacacc tcgccatcac tgcgatggca tctgtggatg
     4321 aatgcgaagc tggggacaaa gctgacaaac cgttaagtat gtctgcactg aaaatcacgt
     4381 tccgcaacca ccgcggacga aagagttgtc cgaaatgcga atacgtcacc ccaaaaaagg
     4441 gacgaccccc gccagggggg gaggaggcgg aggtcgtcgt gtatcagccc cggggggtcg
     4501 ggctgatgca cacccggcat aagccgagta atgctcacta tacacacggg actgcggatg
     4561 ggcgcaagtg ctcgttgcca gggaaagcac tcgggagcag gaaagattgc agtcaccggc
     4621 acctgtcgtg aactgccaat ttgtacactc tgtcgccatt tgtcacaccc cggcacctat
     4681 gcttggcgct gtgaccgcat ccgaacccgc ggccagcggg tccatggcgc cgcaaacatc
     4741 gactccatcc ggcaaacccg ttcggaccgc ggctttcttc gatcttgata agactgtcat
     4801 cgccaagtcg agcacattgg ccttcagcaa acctttcttc gatcaagggc taatcaatag
     4861 gcgtgcggtt ctcaagtcga cctacgcgca gtttctgttc ctgatgtcgg gtgccgatca
     4921 cgaccaaatg gaccggatgc ggtcgtacat cacgaacatg tgcaccgggt gggacgtcga
     4981 gcaggtgaag tcgattgtcg gtgagacgct gcacgacatc gtcgacccac tggtcttcgc
     5041 ggaagccgct gacttgatcg ccgaccacaa gatctgcggg cgcgatgtgg tggtggtttc
     5101 ggcctctggc gaggagatcg tcgcgccgat cgcgcgtgcc ctgggagcca cccacgcgat
     5161 ggccaccagg atggtcgtcg aggacggccg ctacaccggc gagatcgcct tctactgcta
     5221 cggcgacgga aaagtcgagg cgattcgtca gctcgccgca cgcgagggct acgcgctcga
     5281 acattgctat gcctactccg actccatcac cgacttgccg atgctcgcgg ccgtcggcca
     5341 tcccaccgtg gtgaacccgg atcgcgcatt gcgcaaagaa gcggcggcgc ggaactggcc
     5401 ggtggtcacc ttctccaagc cggtgtcgct acgggaccgc attccagccc catcgggcgc
     5461 cgcggtcgca accaccgcgg cggtcggagt gagcgcgctg gccgcgggcg cgttgaccta
     5521 ttcgctgctg cgccggttcg ctttctgaag cgctacttcg tgtagtagct agcgcccgcg
     5581 taacgatcag gattcgcaca gaatttcgcc cttgatgtga cgggagtcac ggagtacaaa
     5641 ggaagcacgg aagcctggtt aggccaaggc cgagccggaa gagaaggctc gatctcccga
     5701 agtcaggcta cccagcacgg atcccggcac ccacgcggag cacatgccgc ggaataggca
     5761 aaagtgttgc gggcctgcgt aattgcgaag agcgaacggc cacgacggcc ctttgggtgg
     5821 ggttgcagtc gaagcgcatc gcaaggacgc cgaggtcacc cacgcaaccc acacagcacg
     5881 cttggtaacc ggagtccgtg ctagcgggcg gcgagccgaa tcagttcgga gcgtcgcccg
     5941 cttcgccgtt gctggggcac tttaggacga catcgcctgc gcgatcgaca acgcctcgcg
     6001 cgcaccgccg tgtaatgccc gggcgctcaa cacaagccac gctgtgaggc cgtccggcgt
     6061 acccgacgaa aacccgcgcg cggcagccag gtagtcgccc gagtgccgca tccaatacac
     6121 ctccggcacg ccgaggccgt gtgggtccag accgctcgcg atggtcacca aacgggagac
     6181 cgcgcgagcc accacaccgt cggcggtacc gaacggctcc agcgtcagca gctcgccgtg
     6241 cacgaccgcg gcgagcaccg gcgcagccac gttcgtcccg ccggtgacga tgtcggccag
     6301 cagtgccagc cggcggccga cctccgcgtc acctcgtggc cggcccagcc ggtcgtcgtc
     6361 ggccaggtct gctgccgcca atgcgtgcaa tcgagcgatg gcctgaagcg gggcccgctg
     6421 ccagacaccc accaacgacg tggccccacc ttccaaggcc tcggccaccc gcagcgcccc
     6481 cgccagcacc gggtctggtt cgccgtcatc cgtcagttgc aaggacccgc cgtcgagaac
     6541 cgacgacgcg cgcgcggccc gcagagaggc ctcggccgcg gtctgtggcc atccgcgcag
     6601 attggtgcgg tggcggtgcg cacggcccag cgcctcgcgg gcttcctcac cggccgcggc
     6661 cacacccggc aactcagcca gcggcgccag cggatcggtc acggcagcac aggctactgc
     6721 gtgctctcgt agaacacgac cagccggtca atccgccaca gttggccgag cacgacaagg
     6781 gtggcgccga acacggtcgg ccacacctcg agcctgacga gcccatagcc cagaaggaca
     6841 aagccggcga cgccgatcgg ggcgtgggcc cacgcgcaag ctggggacat gcgaagcacc
     6901 cgcagatgat cggatggcac gcgatcacgc tgctgcagcc acagcttctc accgtagatg
     6961 cccttggccg cccagctcgt cggagtttcc accggcgcga agacgtgcgg gttcagccag
     7021 agccaggcga tcaccgcggc gaccggcagc aggcaccacc agcccagcca tacccggctc
     7081 cagatcgcca ggatcatcag cgggatcgct gcgaaccggg tccacacgct ccacggattg
     7141 gcgtgccgct tccacgcgtc atcactcatc cggaacgccc ctgccacctt cgacatgacg
     7201 cccatgcgga cattgtccaa ccgctggacc catcggcaat gacgttcgtc gcgccgcaca
     7261 tgccgcccgc agaatgcggt tgtgtcatgc caacctcgat gcaacgttcg gtgactacgc
     7321 tcacagacat gtccgacacg cacgccgaag ttcagtccgt ctaccctcca tcgcctgaat
     7381 tcgccgcgga ggccaacgca ggtgaggagc tgtaccgcga ggccgaagag gatcggctgg
     7441 cgttttgggc gcagcaggcc aaccggttgt cctgggacac accgttcgac gaggtactgg
     7501 attggtcgga tgccccgttc gcgaaatggt tcgtcggcgg caagctcaac gtcgcctaca
     7561 actgcgtcga ccggcatgtc gaggcgggca acggcgatcg ggtggcgatc cgctgggagg
     7621 gcgaacccgg tgacagccgc gagctgacgt acgcggatct gctggccgag gtgagcaagg
     7681 ccgccaatac gctcaccgat cttggactgg tgacgggcga tcgggtggcg atctacatgc
     7741 cgatgctgcc ggaggcaatc gcggcgatgc tggcgtgcgc acggctcggc gtgctgcatt
     7801 ctgtggtgtt cgccggtttc tcctcggccg ccttggccgc tcgcatcgag gatgccgagg
     7861 ccaagctggt gatcacgtcc gatggccagt accgccgcgg acaggcggtg tcgctgaagg
     7921 aatcggtcga cgaagccgtg aaaggacaga agtcggtcga gcacgttctg gtggtgcgcc
     7981 gcaccgaaat tgacacaccg tggaccgacg gtcgagacct gtggtggcat gagacggtcg
     8041 aggtggcgtc gcccgagcac acacccgagg cgttcgactc cgaacatccg ttgttcctgt
     8101 tgtacacgtc gggaaccacc ggcaagccca agggcatcat gcacacctct ggtgggtacc
     8161 tgacgcaggc gtcctatacc caccacaatg tgttcgacgt caagccggag accgacgtgt
     8221 tttggtgcac agccgatatc ggctgggtga ccggacacac ctacatcgtc tacgggccgc
     8281 tgtccaacgg cgtcactcag gtggtctacg agggcacacc gaactcgccg aacgagcatc
     8341 ggcacttcga gatcatcgaa aagtacggcg tcaccatcta ttacaccgcg ccgacgttga
     8401 tccggatgtt catgaagtgg ggccgcgagt tccccgatgc ccatgacctg tccagcctgc
     8461 ggctgctggg aagcgtgggt gagccgatca acccggaggc gtggcgctgg taccgggatg
     8521 tgttcggcgg caaccgaact ccggtcgtcg acacgtggtg gcagaccgaa accggcgcga
     8581 tcatgatctc accgctgcca ggtgtcaccg aggccaagcc ggggtcggcg atgaagccgc
     8641 tgccgggcat ctcggcgaag gtcgtcgacg aagagggcaa cgaactcgtg cccggcgccg
     8701 acgaggacga gcacgtgacc ggctacttgg tgctggatca accgtggccg tcgatgctgc
     8761 gcggcatctg gggcgacccg gagcgttaca aggagacgta ctgggcgcga tacgccgacc
     8821 agggttggta tttcgccggc gacggggcac gcgtcgactc cgacggatcg gtttggctgc
     8881 tgggccgtat cgacgacgtg atgaacgtgt cgggccaccg gatctcgacg acggaagtgg
     8941 aatccgcgtt ggtcgggcac tccggggtgg ccgaggccgc ggtggtgggc gccagcgacg
     9001 agaccaccgg tcagggcatc tgcgcgttcg tcattctcga ggcgcacgcc aaggacaacg
     9061 acaacatggt cgacgtgcta cgcgagcagg tcgccaagga gatcggaaag atcgcgcggc
     9121 cacgcgagat tcacgtcgtg cccgaacttc cgaagacgcg cagcggcaag atcatgcggc
     9181 ggctgctgcg tgacgtcgcc gaaggccgcg aactcggcga cacgtcaacc ttggtggacc
     9241 ccagcgtgtt tgaggcaatc cgggccagca agtagtcagg ccgcagcggc accgaccggg
     9301 acgaagcctg aacccggccg gttcttggcg gtgatgtccc caagcacggc gttgatcgac
     9361 atggtcaccg ccggagtgtg cagtgggatg tatttgatga cgcacgtggg caattcctcg
     9421 gagaacgccc cgtggatcat gccgaccagt cggttgttga cggtgacggg tgcgccggaa
     9481 tcgccgggct gtccgcagac ctggttgaca atggtgccgg gttgctcgcc cggcccccat
     9541 gtgacgccgc acgaataacc cgtcgtccga ccgagtttgc aggcaacgtc gccgacgacg
     9601 ggatcgggac cgatgccgtc gatctcaaac cccttgacgt tgttgaccgg ttgcaccttc
     9661 tgcgggtcga attggatcac cgcgtagtcg agtgcttcat taccggcgac catcgtgccg
     9721 agcacaccgg cagcctcggc accttcggcg gctacccgtt cgcccggccc gccgcagtgc
     9781 gcggaggtga agccgatgag attgccgcgg ttgtcgttgc cgatcgcggt gagcgtgcag
     9841 aaggtgtcgc cctcgacgac gatgcccgaa ccgccgccca aaggaaccgg cccgtcggcg
     9901 tacgcgggag ctggtagcag cgcgcacagc gccaggatca ccgcggaaat cgtcaggcag
     9961 cggacgctgg tcctcaactt ctcaaccccc atcgacgtgc cgcctgacca tccaaccggg
    10021 ccagtctatc gtcggcccgg atcgtatccg tggacgccac gtggcaacat gaacgcgact
    10081 gacaccgcag gcggggagga agtgttccgt gagtgatcgc agggatggcg taccgacgac
    10141 ggtgacctcg ataccgctgg tcgacccgca tgcgcccaag gccgatccgt ccattggtga
    10201 cctcgtcaaa gacgcgacga cccaggtgtc gacgctggtg cgcgccgagg tcgaattggc
    10261 caaggccgag atcacccgcg acgtcaagaa gggcctcacc ggcagcgtgt atttcatcct
    10321 cgcgctggtg gtgctgtttt actccacgtt ctttctgttc ttcttcctcg ccgagctgct
    10381 cgatacctgg ctgtggcggt gggtggcctt cctgatcgtg ttcgccctga tgctcgtcgt
    10441 cacgggagtg ttcgcactgc tcggctatct caaggtgcgc cgtatccgcg gaccgcagca
    10501 gaccatcgag tcggtgaagg aaacccgcga ggcgctcacg cccgggcatg acaaggagaa
    10561 ggcgcttacc tcccggaacg gtgaggggcc cggcgcgcag accgacccgt caggctggta
    10621 atggccccac ccgacccgtc ggtggtgcgc atcgacggcc cctggcgcca tcttgaggtg
    10681 cacgccaacg ggattcgctt tcatgtcgtg gaggcggagt cggcagaagc atccacaacc
    10741 ccggtcaccg aacgcccgct ggtgatcctg ctgcacggct tcggctcgtt ctggtggtcc
    10801 tggcgccacc agttgcgtgg cttatccggt gccagggtgg tcgcggtcga cctgcgcggc
    10861 tatggcggca gcgacaaacc tccccgcggc tacgacggtt ggacgcttgc gggtgacacc
    10921 gctggtctgg tgcgcgcgct cgggcacaac gccgcgacgc tggtgggcca cgccgacggt
    10981 ggcctggtgt gttgggcgac ctctgtgctg catccccgaa tggtgcaggc gatcgccgtg
    11041 gtcagctcgc cgcatcccgt cgcactgcgg gcctctgccc tgacgcggcg tgaccagggc
    11101 cgggcgctgc tgccgtggat gctgcgctat caggtgccga tgtggccaga gcggtcgctg
    11161 acccgcaacg acgccgagga gctggagcgg ctggtgcgca gccgagccag cgccaaatgg
    11221 cttgcctcag aagacttttc cgagacaatt cggcatatgc ggcaggccat ccagattccg
    11281 tcggccgcgc attcggcact ggaatatcag cgctgggcgg tgcgcagcca gctgcgcggc
    11341 gagggcaggc ggttcatgcg ctcgatgaag cggccgctgg cggtgccggt gctacacctg
    11401 cgcggtgacg ccgacccgta cgtgctggcc gatgcggtgt accgcacaca gcactacgcc
    11461 ccgcacggtc gttacgtatc cattgccggc gcagggcatt tcggccacga ggaagccccc
    11521 gaggtggtca acgagcatct gtcgcggttc ctggcgcagg tgtaccgcta gccgacgcag
    11581 gcgcccgtcg cgacgtgttc cgtgttgccg atcttggcga gctggcggga tacttcgttc
    11641 gcggtcagca cgaagccggt gtcggcgtcg tcgacggcgg cgccgaacac cacgccgagc
    11701 accttgcccg tccggtcgat catcggacca cccgaattac cttgccgcac agtgcctctg
    11761 atcgtgtaca cctcgcgggt cactgtggcg gtgcgataga tgtcgggacc gttgagctcg
    11821 atggtctcgc gcaccctcgc gggcgtggcg acaaaatcgc cgccaccggg atagcccatc
    11881 acgatggcgt cggtaccggt cggcgcggcg ccctgggcga acgaaagggg ctgcgacggc
    11941 aggttcggca cgtcgagaat cgagatgtcg gcttccgggt catacgacac cacgaacgca
    12001 tcgtaggtgt caccgttgac ctcgaccgtg acgctgtcgg agcccgcgac cacgtgcgcg
    12061 ttggacatca cccggttcgg tgcgacaacg aaaccggtgc cttccaagac cttttggcac
    12121 ccgggtgcga caccgcggat cttgacgacg ctgggccggg tggcggccac gacggcgtcg
    12181 ttggccagct gggcattcgg cgcgtcgacc gcgatgatcg gggtacggcc gtattccgag
    12241 agcacgtcgg gcaggccgga cgtgtcgagc agacccgaca ggcgcgtcgg gacagatttc
    12301 agccagctcg gtgcgacctc gtccacctga gccagcacct tcgagccgcg cacggcggcg
    12361 gccagattgg gctggtccga cgacgtcagc ggggtggcca acatccacgc cgccaccagc
    12421 accgcgacca gctgcaggcc aacgccgatc acggagtcga tcacgcgcag accggggttg
    12481 cgcagagcgc cccgtaccgc gcggcccagc accacgccgg cgatctcacc gatcaccacc
    12541 agcgcgagga tcaggaacag ggtgacgaac agcttggtgc gggggccgct gatgtgcgtg
    12601 acgatgtgcg gggccaacag cacacccgcg accgcgccga ggaccacccc gacgaaggac
    12661 agcagggagc cgagcgcccc ggagcgccat ccggaaatgg cagcaacgaa cgcgatcgcg
    12721 aggacggcaa ggtcaagcca ctgtgacgat gtcattgttg agcatcaccg tagctgtcct
    12781 cgtgcccgac gagcgccatt gcgtcgtcga gttcccggac gtcatcggtg tcccacggct
    12841 tggcccagcc cgccacatcg agcatcgccg agatcacctg accggtgaac ccccacacca
    12901 gcatctcgtt gagcaggaac gccgggccgg cgaagcggcg cgtgttttcc ttgcggtaca
    12961 ccatgattcg gttctcgggg ttgatgaacg cgcgcaccgg gacccgagcg acgatcgcgg
    13021 tctccgactc gtcgacgacg gcgaccggtc cgggatcctg tgaatacgcc agcacaggta
    13081 cgacatggaa cccggacggc gggatgaaca tccgctccag tgtgcacagc ggctgcagcc
    13141 ggcccgtctc gatgccggtc tcctcgttgg cttcgcgcag cgcggtgtgc accggtccgt
    13201 cgtcgcccgg gtcggccgcc ccacccggga atgcggcctg gccggcgtga tggcgcagcg
    13261 tggaagcccg cacggtgacc agcaggtcgg cgtcgtcggg cagaccgccg ttgggcgagt
    13321 ccggcgggcc ggagaacagc acgagcaccg cggcgtcgcg cttttctcca gtgatcgcgg
    13381 ccttggtgtt ggccgccgtc accagcgcca gcacttcggg gggcacgcgc ctgcggtagg
    13441 cccgctttac gtggtggcag ttgtcggtta gcggcttgag ccacggcgga gcggcgtcag
    13501 gggtcagccc gcccgcttca cggccccagc tcaccccggc actcctatct ttggatccac
    13561 tgccgcggcg atctcgtcgg cgctggcgaa ggacctcggc aggacttcgg caacgctacc
    13621 gtccgcgcgc agcaccaccg tcgcgggcat gacgtttggc acccgcaacg cggcggcgac
    13681 cagcctgcgc ccgtcctgca gcgtcggcag gcgcacgccg agttcggcca gccgcagcaa
    13741 tgctgcttgt tcgttctcgt cctggtgcac ggtcagcacg gtgacgtcgg atccggcccg
    13801 gcgttgatac tcggccatcg cgggcagttc ctcgccgcat ggcgcgcacc agtaggccca
    13861 caggttgagg accgtcggtc gccctgcgac ggcctttgcg acgtccacgc gtgtgccatc
    13921 accgacgcat tcaagggtga tcccgtgcgc ccacgaccga aggtcagggg acaactgctc
    13981 gggtcccggc agggtccctg cgggcggaca cggggcaagg tcggcccggg cccgcgggcc
    14041 tgccagcgcc tcggcggtgt cggcgtcacg gcgatcgcgg gcctggcccg gcccgacaga
    14101 cccggtcggc gacccgtcgt cccgcagttg cgtccacaac gcgaccccga gcgcgacgac
    14161 gacgaccatc accgcgacgg tccatcgggt cgacgtgctc atgtgggcag gctgggtcag
    14221 aggccggcca gcgccagcag gtgctcggtc tcgggcccct tcaccagcgg ggcggcgacg
    14281 accggatcgg tcggcccggt gccgaacgac gggcagtcct tggccaagac gcagacgccg
    14341 caggcgggtt tccgcgcgtg gcacacccgg cggccgtgaa agatcacccg gtgggacagc
    14401 agcgtccact cgctgcgctc gatgagttca ccgaccgcgt gctcgacctt taccgggtcc
    14461 tcctctgcgg tccaccgcca ccgtcgcacc agccggccga aatgggtgtc gacagtgatg
    14521 ccggggatgt cgaacgcgtt gccgaggatg acgttggcgg tcttgcggcc gacacctggc
    14581 aatgtcacca actcgtccat ggtgttgggc acctcgccgt cgaaccgctc cacgagttcc
    14641 tggccgaggc ggatcagcga gttggtcttg ttgcggtaga aaccggtggg ccggatcagc
    14701 tcttcgagtt cggtgcggtc ggcttgcgcg taatcgaccg ccgtgcggta gcgcttgaac
    14761 agggcaggcg tggtgaggtt gacgcgcttg tcggtgctct gtgccgacag aatggtggcg
    14821 accgtcagct ccagcggatt gctgaagtcc aactcgcagt aaacatgcgg aaatgcttgt
    14881 gccagtgcgc gattcatcct gcgggcgcgg cggaccagcc cgacgcgggt ttcgttgtcc
    14941 cacttccgcg gggaggcgcg ccgagccggc ttggcggctg ttgtgttatc ggctgaagtc
    15001 tcacccacgg tcacggatgc cagcctactg acgggctctg actttcctgg agcggaccgg
    15061 tgacaattcg taatcccgct gagaccttgg ccgtgtttac ttcacccttg tgtcttggtt
    15121 gctcgctgcg tttattccgg cgttgctgat gttggcaaca ttcgggctgc agcggctcga
    15181 atcgagcctc gaggacgaca ccgtctcggc ttccgatgta gccgaattcc tcgaccaagc
    15241 cgaacccgat gacgtgaaca cgctggcgcg agaaggcatg ggccccgcat tggatggcct
    15301 gcatcagcgg ttgagcgaac gggattggcc gctggatgcc ggaacagctg tccgtgcgat
    15361 ggcaagtttg ccaaatcgcc tggtcatgca tcaccggccg aatcctgaat ttcagccgac
    15421 tcgacatgcc aatcgtgtgt agcgtgggct agtcgcgaaa tggcctacct ctagactgat
    15481 ctcgctaacc agtaagaggt cggcttgcgc atgtcatatc acccaacagc ttaagagggg
    15541 caacgtggac gagatcctgg cgagggccgg aatcttccag ggggtcgaac ccagcgccgt
    15601 agccgcgctg acgaagcaat tgcagcccgt cgacttccca cgcgggcaca ccgtgttcgc
    15661 ggagggagag cctggcgacc ggctgttcat catcatttcg gggaaagtga agatcggccg
    15721 ccgctcgccg gacggtcgcg agaatctgct caccatcatg ggcccgtcgg acatgttcgg
    15781 tgaactgtcg atcttcgacc ctggcccgcg gacgtcgagc gccacgacca tcaccgaggt
    15841 gcgggcggtg tcaatggacc gcgacgcgct gcgcgcgtgg atcgccgacc gccctgaaat
    15901 cgctgagcaa ttgctgcgag tcctggcccg ccggctccgt cgcaccaaca acaatctggc
    15961 cgatctgatc ttcaccgacg tgcccggccg ggtggccaag cagctgctgc aattggccca
    16021 gcggttcggc acacaggagg gcggcgcgct gcgcgtgacg cacgacctga ctcaggagga
    16081 gatcgcgcag ctggtcggcg cgtctcgtga gacggtcaac aaggcgcttg ccgatttcgc
    16141 ccaccgtggc tggattcgct tggagggcaa gagcgtgttg atctcggact cggaacgact
    16201 ggcccgtcgc gcgcgatagc gatttcggtg cgctaataga cgctgagcgc aagacgctga
    16261 gcgcacgtaa gcgcaccgaa atcactcaac tacgcaggta gtccaactgc gcttggacgc
    16321 tccattcggc tgcgtcccat agcttttcgt cgacgtcggt gtagacgtgc tcgacgatct
    16381 gtcgcgtact ggcgtcgtcg ccgagcacac gcaatgcctc gcgtacttgg tcgagtcgct
    16441 cttcgcggtg cgtcaggtac atggcgctga cggcctcgag gtcgtcgagg tcggggccgt
    16501 ggccgggcag cacggttcgt tgcccgagtc cgtgcaggcg gcgcagcgac tccatgtagt
    16561 cgccgaggct gccgtcttcg ttgtcgatca cggtggtgcc gcgcccgagc acggtatcgg
    16621 cggtcagcac cgcgtcgtcg agcagaaacg acaccgagtc ggcggtgtgg ccgggcgtgg
    16681 ccatcaccgt gatgcgcagg cccgcggcgt cgaccacctc gccatcgacc agcggaccgc
    16741 caagcccacg caggaacccg ctgccgacag agcggaccac tgcgccggtt agttcgacga
    16801 tcttgtcgat gccgccggtg tggtcctcat gcctgtggct gatcaacacc agcgggatcg
    16861 tgcccagttc ggcgaccttc gcgatgtgct cgtcgtcctc cggcccgggg tccacgacca
    16921 ccatctcgtc gctgccggga ccgcgcagca cccaggtgtt ggtgccgtcg agcgtcatca
    16981 gccccgggtt gttgcacagc aacaccgacg cggtttcggt gacgggtcgc agcagcccgt
    17041 acgcggggtg cgtaagggtc acgaaacctc gacgatgagc tcgacttcca ccggtgcgtc
    17101 gagcggcagc tccgagacac cgaccgcaga gcgagcgtgc gcgcctgcat cgccgaacac
    17161 ctcaccgagg aactgcgaag ccccgttgat cacgcccggt tggccgtgga atcccggcgc
    17221 cgatgcgaca aacccgacga ccttgaccac ccgcgtgacg ttgtcgatgc caaccagcga
    17281 atggatcgcg gacaacgcgt tgagtgcgca gatgcgcgcc agcgccttgc cctcctccgg
    17341 agttacctcg ccgcccacct tgccggtctg ggccagcttg ccggactgca tcggcacctg
    17401 acccgcggtg tagaccaagt ttccggtgcg caccgcgggc acatagacgc ccgccggcgg
    17461 gacgacgtcg ggcagctcga tgcccaggtc ggcgagccgc tgcgaggcac tcatttcggg
    17521 cgcttcagat acgcgacgtg ctgctcgccc gtcggtcccg gcagcaccga caccagttcc
    17581 caaccatcct cgccccactg gtcgaggatc tgtttcgtcg catgcgtcaa cagcgggacc
    17641 gtggcgtatt cccaacgggt cggttcgctc atggggctga gcttatcggg cggcattcac
    17701 agcttggtta gcatgcatgg gtggctacct cagcgaacag cggtagaccg gtcggctggc
    17761 cgtcgcgcct gaccaaggcg cgtctgcatt tcgtcaccgg caagggcgga accggaaagt
    17821 cgaccatcgc ggcctctctg gggttggcgc tggccgccgg tggccgcaag gtgttgttgg
    17881 tggaagtcga agggcgccaa ggtattgcgc agctcttcga tgtgccgccg ctgccctatg
    17941 cggaggtcaa gatcgccacc gccgacggtg gcggtcaggt caacgcgctg gccatcgaca
    18001 ccgaggcggc gttcctggaa tacctcgaca tgttctacaa cctcggcctg gccggccgcg
    18061 cgatgcgccg gatcggcgcg gtcgagttcg cgacgacgat cgcaccgggc ctgcgtgacg
    18121 tgctgctcac cggcaagatc cgcgagatcg tgacccgcgc cgagaagggc aagacaccgg
    18181 tctacgacgc aatcgtggtg gatgccccgc cgacgggccg catcgcgcgg ttcctcgacg
    18241 tcaccaaggc ggtgtcggat ctggcgaaag gtggcccggt gcactcgcag gccgaaagcg
    18301 tggtcaaggt cctgcattcg gatctcactg ccattcacct cgtcacgctg ctggaggcgc
    18361 tgccgatcca ggagacgctc gaagccatcg aggaactcca ggaattgggc ctgccgatcg
    18421 gcagcgtgat cgtcaaccgc aacatcccgg cctatctgcc tgccgatgac ctcgccaagg
    18481 ccgcggaagg cgacatcgac gccgacaccg tccgcacgga gctggagagc gtcggaatca
    18541 ccttgtccga cagcgacttt gccgggctgc tgaccgagac gatccagcac gcgacgcgta
    18601 tcaaggcgcg tgcggagagc gccgagcagc tcgatgagct cgacgttgcg cggctggagc
    18661 tgccgcagat agcagacggc gtcgacctcg gcagcctcta cgagctcgcc gaatcgcttg
    18721 cccaccaggg ggtcagatga gtacgacgcc gcccgccctt gacatgggcg cggtcctcac
    18781 cgacacgtcc aatcgcgtcg tggtgtgttg cggcgccggg ggtgtcggca agaccaccac
    18841 cgcggcggca atggcgttgc gcgcggccga atatggccgc accgtggtcg tgctgaccat
    18901 tgaccccgcc aagcggctgg cccaggcgct cggcatcaag gatctcggca acactccgca
    18961 acgggttccc ctcgccccgg aggtgccggg cgaactgcac gcgatgatgc tcgacatgcg
    19021 ccgcaccttc gacgaaatgg tgatccagta ctcgggacgc gaacggggcg aagcgattct
    19081 g
//
LOCUS       DASRJA010000418         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4987, whole
            genome shotgun sequence.
ACCESSION   DASRJA010000418 DASRJA010000000
VERSION     DASRJA010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4987"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFC01_18230"
     CDS             <1..220
                     /locus_tag="VFC01_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HZN81602.1"
                     /translation="ARRYGPGWLSSNQLALVNGQLGSYTVESTPTDGYPAVMPRVTAM
                     AVRDGKVCAIWDIANPDKFTGSPLATPR"
     gene            complement(189..803)
                     /locus_tag="VFC01_18235"
                     /pseudo
     CDS             complement(189..803)
                     /locus_tag="VFC01_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            complement(945..1721)
                     /locus_tag="VFC01_18240"
     CDS             complement(945..1721)
                     /locus_tag="VFC01_18240"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HZN81603.1"
                     /translation="MSAPAVEKDEATTPPVPEGAVMVTLKIARFNPEDPDTAGWQSFR
                     VPCLPTDRLLNLLHYVKWYLDGTLTFRRSCAHGVCGSDAMRINGVNRLACKVLMRDML
                     PKKPDKQLTITIEPIRGLPVEKDLVVNMEPFFDAYRAIKPYLITSGNPPTRERIQSQT
                     DRMRYDDTTKCILCACCTTSCPVYWSEGSYFGPAAIVNAHRFIFDSRDEGAAERLDIL
                     NEVDGVWRCRTTFNCTESCPRGIQVTQAIQEVKRALMFAR"
     gene            complement(1724..>3113)
                     /gene="sdhA"
                     /locus_tag="VFC01_18245"
     CDS             complement(1724..>3113)
                     /gene="sdhA"
                     /locus_tag="VFC01_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HZN81604.1"
                     /translation="DHGKAPVRRACYAADRTGHMILQTLYQNCVKHDVEFFNEFYALD
                     IAMTETPGGPVATGVIAYELASGDIHIFHAKSIVFATGGSGRMYKTTSNAHTLTGDGL
                     GIVFRKGLPLEDMEFHQFHPTGLAGLGILISEAVRGEGGRLLNADGERFMERYAPTIV
                     DLAPRDIVARSMVLEVLEGRGAGPNKDYVYIDVRHLGEDVLEAKLPDITEFARTYLGV
                     DPVKELVPVYPTCHYVMGGIPTTVNGQVLRDNTTIIPGLYAAGECACVSVHGANRLGT
                     NSLLDINVFGRRAGIAASNYALGHDHVDLPADPAGMVVTWVGDILSEHGNERVADIRG
                     ALQQSMDNNAAVFRTEETLKQALTDIHALKERYARISVHDKGKRYNSDLLEAIELGFL
                     LELAEVTVVGALNRKESRGGHAREDYPNRDDTNYMRHTMAYKEGTDLLSDIRLDYKPV
                     VQTRYEPMERKY"
BASE COUNT          491 a         1005 c         1027 g          590 t
ORIGIN      
        1 ggcccggcgg tatgggccag ggtggctgtc gtcgaatcag ctggcgctgg tcaatggtca
       61 gctcggcagc tacaccgtcg aatcgacccc gaccgacggc tatccggcgg tgatgccgcg
      121 cgtcacggcg atggcagtgc gcgacggaaa ggtctgcgcc atatgggata tcgccaaccc
      181 cgacaagttc actggctcgc cgcttgcgac tcctcggtag cccacgggac ccggcaggcg
      241 tcgcccgaat tgaagccctg ctccgtgatg cccagcgccg agtacatgcg cgcccgcatg
      301 ttctccacgc cgatctggta ggtcagctcg atcacgccgt catcgccgaa gcgcttctgg
      361 aggtcggcga cctgctcgtc ggtgatggtg tgcgggtcct tggtcatcgc gtcggcgtag
      421 gcgatcgcgg cccgctcgtc ctcggtgtag tgcggtgacg tcgcatagtc gtcgatgtcc
      481 ttcagccggt ccacgtcgag gccgtcgagg cgctgcagca tcgagccgaa gtcgacgcac
      541 cacgagcatc cgacggtgcg ggcggtccag aacaccgcga gctcgcgcac attcttcggc
      601 agcttcttcg acccggactc cagcagcgtc tcgtgcaccg cgttcgcgat caggagcctc
      661 gggtgatgcg ccgcgacggt gaacggctcg ggcacctcgc caaatcgccg gcgggcgacc
      721 cggtacatga cgcgagtcag caggttggcc ttcttcggcg ggaccggatc gatgcgggtt
      781 ttctgtgtca tacccgttag acgagacagc ctcgcaatct gtgacaacgc tggtaacggc
      841 tcttcatcat ctcgcaggcc tcgaacgtga gcttgtgctc gagattatgg cgatttttcg
      901 caaacaagct cacgttcgac gaagaggcgc ccggaaccga ggcgctagcg cgcgaacatc
      961 agcgcgcgct tgacctcctg gatggcctgc gtcacctgga tgccacgcgg gcaggactcc
     1021 gtgcagttga acgtggtccg gcagcgccat acgccgtcga cctcgttgag gatgtccagt
     1081 cgctccgcgg caccctcgtc gcggctgtcg aagatgaacc tgtgcgcgtt gacgatggcg
     1141 gccgggccga aatagctgcc ctcgctccag tacacggggc agctggtggt gcagcacgcg
     1201 cacaggatgc acttcgtcgt gtcgtcgtag cgcatccggt cggtctggct ctgaatgcgt
     1261 tcgcgcgtgg gcggattgcc gctggtgatc aggtaaggct tgatcgcgcg gtatgcgtcg
     1321 aagaacggct ccatgttgac gacgaggtct ttttccacgg gcagcccgcg gatcggctcg
     1381 atcgtgatgg tcagctgctt gtccggcttc ttcggcagca tgtcgcgcat cagcaccttg
     1441 cacgccagcc ggttcacgcc gttgatccgc atcgcgtccg acccgcacac gccgtgcgcg
     1501 cacgaccgcc ggaacgtcag cgtgccgtcc aggtaccact tcacgtagtg cagcaggttg
     1561 agcagcctgt cggtgggcag gcacgggaca cggaagctct gccagccggc ggtgtcggga
     1621 tcctccgggt tgaaccgggc gatcttcagc gtcaccatca ccgcgccttc gggcacggga
     1681 ggagtggtgg cttcgtcctt ctcgactgcg ggtgcgctca tctctagtac ttccgctcca
     1741 tcggctcata ccgcgtctgc accaccggct tgtagtccag ccggatgtcg gagagcagat
     1801 ccgtgccctc tttgtaggcc atggtgtggc gcatgtagtt ggtgtcgtcg cggttggggt
     1861 agtcctcgcg ggcatggccg ccgcgcgact ccttgcggtt caacgccccg acgaccgtca
     1921 cctcggcaag ctccaacaag aagcccagtt cgatcgcctc gagcagatcg ctgttgtagc
     1981 gtttgccctt gtcgtgcacc gagattcggg cgtaccgctc cttcaacgcg tggatgtccg
     2041 tcaacgcctg cttcaaagtt tcctcggtgc ggaacacggc ggcgttgttg tccatcgact
     2101 gctgcagcgc gccgcgaatg tcggcgacgc gctcgttgcc gtgctcggag aggatgtcgc
     2161 cgacccaggt gaccaccatg cccgccgggt ccgccggtag gtcgacgtgg tcgtggccca
     2221 gtgcgtaatt cgacgcggcg atgccggcgc gacggccgaa cacgttgatg tcgagcagcg
     2281 agttggtgcc cagccggttg gcgccatgca ccgacacgca cgcgcattcc ccagcggcgt
     2341 acaggccggg gatgatcgtc gtattgtcgc gcagcacttg gccgttgacg gtggtcggga
     2401 tgccgcccat cacgtagtgg cacgtcgggt agaccggaac cagctccttg accgggtcga
     2461 cgcccaagta ggtgcgggcg aactcggtga tgtccggcag cttcgcttcg agcacgtctt
     2521 cgccgaggtg gcgcacgtcg atgtagacgt agtccttgtt cgggcctgcg ccgcggcctt
     2581 ccagcacctc gagcaccatc gagcgggcga cgatgtcgcg cggcgccagg tcgacgatcg
     2641 tcggcgcata gcgctccatg aaccgctcac cgtcggcatt caaaagccgg ccgccctcgc
     2701 cgcgcaccgc ctcggagatc aggatgccca gcccagccag gccggtcgga tggaactggt
     2761 gaaactccat gtcctccaac ggaagtccct tgcggaacac gatgcccagg ccatcgccgg
     2821 tgagcgtgtg cgcgttggag gtggtcttgt acatccggcc cgatccgccg gtggcgaaca
     2881 cgatggactt ggcgtggaag atgtggatgt cgccgctggc cagctcgtag gcgatgacac
     2941 cagtggccac cggcccgccc ggcgtctccg tcatcgcgat gtcgagcgcg tagaactcgt
     3001 tgaagaactc gacgtcgtgc ttgacgcagt tttggtacag ggtctgcagg atcatgtgcc
     3061 cggtgcggtc ggccgcgtaa cacgcccggc gcaccggtgc cttgccgtga tca
//
LOCUS       DASRJA010000419        13234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_567522,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000419 DASRJA010000000
VERSION     DASRJA010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13234
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567522"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFC01_18250"
     CDS             <1..367
                     /locus_tag="VFC01_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN81605.1"
                     /translation="DADSSFDLDPGWLSGDPFYIDSLENDPLAFVDADGKPLARELDK
                     AWDRFGSELPKLAVPTLAVHGSNDPIAPVDAVRAYADQLDALQLKEFPGAHHDILNET
                     VHREVAAAIIDFVEKHAGL"
     gene            complement(370..648)
                     /locus_tag="VFC01_18255"
     CDS             complement(370..648)
                     /locus_tag="VFC01_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81606.1"
                     /translation="MGTVMLIIIGSFVIEAIAVAFLVWWRIFRRPRKRQHFGGPWAAG
                     GFVIGGGVDDHGTGSSWGCGGGASHHGGSCGGGGGCGGGGCGGGGGGD"
     gene            718..1191
                     /locus_tag="VFC01_18260"
     CDS             718..1191
                     /locus_tag="VFC01_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81607.1"
                     /translation="MDRMSAAVADLELPPAVFKTCPWQPRELIETGLRQWLRCCAPAL
                     WDNQVIGMPSRAVDEAWHGLILCTARYSAFCTKAYGKFLHHHPEGGAPGGLTVDPIGE
                     QLRRTVVAWTLVATPGERCVLWDLDTRVGVDEPWGLDPARVTAIEEAVAAARGTK"
     gene            1201..1449
                     /locus_tag="VFC01_18265"
     CDS             1201..1449
                     /locus_tag="VFC01_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein"
                     /protein_id="HZN81608.1"
                     /translation="MLKRMVLGAATALAAGTLFAMPANADPGDNPCEFAINYFCKFMP
                     IAPDLEGDIDLTQQQPVDPNAPPPESLPVVDPYAAGCI"
     gene            complement(1446..2012)
                     /locus_tag="VFC01_18270"
     CDS             complement(1446..2012)
                     /locus_tag="VFC01_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZN81609.1"
                     /translation="MTKEPLDPRTFRNVLGQFCTGITVITTMYEGAPVGFACQSFAAL
                     SLEPPLVLFCPTKVSRSWQAIEASGHFCVNVLHEKQKDVSARFGTKEPDKFAGLDWRP
                     SKLGSPIIDGTLAHIDCSVHSVHDGGDHFVVFGAVHSLSDVPHKKPRPLLFYRGEYTG
                     IEPDKNTPAQWRDDLEAFLTTATEDTWL"
     gene            complement(2009..2914)
                     /locus_tag="VFC01_18275"
     CDS             complement(2009..2914)
                     /locus_tag="VFC01_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN81610.1"
                     /translation="MSIRSLGYLRIESTDVAAWREYGLKVLGMVEGKGSTDGALYLRM
                     DEFPARLVIVPGESDRLLESGWECANAEGLQEIRDRLDLEGTPYKEATAAELADRRVD
                     EMIRFQDPSGNCLEIFHGAALEHRRVVSPYGHKFVTEEQGLGHVVLTTRDDAESLHFY
                     RDVLGFRLRDSMRLPPQLVGRPADGPPAWLRFFGVNPRHHSLAFMPGETPSGIVHLMV
                     EVENADDVGLCLDRALRRKVPMSATLGRHVNDKMLSFYMKTPGGFDVEFGCEGLEVED
                     EDWVARESTAVSLWGHDFSVGFKGQ"
     gene            complement(2911..3801)
                     /locus_tag="VFC01_18280"
     CDS             complement(2911..3801)
                     /locus_tag="VFC01_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN81611.1"
                     /translation="MTSFAAETAQQQEITFESTSRYAQVRDDMRLHYHEAGVGNAKTV
                     VLLHGGGPGASSWSNFGRNIAVLAQRFHVLAVDQPGYGHSDKHTEHEQYNRYSATALL
                     NLFDHLDIESADLVGNSLGGGTAVRFALDNGRRAGRLVLMGPGGLSVNLFAPDPTEGV
                     KLLGKFSYEPTRENLEAFLRIMVYDQKLITPELIDERFAIASTEESLAATRAMGKSFA
                     GPDFELGMMWREVYKLRQRVLLIWGREDRVNPLDGALVALKQIPKVQLHVFGQCGHWA
                     QLEKFDEFNKLTIDFLGGGS"
     gene            complement(3801..4985)
                     /locus_tag="VFC01_18285"
     CDS             complement(3801..4985)
                     /locus_tag="VFC01_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent monooxygenase"
                     /protein_id="HZN81612.1"
                     /translation="MTSIEQGDVQSVLAGIDDLLPLIAKRAQATEDLRRLPDETVAEL
                     DEVGFFKLLQPEQWGGLQCDPTLFYEAVRRLATACGSTGWVGSIIGVHNWHLALFDQK
                     AQEEVWGEDPTVRVSSSYAPMGAGTVVDGGYLVNGSWNWSSGCDHATWAFLGGPVIKD
                     GRPVDFGSFLIPRSEYRIDDVWHVVGLRGTGSNTVVVKDVFVPRHRFLSYKSMNDHTA
                     GGLQTNTAPVYKMPWGTVHPTTISAPIVGMAYGAYDAHVEHQGKRVRAAFAGEKAKDD
                     PFAKVRIAEAASDIDAAWRQLIGNVGDEYALLKVDKEIPFELRARARRDQVRATGRAI
                     ASIDRLFEASGATALNNDAPVQRFWRDAHAGRVHAANDPERAYLIFGNNEFGLPPQDT
                     MV"
     gene            5109..6191
                     /locus_tag="VFC01_18290"
     CDS             5109..6191
                     /locus_tag="VFC01_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HZN81613.1"
                     /translation="MAYRKGTAVTDEPLGSHVLELQVADVVEETADARSLVFTVPDGA
                     KIPSEKLRYSPGQFLTLRVPSERTGSVARCYSLCSSPFTGDPMTVTVKRTDNGYASNW
                     LCDNAHAGLKIHVLAPSGTFVPKTLDQDFLLLAAGSGITPMMAICKSALAEGSGKVVL
                     IYANRDENSVIFGGALRELAAKYPDRLTVVHWLETVQGLPSAAALAGLAAPYIGRDAY
                     ICGPGPFMAAAEEALKDSGAPADKIHIEVFKSLESDPFAAVVIAEDDSDEGPATAIVT
                     LDGQRHEVRWPRNAKLLDVLLDKGLDAPFSCREGHCGACAVLKKSGDVEMEVNDVLEQ
                     QDLDEGLILGCQARPRSDSVEVTYDE"
     gene            6280..6777
                     /locus_tag="VFC01_18295"
     CDS             6280..6777
                     /locus_tag="VFC01_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81614.1"
                     /translation="MAVALWMPAVSSAASNNGDSSIPLPDGTTLEMHTTLNCPLPDKQ
                     CYFTTQAQRREGDAVEGFPGDLWARQTTTIRSMNEYNYLETQVVAQNTRVFKAGGTRE
                     ITTIYFGGGPPEKYFISGQIQPTHWQTGQPLLDADVIACAHIQVVYAGVNITSPDACA
                     QTTFS"
     gene            6917..7300
                     /locus_tag="VFC01_18300"
     CDS             6917..7300
                     /locus_tag="VFC01_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN81615.1"
                     /translation="MDDLIQRYTNAWASHDPDAIVALHTEDTRFHAHIGQEPAVGKMA
                     VRQAFAELFAQFPDLAFEQVSLKTTADFWAVEWKMSGTLAGTDHTFEVDLVDLITVED
                     GLVKSKDSYIDAVSMQAQLGLVAVQ"
     gene            7297..7749
                     /locus_tag="VFC01_18305"
     CDS             7297..7749
                     /locus_tag="VFC01_18305"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN81616.1"
                     /translation="MTTSTTNAAEFVEFFEAGWKFGARDAEGFFRHFGPRMHPNTVLI
                     QPIAAPARGPGALRQLFEPLFMAIPDLVGDLKRWGETADGVFIELTLHGHLGGRPVEW
                     TVVDRIILEDGLIRERQSYFDPAPLLKAVALRPRASLPLLLSVFRRKG"
     gene            complement(7746..9857)
                     /locus_tag="VFC01_18310"
     CDS             complement(7746..9857)
                     /locus_tag="VFC01_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN81617.1"
                     /translation="MSATITDEQFAARELVRSWASSSGAIEAVRNVEQGDADAWRAPY
                     RGLAELGIFGVALPEEHGGAGGTADDLCAMVDEAAAAMVPGPVATTALATLVIGEKHA
                     ELLEGLATGERTAGVALSADLRYADGRVSGTADFVLGADAAGVLLLPAGDQWLLVDAA
                     DDGVTVEPLKAADFSRPLARVLLDSAPAEALHVSAQRVEDLAATVLAAEAAGVARWTL
                     QTATEYAKVREQFGKPIGSFQAIKHMCAEMLLRSEQIAVAAADAAAAVANSDERQLSI
                     AAAVAASTAIEAAKANAKDCIQVLGGIGITFEHDAHLYLRRGYGTAQFLGGLPRWLRR
                     VAALTAEGVRRELRIDLDSVADLQPEIASAVAEVAALPAEKQQVALAEAGLLAPHWPR
                     PYGREAGPAEQLLIDQEMAKAGVTRPDLVIGWWAAPTILEHGSPEQIERFVPPTLTGE
                     LFWCQLFSEPGAGSDLASLRMKAVRAEGGWKLTGQKVWTSRADWAQWGVCLARTDADA
                     PKHKGITYFLVDMKSPGIEIRPLREITGDNLFNEVFFDDVFVPDEMVVGQVNDGWRLA
                     RTTLANERVAMAHGTALGNPMEELLRTVSELELDPAVADQLGELIRSAQVGSLLDQRI
                     AQLILGGQDPGPQASARKLIGVRYRQALSEFRMELSEGAGAVENQPVHDFLNTRCLTI
                     AGGTEQILLTLAGERLLGLPR"
     gene            10122..10727
                     /gene="kstR"
                     /locus_tag="VFC01_18315"
     CDS             10122..10727
                     /gene="kstR"
                     /locus_tag="VFC01_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol catabolism transcriptional regulator
                     KstR"
                     /protein_id="HZN81618.1"
                     /translation="MAVLAESELGSEAQRERRKRILDATLAIASKGGYEAVQMRAVAE
                     RADVAVGTLYRYFPSKVHLLVSALGREFERIDAKTDRASLSGGTPYQRLSFMVGKLNR
                     AMQRNPLLTEAMTRAFVFADASAAGEVDHVGKLMDSMFARAMSDGEPTEDQYHIARVI
                     SDVWLSNLLAWLTRRASATDVSKRLDLAVRLLIGDGEHPKI"
     gene            10731..>13234
                     /gene="otsB"
                     /locus_tag="VFC01_18320"
     CDS             10731..>13234
                     /gene="otsB"
                     /locus_tag="VFC01_18320"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HZN81619.1"
                     /translation="MASQLPLELQRALTTVARVPRLLVACDYDGTMAPIVANPDDARP
                     LAESAAALRSLAALPSTTAALISGRALRDLATLSRMPAEVHLVGSHGSEFDTGFVHAI
                     DDAAKALLGKIKDALSAIAAEYAGVAVEIKPASVALHVRNADHADGQEALKKAHTASQ
                     HWDAQVTEGKAVLEFAVIQTDKGQALDILRHQEAASAAVFIGDDVTDEKAFRRLHGPD
                     IGIKVGDGDSLAGYRIESPEDVAAVLECLLEARRTWLLGGHSTPIERLTMLSNARTVA
                     LLTPDADVVWMCHPQADSAAVFSRLLGDATAGHFSIGPQREALPLSQRYITGTMTVET
                     HWASLLVTDYLSHDVGAGRTDLIRVISGQAKAVVEFAPRPEFAQAPVHLRVDEGGLRV
                     FATNEPMVLRAPGVEWEIVPDGIHQTARAVIDPSEGPCVLELRCGTEDLAESPVYQDA
                     RRERAESYWRDWVSTLTLPSLKPELVKRSALTLRGLVHADTGAIMAAATTSLPEEIGG
                     VRNWDYRYCWIRDAAMTASALVSLGSLGEAEGYLNWLHGVIETVHGPERLHPLYALSG
                     AILGPEAVIDSLPGYAGSRPVRVGNAANVQVQLDVFGPVVQLISDLCHRRIADGVTNA
                     LTDPDWNLVSEMVFAVESRWMEPDHGIWEIRDNPRHHVYSKVMCWLTVDRALKLGAEF
                     GREVPAGWAALRDAISEQVRERGWNDEVKSFTAAYDGTDLDAATLYIGLSGLIDPSDE
                     RFAATVVATEAELRSGATVYRYHRDDGLPGTEGGFHLCAAWLLEAYLLSDQRSQAEAL
                     FDRLVAAAGPTGLLSEEYDPVAERALGNHPQAYSHIG"
BASE COUNT         2260 a         4443 c         4305 g         2226 t
ORIGIN      
        1 cgacgctgac agctctttcg atctagatcc cggctggctt tccggtgacc cgttctacat
       61 cgactccctt gagaacgacc cgttggcgtt cgtcgatgcc gatggcaagc cgctggcgag
      121 ggagctcgac aaggcgtggg accggttcgg ctcagagctg cccaagctcg ccgtgccgac
      181 gctggcggtg cacggcagca acgatccgat cgcaccggtc gacgcggtgc gcgcttacgc
      241 ggatcagcta gatgcgttgc agctcaagga atttcccggc gcgcatcacg acattctcaa
      301 tgagaccgtg caccgcgaag tcgcggcggc cattatcgac ttcgtcgaaa agcacgccgg
      361 gctttagagt cagtcgcctc ctccgccgcc accgcaacct ccgccaccac agcctccgcc
      421 gccaccgcaa cttccgccgt ggtgacttgc tccgccgccg cagccccatg aacttccggt
      481 gccgtggtcg tcgacaccgc caccgatgac aaatccgcct gccgcccagg gaccgccgaa
      541 gtgctggcgt ttgcgtggcc gacggaaaat cctccaccac accaggaaag ccacggcgat
      601 cgcctcgatc acaaacgaac cgatgatgat cagcatcacg gtgcccacga gccaaaggct
      661 acgtggctcg gtccctccac atacagtgaa aacgtgaagc tcaagcgcgg cgaccggatg
      721 gatcgcatgt ccgccgcagt ggccgatctc gagctgcccc ctgccgtctt caagacgtgc
      781 ccatggcagc cgcgcgagct gatcgagaca ggtttgcggc agtggctgcg gtgctgtgcg
      841 cccgcgctgt gggacaacca ggtgatcggc atgccgtcgc gcgccgtcga tgaagcatgg
      901 cacggcctga tcctgtgcac cgcgcggtat tcggcgttct gcacgaaggc ctacggtaag
      961 ttccttcacc accatccgga aggcggtgca cccggcggtc tgacggtcga cccgatcgga
     1021 gagcaactgc gcagaaccgt cgtcgcttgg actctggtcg cgacgccagg cgagcggtgt
     1081 gtcctatggg atctggatac gcgagtcggc gtcgatgagc catggggcct tgatccggcc
     1141 cgtgtgacgg cgatcgagga ggccgtcgcc gcggcgcgcg ggaccaagta gggtgacccc
     1201 gtgctgaaga ggatggtgct cggcgcagcg acggcgctgg ccgcggggac tctgttcgcc
     1261 atgccggcga acgccgatcc cggcgacaac ccctgcgaat tcgcgatcaa ctacttctgc
     1321 aagttcatgc ccatcgcgcc ggatctcgaa ggcgacatcg acctcactca gcaacagccg
     1381 gtcgatccca acgcgccacc gccggaatcg cttccggtcg tcgatcccta tgctgcgggc
     1441 tgcatttaga gccaggtgtc ttcggtggcg gtggtgagga acgcctcgag atcgtcgcgc
     1501 cactgcgccg gagtgttctt gtccggctcg attccggtgt actcgccgcg gtagaacagc
     1561 agcggccggg gcttcttgtg cggcacgtct gacagcgagt gcacggcgcc gaacacgacg
     1621 aaatggtcgc cgccgtcgtg cacggagtgc accgagcagt cgatgtgggc cagcgtcccg
     1681 tcgatgattg gcgagccgag tttggaaggg cgccagtcga gtccggcgaa cttatccggc
     1741 tccttggtgc cgaaccgcgc cgagacgtcc ttctgcttct cgtgcagcac gttgacgcag
     1801 aagtgtccgc tggcctcgat ggcctgccat gaccgcgaca ccttggtggg gcagaacagc
     1861 accaacggcg gctccagcga cagcgcagcg aacgactggc acgcaaagcc gactggcgca
     1921 ccctcgtaca tcgtggtgat cacggtgatg cctgtgcaga actgcccgag cacattgcgg
     1981 aatgtgcgcg ggtcgagcgg ttctttggtc actggccctt gaagccgacg ctgaagtcgt
     2041 ggccccacag gctgaccgca gtgctctccc gagccaccca gtcctcgtcc tcgacttcga
     2101 gtccttcgca accgaattcg acgtcgaagc cacccggcgt cttcatgtaa aaggacagca
     2161 tcttgtcgtt gacgtgccga ccaagggtcg ctgacatcgg caccttgcgg cgcagcgcgc
     2221 ggtccaggca cagcccgacg tcgtcggcat tctcgacctc gaccatcaaa tgcacaatgc
     2281 cgcttggtgt ctcacccggc atgaacgcca ggctgtggtg acgcgggttg accccgaaga
     2341 agcgcagcca ggccggtggg ccgtcggcgg gcctgccaac cagttgcggt ggcagccgca
     2401 tcgaatcgcg aagcctgaag cccagcacgt cgcggtagaa gtgcagcgac tccgcatcgt
     2461 cgcgggtggt cagcaccacg tgacccaggc cctgctcctc cgtgacgaac ttatgcccgt
     2521 acggactcac cacgcgtcgg tgctccagcg ccgccccgtg gaagatctcg aggcagttgc
     2581 cggacggatc ctggaaccgg atcatctcgt cgacgcggcg atcggccagc tcggcggcgg
     2641 tggcctcctt gtacggagtg ccttcgaggt ccagccgatc gcgaatctct tgcagccctt
     2701 cggcattggc gcattcccag cccgactcca gcaaccggtc gctctcgccc ggcacgatca
     2761 ccagccgcgc cggaaattcg tccattcgca gatacagcgc cccgtcggtg gaccccttgc
     2821 cctcgaccat gccgagcacc ttcaggccgt actcgcgcca cgccgcgacg tccgtggatt
     2881 cgatccgcag atagcccagt gaccggatgc tcatgacccg cctcccagga agtcaatggt
     2941 cagcttgttg aactcgtcga acttctccag ctgtgcccag tgcccgcact gcccgaagac
     3001 gtgcagctgg accttcggga tctgtttgag ggccaccaac gcaccgtcga gcggattgac
     3061 gcggtcttcc cggccccaaa tgagaagtac ccgctggcgc agcttgtaca cctcgcgcca
     3121 catcatgccg agctcgaagt cggggcccgc gaacgacttt cccatcgccc gggtggcggc
     3181 cagcgattct tcagtgctgg cgatcgcgaa gcgttcgtcg atcagctcgg gtgtgatcag
     3241 cttctggtcg tacaccatga tccgcaggaa cgcctcgagg ttctcacggg tcggctcgta
     3301 cgagaacttg ccgagcagct tgacgccctc ggtgggatcc ggcgcgaaca ggttcaccga
     3361 caggccgccg ggccccatca gtacgagccg gccggcgcgc ctcccgttgt ccaacgcgaa
     3421 ccgcaccgca gtgccgccgc cgagcgaatt tccaaccagg tcagcgcttt cgatgtcgag
     3481 gtggtcgaac aggttcagca gcgcggtggc actgtagcgg ttgtactgct cgtgttcggt
     3541 gtgcttgtca gagtgtccgt agccgggctg atcgacggcc agcacatgaa agcgctgcgc
     3601 cagcacggcg atattgcggc cgaagttgga ccagctcgac gcgccaggcc caccgccgtg
     3661 cagcagcacg accgtcttcg cgttcccaac tccggcctcg tggtagtgca gccgcatgtc
     3721 gtcgcgtacc tgcgcgtagc gggaggtcga ctcgaatgtg atctcttgct gttgtgcggt
     3781 ttcggcggcg aacgacgtca tcagaccatc gtgtcctgcg gcggcaggcc gaactcgttg
     3841 ttgccgaaga tcaggtaggc gcgttcggga tcgttggccg cgtgcacccg gccggcgtgc
     3901 gcgtcgcgcc agaaccgttg caccggagcg tcgttgttca gtgcggtagc gccagacgcc
     3961 tcgaagagcc ggtcgatcga cgcgatggcg cgcccggtgg cgcggacctg gtcgcgacga
     4021 gcgcgggcac gcagctcgaa cggaatctcc ttgtccacct tcaacagtgc gtactcgtcg
     4081 ccgacgttgc cgatcaactg ccgccatgcc gcatcgatgt cgctagcagc ctcggcgatg
     4141 cggaccttcg cgaacggatc gtccttggcc ttctcccccg cgaacgccgc gcgcacgcgc
     4201 ttgccctggt gttcgacgtg ggcgtcgtag gcgccgtacg ccatccccac gatcggtgcc
     4261 gagatagtcg tgggatgcac agtgccccaa ggcatcttgt agacaggcgc ggtgttggtc
     4321 tgcagaccgc ccgcggtgtg gtcgttcatc gacttatacg acaggaaacg gtgccgcggc
     4381 acgaacacgt ctttcaccac aacggtgtta ctgccggtgc cgcgcaggcc gacgacatgc
     4441 cagacgtcgt cgatccggta ctcgctgcgc ggaatcagga agctgccgaa gtcgaccggc
     4501 ctgccgtcct tgatgaccgg cccaccgagg aacgcccacg tggcgtggtc gcagcccgac
     4561 gaccagttcc aggaaccgtt gacgaggtag ccgccgtcga cgacggtgcc tgcgcccatc
     4621 ggggcgtacg acgacgagac gcgaaccgtc ggatcctcgc cccacacctc ttcttgcgcc
     4681 ttctggtcga acaacgcgag atgccagttg tgcacgccga tgatcgagcc gacccagccg
     4741 gtggacccgc aggcggtggc gagacgccgg accgcttcgt agaacagtgt cgggtcgcat
     4801 tgcaggccgc cccactgctc gggctgcaga agcttgaaga aaccgacctc gtcgagctcg
     4861 gccacggtct catcgggcag ccgccgcagg tcttccgtcg cctgggcgcg cttggcgatc
     4921 agcggtaaca ggtcgtcgat gccggctagg accgactgca cgtcaccctg ttcaatggac
     4981 gtcactggat ttgcctcccg ggaatacgga cctagaggaa gattagaaca cgttacgatt
     5041 tgtgtcgagc agcgcgttcc tgcggcggct ggacctgcgc acatgtattt ctgtaacctg
     5101 ttctagttat cgcctacagg aagggcacgg ctgtgacgga cgagccgctc ggtagccacg
     5161 tgctcgagct gcaggtggcc gacgtcgtcg aggagacggc cgacgcgcgg tcgctggtgt
     5221 tcaccgtgcc cgacggcgca aagatcccgt ccgaaaaact ccgctattca cccgggcagt
     5281 tcctaacgct gcgggtgccc agcgagcgca ccggctcggt ggcccgctgc tactcgctgt
     5341 gcagctcgcc gttcaccggt gacccgatga ccgtcaccgt caagcgcacc gacaacgggt
     5401 acgcgtcgaa ctggctttgc gacaacgcac atgccgggct gaagatccac gtgctcgccc
     5461 cgtcgggcac cttcgtgccc aagacgctgg accaagactt cttgctgctg gccgctggca
     5521 gcggcatcac cccaatgatg gcgatctgca agtccgcact tgccgagggc agcggcaagg
     5581 tcgtgctgat ctacgccaac cgcgatgaga actcggtgat cttcggcggc gcgctgcgcg
     5641 agcttgcggc caaatatccc gaccggctca ccgtcgtgca ctggctcgaa acggtgcagg
     5701 gtctgccgag tgcggcggcg ctggccggcc tggccgcgcc gtacatcggg cgcgacgcat
     5761 acatctgtgg gccggggccg ttcatggccg ccgccgagga agcgctgaaa gactctggcg
     5821 cacctgcgga caagatccac atcgaggtgt tcaagtcgct ggagtccgac ccgttcgctg
     5881 cagtcgtcat cgcggaggac gacagcgacg aaggacccgc caccgcgatc gtcacgctcg
     5941 acggccagcg ccatgaggtg cgctggccgc gcaacgccaa attgctcgac gtgctgctcg
     6001 acaagggcct cgacgcgccg ttctcctgcc gcgagggcca ttgcggcgca tgcgcggtgc
     6061 tgaagaagag cggcgacgtc gagatggagg tcaacgacgt gctcgaacag caggacctcg
     6121 acgaggggtt gatcctgggg tgccaggccc gaccgcgatc ggattcagtc gaagtcacct
     6181 acgacgaata acccgcgggc taccatttcg ccgcaccgga gggagcaaac cgtgaagcgg
     6241 ttgagcagtc ttgtcgggtc gctgtgcgcg ggtgggacga tggccgtcgc gctgtggatg
     6301 ccggcggtct catcggccgc atccaacaac ggcgactcgt cgatcccgtt gcccgacggc
     6361 accactctgg agatgcacac cacgctcaac tgtccgctac ccgataagca gtgctacttc
     6421 acgacgcaag cccaacgacg cgagggcgac gccgtcgagg gcttccctgg ggacttatgg
     6481 gcacggcaga cgaccacgat ccgctcgatg aacgagtaca actatctgga gacacaggtc
     6541 gtcgcccaga acacccgggt cttcaaggcc ggcggcacac gcgaaatcac tacgatctac
     6601 ttcggcggcg gcccgccgga gaagtacttc atcagcggcc agatccagcc cacacactgg
     6661 cagaccggcc agccgctgct ggacgccgac gtgatcgcct gcgcccatat tcaggtcgtc
     6721 tatgccggcg tgaacatcac ttcgccggac gcctgcgcgc agaccacatt cagctagcgc
     6781 cgcgctctcg aaactgcggg gagatcgcag aaacgcgcca gaggacgatc tggccgcagt
     6841 ttcggcgtac ccattgacat acagactgcc ggtatgtaac ttccatactg cctgtacgca
     6901 actgagagga gcgcttgtgg acgatctgat ccagcgctac accaacgctt gggcgagcca
     6961 cgatcccgac gcgatcgtcg ccctgcacac ggaagacacc cgcttccatg cgcatatcgg
     7021 ccaagagcca gccgtcggca aaatggctgt gcgacaagca tttgccgaat tgttcgcgca
     7081 attccccgac ctcgctttcg agcaggtctc gctgaagacc acagccgact tctgggcggt
     7141 cgagtggaag atgagcggaa ctcttgctgg taccgatcac acgtttgagg tcgacctggt
     7201 cgacctgatc accgtcgagg acggcttggt caagagcaag gacagctaca tcgacgccgt
     7261 gtcgatgcag gcacagctcg ggctggtcgc cgtgcaatga ccacatccac aacaaacgcc
     7321 gctgagttcg tcgagttctt cgaggcgggc tggaagttcg gcgcacgcga tgcggagggc
     7381 ttcttccggc acttcgggcc gcgcatgcac ccgaacaccg tgctgattca gccgatcgcg
     7441 gcgcccgccc gcggccccgg tgcgttgcgc cagcttttcg agccgctctt catggccatc
     7501 cccgacctcg tcggcgacct gaagcgctgg ggcgagacgg ccgacggggt gttcatcgag
     7561 ctgaccctgc acggtcacct cggcggcaga ccggtcgagt ggaccgtcgt cgaccgaatc
     7621 atcttggagg acggcctgat tcgcgaacgg cagtcctact tcgacccggc gccgctgctc
     7681 aaggcggtgg ccctgcggcc gcgcgcctcg ctgccgctgc tactcagcgt cttccggagg
     7741 aagggctagc gcggcagccc gagcagccgc tcgccggcga gcgtgagcag gatctgctcg
     7801 gtaccgcccg cgatggtcag gcagcgggtg ttgaggaagt cgtggacggg ctggttctcg
     7861 acggctccgg caccctccga gagctccatc cggaactccg agagcgcctg ccggtaccgc
     7921 acgccaatca gcttgcgcgc gctggcttgt gggccggggt cctgaccgcc gaggatcaac
     7981 tgcgcgatcc gctgatccag tagcgagccc acctgtgcag accggatcag ctcccccagc
     8041 tggtctgcga cggccgggtc gagctcgagt tcggacaccg tccggagcag ctcttccatc
     8101 gggttgccca gcgcggtgcc gtgggccatc gcaacacgtt cgttggcaag cgtggtgcgg
     8161 gccagccgcc aaccgtcgtt gacctgcccg accaccatct cgtcgggcac aaagacgtcg
     8221 tcgaagaaca cctcgttgaa aaggttgtcg ccggtgattt cgcgcagcgg ccggatctcg
     8281 atgcccggcg atttcatgtc gacgaggaag tacgtgatgc ccttgtgctt cggcgcgtcg
     8341 gcgtcggtgc gcgccaggca gacgccccat tgcgcccagt ccgcccgtga cgtccacacc
     8401 ttctgccccg tgagcttcca accgccctcg gcgcgaacag ctttcatccg cagtgaggcg
     8461 aggtcggagc ctgcgcccgg ctcgctgaac aactggcacc agaacagctc accggtcagc
     8521 gtgggaggca cgaaccgctc gatctgctcg gggctgccgt gctcgaggat cgtcggcgcg
     8581 gcccaccagc cgatcaccag gtcggggcgc gtcacgccgg ccttcgccat ttcctgatcg
     8641 atcagcagct gctcagctgg cccggcttcg cggccgtacg gtcgcggcca gtgcggtgcc
     8701 aacagcccgg cctcggcaag ggccacttgc tgtttctcgg ccggcaacgc ggcgacttcg
     8761 gccaccgcag aagcgatttc gggctgcaga tcggcaaccg agtccagatc gatgcgcagt
     8821 tcgcggcgca cgccctcagc cgtcaacgcc gcaacacggc gcagccacct gggcaggccg
     8881 ccgaggaact gcgcagtccc atacccacgg cgcagataca ggtgggcgtc gtgttcaaac
     8941 gtgatcccga tgccgccgag cacctgaatg cagtccttcg cattggcctt cgccgcttcg
     9001 atcgcggtcg acgccgcaac cgccgcggcg atggacagtt ggcgttcatc ggaattcgcc
     9061 acagcggcgg cggcatccgc ggccgccacc gcaatctgtt cggagcgcaa cagcatctcg
     9121 gcgcacatgt gcttgatggc ctggaagctg ccgatcggct tgccgaactg ttcgcgcacc
     9181 tttgcgtact cggtggcggt ctgcagcgtc caccgggcga cccccgcggc ctcggcggcc
     9241 agcaccgtcg cggcgaggtc ctcgacccgc tgtgccgaca cgtgcagtgc ctctgccgga
     9301 gccgagtcca gcagcacccg cgccagcggg cgggaaaagt ccgcggcctt cagcggttcg
     9361 acggtcacgc cgtcgtccgc cgcatcgacg agcaaccact ggtcgcctgc gggcagcaac
     9421 agcacccccg ccgcgtcggc gccgagcacg aaatcggcgg tacccgacac ccggccgtcg
     9481 gcgtagcgca ggtcggcgga cagcgcgacg cccgcggtgc gttcccccgt cgccaaccct
     9541 tcgagcagct cggcgtgttt ctcaccaatg accagcgtgg ccagcgcagt ggtggcaact
     9601 gggccgggca ccatcgctgc ggcggcctcg tcgaccatcg cgcacaggtc atcggcggtg
     9661 ccgcccgcgc cgccatgctc ctcgggaagc gcgacgccga agatccccag ctcagccagg
     9721 cctcggtagg gagctcgcca cgcgtcggca tcgccttgct ccacattgcg cacagcctcg
     9781 atcgcaccgg aactggacgc ccagctacgt accagctcgc gtgcggcaaa ctgctcatca
     9841 gtgatggtgg ccgacaccgt gactctccta ccgtttgggc gagaagagca tgcttctcac
     9901 tagaacgtgt tctaatagtg ccagcgttcg accgtcaagt cgaccgccat tagagcagga
     9961 cacgtcggta ccgcagcggc gccgaggtga gaaaatggag ccgcgcatgc gtatcgtttt
    10021 caccgagtac gcacgatgaa ggagcagccg accgcatgtc gtcaccagca cagcccaaca
    10081 cgggttcggg gtcggagtcg cggccgcgcc aggtgttgaa cgtggccgta ctggcggagt
    10141 ccgagctcgg gtccgaagcc cagcgtgagc gccgcaagcg catcctcgac gccaccctgg
    10201 ccatcgcctc aaagggcggc tacgaggcgg ttcagatgcg cgcggtcgcc gaacgcgccg
    10261 atgttgcggt cggcacgctg tatcgctact tcccgtcgaa ggtgcacctg ctggtgtccg
    10321 cactcggccg ggagttcgag cgcatcgacg ccaagaccga ccgggcgtcg ctgtcgggcg
    10381 gcacgccgta tcagcggctg agcttcatgg tcggaaagct gaaccgtgcg atgcagcgca
    10441 accccctgct gaccgaggcc atgacccgtg cgttcgtgtt cgccgacgcg tcggcggccg
    10501 gtgaggttga tcacgtcggc aagctgatgg actcgatgtt cgcccgcgcc atgagcgacg
    10561 gcgaaccgac cgaggaccag taccacatcg cgcgcgtcat ctcggatgtc tggctgtcca
    10621 acctgctggc ctggttaacg cggcgggcgt cggcgaccga cgtgagcaag cggctggacc
    10681 tggctgtgcg tctgctcatc ggagacggcg agcaccctaa gatttagcgg gtggcttccc
    10741 agctacccct tgagctccag cgcgcgctga ccaccgtcgc acgggtaccg cggctgctgg
    10801 tggcgtgcga ctacgacggc accatggcac cgatcgtcgc gaaccccgac gatgcgcgcc
    10861 cgctcgccga atcggcggca gccctccgct cgctggccgc cctgccgtcg acgaccgccg
    10921 cgctgatctc cggccgcgcc ctgcgcgacc tggcgacgct gtcgcggatg cccgccgagg
    10981 tccatctggt gggcagtcac gggtcggagt tcgacaccgg tttcgtacac gcgatcgacg
    11041 atgcggccaa ggccctgctg ggcaagatca aagacgccct gagcgcgatc gccgccgagt
    11101 acgccggcgt ggccgtcgag atcaagccgg ccagcgtggc cttgcatgta cgcaacgcgg
    11161 atcacgccga cggccaggag gcgctgaaga aggcgcacac cgcctcgcaa cattgggacg
    11221 cgcaggtcac cgagggcaag gccgtcctgg agttcgcggt catccagacc gacaaggggc
    11281 aggcactcga cattctgcgg caccaggagg ccgcctccgc ggcggtattc atcggcgatg
    11341 acgtcaccga cgagaaggcg tttcgccgac tgcacgggcc cgacatcggc atcaaggtcg
    11401 gcgatggcga ctccctggcc ggttatcgca tcgagtcgcc cgaagacgtc gccgccgttt
    11461 tggaatgcct actcgaggcg cgccgcacct ggctcttggg cggacacagc acgccgatcg
    11521 agcggctcac gatgctgtcc aacgcccgca cggtggcact gctgacaccc gatgcggacg
    11581 tggtctggat gtgccatccg caggccgact cggctgccgt gttctcccgg ctgctcggcg
    11641 acgcgaccgc aggacacttc tcgatcggtc cgcagcgcga ggccctaccg ctgtcccagc
    11701 gctacatcac cggcacgatg accgtcgaaa ctcattgggc cagtttgctt gtcaccgact
    11761 acctgtcgca tgacgtcggc gccggccgta ccgacctgat tcgggtcatc agcgggcagg
    11821 ctaaggcggt ggtcgagttc gcgccgcggc ccgagttcgc gcaggctccc gtccatctgc
    11881 gggtcgacga gggcgggctg cgggtgttcg cgaccaacga gccgatggtg ttgcgcgcgc
    11941 ccggcgtgga gtgggaaatt gtgcccgacg gtatccacca gaccgcacgc gctgtcatcg
    12001 atccgtccga gggaccgtgt gtgctggaat tacggtgtgg caccgaggat ttggcggaga
    12061 gcccggtata tcaagatgcg cggcgcgaac gtgcggagag ctactggcgc gactgggtgt
    12121 ccacgctgac tctgccgtcg ctgaaacctg agctggtgaa gcggtcggca ctgacgctgc
    12181 gcgggttggt gcacgccgac accggcgcga tcatggcggc ggccaccacg tcgctacccg
    12241 aggagatcgg cggcgtccgc aactgggact accgttactg ctggatccgc gacgccgcga
    12301 tgaccgcgtc ggccctggtg tcgttgggtt cgctcggcga ggccgaggga tacctgaact
    12361 ggctgcacgg tgtgatcgag acggtgcacg ggcccgagcg gctgcatccg ctgtacgcgc
    12421 tgtccggggc gatcctcggc ccggaggcgg tgatcgactc gctgcccggg tatgcgggtt
    12481 cgcgccccgt gcgagtcggc aacgccgcga atgtccaagt gcaactggat gttttcggtc
    12541 ctgtcgtgca gctgatatcg gacttgtgtc atcggcggat agccgacggt gtgacgaacg
    12601 ccttgacgga tccggactgg aatctggtca gcgagatggt gttcgccgta gaaagtcgtt
    12661 ggatggaacc ggatcacggc atctgggaga ttcgcgacaa tccgcggcac cacgtgtact
    12721 cgaaggtgat gtgttggctg actgtggacc gcgcgctgaa gctgggtgct gaattcggcc
    12781 gcgaggtgcc agccggttgg gccgcgctgc gcgacgcgat ctcggagcag gtgcgcgagc
    12841 gcggctggaa cgacgaggtg aagtccttca ctgccgcgta cgacggcacc gacctcgacg
    12901 cggccacgct gtatatcggg ctgtccggcc tgatcgaccc gtccgatgag cggttcgcgg
    12961 ccacggtagt cgcgacggag gctgaactgc gcagcggtgc aacggtttac cgctatcacc
    13021 gcgacgacgg cctgccgggc accgagggcg gcttccatct ctgcgcggcg tggctgctcg
    13081 aggcgtactt gctatccgat cagcggtcgc aggccgaggc gctgttcgat cggctggtcg
    13141 ccgcagcggg gccgacgggg ctgttgtccg aggagtacga cccggtggcc gagcgggcgt
    13201 tgggcaacca tccgcaggcg tacagccaca tcgg
//
LOCUS       DASRJA010000420         7111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1970544,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000420 DASRJA010000000
VERSION     DASRJA010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970544"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..943)
                     /gene="urtB"
                     /locus_tag="VFC01_18325"
     CDS             complement(59..943)
                     /gene="urtB"
                     /locus_tag="VFC01_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HZN81620.1"
                     /translation="MDVLIGQLATGLSLGSILLLAALGLSLTFGQMGVINMAHGEFIM
                     AGCYTAYVVQQLVSSAGVSLLISLLVGFIVGGAMGALLEVTLIRRMYNRPLDTLLVTF
                     GVGLILQQVARDIFGAPAVNVIAPSWLSGGVEILGAVVPKTRIFILVLAVVCVAVLAI
                     VLKVSPMGRRIRAVVQNRDLAETSGISSRKTDITTFFIGSGLAAVAGVALTLIGSTSP
                     TIGQSYLIDAFLVVVVGGLGQIKGTVIAAFALGFLNSFIEYSTTASLAKVIVFVIIVI
                     FLQVRPQGLFTVRTRSLA"
     gene            complement(945..2210)
                     /gene="urtA"
                     /locus_tag="VFC01_18330"
     CDS             complement(945..2210)
                     /gene="urtA"
                     /locus_tag="VFC01_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HZN81621.1"
                     /translation="MRLPGRSSLKRSALAAGSVVAATSLLLSGCGSKATESDSANAAS
                     CVDTSAPTIKVGALNSLSGTMAISEVTVRHAIDLAVEQINAAGGVLGKQIQLVGEDGA
                     SEPTVFAEKAQKLISSDCVAVVFGGWTSSSRKAMLPVFESANSLLYYPVQYEGLESSK
                     NIFYTGATTNQQIVPALDYLKEKGIKSLYLVGSDYVFPQTANRIIKAYAGANGIEIKG
                     EDYTPLGSTDFSTIVNKVRGSNAGAVFNTLNGDSNVAFFKEYTNAGLTPQKMPVVSVS
                     IAEEEVTGIGAKNIQGQLTAWNYYQTVDNPVNKAFVKAYKDKYGADKPTSDPMEAAYV
                     SVYLWKNTVEKAKSFDVKAIQDNCGGVTFDAPEGLVTIDGENHHITKTARIGEIHPDG
                     LIYTIWQSQGPIKPDPYLKTYPWAAGLSS"
     gene            2456..2875
                     /locus_tag="VFC01_18335"
     CDS             2456..2875
                     /locus_tag="VFC01_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81622.1"
                     /translation="MRTTTARVGAALAVAAAAIAVSHGTASAEPAGHLVRYTLTTGGP
                     ADFDLFYLVNQPPNKQAYNADAYSFVKKETVNVGPGAPWVFETTLADPQWAIFTVSST
                     THGGQAAPNPHCEIAVDGQVVVQQDAPYNLQCQGSQW"
     gene            complement(2880..2990)
                     /locus_tag="VFC01_18340"
                     /pseudo
     CDS             complement(2880..2990)
                     /locus_tag="VFC01_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016894700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
     gene            complement(3021..3908)
                     /locus_tag="VFC01_18345"
     CDS             complement(3021..3908)
                     /locus_tag="VFC01_18345"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HZN81623.1"
                     /translation="MAGARPYGQLQYKQINGKRMAYIDEGQGDAIVFQHGNPTSSYLW
                     RNVMPHLEGLGRLVACDLIGMGGSDKLDDSGPDRYYYAEHRDFLFTLWDELDLGDRVI
                     LVLHDWGSALGFDWANRHRDRVAGIAYMEAVAMPIEWSDFPGPMRSVFQGFRSPDGES
                     MVLEQNMFVEAVLPASVKRQLTDQEMDHYREPFRNPGEDRRPTLSWPRNIPIEGEPAD
                     VVATVEEYGTWLSQSDVPKLFINGEPGALIRGRARDFVRTWPNQTEVSVAGVHFLQED
                     SPDEIGAAVASFVRAVRAA"
     gene            complement(3936..5180)
                     /locus_tag="VFC01_18350"
     CDS             complement(3936..5180)
                     /locus_tag="VFC01_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZN81624.1"
                     /translation="MPAAQTALVIGGGIAGPVAATALTMAGIDATVYEARVSDPSSAN
                     GIGGSLALEPNGLAALRIIGADDAVRAAAIPITRSVMSIASKPGREMPTPQGLGPRQL
                     IDRGALHRILRDRAEQAGVRIQDAKKLIRVDELPDCVTAHFADGSTASADMVIGADGV
                     HSKVRKLIDPNAPGARYLNLLGFEGIAEDAPDTSALEPGAMTFAFGRRGYYLYWKRPD
                     GRIGWGANLPSPQYLSLKEARAVPAEDWLHTLRTAYANDVPGARLASETNSETLQVVG
                     ALHIMPPVPHWHRGRMVLVGDAVHAPSNSTGQGASLAMESAIELARCLRDMTDVPSAF
                     SVYERLRRPRVEKIAARGARISHAKAPGPIGQRMMRLLLPVIVKTMNIEKTMAAEQRY
                     TIDWNSHVERELIAHFSRETVS"
     gene            complement(5190..5816)
                     /locus_tag="VFC01_18355"
     CDS             complement(5190..5816)
                     /locus_tag="VFC01_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZN81625.1"
                     /translation="MAEKRKVANLMGLAVLATVVQRPMHRYEMASVMRARGKDRDMDI
                     KWGSLYTVVQNLERNGYLETIGVTRQGARPERTVYQITDAGRDELIAWTRELIAEPEA
                     EQTRFVAGLSVLAVLNPQDVVELLRRRLERLTQSVDSLTALLETASDVPRLFLIEDEY
                     RIAMTRAEADWVRSLLDELTAGTFPHLDAWRSFHETGQMPPEFADLEK"
     gene            5920..6939
                     /locus_tag="VFC01_18360"
     CDS             5920..6939
                     /locus_tag="VFC01_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2578c family radical SAM protein"
                     /protein_id="HZN81626.1"
                     /translation="MVRWDRQGVSVDDGALPGLQRIGFVRSVRTPQFEGITFHEVLCK
                     SALNKVPDAAMLPFRYTVNGYRGCSHACRYCFARPTHEYLDLDCGTDFDTQVVVKTNV
                     ADVLRRELRRPSWQRETVALGTNTDPYQRAEGRYALMPGIIGALADSGTPFSILTKGT
                     LLRRDLPLITAAAKTVDVSVAVSLAVGDPALHKDVEPGTPSPQARLGLIAAIREAGLG
                     CHVMVAPVLPRLTDSVEHLDALLAQIAAAGATGVTVFGLHLRGSTRGWFMAWLAKSHP
                     ELVGEYRELYRRGAYLPSDYKKMLHDRATPLVAKYGLAPDRRSFRTTEAPAVPPAEVL
                     QPTLF"
BASE COUNT         1234 a         2300 c         2284 g         1293 t
ORIGIN      
        1 agcagcactg ccgccacgcc gaatcccgcc caggtgtgcc agcggcccaa aagggttctc
       61 acgcgagact cctcgtccga acggtgaaca gaccctgcgg gcggacctgc aggaagatca
      121 cgatgatcac gaacacgatc accttggcca gcgacgccgt ggtgctgtac tcgatgaacg
      181 agttcagaaa acccaacgcg aacgccgcga tcacggtgcc cttgatctgc cccagtccgc
      241 cgaccaccac gacaaggaac gcgtcaatca gataactctg gccgatggtc gggctcgtcg
      301 aaccgatcag cgtcagcgcc acacccgcaa ccgcggccag ccccgacccg atgaaaaacg
      361 ttgtgatgtc tgtctttcgc gacgagatgc cgctggtctc ggcgaggtcg cggttttgta
      421 ccacggcccg gatccgccgc cccatcgggc tgaccttcaa gacgatggcg agcacggcca
      481 cgcacaccac cgccagcacc aggatgaaga tgcgggtctt tggcaccacc gcgcccagga
      541 tctccacacc gcccgataac cagctcggcg caatgacgtt gacggccggg gcgccgaaga
      601 tatcgcgggc cacctgctgc aggatcagcc cgacgccaaa cgtgaccagc agggtgtcca
      661 gcggccggtt atacatccgc cggatcagcg tcacctccag cagcgcgccc atggcgccgc
      721 ctacgatgaa gccgacgagc aacgaaatca acagggacac accggcgctc gagacgagct
      781 gctgtaccac gtaagccgtg tagcagcccg ccatgatgaa ctcgccatgc gccatgttga
      841 tgacgcccat ctgcccgaac gtcagtgaca accccagagc ggccaacaac aggatcgagc
      901 cgaggctcaa tcccgttgcc agctgtccaa tcaggacatc catctcagct agaaagaccc
      961 gcggcccacg ggtacgtctt caggtatggg tcgggcttga tcggtccctg cgactgccag
     1021 atcgtgtaaa tcagcccgtc gggatggatc tcgccgatac gcgcggtctt ggtgatgtgg
     1081 tggttctcgc catcgatggt gaccaggccc tcgggtgcgt caaaggtgac cccgccgcaa
     1141 ttgtcctgaa tcgccttgac gtcgaacgac tttgccttct cgacggtgtt cttccacagg
     1201 tacaccgaga cgtaggcggc ctccatcgga tccgacgtcg gcttatcggc gccgtacttg
     1261 tccttgtagg ccttgacgaa cgccttgttc accgggttgt cgaccgtctg gtagtagttc
     1321 cacgcggtca gctggccctg gatgttcttg gcgccgatgc ccgtgacctc ttcttcggca
     1381 atcgacacgg acaccaccgg catcttctgc ggcgtcagac cggcgttggt gtactccttg
     1441 aagaacgcga cgttcgaatc gccattgagg gtgttgaaga ccgcgccggc gttggagcca
     1501 cggaccttgt tgacgatggt ggagaagtcc gtcgagccca gaggcgtgta gtcctcaccc
     1561 ttgatctcga tgccattggc cccggcgtac gccttgatga ttcggttggc ggtctgcggg
     1621 aagacgtagt cactgccgac cagatacagc gacttgatgc ccttttcctt gaggtaatcg
     1681 agcgcgggca cgatctgctg gttggtcgtg gcgccggtat agaagatgtt cttcgacgac
     1741 tcgaggccct cgtactgcac ggggtagtac agcagcgagt tggcgctttc gaagaccggc
     1801 agcatcgcct tgcggctcga tgaggtccag ccgccgaaaa ccaccgccac acagtcgctg
     1861 ctgatcagtt tctgggcctt ttcggcgaac acggtcggct cggacgcacc gtcctcgccg
     1921 acgagctgaa tctgcttgcc caacacgccg cccgcggcgt tgatctgctc gacagccaga
     1981 tcgatggcat ggcgcacggt cacttcggag atcgccatgg tgcccgagag cgagttcagc
     2041 gcacccactt tgatggtcgg tgccgaggtg tccacgcacg acgcggcgtt tgccgagtcc
     2101 gattcggtcg ccttgcttcc gcagccggac aacaacaggc tggtcgcggc gacgacgctt
     2161 cccgcagcga gagccgaccg tttcaacgag gagcgtcctg gtagtcgcat ggacagcctt
     2221 tcttgtcgtc accagcgcac atccgggtgg tgagcgttgg gttggagcgc agaagtaaga
     2281 caccgacgag gcgtcgaatc gggacgttag atcgcctgtg tttctacgaa atgtctgggt
     2341 gtgacgaaca aattaacgtg tcacccggcg ggagttggtt tgccgttcgc ccggtgcggc
     2401 atagctgagg tgtcctcttc gtaacgatcg ggtacggtgc ggcgatgtga cggacatgcg
     2461 aacaacgaca gccagggtcg gggcggcatt ggcggtggcg gccgccgcaa ttgcggtaag
     2521 tcatggcacg gcgagcgctg agccggccgg tcacctagtg cgctacacgc tcacgacagg
     2581 tggccccgcg gatttcgacc tcttctatct cgtcaatcag ccgccgaaca agcaggcata
     2641 taacgcagac gcgtactcct tcgtgaagaa ggagaccgta aacgtggggc ctggtgcgcc
     2701 atgggtgttc gagaccacgc ttgcggaccc gcagtgggcg atcttcaccg tgagcagcac
     2761 cacgcacggc gggcaggccg cgccgaatcc tcactgcgag atcgccgtcg acggccaagt
     2821 ggtcgtgcag caggatgctc cttacaacct gcagtgccag ggttcgcagt ggtgagcctc
     2881 ccggcgaatt cgtataccca cgcttgatcg cctgagcgca gtggcaccgc gatgcggcgg
     2941 tagtcgtcgg cctcgtactc gtcggcggcg gccaaatcgg cggcgctgat gtagtccagg
     3001 ttccgtcagc ctgccgaaga ttacgcggcg cgcaccgcgc ggacgaacga cgcgacggcc
     3061 gcgccgattt cgtcggggct gtcctcctga aggaaatgca caccggccac cgagacttcg
     3121 gtctgattag gccaggtccg gacgaagtcg cgggcgcgcc cgcggatcag tgcgccgggt
     3181 tcgccgttga tgaacagctt gggcacatcg ctttgtgaca gccacgtgcc gtactcctcg
     3241 acggtggcga ctacgtcggc gggttcaccc tcgatcggga tgttgcgcgg ccacgacaag
     3301 gtcggcctgc ggtcctcgcc gggatttcgg aacggctcgc ggtagtgatc catctcctga
     3361 tcggtgagtt gacgcttgac ggacgcgggc agcaccgcct ccacgaacat gttctgctcc
     3421 aacaccattg actcgccgtc gggggagcgg aagccctgga acacgctgcg catcgggcca
     3481 ggaaaatcgg accactcgat cggcattgcg accgcctcca tgtaggcgat cccggccacc
     3541 cggtcgcggt gccggttcgc ccagtcgaag cccagcgccg aaccccagtc gtgcagcacc
     3601 agaatcaccc gatcgccgag gtcgagctcg tcccacaacg tgaagagaaa gtcgcggtgt
     3661 tcggcgtagt agtagcgatc cggtcctgag tcgtcgagct tgtccgagcc gcccatgccg
     3721 atcaggtcgc acgcgacaag gcggcccaga ccctcgagat gcggcatgac gttgcgccac
     3781 agatacgacg acgtcggatt gccgtgctgg aagacgatcg cgtcgccttg gccctcatcg
     3841 atgtaggcca tccgcttgcc gttgatctgc ttgtactgca gctgcccgta cggcctggca
     3901 ccggccattg cccctcctag tccgcgaatg tgaacttatg acacagtttc gcgcgaaaag
     3961 tgtgcaataa gttcacgttc gacatgacta ttccagtcga tggtgtagcg ctgctcggcg
     4021 gccatcgtct tctcgatgtt catcgtcttg actatgaccg gcagcagcag ccgcatcatc
     4081 cgctgaccga tcgggccagg ggccttggcg tggctgatcc gggcgccgcg cgcggcgatc
     4141 ttctcgaccc tcggtcggcg cagtcgctcg taaacggaga acgccgacgg aacatccgtc
     4201 atgtcgcgta gacaccgggc cagctcgatc gcactctcca tcgccagcga ggcgccctgc
     4261 ccggtgctgt ttgatggtgc gtgcacggcg tcgccgacga gcaccatccg gccccggtgc
     4321 cagtggggga ccggcggcat gatgtgcagc gcgccgacga cttgcagcgt ctcgctgttc
     4381 gtctcggagg ccagccgagc cccgggaacg tcgttggcgt aggcggtgcg cagcgtgtgc
     4441 agccagtcct ctgctgggac ggctcgcgcc tccttcaggc tcagatattg gggcgacggc
     4501 agattcgcac cccagccgat gcggccgtcg ggccgcttcc aatacaggta gtacccccgc
     4561 cgcccgaacg cgaacgtcat ggcgcctggt tcgagtgcag aggtgtcggg ggcgtcctca
     4621 gcgatgccct cgaatccaag caggttgaga tagcgggcgc ctggcgcgtt cgggtcgatg
     4681 agcttgcgca ccttcgaatg cacgccgtcg gcgccgataa ccatgtcggc gctggcggtg
     4741 ctgccgtcgg cgaagtgcgc ggtgacacag tcgggcagtt cgtcgacgcg aatcagcttc
     4801 ttggcgtctt gaattcgcac accggcctgc tcggcgcgat ccctgaggat gcgatgcagc
     4861 gcgccgcggt cgatcagctg acgggggcca aggccctggg gcgtcggcat ctcgcgcccg
     4921 ggcttgcttg cgatcgacat gaccgagcgg gtgattggga tcgcggcggc gcggacggcg
     4981 tcgtcggcgc ctatgatccg cagggcagcc aggccattgg gctcgagtgc cagcgagccg
     5041 ccgatcccgt tggccgagga cggatctgac acgcgcgcct cgtagacggt ggcgtcgatg
     5101 ccggccatcg tcagcgcggt ggccgcgacc ggtccggcga ttccgccacc gatcacgagt
     5161 gcggtttgtg ctgcgggcat gaggtctcct tacttctcca ggtcggcgaa ttcgggtggc
     5221 atttggccgg tttcatggaa gctgcgccat gcgtcgagat gcgggaaggt gccggccgtc
     5281 agttcgtcga gcagcgaccg cacccagtcg gcctcggcgc gcgtcatcgc gatccggtat
     5341 tcgtcctcga tcaggaacaa ccgtggtacg tccgacgccg tctcgagcag cgcggtcaac
     5401 gagtcgaccg attgcgtcag ccgctccaag cgccggcgca gtagctccac aacgtcctgc
     5461 gggttgagca ccgccagcac ggacaggccc gcgacgaagc gggtctgctc ggcctccggt
     5521 tcggcgatca gctcgcgggt ccacgcgatg agttcgtcgc gcccggcgtc ggtgatctga
     5581 tacaccgtgc gctcgggccg agccccttgg cgggtaacac cgatcgtctc gaggtagccg
     5641 ttgcgctcca ggttctgcac gacggtgtag agcgaccccc acttgatgtc catatcgcgg
     5701 tccttgccgc gggcccgcat caccgatgcc atctcgtagc ggtgcatcgg tcgctgtacg
     5761 accgtggcca gcacagcgag ccccatcagg ttcgcgacct tgcgtttctc ggccatcaca
     5821 cctcctggca attactcgca cacgaatata cgcatacgag tatcgccgac acaacggggt
     5881 aattaccttg acatcacatc gaacaagagt tcgaatggaa tggtgcgatg ggatcggcag
     5941 ggcgtcagcg tcgatgacgg cgccctgcct ggcctgcagc gcatcggctt cgtccggtcg
     6001 gttcgcacgc cgcagttcga gggcatcacg ttccacgagg tgctctgcaa gagcgcgttg
     6061 aacaaggtgc ccgacgcggc gatgctgccg tttcgctaca ccgtcaacgg ctaccgcggc
     6121 tgttcgcatg cctgtcgcta ctgcttcgct cggcccacac acgagtacct cgatctggac
     6181 tgcggcaccg atttcgacac ccaggtggtg gtgaagacca acgtcgccga tgtgttgcgt
     6241 cgcgaactac gccggccgtc gtggcaacgc gagaccgtcg cacttggcac caacaccgat
     6301 ccctatcagc gggccgaggg gcgctacgcg ctgatgcccg gcatcatcgg cgcgttggcc
     6361 gattccggca cgccgttttc catcctcacc aagggcaccc tgctgcgccg cgatctgccg
     6421 ctgatcaccg cggcggccaa aaccgtcgac gtgagcgtcg cggtgtcgct cgcggtcggc
     6481 gatcccgcgc tgcacaagga cgtcgagccg ggcacgccct cgcctcaggc caggctcggg
     6541 ttgatcgccg ccattcgtga ggccggcctt gggtgtcacg tgatggtcgc gccggtgctg
     6601 ccgcgattga ccgactccgt cgagcacctc gatgcgctgc tggcccagat cgccgcggcg
     6661 ggcgcgacgg gtgtgacggt gttcggcctg catctgcgtg gctcgacgcg aggctggttc
     6721 atggcctggt tggcgaaatc gcacccggag ttagtggggg agtaccgcga gttgtacagg
     6781 cgcggagcgt atctaccctc ggactacaag aagatgctgc acgaccgcgc gacgccgttg
     6841 gtcgccaagt acggtctggc acccgaccgc cggtcgttca gaacgactga ggccccggcg
     6901 gttccgcccg ccgaggtcct gcagccgaca ctgttctagc gctcgttgaa gccgagatcg
     6961 gcgaaacgcg gcaccggttg gccgcgccgc gccgcctccg cgtccagtcg cttgttcaac
     7021 cggcggccgg ccgagagcat gtagatggtc cggcggatgg agttcttcgg cacgatgaag
     7081 tcgttgaggg ttcgctctgc gtggaaatcg g
//
LOCUS       DASRJA010000421         4508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_285976,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000421 DASRJA010000000
VERSION     DASRJA010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285976"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..2167
                     /locus_tag="VFC01_18365"
     CDS             20..2167
                     /locus_tag="VFC01_18365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZN81627.1"
                     /translation="MAENTIQWDKDADGIVTLTLDDPTGSANVMNEHYAESMHNAVER
                     LVAEKDSITGVVITSAKKTFFAGGDLKGMINLGPENAGEAFDTVEAVKRDLRALETLG
                     KPVVAAINGAALGGGLEITLACHHRIAADVKGLVVGFPEVTLGLLPGGGGVTRSVRMF
                     GIQNAFMNVLSQGTRFKPDKAKEAGLVDELVGSVEELVPAAKAWIKANPDSHTQPWDQ
                     KGYKMPGGTPSSPGLAGILPSFPALLRKQLKGAPMPAPRAILDAAVEGAQVDFDTASR
                     IESRYFTQLVTGQVSKNMIQAFFFDLQHINGGGSRPEGIGKTPINKIGVLGAGMMGAG
                     IAYVSAKAGFDVVLKDVSIEAAQKGKGYAEKLEAKALQRGKTTEEKSKALLDRITPTA
                     DPADFKGVDFVIEAVFENQELKHKVFQEIEDVVEPNALLGSNTSTLPITGLATGVKRQ
                     EDFIGIHFFSPVDKMPLVEIIKGEKTSDEALARVFDYTLAIGKTPIVVNDSRGFFTSR
                     VIGTFVNEALAMLGEGVEPASIEHAGSQAGYPAPPLQLSDELNLELMHKIATATRKGV
                     EDAGGKYEPHPAEAVVEKMIEIGRPSRLKGAGFYEYVDGKRTGLWPGLRETFQSGSNQ
                     QPLQDMIDRMLFAEALETQKCLDEGVLTSTADANIGSIMGIGFPPYTGGSAQFIVGYQ
                     GPAGTGKDAFVARAKELAAKYGDRFTPPDSLTS"
     gene            complement(2216..3886)
                     /locus_tag="VFC01_18370"
     CDS             complement(2216..3886)
                     /locus_tag="VFC01_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN81628.1"
                     /translation="MRRWLLNTGGSTFHWWLRLTVAAFLLATWVAVGLGSVIAHADDP
                     SPSSADSSSESSAGAPKAAKADPPKRTAQVGAQDAGTDRKPDLRTPESSREDTATAEK
                     TASDSDAPDVDVQPSLSASVADPDPDDSPQPRVRHSPRRPDVPIASAKPAKAVADEPQ
                     PSAIDVTIDRAAVAPTPPTPDPQPVAAKIASTAPTVSSTQSGGTTIRAPQPREVVGLI
                     SDVGVVAASAVYTVADTIAKAFGPHSFLGVPYALATAVANSAAAVSRTLIGAPLNSAG
                     TGPFPVTYGIINGLTFLNPQQPPPGANDELISVTAEHPLPIILINGTIATQGENWGVG
                     APVLANAGYKVYTFNYGNITQNPNYPIQATDDIRQSAQQLAAEVDKVLKETGAPKVIL
                     IGHSQGGGILPVYYINNLGGADKVSQLIGIAPSNHGTDVSGLIGLQDLPILGPLLIAF
                     ANSIGPALEQQALGNEFQQEVYGNGDTRPGVLYTTIASKTDWVVTPYTQQALDGPNVT
                     NIVLQDLYPGLPASHLGVVLSPQVWSVVLDALASNPAANPVHSEELVA"
     gene            4112..4402
                     /locus_tag="VFC01_18375"
     CDS             4112..4402
                     /locus_tag="VFC01_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81629.1"
                     /translation="MKRCIVGGLTALLMAAELIASAPPATAGCQYGGPVLSKCDGPVQ
                     PDGTWQRCVAVPQLIPRGASSYLVPVRHCELMGPDQHPWDLAFADPPTHLDD"
BASE COUNT          797 a         1427 c         1496 g          788 t
ORIGIN      
        1 atcgagaggg tttaagacga tggcagagaa caccattcag tgggataagg atgccgacgg
       61 catcgtcacc ctgacgctcg acgatccgac cgggtcggcg aacgtgatga acgagcacta
      121 cgccgagtcc atgcacaatg ctgtcgaacg ccttgtcgcg gaaaaggatt cgatcaccgg
      181 cgtcgtcatc accagcgcga agaagacctt cttcgccggt ggcgacctca agggcatgat
      241 caacctcggc ccggaaaacg ccggtgaggc gttcgacacc gtcgaggccg tcaagcgtga
      301 cctgcgcgcc ctcgaaacgc tcggcaagcc ggtggtggcc gccatcaacg gcgccgcgct
      361 cggtggtggg ctggagatca cgctcgcgtg tcaccaccgc atcgcggccg acgtcaaggg
      421 ccttgtcgtg ggcttccccg aggtgaccct tggcctgctg cccggcggtg gcggcgtcac
      481 ccgcagcgtg cggatgttcg gcatccagaa cgccttcatg aacgtcctgt cgcagggcac
      541 ccgcttcaag cccgacaagg ccaaggaggc cggcctggtc gacgaactgg tcggctccgt
      601 cgaggagttg gtgcccgccg ccaaggcgtg gatcaaggcg aaccccgaca gccacaccca
      661 gccgtgggac cagaagggct acaagatgcc cggcggcacc ccgagttcgc cgggtctggc
      721 gggcatcctg ccgtcgttcc cggcgctgct gcgcaagcag ctcaagggtg cgccgatgcc
      781 cgcgccacgc gccatcctgg acgcggccgt cgagggtgcg caggtggact tcgacaccgc
      841 gtcgcgcatc gagagccgct acttcacgca actggtcacc ggccaggtgt cgaagaacat
      901 gatccaggcg ttcttcttcg acctgcagca catcaacggc ggtggctcgc ggcccgaggg
      961 catcggcaag accccgatca acaagatcgg cgtgctgggc gcgggcatga tgggcgccgg
     1021 catcgcctac gtgtcggcca aggccggctt cgacgtcgtg ctcaaggacg tcagcatcga
     1081 ggccgcgcag aagggcaagg gctacgcgga gaagctcgag gccaaggcgc tgcagcgcgg
     1141 caagaccacc gaggagaagt ccaaggcgct gcttgatcgc atcacgccga cggccgaccc
     1201 cgcggacttc aagggcgtcg acttcgtgat cgaggcggtg ttcgagaacc aggaactcaa
     1261 gcacaaggtg ttccaggaga tcgaggacgt cgtcgagccc aacgcgctgc tcgggtcgaa
     1321 cacctcgacg ctgccgatca ccggtctggc gaccggcgtg aagcggcagg aggacttcat
     1381 cgggatccac ttcttctcgc cggtcgacaa gatgccgctg gtggaaatca tcaagggcga
     1441 gaagacatct gacgaggcgc tggcccgggt gttcgactac acgctggcca tcggcaagac
     1501 cccgatcgtc gtcaacgaca gccgcggctt cttcaccagc cgcgtcatcg gcacgttcgt
     1561 caacgaggcg ctggcgatgc tcggcgaagg cgtggagccg gccagcatcg aacacgccgg
     1621 ttcgcaggcc ggttatcccg cgccgccgct gcagctgtcg gacgagctga acctcgagct
     1681 gatgcacaag atcgcgaccg ccacccgcaa gggcgtcgag gacgcgggcg gcaagtacga
     1741 gccgcatccg gccgaggcgg tcgtcgagaa gatgatcgag atcggtcggc cgtcacggtt
     1801 gaagggcgcc ggcttctacg agtacgtcga cggcaagcgc accggtctgt ggccgggctt
     1861 gcgtgagacg ttccagtccg gcagcaacca gcagccgctg caggacatga tcgaccggat
     1921 gttgttcgcc gaggcgctgg agacgcagaa gtgcctcgac gagggcgtgc tcacgtcgac
     1981 cgccgacgcg aacatcggct cgatcatggg catcggtttc ccgccgtaca ccggtgggtc
     2041 ggcgcagttc atcgtcggct atcaaggccc ggcagggacc ggtaaggacg ccttcgtggc
     2101 gcgggccaag gagctggccg ccaagtacgg cgaccgcttc accccgccgg actcactgac
     2161 cagctagcag tagtacgcga aagccctcgg aacaacggtg tttcgggggc ttttgctatg
     2221 cgaccagttc ctcgctatgc accggattag ccgctgggtt gctcgctaat gcgtccagaa
     2281 ccacggacca cacctgtggg ctcaacacca cgccaaggtg gctggccggg agtcccggat
     2341 acagatcctg aaggacgata ttggtcacgt tcgggccgtc gagcgcctgc tgggtgtacg
     2401 gggtgactac ccaatcagtc ttcgaggcga tggtggtgta cagaacaccg gggcgggtgt
     2461 cgccgtttcc gtacacctct tgctgaaatt cattgcctaa tgcctgctgt tccaacgccg
     2521 gcccaatact gttcgcgaac gcgatgagca gcggccccag aatcggcaag tcctgcaagc
     2581 cgatcaggcc ggagacatcg gtgccgtggt tactcggcgc gatgccgatg agctgtgaca
     2641 ccttgtcggc gccgcccaga ttgttgatgt agtacacggg caggattccg ccgccctgcg
     2701 aatggccgat gagtatcacc ttgggcgccc cggtttcctt gagcaccttg tccacttctg
     2761 cggcaagctg ctgcgcggac tgcctgatgt catccgtggc ctggatcggg tagttgggat
     2821 tctgcgtgat gttgccgtag ttgaacgtgt agaccttgta gccggcgttg gcgaggacgg
     2881 gtgctccgac gccccagttc tctccctggg tggcaatcgt tccgttgatg agaatgatcg
     2941 gcaggggatg ctcggcggtg accgagatca gctcgtcgtt cgcgccgggc ggcggctgtt
     3001 gtgggttgag gaacgtcagc ccgttgatga tcccgtaggt gacggggaac ggtcccgtgc
     3061 cggcggaatt gagcggcgca ccgatcagtg tcctgcttac ggccgcggcc gaattcgcca
     3121 ccgcggtggc caacgcatag ggcacaccga ggaagctgtg cggtccgaat gccttggcga
     3181 ttgtgtcggc cacggtgtac accgccgacg ccgctacaac gcccacgtct gatatcagcc
     3241 caacgacttc ccgaggctgc ggggctctga tcgtggtccc gccggattgc gtgcttgaca
     3301 ccgtgggggc ggtggaagcg atcttcgccg ccacgggctg tgggtcggga gtcggtggtg
     3361 tgggcgcgac cgcggcgcga tcaatcgtga catcgatcgc ggacggctgc ggctcgtcgg
     3421 cgactgcctt cgccggctta gccgatgcga tgggcacgtc gggtcggcgc gggctgtgcc
     3481 gcacgcgcgg ctgcggcgag tcgtctgggt ccggatccgc taccgatgct gacaatgacg
     3541 gctgaacgtc gacgtcgggc gcgtcgctat cgctcgccgt cttctcagcg gttgcggtgt
     3601 cctcgcgcga cgattcgggg gttcggaggt cgggtttgcg gtcggtaccg gcatcctgcg
     3661 cgcccacctg cgcggtccgt ttcggcgggt cggccttcgc agctttcggc gcgcctgccg
     3721 agctctccga actcgaatca gcactcgaag gtgacgggtc atcggcatgg gcgataacgc
     3781 tgcctaaccc gaccgccacc caggttgcca gcaagaacgc ggcaaccgtc aaccgcaacc
     3841 accagtgaaa ggtcgacccg ccggtattca gtagccacct gcgcatcgtc gacattgcac
     3901 gcctttcgcc cggttggcat gcaatgtatc cagttaacgg ccgggttggt tctgattggc
     3961 aatgctcgtg ttgcttcgga tcaccacctc aaaggcggca aaaaccagtt acataagcgg
     4021 tagcgtcatt ggttgctggc tgggctgtcg acggtatttg ccaccccagg cgcccgacaa
     4081 ctccccagcg gcgatgtgac gggagacaac catgaagcgc tgcatcgtcg gaggattgac
     4141 cgccctgctg atggccgcag agctgatcgc ctcagcgccg ccggccaccg ccggctgcca
     4201 gtacggagga cccgttctca gcaagtgcga tggacccgtc cagcctgacg gcacctggca
     4261 acgttgcgtg gcagttcccc aattgattcc tcgcggcgcc agctcctacc tagtgcccgt
     4321 caggcactgc gaattgatgg gtcctgacca gcacccctgg gatctcgcct tcgccgaccc
     4381 accgacgcac ctcgacgact gagtcgcaag gggagctgaa gattgcagtc agggctgtgg
     4441 attgcggcgc gccacgaccc tctgtgcaat cttcgtgatg cggccacttt ggctatcgtc
     4501 tagtcgtg
//
LOCUS       DASRJA010000422         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3057651,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000422 DASRJA010000000
VERSION     DASRJA010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3057651"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1303)
                     /gene="arcA"
                     /locus_tag="VFC01_18380"
     CDS             complement(92..1303)
                     /gene="arcA"
                     /locus_tag="VFC01_18380"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HZN81630.1"
                     /translation="MLDVTLGCDSEVGRLRAVILHRPGAELQRLTPRNNDALLFDGLP
                     WVSKAQEEHDAFTAVLESRAVEVLLLADLLTEALSHSGAARMHGISAAVDSRRLGLPL
                     AQELSAYLRTLEAGELAHVLMAGMTFNELPLSGGELSLVRRMHHGGDFVIEPLPNLLF
                     TRDSSFWIGPRVAITSLALPARVRETSLTDLIYAHHPRFLGVRRAYESRSAPVEGGDV
                     LLLSPGVVAVGVGERTTPAGAEALARSLFDDDLAHTVLAVPIAQERAQMHLDTVCTMV
                     DVDAAVMYPNIVDSLSAFTIHRDGSGGVRIDDAAPFVEAAAAAMQIEKLRVIATGLDP
                     VTAEREQWDDGNNTLALAPGVVVAYERNTETNERLADAGIEVLPIAASELGTGRGGPR
                     CMSCPAARDPL"
     gene            1341..1775
                     /gene="soxR"
                     /locus_tag="VFC01_18385"
     CDS             1341..1775
                     /gene="soxR"
                     /locus_tag="VFC01_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HZN81631.1"
                     /translation="MELIHELTPGEMSHRSGVAVSALHFYEREGLIASQRTAGNQRRY
                     ARETLRRVAFIRMSQRLGIPLARIRDALKSLPTDRVPTSKDWAKLSAGWRQDLDDRIL
                     HLERLRDNLAGCIGCGCLSLKTCALSNPGDVLADQGPGAVRL"
     gene            1809..>2299
                     /locus_tag="VFC01_18390"
     CDS             1809..>2299
                     /locus_tag="VFC01_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HZN81632.1"
                     /translation="MELALQSALFDPAERRDLGNGAWVEVRSGWLTDADGLFAELHDG
                     IPWRAERRHMYERVLDVPRLVSFHHLVDEPAPHPRLKQMRRRLNDTYGGELGEPFTTA
                     GLCLYRDGNDSVAWHGDKIGRSNTEDTMIAIVSLGATRVFALRPKGGGKSLRIAHHHG
                     DLLV"
BASE COUNT          421 a          857 c          683 g          338 t
ORIGIN      
        1 ccgccgcggg tggaagccac caaaattgca ctgagggctg tgaattcggc gcaatcacag
       61 ccctgggtgc aaaattgagc ggcacaatct gctagagcgg gtcgcgggcc gccgggcatg
      121 acatgcagcg cgggccgccg cggccggtgc ccagctcgga cgccgcgatc ggcagcacct
      181 cgatgcccgc atcggcaagc cgctcattgg tttccgtgtt gcgctcgtat gcgaccacca
      241 cacccggcgc cagcgcgagc gtgttgttgc cgtcgtccca ctgctcacgc tcggccgtca
      301 ccgggtccag ccccgtcgcg atgacccgca gcttctcgat ttgcatcgcc gcggcggccg
      361 cctccacaaa cggtgccgca tcgtcgatcc tcacaccccc ggaaccgtcg cggtgaatcg
      421 tgaacgccga caacgagtcc acgatgttcg ggtacatcac cgccgcatcg acgtcgacca
      481 tcgtgcacac cgtgtccaga tgcatctgtg cgcgctcctg cgcgatcggc accgccagaa
      541 ccgtatgcgc caaatcatcg tcgaacaggc tgcgcgccaa cgcttccgcg cccgcgggcg
      601 tggtccgctc ccccacgccg accgcaacca cacccggcga aagcagcagc acgtcgccgc
      661 cctcgacggg tgccgaccgc gactcgtatg cccgccgcac gcccaggaac cgcgggtgat
      721 gcgcgtagat caggtcggtc agcgacgtct cgcgcacccg cgcaggcagt gccagcgatg
      781 tgatcgccac ccgcggcccg atccagaacg acgagtcccg cgtgaacaac aggttcggca
      841 gcggctcgat cacaaaatcc ccgccgtggt gcatccgccg caccagcgac agctcaccac
      901 cggacaatgg caactcgttg aacgtcatcc ccgccatcag gacgtgcgcc agctcgcccg
      961 cttccagtgt ccgcaagtac gccgaaagct cttgcgccag cggcagtccc aaccgccgag
     1021 aatcgacagc ggccgaaatt ccgtgcatcc gcgccgcgcc actgtgcgac agcgcctcgg
     1081 tcaacagatc cgcgagcaac agcacctcga cggcgcgcga ttccaacacc gccgtgaacg
     1141 cgtcgtgctc ctcctgcgcc ttggacaccc acggcagccc gtcgaacagc agcgcgtcgt
     1201 tgtttcgtgg tgtcagccgc tgcaattcgg ccccgggccg gtgcaggatg accgcccgca
     1261 gcctgccgac ctccgaatcg caacccaacg tcacatctag caccgtagcg ccaccgttca
     1321 cctcaactat ggtttaggtc atggagctga tccacgaact cactccgggc gagatgtcgc
     1381 atcgcagcgg cgtggccgtc tctgcgctgc atttctacga acgcgaaggc ctgatcgcca
     1441 gccagcgcac tgccggcaac caacgccggt acgcgcggga aacactgcgg cgcgtcgcgt
     1501 tcatccggat gtcgcagcgc ctcggcatcc cgctcgcccg catccgcgac gcactcaaga
     1561 gcctgcccac cgaccgcgtt ccgaccagca aggactgggc caaactgtcg gcgggctggc
     1621 gccaggatct cgacgaccgc atcctgcacc tggagcggct gcgcgacaac ctcgccggct
     1681 gcatcggctg cggctgcctg agcctgaaga cctgcgcgct gtccaacccc ggcgacgtac
     1741 tcgccgacca gggacccggc gccgtgcggc tgtaagaaat cgaacgcctg tgcgatagac
     1801 tcgcgatcat ggagctggcc ctccaaagcg cactgttcga ccccgccgaa cgccgcgatc
     1861 tcggcaacgg cgcgtgggtc gaggtgcgct ccggttggct caccgacgcc gacgggctgt
     1921 tcgcagaact ccacgacggc atcccgtggc gcgccgaacg caggcacatg tacgagcgcg
     1981 tgctcgacgt gccacgtctg gtcagcttcc accacctcgt cgacgagccg gccccgcacc
     2041 cacggctcaa gcagatgcgc cgacggctca acgacaccta cggaggcgag cttggcgagc
     2101 cgttcaccac cgcaggtcta tgcctttacc gcgacggcaa cgacagcgtc gcatggcacg
     2161 gcgacaagat cggccgcagc aataccgaag acaccatgat cgctatcgtg agcctcggtg
     2221 ccacaagggt tttcgcgtta cgcccgaaag gcggtggcaa gtcgctgcgg atcgcacacc
     2281 atcacggcga cctgttggt
//
LOCUS       DASRJA010000423        22919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2339069,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000423 DASRJA010000000
VERSION     DASRJA010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22919
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339069"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="VFC01_18395"
     CDS             complement(<1..1413)
                     /locus_tag="VFC01_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HZN81633.1"
                     /translation="MSGSPLTAAALLAAAVATVAIGAYGVRLSRNTSDFLVASRSVGP
                     QWNAAAISGEYLSAASFLGVAGLIAKYGADALWYPVGFTAGYLGLLLFVAAPLRRSGA
                     YTVPDFAEFRLGSARLRKVAMLVVVVICVFYLVPQYQGAGLALKTLLGVPVWIGPLVV
                     GGIVIANVVAGGMRSITFVQAFQYWLKLTAIAIPALALLGLFFTERGELGGPMPPTVQ
                     QHTTVAIETDVVVQVAAPAGITVTGMLDGNHVDAQPIPSAGEHTLGAGTTLTLTAGAA
                     TPVVAGTPSTGKAWIASGGGLGGVHPLYQVVSIIVATFLGTMGLPHVLVRFYTNPDGR
                     AARRTALAVIALLSLFYLFPTLLGAFARLSVPQLLITGTADAAVLLAPGAAIGGPAGQ
                     LLAALVAAGAIAAFLATSSGLLVSIAGALSTDVLRGRVRDFRVAAVVGGLIPIPLSLA
                     TSGLELSRSVGLAFAVAASTL"
     gene            complement(1410..1784)
                     /locus_tag="VFC01_18400"
     CDS             complement(1410..1784)
                     /locus_tag="VFC01_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81634.1"
                     /translation="MTSGERPQRERVVLAHRRGARMVRTRVEVQEQTLVGDALVRGLV
                     RAQLGLALRLAAVVVCTVGAIPLLNAVFPDLGAVTVLGMRLNWLVLAVLVYPLLYGIG
                     RVYVRLAEQAERDFVGVVDSEP"
     gene            complement(1771..2538)
                     /locus_tag="VFC01_18405"
     CDS             complement(1771..2538)
                     /locus_tag="VFC01_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZN81635.1"
                     /translation="MNRALTVLAVDDEAPALDELAYLLGRHPEIGQVYRAGDATSALR
                     ELNSRTIDAIFLDINMPGLSGIELAGVLANFSHRPLVVFVTAHDDKAIAAFDVGAIDY
                     LLKPIREDRLDEAVRRVVEARPDPSEDGHDDVIPAELGGVTHLVPRDTIGWVEAEGDY
                     ARLHSASGSHLVRIPLTTLESRWRDRGFQRVHRSYLVALRLVTGLRTSDGAVLVRLRA
                     NGASPAVELPVSRRQARELRDRLVRDPMRNLRPDDQR"
     gene            complement(2535..3719)
                     /locus_tag="VFC01_18410"
     CDS             complement(2535..3719)
                     /locus_tag="VFC01_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZN81636.1"
                     /translation="MSGELAIALAAALILAAVAAAVIAVRTRRVVATPTERAVHAALN
                     IASLAARALRRGLDTESAQTAAPFLRGLTGTDGVALFDGEGRLLAQDPPDESNRDACQ
                     RAALESISGQRRVLTHGQASQVIAQPLLEEGGELLGVLVVVTTRSPGPGMLGAVGEVA
                     RYAASQIELAQLDASRARLDRAEVLALRAQISPHFIYNALNTIASFVRTDPDRARELI
                     LEFADFTRYSFRAAGQYTTLAEELRNIDRYLNLERARFGTALKVRLQVAPEVLNVVVP
                     FLALQPLVENAVRHGFAGQSKGSVEIVARDEGADCVITVEDDGVGMDPEILRTGPGDA
                     LADGSGAAAHVGLTNVDHRLRAAFGNDYGLVVETAVGAGTKVVMRVPKFRSGVRASGG
                     VE"
     gene            3751..4329
                     /locus_tag="VFC01_18415"
     CDS             3751..4329
                     /locus_tag="VFC01_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HZN81637.1"
                     /translation="MAKLQLVQDPAADALLESNPFALLVGMLLDQQYPMEAAFAGPKK
                     IADRIGGVDPREIADYDPEEFAALCSKTPAIHRFPGSMAKRIQTLAQIVVDQYDGEAA
                     ALWTAGAPDGAEVLRRLKSLPGFGEQKAKIFLALLGKQYGVTPKGWREAAGNYGTAGS
                     YLSVADVTDAGSLEKVRSYKKQAKAAAKAAKG"
     gene            complement(4326..4712)
                     /locus_tag="VFC01_18420"
     CDS             complement(4326..4712)
                     /locus_tag="VFC01_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZN81638.1"
                     /translation="MTCTLGYATASTAGQNLEGQLAALNAAGVDPRRVFTDKLSDSAE
                     RVRAGLHAMLSYARSGDTVIVVAIDRLGRSAAEVARTIAELTDRGIRLQALKEGLDTG
                     TSTGRVVASVITSLAKLDLEHERQTS"
     gene            complement(4760..5443)
                     /locus_tag="VFC01_18425"
     CDS             complement(4760..5443)
                     /locus_tag="VFC01_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZN81639.1"
                     /translation="MELSEVMRTTGAVREYTDDPLPDEVLERILDNARFAPSGGNRQG
                     AHVVVVRDQQTRDRLADLAIPGARRYIAQLRNGEGPWNPLQPCGVDAETIAAVEVPPG
                     SPLRDAPVVLVVCVNLAVVAAFDQDLDRIGVIPGASVYPFVWNILLAARNEGYGGVLT
                     TAAVAEEPRLKELLGIPEDFAVAALLPLGKPVRQLRKLTRKPVAELATRERFDGEPFD
                     NSSAVSQTR"
     gene            5479..5625
                     /locus_tag="VFC01_18430"
     CDS             5479..5625
                     /locus_tag="VFC01_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HZN81640.1"
                     /translation="MKTHLNCPCGEAITGKDEDDLVEKAQAHLSEAHPGREYDRDAIL
                     FMAY"
     gene            complement(5705..6622)
                     /locus_tag="VFC01_18435"
     CDS             complement(5705..6622)
                     /locus_tag="VFC01_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81641.1"
                     /translation="MRSVRTTLAGAVVASTALLLGGMTPVANAEPAPPLPIDALQAPG
                     LTAVQSIGPAIQQAAADPTNAASTLMAAAAVFAGNSAMPADSQNVATAVNQFVAHVPA
                     PGAVPGEEAHLPAGVNPAHAVGPVPEVAPVVAPAPEAAPAPAPAPAPAPASAPDPAAA
                     PAAAPAFGPDAPPTQDFLYPSISNGCLADGGNVLATAISVAGPARIPAPGPGPGQTAY
                     VFTAVGTPAPAAEQKLPLNVTWVNLTTGKSGSAALKPRPDINPNGPTTLTAIVDTGSG
                     SIMSTIFGQVTTTEKQCQFMPTIGSTVVP"
     gene            complement(6667..7218)
                     /locus_tag="VFC01_18440"
     CDS             complement(6667..7218)
                     /locus_tag="VFC01_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81642.1"
                     /translation="MKFTRKMLATSMIAAAGAIPIALGLSATAAAEPAAPAPTPGVPG
                     LPFLQQLATNPAAATTLMQGLTSVLSGVQSAAPAAAPAAATAPAPAATASVSLPQPPA
                     PAAVPAAAAAAPATAPQNLLTQGLSVPSALASLMPAGTPLAGLLPAAATAPAAAAAPA
                     AAAPAAASTAPLAPMMMPLSALP"
     gene            7396..8589
                     /locus_tag="VFC01_18445"
     CDS             7396..8589
                     /locus_tag="VFC01_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase"
                     /protein_id="HZN81643.1"
                     /translation="MPARLAAAAPVLLAISIAARLAMTYLVPHGANFVDLHVYVGGAE
                     MLEQHGKLYDYVYADQTPDFPLPFTYPPFAAVVFYPLHFIPFGILTFAWQVAIIASLY
                     GVVRISQRLLSGEGDRRIAMLWTALGIWLEPMRSNFDYGQINVMLVLAVLYAVYCTRW
                     WLSGLLVGLAAGVKLTPAVSGLYFVGARRWATAVFSAVAFFGTIAVSALVVGDQTRYY
                     FTELLGDARRVGPIGTSFNQSWRGGISRILGYDAGYGPLVVAAIVVTALLAILAWRAI
                     GGADDRLGAIVVVQLFGLLSSPISWTHHWVWLIPLMIWLLHGPLRERLGARVLGWGWL
                     ALTIVGVPWLLSFAQPTIWVIPRPWYLAWAGLIYIVATLATLAWIAASRRSGLSRAPC
                     PDRSG"
     gene            complement(8537..8818)
                     /locus_tag="VFC01_18450"
     CDS             complement(8537..8818)
                     /locus_tag="VFC01_18450"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZN81644.1"
                     /translation="MAVLTDDQVDAALPDLDGWQRSQGAIRRSIKFPSFMDGIEAVRR
                     VAARAEEKDHHPDIDIRWRTVTFALVTHAAGGITQNDLDMAHEINLIVG"
     gene            9239..10348
                     /locus_tag="VFC01_18455"
     CDS             9239..10348
                     /locus_tag="VFC01_18455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003917712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN81645.1"
                     /translation="MITRGWPTAAVRSGYLRAALAGVDTYWMPDHLNSLVPRSIAVPK
                     YVGLARVVPDVDAVCEPWTLLGQVAGWNRIGRLRLGVAVTDGGRRNPAVTAQAAATLH
                     LMSRGKAILGIGVGEREGNEPYGVDWSRPVARFEEALATIRVLWDSGGELVSRDSEYF
                     PLHKALFALPPYRGAYPPIWVAAHGPRMLHIAGRFADGWFPAIVLRPRDYAQRLDVVR
                     SAASDAGRDPSAILPAISLTVFTGRTRDAVDEAMSSVAAMSMALCLPGADWARHGGKH
                     PLGEDFSGAQDLIPQTLDEQSVLSYTAQVPPSLVREVTLNGTPEEIVDQVAVWRDHGV
                     RHVALTNLSGLQPSLQKALASNGSFTGLMRRLKKL"
     gene            complement(10603..10890)
                     /locus_tag="VFC01_18460"
     CDS             complement(10603..10890)
                     /locus_tag="VFC01_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81646.1"
                     /translation="MTSPDPPQKCFLTEWYQPALARRGIDDVVAALGEAATHLHHQGH
                     PIRLLVAVNAPTDQVLYSVFAAESADAVTQACLRAGCPADRITSGIQTRIP"
     gene            complement(10901..13699)
                     /locus_tag="VFC01_18465"
     CDS             complement(10901..13699)
                     /locus_tag="VFC01_18465"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN81647.1"
                     /translation="MAGGVVEREVEDRAVADFLTRAEGGPAGLVVEGEAGIGKTTLVL
                     EAADRAADRGFRVLSAHGSPAEVSYAYSAVADLVSGVDDATVVALPELQRIALQRAQS
                     GVVVADAPATDERIVANAFLSVIERMSAQAPVLLAIDDGQWLDASSRAVIGYTARRLT
                     GRTGLLVSFRTGESVSADAQLWLDFCRPETITRLRMRPLTLGGLHVLIAARLGHTPPR
                     PAIVRIYEISGGNPFFALEMAASTGTDVSATTVGLPENLAALVRRRIGEVDDDVAVVL
                     LAAACAAVPTVELVASATEMTLARVVESLESAESLGVVGIDGNQVRFTHPLFATGVYA
                     DAAPSRRRAMHRALAAVVDQPEVKARHMALAATTGEAATLAALDAAAEATSAQGAPAV
                     AAELIELALNLGGDTAQRRIRAGELHFRAGSLVPAREHLESTLADLSPGMLRCMASMW
                     LGAVKGYDDDTAGAVKAMSEAADGAGDNPPLRLLCLLRLALVLVMTDRLGEAIERGAM
                     AVEIADQLGVPELRSQALSIWVAGKFVYGLGVDHEALQTALALENPRGGATTWFRASA
                     VEAMISAYTGDLERARSQMLVLQQRMFDGGTEVDIIWAAVHLAAIAVWSGRYAEATEA
                     ARQAVERAEQMGGRLALTTAWTPQAAAAAYAGREAEARAAAKAAIDTAHEIGAPQLAK
                     EPTVTLGFLEVSLGNNAAALAVLQPRLDAFDPIHGTEIEGGAHLPDAVEALTAVGRID
                     DAEPLVDALERNGAARDRPWMLAVGARGRGQILAARGDLEAAQRAVEQALTHHERLPM
                     PFETARTHLLLGQLLRRRRQKQAAAATLSKALSTFENLGTPLWAQRARAELARLSVTR
                     AGNGLTAAEERVARRAAAGLSNKEIAADLFVSPKTVETNLSSVYRKLGIRSRAQLHAR
                     LNNGDARENPASRDAPPQ"
     gene            14221..21765
                     /locus_tag="VFC01_18470"
     CDS             14221..21765
                     /locus_tag="VFC01_18470"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZN81648.1"
                     /translation="MAKLTVNPVSAGRHRVERGGSRPCTVASGNTTYARYIGRVGALA
                     VALGVGLAVATGSGMGIARADESPSADSPSADSPSVKSEPTPETQSGGQPGDLNPSTP
                     PSAVSTPESTSGEPPSAPTNEPSVPEMNYDSSGGAQTSGDDADGETKTVSTPRSTTAS
                     GATQTPAARPKSPKKNDNSARASEPSVTAKADSSQTVSPGDATTTDTTAKPVTSATVV
                     GEPAEARVAPAPNGEIAMIDAQHTAAASTMQVSALSAPQRSTATPAVVEVNTSLLATA
                     LAPFAIPDPDAPTDAPALLTMLAWARRENQRTSVAEVPDVAPQETTLVLDTVSEPLAL
                     KTAAPAAKTQPAGSKPPRNTAPVATADRYTTTKGTPLTVTVPGVLANDTDAEANALTA
                     QLAGKPANGTVTLRTDGSFTYSPNANFIGTDTFSYKASDGTTTSKPTKVTITVTGAVN
                     RPPVAGNDSYSTNEDTPLTVATPGVLANDSDPDGNPITAQVVSNPSHGTLTLNSNGSF
                     TYNPAANYNGPDSFTYRTNDGATNSNTATVSLTINSVNDAPTLTLPPAGTLTPDANGG
                     VEFSVGGTDPEGSPITFSANTTQGSITQVTPTSFRYTPGDYAHLLAADGYSGPHTATI
                     TVTATDSAGGTTTSAFTVPVTPTNAGPTATGPAKTGTANPVNGAVPISFPVIDADQDT
                     LKFTDDTDLGDLAIANGQLVFVPTEAARHAAVADTATAADREVTFTLTANDGHGGTTS
                     TLVTVNIATSGVISSVTVPGNAAGGLRYTPDGTRAILTTQTAVYDAETQTYTTATRVA
                     VIDTATGKQVGTTTTMEGLPTNSYYEGYLPAMISADGTRASQVTSTTTYDSTTSTYTY
                     TTRVAIVDTATGTQVGDTITLAGSHNGTQYSADGTRLVQTTAGPTFDSATGTYTSTVR
                     VTVIDTATGTQVGPGTTLAGQYGSTQLRGDGTAKLTAQSSTYDSSTSTSTYTTRVAVI
                     DLRTGNQVGDVTTTTVSQSGSSGGSSLSTPDGTRIVRTTSERTYDQATYSYIDTTRVV
                     VIDTATGEQVGTTVTLAGPGSSSLSADGTRAAVTTSEQRYDQPTYSYVYTTRVAIIDT
                     ASGEQIGTTVTLNGSGATQFIADGTRAVVTTSSETTYDPYTYTHTYTTRVVVLDTANA
                     EQVGTTTTLTGSSTWLKVSADGTRAVATSLEETYDWIDGVNTYTSRAAIIDTAAGTQI
                     GTTTALRGYGYSNDVQLNADGTRALFITDMYDSTPGVYSHTSRVAVIDVTTGEQIGTA
                     ITVSGSSGQAQFFADGTRAILTSQEEIYVDGQRTYTYTTRVTVVDVTTGNQIGSTVTM
                     GGSPGGTLLNADETRLIQITTDRDEIFNGYTTRITMIDTATGDQVGTVTVRGEIDRYD
                     YNYTRNPAPFTPDGARLVVTTSDQYSGGAATRVVFVDVATGTQVGTATTLAGNANSNI
                     QFSADGTRAVQTTYAYTYANGSSTVLSTQVAVIDVATGTQVGPTTVLSGYSGGTVLSA
                     DGTRAFQTAYVYGNGTDSTTRLAVIDLATGNQVGVSAALPGYPTGTARLSADGTRVTL
                     TTISSAGTKVTVIDIDQAGSNERPTVAAETESNDPDANGTVTGAVYFIDPDSDTLSYA
                     GTGATLKGSVIVNADGSFVYTPTEAARLAAAKVSASPDAKQDTFTITAYDAHGGATPT
                     VVTVAVLPKNTAPVATSTPGDFSNGVLTGTINGTDVDGDPLTYSVATQGGKGTVAVNP
                     TTGAFTYTPSADARNAASATPNDVDTDTFTVTVDDGRGGSVTNTVTVDVPQTGVVADG
                     PVDLPGSGALQYSPDGSRAIRVTSVQVRVGDYYDYDYVDRTSIAVIDTATGAQIGETV
                     TIDGTSYNSALRYSADGAYASQIAVVSRGDYYNTVYSTRVAIIDTATGAVTVTELAGS
                     PSSTMTFTADGGYAIQTATASRYRYVNGSYTYTYASEVAIIDTATGDVIKVQLPSAVS
                     GFETDGSGALDATGTRAIFRTYRDGSNGALFVDTATGAVTEFSVPGYPYMQISDDHSR
                     LVVQSYQAAGTQYTVVDTATGLQVGSTFDFAAAQSAGYSSNGSQIVVLQQDSSSGTTV
                     TRLTAIDLTDGTQIGSTLTIAGAAYQIQYTDDGSRALVLGSGNGTVTVIDTSSFTTLG
                     TISNLGQYVSDVQFSADGHHAVLLGRAGDYYSGYTTKVAIVDTTTGAQLGATLDVAGS
                     AQAQFNSDGTRVMLTTSGTDTRVTVLDAATGALVGSTVTVTGSGQVLLNPAGTRATIT
                     ISTYSSGTASYTSRVAIVDLTTGTQLGSTIELGAAYTDSRFSPDGNVVALTTREGDYV
                     TGYTTHMTLVDVATGTQMGTTITQAGVGDVKFTADGNRVAVYTDTSGYGGQDKAITIT
                     VLDTSSGTQVGSTITVTGQSRGITSTPDRTRLLVTSFSDETGVTSLVVVDVTTGTQIG
                     ETVTLPGTAYYYNVTFNADGTRAVLNTYDNNNDNIHRVEVVDTVTGATQTYALRGEWQ
                     GSRFTSDGTRLIVDTLDNTAQGVAINRTSIIELT"
     gene            21955..22323
                     /locus_tag="VFC01_18475"
                     /pseudo
     CDS             21955..22323
                     /locus_tag="VFC01_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896546.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
     gene            22441..22914
                     /locus_tag="VFC01_18480"
     CDS             22441..22914
                     /locus_tag="VFC01_18480"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN81649.1"
                     /translation="MENIRAALTPAVEVDRFFVADRAGDIIGAIACTDHTGRAMRVDA
                     ADWRRRLGVVRGTVAARILASQWMSALDYPAATGYIEFVAVAERARRQGVATMLVEGA
                     IAQTPYTDFELEVTDVNSPARDCYCKIGFVDGRRKKEKFVRFKGFRERIYMRYSH"
BASE COUNT         4063 a         7892 c         7331 g         3633 t
ORIGIN      
        1 cagcgtcgag gcggccaccg cgaaagccag accgacgctg cgggacagct cgagtcccga
       61 cgtggccagc gacagtggga ttggaatcag cccgccgacc accgcggcga cccggaagtc
      121 acgcacccgg ccccgcagca cgtcggtcga caacgcgccg gcgatgctga ccagcagccc
      181 ggatgatgtc gcaaggaagg ccgcgatcgc gcccgcggcg accagtgccg cgagcagttg
      241 tccggccggc ccgccgatgg ccgcgccggg agcgagcagc accgcggcgt ccgctgtccc
      301 ggtgatcagt agctgcggca ctgacagccg ggcgaatgca cccaacagcg ttggaaacaa
      361 atagaacagc gacagcagcg cgatgactgc gagcgcggtg cgccgcgcag cacgtccgtc
      421 ggggttggtg tagaagcgca ccagcacatg cggtaaaccc atggtgccca agaaggttgc
      481 gacgatgatc gacacgacct ggtagagcgg atgaacgcca ccgagcccgc cgcctgacgc
      541 gatccacgcc ttaccggtgc tcggcgtgcc cgcgaccacg ggcgtcgccg ccccagcggt
      601 cagcgtcagc gtggtgcctg cgccgagcgt gtgttcgccc gctgacggaa tcggctgggc
      661 atcaacgtga ttgccgtcca gcatgccggt cactgtgatg cccgccggcg cggcgacctg
      721 taccaccacg tcggtttcga tcgcgacggt ggtgtgctgc tggaccgtcg gtggcattgg
      781 cccgccgagc tcgccccgct cggtgaagaa cagcccgagc aacgcgagtg cggggatcgc
      841 gatcgcggtc agcttcagcc agtactggaa ggcttggacg aacgtgatgg accgcatccc
      901 cccggcaacg acgttggcga tgacgatgcc gccgaccact agcgggccga tccaaacagg
      961 cacgccaaga agggttttca gcgccagccc agcgccctga tactgcggca ccaggtagaa
     1021 cacgcagatc acgacgacga caagcatcgc aactttgcgc agccgggccg agcccagccg
     1081 gaactccgcg aagtcgggca cggtgtaggc gcccgagcgg cgcagcggcg cggcgacgaa
     1141 cagcagcagg cccagatagc ctgcggtaaa gccgaccgga taccacagcg cgtcggcgcc
     1201 gtacttggcg atcagtccgg cgacgccgag aaatgaagcg gctgaaagat attccccgga
     1261 gattgcggcg gcgttccact gtgggccgac ggagcgcgag gcgacgagga aatcggatgt
     1321 gttgcgggac aaccgaaccc cgtacgcacc gatcgcgacg gtcgccaccg ccgctgcgag
     1381 caacgcggcg gccgtcaacg gtgacccgct catggttcgc tgtccaccac gccgacgaag
     1441 tcgcgctcgg cctgttcggc cagccgcacg tagacgcgcc cgatgccgta gagaagcgga
     1501 tacaccagca ccgcgagcac cagccagttc aaccgcatgc cgagcacggt cacggcgccc
     1561 aagtcgggaa agaccgcgtt gagcaacggg attgcgccga cggtgcatac caccaccgcc
     1621 gccaaccgca gcgccagtcc gagctgggcg cggaccaaac cgcgcaccag agcatcgcca
     1681 accagcgtct gctcctgcac ctcgacccgg gtgcggacca tgcgggcccc gcggcggtgc
     1741 gcgagcacga cgcgttcccg ttggggccgt tcaccgctgg tcatcgggcc tcaaattacg
     1801 catcggatcg cggaccagcc ggtcgcgaag ttcgcgggcc tgacgcctgc tgaccggaag
     1861 ttcgaccgca ggcgaggccc cgttcgcccg cagccgcacc agcaccgcgc cgtcgctggt
     1921 acgcagcccg gtgacgagcc gcagcgctac cagataggag cggtgtacgc gctggaaacc
     1981 tctgtcgcgc caccggcttt cgagcgtggt caacggaatc cgcaccagat gcgaacccga
     2041 tgcggagtgc agccgcgcgt agtcaccctc ggcctccacc cagccgatgg tgtcccgcgg
     2101 caccagatgc gtgacgccac cgagctcggc ggggatgaca tcgtcgtggc cgtcttcgga
     2161 ggggtcgggg cgcgcctcga caacccgtcg caccgcctcg tcgagccggt cctcgcgaat
     2221 cggcttgagc agataatcga tcgcgccgac gtcgaaggcg gcgatcgcct tgtcgtcgtg
     2281 agccgtcacg aatacgacca acggccggtg cgagaagttg gccagcacgc cggctagctc
     2341 gatgccggat aaccccggca tgttgatgtc gaggaagatc gcgtcgatgg tgcggctgtt
     2401 gagttctcgc agcgccgagg tggcgtcacc ggcgcgatac acctggccga tctcgggatg
     2461 ccggccgagc agataagcca gctcgtcgag cgcgggcgcc tcgtcgtcga cggcgagcac
     2521 agtgagcgcc ctgttcactc gacacctccg ctggcccgga cgcccgaccg gaacttgggc
     2581 acacgcatca cgaccttggt gcccgctccg accgccgtct cgaccaccag accgtagtcg
     2641 ttgccgaacg ccgcgcgcag ccgatggtcg acattcgtca ggcccacatg agcggcggcg
     2701 cccgagccgt cagctaaggc atcgccgggc ccggtgcgaa ggatttcggg atccattccg
     2761 acgccgtcat cttcgacggt gatcacgcag tcggcgccct cgtcgcgggc gacgatctcg
     2821 accgagcctt tgctctgtcc ggcgaaaccg tgccggacgg cgttctccac caacggctgc
     2881 agggccagga agggcacgac gacgttgagg acttccggcg cgacttgcag cctgaccttg
     2941 agcgcggtgc cgaagcgggc ccgctcgagg ttgaggtagc ggtcgatgtt gcgcagctcc
     3001 tcggccagcg tcgtgtactg gccggcggcg cggaacgagt atcgagtgaa gtcggcgaat
     3061 tcgaggatca gctcgcgggc ccggtcggga tcggtgcgca cgaacgaggc gatggtgttc
     3121 agcgcgttgt agatgaaatg cgggctgatt tgggccctca aggcgaggac ttcggcacgg
     3181 tcgaggcggg cacgcgacgc atcgagttgg gccagttcga tctggctggc ggcataacgg
     3241 gcgacctcgc cgaccgcgcc gagcatgccg gggccgggcg agcgcgtcgt caccacaacg
     3301 agcacaccga gaagctcacc gccctcttcc agcaacggct gggcgatgac ttgcgaagcc
     3361 tggccgtgcg tcaacacccg gcgctggccc gagatcgatt cgagcgcggc ccgctggcac
     3421 gcgtcgcggt ttgactcgtc gggcggatcc tgcgcgagca atcggccctc cccgtcgaac
     3481 agggccacgc cgtcggtgcc ggtgaggccg cgcaggaatg gcgcggcggt ctgggcggat
     3541 tcggtgtcca ggccgcggcg aagcgctcgg gcggccagcg aggcgatgtt cagcgcggcg
     3601 tgcactgcac gttcggttgg ggtcgcgacg acgcgacgag ttcgcaccgc gatgaccgcc
     3661 gcggccaccg ccgccaggat cagcgcggcg gccagtgcta tcgcgagctc gcccgacatc
     3721 tcacctcacc agggacctta gactgatcga gtggcgaaac tgcagctagt tcaagatccg
     3781 gcggccgatg cgctgctgga atccaatccc ttcgcgctgc tggtcggcat gctgctggat
     3841 cagcaatacc cgatggaggc ggcgttcgca ggccccaaga agatcgccga ccgcatcggc
     3901 ggggtggacc cccgcgaaat cgccgactac gacccggagg aattcgcggc gttgtgttcg
     3961 aaaacgcctg cgatacatcg ctttccgggg tcgatggcca agcgtatcca gaccctggcg
     4021 cagatcgtcg tcgaccagta cgacggtgag gccgcggcgc tgtggacggc cggtgccccc
     4081 gacggggcag aggtgctgcg gcggctcaag tcgctaccgg ggttcggcga gcagaaggcg
     4141 aagattttcc tggcgctgct gggcaagcag tacggcgtga cgccgaaggg ctggcgcgaa
     4201 gcggcgggaa attacggcac ggccgggtcg tatctgtcgg tggccgatgt gacggacgct
     4261 gggtcgctgg agaaggtgcg gtcgtacaag aagcaggcga aggcagcagc gaaggccgcc
     4321 aagggctagc ttgtctggcg ctcgtgctcc aggtcgagct tggccaatga cgtgatgacg
     4381 ctggctacga cgcgtccggt cgatgtgccg gtgtccaggc cttccttcag cgcctgcagc
     4441 cggattcccc ggtcggtgag ctcggcgatc gttcgtgcca cttcggcggc cgaccgaccg
     4501 agcctgtcga tcgcgacgac gatcaccgtg tcgcccgaac gggcgtagct cagcatcgcg
     4561 tgcaaacccg cacgcacccg ctcggcggag tcggacaact tgtccgtgaa gacccgccgc
     4621 gggtcgacgc ccgccgcgtt gagcgccgcg agctgtccct caaggttctg cccggcggtg
     4681 cttgctgtgg cgtagcccag cgtgcaggtc acgaacccaa ggtctcatga cagccgccat
     4741 ttgcggaaga gacgacgtct tatcgagtct ggctaactgc agaactgtta tcgaatggtt
     4801 cgccgtcgaa ccgctcgcgg gtcgcgagtt ccgcgacggg cttgcgggtg agctttctga
     4861 gttggcggac cggcttgcca agcggcagca gggcggcgac ggcgaagtcc tcggggatgc
     4921 cgagcagttc tttgagccgc ggttcctcgg cgaccgctgc cgtggtcagc acgccgccgt
     4981 agccctcatt gcgggcggcc agcaggatgt tccagacgaa cgggtacacc gatgcgcctg
     5041 ggatgacgcc gatccggtcg aggtcttgat cgaatgccgc aacgacggcg aggttgacgc
     5101 agacgaccaa cacgacgggc gcgtcgcgca gcggcgaccc gggcggcacc tcgacggcgg
     5161 cgatcgtttc cgcgtcgact ccgcacggct gcaacggatt ccatggcccc tcgccattgc
     5221 ggagctgagc gatgtagcgg cgggcaccgg ggatggccag gtcggcgagt cgatcgcgcg
     5281 tctgctgatc gcggacgacg accacgtgtg cgccctgccg attgccgcca ctgggtgcga
     5341 accgcgcgtt gtcgaggatg cgttcgagca cctcgtcggg cagcggatcg tcggtgtatt
     5401 cgcggaccgc acccgtggtg cgcatgactt cggatagctc cacgacggcc atcatgcttg
     5461 atatgcgatc gtgaacatat gaagacgcat ctgaactgcc cttgcggcga agcgatcacc
     5521 ggcaaggacg aggacgacct cgtcgagaag gcccaggctc acctgtccga ggcgcatccc
     5581 ggccgcgagt acgaccgcga cgcgatcctc ttcatggcgt actgatccgc cgcgcgagcg
     5641 cacgcttgtt gcacacaaaa cgcgagaagg tgcattaaca accgtgcgct cggcgcaatg
     5701 agcgctacgg cacgaccgtt gagccgattg tcggcatgaa ctggcactgc ttctcggtcg
     5761 tggtgacttg gccgaagatc gttgacatga tgctgcccga gccggtgtcg acgatcgccg
     5821 tcagcgtcgt cggcccgttg ggattgatgt ccggccgcgg cttcagcgcc gcgctgcccg
     5881 acttgccggt ggtcaggttg acccatgtga cgttcaacgg cagcttctgc tcggccgctg
     5941 gcgcgggcgt gccgaccgcc gtgaacacgt acgccgtctg accgggccct ggcccgggtg
     6001 ctgggatcct cgccgggcct gcaaccgaaa tcgcggtcgc cagcacgttg ccgccgtccg
     6061 ccaagcaccc gttgctgatc gatgggtaca ggaagtcctg cgtcggcggc gcgtcgggac
     6121 caaacgcagg cgcggccgcc ggtgctgcag cgggatcagg cgccgatgca ggggcagggg
     6181 caggggcagg ggctggggcg ggtgccgctt cgggagccgg cgcgaccacc ggggccacct
     6241 ccggtacagg ccccactgcg tgcgccgggt tgacgcccgc gggcagatgc gcctcttcgc
     6301 caggtaccgc gccaggcgcc ggaacatgcg cgacgaactg attcaccgcc gtcgcgacat
     6361 tctgcgaatc cgcaggcatc gccgaattgc cggcgaacac cgcggcggcg gccatcagcg
     6421 tcgaggcagc gttcgtcgga tcggcagcgg cctgctgaat ggccgggccg atgctttgca
     6481 ccgcagtcaa acccggcgct tgcagcgcgt cgatcggcaa cggtggcgcc ggttccgcat
     6541 tggctaccgg cgtcatccca cccagcagta atgccgtcga cgccactacg gcgccggcca
     6601 atgtcgtcct gaccgatctc atgaagtccc cgattcctgg ttgtggttgt cgctgcgcgc
     6661 ccgccgtcac ggcagggcgg acagcggcat catcatcggc gcgagaggtg cggtggacgc
     6721 cgccgcggga gcggctgcgg cgggtgcggc cgctgcggcc ggcgcggtcg ccgctgccgg
     6781 cagcagaccg gccagcggtg taccggcggg catcaacgac gccaacgcac tcgggaccga
     6841 caaaccctgt gtcagcagat tctgcggcgc ggtcgcgggt gccgctgcgg cagcggccgg
     6901 gacggcggcg ggagccggag gctgcggaag actgaccgat gcggttgcgg ccggcgcggg
     6961 tgcggtcgcg gcagccgggg ccgcagcggg ggccgctgac tgcacaccgc tgagcaccga
     7021 cgtaaggccc tgcatgaggg tcgtcgcggc ggccggattg gtggcgagct gctggaggaa
     7081 cggcaggccc ggcacgccgg gggtcggtgc cggcgccgcc ggctcggctg cggccgtcgc
     7141 gctgagcccc agcgcgatcg gaatcgcacc cgcggctgcg atcatgctgg tggcaagcat
     7201 tttcctggtg aatttcatgg ttcttccaat ccgttgaagg cacgcctgcg cccgagagtc
     7261 gtccggttac tggtgttact caagtgacac gtgtggcgga gatctaaacg ttacggatgc
     7321 gacggcttac cgtgatccct cgctagagtc ggcgggatag acgcctcgac tgccgccgct
     7381 ccaaccgctc cgcgtatgcc cgcccgtctg gctgccgcgg cgccggtgct cttggcgatc
     7441 agcatcgccg cgcgacttgc gatgacctat ctagtgccgc acggcgccaa cttcgtcgac
     7501 ttgcacgtct atgtcggcgg cgcggagatg ctcgagcaac atggcaagct ctacgactac
     7561 gtctacgccg atcagacgcc tgacttcccg ctgccgttca cctatccgcc cttcgcggcc
     7621 gtcgtgttct acccgcttca cttcatccca ttcgggatcc tgacgttcgc ttggcaagtc
     7681 gcgatcatcg cctcgctcta cggcgtggtg cgaatcagcc aacgactgct gtcgggggag
     7741 ggcgatcggc gcatcgcaat gctgtggacc gcgctcggca tttggctcga accgatgcgg
     7801 agcaatttcg actacgggca gatcaacgtg atgctcgtgt tggccgtgtt gtacgcggtt
     7861 tactgcacgc ggtggtggtt gtccgggctg ttggtcgggc ttgccgcagg ggtgaagctg
     7921 acgcctgcgg tgtcgggctt gtacttcgtc ggcgcgcgac ggtgggccac ggcggtgttc
     7981 tcggcggtgg cgttcttcgg caccatcgcc gtgtcggcat tagtcgtcgg cgaccaaacc
     8041 cgttactact tcaccgaatt gctcggtgac gcccgtcgtg tgggaccgat cggaacgtcg
     8101 ttcaaccagt cgtggcgcgg aggtatctcg cggattctcg ggtacgacgc gggctacggg
     8161 ccgctggtgg tagcggccat cgtggtgacc gccttgctgg cgatactggc ctggcgagcc
     8221 attggcggcg ccgatgatcg gctgggcgcg atcgtggtcg tgcaactctt cggtctgctg
     8281 tcgtcaccga tctcgtggac ccatcactgg gtgtggctga tcccgctgat gatctggctg
     8341 ctgcacggcc cgctgcgtga gcggctgggc gcgcgggtgc tgggctgggg ctggctggcc
     8401 ctgaccatag tcggggtgcc gtggctgctg agcttcgcgc agccgacgat ctgggtgatt
     8461 ccccggccgt ggtatctggc ctgggcaggg ttgatctaca tcgtcgcgac gctcgcgacg
     8521 ctggcgtgga tcgccgctag ccgacgatca ggtttatctc gtgcgccatg tccagatcgt
     8581 tctgggtgat cccgcccgcg gcgtgtgtga ccaacgcgaa agtcactgtc cgccaacgaa
     8641 tatcgatgtc ggggtgatga tccttctcct cggccctcgc ggctacgcgg cgcaccgcct
     8701 cgatgccgtc catgaacgaa ggaaacttga tcgagcggcg aatcgcgccc tgggaccgct
     8761 gccagccgtc gaggtcgggc agtgcggcgt ctacttggtc gtccgttaac acagccatac
     8821 ttccgaccgt accgctgttg gctctttagc cgaatccatc gcagccgacg acggttgtac
     8881 cgcgctccag cccgaccgtt gctgtcggac ccacaccact gccggccgcc acgagcgcgc
     8941 aggctcctcc agtcaacgcc tccgcggtgc tgacagagga atgtcgccta tggcagagcg
     9001 tgattgcggt tgtagcgact aaggctctgg cgaatcctcg gaggcgtact ccctcctgat
     9061 tgacgcagcc gttcggggaa catgagttgc tgtgccgctt tacgcttttc atgccgcagc
     9121 caataccttg gcgtcttggt gcacagctcg ccgatacggt cctgctgccg tagggaagcg
     9181 gctcgagtgc tggggggatg gggcagtggc tttaagtgtc gggttcgtcg agcaccccgt
     9241 gataacgcgc ggctggccga ctgctgccgt acgcagcggc tatctcaggg cagcacttgc
     9301 cggagtcgac acctattgga tgcctgacca cttgaacagc ctcgtgccac gatccattgc
     9361 agttccgaag tacgttggcc ttgcccgggt tgtgcccgac gttgatgcgg tttgtgaacc
     9421 gtggacgttg ttgggccagg tcgccggctg gaaccgcatc ggacggctgc gcttaggggt
     9481 tgcggtgaca gacggcggcc gtcgcaaccc tgcggtcacg gcgcaggcgg cggcaacgtt
     9541 gcacctgatg agtcgaggaa aggcgatcct cggcatcggt gttggggaac gcgagggtaa
     9601 cgaaccttac ggtgtcgact ggagtagacc ggtcgcacga ttcgaagagg cgctggcgac
     9661 gatccgagtc ctgtgggatt ccggcggcga attggtgtcg cgcgattcgg agtactttcc
     9721 tcttcacaaa gcgctcttcg cgctgccgcc ctatcgcggt gcgtacccgc cgatctgggt
     9781 cgccgcacac gggccaagga tgctgcacat cgctggtcgt ttcgctgatg gatggttccc
     9841 cgcgatcgtg ctgcgcccca gggactatgc gcaacgactt gatgtggtgc gttcggcggc
     9901 ctcggatgcc gggcgggatc cttccgcgat cctgccggcc atctcgttga cggtcttcac
     9961 cgggcgcacc cgcgacgcgg tcgatgaggc gatgagctca gtcgcggcga tgtctatggc
    10021 gttgtgcctc cccggtgcgg actgggcccg ccatggcggg aagcacccac tgggcgagga
    10081 tttctcgggc gcacaagacc tgattccgca gacgctcgat gagcagtctg tgctgtccta
    10141 cacagcacag gttccgcctt cgcttgtcag agaagtcacg ctcaacggga cacctgagga
    10201 gatcgtcgat caggtcgcgg tgtggcgcga ccacggggtc cggcatgtgg ccctgacaaa
    10261 cctcagtggg ctgcagccca gcttgcagaa ggcgctggca tcgaacgggt ctttcactgg
    10321 tctcatgcgc aggctgaaga agctgtgaga tgtccgatca gatgacaacg gtcgccgcta
    10381 caaatccgcc gcatctcggt ggcgcgttgc taccgtgtgg ctttcgcgca tgacagaacc
    10441 gggttcgcga agtgaggctt tctatttcgc gtgtttttcg acgggctcac cgttttggtg
    10501 cctactggcg cgtagtcctg aactcagcgt cgcgtcgggt cgttctccga tccgcgacca
    10561 cgcggttgtc agcgcgtcgg ggcaggctcc cgtcaaattc tgttagggga tgcgtgtttg
    10621 gatgccgctg gtgatgcggt cagcgggaca gccggcgcgt aggcacgctt gcgtgacggc
    10681 gtcggcagat tcggcggcga agacgctgta gagcacttga tcggtgggcg cgttcaccgc
    10741 gacaagcagc cgtatcgggt gcccttggtg atgcaggtgc gtggctgcct cgccgagggc
    10801 cgcgacgaca tcgtcgatgc cccgacgagc cagcgcaggc tggtaccact ccgtgaggaa
    10861 gcacttctgc ggcgggtcag gactggtcac tgtttggacg ttactgcggc ggcgcgtcgc
    10921 gcgaagcagg gttttccctg gcatcgccgt tgttgagccg ggcatgaagt tgcgcacggg
    10981 aacgaatgcc gagtttgcgg tagacgctgc tgaggttcgt ctcgacggtc ttgggtgaca
    11041 cgaacaagtc ggcggcgatc tccttattgg acaacccggc cgccgcgcgc cgcgcgacgc
    11101 gttcctcggc agcggtcaag ccattgccgg cgcgtgtgac gctgagccgg gcgagttcag
    11161 cgcgggcccg ctgggcccac agtggcgtcc cgaggttctc gaaggtgctc agcgccttcg
    11221 acagcgtcgc cgccgccgcc tgcttctggc ggcgacggcg tagcagctgc ccaagcagca
    11281 aatgggtgcg ggccgtctcg aacggcattg gcaaccgctc atggtgtgtc aaggcctgct
    11341 ccaccgcgcg ctgagcggcc tcgagatccc ctcgcgcggc caggatctgg ccgcgtcccc
    11401 gcgcccctac cgccagcatc cacggccgat cacgggcggc gccgttgcgt tcgagggcat
    11461 cgacaagcgg ttcggcatcg tcgatgcgtc ccaccgcggt cagcgcctcg accgcgtccg
    11521 gtaggtgagc accgccctcg atctcggtgc cgtgaatcgg atcgaacgcg tcgagccgcg
    11581 gctgcagcac cgccagcgcc gcggcattgt tgcccaggga aacctccagg aatcccaaag
    11641 tcacggtggg ctctttggcc aactgcggtg cgccgatttc gtgggcagtg tcgatcgctg
    11701 ccttcgccgc tgcgcgcgct tccgcctccc gacctgcata cgccgccgcg gcggcttggg
    11761 gtgtccatgc cgtagtcagc gccagccgcc cgcccatctg ttcagcccgc tcaacggctt
    11821 gccgtgcggc ctcggtcgcc tcggcgtaac ggcctgacca gaccgcgata gcggcgaggt
    11881 gcaccgcagc ccagatgatg tcgacctcag tgccgccgtc gaacatgcgt tgctgcagta
    11941 ccagcatctg gctgcgggcc cgctcgaggt cgccggtata ggcagagatc atcgcctcga
    12001 cggcgctggc ccgaaaccac gtagtggcac cgccgcgagg attttcgagc gccagggctg
    12061 tctgcagtgc ttcatgatcg acaccaaggc cgtagacgaa cttgcctgcg acccatatcg
    12121 acagcgcctg gctgcgcagt tctggtacgc cgagttgatc ggcgatctcg acggccatgg
    12181 cgccgcgctc tatggcctca ccgagccggt cagtcatcac caggaccaat gccaggcgta
    12241 gcaggcacag caaccgcagg gggggattgt cgccagcccc gtcggcggcc tcggacatcg
    12301 ccttgacagc gccggcggtg tcgtcgtcgt accccttcac ggcgccgagc cacatcgacg
    12361 ccatacagcg caacattccc ggcgagaggt cggcgagagt cgactctagg tgctcgcgcg
    12421 ccggcaccag tgagcctgcg cggaagtgca gctccccggc gcggatccgg cgctgcgcgg
    12481 tgtcgccacc gaggttgagc gccaactcga ttagctccgc tgccacagcc ggagcaccct
    12541 gggcggaggt ggcttcggcg gccgcgtcca aagccgccag cgtggccgcc tcgcctgtcg
    12601 ttgccgccaa cgccatatgc cgcgccttga cctccggctg gtcgacgacc gcggcgagcg
    12661 cgcggtgcat ggcccgccgc cgcgaaggcg cggcgtcggc atacacaccg gtggcgaaca
    12721 gcgggtgggt gaaacgcacc tggttaccgt cgatgccgac aaccccgagg ctttctgccg
    12781 attcgagcga ctcgacaacc cgtgccaaag tcatttccgt cgcggaggcc accagctcta
    12841 ctgtcggcac ggccgcgcac gccgctgcca gcaacacgac ggcgacatcg tcatccacct
    12901 cgccgattct gcggcgcacc agcgcggcca gattctcggg caacccgact gtggtggccg
    12961 acacgtcagt gcccgtgctg gcggccattt caagcgcgaa aaacgggttc ccgccggaga
    13021 tctcatagat ccgcacgatc gccggtcggg gcggtgtgtg ccctaaacgc gctgcgatca
    13081 agacgtgcag gccacccaag gtcagcggac gcatccgcag ccgcgtgatc gtctcaggtc
    13141 ggcagaaatc aagccacaac tgcgcgtcag ccgaaaccga ctctcccgtt cgaaagctca
    13201 ccagcaaccc ggtgcggccc gtcaaccggc gggccgtgta gccgatcacc gcccggctgg
    13261 aggcgtcgag ccactgaccg tcgtcgatcg ccaacagcac cggtgcctgc gcgctcatcc
    13321 gctcgatcac cgacaggaac gcgttggcga ctatgcgctc atcggtggcc ggtgcgtcgg
    13381 cgaccacgac gcccgactgc gcccgctgca gtgcgatgcg ctgcagctcc ggcaacgcga
    13441 ccaccgtcgc gtcgtccact ccgctcacca agtcggccac cgccgagtac gcatagctca
    13501 cctccgcagg cgagccgtgc gccgacagca cccgaaaccc gcgatcggcg gcgcgatcgg
    13561 cggcctcgag caccaacgtg gtcttgccga tgccagcctc accctcgacc accagacccg
    13621 ccgggccccc ctccgcgcgg gtgaggaaat ccgcgacggc gcggtcctcc acctctcgct
    13681 cgactacgcc ccctgccacg ctcagtccct tccggccacg acgaaaatgt cgccgctgac
    13741 gctgattggc ttcgtccacc cagcgactgg cacaaccccg aaatctctac tgcacatctc
    13801 gcgatccgat cctttgccgg ctgagccgca aaggccttcc gctggcggga cgcgcccgca
    13861 acggtctgca gccccggtgc gccaacacgt cccggccaca cccagcatca tcgcagccac
    13921 ttatcagcaa aggagaacaa acgcagcact gtgccatcca gaccgaggcc cgacatatgt
    13981 agcgcgggct gagctactcg ggctgcggtg cgatctggcg gtacatcgcg gtagctgaga
    14041 agccaacgcg gtagatcttg cattggtttc aaactcaacc aaatttgcct aggttcgtta
    14101 attcaatgca atgggggcga agcaaaagtc cagggaaacc cctgattccc ccgcgcgcag
    14161 ctgtgcatag cgtccttcac aggttctttg cagcaatcgt ccagggcaag ggggctcgtc
    14221 atggccaaac tcactgtgaa tccggtatct gctggtcgac atcgtgtcga gcggggcggc
    14281 tcgcggccgt gcacagtcgc tagcggcaat accacctatg cccgctatat cggccgcgtc
    14341 ggcgcgctgg cggtggcgct gggagttgga ctggcggtgg ccaccggctc cggaatgggt
    14401 atcgcgcgcg ccgatgaatc gccgtcggct gattcgccgt cggctgattc gccgtcggtg
    14461 aagtcggagc caacgccgga gactcagtcg gggggtcagc cgggtgatct caacccgtct
    14521 accccaccgt ccgctgtgtc aacgcccgag agtacctctg gcgagccgcc gtcggcgccg
    14581 acgaacgaac catcggtccc cgaaatgaac tacgacagtt ccggtggcgc ccagacgtct
    14641 ggcgatgacg ccgacggcga gacgaagact gtgtcaacac cgcgatcgac gaccgcttcg
    14701 ggcgctaccc aaacgccagc tgcgcgcccg aaatccccga agaagaacga caattcggcg
    14761 cgagccagcg agccatcggt caccgccaag gcggattcgt cccaaacggt gtcacccggc
    14821 gatgcaacca cgacggacac aacggcgaag ccggtcacat cagcgacggt cgttggcgag
    14881 cccgcggagg cccgtgtggc ccccgcgcca aacggtgaga tcgcaatgat cgatgcgcag
    14941 catacagccg cggccagcac gatgcaggtg tctgcgctca gtgcgccgca gagatccacc
    15001 gcgacgccag ccgttgtcga ggtcaatacc agtctgctcg caacggcgtt ggcgcccttc
    15061 gcaattcccg atccagatgc tccgaccgac gcgcctgcgc tgttgacgat gctggcctgg
    15121 gcacgccgcg agaaccaacg cacctccgtt gccgaagttc ctgacgtagc gccgcaagag
    15181 acgacgctgg tactcgacac ggtcagcgag ccgcttgcgc tgaaaaccgc cgccccggcg
    15241 gcgaagacgc agcccgccgg cagcaaaccg ccgaggaaca cggcgcccgt cgccaccgcc
    15301 gaccgctaca ccaccaccaa aggcactccg cttactgtca ctgtcccggg tgtgctggct
    15361 aacgacacag atgccgaggc caatgccctg accgcgcagt tggctggcaa gcccgccaac
    15421 gggactgtca cgcttcgaac cgacggctcg ttcacctaca gtccgaacgc caacttcatc
    15481 ggcactgaca cgtttagcta caaagccagc gacggcacga ctacaagcaa gcccaccaag
    15541 gtgaccatca ccgtcaccgg tgcggtcaac cgaccgccag tcgcgggcaa cgacagctac
    15601 agcaccaacg aagacactcc gctaaccgtc gccactccgg gagtgctggc caacgacagc
    15661 gatccggacg gcaatccgat taccgcacaa gtcgtttcga acccatccca cggcaccctc
    15721 acactgaact ccaacggctc attcacctac aacccggctg cgaattacaa cgggccagac
    15781 agtttcacct accgcactaa tgacggtgca accaatagca acaccgcgac ggtgagcctc
    15841 accatcaact ccgtcaacga cgccccaacc ctgacgctgc cgccagcggg cactctaact
    15901 ccggacgcca acggtggcgt cgagttctct gtgggtggaa ccgatcccga aggttcccca
    15961 atcacgttca gcgccaacac cactcagggt tcgatcaccc aggtgacgcc gactagcttc
    16021 cgctacacgc ccggcgacta tgcgcacttg ctggccgccg acggctactc agggccgcac
    16081 accgcgacga tcaccgtcac ggccaccgac agcgccggcg gcaccacgac atcggcgttc
    16141 accgtgccgg tgacacccac caacgcgggg ccaacagcga ctggtccggc gaagaccggc
    16201 acggcgaacc cagtcaacgg tgctgtgccc atctccttcc cggtgatcga cgccgaccag
    16261 gacacgttga agttcaccga cgacaccgac ttaggtgact tggcgattgc caacggccag
    16321 ttggtattcg tccctaccga ggccgcccgt catgccgcgg tcgccgacac agccaccgca
    16381 gcggaccggg aagtcacgtt caccctcacc gccaacgacg gccacggcgg gacgacctcg
    16441 acgttggtta cggtcaacat cgccacctcc ggcgtcatca gctcggtgac ggtcccaggc
    16501 aatgcggctg gcgggttgcg atacacgccc gacggcaccc gcgccatcct gaccacccag
    16561 accgcggtct acgacgccga gacccagacc tataccaccg ccacccgcgt ggcggtcatc
    16621 gacacggcca ccggcaagca ggtcggcacc accaccacca tggaggggct tcccaccaac
    16681 agctactacg aaggttattt accggcgatg atcagcgcgg acggaacccg cgcctcccag
    16741 gtcacgtcga ctacgaccta cgacagcacg accagcacgt acacctacac cacccgggtg
    16801 gccatcgtcg acactgccac cggtactcag gtcggggaca cgatcaccct ggccggctcg
    16861 cacaatggga ctcaatacag tgccgacggc acccggctgg tccaaaccac cgctggtccg
    16921 accttcgaca gcgccactgg cacatacacc tccaccgtcc gggtgacggt catcgacacc
    16981 gccaccggga cccaggtcgg ccccggcacc accctggccg gccagtacgg ctcgacgcag
    17041 ttgaggggcg acgggacggc caagctcact gcgcagagtt cgacctacga cagctccacc
    17101 agcacatcca cctacaccac ccgagtggcc gtgatcgacc tcaggaccgg aaaccaggtc
    17161 ggagacgtca ccaccaccac cgtctcgcag tccggctcgt ccggtggatc gtcgttgtcc
    17221 acccccgatg gcacccgcat cgttcggacc acgagtgagc ggacgtacga ccaagcgacc
    17281 tactcgtaca tcgacaccac gcgtgtcgtc gtcatcgaca ctgccaccgg cgagcaggtc
    17341 ggcacgaccg tcacgttggc cggcccgggc tcatcctcgc tcagtgctga cggcacccgc
    17401 gccgcggtga ccacgagtga acagaggtac gaccaaccca cttactccta cgtctacacc
    17461 acgcgggtgg ccatcatcga caccgccagt ggtgagcaga tcggcactac ggtcacgctg
    17521 aacggctccg gcgcaactca attcattgcc gacggcaccc gcgccgtcgt caccaccagc
    17581 agcgagacga cgtacgaccc ctacacgtac acccatacct acaccacgcg tgttgtggtc
    17641 ctcgacaccg ccaacgccga gcaggtcggt acgaccacca cactgacagg ctcgtccacc
    17701 tggctgaagg tcagtgccga tggcacgcgg gccgtcgcca cctcgctgga ggagacgtac
    17761 gactggatcg acggcgtcaa cacctacacc tctcgggcgg ccatcatcga caccgcggcc
    17821 ggcacccaga tcggcaccac cacggcgctg aggggctacg gctactccaa cgatgtgcag
    17881 ctgaacgccg acggtacccg tgcgctgttc attaccgaca tgtacgacag cacgccgggc
    17941 gtctactccc acacctctcg ggtggccgtc attgacgtca ccaccggtga acagatcggc
    18001 accgcaatca cggtgtccgg cagttccggg caagcgcagt tcttcgccga cggcacccgc
    18061 gccatcctca cctcacagga agagatctat gtcgacggcc agcgcaccta cacctacacc
    18121 accagggtca cggtggtcga cgtcacgacg ggcaaccaga tcgggtccac cgtcacgatg
    18181 ggcggctctc ccggcgggac gcttctcaac gccgacgaaa cccgcctcat ccagatcaca
    18241 accgaccgtg acgagatctt caacggttac accactcgga tcaccatgat cgacactgcc
    18301 accggcgacc aggtcggcac agttaccgtg cgcggcgaga tcgaccgcta cgactacaac
    18361 tacacccgca acccggcgcc attcaccccc gacggcgctc gcctggtcgt aaccaccagc
    18421 gaccagtaca gcggcggcgc ggcgacccgg gtggtcttcg tcgacgtcgc caccggtacc
    18481 caggtcggta ccgccaccac cctggccggc aacgccaaca gcaacatcca gttcagcgcc
    18541 gacggcaccc gggccgtcca gaccacgtac gcctacacct acgccaacgg cagctccact
    18601 gttttgtcca cccaggtggc tgtcatcgac gtcgccaccg gcacgcaggt cggccccacc
    18661 accgtcttga gcggttactc cggcgggacg gttttgagcg cggacggtac ccgcgcgttc
    18721 cagaccgcct atgtctacgg caacggcacc gattcgacca cccggttagc cgtcatcgat
    18781 ctagcgaccg gcaaccaggt cggcgtctca gcagcgctgc cgggctaccc gacgggaacc
    18841 gcgaggctca gtgccgacgg cacccgcgtc accctgacca cgatctcgtc ggcaggcacg
    18901 aaggtaaccg tcatcgacat cgatcaggcg ggctccaacg aacgcccgac cgtcgccgcc
    18961 gaaactgagt cgaacgaccc cgacgccaac ggcaccgtca ccggggcggt gtacttcatc
    19021 gatcccgaca gcgacacgct gagctacgcc ggcaccggcg ccacgctcaa gggcagcgtc
    19081 atcgtcaacg ccgacggcag cttcgtctac acacccaccg aggccgcgcg actcgcggcc
    19141 gccaaggtct ccgcctcccc cgacgcgaag caggacacct tcactatcac cgcctacgac
    19201 gcacacggtg gggcaacacc cacggtggtc acggtggcgg tgctgccgaa gaacaccgcg
    19261 cccgtggcga cctcgacgcc gggcgacttt tcaaacgggg tgttgaccgg cacgatcaac
    19321 ggcactgacg tcgacggcga cccgctgact tattcggtgg cgacgcaggg cggcaaggga
    19381 acggtcgcgg tgaacccgac cacgggcgca ttcacctaca caccgagcgc cgacgcccgt
    19441 aacgccgcgt cggccacccc gaacgacgtc gacaccgaca ccttcaccgt caccgtcgac
    19501 gacggccgcg gaggcagcgt caccaacacc gtcaccgtcg acgtgccgca gactggggtg
    19561 gtcgccgacg ggcccgtcga cctgccgggg tccggcgcgt tgcagtacag ccccgatggc
    19621 agccgcgcca tccgcgtcac cagcgtccag gtccgtgtcg gcgactacta cgactacgac
    19681 tatgtggacc ggacgtcgat cgcggtcatc gatacggcca ccggcgctca gatcggcgag
    19741 accgtcacca tcgatggcac gagctacaac agcgccctgc gatacagcgc cgatggcgcg
    19801 tacgccagcc agatcgccgt ggtgtcgagg ggcgactact acaacaccgt atacagcacc
    19861 cgtgtggcca tcatcgacac cgcgaccggc gccgtcaccg tcaccgagct ggccggctcg
    19921 ccgtcatcaa cgatgacgtt cactgccgac ggcgggtacg ccattcagac cgcgaccgcc
    19981 tcgcgctatc gctacgtgaa cggcagctac acctacacgt acgcatccga ggtcgcgatc
    20041 atcgacaccg ccaccggcga cgttattaaa gttcaactgc cctccgcagt gtcgggtttc
    20101 gagaccgatg gcagtggagc cctcgatgcc acgggaaccc gtgcgatttt ccgaacctat
    20161 cgcgatggct cgaacggcgc actcttcgtc gataccgcga ccggcgccgt cacggagttc
    20221 tcggttccgg gctacccgta catgcaaatc tccgatgacc actcgcgcct ggtcgtccag
    20281 tcctaccagg cggcgggcac tcaatacacc gtcgtcgaca ccgccactgg cttgcaagtg
    20341 ggctcaacct tcgacttcgc cgccgctcag tccgccgggt actcctccaa cggaagccag
    20401 atcgttgtct tgcaacaaga ttcctcaagc gggacgaccg tgacccggct gacggcgatc
    20461 gacctcaccg acgggaccca gatcggcagc acactgacga ttgcaggcgc ggcctatcaa
    20521 atccagtaca ccgacgatgg cagccgggcc ctcgtcttgg ggagtggcaa cggcaccgtt
    20581 acggtcatcg acacgtcgtc gttcaccacg ctcggcacca tctccaacct cggccagtac
    20641 gtcagcgacg tccagttcag cgccgacgga catcacgccg tcctcctggg ccgcgccgga
    20701 gactattact ccggctacac cacgaaagtg gcgatcgtgg acaccaccac cggcgcgcag
    20761 ctcggcgcca ccctcgacgt ggcgggctcc gcccaggcgc aattcaactc cgatggaacg
    20821 cgggtcatgc tcaccaccag cggcacggac acccgcgtca cagtgctcga cgccgcgacc
    20881 ggcgccctgg tgggcagcac cgtcaccgtc actggatcgg gtcaagtgct cctcaacccc
    20941 gccggcaccc gcgcgacgat caccatcagt acgtacagct caggcacggc tagctacacc
    21001 tcccgggttg cgatcgtcga cctcaccacg ggtacccagc tcgggagcac gatcgagctg
    21061 ggtgccgctt acacagattc gaggttcagt cccgacggca acgtggtcgc attgaccaca
    21121 cgtgaaggcg actatgtgac cggctacacc actcacatga ccttggtgga cgtggcgacc
    21181 ggcacccaga tgggaaccac gatcacccag gccggcgtcg gagacgtcaa attcaccgcc
    21241 gacggcaacc gcgtcgccgt ctataccgat acgtccggct acggcggcca agataaagcg
    21301 atcacgatca ccgtgctcga cacgagttcc ggaacgcaag tgggaagtac catcaccgtc
    21361 accggtcaga gtcgggggat cacctcgacc cccgaccgca cgcgcttgct cgtaacgagc
    21421 ttcagtgacg agaccggagt caccagcctg gtggtcgtag acgtcaccac cgggacacag
    21481 atcggcgaaa cggtcacgct gccggggact gcctactact acaatgtcac gttcaacgcc
    21541 gatggcaccc gcgccgtgct gaacacgtac gacaacaaca acgacaacat ccatcgggtg
    21601 gaggtcgtgg acacggtcac cggcgcaaca cagacctacg ccctgcgcgg agagtggcaa
    21661 ggctcccgct tcacgtccga cggcacgcgg ctcatcgtcg acaccctcga caacaccgcc
    21721 caaggtgtgg ccatcaaccg gacatcgatc atcgagctca cctaatcccc accctgcatg
    21781 ttcggcgact acatcgcccc ggggcgctga tggttcagcg gtggtgccga tctacggctc
    21841 ggatacaacg aaactcgttc gtatcgacac agtccacacg tacgtgccgc cgctccaacc
    21901 ttcacatgac gcggtatccc gtctttcagt cccggcggta tacggtccgt atcgatgcca
    21961 agtcagatcg tcgttgccgg tgcgctgatc tcgggttccc tgctattggt ggcgcaacgg
    22021 gcccggccgc cggaactcgc cggcctctgg gagctgcccg gcgggaaggt cgcagcgggg
    22081 gagagcgacg ctgccgcgtt ggcccgcgaa ctacacgagg aactcggcgt cgacgtgacc
    22141 gtgggggcac ggatcggcgc cgacgtcgcg ctcaacgaga tgaccacgtt gcgcgcgtac
    22201 cgcgtctcgc agaccggcgg ttcgttgcac cccaacgacc accgcgcgct gcggtgggtc
    22261 ggcgccgacg aactcgacac tctgccgtgg gtgcccgccg accgggcctg ggtcgccgag
    22321 ttgacccacc tgctgcggag cggcgccacc gtgcgcccgg cggtggaggc cgaccggaag
    22381 gccatcgcgg cggtcatcgt cgaggcctat cgagacgagt tctgcaccct cacgcgcaac
    22441 atggaaaaca tcagggccgc actcacgccc gcggtcgagg tcgatcgctt cttcgtcgcg
    22501 gaccgcgctg gcgacatcat cggcgccatc gcctgtaccg accacactgg ccgtgccatg
    22561 cgggttgacg cggccgactg gcgacgccgt ctcggtgtcg tacgaggcac ggtggccgcg
    22621 cgaatcctgg cttcgcagtg gatgtctgcg ctcgactacc cggcggcgac gggctacatc
    22681 gagttcgtcg ccgtggccga gcgggcccgg cgtcaaggcg tagccacgat gctcgtcgag
    22741 ggcgccattg cgcaaacgcc gtacaccgac tttgagctgg aggtcactga tgtcaacagc
    22801 cccgcccgcg actgctactg caagatcggg ttcgtcgacg gcagacggaa gaaagagaag
    22861 ttcgttcgat tcaagggatt ccgcgagcgg atctacatgc ggtactcgca ttgaccgtt
//
LOCUS       DASRJA010000424         3843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3415778,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000424 DASRJA010000000
VERSION     DASRJA010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3415778"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="VFC01_18485"
     CDS             <1..1297
                     /locus_tag="VFC01_18485"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN81650.1"
                     /translation="TLGYRFRPWTDEKAIADGPAILQYVHDTADEYCVKPHIRYGHKV
                     VSAEFSTADARWTVRAEHAGEEVELTCSVLLACSGYYNYDEGYTPEFAGSDDFEGTII
                     HPQHWPEELDYADKKIVVIGSGATAVTLIPALANSGAGHVTMLQRSPTYIGALPDVDP
                     FTVRTNKALPDKPAYVLNRWKSILFQSAQYNISRRFPNFMRKTLMTMAQRRLPEGYNV
                     EKHFGPSYNPWDQRLCLAPNGDLFKTIRAGKADVVTDTIERFTKTGIKLASGEELQAD
                     IIVTATGLNLQLFGGAEVMRNGIPIELNDTMAYKGMMLTHMPNMAFTIGYTNASWTLK
                     ADLVSEFVCRVLNYMDANGFDTVEPQHPGNSVDERPLMDFTPGYVLRALDYLPKSGHV
                     APWRLKQNYLLDLRLIRRGKVDDEALAFTKHRAVEAVSA"
     gene            complement(1303..1785)
                     /gene="rraA"
                     /locus_tag="VFC01_18490"
     CDS             complement(1303..1785)
                     /gene="rraA"
                     /locus_tag="VFC01_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HZN81651.1"
                     /translation="MTIEPRPTADLVDEIGPDVRSCDLQFTQYGGKSEFAGPLSTVRC
                     FQDNALLKSVLSEPGNGGVLVIDGDGSLHTALVGDLIAGLGVDNDWAGLIINGAVRDA
                     AALSTLPIGIKALGTNPRKSNKTGDGQRDVAVEFGGVVFAPGEIAYSDDDGIVVVAAD
                     "
     gene            complement(1782..2537)
                     /locus_tag="VFC01_18495"
     CDS             complement(1782..2537)
                     /locus_tag="VFC01_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZN81652.1"
                     /translation="MDANTVGAVAALTGVSVRTLHHYDHIGLVVPSVRTPAGYRGYTD
                     ADIERLHLVLVYRSVGMPLDEIRTLLDDPGADELEHLQRQHALLLEQADRLQHTIKAV
                     EELMNAHRSGIQLTAEEQIEIFGTTAFSEEYADEAEERWGETDAWKQSQQRVSQFSKE
                     DWIAIKAEGDALLEALAQAKRGGVQPGSAEANELAARHRASIERFYECGDDMHSGLVE
                     MYLADERFTRFYDDVEPGLAQFVHDIVVASINR"
     gene            complement(2580..3068)
                     /locus_tag="VFC01_18500"
     CDS             complement(2580..3068)
                     /locus_tag="VFC01_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81653.1"
                     /translation="MADQQDRPDEKPDAAAPAAEEAPSAPSPEPVPPAPEPPPAKEAP
                     AKAAKKAPAKAAKKAPAKAAKKAPAKKAAKKTAAKKAPPKKVPAAPKLAETNGDLTSA
                     AKEAAAQAKSTVASASNPVTGPAPVPLIGPEPSRLPLAAAIAAGVLAILVVLLARRHS
                     DD"
     gene            complement(3140..>3843)
                     /locus_tag="VFC01_18505"
     CDS             complement(3140..>3843)
                     /locus_tag="VFC01_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HZN81654.1"
                     /translation="IASAIWLVAELLRLGVAAAQAAAVPVTRLGVHTAVDFALHTTPG
                     RSGLFSAVAAGLVCLAVVAAPRSASTNIAVTGLTAAGMAARPLTGHFSESALGGVAVA
                     VHTLAAALWCGALASVVLTVEHRGQWARVLPRFSQLSLGCVTALLVGGLLGAAVTLGG
                     PSQLYSTGYGRLLSAKVAVTVLLVALAYRNRTMWLPAARSHRTTAVVSRSRALFELAI
                     MAVALTLAAALAVTG"
BASE COUNT          629 a         1333 c         1230 g          651 t
ORIGIN      
        1 gacgctcggc taccgcttcc gcccgtggac ggacgagaag gcgatcgccg acggcccggc
       61 gatcctccag tacgtccacg acacggccga cgagtattgc gtcaaaccgc acatccgcta
      121 cggccacaag gtggtcagcg ccgagttctc caccgccgac gcgcggtgga ccgtgcgcgc
      181 cgagcacgcc ggcgaggagg tcgagctgac ctgctccgtc ctgctggcct gcagcggcta
      241 ctacaactac gacgagggct acacacccga gttcgccggt tcggacgact tcgagggcac
      301 gatcattcac ccgcagcact ggccggagga gctggactac gcggacaaga agatcgtggt
      361 catcggcagc ggtgccaccg cggtgactct gattccggcc cttgccaatt cgggcgccgg
      421 gcacgtcacg atgctgcagc gctcgccgac ctacatcggt gcgctgcccg atgtggaccc
      481 gttcaccgtg cggacgaaca aggcgctgcc ggacaagccc gcctacgtgc tgaaccggtg
      541 gaagagcatc ctgttccagt cggcgcagta caacatcagc cggcgcttcc cgaacttcat
      601 gcgcaagacg cttatgacca tggcgcagcg gcggctgccc gagggctaca acgtcgagaa
      661 gcacttcggt cccagctaca acccgtggga tcagcgactc tgcctggcgc ccaacggcga
      721 tctgttcaag acgatccgcg cgggcaaggc cgacgtcgtc accgacacga tcgagcgctt
      781 caccaagact ggtatcaagc tggcctcggg cgaggagctt caggccgaca tcatcgtcac
      841 cgccaccggt ttgaacctgc agttgttcgg cggtgccgag gtgatgcgca acggcatacc
      901 gatcgagctc aacgacacca tggcctacaa gggcatgatg ttgacgcaca tgcccaacat
      961 ggctttcacc atcggctaca ccaatgcgtc gtggacactg aaggccgatc tggtctcgga
     1021 gttcgtctgc cgggtgctga actacatgga cgccaatggc tttgacaccg tcgagccgca
     1081 gcatcccggc aactcggtcg acgagcgccc gctgatggac ttcactcccg gctacgtgct
     1141 gcgtgcgctg gactacctgc ccaaatcggg acacgtcgcg ccgtggcggc tcaagcagaa
     1201 ctatctgctg gacctacgcc tgatccggcg cggcaaggtc gacgacgagg cgctggcgtt
     1261 caccaaacac cgggcagtag aggcggtttc ggcctagttg ggctagtctg cggcgacgac
     1321 gacaatgccg tcgtcgtcgc tgtaggcgat ctcgcctggc gcaaacacga ctccgccgaa
     1381 ctcgacagcg acatcgcgct gcccgtcgcc ggtcttgttg ctcttgcgcg gattggtgcc
     1441 gagcgccttg atgccgatcg ggagtgtgct cagtgccgcc gcgtcgcgga ccgcgccgtt
     1501 gatgatcagc ccggcccagt cgttgtccac gccgaggccg gcgatgaggt cgccgaccag
     1561 tgccgtgtgc agtgagccgt cgccgtcgat cacaagcact ccgccgttgc cgggctcgga
     1621 cagcaccgac ttcagcagtg cgttgtcctg aaagcaccgc accgtggaga gcgggcctgc
     1681 gaactcggac ttgccgccgt actgggtgaa ttgcagatcg caactacgaa cgtcggggcc
     1741 gatctcgtcg acaaggtcag cggtggggcg gggctcgatg gtcaccgatt gatgctagcc
     1801 accacgatgt cgtgcacgaa ctgggccagc ccgggctcaa cgtcgtcgta gaagcgcgtg
     1861 aaccgctcgt cggccagata catctccacc agcccgctgt gcatgtcgtc gccgcattcg
     1921 tagaaccgct cgatcgacgc gcggtgtcgc gccgccagct cgttcgcctc ggccgatccg
     1981 ggctgtaccc caccgcgctt ggcctgcgcg agcgcctcga gcagtgcgtc gccctcggcc
     2041 tttatcgcga tccagtcctc cttgctgaac tgcgaaaccc gttgctggga ctgcttccac
     2101 gcgtccgtct cgccccaacg ttcctcggcc tcgtcggcgt actcctcgct gaacgcggtc
     2161 gtgccgaaga tctcgatctg ttcctccgcg gtcagttgaa tgccgctgcg atgtgcgttc
     2221 atcaattcct ccactgcctt gatcgtgtgc tgcagccggt cggcctgctc cagcagtagc
     2281 gcgtgctgcc gctgcaggtg ctcgagttcg tccgcacccg ggtcatcgag cagcgtgcgg
     2341 atttcgtcga gcggcatccc gacggagcgg tacaccaaca ccaggtgcaa ccgctcgatg
     2401 tcggcgtcgg tgtagccgcg gtagcccgcc ggggtacgga cgctcggcac cacgagaccg
     2461 atgtggtcgt agtggtgcag ggtgcgcacg gagacacccg tgagggcggc taccgcgccg
     2521 acggtgttgg cgtccatgcc gccactatcg accctgacgc cgcgtgaggg tcaagccgat
     2581 tagtcgtcgg aatgtcgcct agccagcagc accaccagaa tcgccagcac accggccgcg
     2641 atggcggcgg ccagcggcag ccgtgacggc tcgggcccga tcaacggcac cggtgctggg
     2701 ccggtcaccg gatttgatgc gctcgcaacg gtggacttcg cttgtgccgc agcctctttc
     2761 gcggccgacg tcagatcgcc gttggtctcg gccagcttcg gagcagcagg taccttcttc
     2821 ggtggcgcct tcttcgcagc ggttttcttg gcggccttct tcgccggtgc tttcttggcg
     2881 gccttggcgg gagccttctt ggccgccttg gcaggagcct tcttcgcggc cttggccggc
     2941 gcctccttcg caggaggcgg ttccggggcg ggcgggaccg gttctgggct cggcgccgac
     3001 ggtgcctctt cagctgccgg tgctgcggcg tcaggctttt cgtcgggtcg atcctgctgg
     3061 tctgccatct ggctgctcct ttgcgcctgt tttcgtctac ccggcagtgg ctcccatcat
     3121 gccagggtcg gatgcggcat taaccggtca ccgccagcgc ggccgccagt gttagcgcga
     3181 ctgccatgat cgccagttcg aataacgccc gcgaccgcga caccacggcc gtggtgcgat
     3241 gcgaacgcgc ggcgggcagc cacatcgtcc gattgcggta cgccaaggcg acgagcagca
     3301 cggtcaccgc gaccttcgcg gacaacagcc gaccgtaccc cgtcgaatac agctgactcg
     3361 gcccgccgag ggtcacggcc gcccctagca acccgcctac cagcagcgcc gtcacgcatc
     3421 ccagcgacag ttgcgagaac ctcggcagca ccctcgccca ctggccgcgg tgctcgaccg
     3481 tgagcaccac cgaagccagc gcgccgcacc atagcgccgc cgcgagagtg tgcacggcca
     3541 cagccacccc tcccagcgcg ctctcggaga aatggccggt gagaggccgc gcggccattc
     3601 cggcagcggt gagacccgtt actgcgatat tcgtcgacgc cgaacgcggt gccgccacca
     3661 ccgccaggca caccaggccc gctgccaccg cgctgaacag ccccgaccga ccgggcgtcg
     3721 tgtgcagggc gaaatcgacc gcggtgtgca cgccgaggcg cgtgacgggc accgccgcgg
     3781 cctgcgcggc cgccacaccg agccgaagta gctcggcgac gagccagatg gcgctggcga
     3841 tcg
//
LOCUS       DASRJA010000425        10190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_832063,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000425 DASRJA010000000
VERSION     DASRJA010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10190
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832063"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFC01_18510"
     CDS             complement(<1..509)
                     /locus_tag="VFC01_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HZN81655.1"
                     /translation="MCGRFAVTTDPALLAEKIQAIDEATSAQKDTTGPNYNVAPTTTI
                     STVVKRHTEPDDESTRRVRLMRWGLVPPWAKTADEGGPDTKGPLLINARSEKVTSSPA
                     FRNSAKSKRCLVPMDGWYEWRPNGELASGKKAPKTPFYMYGGDGEMLFMAGLWSTWRP
                     KNAPKDAPPL"
     gene            554..1834
                     /gene="aroA"
                     /locus_tag="VFC01_18515"
     CDS             554..1834
                     /gene="aroA"
                     /locus_tag="VFC01_18515"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZN81656.1"
                     /translation="MSNWQAPSTPTPVHATVTIPGSKSLTNRALVLAALATSNGTSTI
                     SGALRSRDTDLMIGALQTLGVAVEGAGSELTIGGRIAPQPDARVDCGLAGTVLRFVPP
                     VAALSTERVLFDGDEQARARPIAPLLDALRSLGVDIDGDGLPFAVRGTGSVAGGTVEI
                     DASASSQFVSGLLLSGAAFADGLTIVHTGESVPSAPHIAMTVSMLRDAGVELDDTQAN
                     RWRVAPGPIAARHWVVEPDLSNAVPFLAAAVVSGGIVRVTGWPEGSIQPADDVFAILE
                     KLGSVVRHGSSYLEVQGAQRYGGFDVDLHDVGELTPAVAALAALASDGSVSQLSGIAH
                     LRGHETDRLAALSAEINRLGGQCEETPDGLVVTAGPLHGGVWRSYADHRMATAGAIVG
                     LRVPGVEVEDIGTTAKTLPDFPQLWADMLQGQAD"
     gene            1831..2811
                     /gene="rsgA"
                     /locus_tag="VFC01_18520"
     CDS             1831..2811
                     /gene="rsgA"
                     /locus_tag="VFC01_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZN81657.1"
                     /translation="MSPREYDESDVRVRPGRGSRPRTKTRPDHADAQEAMVVTVDRGR
                     WGCALGRDPDHLVTAMRARELGRTSIVVGDDVGIVGDLSGKTDTLARIVRRGERRTVL
                     RRTADDTDPTERVVVANADQLLIVVALADPPPRTGLVERTLIAAYAGGLEPILCLTKT
                     DLAPPEPFAEQFADLDLTITTAGRDDPLDVVAPLLADKVTVLLGHSGVGKSTLVNRLV
                     PEADRATGDVSGVGKGRHTSSQSVALPLSFGGWVIDTPGIRSFGLAHIEPDDVMLAFS
                     DLAEAIKDCPRGCGHMGPPADPECALDTLAGAAASRVGATRRLLAALRES"
     gene            2935..3588
                     /locus_tag="VFC01_18525"
     CDS             2935..3588
                     /locus_tag="VFC01_18525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN81658.1"
                     /translation="MTAPDFLQSTRDGYDLTAAMYAERFHGHLDDKPLDRAMLAGFAG
                     LVERNGVIADVGCGTGATSRILADFGLDVLGIDLSPNMIAEAHRLNPDLRFQVGSMTS
                     INADDNHFHGICAWYSVIHIPDELLPQVFSEFRRVLRPDGWALVAFQVGEQPRTFKEL
                     FGEQVALTFFRRQPDVVAQLLEEAGLEPYAGLVREPNDDGFESTPHAYLIARNCALD"
     gene            complement(3662..4918)
                     /locus_tag="VFC01_18530"
     CDS             complement(3662..4918)
                     /locus_tag="VFC01_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZN81659.1"
                     /translation="MAITDVPAFAHLTEADIENLAVELDAIRLDIEDSRGERDSRYIR
                     RTIAAQRALEVTGRLMLAASSRRSAWWAGTVTLGVAKIIENMEIGHNVMHGQWDWMND
                     PEIHSSTWEWDMSGASKHWRFTHNFMHHKYTNILGMDDDVGYGLLRVTRDEKWKPFNL
                     GNLLYNTMLALGFEWGVGLQHLELGRMFKGRDDRDATMVRLREFSAKAGRQVVKDYVA
                     YPAMTSLSPGATFKSTLKANAVANVIRNVWSNAVIFCGHFPDGAEKFTKTDMVGETKG
                     EWYLRQMLGSANFEGGPALRFMSGNLCHQIEHHLYPDLPSNRLHEISIRVREVCDKYD
                     LPYTTGSFLLQYGKTWRTIAKLSLPDQYLRDTADDAPETRSERMFTELEPSQRRGLKS
                     AIAAVRARRREKRAEKRTPKRDDLAA"
     gene            complement(4988..6229)
                     /locus_tag="VFC01_18535"
     CDS             complement(4988..6229)
                     /locus_tag="VFC01_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HZN81660.1"
                     /translation="MVLHWLKRRTYGTVGYVSRATGSAILKVGQELSLGKKYSSSRPA
                     AVLKDTVRPTVAGAGKHRGWDTARAIAERITTPLLPDDYLKLANPLWSARELRGRVLE
                     VRRETVDSATLVIKPGWGFSFDYQPGQYIGIGLLVDGRWRWRSYSLTSSPVTGSRTIT
                     ITVKAMPEGFLSTHLVGGVAPGTIVRLAAPQGNFVMPDPAPASVLFITAGSGITPVMS
                     MLRTLARRDQITDVVHLHSAPTESDVMFAAEMAKMQTGHEGYRLRLRSTRTEGRLDLS
                     QLSDEVPDWRERQTWACGPEGMLNDAERVWAAAGIAGQLHLERFAVSKAAAHGQGGTV
                     QFARSQKTVSVDGATPLMDAGEEVGIQMPFGCRMGICQSCVVGLLDGHVRDLRTGAEH
                     EPGSRVQTCISAASGDCVLDV"
     gene            complement(6229..6729)
                     /locus_tag="VFC01_18540"
     CDS             complement(6229..6729)
                     /locus_tag="VFC01_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81661.1"
                     /translation="MVRVYVPATLAMLQQLVADRLLHARNGTAFAVTPTLREAYAEGD
                     DDELADVALREAALASLRLLAGEGTSDLPPRRAVVVAKVEGATARPDLDDAVVRLSEP
                     IGYDDVIAAYVDNADAEPAVLPAIEAIDAADLGDEDAEFVVGDAQDYDLAWYAPQELP
                     FLLELL"
     gene            complement(6743..8152)
                     /locus_tag="VFC01_18545"
     CDS             complement(6743..8152)
                     /locus_tag="VFC01_18545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZN81662.1"
                     /translation="MVTRLSASDASFYHLEDSSTPMYVGTLSILRKPRNGLSYETLLA
                     TVEQRLPQIPRYRQKVREVTLGLARPVWIDDRDFDITYHIRRSALPSPGSDAQLHELI
                     ARLGSRPLDKSRPLWEMYLIEGLARNRIAIYTKSHQALVNGMTALEIGHVIADRTQKP
                     PEFGEDIWIPAREPSDRALVLGAVGEWITRPAEQFGAVRSALTEVATNAGQLVEVGRR
                     VVDVARTFARGTAPNSPLNTTVSRNRRLAVASHQLDDYRVVRARYECDVNDVVLAVVA
                     GALRNWLMSRGEPVTSTTAVRAMAPMSVYPDADLDTTGPGQAISEVSPFLVDLPVGEG
                     NAVVRLSQIAHATESVSTAGSLVDARTIVTLSGFAPPTLHAMGIRVATSFSARQFNLL
                     ITNVPGAQKQMYVAGTKLLETYAVPPLLNNQVLALGVTSYNGMLYFGINADRDAMSDV
                     DVFPSLLRESLDELLEAAQ"
     gene            8334..8813
                     /locus_tag="VFC01_18550"
     CDS             8334..8813
                     /locus_tag="VFC01_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HZN81663.1"
                     /translation="MTASGIPSEEVSLTTPIIDCEPPPVGITACPPPSPAALHRRTTR
                     HLRPVPRPPRDPLPPRAAMLFADAALRRVLEVVDRRRPIAQLRPLVTPALIDTVTALT
                     RAPHTAAATLRRVRVRMVDSESAEVFGTYTRGPRTLAVAARIELHRDRWRIAAFQLD"
     gene            8832..9299
                     /locus_tag="VFC01_18555"
     CDS             8832..9299
                     /locus_tag="VFC01_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN81664.1"
                     /translation="MQGNTVSVERVIKAPAAQIFALIADAGKHSSFDGSGTVDHSPQG
                     SQPLQLGSTFGMSMRGRPESLFLPYRTTNTIIEFEPDRRIAWKTTIGPLGLIGGRIWR
                     YELSPAEGGTLVRETWDVSQDRQRPMLKTGAMPKQAEDGMRATLERIAAIVEP"
     gene            complement(9301..>10190)
                     /locus_tag="VFC01_18560"
     CDS             complement(9301..>10190)
                     /locus_tag="VFC01_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZN81665.1"
                     /translation="KYDEVMNQQRKVIYEERRRILEGENLAEQAHQMLVDVVTAYVDG
                     ATAEGYSEDWDLEQLWTALKQLYPVGIDHHDLIDSDAVGEAGELTRDELLDALVEDAK
                     RAYADREKQLEEIAGDGAMRQLERNVLLNVLDRKWREHLYEMDYLKEGIGLRAMAQRD
                     PLVEYQREGFDMFVGMLEGLKEESVGFLFNVQVEAAPAPTVAPVAAPAGLAQFAAEAA
                     AKAQDSAVATKERPAGLRAKGIEDSAPPLTYTGPSEDGSVQRSGGGGKHAASTGTSRR
                     ERREAARQQAKSAKGSRRR"
BASE COUNT         1609 a         3473 c         3329 g         1779 t
ORIGIN      
        1 agcggcggcg cgtccttggg tgcattcttc ggccgccacg tcgaccacag gcccgccatg
       61 aacagcatct cgccgtcgcc gccgtacatg tagaacgggg tcttcggcgc cttcttgccc
      121 gacgccagtt caccgttggg ccgccactcg taccaaccgt ccatcggtac caggcaccgc
      181 ttgctcttcg cggagttgcg gaacgcaggt gagctggtca ccttctcgct gcgcgcgttg
      241 atcaacaacg gccccttggt gtcgggtccg ccttcgtcgg cggttttcgc ccacggcggc
      301 accaggcccc accgcatcaa ccgcacgcgg cgcgtcgact cgtcatccgg ttcggtgtgt
      361 cgtttcacga ccgtactgat cgtggtggtc ggcgcgacgt tgtagttggg cccggtcgtg
      421 tctttctgcg ccgacgttgc ctcgtcgatg gcctggatct tctccgccag cagggccgga
      481 tcggtggtca ctgcgaatcg cccacacata tatcccatgg tggcacgcac aaccgacagt
      541 gcaggataga gccgtgagca attggcaggc accgtcgacg ccgacccctg tgcatgccac
      601 cgtcacgatc cccggctcca agtcactgac caaccgggcg ctcgtgctgg ccgcgctggc
      661 aacgtcgaac gggacctcga ccatcagcgg tgcgctgcgc agccgcgaca ccgacctgat
      721 gatcggggcg ctgcagaccc tcggcgtcgc cgtcgagggc gcaggctccg agctgacgat
      781 cggcggtcgc atcgccccgc agccggatgc ccgtgtcgac tgcggcctgg ccggcacggt
      841 cctgcgcttc gtcccaccgg tggcagcgct gagcaccgag agggtgctct tcgacggcga
      901 cgagcaggcc cgcgcccggc cgatcgcgcc gctgctcgat gcgcttcgca gcctcggggt
      961 ggacatcgac ggtgacggtc tgccgttcgc cgtgcgcggc accgggtcag tggccggcgg
     1021 cacggtggag atcgacgcgt cggcgtcgtc gcagttcgtc tcgggtctgt tgctgtccgg
     1081 tgcggcgttc gccgatgggt tgacgatcgt gcacaccggc gagtcggtgc cgtcggcgcc
     1141 gcacatcgcg atgacggtgt cgatgctgcg cgacgccggc gtcgagctcg acgacacgca
     1201 ggccaaccgg tggcgtgtgg cgcccggccc aattgcggcg cgacactggg ttgttgagcc
     1261 cgacctgtcc aatgccgtgc cgttcctggc cgcggccgtg gtcagcggtg gcatcgtccg
     1321 ggtgaccggc tggccggagg gcagcatcca gcccgccgac gatgtgttcg cgatcctcga
     1381 gaagctgggt tcagttgtgc gccacggtag ttcgtatctg gaggtgcagg gcgcacagcg
     1441 ctacggcggc ttcgacgtcg acctgcacga tgtcggggag ctgacgcccg cggtggccgc
     1501 tctggcggcg ttggcgtcag acggatcggt gtcgcagctc agcgggatcg cgcatctgcg
     1561 cggtcacgag accgatcggc tcgcggcgtt gtcggccgag atcaaccggc tcggtgggca
     1621 atgcgaggag acacccgacg gactggtcgt caccgcgggg ccgttgcacg gcggcgtgtg
     1681 gcggtcgtac gccgaccatc ggatggcgac ggcgggtgcg atcgtcgggc tgcgggtgcc
     1741 cggtgtcgag gtggaagaca tcggcacgac ggcgaagacg ctgccggact tcccgcagct
     1801 gtgggccgac atgctccaag ggcaggccga ttgagccctc gcgagtacga cgagtccgat
     1861 gtccgggtcc ggcccggccg gggctcgcgg ccgcgaacca agactcgccc cgaccacgcc
     1921 gacgcgcagg aagccatggt ggtcaccgtg gatcgcggcc ggtggggatg cgcgttgggt
     1981 cgtgacccgg atcatctcgt gacggccatg cgggccaggg agttgggccg cacctcgatc
     2041 gtggtcggcg acgacgtcgg catcgtcgga gatctgtccg gcaagacgga caccctggcc
     2101 cgcatcgtgc gccgtggcga gcggcgaacg gtgttgcggc gcacggccga tgacaccgat
     2161 cccaccgaac gcgtggtcgt cgccaacgcc gaccaactcc tcatcgtggt cgctctggct
     2221 gacccgccac cgcgcaccgg cttggtcgag cgcactctga ttgccgccta cgcaggcggg
     2281 ctcgagccga tcctgtgtct gaccaagacc gatctcgccc ctccggagcc gttcgccgaa
     2341 caattcgccg atctcgacct cacgatcacc accgcgggcc gcgacgatcc actcgacgtc
     2401 gtcgccccgc tgctggccga caaggtgacg gtgctcctcg gccattccgg tgtcggcaaa
     2461 tccacgctgg tgaatcgcct tgtaccagag gcagatcggg ccacaggcga tgtgtcggga
     2521 gttggcaaag gccgacatac gtcgtcccag tcggtggcgc tgccgctgag cttcggcggc
     2581 tgggtgatcg acacgccggg tatccggtcg ttcgggctgg cgcacatcga acccgacgac
     2641 gtgatgctgg cgttctcgga tttggccgag gcgatcaagg actgcccgcg cgggtgcggg
     2701 cacatgggtc cacccgccga cccggagtgc gcgctggaca cgttggcggg ggccgcagcg
     2761 agccgggtcg gcgcgacgcg ccgcctgttg gccgcactgc gggagagcta gcccaccttt
     2821 tccgcgagcg tgcgtgtctg tacgccgaca cgccgcaatt tccgagcatt ttgtgcacgt
     2881 ccccagcttg cgcgtgggcc cacgcgcccg gtgtggaccc gtagagtgcg acgggtgacc
     2941 gcaccggact ttctgcaaag tacccgcgat ggttacgatc tcacggccgc gatgtacgcc
     3001 gaacgctttc acggtcacct cgacgacaag cccctcgatc gcgcgatgct ggccggattc
     3061 gccggattag tggaacgcaa cggtgtcatc gccgacgtcg gctgcggcac aggcgctaca
     3121 tcgcgaatcc tcgcagactt cgggttggac gttctcggta tcgacttgtc ccccaacatg
     3181 attgccgaag cacaccgact caacccggac ctgcgcttcc aagtcggatc aatgaccagc
     3241 atcaacgcgg acgacaacca ctttcatggg atctgcgcgt ggtactcggt gatccacatt
     3301 cccgacgagc tactgcctca ggtcttttcg gaattccgtc gcgtgctgcg tcccgacggc
     3361 tgggcgttag tggcattcca agtcggtgaa caaccccgca ctttcaagga gttgttcggc
     3421 gagcaggtgg ccttgacgtt ctttcgccgc cagccggacg ttgtggcgca gttgttggag
     3481 gaggcgggcc tagagccgta cgcagggttg gtgcgtgaac ctaacgacga cggcttcgaa
     3541 tccacaccgc atgcctattt gatcgctcgc aactgcgcgc tcgattagcc gcgagcgtgc
     3601 ggaaaatgca cgcgaaatac ggcgtgtcgg cgcacagaca cgcacgctcg cggattaggc
     3661 gtcaggcggc caggtcgtcg cgcttgggcg tccgcttctc agcccgcttc tcgcgacggc
     3721 gtgcccgcac tgcggcgatg gccgacttga gcccacggcg ctgactcggc tccaactcgg
     3781 tgaacatccg ctcgctgcgg gtctccgggg catcgtcggc ggtgtcgcgc agatactggt
     3841 ccggtagcga cagcttggcg atcgtccgcc acgtcttgcc gtactggagc aggaacgacc
     3901 ccgtggtgta cggcaggtcg tacttgtcgc acacctcgcg cacccgaatc gatatctcgt
     3961 ggagccggtt actcggcaga tccggataca ggtggtgctc gatctggtgg cacaggttgc
     4021 cgctcatgaa acgcagcgca ggcccgccct cgaagttggc gctgcccagc atctggcgca
     4081 ggtaccactc ccccttggtc tcaccgacca tgtcggtttt ggtgaatttc tctgcgccat
     4141 cagggaagtg gccgcagaag atcaccgcgt tggaccacac gttgcgaata acgttcgcta
     4201 ccgcgttggc cttcaacgtc gacttgaacg tcgcgccagg cgacagagag gtcattgcgg
     4261 ggtaggcgac gtagtccttc acgacctggc ggccggcctt cgccgagaac tcgcgcagcc
     4321 gcaccatggt ggcgtcgcgg tcatcgcggc ccttgaacat cctgccgagt tccagatgct
     4381 gcagccccac gccccactcg aatccgagcg cgagcatggt gttgtacagc aggttgccga
     4441 ggttgaacgg cttccacttc tcgtcgcggg tgacccgcag caggccatag cccacgtcgt
     4501 cgtccatgcc gaggatgttg gtgtacttgt ggtgcatgaa gttatgggtg aaccgccagt
     4561 gcttggacgc cccactcatg tcccactccc acgtcgagga gtgaatctcg ggatcgttca
     4621 tccaatccca ctgcccatgc atgacgttgt ggccgatctc catgttctcg atgatcttgg
     4681 ccacgcccag agtcacggtg cctgcccacc acgccgagcg ccgcgagctc gcggccagca
     4741 tcagccggcc ggtcacctcg agggcacgct gggcggcgat ggtgcggcgg atgtagcgcg
     4801 agtcgcgctc accacgggag tcttcaatgt ccaggcggat cgcgtcgagt tcgacggcca
     4861 ggttttcgat gtcggcttcg gtcagatgcg cgaatgccgg tacgtcggta attgccattg
     4921 ttctgcctcc cttctcttac ctacgctacc gtaacctacg aatccgtagg ttaccagtca
     4981 gtaaagctta aacgtccagt acacaatcgc cggatgcggc ggagatgcag gtctgcaccc
     5041 ggcttccggg ctcgtgttcc gcgccggtgc gaaggtcgcg gacgtgtccg tcgagcagcc
     5101 cgacgacgca cgactggcag atgcccatcc ggcagccgaa tggcatctgg atgccgactt
     5161 cctcgccggc gtccatcaac ggcgttgccc cgtccacgct gaccgtcttc tgactgcgcg
     5221 cgaactgaac cgtgccgccc tgaccatgtg cagcggcctt cgacaccgcg aagcgctcca
     5281 gatggagctg cccggcgata cccgcggccg cccacacccg ctcggcgtcg ttgagcatgc
     5341 cctccggccc gcaggcccag gtctggcgct cacgccagtc gggcacctca tcgctcagct
     5401 gggacaggtc gaggcggccc tcggtacggg tggaccgcag ccgcagccga tatccctcgt
     5461 gaccggtctg catcttcgcc atttcagcgg cgaacatcac atcggattcc gttggcgccg
     5521 aatgcaagtg gaccacgtcc gtgatctggt cgcggcgggc cagcgtgcgc agcatcgaca
     5581 tcaccggtgt gatgccggag cccgccgtga tgaacaacac cgaagccggc gctggatcgg
     5641 gcatcacgaa gttgccctgc ggcgcggcca gccgcacgat cgtgccgggg gctaccccgc
     5701 cgaccaggtg ggtcgacagg aatccctcgg gcatcgcctt gaccgtgatg gtgatggtgc
     5761 ggcttcccgt caccgggctc gacgtcagcg aatacgaccg ccaccgccag cgtccgtcga
     5821 ccaacaggcc gatcccgatg tactggccgg gctgatagtc gaaggaaaag ccccatcccg
     5881 gcttgatgac caaggtcgcg gagtcgacgg tttcgcggcg cacttccagc acccgaccgc
     5941 gtagttctcg cgccgaccac aacgggttcg cgagcttgag gtagtcgtcg ggtaacaacg
     6001 gcgtggtgat ccgctcggcg atcgctcgtg ccgtatccca gccgcgatgt ttacctgcac
     6061 cggcgacggt cggccgcacg gtgtccttga ggacggccgc gggcctgctg ctgctgtatt
     6121 tcttgcccaa ggaaagctcc tgcccaactt tcaggattgc gcttcccgta gctcgggaga
     6181 cataacctac ggtaccgtag gttcggcgct tgagccaatg gagaaccatc acagcagctc
     6241 gagcaggaac ggcagctctt gcggcgcgta ccaggcaagg tcgtagtcct gcgcatcgcc
     6301 gacgacgaac tcggcgtcct catcccccag gtcagccgcg tcgatggcct cgatcgccgg
     6361 cagcaccgcc ggctcggcat cggcgttgtc aacatacgcg gcaatcacgt cgtcgtatcc
     6421 gatcggctcc gacagtcgca ccaccgcgtc gtcaagatcc ggacgcgccg tggcaccctc
     6481 cactttggcg accaccacgg ctcgccgcgg cggcagatcg gatgtcccct cgccggccaa
     6541 cagccgcagc gaggccagcg ccgcttcgcg caacgccacg tcggcgagtt cgtcgtcgtc
     6601 gccctcggcg taggcctcgc gcagcgtcgg ggtgaccgcg aacgcggtgc cgttgcgcgc
     6661 gtgcagcagt cgatcggcga ccagctgctg cagcatcgca agggtcgcgg ggacatagac
     6721 acgcaccagg cgaggttagc ggttactgcg cggcttccag cagttcgtcg agcgattccc
     6781 gcagcagaga cggaaataca tcgacgtcgc tcatcgcatc gcggtcggcg ttgatgccga
     6841 agtagagcat gccgttgtag gacgtcaccc cgagcgccag cacctggttg ttcagcagcg
     6901 gtggcaccgc ataggtttcc agcagcttgg tgcccgcgac gtacatctgc ttctgcgcgc
     6961 ccggcacatt ggtgatcagc agattgaact gccgcgccga gaaactggtc gcaacgcgaa
     7021 ttcccatggc gtgcaacgtc ggtggcgcaa agcccgatag cgtgacgatg gtcctggcat
     7081 caaccaaact gcccgccgtc gacaccgact cggtggcatg tgcgatctgc gacagccgca
     7141 ccaccgcgtt gccctcaccg acgggcaggt cgacgagaaa tggcgacacc tcgctgatcg
     7201 cctgtccggg tccggtcgtg tcgagatcgg cgtcgggata caccgacata ggtgccatcg
     7261 cgcgaacggc cgtcgtcgag gtgaccggct ccccgcgcga catcagccag ttccgcagcg
     7321 cgccggccac cactgcgagc acgacgtcgt tgacatcgca ttcgtagcgc gcccgcacca
     7381 cgcgatagtc gtccaactga tggctggcga cggcaaggcg gcggttgcgc gagaccgtgg
     7441 tgttcagcgg gctgttcggc gccgtgcccc gggcgaaggt gcgtgccacg tcgacgacgc
     7501 ggcgcccgac ctcgaccagc tgaccggcgt tggtcgccac ctcggtcagc gccgagcgga
     7561 ccgccccgaa ctgctcggcc ggacgggtga tccactcacc gaccgctccg aggaccagcg
     7621 cacggtcgct cggttcgcgc gccgggatcc agatgtcctc accgaactcc ggcggctttt
     7681 gggtgcgatc ggcgatgacg tggccgatct ccagcgcggt catgccgttc accaacgcct
     7741 ggtgcgattt ggtgtagatc gcaatgcggt ttcgggccag accctcgatc agatacatct
     7801 cccacagcgg ccgggacttg tccagcggcc gcgagccgag ccgggcgatg agctcgtgca
     7861 gctgcgcgtc gctgcccggg gacggcagcg ccgatcgcct gatgtggtag gtgatgtcga
     7921 agtcgcggtc gtcgatccac acggggcggg ccaggcccag cgtcacctca cgcaccttct
     7981 ggcggtagcg cgggatctgc gggagccgct gttcgacggt ggccagcagc gtctcatagc
     8041 tcagcccgtt gcgtggcttg cgcaggatcg acagcgtccc gacatacatc ggggtggagc
     8101 tgtcttccag gtgatagaag gaggcatccg acgccgacaa cctcgtcacc attgcggcgt
     8161 tcgccctccc cttcatgttt gtcgctagca ctgaaccccc cgagaggtta tcggccggtt
     8221 ctgtgtgggc gcatcacggg tgcattcggt gtcgtcgaac cgtgcgatca tgaatgtgtc
     8281 tgcgactctc cagcagatgc cgtctctgtc gcaccgaacg ctggagagta ccgatgaccg
     8341 catccgggat tccgtccgaa gaggtgtcgc tcacgacgcc gatcatcgat tgtgagccgc
     8401 cgcccgtcgg aatcaccgca tgcccgccac cgtcgccggc cgcgctacat cgccgtacca
     8461 cgcgccattt gcggccagtg ccgcggccac cccgcgaccc gctgccgccc cgcgcagcga
     8521 tgctgttcgc cgacgcggcg ctgcgccggg ttctggaggt cgtggaccgc cgcaggccta
     8581 tcgcgcagct ccgtccgctt gtgacgcccg cgctcatcga taccgtcacg gcgctgacgc
     8641 gagcgccgca caccgccgcc gcgaccctgc gccgggttcg ggtgcgcatg gtcgacagcg
     8701 agtcggccga ggtgttcggc acatataccc gcgggccgcg cacgctcgcg gtcgcagcgc
     8761 ggatcgaact gcaccgtgac cgctggcgca ttgccgcctt tcagctcgac tgaggcggct
     8821 agcgttacgg cgtgcaagga aacaccgtca gcgtcgagcg ggtcatcaag gcgcccgccg
     8881 cccagatctt cgcgctaata gccgatgccg gaaagcattc cagcttcgac ggatccggga
     8941 ccgtcgacca ctcgccgcaa ggatcccagc cgctgcaact cggctcgacg ttcggcatgt
     9001 cgatgcgcgg acggccggaa tcccttttcc tgccctaccg caccaccaac accatcatcg
     9061 aattcgagcc cgaccgccgc atcgcatgga agaccacgat cgggccgctc gggctgatcg
     9121 gcgggcggat ctggcgctac gaactcagcc ccgccgaggg cggcacgctg gtgcgtgaga
     9181 catgggacgt ctcgcaggac cggcaacgcc cgatgctgaa gacgggcgcg atgcccaagc
     9241 aggccgagga cggcatgcgc gccacactgg agcgcatcgc cgcgatcgtc gagccgtgag
     9301 ttagcgcctg cggctgccct tggccgactt ggcctgttgg cgcgcggctt cacggcgctc
     9361 acgccggctg gtgccggtcg aggcggcatg cttaccacca ccgccggacc gctgcaccga
     9421 cccatcctcg gacggcccgg tgtacgtcag cggcggggcg gagtcctcaa ttcccttggc
     9481 gcgcaagccc gccgggcgct ccttggtggc gacggcactg tcttgcgcct tggccgcggc
     9541 ttcggccgcg aactgcgcca agcccgcggg cgcggcgacc ggcgccacgg ttggtgcagg
     9601 cgcggcttcc acctgaacgt tgaacaggaa gccgaccgac tcttccttga gcccctcgag
     9661 catcccgaca aacatgtcga agccctcacg ctggtactcg acgagcgggt cgcgctgcgc
     9721 catcgcccgc aggccgatgc cctccttgag gtaatccatc tcgtagaggt gctcacgcca
     9781 cttgcggtca aggacgttga gcagcacgtt gcgttccagc tgccgcatcg caccatcgcc
     9841 ggcgatctcc tcgagctgct tctcgcggtc ggcgtacgcg cgcttggcat cctcgaccaa
     9901 cgcgtcgagc agctcgtcgc gcgtcagctc gcccgcctca ccgaccgcgt cggagtcgat
     9961 caagtcgtgg tggtcgatgc cgaccgggta gagctgtttg agtgccgtcc acagctgctc
    10021 gagatcccag tcttcggagt agccctcggc cgtcgcgccg tcgacgtagg cggtgacgac
    10081 gtcgaccagc atctggtggg cctgctcggc gaggttctct ccctcgagga tgcggcggcg
    10141 ctcctcgtag atgaccttgc gctgctggtt catcacctcg tcgtacttga
//
LOCUS       DASRJA010000426         3979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3238079,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000426 DASRJA010000000
VERSION     DASRJA010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3238079"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VFC01_18565"
     CDS             <1..1081
                     /locus_tag="VFC01_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZN81666.1"
                     /translation="DLLAQARCSGKVPMVGGLLGPSAGHGALVAPVCDFTVMSGQGAI
                     FTAGPPVVKESTGEEISKEDLGGPSVALASGVIHNLAEDDEGVLDDIRRYLSYFPPSA
                     WSYPSTLPEDEAAEPRSTPELLDIVPRGNRRVYDMRTVLDVVFDARGWFEVQPKFGPA
                     IICALAHLGGNPVAVVANQPQVLAGSIDADAADKAAHFITVADSFHLPIVFLADNPGM
                     LPGSRSEKSGVLRSGARMFAAQTAATTLKLHVTLRKAYGFGSMVMSLLGFDNQSATFA
                     FPGATMGAMSAAALSKASHAADDVEAELRKMEVEASFRSASHLGFDDLIHPEETRNAL
                     LSALQRGLYSRQAAAEPVSRTVITP"
     gene            complement(1159..2280)
                     /locus_tag="VFC01_18570"
     CDS             complement(1159..2280)
                     /locus_tag="VFC01_18570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN81667.1"
                     /translation="MDSILAGRPTVDTYSRASHVAAVANRVDSYWVPDHINGQTPRPL
                     WDKKYTTAARLLRSPDAAMEAWTLLGSLAARRRFNRVRLGVGVTDTGKRHPAVTAQAA
                     ATLHLMTRGRAILGIGPGERAGNEPYGVDWSKPVARFEEALATIRALWDSKGELVNRD
                     SPFYPLRDAMFDLPPYKGKWPEIWIAAHGPRMLRATGRYGDAWFPGFAHRPEDYAKRL
                     EIIRSAASDAGRDPMAITPAFWMPVVAASNRDDVDAALETTAVKTWALNGPAEFYTHH
                     GAAHPMGADFAGMQDHVAFTMDERTIREKSSQVPLSVVNGMILNGTVDDVLEQAAVWR
                     DHGVRYIVVVNFGPMQPNIRGALATMLPFNKVVRRLKRL"
     gene            complement(2304..3347)
                     /locus_tag="VFC01_18575"
     CDS             complement(2304..3347)
                     /locus_tag="VFC01_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03857 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HZN81668.1"
                     /translation="MTERVLDELGYYLLAGAGGEGPATLMDEARRGEELGFGTAFISE
                     RWNVKEASSLVGAACAVTNRMQIATAATNHNTRHPLITGSWATTMHRLSGGRFTLGIG
                     RGVAAMYGAFGIPAVTTAQMEDFAQVMRRLWNGEVIFNHDGPIGKYQVLFLDPDFKED
                     IRLAIVAFGPRTLALGGRVFDDVILHTYFTPETLQRCVKTVKDAAEQAGRDPDSVRVW
                     SCFATVGDHLPEELRLKKTVARLATYLQGYGDLLVNTNGWDPAVLQRFREDSVVTSIP
                     GGIDHKATPEQIEHIATLIPDEWLEPSATGSAQQCVDRIRKEFDYGADALIMHGATPD
                     ELEPIVDAYRASP"
     gene            complement(3344..3718)
                     /locus_tag="VFC01_18580"
     CDS             complement(3344..3718)
                     /locus_tag="VFC01_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN81669.1"
                     /translation="MTRTAREVVEQYNLVVWNERDFALAEELIGDSVIRHEVGDTLTL
                     THEQAVQRIVDMWEVSEELRFDLNLVVAGDDGEHVAIVYESPIKLKDGTDTTVGSMEI
                     FRVIDGRITEVWNCGYKQGVWT"
     gene            complement(3715..>3979)
                     /locus_tag="VFC01_18585"
     CDS             complement(3715..>3979)
                     /locus_tag="VFC01_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HZN81670.1"
                     /translation="PTEARRGHDQQLVREYLQQLSEHGISDYSFDDAWRHYRFAVAYL
                     MLLPVIILIGWDALPERSRALCLKLTERAVATIEDIDALEVFG"
BASE COUNT          699 a         1293 c         1259 g          728 t
ORIGIN      
        1 tgatctgctg gcccaggcgc ggtgctccgg gaaggttccg atggtcggcg gattactggg
       61 tccgtcggcc ggacacggtg ccctggtggc accggtatgt gacttcaccg tcatgagcgg
      121 tcagggcgcg atcttcacgg cgggcccgcc ggtggtgaaa gagtcgacgg gcgaggagat
      181 ttcgaaggaa gacctcggcg ggccgagcgt cgcactggcc agcggggtga tccacaatct
      241 ggccgaggac gacgaaggtg tgctcgacga catccgccgg tacctgtcgt actttccgcc
      301 gagcgcatgg tcgtatccct cgacgctgcc cgaagacgaa gcggccgaac cgcgctcgac
      361 gcccgaactg ctcgacatcg tgccccgcgg caaccgtcgg gtctacgaca tgcgcacggt
      421 gctcgacgtg gtgttcgacg cgcgtggctg gtttgaggtg cagccgaagt tcgggcccgc
      481 gatcatctgc gctcttgccc atctcggtgg aaatccagtc gcggtggtcg ccaaccagcc
      541 acaggtgttg gccggttcca ttgacgccga cgccgccgac aaggccgcgc atttcatcac
      601 ggtggccgac tcgttccatc tgccgatcgt ctttcttgcc gacaacccgg ggatgttgcc
      661 cggcagccgt tccgagaaga gcggggtgct gcgcagcggt gcgcgcatgt tcgctgcaca
      721 gacggcggcg acaacactca aactgcatgt aacgctgcga aaggcgtacg gcttcggctc
      781 aatggtgatg tcgctgttgg gattcgacaa ccagagcgcg acgttcgcat tcccgggcgc
      841 gacgatggga gcgatgagcg ccgccgccct gagtaaggcc tcacatgccg ccgacgacgt
      901 cgaagccgag ctgcgaaaga tggaggtgga ggcgtcgttc cggtctgcga gccatctcgg
      961 cttcgacgac ctcatccacc ccgaggaaac ccgcaacgcg ctgctgtcgg cgctgcagcg
     1021 tggcctctac agccggcaag cggcggcgga accggtgagc cgcaccgtca tcacgcccta
     1081 agttctcagc gcgagcagac gcaaaagtgc ccaattccgc gatgttttag gcagttttac
     1141 gtctgctcgc ggtaggaact agagccgctt gaggcgacga accaccttgt tgaacggaag
     1201 catggtcgca agtgcaccac ggatattcgg ctgcatcgga ccgaagttga ccacgacgat
     1261 gtaacgcaca ccatggtccc gccacaccgc cgcctgctcc agaacgtcgt cgaccgtgcc
     1321 gttcaggatc atgccgttga ccacggaaag cggaacctga gaagactttt cgcggatggt
     1381 ccgctcgtcc atcgtgaatg ccacgtggtc ctgcatcccg gcgaaatcgg cgcccatcgg
     1441 atgggcggca ccatggtgcg tgtagaactc cgctggcccg ttgagcgccc aagtcttcac
     1501 ggcggtcgtc tcgagggcgg catccacatc gtcgcggttc gacgcggcga ccaccggcat
     1561 ccagaaggcc ggtgtgatcg ccatcgggtc gcggcctgcg tcggaggcgg cgcttcggat
     1621 gatctccagc cgcttggcgt agtcctccgg tcggtgggcg aatccgggga accatgcgtc
     1681 gccgtaacgc ccggtggcac gcagcattcg agggccgtgc gcggcgatcc agatctctgg
     1741 ccatttgccc ttgtacggcg ggagatcgaa catcgcatcg cgcaacggat agaacggcga
     1801 atcacggttg acgagttcgc ccttcgaatc ccacagggcg cggatcgtgg ccagggcctc
     1861 ttcgaaccgg gcaacaggtt tcgaccagtc gacgccgtag ggttcgttgc cggcacgctc
     1921 tcctgggcca atgcccagaa tcgctcgccc ccgcgtcatc aagtgcagtg tcgccgcagc
     1981 ctgtgccgtc accgccggat ggcgcttacc ggtgtccgtg accccaacgc ctagccggac
     2041 gcggttgaat ctcctgcgtg ccgcgaggct gccgagcagt gtccacgcct ccatcgcggc
     2101 gtccggtgat ctgagcagcc gcgcggcggt ggtgtacttc ttatcccaca acggccgcgg
     2161 cgtctgaccg ttgatgtggt cgggaaccca ataggagtcg acgcgattgg caacggctgc
     2221 aacgtggctc gcacgactgt aggtgtcgac ggtcggtcgc ccggccagga tcgagtccat
     2281 taggccaagg cggaatcggg acactacggg ctcgcgcggt acgcgtcgac gatcggctcc
     2341 agctcgtccg gagtcgcgcc gtgcatgatc aaagcgtcgg ctccatagtc gaactccttg
     2401 cggatcctgt cgacacactg ctgggccgat ccggttgccg acggctccag ccactcgtcc
     2461 gggatcaaag tggcgatgtg ttcgatctgc tccggtgtcg ccttgtggtc gatgccgcca
     2521 gggatcgagg tcacgaccga gtcctcccga aaccgttgca gcacagcggg gtcccagccg
     2581 ttggtgttga ccagcaggtc gccgtagccc tgtaggtagg tcgccagtcg ggcgacggtt
     2641 ttcttcagcc gcagttcctc cggcaggtgg tcgccgactg tggcgaagca cgaccacacc
     2701 cgcacgctgt cagggtcacg gccggcttgc tctgcggcat ccttaacagt cttgacgcag
     2761 cgctgcagag tctccggcgt gaagtaggtg tgcaggatga cgtcgtcgaa cacgcgtccc
     2821 cccaaggcca acgtccgtgg cccgaacgcg acgatggcca gccggatgtc ttccttgaag
     2881 tcggggtcga ggaacagcac ctgatacttg ccgatcggtc cgtcgtggtt gaagatcacc
     2941 tcgccgttcc agagccgacg catgacctgc gcgaagtcct ccatctgtgc agtcgtcacg
     3001 gccgggatac cgaaggcgcc gtacatcgcc gccacgccgc gcccgatgcc caatgtgaat
     3061 cgtcctccgg acaggcggtg catggtagtg gcccacgacc ccgtgatcag cggatggcgc
     3121 gtgttgtgat tggttgcggc cgttgcgatc tgcatccgat tggtgaccgc gcacgcggcg
     3181 cccacgagcg acgaggcctc cttgacgttc caacgctcgg agatgaacgc cgtgccgaag
     3241 cccagctcct caccgcgacg ggcctcgtcc atcaacgtcg ccggaccctc accgccggct
     3301 ccagccagta ggtagtaacc cagctcatcg agcacgcgtt cggtcacgtc caaactcctt
     3361 gtttgtaacc gcaattccac acctcggtga tcctgccgtc gatcacgcga aagatctcca
     3421 tgctgccaac ggtggtgtcg gttccgtcct tcagctttat cggcgactcg tagacgatcg
     3481 ccacgtgttc cccgtcgtcc ccggcgacca cgaggttcag atcaaatcgg agttcctcgg
     3541 acacttccca catgtcgacg atgcgttgca ccgcctgctc atgggtgagc gttagcgtgt
     3601 cgcccacctc gtggcggatc accgaatcgc cgatcaactc ctcagcgagc gcgaaatcac
     3661 gctcgttcca aaccactaag ttgtactgct cgaccacttc acgagccgtc cgcgtcatcc
     3721 gaacacctcc agtgcgtcga tgtcctcgat ggtcgccacc gcccgttcgg tgagcttcag
     3781 gcacagcgcg cgggaacgtt caggcaaggc atcccagccg atcaggatga tgactggcaa
     3841 cagcatcagg taggccacgg cgaatcgata gtggcgccac gcgtcgtcga acgagtagtc
     3901 ggatatgccg tgctcggaga gctgttgcag atactcgcgg accaactgtt gatcgtggcc
     3961 acggcgcgcc tccgtcgga
//
LOCUS       DASRJA010000427        10000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1445680,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000427 DASRJA010000000
VERSION     DASRJA010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10000
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445680"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VFC01_18590"
     CDS             complement(<1..283)
                     /locus_tag="VFC01_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZN81671.1"
                     /translation="MADGDRGSGRNGSVRRLVIVESPTKARKIASYLGSNYIVESSRG
                     HIRDLPRNAADVPAKYKSEPWARLGVNVEHNFEPLYIISPDKKSTVAELK"
     gene            complement(420..998)
                     /locus_tag="VFC01_18595"
     CDS             complement(420..998)
                     /locus_tag="VFC01_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81672.1"
                     /translation="MSQLSFFSAESVPPAVADLTGILAAPGQVVLVGTGARLSVVVDR
                     LWRAEGLAEMISEAGLQPEITHTDENTPLVRTTADPRLLAIARDWTTGAVKTVPPQWL
                     PGPRELRAWTLAAGTTEADRYLLGLDPHAPDTHAPLASAMMRVGIAPTLIGTRGARPA
                     LRISGRRRLSRLVENVGEPPGDVEAFEQWPRI"
     gene            complement(1120..1323)
                     /locus_tag="VFC01_18600"
     CDS             complement(1120..1323)
                     /locus_tag="VFC01_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZN81673.1"
                     /translation="MPQGTVKWFNAEKGFGFIAPEDGSADVFVHYTEIQGSGFRTLEE
                     NQKVEFEVGQSPKGPQATGVRAV"
     gene            1600..3930
                     /locus_tag="VFC01_18605"
     CDS             1600..3930
                     /locus_tag="VFC01_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN81674.1"
                     /translation="MSSPGSDFGRALLACAIDGTDPDEQPVRHVADLPARQGRPQAWP
                     LWADPDVVRAFVDHGIEAMWSHQFAVANLAHDGRHVVLSTGTASGKSLAYQLPILTAL
                     KENPRARALYLSPTKALGHDQLRSAVALTEAVPGLANVAPSSYDGDSPGEVRRFARER
                     SRWLFSNPDMIHLSLLRNHARWAVFLRNLRFIVVDECHYYRGIFGSNVAMVLRRLLRL
                     CTRYSSAPTVVFASATTAQPAATASELIGQTVTEVTDDGSPHGARTVALWEPALRDDI
                     VGENGAPVRRSAGAEAARVMADLIAEGARTLTFVRSRRGAELTALGARARLDDIAPEL
                     AERVASYRAGYLAEDRRALERALAEGELRGMATTNALELGVDIAGLDAVVLAGFPGTI
                     TSFWQQAGRSGRRGQSALIVLIARDDPLDTYLVHHPAALLDKPIERVVIDPANPYVLG
                     PQLLCAATELPLTDAEVRMWNAEAVAGALVDDGLLRRRTSGYFPSPGVDPHPAVDIRG
                     SSGGQIAILEADTGRLLGSAGVGQASASVHPGAVYLHQGESYVVDTLDFEDGVAFVHA
                     EDPGYTTFARELTDIVVTGQGERNTYGPVTVGLVPVSVTNTVIGYLRRRLNGEVIDFV
                     ELDMPTRTLETTAVMCTITPEALQHNGIDLLRVPGALHAAEHAAIGLLPLVASCDRGD
                     IGGVSTAVGPVGGLPTIFVYDGYPGGAGFADRGFRKLDTWWAATAAAIEACECPLGCP
                     SCVQSPKCGSGNDPLDKGGAVRVLRLVLGALAGQTS"
     gene            4229..5314
                     /locus_tag="VFC01_18610"
     CDS             4229..5314
                     /locus_tag="VFC01_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZN81675.1"
                     /translation="MNHDWLLVETLGSEPAVVAQGHRTKNLVPISAFLRRNPHLMAIQ
                     TAIGETVRAGVGLSSITPKNDRVIRTDIVKMSDGLIHGVHLWIGPPDMDPPERPVPGP
                     LKWDLTRGVATDTPESLFNSGRDPEKEATQGRAFAEDLPMRDLNPSEAKVLTMLMRPE
                     PGMTFCTNWDVTDYHGNPITVGWVARAQMEAEDDGTQRLICRAMNWRSEHESSADPRS
                     DLAARILNGLAQSGVHRVLVDLHHWKLLKWLDEPAPFFNWRAREAGTPIVHPDDAPQM
                     AKMTIEFGSGRTSGILRLPGNGGGWVPVHMTINRVELDKDTYAGLVALRLPTADELTA
                     TDIKAVDVTTAAEKRKGRSRKDKSSKS"
     gene            complement(5304..7025)
                     /locus_tag="VFC01_18615"
     CDS             complement(5304..7025)
                     /locus_tag="VFC01_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006551065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN81676.1"
                     /translation="MNDGGGAGLVSGETADSHEVFPRLTADQLNVLMTWGQRRTAEVG
                     DFLYRAGERVDEVLAVLSGRVALVQSDPDGDHAVEVHRQGRVLGELGMLEGQPAMFSA
                     RMVEAGQIVAVPTAHLRDVTLRDSVLGETILRAFLIRRARLIERGAGMRIIGSSYAPD
                     TRRLLDFATRNRLPYKWIDHEKEPDVDAFLRRMNVARTDTPIVILRTGKLLRNPSNAQ
                     VAAELGILVTPSPIGLCDLLVVGAGPAGLAASVYGASDGLSVTTVDAIAVGGQASTTS
                     RIENYVGFPAGISGAELTERAVLQARRFGVRLVVPAKVTALALDEGHFRVELGSQDVV
                     RARAVVVASGARYRRLDVPGLARFETANVYYEATINERQACGGQPVAVVGGGNSAGQA
                     AVYLAATTRTVYLLVRDRSLGAKMSQYLADQIESDPRITVMTHTEIRELHGDQFLTAI
                     EVEDATTGLRKDLAVRALFVFIGAEPQSSWLAGAVQLDRNGFVLTGTDLDGSSDQTPH
                     KHRSILETSQPGIFAAGDVRRGATRRVAAAMGEGAIAVALVNQYLANAGHAVRHANAV
                     PVSLVRT"
     gene            complement(7022..7369)
                     /locus_tag="VFC01_18620"
     CDS             complement(7022..7369)
                     /locus_tag="VFC01_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HZN81677.1"
                     /translation="MKYQGIDTTVPPTGAGCGECEQQHGWWLHLRRCAQCGHIGCCDN
                     SPGQHATAHAEATGHPWIRSYEPEEHWFYDYREQQFYDDGPQLAPPDHHPLDQPTPGP
                     AGRVPANWQELLH"
     gene            complement(7381..8022)
                     /locus_tag="VFC01_18625"
     CDS             complement(7381..8022)
                     /locus_tag="VFC01_18625"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HZN81678.1"
                     /translation="MSAVDYRGANQTRTLFGRVMWYVAATAGFFALGTYVGRGLGYGW
                     GFVFFIVAFGCMIGMQFAVRSSPQLSVALLFGLGLSLGLAVSPTVIYYASADPQALWQ
                     AGGATALFVAGCGAFGYATRTDLSGVARLSFWALLALILFGVVQIFVHIPAGAVIYSI
                     LGLLIFAGLTMADFQRLRRSTDLNSAPLIAASIFLDVVNVFQLFLPLFGRSRD"
     gene            complement(8106..8297)
                     /locus_tag="VFC01_18630"
     CDS             complement(8106..8297)
                     /locus_tag="VFC01_18630"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HZN81679.1"
                     /translation="MSTTNAAVLRKRGSVWADLSERVPADADQDEIAAYYDRGILTVS
                     VELSQPKLAENRIPVQSAS"
     gene            complement(8557..8877)
                     /locus_tag="VFC01_18635"
     CDS             complement(8557..8877)
                     /locus_tag="VFC01_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81680.1"
                     /translation="MEFGLSSSAGRLKDVHLSSHHRDTLGKIFSHPASGNVEWRDVLS
                     LLEYVGTTTEERNGKFRVTLGPETEVLEAPRGKDIDEQLIVDLRRMLRQAGLTPDDGT
                     ADTG"
     gene            complement(9019..10000)
                     /locus_tag="VFC01_18640"
                     /pseudo
     CDS             complement(9019..10000)
                     /locus_tag="VFC01_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304495.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1797 a         3241 c         3212 g         1750 t
ORIGIN      
        1 ccttcagctc ggcgacggtg ctcttcttat ccgggctgat gatgtagaga ggctcgaagt
       61 tgtgctcgac gttcacgccg aggcgcgccc acggctcgga cttgtacttc gcgggcacgt
      121 cggcggcgtt gcgcggcaga tcgcggatgt gtccgcggga cgactcgacg atgtagttgg
      181 agcccaggta gctagctatt ttgcgtgcct tagtaggcga ctcgacaatg acgagccgcc
      241 gcacgcttcc gttgcggccg ctgccgcggt ccccgtcagc cacctctgtc tacgctccaa
      301 ttctcctgtt gccaccgagc gccggatgta cgcctcgacg gtccgtccgg ccctgctggc
      361 aactgacaat ttcgcacccc gcgccagccg acgcaaactg accccccggt ttcagcccgt
      421 catatgcgag gccattgttc aaacgcctcg acgtcgcccg ggggttcccc cacgttctct
      481 accaggcgcg atagccgccg tcgcccactg atccgcagcg cggggcgtgc ccctctggtg
      541 ccgatcaacg tcggcgctat ccccacccgc atcatcgccg aagcgagggg tgcatgagtg
      601 tccggggcgt gcgggtccag gcccagcaag tagcgatcgg cttcggttgt gcccgccgcg
      661 agcgtccagg cgcgcagttc gcggggcccc ggcagccact gcggcggcac tgtcttgacc
      721 gcgccggtgg tccagtcgcg ggcgatcgcc aacagccgcg ggtccgccgt ggtgcgcacc
      781 agcggggtgt tctcgtcggt gtgggtgatc tccggctgca gcccggcctc gctgatcatc
      841 tccgcgaggc cctcggcacg ccacagccga tccacgacca cggacagccg tgcgccagtc
      901 ccgaccagca ccacctggcc gggcgcggcg aggatcccgg tcaggtcggc gaccgcgggg
      961 ggcaccgact ctgccgagaa gaacgacagc tggctcacgt aaccgacagt aggccagcag
     1021 tcccggacga atgcgtcgcc gggccgcagg ggcccttaaa cgaaaacacc ccgcgccgtc
     1081 cgatgcggat gacgcggggg gttattcagg ttcgattgct cagacggcgc gaacacccgt
     1141 ggcctgcggc cccttggggc tctggccgac ctcgaactca accttctggt tctcctccag
     1201 ggtgcggaat ccgctgccct gaatttccgt gtagtggacg aacacgtccg cagagccgtc
     1261 ctccggggca atgaagccaa agcccttctc cgcgttgaac cacttcacag ttccctgtgg
     1321 catttcctgt tgttccttct cttattcttc cgggtgcggt ccaccgtgtt tcggtacacc
     1381 gggcccgttc cgaccgctta ccttcgcggt gtcggcggaa cttgacccga cctacaaccc
     1441 tcgcaggaac cgcgatcgca acgtcgttcc tgcgagtgct agtacacgaa cacagaagct
     1501 gcgaccgcgc taagtcaatc atgttcgggg ctgacgcgac agtctcggcg ggatcaagtg
     1561 gggaacaatt ctcttacgta gcggtttcag gaggcagcag tgtcaagtcc ggggtcggat
     1621 ttcggccgcg cgctgctcgc ctgtgcgatc gacggcaccg atccagacga gcagccagtg
     1681 cggcacgtcg cagacctgcc agcacgtcaa ggccggcctc aggcttggcc gctgtgggct
     1741 gacccagatg tcgtgcgggc gttcgttgat cacggtatcg aggcgatgtg gtcgcatcag
     1801 ttcgcggtcg ctaacctcgc gcacgacggt cgccacgtgg tactcagcac cggcacggcg
     1861 tcgggcaagt cgctggccta ccagttgccg atattgacgg cgctgaaaga gaatccgcgt
     1921 gcgcgcgccc tgtatctgtc cccgaccaag gcgctcgggc acgaccaact ccgctctgcg
     1981 gtggcactta ccgaggccgt gccagggttg gccaatgtcg cgccgagttc gtatgacggt
     2041 gacagtcccg gcgaggtgcg gcgtttcgct cgcgagcggt cgcggtggct cttctccaat
     2101 cccgacatga ttcacctgtc gctgctgcgc aaccacgctc gctgggcggt gtttctccgc
     2161 aacctgcggt tcatcgtcgt cgacgaatgt cattactacc gagggatttt cggctcgaac
     2221 gtggcgatgg tgctgcgccg cctgctgcgg ctgtgcaccc gctactcgtc ggctcctacg
     2281 gtggtcttcg ccagtgccac cactgcacag cctgcggcaa ccgcatcaga gctcatcggg
     2341 caaaccgtca ccgaggtcac cgacgacggc tccccgcacg gggcacgcac ggtcgcgctg
     2401 tgggagcccg cgctgcgtga tgacatcgtc ggcgaaaacg gtgctcccgt gcgacgttcg
     2461 gccggcgccg aggctgccag ggtgatggcc gacctgattg ctgagggcgc acgaacgctg
     2521 acgttcgtgc ggtcgcgccg cggcgcggag ctgactgcac tgggtgcgcg cgcacgtctt
     2581 gacgacatag caccggagct ggccgagcgg gtggcgtcat accgggcggg ctatctggcc
     2641 gaggaccgtc gcgcgctgga acgtgctctg gcggaaggcg aattgcgcgg catggccacc
     2701 accaacgcgc tggagttggg tgtcgatatc gccggcttgg atgcggtggt tcttgcaggt
     2761 tttcccggca cgatcacatc gttctggcag caggccggcc gttcgggccg tcgcgggcag
     2821 agcgcgttga tcgtgctaat cgcgcgcgac gacccgctgg acacgtatct cgtccatcat
     2881 ccggctgcgt tgctggacaa gccgatcgaa cgcgtagtga tcgacccggc caacccgtat
     2941 gttcttggac cgcaactact ttgcgcagcc accgagcttc cgctgaccga tgccgaggtg
     3001 cgaatgtgga atgccgaagc ggtggcgggt gcactggtag acgacggcct gctgcgccga
     3061 cggaccagcg gctacttccc cagccccggt gtcgatccgc atcccgcggt ggacattcgc
     3121 ggttcgtccg gcggccagat cgcgattctg gaggccgata ccggtcgact gctcggcagc
     3181 gcgggagtgg gccaggcctc ggcgtcggtt caccccggcg cggtgtattt gcatcagggt
     3241 gagagttatg tcgtcgacac tctggatttc gaggacgggg tcgcgttcgt gcacgccgag
     3301 gatcccggct acacgacgtt cgcgcgcgag ctcaccgaca tcgtggtgac cggccagggt
     3361 gagcgaaaca cgtacgggcc ggttaccgtt ggccttgtgc cggtgtcggt aaccaacacg
     3421 gtgatcggat atctgcggcg ccggttaaac ggtgaggtga tcgacttcgt cgaactcgac
     3481 atgccgaccc ggacactgga gaccactgcg gtgatgtgca ctatcacgcc ggaggcgtta
     3541 cagcacaacg gaatcgattt gctgcgcgtt cctggcgcat tgcacgccgc agagcatgcg
     3601 gcgattggtc tgctgccgtt ggtggccagc tgcgatcgcg gcgacatcgg cggcgtctcg
     3661 acggcggtgg gtccagtagg gggactgccg accatcttcg tctacgacgg ctaccccggc
     3721 ggggcgggct tcgccgaccg cggcttccgc aagctcgaca cgtggtgggc cgcgacggcg
     3781 gcggcaatag aggcctgcga atgccccctt ggctgcccgt cctgcgtgca gtcgccgaag
     3841 tgtggcagcg gcaatgaccc gctcgacaag gggggtgcgg tgcgagtgct gcgtctggtt
     3901 ctcggcgcgc tcgccggtca gacgtcgtaa tttacccatg gtggcgaccg acccctcgac
     3961 ggtcgccccc catggatccg tggccaggcg atgctgtgat cgagcacacc aaacacacag
     4021 acaggtgcgg cgaacggtcg gacaacgcga tccgttccca atcccggaac tcgggcgcag
     4081 acggaaagta cctcgtcgga ggggttatcg caacttagga ctgctttacc gaagttggtc
     4141 cacaaactgt ctgccgcgac gaatatacgg attccgtagc ggacttcggc tacaaggctg
     4201 cggttagctc gacggtaaga tgcccgccat gaaccacgac tggctgctcg tggagacact
     4261 gggcagcgag cccgccgtgg tcgcacaagg tcatcgaacc aagaacctcg ttccaatcag
     4321 cgcattcttg aggcgaaacc cccacctgat ggcgatccaa acggccatcg gcgaaaccgt
     4381 gcgcgccgga gtcggcttga gcagcatcac gccgaagaac gaccgcgtaa ttcgtactga
     4441 cattgtcaag atgtccgacg ggttaataca cggtgtacat ctgtggatag ggccgccgga
     4501 catggatccc cccgagcgac cggtgcccgg acccctgaaa tgggacctca cccgtggggt
     4561 cgccaccgat actccggagt cgctgttcaa cagcgggcgg gatcccgaga aagaagccac
     4621 ccagggccgg gcgttcgccg aggacctgcc gatgcgcgat ctcaatccca gtgaagccaa
     4681 ggtgctcacg atgctgatga ggcccgagcc cggcatgacg ttctgcacca attgggacgt
     4741 caccgactac cacggtaacc ccatcaccgt tgggtgggtc gcccgcgcgc agatggaggc
     4801 tgaggacgac gggacgcagc gactgatctg ccgcgcgatg aactggcgca gcgagcatga
     4861 gtcgtcggcc gatccgcgta gcgacctcgc agcgcgaatc ctcaacgggt tggcgcaatc
     4921 tggggtgcac agggttctgg tcgaccttca tcactggaag ctgttgaagt ggcttgacga
     4981 gccggccccg ttcttcaact ggcgcgccag ggaggccggc acgccaatcg tccatccaga
     5041 cgacgcaccg cagatggcaa aaatgacaat cgagttcggc tcaggaagga cctcaggcat
     5101 attgcggttg ccgggcaacg gtggcggatg ggttccggtg cacatgacca tcaaccgcgt
     5161 cgagttggac aaggacacct acgccggact ggtggctctg cgtctcccca ctgctgacga
     5221 gctcaccgcc acggacatca aagcggtgga cgtgacgacc gctgcggaaa agcgaaaagg
     5281 ccggtcgcgc aaggacaaat cgtctaagtc ctaaccagcg acaccgggac agcgttagcg
     5341 tggcgcacag cgtgcccggc gttcgcgaga tattggttga ccagcgcaac cgcgatggcg
     5401 ccctcaccca ttgccgccgc cacacgtcga gttgcaccgc gacggacgtc tccagccgcg
     5461 aagatgcccg gctggctggt ttccaaaatc gaccggtgct tgtgcggtgt ttgatcggat
     5521 gacccgtcga gatcggtgcc ggtcaacacg aaaccgttcc ggtcgagctg gacggcgccg
     5581 gcgagccaag atgactgcgg ctcggcaccg atgaacacga acaaggcgcg taccgccagg
     5641 tccttgcgta accccgtcgt ggcgtcttca acctcgatgg cggtcagaaa ctggtcgccg
     5701 tgtagttcac ggatctcggt gtgcgtcatc accgtgatgc gggggtcgct ttcaatctgg
     5761 tcggccaggt attgggacat cttggcgccg agactgcggt cacgcaccaa cagatacacg
     5821 gtgcgggtgg tggcggccag gtagaccgct gcctgcccgg ccgaattgcc cccgccgacc
     5881 actgccacgg gttgcccgcc gcacgcctga cgctcgttga tcgtggcctc gtaatacacg
     5941 ttggcggtct cgaatcgggc aagtccgggc acgtcgagcc gccgataacg cgctcccgat
     6001 gcgacgacga ccgcgcgggc acgcaccacg tcctggcttc ccaattccac ccggaagtga
     6061 ccctcgtcga gcgctaacgc ggtcactttc gcaggcacga ccagccggac accgaacctg
     6121 cgggcctgga ggaccgcgcg ctcggtcaat tcggcgccgg aaatgccggc gggaaaaccc
     6181 acatagttct ctatccggga ggtggtactg gcctgtccgc cgacggcgat ggcgtcgacg
     6241 gtagtgacgc tgagaccatc cgacgcgccg tacacgctgg cggcgagccc ggcggggccc
     6301 gccccgacga cgagcagatc acacaacccg attgggctcg gcgtcaccaa aatgcccagt
     6361 tcggcggcga cttgggcatt cgacgggttg cgcagcagct tcccggttcg aagaatcact
     6421 atcggtgtgt cggtgcgagc cacgttcatt cttcgcaaga aggcatcgac atctggttct
     6481 ttttcgtgat caatccactt gtagggcaag cggtttcgcg tagcgaagtc caacaggcgg
     6541 cgagtgtccg gggcgtagga cgagccgatg atgcgcatcc ccgctccgcg ttcgatcaag
     6601 cgcgcacggc ggatcagaaa agcccgcagg atcgtctccc ccaacaccga atcccgtagg
     6661 gtgacgtccc tgaggtgagc agttggcacc gccacgatct gaccggcctc caccattcgt
     6721 gcgctgaaca tcgccggctg cccctccagc atgcctaact cgccgaggac gcgtccttgt
     6781 cggtgaacct caacggcgtg gtcaccatcc gggtcactct gaacaagcgc aacgcgcccg
     6841 ctcaataccg ccaagacctc gtcgacacgc tctcccgcac ggtaaagaaa atcgcccact
     6901 tctgcggttc gtcgctgccc ccaggtcatt aggacgttca gttgatcagc ggtcaaacga
     6961 gggaaaacct catggctgtc cgccgtttca cccgaaacca gcccggcacc gccgccgtca
     7021 ttcaatgcag cagctcttgc cagttggcag ggacgcgtcc ggcgggtcct ggcgtcggct
     7081 gatccagcgg gtgatggtcc ggcggcgcta gttgggggcc gtcgtcgtag aactgctgct
     7141 cccggtagtc gtagaaccaa tgttcttcgg gctcataact tctgatccag ggatgtccgg
     7201 tcgcttcggc gtgggcggtg gcgtgttggc cgggcgagtt gtcgcagcac ccgatgtggc
     7261 cgcattgcgc gcaccggcgc aggtgcaacc accatccatg ctgttgttca cactccccgc
     7321 agcccgcccc ggtgggcggg acagtggtgt cgatgccctg atatttcatc gtgcggctac
     7381 ctaatctcgg cttctgccga ataacggcaa gaagagttgg aacacgttga ccacgtcgag
     7441 gaagatcgac gcggcgatga gcggggcgga attcagatcc gtcgatcgcc gcagccgctg
     7501 aaagtcggcc atggtcagac cggcaaaaat gagcaacccc aggatcgaat agatgaccgc
     7561 cccggccgga atgtgcacga agatctgcac gacgccgaac aggatgagcg cgagcagtgc
     7621 ccagaaagag agccgcgcga cgcctgacag atccgtgcgg gtggcatagc cgaaggcgcc
     7681 gcagccggcg acgaacagtg ccgtcgcacc gcccgcctgc cacaacgctt gcggatcggc
     7741 gctggcgtaa tagatcacag tgggcgatac cgccaacccg agtgacaggc ccaacccgaa
     7801 cagcagggcg acgctcagct gaggtgagct gcgcacggcg aactgcatcc cgatcatgca
     7861 cccgaacgcc acgatgaaaa acacgaaccc ccagccgtac ccgaggccac ggccgacgta
     7921 ggtacccaac gcgaagaagc cggctgtggc agccacgtac cacatcaccc gaccaaacaa
     7981 tgtgcgggtc tgattcgccc cgcggtaatc cactgcgctc atggcttgcg ctccaatcag
     8041 tttcggcgtc agcgctatgt ggagcggtcc cagaacgccg ttgttcgggg accgctgcca
     8101 actcgtcagc tggccgattg caccgggatg cggttctcgg ccagcttggg ctgtgacaat
     8161 tcgacggaga ccgtgaggat gcccctgtcg tagtacgcgg cgatttcgtc ctggtcggcg
     8221 tcggcgggta cacgctccga cagatcggcc cacactgagc ctcgcttgcg tagaacggca
     8281 gcgttggtcg tactcatcat tctccttcag cgacgacgcg ccgacgttaa gccttgcccg
     8341 gtcaacgaca ttgggggcac tccagaccta gcgccggcaa ggattgcctg acagctccat
     8401 cgtctgacca actcgccgcc agcgcgctat ccgccgtgac aagcccacac gccaattggt
     8461 gtcaccgttg acatcgaggt cgtatcggtt ctggtgcact gctgcccggc gctggttggc
     8521 tcgccccacc gcccgttccc gcgatcgcgg cggcgttcac ccagtgtcgg cagttccatc
     8581 atccggtgta aggcccgcct gcctgagcat ccggcgcagg tccacgatca actgctcatc
     8641 gatgtctttc ccacgcggcg cctcgagcac ttcggtttcg ggacccaacg tcactctgaa
     8701 tttgccgttg cgctcttcgg tcgtcgttcc gacgtactcg agcaacgaca agacgtcacg
     8761 ccactcgaca ttgccgctgg ctggatggct gaagatcttc ccgagagtgt cccggtgatg
     8821 actacttaag tgcacgtctt tgagccgtcc tgccgaactg gatagaccaa actccatcgt
     8881 gtcacatccc cacacccagt ctgctggcag acctcaaaat ccttcggatg aaaccgcgca
     8941 caattgctcc gtacggtgaa tccgttgtcg ccgttgaagt tcgagtcgct agggcgtgtg
     9001 tcgaagcctc tcgaaggctt aatcgtcgct gtcgtcggcg gtgcgcccgg tgttgaccca
     9061 tgatgcggcc gcaccgatga ccatcatcac cgcggcggcg gtgaacaccg cgaccaaccc
     9121 gttgtggaaa ggccccgaga tcaactgcgg gaagtatcgt cttccggtca ggacggccgc
     9181 gttgactccg ggctgttgca ggactccgga ggaggccagc agttcggcga tcgggttgta
     9241 ccccaagaac gccgcgaaca agctttcggc cggaggcagg ttggccgcgt tgttggctac
     9301 cgtcgcgggt acgccttgct gctgcagccc ggcgaggagc gtggtgggca gcgagtgggc
     9361 caggccggcg atcatcagcg agaagaaaat gccgatcgat aatgacatgc cggcgttggt
     9421 gaccgtcgca cggatacccg aggcggcgcc gcgctcggcg gctggcacgc tggacatgat
     9481 cgccgcggtg ttaggagcgg tgaagatgcc gccgcccaag gcaagcgtga cgagcgcgac
     9541 aatggcgagc gcgatcgaac ggaggaccca ggggttggtc caaccggtgg gatgaccgcg
     9601 gtagggctgg atcccgtagg tgattgctgc cagcagtgcg gtcaggcccc cgccgaaaag
     9661 cactgtgccg ggccaatcga ggcgagcgca gtccgccgat cagcagaccc agaactggcc
     9721 ggcgatggcg gcgacctggt tgaccccgag cgccatgccg cgctgccggc tggggaacgc
     9781 gtcggtcagg atggctgccg aacaggccat cagcatcgcc ccgccgatgc ccttgatgac
     9841 gcgccagctg ataagccata ccgcgccgcc gccgaggcga aacgggtcga acgacaacgc
     9901 aatcgcggcg attgtgaaga tcgcgaaacc taggttgtag atgcgcaccc ggccgaagat
     9961 gtcgccgagg cgcccgagcg ggaggacgag caccgcggtc
//
LOCUS       DASRJA010000428        22382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4006978,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000428 DASRJA010000000
VERSION     DASRJA010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22382
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4006978"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFC01_18645"
     CDS             <1..239
                     /locus_tag="VFC01_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81681.1"
                     /translation="GFSAVGLGAGIANAAPPAPVVAGTLWQQDRDHGGGGYWGHGDGR
                     GWDDRGGWGYGGNYGYGPGWGCITGPFGHITWCP"
     gene            complement(470..2419)
                     /locus_tag="VFC01_18650"
     CDS             complement(470..2419)
                     /locus_tag="VFC01_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018162755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZN81682.1"
                     /translation="MELSPRQRTALTSICDTFLPAANGAPSASELGVVDALLGAVAAN
                     PREAERRQLTQLLGLWDTPLLTALGGGGLRRFSTLGQAERERVLLSWCDSRLPQRRAA
                     FQALRKGALLVSYMLPNPDGSPNPVWEAIGYPGPLGKKEGAPPKPLKPLEIAADTDLL
                     CDVVIVGSGAGGGTAAGVLAAAGLDVVVLEMGGYYDDADFDGSEVTALTGLYLGAPQA
                     THDQSVGLLAGSCLGGGTVVNYSTSFRTPNDVREEWAGHGVPAFAGEEYTRSLDAMVE
                     RLGVNLKHNKPSRRDEIMRAGCDALGWLIDAMPRNVRGCDQGKECGYCCFGCRIGAKQ
                     SVTKTWLADAAAAGARLVINTCAERVIVVNGAARGVEARTLDGHRVTVRSRAVVAACG
                     ALHTPALLRRSGLDNTNIGKHLKLHPVFPVMATYEDEVRPWEGVMQALYSDQFRDLDD
                     GYGVKFETASIQPHIAVSFLPWRGARAHRELMEALPYSAGIAVLLRDRDGGEVRVGRD
                     GQPVVRYQLSKYDLGHLRTGLDGAAQIHEAAGAKRIFSSQNRWVAYEPGRDGDRARFM
                     ADCDAAGYGPGQCVLGSFHIMASARMGGSPATSACDPTGQTWDVRDLMVCDGSAFPTA
                     SGVNPMISIEAIAHMNARGLAARFA"
     gene            complement(2448..3515)
                     /locus_tag="VFC01_18655"
     CDS             complement(2448..3515)
                     /locus_tag="VFC01_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN81683.1"
                     /translation="MAQLPETRYAKGPGGDIAYQVVGDGPMDLVVVPGWFSHVDLLWN
                     HPGWASFIGDFASFSRVILYDKLGTGLSDPVDAVPTLESRVDDLRAVMDAADSQRAAL
                     FGLSEGGPIGMLFAATYPKRVQALILYGTYACGTGEDDGSPGRAKWLKLMNAIRPTLD
                     HWGGGHTVDWAAPSLQPSAPYRTGMGALERAGMSPKMARLTFEAVLTQVDVRDILGND
                     EITREEISRFQGREVKHTGDGFLAAFDGPTRAVRCATTLTERMPELGIDIRTGLHAGE
                     CEVRGEDIGGIAVHIGARIAALARGREVLVSSTVKDLVNGSGITFEDRGTHVLKGIPA
                     QWQLYAPAGEHDPAGGIFGRN"
     gene            3601..4074
                     /locus_tag="VFC01_18660"
     CDS             3601..4074
                     /locus_tag="VFC01_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZN81684.1"
                     /translation="MTLNQFTATRASGAVVLIRLYVGLIFAGEGVLKFVRPDALGPGR
                     FIKAGIPGGTFFAYLDGVFEIACGLLILVGLLTRLATLPMIVDMLGALGITKVPLLWG
                     HAPLYPKEGGFWDFFHEGRLEVAMLCGSVFLLIVGAGAYSFDAQLNRDRTPAVVT"
     gene            4114..4629
                     /locus_tag="VFC01_18665"
     CDS             4114..4629
                     /locus_tag="VFC01_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016893368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN81685.1"
                     /translation="MAGLQPVTGAVYPDWDAVYRDNVTWVYRLMFGKVGNQPDAEDLT
                     AEVFMAALRPLRVSATVPEVRKYLRMTARTVLASYWGDRMGRPITTIEDDVPEVIEPE
                     AVASDAPDKVRRLLQRLPDNYRRILELRFLQAFSVREAASQMGVSVANAKVLQYRALR
                     MAAQSGEEASS"
     gene            4626..5312
                     /locus_tag="VFC01_18670"
     CDS             4626..5312
                     /locus_tag="VFC01_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZN81686.1"
                     /translation="MSDRTVRRFVDRLLRGQPTERARPDDFEAEQMKTAIELRAARLG
                     SDAPREEFVTDLHRRLASEMAEAPPPGSRWAPRRRQVVIGTSVAAASAAAGLVVGRNL
                     LAPTETRQATPQGELEPNAGTWRPVGASVDLPEGGALAFDLGSVNGFVHRTDAKLQAV
                     SGVCTHQGCKLWLDAPESRLRCPCHSTSFSLAGTTVTHQLPVAPPPLPKLQVREINGV
                     IEVFAPTKPA"
     gene            5355..5858
                     /locus_tag="VFC01_18675"
     CDS             5355..5858
                     /locus_tag="VFC01_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZN81687.1"
                     /translation="MRLLLIADTHVPKRARDLPSRVWEEVEQADVVLHAGDWVEPRLL
                     DQLEARSKRLVGCWGNNDAAELRRRLPERADATLGGLRFTVVHETGSATGREARMAKD
                     YPDTDVLVFGHSHIPWDTTARTGLRLLNPGSPTDRRRQPFCTFMTATVDNAALSDVVL
                     HNLERHA"
     gene            5851..6264
                     /locus_tag="VFC01_18680"
     CDS             5851..6264
                     /locus_tag="VFC01_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81688.1"
                     /translation="MRDRPARVADVHEVAASMPHVKRIEGPKGNPIYQVGGKSFVFFR
                     TPQPDAEDPETGERYADVIMIWVESESDKLSLIQDPDSPFFTTDRFDGHPSVLVRAKR
                     LREISRVELTELIQDAWLSRASKRRADVWLADHGS"
     gene            complement(6265..6603)
                     /locus_tag="VFC01_18685"
     CDS             complement(6265..6603)
                     /locus_tag="VFC01_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN81689.1"
                     /translation="MTTLGKYTCGLDAAMAVVDGKWKPLILWELKEGPKRFNALHRSL
                     TGISQKMLTQHLKELQRHGVVHRESYHEVPPRVEYSMTPAGEELLEALGPLGDWATKH
                     IGLICAAEAG"
     gene            6693..7574
                     /locus_tag="VFC01_18690"
     CDS             6693..7574
                     /locus_tag="VFC01_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZN81690.1"
                     /translation="MTTTKQTVSFLGLGEMGSALARATISAGHPTTVWNRTAGRATAL
                     VEAGARAVSTAAEAVEASDLIVVCLFDHRSVQEVLDPIADRLAGRHLVNLTTTSPEGA
                     RELADWATGVGADYLDGGIMATPEMIGTPQSNILYSGSQLLYDDHRHLLELWGAPEYF
                     GDDAGMASLYDLALLSGMYVMFAGFFHGAAMVGAAGVPAKEFATRAAAWLQNVVFAIT
                     EYAEIIDGGDYSLPGQQSLEFSDISDIIDASRDQGINTELVDVVQRLIHRQIDAGHGR
                     DGFARIIESIKHHGATA"
     gene            7571..8431
                     /locus_tag="VFC01_18695"
     CDS             7571..8431
                     /locus_tag="VFC01_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZN81691.1"
                     /translation="MTTVSVLGLGPMGQALSTALFDANLAVTLWNRTESRADALRRRG
                     AAWAANPAEAVAATELTVVNVVDHDAVDSVLDAAGDAVADRVVVGLSSDTPERARQTA
                     KLVEDRGGRYLDGAIMSPTSTIGTPSARLLFAGPQDVFETHREVFAALGASTWLGEDH
                     GRAAAFDMSLLDLFWTSVSGFLHALTVARANGITPSELLPHAQGIVDILPPIFEEFAE
                     RIEAGRHDNSSAPVSSVAASVRHLIAASQDAGVDAGALEAFRGYVDAAVAEGYGAEES
                     SRIAAAMARR"
     gene            complement(8428..8967)
                     /locus_tag="VFC01_18700"
     CDS             complement(8428..8967)
                     /locus_tag="VFC01_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN81692.1"
                     /translation="MNVRRVVTGHNASGKSVFVSDEMVGPVTTTMMPQFQPYLLWGAD
                     KTPQFPDDGTMPEWRTYFPPIGGFRFGIFTVEPGAEQPGAADEAALADVEAKLPGLLR
                     YLDPSDPGMHTTDTIDFEVVLEGTIVLELDDGAEVTLHPGDTVVQNGTRHRWKNPSDK
                     PARLALFICGASHATVPPA"
     gene            9016..10893
                     /locus_tag="VFC01_18705"
     CDS             9016..10893
                     /locus_tag="VFC01_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HZN81693.1"
                     /translation="MDQKPPTAVIESANSEHLTHLPFEDTQDFEDTDRGFIGALEPCV
                     VKAADGRVVWDNDVYAFLDTEVPTSVHPSLWRQSQLCAKQGLYEVVEGIYQVRGLDLS
                     NISFIEGDTGVIVIDPLVSTETAAAALKLYRSHRGNRAVSAVIYTHSHVDHFGGVLGV
                     TTQADVDAGRVAVLAPEHFTEHAVQENVYAGTAMARRAAYMYGAALDRGPQGQVGCGL
                     GQVGSTGEVGLIVPTVDIRETGETHTIDGVEIEFQMAPGTEAPAEMHFYFPRFRALCM
                     AENATHNLHNLLTLRGALVRDPHGWAGYLTEAIDRFADRADVVFASHHWPTWGRDQIV
                     EFLSLQRDMYAYLHDQTLRQLNQGFTGIEIAENFQMPPALHKAWHTHGYYGSVSHNVK
                     AVYQRYMGWFDGNPGRLWAHPPEAIGPRYVDAIGGIDRVVDFARKAFEEGDFRWAATL
                     LDHAVFTDQNHAAARELYADTLEQLAYGAENAVWRNFFLAGATELREANFGTPTVAAS
                     PSIIGQLTPEQLFDTFAININGPRAWDLDVAVDIIFLDVDTNYRLTLRNGVLVYRKVP
                     ADASTANARLKVANKLRLMALAAGDNDSPGLVISGDAGALASIIGVLDRPDPNFNIVT
                     P"
     gene            complement(10883..11932)
                     /locus_tag="VFC01_18710"
     CDS             complement(10883..11932)
                     /locus_tag="VFC01_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020106412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN81694.1"
                     /translation="MGYSVVHCGTGNIGATALKAIINDPELKLVGHYVSTPEKAGRDS
                     GELGGLDAVGVTTTNDWSELVDLGADCLTYFGNSIGREADAISDLVPFLERGTNVVTF
                     SGFAIAHPATTPPEFRMPIEAACRAGNSSCFFTGIDPGWATTDLAITALAVANRIDCI
                     RVMELGWWGDYTAEFVCREYFGFGKPPGFQPLLVTGGFIETMWAPTLYQLAEVLGVEV
                     EETRVVYETDSVDHDVETGFGTVAAGTASVVHFEYQALSRGKPFAVVEHVDCVARDVG
                     KQWKQPYAPVDLAFRIEVEGDPSYAVELSFGPGGDTLSAMPVLNSIPAVCQARPGLLG
                     PLDVPRYWSRNIAKG"
     gene            complement(12028..12789)
                     /locus_tag="VFC01_18715"
     CDS             complement(12028..12789)
                     /locus_tag="VFC01_18715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN81695.1"
                     /translation="MGYADELFDLTDRVVLITGGSRGLGREMAFATASCGADVVIASR
                     KYETCVETAKEIEAATGRAALPYQVHVGRWDELDGLVDAAYERFGKVDVLINNAGMSP
                     LYESLSSVTEKLFDAVVNLNLKGPFRLSTLVAERMVADGGGSIINVSSSGSRRPRPEQ
                     LPYSAAKAGLNALTEGLALAFGPTVRVNTLMPGPFLTDISKAWDIPKTTKGAQNFALK
                     RLGNPPEIIGAVLYLASDASSYTSGSIIRVDGGIP"
     gene            complement(12801..13865)
                     /locus_tag="VFC01_18720"
     CDS             complement(12801..13865)
                     /locus_tag="VFC01_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005172196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HZN81696.1"
                     /translation="MRAFACPVCNAFAAFESDQCATCHTTLGFHLPTRSMQPVADDVA
                     QVGGQRWVRCTRSETLGCNWLAPEEQSPDERGRCLADSLIRREPDADDTLAREKLVPT
                     ARSLRRLVYQLGDIGLPIDPFWHHDGGLAFDLVSSYSTGEKVTIGHAGGVITIDLVES
                     LDDYRESLRVRLGEPYRTMLGHFRHEVGHYYQHVLVESGSGASKYLDECRRLFGDERA
                     SYSDAIARHYKFGAPDGWDATFISEYATMHPWEDFAECFAHYLHIADTIDTSREAGMV
                     LQADRVRFSAPRDIVPLESYDDVPIQRLLFDWRWISLFFNRVNTAMGKNPLYPFEIPE
                     PVVAKLGFIHRVIRETVRDQ"
     gene            13884..14492
                     /locus_tag="VFC01_18725"
     CDS             13884..14492
                     /locus_tag="VFC01_18725"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZN81697.1"
                     /translation="MQLLLIRHALPLRSEPGLGSDPDLSEEGIEQAKRLPDALARFPI
                     TRLVSSPQRRSIQTAQPVADALGLTAEIDDRLAEYDRDMEHYIPIEQIAAEFPEELAR
                     LAQGHLPSTVDETAFLDRINAGIADLVASGDHEDTIAVFTHGGVINGLLHNILATEKI
                     LCVNVDYAGVTRLLSSREGNLYVAAVNGTEHVWDLLPRNQRW"
     gene            14502..15533
                     /locus_tag="VFC01_18730"
     CDS             14502..15533
                     /locus_tag="VFC01_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZN81698.1"
                     /translation="MTTSLEGLDLDALDRHLQSVGIARSGELRAELISGGRSNLTFLV
                     FDDASKWVLRRPPLHGLTPSAHDMAREYKVVAALADTPVPVARAITMRNDDSVLGAPF
                     QMVEHVPGRVVRHDDELEKLGDNAVISDCVDALIRVLADLHAVDPEAVGLGDFGKPTG
                     YLERQVRRWGGQWELVRREEDPCDADVKRLHSALAEAIPPQSRSSIVHGDYRIDNTIL
                     DAQDATKVVAVLDWEMSTLGDPLSDAALMCVYRHPMFNIIHASSAWASPLMPEVDELA
                     NRYSQSSGQSLDNWDFYMALGYFKVAIIAAGIAFRARMGGGVAKDTDQVDKAVGPLIA
                     EGLNVIGRG"
     gene            complement(15530..15907)
                     /locus_tag="VFC01_18735"
     CDS             complement(15530..15907)
                     /locus_tag="VFC01_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HZN81699.1"
                     /translation="MTSDPAEQQPSSAALPESSRGTPPEPTATPGEYFVSPESAVKFT
                     RAAALWSALIVGFVILILLLVFILQNTDSATIHFFAWEWNLPVGVGILLAAVCGGLLT
                     VAAGSARIVQLRRAAKKNLKAGF"
     gene            16035..16976
                     /locus_tag="VFC01_18740"
     CDS             16035..16976
                     /locus_tag="VFC01_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN81700.1"
                     /translation="MSSTRRSSLAILASLLALIATACGSSNPLGGGTVSGDLKSIVVG
                     SADFPESKIIAEIWAQALEANGFTVGTQYGIGSRETYIPAVRDHSIDLIPEYTGNLLQ
                     YFDKNTKVTTPEAVELALLRVLPGDLSILTPSPAEDTDTVTVSQPTAQKWKLKSIADL
                     AAHSPEVKFGAPSEFLNRTEGLPGLKAKYGLDVSPNNFVAISDGGGPATVRALLDGTV
                     TAADIFSTSPAIRQNNLVVLEDPKNNFLAANVVPLVSSQKKSDQLKTVLDAVNAKLTT
                     DDLIELNTVTSGNAGIDPEDAARKWVQDNGFDKPLQG"
     gene            16981..18111
                     /locus_tag="VFC01_18745"
     CDS             16981..18111
                     /locus_tag="VFC01_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN81701.1"
                     /translation="MITFDGVTKQYPDGTVAVDDITLEVPDGTLTVFVGPSGCGKTTS
                     MRMINRMIEPTSGTLTVNGDDVTKVDPVKLRLGIGYVIQSAGLMPHQRVIDNVATVPV
                     LKGESRRTARKAALGVLERVGLDPKLANRFPAQLSGGQQQRVGVARALAADPPILLMD
                     EPFSAVDPVVREELQAEILRLQGELRKTIVFVTHDIDEALKLGDRVAVFGRGGVLEQY
                     DEPSRVLSNPAGDFVAGFIGADRGYRGLQWRHSTGLPLHDIRTVGEDEIEGLTLGPTD
                     WVLIVKSDRTPFGWINAEGVDVRRKGGSLYDSTYGGGSLFRPDDSLRQALDSALSSPS
                     GLGVAVDGDGRPIGGVLADDVLAALNAQRPEALPKLGSAEEG"
     gene            18114..18782
                     /locus_tag="VFC01_18750"
     CDS             18114..18782
                     /locus_tag="VFC01_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN81702.1"
                     /translation="MRYLLTHLDDAWALTVIHLRLSLIPIVLGLLIAVPLGALIQRTT
                     TPRRLTTIIASIIFTIPSLALFVVLPLIIPTRILDEANVIVALTLYTTALLVRAVPEA
                     LDNVPEPVRDAATAVGYKPLVRMLKVELPLSIPVLVASLRVVAVTNISMVSVGSVIGV
                     GGLGTWFTDGYQSDKSDQIIAGIIAIFVLAIVIDTLIMLGGRLLTPWTRASRVARPKV
                     QAAA"
     gene            18779..19549
                     /locus_tag="VFC01_18755"
     CDS             18779..19549
                     /locus_tag="VFC01_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN81703.1"
                     /translation="MNFLQQALSYIFTAANWGGPAGLAARIAEHLQYTVVAVLVSVLI
                     AVPIGMLIGHTGRGTFLIVTGVNALRALPTLGVLLLGVLLWGLGLVPPTVALMLLGIP
                     PLLAGTYAGIANVDRAVVDAARSMGMTESRVLLRVEVPNAMPLILGGLRTATLQIVAT
                     ATVAAYASLGGLGRYLIDGIKVRQFYLALVGALMVTALALILDAALAFAVWASVPGSG
                     RLRRMPQPLLGDEVILESGSRAQTTSVARYERGILRLR"
     gene            19554..20150
                     /locus_tag="VFC01_18760"
     CDS             19554..20150
                     /locus_tag="VFC01_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HZN81704.1"
                     /translation="MSEAGGSESTSAWPATLTWRAHDVPRMESVRVQLSGNRIKAYGR
                     IVAAATPSHPAFSASYDLVTDEAGGTKRLSLTVTLAERERQLSVARDEENMWLVQHHT
                     GESKRAAYDGALDVDLIFSPFFNALPIRRTGLYQRTDSITVPVVYVRIPEMTVDPAVI
                     SYSSAPDGIKLHSPVAETTIRVDDEGFILDYPGLAERI"
     gene            complement(20113..21072)
                     /locus_tag="VFC01_18765"
     CDS             complement(20113..21072)
                     /locus_tag="VFC01_18765"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HZN81705.1"
                     /translation="MTNTPICVVGLGLIGGSVMRAAAAAGREVFGYNRSIDGVQAAKF
                     DGFDATENLDEALARAADSNALIVLAVPVPALSQMLGRIRDSAPECPLTDVTSVKHCV
                     LDEVRSFGLLERFVGGHPMTGTAHSGWSAGNARLFVGAPWVISVDDHVDADIWAMVMN
                     LALDCGSVVVPARSDEHDAAAATISHLPHLLAEALAITAGEVPLAFALAAGSFRDGTR
                     VAATAPDLVRAMCEANADQLLPVLERALDLLGNARDALAAKSPLSDLVEAGNAARTRY
                     DSFSRPEIVTIVIGAENWRQELAAAGRAGGVIRSALPTLGSRG"
     gene            21166..21687
                     /locus_tag="VFC01_18770"
     CDS             21166..21687
                     /locus_tag="VFC01_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine deaminase-associated protein"
                     /protein_id="HZN81706.1"
                     /translation="MGAQSAPAQGQAADMPDGFGVAVVREDGKWRCSAMRRAALTSLT
                     AAETELRELRSAGAVFGLLDVDDEFFVIVRPAPAGTRLLLSDATAALDYDIAAEVLEK
                     LDNDISPEELEDADPFEEGDLGLLADVGLPEAVLGVILDESDLYADEQLGRIAREMGF
                     ADELSAVLDRLGR"
     gene            21684..22142
                     /locus_tag="VFC01_18775"
     CDS             21684..22142
                     /locus_tag="VFC01_18775"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZN81707.1"
                     /translation="MKTDEDLIRVALEAAGAAGPRDVPVGAVIVAADGTELARAANMR
                     EEIGDPTAHAEILAMRAAAAVLGDGWRLEGTTMAVTVEPCTMCAGALVMARVARLVFG
                     AWEPKTGAVGSLWDVVRDRRLTHRPEVRGGVLASECAAPLEAFFAQQRLD"
     gene            22172..22262
                     /locus_tag="VFC01_18780"
     tRNA            22172..22262
                     /locus_tag="VFC01_18780"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22206..22208,aa:Ser,seq:cga)
     gene            complement(22308..22367)
                     /locus_tag="VFC01_18785"
                     /pseudo
     CDS             complement(22308..22367)
                     /locus_tag="VFC01_18785"
                     /inference="COORDINATES: protein motif:HMM:NF042831.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
BASE COUNT         3792 a         7317 c         7463 g         3810 t
ORIGIN      
        1 tggggttcag cgccgttgga ctgggtgctg gcatcgcgaa cgcggcacct cccgcgcccg
       61 tcgtcgcggg aactctgtgg cagcaagatc gagaccacgg cggtgggggc tattggggcc
      121 atggcgacgg tcgaggctgg gacgaccgtg gaggctgggg ctacggcgga aactacggct
      181 acgggccagg gtggggctgc atcacaggtc cgtttgggca catcacctgg tgcccctagt
      241 cgcggatcgt gcccgcagca gtcggcccgc cacccgctcc tctggtggcg ggccgaacca
      301 atagatcaga cgcgggatgg ccgggatccg accagacagc cttggacgcc tagcagcgga
      361 atgcgtgcac cccaggacaa tcgcggatgc atgcgaactt gcgtggacgc gctgaaagta
      421 tcagacggtt acatatcgcc ccggccatcg gccgggcggt tcgccggacc tatgcaaacc
      481 gcgcggcgag tccccgtgcg ttcatgtggg cgatcgcctc gatagagatc atcgggttga
      541 cgcccgaggc cgtcgggaag gccgagccgt cgcacaccat gaggtcgcgc acgtcccacg
      601 tctggcctgt cgggtcgcat gctgaggtcg cgggcgagcc gcccatgcgc gccgaggcca
      661 tgatgtggaa agagcccagc acgcactggc cgggcccgta gccagcggcg tcgcaatcgg
      721 ccatgaaccg cgcgcggtcg ccgtcgcgtc cgggctcgta tgcgacccag cggttttggc
      781 ttgagaagat ccgcttcgcg cccgccgcct cgtgtatctg cgcggcgccg tcaaggcctg
      841 tgcgcaggtg accgaggtcg tatttggaca actggtagcg gaccaccggc tggccgtcgc
      901 gtcctacgcg gacctcgccg ccgtctcggt cgcggaggag tacggcgatc cccgcggagt
      961 acggcagcgc ctccatcagc tcgcggtgcg cgcgggcgcc acgccatggg aggaacgaga
     1021 cggcgatgtg cggctgaatc gaggcggttt cgaacttgac gccgtagccg tcgtcgaggt
     1081 cgcgaaactg gtcggagtag agcgcctgca tcaccccttc ccatggccgt acctcgtctt
     1141 cgtacgtggc catgaccggg aacacgggat gaagtttcag atgcttgccg atgttggtgt
     1201 tgtcgagccc cgatcggcgc agcagcgccg gcgtgtgcag cgccccgcag gcagcgacga
     1261 ccgcacgcga gcggacggtg acgcgatggc cgtcgagcgt gcgggcttcg acgcctcgcg
     1321 cggcgccgtt cactacgatc acccgctcgg cgcatgtgtt tatcaccaag cgggcgccgg
     1381 cggcggccgc gtcggccagc caggtcttgg tcaccgactg tttggcgccg attcggcagc
     1441 cgaagcagca gtacccgcac tccttgcctt ggtcgcagcc gcgcacgttg cgcggcattg
     1501 cgtcgatgag ccagccgagc gcatcgcagc ccgcgcgcat gatctcgtcg cggcgtgagg
     1561 gcttgttgtg tttgaggttg acgccgagcc gctcgaccat ggcatcaagg ctgcgcgtgt
     1621 actcctcgcc ggcgaatgcc ggcacgccgt ggccggccca ttcctcgcgt acgtcgttgg
     1681 gcgtgcggaa cgacgtcgaa tagttgacga cggtcccgcc gccgaggcac gagccggcga
     1741 gcagcccgac gctttgatcg tgggtcgcct gcggtgcgcc gaggtagagg ccggtcaggg
     1801 cggtgacctc ggagccgtcg aaatcggcgt cgtcgtagta gccgcccatc tcgaggacga
     1861 cgacgtcgag gccggctgcg gccagcacgc ccgccgccgt gcccccgccg gcacccgagc
     1921 cgacgatgac gacgtcgcag agcagatccg tatcggccgc gatctccagc ggcttcagcg
     1981 gcttcggcgg ggcaccctcc ttttttccga gtggaccggg gtagccgatc gcctcccaca
     2041 ccggattggg tgaaccatcc gggttcggca gcatgtagga gacgagcagc gcgcccttgc
     2101 gcagcgcctg aaaggcggcg cggcgctggg gcagccgcga gtcacaccac gacagcagga
     2161 cgcgctcgcg ctcggcctgg cccagcgtgc tgaagcgccg caggccgccg ccgccgagag
     2221 cggtcagcag cggtgtgtcc cagaggccga gcagttgcgt gagctggcgc cgctccgcct
     2281 cgcgcgggtt cgcggcgacg gcgcctagga gtgcgtcgac cactccgagc tcactggccg
     2341 acggcgcgcc gttggcggcc gggagaaagg tgtcgcagat cgaggtcagc gctgtgcgct
     2401 ggcgcggcga gagttccatg tttccccctg aatctcttcg agcacagtca atttcggccg
     2461 aagatgcccc cggcgggatc gtgctccccg gccggcgcat acagctgcca ctgcgccgga
     2521 atgcccttca gtacgtgtgt gccgcggtcc tcgaacgtga tgccggaacc gttgacgagg
     2581 tccttcaccg tgctggagac gagcacctcc cggccccttg ccagcgcagc aatacgcgcc
     2641 ccgatgtgca cggcgatgcc gccgatgtcc tcgccgcgca cctcacactc gccggcgtgg
     2701 agaccggttc ggatatcgat ccccagctcc ggcatgcgct cagtgagcgt ggtggcgcac
     2761 cgaacggcgc gggtcggtcc gtcaaaggcc gccaagaacc cgtcgccggt gtgcttcacc
     2821 tcgcgacctt ggaatcggct gatctcttca cgcgtgatct cgtcgttacc gaggatgtcc
     2881 ctgacatcga cttgagtgag aacagcttcg aacgtcagcc gggccatctt cggactcatt
     2941 ccggcgcgtt ccagcgcacc catgcccgtg cggtacggcg cgctcggctg caggctgggc
     3001 gccgcccagt cgaccgtgtg gcccccgccc caatggtcga gtgttgggcg aatggcattc
     3061 atcaacttga gccatttcgc ccggccgggt gagccgtcgt cctcgccagt tccgcatgcg
     3121 taggtcccgt aaaggatcag cgcctggacc cgcttggggt acgtggcggc gaacagcatg
     3181 ccgatcgggc cgccctcgct gagcccgaac aacgccgcgc gttgactgtc cgcggcatcc
     3241 atcacggcgc gcaggtcgtc gacgcggctc tcgagggtgg gcacggcatc caccgggtcg
     3301 gacagtcctg tgccgagttt gtcgtagagg atcacccggg aaaacgacgc gaaatcgccg
     3361 atgaacgacg cccagccggg atggttccac agcagatcga catgggagaa ccagcccgga
     3421 accaccacca ggtccatcgg accgtcgccg accacctgat aggcgatgtc tccccccggg
     3481 cccttcgcgt accgcgtctc cggcaactgg gccatcagcc tcagtatccc atctgacgcg
     3541 ctgatcagga cgtttctcgc gattccagca acgatgtaac cgggtgcgct atctccaggc
     3601 atgacgctga atcagttcac ggcgacgcgc gcgtccggcg cggtcgttct catccgcctc
     3661 tatgtcggtc tgattttcgc cggcgaaggg gtgctgaagt ttgtccggcc cgacgcactc
     3721 ggacccggcc ggttcatcaa ggccggcatc cccggcggga cgttcttcgc ctacctggac
     3781 ggtgtcttcg agatcgcttg cggtctgctg attctcgtcg ggctgttgac ccggttggct
     3841 actctgccga tgatcgtcga catgctggga gcgctcggga tcaccaaagt gccgctgctg
     3901 tggggccatg ccccgctgta cccgaaggaa ggcggcttct gggacttctt ccacgaaggc
     3961 cgcctcgagg tggcgatgct ctgtggcagc gtcttcctgc tgatcgtcgg cgccggcgcg
     4021 tattcgttcg acgcccaact gaaccgtgac cgaacaccgg ccgtagtgac gtaagtgatc
     4081 cgcagcgagc gtgaggagcg ccgtcctcaa cccgtggcgg gtctgcagcc cgtgacgggt
     4141 gcggtgtatc cggattggga cgcggtgtat cgcgacaacg tcacctgggt ctaccggctg
     4201 atgttcggca aggtcggcaa ccagcccgac gcggaagact tgaccgcgga agtttttatg
     4261 gcggcgctgc gaccgctgcg ggtttcggcc acggtgccgg aggtccgcaa gtacttgcgg
     4321 atgactgctc ggacggtcct ggcgtcctac tggggtgatc ggatgggcag gccgatcaca
     4381 acgatcgagg acgatgtgcc ggaggtcatc gagccggagg ctgtggccag cgacgcgccc
     4441 gacaaggtgc gccggcttct gcagcggctg ccggacaact accgcagaat cctggaactg
     4501 cgcttcctgc aggccttttc ggtgcgcgag gccgccagtc aaatgggggt cagcgttgcc
     4561 aacgcgaagg tgctgcaata ccgcgcgctg cgcatggcgg ctcaatcggg tgaggaggcc
     4621 tcatcgtgag cgaccgaacc gtacgccgct tcgtggaccg cctgctgcga ggacaaccca
     4681 ctgaacgtgc tcgtccagac gacttcgagg ccgagcagat gaagacggcc atcgagttgc
     4741 gggcggcgcg gttggggagc gatgcaccgc gtgaggagtt cgtcaccgac ctgcatcgcc
     4801 ggctggcctc cgaaatggcc gaggcgcccc cgcctggttc ccggtgggcc ccccgccggc
     4861 gccaggtcgt catcggcacc tcggtcgcgg cagcctctgc ggcagcgggt ttggtggtcg
     4921 gccgcaacct gctcgctccg accgagacgc gccaggcgac gccgcagggc gagctcgaac
     4981 ccaacgccgg aacatggcga cccgtcggtg cgagcgtcga ccttccggag ggcggagccc
     5041 tcgcgttcga ccttgggtcc gtcaacggct tcgttcatcg cacggatgcc aagctgcagg
     5101 cggtgtccgg ggtctgcacg catcagggct gcaagttgtg gctggatgcg cccgagagcc
     5161 ggttgcggtg cccgtgccac tcaacgtcgt tctctctggc gggaacgacg gtcacgcatc
     5221 agttgcccgt tgcgcccccg ccgctaccga agctgcaggt acgcgaaatc aacggcgtga
     5281 tcgaggtttt tgcgccgacc aagccggcgt agcgcccatg atgcggcgcg tgacggcgcc
     5341 gctagattgg ggcggtgcga ctgctgctca tcgccgatac ccatgtcccc aagcgcgccc
     5401 gcgacctacc tagtcgtgtc tgggaggaag tggagcaggc cgacgttgtg ctgcacgcgg
     5461 gcgactgggt cgagcctcga ttgctcgacc agttggaggc caggtccaaa cgtctcgtcg
     5521 ggtgctgggg caacaacgat gcggcggagt tgcgacggcg tctgccagaa cgggccgacg
     5581 caaccctggg cgggctgcgc ttcaccgtcg tgcacgagac cggctcggcc accggccggg
     5641 aggcccggat ggcgaaggac tatcccgaca ccgacgtctt ggtcttcggc cacagccaca
     5701 ttccgtggga caccaccgcc aggaccggcc tgcgtttgct caaccccggc tcaccgaccg
     5761 accggcgacg gcagccgttc tgcaccttca tgactgcgac cgtcgacaac gccgcgctgt
     5821 ccgatgtcgt gctgcacaat ctggaacgcc atgcgtgacc gcccagcccg cgtcgccgac
     5881 gtgcacgagg tcgcggcgtc gatgccgcac gtgaaacgca tcgagggtcc caagggcaac
     5941 ccgatctacc aggtgggcgg caagtcgttc gtgttcttcc gcacgccgca gcccgacgcg
     6001 gaggatcccg agacaggcga gcgctacgcc gatgtgatca tgatctgggt ggagtccgag
     6061 agcgacaagc tgtcgctcat ccaggacccc gactcgccgt tcttcaccac cgatcgtttc
     6121 gacggccacc cgtcggttct ggtgcgcgcc aaaaggttac gtgagatcag ccgcgtcgag
     6181 ctgaccgagc tgatccagga tgcgtggctg tcgcgcgcat cgaagcgacg cgccgacgtg
     6241 tggctggccg accacggcag ctagtcagcc ggcctcggcg gcgcagatca ggccgatgtg
     6301 cttggtagcc caatcgccca gcggtccaag ggcttccagc aattcttcgc ccgccggcgt
     6361 catcgagtac tccacccgtg ggggcacctc gtggtagctc tcgcggtgca caacgccgtg
     6421 tcgctgcaac tccttgaggt gctgagtcag cattttctgc gagatgccgg tgaggctgcg
     6481 gtgcaacgca ttgaaccgct tgggaccctc cttcagctcc cacaggatca gcggcttcca
     6541 ctttccgtcc accaccgcca tcgccgcgtc gagtccgcag gtgtacttgc ccaacgtcgt
     6601 cactggtcac tcccattagt ttcggaaagg taagtatcgc acctattagt gggtacttgt
     6661 cggatagttc gcgtcacctc aacgatggtc gcatgacaac tacaaagcag acggtgagtt
     6721 tcctcggcct cggcgagatg gggtcggccc tggcacgcgc gacgatctcc gcgggccatc
     6781 ccacaaccgt ttggaaccgc accgccggca gggcgaccgc cctcgtcgag gccggagcgc
     6841 gcgcggtcag caccgcagcc gaggccgtcg aggcatccga tctcatcgtg gtgtgcctgt
     6901 tcgatcaccg gtccgtccag gaggtgctcg acccgatcgc tgatcgcctg gcaggccgac
     6961 acctggtcaa cctgaccacg acatcacccg agggcgcacg cgaacttgct gactgggcca
     7021 cgggtgtcgg cgccgattac ctcgacggag gcatcatggc gacgccggag atgatcggga
     7081 cgccgcagtc caacatcctc tacagcggtt cgcagctgct ctacgacgac caccggcatc
     7141 tcctcgagtt gtggggcgct ccagagtatt tcggcgatga cgctggtatg gcatcgctgt
     7201 acgacctggc gctgctgtcg gggatgtatg tgatgttcgc aggcttcttc cacggcgcgg
     7261 ccatggtcgg agcggcagga gtgccggcga aggagttcgc cacgcgtgcc gccgcatggc
     7321 tgcagaacgt ggtgttcgcg atcaccgagt acgccgagat catcgacggc ggtgactact
     7381 cgctgcccgg ccagcagagc ctggagttct ccgatatcag tgacatcatc gacgccagcc
     7441 gagaccaagg catcaacacc gaactggtcg acgtcgtgca gcgcctcatc catcggcaga
     7501 tcgacgcggg ccatggccgc gatgggttcg cccgcatcat cgagagcatc aagcaccacg
     7561 gggcgacggc atgacgaccg tcagtgttct cggacttggc ccgatgggcc aagcactttc
     7621 gactgcattg tttgacgcga acttggctgt cacgctgtgg aaccgcaccg agtcgagagc
     7681 cgatgcactg cgcagacgcg gtgcagcatg ggcggcaaac ccggccgaag cagttgccgc
     7741 caccgagcta accgtggtca acgtcgtcga ccacgatgcg gtcgactccg tgttggatgc
     7801 cgcgggtgac gccgtagccg accgcgtggt cgtcggactg agctccgaca ctcccgaacg
     7861 agcccggcag accgccaaac tcgtcgaaga ccgcggcggc cgctacctcg acggcgcgat
     7921 catgtcgccg accagcacaa tcggaacacc cagcgcaaga ctacttttcg caggaccgca
     7981 ggacgtcttc gaaacccatc gcgaggtgtt tgcggcgctg ggcgcatcta cttggctcgg
     8041 cgaggaccac ggccgcgccg cggccttcga catgtcgctg cttgatctgt tctggacatc
     8101 agtcagcggg ttcctgcacg cgctgacggt ggccagggcc aacggcatca cgccgagtga
     8161 attgctgcct catgcccaag gcatcgtcga tattctgccg ccgatcttcg aggaattcgc
     8221 cgagcgcatc gaagccggca gacacgacaa cagcagcgcg ccggtgtctt ccgtcgccgc
     8281 gtcggtcagg cacttgattg cggcgtcgca ggatgccggt gtggatgccg gcgcactgga
     8341 agcgttccgc ggttacgtcg acgcggcggt cgccgagggc tacggcgccg aggagtccag
     8401 ccggatcgca gcagcgatgg cccgtcgcta agcgggtggc accgtggcgt gtgaggcgcc
     8461 gcaaatgaac agggccagcc gggcgggctt gtcgctgggg ttcttccacc ggtgccgcgt
     8521 cccgttctgc acgaccgtgt cacccggatg cagggtgacc tcggcgccgt cgtcgagctc
     8581 gagcacgatc gtgccctcca agaccacctc gaagtcgatc gtgtcggtcg tatgcatgcc
     8641 gggatccgac gggtccaggt agcgaagcag gcccggtagc ttggcctcca cgtccgccaa
     8701 cgctgcctcg tctgccgcgc cgggctgttc ggcgccgggc tccaccgtga agatgccgaa
     8761 gcggaacccg ccgatcggtg ggaagtaggt acgccactcg ggcatggtgc cgtcatcagg
     8821 aaactgtggg gtcttgtccg cgccccacaa cagatacggc tggaactgcg gcatcatcgt
     8881 cgttgtgacg ggccctacca tctcgtcgct gacgaacact gacttgccgg acgcgttgtg
     8941 cccggtgaca actcggcgga cattcatgtc gtcagtcaag caccgcggcc aggaattcgt
     9001 ggcaatgtct gcgtcatgga ccagaagccg cccaccgccg tcatcgaatc cgcgaacagc
     9061 gagcacctga cgcacctgcc gttcgaggac acccaggact tcgaggacac cgaccgtggc
     9121 ttcatcggtg ccctcgaacc ctgcgtcgtg aaggccgccg acggaagagt ggtgtgggac
     9181 aacgacgttt acgccttcct cgacaccgag gtgccgacga gtgttcaccc cagcctgtgg
     9241 cggcagagcc agctgtgcgc caaacagggt ctctacgagg tggtcgaggg gatctaccag
     9301 gtccgcgggc tcgacctgtc caacatcagc ttcatcgaag gcgacaccgg ggtgatcgtc
     9361 atcgacccgc tcgtctccac cgagacggcc gccgccgcgc tgaagctgta tcgcagccat
     9421 cgtggaaatc gcgccgtcag cgctgttatc tatacccaca gccacgtcga tcatttcggt
     9481 ggcgtgctcg gcgtgaccac ccaggccgac gtcgacgcgg gccgggtggc agtgctcgcg
     9541 ccagagcatt tcacggaaca tgctgtgcag gagaacgtct atgcaggcac cgcgatggcg
     9601 cggcgtgctg cctatatgta tggcgcggcg ctggaccgcg gcccgcaggg gcaggtgggt
     9661 tgcggactcg gacaggtcgg ctcgacgggt gaggtcggcc tgatcgtgcc gaccgtcgac
     9721 atccgcgaaa ccggtgagac gcacaccatc gacggtgtcg agatcgagtt ccagatggca
     9781 cccggcaccg aggcgcccgc cgagatgcac ttctacttcc cgcgattccg cgcgttgtgc
     9841 atggccgaaa acgccaccca caatctgcac aacctgctga cgctgcgcgg tgcactggtc
     9901 cgcgatccac acgggtgggc gggttacctg accgaggcca tcgatcgctt tgccgaccgc
     9961 gccgacgtgg tgttcgcctc gcaccactgg ccgacctggg gccgcgacca gatcgtcgaa
    10021 ttcctttccc tgcaacgcga tatgtacgcc tacctgcacg accagacgct gcgacagctg
    10081 aaccagggtt tcaccggaat agagatcgcg gagaacttcc agatgccgcc ggctctgcac
    10141 aaggcgtggc acacccacgg ctactacggc tcggtcagcc acaatgtcaa ggcggtctac
    10201 cagcgctaca tgggttggtt cgacggcaac cctggccgac tctgggcgca tccgcccgaa
    10261 gccatcgggc cgcgctatgt cgacgccatc ggcggcatcg accgcgtcgt cgacttcgcg
    10321 cgcaaggctt tcgaagaggg cgacttccgt tgggcagcaa cgctgctcga ccatgcggta
    10381 ttcaccgacc agaaccacgc ggcggcacga gagctgtacg ccgacaccct tgaacagctc
    10441 gcctacggcg cagagaacgc cgtctggcgc aacttcttcc tcgcgggcgc gaccgagctc
    10501 cgcgaagcca acttcggaac tccgacggtg gcggcgtcgc cgtcaatcat cggccaactg
    10561 acacccgagc agctgttcga caccttcgcg atcaacatca acggacccag ggcttgggat
    10621 ctcgatgtcg cggtcgacat catcttcctc gacgtcgaca ccaactaccg gctgaccctg
    10681 cgcaacggtg tactggtgta ccgcaaagtg cccgccgacg catccaccgc caacgcgagg
    10741 ctcaaagtcg caaacaagct gcggctcatg gcacttgcgg cgggagacaa cgattcgcca
    10801 ggactggtga tcagcggaga cgcgggcgcg ctggcgtcga tcatcggggt gctcgaccgg
    10861 cccgacccga acttcaacat cgtcacccct tagcgatgtt gcgactccaa tagcgcggca
    10921 cgtcaagcgg gcccaacaag ccgggccgcg cttggcacac tgccgggatc gagttgagca
    10981 ccggcatcgc ggacaacgtg tcgccaccgg ggccgaagct caactcgacg gcgtagctgg
    11041 gatcgccctc gacttcgatg cggaaagcca agtccaccgg ggcgtacggc tgcttccact
    11101 gcttgccgac gtcgcgggcg acgcagtcca catgctcgac gaccgcgaac ggcttgccgc
    11161 gcgacagcgc ttgatattcg aagtgcacga cggacgctgt gcccgccgcg acggtgccga
    11221 agccggtctc gacgtcatgg tccacgctgt cggtctcata caccacgcgc gtttcctcga
    11281 cctcgacacc gagcacctcg gccagctggt acagcgtcgg cgcccacatc gtctcgatga
    11341 aaccgccggt caccagcagc ggctgaaacc cgggcggctt gccgaagccg aagtactccc
    11401 ggcagacgaa ctccgcggtg tagtcacccc accagcccag ctccatgacc cggatacagt
    11461 cgatgcggtt cgcgacggct aacgccgtga tcgccagatc ggttgtcgcc caaccgggat
    11521 cgatgccggt gaagaagcag ctgctgtttc cggcgcgaca cgcggcctcg ataggcatgc
    11581 gaaactcggg tggcgtggtg gcggggtgcg cgatcgcgaa gccggaaaac gtaacgacgt
    11641 tggtgccccg ctccaggaac gggacgaggt cggagatcgc gtcggcttcg cggccgatgc
    11701 tgttgccgaa gtaggtaagg cagtcggcgc cgaggtcgac tagctcggac cagtcgttgg
    11761 tggtggtgac gccgacggcg tccaggccgc cgagctcgcc cgagtcgcga ccggccttct
    11821 cgggcgtgct gacgtaatga cccacgagct tcagctcggg atcgttgatg atcgccttca
    11881 gcgcggtcgc gccgatgttg ccggtgccgc aatgcaccac gctgtaaccc actggcttac
    11941 tgtaacaccg acagtagcgg cacaccgagt ggccacctaa tgcacgcttc ggcgcgattc
    12001 gcgtgtcata actggccact cggggaacta cgggattccg ccgtcgacgc ggatgatcga
    12061 tccgctggtg tagctcgacg cgtctgacgc cagatacagg acggcgccga tgatctcggg
    12121 tggattgccg agccgcttga gtgcgaagtt ctgcgcgccc ttcgtcgtct tcgggatatc
    12181 ccaggccttg ctgatgtcgg tcaggaaggg cccgggcatc aacgtgttga ctcggacggt
    12241 cggcccgaag gccagtgcga gcccctcggt caacgcgttg agcccggcct tggcggccga
    12301 gtacggcagc tgctccgggc ggggacgtcg tgacccgctc gagctgacgt tgatgatcga
    12361 accgccgccg tcggccacca tccgctcggc cacgagtgtc gacaagcgga acgggccttt
    12421 gaggttgagg ttgaccacgg cgtcgaacag tttctcggtg accgaactca acgattcgta
    12481 gagcggcgac atgccggcgt tgttgataag gacgtcgacc ttgccgaacc gctcgtaggc
    12541 ggcgtccact agtccgtcga gctcatccca ccgcccgacg tgcacctggt agggcaatgc
    12601 agcgcgaccc gtcgcggctt cgatctcctt ggcagtctcc acgcaggtct cgtacttgcg
    12661 gctggcgatg accacgtcgg ccccgcagct ggcggttgcg aacgccatct cgcggcccag
    12721 gccgcggctg ccgccggtga tcagcaccac ccggtcagtg agatcgaaca gttcgtcggc
    12781 atagcccatc tgtgtgctcc ttattggtcg cggaccgtct cgcgaatcac tcggtggatg
    12841 aacccgagct tggcaactac cggttcgggg atctcgaacg ggtagagggg attcttgccc
    12901 atcgcagtgt tcacccggtt gaagaacaac gagatccatc gccagtcgaa cagcagccgc
    12961 tgtatcggca cgtcgtcata ggactccagc ggaacgatgt cccgcggcgc cgagaaccgg
    13021 acgcggtcgg cctgtaacac catgcccgcc tcgcggctgg tgtcgatggt gtcggcgatg
    13081 tggaggtagt gggcgaagca ctcggcgaag tcctcccatg gatgcatggt ggcgtactcc
    13141 gaaatgaacg tcgcgtccca gccgtcaggc gcaccgaact tgtagtgtcg cgcgatggca
    13201 tcgctgtagc tggcgcgctc atcgccaaac agcctgcggc actcgtcgag gtatttgctt
    13261 gcgccagaac cactctcgac cagaacgtgc tggtagtagt ggccgacttc gtggcggaag
    13321 tggccgagca tcgtccggta cggctcgccg agccgtaccc gcaacgactc gcggtagtcg
    13381 tccagggatt cgacgaggtc gatggtgatc acgcctcctg cgtgtccgat cgtcaccttc
    13441 tcgccggtgc tgtagctgga tacgagatcg aacgccaggc cgccatcgtg gtgccagaac
    13501 gggtcgatgg gcagcccgat gtcgccgagt tggtaaacga ggcggcgtaa cgacctcgca
    13561 gtgggtacaa gcttctcgcg cgccaacgtg tcatcggcgt cgggctcgcg ccgtatcagc
    13621 gagtcggcca ggcaccggcc ccgttcgtcg ggcgactgtt cctcgggggc cagccagttg
    13681 cagcccagcg tctcactccg tgtgcagcga acccagcgct ggccgccaac ttgggccaca
    13741 tcgtcggcaa ccggctgcat cgacctcgtc ggcaggtgaa agccgagcgt ggtgtggcag
    13801 gtggcgcact ggtccgactc gaacgcagcg aaggcattgc acactgggca ggcgaatgcg
    13861 cgcacgggcc ctatggtgac acggtgcaac tcctactcat caggcacgcg cttccgctgc
    13921 gcagcgagcc gggtctgggc tcggaccccg acctgtccga ggagggcatc gagcaggcca
    13981 agcggctgcc cgatgcgctg gcgcgcttcc cgatcacgcg gctggtcagc agcccgcagc
    14041 gccggtcgat ccagacggcg cagccggttg ccgacgcact cgggctgacc gccgagatcg
    14101 atgaccggct cgcggagtac gaccgcgaca tggagcacta catccccatc gagcagatcg
    14161 ccgccgagtt tcccgaggag ctggcgcgac tggcccaggg gcatctgccc agcactgtcg
    14221 acgagaccgc gttcctggat cggatcaacg cggggatcgc cgacctggtt gcctcgggcg
    14281 accacgagga caccatcgcg gtcttcaccc acggcggcgt gatcaacggg ctgctgcaca
    14341 acattcttgc caccgagaag atcctgtgcg tcaacgtcga ctacgccggc gtcacccgac
    14401 tgctttcctc acgcgagggc aacctgtatg tggccgccgt caacggcacc gagcacgtat
    14461 gggatctgct gccgcgaaat cagcggtggt aggaaagtcc ggtgactact tcgctcgaag
    14521 gcctggacct cgacgccctg gaccgccatc tgcagtccgt cggcatcgcg cgtagcggcg
    14581 aactgcgcgc cgagctgatc tccggcggcc ggtccaacct gacgttcctg gttttcgacg
    14641 acgcatccaa gtgggtgttg cgcaggccgc cgctgcatgg cctgacgccg tcggcccacg
    14701 acatggcccg cgagtacaag gtggtggccg cgctggccga caccccggtg ccggtggcac
    14761 gcgccatcac gatgcgcaac gacgactcgg tgctgggtgc gccgttccag atggtcgaac
    14821 acgtgccggg gcgggtcgtg cgccatgacg acgagctcga gaagctcggc gacaacgccg
    14881 tcatcagcga ctgcgtcgac gcgctgattc gggtgctcgc cgatctgcac gccgtcgacc
    14941 cggaggcggt cgggctcggt gatttcggaa agcccaccgg ctatctcgaa cgccaggtgc
    15001 gacgttgggg cgggcaatgg gaactcgttc ggcgcgaaga ggatccttgc gacgcggacg
    15061 tcaagcggct gcattcggct ctggcagaag cgattccgcc gcagagccgc agctcgatcg
    15121 tgcacggcga ctaccgcatc gacaacacga ttctggatgc gcaggacgcc accaaggtgg
    15181 tggccgtatt ggactgggag atgtccacat tgggcgatcc gctgagcgac gccgcgttga
    15241 tgtgcgtgta ccgccatccg atgttcaaca tcatccacgc gtcctcggcc tgggcgtcgc
    15301 cgctgatgcc ggaggtcgac gaactcgcga accggtattc gcagtcgtcg ggacagtcgc
    15361 tggataactg ggacttctat atggccctgg gctacttcaa ggtggcgatc atcgcggcgg
    15421 gcatcgcatt tcgggcccgc atgggcggtg gtgtcgccaa ggacaccgat caggtggaca
    15481 aggcggtggg cccgctgatc gcggagggcc tcaacgtaat cggcagaggt tagaagccgg
    15541 ccttcaagtt cttcttcgcc gcccgacgca gttgcacgat gcgcgccgaa cccgccgcga
    15601 ccgtcagcag accgccgcac accgcggcca gaagaatccc cacacccacg ggcaggttcc
    15661 actcccatgc gaagaagtgg atggtcgccg aatccgtgtt ctgcaggatg aaaacgagca
    15721 gcaggatcag gatcacgaat ccgacgatga gcgccgacca cagggccgcc gctcgggtga
    15781 acttgaccgc cgactcgggc gaaacgaagt attcgccggg tgtggcggtg ggttcgggtg
    15841 gtgtccccct cgacgattcc ggcagtgcgg cggacgacgg ctgctgttca gccggatcgc
    15901 tggtcatgac tccatctttg ccccttggtg cgtcgaatga aaccaccggg gcgctcacgg
    15961 tgagccaaag cgtggcatct ggtctcacat gccgctaggg tccgggggag ctagcgcgcc
    16021 gagaggacat tggcatgtcg agcacgcgta ggtcgagtct ggcaatcctg gcatctcttc
    16081 tcgccctgat agccacagcg tgcggcagct cgaacccact tggcggtggc acggtatccg
    16141 gtgacctcaa gtcgatcgtc gtggggtccg cggacttccc ggagtcgaag atcatcgccg
    16201 agatctgggc gcaggcgttg gaggcaaacg gcttcaccgt cggcacgcag tacggcatcg
    16261 gcagccgcga aacctacatt cccgcggtcc gggaccactc catcgacctg attcccgaat
    16321 acaccggcaa cctgctgcag tacttcgaca agaacaccaa ggtgaccacg ccggaggcag
    16381 ttgaactggc gctgctgcgc gttttgcccg gcgatctgtc gatcctgacg ccgtcgccgg
    16441 cggaggacac cgacaccgtc acggtcagtc agccgaccgc gcagaaatgg aaactcaagt
    16501 ccatcgccga tctggcggcg cattcacccg aggtgaaatt cggggcgccg tcggagttcc
    16561 tgaaccgcac cgaaggcctt ccgggactca aggccaagta cggcctcgat gtctcgccca
    16621 acaacttcgt cgcgatcagc gacggaggag gacccgcgac cgtacgcgca ctgctcgacg
    16681 gaaccgtcac ggcggccgac atattcagca cctcaccggc gatccggcag aacaacttgg
    16741 tcgtcctcga ggacccgaag aacaattttc tcgccgccaa tgtcgttccg ctggtgagtt
    16801 ctcagaagaa gtccgatcag ctcaagacgg tgcttgacgc cgtcaacgcc aagctgacca
    16861 ccgacgacct catcgaactc aacaccgtga catccgggaa cgcgggcatc gacccggaag
    16921 acgctgcacg aaaatgggtc caagacaacg ggtttgacaa acccctccag ggatgagcgc
    16981 atgatcacct tcgacggcgt caccaagcaa taccccgacg gcacggtcgc cgtcgacgac
    17041 atcaccctcg aggtgcccga cgggacgttg acggtcttcg tcgggccgtc cggctgcggc
    17101 aagacaacgt cgatgcggat gatcaaccgg atgatcgagc cgacgtcggg aaccctgacg
    17161 gtcaacggcg acgacgtcac caaggtcgat ccggtcaagc tgcgcttggg aatcggctac
    17221 gtgatccaaa gcgcagggct gatgccgcat cagcgggtca ttgacaacgt cgcgacggtg
    17281 ccggtgctga aaggcgaatc gcggcggaca gctcggaagg cggcgctcgg cgtgctggaa
    17341 cgcgtcggcc tcgaccctaa actggccaat cgcttcccgg ctcagttgtc cggcggtcag
    17401 cagcagcgcg tcggcgtggc tcgcgccttg gctgccgatc cgccaatctt gttgatggac
    17461 gagccgttca gcgccgtaga ccccgtcgtg cgtgaagagc tgcaagccga gatcctgcgg
    17521 ctacaaggtg aattgcgaaa gacgatcgtg ttcgtcaccc acgacatcga cgaggcactc
    17581 aagctcggcg acagggtcgc ggtgttcggc cgcggcggcg tgctggagca gtatgacgaa
    17641 ccgtctcgtg tgctgtcgaa cccggcaggc gatttcgtcg cgggtttcat cggcgccgac
    17701 cgcggctatc gcgggttgca gtggcggcac tcgaccggcc ttccgctgca cgacattcga
    17761 accgtcggcg aagacgagat cgagggtctc acattgggcc caaccgactg ggtgttgatc
    17821 gtcaagtccg atcgaacgcc gttcggctgg atcaacgccg agggtgtgga cgtgcgccgc
    17881 aaaggcggtt ccctctacga cagcacgtat ggcggcggat cgctgttccg tcccgacgac
    17941 tcgctgcggc aggccctcga ttcggcgttg tcgtcgccgt ccggtctggg cgtggcggtc
    18001 gacggcgatg gccgcccgat cggcggggtg ctggccgacg atgtgttggc cgcgctgaat
    18061 gcgcagcggc ctgaagcgtt gccgaaactc ggctcagccg aggaaggttg agcgtgcgct
    18121 atctgctcac ccatctcgac gacgcctggg cgttgacggt gattcatctt cgactgtcgc
    18181 tgattccgat cgtgcttggc ctgctgatcg cggtcccgct gggggcgctg atccagcgca
    18241 ccaccactcc tcgccgattg acgacaatca tcgccagcat catcttcacg atcccgtcgc
    18301 tggcgttgtt cgtcgtgctg ccgctgatca tcccgacccg catcctcgac gaggcgaatg
    18361 tcattgtcgc gctgacgctg tacacgacgg cgcttttggt gcgagcggtg cccgaggcgc
    18421 tcgacaatgt gcccgagccg gtgcgcgacg cggccaccgc cgtcggctac aagccactcg
    18481 tccggatgct caaagtcgaa ctaccactgt cgattccggt gctggtggcc agcctgcgcg
    18541 tcgtcgccgt caccaacatc tcgatggtgt cggtcggctc ggtgatcggt gtcggcgggc
    18601 tgggcacgtg gtttaccgac gggtatcagt ccgacaagag tgaccagatc atcgcgggca
    18661 tcatcgcgat cttcgtgctc gcgatcgtga tcgacacgct gatcatgctc ggcggccggt
    18721 tgctcacgcc gtggacacgc gcgagtcgcg tcgcccgacc gaaagtgcag gcggcggcat
    18781 gaacttcctg cagcaagcgt tgtcgtacat cttcactgcg gccaactggg gtgggcccgc
    18841 cgggctggcc gcccggatcg ccgaacacct gcagtacacc gtcgtcgcgg tgttggtgtc
    18901 ggtcttgatc gcggtgccga tcgggatgct catcgggcat accggccgtg gcacgttcct
    18961 catcgtcaca ggggtcaacg cgcttcgcgc actgcccacg ctgggtgtgc tgttgctggg
    19021 tgtgcttttg tggggcctag gtttggtgcc gccgacggtt gcgctgatgt tgctgggcat
    19081 tccgccactg ttggccggaa cctacgcggg gatcgcgaat gtcgaccgtg cggtcgtcga
    19141 cgccgcccga tcgatgggaa tgacggagtc gcgggtgctg ctgcgcgtcg aggtgccgaa
    19201 cgcgatgccg ctgatcctcg gcgggctgcg cacggcgacg ctgcagatcg tcgcgaccgc
    19261 gaccgtcgcg gcgtacgcga gcctcggcgg cctcggtcgc tatctgatcg acggcatcaa
    19321 ggtgcggcag ttctacctcg cgttggtcgg tgcgctgatg gtgaccgcgc tggcgttgat
    19381 cctcgacgcc gcgctggctt tcgcagtgtg ggcctcggtg cccggctctg gccgactgcg
    19441 tcggatgccg cagccgttgc tcggcgatga ggtcatcctc gagtccggat cgcgcgcaca
    19501 aacgacttcg gtagcacgct acgaacgagg gatccttcgc ctacggtaga ggggtgagtg
    19561 aagcaggcgg ctctgaaagc acatctgcct ggccggcgac cctgacctgg cgtgcgcatg
    19621 acgttccgcg gatggaatcc gtacgggttc agctgtcggg caaccgcatc aaggcctacg
    19681 gccgaatcgt cgcggccgcg acgccgtcgc acccggcgtt ctccgcgtcc tacgacttgg
    19741 tgaccgacga ggctggcggg accaagcggc tgtcgttgac cgtcacactt gccgagcgcg
    19801 agcgtcagct gtccgtcgcg cgtgacgaag agaacatgtg gctggttcag caccacaccg
    19861 gcgagtcgaa acgcgcggcg tatgacggcg cgctcgatgt cgatctgatc ttcagcccgt
    19921 tcttcaacgc gctgccgatc cggcgtaccg ggctctatca gcggaccgat tcgatcaccg
    19981 tgccggtggt ctacgtgcgg atacccgaga tgacggtgga cccggcggtg atcagctaca
    20041 gcagcgcgcc cgacgggatc aagctgcatt cgccggtcgc cgaaacgacg atcagggtcg
    20101 acgacgaggg tttcatcctc gactacccag ggttggcaga gcggatctga tcacgccgcc
    20161 cgcacgcccc gcggcggcga gttcctggcg ccagttctcc gccccgatga cgatggtgac
    20221 gatctcgggt ctgctgaagc tgtcgtagcg ggtacgggcg gcgttaccgg cctcgaccag
    20281 gtcggacagc gggctcttcg cggccagcgc gtcgcgcgca ttgcccagta ggtcgagggc
    20341 gcgctccagt acgggcagca gctggtccgc attggcctcg cacatggctc gcaccaagtc
    20401 aggcgcggtg gccgccaccc gggtgccgtc gcggaacgat cccgcggcga gcgcgaaggc
    20461 cagcggaacc tcgccggcgg tgatcgccag cgcctcggcg agcaggtggg gcagatgcga
    20521 gatggtggcg gcggccgcgt cgtgctcgtc ggaacgagcc gggacgacga cggagccgca
    20581 gtccagcgcg aggttcatca ccatcgccca gatgtcggcg tcgacgtggt cgtcgacgct
    20641 gatcacccag ggcgcaccga cgaagagccg tgcattgccg gccgaccagc ccgagtgggc
    20701 cgtcccggtc atcggatgac ctccgacgaa gcgctccagt aagccaaagg accgaacttc
    20761 gtccagtacg caatgcttca cgctggtcac gtcggtcagc gggcactccg gcgcggagtc
    20821 gcggattcgg cccagcatct gcgacagggc gggcacaggc acggcgagca cgatcaacgc
    20881 gttgctgtct gcggcgcgcg ccagcgcctc gtcgaggttc tcggtggcgt cgaacccgtc
    20941 gaacttcgcc gcctggacgc cgtcgatgga gcggttgtat ccgaaaacct cgcgtccggc
    21001 cgccgcggcg gcgcgcatta cggagccccc gattagtccc agacccacca cacaaatggg
    21061 tgtgtttgtc acccttccag gttggcacat cgccggcatc gcccgacgtc aggcttggtc
    21121 tcgattgctg gtcaagcctc ggcgtcgcga ctaccgttag cccacatggg agcacagagt
    21181 gcaccggcgc agggccaggc cgccgacatg cccgacggct tcggcgtcgc cgtcgtgcgc
    21241 gaggacggca aatggcgttg ctcggcgatg cgccgggcgg ctctgaccag cttgaccgcc
    21301 gctgagaccg aactgcgcga attgcgtagt gcgggcgcag ttttcgggtt gcttgatgtt
    21361 gacgatgagt tctttgtgat cgtgcgccct gcgcccgcgg gtacgcggct gctgctctcg
    21421 gacgcgactg cggcgttgga ctacgacatc gccgccgaag tgctggaaaa gctcgacaac
    21481 gacatctctc cggaggagct cgaagacgcc gacccgttcg aggagggcga tctcgggctg
    21541 ctcgctgacg tcggcctgcc ggaagcggtg ctaggcgtca ttctcgacga gtccgacctg
    21601 tacgccgacg agcagctggg ccggatcgcg cgggagatgg gattcgccga cgagctgtcg
    21661 gccgtgctcg accgcctggg tcggtgaaaa ccgacgaaga cctgatcagg gttgcgttag
    21721 aggccgctgg tgccgctgga ccgcgggacg tgccggtcgg cgcggtgatc gtcgccgcgg
    21781 acgggaccga actggcgcgg gcggccaaca tgcgcgagga gatcggtgat ccgaccgcgc
    21841 atgccgagat cctggcgatg cgggccgcgg cggccgtttt gggtgacgga tggcggctgg
    21901 aaggcaccac gatggcggtg acggtggagc cgtgcacgat gtgtgccggc gcgctggtga
    21961 tggcgcgggt ggcgcggctg gtgttcggcg cgtgggaacc gaagacgggg gcggtcgggt
    22021 cgctgtggga cgtggtgcgc gaccggcggc tcacgcaccg gccggaagtt cgtggaggag
    22081 tgctggcgtc cgagtgcgcg gcgccgctgg aggcgttctt cgcgcaacag cgattggatt
    22141 agcgccacgg tgggccggta agctgccacg cggtggcgtg tccgagcggc ctaaggagca
    22201 cgcctcgaaa gcgtgtgacg ggtaaccccc gtccgagggt tcaaatccct ccgccaccgc
    22261 caaagccaaa cacctgttac cgcaggtaga aggccgcccg tgctgtggtg gggctcggcc
    22321 accggcttgg attcggcaga cccacgctgc cgaagaaaaa tcgacaagcc gatgatcgct
    22381 gc
//
LOCUS       DASRJA010000429        27328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2671885,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000429 DASRJA010000000
VERSION     DASRJA010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 27328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27328
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2671885"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..489
                     /locus_tag="VFC01_18790"
     CDS             322..489
                     /locus_tag="VFC01_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81708.1"
                     /translation="MVKSYETLVDSDTRVDVNTGIVAASRLQAVIEPRDYSIDIAEMV
                     ARIPRRQARCA"
     gene            584..1417
                     /locus_tag="VFC01_18795"
     CDS             584..1417
                     /locus_tag="VFC01_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81709.1"
                     /translation="MNVILFVFAGRKQNMELQLPLVRRIITEHPNVQYHVWNLSRNDD
                     DNLYLRAIEGDRINVLNDYFGIAPWLGFNAVYRHYAHNPKYRDHLFVKVDDDVVFIET
                     GRFGEFVEAVNGNRASVVTAKVVNNGACTPTEPGLWSGFEALSLPLLDVHLSGEYAVM
                     SHTYMFKHWREMVGQPVKLIKTRDWLSINTIGYDWHMVRKIARRIGTPSPGDIAGRSF
                     TPRIKIGDEGMVNMLPRLIFQGFLTAHLSFGPQHAHLTDAQLSLWRKRYAAIGQQYLD
                     A"
     gene            1414..2112
                     /locus_tag="VFC01_18800"
     CDS             1414..2112
                     /locus_tag="VFC01_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005110292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN81710.1"
                     /translation="MKVAIVIPFRDRGNDPLRPANLRCVIEHWDGCDVPVWLSDDGRV
                     GDAQFNRSQAYNRGAWFVDADVVVFAEADMLVPYSQIRQAVAMAAAAPGLVVPFIQYR
                     YLTREDSGLVRAHDIEPGDCIPESIMDNGESIGAINVVSRETLDAIGQWDEVFEGAWY
                     DDVAMKIAFEVCAGPTRWVGGPAWHLWHKPGLGGSQLTAEDKAATARNKERLELYRQA
                     TTPERIRELTAGRL"
     gene            complement(2053..2301)
                     /locus_tag="VFC01_18805"
     CDS             complement(2053..2301)
                     /locus_tag="VFC01_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81711.1"
                     /translation="MRLNRAAVDHRRFWDAMDRLGEGQLREIETRLARRMVTEFGLDP
                     TGLALDMTYFATFIDTPAKLEPTRSQLADTLGGGGLSI"
     gene            complement(2447..4366)
                     /locus_tag="VFC01_18810"
     CDS             complement(2447..4366)
                     /locus_tag="VFC01_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HZN81712.1"
                     /translation="MNDLFTFSIDWGRALFDSAIWIARAWAIAAVCTLVVLVLIARYT
                     TWGRQFWRITGAYFTGPHSVKVWLWLAALLLSVIVGVRLNVLFSYQGNDMMSAAQAAF
                     TGIGAGDEAVKESGKHGFYTSLGIFSILATVHIARVMLDLFMMQRFMLAWRVWLTDRL
                     TGDWLTGQAYYRGRFIDERIDNPDQRIQSDIDIFTAGVGPLPNTPNNTTGNTLLFGAI
                     DAVVSVISFTAILWRLSGNLTLFGVTMPRAMFWIGFAYVLLASIVAFRIGRPIIWLSF
                     NNEKYNAAFRYALVRLRDAAESVAFYRGEAAERLQLHRRFQPVVSNYKRYINRMIRFF
                     GWNFSISQLIVLPPWLIQAPRLFAGQIKFGDVTQTATAFSNIQNGLSFFRNAYDSYAG
                     WRASIIRLHGLVTANEEGRALPSLTVTPSRDCLVELDDVEVRTPAGEQLIRPLDLRLN
                     LGDTLIITGKSGAGKTTLLRSLAQLWPYTTGTLRCPGGANETMFLSQMPYVPLGDLRA
                     VVSYPKEPGEIPDEELHRVLTKVALPQCSQRLSEVADWANVLSPGEQQRIAFARILLT
                     KPKVAFLDEATSALDEGLEFMLYDMVRTELPDTVLVSVTHRSTVGQHHEGHLELLGDG
                     EWRLSRVEGGELARV"
     gene            4859..5845
                     /locus_tag="VFC01_18815"
     CDS             4859..5845
                     /locus_tag="VFC01_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HZN81713.1"
                     /translation="MRVPTEARAFWILEPGRGEIRTVALPEPGPGEVLVRTLRSGLSR
                     GTEGLVFRGGVPQSQYDVMRAPFQEGDFPGPVKYGYLNVGVIERGPDELQGCTVFCLF
                     PHQTAYVVPADAVAVVPDEVPPERAVLAGTVETAVNALWDAAPLVGDRITVVGAGMVG
                     CCVARLVGGVPGTQVTLVDVDDTRAHIAASLGVGFALPEHALGGQDLVFHASATSAGL
                     QLSLALLAADGMVVDLSWYGDQPVQLSLGGAFHSRRLGIRASQVGDVAVARRGRRTTG
                     ERRALALDLLQDPAFDALLTGASTFDELPDVMARLSDGRLRAICHTLTYDEG"
     gene            5851..6249
                     /locus_tag="VFC01_18820"
     CDS             5851..6249
                     /locus_tag="VFC01_18820"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HZN81714.1"
                     /translation="MFSVTVRDHVMIAHSLRGEVFGPAQRLHGATFIVDATFSRVELD
                     SDDIVVDIGGATEVLRDVLADLNYRNLDEVQELAGRNTTTEVLAKLIADRLAGRVEGG
                     GLGDSASGLARIAVTLRESHIAWASYERSL"
     gene            6246..7280
                     /locus_tag="VFC01_18825"
     CDS             6246..7280
                     /locus_tag="VFC01_18825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN81715.1"
                     /translation="MSEVHVVVPDGIDDPRRPSGGNVYDRRACRGLSALGWSVHEHTS
                     PGRWPRPDGQARAGVAAALARMPSGALVLVDGLVASVVPEVLEPEAGRLRLVVLLHMP
                     QDEPREVAALSAASAIVATSQWTRQWLSDNYSLPSTPVYVVPPGVDAAKVAAGTPSGG
                     RLLSVGAVTPVKGHDVLLDALATVDDPAWRCVCVGALDLDQEFVARLRWLAEDSGLDD
                     RVCFTGPLTHDELDRAYAEADVLVVASRSESYGMVVTEALARGLPVIAGEVGGVPEAL
                     GHAADGSRPGLLVPPGDPQTLGQTLSRWLGDPRERERLRAAARSRRQRLTDWAQTSRE
                     LSAVLFEVAR"
     gene            complement(7506..8330)
                     /locus_tag="VFC01_18830"
     CDS             complement(7506..8330)
                     /locus_tag="VFC01_18830"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN81716.1"
                     /translation="MTTEPVRASSEWLRLREPADAAARASELVEKVRSYLPTHGGATI
                     HDLGCGTGSMAHWLAVRLPGPQHWVMYDRDDELLTLAAADPPDRASDGTPVTMEARRR
                     DITRMELAELAGAALITASALLDTMTADELERLVATCAGADCPVLIALSVTGRVEITP
                     AEPFDQSVRDAFNAHQRRTTSVGKLLGPDAVGAAVDSFTRLGREVLVRPSPWRLGPGH
                     AALAAAWFTGWLAGACEQRPELRAHAPSYARRRLAEAAAGHLYLTVHHQDLLVVPR"
     gene            complement(8402..8554)
                     /locus_tag="VFC01_18835"
     CDS             complement(8402..8554)
                     /locus_tag="VFC01_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81717.1"
                     /translation="MWEDELLERIASTSATNIEAGLRDRVRELRDRGMSWARIGIALG
                     MRRQSA"
     gene            9071..9448
                     /locus_tag="VFC01_18840"
     CDS             9071..9448
                     /locus_tag="VFC01_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81718.1"
                     /translation="MAVAIRLAISASLAVSGVIHAYLYVNGYRDIPIVGPGFLGQAIV
                     FCVLALLILAGGPDWLRWVAGVLSAGALVAFALSRTVGLFGFAERGWNPSPYAAVSVI
                     AEVLNAVFVAASVLSSRTKTPLP"
     gene            complement(9645..9803)
                     /locus_tag="VFC01_18845"
     CDS             complement(9645..9803)
                     /locus_tag="VFC01_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6131 family protein"
                     /protein_id="HZN81719.1"
                     /translation="MIVLGVILILVGYLLPVPGIIATIGWILLVIGLVLLVLGRMGRP
                     VGGRRYWY"
     gene            complement(10661..12982)
                     /locus_tag="VFC01_18850"
     CDS             complement(10661..12982)
                     /locus_tag="VFC01_18850"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005516012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZN81720.1"
                     /translation="MAGQFNGKIELDIRDSTPDWNAFLDPKAPGNAPNVLVILYDDTG
                     CAAWSAYGGRISMPTLRRLAANGLTYSQWHTTSVCSPTRSTMLTGRNHHQNGFGTIAE
                     SAAGFPGYSGHIPPENATIATVLRDAGWSTFWVGKNHNVPVDAFDVGANRKHWPLGLG
                     YDRFYGFIGGETNQWYPTLIEDNHFIEQPYSPEDGYHFSKDIADKAIGFIRDTKQSRP
                     DKPWYMWYCPGANHAPHHAPKEYIEKYKGQFDDGYEAYREWVLARMIEKGVLPPNTEL
                     TELNPMTPGTFIEGDSVRPWASLSEDEKTLFSRMAEVYAAYSEYTDVQIGRIIDYLEE
                     SGQLENTLIFYCADNGASGEGSPNGSVNENRFFNGYPDDTESNLAMIDKLGSAETYNH
                     YPTGWAVAFSTPYRMFKRYSQYAGGTADPMVIHWPKGFKARGEIRDQYHHCTDVVPTI
                     LECCGLTMPDAVDGVRQAPIAGVSMRYSFDDAEVPTRKETQYFEMLSTRGLWHNGWKV
                     STEHGPMINKGAFDDDRWQLFHTDVDRSEAHDLAAEHPEKVKQLSEMWLAEAKSNNAL
                     PLNDYGVEGIHALEYKVTPPEDGRYTYYPDTSEVPEASAARTLGASFKILAEVEFTKA
                     TQGVIVSQGSRFGGYTLFVKDGQLNLVYNFLGIAPEQKLSCPAPVSGKHVVGVDFAKS
                     SISEKLEVLGTMTLYIDDKAVAKADFRTQSGHYALCGEGVAVGRDSADPVSTEYSPGF
                     SFTGGRISKVTYDVGDDAYVDLERRLGAILARD"
     gene            complement(13005..13175)
                     /locus_tag="VFC01_18855"
     CDS             complement(13005..13175)
                     /locus_tag="VFC01_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81721.1"
                     /translation="MTRATPFTPSHPDLANAAGVPARLANEKYVESLARIYVPTQSLL
                     DGTYKSPNVQRT"
     gene            13846..15183
                     /locus_tag="VFC01_18860"
     CDS             13846..15183
                     /locus_tag="VFC01_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN81722.1"
                     /translation="MNVGGGAQNIHYAKSGDLNIAFSVTGEGPDLVVAPGFISHLDIM
                     WEEPSVVHFYSRLASFRRVVTFDKRGTGLSDPAMHAPTLEESVDDLRAVMDAAGCERA
                     DVLGISEGGTMAILMTASHPDRVNALVLYGTFTRLLRARDYPLGVTDEQLSALVELSA
                     KGWGEGVGLGGWAPSRRGDADLRRWWARLQRVAASPGMVRNIFALYPQLDIREVLPAI
                     QVPTLVLHRRDDRMVSIEMGRYLADRIPGAKFVELEGADHLFFTDDADTLLDEIEEFL
                     TGVRPLPAVERVLATVLFTDIVDSTKRAVELGDERWKELLGRHDAQVRRQLERFHGRE
                     VNTTGDGFLAWFDGPARAIRCATAIRDVLRGLGLEVRAGVHTGEVELRDDDISGIAVH
                     VAARVAAAAGAGEVLVSRIVVDLVAGSGLSFTARGEHPFKGVPGEWALFAVEG"
     gene            complement(15374..16333)
                     /locus_tag="VFC01_18865"
     CDS             complement(15374..16333)
                     /locus_tag="VFC01_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008261915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZN81723.1"
                     /translation="MSGWTRGSLFAALKTAGAASILGWVLVLSAGPALADPDPNPAPG
                     EPGVVAPPVPGLPPGPDPLAVPVVAGPAGGQDPTPFTGTAPFGPPRIAPANGSTVGVA
                     QPIIINFPGLVDDAGAAENAVRVSSVPPVPGKFYWMTPTQLRWRPLNFWPAHTAVTVD
                     AGGTVSSFQTGDTLVATADDATHQLTVTRNGAVEKTIPMSMGMTAGGHQTPNGTYYVQ
                     EKMPSVVMDSSTYGVPVNSTYGYRTTVELAVRFDDSGDFVHSAPWSVDDQGKRDVSHG
                     CINISPANAKWFFDNFNAGDPIVVQNSSGGTYTGNDGSNDWQS"
     gene            17064..17528
                     /locus_tag="VFC01_18870"
     CDS             17064..17528
                     /locus_tag="VFC01_18870"
                     /inference="COORDINATES: protein motif:HMM:NF018098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allene oxide cyclase family protein"
                     /protein_id="HZN81724.1"
                     /translation="MPKSGTLLSFGLLAVVGIAAGDLMAAGRATAAPQELIFTEHAAT
                     DTVTDTGASGDTAGDVLTFANEVFDAEDRNRVGSDQGMCVRTVPGKAWECFWTLSLAS
                     GQITVDGPFYDSGDSVLAITGGTGAFGNAQGDMLLTPIGTEGNAYRFTYRLL"
     gene            17591..19441
                     /locus_tag="VFC01_18875"
     CDS             17591..19441
                     /locus_tag="VFC01_18875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HZN81725.1"
                     /translation="MSPTSTSTLTARLARWIPAVGWLASYRLSWLRVDLAAGITVAAY
                     LLPAGLGYASLADLPPQAGLYACLFGGLVFWLFCSSRHTTISVTSAISLLIGSTLGTI
                     SGGDSSRFTALAAGTAILVGLIAIIAWLVKAGSIINFISESVMIGFKWGVALFLVSTQ
                     LPKLFGTPAAHGDFWENSGTFLKHLNETNLAALTIGGTALVVLVLGKLLVKKLPMSLL
                     VVVAGIVASRRLGLDERGVKLLGEVPQGLPDLGLPALHWEDLNAMLPLALACFLLAAV
                     ETAAIGRTFAAKYDRRLDANQEFLALGASNLAAGFGQGFPVSGGMSQSVVNEGGGART
                     PLSGAIAAVFILVVVLFFSHLLSALPQPVLAAVVLVAVASLVSLSALRHLWRSDGREF
                     VIAMVAMFGVLGQGLLRGVAIGAVISLVLLIRRAALPPVALLGRIPGTRRFSDRERHP
                     ENETIPGMLILRPGASLLYFNMNYVRDTIEERVRAEQETKLVVLDLSEVSIVDMHAAE
                     MLAGLAGELAAKGIKIQAVEAHSQVRDRLRSEGLNERFGGIERLRTVADVADDFLRGG
                     LGQEDVHVALGDLGAVPRPGRTAKPAAGADQSRDRPGRQGWKRRGDNGKR"
     gene            19535..20377
                     /gene="ppk2"
                     /locus_tag="VFC01_18880"
     CDS             19535..20377
                     /gene="ppk2"
                     /locus_tag="VFC01_18880"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005176929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HZN81726.1"
                     /translation="MSKGGKKSGEKSARKIPKNVYQAELFRLQTEFVRLQEWVRETGA
                     RIVIVFEGRDAAGKGGTIKRITEYLSPRVARIAALPAPTERERGQWYYQRYIAHLPAR
                     GEIVLFDRSWYNRAGVEKVMGFCTPQEHSLFLRQTPIFEQMLIDDGIILRKYWFSVSD
                     EEQLRRFRSRRNDPVRQWKLSPMDLESIFRWEDYSRAKDEMMVHTDIPMSPWYVVESD
                     SKKHARLNMMAHLLSTIDYEDVEAPAVELPAHPVRSGNYYRPPRELSTYVDDYVATLV
                     GDPE"
     gene            20854..21096
                     /locus_tag="VFC01_18885"
                     /pseudo
     CDS             20854..21096
                     /locus_tag="VFC01_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
     gene            complement(21156..21428)
                     /locus_tag="VFC01_18890"
                     /pseudo
     CDS             complement(21156..21428)
                     /locus_tag="VFC01_18890"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(21475..23922)
                     /locus_tag="VFC01_18895"
     CDS             complement(21475..23922)
                     /locus_tag="VFC01_18895"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZN81727.1"
                     /translation="MAKTSKTAKPVKKASTKKSAAPKAAPAPVAEVPSTAPLSRQHLP
                     IPDPKHVGLTTYDAKDPNTKYPPITMLRPPAGAPNVLIVLIDDVGFGASSAFGGPCNT
                     PVAERLAANGLKLNRFHTTALCSPTRQAMLTGRNHHSVGMGAITEMATSAPGNSSIRP
                     KEKAPIAETLKLNGYSTGQFGKCHEVPVWEVSPAGPFHQWPTGSGFEYFYGFVGGEAN
                     QYYPGLYEGTTAIEPPKSPEEGYTLTEDLANRAITWVRQQKALMPDKPFFMYFAPGAT
                     HAPHHVPKEWSDKYKGKFDDGWDALRDNTLVKQKKLGVVPKDAELTKRHDEIPGWDDM
                     PSELKPVLARQMELYAGFLEQTDYEVGRLVDAIDDLGVLGDTLIYYIIGDNGASAEGT
                     LNGCFNEMTTLNGMPGIETTEFLLSKIDDFGTPKAYNHYAVGWAHALCAPYQWTKQVA
                     SHWGGTRNGAIVHWPNGLTEKGNTRNQFHHVVDVAPTILEAAGLPAPQFVNGIAQAPL
                     EGVSMLPMLRDGAAPETHEVQYFEMFGNRGIYHQGWTAVTKHRTPWKADQPPPFDDDT
                     WELYGPDDWTQAHDLAKRNPAKLAELQRLWLIEAVKYNVVPLDDRGFERVNPDIAGRP
                     QLIRGNTQLLFDGMRVSENCVLTLKNKSYSVTANIVVPESGADGVIITQGGSVGGWSL
                     YAHEGKLKYCFNFFGIEHYITSAKKPIPKGKHQVRMEFKYDGGGLAKGGDVALYYDGK
                     AVGSGRVEQTQPMGYSADEACDVGSDTGSPASPDYGPTGNRFTGEIEWVQLDIGDDSH
                     DHLITAEERFNLAMARQ"
     gene            complement(23959..26325)
                     /locus_tag="VFC01_18900"
     CDS             complement(23959..26325)
                     /locus_tag="VFC01_18900"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020731863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZN81728.1"
                     /translation="MSLTEYKPGSAFPGRMGRTVGESSPAWPAPVRSKPGAPNVLYIV
                     LDDTGYGQFGCYGSPINTPNLDRLARNGLQYTNMHTTALCSPSRSCMLNGRNHHSNGM
                     ACITEGSTGFPGSNGAIPFENGFLSEMLLPHGYATFCVGKWHLTPAEQISAAGPYDRW
                     PLGRGFDRYYGFLGGDTHQYYPDLVFDNHQVEPPKTPEEGYHLTVDLADKAIEFIADL
                     KQVAPDKPFLLYFATGANHAPHQVPKEWADKYHGKFDDGWDAYREKVFARQKELGIVA
                     KDAVLSRHDPDVQQWDELSADERRLYARMMEVFAGFFEHTDHQIGRLLDFLEAIGQLE
                     NTLVMVISDNGASAEGGPHGSVNENKFFNNVPDDLQQNLAALDELGGPKYFNHYPWGW
                     TFAGNTPFRRWKRETYRGGVSDPFLVHWPNGIKAKGEIRNQYAHAIDMVPTVLESLGI
                     EAPSELRGVAQSPIEGVSFAHSFDDAGAPTNHHTQYFEMFAHRSIYHDGWRAVCPFPG
                     TSFAEAAVSFGMLELSEDKLRELDATGWELYNVEVDPAETKNLADQERAKLIEMIALW
                     YTEAGKYNVLPLDSRGTMRFADERPQIAAERDTYVYFPRTQAVPENVAAKVLNRAHTI
                     TVDAELKTGDEGVLACHGSNVGGYVFFVQDGKLHYVHNYVGAEELQVSSDQPVPPGKH
                     ALRYEFEPTGAPDLKNGKGTPGIAKLFVDSQLVAQADFPVTVPLALGLGSGFAVGRNP
                     GSSVSVMYASPFPFTGRITKVTVDVSGKPDHDEDEVKQSHARIAMARQ"
     gene            complement(26706..>27328)
                     /locus_tag="VFC01_18905"
     CDS             complement(26706..>27328)
                     /locus_tag="VFC01_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003904168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN81729.1"
                     /translation="AIAVRIIRLLGGPEARPNVISFMHDQQGPDAAYRETLGCRVRFR
                     QTWCGFELPHRLADRRIESADPETRRIAAKYLESNYLPRTASLSDRVAELTRRLLPTG
                     QCSVDAIADQLAIHPRTLQRRLATEGVRCQDLVERERRTLAARFLAEPQLHLNQIAGL
                     LGYTEQSTLNHSCRRWFGKTPRQYQRDLQSRALTRPEPPIAVYSHE"
BASE COUNT         4595 a         8570 c         8960 g         5203 t
ORIGIN      
        1 tcgacgacga catgctgctg gcgtgggacg ttccgtgttg ccgttgagtg tgtttggtga
       61 caccccgggt cggattccgg cgaagtgcca ggtaggggcg tgtctgttgt gggattctgc
      121 gagggtgtcc gctgaccggg cagctgctcc ttcgggggtg gtgacgtttt tgttcaccga
      181 cattgagggt tcgacccgtc ggtgggaggc cgttgccagg tcgccaaggc gcgacatcct
      241 ggaacggcgg gctcgggaga acgccgtcga cttcgtcgag ggtgtggcca ccgcgctcac
      301 gcccattgcg ttcagacgtt catggtgaaa agttatgaga cccttgtcga ctcggacacc
      361 agggtcgatg tgaacaccgg catcgtcgcg gcgagtcggc tccaggccgt gatcgagccc
      421 agggactaca gcatcgatat cgccgagatg gtggcacgga ttccacggcg ccaggcccga
      481 tgtgcatgag cagcgcgcct gacgtgtttt cgctgaccac accggcatcg gccgaactgt
      541 caccggtcac ggtttccagc aatgggcagt tcggattacg tcgatgaacg tcattctgtt
      601 cgtgttcgcc ggccgcaagc agaacatgga actccagctc ccgctagtca ggcgcatcat
      661 tactgagcat cccaatgtcc aatatcacgt gtggaatctg tcgcgtaatg acgacgacaa
      721 tctttacctc cgagccatcg agggtgatcg catcaacgtc ttgaatgact attttggcat
      781 cgccccgtgg ttgggattca atgccgtata tcgtcactac gctcacaacc ccaagtaccg
      841 ggatcaccta ttcgtgaagg tggatgatga tgtcgtattc attgaaacgg gccggttcgg
      901 ggagttcgtt gaggccgtga acggcaatcg cgcgtcagtg gtgaccgcga aagtcgtcaa
      961 caacggggca tgcacgccca cggagccggg gttgtggagc ggcttcgaag ccctttcctt
     1021 gccgttgttg gatgttcacc ttagcggtga gtacgccgtg atgagccaca cctacatgtt
     1081 caagcactgg cgcgaaatgg tcggccaacc agttaaactc atcaagaccc gcgactggtt
     1141 gtcaatcaac acgattggct atgactggca tatggtccgc aaaatagcac gccgcatcgg
     1201 gacaccatcg ccgggggata tcgcgggccg cagcttcacc cccagaatca agatcggcga
     1261 tgagggcatg gtgaacatgc tgccgcgctt gatttttcaa gggtttctga ccgctcatct
     1321 ttcatttggc ccacagcacg cccatctgac cgatgcccag ttgtcactgt ggcggaagcg
     1381 ttacgccgca atcggacagc agtatctcga cgcatgaaag tcgccatcgt catccctttc
     1441 agagaccgcg gtaatgaccc actgcgcccg gccaatctga ggtgcgtcat cgagcattgg
     1501 gacggctgcg atgtgcctgt ctggctcagc gacgacgggc gggtgggaga cgcacagttc
     1561 aacaggtcgc aggcctacaa ccgcggcgcc tggttcgtag atgcggatgt ggtcgtgttc
     1621 gcagaggccg acatgctggt gccctacagc cagatacggc aggccgtcgc gatggcggca
     1681 gcggccccgg gtctggtggt gccgttcatc cagtaccgct acctcacacg cgaagactcc
     1741 ggcctggtcc gcgcgcacga catcgagccc ggcgactgca tcccggaatc catcatggac
     1801 aacggcgaga gcatcggcgc catcaacgtc gtcagtcgcg agacgctgga cgcgatcggc
     1861 cagtgggatg aggtcttcga gggggcctgg tacgacgacg tggcgatgaa gatcgccttc
     1921 gaggtgtgcg caggccccac ccggtgggtc ggtggcccgg cctggcacct ttggcataaa
     1981 ccaggtttgg gtgggtcgca actaaccgca gaagacaagg ctgctactgc ccggaataaa
     2041 gagcgattag agctatatcg acaggccacc acccccgagc gtatccgcga gctgactgcg
     2101 ggtcggctct agcttcgctg gggtgtcgat gaaggtggcg aagtaggtca tgtccagcgc
     2161 cagcccggtc gggtcgagcc cgaactcggt gaccatgcgg cgggcaaggc gggtctcgat
     2221 ctcgcgcagc tgcccctcgc cgaggcggtc catcgcgtcc cagaaccgac ggtgatccac
     2281 cgcggcccgg ttcagccgca cccaccgcgg ggttgcgatc aggacatgca gtgaagctac
     2341 tgctatcccg gagggacttg cggcggttat cgcggaccgg cctgaacgtc gaccttcgag
     2401 gcgctgaaag tgcttgccgc acagactgat cagcggttag acaggattag acgcgggcga
     2461 gttcgccgcc ttccaccctg ctcagccgcc actcaccgtc gccgagcagt tccaaatgcc
     2521 cctcgtgatg ctggccgaca gtgctgcggt gggtgacact gaccaggact gtgtccggga
     2581 gctcggtgcg caccatgtcg tacagcatga actccaagcc ctcgtcgagc gcggatgtcg
     2641 cctcgtcgag gaatgccacc ttgggtttgg tcaacaggat gcgcgcgaac gcaatgcgct
     2701 gctgctcgcc aggcgagagc acgttggccc agtcggccac ttcggagaga cgctgtgagc
     2761 actgcggcag cgccaccttt gtcagcacac ggtgcagttc ctcgtcggga atctcgccgg
     2821 gttccttggg gtaggacacc accgcgcgca ggtcaccgag cggtacatac ggcatctgcg
     2881 acaggaacat cgtctcgttg gcgccgccgg ggcagcgcaa ggtgcctgtg gtgtacggcc
     2941 acagttgggc caggctgcgt agcagcgtgg tcttaccggc gcccgacttg ccggtgatga
     3001 tcaacgtgtc gccaaggttc agacgcagat ccagcggccg gatcaactgc tcgccggccg
     3061 gcgtacgcac ctcgacatca tcgagttcga ccaggcagtc cctgctgggc gtgacggtca
     3121 gcgacggcag cgcgcgaccc tcctcgttgg ccgtcaccag cccgtgcaag cggatgatcg
     3181 acgcgcgcca gccagcgtag ctgtcatagg cgttacggaa gaacgacagt ccgttctgga
     3241 tgttgctgaa cgccgtcgcg gtctgcgtga cgtcgccgaa cttgatctgc ccggcgaaca
     3301 gccgcggcgc ctggatcagc cacggcggca ggacgatgag ctggctgatt gaaaagttcc
     3361 agccgaagaa gcggatcatg cggttgatgt agcgcttgta gttcgacacc accggttgga
     3421 atcgccgatg cagctgcaac cgctccgcgg cttcgccgcg gtagaaggcc accgattccg
     3481 cggcatcccg caaccgcacc agggcgtaac ggaacgcggc gttgtacttc tcgttgttga
     3541 agctcagcca gatgatcggg cggccgatcc ggaatgcgac gatggacgcg aggaggacgt
     3601 acgcgaaacc gatccagaac atcgccctgg gcatggtgac gccgaacagc gtcaagttcc
     3661 cggacaggcg ccacaggatc gcggtgaacg agatcaccga gacgacggcg tcgatcgcgc
     3721 cgaacagcag cgtgtttccg gtggtgttgt tcggggtgtt gggcagcggc ccgactccgg
     3781 ctgtgaagat gtcgatatcg gactggatgc gctggtcagg gttgtcgatc cgctcgtcga
     3841 tgaagcgccc gcggtaatac gcctggccgg tcaaccagtc accggtcagc cggtcggtga
     3901 gccagacccg ccaggccagc atgaaccgct gcatcatgaa caggtcgagc atgacgcggg
     3961 cgatgtgcac ggtggccagg atcgagaaga tgcccagcga ggtataaaag ccgtgcttgc
     4021 cagattcttt gaccgcttcg tcgcctgcgc cgatgccggt gaacgcggcc tgcgccgccg
     4081 acatcatgtc gttgccctga tagctgaaca acacgttgag gcgcaccccg acgatcaccg
     4141 acagcagcag tgccgccagc cacagccaca ccttcacgct gtgcgggccg gtgaagtagg
     4201 cgccggtgat gcgccagaac tgccggcccc acgtcgtata gcgcgcaatc agtaccagca
     4261 ccaccagggt gcacaccgcg gcgatcgccc acgccctggc gatccagatc gccgaatcga
     4321 acagcgcgcg cccccagtcg atcgagaacg taaacagatc gttcaccagt gacttctcct
     4381 accccgggga tctcccgtac tggcaactct gcaccacatc atcggcgtgg ttccaggccc
     4441 gcgacgtcgt ccgcgccggc ggcgttgccc gcatagagca gggtggtgtg atcgaggccc
     4501 agcagggcgt acaaccggat ggggtcgctg accgcctggc gggtcagccc tctggcgtcg
     4561 ggcgcgcggc cgcctccgtt cccagtccga cgtggctgtg acgcgtcagc gcacgagcaa
     4621 gcagggcggc aaggccggct ccgcacgcca caccggctaa ctagccggac ccgtcgaggc
     4681 cggtggtcgc tgcgaggaca ccgaagagcg cgagcaggca gacgaagccg ctgagcgggc
     4741 cggtcgggac ccggctgcgc acttgaccaa cccgacgcac tgtggctccg tatctcaggg
     4801 gtacgaacag tcatacggta aaggcgacgc ttcggttcat ccgggcggga agaggagtat
     4861 gcgggtgcct acggaagcgc gcgcgttctg gatcctcgag cccggccggg gcgagattcg
     4921 cacggtcgcg ctgccggagc ccggcccggg cgaggtgctg gtgcgcacgc tgcggtcggg
     4981 gttgagccgc ggcaccgagg gactggtgtt ccgtggcgga gtcccgcaga gccagtacga
     5041 cgtgatgcgg gcaccgtttc aggagggcga ctttcccggc ccggtgaagt acggctacct
     5101 caacgtcggc gtcatcgagc gcggtcccga cgagctccag gggtgcaccg tcttctgtct
     5161 cttcccccac cagacggcgt acgtcgtgcc tgccgacgcc gtggcagtgg tccccgacga
     5221 ggtcccgccg gagcgtgccg tcctcgccgg cacggtggag accgcggtca acgccctatg
     5281 ggacgccgca ccgttggtcg gcgatcggat cacggttgtc ggcgccggca tggtcgggtg
     5341 ctgcgtcgcc cggctggtcg gtggcgtgcc gggaacgcag gtgaccctcg tcgacgtgga
     5401 cgacacgcgt gcgcacatcg cagcctccct cggcgttggc ttcgcgctgc ctgagcacgc
     5461 cctcggcggt caggacctgg tgttccacgc cagtgcgacg tcggccggac tgcagctctc
     5521 cctggccctg ctcgctgcgg acggcatggt cgtcgacctc agctggtacg gcgaccagcc
     5581 ggtccagctg tccctcggtg gcgccttcca ctccagacgc ctcggcatcc gggccagcca
     5641 ggtcggcgat gtcgcggtgg cccggcgcgg ccggcgtacc acgggtgagc ggcgcgcgct
     5701 cgcactggac ctgctccagg atccggcctt cgacgcactg ctcaccggcg catcgacgtt
     5761 cgacgagctg cccgacgtga tggcccgact gtccgacggc cggctccgcg cgatctgcca
     5821 cacgctgacc tacgacgaag ggtagggccg atgttcagtg tcactgttcg cgaccacgtg
     5881 atgatcgcgc acagcctccg cggcgaagtg ttcggtcccg ctcagcgcct tcacggagcg
     5941 acattcatcg tggacgcgac cttctcccgg gtcgagctcg actccgacga catcgtggtg
     6001 gatatcggcg gggccaccga ggtgctgcgt gacgtcctgg cggacctcaa ctaccgcaac
     6061 ctcgacgagg tgcaagagct cgcggggcgc aacacaacca ccgaggtcct cgcgaagctg
     6121 atcgccgatc ggctcgccgg ccgtgtcgag ggcggcgggc tcggggactc cgcgagtggg
     6181 ctggcccgga tcgcggtaac cctgcgcgag tcgcacatcg catgggcgag ctacgaaaga
     6241 tcgttgtgag cgaggtgcac gtcgtggtcc ccgacggaat cgacgacccg cggcggccga
     6301 gcggcggcaa cgtgtacgac cgccgggcct gtcgcgggct ttccgcgctt ggctggtcgg
     6361 tccacgagca cacttcgccc ggccgatggc cccggcccga cgggcaggcc cgggcgggtg
     6421 tggctgcggc gctggcccga atgccttccg gcgcactggt actcgtcgac ggcctggtcg
     6481 cgtcggtggt gccggaggtc ctggagcccg aagccggtcg gctccgcctc gtcgtacttt
     6541 tgcacatgcc gcaggacgag ccgcgcgagg tcgcggcgct gtcggcggcg tccgcgatcg
     6601 tggccacgag ccagtggacc cggcagtggc tgagcgacaa ctactcgctg cccagcactc
     6661 ccgtgtacgt cgtgccgccc ggcgtcgacg cagccaaagt ggcagcgggt accccgtctg
     6721 gtgggcggct gctcagcgtc ggcgccgtca ccccggtcaa gggccatgac gtcctgctcg
     6781 acgcgttggc cacggtcgac gaccccgcct ggcgatgcgt ctgcgtcggc gcgctggacc
     6841 tcgaccagga gttcgtcgcc cgcctgcgat ggctggccga ggacagcggc ctcgacgatc
     6901 gggtgtgctt caccggcccg ctcacgcacg acgagctcga ccgcgcgtac gcagaggcgg
     6961 acgtcctggt ggtggcctcg cgctcggagt cttacggcat ggtcgtcacc gaggcgctag
     7021 cccgggggct tccggtcatc gccggggagg tcggcggcgt gcctgaggcc ttgggccacg
     7081 cagccgatgg cagccggccc ggcctgctgg tcccgcccgg cgacccgcag actctcgggc
     7141 agacgttgag tcgctggctt ggtgaccccc gagagcgcga gcggctccgc gcggcggccc
     7201 ggtcccggcg acagagactc acggactggg cgcagacctc gcgcgagctg tcggccgtgc
     7261 tgttcgaagt cgcccggtga ccggatgaac cagcagcagg tgaccctcgt atgactgtcg
     7321 cgggatatac agcgaggaga tcggaggcac cgccatcagc cgaagcatct ggacatgggc
     7381 acgcctggcg ggcggcgccg cgatcctcgc cgtcctggtc tggcgggtgg gcgcgggccc
     7441 gttcgtggat gggggtcctc gacgccgagg cgtaaggcac ggactgcccg tcgacttctg
     7501 agctgtcatc tcggcaccac caacagatcc tggtggtgca cggtgagata caggtggccg
     7561 gcggctgcct cggccaggcg tcgccttgca tacgacggag catgggcccg cagctcgggt
     7621 cgctgctcgc atgcgccggc cagccagccg gtgaaccacg cggccgcgag agcggcgtga
     7681 cccgggccga gccgccacgg gctcgggcgg accaggactt cgcggcccag ccgggtgaag
     7741 ctgtcgaccg cggcgccgac cgcgtcggga ccgagcagct ttccgacgct tgtggtccgt
     7801 cgctggtggg cgttgaacgc gtctctgacg ctttggtcga acggctcggc cggcgtgatc
     7861 tcgacgcggc ctgtgacgct gagggcgatc agcaccgggc agtccgcccc ggcacaggtg
     7921 gcgacgagtc gttcgagctc gtcggcggtc atcgtgtcca gcagcgccga cgcggtgatg
     7981 agagctgcgc ccgccagctc cgcgagctcc atccgggtga tgtcgcgccg acgggcctcc
     8041 atcgtgaccg gcgtgccgtc ggaggccctg tcgggcgggt ctgcggcagc gagcgtcagc
     8101 agctcgtcgt ccctgtcgta catcacccag tgttggggtc cgggcagccg caccgcgagc
     8161 cagtgggcca tcgacccggt gccgcaaccg aggtcgtgaa tggtcgcccc gccgtgggtc
     8221 ggcaggtacg accggacctt ctcgacgagc tcggacgcgc gggctgccgc gtcggccggc
     8281 tcccgcaacc tcagccactc ggagctggcc cgcaccggtt cggtcgtcat gccgcctccg
     8341 ccaacgcctg acctctcacg agccgctcac ctcgccctcc aggtggcgcg cgagacccgc
     8401 ctcacgccga ctgccgcctc atgcccagcg cgatgccgat gcgtgcccag gacatgccgc
     8461 ggtcgcgcag ttcgcgcacg cgatcgcgaa gtcccgcttc gatattcgtc gccgatgtgg
     8521 acgcgatccg ttccagtagc tcgtcctccc acatcaaggc ccactccgat agcgacggcg
     8581 gctcggcggg tacggcctgg ccgacgaccg caacacactt gtcgacgtag gcgtcataga
     8641 tgcagatgcc tgcccggcga tgagcgtggc aacccccacc tgggggagaa caccgacttc
     8701 gaggacgacc gcgtcggacc cagcctcgca atcagcgacg ccgccgccaa gcaaatcatc
     8761 gaacacaaac agcatatgcg ggaacgcttc ccggacacag tgctggccga ccttgcgcta
     8821 ctgccatgag ccggcatgtt ggcgccgtgg ttcaccgatc cggcgaggtg gcggccctcc
     8881 ctggccgcct tgccatggcc ctccggccca cgacgcagcg tacggccagg cagtgaacgg
     8941 ttgtccaccc cacgtcgtgg ataacgtaag cggaccatcc catggccgcg cgagcggaag
     9001 gacttctcta caccaagggc caacacgccc tcgccctgaa agggctgaca ctgcaatgag
     9061 atcaacggtt atcgcggtgg cgattcggct ggccatcagc gcctcgctcg cggtcagcgg
     9121 cgtcattcac gcatacctct atgtcaacgg ctaccgcgac atccccatcg tcggcccggg
     9181 gttcttgggg caggccatcg tcttttgtgt tctggccctg ctgatcctcg ccggcggacc
     9241 ggattggctg cggtgggtcg cgggcgtgct ttccgccggc gcactggtcg cgttcgcgct
     9301 gtcgcgcacg gtcgggctct tcggattcgc cgaacgggga tggaacccat caccgtacgc
     9361 ggcggtcagc gtgatcgccg aagtgctcaa cgcggtattc gttgcggcct ctgtgctgag
     9421 cagccgcacc aaaaccccat taccctaaca gcgctcacac caggccgcgg gtgtgtcaac
     9481 ggcacaatcg ttcgctcaac cagagcatcg atcagcttca ccgatccgcc ggaatcgttg
     9541 ccactgtgcc gttcgggcaa cgcgaaggcg ggcgggtgat ccacgtcccg gcggtcgtct
     9601 ggattagctc accgagcacg gcttgaagcc aatgacgttg ggcatcaata ccagtagcgg
     9661 cggccaccga ctgggcggcc cattcggcct agtaccaaca gcacgagacc gatgacgagg
     9721 aggatccagc cgatagtggc gatgattcct ggcacaggaa gtaggtagcc gacgaggatg
     9781 aggatgacac cgagaacgat cacggattcg gctccttccg tgcgtgaacc acccgcggca
     9841 taccccggga accgactgcc gaaacgcggg accaggcaac gggccgccac ccatacgggg
     9901 atagcaggcc gcagtcggtc cgaaatgccc tgtcttacag catttttgca gctatcgccc
     9961 aggtgcacta caggttggtc acacctgggc gggcatcgga acatctcatg caacacacaa
    10021 ttccgaggga gatcggtatg ctgcccggcg ggttacgccg accggttcca caccatcgcc
    10081 accgattgac cgaaacctgc ctcaccgaag ctactgcgcc gagccgggcg gatcctgggt
    10141 ctcgccgtcg ctgacgcaat cagcctgttc gacccgagcg tcatttccgt ctgcggccaa
    10201 ctcgcttgga actgaagaga acctctcgct gggatccgcg aaatcgttta ccaactgtcg
    10261 ctaccccggg cgacccgtca tcttcaaatc gtgcgcagcc aactcgatcc tcgcccgggc
    10321 ttctcggact ctcggtactc ctgtcggaag aaatcttctc cattgagcgg tcggcatgct
    10381 ggcacaactc gcggctccgg ttggcggcag ttgggtgttt ctggttcggc attgtcgagc
    10441 agcggtagac cagcaaacac cctcggcacg acggccagct acgtggacat gttggactcg
    10501 ccaacaactc cggcgtaatt cccaggtact ttcgcggaat ctgccgtgct ttcgcgtaac
    10561 cctacgcagg tcaagccgca tttccgcagg tcacgtcacg ggttcgattc ccggcagctc
    10621 caccgaaagg gccgcagaga tgcggccctc acttcttcgt tcagtcgcgc gcaaggatgg
    10681 ctccgagtcg ccgttccagg tcgacgtatg catcgtcgcc gacgtcgtag gtcaccttcg
    10741 atatccgtcc gccggtaaat gagaacccgg gcgaatactc ggtcgatacg gggtcggcgc
    10801 tgtcacgtcc gacggccacg ccctcgccgc acagcgcgta atggccgctc tgtgttcgga
    10861 agtccgcctt cgcgacggcc ttgtcgtcga tgtagagggt catcgtcccg agtacctcga
    10921 gcttctcgct gatcgaactc ttggcaaagt caactcccac aacatgtttc cccgacacgg
    10981 gcgcggggca actcagcttc tgctccggcg cgatgccgag gaagttgtag acgaggttca
    11041 gctggccgtc ctttacaaat agcgtgtatc cgccgaagcg ggaaccttgc gagacgatca
    11101 cgccctgggt cgccttcgtg aactcgacct cggcgaggat cttgaatgat gccccgagcg
    11161 ttctggcggc cgaggcctcc ggaacttcgc tcgtgtcggg gtagtaggta tagcgaccgt
    11221 cctcgggcgg cgtcactttg tactccaggg cgtgaatccc ttccacgcca tagtcgttga
    11281 ggggcagggc gttgttgctt ttcgcttcgg ctagccacat ctcggacagc tgcttgacct
    11341 tttcgggatg ctcggctgcc aggtcgtggg cctcggagcg gtcgacgtcg gtgtggaaca
    11401 gttgccagcg gtcgtcgtcg aaggcgcctt tgttgatcat ggggccgtgt tcggtggaca
    11461 ccttccagcc gttgtgccac aggccacgtg tgctgagcat ctcgaagtac tgcgtttcct
    11521 tgcgggtggg aacctcggcg tcgtcgaagc tgtagcgcat cgacacaccg gctatgggcg
    11581 cctgccgaac gccatccacc gcgtcgggca tggtcagacc gcaacattcg aggatggtcg
    11641 ggacgacgtc ggtgcagtgg tggtactggt cccgtatttc accgcgggcc ttgaagcctt
    11701 tgggccagtg gatgaccatc gggtcggccg tgccacccgc atattgggag taccgcttga
    11761 acatgcggta tggcgtgctg aacgcgacag cccaacccgt cggataatgg ttataggtct
    11821 ccgcagagcc gagtttgtcg atcatggcca ggttggactc agtgtcgtcg ggatatccgt
    11881 tgaagaaccg gttctcgttg accgacccgt tcgggctgcc ttcacccgat gcgccgttgt
    11941 cggcgcagta gaagatcagc gtgttctcca gctgaccgga ttcttcgaga tagtcgatga
    12001 ttcgcccgat ctggacgtcg gtgtactcgg aataagcggc gtagacctcg gccatccggc
    12061 tgaaaagcgt cttctcgtct tccgacaacg acgcccaggg gcggacactg tcgccctcga
    12121 tgaatgtgcc aggcgtcatc ggattgagtt ctgtcagttc ggtattgggt ggcagcacgc
    12181 ctttttcgat catgcgcgca agtacccatt cccggtatgc ctcgtaaccg tcgtcgaatt
    12241 ggcccttgta cttttcgatg tattccttcg gggcgtggtg tggtgcgtgg ttggcgccgg
    12301 ggcagtacca catgtaccaa ggcttgtccg gtcgtgattg cttggtgtca cggatgaagc
    12361 caattgcctt gtcggcgatg tccttcgaga agtggtagcc gtcctcaggc gagtacggct
    12421 gctcgatgaa atggttgtcc tcgatcaatg tcggatacca ctgattggtt tcgccgccga
    12481 tgaagccgta gaaccggtca tagccgagac ccagcggcca gtgcttgcgg ttcgcgccga
    12541 cgtcgaaggc atcgaccggc acgttgtggt tcttgcccac ccagaaagtg ctccagccgg
    12601 catcgcgaag caccgtcgcg atcgtcgcgt tttcaggcgg aatgtgcccc gagtagcccg
    12661 gaaagccggc cgcggactcg gcgattgtcc cgaaaccgtt ctgatgatgg ttgcggccgg
    12721 tcaacatcgt cgagcgggtc ggcgagcaca ccgaggtggt gtgccattgc gaatacgtca
    12781 gcccgttcgc tgccagccgg cggagcgtgg gcatgctgat tcttccacca taggcagacc
    12841 acgccgcaca acccgtgtcg tcgtagagga tgacgagcac attcggcgcg ttccccggag
    12901 ccttcgggtc gagaaaggca ttccagtctg gcgtggaatc acggatgtcg agttcgatct
    12961 tgccgttgaa ctgccctgcc atgatcactc ctcatgcggg tcgtctaggt gcgttgtacg
    13021 ttcggtgact tgtacgtgcc gtcgagcagt gactgtgtcg gcacgtagat ccgggcgagt
    13081 gactcgacgt acttctcgtt ggcgagcctc gcgggaacgc cggctgcgtt cgcaaggtcg
    13141 ggatgcgaag gggtgaatgg tgtggccctc gtcaccagcc caacgctaga agcctcgccc
    13201 ccaccaggtt ctcattcgac attttccagc gtcaccgccg cgtgctctcg cggactgatc
    13261 tcaaggcggc agattgggcg gcaggtgttg acgccgtcaa catctttcct gtatccgcag
    13321 gtgatttccc gtacttttgc gtcgtttcgc gtgctgaagt ccagtatttc cgcaggtcaa
    13381 ctgcatttca gtaccggttt cgattcccgg cagctccact actaaaggcc caggccaggg
    13441 aaagattccc gagcctgggc ctttctacat cgatgcacct atcaacaatc tgggctcgcc
    13501 gacatgaacc tgcacggagc cttcgaaccg ctggacgaat acgcggagta gccgtggcta
    13561 gataacgctt cacgctgagc ttcgaggtca tcttttgcga cgttgcgagc tatggactgc
    13621 tcccttgtaa acgtcatctt ttgccatgga gaaggacctg caaagcctca tttggactcg
    13681 tcgcgacact ggacgagttc cgaaagctcg gcctgaccag tttcaagagc aagcaagtca
    13741 gctttgggat catctgctgc gggtccgcgt cccctccgat cacggggcgc atgatcttgc
    13801 ccgactgcag caggtcagcg cgtacatgcc gcgtaaggtt cgaccatgaa cgtcgggggc
    13861 ggggcgcaaa acatccacta tgccaagagc ggcgatctga acattgcgtt ctcggtgacc
    13921 ggagaggggc ccgacctcgt cgtcgcgccg ggcttcatct cgcacctcga catcatgtgg
    13981 gaggagccgt cggtggtcca cttctattcg cggctcgcct ccttccggcg cgtcgtcacc
    14041 ttcgacaagc ggggcactgg cctctcggat ccggcgatgc acgcgccgac gttggaagag
    14101 tcggtcgatg acctgcgcgc ggtcatggat gcggccggat gtgaacgcgc cgacgtcttg
    14161 ggcatttcgg aaggtggcac gatggcgata ctgatgacgg ccagccatcc cgaccgcgtg
    14221 aacgcgcttg tcttatacgg cacgttcacc cgcctgctcc gagcgcgcga ctatccgctc
    14281 ggcgtcaccg atgagcagct gtcggcgttg gtcgagctca gcgccaaggg ctggggtgag
    14341 ggcgtcggac tcggtggatg ggctccgagc cggcgcggcg acgccgacct gcggcggtgg
    14401 tgggcgcggt tgcagcgcgt ggccgccagc ccgggcatgg tccgcaacat attcgcgctc
    14461 tatccgcagc tggacatccg cgaagtgctg cccgcgatcc aggtgccgac gctcgtgctc
    14521 caccgccggg acgaccggat ggtgagcatc gagatgggtc gctacctcgc cgaccgcatc
    14581 cccggggcca agtttgtcga gctcgagggc gccgaccacc tgttcttcac ggacgacgcc
    14641 gacaccttgc tcgacgagat cgaggagttc ctcaccggtg tccggccgct gcctgcggtc
    14701 gagcgcgtcc tggccactgt gctgttcacc gacatcgtcg actccacgaa gcgagcggtc
    14761 gagctcggcg acgagcggtg gaaggagctg ctcggcaggc acgatgccca ggttcggcgc
    14821 cagctcgagc gcttccacgg ccgtgaggtc aacacgacgg gcgacggttt cctcgcctgg
    14881 ttcgacggac ccgcccgcgc gatccgatgc gccacggcga tccgcgacgt gctgcgggga
    14941 ttgggcctcg aggtgcgggc gggtgtgcat acgggcgagg tcgagctgcg cgacgacgac
    15001 atcagcggta tcgccgtcca cgtcgccgcg cgcgtggcag cagcggccgg ggcgggcgag
    15061 gtgctcgtct cgcgtatcgt cgtcgacctg gtcgccgggt cgggccttag ctttaccgct
    15121 cgcggcgagc accctttcaa gggtgtgccg ggcgagtggg cgctgttcgc ggtcgagggt
    15181 tgacgcggtc gggctgcgag tcccacccgg gttacggctg gaaaccgacc gtggccttgg
    15241 cgagggaaag tgtcactgct caaccctccc cgcaagggca agacgatgat gaccgccgtg
    15301 atggtgacct acgcatacga cgaaatcgac caggcccgag ccgaactgaa cgtggtctca
    15361 aaatagccgg gagctagctt tgccagtcgt tggagccgtc gtttccggtg taggtgccgc
    15421 cgctggagtt ttgcaccacg atcggatcac cggcgttgaa gttgtcgaag aaccatttcg
    15481 cgttggcggg gctgatgttg atacagccat ggctgacgtc gcgcttgccc tggtcgtcca
    15541 ctgaccatgg agcgctgtgt acgaagtcgc cgctgtcgtc gaagcggacg gctagctcca
    15601 ccgtcgtcct gtatccgtac gtcgagttga ccgggacccc gtaggtcgag gaatccatca
    15661 ctaccgacgg catcttctct tggacgtagt aggtgccgtt tggggtttgg tggccgccgg
    15721 cggtcatacc catcgacatc gggatggtct tctccacggc cccgttgcgg gtaacggtca
    15781 gttggtgcgt ggcgtcgtcg gccgtggcca ccagcgtgtc tcccgtctgg aagctggaca
    15841 ctgtgccgcc cgcgtcgacg gtcaccgcgg tgtgggcggg ccagaagttc agcgggcgcc
    15901 agcgcagctg cgtgggcgtc atccagtaga acttgccggg aaccggcggg accgaagaga
    15961 cgcggacggc gttttcggct gcgccggcgt catcgaccag tccggggaag ttgatgatga
    16021 tcggctgggc cacacccacc gtcgagccgt tggcaggggc gatcctcggc ggcccgaatg
    16081 gcgccgtgcc ggtgaacggt gtcgggtcct gccccccagc ggggcccgcg acaactggta
    16141 ccgccagcgg gtcagggcca ggcggcaacc cagggacggg cggcgcgaca acccccggtt
    16201 cgccgggagc cggattcgga tccggatccg ccagcgcggg acccgcactc agcaccagca
    16261 cccagcccaa tatcgaagcc gctcctgctg tcttcagagc ggcgaagagg cttcccctcg
    16321 tccagcccga catatgcatc cctccagtca ctttctggaa acagtgtgtc gcatagcgcc
    16381 caggccagct atcttttccg ccaccagcgg cggtccgcaa ctacttgatc gaccccaacg
    16441 cagtggtgtt gcggcaagcc gagggccggg gtgggtatgc gcgaactgcc gaggactgca
    16501 cctcacgagg ggctgtgggt tgtgtatgcg ctgcgcgcgg cgtttgcagg aacaggcagt
    16561 tcctcgatgc ccctcgtgaa gctcatcccg cgtgttgatg tcatcaacac ctttcgcgta
    16621 gtcgcgggtc atttcgagtg ctttcgcgtc gtttcgcgta gccagattca gcgtttccgc
    16681 agctcagcgc gggggttcga ttcccggcag ctccacagaa gggccgtaga gatgcggccc
    16741 ttcttctttg gcggtctgta tgaccaccgc gctgccgggt taagcaatag acgatctgcc
    16801 aagccagacc ctggaggtcg actatctgtc aagatgcaaa tctgtgtgat ccagcgcaaa
    16861 cacggtcatg aactggcttg ggccagttga tgccaaatcc tctggtctaa ggagcgatca
    16921 tgtcgcaccc cggcaggcat ccggttcagg ctgacaccca actcagctgc gcggctcacc
    16981 gacgcgcggc tttagttcgt cacaccggca tccaaggccg tagatcgtct aggtcacccg
    17041 gaggttctca aagaacgaaa gcaatgccga aatcagggac gctgctgtcc ttcggcctgc
    17101 tcgccgttgt tgggatcgcg gcgggtgatc ttatggccgc tggtcgggcc accgccgcgc
    17161 cgcaagaatt gatcttcacc gagcatgccg caaccgacac ggtcactgac accggcgcta
    17221 gcggagatac cgccggtgac gttcttacct tcgcaaacga ggtgtttgac gcagaagaca
    17281 gaaatcgcgt cggcagcgac cagggtatgt gtgtgcgtac cgtcccaggc aaggcgtggg
    17341 agtgcttctg gacgctgtcc ttggcatctg ggcagattac tgtcgatggt cccttctacg
    17401 actcaggtga ctcggtcctt gctatcaccg gcggcactgg agctttcggg aacgctcagg
    17461 gcgacatgct gttgaccccg atcggtacgg aaggtaacgc ttacaggttc acttacagac
    17521 tgctgtgagc cctgtcaccg ccgacatggt tcgttcattt ggctgacgga atgctcgtag
    17581 tggagggcag gtgagcccaa catcgacttc gaccctgacc gcccgactgg cgcgttggat
    17641 tccggcagtc ggatggctcg cgtcgtatcg gctgagttgg ctgcgggttg acctcgccgc
    17701 cggtatcaca gtcgcggcct acctgctccc ggccgggttg ggctacgcct cgctcgctga
    17761 cctgccgccc caagcggggc tctacgcgtg cctgtttggc gggctggtct tctggctgtt
    17821 ctgcagttcg cggcacacaa ccatctcggt cacctccgcc atctcgctgt tgatcggctc
    17881 gacactgggc accatctcgg gtggagactc gagccggttc accgcgctgg cggctggcac
    17941 ggccatcctc gtggggctca tcgccatcat cgcctggctg gtcaaggcgg gctcgatcat
    18001 caacttcatc tccgagagcg tcatgatcgg gttcaagtgg ggtgtggcgc tgttcctcgt
    18061 cagtacgcag ttgccgaagt tgtttggcac ccccgccgct cacggcgatt tctgggagaa
    18121 cagcggcacc ttcctgaagc acctgaacga gactaacctc gcagcgctga cgattggcgg
    18181 cacggcgctg gtggtgctcg tgctcgggaa gctcttggtt aagaagctgc caatgtcgtt
    18241 attggtcgtg gtggccggca tcgtggcttc gcgccgtctc ggactggacg agcgcggggt
    18301 gaagttgctc ggcgaagtgc cgcaaggctt gccggacctc ggccttcccg cgcttcattg
    18361 ggaagacctg aacgcaatgt tgcccctggc cctcgcgtgc ttcctgctcg ccgcggtgga
    18421 gacggccgcc attggccgca cgttcgcggc caagtacgac cggcggctgg acgccaacca
    18481 ggaattcctg gcgctcggcg cgtccaacct cgctgcgggg ttcggccaag gattcccggt
    18541 cagcggcggc atgtcgcaat ccgtcgtcaa cgagggcggg ggcgcgcgaa cgccgctgtc
    18601 gggcgccatt gcagcagtat tcattctggt ggtggtgctt ttcttctcgc acctgctgtc
    18661 cgcgttgccc caacccgtgt tggcggcggt cgtgttggtg gcggtggcca gcctggtcag
    18721 tctgtccgcc ctcaggcatc tctggcgcag tgatggccgc gaattcgtca tcgcgatggt
    18781 cgccatgttc ggggtgctgg ggcagggctt gctgcgtggc gtggcgatcg gcgcggtcat
    18841 ctcgctcgtg ctgttgattc ggcgcgcggc actaccgccc gtcgccttgc tcggccgcat
    18901 ccccggcacg cgccggtttt cggatcgcga gcgccatccg gagaacgaaa caattcccgg
    18961 catgctcata ttgcgcccgg gcgcgagctt gctttatttc aacatgaatt acgtgcgcga
    19021 cacgatagag gaacgtgtgc gggcggaaca ggaaaccaag ctagtcgtgc tggatctgtc
    19081 cgaagtgtcg atagtggaca tgcacgccgc ggagatgctg gcgggcctcg ctggcgaact
    19141 cgccgcgaag ggcatcaaga tccaagccgt ggaggcgcac tcgcaggtgc gcgaccggtt
    19201 gcgcagcgag ggactgaacg agagattcgg cgggatcgaa cggttgcgca ccgtcgccga
    19261 cgtggcggac gattttctgc gagggggtct cggccaggaa gatgtccacg tcgcgcttgg
    19321 tgatctaggc gctgtgccac ggccgggtcg gactgccaag ccagcagcag gcgctgacca
    19381 atctcgcgat cgtcccggcc gtcaaggctg gaaacgccgc ggcgataacg ggaagcgatg
    19441 agcgatctgg acgtgcggcc gagcctgctg agcgaagcgc tcgattaacc gctggaagcc
    19501 gcgtcgtgac tcacgtccgc agtacgatgc ggcgatgagt aagggtggga agaaatcggg
    19561 cgagaagtcg gcgcgaaaga tccccaaaaa cgtctaccaa gccgaacttt tccgccttca
    19621 aacggaattt gtgaggctcc aggaatgggt ccgggaaacc ggggcccgga tcgtgatcgt
    19681 cttcgaaggt cgcgacgccg cgggcaaggg tgggaccatc aagcgcatca ccgaatatct
    19741 gagccctcgt gtcgcgcgga tagccgcgct gccggcaccc accgagcggg agcgcgggca
    19801 gtggtactac cagcgctata tcgcgcacct gccggcgcgg ggtgagatcg tcctgttcga
    19861 caggtcctgg tacaaccgtg cgggtgtcga gaaggtgatg ggattctgta cgccgcaaga
    19921 gcattcgttg ttcctgaggc agacgcccat cttcgaacag atgctcatcg acgacggcat
    19981 catcctgcgc aagtactggt tctcggtatc cgacgaggag caactgcgac ggttcaggtc
    20041 ccggcgcaac gatccggttc ggcagtggaa gctctcgccg atggacctgg agtcgatctt
    20101 ccggtgggag gactactcgc gcgctaagga cgagatgatg gtgcacaccg acatcccgat
    20161 gagcccgtgg tacgtagtcg aatccgatag taagaagcac gctcgcctga acatgatggc
    20221 ccacctgctg tccacgatcg actacgaaga cgtcgaggct cccgcggtgg aattgccggc
    20281 gcacccggtg cggtccggca actattaccg accgccgcgt gagctgtcca cctatgtcga
    20341 cgactacgtg gccaccctgg tgggggatcc cgagtgaagc gcggtgttgg ctgtcgcccg
    20401 tgtgggtggt gtaggtgaaa cttcgcccgg cgcagtggtt cgggccgaac ggtactactt
    20461 gccaaagaag ccgatgtggt gacgcgcctc gttgcgatgg gtcgtgtgac tcaccggccg
    20521 ctcgagacaa aagatctcga taacgactgc ggcagaggcg actggggcgt gttgatgatg
    20581 tcaacacttt caatgcatcc gcaggtcgtt tcacgttgtt tcacgacgtc agattcggtg
    20641 ttttcttcaa catccgtggt taaggtcgcc aatcacggtc tcgtgcaacg ccatcggggt
    20701 gaccgctgtg cgcgcctctc gatgtagtcg atggcggcgt gtgcgcggtg ctccaggccg
    20761 aggcgcggtg gcggtgttgt ggagttcgtc aacgctattg aacgccaaat tttttcgcgc
    20821 ttccgcttct cggcgctcgg tctcgttgtc ggcgatgagg ccctcaagca gctgtcgatg
    20881 agcgatgccg cccctgccgc ggcgtctcgt gccagccgct tgtcgctggc gggcatggcg
    20941 ctgccggtgg tcagcgcgaa gcacgcgcac atcaacgacg gaggtgtggc ccgcgaccag
    21001 cgcctggcgg cgcccaccgt gggccagctg ctggcggcgg ccggtgcgcc gctccagcag
    21061 gacgacaagg tggtgccccc ggcgtggacg ccggtcatcg caggggccaa tagcttgagc
    21121 accgacgacg agaccgccac cgagcgcgac gcgcgcgttt tggtggtgcc gataacgctt
    21181 ctggaattca ccttcgatgc tgagcgctcg acgcggatcc ggccacgccc gaggtcgatg
    21241 tctcgcaccc gcagcgcggc cacctctccc caccggagcc ctgtcgtcgc gagcagcaaa
    21301 acgatgtcag gatgcttgga ctcgccggct aatgcggacg cctgcgtggc gggcgtttcc
    21361 cgcgcgaccg tataccggta cctcgcgcac gatgcggtcg cgtgaggttc gatccccgtc
    21421 agctccacga aacgggccga agctcagggg tcctttgctt tggcggttga cgatctattg
    21481 ccgcgccatc gcaagattga accgctcctc ggctgtgatc aggtggtcgt ggctgtcgtc
    21541 gccgatatcc agctggaccc attcgatctc acccgtgaag cggttgccgg tcgggccgta
    21601 gtccggcgac gcgggcgagc cggtgtcaga gcccacgtcg caggcttcgt cggcggaata
    21661 gcccatcggc tgggtctgct cgacgcggcc ggagcccacg gctttgccgt cgtagtacag
    21721 cgccacgtcg ccgcccttgg caagtccgcc gccgtcgtac ttgaactcca tccgcacctg
    21781 gtgtttccct ttcgggatcg gctttttcgc actcgtgatg tagtgctcga tcccgaagaa
    21841 gttgaagcag tacttgagtt ttccctcgtg tgcatagagc gaccagccgc cgacgctacc
    21901 gccctgggtg atgatcacac cgtcagcgcc ggattcgggt accacgatgt tggccgtcac
    21961 cgagtacgac ttgttcttca gtgtgagcac acagttttcg ctcactctca tcccgtcgaa
    22021 gagcaactga gtgttgccgc gaatcagctg cggcctcccg gcgatgtcgg ggttgacgcg
    22081 ttcaaatccc cggtcatcga gtggcacgac gttgtacttg accgcctcaa tcagccaaag
    22141 ccgctgcagc tcagccagtt tggcaggatt tctcttggcc agatcgtgcg cctgcgtcca
    22201 gtcatcgggt ccatagagct cccaggtgtc gtcatcgaag ggcgggggct ggtcggcctt
    22261 ccacggcgtc cggtgtttgg tcaccgccgt ccagccctgg tggtagatgc cacgattgcc
    22321 gaacatctca aagtattgga cttcgtgggt ctcgggtgct gcgccgtcgc gcagcatcgg
    22381 gagcatgctg acaccctcca gcggcgcctg cgcaataccg ttcacaaact ggggcgcggg
    22441 cagccccgcc gcttcgagga tcgtgggagc gacatcgacg acgtggtgga attggttgcg
    22501 ggtgttgccc ttctccgtca ggccgttcgg ccaatgcacg atcgcgccgt tgcgggtgcc
    22561 accccaatgc gacgccacct gcttagtcca ctgataaggc gcgcacagcg cgtgagccca
    22621 gccgaccgcg tagtggttgt aggccttcgg ggtgccgaag tcgtcgatct tggatagcag
    22681 aaactcagtg gtctcaatgc ccggcatgcc gttcaacgtc gtcatctcgt tgaagcagcc
    22741 gttcaatgtg ccctcagccg acgctccatt atcgccgatg atgtagtaga tcaacgtgtc
    22801 gcccagcact ccgagatcgt cgatggcatc gacgagccgc cccacttcat agtcggtctg
    22861 ctcgagaaag cctgcataga gttccatctg ccgcgcaaga actggcttga gttccgatgg
    22921 catgtcatcc cagccgggga tctcgtcgtg gcgcttggtc agttccgcgt ccttgggcac
    22981 cacacccagt ttcttctgct tgacgagcgt attgtcccgc agcgcatccc agccgtcgtc
    23041 gaacttgccc ttgtacttgt cggaccactc cttgggaacg tggtgggggg cgtgcgtggc
    23101 gcccggcgcg aagtacatga aaaacggctt atcgggcatc agtgccttct gctggcgcac
    23161 ccacgtgatc gcgcggttcg ccaggtcctc ggtgagcgtg tagccttcct cgggcgactt
    23221 gggaggctcg atcgccgtgg tgccttcgta taaacccggg tagtactggt tggcttcacc
    23281 gccgacaaac ccgtagaagt actcgaagcc cgaaccggtg ggccactggt ggaacgggcc
    23341 tgctggggag acctcccaga ccggtacctc gtgacacttg ccgaactgtc ccgtcgaata
    23401 gccgttgagc ttcaacgttt ccgcgatcgg cgccttctcc ttgggccgaa tgctgctgtt
    23461 ccccggagcg gaggtggcca tctccgtgat tgctcccatg cccactgagt ggtggttgcg
    23521 tccggtaagc atggcctggc gggtcggtga acacagagcg gtggtgtgaa agcggttgag
    23581 cttcagccca ttcgccgcca atcgctcagc gaccggcgta ttgcacggcc ccccgaacgc
    23641 cgatgacgcc ccgaaaccga catcatcgat gagcacaatc aatacgttcg gggcgcccgc
    23701 aggtggccgc agcatcgtga tcggtgggta cttcgtgttg ggatccttgg cgtcgtaagt
    23761 cgttagtccg acgtgctttg gatcgggaat cggtaagtgc tgccgcgata acggcgcagt
    23821 tgacggaacc tcggcgaccg gcgccggtgc ggccttcggg gctgctgact tcttcgtgct
    23881 tgccttcttg acgggctttg cggtcttgga tgtcttggcc attgtcgttc tcctcgagag
    23941 tgccggccga tacgatgctt attgccgtgc catggcaatt cgcgcgtgcg actgcttcac
    24001 ttcgtcctcg tcgtggtccg gcttcccgga cacgtcgacg gtcaccttgg ttatcctgcc
    24061 cgtgaacggg aagggtgacg cgtacatcac cgagacggag gatccgggat ttctgccgac
    24121 ggcgaaaccg ctacccaggc ccaacgcgag cggaacggtc accgggaaat ccgcctgcgc
    24181 gacgagttgg ctgtcgacga agagcttcgc gatgcccggt gtgcccttac cgttcttcag
    24241 gtcgggggcg ccggtcggtt cgaattcgta acgcaatgcg tgctttccgg gtggcaccgg
    24301 ttgatcggat gacacttgga gttcctccgc gccgacgtag ttgtgcacgt aatgcagttt
    24361 tccgtcttgt acgaagaaga cgtagccacc gacattgctg ccatggcaag ccaatacacc
    24421 ttcatcaccg gtcttcagct cggcgtcgac agtgattgtg tgcgcgcggt tgaggacctt
    24481 ggcggcgaca ttctcgggca ctgcctgggt gcgtgggaag taaacgtagg tgtcgcgctc
    24541 ggcggcaatc tgcggacgct catcggcgaa gcgcatcgtg ccgcggctgt cgagtggcag
    24601 cacgttgtac ttgccggcct ccgtgtacca gagcgcgatc atctcgatga gcttcgcgcg
    24661 ttcttggtcg gccaggttct tcgtctcggc cggatcgacc tcgacgttgt agagttccca
    24721 gcccgtggca tccagctcac gcagcttgtc ttcgctgagc tcaagcatgc cgaagctcac
    24781 cgccgcctcg gcgaaagacg ttccgggaaa ggggcatacc gcgcgccagc catcgtgata
    24841 gatcgagcgg tgcgcgaaca tctcgaagta ctgggtgtgg tggttggtcg gcgcacctgc
    24901 gtcgtcgaat gagtgcgcga agctcacgcc ttcgatcggg gattgcgcga cgccgcgcag
    24961 ctcactcggc gcctcgatgc ccagcgactc cagcacggtg ggcaccatgt cgatcgcgtg
    25021 ggcgtactga ttgcggatct cgcccttggc tttgatgccg ttgggccaat gcacgaggaa
    25081 tgggtcggag acgccgccgc gataggtctc gcgcttccac cgccgaaacg gagtgttgcc
    25141 cgcgaaagtc cagccccacg ggtagtggtt gaagtatttc ggcccgccga gttcgtcgag
    25201 cgccgcgaga ttctgttgca ggtcgtcggg cacgttgttg aagaacttgt tctcgttgac
    25261 cgagccgtgc ggcccgccct ccgcacttgc cccgttatcc gagatgacca tgaccagagt
    25321 gttttccagc tgtccgatcg cctcgagaaa gtccaggagg cgcccgatct ggtgatcggt
    25381 gtgctcgaag aatccggcga agacctccat catgcgtgcg tacagccggc gctcgtcggc
    25441 ggacaattcg tcccattgct gcacgtcggg atcgtgccgc gacagcacgg cgtccttggc
    25501 gacgatcccc agttcctttt ggcgcgcgaa caccttttcg cggtaggcat cccagccgtc
    25561 gtcgaacttg ccgtggtact tgtcggccca ttccttgggg acctggtggg gcgcatggtt
    25621 ggcgcccgtc gcgaagtaga ggaggaacgg tttgtcgggc gcgacctgct tgaggtcggc
    25681 aatgaactcg atggccttgt cggccagatc gacggtgagg tggtaaccct cctcgggggt
    25741 cttcggcggc tcgacttgat ggttgtcgaa gacaaggtcg ggatagtact gatgggtgtc
    25801 gccaccgagg aagccgtagt accggtcgaa gccgcgaccc agcggccagc gatcgtaagg
    25861 gccggccgcg ctgatctgtt ccgcgggggt caggtgccat ttgccgacgc agaaggtggc
    25921 atagccatgg ggtagcagca tctcggagag aaaaccgttc tcgaacggaa tcgcaccgtt
    25981 cgaacccggg aagcctgtgg acccctcggt gatgcacgcc ataccgttgg agtggtggtt
    26041 tcgcccgttg agcatgcatg accgcgacgg cgagcaaagc gccgtggtgt gcatgttcgt
    26101 gtactgaagc ccgttccgcg cgagccggtc caggttgggg gtattgatcg gcgaaccgta
    26161 gcaaccgaac tggccatagc cggtgtcgtc caacacgatg tacagaacgt ttggtgcgcc
    26221 gggcttcgag cgcaccggtg caggccacgc cggcgacgac tcgccgaccg ttcggcccat
    26281 ccggccgggg aacgcactgc ctggtttgta ctccgtcaag ctcatctcgt tctccatcgc
    26341 ttctgaggat ccttcgacgc actcgcgctt acgctgccgt aaacaagccc ggggtacgtc
    26401 aaaacggcca aaaaggcgcc gtgccaacgc aaaacacgag cgttgatggt cgcacctacc
    26461 gacggcatgg ttcagatcta atggtcggcc caagtggcag cgtggagctc atcgcgggct
    26521 tccgctatgt agacggcaac gtttgtaggt gggcaaaccg tcgtggccgc ctggtaaagc
    26581 cgcgacgtcg ggcccgggac gttgaaggtg gtgacgttca tcgacgggcg aagccgggct
    26641 ccttggccgg cgagacgcgt gttaatggtt ggcccgatgg tcagcatgct gaaggtcgga
    26701 gatgtctact cgtgactata gacagcgatc gggggttccg gtctggtgag ggctcgcgac
    26761 tgaagatccc gttggtactg ccgcggtgtc ttcccgaacc agcgtcggca cgagtgattg
    26821 agagtgctct gttccgtgta gccgagcaag cccgcgatct ggttgagatg cagctgaggt
    26881 tcggcgagga atctcgccgc cagggtccgc cgttcgcgtt cgaccaggtc ctgacatcgc
    26941 acgccctcgg tggccagccg tcgttgcagc gtccgtggat gtatcgcgag ttggtctgcg
    27001 atggcatcaa cgctgcattg gccggtcggc aggagccggc gggttagctc tgccacgcgg
    27061 tcggacagcg acgcggtgcg cggaaggtag ttggactcaa ggtatttcgc cgcaatccgc
    27121 ctggtctccg gatctgcgct ctcgatgcgt cggtcggcca agcggtgggg tagctcgaac
    27181 ccacaccagg tctggcggaa gcgcacgcgg caacccagcg tttcgcggta cgccgcgtcg
    27241 gggccctgct ggtcgtgcat gaacgagatc acgttcggcc gcgcctccgg gccgcccagc
    27301 aggcggatta tccgtacagc gatggcca
//
LOCUS       DASRJA010000430         9092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1956538,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000430 DASRJA010000000
VERSION     DASRJA010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9092
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956538"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1718
                     /gene="hutU"
                     /locus_tag="VFC01_18910"
     CDS             48..1718
                     /gene="hutU"
                     /locus_tag="VFC01_18910"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZN81730.1"
                     /translation="MNDPRHNIRDIFPPTGPEISAKSWLTEAPMRMLMNNLHPDVAEN
                     PHELVVYGGIGRAARTWADFDRIVATLKTLGDDETLLVQSGKPVGVFRTHEWAPRVLI
                     ANSNLVGDWANWEQFRKLEAEGLMMYGQMTAGSWIYIGTQGILQGTYETFGAVAKKRF
                     GGTLAGTITLTAGLGGMGGAQPLAVTMNDGVAICIDCDETRIDRRIEHRYLDTKAVDI
                     DDALRLAVEARDAKRPLSIGLLGNAAEVLPELLRRNAPIDIVTDQTSAHDPLSYLPIG
                     IDFDDMKKMADKDPVYFTEQAQQSMAKHVAAMVGFQDRGAEVFDYGNSIRDEARKAGY
                     DRAFDFPGFVPAYIRPQFEEGLGPFRWAALSGDPKDIAATDRAILELFPENKHLTRWI
                     EMAGEMVAFQGLPARICWLGYGERHRAGLKFNEMVASGELSAPIVIGRDHLDSGSVAS
                     PYRETEAMLDGSDAIADWPLLNALVNTASGATWVSIHHGGGVGMGRSIHAGQVTVADG
                     TELAAQKLARVLTNDPGMGVIRHVDAGYEHATAVAEQRGVRIPMQENV"
     gene            1715..2941
                     /locus_tag="VFC01_18915"
     CDS             1715..2941
                     /locus_tag="VFC01_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HZN81731.1"
                     /translation="MTAFDALWPTILDVGRHASTGGYRRYAWSDADLTLREWFTGCAA
                     ERSMDVEEDRNGNLWAWWMPPGWSGDPTDAFVTGSHLDSVPDGGAFDGPLGVVSAFAA
                     VDIVRERGVVPRVPVAVVAFSDEEGARFGVACVGSQLSTGAVSAEKARGLRDVHGVTL
                     DEAMTKAGRHPEHLGADPRLADRVGVFVELHVEQGRALDLVDRPIAVASAIWPHGRWQ
                     FTFCGEANHAGTTRLADRHDPMLAFASSVHAARSLAATHDAVATFGKVIVAPNGANAI
                     PSSVQAWLDARAADEATLRSLVEKITTEARQHAAADGIELDVIAESVTPIVEFPHTPR
                     ARLQRALAHLGDIPVLPTQAGHDAGILSAVVPTAMLFVRNPTGVSHSPVEFAEASDCN
                     TGAVALADVMADWVAP"
     gene            2938..4287
                     /locus_tag="VFC01_18920"
     CDS             2938..4287
                     /locus_tag="VFC01_18920"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HZN81732.1"
                     /translation="MTDTWWCEHAWLGESVADGVLFTVADGRITAIDTDTSAQPGSHR
                     LRGLVVPGLANAHSHAFHRALRARTQRDRGSFWTWRDLMYRAAERLNPERYRRLTRAV
                     YAEMTLAGITAVGEFHYVHHDADGSRYTDPNAMGQAVIGGAADAGLRLTLLDTCYLSS
                     GPDGAPLAGPQLRFGDDTGEQWAERVEALRQQVGAHSSVLVGAALHSVRGVPVEHMLA
                     VVKWAEGHGVPLHIHSSEQTREVDECLAVHGRTPTAVLRDVGALGPHTTAVHATHLTA
                     EDIADLDRTSTGVCFCPTTERDLGDGIGPAPALLAGSGLFSLGSDSHAVIDLFEEARA
                     VELNERLASHTRGIVAADRLLRAATFDGHRALGWSDAGELKVSARADFVAVRTDSIRT
                     AGGGATVENVVFAAAAPDVTDVVVDGRAVVIDRKHLTVPDAEAELVSAIADLMEASR"
     gene            4284..5483
                     /gene="hutI"
                     /locus_tag="VFC01_18925"
     CDS             4284..5483
                     /gene="hutI"
                     /locus_tag="VFC01_18925"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HZN81733.1"
                     /translation="MTVLYTDIRELVTNVPANADGDPLGIIEDAALVVDGDQIAWIGK
                     RADAPDADERISCADTSVIPGFVDSHTHLVFAGERSAEFAARMSGQSYEAGGIASTVT
                     ATRKADDATLLAGVQRLADELLRAGVTTFECKSGYGLTVDDEARSLQIAKAVTDETTF
                     LGAHVVPAEYRDDRAAYIDMVTGPMLQACAPHARWVDVFCDRGAFDVDESRHILTAGI
                     AAGLDARLHAGQLGPSAGIRLAVELGAASVDHCTYATDEDVDALASAADTTVATLLPG
                     AEFSTRARYPDGRRLIDAGATVALATDCNPGSSFTTSMPFCIAVAVRDMNFTPAQALW
                     AATAGGAAALRRDDVGVLAVGRRADFVRLDAPSYLHLAYRPGVPLVRDVVKDGHHMTN
                     EKVESRS"
     gene            5477..7024
                     /gene="hutH"
                     /locus_tag="VFC01_18930"
     CDS             5477..7024
                     /gene="hutH"
                     /locus_tag="VFC01_18930"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005572535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HZN81734.1"
                     /translation="MVSTETVVVGVGPVSFDEVVRVAREGATVALSEESLDAIAKSRA
                     RIEQLAHDPKPVYGVSTGFGALATRHIPHELRTQLQRSLIRSHAAGSGPEVEREVIRA
                     MMLLRLSTLATGRTGVRPEVAQTYAAMLSVGLTPVVYEYGSLGCSGDLAPLAHVALAA
                     IGDGFVRTADGERLPSADALARHGIAPVVLAEKEGLALINGTDGMLGMLVLALRDLDR
                     LVKLADVAAAMSVEGLLGTGRVFAEDLHAMRPHPGQAAAAANMRRVLADSPIVASHSG
                     PDCSLVQDAYSLRCAPQVAGAVRDTVDHARTIAGRELASAIDNPVVTLDGRVESNGNF
                     HGAPVGYVLDFLAIAVADLASISERRTDRFLDVARSHGLNPFLADDPGVDSGHMIAQY
                     TQAAIVSELKRLANPASVDSIPSSAMQEDHVSMGWSAARKLRRAIDGLTRVLAIETLT
                     AARGIEMRAPLAAAPATAAVIAAIREAVAGPGTDRYLSPEIDAVVDLAASGILVAAAE
                     SVTGPLA"
     gene            complement(7035..7298)
                     /locus_tag="VFC01_18935"
     CDS             complement(7035..7298)
                     /locus_tag="VFC01_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81735.1"
                     /translation="MNVHVVVGRRCPFCQHTVNTFVDPRDPANTPHDGDISICSNCSG
                     LSVFQGNELRLPTPEELARSMRDKKLLHSIKELRAEQYGWSVF"
     gene            complement(7484..8074)
                     /locus_tag="VFC01_18940"
     CDS             complement(7484..8074)
                     /locus_tag="VFC01_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN81736.1"
                     /translation="MTTVRARAGTPRAGTPLGRAEVVAAVLESAADLFAERGPAATSI
                     RDIASRSRVNHGLIHRHFGNKDRLVGAVLDYLGQHLAELLGAHADGRVIGDAVDRQLR
                     VLARTSLDGYSIGELQSRFPTMEQLLETVRARHSSELGARLAAAHTIALQLGWCLFGD
                     FLRASTGLEDVDDRTFARSVGNTVALILDAEGPAPS"
     gene            8137..>9092
                     /locus_tag="VFC01_18945"
     CDS             8137..>9092
                     /locus_tag="VFC01_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81737.1"
                     /translation="MATTPYSYGRFIGRVGGLAVALGIGAAIANNSAVAAAEDGQSSP
                     SEASSSAGSESSAGPTAKSPDSPEPQPSNSAASTTNDDAAESDTPTDGHTTPDQKADT
                     SSPKSTVSAQTVTLHDDTKATTGKDPAPKPAAPAETLAMASTLATANRERAQAFASDP
                     TDTTTQPVAAAQTVAVSPLGTPEQLEAERRAAETVNTLPVQFMKLVLQFGWRATAQQQ
                     FNLVGGPDQENLDALNKAVDEYAMGAAFQQQLLNPMTPTAVMQVAPPHIWYGQDVSGS
                     RILYDNPDTIYRFMAVNKTSTYVITGRFTGEMPADTTFSVLTG"
BASE COUNT         1526 a         3155 c         3002 g         1409 t
ORIGIN      
        1 accatcattt ccgcacagct tctggcctga gcccagggag gaaagacatg aacgaccccc
       61 gccacaacat ccgcgacatc ttcccgccga ccggccccga gatcagcgcc aagagctggc
      121 tgaccgaggc gccgatgcgg atgctgatga acaacctcca ccccgacgtc gccgagaacc
      181 cgcatgagct cgtggtctac ggcggcatcg gccgggcggc gcgcacctgg gccgacttcg
      241 accgcatcgt cgccaccctg aagaccctcg gcgacgacga gaccctgctg gtgcagtccg
      301 gcaagcccgt gggcgtgttc cgcacccacg agtgggcgcc gcgcgttctg atcgccaact
      361 cgaatctcgt tggcgactgg gcgaattggg agcagttccg caagctcgag gccgagggcc
      421 tgatgatgta cgggcagatg acggcgggct cgtggatcta catcggcacc cagggcatcc
      481 tgcagggcac ctacgaaacc ttcggcgcag tcgcgaagaa gcggttcggc ggcacactcg
      541 cgggcacgat caccctgacc gcgggactcg gcggcatggg tggggcgcaa ccactggccg
      601 tcaccatgaa tgacggcgtg gcgatctgca tcgactgcga cgagacccgc atcgaccggc
      661 gtatcgagca ccgctacctc gacaccaagg ccgtcgacat cgacgacgcg ctgcggctcg
      721 ccgtcgaggc ccgcgacgcc aagcggccgc tgtccatcgg tctgctgggc aatgccgccg
      781 aggtacttcc cgagctgctg cgccgcaacg cgccgatcga catcgtcacc gatcagacct
      841 ccgctcacga cccactgtcg tacctgccga tcggcatcga cttcgacgac atgaagaaga
      901 tggccgacaa ggatcccgtg tacttcaccg agcaggccca gcagtcgatg gccaagcacg
      961 tcgcggcgat ggtcggattc caagaccgag gagccgaggt gttcgactac ggcaactcga
     1021 tccgcgacga ggcccgaaag gccggatacg acagggcttt cgacttcccc ggcttcgtcc
     1081 ccgcgtacat caggccgcag ttcgaagagg gccttgggcc attccggtgg gccgcgttgt
     1141 cgggtgatcc gaaggacatc gccgccaccg atcgggcgat cctcgaactg ttcccggaga
     1201 acaagcacct cacccgctgg atcgagatgg ccggcgagat ggtcgcattc caggggctgc
     1261 ccgcacgcat ttgctggctc ggctacggcg agcgtcatcg ggccgggctg aagttcaatg
     1321 agatggtggc gtcgggcgaa ctgtccgcgc cgatcgtgat cggccgcgac cacctggatt
     1381 ccggctcggt cgcctcgcca tatcgggaga ccgaggcgat gctcgacggt tccgacgcga
     1441 tcgccgactg gccgctgctc aacgcgctgg tcaacaccgc atccggcgcg acgtgggtgt
     1501 ccattcacca cggcggcggt gtcggcatgg gccgctccat tcacgccggc caggtgaccg
     1561 tcgctgacgg caccgaactg gccgcgcaga agctcgcgcg ggtgctgacc aacgatccag
     1621 gcatgggcgt gatccgtcac gtcgacgccg gttacgaaca cgcgactgcg gtcgccgaac
     1681 aacgcggcgt gcgaattccc atgcaggaga acgtgtgacc gcgttcgacg cgttgtggcc
     1741 gacaatcctg gacgtcggca ggcacgcctc caccgggggc tatcgccgct atgcgtggtc
     1801 ggacgccgat ctgacattgc gcgagtggtt caccggctgc gccgccgagc ggagcatgga
     1861 tgtcgaagag gaccgcaacg gaaacctttg ggcgtggtgg atgccgccgg gctggtccgg
     1921 agatccgacc gacgcgttcg tcacgggttc gcacctggat tcggtacccg acggtggcgc
     1981 gttcgacggg ccgctcggtg tggtgtccgc cttcgccgcc gttgacattg tccgcgagcg
     2041 cggcgtggtg ccacgggtgc ccgttgctgt ggtcgccttc tccgacgagg agggcgctcg
     2101 attcggcgtt gcctgcgtgg gctcgcagct gtctaccgga gcagtgagcg ccgagaaggc
     2161 ccgcgggctg cgggacgtcc acggagtgac gttggatgag gcgatgacga aggccggccg
     2221 ccatcccgag catctcggcg ccgacccgcg gctggccgat cgcgtcggcg ttttcgtcga
     2281 actgcacgtc gagcagggcc gcgcgctgga cctggtcgac cggccgatcg cggtcgcatc
     2341 cgctatctgg ccgcacggcc ggtggcaatt cacgttctgc ggcgaggcga accacgccgg
     2401 caccacgcgg ctggccgacc ggcacgaccc gatgctcgcg ttcgcgtcct cggtgcatgc
     2461 cgcgcggtcg ttggccgcca cccacgatgc ggtcgcgacg ttcggcaagg tcatcgttgc
     2521 gcccaacggc gccaacgcga ttccgtcgtc cgtgcaggcc tggctcgatg ccagagcagc
     2581 cgacgaagcg acgttacggt cactcgtcga gaagatcacc accgaagcgc ggcagcacgc
     2641 cgcggccgat ggcatcgaac tcgacgtcat cgccgaatcg gtcacgccga tcgtcgagtt
     2701 tccgcacacc ccacgggctc ggctgcaacg tgcgctcgcc cacctgggcg acatcccggt
     2761 gctgccgacc caggccggcc acgacgccgg catcctgtcc gcggtggtgc cgaccgcaat
     2821 gttgttcgta cgcaacccaa ccggcgtgtc gcactccccc gtggaattcg ccgaagcatc
     2881 cgactgcaat acgggagctg tggcgctggc cgacgtgatg gcagattggg tggcaccgtg
     2941 acggacacgt ggtggtgcga acacgcctgg ctcggcgagt cggtcgccga cggcgtgctg
     3001 ttcaccgtcg ccgacggccg catcactgcg atcgacaccg acacctccgc gcagccaggc
     3061 tcacaccggt tgcgcgggct cgtcgtcccg ggactggcca acgcccattc gcacgctttc
     3121 caccgagccc tgcgcgcccg cacccaacgc gaccgtggtt cgttctggac ctggcgcgac
     3181 ctgatgtacc gcgccgccga gcgactgaac cccgagcgct accgccggct gacccgcgcg
     3241 gtgtatgccg aaatgacact cgccggaatc actgcggtcg gcgaattcca ctatgtacac
     3301 cacgacgccg acggctcccg ctacaccgat ccgaatgcca tgggacaggc cgtgatcggc
     3361 ggcgcggccg acgccgggct ccgccttacc ctgctcgaca cctgctatct gagttccggg
     3421 ccggacggcg caccgctcgc cggcccacag cttcggttcg gcgacgacac cggtgagcag
     3481 tgggcggaac gcgtcgaggc gctacggcag caggtcggcg cgcactcatc agtgctcgtc
     3541 ggggccgcgc tgcattccgt ccgtggcgtc cccgttgagc acatgcttgc ggtcgtgaaa
     3601 tgggctgagg gacacggtgt tccgctgcac atccactcct ccgagcagac ccgcgaggtc
     3661 gacgagtgcc tggccgtgca cggccgcacg ccgaccgcgg tgctacgtga cgtcggggcg
     3721 ttggggcccc acacgacggc cgtgcacgcc acccacctca ccgccgagga catcgccgac
     3781 ctcgaccgga cgtcgaccgg agtgtgcttc tgccccacca ccgagcgcga cctcggcgac
     3841 ggaatcggac ccgcgcccgc gctgctcgca gggagcggtc tgttcagcct cggctccgac
     3901 agccacgccg tgatcgatct gttcgaggag gcccgcgccg tcgaactcaa cgaacgcctc
     3961 gcctcccaca cgcgcggcat cgttgccgcc gaccgcctgc tgcgggccgc cacctttgac
     4021 gggcatcgcg ccctcggctg gtcggacgcc ggcgaactga aggtcagcgc acgagcggat
     4081 ttcgtcgcgg tccgaaccga ttcgatccgc accgcgggcg gcggtgcgac ggtggaaaat
     4141 gtcgtctttg ccgcggcggc acccgatgtc accgacgtgg tggtcgacgg ccgcgccgtg
     4201 gtgattgacc gcaagcatct cacggtgccc gacgcggaag ctgaactcgt ttcggccatt
     4261 gcggatctga tggaggcgtc ccgatgaccg tgctttacac cgacatccgc gagcttgtca
     4321 ccaacgtgcc cgccaacgcc gatggcgacc cgctcgggat catcgaagac gcggcactgg
     4381 tggtcgacgg cgaccagatc gcctggatcg gcaagcgcgc cgacgcaccc gatgccgacg
     4441 aacgaatcag ctgcgccgac accagcgtga tccccggatt cgtcgacagc catacccatt
     4501 tggtcttcgc gggcgagcgg tcggcggaat ttgcggcgcg gatgagcggc caatcgtacg
     4561 aagcgggcgg gatcgcatcg acggtgaccg cgacacgtaa ggccgacgat gcgacgcttc
     4621 tggccggcgt ccagcgcctc gccgacgagt tgctgcgcgc cggcgtgacc accttcgagt
     4681 gcaagagcgg ctacgggcta acggtcgacg acgaggcccg ttcgttgcag atcgccaagg
     4741 ccgtcaccga tgagacgact ttccttggcg cgcacgtcgt tccagccgag tatcgcgacg
     4801 accgcgcagc atacatcgac atggtcaccg gtccgatgct gcaagcctgc gcaccgcacg
     4861 cgcggtgggt ggacgtgttc tgcgaccgcg gtgccttcga tgtcgacgag tcccgtcaca
     4921 tcctgaccgc cggaatcgcc gccgggctcg acgcgcggct gcatgccggc caactcggac
     4981 ccagcgcggg catccggctc gccgtcgagc tgggcgcggc gtcggtcgat cactgcacct
     5041 atgccaccga cgaggatgtc gacgcgctcg cctcggccgc cgataccacc gtcgccacgc
     5101 tgttaccggg cgccgaattc tcgacgcgcg cacgatatcc ggatggccgg cgactgatcg
     5161 acgcgggcgc gacggtggcg ctggcgaccg actgcaatcc gggcagctcg ttcacgacaa
     5221 gcatgccgtt ctgcatcgcc gtcgcggtgc gcgatatgaa tttcactccc gcccaggcgt
     5281 tgtgggccgc gaccgcgggc ggggcggcgg cgctgcgccg cgacgacgtc ggggtccttg
     5341 ccgtcggccg gcgggcggac ttcgtccggc tggacgctcc gtcgtacctc cacctcgcgt
     5401 accgaccagg cgtgccgctg gtgcgcgatg tcgtcaagga cggccaccac atgaccaacg
     5461 agaaagtgga gtcacgatcg tgagtaccga aaccgttgta gtgggcgtcg gccccgttag
     5521 cttcgacgag gtggtgcgcg tcgcccgcga gggcgccacg gtcgccctgt ccgaagagtc
     5581 actggacgcg atcgccaaga gccgcgcgcg catcgagcag ctggcccatg accccaagcc
     5641 cgtctacggg gtatccaccg ggttcggcgc actcgccacc cggcacatcc cccacgagct
     5701 gcgaacccag ttgcagcgca gcctgattcg ctcccacgcc gccggatccg gtcccgaggt
     5761 cgaacgggaa gtcatcaggg ccatgatgct gctgcggttg tcgacgctgg ccacgggccg
     5821 caccggggtg cggcctgagg tcgcgcagac atacgcggcg atgctgtctg tcggactgac
     5881 gcccgtcgtc tatgagtacg gcagccttgg ctgctccggg gacctcgcgc cgctggccca
     5941 cgtcgcgctg gccgcaattg gcgacgggtt cgtgcgcacg gccgacggcg aacggctgcc
     6001 ctccgccgac gcgctggccc ggcatggcat cgcgccggtg gtgctcgctg agaaggaggg
     6061 gctggcgctg atcaacggca ccgacggcat gctcggcatg ctggtgctgg cgctgcgcga
     6121 cctcgaccgg ctggtgaagc tcgccgacgt cgcggcggcc atgagcgtcg agggattgct
     6181 cggcaccggc cgcgtgttcg cggaggatct gcacgcgatg cgcccgcatc cggggcaggc
     6241 cgccgcggcc gccaacatgc gccgcgtgct cgccgactcg ccgatcgtcg cgagccacag
     6301 cggcccggac tgctcccttg tccaggacgc gtactcactg cgctgcgcac cccaggtcgc
     6361 cggcgcggtc cgcgacaccg tcgaccatgc ccgcacgatc gccggccgcg aattggccag
     6421 tgccatcgac aatcccgtcg tcacgctcga cggccgggtc gaatcgaacg gcaacttcca
     6481 cggcgccccg gtcggctatg tattggactt cctcgccatc gctgtcgccg atctggccag
     6541 catcagcgag cggcgcaccg accggttcct cgatgtcgct cgcagccacg gcctcaaccc
     6601 atttttggcc gacgatcccg gtgtggacag cgggcacatg atcgcccagt acacgcaggc
     6661 cgcaatcgtc tccgagctca aacggctggc caacccggcc agcgtcgact ccatcccgtc
     6721 atcggcgatg caggaggacc acgtatccat ggggtggtca gcggcgcgca agctgcgcag
     6781 ggccatcgac gggctcaccc gcgtcctggc gatcgagaca ctcaccgccg cacgcggtat
     6841 cgaaatgcgc gccccgctgg cggcggcacc cgccacggcg gccgtcatcg ccgcgatccg
     6901 ggaagccgtc gcggggcccg gcaccgaccg ctatctgtcg cccgaaatcg atgccgtcgt
     6961 cgacctcgct gcctcaggga tactcgtggc agccgcggaa tcagtcaccg gtccgttggc
     7021 ctgaagccag cggttcagaa cacgctccac ccgtactgct cggcccgaag ctccttgatc
     7081 gagtggagca gttttttgtc gcgcatggac cgcgccagtt cttcgggtgt cggcagccgg
     7141 agttcattcc cctggaagac cgacaatccg ctgcaattgg agcagatcga gatgtcgcca
     7201 tcgtgcggtg tgttggcggg atctcgtggg tcgacgaagg tattgaccgt gtgctgacag
     7261 aacggacacc gtcgccccac cacgacgtgg acattcatct tgtttacgtt aggcccgatg
     7321 caggcgtctg caaatgctta tggcgaacgt acttggattc ttgttgcaag cgaaacgacg
     7381 cgattggatg gccaaaatcg actggggcaa agtcgatttc gcgcgcgtaa acgcttcgaa
     7441 ctgcacggtg ggtggtgagt tgtccactta gcgaaattct tggctacgac ggtgccggac
     7501 cctcggcgtc gagaatcaag gcgaccgtgt tgccgaccga gcgggcgaac gtgcgatcgt
     7561 cgacgtcctc cagaccggtc gatgcgcgca ggaagtcgcc gaacaggcac cagccgagct
     7621 gcagcgcgat cgtgtgcgcg gcggccagcc gcgcgcccag ttcgctggag tgccgcgcac
     7681 gcacggtctc caggagctgc tccattgtcg gaaagcggct ctgaagctcg ccgatcgaat
     7741 acccgtcgag tgaggtacgg gcgagcacgc ggagctgacg gtcgaccgcg tcgccgatga
     7801 cgcgtccgtc cgcgtgtgcg ccgagaagtt cggcgagatg ctggccgaga tagtcgagca
     7861 ccgcaccaac gagccggtcc ttgttgccga agtgccggtg gatcagaccg tgattgaccc
     7921 gcgagcgcga tgcgatgtcg cggatggagg ttgctgcggg tccgcgttcg gcgaataggt
     7981 ctgcggcaga ctccagcacc gccgcgacga cttccgccct gcccaggggc gtaccggcgc
     8041 gtggggtgcc ggctcttgca cgaactgtag tcacatgact acggtaccgg gcaaggcgtt
     8101 tgcggtcagc agacgcctcg gcggaaggaa tcgcccgtgg caacgacgcc atactcctac
     8161 ggtcgtttta tcggcagggt cggcggcttg gcggtcgcac tggggatcgg cgcggcgatt
     8221 gcgaacaact ccgcggtcgc cgccgctgag gacggtcagt cgagtccctc agaggcatcg
     8281 tcatcggcgg gctcggaatc gtcggcgggg cccaccgcga agtcgccaga ctcgccggag
     8341 cctcagccct cgaattccgc ggcgtcaaca acaaatgatg acgccgccga atcggatacc
     8401 ccaacggacg ggcacacaac cccggaccag aaggccgaca cgtcgtcgcc gaagtcaacg
     8461 gtgagcgcgc agaccgtcac cctgcacgat gacaccaagg caaccaccgg caaagatcca
     8521 gcgccgaagc cggccgctcc cgccgagacc ctggcgatgg cctcgacgct ggcaacggcc
     8581 aaccgcgaac gcgcacaagc ctttgcctcc gaccccaccg acaccaccac acagccggtg
     8641 gcggcggccc agacggtggc ggtcagtccc ctgggaacgc cggaacaact cgaagccgag
     8701 cgacgcgccg ccgaaacggt caacacgctt ccggttcagt ttatgaagct cgtgctccaa
     8761 ttcggttggc gcgcaaccgc acaacagcag ttcaacctgg tcggcggccc ggaccaggag
     8821 aatcttgacg cgctcaacaa ggccgtcgac gagtacgcca tgggggccgc attccagcag
     8881 caactgctca atcccatgac gccgacggcc gtcatgcagg tggccccgcc gcacatctgg
     8941 tacgggcagg acgtgagcgg ctcgcgcatc ctctacgaca acccggacac catctaccgc
     9001 ttcatggcgg tcaacaagac ctcgacgtat gtgatcacgg gccgcttcac cggcgagatg
     9061 ccggccgaca cgacgttcag cgtgctgacc gg
//
LOCUS       DASRJA010000431         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3668422,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000431 DASRJA010000000
VERSION     DASRJA010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3668422"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFC01_18950"
     CDS             complement(<1..378)
                     /locus_tag="VFC01_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HZN81738.1"
                     /translation="MRVAAPVSLPSYTVAVMEQPGELVGRALVVVVDDRTAHGEEDHS
                     GPLVTELLGEAGFVVDGVVVVSADEVEIRNALNTAVIGGVDLVVSVGGTGVTPRDVTP
                     EATRDILDRELLGISEALRASGLS"
     gene            complement(402..1757)
                     /locus_tag="VFC01_18955"
     CDS             complement(402..1757)
                     /locus_tag="VFC01_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN81739.1"
                     /translation="MTNHPRYSPPPPQPGHRPGGHEQHAAPGYPGAYRPGPYQQPQQQ
                     QPYDWRYATQQQFRTPYDPYRGAPQPSGVPSMPPVRQKRSRAGALTAGAVAVAIVSAG
                     IGGGVALLVQPDRPSAGSSVTGLAPSMPAASLPAGSVEQVAAKVVPSVVKLEVDLGRQ
                     SEEGSGIILSSDGLILTNNHVVAAAKDSSAGPAAAGGAQTKVTFADGHTTGFTVVGTD
                     PSSDIAVVRVQGASGLTPITLGSSGNLRVGQDVVAIGSPLGLEGTVTTGIISALNRPV
                     AAGGDAQNQNTVLDAIQTDAAINPGNSGGALVNMNGELIGVNSAIATLGADAGPAAQS
                     GSIGLGFAIPVDQAKRIADELIKSGTASHASLGVQVGNDAAVDGAKIVEVTSGGAAAA
                     AGLPSGVVVTKVDDRVIGSADALVAAVRSKAPGDKVTLTYLDPAGKPQTVQVTLGKAQ
                     Q"
     gene            complement(1905..3377)
                     /locus_tag="VFC01_18960"
     CDS             complement(1905..3377)
                     /locus_tag="VFC01_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN81740.1"
                     /translation="MSAQKFGFRPQARRPPLTAASSVSLRWRVMLLAMSMVAMVVVLI
                     SVAVYAVVSRALYDDLDNQLHSRARLLIESGSLAADPGKAIEGTAYSDVNAMLVNPSR
                     YIYTANQEGQTLPLGEPEKAVVSGELLMSLRTANHQRVLALHLTNGSSLLISKSLAPT
                     SQVLKRLGTVLLIVGGLGVVVAAMAGGAVARAGLRPVARLTEAAERVARTDDLRPIPV
                     LGSDELARLTEAFNMMLRALAESRERQARLVTDAGHELRTPLTSLRTNVELLMAAMAP
                     GAPRLPDDEMAGLRADVIAQIEELSTLVGDLVDLTRDEAGGVIHEPVDMSDVVDRSLE
                     RVRRRRSDIEFDVSVTGWQVYGDGGGLARAVLNLLDNAAKWSPPGGRVGVRLTQIDPS
                     HAELVVSDFGPGIPPQERRLVFERFYRSTSARAMPGSGLGLAIVKQVVVKHGGTLRVE
                     DTVPGGQPPGTSIHVVLPGRPLPTAQPADMDDRVVSADNV"
     gene            complement(3377..4063)
                     /locus_tag="VFC01_18965"
     CDS             complement(3377..4063)
                     /locus_tag="VFC01_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN81741.1"
                     /translation="MRILVVDDDRAVRESLRRSLSFNGYSVELAQDGREALDVIANDR
                     PDALVLDVMMPRLDGLEVCRQLRSTGDDLPILVLTARDSVSERVAGLDAGADDYLPKP
                     FALEELLARMRALLRRRTPDDDAESPALTFSDLSLDPVTREVTRGDRQISLTRTEFAL
                     LEMLIANPRRVLTRSRILEEVWGFDFPTSGNALEVYVGYLRRKTEAEGEPRLIHTVRG
                     VGYVLRETPP"
     gene            complement(4131..4304)
                     /gene="rpmF"
                     /locus_tag="VFC01_18970"
     CDS             complement(4131..4304)
                     /gene="rpmF"
                     /locus_tag="VFC01_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZN81742.1"
                     /translation="MAVPKRRMSRANTRSRRAQWKAKRPELVNVTVAGQQHKVPRRLL
                     KAARLGLIDLDRR"
BASE COUNT          704 a         1509 c         1444 g          719 t
ORIGIN      
        1 cgataggccg gaggcgcgta gcgcctcgga gatgccgagc agttcacgat cgaggatgtc
       61 gcgggtcgct tcgggcgtga cgtcacgtgg cgtcacaccc gtgccgccca ccgacaccac
      121 caagtccact cccccgataa ctgcggtgtt gagggcattg cggatctcca cctcgtcggc
      181 agagaccacg actacgccgt ccaccacgaa tcccgcctca ccaagcaact cggtgaccaa
      241 cggaccgctg tggtcctcct cgccatgcgc ggtgcggtca tcgacgacga cgacgagcgc
      301 ccggcccacc aactcccctg gctgttccat gactgcaacc gtataactgg gaagcgacac
      361 cggcgcggcc actctcagtg gccgaggccc tgacgtgacg ctcactgctg cgccttgccg
      421 agggtgacct gcacggtctg cggcttgccc gccggatcga ggtacgtcag cgtgaccttg
      481 tcccctggcg ccttggatcg caccgcggcc accagtgcgt cggcgctgcc gatcacccgg
      541 tcgtccacct tggtcaccac gacgccgctg ggcagtcctg cggccgccgc ggcaccgccg
      601 ctggtgacct cgacgatctt cgcgccgtct acggcggcat cgttaccgac ctgcacgccg
      661 agcgaggcgt gcgatgcggt tccgctcttg atgagctcgt cggcgatccg cttggcctgg
      721 tccacgggga tcgcgaaacc cagcccgatg gagccgcttt gggccgcagg tccggcgtcc
      781 gcgcccaatg tcgcgatcgc cgaattcaca ccgatcaact cgccgttcat gttgaccagc
      841 gcgccgcccg agttacccgg gttgatcgcg gcgtcggtct ggatggcgtc aagcaccgtg
      901 ttctggttct gcgcgtcgcc gcccgcggcc accggccggt tcagcgcgct gatgatgccg
      961 gtggtcacgg tgccttcgag ccccagcggt gagccgatgg cgaccacgtc ctggcccacc
     1021 cgcaggttgc ccgacgagcc aagcgtgatc ggggtcaagc ccgacgcacc ctgcacgcgg
     1081 accaccgcga tgtcactgct ggggtcggtg ccaacgacgg tgaaacccgt tgtgtgaccg
     1141 tccgcgaagg tcacctttgt ctgggcgccg ccggcggcgg cgggcccggc tgagctgtct
     1201 ttggcggcgg ccacgacgtg gttgttggtc aggatcagcc catcggagga caggatgatg
     1261 ccggagccct cttcagactg tcgcccgagg tcgacctcca gcttgaccac gctgggcacc
     1321 accttggccg cgacctgctc gacggagccg gcgggcaggc tggcagcagg catgctgggc
     1381 gccagaccgg tcacgcttga gccggccgac ggccggtccg gctgcacaag cagcgcgact
     1441 ccgccgccga ttccagccga cacgatcgcc actgcgaccg cgccagctgt caaagctcct
     1501 gcacgagaac gcttttggcg cacgggcggc atcgagggca caccggatgg ctgcggggca
     1561 ccgcggtacg gatcgtaggg cgtacggaac tgctgctggg tggcgtatcg ccagtcgtat
     1621 ggctgctgct gctggggctg ctggtaggga ccgggccggt atgcaccggg gtatccgggc
     1681 gccgcgtgct gctcatggcc gccaggccgg tgacccggct gcggtggcgg cggcgagtac
     1741 ctcgggtggt tcgtcatgtg gctgcggtgc tcttcctgga gtaggtaccg acatctggaa
     1801 caacgatgga tgcaagctca gggtgcccat ttgggctgag aatccactta gagaactttc
     1861 gggagatgcc cagactttcc tcccccttca acgaactccc ccgatcacac attgtcggcg
     1921 ctgaccaccc gatcgtccat gtcggcgggc tgtgccgtcg gtagtggccg gccgggcagc
     1981 accacatgga tcgacgtccc gggtggttgc ccaccgggga cggtgtcctc gacacgcagc
     2041 gtgccgccat gtttgaccac aacctgcttc acgatcgcca ggccgaggcc cgatccgggc
     2101 atggcgcgcg ccgacgtcga acggtagaac cgctcgaaca ccagccgccg ctcctgcggc
     2161 gggataccgg gcccgaagtc agacaccacc agttcggcat gtgacgggtc gatttgcgtc
     2221 agccggacgc caacccgacc accgggcggg ctccacttcg cggcgttgtc gagcaggttc
     2281 agcaccgcgc gcgccaaccc accaccatcg ccgtagacct gccacccggt gaccgagacg
     2341 tcgaactcga tgtcgctgcg ccgtctgcga actcgctcaa gggagcggtc caccacgtcg
     2401 gacatgtcga ccggttcatg gatgacgcca ccggcctcgt cgcgggtcag atccaccaaa
     2461 tcgcctacca gcgttgacaa ttcctcgatc tgcgcgatca catccgcgcg cagcccggcc
     2521 atctcgtcgt ccggcaaccg cggcgcaccg ggcgccatcg cggccatcag cagctcgacg
     2581 ttggtacgca aggacgtcag cggggtgcgc agctcgtggc ccgcgtcggt gaccagtcgt
     2641 gcctggcgtt cgcgggattc ggccagcgca cgcagcatca tgttgaaagc ctctgtgaga
     2701 cgggcgagtt cgtcgctgcc gagcaccggg atgggtctca ggtcgtcggt tcgggccacc
     2761 cgttcggccg cttcggtcag ccgcgccacc ggccgtagcc cggcgcgggc caccgcacca
     2821 ccggccatcg cggccaccac aacgccgagg ccaccgacga tgagcagcac cgtgcccaac
     2881 cgtttgagca cctggctcgt cggcgccagg ctcttggaga tcagcagcga gctgccgttg
     2941 gtgaggtgca atgccagcac gcgctggtgg ttggcggtgc gcagagacat cagcaactcg
     3001 ccggacacca cagccttttc cggctccccg agcggcaatg tttggccttc ctggttagcc
     3061 gtatagatgt atcggctggg attgaccagc atcgcgttga catccgaata cgcagtgcct
     3121 tcgatggcct tgccgggatc ggcggccagc gacccgctct cgatcagcag ccgggcccgg
     3181 ctgtgcagct gattgtcgag gtcgtcgtag agcgcccgcg acaccaccgc gtagacggcg
     3241 acggaaatca gcaccacgac catcgccacc atcgacatgg cgagcagcat cacgcgccaa
     3301 cgcagcgaca ctgaactggc ggcggtaaga ggtggccgcc tcgcttgcgg cctgaacccg
     3361 aacttctggg ctgacatcag ggcggtgttt cacgcaacac gtaacccacc ccgcggacgg
     3421 tgtggatcag tcgcggctct ccttcggctt cggtcttgcg gcgcaggtat ccgacgtaca
     3481 cctcgagcgc attgccggac gtcgggaagt cgaaccccca gacctcctcg aggatgcggc
     3541 tgcgggtgag cacccggcgc ggattggcga tcagcatttc caacaaagcg aactcggtac
     3601 gagtcaggct gatctgccgg tcgccgcggg tgacttcgcg ggtcaccggg tccagggaca
     3661 gatcggagaa ggtcagcgcg ggcgactcgg catcgtcgtc gggcgtccgt cggcgcagca
     3721 gcgcgcgcat ccgcgccagc agttcctcga gtgcgaacgg cttgggcagg tagtcgtcgg
     3781 cgcccgcgtc cagcccagcg actcgctccg acaccgagtc gcgggcggtc agcaccagga
     3841 tcggcaggtc gtcgccggtg ctgcgaagct gacggcacac ctcgagcccg tccagcctcg
     3901 gcatcatcac gtcgagcacg agggcatccg gccggtcatt ggcgatcacg tcgagggcct
     3961 cgcggccgtc ctgcgccagc tcgactgaat atccgttgaa cgaaagcgac cggcgcaggg
     4021 attcgcgcac agcgcgatcg tcgtcaacga caagtatgcg cacagccaca gtgtctatcc
     4081 atcacctgag actgacctga gaggcgcgcc gctatcgcgc gcggccggcg ttagcggcgg
     4141 tcgaggtcga taaggccgag gcgggcggcc ttgagcaggc gccgcggcac cttgtgctgc
     4201 tgaccggcga cggtcacgtt gaccagttcg ggccgcttgg ccttccattg cgcgcgccgg
     4261 ctacgggtgt tcgcgcgcga cattctgcgc ttgggcacag ccatgatcga gctctcctca
     4321 tatagggggt atgcgcgccg cgcgtggtcg ggcgtcggca attgctcttc aggata
//
LOCUS       DASRJA010000432        10178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1235442,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000432 DASRJA010000000
VERSION     DASRJA010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10178
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235442"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFC01_18975"
     CDS             complement(<1..453)
                     /locus_tag="VFC01_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81743.1"
                     /translation="MTADRSVAVLAHGLGGSTDLPIPLSYALIGAAWALTFTFAVVAV
                     AWRRPRFDPDKPGRALPRWVTTVIDAPATRWVVAIAALLFAVWVAVAAVFGPQGPDNP
                     LPGVFYVLLWVGLVALSLAVGPVWRAISPVRTVHRLLGQWSLGLRYPEW"
     gene            complement(450..875)
                     /locus_tag="VFC01_18980"
     CDS             complement(450..875)
                     /locus_tag="VFC01_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81744.1"
                     /translation="MTVTSSRTLVILTAAALITAGCGGSHEVAVSTSSSQPPSTVNPS
                     DMSNEQAPPSRLLVDVTINGGKVTPANQQLQAAVKEQIVIRVNSDAADELHLHSTPEH
                     SFNIEAKPAQSFVFTVDVPGKVDVELHKLNKIIATITVQ"
     gene            complement(872..1051)
                     /locus_tag="VFC01_18985"
     CDS             complement(872..1051)
                     /locus_tag="VFC01_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81745.1"
                     /translation="MTGQPEVEILAHHGLLLAIPAFAPAIAVAGVVIYIAMRDRRRTS
                     DAPRGENSDSPRQDG"
     gene            complement(1048..2895)
                     /locus_tag="VFC01_18990"
     CDS             complement(1048..2895)
                     /locus_tag="VFC01_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81746.1"
                     /translation="MGTSHSSEQVSVLAQGGEGGGAALDQVIGMSVATLVVTAGLLWI
                     GYLHRQRRITWLNSLAEYAARKFKRPTWVALPMALFIATLICALFGFIWDVSLHIGKG
                     RDPGPLANPAHYFILFGLFLLFIAGCLACVLPYDKPGPSAVRITKDWYAPVGGILMAG
                     CGLYALLGFPLDDIWHRIFGQDVTLWGPTHLMMIGGAGFSTLSALMLNYEGLKAMGED
                     APTGGVFIKFVQYLGFGGILIGLSVFQIEFDFGVPQFRQVFEPMLIAAAAAFGCVAAR
                     IMLGRGAAVIAALLAIALRGLMALLVGPIFGAPINWFALYLGPAIVVELIALTPLFKR
                     PVLFGAVSGLGVATVGLWLESLWIGAVYHYPWPTSIWPEALAMAIPVAILTGACGALF
                     GLVLTSRRLPSRAISIGLVVLTVLAIGGATANGLRYNVPQNVTATIALTDVAGSAPGQ
                     RMVNADVLINPPTFVSDDPNWVSILGWQGHLQNQRGQVVDNLEKVGPGHYRTTQPVPV
                     WGSWKTLLRLHDGTTLAAVPIFLAADPGIGAKEVPAEASFTRPFVAEITILQRERNPD
                     TPMVLWTIGGLVVLFCTLVLIGVLSWGAGRINRIEASGSEAELQPTAQA"
     gene            complement(2980..4440)
                     /locus_tag="VFC01_18995"
     CDS             complement(2980..4440)
                     /locus_tag="VFC01_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HZN81747.1"
                     /translation="MDQSEAPILDGLVEYRKSNRYGYTPPGHRHGRGADDRVLAVLGH
                     EPFRDDVLMSGGLDDRRTSNKYLKRAEDLMAEAVGADLAFFSTCGSSLSVKAAMMAVA
                     GGDDSLLVARDSHKSVVAGLVFSGVQARWITPQWDAERHFSHPPSPQNYADAWARYPD
                     AAGALVVSPSPYGTCTDLEGIAKVCHEHGKPLIVDEAWGAHLPFHEDLPTWAMDAGAD
                     VCVVSVHKMGAGFEQGSVFHMQGDLVDKDRLSACADLLMTTSPNVMVYAALDGWRRQM
                     VEHGRELLGSALELVDGLRDDIELIPDVEVLDDELLGVEASHDLDRLQILMDLSGTGT
                     SGYQAADWLRKHKQVDLGMSDHRRLLATMSFADDKATGERLLDALWAWRKAANDFEPP
                     PPIRLPSPKEIELESIQLPRDAFFGDVEAVPAEKASGRISAEQITPYPPGIPAVVPGE
                     RLNDAVIDYLRSGLDAGMNVPDAADTSLKTFKVVVT"
     gene            4514..5266
                     /locus_tag="VFC01_19000"
     CDS             4514..5266
                     /locus_tag="VFC01_19000"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZN81748.1"
                     /translation="MRFEKRTAVITGGAIGFGRAFARALVAEGASVVIADIDVDTAER
                     TAAELVSAGGQAIAVDCDVADAERVDAAVATTIERFGGIDIVINNAGLHLMKYNQPFG
                     VLSRNEIRSLFDVNVMGVINVTLACRDSMHERGGGVVLNISSMAGYMSATPYAVSKLT
                     VRGLTVAFATELAPDRIRVNAIAPGLMNTENALADLPKPLIDEFVHKLQLVHRLGTMD
                     DVVSAMLFLCSDEASFITGETFKVSGGYPLNF"
     gene            complement(5248..6024)
                     /locus_tag="VFC01_19005"
     CDS             complement(5248..6024)
                     /locus_tag="VFC01_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZN81749.1"
                     /translation="MTFRDLHYGELPLLLPNAWDVPSALALLECGFAAIGTTSFGVAS
                     SLGRPDGGRSTKEANLGLAAALRPLDCYLTMDIEDGYTDDPDEVADYVGRLPVDGINI
                     EDSTDAKLIDPPRNAAKVQAIKQRSPDVFVNARVDTYWFGQDATVAATVDRAARYAEA
                     GADGIFIPGAAEPDLLRELAAAIPLPVNVLAIPGLSLAELAKLGISRVSTGSLPYRVA
                     IHAAAQAAVAVRDATELPASDPYPEMQARLVRYTNQKFSG"
     gene            complement(6021..6851)
                     /locus_tag="VFC01_19010"
     CDS             complement(6021..6851)
                     /locus_tag="VFC01_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HZN81750.1"
                     /translation="MAVRMMDLLSGNFDTLRYEPTAKRIRAYIGGEPVAETCEARLVW
                     EPRRVVPTYAVPIDALTAQLVPAGAESGADEDVGVRLPAISSRPILDPSVPFDAHSCA
                     GTAYDVIAGEETGAAAAFRPDDPDMADYVILEFAAFEWREEDEPLVGHPHDPFGRIDV
                     LRSSRHVRIELDGRLLAESTRPMVLFETLLPVRFYLPREDVAVRLEPSDRASYCAYKG
                     RASYYSVPDGPRDVAWTYHEPLHDAEPVRDRICFFDERVDMIVDGERRDRPITPWSDE
                     "
     gene            complement(6864..8258)
                     /locus_tag="VFC01_19015"
     CDS             complement(6864..8258)
                     /locus_tag="VFC01_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN81751.1"
                     /translation="MSFDITPTAAQHDLARRTHEFAEQCVRPVALEYDQRQEFPWPVL
                     EEAAERGFYSPLFYRDLIGDPTGLSLPMFMEELFWGCAGIGLAIVMPALALSAIGQAA
                     SPEQMLQWAPECFGTPGDLKLAALAISEPEGGSDVRNLRTRAHRDGSEKDADWIIDGH
                     KMWIGNGGIANVHVVNAVVDEELGHKGQALFIVPGGTPGLELVRKLDKLGCRASHTAE
                     LKFNGVRVPAENLLGGQEKLEHKLAKAREAMEGAKHSGSATMGTFEQTRPMVAAQALG
                     IARAALEYATEYANRREAFGAPIIDNQGVAFPLADLATAIDAARLLTWRASWMAASGV
                     PFERGEGSMAKLAASEVAVRTTERAIQTMGGWGYIKDHPVEKWYRDAKLYTIFEGTSE
                     IQRVVISNALGAADGKPPLHVHLEPTGGPLNKWFGRGTPLRSRAADAALSAKDRVPRP
                     IMRLAMNVLRPPRK"
     gene            complement(8303..8515)
                     /locus_tag="VFC01_19020"
     CDS             complement(8303..8515)
                     /locus_tag="VFC01_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005058188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HZN81752.1"
                     /translation="MRLSIRNQLTGVITEVNLGAVMATVKIRLDGSEQIITSSITKDA
                     AEDLGLQTGMRATAFVKSTEVAIGVD"
     gene            8548..9081
                     /locus_tag="VFC01_19025"
     CDS             8548..9081
                     /locus_tag="VFC01_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81753.1"
                     /translation="MKLAAMNLRQRDGVTCGPTVAVVAGALLDPGNQAALSDRAWFAA
                     EQARLHAELNRVWPRRLGTTPAGMARALTGHSAASGVRYRWRLFRGRRDGLADVRRAV
                     AKGWPVAMLIGWFVPRHWVLIIEADGQRWQCYEPSSGQVRAVERDAVQRSRLTGLGYP
                     RPFAFVLPRASTRSATM"
     gene            complement(9021..9659)
                     /locus_tag="VFC01_19030"
     CDS             complement(9021..9659)
                     /locus_tag="VFC01_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HZN81754.1"
                     /translation="MYIPRQFALSDEETEAALAQAGFAHLVTQDPSGLMVTPLPLLYN
                     QANHSLIGHVSRANPHWHADSRESVAIFSGPQAYISPSFYATKAETGKVVPTWNYEVL
                     NVYGRLIVHDDADWVLNLVTTLTNSHEQQRTEPWQVTDAPETYTRAQLRAIVGMELMI
                     SKVEGKAKMSQNQPERNREGVVAGLKASDTPHDHIVADRVDALGRTKANGRG"
     gene            complement(9896..10078)
                     /locus_tag="VFC01_19035"
     CDS             complement(9896..10078)
                     /locus_tag="VFC01_19035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81755.1"
                     /translation="MSDTETSPVTLLDGLASLDPEARRELATNLRQVSATFHEVPDGR
                     TTSHVLGVLAHLLDPS"
BASE COUNT         1710 a         3258 c         3458 g         1752 t
ORIGIN      
        1 ccattcggga tatcgcagac ccagcgacca ctggcccagc agtcgatgca cggtccgcac
       61 cggcgagatc gcgcgccaca cgggtccgac ggcgagcgac agcgccacca gaccgaccca
      121 caacagcacg tagaagacgc cgggcagcgg gttgtccggg ccctgcgggc cgaacaccgc
      181 ggcgaccgcg acccagaccg cgaacagcag cgccgcgatc gccaccaccc agcgggtggc
      241 cggcgcgtcg atcaccgtcg tcacccatcg cggcagtgcc cggcctggct tgtccgggtc
      301 gaaccgcggc ctgcgccagg caaccgcgac aaccgcaaaa gtaaacgtca acgcccacgc
      361 agcgccgatg agcgcatagc tcaacgggat tggcagatcg gtcgagccac ccaggccatg
      421 ggccagtacg gcaacggacc gatcagccgt cactgcacgg tgatggttgc gatgatcttg
      481 ttcagcttgt gcagctccac gtcgaccttg ccgggcacgt cgacggtgaa cacgaaggac
      541 tgcgccggct tggcctcgat attgaagctg tgctcgggcg ttgagtgcaa gtgcaactcg
      601 tcggcggcgt cgctgttgac gcgaatgacg atctgctcct tgaccgcggc ttgcagctgc
      661 tggttggccg gggtcacctt gccgccgttg atcgtgacgt cgacgaggag ccgcgacggc
      721 ggcgcctgct cgttcgacat gtccgacggg ttgacggtcg acgggggctg gctgctcgag
      781 gtgctgacag ccacctcgtg agatccacca catccggccg tgatgagggc agcggccgtc
      841 aagatcacaa gagttctaga tgaagtcacg gtcaaccatc ttggcgcggc gagtcggagt
      901 tctcacctcg gggtgcatcg ctcgtgcggc ggcggtcccg catcgcgatg tagatgacca
      961 cccccgcgac ggcgatcgcg ggcgcgaagg ccggtattgc aagaagcagc ccgtgatggg
     1021 cgagaatctc aacttcgggt tgtccggtca cgcctgcgcc gttggctgga gttcggcctc
     1081 gctgcccgag gcctcgatgc ggttgatcct accggcgccc cagctgagga cgccgatgag
     1141 gaccagcgtg cagaacagga ccacgaggcc gccgatcgtc cacagcacca tcggggtgtc
     1201 ggggttgcgc tcgcgctgca ggatggtgat ctcggcgacg aacggccggg tgaacgatgc
     1261 ctctgccggc acctcttttg cgccgatgcc cgggtccgcg gcaaggaaga tcggcaccgc
     1321 cgccaacgtc gttccgtcat gcaggcgcag gagcgtcttc cacgaacccc acaccggaac
     1381 gggctgggtg gtgcggtagt gaccgggacc gaccttttcg agattgtcga cgacctggcc
     1441 acgctggttc tgcagatggc cctgccagcc gaggatcgac acccaattgg ggtcgtcgct
     1501 gacgaaggtc ggcggattga tcagcacgtc ggcgttcacc atccgctgac caggcgcgct
     1561 gcccgccaca tccgtgagag cgattgtcgc cgtgacgttt tgcggcacgt tgtagcgcag
     1621 gccgttggcg gtcgcgccac cgatcgccag gactgtcagc accacaagac cgatgctgat
     1681 ggcgcgcgac ggcagtcgcc gcgaggtgag caccaagccg aacagggcgc cgcacgcgcc
     1741 tgtgaggatg gcgaccggga tggccatggc cagcgcctcg ggccagatgc tggtcggcca
     1801 cgggtagtgg tagaccgcgc cgatccacaa tgactccaac cagaggccga cggtcgccac
     1861 acccaagccg ctgacggcgc cgaaaagcac gggccgtttg aaaagtggtg tcagcgcgat
     1921 caattcgacg acgattgcgg gtccgaggta cagcgcgaac cagttgatcg gcgccccgaa
     1981 gatcgggccg acgagtagcg ccatcagacc gcgcagcgcg atggccagca gcgcggcgat
     2041 cacggccgca ccgcgtccca gcatgatgcg ggccgcgacg cagccgaatg cggccgccgc
     2101 ggcgatcagc atgggttcga acacctgtcg gaattgcggt acgccgaaat cgaattcgat
     2161 ctggaacacc gacaggccga tgagaatccc gccgaagccg aggtactgaa cgaacttgat
     2221 gaagacgccg ccggtcgggg cgtcctcacc catcgccttc agcccctcgt aattcagcat
     2281 gagtgcggac agcgtcgaga agcccgcgcc accgatcatc atcaagtggg tcggacccca
     2341 cagcgtgacg tcttggccga agatgcggtg ccagatgtcg tcgaggggga agccgagcag
     2401 tgcgtagagg ccgcagccgg ccatcaggat gccgccgacg ggcgcatacc agtctttcgt
     2461 gattcggacg gcagatggac ccggcttgtc gtagggcagc acgcaggcca ggcagccggc
     2521 gatgaacaac aggaacaagc cgaacaggat gaagtagtgc gcggggttgg ccagcggccc
     2581 cgggtcgcgg cccttgccga tgtgcaggct gacgtcccag atgaacccga acagcgcgca
     2641 gatcagtgtc gcaatgaaca acgccatcgg cagcgccacc cacgtcggcc gcttgaattt
     2701 cctggccgca tattccgcca ggctgttcag ccaggtgatg cggcgctgcc ggtggagata
     2761 gccgatccac agcaacccgg cggtcacaac gagcgtggcc accgacattc cgattacctg
     2821 atcgagcgcc gcgccgccgc cctcaccgcc ctgcgcgagc accgaaacct gctctgagct
     2881 gtgcgaagtc cccattgcca tcccacccgc tgactgtcgt cggaccgaac tgactcacga
     2941 gtaatgttgc cagaattccc cgaaccgaaa ccaggggtat caggtaacta ccaccttgaa
     3001 agtcttcaaa ctggtgtcgg cggcgtccgg cacattcatc ccggcatcga ggccggagcg
     3061 caggtagtcg atcaccgcgt cgttgagccg ctcgccgggc accaccgcgg gtatgccagg
     3121 cggatagggc gtgatctgct cggcggatat ccggccgctg gccttctcgg ccggcactgc
     3181 ctcgacgtcc ccgaagaacg cgtccctcgg cagctggatg ctctcgagtt cgatctcctt
     3241 cggcgacggc aaacggatcg ggggcggcgg ctcgaagtca ttggctgcct tgcgccacgc
     3301 ccacagcgca tccagcagcc gttcgccggt ggctttgtcg tcggcgaatg acattgtcgc
     3361 cagcagccgg cggtgatcgc tcatgccgag gtcgacctgc ttgtgcttgc gcagccagtc
     3421 agcggcctgg tagcccgacg ttcccgtgcc ggacagatcc atcaggatct gcaaccggtc
     3481 caagtcgtgg gatgcctcga caccgaggag ttcgtcgtca agcacctcga cgtcgggaat
     3541 caactcgatg tcatcgcgca gcccgtccac caattccagt gcgcttccga gtaattcgcg
     3601 gccatgctcg accatctgcc gccgccagcc atccagtgcc gcgtacacca tcacgttcgg
     3661 gctcgtggtc atcagcaggt ccgcgcatgc cgacagccgg tccttatcga ccaggtcgcc
     3721 ctgcatgtgg aacaccgagc cctgctcgaa cccggcgccc atcttgtgca cgctgaccac
     3781 gcacacgtcg gcgccggcat ccatggccca ggtcggcagg tcctcgtgga acggcaggtg
     3841 tgcaccccag gcttcgtcga cgatcagcgg cttgccgtgc tcgtggcata ccttcgcgat
     3901 gccttcgagg tcggtgcagg tgccatacgg gctggggctg accaccaggg cgccggcggc
     3961 gtcggggtag cgggcccagg cgtcggcgta gttctgcggc gacggcggat gggagaagtg
     4021 ccgctcggca tcccactgcg gcgtgatcca ccgcgcctgc acgccggaga agacgaggcc
     4081 ggccacgacc gatttatggc tgtcacgtgc gaccagcagg ctgtcatcgc cgcctgcgac
     4141 ggccatcatc gcggccttca ccgacaaaga gctgccgcat gtcgagaaga acgcgaggtc
     4201 ggcgccgacg gcctcggcca tcaaatcctc ggcccgcttc aagtacttgt tgctggtgcg
     4261 ccggtcatcg aggccgccgc tcatcaacac gtcgtcgcgg aagggctcgt gcccgagcac
     4321 cgccagcacc cgatcgtccg cgccgcggcc gtgacgatgg ccgggcggcg tgtacccgta
     4381 ccggttgctc tttcggtatt cgaccagtcc gtcgaggatc ggcgcttcgg attgatccat
     4441 gatcggcgga gtacccgttt actgcgtgag caccacattg accgcgcaga cgacagcggt
     4501 gagaatgcca gcgatgagat tcgagaagcg caccgcggtg atcaccggcg gtgcgatcgg
     4561 cttcggccgg gcgttcgcgc gggcgctggt cgccgagggc gccagcgtgg tgatcgccga
     4621 catcgacgtt gacacggccg aacgcactgc agcggaactg gtttcagccg gcggacaggc
     4681 gatcgcggtg gactgcgacg tcgccgacgc ggaacgggtg gacgccgccg tagcgacgac
     4741 gatcgaacgg ttcggtggca tcgacatcgt gatcaacaac gccgggctgc acctgatgaa
     4801 gtacaaccag ccattcgggg tgttgtcccg caatgagatc cgcagccttt tcgacgtcaa
     4861 cgtgatgggc gtgatcaacg tgacgctggc ctgccgggac tcgatgcacg agcgcggtgg
     4921 cggcgtggta ctcaacatct cgtcgatggc cggctacatg agcgccaccc catatgcggt
     4981 gtcgaaacta acggtgcgcg ggttgaccgt cgcgttcgcg accgaacttg cgccggatcg
     5041 aatccgcgtc aacgccattg cacccggact gatgaacacg gagaatgcgc tggccgatct
     5101 gccgaagccg ttgatcgacg agttcgttca caagcttcag ctcgtgcacc ggctcggcac
     5161 gatggacgac gtggtctcgg cgatgttgtt cctgtgctcc gacgaggcgt cgttcattac
     5221 gggggagacg ttcaaggtca gcggcggcta cccactgaat ttctgatttg tgtagcgcac
     5281 gagacgcgct tgcatctcgg gatacgggtc ggaagctggc aactcggtgg catcgcggac
     5341 ggcgaccgcg gcctgcgccg cggcgtgaat ggcgacgcgg tacggcagtg aacccgtcga
     5401 cacccggctg atgccgagct tggcaagctc agcgagcgag agtccaggga tggcaaggac
     5461 attgaccggc agcgggatgg ccgcggcgag ctcgcgcagc aggtcgggct cggcggcgcc
     5521 gggaatgaag atgccgtccg cgcccgcctc ggcgtatctg gcggcccggt ccactgtcgc
     5581 ggccacggtg gcgtcctgac cgaaccagta ggtgtcgacg cgggcgttga cgaacacatc
     5641 gggcgaacgt tgtttgatgg cttgcacttt ggccgcattc cgcggcgggt cgatcagctt
     5701 cgcgtcggtg ctgtcctcga tgttgatgcc gtcgacgggc agtcggccga cgtagtcggc
     5761 gacctcatct ggatcgtctg tgtagccatc ttcgatgtcc atggtgaggt agcagtcgag
     5821 tggccgtagc gcggcggcca gcccgaggtt ggcctccttg gtcgaccggc ctccgtctgg
     5881 cctgccaagg ctggacgcca cgccgaagct ggtggtgccg atcgcggcga agccgcactc
     5941 cagcagcgcc agcgccgacg gtacatccca cgcgttgggc agcagcagtg gcagttcgcc
     6001 atagtgcaag tccctgaacg tcattcgtca ctccacgggg tgatcggccg gtcgcggcgt
     6061 tcgccgtcca cgatcatgtc gacgcgctcg tcgaagaagc agatccggtc gcgcacgggt
     6121 tcggcgtcgt gcagtggctc gtgatatgtc cacgccacgt cgcgtgggcc gtcgggcacg
     6181 gagtagtacg acgcccggcc cttgtatgcg caatagcttg ccctgtcact gggttcgagc
     6241 cggacggcca cgtcctcgcg aggcagatag aaccggaccg gcagcagcgt ctcgaacagc
     6301 accatcgggc gcgtggactc cgccagtagc cgaccgtcga gttcgatgcg gacgtggcgg
     6361 ctgctgcgca gcacgtcgat gcggccgaac ggatcatgcg ggtggccgac caacggctcg
     6421 tcctcctcgc gccattcgaa cgcggcgaac tcgaggatga cgtagtcggc catgtcggga
     6481 tcgtcgggcc ggaacgcggc cgcggcgccc gtctcctcac cggcgatgac gtcgtacgcg
     6541 gtgcccgcac acgaatgcgc atcgaagggg acgctcgggt cgaggatggg gcgcgaactg
     6601 atcgcgggga ggcgcacgcc gacgtcctcg tcggcgccgg actcggcgcc cgcgggtact
     6661 agctgcgcgg tcagcgcatc gatcggtacc gcataggtcg gcacgacgcg gcggggctcc
     6721 cacaccagcc gggcctcaca ggtttcggcg accggctcgc cgccgatgta tgcccggatc
     6781 cgtttcgcgg tgggctcgta gcgcagggtg tcgaaattgc cgctgagcag atccatcatt
     6841 cgtacggcca tcgcggaccc ctttcacttg cggggcggac gcaacacgtt cattgccaag
     6901 cgcattatcg gccgagggac gcgatccttg gcggaaagcg cggcatccgc cgccctgctg
     6961 cgcagcggag tgcccctgcc gaaccatttg ttcagtgggc cgccggtcgg ctcgaggtgc
     7021 acgtgcaggg gcggcttgcc gtcggcggcc ccgagcgcgt tggagatgac gacccgctgg
     7081 atttcgctgg tgccctcaaa gatggtgtac agcttggcgt ctcggtacca cttctcgaca
     7141 ggatggtcct tgatgtaacc ccagccgccc attgtctgga tcgcccgctc ggtggttctt
     7201 accgcgacct cgctggctgc cagtttggcc atcgagccct cgccgcgctc gaacggcacg
     7261 ccggacgcgg ccatccagga tgcccgccag gtgagcaggc gtgccgcgtc gatcgcggtg
     7321 gccaggtcgg cgagcgggaa cgcgacacct tggttgtcga tgatcggcgc accgaacgcc
     7381 tcccgccggt tggcgtactc ggtggcgtat tccaatgcgg cccgcgcgat tccgagcgcc
     7441 tgggcggcga ccatcggccg ggtctgctcg aaggttccca tggtggccga tccggaatgt
     7501 ttggcaccct ccatcgcttc ccgcgccttg gccaacttgt gttcgagctt ttcttggcca
     7561 ccgagcagat tctcggccgg aacacggaca ccgttgaact tcagctcggc ggtgtgcgac
     7621 gcgcggcagc caagcttgtc gagcttgcgg accagttcca gacccggcgt gccgccgggc
     7681 acgataaaca gggcctgccc cttgtgaccg agctcctcat cgacgacggc gttgacgacg
     7741 tgcacgttgg cgatgccgcc gttgccgatc cacatcttgt gaccgtcgat gatccagtcc
     7801 gcgtcctttt ccgagccgtc gcggtgcgcc cgggtgcgca ggttgcgaac atcgctaccg
     7861 ccctcgggct cggagatcgc cagcgcggcc agcttgagat cgcccggggt gccgaaacat
     7921 tcgggtgccc actgcagcat ctgctcggga gaggcggcct ggccgatagc cgagagcgcc
     7981 aaggcgggca tcacaatcgc caggccgatg ccggcgcagc cccagaacag ctcttccatg
     8041 aacatcggca gcgacagtcc ggtcggatcg ccgatcaggt cgcggtagaa gagcggactg
     8101 tagaacccgc gttcggccgc ctcttcgagc accggccatg gaaactcctg gcgctggtcg
     8161 tactcgagcg cgacggggcg gacgcactgt tcagcgaatt catgcgtgcg tcgggcgagg
     8221 tcgtgctgcg cggcggtggg cgtgatgtcg aagctcatcg actgccgggt accccgcggc
     8281 agcgctggcg aagcgctggg cgctaatcga cgccgatggc gacctctgtc gatttcacga
     8341 atgcggtcgc gcgcatcccg gtctgcaggc cgaggtcctc cgcggcgtcc ttggtgatcg
     8401 aggacgtgat gatctgctcg ctgccgtcga ggcggatctt cacggtcgcc atcaccgctc
     8461 ccaggttgac ctcggtgatc acgccggtga gctggtttcg gatcgataga cgcatagtgc
     8521 ctgagcgtag gcctaccgtg atggtcgatg aagctcgcag ccatgaacct tcggcaacgc
     8581 gacggcgtca cgtgcggacc gaccgtcgcg gtggtggcgg gcgccctgct ggatccgggc
     8641 aaccaggcgg cgctgtccga ccgggcgtgg ttcgccgccg aacaagcgag gctgcatgcg
     8701 gagttgaacc gggtctggcc gcgccggctg ggcaccacgc cggcagggat ggcgcgtgcc
     8761 ctcactgggc atagcgccgc atcgggagtg cgttaccggt ggcggctgtt tcgcggtcgg
     8821 cgggacgggt tggcggatgt gcggcgcgcg gtggcgaagg gctggccggt ggcgatgttg
     8881 atcgggtggt tcgtccctcg gcattgggtg ctgatcatcg aagcagacgg acagcggtgg
     8941 cagtgttacg aaccgtcgtc gggacaggtg cgcgcggttg agcgggatgc ggtgcagcgg
     9001 tcgcggctga ctgggttggg ctacccgcgg ccgttcgcct tcgttcttcc gagagcgtcg
     9061 acacggtcgg cgacgatgtg atcgtgtggt gtgtcggacg ctttgaggcc ggcgaccacg
     9121 ccctcgcggt tgcgttcggg ttggttctgc gacatcttcg ccttgccttc gactttcgag
     9181 atcatgagct ccatgccgac gatcgcgcgt agttgtgcgc gcgtgtaggt ttcgggtgca
     9241 tcggtgacct gccacggctc ggtacgttgc tgttcgtgac tgttcgtcag cgtcgtgacg
     9301 aggttgagca cccagtcggc gtcgtcgtgc acgatgaggc ggccgtagac gttgaggacc
     9361 tcgtagttcc aggtcggcac caccttgccc gtttcggctt tcgtcgcata gaagctgggc
     9421 gagatgtagg cctgcggacc ggagaagatg gcgaccgact cgcggctgtc ggcgtgccag
     9481 tgcgggttgg cccgtgacac gtgaccgatg agcgaatggt tggcctggtt gtacagcagc
     9541 ggcagcggcg tgaccatcaa ccccgacggg tcctgggtga ccagatgcgc gaagccggcc
     9601 tgggcgagag cggcttcggt ttcctcgtcg gagagggcga actgtcgggg tatatacatg
     9661 gcaggttcag cgtaggccca tcggcaactc agatttgctg ttcgaacgta tgttctccag
     9721 ggtcgccgat gaactgtcct gaatgtccat agagtgcttc gctgtagcac cgacggcctc
     9781 gtagtgacct gttatctggg tgtctacaac gggagctctc agagattggg cgcaaccgtg
     9841 acgaagtttc gagactctca gaagtcgtca tattcgagac aggtccacaa gctggtcagg
     9901 acgggtccag caggtgagcc aggacgccga gcacgtgtga ggtcgtcctg ccgtcaggca
     9961 cctcatggaa ggtggcagat acctgccgga ggttagtggc cagttcgcgg cgggcctccg
    10021 gatcgaggct cgcgagtccg tcaagtagcg tcaccggcga cgtctccgtg tcagacatag
    10081 cttgacttcc gcgcctaggc ctcggccttg gcacgctgcg catcgatctc gcgcagctcg
    10141 acacgccgga ccttgcccgt cgcggtgcgc ggcagcgc
//
LOCUS       DASRJA010000433         5993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3667864,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000433 DASRJA010000000
VERSION     DASRJA010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3667864"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VFC01_19040"
     CDS             <1..817
                     /locus_tag="VFC01_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN81756.1"
                     /translation="RTDDRNLLQQMVNDARDAYPTGSSVVDRGTAYVLALAAWQREDV
                     QGAMRWLGGDITIFGTPFFPQVLDQVILGARVASAAGDAGLRERVLQAADLLERERPA
                     IPLFTGVAGYARGILERDAPALVAAAESFQSSSRPLLCAAAAEDAGGQLADTGRGDEA
                     LDQLNAGFDIYMHHGALADARRVGRELRRLGVQRRIVSRPRPKMGWDSLTDSELTVVN
                     LIAQGATNRSVAERLHVTPHTVKAHLHNAFAKLGINSRAELTKLTLGNDRPTN"
     gene            919..2502
                     /locus_tag="VFC01_19045"
     CDS             919..2502
                     /locus_tag="VFC01_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN81757.1"
                     /translation="MRSHSNDGRGYARRRAREYTRKRAKNAECSAWNAGYDAGGVRCR
                     WVGDGVPMTADSTSCVAESASSTIDPDVQAGRASDGELNAGFYSDVTIDEDAIEFNEN
                     LDDGELYAKYRRLAAEQAALRRLTTLVARGVEPLEVFGAVAEEMRRCVPADVAGLWRF
                     ESDRETTVVVSAAEPETLASWQVGTRTPLEGNTIAALVQRSGRPARIDSFDNLAGPIA
                     ARLRAVGVSAAVGVPIIVDGRVWGLTAVGTLQHGPMPADTEVHVSGFAALIAATLVAG
                     YRAEQTRQLLAEASQRLSLIDSLLEGLALNDWSLWQVAGKLRLPINGPFVVVAAHGPA
                     VGDEPLPEIESKLRSLDIFSAWRRLPDMQVGIVHVASDQKLGKVVALMSRMTTARVGV
                     SAAFTDLRDTPHALHVARVMLRGPTDSTSSVAVFDGSLLATAAGSAPEVMIKTAGAAL
                     DGFGDLPDEDRETLFQTFRVWQDNGASVRSAAEVLICHPNTVRHRLRRIEKRTGRSLA
                     RPRDVAELCLAFEVHRRLM"
     gene            3050..4387
                     /locus_tag="VFC01_19050"
     CDS             3050..4387
                     /locus_tag="VFC01_19050"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZN81758.1"
                     /translation="MTLFEIVPSLRHAVTPRIDRAIWPLTTRVDELGRLCVGEVALTE
                     VADEFGTPTYVIDEADFRHRIRRYRAALQDARLVYAGRALLTTAVARWAAEEGAGLGV
                     RSGGELATALIAGVEPKQIVVHGMATSPAALCEVANAGVGRVVVESPTEVAFLAADLR
                     RPQAVQIRVSPDIDWAANVIQRALDQPMLHLVGLQCHIGSHVTEAPLYGEAIRQMIPL
                     MAEIRARHGVILTELNIGGGQGVPHVSGDPELDLEAMRDFVEDSLDASCAAERFPRPT
                     VVVEPGRAISARAGVTLYRVLWVQSQPGGRTIVVVDGGLSDSPQVARRASYTVAVANR
                     HPMTATQPMTVVGRQCESGDEIARDVPLPADLHAGDLLAVACTGAYHHSMASTYNMVG
                     RPPLVAVKDGRTRELIRRETIADLLSRDRVYKERPPMRRGRIESGCEALLHGE"
     gene            4531..5130
                     /locus_tag="VFC01_19055"
     CDS             4531..5130
                     /locus_tag="VFC01_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HZN81759.1"
                     /translation="MNSLVGQRLWKWKLVAGLLTIVLGALVLVWPGPSILVAATLFGV
                     YLLLSGMVELFLAFTLPRSAGTRVMLFISGALSLVLAILSFRHFGDGYAVLLLSLWIG
                     IGFIFQGVTGVAVGIGESELPGRGWYVVAGIISVIAGLVVLVWPFASIAVLTLAAGIW
                     LVIIGITQIVQAVQTRKAAKTAHEALDEVSNRIKTALSD"
     gene            5150..5575
                     /locus_tag="VFC01_19060"
     CDS             5150..5575
                     /locus_tag="VFC01_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007298492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81760.1"
                     /translation="MTTSSRDVDNRSGQESPSGATAMIAAGALMLTSAVLTFLIGVFA
                     LAADDLVVSGPGYEYTFQMTGWGWANILTGMVLAGVAIALLSSAQWARAAAIVVTCLA
                     IVVTFLWMPYYPTGSIVLIALDVVVIWGVATWNTARKTA"
     gene            5572..>5993
                     /locus_tag="VFC01_19065"
     CDS             5572..>5993
                     /locus_tag="VFC01_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZN81761.1"
                     /translation="MKPPPAKPEKSRRAWAVSRKQFALNGTLADRVISVAHIIGRPVR
                     NVAGTRIGRVSDIVVRWDADNEHPPVTGVFVKVRGGLATAQVADVTLTQTEIRLRSDA
                     RMQWRPVWADDDVALARDVLDRQLVDTSGAQVVRAADA"
BASE COUNT         1042 a         1820 c         1969 g         1162 t
ORIGIN      
        1 gcgcaccgat gaccgaaact tgttgcagca gatggtcaat gacgcgcgcg acgcctaccc
       61 cactggctca tctgtggtag atcggggaac ggcgtacgtg ctggcgctgg cggcgtggca
      121 gcgcgaggat gtccagggtg cgatgcgctg gctaggcggc gacatcacca tattcggaac
      181 accgttcttc ccccaggtct tggatcaggt gatcttgggt gcgcgggttg cgtcggccgc
      241 cggcgatgcc ggtttgcgcg aacgtgtcct gcaggccgcg gacctgctcg agcgtgagcg
      301 gccggcgatc ccattgttca ccggggtggc cgggtatgcg cgcgggatac tcgaacgtga
      361 cgctccagcg ttggtggctg ccgcggagtc gtttcagtcg tcgtcgcggc cgcttctctg
      421 tgccgcagct gccgaggacg ccggtggcca actcgcagac accggccgcg gtgacgaggc
      481 gctcgatcag ctcaatgccg gattcgacat ctacatgcac cacggagccc tcgccgatgc
      541 gcgtcgggtg ggccgcgagt tgcgccggct cggtgtgcaa cgtcgcattg tcagccggcc
      601 gcggccaaaa atgggctggg acagcctcac cgactccgag ctcacggtgg tcaacctgat
      661 tgcgcagggt gctaccaaca ggtccgtggc agagcggctg cacgtcacgc ctcacacagt
      721 gaaagcccac ctgcacaatg cgttcgccaa gctcgggatt aattcccgcg ctgagctgac
      781 caaactcacg ctgggcaacg accgccccac caattagatt gtggcgactt agctattacc
      841 cggcctggcc ttaaagattg cccgccgggc aatggtgtca tccacacccg cgcggcgacc
      901 gtagtagtcg tcaactaatt gcgttctcac tcgaacgacg gccgaggcta cgccagacgc
      961 cgcgctcgtg agtacacgag gaagagagcg aaaaatgctg aatgcagtgc ttggaatgct
     1021 ggctatgatg ccggtggtgt tcggtgtcgt tgggtgggcg atggtgtccc gatgaccgca
     1081 gactcgacta gctgcgtcgc cgaatcagca agtagcacta tcgatcccga cgtgcaggca
     1141 ggacgcgcga gcgacggcga actgaacgca gggttctact ctgatgtgac gattgatgag
     1201 gatgctatcg aattcaacga gaacctggac gatggcgagt tgtacgccaa gtaccgtcgg
     1261 ctcgccgccg aacaggccgc cttgcgccgc ctcaccacgc tcgtcgcacg cggcgttgaa
     1321 cctttggagg tattcggcgc ggtggccgag gagatgcgtc gatgcgtgcc cgcagacgtc
     1381 gccggattgt ggcgcttcga atcggatcgc gagactaccg tcgtggtctc cgctgccgag
     1441 cccgagaccc tggcaagttg gcaggtgggc acgcgaactc cgttggaagg caacaccatc
     1501 gccgcgctgg tgcaacgcag cggccggccc gcacgaatcg acagcttcga caacctcgcc
     1561 gggccgatcg ccgcgcgctt gcgcgcagtg ggtgtgagtg cggcggtagg cgtgccgatc
     1621 atcgttgatg gacgcgtgtg gggcctgacg gccgtcggta ccctgcaaca cggccccatg
     1681 ccagccgaca ccgaggtcca cgtcagcggg ttcgctgcac tgatcgccgc tacactcgtg
     1741 gccggttatc gcgccgagca gacacggcag ctcctggctg aggcgtcaca acgtttgagt
     1801 ctgattgact cgctactgga gggattagct ttaaacgatt ggagcctgtg gcaggtcgca
     1861 ggcaagctac ggctgccgat caatggtccg tttgtggtcg tcgccgccca cggtcccgcg
     1921 gtgggcgatg agccgcttcc cgagatcgaa tcgaagctac gcagccttga catcttttca
     1981 gcgtggcggc ggctgcctga catgcaggtc gggatcgtgc acgtcgcgtc cgaccaaaaa
     2041 ctcggcaaag ttgttgccct aatgtcgcgg atgaccacgg cgcgggtggg cgtcagcgcg
     2101 gccttcacgg atctgcgcga tacgccacat gccctgcatg tcgcaagagt gatgttgcgg
     2161 gggccgaccg attccacatc ctcagtcgct gtgttcgacg gctccctcct ggccaccgcg
     2221 gctggcagcg cgccggaggt gatgatcaaa acggccggcg ctgcgttgga cggtttcggc
     2281 gaccttcccg acgaggaccg ggagacgctt tttcaaacgt ttcgggtgtg gcaggacaat
     2341 ggtgcgtcgg tgcgcagcgc agctgaggtg cttatttgcc atccgaatac ggttcgccac
     2401 cgattgcgcc gaattgagaa acgcaccggc cgatccctcg cgcggccaag ggatgtcgcc
     2461 gagctatgtt tggctttcga agtgcaccgt cggctcatgt aaccacgtgg acggttgtct
     2521 acgtcagcga tgcccttgcg gcaagagcga atccagaagt agggatcatg ttgtgcgcat
     2581 tattgattgg tgcattagcc atcgtggtgg tggtgagtca gatagccatc gtgatgtgct
     2641 gccacgcaca ggcccccacc gtacgcgatc agtacgagca ctgactcatg aatccgcaga
     2701 ccggtgttgc catgacgacc ggcataaacg actcgacgag catcgtggtt gggtccggga
     2761 gtcgttccca ttgcgagcgt ggtgccgcgc cgagagctgc agcaattgcc ttgtaccgca
     2821 ttgattctgc gatctcagta gttgtatcgc agcagcgacg ccgtcccctt gtcagcgtcg
     2881 gctgttggac aactcgatcg tccaacgagt ggtagagccc gtcgatgtgg ttagacacaa
     2941 cggttgttgc actgcgttgt tagcggcgac atcgacttcg gcgtcaacat cgctcaacta
     3001 ttgaataacg ttgttgggca ataactgtgg gaagtggaat ccagattcga tgactctctt
     3061 cgagatcgtg ccgtcgctgc ggcacgccgt tacgccgcga atagaccggg cgatatggcc
     3121 cctgaccacc cgcgtcgatg agctgggtcg gctttgtgtc ggtgaggtcg cgctgaccga
     3181 ggtcgccgat gagttcggca ccccgacata tgtcatcgac gaggccgact ttcgtcatcg
     3241 gattcggcgg taccgcgcgg cgctccagga cgctcgcctg gtctacgcag gaagggcgct
     3301 gctgaccacc gcagtcgcac gatgggcggc ggaggaggga gcaggtcttg gcgtgcgctc
     3361 cggtggcgag cttgcgactg cgctcattgc gggcgtggaa ccgaaacaaa tcgtcgtcca
     3421 tggcatggcc actagccccg ccgcgttatg cgaggttgcc aacgccgggg tcggccgagt
     3481 cgtcgtcgag agtcctacgg aggtcgcctt tctggccgcc gatttgcgtc gacctcaagc
     3541 cgtacaaatc cgcgtctcac cggacatcga ctgggcggcc aacgtcatcc agcgggcact
     3601 ggatcagccg atgctccatc tggtcggtct gcagtgtcat atcggttccc acgtgaccga
     3661 ggccccgctc tacggcgaag ccatccggca gatgatccca ctgatggccg agatccgcgc
     3721 ccgccatggg gtgatactga ccgagctgaa cattggtggc gggcagggtg tcccgcacgt
     3781 gtccggtgac ccggagttgg atctcgaagc gatgcgggac ttcgtcgagg attcgctcga
     3841 cgcgtcgtgt gccgccgaac gttttcctcg ccccactgtc gtggtcgagc ccggccgcgc
     3901 gatcagcgcg cgggccggtg tcacgctgta tcgggtcctg tgggtgcaat cccaacctgg
     3961 cggccgcacc atcgtggtgg tggacggcgg tttgagcgac tcccctcagg tcgcgcgccg
     4021 tgcgagctac accgtcgcgg tggccaaccg tcacccgatg acggcgacgc agccgatgac
     4081 ggtcgtcggg cggcagtgtg agtctggtga tgagatcgcc cgcgacgtgc cattgcccgc
     4141 cgatctacac gccggagacc tgcttgcggt ggcgtgcacg ggcgcttatc accacagcat
     4201 ggcgtcaacg tacaacatgg tgggccggcc accactggtc gcagtgaagg acgggcgcac
     4261 ccgcgaactc atccgccggg agacgatcgc cgacctatta tcgcgcgacc gcgtctataa
     4321 ggaacggccg ccgatgcggc gtgggcgcat cgagagcggc tgcgaagcgc ttcttcacgg
     4381 cgagtgatct gcgtcgggtg ttcgctcgtg acactggacg gtgcgtggtg ttgtccgcgg
     4441 tgacatcgac acccgtggcg acgcggccga cgatcgaagg gtcggcggaa cgcattccgg
     4501 catccatacc tattggaaac gaggatcgcg atgaatagcc ttgtcgggca gcggctgtgg
     4561 aaatggaagc tggtagcggg tctgttgacc attgtcttgg gcgcccttgt gttggtgtgg
     4621 cccgggccat cgatcctcgt cgccgccacc ctgtttggcg tctacctgtt gctgagcggc
     4681 atggtcgaac tgttcttggc cttcacgctg ccgagatcgg caggtacccg ggtgatgctg
     4741 ttcatcagcg gcgcactgtc attggtccta gccattctgt cgttccgcca cttcggtgac
     4801 ggctacgcgg tgctgctgct gtcgctgtgg attgggatcg ggttcatctt ccaaggtgtg
     4861 accggggtgg ccgtcggtat cggtgagagc gaactgcccg gccgcggctg gtacgtcgtc
     4921 gccggcatca tctcggtgat cgccggtctg gtggtgctgg tgtggccatt cgcgtcaatc
     4981 gccgtgctca ccctggccgc cggcatctgg ctcgtcatca tcggcatcac ccaaatcgtc
     5041 caggcagttc aaacccgcaa agccgccaag accgcacatg aagccctcga cgaagtatca
     5101 aaccggataa agacggccct cagtgattaa caacagattg gagacagcca tgaccacgag
     5161 ttctcgcgat gtcgacaata ggagcggaca ggagtcgccc tcaggcgcaa cggcaatgat
     5221 cgccgccggt gccctgatgt tgacgagcgc cgtactcacg tttcttatcg gtgtattcgc
     5281 ccttgccgct gacgatttgg tggtttccgg gcccggctac gagtacacct tccagatgac
     5341 cggttgggga tgggcgaaca tcctgacggg aatggtgttg gccggcgtcg ccattgccct
     5401 cttgtcgagc gcgcagtggg cgcgagctgc ggccatcgtc gtcacctgct tggccatcgt
     5461 ggtcaccttc ctctggatgc cgtattaccc cacgggctca attgtgctga tagccctgga
     5521 cgtcgtggtc atttggggcg tcgcaacgtg gaatacagca cgcaagacgg catgaaaccg
     5581 ccaccggcga aacccgaaaa gagccgccgg gcctgggccg tgtcacgcaa gcagttcgct
     5641 cttaacggga ccctcgctga ccgggtgatt tcggtcgccc acattattgg ccgcccggtc
     5701 cgcaacgtcg cgggtacacg aatcgggaga gtcagcgaca tcgtcgtgcg ctgggacgcg
     5761 gacaacgagc accccccggt cacaggggtt ttcgtcaagg tgcgcggtgg ccttgcgacc
     5821 gcgcaagtgg cggacgtgac gttgactcaa accgaaatcc gcctgcgatc cgatgcgcga
     5881 atgcagtggc ggccggtctg ggcggacgac gacgtagcgc ttgcccgtga cgttctcgac
     5941 cgccagctcg tcgacacgtc cggcgcacag gtcgtacggg cagccgacgc gta
//
LOCUS       DASRJA010000434        11438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1464394,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000434 DASRJA010000000
VERSION     DASRJA010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11438
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464394"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFC01_19070"
     CDS             <1..474
                     /locus_tag="VFC01_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81762.1"
                     /translation="MMGAITGTIVGMLFPLFCFGLGAFRAGYTPAILAMLYDFGYLAF
                     IGSLGCFCVMWMAFGLAIILDENIILPKWLGYYTVWQYVSELIAAPVWIAKSGPFAWN
                     GLMTFWLAMTLFVSWQVIVYVCIFRAIKNQPADELEHAWLGPDPASTDVGAATSA"
     gene            471..1070
                     /locus_tag="VFC01_19075"
     CDS             471..1070
                     /locus_tag="VFC01_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HZN81763.1"
                     /translation="MTEVSEEFSTATRNIEFRRHVPGDPSMWFFVIGDLLIFGVYFLG
                     YMYYRGENHVVFLQSQAKLNLNIGAVNTVVLLTSSLFVALGTAAARAGKTPDALRLFG
                     FALVCGAAFPLLKLFEYIPEITAGMTPGSNLFFMYYYVMTGLHLCHVLLGLVILCFVI
                     RNLRGPAKPRISFVETGATYWHMVDILWLILFALLYLMR"
     gene            1076..1354
                     /locus_tag="VFC01_19080"
     CDS             1076..1354
                     /locus_tag="VFC01_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HZN81764.1"
                     /translation="MTTYLRNPLTYVWALLTAVTVASWLTARDAGGAHQLNATVTVVV
                     LLIAAVKTQLVIWHFMEVRDAPTWLRATTSGWVVALFGLLMGLYFAAQ"
     gene            complement(1371..2489)
                     /locus_tag="VFC01_19085"
     CDS             complement(1371..2489)
                     /locus_tag="VFC01_19085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003917712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN81765.1"
                     /translation="MHNTINSRFGPKPLIQADYMTGLAAGADSFWVADHLNSVLPPSM
                     WKPKYVGAAKIIPKMDAHVEPWTMLGYLAGHNKMGRMTLGVAVTDTGRRNPAVTAQAV
                     VTLQHLTRGRAILGIGTGEREGNEPYGVEWSKPVGRFEEAMATIRALWDSNGELVSRD
                     SPFFPLHNATFALTPYKGKWPEIWVAAHGPRMIRAAGRYGDGWFPGTTRADEYGQQLN
                     DLRAAASDAGRDPMSITPAGIRFILTGRSQDEIDEILDSDLVKLFVLNSPAKDWERHG
                     AQHPMGPDFKGVQDLIPQLIDEESALKYAAMAPRGLVKELFAVGMPDEIVDQLSLFRD
                     NGMRCLVVGNMGALHPKLSKSAAAMTPYVKVLRRLKKL"
     gene            complement(2647..3072)
                     /locus_tag="VFC01_19090"
     CDS             complement(2647..3072)
                     /locus_tag="VFC01_19090"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN81766.1"
                     /translation="MFTGPAEDQLKIRERIDSYCDAVCRTALDDYLACWTEDGVRLGE
                     GGECRGTDELRAHWDGIWQVVSKMAFMAQIGAIEVTGDHARTRSYCLEILQFRVGGTR
                     RLIGTYDDKLRRVDGEWLFSERRYRIFLDESSPDAKDES"
     gene            complement(3177..3962)
                     /locus_tag="VFC01_19095"
     CDS             complement(3177..3962)
                     /locus_tag="VFC01_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN81767.1"
                     /translation="MNGSCDGKVALVTGTSRGLGKAIAQRLAAEGATVALTARTMEPD
                     PKYQGSLRETLDEIVGSGGAAIAVQADLSDAEERERLFREVVERVGAPDILVNNAAVT
                     FLRPLDEFPDRRVRLMMEMHVVAPLHLTQLAIPAMRERGSGWILNVTSVGGELPDGPP
                     FSDFDRTAGFGVYGTVKAALNRLTKSLAAELYDDGIAVNAAAPTNPVATPGAGTLDLA
                     KVDTEDIVLITRTALRLCTGDPKVLTGRIAHTQTFLREVGWLK"
     gene            complement(3979..5274)
                     /locus_tag="VFC01_19100"
     CDS             complement(3979..5274)
                     /locus_tag="VFC01_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN81768.1"
                     /translation="MAWDFSTDPQWADQLKWVEEFVRNECEPIDLIVKESHDLNDPVR
                     QALIPPLQKIVKKRGLWATHLGPHLGGPGYGQVKLALLNEILGRSECAPIVFGSQAPD
                     SGNSEILAHYGTPELKERYLEPLLDNRIISCFSMTEPQGGADPKVFTTTASQDGDHWV
                     INGEKWFSSFASMASFIIVMAMTDPDARPYERYSMFVVPAETAGINVLRDVGLGYQPP
                     GGGREGYVSYENVRVPADHMLGPRGGAFVVAQTRLGGGRIHHAMRTVGLVRRIYDMIC
                     ERAVSRYTQGGMLSDKQLVQEMVADSWMEIEAFRLLTLQTAWKIDQHNDYKAVRADIS
                     AVKAMMQKVLHDVSARALQVHGSLGTTHEMPFVQYLTESFVLGLADGPTEVHKVTLAR
                     LLLKDCKPAPDVFPSEHLLRLREAAEAKFAGRLAGIPRS"
     gene            complement(5286..6053)
                     /locus_tag="VFC01_19105"
     CDS             complement(5286..6053)
                     /locus_tag="VFC01_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN81769.1"
                     /translation="MTQTLILDRPQPGVIVLQLNRPNSLNAINEQMRDDLMQTFAMIG
                     ADASVNVVVLTGAGRGFCSGIDVRNFGGKPLEASDPAIARLRFQEAMAALPQAIWELP
                     QPVIAAVNGPCVGAGFALCLASDIRICSRAASFGNGAILLGLSGAEMGMSYHLPRIVG
                     TSVAADWMLTGRTVSADEADRRGLVSELVEPDQLIDRAVELASGIAELAPLGVRLTKR
                     ALQVNTDAAGPGPAMELENRNQVLTHATPEAEARRKR"
     gene            complement(6059..7234)
                     /locus_tag="VFC01_19110"
     CDS             complement(6059..7234)
                     /locus_tag="VFC01_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN81770.1"
                     /translation="MQLTFDSDVEEFRAEFAAFLDEHVPSEAETLERPKSVSHMPQWA
                     RDWQRLLFDNGWLLPAQPPEFGGRNATVLQQFVHLDELCRRRIYHSFNPQGVNIIAAS
                     LLSFGSDEQKHRWAVPVLRGEKTASLGMSEPSAGSDLASLRTRAELDGDHFVVNGQKV
                     WTSGAHDADFLLTFVRTDPDAPKHKGISALIIPTDTPGVVCRPFADMTGQENLDFNEV
                     FFTDARVPAENLVGPLNGGWGVANGSLGHERTMMWLGFADRIDNMIADFRPHTALERD
                     QYATTIMDYQALRAMGSAALARAARGEMDTASVSVLKLFGSEAERQAFENALTAAGPE
                     GLTHPSTSGPYAHMNLDHYFVSWFERYARSFGGTIAGGTSEIQRNIIATQVLGLPRR"
     gene            complement(7218..8216)
                     /locus_tag="VFC01_19115"
     CDS             complement(7218..8216)
                     /locus_tag="VFC01_19115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN81771.1"
                     /translation="MLLEFDADQRLWQETVRDAVAKQCPPSLVRGIAENGVDPAPLWQ
                     TYVDAGWTELTDPENAVELAIVLEELGRATDPTPYLATLTQFAPLAGDRFDPQQAGTA
                     VYGGVTAHRDSDGWVLNGTAHHVLDGDRAERLAVVTEAGVFVVDAAEVAARRSPVFDP
                     ILHIAEVTFAAVRVPDSDRVHVDAEKARHVALAGMAISTVGACQRILDLVLEHVKQRQ
                     QFGVAIGSFQAVQHKAVDMHVATERARALSYFAALTISADDPRRRLAAAMAKAAAGEC
                     QAVVFRHGLQLFGAMGFTWENDLQFALKRAKAGELLLGGAAEHRAIIAEEYRAADF"
     gene            8489..9574
                     /locus_tag="VFC01_19120"
     CDS             8489..9574
                     /locus_tag="VFC01_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN81772.1"
                     /translation="MQHPDGTQTPVIDASVHIFSKSNKDLRSFLREPFKSRGFPDYEM
                     DWYGAPGGEYAPKTEGPDRQYPGSDPDFVGGQLFNERGVDVAILHPMTRGIMPDRHLG
                     TAIAAAHNELMVSRWLEDNPYAEQFRGTIRVNPDDITGALREIAKYKDHPRVVQIAVP
                     LQSRELYGKPQYWPLWEAAVAANLPVAVHIEVGAGVQYAPTPSGVPRTYENYVSFMAL
                     NYLYHLMNLIVEGVFERLPTLKFVWADGAADLLTPFMWRMDCFGRPHLEQTPWAPRMP
                     SDYLPGHTYFVQGAVDGPGDTEFAGEWFGFTGKDDMVMYGSSYPHWQLNELKVPTAFS
                     AEQRDKLCWRNAAELYGIDVGAGVSAQ"
     gene            9604..10746
                     /locus_tag="VFC01_19125"
     CDS             9604..10746
                     /locus_tag="VFC01_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN81773.1"
                     /translation="MTVTVTDRKPAAEQIAVRCVDSDVHPVPKSGVLAQYIPEPWRSK
                     YFLPRKIGDQIYYDAPDYAHAYAMRTDTFPADGEFAGSDPDLAFKQLIMEAGADIAIL
                     EPAAYPARFPEVNHAMSCALNDWQANHWLDSHNNWHERWRGSICVAIEAPDDAAREID
                     RWAGHPYMGQILIKAEPRPAWGDPKYNPIWEAATKNDITVSCHLSRSHHEELPIPPVG
                     FPSYNHDFMVTYSLLAANQVMSMVFDGLFDRFPTLRIVLVEHAFTWILPLMWRMDAIY
                     EARKSWVDIKRKPSEYVKDHIKFTTQPLDYPEDKTELTRAFEWMECEKILLFSSDYPH
                     WTFDDPRWLVKHLPEHAREAVMFRNGIATYHLPETVPALEGQVRVF"
     gene            10748..11140
                     /locus_tag="VFC01_19130"
     CDS             10748..11140
                     /locus_tag="VFC01_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN81774.1"
                     /translation="MADTEKTPRLAQGREHVVATVDEIPPGTHKLVPIGRHGVGVYNV
                     NGTFYAIANYCPHEGGPLCSGRARGRTVVDDSVPGDAVMVRDLEFIYCPWHQWGFELA
                     TGTTAVKPEWSIRTYPVRVVANEVLVQA"
     gene            11137..>11438
                     /locus_tag="VFC01_19135"
     CDS             11137..>11438
                     /locus_tag="VFC01_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN81775.1"
                     /translation="MTTGEQIFEYKGGKVVYEILGESGDFIVLTPGGRFSKDIPGLRP
                     LAQALVDGGYRVVLWDRPNCGKSDVQFYGQSESHMRAETLHALITALGIGPCIIAGG"
BASE COUNT         2048 a         3742 c         3537 g         2111 t
ORIGIN      
        1 atgatgggtg cgatcaccgg cacgatcgtc gggatgcttt tcccgctgtt ctgctttggg
       61 ctgggcgcct tccgcgccgg ctacaccccg gcgatcctgg cgatgctcta cgacttcggc
      121 tacctggcgt tcatcggctc gttggggtgc ttctgcgtca tgtggatggc gtttggtctg
      181 gccatcatcc tcgatgagaa catcattctg ccgaaatggc tgggctacta caccgtctgg
      241 cagtacgtga gcgagctgat cgccgcaccg gtgtggatcg ccaagtcagg cccgttcgcg
      301 tggaacggtc tgatgacgtt ttggttggcg atgacgctgt tcgtgtcatg gcaagtcatc
      361 gtgtacgtgt gcatcttccg ggcgatcaag aatcagcccg ccgacgaact tgagcacgct
      421 tggctcgggc cggatcccgc gtcaaccgat gtcggggcgg cgaccagcgc atgacggaag
      481 tttcagaaga gttcagcacc gccacacgaa atatcgagtt tcggagacac gtgcccggtg
      541 atccgagcat gtggttcttc gtcatcggcg acctactcat cttcggggtc tacttcctcg
      601 gctacatgta ttaccgcggc gaaaaccatg tggtgtttct gcagagtcaa gcgaagttga
      661 acctcaacat cggcgccgtc aacaccgttg tgttgctgac cagttcgctc ttcgtcgcgc
      721 tgggtaccgc agcggcgcgt gccggaaaga ccccggatgc attgcggctg ttcggtttcg
      781 cactggtctg cggcgcggcc tttccgttgc tcaagctatt cgaatacatc ccggagatca
      841 ccgccggcat gacccccgga tccaacctgt tcttcatgta ctactacgtg atgacgggac
      901 tgcatctgtg ccacgtgctc ctcggcctgg tgatcctctg ctttgtcatc cgcaacctcc
      961 gcgggccagc gaaaccccga atctcgttcg tggaaaccgg tgccacctac tggcacatgg
     1021 tcgacatcct gtggctgatc ctgttcgcgc tgctgtactt aatgaggtga taggtgtgac
     1081 gacgtatctg cgcaacccgc tgacctacgt gtgggcactg ctcacggcgg tcacggtcgc
     1141 atcatggctg accgcccgcg acgctggcgg ggcacaccaa ctcaatgcca ctgtcaccgt
     1201 ggtcgtgctg ctaatcgcgg cggtgaaaac acaactagtg atctggcact tcatggaggt
     1261 ccgggacgcg cccacgtggc tcagggcaac gacgagcgga tgggtcgttg cactcttcgg
     1321 gctgctgatg ggtttgtact tcgccgctca gtaaacccgg ccgtgcgccg tcacagtttc
     1381 ttcagtcggc gtaggacctt cacgtacggc gtcatcgccg cggcgctctt cgagagcttg
     1441 gggtgtagtg cacccatgtt gccgacgacg aggcatcgca tgccgttgtc ccggaacagc
     1501 gacaattggt cgacgatttc gtccggcatc ccgaccgcga acaattcctt caccagcccc
     1561 cggggggcca tcgccgcgta cttgagagca ctttcctcgt cgatcaactg tgggatgagg
     1621 tcctggaccc ccttgaagtc cgggcccatc ggatgttggg cgccgtgcct ctcccaatcc
     1681 ttcgcgggcg agttgagtac aaacagcttc accaagtcgg agtcgagaat ctcgtcgatc
     1741 tcgtcttggg atcgtccggt gaggatgaac cggataccgg ccggcgtgat cgacatgggg
     1801 tcccgcccag cgtccgaggc ggctgcgcgc agatcgttga gttgctgtcc gtattcgtcc
     1861 gcccgggtag tccccggaaa ccagccgtcc ccgtacctgc ccgcagcacg aatcatccgt
     1921 gggccgtggg cggcgaccca gatctcgggc cacttgcctt tgtacggagt caaggcgaac
     1981 gtggcgttgt gcaacgggaa aaatggtgaa tcccggctga cgagttcacc gttcgagtcc
     2041 cacagcgcac ggattgtggc catcgcctct tcgaagcggc cgaccggttt ggaccactcg
     2101 actccatagg gctcgttgcc ctcccgctcg ccggtcccga tcccgaggat ggctcgccca
     2161 cgggtgaggt gctgcagtgt caccacggcc tgcgcagtga cggcgggatt tcgccggccc
     2221 gtatcggtaa ccgccactcc cagcgtcatc cgacccatct tgttgtgccc ggctagataa
     2281 cccagcatcg tccaaggctc gacgtgcgca tccatcttgg ggatgatctt ggctgcgcca
     2341 acgtatttcg gcttccacat cgatggcggc agcaccgagt tgagatggtc ggccacccaa
     2401 aacgagtcgg cacccgcggc caggccggtc atgtaatcgg cctgaatcaa cggcttcggc
     2461 ccaaaccttg aattgatcgt attgtgcatg acgccgatgc ggagaccggt caccatgacc
     2521 tccctcgatt ggttgccgat ggcaaaagtc gaatgtcggc gttctcaacc ggccgcgcgc
     2581 tactcggact gaggagtgaa ggtgaggtgc agttcggaga ggcgtaatcg atggtgtcga
     2641 aatcggtcat gactcgtcct ttgcgtcggg cgaactctcg tcgaggaaga ttcggtagcg
     2701 gcgttcgctg aacagccact cgccgtcgac gcgccgtaat ttgtcgtcgt acgttccgat
     2761 caagcggcgc gtgccgccga ctcggaactg gagtatctcc aggcaatatg accgggtacg
     2821 tgcgtggtca ccggtgacct cgatggcacc gatctgcgcc atgaacgcca tcttcgacac
     2881 aacctgccag atgccgtccc aatgggcgcg caactcgtct gttccgcgac attcaccgcc
     2941 ctccccgagc cggacgccat cctcggtcca gcaggccaag tagtcgtcaa gcgcggtccg
     3001 acagacggcg tcgcaatagc tgtcgatccg ttcccggatc ttgagctggt cttctgcggg
     3061 tccagtgaac actcgtgaac tcctccctca cccgaggttg tgggaaaact gcggcgaacg
     3121 tgggttaccc gcacgcaaaa tgctgcgccg gcgtgacaca atcccacact cgtcacttac
     3181 ttcaaccagc cgacttcacg caagaacgtt tgcgtgtggg cgatgcgacc ggtcagcacc
     3241 ttggggtcgc cggtgcacag ccgcaacgcg gtccgggtga tcaacacgat gtcttcggtg
     3301 tcgaccttgg ccagatcaag tgtgcccgcg ccaggggtgg cgaccggatt ggtgggcgcc
     3361 gcggcgttga ccgcaatacc gtcgtcgtac agctcggccg caaggctttt cgtcagccgg
     3421 ttcagcgccg ccttgaccgt gccgtagacg ccgaaccctg cggtgcgatc gaagtcggag
     3481 aacggcggtc cgtcaggcag ctcgccaccg accgacgtga cgttgaggat ccatccgcta
     3541 ccgcgttcgc gcatcgcggg tattgcgagt tgggtcaggt gcagcggcgc cacgacatgc
     3601 atctccatca tcagccgcac ccggcgatcc ggaaactcat cgagcggacg caggaacgtg
     3661 actgcggcgt tgttgaccag aatgtccggc gccccaacgc gttccaccac ctcgcgaaac
     3721 aaccgctcgc gctcctcggc gtccgacagg tcggcctgga ccgcgatcgc agcaccgccg
     3781 gagccgacga tctcatccag cgtctcccgc agggagcctt gatatttggg gtcgggctcc
     3841 atggtgcggg cggtcagcgc aacggtcgcg ccttcagccg ccagccgctg cgcgatcgcc
     3901 ttcccgagtc cacggctggt gccggtcacc aacgccacct tgccgtcaca ggatccgttc
     3961 acgtgtcacc ttcctgggtc acgagcgggg tatcccagcg agcctgccgg cgaacttcgc
     4021 ctcggccgcc tcacgcaacc gaagcaggtg ctccgacgga aacacgtcgg gtgcgggctt
     4081 gcagtccttg agcagcagac gcgccagcgt gaccttgtgc acctcggtgg gtccgtcggc
     4141 cagtccgagc acgaacgatt cggtcagata ctgcacgaac ggcatttcgt gtgtcgtacc
     4201 aagcgacccg tgtacctgca atgcgcgggc cgacacgtcg tgcagcacct tctgcatcat
     4261 cgccttgacc gctgagatgt cggcgcgcac ggccttgtag tcgttgtgct ggtcgatctt
     4321 ccacgccgtc tgcagagtca gcagccggaa cgcctcgatc tccatccacg agtcggcgac
     4381 catctcctgc accaactgct tgtccgacag catcccgccc tgggtgtacc gcgacaccgc
     4441 ccgctcgcag atcatgtcgt agatgcgccg gaccagcccg accgttcgca ttgcgtggtg
     4501 aattcgcccg ccgcccaacc gcgtctgggc gacaacgaac gcaccgccgc gcgggccgag
     4561 catgtgatcg gctggcaccc ggacgttctc gtagctcaca tagccctcgc gaccgccgcc
     4621 ggggggctgg taacccagcc cgacatcgcg cagcacgttg atgccggccg tttcggccgg
     4681 cacgacgaac atcgaatacc gttcatacgg tcgcgcatcc gggtctgtca tcgccatcac
     4741 gatgatgaac gacgccatcg aggcgaacga cgagaaccac ttctcgccgt tgatgaccca
     4801 gtgatcaccg tcctggctcg cggtggtggt gaacaccttg ggatccgcac cgccctgcgg
     4861 ctccgtcatc gaaaagcagg agatgatccg gttgtccagc agcggctcca gataccgttc
     4921 cttcaactcc ggcgtgccgt aatgcgcaag gatctcgctg ttgcccgaat caggcgcctg
     4981 cgagccgaag acgatcggtg cgcattcgga ccggcccaga atctcgttga gcagcgccag
     5041 tttcacctgc ccatagccgg ggccacccaa gtgcgggccg agatgggtgg cccacaagcc
     5101 gcgcttcttg acgatcttct gcaacggcgg aatcaacgcc tggcgcaccg gatcgttcag
     5161 gtcatgggat tccttgacga tcaggtcaat cggctcacat tcgttccgca cgaattcttc
     5221 gacccacttg agttggtccg cccactgcgg gtcggtcgag aaatcccacg ccatggtgtc
     5281 tcctgtcagc gcttacgtcg agcctcggcc tcaggggtcg cgtgagtgag cacctgattc
     5341 cggttttcca gttccatagc cggtcctggg ccggccgcat cggtgttcac ctgcagggct
     5401 cgcttggtca gccgcactcc cagtggcgct aattccgcta tcccagaagc caattccact
     5461 gcgcggtcga tgagttgatc gggttcgacc aattcgctga ccaacccccg gcggtcggcc
     5521 tcgtcggcgg acaccgtacg gccggtcagc atccaatcgg cggcgacgct tgtcccgacg
     5581 atgcggggca ggtgatagct catgcccatc tccgcgccgg acagccccag cagaatcgcg
     5641 ccgttgccga agctcgctgc cctcgagcag attcggatgt cagacgccag acatagcgcg
     5701 aaccccgcac caacgcatgg tccgttgacc gcggcgatca ccggctgcgg aagttcccaa
     5761 atcgcttgcg gcagcgccgc catcgcctcc tggaaccgta accgcgcgat cgccggatcg
     5821 gacgcctcga gtggcttacc cccgaagttg cgcacgtcga tcccggaaca gaagccgcgt
     5881 cctgcgcccg tcagcacgac gacgttcacc gaggcatcag caccgatcat ggcgaacgtt
     5941 tgcatgagat cgtcgcgcat ttgctcgttg atcgcgttga gcgaatttgg tcggttcagt
     6001 tgcaggacaa tgacaccggg ctgcggccgg tcgaggatga gggtctgcgt caccggactc
     6061 agcgccgcgg caagccgagc acctgggtcg cgatgatgtt gcgctggatc tcggaagtgc
     6121 cgcccgcgat cgtgccgccg aagcttcgcg catagcgctc gaaccaactg acgaagtagt
     6181 ggtcgaggtt catgtgcgca taaggtcccg acgtcgacgg gtgcgtgagt ccttcgggcc
     6241 ctgcggcggt cagcgcattc tcgaacgcct gccgttccgc ctccgaaccg aacaacttca
     6301 gcaccgacac cgatgcggtg tccatctctc cgcgcgccgc gcgcgccagc gccgccgacc
     6361 ccatggcccg cagcgcctga tagtccatga tcgtggtggc gtactggtcg cgttccagcg
     6421 cggtgtgggg ccggaagtcg gcgatcatgt tgtcgatccg gtcggcgaag ccgagccaca
     6481 tcatcgtgcg ctcgtggccg agcgacccgt tcgccactcc ccaaccgccg ttgagcgggc
     6541 cgacgaggtt ctccgcgggc acccgcgcgt cggtgaagaa gacctcgttg aagtcgagat
     6601 tctcctggcc ggtcatgtcg gcgaacggcc ggcacaccac tccaggggtg tcggtgggaa
     6661 tgatcagcgc gctgatcccc ttgtgcttgg gcgcatccgg gtccgtccgt acgaacgtca
     6721 gcaggaagtc ggcgtcatgc gcgcccgacg tccacacctt ctggccgttg acgacgaaat
     6781 gatcaccgtc caactcagcc cgcgtgcgca gtgacgccag gtcggagccc gcgctgggct
     6841 cgctcatgcc gagagacgcg gtcttttcac cacgcagcac cggcacggcc cagcggtgct
     6901 tctgttcgtc ggaaccgaac gaaagcagcg acgccgcaat gatattgacg ccctgtgggt
     6961 tgaagctgtg atagatccgc cgccggcaca gctcgtcgag gtgcacaaac tgctgcagca
     7021 cggtggcgtt gcgcccgccg aactccggcg gctgcgcagg cagcagccag ccgttgtcga
     7081 acagcaaccg ctgccagtcg cgcgcccatt gcggcatgtg cgacaccgac ttcggccgct
     7141 cgagcgtctc ggcttccgac ggaacgtgct cgtcgaggaa cgccgcgaac tcggcgcgga
     7201 actcctcgac gtcggaatca aaagtcagct gcacgatatt cctccgcgat gatcgcgcga
     7261 tgctctgccg cgccgccgag cagcagttca ccggccttgg cccgcttgag tgcgaattgc
     7321 aggtcgttct cccaggtgaa tcccatggcg ccgaacagct gcaggccgtg ccggaacacc
     7381 accgcttggc actcccctgc tgcggccttt gccatcgccg cagcaagccg gcggcgggga
     7441 tcgtcggccg aaatggtcag tgcggcaaag taagacaacg cacgggcccg ttcggtggca
     7501 acgtgcatgt cgacggcctt gtgctgaacg gcctgaaacg acccgatcgc gacgccgaac
     7561 tgctgacgct gcttcacgtg ctcgagcacc aaatcgagga tccgctgaca cgccccgact
     7621 gtcgagatgg ccattcccgc cagcgccacg tgccgggcct tctcggcatc gacgtgcacc
     7681 cggtcgctgt ctggaacgcg cacagcggcg aacgtcacct cggcgatgtg caggatggga
     7741 tcgaacaccg gactgcgtcg cgcggccact tcggctgcgt cgacgacgaa tacccctgcc
     7801 tcggtgacga ccgccagccg ctcggcgcgg tcaccgtcga gcacgtgatg cgccgtgccg
     7861 ttgagcaccc agccgtcgga gtcccggtgc gccgtcaccc cgccgtagac cgccgtcccc
     7921 gcctgctgcg ggtcgaagcg gtccccggcc agcggcgcga actgcgtcag cgtcgccaga
     7981 tagggcgtgg gatcggtggc gcggccgagc tcctcgagaa cgatcgccag ctcgacggcg
     8041 ttctccgggt cggtcagctc cgtccagccc gcgtcgacgt aggtctgcca cagcggggcc
     8101 gggtcgacgc cgttctcggc gatgccgcgc accagcgacg gcgggcactg cttggccacg
     8161 gcgtcgcgca cggtctcctg ccacagccgc tgatcggcat cgaactccag taacacctga
     8221 gcctcccggg gctggtcgcg tcagaacgag aataacattc tcgttagatg ctgtaaccgt
     8281 tctctctatt cgactaccac gtttcgccgt acgcccacca tgcgactgac cagcacgcac
     8341 cctgacctgc gcgaaggtaa gcgattcgct gagaactaga ttcttgctat tgaagaacaa
     8401 cgctttacac taggagtatc cggcgcgctt cgcgcagcgc tcgaccgcgc tcatgtcggc
     8461 cggatgacat cggaggacgg ctttggcgat gcaacacccc gacgggaccc agacgcccgt
     8521 catcgatgcc agcgtgcaca tcttcagtaa gtcgaacaag gacctgcgca gcttccttcg
     8581 tgagccgttc aagagccgcg gtttcccgga ctacgagatg gattggtacg gcgcgcccgg
     8641 cggtgaatac gccccgaaga ccgaggggcc cgatcgccaa taccccggtt cggatcccga
     8701 cttcgtcggc ggccagttgt tcaacgaacg cggcgtcgac gtggcgatcc tgcacccgat
     8761 gaccaggggc atcatgcccg accgccatct cggcacggca atcgccgctg cgcacaacga
     8821 gttgatggtg tctcgctggc tcgaagacaa cccgtatgcc gagcaattcc gcggcaccat
     8881 ccgggtgaac cccgacgaca tcaccggcgc ccttcgtgag atcgccaagt acaaggacca
     8941 cccgcgcgta gtgcagatcg cggtcccgct gcaatcgcgc gagttgtacg gcaagccgca
     9001 gtactggccg ctgtgggaag cggcggtcgc ggcgaaccta ccggtcgccg tgcatatcga
     9061 ggtgggtgcg ggtgtgcagt acgcaccgac cccgtcgggc gtgccgagga cctacgagaa
     9121 ctacgtcagc ttcatggcgc tgaactacct gtaccacttg atgaacctga tcgtcgaagg
     9181 ggttttcgaa aggctgccca cgctgaaatt cgtttgggcg gacggagccg ccgacctgct
     9241 gacgccattc atgtggcgga tggactgctt cgggcgcccc cacctcgagc agacaccgtg
     9301 ggcgccgcgg atgcccagtg actacctgcc cggccacacc tacttcgtcc agggcgcggt
     9361 ggacgggccg ggcgataccg aattcgcagg cgagtggttc ggcttcaccg gcaaggacga
     9421 catggtgatg tatgggtcga gttatccgca ctggcagctc aacgagttga aggtgcctac
     9481 cgccttctcc gccgaacaac gcgacaagct ctgctggcga aatgccgcgg aactgtacgg
     9541 catagacgtc ggggccggcg tcagcgcaca gtaagagaag acttcccgag caaggagacc
     9601 acgatgacgg tcacagtgac agatcggaag cccgcagccg aacagatcgc tgttcgctgc
     9661 gtcgactccg atgtgcatcc ggtgcccaag agcggcgtgc tggcgcagta catcccggag
     9721 ccgtggcgca gcaagtactt cctgccgcgc aagatcggcg accagatcta ctacgacgcg
     9781 ccggactacg cgcacgcgta cgcgatgcgg acggacacct tcccggccga cggcgaattc
     9841 gcgggcagcg atccggatct ggcgttcaag cagttgatca tggaggcagg cgccgacatc
     9901 gcgatcctcg aacctgccgc ctacccggcg cgcttccccg aggtgaacca cgcgatgagc
     9961 tgcgcgctca acgactggca ggccaatcac tggctggaca gccacaacaa ctggcatgag
    10021 cgctggcgcg gctcgatctg cgttgcgatc gaggcgccgg acgacgccgc ccgcgagatc
    10081 gaccggtggg ccgggcatcc ctacatgggc cagatcctga tcaaggccga gccacggccc
    10141 gcgtggggcg atcccaagta caacccgatc tgggaggcag ccaccaagaa cgacatcacg
    10201 gtgagctgcc acctgtcgcg cagccaccac gaggagctac cgattccgcc ggtcgggttc
    10261 ccaagctaca accacgattt catggtcacg tactcgctgc tggccgcgaa tcaggtgatg
    10321 agcatggttt tcgacgggct cttcgatcgt tttccgacgt tgcgaatcgt gttggtggag
    10381 cacgcattca cgtggattct tccgctgatg tggcggatgg acgccatcta cgaagcccgc
    10441 aagtcgtggg tcgacatcaa gcgcaagccg tcggagtacg tcaaggacca catcaagttc
    10501 accactcagc cgctggatta ccccgaggac aagacggaac taacccgtgc gttcgagtgg
    10561 atggagtgcg aaaagatcct gctgttcagc tcggactatc cgcactggac gttcgacgac
    10621 ccgcggtggt tggtcaagca tctgcccgag catgcaagag aggccgtgat gttccgcaac
    10681 ggcatcgcga cctatcacct gccagagacg gtgcctgccc tcgagggtca ggtccgggtg
    10741 ttctgaattg gctgacacgg agaaaacgcc tcggctggcg caggggcgtg agcatgtggt
    10801 cgccacggtc gacgagatcc cgcctggcac acacaaactc gtaccgatcg ggcgccacgg
    10861 ggtcggggtc tacaacgtca acggcacgtt ctacgcgatt gccaactatt gcccgcacga
    10921 gggtggtccg ttgtgctcag gccgtgcgcg tggtcgcacc gtcgtggacg acagcgtgcc
    10981 cggcgacgcg gtgatggtgc gcgacctcga gttcatctac tgcccgtggc accaatgggg
    11041 tttcgagctc gcgacgggca ccaccgcggt caaacccgag tggagcattc gtacttatcc
    11101 agtccgtgtg gtcgctaacg aggtgttggt gcaggcatga caactggtga gcagatcttc
    11161 gagtacaaag gtggcaaggt cgtctacgag atcctcggtg aatcgggcga tttcatcgtc
    11221 ctgaccccag gtgggcgatt cagcaaggac atccccggtt tgcggccgct ggcacaggca
    11281 ctggtcgacg gtggctaccg ggtcgtgttg tgggaccggc ccaactgcgg caagtccgac
    11341 gtgcagttct acggccagtc cgagtcgcat atgcgggcag agacgctgca cgccttgatc
    11401 actgctctgg gcatcgggcc gtgcatcatc gccggcgg
//
LOCUS       DASRJA010000435         3569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1189131,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000435 DASRJA010000000
VERSION     DASRJA010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189131"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFC01_19140"
     CDS             complement(<1..192)
                     /locus_tag="VFC01_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN81776.1"
                     /translation="MTANYPTKCWYVAATSDELDDAPLGRRLLDRDVVLWRSRDGQAV
                     AFDDGCAHRGFPLSDGHVDG"
     gene            complement(189..1190)
                     /locus_tag="VFC01_19145"
     CDS             complement(189..1190)
                     /locus_tag="VFC01_19145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HZN81777.1"
                     /translation="MTFTQEAPAVASAAKVHEFTTDLVVHRRHAAADGVVALDLVDPD
                     GNDLPAWEPGAHIDLLLDEGLVRQYSLCGDPLDAKTWRVGVLLDPQSRGGSRHVHDKL
                     VEGAGVRVRGPRNHFALVDSPRYLFIAGGIGITPIIAMIDSAQQAGSDWRLIYLGRQR
                     TTMAFAEVLADSYGDRVTLWPHDEKGKFDLEAALAESVDQTLVYCCGPEQLLSAAEQH
                     CAHWPEGSLHIERFAAKAAAEPSVEALDTFQVVCQRSGVTVEVSEGTTVLEALEDADI
                     PIMSSCLEGICGTCEATVLEGTPDHRDSMLTEAERATGNKLLTCVSRSCSEKLVLDL"
     gene            complement(1187..2614)
                     /locus_tag="VFC01_19150"
     CDS             complement(1187..2614)
                     /locus_tag="VFC01_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZN81778.1"
                     /translation="MHRLSALDAQFLAAEAGNAGSQYCGVAIYQTGEEQPITAATMRE
                     RLAQRISHCPPLRWKLVTVPFGLDRPMFVDSEVNLEDHVSETTLAAPADDATLATEVA
                     DILSLRLDRDKPLWKLQVIHGLPGRTAVVMTLHHAAVDGIAASEIFAAILDGRVDEAS
                     APCRDQAEVIPSQTALAARGIASIPLRRVRALRSAPATLAHLDQVPVVRSLPGVHTLS
                     QIVRGDLSAKRLNAPRTRFNAKLSATRSVAFGTVSLNDVKAVKNALGITVNDVVIALC
                     AGALRRRLTSTRDLPTDPLVAYIPVSTRLPDAKDRYGNAISSIIAPIPTHLAPARERL
                     EFAHETFKRAKQRAREAPPTLLSDVNEPIPLPVFGVAARGLMELVSSRFVRPPVNLII
                     SNVPGSPLKLTCHGAPLLAHYPMSLVFHGFTLNITVVSYNQGLDIGIVGDARTLPDAW
                     DLMDDFRLELAELVALVTTEGSSDQ"
     gene            complement(2614..>3569)
                     /locus_tag="VFC01_19155"
     CDS             complement(2614..>3569)
                     /locus_tag="VFC01_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007170545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN81779.1"
                     /translation="PSELAPATSRVLARLVPEYLDPAAVRVVEGDAAVTQDLLAQGFD
                     HALFTGGTEVGKKIMAAAAPTLTPVTLELGGKSPVIVAADADLDVAARRIAWVKLLNS
                     GQTCIAPDYVLAERSIADELVDKIVSNVREFRSGESDPSLRIVNQRQFDRLVSLISAT
                     DGTIVTGGEFDSATLQIEPTVIVDPSPTDAVMADEIFGPILPVVTVDSLDSAVDFVND
                     KPKPLALYLFTQSRRVARTLIDRIPSGGAVVNHVAMHCMVPQLPFGGVGASGMGAYHG
                     RWGFEAMSHRRAVLAKRARPDLKMMYPPYSDRAIKLMRKVF"
BASE COUNT          671 a         1129 c         1139 g          630 t
ORIGIN      
        1 gccgtcaacg tggccgtctg acagcgggaa gccgcgatgc gcgcagccgt cgtcgaaagc
       61 caccgcctgg ccgtcacgcg atcgccacag cacgacgtca cggtcgagca accggcggcc
      121 cagcggagca tcatcaagct cgtcgctcgt cgccgccaca taccagcatt tggtggggta
      181 gttcgcggtc ataggtccaa caccaacttc tcgctgcagg aacgggatac acatgtgagg
      241 agcttgttgc cggttgctcg ttcggcttcg gtcagcatgg aatcgcgatg atccggcgtg
      301 ccctccaaga cggtcgcttc gcaggtaccg cagatccctt ccagacatga cgacatgatc
      361 gggatgtccg cgtcttccaa tgcctcaagg accgtcgtgc cctcggacac ctccaccgtc
      421 acaccggagc gctggcacac cacttggaag gtgtccaacg cctcaacgct gggttcggca
      481 gccgccttgg ccgcgaaccg ctcgatgtgc agactgccct ccggccagtg cgcgcagtgc
      541 tgctcggcgg cggacagcag ttgttcgggc ccgcagcagt acacaagtgt ctgatctacc
      601 gactccgcga gcgcggcttc gagatcgaac ttccccttct catcgtgcgg ccacagcgtt
      661 acgcggtcgc catacgaatc ggccagcact tccgcaaacg ccatcgtcgt ccgttgccgt
      721 ccgaggtaga tcagtctcca gtcgctaccg gcctgctgcg cggaatcgat catcgcgatg
      781 atcggagtta tcccgatacc accggcgatg aacaggtatc gcggggagtc gacgagcgca
      841 aagtggttgc gaggtccacg aactcgcacg ccggcacctt caacgagttt gtcgtggacg
      901 tggcgtgaac cgccgcggct ctgggggtcc agcagaacgc cgacccgcca ggtcttggca
      961 tccagtggat caccgcagag ggaatactgg cggactaagc cctcgtcgag caggaggtcg
     1021 atgtgggccc cgggctccca ggccggcagg tcgttgccat ccgggtccac caaatccagc
     1081 gccaccacgc cgtccgccgc cgcgtgtcgc cgatgcacga caaggtcagt cgtgaactcg
     1141 tgcaccttcg cggcggatgc cacagccggg gcttcttgag tgaaagtcat tggtcagagc
     1201 tcccttcagt ggtaaccaga gcgaccagct cggccagctc aagccgaaag tcgtccatca
     1261 aatcccatgc atcgggcaat gttcgagcgt caccgacgat cccgatgtcg agtccttggt
     1321 tgtaactgac gaccgtgatg ttgagcgtga aaccgtgaaa tacgagactc atcgggtagt
     1381 gcgccaggag tggggcgccg tggcaggtca acttgagagg ggaccccggt acgttcgaga
     1441 tgatcaagtt caccgggggg cgcacgaacc gcgaagagac cagttccatc aggccacgag
     1501 cggcaacgcc gaacacgggt aaggggattg gttcgtttac atcgctcagg agtgttggcg
     1561 gcgcttcgcg ggcgcgctgc ttcgcacgct tgaatgtctc gtgtgcgaat tcgaggcgct
     1621 cccgagccgg ggccagatgg gtcgggatcg gcgcgatgat cgatgagatc gcgttcccgt
     1681 agcggtcttt cgcgtcgggc aacctggtcg acaccggaat gtaggcgacc agggggtctg
     1741 tcggcagatc gcgggtcgag gtcaacctgc ggcgcagcgc acccgcacac agcgcaatca
     1801 cgacgtcgtt gacggtaatg cccagggcgt tcttcaccgc tttgacatcg ttcaatgaga
     1861 cggtgccgaa ggccactgag cgggtcgccg aaagctttgc gttgaaacgt gttcggggtg
     1921 cgttgagccg cttggcgctc aggtcgcccc tgacgatctg cgaaagggta tgcaccccag
     1981 gtagtgaccg cacgaccggg acttggtcca gatgcgccag cgtcgcaggg gcagaacgca
     2041 gcgcgcggac ccgcctcagc ggaatggatg caataccgcg cgccgccaga gcggtctggc
     2101 tgggaatcac ttcagcttga tcccggcatg gcgcagaggc ctcgtccaca cgcccgtcga
     2161 gaatggcagc gaagatctcg ctcgccgcga tgccgtcgac cgcggcgtga tgcaacgtca
     2221 tgacgacggc ggtgcgcccc ggcaagccgt ggatcacctg cagcttccac agcggcttgt
     2281 cgcggtctaa ccgcaacgac aggatgtcgg cgacctcggt ggccagcgtc gcgtcatcgg
     2341 ccggcgcggc gagggtggtc tcgctgacat gatcttcgag gtttacctcg ctgtcgacga
     2401 acatcggtcg gtcaagtccg aagggcaccg tcaccagttt ccagcgcaac ggtgggcagt
     2461 ggctgattcg ctgcgcgagc cgttcgcgca ttgtcgccgc ggtgatcggc tgctcttcgc
     2521 cggtctgata gatcgcgacc ccgcagtact gtgagccggc gttcccagcc tcagccgcga
     2581 gaaactgagc atccaatgcc gaaaggcgat gcatcagaag accttccgca tgagcttgat
     2641 ggcccggtcg ctgtaaggcg ggtacatcat cttcaaatcg ggtcttgccc gcttggcaag
     2701 gaccgcccgg cgatggctca tcgcttcgaa gccccaccgc ccgtggtagg cgcccatccc
     2761 gctggcgccg acgccgccga acggcagctg aggaaccatg caatgcatcg cgacatggtt
     2821 gaccactgct ccgcccgacg ggattcggtc gatgagcgta cgtgcgaccc gtcgcgactg
     2881 ggtaaacaga tacaacgcca acggtttcgg cttgtcgttg acaaagtcca cagccgagtc
     2941 gagggagtca acagtcacta cgggcaggat cgggccgaag atctcgtcgg ccatgaccgc
     3001 gtcggtcggc gacgggtcga cgattacggt gggttcgatc tgcagcgtgg cgctgtcgaa
     3061 ttcgccgcca gtgacgatgg tgccgtcagt tgcgctgatc agggagacaa gacgatcgaa
     3121 ctggcgctga ttgacgatcc gcaatgacgg atcgctttca ccggagcgga attcgcgcac
     3181 attcgagaca atcttgtcga caagctcatc ggctatcgat cgctcagcca acacgtagtc
     3241 cggtgcgatg cacgtctgac cggagttgag cagtttgacc cacgcgatgc gccgcgcggc
     3301 gacgtcaagg tcggcgtcgg cggccacgat taccggactc ttgccaccaa gttcgagcgt
     3361 gaccggcgtc aatgttgggg cggcggcggc catgatcttc ttgccgacct cggtgccacc
     3421 ggtgaacaag gcgtgatcga aaccctgggc cagcagatcc tgcgtgaccg ccgcgtcgcc
     3481 ctcgacaacc cgcaccgccg ccggatccag atactccggc actagccgcg ccaaaacgcg
     3541 cgaggtcgcc ggcgccaatt cggacggtt
//
LOCUS       DASRJA010000436         8818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2592745,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000436 DASRJA010000000
VERSION     DASRJA010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2592745"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VFC01_19160"
     CDS             complement(<1..915)
                     /locus_tag="VFC01_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZN81780.1"
                     /translation="MIAAQQVVDTALAEAARLGKADETIVLVTDRADAALRWAGNSMT
                     TNGESVSRTTTVISIVRQGDTAHVGSVRSSDVDPAVIAAMVAESQQTAVSAPAARDGA
                     PPLPGTEVPADWDAPVPSTGARVFSGLAAPLAHGFQGVDQLYGYARHIVETTFVATST
                     GLRRRYTQPTGSVEINAKRDGASAWAGISTPDFVDVPIDSLLEQLSTRLGWAQRTVEL
                     SAGRYETIMPPSTVADMMIYLAWSMDGRGAQEGRTALSAPGGGTRVGEKLTELPLTLY
                     SDSAATGLECTPFVAVPNSSERVSVFDNG"
     gene            complement(919..2436)
                     /locus_tag="VFC01_19165"
     CDS             complement(919..2436)
                     /locus_tag="VFC01_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZN81781.1"
                     /translation="MTTSPRLDADFLALPRHQLADAALSTATAAGASYADLRIHCITT
                     EIVQLRDGELETSVVNREIGLAVRVIVDGTWGFASHAELDPEVAAKTARRAVEVATTL
                     APLNAERIELAAEPVYSDVSWVSDYLVDPFTVGVADKIAVLGEYSGRLLAADGVDHVS
                     AGLHAAKEQTFYADTFGSSITQQRVRVQPTFEAVTVDAVAAAFETMRTLAPPTARGWE
                     YAAGDDVWNWTDELAQLPSLLAEKAKAPSVSAGPTDLVIDPSNLWLTIHESIGHATEY
                     DRAIGYEAAYAGTSFATPDKLGTMRYGSSVMNVTADRTVEYGLATVGFDDEGVQAQRW
                     DLVREGVFVGYQLDRVFAPRLGVSRSNGCSYADSPHHVPIQRMANVSLQPSADDVTTQ
                     DLIARVEDGIYIVGDKSWSIDMQRYNFQFTGQRFFRIRDGRLDGQLRDVAYQATTTDF
                     WGALEVVGGPSTWRLGGAFNCGKAQPGQVAAVSHGCPSALFRGINVLNTRTEAGR"
     gene            complement(2599..5760)
                     /locus_tag="VFC01_19170"
     CDS             complement(2599..5760)
                     /locus_tag="VFC01_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN81782.1"
                     /translation="MTSAPTVCESCGTELRGGARFCDACGAPITASREPAEYKQVTVL
                     FADVVRSMNIAVAVGPERLREIMAGLVDHATAVVERYGGTVQSFTGDGIMALFGAPVA
                     MEDHALRACRAALDIQRDVQRQAAEVHERDDITLQLRIGLNSGQVIAGEIGSTAAGYT
                     AIGDQVGMAQRMESVAPPGGVILSTSTARLVESAAALGEPQLVQIKGRADPVPARQLV
                     GIIARRGEPRRWQSTLVGREPETHRIAQAMEQVLAGRGQVVGVVGAPGIGKSRLVSEA
                     AALARNRGADVHVTYCEAHARDIAFHAVANLLKADYAVAGLSDAAARQQVRSRFVDAD
                     TTDLMLLDDLLGIADPTAALPDIDPDARRRRLNALITAAHLERKNPTVYVIEDAHWID
                     EVSESMLADFLTAIKQSHSMMIFTYRPEYRGALTEVAESQRISLAPLDDSQSITLLTE
                     LLGSDPSVTQIGARVTERAAGNPFFVEEIVRDLAERDVLVGSHGNYVCRGDVADDSVP
                     ATLQATIAARIDRLGPAAKRTVRTAAVVGSRFTADLLVSLGVDPALKALTEAELIDEV
                     TSGAEREYAFRHPLFRTVAYESQLKADRADLHRRLAIAIEKREAHAVDASAALIAEHL
                     EAAVDLGGAYAWHMRAAGWLATRDVAGARISWRRAQAVADQMPDGDLNVTAMRIAPRT
                     LLCATAFRVGGSVADTGFAELQDLTDASGDKRSLAMGMSGFVMALTVHSRHRESARLA
                     SECMELFESIGDPALTVGLAFPLVLAKYEAGEMNEALRIAERAIDLAGGDPTKGNLVF
                     GSPLALLLAIRGTLRYRIGLSPGRDDADRAVAMARAYDPSSLVFAVMYKYLGITTGAF
                     LPDEAAVHDTADALQLAERSSDDFALAAARFTRGITLLYREGEDRETGLELLRQTRDA
                     CLRERFSTTTLAFIDSLFAKERARNGDVDGAIESARSILERMSDTGEMLARGGVAAAL
                     VELLLTRGNDADLREARTLLDSLSADDSGYALDEMVSLILRAFLAQAEGDQPAYRDFA
                     ERFRTMATSLGAEGFMALAATMT"
     gene            complement(5818..6297)
                     /locus_tag="VFC01_19175"
     CDS             complement(5818..6297)
                     /locus_tag="VFC01_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81783.1"
                     /translation="MLAEISASVRQWLTNDTDLDEVIAEVRRLNPFFMVFAPKIQPGF
                     TTRLKSEQANIRKLLIRMDTMRRTSYVSAGYLIAEITALMILVLLMITEIGPLAPTIL
                     VVGVITGLMTYMLALIRDLDNPFEYRRGRPGAADVDLAVLEESEARLRSLAATIHSA"
     gene            complement(6258..6680)
                     /locus_tag="VFC01_19180"
     CDS             complement(6258..6680)
                     /locus_tag="VFC01_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81784.1"
                     /translation="MRYARKIRLRSIYCYRIPEAPRSKCEEALPRQPAAAIAGRAFCH
                     RHGRTQGLYDESELKHLDFSPLLTGVIAAEVFIIGFILSGTSADFKEAERLPGELAAS
                     LETIADECLIMDAELDLPEARKCLSVCWQRSARPSASG"
     gene            complement(6750..7592)
                     /locus_tag="VFC01_19185"
     CDS             complement(6750..7592)
                     /locus_tag="VFC01_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HZN81785.1"
                     /translation="MTAPAATRQRAPVVLLRPVPGDTVIHRLWAGTKLIAVAGIGVLL
                     TFYPGWVPIGLVAALILVTARLAHIQRGALPSIPRWLWVLLFLGGATATFAGGSPVID
                     LGSVELALGGLLSFLRITAVSIVLLGLGAMVSWTTNVAEIAPAVATLGRPLRALRVPV
                     DDWAVALALALRAFPMLIDEFRVLYAARRLRPKMVPTTRRARRRRWALEVVDLLAAGI
                     TVALRRADEMGDAITARGGAGQISAAPSRPKRADWVALSIVVVMCAVALALELTILGT
                     TYTR"
     gene            complement(7589..>8818)
                     /locus_tag="VFC01_19190"
     CDS             complement(7589..>8818)
                     /locus_tag="VFC01_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN81786.1"
                     /translation="KTTLMLMLAGRQPTSGTIERPGAVGLGKLGGAAVIMQHPESQVL
                     GTRVADDVVWGLPPGQTTDVHRLLTEVGLDGLAERDTGGLSGGELQRLAVAAALAREP
                     SLLIADEVTSMVDQDGRDGLMSVLSGLTQHHRMSLVHITHYNSEADAADRVINLTGNG
                     GAADNTEMVETAAAPAATVHAAHHSDEPVLEVNGVGHEYASGTPWATTALRDINFVVH
                     EGDGVLIHGLNGSGKSTLAWIMAGLTVPTVGNCLLDGAPVSEQVGAVAISFQAARLQL
                     MRSRVDLEVASAAGFSHRDHSRVVAALATVGLDSTLAKRRIDQLSGGQMRRVVLAGLL
                     ARSPRALILDEPLAGLDAASQRGLLRLLEDLRRRAGLTVVVISHDFSGLEDLCPRILH
                     LHDGALSPAQTAAGGVS"
BASE COUNT         1582 a         3038 c         2735 g         1463 t
ORIGIN      
        1 gccgttgtcg aacaccgaca cccgctccga cgagttcggc accgcgacga acggcgtgca
       61 ctccagcccc gtcgcggcag aatcggaata cagcgtcagc ggcagctcgg tgagcttctc
      121 ccccactcgg gtcccgccgc cgggcgccga caacgccgtg cggccctcct gcgcgcctcg
      181 gccatccatc gaccacgcca agtagatcat catgtcggcc actgtcgatg gcggcatgat
      241 cgtctcgtac cgccctgcgg acaactcgac ggtccgctgc gcccagccca gccgcgtcga
      301 cagctgctcg agcagcgaat caatgggcac atcaacgaaa tcgggcgtgc tgatacccgc
      361 ccacgcgctg gcgccatcgc gtttggcgtt gatctccacc gaccctgtcg gctgcgtgta
      421 ccgccgccgc agtccggtcg acgtggccac gaacgtcgtc tccacgatgt gccgggcgta
      481 tccgtacagc tgatccacgc cctgaaaacc gtgcgccaga ggggctgcga gtccagagaa
      541 cactcgcgcg ccggtactcg gcaccggggc atcccagtcg gccggcacct cggtgcccgg
      601 tagcggaggc gcaccgtcgc gcgctgcggg cgcggacacc gcagtctgct gcgactccgc
      661 gaccatggct gcgatcaccg cgggatccac gtcgctggat ctcactgagc ctacatgggc
      721 tgtatcgccc tgccgcacaa tcgatatcac ggtggtggtg cggctcaccg attcgccgtt
      781 ggtggtcatc gaattacccg cccagcgcag ggcggcgtcg gcgcgatcgg tgaccagcac
      841 gatcgtctcg tcggccttac ccaaccgcgc ggcctcggcc agcgctgtgt cgacgacttg
      901 ctgtgcggcg atcatcgctt accgtcccgc ctcggtgcgg gtgttgagga cgttgatgcc
      961 gcggaacaac gccgacgggc agccatggct gaccgccgcc acctggcccg gctgcgcttt
     1021 gccacagttg aatgccccgc cgagccgcca agtcgacggt ccgccaacca cttccagtgc
     1081 accccaaaag tccgtggtgg tcgcctgata ggcgacgtcg cgcaactgac cgtccagtcg
     1141 gccgtcgcgg atccggaaga accgctgtcc ggtgaactgg aagttgtatc gctgcatgtc
     1201 gattgaccat gatttgtcgc cgacgatata gatgccgtcc tcgacgcggg cgatcagatc
     1261 ttgggttgtc acatcatccg cagacggctg cagcgataca ttggccatcc gctggatcgg
     1321 cacatggtgc ggcgaatcag catacgagca gccgttggac cgcgatacgc ccagccgcgg
     1381 ggcgaacacc cgatccagtt ggtagccgac gaaaacaccc tcgcgcacca gatcccagcg
     1441 ctgcgcctgc acaccctcat cgtcgaaacc cacagtggcc aagccgtatt cgacggtccg
     1501 gtcggcggtc acgttcatca ccgaagagcc atagcgcatc gtgccgagtt tgtctggcgt
     1561 ggcgaacgac gtgcccgcat aggcggcctc gtagcctatc gcccggtcgt attccgtggc
     1621 gtggccgatc gactcgtgga tcgtcaacca caggttcgac gggtcgatca ccaagtccgt
     1681 tggaccggcc gataccgaag gcgctttggc cttctcggcg agcagcgacg gcagctgcgc
     1741 cagctcgtcg gtccagttcc acacgtcgtc gccggctgcg tactcccacc cccgcgccgt
     1801 cggcggcgcc agcgttcgca tggtctcgaa cgcggccgcc acggcgtcga ccgtgaccgc
     1861 ctcgaacgtc ggctgcaccc gcacccgctg ctgcgtgatc gacgagccga acgtgtcggc
     1921 gtagaacgtc tgctccttcg ccgcgtgcag cccagcggac acgtggtcga caccatcggc
     1981 agccagcagc cgtcccgagt actcgcccag caccgcgatc ttgtcggcga cgccgaccgt
     2041 gaacggatcg accaggtagt ccgataccca gctgacgtcg gagtacaccg gttcggcggc
     2101 cagctcgatg cgttcggcgt tcagcggcgc cagcgtcgtc gccacctcta cagcccgtcg
     2161 cgcggtctta gccgccacct cggggtcgag ctcggcatgc gaggcgaagc cccaagtgcc
     2221 gtccacgatg acccgcaccg ccaggccgat ctcgcggttg acgaccgacg tctccagttc
     2281 gccgtcgcgc agctgcacaa tctccgtcgt gatgcagtga atgcgcaggt cggcatagct
     2341 tgccccggcg gcagtcgccg tggagagcgc ggcgtcggcg agctgatggc ggggcaacgc
     2401 caggaagtcg gcatcgagcc tgggcgacgt tgtcacgtca ctaccgtagt ggccgcgtgt
     2461 ttcaatgatt cggcggcaac gccccgtgtc cggtcgacgg gcaccgggct cgcccacgcc
     2521 gcattagctc tgcggtgagg gcgcaaacgt gcgagcagag aacgcccagt gcgcagcgcc
     2581 aacgagcgtg gtggttagtc acgtcatcgt cgcggccaag gccataaacc cctcggcgcc
     2641 aagggatgtc gccatggttc ggaaacgctc ggcgaaatca cggtaggcag gctggtctcc
     2701 ttccgcctgg gccaagaacg cacgaaggat gagcgacacc atttcatcga gcgcgtatcc
     2761 ggaatcgtcg gcggacaggg aatcgagaag ggttcgtgcc tcccgcagat cggcgtcgtt
     2821 accccgagtc agcagtagct cgacaagagc ggccgccacg cctcccctgg cgagcatctc
     2881 gccagtgtcg gacattcgct cgaggatcga tcgcgccgat tcgatggctc cgtccacgtc
     2941 gccattgcgt gcccgctcct ttgcaaacag gctgtcgatg aatgccaggg tggtggtgct
     3001 gaatcgctcg cgaaggcaag cgtcacgggt ctgcctgagc agttccaggc cggtctctcg
     3061 gtcctcacct tcgcgataga gcaatgtgat gccacgcgtg aaacgtgccg cggccaacgc
     3121 gaaatcgtca ctggagcgtt cagccagctg cagtgcatcg gccgtgtcat ggacggctgc
     3181 ctcatccggc aggaatgctc ctgttgtgat gcccaggtat ttgtacatga ccgcgaagac
     3241 cagggaggac gggtcgtagg cacgtgccat cgcaacggcg cggtccgcgt cgtcgcgtcc
     3301 aggcgaaagc ccgatccggt agcgcaatgt cccccgaatc gctagcaaca atgccaacgg
     3361 tgagccaaag accaggttgc ctttggtcgg atcgccccca gccagatcga tagcgcgctc
     3421 ggcgatgcgt aacgcctcgt tcatttcgcc ggcctcgtac ttcgcgagca ccaacggaaa
     3481 cgccaacccg accgtcagcg cagggtcgcc gatcgactcg aacagctcca tacattctga
     3541 cgccagccgc gctgactccc gatgccgtga atgaaccgtc agggccatca cgaaacccga
     3601 catccccatc gccaacgacc gcttgtcccc ggacgcgtcg gtcaggtctt gcaattcagc
     3661 gaatcctgtg tcggcgacac ttccgcccac ccgaaatgcc gtagcgcaca gtaacgttcg
     3721 cggagcgata cgcattgcgg tgacattaag atcaccgtcc ggcatctgat cggcgacggc
     3781 ttgtgcacgc cgccaactga tccgcgcacc agccacatcg cgggtcgcca accaacccgc
     3841 tgcgcgcatg tgccacgcat acgcgccacc gagatcgact gccgcctcca agtgctcggc
     3901 gatcagcgct gcgctggcat cgactgcgtg cgcttcgcgc ttctcgatcg caattgccag
     3961 ccgccggtgc aagtccgcac gatccgcctt caactgagat tcgtacgcca cagtgcggaa
     4021 aagtgggtgc cggaatgcgt actcgcgctc cgcacctgaa gtaacctcat cgatgagctc
     4081 cgcttcggtc aatgccttga gcgccggatc gacgccgagg ctcaccagca agtccgccgt
     4141 gaagcgcgaa cccaccaccg ccgcggtccg caccgtccgc ttggccgccg ggcccaggcg
     4201 gtcgatgcgg gcggcaatcg tcgcctgcag cgttgccgga acgctgtcgt cggcgacatc
     4261 gccgcggcac acataattgc cgtgactgcc gaccagaacg tcccgttcgg ccaagtcacg
     4321 cacgatctct tcgacaaaaa acgggttccc ggcggcccgc tcggtcaccc tcgctccgat
     4381 ctgcgtgacg gaaggatcgg agcccagcag ctctgtcaac aacgtgatgg attgtgaatc
     4441 atccagtggg gcaaggctta tcctctggga ttcggccacc tccgtgagcg caccccggta
     4501 ttcgggtcgg taggtgaaga tcatcattga gtgcgactgc ttgatcgccg tgaggaagtc
     4561 ggccagcatc gattcactga cctcatcgat ccaatgtgcg tcttcgatca cgtagacagt
     4621 cggattcttg cgttccaaat gcgcggcagt gatgagcgca ttgagtcggc gacgacgggc
     4681 gtccgggtcg atgtcaggca gggccgcagt gggatcggcg atgcccagca gatcatcaag
     4741 cagcataagg tcagtggtgt ccgcgtcgac gaagcgcgat ctgacttgtt gtcgggcagc
     4801 tgcatcagaa aggccggcca ctgcgtaatc ggccttcagc agattggcca ccgcgtgaaa
     4861 cgcaatgtcg cgggcatggg cttcgcagta cgtgacatgc acgtccgccc cgcggtttcg
     4921 cgccagcgcc gccgcttcgc tgactaacct gctcttcccg atgcccgggg caccgacgac
     4981 acccacgact tgcccacgcc cagccaacac ctgctccatg gcctgcgcga tcctgtgcgt
     5041 ctccggctca cggcccacga gagtcgactg ccaccggcga ggttcgcccc gtcgcgcaat
     5101 gatccccacc aactggcggg cgggcaccgg gtctgctctg cccttgatct ggaccagttg
     5161 cggctcaccc agcgccgcag cgctttccac caaccgcgcg gtcgatgtgc tgagaatgac
     5221 cccgccgggc ggcgcgacgg actccatcct ctgcgccatc ccgacctggt caccgatcgc
     5281 ggtgtaaccc gcagcggtcg aaccgatttc gccggcgatt acctggccgg agttcaggcc
     5341 gatccgcagc tgaagcgtga tgtcgtcgcg ttcgtgcact tcggccgcct gacgctgcac
     5401 atcccgttga atgtccaggg ccgctcggca ggcgcgcaac gcatggtctt ccatcgccac
     5461 cggcgcaccg aacagcgcca tgattccgtc gcctgtgaaa ctctgaacag ttccgccgta
     5521 gcgctccaca acggcggtcg cgtgatcgac gaggccggcc atgatctctc tcaaccgctc
     5581 cggcccgacg gcgacggcga tgttcatcga ccgcacgacg tcggcgaaca acacggtcac
     5641 ctgcttgtac tcagcgggct cccgtgacgc cgtgatcgga gcgccgcagg catcgcagaa
     5701 ccgagcgcca cctcgcagtt ctgtgccgca cgactcgcac acggttggtg ccgacgtcat
     5761 cccgaaacct ccccccaagc cggccaccag gcgacgacgt gtgatgcctc gaaacgttca
     5821 tgcactatgg attgtcgcag ccagagatcg gagccgtgct tcactttcct cgagcactgc
     5881 cagatccacg tcggccgcgc ctggccgccc gcgacggtac tcgaacgggt tgtcgaggtc
     5941 gcggatcagc gccagcatgt aggtcatcaa gcccgtgatg acgccgacga ccaagatcgt
     6001 gggcgcgagc gggccgatct ctgtgatcat cagcaggacc aagatcatca gcgctgtgat
     6061 ctctgcgatg agatagccgg ccgagacata cgaagttcgc cgcatcgtat ccatccggat
     6121 caagagcttg cggatgttgg cttgctcgga tttcaaccga gtcgtgaagc ccggctggat
     6181 ctttggggcg aacaccatga agaacgggtt gaggcggcgc acctctgcga tgacttcgtc
     6241 gaggtcggtg tcattcgtca accactggcg gacggacgcg ctgatctctg ccagcacacc
     6301 gagaggcact tgcgtgcttc gggcagatca agctcggcat ccatgatcag gcattcgtcg
     6361 gcgatcgtct ccaggctcgc cgccagctcg ccagggaggc gctcggcctc cttgaaatct
     6421 gccgatgtgc cgctcaggat gaagccgatg atgaacacct cggcggcgat gacgccggtg
     6481 agcagcgggc tgaagtcgag gtgcttgagc tcgctctcgt cgtagagccc ttgagtacga
     6541 ccatgccgat ggcaaaaggc acggccagca atagcagccg cgggttgccg cggaagagcc
     6601 tcctcacact tagagcgtgg cgcttcgggg attcgatagc agtaaattga ccgaagtcgt
     6661 attttccgcg catatcgcat tgacgtgata tgcgttgact ctgcggtcag ggcgcaaaag
     6721 tgcgagggga caacgccgtt accgcagagt caacgcgtgt aggtcgtccc caggatcgtc
     6781 agctccagcg cgagcgcgac agcacacatg actacgacga tcgaaagcgc aacccagtct
     6841 gcccgtttcg gccgcgacgg cgccgccgag atttgacctg ctccgccgcg ggcggtgatc
     6901 gcatcgccca tttcatcggc ccgacgcagc gccacggtga tccccgcggc gagcagatcg
     6961 acaacctcca gtgcccaccg tcgcctgcgc gcccgtcgtg tcgtcggcac catcttgggt
     7021 cgcaaccggc gcgccgcgta gagcactcgg aattcgtcga tcagcatcgg aaatgcccgc
     7081 aacgccaacg ccaatgcgac cgcccagtcg tcgaccggta cccgcagcgc ccgtagtggc
     7141 cggccgagtg tagccaccgc gggcgcgatt tcggcgacgt tcgtcgtcca cgacaccatc
     7201 gcaccgagtc caagcagcac gatcgacacc gcagtgatgc gcaagaagct gagcagccca
     7261 cccaatgcga gttccaccga tcccaagtcg atcacggggc tcccaccggc gaacgtcgcg
     7321 gtggcaccgc cgaggaacag cagtacccag agccagcgtg gaatcgacgg caatgcgcca
     7381 cgctgaatgt gcgcgagcct tgccgtcacc aggatcaggg cggccaccaa gccgatcggc
     7441 acccaccccg ggtagaacgt caacagcacc ccgatgcccg ccaccgcgat gagttttgtt
     7501 ccggcccaca gccggtggat gacggtgtcg ccgggcaccg gccgcaacag caccaccggc
     7561 gcgcgctgcc gggtcgcggc gggggccgtc atgacacccc tcctgccgct gtctgcgccg
     7621 gggacaacgc gccgtcgtgc aggtgcagaa tgcgcgggca caggtcctcc aggcccgaaa
     7681 agtcgtggga gataacgaca acggtgaggc cggcgcgtcg ccgaagatct tccagcagcc
     7741 ggagcaggcc gcgttgactc gcggcgtcca ggccggccag cggctcatcg aggataagcg
     7801 cgcgcggtga gcgagccagc agaccggcga gcacgacgcg gcgcatctgg ccgccgctga
     7861 gctgatcgat gcgtcgcttc gccaacgtag aatcgagtcc gacggtagcc agcgccgcga
     7921 ccacgcggct gtggtcacgg tgcgaaaacc cagccgccga agccacttcc agatcgacgc
     7981 ggctacgcat cagctgcagc ctggccgcct ggaacgagat cgccaccgcg ccaacctgtt
     8041 cggaaaccgg agcaccgtcg agcagacaat ttccgacagt gggcaccgtc agacccgcca
     8101 taatccaggc cagcgtcgac ttgcccgacc cgttcaggcc gtggatcagc acaccgtcgc
     8161 cctcatggac cacgaagttg atgtcgcgca gagccgttgt ggcccacggc gttccactgg
     8221 cgtactcgtg tccgacgcca ttgacctcga gcaccggctc atcggagtgg tgcgccgcgt
     8281 gcaccgtggc ggcaggtgca gcggccgtct ccaccatctc ggtgttgtcg gcggcgccgc
     8341 cgtttccggt caagttgatc acgcgatccg cggcgtcggc ttcgctgttg tagtgggtga
     8401 tatggaccag cgacatgcgg tggtgctggg tcagcccgga cagcaccgac atcagcccgt
     8461 cgcgcccgtc ctggtcgacc atgctggtca cttcgtcggc gatcaacagc gacggctcgc
     8521 gcgccagcgc cgccgccacg gcgaggcgct gcaactcgcc gccggacagg ccgccggtgt
     8581 cacgctcggc cagaccgtcg aggccgacct cggtgagcag ccgatgcacg tcggtggtct
     8641 ggccgggcgg caggccccac accacgtcgt cggcgacgcg cgtgcccagt acctgactct
     8701 ccgggtgttg cattatcacc gccgcgccac cgagcttgcc gaggccgaca gcgccgggac
     8761 gctcgatggt gccggacgtg ggttggcgcc cggcgagcat cagcatcagt gtggtctt
//
LOCUS       DASRJA010000437         4764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4201615,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000437 DASRJA010000000
VERSION     DASRJA010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4201615"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..1829
                     /locus_tag="VFC01_19195"
     CDS             294..1829
                     /locus_tag="VFC01_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN81787.1"
                     /translation="MTRAAVLAAVGVAVAAVAALTACSTGDRVDLGGDSSGNLVAAIA
                     GEPDQLDPQKTSAYFSFEVLENVFDTLVEPDANLEMRPALAESWTVSPDQLVWTFHLR
                     RSVTFHDGSPFTADDVVYSYRRIIDQELANADKLSAVTGITAADPSTVVITLKQPTPN
                     LLTNLGGFKGLAIVQRRNVESGEIATHPVGTGPFAFQSQKSGDSIILKANPTYWAGAP
                     KVSGVTFRFISEPSTALSALQAGEIDWTDSVPTQRVAQLKDDDSITLATTASNDYWYL
                     ALNEARPPWNDLRVRQAIAYAIDRDAIVQATSYGTAVANQLAIPQGNPWYTPYDAYRH
                     DVDKAKSLLADAGAAPKSLDMLVTNEYPETVTAAQIIADNLAPLGITVNIRTVDFATW
                     LDEQNNGHFDMLMMGWLGNIDPDDFYYAQHHTNGSSNAQKFSNPEVDRLLDAGRVEIN
                     RNARAGEYAKAATIIADQVSYIYLYNPSVIQAWTTRLSGYEARRDGAIRFRTATLASG
                     GAA"
     gene            1826..2794
                     /locus_tag="VFC01_19200"
     CDS             1826..2794
                     /locus_tag="VFC01_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN81788.1"
                     /translation="MTAVFTHPITRFIARRLLYSLVVLFGVLVVVFALVHLVPGDPVR
                     IALGTRYTPEAYNALRSASGLDRPLVEQFFGYIGSALTGDLGVSFRNGDPVTVTLLER
                     LPATVSLGLVGILIALLIALPAGIWSALHEGRVSDAIVRITSQFGVSIPDFWLGILLI
                     ALFASTLGWLPTSGYRPLLDDPAGWLRHIVLPGLTVGLVAAAIMTRYVRSAVLEVASM
                     GYVRTARSKGLPPQVVTFRHTVRNALVPVLTITGIQLATILGGVIVVEVVFAWPGLGR
                     LVYNAVAARDYPVIQGAVLLIAALFLLINLIVDVLYAVVDPRIRLA"
     gene            2791..3672
                     /locus_tag="VFC01_19205"
     CDS             2791..3672
                     /locus_tag="VFC01_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN81789.1"
                     /translation="MTIATVETTRVASWRLLLRNPMTVVSAAVLGVVIVVAVAANWIA
                     PFGVNDVDVPNALQPPSGEHWFGTDELGRDVLSRVLVATQASMRVAVVSVAFAVVVGV
                     TVGVIAGYRGGWVDTVFMRVVDVMFAFPVLLLALAVVAILGPGTTTTILAIGIVYTPI
                     FARVARASTLSVRVEPFVQMSRAMGTGNLYILARHIIPNIAGPLIVQTSLSLAFAILS
                     EAALSFLGLGIQPPQPSLGRMIFDSQGFVTMAWWMAVFPGAAIVVIVLAFNLVGDGLR
                     DVLDPKQRTMIEARSKQ"
     gene            3669..>4764
                     /locus_tag="VFC01_19210"
     CDS             3669..>4764
                     /locus_tag="VFC01_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN81790.1"
                     /translation="MTDPVLSVRDLRVRIGRREIVHGVAFDVQREQTLGIVGESGSGK
                     TMTALAATGLLDTPGAVVSGSSRLAGETELVGGSARLLRSVHGGKIGFVFQDPGTSLN
                     PLLTLERQITESLETHRKMTRGQATTRALELLEAVGLPDPQTRLHAYPHQLSGGQRQR
                     VMVAIALACDPELLIADEPTTSLDVTTQAQIIDLVRELQQDFGTAVVWISHDLGVIGQ
                     VADDVTVLRDGQIVEQGPILDIFDEPQNDYTRELLHARPLVGRAEPAPAPNDAPVLLR
                     VDGLDVRFAVTTPVGRSTVHAVKRLSFEVQRGTTLGLVGESGSGKSTVAGALTGLVEP
                     DTGTAQLDGVEIFGVRGAAAKELRKKIGLVF"
BASE COUNT          790 a         1556 c         1566 g          852 t
ORIGIN      
        1 caaatcgccg accaccggca cacacgacgg ccagatttgg taggtgccgc tatcgccctg
       61 ctccggcgtg taatcgtaga tgccctgaac aagctggtca gcctgggccg gaccggcgaa
      121 ccccagcgtg gcgcaggcga ggacggcggc tgcggcgagc ccgcggatga ttctcacgcc
      181 gctgagtatc gcagtgttcg tttgacaaag tcacgggttc acggcggcgt ggggtttcga
      241 atgccccgcc catggggtaa agggccgagg gcgacggttc cggggtgatg gcgatgacga
      301 gagcagcggt gttggccgct gtcggcgtcg ccgtggccgc tgtcgctgcc ctcaccgcct
      361 gctcgacagg cgatcgggtc gatctcggcg gcgactcatc cggcaacctc gtcgcggcga
      421 tcgcaggcga gcccgaccaa ctcgaccccc agaagaccag cgcgtacttt tccttcgagg
      481 tcctggagaa cgtcttcgac acgctcgtcg aacccgacgc gaacctcgag atgcgtcccg
      541 cgctggccga atcctggacg gtgagccccg accaactggt gtggacattc catctgcgcc
      601 gcagtgtcac attccacgac ggcagcccgt tcactgccga cgacgtcgtg tactcgtatc
      661 ggcgcatcat cgaccaggag ctggccaacg ccgacaaact cagcgccgta acgggtatca
      721 cggcggccga tccgtcgacg gtcgtcatca cgctgaaaca gccaacaccg aacctgctca
      781 ccaacctcgg cgggttcaag ggactggcaa tcgttcagcg acgcaatgtc gagagtggcg
      841 agatcgctac gcatcccgtc ggcacggggc catttgcatt ccagagccag aagagcgggg
      901 actccatcat cttgaaggcc aacccgacgt attgggctgg ggcgcccaag gtttcgggtg
      961 tgacgttccg gttcatctcc gaaccgtcga cggcgctgtc ggcgctgcag gcaggcgaga
     1021 tcgactggac ggattccgtt cccacccaac gcgtcgcgca actcaaggac gacgactcca
     1081 tcaccctcgc gaccaccgcg agcaacgact actggtacct cgcgctcaac gaggcacggc
     1141 caccgtggaa cgacctccgc gttcgccaag cgatcgctta cgcgatcgac cgggatgcga
     1201 tcgtgcaggc gacaagctac ggcaccgcgg tggccaatca gcttgcgatc ccccagggca
     1261 acccgtggta caccccgtac gacgcctacc gccatgacgt cgacaaggcg aaaagcctgt
     1321 tggccgacgc cggagcagca cccaagagcc tcgacatgct ggtcaccaac gaatacccgg
     1381 aaacagtgac cgcggcgcag atcatcgcgg ataaccttgc gccgcttggc atcaccgtga
     1441 acatccgcac tgtagacttc gccacctggc tcgacgaaca gaacaacggc cacttcgaca
     1501 tgctgatgat gggctggctc ggcaacatcg accccgacga cttttactac gcccagcacc
     1561 acaccaacgg cagcagcaac gcccagaaat tctccaaccc ggaggtcgac cggttgctcg
     1621 acgcagggcg agtcgaaatc aaccggaatg cgcgtgccgg cgaatacgcc aaggcggcga
     1681 cgatcatcgc cgatcaggtc agctacatct acctgtacaa cccgtcggtg atccaggcct
     1741 ggacgacgag actgtcgggc tacgaggcac gccgcgacgg ggcgatccgg ttccgcaccg
     1801 cgacgctggc atcgggtggt gcggcgtgac ggccgtattc acccacccga tcacgcgctt
     1861 catcgcgcgt cggctgctct attccctggt cgtgcttttc ggcgtgcttg tcgtggtgtt
     1921 cgcgctcgtt catctggtcc ccggcgaccc ggtccgcatc gcgctcggca cgcgctacac
     1981 ccccgaggcc tacaacgcgc tgcgatcggc gagcggtctg gaccggccgc tcgtcgagca
     2041 gttcttcggc tacatcggct cggcgctcac cggcgacctg ggtgtcagct tccgcaacgg
     2101 cgaccccgtc acggtcacgc tgctcgagcg cctgcctgcg accgtgtcac ttggcttggt
     2161 gggcatcctg attgctctgt tgatcgcact gccggccggt atctggtcgg cgttgcacga
     2221 gggccgcgtc tcggatgcga ttgtgcgtat cacgagccag ttcggcgtgt ccattcccga
     2281 tttctggttg gggatcctgc tgatcgcgct gttcgccagc acgctcggct ggctgccgac
     2341 gtcgggctac cgtccgctgc tcgacgaccc ggcgggctgg ctgcgccaca tcgtattgcc
     2401 cggcctgaca gtgggtttgg tggctgcggc catcatgaca cgatacgtcc ggtccgcggt
     2461 cctggaggtt gcgtcgatgg gctatgtacg caccgcccgg tcgaaaggct taccgccgca
     2521 agtggtgaca ttccgccaca ccgtccgcaa cgcgctcgtg ccggtgttga cgatcaccgg
     2581 aatccagctg gcgacgatcc tcggcggggt gatcgtcgtg gaggtcgtgt tcgcctggcc
     2641 gggcctcggc aggctggtat acaacgcggt ggccgcccgc gactaccccg tcattcaggg
     2701 cgccgtgctg ttgatcgccg ccctgttcct gttgatcaac ctcatcgtgg atgtgctgta
     2761 cgcggtggtc gacccgagga tcaggctggc atgacaatcg ccaccgtcga aaccacaagg
     2821 gtcgcctcgt ggcggctgtt gctgcgcaat cccatgaccg ttgtcagcgc ggcagtgttg
     2881 ggtgttgtca tcgtcgttgc ggtggcggcc aattggatcg caccgttcgg tgtcaacgat
     2941 gtcgacgtgc cgaatgcgtt gcagccaccg agcggtgaac actggttcgg caccgacgaa
     3001 ctcggccgcg acgtgctgtc gcgcgtcttg gtggcgaccc aggcgtcgat gcgggttgcc
     3061 gtcgtgagcg tggcattcgc cgtcgtcgtc ggcgtgacgg tcggcgtgat cgccggctac
     3121 cgcggcggct gggtcgacac cgtcttcatg cgagtcgtcg acgtgatgtt cgcgttcccc
     3181 gtgctgttgc tcgccctggc tgtcgtcgcg atcctcgggc ccggcacgac cacaacgatt
     3241 ctggcgatcg gcatcgtcta cacgccgatc ttcgcgcggg tggcccgcgc gagcacgctc
     3301 agcgtgcgcg tcgaaccgtt cgtccagatg tcacgcgcca tgggcacggg aaatctctat
     3361 atccttgccc gccacatcat tccgaacatc gctgggccgc tgatcgtgca gacatcgctg
     3421 tcgttggcgt tcgcgattct gtccgaggcg gcgctgtcgt tcctggggct tggcatccag
     3481 ccccctcaac cgtcgctggg ccggatgatt ttcgactcgc aggggttcgt gacgatggcg
     3541 tggtggatgg cggtgttccc cggtgcggcg atcgtcgtca tcgtgctggc cttcaacctg
     3601 gtcggcgacg ggctgcggga cgtgttggat cccaagcagc gcaccatgat cgaggccagg
     3661 agcaagcagt gaccgacccc gttctcagcg tgcgcgacct gcgggtgcgc atcggccgtc
     3721 gcgagatcgt gcacggtgtc gccttcgacg tgcaacgtga gcagaccctg ggcatcgtcg
     3781 gtgaatccgg gtcggggaag acgatgacgg cactggccgc gacaggcttg ctcgatacac
     3841 ccggcgcggt cgtaagcggc agcagcaggc tggccggcga gaccgagctc gtcggcgggt
     3901 cggctcggct gctgcgcagt gtgcacggcg gcaagatcgg tttcgtgttc caggacccgg
     3961 gcacgtcgct gaacccgttg ctcacgctgg agcgccagat cacggaatcg cttgagacgc
     4021 accggaagat gacacgaggt caggcgacca cgcgggcgct cgagctgctg gaggcggtcg
     4081 gactgccgga cccgcagacg cgcctgcacg cctatcctca ccagttgtct ggcgggcagc
     4141 ggcagagggt gatggtcgcg atcgcgctgg cctgcgatcc cgaactgctg atcgccgacg
     4201 agccgaccac gtcgctggat gtgacaaccc aggcgcagat catcgacctc gttcgtgagc
     4261 tgcaacagga tttcggcacc gcggtggtgt ggatcagcca cgacctcggc gtcatcgggc
     4321 aggtggccga tgacgtgaca gtgttgcgcg acgggcaaat cgtcgagcag ggaccgatcc
     4381 tcgacatctt cgacgagccg cagaacgact acacccgcga actgctgcac gcacggccgc
     4441 tcgtcgggcg ggccgagcct gcaccggcgc cgaacgacgc accggtgctg ctgcgcgtcg
     4501 acgggctcga cgtgcggttc gcggtgacca cgccggtcgg caggtcgaca gtgcatgcgg
     4561 tgaagcggct ttcgttcgag gttcagcgag gcacgacact gggtttggtc ggcgagtcgg
     4621 gttcgggcaa gtcgacggtc gccggggcgt tgaccggcct cgtcgaaccc gatacaggca
     4681 ccgcgcagtt ggacggcgtc gagatcttcg gcgtacgggg tgccgcagcc aaggagctgc
     4741 ggaagaagat cggcttggtg tttc
//
LOCUS       DASRJA010000438         4387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_432366,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000438 DASRJA010000000
VERSION     DASRJA010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432366"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFC01_19215"
     CDS             <1..209
                     /locus_tag="VFC01_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HZN81791.1"
                     /translation="TALVGRDQAYLNEPWVNARTIRVDSTDVGFLDFDISDNEIEALY
                     AKGYAAAKKFLSTWDWHAYLKRFR"
     gene            209..862
                     /locus_tag="VFC01_19220"
     CDS             209..862
                     /locus_tag="VFC01_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZN81792.1"
                     /translation="MAGDVAAILPLPARFADNAFARLAGEPALARVARTMLGAAVVAV
                     AESLADSVGETLAAHRLSDVDVVVAEGPGSRARCLAAGVRYLKDLPRHVLIHDIRRPL
                     TPESLRDRVITALRAGSPAAMPVLSVTDSVKVVDANGSVTRTLDRSTLRAVQYPRGFT
                     VDQLSLLLAGRSSEEFDEVDEALRAGTPITFVDGDSGAFAVELPRDAALVEAIIADG"
     gene            complement(846..1562)
                     /locus_tag="VFC01_19225"
     CDS             complement(846..1562)
                     /locus_tag="VFC01_19225"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HZN81793.1"
                     /translation="MSAPPGPYAVGVVLAAGLGTRVGADGNKAYLLLAGQSMVAWSLN
                     AVAQVPQIGRSVLVYRRGEFDLARDTVTAELPDATVELVEGGDSRHASEFNVLRYLAD
                     DIESGAVDVVLIHDAARPLAGSDMFVTALEVARKFGGALPALTIADVVQQGPTGVAST
                     SGTGRLVRVQTPQAFRARPLLDAYRAAAAEGFEGTDTSSCIETYADVEVRTFPGDEGN
                     LKVTYAHDVAVAERLLNRRR"
     gene            1581..2138
                     /locus_tag="VFC01_19230"
     CDS             1581..2138
                     /locus_tag="VFC01_19230"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZN81794.1"
                     /translation="MQRPLTILITGAGIAGPTLAYWLVRGGHRPVLVERTAELRVGGH
                     AVDIRGTALQVIERMGLDTAVRDARTQILTLSAVRPDGARTYDVALRPMREMRGDREI
                     EIMRDDLVRILFDAVSDDVEVVFGDSVRALAQDGARVAVEFDRDVHREFDLVAGTDGQ
                     HSAIRELTMGPERGLTHHLGAYLSI"
     gene            2175..2771
                     /locus_tag="VFC01_19235"
     CDS             2175..2771
                     /locus_tag="VFC01_19235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81795.1"
                     /translation="MLYNEPGRAAAMFTVRGNQRAKVVLLFRSPPLDIDFGDPAAQYD
                     VLRRRFAGMGWHTGRLIETMPDAYDFYFDEMSQVRLPRWSTGRVALLGDAAFGPSPMS
                     GQGTSLALIGGFLLAHHLGAQSDPAVAFDLWENEFRPNIELNQALAADGMSTLLPASQ
                     LGILTRNQMMRVLPLLARFGRGFGGRLERASRAVTLPR"
     gene            complement(2755..3177)
                     /locus_tag="VFC01_19240"
     CDS             complement(2755..3177)
                     /locus_tag="VFC01_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZN81796.1"
                     /translation="MEQLSDDVVAFLSEGTRTGLLGYLAADGRPLVAPVWFVVDDGEL
                     AFNTGKNTAKGRALARDPRVVMCVDDSQPPYAYVQVQGVASISEDPQDVLDIATRAGG
                     RYMGADRAEEYGRRNGVPGELVVRIKPARVLTAFNVAE"
     gene            complement(3332..3913)
                     /locus_tag="VFC01_19245"
     CDS             complement(3332..3913)
                     /locus_tag="VFC01_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZN81797.1"
                     /translation="MSEEFSLKLLSQFAVYRGEQPVELPPACQRLIALVALKRRPVHR
                     LWVCAMLWPHAQTRRAIASLRSTMWRLRPLGADPLLEVDPQYIVLAPGVSVDWHNAVD
                     QIGQLLDHDEPVDPQFVAELLPLLRAGELLDGWSEPWATAERQRYRAMRKAARDTLGR
                     GTEKQVANYACGSMRSLHTIHSVRKTKDDSREP"
     gene            complement(3984..>4387)
                     /locus_tag="VFC01_19250"
     CDS             complement(3984..>4387)
                     /locus_tag="VFC01_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HZN81798.1"
                     /translation="YVKGAATEVVGIEDAATALLLAAEKGRNGERYIISERYITIREL
                     FDAAADAGGAKRPRIGIPVWALHVLGYAGDVVGRLARRDTPVTSTSARLSHILPPMDH
                     GKAERELGWHPSPIHDSIRRAAQFYRENSVQ"
BASE COUNT          710 a         1473 c         1429 g          775 t
ORIGIN      
        1 cgacggcgct tgtcggtcgc gatcaggcct acctcaacga gccatgggtg aacgcccgca
       61 ccatccgggt cgactcgacc gacgtcggct tcctcgactt cgacatctcc gacaacgaga
      121 tcgaggccct gtacgcgaaa ggttatgccg ccgcaaagaa attcctgtcc acctgggatt
      181 ggcacgccta cctcaagcga ttccgctgat ggccggtgat gttgcggcga tcctgccgct
      241 gcccgcgagg ttcgcggaca acgcgttcgc ccgattggca ggggagccgg cattggcgcg
      301 cgtcgcgcgg accatgctcg gcgccgccgt agtcgctgtt gccgaatcgc tggccgattc
      361 cgtgggagaa acccttgcgg cccataggct ttcagacgtt gatgtcgtag tggccgaggg
      421 tccgggttcg cgggcgcgat gcctggccgc cggagtgcgg tacctgaaag acctaccgcg
      481 ccacgtcctg atccacgaca tccgtcgacc gttgacgccg gaaagcttgc gcgacagggt
      541 gattaccgca ctgcgggccg gcagccccgc cgcgatgccg gtgctgtcag tgaccgacag
      601 cgtgaaagtc gtcgacgcga acgggtcggt aacccgcact cttgaccgct cgacgctgcg
      661 cgccgtgcag tatccgcgcg ggttcacggt cgatcaactg tcgctgctgc tggccgggcg
      721 cagctccgaa gaattcgacg aggtcgacga ggcgcttcgc gcaggcacgc cgatcacgtt
      781 cgtcgacggt gattccggcg cgttcgcagt cgaattgccc agggacgccg cccttgtcga
      841 ggcgatcatc gccgacggtt gagcagacgc tccgccacag cgacatcgtg cgcataggtc
      901 accttcaggt tgccctcgtc gccaggaaag gtgcggacct ccacatcggc ataggtctcg
      961 atgcacgacg aggtgtcggt accttcgaac ccctctgccg cggcggcccg atacgcgtcc
     1021 agcagcggtc gcgcccgaaa cgcctgcggg gtttggacgc ggaccaaccg acccgtgccg
     1081 ctggtcgacg cgacacccgt cggtccctgc tgcaccacgt ccgcgatggt caacgccggt
     1141 agcgcgccgc cgaacttacg tgccacctcg agggcggtga cgaacatgtc gctgcctgcc
     1201 aacggtcggg cggcgtcgtg gatcaacacc acgtcgaccg cgcccgattc gatgtcgtcg
     1261 gcgaggtagc gcagcacgtt gaactctgag gcgtgcctgc tgtccccgcc ctcgacgagt
     1321 tcgaccgtcg cgtcaggcag ttctgccgtc acggtgtcgc gggcgaggtc gaattcgccg
     1381 cgccgataca ccaacaccga acggccgatc tggggaacct gcgcgaccgc gttcagcgac
     1441 caggccacca tgctctggcc cgccagcagc agatacgcct tgttgccgtc ggcgccgaca
     1501 cgcgtgccaa gccccgcggc cagcaccacc cctactgcgt agggccccgg cggtgcgctc
     1561 atggccgata ttttggcatt gtgcagcggc cgctcacgat cctgatcacc ggtgccggca
     1621 tcgcaggccc gacgttggcc tattggctcg tgcgcggagg tcatcggccg gtgctggtcg
     1681 agcgcaccgc cgagctccgc gtcggcgggc atgccgtcga catccgtggc accgcccttc
     1741 aggtcatcga acggatggga ctcgatacgg cggtgcgcga cgcccgcacg cagatcctca
     1801 ctctgtcggc ggtgcgaccc gacggtgctc gcacctacga cgtcgcgctg cggccgatgc
     1861 gtgagatgcg cggcgaccgc gaaatcgaga tcatgcgtga cgacctggtc cgcatcctct
     1921 tcgacgcggt gagcgacgac gtcgaggtgg tgttcggcga ctcagtgcgg gcgcttgcac
     1981 aggacggtgc acgcgtggcc gtcgaattcg atcgcgacgt acaccgtgag ttcgatctgg
     2041 tcgccggcac cgacggccaa cactcggcca ttcgcgaact cacgatgggc cccgagcgcg
     2101 gactcaccca ccatctcggt gcgtatctgt cgatctagac cgtcgacaat ctgctcgggc
     2161 tgcgtgacca agcagtgctc tacaacgaac ccggccgcgc cgccgcaatg ttcacggtgc
     2221 ggggcaacca gcgcgcaaag gtggttctgc tgtttcggag cccgccgctt gacatcgact
     2281 tcggcgatcc cgccgcccaa tacgatgtgc tgcgtcggcg cttcgccgga atgggttggc
     2341 acaccggtcg tttgatcgag acgatgcccg acgcgtacga cttctacttc gacgagatga
     2401 gccaggttcg gctgccgcga tggtcgaccg ggcgggtggc gctgctcggc gacgccgcgt
     2461 tcggtccgtc gccgatgtcg ggccagggca cctcactcgc gttgatcggc ggctttctgc
     2521 tcgcccacca tctgggcgcg cagtcggatc cggcggtggc gtttgacctg tgggagaacg
     2581 agtttcggcc caatatcgaa ctcaatcagg cccttgccgc cgacggaatg tcgacactgc
     2641 tgcccgcgtc acagctcggc atcctcacgc gtaaccagat gatgcgggtg ctgccgctgt
     2701 tggcccggtt cggacgcggc ttcggtggtc gcctcgaacg cgcctcgcgc gcagtcactc
     2761 tgccacgttg aacgccgtga gcaccctggc cggcttgatc cgcaccacga gctcaccggg
     2821 aacgccgttg cggcggccat attcctcggc gcggtcggca cccatgtagc ggccgccggc
     2881 cctggtcgcg atgtcgagaa cgtcctgcgg atcctcgctg atcgacgcga caccctgaac
     2941 ctgaacatac gcataaggcg gctgcgagtc gtcgacgcac atcaccacgc gcggatcgcg
     3001 tgccagcgcg cggcctttcg cggtgttctt tccggtgttg aatgccagct cgccgtcgtc
     3061 taccacgaac cagactggtg ccaccaaggg tcggccgtcg gcagccagat aacccagcag
     3121 accggtgcga gtgccttcgg ataagaatgc gacaacgtcg tcggaaagtt gttccacgcc
     3181 atcacggtag gccgccataa aggctggcgt cgatgggttc agtcctctgc gtggcggttg
     3241 tagtcccacc gctcgagacg cgtctgcagg tcgtacaggc ccagtgcggc aagcggtgtg
     3301 ccgacggaca tgcagatcag cagtagcgcg ttcatggttc tctcgagtcg tccttggtct
     3361 tcctgacgga atgaatcgta tggagcgacc gcatggagcc acacgcgtaa ttcgctacct
     3421 gcttttcggt accgcggccc aacgtgtcac gcgcggcctt gcgcatcgcg cggtaccgct
     3481 gacgctcggc ggttgcccac ggctccgacc atccgtcgag cagctctcct gcgcgcagta
     3541 gcggaaggag ttcggccacg aattgcgggt cgacgggctc gtcgtgatcg agcagctgcc
     3601 cgatctggtc cacggcgttg tgccagtcca ccgaaacgcc gggcgcgagc acgatgtact
     3661 gcggatcgac ctcgagtagt gggtcggcgc ccagcggccg cagccgccac atcgtggagc
     3721 gcagacttgc gatggcgcga cgcgtctggg catgcggcca cagcatcgcg cagacccaca
     3781 ggcggtgtac cggccgccgc ttcagcgcga cgagtgctat cagtcgttgg catgccggag
     3841 gcagctcgac gggctgttca ccccggtaga cggcgaactg actgagcagc ttcagcgaga
     3901 attcttccga cacatcttga tcgtcgagcc gtggttcgca gaaggcatgc gtagtcgact
     3961 acctgttttt ggccgcgcga cggctactgc acggagttct cccggtagaa ctgcgcggcc
     4021 cggcggatcg aatcgtggat cggactcggg tgccagccca gctcccgttc ggccttaccg
     4081 tgatccatcg gcggcaggat gtgcgataac cgggcacttg tactcgtgac gggtgtatcg
     4141 cggcgggcca gccggcctac gacatcaccg gcgtagccca gtacgtgtag cgcccacacg
     4201 gggattccga tccgcggtcg cttggctccg cccgcgtcgg cggcggcgtc aaagagttcg
     4261 cggatcgtga tgtagcgctc ggaaatgatg taacgctcgc cattgcgacc cttctcggcc
     4321 gcgaggagca gcgcggtcgc ggcgtcctcg ataccgacga cctcggtggc cgcgcccttc
     4381 acgtaga
//
LOCUS       DASRJA010000439         5721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2468551,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000439 DASRJA010000000
VERSION     DASRJA010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2468551"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFC01_19255"
     CDS             complement(<1..587)
                     /locus_tag="VFC01_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN81799.1"
                     /translation="MYDLKITGGTVVDGTGADRYPADIGVKDGRIVEVRRRNGDDAGL
                     EADAVETIDATGRIVAPGFVDIHTHYDGQVTWDPLLEPSSLHGVTTVVGGNCGVGFAP
                     VTPGREQWLIELMEGVEDIPGSALAEGITWQWESFPEYLDAIEKRPLAVDFGTQIAHG
                     AVRGYAMGDRGARNEEAGDDDIATMARIVQEAIEAG"
     gene            complement(597..1040)
                     /locus_tag="VFC01_19260"
     CDS             complement(597..1040)
                     /locus_tag="VFC01_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN81800.1"
                     /translation="MSVDDTVRDLWKALSARDFELAKTYLADDCIYFDVPVGPAAAAR
                     GPDDIEKRIRIAWDALSHYENHDGLLLSNGTDVMYEHSETWHWRSGETATLPFVTVHR
                     VENGQITLWKDYWDMGGLANFAPPGWLEDFANADMSWVFDATGLV"
     gene            complement(1030..2814)
                     /locus_tag="VFC01_19265"
     CDS             complement(1030..2814)
                     /locus_tag="VFC01_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN81801.1"
                     /translation="MPYDVIIRNGRWFDGSGAASAVRNVGVRDGHVVAISPDDLDDAD
                     CPQVIDATGKWVLPGMLDIHTHYDIEVLNGPSLSESLRHGVTTVMLGSCSLSTVHVDG
                     TDAGDIFGRVEAIPREHVISAIDNHKTWTNAEEYIQALEARPLGPNLAAFIGHSDMRA
                     ATMGLDRATQKRQRPTKREQAQMERMLSEALRAGFVGMSSQQLLFDKLDGEVCRSRTL
                     PSTYAKPRELRRLKSLLRRAGRILQSGPDIQNPFNLGSQLAQSLGFIRNPLKTSLLSA
                     ADVKANPYAIWAMGPAARLINCLGGNFRWQHLPVPFEVYADGIDLVVFEEFGAGAAAL
                     HLRDEVERNDLLRDESYRREFRKQYESKFGVRVWHRDFFDADIVDCPDASVIGKSFGE
                     VGRDRGGLHPVDAFLDLVLEHGRAVRWRTTISNHRPDVLKKLARDPGIQMGFSDAGAH
                     LRNMSFYNMGLRLLRHVNDAEKSGKPFLTVEQAVHRLTGELADWYRIDAGHLRIGDRA
                     DLVVIDPERLDATLDEYAEERVEQYGGLSRMVNRNDDTVNAVFVGGRAVFLDGTPTDL
                     VGTQRTGRFLRAAHRAPAVQESELASVS"
     gene            complement(2903..3826)
                     /locus_tag="VFC01_19270"
     CDS             complement(2903..3826)
                     /locus_tag="VFC01_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN81802.1"
                     /translation="MDWTPDEDGLRGRIALVAGATRGAGRGIAAALGEAGATVLCTGR
                     SSATGNMQSDYDRPETIEETAELVTSLGGTGIPIQVDHLDIAQVQALADRIRAEHGAI
                     DILVNDIWGAEVLKGHPPTWNRPIWEHDLDDGLRILRLAIDTHLITAHCLFPLLVARP
                     GGLHVEVTDGTKEYNDHTYRISVFYDLSKVAVNRLGYSLGHELERFGGAAVVVTPGWL
                     RSEMMLDNWGVTEDNWHTALDPNRSDGPTAPPGFAVSESPRYVGRGIAAMAADEDRSR
                     WNQRSVTSADLAREYGFTDIDGHQPDGWAEL"
     gene            3845..4756
                     /locus_tag="VFC01_19275"
     CDS             3845..4756
                     /locus_tag="VFC01_19275"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZN81803.1"
                     /translation="MTPLQRYIAEEIATDHVDGLLSRREALRRLALLGVSTAAATALI
                     AACGENKKTTSDAPVTSSGPATSSAAPPGSANALPTTPITWAGPQGELQAAWAQAPDA
                     RGGILVIHENKGLNDWVRSVAGRFAGIGYSGLAIDLLSGQGGTATFADPAAATAALGK
                     IPPEEFIANLKSGIDELGRRVPNKKLAVVGFCMGGGLTWQLLASGTPQLAAAFPFYGP
                     APENPDFAGSKDVAVLAFYGEQDQRVNATEPVAKAALEKAGLVHELVTEPNANHAFFN
                     DTGERYNATAADDAWRRVQDWLTQHVG"
     gene            4841..>5721
                     /locus_tag="VFC01_19280"
     CDS             4841..>5721
                     /locus_tag="VFC01_19280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN81804.1"
                     /translation="MRVVQVANFYGPRSGGLRTAVDRLGAEYCAAGHDVFLIVPGRHS
                     EWRELPSGVVRISLPARLIPFTGGYRAVLPGPVTSLLAELSPDALEVSDRLTLRSLGS
                     WGRRHDVGTVMISHERLDRLVGQILPARLARSIANVANRRTAASYDAVVCTTAFAREE
                     FDRIHAPNVVTVPLGVDLDQFHPRRHSATVRQRWARPEQTLLVHCGRLSVEKHAHRSI
                     DTVAALRDSGVDARLVVVGEGPMRARLERQAARLPVDFTGFIGCRDTVATILATADVA
                     LAPGPHETFGLAALEALA"
BASE COUNT          900 a         1923 c         1884 g         1014 t
ORIGIN      
        1 ccagcctcga tggcctcctg gacaatgcgc gccatcgtcg cgatgtcgtc gtctcctgcc
       61 tcctcgttgc gggcaccgcg gtcgcccatc gcgtagccac gcaccgcgcc gtgcgcgatc
      121 tgcgtgccga aatcgactgc cagggggcgt ttctcgatcg cgtccagata ctcggggaag
      181 ctttcccact gccaggtgat gccctctgcc agcgcgctgc cggggatgtc ctcgacgcct
      241 tccatcagct cgatgagcca ctgttcgcgg ccgggggtga ccggcgcgaa tcccaccccg
      301 cagttgccgc cgaccaccgt ggtgacaccg tgcaggctcg acggctccaa caacggatcc
      361 caggtgacct gtccgtcgta gtgggtgtgg atatcgacga aacccggtgc cacgatgcga
      421 cctgtggcat cgatggtctc gaccgcgtcg gcttcgaggc ctgcatcgtc gccgtttcgc
      481 cggcgcactt cgacgatccg accatccttg acgccgatgt cggcggggta tcggtcggca
      541 cccgttccgt cgacgactgt cccgccggtg atcttcaggt cgtacatcgg tccccctcag
      601 accaggccgg tggcgtcgaa tacccacgac atgtcggcgt tggcgaagtc ctccagccaa
      661 ccgggcggag cgaagttggc cagaccgccc atgtcccagt agtccttcca cagcgtgatc
      721 tggccgtttt cgactcgatg cacggtgaca aacggcagtg tggctgtttc tcctgagcgc
      781 caatgccatg tctcggaatg ctcgtacatc acatcggtgc cgttgctgag cagcaatccg
      841 tcgtgattct catagtggga caacgcgtcc catgcaattc tgatccgctt ttcgatgtcg
      901 tcgggtcccc gcgccgccgc cgcggggccg accggcacat cgaagtagat gcaatcgtca
      961 gcgagatacg tcttcgctag ctcgaagtcg cgggcggaca gcgctttcca caggtcgcga
     1021 accgtatcgt caactgacac tggccaactc actttcctga accgcggggg ctctgtgcgc
     1081 ggcacgcaga aagcgcccgg tgcgctgcgt gccgacgaga tcggtgggcg tgccgtccag
     1141 gaacaccgca cggccgccga cgaacacggc gttgaccgtg tcgtcgttgc ggttgaccat
     1201 gcgcgacagc ccgccgtact gctcgacacg ctcctcggcg tactcgtcca gcgtcgcgtc
     1261 gaggcgttcg gggtcgatga ccaccaggtc ggcccggtcg ccgatgcgca gatggccggc
     1321 gtcgatccga taccagtcgg ccagttcacc ggtgagccgg tgcacggcct gttcgacggt
     1381 caggaacggc ttgccggact tctcggcatc gttcacgtgc cgcagcagcc gcaggcccat
     1441 gttgtaaaac gacatgttcc gcaggtgcgc tcccgcatcc gagaagccca tctggatgcc
     1501 gggatcgcgc gcgagctttt tcagcacgtc gggccggtgg ttggaaatcg tggtgcgcca
     1561 ccgcaccgcc ctgccatgct cgagcacgag atccaggaag gcgtcgacgg gatgcaggcc
     1621 gccgcggtcc cggccgacct caccgaagga cttgccgatc accgaagcgt ctgggcagtc
     1681 gacgatgtcg gcgtcgaaga aatcgcggtg ccacacccgc accccgaact tgctctcgta
     1741 ctgtttgcgg aattcgcggc gatacgactc gtcgcgcaac aggtcattgc gctccacctc
     1801 gtcgcgcagg tgcagcgccg cggcgccagc gccgaactcc tcgaacacca caagatcgat
     1861 gccgtcggcg tacacctcga acggcaccgg cagatgctgc caacggaagt tgccgcccag
     1921 acagttgatc agccgggcgg ccggacccat ggcccagatg gcgtacgggt tggccttgac
     1981 gtcggccgcc gacagcaggc tggtcttgag agggttgcgg atgaagccca gcgactgggc
     2041 cagctgcgac ccgaggttga acgggttctg aatgtcgggg ccggactgca ggatccggcc
     2101 tgcgcggcgc aacagcgact tcagccggcg cagttcacgc ggcttggcgt acgtcgaggg
     2161 cagggtgcgc gagcgacaga cttcgccgtc gagcttgtcg aaaagcagtt gctgcgacga
     2221 cattccgacg aagccggccc gcagtgcctc cgagagcatc cgctccatct gggcctgttc
     2281 gcgcttggtg gggcgctgcc gcttctgcgt cgcccggtcc aggcccatgg tggcggcccg
     2341 catgtcggag tggccgatga acgccgccag gttggggccg agcggtcgcg cttccagcgc
     2401 ctggatgtac tcctcggcgt tcgtccaggt cttgtgattg tcgattgcgc tgatgacgtg
     2461 ttcgcggggg attgcctcga cccggccgaa gatgtcgccg gcgtcggtgc cgtcgacgtg
     2521 gacggtcgac agcgaacacg aacccagcat caccgtcgtc acgccgtggc gtaacgactc
     2581 cgacaacgac ggcccgttga gcacctcgat gtcgtagtgc gtgtggatgt cgagcatgcc
     2641 cggcaacacc cacttgccgg tcgcatcgat gacctgcggg caatccgcgt cgtcgaggtc
     2701 atcgggcgaa atcgccacga cgtggccatc gcggacgccg acgttgcgca ccgccgatgc
     2761 ggcgccgctg ccgtcgaacc aacggccatt gcggatgatc acgtcatagg gcaccaagtc
     2821 atagaacagc gcgtgctggc gggtgtcaac gaaatcggtg aacagatttc aagaaatgtc
     2881 tactgtagat gctgacctgg tgttacagct cggcccagcc gtctggctgg tggccatcga
     2941 tgtccgtgaa cccgtactcg cgggccagat cggccgacgt caccgagcgc tggttccagc
     3001 gcgaccggtc ctcatcggcg gccatcgcgg cgatgcccct gccgacgtat cgcggcgact
     3061 ccgagaccgc gaatcccggg ggagcggtgg ggccgtcaga ccggttcggg tcgagggcgg
     3121 tgtgccagtt gtcctccgtc acgccccagt tgtcgagcat catctccgag cgcagccagc
     3181 ccggcgtcac gacgacggcg gcgccgccga accgctcgag ttcgtggccg aggctgtacc
     3241 cgagcctgtt caccgcgacc ttcgacaagt cgtagaagac cgagatccgg taggtgtgat
     3301 cgttgtattc ctttgtcccg tcggtcactt cgacgtgcag gccgcctggc cgcgcgacga
     3361 gtagcgggaa caggcagtgt gcggtgatga ggtgagtgtc gatggcgagc ctcagtattc
     3421 ggaggccgtc gtcgaggtcg tgctcccaga tcggccggtt ccacgtcggc gggtgccctt
     3481 tgagcacctc ggcgccccag atgtcgttga ccaggatgtc gatggcgccg tgctcggccc
     3541 ggatgcggtc ggcgagtgcc tgcacttgag cgatgtcgag gtggtcgacc tggatcggaa
     3601 ttccggtgcc accgagtgag gtgaccagtt ctgctgtctc ttcgatggtt tccggacggt
     3661 cgtagtcgga ctgcatgttt ccggtggcgc tgctgcggcc ggtacagagc accgtcgcac
     3721 ctgcttcgcc gagcgcggcg gcgattccac gcccggcgcc gcgggtcgcg ccggccacga
     3781 gtgctatgcg gccgcgcagc ccatcttcat ccggcgtcca gtccataggt gcatactcgc
     3841 gaatgtgaca ccgctacagc gctatatcgc cgaagaaatt gccaccgatc atgtcgacgg
     3901 tttgttatcc agacgcgaag ccctgcgccg gctcgcgttg ctcggggtga gcaccgcggc
     3961 ggccactgcg ctgatcgccg cgtgcgggga gaacaagaag accacctcgg acgccccggt
     4021 gacctccagc ggccccgcta cgtcgagcgc ggctccgccg ggatcggcga acgcgctgcc
     4081 caccacgccg atcacctggg ccgggccgca gggtgagcta caggccgcgt gggcgcaggc
     4141 gccggatgcg cgcggcggca tactcgtcat ccacgagaac aaggggctca acgactgggt
     4201 tcgctcggtg gccggccggt tcgccgggat cggctactcg ggcctggcga tcgatctgct
     4261 ttcggggcag ggcggcacgg cgacgttcgc cgacccggcg gcggccaccg cggcgctcgg
     4321 caagattccg cctgaggagt tcatcgccaa tctcaagtcg ggcatcgacg agctgggccg
     4381 ccgcgttccg aacaagaagc tcgctgtcgt cggcttctgc atgggcggtg ggctgacgtg
     4441 gcagctgctc gcgtcgggta cgccccagct ggccgccgcg ttcccgttct atggccccgc
     4501 accggagaat ccggacttcg cgggctcgaa agatgttgct gtgcttgcgt tctacggtga
     4561 acaagaccag cgcgtcaatg ccaccgaacc cgtcgccaag gccgcgttgg aaaaagccgg
     4621 gctcgtgcac gaactcgtca ccgagcccaa cgccaaccac gcattcttca acgacaccgg
     4681 cgaacgctac aatgcgaccg ccgcggatga cgcgtggcgc cgggtgcagg actggctcac
     4741 ccaacacgtg ggttagttct cgcggtaatt cacgacctgt tttcctgccg gttgatgtcg
     4801 cgttgttgac cgcacacgcg tgcccgctct gattctcctc atgcgggtgg ttcaggtggc
     4861 gaacttctac ggcccgcgct cggggggcct tcggacagca gttgatcgcc tcggcgcgga
     4921 atactgcgcc gcaggacacg acgtgttcct catcgtgccg gggcgccatt ccgaatggcg
     4981 ggaacttccc tccggtgtcg tccgtatctc cttgcccgcc agactgattc cattcaccgg
     5041 cggatatcgc gcggtgttgc cgggcccggt cacgtcgttg ctcgcagagc tgtcgcccga
     5101 cgcactggag gtgtccgacc ggctgaccct gcgctcgctg ggcagctggg gccgccgaca
     5161 cgacgtgggc accgtgatga tctctcacga acgcctcgat cgccttgtcg ggcaaatact
     5221 tccggcccgc ctggcgcggt cgatcgccaa cgtggccaac cgccgaaccg ccgcgagcta
     5281 cgacgcggtt gtctgcacga cggcgtttgc tcgcgaagag ttcgaccgca tccacgcgcc
     5341 gaacgtcgtg acggtgccgc tgggcgtcga cctcgaccag ttccatcccc gccgccactc
     5401 ggcgacggtg cggcagcgct gggcgcgccc cgaacagacg ctgctcgtac actgcgggcg
     5461 gctctccgtc gaaaagcacg cccatcgcag catcgatacc gttgccgcgc tgcgcgattc
     5521 gggcgtcgac gccaggctgg tggtggtcgg cgaaggtccg atgcgcgccc ggctcgagcg
     5581 ccaggccgcc cgcctccccg tcgacttcac cggcttcatc gggtgccgcg acaccgtggc
     5641 gacgatcctg gcgactgcgg acgtcgcgct tgcccccgga ccgcacgaga cgttcgggtt
     5701 ggcggccttg gaggcgctgg c
//
LOCUS       DASRJA010000440         4398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1195267,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000440 DASRJA010000000
VERSION     DASRJA010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195267"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VFC01_19285"
     CDS             <1..1005
                     /locus_tag="VFC01_19285"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HZN81805.1"
                     /translation="IRFAMAEGRRGAFTPKSLVVGHDSFKGMSIEFGDLSGTGKFDMF
                     VSNITTPWGLEESNFVWHNTARSPAEARSKLSQGVAPFDNRAAAMNLAWVGWGWDAKM
                     ADFDNSGVMSIVQTCGFVKGNINRWNWLQELAMSNDVLLEEPNMWPKAEVGDDIAGSQ
                     PIAFWVREDNGRYVNLSTDLGFDSTTTPTRGVAVGDVNADGGQDFAVARQWGPPAFYR
                     NGNVGRDNYIGLRLYRPAVGGTAGADAPIGAPAYGAQARIITADGRTQVAQVDGGGGH
                     SGKRSFDIFFGLGKAGDRPASVELMWRDNDGMAHRQTFDLMAGWHNFVLTDQAQEVTS
                     "
     gene            1002..2048
                     /locus_tag="VFC01_19290"
     CDS             1002..2048
                     /locus_tag="VFC01_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014985433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enediyne biosynthesis protein"
                     /protein_id="HZN81806.1"
                     /translation="MTTDVRYAGGPDTRTVTTGPNGLRDQVKPPTVARAPAVPRVDPR
                     DPRYLALRNFAISISVFNILGYTVLGFEQPWTWPFFALAVGYSIEIAIELIAARAHRR
                     PPAFVGNGMWGLYTFLLPTHITALAANMLLYANTDFWPIAFAVAVAIGQKAVMVAPIK
                     GRMRHFMNPSNFGITVTLVCFSWVNIAPPYHFTEHVPDVIRIMVPIIIITAGTVLNAL
                     LTKKVPLIVGWVGAFVIQALIRWWIWDVHLWAALAPMTGVAFVLFTNYMITDPGTTPS
                     SGRSMFMFGSIVGTVYGVLMVFNVVYTLFFAVTIVCLGRGLLWWWKSLRERRSPYTPV
                     GRPGLLAPASPSAG"
     gene            2200..3516
                     /locus_tag="VFC01_19295"
     CDS             2200..3516
                     /locus_tag="VFC01_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZN81807.1"
                     /translation="MSTHQASALLLGLAAIILGAGLLGWVARKLGQPAVIGEVIAGIL
                     MGPTLFGGAIAGFLLPTDIRPFLSALANVGVAVFMFLVGLELNRSMLRGTGRVAATVS
                     LTSILLPCTLGILLGTYLLRTHPSKDRLGFVLFMGVAMSITAFPVLARILIDRELNRL
                     PLGGLALTCAAIDDVLAWSLLAVVVTVLGGDADQWRMLLAVPYLLLMLYPVRRLLGRL
                     VSARQRTGRLTTGQIAIVLAGLLVSGGVTEWIGLHFIFGAFLFGLVMPRGEKAELRTE
                     LHDRIGLVNNVLLLPVFFIVAGLRVDLSKVDAVDLAELGLILLVAIGGKFIGAFIAAR
                     LNGLGNRIAATLATLMNTRGLTELIILSVGLQLGVLDTGLYSLMVVMAVVTTAMAGPL
                     LSILYPRRLVARDIAATEAAVHAAEPGLHPLWIGGRGPISSASPRS"
     gene            3722..>4398
                     /locus_tag="VFC01_19300"
     CDS             3722..>4398
                     /locus_tag="VFC01_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81808.1"
                     /translation="MTIAIDLKPPADLLPRQPQPHALPPLLERSDLWTDFMVADARIP
                     MYDVPFVSIGGGMGSFMTVDYLRAAGGMPARDIRVLSNLTHPWQTYEKLATASQISRA
                     RRIRSDAASRPDNLWGFPSFALQEALKDRTIKPLIQVLLEPVFADFYTPKLGDVLGGM
                     EREANRIGYWDMLVRGDVQIVRRRVGGGYFALMTTHPDGPFAGGKPTAGLVAFRCRDA
                     HLAVGYSG"
BASE COUNT          659 a         1503 c         1462 g          774 t
ORIGIN      
        1 atccggttcg ccatggccga ggggcgtcgc ggcgcgttca ccccgaagtc gctcgtcgta
       61 gggcatgact cgtttaaggg catgtccatc gagttcggag atctcagcgg caccggcaag
      121 ttcgacatgt tcgtcagcaa catcaccaca ccgtggggcc tggaggagag caacttcgtc
      181 tggcacaaca cggcccgcag tccggccgag gcgcgcagca agctgagcca gggtgtcgca
      241 ccgttcgaca accgggccgc ggcgatgaac ctcgcgtggg tcggctgggg ctgggacgcg
      301 aagatggccg acttcgacaa cagcggcgtc atgtcgatcg tgcagacgtg cggcttcgtc
      361 aaggggaaca tcaaccggtg gaactggctg caggagctcg cgatgagcaa cgacgtgctg
      421 ctcgaggagc cgaacatgtg gcccaaggcg gaggtcggtg acgacatcgc cggctcccag
      481 ccgatcgcgt tctgggtgcg cgaggacaac ggccgatacg tcaacctcag caccgatctc
      541 ggcttcgact cgaccaccac cccgacccgc ggcgtggccg tcggagacgt gaacgccgac
      601 ggcgggcagg acttcgccgt ggcgcgtcag tggggtccgc cggccttcta ccgcaacggc
      661 aacgtcggtc gggacaacta catcgggctg cggctctacc ggccagcggt cggcggcacc
      721 gccggagccg atgctccgat cggcgcgccc gcctacggcg ctcaggcccg gatcatcacc
      781 gccgacgggc ggacccaggt cgcccaggtc gacggaggcg gcgggcactc gggcaagcgg
      841 agcttcgaca tcttcttcgg tctcggaaag gccggcgacc ggccggcgtc ggtcgagctg
      901 atgtggcgcg acaacgacgg tatggcccac cgccagacgt tcgacctgat ggccggctgg
      961 cacaacttcg ttctcacaga ccaggcccag gaggtgacgt catgacgacc gacgtccgct
     1021 acgccggcgg acctgacacc aggacggtca caaccggccc gaacgggctg cgcgaccagg
     1081 tgaagccgcc gacggtggcg cgcgcgccgg cggtcccccg tgtcgacccg cgcgacccgc
     1141 gttacctcgc cctgcgcaac ttcgcgatat ccatcagcgt cttcaacatc ctcggctaca
     1201 ccgtgctcgg gttcgagcag ccgtggacgt ggcccttctt cgcgctcgcc gtcgggtact
     1261 cgatcgagat cgcgatcgag ctcatcgcgg ctcgcgccca tcgccgcccg ccggcgttcg
     1321 tcggcaacgg gatgtggggc ctttacacgt tcctgctgcc gacccacatc acggcactgg
     1381 ccgccaacat gctgctctat gccaacaccg acttctggcc catcgcgttc gcggtggcgg
     1441 tggccatcgg gcagaaagcg gtcatggtgg cacccatcaa gggtcggatg cgccacttca
     1501 tgaacccgtc gaacttcggg atcacggtga cgctggtctg cttctcgtgg gtgaacatcg
     1561 cgccgccata ccacttcacg gagcacgtac ccgacgtcat ccgcatcatg gtgccgatca
     1621 tcatcatcac ggcaggcacc gtgctcaacg cgctgctcac caagaaggtg ccgctgatcg
     1681 tcgggtgggt cggggcgttc gtgatccagg cgctgatccg ctggtggatc tgggacgtgc
     1741 acctgtgggc ggccctcgcc ccgatgacgg gcgtggcatt cgtcctgttc accaactaca
     1801 tgatcaccga ccccggcacg acgccgagct cgggtcgatc catgttcatg ttcggatcaa
     1861 tcgtcggcac cgtctacggc gtgttgatgg tattcaacgt ggtgtatacg ctgttctttg
     1921 ccgtcaccat cgtgtgcctc ggtcgcggtc tactgtggtg gtggaagtcg ctgcgcgaac
     1981 ggcgtagccc atatacaccg gtcgggcgtc caggactgct ggcgcccgcc agcccgtccg
     2041 ccggatgagc atcccgggcg gcggcaccgc acaacggcct cgccgttgcg gtgccgccgc
     2101 ccgggtcgcc gttcggcggc gattgatcgg ccgtccgaac cggcagatca accgtccgga
     2161 actgtcagcc gccgttggaa ccgacatgct gggggaatcg tgagcactca tcaggcgtcc
     2221 gcgcttctgc tcgggctcgc tgccatcatc ctcggcgctg gtctcctcgg atgggtggca
     2281 cgcaagctcg ggcagccggc cgttatcggc gaggttatcg ccggcatcct gatgggaccg
     2341 acgctgttcg gcggcgcgat cgccggcttc ctgctgccga cggacatccg accgttcctc
     2401 agcgctctgg ccaacgtcgg cgtcgccgtg ttcatgttcc tggtcggcct ggagctcaac
     2461 cgcagcatgt tgcggggtac cggtcgggtc gcggcgaccg tgtcgctcac ctcgatcctg
     2521 ttgccctgca cgctgggcat cctgctgggc acgtaccttc tgcgcaccca cccgagcaag
     2581 gaccggctcg gcttcgtgct gttcatgggt gtggccatgt cgatcacggc gttcccggtg
     2641 ctggcccgca ttctcatcga ccgcgaactg aaccggctgc cactcggcgg tctcgccctg
     2701 acctgtgcgg ccatcgacga cgtgctggcg tggtcgttgc tcgcggtcgt cgtgacggtg
     2761 ctgggcggag acgcggacca gtggcggatg ctgctggccg tgccgtacct gctactcatg
     2821 ctctacccgg tgcggcggct gctcgggcgg ttggtctccg cccgccaacg caccgggcgg
     2881 ctcaccacgg gccagatagc aatagtgctc gccggcctgc tggtctccgg tggcgtgacc
     2941 gagtggatcg gcctgcactt catcttcggc gcgttcctgt tcggtctggt catgcctcgg
     3001 ggcgagaagg ccgagctgcg gacggagttg cacgatcgga tcggtctggt caacaacgtg
     3061 ctcctcctgc cggtcttctt catcgtcgcc ggcctgcggg tcgatctgtc gaaggtggac
     3121 gcggtcgacc tggcggagct gggcctcatc ctgctcgtcg ccatcggcgg caagttcata
     3181 ggtgcgttca tcgccgcccg tctcaacggg ctgggcaacc ggatcgccgc caccctggcg
     3241 acgctgatga acacgcgcgg cctcaccgag ctgatcattc tcagtgtcgg cctgcagctg
     3301 ggcgtcctcg acaccggcct ctactcgctg atggtggtga tggcggtggt gaccacggcg
     3361 atggccggtc cgctgctgtc catcctctac ccgcgccgtc tggtcgcccg tgacatcgcc
     3421 gcgaccgagg cggccgtgca cgcggccgaa ccggggctgc atcccttgtg gatcggcggt
     3481 cgcgggccga tatcctcggc ttcgccgcgg agctgacgga catacgacgg ggcgagactt
     3541 cttacggttc ggttaagcac tcccagagaa gcagcgtcgg ggcgcgcgta cgacgattaa
     3601 tgcgggcgga ccaatcggac aaggtgcgac gctcgccagc gatccgcgcc gggattggcc
     3661 gggccccatc cgggaacggg cagccgcgaa tcagccgatg acctcaggac tggtgaccga
     3721 cgtgaccatc gcgatcgacc tgaagccccc agccgatctg cttccgcgac agccgcaacc
     3781 acacgccctg ccgccgttgc tggagcggag cgacctgtgg accgacttca tggtggccga
     3841 cgccaggatc ccgatgtacg acgtgccgtt cgtctcgatc ggcggcggca tgggctcctt
     3901 catgacggtc gactacctgc gtgctgccgg gggcatgccg gcgcgcgaca tccgggtact
     3961 gtccaatctc acccatccgt ggcagaccta cgagaagctg gccaccgcct cgcagatctc
     4021 ccgcgcgcgc cgcatccgct ccgacgcggc gtcgcggccc gacaacctgt gggggttccc
     4081 ctccttcgcc ctgcaggagg cgttgaagga ccgcacgatc aagccgctca tccaggtgtt
     4141 gctggagccc gtgttcgccg acttctacac gccgaagctc ggtgacgtgc tcggtggcat
     4201 ggagcgcgaa gcgaaccgga tcggctactg ggacatgctg gtgcgcggcg acgtgcagat
     4261 cgtgcggcgt cgcgtcggcg gtggctactt cgccctcatg acgacccacc ccgacggtcc
     4321 cttcgccggt ggcaagccga cggccggtct ggtcgcgttc cggtgccggg acgcccatct
     4381 cgccgtcggc tactcggg
//
LOCUS       DASRJA010000441         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1454050,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000441 DASRJA010000000
VERSION     DASRJA010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454050"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /gene="secY"
                     /locus_tag="VFC01_19305"
     CDS             complement(<1..1199)
                     /gene="secY"
                     /locus_tag="VFC01_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZN81809.1"
                     /translation="MLSAFISSLRTVDLRRKILFTLGIVILYRVGASIPSPGVNYPNV
                     QKCVAQVSGGDSAQIYSLINLFSGGALLHLTVFAVGVMPYITASIIVQLLTVVIPRFE
                     QLRKEGQAGQAKMTQYTRYLAVALAILQATSIVALAANGGLLQGCTLDIIQGQSEGLN
                     IFTLVVIVLVMTAGAALVMWMGELVTERGIGNGMSLMIFAGIAARIPIEGKTILDSRG
                     GMVFTLVCVAALIIIIGVVFVEQGQRRIPVQYAKRMVGRRMYGGTSTYLPLKVNQAGV
                     IPVIFASSLLYLPDLVSRLTQSASSTPSWWQTFIQSYVADQGSWVHIMLYFGLIVFFT
                     YFYVAITFNPDERADEMKKYGGFIPGIRPGRPTAEYLRFVLSRITLPGSIYLGTIAVL
                     PNLFLDIT"
     gene            1477..>2043
                     /locus_tag="VFC01_19310"
     CDS             1477..>2043
                     /locus_tag="VFC01_19310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN81810.1"
                     /translation="MARTENDTWDLASSVGATATMVAAARAMASTAANPLINDPFAEP
                     LVRAVGVDFFTRLVTGELRPEDLDSDTESVGMQRMTDNMAVRTKFFDEFFLGATDAGI
                     RQAVILASGLDSRAYRLEWPAGTTVYEIDQPEVIEFKTRTLGELGASPTADRRTVAMD
                     LRYDWPSALIEEGFDPRQPTAWSAEGLLG"
BASE COUNT          379 a          635 c          691 g          338 t
ORIGIN      
        1 gtgatgtcca ggaacaggtt cggcagcacc gcaatggtgc ccaggtagat cgagccgggg
       61 agggtgatcc ggctgagcac gaaccgcagg tactccgcgg tcggtcggcc cggccggatg
      121 ccggggatga agccgccgta cttcttcatc tcgtcggcgc gctcgtcggg gttgaacgtg
      181 atcgcgacgt agaagtaggt gaagaacacg atgaggccga agtacagcat gatgtgcacc
      241 cagctgccct ggtcggccac gtagctctgg atgaaggtct gccaccacga cggggtcgac
      301 gacgccgact gggtcaggcg cgagaccagg tccggcaggt agagcagcga ggacgcgaag
      361 atcaccggga tgacgccggc ctggttgacc ttgagcggca gatacgtcga cgtgccgccg
      421 tacatgcgtc gacccaccat gcgcttggcg tactgcaccg ggatgcgccg ctggccctgc
      481 tcgacgaaca ccacgccgat gatgatgatc agcgcggcca cacagaccag cgtgaacacc
      541 atgccgccgc ggctgtccaa gatggtcttg ccttcgatcg ggatgcgtgc ggcaatgccg
      601 gcgaagatca tcagcgacat gccgttgccg atgccgcgct cggtgaccag ctcgcccatc
      661 cacatcacca gcgccgcgcc cgcggtcatc acgagcacga tgacgaccag cgtgaagatg
      721 ttcaggccct cgctctggcc ctggatgatg tcgagggtgc agccctgcag cagcccgcca
      781 ttcgcggcca gcgcgacgat gctggtggcc tgcaggatcg ccagtgcgac cgcaaggtag
      841 cgggtgtact gggtcatctt ggcctggccc gcttgccctt ctttgcgcag ctgctcgaag
      901 cgcgggatga ccacggtcag cagctgcacg atgatgctcg cggtgatgta gggcatcacg
      961 ccgaccgcga acaccgtcag gtgcagcagc gcacccccgg agaacaggtt gatcaacgag
     1021 tagatctgcg ccgagtcgcc accgctgacc tgcgccacgc acttctgcac gttcgggtag
     1081 ttgacgcccg gcgacgggat cgaggcgccc acccggtaca ggatgacgat gcccagggtg
     1141 aacaggatct ttcgcctcag gtcgactgtc ctgagtgaag agataaaggc cgagagcact
     1201 cttcctcctg cgcagccggc ctatagatcg cggcgtgcga atggggctgg tatgaccagc
     1261 gtcttggtta agtcctgcgc ggaccgccga caagcgcaca gcctgcggag tcacgcgtca
     1321 aacagtctac gagactaaca gttgaggctc gtacggcccg catccgttgc aggtaactgc
     1381 gcggaagccg gctttcagaa tcggagcgta cagtcggcgg cgggtcgtta catcgcctaa
     1441 gtaacggccg tgtactacgt cgataggggt cgagggatgg cacgcacgga gaacgacacg
     1501 tgggacttgg catcgagcgt cggggcgacg gccaccatgg tggcggcggc gcgcgcgatg
     1561 gccagcactg ccgctaatcc gttgatcaac gatccgttcg ccgagccgct ggtgcgcgcg
     1621 gtcggagtgg atttcttcac ccgcctggtc actggtgagc tgcgacccga ggacctcgac
     1681 agcgacaccg agtccgtcgg catgcagcgg atgaccgaca acatggcggt gcgcaccaag
     1741 ttcttcgatg agttctttct cggcgcgacc gacgcgggca tccggcaggc ggtgattctg
     1801 gcgtcgggcc ttgattcgcg cgcttaccgg ctcgagtggc cggccgggac gacggtgtat
     1861 gagatcgacc agcccgaggt catcgagttc aagaccagga cgcttggcga actgggcgcc
     1921 tcgccaaccg cggaccgccg cacggtcgcg atggatctgc gctacgactg gccgtcggcc
     1981 ctgatcgaag agggcttcga cccgcgtcaa cccacggcct ggagtgcgga gggactgctc
     2041 ggt
//
LOCUS       DASRJA010000442         6584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3015905,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000442 DASRJA010000000
VERSION     DASRJA010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3015905"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VFC01_19315"
     CDS             complement(<1..410)
                     /locus_tag="VFC01_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HZN81811.1"
                     /translation="MPSKTAARAGARSSRSKAGSRGGSRPAPRRKPARRHNPSAISSA
                     GATVGRGVRAGWLMVAKGAGSTARSVGRARDLEPGHRRDGIALALLGVAVVIAASSWF
                     DAARPVGQWIDTSLRVLIGSAVVLVPIVLVGVAVV"
     gene            571..885
                     /locus_tag="VFC01_19320"
     CDS             571..885
                     /locus_tag="VFC01_19320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZN81812.1"
                     /translation="MPVTVVATMKAKPESVDAVRDACKQAIEAVHSEPGCDLYSLHEA
                     DGTFVFVEQWADADALKTHSTAPAVATLFGTVGDLLDGAPDIKMLQPVVAGDPAKGQL
                     RP"
     gene            885..1703
                     /locus_tag="VFC01_19325"
     CDS             885..1703
                     /locus_tag="VFC01_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZN81813.1"
                     /translation="MASLAGKVAFITGAARGQGRAEAVRLASDGANIIAVDICDQIAS
                     VPYPLATADDLAATVKLVEETGARIVAQQADVRDQESLAAALQAGLDDFGRLDIVVAN
                     AGIAPMESGAKGWQDVIDVNLTGVHHTVEVAKDTMVKQGEGGSIVLTSSSAGLVGIGG
                     GDQGSLGYTAAKHGVVGLMRAYANHLAPHSIRVNSIHPSGVNTAMIDNDHTRQWLANV
                     IANSQRPPDMGNAMPVQVLEPEDIAAAVAWLVSDEARYITGVALPVDAGYVNKR"
     gene            1706..2578
                     /locus_tag="VFC01_19330"
     CDS             1706..2578
                     /locus_tag="VFC01_19330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZN81814.1"
                     /translation="MARNPAAQTAFGPMVQAAIEQYEPPERRLVFDDLALPILPAGQR
                     ALVRAMRWPLLRRLTIAAGERAVPGSWALIACRKRYIDDKLDEALGDIDAVVVLGAGM
                     DTRAYRLAHRSDIPVFEVDLPINIERKKAALRRAIGGVPASVHLVPLDFEHDDLIGTL
                     TGHGYRTDARTFFVWEGVTQYLTEDAVRATLGALQAAPTGTRLVFTYVRKDFIDGVNM
                     YGAAILYKRFRQRQQVWRFGLDPDEAAEFVEEYGWRLLEQAGPDYFLSHYIRPTGRNL
                     GASQLEWSAYAEKR"
     gene            2681..3574
                     /locus_tag="VFC01_19335"
     CDS             2681..3574
                     /locus_tag="VFC01_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003408272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81815.1"
                     /translation="MTKVAPRRVIDVLMSPPAPQAKSGHIALTGLRLLAGLMWLENVV
                     WKVPPDFGERTRGGLYFWTTRAVEFPVWKPYSWLLEHLVLPNFTLFGWGVFVIESALV
                     VLLLTGTAVRLAALLGVAQSIAIGLSVAEAPNEWPWAYAMMIGIHAVLVFAPSAQYAA
                     VDALWSGTATTARRLLGGWGIALGLIALVALCVYLRYDRRANVGILDLEFSLGDYNLW
                     GTLLLIGVAVAMLAAAMFGWRLAALVAGGVALAGAASIYVQVGRTDVWLGGTPSTAAV
                     FACGAVVGLAAGLKLAQSKGA"
     gene            3574..4689
                     /locus_tag="VFC01_19340"
     CDS             3574..4689
                     /locus_tag="VFC01_19340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN81816.1"
                     /translation="MSSSQRLNEVLTVAARHAEQVDSEGAFPAEAVEALRASGILGLV
                     LPREIGGLGAGPVEFTEFVADLAAACGSTAMIYLMHVSAAVAVAAAPPAGMPNLLADM
                     ASGQSLGTLAFSEKGSRSHFWAPVSASSAEGDDVTIKADKSFATSAGFADVYITSTGS
                     PGGEGVDVYAIPADTPGLEPSGTFSGMGLRGNASAPMSIDVCIPASYRVGAEASGFAL
                     MMDTILPWFNLGNAAVSVGLAASATTSAINHVGNARLEHLGSSLAELPTIRAQIARMG
                     IALAAQRAYLTVAANSVSSPSDATLTHVLGVKASANDAALAITESAMRVCGGAAFSKQ
                     LPIERVFRDARAGSVMAPTADVLYDFYGKAVTGLPLF"
     gene            4698..5522
                     /locus_tag="VFC01_19345"
     CDS             4698..5522
                     /locus_tag="VFC01_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HZN81817.1"
                     /translation="MPKQQLVVGAVAYTPNVVPIWEGIRSYFQESENPDGQMDFVLYS
                     NYARLVDSLIAGHIDIAWNTNLAYVRTVLQTDGHCTALAQRDSDVDFTTVFVARDGSG
                     LAGAADIAGKRLALGSADSAHAAILPLYYLRRAGVDDTDLQITRFDSDIGKHGDTGRS
                     ELDAVEAVLAGDAEVAAIGSSTWAAMGAEELMGGALTEVWRTDGYCHCMFTALDTLPA
                     ERYDPWLDRLLAMSWDNPEHRKILEMEGLRRWVRPHLDGYQPLFEALEEQGVDPRW"
     gene            5519..6514
                     /locus_tag="VFC01_19350"
     CDS             5519..6514
                     /locus_tag="VFC01_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZN81818.1"
                     /translation="MILDLMRLMGDLAQGATGEIEIRSPADRDLTERWCARNGNTVVR
                     ADVNASGVGTLVIRRGHAPDPVNVLGADRLPGVRLWMYTNFHCNLACDYCCVASSPQT
                     PRRELGADRVHRLVDEAAAWGVRELFLTGGEPFLLPDIGTIIADCVKRLPTTVLTNAM
                     VFKGRARRALEDLPRELLALQISLDSATPELHDRHRGAGSWAKAVDGIRLALSLGFRV
                     RVAATVAAPAPGELAAFHDFLDGLGILRDDQLVRPIALEGVAAEGVSISRESLVPEVT
                     VTADGVYWHPVAATDERALVTRTIEPLTPALDTVARLFAEQWARSAEAAAVFPCA"
BASE COUNT         1095 a         2149 c         2206 g         1134 t
ORIGIN      
        1 accaccgcga cgccgacgag cacgatggga acgaggacga cggccgaccc gatcagcacg
       61 cgaagggagg tgtcgatcca ctggccgacg ggacgagcgg cgtcgaacca ggagctggcg
      121 gcgatcacca cggcgacgcc aagcagtgcc agtgcgatcc cgtcgcggcg atgtcccggc
      181 tcgaggtcgc gggcgcggcc gaccgaccgt gcggttgacc cggccccctt ggccaccatc
      241 aaccagccgg cgcgcacacc gcgtccgacg gtcgcccccg cggacgagat ggccgacgga
      301 ttgtggcgtc gagccggctt ccggcggggc gccgggcggg acccgccccg cgaaccggcc
      361 tttgacctgc tcgaacgggc accggctcgg gcggcggtct tactaggcat gcactcaagc
      421 ctagtcgcac aggccccata atcaccatct gccacacggg tcacagaaag gtctcaattg
      481 gacccggcct ggagccgaca tgggctgacc gaagcggaat ctcataggta ggctaggcaa
      541 ccgtccagca cgcactcatc gaggagtccc atgcccgtca ccgtcgtcgc aaccatgaaa
      601 gccaagcccg agtcggtgga cgccgtccgc gatgcgtgta agcaggccat cgaggcggtg
      661 cattccgaac ccggttgcga tctctactcg ctgcacgagg ccgacggcac cttcgtcttc
      721 gtcgagcagt gggccgacgc ggacgcactg aagacgcaca gcacggcacc cgcggtcgcc
      781 acgctgttcg gcaccgtcgg tgaccttctc gacggtgcac cggacatcaa gatgctgcag
      841 cccgtcgtcg cgggtgatcc ggctaagggc cagctgcgcc cctgatggct tccctcgcag
      901 gcaaagtcgc attcatcacg ggtgctgcgc gcggtcaggg ccgagcggaa gcggttcggc
      961 tggcgtccga cggcgcgaac atcatcgccg tcgacatctg cgatcagatc gcctcggtgc
     1021 cgtatccgtt ggctacggcc gacgatctgg ccgcgacggt caagctcgtc gaagagacgg
     1081 gtgcgcgaat tgtggcacag caagccgacg ttcgcgatca ggagtcgctg gccgcggctt
     1141 tgcaggccgg gctggacgac ttcggccggc tcgacatcgt cgtcgccaac gccggtatcg
     1201 caccgatgga atcgggtgcc aagggctggc aagacgtcat cgacgtcaac ctcaccggtg
     1261 tgcaccatac ggtggaagtc gccaaggaca cgatggtcaa gcagggtgag ggcggatcga
     1321 tcgtcctgac cagctcctca gcgggtcttg tcggcatcgg aggcggcgac caaggctcgc
     1381 tcggctacac ggcggcaaaa cacggcgtcg tcggattgat gcgagcctat gccaatcacc
     1441 ttgcaccgca tagtattcgc gtcaactcga tccatcccag cggcgtcaac acagcgatga
     1501 tcgacaatga ccacacccgg cagtggctag cgaacgtgat cgccaattcg caacggccgc
     1561 cggacatggg taatgcgatg ccggttcagg tcctcgagcc ggaggacatt gccgcagcgg
     1621 tggcctggct ggtatccgat gaggcgcgct atatcaccgg cgtggcactt ccggtggacg
     1681 cgggctacgt caacaaacgc tgatcatggc gcgaaatccg gccgcgcaga ccgcctttgg
     1741 tccgatggtc caggcggcca tcgagcagta cgagccgcct gaacgccgtc tggtcttcga
     1801 cgacctggcc ctgccgatct tgcccgccgg tcaacgggcg ctcgtgcgcg cgatgcgctg
     1861 gccactgctg cgccggctga ccatcgcagc gggcgagcgg gccgtgcccg gctcgtgggc
     1921 gctcatcgcg tgccgcaagc gctacatcga cgacaagctc gacgaggccc tgggagacat
     1981 cgatgccgtc gtcgttctcg gtgcgggcat ggataccagg gcgtatcgcc tcgctcatcg
     2041 atccgacatt ccggtcttcg aggtcgacct cccaatcaac atcgagcgta agaaggccgc
     2101 cctgcggcgc gcgatcggcg gggtgcccgc ttcggttcac ctggtgccct tggactttga
     2161 gcacgacgac ctgatcggca cgctaaccgg gcacggctac cgcaccgacg cccgcacctt
     2221 cttcgtttgg gaaggtgtga cgcagtacct gaccgaggac gcggtgcggg ccacgctcgg
     2281 cgcactgcag gccgccccga ccgggacccg gctggtgttc acctatgtcc gcaaggactt
     2341 catcgacggg gtgaacatgt acggcgctgc catcttgtac aagcgtttcc ggcaacggca
     2401 gcaggtttgg cgtttcggtc tcgatcccga cgaggctgcg gaattcgtgg aagagtacgg
     2461 gtggcggctc ctcgagcagg cgggccctga ttacttcttg agccactaca ttcggccgac
     2521 gggccgcaat ctgggcgcgt cgcagctcga gtggtcggcg tacgcggaga aacggtgacc
     2581 tgacaaccgc cccgaggcgg tgatggggcc gaagcccgcg ccgtcgctgc gtttggcgaa
     2641 caccaggaca ccgcgccctg ctggctgtga gacttaaccc atgaccaagg tcgcccctcg
     2701 tcgggtgatc gacgtgctga tgtcgccgcc ggcgccgcag gcgaagagcg gtcacatcgc
     2761 cctgacggga ctgcgactgt tggccggctt gatgtggctg gagaacgtgg tgtggaaggt
     2821 gccaccggac ttcggcgagc gaactcgcgg gggcctgtat ttctggacca cccgcgccgt
     2881 tgagtttccg gtatggaagc cgtacagctg gctactcgaa cacctggtgc tgcccaactt
     2941 cacgctgttc gggtggggcg tgttcgttat cgagtctgct ttggttgtgc tgctgctgac
     3001 gggcaccgcc gtgcggctgg cggccctgct cggtgtcgcg cagtcgatcg cgatcggcct
     3061 gtcggtggcg gaggcgccca acgagtggcc gtgggcctac gcgatgatga tcggcattca
     3121 cgcggtcttg gtgttcgcgc cgtccgcgca atacgccgca gtcgatgcgc tgtggtcagg
     3181 gaccgccacc accgcccgac ggctactcgg cggttgggga atcgcgttgg gattgatagc
     3241 ccttgtcgcc ctgtgcgttt accttcgcta cgaccgtcgg gccaacgtgg gcatattgga
     3301 tctggagttc tcgctcggcg actacaacct gtgggggaca ctgctactca tcggggttgc
     3361 cgtggccatg ctcgcggcgg cgatgttcgg ttggcggctg gccgcgctgg tcgccggtgg
     3421 cgtcgctttg gctggcgcgg cctcaatcta tgtgcaagtc ggacgtaccg acgtgtggct
     3481 cggtggaacg ccgagcactg cagcggtttt cgcctgcggc gcggtcgtgg ggctagcggc
     3541 ggggctcaag ctggcacagt cgaaaggagc gtaatgtcgt catctcagag gttgaacgag
     3601 gttcttacgg tagcggcgcg gcacgccgaa caggttgact ccgagggtgc ctttccagcc
     3661 gaagcggtcg aggcgctaag ggccagtggc attctcggcc tggtactgcc gcgagaaatc
     3721 ggcggcttgg gcgcaggccc agtcgagttc accgagttcg tggccgacct cgctgccgcg
     3781 tgcggttcga cggccatgat ctacctcatg cacgtgtccg cggccgtcgc cgtcgccgcg
     3841 gcgcccccgg ccggtatgcc gaatctgttg gcggacatgg cctccggaca atcgctcggc
     3901 acgctggcgt tcagcgagaa gggatcccga tctcatttct gggcacccgt ttccgcatcg
     3961 tcggccgagg gcgatgacgt aacgatcaag gccgacaaga gcttcgcgac gtcggcagga
     4021 ttcgccgatg tctacatcac atccactggt tcccccggcg gcgagggcgt cgatgtatat
     4081 gcgatccccg cggatacgcc tggcctcgaa ccgtcaggca cgttcagcgg catgggcctg
     4141 cgcggaaacg cctccgcccc aatgtcgatc gatgtctgca ttccggcttc ataccgggtc
     4201 ggcgccgagg caagcggttt cgctctgatg atggacacca tcctgccgtg gttcaacctc
     4261 ggcaacgccg ccgtgtcagt tggattggcc gctagcgcca ccacctcggc gatcaaccac
     4321 gtgggaaacg cccggctcga gcacctcggg tcaagccttg ccgagcttcc tacgatccgc
     4381 gcccagatcg ctcggatggg catcgcgctc gccgcacaga gggcgtatct aaccgtggcc
     4441 gccaacagtg tgagttcccc gagcgatgcg acgctgacac atgtactggg cgtgaaagcc
     4501 tcggcgaacg acgctgcgct tgccatcacc gaatcggcca tgcgggtatg cggcggcgcg
     4561 gcgttctcca agcagctacc catcgaacgc gtattccgcg acgctcgtgc ggggtcggtc
     4621 atggccccca ccgccgacgt gctgtacgac ttctacggca aagccgtgac cggtctgccg
     4681 ttgttctagg aggggacatg cctaaacagc agttggtcgt cggggcggtc gcatacacac
     4741 cgaacgtcgt gccgatctgg gaggggattc gaagctactt tcaggaatcc gaaaacccag
     4801 acgggcagat ggatttcgtc ttgtactcga actacgcgcg gctggtcgac tcgctgattg
     4861 ccggccacat cgacatcgcg tggaacacca acctcgccta tgtgcgcacc gtcctgcaga
     4921 cggacgggca ctgcaccgct cttgcgcaac gcgactccga cgtcgacttc accacagtgt
     4981 tcgtcgcgcg cgacggcagc ggcctggccg gggcagctga tatcgccggc aaacgacttg
     5041 cgctgggttc ggccgactcg gcccacgctg cgatcctgcc gttgtattac ctgcgacggg
     5101 ccggcgtgga cgacacagac cttcagatca cccgcttcga tagcgacatc ggcaagcacg
     5161 gcgacaccgg ccgcagcgag ctcgacgcgg tggaggccgt gcttgcgggt gacgcggaag
     5221 tggccgcgat cggcagctcg acgtgggcag cgatgggcgc cgaggagctg atggggggtg
     5281 cgctgacgga ggtgtggcgc accgatggct attgccactg catgttcacc gccctggaca
     5341 ccttgcccgc agagcgatac gacccctggc tggatcgctt gctagcgatg agttgggaca
     5401 atccggaaca ccgaaagatc ctggaaatgg agggcttacg acgctgggtg cggccacacc
     5461 tggacggcta ccagccgttg ttcgaagctc ttgaagagca gggcgtcgat ccgcgatggt
     5521 gatcctcgac ctgatgcgct tgatgggcga cctcgcgcag ggtgccacgg gcgagatcga
     5581 gatccgctcc cctgccgacc gcgacctcac cgagcggtgg tgcgcgcgca acggcaacac
     5641 ggtggtgcgc gccgacgtga acgcctccgg cgtcggaacg ctcgtaatac gccgcgggca
     5701 tgcaccggat cccgtcaacg tgctcggcgc cgaccggcta cccggcgtgc ggttgtggat
     5761 gtacaccaac ttccactgca accttgcctg cgactactgc tgcgtcgcgt cgtcgccaca
     5821 gacgccgcgg cgtgaactcg gcgcggatcg cgtccaccgg ctcgtggacg aggccgctgc
     5881 gtggggggtg cgcgagctgt tcctcacggg cggcgagccg ttcctgctgc ccgacatcgg
     5941 cacgatcatc gccgactgtg taaagcggct gcccacaact gttctcacca acgcgatggt
     6001 gttcaagggg agggcgcggc gcgcactcga agatctgccg cgggagctgc tcgcgctgca
     6061 gatcagcctc gactcggcaa cacccgagct gcatgaccgc caccgcgggg cgggcagttg
     6121 ggccaaggcc gtcgacggca tccgactggc gctgtctctt ggcttcagag tgcgcgtggc
     6181 cgcgacggtg gctgcccccg cgccgggaga gcttgcggcg ttccacgact tcctcgacgg
     6241 ccttggcatt ctccgcgacg atcaactcgt ccgacccatc gcgctggaag gcgttgcggc
     6301 cgaaggggtg tcgatctctc gcgagtcact tgttccggag gtgacggtca ccgctgacgg
     6361 cgtgtactgg catccagtgg ccgccaccga cgaacgcgcc ctggtgaccc gcaccattga
     6421 gccgttgacg ccggcgctgg acaccgtcgc ccggctcttc gccgagcagt gggcgcggtc
     6481 ggccgaagcc gctgcggttt tcccgtgcgc atagcggcct attgctgcac gttggcgacg
     6541 agccacctgt gcacaccgac gaaggtgcgc caatggccaa gcac
//
LOCUS       DASRJA010000443         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1396114,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000443 DASRJA010000000
VERSION     DASRJA010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396114"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VFC01_19355"
     CDS             <1..1067
                     /locus_tag="VFC01_19355"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZN81819.1"
                     /translation="HLPDAARTLIEYGLADTTPAVVTANGTTCQQRSVETTLAGLLDK
                     NTLAGTEPTGPLAGPLVATIGKTVANRAKLNWWESRALYGWTVLVPRTKDQAGEMSEK
                     LISHGAVPIEVPTIAVEPPRSPAQMERAVKGLVDGRFQWVVFTSTNAVRAVWEKFNEF
                     GLDARAFSGVKIACVGQATADRVRAFGINPELVPSGEQSSLGLLDEFPPYDDVFDPVN
                     RVLLPRADIATETLAEGLRERGWEIEDVTAYRTVRAAPPPASTREMIKTGGFDAVCFT
                     SSSTVRNLVGIAGKPHARTIVACIGPKTAETAAEFGLRVDVQPDVAAVGPLVEALAEH
                     AARLRAEGALPPPRKKSRRR"
     gene            1086..2066
                     /gene="hemB"
                     /locus_tag="VFC01_19360"
     CDS             1086..2066
                     /gene="hemB"
                     /locus_tag="VFC01_19360"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZN81820.1"
                     /translation="MSFPSHRPRRLRSTPALRRLVAQTSLEPRHLVLPMFVAEGIDEP
                     REISSMPGVVQHTRDSLRSAAERAVAAGVGGLMLFGVPREEDKDATGSAGVAEDGILN
                     VALRDLTKDLGDDTVLMADTCLDEFTDHGHCGVLDAAGRVDNDATNARYVELAVAQAE
                     SGAHVVGPSGMMDGQVAAIRDGLDAAGHTDTVILAYAAKFASGFYGPFREAVGSSLRG
                     DRRTYQQDTANAREAVHEIELDIDEGADIVMVKPAMSYLDVVRAAADISPVPVAAYQV
                     SGEYAMISAAAANGWIDLQTLALETLTSIRRAGADIVLTYWAADVARWLA"
     gene            2063..>2443
                     /locus_tag="VFC01_19365"
     CDS             2063..>2443
                     /locus_tag="VFC01_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81821.1"
                     /translation="MSDPQARPLDVDTGFWLWVAALPLMVIGYVVDVVVTGASHSPMI
                     AYAISAVFVVVLSAVVMTFMILMRQGYRWARTVLTGGGIASVVYTGTNLFTVDRPPAA
                     AVAYAVTAIIGAVLIVGGVYLLHRK"
BASE COUNT          389 a          801 c          864 g          389 t
ORIGIN      
        1 ctcacctgcc cgacgcggcg cgcacactga tcgagtacgg gctggccgac accacgccgg
       61 ccgtcgtgac cgcgaatggc acgacatgcc aacagcgttc ggtggagacc acgctggccg
      121 gcctgctcga caagaacaca ctagcgggca cagaaccgac gggcccattg gcggggccgc
      181 tggtggcgac catcggcaag accgtcgcca accgagccaa gctgaactgg tgggagagcc
      241 gcgcccttta cggctggacc gtgctggtgc ctcgcaccaa ggatcaggcc ggcgagatga
      301 gcgaaaagct gatcagccat ggcgcagtgc cgatcgaggt gccgacgatc gccgtcgagc
      361 cgcctcgcag ccccgcccag atggagcgtg ccgtcaaggg tttggtcgac ggccggttcc
      421 agtgggtggt gttcacctcc accaacgcgg tgcgcgcggt gtgggagaag ttcaacgagt
      481 tcggtctcga cgcccgcgca ttctcgggtg tcaagatcgc ctgcgtagga caagccaccg
      541 ccgaccgggt acgcgccttc ggtatcaacc ccgagctggt gccgtcgggc gagcagtcgt
      601 cgctgggcct gctcgacgaa ttcccgccct atgacgacgt tttcgacccg gtgaaccggg
      661 tgctgctgcc gcgggccgac atcgcaaccg agacgctggc cgagggtctg cgcgaacggg
      721 gttgggagat cgaggacgtc accgcctacc gcacggtgcg cgcggcgccg ccgcccgcgt
      781 cgacccgcga gatgatcaaa accggtggct tcgacgcggt ctgcttcacc tccagctcga
      841 cggtgcgcaa cctcgtgggt atcgcgggga agccgcacgc gcggaccatc gtcgcgtgca
      901 tcggcccgaa aactgctgaa acggcagcag aattcgggct gcgcgtcgat gtgcagccgg
      961 acgtcgccgc ggtcggtccg ctggtcgagg cgctggccga gcatgccgcg cggctgcgtg
     1021 ccgagggcgc gctgccgcct ccgcgcaaga agagccgccg gcgatagatc aaaggatggc
     1081 gttgcatgtc ctttcctagc catcgccccc gtcggctgag gtcgactccc gcgttgcgtc
     1141 gcctcgtcgc gcaaacatcg ttggagccaa ggcatttggt gctgccgatg ttcgtcgccg
     1201 agggcatcga cgagccgcgt gagatcagct cgatgccggg cgtcgtgcag cacacccgtg
     1261 actcgctgcg ctccgccgcg gaacgcgcgg tggccgcggg cgtcggcggg ctcatgctgt
     1321 tcggggtgcc gcgcgaagag gacaaggacg ccaccggatc ggcgggcgtg gccgaggacg
     1381 gcatcctcaa cgtcgcgctg cgcgatctga ccaaggacct cggcgacgac accgtgttga
     1441 tggccgacac ctgcctcgac gagttcaccg atcacggcca ctgcggcgta cttgacgccg
     1501 cgggccgcgt cgataacgac gccaccaacg cccgatacgt ggaattggct gtggcgcaag
     1561 cagaatcggg cgcccacgtg gtgggtccga gcggaatgat ggacggtcag gtcgcggcga
     1621 tccgcgacgg ccttgacgcc gccggccaca ccgacacggt gatcctggcc tacgccgcca
     1681 agttcgcatc tggtttctac ggcccgttcc gcgaggccgt cggctccagc ctgcgcggcg
     1741 accggcgcac gtatcagcag gacacggcca acgcccgcga ggcagtgcac gaaatcgagc
     1801 tggacatcga cgagggcgcc gacatcgtca tggtcaagcc cgcgatgagc tatctcgacg
     1861 tggtgcgtgc ggccgcggac atctcaccgg tgccggtggc cgcgtatcag gtctccggcg
     1921 aatacgcgat gatcagcgcc gccgccgcca acggctggat cgatctgcag acgctggcgc
     1981 tggagacgtt gaccagtatc cggcgcgcgg gcgccgacat cgtgctgacg tactgggcgg
     2041 ccgacgtcgc ccgctggctc gcgtgagcga tccccaagcc cggccgctcg acgtcgacac
     2101 gggattctgg ctgtgggtgg ctgcgctgcc gctgatggtg atcgggtacg tcgtcgacgt
     2161 cgtggtgacc ggggcgagtc attcgccgat gatcgcgtac gcgatctctg cagtgttcgt
     2221 ggtcgtgctg tcggcggtcg tgatgacgtt catgattctg atgcggcagg gctaccggtg
     2281 ggcccgcacg gtgttgacag gcggcggcat cgcgtccgtg gtgtacacgg ggaccaattt
     2341 gttcacggtg gaccggccgc cggcggcagc ggtcgcgtac gcggtcaccg cgatcatcgg
     2401 ggcggtgctg atcgtgggcg gtgtgtacct gttgcatcgc aag
//
LOCUS       DASRJA010000444         5893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_981260,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000444 DASRJA010000000
VERSION     DASRJA010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981260"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFC01_19370"
     CDS             <1..218
                     /locus_tag="VFC01_19370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN81822.1"
                     /translation="VGVDPAAQGRGLGATLTLTGLHHLAQQLSGADEPMVMLYTEADN
                     TAAVKTYERLGFTVFTADVAYAVTESR"
     gene            complement(313..990)
                     /locus_tag="VFC01_19375"
     CDS             complement(313..990)
                     /locus_tag="VFC01_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HZN81823.1"
                     /translation="MSAELNRDVVTRSSIFAALADPGRLAIVDRLLVGDVSPTEIQLL
                     MSMPSNLVAHHVRVLQKAGIARRVRSEGDRRRTYLRVNTDTLEAIVPSVARKTQRVVF
                     VCTQNSARSQLAAAIWNRRRSVPAASAGTHPAAEMHPEALALARRRNLPMEPRTPRHL
                     DDVVTPDDLIIAVCDNAHEELPTTLSRIHWSISDPARSSLPDAFDRAFDELTQRIDYL
                     APRLQPV"
     gene            1229..2368
                     /gene="pstS"
                     /locus_tag="VFC01_19380"
     CDS             1229..2368
                     /gene="pstS"
                     /locus_tag="VFC01_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HZN81824.1"
                     /translation="MKHNRIGAAVGILAAGALLLSACGSDENKSSTGATTTGNGTAGD
                     SAAKVDCAGKKDLKASGSSAQANAVTRFVTAYETACSGYTLNYTSSGSGAGVSEFLGG
                     QTDFGGTDSALNAEKGEVEKAKQRCGGADAWNLPTVFGPIAVTYNLPGVDGVALDGPT
                     LAKIFNGAVTTWNAPEIAALNSGKTLPGDKINVVFRSDESGTTDNFQKYLKAASDGAW
                     TQEAGKTFNGGVGEGAKGNEGTSAAIKNTPGSITYNEWSYATKQGLQVADIVTAAGPD
                     PVKLSVESAGKSIDSVKIKGQGNDLVIDTSSFYKPTAAGAYPIILPTYEVVCSKYPDA
                     EVASAVKAFLTVATTDGQKGLQEAGYIPVPDTFKAKLTEAINAIS"
     gene            2462..3427
                     /gene="pstC"
                     /locus_tag="VFC01_19385"
     CDS             2462..3427
                     /gene="pstC"
                     /locus_tag="VFC01_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZN81825.1"
                     /translation="MSTQPPPASALKGHRRWGDAIFYSIAVAAGATIIGAIALMGLFL
                     LIRAFPSLAADNANFITSSEFVTTDENNLRFGIADLFRVTVLSSVFALAIAVPIAIGI
                     ATFLTNYVPRRFSRPFAILVDLLAAVPSIVFGLWGIFVLAPKLEPVQRFLNENLNWIF
                     LFGDGNVSLAGGGTIFTAGVVLAVMILPIITSVSREVFSLTPKGHVEAAQALGATKWE
                     VIRMTVYPYGRSGMIAGSMLALGRALGETVAVLIILRTAAQAGHWSLFDGGYTFASKI
                     ASAAAEFSAPLPTGAYIAAGFVLFALTFVVNALARLAAGGRVSGG"
     gene            3424..4326
                     /gene="pstA"
                     /locus_tag="VFC01_19390"
     CDS             3424..4326
                     /gene="pstA"
                     /locus_tag="VFC01_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZN81826.1"
                     /translation="MTDTLDAPVKAPTFHAISTSRKIKNRVAEALFAAAFLIAMVPLV
                     WLLYTVISRGYEAVISSTWWNRSLAGVLPEEFAGGVYHAIYGTIIQATVAAVLAVPLG
                     IMAAIYLVEYGRGWFARTVTFMVDILAGVPSIVAALFIFALWIATMGFPQSALAVSLA
                     LVLLMLPVVVRNTEEMLKLVPDELREASYALGIPKWKTIGRIVVPTALPGIISGILLA
                     LARVIGETAPVLVLVGYARSINFDPFDGNMASLPLLIYTELINPEEAGRLRVWGAALT
                     LILIVAALYIAAAFVIRFLTRNRT"
     gene            4343..5119
                     /gene="pstB"
                     /locus_tag="VFC01_19395"
     CDS             4343..5119
                     /gene="pstB"
                     /locus_tag="VFC01_19395"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZN81827.1"
                     /translation="MAKRIDIKDLNIYYGSFHAVADVGLSVPPRSVTAFIGPSGCGKS
                     TVLRTLNRMHEVLPGARAEGSVLLDGEDIYGAGVDPVSVRKTIGMVFQRPNPFPTMSI
                     RDNVVAGLRLQGVRNKKTLDEVAERSLKGANLWNEVKDRLDKPGGGLSGGQQQRLCIA
                     RAIAVQPAVLLMDEPCSALDPISTLAIEDLISELKKDYTIVIVTHNMQQAARVSDQTA
                     FFNLEATGKPGRLIEIDDTEKIFSNPTQKSTEDYISGRFG"
     gene            complement(5133..5807)
                     /locus_tag="VFC01_19400"
     CDS             complement(5133..5807)
                     /locus_tag="VFC01_19400"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011724187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HZN81828.1"
                     /translation="MTESPAAHAVHHHPDVSIDQDLALRAAAARLQEEFGDQIGMDTI
                     EKLLHSSYDHFAARARIPNFVPLLAERFARQQLHAVARIEGNVGGGAPTVLFLCTHNA
                     GRSQMALGFFTHLAGEQAVAWSGGSEPETEINPAAVEAMGEVGIDITGEYPKPWTDEI
                     VQAADVVITMGCGDSCPVFPGRRYEEWELSDPAGQNVESVRLIRDEIEKRVRRLLAEL
                     GVPATA"
BASE COUNT         1075 a         1818 c         1885 g         1115 t
ORIGIN      
        1 tcgtcggcgt cgacccggcc gcgcagggcc gagggctcgg cgcgacgctc accttgaccg
       61 ggctccacca cctcgcccag caactgtcgg gcgcggacga gccgatggtg atgctctaca
      121 ccgaagccga caacaccgcc gcagtgaaaa cctatgagcg tcttggattc acggtgttca
      181 ccgccgatgt ggcgtatgcc gtgactgaat cgcgctagcc gcggcacgca ctcgccgtct
      241 tgagcaagag gcgctgatcg atcgacaggt cagtggtggt gttgtcgcac atcagtcagt
      301 cctgctttct tattagacgg gttgcaatcg tggcgcgaga tagtcgatgc gttgggttag
      361 ctcatcgaag gctcggtcaa acgcgtcggg cagcgagctt cgtgcaggat ccgatatcga
      421 ccaatgaatg cgtgacaggg tcgtgggcaa ctcctcgtgg gcgttgtcgc agaccgcaat
      481 gatcaagtcg tcgggggtga cgacgtcatc gagatgacgc ggtgtccgcg gctccatcgg
      541 gagattgcgg cgccgtgcca aagccagtgc ctcgggatgc atctcggccg ccggatgagt
      601 acctgctgac gccgcgggca ccgagcgtcg acgattccag atcgcggctg ccagttgact
      661 gcgcgcagaa ttctgggtgc acacgaacac cacccgctgt gtcttacgtg ccacagaagg
      721 gacgatcgcc tcaagtgtgt cggtgttgac tcggagatac gtgcgccgcc gatcaccctc
      781 cgagcggaca cgtcgcgcga tgccggcttt ctgtagtacc cgcacgtgat gggcgaccaa
      841 attcgagggc atggacatca gcagctgaat ttcggtcggc gaaacgtcac caaccagcag
      901 acggtcgaca atcgctagcc gcccagggtc agcaagcgcg gcgaagattg acgacctcgt
      961 cacgacatcg cgatttaact cagcagacat tgactcaatg atgactgaga tagttaaaaa
     1021 gatcaacgtt gttcgcgtaa ttcgactccg cgtccccgta tccggttcat ctcccgttca
     1081 tcaatgcgtg gatgcgcgtt cacagggcac ccctaggttc gtgagccgag cagcgatcgg
     1141 atctgctcgg cggaaccggg gttccgtcgc ttgcccgaac tccgggcggc gggtcacgtc
     1201 cgtcgtccct cacaattaga ggagatacgt gaagcacaac cgaatcggcg ccgcggtggg
     1261 cattttggcc gcgggtgccc tgctgctttc ggcctgcggc agcgacgaga acaagagcag
     1321 caccggcgca acgacgaccg gcaatggaac tgcgggagac agcgccgcca aggtcgactg
     1381 cgccggcaag aaggacctga aggccagcgg ctcgtctgcg caggccaacg ccgtgacccg
     1441 gttcgtgacc gcctatgaaa cggcctgctc gggttacacc ctgaactaca cctccagcgg
     1501 atccggtgcg ggtgtctccg aattcctcgg cggccaaacc gatttcggtg gcaccgattc
     1561 ggcgctgaac gccgagaagg gcgaggtcga aaaggccaag cagcgctgcg gcggtgcgga
     1621 cgcgtggaac ttgccgacag tgttcggtcc gatcgcggtc acgtacaacc tgccgggcgt
     1681 cgacggcgtg gctctcgatg gtcccaccct ggccaagatc ttcaacggag cggtcacgac
     1741 gtggaacgcg cccgagatcg cggcgctcaa ctccggcaag accctgccgg gtgacaagat
     1801 caacgtggtc ttccgcagcg acgagtccgg caccacggac aacttccaga agtatctcaa
     1861 ggcggcgtcg gacggagcct ggactcagga agccggcaag accttcaacg gcggcgttgg
     1921 tgaaggcgcc aagggcaacg agggcacgtc cgccgcgatc aagaacaccc cgggttcgat
     1981 cacgtacaac gagtggtcct acgcgaccaa acagggcctg caggtggcgg acatcgtcac
     2041 cgcggccggt cctgatccgg tgaagctgtc ggtcgagagc gctggtaagt cgatcgattc
     2101 ggtcaagatc aagggccagg gcaatgacct cgtgatcgac acgtcgtcgt tctacaagcc
     2161 gactgcggcc ggcgcgtacc cgatcatcct gccgacctac gaggtcgtct gctccaagta
     2221 cccggatgcg gaggttgcct cggccgtcaa ggcgttcctg acggtcgcga cgaccgacgg
     2281 ccagaagggc ttgcaggagg ccggctacat cccggtcccc gacacgttca aggccaagct
     2341 gaccgaagcc atcaacgcaa tttcctagtc aaaggcagca atgactttga ggcctgaccc
     2401 gacggctgtg ggctcgtcaa caaagggggc gagcccacag cagatcgggg atactccaga
     2461 catgtcgacg caaccaccgc cggcatcggc cctgaagggc catcgccggt ggggcgacgc
     2521 aattttctac tcgatcgccg tcgcggcggg cgcgacaatc atcggcgcga tcgcgctgat
     2581 gggtcttttc ctgttgatcc gcgcgtttcc gtcgctggcg gcggacaacg ccaacttcat
     2641 caccagttcg gaattcgtca cgaccgacga gaacaacctg cgcttcggta ttgccgatct
     2701 cttccgcgtc acggtgctca gttcggtctt cgcacttgcg atcgcggtac cgatcgccat
     2761 cggcatcgcg acgttcttga cgaactacgt cccacgccgg ttctcccggc cgttcgccat
     2821 cttggtcgac ctcctggccg ccgtgccatc gatcgtcttt ggcttgtggg gcattttcgt
     2881 cctggctccc aagctcgagc cggtccaacg gttccttaat gagaacctga attggatctt
     2941 cctcttcggc gacggcaatg tatcgctcgc gggcggcggc acgatcttca ccgccggcgt
     3001 tgtgctcgcc gtgatgatcc tgccgatcat cacgtcggtt tcgcgggagg ttttcagcct
     3061 gacccccaag ggtcatgtcg aggcggccca agcgctgggt gcgaccaagt gggaagtcat
     3121 ccggatgacg gtctacccgt atggccgcag cggaatgatt gccggctcga tgctggcatt
     3181 gggtcgggca ctcggtgaga cggtcgcggt cctgatcatt ctgcgcactg cagcgcaggc
     3241 tggacactgg tcgctgtttg acggcggcta cacgttcgcg tccaagatcg cttcggctgc
     3301 agcggaattc agcgcgccgc tgcccaccgg tgcgtacatc gcggcgggct tcgtcctgtt
     3361 cgccctgaca ttcgtcgtca acgcgcttgc tcgattggca gctggaggaa gggtgagcgg
     3421 aggatgacgg acacgcttga tgcgcctgtg aaggcgccga cctttcacgc gatcagcacc
     3481 agccgcaaga tcaagaatcg tgtggcggaa gcactgttcg cagcggcgtt cctgatcgcc
     3541 atggtgccgt tggtttggct gctctacacc gtgatctcgc gcggatatga ggcggtcatc
     3601 tcgtcgacgt ggtggaacag gtcgttggcc ggggtgcttc cggaagagtt cgccggcggc
     3661 gtgtaccacg cgatctacgg cacgatcatc caggcgacgg tagcggcggt gctggcggta
     3721 ccccttggca tcatggccgc catctatctg gtcgagtacg gccgcggatg gtttgcgcgg
     3781 acggtgacgt tcatggtcga catcctggcc ggtgtgccgt cgatcgtcgc ggcgctgttc
     3841 attttcgcgc tgtggatcgc cacgatgggc tttccgcaga gcgctcttgc ggtgtcgctg
     3901 gcgttggtgc tgctgatgct tccagtcgtc gtccgcaaca ccgaggaaat gctcaagttg
     3961 gttcccgacg agctacgtga agcttcctac gcgctgggca ttccgaaatg gaagaccatc
     4021 gggcgcatcg tcgtgcccac tgcactgcca ggcatcatca gcggaatcct gctggcactc
     4081 gcgcgggtga tcggcgagac ggcaccggtg ctggtgctgg tcggctacgc caggtcgatc
     4141 aacttcgacc cattcgacgg caacatggca tcactgccgt tgttgatcta cacggaactg
     4201 atcaatcccg aagaggcggg acggctgcgg gtgtggggtg ccgcgcttac gttgatcctc
     4261 atcgtggccg cgctatacat cgccgccgcg ttcgtgatcc ggttccttac ccgaaaccga
     4321 acttgacgaa ctaggagcgg atgtggccaa gcgcatcgac atcaaagacc tcaacatcta
     4381 ttacggctcg tttcacgccg tagcggacgt ggggttgtcc gttccgccgc gcagcgtgac
     4441 ggccttcatc ggtccgtcgg gctgtggtaa gtcgaccgtg ctgcgcacgc tcaaccggat
     4501 gcacgaggtg ctacccggcg ccagggccga gggctcggtg ctgcttgacg gcgaggacat
     4561 ctacggcgcc ggcgtcgatc cggtgagtgt ccgcaagacc atcggcatgg tattccagcg
     4621 gccaaatccg tttcccacca tgtcgattcg cgacaacgtg gtcgcgggtc tgaggctgca
     4681 gggcgtacgc aacaagaaga cgctcgacga ggtcgctgag cgctcgctca agggtgccaa
     4741 tctgtggaac gaggtcaagg accggctgga caagcccggt ggcgggctgt cgggcggtca
     4801 gcagcagcgg ctgtgcatcg cgcgcgctat cgctgtgcag cccgccgtgt tgctgatgga
     4861 cgagccgtgc tcggcgctgg accccatttc cacgctggcg atcgaggatt tgatctcgga
     4921 gctgaagaag gactacacga tcgtcatcgt cacgcacaac atgcagcagg ccgcccgggt
     4981 gagcgaccag accgcgttct tcaatctcga ggccaccggt aagccgggca ggctcatcga
     5041 gatcgacgac accgagaaga tcttctccaa cccgacccag aagtccaccg aggattacat
     5101 ctcgggtcgc ttcggctgaa agtcagccgg gttcaggccg tggcggggac gcccagttcg
     5161 gcgagcaacc gacggacgcg tttctcgatc tcgtcgcgga tcagtcgaac cgactccacg
     5221 ttttggccgg cggggtcgga cagttcccat tcctcgtagc gcctaccggg aaacactggg
     5281 cagctgtcgc cacagcccat cgtgatgacc acgtcggcgg cttgcacgat ctcgtcagtc
     5341 caaggtttgg ggtactcacc ggtgatgtcg atgccgacct cacccatcgc ttcgaccgct
     5401 gccggattga tttcagtttc cggctcggag ccgccggacc acgccaccgc ctgctcgccg
     5461 gccaggtggg tgaagaaccc cagcgccatc tgcgagcggc cggcgttgtg agtgcacagg
     5521 aaaagcaccg tcggcgcgcc gccgccgacg tttccctcga ttcgggcgac tgcgtgtagt
     5581 tgctggcgtg cgaatcgttc ggccagcagc ggcacaaagt ttggtatccg cgcccgcgcg
     5641 gcgaagtggt cgtaggacga gtgcagcagc ttttcgatgg tgtccatccc gatttggtcg
     5701 ccgaactcct cctgcaaccg ggccgcggcg gccctcagcg cgagatcctg atcgatggat
     5761 acgtccgggt ggtgatggac tgcgtgggcg gcggggcttt cagtcatcgc gaactcctgt
     5821 cggcgtatcg gtcagcaact ctgacttcga cggatacccg atatttgcga atatcaaagg
     5881 aaggcacaag cga
//
LOCUS       DASRJA010000445        31602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_71156,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000445 DASRJA010000000
VERSION     DASRJA010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 31602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31602
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71156"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VFC01_19405"
     CDS             <1..1076
                     /locus_tag="VFC01_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN81829.1"
                     /translation="GKTNTPEWGNHCTTEPSLFGPTVNPWSASITPGGSSGGSAAAVA
                     AGVVPAASGGDGTGSIRVPASCCGLVGLKPRRGRTSFAPGAGHGLEGLVNEHALTRTV
                     RDSAALLDAVAGSATGDPYTAPVPNVPFLQAITHQPAATRILIATASPYPGPATHPEV
                     VAAVEQTGTVLARLGHVVEPGAPTIDADAVADAIAVLHNVSNAQLHALATAHLGREPH
                     EDEFEPSTWVMVREGFTTSGVAYAEAIAAVHAQTRRFVAGMANHDVLLVPTLLTPPPP
                     YALLDQPRGTTRAFFDVEFATTGWTSLANVTGSAAISLPLGVTSNGLPIGVQLMAPDE
                     TILLQLAAQLETAMPWLHRRPSM"
     gene            1086..1937
                     /locus_tag="VFC01_19410"
     CDS             1086..1937
                     /locus_tag="VFC01_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81830.1"
                     /translation="MVRDIRELTDSPQAYAEELRRRWGGLLSYRYIGRSYASMDLGPE
                     DNTVTIRHDMRNSTGGLLLAVLCIAAPEGGHMSDLEAVPNPVIHSCQILDPGRDVRRI
                     EIVSEDLKRGRQMGYSRAKIVDADRPSRVLALTQGQGVSIGVPPEGLERMEANPLEVI
                     DSLDLPPLWQVFGGHRRPDGHWALPELAVEVASPDAALHIGPQFVIGETAAADAAAAL
                     AGTDSLQAISSHVMFVARGKVGPFRVETEPMPGADGTLAVRTITHDEGVEDRITTVGS
                     YLFRRAD"
     gene            1959..2873
                     /locus_tag="VFC01_19415"
     CDS             1959..2873
                     /locus_tag="VFC01_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN81831.1"
                     /translation="MAVSIARPKLEGNIAVGDDRQIGFAEFGDPQGRAVFWLHGTPGA
                     RRQIPMEARVYAEQREIRLIGVDRPGIGSSTPYQYDTVFAFADDLHTIADTLGIDKFE
                     VIGLSGGGPYTLACAAAMPDRVVAAGVLGGVAPVRGADAIGGGVSTLISAAAPVVELA
                     GAPLRLAASTLIRLIRPVAEPALYAYARISPEADRQLLVRPEFKAMFLDDLLNGSRKQ
                     LAAPFADVVVFARDWGFRLEEVKVPVRWWHGDRDHIVPFEHGQHVVSKLPDAQLTHLP
                     GESHLAGLGRAEEILGTMVELWDRADQK"
     gene            complement(2874..3659)
                     /locus_tag="VFC01_19420"
     CDS             complement(2874..3659)
                     /locus_tag="VFC01_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN81832.1"
                     /translation="MRIDRRSFAAAVTLGSAAALIGAQRASAAPPPDPMPQARNVVLV
                     HGAYADGSCWSDVIPYLQARGLNVASVQNPLRTLDEDCEFARRTLALMDGPTVLACHS
                     YSGMIATEVGVDPKVTALVYIAARAPDAGEDYTALAAQYPAPPASAGLVKAPDGYAQL
                     SESAFLNDFAQDVPAARARVLYTVQGRISDELFNGRTTQAAWRDKPTFYAVSKNDRTI
                     NPDLERFMAQRMNAKTIELDASHLSIVSKPREVGDLIIEAAGG"
     gene            complement(3732..4943)
                     /locus_tag="VFC01_19425"
     CDS             complement(3732..4943)
                     /locus_tag="VFC01_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced intracellular survival protein Eis"
                     /protein_id="HZN81833.1"
                     /translation="MLASDTSTSVEIRSAADADWAAISLLDSTSFGVFGHPEALAAWR
                     TLMPTDSSVIACDGNNVVGMAHYLDMRLTVPGGAELPMAGVTIVAVAPTHRRRGVLRS
                     MYTELHNRIADARYPIAGLTASEGGIYGRFGYGPATIEQEFSVDRRFARFRDDAPDPG
                     GVRIVKPADHRDDLEEIYERWRRQTPGGVVRPKALWDELLADREWVRRGGTELFAFLH
                     PDGYALYRVFGDDPMIVRVGELVPATTDAGIALCRALFGLDLMEKVVFGTHPADPLPY
                     LLADPRVARLTHYEDDLWLRIMDIPTTLEARTYEADVSIVIEISDGFRSDGGRFALKI
                     RDGRARCAPTDAEADVRMDLDVLGSLYMGAHKASSYAAANRLHTNDSALLGRLDAAFV
                     SDVPAVLGYGF"
     gene            5036..5410
                     /locus_tag="VFC01_19430"
     CDS             5036..5410
                     /locus_tag="VFC01_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZN81834.1"
                     /translation="MATDLTLYLDDHPGELARVGDALGKAGTNIAGLCAVTTGGGVAE
                     VHILVQDATSAFEALQDAGIKIAAEQEVIVLDVEDRPGALGEVARKLADAKVNLTTAY
                     LATNTRLVLAADNLAEAKSALT"
     gene            complement(5411..5635)
                     /locus_tag="VFC01_19435"
     CDS             complement(5411..5635)
                     /locus_tag="VFC01_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81835.1"
                     /translation="MTATDERQTLAELAEEGGWHRRNVDRTDYYQKAGARVQVIWQGT
                     EVISGGTLYHDDVLTTYSRDLSTVKGWLRR"
     gene            5682..6176
                     /locus_tag="VFC01_19440"
     CDS             5682..6176
                     /locus_tag="VFC01_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HZN81836.1"
                     /translation="MMALEVQQADVTRLEVDAITNAANTELKHGGGVAGAISRAGGPA
                     VQRESTEKAPIGLGEAVETTAGDMPARYVIHAATMELGGPTSADIIAQATRSTLAKAD
                     ELGCRSLALVAFGTGVGGFALDEAARLMVDAARGHKPRSLERVVFAVHGDEAERAFRT
                     ALEQ"
     gene            6173..7276
                     /locus_tag="VFC01_19445"
     CDS             6173..7276
                     /locus_tag="VFC01_19445"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HZN81837.1"
                     /translation="MTDGTPTVGVEEEFLLIDPDTRQPQPAAAAVVTRAAPGLGDLVS
                     GEFTRCQIEIKTPPCSEAGRLRTELAQLRTAAAGAAASEGLRLCASGTPPLAGREPSA
                     VGDHPRYRAGVAQYRSMLDDFMVCSLHVHVHLPDRELAVRVSNHLRPWLPLLIAVSAN
                     SPFHQGNDTGYADWRAVVRSRFPCLGPPPYAESLRHHEELATAIAESGAMLDAATPFW
                     DIRPNPRLPTLEVRTMDVVADIDDTVALAMVIRALVATATDKALAGDPGPQPSSEVLR
                     AAYWRAARDGWPGCGVDALTQEMLPAPVQTARLLDHVAPALRRHGDSDIVHRFVDRLT
                     ERGTGADLQRASLARHGSLTGVVDDLVTLTART"
     gene            complement(7254..7964)
                     /locus_tag="VFC01_19450"
     CDS             complement(7254..7964)
                     /locus_tag="VFC01_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HZN81838.1"
                     /translation="MTPEAAAQQLTRVGTGLIEPAELKACLDIDASEWTRFAAHWEDL
                     VPDPYAAELGTRRLRRYGHFRFTPAEAIAEPMPHDAFVQPENSNPLYVGKDRQFEPLT
                     DAFAKDPLLERILKLLGQLAMALDDTPTWSAKVTPFRVLASADDDGMPTPEGMHRDGV
                     TLVTSLLIGRRNAIGGKSTVTDIDGRHMLTTTLDEPGTLLLGDDRRTLHGVSPIRPCD
                     PAEPARRDVLVITFAPSA"
     gene            8072..8428
                     /locus_tag="VFC01_19455"
     CDS             8072..8428
                     /locus_tag="VFC01_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81839.1"
                     /translation="MKITKNKIVAAALGAAAAPALLFAGAGTAQAATAWAWPDSDPLG
                     VTMHIQSTGAWGWCEYTAVPVGGGIKPLPAYKVPFYLNNGQESTLWFPGIPTGTTWNV
                     TITCEHGGLNNSQVLY"
     gene            complement(8422..9696)
                     /locus_tag="VFC01_19460"
     CDS             complement(8422..9696)
                     /locus_tag="VFC01_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HZN81840.1"
                     /translation="MSARAGIVVTGTEVLTGRVQDRNGPWIADRLLELGVELAHITIC
                     GDRPDDIEAQLRFLADEGVDIIVTSGGLGPTADDMTVATVAKFAGLELVLDAALEDRI
                     TDILKRLMARFDNVDIDAVRAANRKQAMVPVGAHAINPVGTAPGVVVPGKPTIVVLPG
                     PPRELQPMWPVAVETEAVQRAIAGRTTYRQEMVRMFGLPESGLADTLRDGEQSIEGFD
                     RLEITTCLRRGELEIVTRYEPDAAGIYAKLLELLRQRHGREIFSEDGTRVDDQIAQLL
                     AGRRIATAESCTGGLLAARLSDLAGSSAYVAGGVVSYANEAKTDLLGVAADLIAEHGA
                     VSEPVAEAMADGALGRFGADTAIAITGIAGPGGGTDEKPVGTVCFTVKLADGETFTRT
                     LRLPGDRSDIRERSTTVAMHMLRRALTGWRAQ"
     gene            9736..10122
                     /locus_tag="VFC01_19465"
     CDS             9736..10122
                     /locus_tag="VFC01_19465"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN81841.1"
                     /translation="MTAPKSLRELFDQLGLQEVPSADGTVTMEMPVDERTVNTAGGLQ
                     GGLVATMADVTAGQLAARSTPFGMRIATTDLFIRYLRPIKVGPARAVARILRTGKRSV
                     VVQVDIYRANDDEVGATATVNFAAVD"
     gene            complement(10100..12886)
                     /locus_tag="VFC01_19470"
     CDS             complement(10100..12886)
                     /locus_tag="VFC01_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HZN81842.1"
                     /translation="MSGYRRRHIVLRILLSVALLLLLCAADFPDFAKPGPSPGQIELA
                     QGWSLVSARNVPADGAVLSKPDYQAAGWHAIPRMPATVLQTLQEDGTYPDLYYGTNLG
                     AVPQDLYKQDWWYRTTFTAPSGHTTYLLEFPGINYRAEIWLNGHLIAGNTQIVGMHTA
                     HELDVSRWVNQGGSNALAVKITPERALQDIDGVELADNWYDWINWNYLGYQGPGKNPA
                     NGNSFVPDRNAGIWKPVYLKVSGAVVLGSSTVTSELPLPRTNSAKLTIYSNLRNTSAQ
                     QLRGVLRATITRPGKPDVQIEQPVTLAAGEQREISFTPDTFAQLTVRNPDLWWPYTLG
                     RPDLYDLQLEFRQYNRPIGTSHQRFGIRSVQQFRDQDEQFLELGKGGNFYLKVNGKDF
                     LVRGAAYTPDLLYANDPSRDAAILGYVKDLGLNMIRLEGKFPGDHIAEMADEMGIPLM
                     YGWMCCNQWEKWNQWDGEDNRVAQDSLRSQIEGLRSHPSVFIWANGSDGKPPAEVLAK
                     YHAILSDLHWPNAIVDTVSSLATDAAGERDWDGIQMAGPYSWRPPSYWFAGRYAATRG
                     STAEQGDNEQIPPFASLKKFIPPDKLWPINDAWFFHAGSNSKNAALTSIRRAVDRRYG
                     VSTGAEEFARKAQLAHYESTRAQFEAFAAGGWDTHKMTIYWMLNNHWPSFFGHIFDYY
                     LRPGGAYFGAKKGLRPLSVVFDSYATGNHDQANITVVNQTPREQTGLTVRVRVYDLQG
                     RVRDDRSAGNIYVTPGGTTQALTLPRLARDSSVFFVRAQLLDWAGKVVSENVYWQSQR
                     PDDVGDPRNDSAFELTQTSWADMTGLNYMPQVPLDVTASRAANSGDNRVTIRLRNSSS
                     RVAFFERAELVSTPEDDEILPVEYSDNYVTVFPGETVELQGQPWTGATANWVRVTGYN
                     TPPVVVPVSPPPRS"
     gene            complement(12966..14309)
                     /locus_tag="VFC01_19475"
     CDS             complement(12966..14309)
                     /locus_tag="VFC01_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HZN81843.1"
                     /translation="MHEVDQTTEQMVRSVLAYAENRLRMNPVPLDKGVLPADELHDRL
                     DGVIREQPRQPDEVLGVYTSVIAPSVISADSPRFLGFIPAAPTKAALLFDMLVSCASI
                     QGISWLEASGAIAAENSVLRVIANEAGLPTTSGGCFVSGGSAGNLSALAVARETAKKR
                     GATGRLRVVVGSDAHSSIVNTLRLLEMDALVVDTPDHRLTADTVREAVPPDAANIAAV
                     VCTSGTTNAGIIDDLAGVGALAKERGWWFHVDGAYGGSGIFARSLRPKYNGLEQADSF
                     ILDPHKWLFTPFDCCALLYRDPELARGTHTQDASYLDVIHTSDGEWNPSDYAYHLTRR
                     ARGLPLWFSMAVYGLEAYRGAIEVAASLAVQTAELIKSAPQLELIREPDLGVVLFRRL
                     GWKPEDYDAWAQRLHEDQVAFIPPTKWEGETVGRFAFLHPDTTLELVREVLARTE"
     gene            complement(14359..15096)
                     /locus_tag="VFC01_19480"
     CDS             complement(14359..15096)
                     /locus_tag="VFC01_19480"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZN81844.1"
                     /translation="MGRVDGKVALISGGARGMGAEHARALVAEGAKVVIGDILDDEGK
                     ALADEIGEAARYVHLDVTEADEWDAAVATAVNEFGKLNVLVNNAGIVALGQIGKFDMA
                     KWQKVIDVNLTGTFLGMQASVEAMKEAGGGSIINVSSIEGLRGAVMVHPYVASKWAVR
                     GLTKSAALELGSHQIRVNSIHPGFIRTPMTKHFPDNMLTIPLGRPGQSDEVATFVVFL
                     ASDESRYATGAEFVMDGGLVNDVPHRI"
     gene            15162..16235
                     /locus_tag="VFC01_19485"
     CDS             15162..16235
                     /locus_tag="VFC01_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HZN81845.1"
                     /translation="MAIRVALIGTGNCGSLALKQLVNDARFDLTGVWVSSDAKVGKDA
                     GELAGLGVTTGVTATDDLDAIIAVKPDCIVYCAMGDTRLPDAMTEVRKILAAGIDVVG
                     SGLGVLQYPWQVIPDKYIHRVEDAAREGNSTVFMTGVDPGFATDLLPFALAGTCQRIE
                     QVRTMEIADYATYDGATVMFDVMGFGNEIGDLPMLYQPGVLSIAWGTAIRQLAAGLGI
                     EVDEIKDCVEQEPAPEDFEVAVGTIKKGTVAAVRFQIEGMVNGKPVIVVEHVTRLRGD
                     LRPDWAQPAQPGGSYRVEITGEPSYTMDVCPTSRNGDHNYAAILAAAGRIVNAIPDVV
                     AARPGIRTTLDMPLVTGRGVYAG"
     gene            16251..17015
                     /locus_tag="VFC01_19490"
     CDS             16251..17015
                     /locus_tag="VFC01_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HZN81846.1"
                     /translation="MDPHASPDLVAEIGAGPEGPGVGAFFDLDGTLVDGFTATAHASD
                     RIRRRQARIGEVLGVIEASMRYRFGRMQFERLLTRAAGYLRGESLAALDVVGERLFVE
                     RVSSRVFPYMHEVVRAHQQRGHTVVLSSSALTIHAEPVARFLEINHVLCNHFELDEHG
                     LLTGRIAKPVIWGTRKAAAVQQFCDANGVDLQRSYFYADGNEDAALMKLVGQPRPVNP
                     RPRLAAIAAENGWPVLQVTGSSGRRRLTLRRLVGYD"
     gene            17177..17440
                     /locus_tag="VFC01_19495"
     CDS             17177..17440
                     /locus_tag="VFC01_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81847.1"
                     /translation="MQLSRLGAVTAAAAIAFGALLSATAPIANAEKISETTIKSECKS
                     AGGTYTSVVKGGTRFSTCDYKDNEGNGSRDYYANGEYYSTRPT"
     gene            17499..18356
                     /locus_tag="VFC01_19500"
     CDS             17499..18356
                     /locus_tag="VFC01_19500"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN81848.1"
                     /translation="MLYPDQPIGVLIERIAWLESLGFDQVFLPDHSGNLRNRRGVWFD
                     SWAVLAAAAVTTSRVRLGVMVANQILRPPALLAKQAVTLDHMANGRFELGIGAGIFAW
                     DHLSVGEQPWPPRERALRFKDYAAIVDGLLHGSGAPFSYAGERLWVDDVIPAPTPLQS
                     PRLPIIVGGQPPTVIRVAAEHADVWNTHGRPGAAADEVLTATAEQSRRLDELALAAGR
                     DPASIRRSYTIFGEWDPRAGRYTYEDVFDSFGAIGISDFVLDWPDDDYVDTFEAAARE
                     VLPARRRGA"
     gene            18409..19542
                     /locus_tag="VFC01_19505"
     CDS             18409..19542
                     /locus_tag="VFC01_19505"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HZN81849.1"
                     /translation="MHTPICDELNIEFPIFAFTHCRDVVVAVSKAGGFGVLGAVGFTP
                     EQLEIELNWIDENIGDHPYGVDIVIPNKYEGMDATDLTPEVLKKQLNALVPQEHIDFA
                     KKILADHGVPVDHSDDDALQLLGWTEATATPQVEVALKHPKCTLIANALGTPPADMIK
                     HIHDEGRKVAALCGSPSQARKHADAGVDLIIAQGGEAGGHCGEVGSIVLWPQVVKEVA
                     PVPVLAAGGIGSGQQIAAALALGAQGAWTGSQWVMVEESEHTEVQHAAYAKATSRDTV
                     RSRSFTGKPARMLRNDWTEAWENPDNPKPLGMPLQYMVSGMAVAATHKYPHESVDVAF
                     NPIGQVVGQFTKVEKSSAVIERWVQEYLEATNTLNELNEAATA"
     gene            19569..19994
                     /locus_tag="VFC01_19510"
     CDS             19569..19994
                     /locus_tag="VFC01_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81850.1"
                     /translation="MAVLWMDLIFDAQVLRHRGAHELPESVLDSISAYYHRATTTSRP
                     MSYLIALVMLTLLGSLGFRWILGDDATWVLTASTVLAAVPILLAFVRTVPNAVRLGAR
                     AGSPAELSHLARSICRDHFICLTFMSAFLVLWAVTASPT"
     gene            20087..20680
                     /locus_tag="VFC01_19515"
     CDS             20087..20680
                     /locus_tag="VFC01_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN81851.1"
                     /translation="MPSPRERMVISAALLIRERGAQPTAIADVLEHSGAPRGSAYHYF
                     PGGRTQLLCEAIDYSGEYVAAKIAKADSGLDALEGLVAYYRKQLLRSDYRAGCPVVAV
                     TVEAGDPAKQDQATPVIDRAAAAFTRWTDLIAQRLVADGVSADRADELAMLSTTAIEG
                     AIIVARASRDLKPLDLVHGQLRDMVAAEMSERKPRDD"
     gene            20673..22949
                     /locus_tag="VFC01_19520"
     CDS             20673..22949
                     /locus_tag="VFC01_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN81852.1"
                     /translation="MTSRDVAAEAAPQDWQQTACILCECNCGIVVQVDGRNLAKIRGD
                     KSHPASQGYTCNKALRLDHYQNNGNRLTSPMRKRPDGSYQEIDWDTAISEIAAGFKRI
                     ADTHGGDKIFYYGGGGQGNHLGGAYSGAFLKVLGSHYRSNALAQEKTGEAWVDAHLYG
                     GHTRGEFEHAEVSVFIGKNPWMSQSFPRARTVLNEIAKDPGRSMIVIDPVITDTAKMA
                     DIHLRVRPGTDAWCLAALAAVLAQENLCNEAFLAEHVNGVEPVRDALRDVPIGEYALR
                     CGVDEELIRTAARRIAGADSVAVFEDLGIQQAPNSTLCSYLNKLLWILTGNFAKRGGQ
                     HLHSSFGPLFRHTREVGRTPVSGAPIVGGLIPSNAVPQEILTDHPDRFRAMIVESSNP
                     AHSLADSAACRAAFESLELMVVIDVAMTETARLATYVLPAASQYEKCEATFFNLEFPH
                     NTFHLRHPLLEPLEGTLPEPEIWARLMRALGVIDDTELRPLREAAEQGLEAYAQAFLT
                     GVGTNPVFTKALPFVLYETLGPTLPDGLAGAAALWGLAQKAAMTYPDAVRRAGHADGN
                     ALFETILASRSGVIFTDHEYEDDWQLVTHADHKIALDMPEMLGEIRGLSDPQAELTTP
                     EFPIVLSAGERRAYTANDIFRDPSWRKRDADGALRVSIEDAHALGLTEGGRARITTAA
                     GSAEASVEISEAMLPGHASLPNGFGLDYIDPDGRATVPGVAPNSLTSTEWRDAYAGTP
                     WHKHVPARIEAVPVAAAS"
     gene            complement(22951..23352)
                     /locus_tag="VFC01_19525"
     CDS             complement(22951..23352)
                     /locus_tag="VFC01_19525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN81853.1"
                     /translation="MLKDGRVATRLPAQDLQRARAFYAEKLGLEPSEERPGGLLYQCA
                     SGVFALFKSAGESPGTFTQMGWDVDDLAATVAELRRRGVVFEKVDVPGLKTVDGIADV
                     AGNYPSKGSGEKAAWFRDSEGNMLGIGQPTP"
     gene            complement(23389..23937)
                     /locus_tag="VFC01_19530"
     CDS             complement(23389..23937)
                     /locus_tag="VFC01_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN81854.1"
                     /translation="MVAQTRTPQSTWIEAGLQALASGGPDAVRVDLLAKALGVTRGGF
                     YWHFGSREAFLDALLDAWEHRSTDEVLERVEGHGGDARDKVRMAGMLTFSKELLPIDL
                     AVRDWARRDRAVAKRLRRVDNRRMEYLRILIGTFVDDPDDAEARGMLAFSLAIGTHFI
                     AADHDGRSRRQVLERATQRLLT"
     gene            23997..24590
                     /locus_tag="VFC01_19535"
     CDS             23997..24590
                     /locus_tag="VFC01_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HZN81855.1"
                     /translation="MFGSALLDTAEHAARPWRIHEIAKDFHVLDVWALPTPGGPDGFP
                     QLVRLMATFDPAETSPVVRALFSIRWTLGRVFGLDGQDSGLGTRVASLRDRLPTDLTE
                     TPTDFDTHLFTPLYVTDDEFAMEIANQTVHGILHIGWVPDGAGGYRGQMAVLVKPNGV
                     LGVGYLAAIAPFRHLVVYPLMMRAIGRAWRQSVAGAA"
     gene            complement(24592..25182)
                     /locus_tag="VFC01_19540"
     CDS             complement(24592..25182)
                     /locus_tag="VFC01_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81856.1"
                     /translation="MRIDRERTALTVVSGALGIVAALSFSAPPAVADPAPPAPAPEPA
                     PAPAPPNPFPWLPWLAPAPPAEPAAAPADPAIPPPAPAPGPAPDGTVQAAAAPATTSD
                     GVPHLLSPENLPPGTTDTPTPQGSGSYLRDLWHAYQTQEISGRDALLLLSQRPANSAP
                     PPGMPANPTPPLPSEPAPPLPPEPAPPVLPEPAPTP"
     gene            25406..26677
                     /locus_tag="VFC01_19545"
     CDS             25406..26677
                     /locus_tag="VFC01_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN81857.1"
                     /translation="MPSPDLPPGFDFTDPDIYAERLPIEELAHMRKVAPIWWNEQPKG
                     VGCFDDDGFWVLTKHKDVKEVSLRSDIFSSNAKTALPRYREGADPESLERGKVVLLNQ
                     DAPHHTHLRKIISRAFTPRAIERLRDELNERAKNIVKAATAEGSGDFVEQVSCELPLQ
                     AIAGLMGVPQDERKKLFDWSNQMVGDQDPEFANNDPMAASIELIMYGMQMAAERAKNP
                     GEDLVTKLVQADVEGHKLTDDEFGFFVILLAVAGNETTRNSITQGMMAFTEFPDQWEL
                     FKKERPATAADEIVRWATPVTSFQRTALEDTELSGVKIKKGQRVVMFYRSANFDEDVF
                     DDPFRFNILRDPNPHVGFGGTGAHYCVGANLARMTIDLIFNAIADEMPNLTPISQPER
                     LRSGWLNGIKHWQVDYLGRSKQPVAQSSGSR"
     gene            complement(26607..26942)
                     /locus_tag="VFC01_19550"
     CDS             complement(26607..26942)
                     /locus_tag="VFC01_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81858.1"
                     /translation="MRTEQNIPRWLRFVLISDRAGSAWYIGAGFFFAPVLVVVSPWPT
                     LTAVCWAIIGLAGLWLGLLGIAMATGLAMVLRTGDEIPEDYWRSIIDYPTTARPAVST
                     GRDSRPASV"
     gene            27177..27914
                     /locus_tag="VFC01_19555"
     CDS             27177..27914
                     /locus_tag="VFC01_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN81859.1"
                     /translation="MRQWRQRRRLSQLDLAIEADVSARHVSFIETGRSAPSREMVLRL
                     AGVLDVPLREQNQLLMAAGLAPVYRERSLADPDMAAVREGVERVLNAYNPFPCVVVDR
                     GWQLVHANAGAAVLLDGVAPHLLERPNALRIAVHPEGLAPRIRNLGQWRHHLIERLRR
                     EAAVSGSAELSALLAEIESYPGGLDPTRDLGGVAVPLELYTSDGKLLTFLSTVTTFGT
                     ALDLTAAELSIEAFLPADEATASALRG"
     gene            27957..28373
                     /locus_tag="VFC01_19560"
     CDS             27957..28373
                     /locus_tag="VFC01_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HZN81860.1"
                     /translation="MQTRHIAVAAAVLVVGLAGCGGQPAALGGTTAKVTIDGKDTGNP
                     HAVRCSQTGWAWTIETAGKDKGFTAVLGTGDKVAVKSVDFRDFAGFSGTTWGDIGKAE
                     VTGVGGKYTITGSADGNFTDNPSKDVTATFRIEANC"
     gene            28374..28751
                     /locus_tag="VFC01_19565"
     CDS             28374..28751
                     /locus_tag="VFC01_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN81861.1"
                     /translation="MAHPFRRAIEARDLEAAVALLREDVVFRSPVVFKPYHGREALRL
                     ILQAVLAVFEDFHYVREIGADDARDHALMFEARVGDKRIEGCDFIRLDQDGAISEFTV
                     MVRPMSGMLALAEAMKVQLSALE"
     gene            28801..29523
                     /locus_tag="VFC01_19570"
     CDS             28801..29523
                     /locus_tag="VFC01_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HZN81862.1"
                     /translation="MALHPVNRRSVPEDVFEQIVAEVLSGDMKPGEALPSERRLAEVL
                     GVSRPAVREALKRLTAAGLVEVRQGGATTVRDFHRHAGLDLLPRLLIRDGELDLSVVR
                     SILETRLHNGPKVAELAARRRRPELSAQFDDVLQKLAAEGDPVEQQRLALTFWDHVVD
                     GADSIAFRLMYNTLRATYEPALAALATVMADEVGRPAAYRELADAIITGDPTRASKAA
                     HDLLEPATTGLLTAIDTLEEQR"
     gene            29520..30194
                     /locus_tag="VFC01_19575"
     CDS             29520..30194
                     /locus_tag="VFC01_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HZN81863.1"
                     /translation="MTTVTERPTRKGFTLADASREFWKQPSPWMIGATFVVALTARLN
                     VGDWQITDALVPIVMIALFPLVEWIIHVCILHWRPKHLGKLTIDPLLAREHRAHHTDP
                     RNVRLIFIPWKSLAFWVLPLAVAVALLAFPRLGMGLTYLVFLSALGLGYEWTHYLIHS
                     NYKPKTRVYRAIWRNHRNHHYKNEHYWFTVTSSGTADRVLGTYPDPATVATSPTAKNL
                     HATAER"
     gene            complement(30178..30933)
                     /locus_tag="VFC01_19580"
     CDS             complement(30178..30933)
                     /locus_tag="VFC01_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZN81864.1"
                     /translation="MQTVRRAVRNVLVVAGIAVIATAGSVATGSAAVTAPTDVVNIQP
                     AQGQKVGVAMPVTVTFAQSISNRAAAERTISFTSSAIPAGSFTWLNDRVVQWNPTGFW
                     PAHSNIIVTAGGAKTSFETGAATVGVADIDAHTFTVSIDGQVVRQMPASMGKSRFPTP
                     VGTFAALEKQSVVVMDSRTIGIPLSDPEGYKLTVNDAVRVTWGGIYVHSAPWSVGSQG
                     NSNVSHGCINLGPDNAAWYYDTVHIGDPIIVQQ"
     gene            complement(31029..>31602)
                     /locus_tag="VFC01_19585"
     CDS             complement(31029..>31602)
                     /locus_tag="VFC01_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HZN81865.1"
                     /translation="RVTLRVAIVLAVLLALVAVATSTRSGLLWRLSTFTYQVNVLAAA
                     YYLWTLARPRADARVGIRGAVVLYVLIAGVIWNLFLTGYSMGYTPANMLLHVVVPVLA
                     LTDWLLVGRDQAQVRWWQPVAWLAYPTAYLALALVVLNNAGRRAPYYFLDPDTVGTAT
                     VVMNICVLAGGALMLGYTLLVINRAVATVL"
BASE COUNT         5402 a        10520 c        10388 g         5292 t
ORIGIN      
        1 tcggcaagac aaacaccccc gaatggggca accactgcac caccgagccc tcgctgttcg
       61 ggcccacggt gaacccgtgg tcggcgtcga tcacgccggg cggctccagc ggcgggtcgg
      121 ccgcggcggt ggcggcgggc gtagtgccgg cggcctccgg cggtgacggc accggttcga
      181 tccgggtacc cgcgtcgtgt tgtgggcttg tcggtctcaa gccacgccgc ggccgtacct
      241 ccttcgcgcc gggcgccggc catggactgg agggactggt caacgagcac gccctcaccc
      301 gcaccgtccg cgacagcgcc gcactgctcg acgcggtggc cggctcggct accggtgacc
      361 cgtacaccgc gcccgtgccg aatgtgccat tcctgcaagc gatcacgcac cagccggccg
      421 ccacgcggat cctgatcgcg accgcgtcac cgtatcccgg accggccacc catcccgagg
      481 tcgtcgccgc cgtcgaacag accggcaccg tgctcgcccg cctcggccat gtggtcgaac
      541 ctggcgcccc gaccatcgac gccgatgccg tcgcggatgc catcgcggtg ctgcacaacg
      601 tcagcaatgc gcagttgcac gccctggcca ccgcgcacct cgggcgggag ccccatgagg
      661 acgagttcga gccgagcaca tgggtgatgg ttcgcgaggg tttcacgacg agcggggtgg
      721 catacgccga ggcgatcgcg gccgtccacg cgcagacgcg gcggttcgtc gcaggcatgg
      781 ccaaccatga cgtgctgctc gtgccgacgc tcctgactcc cccgccgccc tacgcgctgc
      841 tcgatcagcc gcgcggcacg acccgggcgt tcttcgacgt cgagttcgcc accaccggct
      901 ggacgtcgtt ggcgaatgtc actggctcgg ccgcgatctc gttgccgctc ggcgtcacct
      961 cgaacggact accgatcggc gtccagctga tggcgcccga cgagacgatc ctgctgcagc
     1021 tcgccgcaca gctcgaaacg gcgatgccgt ggttacatcg gcggcccagt atgtaatgtc
     1081 gccacatggt gcgcgatatt cgtgagctga ccgattcccc gcaggcctac gccgaggagc
     1141 tgcgccggcg ctggggcggc ctactgagct accggtacat cggccgcagc tacgcatcga
     1201 tggatctcgg acccgaagac aacaccgtga ccatccgcca cgacatgcgt aactccacgg
     1261 gcggactgct gctggccgtg ctgtgcatcg ccgcacccga gggcggccac atgtcagacc
     1321 tggaggcggt gccgaacccg gtcatccatt cgtgtcagat cctcgacccg ggccgcgatg
     1381 ttcggcgcat cgagatcgtg tccgaggacc taaagcgggg ccgtcagatg ggctacagcc
     1441 gcgcgaagat cgtcgacgcg gaccgaccca gcagggtgtt ggcactgacc caaggccagg
     1501 gcgtcagcat cggcgtgccg cccgaaggcc tcgaacggat ggaggccaac cccctcgagg
     1561 tgatcgactc cctcgacctc ccaccgctct ggcaggtctt cggcggccac cgacgaccgg
     1621 atggccactg ggcattgccg gaactcgcgg tcgaggtggc gtctcccgac gccgcattgc
     1681 acatcggacc acagttcgtc atcggagaga ccgctgcggc cgacgcggcg gctgccctcg
     1741 ccggaaccga cagcctgcag gccatctcgt cgcacgtgat gttcgttgcc cgcggcaagg
     1801 tcggtccgtt ccgcgtcgaa accgaaccga tgcccggcgc tgacggcacg ctcgcggtcc
     1861 gcaccatcac gcacgacgaa ggcgtcgagg accgcatcac caccgtgggg tcatacctgt
     1921 ttcgccgggc cgattaggcc gcgtgcgaca ctttcggcat ggctgtctca atcgcgcgtc
     1981 cgaagctgga aggcaacatc gccgtcggcg atgaccgtca gatcgggttt gccgagttcg
     2041 gcgatccgca gggccgtgcc gtgttctggc tgcacggcac acccggcgcc cgcaggcaga
     2101 tcccgatgga agcgcgcgtc tacgccgagc agcgcgagat ccggctgatt ggtgtcgacc
     2161 ggccgggcat cgggtcgtcc acgccctatc aatacgacac cgtcttcgcg ttcgccgacg
     2221 atctgcacac catcgccgac accctcggca tcgacaagtt cgaggtgatc gggctgtcag
     2281 gcggcggtcc gtacacgctc gcgtgcgctg ccgccatgcc cgaccgagtg gtcgccgccg
     2341 gtgtgctggg cggggtggct ccggtgcgcg gcgcggatgc aatcggcggc ggcgtgtcaa
     2401 cgctgatcag cgcggcggcg ccggtagtcg aattggccgg cgcgccgctg cgattggcgg
     2461 ccagcaccct gatccggctc atccggccgg tcgccgagcc ggcgctctac gcgtacgccc
     2521 gaatttcgcc ggaggccgac cgtcagctgt tggtccgccc ggagttcaag gcgatgttcc
     2581 tcgacgacct gctcaacggc agccgcaaac agctcgccgc gcccttcgcg gacgtcgtgg
     2641 tgttcgcccg cgactggggg ttccggctcg aggaggtcaa ggtgccggtg cggtggtggc
     2701 acggcgaccg cgaccacatc gtcccgttcg agcacggaca gcacgtggtg tccaagcttc
     2761 ccgacgccca gctgactcac ctgcccggcg aaagccacct ggccggacta ggtcgggccg
     2821 aggaaatctt gggcacgatg gtcgagctgt gggaccgcgc cgaccagaag tgattaacct
     2881 ccggcggcct cgatgatgag gtcgcccacc tcgcggggtt tggagacgat ggacaggtgg
     2941 ctggcgtcga gctcgatggt cttggcgttc atccgctggg ccatgaaccg ctcgaggtcg
     3001 ggattgatcg tgcggtcgtt cttcgacacc gcatagaacg tcggcttgtc gcgccaggcc
     3061 gcttgagtgg tgcggccgtt gaacaattcg tcggagatcc tgccctgcac cgtgtacaac
     3121 accctggccc gggctgcggg gacgtcctgt gcgaagtcgt tgaggaaggc cgactcggat
     3181 agctgcgcgt atccgtcggg ggctttgacc aaacctgcgc tcgctggcgg ggccgggtac
     3241 tgcgcggcca gtgcggtgta gtcctcgccg gcgtcgggtg cgcgagcggc gatgtacacc
     3301 aaggccgtca ctttggggtc gacaccgacc tcagtggcga tcattcccga atacgagtgg
     3361 caggcaagca ctgtcgggcc gtccatcaaa gccagtgtgc ggcgggcgaa ctcacagtct
     3421 tcgtccagcg tgcgcagcgg gttctgcaca gacgccacgt tcagcccgcg ggcctgcagg
     3481 tacgggatca cgtcggacca acacgagccg tcggcgtacg cgccgtgcac aagcaccacg
     3541 ttgcgggcct ggggcatggg atcgggcggc ggcgccgccg atgcgcgctg cgccccgatc
     3601 agcgcggcgg cgctgccaag agttaccgcc gctgcgaacg atcgtcgatc gatacgcatg
     3661 cgcgctcctt ccaaggggcc cggctaaggg tcccttacgc gcttgacgca cgcaatcccc
     3721 ggaatgatct cctagaagcc gtagccgagg acagccggga cgtcgctcac gaatgctgcg
     3781 tcgagccggc ccagaagggc ggagtcgttt gtgtgcaatc ggtttgccgc ggcatatgac
     3841 gacgctttgt gagcacccat atagaggctg cccagcacat cgaggtccat gcggacgtcg
     3901 gcctcggcgt ctgttggtgc gcagcgagcc cgtccgtcgc gaatcttgag cgcgaaccgc
     3961 ccaccgtcac tgcggaatcc atctgaaatc tcgatcacga tggacacatc cgcctcatac
     4021 gtacgtgcct cgagcgtcgt cggaatgtcc atgatgcgca gccagagatc gtcctcgtaa
     4081 tgagtaagac gagccacccg aggatccgcc aacaggtacg gcagcggatc ggcgggatgc
     4141 gtgccgaaga cgaccttctc catcaggtcg aggccgaaca gcgcccgaca caacgcaata
     4201 cccgcgtcgg tggtcgcggg gaccaactcg ccgactcgca cgatcatcgg gtcgtcgccg
     4261 aacacccgat aaagggcgta gccgtcggga tggaggaacg cgaacaactc cgttccaccg
     4321 cggcggaccc actcacgatc ggcgagcaac tcgtcccaca gcgccttcgg gcgaacgacg
     4381 ccgcccggtg tttggcgccg ccagcgttcg tagatctcct cgaggtcatc gcggtggtcc
     4441 gcgggcttca cgatccggac accaccggga tcgggagcgt cgtcgcggaa gcgcgcaaac
     4501 cggcggtcga cgctgaactc ctgctcgatc gtcgcaggcc catacccgaa gcgcccgtag
     4561 atgccgcctt cgctggcggt caggcccgcg atcggatacc gcgcgtcggc gatgcggttg
     4621 tgcagttcgg tgtacatcga gcgcaggact ccgcggcggc gatgagtcgg cgcgacggcg
     4681 acgatcgtca cgccggccat cggcagctcc gcgccgccgg gcacggtcag ccgcatatcg
     4741 aggtaatgcg ccatgccgac gacgttgttg ccgtcgcagg cgatcaccga gctgtcggtt
     4801 ggcatcaggg tccgccaggc agccaacgcc tccgggtgtc cgaagacgcc gaaactggtg
     4861 gagtccaaca gtgatatcgc cgcccagtcg gcgtcggcgg cggatcggat ctctacggaa
     4921 gtcgatgtgt cggaggcgag cacagaactc gacggtgcca caaagcaatg ccggagcgca
     4981 tccgaatttc gtgctcagcc gataccgttg agctggcacg gaaggagcgc tcgtgatggc
     5041 gaccgatctg acgctctacc tcgatgacca ccccggcgag ctggcccggg tcggtgacgc
     5101 gcttggcaag gcgggcacca acatcgccgg cctgtgcgcg gtgaccaccg gcggaggcgt
     5161 ggccgaggtg cacatcttgg tgcaggatgc gacatcggcg ttcgaggcgc tgcaggacgc
     5221 cggaatcaag atcgccgcgg agcaggaggt gatcgtgctc gacgtggagg atcgacctgg
     5281 cgcgctcggc gaagtggccc gcaagctcgc ggatgcgaag gtcaatctga cgacggccta
     5341 cctggcgacg aacacccggc tggtgctggc cgcggacaac ctggccgaag cgaagtcggc
     5401 gcttacctga tcagcgtctc agccacccct tgaccgtgct gagatcgcgg ctgtacgtgg
     5461 tcaggacgtc gtcgtggtag agcgtgccac cgctgatcac ctctgtgccc tgccagatca
     5521 cctgcaccct ggcccccgcc ttctggtagt agtcggtgcg gtcgacgttt ctgcggtgcc
     5581 agccgccctc ctcggcgagc tcggctagtg tctgccgctc gtcggttgct gtcatcgctg
     5641 ttcctcctgt ggtgcccgcg tcggtgcgag acgatacagc gatgatggcg ctggaggttc
     5701 agcaggccga cgtgacccgg ctcgaggtcg acgcgatcac caacgccgcc aacaccgagc
     5761 tcaagcacgg cggcggggtg gccggcgcga tctcgcgcgc cggaggaccg gcggtccagc
     5821 gggaatccac cgagaaggca ccgatcggcc tgggcgaggc ggtagagacc acggccggtg
     5881 acatgcccgc gcgctatgtc attcacgccg cgacgatgga actcggcggg ccgacgtcgg
     5941 cggacatcat cgcccaagcg acccgatcga cgttggccaa ggccgacgaa ctgggttgca
     6001 gatcgcttgc cctcgtcgcg ttcgggaccg gggtcggtgg cttcgcgctc gatgaggcgg
     6061 cacggctgat ggtcgatgcc gcgcgcggac acaaaccccg gtctctcgag cgagtggtct
     6121 tcgcagtgca tggggacgag gccgaacggg ccttccgcac tgcactggaa cagtgactga
     6181 cggtacgccc acggtcggag tcgaggagga gttccttctc atcgaccccg acacccgcca
     6241 accgcaaccg gccgcggccg cggtcgtcac gcgggccgcg ccgggcctgg gcgatttggt
     6301 cagcggcgaa ttcacccgat gtcagatcga gatcaagacg ccaccgtgca gtgaggccgg
     6361 tcggctgcga accgagctgg cgcagctacg gacggcggct gcgggggcgg cggcttcaga
     6421 aggcttgcgg ctgtgcgctt ctggcacccc tccgcttgca ggccgtgagc cgtccgccgt
     6481 cggcgaccat ccgcgatatc gcgccggtgt ggcgcagtac cggtcaatgc tcgacgattt
     6541 catggtctgc tcgctacacg tccacgtcca tctgccggac cgcgagttgg ccgtgcgtgt
     6601 cagtaaccat ctgcggccat ggctgccgct tctgatcgcg gtaagcgcga attcgccctt
     6661 ccaccaaggc aacgacaccg gatacgccga ctggcgggcg gtggtccgca gtcggtttcc
     6721 ctgcctgggt ccgccgccgt atgccgaatc cctgcgccac catgaggaac tcgcgaccgc
     6781 gattgcagaa tcgggtgcga tgctggacgc cgcgacacca ttctgggaca tccggccgaa
     6841 tccacgcctt ccgactctgg aggtccgcac gatggacgtc gtcgccgaca tcgacgacac
     6901 cgtcgccctc gcaatggtga tccgcgcgtt ggtggcgacg gcgaccgaca aggcgctagc
     6961 cggcgatccc ggcccgcagc ccagcagtga ggttcttcgc gcggcgtatt ggcgggcggc
     7021 cagagacggg tggcccggct gcggcgtcga tgcgttgact caagagatgt tgcccgcacc
     7081 ggtgcaaact gcgcggctcc tcgaccacgt cgccccggca ttgcgccgcc acggcgacag
     7141 cgacatcgtg caccggttcg tcgaccggct gacggagcgc ggcacaggtg ccgacctgca
     7201 gcgcgcctcg ctggcccggc acggctcgtt aaccggcgtg gtcgacgacc ttgtcacgct
     7261 gacggcgcga acgtaatcac caggacatcg cgtcgcgccg gctcggcggg gtcgcacggc
     7321 ctgatcggcg agacgccatg cagggtccga cggtcgtcgc cgagaagcag ggtgcccggc
     7381 tcgtccagcg tggtcgtaag catgtgacgg ccgtcgatgt cggtgacggt gctcttgccg
     7441 ccgatggcgt tgcgcctgcc gatcaacagc gaggtgacga gcgtgacgcc gtcgcggtgc
     7501 ataccctccg gcgtcggcat gccgtcgtcg tcggcggacg cgaggacacg gaatggggtc
     7561 accttcgcgc tccacgtggg tgtgtcgtcg agcgccatgg ccagctgacc gagcagcttc
     7621 aggatccgtt ctagcagtgg atctttcgcg aacgcatcgg tcaacggctc gaactgccgg
     7681 tccttgccga cataaagcgg gtttgaattc tccggctgga cgaaggcatc gtgtggcatc
     7741 ggctccgcga tggcctcagc gggcgtgaat cggaaatgcc catagcggcg caaccggcgc
     7801 gtgcccagtt cggcggcata gggatcgggc accaggtcct cccagtgcgc ggcgaagcgc
     7861 gtccactcgg acgcgtcgat gtcgaggcac gccttgagtt cggccggctc gatcagaccg
     7921 gtgccgaccc gtgttagctg ctgcgcagcg gcctctggcg tcatggcgcc agaggcatac
     7981 ccaaaaacct gtcagaccca ccgatgtccg tcgcttctac ccatgaacgc ggcccaaccg
     8041 ggaccgcaac caccaaaaaa gggagcccat catgaagatc accaagaaca agatcgtcgc
     8101 ggcggcacta ggcgccgcgg ccgcacctgc gttgctgttt gcgggcgccg gaaccgctca
     8161 agcggccacc gcctgggcgt ggcccgatag cgacccgctc ggggtcacga tgcatatcca
     8221 gtcaaccggt gcctggggct ggtgcgaata caccgccgtg cccgtcggcg gcggcatcaa
     8281 gccgttgccg gcgtacaaag tcccgttcta tctgaacaac ggccaggaaa gcaccctatg
     8341 gttcccgggc atcccgacgg gcacgacgtg gaacgtgacg atcacgtgcg aacatggcgg
     8401 gctgaacaac tcgcaggttc tctactgagc ccgccaaccg gtcagtgcgc gtcgcaacat
     8461 atgcatcgcg accgtcgtcg accgttcgcg aatgtcggac cgatcgccgg gcaaccggag
     8521 cgtgcgggtg aacgtctcgc cgtcggcgag tttcacggtg aaacagaccg tgccgaccgg
     8581 cttttcatcc gtaccgcctc cgggcccggc gattccggtg atcgcgatgg cggtgtcggc
     8641 gccgaagcgg cccagcgcgc cgtcggccat cgcctcggcg accggctccg acaccgcccc
     8701 gtgctccgcg atgagatcgg cagcgacgcc gagcaggtcg gtcttcgcct cgttggcgta
     8761 cgagacgaca ccgccggcga catacgccga cgaacccgcc aggtcgctca gccgcgcggc
     8821 caacagtccg ccggtgcagg attcggccgt tgcgatccgg cggccggcga gcagctgcgc
     8881 gatctggtcg tcgacacgcg tgccgtcctc ggagaagatc tcgcggccgt ggcgctggcg
     8941 cagcagttct aagagtttcg cgtagatgcc cgcggcgtcg ggttcgtagc gggtgacgat
     9001 ctcgagctcg ccgcgccgca ggcaggtggt gatctccagc cggtcgaaac cctcgatgct
     9061 ctgttcgccg tctcgcaatg tgtcggccag accggactcg ggcagcccga acatccgcac
     9121 catctcctgg cgatacgtgg tgcgcccggc gatcgctcgc tgcaccgcct cggtttccac
     9181 ggcgaccggc cacatcggct gcagctcgcg cggcgggccg ggcagcacca cgatcgtcgg
     9241 cttgcccggc acgacgactc ccggagccgt gccgaccgga ttgatggcat gggcgccgac
     9301 gggaaccatc gcctgcttgc ggttggcggc ccggaccgcg tcgatgtcga cgttgtcgaa
     9361 gcgcgccatc aatcgcttca ggatgtcggt gatgcggtcc tcgagggccg catccagcac
     9421 cagttcgagg ccggcgaatt tggccaccgt cgccactgtc atgtcgtcgg ccgtcgggcc
     9481 gagaccgccg ctggtgacga tgatgtcgac gccctcatcg gcgaggaacc gcagctgcgc
     9541 ctcgatatcg tcgggacggt cgccgcagat agtgatgtgg gccagttcga cacccagctc
     9601 cagcagccgg tcggcgatcc aggggccgtt gcggtcctgg acccgtcccg tcagcacctc
     9661 ggtgcccgtc acaacgatgc cggcgcgtgc gctcacgtcc aacgacacta cggtagcccg
     9721 gtaacctcat tctccatgac ggcgccgaag tccctccgcg agctgttcga tcagctgggc
     9781 ctgcaggagg ttccgtcggc cgacggcacc gtgaccatgg agatgcccgt cgacgaacga
     9841 acggtcaata ccgcaggcgg tcttcagggc ggcctcgtcg cgaccatggc cgacgtgacg
     9901 gccgggcaac tggccgcgcg cagcacccca ttcggtatgc gcatcgccac caccgacttg
     9961 ttcatccggt acctgcgacc catcaaggtc gggccggcac gggcggtcgc gcgaatcttg
    10021 cgcaccggca agcgatccgt cgtggtgcaa gtggacatct accgggccaa cgacgacgag
    10081 gtcggcgcga ccgcgacggt caacttcgcg gcggtggact gaccggcacc acgacgggcg
    10141 gcgtgttgta acccgtcact ctcacccagt tggcggtcgc accggtccag ggctgtccct
    10201 gcaattcgac cgtctcgccc gggaacaccg tcacgtagtt gtcgctgtat tcgacgggca
    10261 gaatttcgtc gtcctccggc gtggagacga gttcggcgcg ttcgaagaac gcgacccgcg
    10321 acgacgagtt gcgcaagcgg atggtcacgc gattgtcacc ggagttggcc gctctggatg
    10381 cggtcacatc cagcggcacc tgcggcatgt agttgagccc ggtcatgtcg gcccaactgg
    10441 tctgcgtgag ttcgaacgcg gaatcgttgc gcggatcacc gacgtcgtcg ggccgctgcg
    10501 actgccaata gacgttctcg gacactactt ttcccgccca gtcgagaagt tgggcgcgca
    10561 cgaagaagac actcgaatca cgggcgagtc gcggcaacgt caacgcctgt gtcgttcccc
    10621 ctggcgtcac gtagatgttg cccgcgctgc gatcgtcacg cactcggccc tgcaggtcgt
    10681 acacccggac acgaaccgtc aaacccgttt gttctcttgg tgtttggttg acaacggtga
    10741 tgttggcctg gtcatggttt cccgtcgcgt acgagtcgaa gacgaccgac aacggccgca
    10801 ggcccttctt cgcaccgaaa tacgcaccgc cgggccgcag gtagtagtcg aagatgtgtc
    10861 cgaagaacga cggccagtgg ttgttgagca tccagtagat cgtcatcttg tgcgtgtccc
    10921 agccgccggc tgcgaacgcc tcgaactggg cgcgggtcga ctcgtaatgc gccaactgcg
    10981 ccttgcgcgc gaactcctcc gcgccggtgg acaccccgta ccgacggtcg accgcacgcc
    11041 gaatgctggt cagcgcagcg tttttcgagt tcgagccggc gtggaagaac catgcgtcgt
    11101 tgatcggcca cagcttgtcg ggcgggatga acttcttcag gctcgcgaat ggcgggatct
    11161 gttcgttgtc gccttgttcg gctgtcgacc cgcgggtggc ggcgtatcgc ccggcgaacc
    11221 agtagctcgg cggtcgccag ctgtacgggc ccgccatctg aatgccgtcc cagtcgcgct
    11281 cccccgccgc gtcggtcgcc aacgaggaga cggtgtcgac gatcgcgttc ggccagtgca
    11341 gatccgacag gatggcgtgg tacttggcca acacctcggc tgggggtttc ccgtcgctgc
    11401 cgttcgccca gatgaacacc gacgggtggc tgcgaagccc ctcgatctgc gaacgcaggc
    11461 tgtcttgtgc gacgcggttg tcctcgccgt cccactgatt ccatttctcc cactggttgc
    11521 agcacatcca gccgtacatc agtggaatgc ccatctcgtc ggccatctcg gcgatgtgat
    11581 cgccggggaa tttgccttcg aggcgaatca tgttgagccc caagtcttta acataaccca
    11641 gaatcgcggc gtcgcggctc ggatcgttcg cgtacagcag gtccggcgta tacgccgcgc
    11701 cgcgcaccag gaagtccttg ccgttgacct tcagatagaa attgccgccc ttgcccagtt
    11761 cgaggaattg ctcgtcttgg tcgcggaatt gctgaacgga tctgatgccg aagcgctgat
    11821 ggctggtgcc gatcggtcgg ttgtactgcc ggaattcgag ctgcaggtcg tagaggtcgg
    11881 gccggccgag ggtgtagggc caccacaggt ccgggttgcg gacggtcaac tgcgcgaacg
    11941 tgtcgggggt aaagctgatt tcacgctgct cgcctgccgc gagcgtgacc ggctgctcga
    12001 tctggacgtc gggcttgccc gggcgggtga tcgtcgcccg cagaacgccg cgcagctgtt
    12061 gtgcggacgt attccgcagg ttgctgtata tcgtcagctt cgccgaattc gttcgtggca
    12121 gcggaagttc ggaggtgacc gtcgatgagc cgagcaccac agcgccggac actttgagat
    12181 agaccggctt ccagattccc gcattgcggt cgggcacaaa ggaattcccg ttcgccgggt
    12241 tcttccctgg cccctggtaa ccgagatagt tccagttgat ccagtcgtac cagttgtccg
    12301 ccagttcgac gccgtcgata tcctgcagcg cgcgctccgg ggtgatcttg accgctagcg
    12361 cgttcgaccc cccctggttc acccaccgcg agacatccag ttcgtgggcg gtgtgcatgc
    12421 cgacgatctg cgtgttgccc gcaatcaggt ggccgttcaa ccagatctcg gcgcgatagt
    12481 tgatgcccgg aaactccaac aggtacgtcg tgtggccgga cggcgcggtg aacgtggtgc
    12541 ggtaccacca gtcctgcttg tagaggtcct gcgggacggc ccccagattg gtgccgtagt
    12601 agaggtccgg gtaggtgccg tcttcttgca gggtctgcag gaccgtcgcg ggcattcgcg
    12661 ggatcgcatg ccagccggct gcctggtagt cgggcttgga gaggacggcg ccgtctgccg
    12721 gcacgttgcg ggcggaaacc aggctccagc cctgcgccaa ctcgatctgc cccggcgacg
    12781 gccctggttt ggcgaagtcc gggaagtcag ccgcgcagag gagcagtaac agcgccacgg
    12841 acagcaggat tcttaacacg atgtgccgac gccggtaacc cgacatctgc accccgtcca
    12901 ccggaacacc ctacctgcag cgtgaggacc gggcccggca agcagcgcaa cggaattcgc
    12961 gctggtcact ccgtgcgcgc caacacctcc cgcaccagct ccagtgtggt gtcaggatgc
    13021 aggaacgcga accggcccac tgtttcgccc tcccatttcg tcggcgggat gaacgcgacc
    13081 tggtcctcgt gtagccgctg ggcccaggcg tcgtagtcct cgggcttcca gcccagccga
    13141 cggaacaaca ccacgccgag gtccggctcg cggatcagct ccagctgcgg tgccgacttg
    13201 atcagctcgg ctgtctgcac ggcgagcgac gccgcgacct caatggcccc gcgatacgcc
    13261 tccagcccgt acaccgccat cgagaaccac aacggcaatc cgcgggcccg ccgggtcagg
    13321 tgataggcat agtccgacgg gttccactcg ccatcggacg tgtggatgac gtcgaggtac
    13381 gacgcatcct gggtatgggt gccgcgcgca agctcggggt cgcgatacag caatgcgcag
    13441 cagtcgaacg gcgtgaacaa ccacttatgc gggtcgagga tgaacgagtc cgcttgttcg
    13501 aggccgttgt acttcggccg cagcgagcgg gcgaagatgc ccgagccgcc gtaggcgccg
    13561 tcgacgtgga accaccagcc gcgttccttg gcgagcgcgc cgaccccggc gaggtcgtcg
    13621 atgatgccgg cgttcgtcgt gccggatgtg cagaccaccg cggcgatgtt ggcggcatcg
    13681 ggcggcacgg cctcccgaac ggtgtcggcg gtgagccgat ggtcaggtgt atcgaccacc
    13741 agtgcgtcca tctcgagcag ccgcagcgtg ttgacgatcg acgaatgcgc gtccgagccg
    13801 acgacgacgc gcagccggcc ggtggcgccg cgtttcttgg ccgtctcgcg agccacagcc
    13861 agcgcggaca agttgcccgc tgaaccgccc gagacgaagc agccgcctga cgtcgtcggg
    13921 agacccgcct cattggcgat cacccgcagc acgctgttct ccgccgcgat cgcgccggac
    13981 gcctccagcc acgagatgcc ctgtattgag gcgcaggaca ccagcatgtc gaacagcagg
    14041 gcggccttgg tcggggcggc cggaatgaag ccgaggaacc gcgggctgtc ggcggagatc
    14101 acgctcggtg cgatcaccga ggtgtagacg ccgagcacct cgtcgggctg gcgcggctgc
    14161 tcgcggatca ccccgtcgag ccggtcgtga agctcatcgg cgggcagcac gcccttgtcg
    14221 agcggtacag gattcatccg cagccggttc tcggcgtacg cgagcacact gcgcaccatc
    14281 tgttcggtag tttggtcgac ctcgtgcatg gctgaagtct cgcaccggcg gcatgcgcgc
    14341 cgatgcgtta cgcgcgagtc agattcggtg cggcacgtcg ttgaccaagc cgccgtccat
    14401 gacgaactcg gcgccggtgg cataccgcga ttcgtcgctg gcgaggaaga cgacgaacgt
    14461 cgcgacctca tcggactgcc ctggtcgacc gagcgggatg gtgagcatgt tgtcggggaa
    14521 gtgcttggtc atcggggtac gaatgaagcc cggatggatg gagttcaccc ggatctgatg
    14581 cgacccgagt tccagcgccg ccgatttggt caggccgcgc accgcccact tcgatgcgac
    14641 atagggatgc accatgacgg cgccacgcag tccctcgata gacgagacgt tgatgatcga
    14701 accgccgccg gcttccttca tcgcctcgac cgacgcctgc atgccgagga acgtgccggt
    14761 caggttgacg tcgatgacct tctgccactt cgccatgtcg aacttgccga tctggccgag
    14821 cgcgacgatg cccgcgttgt tgaccaggac gttgagcttg ccgaactcgt taaccgcggt
    14881 ggccacggcg gcgtcccact cgtcggcctc ggtgacgtcg aggtggacgt agcgtgcggc
    14941 ctcaccgatc tcgtccgcga gcgccttgcc ttcgtcatcg aggatgtcgc cgatcaccac
    15001 cttggcgccc tcggccacca gcgcccgcgc atgctcggca cccatgcccc tggcgccgcc
    15061 actgatcagt gcaacttttc cgtccacgcg tcccatgacg ggtcaggcta ccttgccgac
    15121 gtgctacaag tagaacctgt tccagttctc taggagacat catggcgatc cgcgtcgcgc
    15181 tcatcggcac cggcaactgc ggcagcctcg cgctcaagca actggtcaac gatgcgcgct
    15241 tcgacctgac cggcgtctgg gtgtcgtcgg acgccaaggt gggcaaggac gcgggcgagc
    15301 tggccgggct tggcgtgacg accggagtca ccgcgaccga cgacctcgac gcgatcatcg
    15361 cggtcaaacc cgactgcatc gtgtactgcg cgatgggcga cacccggctg ccggacgcca
    15421 tgaccgaagt tcgcaagatt ctcgccgcgg gtatcgacgt cgtcgggtcc gggctgggtg
    15481 tgttgcagta cccgtggcag gtgattcccg acaagtacat ccatcgcgtc gaagacgctg
    15541 cgcgagaagg caattcgacg gtcttcatga ccggagtcga cccggggttc gcgacggatc
    15601 tgctgccttt cgcgctggct ggcacatgcc agcgcatcga gcaggtgcgc accatggaga
    15661 tcgccgacta cgcgacctac gacggggcga cggtgatgtt cgacgtgatg ggcttcggca
    15721 acgagatcgg cgacctcccg atgctttatc agccgggcgt gctcagcatc gcctggggta
    15781 ccgcgatccg gcagctggcg gcgggtctcg ggatcgaggt cgacgagatc aaggactgcg
    15841 tcgaacagga gcctgcgccg gaggacttcg aggtcgcggt cggcaccatc aagaagggca
    15901 ctgtcgccgc ggtgcgtttc cagatcgagg gcatggtcaa cggcaaacca gtgatcgtcg
    15961 tcgagcacgt cacccggctg cgcggcgatc tgcgaccgga ttgggcgcag cccgctcagc
    16021 ccggcgggtc gtaccgcgtc gagatcaccg gcgagccgtc ctacaccatg gacgtctgcc
    16081 cgacaagccg caacggcgat cacaactacg cggccatcct cgcggcggcg ggacggatcg
    16141 tgaacgccat ccccgacgtt gttgcggcgc ggcccggcat ccgcaccacg ctggacatgc
    16201 cgctcgtcac gggtaggggt gtgtacgccg gctagctaag ctcgggctac atggacccgc
    16261 acgcgtcgcc ggacctggtc gccgagatcg gtgccggccc agaggggcct ggagtcggcg
    16321 cgttcttcga cctcgacggc accctggtag acggcttcac cgccacggca cacgccagtg
    16381 atcgcatccg gcgcaggcaa gcgcgcatcg gtgaggtgtt gggcgtcatc gaggcgtcga
    16441 tgcgataccg attcggccgg atgcagtttg agaggctgct gacgagggcc gcgggttatc
    16501 tgcgcgggga atcgctcgcc gcactcgatg tggtcggaga acggctgttc gtcgagcgcg
    16561 tctcctcacg cgtcttcccg tacatgcacg aggtggtgcg ggcgcaccag cagcggggcc
    16621 acaccgtggt gttgagttcg tcggcgctga ccattcacgc cgaacctgtc gcccgctttc
    16681 tggagatcaa ccacgtgctg tgcaaccatt tcgaactcga cgagcacggc ctgctcaccg
    16741 gccggatcgc caagcccgtc atctggggaa cgcgcaaagc cgccgcggtg caacagttct
    16801 gcgacgccaa cggcgttgat ctgcaacgta gttacttcta cgccgatggt aacgaggacg
    16861 cggcgctgat gaagctcgtc ggccagcccc gcccggtgaa cccgcgaccc aggctggccg
    16921 cgattgccgc agaaaacggc tggccggtgc tgcaggtgac tgggtccagc ggccgcaggc
    16981 gtctcacgtt gcgcaggctg gtcggctacg actgagtcgc gcccctaatc cccgtaccgc
    17041 gaaccggcgc aaatccctaa gcctgcgacg gcgaatcgcg gtgcgcgccc cattcccgca
    17101 cgctgcctgc tgtttccaga atgactttca ccaggcggtc cttcgggggg tcgccagcac
    17161 cttaggagac gacatcatgc aactttcccg tcttggcgca gtcactgccg ccgctgccat
    17221 cgcattcggc gcgctgctct ctgcgaccgc acccatcgcc aacgcggaga agatctcgga
    17281 gacgaccatc aagagcgaat gcaagtcggc cggtgggacc tatacctcgg tggtcaaagg
    17341 tggcacgcgg ttctcgacgt gtgactacaa ggacaacgag ggcaacggct cccgcgacta
    17401 ctacgcgaac ggcgagtact actccacgag gcccacatag ggctgcagcc cggcatagcc
    17461 ggcgagctgg ctccgttgca cgaagaatgt tcggtgtcat gctctatccg gatcagccga
    17521 tcggagtgct gatcgagcgg atcgcgtggc tggagagcct cgggttcgat caggtgtttc
    17581 ttcccgacca cagcggcaat ctgaggaatc ggcgcggggt gtggttcgac agctgggcgg
    17641 tgctcgccgc cgcggcggtg acgacctcac gcgtcagact cggcgttatg gtcgccaacc
    17701 agatcctgcg cccgcccgcg ctgctggcga agcaggccgt cactctcgac catatggcca
    17761 acgggcggtt tgagctcggt atcggcgcag gcattttcgc gtgggaccat ctcagtgtcg
    17821 gcgaacagcc gtggccccca agggaacgcg cgctccgatt caaggactat gccgccatcg
    17881 tcgacggcct cctccatggc agcggtgcgc cattctccta cgccggtgaa cgtctgtggg
    17941 tggacgacgt gatacccgca ccaacaccgc tgcagtcccc ccggctgccg atcatcgtcg
    18001 gcggtcaacc ccccaccgtg atccgtgtcg ccgccgagca cgccgacgtg tggaacaccc
    18061 acggacgtcc cggcgccgcc gccgacgagg tgctcaccgc caccgcagaa caatcccgca
    18121 ggctcgacga gcttgccctc gccgccgggc gcgaccccgc atcgatccgg cggtcgtaca
    18181 caatcttcgg cgagtgggat ccccgggccg gccgctacac atacgaggac gtgttcgaca
    18241 gtttcggtgc aatcgggatt agcgatttcg tgctcgactg gccggacgat gactatgtgg
    18301 acaccttcga agccgcagca cgcgaggtac ttcccgcgag gcggcgaggc gcctaagcta
    18361 aactagaaca cgtttcagtt cgccgaccgc cccaccggag gagcaggcat gcacaccccc
    18421 atctgcgacg agctcaacat cgagttcccg atcttcgcgt tcacccactg ccgtgacgtc
    18481 gtcgtcgccg tcagcaaggc gggcggcttc ggagtcctcg gcgcggtcgg cttcacccct
    18541 gagcagctcg agatcgaact gaactggatc gacgagaaca tcggcgacca tccctacggc
    18601 gtcgacatcg tcatcccgaa caagtacgag ggcatggatg ccaccgacct gacgcccgag
    18661 gtgctcaaga agcagctgaa cgcgcttgtc ccccaggagc acatcgactt cgccaagaag
    18721 attcttgccg atcacggagt gccggtggac cacagcgacg acgatgcgct gcagctgctt
    18781 ggctggaccg aagcgaccgc gacgccgcag gtcgaggtgg cgctgaaaca cccgaagtgc
    18841 acgctgatcg cgaacgcgct cggcaccccg cccgccgaca tgatcaagca catccacgac
    18901 gagggccgca aggtcgccgc gctgtgcgga tcgccgtcgc aggcgcgcaa gcatgccgac
    18961 gcaggcgtcg acctcatcat cgcccagggc ggcgaagcag gcggacactg cggcgaggtc
    19021 ggctcgatcg tgttgtggcc gcaggtcgtc aaggaggtcg cgcccgtccc ggtgctggcc
    19081 gccggaggaa tcggcagcgg ccagcagatc gccgccgcgc tggccctcgg tgcgcagggc
    19141 gcgtggaccg gatcgcagtg ggtgatggtc gaggagtccg agcacaccga ggtccaacac
    19201 gccgcgtatg ccaaagccac cagtcgcgac accgtgcgca gccggtcgtt caccggcaag
    19261 ccggcgcgga tgctgcgcaa cgactggacc gaagcctggg agaacccgga caacccgaag
    19321 ccgctcggca tgccgctgca atacatggtg tccggcatgg cggtcgccgc cacgcacaaa
    19381 tacccccacg agagcgtcga cgtggcgttc aatccgatcg gacaggtcgt gggtcagttc
    19441 accaaggtgg aaaagtcctc agcggtgatc gagcgctggg tccaggagta cctcgaggcg
    19501 accaacaccc tcaacgagct gaacgaggcg gctaccgcct gattgcggcg gcctgcgccg
    19561 gctttctgat ggccgtgctg tggatggacc tgatcttcga cgctcaggtc cttcgacatc
    19621 gcggcgcgca cgagttgccg gaatcggtgc tcgactcgat ctcggcttat taccaccgcg
    19681 cgacgaccac gtcacggccg atgagctacc tcatcgcgct cgtcatgcta accctgttgg
    19741 gctcgttggg ttttcgatgg atcctcggtg acgacgcgac ctgggtgctg accgcttcta
    19801 ccgtgctcgc cgccgtgccg atcctgctgg cgttcgtccg caccgtgccc aacgcggtcc
    19861 ggctcggtgc ccgtgccggg agcccggcag aactgtcgca ccttgcgcgg tcgatctgcc
    19921 gagatcactt catatgtctg acgttcatgt cggcgtttct tgtgctgtgg gcggtgaccg
    19981 cttcgccgac ttgagccgga caacgttcgg gcccgggctg gtgtgccctt cggcgattat
    20041 gtaaggtgac atacatagcc gggtcggacg aagggatcca acggtcatgc ccagcccccg
    20101 cgagcggatg gtaatctctg ccgcgcttct gatcagggaa cgcggcgcgc agccgacggc
    20161 gatcgccgac gtgctcgaac acagcggcgc cccgcgcggc tcggcctacc actactttcc
    20221 tggtggccgc actcaattgc tctgtgaggc aatcgattac tccggcgagt atgtggcggc
    20281 caagatcgcc aaggcggaca gcggtctcga cgctctcgag gggcttgtcg cgtattaccg
    20341 caagcagctg cttcgctccg actaccgcgc cgggtgtccc gtcgtcgcgg tgacggtgga
    20401 ggccggcgac cccgctaagc aggaccaggc gacgccggtg atcgaccgtg cagcggcagc
    20461 cttcacccgc tggaccgacc tgatcgcgca gcggctggtg gcggacggcg tatccgcgga
    20521 ccgtgccgac gaactcgcga tgctgtctac gaccgcgatc gagggcgcga tcatcgtcgc
    20581 ccgcgcgtcg cgcgacctca agccgctcga cctcgtccac ggccagctgc gcgacatggt
    20641 ggcggccgag atgtcggaaa ggaaaccccg cgatgactag cagggatgtc gccgccgaag
    20701 cggctccgca agattggcag cagaccgcgt gcatcttgtg cgaatgcaac tgcggcatcg
    20761 tcgtgcaagt cgacggccgt aacctcgcca agatccgcgg cgacaaatcc caccccgcgt
    20821 cgcagggcta cacctgcaac aaggcgttgc ggctggacca ctaccagaac aacggcaacc
    20881 ggctgacctc ccccatgcgc aaacgccccg acggcagcta ccaggaaatc gactgggaca
    20941 cagcgatttc cgagatcgcc gcgggcttca agcgcatcgc cgacacccac ggcggcgata
    21001 agatcttcta ctacggcggc ggtgggcagg gcaaccatct cggtggcgcc tacagcggcg
    21061 ctttcctcaa ggtgctggga tcgcactacc ggtcgaacgc gctcgcacag gagaagactg
    21121 gcgaagcgtg ggtcgacgct cacctctacg gcgggcacac gcgcggcgag ttcgagcatg
    21181 ccgaggtgtc ggtgttcatc ggaaagaacc cgtggatgtc gcagagcttc ccgcgcgccc
    21241 ggaccgtcct caacgaaatc gccaaggatc ccggccgctc gatgatcgtc atcgacccgg
    21301 tgatcaccga caccgcgaag atggccgaca tccaccttcg ggtgcggccg ggaaccgatg
    21361 cgtggtgcct ggcggcgctc gccgccgtgc tggcgcagga aaacctgtgc aacgaagcgt
    21421 ttctcgccga acacgtcaac ggtgtcgaac cggtgcgcga cgcgttgcgg gacgtcccca
    21481 tcggcgaata tgcactgcgc tgcggcgtcg acgaagaact gatccgcacc gcggcacggc
    21541 gcatcgccgg tgccgacagt gtcgcggtat tcgaggacct gggcatccag caggcgccca
    21601 acagcacctt gtgctcctat ctgaacaagc tgctgtggat cctgaccggc aacttcgcca
    21661 aacgcggtgg gcaacacctg cattcatcgt tcgggccgct cttccgtcat acacgcgagg
    21721 tcggccggac cccggtgtcc ggagcgccga tcgtcggcgg gctgataccg agcaatgcgg
    21781 tgcctcaaga gatcctgacc gaccatcccg atcggttccg cgcgatgatc gtggaaagca
    21841 gcaatcccgc ccactccctt gccgattccg cggcgtgccg ggcggcgttc gagtcactcg
    21901 agctgatggt cgtcatcgac gtcgcgatga cggagacggc caggctggcc acctatgtgt
    21961 tgcctgcggc gagtcagtac gagaagtgcg aggcgacgtt cttcaatctc gagttcccgc
    22021 acaacacatt tcatcttcgg catccgttgc tcgagccgct cgagggcacg ctgcccgaac
    22081 ccgagatctg ggcgcggctg atgcgtgcgc taggcgtgat cgacgacacc gagctgcggc
    22141 cgctgcgtga ggccgccgag caaggactgg aagcgtatgc gcaagccttt ctgaccgggg
    22201 tcggtacaaa tccggtgttc accaaggcgc tgccgttcgt gttgtacgaa acgctgggcc
    22261 cgacactgcc cgacgggctc gcgggtgcgg cggcgctgtg gggtctggcg cagaaggctg
    22321 ctatgacgta tcccgatgcc gtgcgccgtg cgggccacgc cgacggcaac gccctgttcg
    22381 agacgattct cgcgagccgc tccggcgtga tattcaccga ccacgagtac gaggacgact
    22441 ggcagctggt cacccacgcc gaccacaaga tcgccctgga tatgccggag atgctcggag
    22501 agattcgcgg gctgtccgat ccgcaggccg agctgaccac gcccgagttc cccatcgtgc
    22561 tgtcggccgg cgaacggcgc gcctacaccg ccaacgacat cttccgtgac ccgtcgtggc
    22621 gcaaacgcga cgccgacggg gcgttgcggg tcagcatcga ggacgcgcac gcgctgggac
    22681 tgaccgaggg cggccgtgcg cggatcacca cggcagcagg cagcgccgag gcgagcgtgg
    22741 agatcagcga ggcgatgctg cccgggcatg cgtcgttgcc gaacgggttc ggcctcgact
    22801 acatcgaccc cgacggccgg gcgacagtcc ctggcgtggc gcccaattcg ctcacctcaa
    22861 ccgaatggcg cgacgcctac gcaggcacac catggcacaa acacgtgcca gcccgcatcg
    22921 aggccgtgcc ggtggcggcg gccagctgat tcacggagtc ggttggccga tgccgagcat
    22981 gttgccttcg ctgtcgcgaa accacgcggc cttctcgccg gagcccttgc tgggatagtt
    23041 gcctgccacg tccgcgatac cgtcgacggt cttcaggcct ggtacgtcga ctttctcgaa
    23101 aacgactccg cgcctgcgca actcggcgac cgtggccgcc agatcgtcga cgtcccagcc
    23161 catttgcgtg aacgtccccg gcgactcgcc cgcggatttg aacaacgcga atacaccgct
    23221 cgcacactgg tagagcaggc cgccgggccg ctcctcgctc ggttcaagcc ctagcttctc
    23281 ggcgtagaac gcccgtgccc gctgcagatc ctgggcgggc agccgggtgg cgacgcgacc
    23341 gtctttgagc atcgtcggtc ctttcggtcg tcgatggccc gatcgtaact aggtgagaag
    23401 ccgctgtgtg gcccgttcca atacctgtcg tcggctgcgc ccgtcgtggt cggcggcgat
    23461 gaagtgggtt ccgatggcca gcgagaaggc gagcatgccg cgtgcctccg cgtcgtcggg
    23521 atcgtcgacg aaggtgccga tgaggatgcg caggtactcc atgcggcggt tgtcgacgcg
    23581 tcgtaggcgc ttggcaaccg cccggtcacg ccgcgcccag tcgcgcacgg ccagatcgat
    23641 cggaagaagt tccttcgaga aggtgagcat gcctgccatg cggaccttgt cgcgcgcgtc
    23701 cccgccgtgg ccttccacgc gctcgaggac ctcgtcggtg cttctgtgct cccacgcgtc
    23761 cagcagcgcg tcgagaaacg cctcacggct gccgaaatgc caatagaaac caccacgggt
    23821 gacaccgagc gccttcgcga gcaggtccac gcgcaccgcg tcggggccgc cggaggcgag
    23881 cgcctgcagc ccggcttcga tccacgtgct ctgcggtgtg cgggtctggg cgaccatcac
    23941 gtctccctct cgatatgggg cttgacgccg attcttcgcc aagcatacac ttttgtatgt
    24001 ttggttctgc gttgcttgac actgccgagc acgccgcacg tccgtggcga attcacgaga
    24061 tcgccaagga cttccatgtt ctcgacgtct gggcgttgcc gacgcccggc ggccccgacg
    24121 gcttcccgca actggtccgg ttgatggcaa cgttcgatcc tgctgagaca tcgccggtgg
    24181 tgcgggcgct gttctcgatc cggtggacgc tgggccgcgt gttcggtctc gacggccagg
    24241 actccggtct tggcacgcgg gtagctagct tgcgtgatcg gctgccgacc gatttgaccg
    24301 aaaccccaac agatttcgac acgcatctat tcacgcccct gtacgtcaca gatgacgagt
    24361 tcgcgatgga gatcgcgaac cagaccgtgc acggcatcct gcacatcggc tgggtgccag
    24421 acggagcggg cggctatcgc ggtcagatgg ccgtgctcgt gaagcccaac ggcgtgctgg
    24481 gggtcggcta cctagctgcg atcgccccat tccgtcacct tgtggtgtat ccgctgatga
    24541 tgcgcgccat cggacgcgcg tggcggcagt cggttgcagg agcagcctga actagggcgt
    24601 cggcgcgggc tcgggtagca ccggcggcgc gggctccggt ggcagcggcg gcgcgggctc
    24661 cgatggcagc ggcggtgtcg ggttcgcggg catgcccggc ggcggggccg aattcgccgg
    24721 tcgctggctg agtaggagca aggcgtcacg cccgctgatt tcctgggttt ggtacgcatg
    24781 ccaaaggtcg cgaaggtacg acccgctgcc ctgcggagta ggagtgtcgg tggtgcccgg
    24841 cggcagattc tcggggctca acaggtgcgg cacgccgtcg gacgtcgttg ccggagcggc
    24901 ggcggcctgc acagttccgt ccggcgcagg tcccggcgca ggggcgggag gcggaatggc
    24961 ggggtcggcc ggcgctgcgg cgggctcagc aggtggcgcc ggggcaagcc aaggaagcca
    25021 cggaaacgga ttgggaggcg cgggcgcggg cgccggctca ggtgcgggcg cgggaggggc
    25081 ggggtcggcc actgccggcg gcgcactgaa actcaacgcc gcaacgatgc ctagcgcccc
    25141 cgacaccacg gtcagcgccg tcctctcacg atcgatcctc accgatgccc ccttcccctt
    25201 tcctgccgtg agccgtgcta ctcggtgatt cagcctcggc cgcaaaattg ttacatctgt
    25261 ggttgatgtg gcgaagcagt tattagaatt cggcattaaa gaacacgttt cacttggagt
    25321 ttgatgtaac ccgccgcacc accccccatc tgtggtgtag caatgcgaag ggggcactag
    25381 acaacgtcat gtgaggagcg catctatgcc gtcgccggac ctcccacccg ggttcgattt
    25441 cacggatccc gatatctacg ccgaacggct gccaatcgag gagttggcgc acatgcgcaa
    25501 ggtcgcgccc atctggtgga acgaacagcc gaagggtgtc ggctgtttcg acgacgacgg
    25561 cttttgggtg ctgaccaagc acaaagacgt caaagaagtg tcgctgcgca gcgacatctt
    25621 ctccagcaat gcaaagaccg cgctgccgcg ctaccgcgag ggcgccgacc ccgagtcgct
    25681 cgagaggggc aaggtcgttc tactcaacca ggacgccccg caccacaccc acctgcgcaa
    25741 gatcatctcg cgggcgttca ctccccgcgc catcgagcgg ctgcgcgacg agctcaacga
    25801 gcgggcaaag aacatcgtca aggcggccac cgccgagggc tcgggcgact tcgtcgaaca
    25861 ggtgtcctgc gagcttccgc tgcaggcgat cgcgggattg atgggtgtcc cccaggacga
    25921 gcgcaagaag cttttcgact ggtcgaatca gatggtcggc gaccaggacc cggaattcgc
    25981 caacaatgac ccgatggccg cctcgatcga gctgatcatg tacgggatgc agatggctgc
    26041 agaacgcgcc aagaaccccg gcgaggatct cgtgaccaag ctggtgcagg cggacgtcga
    26101 aggtcacaag ctcaccgacg acgagttcgg cttcttcgtc atcctgcttg ccgtcgcggg
    26161 caacgagacg acgcgcaact cgatcactca aggcatgatg gcgttcaccg aatttccgga
    26221 ccagtgggag ctgttcaaga aggaacggcc ggccaccgcc gccgatgaga tcgtccgctg
    26281 ggccaccccg gtcacctcgt tccagcgcac cgcgctcgaa gacaccgagt tgtcaggcgt
    26341 gaagatcaag aagggtcaac gggtggtgat gttttaccgc tccgcgaact tcgatgaaga
    26401 cgttttcgac gatccgttca ggttcaacat cctgcgagat ccaaacccgc acgtgggttt
    26461 tggcggcacc ggcgcgcact attgcgtcgg tgcgaacctg gcacggatga cgatcgatct
    26521 gatcttcaac gcgatcgccg acgagatgcc caatctcaca ccgatttcgc agccggagcg
    26581 gctacggtcg ggctggctga acggcattaa acactggcag gtcgactatc tcggccggtc
    26641 gaaacagccg gtcgcgcagt cgtcgggtag tcgatgatcg agcgccagta gtcctctggt
    26701 atctcgtcgc ctgtccgcag caccatcgca aggccggtgg ccatcgcgat gccgagcaac
    26761 ccgagccaca gtccggccag gccgatgatc gcccagcaca ccgcggtcag cgtcggccac
    26821 ggtgacacca caacaagcac gggcgcgaag aagaagcccg cgccgatgta ccaggccgac
    26881 ccggcgcggt ccgagatcag cacgaaacgc agccaccggg ggatgttctg ctcggtcctc
    26941 attcctcgac tctcccccta ccctgcgggt gggaggtgcc cccacctcgc tccggtcctc
    27001 gctcgttcct cgctgcgatc ctcactcgtt gtcgtcttcg gtcatgtctc cacggtgccg
    27061 cggccaggta agcggcgcaa ttacctgaga ggtagtggct gcggtccccc ggtgtgcgca
    27121 acactgggcg cgtgaccagc gtcgcccaag tccagagccc acccttcggc tcgctgatgc
    27181 gtcagtggcg gcagcgccgc cggctgtcgc aactcgatct cgcgatcgag gccgacgtgt
    27241 cggcccgcca cgtcagtttc atcgagaccg gccggtcggc tcccagccgt gagatggtgt
    27301 tgcggctcgc gggcgtgctc gacgtgccgc tgcgcgaaca gaaccaactg ctgatggcag
    27361 ccgggctggc gccggtctac cgcgagcgct cactcgccga tcccgacatg gcggccgtgc
    27421 gggaaggcgt cgagcgagtt ctcaacgcct acaacccatt tccgtgcgtg gtggtcgacc
    27481 gcggctggca gctcgtgcat gccaacgccg gtgccgcggt gctgctcgac ggcgtggcac
    27541 cgcatcttct ggagcggccg aacgcgctgc gcatcgcggt gcatcccgaa gggttggcgc
    27601 cgcgcatccg caacctcggg cagtggcgtc atcacctgat cgaacggctg cggcgcgagg
    27661 cggccgtgag cggctcggcc gagctgtcgg cgctgctggc cgagatcgag tcgtatccgg
    27721 gcggtttgga cccgacgcgc gatctcggcg gcgtcgcggt tcccctcgag ctttacacgt
    27781 ccgacggaaa gctattgacg ttcctgagta ccgtcacgac gtttggcacc gcgttggatc
    27841 tcaccgccgc agagctcagc atcgaggcgt tccttcccgc cgacgaggcg acggcttcgg
    27901 cactgcgcgg gtaaaacacc cgtgggagcg gccaaaaatc aatagagtct ggcgacatgc
    27961 agacccgaca catagcggtg gccgctgcgg tgctcgttgt gggattggcc ggctgcggtg
    28021 ggcagccagc agcactcggc ggcaccaccg cgaaggtgac catcgatggc aaggacaccg
    28081 gcaatccgca cgcggtgagg tgctcccaaa cggggtgggc gtggaccatc gaaaccgcgg
    28141 gcaaggacaa gggtttcacc gccgtgctcg gcaccgggga caaggtggcc gtcaaatcgg
    28201 tcgacttccg tgacttcgcg ggcttttccg gcaccacctg gggcgacatc ggcaaggccg
    28261 aggtgaccgg tgtcggcggg aagtacacga tcaccggctc cgccgacgga aacttcaccg
    28321 ataacccgag caaggacgtc accgcgacct tccggatcga agcaaactgc tgagtggcgc
    28381 acccattccg tcgcgcgatt gaagcacgtg atctcgaggc ggccgtcgcc ctgctacggg
    28441 aagacgtggt ctttcggagc ccagtcgtct tcaagccgta ccacggtcgc gaggcgctgc
    28501 gcctgatact tcaggctgtc ctggcggtgt tcgaagactt ccactatgtg cgcgagatcg
    28561 gcgccgacga cgcccgcgat cacgccctca tgttcgaggc gcgcgtcggc gataagcgca
    28621 tcgagggatg tgacttcatc cggctcgatc aggacggcgc gatcagcgag ttcacggtga
    28681 tggtgcgccc gatgtcgggc atgctcgcac tcgccgaggc gatgaaggtg cagctctcgg
    28741 cgttggagta actggtcaga ccacttgacc tctggccaat tgcctggcag gcttggctgc
    28801 atggccttgc acccggtgaa ccgccgctcc gtgcccgagg acgtcttcga gcagatcgtt
    28861 gccgaggtgc tcagcggtga catgaagccc ggtgaggccc tgcccagcga gcgccgcctg
    28921 gccgaggtcc tcggggtctc ccgcccggcc gtccgcgaag cgctcaagcg gctcaccgcc
    28981 gcgggtctgg tggaggtgcg tcaggggggc gccactaccg tgcgcgactt ccaccggcat
    29041 gcgggactcg acctgctgcc ccggttactg atccgcgatg gcgagctcga tctctcggtg
    29101 gtgcgcagca tcctcgaaac ccggctgcac aacggaccca aggttgccga gctagcggcc
    29161 cggcgccggc gcccggaact ctccgcacag ttcgacgacg tgctgcagaa gctggcggcc
    29221 gagggcgacc cggtggagca gcagcgcctc gcgctgacgt tctgggatca cgtcgtcgat
    29281 ggcgccgact cgatcgcgtt ccggctgatg tacaacacgc ttcgcgccac ctatgagccc
    29341 gcactcgccg cgctggccac tgtgatggcc gacgaggtgg gccggcctgc ggcctaccgc
    29401 gaactcgccg acgcgatcat caccggtgat cccacgcggg cgagcaaagc cgcgcacgat
    29461 ctgctcgagc ccgcgaccac cgggctgctg accgcgatcg acaccttgga ggaacaacga
    29521 tgaccaccgt gaccgagcgc ccaacccgaa aggggttcac gctcgccgac gcaagccgtg
    29581 aattctggaa acaaccgtcc ccctggatga ttggcgcgac gtttgtcgtc gcgctgaccg
    29641 cgcgcctcaa tgttggcgac tggcagatca ctgatgcgct cgtgccgatc gtgatgatcg
    29701 cgttgttccc gctagtcgag tggatcatcc acgtgtgcat tctacattgg cgcccaaagc
    29761 atctcggtaa gctcaccatc gatccattgc tcgcccggga acaccgggca caccacacgg
    29821 acccacggaa cgttcggttg atattcatcc cgtggaagtc gctcgcgttc tgggtgcttc
    29881 cgctcgctgt tgccgttgcg ctgttggcat ttccgcggct cgggatggga cttacctacc
    29941 tggtgtttct gtcagcgctg gggctgggtt acgagtggac ccactacctc atccacagca
    30001 actacaagcc caaaacccgt gtctatcgcg ctatctggcg caaccaccgc aatcaccact
    30061 acaagaacga gcactactgg ttcacggtga ccagctcggg taccgccgac cgggtgctcg
    30121 gcacgtaccc cgatcctgcc accgtcgcaa cgtcaccgac cgcgaagaac ctgcacgcta
    30181 ctgctgaacg atgatcgggt ccccgatgtg cacggtgtcg taataccagg ccgcgttgtc
    30241 ggggcccaga ttgatacaac cgtggctgac gttcgaatta ccttgtgagc ccaccgacca
    30301 cggggcggag tgcacataga tgccgcccca cgtgacgcgc acggcgtcgt tgacggtgag
    30361 cttgtagccc tccgggtcgc tcagcggaat gccgatggtc cgtgaatcca tcaccacgac
    30421 cgactgcttc tcaagcgccg cgaacgttcc aaccggtgtt gggaacctgg acttacccat
    30481 cgatgcgggc atctggcgca ccacctgacc gtcaatgctg acggtgaagg tatgcgcgtc
    30541 gatgtcggcc acccccactg tggcggcgcc cgtctcgaat gacgttttcg cgccacccgc
    30601 cgtcacgatg atgttcgagt gagcgggcca gaatccggtc ggattccatt gcacgacacg
    30661 gtcattgagc caagtaaacg aaccggcagg gatggcggag gaggtgaacg agatggtgcg
    30721 ctcggcggcg gcgcggttgc tgatcgactg cgcgaaggtc acggtcaccg gcatcgcgac
    30781 acccaccttc tggccctgcg ccggttgaat gttcacaacg tcggtcggcg cggtgacagc
    30841 ggccgaaccg gtggcgacgg atcccgcagt cgcgatcact gcaatgcccg ccacgacaag
    30901 cacattgcga acggctctac gcacagtctg cattcctccg agatgggctg tcagcacacc
    30961 gcaatcgtat cgggtcagct ccgaataccc gggtaaccgc aagtcagagc ccccgtgcgc
    31021 atgtcgcgct acagcacggt cgccactgcg cgattgatta ccagcaacgt gtagccaagc
    31081 atgagcgcac caccggccag cacacaaatg ttcatcacga cagtcgccgt tccgacggtg
    31141 tcggggtcga gaaagtagta cggggcacgg cggcccgcgt tgttgagcac aaccagtgcc
    31201 agtgccaggt atgccgtcgg gtaggcgagc caggccaccg gctgccacca gcggacctgc
    31261 gcttgatccc gcccgacaag cagccagtcg gtcagtgcca gcaccggcac gacgacgtgt
    31321 agcagcatgt tggccggtgt gtagcccatg ctgtaacccg tcaggaacag attccagatg
    31381 acgccggcga tgagcacgta cagcaccacg gcgccccgga taccgaccct ggcatccgcg
    31441 cggggccggg cgagggtcca caggtagtac gccgcggcga gcacgttgac ctgatatgtg
    31501 aacgtgctca gccgccacag cagacctgag cgagtcgatg tcgcgacggc gaccagcgca
    31561 agcagaacgg ccagcacgat cgcgacccga agcgtcactc gc
//
LOCUS       DASRJA010000446         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_217776,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000446 DASRJA010000000
VERSION     DASRJA010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217776"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1643)
                     /locus_tag="VFC01_19590"
     CDS             complement(273..1643)
                     /locus_tag="VFC01_19590"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZN81866.1"
                     /translation="MPREGDFITRPDTNYTYMHGSPWPYDVSIPVMFAGPAVKGGVYS
                     GPATQQDVAPTLAAALGVRMPPTATGRVLPVFRNDFVRPRAVMLLVLDGMRRDYFDRY
                     ATLMPTFTALRQRGAWFSNAEVSILPTNTAVGHSTIATGADPSVHGITGVSLYDFRNG
                     RRQDLFAGTAPKDLMALTLADVWQLATAGRAIILAQGSIDRAATPLAGHGACQFNGTN
                     VVLASYDQEDGHWKSSATCFRLPEYLKDRNASDLWAASAEWMGHRIDSPIAVRYSALF
                     PAFEVDAMSTMIARERVGVDGVPDLILMNYKGADFVGHKYGPDSEELRVTLVEMDLQL
                     ARLLGALEAEVGNDYLLAVTADHGMPPEPPSSDRRHFVPDLVDLLHQRFDPQAKKLIT
                     SFDPENCQIFVDEGRVAELGLTLRDLAEFLESQPFIFAAFTNDDAFRARANDPRVATV
                     PYAAGR"
     gene            1984..>2403
                     /locus_tag="VFC01_19595"
     CDS             1984..>2403
                     /locus_tag="VFC01_19595"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN81867.1"
                     /translation="MPWLETAPVQQRERFIHDRRLNLYTMTELCARYGVSRKTGYKWL
                     GRFDAGGRQGLQDRSRAPRHCPHRIAEETARLICAARRGHPSWGPDKLLDWLAPRHPG
                     VDWPAVSTAGDLLARRGLVKKRRRRRHYEHPGVVPPTT"
BASE COUNT          448 a          784 c          748 g          423 t
ORIGIN      
        1 gcacccgatc attctctaac ttgaggcgga cactatctga gttaactttc agggtgtctt
       61 gggcttctgc gacagctgcc ccgtactgtg ccgaaagcgc aacgaacaac accgctagcc
      121 agatgttggt cttcatctca acctcctgtg caacaacagt agaacaacac gcctaacgcc
      181 cggcgtcagc ggcggcgcgg accgccgtcc gctgggatcc gttgttgggc ggccttgtgc
      241 ctccttgctg gcagtcactc agccttctct gtctatcgcc cagctgcata cgggaccgtc
      301 gctactcggg ggtcgttggc ccttgcccgg aatgcatcgt catttgtgaa tgccgcaaag
      361 atgaacggct gtgattcgag gaactctgcg agatcgcgca acgttagacc aagctcggcc
      421 acgcgaccct cgtccacgaa tatctggcaa ttctccgggt cgaaggacgt gatgagcttc
      481 ttggcctgtg gatcgaatct ctgatgcaac aggtcgacga ggtcaggcac gaagtggcgc
      541 cgatccgatg acggtggctc cggtggcatg ccgtgatcgg cggtcaccgc gagcagataa
      601 tcattcccga cctctgcctc caacgcgccc agcagccgcg ccaattggag atccatttct
      661 acaagcgtga cgcggagctc ttcggaatca gggccgtact tgtggcccac aaaatcggcg
      721 cccttgtagt tcatgagaat caaatccggg acgccgtcca cacccactcg ttcgcgggcg
      781 atcatcgtgg acatcgcatc gacttcgaat gccggaaaca gtgccgagta tcgcaccgcg
      841 atgggcgagt cgattcgatg gcccatccac tcggcgctgg ctgcccacag atcgctcgcg
      901 ttgcggtcct tgaggtactc cggcagccga aagcacgtgg cgctcgactt ccagtggccg
      961 tcctcctggt cgtagctggc gagtacgacg ttggtgccat tgaactggca cgccccatgc
     1021 ccggcgagag gcgtggccgc tctgtcgatg ctgccctgcg ccaggatgat cgcccggccc
     1081 gccgtggcga gctgccacac gtcagccaga gtcagtgcca tcagatcctt cggtgccgta
     1141 ccggcgaaga ggtcttgccg ccgcccgttc ctgaagtcat acaggctgac gcccgtgatt
     1201 ccgtgcacac tcggatcggc ccccgtcgcg atggtcgagt gccctacggc ggtgttcgtc
     1261 ggcaggatgc tgacctctgc gttgctgaac caagccccgc gctggcgcaa ggcggtgaac
     1321 gtaggcatca gtgtggcgta acggtcgaag tagtcccgac gcatcccatc caggacgagc
     1381 agcatgaccg cacgcgggcg gacgaaatca tttcggaaca ccggcagcac gcgaccggtc
     1441 gccgtggggg gcatccgcac gccgagtgca gccgcgagcg ttggagcgac gtcctgctgt
     1501 gtggccggcc ccgagtacac tccgcccttc accgcagggc cagcgaacat caccggaatc
     1561 gaaacgtcat acggccacgg tgagccgtgc atgtacgtgt agttcgtgtc aggccgggtt
     1621 atgaaatctc cctcgcgtgg cacgaccaag agctgaccgg ttcgtccggg atagtacgcc
     1681 cgggcccaca tctcgagaaa gcgctgtcgc gtcgctggcg ccgaagtatc gtccggcaca
     1741 gtgcgaacgc ccgaagacac gcagccactt agagccagcg ccagtacgag cacgactgtg
     1801 ctgcgacagc ttggtagctt caaccagcgg ccaggggtgc ccttcgttct catggctact
     1861 cgagcttcca gtagttgcct gccccggtgc ggcagcttgg tacaaaccgg gtacaagggt
     1921 aacagaacaa accgggaaca agggtgacac cactacgctg gggagatcaa cggaggttga
     1981 tcgatgccct ggctggagac cgcacccgtg cagcaacgcg aacgcttcat tcacgatcgc
     2041 cgactgaacc tctacaccat gaccgagctg tgcgcgcgct acggcgtcag ccgcaagacg
     2101 ggttacaagt ggctgggccg tttcgacgcc ggtggacgcc aggggctgca ggaccgcagc
     2161 cgcgcgcccc ggcattgtcc gcatcgcatc gcggaggaga cggcgcgact catctgcgcc
     2221 gcgcggcgcg ggcacccgag ctggggaccc gacaagcttc tggactggct cgcgccccgg
     2281 catccggggg tcgactggcc ggccgtgagc acggccggtg atctcctggc gcgacgcggc
     2341 ctggtcaaga aacggcggcg ccggcggcac tacgagcacc ccggcgtcgt cccgccgacc
     2401 acc
//
LOCUS       DASRJA010000447        11110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4036362,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000447 DASRJA010000000
VERSION     DASRJA010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4036362"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFC01_19600"
     CDS             <1..450
                     /locus_tag="VFC01_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HZN81868.1"
                     /translation="RLPALRPDQLVILGPRDDAWRRQFNVGTLADSRVWLAPLAEVAD
                     DPAGTGRQAIEQLTKHVDRWWLHIDLDVLDPVEFPAQGLPDVADEPGGLTWDQLTDLV
                     SALFTRPGSCVGGSLAIYDPDQDADGTGAAQIVAFIRNVLVRPTISP"
     gene            458..2641
                     /locus_tag="VFC01_19605"
     CDS             458..2641
                     /locus_tag="VFC01_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZN81869.1"
                     /translation="MAEPIDEHFTATISALSGSAAERSHEDALRDGSRLTVGECLALF
                     DAQLGSRHLDLAARWLRSKGKGYYTIGSSGHEGNAALAAALRPTDPALLHYRSGGFFL
                     ARAAQVGGRDPLRDVLLGLVAATDEPIGGGRHKVFGRRDLNIIPQTSTIASHLPRAVG
                     VAFSIARTRKLGVACPWPDDAVTVCSFGDASVNHSTAVGAINTALHSAYQGVPMPLLL
                     VCEDNGIGISVKTPRRWIEHTYGNRDGLQYFDADGWDSAATFDAASAAAAWVRAHRRP
                     AFLHLRMVRLMGHAGSDYEPAYRRPDEITADFDRDPVLCTAKALIDAGALTPEQALDR
                     YEAKRANVIELAGEVADLPQLDTAAAVMKPLQDVLEDAVAAAPASLAATVRSGEPDAP
                     LTLALAINRALHDILDRYPEAMIFGEDVARKGGVYGVTRGLLGKTSSAQVFDTLLDEQ
                     AILGLALGAGVSGLLPIPEIQYLAYVHNAADQIRGEGATLQFFSNRQYRNPMVVRVAG
                     YGYQKGFGGHFHNDDSIAALRDIPGLVIASPARPDDAAAMLHTCAAAANAAGVVCVFL
                     EPIALYHTRDLYQEGDDGWLAAYPVEGVPIGRARTYGEGTDLTILTFGNGLRMSLRVA
                     RKLESLGVRARVVDLRWLAPLPVSDMIREASATGRVLIVDETRATGGVGEGVLAELLE
                     HGYTGAVDRVASSDSFIPLGDAALQVLLSEDTIEAAAIKLAGPGA"
     gene            2654..3859
                     /locus_tag="VFC01_19610"
     CDS             2654..3859
                     /locus_tag="VFC01_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN81870.1"
                     /translation="MSVAPDAARPLDGVRIVEISSFVAVPLAGMTLGQLGAQVIRVDP
                     IGGAADYHRWPVTGDGESIYWAGLNKGKRSVAADFRSDEGQDLVQRLIVDSGVLITNV
                     AGRQWHSYETLTRLRPDLIHVEVSGRADGGTGVDYTVNAGIGFPMVTGPAELATPVNH
                     VLPAWDVACGIYAALAVVTALRHRDATGQGQQISIPLENVALATAGNLSLFTEVMVNG
                     TARERIGNSVYGQYGQDFTSSDGVSFMIVALTGRHFRDLTELTGTTKAVAALADTLGA
                     DFTDEGERFRHRDALSGLFAEWFSQHSAVEVSAGLSATSVLWERYRTFSDVVTEGRLT
                     GNPMFSSLDQPRIGQYLAPGLPMEIDGEYPPAVAAPALGDDTTAVLGEWLGMSADEIG
                     RLTDSGTVA"
     gene            3856..4656
                     /locus_tag="VFC01_19615"
     CDS             3856..4656
                     /locus_tag="VFC01_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HZN81871.1"
                     /translation="MSALLKLLDLKPGADADTWLGPASGPAGKRAYGGQFVAQSLAAA
                     LNTVDADRPPTNLHLQFLRGGEAGDPIEYTVTRVFDGRTAAARRVDSHQDGRLLTTAS
                     VSFAAELPGLQHGHRAAMPGDPDSLPRTGPAGPAPSMPLNELDIRIDDDISTGAFVRR
                     LWWRATVPLPDDPTVHTLVAAYVTDVYMIDPALQVHGHSMKARTHRSGTTDSAIWFHR
                     PVRADEWNLLECRSPAAARGRGVVTASLIRADGAIAATLTQEGLIAER"
     gene            complement(4549..7071)
                     /locus_tag="VFC01_19620"
     CDS             complement(4549..7071)
                     /locus_tag="VFC01_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN81872.1"
                     /translation="MSCRLLDHLRRYDDNVAVLTDTQQVTYHELADLVADTARELGGP
                     RRLVLLETRNDLGTLVHYLAAFSGGHVVLPVSAGRDHAAITQTYTPDVVIDANGIHGG
                     GRRDRLHDDLALLLSTSGTTGSPKLVRLSYGNLVSNAAAIAEYLDIRETDRAATTLPM
                     SYCYGLSVIHSHLLRGAGLILTDRSVIDDEFWELFRRHGGTAFAGVPYTFEMLEHIGA
                     DALDLPHLRYVTQAGGRMPPERVKLFADLGRQQGWDLFVMYGATEATARMSYLPPQLA
                     SSHPAAIGRPIPGGAFTIEPNGELVYRGPNVMMGYAHRAADLALGKTVDALHTGDIAR
                     RGPDGLYEVIGRRSRFVKMYGLRIDLQRVEANLREHGVTAFCTDVDDRLAVAAAAHDE
                     AEVQRVTAAAAGVPADAVKAVTVAELPILPSGKPDYQAVRDLAHTSNTNEPNVIGLRE
                     LFADVLQIDTDSIDPDASFVDLGGNSLSYVTMSVRLERALGRLPADWQRLSVRELEAT
                     PKPLRRRWPWWGATLETSVALRAAAILLIVGSHAGLFELWGGAHLLLGIAGYNFGRFC
                     LTPVPRTDRVRHLRNTIAWIAAPSVAWIVIALMITDDYAPTNLLLANKFLGPHDSMTA
                     GRLWFVEVLVWTLVALAVLCWLPLVDRWERQWPFAVAVAFLAAGLALRYDVLGLHLGR
                     DAWFTVLAFWFFAAGWAAAKASTALQRAAVTVVLIIGLHGYFDRTEREMLVLAGFALL
                     IWLPALRCPPAATVVAGVIAEASLYIYLTHYQVYPLFDGHPLLGVIASIIVGVLLTQL
                     VSTVRRRLTLGDKPFLGQGRRDGAVGADQACGDDTAAARRSR"
     gene            complement(7068..8117)
                     /locus_tag="VFC01_19625"
     CDS             complement(7068..8117)
                     /locus_tag="VFC01_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN81873.1"
                     /translation="MTTTILEIKGLAKSFNAHTVLHDIDLALQPGTTTAVVGSSGCGK
                     TTLLRLIAGFESPDAGTITIDGRQVASPESAVAPHRRSVGYVAQDGALFPHLTVGQNI
                     AYGLRGTTRGEMRTRVGELLETVSLDPSYASRRPHQLSGGQQQRVALARALARHPVLM
                     LLDEPFSALDTGLRASTRKVVSGLLADAGVTTLLVTHDQEEALSIADQVAVMRDGRFT
                     QVGRPQQVYREPVDRFTAEFLGDCISLPCTVASGVADCALGRIPVQGSAADGPTTLVL
                     RPEQLVATVVSDSEHLDGVGTVIATEFLGHDVLLTIDPAGDAAPLIVRQHSLNPPPID
                     AKVRIEVVGTGIVLE"
     gene            complement(8114..9688)
                     /locus_tag="VFC01_19630"
     CDS             complement(8114..9688)
                     /locus_tag="VFC01_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZN81874.1"
                     /translation="MVAPPVTRPTAPVSRTDKAARPGPLVTATVAFLVAATLIPLGYV
                     AWAAISIGWTRAYELVVRPRVGELLFNTVALVVITVPLCVVIGVGVAWLVERTDLPAR
                     GFWRPLFVAPLAVPAFVNSYAWVGVIPSLHGLWAGVLVTTLSYFPFMYLPVAATLRRL
                     DPAVEESARALGSDSTGVFLRVVLPQLRLAILGGGLLIGVHLLAEFGAFAMVRFDTFT
                     VAIFQQFQVTFDGAAGSMLAGVLVLLCLVLLVAEASARGHARFSRIGAGAPRAATPIR
                     LGRNMLPAQAGLVALAVLALGVPVWTIMRWLWIGGADVWVLDDVATAFGQTVGLAAIA
                     AVLTTVLAFPVAWVAVRSSGVLARVVEGANYVTSSLPGIVTALALVTVTIHAARPLYQ
                     SVALIVFAYMLLFMPRALVNVRAGLAQVPPSLEEASRSLGSSPTATFLRVTLRLTAPA
                     AAAGASLVFVAVATELTATLLLAPTGTRTLSMRFWSFSSELDYAAAAPYALVLVVLAI
                     PVTLVLFRQSTKVAAL"
     gene            complement(9689..10717)
                     /locus_tag="VFC01_19635"
     CDS             complement(9689..10717)
                     /locus_tag="VFC01_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HZN81875.1"
                     /translation="MRRRWSRIASPIVIVVSALAVVLGATACSSSGESDELLIYNAQH
                     ESLTKEWIDAFTKETGIKVTYRQGGDTELGNQLVAEGAASPADVFLTENSPAMAAVER
                     AGLFTDLGAETINQVPAQYRPASGKWTGVAARTTVFVYNKAKLQPDQLPKSLLDLQQP
                     AWKGRWGAPPTKADFQAIVSALLQLKGEAATAQWLAGMKANAKTYNDNIATLKAVNSG
                     EVDGGVIYHYYWFRDQAKTKEMSGNTALHYFRNQDPGAFVSLSGGGVLKSSKKADKAQ
                     QFLRFITGKAGQEVLEKGDSFEYPVASGVPANPALVPLTDLQAPAVDPSTLNAQKVTD
                     QMTKAGLL"
BASE COUNT         1874 a         3624 c         3812 g         1800 t
ORIGIN      
        1 cgcctgcccg cgttgcggcc cgaccagctg gtgatcctcg gtccgcgcga cgacgcgtgg
       61 cggcggcagt tcaacgtcgg cacgctcgcc gattcccggg tgtggctggc cccgctcgcc
      121 gaggtcgccg acgaccccgc cggcaccggg cgtcaggcca tcgagcagct gacgaagcac
      181 gtggaccggt ggtggctgca catcgacctc gacgtgctcg accccgtcga gtttcccgcc
      241 cagggattgc ccgacgtcgc ggacgaaccg ggcggcctga cctgggatca gctcaccgac
      301 ctggtctcgg ccctgttcac cagaccgggg tcgtgcgtcg gcggcagcct tgcgatctac
      361 gacccggacc aggacgccga cggcacaggc gctgcgcaga tcgtcgcctt catccgaaac
      421 gtgctggtac gtccgacaat aagcccctag gagcccggtg gccgaaccga tcgacgagca
      481 tttcaccgcg accatttctg cactcagcgg atccgcggcc gaacgctccc acgaggatgc
      541 gctgcgcgac ggatccaggc tgaccgtggg cgagtgtctg gcgttgttcg acgcgcaact
      601 cggcagccgg cacctcgacc tggccgcccg ctggttacgg tcaaagggca agggctacta
      661 cacgattggg tcctcggggc acgaagggaa cgccgccctc gccgcggcgc tgcggcccac
      721 cgatccggcg ttgctgcact atcgctccgg cggtttcttt ctagcccgcg ccgcccaggt
      781 cggcggacgt gacccgttgc gggacgtgct gctcgggctg gtggcggcga ccgatgagcc
      841 gatcgggggc gggcggcaca aggtgttcgg ccgccgcgac ctgaacatca ttccgcagac
      901 ctcgaccatc gcatcgcacc ttccgcgggc tgtcggcgtc gcgttctcca tcgcacgcac
      961 aaggaaattg ggcgtggcct gcccatggcc ggacgacgct gtgaccgtgt gtagtttcgg
     1021 cgacgcgtcg gtgaaccact cgaccgccgt cggcgccatc aacaccgcgc tgcactcggc
     1081 atatcagggc gttccgatgc cgttgttgct ggtctgcgag gacaacggga tcggcatcag
     1141 cgtcaagacg ccacgacggt ggattgaaca cacttacgga aaccgtgacg gactacagta
     1201 tttcgatgcc gacggctggg attcggcggc gacgttcgac gccgcatctg ccgctgctgc
     1261 gtgggtgcgt gcacaccgac ggcctgcgtt cctgcatctg cggatggttc ggctgatggg
     1321 acatgccggt tcggactatg aaccggccta tcgtcgtccc gacgagatca ccgccgattt
     1381 cgaccgggat ccggtgctgt gcaccgcgaa agcgttgatc gacgccggtg cgctgactcc
     1441 cgaacaggca ctggaccgtt atgaggccaa gcgtgccaac gtgattgagc tcgcaggtga
     1501 ggtcgcggac ctgccgcagc tcgacaccgc tgcggcggtg atgaagcctc tacaggacgt
     1561 cctcgaggac gccgtcgctg cggcccccgc gtcgctggcc gcgacagtgc ggtccggtga
     1621 gcctgacgcg ccgctgacgc ttgcactcgc gatcaaccgt gcgctgcatg acatcctgga
     1681 ccgctacccg gaggcgatga tcttcggcga ggatgtcgcg cgcaagggcg gcgtgtacgg
     1741 cgtcactcgt ggcctgctcg gcaagacgag ttcggcacag gtgttcgaca cgctcctcga
     1801 tgagcaggca atcctcggcc tggcgctcgg cgcaggggtt tctgggctgc tgccaattcc
     1861 ggaaatccag tacctggcct acgttcacaa cgccgcggac cagatccgcg gcgagggtgc
     1921 gacgttgcag ttcttctcca accggcaata ccgaaatccg atggtggttc gggtggccgg
     1981 ctacggatac cagaaaggct tcggcgggca cttccacaac gacgactcca tcgcggcgct
     2041 gcgcgacatt ccaggactcg tgattgcgtc gcccgcgcgg ccagacgatg ccgcggcgat
     2101 gctccatacc tgcgcggctg cagcgaacgc ggccggtgtc gtgtgcgtct tcctcgaacc
     2161 gatcgcgcta taccacaccc gcgatctgta tcaggagggc gacgacggct ggctcgcggc
     2221 gtatccggtc gaaggcgtgc cgatcggccg ggcgcgcacc tatggcgaag ggacggacct
     2281 gacgatcctc acgttcggca acgggctacg gatgagcctg cgggtggccc ggaagctgga
     2341 atctctgggg gtgcgggcgc gtgtcgtcga cctgcggtgg ctggcaccgc tgccggtgtc
     2401 cgacatgatt cgcgaggcca gcgccaccgg acgagtgctg atcgtcgacg agacccgcgc
     2461 taccggtgga gtgggagaag gcgtgctcgc cgagctgctc gagcacgggt acaccggcgc
     2521 tgtggatcgg gtggccagca gcgacagctt catcccgctg ggtgacgccg ccttgcaggt
     2581 attgctctcg gaggacacca tcgaagccgc agcgatcaaa ctggccggtc cgggcgcttg
     2641 atagcttgag cgcatgagcg ttgccccgga tgccgcccgg ccgctggacg gggtgcggat
     2701 cgtcgagatc tcgagcttcg tcgcggtgcc gttggcgggg atgacgctgg gccagctcgg
     2761 ggcccaggtg atccgcgtcg acccgatcgg cggcgccgcc gactaccacc gctggccggt
     2821 cacgggcgac ggcgagagca tctactgggc gggcctgaac aaaggcaagc gctccgtggc
     2881 ggccgacttt cgctcggacg aaggccagga cctcgtgcag cggctgatcg tcgacagcgg
     2941 ggttctgatc acgaatgtcg ccggacggca atggcattcg tacgagacgc tgacccggct
     3001 gcggcccgac ctgattcatg tcgaagtgtc cggtcgcgcc gacggcggta cgggcgtgga
     3061 ctacaccgtc aacgcgggca tcgggtttcc aatggtcaca ggccccgccg agctcgcgac
     3121 accggtgaat cacgttctgc ccgcgtggga cgtcgcgtgc gggatttacg cggcgctggc
     3181 cgtcgtcacc gcgttgcggc atcgcgacgc cacggggcag ggtcagcaga tcagcattcc
     3241 gctggagaat gtggcgctgg ccaccgccgg caatctcagc ctcttcaccg aagtcatggt
     3301 caacggcacc gcgcgcgagc gcatcggcaa ctcggtgtac ggccaatacg gccaggactt
     3361 caccagtagc gacggcgtgt cgttcatgat cgttgcactg acgggacggc acttccgcga
     3421 cctgaccgag ctcactggaa caacgaaagc cgttgccgcg ctggctgaca cactcggcgc
     3481 cgacttcacc gacgagggcg agcggttccg ccatcgtgac gcgctgtccg gcttgttcgc
     3541 cgagtggttt tcgcaacaca gtgccgtgga ggtcagcgcg gggttgtccg cgacctcggt
     3601 gttgtgggag cgctaccgca ctttctccga tgtggtcacc gaagggcgac tgactggcaa
     3661 cccgatgttc agctctcttg accagccccg gatcgggcag tacctggcgc ccgggctgcc
     3721 gatggagatc gacggcgagt acccgccggc agtcgctgcg ccggcgctcg gcgatgacac
     3781 caccgcggtg ctcggcgaat ggctgggcat gtctgcggac gagatcggcc ggctcacgga
     3841 ttcgggcaca gtggcgtgag cgcgctgctc aagctgctcg acctgaagcc gggcgcggac
     3901 gcggatacgt ggctcggtcc cgccagcggt ccggccggaa agcgtgccta cggagggcaa
     3961 ttcgtcgcgc agagcctcgc cgcggctcta aacacggtcg acgctgaccg gccgccgacc
     4021 aacctgcatc tgcagtttct gcgcggcggc gaggccggcg acccgatcga atacacggtg
     4081 acccgggtgt tcgacggcag gactgccgcc gcgcgccgcg tcgactcgca tcaggacggg
     4141 cgcctgctca cgaccgcctc ggtgtcgttc gccgccgagc ttcccggcct ccaacacggg
     4201 caccgggccg ccatgccggg cgatcccgac tcgctgccgc gcaccgggcc ggccggacct
     4261 gcgccgtcga tgccgttgaa cgagctggac atcaggatcg acgatgacat ctcgactggc
     4321 gcgttcgtgc ggcgattgtg gtggcgggca acggttccgt tgcctgacga tccgacagtc
     4381 cacacgttgg tcgcggcgta cgtcaccgac gtctacatga tcgaccctgc gctgcaagtg
     4441 cacggccatt cgatgaaagc ccgcacccat cgcagtggca ccaccgactc ggcgatctgg
     4501 ttccatcgcc ccgttcgcgc cgacgaatgg aatctgctgg agtgccgctc accggctgcg
     4561 gcgcgcggcc gcggtgtcgt caccgcaagc ctgatccgcg ccgacggcgc catcgcggcg
     4621 accttgaccc aggaagggct tatcgccgag cgttaggcgc cttcggaccg tcgagacgag
     4681 ttgggtcagc agcacaccga cgatgatgga cgcgatgacg cccaacagcg ggtgaccatc
     4741 gaacagcggg tacacctggt aatgcgtcag gtagatgtac agcgacgcct ccgcgatgac
     4801 cccggcgacg acggtggccg cgggcgggca gcgcagcgcg ggtagccaga tcagcagggc
     4861 aaacccggcc agcaccaaca tctctcgttc agtgcggtcg aagtagccgt gcaggccgat
     4921 gatcagcacg acggtcaccg ccgcacgctg cagcgcggtc gacgccttgg ccgcggccca
     4981 gcccgccgcg aagaaccaga aggccagcac ggtgaaccat gcgtcgcggc cgaggtgcag
     5041 tccaaggacg tcgtaccgca gcgccagtcc ggcggccagg aacgccaccg cgacagcgaa
     5101 cggccactgc cgttcccacc ggtcgacgag cggcagccag cagagtacgg ccagcgccac
     5161 gagagtccac accaggactt cgacgaacca cagtcggcct gcggtcatgc tgtcgtgcgg
     5221 gccaaggaac ttgttggcca gcaacagatt cgtgggggca tagtcgtcgg tgatcatcag
     5281 cgcgatcacg atccacgcca ccgatggtgc cgcgatccac gcgatggtat tgcgcagatg
     5341 ccgcacccga tcggttcggg gcaccggcgt gaggcagaac cggccgaagt tgtatcccgc
     5401 gatgcccagc aggagatgtg cgccgcccca cagttcgaac agcccggcgt gcgatccgac
     5461 gatgagcagg atcgcggcgg cgcgcaaagc gacgctggtc tccagcgtcg cgccccacca
     5521 cggccaccgc cgtcgcaacg gtttgggcgt ggcctccagc tcgcgcacag acagtcgctg
     5581 ccagtctgcg ggcaaccggc ccagcgcgcg ttccagccgg accgacattg tcacgtagga
     5641 cagcgaattg ccgcccaggt cgacaaagct ggcgtcgggg tcgatgctgt cagtatcgat
     5701 ctggagaacg tcggcgaaca gctcgcggag accaatcaca ttgggctcgt tggtgttcga
     5761 cgtgtgtgcg aggtcgcgca ccgcttggta gtccggcttg cctgacggca gaattggcag
     5821 ctccgcgacc gtgacggcct tcaccgcatc ggcaggcaca ccggcagcgg ccgcggtgac
     5881 gcgttgaacc tcggcctcgt cgtgagcggc agcggccacc gcgagccggt cgtcgacatc
     5941 ggtgcagaac gctgtgacac cgtgctcccg caggttcgcc tcgacgcgtt ggaggtcgat
     6001 ccgcagcccg tacatcttga cgaatcggct tcggcggccg atcacttcgt acagcccatc
     6061 gggtccgcgg cgggcgatgt ctccggtgtg cagcgcgtcg acggtcttgc cgagcgcgag
     6121 atcggcggcc cggtgtgcgt agcccatcat cacgttgggg ccgcggtaca ccagctcgcc
     6181 attcggttcg atcgtgaacg cgccgccggg gattggcctg ccgatcgcgg cggggtgcga
     6241 cgaggcgagt tgcggtggca gataggacat tcgcgctgtg gcttccgtcg ccccgtacat
     6301 gacgaacaag tcccagccct gttgccgacc gaggtccgcg aacagcttca cgcgctcggg
     6361 cggcatccgg ccgccggcct gggtgacgta ccgtagatgc ggcaggtcca aggcgtcggc
     6421 gccgatgtgc tccagcattt cgaaggtgta ggggacgccg gcgaacgcag tcccgccgtg
     6481 tctgcggaaa agctcccaga actcgtcgtc gatgacggac cggtcggtga ggatcaggcc
     6541 ggcgccgcgc agcaggtggc tgtggatcac cgagaggccg tagcagtacg acatcggcag
     6601 ggtggttgcg gccctgtcgg tttcgcggat gtcgagatat tcggcgatgg ccgcggcgtt
     6661 ggacaccaga ttgccgtagg acagccgcac cagcttgggt gagccggtgg tgccggaggt
     6721 ggacagcaac agcgcgaggt cgtcgtgcaa tcggtcgcgt cggccgccgc cgtgaattcc
     6781 gttggcatcg atcaccacat cgggcgtata cgtctgcgtg atcgcggcgt ggtcgcgtcc
     6841 ggcggagacg ggcaagacca cgtggccgcc cgaaaacgcc gccagatagt gcaccaaagt
     6901 gcccaagtca ttgcgagttt cgagcaacac caggcggcga ggcccgccga gttctcgcgc
     6961 ggtgtcagcg acgaggtccg cgagttcgtg atatgtgacc tgctgggtgt cggtcagcac
     7021 cgccacgttg tcgtcgtagc ggcgcaagtg gtcgaggagt cggcagctca ttcgagcacg
     7081 atcccggtgc ccaccacctc gatccgcacc ttggcgtcga tgggcggtgg gttgaggctg
     7141 tgctggcgga cgatgagcgg ggcggcgtcg ccggccgggt cgatcgtcaa caacacatcg
     7201 tggccgagga actcggtggc gatcaccgtc cctacgccgt cgaggtgttc actgtcggaa
     7261 accaccgtcg cgacaagctg ttcgggtcgc agcacgaggg tggtgggtcc gtcggcggcc
     7321 gagccctgca cggggatgcg gccgagtgcg cagtcggcga cccccgatgc gaccgtgcat
     7381 ggcagggaaa tgcagtcgcc gaggaactcg gcggtgaagc ggtcgacggg ttcccggtac
     7441 acctgctgcg gacgaccgac ctgcgtgaac cggccgtcgc gcatgaccgc cacctgatcc
     7501 gcgatcgata gcgcctcttc ctggtcgtga gtcaccaaaa gcgttgtcac gccggcgtcg
     7561 gcgagcagac cggaaaccac cttgcgggtg gacgctcgca gcccggtgtc gagcgcgctg
     7621 aacggttcgt cgagcagcat cagcaccgga tgccgggcga gcgcacgcgc cagcgcgacg
     7681 cgctgctgct gaccgcccga gagttggtgc gggcggcgcg acgcgtatga cgggtcaagg
     7741 gaaacggttt cgagaagttc accgacgcgg gtacgcattt cgccgcgggt ggtgccgcgc
     7801 aatccgtatg cgatgttctg accgacggtc aggtgcggga acagcgcacc gtcctgtgcg
     7861 acgtagccga cacttcggcg gtgcggggcc acagcacttt ccgggctggc gacttgccgt
     7921 ccgtcgatgg tgatcgttcc ggcgtcgggt gactcgaatc cggcgataag ccgcagcagt
     7981 gtggtcttgc cgcaacccga cgatccgacg acggcggtcg tggtgcccgg ttgcagggcg
     8041 aggtcgatgt cgtgcagcac ggtgtgcgcg ttgaaggatt tggcgagtcc cttgatctcg
     8101 aggatggtgg tcgtcatagc gcggccacct tcgtcgactg ccggaacagc accagggtca
     8161 ccggaatcgc gagcaccacc agcacgagcg cgtagggggc ggcggccgcg tagtccaact
     8221 cgctggaaaa cgaccagaac cgcatcgaca gcgtcctggt tccggtcggc gcaagcaata
     8281 gtgtcgcggt caattccgtt gccacagcga cgaataccag cgaagcgccc gcagccgcgg
     8341 ccggtgcggt cagtcgcagt gtgacccgaa ggaaggtggc tgtcggcgag ctgcccagcg
     8401 accgggacgc ctcttcgagg ctcggcggca cctgagcgag gccggctcga acgttgacca
     8461 gcgctcgcgg catgaacagc agcatgtagg cgaacacgat caacgcgacg ctctggtaga
     8521 gcggacgtgc ggcgtggatg gtgaccgtga cgagcgcgag ggcggtgacg atgcccggaa
     8581 gcgagctggt gacgtagttg gcgccttcca cgacgcgggc gagcacaccg ctggatcgca
     8641 cggcgaccca cgcgacgggg aacgcgagca ccgtggtgag tacggcggca atggcagcca
     8701 gtccgacggt ttggccgaac gcggtggcga cgtcgtcgag cacccagacg tcggcgccgc
     8761 cgatccacag ccagcgcatg atcgtccaca ccggcacgcc gagcgccagc accgccagcg
     8821 caaccagacc agcttgagca ggtaacatgt tgcggcccaa gcggatcggg gtagcggcgc
     8881 gcggggcacc ggctccgatc cgcgagaaac gggcgtgacc gcgcgctgaa gcctcggcca
     8941 ccagtagcac caggcagagc agcacgagga cgccggcgag catgctgcct gccgcgccgt
     9001 cgaaggtgac ctggaactgc tggaagatcg cgactgtgaa ggtgtcgaac cgcaccattg
     9061 cgaatgcgcc gaactcggcg agcagatgca caccgatcag cagtccacca ccgaggatgg
     9121 cgagccgcag ctggggcaac accactcgga ggaacacgcc ggtcgagtcg gaacccagtg
     9181 cccgcgcgga ctcctcgacc gcgggatcga gccgccgcag cgtcgcggcc accggcaggt
     9241 acatgaacgg gaagtacgac agcgtcgtca ccaacacgcc ggcccacagc ccatgcagcg
     9301 acgggatcac gccgacccac gcgtagctgt tgacgaacgc cgggacggca aggggcgcaa
     9361 cgaacaacgg gcgccagaac cctcgtgcgg gtaaatcggt ccgctccact agccacgcca
     9421 caccgacacc gatgaccaca cacagcggca cggtgatcac gaccagggcc acggtattga
     9481 acagcagctc gccgaccctc ggccggacta cgagttcgta tgcgcgcgtc cacccgatcg
     9541 atatcgcggc ccacgcgacg taaccgagcg gaatgagggt ggcggcgacc aggaatgcga
     9601 cggtggcagt gaccagcggg ccgggtcggg ctgctttgtc agtccgcgag accggcgccg
     9661 tcgggcgcgt aaccggggga gcgaccacct ataacaaccc agctttcgtc atctggtccg
     9721 tcaccttctg cgcgttcagc gtcgaaggat ccactgcggg cgcctgcaag tcggtgagcg
     9781 ggacgagtgc ggggttcgcc ggtacaccgc tggccaccgg atactcgaac gaatcgccct
     9841 tctccagcac ttcctgcccg gccttgccgg tgatgaaccg caggaactgc tgcgccttgt
     9901 ccgccttctt gctggacttg agcacaccgc cgccggagag gctgacgaac gcgccgggat
     9961 cctggttgcg gaagtagtgc agcgccgtat tgccgctcat ctctttggtt ttcgcttgat
    10021 cgcggaacca gtagtagtga tagatcacgc cgccgtcgac ctcgccggag ttgaccgcct
    10081 tgagcgtggc gatgttgtcg ttgtaggtct ttgcgttggc tttcatcccg gccagccact
    10141 gcgcggtggc ggcctcgccc ttgagttgca gcagcgcgga cacgatggcc tggaagtccg
    10201 ccttcgtcgg cggtgcgccc cagcggccct tccatgccgg ctgctgaaga tcgagcagcg
    10261 acttgggcag ctggtcgggt tgcagcttgg ccttgttgta gacgaacact gtcgtgcgcg
    10321 ccgcgacgcc ggtccactta ccgctggcgg ggcggtactg cgcgggaacc tgattgatgg
    10381 tctcggcgcc gaggtcggtg aacagcccgg cccgctcgac ggcggccatc gcgggggagt
    10441 tctcggtcag gaagacgtcg gcgggcgacg cggcaccttc agccaccagc tggttgccga
    10501 gttcggtgtc cccgccctgc cggtaggtca ccttgatgcc ggtctccttg gtgaacgcgt
    10561 cgatccattc cttggtcagc gactcgtgct gggcgttgta gatcagcagc tcgtcggact
    10621 cgccggaact agagcatgcg gttgcaccga ggacgacggc gagggctgac accactatga
    10681 ctattgggct ggcgatccgg ctccaacgtc gtcgcatcca gtcaagtcct tccctatcca
    10741 cgcctttagc tgagctttac ctaagggaac ttacccttcg ctgactttca tctgatgccg
    10801 tgccgaagat ttggttcgca tttggccgga gggtaggcct tctgaccgcc catgccagtt
    10861 ggtagctgcg gcggagcgtg acgaggttct tacgagattg tcaacgtcgg attgatcttg
    10921 aagcgctgcg ggtacgtttg ccctcaggtc aggggttgcg tgacccattg gcaacaacat
    10981 ctgaggacaa ccgaaatgac catgtcagtt actgccgtgc gccgcggcct gttcggcgcg
    11041 tttgccacat gcgcgctcgg tggcctcgcc gcagcgacga tcgcgattcc atcggcgggt
    11101 gcagcgcccg
//
LOCUS       DASRJA010000448        14997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3878636,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000448 DASRJA010000000
VERSION     DASRJA010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14997
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3878636"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VFC01_19640"
     CDS             <1..918
                     /locus_tag="VFC01_19640"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HZN81876.1"
                     /translation="AGAGGRDAAVIDIAQDLLLRDLHTRDALEAIVFKGGTALRKLYA
                     GNQGRFSVDLDFSLARASDDSEAVVLDLIAHIEGTTIGPFTYGVVERRGKWSLTIQSP
                     FSSGEPSLSSKLDISPPVWLNPVHRGWVPMPVHNTYGQPPLPELSVIRLEENIAEKIA
                     RLNRVTTARDMYDLAWIAQHQHDVGSLDLNLIRRLAVLKIWVDSNGLAGGKAHWRQGH
                     KPCEFDPTSWLRTRDVSEFDLEDIGALAIPTPSAAELAYVVRTRYRFLSDLDDDELKL
                     AAASEKDRRLALKLLERLPGGRLADIGIY"
     gene            1029..1361
                     /locus_tag="VFC01_19645"
     CDS             1029..1361
                     /locus_tag="VFC01_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN81877.1"
                     /translation="MLRITTLVGTAVAASALGVAALASTGVASAATSVDDTFISVITD
                     EGIKPPSAKEAIGVAHDVCVVLDKGGDLVDAVSAVADYTELESEDAAFFVGASIAAYC
                     PEYEELIG"
     gene            complement(1358..2464)
                     /locus_tag="VFC01_19650"
     CDS             complement(1358..2464)
                     /locus_tag="VFC01_19650"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HZN81878.1"
                     /translation="MTDGPLEFTVERNANPVSDEVRASILNDPPFGQFHTDHMVSIDY
                     VDGKGWHNARVIPYGPIELDPSAIVLHYAQEVFEGLKAYRWADGSIVSFRPEANARRL
                     RTSAARLAIPELPEEAFIESLRQLIAVDQPWVPPAGGEESLYLRPYIFATEPGLGVRP
                     ASEYRYLVIGSPVGAYFKGGIKPVSVWLSTEYVRACPGGTGAAKFGGNYAASLLAQAQ
                     AIENDCDQVVWLDAIERRYVEEMGGMNLFFVFGSGGSARLVTPELSGSILPGVTRDSL
                     LQLATDAGFAIEERRIDVDEWQKKVATGEITEVFACGTAAVITPVARVKSADGEFTIG
                     DGEPGEISMALRDTLTGIQRGTFADTHGWMARLS"
     gene            complement(2487..3581)
                     /gene="gcvT"
                     /locus_tag="VFC01_19655"
     CDS             complement(2487..3581)
                     /gene="gcvT"
                     /locus_tag="VFC01_19655"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZN81879.1"
                     /translation="MSEVLHGPLEERHRQLGASFAEFSGWLMPVSYAGTVSEHTATRT
                     SVGLFDVSHLGKALVRGPGAAEYVNSALTNDLRRIGPGKAQYTLCCTDSGGVIDDLIA
                     YYVSDDEIFLVPNAANTPAVVAALQERAPAGLTITDEHRSYAVLAVQGPKSTDVLGGL
                     GLPTDMDYMGYADAEFGGVPVRVCRTGYTGEHGYEVLPPWDQADVVFDALVDAVGSAG
                     GELAGLGARDTLRTEMGYPLHGHELSLDISPLQAHCGWAIGWKKEAFWGRDALLAEKE
                     AGPKRVLRGLRAVGRGVLRPDLTVLDGDTPIGVTTSGTFSPTLKIGIALALLDTDKDV
                     ADGQTVSVDVRGRGLECEVVKPPFIEAKTR"
     gene            3596..4714
                     /locus_tag="VFC01_19660"
     CDS             3596..4714
                     /locus_tag="VFC01_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN81880.1"
                     /translation="MRGVRAGRLRWVVDFEALEAAGIANARERAGLIEYLDGLGFTAD
                     DMIEAERHGRLFGLAGDALQGSGRPVYTLHAAAETLGLPDGDLAHIWEALGLTVADPQ
                     QPTLSQSDVDGLAAWVTLRTVTGEDAALNFLRVVGATMARLAEASGTMIRIAQPELMI
                     TYTHDELTTARAYRAVAELVPGLSTLMDSVFRHQITSARAYFEGVISDTSANVTCGVG
                     FADLSGFTSLTQRLTPTELSDLLVEFGGAVSDVVHADGGRVVKFIGDEVMWVTATPER
                     LAKVAVDLVEYPRAREAGLQVRAGLGYGSVLALGGDYWGTPVNLAARLVAAAAPGQIL
                     ASTDVRDELPDWPAIPLDPLTLKGFESPVMAYDLHLSR"
     gene            4733..6256
                     /locus_tag="VFC01_19665"
     CDS             4733..6256
                     /locus_tag="VFC01_19665"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZN81881.1"
                     /translation="MSTANPGYQAPAVTVASALPKRKKDSTVLIVPVVSSQDDDATAT
                     VVSNPFLDAAAVGEIDVALEALGAKGGTDQVTRVVAPSLPVGSVVAVGLGKERDTWPA
                     DVIRRAAGAAARSLNGTEAVITTLSDLDLSAAIEGLILGAYRFTDFRSSKTAPKDAGL
                     REITALTKDTKAKSKTAAERATAIATAVATARDLVNTPPSHLYPDEFAKRAKALGEAA
                     GLEVEVLDDKTLAKDGYGGVIGVGKGSSRPPRLVRLAHRVAKKKAKKVALVGKGITFD
                     TGGISIKPAANMHHMTSDMGGAAAVIATVVLAAKQQLPIDVIATVPMAENMPSSTAQR
                     PGDVLTQYGGITVEVLNTDAEGRLILADAIVRACEDKPDYLIETSTLTGAQTVALGAR
                     TPGVMGSDEFRDRVAEISQAVGENGWPMPLPEELKDDLKSTVADLANVSGSRYAGMLV
                     AGTYLREFVADGVQWAHIDIAAPAYNTGGPWGYTGKGATGVPTRTMFAVLEDIAANG"
     gene            complement(6258..8021)
                     /locus_tag="VFC01_19670"
     CDS             complement(6258..8021)
                     /locus_tag="VFC01_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN81882.1"
                     /translation="MGEISERFIDSTDGVRIAVYEEGNPDGPTVVLVHGWPDSHVLWN
                     GVVPLLADRFRILRYDNRGAGKSTVPKKVSAFTMHCFANDFAAVIDALAPGQPVHVLA
                     HDWGSAGVWEYLSRAGASDHVASFSSLSGPSADHLVMYLRNSLLRPYRPKRFARALNQ
                     ALRLTYMGVFSVPLLLPLAMRAFLADAIRRLVIARGIPSHQLHYADTFKADAANGMKI
                     YRANFFRSTGNVRRDHYVDVPVQLIVNTKDNWIQPHGYDDTHNWVPRLWRRDIKAGHW
                     SPMSHPRVLARSVEQLVDFLDGKPAARELLRAQVGRPREYFGDTMVSVTGAGSGIGRE
                     TALAFAREGAELVISDIDEATVKETAAQIAARGGVAHAYTLDVSDADAVEQFAEQVSA
                     EHGVPDIVVNNAGVGQAGLFLDTPREEFDRVLATNFGGVVNCCRSFGRRLVDRGTGGH
                     IVNISSMAAYSPQQSMNAYSTSKAAVFMFGDCLRAELDQAGVGLTTVCPGLINTNIVH
                     TTRFDVPDGKQAKVEARRAQIKKAFAARRYGPDKVAKAIVSAVKKNKPIRPVAPEAYF
                     VYGVAHLLPQAMRSSARASVM"
     gene            complement(8060..8413)
                     /locus_tag="VFC01_19675"
     CDS             complement(8060..8413)
                     /locus_tag="VFC01_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZN81883.1"
                     /translation="MGLFDRFRGGRRARGSAGNDPAADLRYLQQWVAEHTGVEAFVEP
                     RTTVTDVTVVLVAADGEWTRRRAGGDAGARRLSDRLKIPVYDVQKVGYPQRMRDYDAR
                     RRIERERALREELEG"
     gene            8558..10357
                     /gene="sucB"
                     /locus_tag="VFC01_19680"
     CDS             8558..10357
                     /gene="sucB"
                     /locus_tag="VFC01_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HZN81884.1"
                     /translation="MAISVQMPALGESVTEGTVTRWLKQEGDTVEQDEPLLEVSTDKV
                     DTEIPSPAAGVLKKIIAQEDDTVEVGGELAVIGDASEDGESSGGQVKAAEKPAEEEEK
                     PAEEPAAQPEPAAEEKEEPAEAPKPAAQSSGGGQATSVKMPELGESVTEGTVTRWLKK
                     VGDSVEVDEPLVEVSTDKVDTEIPSPVAGTLVSITAEEDDTVAVGGELAKIGEAGAEA
                     AEAPAPEPKPEPEPEPKEEAKPEPKPEPKPEPKPEPKPEPEPAQEAKPEPKPEPKPQP
                     EPSPTSAEAGPYVTPLVRKLAAENDIDLASVKGTGVGGRIRKQDVLAAAEAKKAPAAP
                     AREEAPAAAAKPAAAAATPAPALAHLRGTTQKASRIRQITAKKTRESLQATAQLTQTH
                     EVDMTKIVALRAKAKNGFAEREGVNLTYLPFIARAVIDALKLHPNVNASYNEETKEIT
                     YYDAEHLGFAVDTEQGLLSPVIHNAGDLSLGGLARAIADIAARARSGNLKPDELSGGT
                     FTITNIGSQGALFDTPILVPPQAAMLGTGAIVKRPRVIVDENGNESIGIRSVSYLPLT
                     YDHRLIDGADAGRFLTTIKRRLEEGAFEADLGL"
     gene            10364..11275
                     /locus_tag="VFC01_19685"
     CDS             10364..11275
                     /locus_tag="VFC01_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HZN81885.1"
                     /translation="MTNAVIAIAGSSGLIGSALVYALRATDRKVLRIVRRAPSNADEV
                     FWNPDTGEFDPSTLAGVDAVVNLCGINVGEKRWSGAFKQSLRDSRIGPTEVLSSAVVE
                     AAVPTLINASAVGYYGDTNSQTVDETAPAGEGFMAQLCEDWEAATSFAAHDGTRVVLV
                     RTGLVLAPAGGILGRIKPLFSLGLGARLGNGRQYMPWISLEDEVRALLFAISHDDVSG
                     PVNLTGPAPVTNAEFTAALGRTVNRPTPLVAPGFALRAVFGEFAEEGLLGGQRAIPAV
                     LERAGFHFHHNTIGEALAFATAKNHEG"
     gene            11286..11957
                     /gene="lipB"
                     /locus_tag="VFC01_19690"
     CDS             11286..11957
                     /gene="lipB"
                     /locus_tag="VFC01_19690"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HZN81886.1"
                     /translation="MAASIRSATAPIDIRRLGTVDYQTAWQLQRELADARIAGGPDTL
                     LLLEHPSVYTAGRRTLPEERPVDDTPVVDTDRGGKITWHGPGQLVGYPIVGLAEPLDV
                     VNFVRRLEESIITACAGFGLETGRIEGRSGVWVPSDGVRPARKVGAIGIRVSRSTTLH
                     GFALNCDCDLSAFSSIVPCGISDAGVTSLTAELGRRVSVADLLGPVADAVCDALDGRL
                     VVALT"
     gene            11957..12889
                     /gene="lipA"
                     /locus_tag="VFC01_19695"
     CDS             11957..12889
                     /gene="lipA"
                     /locus_tag="VFC01_19695"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZN81887.1"
                     /translation="MTVAPEGRKLLRLEARNAETPIERKPPWIKTRAKMGPEYKELKA
                     LVRREGLHTVCEEAGCPNIFECWEDREATFLIGGEQCTRRCDFCQIDTGKPAELDRDE
                     PRRVAESVQAMGLRYSTVTGVARDDLPDGGAWLYAETVRAIKKLNPNTGVELLIPDFN
                     GEPTLLEQVFEARPEVLAHNVETVPRIFKRIRPAFRYERSLNVLTAARDYGLVTKSNL
                     ILGMGETPDEVRTALRDLYDAGCDIVTITQYLRPSVRHHPVERWVKPEEFVEHEEFAK
                     GLGFTGVLAGPLVRSSYRAGRLYAQAVASRPAVS"
     gene            12894..13646
                     /locus_tag="VFC01_19700"
     CDS             12894..13646
                     /locus_tag="VFC01_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HZN81888.1"
                     /translation="MAKQRDPAAAKAAKAEAKVTRKAASKQRRSQLWQAFQIQRKEDK
                     RLLPYMIGAFVLIVGASTAFGIISGGFTGYMMIPLGIVLGALVAFIIFGRRAQKSVYR
                     KAEGQTGAAAWALDNMRGKWRVTPGVAATGHFDAVHRVIGRPGVIFVGEGAANRVKPL
                     LAQEKKRTARLIGDIPIYDIMVGNDEGDVPLAKLERHLNKLPANITVKQMDSLESRLA
                     ALGTKAGPAAMPKGPLPTQAKMRNVQRTVRRR"
     gene            complement(13647..14120)
                     /locus_tag="VFC01_19705"
     CDS             complement(13647..14120)
                     /locus_tag="VFC01_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZN81889.1"
                     /translation="MARAIGSWLSGPGPNSGSQDKGPNDHPGQRLGLPRTGPRSLARM
                     GRRIAALFVDWLIAYGLAALAMSFGLVSMTSLSTAVLLIWFVLGAVSVRLFGFTPGQF
                     ALGLMVVPVDGRQHVGFGRAVVRGILIAVVIPALITDVDWRGLQDKVTNTAVVRR"
     gene            14281..>14997
                     /locus_tag="VFC01_19710"
     CDS             14281..>14997
                     /locus_tag="VFC01_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HZN81890.1"
                     /translation="MAEKTADDILKQIKDDEVEYVDIRFCDLPGIVQHFSVPASAFTE
                     DVFEDGLAFDGSSVRGFQSIHESDMLLLPDPETARIDPFRAAKTLNLNFFVHDPFTRE
                     AYSRDPRNIARKAENYLISTGIADTAYFGAEAEFYIFDSVSFDSKMNGTFYEIDSEAG
                     WWNTGEQFEPDGSPNRGYKVRPKGGYFPVAPYDHYVDLRDQMCTNLQNAGFTLERGHH
                     EVGTAGQAEINYKFNTLLHAA"
BASE COUNT         2730 a         5044 c         4848 g         2375 t
ORIGIN      
        1 gctggcgctg gcggccgcga cgccgccgtc atcgatatcg cgcaggacct tctgcttcgt
       61 gacttgcata cgcgcgacgc actggaggct attgttttta agggcgggac tgccctgcgc
      121 aagttgtacg caggaaacca aggacgattc tcggtcgatc tcgacttcag tctcgcccga
      181 gcgagcgacg actcggaggc cgtagtcctc gacctaatcg cccacatcga aggcacaacc
      241 atcggtccct tcacttacgg cgtcgtcgag cggcgaggca agtggtcgct gactatccag
      301 agcccgttca gcagcgggga accatcgcta tcaagcaagc tcgatatctc gccgccggtc
      361 tggctcaatc cggtgcaccg ggggtgggtg ccgatgcccg tccacaacac ttatggacag
      421 ccacccctcc ctgagctttc ggtcatcaga ctggaggaga acattgcgga gaagatcgcc
      481 cgcctcaatc gggtgactac agcgcgcgac atgtatgacc tggcctggat cgctcagcat
      541 caacacgacg ttggcagcct tgatctcaac ctcatccgtc gtcttgctgt actcaagata
      601 tgggtcgatt ccaacggact ggcaggagga aaggcccact ggagacaagg acacaaaccg
      661 tgtgagttcg accccacctc ttggttacgg acccgcgacg tcagcgaatt cgacctcgag
      721 gacattggcg cacttgctat cccgacacca tctgccgccg agttggcata tgttgtccgc
      781 actcgctatc gcttcctctc cgatctcgac gacgacgagc tcaagctcgc tgcggccagt
      841 gagaaagacc gtcgcctcgc tctaaaactc ctagaacgac ttccgggcgg ccggctcgca
      901 gatatcggca tttattgacg cgctggcgat ccgttagctg gccgccaagg atgcgccaag
      961 aatccgccaa ggcctgagcc ggaatctttc ggtgtccgca agggacaacg acaaccgaaa
     1021 ggattccaat gctccgcatc accaccctgg tcggcaccgc agtcgccgcg tctgctcttg
     1081 gcgtcgccgc gctcgcctca acgggcgtcg cctcggccgc cacctccgtc gacgacacct
     1141 tcatcagcgt catcaccgac gagggcatca agccgccgtc agcgaaggaa gcgatcggtg
     1201 tcgcccacga cgtgtgcgtc gtgctcgata agggcggcga cctggtcgac gcagtcagcg
     1261 cagtcgcgga ctacacagag ttggagagcg aggacgcagc gttcttcgtc ggtgcctcca
     1321 tcgcggccta ctgcccggag tatgaggagt tgatcggcta agagagccgg gccatccagc
     1381 cgtgcgtgtc ggcgaacgtg ccgcgctgga tgccggtcaa cgtgtcgcgc agggccatcg
     1441 agatctcacc gggttcgcca tcgccgattg tgaattcgcc gtcggcggac ttcaccctcg
     1501 ccactggggt gatgaccgcc gcggtgccac atgcgaacac ttcggtgatc tcccctgtcg
     1561 caaccttctt ctgccattcg tcgacgtcga tcctgcgctc ctcgatcgca aacccggcgt
     1621 cagttgctaa ctgcaacaat gaatctcgcg tgacaccggg caggatcgac ccggacagct
     1681 cgggagtcac caaccgcgcc gaaccgccgc tgccgaacac gaagaacagg ttcatcccgc
     1741 ccatttcttc gacgtagcgg cgttcgatgg cgtccaacca caccacctga tcgcagtcgt
     1801 tctcgatggc ctgggcctgc gccagcagcg acgcggcgta gttgccgccg aatttggcgg
     1861 cgccggtgcc gccgggacag gcgcgcacat attcggtcga cagccatacg ctgaccggct
     1921 tgatgccgcc cttgaaatac gcccccacag gcgacccgat gaccagatac cggtattcgc
     1981 ttgccggccg cacacccagc cccggctcgg tggcgaagat gtatggccgc agatacagcg
     2041 actcctcgcc gcccgcgggc ggcacccatg gctggtcgac cgcgatcagc tgacgcagcg
     2101 actcgatgaa cgcctcttcg ggcagttcgg ggattgccag ccgggccgcc gaggtgcgca
     2161 accgcctggc gttggcctcc ggccggaagg agacgatgga gccgtccgcc cagcggtagg
     2221 ccttgagccc ttcgaacacc tcctgggcgt agtgcagcac gatggccgac gggtccagtt
     2281 cgatcggccc gtaggggatc acgcgggcgt tgtgccagcc cttgccgtcg acgtagtcga
     2341 tcgacaccat gtggtcggtg tggaactgac cgaacggcgg atcgttcaga atcgacgccc
     2401 gtacctcgtc gctcaccggg ttcgcgttgc gctcaaccgt gaactcgagg gggccgtcag
     2461 tcatgcagcg attgtatatc tgctcgctac cgggtttttg cctcgatgaa cggcggtttg
     2521 acgacttcac attcgaggcc gcgaccgcgc acgtccaccg agacggtctg cccgtcggcg
     2581 acgtccttgt cggtgtcgag caacgccagc gcgatgccga ttttcaaagt cggagaaaag
     2641 gttcccgacg tcgtcacgcc aatcggtgta tcaccgtcga gcacggtgag gtccgggcgc
     2701 aacacgccgc gaccgacggc acgcagccca cgaagcaccc gcttcgggcc cgcttccttc
     2761 tcggccagca gcgcgtcacg tccccagaac gcctctttct tccacccgat cgcccatccg
     2821 caatgtgcct gtagcggcga aatgtccagt gacagttcgt ggccgtgcag cggatagccc
     2881 atttcggtgc gcagcgtgtc gcgggctccc agcccggcca gctcaccgcc tgcgctgccg
     2941 accgcatcga caagtgcgtc aaaaaccacg tcggcctgat cccacggcgg cagcacttcg
     3001 taaccgtgct cgcccgtgta gccggtccgg cacacccgga ccgggacacc gccgaactct
     3061 gcgtcggcgt accccatgta atccatgtcg gtgggtaggc ccaaaccgcc gaggacatcg
     3121 gtcgatttgg gcccctgcac ggcgagcacc gcatacgagc ggtgttcgtc ggtgatcgtc
     3181 aggcccgcag gcgcgcgttc ctgcagtgcg gcgacgacgg ccggcgtgtt ggccgcgttg
     3241 ggtaccaaga agatctcgtc gtcggacacg tagtaggcga tcaggtcgtc gatgacgccg
     3301 ccggaatccg tgcagcacaa cgtgtattgg gccttacccg gtccgatgcg gcgaaggtcg
     3361 ttggtgagcg cggagttgac gtactcggcc gcgcccggtc cgcgcaccaa cgccttgccg
     3421 aggtggctga cgtcgaacag cccgacgctc gtgcgcgtcg cggtgtgttc actgacggtg
     3481 ccggcgtagg agaccggcat cagccagccg gagaactccg cgaaactcgc gccgagttga
     3541 cgatggcgct cctcgagggg gccatgaagg acttcgctca cgccgaaaca ccctaatgcg
     3601 aggcgtccgc gctggcagac tccggtgggt ggtcgatttc gaggcgctgg aagccgcagg
     3661 aatcgccaat gcccgcgagc gtgccggcct gatcgagtac ctggacggcc tcggcttcac
     3721 cgcagacgac atgatcgagg ccgagcgcca cggacgcctg ttcggcctgg ctggagacgc
     3781 tctgcaggga tctggccgcc cggtttacac cctgcacgcc gccgccgaga cgctcggtct
     3841 gcccgacggt gatctcgcac acatatggga ggcgctcgga ctgacggtgg ccgatcccca
     3901 gcagccgacc ctgagtcaat ccgacgtcga cgggctggcg gcgtgggtca cgctgcggac
     3961 ggtcaccggc gaggacgcgg cgctcaactt cctgcgggtg gtcggcgcca cgatggcccg
     4021 gctcgccgag gccagcggaa cgatgattcg gatcgcccag cccgagctga tgatcacgta
     4081 cacccacgac gaactcacca cggctcgcgc gtaccgtgcc gtggcggagc tggtgcccgg
     4141 tctatccaca ttgatggact ccgtttttcg ccaccagatc accagtgccc gagcctattt
     4201 cgagggagtc atcagcgaca cgtccgcaaa cgtcacgtgc ggcgtcgggt tcgcggatct
     4261 gtccggcttc acgtcgctga cgcagcgact caccccgacc gaattgtctg atctgctcgt
     4321 cgagttcggt ggcgccgtca gcgacgtagt gcacgccgac ggtggacggg tggtgaagtt
     4381 catcggcgac gaggtgatgt gggtgaccgc tacccccgag cggctcgcca aggtagcggt
     4441 cgatctcgtc gagtacccac gagcgcggga ggccgggctg caggtccgcg ccggcctggg
     4501 ctacggctcg gtgctggctc tcggtggcga ctactggggc accccggtga atctggcggc
     4561 ccgcctggta gcggcggcgg caccgggcca gatcctggca tcaaccgacg tccgcgacga
     4621 gctgcccgac tggcccgcga tcccgctgga tccgttgaca ctcaagggtt tcgagtcgcc
     4681 cgtcatggcc tacgacctgc acctgtcccg ctgacggcta gggtaaacgg tcgtgagcac
     4741 cgcaaacccg ggttatcagg cccccgccgt caccgtcgcg tcggcgctac ccaaacgcaa
     4801 gaaggattcg acggtgctga tcgtgccggt cgtcagcagc caggatgacg acgcgacggc
     4861 gacggtcgtc agcaatccct tcctcgacgc ggcggccgtc ggcgagatcg acgtcgcgct
     4921 cgaagcgctc ggcgcgaaag gcggcaccga ccaggtcacc cgggtggtcg ccccgtcgct
     4981 gcccgttggc agcgtggtcg cggtcgggct gggcaaggag cgcgacacat ggcccgccga
     5041 cgtcatccgc cgcgccgccg gggcggccgc acgctcactc aacggcacgg aagcggtgat
     5101 caccacgctg tcggacctcg acctgagcgc agcgatcgag ggcctgatcc tcggcgccta
     5161 ccgcttcacc gacttccgca gctccaagac cgcacccaag gatgccggcc ttcgcgagat
     5221 cacggcgctg actaaagaca ccaaggccaa gagcaagacg gcggccgagc gcgcgacggc
     5281 gatcgccacc gctgtcgcca ccgcgcggga tcttgtcaac actcccccgt ctcacctcta
     5341 tcccgacgaa ttcgccaagc gcgcaaaggc tttgggcgag gcggctggcc tcgaagtcga
     5401 agtactcgac gacaaaacgc ttgccaagga cggctatggc ggcgtgatcg gtgtgggcaa
     5461 gggctcgtcg cgtccgccgc ggctggtccg gctcgcccac cgcgtggcca agaagaaggc
     5521 gaagaaggtc gcactcgtcg gcaagggcat cacgttcgac accggcggca tctcgatcaa
     5581 gccggcggcc aacatgcacc acatgacctc ggacatgggc ggtgccgccg ctgtcattgc
     5641 aaccgtcgtg ctggccgcca aacagcagct gcccatcgac gtgatcgcga cggtgccgat
     5701 ggccgagaac atgccgtcgt ccaccgcgca gcggcccggc gacgtgctca ctcagtacgg
     5761 cggcatcacc gtcgaggtgc tcaacaccga cgcggagggc cggctgatcc tggccgacgc
     5821 tatcgtgcgg gcctgcgagg acaagccgga ctacctgatc gaaacgtcga cgctgaccgg
     5881 cgcgcagacg gtcgcgctgg gcgcgcgcac gcccggcgtg atgggcagcg acgagttccg
     5941 cgaccgcgtc gcggagattt cgcaggccgt cggcgagaac ggctggccga tgccgctacc
     6001 cgaggagctc aaggacgacc tgaaatcgac ggttgccgat ctggccaacg tcagcggctc
     6061 gcgctacgcg ggcatgctgg tcgccggcac gtacctgcgc gagttcgtcg ccgacggggt
     6121 gcagtgggca cacatcgata tcgcggcgcc ggcgtacaac accggcggac cgtggggcta
     6181 caccggcaag ggagccacag gcgtgcctac gcgcacgatg ttcgcggtgc tggaagacat
     6241 cgctgcgaac ggctgaccta catgacgctg gcgcgtgccg aactgcgcat cgcctgcggc
     6301 agcaggtggg cgacgccgta gacgaaatac gcctccggcg cgacgggccg gatcggcttg
     6361 ttcttcttga ccgccgacac gatcgccttg gccaccttgt ccggtccgta tctgcgggcg
     6421 gcgaacgcct ttttgatctg agcccgccgc gcctccactt tggcctgctt gccgtcgggc
     6481 acgtcgaacc gggtggtgtg cacgatgttc gtgttgatca gcccggggca caccgtcgtg
     6541 aggccgaccc cggcctgatc gagttcggcg cgaaggcagt cgccgaacat gaacaccgcg
     6601 gccttgctgg tggagtacgc attcatcgac tgctgcggcg aatatgcggc catcgacgaa
     6661 atgttgacga tgtgcccgcc ggtgcctcga tcgacgagtc ggcggccaaa tgacctgcag
     6721 cagttgacga ctccgccgaa gttggtggcg agtacccggt cgaactcttc gcgcggggtg
     6781 tcgaggaaca gcccggcctg ccccacgcct gcgttgttga ccacgatgtc gggcacgccg
     6841 tgttcggcgc tcacctgctc agcgaactgc tcgacggcgt ccgcatcaga gacatcgagt
     6901 gtgtaggcat gtgccacccc gcctctggcg gcaatctgcg cggcggtctc cttgacggtg
     6961 gcctcgtcga tgtcgctgat caccagttcg gcaccctcgc gggcgaaggc cagcgcggtc
     7021 tcacggccga tgccgctgcc agcgccggtc acggaaacca ttgtgtcgcc gaagtattcg
     7081 cgcgggcgac cgacctgcgc gcgcaacaac tcacgcgccg caggcttacc gtcgaggaag
     7141 tcgacgagct gctcgacaga ccgggcgagg acccgcggat gcgacatcgg cgaccagtga
     7201 ccggccttga tgtcgcgccg ccacagccgc ggcacccagt tgtgggtgtc gtcgtacccg
     7261 tggggctgaa tccaattgtc cttggtattg acgatcaatt gaacgggaac atcgacgtag
     7321 tggtcccgtc ggacgttgcc ggtcgagcgg aagaagttgg cgcggtagat cttcatgccg
     7381 ttggccgcgt ccgccttgaa cgtgtcggcg tagtgaagct gatgcgaagg tatcccgcgg
     7441 gcgatgacca accggcggat cgcgtcggcg agaaacgcac gcatggcgag cggcagcagc
     7501 agcggcaccg agaacacgcc catgtaggtc agccgcagcg cttggttgag cgcacgggca
     7561 aaccgtttcg gtcgatatgg ccgcagcagc gagttacgca gatacatcac aagatgatcg
     7621 gcgctagggc cggacaacga cgagaacgag gcgacgtggt cactagcacc cgcacgagac
     7681 aggtactccc acacccccgc cgatccccag tcatgagcca gcacatgcac gggctgaccc
     7741 ggcgcgaggg cgtcgatcac tgcggcgaag tcgttggcga aacagtgcat tgtgaaggcc
     7801 gaaacctttt tcggcacagt cgatttgccg gcaccgcggt tgtcgtaacg cagaatgcgg
     7861 aaccgatcgg cgagcagcgg caccacgcca ttccacaaga cgtgcgaatc cggccagccg
     7921 tgcacgagca ccaccgtcgg cccatcggga ttgccttctt cgtagacggc gatgcgcacc
     7981 ccgtccgtgc tgtcgatgaa ccgctcgctg atctcgccca tggcacctcc gcacatttag
     8041 tgggaccgct ggtaccgtat cagccctcca gctcctcacg aagggcgcgc tcacgttcaa
     8101 tccgcctgcg cgcgtcgtag tcccgcatcc gctgcggata acccaccttc tgcacgtcat
     8161 agaccggaat cttgagccgg tcgctcagcc gccgcgcgcc cgcatctccg ccggctctgc
     8221 ggcgggtcca ctcaccgtct gcggccacca ggacgaccgt gacatcggtg acggtggttc
     8281 tcggttcgac gaaggcctcc acgccggtgt gctcggcaac ccactgctgt aggtagcgca
     8341 ggtcggccgc ggggtcattg cctgcgctac cccgtgctcg gcggccaccc ctaaacctgt
     8401 cgaacagtcc cacctgggct cggctcctct ctcacctcta tcgatagtgc cagagctttc
     8461 agtccccagg tctgcgacga cctgcggtcg cagacgtgac aggatgggaa ccgacattcg
     8521 acacctgcgc ttgaacgttt cgagggagtc cacgcacatg gccatctccg tccagatgcc
     8581 cgcacttggt gagagcgtca cagaggggac cgtcacccgc tggctcaagc aagagggcga
     8641 cacggtcgag caagatgagc cgttactcga agtctccacc gacaaggtcg acaccgagat
     8701 tccctccccg gccgcgggtg tgttgaagaa gatcatcgcc caggaagacg acaccgttga
     8761 ggtgggcggt gagctcgcgg tgatcggcga cgccagcgag gacggcgaat catccggcgg
     8821 ccaagtcaag gcggccgaga agccggccga agaagaggag aagcccgccg aggagccggc
     8881 cgcacagccc gaaccggccg ccgaggagaa ggaagagcct gccgaagcac ccaagcccgc
     8941 cgcgcagtcg tccggcggtg gccaagccac gtcggtgaag atgcccgagc tgggcgagtc
     9001 ggtcaccgaa ggcacggtga cccgttggct gaagaaggtc ggcgactccg tcgaggtcga
     9061 cgagccgctg gtcgaggtgt ccaccgacaa ggtcgacacc gagatcccgt cgccggtggc
     9121 gggcacgctg gtgtccatca ccgccgagga ggacgacacg gtcgcggtgg gcggcgagct
     9181 ggccaagatc ggcgaagcag gtgcggaagc cgccgaagcg ccagcacccg agccgaagcc
     9241 ggagcccgag cccgagccca aagaggaggc caagcccgag ccgaagcccg aacccaaacc
     9301 cgagccgaaa ccggaaccca agccggagcc ggagccggcc caggaggcca agcccgaacc
     9361 caaaccggag cccaagcccc aaccggaacc gtcgccgacc tcagccgagg ccggtccgta
     9421 tgtgacaccg ttggtgcgaa agctggccgc cgagaacgac attgatctcg cgtcggtgaa
     9481 gggcaccggc gtcggtggcc ggattcgtaa gcaggacgtg cttgcggccg cggaagccaa
     9541 gaaggccccg gccgctccgg cgcgcgagga agcacccgca gccgcggcca agccggcggc
     9601 cgccgccgcg acgccagcac ccgcgctggc ccacctgcgt gggaccaccc agaaggccag
     9661 ccggattcgt cagatcaccg cgaagaagac gcgcgaatcc ctgcaggcca cagcgcaatt
     9721 gacgcaaacc cacgaggtcg acatgaccaa gatcgtggcg ctgcgcgcca aggcgaaaaa
     9781 cgggtttgcc gaacgggagg gcgtcaactt gacgtatctg ccgttcatcg cccgcgcggt
     9841 gatcgacgcg ctgaaactgc atccgaacgt caacgccagc tacaacgagg aaaccaagga
     9901 gatcacctac tacgacgccg agcacctcgg tttcgcggtc gacaccgaac aagggctgct
     9961 gtcgccggtg atccacaacg cgggcgatct gtcgcttggc gggcttgcgc gggcgatcgc
    10021 cgacatcgcg gcgcgggcac ggtcgggcaa cctcaagccc gatgagctgt ccggcgggac
    10081 gttcacgatc accaacatcg gtagccaggg cgcgcttttc gacaccccga tcctggtgcc
    10141 gccgcaggcg gcgatgctgg gcaccggcgc gatcgtgaaa cgaccacgag tgatcgtcga
    10201 cgagaacggc aacgaatcga tcggcatcag gtcggtcagc tacctgccac tgacctacga
    10261 ccaccggctg atcgacggcg ccgacgcggg gcgcttcctg accaccatca agcgccgcct
    10321 cgaagagggc gcgtttgagg ccgacctggg actgtagcca gccgtgacca acgccgtcat
    10381 cgcgatcgcg gggtcctcgg gtctgatcgg ctcggcgttg gtctacgcgc tgcgcgccac
    10441 cgaccgcaag gtgctgcgga tcgtgcggcg tgctccgtcg aacgcagacg aggtgttctg
    10501 gaacccagac accggcgagt tcgacccgag cacactggcc ggagtcgacg cggtggtcaa
    10561 tctgtgcggc atcaacgtcg gcgagaagcg gtggtcgggt gcgttcaaac aaagcctgcg
    10621 cgacagccgt atcggcccga ccgaagtgct ctcctcggcg gtggtcgagg ccgccgtccc
    10681 cacgctgatc aacgccagcg cggtcggcta ctacggtgac accaacagcc agactgtcga
    10741 cgaaaccgcc cctgcgggtg aaggtttcat ggcccagctc tgtgaggact gggaggccgc
    10801 cacctcgttc gctgcgcacg acggcacccg ggtcgtgctc gtgcgcaccg ggctggtgct
    10861 ggcgccggcc ggcgggattc tgggccggat caagccgctg ttctcgctcg gcctcggcgc
    10921 ccgactgggc aacgggcgcc agtacatgcc gtggatcagt ctcgaggacg aagtccgggc
    10981 gctgcttttc gccatctccc acgacgacgt gtcgggaccc gtcaacctga ccgggcctgc
    11041 gcccgtcacc aacgcagagt tcaccgcggc actggggcgc accgtgaacc ggccgacccc
    11101 attggtcgcg ccgggattcg cgctgcgggc cgttttcggc gagttcgccg aagaaggcct
    11161 gctcggcggg cagcgcgcga tccctgcggt gttggaacgc gcgggctttc acttccacca
    11221 caacaccatc ggcgaggcgc ttgccttcgc gaccgcgaag aaccatgaag ggtagcgttc
    11281 atgccgtggc ggcatccatc cgatcggcca cggcgccgat cgatattcgc cggctcggca
    11341 ccgtcgacta ccagacagct tggcagctgc agcgcgagct ggccgacgcc cgtatcgcag
    11401 gcggtcccga caccctgctg ctgctggagc atccgtcggt gtacaccgcg ggccgccgca
    11461 cgttgcccga ggaacgcccc gtcgacgaca ccccggtcgt ggataccgac cgcggcggca
    11521 agatcacctg gcacgggccg gggcagctcg tcggttatcc gatcgtcggt cttgccgaac
    11581 cgctcgatgt ggtgaatttt gttcggcgcc ttgaggaatc gataatcacg gcgtgcgcgg
    11641 ggttcggatt ggaaactgga cgtatcgagg gccgctcggg cgtgtgggta ccgtccgacg
    11701 gcgtccggcc ggctcgcaag gtcggcgcga tcggtatccg ggtgtcgcgc tcaaccacac
    11761 tgcacggctt cgccctcaac tgcgactgtg acctcagcgc gttctcgtcg atcgtgccgt
    11821 gtggcatctc cgacgccggc gtgacatcgc tgacggccga actgggtcgc cgggtgtccg
    11881 tggcggactt gctggggccg gtggccgacg ccgtgtgtga cgcgctcgac ggccgcctcg
    11941 tagtagcgtt gacgtagtga cagtcgctcc tgaaggcaga aaactcctgc gtctcgaggc
    12001 ccgcaatgcc gagacgccga tcgagcgtaa gccgccgtgg atcaagaccc gggccaagat
    12061 gggcccggag tacaaggagc tcaaggcgct ggtccgacgc gagggcctgc acacggtctg
    12121 cgaggaagcc ggctgcccga acatcttcga gtgctgggag gaccgcgagg ccaccttcct
    12181 gatcggcggc gagcagtgca cgcgtcgctg tgacttctgc cagatcgaca caggtaaacc
    12241 cgccgagctc gaccgcgacg agccgcgtcg ggttgccgag agcgtgcagg cgatggggct
    12301 gcgctactcc accgtcaccg gggtcgcccg cgacgatctg cccgacggcg gcgcgtggct
    12361 gtacgccgaa accgtgcggg ccatcaagaa gctcaacccc aacaccggcg tcgagttgct
    12421 gatcccggac ttcaacggcg agccgacgct gctcgagcag gttttcgagg cccgaccaga
    12481 agtgttggcg cacaacgtcg aaacggttcc gcgcatcttc aagcggatcc gccctgcatt
    12541 ccgctacgag cgcagcttga acgtactgac ggctgcgcgc gactacgggc tggtgaccaa
    12601 gtccaatctc atcctcggca tgggcgagac acccgacgag gtgcgcaccg cgctgcgcga
    12661 tctatacgac gccggctgcg acatcgtcac gatcacccag tacctgcggc cgtcggtgcg
    12721 ccaccatccg gtggagcgtt gggtcaagcc cgaggagttc gtcgagcacg aggagttcgc
    12781 caagggtctc ggcttcaccg gtgtgctggc cggcccgctg gttcgatcct cgtatcgggc
    12841 gggcaggctg tacgcgcagg cagtcgcgtc tcgacccgcc gtatcctgaa cacatggcga
    12901 aacagcgtga ccccgctgcg gcgaaggccg ccaaggccga ggcgaaggtg acccgcaagg
    12961 cggcgtccaa gcagcgccgc agccagttgt ggcaggcgtt ccagattcag cgcaaagagg
    13021 acaaacgcct cctgccctac atgatcgggg cgttcgtgct gatcgtgggc gcgtccacgg
    13081 cgttcgggat catctccggc ggcttcaccg gctacatgat gatcccgctt ggcatcgtgc
    13141 tcggcgcgct ggtggcgttc atcatcttcg gccgccgcgc acagaagtcg gtgtaccgaa
    13201 aggctgaagg gcagaccggc gccgcggcct gggcgctgga taacatgcgc ggtaagtggc
    13261 gggtgacacc cggcgtcgcc gcgaccggcc acttcgatgc ggtgcaccga gtgatcggcc
    13321 gaccaggcgt gatcttcgtc ggcgaaggcg cggcaaatcg cgtcaaaccc ttgctggccc
    13381 aggaaaagaa gcgcaccgcc cggttgatcg gcgacatccc gatctacgac atcatggtcg
    13441 gcaacgacga gggcgacgtg ccgctggcca agctcgaacg tcacctgaat aagctgcccg
    13501 ccaacatcac ggtcaagcag atggactcgc tggagtcgcg gctggccgcg ctgggcacca
    13561 aggccggacc ggccgcgatg ccgaagggcc cgctgccgac acaggccaag atgcgcaacg
    13621 tacaacgcac cgttcggcgc cgctgactac cgccgtacca ccgcggtatt cgtcactttg
    13681 tcctgaagtc cgcgccagtc gacgtcggtg atcagcgccg ggatgacgac ggcgatcaga
    13741 atcccgcgca ccacggcgcg gccgaatcca acgtgctgac gaccgtcgac aggcaccacc
    13801 atcagcccca acgcgaactg gccgggcgtg aatccgaaca gccgcaccga caccgcgccg
    13861 agcacgaacc agatgagcag gacggcggtg gacaggctgg tcatcgacac caagccgaac
    13921 gacatcgcaa gcgccgccag tccgtaggcg atcaaccagt cgacgaacag cgcggcgatc
    13981 cgtcggccca tcctggccaa ggatcgtggg cctgtccgcg gcaaaccgag ccgctggccc
    14041 gggtggtcat tgggtccttt gtcctggctg ccagagttcg gtccgggacc cgacagccag
    14101 gacccgattg cgcgggccat gcccccagga taagggcctt aagaagaggt ctagagtcgg
    14161 tcgcagttga gcgccgcagc cgccaaattg cgtaacttca gcgcaacatg cggttgacgg
    14221 ccgtgcaaca tcgtgtccat agcgtctacg cgcgagttac cagataaagg agagtactca
    14281 gtggcagaaa agaccgccga cgacatcctc aagcagatca aggacgacga agtcgagtac
    14341 gtcgacattc gcttctgcga tctgccgggc atcgtccagc acttctcggt cccggcttcc
    14401 gcgttcaccg aggacgtatt tgaggacggc ctcgctttcg acggttcgtc ggtgcgtggc
    14461 ttccagtcga tccacgagtc cgacatgctg ctgctgcccg acccggagac cgcgcgtatc
    14521 gacccgttcc gcgccgccaa gacgctgaac ctcaacttct tcgtgcacga tccgttcacc
    14581 cgcgaggcgt actcccgcga tccgcgcaac atcgctcgca aggcggagaa ctacctgatc
    14641 agcaccggca tcgccgacac cgcgtacttc ggcgccgagg ccgagttcta catcttcgac
    14701 tcggtgagct tcgactcgaa gatgaacggc acgttctacg agatcgactc cgaagccggc
    14761 tggtggaaca ccggcgagca gttcgagccc gacggcagcc ccaaccgcgg ctacaaggtg
    14821 cggcccaagg gcggctactt ccccgtcgca ccgtatgacc actacgtcga cctgcgcgac
    14881 cagatgtgca ccaacctgca gaacgccggc ttcaccctcg agcgcggtca ccacgaagtg
    14941 ggcaccgctg ggcaggccga gatcaattac aagttcaaca ccttgttgca tgcggcc
//
LOCUS       DASRJA010000449         5653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_596788,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000449 DASRJA010000000
VERSION     DASRJA010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596788"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2358)
                     /locus_tag="VFC01_19715"
     CDS             complement(<1..2358)
                     /locus_tag="VFC01_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81891.1"
                     /translation="MSMPSSSQQALTVLVAPHARTEEIFAVLADYSAVGLLAAFAWVD
                     AADVGGASTPATVLRDGRSEPAVLQQLLTAKRYQRIRVVVLVPAEAPSPERVPLVAEQ
                     TVEQTVRSTSMGARISLLRVLLTRGDAAAAAPDSTMVLEGWHNLLVAPEDSPGPGLGA
                     VPSGPITDPLEFAQRTAPVIAGVAGLWAGLEQTPFDTLEILPGQTVRAVRAFYRRLDS
                     SEVEERLRAQLFDPAGRLPLPRGGEVPVVYVEDVAAATQTMARGLWTKHRDLLRGPRL
                     SVSDVGAQAISIWAALKMFLKFMGAALRQAPGAWWSAVTGSVSSVLASTVQGTVFGGR
                     DSAFAVVTNTDVANWQHLGRSADTLNTALGGAPPAQHLAQRDLSALWMDFVTGALTLA
                     DGGRRAAGLEPVPVGSGVGVLAVASDVVPSAAESFSSIPTSLSAVIGVSSVEPADVLG
                     VADLRERLQRAYSDPAAGVEARTAGTELEGWQRFVSKSYAWQVGSILADFLHRARGEV
                     AQLVGEIQQAANRVSADERLQARQRAIGVILKTFFWSLVLVLVVLAGIAAAGWVRWPF
                     ALWTGGVMLALYILASLALFLFSQRDLFAETHLRQSQLGELETMQANLRTALQDVSRL
                     STAYGQLLSWCRVLGAVLRAPFGPAPVARPSPGRLFDGLPRSTQLGVADPDERQAADA
                     AHAIQRRLYALGWLTQPWQDMINEVAARLREEPEMLFRMPGIGTQSGLDQWSYAVASG
                     QVQPTGADALWGRVEQMFADGHGEVADALTGTVLVPGEDGHVPARQ"
     gene            complement(2361..>5653)
                     /locus_tag="VFC01_19720"
     CDS             complement(2361..>5653)
                     /locus_tag="VFC01_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HZN81892.1"
                     /translation="SQRLAAESALDTIATWAPRNPQEVSIPISAGAGQYRAIGRMITL
                     SKATEIHTRLQAAWDALFRVDTISQMSTVDVPGMGKFDPNEPPLVLVVSSMAGGAGAS
                     MALDVCRLLTLVSGLDPKLMGVFMVTPDIFDSLPQSSIVGVRANSLAMLGEIVASQAG
                     SAREHDVRLMRALGHQHGEGEPIPFARVFPVGRFIGADRTLFGDGSQWAVYRGLARGL
                     AGLMMSGAASDQFVSYDLGNTASPEGDRDLLGWGISAWDVLPWGTYGFASLSMGRDRY
                     AEYAAQRLARSCVDKLLFGHLQAGNPASSTEQLDSLLSSQWQAHCAELELPPNPGDEQ
                     TRTGILGRWIGSEAFAADSVAATVNGLIDRQLRGYLPNPEGMTAEQWVPIFRQALRNR
                     GSELLRACDDAAYAMAFSWHREFAGRVEQVVGGTIATMGLPYARELVDRLRRHIDDVL
                     APGVAQLGTMGPADIVAVPPQVDAALRSMRGVMTNADQVLATALDGFRAGVRRQLFAD
                     AAARIADVMRVVGAEMLRPLREALGEAMVLLEQARAEPPTDVGLARLTTDQYAAWPAD
                     ADELVPARFAEANNEVLLISSSAFKGRYELDLPKAVAGANAMVPLRTAIDDATTQVIL
                     GQWRTTGGVSAPGGLIERTATWVTRALGTDPETSRPRVPSMAQFDVHTRPGELLTRAR
                     LYVGRPGESFDEFCKVSLRDFVQGAGAAESELVMRRRDVTTKFAEALSLARPLASVND
                     EALQRVHPGQQTEYRYKFSEVPFAGQPVADALFDVLRTNPRIDQATKDNYHRSLSDED
                     SVTRVDIFGSYPNYSPLAFDSVLKPAAQQWARIAGPGRGTFWRYRRARPLPASLPMTD
                     DERRAMTAGWFLGQIVGRIQIPESPYTDAVRIYDADAGQWLNFPNPLLTPPSAFIATY
                     DWLPAVLESILLAIAQSHEPPVMHTLRPYGVLRELYDAHSQDPASGIVELSASEVLRE
                     FLVGSAAAPGVTPRIETIAAARSPEERAAAAQEWLTNVRDVAAQYLPGDMPGAAPGGV
                     FTSVPTRSTASKTPIFRDLAPDVFWATEVLIRLLQREAAALARGAATAGTGYLDDGPL
                     VIPEGGTF"
BASE COUNT          932 a         2033 c         1861 g          827 t
ORIGIN      
        1 ttgccttgca ggcacatggc cgtcctcacc gggtaccagg accgtgccgg tgagcgcgtc
       61 ggcgacttcg ccgtgcccgt cggcgaacat ctgctcgact cgcccccaca gcgcgtcggc
      121 gccggtgggc tgaacttgcc ccgaggcgac cgcatacgac cactgatcca gcccggattg
      181 cgtgccgatg cccggcatcc ggaacagcat ctcgggttcc tcacgtagcc gtgcggccac
      241 ctcgttgatc atgtcctgcc acggctgggt cagccagccc aatgcgtaca ggcgccgctg
      301 aatcgcatgc gcggcgtcgg cggcctgccg ctcgtcgggg tcggcgacac cgagttgggt
      361 cgagcggggc aggccgtcga agagccggcc gggtgagggc cgcgccaccg gcgcgggccc
      421 gaacggcgcc cgaagcaccg cccccagcac ccgacaccac gacagcagct gtccgtacgc
      481 ggtggacaga cggctgacgt cctgcagcgc cgtacgcaga ttggcctgca tcgtctccag
      541 ctcacccagc tgagattgtc gtaagtgcgt ctccgcgaac agatctcgct gcgagaacag
      601 gaacaacgcc agcgacgcca gaatgtagag ggcgagcatc acaccgcccg tccacagcgc
      661 gaacggccag cggacccagc ccgcggcggc gatcccggcg agaacgacga gcaccaaaac
      721 cagtgaccag aagaacgttt tcaggatcac gccgatcgct cgctggcgcg cctgcagtcg
      781 ctcgtcggcc gacacccggt tggcggcctg ctggatctcg cccaccagtt gggcgacctc
      841 gccgcgggcg cggtggagaa agtccgccag gatggaaccg acctgccagg catagctttt
      901 cgacacgaac cgttgccagc cttctaactc tgtgcccgca gtacgtgcct cgaccccggc
      961 cgcgggatcg gaataggcgc gttgcagccg ctcccgtagg tccgcaacgc cgagcacatc
     1021 ggccggttcc accgagctga ccccgatgac tgcggacagc gacgtgggaa tcgacgaaaa
     1081 cgattcagcg gcactgggaa ccacgtcgga ggcgacggcg agcaccccga ccccggaacc
     1141 taccggcacc ggctccaacc ccgcggcgcg acggcccccg tcggccaggg tcagcgctcc
     1201 ggtgacaaaa tccatccaca gcgcgctgag gtcccgctgt gcaaggtgtt gggccggcgg
     1261 cgccccgccc agcgcggtgt tcagcgtgtc ggcgcttcga cccaggtgct gccagttggc
     1321 cacatcagtg ttggtgacca ccgcgaaagc ggaatccctg ccgccgaaca ccgtgccctg
     1381 caccgtgctc gccagtaccg atgacaccga acccgtcacc gccgaccacc acgcccccgg
     1441 cgcttgccgc agcgcggcgc ccatgaactt caagaacatc ttcaatgctg cccagattga
     1501 aatagcttgg gcgccaacgt cactcacaga cagtcgcggc ccgcggagca ggtctcggtg
     1561 ctttgtccac aaccccctgg ccatggtctg tgtggcagcc gcaacatcct ccacgtagac
     1621 gacgggcacc tcaccgccgc ggggcagcgg aaggcgtccc gcggggtcaa acagctgggc
     1681 gcgcaggcgc tcttcgacct cgctgctgtc cagccggcgg tagaacgccc gcaccgcgcg
     1741 caccgtctgc ccgggcagga tctccaacgt gtcgaacggt gtctgctcca atcctgccca
     1801 cagtccggcg accccggcga tcaccggtgc ggtgcgttgc gcgaattcga gcggatcggt
     1861 gatcgggccg ctcgggactg cacccaggcc gggtcccggc gaatcctccg gggcgacaag
     1921 caggttgtgc catccctcca gcaccatcgt cgaatccggt gccgccgctg cagcgtcccc
     1981 ccgggtcagc aggacacgca gcaggctgat gcgcgcaccc atcgaagtgc tgcgcaccgt
     2041 ctgctcgacc gtctgctcgg ccaccagggg cacccgctcg ggggacggcg cctcggcggg
     2101 caccaagacc accacccgga tccgctggta gcgcttggcg gtgagcagct gctgcaacac
     2161 cgccggctcg gagcgaccgt cgcgcagcac ggtggcaggg gtggacgcgc caccgacgtc
     2221 ggcggcgtcg acccacgcga acgcggcgag cagtccgaca gcggaatagt cggccaggac
     2281 ggcgaaaatc tcctctgtcc gggcgtgcgg agcgaccaac accgtcagcg cctgctgcga
     2341 actgctcggc atcgacatct tcagaacgtg ccgccctccg ggatcaccag gggcccgtca
     2401 tcgagatagc cggtcccggc ggtcgccgcg ccccgcgcca gcgccgcggc ctcgcgctgc
     2461 aacaaccgga taaggacttc cgtggcccag aacacgtcag gcgcgagatc gcggaagatc
     2521 ggcgtcttcg acgccgtcga ccgtgtcggg accgaggtga acactccgcc gggcgccgca
     2581 ccgggcatgt cccccggcag atactgcgcg gcgacgtcgc gcacattcgt cagccattcc
     2641 tgggcggcgg cggcgcgctc ctcgggactt cgggccgcgg cgatcgtctc gatccgcggc
     2701 gtcacaccgg gcgcagcagc gctgccgaca aggaactcgc gcagcacttc gctggccgac
     2761 aactcgacga tgccactggc cgggtcctgc gaatgtgcgt cgtagagttc tcgcagcacg
     2821 ccgtagggcc gcagcgtgtg catcaccggc ggctcgtggg attgagcgat cgcgagcagg
     2881 atgctctcca gcactgcggg cagccagtca taggtggcga tgaacgccga cggcggtgtc
     2941 agcagcgggt tggggaagtt caaccattgg cccgcatcgg cgtcatagat gcgcaccgcg
     3001 tcggtgtacg gcgactccgg gatctgaatc cggccgacga tctgacccag gaaccagccc
     3061 gcagtcatgg cgcggcgctc gtcgtcggtc atcggcagtg acgccggcaa gggtcgcgcg
     3121 cgacgatagc gccagaacgt tccgcggccc ggcccggcga tccgggccca ctgctgtgca
     3181 gcgggcttga gcaccgagtc gaacgccagc ggcgagtagt tggggtagga cccgaagatg
     3241 tccacccgtg tcacgctgtc ctcatccgat agcgaccggt ggtagttgtc cttggtggcc
     3301 tggtcaatac gcgggttggt gcgcagcacg tcaaacaacg cgtcggcgac gggctggccg
     3361 gcgaagggca cctcggagaa tttgtagcgg tactccgtct gctgcccggg atgcacccgt
     3421 tgcagcgctt cgtcattcac gctggccagc gggcgggcca gcgacagcgc ctcagcgaac
     3481 ttggtggtga cgtctcggcg acgcatgacc aattccgatt ccgccgcgcc agcgccctgc
     3541 acgaagtcgc gcagcgacac cttgcagaac tcgtcgaacg attcccctgg ccggcccaca
     3601 tacagccggg cccgcgtcag cagctcgcct gggcgggtgt gcacatcgaa ctgcgccatc
     3661 gacggcaccc ggggccgact cgtctcgggg tcggtgccca aggcgcgcgt cacccatgtt
     3721 gcggtgcgtt cgatgagacc tccgggtgcg gacacgccgc cggtggtgcg ccactgtccc
     3781 aggatcactt gtgtcgtcgc gtcgtcgatt gcggtgcgca gcggcaccat tgcgttggca
     3841 cctgccacgg ccttgggcag atcgagctcg tagcggccct tgaaggcgga cgagctgatc
     3901 agcagcactt cgttgttggc ctcggcgaac cgcgcgggca ccagctcgtc ggcatcggca
     3961 ggccaggccg cgtactgatc ggtggtcagc cgcgcaagcc cgacatcggt gggcggttcc
     4021 gcgcgcgcct gctccagcag caccatggct tcgccaagcg cctcgcgcag tggccgcaac
     4081 atttccgcgc ccaccacccg catcacatcg gcgatccgcg cggcggcgtc ggcgaacagt
     4141 tggcgtcgca cgcctgcgcg gaacccgtcc agcgcagtcg ccaggacctg atcggcgttg
     4201 gtcatcaccc cgcgcatcga gcgcagcgcc gcatccactt gcggcggcac cgcgacgatg
     4261 tccgccggcc ccatcgtccc gagttgcgcc accccggggg ccagcacgtc gtcgatgtgg
     4321 cggcgcaacc ggtcgaccag ttcccgggca tacggcaggc ccatcgtcgc gatcgtcccg
     4381 cccaccactt gctcgacccg cccggcgaac tcccggtgcc agctgaacgc cattgcgtag
     4441 gcggcgtcgt cgcaggcccg cagaagttcg ctgccccggt tgcgaagagc ctgacggaag
     4501 atcggaaccc actgctctgc ggtcatgccc tccgggttcg gtagataccc tcgcagctgc
     4561 cggtcgatga gcccgttgac ggtggcggcc accgaatcgg cggcgaaggc ctcggagccg
     4621 atccagcggc cgagaatgcc cgtgcgggtc tgctcgtcgc cggggttcgg cggcagttcc
     4681 agctcggcgc aatgcgcctg ccactggctg ctgaggagcg aatcgagttg ctcggtgctc
     4741 gacgccgggt tgccggcctg cagatgtccg aacagcagct tgtccacgca gctgcgtgcc
     4801 agccgttgcg cggcgtactc ggcgtagcgg tcgcgaccca tgctcaagct ggcgaaaccg
     4861 taggtgcccc aaggcaatac gtcccacgcg gagatgcccc agcccagcag gtcgcggtcg
     4921 ccctccggcg acgccgtgtt gcccaggtcg taggagacga actggtcact ggccgcgccg
     4981 ctcatcatca gtcccgccag gccgcgtgcc agtccccggt agacggccca ctgcgaacca
     5041 tctccgaaca gcgtgcggtc cgcgccgatg aagcggccca ccgggaacac ccgcgcgaac
     5101 ggtatcggct ccccttcgcc gtgctggtgg cccagcgccc gcatcagccg cacatcgtgt
     5161 tcgcgcgccg agcccgcctg actggccacg atctccccaa gcatggccag cgaattggcg
     5221 cgcaccccga cgatggagct ctgcggaagc gaatcgaaga tgtcaggcgt caccatgaag
     5281 acgcccatca gtttcgggtc aagacccgac accagggtca gcagccggca cacatccagc
     5341 gccatcgacg cccccgcgcc gccggccatc gacgacacca ccaggaccag cggcggctcg
     5401 ttggggtcga atttccccat gcccggcacg tcgacggtgg acatctgcga gatcgtgtcc
     5461 acccggaaaa gcgcatccca cgcggcctgc aggcgggtgt ggatctcggt ggccttgctc
     5521 agcgtgatca tccgcccgat ggcccggtac tggccggcgc ctgccgagat cgggatggac
     5581 acctcctgtg ggttgcgtgg cgcccaggtg gcgatggtgt ccaaggccga ttcggcggcc
     5641 agccgctgcg aca
//
LOCUS       DASRJA010000450        32509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2386075,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000450 DASRJA010000000
VERSION     DASRJA010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 32509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32509
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2386075"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFC01_19725"
     CDS             <1..571
                     /locus_tag="VFC01_19725"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZN81893.1"
                     /translation="NHTWEHPNMTAIPPEDIAGQISKANAAIQAATGQRPNLLRTAGG
                     LINDNVLAAAKQQGMADINWDVIPFDWINDSNTAATTYMLKTQIKPGSVVLFHDTYSS
                     TVDVVYQFIPVLKSNGYHLVTVSQLLGPREPGSSYGSRDNGPPANDITDIPPAEIPTL
                     PNTPSPKPMPNFPITDIEGANSGGANNGA"
     gene            592..1812
                     /locus_tag="VFC01_19730"
     CDS             592..1812
                     /locus_tag="VFC01_19730"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZN81894.1"
                     /translation="MRTDTAFVLTVLVLCYAVVSGLVKRWYIAPALIFVGFGMALGPF
                     GFGVIEAGADTASFTVLAQLALTVILFNQAAMLDLPSVVRRGHITFRLLVIGMPLAIV
                     LGTVVALLVLPQMPLWEAVCVAAIVAPTEVALIDALLEDRRIPERVRHALSVESGCYD
                     GVALAAMLAALALASEQNDPDPARWGWFAVRTEVVSLAVGLGIGVVGGLVIVRSRQRG
                     WMSDTWAQLATVALALVCFEFGERVHGSGFVTAFAGGLAYSMMVRRAGAQMPSQVTDA
                     AGQLLELMVFAMFGSYAVIVGWRDADWRVVLFAVVALILVRLVAVSTALLGTDLPARS
                     RLFIGWFGPRGIGTLVLGLLMIQRGEIEQSAVITQAVVVTVTVSLVVHSFTAPLGVRL
                     WPAPSPEKTTKANG"
     gene            complement(1809..2018)
                     /locus_tag="VFC01_19735"
     CDS             complement(1809..2018)
                     /locus_tag="VFC01_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81895.1"
                     /translation="MTVTLDPATRNPATLVTTGTSQPALRTGFALLGLVSLLDLPRAD
                     RADRVVALSGAAAAGWFLVRGGRRP"
     gene            complement(2118..4871)
                     /locus_tag="VFC01_19740"
     CDS             complement(2118..4871)
                     /locus_tag="VFC01_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN81896.1"
                     /translation="MSGVGVQSRQTESRRIAGFLASAATEPSALLIEGEPGIGKTTLW
                     LEALELARNQGFGVLSARAAAAESVLAYTALADLLDGVPASAWADLPDPQRFAVDQVL
                     MRADNGSATDQRAVAAAFLSVVEYLTDEGPLLVAIDDLQWIDPSSMHVVAFAARRLTG
                     HVGILGSVRTDGDGIGGAWVQLPRPDAVSRIRLNPLGIHDLHAAVSTRLRRPFSRPTM
                     GRIHEVSGGNPFYAIELARAIAERAPGVETSLPRTLADVVRTRINGLDPDVHDALLAA
                     SCMASPTVELVSSATVSDDDRLVELLENAESKGIIAIDGNRIYFAHPLLARGVYTEST
                     PGHRRTMHRRLADIVDEPELRARHLALAATSGDEVTLRALDTAAESARVRGAPAAAAE
                     LMDLAIGLGGDTPQRRLRSALHHFDAGDQERARAVLEETIERLAPGDLRAEALSRLAI
                     VRLYGDGFYEGARLLQQALSEVHADSPLRVQTLITLAYALLQANQAQASVTMADQAVA
                     KAERLGQPHLLGLALGMRVILRFLSGKGFAEADLRRAIELEDPNIFTPLVFRPSVQRA
                     LLLEWTGRPDEAHGVLEGVRRRCADTGEEGELIFIAQHVVLSSIWRGDFVEAGLVAED
                     AMERARQLGGDTPLFIAHSLRAKLAIYAGREADAREAAEAALAFGRRTGAFRLMDRVV
                     AALGFLEVSLGRYEAAVATLQPLVTKFDPASTPTELPSAAFLTDAIEALIRVDRGAEA
                     EPLIDALERNGEQQNRAWMIAVGARGRSMLLAARGDLDAASAAATRALAQHDRLPMPF
                     ERARTLLLLGQLQRRQRRKESASASLQDALDIFERLHVRLWADRARAELARANVGPRQ
                     SSQLTPSEQRVAELAASGMKNRDVATTLFISPKTVEANLARIYRKLGIKSRAELGRHM
                     GKT"
     gene            complement(4972..7722)
                     /locus_tag="VFC01_19745"
     CDS             complement(4972..7722)
                     /locus_tag="VFC01_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN81897.1"
                     /translation="MTAGLASRPSEDGALEEFLDSACSAPSGLLVEGEAGIGKTTLWL
                     AGLERARDRGFQVLSARAAGAESVLAYTALADLLTGIDQDTWADLPDPQRMAIDRVHS
                     RDQSTDVVTDQRAVAAAFLSVVERLTNDGPALLTIDDLQWLDPSSAHVIGFAARRLSG
                     PVGFLATVRTEPNNSDAISWLQLPRPDAIQRITVRPLTQPALHTVVKEAVGRTLPRAA
                     MARIHQISAGNPFYAIELARAALTDNAGDVLPGTLTELVRARVGSLDPAVQDLLLAAA
                     CVATPTVELVAEAANTDHDRMIELLDAAERHGVISIQGNRIRFTHPLLAGSVYTGASP
                     TQRRLMHRRLAGVVDEVELRARHLALASTTGDPETLDALDDAAASAHARGAPAAAAEL
                     LELAIALGGDIPLRRVRLAGHVFDGGDLARARTLLQRAIAELQPGPVRAEALHSLAVV
                     IFNDDGYPEASQVLQRALAEDRPGQALRIRMLTALAHALYNTGEPDTAWQCAEQAVTL
                     AEQLGVPGLLSQALGARSMLLFLRGDGVDEPRLQRALELEDHESFTPTVLKPSVEHAL
                     LVGWTGDLDTSHQRMQAIQRRCTERGEEGDLIFIDFQVVLNRIWRGDFAEASRVTEAA
                     TELASQLGGDFPAMLTLVLRAWLAVFSGHEHDARPAVADAIDASKRSGTGWHDEWSLT
                     ALGFLETSLGNYDAALSALEPLLSRSGLSPNLTEIVMASFIPDAVEALTAVGRANEAV
                     PLVDALERNGRRLDRAWMLAVGARGRAMVQAARGDVEAAVQSARRALTEHDRLPMPFE
                     RARTQLLLGQLIRRDRSEATAVLRDALATFEELGTQLWADRARAQLAGTRSRDHTDVS
                     LTTAEQRVAELAASGMTNRDVAAKLFISSKTVEATLARVYRKLGIHSRAQLGRYMNTI
                     RR"
     gene            complement(7921..9345)
                     /locus_tag="VFC01_19750"
     CDS             complement(7921..9345)
                     /locus_tag="VFC01_19750"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HZN81898.1"
                     /translation="MSVASSTSQNVEYRIEHDTMGEVRVPITALWRAQTQRAVENFPI
                     SGRGLERTQIRALGLLKGACAQVNKDLGLLAPEKADAIIAAAGEIADGLHDDQFPIDV
                     FQTGSGTSSNMNTNEVIAGIAAQNGVTVHPNDDVNMSQSSNDTFPTATHIAATEAAVR
                     HLIPALEVLHESLAGKARQWRTVVKSGRTHLMDAVPVTLGQEFSGYARQVEAGIERVK
                     ATLPRLGELAIGGTAVGTGLNAPDDFDERVVAVLTEQTGIAELRVAANHFEAQAARDG
                     LVEASGALRTIAVSLTKIANDIRWMGSGPLTGLGEIQLPDLQPGSSIMPGKVNPVIPE
                     AVTQVAAQVVGNDAAIAWGGGNGAFELNVYIPMMARNLLESFKILTNASKLFAERCID
                     GLVANEERLRELAESSPSIVTPLNSAIGYEEAAAVAKQALKEKKTIRQTVIDRGLIGD
                     KLSLEELDKRLDVLAMAKVKDGDA"
     gene            complement(9370..10398)
                     /gene="glpX"
                     /locus_tag="VFC01_19755"
     CDS             complement(9370..10398)
                     /gene="glpX"
                     /locus_tag="VFC01_19755"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HZN81899.1"
                     /translation="MSPARGEAPDRNLALELVRVTEAGAMAAGRWVGRGDKEGGDGAA
                     VDAMRELVNSVSMRGVVVIGEGEKDNAPMLYNGEDVGNGDGPECDFAVDPIDGTTLMA
                     KGLANAISVLAVSERGTMYDPSAVYYMDKIAVGPDAADVIDITAPIGENIRRVAKAKN
                     VSVSDLTVCILDRPRHEQLIADVRDTGARCRLISDGDVAGAISACRPNTSTDMLAGIG
                     GTPEGIIAAAAIRCMGGAIQAKLWPKDDEERQKAIDRGYDLDQILTTEELVSGDNVFF
                     SATGVTDGDLLNGVHFYGGGCTTQSIVMRSKSGTVRMIEAYHRLSKLNEYSAIDFTGD
                     SNAYQPLP"
     gene            10453..11100
                     /locus_tag="VFC01_19760"
     CDS             10453..11100
                     /locus_tag="VFC01_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4245 domain-containing protein"
                     /protein_id="HZN81900.1"
                     /translation="MTTEPLPAPKPQKPRILQDGRDMFWSLAPLVIACIVLAGMLGMC
                     SFQPNGPGKGPAPSYDAPAALQADADALKIPIRLPRLPDGWQANSGSRKGIDGGRTDP
                     ASRQPARAVSSTVGYLAPTGMYVSLTQSNADEDKLVESINTNLYPTGTQEVDGVKWVV
                     YEGGGQDGKPAEPLWTTRLNGPTGPAQIAISGAAGTDEYRTLAQATQTQSPLPAK"
     gene            11109..11906
                     /locus_tag="VFC01_19765"
     CDS             11109..11906
                     /locus_tag="VFC01_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZN81901.1"
                     /translation="MTAPAADLTGWTVAPFSAAGYTHDVYRKGEGPGVVLIPELPGMS
                     PEVIGLGNHLVDNGFTVACPSLFGTPGAAAVRPGAIPVMVRACISREFAGFALNADRP
                     VAHYLRALARDLNEKTPGKGVGVIGQCFTGGFALAAAVDDSVLAPVLSQPSTPISLTP
                     KMKRDPGLSEGELKVIEQRAANEGLCALGLRFSEDPLAPGERFKTLKARLGDAFEVIE
                     IDSKKGNEHSFGKRTHSVLTLEVRERDGHPAYEARKRVVQFLKERLT"
     gene            complement(11887..13062)
                     /locus_tag="VFC01_19770"
     CDS             complement(11887..13062)
                     /locus_tag="VFC01_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZN81902.1"
                     /translation="MDTEFTLTQKRALAVATVIAILFGAYFLRGYFILIVVAAVAAYL
                     FTPLFNRLNKRFGTGLSATLTLLAAIAAVVVPMGLFVMLAVVQITNMVERVADWVGRT
                     DLSALGDQALRFVNDVLHRVPFINVTVTPDSLRGSMATAAQKVGEWLLGLLQGAAGSV
                     FGGVTAAILFLYVFLSLLTNRDKVLLLIRRLNPLGEEITDLYMSKMGAMVKGTVMGQF
                     VIAVCQGVAGATSIYIAGFHQGFFLFAVLLSALSVIPLGSGIVTIPFGIGMILFGNVF
                     GGVFVILFHLIVVTNIDNFLRPILVPRAARLDSALMLLAVFAGIAMFGAWGIVIGPVL
                     MIVIVTTISVYLAVYKDVPMEEPDDDEKPKQRRLLGWWARRSGKNKPAKAPAKSTSP"
     gene            13183..13434
                     /locus_tag="VFC01_19775"
     CDS             13183..13434
                     /locus_tag="VFC01_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81903.1"
                     /translation="MPAHLGVRSLCSRIAGCSKYQRRVVAGQAACRQLSEWINDDRGA
                     QIVAWEFVLDHLRQIVWVGSTDAAATVVWAFTGRTAPVL"
     gene            13565..14740
                     /locus_tag="VFC01_19780"
     CDS             13565..14740
                     /locus_tag="VFC01_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN81904.1"
                     /translation="MRRAYVFRLRPTARQHVVLAACAESHRELYNAALQERRDAWAHK
                     TRISYGDQSAQLTEMRSVRPDQAVWSFSSQQATLRRLDKAFAGFFRRVKRGQKAGYPR
                     FKATARFDSVEWPKDGDGARWLPERRRVYLKGVGEVKVNLHRQVEGQVKTIQIKRQGR
                     HWLLVLSCDDVPTRPLPATGRQAGVDVGIVSFATMSDGERVANPRWGRVAGRGLASAQ
                     QRLARANRGSNNRERKRETVAARHRKISNQRKDFHHKQARALVERYDLLVVEDLQIVN
                     MVRRAKPVPDPDNPGQYLANGARAKTGLSRSFSDAGWGRFVSILRAKAEEAGRIWIEV
                     DPRHTSDRCEHCGHADSRNRVTQAVFECQRCSHRAHADEHAARNLLRAGLALHAQAA"
     gene            complement(14798..15628)
                     /locus_tag="VFC01_19785"
     CDS             complement(14798..15628)
                     /locus_tag="VFC01_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN81905.1"
                     /translation="MSFADKQAIVTGAGSGIGAALCRALVSAGATVVCTDIDGDAAER
                     TAAALGTTARSAQLDVTDAAAVQQAVDEVVDRAGPLDLMFNNAGIVLGGDTELLTLEQ
                     WNAIIDVNIRGVVHGVAAAYPLMVRQGHGHIINTASMAGLTAAGQITSYVTTKHAVVG
                     LSLALRSEAISRGVGVLVVCPSAVETPILDKGAIGGFVGRNYFLEGQGVKTAYDPDRL
                     ARDTLRAIEKNKAILVKPRLAHAQWLFARLSPTLMQRMSIGFIEKQRASQATGRSQLS
                     "
     gene            complement(15625..16398)
                     /locus_tag="VFC01_19790"
     CDS             complement(15625..16398)
                     /locus_tag="VFC01_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN81906.1"
                     /translation="MSNGNQRSAVVVGGASGIGWATAQALAAQGDQVTVADRNADGAQ
                     ARAGELGGPHTAAAVNVTDENSVQRLFEQTGPVDVVVNTAGFSNIGLITDMPVDQFRD
                     VVDVCLTGAFIVAKHAGRSLRTGGVLVSISSLNARQPAAGMSAYCAAKAGLSMLTQVA
                     ALELGPRGIRVNAVAPGFVHTPLTEPAAAVPGVVEEYLENTALGRAGRPEDVANAVLY
                     LCSPGASWLTGEVLDLNGGAHLKRYPDILGHVMKLAEAQ"
     gene            16463..16924
                     /locus_tag="VFC01_19795"
     CDS             16463..16924
                     /locus_tag="VFC01_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN81907.1"
                     /translation="MTDLKLSDSISVAVTPDELYALVSDVTRTGEWSPVCRACWWDDG
                     DGPRVGAWFTGRNELPERTWETRSQVVAAEPGREFAWEVNNGWVRWGYTLEPEEGGTR
                     LTESWEFLPAGVAGFRERYGEHANAHIEHRTNMARTGIPATLAAIKKAAEA"
     gene            17145..18107
                     /locus_tag="VFC01_19800"
     CDS             17145..18107
                     /locus_tag="VFC01_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HZN81908.1"
                     /translation="MADDAQRRIARFIEPFRVDPGSKVNLAKDFDPAFEAGIKTKDDG
                     VELLKDGVELLAQYQARLAAQDTQGVLVVLQALDAAGKDGTIRHVMTGVNPQGVRVES
                     FKQPSSRELDQDFLRRYQERLPSRGEIGIFNRSHYEEVLVVRVHPEILRLQKLPAETL
                     EHHVWKRRYEAINDWERYLSENGFRIVKLFLNLSKEEQRQRFLRRIDLPDHNWKFSLH
                     DVEERQYWDAYQNAFSEMFSHTSTEWAPWYVIPADRKWFARTAAAAVIANALIDIDPQ
                     YPTLSEDALHDLSARKVALESEAPSVAAPTANGAAARTPIKKGA"
     gene            18111..19649
                     /gene="glpK"
                     /locus_tag="VFC01_19805"
     CDS             18111..19649
                     /gene="glpK"
                     /locus_tag="VFC01_19805"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HZN81909.1"
                     /translation="MTAKFVAAIDHGTTSTRCMLFDAKGQPVATGQRAQTMYYPHPGW
                     VELDMDEVWRGTQECIHDALASVGASSADVAGIGITNERETVVLWDRRTGHPVARSIT
                     WQDTRTADAADALAADGGIHRFQHQTGLPISTYSSALKLAWLLDEDPARREAARRGDL
                     LFGTPDSWLLWNLTGGPDGGLHATDPTNASRTLLMNLKTLEWDEDLLDLMEIPRQVLP
                     EIRSSSEIYGMAVGDLAGVPVAGLIGDQQASLFGHTCFDHGDMKNTYGTGAFMLFTLG
                     NEPVMSEQGLVTTVAWKLGNADPVYALEGSVAVAGALVQWLRDNLGIIHDASEIESLA
                     RSVDGSEGVVLVPAFSGLFAPYWRDDARGVLVGLTRFSTKGHIARAALEATAYQTYDL
                     AQAMLTDTGLAQLGELHVDGGMTQNDLLMQFQADILGSTVAAPASTQMTATGAAYAAG
                     LATKFWSDLDELRANYAITRRWRPQMGDDERARGVAAWHDGVERTLHLAERQPVGPAR
                     PASA"
     gene            complement(19639..20517)
                     /locus_tag="VFC01_19810"
     CDS             complement(19639..20517)
                     /locus_tag="VFC01_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HZN81910.1"
                     /translation="MTNELVRVPPQTAVLGSEHHYAEEGPARPVSVDGFWIQTHQVTN
                     TEFAEFVTATGYLTVAERPLNPADYPDAPAENLQPGSMVFTRTSGPVDLRHLNLWWTW
                     TPGASWRHPVGPHSSIDKRAEHPVVHVAYEDAEAYAAWAGLSLPTEAEWETAARGGLN
                     GAAYTWGDEPEQPGEPLANYWHGDFPWRPDRGYGRTTDVGSFPPNGYGLVDMAGNVWE
                     WTTDWYADTRAGDPCCEADSYDPRQPQFRVPRKVVKGGSFLCADNYCLRYRPAARRPQ
                     PVDTGMSHIGFRCVKR"
     gene            complement(20510..21412)
                     /locus_tag="VFC01_19815"
     CDS             complement(20510..21412)
                     /locus_tag="VFC01_19815"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZN81911.1"
                     /translation="MAELASWVDGPTKSAILDFVGRVTSEGGPDYVEPQARVAVFDND
                     GTLWCEKPMYIQLDFLLRRFKEQAEADPSLRDQQPYKAAYTGDLQWLGDAVSKHYQGD
                     DTDLKPLMGAILTAHHEITVEEHAGRINTFFAEAKHPTLGRAYTSCGYAPMVELLRYL
                     ETNGFICYIVSGGGRDFMRPVTGPMYGVPPERVVGSSVGLVYRDGHLYTTDQPEFLDD
                     GPMKPVRLWSRIGRRPILAVGNSNGDIEMLEFTRGMRLLVLHDDKDREFDYVAGAEKS
                     LEQAAKDDWTVVSMRNDWTTVFSD"
     gene            complement(21415..22959)
                     /locus_tag="VFC01_19820"
     CDS             complement(21415..22959)
                     /locus_tag="VFC01_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZN81912.1"
                     /translation="MPDKKPNILVIWGDDIGISNLSCYSRGMMGYFTPNIDRIADEGM
                     LFTDSYGEQSCTAGRSSFITGQSVYRTGMSKVGMPGVDVGLQKEDPTIAELLKPLGYA
                     TGQFGKNHLGDLNKYLPTAHGFDEFFGNLYHLNAEEEPEHEDYPTAEEAPLLRKALLP
                     RGVIHSWATDEDTGEVDDRYGPVGKQRIEDTGPLTKKRMETVDDETTSACIDFIRRQH
                     EAETPFFVWMNMTHMHFRTHTKPESKGQAGRWQSPYHDTMIDHDRNVGQLLDLVDELG
                     IAEDTIVIYSTDNGPHANSWPDGATTPFRSEKATNWEGAFRIPEMIRWTGKIKPRSVS
                     NEIVQHHDWLPTFLAAAGEPDIVEKLKKGHEAGDMKYKVHIDGYNLLPYLTGEVEKSP
                     RRGMIYFSDDCDVLGIRAENWKIVFMEQRCPGTLQVWFEPFTPLRAPKLFNLRTDPYE
                     HADVTSNTYWDWLIDRLYLMFYGSAIVTQFLQTFKEFPPRQEPASFTINHAVEELNKF
                     LASRGG"
     gene            complement(22963..24504)
                     /locus_tag="VFC01_19825"
     CDS             complement(22963..24504)
                     /locus_tag="VFC01_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZN81913.1"
                     /translation="MPNGKPNILVIWGDDIGISNLSCYSDGLMGYRTPNIDRIANEGM
                     RFTDSYGEQSCTAGRAAFISGQSVYRTGMSKVGIPGADVGWAAEDPTIAELLKPLGYA
                     TGQFGKNHFGDMNKYLPTVHGFDEFFGNLYHLNAEEEPEQFDYPHKDQFPLLYELAHP
                     RGVLKCKATDEVSSEADDPKYGPVGKQTIEDTGPLTTKRMETIDDDIADATIDYIKRQ
                     HAAGNPFFVWCNFTHMHLYTHVKPESRGQAGLWQSEYHDAMIDHDKNVGTVLDVLDEL
                     GIADDTIVIYSTDNGPHRNSWPDAGTTPFRSEKNTNWEGAFRVPELIRWPGKIKAGTV
                     SNDIIQHHDWLPTLLAAAGDPDVMDKLKKGYQIGDVEYKVHIDGFNLLPYLMAEVEHS
                     PRRGFFYFSDDGDLVAMRFENWKVVFAEQRCQGTLRIWAEPFTPLRVPKLFNLRTDPY
                     EYADITSNTYYDWFMHRDYFVFYATAMATKFLETFKEFPPRHPPASFSIDQAVEKLHE
                     FLAKD"
     gene            24650..24928
                     /locus_tag="VFC01_19830"
     CDS             24650..24928
                     /locus_tag="VFC01_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1876 domain-containing protein"
                     /protein_id="HZN81914.1"
                     /translation="MYDKDLSNKWRVEIDFDEDETHTHATVRAQCGDAETVTALGDAY
                     RNPKDSSQPMVGEEIAAARGLIALGTELLQRASSQIEQATQQPVHLYR"
     gene            24932..25456
                     /locus_tag="VFC01_19835"
     CDS             24932..25456
                     /locus_tag="VFC01_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HZN81915.1"
                     /translation="MALAVCLLFDPKSERAVRALWDRVEEQGVGSLRSHTHGSHVPHV
                     SYAVLRKWDQEAVTDALAGLDPGEPVNLSFDGVGLFRRGRTWLVAGVTADFVARQQRV
                     VEAVTATGAELHKHYRPGIWLPHCSLAPRATLAQLPQLVAAVMDVLPLPARLDRAALV
                     NSATGEINPLAEMP"
     gene            complement(25431..26555)
                     /locus_tag="VFC01_19840"
     CDS             complement(25431..26555)
                     /locus_tag="VFC01_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN81916.1"
                     /translation="MLCGMGDSTLTTELGRVLVTGGSGFVGANLVTELLDRGHHVRSF
                     DRAPSSLAGHPRLEVLTGDICDQQTVAAAVEGIDTIFHTAAIIDLMGGASVTEEYRQR
                     SFAVNVEGTKNLVHAAQAAGTKRFVYTASNSVVMGGKRISGGDETLPYTERFNDLYTE
                     TKVVAEQFVLSQNGIDGLLTCSIRPSGIWGRGDQTMFRKVFENVLAGHVKVLVGSKKV
                     KLDNSYVHNLIHGFILAAEHLVPGGTAPGQAYFINDGEPINMFEFSQPVVDACGQPWP
                     KLWVPGRLVKVVMMVWQWFHFRFGLPKPLLEPLGVERLYLDNYFSIAKAQRDLGYQPL
                     FTTEQAMADCLPYYVDLFHQMKGEAHEPAVSVVTASPLEG"
     gene            complement(26607..26819)
                     /locus_tag="VFC01_19845"
     CDS             complement(26607..26819)
                     /locus_tag="VFC01_19845"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZN81917.1"
                     /translation="MKPISQLGYEEARDELIDVVQQLEQGGLDLDASLKLWERGEELA
                     KRCEEHLAGARKRVEDALASKDDDDN"
     gene            complement(26816..28039)
                     /gene="xseA"
                     /locus_tag="VFC01_19850"
     CDS             complement(26816..28039)
                     /gene="xseA"
                     /locus_tag="VFC01_19850"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZN81918.1"
                     /translation="MTAPEPGQSPENPWPVRAVATRVAKWIDRLGAVWVEGQLTELKV
                     RADSKTVFMVLRDPAADMSLSLTCPRDLVHNAPVKLSEGTQVVVCAKPYFHTGRGSFW
                     LRVSEIRAVGVGELLARIERLRQLLYAEGLFDPRLKRPLPFLPGTIGLITGRASAAER
                     DVMTVATGRWPAVRFAVRNTVVQGPNAVPQILDALRELDGDAAVDVIVIARGGGSVED
                     LLPFSDETLCRAIAACTTPVVSAVGHEPDNPLCDLVADVRAATPTDAAKRIVPDAAAE
                     QALITDLRRRCARALRNWVHREQHTLSQLRSRPVLAQPLAALTARADEIHRARACVRR
                     DITRLVAAESDRIGHLSARLTTLGPAATLDRGYAVVQTASGAILRTTADAPSGTRLRV
                     RVADGAVTAVSKGKE"
     gene            complement(28036..28593)
                     /locus_tag="VFC01_19855"
     CDS             complement(28036..28593)
                     /locus_tag="VFC01_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid droplet-associated protein"
                     /protein_id="HZN81919.1"
                     /translation="MATAPYGVRLLVGAAVTAFEETRKLPQTILMYPMTVASQIAHLV
                     MKVQQDVADLVIRGDEALDQLFPPKDEQPEWATFDEDLDDNGLATPSSDGERLTEGRF
                     ALFTDGEPDGADAKPSNASTAADSPDVVAELDYESLTLAQLRARLTSLRVADLEALLA
                     YEDATKARAPFQTLLSNRITRATAK"
     gene            28660..29661
                     /locus_tag="VFC01_19860"
     CDS             28660..29661
                     /locus_tag="VFC01_19860"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZN81920.1"
                     /translation="MPPTINMGIPGASSSVTGGVTGKRVLLAEPRGYCAGVDRAVETV
                     ERALEKHGAPVYVRHEIVHNRHVVDTLAKAGAVFVDETDEVPEGAIVVFSAHGVAPTV
                     HVNAKERSLQVIDATCPLVTKVHNEAKRFARDDYDILLIGHEGHEEVIGTAGEAPDHV
                     QLVDGPDAVDNVTVRDEDKVIWLSQTTLSVDETMETVQRLRQRFPKLQDPPSDDICYA
                     TQNRQVAVKAMAPECQLVIVVGSRNSSNSVRLVEVALGAGSDAAHLVDYADDIDPAWL
                     SGVTTVGVTSGASVPEILVRGVLERLADYGYDVVQPVTTANETLVFALPREIRPARA"
     gene            29742..31466
                     /locus_tag="VFC01_19865"
     CDS             29742..31466
                     /locus_tag="VFC01_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003934044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZN81921.1"
                     /translation="MLAGCGDDATDRLNAVVHTFADSLNNGDAQAAAAVTTDPAQASD
                     TLGKLFDSLGRNAHFEVSTVDRKDNDATFGLSATWKFGKDGRNQWTYTTTGSAGQSGD
                     QWKIQWNPATVAPGLGTGPLSYSALTPDPAARVLDRAGAELLTQQVVTLVNLSPGADV
                     NAVAALLNPIDAGITAESLQTQLNAANGQQVTAVTLRAEDLAPIEEGLSALPNVTLAP
                     QTRLLSTDKALTSPTLSGLTDLWQRATDDAGGWAVSTQTASGSQRVGGQDPKPVSDIA
                     STLDIGMTRAARDALAPISTPAAMVVIQPSTGDVLAIGQNAAADAQGPIALTGLYPPG
                     STFKTVTVSAALQAGQVTPDSIVACPGTENIEGRQIPNDDNFDLGQVPLHTAFARSCN
                     TTMGRLAVNLPPDGLTKAAAQLGLGIDYTAPGMTTVTGKVPVADTSALRVEEGIGQGQ
                     VTASPFGMALVAAALAHGSVPSPTIVAGQPGKPDHTPDPLPASIDDQVKSMMRETITS
                     GTATQLQDIPGVLGKTGTAEYIDNTHAHGWFVGIDGDLALAVFVSDAGSSGPAADAAG
                     RFLRAQHP"
     gene            31546..>32509
                     /locus_tag="VFC01_19870"
     CDS             31546..>32509
                     /locus_tag="VFC01_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81922.1"
                     /translation="MGSPSSVDTPPSAASESWMRRLARTTPGVIGIIALAVAAICVIA
                     GVVCGGQLDGRIDERNAVLTSSEPFAYAAQNLYAALSAADAAAASAFLSGGIETAEMR
                     AKYQRALADAASALADTTAGTTDPATRTSLADISAGLSAYTALIESARANNRQGFVIG
                     SAYLREASTLMQTSLLPGAEKIFTANLATVDKDQRAVGSLPIASLVLLTLALVAIGVA
                     SMIMYGRTNRQFNIGLVIAAATVVLVIGWVVVATQLAAGEIEHSRAEGTARFEQLAKA
                     RINAQQARTVETLELIARGDITAREKSFHGHIDELGKLLDAAPAA"
BASE COUNT         5531 a        10586 c        10692 g         5700 t
ORIGIN      
        1 caaccacacg tgggaacacc ccaacatgac ggccatcccg ccggaggaca tcgctggcca
       61 gatttccaag gccaacgccg ctattcaagc ggcgaccggc cagcgcccta atctgttgcg
      121 caccgcgggc gggctgatca acgacaacgt gctcgcggcg gccaagcagc agggcatggc
      181 cgacatcaac tgggacgtca tccctttcga ctggatcaac gactccaaca ccgccgccac
      241 cacctacatg ctcaagacgc agatcaaacc cggttcggtg gtgctgttcc acgacaccta
      301 ctcgagcacc gtcgatgtgg tgtaccagtt catcccggtg ctcaagtcca acggctacca
      361 cctggtgacc gtcagccagc tgctgggtcc ccgcgaaccg ggcagctcct acggcagccg
      421 ggacaacggg ccgcctgcga acgacatcac cgacattccg ccagctgaga tcccgacgct
      481 gccgaacacg ccgtcgccca agccgatgcc gaacttcccg atcaccgaca tcgagggcgc
      541 caactccggc ggcgccaaca acggggctta gccgagctca cggcgcaagg tatgcgcacc
      601 gatacggcgt tcgtgctcac cgtcttggtg ctgtgctacg ccgtggtctc cgggctggtg
      661 aagcggtggt acatcgctcc cgcgttgatc ttcgtcgggt tcggaatggc gttgggcccg
      721 ttcggattcg gggtgatcga agccggggct gacacggcga gcttcacagt gctggcccag
      781 ttggcactga ccgtgatcct gttcaaccag gccgccatgc tcgatctgcc gtccgtcgtg
      841 cgccgcggcc acatcacctt ccggttactc gtgatcggaa tgccgctggc catcgtcctc
      901 ggcaccgtgg tggcgctctt ggtgctgccg cagatgccgc tgtgggaagc ggtatgcgtc
      961 gccgccatcg tggcgccgac ggaagtggcg ctcatcgacg cgctgctcga ggaccgccgc
     1021 attccggagc gcgtccggca cgcactgtcg gtcgaaagcg gatgctacga cggggtcgcc
     1081 ttggcggcga tgctggccgc cctcgcactg gcctctgagc agaacgaccc tgaccccgct
     1141 cgctggggat ggttcgccgt gcgcaccgaa gtggtgtcgc tcgccgtcgg gctcggtatc
     1201 ggcgtggtcg gtggcctggt gatcgtgcgg tcccggcagc gcggctggat gagcgacacc
     1261 tgggctcaac tcgccactgt cgcgttggcg ttggtgtgct tcgaattcgg cgagcgggtg
     1321 cacggtagcg gtttcgtcac tgccttcgcg ggcggtctcg cgtactcgat gatggtgcgt
     1381 cgggcgggcg cgcagatgcc gtcgcaggtc accgacgctg ccggtcagct gttggagttg
     1441 atggtgttcg cgatgttcgg cagctacgcc gtgatcgtgg ggtggcgcga tgcggactgg
     1501 cgggtggtgc ttttcgcggt cgttgcgctg atcctggtgc gactcgtcgc ggtgtcgaca
     1561 gcgctcctcg gcaccgactt gcccgctcgc agcaggctgt tcatcggctg gttcgggccg
     1621 cgcgggatcg gcaccctggt cctcgggttg ttgatgatcc agcgaggcga gatcgagcag
     1681 tccgcagtga tcacgcaggc ggtcgtcgtc accgtcacgg tcagcctcgt ggtgcacagc
     1741 ttcaccgcac cgctcggagt tcggctgtgg cccgccccgt cgccggagaa gacgacgaaa
     1801 gcgaacggct aggggcgccg tcctcctcga accaggaacc accccgcggc cgcggcgccg
     1861 ctcaatgcca ccacccggtc ggcccgatcg gccctcggca ggtccagcag cgagaccaag
     1921 cccagcagcg caaagccggt gcgcagagcc ggctggctgg tcccggtcgt caccagggtc
     1981 gctgggttac gggtggccgg gtcgagggtt acggtcaccg atcctcctgc agatgaaatg
     2041 tccaactaga gccgatgtcg acgctaattc gctcaagagc gttgataaat agggtgtttc
     2101 cctattcctg agccggttca ggtcttgccc atgtgtcggc cgagttcggc acgcgatttg
     2161 atgcccaact tgcgatagat ccgggccagg ttcgcctcga cggtcttcgg gctgatgaac
     2221 agcgtggtcg cgacgtcacg gttcttcatg cccgacgcgg ccagctcggc gacccgttgc
     2281 tcagacggcg tgagctggct gctctgccgc gggccgacat tagcccgggc gagttcggcc
     2341 ctggcgcggt cggcccacag ccgcacgtgc agccgctcga agatgtccaa cgcgtcctgc
     2401 aggctcgccg acgcggactc cttcctgcgc tgccgccgct gtaattggcc cagcagcaat
     2461 aacgttcgcg cacgttcgaa cggcatcggc agccgatcgt gttgagcgag cgcgcgcgtg
     2521 gccgctgcgc tggcggcgtc gaggtcaccc cgggcggcca gcagcatgct cctgccgcgg
     2581 gccccgaccg cgatcatcca cgcacggttc tgctgctcgc cgttgcgctc gagcgcgtcg
     2641 atgaggggct ccgcctcggc gccccgatcc acccgaatca gggcttcgat ggcgtccgtc
     2701 aggaaggcgg cactgggaag ctcggtgggc gtggatgccg gatcgaactt ggtcaccagc
     2761 ggctgcagcg tagcgaccgc ggcctcgtaa cgacccagtg acacctccag gaatccaagt
     2821 gccgcaacga cccggtccat caagcgaaat gccccggtgc gtctgccgaa cgccagtgcc
     2881 gcttcggcgg cttcgcgggc atcggcttcg cggcccgcgt atatggccaa cttcgcccgc
     2941 aggctgtgcg ctatgaacag cggcgtgtcg ccgccgagct ggcgggctcg ctccatcgca
     3001 tcttcggcga ccaatccggc ctcgacgaaa tcgccccgcc aaatgctgct cagcaccaca
     3061 tgctgggcga tgaagatgag ttcgccttcc tcgcccgtat ccgcgcagcg gcgccggacg
     3121 ccttccagca ctccatgggc ctcgtcaggg cggccggtcc actccaacag caaggcacgc
     3181 tgaacggacg gccgaaatac caagggggtg aagatgttcg ggtcttcaag ctcaatagcg
     3241 cgacgcaggt ccgcctccgc gaaacccttg cccgacagga accgcaggat gacacgcata
     3301 ccgagcgcca ggcccagcag gtggggctgg ccgaggcgct ccgccttggc gacggcctgg
     3361 tcggccatcg tcacgctcgc ttgggcctga ttggcctgaa gcagggcata cgccaatgtg
     3421 atcaatgtct gcacgcgcag cgggctgtcg gcgtgcacct cactgagggc ctgctgcagc
     3481 aaccgggcgc cttcgtagaa cccgtccccg tacaggcgca cgatagcgag ccggctcaac
     3541 gcttcggctc gcaggtcacc cggcgccagt cgctcgatgg tctcttcgag aacggcgcgg
     3601 gctcgctcct ggtcgcccgc atcgaaatgg tgtagagcag aacggagtcg acgctggggg
     3661 gtatcaccgc cgaggccgat cgccagatcc atcagctcag cggccgccgc gggtgccccc
     3721 cggacgcgcg cggactccgc cgccgtgtcg agcgcccgca gtgtgacttc gtccccactg
     3781 gtcgcagcca aggccagatg ccgagcgcgc aactcgggct cgtcgacgat gtcggcgagg
     3841 cgacgatgca tcgtccggcg atggcccggc gtggattcgg tgtacacacc ccgggccagt
     3901 aggggatgag cgaagtagat ccggttgccg tcgatcgcga ttatgccctt gctttccgcg
     3961 ttctcgagca gctcgaccaa tcggtcgtcg tcgctgactg tggcacttga caccagctcc
     4021 accgttggcg acgccatgca cgacgcggcg agcagcgcat catggacgtc cggatcgagg
     4081 ccgttgatcc tggtccgcac gacgtcggcc agtgtacggg gcagtgacgt ctcgacgccc
     4141 ggagctcgtt cggcgatcgc gcgcgccaat tcgattgcat agaaggggtt tccgccggac
     4201 acctcgtgta tccggcccat cgtcggacgg gagaacggtc gccgcagtcg tgtcgaaact
     4261 gccgcatgta gatcatgaat gcctaatggg ttcaaccgga tccggctcac ggcatcgggc
     4321 cgcggcagtt gcacccaagc gccgccgata ccgtcgccat cggttcgcac actgccgaga
     4381 atgccaacgt gcccggtgag tcgccgtgcg gcgaacgcga ccacatgcat gctcgacggg
     4441 tcgatccatt gcaggtcgtc gatcgccacc agaagcggcc cctcgtcggt gaggtactcg
     4501 accaccgaca ggaacgccgc agccacggcg cgctgatctg tggccgatcc gttgtcggcc
     4561 cgcatcagca cctgatccac cgcgaagcgc tgggggtcgg gcaagtccgc ccacgcggat
     4621 gcgggcacac catcgagcag atcggccagg gccgtatagg ccagcacgga ttccgccgcc
     4681 gccgcgcgcg ccgacaagac accgaacccc tggtttcgtg ccagctccag cgcctctaac
     4741 cagagagtgg tcttgccaat gcccggctca ccctcaatca gcaaggccga tggctcggta
     4801 gccgcagaag ccaaaaaccc agcgatcctg cggctctctg tctgacggct ctgcaccccc
     4861 actcccgaca catctacaaa caattccagc gaacggcggc caggtctact ttttggccca
     4921 actaggcagt accaccgccg ctggcaacat ccggaacagc ttgcttaccc gctagcggcg
     4981 gatcgtattc atgtaccgcc cgagctgggc ccgcgagtgg ataccgagct tgcggtagac
     5041 acgtgcgagg gtggcctcga cggtcttcga gctgatgaag agcttggccg ctacatcccg
     5101 gttcgtcata cccgacgccg cgagttcggc cactcgctgt tcggcggtcg tgagcgagac
     5161 gtccgtgtgg tcccgtgagc gggtgcccgc gagttgggca cgggcgcggt cggcccataa
     5221 ctgagtaccg agctcctcga aggtggccag ggcatcacgc aggacggcgg ttgcctccga
     5281 ccggtcgcga cgaatgagtt gcccaagcag taactgagtc cgtgcccgct cgaacggcat
     5341 cggcagccgg tcgtgctcgg tcagcgcccg ccgcgcgctc tgcaccgccg cttccacgtc
     5401 gccgcgggcg gcctgcacca tcgcgcgacc gcgtgctccg accgcaagca tccacgctcg
     5461 gtcgagtctg cggccgttgc gctcgagcgc gtcgacgagc ggtacggcct cattcgcgcg
     5521 gcccactgcc gtcaacgctt ccaccgcgtc ggggataaac gacgccatga cgatctcggt
     5581 gaggttcggc gacagtccgg acctcgacag cagtggttcg agcgcgctca gcgccgcgtc
     5641 ataattgcca agcgacgtct ccaggaagcc cagcgccgtg agcgaccact cgtcgtgcca
     5701 tccggtaccg ctgcgcttac tggcgtcgat tgcgtccgcc acggcgggcc gggcgtcgtg
     5761 ctcatgaccg gaaaagacgg cgagccatgc gcgcaggacg agggtcaaca tggcggggaa
     5821 gtctccgccg agctggctag ccagttccgt cgcggcctcc gtcacccgcg aggcttcggc
     5881 gaagtcacca cgccaaatcc ggttcagcac aacctgaaag tcaatgaaga tcaggtcgcc
     5941 ttcttccccc cgctcggtgc agcggcgctg gattgcttgc atccgttgat gggatgtgtc
     6001 caggtcgccg gtccagccca ccagcagcgc gtgttccaca ctgggcttga gcaccgtcgg
     6061 agtaaacgac tcgtggtcct ccagctcgag ggcgcgttgc agcctcggtt cgtcaacgcc
     6121 gtcgccacgc aggaacaaca gcatcgaccg cgcgccgagc gcctggctca acaggcctgg
     6181 aacgcccagc tgctcggcca atgtcaccgc ctgctcggcg cactgccacg cagtgtccgg
     6241 ctcaccggtg ttgtacaggg catgcgcaag tgccgtcagc atgcgaatgc gcaaggcctg
     6301 accgggccgg tcctcggcga gtgcgcgctg cagcacctgc gacgcttcgg ggtacccgtc
     6361 atcgttgaat atcacgaccg ccaacgaatg caacgcttcc gcgcggaccg gtcctggttg
     6421 cagttccgcg atcgcacgct gcaacagtgt ccgcgcccgc gcaagatcac cgccgtcaaa
     6481 aacatgtccc gccaaccgga ctcgacgcag cgggatgtcg ccgcccaggg cgatcgccag
     6541 ctctaaaagc tccgctgctg cggccggtgc gccgcgcgcg tgcgctgaag ccgccgcgtc
     6601 atcgagcgca tcgagagtct cggggtcacc ggtggtcgac gccagtgcga ggtgccgtgc
     6661 gcgcagctcg acttcgtcga cgacgcctgc gaggcgacgg tgcatcaggc ggcgttgcgt
     6721 cggtgacgcg ccggtgtaca cgctgccggc caacaacgga tgcgtgaacc ggatccggtt
     6781 gccttggatg gagatgacgc cgtggcgttc ggcagcatcg agcagctcga tcatccggtc
     6841 atggtcggtg ttcgccgctt cggcgaccag ctcgacggtg ggagtggcta cgcaggcggc
     6901 agcaagaagt aagtcttgga cagctggatc caagctgccg accctggcgc gaaccaactc
     6961 agtcagcgtg ccgggcagta catcaccagc attatcggtt agcgccgcgc gggccaattc
     7021 gatggcatag aacgggtttc cggccgagat ctggtgaatt cgcgccatgg ccgcgcgggg
     7081 tagggtacga cccactgcct ccttgacaac ggtgtgtagc gcgggttggg tcaagggacg
     7141 caccgtgatt cgttggattg catcgggccg gggcagttgc agccacgaga tggcgtcgct
     7201 gttgttgggc tcggtgcgaa cggtggccag gaagcccacc ggacccgaca gtcgccgcgc
     7261 agcgaacccg atcacgtgcg cgctggacgg gtcgagccat tgcagatcgt cgatcgtcag
     7321 cagcgcaggg ccatcgttgg tgagccgctc gacgaccgaa aggaatgcag ctgccaccgc
     7381 gcgctggtcg gtgacgacgt cggtgctctg gtcgcgcgag tgcactcgat ctatcgccat
     7441 ccgctggggg tcaggcaggt cggcccaggt gtcctggtcg atgccggtca gcaggtcggc
     7501 cagggcggta tacgccagga cggattcggc gcccgcagcg cgggcggaaa gcacctggaa
     7561 cccgcgatcg cgtgcgcgtt ccagcccggc cagccacagg gtggtcttgc cgatcccggc
     7621 ctcgccctcg accagcagcc cggacggcgc gctgcacgcg gagtccagga actcctctaa
     7681 cgccccgtcc tcggaggggc ggctagccag tccagcggtc acccctgaag aatctcagct
     7741 accgcggaca ctggttccaa tttcaacgta tttgtcgggc gaatgccgca ggttccgacg
     7801 cttggtggtg gatattgcct gttcatggcg gcgtggatgg tcctcacccg ggctggcggc
     7861 aatgtcggtc ccgcgctgtt gcgtcggctc accctggtca gcagcgaatg cccgggaagc
     7921 ctacgcgtcg ccgtccttga ccttggccat cgccagcacg tcgaggcgct tgtcgagttc
     7981 ctccagcgac agcttgtcac cgatcagccc gcggtcgatc acggtctggc ggatggtctt
     8041 cttttccttc agcgcctgct tggcgaccgc ggccgcttcc tcgtaaccga tcgcggagtt
     8101 cagcggggtt acgatcgacg gcgacgactc ggccagctcg cgcagccgct cttcgttggc
     8161 gaccagaccg tcgatgcaac gctctgcgaa cagctttgag gcgttcgtca gaatcttgaa
     8221 cgactccagc aggttgcgcg ccatcatcgg gatgtacacg ttgagctcga aagcgccgtt
     8281 gccaccaccc cacgcgatcg cggcgtcgtt accgacaacc tgcgcggcca cctgcgtaac
     8341 cgcctcgggg ataacgggat tgaccttgcc cggcatgatc gagctgcccg gttgcagatc
     8401 cggcagctga atctcgccga gaccggtcag cggacccgag cccatccacc ggatgtcgtt
     8461 ggcgatcttg gtcagcgaaa cggcgatggt gcgcagcgca ccggatgcct cgaccagccc
     8521 gtcgcgcgcc gcttgagcct cgaaatggtt tgccgccacc cgcaattcgg cgatgccggt
     8581 ctgctcggtc aacaccgcca ccacgcgctc gtcgaaatca tcgggcgcgt tcaggccggt
     8641 gccgacggcc gtgccgccga tcgcgagctc acctagccgc ggcagtgtgg ccttcacgcg
     8701 ctcgatgccg gcttcgacct gacgggcata tccggagaac tcctggccca gcgtcaccgg
     8761 cacggcgtcc atcaggtggg tgcgccccga cttcaccacc gtgcgccatt gccgcgcctt
     8821 gccggccagc gactcgtgca acacctccag cgctggaatc aggtgccgca cagcggcttc
     8881 cgtagcggcg atgtgcgtcg cggtggggaa ggtgtcgttg gacgactgcg acatgttgac
     8941 gtcgtcgttg ggatgcaccg taacgccgtt ctgcgcggcg atgccggcga tcacctcgtt
     9001 ggtgttcatg ttcgagctgg tgcccgatcc ggtctggaac acgtcgatcg ggaactgatc
     9061 gtcgtgaaga ccgtcggcga tctcaccggc cgcggcgatg atcgcgtcgg ctttctccgg
     9121 cgcgagcaac ccgagatcct tgttcacttg ggcgcacgcg cctttgagca gcccgagcgc
     9181 ccggatctgt gtgcgttcca gaccgcgccc ggagattggg aagttctcca ccgcccgctg
     9241 ggtctgtgcg cgccacagcg cggtgatggg cacccggacc tcgcccatgg tgtcgtgctc
     9301 gatgcggtat tcgacgtttt gggaggtgct tgaggcgacg ctcacgaaaa atcctcgcta
     9361 atggttgggt cagggcagcg gctggtaggc gttgctgtcg ccggtgaaat cgatggcgga
     9421 gtattcgttg agcttggaca accggtggta tgcctcgatc atgcgcacgg tgcccgactt
     9481 gctgcgcatg acgatcgact gtgtggtgca tccgccaccg tagaaatgga caccgttgag
     9541 caggtcaccg tcagtcacac cggtagcgga gaagaacacg ttgtcgccgg acaccaactc
     9601 ctcggtcgtg aggatctggt cgaggtcgta gcccctgtcg attgccttct ggcgctcttc
     9661 gtcgtctttc ggccacagct tggcctggat cgcgccgccc atgcagcgga tggcggcagc
     9721 ggcgatgatg ccctccgggg tgccaccgat cccggccagc atgtcggtgc tggtgttggg
     9781 ccggcatgcc gagatcgcgc ctgccacgtc accgtcggag atcagtcggc accgcgcgcc
     9841 ggtgtcgcgc acgtcggcga tcagctgttc gtgtcgcggc cgatccagga tgcatacggt
     9901 caggtcggag actgagacgt ttttggcctt ggctactcgg cggatgttct caccgatcgg
     9961 cgcggtgatg tcgatgacgt cggcggcgtc gggcccgacg gcgatcttgt ccatgtaata
    10021 aaccgccgac gggtcgtaca tggtgccgcg ctcggacacc gccagcaccg agattgcgtt
    10081 ggccaggccc ttggccatca acgtggtgcc gtcgatcggg tcgacggcga agtcgcactc
    10141 gggcccgtct ccgttgccga cgtcctcgcc gttgtagagc atcggggcgt tgtccttctc
    10201 gccctcgccg atcaccacga cgccgcgcat cgaaaccgag ttgaccagct cacgcatcgc
    10261 gtcgaccgcc gcgccgtcgc caccctcttt gtcgccgcga cctacccagc ggcccgccgc
    10321 catcgcgccg gcctcagtca ccctgacaag ttcgagggcg aggtttctgt ctggggcttc
    10381 accacgcgct gggctcatgg cgcgattctc ccattagcag gtcggcgttt ggcagctggg
    10441 atactggcgg ctatgacgac ggagccccta ccggccccca agcctcaaaa gccgcggata
    10501 ctgcaggacg gccgggacat gttctggtcg ctcgcgccgc tggtgatcgc ctgcattgtg
    10561 ctggcgggga tgctcggcat gtgctcgttt cagccgaacg gacctggcaa gggaccggcc
    10621 ccgtcctacg acgcgcccgc cgcgctgcag gccgacgccg acgctctgaa gatcccgatc
    10681 cggctgccca ggctgcccga cggttggcag gccaactccg gtagccgcaa gggcattgac
    10741 ggcggccgca ccgatcccgc gtcccggcag ccggcgcgcg ccgtcagctc caccgtcgga
    10801 tacctcgcgc ccactgggat gtatgtgagc ctgacgcaga gcaacgccga tgaggacaag
    10861 ctcgtcgagt ccatcaacac caacctctat ccaactggga cgcaggaggt cgacggcgtg
    10921 aaatgggtgg tgtacgaggg tggcggccag gatggcaagc cggccgagcc gctgtggacc
    10981 actcggctga acgggcctac cggaccggcg cagattgcca tatcgggtgc cgcgggtacg
    11041 gacgagtacc gtacgctggc tcaagcgacg cagacccagt cgccattgcc cgccaagtag
    11101 gagaatccat gaccgcgccg gcagcagatc tgaccgggtg gaccgtcgcg ccgttttcgg
    11161 cggccggcta cacccacgac gtgtaccgca agggcgaggg gcctggtgtg gtactgatcc
    11221 cggagttgcc agggatgagt cccgaggtta tcgggctggg taatcacttg gtggacaacg
    11281 ggttcaccgt ggcgtgcccg tcgctgttcg gcactccggg tgccgcggct gtcaggccgg
    11341 gcgcgatacc agtcatggtg cgtgcctgta tctcaaggga attcgcgggc ttcgcactca
    11401 acgccgaccg tccggtggcg cactacctgc gtgcgctcgc ccgtgacctc aacgagaaga
    11461 cgccgggcaa gggcgtcggc gtcatcgggc agtgcttcac gggcgggttc gcgcttgcgg
    11521 ccgctgtgga cgacagcgtg ctggcacctg tgctgagcca gccgtcgacg ccgatttcgt
    11581 tgacgcccaa gatgaagcgc gaccctgggc tgtccgaagg tgaactcaag gtcatcgaac
    11641 agcgcgccgc caacgaggga ttgtgtgcgt taggcctccg gttcagcgag gacccactgg
    11701 cgccggggga gcggttcaag acgctgaagg ctcgacttgg tgacgcgttc gaggtcatcg
    11761 agatcgactc gaagaagggc aacgaacaca gtttcggcaa gaggacgcac tcggtgctga
    11821 cgctcgaggt ccgcgagcgc gacggacacc cggcctacga ggcacgcaag cgggtcgtgc
    11881 agttcctcaa ggagaggttg acttagccgg agcctttgcc ggcttgttct tcccgctgcg
    11941 acgggcccac cagccgagaa gcctgcgctg ctttggcttt tcgtcatcgt cgggttcttc
    12001 catcggcacg tccttgtaca ccgcgaggta gacgctgatc gtagtgacga tgacgatcat
    12061 cagcaccggg ccgatcacaa tgccccatgc gccgaacatg gcaatgcccg cgaatacggc
    12121 gagcagcatc agcgccgagt cgaggcgagc ggcccgcggt accaggatcg gccgcaggaa
    12181 gttgtcgatg ttcgtcacca cgatcaggtg gaacaggatg acgaacacac cgccgaatac
    12241 gttgccgaac aggatcattc cgatcccgaa cgggatggtc acgatgccgc tgccgagcgg
    12301 gatcaccgac agcgcggaca gcagcaccgc gaaaaggaag aaaccctggt ggaacccggc
    12361 gatgtagatc gatgtagcgc ccgcgacacc ctgacagacg gcgatgacga attggcccat
    12421 tacggtgccc ttcaccatcg cgcccatctt cgacatgtag aggtcggtga tttcctcgcc
    12481 gagtgggttg agccggcgga tgagaaggag caccttgtcg cggttcgtca gcagcgagag
    12541 gaacacgtag agaaacagga tcgcggcggt caccccgccg aacacgctgc cggccgctcc
    12601 ctgcagcagg cccaacagcc actcgccgac cttttgcgca gccgtggcca tcgaaccccg
    12661 cagcgaatcc ggcgtgaccg tcacgttgat gaacggcacc cggtgcagca catcgttgac
    12721 gaagcgcagc gcctgatcgc cgagcgccga caggtcggtg cgaccgaccc agtccgccac
    12781 tcgctcgacc atgttggtga tctgcacgac ggcgagcatc acgaacagac ccatcggcac
    12841 aaccacggca gcgatcgccg ccaagagcgt cagcgtcgcc gataacccgg tgccgaaccg
    12901 cttgttgagg cggttgaaca gcggcgtgaa caggtaagca gccaccgccg ccaccacgat
    12961 gagaatgaag tagccacgca ggaagtaagc gccgaacagg atggcgatca ccgtcgcgac
    13021 ggccagcgca cgcttctgtg tcagcgtgaa ctcggtgtcc attgagcgct ccctggggac
    13081 tacagtgttg agccgtcgtg gcgactgggc gtgatgaaca gtatgtggtc ctgagtttgt
    13141 cggtgccgat tcgtaatgtt tgtggcatgt ccgagcgctc gtttgcccgc acatctggga
    13201 gtgcggtcgc tgtgctcgcg catcgcgggg tgctcgaagt atcagcgacg ggttgtagct
    13261 gggcaagctg cgtgccgaca gctgtcggaa tggatcaacg acgaccgcgg tgcacagatt
    13321 gtggcgtggg aattcgtatt ggatcacctg cgccagatcg tgtgggtcgg ttcaaccgat
    13381 gcggccgcga cggtggtttg ggcattcacg ggccgcaccg caccggtgct gtgacaagag
    13441 gtatcgcacg tgcgtgggtc ggtcgaggtg ttctgctccc tatcgcatgt cgccaccgat
    13501 ttaggtgcgt ctcagagctg aggacactta gttacatcag gcgccggttg aacgtggggg
    13561 caccgtgaga cgcgcgtacg tgttccgatt gcgccccacc gcgcgccaac atgttgtatt
    13621 ggcggcttgc gcggaatcgc atcgggagtt gtacaacgcg gcattgcagg aacgccgcga
    13681 tgcatgggcg cacaagaccc gcataagtta tggcgatcag tccgcgcagc tgacagagat
    13741 gcgctcagtg cgcccggatc aggcggtgtg gtcgttttcg agtcaacagg ccacgcttcg
    13801 tcggctagac aaggcgtttg cggggttctt ccgccgcgtc aagcgcggtc agaaggcggg
    13861 ttatccccga tttaaggcca cggcgcggtt tgacagtgtg gagtggccca aggacggtga
    13921 cggtgcccgg tggctgcccg agcgacggcg ggtgtatcta aagggcgttg gggaagtaaa
    13981 ggtcaacctg catcggcaag tcgaaggcca ggtgaagacg atccagatca agcgtcaggg
    14041 tcggcactgg ctgctggtgc tctcgtgtga tgacgtgcca acccgtccgc tgccggctac
    14101 cggccgtcag gctggcgtcg atgtcgggat cgtcagtttc gctaccatga gcgacggtga
    14161 gcgcgtggca aatccgcgct ggggccgtgt cgcggggcgt gggctggcca gcgcgcagca
    14221 gcgactagcc cgcgcaaacc gcggttcgaa caaccgcgag cgcaagaggg aaacggtggc
    14281 agccaggcat cgcaagatct ccaaccagcg caaagacttt caccacaagc aggctcgcgc
    14341 tctcgttgaa cgctatgacc tgctagtggt ggaggatttg cagatcgtca acatggtacg
    14401 ccgggccaaa cccgtgcccg acccagataa ccccggccag tatttggcta atggtgcccg
    14461 ggcgaaaacc gggctgtccc gaagtttcag tgacgccggc tggggccggt tcgtctcgat
    14521 tctgcgcgcc aaagcggaag aggctgggcg catctggatt gaggtcgacc ccaggcacac
    14581 gtctgaccgc tgcgaacatt gtggacacgc agactcgcga aatcgcgtca cgcaagcggt
    14641 cttcgaatgc cagcgatgtt cgcatcgcgc tcacgctgac gaacacgccg cgcgtaatct
    14701 cttacgggct ggactggccc ttcacgcaca agccgcgtga aaagaagccg gcggcctcca
    14761 gtcgccggag aagtcactgc tgtgacgcta gctcacctca gctcagctgg gaacgtcctg
    14821 tggcctggct ggcgcgctgc ttttcgatga agccgatcga catgcgctgc atcagcgtgg
    14881 gcgaaagccg ggcgaacagc cattgggcat gtgccagccg cggtttcacc aggatcgcct
    14941 tgttcttctc gatggcgcgc aacgtatccc gcgccagccg gtcggggtcg tacgcggtct
    15001 tcacgccctg gccctcgaga aagtagttgc ggccgacgaa tccgccgatc gcccccttgt
    15061 cgaggatcgg ggtctcgacg gcggagggac acaccaccaa gaccccgacc ccgcgggaga
    15121 tggcctccga acgcaacgcc agggacagcc cgacaacggc gtgcttggtc gtgacgtaac
    15181 tggtgatctg tccggccgcg gtcagccccg ccatcgacgc ggtgttgatg atgtgcccgt
    15241 ggccttgccg caccatcagt ggatacgccg cggcgacgcc gtgcacgacg ccgcggatgt
    15301 tgacgtcgat gatcgcgttc cattgctcca acgtgagcag ctcggtgtcg ccgcccaaga
    15361 cgatgcccgc gttgttgaac atcaggtcga gcgggcccgc gcggtcgacg acttcgtcga
    15421 cggcttgttg taccgccgcg gcgtcggtga cgtcgagctg tgcggaacgg gcggtggtgc
    15481 ccagtgccgc ggcggtgcgt tcggcggcgt cgccatcgat gtcggtgcag accaccgtgg
    15541 cgccggcgct gaccagcgcc cggcacagcg ccgcaccgat gccagagccc gcgccggtga
    15601 cgatcgcctg cttgtccgcg aaactcattg tgcctccgcc agtttcatca catggccgag
    15661 gatgtcgggg tagcgcttca agtgcgcgcc gccgttgagg tcgagcacct cgccggtcag
    15721 ccacgacgcc ccaggagagc agaggtacag cacggcgttg gcgacgtcct cgggcctgcc
    15781 cgcgcggccg agcgccgtgt tctcgaggta ttcctcgacc acgcccggaa cagcggccgc
    15841 cggctcggtg agtggtgtgt ggacgaagcc gggtgcgacg gcgttgacgc ggattccccg
    15901 cgggccgagc tccagcgcgg cgacctgagt gagcatcgaa aggccggcct tggccgcgca
    15961 gtacgcgctc atgcccgccg cgggctggcg cgcattcagc gagctgatgg agaccagcac
    16021 cccgccggtg cgcaggctgc ggcccgcgtg cttcgcgacg atgaacgccc cggtgaggca
    16081 gacgtcgacc acgtcgcgga actgatcgac aggcatgtcg gtgataaggc cgatgttgga
    16141 gaaccccgca gtgttgacga cgacatcgac cgggccggtc tgctcgaaga gccgctgcac
    16201 cgaattctcg tcggtgacgt tgacggccgc tgcggtgtgc ggcccgccga gttcgccggc
    16261 ccgagcttgc gcgccgtcgg cgtttcggtc ggcgacggtg acctggtcgc cctgggcggc
    16321 cagcgcctgg gccgtcgccc agccgatccc ggacgctccg ccgaccacaa cggcgcttcg
    16381 ctggttaccg ttgctcatac agttggccct tcgacgatga agtagaacgc gttccaactt
    16441 agccctttga cacactcgac ccgtgacaga tctgaagttg tccgattcga tttccgtggc
    16501 ggtgacaccg gacgagctgt atgcactggt gtcagacgtg accaggacgg gcgagtggag
    16561 cccggtctgc cgggcctgct ggtgggacga cggcgacggc ccgcgtgtcg gcgcgtggtt
    16621 caccggccgc aacgagcttc cggagcggac gtgggagacg cgctcccagg tggtggccgc
    16681 ggagcccggt cgcgagttcg cttgggaggt caacaacggc tgggtgcgct ggggctacac
    16741 cctcgaaccc gaagagggcg gcacccggct caccgagtcg tgggagttcc tgcccgccgg
    16801 tgtcgccggt ttccgcgagc gatacggcga gcacgccaat gctcatatcg agcatcggac
    16861 caatatggcc agaaccggca tcccggcgac gctcgcggcg atcaagaagg ccgccgaagc
    16921 ttgaccccgc ggtgcgcgaa cgttccatac cgctcgaaaa agtgccgaaa tttcgagccg
    16981 taaggaacgt tcggccacaa gtcagcggcc ttcgattgtg ttcccggcca catcaacggc
    17041 gaccgatgtt tgtcgccggc gccatcgact gacaggggaa cccatcgaac gatggagcca
    17101 cgacgatgtg gacctgacta ggcgagaggc gaggtgacat catcatggcc gacgacgcac
    17161 agcgacgaat cgcgcgattc atagaacctt ttcgagtcga tccgggctcg aaggtgaacc
    17221 tggcgaagga cttcgatccc gcgttcgaag cgggcatcaa gacgaaagac gacggcgtcg
    17281 agctgctgaa ggacggcgtc gagctccttg cgcaatacca ggcgcggctc gccgctcagg
    17341 acacccaagg cgtcctagtt gtcctgcaag cccttgatgc cgccggcaag gacggcacga
    17401 tccgccacgt gatgactggc gtcaacccgc agggcgtgcg ggtcgagagc ttcaagcaac
    17461 cgtcctctcg cgagctcgac caagacttct tgcgccgcta ccaggaacgg ttgccgtcgc
    17521 gtggggagat cggcatcttc aatcggtcgc actacgagga ggtgctcgtc gtacgggtgc
    17581 acccggaaat cctccgcctt cagaagctcc cggccgagac gctcgagcat cacgtgtgga
    17641 aacgcaggta cgaagcgatc aacgactggg agcggtatct gtcggagaac gggttccgga
    17701 tcgtcaaact ctttctgaac ctctcgaagg aggagcagcg ccagcggttc cttcggcgca
    17761 tcgatctccc cgaccacaat tggaagttct ccttgcacga tgtcgaggag cgccagtatt
    17821 gggatgcata ccagaatgcg ttctccgaga tgttcagtca caccagcacc gagtgggcgc
    17881 cgtggtacgt aatcccggcc gaccgcaagt ggtttgcgcg cactgctgcc gccgcggtga
    17941 ttgctaatgc gctgatcgac atcgacccgc agtacccgac cctcagcgag gacgcgctcc
    18001 atgacctcag tgcacgaaag gtggcactgg aatcagaggc gccctccgtg gcagcgccga
    18061 ctgcgaacgg cgccgcggcc cgcacaccga tcaagaaggg tgcctgagcg atgaccgcca
    18121 agttcgtggc agcgatcgac cacggtacga ccagcacgcg ctgcatgctc ttcgacgcaa
    18181 agggtcagcc ggtcgcgact ggccaacgcg cccaaaccat gtactacccg caccccggtt
    18241 gggtcgagct cgacatggac gaggtctggc gaggcaccca ggagtgcatc cacgacgcgc
    18301 tcgcctcggt gggcgcctct tcggcggacg tggctgggat cggaataacg aacgaacgcg
    18361 agaccgtcgt cctttgggat cgacggacag gacatccggt tgcgcggtcg atcacttggc
    18421 aggacacacg cacagccgac gcagccgacg cgctggcggc cgacggaggg atccaccggt
    18481 tccaacacca aaccggcctg ccgatctcga cgtattcctc tgcgctgaaa cttgcgtggc
    18541 tgctcgacga agacccggcc aggcgggagg cggccagacg tggggaccta ctcttcggca
    18601 cgccggactc ctggctcctc tggaacctga ccggaggtcc tgacggagga ctgcacgcga
    18661 ccgatccgac gaacgcaagc cggacactct tgatgaacct gaaaaccctc gaatgggatg
    18721 aagacctcct cgacttgatg gagatcccgc ggcaggtgct tccggagatc cgctcgtcca
    18781 gcgagatcta cggaatggcg gtcggtgatc tggccggcgt gcctgtcgcg ggcctcattg
    18841 gcgaccagca ggcatcactg ttcggccaca cgtgtttcga ccacggcgac atgaagaaca
    18901 cctatggcac cggcgcgttc atgctcttca cgctcgggaa cgaaccggtg atgtccgagc
    18961 agggcctggt tacgacggtc gcgtggaagc tcggcaacgc ggacccggtc tacgcgctgg
    19021 aaggctcggt cgcggtcgcg ggcgcactcg tccaatggct gcgcgacaac ctcggaatca
    19081 tccacgacgc cagtgagatc gagtccctcg cacgctccgt cgacggctca gaaggcgtgg
    19141 tcctcgttcc ggcgttctcc ggcttgttcg cgccgtactg gcgcgacgac gcgcgaggtg
    19201 tgctcgtcgg cctgacgcgc ttctccacca agggacacat tgcgcgggca gcgctcgagg
    19261 ccaccgccta ccagacatac gatctggcgc aggccatgct caccgacacc ggactcgcgc
    19321 aactggggga gttgcacgtg gacggcggca tgacgcaaaa cgatctgctg atgcagtttc
    19381 aagcggacat tctcggctct accgtcgcgg cccccgcctc cacccagatg accgccaccg
    19441 gcgccgcata tgccgcgggg ctggcaacca agttctggtc agatctcgat gagcttcgcg
    19501 cgaattacgc gatcacacgc cgctggcggc ctcagatggg cgacgacgag cgcgctcgcg
    19561 gcgtcgccgc ctggcacgac ggtgtcgagc ggaccctcca cctggcggag aggcagccgg
    19621 tcggaccggc ccggccggct agcgcttgac gcaccgaaag ccgatgtggc tcatgccggt
    19681 atcgacgggc tgcggtcggc gtgccgcggg tcgatagcgc aggcagtagt tgtcggcgca
    19741 taggaacgaa ccgcccttga cgaccttgcg cggcacgcgg aactgcggct ggcgggggtc
    19801 gtaactgtct gcctcgcaac acggatcacc ggcgcgggtg tcggcgtacc agtcggtggt
    19861 ccactcccac acgttgcccg ccatgtcgac cagtccgtag ccgttgggcg ggaagctgcc
    19921 gacgtcagta gtgcgcccgt agccgcggtc gggtcgccac ggaaaatcgc cgtgccaata
    19981 attggccagc ggctcgccgg gctgctcggg ttcgtcaccc cacgtgtacg ccgcaccgtt
    20041 aagcccgcca cgggccgccg tttcccattc cgcctcggtc ggtagcgaga gccccgccca
    20101 cgccgcgtac gcctcggcgt cttcgtaggc gacatgcacg acgggatgtt cggcacgctt
    20161 gtcgatcgac gagtgcgggc cgacaggatg ccgccacgac gcccccggcg tccacgtcca
    20221 ccacaggttc aggtgccgca ggtcgaccgg gccactagtg cgggtgaaca ccatcgagcc
    20281 gggctgcaga ttctcggcgg gcgcgtcggg atagtcagca gggttcagcg gcctctccgc
    20341 cacagtgaga tacccagtgg cagtgacaaa ttcggcgaac tcggtgttgg tcacctgatg
    20401 cgtctggatc cagaacccgt cgacgctgac gggccgcgca ggcccctcct cggcatagtg
    20461 gtgctcggat cccagcaccg cggtctgcgg tggaacccgg acgagttcgt tagtcactga
    20521 agacggtcgt ccagtcgttg cgcatgctga cgacggtcca gtcgtccttc gcggcttgct
    20581 cgagtgattt ctccgcccct gcgacgtaat cgaattcgcg atccttgtcg tcgtgcagca
    20641 ccagtagccg catgccgcgg gtgaattcca gcatttcgat gtcgccgttg gagttaccca
    20701 ccgccagaat cggcctgcgc ccgatccggc tccacagtcg cacgggtttc ataggcccgt
    20761 cgtcgaggaa ctcgggttgg tccgtcgtgt acaggtgtcc gtcgcggtag acgagaccta
    20821 ccgaactgcc caccactcgc tcggggggta ccccgtacat cggtccggtg accggccgca
    20881 tgaaatcccg cccgccgccg gacacgatgt aacagatgaa gccattggtc tccagatagc
    20941 gcagcaactc caccatcggc gcgtacccac acgacgtata ggcccggccg agcgtcggat
    21001 gcttggcttc ggcgaagaac gtgttgatcc ggccggcgtg ctcttcgacg gtgatctcgt
    21061 ggtgagccgt aagtatcgct cccataagtg gtttcagatc tgtgtcatcg ccctggtagt
    21121 gcttgctgac cgcgtcgccc aaccactgca ggtcgcctgt ataggcggct ttgtacggct
    21181 gctggtcgcg cagcgacgga tccgcctcgg cctgttcctt gaatcgtcgg agcagaaagt
    21241 cgagctggat gtacatcggc ttctcgcacc acagcgtgcc gtcgttgtcg aaaaccgcga
    21301 cacgtgcttg cggctcaacg taatccggtc cgccttcact ggtgacgcgt cccacgaagt
    21361 cgaggatcgc cgacttcgta ggtccgtcaa cccatgatgc gagttccgcc acgctcaacc
    21421 gccccgcgag gcgaggaact tgttgagctc ctccaccgcg tggttgatgg tgaacgacgc
    21481 cggctcctgc cgtggcggga actctttgaa ggtctgcagg aactgggtca cgatcgcgga
    21541 gccgtagaac atgaggtata ggcggtcaat caaccagtcc cagtatgtgt tcgacgtgac
    21601 gtctgcgtgt tcgtacgggt cggtgcggag gttgaacaac ttcggcgccc gcagcggagt
    21661 gaacggttcg aaccacacct gcagtgtgcc aggacagcgc tgctccatga aaacgatctt
    21721 ccaattctcc gcgcggatgc cgagcacgtc gcagtcgtcg gagaagtaga tcatcccgcg
    21781 ccgggggctt ttctcgacct cgccggtcag atagggcagt aggttgtacc cgtcgatgtg
    21841 gaccttgtat ttcatatcgc cggcctcgtg gcccttcttc agcttctcga cgatgtcggg
    21901 ctcgcctgcc gccgccagga acgttggcag ccaatcatga tgctgcacaa tctcgttgga
    21961 tacagagcgg ggcttgatct ttccggtcca gcggatcatc tccggtatcc ggaacgcgcc
    22021 ctcccagttg gtggctttct cgctgcggaa gggggtggtg gcgccgtctg gccagctatt
    22081 ggcatgcggg ccgttgtcgg tgctgtagat gacgatcgtg tcctcggcga tgcccagttc
    22141 gtcgaccagg tcgagtagct ggccgacgtt gcggtcgtgg tcgatcatcg tgtcgtggta
    22201 cggcgactgc cagcggccgg cctgcccctt gctctccggc ttggtgtgtg tccggaaatg
    22261 catgtgggtc atgttcatcc acacgaagaa aggggtttcg gcctcatgct ggcgtctgat
    22321 gaaatcgata cacgccgagg tggtttcgtc gtcgacggtc tccatccgct tctttgtcag
    22381 cggcccggtg tcctcgatgc gctgcttgcc gaccgggccg taccggtcgt ccacctcacc
    22441 ggtgtcctcg tcggtggccc aggaatggat cacgccacgc ggcagcaagg ccttgcgcag
    22501 caggggcgcc tcctcggcgg tcgggtagtc ctcgtgttcg ggttcttctt cggcgttgag
    22561 gtggtacagg ttgccaaaga actcgtcgaa cccatgcgcg gtcggcaggt acttgttcag
    22621 gtcgccgagg tggttcttgc cgaactgacc ggtggcatac cccagtggct tgagcagttc
    22681 ggcgatggtc gggtcttcct tctgcagtcc gacatccacg ccgggcatcc ccaccttgct
    22741 cattccggtg cggtacacgc tctgaccggt gatgaacgac gaccggcccg cggtgcagct
    22801 ctgctcgccg taggaatcgg tgaaaagcat tccctcgtcg gcgatccggt cgatattggg
    22861 cgtgaagtag cccatcatcc cgcggctgta acaactcagg ttcgagatgc cgatatcgtc
    22921 accccagatg acgagaatgt tcggcttctt atcgggcatt ttctaatcct tcgctaggaa
    22981 ctcgtgcaat ttctcgaccg cctggtcgat gctgaagctg gcgggtgggt gccgcggcgg
    23041 gaactccttg aacgtctcga ggaacttggt tgccatcgcc gtcgcataga agacgaaata
    23101 gtcccggtgc atgaaccagt cgtagtacgt gttggacgtg atgtcggcgt actcatacgg
    23161 gtcggtgcgc agattgaaca gcttgggtac ccgcagcggg gtgaacggct cggcccagat
    23221 tctcagcgtg ccctggcagc gctgctccgc gaacaccacc ttccagtttt cgaaacgcat
    23281 tgcgaccagg tcgccgtcgt cggagaagta gaagaagcct cgccgggggc tgtgttccac
    23341 ctcggccatc aggtagggga gcagattgaa gccgtcgatg tggaccttgt actccacgtc
    23401 accgatttgg tagcccttct tgagcttgtc catgacgtct gggtcgcctg ccgccgccag
    23461 cagcgtcggc agccagtcgt gatgctggat gatgtcattg gatacggtgc ccgccttgat
    23521 ctttcccggc cagcggatca attccggcac ccggaatgcg ccctcccagt tcgtgttctt
    23581 ctcgctgcgg aacggggtgg tacccgcgtc cggccagctg ttgcggtgtg ggccgttgtc
    23641 agtggagtag atgacgatcg tgtcgtcggc gatgccgagt tcgtcgagca catccaaaac
    23701 ggtgcccacg ttcttgtcgt ggtcgatcat cgcgtcgtgg tactccgatt gccacagtcc
    23761 ggcctggccg cggctctccg gcttgacgtg ggtgtagagg tgcatgtggg tgaagttgca
    23821 ccacacgaag aacggattgc ctgccgcatg ttggcgcttg atgtagtcaa tggtggcgtc
    23881 ggcgatatcg tcgtcgatgg tctccatccg cttcgtggtc agcggcccgg tgtcctcgat
    23941 ggtctgcttg ccgaccggtc cgtactttgg gtcgtcggct tcggacgaca cctcgtcggt
    24001 ggccttgcat ttcaacactc cgcgcgggtg cgccagttcg tagagcaatg ggaactggtc
    24061 cttgtgcggg tagtcgaact gttcgggctc ttcctcggca ttgaggtgat agaggttgcc
    24121 gaagaactcg tcgaatccgt gcacggtcgg taggtacttg ttcatgtcgc cgaagtggtt
    24181 cttgccgaac tggccggtgg catagcccag gggcttgagc agttcggcga tcgttgggtc
    24241 ctcggcggcc caaccaacat ccgcgccggg aatgccgacc ttgctcattc cggtgcggta
    24301 cacgctctgc ccgctgatga acgccgcacg cccggcggtg cagctctgct cgccgtagga
    24361 gtcggtaaaa cgcattcctt cgttggcgat ccggtcgatg ttgggggtgc gatagcccat
    24421 caggccgtcg ctgtagcaac tcaggttgct gatgccgatg tcgtctcccc agatgaccag
    24481 aatgttgggc ttaccgtttg gcatcggaag cctccttttc aattgacgtc cagtcggagc
    24541 ctaggggcgc aacgtcgttg cgaccccgca tttggccaac gattcagcgg caatacatct
    24601 ggtgcctagc cctcgctggg agcactctgg taaccggagg gtgatggcga tgtacgacaa
    24661 ggatctaagc aacaagtggc gggtcgaaat cgacttcgac gaagacgaga cccacacaca
    24721 tgcgacggtg cgcgcccagt gcggcgacgc cgagaccgtg acggcgctgg gcgacgccta
    24781 ccgcaacccg aaagactcca gccagccaat ggttggcgag gagatcgccg cggcacgcgg
    24841 gctcatcgcg ctgggcacgg agctgctgca acgcgcgtcg tcgcagatcg agcaggccac
    24901 gcaacagccg gtccacttgt atcgctgact cgtggccctg gccgtctgcc tgctgttcga
    24961 cccgaaatcc gagcgggcgg tccgcgcctt gtgggacagg gtcgaagagc agggtgtcgg
    25021 cagcctgcgc tcgcacactc acggcagtca tgtgccgcat gtgtcctacg cggtgctgcg
    25081 caagtgggac caagaggcgg tgacggatgc gctggccggc ctcgaccccg gcgaacccgt
    25141 gaacctcagc ttcgacggcg tcgggctgtt ccgacgcggc cgcacttggc tggtcgccgg
    25201 tgtcaccgcg gacttcgtgg cccgccagca gcgggtcgtc gaggcggtca ccgccactgg
    25261 cgccgaactg cacaagcact accggccggg aatctggttg ccgcactgct cgctggcgcc
    25321 ccgggccaca ctggctcagt tgccgcagct ggtggctgcg gtgatggatg tgctgccgct
    25381 gccggcccga ctggaccggg ccgcattggt caacagcgcg acgggggaga tcaaccctct
    25441 agcggagatg ccgtaaccac gctgaccgca ggctcgtggg cctcgccctt catctggtga
    25501 aagaggtcga cgtagtacgg aagacagtcg gccatcgcct gttcggtggt gaacagcggc
    25561 tggtaaccga gatcgcgctg cgccttggcg atcgagaagt agttgtcgag gtaaagccgt
    25621 tccaccccaa ggggttccag cagcggcttg ggcaggccga agcgaaagtg gaaccactgc
    25681 cacaccatca tcacgacttt caccaggcgt cccggcaccc agagcttcgg ccacggctgc
    25741 ccgcatgcat cgaccacggg ttgggagaac tcgaacatgt tgatcggttc gccgtcgttg
    25801 atgaagtacg cctggccggg tgccgttcca ccgggcacca gatgctccgc ggccaggatg
    25861 aatccgtgaa tcaggttgtg tacgtaggag ttatcgagct tgaccttctt gctgccgacc
    25921 agcaccttga cgtggccggc gagcacgttc tcgaacacct tgcggaacat ggtctgatcg
    25981 ccgcggcccc agatcccgct cggtcgaatg gagcacgtca gtaggccatc aataccgttc
    26041 tgcgacaaga caaactgctc ggcgacgacc ttggtctcgg tgtagaggtc gttgaagcgt
    26101 tcggtgtagg gcagggtctc atccccgccg gagatgcgct tgccgcccat caccacactg
    26161 ttggatgcgg tgtagacgaa ccgctttgtg ccggccgcct gcgcggcgtg cacgaggttc
    26221 ttggtgccct cgacgttgac cgcgaagctg cgctgccggt actcctcggt cactgacgcg
    26281 ccgcccatca ggtcgatgat cgccgcggtg tggaagatcg tgtcgatgcc ctcgacggcg
    26341 gcggccacgg tctgctggtc gcagatgtcg cccgtaagca cctcgagccg cggatgcccg
    26401 gcgagtgacg acggggcccg gtcgaaggag cggacgtggt ggccgcggtc cagcagttcg
    26461 gtcaccaggt tggcgccgac gaagccggag cctccggtaa ccagcacgcg gcccaactcg
    26521 gtggtcaacg ttgaatcacc catgccgcag agcataaatg aaacgtgtta cagtttcgag
    26581 cccctatgcg gaaaatggtt acgaactcaa ttatcgtcgt cgtctttgga ggccagcgca
    26641 tcctcgacgc gcttgcgtgc tccagctaag tgttcttcgc agcgtttagc cagttcctca
    26701 ccgcgttccc agagtttgag cgacgcatcc aggtccagac cgccctgctc cagctgctgc
    26761 acgacatcga tcagttcgtc gcgggcttct tcgtacccca gctgactaat aggcttcatt
    26821 ccttgccctt gcttacggcg gtaacggccc cgtcggcgac ccgaacccgc aaccgtgtgc
    26881 ctgacggagc gtcggccgtc gtgcgcagaa ttgcgccgga agccgtctgg acgacggcgt
    26941 agccccggtc cagggtggcc gccggtccca gtgtggtgag ccgggccgac aagtgcccga
    27001 tccggtcgga ttcggcggcc accaaccggg tgatgtcacg tcgaacgcag gcgcgcgctc
    27061 ggtggatctc gtcggcccgc gcggtgagcg cggccagcgg ttgggccagc accggcctgc
    27121 tccgcagttg cgacaacgtg tgctgctcgc ggtgcaccca gttgcgcagc gcccgtgcgc
    27181 agcgccgccg cagatcggtg atcaacgcct gctcggccgc ggcgtcgggc acgatccgct
    27241 tggccgcatc ggtcggagtt gccgcgcgga cgtcggcgac gaggtcgcac agcgggttat
    27301 cgggttcgtg gccgaccgcg ctgacgaccg gtgtggtgca tgcggcgata gcgcggcaca
    27361 acgtctcgtc ggaaaacggc agcaggtcct cgacgctgcc accgccgcgg gcgatgacga
    27421 tgacgtcgac ggcggcatca ccatcaagct cgcgcagcgc gtcgaggatc tgcggcaccg
    27481 cgttggggcc ctgcaccacg gtgttgcgca ccgcgaagcg caccgcgggc cagcgaccgg
    27541 tggcgacggt catcacatca cgctcggccg ccgaggcgcg gccggtgatc aacccgatgg
    27601 tcccgggcag gaagggaagc ggccgcttga gccgcggatc gaatagcccc tccgcgtaca
    27661 gcagctggcg cagccgttcg atgcgggcca gcagttcgcc gacgccgacg gcgcgaatct
    27721 cgctgactcg caaccagaat gagccacggc cggtgtggaa ataaggcttg gcgcacacca
    27781 ccacctgggt gccctcggac agcttgaccg gcgcgttgtg caccagatcg cgcggacagg
    27841 tcagcgagag tgacatatcg gcggccggat cccgcaacac catgaacacg gtcttcgaat
    27901 ccgcccgcac cttgagttca gtcagctggc cctcaaccca caccgcgccg agtcggtcga
    27961 tccacttggc gacccgcgtg gccaccgcgc gcaccggcca cgggttctcc ggggactggc
    28021 ctggctcggg agcggtcact tggcggtcgc gcgggtgatc ctgttggaca gcagggtctg
    28081 gaacggggcg cgcgccttgg tggcgtcttc gtaggccagc agtgcctcga ggtcggcgac
    28141 gcgcagcgac gtcaaccgag cccgcagctg ggccagcgtg agcgactcgt aatcgagttc
    28201 ggcgacgacg tcaggcgagt cggcggccgt cgacgcgtta gacggcttgg cgtcagcgcc
    28261 gtcgggttcc ccgtcggtga acagcgcaaa tcggccctcg gtcaggcgtt cgccgtcact
    28321 gctcggcgtg gccagcccgt tgtcgtcgag gtcctcgtcg aacgtggccc actccggttg
    28381 ctcgtctttg ggcgggaaga gttgatcaag cgcctcgtca cccctgatga ccaggtcggc
    28441 gacgtcctgc tgcaccttca tcacgaggtg ggcgatctgg ctggcgacgg tcatcgggta
    28501 catcaggatg gtctgcggaa gctttcgggt ttcctcgaag gcggtcaccg ccgcacctac
    28561 cagcagacgg accccatacg gtgcagttgc catggcccca agactacggg cgatcgtctg
    28621 gctggcctcg gtaggtcgag catcagtacg cttggcccca tgccgccgac gataaatatg
    28681 gggattccgg gtgcctcgag ctcggtgacc ggtggcgtta ccggcaagcg ggtgctgctt
    28741 gccgagcctc gcgggtattg cgcgggcgtc gatcgcgccg tcgagaccgt ggagcgcgcg
    28801 ctggagaagc acggcgcccc cgtgtacgtg cgccacgaga tcgtgcacaa ccgccacgtc
    28861 gtcgacacgc tggccaaggc cggcgcggtg ttcgtcgacg agaccgacga ggtgcccgag
    28921 ggtgcgatcg tggtgttctc cgcccacggg gtggcgccga cggtgcacgt caacgccaaa
    28981 gagcgcagcc tgcaggtcat cgacgccacc tgcccgctgg tcaccaaggt gcacaacgag
    29041 gccaaacgct tcgcccgcga cgactacgac atcctgctga tcggccacga ggggcacgag
    29101 gaggtcatcg gcaccgcagg cgaggccccc gaccacgtgc agctggtcga cgggccggac
    29161 gccgtcgaca acgtgaccgt gcgcgacgag gacaaggtga tctggctgtc gcagaccacg
    29221 ttgtccgtcg acgagacgat ggagaccgtg cagcggttgc ggcagcgctt ccccaagctg
    29281 caggacccgc ccagcgacga catctgctac gcgacgcaga accgtcaggt cgcggtcaag
    29341 gcgatggccc ccgagtgcca gctggtgatc gtggtcggct cgcgcaactc gtcgaattcg
    29401 gtgcggctgg ttgaggtggc gcttggcgcc ggttcggacg ccgcccatct ggtcgactac
    29461 gccgacgaca tcgacccggc ctggctttcg ggggtgacga cggtcggtgt gacgtcggga
    29521 gcgtcggtgc ccgagatctt ggtgcgcggt gtgctcgagc ggctggccga ctacggctac
    29581 gacgtcgtgc agccggttac caccgcgaac gagacgctgg tgttcgcgct gccccgggag
    29641 atccgcccgg cgcgcgcgtg acgtttcccg cgcgaccaga cgcaaaagtg cccaaaatcc
    29701 gcgtcagaag ggcacttttg tgtctgctca cgcttgtctt ggtgctggct ggttgcggcg
    29761 acgacgcgac ggatcggctc aacgccgtgg tgcatacgtt cgccgactcc ctcaacaacg
    29821 gtgacgcgca ggcggccgcc gccgtcacga ccgatcccgc gcaagcatcc gacaccctcg
    29881 gaaaactgtt cgacagcctc ggccgcaacg cccacttcga agtctcgacc gtcgaccgca
    29941 aagacaacga cgcgacgttc ggcctttcgg ccacatggaa attcggcaag gacggccgca
    30001 accagtggac ctacaccacc accggcagcg cgggccagag cggcgatcag tggaagatcc
    30061 agtggaatcc ggcgaccgtc gcgccgggcc tcggcaccgg cccgctgtcg tacagcgcgc
    30121 tgacccccga ccccgccgcc agggtactgg accgcgcggg cgccgagctg ctgacccagc
    30181 aggttgtcac gctggtcaac ctgtcacccg gtgccgacgt gaatgccgtt gcggcgctgc
    30241 tcaatccgat cgacgcgggc atcaccgcgg agtcgctgca gacgcaattg aacgcggcca
    30301 acggtcagca ggtcaccgca gtcacgctgc gggcggagga tctggcaccc atcgaggagg
    30361 ggctgtcggc cctgccgaac gtgacgctgg cgccgcagac gcggcttttg agcaccgaca
    30421 aggcgctgac atcaccgacg ctgtccgggc tgaccgacct gtggcaacgc gccaccgatg
    30481 acgccggcgg atgggcggtg agcacccaga ccgctagcgg gagccagcgc gtcggcgggc
    30541 aggaccccaa acctgtctcc gacatcgcca gcacgctcga catcggcatg acgcgcgccg
    30601 ccagggatgc gttagccccg atctccaccc ctgccgcgat ggtggtcatc caaccctcca
    30661 ccggcgacgt gttggccatc ggccagaacg ccgccgcgga tgctcagggt ccgatcgcgc
    30721 tgacggggct gtatccgccg ggctcgacct tcaagacggt gacggtctcg gccgcccttc
    30781 aggccggcca ggtgacaccg gacagcatcg tggcgtgccc gggcacggag aacatcgagg
    30841 gtcgccagat tcccaacgac gacaacttcg acctcggcca ggtgccgctg cacaccgcgt
    30901 tcgcgcggtc atgcaacacc acgatgggcc ggctggcggt caatctgccg cccgacggcc
    30961 tcaccaaggc cgccgcccaa ctgggtctgg gcatcgacta caccgcaccc ggcatgacca
    31021 ccgtcaccgg caaggtgccc gtcgccgaca cctcggcgct gcgcgtcgag gagggcatcg
    31081 gccagggcca ggtcaccgca tcgccgttcg gcatggcgct ggtagccgcc gcgctggcgc
    31141 acgggtcggt gccgtcgccg acgatcgtcg ccggccagcc cggcaagccc gaccataccc
    31201 ctgatccgct gccggcaagc atcgatgatc aagtgaaatc gatgatgcgc gagaccatta
    31261 cgagtggcac cgccacccag ctccaagaca ttcccggcgt gctcggcaag accggcacgg
    31321 ccgagtacat cgacaacact cacgcacacg gctggtttgt cggcatcgac ggcgatctcg
    31381 ccctggcggt attcgtcagt gacgcgggca gttccggccc ggcggcggac gccgcgggtc
    31441 gattcctgcg cgcgcagcat ccgtgagttt gcaaatcaac atcaaagtcc gctacccgcc
    31501 tggtctttgc agatacgatc gcgttgacgg gaggggaggc cgaaagtggg gtctccgagc
    31561 agtgtggaca caccgccgtc ggcggcttct gagtcgtgga tgcgacggct tgcccgtacg
    31621 acgcctggcg tcatcggcat catcgcgctc gctgtcgcgg caatttgcgt gattgccggc
    31681 gtggtgtgcg ggggtcagct cgatgggcgg atcgacgaac gcaacgcggt tctgacgagc
    31741 tctgaaccgt tcgcctatgc ggcgcagaac ctctacgccg ccctttcggc cgccgacgcc
    31801 gctgcagcgt ccgcgttcct ttctggcgga atcgaaacgg cagagatgcg tgcgaagtat
    31861 cagcgtgcgt tggctgacgc ggcatcggca ttggccgaca ccaccgcggg gactactgat
    31921 ccggcaaccc gaacctcgct cgcggacata tcggcgggat tgtctgccta caccgccctt
    31981 atcgagtcgg cgcgggcgaa caaccggcag ggcttcgtga tcggctccgc ctacttgcgc
    32041 gaagcctcga cgttgatgca gacatcgctg cttcccggcg ccgagaagat cttcaccgcc
    32101 aatctcgcca cggtcgacaa agaccaacgc gccgtcggtt cattgcccat cgccagcctc
    32161 gtgctcctga cgctggcgct cgtcgcgatc ggtgtcgctt cgatgatcat gtacggccgc
    32221 accaaccggc agttcaacat cgggctcgtg atcgccgcgg ccaccgtggt gctggtgatt
    32281 ggctgggtcg tcgtggccac tcagttggcg gcgggcgaga tcgagcacag ccgagccgaa
    32341 ggcaccgcga ggttcgaaca gctcgcgaag gcgcgcatta acgcccagca ggcgcggaca
    32401 gtcgagaccc tcgagttgat cgcgcggggt gacatcactg ccagagagaa gtcgtttcac
    32461 ggccacatcg acgagctggg caagctgctc gacgcggcgc cggcggcca
//
LOCUS       DASRJA010000451         7799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_407475,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000451 DASRJA010000000
VERSION     DASRJA010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407475"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="VFC01_19875"
     CDS             complement(<1..1393)
                     /locus_tag="VFC01_19875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN81923.1"
                     /translation="MAQPTDQAHAADGFDDAVDQLRADYDRTPYTSVSFPPSAPGQLA
                     AVAHLFGLATPELSTARVLEIGCATGGNLIPFAAAHPEARVVGVDLSQVQIDLAHTRV
                     QDLALNNVDFVAGDIARMDLTALGQFDFIIAHGVFSWVPPEVQEALLSALRRLLVPEG
                     VAYLSYNVYPGWKSKEVLRDAMQLASGGSASPEDKVREARGIVDFLEEVVPADGVLAR
                     VLAEFRADSEGYGDAYLLHDELETFNSPCYFYEMLARARAHGLAFLAEARPEAMFPGN
                     YGPKVEEHLREKCGGVQVLVEQYIDFVVNRTFRESLLIHAEREPQIRYHPDPNRFRDV
                     HIAAWVPPADEPTRLDSSRQEYREADGATLFTNDPGIKAALDALSARWPWTLSRHELV
                     DAVHARLVDAGLTPSDNLPDIIDNLMGVLILQGQARYRLHPVSPEPVSAPLRLDETAR
                     RMVELTATDGQAST"
     gene            complement(1457..2875)
                     /gene="proS"
                     /locus_tag="VFC01_19880"
     CDS             complement(1457..2875)
                     /gene="proS"
                     /locus_tag="VFC01_19880"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZN81924.1"
                     /translation="MGNQTAVLTPQHEDFPRWYQGVLTKAKLAENGPARGTMVIRPTG
                     YAIWERMQAEIDARIKVAGAQNAYFPLLIPMSFFEREAEHVEGFSPELAVVTHGGGKE
                     LTEPLAIRPTSEAVIGEYMAKWVDSYRDLPLLLNQWSNVVRWELRPRIFLRTTEFLWQ
                     EGHTAHVTESDARKYARRILHEVYQDFMRSVLAIPVIVGSKTASERFAGAENTMTCEG
                     IMRDGKALQMGTSHEFGQRFSRAFNITYSSAEGATELCWTTSWGASTRMLGGLIMTHG
                     DDFGIVVPPALAPVQVVVVVVKDTDGAVTRTASALAQDLKGRGVRVKLDDNIDQAFGW
                     RATEWDLQGVPIRVELGPRDLADNVALLYRRDTRDKTQVGIDNLVAEVQQLTGRIQTD
                     MFEAATARRDALVTDCATLDEAREAAQTGVARLPWELVGTSGEQDFATSGVTVRCLQL
                     SDGSLPDTDHDRGALAYVARAY"
     gene            complement(2887..3609)
                     /locus_tag="VFC01_19885"
     CDS             complement(2887..3609)
                     /locus_tag="VFC01_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81925.1"
                     /translation="MARTSPSGDEVQLAADHVWGTVAHLERRIEARFGERGLTKVARD
                     LGGLVHLVQSEADQSRDRLRRTTLAARGAGILIVAATLVALVFSLRSVVIDGLTRTAD
                     WVPLVESVINDLVFAAIAVVFLWAFPERLERRTMLRLLHRLRSLAHVIDMHQLSKDPE
                     QLSPTYTPTAQSIVHGLDADQLYHYLTYCSEMLSLTAKTSALCGEYSTDGVVLENIAD
                     IETLTTELSNKIWQKISLLPRP"
     gene            3669..4448
                     /locus_tag="VFC01_19890"
     CDS             3669..4448
                     /locus_tag="VFC01_19890"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HZN81926.1"
                     /translation="MLRGLNWFLFALAIIVAGGVTAAPANADPNALWTIVHDQCVPDQ
                     QTSGDPAPCSLVDLGAAERQGYAVLKDLVGATQFLVIPTGRISGIESPTLLEPGVTDY
                     FAAAWNARGFVEARAGVDIPRDWMSLAVNSAVARSQDQLHIHIDCVRADVRDAVSRHI
                     ADVGSDWVPFPEPLRGHPYLAMTVEGDDLNATNPVRLVAQRVDDMGIATIVVVGTYLK
                     NGQPGFILLAGQADPAIGDRGAGEELQDHMSCPPPRGQWAK"
     gene            4539..4718
                     /locus_tag="VFC01_19895"
     CDS             4539..4718
                     /locus_tag="VFC01_19895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81927.1"
                     /translation="MMWKIFAAVCASGLIIAGAAALAFAPADSGPSLSNSTTTPTTTT
                     TSATMPNSPGYMGGY"
     gene            complement(4751..5245)
                     /locus_tag="VFC01_19900"
     CDS             complement(4751..5245)
                     /locus_tag="VFC01_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN81928.1"
                     /translation="MQSYTVRFHIDAPPRKVWRVLHPPVPPDSPRPRVLKWPTGSMEI
                     LNEGDEAGEGLVRTCIFEVPKYLLSGGKGRSWETVTEAKLNKLSRYVAVGKPLWSRAE
                     GYHELEEQPDGTTVLTFHETYYAYNPILRFLLERPVHAKISRDNLKTYEHALGYAGRV
                     TRVT"
     gene            complement(5245..5874)
                     /locus_tag="VFC01_19905"
     CDS             complement(5245..5874)
                     /locus_tag="VFC01_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZN81929.1"
                     /translation="MDISSVDELLTTTRSVRKRLDLTRPVDRKIILECVRLAMQAPTA
                     SNAQDWRWVVVTDADKRAAIAEIYRSVGAEYLAHAASAESDPQTRRVYESALGLTETL
                     AKVPVHVIPCLESRFDGSDQLVAASAWASIIPAGWSFLLALRSRGLGSVWTTMHLAKE
                     REVGELLAIPATATQAALFPVAYTIGTDFRPAARPPAETITFWNTWGER"
     gene            complement(5878..6360)
                     /locus_tag="VFC01_19910"
     CDS             complement(5878..6360)
                     /locus_tag="VFC01_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN81930.1"
                     /translation="MARLDVPDGPGGEAAMIWALRPQLGGMVERMIRGAYQESILPAA
                     ERELARMRIAQINDCVACSDFRAPSVLDAGIAPELYENVAAYATYPGYTARQRLAIEY
                     AERFATAHASIDDTFFGRLRELFSDAEILDLTLCIAVFLGLGRSLTVLGVDQSCAIDI
                     "
     gene            6430..7158
                     /locus_tag="VFC01_19915"
     CDS             6430..7158
                     /locus_tag="VFC01_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81931.1"
                     /translation="MQRLRMGGLGVLFMHEVHTVRGRAEDEFEAAFRDGWMPMLAAGD
                     DARLLWYTNHAHGSGPAYTVVTVTALRDGTAWEHLALRIQKGDLRTWMRDLDELRYDV
                     DAKLLVPLAWSPLKEVDFGEVPTDGREHELTLYMEDTMWPYEDKLGEYVDRCGEVYSK
                     SLDHPNPLLTIEAAFQPALGSHRRREVALMQRIHDSQALLRLLQTELPPEYRSPGTWM
                     HDALELRDQWTSRLLRTSAWSPMY"
     gene            7167..>7799
                     /locus_tag="VFC01_19920"
     CDS             7167..>7799
                     /locus_tag="VFC01_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZN81932.1"
                     /translation="MNIGTLVDAPAAGDPDRAALIIDGRVISYGELATAVERCAARLS
                     DSEPAGARIAVVDVGNLASIATMFGAARIGAAAALMNPALTPPELLALVKAAGCHPVG
                     VAGAEYVDRLLDAALPAVWTATDLIDDDGAPPAPPPADDVDDREALVLFTSGTTGLPK
                     PIGITHRRLSARIRGVTTPFRADVPPVVSMMSVPFFHVGGAIGVLGNLYSG"
BASE COUNT         1388 a         2559 c         2496 g         1356 t
ORIGIN      
        1 aggtcgacgc ctggccgtca gtcgcagtca gctccaccat ccgcctggcc gtctcgtcga
       61 gccgcagcgg tgcagacacc ggctccggcg acaccggatg cagccggtat cgcgcctgcc
      121 cctgcaggat cagcacgccc atcagattgt cgatgatgtc tgggaggttg tcgctgggcg
      181 tgaggcccgc gtcaacaagc cgggcgtgca ccgcatcgac cagctcgtgt cgtgacagcg
      241 tccacggcca tcgggcactg agcgcgtcaa gcgcggcctt gatgcccgga tcgttggtga
      301 acagggtcgc gccgtcggcc tctcggtact cctgacgtga gctgtcgagg cgagtcggct
      361 cgtcggcggg aggcacccag gcggcgatat gcacatcgcg aaatcggttg gggtccgggt
      421 ggtatcgaat ctgcggctct cgttcggcat ggatcaacag cgattcgcgg aatgtgcggt
      481 tgactacgaa atcgatgtat tgctccacca gcacttggac gccgccacat ttctcacgca
      541 agtgctcttc gactttgggg ccgtagttgc cggggaacat tgcctcgggg cgggcttccg
      601 cgagaaacgc cagaccatgt gcgcgggcac gcgccagcat ttcgtagaaa tagcagggcg
      661 agttgaacgt ctcgagttcg tcgtgcaaca ggtaggcgtc accgtaaccc tctgagtcgg
      721 cccggaattc ggccagtact cgggcgagca cgccgtctgc ggggaccacc tcctcgagga
      781 agtcgacgat tccgcgcgcc tcgcgcactt tgtcctcagg gctcgcgcta ccgccactgg
      841 ccaactgcat ggcgtcgcga agaacttcct tggacttcca ccccgggtac acgttgtagc
      901 tcaggtaggc caccccctcg gggaccagca gcctgcgcag cgccgacaag agcgcttcct
      961 gcacctcggg tggcacccag ctgaatacgc cgtgggcaat gatgaagtcg aattggccca
     1021 acgccgtgag gtccattcgc gcgatgtcac cggcgacaaa atcgacattg ttcaaggcca
     1081 gatcctgtac ccgtgtatgc gcgagatcaa tctgcacctg cgaaagatcg acgccgacga
     1141 cgcgcgcctc cggatgcgca gcggcaaacg gaatcaggtt gcctccagtg gcacaaccga
     1201 tttcgagcac acgcgcagtg gatagctcgg gcgtcgccaa cccgaacaaa tgggccaccg
     1261 cagccagttg acccggcgcc gacggcggga acgacaccga ggtgtatggc gtcctgtcgt
     1321 agtcggcgcg cagctggtcc accgcgtcgt cgaagccgtc ggcggcgtgc gcctggtcag
     1381 tcggttgagc cacttccgct ccctgtctaa gtggtcgacg ttgaccgacg ctgttagtca
     1441 atattcgatc acacgctcag taggcgcgtg cgacgtaggc cagcgcacct cggtcgtgat
     1501 cggtgtcggg cagtgagccg tcgctgagct gcagacaccg cacagtgacg ccgctggtgg
     1561 cgaagtcctg ttcgccagag gtgccgacca actcccacgg caagcgagcg acgcccgtct
     1621 gcgctgcctc ccgcgcctcg tcgagggtgg cgcaatcggt gaccagggca tcgcggcgcg
     1681 cggttgccgc ctcgaacatg tccgtctgga tccggcccgt cagctgctgc acctcggcaa
     1741 cgagattgtc gatgcctact tgggtcttgt cgcgggtgtc gcgccggtag agcaaggcaa
     1801 cgttgtccgc gagatcgcgc gggccgagct ccacgcgaat cggcacgccc tgcaggtccc
     1861 actcggtggc ccgccagccg aacgcctggt cgatgttgtc gtccagcttt actcgcacgc
     1921 cgcgcccttt gagatcctga gctagggcgg aggcggtgcg ggtgacggca ccgtcggtgt
     1981 ccttgaccac cacaacgaca acttgcaccg gtgccagcgc cggcggcaca acgatcccaa
     2041 agtcatcgcc atgggtcatg atcagtccgc cgagcattcg cgtcgacgcg ccccacgagg
     2101 tggtccagca cagttcggtc gcgccctcgg cgctggaata ggtgatgttg aacgcgcggc
     2161 tgaacctttg gccaaactcg tgcgacgtgc ccatctgcag cgccttgcca tcacgcatga
     2221 tgccctcaca ggtcatggtg ttctccgccc cggcgaaccg ttcgctggcg gtcttgctgc
     2281 caacgatcac cggaatggcc agcacgctgc gcatgaaatc ctggtacacc tcgtgcagga
     2341 tgcggcgcgc atatttccgc gcatcggatt ccgtgacgtg cgcggtgtgg ccttcctgcc
     2401 acagaaactc cgtggtgcgt aggaagattc gtggccgtag ctcccaccgg acgacgttgg
     2461 accactggtt gagaagcagc ggcagatcgc ggtagctatc gacccatttg gccatgtatt
     2521 cgccgatgac cgcttcactc gtcggacgga tcgccagcgg ttcggtcagt tccttgccgc
     2581 cgccgtgcgt cacgacggcc agctcaggac tgaatccctc gacgtgctcg gcctcgcgtt
     2641 cgaagaagga catcgggatc agcagcggga agtacgcgtt ctgtgccccg gcaaccttga
     2701 tccgcgcatc gatctccgcc tgcatccgct cccagatcgc gtagccggtg ggccggatga
     2761 ccatggtgcc gcgtgctgga ccgttctccg ccagcttcgc cttggtcagc acaccttggt
     2821 accaccgcgg gaagtcctca tgttgaggag tcagcactgc cgtctggttg cccatacccg
     2881 ctaagcctag gggcggggga gaagcgaaat tttctgccag atcttgttgg aaagctcggt
     2941 ggtgagagtc tcgatgtcgg caatgttctc gagcacgacg ccatcggtgc tgtactcgcc
     3001 gcacaacgcc gacgtcttcg cggtgaggct caacatctcc gaacagtagg tgaggtagtg
     3061 gtagagctgg tccgcgtcca ggccgtgcac gatgctctgt gcggtcggcg tgtaggtcgg
     3121 gctcagctgc tccggatcct tggagagctg atgcatgtcg atcacgtggg ccagcgagcg
     3181 gagccggtga agcagccgta gcatcgtgcg ccgctccaag cgttcgggaa aggcccacag
     3241 aaacaccacg gcgatcgcgg cgaacacaag gtcgttgatg acgctttcga cgaggggcac
     3301 ccagtcggca gtccgagtta gcccgtcgat gacgaccgag cgcaggctga agaccagtgc
     3361 gacgagtgtc gccgcgacga tcaagatgcc cgccccgcgc gctgccagcg tcgtacggcg
     3421 aaggcgatcg cgagactggt cggcctcgct ctggaccaga tgtacgaggc cccccaaatc
     3481 gcgcgccacc ttcgtcagtc cgcgttcacc gaagcgcgcc tcgatgcggc gttcaaggtg
     3541 cgcgacagtg ccccagacgt ggtcagcggc cagctggacc tcgtcaccgg aggggctcgt
     3601 acgtgccacg tgatgactgt aggggtgtcg gcggcgccag cgcccgaaga ctccgcaagg
     3661 atctacatgt gcttcgagga ctcaactggt tcctgttcgc actggcgatc attgtggccg
     3721 ggggagtgac agccgcgccg gccaatgccg atcccaacgc gctatggacg atcgtgcatg
     3781 accagtgcgt gcccgatcag cagaccagcg gtgaccccgc cccatgctcg ctggtcgacc
     3841 tcggcgcagc agagcggcaa gggtacgccg tgctcaaaga ccttgttggt gcgacgcagt
     3901 ttctcgtcat acccacgggg cggatctccg gcatcgagag cccaacgctt ctcgagccgg
     3961 gtgtcaccga ctacttcgcg gcagcttgga acgcaagggg tttcgtcgag gcgcgggccg
     4021 gcgtcgacat cccacgtgac tggatgagtc ttgcggtcaa ctccgccgtg gcgcgatcgc
     4081 aagaccagct gcacatccac attgactgcg tccgcgctga cgtccgcgac gctgtgagcc
     4141 ggcacatcgc cgatgtcgga tcggactggg tgcccttccc agaaccactg aggggacacc
     4201 cttatctcgc catgaccgtc gagggcgacg acctcaacgc gaccaaccct gttcggctcg
     4261 ttgcgcagcg agtcgacgat atgggcatcg cgacgatcgt ggttgtcggc acgtatctga
     4321 agaacggaca gccagggttc atcctgctgg ccggtcaggc tgatccggcc atcggcgacc
     4381 gcggcgccgg tgaagaactg caggaccaca tgagctgccc accgccacgc gggcaatggg
     4441 cgaagtagtc cacgacggtc actgaacctt gcgacgccgt cgatcgtgca taacgtgcag
     4501 ggtccgtcac tgccgcggac ccgggacgga gttcatcaat gatgtggaag atctttgcag
     4561 cagtctgtgc gtcgggcctg atcattgccg gagccgcggc gctcgcgttc gcgccggccg
     4621 attcgggacc gtcgctgtcg aactcaacga cgacgccgac cacaaccacg acgtcagcaa
     4681 caatgccgaa ttcgcccggc tacatgggcg gctactgaag aagatcctgc tgcggccaag
     4741 gcaatagcaa tcaagtcacg cgtgtgacgc ggccggcgta gcccagcgca tgctcgtacg
     4801 tcttgaggtt gtcgcgagaa atcttcgcgt gcacagggcg ttccagaagg aaccgcagta
     4861 tcgggttgta tgcgtaatac gtctcgtgga acgtgagaac cgttgtgccg tcaggctgtt
     4921 cctcgagctc gtggtagccc tccgcccgag accagagcgg cttgcccacc gcgacataac
     4981 gcgacagctt gttcagcttc gcctcggtga ccgtctccca ggagcgtccc ttgccgcccg
     5041 acagaagata cttgggcacc tcgaaaatgc aggtgcgcac caggccttcg ccggcttcgt
     5101 cgccctcgtt gaggatctcc atgctgcccg tcggccactt gaggactcgc ggtctcggcg
     5161 aatccggcgg cacgggcgga tgcagcaccc gccacacctt cctcggcggt gcatcgatat
     5221 ggaatcgcac tgtgtacgac tgcattagcg ctctccccag gtgttccaga acgtgatggt
     5281 ctccgcgggc ggccgcgcgg ccgggcggaa gtcagtgccg atcgtgtaag caaccgggaa
     5341 cagcgccgcc tgggtcgcag tggcggggat tgccagcagc tctccgacct cgcgctcctt
     5401 ggcgaggtgc atcgtcgtcc agaccgatcc cagaccgcgg gaccgcagcg cgagcaggaa
     5461 actccagccg gcgggaatga tcgacgccca cgccgaggcg gcgaccaact gatcggagcc
     5521 gtcgaaacgg ctttccagac aagggattac gtggactggg accttggcca gtgtctccgt
     5581 aagcccaagg gcactttcgt acaccctccg cgtctggggg tcggactcag ccgaggcggc
     5641 gtgggccaag tactcggcac cgacgctgcg gtagatctcg gcaattgcgg cgcgcttgtc
     5701 ggcatcggtg accaccaccc aacgccagtc ctgcgcgttg ctcgcggtgg gcgcctgcat
     5761 cgcgagccgc acgcactcca gaatgatctt gcggtcgacc ggccgcgtca ggtcgaggcg
     5821 cttgcgcacc gaccgcgtgg tggtgagcag ttcgtcgacc gaagaaatgt ccaccggtca
     5881 aatatcaatc gcacaggatt gatctacgcc cagcacggtc aacgaccgcc cgaggccgag
     5941 gaagacggcg atgcacaagg tcaggtcgag gatctccgca tcgctgaaca attcgcgcag
     6001 ccgtccaaaa aaggtgtcgt cgatggacgc gtgcgcggtg gcgaaacgct cggcgtactc
     6061 gatcgcaagg cgctgccgcg ccgtgtagcc gggataggtg gcatacgccg cgacgttctc
     6121 gtagagctcc ggcgcgatgc ccgcgtccag caccgatggc gcgcgaaagt ccgagcacgc
     6181 gacgcaatcg ttgatttggg cgatgcgcat cctggccagc tcgcgctctg ccgccggaag
     6241 gatgctttcc tggtaagcac cgcggatcat gcgttcgacc atgccgccga gctgcggccg
     6301 cagcgcccag atcatcgcgg cctcgccgcc ggggccgtcc ggcacatcca gccttgccat
     6361 gatcgaactc cctgcatgtg agggtcgtta cccttgtgag gaaaccagat tctcggcgtc
     6421 gctgtccaga tgcagcgcct gcgcatggga ggtctcggcg tgctgttcat gcacgaggtg
     6481 cacacggtgc gcggacgcgc cgaagacgag ttcgaagccg cgttccgaga cggctggatg
     6541 cccatgctcg ctgcaggcga cgacgcacgc ttgctgtggt acaccaacca cgcacacggc
     6601 agcggccccg cctacaccgt cgtcacggtg accgcactgc gcgacggaac ggcctgggaa
     6661 cacctggccc ttcgcatcca aaagggcgat ctccggacct ggatgcgcga cctcgatgag
     6721 ctccgctatg acgtcgacgc caaattgctt gtgccgcttg catggtcgcc gctgaaggag
     6781 gtggacttcg gcgaggtgcc gacggacggt agggaacacg agctcaccct ctatatggag
     6841 gacaccatgt ggccgtacga agacaagctc ggtgagtacg tcgaccgctg cggcgaggtc
     6901 tactccaaga gcctggacca ccccaacccg ctgctcacca tcgaggccgc ctttcagccg
     6961 gcgctcggca gccatcgccg ccgcgaagtg gcactgatgc agcgcatcca cgactcgcag
     7021 gcacttctca gactcctcca gaccgagctg ccgcccgagt accgcagtcc cggcacctgg
     7081 atgcacgacg cgcttgagct gcgcgatcag tggaccagcc ggctgctgcg cacctccgca
     7141 tggtcgccca tgtactagag gagcacatga acatcggaac actcgtcgac gcgcccgcag
     7201 caggggatcc cgaccgcgcc gcgctcatca tcgatgggcg cgtcatcagc tacggcgaat
     7261 tggcgacggc cgtcgaacgc tgcgccgcgc gcctgtccga cagcgaaccg gccggcgcac
     7321 gcatcgccgt cgtcgatgtt ggcaacctgg cgtcgatcgc gaccatgttc ggtgccgccc
     7381 ggatcggcgc cgccgcggca ctgatgaatc ccgccctgac accaccggag ttgctggcgc
     7441 tcgttaaggc cgccgggtgt cacccggtgg gcgtcgcggg agccgaatac gttgaccgcc
     7501 tccttgacgc tgccctaccc gcggtatgga cggccaccga tctgatcgat gacgacggtg
     7561 cgccgcctgc gccgccgccc gctgatgacg tcgacgaccg cgaagcgctg gtgctgttta
     7621 ccagcggcac taccgggctc ccgaagccga tcggcataac ccacaggcgg ttgagcgctc
     7681 gaatccgtgg agtcacaaca cctttccggg cggacgtgcc gccagttgtg agcatgatga
     7741 gcgtgccctt tttccacgtc ggcggcgcca tcggcgtgct cggaaacctg tactcgggc
//
LOCUS       DASRJA010000452         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_335928,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000452 DASRJA010000000
VERSION     DASRJA010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335928"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VFC01_19925"
     CDS             <1..1086
                     /locus_tag="VFC01_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN81933.1"
                     /translation="YLSLGAALGLLASWLLSRRIKRHTRGLEIAEIAGLAEHREALLH
                     SIREGVVAVNNDGDITLLNDSAQELLGVSAAAVGRRVDGVGLEPAVVEFLLSGEDGRD
                     VVIATRTRVLALNRRAATSQGQRIGTVTTMRDSTELAALQGQLSSHKSVTDTLRAQTH
                     EFANQLHTISGLVQLGEYDSVRDLVGTLTRRRAEISDAVTQRISDPAVAALLIAKTTL
                     AAESGVSLEIAPQSRLAALDPALATDVITLLGNLVDNAVDVSVGSDPAKVAVAINDHD
                     GLMISVSDSGPGVPEHLREEIFARGVTSKPDVPGGRGIGLALVRLVTAQHGGSVEVSD
                     GPSGGALFAVLLPVGTVVAAAEGAAHA"
     gene            1079..1750
                     /locus_tag="VFC01_19930"
     CDS             1079..1750
                     /locus_tag="VFC01_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN81934.1"
                     /translation="MRDVLVVDDDFMVAEIHRRFVEQVDGFRAVGVARTGAEGLSAAR
                     ELQPQLILLDVYLPDMTGLEVLQRLRSEGDRVGVIMITAARELDTVKGALDGGAADYL
                     IKPFEFGQLKAKLEAFAARADALESAGGVDQSLIDSLFGGPAAASPQAVLPKGLGAET
                     GRLVLDAVRDAGEVSAAECADLVGISRVSARRYLEHFLGSGALELRLQYGVGRPERRY
                     RLGGV"
     gene            complement(1736..>2664)
                     /locus_tag="VFC01_19935"
     CDS             complement(1736..>2664)
                     /locus_tag="VFC01_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HZN81935.1"
                     /translation="CLDVEDRRRLDAELCADVSRLEGMGDARITAAAKETAARLDAQA
                     VVDRAAKAEADRTVTIRPAPDCMAYVSALLPVAQGVGVYAALKRAADTTFDDRSSGQV
                     MADTLVERVTGRPAEEPEPVSLNLVMSDQALWGQDNAPAVLEGYGPIPASLAQRLVKD
                     AVVDARSLATLRRLYRDPKSGSLVAMESRSRFFPKGLAEFIGLRDRTCRTPYCDAPIR
                     HRDHATPRNRGGPTSATNGLGECERCNYAKEAPGWRVAASDENGTHTAQFVTPTGARY
                     RSAAPPLPGAPLVAVSEVAVRIGVALADLHAA"
BASE COUNT          410 a          867 c          925 g          462 t
ORIGIN      
        1 tatctcagcc ttggtgcagc gctggggctg ctggcgtcgt ggctgctgtc gcgacggatc
       61 aaacggcaca cccgcgggct ggagatcgcc gagattgccg gcctggccga acaccgggaa
      121 gcattgctgc acagcatccg cgagggtgtg gtcgcggtga acaacgacgg cgacatcaca
      181 ctgctcaacg acagcgcgca ggaactactc ggggtgtccg cggccgccgt cggtcgccgc
      241 gtcgacggtg tcggcctgga accagcggtg gtcgaattct tgttgtcggg cgaagacggc
      301 cgcgatgtcg tgatcgccac gcggacacgc gttttagcgt tgaaccggcg ggcggccacc
      361 agtcaagggc agcgcatcgg aacggtgacg accatgcgcg acagtaccga actggctgcg
      421 ctgcaggggc agctgtcgtc gcacaagagc gtgaccgaca cgctgcgcgc gcagactcac
      481 gagttcgcga accagctaca caccatctcg gggctggtgc agctcggcga atacgactcg
      541 gtgcgtgacc tggtgggaac gctgacacgg cgccgcgcgg agatcagcga cgcagtgaca
      601 caacggattt ccgatcccgc cgtcgcggcg ttgttgatcg cgaagacgac gctggccgcg
      661 gagagtgggg tgtcgctgga gatcgcgccg cagtcgcgac tcgcggcgtt ggacccggcc
      721 ctcgccaccg acgtgatcac cttgctgggc aacctcgtcg acaatgcagt cgacgtgtcg
      781 gtgggttccg acccggcgaa ggtcgctgtc gccatcaacg accatgatgg actgatgatt
      841 tcggtgtccg actccgggcc tggtgtgccc gaacatcttc gggaagagat attcgcccgc
      901 ggggtcacct cgaaaccaga tgttcccggc ggccggggta tcggcctggc attggtccgg
      961 ctcgtcaccg cgcagcacgg cgggtcggtg gaggtcagcg acgggccttc tggtggggcg
     1021 ttattcgcgg tgcttctacc ggtcgggacg gtcgtggcgg cggcagaggg ggctgcgcat
     1081 gcgtgacgtg ctggtggtcg acgatgattt catggtcgcc gagatccacc gccgattcgt
     1141 cgagcaggtc gacggctttc gcgcggtggg cgtggcgcga accggcgccg aggggctgtc
     1201 ggcggctcgc gagctacagc cgcagctcat cctcctcgac gtgtatctcc cggacatgac
     1261 cggcctggag gtgttgcagc gactgcgatc tgagggcgat cgggtgggcg tcatcatgat
     1321 cacggcggcg cgcgaactcg acacggtcaa gggcgccctg gacggcggtg ccgccgacta
     1381 cttgatcaaa ccgttcgagt ttggccaatt gaaggcgaaa ctggaggcgt ttgccgcccg
     1441 tgccgatgca ctggagtctg caggcggtgt ggatcagtcg ttgatcgact cgctgttcgg
     1501 cgggcccgcc gccgcgtcgc cacaggcggt actgccgaag gggctcggcg ctgagactgg
     1561 tcggttggtg ctggatgcgg tccgggatgc cggcgaggtg tcggcggccg aatgcgctga
     1621 cttggtgggc atttcacggg tcagcgcccg ccgctacctc gagcacttcc tcggcagcgg
     1681 tgcgttggag ctgcggctgc aatacggcgt cggccggccc gagcgacgtt accgcttagg
     1741 cggcgtgtag gtcggccagc gcgacgccga ttctgactgc gacttcactc acggcgacca
     1801 gtggtgcgcc cggtaagggt ggcgcggccg aacggtaccg tgcacccgtt ggcgtgacga
     1861 actgcgctgt gtgcgtgccg ttctcgtcac tggctgccac gcgccagccc ggtgcttcct
     1921 tcgcgtagtt gcaccgctcg cattcgccca aaccattggt ggcgctggtc ggcccgccgc
     1981 gattgcgcgg tgtggcgtga tcgcgatgcc gaatcggcgc atcacagtac ggcgttcgac
     2041 acgttcggtc gcgcaagcca atgaattccg ccaacccctt ggggaagaag cgggatcgcg
     2101 actccatcgc caccagcgac ccgctcttcg ggtcgcgata cagccgccgc agcgtggcca
     2161 acgaccgcgc atcgaccacc gcgtccttga ccaggcgctg cgccaacgac gccggaatcg
     2221 ggccatagcc ctccagcact gccggcgcgt tgtcctgccc ccacagcgcc tgatccgaca
     2281 tcaccagatt caaagacacc ggttcgggct cctcggccgg tcgccccgtt acccgctcca
     2341 cgagcgtgtc ggccatcacc tgtccgctgg atcggtcgtc gaaagtggtg tccgcggcac
     2401 gctttaacgc cgcgtacacg ccgacccctt gagcgacagg caacagcgcg gacacatacg
     2461 ccatgcagtc gggcgccggc cggatggtca cggtgcggtc cgcttcggct ttcgcggccc
     2521 tatcaacgac cgcttgcgcg tcgaggcggg cggcggtctc cttggcggcc gcggtaatcc
     2581 tcgcgtcccc catgccctcg agcctgctga cgtcggcgca cagttccgcg tccagccgtc
     2641 gtcgatcctc gacgtccagg cacg
//
LOCUS       DASRJA010000453         5538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1161661,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000453 DASRJA010000000
VERSION     DASRJA010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161661"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFC01_19940"
     CDS             <1..625
                     /locus_tag="VFC01_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561927.1"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HZN81936.1"
                     /translation="ANQDLVSAPRLDNQATCYAGLEAFLAAAPGEHMPVLVLFDHEEV
                     GSQSDHGAQSELLHTVLERITLAAGGGREDFLRRLPGSMVASGDMAHATHPNYPERHE
                     PGHLIEVNAGPVLKVQPNLRYATDGRTAAVFALACAQAEVPLQRYEHRADLPCGSTIG
                     PMTSARTGIPTVDVGAAQLAMHSAREVMGAQDVAAYSAALQAFLSPA"
     gene            641..1009
                     /locus_tag="VFC01_19945"
     CDS             641..1009
                     /locus_tag="VFC01_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN81937.1"
                     /translation="MALDVVNITFDSTDPDKLAEWWARAVDGTVNALAPGFFVTVSRP
                     GGGPGLAFQKVGDPTPGKNRVHIDFTSADMDAEVKRLVDLGATETGRGEAGPDFQWVV
                     MADPDGNAFCVAGGEVENAT"
     gene            complement(1002..1232)
                     /locus_tag="VFC01_19950"
     CDS             complement(1002..1232)
                     /locus_tag="VFC01_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005058245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81938.1"
                     /translation="MSTTSETGHVTGTKDKNYNLLWYTEQCLENALRLETYIQDAERD
                     GDNEVVELFRKAQSDSKKGAEEGKKLLASRIK"
     gene            complement(1275..1478)
                     /locus_tag="VFC01_19955"
     CDS             complement(1275..1478)
                     /locus_tag="VFC01_19955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81939.1"
                     /translation="MARAGRRSPSTTPDAVRPAWDEALAAQAPVVLDMRTDPEVPPIS
                     PQIAKNKAAELLPRAGLHSKIDG"
     gene            1581..3878
                     /gene="purL"
                     /locus_tag="VFC01_19960"
     CDS             1581..3878
                     /gene="purL"
                     /locus_tag="VFC01_19960"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HZN81940.1"
                     /translation="MTSELTHALDTVDRAAATPEQPQPFRELGLKDDEYQRIRQILGR
                     RPTGAELAMYSVMWSEHCSYKSSKVHLRYFGETTTEAMRASMLAGIGENAGVVDIGDG
                     WAATFKVESHNHPSYIEPYQGAATGVGGIVRDIMAMGARPVAVMDQLRFGAADAPDTR
                     RVVDGVVRGIGGYGNSLGLPNIGGETVFDASYAGNPLVNALCVGVLRKEDLHLAFASG
                     TGNKIILFGARTGLDGIGGVSVLASETFGGDESGAPGRKKLPSVQVGDPFMEKVLIEC
                     CLELYANDLVIGIQDLGGAGLSCATSELASAGDGGMRIELDAVPLRAANMTPAEILSS
                     ESQERMCAVVAPENVEKFMAVCGKWEVLATVIGEVTDGDRLQITWHGETVVDVPPRTV
                     AHEGPVYERPVQRPDTQDALNADTSAKLPRPSTGEELRATLLALLGSPHLCSRAFITE
                     QYDRYVRGNTVLAEHADGGVLRVDEATGRGITVSTDASGRYTQLDPYAGAQLALAEAY
                     RNVAVTGAIPVAVTNCLNFGSPEDPGVMWQFSQAVRGLADGCAALGIPVTGGNVSFYN
                     QTGTTPILPTPVVGVLGVIDDVKRRIPTGLGTEPGETLILLGDTRDEFDGSIWAQVTA
                     DHLGGLPPRVDLQREKLLAEVLAAASRDGLVSAAHDLSEGGLMQAVVEAALAGETGCR
                     IIIPEGSDAFVTLFSESAGRALVAVPRTEESRFRAMCEARGLPATRIGVVDQGSDAVE
                     VQGLFTVSLEDLRSTSEGVLPGLFG"
     gene            3875..>5538
                     /locus_tag="VFC01_19965"
     CDS             3875..>5538
                     /locus_tag="VFC01_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN81941.1"
                     /translation="MTVAPRERHPLLVWAWSLVHLDFVGVAFGALFFCLSLTPSLLPR
                     DWLFEGLIGGLNAAIGYGIGAFAGKMVRRFVLRRRKWWPPSKPVLYALKTITVAGSVT
                     ACVLMLIPAAAWQRQVSAVMGMEGPNTLGYTRSLIIAVLVGGACVGVARVLLDVIKTM
                     ARFFIRRWHLHDEMALFIGTAIVVVLAVTLINGVLVRGFLAGANRVFQPQNTTTRAGI
                     VQPQQPERSGSPTSFAPWETLGFQGRNFVATGPHADELTRLNGGPAKEPIRVYVGLNT
                     ADDDESRMAVLLSELERTGAFDRKVLVIVPTTGTGWINPVAARAVEMMYNGDTAMVAS
                     QYSFLPSWISFLGDKEKSMASGRMMIDAIHERWMQLPADHRPKLVLYGESLGSMAGQG
                     AFGWLPDISRMGFSSVLWVGPPNASPIWSGVIARRDPGTTEVEPRYDNGRTVRFSQGV
                     DANEIHNDTTAPWEGTRVLFLQHPSDPIVWWSEDLLFNRPDWLREPPGRDRTASMRWY
                     PIITFWQVAADMTNASSVPGGHGHNYGDFVLDGWAAVAPPDGWTPD"
BASE COUNT          858 a         1804 c         1915 g          961 t
ORIGIN      
        1 agccaaccag gatctggtga gcgcgccccg gctggacaac caggcgacct gctacgcggg
       61 gctggaagcg ttcttggccg ccgcgcccgg cgagcacatg cccgtgctgg tgctgttcga
      121 ccacgaggag gtcggctcgc agtcggatca cggcgcccag tcagagctgc tgcacacggt
      181 gctggagcgg atcacgctgg cggcgggcgg cggtcgggag gacttcctgc gccgactgcc
      241 gggctcgatg gtggcgtccg gcgacatggc gcacgccacc cacccgaatt accccgagcg
      301 acacgagccc ggccacctca tcgaggtgaa cgccgggccg gtgctcaaag tgcaaccgaa
      361 tctgcgctat gccaccgacg gccgcaccgc ggccgtgttc gcgctggcgt gcgcgcaggc
      421 cgaagtgccg ctgcagcgct acgagcatcg ggctgacctg ccgtgtgggt cgaccatcgg
      481 gcccatgacc tccgcgcgca ccggcatccc gaccgtcgac gtgggcgcgg cccagctggc
      541 catgcattcg gcgcgggaag tgatgggtgc gcaggacgtg gccgcctact cggcggcact
      601 tcaggcgttt ttgtcaccgg cctgatctag ggtcagcgcc atggcacttg acgtcgtgaa
      661 catcacgttc gattccacag atcccgacaa gcttgccgag tggtgggccc gcgccgttga
      721 cggcacggtg aacgctttgg cgccgggctt tttcgtcacg gtgagccggc ccggcggcgg
      781 ccctgggctg gcgttccaga aggttggcga tccgacgccc ggtaagaacc gcgtgcacat
      841 cgacttcacc tccgcggata tggacgcgga ggtgaagcga ctggttgacc ttggcgccac
      901 tgaaaccggc cgcggcgaag ctggcccgga cttccagtgg gtggtgatgg ccgaccccga
      961 tggcaacgcg ttctgcgtcg ccggtggtga ggtggagaac gctacttgat ccgggaggcg
     1021 agtagcttct tgccctcttc ggcacccttc ttgctgtccg actgcgcctt gcggaacagc
     1081 tcgacaactt cgttgtcgcc gtcgcgctcg gcgtcctgga tgtaggtctc gagccgcaac
     1141 gcgttctcga ggcactgctc ggtgtaccac agcaggttgt agttcttgtc cttggttccc
     1201 gtcacatgac cggtttcgct ggtcgtgctc atgcgtcacc ttccttcgct tgtcattgcc
     1261 gtgacaaaga tcccctaccc gtcgatcttg gagtgcaaac cggcacgcgg aagtagttcg
     1321 gccgctttgt tcttcgcgat ttggggtgaa atcggcggca cttcgggatc ggtgcgcatg
     1381 tccagaacga cgggcgcctg ggcggccagc gcctcatccc atgccggtcg gaccgcgtcc
     1441 ggggtcgtcg acggcgatcg ccttccggcc cgtgccaccc attgcgaggt gcgcgtactt
     1501 ggccccgatc gcataggcac cggtttgggc ccaagtgcgt cgcgataccc ccgcgcatcg
     1561 ggccaaacta gactggccgc gtgacgtcgg agctcaccca cgcccttgac accgttgacc
     1621 gcgccgccgc cactcccgag caaccgcagc catttcgcga actcggcctg aaggacgacg
     1681 agtaccagcg gatacgccag atcctcggcc gcaggcccac cggcgccgag ctggccatgt
     1741 actcggtgat gtggagcgaa cactgctcct acaagtcctc caaggtgcat ctgcgctact
     1801 tcggcgagac caccaccgag gcgatgcggg cctcgatgct ggcgggcatc ggcgagaacg
     1861 ccggcgtcgt cgacatcggc gacggctggg ccgcgacctt caaggtggag tcccacaatc
     1921 acccgtcgta catcgagccg tatcagggcg cagccaccgg tgttggtggc atcgtgcgcg
     1981 acatcatggc gatgggcgcc cggccggttg ccgtcatgga tcagctgcgg ttcggcgccg
     2041 ccgacgcgcc cgacacgcga cgggtggtcg acggcgtggt gcgtggcatc ggcggctacg
     2101 gaaactcgct gggactgccc aacatcggcg gcgagacggt gttcgacgcc tcgtacgccg
     2161 gcaacccgtt ggtcaacgcg ttgtgcgtcg gggtgctccg caaagaggat ctgcacctgg
     2221 cgttcgcgtc gggcacgggc aacaagatca tcctgttcgg cgcgcgcaca ggtctggacg
     2281 gcatcggcgg cgtgtcggtg ctcgcctcgg agaccttcgg tggcgacgaa tcgggcgcgc
     2341 ccggacgcaa gaagctgcca agcgttcagg tcggcgaccc gttcatggag aaggtgctca
     2401 tcgagtgctg tctcgagctg tacgccaacg acctggtgat cggcatccag gacctgggcg
     2461 gtgccgggtt gtcctgtgcc acatccgaac tcgcgtcggc aggcgatggc ggcatgcgga
     2521 tcgagctgga cgcggtgccg ctgcgggccg cgaacatgac gcccgcggag attctgtcca
     2581 gcgagtcgca ggagcggatg tgcgcggtcg tcgcgccgga gaacgtcgag aagttcatgg
     2641 cggtgtgcgg caagtgggag gtgctggcca ccgtcatcgg tgaggtcacc gacggcgacc
     2701 ggctgcagat cacctggcac ggcgagacgg tcgtcgacgt gccaccgcgc acggtcgcgc
     2761 acgagggtcc ggtctacgag cgtccggtgc aacgaccgga cactcaggac gcgttgaatg
     2821 ccgacacgtc ggccaagctg ccgcggccat caacaggcga ggagctgcgg gcgactttgc
     2881 ttgcgctact tggcagtccg cacctgtgca gccgcgcatt catcaccgag cagtatgacc
     2941 gctacgtgcg aggcaacacc gtgctcgccg aacacgccga cggtggcgtg ctgcgcgtcg
     3001 acgaagccac gggccgtggc atcacggtgt ccaccgacgc gtccggccgc tatacccagc
     3061 tcgacccgta cgccggtgcg cagctagcgc tggccgaggc gtaccgcaac gttgcggtca
     3121 ccggcgccat accggtcgca gtgaccaact gcctcaattt cggctcgccg gaggatcccg
     3181 gtgtcatgtg gcagttcagc caggccgtcc gcgggctcgc cgacggctgt gcggcccttg
     3241 gcattccggt caccggcggc aatgtcagct tctacaacca gaccggaacc acgccgattc
     3301 tgcccacccc ggtcgtcggc gtgctcggtg tcatcgacga tgtcaagcgg cgcattccga
     3361 ccggcctggg caccgagccg ggggagacgc tgatcttgct cggtgacacc cgcgacgagt
     3421 tcgacggttc gatctgggcg caggtcaccg ccgaccatct cggcgggctg ccgccgcgcg
     3481 tggacctaca gcgcgagaag ctgctcgccg aggtgctcgc cgcggcatcg cgcgacggcc
     3541 tggtgtcggc cgcgcatgat ctgtccgagg gcgggctgat gcaagccgtc gtcgaggcgg
     3601 cacttgcagg cgaaaccggt tgccgcatca tcattcccga gggctcggac gcgtttgtga
     3661 cgctgttctc cgagtccgcg ggccgggcgc tggtcgcggt gccgcgcact gaggagagca
     3721 gattccgcgc gatgtgtgag gcgcgcgggc tgcctgcgac acgcatcggt gtcgtcgacc
     3781 aaggcagcga cgcggtcgaa gtacaaggct tgttcactgt ttccctggag gatctgcgca
     3841 gcacgtcgga gggcgtgctt cccgggttgt tcgggtgact gtcgcgcctc gcgaacgaca
     3901 tccgctactg gtctgggcgt ggagcctggt acacctcgac ttcgtcgggg tggcgttcgg
     3961 cgctctgttc ttctgcctgt cgctgacccc gtcgctgctg ccgcgcgact ggctgttcga
     4021 ggggctgatc ggtggcctca acgccgccat cggttacggc atcggtgcct tcgccggaaa
     4081 aatggtgcgc cgcttcgtgt tacgtcgccg caagtggtgg ccgccgtcga agccggtgct
     4141 ctatgcgctg aagaccatca ccgtcgcggg atcggttacg gcgtgcgtgc tgatgctgat
     4201 tcccgcggcg gcgtggcagc gtcaagtgtc ggcggtgatg ggcatggagg gtcccaacac
     4261 gctgggctac acgcgttcgc tgatcatcgc cgtgctggtg ggtggcgcgt gcgtcggcgt
     4321 cgcgagggtg ctgctcgacg tgatcaagac gatggcgcgg ttcttcatca ggcgctggca
     4381 tctgcacgac gagatggcgt tgttcatcgg caccgcgatc gtggtggtgc tggccgtcac
     4441 gctgatcaac ggtgtgctcg ttcgtggctt cctggccggc gcgaaccgcg tgttccaacc
     4501 gcagaacacc accacccggg cgggtatcgt ccagccgcaa cagcccgagc ggtccggcag
     4561 cccaacgtct ttcgcgccgt gggagacact tggattccag ggtcgcaact tcgtcgccac
     4621 cggcccgcac gccgacgaac tcacacgcct gaacggcgga ccggccaagg agccgatccg
     4681 ggtatatgtc ggcctgaaca ccgcggacga cgacgagagt cgaatggcgg tgctgctgag
     4741 cgaactcgaa cgcaccggag cgttcgaccg caaggtgctc gtcatcgtcc cgaccacggg
     4801 caccggatgg atcaacccgg tggccgcgcg ggccgttgag atgatgtaca acggcgacac
     4861 cgcgatggtc gcatcgcagt actcctttct gccgagctgg atttcgttcc tcggcgacaa
     4921 ggagaagtcg atggcgtcgg gccggatgat gatcgacgcc atccacgaga ggtggatgca
     4981 actgccggcc gaccaccggc ccaagctcgt gctgtatggc gagagtctcg gctcgatggc
     5041 cgggcagggc gcgttcggct ggctgccgga catctcgcgc atgggctttt cgtcggtatt
     5101 gtgggtcggc ccgcctaacg cgagcccgat ctggagcggc gtcatcgcgc ggcgcgaccc
     5161 gggcaccacg gaagtcgaac cgcgctatga caacgggcgt accgtgcggt tctcgcaggg
     5221 cgtcgacgcc aacgagatcc acaacgacac gacggcgccg tgggaaggta cccgggtgct
     5281 gttcttgcag catccgtcgg acccgatcgt ctggtggtcg gaggacctgc tgttcaaccg
     5341 gccggactgg ctgagggaac cgccgggacg cgaccgcacg gcgtcgatgc gctggtatcc
     5401 gatcatcact ttctggcagg tggccgccga tatgaccaat gcgtcgtcgg ttcccggcgg
     5461 gcatggccac aactacgggg acttcgtgct cgacgggtgg gccgctgtcg cgccgcccga
     5521 cggctggacg cccgacga
//
LOCUS       DASRJA010000454         4312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_847041,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000454 DASRJA010000000
VERSION     DASRJA010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847041"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1400
                     /locus_tag="VFC01_19970"
     CDS             156..1400
                     /locus_tag="VFC01_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HZN81942.1"
                     /translation="MESRVSPQAPGAHLPDHPDFLWRNPEPKRSYDVVIIGGGGHGLA
                     TAHYLVKNHGITNVAVLEKGWLAGGNMARNTTLIRSNYLWDESAQIYEHSLKLWEGLE
                     DDLGYPILFSQRGVLNLAHSLQDVRDSVRRVEANVLNGVDAEWLEPDEVKKLCPIVNI
                     SADLRYPVLGATYQPRAGIAKHDYVAWGFARRADAAGVDLLQGCEVTGFITDGDRVTG
                     VRTTLGDIAAGQVAMCAAGHTSVLTDMLGFRVPLQSHPLQALVSELLEPVHPTVVMSN
                     AVHVYVSQAHKGELVMGAGIDSYNGYGQRGAFHIIERQMAAAVELFPIFARAHLLRTW
                     GGIVDVSPDASPIIGRTPYSNLYLNCGWGTGGFKATPGIGWCLADTIARDEPHPYVAP
                     FSLDRFVTGALVDEHGAAAVAH"
     gene            1424..1702
                     /locus_tag="VFC01_19975"
     CDS             1424..1702
                     /locus_tag="VFC01_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005252386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HZN81943.1"
                     /translation="MQLIECPWCGPREETEFHYGGQAHVAYPDDPFALSDEQWAHYVF
                     FRANTKGQFAERWVHSAGCRRWFNAVRDTATYRFHSVYRIDEPKPVIS"
     gene            1699..>4312
                     /locus_tag="VFC01_19980"
     CDS             1699..>4312
                     /locus_tag="VFC01_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZN81944.1"
                     /translation="MTAPFRTATGGRIDRGTRYEFTFDGRTYSGHRGDTLGSALLAHG
                     VHKIATSVKLGRPRGISAAWAEDTGGLVQIEAPFPEPMLLASTVELHDGLAARGIPGQ
                     GRLADIADSARYDAMHAHADVLVVGAGPAGLMAALTAARAGARVVLVDEQSEAGGSLL
                     GGTDDIEWVRAAVAELATYSEVRHLQRTTAFGHYDDGFVLALERRTDHLGNAAPTRLS
                     RQRVWRIRARHVLIAAGAHERPVVFADNDRPGIMLAHSARTFLHRYGVLVGRSAVLFT
                     TNDSAYAAAFDLADAGVRIEAIVDARELVSDALRDECERRGILLRAAAVVTGTRGDDR
                     ITDALVADFDGVNVGAAEAVSCDALLVSGGWNPAVHLFSQARGVLRYDPALGGFVPGE
                     PLAGVSVAGAANGVMTLAGCLREGRDSAKAALSELGLPFGNSSPRPDVAVDETVPGLV
                     LWRVPGPQQDQFVDVQRDATVADVARAVGAGMRSVEHIKRYTTIGTAHDQGKTSGVIA
                     SGITAELLGLPIEALGTTTFRPPYTPVAFAALAGRDRGTLFDPVRTTALHDWHVAQGA
                     VFEDVGQWKRPRHYPRPGEDMDAAVLRECAAARTGVGILDGSTLGKIDVSGRDAAELL
                     DRIYTNLMSSLKVGSIRYGVMCGVDGMVIDDGTVLRLAEDRFLVYTTTGGAAKILDWM
                     EEWLQTEWPQLQVALTSVTDHWATFPVVGPRSRDVIGAVFGDVDVSNEAFPFMTWRDT
                     KLDGVSVRVARVSFSGELAFEVNVNWWHAPAVWQRLLEAGRPYGITPYGTETMHVLRA
                     EKGYPIIGQDTDGTVSPHDLGMAWAVSKKKPDFVGKRSFSRAENHNPLRKQLVGLLPV
                     DGITRLPEGSQIVEF"
BASE COUNT          702 a         1434 c         1471 g          705 t
ORIGIN      
        1 gcaaggtcgg cgttggactg ggtggcaccc tcgccggtct cggccagctc cacgctcatc
       61 gctcgtctcc cgtcggacat tgactcttga ccgcgcgtat caacgtacca tactgtggcc
      121 cacaactgat atatcagtta gacaacgact gttcgatgga gagccgtgtg agtccacagg
      181 cgcccggcgc gcacctgccc gatcatcccg acttcctctg gcgcaatccg gagcccaagc
      241 ggtcatacga cgtcgtgatc atcggcggcg gaggccacgg cctggcgacc gctcattacc
      301 tcgtgaagaa ccacggcatc acgaacgttg cggtgctcga gaagggctgg cttgccggcg
      361 gcaacatggc ccgcaacacc acgctgatcc ggtcgaacta tctgtgggac gagagcgcgc
      421 agatttacga gcactcgctc aagctgtggg agggccttga ggacgacctc ggctacccga
      481 tcctgttcag ccagcgcggc gtgctcaacc tcgcgcacag tctccaggat gtccgtgaca
      541 gcgtgcgccg cgtagaagcc aacgtgctca acggcgttga cgccgaatgg ctggaacccg
      601 acgaggtcaa gaagctgtgc ccgatcgtca acatctcggc cgatctgcgc tacccggtac
      661 tcggcgcgac ctaccaaccg cgcgcgggaa tcgccaaaca cgattacgtc gcttggggtt
      721 tcgcgcggcg agccgacgcg gccggcgtcg atctgctgca gggctgtgag gtcaccggct
      781 tcatcaccga cggcgaccgg gtgaccggcg tgcgcactac cctcggcgat atcgcggccg
      841 gacaggtcgc gatgtgcgcg gccgggcaca cgtcggtgct taccgacatg ctgggcttcc
      901 gggtgccgtt gcagagccat ccgttgcagg cgttggtatc cgaactcctc gagcccgtgc
      961 acccgacggt ggtgatgtcc aacgccgtgc acgtctacgt ctcgcaggcg cacaagggcg
     1021 agctcgtgat gggcgcgggc atcgactcgt acaacggcta cgggcagcgc ggcgccttcc
     1081 acatcatcga gaggcagatg gccgcggctg tcgagctgtt cccgattttc gctcgcgcgc
     1141 atctgctgcg gacctggggc gggatcgtcg atgtcagccc cgacgcctcg ccgatcatcg
     1201 gccgcacgcc gtacagcaac ttgtatctca actgtggctg gggcacaggc ggattcaagg
     1261 ccactccagg catcggctgg tgtctggccg acaccatcgc ccgcgacgag ccacacccct
     1321 acgtggcgcc gttcagcctg gaccgcttcg tcaccggagc gctggtcgac gaacacggcg
     1381 ccgcagcggt cgcgcactga aacttcgaga ggctggagca acgatgcaac tcatcgaatg
     1441 cccgtggtgc ggaccgcggg aagagacgga attccactac ggcgggcagg cacacgttgc
     1501 ctatcccgac gacccgttcg cgctgtccga cgaacagtgg gcgcactacg tgttcttccg
     1561 ggccaacacc aaaggccagt tcgccgaacg gtgggttcac agcgcgggat gccgtcgctg
     1621 gttcaacgcc gtccgtgaca ccgccaccta tcgcttccac agcgtgtacc gcatcgacga
     1681 gccaaagccg gtgatctcgt gaccgcgccg ttccgcaccg caaccggagg ccgcatcgac
     1741 cgcggcaccc gttacgaatt cacgttcgac ggtcgcacgt acagcggcca ccgcggcgac
     1801 accctgggct cggcgctgct ggcgcacggg gtgcacaaga tcgcgacgag cgtcaaactc
     1861 ggccgcccgc gtggcatttc ggcggcatgg gccgaagaca ccggcgggct ggtgcagatc
     1921 gaggcgccct tcccggagcc gatgctgctg gcgtcgacgg ttgaactgca cgacgggctc
     1981 gccgcccgcg gaatccccgg gcagggccgc ctggccgata tcgccgactc cgcacggtac
     2041 gacgccatgc atgcgcacgc ggacgtgctc gtcgtcggcg ccgggccagc gggactgatg
     2101 gcggcactga ccgccgcgcg tgccggcgcc agggtggtgc tggtcgacga gcagtccgaa
     2161 gccggcggct cgctgctcgg cggcaccgac gacatcgaat gggttcgggc ggcggtcgcc
     2221 gaactggcga cgtactccga ggttcgacac ctgcagcgca ccaccgcgtt cggtcactac
     2281 gacgacggct tcgtgctggc cctcgagcgg cgcaccgacc atctcggaaa cgcggccccg
     2341 acccggctgt cgcgtcagcg ggtgtggcgg atccgcgcgc ggcacgtgct gatcgcggcc
     2401 ggtgcgcatg agcgccctgt cgtattcgct gacaacgacc ggcccggaat catgttggcg
     2461 cacagcgcca gaacgttcct gcatcgatac ggcgtgttgg tcggacgctc cgcggtgttg
     2521 ttcaccacga acgacagcgc ctacgcggcg gcgttcgacc tcgccgatgc cggcgtgcgc
     2581 attgaggcga ttgtcgatgc ccgcgaactc gtgtctgacg ccctgaggga tgagtgcgag
     2641 cgacggggca tcctgttgcg tgcggccgcg gtggtgaccg gcactcgggg cgacgaccgc
     2701 atcaccgacg ccttggtggc ggactttgat ggtgtgaatg tcggtgccgc tgaggctgtt
     2761 tcgtgcgacg cgctgctggt gagtggtggc tggaatccgg cggtgcacct gttcagccag
     2821 gcccgtggcg tgctgcgcta cgacccggcg cttgggggct tcgtccccgg cgagcccctc
     2881 gccggcgtga gcgtcgcggg cgctgccaat ggtgtcatga ccctggccgg ctgcctgcgc
     2941 gaaggccgcg actccgccaa agcggcgctg tccgaactgg gcctaccatt cggtaattcc
     3001 tcgccgcgtc ccgacgtcgc ggtcgacgag accgtgccag ggctggtgct gtggcgagtg
     3061 cccggcccgc agcaggatca gttcgtcgat gtgcagcgcg acgcgacggt cgcagacgtg
     3121 gcgcgcgcgg tcggcgcggg catgcgatcg gtcgaacaca tcaagcgcta caccacgatc
     3181 ggcaccgccc acgatcaagg caagacatca ggggtcatcg cgtcggggat caccgccgaa
     3241 ctgctcggcc tgcccatcga agcgttgggc accacgacat tccggccgcc ctacacgccc
     3301 gttgcgttcg cggcccttgc ggggcgcgac cgtggcacgc tgttcgaccc cgtgcgcacc
     3361 accgcgctgc acgactggca tgtcgcacag ggcgcggtgt tcgaggacgt tgggcaatgg
     3421 aagcgtccgc gtcattaccc acgccccggc gaggacatgg acgctgcggt gctgcgcgaa
     3481 tgcgccgcgg cgcgtaccgg tgtcggcatt ctcgacggct ccacgctcgg caagatcgac
     3541 gtgtccggcc gcgacgccgc cgagctactg gaccggatct acaccaacct gatgagcagc
     3601 ctgaaggtcg gctcgatccg gtacggcgtg atgtgtggcg tcgacggcat ggtgatcgac
     3661 gacggcaccg tgttgcggct ggccgaggac cggttcctgg tgtacacgac caccggcggg
     3721 gcggcgaaaa tccttgactg gatggaggag tggctgcaga ccgagtggcc gcagttgcag
     3781 gtcgcgctca cgtcggtgac cgatcactgg gcgacgttcc ccgtggtggg gccgcggtcc
     3841 cgcgacgtaa tcggggccgt gttcggcgac gtggacgtgt ccaacgaagc cttcccgttc
     3901 atgacgtggc gcgacaccaa actcgacggg gtgtccgtca gggtggcgcg ggtgagcttc
     3961 tccggtgaac tcgcgttcga ggtgaacgtg aactggtggc atgcgcccgc ggtgtggcag
     4021 cgcctgctgg aggcgggccg cccctacggc atcacgccgt acggcaccga gaccatgcat
     4081 gtgctgcgcg ccgagaaggg ctacccgatc atcgggcagg acaccgacgg tacggtcagc
     4141 ccgcacgatc tcggaatggc gtgggcggtg tcgaagaaga agcccgactt cgtcggcaag
     4201 cggtcgttca gccgggccga aaaccacaat ccgttgcgca agcagctggt cgggttgtta
     4261 ccggtcgacg ggatcacccg actcccggag ggttcgcaaa tcgtggagtt ct
//
LOCUS       DASRJA010000455         4372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_301397,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000455 DASRJA010000000
VERSION     DASRJA010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301397"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1932)
                     /locus_tag="VFC01_19985"
     CDS             complement(199..1932)
                     /locus_tag="VFC01_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZN81945.1"
                     /translation="MKPDYDVLIIGSGFGGSVTALRLTEKGYRVGVLEAGRRFNDEDF
                     AKTSWNLRKFLWAPKLGMYGIQRIHLLRNVMILAGAGVGGGSLNYANTLYVPPDPFFN
                     DPQWKNITDWHSELMPHYDQAQRMLGVVQNPTFTDADRVMKEVADEMGIGDTFVPTPV
                     GVFFGPDGNKAPGKTVPDPYFGGAGPARTGCIECGSCMTGCRYGAKNTLLKNYLGLAE
                     RAGAEVIPMTTVTGLEQRPDGSWKVRTARTGLLPFKRRTYTASHVVLAAGTWGTQKLL
                     FKMRDKGKLPKLSDKLGVLTRTNSESIVGAGRFNVSPDLNLTHGVAITSSIHPTSDTH
                     VEPVRYGKGSNAMGLLQTLMTDGAGPQGTDVPRWKQFLQQAGEDPRAMLRLLNPRRWS
                     ERTMIALVMQHLDNSITTFTRRTKLGFRRLDSKQGHGEPNPSWIPAGNEVTRRIAKKI
                     DGVAGGTWGELFNIPLTAHFLGGAAIGDTAEHGVIDPYQRVYGYPTLSVHDGASVSAN
                     LGVNPSLSITAQAERAASLWPNKGQVDQRPAQGQPYKRLAPIPPEHPVVPADAPAALR
                     QLPIEPVSSAG"
     gene            complement(2016..3143)
                     /locus_tag="VFC01_19990"
     CDS             complement(2016..3143)
                     /locus_tag="VFC01_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HZN81946.1"
                     /translation="MVEIGMGRTARRTYELDDINIVPSRRTRSSQDVSTAWQMDAYRF
                     EIPVVSHPTDALVSPEFAIELGRLGGLGVLNGEGLIGRHADVEEKISQVIEAAEKEPE
                     PSAAIRLLQQLHSAPLDPDLLGAAVARIREAGVTTAVRVSPQNAQSLTPSLVAAGIDL
                     LVIQGTIISAERVAQDGEPLNLKTFISELDVPVVAGGVLDHRTALHLMRTGAAGVIVG
                     YGSTSGVTTSDEVLGISVPMATAIADAAAARREYLDETGGRYVHVLADGDIHTSGDLA
                     KAIACGADAVVLGTPLSTAAEALGGGWFWPAAAAHPSLPRGALLQVAIGERPPLAQVL
                     TGPSDDPFGSLNLVGGLRRSMAKAGYCDLKEFQKVGLTVGS"
     gene            complement(3162..>4372)
                     /locus_tag="VFC01_19995"
     CDS             complement(3162..>4372)
                     /locus_tag="VFC01_19995"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZN81947.1"
                     /translation="GMVTDPVTCSPDNTLAEVDAMCARFRISGLPVVDADGALVGIIT
                     NRDMRFEVDMSKPVSEVMTKAPLITAQAGVSAEAALGLLRRYKIEKLPIVDGHGKLTG
                     LITVKDFVKTEQFPLSTKDSDGRLLVGAAVGVGDDSWTRAMTLVDAGVDVLVVDTAHA
                     HNRLVLDMVHRLKTLVGERVDVIGGNVATRAAASALVQAGADAVKVGVGPGSICTTRV
                     VAGVGAPQITAILEAVAACAPKGVPVIADGGLQYSGDIAKALAAGASTTMLGSLLAGT
                     AESPGELIFVNGKQFKSYRGMGSLGAMQGRGGAKSYSKDRYFQDDALSEDKLVPEGIE
                     GRVPFRGPLSTVIHQLTGGLRAAMGYTGSATIEQLQQAQFVQITAAGLKESHPHDITM
                     TVEAPNYTSH"
BASE COUNT          700 a         1497 c         1423 g          752 t
ORIGIN      
        1 tgcaccgccg cccccgctgc cgctcgccgc cgcgctcgtc gtggttgtcg tcgtcgtggt
       61 ggtcgtgctt gttgtcgccg gtgcggtggt tggtcaacga caacggaatc tcgcttcctc
      121 gcaaagttac gacgggacag cgagattcat acctgcgcgc aggcgccggg caacaggacc
      181 gctacgtaga gcttcctgtc aacccgctga actgaccggc tcgataggaa gttgtcgcag
      241 cgcagcaggc gcatcggcgg gcaccaccgg atgctccggc ggaatcgggg cgagccgctt
      301 gtacggctgg ccctgtgctg gccgctgatc gacctggccc ttgttcggcc acagcgacgc
      361 tgcccgctcg gcctgcgcgg tgatcgaaag cgacggattc acaccgagat tcgccgatac
      421 cgaggcgccg tcgtgcaccg agagtgtcgg atagccgtac acccgctgat aggggtcgat
      481 caccccgtgc tccgcggtgt cgccgatcgc cgccccaccc aggaagtgcg cggtcagcgg
      541 gatgttgaac agctcgcccc aggtgccgcc cgccacgccg tcgatcttct tggcgatgcg
      601 tcgggtgacc tcgttgcctg ccgggatcca cgacgggttc ggctcgccgt ggccctgctt
      661 gctgtcgagt cgccggaagc cgagtttggt gcgccgcgtg aaggtggtga tcgagttgtc
      721 cagatgctgc atcaccaatg cgatcatcgt gcgctcgctc caccgccggg ggttgagcag
      781 ccggagcatg gcccgaggat cttcgcctgc ctgctgcagg aactgcttcc agcgcggcac
      841 gtcggtgccc tgcggccctg cgccgtcggt catcagggtc tgcaacagcc ccatcgcgtt
      901 tgaccccttg ccgtaccgaa cgggttcgac gtgcgtgtcc gatgtcggat ggatcgacga
      961 ggtgatcgcg acgccgtgcg tgagattgag gtcgggcgac acgttgaagc gtcccgcacc
     1021 cacgatcgac tcagagttgg ttcgggtcag cacgccgagc ttgtcggaca gcttgggcag
     1081 cttgcctttg tcgcgcatct tgaacaacag cttctgggtg ccccacgtgc ccgcggccag
     1141 cacgacgtgc gaggcggtgt aggtgcgccg cttgaacggc aacaatccgg tccttgcggt
     1201 gcgcaccttc cacgacccgt cgggccgctg ctccaggccg gtcaccgtcg tcatcggaat
     1261 gacttctgcc ccagcccttt ccgccaaccc gaggtagttc ttcaacaacg tgttcttggc
     1321 gccgtagcgg cagccggtca tgcaggagcc gcattcgatg cagcccgtcc gcgcgggccc
     1381 ggcaccgccg aaatagggat cgggcacggt cttgcccggt gccttattac cgtcgggccc
     1441 gaagaacacc ccgaccggag tcggcacgaa tgtgtcgccg atgcccatct catcggcgac
     1501 ctccttcatg acacggtcgg cgtcggtgaa ggtcgggttc tggaccacgc cgagcatgcg
     1561 ctgggcctgg tcgtagtgcg gcatcaactc ggagtgccag tcggtgatgt tcttccactg
     1621 cgggtcgttg aagaacgggt ccggcggcac atacaaggtg ttggcgtagt tcagcgaccc
     1681 gccgccgacc cccgcgcccg ccaggatcat gacgttgcgc agcaggtgga ttcgctggat
     1741 gccgtacatg ccgagcttcg gcgcccacaa gaacttgcgc aggttccagg acgtcttggc
     1801 gaagtcttcg tcgttgaacc ggcggcccgc ctcgagcacc ccgacgcgat agcccttctc
     1861 cgtgagccgc agcgcggtga cgcttccccc gaaccccgaa ccgatgatca gaacgtcata
     1921 gtcaggtttc acgcgaccag tatggactta acgatcgcta agttctagac aggggggcct
     1981 aatttgtaaa gcaggccctc gctgaccagg cagaattagc tgccgaccgt cagaccgacc
     2041 ttctggaact ccttcaggtc gcaatagccg gccttggcca tcgaccggcg cagcccgccg
     2101 accaggttca gcgagccgaa cgggtcatcc gacgggccgg tcagcacctg cgccagcggc
     2161 ggccgctccc cgatcgcgac ctgcagcaac gcgccgcgcg gcaacgatgg atgggccgca
     2221 gcggccggcc agaaccagcc gccgccgagc gcctcggccg ccgtcgaaag cggggtgccc
     2281 agcaccaccg cgtcggcgcc acacgcgatg gccttggcca ggtcgccgga cgtgtggatg
     2341 tcgccgtcgg ccagcacatg gacgtaccgg ccgccggtct cgtcgaggta ttcgcgacgg
     2401 gccgcggccg catccgcgat cgccgtcgcc atcggcacgc tgatgcccag cacctcgtcg
     2461 ctcgtcgtca cgccggacgt cgacccgtag ccgacgatca cacccgccgc cccggtgcgc
     2521 atcaagtgca gggcggtgcg atggtccagc acaccgccgg ccaccactgg gacgtcgagc
     2581 tcggaaataa aggtcttcag attcagcggc tcgccgtcct gcgcgacgcg ctcggccgaa
     2641 atgatggtgc cctgaataac gagcaggtcg atgcccgcgg cgaccagcga cggcgtcagt
     2701 gactgtgcgt tctgcgggct gacgcgcacg gccgtcgtca ccccggcctc gcggatccgc
     2761 gctaccgcag cgcccagcag atcgggatcc agtggcgccg aatgcaattg ctgcagaagg
     2821 cggatggccg ccgacggttc tggctccttt tcggcggcct cgatgacttg ggagatcttc
     2881 tcctcgacgt cggcgtgccg accgatcaga ccctcgccgt tgagcacgcc cagcccaccg
     2941 aggcggccca actcgatggc gaactccggc gacaccaacg cgtcggtggg atgcgacacc
     3001 accgggatct cgaaccggta ggcgtccatc tgccaagccg tcgacacgtc ctgcgacgac
     3061 cgggtgcgcc gcgacggcac gatgttgatg tcgtccagtt cataggtgcg acgggcggtt
     3121 ctgcccatgc caatttcaac catgtctcgc acgaccggct cctagtggct ggtgtagttc
     3181 ggtgcttcga cggtcatcgt gatgtcgtgc ggatggcttt ccttcaagcc cgcagccgtg
     3241 atctgcacga actgcgcctg ctgcaactgc tcgatggtcg ccgaaccggt gtagcccatg
     3301 gccgcgcgca ggccgccggt gagctggtgg atcactgtcg acagcgggcc gcggaatggc
     3361 acccggccct cgatgccttc cggcaccagc ttgtcctcgg agagcgcgtc gtcctggaag
     3421 tagcgatcct tcgaatagga cttggcgccg ccgcggccct gcatggcacc cagcgacccc
     3481 atgccgcggt agctcttgaa ctgtttgccg ttgacgaaga tcagctcccc cggcgattcc
     3541 gctgtgccgg ccagaagcga ccccagcatg gtggtggacg caccggcggc cagcgccttg
     3601 gcgatgtcac cggagtactg cagtccaccg tcggcgatga ccggcacgcc cttcggtgca
     3661 caggcggcga cggcctccag gatcgcggtg atctggggtg cgccgacgcc tgcgaccacg
     3721 cgcgtggtgc agatcgaccc gggcccgaca ccgaccttca ccgcgtcggc cccggcctgg
     3781 accagggcgg aggccgcggc acgggtcgcg acgttgccgc cgatcacgtc gacccgctca
     3841 ccgaccaagg tcttgagccg gtgcaccatg tcgagcacca accggttgtg cgcgtgcgcg
     3901 gtgtcgacga ccagcacgtc gactcctgca tcgacgagag tcatcgcgcg cgtccacgaa
     3961 tcgtcgccga caccgacggc cgcgccgacg agcagtcgac cgtcgctgtc cttggtggac
     4021 agcgggaact gttcggtctt gacgaagtcc ttgaccgtga tcagcccggt cagcttgccg
     4081 tggccgtcga cgatcggaag cttctcaatc ttgtagcggc gcaacagacc tagcgccgct
     4141 tcggcggaaa cacccgcttg cgcagtgatc agcggcgcct tcgtcatcac ctccgagacc
     4201 ggcttgctca tgtcgacttc gaagcgcatg tcgcggttgg tgatgatgcc gaccagggca
     4261 ccgtcggcgt ccaccaccgg caggccggag atccggaacc gggcgcacat ggcgtcgacc
     4321 tcggccaggg tgttgtccgg cgagcaggtg accgggtcgg tgaccatacc tg
//
LOCUS       DASRJA010000456         3205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1048519,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000456 DASRJA010000000
VERSION     DASRJA010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048519"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VFC01_20000"
     CDS             complement(<1..793)
                     /locus_tag="VFC01_20000"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZN81948.1"
                     /translation="MTIRVGVNGFGRIGRNFYRALAAQKAEGKSTDIEVIAVNDLTDN
                     ATLAHLLKFDSILGRYPEDVSLEGEDTIVVGNTKIKALEVKEGPAALPWGDLGVDVVV
                     ESTGIFTNAAKAKGHLDAGAKKVIISAPATDEDITIVLGVNDDKYDGSQNIISNASCT
                     TNCLGPLAKVLNDEFGIVKGLMTTIHAYTQDQNLQDGPHKDLRRARAAAINIVPTSTG
                     AAKAIGLVLPELKGKLDGYALRVPIPTGSVTDLTAELKKSATADEI"
     gene            complement(868..1578)
                     /locus_tag="VFC01_20005"
     CDS             complement(868..1578)
                     /locus_tag="VFC01_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN81949.1"
                     /translation="MSALRVGAAFPDPPFNGMPDNGGLDIDLMTAIAKAIGASVEFIP
                     YQGTDFNGIFDGLGTAYDCVIAGTTVTPERETKAKFVPPYLISGQSLAVDTTRLPHVK
                     SVDDLQGLVIGVQQGNTSQPMANRLVADGKAKTVRTYDYGTIRTALTDLTNGACDAFM
                     KLGPVLTELVKPIAGVEVVQRGISVENIAIAVPLDDQALLGRITVAQAELEDNGTLPK
                     LRQKWLGNPGADQSSAVH"
     gene            1652..2050
                     /locus_tag="VFC01_20010"
     CDS             1652..2050
                     /locus_tag="VFC01_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81950.1"
                     /translation="MEDPSTCGQGLSSRSPLPAKLGELLTALAQVLEAHMRALEPTDV
                     NARKELDAYATLVTGHRDIADRLAAISEQMAGYRDLPMAPHNEASMSDTVAHKAFERF
                     IRREQALAALLQDQLAQDEQMLRAMSGSPD"
     gene            2178..2435
                     /locus_tag="VFC01_20015"
     CDS             2178..2435
                     /locus_tag="VFC01_20015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81951.1"
                     /translation="MKNVIVGALLSGSVALAGLGLSIGTAQAFVPQPDPPGKVAPSQN
                     VNPGEIRGFDPQPDPPGAKPSRQLNPGVIHGFNPQPEPPVG"
     gene            complement(2463..>3205)
                     /gene="whiA"
                     /locus_tag="VFC01_20020"
     CDS             complement(2463..>3205)
                     /gene="whiA"
                     /locus_tag="VFC01_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HZN81952.1"
                     /translation="KSTRYVVRVAKDGEALARQTGLLDLRGRPVRGLPAQVVGGSVAD
                     AEAAWRGAFLAHGSLTEPGRSSALEVSCPGPEAALALVGAARRLGVSAKAREVRGSDR
                     VVVRDGEAIGALLTRMGAQDTRLVWEERRMRREVRATANRLANFDDANLRRSARAAVA
                     AAARVERALEILGDTVPDHLAAAGKLRVEHRQASLEELGRLADPPMTKDAVAGRIRRL
                     LSMADRKAKQEGIPDTESAVTPDLLEDA"
BASE COUNT          510 a         1039 c         1056 g          600 t
ORIGIN      
        1 tgatctcgtc ggctgtcgcc gacttcttca gctcggcggt caggtcggtg accgagcccg
       61 tggggatcgg cacccgcagg gcgtagccgt ccagcttgcc cttcagctcg ggaagcacca
      121 gcccgatggc ctttgcggcg ccggtcgagg tcggcacgat gttgatcgcg gcggcgcggg
      181 cgcggcgcag gtccttgtgc gggccgtcct gaaggttctg gtcctgcgtg tacgcatgga
      241 tcgtcgtcat caaacctttg acgatgccga actcgtcgtt gagcaccttg gccagcgggc
      301 cgaggcagtt cgtggtgcac gaggcgttgg agatgatgtt ctggctgccg tcgtacttgt
      361 cgtcgttaac gccgagcacg atcgtgatgt cctcgtcggt ggcaggcgcg gaaatgatga
      421 ccttcttggc gccagcgtcg aggtggccct tggccttggc ggcgttggtg aagatgccgg
      481 tggactcgac gaccacgtcg acgcccaggt caccccaggg aagcgcggcg gggccctcct
      541 tgacctcgag cgccttgatc ttggtgttgc cgacgacgat ggtgtcctcg ccctcaaggc
      601 tgacatcctc ggggtagcgg ccgaggatcg agtcgaattt cagcaggtgc gccagcgtgg
      661 cgttgtcggt gaggtcgttg accgcgatca cctcgatgtc ggtgctcttg ccctcggcct
      721 tctgtgccgc cagtgctcgg tagaagttgc gtccgatgcg gccgaagccg ttcacgccga
      781 cccggatggt cacttgtctc tccctgttag ttgccttgtt tctcggcgcc cagcctagtg
      841 gtgtgatact cgccatgcgc gggctggtca gtggacggcc gagctctggt ccgcgccggg
      901 gttgcccagc catttctgcc tgagcttcgg caaagtcccg ttgtcttcca gctcggcttg
      961 cgcgacggtg atgcggccga gcagtgcctg gtcatcgagc gggacggcga tggcgatgtt
     1021 ctccacggag atgccgcgct gcacgacctc gaccccggcg atcggcttga ccagttcggt
     1081 cagcaccggc cccagcttca tgaaggcatc gcaggcgccg ttggtcaggt cggtcagcgc
     1141 ggtgcggatg gtgccgtagt cgtaggtgcg aacggtcttg gctttgccgt cggcgacgag
     1201 gcggtttgcc atcggctggc tggtgttgcc ctgctgcaca ccgatgacga ggccttggag
     1261 gtcgtcgacg gacttcacgt ggggcagcct ggtggtgtcg acggccagcg actggccgga
     1321 gatgaggtag ggcgggacga acttcgcctt ggtctcgcgt tcgggtgtga cggttgtgcc
     1381 cgcgatcacg cagtcatatg cggtgcccag gccgtcgaag atgccgttga agtcggttcc
     1441 ctggtacggg atgaattcga cggaggcacc aattgctttg gcgattgcgg tcattagatc
     1501 gatgtcgagg ccaccgttgt ccggcatgcc attgaacggc ggatccggaa atgcggcacc
     1561 aacccgaagt gcgctcacgg cgtcaactgt atgtccggcg atggttccgg cggccgtttg
     1621 agtagcgcac agagcgggga gacttccgga tatggaagac ccatcgacct gcggccaagg
     1681 tttatcgagt cgatcgcctc tgcctgccaa gctcggggag ttgctcacgg cgctcgcaca
     1741 ggtcctcgaa gcacacatga gggcactcga acccacagac gtcaacgcga gaaaagagct
     1801 cgacgcctac gcgacgttgg tgacggggca tcgggatatc gccgaccgat tggcagcgat
     1861 ctctgagcaa atggccgggt accgggatct gccaatggca ccgcacaacg aagcttcgat
     1921 gtcagacacg gtggctcaca aagcctttga gagatttatc cggcgtgagc aggcgcttgc
     1981 cgcattgctg caggaccagt tagcgcagga cgagcagatg cttcgtgcga tgtcgggatc
     2041 ccctgactga tttcgaggtc atggtcaccc tcggattaac gcgccaagaa tgtgccaagg
     2101 ccggccacgg atgctgactc tgactcacaa cgggtaaccc gggccgccgg gaacgcttcg
     2161 tatgcaagga gaacggcatg aagaacgtca tcgtcggggc gctgctgtcg ggcagcgtcg
     2221 cgctggccgg actggggctg agtatcggca ccgcccaggc attcgtgccg cagcccgatc
     2281 cacccggcaa ggtcgcaccg tcgcagaatg tgaatccggg cgaaattcgc ggcttcgacc
     2341 cgcaaccaga cccgccgggg gctaaaccgt cccggcagct gaatccgggc gtcatccatg
     2401 ggttcaaccc gcaaccggaa ccgcccgtcg gctaacggga ttgatgcatc tgccgtggaa
     2461 agctaggcgt cttcgagcaa gtctggcgtg acggcggact cggtgtcggg aatgccttct
     2521 tgtttggcct tgcggtcggc catcgacagc agccgccgaa tccggcctgc cacagcatct
     2581 ttcgtcatcg gcgggtcagc caggcgaccc agttcctcca gcgacgcctg ccggtgctcg
     2641 acacgcagct tgcccgcggc ggccagatga tctggcacgg tgtcgccgag gatctccaat
     2701 gcccgctcca cccgggccgc tgccgccacc gctgcccggg ccgatcgccg caggttcgcg
     2761 tcgtcgaagt tggccagccg attcgcggtc gcccgcacct cacgacgcat ccgtcgctct
     2821 tcccaaacca gccgggtgtc ctgcgcgccc atccgcgtca gtaacgcacc gatagcttcg
     2881 ccgtcgcgca ccaccacgcg gtcgctgcct cgcacctcac gcgccttcgc gctgaccccc
     2941 agccgccgcg ccgcgccgac gagcgccagc gccgcctccg gtccagggca gctcacctcc
     3001 agcgccgacg atcgacccgg ctcggtcagc gatccgtgcg ccaaaaacgc tccgcgccaa
     3061 gcagcttcgg catcggcaac gctgccgccg accacctgcg cgggcaggcc gcgcaccggt
     3121 cggccacgca gatcgagcag tcccgtctgg cgcgccagcg cttctccgtc cttcgctacc
     3181 cgcaccacgt aacgcgtgct cttgc
//
LOCUS       DASRJA010000457        10354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3396530,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000457 DASRJA010000000
VERSION     DASRJA010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10354
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3396530"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFC01_20025"
     CDS             complement(<1..621)
                     /locus_tag="VFC01_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZN81953.1"
                     /translation="MAHRKKSRGHRHHGAAAGLSGPAVTHAQSRVLHSVDTHPPSTNE
                     GSLWGRRRVLLLNSTYEPLTALPMRRAVIMLMCGKASVVHDDPNGPVIHSATRSIVVP
                     SVIRLHSYVRVPYRARIPMTRAALMHRDRFRCAYCGGKADTVDHVVPRSRGGDHTWEN
                     CVAACSTCNHRKADRLLAELGWTLRSVPVPPKGQHWRLLSSVKELDP"
     gene            741..1145
                     /locus_tag="VFC01_20030"
     CDS             741..1145
                     /locus_tag="VFC01_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HZN81954.1"
                     /translation="MERVTQPQRSYYEEVGGHDTFNAIVSRFYQLVREDEILRPLYPE
                     DELDAAEERLRMFLEQYWGGPRTYSDRRGHPRLRMRHAPFKIGYIERDAWLRCMHTAV
                     AEIDSQTLDDEHRKELIAYLEMAAHSMVNSPF"
     gene            1145..2710
                     /locus_tag="VFC01_20035"
     CDS             1145..2710
                     /locus_tag="VFC01_20035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HZN81955.1"
                     /translation="MTTPWWSHALFYQVYPRSFRDSDGDGVGDLDGVTAKLDYLKTLG
                     IDAIWLNPVMVSPMADNGYDVADPRDVDPLFGGIEALDRLTDAAHRLGMKVTMDLVPN
                     HTSSQHPWFQAALAASPGSDQRERYIFRDGIGPGGVHPPNNWVSIFGGPAWTRVIEAD
                     GNPGQWYLHLFDAEQPDLNWENPEVFEDLEKTLRFWLERGIDGFRIDVAHGMAKPPGL
                     PDMETITAELLLADMDDDPRFNNEGVHEIHRQIRRVFNDYPDAVAIGEVWVYDNEQFS
                     RYVRHDELHLGFNFRLLRAKFDATDIRGAIENSMTAAAMVDAVPTWTLANHDVDREVT
                     RYGDGAIGLARARAMALVMLALPGAVFIYNGEELGLPNVELPDEALRDPVWERSGHTR
                     RGRDASRVPVPWDGDAPPFGFSSSSDTWLPLPPGWAALTVAKQLGDPDSMLSFFRRVF
                     ELRGSRPEFGGATLEWLDSPAGTVVFRVSSGLVCALNAGRRSMALPDGEVLLASGALV
                     DGKLPPNTAAWLV"
     gene            complement(2789..3469)
                     /locus_tag="VFC01_20040"
     CDS             complement(2789..3469)
                     /locus_tag="VFC01_20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81956.1"
                     /translation="MTEPERGIWLDDVTHREDLTTFAERALRLDDAAVVRLRERAGGL
                     VVAWVATGFDVLASRVVRGRIKPADLSAGADALSAGLTAMDRSGYVEPGFPMDSAWRG
                     ALPPETGFVHLDDVPARAVLDLAQRGVALAKEHSSAHGPPASLLDQEVVAVSSGDASV
                     GIPMRCVFALTAMGFLPQSGAAEELDPGTVAADEIVRVRVLPAWLRIDARFGSVYRRR
                     GDPALVLR"
     gene            complement(3466..3876)
                     /locus_tag="VFC01_20045"
     CDS             complement(3466..3876)
                     /locus_tag="VFC01_20045"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZN81957.1"
                     /translation="MTGFVAPVHVRWSDIDMYQHINHATMVTILEEARIPFLREPFES
                     VIEEIGLLIHEVKILYKGQLRLIDSPLQVTMWSKRVRAVDFTIGYEVRSVRRGADSKP
                     SVIAETQLAAVHIKEQRLERLSSVQREYLQHYLR"
     gene            complement(3873..8726)
                     /locus_tag="VFC01_20050"
     CDS             complement(3873..8726)
                     /locus_tag="VFC01_20050"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HZN81958.1"
                     /translation="MTVNPGTGTGVGGSTDSPPLSQAAIARLARAYMSTYRGPHGDAP
                     GADSAVTAPLQRTVAGMVSPALVDAHLRLGRHRSFGDTRVASYAGDDAGGFGPALQIV
                     TDNAAMLMDSVTVLLHRLGVAYTAIMNPVFRARRGPSGDLLAIEPASDAPFGDGIDEA
                     WIHVQLTESVDPKAVEEVKRLLPSVLADARQVGLDSTSMAAMLRSLAAELDFDSEGRF
                     PGSDRKDVAALLRWLADGHFVLLGYQRCQVRDGQSSVDPSSRLGVLRLRTDVLPQLTT
                     SDDLLVLAQATMPSYLRYGAYPYIVVVREHARGEAIEHRFVGLFTVAAMNANVLEIPL
                     VSRRVNDALAMAHRDPSHPGQLLLDIIQTIPRSELFALSARELLDMAMAVVDLGSRRR
                     TLLFLRADQLAHFVSCLVYLPRDRYTTAVRLAMQDILVRELGGVSIDYAARVSESPWA
                     VVHFTVRLPEGRRPQDIDVSLDNESRIQDLLTEAARTWGDRLIGAVKTGSINQAKAEH
                     YSAAFPEVYKQAIAPLDAITDIAIIEELQDNTVRLVLGDRGPDGVAQLTWYLGGRSAS
                     LSQLLPMLQSMGVVALEERPFTVTRPDGLPIWIYQFKISPHPDIPTPPAGPERAAMAD
                     RFADAVTAIWHGRAEIDRFNELVLRAGLTWQQVVVLRAYAKYLRQAGFPYSQSHIETV
                     VNDNAGTARSLIELFQALFDPTPPDEARRSAAQTAAAAVAADIDALVSLDTDRVLRAF
                     ASMIQATLRTNYFVSNPDSARQQNVLSVKLNPRLIDELPLPRPKFEIFVYSPRVEGVH
                     LRFGHVARGGLRWSDRREDFRTEILGLVKAQAVKNAVIVPVGAKGGFVVKKPPKPTGD
                     AAADRDAHRNEGVACYKLFIAGLLDLTDNVDKATGDVVTPTDVVRRDSDDAYLVVAAD
                     KGTATFSDIANEVAASYGFWLGDAFASGGSVGYDHKAMGITAKGAWEAVKRHFREMGV
                     DTQTEDFTVVGVGDMSGDVFGNGMLLSQHIRLLAAFDHRHIFIDPDPDAAASWDERKR
                     LFELPRSSWEDYNASLISAGGGVYSREQKSIPISPQARAALGIAESVEEMTPPALMKA
                     ILKAPADLLFNGGIGTYIKAETESDADVGDRANDPVRVTGNQVRAKVIGEGGNLGVTS
                     LGRIEFCLAGGRCNTDALDNSAGVDCSDHEVNIKILIDSLVTAGKVKAEDRTALLVSM
                     TDEVGRLVLADNEDQNDLMGTSRANAASLLPVHARMIKDFVKDRGLNRELEALPSEKE
                     IGRRTEAGIGLTSPELATLMAHVKLALKDDLLASELPDQEVFASRLPWYFPSNLREQF
                     AGDIRQHQLRREIVTTMLVNDLVDTSGITFAYRVSEDVGVGPVDAVRSYVAANAIFRI
                     SEVWREIRAANVSVAVSDRMTLDLRRLVDRSGRWLLNYRPQPLAVGAEINRFAEKVRV
                     LTPRMSEWLRGDDKAIVDKEAREFSSHGVSEELAHMVATGLYQYSLLDVIDIADIIDR
                     DPGEVADTYFALMDRLGTDGLLTAVSRLVRDDRWHSLARLAIRDDIYGSIRALCFDVL
                     AVGEPDESGEEKIAEWETTNSSRVSRAQRTLTEIYESGELDLATLSVAARQLRSMTRT
                     SGTGTTG"
     gene            complement(8779..>10354)
                     /gene="ettA"
                     /locus_tag="VFC01_20055"
     CDS             complement(8779..>10354)
                     /gene="ettA"
                     /locus_tag="VFC01_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HZN81959.1"
                     /translation="IGVVGPNGAGKSSVLRIMAGLDIPNNGDALLQPGATVGILQQEP
                     QLDETKTVRENVEEGVAIKAKLNRYNEVAELMATDYSDELMEEMGHLQEELDAADAWD
                     IDSQLEQAMDALRCPPADEPVTHLSGGEKRRVALCKLLLSKPDLLLLDEPTNHLDAES
                     VLWLEQHLAQYKGAILAVTHDRYFLDNVAEWILELDRGRAYPYEGNYSTYLEKKAERI
                     AVQGRKDAKLQKRLRDELAWVRSGVKARQAKSKARLQRYEEMATEAEKTRKLDFEEIQ
                     IPIGPRLGSVVVEVEHLDKGFEGRPLIKDLSFTLPRNGIVGVIGPNGVGKTTLFKTIV
                     GLEQPDSGTVRVGDTVKLSYVDQSRAGIDPKKTVWEVVSDKLDYIEVGQNEIPSRAYV
                     SAFGFKGPDQQKPAGVLSGGERNRLNLALTLKEGGNLILLDEPTNDLDVETLSSLENA
                     LDNFPGCAVVISHDRWFLDRTCTHILAWEGDDDNEAKWFWFEGNFAAYEENKVERLGI
                     EAARPHRVTHRRLTRD"
BASE COUNT         1701 a         3481 c         3375 g         1797 t
ORIGIN      
        1 cgggtcgagt tccttgaccg acgacagcag ccgccaatgc tgtcccttcg gcggcacagg
       61 caccgaacgc agcgtccagc ccagctcggc gagcaaccgg tcggccttgc ggtggttgca
      121 ggtcgagcac gcagccacgc agttctccca ggtgtggtcg ccaccgcggc tgcgcggcac
      181 cacgtggtcg acggtgtcgg ccttgccgcc gcagtaggcg cagcggaacc ggtcgcggtg
      241 catcagcgcc gcacgcgtca tcgggatgcg cgcccggtaa ggcactcgga cgtaggaatg
      301 cagccggatc actgacggca ccacgatcga ccgggtcgcg gagtggatca ccggcccgtt
      361 cgggtcgtcg tgcaccacgc ttgctttgcc gcacatcagc atgatgaccg cccgccgcat
      421 gggcagagcg gtcaacggtt cgtaggtgga attcaatagc agcacgcgcc ggcggcccca
      481 gagggagcct tcgttcgtac tagggggatg ggtatcgacg ctgtgcaaga cgcgcgactg
      541 cgcatgcgtt accgcgggcc cagataagcc cgccgcggcc ccgtggtggc ggtggccgcg
      601 gctcttcttg cgatgcgcca tacgtcctcc gaccgacagt ccaccacgga ttggggacaa
      661 acgcacgaca attctgcctg tgttcgctgg tcagtcccgt gaacacctgg tgacgtagcc
      721 ggttaccggc cacatggaca atggagcgcg tgacacagcc gcagcgctcg tattacgaag
      781 aagtcggtgg ccacgacacg ttcaatgcga tcgtgtcgcg gttctaccag ctggtccgcg
      841 aggacgagat cctgcgtccg ctctatccgg aggatgagct cgacgccgcg gaagagcggc
      901 tgcgaatgtt cctcgagcag tactggggcg gcccgcggac ctactccgac cgtcgtggcc
      961 atccccggct gcggatgcgg catgcgccgt tcaagatcgg ctacattgag cgcgacgcgt
     1021 ggctgcgctg tatgcacacg gccgtcgcgg agatcgactc gcagacgctc gacgacgagc
     1081 atcgcaagga actgatcgcc tacctggaga tggcggctca ttcgatggtc aactcaccgt
     1141 tctgatgaca acaccctggt ggtcgcacgc gctgttctat caggtctatc cgcggtcgtt
     1201 ccgggatagc gacggcgacg gcgtcggtga cctcgacggc gtgacggcga agctggacta
     1261 cctcaagacg ctcggcatcg acgcaatctg gctcaatccg gtgatggtct cgccgatggc
     1321 cgacaacggc tacgacgtgg cggacccccg cgacgtcgac ccgctgttcg gcggcatcga
     1381 ggcgcttgac cggctgaccg acgccgcgca ccggttgggc atgaaagtga cgatggacct
     1441 ggtgcccaac cacaccagtt cgcagcatcc gtggtttcag gcggcgttgg ccgcaagccc
     1501 gggcagcgac cagcgcgagc gctacatctt ccgcgacggc atcggccccg gcggcgtcca
     1561 tccgccgaac aactgggtgt cgatcttcgg cggccccgcg tggacccgcg tcatcgaggc
     1621 cgacggcaat ccaggccagt ggtacctgca cctgttcgac gccgagcagc cggacttgaa
     1681 ctgggagaac cccgaggtct tcgaggacct ggagaagacg ttgcggttct ggctggagcg
     1741 cggcatcgac ggcttccgca tcgacgtcgc ccacggcatg gccaagccac cgggcttgcc
     1801 ggacatggag accatcacgg ccgagttgct gctggccgac atggatgacg acccgcggtt
     1861 caacaacgag ggcgtgcacg agatccaccg ccagatccgc cgggtgttca acgactaccc
     1921 ggacgcggtc gccatcggcg aggtgtgggt gtacgacaac gagcagttct cccgatacgt
     1981 gcgccacgac gagctgcacc ttgggttcaa cttccggctg ctgcgggcga agttcgatgc
     2041 gacggacatc cgcggggcca tcgagaattc gatgacggcc gcggcgatgg tggacgcggt
     2101 accgacctgg acgcttgcca atcacgacgt cgatcgcgag gtgacccgct acggcgacgg
     2161 ggcaatcggc ttggcccgcg cgcgggcgat ggcgttggtg atgctggccc tgccaggcgc
     2221 ggtgttcatc tacaacggcg aggagctcgg cctgccgaac gtcgagctgc ccgacgaggc
     2281 cctgcgcgac ccggtgtggg agcggtccgg ccacaccagg cgcggccgcg acgcgtcgcg
     2341 ggtgccggtg ccgtgggacg gcgacgctcc cccgttcggc ttctcttcca gttctgacac
     2401 gtggctgccg ctaccgccag gatgggcggc actgacagtg gcgaagcagc tcggcgaccc
     2461 cgactctatg ctgtcattct tccggcgcgt attcgaattg cgcggcagcc gtccggaatt
     2521 cggcggcgcc accctcgaat ggctcgactc ccccgctggc actgtggtgt tccgagtcag
     2581 cagcggcttg gtgtgtgcgc tcaacgccgg ccggcgttcg atggccctgc ccgacggcga
     2641 ggtgttgttg gccagcggcg cgttggtgga tggcaagctg ccgccgaaca ccgccgcctg
     2701 gctggtgtag ttctccgcgc gagcagacgc aaaacttccc atttcacgcc gagaatgggc
     2761 atgtttgcgt ctgctcgcgc tcaatgagtc acctcaagac cagggcgggg tcgccgcggc
     2821 ggcgatacac cgaaccgaag cgggcgtcga tgcgcaacca cgctggcagc acgcgcaccc
     2881 gaacgatctc gtcggcagcg acagtgcctg ggtccaactc ctccgcagcg cccgactgcg
     2941 gcaggaaacc cattgcagtc aaggcgaata cacaccgcat cgggatgccg acactggcgt
     3001 cgcccgaact gacggccacc acctcctgat ccagcagcga cgcgggtggc ccatgcgcgc
     3061 tcgagtgctc cttcgccagc gcgacgccgc gctgcgctag gtcgagcacc gcacgcgctg
     3121 gcacgtcgtc gaggtggacg aagcctgtct cgggcggaag cgcgccgcgc cacgcggaat
     3181 ccatcgggaa tccgggttcg acgtaaccgc tgcggtccat cgccgtcagc ccagcggaca
     3241 gcgcatcggc tcctgcgctc agatcggccg gcttgatgcg cccacgcaca acccggctgg
     3301 ccagcacgtc gaaacccgtt gccacccagg cgaccacgag tcccccggcc cgctcccgca
     3361 gccgaacgac cgcggcatcg tcgagccgca atgcccgttc ggcgaacgtc gtcaggtcct
     3421 cacggtgtgt gacgtcgtcg agccagatac cgcgttcggg ttcagtcatc gaaggtagtg
     3481 ctgcagatac tcacgctgga cggacgaaag ccgctccagc cgttgctcct tgatgtggac
     3541 ggcggcaagc tgcgtctcag cgatcaccga cggtttggag tccgcgcccc tcctcaccga
     3601 gcgcacctca tagccgatcg tgaagtcgac ggcccgcacc cgcttcgacc acatcgtcac
     3661 ctgcagcggc gaatcaatca ggcgcagctg acccttgtag aggatcttga cctcgtggat
     3721 cagcagcccg atctcctcga tcacagactc gaacggctca cgcagaaaag ggattctggc
     3781 ctcttcgagg atggtgacca tggtggcgtg gttgatgtgc tgatacatgt caatgtccga
     3841 ccaccgcaca tgcaccggcg cgacaaagcc agtcacccag ttgtccccgt tccactcgtt
     3901 ctcgtcatgc tgcgcagctg tcgcgcggcg accgacagcg tcgccaggtc gagctcgcca
     3961 ctttcataga tctcggtcag cgtgcgctgc gcccggctca cacgggagct gttggtcgtc
     4021 tcccactcgg cgatcttctc ctcgccactc tcgtccggct ccccgaccgc cagcacatcg
     4081 aagcacagcg ccctaatcga accgtagatg tcgtcgcgaa tcgccaagcg ggccaacgaa
     4141 tgccagcgat cgtcgcgcac gagtcgggac accgcggtca gcaagccatc ggtgccgagg
     4201 cggtccatca gcgcgaaata agtgtcggca acctcgcccg ggtcacggtc gatgatgtcg
     4261 gcgatgtcga tcacgtcgag caggctgtac tggtacagcc cagtggcgac catatgcgcc
     4321 agttcctccg agacaccgtg cgaactgaac tcgcgggctt ccttgtcgac gatggctttg
     4381 tcatcgccac gcagccactc cgacatccgc ggtgtcagca cacggacctt ttccgcgaac
     4441 cggttgatct cggcgcccac cgccagcggc tgcggccgat agttgagcag ccagcgtccc
     4501 gagcgatcga ccagccggcg cagatccaac gtcatccggt ccgacacggc caccgagaca
     4561 ttcgccgccc ggatctcccg ccacacctca ctgatccgga agatcgcgtt cgccgcgacg
     4621 tagctgcgca ctgcgtcgac cggtccgacg ccgacgtctt cggagacccg gtatgcgaag
     4681 gtgatgccgc tggtgtcgac gagatcgttg accagcatgg tcgtgacgat ttcccggcgc
     4741 agctgatgtt ggcggatatc gccggcgaac tgctcgcgca ggttggacgg gaagtaccac
     4801 ggcagccgag aggcgaacac ctcctgatcg ggcagctcgc tggcgagcag atcgtccttc
     4861 agcgccagct tcacatgggc catcagcgtc gcgagttcgg gtgaggtgag cccgattcca
     4921 gcttctgtac ggcgcccgat ctccttctcg gacggcaacg cctcgagctc gcggttgagc
     4981 ccgcggtcct tgacgaaatc cttgatcatc cgagcgtgca ccggtagcag gctggccgcg
     5041 ttggcgcggc tggtgcccat caggtcgttt tgatcctcgt tgtcggccaa caccagtcgg
     5101 ccgacctcgt cggtcatcga taccagcagg gcggtccggt cctcggcttt gaccttgccg
     5161 gcagtgacca gtgagtcgat caggatcttg atgttgacct catggtcgga gcagtcgaca
     5221 ccggccgagt tgtccaatgc gtcggtgttg cagcggccgc cagcaaggca gaactcgatg
     5281 cggcccagcg aggtgacgcc caagttgccg ccttcaccga tcaccttggc gcgcacctga
     5341 tttccggtca ctcggaccgg gtcgttggcg cggtcaccga cgtcggcatc cgattccgtc
     5401 tctgccttga tgtacgtgcc gatgccgccg ttgaacagca ggtcggccgg ggccttcagg
     5461 attgccttca tcaaagccgg gggcgtcatc tcctcgacgc tctcggcgat gcccaaggcg
     5521 gcgcgggcct gcgggctgat cgggatggac ttctgttcgc ggctgtacac gccgccgccc
     5581 gcgctgatca gcgacgcgtt gtaatcctcc cagctggacc gcggcagctc gaagagtcgt
     5641 ttgcgctcat cccacgacgc ggccgcgtcc gggtcggggt cgatgaagat gtgccgatga
     5701 tcgaacgctg ccaatagccg gatgtgttgg gacagcagca tgccgttgcc gaagacgtcg
     5761 ccgctcatat cgccgacgcc gacgaccgtg aagtcctcgg tttgggtgtc gacgcccatc
     5821 tcgcggaagt gccgcttgac ggcctcccac gcgcccttgg cggtgatgcc catcgccttg
     5881 tggtcgtagc cgaccgaacc gccggatgcg aacgcgtcgc cgagccagaa cccgtacgac
     5941 gcggccacct cgttggcgat gtcggagaac gtggcagtgc ccttatcggc ggccaccacg
     6001 aggtatgcgt cgtcgctgtc gcgccgcacc acatcggtcg gggtgacgac gtcgccggtg
     6061 gccttgtcga cgttgtcggt gaggtcgagc agcccggcga tgaacagctt gtagcacgcc
     6121 acaccttcat ttcggtgcgc gtcgcggtcg gcggcggcat cgccggtggg cttcgggggc
     6181 ttcttgacga cgaagccgcc cttggctccg accgggacga tgaccgcgtt cttcaccgcc
     6241 tgcgccttga cgaggccgag aatctcggtg cggaagtcct cccggcggtc cgaccatcga
     6301 agaccgccgc gcgccacgtg accgaatcgc aggtgcacgc cctcgacccg cggcgagtac
     6361 acgaagatct cgaacttggg ccggggcaac ggcagttcgt cgatcaggcg ggggttgagt
     6421 ttgaccgaca gcacgttctg ctggcgcgcc gagtccgggt tgctgacaaa gtaattggtc
     6481 cgcagcgtgg cctggatcat cgatgcgaac gcccgcagta cccggtcggt gtcgaggctg
     6541 acgagtgcgt cgatgtccgc cgcgacggcg gcggcggccg tctgcgcggc actgcgccgc
     6601 gcctcgtctg ggggcgtcgg gtcgaacaac gcctggaaca actcgatcaa cgatcgcgcg
     6661 gtgcctgcat tgtcgttgac cacagtttcg atgtgcgact ggctgtacgg aaaacccgcc
     6721 tgccgcaggt atttcgcgta tgcgcgcaac acaaccacct gttgccaggt caggcccgcc
     6781 cgcagcacca actcgttgaa gcgatcgatc tcggcgcgac cgtgccagat cgcggtgacg
     6841 gcgtcggcaa accggtcggc catcgccgcg cgttccgggc cggccggcgg cgtcgggatg
     6901 tctggatgcg gcgaaatctt gaactggtag atccagatcg gcaatccgtc gggccgggtc
     6961 actgtgaacg ggcgctcctc gagcgctacc actcccatcg actgcagcat cggcagcagc
     7021 tggctcagcg acgccgaccg tccacccaga taccacgtga gctgcgcgac cccgtcggga
     7081 ccgcggtcgc caagcaccag tctgaccgta ttgtcttgca gctcttcgat gatcgcgatg
     7141 tcggtgatcg cgtccagcgg cgcgatcgcc tgcttgtaga cctccggaaa cgccgccgaa
     7201 tagtgctcgg ccttggcctg gttgatcgag cccgtcttca ccgcgccaat cagccggtcc
     7261 ccccaggtcc tggcggcctc ggtgagcagg tcctggattc gggactcgtt atcgagcgag
     7321 acgtcgatgt cctgcggacg acggccctcg ggcagccgca ccgtgaaatg cacgacggcc
     7381 catggtgatt cgctgacgcg cgcggcatag tcgatgctca ccccgcccag ttcgcggacc
     7441 aggatgtcct gcatcgccag acgcaccgcg gtggtgtacc ggtcgcgcgg caggtacacc
     7501 aggcatgaca cgaagtgcgc gagttgatcg gcgcgcagga acagcagcgt tcgacgccgt
     7561 gagccgagat cgaccaccgc catcgccatg tccaggagtt ctctcgcgga cagcgcgaac
     7621 agctccgaac gcgggatggt ctggatgatg tccagcagca gctggccggg atggctcggg
     7681 tctcggtgcg ccatcgcgag cgcgtcgttg acccgtcgcg aaaccaacgg aatttcaagc
     7741 acattggcgt tcatggcggc caccgtgaac aacccgacga agcggtgctc gatcgcctct
     7801 cctctggcgt gttcgcggac caccacgatg tacgggtacg cgccgtagcg caggtagctc
     7861 ggcatggtgg cctgcgccag cactaacagg tcgtcgctgg tggtgagctg cggaagcacg
     7921 tcggtgcgca gccggagcac accgagacgg ctggacgggt cgaccgagga ctggccgtcg
     7981 cggacctgac agcgctggta gcccagcagc acgaagtggc cgtcggccag ccagcgcagc
     8041 agcgcggcga catccttgcg gtcggagccg ggaaacctgc cctcggaatc aaagtcgagt
     8101 tcggccgcca ggctgcgcag catggccgcc atcgaggtgg agtccagccc cacctgacgg
     8161 gcgtcggcga gaacgctggg cagcaggcgc ttgacctcct ccaccgcctt ggggtcgacc
     8221 gactccgtca gctggacgtg gatccaggcc tcgtcgatac cgtccccgaa cggggcgtcg
     8281 gatgccggct cgattgccag caggtcgccg ctgggccccc ggcgtgcccg gaacaccgga
     8341 ttcatgatcg ctgtgtaggc caccccgagc cggtgcagca gcacggtgac ggagtccatc
     8401 aacatcgcgg cgttatcggt gacgatctgc aacgccgggc cgaacccgcc cgcgtcgtcg
     8461 ccggcgtagc tcgcgacccg ggtgtccccg aaggaccggt gccgaccgag tcgaaggtgt
     8521 gcgtccacca gtgccggcga caccatgccc gccaccgtcc gctgcagggg cgcggtgacc
     8581 gccgagtcgg cacccggcgc gtccccgtgc gggccgcgat aggtcgacat gtaggcccgg
     8641 gcgagcctgg cgatggcggc ctggctcagc ggcggcgagt cggtcgatcc gccgacacct
     8701 gtgccagtcc ccggattcac cgtcatcgca ccgctcctac tcgcacccgc gtgccgacgc
     8761 tggcccgcac aggcgacatt agtcgcgcgt gagccggcgg tgggtcaccc tgtgcggacg
     8821 ggccgcttcg ataccaagtc gttccacttt gttctcttcg taggcggcga agttgccctc
     8881 gaaccagaac catttggcct cgttgtcgtc gtcgccctcc cacgccagga tgtgcgtgca
     8941 cgtgcggtcg aggaaccacc ggtcgtggga aatcaccacc gcgcagccgg ggaagttgtc
     9001 cagcgcgttc tccagcgagc tcaatgtttc cacgtccagg tcgttggtcg gctcgtcgag
     9061 caggatcagg ttgccgccct ctttgagggt gagcgccagg ttgagccggt tgcgttcacc
     9121 gccggacagc acgcctgcgg gtttctgctg gtccggcccc ttgaaaccga acgccgagac
     9181 gtaggcgcgc gaagggatct cgttctggcc gacctcgatg tagtccaact tgtcggagac
     9241 gacctcccag accgttttct tggggtcgat cccggcccgg ctctgatcga cgtagctcag
     9301 cttgacggtg tcgccgaccc tgacggtgcc gctgtccggc tgctcaaggc cgacgatggt
     9361 cttgaacagc gtggtcttgc cgacaccgtt cgggccgatg acgccgacga tgccgttgcg
     9421 cggcagcgtg aacgacagat ccttgatcag cggccgaccc tcgaagccct tgtcgaggtg
     9481 ctcgacctcg acaacgacac tgcccaggcg tggaccgatc gggatctgga tttcctcgaa
     9541 gtcgagcttc cgcgtcttct cggcctccgt cgccatctcc tcgtagcgct gcaggcgggc
     9601 cttgctcttg gcctgccgag cctttacccc cgagcgcacc cacgcgagct catccctgag
     9661 tcgcttctgc agcttggcgt ccttgcggcc ctgcaccgcg atgcgttcgg ccttcttctc
     9721 caagtaagtg gagtaattgc cctcgtacgg gtaggcgcgg ccgcggtcga gttcgagaat
     9781 ccactcggcc acgttgtcca ggaagtaccg gtcgtgggtg acggccagga tcgcgccctt
     9841 gtactgcgcg aggtgctgtt cgagccacaa aacgctctcg gcgtcgaggt ggttcgtcgg
     9901 ttcgtcgagc agcagtagat cgggcttcga cagcagcagc ttgcagagcg ccacccggcg
     9961 cttctcacca ccggacaggt gtgtgacggg ttcgtcggcc ggcgggcagc ggagcgcgtc
    10021 catcgcctgc tccagctggg aatcgatgtc ccacgcgtcg gccgcgtcca gctcctcctg
    10081 cagatggccc atctcctcca tcagctcgtc ggagtagtcg gtggccatca gctcggcgac
    10141 ctcgttgtag cggttgagtt tggccttgat ggccacgcct tcctcgacgt tctcgcgcac
    10201 cgtctttgtc tcatcgagct ggggctcctg ctgcaggatg ccgaccgtcg caccgggctg
    10261 taagagggcg tcgccgttgt tggggatgtc cagtccggcc atgatccgca agacgctcga
    10321 cttgccggcc ccgttcgggc cgacgacgcc gatc
//
LOCUS       DASRJA010000458         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3676267,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000458 DASRJA010000000
VERSION     DASRJA010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3676267"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFC01_20060"
     CDS             complement(<1..736)
                     /locus_tag="VFC01_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011597562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZN81960.1"
                     /translation="MRGFFYGLFVPHSHDPADSIDDALEASTAGIRAVKISLILLAIT
                     ATLQIVVVIVSGSMALLADTIHNFSDAMTAVPLWVAFVLSRRAATRRYTYGYGRVEDL
                     AGLFIVTMITSSAVLAAYQSIRRLFEPQPLTNLGWVLAAGLIGFAGNELVAVYRIRVG
                     RRIRSAALVADGLHARTDGFTSLAVVLGVIGVWAGFPLADPVVGLLISTVIFILLWGT
                     AGNIGRRLLDGVDPSLVDRAEHSIRSV"
     gene            746..907
                     /locus_tag="VFC01_20065"
     CDS             746..907
                     /locus_tag="VFC01_20065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81961.1"
                     /translation="MLMTVIMVMSVPATVLVVMALIVVVAILDVDRVAVLTVNMLSAV
                     ARHRCRIGV"
     gene            complement(1006..1383)
                     /locus_tag="VFC01_20070"
     CDS             complement(1006..1383)
                     /locus_tag="VFC01_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011015372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN81962.1"
                     /translation="MHADRRVDQPLADQVDDQLVELAVEIFTMLADATRVRIVLALKD
                     GELSVNTLAEVVGKSPAAVSQHLAKLRLARIVTTRHEGNRVFYRLANEHPLELVTDAM
                     FEAEHSLDDQPRHHNRTRQEGDR"
     gene            complement(1473..2078)
                     /locus_tag="VFC01_20075"
     CDS             complement(1473..2078)
                     /locus_tag="VFC01_20075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN81963.1"
                     /translation="MTVTAHRTRNHANMYGFARHARSYDLLSGVLARPLYRRVVADVS
                     DVGLPAGSVVLDVGTGPGRVPRLIAAAYPTVEVEGVDLSQEMIARATWTANRTRTSNL
                     RYRVGDAAALPFADNSVHLVVSTLSLHHWDDPAAGLNEIVRVLAPGGQAWIYDFRTAL
                     TATQQTTSGLDADVALESPLIGTTRLNPIARLVVRHRELPS"
BASE COUNT          433 a          763 c          808 g          421 t
ORIGIN      
        1 gtaccgatcg gatggaatgt tcggcgcggt cgaccaggga cgggtcgacg ccatccagca
       61 ggcgccgacc gatgttgccg gccgtgcccc acagcaggat gaatatcacg gtgctgatca
      121 gcaggccgac gaccggatcg gccagcggga aaccggccca caccccgatc acgccgagta
      181 ccacggccag cgaggtgaac ccgtcggtgc gggcatgcag gccgtcggcg accagcgccg
      241 cggagcggat ccggcggccc acccggatcc gatagacggc gaccagctcg ttgccggcga
      301 acccgatcag cccggcggca agcacccagc ccagattcgt caacggttgc ggctcgaaga
      361 gccggcggat cgactggtat gcggccaata ccgccgacga cgtgatcatc gtgacgatga
      421 acagcccagc caggtcctcc acgcgtccgt agccgtaggt atagcgccgc gtcgccgcgc
      481 gtcgggacag cacgaacgcc acccacaacg ggaccgcggt catcgcgtcg gagaagttgt
      541 ggattgtgtc ggccaacaac gccatcgacc cgctaacgat caccacgacg atctgcaacg
      601 tcgcggtgat cgccaacaag atcaggctga tcttcaccgc ccggataccc gcggtgctgg
      661 cctccagcgc gtcgtcgatc gagtcggccg ggtcatggct gtgcggcacg aacaagccat
      721 aaaagaaccc cctcatcccc gtcgggtgct gatgaccgtg atcatggtta tgagcgtgcc
      781 cgcgacggtg ctcgtggtca tggctttgat cgtggtcgtg gcgatcctgg acgtcgatcg
      841 agttgctgtc ctcaccgtga acatgttgag cgctgtcgcc cgtcaccgat gccgaatcgg
      901 tgtgtaaagg atcatgcggg ccggtcggat cgtccgggtg gacatgcccg gccgagcccg
      961 atgcgtgatt ttgccagtgg ccaccgacgt gcggtttcgc accgttcacc ggtccccttc
     1021 ctgccgtgtc cggttgtggt gacgcggttg gtcgtccagg gagtgctcgg cctcgaacat
     1081 ggcgtcggtg accagctcta gggggtgctc gttggctagc cggtagaaca cccggttgcc
     1141 ctcgtgccgg gtggtcacga tccgggccag ccgcagcttc gccagatgct gcgacaccgc
     1201 ggccggcgat ttgcccacga cctcggctag cgtgttcact gacagctcgc cgtccttcag
     1261 cgccaggacg atccgcaccc gggtcgcgtc cgccagcatc gtgaagatct ccaccgccag
     1321 ctccaccagc tgatcgtcga cttggtcagc cagcggctga tcaacccgtc tatctgcgtg
     1381 catacgcaga ttattgcaca gccgtttctg gaacgccggg tttcgcgcgg gcggcggcct
     1441 cggctctgga aatccgattc ggtcgagaca ggctagctgg gaagttctcg gtggcggacg
     1501 acgagacggg cgatcgggtt cagccgggtg gttccgatca gcgggctttc gagggccaca
     1561 tctgcgtcga gtccgctggt ggtctgttgc gtcgccgtga gcgcggtgcg gaagtcatag
     1621 atccaggcct gtccgccggg agcgagcacc cggacgatct cgttgaggcc ggccgccggg
     1681 tcgtcccagt ggtggaggct gagggtggag acgacgaggt ggacggagtt gtcggcgaac
     1741 ggcagcgcag cggcgtcgcc gacccggtag cgcaggttgc ttgtgcgggt tctgttggcg
     1801 gtccaggtgg cgcgcgcgat catctcttgg gacaggtcaa cgccttcgac ctcaactgtg
     1861 ggataggccg cggcgatcag cctgggcact cggcctggcc cggttccgac gtccagaacc
     1921 accgacccgg ccggaagccc gacgtcgctc acgtcggcga cgaccctgcg gtacaagggt
     1981 ctggcgagaa cgccggagag caggtcgtag ctcctagcgt gccgggcgaa gccgtacatg
     2041 ttggcgtggt tgcgggtgcg atgggcggtg acagtcatgg catctcctac agttgtatca
     2101 agcaaccttt ccgatttcag aataggcgaa acagttgcgc gatacaactg tcggttccgt
     2161 atttcccgac gacgagctgc gtcaaggatt gcaacagcta atccggctca gccgaatcct
     2221 ggaaccacac agcagcgtgg ggacagatgc ctcattgtcg gaagtcatgg cactgggaga
     2281 gctcaccgac gttgaagcaa tgtcccaaca cgaactggcg cggcggctag gtttagagaa
     2341 aagcaccgtc agccgcctcg tggcaggact cgtcgaccgc ggatgggtat cacgagcgcg
     2401 gaacccagac aaccgacgcc tctac
//
LOCUS       DASRJA010000459         4640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_264203,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000459 DASRJA010000000
VERSION     DASRJA010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264203"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFC01_20080"
     CDS             <1..273
                     /locus_tag="VFC01_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZN81964.1"
                     /translation="SPGTGVIAGGAARAVLECAGVHDILAKSLGSDNAINVVHATVAA
                     LKLLQRPEEVAARRGLPIEDVAPAGMLRARREAEALAASAAREGSA"
     gene            277..456
                     /gene="rpmD"
                     /locus_tag="VFC01_20085"
     CDS             277..456
                     /gene="rpmD"
                     /locus_tag="VFC01_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZN81965.1"
                     /translation="MAELKITQVRSTIGARWKQRESLRTLGLRKIRQSVVREDNAQTR
                     GLIKAVHHLVEVEEV"
     gene            461..904
                     /gene="rplO"
                     /locus_tag="VFC01_20090"
     CDS             461..904
                     /gene="rplO"
                     /locus_tag="VFC01_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HZN81966.1"
                     /translation="MTPLKLHDLRPAAGSKTAKTRVGRGEGSKGKTAGRGTKGTKARK
                     NVPATFEGGQMPIHMRLPKLKGFRNRFRTEYEVVNVGDINKLFPKGGDVGVDELVAAG
                     AVRKNSLVKVLGDGKLTVKVNVTAHKFSGSAREKISAAGGSATEL"
     gene            complement(967..1287)
                     /locus_tag="VFC01_20095"
     CDS             complement(967..1287)
                     /locus_tag="VFC01_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PGRS family protein"
                     /protein_id="HZN81967.1"
                     /translation="MAPLVMAGAAATAIAAAPIAAAESFAVATGASSITIPLSPGGDG
                     CYNGVCGSGDAGGGSGCTVDGTCGSGGPLGGGGCTAEGVCGHGGPWGGGGCVPGVGCF
                     NWGQ"
     gene            complement(1373..2299)
                     /locus_tag="VFC01_20100"
     CDS             complement(1373..2299)
                     /locus_tag="VFC01_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN81968.1"
                     /translation="MRFSISIPQRDTDGFDEFGFKIYLGRAEELGFEGGWTLEQTIGR
                     SPLIAPLELLAYAAACTTRLRLGVAVLITSLHDPLQLAANVTAVDRLSHGRLDVGVAP
                     GGGFRKFEAFGVEKSTFVSSFTEGLELMKAAWSDQPTVTFHGRFRDVDGLPIEPKPVQ
                     RPHPPIWFGANAPKALARAVRLGDAFLGAGSSTTASFVDAVKTVRTELDKQAKDPKGF
                     TIGKRVYLMIDDDAARARERVLAGLHRIYGDMPGIEAVPVSGTPDDVVRGLREVVDAG
                     AEMILLNPVGEHVAEDREQMERLAAEVIPQLS"
     gene            2343..2693
                     /locus_tag="VFC01_20105"
     CDS             2343..2693
                     /locus_tag="VFC01_20105"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN81969.1"
                     /translation="MADIVERYLRAIASHDWDVVDDCIVDDVVRVGPYGDRYAGRVEY
                     LAFIADLMPKLMGYAMKLGRVTYASDALAFAELSETVEVDGKPMVTPEVLVFELTGDG
                     RIARVEVFIQTPAG"
     gene            2720..4513
                     /gene="sppA"
                     /locus_tag="VFC01_20110"
     CDS             2720..4513
                     /gene="sppA"
                     /locus_tag="VFC01_20110"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HZN81970.1"
                     /translation="MFGFLPGIPGADDIRNLARRVDTARHHGVPNGCVLELDLQVLPH
                     ETGGFDPFAMIASGGRPLMLREAVAAIHRATEDPRVAGLIARVQIPAAAAGPVQELRD
                     AISAFSDVKASLAWAETYPGTLSYYLASAFREVWMQPSGTVGLVGFATNAMFLRDALD
                     KAGIEAQFIARGEYKSAANLFTQDRYTEPHREADSRLIESLHSQVRQAVAQSRHLESD
                     EVDALADKAPLLRDDAVTGRLVDRIGFRDEAYARIGELVGAQGVSPETGDADSEDAPP
                     RLYLSRYARAKASRPTPPMPSIPGRQKPTVAVVTVHGPIVSGRGGPQVLPLGRSNAGG
                     DTIAAALREAAADDSVSAIVLRVDSPGGSVTGSETIWREVNRVREGGKPVVASMGAVA
                     ASGGYYVSMGADAIVANPGTITGSIGVVTGKLIARELKDRLGVGSDSVRTNPNADAWS
                     INQPFTDEQHAHVEAEADLFYTDFVERVAKGRKMTVEAVDAIARGRVWTGADALERGL
                     VDELGGLRAAITRAKVLAGLEPDTDVRVVGYPGSSLMDLLRPKPSSQPAAASLPDAVG
                     ALVGRSVAGVLTQAERSLTGVSALWLGDYRF"
BASE COUNT          772 a         1519 c         1610 g          739 t
ORIGIN      
        1 agccccggta ccggtgtgat cgccggcggc gccgcccgtg cggtgctgga atgcgctggc
       61 gtgcacgaca ttctggccaa gtcgttgggt agcgacaacg cgatcaacgt ggtgcacgcc
      121 actgttgccg cgctgaagct gctgcagcgt ccggaagagg tcgcggcccg gcgcgggctg
      181 ccgatcgagg acgtggctcc ggcaggcatg ctgcgggcgc ggcgtgaggc ggaggcgctt
      241 gccgcgtcgg ccgcgcgtga agggtcggcg tagacgatgg cagagctgaa gatcacccag
      301 gtgcgaagca ccatcggtgc gcgctggaag caacgtgaaa gcctacggac gctggggctg
      361 cggaagatcc gccagtccgt ggtccgcgag gacaacgcac agacgcgtgg cctgatcaag
      421 gccgtgcatc acctcgtaga ggtggaggag gtctaaaaag atgacgccgc tgaagcttca
      481 cgatttaagg ccggccgccg gctcgaagac cgcgaagacc cgcgtgggac gcggtgaggg
      541 ctcgaagggc aagaccgccg gtcgcggcac gaagggcacc aaggcccgca agaacgtccc
      601 ggcgacgttc gagggtggcc agatgccgat ccacatgcgg ctgccgaagc tcaaggggtt
      661 ccgaaaccgg tttcgcaccg agtacgaggt cgtcaacgtc ggcgacatca acaagctgtt
      721 ccctaagggc ggcgacgtcg gtgtcgacga gctggtggcc gcgggtgcgg tgcgcaagaa
      781 ctcgttggtc aaggtgctcg gcgacggcaa gctgaccgtc aaggtcaatg tcaccgcgca
      841 caagttcagc ggcagtgcgc gcgaaaagat ctccgccgca ggcggttccg cgaccgagct
      901 ctagttcgct aacgaacgcg cagacccccg cacgcttagg cgtgcggggc tttttgcgtt
      961 agtgggttac tggccccagt tgaagcagcc gacaccgggc acgcagccgc cgccgcccca
     1021 tgggccgcca tggccgcaca caccctcggc ggtgcaaccg ccgccgccca gcgggccgcc
     1081 gctgccgcac gttccgtcta ccgtgcagcc gctgccgccg ccggcgtcgc ctgagccaca
     1141 gacgccgttg tagcagccgt caccgccggg gctgagggga atggtgatac tcgatgcgcc
     1201 cgtcgccacg gcgaaggact ctgctgctgc aatcggtgcc gcggcgatcg ccgtcgcggc
     1261 tgcacccgcc atgaccagcg gggcgatgaa attcaagatc gccttcatga ctccggttta
     1321 ccaccaagat cacaaactta aaacaagcta attttcagcg aaattctatg agtcagctga
     1381 gctgcggaat cacctctgca gcaagccgtt ccatctgctc gcgatcctcc gcgacgtgct
     1441 cgccgacggg attgagcagg atcatctcgg cgccggcgtc gacgacctca cgcagtccgc
     1501 gcaccacatc gtccggggtg ccggacaccg gcacagcctc gatgcccggc atatcgccgt
     1561 agatgcgatg caacccggcg agcacccgtt cacgggcgcg tgccgcgtcg tcgtcgatca
     1621 tcagatagac ccgcttgccg atggtgaagc ctttcgggtc cttggcttgc ttgtcgagct
     1681 cagtgcggac ggtcttgacc gcgtcgacga agctcgcagt cgtcgacgag cccgcgccga
     1741 ggaacgcatc gccgaggcgc accgcacggg cgagcgcctt aggggcgttg gcgccgaacc
     1801 agattggcgg atgcgggcgt tgcacgggct tgggctcgat cggcaagccg tcgacgtctc
     1861 tgaagcgccc gtggaacgtc acggtcggtt ggtcggacca tgcggccttc atcagctcga
     1921 gtccctcggt gaagctgctg acgaaggtcg acttctctac gccgaacgcc tcgaacttgc
     1981 ggaatccgcc gcccggcgcg acgccaacgt cgagccggcc gtggctgagt cggtcgacgg
     2041 cggtgacgtt ggcggccagt tgcagcggat catgcaggga ggtgatcagc accgcgacgc
     2101 cgagccgcag ccgggtggta cacgcggcgg cataggccag cagttccagc ggggcgatca
     2161 acggtgagcg tccgatggtc tgctctagcg tccagccgcc ttcgaaaccg agttcctcgg
     2221 cgcggcccag atagatcttg aagccgaatt cgtcgaagcc gtcggtgtcg cgttggggaa
     2281 ttgagatcga gaaccgcaca catccaccgt aggcgcgccc gacgccgtga tgtgatgagc
     2341 cgatggccga catcgtcgag cgctaccttc gtgccatcgc gtcgcacgac tgggacgtgg
     2401 tcgacgactg catagtcgac gatgtcgtcc gcgtcgggcc ctatggggac cgctacgccg
     2461 gtcgcgtcga atatctcgcc ttcatcgccg atctgatgcc gaagctgatg ggctatgcga
     2521 tgaagctcgg cagggtgacc tatgcgagtg acgcgctggc gttcgccgag ctcagcgaga
     2581 ccgtggaggt cgacggcaag cccatggtca cccccgaagt gctcgttttc gagctcaccg
     2641 gtgatgggcg catcgcgcgg gtcgaggtct tcatccagac gccggcgggc tagcacccgg
     2701 gcgtcggtac gctcgactca tgtttggatt cctgcccggc atccccggcg ccgacgacat
     2761 tcgcaacctg gcacggcggg tggacaccgc ccggcaccac ggagtcccga acgggtgtgt
     2821 gctcgagctg gatctgcagg tcctgccaca cgagacgggt ggcttcgatc cgttcgcgat
     2881 gatcgccagc ggaggccggc cactgatgtt gcgcgaagcc gtcgccgcca tccaccgcgc
     2941 caccgaggac ccgcgggtgg ccgggctcat tgcccgcgtt cagattcccg ccgccgcagc
     3001 gggcccggtg caggaactgc gcgacgccat cagcgccttc agtgacgtca aggcctcgct
     3061 ggcgtgggcc gagacgtatc ccggcacgct gtcgtactac ctggcatcgg cgttccgtga
     3121 ggtctggatg cagccgtcgg gcaccgtcgg acttgtcggt ttcgccacca acgcgatgtt
     3181 tctccgcgac gcgctcgaca aggcgggtat cgaggcgcag ttcatcgcgc gcggcgaata
     3241 caagtcggcg gcaaacctat tcacgcagga ccgctacacc gaaccgcacc gcgaggccga
     3301 cagccggctg atcgaaagcc tgcacagcca ggtgcggcag gcggtcgccc agtcacgtca
     3361 tctcgaatcc gacgaggttg acgcgctcgc cgacaaggcg ccactgctgc gcgacgacgc
     3421 cgtgactgga cgcctggtcg accgcatcgg cttccgcgac gaggcctacg cgcgcatcgg
     3481 tgaactcgtt ggcgcacagg gggtttcgcc ggaaaccggc gacgccgact ccgaggatgc
     3541 gccaccgcgg ctatatctgt cgcggtatgc ccgcgcgaag gcatcgcggc cgacaccgcc
     3601 catgccgtcg atcccgggtc gtcagaagcc gaccgtcgcc gtcgtcaccg tgcacggtcc
     3661 gatcgtcagc ggccgcggtg gtccgcaggt gctgccgctg gggcgctcga acgccggcgg
     3721 tgacaccatc gccgcggcgc tgcgtgaggc tgccgccgac gattcggtgt cggcgatcgt
     3781 gctgcgagtg gacagtcccg gcggatcggt caccggctcg gaaaccattt ggcgcgaagt
     3841 gaatcgagtg cgtgagggcg gtaagccggt ggtcgcgtcg atgggggcgg tcgcggcgtc
     3901 gggtggctac tacgtgtcga tgggcgccga cgcgatcgtc gccaaccccg gaacgatcac
     3961 cggctcgatc ggcgtggtga ccggcaagtt gatcgctcgc gagctcaagg accgcctcgg
     4021 cgtcgggtcg gattccgtgc gtaccaatcc gaacgccgat gcgtggtcaa tcaaccagcc
     4081 attcaccgac gagcagcacg cgcacgtcga ggccgaggcc gacctgttct acaccgactt
     4141 cgtcgagcgg gtggcgaagg gcaggaagat gaccgtcgag gccgtcgacg caatcgcgcg
     4201 tggccgggtg tggacgggtg ccgatgcatt ggagcgcggg ctggtcgacg agctcggcgg
     4261 tttgcgtgcg gcgatcaccc gggccaaggt gctggccggt ctggagcccg acaccgacgt
     4321 ccgcgtggtg ggctaccccg gctcgtcgtt gatggatctc ctgcggccca agccgtcgtc
     4381 gcaacccgca gccgcatcgc tgccggacgc agtgggcgcg cttgtcggcc gctcggtggc
     4441 cggggtgctg acccaggccg agcggtcgtt gaccggcgtc agcgccctct ggctcggcga
     4501 ctaccgcttc tgacgtcaca ttgcggcggt gatctcccgc gcgagcgcga tcaccttcgc
     4561 ggccagcggg gagtggctcc gtccggtctg ccacagcagg tggatctcac gaggggggag
     4621 cgccggagcc agctcgtgca
//
LOCUS       DASRJA010000460         4077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_288577,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000460 DASRJA010000000
VERSION     DASRJA010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288577"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VFC01_20115"
     CDS             <1..757
                     /locus_tag="VFC01_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HZN81971.1"
                     /translation="RIKLPHPDAAKFDAALASHRDALIAEWKRDHGNGDRACDHAPPL
                     PNTSDAFMRLVEAGWDAEAARRPHGHHTTVVVHVDVKQRAAALHLGPLLSEAERRYLS
                     CDATCEVWFERDGQVIGAGRATRLINRRLRRALEHRHRTCAVPGCGATRGLHAHHIRH
                     WEDGGPTELVNLVLVCPYHHRLHHRGVITITGPADDLIVTDSCGQRLSAGSLARPPNL
                     PPPAVAPCRGPTGERADWWWYEPFQPQPPPSIN"
     gene            1102..1555
                     /locus_tag="VFC01_20120"
                     /pseudo
     CDS             1102..1555
                     /locus_tag="VFC01_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244504.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
     gene            complement(1699..2280)
                     /locus_tag="VFC01_20125"
     CDS             complement(1699..2280)
                     /locus_tag="VFC01_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81972.1"
                     /translation="MGRTHGTPCSAGNFGLAELGVCALSKTYPEQLAIGELISRPSFR
                     APTLNPITPPGVLPTQAGGARSCAAVTYELTGSYAAAVIAATIGTSAGNYAAAYFTAV
                     RWSYRGQAGRCGCSSPTVLPPAIAVEFGPAEAIDSVEKTEFAGISTVPPGCYAIARDG
                     DVRIYPYWDWEIPTAEQMRADTRSDAEIVDEFR"
     gene            2348..2545
                     /locus_tag="VFC01_20130"
                     /pseudo
     CDS             2348..2545
                     /locus_tag="VFC01_20130"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2942..4018
                     /locus_tag="VFC01_20135"
     CDS             2942..4018
                     /locus_tag="VFC01_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HZN81973.1"
                     /translation="MVGALLLAVAVAGGFAVAMHKTLTLSVDGSPMTVSTMKSRVIDV
                     VRENGFAVDNHDELYPAADQPVHQSDTIVLKRGRPLQVSVDGGQSRQAWTTALTVDEA
                     LKQLSIRDAAPAAASRFSRVPLAGMVLSLVSAKKVHIVDGGVASDRRLAAPNVRLLLA
                     AAGAPLKQGDKVFPPASTSVTEGMKIVVTRIRTLTVTARITLLPRAYHVQDPTMNMSR
                     HVVENLGTTGTQVVTFAVSTVNGVETGRSPVKNEIVAPARPSVLRVGAKPATQVPPVR
                     NGATWDALSACESGGNWAINTGNGFYGGVQFNQSTWERHGGLRYAPRADLATREEQIA
                     IAEVTRTVQGWGAWPVCSVRAGVR"
BASE COUNT          680 a         1334 c         1399 g          664 t
ORIGIN      
        1 gcggatcaaa cttccgcacc ccgacgcggc gaagttcgac gcggccttgg cgtctcatcg
       61 tgatgcgctg atcgccgagt ggaagcgcga tcacggcaat ggcgaccgcg cctgcgatca
      121 cgcaccgccg ttgccgaaca cctccgacgc gtttatgcgc ctggtcgagg ccggatggga
      181 cgccgaggcg gcccgccgcc cgcacgggca tcacaccacc gtggtcgtgc acgtcgacgt
      241 caagcagcgc gccgcggcgc tgcatctggg tccgctgctg tccgaggccg aacgccgata
      301 cctgagctgt gatgccacct gtgaagtctg gttcgagcgt gacgggcagg tcatcggcgc
      361 cggccgagcg acccggctga tcaaccggcg gcttcgccgc gcgctggagc accgccatcg
      421 gacgtgtgcg gttccgggtt gtggtgccac ccgcggtttg catgcacacc atatccggca
      481 ctgggaggac ggcggtccca ccgagttggt caacctggtg ctggtatgcc cgtatcacca
      541 ccggctgcac catcgaggcg ttatcaccat caccggaccc gctgacgatc tcatcgtcac
      601 cgacagctgc ggccaacggc tcagtgcagg gtcgctcgcg cgtccgccga accttccccc
      661 gcccgcggtg gcgccctgcc gcggacccac cggtgagcgc gccgactggt ggtggtatga
      721 acccttccag ccgcagccac caccgtcaat caactaggtt ggttaccggg ccggtccgta
      781 ccagcaccgt atcgaagctc gaacaggttg gggcatagag aatttcgttc gcgatacgcg
      841 cagctaccgc atcgtgaaaa ttcgaggctg acaacccgac acgcgccgcg gctgacgcgc
      901 tatccagggc cctcatcgag atccgcgctg acgtgcgcag caatttgagc caagtcgggt
      961 gatatccgtg tgcaccgact gagcatgact gcacggcgcg acgagaacgc ccccgagcat
     1021 ttcgcggtgg cccgcgccgt cgatccgagt aagcacaagg agtttcgccg cggatcgagc
     1081 agctggagca ggagggcgcg cgtcgtggcg gccgacgcgc acagtgccgc aggcgacgat
     1141 tgggatcacg tcgtcgagga gtccgagctg ttgtccgcgg acggttccga cttcggccgt
     1201 gagcagttcc tcagcggcgc ggccacaccg gtctttcacc tcggcggcgt tgaacttcgg
     1261 cgtcaatcag ctcctcgatg tgctcgtcga actcgcgccg ccgcccagcg gaacactcga
     1321 tgtccacggg gcccgacgcg ccgccgaatc accgtttagc gccttcgttt tcaaggtgca
     1381 ggccggtatg gactccgccc accgcgatcg catcgcctac gcgcgggtgt gctcgggcac
     1441 attcgaacgc ggggacgtcc tcacccacgc cgccaccggc aaaccgttcg tcaccaagta
     1501 cgcgcagtcg gtgttcggtc agcaacgcgc caccctggac accgcgtggc cggggatgtg
     1561 atcgggctgg ccaacgtcgc tctgattcaa gcgaagcatt gcgcagcaga aagcgatcag
     1621 gactcccacg cgctcaacat gatctgcact tgctcgcgga cctccgtggc cgcggcgcgg
     1681 agcttcacga taagaggatc atctaaattc gtcgacgatc tcggcgtcgc ttcgcgtgtc
     1741 cgcccgcatc tgctcggcag tcgggatctc ccaatcccaa tacggataga tgcgaacatc
     1801 gccatctctg gcgatcgcat agcaacccgg cggcacagta ctgatcccgg cgaactcggt
     1861 cttctcgacg ctatcgatcg cctcggccgg cccgaactcg acggcgatgg cgggcggcaa
     1921 gacggttggc gacgagcacc cgcatcggcc agcttgcccg cggtacgacc agcgcaccgc
     1981 ggtgaagtac gcggccgcgt aattacctgc cgatgtgccg atggtcgccg cgatcacggc
     2041 tgcggcatac gagcccgtca gctcataggt gaccgcggcg cagctcctgg cgccgccagc
     2101 ctgagtcggc aacacgccgg gcggggtgat cgggttgagg gtcggagcgc ggaatgatgg
     2161 gcggctgatg agctcgccta tggcaagttg ttcagggtag gtcttggaga gcgcgcagac
     2221 gcccagctcg gcgaggccga agttgcccgc cgaacaaggc gttccgtgtg ttcggcccaa
     2281 tgaccgcttg ccggcgaatc acttcgctcg gctcgttgcc gagttggtcg acgagcattt
     2341 aaatcttgtg cgcattcggg ccgcggatga cgaggcgcgc agtgggccgc cctacgatcc
     2401 gcggctgatg gcgatcctgt tgtacgccta caccaccggg gtgtgctcgt cgcggatgat
     2461 cgaacgcaaa tgcgtcgacg atgccgcctt tcggtggctg accgccggcg cgtccccggg
     2521 ctatcgagcg atcgccgatt tccgcaagca gcacctgtct gcgctcggat acctgttcgt
     2581 gcaggcgttg gcattgcgcc gggcgggtgt ggtctgcctg ggccgggtcc ctgtcgacag
     2641 gacgagggtc gcgaaggaca gggctctcgt ggaggaggtc tcggccctcc tgaccggcgc
     2701 cgaacgggtc gatgaagccg aggacgcggc gttcgggaca aatcgtcgtg tcggccagca
     2761 gccgcagcga ctgcggcgac gagatattgg gctggccagg atcttcgaga ccaaggcgtc
     2821 cctggacgcc aagatcgggc cgggccggga gtggggcgcc cgggccgatg tccgcggcga
     2881 cagggatgag gccagtggca cggcggggca cactaccgcg agaccgaagc ggcggcgggc
     2941 ggtggtcggc gcgctgttgc tggcggtggc ggtcgcgggc gggttcgccg tcgcgatgca
     3001 caagacgctc acgctctccg tcgacggctc accgatgacg gtctcgacga tgaagtcgcg
     3061 ggtgatcgac gtggtgcggg aaaacggctt cgccgtcgac aaccacgacg agctgtatcc
     3121 ggccgccgat cagccggtcc atcagtccga caccatcgtg ctgaaacgcg gccggccgtt
     3181 gcaggtgtcg gtggacggcg ggcagagcag gcaggcgtgg acgacggcct tgacggtcga
     3241 cgaggccctc aagcagctgt cgatacgcga cgccgccccc gccgcggcat ctcgtttcag
     3301 ccgagtgcca ttggccggca tggtgttgtc gctggtcagc gcgaagaagg tgcatatcgt
     3361 cgacggcggt gtggccagcg accggcggct ggcggcgccg aatgtgaggc tcttgctagc
     3421 ggcggccggt gcgccgctca agcaaggcga caaagtgttc ccgccggcgt cgacgtcggt
     3481 caccgagggc atgaagatcg tggtgacccg tatccggacg ttgaccgtca ccgcgcggat
     3541 cacgctgctg ccccgggcgt accacgttca ggatccgacg atgaacatga gccgccacgt
     3601 tgtggagaac ctgggtacca ctggaaccca ggtcgtcacc ttcgcggtgt cgaccgtcaa
     3661 cggtgtcgaa accgggcggt cgccggtgaa gaacgagatc gtggcacccg cccggccctc
     3721 ggtgctgcgg gtcggtgcca agccggccac ccaggtgccg ccggtgcgca acggcgcgac
     3781 atgggacgcg ctgagcgcct gcgagtccgg cggcaattgg gcgatcaaca ccggcaacgg
     3841 tttctatggg ggcgtgcagt tcaaccagag cacgtgggag cggcacggtg gactgcgtta
     3901 cgcgccgcga gcagaccttg ccactcggga agagcagatc gccatcgccg aggtgactcg
     3961 gacagttcag ggctggggcg cctggccggt atgcagcgtc agagcggggg tgcgctgacc
     4021 aacacaccct cgccgtccgg gccgggccag tatcctgcgc tactcacttc gtgggag
//
LOCUS       DASRJA010000461        17945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2388792,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000461 DASRJA010000000
VERSION     DASRJA010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17945
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2388792"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="VFC01_20140"
     CDS             complement(<1..160)
                     /locus_tag="VFC01_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZN81974.1"
                     /translation="MAPKKKVIGLIKLQIQAGQANPAPPVGPALGQHGVNIMEFCKAY
                     NAATENQRG"
     gene            complement(242..1021)
                     /gene="nusG"
                     /locus_tag="VFC01_20145"
     CDS             complement(242..1021)
                     /gene="nusG"
                     /locus_tag="VFC01_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZN81975.1"
                     /translation="MTSFEGESPSAESVDVIDETNVDSVTEVDPVTEDADAAPAEVAA
                     EPAPEAEEEDPAVALKKDLRTRPGDWYVIHSYAGYENKVKANLETRVQNLDVGDYIFQ
                     VEVPTEEVTEIKNGQRKQVNRKVLPGYILVRMELNDESWGAVRNTPGVTGFVGATSRP
                     SPLSLDDVVKFLLPPAAAKKPGKAASTAAAAAETGGIERPVIEVDFEVGESVTVMDGP
                     FATLPASISEVNAEQQKLKVLVSIFGRETPVELTFNQVAKI"
     gene            complement(1048..1500)
                     /gene="secE"
                     /locus_tag="VFC01_20150"
     CDS             complement(1048..1500)
                     /gene="secE"
                     /locus_tag="VFC01_20150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZN81976.1"
                     /translation="MSDERDGAGSPGDGSVTDSVTDSTGDNRGQTAVVTRPARPTGKR
                     SRRGAVEDDSEAVDLETGTKAAPTKNGAGTATKTAKKAADRPSRNPIAFVWNYLLQVV
                     AELRKVIWPNRKQMINYTAVVLVFLAFMVALIGLVDMGLVKLVTLIFG"
     gene            complement(1569..1641)
                     /locus_tag="VFC01_20155"
     tRNA            complement(1569..1641)
                     /locus_tag="VFC01_20155"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1606..1608),aa:Trp,seq:cca)
     gene            complement(1787..2323)
                     /gene="hadC"
                     /locus_tag="VFC01_20160"
     CDS             complement(1787..2323)
                     /gene="hadC"
                     /locus_tag="VFC01_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(3R)-hydroxyacyl-ACP dehydratase subunit HadC"
                     /protein_id="HZN81977.1"
                     /translation="MALKTDIRGMVWRYPDTFVVGREQVRQYAKAVKSLDPATHDEDA
                     AAALGHDALIAPLTFASIFAVMIQTHFFQHVDVGMETMQIVQVDQKFKFHRPIKVGDE
                     LTGTMHVESVDERFGADIVTTRNVCATPDGTIVMEAFTTLMGHEGENSISAKWDPETG
                     QVIRTAVSENGQNGQTAD"
     gene            complement(2327..2755)
                     /gene="hadB"
                     /locus_tag="VFC01_20165"
     CDS             complement(2327..2755)
                     /gene="hadB"
                     /locus_tag="VFC01_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(3R)-hydroxyacyl-ACP dehydratase subunit HadB"
                     /protein_id="HZN81978.1"
                     /translation="MALREFDSVKVGDQLPEKVIPLTRGDLVNYAGVSGDLNPIHWDD
                     DIAKQVGLDTAIAHGMLTMGLGGGYVTSWIGDPAAVTEYNVRFTAVVPVPNDGVGAEI
                     VFNGRVKSVEPETKSVTIALTATAGGKKIFGRAVATAKLA"
     gene            complement(2742..3221)
                     /gene="hadA"
                     /locus_tag="VFC01_20170"
     CDS             complement(2742..3221)
                     /gene="hadA"
                     /locus_tag="VFC01_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(3R)-hydroxyacyl-ACP dehydratase subunit HadA"
                     /protein_id="HZN81979.1"
                     /translation="MSLSEQIVGMHYRYPDHYVVGREKLREYATAVKNDDAAFYDETA
                     AAELGHEALPAPLTFISVFGYQAQSAFFASANIGITDMQIVQVDQVLKFLKPIHAGDK
                     LYCDVYVDSVRQSHGADIIVTKNIVTNDKGDVVQETYTTLAGRSGEDGEEGFSDGTA"
     gene            complement(3276..3443)
                     /gene="rpmG"
                     /locus_tag="VFC01_20175"
     CDS             complement(3276..3443)
                     /gene="rpmG"
                     /locus_tag="VFC01_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZN81980.1"
                     /translation="MASSTDVRPKITLACEVCKHRNYITKKNRRNDPDRLELKKFCPN
                     CGKHQAHKESR"
     gene            complement(3481..3554)
                     /locus_tag="VFC01_20180"
     tRNA            complement(3481..3554)
                     /locus_tag="VFC01_20180"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3518..3520),aa:Met,seq:cat)
     gene            complement(3588..3660)
                     /locus_tag="VFC01_20185"
     tRNA            complement(3588..3660)
                     /locus_tag="VFC01_20185"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3625..3627),aa:Thr,seq:ggt)
     gene            3858..4730
                     /locus_tag="VFC01_20190"
     CDS             3858..4730
                     /locus_tag="VFC01_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZN81981.1"
                     /translation="MTEPGTPRVAVYLDFDNIVISRYDQIHGRNSFQRDRTAGFHKTP
                     GRLTTATVDVGAIIDFASSFGTLVLTKAYADWSADVNADYRGQLVGRAVDLVQLFPAA
                     AYAKNGADIRLAVDAVEDMFRLPDLTHVVIVAGDSDYIALAQRCKRLGRYVVGIGITG
                     SISRSLTAACDEFVTYDALPGVPAVAPAPAKKTTRRTKAQEPDDEAEQPDPQAAATGL
                     LERALRIGHEKDDADWLHNSAVKAQMKRMDPSFSEKSLGFRSFSDFLRSRTDVVDLDE
                     SSTTRLVRLKAADT"
     gene            4740..5450
                     /locus_tag="VFC01_20195"
     CDS             4740..5450
                     /locus_tag="VFC01_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN81982.1"
                     /translation="MTDRLYFRQLLSGRDFAAGDMIAAQMRNFAYLIGDRETGDAVVV
                     DPAYAANDLVDAMEADDMHLSGVLVTHHHPDHVGGSMMGFELKGLAELLERTSVPVHV
                     NSLEADWVSRITGIARSELTAHEHGDVVNVGGIDIELLHTPGHTPGSQCFLVDGRLVA
                     GDTLFLEGCGRTDFPGGDVDAIYHSLQALAQLPGDPTVFPGHWYSAEPSASLSEVKRT
                     NYVYRASNLDQWRMLMGA"
     gene            5512..6543
                     /locus_tag="VFC01_20200"
     CDS             5512..6543
                     /locus_tag="VFC01_20200"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HZN81983.1"
                     /translation="MTTPPTPAGWYPDPDGSGGQRYWDGSGWTEHRAPAAPEPAEPAA
                     PAEPPASEQPTAIVPRRPAEEHVGSHRAPDATPEPEPTPEPEPTPEPEPTPAPEPEPG
                     PTAVINLGSVPSAQPTTPVTPPTPPAEPYWATTPPSGEPSDDRRKLIIWFGAACAALL
                     AILVLVVIYGVFINKNDTVHISTGSGTTSKSEAPTTTSGNGWGTETETASESPTTASG
                     SGSQASDGGLTFGVTGVETAPSVQYQDAPVEKTAQGEFIIVHLTVLNSGDQPVTYLGT
                     LQKLKAGGTTYSIDDEATFYLNGGLAELNPGDSADVAIAFDVPPGTTPESLEAHGDPI
                     SAGVNVPLS"
     gene            6564..7214
                     /locus_tag="VFC01_20205"
     CDS             6564..7214
                     /locus_tag="VFC01_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81984.1"
                     /translation="MADTLYADVSEWQVGVNDSYPYPVLCIRSNDGTHRDRRWVNNYG
                     WCLRNADSGRLTFFIVYFVWRPNWQQTVDTFKDLVGTPHPKMAVMLDVESWGGQIRGD
                     RSAGINAAYEAVGAFVGSTAKVIGYGNVGDLNSVWPRKPPGIRLVVAAYGHNPPYPGK
                     VAHQYTDGSGYGGGLPEGAPPFGRCDMNSADGLTAVEFARACGIAATESAPTDVAV"
     gene            7322..8329
                     /locus_tag="VFC01_20210"
     CDS             7322..8329
                     /locus_tag="VFC01_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HZN81985.1"
                     /translation="MKNIVLCFDRASDHPGSGDATNAEALLRLLDESDDQITWYHSGM
                     RPDPQDGGALARLRWREAIADDARATIAEAYEFLVDSWEPGDRIYMFGVGRGAYCAYS
                     VTRLLGTVGLLPDLMDYVLAAYAVPRTRRTPQDWRRVTLLAERLAGHREIGVPVAFLG
                     LWDMAQVPGCRATQEPLSNVVAGRHAVAIDGRRGSRNLVASPDCVEEVWFRGAHCDVA
                     GGPGACWPQADIALDWMLYGAVQAGVTMRDASQCEVPTPTEYGALAGSARTVSLRRLP
                     ASPLVHASVDMYLRAHPEYWRRLPAHVVWADTDWLARSERLVPAESPNVPVEHGVLAA
                     S"
     gene            complement(8333..9424)
                     /locus_tag="VFC01_20215"
     CDS             complement(8333..9424)
                     /locus_tag="VFC01_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HZN81986.1"
                     /translation="MTNPQSTERNLVLCLDGTNNEPETGATNVARIYDLAEKNANQLV
                     YYDPGVGTMGARGSVTRFGKAMTRVAGLVAGFGIKDNLEEAYTWLSRNYQKGDRIYVF
                     GFSRGAYTARALTGMLRTVGLLRPGSENLVPYAVKLYTKAGPGAEVGPEAKAAEKKFW
                     DQRDEFVRVFGNPDFPTMFNRAVPQVHFLGVWDTVKTVGWLNFKARFETARWPFTRKI
                     DNVGTARHAMAIDERRRLYAPYRFDQATVAGSAGHHQEMWFAGVHSDVGGQFPDDHRL
                     SDIALSWMVHAAERAEFRVIKQKYRRMLGVRFDEELPADHAMGRIHTNENIWRLLGFR
                     RREIPNGDRIHPTVQYRILHSDYRPDLKR"
     gene            9488..9946
                     /locus_tag="VFC01_20220"
     CDS             9488..9946
                     /locus_tag="VFC01_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HZN81987.1"
                     /translation="MSTPRPTLQTDPEWPHEASDAVDRFVAGLQTGIDSADAELYNAD
                     FSDDVMWGSPYGATVIGYDALHSIHRGMFDHGVAVAGASSRYETVRIMAPTLDVAIAQ
                     VRRVALDADGEPIPIDDPSVFSEMAMYVLVRRDGKWWLAAGQNTPVRPKP"
     gene            10116..10937
                     /locus_tag="VFC01_20225"
     CDS             10116..10937
                     /locus_tag="VFC01_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81988.1"
                     /translation="MDWFQDHIAGISAGAWTALAAWLAVVAGIAALVYAWRQYQRAKE
                     QTAELMQPNVAMFMEPSPGDWHLIELVVKNFGRTPAYGIRFEFANPPTVGKYESAYED
                     RYVDITPLNLPAEIPYLAPSQEWRIVWDSALDRRELGEAIASRFDGAVTYYDQPKGGA
                     RGKQTRSQYRSTAVLDWATLHPVERLELLTTHDLARQERQKLELLRNLLTYFHYAASE
                     SREEVLRTEINRVRAAADELRERYRSQYQAPVNNNFDFDEAHTTVINGRHRRDLI"
     gene            complement(10934..11923)
                     /locus_tag="VFC01_20230"
     CDS             complement(10934..11923)
                     /locus_tag="VFC01_20230"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZN81989.1"
                     /translation="MNPVERAVRSVDCWQQRHPIAAFPVAVVKKFGDDEAGNLVALLT
                     YYGFLATFPLLLALTAVLGVALRKYPELHAKLVNSAFAEFPIVGAEIHEQLGVEKFSG
                     TAIALTAGIVVAILGGRGFAHALQNTLNTVWAVPKTDRPGLAPRYLRTITVLLLLGLI
                     VMVTGAASTAAGVATSVGLGGMPARFVSFAVGTILGFGFFLALFRVAAAAQVPTRSLL
                     LSAAVSAVGWQLLLILAGYVVAHQLRHAQAVAGLFGVVLGLLAWLALQATVIVYAIET
                     DVVRVKHLWPRSIIQPPLTKADEAYYTDALRAEAQRPEQQIDIAYEKQPADPC"
     gene            complement(11987..12127)
                     /locus_tag="VFC01_20235"
     CDS             complement(11987..12127)
                     /locus_tag="VFC01_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81990.1"
                     /translation="MRSMRKRSAEHLESEAGKTTKRRKQPRNSGVPKLSLWECGIGSV
                     WK"
     gene            12272..12973
                     /locus_tag="VFC01_20240"
     CDS             12272..12973
                     /locus_tag="VFC01_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZN81991.1"
                     /translation="MADTVKYELNDAVATITLDDGKVNVLGPAMQSAINEVLDKAEKD
                     SAKAVVIAGNQRVFSGGFDLAVFQSGDAKAALGMLAGGFELSLRCLTFPVPVIMAATG
                     PAIAMGSFLLLSGDHRVGQPKSRCQAIEVAIGMTIPISAVEIMRARLTPAAFHRGASM
                     AATFAGDEALAGGWLDELVEADRVLARAQEVAAEAAATLHAGAHLATKLKARDSALKA
                     IQAGIDGLATEFTLG"
     gene            12973..13557
                     /locus_tag="VFC01_20245"
     CDS             12973..13557
                     /locus_tag="VFC01_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN81992.1"
                     /translation="MPRAKQRTPELRDRLLEVAIETLSEEGIARFTTRRVAERAGTSV
                     PAVYELFDDKAGLVRAMFFEGFRLLGGELAKVPVTTDPVADLERLVPVFRLFCRAYPR
                     LAQVMFSRPFADFEPGPEELAAGASVREVFVGRIERCIDAGLLSGDPNDMAHVLLALA
                     QGLAVQEAGRWLGSGPLIERRWKLGVTSLLAGLR"
     gene            13565..14998
                     /locus_tag="VFC01_20250"
     CDS             13565..14998
                     /locus_tag="VFC01_20250"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN81993.1"
                     /translation="MAGRIGSMFGPYELQALLGVGGMGEVYRAYDTSKDRTVALKLLR
                     AEMSADAGFQERFRRESRIAARLQEPHVIPVHDFGEINGVLYIDMRLVEGNNVHNVLQ
                     QNGPLDPARAVSIVGQVAAALDAAHASGLVHRDVKPENVLLTHTDFAYLVDFGIAHLG
                     GDTGLTSAGAAIGSCAYMAPERFTGGRVGPAADVYSLACLLYECLTGSAPFPTGELSQ
                     LMGAHVMSPPPRPSATRPIVGTAFDGIVARGMAKQPEERFSSAGDLARAANAAVMAAQ
                     VPTAFNRLTAPPPPPQSPPVRRGFGRVQWLLAGAAFGTLAAAVGVVLWLVLGQDKTEH
                     DVAPPTTTSAAPPATTSAEPPTTSPPTSTSPEAPVTLPGTDAQGFIDYPGARCDAGTS
                     PAALARTTQSVFVVCRSGPGDFYYRGVRLSDGAGIELANAVRSSGGFDVTNPTDGTRY
                     QIRPDGLTIITPNGQTFTEPMVAYGTS"
     gene            complement(15015..15428)
                     /locus_tag="VFC01_20255"
     CDS             complement(15015..15428)
                     /locus_tag="VFC01_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81994.1"
                     /translation="MSRVSRFATAVVVSGGVTVAGLGLAAGAAQADPAWGPSYNGGNC
                     PTAYTCTHWCPGDPLIPGSQVISWDWNVCHDWYWNSEGVVDVAANIIYPWRGTPHDAP
                     PPPVVAPYTPPPPQPLPPDCPWHSPFLAPSRCGGL"
     gene            15564..16547
                     /locus_tag="VFC01_20260"
     CDS             15564..16547
                     /locus_tag="VFC01_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HZN81995.1"
                     /translation="MTHVRGRVWRAGKPQDDFDFPSISDYLAEDDALVWCDIYDPDHA
                     ILKDLAEELSLNTWAVEDAIAESERTKAVVYRTHTFFTVYGVTTKETTDETESMLDIH
                     RISGFVLPRGLITVRLSPHFDIEAVSQRFDELGGQEHGVGTLVHGLLDVVVDGHFEAV
                     QQLDDGIESIEDDLFENNPRSGLQRKTFRLRKDLVELRRVVLPMREVVNSIQHRRLDA
                     KTAPELDPLYSDLYDHVLRVSEWTESLRDMVTTVFETNLSLQDARLNTVMKKLTGWAA
                     IIAVPTAITGFYGQNVEYPGINTFWGFLASTSIIVLIVLALYWIFRRRNWL"
     gene            16778..17110
                     /locus_tag="VFC01_20265"
     CDS             16778..17110
                     /locus_tag="VFC01_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN81996.1"
                     /translation="MLTTTTRAELKLSTEWPNSTVVRIRASGDIDASNSAEMLDYVLR
                     RGANCRSLVLDLSDVTFFGTAGFSALQTIDVRSSRASVSWMLVAGPAVSRVLSICDPQ
                     RELPRAVA"
     gene            complement(17115..>17945)
                     /locus_tag="VFC01_20270"
     CDS             complement(17115..>17945)
                     /locus_tag="VFC01_20270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HZN81997.1"
                     /translation="CGAFPVPLSIPGAEAALPLRSFADAVILRMAARSADSAVVIGGG
                     LIGCEAAACLASRGVPTTMVAAEPVPLQRRFGDEAGERVAKILSDNGVRFLGSTTVTA
                     IYDTHVHLDTGAALDGDVVVSASGVRPDPHLAEAAGLDTYDGRIVVNERMHTSALNVY
                     AAGDVALAYNVTAGRRIRAEHWRDAAQQGLIAGLSAAGQPAVWDTVPGFSCTIGESTL
                     KYRGWGLGYEHSRLVDHHNGFTVYYESCGDLVGVLTLNADDDYRLADRLVRSHAPMMI
                     "
BASE COUNT         3020 a         5742 c         5957 g         3226 t
ORIGIN      
        1 tgccgcgctg gttctccgtc gcggcgttgt acgccttgca gaactccatg atgttgacgc
       61 cgtgctggcc gagcgccgga cccaccggcg gggcggggtt ggcctgcccg gcctgaatct
      121 gcagcttgat cagcccgatg actttcttct tcggggccat gggtgttggt gttcctctct
      181 cgtttcagtg atttcggcgc gcttaaggtc gctgagcgac cgtttccgcg ccgaaatcgc
      241 tctagatctt ggcgacctgg ttgaaggtca gttcgacagg tgtttcgcgg ccgaagatgg
      301 acaccagcac cttgagcttc tgttgttcgg cgttgacctc gctgatcgac gcgggcaacg
      361 tcgcaaacgg tccgtccatg acggtgaccg actcgccgac ctcgaaatca acctcgatga
      421 cgggccgttc aattccaccg gtctcggcgg cggcagcggc ggtcgatgcg gccttgccgg
      481 gcttcttggc cgcggcgggg ggcagcagga acttgaccac atcgtccagc gacagcggcg
      541 acggccgcga cgtcgcgccg acgaagccgg tcacgccggg cgtgttgcgc accgcgcccc
      601 acgactcgtc gttgagctcc atgcggacca ggatgtaacc cggcagcacc ttgcggttga
      661 cctgcttgcg ctggccgttc ttgatctcgg tgacctcttc ggtgggcacc tcgacctgga
      721 agatgtagtc gccgacgtcg aggttctgca cgcgggtctc gaggttggcc ttcaccttgt
      781 tctcgtagcc ggcgtaggag tggatgacgt accagtcgcc gggccgggtg cgcaggtcct
      841 tcttcagcgc gacggccgga tcctcttcct cggcttccgg agccggttcg gcggcaacct
      901 cggcgggtgc ggcgtcggcg tcctcggtca cggggtcaac ctctgtcacc gagtcgacgt
      961 tggtctcatc gatgacgtcg acgctctcgg ccgaaggcga ttcgccctcg aaggaagtca
     1021 cttgtcagtc ctctctcatt ccgctcgtca gccgaatatc agcgtcacga gcttgaccag
     1081 gcccatatcg accaggccga tcaacgccac catgaacgcc aggaacacca gcacgaccgc
     1141 ggtgtagttg atcatctgct tgcggttggg ccagatcacc ttgcgcagtt cggcgacgac
     1201 ctgcagcagg tagttccaga cgaaggcgat cgggttgcgc gacggccggt cggcggcctt
     1261 cttggccgtc ttcgtcgcgg tgcccgcgcc gttcttggtc ggggccgcct tggtaccggt
     1321 ctcaaggtcg acagcctcgc tgtcgtcttc caccgcaccg cgccgggacc gcttgcccgt
     1381 cgggcgcgcc ggccgggtca ccaccgcggt ctgaccgcgg ttgtcaccgg tgctgtcagt
     1441 gacgctgtcg gttacggagc cgtcgcctgg ggagccggca ccatcgcgct cgtcgctcac
     1501 cgcatgcctt tcgtctggtt tctgtgggtc accttccagt gtccacgctt gtcgagtatt
     1561 cagttgcgca ggggcgacag gacttgaacc tgcaacctgc ggttttggag accgctgctc
     1621 tgccagttga gctacgcccc tctatggatg gcactgctgg caggtccacc ctgccggggc
     1681 ttacacaatt gcgcgctccc cgatcggtcg atcagaaacc gacggacagc gcgctggact
     1741 gggaaaaccc cgaggtccga gtgtagcgcg ccacccgtgg tagttactaa tccgccgtct
     1801 gcccgttctg tccgttctcg ctgacggctg tgcggataac ctggcccgtc tccggatccc
     1861 atttcgccga gatcgagttt tcgccctcgt ggcccatcag cgtggtgaac gcctccatca
     1921 cgatggtgcc gtccggggtg gcgcacacgt tgcgggtggt gacgatatcg gcgccgaacc
     1981 gctcgtcgac cgactcgacg tgcatggtcc cggtcaactc atcgccgacc ttgatcggcc
     2041 ggtggaattt gaacttctgg tcgacctgga cgatctgcat ggtttccatg ccgacgtcga
     2101 cgtgctggaa gaagtgcgtc tggatcatca ccgcgaagat cgaggcgaac gtgagcggcg
     2161 cgatgagggc gtcgtggccc aatgcggctg ccgcgtcctc gtcgtgggtc gccggatcga
     2221 gtgacttgac cgccttggcg tactggcgga cctgttcgcg tcccacgacg aaggtgtcgg
     2281 ggtagcgcca caccattccg cggatgtcag ttttgagggc catgggtcac gcgagctttg
     2341 cggtagccac ggcgcggccg aagatcttct ttccccccgc agtggccgtc aatgcgatcg
     2401 ttacggactt cgtctcgggt tcaacggatt tcacgcgacc attgaacacg atttcggcac
     2461 ccacgccatc gttgggaacc gggaccacgg cggtgaaccg cacgttgtac tcggtgaccg
     2521 cggcgggatc accgatccag gatgtgacgt atccgccgcc caatcccatg gtgagcatgc
     2581 cgtgcgcgat cgcggtgtcc aggccgacct gcttggcgat gtcgtcgtcc cagtggatcg
     2641 ggttcaggtc gccggagacg ccggcgtagt tcaccagatc gccccgagtc agggggatga
     2701 ccttctcggg aagctggtca cccaccttca ccgaatcgaa ctcacgcagt gccatcgctg
     2761 aaaccctctt ctccgtcttc acccgatcga cccgccaggg tcgtatacgt ctcctgcacg
     2821 acgtcaccct tgtcgttggt gacgatgttc ttggtcacga tgatgtcggc gccgtgggac
     2881 tgccgaacgg agtccacata cacgtcgcag tagagcttgt cgccggcgtg aatcggcttg
     2941 aggaacttca gcacctgatc gacctggacg atctgcatat ccgtgatgcc gatgttggcg
     3001 ctggcgaaga acgctgactg cgcctggtat ccgaaaaccg agatgaacgt gaggggtgcg
     3061 ggcagcgcct cgtggccgag ttcggcagcg gcggtctcgt cgtaaaacgc cgcatcgtcg
     3121 ttcttgacgg cggtcgcgta ctcgcggagc ttctcgcgac cgacgacgta gtggtcgggg
     3181 tagcggtagt gcatcccgac gatctgttct gacagcgaca cggttgcttg aacctatcgg
     3241 tcaaccctgg gcaaacgccc tcgggttggc gcgcgctagc gcgattcctt gtgcgcctgg
     3301 tgcttgccgc agttcgggca gaacttcttc agctcaagcc ggtcggggtc gttacggcgg
     3361 ttcttcttgg tgatgtagtt gcggtgttta cacacctcgc aggccaaagt gatcttcggc
     3421 cgtacatctg tactggaggc cacgtcagct tcctctttct cacatgtctg tttcgtcttg
     3481 tagcgatggc cggactcgaa ccggcgacct aacgattatg agtcgttcgc tctaaccgac
     3541 tgagctacac cgccatgtcg ggcgccagac gagctcggcg tccatcgagc cccctaacgg
     3601 aatcgaaccg ttgacctttt ccttaccatg gaaacgctct gccgactgag ctaagggggc
     3661 ttcgtctttc cagacccgcg gaaaaccgtg ccgtgggcct taaagagggt acattctcgc
     3721 ggtttgacgc gccaaaccgc tggtcctgtc gcgctacaga ccgaccgtca caccgttgcc
     3781 gtcacgtacc tcccggcggt gtttgccgag attcgcgatc gggccgcagt aggcccgcca
     3841 ggtgatagga cggaagtgtg actgagcccg gcacgccccg cgtggcggtg tacctcgact
     3901 tcgacaacat cgtcatttcg cggtacgacc aaatccacgg ccgcaactcc tttcagcgtg
     3961 acaggacggc gggcttccac aagacgcccg gacggctgac caccgcgacc gtcgacgtcg
     4021 gcgccatcat cgacttcgcc tcatcgttcg gcaccctggt gctgacgaag gcctacgccg
     4081 actggtcggc ggacgtgaac gccgattacc gcggccagct ggtcggtcgc gcggtcgacc
     4141 tcgtgcagct cttccccgcc gcggcgtacg ccaagaacgg ggccgacatc cggctggcgg
     4201 tcgacgcggt cgaggacatg ttccggttgc ccgacctgac ccacgtggtc atcgtcgccg
     4261 gagactcgga ctacatcgcg ctcgcgcagc gctgcaagcg gctcggccgc tacgtcgtcg
     4321 gcatcggcat cacgggttcg atcagcaggt cgctcacggc cgcctgcgac gagttcgtca
     4381 cctacgatgc actgccgggt gtgccggcgg ttgcgccggc gcccgccaag aagacgacgc
     4441 ggcgcaccaa ggctcaggag cccgacgacg aagccgaaca gccggatccg caggctgctg
     4501 ccaccggcct gctggagcga gcgctgcgga tcggccatga gaaggacgac gcggattggc
     4561 tgcacaactc ggcggtcaag gcccagatga agcggatgga cccgtcgttc tccgaaaagt
     4621 cgctgggatt ccggtcattc agcgacttcc tgcgctcgcg caccgacgtg gtggacctcg
     4681 acgagagcag caccacccgc ctggtgcggc tcaaagctgc cgacacatag tctgaagcca
     4741 tgaccgaccg cctgtacttt cggcagctgc tctcggggcg cgacttcgcg gccggcgaca
     4801 tgatcgcggc gcaaatgcgc aacttcgcct acctcatcgg cgaccgcgaa accggcgacg
     4861 ccgtcgtcgt cgacccggcc tacgctgcca atgacctcgt cgacgcgatg gaggccgacg
     4921 acatgcacct gtccggcgtc ctggtcaccc accaccaccc cgatcacgtc ggcgggtcga
     4981 tgatgggctt cgaactcaag ggcctcgccg agctgctgga gcgcaccagt gtgccggtgc
     5041 acgtcaattc ccttgaggcg gactgggttt cacgcatcac cggcatcgcg cgctccgagc
     5101 tcaccgcaca cgagcacggc gatgtggtca acgtcggcgg catcgacatc gaactgctgc
     5161 acacccccgg ccatacgccg ggcagccagt gcttcctggt cgacgggcgg ctcgtcgccg
     5221 gcgacacgct gttcctcgaa ggctgcgggc gcaccgactt cccgggcggc gatgtcgacg
     5281 cgatctacca cagcctgcag gccctcgcgc agttgccggg cgaccccacc gtgtttcctg
     5341 gccattggta ttcggcggag ccgagtgcgt cgctgtcgga agtcaaacgg accaactacg
     5401 tgtatcgggc cagcaatctc gatcagtggc gcatgttgat gggcgcctga cgcgactgtt
     5461 tgcctgagca aaagtcgcgc cgcttcctgt gaactgggtt attctcccgc gatgaccaca
     5521 ccgcccacgc ctgcaggttg gtatcccgat cccgacggct caggcggcca acggtattgg
     5581 gacggatccg ggtggaccga acaccgggcc cccgccgccc ctgagccggc agagcccgcg
     5641 gcccccgccg agccccccgc cagcgagcag ccgacggcca tcgttccgcg ccgccctgcc
     5701 gaagagcacg tcggctcaca ccgggcgccc gatgcgacac ccgaaccgga gccgacgccg
     5761 gagccggagc cgacgccgga gccggagccg acgcccgcac ccgaacccga gccggggccg
     5821 actgccgtca tcaacctggg atccgtgccc agcgcgcagc cgaccacccc cgtgacaccg
     5881 ccgacacctc cggccgagcc gtactgggcc accacacctc cctccggcga gccatcggac
     5941 gaccgccgca agctcatcat ctggttcggc gcagcatgcg cggccctgct cgcgatactt
     6001 gtccttgtcg ttatctacgg ggtgttcatc aacaagaatg acaccgtcca tatttcgacg
     6061 ggttccggca cgacgtcgaa gtccgaggcc ccgacgacca cgtcgggcaa tggctggggc
     6121 accgaaaccg agaccgcaag cgaatcccca acgaccgctt caggttccgg ttcccaggcc
     6181 tccgacggcg gcctgacgtt cggggtcacc ggtgtggaaa ccgcgccgtc agtgcagtat
     6241 caggacgcgc cggtggagaa gaccgcgcag ggcgagttca tcatcgtgca cctgaccgtg
     6301 ttgaattccg gcgatcagcc cgtcacgtac ctcggcacat tgcagaagct gaaggcgggc
     6361 ggcaccacgt actcgatcga cgacgaggca acgttctatc tcaatggcgg cctggcggaa
     6421 ctgaatccgg gtgattcggc agatgtagca atcgctttcg atgtgccgcc ggggaccaca
     6481 ccggagtcgc tcgaggcgca cggcgatccg atcagcgccg gcgtcaacgt tcccttgtcg
     6541 tagtcgcctg cagactggag agcgtggcag acacgctcta tgccgatgtg tccgagtggc
     6601 aggtcggcgt caacgacagt tacccgtatc cggtgttgtg catccgatcc aatgacggca
     6661 cgcatcgaga tcgccgctgg gtcaacaact atggctggtg cctgcgcaat gccgacagtg
     6721 gccgactgac cttcttcatc gtctactttg tctggcggcc gaattggcag caaacggtcg
     6781 acacgttcaa ggatctggtc ggcacaccgc atccgaagat ggcggtgatg ctcgacgtgg
     6841 agtcgtgggg cggccagatc cgcggcgacc ggtcggcggg aatcaacgcg gcctatgagg
     6901 cggtcggcgc gttcgtcggg tcgacggcga aggtgatcgg ctacggcaat gtgggcgatc
     6961 tcaacagtgt gtggccacgc aagccgccgg gcatccgact ggtggtcgca gcctacggcc
     7021 ataacccgcc gtatcccggg aaggtcgcgc accagtacac cgacggttcg ggctacggcg
     7081 gcggtttgcc ggagggcgcg ccgccgttcg gccgctgcga tatgaactct gcggacgggc
     7141 tcacagcggt ggaattcgcc cgtgcctgcg gcatagcggc gacggaatcc gcgcccaccg
     7201 acgtggcagt gtaggaattc cgcggaattt cagaattcga gtagtgcaat acgcaggaca
     7261 cggcgctctt tgcggagcaa actaatcgga agcagcgtaa atacttccgt cgggggtcgt
     7321 cgtgaagaac atcgtgctgt gctttgaccg cgcaagtgac catcctggtt ctggtgatgc
     7381 cacgaatgcc gaggcgttac tgcgtctgct cgacgagagc gacgaccaga tcacgtggta
     7441 ccactccggc atgcggccgg acccgcaaga cggcggcgcg ctggcccggc tgcggtggcg
     7501 cgaggccatc gccgacgacg cccgcgcaac gatcgccgag gcatacgaat tccttgtcga
     7561 ctcgtgggag cccggcgatc ggatctacat gttcggcgtt ggccgtggcg cctactgcgc
     7621 ctactcggtg acccggctgc tcggcaccgt cggcctgttg cccgacctga tggactatgt
     7681 gctcgccgcc tatgccgtgc cgcggacgcg tcgcaccccg caggactggc ggcgggtcac
     7741 gctgctggcg gaacgactgg ccgggcatcg tgaaatcggg gttcctgtag cgtttttggg
     7801 actgtgggac atggcgcagg tgccaggttg tcgggcgacg caggaaccgc tgagcaacgt
     7861 cgtcgcgggc cgacacgcgg tggcgataga cggccgccgc ggttcgagaa accttgtggc
     7921 atcgcccgac tgcgtcgagg aagtgtggtt ccgcggggcg cattgcgatg tcgcgggcgg
     7981 gccaggggca tgctggccgc aagccgatat cgcgctggac tggatgctct acggcgccgt
     8041 gcaggccggt gtcaccatgc gggacgccag tcagtgcgag gtgccgacgc caaccgaata
     8101 cggcgccctg gccggcagcg cgcgcactgt ttcgctgcga cggctgcctg ccagcccgtt
     8161 ggtacatgcc agcgtcgaca tgtatctgcg ggcccatccg gagtactggc gacgactgcc
     8221 cgcccacgtg gtgtgggccg acaccgactg gctcgcccgc agcgagcggc tcgtaccagc
     8281 cgagtcgccg aatgtgcctg tcgagcacgg ggtgctcgcg gcgtcctgac tgctaccgct
     8341 tgagatccgg gcgatagtcg ctgtgcaaaa tgcggtattg cacggtcgga tgaatgcggt
     8401 cgccgttggg gatctcgcgt ctgcggaatc cgagcagccg ccagatgttc tcgttggtgt
     8461 ggatccgccc cattgcgtga tcggcgggga gctcttcatc gaaccggacg ccgagcatcc
     8521 gccggtactt ttgtttgatg acacggaatt cggcgcgctc ggccgcgtgc accatccacg
     8581 acaatgcgat gtccgacagc cggtggtcgt cggggaactg gccgccgaca tcactgtgca
     8641 caccggcgaa ccacatctcc tgatggtggc ctgccgaccc ggcgacggta gcttggtcga
     8701 agcggtacgg ggcgtatagc cgccgccgct cgtcgatagc catcgcatga cgcgccgttc
     8761 ccacgttgtc gatcttgcgt gtaaacggcc agcgtgccgt ctcaaaacgg gccttgaagt
     8821 taagccagcc aacggttttg acggtgtccc acacgccgag gaaatgaact tgcggaaccg
     8881 cccgattgaa catggtgggg aaatcggggt tgccgaaaac tctcacaaac tcgtcacgtt
     8941 ggtcccagaa cttcttttcg gcggccttcg cctcagggcc aacttcggca cccggtccgg
     9001 ctttcgtata gagcttgacg gcgtagggca caaggttctc actccccggc cgcaataggc
     9061 cgacggtgcg aagcatcccg gtcagcgcac gggcggtgta cgcgccgcga gagaacccga
     9121 aaacatagat ccggtcgccc ttttggtagt tgcgcgacag ccaggtgtag gcctcttcga
     9181 ggttgtcctt gatgccgaag ccggcgacga ggcccgccac ccgggtcatt gccttgccga
     9241 accgggtcac cgacccacgt gcccccatcg tgccgacacc cgggtcgtag tacacgagtt
     9301 gattggcgtt cttctcggcg aggtcataga tccgggcaac gttggtagcc ccggtctcgg
     9361 gctcgttgtt ggttccgtcc aagcacagca cgaggtttcg ctctgtcgac tgtgggttgg
     9421 tcatcgctcc cccagtagct cgttaaccgt tgacatcaat cgttgttgag gttctacgct
     9481 cgcagccatg tcaacgccac ggcctaccct gcagactgat cccgaatggc cccacgaggc
     9541 aagcgatgcc gtcgaccgct tcgtggcggg tttgcaaacc gggatcgatt cggcggacgc
     9601 cgagctctac aacgcggact tcagcgacga cgtgatgtgg ggcagtccgt acggcgcgac
     9661 ggtcatcggc tatgacgcgt tgcactcgat tcaccgaggg atgttcgacc atggcgtagc
     9721 ggtggccggc gcgtcgtcgc gttacgagac ggtgcggata atggcgccga cgctagatgt
     9781 ggcgatcgcc caagttcgac gggtcgcgct ggacgccgac ggcgaaccga tcccgatcga
     9841 cgacccgtcg gtgttctcgg agatggcgat gtacgtgctc gtccgacgag acgggaaatg
     9901 gtggcttgct gccggccaga acacgccggt tcgtcccaag ccatgagcgc tgtcgccgag
     9961 cgacgagtct ttgccaaccg cggcaatgtg cacgcgcatt cccgtgaggc actccagtct
    10021 ctatttaccc accaggcagc ggcgtctcag caacgcaatt tgctgcagcc tggcgaatgc
    10081 tgtctggacc gccggaccat ggtttactgc cgtgcgtgga ttggttccag gaccatatcg
    10141 ccggcatctc cgccggtgcg tggacggcgc tcgccgcctg gctggccgtc gtcgccggca
    10201 tcgcagccct ggtctacgcc tggcgtcagt atcagcgcgc caaggagcag accgcggagc
    10261 tcatgcagcc caacgtcgcc atgttcatgg agcccagccc cggcgactgg cacctcatcg
    10321 agctcgtggt caagaacttc ggccgcactc ccgcgtacgg catccgattc gagttcgcca
    10381 atccgccgac ggtcggtaag tacgagagcg cctacgaaga tcgctacgtc gacatcacgc
    10441 cgctgaacct gcccgcggag atcccgtatc tcgcgccgtc gcaggagtgg cgcatcgtgt
    10501 gggattcggc actggaccgt cgcgaattgg gcgaggcgat cgcgtcgcgc ttcgacggtg
    10561 ccgtcaccta ctacgaccag cccaagggcg gcgcgcgggg caagcaaacc cggagccagt
    10621 accggtccac ggcggtgctg gactgggcga cgctgcaccc ggtcgagcgg ctggagctac
    10681 tgaccacaca cgaccttgcc cggcaggaaa ggcagaagct cgagctgctg cgcaacctgc
    10741 tgacctactt ccactacgcg gccagtgaga gccgcgaaga ggtgctgcgc accgagatca
    10801 accgtgtccg agcggcagcc gatgagctgc gcgagcggta ccgctcccag taccaggcgc
    10861 ccgtgaacaa caacttcgac ttcgacgagg cgcacaccac ggtgatcaac ggcaggcatc
    10921 gccgcgatct gatctagcac ggatcggcgg gctgtttttc gtaggcgatg tcgatctgtt
    10981 gttcgggccg ttgggcttcg gcgcgcaatg cgtcggtgta gtacgcctcg tcagccttgg
    11041 tgagcggggg ctgaatgatg cttctcggcc agaggtgctt gacgcgcacg acgtcggttt
    11101 cgatcgcgta gacgatgacc gtcgcctgaa gcgccagcca ggcgagcagt cccaagacga
    11161 cgccgaatag tccggcgacc gcctgcgcgt ggcgaagttg gtgggcgaca acgtatcccg
    11221 cgaggatgag caacagctgc cagccgaccg cgctgactgc ggcactcagc agcagagacc
    11281 gcgtcggcac ctgcgccgcg gccgcaacac gaaacagagc gaggaaaaac ccgaagccga
    11341 ggatcgtgcc gaccgcgaaa ctgacgaacc gcgctggcat gccgccaagg ccgacggacg
    11401 tcgcgacgcc ggcggcagta ctggctgcgc cggtgaccat gacgatgagg ccgaggagca
    11461 acagcactgt gatggtgcgc agatagcgcg gcgccaaccc cggccggtcg gtcttgggca
    11521 cggcccacac ggtgttgagg gtgttctgca gggcgtgggc gaagccgcgg ccaccgagga
    11581 tggccaccac gattccggcg gtcaatgcga tcgcggtacc gctgaatttc tccacgccaa
    11641 gttgttcgtg gatctccgcg ccgacgatgg gaaactcggc gaatgcggag ttgacgagtt
    11701 tcgcgtgcag ttccggatac ttgcgcaacg ccacacccag caccgcggtc agcgcgagca
    11761 gtagcgggaa ggtcgcgagg aaaccgtagt acgtcagcag cgcaacaaga ttgcccgcct
    11821 cgtcgtcgcc gaacttcttg accaccgcca ccgggaaggc cgcaatgggg tggcgctgct
    11881 gccagcagtc gacgctacgt acggcccgct ccacggggtt catcagtcag cgctcgcacc
    11941 gtcgtcctta gtgttggcgc tggagtaccc gcattcgggc ggcgtgtcac ttccagacgg
    12001 agccgatgcc gcattcccaa aggctgagtt tgggcacgcc gctgttgcgc ggttgcttgc
    12061 gacgcttggt ggtcttaccg gcttcggact cgagatgttc ggctgaccgc ttccgcattg
    12121 atcgcatggg tgtccccctt gggcttatgc cggcgacccc tcgccgccgg catgaattaa
    12181 cgctatggac gggctaggtg atggacacgc gtacaccact acgcgaatct ggggttggtt
    12241 ttcgtaacgt tgttacgtaa catcgttagc catggctgac accgtgaaat acgagctcaa
    12301 cgatgccgtc gcgaccatca cgctggacga cggcaaggtc aacgtgctgg ggccggcgat
    12361 gcagtcggcg atcaatgagg tgctcgacaa ggcggagaaa gactcggcca aggccgtcgt
    12421 gatcgcgggc aaccagcggg tgttttccgg cggcttcgac ctggcggtgt tccagtcggg
    12481 cgacgcgaag gccgcgctgg gcatgctcgc gggcggcttc gagctgtcgt tacggtgcct
    12541 gacgttcccg gtgccggtga tcatggcggc gacgggcccg gccatcgcga tggggtcgtt
    12601 cctgttgctg tccggcgacc accgggtggg ccagcctaag tcgcggtgcc aggcgatcga
    12661 ggtggcgatc ggcatgacga tcccaatctc ggcagtcgag atcatgcggg cgcggctgac
    12721 gcctgcggcg tttcaccggg gcgcctcgat ggcggcgacg ttcgccggtg acgaggcgct
    12781 ggcgggcggt tggctcgatg agctggtgga ggccgaccgg gtgctcgcgc gggcgcagga
    12841 ggtggcggcc gaagcggctg caaccttgca cgcgggcgcc cacctcgcga ccaaactgaa
    12901 ggcgcgcgat tccgcgttga aggcgataca ggccggaatc gacgggcttg caacggaatt
    12961 cactctcggc tgatgccaag ggccaagcag cgcacgcccg agcttcggga tcggctgctg
    13021 gaagtcgcga tcgagacgct gtccgaggaa gggatcgcgc ggttcaccac acggcgggtg
    13081 gccgaacggg cgggcacctc agtgcccgcg gtgtacgagc tgttcgacga caaggccggc
    13141 ctggtgcggg cgatgttctt cgagggcttt cggctgctgg gcggcgagct ggccaaggtg
    13201 cccgtgacga cggatcccgt agcggatctg gagcggctgg tgccggtgtt ccggttgttc
    13261 tgccgggcgt atccgcggct cgcgcaggtg atgttctcgc ggccgttcgc cgacttcgag
    13321 ccgggtcccg aggagctggc ggccggcgcg tcggtgcgcg aggtgttcgt cggacggatc
    13381 gagcgatgca tcgacgcggg tctgctgtcg ggcgacccga acgacatggc gcacgtattg
    13441 ctcgctctgg cacagggttt ggcggtgcaa gaagcgggcc ggtggctcgg gtcggggccg
    13501 ttgatcgagc ggcgatggaa gctgggggtg acgtcgctgc tcgccgggct gcgataattc
    13561 tcctatggcc ggtcgcatcg gctcgatgtt cgggccgtac gagctgcagg cgctgctcgg
    13621 cgtcggtggg atgggcgaag tttatcgcgc ctatgacacc tccaaggatc ggacggttgc
    13681 gctcaaactg ttgcgcgccg agatgtctgc ggacgccggc tttcaggagc ggttcaggcg
    13741 cgagtcgcgg atcgcggcga gactgcagga gccgcacgtc atcccggtgc acgacttcgg
    13801 cgagatcaac ggtgtgctct acatcgacat gcggctggtc gagggcaaca acgtgcacaa
    13861 cgtgctgcag cagaacgggc ctttggatcc ggcgcgtgcc gtgtcgatcg tggggcaggt
    13921 ggccgcagct ttggacgccg cgcacgccag cgggctcgtc catcgcgacg tcaagccgga
    13981 gaacgtccta ctcacccata ccgatttcgc ataccttgtc gacttcggca tcgcgcacct
    14041 cggcggcgac accggtctga cgagcgccgg cgcggccatc gggtcgtgcg catacatggc
    14101 gccggaacgg ttcacgggtg gaagggtggg cccggcggcg gatgtgtact cgctggcgtg
    14161 cctgctctac gagtgcctga ccggttcggc gccgttcccg accggcgaat tgagtcagct
    14221 gatgggcgct cacgttatgt cgccgccacc gcggccgagt gcgacgcggc caatcgtcgg
    14281 gaccgcattc gatggcatcg tcgcgcgcgg aatggccaag caacccgagg agaggttctc
    14341 gtcggcgggt gatctggctc gggcggccaa tgccgcggtc atggcagctc aggtgccgac
    14401 ggcgttcaac cgcttgacag caccgcctcc tcctcctcaa agtccgcccg tacgaagagg
    14461 tttcggacgc gtgcaatggc tcctcgccgg tgcggcgttc ggaaccctcg cggcagcggt
    14521 gggcgtcgtg ctgtggcttg tgctcgggca ggacaagacc gagcacgatg tggcgcctcc
    14581 gacgactact agtgccgcgc ccccggcgac gacgagcgcc gagccgccga cgacgagccc
    14641 gccgacgtcg acgtcaccgg aggctccggt caccttgccc ggcaccgacg cccagggctt
    14701 catcgactat cccggagcgc ggtgcgatgc gggcaccagc ccggcggcgc tggcccgaac
    14761 gactcagtcg gtgtttgtgg tgtgccgttc cgggcctggc gacttctact accgcggggt
    14821 tcggctgagc gacggcgcgg gcatcgaact cgcgaacgcc gtgcgatcgt cgggcggatt
    14881 cgacgtcacg aacccgacgg acggcacccg gtatcagatc cgtccggacg gcttgacgat
    14941 catcacgccg aatggtcaga cgttcaccga gccgatggtc gcgtacggga cgagctgacg
    15001 ccgctgcgcg cccgctacag cccaccgcac cgcgagggcg ccaagaaggg cgagtgccaa
    15061 gggcaatccg gcggcaatgg ctgcggcgga ggcggcgtat agggcgccac gaccggaggc
    15121 ggcggcgcat catgcggagt gccacgccag gggtagatga tgttggcggc cacgtcgacg
    15181 acgccctccg aattccagta ccagtcgtgg cagacattcc agtcccagct gatgacctgg
    15241 ctgcccggga tcaagggatc gcctggacac cagtgggtgc aggtatatgc ggtgggacag
    15301 tttccaccgt tatagctagg cccccaggct gggtcggctt gcgcggctcc cgcggccaga
    15361 cccaagccgg ccacggtcac cccgccagat acgacgaccg cagttgcgaa tcgtgaaact
    15421 cggctcatcg gttggttcct ttcatgaggc ctccccgctg gcactgcttc tgcggtcaag
    15481 tagagaccgc gagggtcact accgatccca acgttccgac agaggccttg gcgaattctt
    15541 ggagccgccg cgatgatgaa ggggtgacgc acgtccgagg ccgggtctgg cgggccggta
    15601 agccgcagga cgatttcgac ttcccgtcca tctcggacta cctggccgaa gacgacgcac
    15661 tggtgtggtg cgacatctac gaccccgacc acgcgatcct gaaggacctg gcggaggaac
    15721 tcagcctgaa cacgtgggcg gtcgaggacg cgatcgcgga atccgaacgc accaaggccg
    15781 tcgtctaccg cacgcacacc ttcttcacgg tgtacggcgt caccaccaag gagacgacgg
    15841 atgagacgga gtcgatgctc gacatccacc gcatctccgg gttcgtgctg ccgcgcgggc
    15901 tgatcacggt gcggctctcg ccgcacttcg acatcgaagc ggtgtcgcag cgcttcgacg
    15961 agctcggtgg ccaggagcac ggcgtcggaa cattggtgca cggtctgctc gatgtggtgg
    16021 tcgacgggca cttcgaagcg gtgcaacagc ttgacgacgg catcgagtcc atcgaggacg
    16081 acctgttcga gaacaatccg cgaagcggcc tgcagcgcaa gacgtttcgg ctacgaaagg
    16141 acctcgtcga actgcggcgg gtggtgttgc cgatgcggga agtcgtcaac tcgattcagc
    16201 atcggcggct ggacgccaag acggcgccgg agctggaccc gctgtattcc gacctctacg
    16261 accacgtgct gcgggtgtcg gagtggacag agtcgttgcg ggacatggtc acaacggtgt
    16321 tcgagacgaa tctttcgctg caggacgccc ggcttaacac ggtgatgaag aagctgaccg
    16381 gctgggcggc gatcatcgcg gtgccgacgg ccatcaccgg gttctacggg cagaatgtgg
    16441 agtatcccgg catcaatacg ttctggggtt tcctggccag cacgtcgatc attgtgctga
    16501 tcgtgctggc gctgtactgg atattccggc gacgaaactg gctgtagcac aacctgtgca
    16561 gacgcgatgc tggcggttcg atgagaaaac acggccacgt taggtggatc ggcccaactc
    16621 gcgtagggtg aagacaccgg ttgagttccc agccgccgga aacgtcgcag gtcacacgtg
    16681 taaacccact gctgtgaggg cctttttgac gcaaccttgt ctcttgcccg gctgtcacgt
    16741 ttggagtcga ccacctatga ccactatgac tacaccattg ctcaccacga cgacccgcgc
    16801 tgagttgaaa ctgtccaccg aatggccgaa ttccactgtc gtgcgcatcc gtgcttccgg
    16861 tgatatcgac gcatccaatt ccgccgaaat gctggactac gtgctccgcc gcggagcgaa
    16921 ttgccggagc ctggtcctgg acctgagcga cgttactttc tttggcaccg ccggcttttc
    16981 ggccctacag acgatcgacg tccgtagctc acgcgcatcg gtcagttgga tgttggttgc
    17041 cgggcctgcg gtttcgcgcg tgctgagcat ctgcgatccg caacgcgagc tccctcgcgc
    17101 ggtcgcataa cccgctagat catcattggc gcgtgcgacc ggacaagccg gtcggcaagc
    17161 cggtagtcgt cgtccgcatt gagcgtgagg acgccgacga gatcgccgca cgattcgtag
    17221 tagaccgtga acccgttgtg gtggtcgacg agtcggctgt gttcgtagcc gagaccccag
    17281 ccgcggtact tcagcgtgga ttcgcctatc gtgcaagaga atccgggcac cgtgtcccat
    17341 actgcgggct ggccggccgc ggacaacccg gcgatgaggc cctgctgtgc ggcgtcgcgc
    17401 cagtgctcgg cgcggatgcg ccggccggcg gtcacgttgt aggcgagcgc gacatcgcct
    17461 gcggcgtaga cattcagcgc cgaggtgtgc atgcgttcgt tgacgacgat gcggccgtcg
    17521 tatgtgtcga ggcctgccgc ctcggccagg tgcgggtcgg ggcgcacgcc ggaggcggag
    17581 acgacgacgt caccatccag tgcggcaccc gtgtcgaggt ggacgtgcgt gtcgtagatc
    17641 gcggtgacgg tagtcgaccc gaggaagcga accccgttgt cggacaggat ctttgccact
    17701 cgctcgccgg cctcgtcacc gaaccggcgc tgcagcggca cgggctcggc ggcgaccatc
    17761 gtggtcggga cgccgcggct ggccagacac gcggccgcct cgcagccgat cagcccgcca
    17821 ccgattacga cggccgagtc ggcgctgcgg gcggccatcc ggaggatcac tgcgtcggcg
    17881 aaggagcgca ggggcagcgc ggcttcggcg ccgggaatgc tcagcgggac agggaaggcg
    17941 ccgca
//
LOCUS       DASRJA010000462        22162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3369285,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000462 DASRJA010000000
VERSION     DASRJA010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22162
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3369285"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFC01_20275"
     CDS             complement(<1..194)
                     /locus_tag="VFC01_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN81998.1"
                     /translation="MTSGGRIRLRLDAIRPAVLIEPVTSELSLEGELDQANRVGHEAA
                     GDKCERLRRGLIEPLGVIHHT"
     gene            complement(191..784)
                     /locus_tag="VFC01_20280"
     CDS             complement(191..784)
                     /locus_tag="VFC01_20280"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZN81999.1"
                     /translation="MQIGILGAGNVGVAVGRRLTGAGHRVMLSSARGPVALAPVAQSI
                     GAAATTVTEAAAQELVLLAVPWLAVPETLDALPDWQGRILVDATNPFVTFDPLQLADL
                     EGRSASTVVAEHAPGARVVKAFNSIAMSNFEKGPRQGDAKRVLFVSGDDVASKQTVKS
                     LIAELGYAPLDLGSLDDGGRLQQAGGPLAALDLLVNP"
     gene            complement(1083..2003)
                     /locus_tag="VFC01_20285"
     CDS             complement(1083..2003)
                     /locus_tag="VFC01_20285"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN82000.1"
                     /translation="MKAVGVTGPAYMPRLFDIDEPVASRGGVVVEVLAASVNDFDRAA
                     VEGRVALTSQLDPVLLGRDFVGRVTAVGHDVDYIDVGMNVAGTMEAWRSGTFTDSVEV
                     PAELLAPVPDGVDVAQAAGVGLAAITALDAVGVLGSTNLGTTVIHGPVTEVGGFAIQL
                     AKARSAVVAVVTPSAQADLAWALGADVVITEGADATQTIQGVRDFFGGGVDNAIHLAG
                     DPSIAARVVRPGGKCTSVSDAATPAIRTDVEYIPTVVAPSGHKLADLLFKVAAQRLHS
                     NIGRTFSFDEAGDAVNADHTSAGGRTVLVR"
     gene            2103..2597
                     /locus_tag="VFC01_20290"
     CDS             2103..2597
                     /locus_tag="VFC01_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN82001.1"
                     /translation="MDTANTTRWLDEEEQLTWRQLSTMILRIQPVLSAQLQREFGISH
                     FEYLIMACLSEAPGSKLRMSVLAAMTGSSLSRLSQAVGRLEKRGWTSRQSDEQDSRFT
                     LAVLTSAGLRQLQEAAPSHVAAVREFVFDRLTQAQSRQLGTICQVILDGLPPDDSWPN
                     IPAP"
     gene            2807..3625
                     /locus_tag="VFC01_20295"
     CDS             2807..3625
                     /locus_tag="VFC01_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN82002.1"
                     /translation="MGKLSVRATLSTILVALALTAMLAGSFGAPPSAKAADDSCPAVE
                     VVFARGTNEAQGVGATGQSFVDALTARLPGKTVDVYGVNYPASMNFGQAADGIVDASN
                     KIQSIAASCPSTQIVLGGYSQGAAVAGYTTTDSVPAGFDLPAGITGPMPPAVASHVAA
                     VVLFGTPDAWIMNLLDRSAPPITIGQSYIAKTLQLCATGDPVCFPGGLDRSAHSSYKN
                     NGMADQAAAFAVGQLKGGAPAATMQQTAGEVGAGQPAAERAPAPEPAVDAPVGN"
     gene            complement(3713..3916)
                     /locus_tag="VFC01_20300"
                     /pseudo
     CDS             complement(3713..3916)
                     /locus_tag="VFC01_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
     gene            complement(3962..4957)
                     /locus_tag="VFC01_20305"
     CDS             complement(3962..4957)
                     /locus_tag="VFC01_20305"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN82003.1"
                     /translation="MARAITYAAAGNPADVLEVTEIPEPPEPGPGQVQVEVRAFPIHP
                     GDLVGVGFGPPHPGQRTVAGLEATGVVVATGTGVETPAVGTRVTVFPNPGSWAQRINV
                     ATEVAVAVPDSVSDDVAAQMICNPLTALLLYRAAQQHFSIGFGGIIINNAATSSVGRL
                     FTASADHHQIATISVVRSADRARELAARYPNVAVVSTDNPDWVQQIRTIAEGRPIPVA
                     LDPVGGAVAGHLISLLSSGGTLITYGAIAQDTISLHASALLGGEIGIRGLTVGRWLSG
                     VAAERRASDIASVIAIAIGLSAEFDVAGTYTLDQISDAVRHVNRPGKIGTIIVKT"
     gene            5147..5800
                     /locus_tag="VFC01_20310"
     CDS             5147..5800
                     /locus_tag="VFC01_20310"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HZN82004.1"
                     /translation="MRELGFAVSDRWLIRGTEFTNCNCAWGCPCQFGAKTTHGHCETV
                     MCGHIEEGNFNGTNLDGLNWAVLMYWPGEVAECNGTQQAIIDERADPPQREGLRKILH
                     GDSTAPGATHFYVYNSTMSTVLDTLYAPIVLSIDVDQRMASLKIDDLVESRGTPIISP
                     HTGEPTRSRINMPDGIHYIFAEMGNGNTKARAGVELDLKDTYGQFNVLHVNQDGMIR"
     gene            5833..6591
                     /locus_tag="VFC01_20315"
     CDS             5833..6591
                     /locus_tag="VFC01_20315"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HZN82005.1"
                     /translation="MAVLAGLGSITVAAWIYVVVEARRMASESADMGGRPMASMMESM
                     TGLQPWTATEFALRLAMWAVMMVAMMVPTAVPMTLLYAAVARKAAAQHNPLASTSVFV
                     SGYIAMWTIFSLVATIAQHALDQAALLSPLMVTRSAGLGATLLIAAGVYQLTPLKNAC
                     LRNCRAPAHFLSRYWRTGDLGAFRMGLRLGAYCVGCCWILMGLLFVGGVMNLLWIAAI
                     AIFVLLEKTSPFGDVSGRFAGVALILVGAVSLAL"
     gene            complement(6613..7017)
                     /locus_tag="VFC01_20320"
     CDS             complement(6613..7017)
                     /locus_tag="VFC01_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZN82006.1"
                     /translation="MNYEINRQLQRDNELSLADFHVLCALSIAPNRKLQISDLAAVIC
                     WERSRLSHHLRRLCDRGLTERLQSEVDARATNVVLTEEGMSAIVAAAPGHARLVRRLF
                     FDPVPDELLSPFTAALEHIQASLNSNSSLPPG"
     gene            7294..8220
                     /locus_tag="VFC01_20325"
     CDS             7294..8220
                     /locus_tag="VFC01_20325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005100517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZN82007.1"
                     /translation="MARAVQFDSYGGVDVLEVRDVPRPAPGAGEVLIEVKAAGINISE
                     AAIRAGAVRDMFPATFPSGQGSDLAGVVAELGSGVKSFAVGDEVIGFSNLRSSHAEYV
                     AVPATQLTPKPAAVPWEVAGSLFVAGATAYGAVRAVRLAPGETVAIAGAAGGVGSIAI
                     QLARRAGATVLGIAGPSNDAWLTDHGAVPVNYGVDLPARLRAAAPSGRIDALLDFFGD
                     GYVAMAVEDLRVPRERVDTIADFAAVERFGVLSAGGADAATAAVVAELADLIAQGQLD
                     VPIAGVFALDDVRNAYRELEKRHTRGKLVLRP"
     gene            8240..8668
                     /locus_tag="VFC01_20330"
     CDS             8240..8668
                     /locus_tag="VFC01_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN82008.1"
                     /translation="MSDGNDFNERNIAEFRANHGRVSGPFEGAPIVLLHTVGARSGKP
                     RTSIMMYLADGDRYLVFASSAGADQNPAWYWNLKANPHVRIEIGDDIVDVHATELQGA
                     ERDEKYAIQAERYPGFAEYERMTSRTIPVIALTPTGGARS"
     gene            8701..9345
                     /locus_tag="VFC01_20335"
     CDS             8701..9345
                     /locus_tag="VFC01_20335"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN82009.1"
                     /translation="MPRMDFDALYRGESPGEGMAAMTTPPWDTKEPNENVIAWQTGGW
                     VHGDVLDVGCGLGDNAVYLAKNGHAVTGLDISPTALITAEQRAKDAGVDVTFAVADST
                     KLDGYTDAFDTVIDSGLFHSLDDDGRRSYAAAVHRATRPGATLLLSCFSDANLVGQEW
                     PRPAVSEETLRDVLGGAGWDIVSLEAATVRGALDGGPQAEMAFWYLRAQRRQLV"
     gene            complement(9408..9896)
                     /locus_tag="VFC01_20340"
     CDS             complement(9408..9896)
                     /locus_tag="VFC01_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN82010.1"
                     /translation="MTSGDFRRAAHVDLSLQEMADRVAIRELVDTYAFCADRRDATGQ
                     MALFTEDTDFQVYMDTSSSIPAQHIQGRTALAPVFDELNGYEATMHFNGQSTTMLDGQ
                     NATGIAYCLAHHVKVEAAGRSLMIAAIRYLDTFVKQDGIWYFSQRKLMVDWTETRALV
                     TG"
     gene            10067..10444
                     /locus_tag="VFC01_20345"
     CDS             10067..10444
                     /locus_tag="VFC01_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN82011.1"
                     /translation="MSPISATISELMHANLLEVFNERDRVRRSAAIARIYADDIRWTD
                     DDGVTIGHAALDGKAAELQSKLGDLQFIAAGPTYQTLGLGYLAFNVVKPDGGAPQISG
                     FDVAIVRDGLITELYTVLTDLPR"
     gene            10643..11206
                     /locus_tag="VFC01_20350"
     CDS             10643..11206
                     /locus_tag="VFC01_20350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZN82012.1"
                     /translation="MTELTIAIIAGSTRPGRHAGAVSEWILKQAAERLGVRYDIVDLI
                     DHPLPLLDEAMPPSFGQYQNEHTKAWAATIAPYDGFVFVSPEYNHSTSAALKNALDYL
                     YAEWQNKAAAFAAYGSLSGARAVEHLRLICSELQMATVRQQLSFSLFTDFENFTAFTP
                     AALHNDSAAVMFDQLESWAGALKTLRS"
     gene            11280..12158
                     /locus_tag="VFC01_20355"
     CDS             11280..12158
                     /locus_tag="VFC01_20355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZN82013.1"
                     /translation="MSTLTGDRYRLGDKVVHRVGYGAMQLAGDGVFGPPRDRDEAIAV
                     LRAAVDAGVDHIDTAQYYGPGVVNQLIREALSPYPDRLALVSKVAARRDDFGAVLHYD
                     DPDQLRAGIEDNLATLGVEQLTAVNLRVMDNAVPDERFVEQIGALVAARDEGLIAGIG
                     ISNVTHRHLRRAVEVTEIVCVQNAYNLADRSSEPVLKECTTRGIAFVPFCPLGWPKSQ
                     HDAIRTNPIVTGIAAGHGATPAQVALAWLLAVAENVLLIPGTSTRAHLAENLAAGSLS
                     LRDDEISSLSSAFGVR"
     gene            12397..13395
                     /locus_tag="VFC01_20360"
     CDS             12397..13395
                     /locus_tag="VFC01_20360"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZN82014.1"
                     /translation="MPAVVLTAEREIALEERPRPSLRSDQVIVEVELCGICGSDLHSA
                     QMPQVYHGDCTLGHESTGHIVAVGADVAEWAVGQRVAINPNGNVCGTCKFCRAGQSNF
                     CVQATLETALGLQADGGLAPLMAAFPSHLRAIPRGLASREAAWVEPAATALRAVERAG
                     DLQGRTVLVTGGGPIGQLACRLVNLESPERVLLMEPAVDRAEFASNSHATVIAADEAT
                     ESDTRVDIVIECSGNETATASALSLLTPGGTLVVVGGGRGSGLDPTTILLKEITVHGS
                     YTYIGEFDRAIDLLATGQLAVADLTSVVTPLSDTLSAFDSLRAGRIMKALIAPSQH"
     gene            13425..13805
                     /locus_tag="VFC01_20365"
     CDS             13425..13805
                     /locus_tag="VFC01_20365"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN82015.1"
                     /translation="MTDTPPNNVDLVRTLIHAVDNADNDTIAACTAKDVHFRFGNAQP
                     TTTQSELLAAAGSFRGGIAALRHTILNMWEVDGGTVIATMDVYYRRLDGGELNLPCCN
                     IFRVEDGRVVDYLIYMDVNPVIAT"
     gene            complement(13933..14439)
                     /locus_tag="VFC01_20370"
     CDS             complement(13933..14439)
                     /locus_tag="VFC01_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZN82016.1"
                     /translation="MSPARRSRSGDNPWLPASQKRAWVAFMRVQLRMNYEMNRQLQRD
                     SGLSLADYHVLNQLSMAPDHKLQITELAALIGWERSRLSHQVRRLCERGLTERIQSED
                     DGRATDALLTKKGLEVIVAAAPEHVALVRKLFFDPLPDDLVNPFTAALEHIQASLNLN
                     ASLPPVPL"
     gene            complement(14708..14857)
                     /locus_tag="VFC01_20375"
     CDS             complement(14708..14857)
                     /locus_tag="VFC01_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82017.1"
                     /translation="MKRDVDTVLTYPAVMGSVLLLALQVGVSLGLAVVAACVAWPASW
                     WYWRR"
     gene            complement(14854..15741)
                     /locus_tag="VFC01_20380"
     CDS             complement(14854..15741)
                     /locus_tag="VFC01_20380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005139995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN82018.1"
                     /translation="MTTPNIDLGRFGVFTWDPGSSFLDLGLAVTPEQAQEIERLGYGT
                     LWVTGSPAAELSFAEPLLESTTALKVATGIVNIWTADAKTVAESFHRINTAHPGRFVL
                     GIGAGHPEHTEQYRKPYDALVDYLDELDEYGVPANQRVLAALGPRVLRLAAGRTAGAH
                     PYNTTPEHTAQAREIIGPNALLLPEHKVLPIADAEEARAVGRKVLNTYNYLNLTNYLN
                     NFKRLGFTDADLTPPGSDEFIDALVAYGTPGDIANRLGEHLRAGANQVVIQVLGGSDK
                     LLPTLTELAGPLGLKASAA"
     gene            complement(15758..16171)
                     /locus_tag="VFC01_20385"
     CDS             complement(15758..16171)
                     /locus_tag="VFC01_20385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZN82019.1"
                     /translation="MVAVPAGYNDLLELPLYGHLATARPDGSVQVSPMWFDWDGELLR
                     FAHTTKRQKYRNVQSNPRVAMSISDPDNPYRYLEVRGDVERIEPDPAASFFRRIAKRY
                     SGDEPPPDAPPRGEAPSDAPDWVVIVVRPTGFSRH"
     gene            complement(16289..17014)
                     /locus_tag="VFC01_20390"
     CDS             complement(16289..17014)
                     /locus_tag="VFC01_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN82020.1"
                     /translation="MARLAGKVAVITGGSSGIGLATAQRFVDEGARVFIMGRRRTELD
                     KAKALIGDGLSTVAGDVTSNADLDRLFLTVLNAEGGLDILVANSARAETEMLGKITEE
                     NFDATFNVNARATLFTVQKALPWIRNGGSVILIGSTSGHMGVSGYSAYSATKAALRSY
                     TRTWTREFKDRGIRFNTLSPGPIDTPMLDGQADKAQFVAAVPLNRLGRPDEIASAALF
                     LASDDSSFVAGVELSVDGGMAQV"
     gene            complement(17225..18055)
                     /locus_tag="VFC01_20395"
     CDS             complement(17225..18055)
                     /locus_tag="VFC01_20395"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN82021.1"
                     /translation="MTTTAESVVARWARSGQPLCVGIIGLGAREDLWACLAHLPALRA
                     IPGFEVTALSTSSPESAASAGEKHGVARTFGNATDLAACDEVDLVVVSVRVPAHRELV
                     SAAVQAGRAVFCEWPPGNGLAETEELARLVGEHDVPSIVGLQIRTNPVMNYLRDLVAD
                     GYVGEVLSNGTEGDIVVTAPIGHIQLAPLTLLGARGDAAELAVLPVPQSYRKVASLDW
                     PATGGGLGVADFYRQGAFGVAHLYQQFLEDLREGGNSVPDFSDAVVRHLSIHNASNMP
                     "
     gene            18105..18212
                     /locus_tag="VFC01_20400"
                     /pseudo
     CDS             18105..18212
                     /locus_tag="VFC01_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(18309..18671)
                     /locus_tag="VFC01_20405"
     CDS             complement(18309..18671)
                     /locus_tag="VFC01_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN82022.1"
                     /translation="MKEIDLTGIVAEHVDAINAGDTDRAVATFAEDAYVNDNHREFVG
                     IDAIRRWVAREMVGDKVTIEVREVLDHYGDTVVRGAYDGTFDRTNLPDEIVLTNYFSV
                     RDGQIVSLAIVFNQPADY"
     gene            18804..19634
                     /locus_tag="VFC01_20410"
     CDS             18804..19634
                     /locus_tag="VFC01_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZN82023.1"
                     /translation="MSSPALLAIGDFSRATQLSVKMLRHYHQVGLLEPVDVDRATGYR
                     RYSIDQISTAQIIRRFRALEMPLDDIRSVMSTTDIRRRNELINAHLMRLESQLAQTKR
                     AVESLRELLEPGPVAEGRIGYRRVEATEAIAISEVVDISDALTWVPGALAELYATLTA
                     QGVRICGPAGGIFADELVASERGQATVFVPFGGDFRRTGRLTELVVPAAELATVVHAG
                     SYRDFDLAYGALATHVAQHAIAVDGPIREYFLVGPHDTSDETAWRTEIGWPVFHTTTG
                     "
     gene            complement(20307..21065)
                     /locus_tag="VFC01_20415"
     CDS             complement(20307..21065)
                     /locus_tag="VFC01_20415"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZN82024.1"
                     /translation="MRRAHGDVMAVQSKTILKGGLHLAVMANVIGANPVRDVSPMRST
                     SRPKGAPALTAGELRALLGKLRASEACQRHDLVDPITLFIATGLRISELLGLYWTEFD
                     DTAPTLTITGKVIRAAGKGLMRVDETKTAAGTRTLPLPTFAVAVLAARRGRAYLGEQP
                     MIFPSTAGTWRDPDNFRARWREVREELGVPDVTSHSFRKTVATLIDDEGLSARIGADH
                     LGHARVSETQDTYMARGRSHTAVAELLDRTISDE"
     gene            complement(21041..21364)
                     /locus_tag="VFC01_20420"
     CDS             complement(21041..21364)
                     /locus_tag="VFC01_20420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82025.1"
                     /translation="MTRIVERRGPADEHDQHRKLAEDALVEAIMQRRATGDGGVTLDT
                     KIMALVDRHIDRLEEDGRAVRTIDTYRYCAKLLSKIIAGVRVGRRRRPESTRPSARCA
                     GPTAT"
     gene            21610..>22162
                     /locus_tag="VFC01_20425"
     CDS             21610..>22162
                     /locus_tag="VFC01_20425"
                     /inference="COORDINATES: protein motif:HMM:NF019842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HZN82026.1"
                     /translation="MHRLLRGLAVLAALPLIALPTAEAATVLMISPLTTNMQDELQGS
                     MCETNTCVVVDAPAYPDPRGVIALDNAIKSTPGEIIVFGYSNGAQIVSQWLTQHANDP
                     TAPSPDRLSFVLIGNPTRAYGGTSAPMPQTKYLVTDIAREYDVAADYPNNPKSRYYSL
                     AVNNAWAGFFSIHANYWDVNQYDP"
BASE COUNT         4085 a         7022 c         6871 g         4184 t
ORIGIN      
        1 gtatggtgga tgacgcccaa tggctcgatc aagcctcggc gcagacgctc gcatttgtcg
       61 cccgccgcct cgtggccgac tcggttggct tgatcgagct cgccctcaag ggatagctca
      121 ctggtgaccg gttcgatgag cacggcgggg cggatcgcgt cgagcctcag tctgatccga
      181 ccaccactcg tcatgggttg acgagtaggt ccagggctgc gagtggacca ccggcctgtt
      241 gcaaccggcc gccatcatcg aggctgccga ggtcgagcgg cgcgtagccg agttcggcga
      301 tcaggctctt gaccgtctgc ttcgacgcga cgtcgtcacc ggagacgaac aacacacgtt
      361 tcgcgtcgcc ctgtcgaggc cccttttcga aattcgacat ggcgatggag ttgaacgcct
      421 tgaccacccg cgcaccgggt gcgtgttcgg cgacaacggt gcttgcgctt ctgccctcga
      481 gatcggcgag ctgcagggga tcgaacgtga cgaatgggtt cgtggcgtcg accaggattc
      541 tgccttgcca atcgggtagc gcgtcgagtg tctccggcac cgccagccag ggcaccgcaa
      601 gcaggacgag ctcctgcgcc gcggcctcgg tcaccgtggt ggccgctgca ccgatgcttt
      661 gcgcgactgg cgctagcgcc actgggccgc gcgcactgga aagcatcacg cggtggcccg
      721 cccccgtcag ccgtcgcccc accgccacgc cgacgttgcc tgctccgaga attccaattt
      781 gcatgtctat ctcctaagac tcatttctga tacaccaccc acgagcgtcg cacttcgacg
      841 tctggattgt tcaaccggac tttggtccgg cttatttcct ttgtttccat ggcttttggt
      901 atatctcgag agtccgaaca ctaaattgga cttgtccaac atggcgtggc gtcatcacga
      961 aacgttcccg tgctcaatat atttggtggg atccggggaa cgtagcatgt gtcttcgagc
     1021 tgcgcacagt tgccaattct tcatgctgac actagctttc gcgactagat tgcagcgacg
     1081 gctcagcgaa ccaggacagt acggccgccg gcgctggtgt gatccgcatt gacggcgtcg
     1141 ccggcctcgt cgaaggaaaa tgtccggccg atattgctgt gtagccgttg cgcggcgacc
     1201 ttgaaaagga gatcagcaag cttgtgcccg ctcggagcga caaccgtcgg gatgtactcg
     1261 acgtctgtgc ggatagcggg cgttgcggcg tcagagaccg aggtgcactt cccacccggg
     1321 cgtaccacgc gggcagcgat cgaagggtca ccggcgaggt ggatggcgtt gtcgacgccg
     1381 ccgccgaaaa agtcccgcac cccttggatt gtctgtgtcg cgtctgcgcc ctcggtaatg
     1441 acaacgtcgg cgccgagcgc ccacgcgagg tccgcctgag cagacggggt aacgacagcg
     1501 accacggcac tgcgggcctt cgccaactgg attgcgaacc cgccgacctc ggtgacggga
     1561 ccgtggatga cggtggttcc gagattcgtc gacccgagga cgccgaccgc atcgagggca
     1621 gtgatcgccg ccaaccccac gccggcggct tgggcgacat caacgccgtc agggactggg
     1681 gcaagcaatt ccgccggaac ctccaccgag tcggtaaacg tccctgatcg ccatgcctcc
     1741 atcgtcccgg cgacattcat gccgacgtcg atgtagtcca cgtcgtggcc gacggctgtc
     1801 acccggccca cgaagtcacg gcccagcaga acgggatcga gctggctcgt caaggccacg
     1861 cgtccctcga ccgcggctcg atcgaaatcg ttcaccgagg ctgccagcac ctccacaacg
     1921 actcccccgc gtgacgcgac tggttcgtcg atgtcgaaca agcgcggcat gtaggccggc
     1981 ccggtcactc caacagcctt catcgccgac cctccttatt gagcgcgttc gatccgatgc
     2041 acggtttaac aatgaagtgc tgcgattatt cctcggttca atctttaagt tagaatgaga
     2101 tcatggacac tgcgaacacg actcggtggc tggacgagga ggaacagctg acctggcggc
     2161 agctgtcgac gatgatcctg aggattcaac cagtgctcag cgcacaactg cagcgcgagt
     2221 tcggcatcag tcacttcgaa tacctgatca tggcgtgcct gtcggaagcg ccgggatcga
     2281 agctgcggat gagcgtgctg gccgcgatga ccggcagctc actgtccagg ttgtcccagg
     2341 ctgtcggccg cctggagaaa cgtggctgga cgtcacggca atccgacgaa caggacagtc
     2401 gtttcaccct tgcggttctg actagcgcgg gacttcgcca actgcaagaa gccgctccgt
     2461 cgcatgtcgc cgcggtacgg gagttcgtct tcgaccgatt gacacaggcc cagtcccgtc
     2521 agctgggcac gatttgccag gtcattctcg atgggctgcc gccagatgac agctggccca
     2581 acatcccggc gccctgaact ctccaacaac acccccatta tcccggcgtc gtacctgctc
     2641 agcgaccatt cttccgcaag tgacgtagat tactagattt cccggaatcg cagctgcggc
     2701 ctgcaacttg taaggggcag gcagtcactt aaacattgaa gtgacagtga ctggaaaccc
     2761 cgaataggag ccgatcatga gcaccgtcac accaatggtc gtcaggatgg gcaaattgtc
     2821 cgtccgcgcg acactgtcca cgatcttagt cgctctcgcg ctgaccgcga tgctcgccgg
     2881 ctcgtttgga gccccaccgt ctgcgaaggc cgctgacgac tcatgtcctg ccgtcgaggt
     2941 cgtattcgcc cgcggcacga atgaggcgca aggcgtcggc gcgaccggac aatcgtttgt
     3001 cgacgcactc accgcccgcc tgccgggcaa aacggtcgac gtatatgggg tgaactaccc
     3061 ggcatcgatg aacttcgggc aggccgccga cggcatcgtc gacgcgagca acaagatcca
     3121 gtcgatcgcg gcgagctgcc cgagcacaca gatcgtactt ggcggttact cgcagggcgc
     3181 ggcagtagcc ggttacacca cgaccgattc tgttccggcc ggattcgacc tgccggccgg
     3241 tatcaccggc cccatgcctc ctgcggtcgc ttcgcacgtc gcggcagtgg tcctgttcgg
     3301 aacgccagat gcctggatca tgaacctgct cgaccgcagc gcacccccca tcacgatcgg
     3361 ccagtcctac atcgcgaaaa ccctgcagct gtgtgcaacc ggcgacccgg tctgcttccc
     3421 cgggggtctt gaccgctccg cgcacagctc ctacaagaac aacgggatgg ccgaccaggc
     3481 tgcagccttc gcagtcggcc agttgaaggg tggcgcgccg gcagccacga tgcagcagac
     3541 ggcaggtgag gtcggtgcgg gccagcccgc cgctgagcga gcccctgcac ctgagcccgc
     3601 cgtcgacgca ccggttggca actgaatgac aacatcacca ccgaaccggt ggtgaccgac
     3661 caacggccgg aagtgagtcc caggaccact tccggccatt ggtctattgg gccgtcatga
     3721 acgatcgcac aatcaaggta tcgaacagca ggccaaggcc ggtggcggta ccgatcggcc
     3781 cgaggatgta cagaccgctg aagatgaacg aggacatggt ggccgcgaac accagacgcg
     3841 ccgccgccac gacaccgccg gtgccggcca tcgcgcggat gacaccggtc ttgagtccgg
     3901 ctccgatcac ttcctctgca tctcaagacc tttcaacggc tggtgtgccg cgagccgacg
     3961 actaggtctt gacgatgatc gtgccgattt ttccaggtcg gttgacgtgc cggaccgcgt
     4021 cgctgatctg atcgagcgtg tatgtgcccg caacgtcgaa ttctgcagat aggccgatgg
     4081 cgatggcgat gacgctagcg atatctgaag cacggcgttc ggctgccacg ccgctgagcc
     4141 aacggccgac ggtaagcccg cggatgccga tttcgccgcc gagtagggct gatgcgtgca
     4201 gagaaatggt gtcctgggca attgctccgt aggtgatcag cgttccgccg gaggacaaca
     4261 gggatatcag gtggcccgcg actgccccgc cgacggggtc tagtgcgacg gggatggggc
     4321 ggccttcggc gatggtgcgt atctgctgta cccaatcggg gttgtcggta gagaccacag
     4381 ccacgttcgg gtaccgggcg gcgagctcgc gggcacgatc ggcgctgcgc acgacactga
     4441 tcgtggcgat ctgatggtgg tcggcgctgg cggtgaaaag tcggcccacc gaagaggtgg
     4501 cggcgttgtt gatgatgatg ccgccgaatc cgatcgagaa gtgttgctgg gccgcgcggt
     4561 agagcaacag ggccgtcaac gggttgcaga tcatctgcgc ggcgacatcg tcgctgacag
     4621 agtccggcac ggcgacagcg acttccgtag cgacgttgat gcgttgcgcc caggatccgg
     4681 ggttggggaa gacggtcacg cgggtgccga ccgcaggggt ttcgacaccg gttcctgtcg
     4741 ccaccacgac accggtggcc tcgagaccgg ctaccgtgcg ctgcccggga tgtggagggc
     4801 cgaagccgac gccgacgagg tctccaggat ggatcgggaa ggcgcggact tcgacctgaa
     4861 cttgtccggg ccctggttcg gggggttcgg ggatttcggt aacttccagc acgtccgcgg
     4921 ggttaccggc ggcggcgtag gtgatagccc gtgccatcgt tggtcacgtc ctttatagct
     4981 agtcgggcgg tgatccgctt cagagctggt gtgaattgac acgtcacgtt aatcgatttt
     5041 cgggatctta cgtgatagat cacctatcgt gaaggcccaa gagcggccgg acacggattc
     5101 gggcggaacc gactcggtta cggcccactg agctgacaat cggaatgtgc gagaactggg
     5161 gtttgcggtg agtgatcgat ggttgatccg tggtacggag ttcacgaact gcaactgcgc
     5221 atggggctgc ccgtgccagt tcggcgcgaa gacaacccat ggacactgcg agacggtgat
     5281 gtgcggccac atcgaagagg gaaacttcaa cggcacgaat ctcgacggcc tgaactgggc
     5341 ggtgctcatg tattggccag gtgaggtcgc cgagtgcaac ggcacgcagc aggccatcat
     5401 cgacgagcgc gcggatcctc ctcagcgaga aggacttcgg aagatccttc acggcgactc
     5461 gactgctccc ggagcgacgc atttctacgt gtacaacagc acgatgtcga ccgtcctgga
     5521 cacgctgtat gcgcccatcg tgctgtcgat cgatgtcgat cagcgaatgg ccagcctcaa
     5581 gattgacgat ctagtcgaat cgcgcgggac gccgatcatc agtcctcaca ccggcgagcc
     5641 gaccaggtca cgcatcaaca tgccggatgg gatccactac atcttcgccg agatgggcaa
     5701 cggcaatacc aaagctcggg ccggcgtcga gctcgacctg aaggacacct acggacagtt
     5761 caacgttctg cacgtgaacc aagacgggat gattcgctga ccacgtcttg ggtctggcgc
     5821 catcgagatc ggttggccgt actggcaggt ctgggcagca tcacagtcgc cgcctggatt
     5881 tacgtcgtgg tggaagcgcg ccgaatggca agcgagtcgg cggacatggg gggtcggccg
     5941 atggcctcga tgatggagtc catgaccggg ttgcaaccgt ggaccgcaac cgaattcgct
     6001 ctgagactgg cgatgtgggc cgtcatgatg gtcgcgatga tggtgccgac cgcggtgcca
     6061 atgaccctgc tctacgccgc cgtcgcgcgg aaggcagctg cccagcacaa cccactggcg
     6121 tcgacgtcgg tcttcgtcag cggctacatc gcaatgtgga cgatcttcag tcttgtggcc
     6181 accatcgccc agcacgctct cgatcaggcg gcgctgctgt ctccgctgat ggtgacccgg
     6241 agtgcgggac tcggagccac gctgctgatt gctgccggcg tctatcagct gaccccgctc
     6301 aagaacgctt gccttcggaa ctgtcgagcg ccggcgcact tcctgtcccg ctattggcgt
     6361 actggggatc tcggcgcgtt ccgcatggga ctgaggcttg gcgcctactg cgtgggctgt
     6421 tgctggatcc tgatgggatt gctattcgtc ggcggcgtga tgaacctgct ttggatcgca
     6481 gcgatcgcca tcttcgtcct actggagaaa acgagtccat tcggtgatgt gagcggacgg
     6541 ttcgcgggcg tcgcgttgat cttggttggc gcagtgagtt tggctctgtg atgccaacgc
     6601 gctcaacccc gttcagccgg gtggcagtga cgagttggag ttgagactcg cctggatgtg
     6661 ttccagcgcc gctgtgaacg gcgaaagcag ctcgtcaggc accggatcga agaacaatct
     6721 cctgaccaac ctggcatggc ccggcgcggc cgccacgatg gcgctcatgc cttcctcggt
     6781 caacaccaca ttggtcgccc ttgcgtcgac ttcggactgg aggcgttcgg tgaggccgcg
     6841 gtcacacagt cgcctcagat ggtgcgacag acgactacgc tcccagcaga tgaccgcagc
     6901 caggtcactg atctgcagct tgcggttggg tgcaatgctc aacgcgcaga gcacgtgaaa
     6961 gtcggccagc gagagctcat tatctcgttg taactggcgg ttgatctcat agttcattcg
     7021 cagctgcaca cgtaagtagg cagcccttgt gcgttgctgt gacgacgaca accatgggcc
     7081 accgccgggg ttagaacagc gcataggtgc tttcacagtt cttctgctgg acttctcatg
     7141 gttattgtcg cttctcgact ccgagccgtc caggaccact ttcgactctg actagcccgt
     7201 tcacggctca gccgatgctt cgccagatcc taccggaata ctcagagctt tcaattgatt
     7261 gatacatcac gtaactctac taaggtggtg actatggcaa gagcagtgca attcgattcg
     7321 tacggcggcg tcgacgttct cgaggtgcgc gacgtgcccc gccctgcgcc gggcgccggc
     7381 gaggttctga tcgaagtcaa ggcagccggt atcaatatca gtgaggcggc gattcgagcg
     7441 ggcgccgtgc gcgacatgtt ccccgcgacg tttccatccg ggcagggcag cgacctggcg
     7501 ggcgtggtgg ccgaactcgg ctccggcgtc aagagtttcg ccgtaggcga tgaggtgatc
     7561 ggcttctcca atctgcggtc cagccacgcc gagtacgtgg ccgtgcccgc gacccagttg
     7621 acgccgaagc cggccgcggt gccgtgggag gtggccggct cgctgttcgt cgcgggcgct
     7681 accgcgtacg gcgcggttcg ggccgtgcgc ctcgcaccag gcgagacggt ggcgatcgct
     7741 ggtgcggcgg gtggggtcgg atcgatcgcg attcaactcg cccgacgcgc cggtgccacg
     7801 gtcctcggca tcgccgggcc gtccaacgat gcctggctca ccgaccatgg tgcggtcccg
     7861 gtcaactacg gtgtcgacct gccggcccga ctgcgtgccg cggcaccgtc cggacgcatc
     7921 gacgccctgc tggacttctt cggtgatgga tatgtcgcga tggcggtcga ggacctcaga
     7981 gttccgcgcg aaagggtcga taccatcgcc gatttcgctg ccgtcgagcg gttcggggtg
     8041 ctgtccgcag gaggtgcgga tgccgcgacc gcagcggtgg tcgccgagtt ggccgacctc
     8101 atcgcacaag gtcagctgga tgttccgatc gcaggagttt tcgccctcga cgatgtgcgc
     8161 aatgcgtacc gggagctcga aaagcgccac acccgcggca aattggtgct gcgaccatga
     8221 atgatatgag gagaccacaa tgagtgatgg caacgacttc aacgagcgga atatcgccga
     8281 gttccgcgcc aaccacggcc gcgtcagcgg cccgttcgaa ggtgcgccaa tcgtgcttct
     8341 gcacaccgtc ggcgcccgca gcgggaaacc gcggaccagc atcatgatgt acctcgccga
     8401 cggcgaccga tatcttgtgt tcgcctcgag cgccggcgcc gaccagaacc ccgcctggta
     8461 ctggaacctg aaggccaacc cccacgtccg catcgagatc ggcgacgaca tcgtcgacgt
     8521 gcatgccacc gagctgcagg gcgccgaacg ggacgagaag tacgcgatac aggccgagcg
     8581 gtatcccggt ttcgctgagt acgaacgtat gacgtctcgg acgatcccag tcatcgcgct
     8641 cactccaacc ggtggggcgc gctcatgact gagacaggta gttcctcgca agtatccggc
     8701 gtgccgcgca tggacttcga tgccctttac cgtggcgaaa gcccaggcga aggcatggca
     8761 gcgatgacca cgccgccgtg ggataccaag gagcccaacg agaatgtcat cgcgtggcag
     8821 accggcggct gggtccacgg cgacgtgctc gacgtcggct gtggccttgg cgacaacgcc
     8881 gtctacctgg ccaagaacgg tcacgcggtg accggattgg acatctcccc cactgcgctg
     8941 atcaccgccg agcagcgggc caaggacgcc ggagtcgatg tgacgttcgc ggtggccgac
     9001 tccaccaagc tcgacggcta cactgacgcg ttcgacaccg tcatcgacag cggcttgttc
     9061 cacagcctcg atgacgacgg ccgacgtagt tatgccgcag ccgtgcaccg ggccacccgg
     9121 cccggcgcca ccctgttgct cagctgcttc tccgacgcca acctggtggg ccaggagtgg
     9181 ccgcgaccgg cggtgtccga agagacgctg cgcgatgtcc tcggcggcgc cggctgggac
     9241 atcgtgtctc tggaggccgc cacggtgcgc ggcgcactgg atggcggccc gcaggctgaa
     9301 atggcgttct ggtacctccg cgcgcaacga cgccaactcg tttgatactt ggcctgtcgg
     9361 ttcacacgtg atcggatcga gcaagttagt ggccgcgacg taagtggcta cccggtgacc
     9421 agcgcgcggg tctcggtcca atcgaccatc agcttgcgtt ggctgaagta ccagatgcca
     9481 tcttgtttga cgaacgtatc gaggtagcgg atcgcggcaa tcatcagact gcgccctgcg
     9541 gcctcgacct tgacgtggtg agccaagcag taggcgatgc ccgtggcgtt ctgcccgtcg
     9601 agcatcgtgg tgctttgacc attgaaatgc atcgtggcct cgtaaccgtt cagctcgtcg
     9661 aacaccggag cgagcgctgt acgtccctga atgtgttggg ccggtatcga actgctggtg
     9721 tccatgtaca cctgaaaatc ggtgtcttcg gtgaacagcg ccatctgacc agtggcatct
     9781 cgccgatcgg cgcagaaggc gtaggtgtca accaattctc ggatcgccac ccggtcggcc
     9841 atctcttgaa gcgaaagatc cacatgcgca gcgcgccgga aatccccgga tgtcatagct
     9901 atcctccccg tccagaagaa cgccgaccga ttgacacacc atgcgactct acttactgtg
     9961 tgatattaaa tgataagtca tgcagatgaa agatctgcag gacggaactc catattgttc
    10021 accggcttcg tcgcggagct taaattgata actcattcaa tgaagggtgt ctccgatcag
    10081 cgccacgatc agcgaattga tgcacgccaa ccttctcgag gtcttcaacg agcgtgatcg
    10141 tgtacgtcga tctgctgcga tcgcgcggat ctacgccgat gacatcagat ggaccgacga
    10201 cgacggtgtg accatcgggc acgcggcgtt ggacggcaaa gccgcagagc tgcagtcgaa
    10261 gcttggcgat ctacagttca ttgcagcggg gcccacgtat caaacattgg gcctgggcta
    10321 cctggcattc aatgtggtca aacccgacgg cggcgccccg cagatctcgg gtttcgacgt
    10381 tgcgatcgtt cgagacggac tcatcaccga gctctacacg gtgctgaccg acctgccgcg
    10441 atgaaactga cgggaaccct gccgaagacg caactactca aaggagattg agcaatgacc
    10501 acagcctcca acctgacctc gccggccgac attgtcgtcg ttggcggcag ctccgcggtg
    10561 ccaccccggc aggcgggtag gcagcgggtc ttccatctag ttgatacgtc acgtaatgtc
    10621 tcatcattaa ggaagggccg tcatgacaga gttgacgatt gcgatcatcg cgggcagcac
    10681 ccgcccggga cgccacgccg gcgcggtctc cgagtggata ctcaagcagg cggcggaacg
    10741 cctgggtgtc cgctacgaca tcgtcgacct catagaccat cccctgccgc tgcttgacga
    10801 agccatgccg cccagcttcg ggcaatacca gaacgaacac accaaggctt gggcggcgac
    10861 gatcgcgcct tacgacggat tcgtcttcgt cagccccgag tacaaccact cgacgtccgc
    10921 ggcgctcaag aatgccctcg actatctata tgccgagtgg cagaacaaag ctgccgcatt
    10981 cgctgcttac gggtcgctgt ctggggcacg cgccgttgag cacttgcgtt tgatctgctc
    11041 cgagttgcag atggcaaccg ttcgccaaca actttctttc tcgttgttca ccgacttcga
    11101 gaacttcacc gccttcactc cggcggcatt gcacaacgac tcggccgccg tcatgttcga
    11161 tcaactcgaa agttgggcag gtgcgttgaa gacactgcgt tcctgaacac cctcgctacc
    11221 gagggccgcg cgcgacatca ctggcgccga ggacgcaaca cgtgcggagg acgacaacaa
    11281 tgagcacact gaccggtgac cgctaccgcc tcggagacaa ggtggtgcac cgcgtcgggt
    11341 acggcgcgat gcaactggct ggtgatggtg tcttcggtcc accccgggat cgcgacgaag
    11401 cgatcgcagt gctgcgcgcc gctgtcgatg ccggtgtcga tcacattgac accgcccagt
    11461 attacgggcc cggggtggtc aaccagctga tccgggaagc actctctccc taccccgaca
    11521 ggctggctct ggtaagcaag gttgccgcgc gccgagacga ctttggcgcg gtcctgcact
    11581 acgacgaccc cgaccaactc cgtgccggca tcgaagacaa cctcgcgacc ttaggtgtcg
    11641 agcagctcac cgcagtcaat ctgcgcgtga tggacaacgc tgtgcctgat gaaaggtttg
    11701 tcgagcagat cggagcgttg gtagctgcgc gtgacgaggg cttaatcgcc ggcatcggca
    11761 tcagcaacgt gacacaccga cacctgcggc gagcggttga agtcaccgag attgtgtgcg
    11821 tgcagaacgc gtacaacctt gcggatcgat cctcggagcc cgtcctcaag gagtgcacca
    11881 cccgaggcat cgccttcgtt ccgttttgcc cgctgggctg gccgaagagc caacacgatg
    11941 cgatccgcac caacccgatc gtcaccggca tcgctgcagg ccacggcgct acgcctgccc
    12001 aggtcgctct ggcctggcta ctggccgttg ccgagaatgt gttgctgatc ccggggacca
    12061 gcacccgcgc acacctcgca gaaaaccttg ctgcggggtc attgtcgttg cgcgacgacg
    12121 aaatctccag tctcagctcg gcattcggcg tgcggtgagt tgtcttcggc taccaccgcg
    12181 gcgatgccgc cagaccgtcg tatacacggc gcaagaccac tcgccggtac caatcggcca
    12241 ttcccgtcct agccgactcg caggtattca cccgtcagtc tcgtcgaggc aacctgctct
    12301 ggcggctgac gcttccgtcg ccggagtcgc tcgcatacca ccgtcgatcg gcggatgact
    12361 ggcctgaaga ttagggaggc tctcgccatg aagacaatgc ccgccgtggt gttaaccgct
    12421 gaacgcgaaa ttgccctcga ggaacggccg cgcccatcgt tgcgatctga tcaggtcatc
    12481 gtcgaagtag agctgtgcgg gatctgcggg agcgatctgc actccgctca gatgccgcag
    12541 gtttatcacg gagactgcac tctggggcac gaatctacgg gccacatagt cgctgttgga
    12601 gccgacgtcg ccgaatgggc tgtcggtcaa cgcgttgcga tcaaccccaa tggaaacgtc
    12661 tgcgggactt gcaaattctg cagggcgggc caatccaact tctgcgttca agccaccctt
    12721 gagacggcac tggggctaca ggcggacggc ggactcgcgc cgttgatggc ggcgtttccg
    12781 tcccacctcc gagccatccc gcgtggactc gcgtcgcgcg aagctgcatg ggttgaaccc
    12841 gctgcgacag ccttacgcgc tgtggaacgc gccggcgatc tccagggacg cactgtattg
    12901 gtcactggtg gcggtccgat cggtcaactg gcgtgccgat tggttaatct cgaatcacca
    12961 gagcgcgtgc tgctaatgga gccagctgtt gatcgggccg agttcgcgag caattcgcat
    13021 gccaccgtca tcgctgccga cgaggcgacc gagtccgaca ctagggtcga tatcgttatc
    13081 gagtgctcgg gcaacgagac tgctaccgcg tcggcactga gcctgctcac ccctggcggc
    13141 actctcgtcg tggtcggagg agggcgcggt tccggactcg accccacaac catattgctc
    13201 aaggagatca cagttcacgg gtcctacacg tacatcggcg agttcgaccg tgccatcgat
    13261 ctgcttgcga cgggccagct cgccgtcgca gacctcacca gtgttgtgac accactgtcg
    13321 gacaccttga gcgcgttcga ttcactccgc gccggccgaa tcatgaaagc gctcatcgca
    13381 ccaagccagc actgatgggc acccaccctc aacagagagc aaacatgacc gacacaccac
    13441 cgaacaatgt cgatctggtg cggaccttga tacatgccgt cgacaatgcc gacaacgaca
    13501 ccatcgccgc ctgcacggcg aaagacgtcc acttccgatt cggaaatgcg caaccgacca
    13561 ccacgcagtc ggagctactt gcggcagccg gttcttttcg tggcgggatc gccgctctgc
    13621 ggcacaccat cctcaacatg tgggaggtcg acggtgggac cgtgatcgcc acaatggacg
    13681 tgtactaccg ccgactggac ggtggcgagc ttaatctgcc gtgctgcaac atctttcggg
    13741 tagaagacgg tcgggtagtc gactacctca tatacatgga cgtcaacccg gtaatcgcga
    13801 cgtagcggtc tgtggagcgt gcggacgtac gcgttcgccg cgaacgccaa cattcagccc
    13861 cgggcttcgc cagatttcca atgacgacgt ggccgtcgtc gccatacccc tatcagatcg
    13921 cttggcgcgt cctcagagag gtactggagg gagcgatgca ttgaggttga gacttgcctg
    13981 aatatgttcc agcgcagcgg taaaaggatt aacaaggtcg tccggtaagg ggtcgaagaa
    14041 caattttcta accaacgcaa catgttccgg cgcggcggcg actatgacct caagtccctt
    14101 cttggtcaag agtgcatctg tggccctgcc atcgtcttcg gactggatgc gctcggtgag
    14161 tccgcgttcg catagtcgcc gcacttggtg cgacaaccgg ctgcgttccc atccgatcag
    14221 agcggcgagc tcggtaatct gtagcttatg gtccggcgcc atactcagct ggttaagcac
    14281 gtggtagtcg gccagtgaga gcccgctgtc tcgctgcagc tgacgattca tctcatagtt
    14341 catccgcaat tgcacgcgca tgaacgccac ccacgcgcgc ttctgggaag cgggcaacca
    14401 aggattgtca cctgaacggg agcggcgggc gggcgacatt tcacgatcct tctgctggac
    14461 tcttcacgct tattatccac cacaccggtc tcaatactga tggtggtcgt tattgcgtcg
    14521 acgtcggcct ctcccccagc gcgcggggca tcggtcaggg cgaccagaat cgcaaccgtg
    14581 cgagtgtcgc cggccaacag ccacaccggc gttctggttt cgcgttcgga gtagtacgcc
    14641 cccaacccgc cccccgccac tgacgcggta tgcggcgggt gacgtattcg ctcctagtct
    14701 cttgatttca gcgccgccag taccaccacg acgcaggcca cgcgacgcag gccgccacga
    14761 ccgcgaggcc gagagaaact cccacctgaa gggccagcaa aaggactgaa cccatcaccg
    14821 ccgggtaggt cagcacggtg tcaacgtcgc gcttcacgcg gcgctcgcct tcaggccaag
    14881 cggcccagcc agttcggtca gcgtcggcaa cagtttgtcc gagccaccga gcacttggat
    14941 cacgacttgg ttggcgccgg cccgcagatg ctcacccagg cggttggcga tgtcgccggg
    15001 ggtgccgtag gcgaccaggg cgtcgatgaa ctcgtcgctg ccgggcgggg tgaggtcggc
    15061 gtcggtaaaa cccagccgct tgaagttgtt caggtaattc gtgaggttga ggtagttgta
    15121 cgtgttcaga accttgcggc cgactgcgcg cgcctcctcg gcgtcggcga tcggcagcac
    15181 tttgtgctcg ggtagcagca gtgcgttcgg cccgatgatt tcgcgggcct gtgcggtgtg
    15241 ttcgggtgtc gtgttgtacg ggtgcgcccc ggcggtgcgt ccagcggcca gcctcagcac
    15301 tcgcggcccg agcgcggcga gcacacgctg attggccggt acgccatatt cgtcgagctc
    15361 gtcgaggtag tcgaccagcg cgtcgtaggg cttgcgatat tgctcagtat gctcgggatg
    15421 gccggccccg atgccgagca cgaatcggcc ggggtgggcg gtattgatgc ggtggaacga
    15481 ctcggcgacg gttttggcgt ccgccgtcca gatgttgacg atgccggtgg caacctttaa
    15541 cgcggtggtg gactccaaaa gcggctcggc gaacgacaat tcggcggccg gcgaaccagt
    15601 aacccatagc gtgccgtagc cgagccgttc gatttcctgg gcctgctcgg gggtgactgc
    15661 cagcccgagg tcgaggaaac tgctgcctgg gtcccaggta aacacgccga atcggccaag
    15721 gtcgatgttt ggtgttgtca tcggtgtgct cgcagtatca atgcctgctg aacccggtcg
    15781 ggcgaaccac gatgaccacc cagtccgggg cgtcggaggg agcctcgccc cggggcgggg
    15841 cgtcgggggg aggttcgtcg ccgctgtaac gcttggcgat acgccgaaag aagctcgccg
    15901 ctgggtcggg ctcgatgcgc tcgacgtcgc cgcgcacttc cagatagcgg tacgggttgt
    15961 cgggatccga gatcgacatg gcaacccgcg ggttgctctg tacattgcgg tacttctgcc
    16021 gcttggtggt gtgcgcgaat cgcagcagct cgccgtccca gtcgaaccac atcggactca
    16081 cttgcaccga accgtcgggc cgggccgtcg ccaaatgccc gtacagcggg agttcaagca
    16141 gatcgttgta gccggcggga accgcaacca tatttgctcc tatctagatt tgttctgtgc
    16201 cgacgattcc ggggaaatcg tcgagcgacg tgtttggacg aaagccgttt caccgcaggg
    16261 gtactcgagg tggagaggct ccgcgtgatt agacctgtgc catgccacca tcgacgctca
    16321 gttcgacgcc ggccacgaag ctgctgtcat cggaggccaa aaacagtgca gcggatgcga
    16381 tctcgtcggg gcggcccaac cggttgagtg gtaccgccgc cacgaactgt gccttgtcgg
    16441 cctgaccgtc caacatggga gtgtcgatcg ggcccgggct caacgtgttg aaccggatgc
    16501 cgcggtcctt gaactcccgg gtccaagtgc gggtatagga ccgcagcgcg gccttggtgg
    16561 cgctgtaggc gctgtaaccg gaaacgccca tgtggcctga ggtcgagcca atcagaatga
    16621 ccgagccgcc atttcgaatc catggcagcg ccttttgcac cgtgaacagc gttgcgcgag
    16681 cgttgacgtt gaaggtggca tcgaagttct cctcggtgat ctttcccagc atttcggtct
    16741 ccgcccgggc cgaattcgcc accaggatgt ccagcccgcc ctctgcgttg aggaccgtca
    16801 ggaacagcct gtccaggtcg gcgttgctgg tgacgtcccc cgcgacggtg ctcaggccgt
    16861 cgccgatcag tgctttggcc ttgtccagct cggtgcggcg gcggcccatg atgaagacgc
    16921 gggcgccttc gtcgacgaac ctctgtgcgg tcgcaagccc gataccgctg ctgccgccgg
    16981 tgatcacggc gaccttgcct gccagtcgtg ccatcagttc atcctccaat ttcgtgttgt
    17041 gtgctgaacg tgccgatacg gtgtctgttt ctccgataac cgtcgttgcg cggaaatggg
    17101 tgcggtgcga gcacattccg tgtcgcttct gttgggagaa catcgcgaga ggtcatggcc
    17161 gcagaccctt gatcggtgtg cccgatatgc ccgaatcgtt ggaaccgact gactgcagga
    17221 tccgttaggg catgttggac gcattgtgga tacttaggtg ccgcacgacg gcgtccgaga
    17281 agtccggaac actgttgccg ccctcgcgaa ggtcttcgag gaattgctgg tagaggtgcg
    17341 caaccccaaa tgcgccttgc cggtagaagt ccgcaacccc aaggccgccg ccggtagcag
    17401 gccagtccag tgatgcgacc ttgcggtagg actgggggac gggcagcaca gccagctcgg
    17461 cggcgtcgcc ccgggcgccg agcaatgtca gcggggcgag ttgtatatgc ccgattgggg
    17521 ccgtgacaac gatgtctccc tcggtaccgt tggataacac ctcaccgacg tatccatcgg
    17581 ccacgaggtc ccgcaagtag ttcatcaccg gattggtgcg aatctgcaga ccgacgatgg
    17641 atggcacatc gtgttcgccg acgagacgag caagctcttc agtctcagcg agaccattac
    17701 ccgggggcca ctcgcagaag acggccctac cggcttgaac cgctgcgcta accaactcgc
    17761 ggtgtgccgg caccctgacc gacacgacaa ccaaatccac ctcatcacag gcagccagat
    17821 cggttgcatt gccgaaggtc cgcgccaccc cgtgtttctc accggcgctg gctgccgatt
    17881 ccggtgagct ggtactgaga gccgtcacct caaagcccgg gattgcccgc agcgccggca
    17941 ggtgggcgag gcacgcccag aggtcttctc tagcaccgag accgataatc cccacgcaga
    18001 gcggctgtcc ggaccgtgcc catctcgcca ctacagattc tgctgttgtt gtcatgtgtt
    18061 tctcctgtgt caagccgctg ccgattgcca aggctggttc gggcttggtg gacaactacg
    18121 ccacccacaa gacccccgag acccacaacc ggctgccccg gcatcgccgc ttccacctgc
    18181 acttcacttc ccccagctcg tcgtggctga accgcgccgg gcacgccggt gccaccgttg
    18241 ccgccgttcc tgccagtcgc accatcagtt gatcctccaa tttcgtgtgt gcgccggact
    18301 gtgccaggtc agtagtcggc gggctggttg aagacgatcg cgaggctgac gatctggccg
    18361 tcgcgcacgc tgaagtagtt cgtcagaaca atttcgtcgg gcagattggt ccggtcgaac
    18421 gtgccgtcgt aggccccgcg caccacggtg tcgccgtaat ggtcgagcac ctcgcgcact
    18481 tcgatcgtca ccttgtcgcc gaccatctcc ctggccaccc agcggcggat ggcgtcgatt
    18541 ccgacgaatt cgcggtggtt gtcgttgacg taggcatctt cggcgaacgt ggcgaccgcg
    18601 cgatcggtgt cgccggcgtt gatcgcgtcg acgtgttcgg cgacgatccc cgtcaggtcg
    18661 atttctttca tgctcttgtc tcccactccg tggccggcgc ggtgttgcag ccggtgatcc
    18721 caccgtgggc gctcacaccg tgggcgagtc aaggcctgtt caggacgtga gtctcccccg
    18781 gggggagagt gcacactgag gccatgagtt cacccgcgct cttggccatc ggcgacttct
    18841 cccgtgcgac gcagttgagc gtcaagatgc tgcgccacta ccaccaggtc gggctgctcg
    18901 agccggttga tgttgatcgc gctaccggct accgccgcta cagcatcgac cagatctcga
    18961 cggcacagat cattcgccgc ttccgggctc tggaaatgcc gctcgacgac atccgcagcg
    19021 ttatgagcac caccgacatt cgtcgacgca acgaactgat caacgcgcac ctcatgcgcc
    19081 tggaatccca actcgcgcag accaagcggg ccgtcgagtc gcttcgtgaa ctgcttgagc
    19141 ccggccctgt tgctgaaggc cggataggtt accgcagagt cgaagcgacc gaggcaatcg
    19201 cgatcagcga ggtcgtcgac ataagcgatg cgctgacctg ggtcccgggg gcgctggccg
    19261 agttgtatgc caccctgact gctcaaggcg tgcgcatctg cggcccggct gggggcatct
    19321 tcgccgacga gctagtcgcc agcgaacgag ggcaggcgac cgttttcgtc cccttcggcg
    19381 gtgacttccg acgcaccgga cgcctaaccg aactcgttgt accagccgcc gaattggcca
    19441 cagtcgtgca cgccggctcg tatcgcgact tcgacctggc ctacggcgcc ctggccaccc
    19501 acgtcgccca acacgcgatc gccgtcgacg gcccgattcg cgagtacttc ctcgtcggcc
    19561 cgcacgacac ctccgacgaa acggcctggc gcaccgagat tggctggccg gtctttcaca
    19621 ccacaaccgg ctaaccccgc ccacgcgccg tcatcttgca tgcccaccga cagggcaacc
    19681 cgcccacatc cagaggtcca cgctcgactg ggctcgagta aggaccctta ggtgtccgaa
    19741 aggtggagct ttgggaactc gaactcctgg cccccacact gccaggacca cgcagagcat
    19801 gcgaacaggc cgtttattag ccacagagga ctgtcgtggg cgtaggcccg cgcgcgaccg
    19861 tcttcagcgt tgtcgttaga aatgtggtcc gccgtcgttc acaaatccgt tcacggtagt
    19921 cgtgaagcgc cgcgacaact gccacgctgg ctcgccgtgc tgttgggtcg ggtgtcggct
    19981 gtttcggcta agcggctcgc acgtgacgtc cgacgatgtc gttgatcctg ctgacgctgt
    20041 tcgagactcg tcttcaaaaa gaccagaatc agcggggatt catcagctgc gagctgaaga
    20101 cccacacggt ccccatgcag cgacgtcgaa ctctcgatgc tggcggggcg cttctactcc
    20161 cgccgagtct ggtccagttc agagacccct tcaactgctc aacctcggcg gagcggacgc
    20221 tctcctaaag ccggtgtcgc aggttcgaat cctggcgggg gcacttcaga gggcattttt
    20281 gtttacgccc cacgaaaatc catcgtttat tcatcgctta tggtccggtc cagtagctcg
    20341 gcgacggcgg tgtgggaccg gcccctggcc atgtaggtgt cctgagtctc ggacacccgt
    20401 gcgtggccta ggtggtcggc tccgattcgg gccgacagcc cctcgtcgtc gatcagcgtg
    20461 gcaacggttt tcctgaagct gtgcgatgtc acgtccggca cgcccaactc ctcgcggacc
    20521 tcccgccacc gagcgcggaa gttgtcgggg tcgcgccatg tgcccgccgt cgacgggaag
    20581 atcatcggct gctcaccgag atacgcgcga ccccgtcgag cggccagcac tgcgaccgcg
    20641 aacgtcggca gcggcagggt ccgagtaccc gcggcggtct tcgtctcgtc gacacgcatc
    20701 agccccttgc ccgcggccct gatcaccttg ccggtaatcg tcagcgtcgg cgccgtgtcg
    20761 tcgaactcgg tccaatacag cccaagcagc tcggaaatcc gcagccccgt cgcgatgaac
    20821 aacgtgatcg ggtccacgag gtcgtgacgc tgacacgcct ccgatgcccg cagcttcccc
    20881 agcagtgcgc gcagctcgcc agcagtcagc gccggtgcac ccttcgggcg cgacgtcgag
    20941 cgcatgggcg acacgtcgcg caccgggttc gctccgatga cgttcgccat caccgccaag
    21001 tgcaggccgc ccttgaggat cgtcttggac tgcaccgcca tcacgtcgcc gtgggcccgg
    21061 cgcatcgagc ggatggccgc gtcgattctg gccggcgtcg cctccccact cgaaccccgg
    21121 cgatgatctt gctcaacaac ttcgcgcagt accggtacgt gtcgatcgtc cgcacggccc
    21181 ggccatcctc ttcgagacga tcgatgtgtc gatcgactag agccatgatc ttcgtgtcga
    21241 gcgtgacgcc gccgtcgccg gtggcgcgac gttgcatgat cgcctcgacc aacgcatcct
    21301 cggcgagctt gcggtgctga tcgtgttcat cagccgggcc gcgccgctcg acgatcctgg
    21361 tcactccgtc agcgtcgcgg aatcggcagc gcgccaacca cactccgtcg ccgagatact
    21421 tccggctgca cggctcctca tcggtgtaac cccaagtagg tgcgcgcagg gggtcgaaag
    21481 agcttgcccg aagggataga gtgaactttg accccgagag tggcccgcga tgggcccggt
    21541 gggaggaccg ggttgtcatt tttggctaac gagtggggtt ttgctagcct aactatgaac
    21601 ggacatggta tgcaccggct actgaggggg cttgccgtat tagcagctct gccactcatt
    21661 gctctaccga ccgcagaggc cgccacggta ctgatgatca gtccactgac aacgaacatg
    21721 caagacgagc ttcaaggctc tatgtgcgag accaacacct gtgtcgtggt cgatgccccc
    21781 gcctaccccg atccgcgcgg cgtgatcgcc cttgacaatg ccattaagtc cacgcctggc
    21841 gaaataatcg tgtttggcta cagcaacgga gcgcaaatag tctcacagtg gctgacgcag
    21901 cacgccaacg acccgaccgc tcccagcccg gaccgcctgt cattcgtgct gatcggcaat
    21961 cccacccgtg cctacggcgg tacgtcggcg ccgatgccgc aaaccaaata cctcgttacg
    22021 gacatcgccc gcgagtatga tgtggccgcc gattacccta acaatccgaa gagtcgctac
    22081 tactcgctgg ccgtcaacaa tgcttgggca ggtttcttct caatccacgc gaactactgg
    22141 gacgtcaatc agtacgaccc cg
//
LOCUS       DASRJA010000463         3561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3056393,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000463 DASRJA010000000
VERSION     DASRJA010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3056393"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VFC01_20430"
     CDS             <1..1188
                     /locus_tag="VFC01_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN82027.1"
                     /translation="QPPYKTAGAVLLVLVIAVAAFIYLQFRGDLTKKTHLTLLSPRAG
                     LVVEPGSKVTYNGVEIGRVSRISMVDENGTPKAKLTLDVNPDYFKYIPANVAADIRAT
                     TVFGNKYISFSSPKNPSPQHISSHDVIDASAVTTEFNTLFETVTSIAEQVDPIKLNQT
                     LAATAEALSGLGDRFGQSLDNGNRILDDLNPQMPQIAEDNRLVADLADVYADASPDLW
                     DGLDNAMTSARTFNEHSADIDEALMAAVGFADDAADSFERGGPYLVRGATDLVPTTQL
                     LDDYRGMILCTIRKFSVVQKRASAALGGNGYSLASSSGTLAGAGNPYVYPDNLPRVNA
                     RGGPEGRPGCWQDISRELWPAPYLVMDTGASIAPYNHLEIAQPVLIDYVWGRQVGELS
                     INP"
     gene            1229..1651
                     /locus_tag="VFC01_20435"
     CDS             1229..1651
                     /locus_tag="VFC01_20435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZN82028.1"
                     /translation="MTFKSYELDFMRQADLGRLATIQPDGTPQNSPVGFTYNEELGTI
                     DIAGFRMSKSQKFRNVAHNAKVAFVVDDITSRDPWRVRCLEIRGTAEQAEIPPSHGAA
                     GDELDTAIIRVTPRRIISFGIDDQVSEPHQLTVDIRDV"
     gene            1677..2945
                     /locus_tag="VFC01_20440"
     CDS             1677..2945
                     /locus_tag="VFC01_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82029.1"
                     /translation="MPKKMVAGQGEAVGGLGMTALPTWQATGLSLAVIVGHVFNGSGA
                     LVFGLLSVGVIWTLHRLHAQAPRPRTTADLIASTPGAAPAQAIVVIQFVAYVLLGAYT
                     AKSIASMALVWMTNPDTTMPDWWGPALAVAAAAVGTVLVAALPTRLLAPTVTVLAAFG
                     LLVFFYIALAVIARVLSGTAPVEPAMDFGTTPAAAEWGPAAILISLAIAFAGFEIPTA
                     VSDRLRSVRRPLGGAMALVALSAALAWVATNLGSTGEFRYDAADLVIIATQMFGDTGN
                     KWLIAATIAQAVAALLVLIWGATRAVRPPVANSPLPLAMTAVVMALVALAISSDLWHA
                     APRLWGVAGILLLVVYVAAAQANSRLDDSNTTAWAVFALMGLVVVVVVFVTGVSDGWW
                     SAGIAAAITAAAAVWARAASHPGRPALRPR"
     gene            complement(2875..3507)
                     /locus_tag="VFC01_20445"
     CDS             complement(2875..3507)
                     /locus_tag="VFC01_20445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZN82030.1"
                     /translation="MQPSRFRAVGLAAALVGWSFVAPRVRWHPIPNASLGTGLVAITQ
                     SPLGLRPPALRSGLRYGLAAGAVAALGVAATAALPPVRRAMEKRDLPEHLVRWLVLDI
                     PIGTVWPEEAAYRATLGTVAEAAFGPTRGRLLQAAAFGLWHIVDARVTGQPVLGTVLL
                     TGVGGWLFGWLHARSGSLAAPMLAHLAVNEAAALAALGVKRRAPTPLRPP"
BASE COUNT          581 a         1210 c         1174 g          596 t
ORIGIN      
        1 cagccgccct acaagaccgc cggcgcggtg ttgctggtgc tggtaatagc ggtcgcagcc
       61 ttcatctacc tgcaatttcg cggcgatctc acgaagaaga ctcatctgac cctgctgtcg
      121 ccgcgggccg gcctggtggt cgaacccggg tcgaaggtga cctacaacgg cgtggagatc
      181 ggccgcgtct cccgcatctc catggtcgac gaaaacggca cgccgaaagc caaactgacc
      241 ctggatgtga atccggacta cttcaagtac atccccgcca acgtcgctgc cgacatccgg
      301 gccaccacgg ttttcggtaa caagtacata tcgttcagct caccgaaaaa cccatcgcca
      361 caacatattt catcccacga cgtgatcgac gcgtcggcgg tgaccacgga gttcaacacc
      421 ttgttcgaaa cggtcacttc gatcgccgag caggtggatc ccatcaagct gaaccagacg
      481 ctggccgcga ccgccgaagc gctgagcggg ctgggcgacc ggttcggcca gtcgctggat
      541 aatggcaacc ggatcctcga tgacctgaac cctcagatgc cgcagatcgc ggaggacaat
      601 cgcctggtcg ccgacttggc cgacgtctac gccgacgcat cgccagacct gtgggatgga
      661 ctggacaacg cgatgacgtc cgcgcggacc ttcaacgagc acagcgccga catcgacgag
      721 gcgttgatgg ccgcggtcgg cttcgccgac gacgccgcgg acagcttcga gcgcggtggg
      781 ccgtatctgg ttcgcggtgc caccgacctg gtcccgacca cccaactcct cgacgactac
      841 cgcggcatga tcttgtgcac gatccgcaag ttttccgtcg tgcagaagcg ggcctccgca
      901 gcactgggcg gcaacggcta ctcgttggct tcgtcatcgg gcacactcgc gggcgcgggc
      961 aacccctacg tctaccccga caacttgcca cgggtgaacg cgcgcggcgg ccccgagggc
     1021 cgccccggct gctggcagga catcagccgt gagctttggc cagcgccgta tctggtgatg
     1081 gacaccggcg cgtccatcgc gccctataac cacctggaga tcgcccagcc ggtgctgatc
     1141 gactacgtgt ggggccggca ggtcggcgag ttaagcatca acccgtgacc gttgacatca
     1201 acttttgttg aggttctacg ttggggccat gaccttcaaa tcgtacgaac tcgacttcat
     1261 gcgccaggcc gaccttggcc ggttggccac catccagccc gacgggacac cgcagaacag
     1321 cccggtggga ttcacctaca acgaggagct gggcaccatc gatatcgccg gcttccggat
     1381 gtcgaagagc cagaagttcc gcaacgtcgc gcacaacgcc aaggtggcgt tcgttgtcga
     1441 cgacatcacc tcccgggacc cgtggcgggt gcgctgcctg gagatccgcg gcaccgcgga
     1501 acaggccgag atcccgccct cgcacggagc ggccggcgat gagctcgaca ccgcgatcat
     1561 ccgcgtcacg ccgcggcgca tcatcagctt cggcatcgat gatcaggtca gcgaaccgca
     1621 tcagctgacg gtggatattc gggacgtgta gccccggccg ctgcacactg cttgtcatgc
     1681 caaagaaaat ggtggccggc cagggggagg ccgtcggggg gctcggcatg accgccctgc
     1741 cgacgtggca ggcgaccggg ttgtcgcttg cggtgatcgt cggccacgtg ttcaacgggt
     1801 cgggcgcact ggtgttcggc ctgttgtcgg tcggggtgat ctggacgttg caccgcctgc
     1861 atgcacaggc ccctcggccg cggacgacgg cggacctgat cgcgtcgacg cccggtgcgg
     1921 cgcccgcgca ggcaattgtg gtcattcagt tcgtcgcgta cgtgctgctc ggcgcgtaca
     1981 ccgccaagag catcgcatcg atggcgctgg tctggatgac caacccggac acgacgatgc
     2041 ctgactggtg gggtcccgcc ctggcggtgg ccgccgccgc agtggggacc gtactggtgg
     2101 ccgcgctgcc gaccagactt ctggcaccaa cggtgacggt gctggcggcg ttcggcctgc
     2161 tggtgttctt ctacatcgcg ttggccgtga tcgcccgggt gctttcaggc actgcgcccg
     2221 tcgagcccgc catggacttc ggcacaacgc ccgccgcggc cgagtggggc cctgcagcaa
     2281 ttctgatttc actcgccatt gccttcgcgg gattcgagat cccgacggcc gtcagtgacc
     2341 ggcttcggtc cgtccgtcgg ccactcggag gcgcgatggc gctcgtggcg ttgtccgccg
     2401 cgctggcctg ggtggccacc aatctcggct ccacaggcga attccgttac gacgcagccg
     2461 acctcgtcat catcgcgaca cagatgttcg gcgataccgg caacaaatgg ttgatcgccg
     2521 ccacgatcgc gcaggcggtc gccgccctgc tcgtgttgat ctggggcgcg acgcgcgccg
     2581 tccgcccgcc cgtagcgaac agcccgctgc cgttggcgat gacggcggtc gtgatggcgt
     2641 tggtggcgct ggcgatatcg tccgacttgt ggcatgccgc cccaagactg tggggtgtcg
     2701 cgggcatcct gctgttggtc gtttatgtgg ctgccgctca ggcgaattca cgtttggacg
     2761 actccaacac gaccgcgtgg gcagtgttcg ccctcatggg gctggtcgtg gtcgtggtcg
     2821 tcttcgtgac gggggtcagc gacggttggt ggtccgcggg catcgccgcc gccatcacgg
     2881 cggccgcagc ggtgtgggcg cgcgccgctt cacacccagg gcggccagcg ctgcggcctc
     2941 gttgacagcc aggtgcgcca gcatcggcgc agccaggctg cccgagcgtg cgtgcagcca
     3001 gccgaacaac cagcccccaa ctccggtgag tagcacggtg ccgagcaccg gctgtccggt
     3061 cacgcgggcg tcgacgatgt gccataaccc gaacgccgcg gcctgcagca gccgaccgcg
     3121 cgtcggcccg aatgccgctt cggcgacggt gcccagggtg gcccggtagg cggcctcctc
     3181 gggccagacc gttccgatcg ggatgtcgag caccagccag cgcaccaagt gctcgggcag
     3241 gtcgcgcttt tccatcgccc ggcgcaccgg cggcaacgcg gccgtcgccg cgacaccgag
     3301 cgccgccacc gcgcctgccg cgaggccgta ccgcaggccc gatcgcaacg caggcgggcg
     3361 caagccgagc ggcgattggg tgatcgccac gaggccggtg cccagcgacg cgttagggat
     3421 cggatgccat cgcacgcgcg gcgccacaaa gctccatccc acgagcgccg cagccagccc
     3481 gaccgcgcga aacctgctgg gctgcatcag tattcgggtc cgtcggtgga ttgggtcttt
     3541 tccagcgcgg tgcggatgcg g
//
LOCUS       DASRJA010000464        12977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_491775,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000464 DASRJA010000000
VERSION     DASRJA010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12977
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491775"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VFC01_20450"
     CDS             complement(<1..1043)
                     /locus_tag="VFC01_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN82031.1"
                     /translation="MTLAVSENFEHADVHVAQRLSSLAEETDERVTLAVSLLVRALRG
                     GSVCVDLGSDEWLAAVENSRLAGSPPVLRVHEGLLYFDRYWREEQQVAGDLLTMLPTR
                     VSEAMPDVGRLFPPGFEEQRAAAEIALSQGLTVLTGGPGTGKTTTVARLLALFAEQAE
                     LAGHPRVRIALAAPTGKAAARLQEAVQLEVDRLDASDQQRLQGFQATTLHRLLGWRPE
                     TSSRFRHHRGNRLPHDVIVVDETSMVSLTMMARLLEAVRPETRLVLVGDPDQLASVEA
                     GAVLADVVEGLTNRKDAQIAALQTSHRFGESIGELATAIRVGDADRAVEVLSAGGEHI
                     EWIDADDPREKLR"
     gene            complement(1040..4321)
                     /gene="recB"
                     /locus_tag="VFC01_20455"
     CDS             complement(1040..4321)
                     /gene="recB"
                     /locus_tag="VFC01_20455"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020727458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HZN82032.1"
                     /translation="MKPFDLTGPLPKSRATTVLEASAGTGKTFALAGLVTRYVAEGVA
                     TLDQMLLITFSRAASRELRERVRGQLVDAVAALEGRPVRRSDLVERLLDALPAELDER
                     RHRLRDALAGFDAATIATTHEFCGLVLKSLGVAGDTDAGVQLVESLDDLVTEIVGDIY
                     LCDFGQQPDDPPLSYQDALALARKVVGDPCAKLRPENPEPGTEAAARLAFANKVLDEL
                     DHRKRRLGILHFDDLLSRLAKALEGDESPARERMSQRWPIVMVDEFQDTDPVQWQVIS
                     HAFGGRSAVILIGDPKQAIYAFRGGDIFTYLQAAKTADTQQTLEINWRTDAVLVDRLQ
                     TVLQDAALGHPDIVVRDIRAHHEGHRLAGAPHNDPFRLRVVDRERFGVDDDRVIPIAQ
                     LRAHISKDLAADVAELLDSGATFNGRELTAGDIAVIVERGDDARPCRDALAAMGVPVI
                     YHGDTDVFESDAAEHWLRLLEAFDQPHRSGLVRSAAATPFFGMTADDLAAGGEKLTEQ
                     LTETVRRWSDQARERGIAAVFEAAQVAGMGERLLSLSGGERAMTDLAHVAQLLHDVAH
                     RERFSLPALRDWLRRQRDERNGPPERNRRLDSDAKAVQIMTVWGSKGLQFPVVYLPFA
                     FNRHVWIDDIPLYHDDADVRCLYIGGKDNRGDIDEFSRIEAARNDIRLTYVALTRAES
                     QVVAWWAPADGERNGGLSRLMRGRRAGEITVPDRCDPRTIPDDDALACFREWELRGGP
                     TVEAATITCAVTPQRPAPPSDLGARHFHRDIDTSWRRTSYSALIRSADTPSVGSEPEV
                     IELDDEVGDVPVAKPPTGAEVPSPMAELPTGAKFGTLVHAVLETADPLADDLAAELEA
                     RIDEHSAWWPVDVPAAELAAAMVPMHDTPLGPLADGLTLRQIGLPDRLRELDFELPLA
                     GGDLRAAAPDIQLADVGAMVRAHLPADDPLASYADRLEGAALGGQSLRGYLSGSVDAV
                     LRINGRYVVVDFKTNWLGDSDRPLTAADYDRPRMAEAMLHSDYPLQALLYNVVLHRFL
                     RWRQPGYDPNQHLGGVLYLFLRGMCGPETPVVDGHPAGVFSWQPPASLAVELSNLLDA
                     GRTAA"
     gene            complement(4324..7626)
                     /gene="recC"
                     /locus_tag="VFC01_20460"
     CDS             complement(4324..7626)
                     /gene="recC"
                     /locus_tag="VFC01_20460"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019250068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HZN82033.1"
                     /translation="MALHIHRAERTDLLADGLGALLSTPLPDPFAEELVLVSARGMER
                     WLSQRLSHILGRGNAQDGVCAGISFRSPASLIAEITGTTDDDPWSPDTMTWPLLDAID
                     ASCRERWCRTLATHLGHFDTDPDEKELRQGRRYAVARRLARLFAAYAKQRPQLLVDWL
                     DKNPTDGVGGDLDHDLHWQPHLWRGLVDRVDVDPPHIRHAKTVARLNEGPSDLPSRLS
                     LFGHTRLPATDIELLEALSTHHDLHLWLPHPSDDLWRNLTDAHDRVPRTQDPSRHHAH
                     HPLLDTLGRDLRELQRSLPKGDTDEFIGAAMRPDSLLGWLQSDICANEVRPKGRMHTA
                     ADRSVQVHSCHGAARQIDVLREVLLGLLQDDPTLEPRDILVMCPDIETYAPLIVAAFG
                     LGDIVGDIHPAHHLRVRLADRSLTQTNPLFAVAAQILDIAGSRGTASQVLDLAEAGPV
                     RACFGFTDDDLQTISAWVRQSGIRWGFDAPQRGPFGLDKFLHNTWRFGIDRILTGVAM
                     SDDSQAWLGHALPLDDVGSNKVELAGRFAEFIARLTTAFERLAGPHSLSEWMTALREG
                     VASLTTVDEGQEWQSAQLQREFSKILQQARSRADTELRLADIRALLADHLSGRPTRAN
                     FRTGTLTVCTMVPMRSVPHRVVCLVGLDDGVFPRLDLVDGDDALDRLPMTGERDIRSE
                     DRQLLLDAVCAATETLVVTYNGTDEHSGHHRPAAVPLLELLDALDATTDDPIRKRIVT
                     EHPLQPFDIKNVERGKLIPGEPFTFDPTALRAARAAVGERSAPTAFFSDPLPASPTGD
                     VSLDDLLGFFKHPVRGFFRELDYTLPWEVEAVEDAMPVEITALQEWEVGDRLLTDLMR
                     GTDLEFATGAEWRRGTLPPGRLGWRKALAISDCAADLAMAALRPRGAHRATGHDIDVD
                     LGSRRVTGTVMPVYGDRIISVTYSRLAAKQILQSWISLVALKAQMPDREWAAFCVGRD
                     AQAVIKVRMFRPPAEPTTVLRDLVAIFDAGRREPLPLPLKTSYAWAQKRHEGREPRWF
                     ANSKWESPRNMAISEDADKAHVEVWGSRAPLTKLLAAQPHPGEEFDGEDTRLGALAAR
                     LWLPVFDAEEQV"
     gene            complement(7966..8583)
                     /locus_tag="VFC01_20465"
     CDS             complement(7966..8583)
                     /locus_tag="VFC01_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZN82034.1"
                     /translation="MSEQTSTPKLTGQFSKALVYAELKHHNQVRKGGDIPYVGHLLTV
                     AGLVINDGGSEAQAIAALLHDAVEDAGGPPTLEEIRKNFGDDVARIVDECSDTDVEPK
                     PPWRERKQNYINHLAAVGDDTLLVSVADKLDNARSMLRDYHEHGPKLWERFTVKDPRD
                     HLWYFGELLAAYRRRGLTSWMVDELGRVVDELKRLVEGGDRIVLV"
     gene            8706..8918
                     /locus_tag="VFC01_20470"
     CDS             8706..8918
                     /locus_tag="VFC01_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009935474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HZN82035.1"
                     /translation="MARTNIDIDDSLVSEVMRKFGVSTKKEAVDLALRRLVGAPLTKE
                     FLLGLEGVGWDGDLDALRREKPDEFA"
     gene            8915..9316
                     /locus_tag="VFC01_20475"
     CDS             8915..9316
                     /locus_tag="VFC01_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HZN82036.1"
                     /translation="MILIDTSAWIEYFRATGSAAAVEVRRLLSEEADQVATCEPVAME
                     ILSGVNDDTHAKLERLVNGLPSLNIDSSIDFRTAAQIYRGARRAGRTIRSINDCLIAA
                     VAIRHDVRIIHQDNDFDVIAAMTGLVATSLK"
     gene            complement(9320..9982)
                     /locus_tag="VFC01_20480"
     CDS             complement(9320..9982)
                     /locus_tag="VFC01_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82037.1"
                     /translation="MTRRCALGWLRASAVVTLVGAVALPGQASAAPGATVTITVKAVA
                     ANGDPVNGYQVTNRQSSPNLSGCLAPSPAAVGSNIYACEPAQAAAQVCWPAPASVLCL
                     ADPWSKGLRRFASPGALPAVEPMATPMPFALQLDNGTRCILPNGIDWGARADDMVPAY
                     GCNPGKTSMAVLIPPGADPVSAIDRSAPVWVVRVGELGPRDTPFESPRRHAVTTAWFA
                     GN"
     gene            10049..11215
                     /locus_tag="VFC01_20485"
     CDS             10049..11215
                     /locus_tag="VFC01_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN82038.1"
                     /translation="MKAVTCTNAKLDVVDQPTPTPAKGQLLLDVLRCGICGSDLHARN
                     HCDEVAEVMAETGYPDFMRSPQSVVFGHEFCGEVLDHGPGTRKAPKAGTPVVALPLLR
                     RGKDVHALGLSTKAPGAYAEQLVVEQSLTLPIPNGLPADKAALTEPMAVGWHAVQRAD
                     IGKKDVAIVIGCGPIGLAVILMLKAHGVGTVIASDYSPGRRALATQCGADVVVDPAQD
                     SPYGKANGHGHLETPLDAFDLAIGTLEKLQRARLPWWHVWRAADKLGAASPKRPVVFE
                     CVGVPGIIEDIITGAPLFSRVVVVGVCMGADKLRPSMAINKEIDLRFVLGYTPLEFRD
                     TLHMLAEGKVDVTPLVTGTVGLPGVAAAFDALGDPEAHAKILIDPKSDAASPTP"
     gene            complement(11212..12459)
                     /locus_tag="VFC01_20490"
     CDS             complement(11212..12459)
                     /locus_tag="VFC01_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82039.1"
                     /translation="MATSALRRIVDPVIAAPPAGVRVRTRLKVTAAEAATLTTIGLFL
                     GSVYRSELAGRIRCGVLGPDGQRAWRAERKRAITAVSSSRWAGAITRTVEDQYQLGMR
                     SLTAQVTNLRAAISVLEPRCALRPGELAPVDHDDAGTKRPSRRQRGYRNKAERFVKTR
                     RLAALRERLVTAERTLAAGCPSITVGGKRFWRNRNNLDAAELSEQQWRQQWDSCRMFF
                     TADGEAGRTGGNETIRVDGDGHLRIKTPSDLVGELGSHVVIAAPVRFSFRGEEWAARV
                     AARQAVRYDLSYDSIRGRWYIDASWTITPEPMCELAELRTTRVLGVDLNVDDLACCIL
                     DASGNPIGRPTNIELPTVEMSASRRDGRVRAAITALLDHAQVAGCGAVVVENLGFADD
                     NQERLACRQVGAWRRQLVKLLSV"
     gene            complement(12556..>12977)
                     /locus_tag="VFC01_20495"
     CDS             complement(12556..>12977)
                     /locus_tag="VFC01_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HZN82040.1"
                     /translation="IAAVVFMAFAGIVGWFIVSKSTRSIPNFPGVTIPSISVPSMPST
                     PEGPTTAPPGGTLSVSGMGATKTLECNDNRVTISGMGNIVTITGHCTTVTVSGMQNKV
                     TIDTADQISASGVNNNVTYHSGSPDINNAGNNVVEQG"
BASE COUNT         2152 a         4489 c         4153 g         2183 t
ORIGIN      
        1 ttgcgcaact tctcgcgcgg atcgtccgcg tcgatccact cgatgtgctc gccgccggca
       61 ctcagcacct cgacggcccg gtccgcgtcg ccgacccgga tcgcggtggc caactcgccg
      121 atcgactcgc cgaagcggtg cgacgtctgc agcgccgcga tttgtgcgtc tttgcggttc
      181 gtcaacccct cgaccacgtc cgccaacact gcgcccgcct ccaccgacgc caactggtcg
      241 gggtcgccga ccagcaccag ccgcgtctcc gggcgcaccg cctccaacag ccgcgccatc
      301 atcgtcagcg acaccatcga cgtctcgtcg accacgatga cgtcatgggg caaccggttc
      361 ccgcgatggt gccggaaccg cgacgacgtc tccggccgcc accccagcag ccgatgcagc
      421 gtggtcgcct gaaaaccctg aagccgctgt tggtccgaag catccagccg gtcgacctcc
      481 agctgcaccg cctcctgcaa ccgcgccgcc gccttgcccg tcggcgccgc caaggcgatt
      541 cgcactcgcg gatgtccggc caactccgcc tgctcggcga acaacgccaa caatcgcgcg
      601 accgtcgtcg tcttacccgt ccccggccca cctgtcagca ccgtcaaccc ttgcgacagc
      661 gcgatttccg cggccgcccg ctgttcctcg aaccccggcg ggaacaagcg gccgacgtcc
      721 ggcatcgcct ccgacaccct cgtcggcagc atggtcagca gatcaccggc gacctgttgt
      781 tcctcgcgcc aataccgatc gaagtacagc agcccctcgt ggacccgcag caccggcggt
      841 gaacctgcga gccgactgtt ttcaacggcg gccaaccact cgtccgagcc caggtcgaca
      901 cacaccgaac ccccgcgcag cgcccgcacc aacagcgaca ccgccaacgt cacgcgttcg
      961 tcggtctcct ccgccagaga cgacagccgt tgcgccacat gtacatcggc atgctcgaag
     1021 ttctcactga ccgccagtgt catgccgccg tcctccccgc gtcgagcaga ttggacaact
     1081 cgaccgccaa cgacgccggt ggctgccaac tgaacacccc ggccgggtgc ccgtcgacca
     1141 ccggagtctc tggtccgcac atcccgcgca ggaacagata cagcaccccg cccaagtgtt
     1201 ggtttgggtc gtagccaggc tgccgccacc gcagaaagcg atgcagtaca acgttgtaca
     1261 gcagcgcctg cagcggataa tccgagtgca gcatcgcctc ggccatccgc ggccggtcgt
     1321 agtccgcggc ggtcagcggc cgatcggagt cgcccagcca gttcgtcttg aaatccacca
     1381 ccacgtatcg gccgttgatc ctcagcaccg cgtcgaccga cccgctgagg tatccccgca
     1441 acgactgccc gcccagcgca gcgccttcca accggtccgc gtacgacgcc agcggatcgt
     1501 cggccggcag atgcgcccgt accatcgcac cgacatcggc cagttggata tccggcgctg
     1561 ccgcccgtag atcgccaccg gccagcggca actcgaagtc caactcgcgc aatcgatccg
     1621 gcaatccgat ctgacgcagt gtcaacccat ccgccagcgg gccaagggga gtgtcatgca
     1681 tgggcaccat cgccgccgcc aactccgctg cgggcacatc gaccggccac cacgccgagt
     1741 gctcgtcgat ccgcgcctcc agctcggccg caaggtcgtc ggcgagcgga tcggccgtct
     1801 ccagcactgc gtgcaccaac gtgccgaact tcgctccggt gggtagctcg gccatcggcg
     1861 acggcacctc cgcgcccgtc ggcggcttgg ccaccggcac gtcgccgacc tcgtcgtcca
     1921 gctcgatgac ctctggctcg ctgccgacgc taggtgtgtc ggcgctgcgg atcaatgccg
     1981 agtacgacgt gcgccgccac gaggtgtcga tgtcgcggtg gaaatggcgc gccccgagat
     2041 ccgatggcgg cgcgggccgt tgcggggtca ccgcacaggt gattgtcgcg gcctcgaccg
     2101 tcggtccgcc gcgcagctcc cactcccgga agcacgccag cgcgtcgtcg tcggggatcg
     2161 tgcgcgggtc gcagcggtcc ggcacggtta tctcgccggc gcgacgcccg cgcatcagcc
     2221 gcgacaggcc gccgttgcgt tcgccgtccg ccggtgccca ccacgcgacc acctgcgact
     2281 ccgcccgcgt cagcgcgaca tacgtcaggc gaatatcgtt gcgggcggcc tcgatccggc
     2341 tgaactcgtc gatatcaccg cgattgtcct tgccgccgat atacaggcac cggacgtcgg
     2401 cgtcgtcgtg gtacagcggg atgtcgtcga tccagacgtg ccggttgaac gcaaagggca
     2461 ggtacacgac cgggaattgc agccccttgc tgccccacac cgtcatgatc tggaccgcct
     2521 tggcgtcgct gtccagtcgt cggttgcgtt ccggtgggcc gttgcgttcg tcgcgctggc
     2581 ggcgcagcca atcccgcagc gccggcaggc tgaaccgctc gcggtgggcc acgtcgtgca
     2641 gcagttgtgc gacgtgcgcg aggtcggtca tcgcccgctc gccgccgctg aggctcagca
     2701 ggcgctcgcc catgcccgcg acctgcgcgg cctcgaacac cgcggcgatt ccgcgttcgc
     2761 gcgcctgatc cgaccaccgc cggacggtct cggtcagctg ctcggtcagt ttctctccgc
     2821 cggcggcgag gtcgtcggcc gtcatcccga agaacggcgt cgccgcggcc gatcgcacca
     2881 gtccgctgcg gtgcggttgg tcgaacgctt cgagcagccg caaccagtgt tcggctgcgt
     2941 cggactcgaa cacgtcggtg tcgccgtgat agatcaccgg aacgcccatg gccgccaagg
     3001 catctcggca tggcctggca tcgtcacctc tctcgacgat gaccgcgatg tcgcccgccg
     3061 tcagttcccg accgttgaac gtcgctccac tgtcgagaag ctctgctacg tcggcggcta
     3121 gatctttcga gatatgggcg cgcagttggg cgatcgggat gacccggtcg tcgtccaccc
     3181 cgaatcgttc gcggtcgacc acccgcagcc gaaacgggtc gttgtggggc gcgccggcca
     3241 ggcggtggcc ctcgtggtgg gccctgatgt cacggacaac gatgtcgggg tggcccagtg
     3301 cggcatcctg caagaccgtc tgcagccggt cgaccagaac cgcgtcggtg cgccagttga
     3361 tctccagagt ctgttgcgta tcagcggttt tcgcggcctg cagatacgtg aagatgtcgc
     3421 cgccacggaa cgcgtagatc gcctgcttcg ggtcgccgat cagaatgacc gccgaccgtc
     3481 cgccgaatgc gtgcgagatg acctgccact gcaccgggtc ggtgtcctgg aactcgtcga
     3541 ccatgacgat cggccaccgc tgcgacatgc gttcccgcgc gggggactcg tcaccctcaa
     3601 gggctttcgc caaccggctc aacagatcgt cgaagtgcag aatgccgagc cgacgtttgc
     3661 ggtggtcgag ttcgtcgagc accttgttgg cgaaggccag ccgcgccgcc gcctcagtcc
     3721 cgggctctgg gttctccggc cgcagcttcg cgcagggatc gccgacgact ttgcgtgcca
     3781 gcgccaaagc gtcctggtag ctcaacggcg gatcgtctgg ctgttggccg aagtcgcaga
     3841 gatagatgtc gccgacgatc tcggtcacca ggtcgtcgag gctttcgacc agttgtacac
     3901 cggcatcggt atccccggcc acaccaagcg atttcaagac cagcccgcag aactcgtgtg
     3961 tggtggcgat ggtcgccgcg tcgaatccgg ccaacgcatc tcgcaaccga tgccgccgtt
     4021 cgtcgagctc tgcgggtaag gcgtccaata gacgctcgac gagatcgctg cgtcgcaccg
     4081 gcctgccctc cagggccgcg acagcgtcga cgagctgacc tcggactcgc tcgcgcagtt
     4141 cgcggctggc ggcgcggctg aacgtgatga gcagcatctg gtcgagcgtc gcgacgccct
     4201 ccgcgacgta gcgcgtcacc aggcccgcga gcgcgaacgt ctttcccgtc ccggcgctcg
     4261 cctccagcac cgtcgtggcg cgcgatttcg gcagcgggcc ggttaagtca aagggtttca
     4321 ccgctacacc tgttcctcgg cgtcgaacac cggaagccac agccgagcgg cgagcgcgcc
     4381 caaccgggtg tcttcgccat cgaactcttc accggggtga ggctgcgcgg caagcaactt
     4441 cgtcaacggg gctctcgaac cccacacctc gacgtgcgcc ttgtcggcgt cctcgctgat
     4501 ggccatgttg cgcggacttt cccacttgct gttcgcaaac cacctgggtt cgcggccctc
     4561 gtgacgcttc tgcgcccacg cgtacgacgt cttcagcggc aacggcagcg gttcgcgtcg
     4621 cccggcgtcg aagatggcca ccaggtcacg cagcaccgtc gtcggctcgg caggcgggcg
     4681 gaacatccgc accttgatca cggcttgcgc gtctcggccg acgcagaacg ccgcccactc
     4741 gcggtcgggc atctgcgctt ttagggccac aagcgaaatc catgactgga gaatttgttt
     4801 ggccgccagt cgcgagtacg tcaccgagat gattcgatcg ccgtagacgg gcatcacggt
     4861 tccggtgacg cgacggctgc cgaggtcgac gtcgatgtcg tggcccgtcg cgcggtgcgc
     4921 gccgcggggg cgcaatgccg ccatcgccag gtcggccgcg caatcgctga tggccagcgc
     4981 cttgcgccag ccgagcctgc cgggcggcaa cgtgccgcgg cgccattccg cgccggtggc
     5041 gaattcaagg tcggtgccac gcatcaggtc tgtcagcagc cggtcgccga cctcccattc
     5101 ctgcagcgcg gtgatctcga cgggcatcgc atcctcgacg gcctccacct cccacggcag
     5161 cgtgtagtcg agctcgcgga agaatccccg caccggatgc ttgaagaacc ccagcaggtc
     5221 atcgagcgag acgtcaccgg tgggcgatgc gggcagcggg tcggagaaga acgctgtcgg
     5281 tgcggaccgc tcgcctacag ccgcgcgcgc ggcgcgcagg gctgttggat cgaaggtgaa
     5341 cggctcaccg gggatgagct ttccgcgctc gacgttcttg atgtcgaacg gctgcaacgg
     5401 atgttcggtg actatgcgtt tacggatcgg atcgtcggtg gtcgcatcca gcgcgtcgag
     5461 cagttcaagc agcggcacgg ccgcgggtcg gtgatggccg gagtgttcgt cggtgccgtt
     5521 gtaggtgacg acgagtgtct cggtggccgc gcagaccgcg tcgagcagca gctggcggtc
     5581 ctcggagcgg atgtcgcgct ccccagtcat gggcaggcgg tccagcgcgt cgtcgccgtc
     5641 gacgaggtcc agccgcggga acacgccgtc gtcgaggccg accaggcaca cgacccggtg
     5701 aggcaccgaa cgcatcggca ccatggtgca tacggtcagc gtcccggtgc ggaagttcgc
     5761 ccgggtgggc cgaccggaga gatggtcggc gagcaatgcg cggatatcgg cgagccgtag
     5821 ctcggtgtcc gcacgagatc gagcctgctg caggatcttt gagaattcgc gttgcagctg
     5881 agccgactgc cattcctgtc cctcgtcgac tgtggtgagc gacgcgacac cctcgcgcag
     5941 cgccgtcatc cactcggaca atgaatgcgg gcctgcgaga cgttcgaagg cggtcgtgag
     6001 ccttgcgatg aactcggcga accggccggc caattcgacc ttgttgctgc cgacgtcgtc
     6061 gaggggcagg gcgtggccga gccatgcctg tgaatcgtcg gacatcgcga cgccggtgag
     6121 aatgcggtcg atcccgaatc gccacgtgtt gtgcaggaac ttgtccagtc cgaacggccc
     6181 tcgctgtggc gcgtcgaagc cccatcggat gccggattgg cgcacccacg ccgagatcgt
     6241 ctggaggtcg tcgtcggtga acccgaagca cgcgcgcacg ggccccgcct ccgccaggtc
     6301 gagcacctgg ctggcggtgc cccggctgcc agcgatgtcg agaatctgtg cggcgacggc
     6361 gaatagggga tttgtttggg tcaacgaccg gtcggcgagc cggacgcgca agtgatgcgc
     6421 cgggtggatg tcgccgacga tgtcgccgag gccgaacgcg gcgacgatca gcggcgcgta
     6481 ggtctcgatg tcggggcaca tgacgaggat gtcgcggggt tcgagcgtgg ggtcgtcctg
     6541 cagcaggccg agcaacacct cacggagcac atcgatctga cgagcggcgc cgtggcagct
     6601 gtgcacctgc accgagcggt ccgctgcggt gtgcatgcgt cctttgggcc ggacctcgtt
     6661 ggcgcagatg tcggattgca gccagcccaa cagggaatcg ggacgcattg cagcaccgat
     6721 gaattcgtcg gtgtcgcctt ttgggaggct gcgttgcagc tcgcgcaggt cgcggccgag
     6781 ggtgtcgagc agcggatggt gggcgtggtg gcggctgggg tcctgggtgc gcggcacgcg
     6841 gtcgtgagcg tcggtgaggt tccgccacag gtcgtcgctg gggtgtggca gccacagatg
     6901 taggtcgtgg tgcgtcgaca gcgcttcgag gagctcgatg tcggtggcgg gaaggcgggt
     6961 gtgcccgaac agcgacaggc gcgacggcag gtcgctgggg ccttcgttta gccgggcgac
     7021 ggttttggcg tggcggatat gcggcggatc gacgtcgacg cggtcgacca ggccgcgcca
     7081 gaggtgcggc tgccagtgca ggtcgtggtc gaggtcgccg ccgacgccgt cggtggggtt
     7141 cttgtcgagc cagtcgacga gcagctgcgg ccgctgtttg gcgtatgccg cgaacagtcg
     7201 ggccaggcgt ctggcgacgg catagcgccg gccttggcgc agctccttct cgtcggggtc
     7261 ggtgtcgaaa tgcccgagat gtgttgcgag cgtgcggcac caccgttcgc gacagctggc
     7321 gtcgatggcg tcgagcagcg gccaggtcat ggtgtcgggt gaccatgggt cgtcgtccgt
     7381 ggtgccggtg atctcggcga tcagcgaggc gggggaacga aacgagatgc cggcgcagac
     7441 gccgtcctgg gcgttcccgc ggccgaggat gtgcgagagc cgctgactca gccaacgctc
     7501 catgccgcgc gccgagacga gcaccagttc ctcggcgaac gggtcgggca ggggagtgga
     7561 cagcagggcg ccgagaccgt cggcgaggag atcggtgcgc tcggcacgat gaatgtgaag
     7621 cgccatcgag ggccaccata ggtcggactg gcgaccaccc gcggcatcac cgcggacatt
     7681 gcggaacttc tctgtcagtc cgacgccccg gatactgggg agacagcggc caggacggat
     7741 gacggagggt gaccatgacg acggctgacg atcgcagcca gaaccgtcgc ggatggctac
     7801 ccccgcggcg cggcggctat atccctcgtg ccgatacgcc taatggcaga ggtgtcaagc
     7861 caccccaggg cggtggtggc gtcgggaatc ttccccgccg agccgggaca gaccgaagcc
     7921 agacaagcgt ctcgtggcaa tggcggccgg tgaagccatc gaggctcaga ctaggacgat
     7981 cctgtcgccg ccttcgacga gacgcttcaa ctcatcgacg acgcggccga gttcgtcgac
     8041 catccagctg gtgaggccgc ggcggcggta ggcggcaagc agttcgccga agtaccaaag
     8101 gtggtcgcgc ggatccttga cggtgaagcg ctcccagagc ttcgggccgt gttcgtgata
     8161 gtcgcgcagc atcgaccgcg cattgtcgag cttgtcggcg accgaaacga gcagagtgtc
     8221 gtcgccaacc gcggcgaggt gattgatgta gttctgcttg cgctcacgcc acggcggttt
     8281 gggttccaca tcggtgtcgc tgcactcgtc gacgatgcgc gcgacgtcgt cgccgaagtt
     8341 cttcctgatc tcctcgagcg tgggcggccc gccagcgtct tcgaccgcgt catgcaacag
     8401 cgccgcgatg gcctgcgctt ctgagccgcc gtcgttgatc accagcccgg cgaccgtcag
     8461 cagatggccg acgtacggga tgtcgccgcc cttgcgcact tggttgtggt gcttcagctc
     8521 ggcgtacacc agcgccttcg aaaactggcc cgtcagcttc ggtgtcgagg tttgctcact
     8581 cacttctcgg tgtcctctct attcagttcg accatagcga gacaggacga catcgatatt
     8641 tgcccgcctc tgggcgacaa ggtcgccgcc aacgacaaca gagagctgat gtgtatgctt
     8701 ggtgtatggc acgcacaaac attgatatcg acgactcttt ggtctcggag gtcatgcgca
     8761 agttcggcgt gtccacgaag aaggaggccg tcgatctcgc ccttcggcgg ctcgtcggtg
     8821 cgccgctgac gaaggaattc ctcttggggc tcgaaggcgt cgggtgggat ggtgacctcg
     8881 acgcgctgcg ccgcgagaag ccagacgaat tcgcatgatt ctgatcgaca catctgcgtg
     8941 gatcgagtat tttcgcgcaa cggggtcagc cgccgcagtc gaagtgcgac gattgctttc
     9001 ggaggaggcg gaccaggtgg cgacgtgcga gcccgtcgcc atggaaatcc tgtcgggcgt
     9061 caacgacgac acccacgcga aactcgagcg actcgtcaac ggcttgccgt cgttgaacat
     9121 cgacagctcg atcgatttcc gtacagccgc acagatctat cgtggggccc gtcgcgccgg
     9181 tcgaaccatt cgcagcatca acgactgttt gatcgcagcg gtagcgatcc gccatgacgt
     9241 ccgaataatt catcaagaca acgacttcga tgtcatcgca gcgatgacag ggcttgtggc
     9301 tacctcattg aagtagcact cagttccccg cgaaccatgc cgtcgtgacc gcatgtcgtc
     9361 gcggcgactc gaacggcgta tcgcgcggcc ccaattcgcc gactcgaacc acccacacag
     9421 gtgccgaccg gtcgatcgcc gacaccggat ccgcgcccgg cggaatcaac accgccatcg
     9481 acgtcttgcc cggattgcac ccgtatgccg gcaccatgtc gtccgcccgc gcgccccagt
     9541 cgatcccgtt cggcaggatg catcgcgttc cattgtcgag ctgcagcgcg aacggcatcg
     9601 gcgtcgccat cggctccacg gccggcaacg ctcccggcga cgcgaatcgg cgcagcccct
     9661 tgctccatgg atccgccagg cacaacaccg acgccggtgc cggccaacac acttgggccg
     9721 ccgcctgagc cggctcgcag gcatagatgt tcgatcccac cgccgccggc gacggagcca
     9781 ggcaccccga cagattcggc gaactctgcc gattcgtcac ctgatatcca ttgaccggat
     9841 cgccattcgc agcgacggcc ttgaccgtga tggtcaccgt cgctcccggt gcggcactgg
     9901 cctgaccggg cagcgcgaca gccccgacga gcgtcaccac cgccgacgct cgcaaccacc
     9961 cgagcgcaca ccgccgtgtc acgagagcct ccgattccag gatggcttat ttggcttgtc
    10021 tataaacccg aatcgctagg ctcggccaat gaaggccgtc acctgtacca acgccaagct
    10081 cgacgttgtc gaccagccca ccccgacacc ggcgaagggc cagctgcttc tcgacgtcct
    10141 ccgctgcggc atctgcggct cggacctgca cgcccgcaac cactgcgacg aggtcgccga
    10201 ggtgatggcc gaaaccggct accccgactt catgcggtcg ccacaatcgg tcgtcttcgg
    10261 ccacgagttc tgcggcgaag tcctcgacca cggccccggc acccgtaagg caccgaaagc
    10321 cggcacgccc gtcgtcgccc tgcctctgct ccgccgcggt aaggacgtgc acgcactcgg
    10381 cctctccacg aaagcccccg gtgcctacgc cgaacagctc gtcgtcgagc agtccctcac
    10441 gctgcccatc cccaatggcc tgcccgccga caaagccgcc ctgaccgagc ccatggccgt
    10501 cggctggcac gcggttcagc gcgccgacat cggcaagaaa gacgtcgcca tcgtcattgg
    10561 ctgcggaccg atcgggcttg ccgtcatcct catgctcaag gcccacggcg tgggcaccgt
    10621 catcgccagc gactactcgc ccggccgccg cgcactcgcc actcaatgcg gcgccgacgt
    10681 cgtcgtcgac ccggcccagg actcgcccta cggtaaggcg aacggccacg gccacctcga
    10741 aaccccgctc gacgccttcg atctcgcgat cggcacgctc gagaagctcc agcgcgcacg
    10801 cctgccctgg tggcacgtct ggcgcgcggc cgacaaactc ggcgccgcct cacccaagcg
    10861 gcccgtcgtc ttcgaatgcg tcggcgtgcc cggcatcatc gaggacatca tcaccggcgc
    10921 tccactgttc tcccgcgtgg tcgtggtcgg tgtctgcatg ggcgccgaca agctccggcc
    10981 gtcgatggcg atcaacaaag agatcgacct gcgcttcgtg ctcgggtaca cgccgctgga
    11041 gttccgcgac acgctgcaca tgctcgccga gggcaaggtc gacgtcaccc cgctcgtcac
    11101 gggcaccgtc ggattgcccg gcgtggcagc cgcattcgat gcgctcggcg atcctgaggc
    11161 gcacgccaag atcctgatcg atcccaagag cgacgccgcg agcccgacgc cctagactga
    11221 caacagcttc acgagttgcc gtcgccaagc tccgacttga cggcacgcca accgttcctg
    11281 attgtcgtcg gcgaaaccca gattctccac cacgaccgcc ccacagccgg cgacctgagc
    11341 gtgatcgagc agggcggtaa tcgccgcgcg tacccgcccg tcacgccgcg acgctgacat
    11401 ctccacggtg ggtagctcga tatttgttgg tctcccaatc gggttacccg aggcatccaa
    11461 gatgcagcag gccagatcgt cgacgttgag atcgaccccg agcacgcgcg ttgtcctcaa
    11521 ctcagccaac tcgcacatcg gctcgggagt aatggtccag gatgcgtcta tgtaccaacg
    11581 gccccgtatc gagtcatagc tcaggtcgta gcgcaccgcc tgccgcgcgg ccactcgcgc
    11641 agcccattct tcgccgcgaa agctaaaccg aaccggggcg gcgatcacca catgtgagcc
    11701 cagctctccg acaagatcgg acggagtctt gatccgcagg tgtccgtctc catcgacgcg
    11761 gatagtttca ttaccgcccg tcctacctgc ctccccatcg gcggtgaaga acatccggca
    11821 tgaatcccac tgctgccgcc actgctgctc gctcaactcc gcggcgtcga ggttgttgcg
    11881 gttgcgccag aaccgttttc cacccaccgt gatcgacgga caccctgctg ccaacgtccg
    11941 ctcggcagtc accaggcgct cgcgtagtgc ggccagtctc cgagtcttta cgaaccgctc
    12001 tgccttgttg cgatagccac gttgtcgccg agatggacgc ttcgtgccgg cgtcgtcgtg
    12061 gtcaactggg gccagttcac cggggcgcag cgcacaccgc ggctctagaa cgctgatcgc
    12121 agcgcgcaga ttcgtcacct gcgcagtcaa cgaccgcatc ccgagctggt attggtcctc
    12181 gacggtgcgg gtaatcgcac cggcccaccg cgacgacgac accgcggtga tcgcccgctt
    12241 acgctcggca cgccaggccc gctggccgtc tggacccagc acgccgcagc ggatcctccc
    12301 tgcgagttcg ctgcgataca ccgatcccaa gaacaaaccg atagtcgtca acgtcgcggc
    12361 ctctgccgcg gtcaccttca gccgggtgcg cactcggacc cccgctggag gcgcagctat
    12421 cacaggatca acgatccgcc gcaacgcact cgtggccacc gcgacatcat ggcagcgacc
    12481 accgacaaac cacctacctg cggcgctcaa cttactcgcg gcagcactca cttccgctca
    12541 gatgcgtgac cgagctcagc cctgttcgac cacgttattc ccggcgttat tgatgtccgg
    12601 tgagccggag tggtaggtga cgttgttgtt gacccctgag gcgctgatct gatcggccgt
    12661 gtcgatcgtg accttgttct gcatgcccga caccgtgacc gtcgtgcagt gacctgtgat
    12721 cgtgacgatg ttgcccatgc cgctgatcgt cacgcggttg tcgttgcatt cgagcgtttt
    12781 ggtcgcgccc atgccggaga cgctgagtgt gccaccgggc ggtgcggtgg tcgggccttc
    12841 gggcgtgctc ggcattgacg gcacgctgat actcgggatg gtgacgccgg ggaagttggg
    12901 gatcgagcgc gtcgacttgc tcacgatgaa ccacccgaca atcccggcga aggccatgaa
    12961 aacgacggca gcgatgc
//
LOCUS       DASRJA010000465         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3511982,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000465 DASRJA010000000
VERSION     DASRJA010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3511982"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFC01_20500"
     CDS             <1..271
                     /locus_tag="VFC01_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HZN82041.1"
                     /translation="PLLSCTIITTDAAGPLADIHDRMPLTISRDDWDRWLDPDAPVDE
                     GLLRGHGDLDRIAIREVSRLVNSIRNNGPELIEPAAPEAEQATLL"
     gene            311..904
                     /locus_tag="VFC01_20505"
     CDS             311..904
                     /locus_tag="VFC01_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82042.1"
                     /translation="MPRPGASDARGRLLEVARRRFAVDGALVPTLDEVRREAEVSVGA
                     LYHHFPDKPALAAAVYAQLIGEYQAGFVAMLREHDTAEGGIRGGVGYHLRWVAEHRNE
                     AGLLLGDRLDSAALRDSNRDFFAVVGDWWRPHHAYGVLRPMRLGVTAALWLGPAQEYS
                     RYWIAGGDAKIPRGTVEIFATAAWAALRANDIEEQVQ"
     gene            901..1725
                     /locus_tag="VFC01_20510"
     CDS             901..1725
                     /locus_tag="VFC01_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN82043.1"
                     /translation="MNSMYRAMVATGDDPDAPERVHVDHRGDRAVITLDESDRLNVLS
                     APLTRQLRRALQALDADADIRSVVITGADPGFSAGGDLKMMKVAIESLGAQEGVADVW
                     RWIRREFGGIARLIAGSDTIFLAALNGAAAGVGLAWALTCDLVVASDRAVIVPAFGRL
                     GLLPEVGTSWALTRRLGYQGALAYYLRGEHIDANTALRLGLVQEVVEHDQLLAAADEW
                     CSRIANLPPHAVAMAKPLLRATADADWNATLTLEEFAEPACFTTAAFADSVRSLLK"
     gene            1815..2000
                     /locus_tag="VFC01_20515"
     CDS             1815..2000
                     /locus_tag="VFC01_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82044.1"
                     /translation="MLSTLRRVLSFEMTIAEWIGTALMLAVPYLAVGVVWSLAHNWHL
                     GSIVWWPVQLVTTVCLA"
     gene            1997..2862
                     /locus_tag="VFC01_20520"
                     /pseudo
     CDS             1997..2862
                     /locus_tag="VFC01_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208836.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
BASE COUNT          484 a          986 c          996 g          546 t
ORIGIN      
        1 gccgctgctc agctgcacga tcatcaccac cgatgccgcg ggaccgctgg cggacatcca
       61 tgaccggatg ccgttgacca tcagccgcga cgactgggac cgctggctcg atcccgacgc
      121 ccccgtcgat gagggcctgc tgcgcggcca cggcgacctc gatcgcatcg cgatccgcga
      181 ggtgtcgcgc cttgtcaaca gtatccgcaa caacgggcct gagctgatcg agccggccgc
      241 gccggaggct gagcaagcga ctctgctgta gtcctaccga ggatttctcg gtacgactag
      301 agtattcctc gtgccacgcc caggtgcttc ggatgcgagg ggccggttgc tggaagtggc
      361 acggcgccgg ttcgccgtcg acggcgccct cgtgcccaca ctggacgagg tccgtcgcga
      421 ggccgaggtg agcgtcggcg cgctgtacca ccacttcccg gacaagcccg cgctggccgc
      481 cgccgtctac gcgcagttga tcggcgagta ccaagcggga ttcgtcgcca tgctgcgcga
      541 gcacgacacc gcggaaggcg gcatccgagg cggtgtcggg tatcacctgc ggtgggtggc
      601 cgagcaccgc aacgaggccg ggttactgct cggcgaccgg ctggacagtg cggcgttgcg
      661 cgactccaac cgggacttct tcgccgtcgt cggggactgg tggcgtccgc accacgccta
      721 cggtgtgctg cggccgatgc gcctcggagt cacggccgcg ctctggctcg ggccggcaca
      781 ggaatacagc cgctattgga tcgcgggtgg cgacgccaag ataccgcgcg gcactgtcga
      841 gatcttcgcc accgccgcgt gggcggcgct gcgcgccaac gatattgagg agcaagtgca
      901 atgaattcga tgtaccgcgc aatggtggcg accggcgacg atcctgacgc gcccgagcgg
      961 gtgcacgtcg atcaccgcgg tgatcgggcc gtaatcacgc tcgatgaatc cgaccgactc
     1021 aacgtgcttt ccgccccgct gacgcgtcaa ctccggcggg cgctgcaagc cctcgatgcc
     1081 gacgctgaca tccgctccgt cgtcatcacc ggcgccgatc ccggattcag cgccggcggt
     1141 gacctgaaga tgatgaaggt cgcgatcgaa tcgctcggcg cacaggaggg tgtggccgac
     1201 gtgtggcggt ggatccgccg cgagttcggc ggcatcgccc ggctcattgc ggggtcggac
     1261 acgatcttcc tggccgcgct caacggcgcg gcggccggcg tgggtctggc gtgggcgctg
     1321 acatgcgacc tcgttgtcgc cagtgaccgc gcggtgatcg tgcccgcatt cggccgcctc
     1381 ggactgctcc ccgaggtcgg caccagttgg gcgctcaccc gccgactggg ctaccaggga
     1441 gcgctcgcgt actacctgcg cggcgagcac atcgacgcca acacagcgct tcggctcggg
     1501 ctcgttcagg aggtcgtcga gcacgaccaa ctgctggccg ccgctgatga atggtgctcg
     1561 cgcatcgcga atttgccgcc gcacgcggtc gctatggcca agccgttgct ccgcgccacc
     1621 gccgacgccg actggaacgc cacgctcacg ttggaggaat tcgccgaacc ggcgtgcttc
     1681 accaccgcgg cgttcgccga cagcgtgcgg tctttgctca agtaggtacc agcgttcaca
     1741 tcgagcgcat ttgctcactc agacaatcac aaattgggcc atgccacctc gaatcgccat
     1801 taacataatg ggtcatgctc agcacactga gaagagtctt gagctttgag atgacgatcg
     1861 ccgagtggat cggcacggct ctcatgctcg cagtgccgta tctggccgtc ggggtggtct
     1921 ggtcacttgc gcacaactgg cacctcggct cgatcgtgtg gtggccggta caactggtca
     1981 cgacggtgtg cctggcatga cccccgccga ctgccgtatc gacatccgcc gacgcaccgc
     2041 gcgctgggat ctggccgccg aggtctctgc ggccgaggcc atggatttct gggcgttcgc
     2101 cgccggggcc gccaacgtcg tcatgcaact gtcgcggccg gaagtgggct acggcgtcgt
     2161 cgagagcaag gtggattccg gaaatctgct gaagcatccg tggaaacggg ccaggacgac
     2221 gtttcagtat ctggcggtag cgattctggg ccgaccggac gaccgtgccg cattccgcga
     2281 ggccgtcaac ggttcacatc gccacgtcaa gtccagcccg gacagtccgg ttcgctacaa
     2341 cgccttcgat cacgaactcc agatgtaggt cgcggcatgt cttttcgtcg gcttggagga
     2401 tacctatcga ctgctgcgcg gcgagatgac cgccgaacag gccgagcagt tctaccgatc
     2461 ggcgtggccg ctgggcacca cgcttcaggt caccgaagac cagtggccgc ccacccgcgc
     2521 ggacttcgat gcgtactggc tggaggcctg caagcgcgtt caaatcgacg aacaagtccg
     2581 tgcgcatctg cacgatctgc tgaaccttcg catgatcaat ccggttctgg cgctgccgtt
     2641 tcggccgctg ctgaagttcc tcaccgtcgg attcttggcg ccggtgttcc gggaagcatg
     2701 ggcgtgcagt ggagtggttt caaacagcgc cagttcgagc ggctgtttct cctcgtcgcg
     2761 ttcgtcaacc ggttcctgcc cgtcttcatc cgccagggcg gcagctatgt actcctggtc
     2821 gacgtgcgcc gcagggcccg cgatcaccga gcgctggtgt gatgtcgtgt tactgcgtcg
     2881 agcgttgagc cgtcgggtca gtgtcgaggc gatgatcgaa ttcgcgctgt ggatggcgat
     2941 tccgtacatc gtcatcgggc tggcgttcgc gttcttcggc gccgagcagg tgcagctgat
     3001 cgagacgcag ct
//
LOCUS       DASRJA010000466        10334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_185399,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000466 DASRJA010000000
VERSION     DASRJA010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10334
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185399"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /gene="dxr"
                     /locus_tag="VFC01_20525"
     CDS             <1..895
                     /gene="dxr"
                     /locus_tag="VFC01_20525"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZN82045.1"
                     /translation="DVVLNALVGALGLEPTLAALASGARLALANKESLVAGGPLVLKA
                     AKPRQIVPVDSEHSAMAQCLRGGTPDEVAKIVLTASGGPFRGWPAERLEDVTPEQAGA
                     HPTWSMGPMNTLNSASLVNKGLELIETHLLFGVPYDLIEVVVHPQSIVHSMVTFTDGS
                     TLAQASPPDMKLPIALALGWPSRVPAAALACDFSTASTWEFEPLDAEVFPAVDLAREA
                     GRKGGCLTAVYNAANEEAAAAFLAGRIRFPAIVRTIAEVLRAADQWAAEPATVEDVLD
                     AQHWARDRAKGAVSREVVTTK"
     gene            895..2121
                     /locus_tag="VFC01_20530"
     CDS             895..2121
                     /locus_tag="VFC01_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HZN82046.1"
                     /translation="MMWIIGVALFALFILVSVALHECGHMWAARATGMKVRRYFVGFG
                     PTLWSTRRPNKLGYTEYGVKAVPLGGFCDIAGMTAIDEIAPEDEKYAMYRQKTWKRVL
                     VLFAGPGMNFVIGLVLIYGMALIWGLPNLHPQPAAYVSETSCVAPELSKGQMGKCDGV
                     GPAAAAGIKSGDIIVKVGDKDVANPDEMVAAVRALTGPTQFVVERDGTKFTTMVDVTR
                     TQRWTSDAAQAPTTVAAVGISAAEFGAVKYEQFNPATAIPGTVAFTGDLAVEIGKSLA
                     KIPTKVGALVHSIGGGERDPETPISVVGASIIGGDAFEHGYFMAFWFFLAQLNFVLGA
                     INLVPLLPFDGGHIAIAVFEKIRNMIRKARSIDAAPPVNYLKLMPATYVILVVVIGYM
                     ALTVTADFVNPIRPFQ"
     gene            2131..3291
                     /gene="ispG"
                     /locus_tag="VFC01_20535"
                     /gene_synonym="gcpE"
     CDS             2131..3291
                     /gene="ispG"
                     /locus_tag="VFC01_20535"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZN82047.1"
                     /translation="MTSGPTIGLGMPAPPAPTLAPRRKTRQLMVGDVGVGSDHPVAVQ
                     SMTTTKTHDINSTLQQIAELTATGCDIVRVACPRQEDADALSEIAKHSQIPVIADIHF
                     QPKYIFAAIHAGCAAVRVNPGNIKEFDGRVAEVAKAAGAAGIPIRIGVNAGSLDKRFM
                     AKYGKATPEALVESALWEASLFEEHGFGDIKISVKHNDPVVMVAAYEQLAAQCDYPLH
                     LGVTEAGPAFQGTIKSAVAFGALLSRGIGDTIRVSLSAPPIEEVKVGTQILESLNLRP
                     RSLEIVSCPSCGRAQVDVYTLANEVSAGLEGMEVPLRVAVMGCVVNGPGEAREADLGV
                     ASGNGKGQIFVKGEVIKTVPEAQIVETLIEEALRIAEERGTDASGAPIVTVS"
     gene            3347..4201
                     /locus_tag="VFC01_20540"
     CDS             3347..4201
                     /locus_tag="VFC01_20540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN82048.1"
                     /translation="MSAPPLFRLVDERRVSVVRDAAAVRQVLGEDPVGSCMVASRVAD
                     HGVEPSAIGGELWTRRRADESLCYAGANLIPLRGELTDLNAFADKAMSAARRCSSLVG
                     RAEYVMPMWRRLEHAWGPARDVREHQPLMALSTVPQTPIDPAVRPVRVDELDAYLVAA
                     IDMFIGEVGIDPRIGDGGRGYRRRVAGLIAAGRAWARFERGQVVFKAEVGSQSPAVGQ
                     IQGVWVHPDWRGRGLGTAGTATLASAVVRGGRIASLYVNDFNTVARATYARIGFTQIG
                     TFATVLLD"
     gene            4213..4872
                     /locus_tag="VFC01_20545"
     CDS             4213..4872
                     /locus_tag="VFC01_20545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN82049.1"
                     /translation="MTEQDNTVERREIARAMVRALDRRHELLDAVVASEDYDAAIEAI
                     ASLIGTSLVAAEAVLRLSFDRLTRVSRDRIAAELEDLNSQLSFISEQPVSTAQNLVLR
                     PFDADEDRDIFATRTDDVRSAGDGSGAPAGALDDEIRAAVSRVDAEEAVWLVAIDGSQ
                     KVGMVFGELAGGEVNVRIWIHPAHRKRGYGTAALRKSRSELAAYFPAVPLVVRAPGAG
                     G"
     gene            4928..6736
                     /locus_tag="VFC01_20550"
     CDS             4928..6736
                     /locus_tag="VFC01_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZN82050.1"
                     /translation="MATFVSRVVATLTVAAVVATLNACTPKPSGPQPTAEKFFAALAT
                     GDTAAAAELSDKPADARAALNEAWAGLQAVRLDAQILGSKYAEDTGSVTYRYTWHLPK
                     NRTWTYDGQLNMVRDEGKWEVRWSATGLHPRLGEHQTFALRADPPRRASVNERGGTDV
                     LVPGNLYHYALDAKAAGGALMTTARAVVGALWPFDNTLDPQRLAEQASSSKDPLSLIT
                     LRQSDHDRVAGAIGALPGVVVTPQAELLPTDDSFAPAIVGQVKKAVVDELDGEAGWRV
                     VSVNQNGVDVDVLNEVPGAPAPSVTISLDRSVQNAAQNAVNNSTGKKAMIVVIKPSTG
                     EVLAVAQNAAADSEGPLATAGLYPPGSTFKIITAGAAIDRDMATPNTLLACPGVMDIG
                     HRTIPNYGGFDLGTVPMSRAFASSCNTTFAELASRMPPRGLTMAAAQYGIGPDYQIEG
                     LNTVSGSVPPTVNLAERTEDGFGQGKVLVSPFGMALAAATVAAGKTPVPQLIEGRETT
                     ITGDRTPITPKMLDGLRPMMRLVVTNGTAKDLDGAGDVRGKTGEAEFEGGSHSWFAGY
                     RGDMAFAALIVGGGSSEYAVRMLKGMFDQLPPDYLA"
     gene            6733..7077
                     /locus_tag="VFC01_20555"
     CDS             6733..7077
                     /locus_tag="VFC01_20555"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN82051.1"
                     /translation="MSRWCISNRLKTVGVALVSILAATILLWAPPASADPTSNPEVAY
                     CQDMAALGHLADCAALASYGRGVCAQFDKGYDWYTILTRLDTITKDQQFSADILVAAV
                     SDICPWNESKKP"
     gene            7103..7684
                     /locus_tag="VFC01_20560"
     CDS             7103..7684
                     /locus_tag="VFC01_20560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HZN82052.1"
                     /translation="MADINHEYAMRVSDADRNGTLRRLHNAVALGLIDIDEFEERSAL
                     VSQARLHSDLDALVFDLPGPGAVVTSATDRVELRGVLGSLKRHGEWIVPTRLALHRRM
                     GSVDLDLTRARFAGPIVVIELDLKFGGLDLRLPDGASASIDDVEVNVGSAHDHRKEAP
                     AEGRPHVVLTGKVVCGSVDIRGPRRSWRFGRFT"
     gene            complement(7677..8840)
                     /locus_tag="VFC01_20565"
     CDS             complement(7677..8840)
                     /locus_tag="VFC01_20565"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZN82053.1"
                     /translation="MIADRFQPDRLVEQACESVGSDDFGADDTWRDGLGRLCEGFVSE
                     ARLNDLGVEIAVMDIVRALTSRLQIVKWRNENPAVAAQPITRPIFIVGQPRTGTTILF
                     DLLAQDPALRPPLTWEVDNPFPLPRPETYGTDPRIAETQASIEMSEQIVPGLLTWHPM
                     GALVGQECVRITAGQFCSMIFSVQYRLPSYYRWLLYEADHHPAYSYHRMFLQHLQSGV
                     PGQWLLKSPAHLWQLDALVAEYPDALIVQTHRDPLNVISSISALTHHLRHLASDESSI
                     TECAAQSREEIVVGLERGMKLRDSLSQRVVDVQFADFIRDPFATIRTLYAELGRELEP
                     VAEQNMRAFLAAHPGDGGGGRYSWADTGLDAGLVREEVRAYQERYGVPDEPLK"
     gene            complement(8837..9931)
                     /locus_tag="VFC01_20570"
     CDS             complement(8837..9931)
                     /locus_tag="VFC01_20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HZN82054.1"
                     /translation="METIGNDWGPKTLEAWRFVQQMLADMTQMVSDDALDERELLEGL
                     RVIAKVTGLCTELSVEADAERPRFFDMCSPTRLIGGPNPDGRYLLAMIRGDRTYRVTG
                     TRGGTAYLGFQVLAGTGLTPRRMAGYLSDTDLKGDSGSFTLVFSAVEPPADNLGAAQW
                     IPIPPEASSIVVREYVGDAGTEEPATMEIECLDAEPLQPVTDADAAEQFTAMAWTIVK
                     LTTLHRTVKPELLTQPNTLLTAEAAELGSADTTPDNLYMIGTFALEDDESLVLEFSPP
                     DTRYWNVTLESIWHECLEPKHRHSSVTNKGLRPDADGIARIAIGAKDFGHGHWLDTGG
                     RRRGFIVLRWLDNPEAPAVRTTVCRGEATP"
     gene            9951..>10334
                     /locus_tag="VFC01_20575"
     CDS             9951..>10334
                     /locus_tag="VFC01_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN82055.1"
                     /translation="MDVVSRPNSRESLTVRKQKATRARIAAAAAQSVAENGLSGATIE
                     QIAHAAEIGRATFFRYFSSKEDAVAEGLTTHWLDLITAAVARQPADLSASQAVVDAFE
                     DLGDGFDAISGQVRELAALTRSSPVL"
BASE COUNT         1727 a         3387 c         3536 g         1684 t
ORIGIN      
        1 ggacgtggtg ctcaacgcgc tggtcggagc gttgggcctg gagccgacgc tggccgcgct
       61 ggccagcggc gcccggctgg ccctggcgaa caaggagtcg ctggtcgctg gcggcccgct
      121 ggtgctcaag gccgccaagc cgcgccagat cgtgcccgtc gactccgaac actcagcgat
      181 ggcccagtgc ctgcgcggcg gcacccccga cgaggtggcc aagatcgtgc tgaccgcctc
      241 gggcggaccg ttccgcggct ggcctgccga acgcctcgag gacgtcaccc cggagcaggc
      301 cggcgcgcat cccacatggt ccatgggtcc gatgaacacg ctgaactccg cgtcgctggt
      361 caacaagggt ctggagctga tcgaaaccca tctgctgttc ggcgttccct acgacctcat
      421 cgaggtcgtc gtccacccgc agtcgatcgt gcactcgatg gtcaccttca ccgacggctc
      481 gacgctggcg caggccagcc cgccggacat gaagctgccc atcgcgctgg cgctgggctg
      541 gccgtcgcgc gtccccgcgg ccgccctggc ctgcgacttc tccaccgcct cgacgtggga
      601 attcgagcca ctcgacgccg aggtgttccc tgcggtggac ctggcccgcg aggcgggccg
      661 caagggcggc tgcctgaccg cggtctacaa cgccgccaac gaagaggccg ccgccgcctt
      721 cctggctggg cgcatccggt tcccggccat cgtgcgaacg attgccgagg tcctgcgcgc
      781 tgccgaccag tgggccgccg aacccgctac cgtggaagac gtacttgatg cgcaacactg
      841 ggcccgcgac cgggcaaagg gcgcagtttc ccgggaggtc gtcaccacca aatgatgatg
      901 tggatcatcg gcgtcgcgct gttcgcgctc ttcatcttgg tgtcggtggc gctgcacgaa
      961 tgcggacaca tgtgggcggc gcgcgcgacc gggatgaagg tgcgccgcta cttcgtcggc
     1021 ttcggcccga ccctgtggtc gacgcgccgg cccaacaagc tcggctacac cgaatacggc
     1081 gtcaaggcgg tcccgctcgg cgggttctgc gatatcgcgg gcatgaccgc gatcgacgag
     1141 atagcgcccg aggacgagaa gtacgcgatg taccggcaga agacctggaa gcgggtgctg
     1201 gtgctgttcg ccgggccggg catgaacttc gtcatcggtc tggtgctgat ctacgggatg
     1261 gcgctgatct ggggactgcc gaacctgcat ccacaacctg ccgcctacgt cagcgaaacc
     1321 tcttgcgtcg cacctgaact cagtaagggc cagatgggca agtgcgacgg cgtcgggccg
     1381 gctgctgcag cgggcatcaa gtccggcgac atcatcgtca aggtcggcga caaggacgtg
     1441 gcgaaccccg atgagatggt cgccgcggtg cgcgctctga ccgggccgac gcagttcgtg
     1501 gtggagcgcg acggaaccaa gttcaccacg atggtcgacg tcaccaggac gcagcggtgg
     1561 acctccgatg cggcccaggc gccgaccacc gtcgccgcgg ttgggatttc ggcggcggag
     1621 ttcggggcgg tcaagtacga gcagttcaac ccggcgacgg cgatacccgg gaccgtggcg
     1681 ttcaccggtg acctcgccgt cgaaatcggc aagtcgctgg cgaagatccc caccaaggtc
     1741 ggtgcgctgg tgcactccat cggtggcggc gaacgcgacc cagagacgcc gatcagcgtg
     1801 gtgggcgcat cgatcatcgg cggcgacgcg ttcgagcacg gctatttcat ggcgttctgg
     1861 ttcttcctgg cccagctgaa cttcgtgctc ggcgccatca acctggtgcc gctgttgccg
     1921 ttcgacggtg gccacatcgc gattgccgtg ttcgagaaga tccgcaatat gattcgcaag
     1981 gcgcgcagca tcgatgccgc gccgcccgtc aactacctca agctcatgcc ggccacctac
     2041 gtcatcttgg tcgtggtgat cggctacatg gcgctcacgg tgaccgctga cttcgtcaac
     2101 ccgatacgac ccttccagta ggagagaccg atgacctccg gccccacaat cgggctcggc
     2161 atgccggcac cccccgcacc gactttggcg ccgcggcgca agacccgcca gctgatggtc
     2221 ggcgacgtcg gcgtcggcag cgaccatccc gtcgcagtgc agtcgatgac caccaccaag
     2281 acgcatgaca tcaactcgac gctgcagcag atcgccgagc tgaccgcgac cggctgcgac
     2341 attgtccggg tggcctgccc gcggcaggag gacgccgatg cgttgtccga aattgccaag
     2401 cacagccaga tcccggtgat cgccgacatc cacttccagc ccaagtacat cttcgccgcg
     2461 atccacgccg gctgcgccgc ggtgcgcgta aaccccggca acatcaagga attcgacggc
     2521 cgggtcgccg aagtcgccaa ggccgctggc gccgcgggca tcccgatccg catcggcgtc
     2581 aacgccggct cgctcgacaa gcggttcatg gccaagtacg gcaaggccac ccccgaggcg
     2641 ctcgtcgaaa gtgcgctgtg ggaggcgtcg cttttcgagg agcacggctt cggcgacatc
     2701 aagatcagcg tcaagcacaa cgatccggtg gtcatggtcg ccgcctacga acagctggcc
     2761 gcacagtgcg actacccgct gcatctcggc gtcaccgagg cgggcccggc gttccagggc
     2821 accatcaagt ccgcggtcgc cttcggtgca ctgctgtcac gggggatcgg cgacaccatc
     2881 cgggtatcgc tgtcggcgcc gccgatcgaa gaggtcaaag tcggcaccca gatcctggag
     2941 tcgctgaacc tgcggccacg gtcgctggag atcgtgtcgt gcccgtcgtg tggccgcgca
     3001 caggtcgacg tctacacgct ggccaacgaa gtcagcgccg gcctggaagg catggaggtg
     3061 ccgctgcgcg ttgctgtgat gggctgtgtc gtcaacggtc ccggtgaggc gcgcgaggca
     3121 gacctcggag tcgcgtccgg aaacggcaag gggcagatct tcgtgaaggg cgaggtgatc
     3181 aagaccgtgc cggaggcgca aatcgtcgag acgttgatcg aagaggcact gcgaatcgct
     3241 gaagagcgcg gaacagatgc gagcggtgcg ccgattgtga ccgtaagctg agcatgaccc
     3301 tgtgagcggg gtcaccgcct cccgggatag gtcggaaaga gtctccatgt cggcaccgcc
     3361 gctgtttcgc ctcgtcgacg agcgacgggt atcggtggtg cgtgacgccg cggcggttcg
     3421 ccaagtgctc ggcgaggacc ccgtcggctc ctgcatggtg gcttcgcgtg tcgccgacca
     3481 cggcgtcgag ccctccgcga tcggcggtga gctgtggacg aggcggcggg ccgacgaatc
     3541 gctgtgctac gccggagcga acctcattcc actgcgcggc gagctcaccg atctaaacgc
     3601 gttcgcggac aaggcgatga gtgccgcgcg gcgctgttcg tcactggtcg gccgcgccga
     3661 atacgtgatg ccgatgtggc ggcgcctcga gcacgcctgg ggtccggcgc gcgatgtgcg
     3721 cgagcatcag ccgctgatgg ccttgagcac ggtgccgcaa accccgatcg acccggccgt
     3781 gcgccccgtt cgggtcgatg aactcgacgc ttatctggtc gcggccatcg acatgttcat
     3841 cggcgaggtt ggcatcgatc cgcgaatcgg cgacggcgga cgcggttacc ggcggcgggt
     3901 cgccgggctg atcgccgcgg gccgcgcgtg ggcccgcttc gaacgcggcc aggtggtctt
     3961 caaggccgag gtcggatcgc aatcgcccgc ggttggacag atccaggggg tgtgggtgca
     4021 tcccgattgg cgcggccgcg gcctcggtac cgccggcacc gcgacgctgg cgtcggcggt
     4081 ggtgcgcggc ggtcgtatcg cgagcttgta cgtcaacgac ttcaacacgg tggcccgcgc
     4141 cacctatgcg cgcatcggtt ttacccagat cggcacattc gcgaccgtcc ttctggacta
     4201 acgtctcgcg gtatgacgga gcaggacaac acggtcgaac gcagggagat cgcccgcgcg
     4261 atggtgcgcg cgctcgaccg ccggcatgaa cttctcgatg ctgtcgtcgc atcggaggac
     4321 tacgacgccg caatcgaggc gatcgccagc ctgatcggca cgtcgctggt cgccgccgaa
     4381 gcggtgctcc gattgtcgtt cgaccggctc accagggtgt cgcgggacag gatcgccgca
     4441 gaactcgagg atctgaacag tcagctgagt ttcatttccg agcaacccgt ttctacagcg
     4501 cagaacctcg tgctgcggcc gttcgacgcc gacgaggacc gcgacatctt cgccacgcgc
     4561 accgacgatg tgcgttcggc aggcgacggc tcgggggcgc ccgccggcgc cctcgacgac
     4621 gagatccgcg ccgcggtgag tcgagtcgac gccgaagagg cggtgtggct ggtggcgatc
     4681 gacggatcgc agaaggttgg catggtgttc ggcgagctgg cgggcggcga agtcaacgtc
     4741 cggatctgga ttcaccccgc ccaccgcaag cggggctacg gcacggccgc gctgcgcaag
     4801 tcgcgctcgg aattggcggc gtacttcccg gccgtgccgt tggtggttcg cgcgcctggg
     4861 gccggcgggt agtacacccg gtgcgcctgc gcgattatcg ggtctcaagt tcgtaacatc
     4921 tgccccaatg gcaacttttg tttcacgagt agtcgcgact ctgacggtcg ccgcagtggt
     4981 ggcgacgctc aacgcgtgca cgcccaaacc gagcggaccc caaccgaccg cggagaagtt
     5041 tttcgcagct ctcgcgacgg gtgacaccgc ggccgccgcc gaactcagcg acaagcccgc
     5101 cgacgccagg gccgccctca acgaggcgtg ggcggggttg caggcggtcc ggttggacgc
     5161 gcagatcctc ggctcgaagt acgccgagga caccggcagt gtcacctacc gatacacctg
     5221 gcatctgccg aagaaccgga cgtggaccta cgacggacaa ctcaacatgg tccgcgacga
     5281 gggaaagtgg gaggtgcggt ggagcgcgac cgggttgcat ccgcggctcg gcgagcatca
     5341 gaccttcgcg ctgcgggccg acccgccgcg gcgggcgtcg gtcaatgaac gcggcggcac
     5401 cgacgtgctg gtcccgggca acctttacca ctacgcgctg gatgcgaagg cggccggcgg
     5461 cgcattgatg acgacggccc gcgcggtcgt cggtgcgctt tggccgttcg acaacacgtt
     5521 ggatccgcag cggctcgccg agcaggccag ctcgtcgaag gatccgctga gcttgatcac
     5581 gctgcgccag tccgatcacg accgggtggc cggggccatc ggcgccctgc ccggcgtcgt
     5641 cgtcacgccg caggccgaac tgctgccgac cgacgattcg tttgcacctg cgatcgtcgg
     5701 ccaggtcaag aaggcggtgg tcgacgagct cgacggcgag gccgggtggc gggtggtcag
     5761 cgtcaaccag aacggcgtcg acgtcgacgt gctcaacgaa gtgcctggcg ccccggcgcc
     5821 gtcggtcacc atcagccttg atcgctcggt gcagaacgcg gcgcagaacg ccgtcaacaa
     5881 cagcaccggc aagaaggcga tgatcgtggt gatcaagccg tcgacgggcg aggtgctggc
     5941 cgttgcgcag aacgcggccg ccgacagcga gggcccgctg gccaccgccg gcctgtaccc
     6001 gccggggtcg acgttcaaga tcatcaccgc cggcgcggcc atcgaccgcg acatggccac
     6061 ccccaacacg ctgctggcct gtcccggtgt gatggacatc ggccaccgca ccattcccaa
     6121 ctacggcgga ttcgacctcg gcacggtgcc gatgtcgcgg gcgttcgcca gttcgtgcaa
     6181 caccaccttc gcggagctgg ccagcagaat gccgccgcgc gggctgacga tggccgccgc
     6241 gcagtacggg atcggcccgg actaccagat cgagggcctc aacacggtgt ccggctcggt
     6301 gccgccgacg gtgaacctgg ccgaacgcac cgaggacggt ttcggtcagg gcaaggtgtt
     6361 ggtcagcccg ttcgggatgg cattggccgc ggcgacggtg gcggccggga aaacgcctgt
     6421 gccccaactg attgagggcc gcgagacgac catcacggga gaccgcacac ccatcacgcc
     6481 gaagatgctc gacgggctgc ggccgatgat gcgtctggtg gtcaccaacg gcaccgccaa
     6541 ggatctcgac ggcgcgggtg acgtgcgcgg caagacgggt gaggccgaat tcgaaggcgg
     6601 ttcgcactcg tggttcgccg gctaccgtgg ggatatggca ttcgcggcgc tgatcgtcgg
     6661 gggagggtcg tcggagtacg cggtgcggat gctcaagggc atgttcgatc agctgccgcc
     6721 cgactacctg gcatgagcag atggtgcata tcaaacaggc tcaaaacagt tggggttgcg
     6781 cttgtttcaa tcctggcggc aaccatactg ctgtgggcac ccccggcgtc cgctgacccg
     6841 acatcgaacc cagaagtggc ctactgccaa gacatggccg ccctcgggca ccttgctgac
     6901 tgtgcggcgc tcgccagcta cgggcgggga gtgtgcgccc agttcgacaa gggttacgac
     6961 tggtacacga ttctgacgcg gctcgacacc atcacgaagg atcaacaatt ctccgccgat
     7021 atcttggtcg ccgctgttag cgacatatgc ccgtggaatg agtcgaagaa gccataagag
     7081 cagcggtacc gtgggtagcg gcatggcaga catcaaccac gagtacgcaa tgcgcgtgtc
     7141 agacgccgac cgcaacggaa cgctgcgccg gctgcacaac gctgtcgcgc tggggctgat
     7201 cgacatcgac gagttcgagg agcgctcggc gctggtgtcg caggcgcggc tgcactccga
     7261 cctcgacgcg ctcgtcttcg acctgcctgg accgggggcg gtcgtcacct cggccaccga
     7321 tcgggtggag ttgcgcggag tgctcggctc actcaagcgc cacggcgagt ggatcgtgcc
     7381 gacgcggctt gccctgcacc ggcggatggg gtccgtggac ctcgacctga cgcgggcacg
     7441 gttcgcagga ccgatcgtgg tcatcgagct ggacctgaag ttcggcggac tcgacctgag
     7501 gcttcccgac ggagcgagcg cttccatcga tgacgtcgag gtcaacgtcg gcagcgccca
     7561 cgaccaccgc aaggaagcac ctgccgaagg caggccgcac gtcgtcctca ccggcaaggt
     7621 ggtgtgcggc tcggtcgaca tccgcggccc ccgccgatcg tggcggttcg gccgctttac
     7681 ttgagcggct cgtcgggcac cccgtagcgc tcctgatacg ccctgacttc ctcgcgcacc
     7741 agccccgcat ccaggccggt gtccgcccac gagtatcggc cgccaccacc gtcacccgga
     7801 tgggccgcca agaacgcgcg catattctgc tcggcgacgg gttcgagctc acggccgagt
     7861 tcggcgtaca gcgtccggat ggtggcgaac ggatcgcgga tgaagtcggc gaactgcacg
     7921 tcgacgaccc gctgcgacag tgagtcgcgc agcttcatgc cgcgctcgag gccgacgacg
     7981 atctcctcac gcgactgggc ggcgcattcg gtgatcgagc tttcgtccga cgccaggtgc
     8041 cgaaggtggt gggtgagtgc gctgatcgac gagatgacgt tgagcggatc ccggtgcgtc
     8101 tgcacgatca gcgcgtcggg gtactcggcc accagcgcat cgagctgcca caggtgcgcc
     8161 ggcgacttca gcaaccactg acccggcaca ccagactgca agtgctgcag gaacattcga
     8221 tggtaggagt aggccgggtg atggtcggcc tcgtagagca gccaccggta gtagctgggt
     8281 agccggtatt gcaccgagaa gatcatgctg cagaactggc ccgcggtgat gcgcacacac
     8341 tcctgaccga ccaacgcacc catcggatgc cacgtcagca ggcccgggac gatctgctcg
     8401 gacatctcga tactggcctg tgtttcggcg atccgcgggt cggttccgta cgtctccggt
     8461 cgcggcaatg ggaagggatt gtcgacctcc cacgtcagcg gtggccgaag cgcggggtcc
     8521 tgggcgagca ggtcgaacag gatcgtcgtt ccggtccggg gctgtcccac gatgaagatc
     8581 ggccgggtga tcggttgggc tgcgacggcg ggattctcgt tgcgccactt gacgatctgc
     8641 agtcggctag tcagtgcgcg gacgatgtcc ataacggcga tctcgacgcc gagatcgttc
     8701 agccgcgcct cggagacgaa gccctcgcac agccgtccca gcccatcgcg ccacgtgtcg
     8761 tcggcaccga agtcatcgct gcctaccgac tcgcatgcct gctcgacgag ccggtcgggt
     8821 tgaaatcggt cggctatcac ggcgtcgcct cgccgcgaca gaccgtcgtg cgcacggctg
     8881 gcgcttcggg gttatcgagc caccgcagca cgatgaagcc gcggcgccga ccacccgtgt
     8941 cgagccaatg gccgtgcccg aagtccttgg cgccgatagc gattcgggca atgccgtcgg
     9001 catcgggccg gagccccttg ttggtcaccg agctgtgccg atgtttcggc tcgaggcatt
     9061 catgccagat gctctcgaga gtgacgttcc agtaccgcgt gtcgggcggg ctgaactcca
     9121 gcaccaacga ttcgtcgtcc tccaacgcga aggtcccgat catgtacagg ttgtcgggtg
     9181 tggtgtcggc actgcccaat tcggccgcct ccgcggtcaa cagcgtgttg ggctgggtca
     9241 gcagctcggg tttgacggtg cgatgcagcg tggtcagctt gacgatcgtc cacgccatcg
     9301 cggtgaattg ttcggcggcg tcggcatcgg ttaccggttg gagtggctcg gcgtcgaggc
     9361 attcgatttc catggtcgcg ggttcctcgg tacccgcgtc gccgacgtat tcccgcacga
     9421 cgatcgaaga ggcttcgggc gggatgggaa tccattgagc cgcaccgaga ttgtccgccg
     9481 gcggctcgac ggccgagaag acgagcgtga acgagccgga gtcgcccttg aggtcggtgt
     9541 cgctgagata gccggccatg cggcgcggtg tcaggcccgt gcctgcgagc acctggaagc
     9601 cgagataggc ggtgccgccg cgggttccgg tgacccggta cgtgcggtcg ccgcggatca
     9661 tggccagcag atatctgccg tcggggttgg ggccgccgat cagccgggtg ggcgagcaca
     9721 tgtcgaagaa gcgcggacgt tccgcgtcgg cctcgaccga cagctcggtg cacaggccgg
     9781 tcaccttggc gatgacgcgc aggccctcca gcagttcgcg ctcgtcgagg gcgtcgtcgg
     9841 acaccatctg cgtcatgtcg gccagcatct gctggacgaa ccgccaggct tcgagcgttt
     9901 tcgggcccca atcgttgcca atcgtctcca tggtgagaca tagtctcatc atggacgtcg
     9961 tatcacggcc gaattcgcgg gaaagcctga ccgtccgcaa gcagaaggcc acccgcgccc
    10021 gcatcgcagc ggcggcggcc cagtctgtcg ccgagaatgg cctttcaggc gcgacgatcg
    10081 aacagattgc ccacgccgcg gagatcggac gcgcgacgtt cttccggtac ttcagctcca
    10141 aggaagacgc cgtcgccgag ggcctgacaa cgcattggct tgacctgatc accgccgctg
    10201 tcgcccggca gccggccgac ctgtcagcca gtcaggccgt ggtcgacgcg tttgaagatc
    10261 tcggcgacgg gttcgacgcg atcagcggcc aggtacgcga gcttgcggcg ttgacgcggt
    10321 cgtcgcctgt gctc
//
LOCUS       DASRJA010000467         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1432979,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000467 DASRJA010000000
VERSION     DASRJA010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432979"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFC01_20580"
     CDS             <1..696
                     /locus_tag="VFC01_20580"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZN82056.1"
                     /translation="GGSMHLTKADKGMLGSYAIVGSHLPIAAGAAWSAKLRGTEQVAV
                     AFFGDGATNIGAFHESLNLAAVWKLPVLFVCENNLYMEYTPIASVTSVPNPAADRAVA
                     YGLPAEIVDGNDVVAVHDTMRDAVRRARAGEGPTVIEAQTYRHFGHSRADPGKYRPAD
                     EVERWMQRDPLRIARDRLTSLGVDEAEIDAADARVEKVVEAAVQEARDAPYPDPAQAL
                     TDVWADGGAAWRN"
     gene            684..1664
                     /locus_tag="VFC01_20585"
     CDS             684..1664
                     /locus_tag="VFC01_20585"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011600305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZN82057.1"
                     /translation="MAELITYRQAVSEGIAREMRRDSRVVCLGEDIGVAEGVFKTTTG
                     LFAEFGPERVWDTPISEQAIVGAALGAAMTGIRPVAEIMFSDFFACCYDYLANEIPKM
                     RYMTGGQVTVPLVIRTANGGGLGFGAQHSQAVENWVFTVPGLKIAAPSTPADVVGMMA
                     SAIRSDDPVIFFEHKGLFASKGEAAPPEHVVPLGEATVVRPGDDVTIVALASTVSTAL
                     SAAEQLAGEDISAEVIDLRGLVPLDAKTVLSSVARTSRLLLVEENPYQGGWGATVASI
                     VADEGFELLDAPIRRVAAECVPLPFADALEDEVIPTVNKVVDAVRRLAAF"
     gene            1710..>2844
                     /locus_tag="VFC01_20590"
     CDS             1710..>2844
                     /locus_tag="VFC01_20590"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN82058.1"
                     /translation="MTTKTLIRGGTVVSMDPQIGDLTADVLVEDDKIVAVEPNISADA
                     EVIDASDSIVIPGFVDTHRHTWEAAIRGCAPNATLDDYFVEVLDTFAPVYRAEDVHAS
                     NVAGSLECLNAGITTLVDWSHINNTPDHSDAAITGLQETGIRAQYAYGSANLSLADYW
                     FNSKIAIPGDDVRRVRDTYFSSDDGLLTLGLATRGTGFCMEEVVRAEWKLARELGIPI
                     TVHVAMGRLAGRFAMIKTLDDYGLLGPDTTYIHCCHFSDEEWQLVKDSGGKISIAPQV
                     ELSMGHGWPPMLHSLRMGLRPSLSIDVVTTAPGDMFTEMRAAFGADRARVHAEKYEPN
                     EQVPKDVLTAREVLQAATLDGAYVAGVEDRTGSLTPGKQADIVI"
BASE COUNT          526 a          886 c          934 g          498 t
ORIGIN      
        1 ggcggttcga tgcacctgac caaagccgac aagggaatgc tcggctcgta cgcgattgtc
       61 gggtcgcacc tgccgatagc tgccggcgcc gcttggtcag ccaaattgcg cggcacggag
      121 caggttgcgg ttgctttttt cggcgatggt gccaccaaca tcggcgcctt tcatgagtcg
      181 ctgaacctgg ccgcggtctg gaagctcccg gtgctgttcg tctgcgagaa caacctctac
      241 atggaataca ccccgatcgc ctcggtgaca tcggttccca atcccgcggc ggatcgagct
      301 gttgcatacg gactgcccgc cgagatcgtc gacggaaatg acgtggtcgc agtccacgac
      361 acgatgcggg atgccgtccg ccgggcacgc gcgggcgagg ggcccaccgt gatcgaggcc
      421 cagacctatc gccacttcgg gcatagccgc gccgatcctg ggaagtaccg gccggctgat
      481 gaggtcgagc gctggatgca gcgcgacccg ctgcgcattg cgcgtgatcg gctgaccagc
      541 ctgggagttg acgaagctga aatcgacgcg gccgatgccc gagtggagaa ggttgtcgag
      601 gcggccgtgc aagaggccag ggatgcgccc taccccgacc cggcgcaggc actcaccgat
      661 gtatgggcgg acggtggtgc ggcatggcgg aattgatcac ctatcgccag gccgtgtccg
      721 aggggatcgc ccgagagatg cgtcgcgact cgcgtgtggt ctgtctcggc gaggacattg
      781 gtgtggccga gggggtcttc aagaccacga ccggcctctt tgccgagttc ggtccggagc
      841 gggtgtggga cacgccgatc tccgagcagg cgatcgtcgg tgccgcgctg ggggccgcga
      901 tgacgggaat ccgcccggtc gcagagatca tgttctccga cttcttcgca tgctgctacg
      961 actacctcgc caacgagatc cccaagatgc gctatatgac aggcggccag gtcaccgtcc
     1021 cgctggtgat tcgcaccgcg aacggcggtg gtctcgggtt cggcgcacag cactctcagg
     1081 ccgtagagaa ctgggtcttc acggtgcctg gtctgaagat cgccgcacca tccactcctg
     1141 cagatgtcgt tggcatgatg gcatccgcga ttcgcagcga cgaccccgtg atcttcttcg
     1201 agcacaaggg tttgttcgcc agcaagggag aggccgcacc accggagcat gttgtgccgc
     1261 tgggtgaggc cacagtggtt cgtcccggcg acgacgtcac gatcgtcgca ctcgcctcaa
     1321 cggtgtcgac tgcattgtcg gcggcagagc agctggccgg cgaggacatc tctgccgagg
     1381 taatcgacct gcgcggtttg gtgccgctcg atgcgaaaac tgtgctgtcg tcggtggcgc
     1441 gcacctcgcg cctgctgctg gtagaagaaa acccctatca gggcgggtgg ggcgccacgg
     1501 tcgcttcgat cgtcgctgat gagggattcg aattactcga cgcgccgatc cgccgcgtgg
     1561 ccgcggagtg tgtacccctt ccgttcgccg acgcgctcga ggacgaggtg ataccgaccg
     1621 tcaacaaggt cgtcgacgcc gtacgacgac tcgccgcttt ctaggcaaac caaaagaaac
     1681 catcgccaaa tgcaaaggag ccggggcaga tgacaaccaa gacgctgatc cgcgggggga
     1741 cggtcgtgag catggatccc cagatcgggg acctgaccgc cgatgtgctc gtggaggacg
     1801 acaagatcgt cgccgtcgaa ccgaacatca gcgccgatgc cgaggtgatc gacgcgagtg
     1861 acagcatcgt catcccgggc ttcgtcgaca cccatcggca cacctgggag gccgcgatcc
     1921 gcggctgtgc gccaaacgcc acgctcgatg actacttcgt cgaggtcttg gacaccttcg
     1981 cgccggtcta ccgggccgag gacgtgcatg ccagcaatgt cgcgggatca ctggagtgcc
     2041 tcaacgccgg gatcacgaca ctggtcgatt ggtcacacat caacaacacg ccagatcact
     2101 ccgacgcggc catcaccggg ctgcaagaga ccggtatccg agcgcagtac gcgtacggca
     2161 gcgccaatct ctccctggcc gactattggt tcaacagcaa gatcgcgatt ccgggcgacg
     2221 acgtacgacg ggtacgggac acctacttca gctccgacga cggcctgctc acgttgggct
     2281 tggctacccg cggtacggga ttctgcatgg aggaggtcgt tcgggccgag tggaagctcg
     2341 cccgtgaatt aggtatcccg atcactgtgc atgtcgcgat gggccggctt gccggacgct
     2401 ttgccatgat caagaccctc gacgattacg gacttctcgg gcccgacacg acctacatcc
     2461 actgctgtca tttcagcgat gaggaatggc agctcgtcaa ggacagtggt ggcaagatct
     2521 caattgcgcc gcaggtcgag ctatcgatgg gtcacggctg gccaccaatg ctgcattcgc
     2581 tgcgcatggg gctgcggccc agcctgtcca tcgacgtggt gacgacagca ccgggcgaca
     2641 tgttcaccga gatgcgcgcc gcgttcggtg ccgatcgcgc ccgggtgcat gccgagaagt
     2701 acgagccgaa tgagcaggta cccaaggacg tgctgaccgc gcgggaggtc ctgcaggccg
     2761 caaccctcga cggcgcgtac gtcgccggcg ttgaggatcg caccggctcg ctgacacctg
     2821 ggaagcaggc cgacatcgtg atca
//
LOCUS       DASRJA010000468        29867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2815395,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000468 DASRJA010000000
VERSION     DASRJA010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 29867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29867
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2815395"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFC01_20595"
     CDS             complement(<1..244)
                     /locus_tag="VFC01_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN82059.1"
                     /translation="MVRVPLSPDQVKAGQRLGALIRTARAGRDPETIARDAGISPETL
                     RKIEVGRIPSPSFGTVIGLCNALGMPLQNAVDAWHGT"
     gene            325..1113
                     /gene="map"
                     /locus_tag="VFC01_20600"
     CDS             325..1113
                     /gene="map"
                     /locus_tag="VFC01_20600"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006896300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZN82060.1"
                     /translation="MIELKTAREIDKMAVTGEFVAETLATLSIEAQPGVNLMQLEHHA
                     RTLITDRGAKSCYWDYAPSFGRGPFRNVICLSVNDAVLHGLPRDYVLRDGDVLTLDFA
                     VSIDGWVADSAVTVIVGDRADAADRALVESTQRALDAGIAAAVPGGRLGDISAAIGEV
                     ATDAGYRVNAEFGGHGLGRTMHEDPHIPNRGRKGRGLLLRPGMTFALEPWWARGSDRL
                     VIDADGWTLRSADGSNTAHSEHTIAITETGPRILTPARCAHEAT"
     gene            complement(1150..2502)
                     /locus_tag="VFC01_20605"
     CDS             complement(1150..2502)
                     /locus_tag="VFC01_20605"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HZN82061.1"
                     /translation="MAVSVFDLFSIGIGPSSSHTVGPMRAAGRFARHLADDGLLPSVA
                     RVRVELYGSLGATGVGHGTPGAVVLGLEGSEPEAVDPIVAKGRVEEINTGGKLQLAGS
                     HTIQFDHSADIVLSLRAMDFHSNGMVYSAFDADDAEVSRRVYYSVGGGFVVDEHEAKS
                     GPREDAAVPYPFNTAEELVRLATDNGCSIAELMARNESALGRQADVRAGLLRIWAAMR
                     ACVDAGLAATGTLPGKLRVKRRARLLADTLDPDDPLYAMDWLTVYALAVNEENAAGGR
                     VVTAPTNGAAGVIPAVLHYYWRFVPGASDDGIVEFLLTAGAIGQLFKANASISGAEVG
                     CQGEVGSACSMAAAGLAAVLGGTPAQVENAAEIGIEHNLGLTCDPVGGLVQIPCIERN
                     AVASVKAITAARMALHGDGTHHVSLDTAIKTMRDTGADMKDKYKETSKGGLALNVVEC
                     "
     gene            complement(2520..2942)
                     /gene="cwsA"
                     /locus_tag="VFC01_20610"
     CDS             complement(2520..2942)
                     /gene="cwsA"
                     /locus_tag="VFC01_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthesis protein CwsA"
                     /protein_id="HZN82062.1"
                     /translation="MSSKTDVRLTPSQRLGRGLKYTAVGPVDVTRGTVGLSLQGAQSA
                     ADRIRRSIDKGRLAKELEGAQESIAQELAAAQEVLANLPQVIADARKPKRRRRPLLLA
                     AVAVVTLAGGAVAFSIVRRSMQPEPSPLPPSVDVAPKP"
     gene            3025..3570
                     /locus_tag="VFC01_20615"
     CDS             3025..3570
                     /locus_tag="VFC01_20615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZN82063.1"
                     /translation="MAHCHPVTSPIQTATATLHTNRGDIKIALFGNHAPKTVGNFVGL
                     AQGTKDYSTENASGSSSGPFYDGAVFHRVIDGFMIQGGDPTGTGRGGPGYQFADEFHP
                     ELQFDKPYLLAMANAGPGTNGSQFFITVGKTPHLNRKHTIFGEVLDPESQKVVDAIAT
                     TAVDRSDRPTDPVVIESITVS"
     gene            complement(3581..4000)
                     /locus_tag="VFC01_20620"
     CDS             complement(3581..4000)
                     /locus_tag="VFC01_20620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZN82064.1"
                     /translation="MQQTEWSPPALGIAACGIFGLILAIATVTLITDAPGRILMGIAA
                     TGLLVFASLSWRARPKLAISDDGLITRGWSRTNLLRRDDIKIIRITEFRRLARKVRLL
                     EIDTTDDRLLVFTRWDLGTDPLNVLDALTAAGYAGSP"
     gene            complement(4156..4419)
                     /gene="crgA"
                     /locus_tag="VFC01_20625"
     CDS             complement(4156..4419)
                     /gene="crgA"
                     /locus_tag="VFC01_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005082859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HZN82065.1"
                     /translation="MPKSKVRKKNDFTINPVSRTPVKVKSGPSSVWFVVLFCSLMLIG
                     LAWLLVFQLASNAIPWLAELGPWNYAIAFAFMITGLLLTMRWR"
     gene            4517..5266
                     /locus_tag="VFC01_20630"
     CDS             4517..5266
                     /locus_tag="VFC01_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HZN82066.1"
                     /translation="MEPSRRSAWRFGVPVVCLLAGLLLAATHGVSGGSEIRRSDAPRL
                     VDLVREAQQSVDRLTAQRDSLVSTVDNHHGGSPGADAALAAITNRSDKLAVDAGLDPM
                     RGPGLVVTLNDAQRDAEGRFPRDASPDDLVVHQQDIQAVLNALWSAGAEGVQMQDQRI
                     IATSAPRCVGNTLLLNGRTYSPPYVITAIGDVAAMQAALAAAPLVILYKQYVVRFGLG
                     YTEEPRPQVDLVGHTEPVRMKFAKPAGPLGY"
     gene            5361..6035
                     /locus_tag="VFC01_20635"
     CDS             5361..6035
                     /locus_tag="VFC01_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZN82067.1"
                     /translation="MQVLVVDNYDSFVFNLVQYLGQLGVHAHVWRNDDDRLATDADIA
                     KAAEQFDGVLLSPGPGTPERAGASIPLVNACAAAKTPLLGVCLGHQAIGVAFGGTVDR
                     APELLHGKTSTVFHSNAGVLQGLPDPFTATRYHSLTILPDTVPAELEVIARTEGGVIM
                     GVRHAELPIHGVQFHPESILTEGGHRMLANWLGFCGAAPDETLVRQLEDEVANAVQAA
                     TTRTPA"
     gene            complement(6013..7896)
                     /gene="pknB"
                     /locus_tag="VFC01_20640"
     CDS             complement(6013..7896)
                     /gene="pknB"
                     /locus_tag="VFC01_20640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HZN82068.1"
                     /translation="MTTPQHLSDRYELGEILGFGGMSEVHLARDLRLHRDVAIKVLRA
                     DLARDPSFYLRFRREAQNAAALNHPAIVAVYDTGEAETATGPLPYIVMEYVDGVTLRD
                     IVHTEGPMPAKRAIEVIADACQALNFSHQHGIIHRDVKPANIMISKNGAVKVMDFGIA
                     RALADANSVTQTAAVIGTAQYLSPEQARGEKVDARSDVYSLGCVLYEILTGEPPFIGD
                     SPVAVAYQHVREDPTPPSQRHNDISPELDAVVLKALAKNPDNRYQTAAEMRADLIRVH
                     SGETPDAPKVLTDAERTSLMSSSRPPSDRTEPIDQVGRPQAAYDDKDERSGSVGRWLI
                     AVAVLAVLTVVVTVAINMFGGSTRDVQVPDVKGKLENDAIAILQNAGFKTTTERNPDS
                     TIPPNKVIGTDPSAGTSVGAGDEITLNVSTGPEQREVVDCKGVSYADCVQKLSNAGFG
                     KFRASPSPSTPEMKDKVLATIPPANQTSAITNEITIVVGSGPESKPVPDVKNQTVESA
                     QSVLSASGFTKPVVVEVDSPSPKGTVMGTLPPAGDIVPLDSVIQVQVSMGNQFVMPDV
                     RGGFWTDVEPNLRNAYGWTGPLIRGPDVQNSGQRTNAVVTQSPAAGTPVKFSDPITLA
                     FAS"
     gene            complement(7893..9155)
                     /locus_tag="VFC01_20645"
     CDS             complement(7893..9155)
                     /locus_tag="VFC01_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN82069.1"
                     /translation="MSPRVGVTLSGRYRLQRLIATGGMGQVWEGIDSRLGRRVAIKVL
                     KAEYSTDAEFVERFRAEARTVAMLNHPGIAGVYDYGETEMDGEGRTAYLVMELVNGEP
                     LNSVLKRTGRLSLRHALDMLEQTGRALQVAHTAGLVHRDVKPGNILITPTGQVKLTDF
                     GIAKAVDAAPVTQTGMVMGTAQYIAPEQALGHDATAASDVYSLGVVGYESVSGKRPFT
                     GDGALTVAMKHIKETPPPLPADLPPNVRELIEITLVKNPGMRYRSGGAFADAVAAVRS
                     GRRPPRPNQAPSIGRATPAAVPSAAQARAAADLTGRAPATAARARPATGSHRTPPPRR
                     TFSSGQRALLWAAGVLGALAIVIAILIVLNAQDRKDQQSPSPTVTNTITETTPFQSPS
                     AMPGWTDEGTFRHGGLEIDAIAPEQTWK"
     gene            complement(9355..10830)
                     /gene="pbpA"
                     /locus_tag="VFC01_20650"
     CDS             complement(9355..10830)
                     /gene="pbpA"
                     /locus_tag="VFC01_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-transpeptidase PbpA"
                     /protein_id="HZN82070.1"
                     /translation="MNTSLRRIAVTIMVLIVLLLANATVTQVFTADGLRADPRNQRVL
                     LDEYSRQRGQISAGGQLLAYSVSTDGRFRFLRVYPNPLAYAPVTGFYSLSYSSTGLER
                     AEDTILNGSDQRLFGRRLADFFTGRDPRGGNVATTINPQVQQAAWDAMEDGCNGPCKG
                     AVVALEPSTGKVLAMVSSPSYDPNLLATHDIQAQSAAWQRLRDNPDSPLLNRAISETY
                     PPGSTFKVITTAAALQSGATVNTQLTAASRVTLPDSTATLENYGGASCGAGPTASLRD
                     AFAKSCNTAFVQLGLNTGADALRSTALAFGLDSPPPTIPLQVSESTVGPITDAAALGM
                     SSIGQKDVALTPLQNALVAATIANKGVTMQPYLVDSLKGPDLANISTTAPREERRAVS
                     EQVANTLTDLMVAAEQVTQQKGAIAGVQIASKTGTAEHGTDPRNTPPHAWYIAFAPAS
                     APKVAVAVVVENGGDRLSATGGAVAAPIGRATIAAALREGS"
     gene            complement(10827..12236)
                     /locus_tag="VFC01_20655"
     CDS             complement(10827..12236)
                     /locus_tag="VFC01_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZN82071.1"
                     /translation="MTTQPQSPVTVMPPLPNRRNAELFLLGFAALLTTVALLLVEANQ
                     EQGLHWDLAQYAVAYLALFTGAHLAIRRFTPYADPLLLPVVALLNGLGLVMIHRLDLA
                     AGTPMSKGLGGTANQQMLWTLVGVIGFSLVVIFLRDHRMLARYGYVCGLTGLVLLVIP
                     AVLPRSMSEQNGAKIWIRLPGFSIQPAEFSKILLLIFFAAVLVAKRSVFTSAGKHFLG
                     MDLPRPRDLAPLLAAWIASVAVMVFEKDLGTSLLLYASFLVLVYVATDRFSWVVIGLA
                     LFAAGSVAAYYVFDHVKVRVETWLDPFADPDGAGYQMVQSLFSFATGGIFGTGLGNGQ
                     PGTVPAASTDFIIAAIGEELGLVGLAAVLMLYTIVIIRGLRTAIAVRDSFGKLLAAGL
                     ASTLAIQLFIVVGGVTRLIPLTGLTTPWMSYGGSSLLANYVLLAILVRISHAARRPIV
                     TRPHSQPIAAAGTEVIEKV"
     gene            complement(12367..13911)
                     /locus_tag="VFC01_20660"
     CDS             complement(12367..13911)
                     /locus_tag="VFC01_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HZN82072.1"
                     /translation="MTLVLRYAARSDRGLVRANNEDSVYAGARLLALADGMGGHAAGE
                     VASQLVIAALAHLDDDEPGGDLLSKLDAAVREGNSAIAAHVEADPELEGMGTTLTAIL
                     FAGNRLGLVHIGDSRGYLLRDGELAQITKDDTFVQTLVDEGRITAEEAHSHPQRSLIM
                     RALTGHEVEPTLIMREARAGDRYLLCSDGLSDPVSHETILEALQISDVAESADRLIEL
                     ALRGGGPDNVTVVVADVIDYDYGQTQPILAGAVSGDDDQVAPPDTAAGRASAFNPRRN
                     EAKRVVPQPEEPQRKPRSRRRMLIAAVLLVLVVLAGLAVGREIVRSNYYVTEHDGTVS
                     IMRGVQGSFLGLALQEPYLLGCLNARNELSLISAGQSKNNSGCRMLGVDDMRPSERAQ
                     VVAGLPSGTLDDAIAQIEELARSSVLPVCATRTPTTTTTSPTPHLPQTPVNPGIPETG
                     GDTPAPETPTTVTASAPAPPPGPPPPGAPPPSAPPPSPTPSPQPTVTALPPPPPEPGT
                     NCREVS"
     gene            complement(13908..14375)
                     /locus_tag="VFC01_20665"
     CDS             complement(13908..14375)
                     /locus_tag="VFC01_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN82073.1"
                     /translation="MQGLVLQLTRVGFLLLLWLFIWSVLRILRTDIYAPTGAVMVRRG
                     LALRGSLLPNRGRRHVARQLVVTEGALTGTRITLGTQPVLIGRADDSTLVLTDDYAST
                     RHARLSPRGSEWYVEDLGSTNGTYLDRAKVTTAVKVPMGTPVRIGKTVIELRP"
     gene            complement(14528..16036)
                     /locus_tag="VFC01_20670"
     CDS             complement(14528..16036)
                     /locus_tag="VFC01_20670"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HZN82074.1"
                     /translation="MARRHAGALVPEQDRRTTTSEAGGDMGLVDRFERKLESTVGDAF
                     ARVFGGSIVPQEVEAMLRREADTGAREVLGDRILAPNDYVITLSVPDYQKVSADPDLT
                     STTFAKHLEGYIHEQGWQTYGDVVVRFEASPNLHTGQFRARGAVNPDTTTGEPAAPRE
                     LSSNAEPGVPPMSDNPSYRGGGQGQGRPADEYYDDRYGRPQDERGQDPRGQYPPQEQQ
                     GGGYPPQAEQGYPPRQGYPDQAGYPDQGGYPDQGYPPPSYEQRPPAGYGPPAGGGYQD
                     QGYRQAPGGYPPPQGGQQGYGAPAGDYDYGRQPAPGRPDEGYGRAEPRPAYPDQGGYP
                     DQGGYPDQGGYGGQAYGRQDYAQPDYGRYGEAPAGAGYAEPGYGEPAGGGYDYGQPTG
                     QGYGGGYAEGGYPAGGTSVTLQLDDGSGRTYQLREGANVIGRGQDAQFRLPDTGVSRR
                     HLEIRWDGQVALLSDLNSTNGTTVNNAPVQEWQLADGDVIRLGHSEIIVRVH"
     gene            16220..16302
                     /locus_tag="VFC01_20675"
     tRNA            16220..16302
                     /locus_tag="VFC01_20675"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16253..16255,aa:Leu,seq:cag)
     gene            complement(16374..17132)
                     /locus_tag="VFC01_20680"
     CDS             complement(16374..17132)
                     /locus_tag="VFC01_20680"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN82075.1"
                     /translation="MDSKAAPPVGSVDKALALIELLAEAGPDGLALRDVVEASGLNKA
                     SAHRTLQALQHRGFAEQDANQRYRLGSRPALLVEQFLREENLPTLFRPALLAICQESQ
                     ELVHLGILEGPTVLYLDKVEPDRTIRVWSRVGRQVSVLTTALGRALIAAEGATTQLSA
                     YTANADPKVAKRFERAVAEARLHGFATEREENEAGICCVGVALRRSTGRPVAVSVTGP
                     SNRMGADRLDKLGRRLRDVLQLSAPPGFSVAPTG"
     gene            17292..18782
                     /locus_tag="VFC01_20685"
     CDS             17292..18782
                     /locus_tag="VFC01_20685"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HZN82076.1"
                     /translation="MRRLSRRTTPDGAMIGASAGAATGIVHLGLGNFHRAHAAVYTAN
                     ALAAEPGDWGIYGFANRSHDVVSAMRAQDGRYAILELSDRGRQVGIVDVHRGLGVLAD
                     DRTAFVAAVADPSQRILTLTVSEVGYCRSPRTGTLDVDRDDVRADIADPAHPRSTIGL
                     IARGLSVRSASGAPITVLSCDNLQSNGHSTRAALTEFLRASAASDDALNFLENHVSFP
                     NSMVDRIVPATTAQTNADVAELIGVADHAPVPTEDFSMWVLEDDFAAGRPAWDQAGAI
                     MSDEVEAYELVKLRLLNGSHSLIAYLGVLDGRATIADAWAQDFVQDAVRTCIAREYLP
                     TIALPEGFDPDSYLESLSHRWANAALGHRTSQVGTDGSVKLLQRIPEPAQIALRAGRV
                     PHLLALTVAGWICAAAPPTGFDPGPNAAEIREPARQRLAEVTQGAGGPREHAMAVLRN
                     GFLPDSLTAHAAFTDRVADLVEVIARNGVRAAAREAIEVDNDRKVS"
     gene            18779..19783
                     /locus_tag="VFC01_20690"
     CDS             18779..19783
                     /locus_tag="VFC01_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018340396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HZN82077.1"
                     /translation="MKALAIHGKEDIRWEDCAAPVPADGQVRLRVSYVGICGSDLHYY
                     FYGANGENVVREPFTPGHELSAVVDLDPSGRLPSGAPVTVHPARYGQPVPGLADRPHL
                     CPGGDYLGSAATFPHRQGGAAELLIVEDHMVRKLPEGLPLRRAALAEPLAVALHAVGL
                     AGDLTGRRVLVIGAGPIGLLVVAAALDRGATEIGVSDLRPEPLARAAALGATEPVLVG
                     RDSIADESFDVVFECSGVGVSLTQAVRAARRAGTVVQVGTLPNTEISVNLAPMLGKEL
                     RLLGAFRFATEIDDAIAMLAASDHLESVISHVIPAAEAVDAFTLARDASASAKVLLQL
                     "
     gene            19833..21227
                     /locus_tag="VFC01_20695"
     CDS             19833..21227
                     /locus_tag="VFC01_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN82078.1"
                     /translation="MTTTPEPRTAELPSTAQRSMADLVRAAVSGWLGTALEFMDFQLY
                     SLAAALVFTQLFFSGESAGMAVVWAMATYGVGYIARPLGAIFFGRLGDRIGRRKVLFY
                     TIALMGAATTLIGALPTYAQVGMLAPILLVSLRLLQGFGAGAEISGAGVMLAEYAPAQ
                     RRGFIASLVGLGTNCGTLFASAIWALLLGVMVESDVIAWGWRIPFIASAVVMVFAVWV
                     RLNLKESPVFEERDDVVDGRALSREQMIEQAEEHSDTRTLEAVERKPIKAVLVSFFLR
                     FGQAGNSGLIQTYLISFMTITLAMQKSVGANAVIVSSLVGFLTIPLVGWLGDRLGRRR
                     MYIVVMSAALVVIVPTMLAIDSKNLVWVFVGYIVLHNVAVLSPASLENVSIPEIFGAR
                     NRYTLTAIIREIAAVIATGIGPVVAAAWVAAATGSPIPIMVMLGLYSVAALIAAVRAK
                     DWTGRDLTDPRPAM"
     gene            21234..22475
                     /gene="manD"
                     /locus_tag="VFC01_20700"
     CDS             21234..22475
                     /gene="manD"
                     /locus_tag="VFC01_20700"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005388996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HZN82079.1"
                     /translation="MTIEKIDVNVCSPGRNFVTLKIVTADGVIGWGDATLNGRELSVA
                     AYLSEHLASTLIGRDEDRIEDAWQYLYRGAYWRRGPVTMAAIAAVDMALWDIKAKKAG
                     MPLYQLLGGASRDRVRVYGHASGGDYAALKDAISGYVDAGYTAVRVQTGVPGLGQIYG
                     VATNKPGTRYDYEPAHRAADGSSATAPIEETWDTRAYLTHMPGVFAKIREDFGTGLRI
                     LHDGHHRMTPIEAARFAKSVEPYDLFWLEDCTPGEDQAALRLVRQHSTTPLAIGEVFN
                     SVYDYQTLITERLIDYVRSAVTHTGGVTAMKKLLDFAGIYGIKSGMHGPTDISPVGMS
                     AALHLDLAIHNFGIQEYMPHNDTTLDVFKTSYTFDRGFLHPGEAPGLGVELDEQAAAA
                     FGYTAAYLPVNRLQDGTIHDW"
     gene            22465..23121
                     /locus_tag="VFC01_20705"
     CDS             22465..23121
                     /locus_tag="VFC01_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005572977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HZN82080.1"
                     /translation="MTGDTLARTQLPDRTATAKLIVVVRAADAADYDRVLDALIDAGI
                     RSIELTLTTPGTIDRLPKLLTQFGDTADIGIGTVTDVPQLTAAVDAGAHYLVTPISDP
                     ELVDAANRAGVPIVPGGLTPTELFAAWRAGAAAVKIFPANQVGPGYLNDLRGPFPGIA
                     AVPSGGVDLEGAAAWLRAGAAAVSVGGPLLGDALRGGDFGALRDRTTEFVATCGQVTT
                     "
     gene            23118..24068
                     /locus_tag="VFC01_20710"
     CDS             23118..24068
                     /locus_tag="VFC01_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HZN82081.1"
                     /translation="MTARVATVGETMALMRTTDIGSLRHVSDMVLGIGGAESNVAIGL
                     RRLGVDVTWLGRVGDDPLGERVTREIRAEGVDVRAVIDTQAPTGLMLKERPSASSTAV
                     FYYRAGSAGSRLQPDDMPPGWIENVELVHVSGITALLSDSARDCVLTAISRAQASGVQ
                     VSFDVNYRSTLAPADVAGPLLRKIAENADFVFGGPEELTLLYPDSEPAEAAQRLMRSG
                     RREVVLKKGADGAAVYSSDAVVESAGFTVDVVDTVGAGDAFVAGYLSGLLHGLDVPGR
                     LNRANACGAMLCMTPGDWESSPTLSDVERFCRPGTDPVLR"
     gene            24078..25262
                     /locus_tag="VFC01_20715"
     CDS             24078..25262
                     /locus_tag="VFC01_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82082.1"
                     /translation="MRLAYIAFVSVVLLLSAACSTAVQGSAVKESGARSGPTIDVSRL
                     DVGPYPTEPSQPLGVTGDPERGVLVEAQRMANNVVGPWEVDSSLTGWFSFGATVLPSP
                     DALAQIGPEPFAAAAAQHGFINGFASARTAEGQKILLNAVLRFDDTAAAAAAATEFGD
                     IAARTGEGVQRAQIPGHPDTQAASYTQTEDSTGKRWSAVRAFTPHGQYVFMQLAQAVD
                     GMDAAIGLVASTVDAQGPIIDKFRATDPSEFADISLDPTGLLARTLPVPEKEATTIQD
                     ATYEQRGALHFQSDPARSAKLFTDTEMDLVAMARTNVYETKDSASAVKIVDGFFAELQ
                     PTSQPAKPVNNLPDSRCLQLKDKSFYCLGAADRYAIETTSDKLLDAQQQVAAQYAMLL
                     SG"
     gene            complement(25259..25843)
                     /locus_tag="VFC01_20720"
     CDS             complement(25259..25843)
                     /locus_tag="VFC01_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN82083.1"
                     /translation="MERRHRRQGSRRDPSIDKAVLAAARRLLVDRGYAATTIDLIATT
                     AGVSRPAVYRRWSSKAQLVHEALFPDLGPEPPEDDFAAEITRLCRGATRMYGDAAVRE
                     SIPGLLNDLRSDRAMRRVLSDRLEATARSQLAQRVGEAVADGTARPGISADTLMDAIA
                     GGAWYAVCVRRIKDVDTAAKELSELVLRGVLAGG"
     gene            25907..28159
                     /locus_tag="VFC01_20725"
     CDS             25907..28159
                     /locus_tag="VFC01_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN82084.1"
                     /translation="MTTGLTNPLAGVGEDGRHLYTCPLCEAMCGLEIHVADGRVSTIR
                     GNRDDTWSHGHICPKGATLGAVHEDPDRIRRPLIKVDGQWQEVSWDAAFRRCTELLAP
                     VIEKHGIGAVTCYTGNPLAHSFSLGRYTGVLLGMSGIPLSYSPGTVDQWPKNLSSHLM
                     YGGWWTFPVPDIERTDLLIVMGANPAASQGSLLAAPDVMGIIDGIRKRGKVIVIDPVR
                     TATAARASEWLPIAPGTDAALLLAVVHTLFDENLVKLSDLEPHLDGVDRLREVAAGWA
                     PERVAAVTGITADRIRTLARELATTERAVVYGRIGLCNQEFGSLASWLVDVVNILTGH
                     FDSPGGSMFPRAAAWSVTVQPIPGLEDGAPEFGRYRTRVRGAKEVLGQVPVSCLAEEI
                     ATPGEGQIRALITVAGNPVLSTPAGHKLDEALPTLDAMIAVDLWLNETTRHADVILPA
                     PSPLEQPHHDDLILNFAINSIANYSPPVFAPEDPDRPEEWEILIRLTGLCTGMPAEDV
                     DVAAIDDGFFDYMAFTQGLEGAEIRKHYERGGPERILDFTLRTGPFGDRYGENPDGVT
                     LEKLKAEPNGINFGPMVPQVPEILGTADGKIRLAPQYLLDDLPRLAARLDRVPDELVL
                     VSRRHLRSNNSWLHNVGPLMKGRDRCTLLMHDDDATKRGVATGDLVTVTSSGGQIEVP
                     VEVTDAIMPGVVSMPHGWGHGKPGTRMSIANGSPGVNTNILSPPTFLDEPSGNGALNG
                     IPVTVTAAAN"
     gene            28172..28669
                     /locus_tag="VFC01_20730"
     CDS             28172..28669
                     /locus_tag="VFC01_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZN82085.1"
                     /translation="MGTNQRAKIVMTDDEIAEFIERSRTATMATVLPDGRPHLVAMWY
                     AVVDGEIWFETKAKSQKAVNLFRDPTITVMIEDGQSYDTLRGVSIDGKAEIVDDPETI
                     LRVGISVWERYTGPYTDEMRPFIDQMMNNRICVRVVPSRLRSWDHRKLGMAPMPIGGS
                     TAQYL"
     gene            28669..29145
                     /locus_tag="VFC01_20735"
     CDS             28669..29145
                     /locus_tag="VFC01_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN82086.1"
                     /translation="MPQERPKQLDSPLLPKIFKYAGKAHVWVYRRTGGKVGGKWRIGA
                     GFRKPVPTLLLEHRGRKSGKVFVTPLLYLLDGADVVIVASQGGRAEDPQWYRNLVANP
                     DTFVEIGADRRAVQAVTAGPEERARLWPKLVELYADFDNYQSWTDREIPVVVLKPR"
     gene            complement(29142..>29867)
                     /locus_tag="VFC01_20740"
     CDS             complement(29142..>29867)
                     /locus_tag="VFC01_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HZN82087.1"
                     /translation="ISGNYIGGILGHVLERDAGDPDFQPTRLTVDLFRPAALAPVRVD
                     TTITRQGRRLRLVDAVMTQDDTVVARASGLFLRRGEQPADEIWSSPLSMPPIPPTPDP
                     IPRGLAMLVWTYGRSDHTPGPGFGLDAWAHAGPKYIWVRDIRPLVDGEELTPFTRAAM
                     AGDMVSSLTHFGAEGLQFINADYTLTLSRLPQGPYLGLAALTHYSHDGVATGTATIVD
                     QHGPIGSGVANALSNPGFSPPHP"
BASE COUNT         4949 a         9922 c         9980 g         5016 t
ORIGIN      
        1 ctgtcccatg ccaggcgtcg acggcattct gcagaggcat gccgagcgcg ttgcagagtc
       61 caatcaccgt gccgaagctc gggctgggga ttcttccgac ctcgatcttg cgcagtgtct
      121 ccggcgagat gccggcgtca cgcgcgatcg tctcggggtc gcggccggcc cgggctgtcc
      181 tgatcagcgc gccgagacgt tgcccggctt tcacctggtc cgggctcaga ggtacacgca
      241 ccatgccggt cagcatagcc ggtatttaag taccggtact ggtaccgtcg ctacggtata
      301 aaaataccga tcgggaggcc cagcatgatc gaactcaaga ccgctcgaga gatcgacaag
      361 atggcggtga ctggtgagtt cgtcgcggaa acgctggcga cgctgtcgat cgaggcgcag
      421 cccggcgtga acctgatgca gctcgagcac catgcccgca cgctgattac tgaccgtggt
      481 gcaaaatcat gctactggga ttacgcgccg tcgttcggtc gcggtccatt ccggaatgtc
      541 atctgtctgt cggtcaacga cgctgtgctg catggccttc cacgcgacta cgtgctacgt
      601 gatggcgacg ttctgactct ggactttgcg gtgtcgatcg acggttgggt agccgattcc
      661 gcggtcacgg tcatcgtcgg tgacagggcc gacgccgctg acagagccct ggtcgagtcg
      721 acgcaaagag cccttgacgc cggtatcgcc gcagcggtcc ccggcggtcg tctcggtgac
      781 atctccgccg caatcggcga agtcgctacc gacgccgggt atcgcgtcaa cgccgaattc
      841 ggcgggcatg gattagggcg caccatgcac gaggatcccc acatccccaa tcgtggacgg
      901 aagggacgcg gattactgct gcggccgggc atgaccttcg cgcttgaacc gtggtgggca
      961 cgaggcagtg atcgtctggt tatcgatgcg gacggctgga cgctgcgctc ggcagacggg
     1021 tcgaacaccg cgcactccga gcacaccatc gccatcaccg aaaccggccc gcggatactc
     1081 actcctgcgc gatgcgcaca cgaggcgacg tgacgtgatg gaccgcaacg cgccgtcggc
     1141 attcgctaac tagcactcca ccacgttgag ggccagcccg cccttcgacg tttccttgta
     1201 cttgtccttc atgtcggcac cggtgtcgcg catcgtcttg atcgcggtgt cgaggctgac
     1261 gtgatgagtt ccgtccccgt gcaacgccat tcgcgctgcc gtgatcgcct tgaccgacgc
     1321 gaccgcgttg cgttcgatgc acggaatctg taccaggccg ccgaccgggt cgcaggtgag
     1381 cccgaggttg tgttcgatac cgatctccgc ggcgttctcc acttgcgccg gcgttccgcc
     1441 caacaccgcc gccaacccag cggccgccat cgagcacgcg gagccgacct caccttggca
     1501 gcccacttcg gcgccggaga tcgaagcgtt ggccttgaac agctgaccga tcgcaccggc
     1561 cgtcaacagg aattcaacga tgccgtcgtc gctcgcaccg gggacgaacc gccagtagta
     1621 gtgcagcaca gcgggaatga cgcccgcggc gccattcgtc ggcgccgtca cgacccggcc
     1681 gccggcggcg ttctcctcgt tgaccgccag cgcgtacacc gtcagccaat ccatcgcgta
     1741 caacggatcg tcgggatcca gcgtatccgc tagcaaacgc gcgcggcgtt tgacacgcag
     1801 cttcccgggc agcgtgccag tcgcggccag gccagcgtcg acgcatgcgc gcatcgccgc
     1861 ccagatccgc agcaggccgg cccgcacatc ggcctgacgg cccaaggcgg actcattgcg
     1921 tgccatcagt tcggcgatgc tgcagccgtt gtcggtggcc agccgcacga gctcctcggc
     1981 cgtgttgaac ggatacggaa cggcagcgtc ctcgcgcggg cccgacttcg cctcgtgctc
     2041 gtcgacgacg aacccgccgc cgaccgagta atacacacga cggctcactt cggcatcgtc
     2101 ggcatcgaat gccgagtaca ccatgccatt gctgtgaaag tccatggcgc gcaacgacaa
     2161 aacgatgtca gcggagtggt cgaactggat cgtgtggctg ccagccagct gcagcttgcc
     2221 gccggtgttg atctcctcga ccctgccctt cgcaacgatc gggtcgaccg cctctggttc
     2281 gctgccctcc agcccgagaa cgaccgcacc aggcgtgccg tgtccgacgc cggtggcgcc
     2341 cagcgagccg tatagctcga cgcgtacccg tgccacgctc ggcagcaatc cgtcatcggc
     2401 gagatgacgc gcgaaccgac ctgcggcgcg catcggaccg acggtatgcg aactcgacgg
     2461 ccctatgccg atcgaaaaga ggtcaaagac cgagacagcc atcgcgcacc tacagcaaat
     2521 tacggtttgg gagccacgtc gacgctgggc ggcagcggcg acggttcggg ttgcatcgac
     2581 cggcgcacga tgctgaacgc caccgctccc ccagccagcg tcaccacggc caccgcggcg
     2641 agcaggaggg gtcgacgcct gcgcttgggc ttgcgcgcgt cggcgatgac ctggggcagg
     2701 ttcgcgagca cctcctgggc cgccgcgagt tcctgcgcga tgctctcttg cgcgccctcg
     2761 agctccttgg ccagccggcc cttgtcgatg ctgcggcgaa tgcggtcggc ggctgactgc
     2821 gcgccctgca ggctgagccc gaccgtgcca cgcgtgacgt cgaccggtcc gaccgcggtg
     2881 tacttcaggc cgcggcccag ccgctggctc ggggtcaacc ggacgtccgt cttcgagctc
     2941 atgtccacct ctttcgcggc gtgatcgtca atccatatcc agcctgccat caagtggcac
     3001 cccgcggagg ccgttgggcg gcgggtggca cactgtcatc ccgtgacgag ccccattcag
     3061 acagcgaccg ccaccctgca caccaaccgc ggtgacatca agatcgcgct cttcggaaac
     3121 cacgccccca agaccgtcgg caatttcgtc ggactggcgc aggggacgaa ggactacagc
     3181 accgagaacg cgtcaggcag ctcgtcggga ccgttctacg acggcgcggt cttccaccgt
     3241 gtgatcgacg gcttcatgat ccagggcggc gacccgaccg gcaccggccg cggcggtccg
     3301 ggataccagt tcgccgacga gttccaccct gagctgcagt tcgacaagcc gtatctgctc
     3361 gcgatggcca acgcagggcc aggcaccaac ggttcgcagt tcttcatcac ggtcggcaag
     3421 acgccgcacc tgaaccggaa gcacacgatc ttcggcgagg tcctcgatcc cgagtcgcag
     3481 aaggtcgtcg acgcgatcgc caccacggct gtcgaccgta gtgaccggcc gaccgatccg
     3541 gtggtgatcg agtcgatcac agtctcctga cgagagcggg tcagggagag ccggcgtagc
     3601 cggcggcggt caatgcgtcg agcacgttca gcgggtcggt gcccagatcc catcgggtga
     3661 agaccagcag ccggtcatcg gtcgtgtcga tttcgagcag ccgtaccttg cgggccaggc
     3721 ggcggaattc ggtaatccgg atgatcttga tgtcgtcgcg ccgcaagagg tttgtgcgtg
     3781 accagccgcg ggtaataagc ccgtcgtcgc tgattgccag tttcggccgg gcacgccaag
     3841 agaggcttgc aaacacaagc agaccggtcg cggcaatccc catcaggatg cgcccgggcg
     3901 catctgtgat cagggtcaca gtcgcgatag ccaagataag tccgaatatg ccgcacgctg
     3961 cgattccgag cgcgggtggg ctccattcag tttgctgcat cggcaattaa caccctttta
     4021 ccgttgctaa tgaggttatc cacaagtgct atccccaatg gggatgaatc acatcagtgt
     4081 gatctaatga ccgcaaggtt gctaaacggg taacgcgcgg aagccaagat aaattcatac
     4141 caatggtcgg cggggctaac gccagcgcat cgtgagcaac aagccggtga tcatgaaagc
     4201 aaaggcgatg gcgtagttcc acggaccgag ctcggccaac cacgggatcg cgttggaagc
     4261 gagctggaac accaatagcc aggccagccc gatcagcatc aagctgcaga acaacaccac
     4321 gaaccacacg ctcgacggcc cggacttgac cttgaccggc gtccggctca ccgggttgat
     4381 ggtgaagtcg ttcttcttgc gaactttgga cttgggcatt ggtaccttcg cggccactct
     4441 gcgtcacacg gtgcgacgat gtaactacga gcctaacgta ggcataggcc agccaagaat
     4501 tcgtggaggc gacccaatgg agccgagccg ccgttccgcc tggcggtttg gtgtgccggt
     4561 ggtctgcctg ctggcggggc tgctgctggc agctactcac ggtgtctccg gcggcagcga
     4621 gatccggcgt agcgacgcgc cgcggctcgt cgacctggtg cgcgaggccc agcagtcggt
     4681 ggatcggctt acggctcagc gggactcgct ggtgtcgacg gtcgacaatc atcacggcgg
     4741 ctcgcccggc gcggatgcgg cgttggcggc gatcaccaac cgctcggaca agctggcggt
     4801 cgatgcggga ctggacccga tgcgggggcc gggcctcgtc gtcacgctca acgacgcaca
     4861 gcgcgacgcg gagggccgct tcccgcggga cgcctccccc gacgacctgg tggtgcatca
     4921 gcaggacatc caggccgtgc tcaacgcgtt gtggagcgcc ggcgccgaag gcgtccagat
     4981 gcaggatcag cggatcatcg cgacgtcggc accgcggtgc gtcgggaaca cgctgctgct
     5041 caacggccgc acctacagcc cgccgtacgt gatcaccgcg atcggagacg tagccgcgat
     5101 gcaggcggcc ttggcggcgg caccgttggt gatcctgtac aagcagtacg tcgtgcggtt
     5161 cgggctgggc tacaccgaag aaccgcggcc gcaggtggac ctcgtcggcc acaccgagcc
     5221 cgtgcggatg aaattcgcaa agccagccgg tcccctcgga tattgagtct ggcgttcact
     5281 ctgcgcgtag ggcgcagaag tgcgagtagg cgacaccctc accgcagagt gaacgccgga
     5341 cgacccagta gcctgggctg atgcaggtct tggtcgtcga caactacgac agcttcgtgt
     5401 tcaacctggt ccagtacctg ggccagctcg gcgtacacgc gcatgtctgg cgcaacgacg
     5461 acgaccgtct ggccaccgac gccgacatcg ccaaggccgc agagcagttc gacggcgtgc
     5521 tgctctcccc cggccccggc acgccggagc gggccggcgc atccatcccc ctcgtcaacg
     5581 cgtgcgccgc ggcgaagacc ccgctgcttg gcgtgtgcct cggacaccag gccatcggcg
     5641 tcgcgttcgg cggcaccgtc gaccgcgcac ctgagctgct gcacggcaag accagcaccg
     5701 tattccactc gaatgccggt gtgctgcaag gccttccgga cccgttcacc gccacccggt
     5761 accactcgct gacgatcctg cccgacaccg tgcccgccga gctcgaggtg atcgcgcgca
     5821 ccgagggcgg ggtgatcatg ggtgtgcgcc atgccgagct gccgatccac ggcgtgcagt
     5881 tccaccccga gtcgatcctc accgaaggcg ggcatcggat gctcgccaac tggctcggct
     5941 tctgcggtgc cgcgccggac gaaaccctcg tgcgtcagtt ggaggacgag gtggccaacg
     6001 ccgtgcaggc cgctacgacg cgaacgccag cgtgatcggg tcgctgaact tgaccggcgt
     6061 gcccgccgcc gggctctgag taaccaccgc gttggtccgc tgtccgctgt tctgcacgtc
     6121 gggcccccgg atcagcgggc cggtccagcc gtaagcgttg cgcaggttcg gctcgacgtc
     6181 cgtccagaat ccgccgcgca cgtcgggcat cacgaactgg ttgcccatag acacttggac
     6241 ctggatcacg ctgtccagcg ggacgatgtc gcccgccggg ggaagcgtgc ccatgaccgt
     6301 tccctttggc gacggactgt caacctcgac cacgacaggc tttgtaaagc cggacgcact
     6361 gagcacagac tgcgccgatt cgaccgtctg gttcttcacg tcggggaccg gcttgctctc
     6421 aggcccagag ccgaccacga tcgtgatctc gtttgtgatc gcggatgtct ggttggccgg
     6481 cgggatggtg gccagcacct tgtccttcat ctctggtgtg gacggtgacg gcgacgccct
     6541 gaacttgccg aagcccgcat tgctgagctt ctgcacgcag tccgcgtagc tcacaccctt
     6601 gcagtccacg acctcgcgct gctcggggcc cgttgacacg ttcagggtga tctcgtcgcc
     6661 tgcgccgaca gacgtgcctg cgctcgggtc ggtaccgatg accttattcg gggggatcgt
     6721 cgagtccgga ttccgctcgg tcgtcgtctt gaagccggca ttctgcaata tcgcgatcgc
     6781 gtcgttctcc agcttgccct tcacgtcagg cacctgaaca tcgcgggtac tgccgccgaa
     6841 catattgatc gcgacggtca ccacgacggt caaaaccgcc aacacggcca ccgcgatgag
     6901 ccaacgaccc accgagccgc tgcgctcgtc cttgtcgtcg tacgcagcct gcgggcggcc
     6961 gacctgatcg atgggctccg tccggtcaga agggggccgc gacgatgaca tcagcgaggt
     7021 gcgttcggcg tcggtgagca ccttcggcgc atccggcgtc tcgccgctgt gcacgcggat
     7081 cagatcggcg cgcatctccg ccgcggtctg gtaccggttg tcggggttct tggccagcgc
     7141 cttgagcacc acggcgtcga gttccggcga gatgtcgttg tgccgctgcg acggcggcgt
     7201 tggatcctca cgcacatgct gataggccac ggcgaccggc gagtctccga tgaaaggtgg
     7261 ctcgcccgta aggatttcgt acagcacgca acccagcgag tacacgtcgg agcgtgcgtc
     7321 gaccttctcg ccgcgggcct gctcgggcga caggtactgt gcggtgccga tcaccgcagc
     7381 ggtctgcgtg acgctgttgg catcggccag tgcgcgggcg atgccgaagt ccatcacttt
     7441 caccgcgccg ttcttgctga tcatgatgtt ggcgggtttg acgtcgcggt ggatgatgcc
     7501 gtgctggtgg ctgaagttca gggcctggca cgcgtcggcg atcacttcga tcgcccgctt
     7561 ggcaggcatc ggcccttcgg tgtgcacgat gtcgcgcagc gtcaccccgt cgacgtactc
     7621 catcacgatg tacggcagcg gcccggtcgc agtttccgcc tcgccggtgt cgtagaccgc
     7681 gacgatcgcc ggatggttca gcgcggcggc gttctgcgcc tcgcggcgga accgcagata
     7741 gaagctggga tcacgggcca ggtcggcgcg cagcaccttg atcgccacgt cgcggtgcag
     7801 ccgcagatcg cgcgccaaat gaacctcgga catgccacca aagccgagga tttcgccgag
     7861 ttcatatcgg tcggacaggt gctgcggggt tgtcatttcc atgtctgttc tggggcgatc
     7921 gcgtcgatct ctagtccacc atgacggaag gtgccctcat cggtccaacc gggcatcgcc
     7981 gacggcgact ggaacggggt cgtctcggtg atcgtattcg tcaccgtcgg tgaaggtgac
     8041 tgttggtctt tgcggtcctg cgcgttgagg acgatcaata tggcgatgac gatggccagc
     8101 gcgccgagca cgcccgccgc ccacagcagc gcgcgctgcc cggacgagaa cgtgcgccga
     8161 ggcgggggag tgcggtggct gcctgtcgca ggccgtgccc gggccgctgt cgcgggagcc
     8221 cggccagtga ggtcggcggc ggctcgtgcc tgggcggcgg atggcacggc cgctggtgtt
     8281 gcccggccga tggacggcgc ttgattcggc ctcggtggac gacggccgga ccgcaccgcg
     8341 gccaccgcgt cggcgaacgc gccgccggag cgataccgca tgcccggatt cttcaccagc
     8401 gtgatctcga tgagttcgcg aacgttgggc ggaagatcgg ccggcagcgg cggcggcgtc
     8461 tccttgatgt gcttcatcgc gaccgtcagt gcgccgtcgc cggtgaacgg ccgcttgccc
     8521 gaaaccgatt cgtagccaac aactcccagc gagtacacgt cgcttgccgc ggtggcgtcg
     8581 tggccgagcg cctgctcggg tgcgatgtac tgggcggtgc ccatcaccat gccggtctgg
     8641 gtgaccggcg cggcgtcgac ggccttggcg atgccgaagt cggtgagctt cacctgccct
     8701 gtcggcgtga tgagaatgtt gccgggtttg acgtcgcggt gcaccaagcc ggcggtgtgc
     8761 gcgacctgca gggcgcggcc tgtctgctcg agcatgtcga gcgcgtgccg taacgacagc
     8821 cggcccgtgc gcttcagcac ggagttcagc ggttcaccgt tgacaagttc catcaccagg
     8881 tacgcggtgc ggccctcacc gtccatctcg gtttcgccgt agtcgtacac gcctgcgatg
     8941 ccggggtggt tgagcatggc gaccgtacgg gcctccgccc ggaaccgttc gacgaactct
     9001 gcgtcggtgg agtactccgc cttgagcacc ttgatggcga cgcgacggcc caagcgggag
     9061 tcgatgccct cccacacctg gcccatgccg ccggtggcga tcagccgctg cagccggtag
     9121 cggccggaca gtgtcactcc cacgcggggg ctcattgctc gcctcccgct cgtcccctgc
     9181 cctgcgggcg tgggcccacc ggtcctcgcg cgtcccgcgc tgcgatcctc actcgcggtc
     9241 gccttcggga atcaatgcct cgactcccgc tcgtcccctg ccctgcgggc gtgggcccac
     9301 cggtcctcgc tcgttcctcg ctgcgatcct cactcgcgct cgtcttcggg aagctcatga
     9361 tccctcccgc agcgccgccg cgatcgtggc gcgcccgatc ggcgcggcca cggcaccgcc
     9421 ggtggcagac agccggtcac caccgttttc cacgacgaca gcgaccgcaa ccttgggggc
     9481 cgatgccggc gcgaaggcga tgtaccaggc gtgcggcggg gtattgcgcg gatcagtacc
     9541 gtgctccgcc gtgccggtct tcgatgcgat ctgcacgccg gcgatggctc ccttctgctg
     9601 cgtcacctgc tcggcggcga ccatcaaatc cgtaagtgta ttcgcgacct gctcggacac
     9661 cgcccggcgc tcctcgcgcg gagccgtcgt gctgatgttc gcgaggtcgg gtcccttcag
     9721 actatcgaca aggtacggct gcatggtcac ccctttattc gcgatggtcg cggcgaccag
     9781 cgcgttctgc agcggcgtca atgcaacatc tttctgcccg atgctcgaca tacccagggc
     9841 ggcggcgtcg gtgatcgggc cgacggtgga ctcggacacc tgaagcggaa tcgtcggcgg
     9901 agggctgtcc agaccgaacg ccagcgcggt ggaccgcagc gcgtcggcgc cggtgttgag
     9961 gccgagctgg acgaaggcgg tattgcagga tttcgcgaag gcgtcgcgca gagacgcggt
    10021 cggaccggcg ccgcacgacg caccgccata gttctccaac gttgcggtgc tgtccggcaa
    10081 cgtgactcgg gacgcagcgg tcagctgggt gttgacggtg gcgccggact gcagcgcggc
    10141 agccgtggtg atgaccttga aggtggaccc cggcggatag gtttccgaga tggcacggtt
    10201 cagcagcggc gagtcagggt tgtcgcgtag cctctgccac gcggccgact gtgcctgaat
    10261 gtcatgagtg gcaagcaggt tcgggtcgta cgacggggac gagaccatcg cgaggacctt
    10321 gccggtcgac ggctccagcg ccaccaccgc gcccttgcac ggaccgttgc agccgtcctc
    10381 catcgcatcc caggccgctt gttgcacctg cggattgatg gtggtcgcga cgttgccacc
    10441 gcggggatcg cggccggtga agaagtcggc cagccgccga ccgaacagcc gctgatcgga
    10501 gccgttgagg atggtgtcct cggcccgctc caggccggtg ctcgaatagc tcagcgagta
    10561 gaagccggtg acgggggcgt aggccagcgg gttggggtag acgcgaagga aacggaaccg
    10621 gccgtcggtc gataccgagt aggcgagcag ctgcccaccg gcggagatct gaccgcgttg
    10681 acgggagtac tcgtcgagca gcacccgctg gttacgcggg tcggcgcgca aaccatcggc
    10741 tgtaaacact tgtgtcactg tggcattggc caacagcagc acgatcagca ccatgattgt
    10801 cacggcgatg cggcgcagtg aggtgttcat accttctcga tcacctcggt acccgcggcc
    10861 gcgatcggtt gcgagtgcgg ccgggtgacg atcgggcggc gggcggcgtg cgagatgcgc
    10921 accagaatcg ccagcaggac atagttggcc agcagtgacg aaccgccgta cgacatccac
    10981 ggggtggtga gtccggtcag cgggatcagc ctggtgacac cgcctaccac gatgaacaac
    11041 tggatcgcca gcgtcgaagc caggccggcg gccagcagct tgccgaagct gtcccgcacc
    11101 gcgatcgcgg tccgcaggcc gcggatgatc acgattgtgt acagcatcag cactgcggcc
    11161 aagccgacca gcccgagctc ctcaccgatc gcggcgatga tgaagtcggt cgacgcggcg
    11221 ggcacggtgc cgggctgacc attgccgaga ccggtgccga agatgccgcc ggtggcgaag
    11281 ctgaacagcg actgcaccat ctgatacccg gcgccgtcgg gatcggcgaa cgggtcgagc
    11341 caggtttcga cacggacctt gacgtggtcg aaaacgtaat acgccgcaac gcttcccgcc
    11401 gcgaacagcg ccagcccgat gaccacccag ctgaaccggt cggtggccac atacaccagc
    11461 accaggaacg acgcgtacag cagcagcgag gtgccgaggt ccttttcgaa gaccatcacg
    11521 gcgacggagg cgatccaggc cgccagcagc ggagccagat cacgcggccg cggtaggtcc
    11581 atgccgagga agtgcttgcc ggcactggtg aacacgctgc gcttggccac caacaccgcg
    11641 gcgaagaaaa tgagcagcag gattttggag aactcggcgg gctgaattga gaagcccggc
    11701 agccggatcc agatcttcgc gccgttctgc tcggacatcg accgcggtag caccgcggga
    11761 atcacgagca ggaccaatcc cgtcagaccg cagacgtagc cgtagcgggc caacatgcgg
    11821 tggtcgcgca ggaagatcac caccagcgaa aaaccgatca cgccgaccag cgtccacaac
    11881 atctgttgat tggcggtgcc gcccaagcct ttcgacatcg gcgtgcccgc cgccaggtcc
    11941 aggcgatgaa tcatgaccag accgaggccg ttcaacagcg cgaccaccgg cagcagcagc
    12001 ggatcggcgt acggggtgaa ccgtctgatc gccaggtgcg caccggtgaa cagcgcgagg
    12061 taggcgacgg cgtactgggc cagatcccaa tgtaggccct gttcttggtt ggcctcgacg
    12121 agcagcagcg ccaccgtggt gagcagcgcg gcgaagccga gcagaaacag ttccgcgttg
    12181 cgacgattcg gcagcggcgg catgacggtg acaggcgact gcggctgggt ggtcatgcct
    12241 cgactcccgc tcgtcccctg cccttcgggc gtgggcccac cggtcctcgc ttcgcgcggg
    12301 agcttgcggt tcgcgccatc gacctgagcc tcgctgcgat ccttactcgc gctcgtcttc
    12361 gggtggtcat gacacttccc ggcagttcgt tcccggttct ggtggcgggg gaggcagcgc
    12421 ggtcacggtg ggctgcggcg agggggtcgg cgacggcggc ggcgcgctcg gcggcggcgc
    12481 ccctggcgga ggcggacccg gtggtggtgc gggcgccgac gcagtcacgg tcgtcggggt
    12541 ctccggcgcc ggggtatcac cgcccgtctc gggaatgccg ggattgaccg gcgtttgcgg
    12601 cagatgcggt gtcggcgatg tcgtggtcgt agtcggggtg cgcgtggcgc acaccgggag
    12661 caccgaacta cgggctagct cttcgatctg ggcgatggcg tcatcgagcg tgcccgaggg
    12721 aaggcccgca accacctgcg cgcgctccga cgggcgcatg tcatcgacgc cgagcatgcg
    12781 gcagcccgaa ttgttcttgg attgcccggc gctgatgagc gacaactcat ttcgcgcgtt
    12841 gagacagccc aacaggtagg gctcctgaag agctaaaccc agaaaggaac cctgaacacc
    12901 ccgcatgatc gacacggtgc cgtcgtgctc ggtgacgtaa tagttgctgc gcacaatttc
    12961 acggccgacg gccaagccgg ccagcacgac caggaccagc agcaccgccg cgatcagcat
    13021 gcgccgccgc gaccgcggtt tgcgctgcgg ctcttccggt tgcggcacaa cgcgtttggc
    13081 ctcgttgcgg cgtggattga acgccgacgc gcgacctgcc gcggtgtcgg gcggcgcgac
    13141 ctgatcgtcg tcaccggaca ccgcgccggc gaggatgggc tgagtctggc cgtagtcgta
    13201 gtcgatgacg tcggctacca ccacggtgac gttgtccggc ccgccaccgc gcaacgccaa
    13261 ttcgattagc cggtccgcac tttcggccac atcggaaatc tgcaacgcct cgaggatcgt
    13321 ctcgtggctg accggatccg acaaaccgtc ggaacacagc agataccggt caccggcccg
    13381 cgcctcccgc atgatcagcg tcggctccac ctcatgcccc gtcaaagccc gcatgatcag
    13441 cgaacgctgc gggtggctgt gcgcctcctc cgcggtgatc cggccttcgt cgacgagcgt
    13501 ctggacgaac gtgtcgtcct tcgtgatctg ggcgagttcg ccgtcgcgaa gcagatagcc
    13561 tcgcgagtcg ccgatgtgca cgagcccaag cctgttgccc gcgaacagaa ttgcggtcag
    13621 cgtcgtgccc atgccctcga gctccgggtc ggcttcgacg tgcgcggcga tcgccgagtt
    13681 gccctcgcga acggcggcgt cgagcttgga gagcagatcg ccccccggct cgtcgtcgtc
    13741 gaggtgggcc agcgcggcga tcaccagctg cgaggccacc tcacccgcgg cgtggccacc
    13801 catcccgtcg gccagcgcaa gcaggcgcgc gccggcgtac accgagtcct cgttgttggc
    13861 gcggaccaaa ccgcggtcgc tgcgggcggc gtatcgaagt accagtgtca cgggcgcaac
    13921 tcgattaccg ttttgccgat tcgaaccggc gtgcccatcg gaacctttac cgccgtcgtc
    13981 accttcgccc tgtcaaggta tgtgccgttg gtcgatccta ggtcctcgac ataccattcc
    14041 gaacctcgtg gcgaaagcct ggcgtgccgc gtcgaggcgt agtcgtcggt gagcaccagc
    14101 gtcgagtcgt cggcccggcc gatcagcacg ggctgcgtgc ccaacgtgat gcgggtaccg
    14161 gtgagcgcac cttcagtcac cacaagctga cgcgccacat ggcggcgccc acggttgggc
    14221 agcagcgagc cgcgcagcgc cagcccccgt cgcaccatca ccgcccccgt cggcgcgtag
    14281 atatcggtcc gcaggatccg gagcaccgac cagatgaaca accacagcag caacaggaaa
    14341 ccgacgcgcg tcagctgcag tactaacccc tgcatctgac gtcctctccg tccccgtccc
    14401 gttctcgtcg cgccggcaac gcaatcaact caagcttcgg caacgtcacg atacttggac
    14461 ggcgatcggc gtgcgggtga agcttcagct tcccccggcg gacgtcgacc agaaccgggc
    14521 ctagacctca gtgaacgcgg acgatgatct cggagtggcc gagccggatc acgtcgccgt
    14581 cggccaactg ccactcctgc accggcgcgt tgttcaccgt cgtgccgttg gtggaattga
    14641 ggtccgacaa cagcgcgacc tgaccgtccc agcggatctc cagatgccgg cgggacaccc
    14701 cggtgtcagg cagccggaac tgcgcgtcct ggccgcggcc gatcacgttg gcaccctcac
    14761 gcaactggta agtgcgcccg ctgccgtcgt cgagttgcag ggtgacgctg gtgccgccgg
    14821 ccgggtagcc gccctcggcg tagccgccgc cataaccctg cccggtgggc tgaccgtagt
    14881 cgtagccgcc gccggcgggc tcgccgtagc ccggttcggc gtacccggcg ccggccggtg
    14941 cctcgccgta gcggccgtag tccggctgcg cgtagtcctg gcggccgtac gcctgcccgc
    15001 cgtagccgcc ctgatccggg tagccgccct gatcggggta gccgccctgg tcggggtagg
    15061 ccggacgcgg ctcggcacgg ccgtagccct catcgggacg gccgggtgcg ggctggcgac
    15121 cgtagtcgta gtcgccggcg ggagcgccat agccctgctg tccgccttgc ggcggagggt
    15181 agccgccggg cgcctgccgg taaccctggt cctgatagcc gccaccggcc ggtgggccgt
    15241 agccggcggg cggacgctgt tcgtatgacg gcggcgggta gccctggtcg gggtagccgc
    15301 cctgatccgg gtagccagcc tggtcgggat aaccctggcg cggcgggtag ccctgctcag
    15361 cctgcggcgg gtagccgccg ccctgctgct cttgtggggg gtactgaccg cgcggatcct
    15421 ggccgcgctc atcttgcgga cggccgtagc ggtcgtcgta atactcgtcg gcgggccgac
    15481 cctgtccctg gccgccgccg cgatagctcg gattgtcgct catcggtggt actcctggtt
    15541 ctgcgttgga cgagagttct cgaggtgcgg cgggctcgcc ggtggtcgta tcaggattga
    15601 ccgcaccacg cgcccgaaac tgtccggtgt gcaggttcgg tgacgcctcg aatctgacga
    15661 ccacatcacc atacgtttgc cacccctgct catggatgta tccctccaag tgcttggcaa
    15721 aagtcgttga tgtgaggtcc gggtcggcgc tcaccttctg atagtcaggc acactgaggg
    15781 taatgacgta gtcgtttggc gccaaaatgc ggtcgccgag cacctcgcgg gcaccggtgt
    15841 cggcttcccg gcgaagcatc gcctcgactt cttgcggcac aatcgatccg ccgaaaaccc
    15901 gggcgaaggc gtcaccgacg gtggattcga gtttgcgctc gaagcggtct acgagaccca
    15961 tgtcaccgcc cgcctcactc gtcgttgtcc gtcggtcctg ctcaggcacc aacgcgccag
    16021 cgtgtcgcct ggccacttgg cttacctgca tggtatcggc ccagcgcagc gctgcgggac
    16081 caacagaatt ctgagaatcg aaacgggcca ggtcagctgc ctgatcgtga tcggctgaca
    16141 gcggattgac atggcgtgag ccccgatcgg tacggttgag gagcggggtg cctcgcgtga
    16201 taggctcccc cggttgttgg ggcgagtggc ggaatggcag acgcgctggc ttcaggtgcc
    16261 agtgtccttc gggacgtggg ggttcaagtc ccccttcgcc cacaaacaac gagcgtcaat
    16321 gcccttgtct cgcagtgtat ttcagcgagt tggcgggccg ccgcgcatat gggctagccg
    16381 gtcggtgcca ccgagaatcc cggcggggcc gacagctgca gaacgtcgcg caatcgacgt
    16441 ccgagcttgt cgagccggtc ggcgcccatc cgattgctcg gcccggtgac gctcaccgcg
    16501 acggggcgtc cggttgagcg ccgcagcgcc acacccacac agcagattcc ggcttcgttt
    16561 tcctcgcgtt cggtcgcgaa tccgtgcaac cgtgcctcgg caactgcccg ttcgaaccgc
    16621 ttggcaacct tggggtcggc attggcggtg tacgccgaca gttgggtggt ggctccttcg
    16681 gcggcgatca gcgcgcgccc gagcgcggtg gtcagcacgc tgacctggcg gcccacccgg
    16741 gaccacacgc ggattgtgcg gtcaggttcg accttgtcca gatagagaac ggtcgggcct
    16801 tcgaggatgc ccaggtgtac caattcttgg gactcctgac agatggccag cagcgcgggg
    16861 cggaaaaggg tcggcaggtt ttcctcgcga agaaactgct cgaccaagag cgccggacgt
    16921 gagcccagcc gatagcgctg attggcgtcc tgttcggcga agccgcggtg ttgcagggcc
    16981 tgcagggtgc gatgcgcgga cgccttgttc agtccgctcg cctcgaccac atcacgtaac
    17041 gccagcccgt ccgggccggc ctcggcgagc agttcgatga gggcgagcgc cttgtcgaca
    17101 ctcccgaccg ggggagcagc cttgctatcc acagtcgctg cctcccatag ccagagcttg
    17161 gcacacccgc ggtagtgagc cttgacacac tgcggccgac atgcgagtgt ggttcacact
    17221 acgatacatg cggttcataa tacggaacac ccaccgcaat cgctacggcc gacatactca
    17281 ggagcgcacc aatgcgacga ctgtcccgcc ggaccactcc ggatggtgcc atgatcggtg
    17341 cgtcagctgg ggctgcgacg ggcatcgtcc atctgggatt gggcaacttc caccgcgctc
    17401 atgccgcggt ctacaccgca aacgcgttgg ccgccgaacc cggcgactgg ggcatctacg
    17461 gattcgccaa tcggtcccac gacgtggtct cggcgatgcg tgcccaggac gggcgatacg
    17521 cgatcttgga actgtccgac cgcggtcgcc aggtcggcat tgtagatgtg catcgcggtc
    17581 tgggcgttct cgccgacgac cgcaccgcgt tcgtggcggc cgtcgcggac ccgtcacagc
    17641 gaattctgac gctgaccgtc agtgaggtcg gctactgccg ttcgccccgc accggcacgc
    17701 tcgacgtcga ccgcgacgat gtacgcgccg atatcgccga ccccgctcat ccccgcagca
    17761 ccatcggact cattgcgcgc ggcctgtcgg tccggtcggc gagcggggct ccgataacgg
    17821 tgctgtcgtg cgacaaccta cagtccaacg ggcactccac ccgcgcagcg ctcaccgagt
    17881 ttctgcgcgc cagtgcggcc tctgacgacg cgctgaactt ccttgaaaat cacgtcagct
    17941 tccccaattc gatggtggat cgcatcgtgc cggcgaccac tgcccagacg aacgcagacg
    18001 tcgcagagct gatcggcgta gccgaccacg ccccggtgcc caccgaagac ttcagcatgt
    18061 gggtcctgga ggacgacttc gccgccgggc ggccggcgtg ggatcaggcg ggggcgatca
    18121 tgagcgacga ggtggaggcc tacgagctgg tcaagctgcg gctgctcaac gggtcacatt
    18181 cgctgatcgc gtacctgggc gtcttggatg gtcgggccac catcgccgac gcctgggccc
    18241 aggatttcgt ccaggacgcc gtccggacct gtatcgcacg cgagtacttg ccgactatcg
    18301 cactaccgga ggggttcgat cccgactcct acctcgagtc tctgtcgcac cgctgggcca
    18361 acgccgcact gggtcatcgg acgtcacaag tgggcaccga cggttcggtc aagctcctgc
    18421 agcgcattcc tgagcccgcc cagatcgcgt tgcgcgccgg ccgggttcca catctgttgg
    18481 cgctgaccgt tgcgggatgg atctgtgcag cggccccacc gacgggcttc gatcccggcc
    18541 cgaacgccgc tgagatccgc gaacccgcgc ggcagcggct tgccgaggtg acccaaggtg
    18601 cgggcggacc gcgagagcac gcaatggcgg tgctgcgcaa cgggtttctt cccgattccc
    18661 tcaccgcgca tgcggcattc acagaccgag tcgcggattt ggttgaggta atagcgcgca
    18721 acggagtgcg tgcggccgcg cgcgaggcca tcgaggttga caacgacagg aaagtgtcat
    18781 gaaagcgctt gcgattcacg gcaaagagga catccgctgg gaggactgcg ccgcgccggt
    18841 accggccgac ggacaggtgc ggctgcgcgt cagctacgtg ggcatctgcg gttcagacct
    18901 gcactactac ttttacggag ccaacggcga gaacgttgtt cgcgagccgt tcacgcccgg
    18961 ccatgaactg tcggcggtgg tggatctcga cccgtcgggg cgccttccgt ccggcgctcc
    19021 ggtgaccgtg cacccggccc gctacgggca acccgtgccg ggtctggctg accgtccgca
    19081 tctgtgccct ggcggtgact atctcggcag tgccgccact ttcccgcatc gccagggcgg
    19141 cgcggccgaa ctgctgatcg tcgaggacca catggtccga aagctgcccg agggattgcc
    19201 gctgcggcgc gcggcgctgg ccgagccgct ggcggtggca ttgcacgcgg tcgggctggc
    19261 aggcgatctc accggtaggc gtgtgttggt gatcggtgcc ggccccatcg gcctactcgt
    19321 cgtagccgct gccctcgatc gcggcgcgac cgagatcggg gtgagcgact tgcggccgga
    19381 accgttggcc cgagccgcag cgctgggtgc aaccgaaccg gtcctggtcg gccgcgacag
    19441 catcgccgac gagtcattcg acgtcgtttt cgagtgctcc ggcgtcgggg tgtcgttgac
    19501 acaggcggtg cgtgccgccc gacgcgccgg gacggtcgtg caagtgggca ccctgccgaa
    19561 taccgagatt tccgtcaacc tggcgccgat gctcggtaag gaactacgcc tgctgggggc
    19621 gtttcgcttc gccacggaaa tcgacgacgc gatcgcgatg ctcgccgcct ctgatcacct
    19681 cgaatcggtg atctcccatg tcatcccggc cgctgaggcc gtagacgcct tcaccctggc
    19741 ccgcgacgcc tctgcctcgg ccaaagtact gctccaactt tgacattcag atctgcaaac
    19801 caacgaatgc aacccaacga agaggaagaa cgatgactac cacccccgaa cctcggaccg
    19861 ccgagctgcc gtccaccgcc cagcgcagca tggccgacct ggtgcgcgcc gccgtgtccg
    19921 gatggctcgg caccgcactg gaattcatgg acttccagtt gtattcactg gccgccgcgc
    19981 tggtgttcac tcaactgttc ttctccggtg agagtgcggg gatggcggtt gtgtgggcca
    20041 tggcgaccta cggggtcggc tacatcgccc gaccgctcgg cgccatcttc ttcggacgcc
    20101 tcggcgaccg gatcggccgc cgcaaggtgc tcttctacac catcgcgctg atgggtgcgg
    20161 caaccacgct gatcggtgcg ctgccgacct acgcccaggt gggcatgctg gcgccgatcc
    20221 tgctggtgag cctgcgactg ctgcagggat tcggcgccgg cgccgagatc agcggtgcgg
    20281 gagtgatgct cgccgaatat gcccccgcgc agcggcgcgg gtttatcgcc tcgctggtgg
    20341 gcctcggcac caactgcggc accctgttcg cgtcggcgat ctgggccctc ctgctgggcg
    20401 tgatggtcga aagcgacgtc atcgcgtggg gctggcgcat cccgtttatc gccagtgccg
    20461 tggtgatggt cttcgcggtg tgggtccggt tgaacctcaa ggaaagcccg gtgttcgagg
    20521 agcgtgacga cgtcgtcgac gggcgcgcac tgtcccgcga gcagatgatc gaacaggccg
    20581 aggagcacag cgacacccgc accctcgaag cggtggagcg taagccgatc aaagcggtcc
    20641 tggtgtcgtt cttcctacga ttcgggcagg ccggcaactc cggcctcatc cagacctacc
    20701 tgatctcgtt catgaccatc acgctcgcga tgcagaagag cgtcggcgcc aacgccgtca
    20761 tcgtgtcctc actggtcggg ttcctcacca tcccgctggt cgggtggctc ggtgaccggc
    20821 tcggccggcg ccggatgtac atcgtcgtga tgtcggcggc gctggtcgtg atcgttccga
    20881 caatgctggc gatcgactcc aagaacctcg tgtgggtgtt cgtcggctac atcgtgctgc
    20941 acaacgtcgc ggtgctgtcc ccggcctcgc tggagaacgt ctcgatcccg gagatcttcg
    21001 gcgcgcgcaa ccgctacacc ctcaccgcaa tcatccgtga gatcgccgcg gtgatcgcca
    21061 ccggcatcgg ccccgtcgtc gccgccgcat gggtcgccgc ggccaccggc agccccatcc
    21121 cgatcatggt gatgctgggt ctgtactcag tggcggcgct gatcgcggcg gtgcgggcga
    21181 aggactggac aggacgcgat ctgaccgatc cgcgtccggc gatgtgagcg cgcatgacca
    21241 tcgagaagat cgacgtcaac gtctgcagtc cggggcgcaa cttcgtcacc ctcaagatcg
    21301 tcaccgctga cggcgtcatc gggtgggggg atgccacact caacggccgc gagctgtcgg
    21361 ttgccgccta tctgtcggag caccttgcgt ccacgctgat cggtcgcgac gaagaccgca
    21421 tcgaggacgc ctggcagtac ctctaccgcg gcgcctactg gcggcgcgga ccggtgacga
    21481 tggccgccat cgcggccgtc gacatggcgc tgtgggacat caaggccaag aaggccggca
    21541 tgccgctcta ccagctactc ggcggcgcca gccgagaccg cgtcagggtg tacggccacg
    21601 cctctggcgg cgactacgcc gcactaaagg acgcgatcag tggctacgtc gacgccggtt
    21661 acaccgccgt gcgggtgcag accggtgtgc ccgggcttgg acagatctac ggggtagcca
    21721 ccaacaagcc gggcacccgc tatgactacg aaccagccca ccgcgccgcc gatgggagct
    21781 ccgcgaccgc gccgatcgag gagacctggg acacccgggc ctatctcacc cacatgccgg
    21841 gggtgttcgc caagatccgg gaagacttcg gcaccgggct acgtatcctg cacgacggac
    21901 accaccggat gaccccaatc gaagccgcca ggttcgccaa aagcgttgag ccgtatgacc
    21961 ttttctggtt ggaggactgc acgcccggcg aagaccaggc agcactgagg ttggtgcgcc
    22021 agcattcgac cacaccgctg gccatcggcg aggtgttcaa ctcggtgtac gactaccaga
    22081 cactgatcac cgagcggctc atcgactacg tgcgctccgc ggtgactcac accggcggcg
    22141 tgaccgctat gaaaaagctg ctggacttcg cgggcatcta cggcatcaag tccggcatgc
    22201 acggacccac cgacatctcg cccgtcggca tgtccgcggc actgcacctc gacctggcga
    22261 tccacaactt cggtatccag gagtacatgc cgcacaacga cacgacactg gacgtgttca
    22321 aaacgtcgta cacgttcgac cggggttttc tgcatccggg ggaggcgccc gggctcggtg
    22381 tcgaactcga cgaacaggcg gcggcagcat tcgggtacac ggcggcgtac ctcccggtga
    22441 accggctgca ggacgggaca attcatgact ggtgacaccc tggcgcgcac gcagctgcca
    22501 gaccgcaccg cgacggccaa actgatcgtg gtcgtgcggg cagccgacgc tgccgactac
    22561 gaccgggtgc tcgatgctct gatcgacgcc gggatccgca gtatcgagct gaccctcacg
    22621 acaccgggca ccatcgaccg gctgccaaag ctgttaacac agttcggcga tactgccgat
    22681 atcggaatcg gcaccgtgac cgacgttccg cagctgaccg ccgccgtcga cgccggggcg
    22741 cactacctcg ttacaccgat cagcgacccc gaacttgtcg acgctgccaa ccgagccggg
    22801 gtgccaatcg tgcccggtgg gctgacgccg acggagctct tcgcggcatg gcgcgccggc
    22861 gcggccgcgg tgaagatctt cccggccaac caggtcggcc cgggatacct caacgatctg
    22921 cgcgggccgt ttcccggcat cgcggcggtg ccatcaggcg gtgtggacct cgagggcgcc
    22981 gcggcgtggc tgcgtgccgg tgcggcagca gtcagcgtgg gtggcccgct gctcggtgat
    23041 gccttgcgtg gcggcgactt tggcgcgttg cgcgaccgga cgacggaatt cgtggcgacc
    23101 tgcgggcagg tgacgacgtg accgccaggg tcgccaccgt cggcgaaacc atggccctga
    23161 tgcggacgac ggacatcggc tccctgcggc acgtgtcgga tatggtcctc ggcatcggcg
    23221 gtgccgagag caatgtggca atcgggttgc gccgcttggg agtcgatgtc acatggttgg
    23281 gtcgggtcgg cgacgacccg ctgggggagc gagtcactcg cgagatccgg gccgagggcg
    23341 tggacgttcg cgctgtgatc gacacgcagg caccgaccgg cttgatgctc aaggagcgac
    23401 catctgccag ttcgaccgcg gtgttctact accgcgccgg ttccgctggc tcgcgcctgc
    23461 agcccgacga tatgccgcca ggctggatcg agaatgtcga gctggtgcac gtatccggta
    23521 tcaccgcgtt gttgtccgac agtgcgcggg actgtgtgct gacggcgatc agccgcgcac
    23581 aagcctccgg ggttcaggtg agcttcgacg tgaactatcg gtcaacgctg gctcctgccg
    23641 atgtcgccgg accgctgctg cgcaagatcg ccgagaacgc cgacttcgtc ttcggtggtc
    23701 ccgaggagct gacgctgctc taccccgaca gcgagccggc cgaggccgct cagcgcctaa
    23761 tgcgcagcgg ccgtcgagaa gtggtgctga agaagggtgc cgacggagcg gccgtctatt
    23821 cgtccgacgc tgttgtcgag agcgctggat tcaccgtcga cgtggtcgac accgtcggag
    23881 cgggagacgc cttcgtcgcc gggtacctca gcgggctgct gcacggcctc gacgttccgg
    23941 gacgcctgaa tcgtgcaaac gcgtgcggtg ccatgctgtg catgacccct ggtgactggg
    24001 agtccagccc gacattgagc gatgtcgagc gattctgccg gccgggaacg gatccagtcc
    24061 tgcggtagct tttgcgaatg cggctcgcgt atatcgcgtt tgtttcagtg gtgctgctgc
    24121 tgtcggcggc gtgttccaca gcggtccaag gttcggcggt caaggagtcg ggtgcgcggt
    24181 cgggccccac gatcgacgtc agcaggctcg atgtcgggcc atacccgacg gagccgagtc
    24241 agccgctggg tgtgacgggc gacccggagc gcggagtcct ggtcgaggcc cagcgcatgg
    24301 ccaacaacgt cgtcgggccc tgggaagtcg actcgtcgct gaccggctgg ttcagctttg
    24361 gggccacggt gctgccgagc ccagacgcgc tggcgcagat cggcccggag ccgtttgcgg
    24421 cggccgcggc ccagcacggc ttcatcaacg gcttcgcgtc ggcgcggacg gcggagggtc
    24481 agaagattct gctcaatgcg gtcttgcggt tcgacgacac ggccgccgca gccgcggccg
    24541 ccacagagtt cggcgacatc gcggcaagga cgggtgaggg agtgcagcgc gcccaaattc
    24601 cgggccatcc ggatacacag gcggcgagct acacgcagac cgaggactcg accgggaagc
    24661 ggtggagcgc ggttcgggcg ttcacgccgc acggccagta cgtgttcatg caactcgcgc
    24721 aggccgtcga cgggatggac gcggccatcg gtttggtcgc cagcaccgtt gacgcacaag
    24781 ggccgatcat cgacaagttc cgcgcgaccg atccctccga gttcgccgac atctcgctgg
    24841 acccgaccgg cctgttggcg cggacccttc cggttccgga gaaggaagcg accaccatcc
    24901 aggacgccac ctatgaacaa cgcggcgcgc tgcacttcca aagcgatccg gcccgctcgg
    24961 cgaagctgtt caccgacacc gaaatggatc tggtggcgat ggcgagaacg aatgtctatg
    25021 agaccaagga ctccgcgagc gcggtgaaga tcgtggacgg gttcttcgcc gaattgcagc
    25081 cgacgtcgca gccggccaaa ccggtcaaca atctgccgga cagccgctgc ctgcagctga
    25141 aggacaagag cttctactgc cttggcgcgg cggaccgcta tgcgatcgaa accacctcgg
    25201 acaagctgct cgatgcccag caacaggtgg cggcgcagta cgcgatgctg ctgagcggct
    25261 agcctccggc gaggacgccg cgcagaacca gctcgctcag ttccttggcc gcggtgtcga
    25321 cgtccttgat gcggcgcacg cacaccgcgt accaggcgcc gcccgcgatc gcgtccatga
    25381 gggtgtcggc gctgatgccg ggacgcgcgg tgccgtcggc gacggcctcg cccacccgct
    25441 gggcgagctg actacgcgca gtggcttcca ggcgatcgct gagcacccgg cgcatcgctc
    25501 ggtccgaccg taaatcgttg agcaggccgg gaattgattc gcgcactgcg gcgtcgccgt
    25561 acatccgcgt cgcaccgcgg cacagccggg tgatctccgc ggcaaagtcg tcctccggtg
    25621 gctcgggtcc gaggtcgggg aagagggcct cgtgcaccag ctgcgccttg gaactccaac
    25681 ggcggtacac ggcgggccgg ctgaccccgg cggtggtggc gatcaggtcg atcgtcgtcg
    25741 ccgcgtaacc acggtcgacc agcagccgcc gcgccgccgc cagcaccgcc ttgtcgatcg
    25801 agggatcccg gcgcgaaccc tgccgccgat gccgtcgctc cattcgcttc ccaccgtctg
    25861 agtttgtcgt tacagtgtgt cacaacaaag ccgatcggag ggcgctatga cgactgggct
    25921 caccaatccg ttggcaggcg tcggcgaaga cggtcggcac ctctacacct gtccgctctg
    25981 tgaggccatg tgcggcttgg agattcacgt cgccgacggc cgtgtctcga ctatccgcgg
    26041 caaccgcgac gacacctgga gccacggtca catctgcccc aagggcgcca ccctcggcgc
    26101 ggtgcacgaa gaccccgacc gcatccggcg cccgttgatc aaggtcgacg gccaatggca
    26161 ggaggtcagc tgggacgccg ccttccgccg ctgcaccgag ctgttggccc cagtgatcga
    26221 gaagcacggc atcggcgcgg tcacgtgcta caccggcaac ccgctcgcgc actcgttctc
    26281 cctcggccgc tacaccggcg tgctgctggg catgtcgggc atcccgctca gctattcgcc
    26341 cggcaccgtc gaccagtggc cgaagaatct gtcgtcgcac ctgatgtacg gcggctggtg
    26401 gacgttcccg gtacccgaca tcgagcgcac cgacctgctg atcgtcatgg gcgcaaaccc
    26461 cgcggcatcg caggggtcat tgctggccgc gcccgacgtg atgggcatca tcgacggaat
    26521 tcgtaagcgg ggcaaggtga ttgtgatcga tccggtgcgt accgccaccg ccgcgcgagc
    26581 cagcgagtgg ctgcccatcg caccgggcac cgacgcggcg ctgctactcg ccgtcgtgca
    26641 caccctgttc gacgagaacc tagtcaagct cagcgacctc gaaccgcacc tcgacggcgt
    26701 ggaccgactg cgcgaagtgg ccgccggatg ggcgccggaa cgtgtcgcgg cggtcaccgg
    26761 catcaccgcc gaccgcatcc gcaccctggc ccgcgagctc gccactaccg aacgcgccgt
    26821 tgtgtacggc cgaattggct tgtgcaatca ggagtttggc agcctggcca gctggctggt
    26881 cgatgtggtc aacattctca ccgggcattt cgactcgcct ggcggatcga tgttcccgcg
    26941 cgccgcggcg tggtctgtca ccgtacagcc gatccccggc ctcgaggatg gtgcaccgga
    27001 gttcggccgg taccgcacgc gggtgcgtgg cgcgaaggaa gtcctcgggc aggtgccggt
    27061 gtcgtgcctg gcggaggaga tcgcgacgcc gggggagggg cagatcaggg cgttgatcac
    27121 ggtcgcgggt aatccggtgc tgtcgacgcc ggcgggtcac aagctcgacg aggcgctgcc
    27181 gacgctggac gcgatgatcg ccgtcgacct gtggctcaac gagaccaccc ggcacgccga
    27241 cgtgatcctg cccgcgccgt cgccgctgga gcagccacac cacgacgacc tgatcctcaa
    27301 cttcgcgatc aacagcatcg cgaattactc gccgccggtg ttcgcgccgg aggacccgga
    27361 ccgaccggag gagtgggaga tcctgatccg gctgaccggg ctgtgcaccg gcatgcccgc
    27421 cgaggacgtc gacgtcgcgg cgatcgacga cgggttcttc gactacatgg cgttcacgca
    27481 gggcctcgaa ggcgctgaaa ttcgcaagca ctacgagcgc ggcgggccgg agcggatcct
    27541 cgatttcacc ctgcgcaccg ggccgttcgg tgaccgctac ggcgagaacc ccgatggtgt
    27601 cacgctggag aagttgaagg ccgagccgaa cggcatcaac ttcgggccga tggtgccgca
    27661 ggtgcccgaa attctcggca cggccgacgg caagatccgg ctggcgccgc agtacctgct
    27721 cgacgacctg cctcggctgg ccgcgcgcct cgaccgtgtc ccggatgagc tcgtcctggt
    27781 cagtcgacgc catctgcggt cgaacaactc gtggctgcat aacgtcggac cgctgatgaa
    27841 gggcagggat cgctgcacgc tgctgatgca cgacgacgac gcgacgaagc gcggcgtggc
    27901 cacgggcgac ctcgtcacgg ttacctcgtc gggcgggcag atcgaagtgc ccgttgaggt
    27961 gaccgacgcg atcatgccgg gggtggtgtc gatgccgcac ggctggggcc acggcaagcc
    28021 cggcacccgg atgtcgatcg ccaacggctc gcccggggtg aacaccaata tcctgtcgcc
    28081 gccgacgttc ctcgacgaac cgtcgggaaa cggcgctctg aacggcatcc cggtgacagt
    28141 caccgccgcc gcgaactgat aggcataagg cgtgggcaca aaccaacgtg cgaagatcgt
    28201 catgaccgat gacgagatcg ccgagttcat cgagcgtagc cggaccgcaa ccatggcgac
    28261 cgtgcttccg gacggcaggc cgcacctggt ggccatgtgg tacgcggtcg tcgacggcga
    28321 gatctggttc gaaaccaagg ccaagtcgca gaaggcggtc aacctgttcc gcgacccgac
    28381 gatcacggtg atgatcgagg atgggcagag ctacgacacg ctgcgaggtg tgtcgatcga
    28441 cggcaaggcc gagatcgttg acgacccgga aaccattctg cgcgttggta ttagcgtgtg
    28501 ggaacgctac accgggccgt acaccgacga gatgcgcccg ttcatcgacc agatgatgaa
    28561 caaccgcatc tgcgtgcggg tcgtgcccag ccggctgcgc agctgggatc accgcaagct
    28621 cggcatggcc ccgatgccga tcggcggcag tacggcgcag tatctctgat gccgcaggaa
    28681 cgccccaagc agctggactc gccgctgctg ccgaagatct tcaagtacgc aggcaaggcg
    28741 catgtgtggg tgtatcgccg gaccggcgga aaggtcgggg gcaagtggcg catcggcgcg
    28801 ggcttccgca aaccggtgcc gacgctgctg ctcgagcatc gcggccgcaa gtccggcaag
    28861 gtcttcgtca cgccgctcct ctacctgctc gacggcgccg acgtggtgat cgtcgcgtcg
    28921 cagggcggac gggccgagga cccgcagtgg taccggaatc tggtggccaa cccggatacg
    28981 ttcgtcgaga tcggcgccga ccggcgcgcg gtgcaggctg tcacggccgg cccggaagag
    29041 cgggcgcggt tgtggcccaa gctggttgag ctctacgcgg atttcgacaa ttaccagagc
    29101 tggaccgacc gcgagattcc agtggtggtg ctgaagccgc gttaggggtg tggcgggctg
    29161 aagccggggt tcgacaaagc gttggccacc ccgctgccga tcgggccgtg ttgatcgacg
    29221 atcgtcgcgg tgccggtcgc taccccgtcg tggctgtagt gggtcagtgc tgccaacccg
    29281 agatacggtc cctgcggtag gcgggacagc gtcaacgtgt agtcggcgtt gatgaactgc
    29341 aaaccctctg caccgaagtg ggttagcgaa ctgaccatgt cgccggccat cgccgctctg
    29401 gtgaatggcg tgagctcttc gccgtcgacc aagggcctga tgtcgcgcac ccagatgtat
    29461 ttcggtcccg cgtgtgccca ggcatcgagc ccgaacccgg gccctggggt gtgatcgctg
    29521 cggccgtagg tccacaccag catcgccagg cctcgcggga tcggatcggg ggtgggcgga
    29581 atgggcggca tggacagcgg cgaggaccaa atctcgtcgg ctggctgctc gccccggcgc
    29641 agaaacagtc cgctcgcgcg ggcgacgacg gtgtcgtctt gcgtcatcac cgcgtcgacc
    29701 aaccgaagtc gtcggccctg acgagtgatc gtggtgtcga cgcgcaccgg tgcgagcgcg
    29761 gcgggccgga acaggtcgac ggtgagccgt gtgggctgga agtctggatc gccggcgtcg
    29821 cgttcgagga cgtggccgag gatgccgccg atgtagttgc cgctgat
//
LOCUS       DASRJA010000469         3842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_883870,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000469 DASRJA010000000
VERSION     DASRJA010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883870"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFC01_20745"
     CDS             <1..329
                     /locus_tag="VFC01_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012687766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN82088.1"
                     /translation="AENHHLIDAARLSLMRPGSVLVNCSRGALIDLDAAEEALHNGRL
                     GGLGLDVYDPEPPEHHPVFDHPNTVLTPHLMGFTQRAMALTIQASVLGATAVLRGEQP
                     IAVATP"
     gene            433..3840
                     /locus_tag="VFC01_20750"
     CDS             433..3840
                     /locus_tag="VFC01_20750"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013416593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HZN82089.1"
                     /translation="MFSKVLVANRGEIAIRAFRAAYELGAGTVALYPYEDRNSVHRLK
                     ADESYQIGSPGHPVRAYLSVEEIVSAARNAGADAIYPGYGFLSENPDLAAACESAGIT
                     FVGPSADVLALAGNKARAIEAAQAAGLPVLASSPPSSDVTTLLAAGEEMQFPVFVKAV
                     AGGGGRGMRRVSERSQLREAIEAAAREAESAFGDPTVFLEQAVIDPRHIEVQILADQA
                     GNVIHLFERDCSVQRRHQKVVELAPAPNLDPVLRERICADAVAFARQIGYSCAGTVEF
                     LVDERGNHVFIEMNPRIQVEHTVTEEVTDIDLVQSQLRIAAGETLADLGLTQDSITLR
                     GAALQCRITTEDPANEFRPDTGRITAYRTPGGAGVRLDGGTALGAEVGAHFDSLLVKL
                     TCRGRDYAAAVARARRAVAEFRIRGVATNIPFLQAVLDDPDFRAGTLTTSFIEQRPHL
                     LTVRSSADRGTKILKYLADVTVNKPHGQRPSTIYPADKLPPTDLDESPPPGSRQRLLS
                     LGPAAFAYTLRAQPALAVTDTTFRDAHQSLLATRVRTTGLLAVAPHVARLTPQLLSVE
                     CWGGATYDVALRFLHEDPWERLAALRTAIPNICLQMLLRGRNTVGYTPYPEQVTRAFV
                     QEATDTGIDIFRIFDALNNVDQMRPAIDAVRETGTALAEVALSYTGDLSDSGEDIYTL
                     DYYLRLAEQIVDAGAHVLAIKDMAGLLRPAAARTLVSALRANFDLPVHVHTHDTPGGQ
                     LATYLAAWIAGADAVDGASAAMAGGTSQPALSSIVAAAAHTDRDSGLSLDAVCDLEPY
                     WEALRKVYAPFESGLPAPTGRVYHHEIPGGQLSNLRQQAIALGLGDRFEEIEANYAAA
                     DRLLGRLVKVTPSSKVVGDLALALVGTGVDVDAFAEDPDQYDIPDSVVGFLRGELGTP
                     AAWWPEPFRSKALRGRGEAKPETPLSAVDETGLAGNSADRRATLNRLLFPGPTKEFLA
                     HRQTYGDTSGLSANQFFYGLRRGEEHRVHLEKGVTLLIGLEAVSDPDERGMRTVMCIL
                     NGQLRPVSVRDRSIASDIPAAEKADKTNRGHIAAPFAGVVTLTVTEGDKVNAGEPIAT
                     IEAMKMEAAITAPRGGLISRVAIAPVQQVEGGDLLAAVTVAEETGPE"
BASE COUNT          651 a         1317 c         1264 g          610 t
ORIGIN      
        1 ttgccgagaa ccatcacctg atcgacgccg cgcgcctctc gctgatgcgg cccggcagcg
       61 tactggtcaa ctgcagccga ggcgcgttga tcgacctcga cgccgccgag gaagccctgc
      121 acaacgggcg gctgggcggg ctggggctcg acgtctacga ccctgagccg cccgagcatc
      181 acccggtgtt cgatcatccc aacacggtgc tgacacccca cttgatgggc ttcacccagc
      241 gcgcgatggc actcaccatc caggcttcag tgttgggtgc tacggcggtg ctgcgcggcg
      301 agcaacccat cgcggtggcc acaccatgac catcagttgg cgtgatcggt gtcggcgtga
      361 tgggccgccg gtgctgtgcg agaaaccgca tgctgcccgt tcctgctcgt ccccaaagaa
      421 gggttttccc ccgtgttctc caaggtcttg gtcgcgaatc gtggtgagat tgcgatccgt
      481 gcgtttcgcg cggcatacga gctgggcgcg ggaacggtag cgctctatcc ctacgaggac
      541 cgcaactccg tacatcggtt gaaggccgac gaaagctacc agatcggctc gccgggccat
      601 ccggtgcggg cgtacctgtc cgtcgaggag atcgtgtcgg cggctcgcaa tgccggcgcc
      661 gacgcgatct atcccgggta cgggttcctt tcggagaatc cggatctcgc cgcagcctgc
      721 gaatcggcgg gcatcacctt cgtcggcccc tcggccgacg tgctggcact ggccggcaat
      781 aaggctcgcg cgatcgaggc ggcacaggcg gcgggattac cggtactggc gtcctcgccg
      841 cccagttcgg atgtgacgac cctgctcgcg gcgggtgaag agatgcagtt tcccgtgttc
      901 gtcaaagctg tcgcgggcgg cggcggccgc ggaatgcgcc gggtgagtga acgcagtcaa
      961 ctgcgcgaag cgatcgaagc cgctgcgcga gaggccgaat cggcgttcgg ggatccgacc
     1021 gtgtttctcg agcaggcggt gatcgacccg cgccacatcg aggtgcagat cctcgccgat
     1081 caggcgggaa acgtcatcca cctgttcgaa cgggactgtt cggtgcagcg gcgtcatcaa
     1141 aaagtcgtcg agctggcgcc ggcgccgaac ctggatccgg tgctgcgcga acggatatgc
     1201 gcagacgcgg tcgcgttcgc ccgccagatc ggctacagct gcgcaggaac ggtggagttc
     1261 ctggtcgacg agcgcggcaa ccatgtcttc atcgagatga acccgcgaat tcaggtggag
     1321 cacacggtaa ccgaagaagt caccgacatc gatctggtgc agtcccagct gcgcatcgcg
     1381 gccggcgaaa ccctggccga tctcgggttg acccaggact cgatcacgct gcgcggtgcg
     1441 gcgctgcagt gccgcatcac caccgaggac ccggccaacg agttccgccc cgacaccgga
     1501 cgcatcaccg cataccggac gccgggtggg gccggagttc ggctggacgg cggcaccgcg
     1561 ctgggcgcag aggtcggcgc ccacttcgat tcactgctgg tgaagctgac ctgccggggc
     1621 cgcgattacg ccgcggcggt ggcgcgggca cgtcgcgcgg tcgccgaatt ccgaatccgg
     1681 ggcgtcgcca ccaacatccc ctttttacag gcggttcttg atgaccccga cttccgggcg
     1741 ggcacattga ccacatcgtt catcgagcag cgtccgcacc tgctcacggt gcgctcctct
     1801 gccgaccggg gcaccaagat cctcaaatac ctggccgacg tgacggtgaa caaaccccac
     1861 gggcagcggc cctcgacgat ctatccggcc gacaaactgc ccccgaccga cctcgacgaa
     1921 tcgccaccgc ccgggtcgcg gcagcggctg ctttccctgg ggcccgcggc gttcgcgtac
     1981 acgctccgcg cgcagcccgc gttggcggtg accgacacga cgttccgcga tgcccatcag
     2041 tcgctgctgg ccacgcgggt gcgtaccacc ggactgctgg cggtggcgcc gcacgttgcc
     2101 cgcctgacac cgcaactttt gtcagtcgag tgctggggcg gggcaaccta cgacgtcgcc
     2161 ctgcggttct tacacgagga tccctgggag cgactcgcgg cgctgcgcac ggcaatcccc
     2221 aatatctgcc tgcaaatgct gctgcgcgga cgcaacaccg tcggctacac gccctatccg
     2281 gagcaggtga cccgggcgtt cgtgcaggaa gccaccgata ccggtatcga catcttccga
     2341 atcttcgacg cgctcaacaa cgttgaccag atgcggcctg cgatcgacgc ggtacgtgag
     2401 acggggaccg cgctggccga ggttgccctc agctacaccg gtgacctgtc cgactcgggc
     2461 gaggacatct acaccctcga ctactacctg cgactggcgg agcagatcgt cgatgcgggc
     2521 gcacacgtgc tggcgatcaa agacatggcc ggactacttc gtcccgcggc ggcgcgcacc
     2581 ctggtgagtg cgttgcgggc caacttcgac ctaccggtgc atgtgcacac ccacgacacc
     2641 ccgggcggtc agctggccac ctacttggcg gcgtggatcg ccggagccga cgccgtggac
     2701 ggggcgtcgg cggcgatggc cggcggtacc agccagcccg cgctgtcgtc aattgtggct
     2761 gcggcagcac acaccgaccg cgacagcggg ctgagcctgg acgcggtgtg tgacctcgaa
     2821 ccgtactggg aggcgctgcg taaggtgtac gcgccgttcg agtcgggcct gccggcaccc
     2881 accggtcgcg tgtaccacca cgagatcccc ggaggtcagt tatccaacct gcggcagcaa
     2941 gcgattgcgc tcggcctcgg cgaccggttc gaggagattg aagccaacta cgccgccgcg
     3001 gatcgcctgc tggggcggct ggtgaaggtc accccgtcat cgaaggtcgt cggcgattta
     3061 gcgctcgccc tcgtcggcac gggcgtcgac gtcgacgcat tcgccgaaga cccagaccaa
     3121 tacgacattc ccgactcggt ggtcgggttc ctgcgcggtg aactgggtac ccccgcggcc
     3181 tggtggccgg aacccttccg ctccaaggcg ctacgcgggc gcggcgaagc caaacccgaa
     3241 acgccgctgt cggccgtcga cgagacgggt ttggcgggca actcggccga ccgccgcgcg
     3301 acgctgaacc ggttgctatt tcccgggcct accaaggagt tcctcgctca ccggcaaacc
     3361 tacggcgaca cgtcaggcct gtcggccaac cagttcttct atggtctgcg ccgcggcgag
     3421 gagcatcgcg tgcacctgga gaagggtgtc acgttgctga ttgggttgga agccgtctcc
     3481 gaccccgacg agcggggcat gcgcacggtc atgtgcatcc tcaacggcca gctgcggccg
     3541 gtgtcggtgc gcgaccgctc gatcgccagc gacatccccg ccgccgagaa agccgacaag
     3601 accaaccgcg gccacatcgc cgccccgttc gccggggtgg tcaccctgac ggtcaccgag
     3661 ggcgacaagg tcaacgccgg cgaaccgatc gccaccatcg aagcgatgaa gatggaagcc
     3721 gcaatcaccg ctccccgggg cggcctgatc agccgggtcg ccatcgcccc cgtccaacag
     3781 gtcgaaggcg gcgacctcct cgccgccgtc accgtcgccg aggaaaccgg tcccgaatga
     3841 ga
//
LOCUS       DASRJA010000470         8242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1741130,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000470 DASRJA010000000
VERSION     DASRJA010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741130"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..771)
                     /locus_tag="VFC01_20755"
     CDS             complement(52..771)
                     /locus_tag="VFC01_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82090.1"
                     /translation="MNDSLVNTNGAVGRPPQAGPPLAPLAAISLVLLLAGLVVGVALA
                     GGVMPLPYGSATEIQNYVAGNHASAVAIAVGTFGSSIPLAIYAATASARLRQLGITAP
                     GATIALAGGLLASAGLALSSLTAWTLSRPEVTADPALVRALYYLTYLTGGPWHVVTLG
                     LLIAGIAVPGLIVGLLPRAVAWAGLVIAGLAELTTLVLIWPGLSPLLPVARFTGLVWL
                     IVAGVLLPLRRRNKQEVTARS"
     gene            860..1333
                     /locus_tag="VFC01_20760"
     CDS             860..1333
                     /locus_tag="VFC01_20760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZN82091.1"
                     /translation="MSTSGDNVAFLLSQLGHRSASVFAELIASIDLTPPHSGILRAIA
                     SEPGRSQQALSGQLGLLPSRVVAYVDELEDRGYVERRRNPEDRRLHALYLTASGKKIM
                     SKIGELGRQHEKVMTAGLNAEQRDTLRQLLSIVADHQGLTPHVHPGYRTLGRVKP"
     gene            complement(1405..2259)
                     /locus_tag="VFC01_20765"
     CDS             complement(1405..2259)
                     /locus_tag="VFC01_20765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82092.1"
                     /translation="MLPFIGSEGLAAGVVNRYQLGTRYDAVYRDVYVPKGQALTAADR
                     AVAAWLWSRRRATAAGLSAAALHGTLWLDAKLPAELNQRQRHKTDGIVLHSDKLADDE
                     VCIANGVAATTPARTAYDLGRRRGLTTAVIRLDALMQATNLKPADVEALAERHRGARG
                     VVQLREAIELSDSGAESPQETRTRLILTRAGLRPQRTQIEVFDEFGEFVHRIDMGWDD
                     WMVGVEYDGAQHWTDADIRANDIETQARLEALGWRIVRVSADMVRYRPNIIVARTRAA
                     LRTAGAPA"
     gene            complement(2368..3207)
                     /locus_tag="VFC01_20770"
     CDS             complement(2368..3207)
                     /locus_tag="VFC01_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZN82093.1"
                     /translation="MWRLRVVHSTGYAYKSPVTASFNEARLTPRSDSRQNVILNRVET
                     VPATRSYRYVDYWGTAVTAFDLHAPHTELEVTSSSVVETDRDEMPKDKVSWDDLRSEA
                     VIDRFDEVLSGTHYTPVSKRFRRVGERIAKYHDPQEAVTAAANWVHSELEYVPGTTGV
                     HSSGLDAHREGKGVCQDFAHLTLILLRSMGIPARYVSGYLHPKGKAKVGDTIDGQSHA
                     WVQAWTGGWWSYDPTNDKEINEQYVSVGVGRDYSDVSPLKGIYSGEGSTDLDVVVEIT
                     RLA"
     gene            complement(3208..4185)
                     /locus_tag="VFC01_20775"
     CDS             complement(3208..4185)
                     /locus_tag="VFC01_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HZN82094.1"
                     /translation="MLARNAESLYWIGRYVERADDTARILDVTVHQLLEDSSVDPDQA
                     SRTLLRVLGIEAPKEQLDVWSLTDLVAFSRDTAGGCSIVESISAARENARGAREVTST
                     EIWECLNTTYNALAERERAAKRLGPHEFLSYVEGRAAMFAGLADSTLSRDDGYRFMVL
                     GRAIERVDMTVRLLLSRVGDSASSPAWVTLLRSAGAHDTYLRTYRGTMDAGRVVEFML
                     LDRLFPRSIFYSLRLAEHSLDELMKRPHNRLGATAEAQRLLGRARSELEFLRPGLLLD
                     SLEKRLAGLQETCRDVGEALAVQYFHSAPWVAWHDAGHNGALVIEEGEV"
     gene            complement(4200..5765)
                     /locus_tag="VFC01_20780"
     CDS             complement(4200..5765)
                     /locus_tag="VFC01_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HZN82095.1"
                     /translation="MFDADGNVRGPYKGIFTELAPSDASELAARADALGRAFIDQGIT
                     FSLSGQERPFPLDLVPRVISAAEWTRLERGITQRVKALEHYLDDIYGEQEILRDGVIP
                     RRLVTSCEHFHREAAGIMPPNGVRIHVAGIDLIRDAKGDFRVLEDNLRSPSGVSYVME
                     NRRTMARVFPNLFATHRVRAVGDYSSHLLRALRNASASNVADPTVVVLTPGVYNSAYF
                     EHSLLARQMGVELVEGRDLFCRDNAVYMRTTEGERQVDVIYRRIDDAFLDPMQFRPDS
                     VLGVAGLLNAARAGNVVISSAVGNGVGDDKLVYTYVPTIIEYYLGEKPLLANVDTYRC
                     WLDDEREEVLDRVDELVVKPVEGSGGYGIVFGPDASEKELAAISKKIRNDPRGWIAQP
                     VMQLSTVPTQIDDKLSPRHVDLRPFAVNDGDQVWVLPGGLTRVALPEGSLVVNSSQGG
                     GSKDTWVLASRASVADRELAAAEVVRSLPKSTKGAKTDNGSDGSSQQQQQQQSQAQRP
                     TTGSSQQQQQQQQ"
     gene            6055..6315
                     /gene="rpsT"
                     /locus_tag="VFC01_20785"
     CDS             6055..6315
                     /gene="rpsT"
                     /locus_tag="VFC01_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZN82096.1"
                     /translation="MANIKSQEKRNRTNERRRLRNKSVKSSLHTAVRGFREAVEAGDK
                     EKAAELLASTSRKLDKAVSKGVIHKNQAANRKSGLATALNNL"
     gene            complement(6316..7278)
                     /gene="holA"
                     /locus_tag="VFC01_20790"
     CDS             complement(6316..7278)
                     /gene="holA"
                     /locus_tag="VFC01_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZN82097.1"
                     /translation="MSEEVAGLHLVLGDEELLVERAVGAVLRQARKQAGTDDVPVDRL
                     RAGEVSTSELAELLSPSLFADERVVVLEAAGEAGKDAAALIADAAADLPPGTMLVVVH
                     SGGGRAKALADQLKKLGAQVHPCARIAKASERADFVRKEFRALKVKVGDDTVTAVLDA
                     VGSDIRELAAVCSQLVADTDGAVDAVAVRRYHSGKAEVKGFDIADKAVVGDVAGAAEA
                     LRWAMMSGEPQVVLADALAEAVHTIARVGPLSGDPYRLAAELGMPPWRVQKAQKQARR
                     WSRGSIAEAMRLVATLNADVKGAAADADYALEAAVRKVAELAAD"
     gene            complement(7275..>8242)
                     /locus_tag="VFC01_20795"
     CDS             complement(7275..>8242)
                     /locus_tag="VFC01_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZN82098.1"
                     /translation="AQDIRAAFADAARVALPADQAAMLPALVLGDTSTLTAQTTEEFR
                     VAGLTHLTAVSGANVTIVCGAVLMTVALVGPRVAVVLAALALVGFVVVVQPSASVLRA
                     AVMGGITLLAVLSHRRRQAIPVLSACVLMLMIAAPELAVDVGFALSVSATAALVVIAP
                     VWSRRLVGRGWPKPMADAVSVAVAAQLVTAPLVAGISGRFSVVSVVANLAVAVVIPPI
                     TVVGTAAAALCPLWPSGAQLLIRFTGPELWWLLHAAQWAAGVPGASLGVPSGLLGVVC
                     VAAVGVVAVLAWRWRWVRIGTGCAALCLLAWTVSGLSAGHDTIVR"
BASE COUNT         1453 a         2811 c         2603 g         1375 t
ORIGIN      
        1 ggtcgcggat cgagtcgggc cacgagtaat tcgtcacgct cacactgagt ttcatgatcg
       61 agccgtcacc tcctgtttgt tgcgtcggcg cagcggcagg agcacaccgg cgacgatcag
      121 ccagaccagt ccggtgaacc gggcgaccgg caacagcggc gataggccgg gccagatcag
      181 caccaatgtc gttagttcgg cgagacccgc aatcacgagg ccggcccatg cgaccgctct
      241 gggcagcagg ccgacgatga ggccgggcac tgcgatgccc gcgatcagaa ggccgagtgt
      301 gacgacatgc cagggacctc cggtgaggta ggtgaggtag tacagcgcgc ggacgagcgc
      361 tgggtcggcg gtgacctccg gccgcgacag agtccatgcc gtcaggctcg atagtgcgag
      421 cccggccgac gcgaggagcc ctccggcgag tgcgatcgtc gcccccggcg cggtgatgcc
      481 gagctgacgc agccgtgcgc tggcggtggc cgcgtagatg gccagcggta tcgacgaccc
      541 gaacgtcccg accgcaatcg cgacggcgga ggcgtggttg cccgcgacgt agttctggat
      601 ttccgtagcg gagccgtacg gaagcggcat gacgccaccc gcgagcgcga cccccactac
      661 cagtcctgcg agcagcagca ccagggagat cgctgcaagc ggcgccaaag gcgggccagc
      721 ttgcggcggt cggccaaccg cgccattcgt attgactaat gaatcattca taatttgaac
      781 gatatgcctg atcttgaaca tagacaaggt gattcactaa tttgtctgag caatcgttca
      841 cgaatgctac gatttatccg tgtcaaccag cggtgacaat gtggcgttct tgctgtccca
      901 actcggccat cgttctgcct cagtgttcgc ggagctgatc gcgtcgatcg acctcacccc
      961 accgcactcc gggattctgc gtgcgatcgc atccgaaccc gggcgcagcc agcaggcgct
     1021 cagcggccag ctgggtttgt taccgagccg ggtggtcgcc tatgtcgacg agctggagga
     1081 ccgtggctat gtcgaacgtc gtcgcaatcc cgaggaccgg cggctgcacg cgctctatct
     1141 gaccgcgtcg ggcaagaaga tcatgagcaa gatcggcgag ctgggccgtc agcatgaaaa
     1201 ggtgatgacc gcggggctca acgcagagca acgggacacg ttgcgccaac tgctgtcgat
     1261 cgtggccgac catcaaggcc tgacgcccca tgtgcacccg ggttaccgca ccctgggccg
     1321 cgtgaagccg taacaactcg cgccgaacgt tccttaccgc tcgaaatttc gccaagatct
     1381 caagcggtaa ggaacgttcg gcggctaggc cggggcgccg gcggtgcgca gcgcagctcg
     1441 ggtacgcgca acgataatgt tcggccggta ccggaccata tcggcgctga cgcggacaat
     1501 ccgccatccc agcgcctcta gtctggcctg cgtctcaatg tcgttggcgc gtatgtcggc
     1561 gtcggtccag tgttgggcgc cgtcgtactc gacgccgacc atccagtcat cccagcccat
     1621 gtcgatgcga tgcacgaatt cgccgaattc gtcgaatact tcgatctgtg tccgctgtgg
     1681 ccgtaaacct gcgcgcgtaa ggataaggcg ggtccgggtt tcctgcgggg actccgcccc
     1741 actgtccgac agctcgatcg cttcgcgcaa ctgcacgacg ccgcgtgcgc cgcgatggcg
     1801 ctccgccagc gcttcgacat ctgcaggctt gagattggtc gcttgcatca acgcatccag
     1861 tcggatcacc gcggtggtca ggccgcgccg tcggccaagg tcgtatgcgg tgcgcgcagg
     1921 tgtggtcgcc gctacgccgt tggcgataca cacctcgtca tcggccagct tgtcgctgtg
     1981 taacacgatg ccgtccgtct tgtggcgttg ccgttggttg agctcggcgg gtagtttcgc
     2041 gtcaagccaa agggttccgt gcaaggcagc tgcggataac cccgccgcgg tggcccgcct
     2101 tcttgaccac agccaggctg cgaccgcccg gtcggctgca gttagcgcct gacccttggg
     2161 cacgtacaca tcgcgataga cggcgtcata ccgggtgcca agctgatagc gattgacgac
     2221 acccgccgcc aacccttcgc tgccgatgaa tggaagcatg gtgatacgac gcgctcagcg
     2281 tcacaccggt tccatcgacg aacgttccgt accgctcgaa aaatccgcgg aatttcgagc
     2341 ggtaaggaac gttcgcggcg gagggcgtta agccagccgg gttatctcca cgacgacatc
     2401 caggtccgtc gagccctcgc cggagtaaat ccccttcagc gggctgacat ccgagtagtc
     2461 acgcccgacc ccgacactga cgtactgctc gttgatctcc ttgtcgttgg tggggtcgta
     2521 gctccaccag ccaccggtcc atgcttgcac ccacgcgtgg ctctgcccgt cgatggtgtc
     2581 accgaccttc gccttgccct tcggatgcag atagcccgac acatatcggg ccggaatgcc
     2641 catgctgcgc aacaggatca gcgtcagatg ggcgaagtcc tgacagacgc ccttgccttc
     2701 gcggtgcgca tccagccccg acgagtgcac accggtagtg cccggtacat attccagctc
     2761 gctgtgaacc cagttggccg cggcggtgac cgcctcttgc ggatcgtggt acttggcgat
     2821 gcgctccccc acccgccgaa acctcttgct caccggcgtg taatgggtcc cgctgagtac
     2881 ctcgtcgaac cgatctatta ccgcctccga gcgcaggtca tcccagctca ctttgtcttt
     2941 gggcatttca tcgcggtcgg tctccactac cgagctcgag gtgacctcaa gttcggtatg
     3001 cggcgcgtgc agatcgaatg cggtcaccgc ggtgccccaa tagtcgacat agcggtacga
     3061 ccgtgtggca ggaacagttt cgacgcggtt gaggatgacg ttctggcggg aatccgatcg
     3121 tggcgtcaac cgggcctcgt tgaaggatgc ggtcaccggc gacttgtagg cgtagcccgt
     3181 cgaatgcacc accctaagcc gccacatcta gacctcacct tcctcgataa ccagcgcacc
     3241 gttgtggccg gcatcatgcc aagccaccca cggtgcggag tgaaagtact gaaccgccag
     3301 cgcttctccg acatcacgac acgtttcctg caggccggcc agccttttct ccaacgagtc
     3361 cagcagcaga ccgggacgca ggaattccag ctcgctgcgc gcccggccca gcaggcgctg
     3421 cgcctctgcc gttgcgccca gccggttgtg cggccgcttc atcaactcgt caaggctgtg
     3481 ctcggccagc cgcagcgaat agaagatcga ccgcgggaaa agccggtcga gcagcatgaa
     3541 ttcgaccacc cggcccgcgt ccatcgtgcc ccggtaggta cgcaggtacg tgtcgtgggc
     3601 gcccgccgac cgcagcaacg tcacccatgc cggtgaggac gcgctgtcac cgacgcggga
     3661 caataacagc ctcaccgtca tgtcgacgcg ttcgatggcg cggcccagca ccatgaatcg
     3721 gtaaccgtcg tcgcgactca gcgtcgagtc ggccagacca gcgaacatgg cggcgcggcc
     3781 ttcgacatac gacaggaact cgtgcggccc aaggcgtttg gcggcgcgtt cgcgctcggc
     3841 caacgcgttg tatgtggtgt tcaggcactc ccagatctcg gtagacgtga cttcccgtgc
     3901 accgcgggcg ttttcccgcg ccgccgagat ggattcgacg atcgagcagc cgcccgccgt
     3961 gtcgcggctg aacgcgacga gatccgtcaa cgaccacaca tccagctgct ccttgggcgc
     4021 ttcgatgccc aacacccgca gcagcgttcg cgacgcctga tccgggtcga cgctggaatc
     4081 ctcgagtagt tggtgcaccg tcacatcgag gatgcgggcg gtgtcgtcgg ctcgctccac
     4141 atagcggcca atccagtaca gcgattcggc gttgcgcgcc aacatcagat catcaccgcc
     4201 tactgctgct gttgttgttg ctgttgactc gagccggtcg tcggccgttg cgcctgcgat
     4261 tgctgctgtt gttgctgctg cgacgacccg tcggagccgt tatcggtctt tgcgccctta
     4321 gtcgacttgg gcagcgaccg tacgacctcg gccgctgcga gctcgcggtc ggccaccgac
     4381 gcgcgcgatg cgagcaccca ggtgtccttc gacccgccac cctgactgga gttgaccacc
     4441 agggacccct caggcagcgc cacccgggtc agcccgccgg gcagcaccca cacctggtcg
     4501 ccgtcgttga ccgcgaacgg acgcaggtcg acgtggcggg gcgagagctt gtcgtcgatt
     4561 tgggttggca ccgtggacag ttgcatcacg ggctgcgcga tccagccgcg agggtcgttg
     4621 cggatcttct tgctgatcgc ggccagctcc ttctcggacg cgtcgggccc gaacacgatg
     4681 ccgtagccgc cggagccctc caccggcttg accaccagtt cgtcgacccg gtcaagcact
     4741 tcctcgcgtt cgtcgtcgag ccagcagcga taggtatcga cattggccag cagcggcttc
     4801 tcacccaggt agtactcgat gatcgtcggc acgtacgtgt acaccagctt gtcgtcgccg
     4861 acaccgttgc ctacggcgct ggagatgacg acattgccgg cacgagcggc gttgagcagc
     4921 cccgcgacgc cgagcaccga gtcgggccgg aactgcatcg ggtccaggaa tgcgtcgtcg
     4981 atgcggcggt agatgacgtc gacctggcgt tcgccttcgg tggtgcgcat gtagaccgcg
     5041 ttgtcgcggc aaaacaggtc gcggccctcg accaactcca cgcccatctg ccgggccaac
     5101 agcgagtgct cgaagtacgc ggagttgtag acgcccggcg tcagcaccac caccgtggga
     5161 tcggcgacgt tactggccga agcgttgcgc agcgcccgca gcaggtgcga ggaatagtcg
     5221 cctaccgcgc gcacccgatg cgtcgcgaac aggttcggga acactcgcgc catcgtgcgg
     5281 cggttctcca tcacgtacga gacgcccgat ggtgagcgca ggttgtcctc gagcacgcgg
     5341 aagtcacctt tggcgtcgcg gatcaggtcg ataccggcga catggatgcg cacgccgttg
     5401 ggaggcatga tgcccgcggc ctcgcggtgg aaatgctcac aggacgtgac gagtcggcgc
     5461 gggatgacgc cgtcccgcag gatctcctgc tcgccgtaga tgtcgtcgag gtagtgctcc
     5521 agcgctttca cccgctgcgt gatgccgcgc tcgagccgcg tccactcggc cgccgagatc
     5581 actcgcggga cgaggtccag cgggaatggc cgctcctggc cggacagcga gaacgtgatg
     5641 ccctggtcga tgaacgcacg gcccagcgca tcggcgcggg ccgccagctc cgacgcgtcg
     5701 gacggagcaa gctcggtgaa gatgcctttg tacggtccac ggacattgcc gtcggcgtcg
     5761 aacatctcgt caaacgcttc cgaatagcgg cccacgttgg tgtagccgtc gaagatgccg
     5821 tcgtaccgtt tggccgtccg cccgttggcg cgcgacgtcc gagcggtctc attgggcagg
     5881 gttcgaaggc tcacccatca catgctgcac caattgacgg gtttcggcag gccagacggc
     5941 ctcgctttgg gagattcggg ggcgcgctgg taccctgaac agtcgctgcc cgccgatagg
     6001 cgcggcgcgg agagactttg agcaatccaa cctagatttc gaaggaatta ccgcgtggcc
     6061 aacatcaagt cgcaggaaaa gcgcaatcgc accaacgagc gccgcagact gcgcaacaag
     6121 tcggtgaagt cgtcgcttca cacggcggtc cgcggattcc gggaggccgt cgaggccggc
     6181 gacaaggaga aggccgccga gttgctggcg tccaccagcc gcaagctgga caaggccgtc
     6241 agcaagggcg tgatccacaa gaatcaggcg gccaaccgca agtcgggtct ggccaccgcg
     6301 ctcaacaatc tctgatcagt ccgccgctag ttcggctact tttctgaccg ccgcctccag
     6361 cgcgtagtcc gcgtcggcag cggctccctt aacgtcggcg ttcaacgtcg cgaccagtcg
     6421 catggcctcg gcgatcgaac cgcgtgacca tcgtcgcgcc tgtttttgcg ccttctgcac
     6481 ccgccacggc ggcatcccca gttcggcggc gagccggtac ggatccccgg acagtgggcc
     6541 gaccctagcg atggtgtgta cggcctcggc cagcgcatcg gccagcacaa cctgcggctc
     6601 accgctcatc atcgcccagc gcagcgcctc ggcggctccg gcgacatcgc cgacgacggc
     6661 cttgtcggcg atgtcgaagc ctttcacctc cgccttgccg gagtggtagc ggcggaccgc
     6721 gaccgcgtcg acggcgccgt cggtgtccgc caccaactgc gaacagacag cggccaattc
     6781 gcgaatgtcg gagccgaccg cgtcgagtac cgcggtgacg gtgtcgtccc cgaccttgac
     6841 cttcaacgcc cggaactcct tgcggacgaa gtcggcgcgc tcgctggcct tggcgatccg
     6901 ggcgcatgga tgtacttgcg caccaagctt tttcaattgg tcggccaagg ccttggctcg
     6961 gcccccgccg gagtgcacga cgaccagcat ggtgcccggc ggcaggtcgg cggcggcgtc
     7021 ggcgatgagc gcggccgcgt ccttacccgc ttccccggcc gcctcgagaa cgacgacgcg
     7081 ctcgtcggcg aacagcgacg ggctgagcag ttcggccagc tcgctggtgc tcacctcacc
     7141 ggcgcgcagc cggtccaccg ggacgtcgtc ggtgcctgct tgcttgcgcg cctgccgaag
     7201 gacggcgccg acagcgcgct cgacgagcag ctcttcgtca ccaagcacca agtgcaggcc
     7261 cgcgacctct tcgctcacct cacgatcgtg tcatgtccgg ccgacagtcc cgacaccgtc
     7321 cacgccagta ggcatagtgc ggcgcagccc gttccgatgc ggacccaccg ccaccgccac
     7381 gccagcactg cgacgacccc gacggcggca acgcacacga cgccgagcag tcccgacggc
     7441 acaccgagcg aagcgcccgg cactccggcc gcccactgtg cggcgtgcag cagccaccac
     7501 agctccgggc cggtgaatcg gatcagcagt tgcgcgcccg acggccacag cggacacaac
     7561 gccgcagccg ccgtccccac gacagtgatc ggcggtatca cgacggccac ggcgaggttg
     7621 gcgaccacag acaccacgct gaaccggccc gatatgccgg caaccagcgg cgcggtgacc
     7681 agctgcgcgg ccaccgcgac acttaccgca tcagccatcg gtttcggcca tccgcgtccg
     7741 acgaggcgtc gcgaccacac cggtgcgatc acgacaagcg ccgccgtcgc cgaaaccgac
     7801 agcgcgaatc cgacgtcgac ggccagttcg ggcgcggcga tcatcagcat cagcacgcac
     7861 gccgacaaca cggggatcgc ctgcctgcgc cggtgcgaca acaccgccaa cagcgtgatg
     7921 ccgcccatga cggccgcccg cagcacactc gccgacggct gcacgacaac gacgaacccc
     7981 accagcgcca gcgcggccag aacgacggcc accctcggcc cgaccagcgc cacggtcatc
     8041 aacacggcac cgcagacgat cgtgacgttg gcgcccgaca ccgcggtcaa gtgggtcaac
     8101 ccggcgactc ggaactcctc ggtggtctgc gcggtcagcg tcgatgtatc gccgagtacc
     8161 agcgccggca gcattgccgc ttgatccgcc ggcagcgcga cgcgtgccgc atcggcgaag
     8221 gcggcgcgga tgtcttgcgc ga
//
LOCUS       DASRJA010000471         4182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2558966,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000471 DASRJA010000000
VERSION     DASRJA010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2558966"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VFC01_20800"
     CDS             <1..716
                     /locus_tag="VFC01_20800"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZN82099.1"
                     /translation="STGIFTNAAKAKGHLDAGAKKVIISAPATDEDITIVLGVNDDKY
                     DGSQNIISNASCTTNCLGPLAKVLNDEFGIVKGLMTTIHAYTQDQNLQDGPHKDLRRA
                     RAAAINIVPTSTGAAKAIGLVLPELKGKLDGYALRVPIPTGSCTDLTAELSKSATADE
                     INAAFKAAADGPLKGILKYYDAPIVSSDIVTDPHSSLYDAGLTKVIDNQAKVVSWYDN
                     EWGYSNRLVDLVALVGKSL"
     gene            720..1934
                     /locus_tag="VFC01_20805"
     CDS             720..1934
                     /locus_tag="VFC01_20805"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZN82100.1"
                     /translation="MAVKTLDDLLAEGVSGRGVLVRSDLNVPLDDNRNITDPGRIIAS
                     VPTLQALADAGAKVVVTAHLGRPKGGPDPQFSLAPVAKALGDKLGRHVQLAGDVVGTD
                     ALARAEGMTDGDILLLENIRFDPRETSKDDAERLALAKQLVELVGDDGAFVSDGFGVV
                     HRKQASVYDVATLLPHFAGQLVAAEIKVLEQLTSSTERPYAVVLGGSKVSDKLAVIEN
                     LATKADSLVIGGGMCFTFLASQGVPVGKSLLEEGMIDTCRKLLEDYGDVIHVPVDIVV
                     ADEFKADSPAETVAADRIPEDKMGLDVGPGSVERFTTLLSNAKTIFWNGPMGVFEFPA
                     FAAGTKGVAEAIIAATAKGAFSVVGGGDSAAAVRQLGLPEDGFSHISTGGGASLEYLE
                     GKTLPGIEVLSD"
     gene            1943..2728
                     /gene="tpiA"
                     /locus_tag="VFC01_20810"
     CDS             1943..2728
                     /gene="tpiA"
                     /locus_tag="VFC01_20810"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZN82101.1"
                     /translation="MSRKPLIAGNWKMNLNHFEAIALVQKIAFSLPDKYFDKVDVAVL
                     PPFTDLRSVQTLVDGDKLRLTYGAQDLSQHESGAYTGDISGAFLAKLGCTYVVVGHSE
                     RRTYHEEDDALVAAKSAAAFKHGLTPIICIGEQLEVREAGNHVEHNVEMLRGSLAGLS
                     ADQIGNAVIAYEPVWAIGTGRVASAADAQEVCKAIRDDLGKLASPELAAGVRVLYGGS
                     VNAKNVGEIVAQEDVDGALVGGASLDGEQFATLSAIAAGGPLP"
     gene            2760..2993
                     /gene="secG"
                     /locus_tag="VFC01_20815"
     CDS             2760..2993
                     /gene="secG"
                     /locus_tag="VFC01_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZN82102.1"
                     /translation="MELALQIILVVTSVLVVLLVLLHRAKGGGLSTLFGGGVQSSLSG
                     STVVEKNLDRLTLFVTGIWLVSIVGVALQIKYS"
     gene            complement(2990..3235)
                     /locus_tag="VFC01_20820"
     CDS             complement(2990..3235)
                     /locus_tag="VFC01_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82103.1"
                     /translation="MKRRVIPAALFAAAMAVALGYPATAAAEPVCPMNTVTIGNQGKV
                     INVPGDSQVCDDDPLGSDGLLSNLPLLGNLPGVSGIL"
     gene            3321..>4182
                     /locus_tag="VFC01_20825"
     CDS             3321..>4182
                     /locus_tag="VFC01_20825"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HZN82104.1"
                     /translation="MADDVTLAPIGSVRRTQVGREATEPMREDIRLLGTILGDTVRDQ
                     NGEEVFDLVERARVESFRVRRSEIDRAELARMFDGIDIHRAIPVIRAFSHFALLANVA
                     EDIHRERRRAVHVAAGEPPQNSSLAATYLKLDSAELDSATVADTLAGALVSPVITAHP
                     TETRRRTVFDTQHRITELMRLRLHGHSQTEDGRDISIELRRHILTLWQTALIRLSRLK
                     IQDEIETGLRYYQAALFEVIPQVNAEVRAALQARWPDAHVLDEPMLRPGSWIGGDRDG
                     NPNVTADVVRL"
BASE COUNT          738 a         1392 c         1368 g          684 t
ORIGIN      
        1 agtccaccgg catcttcacc aacgccgcca aggccaaggg ccacctcgac gctggcgcca
       61 agaaggtcat catttccgcg cctgccaccg acgaggacat cacgatcgtg ctcggcgtca
      121 acgacgacaa gtacgacggc agccagaaca tcatctccaa cgcgtcgtgc accacgaact
      181 gcctcggccc gctggccaag gtgctcaacg acgagttcgg catcgtcaag ggtttgatga
      241 cgacgatcca cgcgtacacg caggaccaga acctgcagga cggcccgcac aaggacctgc
      301 gccgcgcccg cgccgccgcg atcaacatcg tgccgacctc gaccggcgcc gcaaaggcca
      361 tcggcctggt gctgcccgag ttgaagggca agctcgacgg ctacgccctg cgggtgccga
      421 tcccgacggg ttcgtgcacc gacctgaccg ctgagctgtc caagtcggcc accgccgacg
      481 agatcaacgc cgcgttcaag gcggccgccg acggtccgct caagggcatc ctcaagtact
      541 acgacgcgcc gatcgtgtcg agcgacatag tcaccgaccc gcacagctcg ctctacgacg
      601 cgggcctgac gaaggtcatc gacaaccagg ccaaggtcgt ctcctggtac gacaacgagt
      661 ggggctactc caaccgcctc gtcgacctgg tcgcgctggt cggcaagtcg ctctaaccaa
      721 tggccgtcaa gacactcgac gacctactcg cagaaggggt ttcgggccgg ggcgtgttgg
      781 tgcgctccga cctgaacgtc ccgctcgacg acaaccgcaa catcaccgac ccgggccgca
      841 tcatcgcgtc ggtgccgacg ctgcaggcgc tggccgacgc tggcgccaag gtcgtcgtca
      901 ccgcgcacct cggtcggccg aagggcggtc ctgatccgca gttctcgcta gcgcccgtcg
      961 caaaggcgtt gggggataag ctcggccgtc acgtccagct cgccggtgac gtcgtcggaa
     1021 ccgacgcgct ggcccgtgcc gaaggcatga ccgacggcga catcctgctg ctggagaaca
     1081 tccggttcga tccgcgcgag acgagcaagg acgacgccga acggctggcg ctggccaagc
     1141 agctggtcga attggtaggc gacgacggcg ctttcgtctc cgacggcttc ggggtggtgc
     1201 accgcaagca ggcgtcggtc tacgacgttg cgacgctgct gccgcacttt gccggccagc
     1261 tggtggccgc cgagatcaaa gtgctcgagc agttgaccag ctcgaccgag cgaccgtatg
     1321 cggtggtgct cggcggctcg aaggtgtcgg acaagctggc ggtaatcgag aacctggcga
     1381 ccaaggccga cagcctcgtc atcggcggcg gcatgtgctt cacattcctt gcttcacaag
     1441 gtgttccggt gggcaagtcg ctgctcgaag agggcatgat cgatacctgc cgcaagctgc
     1501 tcgaggatta cggcgacgtg atccatgtgc cggtggacat cgtcgtcgcc gacgagttca
     1561 aggccgattc gccggcggaa accgttgcag ccgaccgtat tccggaagac aagatgggtc
     1621 tggacgtcgg ccccggttcg gtcgagcggt ttaccacgct gctgtccaac gccaagacga
     1681 tcttctggaa cggcccgatg ggcgttttcg agttccccgc cttcgcggcg ggcaccaagg
     1741 gcgtcgccga ggcgatcatc gccgcgaccg ccaagggcgc attcagcgtc gtcggcggcg
     1801 gcgattcggc cgcggcggtg cgccaactcg gcctgcccga ggacggcttc tcccacattt
     1861 ccaccggcgg cggggcctcg ctggaatacc ttgagggcaa gacactgcct ggcatcgaag
     1921 ttctgagcga ttaggagatc ccgtgagccg taagccgctg atcgccggca actggaagat
     1981 gaacctcaac cacttcgagg cgatcgcgct ggtccagaag atcgcgttct cgctgccgga
     2041 caagtacttc gacaaggtcg acgtcgccgt gctgccgccc ttcaccgacc tgcgcagcgt
     2101 gcagaccctg gtcgacggcg acaagctgcg gctcacctat ggcgcccagg atttgtcgca
     2161 gcacgaatcc ggtgcctaca cgggcgacat cagcggtgcc ttcctggcca agctgggctg
     2221 cacgtacgtc gtcgtcggcc actcggagcg gcgcacctac cacgaggagg acgacgcgct
     2281 cgtcgccgcc aagtcggcgg ccgcgttcaa gcacggcctg accccgatca tctgcatcgg
     2341 tgagcaactc gaggtgcgtg aggcgggcaa tcacgtcgaa cacaacgtgg agatgctccg
     2401 gggctcgctg gctgggctgt cggccgacca gatcggcaac gcggtgatcg cctacgagcc
     2461 ggtgtgggcc atcggcacgg gccgggtggc aagcgcggcc gacgcacaag aggtctgcaa
     2521 ggccatccgc gacgatctgg gcaagttggc gtcaccggag ctcgccgcag gcgtccgcgt
     2581 gctgtacggc ggctcggtca acgccaagaa cgtcggcgaa atcgtcgccc aggaagacgt
     2641 ggacggcgcg ctggtcggcg gcgcgtcact ggacggggag cagttcgcca cgctgtcggc
     2701 catcgcggcc ggcgggcccc tgccctgacc acggcggatc attaggtagt ctggcggcca
     2761 tggaattggc cctgcagatc atcctggtcg tgaccagtgt gctggtcgtg ctcctggtgc
     2821 tgctgcatcg cgccaagggc ggcggcctgt ccacgctgtt cggcggcggc gttcagtcca
     2881 gcctgtcggg gtcgaccgtg gtggaaaaga accttgaccg cctgacgctg ttcgttaccg
     2941 gtatctggct ggtttcgatc gtcggcgtcg cgctgcagat caagtacagc tagaggattc
     3001 cgctgacgcc gggcaggttc ccgagcagcg gcagattgct caaaagcccg tcgctgccca
     3061 gcgggtcgtc gtcgcatacc tgggaatccc ctggcacgtt gatgactttc ccctgattgc
     3121 cgattgtgac ggtgttcatc gggcagaccg gctcggcggc tgcggtggcc gggtagccga
     3181 gtgcgactgc catcgcggcg gcgaagagcg ctgcgggtat aactcgacgc ttcatgaggc
     3241 cccctaggtt ttgtttgcta gtcaccagca tgcgcttcgc cagcaggaca gggcaagagc
     3301 ccgcgccgat acttgggggc atggcagacg atgtcaccct ggcaccgatc ggttcggtgc
     3361 gccgcaccca ggtgggtcgc gaggcgaccg agccgatgcg ggaagacatc cggctgctcg
     3421 gcaccatcct gggcgacacc gtccgcgacc agaatggcga ggaggtgttc gacctcgtcg
     3481 aacgggcccg cgtcgagtcg tttcgggtgc gacgctccga gatcgatcgc gccgagctgg
     3541 cgcgcatgtt cgacggcatc gatatccatc gggccattcc cgtcatccgg gcgttctctc
     3601 acttcgcgct gctcgccaac gtcgccgagg acatccatcg cgaacgtcga cgggccgtgc
     3661 atgtcgcagc aggcgagccg ccacagaaca gcagcctggc cgctacatat ctgaaactcg
     3721 actccgccga actggattcg gcaacggtcg ccgataccct ggccggtgcg ctggtgtcgc
     3781 cggtcatcac tgcgcatccc accgagacgc gtcggcgcac ggtattcgac acccaacacc
     3841 ggatcaccga actgatgcgg ctgcgactgc acgggcacag ccagaccgaa gacggccgcg
     3901 acatcagcat cgagctgcgc cgccacatcc tcacgttgtg gcagaccgcg ctgatccggt
     3961 tgtcgcggtt gaagattcag gacgagatcg aaaccggcct gcgttactac caggccgcgc
     4021 tcttcgaagt catcccacag gtcaacgcgg aagtgcgcgc cgcgctgcag gcccgctggc
     4081 ccgatgcgca tgtcctcgac gagccgatgc tgcggcccgg ttcgtggatc ggcggagacc
     4141 gtgacggcaa tcccaacgtc accgccgacg tcgttcggct cg
//
LOCUS       DASRJA010000472         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1095530,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000472 DASRJA010000000
VERSION     DASRJA010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095530"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..1108)
                     /locus_tag="VFC01_20830"
     CDS             complement(329..1108)
                     /locus_tag="VFC01_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82105.1"
                     /translation="MLDLEPHGPLPSQIYWRRRALALGIAVLVVGVIAAVVVMVVMNS
                     TGTEKKAADKPNPTQAAAPTPLPGENPEVKTPVMPPAQQAPAPTPTPTAAVSPPPVLK
                     EGDDCPDSTLAVKGITNQPQYVVGEQPKFTMVVTNIGLVACQRDVGAAVLAAYVYSLD
                     NTRLWSNLDCAPSNETLVKTFQPGEQVTTEVTWTGMGSAPQCPLPRQPIGPGTYNLVV
                     QLGNLRSATVPFILAPPTAPAQAPPAEGSPPAGPAPAPQGG"
     gene            complement(1168..1788)
                     /locus_tag="VFC01_20835"
     CDS             complement(1168..1788)
                     /locus_tag="VFC01_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZN82106.1"
                     /translation="MPNTNPVTAWKALKEGNERFVAGKPEHPSQSIEHRASLAEAQKP
                     TAVVFGCADSRVAAEIIFDAGLGDMFVVRTAGHVIDSAVLGSVEYAVTVLNVPLIVVL
                     GHDSCGAVKATLAALDDGAVPGGYVRDVVERVTPSILLGRRDGLTRVDEFEARHVTET
                     GAQLCNRSTAIAKRIGDGSLAIVGVTYQLADGRVVLRDHLGDIGEG"
     gene            1787..>2333
                     /locus_tag="VFC01_20840"
     CDS             1787..>2333
                     /locus_tag="VFC01_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZN82107.1"
                     /translation="MADIVQEYQAVIDSRELSRWYEHAQRDLPWRRPGVTAWQILVSE
                     FMLQQTPVARVEPIWLAWVARWPTPSATAAASAADVLRAWGKLGYPRRAKRLHECATV
                     IAELFDDVVPSDVETLLTLPGVGAYTARAIACFAYQQPVPVVDTNVRRVVARAVHGRA
                     DAATSSPRDLDDVAALLPNDAG"
BASE COUNT          348 a          777 c          809 g          399 t
ORIGIN      
        1 ccagagacct agggcacttc aacgtcaccc gaggcccagc aaagtggtcg cgcccaccgt
       61 tttgtccgat gggccttaga acagtaatga ccctcccgtc gtggagcgcg gtcctgcaga
      121 cgggtgtgac tcccttgctg cgcaacacct ttcatcgatg acgtcggaat cggtcatcgg
      181 cacgttgatg atgcaggcga tcgcaggagc ccagtcgtca tcgtctgaag cctcgatgac
      241 gaagcgccct gtggccgctc gggtccggct aacggccgcg agtccaccgc cgatgaaaat
      301 gtgcccgcgc ccgggccgcc ctcgtcggct agccgccctg cggcgcgggt gcaggtccgg
      361 ccggcggact gccttcggcc ggcggcgcct gagcgggcgc ggtcggtggc gcgaggatga
      421 acggcaccgt cgcggatctc agattgccta gttgcacgac gaggttgtac gtgcctggcc
      481 cgatcggttg tcgcggcagc gggcactgcg gggcagaacc catgcccgtc caggtcacct
      541 ccgtcgtcac ctgctcgcct ggctggaacg tcttgaccaa tgtctcgttc gacggggcgc
      601 agtcgaggtt cgaccacagc cgggtgttgt ccagcgagta gacgtaggcg gcgagcaccg
      661 ccgcgccgac gtcacgctgg catgcgacga gaccgatgtt ggtcacgacc atggtgaact
      721 tcggctgctc gccgacgacg tactgtggct ggttggtgat gcccttgacc gcaagcgtgg
      781 agtcggggca gtcgtcaccc tccttgagca ccggcggcgg cgagacggcc gcggtcgggg
      841 tgggcgtcgg cgccggggcc tgctgagcgg gcggcatcac cggggtcttg acctcggggt
      901 tttccccggg cagcggggtg ggcgcggcgg cctgcgtggg gttgggcttg tcggcggcct
      961 tcttctcggt gcccgtgctg ttcatcacga ccatcacaac caccgcggcg atgacgccga
     1021 cgacgaggac cgctatgccc agcgcgagtg ccctgcgtcg ccagtagatc tgcgacggta
     1081 gcgggccatg cggttctaga tccagcacaa gctcacggta agggcaggtc acgccgcctc
     1141 gtccgaggtg gctcggcgtg tcgccggtca gccttcgccg atatcgccca gatgatcgcg
     1201 caacaccacc cggccgtcgg ccagttggta ggtgaccccg acgatcgcca gcgagccgtc
     1261 gccgatgcgc ttggcgatgg ccgtggagcg attgcacagc tgggcaccgg tctccgtgac
     1321 gtgcctggcc tcgaactcgt cgaccctggt caacccgtcg cggcggccca gcaggatcga
     1381 cggcgtcacg cgttcgacca catcgcgcac gtagccgccg ggcaccgcgc cgtcgtccag
     1441 cgcggccagc gtcgccttca ccgcgccgca gctgtcgtgg cccagcacca cgatcagcgg
     1501 gacgttgagc accgtcaccg cgtattcgac cgaacccaga acggcggagt cgatgacgtg
     1561 gcccgcggta cgcaccacga acatgtcgcc caagcccgcg tcgaagatga tctcggcggc
     1621 cacccgacta tcggcgcagc cgaacaccac cgcggtgggc ttctgcgcct cggcgaggct
     1681 ggcccgatgc tcgatgcttt ggctgggatg ttcgggcttg cccgcaacga agcgctcgtt
     1741 accctccttg agtgctttcc acgcggtcac cggattcgtg ttgggcatgg ccgacattgt
     1801 gcaggaatat caggctgtga tcgattcacg tgagttatca cggtggtacg agcacgccca
     1861 gcgtgacctg ccgtggcgtc gacccggcgt gacagcgtgg cagattcttg tgagcgagtt
     1921 catgcttcag cagacgccgg tggcacgcgt cgaaccgatc tggctggcgt gggtggcccg
     1981 ctggccgaca ccgtccgcga ccgccgccgc cagcgctgcg gatgtgctgc gggcctgggg
     2041 caagctcggg tatccgcgcc gggccaagcg gctgcatgaa tgcgcgacgg tgattgccga
     2101 gctgttcgac gatgtggtgc cgtcggatgt cgagacgttg ttgacgctgc ctggcgtcgg
     2161 cgcctacacc gcccgggcaa tcgcctgctt cgcctaccag cagccggtgc ccgtcgtcga
     2221 cacgaacgtg cggcgagtgg tggcgcgggc cgtgcacggc agggccgacg cggcaacgtc
     2281 atcaccgcgc gacctcgacg acgtcgccgc gttgttgccc aatgatgctg ggg
//
LOCUS       DASRJA010000473         3563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2543726,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000473 DASRJA010000000
VERSION     DASRJA010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543726"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="VFC01_20845"
     CDS             complement(<1..1490)
                     /locus_tag="VFC01_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82108.1"
                     /translation="MDIVLGVSMTPTTVRMVLVEGEKADGSIVDHDLFEVAANEGSAT
                     SSNAAEQVVEAILGTKESAAAGGHHLKSIGVTWSDHAEASTLRDALSARGVDDVMLVS
                     DGHAAGALAQAAGRAVGYATTALLFIDRDTATLSVVQTDDGSVVKVLSQSLHSDDAMA
                     VLTEMAAAVDAQDSPPQGMFVVGSGVDVTSVKAHLATLVSLPVSAPDEPELALARGAA
                     LASANAPTFEATTVGLAYSQDPDGTTAGNAYVGLAAAETQLAPVSGEDYAAQTDLEPI
                     AAEEGRKPFLLVGSALTSIFVVGVVALVISLAVSIRPTVDQRPAPGESAIVPSTVAPA
                     PAPVQEAQQPVAPPPPAETIKPPVPVAVQEAPAPKQAPRQVFVEQAPAPQAPAPVPEA
                     PPLPPAAPPPAPIPVAPAPVPYIPAPVYNPPVYNPYLPPPVYIPRPRPWQPPWTPPWQ
                     QPQPQQPPQQDPPDDPPTHQNPPPWQWPGSGGGSGGSGSGGSGSGGS"
     gene            complement(1706..3019)
                     /locus_tag="VFC01_20850"
     CDS             complement(1706..3019)
                     /locus_tag="VFC01_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZN82109.1"
                     /translation="MPEIDPAATAWLLASTALVLLMTPGLAIFYGGMVRTTGVLNMIM
                     MSFISIPLVTVAWLLVGYTIAFSDDSAGGVVGSLKHIGMLGINPDTARGPVPELLFAT
                     FQLGFAIVTAALVSGAIADRAKFAAWMVFVPLWTVAVYAVVAHWVWAPGGWLFELGAL
                     DYAGGLVVEIVSGMSALALALVLGPRIGFKTDAMRPHNLPFVLLGCGLLWFGWFGFNA
                     GSALAANGLASAVFLNTLVAGCLGMLGWITVEQIRDGRPTTFGAASGVIAGLVAITPS
                     CGTVNTLGAAVVGLAAGIVCSFAVGAKFRFGYDDSLDVVGVHFVGGVVGVLLIGFLAT
                     EVMTGGANGLFYGGGLTQLGKQALGAVVVGAYAFVVSYVLAKVIDRLMGFRISAEDET
                     TGVDFTQHAETAYAEAVHGHQPSRRPMSLGDLSAPAPRPEKAEEA"
     gene            complement(3154..>3563)
                     /gene="dcd"
                     /locus_tag="VFC01_20855"
     CDS             complement(3154..>3563)
                     /gene="dcd"
                     /locus_tag="VFC01_20855"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZN82110.1"
                     /translation="QQDELTSLVQPDPGEPFVLHPGEFVLGSTLELCTLPDDLAGRLE
                     GKSSLGRLGLLTHSTAGFIDPGFSGHITLELSNVANLPITLWPGMKIGQLCLLRLTSP
                     AEHPYGSAQAGSKYQGQRGPTPSRSYQNFIKSG"
BASE COUNT          620 a         1182 c         1231 g          530 t
ORIGIN      
        1 gagccacccg agccggaacc acccgaacct gatccacccg agcccccgcc ggatcccggc
       61 cactgccacg gcggcgggtt ctgatgtgtc ggcggatcgt ccggcgggtc ttgctgcggc
      121 ggctgctgcg gctgcggctg ctgccacggc ggtgtccacg gcggttgcca cggcctgggc
      181 cgcgggatgt agaccggcgg cggcagatac gggttgtaca ccggcgggtt gtacaccggc
      241 gccgggatat atggaaccgg cgctggcgcc accggaatcg gagcgggcgg cggcgccgcg
      301 ggaggcagcg gtggcgcttc gggtacaggc gcgggtgcct gcggggcggg cgcctgctcg
      361 acgaagacct gacgcggcgc ctgcttgggc gcgggcgctt cctgcacagc gacgggcacc
      421 ggcggtttga tggtctccgc gggtggcggc ggtgcgacgg gctgctgcgc ttcctgcacc
      481 ggcgcgggcg caggtgccac ggtgctcggc acgatcgcgc tctcgccagg ggccggccgc
      541 tggtcgaccg tcgggcgaat gctgacagca agcgaaatca caagcgcgac aacaccaacc
      601 acgaagatcg acgtcagtgc actgccgacg agcagaaacg gcttgcggcc ttcctcggcg
      661 gcaatcggtt cgagatccgt ctgcgcggcg taatcctcac cgctgaccgg agccagctgc
      721 gtttccgcgg cggcaaggcc gacgtatgcg ttaccggcgg tggtgccgtc ggggtcctgg
      781 gagtaggcca gcccgactgt ggtggcctcg aacgtggggg cgttggcgga tgccagcgcg
      841 gcgccgcggg ccagcgcgag ttcgggttcg tcgggtgcac tgaccggcag gctgacgagg
      901 gtcgccaggt gggccttcac cgaagtgacg tcgactcccg agccgacgac gaacatgccc
      961 tgcggcggcg aatcctgggc gtcgacggca gcggccatct cggtcaggac ggccatcgcg
     1021 tcgtcactgt gcagggactg gctcaacacc ttgacgacgg agccgtcgtc ggtctgcacc
     1081 accgacagcg tcgcggtgtc ccggtcgatg aacaacaacg ccgtggtggc gtacccaacg
     1141 gcccggcctg cggcctgagc cagcgcaccg gcggcatggc cgtcggacac caacatcacg
     1201 tcgtcgacgc cgcgcgcgga cagcgcgtca cggagcgtcg acgcctcggc gtggtcgctc
     1261 cacgtgacgc cgatcgactt caagtggtgg ccgcccgcgg cggcgctttc cttggtgcca
     1321 agaatggcct cgacgacctg ctcggccgcg ttcgaagacg ttgctgagcc ctcgttggcg
     1381 gcgacctcga agaggtcgtg gtcgacgatc gacccgtctg ctttctcgcc ctcgaccagc
     1441 accatgcgga ccgtcgtagg tgtcatcgag acccctaata cgatgtccac tgaccctcca
     1501 aagtgtttgc tacgctatct agatctctct ataggcctca ggtcacagga gccgacgacc
     1561 cgatatttca tcgccccgga cagcatggcc gttacattcg acggcgttag ctgacaacag
     1621 tcgccggcac cggtatcaaa aaccctactc ggcttaacga caaccggcgc cggacggttc
     1681 acattccctt acgccgccga acgattcacg cttcttcggc cttctccggt cgcggcgcgg
     1741 gggcgctgag gtcaccgagc gacatcggcc ggcgcgacgg ctgatgcccg tgcaccgcct
     1801 ctgcgtacgc ggtctccgcg tgctgcgtga agtcgacacc ggtcgtctcg tcctcggcgc
     1861 tgatccggaa gcccatcaac cggtcgatca ccttggccaa cacataggag acaacgaacg
     1921 cgtacgcgcc gacgaccact gctccgagcg cctgcttgcc gagctgggtg agtccgccgc
     1981 cgtagaacag gccgttggcg ccgccggtca tcacctcggt ggcgaggaat ccgatcaaca
     2041 gcacgccgac gacgccgccg acgaagtgca caccgaccac gtcgagtgag tcgtcgtagc
     2101 cgaaacggaa tttcgccccg acggcgaatg agcagacgat gcccgccgcc agcccgacaa
     2161 ccgcggcgcc gagggtgttc acggtcccgc aggacggggt gatggcgacc aggccggcga
     2221 tcacaccgga ggcggcgccg aacgtcgtcg gcctgccgtc gcgaatctgc tcgacggtga
     2281 tccaccccag catgccgagg caaccggcca ccaacgtgtt caagaagacc gccgacgcca
     2341 acccgttggc ggccaacgcg gaaccggcgt tgaagccgaa ccagccgaac cacagcagac
     2401 cgcagccaag caagacgaac ggcaggttgt gcgggcgcat ggcgtccgtt ttgaagccga
     2461 tccggggacc gagcaccaga gccagcgcga gggccgacat gccggagacg atttcgacga
     2521 ccaggccgcc cgcgtagtcc agcgcgccta gctcgaacag ccagccgccc ggcgcccaga
     2581 cccagtgggc caccaccgcg tagacagcga ccgtccacag cgggacgaac accatccagg
     2641 ccgcgaactt ggcgcggtcg gcgatcgcac cgctgaccaa cgcggcggtg acgatcgcga
     2701 agcccaattg gaaagtggcg aacaacaatt cgggcaccgg cccgcgggcg gtgtcgggat
     2761 tgatgccaag catcccgatg tgtttgaggc tgccgacgac tccgcccgcg ctgtcgtcgg
     2821 agaacgcgat ggtgtagccg accagcagcc acgccacggt caccagcggg atcgagatga
     2881 agctcatcat gatcatgttg agcacgccgg tggtgcggac catgccgccg tagaagatgg
     2941 cgaggcccgg ggtcatcagc aggacgagtg cggtgctggc gagcagccat gcggtggcgg
     3001 caggatcgat ctctggcatt cgggccccct aacggtcccg aagagcataa gtgccgcgtt
     3061 tcggccgagt taccgaaacc tcacggtccc gtttgtcggc gagtggccac ttgtcgcacg
     3121 caaattccgt tgaagcgtgt cataactggc ctgtcagccg gacttgatga agttctgata
     3181 cgagcgcgat ggggtcgggc cacgctggcc ctggtacttg gagccggcct gcgcactgcc
     3241 gtaggggtgc tcggcggggc tggtcaggcg gagcaggcac agctggccga tcttcatccc
     3301 gggccacagc gtgatcggca ggttggcgac attggacagc tcaagggtga tgtggccgga
     3361 gaaaccgggg tcgatgaagc cggcggtgga atgggtcagc aggccgagcc ggccgagcga
     3421 cgacttgcct tcaagccggc cggcgagatc gtcgggcaga gtgcacaact cgagcgttga
     3481 gccgagcacg aactcgccgg gatgcagcac gaacggctcg cccgggtccg gctgcacgag
     3541 gctggtgagc tcgtcctgct gga
//
LOCUS       DASRJA010000474         4559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1432219,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000474 DASRJA010000000
VERSION     DASRJA010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432219"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1325
                     /locus_tag="VFC01_20860"
     CDS             159..1325
                     /locus_tag="VFC01_20860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009475548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN82111.1"
                     /translation="MDFELNEDQQTIRAAVTELAGKFDDHYWMTKDQRHEFPTEFYDA
                     FAEGGWLGITTPEEYGGHGFGITEASLLLEEVAASGAGMNGASSMHMSIFGMHPVIVH
                     GSKELKARTLPRIVTGELHVCFGVTEPGAGLDTTKITTFARRDGDRYIVNGRKVWISK
                     AVESEKILLLTRTTKFEDVTRKTDGLTLFLTDLDRSKVDIKPIAKMGRNAVTSNELFI
                     DGLEVPVEDRVGEEGQGFKYILDGLNPERCLIAAEALGIGRAALRAAVRYGNEREVFG
                     RPIGMNQGIQFPLADSLARLDAAELVLRKATWLYDNGKSCAREANTAKYLCADAAFDA
                     ADRALQTHGGMGYSEEYHVARYFREARLTRIAPISQEMILNYLGSHVLGLPRSY"
     gene            1325..2092
                     /locus_tag="VFC01_20865"
     CDS             1325..2092
                     /locus_tag="VFC01_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN82112.1"
                     /translation="MNQVRDVFRLDGRSALVTGAGSGVGEAIARAFSTAGAAVLVTDI
                     DKDAAVAAAESITAAGGVAQPFVLDVRDSAAATEASAAAAELGDGALHILVNNAGAIA
                     PAMFADLSEEKFRRIIDIHLVGSFVCSQAALGHLPTDGTGRIINVTSAAGLTGTIGQA
                     NYGAAKAGIVGLTKSLARELARQSITVNAVAPLAATAMTENIRSNEKLAAKTLARIPL
                     GRWAFPDEIAPTFVFLASDAASYITGQVLPVDGGTVI"
     gene            2092..3294
                     /locus_tag="VFC01_20870"
     CDS             2092..3294
                     /locus_tag="VFC01_20870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZN82113.1"
                     /translation="MAHNVTGPFASTGREVYIAEAVRTPTGKSHPERGWYRDIHPNAM
                     LAACYTELAARSGIEPALVEDLVIGCTAPFGEQSRNIARNAWLQAGYPPEVPATTLDR
                     RCGSAQTAVTMGAALVASGTHDVVIAGGVEHMGHVPIDSPGKVSDLYGDPWPAELHDR
                     YAFVHQGESAELIADRWQLGRDEMDAFAARSHQNAAKAIADGRFVGEMVPMTLDGQLR
                     QADQCVRPETTAETLANLKPVFRKENGRITAGSSSPISDGAAAVLLCSGEAAHRLGLR
                     VRARIVDQTTVGVDPIIMLTGPIPATWKLLQRNNLGIDDIDLFEVNEAFSSVVLAWQR
                     ELLPDVDRVNVNGGAIALGHAVGATGARLMATLVAELDRREADLGLVTMCCGGGLGTG
                     TLIQRVPT"
     gene            3291..4517
                     /locus_tag="VFC01_20875"
     CDS             3291..4517
                     /locus_tag="VFC01_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HZN82114.1"
                     /translation="MIDLGKYITAGAGVWWGQAAAEPRPLVDALITQNPRIGPIRTFT
                     GLSWNDQLAVTMPSRVSMVSYGGLGELRELSRSGRLEVVPCHYSALPRMFAERRLPCD
                     VGLVQVSAPDENGRCSLGIGVDYVADALRHTPVLIAEINQRMPVTVGTPRIPLDRFAA
                     TIITDRPLPEDPTRAPGLTEQRIAAHVAELVDDGDTVQLGVGSLGAAVLDALAVHEDL
                     GFHTGMVTDGLLRLVDKGVATGSRKEIDPGLIVAGTALGSAELYQRVPELPAKFLPTS
                     YTHSPQVLSQLRSLVAVNFAVEVDLSGQVGAEVSRGVYVGAVGGQVDFARAAALTGKR
                     SIIALRATSGGRSTIKVALDGGAVTTSRSDVDAVVTEYGVAHLRGCSIRERAERMIAI
                     AAPEFRDQLDQERTNL"
BASE COUNT          813 a         1537 c         1462 g          747 t
ORIGIN      
        1 gaccgcgtgg aatgagcgga tcgcgcagtc cctgccgctc atccctgttc cggcctgatc
       61 acacatgtga ccagcggtac tcgacgcggc cttccttttt ggtctgtcca ttagtatgat
      121 tcaagcaata agttcccgtt attggagcgg agatcaatgt ggacttcgag ttgaacgagg
      181 atcagcagac gatccgcgcg gccgtcaccg aactggctgg caagttcgac gaccactatt
      241 ggatgaccaa ggatcagcgg cacgaattcc cgacggagtt ctatgacgcc ttcgccgaag
      301 gtggttggct cggcatcacc accccggagg aatacggcgg ccacggcttc ggcatcaccg
      361 aggcatcgct tcttctggag gaagtcgcgg ccagcggcgc cgggatgaac ggcgccagct
      421 caatgcacat gtcgatcttc ggcatgcatc cggtcatcgt gcacggctcc aaggaactca
      481 aggcccgcac gctgccgcgc atcgtgaccg gtgagctaca tgtctgtttc ggcgtcaccg
      541 agccgggcgc gggtctggac accacgaaga tcaccacgtt cgcgcggcgc gacggcgatc
      601 gctacatcgt caacgggcgc aaggtgtgga tctccaaagc cgttgaatcc gagaagatcc
      661 tcctgctgac ccgcaccacc aaattcgaag acgtcactcg caagaccgat ggcctgacgc
      721 tgttcctgac cgacctcgat cgcagcaagg tcgacatcaa gccgatcgcc aagatgggcc
      781 gaaatgcggt gacctccaac gagttgttca tcgacggcct cgaagtccct gtcgaggacc
      841 gcgtcggcga ggaaggccag ggcttcaagt acattctcga cggcctgaac cccgagcgct
      901 gcctgatcgc cgccgaggcg ctcggcatag ggcgtgccgc ccttcgcgcg gcggtccgct
      961 acggcaacga acgtgaggtg ttcggcaggc cgatcggaat gaaccagggg atccaatttc
     1021 cgctggcgga ctcgttggcg cgcctggacg ccgccgaact cgtcctgcgc aaggccacat
     1081 ggctctacga caacggcaaa tcctgtgcac gcgaagccaa tacggccaag tacctgtgcg
     1141 ccgatgcggc attcgacgct gctgatcgcg cattgcagac gcacggcggc atggggtact
     1201 cggaggagta ccacgtcgcc cgttatttcc gcgaggcgag gctgacccgg atcgccccga
     1261 tcagccagga gatgatcctc aactatctgg ggtcgcatgt gctgggcttg ccgaggagct
     1321 actgatgaac caggtgcgtg atgtcttccg gctcgacggg cgctcagcgc tcgtgacggg
     1381 tgcgggcagc ggcgtcggtg aggcgatcgc cagggccttc tcaacagccg gagccgcggt
     1441 gctggtgacc gacatcgata aggacgccgc agtggctgcc gcggaatcaa tcaccgccgc
     1501 aggcggtgtc gcgcaaccat tcgtactcga cgtgcgcgat tcggcggcgg cgacggaagc
     1561 atcggcggca gctgccgagc ttggcgacgg ggccctgcac atcctcgtca acaacgccgg
     1621 agccatcgcg ccggccatgt tcgcggacct ctcggaagag aagttccgcc gcatcatcga
     1681 cattcacttg gtgggcagct tcgtctgcag ccaggcggca ctgggtcacc tgcccaccga
     1741 tggcaccgga cgcatcatca acgtcacatc cgccgcgggt ctcaccggga ccatcgggca
     1801 ggccaactat ggagccgcga aggccggcat cgtcggcctg accaagtccc ttgcccgcga
     1861 gcttgcgcgc caatccataa ccgtcaacgc cgtcgctccg ttggccgcca cggcgatgac
     1921 cgagaacatt cgcagcaacg aaaagctggc cgccaagacc ctggcacgaa tccccctggg
     1981 gcgctgggcc ttcccagacg agatcgcccc gactttcgtc ttcctggcct cagatgccgc
     2041 gtcgtacatc accggacagg tgctgcccgt cgacggcgga acggtgatct gatggcgcat
     2101 aacgtgaccg gacccttcgc ctcgaccggc cgcgaagtct atatcgccga ggccgtccgc
     2161 accccgaccg gcaagtccca tccggagcgc ggctggtatc gcgacattca cccgaacgcg
     2221 atgctggccg cctgctacac ggagctggcc gcgcgcagcg ggatcgagcc ggcgctcgtc
     2281 gaggatctag ttatcggttg caccgcgccg ttcggcgaac aatcacgcaa catcgcacgc
     2341 aacgcctggc tgcaggccgg ttatcccccc gaggtccctg ccaccacgct ggaccgtcgc
     2401 tgcggatcgg cacagaccgc agtaacaatg ggcgccgccc tggtggcctc gggcacacac
     2461 gacgtcgtga tcgccggcgg cgtcgagcac atgggccacg tccctatcga ctcgcccggc
     2521 aaggtctccg acctgtacgg cgacccgtgg ccggcggagc tgcatgaccg ctacgccttt
     2581 gtgcaccaag gcgagagcgc cgagctgatc gcggaccgct ggcagcttgg ccgcgacgag
     2641 atggatgcct tcgccgcgcg gtcacatcaa aatgccgcca aggcgatcgc cgacggccga
     2701 ttcgtcggcg agatggtccc gatgacgctc gacggccagc tccggcaggc cgaccaatgc
     2761 gtgcggccgg aaaccaccgc ggagacgctt gccaatctga agccggtgtt ccgcaaggag
     2821 aacggccgaa tcactgccgg ctcgtcgtca ccgatcagtg atggtgcggc ggcggtgctg
     2881 ctgtgctcgg gagaagccgc acaccggctc ggtctccgcg tacgtgcacg catcgtcgac
     2941 cagaccaccg tcggcgtgga cccgatcatc atgctgacag ggcccattcc ggcgacctgg
     3001 aagctgttgc agcgcaataa cctcggcatc gatgacatcg acctcttcga ggtcaatgag
     3061 gcgttctcct cggtggtact cgcgtggcaa cgtgagctgc taccggatgt tgaccgcgtc
     3121 aacgtcaacg gtggcgcgat cgcgctgggc catgccgtcg gtgccaccgg cgctaggctg
     3181 atggccacgc ttgtcgccga actcgaccgt cgcgaagccg atctcggcct tgtcacgatg
     3241 tgttgcggcg gcggtttggg caccggcacc ctcatccagc gagtaccgac gtgatcgacc
     3301 tcggcaagta cataacggcg ggcgccggcg tgtggtgggg ccaagccgcc gcggagccac
     3361 gtccactcgt ggacgcactg atcacgcaga acccgcgaat cggcccgatc cggacgttca
     3421 ccggcctgag ttggaacgac cagctcgctg tcacgatgcc cagccgggtg tcgatggtgt
     3481 cctacggcgg cctcggcgag cttcgcgagc tgagccggtc cggacggctg gaggtggtgc
     3541 cgtgccacta ctcggcgctc ccccgaatgt tcgccgaacg ccgactcccg tgcgacgtcg
     3601 gcctggtgca agtgtcggca ccggacgaaa acgggcgatg ctcgctgggt atcggtgtcg
     3661 actacgtcgc agatgccctg cggcacaccc cggtgctcat cgccgagatc aaccagagga
     3721 tgccggtcac cgttggcacg ccccggattc cgctcgatcg gttcgccgcg accatcatca
     3781 ccgaccggcc cctacccgaa gaccccaccc gtgcacccgg gctaaccgag caacgcatcg
     3841 ccgcccacgt cgccgagctc gtcgacgacg gcgacacggt gcaactgggc gtgggctcac
     3901 tgggcgcagc cgttctcgac gcgctggccg tacacgagga cttgggcttt cacaccggca
     3961 tggtgaccga cggattgctg cggctggtcg acaagggcgt ggccaccggc agccgcaagg
     4021 agatcgaccc cggcctgatt gtcgcaggca ccgcgttggg ttcggctgag ctctatcagc
     4081 gggtgcccga gctgcccgcg aagttcctgc cgaccagtta tacccatagc ccgcaagtac
     4141 tttcgcagct gcgctcgctg gtggccgtga acttcgccgt cgaggtcgac ctcagcggtc
     4201 aagtcggcgc tgaggtcagc cgcggggtct atgtcggcgc cgtcggcggt caggtcgact
     4261 tcgcgagggc cgccgcgttg accggcaagc ggtcgatcat cgcgctgcgg gccacatcgg
     4321 gtggtcggtc aacgatcaaa gtcgcactcg acggcggcgc cgtcaccact tcccggtccg
     4381 acgtcgacgc cgtggtcacc gaatacggcg tcgcgcacct gcgcggctgc agcatccgcg
     4441 agagggccga acggatgatc gcgatcgccg cgccggagtt ccgcgaccag ctcgatcagg
     4501 agaggaccaa cctgtgaccc tgcccagtca cgtcgagata cgcgaagtcg ggccgcgcg
//
LOCUS       DASRJA010000475         5150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2430869,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000475 DASRJA010000000
VERSION     DASRJA010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2430869"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFC01_20880"
     CDS             <1..495
                     /locus_tag="VFC01_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005246430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HZN82115.1"
                     /translation="HICEDEVVLERVDQNGVPVGPDEPAVRTLATGLANRTFPFIRYD
                     LGDEVTLLPDPCECGSAFARVADIGGRRDDDFRYGSITVPAITFRHVLGTDPHISEYQ
                     VTQTSTGADILTVGRPDADSLTTSVVAALRRCGLPNPTVRISVVDRIARNQASGKLKR
                     FIAL"
     gene            606..821
                     /locus_tag="VFC01_20885"
     CDS             606..821
                     /locus_tag="VFC01_20885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82116.1"
                     /translation="MVGAKRVPGSETTPPGAGGSLDGIRWMKGLLLAVIAICALAGIV
                     STASMGQTQAALAIGIIALAFFSRVGC"
     gene            867..1292
                     /locus_tag="VFC01_20890"
     CDS             867..1292
                     /locus_tag="VFC01_20890"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZN82117.1"
                     /translation="MALSKEEREQFLAEPHIAALSVSAGDKRGPLTVPIWYQYSPGGE
                     PWVITGTGSRKHRLIETQGEFTLMAERLEPTVRYVTVDGPVSRIEDGTDEHLVEMTKR
                     YLPPDKVDAYLEFARREHFPMVAIWLKPQHWLSADLGSI"
     gene            1292..1888
                     /locus_tag="VFC01_20895"
     CDS             1292..1888
                     /locus_tag="VFC01_20895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN82118.1"
                     /translation="MASRQLLFKTFYRLGFVPWDGHPLAQSLRDLVEGGGALTPGTAL
                     DLGCGTGDNSIYLAKHGWQVTGVDFVAKAVEKARAKAEANKLTVNFARADVTRLSSEG
                     VGTNFGLIVDNGCLHGMSPEDRNAYVREVTAVAAPGARLLLVEFVTGGSYGVPGIDPD
                     EVKTRFAADWALLSSGDEPALDHNGKNPARHYLFQRAS"
     gene            complement(1873..3354)
                     /locus_tag="VFC01_20900"
     CDS             complement(1873..3354)
                     /locus_tag="VFC01_20900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZN82119.1"
                     /translation="MPESFTETFAAGLASYGAQECIEFEGRWYSGREVAAYGVAIEAA
                     LHEAGVSSGEPVGLVVRNRLPHAAAIIGFLAAGRPVSMIYSFQSPEAIGRDIEKLNLS
                     AIVAETQDWTDPVIAAAKHGGSAGVAISLTPPRVTTVAGLEHRDSSRHHADVESGVAL
                     QILTSGTTGPPKRQDIKTSVLERTVFSVTSGEKAPPGAPPEFAYWQFGGIGVCQLVAG
                     VYNARRIVMLERFTVDGFVNAIKTHGVKRSGVQPAVIRMLLDADVPKEHLASLEFLIS
                     ASGPLDPETRDEFEKHFDIPVRLAYGATEFAGSLCAWTPDDIDKFGDSKRNSVGRPLP
                     DTQVRIVDQATGTEMPVGEQGLLEARVAPISADWIRTTDIASVDADGFVTLHGRADGA
                     INRGGFKILPETVRRVLISHPAVRDACVVGVPDQRLGQVPFAAIEVNRGVVLPSDDEL
                     KELVRQSLPVYNVPVAFVVVDELPRNPALKVSLPEVAALYEAR"
     gene            complement(3458..3748)
                     /locus_tag="VFC01_20905"
     CDS             complement(3458..3748)
                     /locus_tag="VFC01_20905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82120.1"
                     /translation="MKRCLVGGLTALLMAAELIASAPSATAGCIYGGPFLSKCDGPVQ
                     PDGTWQRCVAAPQLIPRGASSYLVPVKRCELMSPDLRPWDLAVADPPTHIND"
     gene            complement(4175..>5150)
                     /locus_tag="VFC01_20910"
     CDS             complement(4175..>5150)
                     /locus_tag="VFC01_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020109556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN82121.1"
                     /translation="SAAARLRQFAMFERMCASPGMARASLEASFRLDLRPILPTITAP
                     TLVIHAREDPTVPVQGGRYLADHISGARYLEVEGVDHPPYLTEPDKVLTGIEEFLTGS
                     HAAPAQSRRALRSVLFTDIVASTQHAAETGDERWRAVLHRFGEITTELAQRFGGTVVK
                     STGDGHLMTFDGPTQAIRCAEAFRADAETLGIEIRAGIHTGECELMDADIGGIAVHIA
                     ARILGRAGAGEILVSSTVRDLVVGSGTGFEDRGSVELRGVPGTWQLLAVDRHGARAGS
                     AEAQLVSTPTPGPRIAMRRSDRAVAAMARRTPWILRGVARLAPATARE"
BASE COUNT          878 a         1631 c         1750 g          891 t
ORIGIN      
        1 cacatctgcg aggacgaggt ggtgctggaa cgcgtggacc agaacggcgt gccggtgggt
       61 cccgatgagc ccgcagtgcg gacgctcgcc accgggctgg ccaatcggac gttcccattc
      121 atccgttatg acctcggcga cgaggtgacg ttgctaccgg atccgtgcga gtgcgggagc
      181 gccttcgcgc gtgtggcaga catcggcggg cgacgcgacg acgatttccg ctatggctca
      241 attacggtgc ccgccatcac tttccgtcat gtactcggca ccgacccaca catctcggaa
      301 taccaagtca cgcagacctc cacgggcgcc gacattctca cggtcggacg tcctgacgcc
      361 gactcgctca cgacatcggt ggtcgccgcg ttgcgccgct gcggcctgcc gaacccgacg
      421 gtaaggatca gcgtcgtcga ccgcatcgcg cgtaaccaag cgagcggcaa gctcaagcgc
      481 ttcatcgcct tgtagacaat cgggattaaa aagacatcac gaattaaccg accagcgctg
      541 atcatgagat atggtgtggc tcacaacggc gacaacgtgt gagctggagc acagaggagg
      601 cgcccgtggt cggcgcaaag cgtgtgcctg ggtccgagac gacgcctccc ggtgccggcg
      661 ggtcgctcga cggcatccgg tggatgaagg gcttgctcct ggcggtcatc gcgatatgcg
      721 cgctcgccgg catcgtgagc accgcttcga tggggcagac gcaggcggcg ctggccatcg
      781 gcatcatcgc gctggcgttc ttctcccggg tcgggtgctg atcgtcgatc ggtcgttttg
      841 acggaggccg cggctagcct ggcggaatgg ccctctccaa agaagaacgc gaacaatttc
      901 tggccgagcc gcatatcgcg gcgctatcgg tgagcgcagg tgacaagcgc ggaccactga
      961 cggtgccgat ctggtaccag tacagccccg gcggcgaacc gtgggtgatc accggcaccg
     1021 gctcacgcaa gcaccgcctc atcgagacgc agggtgaatt cacgctgatg gctgagcggc
     1081 tcgagccgac ggtgcgctat gtcacggtcg acggtccggt gagccgcatc gaggacggca
     1141 ccgacgagca tctcgtggaa atgaccaagc gctacctccc gccggacaag gtcgacgcct
     1201 atctggagtt cgcccgtcgc gagcacttcc cgatggtggc catctggttg aagccccagc
     1261 actggttgtc tgccgacctg ggctctatct gatggcgtcg cgccaacttc tgttcaagac
     1321 cttctatcga ctgggcttcg tgccatggga tggccatccc ctggcgcaga gcctgcgcga
     1381 cctggtcgaa ggcggcgggg cgttgacgcc cggcaccgcg ttggatctcg gctgcggcac
     1441 cggtgataac tccatttacc tggctaaaca tggctggcag gtgacaggtg tggacttcgt
     1501 cgcgaaggcg gtcgagaagg cccgcgccaa ggccgaggcc aacaagctga cagtgaactt
     1561 cgcccgagcg gacgtgacac ggctgtcctc ggagggcgtc gggacgaact tcgggctgat
     1621 cgtcgacaac ggctgcctgc acggcatgag ccccgaggat cgcaacgcct acgtgcgtga
     1681 ggtcaccgcg gtcgcggcgc ctggcgcgcg gctgctgctg gtcgagttcg tcaccggcgg
     1741 ctcgtatggc gtaccgggca tcgatcccga cgaagtgaag acgcgcttcg ccgcggactg
     1801 ggcgctgctg tcatcgggtg acgaaccggc gttggatcac aacggcaaga acccggcgcg
     1861 ccactacctt tttcagcgag cttcgtagag cgcggcgacc tcgggcagac tcaccttcag
     1921 cgcggggttg cgcggaagtt cgtcgacgac gacgaaggcg accggcacgt tgtagaccgg
     1981 cagtgattgg cgcacaagtt ctttgagttc gtcgtcggag ggaaggacca cacctcggtt
     2041 tacctcgatc gccgcgaacg gcacttggcc gaggcgctgg tcgggcacgc ccaccacgca
     2101 ggcgtcgcgc acggcaggat gtgaaatgag aacgcgccga acagtttccg gcaggatctt
     2161 gaagccaccg cggttgatcg cgccgtcggc tctgccgtgc agagtgacga acccgtcggc
     2221 gtcgaccgag gcgatgtcgg tggtacggat ccagtcggcg ctgatcggtg ccaccctggc
     2281 ctcgagcaga ccctgctcgc cgacgggcat ctcggttcca gtggcctggt cgacgatgcg
     2341 cacctgagtg tcgggcaagg gtcggcccac gctgttgcgt ttggaatcgc cgaacttgtc
     2401 gatgtcgtcg ggcgtccagg cgcacagtga ccctgcgaat tcggttgcgc cgtaggccaa
     2461 ccggactggg atgtcgaaat gcttctcgaa ttcgtcgcgc gtctccggat cgagcggacc
     2521 cgaagcgctg atcaagaatt ccagcgacgc caggtgttcc ttgggcacgt cggcgtcgag
     2581 cagcatgcgg atgacggcgg gctgcacgcc ggaacgcttg acgccgtggg tctttatcgc
     2641 gttgacgaac ccgtcgacgg tgaaccgctc gagcatgacg atgcgccgcg cgttgtaaac
     2701 gccggcgacc agctggcata ccccgatacc gccgaactgc cagtaggcga actccggcgg
     2761 ggcgccgggc ggggccttct ctccgctggt cacgctgaac acagtgcgct ccagcaccga
     2821 cgtcttgatg tcctgtcgct tcggcgggcc ggtggtgccg ctggtcagaa tctgcagggc
     2881 cacaccggat tcgacgtcgg cgtggtggcg gctgctgtcc cgatgttcca gtccggcgac
     2941 ggtcgtgacg cgcggtgggg tcagcgagat cgccacgccg gcgctgccgc catgcttcgc
     3001 ggccgcgatg acggggtccg tccagtcctg tgtctcggcg acaatggccg acaggttcag
     3061 cttttcgatg tcgcggccga tcgcttcggg cgactggaag gagtagatca tcgaaaccgg
     3121 gcggccggcg gcgagaaagc cgatgatcgc cgcggcatgc ggcagccggt tgcggaccac
     3181 gagaccgact ggctcgccgg acgagacgcc ggcttcgtga agcgctgcct cgatcgccac
     3241 gccgtatgcg gccacctcac gaccggagta ccaccggccc tcgaactcga tgcattcttg
     3301 agcgccatag ctagccagcc ctgccgcgaa ggtttcggta aagctttcgg gcacgactag
     3361 acgatagcca aagtggccgc atcacgaaga ttgcacagag ggtcgtggcg cgccgcaatc
     3421 cacagccctg actgcaatct tcagctcccc ttgcgactca gtcgttgatg tgcgtcggcg
     3481 ggtcggcgac agcgagatcc caagggcgca ggtcaggact catcaattcg cagcgcttga
     3541 cgggcacgag gtaggagctg gcgccgcgag gaatcaattg gggcgctgcc acgcaacgtt
     3601 gccaggtgcc gtcaggctgg acgggtccat cgcacttgct gagaaagggt cctccgtata
     3661 tgcagccagc ggtggccgac ggcgctgagg cgatcagctc tgcggccatc agcagggcgg
     3721 tcaatcctcc gacgaggcag cgcttcatgg ttgtctcccg tcacatcgcc gctggggcgt
     3781 tctcgggcgc ccgggggtgg caaataccgt cgacagccca gccagcaacc atgacgctac
     3841 cgcttatgta attcgttttt gctgactttg cggtcgtgat ccgaagcatt agaacgaata
     3901 tgaaccaccg aagcgaaccc acgatttctt cgacaccgtt caatgcccga cattggatcg
     3961 cagcggcgag accatcggcc gcaacgacag cggtggagac gtggcggact gggttgggtg
     4021 aaggccgctg agcaggtggt ggtaccgact ttgttaagga cctttagctt ccgagaccga
     4081 ggtcagtctg ggcctcccgg ctcttacctt gcaacggtcg ctattcctca cggtgtgagg
     4141 gcgtaaacca cgttgcggct caacgtatct ccgtttattc gcgggcggtg gccggggcga
     4201 ggcgggccac cccgcgcagg atccacggcg tgcgcctggc catcgcggcc acggcgcggt
     4261 ccgagcggcg catcgcgatg cgcgggccgg gggtcggcgt tgataccagt tgcgcctcgg
     4321 ccgatcccgc ccgcgcgccg tggcgatcga ccgccaggag ttgccaggtg cctggcacgc
     4381 cacgcagctc gacgctgccg cggtcctcga agcccgtgcc cgagccgacg accaggtccc
     4441 gcacggtgct cgagacgagg atttcaccgg cgccggcacg gccgaggatg cgcgccgcga
     4501 tgtgtacggc gatcccgccg atgtcggcgt ccatcagttc gcactcgccg gtgtggatgc
     4561 cggcgcggat ctcgatgccc agcgtttctg catctgcgcg gaacgcctcg gcgcagcgga
     4621 tggcctgcgt cgggccgtcg aacgtcatga ggtggccgtc gccggtgctc ttgaccaccg
     4681 tgccgccgaa tcgttgcgcg agttcggtgg tgatctcacc gaatcggtgc agcaccgcgc
     4741 gccaccgttc gtcgcccgtc tccgcggcgt gttgggtcga cgcgacgatg tcggtgaata
     4801 gcacggagcg cagggcgcga cgcgactgcg ccggcgcagc atggctgccg gtgaggaact
     4861 cctcgatccc ggtcaagact ttgtcgggct cggtcaaata gggcgggtgg tccaccccct
     4921 cgacctccag gtaccgcgcg ccggaaatgt ggtcggcgag ataacggccg ccctgcaccg
     4981 gaaccgtagg gtcctcgcgg gcgtggatga ccagggttgg cgcggtgatc gtcggcagga
     5041 tcggccgcag gtcgaggcga aacgacgctt cgagtgacgc gcgtgccatg cccgggctgg
     5101 cgcacatgcg ttcaaacatg gcgaactggc gtaatcgagc ggccgctgat
//
LOCUS       DASRJA010000476         4509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1707871,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000476 DASRJA010000000
VERSION     DASRJA010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707871"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFC01_20915"
     CDS             <1..788
                     /locus_tag="VFC01_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN82122.1"
                     /translation="FTFTGSSAVGKEIGKLAAEKLKPCTLELGGKSAAIILEDADLDS
                     TLPMLVFSGLMNCGQACVGQTRILAPRSRYDEVVEKVSAAVAAMQVGLPDDPASMIGP
                     LISEKQRERVEGYIKKGVEEGARIVTGGGRPEGLDGGWFVQPTVFADVTNSMTIAQEE
                     IFGPVLAIIPFDTEEDAIRIANDSVYGLAGSVFTTDFPKAVEIAKKIRTGTYAVNMYA
                     FDPGAPFGGYKNSGIGRENGPEGIEQYCEAKSILLPFGYTPEV"
     gene            893..1123
                     /locus_tag="VFC01_20920"
     CDS             893..1123
                     /locus_tag="VFC01_20920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82123.1"
                     /translation="MTRHTARKALVVAAAVAGLMMGGAGTAAADHGDDDFGFDDHGRF
                     DDDVFFFDRGGFNCFGPFCFVVDDRHGGPGRH"
     gene            complement(1120..1785)
                     /locus_tag="VFC01_20925"
     CDS             complement(1120..1785)
                     /locus_tag="VFC01_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN82124.1"
                     /translation="MTHILIAEDEQRIASFIEKGLSANGFAVTVVADGRSAYNYAATG
                     DFDLMILDIGLPELDGFEVLARLRAEGHRVPVLVLTARSSVHDTVAGLEGGADDYMAK
                     PFRFEELLARVRLRLTPTHTAGDFVLSCGGLQLDLHTRRALVGGRTVDLSAREFALAE
                     TFLRHAGQVLSREQLLSQVWGYDYDPGSNVVDVYVRYLRRKLGSERFVTLRGMGYRLD
                     AAP"
     gene            complement(1782..3344)
                     /locus_tag="VFC01_20930"
     CDS             complement(1782..3344)
                     /locus_tag="VFC01_20930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN82125.1"
                     /translation="MGATIEVETTEAAMMGATVAETAAVATAVRAEPTTGATGRRLAD
                     RLRPSAARTRIIGWVLLLVFTALGLVTFVTWRLLIAATDARMQQELRFEMEQFAELTA
                     PGVNPRTGMPFASVDEVIREAIAYNVARPNEKFLGFVDGQYRVQSRPQVDGRATLAGD
                     VDFSQRVGSIAEPARGTYRHPQLGEIVYAAVPVTLTGDPARGVIVAAYLADSERGDAD
                     DAARLMLIVGAVTLLGAVGAAWLVAGRILRPLRDITSTARTITETDLSQRIPRRGGDG
                     DELGDLVATVNGMLDRIENGISAQRRFVDDAGHELRTPITIIRGHLDVLDPTDPADVS
                     STVGLVNDELDRMNRMVSDLLLLAQSEQPAFLHPEAVDVAALTTDTFDKIALLGDRDF
                     AVEAVADIFAVLDPQRITQALVALADNACRYTDSGDRIALGSRVEGGWLRFWITDSGP
                     GVSDEDRPRIFQRFARGGAGSRRSDGAGLGLAIVDAIAVAHGGVVLLDSTPGCGATFS
                     VVVPLVPGSSER"
     gene            3398..3673
                     /locus_tag="VFC01_20935"
     CDS             3398..3673
                     /locus_tag="VFC01_20935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82126.1"
                     /translation="MLIEVTVVGANVRVAGSGGGVAAGGGGVAGGVLLGASTVIGCSR
                     LSSYSGRMLMPTIPMTPPTISAPSRIVKGSRMPSSVLTCDGSPVRPT"
     gene            complement(3738..>4509)
                     /locus_tag="VFC01_20940"
     CDS             complement(3738..>4509)
                     /locus_tag="VFC01_20940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN82127.1"
                     /translation="LTQKAYAEGLRALYLYTAAHQDVVVADIVSGADAEMADRVNDLL
                     LPIVKGVGSERAYQCLTESLQTLGGSGFLQDYPIEQYIRDAKIDSLYEGTTAIQAQDF
                     FFRKIARDQGRALQHVVGQIQAFLGSDSARPELANARTLLATALADLQAMAAAMTGYQ
                     LSSQENARELYLLGLESVRFLLAVGDVLIGWLLLQHAETALDALDSEVSDRDRHFYAG
                     KVAVAKLFAKNVLPRLTAERKIVEAVDLTVMDLREDAF"
BASE COUNT          802 a         1437 c         1488 g          782 t
ORIGIN      
        1 agttcacgtt caccggcagc tccgcggtgg gcaaggagat cggcaagctg gccgccgaga
       61 aactcaagcc gtgcacgctt gaactgggcg gcaagtccgc ggccatcatc ctcgaagacg
      121 ccgacctgga ctccacgctg ccgatgctgg tgttctccgg cctgatgaac tgcgggcagg
      181 cctgcgtggg ccagacccgc atcctggcgc cgcggtcgcg gtacgacgag gtcgtcgaga
      241 aggtatcggc ggcagtcgcc gcgatgcagg tcgggctgcc ggacgatccc gcgtcgatga
      301 tcggcccgct gatctcggag aagcagcggg agcgcgtcga gggttacatc aagaagggcg
      361 tcgaggaggg ggcccggatc gtcaccggcg gcggccggcc cgaggggctc gacggcggct
      421 ggttcgtgca acccacggtg ttcgccgacg tgaccaactc gatgaccatc gcccaggagg
      481 agatcttcgg cccggtgctg gcgatcatcc ccttcgacac tgaagaggac gcgattcgca
      541 tcgccaacga ctcggtctac ggcttggcgg gcagcgtgtt caccaccgac ttccccaagg
      601 ccgtcgagat cgccaagaag atccgcaccg gcacctacgc ggtcaacatg tacgcgttcg
      661 atcccggtgc gccattcggc ggctacaaga attcgggcat cggacgcgag aacggtccgg
      721 agggcatcga acagtactgc gaggccaaga gcatcctgct gccgttcggt tacacgccgg
      781 aggtctagcc gcgcgcgtgg atgagaacgc tctcatgcac ggttcccgtc gcactcattt
      841 cgccacacca aactgagggt gtcagcgaac caagagcgtt gggagtacag cgatgacacg
      901 ccataccgca cggaaggccc tcgtggtggc ggccgccgtc gccggtctca tgatgggcgg
      961 agcgggaacc gccgccgcgg accatggcga cgacgatttc ggtttcgatg atcacggccg
     1021 tttcgacgac gatgtgttct tcttcgaccg gggcgggttc aactgctttg gcccgttctg
     1081 cttcgttgtc gacgacaggc acggcggccc cggtcgccac taaggcgcgg cgtccaatcg
     1141 gtagcccatg ccgcgaagtg tgacgaagcg ctccgatccc agcttgcggc gcaggtagcg
     1201 cacgtacacg tcgacgacgt tggagccggg gtcgtagtcg taaccccaga cctggcttaa
     1261 cagctgctca cgtgagagca cctggcctgc atgccgaaga aacgtttcgg cgagggcgaa
     1321 ctcgcgagcc gagaggtcaa ccgtacggcc accgaccaat gcgcggcgag tgtgcaggtc
     1381 gagttgtaaa ccaccgcatg acaatacaaa gtcaccggcg gtgtgcgtcg gtgtgagacg
     1441 cagccgcaca cgagccaaca gttcttcgaa tcgaaacggc ttggccatgt agtcgtcagc
     1501 gccgccctcc aggccggcga ccgtgtcatg cacgctgctg cgcgcggtga gcacgaggac
     1561 gggaacccga tggccttctg ctcgcagccg ggcaagcacc tcgaagccgt ccaattcggg
     1621 cagcccgatg tcgaggatca tcaggtcgaa atcgccggtc gccgcgtagt tgtaggccga
     1681 ccgaccatcc gcgaccacag tcaccgcaaa gccgttggcg gacagcccct tttcgatgaa
     1741 cgaggcgatt cgctgctcgt cctcggcgat caagatgtgg gtcatcgctc gctcgagcca
     1801 gggacgagag gcacgaccac actgaaggtg gcgccgcacc ctggtgtgct gtcgagtaga
     1861 accacaccgc catgggcgac ggcgatggcg tcgacgatcg ccaagcccag tccggcgcca
     1921 tcggaacgtc gactgccggc gccacctcgg gcgaagcgct ggaagatcct gggccggtct
     1981 tcgtcgctga cgccggggcc ggagtcggtg atccagaaac gcagccaccc gccttcgacg
     2041 cgtgagccga gggcgatccg gtcgccggaa tcggtgtacc tgcaggcatt gtcggcgagg
     2101 gcaaccagcg cttgggtgat gcgctggggg tcgaggactg cgaatatgtc ggccaccgcc
     2161 tccacagcga agtcacggtc gccgagcaga gcgatcttgt cgaacgtatc ggtcgtcagt
     2221 gctgcgacgt cgacggcttc gggatgtaag aaggccggtt gttcggactg agccagcaag
     2281 agcaggtcgg aaaccatgcg gttcatccga tccagttcgt cgttcaccag gcccaccgtc
     2341 gagctgacgt cggctgggtc ggtgggatcg agcacatcca ggtgcccacg gatgatcgtg
     2401 attggcgtgc gcaattcgtg gccggcgtcg tcgacgaatc gacgctgagc tgaaataccg
     2461 ttctcgatcc gatcgagcat gccgttgacc gtggccacca ggtcaccgag ttcgtcgcca
     2521 tctccgccac ggcgcgggat ccgttgcgac aggtcggttt cggtgatggt ccgtgctgtg
     2581 ctggtgatat cccgcagcgg acgcaagatc cgtcccgcca ccagccacgc ggcaccgacc
     2641 gcgcccaaca aagtcaccgc accaacgatc agcatgagcc gggcggcgtc gtctgcgtca
     2701 ccgcgttcgc tatcggccag gtaggccgcg acgatcactc cgcgtgccgg atcccccgtc
     2761 agcgtcaccg gtacagcggc gtaaacgatt tcgccgagct gaggatgtcg atacgtgccg
     2821 cgagcgggct cggcgatcga ccccacccgc tgggagaagt cgacgtcacc ggccaatgtg
     2881 gcacggccgt cgacctgagg cctgctctgc acgcggtact ggccgtcgac gaagccgagg
     2941 aatttctcgt tgggcctggc aacgttgtat gcgatcgcct cccgaatcac ctcgtcgacg
     3001 cttgcgaatg gcattccggt tctcgggttg acaccgggcg cggtcagctc ggcgaactgc
     3061 tccatctcga agcgcaactc ctgctgcatg cgggcgtctg tagcggcgat gagcaacctc
     3121 catgtcacga aggtgaccag accgagcgcc gtgaagacca gtaacaggac ccatccgatg
     3181 attcgcgtgc gcgccgctga tggacgcagg cggtcagcca gtcggcgccc ggtggcgccc
     3241 gttgttggct cagcccggac cgctgttgct accgctgcgg tctccgccac cgtggccccc
     3301 atcatcgccg cctctgtcgt ctccacctct atggtggccc ccatcgtcgg tcgtctcgat
     3361 cggatcgaag gttgccggtg gcggtacggg gacgtcggtg ctaatcgaag tcacggtggt
     3421 cggcgccaat gttcgggtcg ccggaagtgg cggcggtgtc gccgcaggag gcggcggggt
     3481 ggcgggcggc gtgctgctcg gtgcctcgac ggtgatcggc tgtagccggc tgtcgtcata
     3541 ttccggccgc atgttgatgc cgacgattcc gatgacgccg ccgacgatca gcgcgccgag
     3601 cagaatcgtc aagggaagcc gcatgccatc gagcgtcctc acctgcgatg gctcccctgt
     3661 gagacccaca tgagaacatt ctcatcaaac ggacggccgc ggttacccgc ctgacagttg
     3721 aatcgccaca tcgtcgtcta gaacgcgtct tcgcgcaggt ccatcacggt gaggtcgacg
     3781 gcctcgacga tcttgcgttc ggcggtcagc cgtggcaata cgttcttcgc gaacaatttt
     3841 gccacggcga cctttccggc gtagaagtgg cggtcgcggt cggagacctc gctgtcgagc
     3901 gcgtcgagcg cggtctctgc gtgctgcagc agtagccacc cgatcaacac gtcgccgacc
     3961 gccagcagga accgaaccga ctccagccca aggagataca gttcgcgggc gttctcctgc
     4021 gaggacagtt gatagccggt catggctgct gccatcgcct gcaggtcggc cagcgcagtg
     4081 gcgagaagcg ttcgtgcatt ggctaattcg ggcctcgcgc tgtcgctgcc gagaaacgcc
     4141 tggatctgac cgaccacgtg ttgcagggcc ctgccttggt cgcgggcgat cttccggaag
     4201 aagaagtcct gcgcctggat cgctgtggtg ccttcgtaga gcgagtcgat cttcgcgtca
     4261 cgaatgtact gctcgatcgg atagtcctgc aggaagccgg aaccgcccag cgtttgcagt
     4321 gattcggtca ggcactggta ggcgcgttcg gagccgacac ccttcacgat cggcagcagc
     4381 agatcgttga cccggtcggc catttcggcg tcagcgccgg acacgatgtc ggcgacgaca
     4441 acgtcctgat gggcagccgt gtagaggtac agggcccgca gcccctcggc atatgccttc
     4501 tgcgtgagc
//
LOCUS       DASRJA010000477         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_72928,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000477 DASRJA010000000
VERSION     DASRJA010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72928"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VFC01_20945"
     CDS             <1..1037
                     /locus_tag="VFC01_20945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN82128.1"
                     /translation="WNEAGKLGFIGVNLPEEYGGGGAGMYELSLVMEEMAAAGSALLM
                     MVVSPAINGTIISKFGTEDQKKRWLPGIADGSTTMAFAITEPDAGSNSHRITTTARRD
                     GSGWILKGQKVFISGIDQAQAVLIVGRTEELKTGNLRPALFIVPTDTKGLSWTKIEME
                     LVSPESQFQVFLDEVRLPSDALVGSEDAAIAQLFAGLNPERIMGAASAVGMGRFAINK
                     AVDYVKTRQVWKTPIGAHQGLSHPLAQNHIEVELAKLMMQKAATLYDMGDDAGAAEAA
                     NMAKYAAGEASVRAVDQAVQSLGGNGLTKEYGIAAAVTASKLARIAPVSREMVLNFVA
                     QTSLGLPRSY"
     gene            1037..1804
                     /locus_tag="VFC01_20950"
     CDS             1037..1804
                     /locus_tag="VFC01_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HZN82129.1"
                     /translation="MATLVHYTVEGSVARLTLDSPDNRNALSTALVNQLHQGLTDATE
                     ESGVRAVVIDHTGGTFCAGADLAEAAGRDRGDVAVDRARELTHLLRRILKLPMPVIAA
                     VDGHVRAGGLGLIGACDIAVAGPTSTFALTEARIGVAPSIISLTLLPKMTARSASRYF
                     LTGEKFGAVEAAEIGLITIAADDVGASVAELTAAIGKASPQGLAASKALTTAAILEAF
                     DRLADELTEQSAALFVSDEAREGMLAFLQKRPPHWVR"
     gene            1804..2565
                     /locus_tag="VFC01_20955"
     CDS             1804..2565
                     /locus_tag="VFC01_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZN82130.1"
                     /translation="MVREVLITGATGTLGRKLVGAATAAGHHVRAMSRRSHVGYTGVH
                     WSQGDLLANTGVDAAVEGVDVVVHCATQGTGDKDVTSTENLITAARRAGVAHIVYVSI
                     VGIDRIPLPYYKTKLRVERDLEASSVGHTVLRATQFHDLIKMSFSIQRFSPLLWTLKG
                     VRFQPIDTRDVTSRLVELIDCEPAGRVADIGGPAVQTHAELARIYLAARGGRRPVFEL
                     PVPGRIVAGYRAGANLAPDNAVGTVGFEEYLARTE"
     gene            2660..>2921
                     /locus_tag="VFC01_20960"
     CDS             2660..>2921
                     /locus_tag="VFC01_20960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HZN82131.1"
                     /translation="MSTPASRNGSMRAADTDRIQVAQLLTDAAAQGQLRMSEYEDRLT
                     RAYAAQTYDELDRLAADLPGAVTRGRSGGPCRPAPSTLLLAIM"
BASE COUNT          497 a          958 c          992 g          474 t
ORIGIN      
        1 tgtggaacga ggcaggcaaa ctcggcttca tcggggtcaa cctgcctgag gagtacggtg
       61 gcggcggcgc gggcatgtac gagctgtctc tggtgatgga ggagatggcc gctgcgggct
      121 ccgcgctgct gatgatggtg gtgtcccccg cgatcaacgg cacgatcatc tccaagttcg
      181 gcaccgagga ccagaagaag cgctggctgc ccggcatcgc cgacggctcg accacgatgg
      241 cgttcgcgat caccgaaccc gacgccggat cgaacagcca tcgcatcacc acgaccgccc
      301 gccgcgacgg cagcggctgg atcctcaagg gccagaaggt gttcatctcc ggcatcgacc
      361 aggcgcaggc cgtattgatc gtcgggcgca ccgaggaact gaagaccggc aacctgcggc
      421 cggcgctgtt catcgtcccg accgacacca agggcctttc gtggaccaag atcgagatgg
      481 aactggtcag ccccgagagt cagttccagg tgttcctcga cgaggtgcgg ttgccgtccg
      541 acgcgctggt cgggtcggag gacgccgcga tcgcgcagct gttcgcagga ctgaatccag
      601 agcggatcat gggcgcggcc agcgcggtgg gcatgggccg gttcgcgatc aacaaggccg
      661 tcgactacgt caagacgcgc caggtgtgga agacgccgat cggtgcgcat cagggactat
      721 cgcatccgtt ggcgcagaac cacattgagg tcgagctggc taagctgatg atgcagaagg
      781 cggccacgct ctacgacatg ggcgacgacg ccggtgccgc cgaggcagcg aacatggcca
      841 agtacgccgc gggtgaggcg tcggtgcgcg ccgtcgacca ggcggtgcag tcgctgggtg
      901 gcaacgggct caccaaggag tacggcattg ccgccgccgt gacggcatcg aagctggcgc
      961 gcatcgcgcc ggtcagccgt gagatggttc tcaactttgt ggcgcagacc tcgctcggtc
     1021 tgccacgctc atactgatgg ccacactcgt ccactacacc gtcgagggca gcgtcgcgcg
     1081 gctgacgctc gattcgccgg ataaccgcaa cgcgctgtcc actgcactgg tcaaccagct
     1141 gcaccagggc ctcaccgacg ccaccgagga gtccggtgta cgggccgtgg tgatcgacca
     1201 caccggcgga accttctgtg cgggcgcgga tcttgccgag gcagcggggc gtgaccgcgg
     1261 agacgtggcc gtcgaccgag cccgcgagct gacccacctg ctgcggcgga tcctcaagct
     1321 gccgatgcca gtgatcgccg cagtcgacgg gcatgtgcgt gcaggcgggc tcggcctaat
     1381 cggcgcatgc gacatcgcgg tcgccggacc gaccagcacg ttcgcattga ccgaggcgcg
     1441 catcggcgtt gcgccatcga tcatttcgct gaccctgctg ccgaagatga ccgcccgctc
     1501 cgcgagccgc tatttcctca ccggcgagaa gttcggcgcg gtcgaggccg ctgagatcgg
     1561 tttgatcacg atcgccgccg acgacgtcgg ggccagcgtt gcagagctga cggccgcgat
     1621 cggcaaggcg tcgccgcagg gcctggccgc ctccaaagcg ttgaccaccg ctgccattct
     1681 ggaggcgttc gaccggctcg ccgacgagct gaccgagcag tccgcggccc tgttcgtctc
     1741 cgacgaggcg cgcgagggca tgctggcatt cctgcagaag cgcccgccgc actgggtgcg
     1801 ctgatggttc gcgaagttct catcacaggc gcgaccggca ccctcggccg gaagctggtg
     1861 ggcgcggcga cggctgccgg tcatcacgtg cgcgccatga gtcggcgcag tcacgtcggc
     1921 tacacgggtg tgcactggtc ccagggtgac ctgctcgcca acaccggcgt cgacgctgcc
     1981 gtcgaaggcg tggacgtggt cgtgcactgc gccactcaag gcaccggcga caaggacgtc
     2041 acctcgaccg agaacctcat caccgcggcg cgcagagccg gcgtcgctca tatcgtctat
     2101 gtgtcgatcg tcggcatcga ccgaattccg ctgccctact acaagacaaa actgcgcgtc
     2161 gagcgcgacc tggaagcatc gagcgtcggg catacggtgt tgcgcgccac gcagttccac
     2221 gacctgatca agatgagctt ctcgatccag cggttctccc cgctgctgtg gacgctgaaa
     2281 ggtgtgcggt tccagcccat cgacacacga gatgtcacct cacggctggt cgagctgatc
     2341 gactgcgaac cggcagggcg ggtcgccgac atcggcggcc cagccgtgca gacacatgct
     2401 gagctggcac gcatctacct cgcggcccgc ggcggccgtc gacccgtctt cgagctgccg
     2461 gttccggggc gcatcgtcgc gggttaccgg gcgggcgcaa accttgctcc ggacaacgct
     2521 gtgggcaccg tcggcttcga ggagtacctg gcgcggacgg aataaagttt gctcatctat
     2581 ctgcttcgcc gtggctggcg agggcacagt ttaggcaacc gccttgcatg tgccagcgtt
     2641 cgtcgtaggc tcgacgttga tgagcactcc agcgtcgcgg aacggttcga tgcgtgctgc
     2701 tgacaccgat cggattcagg tggcgcagtt gctcactgat gccgctgccc agggccagct
     2761 gcggatgagt gagtacgagg atcgtctgac cagggcgtat gctgcccaga cgtacgacga
     2821 actcgaccgg ctggccgccg atctgcccgg cgccgtcacc cggggccgca gcggtggccc
     2881 gtgccggccc gcgccgtcga ctctgctgct ggcgatcatg a
//
LOCUS       DASRJA010000478         6191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1561353,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000478 DASRJA010000000
VERSION     DASRJA010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561353"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /gene="murD"
                     /locus_tag="VFC01_20965"
     CDS             complement(<1..231)
                     /gene="murD"
                     /locus_tag="VFC01_20965"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893864.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZN82132.1"
                     /translation="MDLSGAKVLVAGARVTGRAILAALTPLGARATLTDDSPAALTEF
                     AQHGVAVLDAANAADRITDYDLVVTSPGLPPTA"
     gene            complement(233..1312)
                     /gene="mraY"
                     /locus_tag="VFC01_20970"
     CDS             complement(233..1312)
                     /gene="mraY"
                     /locus_tag="VFC01_20970"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZN82133.1"
                     /translation="MRQILIAVGIALTISILLTPVLIRLFTRQGFGHEIREDGPPSHH
                     KKRGTPSMGGVAIVAGIWAGYLGTQLIGIVMDGKGPSASGWLVLGLATALGAVGFVDD
                     LIKIRRSRNLGLNKTAKTVGILTAAVLFGVLALQFRNADGLTPGSAELSYVREIATVT
                     LAPAVFVLFCIVIVSAWSNAVNFTDGLDGLAAGAMAMVCAAYVLITFWQFRNACATSP
                     GLGCYNVRDPLDLAIIAAATAGACIGFLWWNAAPAKIFMGDTGSLALGGIIAGLSVTS
                     RTEMLAVVLGALFVAEVTSVVVQIMAFRTTGRRVFRMAPFHHHFELVGWAETTVIIRF
                     WLLTAIACGLGVALFYSEWLTTVGA"
     gene            complement(1309..2796)
                     /gene="murF"
                     /locus_tag="VFC01_20975"
     CDS             complement(1309..2796)
                     /gene="murF"
                     /locus_tag="VFC01_20975"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZN82134.1"
                     /translation="MIDLTVAQIADIVGGTLTDISSDDAAATRVTGTVEFDSRAVTPG
                     GLFLALPGARSDGHDFAAAAVGAGAVAVLAARPVGVPAIVVAPELAHDPAAGVLEHDT
                     DGSGAAVLAALARLASAVAAELVDGGLTIVGITGSSGKTSTKDLLAAVLGPLGEVVAP
                     PGSFNNELGHPWTVLRATTETDYLVLEMSARHPGNIAALASIAPPSIAVVLNVGTAHL
                     GEFGSREAIAATKAELPQAVPSSGVVILNVDDTAVAAMADQTAARVVRVGRTPDADVW
                     AGPVTLDALARARFTLHTADGQTDVALAVHGDHQVSNALCSAAVALECGASLQQVATA
                     LAAAGPASKHRMQVTTRDDGVTVINDAYNANPDSMRAGLKALAAIAHEGGAKRRSWAV
                     LGEMAELGDDAISEHDRIGRLAVRLDVSRLIVVGTGRSMSAMHHGAVMEGSWGPESTM
                     VDDANAALALLRAELHAGDVVLVKASNAAGLGALADALADEDVRG"
     gene            complement(2793..4307)
                     /locus_tag="VFC01_20980"
     CDS             complement(2793..4307)
                     /locus_tag="VFC01_20980"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZN82135.1"
                     /translation="MNLRPNDPVGQALGRLAEQVGAMPAGSSAVPNVHVIGVTLRGQD
                     ARPGDLFAALPGSSSHGGRYAADAVAHGAVAVLTDPAGVDAMGGDVGVPVLIHPDPRS
                     VLGELAAAVYGHPSDRLRVIGVTGTSGKTTTTYLVEAGLRSAERVAGLIGTVGVRIDG
                     RDQPSALTTPEAPDLQALLAVMVEQGVDTVVMEVSSHALTLGRVDGVHFEVGGFTNLS
                     RDHLDFHPTMQDYFDAKARLFDPESRTHASISVICIDDDAGRTMARLAHHPVSVSATG
                     RDADWRVETIETVELGAQEFVAADPAGVHHGLRVGLPGRYNVANCLLAVALLDAVGVS
                     PEQAAPGLRSATVPGRLEPIDRGQGFLALVDYAHKPGALRAVLETLRAQGSGRLAVVF
                     GAGGNRDPGKREPMGRVAAELADLVVVTDDNPRDEEPAAIRAAIMAGAQGGPAQVIEV
                     GDRREAIDHAVAWARPGDVVLIAGKGHEAGQTSHGHTRPFDDREELAAALGARA"
     gene            complement(4372..>6191)
                     /locus_tag="VFC01_20985"
     CDS             complement(4372..>6191)
                     /locus_tag="VFC01_20985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZN82136.1"
                     /translation="AENGLRSASFVFRHRTGNAVIFLVLLVATAQLFNLQVPLAAGLR
                     AEAAGQLKVTDVEKAVRGAIVDRNDDKLAFTIEARALTFQPVKIRKQLEEAKAKTPQA
                     PDPQRRLRDIAAEVALRLNNKPDAGTVLKKLTGNDSFVYLARAVDPAIADAITTKFPE
                     VGSERQDLRQYPGGSLAANIVGGIDWDGHGLLGLEDSLDAVLAGTDGSITYDRGSDGV
                     VIPGSYRNRHDAVDGSTVMLTLDDDIQFYVQQQVQQAKDLSGSKNVSAVVLDAKTGEV
                     LAMANDNTFDPSQDIGRQEDRELGNPAVSSPYEPGSVNKIVTAASVIELGLSNPDEVL
                     QVPGEIDMGGVSVHDAWEHGVTPYTTTGIFGKSSNVGTLMLSQRIGPERYADMLDKFG
                     LGQRTGVGLPGESAGLVPPIDQWSGSTFSNLPIGQGLSMTLLQMTGMYQTIANDGVRI
                     PPRIVKTTIAPDGTRTDEPRPEGVRVVSPQTAQTVRQMLRATVQRDPGGDQQGTGPLA
                     AVDGYQIAGKTGTAQQINPGCGCYSDNVYWITFAGVAPAEDPRYVIGIMGDNPQRTAE
                     GLQGTTMAPLFHNIAAWLLQRENIPLSPDPGPPLTLQAT"
BASE COUNT          953 a         2126 c         2130 g          982 t
ORIGIN      
        1 cgcagtgggc ggcagcccgg gactggtgac gacgaggtcg tagtcggtga tccggtcggc
       61 ggcgttggcc gcgtcgagca ccgcgacgcc gtgctgcgcg aactccgtca gcgccgccgg
      121 gctgtcgtcg gtgagcgtgg cacgagcacc gagtggcgtc agcgcggcca ggatggcgcg
      181 cccggtgacg cgggcccccg cgaccagaac cttggctccc gacaagtcca cgtcaggcac
      241 cgactgtggt cagccactcg ctgtagaaga gcgcgacacc gaggccgcat gcgatcgcgg
      301 tgaggagcca gaaccggatg atgaccgtgg tttccgccca gccgacaagt tcgaagtggt
      361 gatggaacgg cgccatccga aacacccggc gcccggtggt gcgaaacgcc atgatctgga
      421 cgacgaccga ggtcacctcg gcgacgaaca gcgcaccgag cacgaccgcc agcatctccg
      481 tgcggctggt caccgaaagt ccggcaatga tgccgccaag tgccagtgag ccggtgtcgc
      541 ccatgaagat cttggccggc gcggcgttcc accatagaaa gccgatgcac gcccccgccg
      601 tggccgccgc gatgatcgcg agatccaacg gatcgcggac gttgtagcag cccagccccg
      661 ggctggtcgc gcaggcgttg cggaactgcc agaacgtgat cagcacgtag gcggcgcaca
      721 ccatggccat cgccccggcc gccaggccgt ccaggccgtc agtgaagttc accgcgttcg
      781 accaagcgct gacgatgacg atgcagaaca gcacgaacac tgcgggggcg agggtgaccg
      841 tcgcgatctc gcgcacgtac gacaactcgg cgctgccagg tgtcagcccg tcggcattgc
      901 ggaactgcag ggccagcacc ccgaacagca cggcggcggt cagaatgccg acggtcttgg
      961 cggtcttgtt caggccgagg ttgcgcgagc gccggatctt gatcaggtcg tcgacgaaac
     1021 cgacggcgcc caacgcggtc gcaaggccga gcaccagcca gcccgaggcg gacggcccct
     1081 tgccgtccat cacgatgccg atcaattggg tgcccagata gcccgcccag atgcctgcga
     1141 cgattgcgac gccacccatc gacggggtgc cgcgcttctt gtggtggctc ggcggcccgt
     1201 cctctcggat ctcgtgtccg aacccctgcc gggtgaacag ccggatcagc accggcgtga
     1261 gaaggatcga gatcgtcagc gcgatgccga cggcgatgag gatctgcctc atccgcgaac
     1321 gtcctcgtcc gccagggcgt cggcaagggc accgagcccg gccgcattcg aggctttcac
     1381 cagcaccaca tctcctgcgt gcagttcggc ccgcagcagt gcgagcgcgg cgttggcgtc
     1441 gtcaaccatg gtggattcgg ggccccacga cccctccatg accgccccgt ggtgcatggc
     1501 gctcatagac ctcccggttc ccacgacgat gagtcgtgac acatctaatc gcaccgccaa
     1561 ccggccgatg cggtcatgct cggatatcgc gtcgtcgcca agctcggcca tctcgccgag
     1621 cacggcccag ctgcggcgct tcgccccgcc ctcgtgcgcg atggcggcca gcgccttcag
     1681 gcccgcccgc atggagtccg ggttggcgtt gtaggcgtcg ttgatgaccg tgacgccgtc
     1741 gtcgcgggtg gtgacctgca tgcggtgttt ggacgccgga cccgccgctg ccaacgccgt
     1801 ggccacctgt tggagactgg cgccgcactc cagcgcgacg gcggcagagc acagcgcgtt
     1861 cgatacttgg tggtcgccgt gcacggccag tgcaacgtcg gtctggccat cggcggtatg
     1921 caatgtgaac cgcgcccggg ccagcgcgtc gagcgtgacg ggcccggccc acacatcggc
     1981 gtccggcgtg cgacccacgc gcaccacccg cgccgccgtc tggtcggcca tcgcggccac
     2041 ggcggtgtcg tcgacgttga ggatcaccac tcctgacgac ggaacagctt gcgggagttc
     2101 ggctttcgtc gcggcgatgg cctcgcgaga gccgaattca ccgagatgcg cggtgccgac
     2161 gttcagcacg accgcgatcg acggcggcgc gatcgatgcg agtgcggcga tgtttccagg
     2221 gtggcgagcc gacatctcga ggaccaggta gtcggtttcg gtggtggcgc gcagcaccgt
     2281 ccacgggtgg ccgagctcgt tgttgaagga gcccggcggg gcaacgactt cccccagcgg
     2341 gccgagcacc gcggcgagca gatctttggt cgacgtctta cccgacgagc cggtgatgcc
     2401 gacgatggtc agcccaccgt cgacgagttc ggcggctacc gcggacgcga gccgcgccag
     2461 cgccgcgagc accgcggcgc ccgagccgtc ggtgtcgtgt tcgagcacgc cggccgcggg
     2521 gtcgtgggct agttcaggtg cgacgacgat cgcgggcacg cccactggcc gcgccgccag
     2581 caccgcgacg gcgcccgcac ccacggcagc ggcggcgaag tcgtggccgt cggagcgtgc
     2641 ccccggcagc gccaggaata ggccgccggg agtgactgcg cgcgaatcga attcgacggt
     2701 gccggtgacg cgcgtcgctg ccgcgtcgtc ggaggaaata tcggtgagcg ttccgccgac
     2761 gatgtcggcg atctgggcga cggtcagatc gatcacgcgc gggcccccag cgcggcggca
     2821 agttcctcgc gatcgtcgaa gggccgcgtg tggccgtggc tggtttggcc ggcctcgtgg
     2881 cccttgccgg cgatcagcac cacgtcgccc ggtcgcgccc aggccaccgc gtggtcgatg
     2941 gcctcgcgac ggtcgccgac ctcgatcact tgcgccgggc cgccctgggc gccggccatg
     3001 atcgcggccc tgatggcggc gggttcctcg tcgcgaggat tgtcgtcggt gacgacgacc
     3061 aggtcggcga gttcagcggc cacgcggccc atcggctcgc gcttgcccgg atcccggttg
     3121 ccgcccgcac cgaacaccac ggcgagccgg ccactgccct gcgcccgcag cgtctcgagg
     3181 accgcccgca aagcccctgg cttgtgcgcg tagtcgacaa gcgcgagaaa gccctgaccg
     3241 cggtcgatcg gctcgagccg gccgggcacc gtcgcgctgc gaagccccgg cgccgcctgc
     3301 tccggtgaca ccccgacggc gtcgagcagt gcgaccgcca gcaggcaatt ggcgacgttg
     3361 tagcggcccg gcagcccgac ccgcagcccg tggtgcacgc ccgccgggtc cgcggcgacg
     3421 aattcttggg ccccgagttc gaccgtctcg atggtttcca ctcgccagtc ggcgtcgcgt
     3481 ccggtcgcgc tcacggacac cggatggtgt gcgaggcggg ccatcgtacg ccccgcgtcg
     3541 tcgtcaatgc agatcacgga aatgcttgcg tgcgttcgcg actcggggtc gaacagccgt
     3601 gccttcgcgt cgaaatagtc ctgcatcgtc gggtggaagt cgaggtgatc tcgcgacagg
     3661 ttggtgaatc ctccgacctc gaagtgcacg ccgtcgacgc ggcccagtgt cagggcgtgg
     3721 ctggacacct ccatcaccac agtgtcgacg ccctgttcga ccatcaccgc cagcagggcc
     3781 tgtagatcgg gtgcctccgg tgtggtcagg gcgctgggct gatcgcgccc gtcgatgcga
     3841 actccgacgg tgccgatcag cccggccacc cgctcggccg agcgcaggcc ggcctccacc
     3901 agatacgtcg tcgtggtctt gcccgacgtt ccggtgacgc cgatgacgcg caaccgatcc
     3961 gatggatgtc catagacggc cgccgccaac tcgccaagga ccgaacgcgg gtcgggatgg
     4021 atgagcaccg gcaccccgac atcgccaccc atcgcgtcga cgcctgcggg gtcggtcagc
     4081 accgcgacgg cgccgtgagc caccgcgtcg gcggcatagc gaccgccgtg cgacgacgac
     4141 cccggcagcg ccgcgaacag gtcgcccggc cgcgcgtctt gaccccgcag cgtcacgccg
     4201 atgacgtgca cgtttggcac cgcactgctc ccggcgggca tggcgccgac ctgctcggcc
     4261 agccgcccca gcgcttggcc gacgggatcg ttgggacgca ggttcatggc tttgacacag
     4321 taccggcctg gcttttggtg tcccctcgca agctggggac cccaaccgcg gtcaggtggc
     4381 ttgcagtgtc agcggcggac ccgggtcggg cgacagcggg atgttctccc gctgcagtag
     4441 ccaggcggcg atgttgtgga acagcggcgc catcgtggtg ccttgcaggc cctcggcggt
     4501 gcgttgcggg ttgtcgccca tgatgccgat gacgtaccgg ggatcctcag ccggggccac
     4561 gcccgcgaac gtgatccagt agacgttgtc ggagtagcag ccgcagccgg ggttgatctg
     4621 ctgggcggta ccagtcttgc ctgcaatctg atagccgtcg acggccgcca gcggcccggt
     4681 gccctgctgg tcgccccccg gatcgcgttg caccgtcgcg cgcagcatct gccgcacggt
     4741 ctgcgcggtt tgcggcgata ccacccgcac gccctccggc cgtggctcgt cggtgcgagt
     4801 gccatctggc gcgatcgtgg tcttgacgat gcgcggcggg atgcgcacgc catcgttggc
     4861 gatggtctgg tacatgcccg tcatctgcaa cagcgtcatc gaaaggcctt ggccgatggg
     4921 caggttcgag aaggtactac ccgaccactg gtcgatcggc ggcaccaggc ccgcgctctc
     4981 gccgggcagc cccaccccgg tgcgctgacc aaggccgaac ttgtcgagca tgtcggcgta
     5041 gcgttccggg ccgatccgct gcgacagcat cagggtgccc acgttcgacg acttaccgaa
     5101 gataccggtc gtggtgtacg gcgtcactcc gtgttcccag gcgtcgtgga cgctgacgcc
     5161 gcccatgtcg atctcaccgg ggacctgcag cacctcatcg gggttggaca ggcctaattc
     5221 gatcaccgag gcggcggtga cgatcttgtt gaccgagccc ggttcatacg gcgacgagac
     5281 ggccggattg ccgagttcgc gatcctcctg gcggccgatg tcttgcgacg ggtcgaacgt
     5341 gttgtcgttc gccatcgcaa gcacctcacc ggttttcgcg tccagcacga cggctgacac
     5401 gttcttcgat ccggacaggt ccttggcctg ctgcacctgc tgttggacgt agaactggat
     5461 gtcgtcgtcg agggtcagca tcacggtcga accgtcgacg gcgtcgtgcc ggttgcgata
     5521 gctgcccggg atcaccacgc cgtcggagcc gcggtcatat gtgattgatc cgtcggtgcc
     5581 cgccaacacg gcgtccagtg aatcctcaag tccgagaaga ccgtggccgt cccagtcgat
     5641 tccgccgacg atgttggccg ccagcgatcc cccggggtac tggcgcaaat cctgccgctc
     5701 cgaaccgact tcggggaact tggtggtgat cgcatcggcg atggccggat cgacggcgcg
     5761 ggcgaggtag acgaacgagt cgttgccggt gagcttcttc agcacggtgc cggcgtcggg
     5821 tttgttgttg agccgcaacg cgacttcggc ggcgatatca cgcagccgcc gctgggggtc
     5881 cggcgcctgc ggcgttttcg ccttggcctc ttcgagctgc ttgcggatct tgactggctg
     5941 gaaggtcagc gcgcgcgcct cgatggtgaa ggccagcttg tcgtcgtttc ggtcgacgat
     6001 cgcgccgcgc acggccttct ccacgtcggt gaccttgagc tgacccgccg cctcggcacg
     6061 caggcccgca gcgaggggca cctgcagatt gaacagctga gcggtggcga ccagcagcac
     6121 caggaagatg acggcgttgc cggtccgatg ccggaatacg aacgacgcac tgcgcaaacc
     6181 attttcggct a
//
LOCUS       DASRJA010000479         5552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1074818,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000479 DASRJA010000000
VERSION     DASRJA010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074818"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFC01_20990"
     CDS             complement(<1..432)
                     /locus_tag="VFC01_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014385221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN82137.1"
                     /translation="MRGRTDELKVIGAMVTALTHGRGGVLVIEGPPGIGKSRLLTEVW
                     ALAGKRGVRTLFGEAFEYQQTVPFFSLFMATLHSNPPVGDAEALRQLGGSADLPYWVV
                     HDLADAIRAAAEKTPVAIILEDIHWADNGTLLALRSLATAPD"
     gene            complement(579..3356)
                     /locus_tag="VFC01_20995"
     CDS             complement(579..3356)
                     /locus_tag="VFC01_20995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN82138.1"
                     /translation="MTQPSAISPIRGRTEELKVIQALVSAVANGRGGVLLIEGPPGIG
                     KSRLLTEVRAVAEKYGVRGLFGEAFEYQQTAPFFSLFTATLHADPPVGDADALRRLGS
                     SSDQRYWVVHDLARALGAAAAETPLAIMLEDVHWADSGTLLALRSLASARPDLPVLWV
                     LTARTGAGGAPVQETLSVLQRANATVMRIGAMSPDAVADMVQDRVRAKADASLLTLAA
                     KAHGNPFLVNELVCGLDEEGRLTVSGGRAAAAGDELPRRLGATMQQRLDLLAHNAGEL
                     VRVAALLPDRFAASLLAAMVGRPPVSLISAIEEAVRADLLVEDGECLRFRHDLLREAT
                     RQSIPQSLRRAMERQSASIMLGMGVAPAEVATQLARSAEPGDAEAVSALRQAARSVGQ
                     CDPSAAADFSKRALELLPANDVDHGSLVADTVALLNGASRYREAEELAAAALLQAASS
                     RDEAEIRLRRSAVTKHCTQQRVEENRRALQLRDIGDVTRARHLAWLAYNLLYDESGHQ
                     RAAADEAASAAAATGDDEAGILVAVTLACLDGGDGYAGRALQRIEEAYAIASASVATA
                     AHLVAASNYANLLAVLGRLDDARAVVARGAERAHREGSAMAHAVWATFGGIVHLAAGR
                     LSDARAAIEFMPRPEPAASTELDTLCMIILAEVAMRTDDRKLLQQMVNHAREAHVTGS
                     SKVQRGAAYLLALAAWHRSDIDEATRWLGGDMSVLGTPLVPQTLDRVILSARVASAAG
                     DAGLRAAVLRASDVLEREHPVNLLFTGVAAYARGILGRDVSGLVAAAELLESSSRPLF
                     YAAAAEDAGAELARGGRGDEAIAQLNAAFDAYVRHGALADARRVTRDLRRLGVERRVV
                     SPRAKSGWDSLTDSELKVVNLIAQGATNRDVATQLHLSIHTVKTHAHNAFAKLGITSR
                     EQLAQRVREAA"
     gene            complement(3409..4425)
                     /locus_tag="VFC01_21000"
     CDS             complement(3409..4425)
                     /locus_tag="VFC01_21000"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82139.1"
                     /translation="MSIESRATGRDQWLRLYPIALGLGGLGGAWRTAVGFGAPAWPAV
                     GLAALCLGVWTMVTVMYVVTGRGDPRAFIADLRHPDQGFAVVYLPLIPLLLLAHAPRT
                     RVFYVLDLAVGAVWAIATAALVAHWITTPRERHAIHPGFSLPVIGGPFIACVSLQANG
                     WHSLAQAIFAIGVFFWMMFGTLIVGRLMTEERLSDARFPTLAILMVPPATASIGWFAL
                     NDNRVTTVGTGLAGVLAMMALVQLFILPEYLRRPFTISAWSFSFPLASTANTVCHWAI
                     AAPNPESRLFAWATLVIATVIIGLLAALTVRKVWMSHLLRSPSPSEIRSDASNVAMAA
                     AAEL"
     gene            complement(4439..4891)
                     /locus_tag="VFC01_21005"
     CDS             complement(4439..4891)
                     /locus_tag="VFC01_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82140.1"
                     /translation="MSTIHFHQTTTSTPEQFVAGLTDFGPGRAKLFSNSADSYLKVHH
                     QGVREADVTEGSSGIWERLQYDWSDPNHVKLTTTDSNVFGGASGYTYTLSPLPNGMTD
                     VDVVIVRQGKNLKGRVLSGVLGTVGKGSLRKAFVKSVKAIEARGAAVS"
     gene            complement(5023..>5552)
                     /locus_tag="VFC01_21010"
     CDS             complement(5023..>5552)
                     /locus_tag="VFC01_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZN82141.1"
                     /translation="SARRDNAGVEALPDGTQELIDQFAMPAIVASRCLDVLAANAMAR
                     ALSPGFAPGQNFLRWRLLEPAARELYVDWDEATDVAVSGLREAAGSDPDDPRLQSLID
                     ELSAASERFRELWARAEVGYRVGVLHMRHPKVGDLHLHRYRLNIPHSGGQHMLIYHAE
                     PGSDSARALEALRST"
BASE COUNT         1006 a         1888 c         1744 g          914 t
ORIGIN      
        1 gtccggcgcg gtggccagcg agcgcagcgc caacagtgtg ccgttatcgg cccaatgaat
       61 gtcctccaaa atgattgcta caggcgtctt ctcggctgct gcacgaatcg catccgccag
      121 atcgtgcacc acccaatagg gcagatctgc cgagccgccg agctggcgca gcgcctccgc
      181 gtcgccgacc ggggggttcg agtgcagtgt ggccatgaac agcgagaaga acggcaccgt
      241 ctgctgatac tcgaacgcct caccgaacaa ggtgcgcacc ccacgcttgc cggccagcgc
      301 ccacacctcg gtcagcaagc gactcttacc aatgcccggt gggccttcga tgaccaacac
      361 gcccccgcgc ccgtgcgtca gcgccgtcac catcgcgccg atcaccttca gctcgtccgt
      421 ccgcccacgc atgggcggag tgacgaaccg ttcgccggcg acattcgata ggccggttga
      481 aggccccacg ggtgtcctgt ctaccgtttg ctggactgcc aatgcagtgt gagtaattct
      541 aggcgtcagc ccacgtcatg cgcccgattt gcggtacatc acgcagcttc gcgcacacgt
      601 tgcgccagct gttctcggga ggtgatgccc agcttggcga acgcgttgtg agcatgggtt
      661 ttcaccgtat gaatggatag atgcagctga gtcgcgacgt cgcggttggt cgcgccttga
      721 gcgatcaggt tgaccacctt gagctcggag tcggtgaggc tgtcccagcc cgacttcgcc
      781 cgagggctta cgacccgacg ttcgacgccc aaccggcgca agtcgcgggt cacacggcgc
      841 gcatcggcga gggctccgtg acgcacgtaa gcgtcgaaag cggcgttgag ttgagcaatc
      901 gcctcgtcac cacgaccgcc gcgcgcgagt tcggcgccag cgtcttcggc agcggcggcg
      961 tagaacagtg gccgcgacga cgattcaagc aactccgccg cggcgaccaa ccccgagaca
     1021 tccctgccga gtattccgcg cgcatacgcc gccacaccgg tgaagaggag gtttaccgga
     1081 tgctcacgct ccaggacatc gctagcccga agcacagccg ctcgcaggcc ggcgtccccg
     1141 gccgccgacg ccacccgagc gctcaagatg acccgatcca gtgtctgcgg cacgagtggg
     1201 gtgccgagca cggacatgtc gccgccgagc cagcgcgtcg cctcatcgat gtcactgcga
     1261 tgccatgccg ccagcgcgag cagatacgct gctccgcgtt gcactttgga ggatccggtg
     1321 acgtgagcct cgcgcgcatg attgaccatc tgctgcaaca gcttccggtc atcggtgcgc
     1381 atcgcgacct cggccagaat gatcatgcaa agcgtgtcca gctcggttga ggccgccggc
     1441 tccgggcgag gcatgaactc gatcgcggcg cgcgcgtcgg acaaccggcc ggcagccagg
     1501 tggacgatcc cgccgaatgt ggcccagact gcatgagcca tcgcgctgcc ttctcggtgg
     1561 gcccgctcgg cgcctcgtgc gacaacggcc ctcgcgtcgt ccaaccgccc gagcaccgcc
     1621 agcaggttcg cgtagttgct cgcggccacc agatgcgctg cggtcgcgac gctggcgctg
     1681 gcgatcgcgt atgcctcctc gatgcgctgt agcgcgcgcc cggcataccc gtcgccaccg
     1741 tcgaggcaag ccagggtgac ggcaaccagg atccccgcct cgtcatcgcc ggtggctgca
     1801 gccgccgagg cggcctcgtc ggccgcagca cgctggtggc cactttcgtc gtagagaagg
     1861 ttgtatgcca gccaggcgag atgccgcgcc ctggtcacgt cgccaatgtc gcgcagctgc
     1921 aatgcgcggc ggttctcctc cacccgctgc tgggtgcagt gcttggtgac cgcggagcgc
     1981 cgcagccgga tctcggcctc atctcggctt gacgccgcct gcaagagcgc tgccgcggcc
     2041 aattcctctg cctcccgata gcgactggct ccgttgagca acgccacagt gtcggccacc
     2101 agtgacccgt ggtcaacatc gttggcgggc aatagctcca atgcacgctt gctgaagtcc
     2161 gccgccgcac tcggatcgca ttggcccact gaccgcgcgg cctgccgcag tgcgctcacc
     2221 gcttcggcgt ctcccggctc ggcgctgcgc gccagctggg tggccacctc ggccggcgcc
     2281 acccccatcc ccaacatgat cgacgccgac tgacgctcca tcgcacgacg tagcgactgc
     2341 ggaatggact gtcgggttgc ctcgcgcagc aggtcatgtc gaaacctcag gcactcacca
     2401 tcttccacga gcagatcggc gcgcactgcc tcctcgatgg ccgaaatcaa cgacaccggc
     2461 gggcgaccga ccatcgcggc cagaagcgat gccgcgaacc ggtcgggcaa tagcgcggcg
     2521 acccgcacca gctcgccggc attgtgggcg agcaggtcga gtcgctgctg catggtggcg
     2581 cccaggcggc gcggcagttc atctccggcg gccgccgcac gcccgccact gacagtcagc
     2641 cggccctcct cgtccagacc acagacaagc tcgttgacca ggaacggatt cccgtgcgcc
     2701 tttgccgcga gagtcagcag cgacgcgtca gctttcgccc gcacacgatc ttggaccatg
     2761 tcagcgaccg cgtccggcga catcgcaccg atccgcataa ctgtggcgtt cgcgcgttgg
     2821 agtaccgaca gtgtctcctg gaccggtgct ccaccggcgc ctgttcgagc cgtcagtacc
     2881 cacagcaccg gcaggtccgg cctcgcgctg gccagcgacc gcagtgccaa cagcgtgccg
     2941 ctgtcggccc aatggacatc ctccaacatg atcgccagcg gcgtttccgc agcagctgcg
     3001 ccgagcgccc tcgccaggtc gtgtaccacc caataacgct gatcggacga actgcccagt
     3061 cggcgcaacg catcggcgtc cccgaccggg gggtcagcgt gcagcgtagc cgtgaacaat
     3121 gagaagaagg gcgcggtctg ctgatactcg aacgcctcac cgaacaagcc acgcacacca
     3181 tacttttcgg ccacagcgcg cacttcggtc aacaaccgac tcttgccgat gcccggcgga
     3241 ccttcgatga ggagaacccc gccgcgacca ttcgccaccg cggaaaccaa cgcttggatg
     3301 accttcagtt cttcggtccg gccgcggata ggtgagatcg ccgacggctg cgtcatcaag
     3361 cgcatctccg ggccgagtcg ttgtgccagt gtcacggtat tcccaccttc aaagttctgc
     3421 cgcggcggcc attgcgacgt tgcttgcatc ggaacggatt tcactcggcg atggcgaccg
     3481 gagtaagtgg ctcatccata ccttgcgcac ggtgagcgca gcgagcagcc cgataatcac
     3541 ggtggcgatc accagcgtcg cccaggcgaa cagtcgactc tcaggattag gtgccgcaat
     3601 cgcccagtgg cataccgtgt tcgctgttga tgccaacggg aagctgaacg accacgcaga
     3661 gatcgtgaac gggcggcgga ggtactcagg gaggatgaac agctggacga gcgccatcat
     3721 cgccagcacg cccgccagtc cggtgccgac cgtggtgacc ctgttgtcgt tcaacgcaaa
     3781 ccagccgatg ctcgcagtgg ccgggggaac catcaagatc gctaacgtgg gaaagcgcgc
     3841 gtcgctgagg cgttcctcgg tcatcaggcg gccaacgatt aaggtgccga acatcatcca
     3901 gaagaacacg ccgatggcga agatcgcctg cgcgagagaa tgccagccgt tggcctgcag
     3961 cgaaacacag gcgatgaagg gtccgccaat aacgggcaac gagaagccag gatgtattgc
     4021 gtggcgttcc cgcggcgtcg tgatccaatg cgccacaagc gctgccgtcg caatggccca
     4081 caccgctccc acggcaaggt caaggacgta gaaaacccgt gtgcgagggg cgtgggccaa
     4141 gagcaacagc ggaatcaagg ggaggtacac caccgcgaag ccctggtccg ggtggcgcaa
     4201 gtcggcgatg aacgcccgtg gatccccgcg acccgtcacc acgtacatga cggtgaccat
     4261 ggtccagacg ccgaggcaca gcgccgccaa gcccactgcc ggccaggctg gggcgccgaa
     4321 accgacggcc gtgcgccacg ccccgcccag accacctagg ccgagcgcaa tcgggtacag
     4381 gcgcaaccat tggtcacgtc cggtggcgcg agattcgatc gacacggttc gacagttcct
     4441 acgagactgc cgcgccgcgg gcttcgatgg ctttgacgct cttgacgaac gcctttcgca
     4501 atgaaccctt gccgacagtg ccgagtacgc ccgagagcac ccgccctttg aggttcttgc
     4561 cctgtcggac gatgacgacg tcgacgtcgg tcattccgtt gggcagtggg ctcaaggtgt
     4621 aggtgtagcc cgatgcgcca ccgaacacgt tcgagtcggt ggtcgtgagc ttgacgtggt
     4681 tgggatcgga ccagtcgtat tgcagacgct cccagatacc gcttgacccc tcggtgacgt
     4741 cggcctcgcg gacaccctgg tgatgcactt tgaggtagga gtcggcgcta ttcgaaaaga
     4801 gcttggcgcg gcctggcccg aagtcggtga gtcccgcgac gaattgctct ggggtcgaag
     4861 tggttgtctg gtggaagtga atggtggaca tgagaaactc cgttccataa cgcttttcgt
     4921 gcgatgtcgc actccatcag cgcgcaggag gtgttcctga ttccggcaac cggactgtca
     4981 gcgtgacccg agtgatcctg gtagtgacgg gaacagcctt cgtcaggtcg accgaagtgc
     5041 ctcgagtgct ctggccgagt cgctgccggg ttcagcgtga tagatcagca tgtgctgccc
     5101 gccggagtgc gggatgttaa gccggtagcg gtgcaggtgc agatctccca ccttcggatg
     5161 gcgcatgtgc aggacgccca cccgataacc cacctccgcg cgcgcccaca attcgcggaa
     5221 tcgctcactg gcggccgata attcgtcgat cagcgactgt aagcgcgggt cgtcggggtc
     5281 gcttccagcc gcctcacgca gcccgctgac cgcgacgtcg gtcgcctcgt cccaatcgac
     5341 gtacagctcg cgtgcggccg gctctaacag tcgccagcgc aggaagtttt gccccggcgc
     5401 aaacccaggc gacagggccc gcgccatggc attggccgcc aatacatcga ggcaccggct
     5461 cgccacgatc gccggcatcg caaactgatc gatcagttcc tgtgtgccgt cgggcagcgc
     5521 ctcaacgccc gcattgtcgc gtcgggcgga gg
//
LOCUS       DASRJA010000480        15858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3088250,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000480 DASRJA010000000
VERSION     DASRJA010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15858
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3088250"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..2564)
                     /locus_tag="VFC01_21015"
     CDS             complement(231..2564)
                     /locus_tag="VFC01_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HZN82142.1"
                     /translation="MTTTEDAGLEKPRIKAPKRSAAGIPAVMHALEYSLEQTSLVRTA
                     INLLSINQTNGIDCPGCAWPEPEPGKRHKNEYCENGAKHINDEATTRRITRKFFRDHP
                     ISELAAKSDMWLNEQGRLTEPMVKRPGSDYYEPIGFHDAFGLLAAELRGLDSPDEALF
                     YTSGRLSNEAAFLLQLFARAYGTNNLPDCSNMCHESSGAGLGETLGVGKGSVSLDDLH
                     EADLVLAVGLNPGTNMPRMLSALEETKRNGGHVIAVNPLPEAGLIRFKNPQKVSGILG
                     RGTAIADQFLHIKPCGDLALFQAINALLLEAEDAAPGRVLDFEFIDTHTVGFAEFADH
                     ARQVSWSDVLDATGLSRHEIEQVAERVRASKKVVVCWAMGVTQQKHGVATVREIVNFL
                     MLRGNLGRPGTGVCPVRGHSNVQGDRTMGIWEKMPQSFLDALEQEFGFTPPAKHGLDA
                     VDSIRAMRDGRAKLFLGFAGNFVRATPDSEVTEQAMRNCRLTAHISTKLNRSHAVCGE
                     TALVLPTLGRSDLDIQATGEQFVTVEDSMSIVHQSRGRLTPASEHLLSEVAIICRLAR
                     RTLGETPEIPWEKFESDYDVIRDAISRVVPDFDEFNDRVRNPGGFTLPNPVNDYRFNT
                     ASGKAQFTCNTFDRIQVPPGHLVLQTMRSHDQWNTVPYAPNDRYRGIRNGRRIVLINP
                     DDIEELGLVDRQLVDLVSTWEDGVERRAEDFRVIAYPAARGSAASYYPETNVLVPLDS
                     VADISNTPTSKGVFVRFEPVKDHGRASEVTNAMTATG"
     gene            complement(2588..4012)
                     /locus_tag="VFC01_21020"
     CDS             complement(2588..4012)
                     /locus_tag="VFC01_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZN82143.1"
                     /translation="MNYLVVLLYLVSFGMFIYGLMGLTGPKTAVRGNWIAAAGMGLAV
                     IATLIAIRHTENWGLIIAGLLVGVALGVPPARLTKMTAMPQLVAFYNGVGGGTVALIA
                     LSEFIDTSGFSAFQHGESPTAHIVVASLFAAIIGSISFWGSIIAFGKLQEILPGRPIG
                     VGKLQQPLNILLAVAAIAAAVVIGINAHPGSGGASLWWMIGLLAAAAVLGLMVVLPIG
                     GADMPVVISLLNAMTGLSAAAAGLALNNTAMIVAGMIVGASGSILTNLMAKAMNRSIP
                     AIVAGGFGGGGVAVGGGDDGDKVVKATSAADAAIQMAYANQVIVVPGYGLAVAQAQHA
                     VKDLATLLEDKGVAVKYAIHPVAGRMPGHMNVLLAEAEVDYDAMKDMDDINDEFGRTD
                     VTIVIGANDVTNPAARNDPSSPIHGMPILNVDQSKSVIVLKRSMNSGFAGIENPLFYA
                     DGTTMLFGDAKKSVTEVAEELKVL"
     gene            complement(4009..4368)
                     /locus_tag="VFC01_21025"
     CDS             complement(4009..4368)
                     /locus_tag="VFC01_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZN82144.1"
                     /translation="MYDQLLANLAILVLSGFVGFSVISKVPNTLHTPLMSGTNAIHGI
                     VLIGALVVLGRVEHPTLAEQIILFVAVVFGTLNVVGGFVVTDRMLGMFKGKKPAKAAA
                     APAESAAAAAGTNGQAR"
     gene            complement(4387..5484)
                     /locus_tag="VFC01_21030"
     CDS             complement(4387..5484)
                     /locus_tag="VFC01_21030"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005078757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HZN82145.1"
                     /translation="MTEQTTVGVVAESGSDERRVALVPKAVASLVNKGLPVVVESGAG
                     DAALLPDDLYIEAGATIGDAWRADVVVKVAPPTADEVGKLKSGQTLIGFLAPRNADNQ
                     IDALKSAGVQAFALEAIPRISRAQVMDALSSQANVAGYKAVLVAASESTRFFPMLTTA
                     AGTVKPASVLVLGVGVAGLQALATAKRLGGKTTGYDVRPEVADQVRSVGAQWLDLGIE
                     AAGEGGYARELSDEEKAEQQKKLEEAITGFDVVITTALVPGRPAPRLVTATAVEGMKP
                     GSVVVDLAGETGGNCELTEPGQTVVKHGVTIASPLNLPATMPEHASELYSKNITALLD
                     LLVKDGALAPDFDDEVIAESCVTRAASGAES"
     gene            5547..6686
                     /locus_tag="VFC01_21035"
     CDS             5547..6686
                     /locus_tag="VFC01_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN82146.1"
                     /translation="MAVDRLLPSQDAEDLIALTRDIADKVLDPIVDAHEKAETYPEGV
                     FGQLGAAGLLSLPQPEEWGGGGQPYEVYLQVLEEIAARWAAVAVAVSVHSLSSHPLLA
                     FGTDEQKQRWLPGMLSGEQIGAYSLSEPQAGSDAAALQCKATKSADGYVLNGSKSWIT
                     HGGRADFYTLFARTGEGSRGVSCFLVPGDLAGLSFGKPEEKMGLHAVPTTSAFYDHAP
                     IEADRLIGAKGQGLQIAFSALDSGRLGIAAVAVGLAQAALDEAVKYANERTTFGRKII
                     DHQGLGFVLADMAAAVASARATYLDAARRRDLGRPYSQQASVAKLVATDAAMKVTTDA
                     VQVFGGVGYTRDYRVERYMREAKITQIFEGTNQIQRLVIARGLTT"
     gene            6739..8091
                     /locus_tag="VFC01_21040"
     CDS             6739..8091
                     /locus_tag="VFC01_21040"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN82147.1"
                     /translation="MTIETASTRISSWQRTLTASRVTTAEGLPPGPRLPSALQSVLIW
                     TQRQRFIPAMHRRYGDTFTVNAEPTGFGVVLRDPDDLRTIFRGDPEIFHAGEGNALLA
                     PLVGRRSVLTMDAPDHIEERRRMLPPFHGERIARVVALMEEETEREIAHWPIDQDFAL
                     LPRMQALTLSIIVRVVLGVDEVDRAEAVGAALRRVLEIRLRDMLLWVLPSLQRLRPWS
                     NIVRDLDRADELIYAEIARRRADPARAERPDVLSMLLDGDPDPELVRDELVTLLVAGH
                     ETTAVALSWTFERLLRHSGALSKVQAGLDDPRDPYRAAVAKEALRLRPVIMNIARRLT
                     QPVELAGHMMPAGTLLLPSIIGIHTDPKIWGPDAGYFRPERWLSGDQPTYAWIPFGGG
                     ARRCLGATFALTEIDAVLRTVLRRNELRADRPQDEKPRMHHITLVPSRGARVRVTAKT
                     "
     gene            complement(8141..9343)
                     /locus_tag="VFC01_21045"
     CDS             complement(8141..9343)
                     /locus_tag="VFC01_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN82148.1"
                     /translation="MARPVQQFVDYFAAEPIRVSAILRLPLIGLIAVLVWIWEVDHWL
                     PGLYAVILGVYAAAAALWLVAVLRGPVPRWGDWASTTVDLSVIITLCLVSGGATAALL
                     PVFFLLPISVAFQDRPMMTAIIGTITALGYLAVWIFYSKRDDKVGLPNMVYTHFGFLL
                     WLAVAVTALCYVLARRAARVKALQEMRRQLVSEAMQSDERHNREVAEHLHDGPLQTLL
                     AARLELDEARERNPDPALDMVYAALQDTATGLRSTVTELHPQVLAQLGLTPAVRELLR
                     QFESRTDVAVEADLEDVGKPQSQALLYRAARELLTNIGKHAKATTVRVGLMRKDNRVV
                     LTIDDDGTGFDPAIVAQYVADGHIGLGSLLARFDAMGGSMEIDSRVGYGTQVTATSPP
                     EHSLREQT"
     gene            complement(9345..9986)
                     /locus_tag="VFC01_21050"
     CDS             complement(9345..9986)
                     /locus_tag="VFC01_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN82149.1"
                     /translation="MTSEKVRVVVGDDHPLFRDGVVRALTSSGSIEVVAEADDGPSAL
                     EAIRTHSPQVALLDYRMPGMDGAEVAAAVLRDELPTRVLLVSAHDESAIVFGALQQGA
                     AGFLPKESTRSELVNAVLDCAKGRDVVAPTLAAGLAGEIRKRAEPEGPVLSPREREVL
                     KLIAAGSSIPAMAKELFLAPSTVKTHVQRLYEKLGVSDRAAAVAEAMRRKLLE"
     gene            10163..10402
                     /locus_tag="VFC01_21055"
     CDS             10163..10402
                     /locus_tag="VFC01_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82150.1"
                     /translation="MNTNRFARIIALPIASAGIIGGAALGLAGAASAATTDVQQPNIV
                     AVPSVKAHPAPQAQPGWYWHHGVHHLAELQPSYTR"
     gene            10617..10859
                     /locus_tag="VFC01_21060"
     CDS             10617..10859
                     /locus_tag="VFC01_21060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82151.1"
                     /translation="MTITRSFARYIALPAISAGILGGAALGLAGMANAGTYSYQPTPR
                     PGIVATPNTIARPPVIVIPRHRHHDGGLIINLPAGE"
     gene            complement(10917..11378)
                     /locus_tag="VFC01_21065"
     CDS             complement(10917..11378)
                     /locus_tag="VFC01_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN82152.1"
                     /translation="MTESRPPFPPFTLETARQKVQAAEDAWNTRDPERVSLAYTVDSQ
                     WRNRDEHVVGREQIVAFLTRKWQRELDYVLRKSLWGFRENRMAVRFQYESHDADGQWY
                     RSYGNELWEFTPEGLMARREASINDVKIDESERRYFGPRPESEYGLDIPLW"
     gene            11457..12671
                     /locus_tag="VFC01_21070"
     CDS             11457..12671
                     /locus_tag="VFC01_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN82153.1"
                     /translation="MSAKAQDYHKRLTAFMTEFVFPAEEAYDRYRREAGPEDHTVPPV
                     VEELKKLAKERGLWNLFLPAESGLTNLEYAPLAELTGWSLEIAPEVTNCAAPDTGNME
                     TLHLFATEAQRKQWLEPLLAGEIRSGFSMTEPAVASSDARNIETLITRDGGDYVINGR
                     KWWTTGASDPRCKILIVMGRTNPDAASHQQQSMVLVPVDTPGVNILRSTPVFGWQDQH
                     GHCEIVYDNVRVPVENLLGEEGAGFAIAQARLGPGRIHHCMRALGAAERALALMVDRV
                     QKRVAFGKPLAEQGVVREQIARSRNEIDQARLLCEKAAWTIDQHGNKEAHVLVSQIKA
                     VAPQVACDVIDRAIQVHGAAGVSDDFPLARLYSWHRAMRLFDGPDEVHMRTIARAEIG
                     REKSAFAAAVTS"
     gene            12671..13468
                     /locus_tag="VFC01_21075"
     CDS             12671..13468
                     /locus_tag="VFC01_21075"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HZN82154.1"
                     /translation="MAVDVGELSGAWNFRDVAEETGVRPGRFFRSSELSRLDDSGREV
                     FRQLGITDVADLRSAREVERHGPGLVPEGVEVHLLHFHEVAAVSGEAPHETAFQKMMS
                     EKPDDEDVAVAAHRFMTEEYLRFPTLGGAQRAVRQVISLLADGRPVIAHCFAGKDRTG
                     FTVATVLDAIGVDRDAIMADFLRSNAAVPRLREQILESVRDRSETHEVITFAEARLTD
                     EVLGVQEDYLDASRRSIEQNYGSLKDYLHAAGVAATDIAKARATLLG"
     gene            13572..14198
                     /locus_tag="VFC01_21080"
     CDS             13572..14198
                     /locus_tag="VFC01_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase"
                     /protein_id="HZN82155.1"
                     /translation="MNPDPAHSLVLTTDYRVPDPTRVWPLLQRRRDELAALGAHHVFV
                     FTSTTDPERVLVTIALRTREPVAELLRSRAFFEWFDEVGVDDLPAVFAGELIERFDFD
                     GADTAPPGIVIAGIMVVDDPAAFRLHVRETRDLFQKAGIRRVRIFEAFDDPDEIMFLH
                     ELSDEVSARRWLRRPDVAAEWFSDAGVGAYPPVFIGRIAHMMRISDES"
     gene            14198..14389
                     /locus_tag="VFC01_21085"
     CDS             14198..14389
                     /locus_tag="VFC01_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN82156.1"
                     /translation="MYVCLCLGVTSDTISRAVAAGATTSKQVADACGAGSECGRCRRT
                     VRAIIAATGSDRHQPPRPP"
     gene            complement(14349..14834)
                     /locus_tag="VFC01_21090"
     CDS             complement(14349..14834)
                     /locus_tag="VFC01_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HZN82157.1"
                     /translation="MSTGGIVLVALAIAVGIVGIVVPLLPGTLLVFGAIAVWAVVEHN
                     VTAWVTLGVVTVVVAVSTLIKYLWPMRRMREADVRTMSLAAGAVLGIIGFFVIPVLGL
                     VIGFVLGVYLAELSHRRDQRVAWTSTKHALKGVALSVGVELCGALLATAAWVAGVYLT
                     Q"
     gene            complement(14831..15535)
                     /locus_tag="VFC01_21095"
     CDS             complement(14831..15535)
                     /locus_tag="VFC01_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HZN82158.1"
                     /translation="MTPKALAFDVFGTVVDWRSSVIRELQQFGQRHGLQQDWPSFADD
                     WRAGYAPAMDRVRKGDLPWTRIDDLHRLILEELLGAAGVTSICSEDVDELNMAWHRLD
                     PWPDSVAGLTRLKQKFVITTLSNGNMSLLTNMAKRAGLPWDCVISAELFHHYKPDREA
                     YLGCADLLGVAPDELMLVAAHPSDLKAASAAGLRTAYVDRPLEHGPGSEPHTVGANKF
                     DLTATDFIDLAGKLGA"
     gene            complement(15532..>15858)
                     /locus_tag="VFC01_21100"
     CDS             complement(15532..>15858)
                     /locus_tag="VFC01_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN82159.1"
                     /translation="IGGEAFDDAVRALDTEGRLLVIGFAAGGIPTVKVNRLLLRNISV
                     IGVGYGEYVNRKPGSQSVFEFGVAELVKAGLRPPPPLRFPLAKGAEALQTLADGGVLG
                     KVVLEP"
BASE COUNT         2760 a         5377 c         5011 g         2710 t
ORIGIN      
        1 cccacagcga cacctgatcg cctacagtac gaaacacgat ggcctcaatc ccttcttacc
       61 caagggcttg aggccatcct tcctgttcaa caccgtcaac ccgcggatca acgcgccgac
      121 tttttcaggt cgaagtagct aacgcggcgt ccggcctggt gttgcgcgtt tagccctggc
      181 acgaacgtaa tgcgggccgg agaagcggac gagcgatcgt cggcccgctg ctagccggtc
      241 gcggtcatag cattagtcac ctctgacgcc cggccgtggt ctttcaccgg ttcgaaccgc
      301 acgaagaccc ccttggatgt gggtgtgttg ctgatgtcgg caacactgtc cagcgggacg
      361 agcacattgg tttccgggta gtacgaggcg gccgatcccc gcgcggcggg atatgcgatg
      421 acgcggaaat cctctgcgcg ccgctcgaca ccgtcctccc acgtgctcac caaatcaacc
      481 agctgccggt cgaccagtcc cagctcctcg atgtcgtcag gattgatcag cacgatcctg
      541 cgaccgttgc ggatcccgcg gtacctgtcg ttgggcgcgt acggcaccgt gttccactgg
      601 tcatgcgagc gcatggtctg caacacgaga tgtcctggcg gcacttggat tctgtcgaaa
      661 gtgttgcacg tgaactgcgc cttgcccgac gccgtattga agcggtagtc attgaccgga
      721 ttgggcagcg taaagccgcc agggttgcgc accctgtcgt tgaactcgtc gaagtcgggc
      781 accacacggg atatcgcatc gcggatcacg tcgtagtcgg actcgaactt ctcccaaggt
      841 atctcgggcg tctctccaag cgttcggcgc gcaagcctgc agatgatcgc cacctcgctc
      901 agcagatgct ccgacgccgg ggtgagccgg ccgcgcgact ggtgcacgat gctcatagaa
      961 tcttccaccg tgacgaactg ctcgccggtc gcctgaatat cgaggtcact ccggcccagg
     1021 gtgggcaaga ccagcgccgt ctcgccgcac accgcatgcg agcggttgag ctttgtcgaa
     1081 atgtgcgccg tcagtcggca gtttcgcatc gcctgctcgg tcacctcgct gtcaggagtc
     1141 gcccgtacga aattccctgc gaagcctaga aataacttcg cccgaccgtc ccgcatggcc
     1201 cgaatcgagt cgaccgcatc caggccgtgt ttcgctgggg gagtgaagcc gaactcttgc
     1261 tccagcgcat cgaggaacga ctgcggcatc ttctcccaga tacccatcgt gcggtcgccc
     1321 tggacattgc tgtgcccgcg caccgggcag acacccgtgc cgggccggcc caggttcccc
     1381 cgcagcatca ggaagttcac gatctcgcga accgtcgcca cgccgtgctt ctgttgggta
     1441 acgcccatcg cccaacacac gaccaccttc ttgctggcac gcacgcgttc ggcgacctgc
     1501 tcgatctcat ggcgcgaaag tccggtcgca tcaaggacat ccgaccacga gacctgtcgc
     1561 gcgtggtcgg cgaactctgc gaagcccacc gtgtgcgtgt cgatgaactc gaaatccagc
     1621 acccgtcccg gggcagcgtc ctcggcctcc aataggaggg cgttgatcgc ctgaaacagc
     1681 gccaggtcgc cgcacggctt gatgtgcagg aactgatcgg cgatcgcggt tccccggccg
     1741 aggattccgc tgaccttctg tggattcttg aaccgaatca gacccgcttc gggcaacggg
     1801 ttgactgcga tgacatgccc gccgttgcgt ttggtttcct ccagcgcgga cagcattcgg
     1861 ggcatgttgg tgccggggtt gagtccgacg gcgagcacca gatcggcctc atgcagatcg
     1921 tccaggctca cactgccctt gcccacgccc agcgtctcac ccagtcctgc accgctggat
     1981 tcatggcaca tattgctgca gtcgggcagg ttgttggtgc catatgcgcg cgcgaacaac
     2041 tgcagcagga acgccgcctc gttgctcagc cgcccggagg tgtagaacag cgcctcgtcg
     2101 ggcgaatcga gtccgcgcaa ttcggctgcc agcagaccga acgcgtcgtg gaagccgatc
     2161 ggctcgtagt agtccgaacc gggccgcttt accatcggtt cggtcagccg gccctgctcg
     2221 ttgagccaca tgtccgactt cgccgcgagc tcggaaatcg ggtggtcgcg gaagaacttt
     2281 cgcgtgatcc gacgggttgt cgcctcgtcg ttgatgtgtt tggcgccgtt ctcgcagtac
     2341 tcgttcttgt gccgcttccc gggctccggc tccggccatg cacagcccgg gcagtcgatg
     2401 ccgttggtct ggttgatgct cagcaggttg atcgcggtgc gcacaagcga cgtctgctcc
     2461 agcgaatact ccagcgcgtg catcaccgcg ggaatcccgg cagctgatcg cttgggcgcc
     2521 ttgatccttg gcttctcgag gcctgcgtct tcagttgtgg tcatctgtca caatcttcgc
     2581 tcggccgcta caggaccttg agttcctcgg cgacctcggt gaccgacttc ttggcgtcgc
     2641 cgaagagcat cgtggtgcca tcggcgtaga acaacggatt ctcgatgccg gcgaaccccg
     2701 aattcatcga ccgcttcagc acgatcaccg acttggactg atccacgttg aggatcggca
     2761 tgccatgaat gggtgagctc gggtcatttc gcgccgcggg gttggtgacg tcgttggcgc
     2821 cgatgacgat cgtgacgtcg gtgcgaccga attcgtcgtt gatgtcgtcc atgtctttca
     2881 tggcgtcgta gtcgacttcg gcctcggcca gcagcacatt catgtgcccg ggcatgcgtc
     2941 ccgccaccgg gtggatcgcg tacttgaccg caacgccctt gtcctccaac agagtcgcaa
     3001 ggtccttgac ggcgtgctgc gcctgcgcga cggccaggcc gtaacccgga accacgatca
     3061 cctggttggc gtaggccatc tggatcgcgg cgtcggccgc cgacgtggcc ttgaccacct
     3121 tgtcgccgtc gtcgccgcca ccgaccgcga ccccgccgcc accgaaccca ccggcgacga
     3181 tcgcggggat ggaacggttc atcgccttgg ccatcaagtt ggtcaggatc gaacccgacg
     3241 cgccgacgat catgccggcc acgatcatcg cggtgttgtt cagcgccaaa ccggctgcgg
     3301 ccgcggacaa tccggtcatc gcgttcagca acgagatgac gacgggcatg tcggcgccgc
     3361 cgatcggcag caccaccatg aggcccagca cggccgcggc ggccagcagg ccgatcatcc
     3421 accacagcga cgctccaccg ctgcctggat gcgcattgat tccgatgacg acggccgcgg
     3481 cgatcgcggc gaccgccagc aggatgttca gcggctgctg caacttgccg actccgatgg
     3541 gcctgccggg caggatttcc tgcagcttgc cgaacgcgat gatcgagccc cagaacgaga
     3601 tcgatccgat gatcgcggcg aacagcgagg ccaccacgat gtgcgctgtc ggcgactcgc
     3661 cgtgctggaa ggctgaaaag ccgctggtgt caatgaattc cgacagcgcg atgagcgcga
     3721 cggtgccgcc accgacaccg ttgtagaacg ccaccagctg cggcatcgcg gtcatcttcg
     3781 tcaaccgtgc cggtggcacg cccagcgcca cgccgacgag cagaccggcg atgatcaggc
     3841 cccagttctc ggtgtggcgg atcgcaatca gtgtcgcgat gactgcaagg cccatgcccg
     3901 ccgcggcgat ccagttgccg cgcaccgcgg tcttgggtcc ggtcaggccc atcaagccgt
     3961 agatgaacat cccgaacgag acgaggtaca gcagaacgac caaatagttc atcgcgcttg
     4021 cccgttcgtt cccgcggcgg cagccgcaga ttcagccgga gcggcagcag ccttcgccgg
     4081 cttcttgccc ttgaacatgc ccagcatccg gtcggtcacc acgaaaccgc cgaccacgtt
     4141 gagcgttccg aacaccaccg cgacgaacag gatgatctgc tcagcgagcg tggggtgctc
     4201 gacgcgaccg agcactacca gtgcaccgat cagcacgatg ccgtgaatgg cgttggtgcc
     4261 cgacatcaat ggcgtgtgca aggtgttggg caccttggag atcaccgaga acccgacgaa
     4321 cccggacagc accaggatcg cgagattggc caacaactgg tcgtacattt ttcggactcc
     4381 cccgctctag ctttctgcgc cgctcgcggc gcgtgtcacg caggactctg cgatcacttc
     4441 gtcgtcgaaa tccggtgcca gcgcaccatc tttgacgagc aggtccaaca gcgcggtgat
     4501 gttcttcgaa tacagctcgc tggcgtgttc gggcatggtg gccggcagat tgagcggcga
     4561 cgcgatcgtc acgccgtgct tgacgacggt ctgccccggc tcggtcagct cgcagttgcc
     4621 gcccgtttcc ccggcaagat cgacgaccac actgccgggc ttcatgccct ccaccgccgt
     4681 ggcggtcacc aaccgcggtg ccgggcggcc cggaaccaac gcggtcgtga tcaccacatc
     4741 gaagccggtg atcgcttcct caagtttctt ctgctgctcg gctttttcct cgtcggacag
     4801 ctcgcgggcg tacccgccct cgccggcggc ctcgatgccc aagtccagcc attgggcacc
     4861 caccgagcgc acctgatcgg ccacctcggg acgcacgtcg tagccggtgg tcttgccacc
     4921 gagccgcttg gccgtcgcca gcgcctgcag ccccgcaaca cccacgccga gcaccagcac
     4981 gctggccggc ttcaccgtgc cagcagcggt ggtcaacatc gggaagaacc gcgtcgactc
     5041 cgacgccgcc accaacaccg ccttatatcc cgcgacgttg gcctgtgacg acaacgcgtc
     5101 catcacctgc gcacgcgaga tccgcggaat ggcctccagt gcgaacgcct gcactccggc
     5161 gctcttcagc gcgtcgatct gattgtcggc gttgcgcggc gccagaaacc cgatcagcgt
     5221 ctgtccgctc ttgagtttgc cgacctcgtc ggccgtcggc ggcgcgacct tgaccaccac
     5281 gtcggcccgc catgcgtcgc cgatcgtcgc gcccgcctcg atatagaggt catcgggcag
     5341 cagcgccgca tccccggcgc ccgactccac gaccaccggt aaacccttgt tgaccagtga
     5401 cgcgaccgcc ttcgggacca gcgccacccg gcgctcgtcg gagcccgact cggcgaccac
     5461 cccgaccgtc gtctgctcag tcatgaggtg caccttaacc gctactgctg gtcacatcta
     5521 aatcggcaca ggttacagtg ctgcgcatgg cggtcgaccg gctgttgccg tcgcaggatg
     5581 cggaagatct catcgcgctg acccgcgata tcgccgacaa ggtgctcgac ccgatcgtgg
     5641 atgcgcacga gaaggccgag acctatccgg agggtgtgtt cggccaattg ggtgcggcgg
     5701 ggctactcag cttgccgcag cccgaggagt ggggtggcgg cggccagccc tacgaggttt
     5761 atctgcaggt gctcgaggag atcgcggcgc gatgggcggc agtggcggtg gcggtcagtg
     5821 tgcacagcct gtcgtcgcat ccgctgctgg cgttcgggac cgacgaacag aagcagcgct
     5881 ggctgcccgg aatgctgagc ggtgagcaga tcggcgcgta cagcctgtcc gagccgcagg
     5941 ccggatccga cgcggcggcg ctgcaatgta aggcaacgaa gtccgcggac ggctatgtgc
     6001 tgaacgggtc gaaatcgtgg atcacccatg gcgggcgggc tgacttctac acgttgttcg
     6061 cgcgcaccgg tgaaggttcg cgtggggtgt cgtgcttcct ggtgccgggc gaccttgccg
     6121 gcctgagctt cggcaagcca gaagaaaaga tggggctgca cgcggtaccg acgacgtcgg
     6181 cgttctacga ccatgcgcct atcgaggccg accggctgat cggcgcgaag ggccagggcc
     6241 tgcagatcgc gttctctgcc ctggattccg ggcggcttgg tatcgccgcc gtcgccgtcg
     6301 ggctggcgca ggccgccctc gatgaggccg tcaagtatgc gaatgagcga acgacgttcg
     6361 gccgcaagat cattgaccat caggggctgg gcttcgtgtt ggccgacatg gccgcggcgg
     6421 tcgccagcgc aagagctacc tatctggacg ccgcgcgcag acgcgatctc ggccggccgt
     6481 attcacagca ggccagcgtg gccaagctgg tggccaccga cgccgcgatg aaggtgacga
     6541 ccgacgcggt gcaggtcttc ggcggtgtcg gctacacccg cgactaccga gtcgagcgct
     6601 acatgcgcga ggccaagatc acccaaatct tcgagggcac caaccagatt caacggctgg
     6661 tcatcgcgcg ggggctgacg acctaaattc ctaccgtggg cggtagacga cggttttgcc
     6721 cgggcgtata ccaggctcat gactatcgag acggcaagca ctcgcatctc gtcgtggcag
     6781 cggaccttga ccgcgtcgag ggtgacaacc gccgaaggcc tgccgcccgg cccccggctt
     6841 ccttcagcgc tgcagtcagt gctgatctgg acgcagcggc agcggttcat ccccgcgatg
     6901 caccgccgct acggcgacac gttcaccgtc aacgccgagc cgacgggatt cggcgttgtg
     6961 ctccgtgatc ctgacgacct gcggaccatc tttcgcgggg atccggagat cttccacgcc
     7021 ggcgaaggca acgcgttgct ggcgccgctg gtcggaaggc gctcggtgct gacgatggac
     7081 gcgcccgacc acatcgagga gcgccggcgc atgctgccgc cgttccacgg tgaacggatc
     7141 gcccgcgtgg tcgccctcat ggaggaggag accgagcgtg agatcgcgca ctggccgatt
     7201 gaccaagatt tcgccctgct tccccggatg caggcgctga ccttgagcat catcgtccgg
     7261 gtggtgctcg gggtggacga ggtcgaccgc gccgaagccg tcggagcggc gttgcgtcgc
     7321 gtattggaga tccggttgcg cgacatgctg ctctgggtgt tgcccagcct gcagcgcctg
     7381 cggccatgga gcaatatcgt gcgcgacctc gaccgggcgg acgagctgat ctacgccgag
     7441 atcgcgcgcc gccgagcgga tcctgcgcga gccgagcggc ccgacgtgtt gtcgatgctg
     7501 ctcgatggtg atcccgatcc ggagctggtg cgcgacgaac tggtcacctt gctggtcgcc
     7561 gggcacgaga ccacagcggt cgcgctgtcg tggacattcg agcggctgct gcgccattcc
     7621 ggcgcgctga gcaaggttca ggcggggctc gacgaccccc gcgatccata ccgggccgcg
     7681 gtggccaagg aagcgttgcg attgcgcccg gtgatcatga acatcgcacg ccgcctgacc
     7741 cagccggtcg agttggcggg ccacatgatg ccggccggca ccctgctgct gccgtcgatc
     7801 atcggcatcc acaccgaccc gaagatctgg ggtcctgacg ccggctattt ccgtcccgaa
     7861 cgctggctgt ccggcgatca accgacctac gcctggattc cgttcggcgg cggcgcccgt
     7921 cgttgcctcg gtgcgacatt cgccctgacc gaaatcgacg ccgtgctgcg gactgtgttg
     7981 cggcgcaacg aattacgcgc cgaccgaccg caggatgaaa agcctcgtat gcaccacatc
     8041 acgctggtgc cgtcgcgcgg cgcccgggtg cgcgtcaccg cgaagactta atcgcgcgag
     8101 cagacggaaa agtgcccttt tacggcgtgt cgcgggactt ttacgtctgc tcgcgaagag
     8161 aatgctccgg cggcgacgtc gccgtcacct gagtcccgta acccacccgc gagtcgatct
     8221 ccatcgagcc gcccatcgcg tcgaaccgtg ccagcagcga gccgagcccg atgtggccgt
     8281 ccgcgacgta ctgcgccacg atcgcgggat cgaagccggt gccgtcatcg tcgattgtca
     8341 acacgacgcg gttgtcctta cgcatcaggc cgaccctgac cgtggtggcc ttggcgtgct
     8401 tgccgatgtt ggtcagcagt tcgcgtgccg cccggtacag cagtgcctgc gactgcggct
     8461 tccccacatc ctccaggtcg gcttcgaccg cgacgtcggt gcgggattcg aactgccgca
     8521 atagttctcg tactgccggg gtgaggccga gttgggccag cacctgaggg tgcagctcgg
     8581 taacggtcga gcgcaatccg gttgcggtgt cctgcaacgc cgcgtagacc atgtccagcg
     8641 cggggtccgg attgcgttcc cgtgcctcgt ccagttcgag ccgtgcggcc agcagcgtct
     8701 gcagcggacc gtcatgcagg tgctcggcca cctcgcggtt gtgccgctcg tcggattgca
     8761 ttgcctccga gaccagttgg cgccgcatct cctgcagcgc cttcacccgc gcggcccggc
     8821 gtgcaagcac gtagcacagc gcggtcacgg cgacggccaa ccagagcagg aagccgaagt
     8881 gcgtgtagac catgttcggc aatccgacct tgtcgtcgcg tttggaatag aagatccaca
     8941 ccgcgagata gcccagcgcc gtgatggttc cgatgatcgc tgtcatcatc ggccggtcct
     9001 ggaacgccac ggagatgggc agcaggaaga agaccggcag cagcgccgcg gtcgcgccgc
     9061 ccgataccaa gcacagcgtg atgatcaccg agaggtcgac ggtcgtcgac gcccaatccc
     9121 cccaccgcgg caccggtccc cgcagcacgg ccaccagcca gagcgccgcc gcagccgcgt
     9181 acacaccaag gatcacggcg tacagcccgg gcagccagtg gtcgacctcc cagatccata
     9241 ccaggacggc gatcaagccg atcagcggca gccgcagaat cgccgagaca cgtatcggtt
     9301 cggcggcgaa gtagtcgacg aattgttgga cggggcgcgc cacgctactc cagaagcttg
     9361 cggcgcatcg cctcggcgac ggcggcggcc cgatcgctga cgccaagttt ctcgtacagc
     9421 cgctgcacgt gggtcttcac cgtcgacggt gccaggaaca gttccttggc catcgcgggg
     9481 atgctgctgc ccgcggcgat cagcttcagc acctcgcgct cccgcgggct cagcactggg
     9541 ccttctggtt cggctcgctt gcggatttcg ccggcgaggc ccgcggccag cgttggtgcg
     9601 acgacgtcgc ggcccttcgc gcagtccagg accgcattga caagttcgct tctggtcgac
     9661 tccttcggga ggaagccggc tgccccctgc tgcagcgcgc cgaagacgat cgccgactcg
     9721 tcgtgcgccg acaccagcag caccctggtg ggcaactcgt cgcggagcac agctgccgcg
     9781 acctcggcac cgtccatacc cggcatccgg taatccagca gcgccacctg cggcgaatgc
     9841 gtcctgatcg cttccagggc cgatggcccg tcgtcggctt cggccaccac ctcgatggac
     9901 ccactcgacg tcagcgcccg gaccacacca tcgcgaaaca gtggatggtc atcacccacc
     9961 accacccgca ccttttcgct ggtcatgcag ccagcgttgc acagcacccg cctgttcttc
    10021 ctccgattgg gggacacgtg attcatccgt gtcgccgtct gattgccgga catacaaggc
    10081 gcctttagac cggcactctt aagtcatcgc cggaacacac cggcgcacaa caccaaaagt
    10141 caacgcagac aggaaaactc acatgaacac caaccgcttc gcacgcatca tcgccctacc
    10201 gatcgcctcg gccggaatca tcggcggcgc cgccctcggc ctggccggtg cggccagcgc
    10261 agccaccacc gacgtccagc agcccaacat cgtcgcggtg ccgtcggtca aggcgcaccc
    10321 ggcgccgcag gcccagcccg gctggtactg gcaccacggc gtgcaccacc tcgccgagct
    10381 gcagccgagc tacacccgct gataacccca tagaccgaag ccctccccta agtagccctg
    10441 gggagggctt ctctatgcgc ggccggccgc atatcccccg atcggtggac acgggattcc
    10501 acccattcaa ccgccgatca cccgacagac ctagccgacc aaacgcgaga gagttatctc
    10561 aacgccggag agaccggcag gagaaccgaa aagcccatcc ccacaggaga actcagatga
    10621 ccatcacccg cagcttcgcc cgctacatcg ccctgcccgc catctcggca ggcatcctcg
    10681 gtggcgccgc cctcggcctg gcaggcatgg ccaatgcagg cacctactcc taccagccga
    10741 ccccgcgccc aggcatcgtc gccaccccga acacgatcgc ccggccgccg gtcatcgtga
    10801 tcccccgcca ccggcaccac gacggcggtc tgatcatcaa cctcccggcc ggcgaatagc
    10861 cagaccactc tcctgaggcc cttcccgaca acctccccgg ggagggcctc ttcacgtcac
    10921 cacaaaggaa tgtcgagtcc atactccgac tccgggcgag ggccgaagta gcgccgctcc
    10981 gattcgtcga tcttcacgtc attgatgctg gcttcacggc gtgccatcag gccttctggc
    11041 gtgaattccc acagctcgtt gccgtagctg cggtaccact gcccgtcggc gtcatgggat
    11101 tcgtattgaa aacgcaccgc catccggttc tcccgaaagc cccacaggct cttgcgcagg
    11161 acgtagtcga gttcgcgctg ccacttgcga gtgagaaagg cgacgatctg ttctcgcccg
    11221 acgacgtgct cgtcgcggtt ccgccactgg gagtcgacgg tgtacgccag gctgacccgt
    11281 tcggggtcgc gggtgttcca ggcgtcctcg gcggcctgga ccttctgcct cgcggtctcg
    11341 agggtgaacg gtgggaacgg cggtcggctt tcggtcatgt catgttctta cgctcgacgg
    11401 actgcggcga ttcccctaat cgaagggtgt catatcgtgc aggcatggac ttcgcgatgt
    11461 cggccaaggc gcaggactac cacaagcggc tgaccgcttt catgaccgag ttcgtcttcc
    11521 ccgccgagga ggcgtacgac cggtatcgcc gggaagctgg ccccgaggac cacacggtgc
    11581 cgccggtcgt cgaagaactc aagaagctgg ccaaggaacg cgggctgtgg aacctgtttc
    11641 tgcccgccga gtcgggactg acgaatctgg agtacgcgcc gctggccgag ctgaccggct
    11701 ggagcctgga gatcgccccc gaggtcacca actgcgcggc cccggacacc ggcaacatgg
    11761 agaccctgca tctgttcgcc accgaagcac agcgcaagca atggttggaa ccgctgctgg
    11821 ccggcgagat ccgcagcggg ttctcgatga ccgagcccgc ggtggcctcc agcgacgccc
    11881 gcaacatcga aacactgatc acccgcgacg gcggcgacta cgtgatcaac ggccgcaagt
    11941 ggtggacgac cggggcgtcg gacccgcgct gcaagatcct gatcgtgatg ggtcgcacga
    12001 accccgacgc ggccagtcat cagcagcagt cgatggtgct ggtgcccgtc gatacccccg
    12061 gggtgaacat tctgcggtcg acgccggtgt tcggctggca ggaccagcac gggcactgcg
    12121 agatcgtcta cgacaacgtg cgggtgccgg tggagaacct gctcggggag gaaggtgcgg
    12181 ggttcgcgat cgcccaagcc cggctcgggc cgggccgcat tcatcactgc atgcgcgcgt
    12241 tgggcgccgc cgagcgagcg ctggcgctga tggtcgaccg ggtgcagaag cgggtggcgt
    12301 tcggcaagcc acttgccgag cagggcgtgg tgcgcgagca gattgcgcgt tcgcgcaacg
    12361 agatcgacca ggcccggctg ctgtgcgaga aggcggcgtg gaccatcgac cagcacggca
    12421 acaaggaggc ccacgtgctg gtctctcaga tcaaggcggt cgccccgcag gtggcctgtg
    12481 atgtcatcga tcgcgcgatc caggtgcacg gcgcggcggg ggtgtccgac gacttcccgc
    12541 tggcccggct gtacagctgg caccgggcga tgcggctgtt cgacggcccc gacgaggtgc
    12601 acatgcgcac catcgcgcgg gcggagatcg gtcgcgagaa gagcgccttc gccgcggcgg
    12661 tgacaagcta gtggcggtgg acgtcgggga actgtcgggg gcgtggaact ttcgtgatgt
    12721 cgccgaggag acaggtgtgc ggccggggcg tttcttccgg tccagcgagc tgagccggct
    12781 cgacgactcg ggccgcgagg tctttcgcca gctggggatc accgatgtcg ccgacctgcg
    12841 atcggcccgc gaagtagagc gtcacgggcc gggtctggtg cccgagggcg tggaagtaca
    12901 ccttttgcac ttccacgagg ttgccgctgt cagcggggag gcgccgcacg aaacggcgtt
    12961 ccagaagatg atgagcgaga agcccgacga cgaagacgtc gcggtggccg cccaccggtt
    13021 catgaccgag gagtacctcc gattcccgac cctcggcggt gcgcaacgtg ctgtgcggca
    13081 agtcatttcg ctgcttgccg atgggcggcc ggtgatcgcc cactgcttcg cgggcaagga
    13141 cagaactggc ttcaccgtgg cgacggtgct cgatgccatc ggtgtggacc gcgacgccat
    13201 catggccgat ttcctgcgaa gcaacgcggc ggtgccacga ctgcgcgaac agatcctgga
    13261 gtcggtgcgc gaccgttccg agactcacga ggtaatcacc ttcgccgagg cgaggctgac
    13321 cgacgaggtg ctgggcgtgc aggaggatta tctagatgcc tctcgtcgct cgatcgagca
    13381 gaactacggc tcgctgaagg actacctcca cgcggcaggt gtggcagcga ccgatattgc
    13441 caaggcgcgc gcgaccctcc tgggatagtt cccgaattgc ccgtgtagca agcgacagct
    13501 cggttgtcgc tcggtacgcg ggcaaaaacg aaagtgccct tccgcggaga tgggtagcgt
    13561 ccttaccgac gatgaatccc gatccagcgc actcgcttgt actgactacc gactaccgcg
    13621 tgcccgatcc gacgcgcgtg tggccgctgc tgcagcgacg ccgcgatgaa cttgccgcgc
    13681 tcggtgcgca ccacgtgttc gtcttcacct cgaccaccga ccccgagcgc gtgctggtga
    13741 cgatcgcact gcgcacccgc gaaccggtcg ccgaactgtt gcggtcccga gcgttcttcg
    13801 agtggttcga cgaagtcggt gtcgacgatc tgcccgccgt ctttgcgggc gagctgatcg
    13861 agcggttcga tttcgacggt gccgacaccg ctccgcccgg catcgtcatt gccggcatca
    13921 tggtcgtcga cgaccccgcc gccttccgtc ttcacgttcg cgagacgcga gacctgttcc
    13981 agaaagcggg aattcgacgg gtgcgaatct tcgaagcctt cgatgatccc gacgaaatca
    14041 tgttcctgca cgagctgtcc gacgaggtaa gcgcgcgacg ctggctgcgc cggccggacg
    14101 tcgccgccga gtggttctcc gacgccggcg tcggcgccta tccgccggtg ttcatcggca
    14161 ggatcgcgca catgatgcgc atcagtgacg agtcctgatg tatgtatgcc tgtgtcttgg
    14221 cgtcaccagc gacaccatca gtcgggccgt cgccgcaggc gcgacgacgt ccaaacaggt
    14281 cgccgacgcg tgcggcgccg gatcggaatg cggccgctgc cgacggaccg tgcgcgcgat
    14341 catcgccgct actgggtcag atagacacca gccacccagg ccgccgtagc gagcagcgcg
    14401 ccacacagtt ccacgcccac cgacagcgcg acacccttga gcgcgtgctt ggtcgacgtc
    14461 cacgccaccc gctggtcccg acgatgcgaa agttccgcca ggtacacgcc gaggacaaag
    14521 ccgatcacca gacccagcac cggaatgacg aaaaagccga taatcccgag caccgcaccg
    14581 gccgccaggc tcatcgtgcg gacgtcggcc tcccgcatcc gccgcatcgg ccacagatat
    14641 ttgatcagcg tcgagacggc caccaccacg gtcaccacgc cgagtgtcac ccaggcggtg
    14701 acgttgtgct cgacgaccgc ccacactgcg atcgcaccga acaccaacaa cgtgcctggc
    14761 agcagcggta ccacgattcc cacgatcccg accgcaatgg ccagcgcgac gagaacgatg
    14821 ccgccggtac tcatgcgccc agctttcccg cgagatcgat gaaatccgtt gcagtgagat
    14881 caaacttgtt ggcgcctacg gtgtggggct cgctgccggg gccatgctcc agtggccggt
    14941 cgacgtacgc tgtccttaac cccgcggcgc tggcggcctt gaggtcgctg gggtgtgcgg
    15001 cgacgagcat gagctcatct ggcgccaccc cgagcaggtc ggcgcagccg aggtaggcct
    15061 cccggtccgg cttgtagtgg tggaacagtt cggcggagat cacgcagtcc cagggcagcc
    15121 cggcgcgctt ggccatgttg gtcagcagcg acatgttgcc gttggaaagc gttgtgatga
    15181 cgaacttttg cttcagccgg gtcagccccg ccacagaatc cggccacggg tcgagtcggt
    15241 gccaggccat gttgagctcg tcgacgtcct cactgcagat cgacgtgacg ccggccgcgc
    15301 ccagcagttc ctcgagaatc aagcggtgca agtcgtcgat tctcgtccac ggaagatcgc
    15361 ccttgcgcac acgatccatc gcgggggcat atccggcgcg ccagtcatcg gcgaagctcg
    15421 gccaatcctg ttgcaggcca tggcgttggc cgaactgctg gagctcacga atgacactgg
    15481 agcgccaatc gacgacggtg ccgaacacgt cgaacgccaa cgccttcgga gtcacggctc
    15541 gagcacgacc ttgccgagca cgccgccgtc ggccagagtc tgtagcgctt ctgcaccctt
    15601 ggccaacggg aaccgcagcg gcggcggcgg acgcagccct gccttgacca attcggcgac
    15661 gccgaactcg aacaccgact gggaccccgg cttgcggttg acgtactcgc cgtatccgac
    15721 gccaatgaca ctgatgttgc gcagcaacag tcgattgact ttgaccgtcg ggatcccgcc
    15781 cgccgcaaag ccgatcacca acagccgacc ctcggtatcc agcgcccgaa cggcgtcgtc
    15841 gaacgcctcg ccgccgat
//
LOCUS       DASRJA010000481         4630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2827471,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000481 DASRJA010000000
VERSION     DASRJA010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2827471"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="VFC01_21105"
     CDS             <1..165
                     /locus_tag="VFC01_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HZN82160.1"
                     /translation="PPPVEDVVGAVHRFAASGCADGTEVVFVQIDGGGHTWPADASQA
                     SAQFFATHGR"
     gene            complement(175..891)
                     /locus_tag="VFC01_21110"
     CDS             complement(175..891)
                     /locus_tag="VFC01_21110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HZN82161.1"
                     /translation="MGLGTAAATLGLPKASAANLRLIDFTERLVPVDQLKSAGYAGAL
                     VYVSELRPGADFDFKPVTREYADALRAAGLHVVSCYQYGKPGWPTPSDFTRGYDGGVA
                     DAQTALRLHAAAGGPDTAPIFFSVDEDIDANAWKSLAAEWFRGINSVLGVDRSGIYGH
                     SRACAWAVADGVVGRSTTPGYRWTWQTSAWSRGEREPTAVLYQSAVNTASNPGPLIGG
                     IHVDVDDVLATDFGQWDLDR"
     gene            968..1399
                     /locus_tag="VFC01_21115"
     CDS             968..1399
                     /locus_tag="VFC01_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN82162.1"
                     /translation="MSEDTAPGITGVHHFSVTVRDIEASAAWYQRVFRADRVPMQFPH
                     YEREDTGYGVLLVDPRSGLAIGLHTNTGNDGEEFDEARTGLDHVGFNVAAREELEAWA
                     AWLDEVGVEHSGVRSGDEPFPFATLVFRDPDNIQLELFTVC"
     gene            complement(1411..1866)
                     /locus_tag="VFC01_21120"
     CDS             complement(1411..1866)
                     /locus_tag="VFC01_21120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82163.1"
                     /translation="MSQTLSAVAVPVDAVRPPVFSRIAARLCARHLDRLVASGVSPQP
                     GTALDVHMLRLCSASHREHLAAELRRRGTVVLCDRVMSISPSRVNVPAVWDAADLIER
                     VEQRLLTHHRVRPRGTARLRRLLADWNGPLRRGGSGDLCSELRAVLAAL"
     gene            complement(1960..2898)
                     /locus_tag="VFC01_21125"
     CDS             complement(1960..2898)
                     /locus_tag="VFC01_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="R2-like ligand-binding oxidase"
                     /protein_id="HZN82164.1"
                     /translation="MNRTRSHSLVAGGLNWDSLPLKLFAGGNAKFWDPADIDFSRDRA
                     DWEALSDLERDWAARLCAEFIAGEEAVTQDIQPFMAAMRAEGRLGDEMYLTQFAFEEA
                     KHTQVFRMWLDAVGMTSDLQRYLDDLPTYRKIFYEELPESLDALTTDPSPAAQVRASA
                     TYNHVIEGMMALTGYYAWHRICVDRGILPGMQELVRRIGDDERRHMAWGTFTCRRHVA
                     ADDRNWAVFETRMNELIPLALQNTDDAYALYDEIPFGLEKDEIQQYAADKGMRRFGTI
                     SSARGRPVEEIDLDYSPLQLEDTFAAEDRQALAALA"
     gene            complement(2895..3584)
                     /locus_tag="VFC01_21130"
     CDS             complement(2895..3584)
                     /locus_tag="VFC01_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN82165.1"
                     /translation="MPTVTWARLDPNRRAAVVEAAEAEFGAHGFSQGSLNVIARRAGV
                     AKGSLFQYFADKRDLYAFIADVASQRVRAYMEDRIRELDPSRPFFEFLTDLLDDWVAY
                     FADHPHERSLHAAASLEVDTDARISVQTVVHRHYLEVLRPLVHDARTRGDLRADSDTD
                     ALLSLLLLIVPHLALAPYVRGLDPILGLDEPTPEQPALAVRRLVAVLKDAFAEPSRMT
                     PTTARSQEVIP"
     gene            3974..4108
                     /locus_tag="VFC01_21135"
     CDS             3974..4108
                     /locus_tag="VFC01_21135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82166.1"
                     /translation="MDDPAGIGAHTHLLEAGQWPVVISLQRTGSAGLLGEITTGPLRS
                     "
     gene            complement(4445..>4630)
                     /locus_tag="VFC01_21140"
     CDS             complement(4445..>4630)
                     /locus_tag="VFC01_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82167.1"
                     /translation="RVYLEEADNAPRAAARLYTHRNTILKRIARATELLGYPPAERRL
                     AVELALELRRHLPPPAS"
BASE COUNT          793 a         1428 c         1565 g          844 t
ORIGIN      
        1 ccgcctcccg tcgaggatgt cgtaggcgct gtgcaccggt tcgccgcatc aggttgcgcc
       61 gacggtaccg aggtcgtctt cgtccagatc gacggcggcg gtcacacgtg gcccgccgac
      121 gcctcgcaag ctagcgcgca gttcttcgcc acgcacggca ggtgagcgag acgatcatcg
      181 gtcgagatcc cactgcccga aatcggtcgc gagaacgtcg tcgacgtcaa cgtgtatgcc
      241 gccgatgagg gggccggggt tagaggcggt gttgaccgca ctctggtaca ggaccgccgt
      301 gggctcgcgt tcaccgcgcg accacgcgct cgtctgccat gtccaccggt acccgggagt
      361 ggttgagcgt ccgacgacgc cgtctgcgac tgcccacgca cacgctcgcg aatggccgta
      421 gatgccggag cggtcgacgc ccaacactga gttgattcct cgaaaccatt cggccgcaag
      481 gcttttccac gcgttggcgt cgatgtcttc atcgacactg aagaagatcg gcgcggtgtc
      541 cgggcctccg gctgccgcgt gcagtcggag cgctgtctgg gcgtctgcca cgccgccgtc
      601 gtaaccgcgg gtgaagtctg acggcgtggg ccaaccgggt ttgccgtact gatagcagct
      661 gacgacgtga agacccgccg cgcgtagcgc atccgcatac tcgcgtgtga cgggtttgaa
      721 gtcgaagtcc gcccctggcc gcaattcgga tacatagacg agcgccccgg cgtagcccgc
      781 cgacttgagc tgatccaccg ggaccaaccg ctcggtgaag tcgatcaacc tcaagttggc
      841 ggccgatgcc ttcggtaggc cgagcgtcgc ggccgccgtc cccaggccca ataggacgag
      901 gacgtcgcgt ctgcaaacag accgcacgcg gcgatgctaa caacatccga ggcggtaggt
      961 tctgggcatg agcgaggaca ccgccccagg cattacggga gttcaccact tctcggtgac
     1021 tgtgcgcgat atcgaagcca gcgccgcctg gtaccagcgg gtgtttcgtg cggaccgggt
     1081 gccgatgcag tttccgcact acgaacgcga ggacaccggc tacggcgtgc tgctcgtcga
     1141 tccgcgttcg ggtctggcga tcggattgca cacgaatacc ggcaacgacg gcgaagaatt
     1201 cgacgaggcc cgaacgggat tggaccacgt cggattcaac gtggccgcac gtgaggagct
     1261 ggaggcgtgg gccgcgtggc tcgacgaggt cggcgtcgag cactccggcg tccgatccgg
     1321 cgacgagccg ttcccattcg ccacattggt gtttcgcgac cccgacaaca ttcagctcga
     1381 actcttcacg gtctgctgac actgcgcgtt ctacagcgca gccagcaccg cgcgtagttc
     1441 gctgcacaga tcgccgctgc caccgcggcg cagcgggcca ttccagtcgg ccagaagacg
     1501 gcggagccgc gcggtgcctc gcggcctgac gcgatggtgc gtgagcagac gttgttcgac
     1561 ccgctcgatc aggtcggccg cgtcccacac tgcgggcacg ttcactcggg acggcgatat
     1621 cgacatgacg cggtcgcaca gcacgacggt gccgcggcga cgcaactcgg cggcaaggtg
     1681 ttcccggtgc gaggcagagc acaatcgcag catgtgcacg tcgagcgcgg tgccaggttg
     1741 cgggctgacg cccgacgcga ccagtcggtc caggtggcgc gcacacaacc gagcggcgat
     1801 gcgcgagaag accggtgggc gcaccgcgtc gacagggacc gcgaccgcgc tcagtgtttg
     1861 ggacatgtga cgagtatgtc ctcgccgcag tcggcagcga ttcgggcgat ctgccggttc
     1921 accgggggaa ttcggtgtcc cccgatcggg ggatagtggc tatgccaagg cggccagcgc
     1981 ttgtcggtcc tcggcggcga acgtgtcttc tagctgcagc ggcgaatagt cgaggtcgat
     2041 ctcctcgacc gggcgtcccc gcgcgctgct gatcgttccg aatcgccgca tccccttgtc
     2101 ggcggcgtac tgctgaatct catccttctc caggccgaac gggatctcgt cgtaaagggc
     2161 ataggcgtcg tcggtgttct gcaatgccag cggaatgagc tcgttcatgc gtgtttcgaa
     2221 gacggcccaa ttgcgatcgt cggcggcgac atggcgccga caggtgaacg tgccccacgc
     2281 catatgccgt cgttcgtcgt cgccgatgcg cctgaccagt tcctgcatgc cgggcagaat
     2341 gccgcggtca acgcaaatcc ggtgccacgc atagtatccc gtcaacgcca tcatgccctc
     2401 gatgacgtgg ttgtaggttg ccgacgcccg aacctgggcg gccggagagg gatccgtcgt
     2461 caaggcgtcg agtgattcgg ggagttcttc gtagaagatt tttcggtacg tgggaaggtc
     2521 gtccaggtag cgctgtagat cgcttgtcat cccgacagcg tcgagccaca tgcggaacac
     2581 ctgggtgtgt ttggcttctt cgaacgcgaa ctgcgtcaag tacatctcgt cgccgagtcg
     2641 cccttcggcc cgcatggccg ccatgaacgg ctggatatcc tgggtgaccg cttcttcgcc
     2701 ggcgatgaac tccgcgcaca accgggccgc ccagtcgcgc tcgagatccg acagcgcctc
     2761 ccagtcggct cggtcgcggg agaagtcgat gtcggcgggg tcccagaact tggcgttgcc
     2821 tccggcaaac agcttgagcg gcaggctatc ccagttgagg ccgccggcga caagtgagtg
     2881 tgatcgtgtc ctgttcatgg aatgacctcc tgagatcttg cggtggttgg tgtcattcgc
     2941 gacggctcgg cgaacgcgtc cttcaggacg gcgacgagac ggcgcacggc cagtgccggc
     3001 tgttccggcg tgggttcgtc aagcccgaga atcgggtcca gcccgcgcac gtagggcgcc
     3061 agcgccagat gcggaacgat cagcagcagc agcgacaaca atgcgtcggt atcggaatcg
     3121 gcgcggagat caccacgcgt ccgtgcatcg tggacaagcg gccgcaggac ttccaggtag
     3181 tggcggtgaa cgacggtctg cacgctgatg cgggcgtcgg tgtcgacctc caggctcgcc
     3241 gcggcatgca gcgaacgttc gtgcgggtga tcggcgaagt acgcgaccca gtcgtcgagc
     3301 agatcggtga gaaattcgaa gaacggcctg cttggatcca gctcacggat gcggtcttcc
     3361 atgtacgcac gcacgcgctg gctggcgaca tcggcgatga acgcgtaaag gtcgcgctta
     3421 tccgcgaagt actggaacag gctgcccttg gccaccccgg cccgccgggc gatgacgttg
     3481 aggctgccct gtgagaaccc gtgcgcgccg aactcggcct cggcggcctc gaccaccgcg
     3541 gcacgacgat tcggatccag gcgagcccac gtcaccgtcg gcataggcac ctccttgcgc
     3601 aatatgacca ctggtcatat tactgtgagg cgcgtcacag tcaaggccgc cgacggctac
     3661 tgtcgagatg caatgaagat tagcgagtag ggcattcttg tttaactagc cggcccgccg
     3721 gtctcggtaa ctggtctagc agcggtttcg atcactgggg gtatgggcgc gtaccagaat
     3781 tcggacgcgt gggcgccttg gctgtgtctt tttgcgctgg cctgcagatt cgtcgttttg
     3841 tagttatgtc aggtatatcg tcgattgtgg cgttgtggcg gcacccttgt tgttagcgtc
     3901 ttcacatgtc catcgccggt tgtcggcgcg tgcgtggcca ctaaggcatc ccgcgcagtt
     3961 gtcccgggga gcggtggacg atccggcagg gatcggtgcc catacgcatc tcctcgaggc
     4021 ggggcagtgg ccggtcgtga tctcactgca gcggaccggg tcggcggggc tgctcggcga
     4081 gatcacgaca gggccgctgc gctcctgaca cgtggcgtgg acttagtcag ctgggaattt
     4141 tggttctctg ggtctgccgc ggcggaggct tggaactggc aggtgcaggg cagccctgcg
     4201 gtgcgtcttc ttcaacctgt ccgaacgacg cgtgcctcgc ggcgcgcgag gcacattcct
     4261 gtgacagtcg acggtttcga tcaaagagtt gcatcgccgg attcgagttc ggctcagtga
     4321 taatggtgtc actgacccga gggtgcccga gcggaacacc ggcgagtatg tgtcgacatt
     4381 gaaaaaacaa cggggcgcga cccgcgcgca gcgtagtcga agtctaggaa aacatcaggg
     4441 gcattcagct agccggcggc gggaggtgcc gtcgaagctc cagggctagc tcgacagcga
     4501 gccgtcgctc ggcgggcgga taaccgagca gttcagtcgc gcgtgcgatc cgcttgagga
     4561 tggtgttgcg gtgggtatac agccgggccg cggcgcgcgg cgcgttgtcg gcctcctcga
     4621 ggtagacacg
//
LOCUS       DASRJA010000482         7966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4043274,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000482 DASRJA010000000
VERSION     DASRJA010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4043274"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VFC01_21145"
     CDS             complement(<1..1380)
                     /locus_tag="VFC01_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HZN82168.1"
                     /translation="MGASLLAVLVVSVLLAAVARHYDVSAPLALVVAGLLAGLIPGFK
                     QIELDPELVLYVLLPPLLWSAGLESSYVALRRNIRPIGLLAVGLPLATTFAVGFVAYK
                     TVPELTVAAALTLGAIVAPPDAVSATAVGRKLGLPRRVMTLLGGESLLNDATALTAYK
                     VSLAAAIGTAATWRSGLATFALAAAGGVVVGFALGMITAFIRWRLDDPLVESALGLVA
                     PFIIYLAAEEIHGSGVLAVVVAALLLGQKSTRAGYATRLQDNAVWKALTLILESFAFL
                     MIGLQLPTVVGELEGITAKVIAMSSVAVLATVILVRIVWVFVFAYLPRALFPRIRDRE
                     PAPTPAQVFVVAWAGMRGVVSLAAAFGVPLTTMSGAPFPGRPQLVFLTFVVVIGTLLL
                     HGLTLPWLIRRLGVQGDEARTDAIAAAAAQDKAARAAAERLDELLAEEEVTDVHERAA
                     DVLRGWNTRR"
     gene            1405..1722
                     /locus_tag="VFC01_21150"
     CDS             1405..1722
                     /locus_tag="VFC01_21150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HZN82169.1"
                     /translation="MLRRSRRSDADAPAVADTCEHLAADAQSPVEPEPLTPGKCQECD
                     ELGESTWAHLRMCLTCGHVGCCDSSPHQHASEHFHATGHPVMRSAEPGEDWRWCYIDV
                     RLG"
     gene            1828..2988
                     /gene="purT"
                     /locus_tag="VFC01_21155"
     CDS             1828..2988
                     /gene="purT"
                     /locus_tag="VFC01_21155"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HZN82170.1"
                     /translation="MLLGSGELSRELALAFQRLGADVIAVDRYADAPAHGVADRAAVI
                     KMNDAEELTALIEKENPRYVVAEAGVIAADALIAVAERGNIEVFPTPRSTRLSIDREG
                     LRRLASDELGLPTAPFWFAGSVEELTAVAEHAGFPLVVKPIAAAPGDGQSVLVRADDV
                     EPAWHRAIAAGRIPHSRVMAETVVDVDFEVTLLTVRTTGPSGPAVHFCEPIGHRLADG
                     DVLESWQPQQMSPVALDAAKSIAARIVNSLGGRGVFGVELLVRGDEVYFADVRPRPYD
                     SGLVTLRSQRLSQFELHARAILGLTVDTIMISPAAAEVLYAGAESTGNHDVDLRLVGE
                     ALAVAESDVRLFDRPDETAGRRRLGVALATAPDPIVARDRARRVSAALRKLW"
     gene            3024..3431
                     /locus_tag="VFC01_21160"
     CDS             3024..3431
                     /locus_tag="VFC01_21160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lumazine-binding protein"
                     /protein_id="HZN82171.1"
                     /translation="MAPFLAALTIIVLVVIAIGLFNIFGDNDPPEDQQVGRAAVGQND
                     ALQRQNYADFRSYTCRDQQGVEADVLARQRDSVAKRGERFVDDVTDVKIDGDRATAKV
                     TYHFDKAPDTKTGVAMTFVREDGAWKVCSTGPN"
     gene            3451..3864
                     /locus_tag="VFC01_21165"
     CDS             3451..3864
                     /locus_tag="VFC01_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZN82172.1"
                     /translation="MSYAGDITPEEAWKLLSDDPEAVLVDCRTDAEWRFVGVPDLSSL
                     QRDVVYVEWNTTDGKHNDNFVDDLKSAGVTPGERPVVFLCRSGNRSIGAAEAATDAGI
                     APSYNVLDGFEGNLNEQRHRGGTGWKAVGLPWKQS"
     gene            3938..5155
                     /locus_tag="VFC01_21170"
     CDS             3938..5155
                     /locus_tag="VFC01_21170"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HZN82173.1"
                     /translation="MSSDEVPSVRKPAELPDGVSQATIGVRGGLLRSGFEETAEAMYL
                     TSGYVYSSAAEAEKAFTGDIDRYVYSRYGNPTISMFEERLRLIEGAPACFATATGMAA
                     VFTSLGALLAAGDRLVAARSLFGSCFVVCSEILPRWGIETVFVDGDELSQWEEALSVP
                     TQAVFFETPSNPMQSLVDIAAVCDLAHAAGAKVVLDNVFATPILQQGFPLGVDVVVYS
                     GTKHIDGQGRVLGGAILGDKQYIDEPVQKLMRHTGPALSPFNAWTLLKGLETLAVRVE
                     HQNTAAQRIAEFLEKHPAVSWVRYPFLESHPQYDLAKRQMTGGGTVVTFELKGGTKDR
                     AFEVLDKLRIVDISNNLGDSKSLITHPATTTHRAMGPEGRAAIGLGDGVVRISVGLEG
                     TEDLIGDLDQALS"
     gene            5156..5566
                     /locus_tag="VFC01_21175"
     CDS             5156..5566
                     /locus_tag="VFC01_21175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82174.1"
                     /translation="MSRRTEAKKNRRNKRRATRDANWVPQEVLDDLTDEVELADVLEA
                     FDELVTQRGWTFDEENSDERGVAWVYEPSSDNSDDYEGHPTTIWIHETEPDWIYLVFI
                     GSREGYQFEPDAILEHLDAIEAYRMGDPLPEFDS"
     gene            complement(5563..6426)
                     /locus_tag="VFC01_21180"
     CDS             complement(5563..6426)
                     /locus_tag="VFC01_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN82175.1"
                     /translation="MDNIRGKTIAITGAARGIGYATAKALLARGARVVIGDRDVALQE
                     SAVADLTKLGPVSGYPLDVTDRESFATFLDKARTDGGGHIDVLINNAGVMPIGPFLDQ
                     SEQAIRSAIEVNLYGVLAGCQLALPDMVARRSGHIINIASLSGLIPVPGQVVYVGAKF
                     GVVGLSAALADEMAPHGVEVSVVMPPFTNTELISGTKSSGAIKPVEPEDIAAAVIKTL
                     EKPKTHVAVPTPLRFTAQAAQMLGPRGRRWLNKRLGLDSVFLDFDVNARQSYEQRAQA
                     AQGVAEGSDKD"
     gene            complement(6447..7304)
                     /locus_tag="VFC01_21185"
                     /pseudo
     CDS             complement(6447..7304)
                     /locus_tag="VFC01_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471662.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            7414..7908
                     /locus_tag="VFC01_21190"
     CDS             7414..7908
                     /locus_tag="VFC01_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN82176.1"
                     /translation="MTVPAVNQLRFVAVSQDDPLAAPLLAELAVEYATRYGATQDAVS
                     KWLRGYPADEFAPPGGGLLIGLLADQPVTGGAFRRFDDSTAELKRIWTDSRYRRQGHA
                     KALLAELEAEIVARGYRSVYLTTGDRQPEAEALYLSTGYTRLIEPLPAEGEVYPIAFQ
                     KVLP"
BASE COUNT         1392 a         2661 c         2652 g         1261 t
ORIGIN      
        1 cctgcgagtg ttccagcccc gcaacacatc tgcagctcgc tcgtgcacgt cggtgacctc
       61 ttcctcggcc agcagctcgt cgagccgctc ggccgcggcc cgcgccgcct tgtcctgtgc
      121 ggcggccgcg gcaatcgcgt cggtgcgggc ctcgtcgccc tgcacgccga gccgccggat
      181 cagccacggc agcgtcagcc cgtgcaacaa cagcgtgccg atcacgacca cgaaggtaag
      241 gaacaccagc tgcggacgtc ccggaaacgg cgcacccgac atcgtcgtca acggaacacc
      301 gaacgccgcc gccagcgata ccaccccgcg catgcctgcc catgcgacga cgaacacctg
      361 cgcaggcgtc ggcgccggtt cgcgatcacg gatgcgtgga aacagggcac gcggcaggta
      421 tgcgaacacg aacacccaga cgattcggac caggatcacc gtcgccaata ccgcgacgga
      481 cgacatcgcg atcaccttcg cggtgatgcc ctccagctcg cccaccaccg tcggcaactg
      541 caggccgatc atcaggaacg cgaacgactc gagaatcagt gtcagggcct tccacaccgc
      601 gttgtcctgc agccgggtgg cgtatccggc cctggtggat ttctggccga gaagcagcgc
      661 ggcgacgacg accgcgagta cgccggagcc gtgaatctcc tcggcggcga ggtagatgat
      721 gaacggcgcg accaggccca gcgcgctttc taccaaagga tcgtcgagcc gccaccggat
      781 gaacgcggtg atcatgccca gtgcgaaacc caccacgacg ccgccggccg cagccagcgc
      841 gaaggtcgcc agtccgctgc gccaagtcgc ggccgtcccg atagctgcgg ccaacgacac
      901 cttgtacgcg gtcagcgcgg tggcgtcgtt gagcaggctc tcgccgccga gcaacgtcat
      961 cacccggcgc ggcagcccga gcttgcgccc gaccgcggtc gcggacaccg catccggcgg
     1021 agcgacgatc gcgccgagcg tcaacgccgc cgcgaccgtc agctcgggca cggtcttgta
     1081 ggccacgaaa ccgaccgcaa acgtcgtcgc cagcggcagg ccgaccgcca gcagcccgat
     1141 cggccggatg ttgcgccgca gcgccacgta gctgctttcc agcccggccg accagagcag
     1201 cggcggcagc agcacgtaca gcacaagttc ggggtccagc tcgatctgct tgaagccggg
     1261 gatcaggccg gccagcaggc ccgcgaccac cagcgccagc ggggccgaca cgtcgtagtg
     1321 cctggccacc gcggccaaca ggaccgacac caccagtacc gccagcagtg aggcacccac
     1381 accgacctcc tatccttggt catcatgctg aggagatcac gccggagtga cgccgacgcc
     1441 ccagcggtcg ccgatacatg cgaacacctc gctgccgacg cgcaatcccc ggtcgagcct
     1501 gagccgctca cacccggcaa atgccaggag tgcgacgagc tcggtgaatc cacgtgggca
     1561 cacctgcgca tgtgcctgac gtgcggccat gtcgggtgct gcgattccag cccacatcaa
     1621 catgcgagcg aacatttcca tgccaccggc catcccgtga tgcgctccgc cgagccgggg
     1681 gaggactggc ggtggtgcta catcgacgtc cgactgggct gaccatctgg caggctggga
     1741 cgcgcgatga gtgaaacaac ggaagaagcg accggacccg aaccggtcgt cgacgacacc
     1801 tccacggcgc ccacgggttc caccgtgatg ctgcttggct ccggcgaatt gagccgcgaa
     1861 ctggcgctgg cattccagcg gctcggcgcc gacgtcatcg ccgtcgaccg ctatgcggac
     1921 gccccggcgc acggcgtggc cgaccgcgcc gccgtcatca agatgaacga cgccgaggag
     1981 ctgaccgcgc tcatcgagaa ggagaacccg cgctacgtcg tcgcggaggc gggcgtcatc
     2041 gcggccgacg cgctgatcgc cgtcgccgag cgcgggaaca tcgaggtgtt tccgacgccg
     2101 cgcagcacgc ggctgtccat cgaccgggag ggcctgcgcc ggctggcctc cgacgagctc
     2161 ggcctgccca ccgcgccgtt ctggttcgcc ggatcggtgg aggagctgac cgcggtcgct
     2221 gagcacgccg gtttcccctt ggtcgtcaag ccgatcgcgg cggcgccggg tgacggccag
     2281 tcggtgctgg tgcgggccga cgacgtcgaa ccggcctggc atcgcgccat cgccgccggc
     2341 cgcattccgc atagccgggt gatggccgag acggtcgtcg acgtcgactt cgaggtcacg
     2401 ctgctcacgg tccgtacgac cggaccgtcg ggtcccgcag tgcacttctg cgaaccgatc
     2461 ggacaccggc tggccgacgg cgacgtgctc gagtcgtggc agccgcagca gatgtcgccc
     2521 gtcgcgctcg atgcggcgaa gtcgatcgcg gcgcgcatcg tcaactcgct cggcgggcgc
     2581 ggcgtgttcg gggtggagct actggtgcgc ggcgatgagg tgtacttcgc cgacgtccgg
     2641 ccgcggccat acgacagcgg actggtgacg ctgcgctccc aacggctttc gcagttcgag
     2701 ctgcacgccc gggcgatcct cgggctgacc gtggacacca tcatgatctc gcccgccgcg
     2761 gcggaagtcc tctatgcagg ggccgagtcg accggcaatc acgacgtcga cctgcgcttg
     2821 gtcggcgaag cgctggccgt cgccgagagc gatgtgcggc tgttcgaccg gcccgacgag
     2881 acagccggca ggcggcggct cggggtggcg ctggccacag ccccggaccc gatcgtggcg
     2941 cgagaccggg cccggcgggt gtcggcagcc ctgcgcaaac tctggtgacc gaaccttccg
     3001 attccgcggc cgaccggccg acgatggcgc cgttcctcgc cgcgttgaca atcattgtgc
     3061 tcgtcgtgat cgcaatcggg ctgttcaaca tcttcggcga caacgacccg ccggaggatc
     3121 agcaggtcgg ccgggccgcc gtcggccaga acgatgcgct gcaacggcag aactacgccg
     3181 acttccgcag ctacacctgt cgtgaccagc aaggtgtgga ggccgatgtc cttgcccggc
     3241 aacgggattc agttgccaaa cgaggggagc ggttcgtcga cgacgtcact gatgtgaaga
     3301 tcgacggcga ccgcgccacc gccaaggtga cctatcattt cgacaaggcg ccagacacca
     3361 aaaccggtgt cgcaatgacg ttcgtgcgcg aggacggagc atggaaggtc tgttcgaccg
     3421 gtcccaacta gctgtggaag acttccgatc gtgagctacg cgggagacat cacgcctgag
     3481 gaggcttgga agctgctgag cgacgatccc gaggccgtgc tggtcgattg ccgcacggac
     3541 gccgaatggc ggttcgtcgg ggtgccggat ctttcttcgc tgcagcgtga cgtggtgtac
     3601 gtcgagtgga acacgaccga cggaaagcac aacgacaact tcgtcgacga cttgaaatcc
     3661 gcgggtgtaa cgcccggtga gcggccggtg gtgttcctgt gccggtcggg caaccggtcg
     3721 atcggcgcgg ccgaggcggc gaccgacgcc ggtatcgccc cgtcctacaa cgtgctggac
     3781 ggcttcgagg gcaacctcaa cgagcagagg caccgcggcg gcaccggatg gaaagcggtc
     3841 ggcctgccct ggaagcagtc atgagcgccc gaagacgacc gcgagtgagg atcgcagcga
     3901 ggaacgagcg aggaccggag cgagcgggag tcgagtcatg agctcggacg aagtgccatc
     3961 ggtgcgcaag cccgccgagc tacccgacgg ggtcagccag gccaccatcg gcgtgcgcgg
     4021 cggcctgctg cggtcgggtt tcgaggagac cgctgaggcg atgtacctga cgtcgggcta
     4081 cgtctactcg tcggcggccg aggcggagaa ggcgttcacg ggcgacatcg accgctacgt
     4141 gtactcccgg tacggcaacc cgactatctc gatgttcgag gagcggctgc ggctgatcga
     4201 aggcgcgcct gcgtgcttcg cgacagcgac gggcatggcg gcggtgttca cgtcgctagg
     4261 ggcgttattg gccgcgggcg accggttggt ggccgcgcgc agcctgttcg ggtcgtgctt
     4321 cgtggtgtgc agtgagatcc tgccgcgctg gggcattgag acggtcttcg tcgatggcga
     4381 cgagttgtcg cagtgggaag aggcgctgtc tgtacccact caggcggtgt tcttcgaaac
     4441 gccgtccaac ccgatgcagt cgttggtcga tatcgccgcg gtatgtgacc tcgcccatgc
     4501 cgcgggtgca aaagtggtgc tggacaacgt ttttgccacg cctatcctgc agcagggctt
     4561 cccgctgggc gtcgacgtgg tggtgtattc aggcaccaaa cacatcgacg gacagggccg
     4621 tgtgctcggc ggcgcgattc tcggtgacaa gcaatacatc gacgagccgg tgcagaaatt
     4681 gatgcggcac accggtcctg cgctgagccc gttcaacgcg tggacgctgc tcaagggttt
     4741 ggagacgctg gcggtgcggg tcgaacacca gaacacggcg gcgcagcgca tcgcggagtt
     4801 tctggagaag caccccgccg tgagctgggt ccgctatccg ttcctggagt cgcacccgca
     4861 gtacgacttg gccaagcggc agatgacggg cggcggaacg gttgtcacgt tcgagctcaa
     4921 gggcggcacc aaggaccgtg cgttcgaggt gctcgacaaa ttgcggatcg tcgacatctc
     4981 caacaacttg ggcgattcca agtcgctgat cacgcacccg gccacgacga cacaccgggc
     5041 aatgggcccg gaagggcgtg cggcgatcgg gcttggcgac ggagttgtgc gaatctcggt
     5101 ggggctggag ggaactgagg atttgatcgg cgatctcgac caggctttga gctaggtgtc
     5161 gcggcggacc gaggcgaaaa agaaccgccg caacaagcgg cgggcgaccc gggacgcgaa
     5221 ttgggtaccc caagaagttc tcgacgacct gacggatgag gtcgaattag ccgacgtctt
     5281 ggaggcattc gacgagctag taacgcagcg cggttggaca tttgatgagg agaactccga
     5341 cgagcgcggc gtcgcgtggg tgtacgaacc gtcatccgac aacagcgacg attacgaagg
     5401 ccatccaacg acaatctgga ttcatgaaac cgagccggac tggatctatc tggttttcat
     5461 cggctctcgc gagggctacc agttcgagcc cgatgcgatc ttggagcacc tcgacgcgat
     5521 cgaggcctac cgtatgggcg atccgctgcc ggagttcgac agttagtcct tgtcggatcc
     5581 ttcggctaca ccctgtgcgg cctgcgcacg ctgctcatag ctctgtcggg cattgacgtc
     5641 gaagtccagg aacacgctgt cgagccccaa acgcttgttg agccaacgcc ttccgcgcgg
     5701 tcccagcatc tgcgcggcct gggcggtgaa ccgcagcggc gtaggaaccg ccacatgcgt
     5761 cttgggtttc tcgagcgtct tgatcaccgc tgcggcgatg tcttctggct ccaccggctt
     5821 gatcgcaccg ctgctcttgg tgcccgagat gagttcggtg ttggtgaacg gcggcatcac
     5881 gactgacacc tccacaccgt gcggcgccat ctcgtcggcc agcgcggcag acagtcccac
     5941 caccccgaac ttggcgccga catagaccac ctgaccggga accgggatca gaccggacag
     6001 cgacgcaatg ttgatgatgt ggccgctgcg gcgcgcgacc atgtccggca gcgccagctg
     6061 gcacccggcg agcacgccgt acaggttgac ctcgatcgcg gatcggatcg cctgctcgga
     6121 ctggtcgagg aacgggccga tcggcatcac accggcgttg ttgatcagga cgtcgatgtg
     6181 gccgccgccg tcggtgcgcg ccttgtcgag gaacgtcgcg aacgactccc ggtcggtgac
     6241 gtcgagcggg tagcccgaga ccgggcccag cttggtgagg tcggcgacgg ccgattcctg
     6301 cagcgcgacg tcacggtcgc cgatcaccac gcgggcgccg cgagccagca gtgccttggc
     6361 ggtggcgtag ccgatgccgc gggcggcgcc ggtgatcgcg atggtcttgc cccggatgtt
     6421 gtccatgcgg ccaaacttta cacgtgtcaa gtttggcgcg gaagtgtggt ggactccagt
     6481 tcacgcttgc gcttgagcat cgctttcgcg agccgcagcg acagcgccgg cgagagccgc
     6541 tccgggtacc accacgcctt ccaccacgcc ggcacgacga tgatcgcgtc atttcgcaac
     6601 acggcacgaa ggacacgctc ggcgaacttc tcgggtgcca tcggccgcaa ccgctcccag
     6661 gatttgagga ggtcctcacg gctgacaccg ggcacgttga ttcgtccgta ctcgccgcct
     6721 gtgaggatcg gagtgcgtat aacgccggga caaagcgccg agacccgcac gccgtgacgt
     6781 tcggcctcca ctcgcaacga cctcgaaatc gcgacgaccg catgcttggt ggcggtgtag
     6841 ctcgccgggc tcacagtaat ggtcagcccg gccatggagg ccgtgttcac gatgtgaccg
     6901 gagtgctgcc ggatcatgat cggctagacc gcttggatgc cgtgcaccac accacgcagg
     6961 ttcacgtcga agacgtcgtt ccagtcatcg aggccgtatg agtcgacctc gccggcgacc
     7021 ccgatgccgg cgttgttgaa caggtagtcg atccgccccg attgcttcac cacctcggcg
     7081 acggcgcgtt caaccgatgg atagtcacgc acatcgagct cgatcgcgcg cgcctttgcg
     7141 ccaccgccgc tgagctgttg cgccaattcc tccgcccggc cgatctgccg atcggcgatc
     7201 caggcctcgg cgccgccctg cgacagcttg gtcgacaatg cggcgccgat gcctgacgcg
     7261 ccgccggtaa cgatcgcgac ctttcctgac acagttgatg ccattgccga aggataattg
     7321 cctgccgtta cgacctgcgg atatttccga tctccctgat cgaatcggcg gctttggaat
     7381 gcggcccgcc gacgcgcgtt agacatttag gtgatgaccg tgcccgccgt gaaccagctg
     7441 cgctttgtgg cagtcagcca ggacgacccg ctggccgcgc cgctgctggc cgagctcgcc
     7501 gtcgagtacg ccactcgcta cggcgccacg caggacgcgg tatccaaatg gctgcgcggg
     7561 tatccggccg acgagttcgc accgcccggc ggcggtctgc tcatcgggct tctggccgac
     7621 caacccgtca ccggcggcgc gttccgccgc ttcgacgact ccaccgccga gctcaagcgc
     7681 atctggaccg acagccgcta tcgccgccaa ggccacgcca aggcgctgct cgccgagctc
     7741 gaggccgaga tcgtcgcccg cggctaccgc tcggtctatc tgacgacggg cgatcgccag
     7801 cccgaggccg aggcgctgta cctgtccacc ggctacaccc gactgatcga gccgcttccc
     7861 gccgagggcg aggtttaccc gatcgcattc cagaaggtgc tgccatgacc gcacagctgc
     7921 atgtcggcct cgcactcgac ggttacggct ggcacccgca ggcgtg
//
LOCUS       DASRJA010000483         8085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3289007,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000483 DASRJA010000000
VERSION     DASRJA010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3289007"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /gene="ffh"
                     /locus_tag="VFC01_21195"
     CDS             complement(<1..1295)
                     /gene="ffh"
                     /locus_tag="VFC01_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZN82177.1"
                     /translation="MFESLSDRLTGALQGLRGKGRLTDADIDATAREIRLALLEADVS
                     LPVVRAFVARIKDRAKGAEVSGALNPAQQVVKIVNDELIGILGGETRKLAFAKTPPTV
                     IMLAGLQGSGKTTLAGKLAKWLKDQGNTPLLVACDLQRPGAVNQLQIVGERAGVQVFA
                     PHPGVSTEGSEQAGHGDPVAVASGGLAEAKAKHFDVVIVDTAGRLGIDEELMTQAAAI
                     RQVLDPDEVIFVLDAMIGQDAVTTAEAFREGVGFTGVVLTKLDGDARGGAALSVREIT
                     GVPILFASAGEKLEDFDVFHPDRMASRILGMGDLLTLIEQAEQHFDAQKAEEAAAKIG
                     SGELTLEDFLEQMLAIRKMGPIGNLLGMLPGAGQMKDALAAVDDSQLDRVQAIIRGMT
                     PAERADPKIINASRRLRIANGSGVAVSEVNQLVDRFFEAR"
     gene            complement(1306..3813)
                     /locus_tag="VFC01_21200"
     CDS             complement(1306..3813)
                     /locus_tag="VFC01_21200"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HZN82178.1"
                     /translation="MTEQTPEPAAGSPHASSGPTRWEAPAAGSLRPATDLSAASEQLL
                     SGAARQLDSAALRDALLDLHDFWLTTKATEIGVTATSGFAIVATGGLGRGELLPYSDL
                     DLTLLHDNMPHDIVSQVAELLWYPLWDANIRIDHSVRTVPEALKVAGEDISAGLAMLE
                     ARHIAGDSDLSSLLIGGARRQWRTGIASRFDELVEHTEQRWQRSGQIAHRAEPDLKCG
                     RGGLRDVQLLNALAIAQLADVYPSRSLASPTGTLGEAHLALLNVRTELHRVSGRGREL
                     LLAQHADEIGAALHIGDRFDLARMLSDAARTVSYYVDAGIRTAANALPRRGLAAFKRP
                     VRRPLDEGVIEFNGEVILARDARPERDPGLILRVAAASATTGLPIAASTLARLTESAP
                     ELRTPWPRQALKDLLVMLAAGPAAVATIEALDRTGLWGRLFPEWGAVRDLPPRDVVHI
                     WTVDRHLVETVSRASAFTTRVSRPDLLVLGALCHDIGKGRGGDHSVIGAELATQIGTR
                     LGLWPSDVELLSKVVRHHLRLPHTATRRDLQDPKTIAEVVDALDGDPVLLELLSVLAE
                     ADSLATGPGVWGDWKSSLIGDLVRRCRLVMAGEPLPKPDPIDPRYLSLASDDSVHVEM
                     TPGDSPHIYNVTMIAPDRRGLLSKAAGVLALNSLRVHSASVNGHQGSAINTFVVSPHF
                     GAPPAAELLRQQFILALDGELDVLASLDKRDRDAAQHGTTRAGEVPDAVPINHATAPP
                     KILWSDGIEPGELVVQIRSTDRAGLLARLTAVFERDGVDIAWAKVTTLGSSVVDVFDI
                     VVPTLASGDRSIRDELEHDLYAVLPAPAPAKPVSEAS"
     gene            complement(3892..4230)
                     /locus_tag="VFC01_21205"
     CDS             complement(3892..4230)
                     /locus_tag="VFC01_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZN82179.1"
                     /translation="MKLITAIVKPFTLEDVKTGLEQTGILGMTVSEVQGYGRQKGHTE
                     VYRGAEYSVDFVPKVRVEVVVDDSAVDKVVDVIVQAARTGKIGDGKVWVSPVDTVVRV
                     RTGERGADAL"
     gene            complement(4237..5601)
                     /locus_tag="VFC01_21210"
     CDS             complement(4237..5601)
                     /locus_tag="VFC01_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZN82180.1"
                     /translation="MDGFPTLGIPDTGDTAWMLASSALVLLMTPGLAFFYGGMVRSRS
                     VLNMIMMSVSAMGVVTVLWALYGYSMAFGNDVANLFGNPTQYWGLKGLIGGTYLQLDA
                     ATDPAVSIPLAGTIPQTVFVAFQLMFAIITVALVSGAVADRLKFGGWLLFVALWITVV
                     YFPVAHWVFAFDGVTGETGGWIANKLKAIDFAGGTAVHINSGTAGLVLAIILGKRIGW
                     PTSPMRPHNLPFVMLGAGLLWFGWYGFNAGSAVASNGIAGATFITTTIATATAMLGWL
                     ITERIRDGKATSLGAASGIVAGLVAITPSCSSVNVVGALVVGLSAGVLCALAVGLKYK
                     LGFDDSLDVVGVHLVGGLVGTLLVGLVAAPEAPAAVTGLFYGGGFDQLWRQAIGAGAV
                     LLYSAVGTAIVALIVKYTIGLRISEEDESSGIDEAEHAETGYDLAAVSGGGSSILARH
                     GAEG"
     gene            complement(5777..7054)
                     /gene="ftsY"
                     /locus_tag="VFC01_21215"
     CDS             complement(5777..7054)
                     /gene="ftsY"
                     /locus_tag="VFC01_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZN82181.1"
                     /translation="MSEALWIAIAVIAVLVVVALVVGLVRYRRRRISLSSADTATPVD
                     RSGGYTASSGITFTQSAPAEPVERIDTSGLPAVGDDATIPRDAPKRTIADVQLPEAPV
                     APPAPEPEPAAAPVEEIAPVEGRLDRLRGRLAKSQNTLGRGVLGLLGGGDLDEESWEA
                     VEDTLLIADLGPVVTESVVSSLRSRMASSGVRTETEARAVVRDVLIEELQPDLDRSIK
                     ALPHHNKPSVLLVVGVNGTGKTTTVGKLARVLVADGRRVVLGAADTFRAAAAHQLQTW
                     ASRVGAEVVSGVEGADPASVAFDAVDKGIENGADVVVIDTAGRLHTKTGLMDELGKVK
                     RVVSRRAEVDEVLLVLDATIGQNGLPQARVFAEVVDITGVVLTKLDGTAKGGIVFRVQ
                     QELGVPVKLVGLGEGPDDLAPFEPAAFVDALLG"
     gene            7083..>8085
                     /gene="fni"
                     /locus_tag="VFC01_21220"
     CDS             7083..>8085
                     /gene="fni"
                     /locus_tag="VFC01_21220"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HZN82182.1"
                     /translation="MTLDPGGMETRKRRHIDVCLSEAVDYQTVTTGLERYRLPYNALT
                     QTDLGSVDLGVDFLGARLRSPVLIGAMTGGAELSGTINRNLAVAAQQLGVGLMLGSQR
                     IMLDNRSASESFSVRDVAPDVLLIGNIGLAQLNEVMVPDLVKALDHVGANALAVHTNP
                     LQEAMQHEGDTDFSGSVSRLRDIAGSIGYPVMLKEVGHGIGAAAAAELVDCPLAAVDV
                     AGAGGTSWARVEQFVRYGEIRYPALAEWGIPTAEALLEVRHTLPDVSIVASGGIRTGM
                     DAAKALAMGADAVAVARPLLAPAIESPAAVLGWLQSFIDELLVCLHGCGAANLAALRR
                     "
BASE COUNT         1375 a         2717 c         2661 g         1332 t
ORIGIN      
        1 cgcgcctcga agaagcgatc gacgagctgg ttgacctcgg atacggcgac gcccgacccg
       61 ttggcgatgc gcagccgtcg cgacgcgttg atgatcttcg gatcggcccg ctcggcaggt
      121 gtcataccgc ggatgatcgc ctgtacccga tccagctggc tgtcgtcgac ggcggcaagc
      181 gcatccttca tctgcccagc gccgggcagc atgcccagca ggttgccgat cgggcccatc
      241 ttgcggatgg ccagcatctg ctcgaggaag tcctcgaggg tcagctcgcc cgagccgatc
      301 ttggcggcag cttcctcggc cttctgggcg tcgaaatgct gttcggcctg ctcgatcagc
      361 gtgagcaggt cgcccatgcc gaggatgcgg ctggccatcc ggtcggggtg gaagacgtcg
      421 aagtcctcga gcttctcgcc cgcggaggcg aacagaatcg gcacgccggt gatctcccgc
      481 accgacagcg cggcgccgcc gcgggcatca ccgtcgagct tggtcagcac cacgccggtg
      541 aagccgacgc cctcgcggaa cgcttctgcc gtggtgacgg cgtcctggcc gatcatggcg
      601 tcgagcacga agatcacttc gtcggggtcg agcacctggc ggattgcggc ggcctgggtc
      661 atcagctctt cgtcgatgcc gagccgaccc gcggtgtcga cgatgacgac atcgaagtgc
      721 ttggccttgg cctcggccaa gcccccagag gcgaccgcga ccgggtcgcc atggccggcc
      781 tgctcggagc cttccgtcga cacgcctggg tgcggcgcaa acacctgcac cccggcgcgt
      841 tcgccgacga tctgcagctg gttgaccgcg cccggccgtt gcaggtcaca cgccaccaga
      901 agtggcgtat tgccttgatc tttaagccat ttcgcgagct tgccggccag cgtggtctta
      961 ccggaacctt gcagaccggc gagcatgatc accgtcggcg gagtcttggc gaacgccagt
     1021 ttacgggttt caccgccgag gatgccgatc agttcgtcgt tgacgatctt gacgacctgc
     1081 tgggccgggt tgagcgcacc ggagacctcg gcgcccttgg cgcgatcctt gatgcgggcg
     1141 acgaacgcgc gcaccaccgg cagcgagacg tcggcttcaa gcagcgccag ccggatctcg
     1201 cgagcggtcg cgtcgatgtc ggcgtcggtc agtcggccct taccgcgcag cccctgcagg
     1261 gcgccggtca accggtcgga cagcgattca aacacgccga ccagcctagc tggcctctga
     1321 gaccggcttc gcaggcgcgg gcgcaggcag taccgcgtag agatcgtgct cgagttcgtc
     1381 gcggatcgac cggtcacccg aggccagcgt cggaaccaca atgtcgaaca cgtcgacgac
     1441 cgacgatccc agcgtggtca ccttcgccca cgcgatgtcc acgccgtcac gttcgaagac
     1501 cgccgtcagc ctggcgagca ggccggcgcg gtctgtcgac cggatctgca ccacgagttc
     1561 gcccggctcg atgccatcgg accataggat cttcggcggc gccgttgcgt ggttgatcgg
     1621 gactgcgtcc ggcacctcgc cggcacgtgt cgtgccgtgc tgtgccgcat cgcggtcccg
     1681 cttgtccaac gacgccagca catccagttc gccatccagc gcaaggatga actgttgccg
     1741 taacagctcg gcggccggtg gcgcaccgaa atgcggtgac accacgaagg tgttgatggc
     1801 cgaaccctga tgaccgttca ccgaagccga gtgcacccgc agcgaattga gggccagcac
     1861 accagcggcc ttggacagca ggccgcggcg gtccggcgcg atcatcgtca cgttgtagat
     1921 gtgcgggcta tcgccaggag tcatctcgac gtgcacgctg tcatcggacg ccaacgaaag
     1981 atagcgcggg tcaatgggat cgggcttcgg cagcggctcg cccgccatga ccaaccgaca
     2041 gcgccgaacc aggtcgccga tcagcgacga tttccagtcg ccccatacgc cgggaccggt
     2101 ggcaagcgag tccgcctctg ccagcacgga aagcagctcc agcaacaccg ggtccccgtc
     2161 gagcgcgtcg actacctccg caatggtttt cgggtcctgc aggtcgcgcc gcgtcgcggt
     2221 gtgcggcagc cgcaggtgat ggcgcaccac cttcgacagc agttcgacat ccgacggcca
     2281 cagacccagc cgggtgccga tctgggtagc caactcggcg ccgatgacgc tgtgatcacc
     2341 gccgcggccc ttgccgatgt cgtggcacag cgcgccgagc accaacaggt cgggccggga
     2401 tacccgggtg gtgaatgcgc ttgcccgcga gacggtttcg acgagatggc ggtcgacggt
     2461 ccagatgtgc accacgtcgc gcggggggag gtcgcgcact gcgccccatt cggggaacag
     2521 cctgccccac aacccggttc ggtccagcgc ctcgatggtg gccaccgccg ccggaccggc
     2581 ggccagcatc accaacaaat ccttgagcgc ctgccgcggc cacggtgtac gcagctcggg
     2641 cgcgctctcg gtaagccggg ccaacgtcga cgccgcgatc ggcaaccctg tggtcgccga
     2701 ggcggcggct acccgcagga tcagtccggg atcgcgttcc gggcgtgcat cgcgagcgag
     2761 aatcacctcg ccgttgaatt cgatgacgcc ttcgtcgagg gggcggcgca cggggcgttt
     2821 gaaggccgcc aatccgcggc ggggcagcgc gttcgccgcg gtgcgaattc cggcatcgac
     2881 gtagtagcta acggtgcgcg ccgcgtcaga aagcatgcgc gccaagtcga atcggtcacc
     2941 gatgtgcagc gcggcgccga tctcatcggc atgctgagcc agcagcagtt cgcgtccgcg
     3001 gccggacacc cggtgaagtt cggtgcgcac gttgagcagc gccaggtgcg cctcgcccag
     3061 ggtgccggtc ggcgaggcca gcgagcggct cggatagaca tcggccagtt gggcgatcgc
     3121 cagtgcgttg agcagttgca cgtcgcgcag gccgccgcgg ccgcacttga gatccggctc
     3181 agcgcggtgg gcgatctggc cgctgcgctg ccaccgctgc tcggtgtgct cgacgagttc
     3241 gtcgaaacgc gaagcgattc cggtgcgcca ctgccgacgc gctccgccga tcaacagcga
     3301 cgacagatcc gagtcgcccg caatgtgccg cgcctcgagc atggccaggc ccgcggagat
     3361 gtcctcgccc gcgaccttca acgcctccgg aacggtgcgc acgctgtggt cgatgcggat
     3421 gttggcgtcc cacaacgggt accacagcag ttccgcaact tggctgacaa tgtcgtgagg
     3481 catgttgtcg tgcaacagcg tcaggtcgag gtcagagtac ggcagcaact cgccgcgccc
     3541 cagaccaccg gtggcgacga ttgcgaatcc actcgtggcg gtgactccga tctcggtggc
     3601 ctttgtggtg agccagaagt cgtgcaagtc cagcaacgcg tcgcgtagtg cggccgaatc
     3661 cagttgacgt gcggcgcccg agagcagctg ttcggatgcg gccgaaagat cggtggccgg
     3721 gcgtaacgag cccgccgccg gggcctccca tcgggtgggc ccactgcttg catggggaga
     3781 gccggcggcg ggttctggtg tctgctctgt catttcgact cctctcccgg cccttaccta
     3841 taaacgctca cggtgttcgt ccgggagacg acgtcttatt tcagatgtgg ctcaaagggc
     3901 gtcggccccc cgctctccgg tgcgcacccg gaccacggtg tcgacggggc tgacccacac
     3961 ctttccgtcg ccgatcttcc cggtgcgagc ggcctggacg atgacatcca ccaccttgtc
     4021 gacggcggaa tcatcgacca caacctcaac ccgcaccttc ggcacgaagt cgacggagta
     4081 ctcggcgcct cggtacacct cggtgtgacc cttctggcgg ccgtagccct gaacctcgct
     4141 gacggtcatt ccgagaatac ccgtctgttc gaggccggtc ttgacgtctt cgagcgtgaa
     4201 cggcttgacg atcgcagtaa tcagcttcat attcccttat ccctccgcgc cgtgacgggc
     4261 cagtatcgag ctgccgccac cactgaccgc ggcgaggtcg tatccggttt ccgcgtgttc
     4321 ggcctcatca atgccggacg actcgtcctc ctcgctaatg cgcagcccga tggtgtattt
     4381 cacgatcaag gccacgatag cggtaccaac ggcggaatag agcagaaccg caccggcccc
     4441 gatggcctga cgccacagct gatcgaagcc gccgccgtag aacaaaccgg tgaccgccgc
     4501 tggcgcctcc ggcgccgcaa ccaggccgac cagcagggtg ccgaccagac ctccgaccag
     4561 gtgcacgccg acgacgtcga gtgaatcgtc aaagcccagt ttgtatttca ggccgacggc
     4621 cagcgcgcac agcacaccgg cggacaggcc gacgaccaag gcgccgacga cgttcaccga
     4681 cgagcaagac ggggtgatcg cgacgaggcc cgcgacgatg cccgacgcgg cgcccagcga
     4741 ggtggcctta ccgtcgcgga tgcgttcggt gatgagccag cccagcatgg ccgtcgcggt
     4801 cgcgatcgtg gtggtgatga acgtcgcacc cgcgatgccg ttggaggcga ccgcagaacc
     4861 ggcgttgaac ccgtaccagc cgaaccacag caggccggcg cccaacatca cgaacggcag
     4921 gttgtgcggc cgcattgggc tggtcggcca accgatgcgc ttgccgagga tgatcgcaag
     4981 caccagcccc gcggtaccgg agttgatatg caccgcggta ccacctgcga agtcaattgc
     5041 cttgagtttg ttggcaatcc agccgccggt ctcaccggtg acaccgtcga acgcgaacac
     5101 ccagtgtgca accgggaagt acaccaccgt gatccacagt gcgacgaaca gcagccagcc
     5161 gccgaacttc aaccggtcgg cgaccgcgcc cgagaccagg gccaccgtga tgatggcgaa
     5221 catcagctgg aatgcgacga aaaccgtctg ggggatggtt cctgccagcg gaatcgacac
     5281 ggcgggatca gtggcggcgt cgagttgcag gtaggtgccg ccgatcagac ctttcaggcc
     5341 ccagtattgg gtcggattac cgaataggtt ggcgacgtcg ttgccgaagg ccatcgagta
     5401 gccgtacagc gcccacaaca ccgtgacgac acccatggcg ctgacgctca tcatgatcat
     5461 gttgagaacg cttctggaac ggaccatgcc tccgtagaag aaggccagcc cgggcgtcat
     5521 caacaagaca agcgctgagc ttgccagcat ccatgcggta tcaccggtgt ccggtatacc
     5581 gagagtcggg aatccatcca ctcgcagttt cctccttcaa catgccacgg ccgcacagtt
     5641 ttacgaccgt tgacctgttg caagacaatg ctgagtggtt gtttcgtcta cgacgctgcg
     5701 gtgtttcgct cgtgtgaacg gatgggccgt ccacgtttcg tcagtgttac atcaggcgtt
     5761 tcttgacgag cgtttgttac ccgagcagcg catcgacgaa cgctgcgggc tcgaacggag
     5821 cgagatcgtc ggggccttca ccgagaccga cgagtttgac cggaacaccc aactcctgct
     5881 gcacgcggaa gacgatcccg ccctttgccg tgccgtcgag tttggtcagt acgacaccgg
     5941 tgatgtcgac gacttcggca aacacgcggg cctgcggcag cccgttttgc ccgatcgtgg
     6001 cgtcgagcac caggagcacc tcgtcgacct ccgcgcgccg gctcaccacc cgcttgacct
     6061 tgccgagctc gtccatcagc ccagtcttcg tgtgcagccg tccggccgtg tcgatgacaa
     6121 ccacgtcggc accgttctcg atgcccttgt cgacggcgtc gaaggcgacg gatgccgggt
     6181 cggcgccctc gaccccactc acgacctcgg cgcccacccg ggacgcccag gtctgcagct
     6241 ggtgggccgc ggcggcacgg aaggtatcgg cggcaccgag taccacgcgg cggccgtcgg
     6301 ccaccagaac gcgggccagc ttgccgaccg tcgtcgtctt accagtgccg ttgacaccga
     6361 cgaccagcag cacggacggc ttgttgtggt gcggcagcgc cttgatcgag cggtccaggt
     6421 cgggctgcag ctcctcgatg agcacgtcac gcaccacggc tcgggcctcc gtctcggtgc
     6481 gcacgccgct gctggccatg cgtgagcgca gcgatgacac caccgattcg gtgacgacgg
     6541 gccctaggtc agcgatcagc agagtgtcct cgacggcctc ccaggactct tcgtcgaggt
     6601 cgccgccgcc gagcaggccg agcacgccgc ggcccagcgt gttctgcgac ttggccagac
     6661 gcccgcgcag gcggtccagc cggccttcaa ctggtgcgat ctcctcgaca ggagctgcgg
     6721 cgggttcagg ctccggagcg ggcggcgcca ccggagcctc tggcagctga acgtcggcga
     6781 tagtgcgttt cggcgcgtca cgcggaatgg tggcatcatc gccgacggca ggcagcccgc
     6841 tggtatcgat gcgttcgacc ggctcagcag gtgctgactg ggtgaacgtg atccccgagg
     6901 acgcggtata tccgccggat cggtcgacgg gagtggcagt gtctgcagac gacaagctga
     6961 tccgtcgacg ccggtaccgc accagcccga cgaccagcgc aaccacgacc agaacggcaa
     7021 tgaccgcgat tgcgatccac agagcttccg acaccacgcc attgtctcag gggtcatagc
     7081 cggtgacgct cgaccccggc ggcatggaaa cccgcaaacg gcgccacatc gacgtgtgcc
     7141 tgtccgaggc agtcgactat cagaccgtca ccaccgggct ggagcgctac cggctgccgt
     7201 acaacgcgct cacccagacc gatctgggca gcgtcgattt gggcgtcgac ttcctcggcg
     7261 cgcggctgcg ttcgccggtg ctgatcggcg cgatgacggg cggggccgag ctgtcgggca
     7321 cgatcaaccg caatctggcc gtggcggctc agcaactcgg cgtgggcttg atgctgggct
     7381 cgcagcgcat catgcttgac aaccggtcgg cctcggagtc attcagcgtc cgcgatgtgg
     7441 cgcccgacgt gctgctgatc gggaacatcg gactggctca actcaacgag gtcatggttc
     7501 ccgacctcgt caaggcgctc gaccacgtcg gcgccaatgc ccttgcggta cataccaacc
     7561 cgctgcagga ggccatgcag cacgagggcg acaccgactt ctccggctcg gtttcgcggt
     7621 tgcgcgacat cgcagggtcg atcggctatc cggtgatgct gaaagaggtc ggccacggca
     7681 tcggtgcggc tgccgccgcg gagttggtgg actgcccgct cgcggccgta gacgtcgcag
     7741 gcgcgggcgg cacgtcgtgg gcgcgggtcg aacagttcgt gcggtacggc gagattcgct
     7801 accccgcgct ggccgagtgg ggcatcccga ccgcggaggc gctactggag gtgcggcaca
     7861 cgctgcccga cgtgtcgatt gtcgcgtcgg gtggcatccg caccggtatg gacgcggcca
     7921 aggccttggc gatgggcgcc gacgcggtgg cggtggcgcg gccgctgctg gcgccagcga
     7981 tcgaatcgcc tgccgccgtt ctgggttggc tgcagtcgtt catcgacgaa ctgttggtct
     8041 gcctgcacgg ctgcggtgcc gcgaatttgg cggcactgcg gcgtc
//
LOCUS       DASRJA010000484         3823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3067864,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000484 DASRJA010000000
VERSION     DASRJA010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3067864"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VFC01_21225"
     CDS             complement(<1..751)
                     /locus_tag="VFC01_21225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZN82183.1"
                     /translation="MVAGSPLLRPRDADALRAELRRVAEESGMPLTFGGEVHDDTLLL
                     TEFFGARTGGMRGLAVMPSAGLGGATVVSRRPLSVPDYRRASSITHHYDGPVLQEGIK
                     SILAVPVVVDGRSRAVLYGAYRAAAPIGDRTADLMIASARRLSRELSIRDEVDRRLRL
                     RDAHHAGIRGNGAAAEEIREVHAQLRRLAADPSAPPALQAQLRVLAERLARGRSGVPA
                     ATLSARELDVLAQVALGCTNDEAAKRLSLKPE"
     gene            811..2514
                     /locus_tag="VFC01_21230"
     CDS             811..2514
                     /locus_tag="VFC01_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN82184.1"
                     /translation="MTSNTDLYRIARDQLVNVIGDYDKAVDLFSWPQLTGTFNWATDW
                     FDVIARGNDRTALWIVEENGTEHKTSFAEMADVSDRVATWLSRLGVSKGDRVILMLGN
                     QVELWESMLAVAKLGAIIMPTTGALGPADLTDRITRGGAKFVIANDEDAAKFDDVHGE
                     YTRIVVGGALEEGAGWHRYSTAYAVPSAGPFATPTNTDDPMLIYFTSGTTSKPKLVEH
                     SQVSYPVGHMSTMAWIGVRPGDVHLAISSPGWAKHAWSCFFAPWIAEATIFVYNYSRF
                     DAAELLNQLRRARVNTFCAPPTVWRMLIQSDLGSKPEGLREILGAGEPLNPDVIAQVE
                     RAWGLTIRDGFGQTETTLQVGNTPGQPVKPGSMGRPMPGVPVVLVDPVTGEPADEGEI
                     CLDLQRNPVNLMTGYLGDPQRNATVMARGYYHTGDVASRDDEGYITYIGRTDDVFKSS
                     DYKVSPFELESVLIEHPAVVEAAVVPQPDETRLCVPKAYVSLAEGWEPNAETARQIME
                     YARDHLAPYLKVRRVEFYELPKTISGKIRRVELRRREEAAHAEGKSIETEHRYEDVVG
                     R"
     gene            2511..>3823
                     /locus_tag="VFC01_21235"
     CDS             2511..>3823
                     /locus_tag="VFC01_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN82185.1"
                     /translation="MKSYDAGPTDTPILEQTIGENFEETVRRNPGAEALVDVASGRRW
                     TYGELNAEIDLIARGLMSLGIEPGERVGIWAPNCTEWTMVQYATAKVGAILVNINPAY
                     RTHELAYVLNQSGVRTLISATAFKTSDYVGMVEEVRPDTHVLRDVVFLGTDDWTELRS
                     RADCVKPGELRTRMASLDNNEPINIQYTSGTTGFPKGATLSHRNILNNGFFTTELINL
                     RPGDRLCIPVPFYHCFGMVMGNLGCTSHGATMVIPAPGFDPGLTLAAIEKERCTGVYG
                     VPTMFIAMLGHPDFVNRDLSSLRTGIMAGSVCPVEVMKRCVNEMNMTEVAIAYGMTET
                     SPVSCQTHVDDDLERRTATVGRAHPHVDIKIVDPETGDTVERGQPGEFCTRGYSLMLG
                     YWNDNDKTREAIDADGWMHTGDLAVMREDGYCNIVGRIKDMVIRGG"
BASE COUNT          709 a         1255 c         1236 g          623 t
ORIGIN      
        1 tctctggctt cagcgaaaga cgtttggccg cttcgtcatt cgtgcaaccc agtgcgacct
       61 gagcgaggac gtcgagctca cgcgccgaca gcgtagcggc agggactccc gatctgccac
      121 gggccagtcg ttcagcgagt acccgtagct gtgcctgcaa cgcaggcggc gcggacgggt
      181 ccgcggccag ccggcgaagc tgcgcatgga cttcgcggat ttcctcggca gccgccccat
      241 taccgcggat gcccgcgtgg tgcgcgtccc gaagccgcag ccgtcggtcc acctcgtcgc
      301 gaatgctcag ctcgcggctg agtcgccggg cggaggcgat catcagatcc gccgtgcggt
      361 cgccgatcgg cgccgcggcg cggtaagcgc cgtagagcac cgcccgcgac ctgccgtcca
      421 ccacgaccgg gacggccagg atcgacttga tgccctcctg aagcaccggc ccgtcgtagt
      481 ggtgcgtgat cgaggaggcc ctgcggtagt cggggaccga caacgggcgt cgggacacga
      541 cggtggcccc gccgaggcca gcgctgggca tcaccgcgag cccccgcatg ccgccggtgc
      601 gggcgccgaa gaactccgtc agaagcaggg tgtcgtcgtg tacctcgcct ccgaatgtca
      661 gcggcatgcc cgactcttcg gcgacccgtc tcagctcggc acgcagcgca tccgcgtcac
      721 gcggacgcag cagcggcgag ccggcaacca tcggcgaaca ccctctttcg ggggtagcgg
      781 gagactagtg tgacgtagat cctatccgcc atgacgagca acaccgacct gtatcgcatc
      841 gcccgcgatc agcttgtcaa cgtgatcggc gactatgaca aggccgtcga tctgttttca
      901 tggccgcagc tcaccggcac tttcaactgg gccaccgatt ggttcgacgt catcgcccgc
      961 ggcaatgacc gcacagcgtt gtggatcgtc gaggagaacg gcaccgaaca caagaccagc
     1021 ttcgcggaga tggctgatgt atccgaccgt gtcgcgacgt ggctgagccg gctcggcgtc
     1081 agcaagggtg accgggtcat tctgatgctg ggcaaccagg tcgagctgtg ggagtcgatg
     1141 ctcgcggttg ccaaactcgg cgccatcatc atgccgacga ccggcgcgct cggtcccgcc
     1201 gacctgaccg accgcatcac ccgcggcggc gccaagttcg tcatcgccaa cgacgaagac
     1261 gccgccaagt tcgacgacgt gcatggcgag tacacccgca ttgtggtcgg cggcgccctt
     1321 gaagaaggag ccggatggca ccgctattcc accgcgtatg ccgtcccgtc ggccgggccg
     1381 ttcgcgacac ccaccaacac cgacgatccg atgctgatct acttcacctc gggcaccacg
     1441 agcaaaccca aactcgtcga gcactcacag gtcagctatc cagtggggca tatgtcgacg
     1501 atggcctgga tcggcgtgcg ccccggagac gtgcatctgg cgatcagctc gccgggctgg
     1561 gccaagcatg cctggagttg cttcttcgcg ccgtggatcg ccgaggcgac gatctttgtc
     1621 tacaactaca gtcggttcga cgccgccgag ctgctgaatc agctacgccg cgcacgtgtc
     1681 aacacgttct gcgcgccacc cacggtgtgg cgcatgctga ttcaatccga cctcggctcg
     1741 aaacccgaag ggctgcgcga gattctcggc gccggcgagc cgctcaatcc cgacgtcatc
     1801 gcccaagttg aacgggcgtg ggggctgacg atccgcgacg gattcggcca gaccgagacc
     1861 acgctgcagg tcggcaacac tcccggccag ccggtgaagc cgggctcgat gggccggccg
     1921 atgccggggg tgccggtcgt gctggtcgac ccggttaccg gtgagccggc cgacgagggt
     1981 gagatttgcc ttgatctgca acgcaacccg gtcaatctga tgacgggcta cctcggtgac
     2041 ccccagcgca acgccacggt gatggcgcgc ggctactacc acaccggtga cgtcgcgagc
     2101 cgtgacgatg agggctacat cacatacatc ggtcgaaccg atgacgtctt caaatcctcg
     2161 gactacaagg tgtcaccctt cgaactggag agcgtgttga tagagcatcc cgccgtggtg
     2221 gaggcggccg tcgtgccgca gcccgacgaa acccggctct gtgtgccgaa ggcatatgtc
     2281 tcgttggccg agggctggga gccgaatgcc gagaccgcaa ggcagatcat ggaatacgcg
     2341 cgcgatcacc tggcgccgta tttgaaggtg cgccgcgtcg agttctatga actgcccaag
     2401 acgatctcgg gcaagatccg ccgggtcgag cttcgccgtc gcgaggaggc tgcgcacgcc
     2461 gagggcaagt cgattgagac agagcatcgt tacgaggacg tggtgggccg gtgaagtcct
     2521 acgacgcggg tccgaccgac accccaatcc ttgagcagac catcggggag aacttcgagg
     2581 agacggttcg ccgcaatccg ggcgcggagg cgctcgtcga cgtcgcctcc ggtcgacggt
     2641 ggacgtacgg cgagctcaac gccgagatcg acctcatcgc gcggggactc atgtctcttg
     2701 gcatcgaacc cggtgagcgg gtgggcatct gggcgccgaa ctgtaccgag tggaccatgg
     2761 ttcagtacgc caccgccaag gtcggcgcga ttctggtcaa catcaacccc gcctaccgta
     2821 cgcacgaact ggcctacgtg ctgaaccagt caggtgtgcg cacgctgatc tctgcgacgg
     2881 cattcaagac atcggactat gtgggcatgg tcgaggaggt gcgccccgac actcacgtcc
     2941 tgagggacgt cgtcttcctg ggcaccgatg actggacgga gctgagaagc cgcgccgact
     3001 gcgtgaaacc gggcgagctg cgcactcgca tggcgtccct ggacaacaac gagcccatca
     3061 acatccaata cacatcgggc acaacgggtt tccccaaggg cgcaacgctg tcgcaccgca
     3121 acatcctcaa caacgggttc ttcacaaccg agctgatcaa cctccgtccc ggtgatcgcc
     3181 tgtgcatccc ggtgcccttc taccactgct tcggcatggt tatgggcaac ctgggatgca
     3241 ccagccacgg cgcgacgatg gtgattcccg cgccgggctt cgatcccggg ttgacactcg
     3301 ccgcgatcga gaaggagcga tgcaccggtg tctacggcgt gccgacgatg ttcatcgcga
     3361 tgctcggcca ccccgatttc gtgaaccgcg acttgtcgtc gctgcgaacg ggcatcatgg
     3421 cgggttccgt ctgccccgtc gaagtgatga agcggtgcgt caatgagatg aacatgaccg
     3481 aggtggcgat cgcgtacggc atgacggaga cgtcgccggt ctcctgtcag acgcacgtcg
     3541 atgacgacct cgagcggcgc acggcgaccg tcggacgcgc gcaccctcac gtggacatca
     3601 agatcgtcga cccggagacc ggtgacaccg tcgagcgggg gcagccgggc gagttctgca
     3661 cccgcggcta ctccctgatg ctgggttatt ggaacgacaa cgacaagacg cgcgaggcga
     3721 tcgacgccga cggctggatg cacaccggcg acctcgccgt catgcgagaa gacggctact
     3781 gcaacatcgt tggccgcatc aaggacatgg tgatccgcgg cgg
//
LOCUS       DASRJA010000485         6334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1236561,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000485 DASRJA010000000
VERSION     DASRJA010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236561"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VFC01_21240"
     CDS             complement(<1..681)
                     /locus_tag="VFC01_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN82186.1"
                     /translation="MSSTDVSAKLFADACAVIPGGVNSPVRAFSSVGGTPRFITSATG
                     YWLTDADDNRYVDLVCSWGPMILGHANPAVVEAVQRVAADGLSFGAPTPSETELATEI
                     INRVAPVERLRMVNSGTEATMSAIRLARGFTGRAKIIKFSGCYHGHSDALLADAGSGV
                     ATLGLPSSPGVTGAATADTIVLPYNNIPAVEEAFAQFGDEIACIITEASPGNMGTVPP
                     QPGYNASLH"
     gene            complement(820..1206)
                     /locus_tag="VFC01_21245"
     CDS             complement(820..1206)
                     /locus_tag="VFC01_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HZN82187.1"
                     /translation="MAYLKPPWFTRAVFNKIAMATGMSNTETLTVTRRRSKQRQQVPV
                     IPVDVGGTKYVVSTRGESDWVKNLRADPNATIGSTAYVAREIPEQDRQPILTAYREKA
                     GRAVEGYFRQLPREADHPVFVVTPKG"
     gene            1230..1613
                     /locus_tag="VFC01_21250"
     CDS             1230..1613
                     /locus_tag="VFC01_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN82188.1"
                     /translation="MQLPQWLARFNRHFTNPIQRMWAGWAPGMGILEHAGRKSGKQYR
                     TPLNVFPTEDGVVIVLTYGPNRDWLKNIAATGGGHMKRYGKTIELVDPEVVPKAEAAQ
                     SVKGLWRPIVARLPFESAVVLKRAA"
     gene            complement(1610..3823)
                     /locus_tag="VFC01_21255"
     CDS             complement(1610..3823)
                     /locus_tag="VFC01_21255"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZN82189.1"
                     /translation="MNTRHNQTTQNILTTGASSGGAKAIGRLGVLAVALGIGTAVTST
                     PWVAVAEPSTSDASNSSSSASSDSSDESGPPSTSDEQSSSVESHDSSLSPSGPRVVVR
                     SSGGADVSGGTDSETDDTEDVETTSTGEESLSEHDTSTASDEAEVTEPGENVVPENVV
                     SEPDESVPADSEPVVESGGPPTEPKPTSNPAPESASDPVSNAGPAARLQPASISVASA
                     LSRSTASGELVNTAETTAPDSDVLRSAAPTAPNVVAAQDVAAVMAGPPAPVSPVQVLS
                     NLVSGLFAWLGLGPSATGAPAAPVESPGLWGLLAWVRREIQRTFFNRTATINYNPDRN
                     GLTLEGDGSVSEVTGDLNAVDPDGDPVSFTVTQAPKYGTVVINRDGTFTYTPDPKFAR
                     LGLPDQFTVMAQDHGFHWHGPGGVFKPGLGHNDTAVIDIPVGPQLTPVVIATIGVGDF
                     PLDVAFTPDGGHAYVTNADSDTVSVIDTATNTVSATITVGDRPYRVAVHPDGTLAYVT
                     NYDDWTVSVIDTATNTVTDTISVGSGAGKGADYVVFSPDGTKAYVGSSNMLQVINTAT
                     NTVIDTRILGFGPYGIAVSPDGDWVYVASWTGDYVKKIRTLPNGTSFNIDVGNSPVGV
                     TMSPDGTRLYVTNADSHSVSVIDTATDTVIETIIAGNTPVEVAISPVGELAYVASQFS
                     GTIKVIDTSTNTVIKTFNLGVVTSSIAVSPDGKRAYVTDREGGTVKVMNLEPLLD"
     gene            4050..4349
                     /locus_tag="VFC01_21260"
     CDS             4050..4349
                     /locus_tag="VFC01_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82190.1"
                     /translation="MATINKAKIGSVENLAPGSTTNYQWNNPPHATVLGYFAYADPPP
                     GQGEHGSSQGSVEIVRVVHTLIKKNDGPNVERVNIHVKNTGSSATGFDLYQSWIT"
     gene            complement(4415..4576)
                     /locus_tag="VFC01_21265"
     CDS             complement(4415..4576)
                     /locus_tag="VFC01_21265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82191.1"
                     /translation="MEWAGNKILVSLSRQGPPVNPGAVDEQFTEPFTINVNGALVYNV
                     RGTYKITYE"
     gene            complement(5333..5827)
                     /locus_tag="VFC01_21270"
     CDS             complement(5333..5827)
                     /locus_tag="VFC01_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LpqH"
                     /protein_id="HZN82192.1"
                     /translation="MKRGFLVAVGGIAIVIAGLSGCSSDDKKAESPATTAEATTEAGT
                     AGATAAPSGGGTKVSIDGQDQNVSGTVVCTAMGGNMNFAIGGAATGIAAVVSEDGSNV
                     QSVALGNVNGVTLGYQSGTGQGEAKAEKSDKTWKISGTATGIDMANPMQPVNKPFEIE
                     VTCP"
     gene            6023..6220
                     /locus_tag="VFC01_21275"
     CDS             6023..6220
                     /locus_tag="VFC01_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82193.1"
                     /translation="MATNPRRDESLYRLPLPYATALRLRDAGIADDVIAERVGVDLDA
                     LPTFMRVAEAKLAAAFEDPPT"
BASE COUNT         1059 a         1966 c         2103 g         1206 t
ORIGIN      
        1 gtgcagcgac gcgttgtacc cgggctgtgg cggaaccgtt cccatgttgc ctgggctggc
       61 ctcggtgatg atgcaggcga tctcgtcacc gaactgtgcg aacgcctctt ccaccgcggg
      121 aatgttgttg tagggcaaca cgattgtgtc cgccgtggcg gctccggtga cgccgggtga
      181 ggacggtagg cccagcgtcg caacccccga tccggcgtcg gccagcaacg cgtcgctgtg
      241 gccgtggtag cagccggaga acttgatgat cttggctcgg ccggtgaaac cgcgggcgag
      301 ccggatcgcg ctcatggtgg cctcggtgcc ggagttcacc atgcgcagcc gctccacggg
      361 ggcgacgcgg ttgatgatct cggtggcgag ctcggtttcg gacggtgttg gcgcgccgaa
      421 tgacaggcca tcggcggcga cgcgctgtac cgcctcgacg acggcggggt tggcgtggcc
      481 caggatcatc ggcccccagg agcacaccag gtcgacgtag cggttgtcgt cggcgtcggt
      541 cagccagtat ccggtcgcgg aggtgatgaa ccgcggggtg ccgccgaccg aggagaaggc
      601 cctcaccggc gagttcaccc cgcctgggat gacggcgcac gcgtcggcga agagcttcgc
      661 ggagacgtcg gtgctggaca tggccaccag tgtcccagcc agcccaaagc cgtcaactac
      721 ggggtgtagt tagacacctc cgcccctgca gcgccgaccg cacggttgtt gtacacaaac
      781 gtcgagaagg tgcgcgaata agcgtgcgct cggcgttcgt cagcccttgg gggtgacgac
      841 gaacaccggg tgatcggcct cgcgcggcag ttgacgaaag tagccttcga cggcccggcc
      901 ggccttctcc cggtacgcgg tcaggatcgg ttgccggtcc tgctccggga tttcccgcgc
      961 gacgtatgcc gtcgacccga tcgtcgcgtt ggggtcggcc cgcaggttct tcacccagtc
     1021 ggattccccg cgggtcgaca ccacatattt ggtgccgccg acgtcaacgg ggatcaccgg
     1081 gacctgttgc cgttgcttac tgcgccgccg cgtcacggtc agcgtctcgg tgttgctcat
     1141 cccggtcgcc atcgcgatct tgttgaagac cgcgcgggta aaccacggag gcttgagata
     1201 ggccattgcc tacgagtttg atggtttaca tgcaactacc gcaatggctg gcgcggttca
     1261 accggcactt caccaacccg atccagcgga tgtgggcggg ctgggcacca ggcatgggaa
     1321 tcctcgagca tgccggccgg aaatcgggaa agcagtaccg caccccgttg aatgtgtttc
     1381 ccaccgagga tggggtggtc atcgtgttga cctacgggcc aaaccgcgac tggttgaaga
     1441 acattgccgc caccggcggc ggccacatga agcgctacgg caagacgatc gaactcgtcg
     1501 acccggaagt ggtgccgaag gcggaagcgg cgcaatccgt gaaaggcctg tggcgcccga
     1561 tcgttgcgag gctgccgttc gagtcggcgg tggtgctgaa gcgcgccgcc tagtcgagca
     1621 acggttccag gttcatcacc ttcacggtgc cgccttcgcg gtcggtgacg taggcgcgct
     1681 tgccgtctgg gctcaccgct atcgaggacg tgacgacgcc gagattgaac gtcttgatga
     1741 cggtgttggt ggaggtgtcg atcaccttga tggtgccact gaactggctg gcgacgtagg
     1801 cgagctcgcc caccgggctg atcgccacct ccacgggagt attgccggcg atgatggtct
     1861 cgatgacggt gtcggtggcg gtatcgatca ccgacaccga gtggctgtcg gcgttggtga
     1921 cgtagaggcg ggtgccgtcg gggctcatcg tcaccccgac ggggctgttg ccgacatcga
     1981 tgttgaagct ggtgccgttg gggagggtgc ggatcttctt cacgtagtcg ccggtccagc
     2041 tggcgacgta gacccagtcg ccgtcggggc ttaccgcgat tccgtacggg ccgaagccga
     2101 ggatgcgggt gtcgatgacg gtgttggtgg cggtgttgat cacctgcagc atgttgctgc
     2161 tgccgacgta cgctttcgtg ccatcggggc tgaacaccac ataatccgca cccttgccgg
     2221 caccggagcc gacagagatc gtgtcggtga cagtgttggt ggcggtatcg atcaccgaga
     2281 ctgtccagtc gtcgtaattg gtgacgtagg ccagtgttcc gtcggggtgg acagccacac
     2341 ggtatggacg gtcgccgaca gtgatggtgg cgctgacggt gttggtggcg gtatcgatca
     2401 ccgagactgt gtcggaatcg gcgttggtga cgtaggcgtg gccaccatcg ggggtgaacg
     2461 ccacatctag cggaaagtcg ccgacaccga tcgtcgcaat gacgacgggc gtgagctgcg
     2521 ggccgacggg gatatcgatg acggccgtgt cgttgtggcc caagccgggt ttgaacacgc
     2581 cccctggtcc gtgccagtgg aacccgtggt cttgggccat gacggtgaac tggtcaggga
     2641 ggccgaggcg ggcgaacttt ggatccggtg tgtaggtgaa ggtgccatcc cggttgatca
     2701 ccaccgtccc gtactttggg gcctgggtga cggtgaagct cactgggtcg ccatcggggt
     2761 cgacggcatt caggtcaccg gtcacttcgc tcaccgaccc gtcgccctcg agagtcaggc
     2821 cgttcctgtc ggggttgtag ttgatggtcg cggttcgatt gaagaacgtg cgctggattt
     2881 cgcggcgcac ccacgccagc agcccccaca accccggcga ctccacgggc gcggcggggg
     2941 cgccagtcgc cgatgggccc aaccccagcc aggcgaacaa ccctgacacc agattggaaa
     3001 gcacctgtac cggcgagact ggggcgggcg ggcccgccat gacggccgca acatcctgcg
     3061 ccgcaaccac attcggcgcc gtcggggccg cgctgcgcag cacatcactg tcgggcgcag
     3121 tggtttcggc ggtgttcacc agttcccccg aggctgtcga ccgcgacaag gccgacgcta
     3181 ccgatatcga cgccggctgc agccgagccg cagggcctgc gttcgacact ggatcggacg
     3241 ctgattcggg cgccggattc gaagtcggtt tgggttcggt tggcggccca ccggattcga
     3301 ccaccggctc gctgtcggcg ggtaccgatt cgtcgggctc ggacaccaca ttctcgggca
     3361 ccacgttctc gcccggttcc gtgacctccg cctcatcgga cgcagtcgat gtgtcgtgtt
     3421 cggacaacga ctcctcgccc gtcgaggtcg tctcaacgtc ctccgtatcg tcggtctcgc
     3481 tatcggtgcc gccggatacg tcagcgccac ccgaactacg cacaacgacc cgcggcccac
     3541 tgggggacaa cgacgaatca tgcgactcta ccgacgacga ttgttcgtcc gacgtcgatg
     3601 gcggacccga ttcatctgag gaatccgacg acgcactgga cgaggaattc gaagcgtccg
     3661 atgtcgacgg ttcggcaacc gcgacccacg gcgtgctcgt cacggctgtc ccgattccca
     3721 atgccaccgc cagcaccccc agccggccaa tggctttcgc gccgcccgaa ctcgcgccgg
     3781 ttgtaaggat gttctgggtc gtctggttgt gtctggtgtt catggtgggt tcccttggct
     3841 cgtgcagtgc ctcgatggcg tcatggcaag tacatagggg ccggttcgct accgatccca
     3901 aaaacacagt cgataggtct acggccgcaa cgtaaaccgc gtgtcgccgc ccaactgggg
     3961 tagtcggcta ccccagttgc tccggctgga gtaggtcacc atgggggtat cggaatcgga
     4021 tagcccaatc ccgacagcga ggagcagcga tggcgaccat taacaaggcg aagatcggca
     4081 gcgtggagaa tctggccccc gggagcacca ccaactatca atggaacaat cccccgcatg
     4141 cgacggtact cgggtacttc gcgtatgccg atccgccgcc cgggcagggc gagcacggct
     4201 cgtcgcaggg ctcggtcgaa atcgtccgcg tcgtccacac gctgatcaag aagaacgatg
     4261 gcccgaacgt ggaacgcgtg aacatccacg tcaagaacac cgggagcagc gccaccgggt
     4321 tcgacctcta tcaaagctgg atcacctagc cgcagagcgg tttagcgcga catcgatttc
     4381 agcgccgcat gcgggtcgcc cgcctgacgc tgggtcattc gtatgtgatc ttgtaggttc
     4441 cgcggacgtt gtacacgaga gccccgttga cgttgatcgt gaacggctcc gtgaactgct
     4501 cgtcaacggc gccaggattc acgggcggtc cctggcggga aagtgacacc agaatcttgt
     4561 tcccggccca ctccatgcac cctccgctgc cactggcctc ccagtgctcg tcggtagtgg
     4621 gcgggccgct gccgacggcg cagagttggc cgggatgact accgggttct ggctcgttgt
     4681 aacctccggc ggcgcggcgt gggcgaccac cggtgtggtc agcgcgacca ggagcacacc
     4741 gaaggccatt gcgaggctta cgaacccacg ctgggggccg cggccgcgtg cagtggtggt
     4801 tggtaccgca tccgcaccgg aacccgaaca tctttcgtga gggcctgtac ctgggcttgc
     4861 gatggtgtcg atgattttcc cccttttccc ttgccgcgca aacggattcg ttgcgtggcg
     4921 tgtttgtcag gaaactgaac ccgctagcgc cggccggctg acgtagtcga ctactcccgt
     4981 ttacaagcac cacggatagc accgatcgct cggtggggga ttatctccgt cccaaacatt
     5041 ggactcggga ggcggaggat attttgacgg aacgttgccc ttcccgtagt caacgaaata
     5101 ccaggtgtgg cagacgttca tgtcccaaat cttgtcgcgg cccggtcccg tcgaacccgt
     5161 ctgcggagag aacaccatgt cttgccccgg gcaccattga tatgggcccg cgttgtcggc
     5221 ctgggcggta cctgccgcca ccccaaaccc gctcgacacc aaggcggcag tcgccaaccc
     5281 tgcgatcagt gttcgctgca tgatggtttt cttcgtgagg gcggggtccc ggctatgggc
     5341 aggtgacctc gatttcgaac ggcttgttca ccggctgcat ggggttggcc atgtcgatac
     5401 cagttgcggt gccgctgatc ttccaggtct tgtcgctctt ttcggccttg gcttcaccct
     5461 ggccggtgcc ggactggtag ccgagcgtca caccgttgac gttgcctagg gcgacggact
     5521 gcacgttgga gccgtcttcg ctgaccaccg cggcgatacc cgtggcggcg ccgccgatcg
     5581 cgaagttcat gtttccgccc atcgcggtgc acacgactgt gccagaaacg ttctggtcct
     5641 ggccgtcgat ggagaccttg gttccgccgc cgctgggtgc agctgtggcg ccggcggttc
     5701 ccgcttcggt ggttgcctcc gctgtagtgg ccggactctc ggccttcttg tcgtctgatg
     5761 aacagcccga caggccggcg atgacgatcg ctatgccacc gacggcgacc aggaacccac
     5821 gcttcatgat tcgtctcctt ttcttgtgcg tccgactgag cagccgcgac cgcaagttct
     5881 acgacggcaa cgtaaagcgc atgctgtcga gcggctgggg taatcgacta ccccaattcg
     5941 gccacgaggg accggagtcg gattcagatt ggggtagcgc tttaccccag ctgggcgttg
     6001 accactgcgc gacgcttggg ctgtggccac gaacccgcgc cgggacgagt ccttgtaccg
     6061 gttgccgttg ccgtacgcga ctgcgctgcg gttgcgcgac gccgggattg ccgacgatgt
     6121 gatcgccgag cgcgttggcg tcgacttgga cgcgctgccg acattcatgc gagtagccga
     6181 ggccaagctc gcggccgcgt tcgaagaccc accaacctga tcgcgtaccg cgagtcgtca
     6241 gattgtctag gcgcgccaat ttacgcgtct gcttgaaatc gaaaagacga cgcctgtacc
     6301 agctaggatt tgccggtgat tcgggggtgg ccgc
//
LOCUS       DASRJA010000486        11225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1537119,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000486 DASRJA010000000
VERSION     DASRJA010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11225
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537119"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..426)
                     /locus_tag="VFC01_21280"
     CDS             complement(13..426)
                     /locus_tag="VFC01_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HZN82194.1"
                     /translation="MRLILNIIWLIFGGLWLALGYLLAALICFVLIITIPFGFAALRI
                     ASYALWPFGRTIVEKPGPRPGALIGNVIWVVLFGIWLAIGHVITAVAMAITIIGIPLA
                     LANLKLIPVSLVPLGKEIVPIDQARSSAVEPRAAA"
     gene            571..981
                     /locus_tag="VFC01_21285"
     CDS             571..981
                     /locus_tag="VFC01_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZN82195.1"
                     /translation="MPTGRVKWYDAEKGFGFLSQEDGEDVYVRSSALPAGVEGLKAGQ
                     RVEFGVASGRRGPQALSLKLIDPPPSLTRTRREAAAAEHKHTPDELHGMVEDMITLLE
                     GAVQPELRKGRYPDRKVARRVSEVVRAVARELDA"
     gene            1096..1302
                     /locus_tag="VFC01_21290"
     CDS             1096..1302
                     /locus_tag="VFC01_21290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82196.1"
                     /translation="MKKFVMRVIAVAAFGAAAAGLTAGTANARPLEEIKCSKIDQVIA
                     SAEQVVTAILHGKDKKEEQNRCVN"
     gene            1389..2393
                     /locus_tag="VFC01_21295"
     CDS             1389..2393
                     /locus_tag="VFC01_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HZN82197.1"
                     /translation="MAYVASGGEFNRDTNYITTRVTADGRDGYPVEPDRYRLIVARAC
                     PWANRTIIVRRLLGLEDVLSIGFCGPTHDEDSWTFDLDPGDVDPVLKIPRLKDAYLKR
                     FPDYEKGITVPAIVEVATGEVVTNDFAQITLDFSTEWAKFHRDGAPQLYPEQLRDEID
                     EVAQRIYTEVNNGVYRCGFAGSQESYDKAYDRLFTALDWLSERLQNQRYLVGDTITEA
                     DVRLFTTLARFDPVYHGHFKCNRQKLSEMPVLWAYARDLFQTPGFGDTIDFVQIKQHY
                     YIVHKDINPTQIVPKGPSLSNWLTPHGREALGGRPFGVGAPPGPTRDGERVPDGHGAG
                     "
     gene            complement(2390..2872)
                     /locus_tag="VFC01_21300"
     CDS             complement(2390..2872)
                     /locus_tag="VFC01_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2771 domain-containing protein"
                     /protein_id="HZN82198.1"
                     /translation="MNRVLAALAIVVVVASVLTGVLIWRLAQHPPPEHPEISAFSDGQ
                     LVRVGPYRYCEVLNPTDCETPGTDGELTVGPRHPVQLSVPTAIARAPWVLVRSYEGGD
                     AVDEFRPDTKLAVTVPTVDPHRGRLFGLAVQLPTLVRDEAGNEFPVPHAEWSVRTVWP
                     "
     gene            complement(2869..4458)
                     /locus_tag="VFC01_21305"
     CDS             complement(2869..4458)
                     /locus_tag="VFC01_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN82199.1"
                     /translation="MANYPSEPGVDGGYRRPRRSAPSANRWLPPLEERRARQHPSDPP
                     PPTVGASPGQKVTVTRAAAQRSREMGSKMYGLVHRAATADGADKSGLTALTWPVVANF
                     AVDAAMAVALANTLFFAAASGESKGKVALYLLITIAPFAVIAPLIGPALDRLQHGRRV
                     ALAMSFILRTGLVVLLIANYDGATGSYPSWVLYPCALGMMVLSKSFSVLRSAVTPRVL
                     PPTIDLVRVNSRLTTFGLLGGTMVGGAIAAGAEWAFGLVHLPGALYVIVAVSLVGAAL
                     AMHIPKWVEVTAGEVPATLSYHGRSGELRRHPEQHKPPATARQPLGRNIIAALWGNCT
                     VKVMVGFLFLYPAFVAKSHDASGWEQLRILGVIGAAAAIGNFAGNFTAARLKLGYPAL
                     LVVRCTIAVTTAALATALTGKLMVAALATLVTSAAAAIAKASLDASLQDDLPEKSRAS
                     AFGRSESLLQLAWVAGGAMGVLVYTDLWVGFTAITAILIVGLAQTVVSYFGESLIPGF
                     GGNRPVLAEQEGGRPAAVAPE"
     gene            4612..5370
                     /locus_tag="VFC01_21310"
     CDS             4612..5370
                     /locus_tag="VFC01_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HZN82200.1"
                     /translation="MDTVDTEDRPDLEAVLMGAVEQARAAIIEFSGEEALGEYLGASF
                     EDPTAATHRFLADMPGYRGWQWAVVVAACPGATHATISEVVLVPGPTALLAPKWVPWQ
                     ERVKPGDLSPGDLLAPPANDPRLVPGYSASGDPQVDEVAVEVGLGRRQVLGELGRSDA
                     AQRWHDGDFGPGSAMARSTRRVCRDCGFYVPLAGALGTMFGVCANELAADGHVVDSEY
                     GCGAHSDTPQPPGTGSPLYDPFDDGVLDVVEPEN"
     gene            complement(5367..5819)
                     /locus_tag="VFC01_21315"
     CDS             complement(5367..5819)
                     /locus_tag="VFC01_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN82201.1"
                     /translation="MAAPLLQAQIDINAPVSKVWALVSDLSRMPQWSPQCRLMKAFGP
                     VRQGARTINVNRRNRMFWPTTSVVTEVIPEKKLAFRVNQNNTIWSYELEPTDIGTRLV
                     ETRHAENGTTAFSNMSINALLGGVPSFERELVDGMNETLSRIKAAAES"
     gene            complement(5838..6077)
                     /locus_tag="VFC01_21320"
     CDS             complement(5838..6077)
                     /locus_tag="VFC01_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2530 domain-containing protein"
                     /protein_id="HZN82202.1"
                     /translation="MAPEPPALPAILLKPWPVIVVITAGWLIALVLAFTVPDLHAWRP
                     FTVAGLAIGALGTSIMLWQRRAVRRGSRGAQSGLS"
     gene            6137..6568
                     /locus_tag="VFC01_21325"
     CDS             6137..6568
                     /locus_tag="VFC01_21325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN82203.1"
                     /translation="MKDVDARLASELSLAVIRLARQLRFRRPDSPVSLSQLSALSTLA
                     KEGPMTPGALATRERVRPPSMTRVIASLCELGFVARSAHPADGRQVLVSVSRAGTDLI
                     AAERRASQEWLRQRLDRLEPDQRKTLLVAADLMSDIVDESA"
     gene            6565..7383
                     /locus_tag="VFC01_21330"
     CDS             6565..7383
                     /locus_tag="VFC01_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZN82204.1"
                     /translation="MTANVIDVADPADPRLDDFRDLNSVDRRPDLPSGKGLVIAEGVL
                     VVQRMLVSRFRPRALLGTDRRLSELAPDLADVDAPYYRVQAGVMAEVVGFHLNRGVLA
                     SASRPPELTVAQVLDGARTVAVLEGVNDHENLGSIFRNAAGLGVDAVIFGMGCADPLY
                     RRSVRVSMGHALLVPYAWTETWPRDLQVLRDNGFRLLAMTPDPAAYTLAEAMSETAEQ
                     KVAILVGAEGPGLTEWVMRASDMRVRVPMSRGTDSLNVATAAALAFYDRIRSAR"
     gene            7397..8254
                     /locus_tag="VFC01_21335"
     CDS             7397..8254
                     /locus_tag="VFC01_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN82205.1"
                     /translation="MKIRIREATPDDANECGRICHDAFAAIAEYHRFPPDFPTVDIAI
                     GAVSGLIAHPGFFGVVAEDDGQILGSNFLDERSMIRGVGPITVDPDVQNRRIGLALMD
                     AVLQRGAQHRAPGVRLLQSAYHNRSMCLYAKLGFDVREPFAVMQGNPLGLKVPGAAVR
                     PATDADAQACDAVCVRVHGHDRDGEVRDAIAQGTAMVVERNDRITGYTTAVALFGHSI
                     AETNDDLMALIGAAEEFGGPGFLVPMRNTELLRWCLAHGLRVVSMMNLMTIGLYQEPR
                     GAFLASVLY"
     gene            8305..8559
                     /locus_tag="VFC01_21340"
     CDS             8305..8559
                     /locus_tag="VFC01_21340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2537 domain-containing protein"
                     /protein_id="HZN82206.1"
                     /translation="MGLTVAAFVAAVTAAAVVVLSLGLTRVHPLLAVGLNLVAVGGLA
                     PTVWGWRKLPVWRWFVLGSGVGVAGAWLALMALALGSLTR"
     gene            complement(8549..9355)
                     /locus_tag="VFC01_21345"
     CDS             complement(8549..9355)
                     /locus_tag="VFC01_21345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZN82207.1"
                     /translation="MISLGPIGLASSYPYTDDAAVVADEAQRVESLGFSTLWRSGNLP
                     MLDAAVRATTHIPVATGIIPVDSVPSADVIATYRLLQRDHPGRFIVGLGGAHGAQPLK
                     TLNTYLDALDDAGVPADSRVLAALGPNMLALARDRAGGAYPFLVTPSYVTDARAALGA
                     DKTLAVLLMVMPRADREAARRAAATPLDFLTKVGGYRRNLLRQGFSESDIDDVSDRLL
                     DGITASGELDSIAARVAEYRAAGADQVVIRLLGADDEPVWQRRLAEALIE"
     gene            complement(9398..10174)
                     /gene="sepH"
                     /locus_tag="VFC01_21350"
     CDS             complement(9398..10174)
                     /gene="sepH"
                     /locus_tag="VFC01_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HZN82208.1"
                     /translation="MRELKVVGLDVDGKRIICETDDSGDKFLLRPDDRLRAALRGEKA
                     GSSQSQSDPEVPSVLRPKEIQAKIRAGASVEQIASAAGVDIVRVQRFANPVLLERSRA
                     AELATAAHPVLADGPSVLTLLETVTSALVARGLDPDATSWDAWRNEDGRWTVQMGWKA
                     GMSDNVAHFRYTPGAHGGTVTAFDDSACELIDPDFARPLRPLAPVAELDFEESEPELP
                     IEAPEPPPAPAPKPPRVRKGKPPVPAWEDVLLGVRSGGQR"
     gene            complement(10227..10820)
                     /locus_tag="VFC01_21355"
     CDS             complement(10227..10820)
                     /locus_tag="VFC01_21355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN82209.1"
                     /translation="MPANCARTSDSDYAQRFVEQAMPFVDQLYSGARRLTSSQYDAED
                     LVQETMLSGFKGFRLYREGTNLRAWLFRVMYNTWVSGHRARQSRPNEVLCDEFTDGQL
                     AHAGHIPAGLRSAEIEALERMPDADIVAALDQLPVVVRMTVYFADVEGYRYREIADLT
                     NVPVGTVMSRLARGRRRLRELLANASDLGRVYRVTGR"
     gene            10941..11177
                     /locus_tag="VFC01_21360"
     CDS             10941..11177
                     /locus_tag="VFC01_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82210.1"
                     /translation="MNARKVRAFSAIVASCAVGVGAVVVAATEPIHSAAGSTMQTGVT
                     ETATTPPTAPAIGKAAPIVTGPAPLPSEEQGLPG"
BASE COUNT         1895 a         3672 c         3803 g         1855 t
ORIGIN      
        1 gcgcgatgac ggtcatgcgg cggcccgggg ttcaacggct gacgaacgcg cttgatctat
       61 gggaacgatc tccttgccga gcggcaccag cgaaaccggg atcagcttga ggttggccag
      121 cgccagcggg atgccgatga tcgtgatggc catcgccacc gcggtgatca catggccgat
      181 ggccagccag atgccgaaca ggacgaccca gatgacgttg ccgatcagcg cgccgggccg
      241 tggtcccggc ttctcgacga tggtgcgccc gaatggccac agcgcatagg acgctatgcg
      301 cagcgcggcg aacccgaacg gaatcgtaat gatgaggacg aagcagatga gcgcggccag
      361 caggtagccc aacgccagcc agaggccgcc gaagatcaac cagatgatgt tcagaatcag
      421 gcgcatcgct cctccaacag tggtttcaga ctaccgagta aagggggtgc tcggcctcgc
      481 ggtgtccgag taggatcacc taaacgcgtc ccggcgcagg cgggacgctt tccttatgta
      541 gtcgttgtta gagacaagcg ggtgagacca gtgccgaccg gccgggttaa gtggtacgac
      601 gccgaaaagg gcttcggctt tttgtcgcaa gaggacggtg aggacgtcta cgtccggtcc
      661 tcggcgttgc ccgctggtgt cgagggcctc aaggctgggc agcgcgtcga gttcggggtg
      721 gcctctgggc ggcgtggtcc acaggcgttg agcctcaagc tgatcgaccc gccgccgagc
      781 ctgacgcgga cccgtcgcga agcggcggcc gccgagcaca agcacacgcc cgacgaactg
      841 cacggcatgg tcgaagacat gatcacgctg ctggaagggg ctgtgcagcc ggagctgcgc
      901 aagggtcgct atccggaccg caaggtcgcc cgtcgcgttt ccgaagtggt gcgcgctgtc
      961 gcccgcgaac tcgacgccta gggcgccgtc gcacgcaaaa taggtaactg ctcccgatgg
     1021 gcgatttcgc ctgggcggag agggtgtttc cggcggctgg acacaaggtt cggccgatca
     1081 gacaaggaga cactcatgaa gaagttcgtc atgcgcgtga ttgcggtggc tgcattcgga
     1141 gctgcggccg caggtctgac cgccggcacc gccaacgctc gtccgttgga agagatcaag
     1201 tgctcgaaga tcgaccaggt gatcgcttcg gccgagcagg tggtcacggc gatcctgcac
     1261 ggcaaggaca agaaggaaga acaaaaccgc tgcgtcaact gagagcaccc cgcgacgaac
     1321 gctccttact gctcgaaaaa tcgaaatatc gagcggtaag gaacgttcgg gtggggcatt
     1381 ctggtggcat ggcatatgtc gccagcggtg gcgagttcaa ccgcgacacc aactacatca
     1441 ccacccgcgt cacggccgac ggccgcgacg gctatcccgt cgaaccggac cgctaccggc
     1501 tgatcgtcgc tcgggcgtgc ccgtgggcca atcgcacgat catcgtgcgg cggctgctcg
     1561 gcctggaaga cgtgctctcc attggctttt gcgggcccac gcacgacgag gacagctgga
     1621 cgttcgacct cgatccgggg gacgtcgacc cggtactgaa aatcccccgg ctcaaggacg
     1681 cctacctcaa gcgcttcccg gactacgaga agggcatcac ggtgcccgcg atcgtcgagg
     1741 tggcgacggg cgaggtcgtc accaacgact tcgcgcagat cacgctggac ttctccaccg
     1801 agtgggcgaa gtttcaccgc gatggtgccc cgcagctgta ccccgagcag ctgcgtgacg
     1861 agatcgacga ggtcgctcag cgtatctaca ccgaggtcaa caatggcgtc taccgctgcg
     1921 gattcgccgg atcgcaggag tcctacgaca aggcctacga ccggttgttc acggcgctcg
     1981 actggctctc cgagcgcctg cagaatcagc gatacctggt cggtgacacc atcaccgagg
     2041 ccgacgtccg gttgttcacg acgctggctc gcttcgatcc cgtctatcac gggcacttca
     2101 aatgcaaccg gcagaaactc tccgagatgc cggtgctgtg ggcctacgcg cgggatctgt
     2161 tccagacgcc aggattcggc gacacgatcg acttcgtgca gatcaagcag cactactaca
     2221 tcgtgcacaa ggacatcaat ccgacgcaga tcgtccccaa ggggccctcg ctgtcgaact
     2281 ggttgacgcc gcatggtcgg gaagcattgg gcggccggcc atttggtgta ggagcgccgc
     2341 cggggccgac gcgcgatggt gagcgggtgc cggacgggca cggtgcgggc tagggccaga
     2401 ccgtgcgtac cgaccactcc gcgtgcggca cggggaactc gttgcctgcc tcgtcgcgga
     2461 ccagggtcgg cagctgcacc gcaaggccaa acagcctgcc ccggtgcgga tccaccgtgg
     2521 gcacggtcac cgcgagcttc gtgtccggcc gaaattcatc aactgcgtcg ccaccctcgt
     2581 aggagcgcac caagacccac ggcgcgcggg ctattgcggt cggcaccgac aactgcacag
     2641 ggtgacgcgg gccgacggtg agttcgccgt cggtgcccgg cgtttcacag tcggtcgggt
     2701 tgagcacttc gcagtaccga tacggtccca cccgcaccag ctgcccgtcc gaaaatgcgc
     2761 tgatctcagg atgttccggt ggcggatgtt gagcgaggcg ccagatcagc acgccggtta
     2821 acacagacgc gacgaccacc acgatcgcca gcgcggcgag gacgcgattc actccggcgc
     2881 caccgccgcc ggcctgccgc cctcttgctc ggccagcaca ggacggtttc cgccgaagcc
     2941 gggtatcagc gactcgccga agtagctcac tactgtctgc gcaagcccga cgatcagtat
     3001 cgcggtgatc gccgtgaagc ccacccacag atcggtgtac accagcaccc ccatggcgcc
     3061 gccggccacc caggccaact gcagcaacga ctctgaccga ccgaatgccg acgcacgcga
     3121 cttctccggc aggtcgtcct gcagcgacgc gtccagcgag gccttcgcga tcgctgccgc
     3181 ggcggaggtg accagcgtgg cgagcgcggc caccatcagc ttgccggtga gcgcggtggc
     3241 cagcgcggcg gtggtgaccg cgatcgtgca ccgcaccacc agcagcgccg gatagccgag
     3301 cttcagccgg gcggcagtga agttgcctgc gaaattgccg atcgccgccg ccgcgccgat
     3361 cacaccgagg atccgcagct gctcccaccc gctggcgtcg tgtgacttcg ccacgaacgc
     3421 ggggtacagg aacagaaatc cgaccatcac ctttaccgtg cagttgcccc acagcgcggc
     3481 aatgatgttg cggcccaacg gttgtcgcgc ggtggcaggc ggcttgtgct gttccggatg
     3541 gcggcgcaat tccccgctgc ggccgtggta ggacagtgtg gcgggcacct cgccggcggt
     3601 cacctcgacc cacttcggga tgtgcatcgc cagcgccgcg ccaacgagcg agaccgcgac
     3661 gatgacgtac agcgcgccgg gcagatggac gagaccgaac gcccactccg caccagcggc
     3721 gatcgcgccg cccaccatcg ttccgcccag caggccgaac gtcgtcagcc tggagttcac
     3781 tcgcaccaag tcgatcgtcg gcggcagcac acgcggagtg accgcgctgc gcagcacgct
     3841 aaatgacttg gacagcacca tcattcccag tgcacagggg tacaacaccc acgacgggta
     3901 gctgccggtc gcaccgtcgt agttggcgat cagcagcacc accagcccgg tgcgcagaat
     3961 gaaggacatc gccagcgcaa cccgccgacc gtgctgcagc cggtccagcg ccgggccgat
     4021 cagcggggcg atgaccgcga acggtgcgat ggtgatgagt aggtatagcg cgaccttgcc
     4081 tttgctctcc cccgacgccg cggcgaaaaa gagcgtgttg gccaatgcga cggccatcgc
     4141 cgcgtcgacg gcgaagttgg ccaccacggg ccacgtcaac gcggtcaggc cggacttgtc
     4201 cgcgccgtct gcggtggcgg cccgatgcac caatccgtac atcttcgaac ccatttcgcg
     4261 gctgcgctgc gctgccgcgc gggtgacggt gaccttctgg cccgggctcg cgccgacggt
     4321 gggcgggggc ggatcggatg ggtgctgacg ggcacggcgc tcctcgagcg gcggcagcca
     4381 ccgattcgcg ctcggcgccg accggcgcgg tcggcggtag ccgccgtcga caccgggttc
     4441 actgggatag ttcgccatcc cgggatgctc atcggacggc gggcgcggcg ggtagtagcg
     4501 agggtcacgc cctgatccgg tcacgccacg attctccccc atcacagcgg tggcgggcgt
     4561 gcaggtgacc ggtgtggctc gacgttcccc ggtcaggcac tattgatggc gatggacacc
     4621 gtggacaccg aagatcgccc cgacctggag gcggtgctga tgggcgccgt cgagcaggcc
     4681 cgcgcggcga tcatcgagtt cagcggtgag gaagccctgg gcgagtatct cggcgccagc
     4741 ttcgaggacc cgaccgccgc cacgcatcgg ttcctggccg acatgccggg ctaccggggc
     4801 tggcagtggg ccgtcgtcgt ggccgcctgc ccgggagcaa cccacgccac gattagcgaa
     4861 gtcgtgttgg tgcccggccc gacggcgttg ctggcgccta agtgggtgcc gtggcaggaa
     4921 cgcgtcaagc ccggcgacct gagccccggt gatctgttgg cgcccccagc caacgaccca
     4981 cgactggtgc cgggctattc ggccagtggt gacccgcaag tcgacgaggt cgccgttgag
     5041 gtgggcctgg gccgccgtca ggtgctcggc gagttgggcc gcagcgacgc cgcgcaacgg
     5101 tggcacgacg gagatttcgg cccaggttcg gcgatggccc ggtccacccg ccgcgtctgt
     5161 cgcgactgcg gcttctacgt cccgctggca ggcgcgctcg gcacgatgtt cggcgtatgc
     5221 gccaacgagc tggcggccga cgggcatgtc gtcgactccg aatacggttg cggcgcacac
     5281 tcggacacac cgcagccgcc cggcacgggg tcgccgctgt acgacccgtt cgacgacggt
     5341 gtgctcgacg tggtcgagcc cgagaactaa ctttccgctg cggccttgat gcgcgacagc
     5401 gtctcgttca tgccgtcgac gagttcgcgc tcgaaactcg gcaccccacc cagcagggcg
     5461 ttgatgctca tgttcgagaa cgcggtggtg ccgttctcgg catgacgggt ttccaccagc
     5521 cgcgtgccga tgtccgtcgg ctccaattcg taactccaga tcgtgttgtt ctggttcacc
     5581 cggaacgcca gcttcttctc cgggatgacc tcggtgacga cgcttgtcgt cggccagaac
     5641 atccggttgc gacggttgac gttgatcgtc ctggcgccct ggcgcaccgg gccgaacgcc
     5701 ttcatcaacc ggcattgcgg actccactgc ggcatccggc tgaggtcgga gaccaacgcc
     5761 cacaccttcg agaccggtgc gttgatgtcg atctgtgctt gtaacagcgg tgcggccatt
     5821 cccctccttg gtcgaattca gctgagcccg ctctgcgcgc cccgcgatcc gcggcgtacg
     5881 gcgcggcgtt gccacagcat gatcgacgtg ccgagcgcgc cgatggccag gccggcgacc
     5941 gtgaacggcc gccacgcgtg caggtcgggc accgtgaacg ccaacacaag cgcaatcagc
     6001 caacccgcgg tgatcaccac gatgaccggc cacggcttga gcaggatggc cggcagggcg
     6061 ggcggttccg gtgccacgct gacaacgtag ccgataacgg cgactttccc gagacgtgct
     6121 aggtactctt tgccttgtga aagacgtcga cgcccggctt gcgagtgagc tgtcgctggc
     6181 agtgatccgt ctggctcgcc aattgcggtt ccggcgtccg gattcgcctg tttcgctttc
     6241 gcagctctcg gcgttgtcga cgcttgctaa ggagggcccg atgacgcccg gcgcgttagc
     6301 cacgcgcgag cgggtacgcc caccgtcgat gacacgggtg atcgcgtcgc tgtgcgagct
     6361 gggattcgtc gcgcgctccg cgcaccccgc cgacggccgt caggtgctgg tctcggtgtc
     6421 ccgcgcgggc acggacctga tcgcggccga gcgccgcgca agccaggagt ggctcaggca
     6481 acgcctcgac cgtctcgaac ccgatcagcg caagacattg ttggtggccg ccgatctgat
     6541 gtccgacatt gttgacgaaa gcgcgtgact gccaacgtaa tcgacgtcgc tgaccctgct
     6601 gacccgaggc tcgacgactt ccgcgatctc aacagtgtgg atcgcaggcc cgatctgccg
     6661 agcggaaagg gtttggtgat cgccgaaggg gtgctggtgg tgcagcgcat gctggtctcc
     6721 cggttccggc ccagggctct gctcggcacc gaccggcggc tctccgaact tgcgccggac
     6781 ctggcggacg tggacgcgcc gtattaccga gtgcaggccg gcgtgatggc cgaggtggtc
     6841 gggttccacc tcaaccgcgg agtgctggcg tcggcgtccc gcccgcccga gctgaccgtg
     6901 gcgcaggtgc tcgacggtgc ccgcacagtc gcggtgctcg agggcgtgaa cgaccacgag
     6961 aacctcggct cgatcttccg caacgccgcc ggcctcgggg tggacgcggt catcttcggc
     7021 atgggttgtg ccgacccgct ttaccgccgc tccgttcggg tgtcgatggg tcacgccctg
     7081 ctggtcccgt acgcgtggac cgagacctgg cctcgggacc ttcaggtatt gcgggacaac
     7141 ggttttcgcc tgttggcaat gacacccgat ccggccgcgt acactttggc cgaggcgatg
     7201 agcgaaaccg cggagcaaaa ggtggcgata ctcgtcggcg cagaggggcc ggggctgacc
     7261 gagtgggtta tgcgggccag cgacatgcgg gtgcgggtcc cgatgtcgcg aggaaccgac
     7321 tcgctcaacg tcgccacagc cgctgcactg gcgttctacg atcgcatcag atcggcgcgg
     7381 tagcaggggg tgcgtcgtga aaattcggat tcgagaagca acgcccgacg acgccaacga
     7441 gtgcgggcgc atctgtcatg acgccttcgc cgccatcgcc gaatatcatc gcttcccgcc
     7501 cgacttcccg acggtcgaca tcgcgatcgg ggcggtgtcc ggcctcatcg cacatccggg
     7561 gttcttcggt gtcgtcgcgg aggacgacgg gcagatactc gggagcaact tcctcgacga
     7621 acggtcgatg attcgcggtg tcggccccat caccgtcgac cccgacgtgc agaaccgtcg
     7681 gatcgggctg gcgttgatgg acgcggtgct tcagcggggc gcccagcatc gcgcaccagg
     7741 ggtgcggttg ttgcagtcgg cgtatcacaa ccggtcgatg tgcctgtacg cgaagctcgg
     7801 gttcgacgtc cgtgaaccgt tcgccgtcat gcaaggaaat ccattggggc tcaaggtgcc
     7861 tggcgctgct gttcgaccag caacagatgc ggatgctcag gcctgcgacg ccgtgtgtgt
     7921 gcgtgtgcac ggccacgacc gggacggtga agtccgcgac gcgatcgcgc agggaacggc
     7981 gatggtggtc gagcggaacg acaggatcac gggatacacc accgccgtgg cactgtttgg
     8041 gcactcgatc gccgagacca acgacgatct gatggcgctg atcggcgcgg ccgaggagtt
     8101 cggcggtccc ggctttctcg tgcccatgcg caataccgag ctcttgcggt ggtgtctcgc
     8161 gcatgggctg cgggtggtgt cgatgatgaa cctgatgacg atcgggctct accaggagcc
     8221 acgcggcgca ttcctggcct cggtgctgta ctagcggtct ccggctaggt tggcggagtg
     8281 accgacgact cgactccgtg ggctatgggt ttgacggtgg cggccttcgt cgcggcggtg
     8341 acggccgcgg cggtcgtggt tctgagcctg ggcttgaccc gcgtacatcc gctgttggcc
     8401 gtcgggctca acctcgtggc cgtcggcggg ctcgccccaa cggtgtgggg gtggcgcaag
     8461 ctgccggtct ggcggtggtt cgtgctgggc tccggcgtcg gggtggcggg cgcgtggctc
     8521 gccctgatgg cgctggctct tggcagtctc actcgatgag cgcttcggcc agcctgcgct
     8581 gccacaccgg ctcgtcgtcg gcaccgagga gccggatcac cacctgatcg gcaccggcgg
     8641 cgcggtattc cgcgacgcgc gccgcgatcg aatcgagttc gcccgatgcc gtgatgccgt
     8701 ccagaagccg atcgctcacg tcgtcgatgt cggattcaga aaagccttgc cgcaacaggt
     8761 ttcggcggta tccgccgacc ttcgtcagga agtccagcgg tgtggcggcc gcccgtcgcg
     8821 cggcctctcg gtcggctctc ggcattacca tcagcagtac tgccagcgtc ttgtccgcgc
     8881 cgagggcggc gcgcgcgtcc gtcacgtacg acggcgtcac gagaaacgga tacgcgccgc
     8941 cggcacgatc gcgcgccaat gccaacatgt tcgggcccag cgcagccagt acccgagagt
     9001 cggcggggac gccggcgtcg tccagcgcgt cgaggtaggt gttgagcgtc ttcaacggct
     9061 gcgctccatg cgcaccgccg aggccaacga tgaagcggcc ggggtgatcg cgctgcagca
     9121 accgatacgt cgcgatgaca tccgccgacg gcaccgaatc cacggggatg atgccggtgg
     9181 ccaccggaat atgcgtcgtg gcccgcaccg ccgcgtcgag catcggtagg ttgccggatc
     9241 gccacagcgt cgaaaacccc aggctctcaa cccgttgcgc ttcgtcggcg acgacggctg
     9301 catcgtcggt gtacggatag ctactggcga gcccaatcgg gccgaggctg atcatgaggt
     9361 cgaggttagg acctcaacga cggttcaggt caaggcgtca gcgctgcccg ccggagcgca
     9421 cgccgagcag cacgtcctcc cacgccggga ccggcggctt acccttgcgc acccgcggcg
     9481 gcttcggcgc gggtgccggc ggcggctcgg gggcttcgat gggcagttcc ggctccgatt
     9541 cctcgaagtc gagttccgcg accggagcca gtggccgcag cggccgggcg aagtcggggt
     9601 cgatcagctc gcacgctgaa tcgtcgaacg cggtcacggt gccgccatgt gcgcccggcg
     9661 tgtagcggaa gtgcgcgacg ttgtcggaca tgccggcctt ccagcccatc tgcaccgtcc
     9721 agcggccgtc ctcgttgcgc cacgcgtccc agctggtggc atccggatcc agcccgcggg
     9781 cgaccagcgc cgacgtcacc gtctccagca gcgtcaggac cgagggaccg tcggcgagca
     9841 cggggtgcgc ggcggttgcc agctcggccg cgcgggagcg ctccagcaat accgggttgg
     9901 caaaacgctg gacgcggacg atgtcaacac cggcagccga ggcgatttgt tcgacggacg
     9961 ccccggcccg gatcttggct tgaatctcct tgggacgcag cacgcttggg acctcggggt
    10021 cggattggga ttggctcgag ccggcctttt cgccgcgcag agcggcgcgc aaccggtcat
    10081 cgggccgcaa caggaacttg tcgccggagt cgtcggtctc gcagatgatt cgtttgccgt
    10141 caacgtcgag tccgacgact ttgagttccc gcatatcgac ctcctccggg ccaggagcca
    10201 agcccgatta ggaggaccct actgtgttat ctgcccgtta cccggtagac acgcccgagg
    10261 tcagaggcgt tggcgagcaa ctcgcggagg cgtcgacggc cgcgcgcgag gcgcgacatc
    10321 acggtgccga ccgggacgtt cgtcaggtcc gcgatctccc gatagcggta gccctcgacg
    10381 tccgcgaaat agaccgtcat ccgaacgacg acaggtagct ggtcgagtgc ggcgacgatg
    10441 tcggcatccg gcattcgttc caacgcttcg atctcggcgg accgcagacc cgccgggata
    10501 tggccggcgt gggccagctg cccgtcggtg aactcgtcgc acagcacctc gttcggcctg
    10561 ctttgccggg cgcgatgacc gctgacccag gtgttgtaca tgacgcgaaa cagccacgcg
    10621 cgaaggttgg tgccctcgcg atagagccga aaacccttga acccgctcag catcgtttcc
    10681 tgaacaaggt cttctgcgtc gtactgagag gaggtcaacc ttcgcgcacc gctgtagagc
    10741 tggtcgacga aaggcatcgc ctgctcgacg aagcgttgcg cgtagtccga gtcgctcgtg
    10801 cgtgcgcagt tagctggcat ctcggtcttc agcggtagcg ggaagttaca tagacaaaca
    10861 aagtgtgtaa aggcgaatat tccgcgatcg ccatccgatt cacctaaggg agtgacgcct
    10921 tcgggggcga aggagtagca gtgaacgcac gtaaggtgag ggccttcagc gcaatcgtgg
    10981 cgagttgcgc cgtcggtgtt ggtgccgtcg tagtcgcggc aacagagccc attcattcgg
    11041 cggccggttc gaccatgcaa accggcgtga cagaaaccgc gacaacgccg cccaccgccc
    11101 cggcgatcgg taaggccgcg ccgattgtca ccggtccggc tccgctgcct agcgaagagc
    11161 aggggctgcc cgggtagcgc tcgctagagg cgttctacca cccaatcgat gcactgcgtg
    11221 agcgc
//
LOCUS       DASRJA010000487        35193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3749120,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000487 DASRJA010000000
VERSION     DASRJA010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 35193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35193
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3749120"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..450)
                     /locus_tag="VFC01_21365"
     CDS             complement(79..450)
                     /locus_tag="VFC01_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HZN82211.1"
                     /translation="MAVAVIGVIAVFFLGMGVYALAAPAALVRPFGITLGESASRSEV
                     RAVYGGFGLAIAGVLVHAAVADGDFRKGILIAVGAALAGMAFGRLVAAVLGDRTAFYP
                     NWFYCVVEAMAAAALFAISAR"
     gene            486..1247
                     /locus_tag="VFC01_21370"
     CDS             486..1247
                     /locus_tag="VFC01_21370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN82212.1"
                     /translation="MTAKAAPALPQTCCPSVWLWPGQALYAGPSLNLAPHSGSVWCLA
                     VGIDGALSVSTKDGIKRTAHSVLIPPRLTHQLTCHGAGLVSCYLEPTSERADTGRHKF
                     GEWHGEVAVRHVDEERLQFTPTDDESASHWLDLAAPAAPRQVDPRIAAAARRIREDPA
                     AAISSRELAAQAGLSESRFLHLFRDELGTSVRRYRLWARLTHAGAAIAAGDNLTTAAM
                     KAGFASPSHLADRFKTTFGLSASRLLGTGLTLRAP"
     gene            complement(1204..2697)
                     /locus_tag="VFC01_21375"
     CDS             complement(1204..2697)
                     /locus_tag="VFC01_21375"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN82213.1"
                     /translation="MSEPEYSDVLIIGAGISGIGAAYRIRQRNPRLSYTILERRARVG
                     GTWDLFRYPGIRSDSDIYTLCFPWEPWTRPEMIVDGEHIWEYLDQTARKYGIDKHIRF
                     HSYVRSADWDSTTDTWTVRAEQDGQLATYQSRFLFFGTGYYNYDEPYAPTFPGIEDFE
                     GEVVHPQLWPESFEYEGKRIVVIGSGATAVSLIPSLTDTAAHVTMLQRTPSYMISATK
                     IEPTALAIRKVLPLKVAHWIVRWRNAVIGAILWLVARRAPEFSKRFLRRIAEHTLPAG
                     YDIDTHFTPPYNPWDQRLCFIIGADLYKEIAKGRVEMVTDHIDHMDASGIVLRSGRRL
                     DADVIVTATGLQLQSLGGATITVDGQKVDPHDRFLYRRHMLEDVPNAAWCVGYTNASW
                     TLGADLTAQSFAKLLAYMDSHGYTRAYAHLGNVDMPEQPAFNLDSGYVKRGLHLLPKS
                     GTRRPWLMSHNFLRDVVGRPFESIEESMVFGRAATEPAGSAPSRAVG"
     gene            complement(2709..4190)
                     /locus_tag="VFC01_21380"
     CDS             complement(2709..4190)
                     /locus_tag="VFC01_21380"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN82214.1"
                     /translation="MGAHDAEYSDVLIIGAGISGIGAAYRIHEKNPGLSYTVLERRQR
                     IGGTWDLFRYPGIRSDSDIFTLSFPYEPWTRPENVADGPDIRAYLTETARKNGIDEHI
                     RFNTRVISADWDSATDTWTVETEQDGMPKSYRGRFLFFGTGYYNYDEPYRPEFPGIET
                     FTGEVVHPQFWPEGFDYTGKRVVVIGSGATAVSMIPSLTDQAGHVTMLQRTPTYMLSM
                     PRIHPVVQAIRKVLPMRAANHAVRLYNTFFTVMTYVVARKAPRFSRWLIRAMAKKSLP
                     EDYPVDIHFRPPYDPWDQRLCLILDNDLYAAVGDGRADVVTDTIDHIDSSGIVLKSGP
                     HVDADVIITATGLQLQALGGVSLSIDGAEVKPQDRFVYKEHMLEDIPNLAWCVGYINA
                     SWTLRADLTARAVAKLLAYMGSHGYTHAYPHLGDKPMPEKPAWNINAGYVQRAAHALP
                     KSGTHRPWNVRHNYVLDAIDHRFDRIEESMVFGRAEAREVEPA"
     gene            complement(4288..4992)
                     /locus_tag="VFC01_21385"
     CDS             complement(4288..4992)
                     /locus_tag="VFC01_21385"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN82215.1"
                     /translation="MGGYRPGFVVAADADGREVARINDAPKALAAGLKRQQRLGKSLS
                     RKQKGSRNRKDAAAKLGRHHHHVANVRRHFLHQVSNELVKTHDRLVIENLNVTGMLGN
                     HHLARAISDAGWAEFARILAYKQAWRGGELAVADRWYPSSKLCPQCGAVRDDLTLADR
                     VFNCGCGHSADRDRNAATNLACWGQTHYLDPRTPKHGGRATNARRRDGADQHPSCAGE
                     TSPVDAGTDVHTAPAA"
     gene            complement(4935..5504)
                     /locus_tag="VFC01_21390"
     CDS             complement(4935..5504)
                     /locus_tag="VFC01_21390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82216.1"
                     /translation="MEKTEDGGRVVAVDADGVAEVVVTGLEWRGEVCQHVFEEPAVDC
                     GRALAAWSDSRSGKRKGRRIGFPRFKKKIGETPSFRLRNKHPKGRQPAIRVGDHDRPR
                     SITLPGIGQVAVHDDTRRLRRMLANGRAKILFATVSHHAGRWWVSLNVEAASLHPARQ
                     HPVRDPGDDGGWVGIDRGLSSPPTQTAAK"
     gene            complement(5471..5704)
                     /locus_tag="VFC01_21395"
     CDS             complement(5471..5704)
                     /locus_tag="VFC01_21395"
                     /inference="COORDINATES: protein motif:HMM:NF023742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82217.1"
                     /translation="MTRHTKFKFCLDPTVQQQQVLARHAGAARFAFNQCLRMVKNALT
                     RDQTDSPAKVPWSGFDLINAFNGWKRPRTGVVW"
     gene            complement(5938..6918)
                     /locus_tag="VFC01_21400"
     CDS             complement(5938..6918)
                     /locus_tag="VFC01_21400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZN82218.1"
                     /translation="MKVMTALFGPTDAVERAHVLREAGASGVFTFEGPHDVFTPLALA
                     AAVEDLELMTNVAIAFPRNPIHLAHQANDLQLLTKGRFTLGLGTQVRTQIEKRFGAEF
                     DRPVARMTELVRALRAIFAAWNSGDRLDFRGEYYRHTLMTPTFSPGVNPYGPPPIYVG
                     ALGPRLTRAAARTADGLLVMPFGSKRFLREATMPAVREGLAAAGRDEGEFAVVPEIIV
                     STGDDHAATRRLLAFYGSTPAYRAVLDVHGWGDLQPELNRLSKQGRWEDMGRLIDDDM
                     LHTIAACGSPGEIAAHIRDRVAGVSDRICIYQPGPIAVESLAAIVDALNG"
     gene            6938..8290
                     /locus_tag="VFC01_21405"
     CDS             6938..8290
                     /locus_tag="VFC01_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HZN82219.1"
                     /translation="MSISPNADVVVVGAGFAGLSAARDLVRLGHDVVVLEGRDRVGGR
                     SSTTTIADTPVDLGGTFVGPTQDAVIALAKELGCETVRTHSRGKNLIRWRGKVRSYRS
                     TIPKLSMIELFDVSRIQWRFERVCRRVPVDEPWTSSIADILDSKTLDQWLRYVHAGAS
                     TRDLMAIMARVTWGCEPEEVSMLHAVRYIKAAGGLGRMLDVEGGAQQDRFPGGTQQIA
                     LRMAEDLGARVVLNAVVHSIDRHADGTLTVGSDRGDVAAKAVIIAVPPAHRAGIAFDP
                     ALPPEYEKLTEHWPQGNLSKAYAAYETPFWRENGCSGEALSDEGPVFITFDVSPSDAG
                     PGILLGFTDARTFDQLPAEQRRQQVLNGLAALFGDAARNPIDYVDHSWSTEQFAPGGP
                     TAAVPPGSWTTYGPWLRKPVDGIFWAGTETADEWTGFFDGAVRSGKRAADEVHRALAS
                     "
     gene            complement(8287..9786)
                     /locus_tag="VFC01_21410"
     CDS             complement(8287..9786)
                     /locus_tag="VFC01_21410"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HZN82220.1"
                     /translation="MILRQVLDHPVLQPARPVLLSGDDHVDHAVRWVHTADLYDIAPL
                     LRGDEILLTNGVGLIGVDETGRRLYVRRLAESGVAGLLFEVGRTFADVPADMVDEARV
                     VGLPVVELQPVLRFTEVAEAINSELIDRSVARLRHADEISRTLSEALARGATLGELIE
                     QIGTALGTWVKLSDYAGRVVVAAGEVDPNDADPSAEAPVLVEGTAWGRLTVGAAGVAE
                     VLVEAVMDRAPTVLGLCLIREQKDVAGALRAQQLLLDQLATNQSVDRGLLETRMRAAG
                     VATSDHEYACVAVDPRRVESAAKVVDGLIRQAGHGVFGLVEGTLCALVAGQTDETSPN
                     LAHVVRSAARDVLPRQARLCAVVGRAVRDPSDLPRAMSEARATLSLGQDLHLTQPIVG
                     VQALALERMLAAHGNLDATRRFVEDQIGVLQRADSAKSGQLSHTLEVLAACGGNKADA
                     AKRLHMRRQSLYYRLDQIAKLLDVDLDDPKQLVTLAVAVTARRMLSAST"
     gene            9873..10805
                     /locus_tag="VFC01_21415"
     CDS             9873..10805
                     /locus_tag="VFC01_21415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN82221.1"
                     /translation="MSTLVTGGRGFVGRHLVDQLLADGAKVVSYNRDFAVDSRDGLTV
                     VQGELFDIPRLTETLRRHSVDRIIHTAGQSHPGVSIELPWTTFKANAEGTLAVYEAAR
                     AAGVRRIVNFSSECALGSFDPGAPVDETVTPRPTTPYGVTKVTGELFGAVYNSLYGME
                     VVSLRVTEVYGPGLWMPSLLGDMIRAGLRGEPFHLHAGGDHPFQFVYVADVATAARLA
                     ASTEALTQPVYHVSGDRQITVAETARLLAGLIPGSRYDIGPGFLPDWDRQGPYDLTAS
                     TRDLGYTPAWKLERGLESQIDWLRSQEDSHARHD"
     gene            10798..11586
                     /locus_tag="VFC01_21420"
     CDS             10798..11586
                     /locus_tag="VFC01_21420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN82222.1"
                     /translation="MTNRIALVTGAASGIGLATAEALAREGADVALLSRPDDDLDIAQ
                     DRVKQHGGRVVTVAADVADPAAVRHAFERVEAELGPVDAVHNNAGISVACPLTETVDA
                     VFRRLVDTNLAGSFYVLREAARVMQPRRAGAIVNTASELAIIGQTGYVAYTATKGAIL
                     AMTRSAAAELSSWSIRVNAVCPGTTKTPMFLAEFDGVDDPAAELADNAASVAMQRIAE
                     PAEIAEAVVFLLSHRAGYITGTHLIADGGRTGCVPAGSIGVATK"
     gene            11612..13030
                     /locus_tag="VFC01_21425"
     CDS             11612..13030
                     /locus_tag="VFC01_21425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN82223.1"
                     /translation="MTEPAPTRLEQLGYSQQLERRLNIPEVVGLAMADVSPTMAVLFL
                     SAGVFFVGGTFSIGAGLILSVVVVFIALCLGELAGMFPIAGGMYSLVRRVLPGPLSWI
                     TMFNYLIQGVVIPASIALGMVGFLRDLFPGLGFPDTVIAIVLLALATGLALTKVEIGA
                     WATAAMVVVECVVLGIITVAALLHPHQQLSEVTFHPTVLSNGELVAVGFAVTLATLAP
                     AFNVINGYDAALGFSEELKGGERNIAKAVIISAVLACVLIMVPLIAAVIAAPDLKEFF
                     NAPAPVIYSVEASLGDKARIIIDIGVTIALFNAMLSLLMYFGRGVYTTGRDGVWPASV
                     NRIIGSINRFRAPGAGVMVLAIPAGVLVFTSALNFLIIFAGTVIAAVYFCIGMAALWS
                     RRSMAGEPRPYKMPLWPLPPLVVIGFTAIALATQERQYLVAEVVLICLALAAWAGSKK
                     WSASRVDAATVEPEMAKAVEAE"
     gene            13041..13862
                     /locus_tag="VFC01_21430"
     CDS             13041..13862
                     /locus_tag="VFC01_21430"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047789 - creatininase activity [Evidence
                     IEA];
                     GO_process: GO:0046449 - creatinine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HZN82224.1"
                     /translation="MTAHSAPRVYRHMTWEEIAAAAQADTPIVIPVGATEQHGRHLPV
                     CTDWVLPERVIDEASRVRDLIVGPFIPFGYRSRPGSGGGQHFPGTVSLRATTFMSVLE
                     DVLTELKRTGFRSIALYNWHFENSGFVYEPAYLVSEQNPELKIVVIEDVFPEFTAERR
                     TVIWPDEFPGLALEHAAVIETSLWMHFDPAAVRADRMAPDAPQRVVNHDVVPIDTRMS
                     TPSGSLSSPVSASPEKGRLITEWLVDRLIGILDEEFPNRAAPAADRNGERESSRA"
     gene            13859..14518
                     /locus_tag="VFC01_21435"
     CDS             13859..14518
                     /locus_tag="VFC01_21435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZN82225.1"
                     /translation="MKDSPATSRVLFVTASPREESHSVQLAEVFLDTYRNALPNVEVD
                     RLNAFTDLPAFTAEHVSAKVAVIAREAVPQTASGGWAEVLEVAARVQAADTLLFAVPM
                     WNGGIPWSLKLFVDIVTQPGVAFSFDPATGYTGLLGGRRAVEAYTSRVYAPHVPPAFG
                     VDHQSSYFNWWLKYCGIEDVHELRLQPTFPGEKFTAERDRVTAQAHALARTLAGQEVA
                     R"
     gene            complement(14500..15468)
                     /locus_tag="VFC01_21440"
     CDS             complement(14500..15468)
                     /locus_tag="VFC01_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN82226.1"
                     /translation="MGRGQEAGSPARKFAAAEALPKGRTVRVRSKDGIRLHAEVFGPE
                     DGYPIVLAHGITCAIRVWAYQIAELSTDYRVIAFDHRGHGRSAVPVRRHFYGLDHLAA
                     DLDSVLEATLAPGERAVIAGHSMGGIAITSWAERYPDRVPQRADAVALINTTTGDLLR
                     HVQFLPVPPPFADARVRAAGTLLKTFGAAPLLRAVDRPSRQFVSTIAVGRDADPAVVE
                     FIYELFTSTPPAGRGGWARTLVDALGPEHIGLTNLTVPTLVIGSEKDRLLPMVSSRRI
                     ASMVPNLASFVELSGGHCAIVERPDEVNKHLRMLIESVTEDQRATS"
     gene            15558..16532
                     /locus_tag="VFC01_21445"
     CDS             15558..16532
                     /locus_tag="VFC01_21445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HZN82227.1"
                     /translation="MRAIQIARLDGPQAAEVVDIEEPSAGDGEVLIDVHAAGVAFPDA
                     LQSRGLYQYKPEMPYTPGAEVAGVVRSAPDGAHVKAGDRVAGLTMLCGAMAEVVALQP
                     DRVFKLPDTVSFEAGAGLLFNDLTVHHALRTRGRLAEGESVLVHGAAGGVGTSALRLA
                     PAWGAGRTIAVVSTDDKIAAAKAAGASDVVLADGFKDAVKELTGGRGVDIVVDPVGGD
                     RFTDSLRSLAPGGRLLVIGFTGGDIPTVKVNRLLLNNVDAVGVGWGAWSLTHPGYLGE
                     QWAELEPLLASGKVSAPEPIVYPLERAAEAIASLEDRTAKGKVVVKLR"
     gene            complement(16593..17054)
                     /locus_tag="VFC01_21450"
     CDS             complement(16593..17054)
                     /locus_tag="VFC01_21450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82228.1"
                     /translation="MLGTTWYVTPAERAARLPCDELMPDAVRLDRAISIAASTAIVFA
                     WLCQLRIAPYSYDLLDNTGRRSPRIRCAVLTDLAVGQPFVRIFGRSYVFELASFAHDE
                     HITLRPHKVSVMSRFGSVSNTYAVRPDGDGTRLHVRVLFDGPWLVARRWRW"
     gene            17144..18013
                     /locus_tag="VFC01_21455"
     CDS             17144..18013
                     /locus_tag="VFC01_21455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HZN82229.1"
                     /translation="MVKRVMIAGALVMAPTLGVSGCATGNQSANETTTSSSPQTSASL
                     APGTERLSTQLKSADGTPVANATLDFANGYATVTVETVAAGILTPGFHGLHIHSVGTC
                     EANSVAPTGGASGNFNSAGGHFQAPGHTGHPASGDLTSLEVRSDGSALLTTTTDAFTA
                     ADLLAGGKTALIIHEKPDNFGNIPADRYQQKGGAAGPDEATLSTGDSGGRVACGVIES
                     ASATTTSSTTTTTTPSTTVTEAPVTTTSPATTPPPTTGTSTVTVTVPTTTTVPTSPPS
                     NPMQPGTTTPPGG"
     gene            complement(18017..19156)
                     /locus_tag="VFC01_21460"
     CDS             complement(18017..19156)
                     /locus_tag="VFC01_21460"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016881986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HZN82230.1"
                     /translation="MDGGPMGGRVVIAPDKFKGSLTAAEAADAISTGLKRGDPTLTTV
                     LCPVADGGDGTLGTVLAAGFERIPVYAKGPTGHVVHTAYARSGKTAVVEMAEICGLQR
                     LPGAVPAPMTATSHGLGAVVAQALDHGCRDIIIGVGGSASTDGGAGFLTALGATVRDQ
                     YGARIEPSGQNIGLAAQLDLTGLHPDIACASFTLASDVDNPLYGPLGAASVYAPQKGA
                     DPDEVIELDRALRRWADVVEHETRTDHRTAAGAGAAGGVGFAALAVLGARMQPGIDVI
                     LDRIELDKHLQDACAVVTGEGSLDRQSLRGKAAVGVCRRAGSYRVPTFAVAGVSALTA
                     DEARAAGFAGLYALSDVEPDRERSISHAAELLATVAEKLAQGLSA"
     gene            19460..20296
                     /locus_tag="VFC01_21465"
     CDS             19460..20296
                     /locus_tag="VFC01_21465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZN82231.1"
                     /translation="MTAPYTVNCSILLTDLPLLDRPGAARNAGFEAVEFWWPFDSPTP
                     PDADVNAFIRAIQDAGVQLTGLNFAAGDMPAGDRGILSDPALTQAFRDNVEIAVGIAE
                     TLGTRAFNALYGNRIDDISPATQDHVAAENLAHAARAADRIGATVLVEPVSGAPRYPL
                     LTAADAIRVIDRVHKEHGATNVRLLADFYHLHVNGDDITAAINDYGSRIGHVQIADAP
                     GRGEPGTGEIPIRTYLEQLEAQGYRGYVGLEYKATKPDTFEWLPRAERGATLQPHAET
                     RI"
     gene            20296..21183
                     /locus_tag="VFC01_21470"
     CDS             20296..21183
                     /locus_tag="VFC01_21470"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HZN82232.1"
                     /translation="MSTIAFIGLGIMGSPMARHLIKAGHTVIGLDTSQERIAAVVEAG
                     GKAADSIEQAVKGADVVAIMVPDSPQVQSVLLGDDGVFAHAQPGTLVIDFSSIRPDVT
                     AEFAKIGAERGLRILDAPVSGGEPGAVNATLSIMVGGSEADFATAKPIFDTVGKTIVH
                     VGANGAGQTVKAANQLIVAGNIELLAEAITFLKAYDVDIDAAVKVLGGGLAGSAVLDQ
                     KAGKMLARNFDPGFRIALHHKDMGIVTAAAREAGVVTPLGSLVAQLMASAMANGEGGL
                     DHSGLLLGVERLSGKETAQ"
     gene            21180..22979
                     /gene="gcl"
                     /locus_tag="VFC01_21475"
     CDS             21180..22979
                     /gene="gcl"
                     /locus_tag="VFC01_21475"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011595288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HZN82233.1"
                     /translation="MTRMRTVDAAVRILEIEGATQAFGLPGAAINPFYSAMRAHGGIK
                     HVLARHVEAASHMAEGFTRAAPGNIGVCIGTSGPAGTDMITGLYSASADSIPILAITG
                     QAPVDRLHKEDFQAVDIAAIAEPVTKMAMTVLEPGQVPGAFAQAFHLMRSGRPGPVLI
                     DLPIDVQLAEIDFDPEAYQPLPVYKPAASRAQAVKAIDMLVSAERPLIVAGGGIINAD
                     ASDLLVELAEILNVPVVPTLMGWGTIPDDHGLAAGMVGLQTSHRYGNATMLASDFVLG
                     VGNRWANRHTGGLDTYRRGRRFVHVDIEPTQIGRVFAPDFGIVSDAKAALEQFVAVAK
                     ERRAAGTLPDWSAWVEECQQRKRTMHRKTHFDDVPIKPQRVYEEMNRVFGRDVRYVTA
                     IGLSQIAGGQFLQVYGPRNWINCGQAGPLGWTVPAALGVVAADPDATVVGLSGDYDFQ
                     FLIEELAVGAQFNLPYVHVVVNNSYLGLIRQAQRNFDMNYFVSLGFDNINAQETDDSD
                     VPKGYGVDHLRVVEGFGCKAIQVVEPQHIGPALTRAQQLAREHKVPVVVEVFLEKITN
                     IAMGTEIDNIVEFNDLAETLEDAPTAEALLLLD"
     gene            complement(22976..23728)
                     /locus_tag="VFC01_21480"
     CDS             complement(22976..23728)
                     /locus_tag="VFC01_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009475272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN82234.1"
                     /translation="MADGVQSVERAFELLEIMASSGGSIALGELAARAELPQPTIHRL
                     VRTLLVMGYVRQLENRRYALGPKLIRLGESAAQLIGAWARPHLTELVERTGESANMAV
                     LDNDMAVYVAQVPSPHAMRMFTEVGRRVHLHCTGVGKALLMQLTNDNVHALLTRAGMP
                     ASNENSYTTPEALIRDIELSRSRGYAVDEGEQEIGVRCFAVPVPDSPTLTAISISGPA
                     ARLTLKSATKVTPLLKRVARDIASEFDKEAAV"
     gene            23920..24648
                     /locus_tag="VFC01_21485"
     CDS             23920..24648
                     /locus_tag="VFC01_21485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN82235.1"
                     /translation="MTQPLIRPRGTGLSAATTRTGPPPVERPAPLREAVYDALVEMII
                     TAELQPGQHLVENDLAAHLGVSRQPVREALQRMQTEGWVDLRPALGAFVHVPTEDEAD
                     QLLAARTLLETESARLAAEHATPAQVEHLWELHRAGEAALAADDQEGLVAANASLHSH
                     IVAMSGNKVLADLIGSVDRRVRWYYLPIARARGKDAWDEHAALITAIANGDGVRTGNL
                     MRRHTERTRETYHQRRRKAAEETT"
     gene            complement(24674..26059)
                     /locus_tag="VFC01_21490"
     CDS             complement(24674..26059)
                     /locus_tag="VFC01_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HZN82236.1"
                     /translation="MSSTFASYREVIDANGRVYRVGETDRELLGRSRAWMIWLPWMAM
                     MAVSVYEYGYGAAAKSIRDAHHWSMGETFWLLSIWAFFQAGVAFPAGKLREKGILSPR
                     AAMLIGAVLSALGFASITQGNLVLAYLGFAVCGGIGAGLVYATCINLVGKWYPERRGG
                     KTGFVNGGFAYGAVPFIFIFSYALHPNTYVWVLDLVAAYMLIVCAVCGWLFRDPPKNW
                     WPADVDPVKWADSKNGAASLKKNPPAVRQYTPMEAIKTGMLPLMWLSLGISAGVSLFG
                     ISYMVPFAKDLGFGPLIAASSAGVLSIINGTGRTVTGWLSDRFGRKQTLLVVLLIEAL
                     SLVGLLYAGKAESEIAFLAFAFLVGFGGGAFYPMFASLTPDYFGENNNASNYGLVYSS
                     KLVGSIVGIGVGASVIDAWGYTGAYWIAAASALVSAGIATFLRQPGRREPGPKSSAST
                     TQESLAPTLAD"
     gene            complement(26216..27484)
                     /gene="frc"
                     /locus_tag="VFC01_21495"
     CDS             complement(26216..27484)
                     /gene="frc"
                     /locus_tag="VFC01_21495"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HZN82237.1"
                     /translation="MVQIPTVVESDPPTGKALAGVRVLDMTHVQSGPSATQLLAWLGA
                     DVIKLEAPKTGDVTRGQLRDVPGADSLYFTMLNCNKRSITLNMKSDDGKRIFTDLVSR
                     VDILVENFGPGVVDRFGFTWDRLREINPRLIYASIKGFGPGRYFDFKAYEVIAQAMGG
                     SMATTGFEDGPPVATGAQIGDSGTGIHLVSGILAALYQRTNTGRGQRVEVAMQEAVLN
                     LCRVKLRDQQRLASGPLHEYPNERFGDEVPRSGNASGGGQPGWAVKTKGGGPNDYIYV
                     IVQPQGWASLAELIGRLDLAEHPDWARPEDRLPKLDKVFALIEDWSERHTKWEALEAL
                     NALNIPCGPVLSTKELIEDETLAELGAIVTVDHPERGPFKTVGSPLRLSDSPVDIVRS
                     PLLGEHTDEICTELGYLPEQLELFRSAGVI"
     gene            28048..29274
                     /gene="frc"
                     /locus_tag="VFC01_21500"
     CDS             28048..29274
                     /gene="frc"
                     /locus_tag="VFC01_21500"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HZN82238.1"
                     /translation="MGKALEGVRVLDMTHVQSGPSCTQILAWLGADVVKLEAPSGDVT
                     RKQLRDIPGADSLYFTMLNCNKRSITLNMKSPEGKRIFTELLPRFDILAENFGPGAIE
                     RMGFGWDELQRINPRLIFASIKGFGDGPYTHYKAYEVIAQAMGGSMSTTGFEDGPPLA
                     TGAQIGDSGTGIHTVAGILAALYQREHTGRGQRVTVAMQHAVLNLCRVKLRDHQRLEH
                     GPLGEYPNEDFDDCVPRSGNASGGGQPGWAVKCAPGGPNDYIYVVVQPVGWEPIANLI
                     GRPELVTDPEWSTPQARLNKLDKMFQLIEEWTMGLGKWQALELLTEHNIPCGPILSTR
                     EIVDDTSLAANEMVVRVDHPERGEFTTVGCPIKLSDSPVDIDRSPLLGEHNAHIYGDE
                     LGLGDQLVALKAKGVI"
     gene            29340..30731
                     /locus_tag="VFC01_21505"
     CDS             29340..30731
                     /locus_tag="VFC01_21505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HZN82239.1"
                     /translation="MNASAQPAYREVVDGNGRVYRVGETDRDILGRSRAWMVWLPWIA
                     MMAISSSEYAFTSAEDTLSEAHGWHGAHIFWLLGVWVLFQAAVAFPAGRLRESGRLSA
                     RSAMLLGAAGALLGYLSLAYAPHVLLAYIGFGFFGGGGAGFVYATCINMVGKWYPERK
                     GGKTGFVNGGFAYGSVPFIFLFTNYMDLENYEGILLAVGIFLAGLVAATGFFFVDPPK
                     NWWPPHVDPLKVNDDPRIRRALRKNPPAVKQYTPMEAVKTGILPLMWFVLLCTAGINI
                     FGIAMQVPFGKEMGFAGGIVALAMSLKAIINGTGRGVIGWISDRVGRRDTLIIVCVVL
                     GLAQYAVYFSGSIGSMPLFLLASMISGFGGGAIFPLFAAMTADYFGENNNATNYGLVY
                     SSKLISGLVGAGLGAVVVDTWGYGAAFGIAGSVGIACAFISVLLRQPKLKESTPTRPT
                     EPTEAGARVLVVD"
     gene            30913..31632
                     /locus_tag="VFC01_21510"
     CDS             30913..31632
                     /locus_tag="VFC01_21510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN82240.1"
                     /translation="MSLPTPSPRRRGPTLADAAARRVQRPAPLRQAVYDAIVDLIVQG
                     TLQRGQHLVENELAEYLGVSRQPVREALQRLQTDGWVDLRPAQGAFVHTPSEDEVDQL
                     LGVRTVLETYSARLAAENATPEDVNRLWELQQDGLDALAANDSEALVKANVNLHAEIT
                     AVAGNSVLAKHIELVERRWHWYYLPIAQPRGQGAWAEHAELLKAIAAGDADLASSIMH
                     RHTEHTRQVYHDQRQTKSDAE"
     gene            complement(31642..32484)
                     /gene="fdhD"
                     /locus_tag="VFC01_21515"
     CDS             complement(31642..32484)
                     /gene="fdhD"
                     /locus_tag="VFC01_21515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZN82241.1"
                     /translation="MGRVTSRYRVVRMADGVATARPDSLAAEEPLEIRVAGRPLTVTM
                     RTPGNDFDLARGFLVSEGVVAADSDIAGIRYCAGATADGVNTYNVLDVLLADGVPLPD
                     VSVERNFYTTSSCGLCGKASLEAVRTISRWRVARDPLRLNTETIAALPDKLRAAQRVF
                     DRTGGLHAAALFDAAGELWCVREDVGRHNAVDKVIGWALDQGRLPLSGMTLMVSGRAS
                     FELVQKAVMAGIPALAAVSAPSSLAVDLARETGLTLIGFLRGTSMNVYSGVERIPSGE
                     PLPC"
     gene            32571..33920
                     /locus_tag="VFC01_21520"
     CDS             32571..33920
                     /locus_tag="VFC01_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HZN82242.1"
                     /translation="MSPTSAPVAVVEVRDLHGRSYRVGERPHDIIGHSRKWMLGLSWA
                     AMAGIGVLQYGYGVAVSALHATDGASVTGALWVLALWVVFQAGAAAPTAALSHRFALA
                     PSRAVLVGALLCAAGPLTLAYSDSLALAVLGYSVLCGTGAGIVYATCTSTVAKWYPEE
                     RAVRVGFVTGAFGYGAVPFIALFVGVLTPGNQTEVFAGVGVCVLLVVAVCGALLRDPP
                     AGWWPPEIDPKLWAIDKRVNRSLRNNVPAVRQYSPREAVRTPAFVVMYLILVFAAAAS
                     LLAIVYVPFVAMSHGFSLAIGASAVGLLAVVNGAGRSVAGRISDRFGRRQTLSAALLL
                     EGCAQLGLVYSASVGQPAAFVVFAALSGMAGGAFYPLFASLVADYFGERNAVRNFGVV
                     YSAKLFGGIIGVGLPALVVASDGLTLLFVAAGLLSLCAAVMTRLLHRPGYPALHLPR"
     gene            33945..34271
                     /locus_tag="VFC01_21525"
     CDS             33945..34271
                     /locus_tag="VFC01_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82243.1"
                     /translation="MSSTAEREELNELSGALLKCVTSLASKCGDTPAMRRLVNDAERI
                     LNGVDRLDIDVEELELTRGHTPSVEMIQIPDTQYDIDFWRDVDHEGIGGQNGVCVPVS
                     SGRKRR"
     gene            complement(34494..34628)
                     /locus_tag="VFC01_21530"
                     /pseudo
     CDS             complement(34494..34628)
                     /locus_tag="VFC01_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008714153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(34721..>35193)
                     /locus_tag="VFC01_21535"
     CDS             complement(34721..>35193)
                     /locus_tag="VFC01_21535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HZN82244.1"
                     /translation="PTTLSAALGGSAAVVVLYRGAWCPYCNLALRAYQDDLVPTLANK
                     GVALVAISPQKSDGSLSAQEKNALTFIVLSDPGNKIAKRLGVMTAPPDDAQAAQRAMG
                     LDLPEVNADGTYSIPMPTVVIVDPTGVIRWIDVHPNYTTRTEVSDILAALDTVS"
BASE COUNT         6149 a        11572 c        11436 g         6036 t
ORIGIN      
        1 ctactcaggt gtcagcgatg tcccgagaca tacaactgtc agcgatgtcc cgaaacagga
       61 cagtacgctc agcgacggtt agcgcgccga aatcgcaaat agtgccgccg cggccatcgc
      121 ctcgacgacg cagtagaacc agttcgggta gaacgccgtg cggtcgccta ggaccgcggc
      181 cacgagacgg ccgaatgcca tacctgccaa cgcggcgccg acggcgatca ggatgccctt
      241 ccggaaatca ccgtccgcga cggccgcatg aaccaacaca cccgcgatcg cgagaccgaa
      301 gcctccgtac acggcacgca cctccgagcg tgacgccgac tcccccagcg tgatgccgaa
      361 cggacgcacg agcgccgcgg gcgctgccag cgcatagacg cccatgccga ggaagaacac
      421 ggcgataacg ccgatgaccg cgaccgccac ggtagcctcc tgtcgaattg aggtaggagg
      481 ccagcatgac ggcaaaggcc gctcccgcgc ttccacaaac ctgctgtccg tcggtgtggc
      541 tctggcccgg ccaagccctg tacgcaggcc cgagcctcaa cctcgcaccg cattcgggct
      601 ccgtgtggtg cctcgcggtc ggcatcgacg gggcactctc cgtctcgacc aaagacggca
      661 tcaagcgcac cgcgcacagc gtgctgatac ccccgcggct gactcaccag ctgacctgcc
      721 acggcgcggg cctcgtctct tgctatctgg aaccgacatc ggagcgcgcc gacaccggcc
      781 gacacaagtt cggcgaatgg cacggtgagg tcgctgtcag acacgtggac gaagagcgat
      841 tgcagttcac cccgaccgac gacgaaagcg cctcgcactg gctcgatttg gcggcccccg
      901 cggcaccacg tcaggtcgat ccgcgtatcg ccgcagccgc ccgcagaatc cgtgaagatc
      961 cggcggcggc catttcctca cgagagctgg ccgcacaggc aggactgtcc gaatcgcggt
     1021 tcctgcacct gttccgcgac gagctcggca ccagtgtgcg ccgctaccga ttgtgggccc
     1081 ggttgacgca tgcgggtgcg gccatcgcgg cgggcgacaa cttgaccacg gcggcgatga
     1141 aggcgggttt cgccagcccg tctcacctcg ccgaccggtt caagacgacg ttcggcttgt
     1201 ccgctagccg actgctcggg acggggctga ccctgcgggc tccgtagccg cgcggccgaa
     1261 caccatcgac tcctcgatgg attcgaacgg ccggccgacc acgtctcgta ggaagttgtg
     1321 cgacatcagc cacggcctgc gagtgcccga cttcggcagc aggtgcagcc ctcgcttcac
     1381 gtagcccgag tccaggttga acgccggctg ctcgggcatg tcgacgttac cgagatgtgc
     1441 atacgcacgc gtgtatccat gagaatccat gtacgccaat aacttcgcaa acgactgcgc
     1501 ggtcaggtcg gcccccaacg tccacgacgc atttgtgtag ccgacgcacc aggccgcatt
     1561 gggaacgtcc tccagcatgt ggcggcgata caggaaccgg tcgtgcgggt ccaccttctg
     1621 cccatcgaca gtgatcgtcg cgccacccaa ggactgcaac tgcaggcccg tcgcggtgac
     1681 gatgacgtcg gcgtccagac ggcgaccaga gcgcaacacg atgccggagg cgtccatgtg
     1741 atcgatgtgg tcggtcacca tctcgaccct gcccttggcg atttccttgt acaggtcggc
     1801 accaatgatg aagcacagcc gctgatccca cgggttgtac ggcggcgtga aatgcgtatc
     1861 tatgtcgtaa cccgcaggca gagtgtgctc ggcgatgcgc cgcagaaaac gcttgctgaa
     1921 ctccggcgcc cggcgggcaa ccagccacag gatggcgccg atgaccgcgt tgcgccaccg
     1981 cacgatccag tgggcaacct tgagcggcag cactttccgt atcgcgagag cggtcggctc
     2041 gatcttcgtc gcggagatca tgtacgacgg cgtacgctgc aacatcgtca cgtgagcggc
     2101 cgtgtctgtc agcgatggaa taagactgac cgccgtcgct ccgctgccga tgaccacgat
     2161 tcgcttgccc tcatactcga acgattcggg ccagagctgc ggatgcacca cctcgccctc
     2221 gaagtcttcg atgccgggaa atgttggcgc atagggctcg tcgtagttgt aatacccggt
     2281 gccgaagaac aggaaccggc tctgatacgt cgcaagctgg ccgtcctgtt cggcgcgcac
     2341 cgtccaggtg tcggtcgtcg aatcccaatc cgcggaccgc acataggagt ggaacctgat
     2401 gtgcttgtcg atgccgtact tgcgcgcggt ctgatcgagg tactcccaga tgtgttcccc
     2461 gtcgacgatc atctccgggc gagtccatgg ctcccaggga aagcacagcg tgtagatgtc
     2521 gctatccgac cgaatccccg gataccggaa caggtcccac gttccgccga cccgcgcgcg
     2581 tcgctccaga atcgtgtagg acagccgcgg gttgcgctgg cggatgcggt acgcggcgcc
     2641 gatgccggag atccccgcgc cgatgatcag cacgtccgag tattcaggct cgctcaccgg
     2701 taaacctcct aggcgggctc gacctccctg gcttcggcgc ggccgaacac catcgactcc
     2761 tcgatgcggt cgaagcgatg gtcaatggcg tcgagcacgt aattgtgccg tacattccac
     2821 ggccggtgcg tcccggactt cggcagggca tgcgcggcac gctgcacata accggcgttg
     2881 atgttccacg ccggcttctc cggcatcggc ttgtcaccga ggtgcgggta cgcgtgcgta
     2941 tacccatggg agcccatgta ggccaacagc tttgccaccg cgcgcgccgt cagatccgcc
     3001 cgcagcgtcc acgatgcgtt gatatagccg acgcaccacg ccaggttcgg gatgtcttcg
     3061 agcatgtgct ccttgtagac gaaccggtcc tgcggcttga cctcggcgcc gtcgatgctc
     3121 aacgacaccc cgcccagcgc ctgcagctgc aggccggtcg ccgtgatgat cacgtcggcg
     3181 tcgacatggg gtccggactt caggacgatt ccgctgctgt cgatgtggtc gatggtgtcg
     3241 gtgacgacgt cggcacgccc gtcgccgacg gcggcgtaga ggtcgttgtc gaggatcagg
     3301 cacaatcgct ggtcccatgg gtcatagggg ggcctgaagt ggatatcgac cggatagtcc
     3361 tcgggcaggc tctttttcgc catcgcccgg atcagccatc tgctgaacct gggcgccttg
     3421 cgggcgacca cgtaggtcat cacggtgaag aacgtgttgt acagccggac ggcgtggttg
     3481 gcggcccgca tcggcaacac ttttcggatc gcctgcacca cggggtggat tcgaggcatc
     3541 gacaacatgt aggtgggcgt gcgctgcagc atggtcacgt gtccggcctg gtcggtcagc
     3601 gacggaatca tgctgaccgc cgttgccccg ctgccgatca ccacaacacg tttgccggtg
     3661 tagtcgaagc cctcgggcca gaattgaggg tgcacgacct cgccggtgaa ggtctcgatg
     3721 ccggggaact ccggccggta gggctcgtcg tagttgtagt agccggtgcc gaagaacagg
     3781 aatcggccgc ggtagctctt gggcatcccg tcctgttcgg tctccaccgt ccaggtgtcg
     3841 gtggccgagt cccagtcggc ggagatgacg cgggtgttga acctgatgtg ctcgtcgatg
     3901 ccgttcttgc gggccgtctc ggtcagatac gcccggatgt caggcccgtc ggccacgttt
     3961 tcgggccggg tccacggctc gtagggaaag ctcagcgtga agatgtcgct gtctgagcgg
     4021 atgccgggat aacggaacaa atcccaggtg ccgccgatgc gctggcgccg ttccagcacc
     4081 gtataggaca gccccggatt cttctcgtgg atgcgatacg cggcgccgat ccctgaaatt
     4141 cctgcgccga tgatcagcac gtcggagtac tcggcatcgt gagcgcccac gacgacctcc
     4201 ttttggtccg cctcggtacc acgataaagc gtgtcgaaca actctggtgt gagtgtcggg
     4261 caccgccctt ctcgggcgtc cgtcggttca cgccgcgggt gcggtatgaa cgtcggttcc
     4321 cgcgtcaacc gggctggttt caccggcaca cgaagggtgc tggtcagcgc cgtcccgtcg
     4381 gcgggcgttg gtggcccggc ccccgtgctt gggggtccgg gggtcgaggt agtgcgtttg
     4441 accccaacag gccagattcg ttgcggcgtt gcggtcgcgg tcagcggaat gcccgcagcc
     4501 gcagttgaac acccgatcgg ccagagtcaa atcgtcgcga actgccccgc attgcgggca
     4561 aagcttgctc gagggatacc agcgatctgc gacggcaagc tcgccacccc gccaggcctg
     4621 tttgtaagcc aggattcgag cgaactcggc ccagccggca tctgagatgg cgcgtgccag
     4681 gtggtggttg cccagcatcc ctgtgacgtt caggttctcg atcacgagtc ggtcgtgggt
     4741 cttgaccaac tcattggaca cctggtgtag gaaatggcgg cgcacattgg cgacatggtg
     4801 gtgatgacgc cccagcttgg ctgcggcatc tttgcggttg cgggatcctt tctgcttgcg
     4861 cgacaacgat ttccctagcc gttgttgtcg cttcaatccg gcggcgagcg ccttgggggc
     4921 atcgttgata cgggctactt cgcggccgtc tgcgtcggcg gcgacgacaa accccggtcg
     4981 atacccaccc acccgccatc gtctcctgga tcccgcaccg gatgctggcg ggcagggtgc
     5041 agactggcgg cctccacgtt cagcgacacc caccaccgtc ccgcatgatg gctgacggtg
     5101 gcgaacaaaa tcttcgcgcg gccgttggcc agcatgcgtc gcaaccggcg ggtgtcatcg
     5161 tggacagcga cctgcccgat cccgggtagt gtgatcgagc ggggacgatc gtgatcgccg
     5221 acccggatcg ccggttggcg acccttggga tgcttgttgc gcaaccgaaa tgacggcgtc
     5281 tcgccgatct tcttcttgaa gcgtgggaag ccgatccgcc tgcctttgcg cttgcccgag
     5341 cgcgagtccg accaggccgc cagcgcgcgc ccgcagtcca cagccggttc ttcgaagacg
     5401 tgctggcaca cctcgcctcg ccactccaag ccggtcacca cgacctcggc gacgccgtcg
     5461 gcgtccacag ctaccacacg acccccgtcc tcggtctttt ccatccgttg aacgcattga
     5521 tgagatcaaa accgctccac ggcaccttcg caggcgagtc ggtctggtcg cgagtgagcg
     5581 cgtttttcac catccgcagg cactgattga acgcaaaccg ggcggccccg gcatgccggg
     5641 caagcacttg ctgctgctgc acggtggggt ccaggcagaa cttgaacttc gtgtgccgag
     5701 tcatttccgg cgagcatccc accacacacc gacaacacct gaccgggcgt gacgaatcag
     5761 gccgaatgat caccatcgat ggtggtcgaa cgcaccgacg gctggatcaa cgactgccgc
     5821 cgcttcgacc gcatcacgaa ggcaccctgc acgctcgcca agacgtgatt ctcggccacc
     5881 tccccttacg taaccgagta cgcgatcgat cccagctgtt cgaaacgctc taggggctca
     5941 gccgttgagc gcgtcaacga tcgccgccaa cgactccacc gcgatcggtc ccggctggta
     6001 gatgcagatc cggtcggaaa cacccgccac ccgatcacga atatgcgcgg cgatctcgcc
     6061 ggggctgccg caggccgcga tggtgtgcag catgtcgtcg tcgatgagcc ggcccatgtc
     6121 ctcccatcgg ccctgcttcg acagtctgtt gagctccggc tgcaggtcac cccagccgtg
     6181 cacgtccagc accgcgcggt acgcaggcgt cgagccgtag aaggccagca gccgacgagt
     6241 ggccgcgtga tcgtcgcccg tggacacgat gatctccggg actacggcga actcgccctc
     6301 gtcgcgtcca gcggcagcca acccctctcg caccgccggc atcgtcgcct cgcgcaggaa
     6361 ccgcttcgag ccgaacggca tgacgagcaa accatctgca gtcctggcgg cggcccgggt
     6421 cagccgcggg ccgagcgcgc cgacgtagat cggtggcggg ccgtacgggt tgacgccggg
     6481 actgaaggtc ggggtcatca gcgtgtgccg gtagtactcc ccgcggaagt cgagcctgtc
     6541 accggagttc cacgccgcga agatggcccg cagcgcacgc accagttccg tcattctggc
     6601 aacgggccgg tcgaactccg cgccgaaccg cttctcgatc tgcgttcgca cctgggtgcc
     6661 gaggccgagc gtgaatcgcc ctttggtcag caactgcaga tcgttggctt ggtgtgcgag
     6721 atgaattggg ttgcggggga acgcgatcgc gacgttcgtc atcagttcga ggtcctcgac
     6781 cgcagcggcc aaggccagcg gtgtgaacac gtcgtgcggt ccctcgaagg tgaagacgcc
     6841 gctggcgccg gcttcccgca gcacgtgcgc gcgttcaacg gcatcagtcg gtccgaacaa
     6901 cgctgtcatg accttcacgg cgtgagagcc tagtcacatg agcatttcac ccaatgccga
     6961 cgtcgtcgtc gtgggtgccg ggttcgcggg gctgtcggcg gcccgtgacc tggtgcggct
     7021 cgggcacgac gtcgttgtcc tggaaggccg cgatcgcgtc ggggggcgct cgtcgacgac
     7081 gacgatcgcg gacacaccgg tcgatctcgg cggaacgttc gtcggaccaa cacaggacgc
     7141 ggtcatcgcg ctcgcgaagg agctgggctg cgagaccgtg cggacgcaca gccgcggtaa
     7201 gaacctgatc cggtggcgcg gcaaggtccg gtcgtatcgc agcacgatcc ccaagctgtc
     7261 gatgatcgaa ttgttcgacg tgtcccgcat ccagtggcga ttcgaacggg tctgccggcg
     7321 ggtgccggtg gacgagccgt ggacatcgtc gatcgccgac atcctcgact ccaagacact
     7381 cgaccagtgg ctgcggtacg tgcacgcggg cgcgtcgacg cgggacctga tggcgatcat
     7441 ggcccgtgtg acgtggggct gtgaacccga ggaggtgtcg atgctgcacg cggtccgtta
     7501 catcaaggcc gcgggcgggc tcggtcgcat gctcgacgtc gagggtggcg cccagcagga
     7561 ccgcttcccc ggtggcacgc agcagatcgc gttgcggatg gccgaagacc tcggtgcgcg
     7621 tgtggttttg aacgcggtcg tgcacagcat cgaccggcat gccgacggca ccctgaccgt
     7681 cggttcggat cgcggtgacg tagcggcaaa ggccgtcatt attgccgtcc cacccgcgca
     7741 ccgagcaggg atcgcgttcg atccggcgct gccgcccgag tacgagaagc tcacggagca
     7801 ctggccgcag ggcaatctga gcaaggcgta tgccgcgtac gaaacaccgt tctggcgcga
     7861 gaacggctgc tctggtgagg cgctttccga tgaggggccg gtgttcatca cgttcgacgt
     7921 gagcccgagc gacgcagggc cgggcatttt gttgggcttc acggatgcgc gcacattcga
     7981 ccagctgcca gccgagcagc gccgtcagca ggtgctgaac ggattagcag cgctcttcgg
     8041 cgacgccgcc cggaatccga tcgactatgt cgatcacagc tggagcacag agcaattcgc
     8101 gccaggcggc ccgacggcag cggttccgcc gggctcatgg acgacgtacg ggccatggtt
     8161 gcgtaagcct gtcgacggca tcttctgggc gggcaccgaa accgccgacg aatggacagg
     8221 gttcttcgac ggcgcagtgc gatcaggcaa gcgcgccgcc gacgaggtgc accgagcgct
     8281 ggccagttag gtactcgccg acagcattcg tcgtgcggtg accgccaccg cgagcgtgac
     8341 gagttgtttc gggtcgtcga ggtcgacatc gagcagcttc gcgatctggt cgaggcggta
     8401 gtacagagac tgcctgcgca tatgcagacg tttggccgca tccgccttgt tgccaccgca
     8461 cgcggcaagc acctcaagag tgtgcgaaag ctggccgctc ttagcggaat ccgccctctg
     8521 gagcacacca atctggtcct ccacgaaccg gcgcgtggcg tcaaggtttc catgcgcggc
     8581 gagcatccgt tcgagtgcca acgcctgcac gccgacgatg ggctgtgtca ggtgcaggtc
     8641 ctggcccagg ctcagcgtgg cgcgcgcctc agacatcgcg cgcggcagat cggacgggtc
     8701 gcgcaccgcg cggccgacca cggcgcacag ccgggcttgc cgcggcaaca cgtcacgtgc
     8761 agcgctgcgg acgacgtgcg caagattcgg cgacgtctcg tcggtctgtc cggccaccaa
     8821 tgcgcacaac gttccctcga cgaggccgaa aaccccatgc cccgcttgcc gaatgagccc
     8881 gtcgacaacc ttggccgccg actcgacccg gcgcggatcc accgcgacac aagcgtattc
     8941 gtgatcggag gtggccactc ccgcggcgcg cattctggtc tcgagcaggc cgcgatccac
     9001 ggactgattg gtggcgagct gatcgagcag tagttgttga gcacgaagcg cgccggcgac
     9061 atccttttgt tcccgtatca gacacaggcc caaaaccgtt ggggcgcgat ccatgacggc
     9121 ttcaacgagc acctcggcga cgccggccgc gccgacggtc agccggcccc acgcggtgcc
     9181 ttcgacgagc acaggggcct cggcactcgg gtcggcgtcg ttcgggtcta cctcgcctgc
     9241 ggcgacaacg accctgcccg cgtagtcact gagcttcacc caggtcccca gcgcggtgcc
     9301 gatctgttcg atcagctcac ccagtgtggc gccgcgagcc aatgcctcgg acagcgtgcg
     9361 cgagatctcg tcggcatggc gcagtcgggc gaccgagcgg tcgatgagtt cactgttgat
     9421 ggcttcggcc acctcggtga agcgcagtac tggctgcagt tcaacgaccg gcaacccgac
     9481 gacgcgcgct tcgtcaacca tgtccgcagg gacgtctgca aaggttctgc cgacctcgaa
     9541 cagcaggccc gcgacgccac tctccgccag ccggcgcacg tagagccgcc gccccgtttc
     9601 gtcaaccccg atcagcccga cgccattggt cagcaggatc tcgtcgccgc gcagcagcgg
     9661 agcgatgtcg tagaggtccg cggtgtgcac ccaccgaacg gcgtggtcga cgtggtcgtc
     9721 gccgctgagc agcaccggcc gggcgggttg gagcaccggg tggtcgagaa cctgccggag
     9781 gatcacgtag gacagtatgt cccacaaacc tgggcaaagt tatgacattg cgtccgttga
     9841 tgcacgcaat gaccggtcgc atgctggcac ccatgagcac actcgtgaca ggcggccgcg
     9901 gattcgtcgg acgccatctg gtcgaccagc tgctggccga tggcgccaag gtcgtcagct
     9961 acaaccgcga tttcgccgtc gattcccgcg acgggctgac cgtcgtgcag ggcgagctgt
    10021 tcgacattcc gcggctcacc gaaacgctgc gccggcacag cgtcgaccgc atcatccaca
    10081 ccgcgggcca gagccacccg ggtgtgtcga tcgagttgcc gtggaccacc ttcaaggcca
    10141 acgctgaagg cacgctcgcc gtctatgagg cggcccgcgc ggcgggtgtg cgccggatcg
    10201 tcaacttctc gtcggagtgc gcactgggca gtttcgaccc tggcgcccct gtcgatgaga
    10261 ccgtcactcc tcgaccgacg actccatacg gcgtcaccaa ggtcacgggc gagttgttcg
    10321 gcgccgtcta caactcgttg tacggcatgg aggtcgtgtc gctgcgcgtc accgaggtct
    10381 acggccccgg gctgtggatg ccaagtctgc tcggcgacat gatccgtgcc gggttgcgcg
    10441 gcgaaccatt ccatctccat gcgggcggcg accatccctt ccaattcgtc tatgtcgccg
    10501 acgtcgccac cgcagccagg ttggccgcga gcaccgaggc gttgacacag cccgtctacc
    10561 acgtgtccgg cgaccgtcag atcaccgtcg ccgagacggc gcggctgctt gccggactca
    10621 ttccgggcag ccgttacgac atcgggcccg gcttcctgcc cgactgggac cgccaagggc
    10681 cctacgacct gaccgcgtcg acacgcgacc tcggatacac cccggcttgg aagttggagc
    10741 gcggacttga gagccaaatc gattggctgc gttcgcagga ggattcgcat gcccgacatg
    10801 actaatcgca tcgccctggt gaccggcgcc gcatccggaa tcgggctggc caccgccgaa
    10861 gcgctcgctc gcgaaggcgc cgacgtcgcg ttgttgtccc gaccggatga cgacctcgac
    10921 atcgcgcagg accgggtcaa acagcatggc ggccgcgtgg tcaccgtcgc cgccgacgtc
    10981 gcagacccgg ccgcggtgcg ccacgcgttc gagcgcgttg aggccgagtt gggacccgtt
    11041 gacgccgtgc acaacaacgc cggaatctcg gtcgcctgtc cgctcaccga gaccgtcgat
    11101 gccgtgtttc ggcggctggt ggacaccaac ctcgccggtt cgttctacgt gttgcgagag
    11161 gccgcgcgcg tcatgcaacc ccgccgcgca ggagcgatcg tcaatacggc gtcggaactg
    11221 gcgatcatcg ggcagacggg gtatgtcgcc tacaccgcga ccaaaggcgc gatcctggcg
    11281 atgacacgca gcgccgcagc cgagttgtcg tcatggagca tccgggtgaa cgcggtttgc
    11341 ccgggcacca ccaagacgcc gatgttcctc gccgagttcg acggtgttga cgaccctgcg
    11401 gccgaactcg cggacaacgc ggccagcgtc gcgatgcagc gcatcgccga acccgccgaa
    11461 atcgccgaag cggtggtgtt cttgctgtcg caccgcgccg gatacatcac gggcacacat
    11521 ttgatcgccg acggcgggcg caccggctgt gtgcccgccg ggtccatcgg cgtcgcaacc
    11581 aagtaaccgc caccaccacc ggagaaccac catgaccgaa cctgcaccaa cccggctcga
    11641 acagctcggc tattcacagc aactcgaacg cagactgaac atccccgagg tcgtcgggct
    11701 ggccatggcc gacgtctctc ccaccatggc agtgctattc ctttctgccg gagtgttttt
    11761 cgtcggtggg acgttctcca tcggcgcggg gctgattctg tcggtcgtcg tggtcttcat
    11821 cgccctgtgt ctgggtgaat tggccgggat gttcccgatc gccggcggca tgtattcgct
    11881 tgtgcggcgc gtcttgccgg ggccgctgtc gtggatcacg atgttcaact acctcatcca
    11941 gggtgtggtg atcccggcca gcatcgcgct gggcatggtc ggattcttgc gagacctgtt
    12001 ccccggtctt ggatttcccg acaccgtgat cgcgatcgtg ttgctggcgc ttgccaccgg
    12061 actggccctg accaaggtgg agatcggcgc atgggcgact gcagcgatgg tcgtcgtcga
    12121 atgcgtcgta ctcggcatca tcacggtggc ggcgctgctg catccgcatc aacaactgag
    12181 cgaggtcacc ttccacccga ccgtgctcag caacggcgaa ctggtggcag tcggcttcgc
    12241 ggtcacgttg gccacgctcg cgccggcatt caacgtgatc aacggctacg acgcagcgct
    12301 gggattctcc gaggaactca agggcgggga acgcaacatc gccaaggcgg tcatcatctc
    12361 cgccgtcctg gcgtgcgtgc tgatcatggt tccgctgatc gcggcggtca tcgccgctcc
    12421 tgacctgaag gagttcttca acgcacccgc cccggtcatc tactcggtcg aggcgtcact
    12481 gggagacaag gcccgcatca tcatcgacat cggcgtgacg atcgcgttgt tcaacgcgat
    12541 gctgtcgctg ttgatgtact tcggccgcgg cgtgtacacg accggacgcg acggtgtgtg
    12601 gcccgcgtcg gtgaaccgga tcatcggctc tatcaacagg ttccgcgcac cgggcgctgg
    12661 cgtcatggtg ctcgccatcc cggcaggcgt gctggtcttc acgtcggcgc tgaacttcct
    12721 catcatcttc gccggcacgg tgatcgccgc cgtgtacttc tgcatcggca tggccgcgct
    12781 gtggagccgg cgcagcatgg caggtgagcc aaggccgtac aagatgccgc tgtggccgct
    12841 gccaccgctg gtggtcatcg ggttcactgc gatcgcgctg gcaacccagg agcggcaata
    12901 ccttgtcgcc gaggtggtgc tgatctgtct ggctctcgct gcctgggcgg gatccaagaa
    12961 gtggtccgct tcccgcgtcg acgcggccac cgtcgaaccc gagatggcga aagccgttga
    13021 agcagaatag gagttcttcg atgacagcac attccgcgcc gcgcgtgtat cgacacatga
    13081 cgtgggagga gatcgccgcg gccgcgcagg ccgacacccc gatcgtcatt cccgtcggcg
    13141 ccacggagca gcacggacgt catcttccgg tatgcacgga ctgggtgttg ccagaacggg
    13201 tcatcgacga ggccagccgc gtccgcgacc tgatcgtcgg gccgttcata ccattcggct
    13261 accgcagtcg cccgggaagc ggtggaggcc agcacttccc cggcacagtc tcgctgcggg
    13321 caaccacatt catgagcgtg ctcgaggacg ttctgaccga gctcaagcgc accgggttcc
    13381 gcagcatcgc gctgtacaac tggcacttcg aaaacagcgg attcgtctac gagccggcct
    13441 atttggtctc cgaacagaat cccgagctca agatcgtcgt gatcgaagat gtctttcccg
    13501 aattcaccgc ggaacgccga accgtcatct ggcccgatga gttccccgga ctggcactcg
    13561 aacacgccgc ggtgatcgag acgtcgctgt ggatgcactt cgacccggcg gccgtgcgtg
    13621 ctgatcgaat ggcacccgac gctccgcaac gggtggtcaa ccacgacgtc gtccccatcg
    13681 acacgcgcat gtcgacgccg agcgggtcgc tgtcgtcacc ggtgagtgcc agtcccgaga
    13741 agggccggtt gatcaccgag tggctggtgg accgcctgat cggcatcctc gacgaggaat
    13801 tcccgaaccg agcggccccc gcggctgatc gcaatggcga acgcgaaagc tcaagggcgt
    13861 gaaggattca cccgcgacgt cgcgggtgct gttcgtcacc gcttcgccgc gcgaggaatc
    13921 gcattctgtg cagctcgccg aggttttcct ggacacctac cgcaacgccc tgcccaatgt
    13981 ggaggtggac cggctgaacg cgtttaccga cctgccggcg tttacggccg agcatgtgag
    14041 cgccaaggtg gcggtcatcg cccgggaggc ggtgccgcag accgcatcgg gcggctgggc
    14101 cgaggtactc gaggtggccg cccgggtgca ggccgccgat acgctgctgt tcgccgtgcc
    14161 gatgtggaat ggcggcatcc catggtcgct caagctgttt gtcgacatcg tcacgcagcc
    14221 cggcgtggcg ttcagcttcg atcccgcgac gggttacacg ggcttgctgg gcggccgtcg
    14281 cgcggtggag gcgtacacca gccgggtcta tgcaccacat gtgccgccag cgttcggagt
    14341 cgatcaccaa tccagctatt tcaactggtg gctgaagtac tgcggcatcg aagacgtgca
    14401 cgaattgcgc ttacagccaa catttcccgg cgagaagttc actgcggaac gcgaccgggt
    14461 gacagcgcag gcgcacgccc tggcacgcac gctcgccggt caggaggttg cgcgctgatc
    14521 ctcggtgacc gattcgatca gcattcgcaa gtgtttgttg acctcgtccg ggcgctcgac
    14581 gatcgcgcaa tgcccgccgg acaactcgac gaacgacgcc aagttcggca ccatactggc
    14641 gatgcggcgt gacgaaacca tcggcagcag acggtctttc tcgctaccga tgaccagcgt
    14701 cggcaccgtc agattggtca gcccgatgtg ttcgggaccg agcgcgtcga ccagcgtgcg
    14761 ggcccagccg ccgcggcccg cgggcggtgt cgaggtgaac aactcgtaga tgaactcgac
    14821 gacagccgga tcggcgtccc gacctaccgc gatcgtggag acgaactgcc ggctcggccg
    14881 atccacggcg cgcaaaagcg gtgcggcacc gaaggtcttg agcagagtcc ccgccgcgcg
    14941 gacccgcgca tcggcgaaag gcggaggcac aggcaggaat tgcacatgcc gaagcagatc
    15001 acccgtggtg gtgttgatca gagcgacggc gtccgcgcgc tgaggcaccc gatccggata
    15061 ccgctccgcc cacgacgtga tcgcgatgcc gcccatggag tgtcccgcga tgaccgcgcg
    15121 ctcacccggg gccagcgtcg cctcgagaac ggagtcgaga tcggctgcga ggtggtcgag
    15181 gccgtagaag tggcgacgca ccggaacggc gctgcgcccg tggccgcggt ggtcgaatgc
    15241 aatgacgcgg tagtcggtgg acagctccgc gatctggtaa gcccacaccc ggatggcgca
    15301 cgtgatgccg tgggcgagca cgatggggta gccatcttca ggcccgaaga cttcggcgtg
    15361 cagccggatg ccgtccttgg accggacgcg aacggtgcgg cccttcggca atgcctcggc
    15421 cgcggcgaat tttctggccg gtgagccggc ctcctgacca cgtcccatgg cgagctccca
    15481 tcgagttcgg caacgttgcc gtagtgccca gcctaccggt cggttgggcg caactagcgc
    15541 aggagtttaa tgatgcgatg cgcgcgatac agatagcccg gctcgacggc ccgcaggccg
    15601 cggaagtcgt cgatatcgaa gagccaagcg ccggtgatgg cgaggttctc atcgacgtac
    15661 acgccgcggg cgtggcgttc cccgatgccc tgcaatcgcg agggctctat cagtacaaac
    15721 cggagatgcc gtacacccca ggggccgagg tcgccggagt ggtgcgcagc gcaccggatg
    15781 gggcgcacgt gaaggcaggc gatcgcgtcg cggggctgac aatgctgtgc ggggcgatgg
    15841 cggaagtcgt tgcgttgcaa ccggatcgag tgttcaagct gccggacacg gtgtcattcg
    15901 aggcgggcgc cgggctgctg ttcaacgatc tgacggtgca tcacgcactg cgcacccgcg
    15961 gccggctggc cgagggtgag agcgtcttgg tgcatggcgc ggcaggcggg gtcggcacgt
    16021 cggcgttacg gctcgcgccc gcctggggcg cgggccgcac gattgcggtg gtgagcaccg
    16081 atgacaagat cgcggccgcc aaggcggcag gagcatcgga tgtggtgctg gccgacggat
    16141 tcaaggacgc cgtcaaggag ttgaccggtg ggcgtggcgt ggacatcgtg gtcgacccgg
    16201 tcggtggcga ccggttcacc gactcgttgc gctccttggc cccgggtggc cggctgctgg
    16261 tcatcggctt caccggcggc gacatcccaa ccgtcaaggt caaccggctg ctgctgaaca
    16321 acgtcgatgc cgtgggcgtc ggctggggag cctggtcgct gacccatccc ggctatctgg
    16381 gcgagcagtg ggcggagctg gagcccctgc tggcgtcggg caaggtttcg gcacccgaac
    16441 cgatcgtcta tccgctcgag cgcgcggcgg aggcgatcgc atcgctggag gatcggactg
    16501 cgaagggcaa ggtcgtcgtc aagctgcgat gagcgcgtcg cgctccgcga gcgatttcag
    16561 gacgagcaat tgcttgcgca tcatcagcag gttcaccaac gccagcgtct ggccaccaac
    16621 cacggcccgt cgaagagcac gcgcacgtgc agccgggtgc cgtcgccgtc cgggcgcacc
    16681 gcgtaggtgt tgctcaccga tccgaacctt gacatcacac tgaccttgtg tggccgcagg
    16741 gtgatgtgct cgtcatgggc gaagctcgcg agctcgaaaa cgtatgagcg cccgaatatt
    16801 cggacgaacg gctgaccgac ggccagatcg gtgagcaccg cacaccgaat gcgcgggctg
    16861 cgacgtccag tgttgtcgag caggtcatag ctgtacggcg caatgcgcag ctggcacagc
    16921 cacgcgaaca ctatcgcggt cgatgcggcg atagaaatcg cacggtcgag gcgaacagcg
    16981 tcgggcatga gctcatcgca tggcaggcgc gccgcgcgtt cggcaggcgt gacgtaccag
    17041 gttgttccga gcatgctgct tatattatcg aatgtctata tagataacaa tagatataac
    17101 ggcaccgacg tttcgagatc gagtcgacgg gtacgccgcg cccatggtta agcgtgtgat
    17161 gattgccggc gccttggtca tggcacccac tctcggagta agtggatgcg cgacgggaaa
    17221 ccagtcggcc aacgaaacaa ccacgtcgag ttcgccccag accagcgcca gcctggcgcc
    17281 gggcacggaa cgactgagca ctcagctcaa gagcgctgac ggaaccccgg tggccaatgc
    17341 gaccctcgac ttcgcgaacg gatatgcgac cgtcaccgtc gagaccgtcg ccgcggggat
    17401 tctgacgccc ggcttccacg gcctgcacat ccattcggtc ggcacgtgcg aggccaactc
    17461 ggtagcaccc actggcggcg cgtcaggaaa cttcaattcc gcgggaggcc acttccaggc
    17521 gccgggccac accggtcatc cggcgagtgg ggacctgacc tcgctggagg tgcgctcgga
    17581 tggctcggca ctgctgacga ccacgaccga cgcgttcacc gctgcggacc tgctagccgg
    17641 cgggaagacg gcgttgatca ttcacgaaaa gcccgacaac ttcggcaaca tcccggcgga
    17701 tcgctatcag cagaagggcg gcgcggcagg cccggacgaa gcaaccctgt cgacgggaga
    17761 ctctggtggg cgggtcgcct gcggagtgat cgaatccgcc tccgccacaa cgacatcgtc
    17821 gacaacgacg acaacgacgc cgagcaccac cgtcaccgaa gccccggtca ccacgaccag
    17881 cccggcgacg acgcctccgc ccaccaccgg cacgagcacc gtcacagtca cggttccgac
    17941 caccacgacg gttcccacga gtccaccgag caacccgatg cagccgggca ccaccacgcc
    18001 accgggtggc tgacgcctac gcgctcagcc cttgcgctag tttctcggcc accgtggcga
    18061 gcagttcggc cgcgtgactg atcgaccgct cgcggtccgg ttccacgtcg ctgagcgcgt
    18121 agaggcctgc gaatcctgcg gcgcgggcct catccgcagt caatgcagac actccggcga
    18181 ccgcgaaggt cggcaccctg tagctgccgg cacgccgaca cactcctacc gcggccttgc
    18241 cgcgcagcga ctgtcgatcg agcgagccct cgcccgtcac aacggcgcac gcatcttgca
    18301 ggtgcttgtc cagttcgatg cggtcgagga tcacgtcgat gccgggctgc atccgcgcgc
    18361 ccaggacagc cagtgcggca aaaccgacgc cacccgcagc gcctgcccct gcggcagttc
    18421 gatggtcggt tcgtgtttcg tgctcgacga cgtcggccca gcggcgaagg gcccgatcga
    18481 gttcgatcac ttcgtccggg tcggcgccct tctgcggtgc gtagacgctc gccgcgccaa
    18541 gtggaccgta cagcgggttg tccacgtcag aggcgagagt gaatgaggca caagcgatgt
    18601 cggggtgcag cccagtgagg tcaagctgcg ctgccagtcc gatgttctgg cccgacggtt
    18661 cgatgcgcgc gccgtactga tcgcgcacgg tggcgccgag tgcggtgaga aagcccgcgc
    18721 caccatcggt gcttgcgctg cccccgacgc cgatgatgat gtcgcggcat ccgtgatcga
    18781 gggcctgcgc cacgaccgcc ccgaggccat ggctggtggc agtcatcggg gccgggacag
    18841 ctccgggaag ccgttgcagg ccacagattt cagccatttc gacgacggcc gtcttgccgc
    18901 ttcgggcgta tgcggtatgg acgacgtgac ccgtgggccc cttggcatag accgggatcc
    18961 gctcgaatcc ggccgcaaga accgtgccga gcgttccgtc gccgccgtcg gcgaccgggc
    19021 acagcaccgt ggtcaatgtg gggtcgccgc gtttgaggcc cgtggagatc gcatctgccg
    19081 cctcggcggc cgtcagtgag cccttgaact tgtcgggcgc gatcaccaca cggccaccca
    19141 tcggaccacc atccatctgt cgagattatt ccgcaatacg gaataccgat ttcaacgaaa
    19201 acggtggcat acgctaaagc caccgtcaag gcgacacatc cagttcgttt gcgttaccgg
    19261 tggtccaccc ttgacaaggc ggtgcctcag ggcgttgact attctgcatc acgagagaaa
    19321 tattccgtat tacggaatag gagtagacga tggcgatgct gtacggcgcg ctcgcgatgt
    19381 tcccgctggc gtgggccacc gctggctacg tgctggtgtc gcacactccg ttggcaagaa
    19441 cccagaaagg aaagccgaga tgacggcccc ctatacggtc aactgctcga tcctgctgac
    19501 cgatctaccg ctgctggacc ggccgggcgc ggcgcgcaac gcgggctttg aggcggtcga
    19561 gttctggtgg ccgttcgact ccccaacgcc gccggacgcc gacgtcaacg ccttcatccg
    19621 cgccattcag gatgcgggtg tgcaactgac ggggctcaac ttcgccgccg gtgacatgcc
    19681 cgcgggcgat cgcgggattc tgtccgatcc cgccttgacg caagcgtttc gcgacaacgt
    19741 cgagatcgcc gtcggcatcg ccgaaactct cggaacccgc gccttcaacg cgctctacgg
    19801 caatcgcatc gacgacattt cacccgccac ccaggatcac gtcgccgccg agaaccttgc
    19861 gcacgccgca cgcgcggcgg accgcatcgg cgcgaccgtg ctggtcgaac cagtgagcgg
    19921 ggcacctcgg tatccgcttc tcaccgccgc cgacgccatc cgcgtgatcg accgggtgca
    19981 caaagaacac ggcgcaacga acgtgcggct gctggccgac ttctaccacc tccacgtcaa
    20041 cggcgacgac atcaccgccg cgataaacga ttacggcagc cggatcgggc acgtacagat
    20101 tgccgacgcc cccggtcgtg gtgagccggg taccggcgaa atcccgatcc ggacctacct
    20161 cgagcagctg gaggcccagg gctaccgggg gtacgtcggc ctggaataca aggcgaccaa
    20221 accggacacc ttcgaatggc tgccacgggc cgagcgcggc gcaaccttgc aaccacacgc
    20281 agaaacgagg atctaatgag cacaatcgct ttcatcgggc tgggcatcat gggcagcccg
    20341 atggcacgcc acctgatcaa ggccgggcac acggtcatcg gcttggacac gtcgcaggaa
    20401 cgcatcgccg ccgtggtcga ggcgggaggc aaggccgccg attccatcga gcaggcggtg
    20461 aaaggcgccg acgtcgtggc catcatggtg cccgactccc cccaggtgca gagcgttctg
    20521 ctgggagacg acggggtgtt cgcccacgct caacccggca cgctcgtgat cgacttctcg
    20581 tccatccggc ccgacgtcac ggcggagttc gcgaagatcg gcgccgagcg tggactgcgc
    20641 attctcgacg cgcccgtgtc cggcggtgag cccggcgctg tgaacgccac gttgtcgatc
    20701 atggtcggcg gctcggaggc cgacttcgcc accgccaagc cgattttcga cacggtcggc
    20761 aagacgatcg tgcatgtcgg cgccaacggc gcggggcaga ccgtcaaggc ggccaaccaa
    20821 ctgatcgtcg ctggcaacat cgagctgctg gcagaggcga tcacgttcct gaaggcctat
    20881 gacgtcgaca tcgatgccgc ggtcaaggtg ctcggcggcg gcctggccgg ttccgcggtg
    20941 ctcgaccaga aggccggcaa gatgctggcg cgcaacttcg acccgggctt ccgcatcgcg
    21001 ctgcaccaca aggacatggg catcgtcacc gccgctgcgc gggaagcggg ggtggtgacg
    21061 ccacttggtt cgctggtcgc ccagctgatg gcatcggcga tggccaacgg cgaaggcggc
    21121 cttgaccatt cgggtctgct gttgggcgtc gagcggctgt cgggcaagga aacagctcaa
    21181 tgacacggat gcgaacggtc gacgcggcgg tcagaatcct cgaaatcgaa ggcgctaccc
    21241 aggctttcgg cctgcccgga gcggcgatca acccgttcta ctcggcgatg cgcgcgcacg
    21301 gcggcatcaa gcacgtcctc gcgcgccacg tcgaggccgc cagccatatg gccgagggct
    21361 tcacccgcgc cgcgccggga aacatcggcg tgtgtatcgg cacatccggc ccggcgggca
    21421 ccgacatgat caccgggttg tattccgcct cagcggattc cattccgata ctggcgatca
    21481 caggccaggc accggtggac aggctgcaca aagaggactt ccaggccgtc gacatcgccg
    21541 cgatcgccga gccggtgacc aagatggcaa tgacggtcct cgaacccggt caggtgccag
    21601 gtgcgttcgc tcaggcgttc cacctgatgc gatcgggacg gcccggtccg gtgctgatcg
    21661 atctgcccat cgatgtccaa ctggccgaga tcgatttcga ccccgaggcc tatcagccgc
    21721 tgcccgtgta caaaccggcg gcatcgcgag cgcaagccgt aaaagccatc gacatgctgg
    21781 tgtcggccga acggccgctg atcgtcgcgg gcggtggcat catcaacgcc gacgcgtccg
    21841 atctgctggt cgagctggcg gagatcctca atgtccctgt ggtgccgaca cttatgggtt
    21901 ggggcaccat tccggacgac cacggcctgg cggcaggcat ggtgggactg cagacgtccc
    21961 accgatacgg caacgcgacg atgctcgcat ccgacttcgt tctcggagtc ggtaaccggt
    22021 gggccaaccg ccataccggt gggctggaca cctaccgcag gggacggcgt ttcgttcacg
    22081 tcgacatcga gccgacgcag atcggtcgcg tcttcgcccc cgacttcgga atcgtctcgg
    22141 acgcgaaggc ggcgctcgaa cagttcgtgg cggtcgccaa ggagcggcgt gccgcaggca
    22201 cgctgccgga ttggagcgcg tgggtcgagg aatgccagca gcgcaagcgg acgatgcatc
    22261 gcaagacgca tttcgacgac gttccgatca agccgcaacg cgtctacgag gagatgaatc
    22321 gcgtgttcgg ccgcgacgtc cgctacgtca ccgcgatcgg actatcgcag atcgcgggcg
    22381 gacagttcct tcaggtgtac ggcccgcgga actggatcaa ctgcggtcag gcggggccac
    22441 tcgggtggac ggtgcccgcg gcgctcggcg tcgtcgccgc cgatcccgac gcgaccgtgg
    22501 tgggactgtc gggagactac gacttccagt tcctgatcga ggaactcgcg gtcggtgccc
    22561 agttcaacct gccgtacgtc catgtggtcg tgaataactc gtacctgggg ttgatccggc
    22621 aggcgcagcg gaacttcgac atgaattact ttgtgtcgct gggcttcgac aacatcaatg
    22681 cccaggagac cgatgacagt gacgtcccca agggctacgg cgtcgatcac ctgcgggtcg
    22741 tcgaaggttt cggctgcaag gccattcaag tggtcgagcc gcaacacatc ggtccggcgt
    22801 tgacgcgcgc gcagcagctc gcccgcgagc acaaggtgcc cgtggtggtc gaggtctttc
    22861 tggagaagat cacgaacatc gcgatgggca ccgagatcga caacatcgtc gagttcaacg
    22921 atctcgccga aacgctggag gacgcaccga cggccgaggc gctgctcctc ctcgactaga
    22981 ccgcggcctc tttgtcgaat tcggaggcga tatcgcgggc gacgcgtttg agcagtggcg
    23041 tcactttcgt cgccgacttc agcgtgaggc gcgccgccgg acccgagatc gagatcgcag
    23101 tcaacgtggg ggaatcgggc accggcaccg cgaagcaccg cacgccgatc tcctgctcgc
    23161 cttcgtcgac ggcgtagccg cgggaccggc tcagttcgat gtcgcggatc agtgcttccg
    23221 gagtcgtgta cgagttctcg ttggaggccg gcatgcccgc gcgggtcagc agcgcgtgca
    23281 cattgtcgtt ggtgagctgc atgagcagcg ccttaccgac cccggtgcag tgcaggtgga
    23341 cgcgccgtcc cacttcggtg aacatccgca tcgcatgcgg tgagggcacc tgagcgacgt
    23401 acaccgccat gtcgttgtcc aacacggcca tgttggcgct ctcgcccgtt cgctccacca
    23461 gctcggtcag atgggggcgg gcccatgctc cgatgagttg cgccgcactt tcccccaggc
    23521 gaatcagttt gggccccaac gcatagcggc ggttctcgag ctgccgcaca tagcccatca
    23581 ccaacagcgt gcggaccaga cggtgaatcg tcggctgcgg cagctccgca cgagcggcca
    23641 actcccccaa cgcgatactg ccgcccgagc tcgccatgat ctccagcagc tcgaatgcgc
    23701 gctcgaccga ctgcacaccg tccgccactg aaccctccgc tcgaccacag cttgcgactc
    23761 cgtttggcga caagcttatt ccgtatgacg gaattatccg ctatctggcg ggggtcagca
    23821 acgaccctgt gggcgacatg tctcagcggc gatggggggc taattggatt ctggcgactg
    23881 tatgctgtga gcatcctgcc gtgaccggaa gggcagccca tgactcaacc gttgatccgt
    23941 ccccgaggga ctggactcag tgctgcgacc acccggaccg ggccgccacc agtcgaacgg
    24001 cccgccccgt tgcgcgaggc cgtttacgac gcgctcgtcg agatgatcat caccgcggag
    24061 ttgcagccag gccagcacct agtggaaaac gatctggctg cgcaccttgg ggtcagccgg
    24121 caacccgtcc gggaagcgct gcagcgcatg cagactgagg ggtgggtgga cctgcgtccc
    24181 gcgctcggcg cattcgtgca cgtgcccacc gaggatgaag ccgaccaact gctcgccgcg
    24241 cgcaccctgc tcgaaacgga gtctgcgagg ctggccgccg agcacgcgac gcccgcgcag
    24301 gtcgagcatc tctgggagct gcaccgcgct ggcgaggcgg cgctcgccgc agacgaccag
    24361 gagggcctcg tcgccgccaa cgcctcgtta cacagccaca tcgtcgcgat gtcgggcaat
    24421 aaagtgctgg cggatctcat cgggtcggtc gatcgccggg ttcggtggta ctacttaccg
    24481 attgctcggg cccgcgggaa agatgcgtgg gacgagcacg ccgcactgat aacagcgatc
    24541 gccaacggcg atggcgtccg gaccggcaac ctgatgcgcc ggcacaccga acgcaccagg
    24601 gagacgtatc accaacggcg tcggaaggcg gccgaggaaa ccacctgatc tcctcggccg
    24661 ccccgcgact cgcttagtcg gccaacgtgg gtgctaacga ctcctgtgtg gtggatgcgc
    24721 tcgacttggg cccgggctcc cgacgccccg gctgccggag gaaggtagcg atacccgccg
    24781 acaccagagc actcgccgcg gcaatccagt aggcgccggt gtagccccac gcgtcaatca
    24841 cactggcgcc cacaccgatt ccgacgatcg agcccaccag cttggagctg tagaccaggc
    24901 cgtagttgct ggcgttgttg ttctcaccga agtagtccgg cgtgagcgag gcgaacatcg
    24961 ggtagaacgc gccgccgccg aagccgacca ggaaagcgaa ggccaggaag gcgatctcgc
    25021 tctcggcctt accggcgtag agcagcccga ccagtgacag cgcctcgatc agcaggacaa
    25081 ccagcagggt ctgcttgcga ccgaatcggt ccgacaacca gccagtcacg gtacgcccgg
    25141 tgccgttgat gatggagagc acgcctgcgg aggacgccgc gatcaacggc ccgaacccga
    25201 ggtccttggc gaacggaacc atgtagctga tgccgaaaag cgagacaccg gcgctgattc
    25261 cgagcgacag ccacatcagc ggcaacatcc cggtcttgat tgcctccatc ggcgtgtact
    25321 gccggacggc cggtgggttc ttcttcaggc tcgccgcgcc gttcttgctg tccgcccact
    25381 tgaccgggtc gacgtcggcg ggccaccagt tcttcggcgg atcgcggaag agccagccac
    25441 ataccgcgca gacgatcagc atgtacgcgg ccaccaggtc caggacccag acgtaggtgt
    25501 tcggatgcaa cgcgtagctg aagatgaaga tgaatggcac tgcaccatag gcgaatccgc
    25561 cgttgacgaa tccggtcttg ccgccgcgcc gctccggata ccacttgccg accaggttga
    25621 tgcaggtcgc gtacaccaga cctgccccga tgccgccgca tacggcgaag ccgaggtagg
    25681 cgagcactag gttgccttgg gtgatgctgg cgaaaccgag cgccgaaagg accgcgccga
    25741 tcagcatggc ggcccggggc gagaggatgc ccttttcgcg caacttgcct gccgggaaag
    25801 ccacgccggc ctggaagaac gcccagatcg acagcagcca gaacgtttcg cccatgctcc
    25861 agtggtgcgc atcccggatg gatttggcgg cggcgccgta tccgtactcg tacacgctga
    25921 cggccatcat ggccatccac ggcagccaga tcatccacgc acgggacctg ccgagcagtt
    25981 cacgatcggt ctccccgact cgataaacgc ggccgttcgc gtcgatgacc tcgcgataac
    26041 ttgcgaaagt tgacgacatg cttgattctc tttctacgaa tggttgaagg ctcgcttcac
    26101 acgggtgcca gccggtgtgt agcaatcacg caccgcggct gcggttttcg tgcggcatgc
    26161 cgccgccccg gtctatcgca tgacggttct gtcctttcgt cgccgagcca cttgctcaga
    26221 tgacgccggc actcctgaac agctcgagct gctcgggcaa atagccgagc tccgtgcaga
    26281 tctcgtcggt gtgctcgccg agcagcgggg agcggacgat gtccactgga gagtccgaca
    26341 gccgcagcgg cgagccgacg gttttgaacg ggccacgttc ggggtggtcg acggtgacga
    26401 tcgccccgag ctcggccagc gtttcgtcct cgatcagctc cttggtggac agcaccggac
    26461 cgcacgggat gttgagcgcg ttcagcgcct ccaacgcctc ccatttggtg tgccgctcgg
    26521 accagtcctc gatcagcgca aagaccttgt ccagcttcgg aaggcgatct tccggccgcg
    26581 cccagtcggg gtgttcggcg agatcgagcc tgccgatcag ctccgccagc gacgcccagc
    26641 cctggggctg cacgatcacg tagatgtagt cgttggggcc gccacccttg gtcttgactg
    26701 cccatcctgg ttggccgccg ccggaggcat tgcctgatcg ggggacctcg tcaccgaaac
    26761 gctcgttcgg atactcgtgg agcggtccac tggcgagtcg ttgttgatcg cggagcttga
    26821 cgcggcatag gttgaggacc gcttcttgca tggcaacctc aacacgctgt ccgcggccag
    26881 tattcgtgcg ctggtacagc gcggccaaga tgcctgacac aaggtggatt ccggtgcccg
    26941 agtcaccgat ctgggcaccg gtcgcgacgg gcggtccgtc ctcgaaacct gtggtagcca
    27001 ttgagccacc catcgcctgc gcgatgactt cgtatgcctt gaagtcgaaa tacctgcccg
    27061 gcccgaaccc cttgatcgag gcgtagatga ggcgcgggtt gatctcgcgc agtcgatccc
    27121 aggtgaaccc gaaccgatcg acgacgcccg ggccgaagtt ctcgacaagg atgtccaccc
    27181 gcgacaccag atcggtgaag atccgcttgc cgtcgtcgct cttcatattg agggtgatgc
    27241 tgcgcttatt gcagttgagc atcgtgaagt acaggctgtc ggcaccggga acgtcgcgaa
    27301 gctggccgcg ggtgacgtcg ccggttttcg gcgcctcaag cttgatgacg tccgcgccga
    27361 gccaggcgag caattgcgtg gccgacggtc ccgactgcac atgcgtcatg tcgagcactc
    27421 gaacgcccgc gagtgcctta cccgtcggcg gatcggattc cacgaccgtg gggatctgaa
    27481 ccatcactaa acttcctttg actcgagtcg gtttgcttcc atgcgttgtg aagctcgtcg
    27541 caggattgca taccgtatga ggtagacaac agtgtcgggt acgcaacatc gcttgtccag
    27601 cgatcgggca gtactcacag acttctctgc caggcacgaa atcgccggct cccagatcgt
    27661 cgatgcgtgc ccccttgcgc gcaacgggcg acggcatgcg tttcatcgat acttgttggc
    27721 ggacaacgga ttacaccaca ccgcaccgtg ccaactatca cgccggcccc tgcgaagcgg
    27781 cggcatcgta ggcgggtggg acgaacaggt gaagccgcgc caggaaatcg acaggaaact
    27841 cggggcttgc atcaagattg tatgaggtat accgtacgcc gtgtgatgtc cgttactttc
    27901 tgacgaggcg agtgacagcc ttcacaacac actgtcgata gcagctgtgc cgggtccggc
    27961 aagcggtcac tgatcaacgt gtggttcggc tgcaacgcgc acgcgtcgca gccatgaacc
    28021 agacggtgca gaaataaggg gactgtcttg ggaaaggcac tcgagggcgt gcgcgtcctg
    28081 gacatgacac acgtccagtc cggcccctct tgcacacaga ttcttgcctg gctgggcgcc
    28141 gacgtcgtga aactggaggc cccctcaggc gacgtcacac gtaaacagct gcgggacatc
    28201 cccggagccg acagcctcta cttcacgatg ctcaactgca acaagcggag catcacgctg
    28261 aacatgaagt cccccgaggg caagcgcatc ttcaccgagc tgctcccccg gttcgacatc
    28321 ctcgccgaga acttcggccc cggcgcgatc gagcgcatgg gcttcgggtg ggacgagctg
    28381 cagcgcatca acccgcggct gatctttgcc tccatcaagg gttttggcga cggaccctac
    28441 acccactaca aggcctacga agtcatcgcg caggcgatgg gcggctcgat gagcacaacc
    28501 gggttcgaag acgggccgcc gttggccacc ggtgcgcaga tcggcgactc gggcaccggc
    28561 attcacaccg tggccggcat cctggccgcg ctctaccagc gcgagcacac cggccgcgga
    28621 caacgcgtga ccgtcgccat gcaacacgcg gtgctgaacc tgtgccgggt caagctgcgc
    28681 gaccaccaga ggctggagca cggaccgctc ggcgaatacc ccaacgagga cttcgacgac
    28741 tgcgtcccgc ggtccggtaa cgcctccggc ggcggccagc ccggctgggc cgtgaaatgc
    28801 gcaccgggcg gacccaacga ctacatctat gtcgtcgtcc agccggtggg ctgggagccc
    28861 atcgcgaacc tgatcggaag gcccgagctg gtgaccgatc ccgaatggag tacaccccag
    28921 gcacgcctga acaagttgga caagatgttc cagctcatcg aggaatggac gatggggctt
    28981 ggtaagtggc aggcgctcga gctcctgacc gaacacaaca tcccctgcgg gccgattctc
    29041 tccaccaggg agatcgtcga cgacacctca ctggcggcca acgagatggt ggtccgggtc
    29101 gatcaccccg agcgtggcga gttcaccacc gtcggctgcc cgatcaagct ctccgactcc
    29161 ccggtcgaca tcgatcgctc gcctttgctg ggcgagcaca acgcgcacat ctacggcgac
    29221 gagctcggcc ttggcgacca gctcgtcgcg ctgaaggcga agggagtcat ttaacgattc
    29281 cgctacccaa aaaggcagac agtcggcagt atacaaagac gaaaaggaga tcgacacgca
    29341 tgaacgcgtc agctcagccg gcataccgcg aggtagtcga cggtaacggc agggtgtacc
    29401 gggtcgggga gaccgaccgc gacatcctgg gacggtcacg ggcatggatg gtatggctcc
    29461 cgtggatcgc gatgatggcc atcagttcat ccgaatatgc gttcacctct gccgaggaca
    29521 cgctgtcgga ggcccatggc tggcacggcg cccatatctt ctggttgctc ggcgtatggg
    29581 tgctgttcca ggcggcggtg gccttccccg ccggccggct gcgggaaagt ggcaggctgt
    29641 cggcacggtc ggcgatgctg ctcggcgccg ccggggcgct tctcggctat ctgtcgctgg
    29701 cgtacgcccc gcacgtcctg cttgcatata tcggcttcgg gttcttcggt ggcggcggag
    29761 ccggcttcgt gtacgccacc tgcatcaaca tggttggcaa gtggtatccg gaacgcaagg
    29821 gcggcaagac gggcttcgtc aacggcggct tcgcgtacgg gtcggtgccc ttcatcttct
    29881 tgttcaccaa ttacatggac ctggagaact acgagggcat cctgttggcg gtcggcatct
    29941 tcctcgccgg cttggtcgcg gcgaccggct tcttcttcgt cgatccgccg aagaactggt
    30001 ggccgccgca cgtcgacccg ctgaaggtca acgacgatcc gcgaatccgc cgcgcgcttc
    30061 gcaagaaccc gccggccgtc aagcagtaca cccccatgga ggcggtcaag accggcatcc
    30121 tgccgctgat gtggttcgtc ctgctgtgca ccgcgggcat caacatcttc ggcatcgcga
    30181 tgcaggtgcc gttcggcaag gagatgggtt ttgccggcgg catcgtcgcg ctcgcgatga
    30241 gcctcaaggc catcatcaat ggcaccggcc gcggcgtgat cggctggatc tccgaccgag
    30301 tcggccgcag ggacacgctc atcatcgtct gcgtggtgct cggtctggcc caatacgccg
    30361 tgtacttctc cggttccatc ggcagcatgc cgctgttcct gttggcgtcg atgatctccg
    30421 gattcggcgg cggggcgata ttcccgctgt tcgcggcgat gaccgccgac tacttcggcg
    30481 agaacaacaa cgccaccaac tacggcctgg tctacagctc gaagttgatt tccggtctgg
    30541 tcggtgccgg cctcggcgcg gtcgtggtcg acacctgggg ttacggggcg gcgttcggca
    30601 tcgccggttc ggtcggcatc gcctgcgcct tcatctcggt tctgctgcgt cagccgaagc
    30661 tgaaggagtc gacaccgaca cggcccaccg agccgacgga ggccggggcg agggtattgg
    30721 tagtcgacta gtcggctccg gcgcacagca ccccgggtga tgagacgtcc tgttcggacg
    30781 tcccgcaccc ggggtgttct gttggcggcg atcgggtacg ccatcgacaa gttcgcagca
    30841 aagccagttt attgcatacg aaagtctgta ccctgttgac cgcaggtagc tacttggtcg
    30901 gaggaatgcg caatgtcact tcccaccccg agtccccgta ggcgcggccc cacgctggcc
    30961 gacgccgccg cgcgtcgggt gcaacgcccg gcgcccctgc ggcaggccgt ctacgacgcg
    31021 atcgtcgatc tcatcgtcca gggcacgctg cagcgaggcc agcatctggt cgaaaatgag
    31081 ttggccgagt acctcggcgt cagtcggcag ccggtgcgtg aagccctgca acggttgcag
    31141 accgacgggt gggttgacct gcggcctgca cagggcgcgt tcgtgcatac cccgtccgag
    31201 gacgaggtgg atcaactcct cggcgtacgc accgtcctcg agacgtactc cgcgcgactc
    31261 gcggccgaga acgccacacc cgaggacgtc aaccggctct gggagttaca gcaggacggg
    31321 ctggacgccc tggccgccaa cgactcggaa gcgttggtga aggccaacgt aaacctgcat
    31381 gccgagatca ccgccgttgc gggaaactcc gtgctggcca agcacattga acttgtggaa
    31441 cgccggtggc actggtacta cctgccgatc gcgcagcccc gtggccaagg cgcctgggcc
    31501 gagcacgccg aactgctcaa ggctatcgcc gccggcgatg ccgatctggc gagcagcatc
    31561 atgcaccgcc acacggaaca tacgcggcag gtctaccacg accagcggca gaccaagtct
    31621 gatgccgagt aacgagttga gtcagcacgg aagcggctcg ccggacggga tccgctctac
    31681 tccggaatag acgttcatcg atgttccgcg cagaaagccg atcaacgtca accccgtctc
    31741 cctggcgaga tcaaccgcga gcgacgacgg cgcggacacc gctgcgagcg cggggattcc
    31801 ggccatcacc gccttctgca cgagttcgaa cgaagcgcgc ccactgacca tcagcgtcat
    31861 gccgctcagc ggcagacgtc cctggtcgag tgcccatccg atgaccttgt ccaccgcgtt
    31921 atgccggccg acgtcttccc tcacgcacca caattcccct gccgcgtcga agagtgccgc
    31981 cgcgtgcagc ccgccggtgc gatcgaagac ccgctgggca gccctgagtt tgtccggcag
    32041 ggcggcgatc gtttcggtgt tgagtcgcaa gggatcccgg gcgacgcgcc atctgctgat
    32101 cgtgcgcacc gcctcgagac tggccttgcc gcacaagccg cacgacgacg tcgtgtagaa
    32161 gttgcgctcg accgacacgt caggcagtgg cacgccatcg gccaacagca cgtcgagcac
    32221 gttgtaggtg ttgacgccat ctgcggtggc gcccgcgcag taccggatcc cggcaatgtc
    32281 cgagtcggcc gccacaacgc cctcgctgac gagaaagccg cgggcgaggt cgaagtcgtt
    32341 gcccggggtt cgcatcgtca ccgtgagcgg gcggccggcc actcggatct ccagcggttc
    32401 ctccgcggcg agagagtccg gacgggcagt ggccaccccg tcggccatcc gcacgactcg
    32461 gtatctactg gttacgcgtc ccacgccggc tcccgatggt tatttctccg atggacaagt
    32521 gatccaggtc acgttaaact caccgcatac tgtatgcaat aggagtctac atgtccccca
    32581 ccagcgcacc agtcgcagtc gtcgaagtcc gggaccttca cggacgctcc taccgggtcg
    32641 gtgagcgacc gcacgacatc atcggccact cccggaagtg gatgcttggg ctctcgtggg
    32701 cggcgatggc cggcatcggc gtgctgcagt acggctacgg cgtcgccgtc tccgccctgc
    32761 acgcgaccga cggtgcgagc gtgacgggcg ccctctgggt gctcgccctg tgggtggtgt
    32821 tccaggccgg cgcggctgca cccactgccg cattgagcca ccggtttgca ctggctccat
    32881 cgcgcgccgt gctcgtcggg gcgctcttgt gcgccgcagg cccgttgacc ctcgcctaca
    32941 gcgacagcct cgcgctcgcg gtgctcggat actccgtgct gtgcggcacc ggcgccggaa
    33001 tcgtctatgc cacatgcaca tccaccgttg cgaagtggta tccggaggag cgcgccgtcc
    33061 gcgtcggatt cgtcaccggg gccttcggct atggcgccgt gccgttcatc gcgctgttcg
    33121 tcggcgtcct cacacccgga aaccagacgg aggtcttcgc aggcgtgggc gtgtgcgtcc
    33181 tcctcgtcgt cgccgtgtgc ggcgcgctct tgcgggatcc gcccgcgggc tggtggccac
    33241 ccgagatcga ccccaaactg tgggcgatcg acaagcgggt gaaccgcagt ctgcgcaaca
    33301 acgtcccggc agtgcggcag tactcacctc gcgaagccgt gcggacaccc gcgttcgtgg
    33361 tcatgtacct catcctggtg ttcgccgccg cggcctcgct gctggccatc gtgtacgtcc
    33421 cgttcgtcgc gatgtcccat ggcttttcgc tggccatcgg agcgtcggcg gttggtctgc
    33481 tcgctgtcgt caacggcgcg gggcggagtg tcgccggtcg aatctccgac cgcttcgggc
    33541 gccgccagac gctcagcgcc gcgcttctcc tggagggttg cgcgcagttg gggctcgtct
    33601 attccgcgtc ggtcgggcag cccgcagcgt tcgtggtctt cgctgcgctc agcgggatgg
    33661 caggcggcgc gttctatccg ctgttcgcga gcctggttgc cgactacttc ggcgagcgca
    33721 acgccgtgcg caacttcggc gtggtctaca gcgccaagct gttcggcggc atcatcggcg
    33781 tcgggctccc cgcgttggtg gtcgcgtcgg atggactcac gcttctgttc gtcgccgccg
    33841 gtctgctcag cctatgcgcc gccgtcatga cgcggctgct ccatcgcccc ggataccccg
    33901 cattgcacct gccccggtaa tcgcttagcc cccaaggaga ttcgatgtca agcactgccg
    33961 aacgagaaga gctcaacgag ctgagcggcg cccttttgaa gtgcgtgacg tcgttggcgt
    34021 cgaaatgcgg cgacaccccg gccatgcgtc gcctcgtcaa tgacgccgaa cgcatcctca
    34081 acggcgtcga ccggctggac atcgacgtcg aagagctcga actgacgcgc gggcacaccc
    34141 cgtccgtcga gatgatccag atcccagaca cccagtacga catcgatttc tggagggatg
    34201 tggaccacga gggcattggc gggcagaacg gggtttgcgt gcctgtctcg agcggccgga
    34261 aacgcagata ggtaatcctg ccggtgccgc gccgaggagg tcggtcggca ctcttcccgc
    34321 tggctgctga atcgacttga gctaatccag gaattgctga ccagcagtct tgactccagc
    34381 gtccgttctc ccgcagggta tcggcggcag gcggttgtct cacgagagac gacatatgag
    34441 ggtctcgaaa ctccgtctcg gagccaccga cgctgagcac cgacagcggc gttgtcgccg
    34501 cgggccttgt cgcggccgct gatgatgacg tgggcgccgc gggcggcgag ttgttcggcg
    34561 actgcgcggc cgatgccgct ggcgctgccg gtgatcagcg ctgtttggcc gacgaagtcc
    34621 tgagacacgc ttggctcctt actgtttggg ccgctgaggg cgacggtgtt tgggtggggc
    34681 ggtcccgctg gggggtgagt gggggaccgc cccgcagggg tcaggacacg gtgtccaggg
    34741 cggcgaggat gtcgctgacc tcggtgcggg tggtgtagtt ggggtgcacg tcgatccagc
    34801 ggatcacccc ggtcgggtcg acgatgacga cagtgggcat gggtatgctg taggtgccgt
    34861 cggcgttgac ctcgggcagg tctaaaccca tcgcgcgctg cgcggcttgg gcatcgtcgg
    34921 ggggtgcggt catcacgccg agccgtttgg cgatcttgtt gcccggatcg gacagcacga
    34981 tgaaggtcag cgcgttcttc tcctgggccg acagtgagcc gtcggacttc tgcgggctga
    35041 tggccaccag cgcaacgccc ttgttggcca gtgtagggac gaggtcgtcc tggtaggcgc
    35101 gcagcgccaa attgcagtag gggcaccacg cgccgcggta gagcacgacc accgcggcgc
    35161 tcccgccgag cgcggccgac agtgtggtcg gcg
//
LOCUS       DASRJA010000488         9084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_47530,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000488 DASRJA010000000
VERSION     DASRJA010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9084
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47530"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFC01_21540"
     CDS             <1..483
                     /locus_tag="VFC01_21540"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZN82245.1"
                     /translation="VATGVNLDPDTVGRLAREVDNIRYIKDTSADMAQAGQLIHRHGD
                     VISTFIGWDSLLLAAITEGAAGVMAGTANVMPAQLVSIHRAVTAGDLNRARREWAQLY
                     PLMDAIMSAPFIPAIKTALNALGFPAGIPRKPLAELDAATAATISKLAAELPQFSASA
                     "
     gene            complement(868..1479)
                     /locus_tag="VFC01_21545"
     CDS             complement(868..1479)
                     /locus_tag="VFC01_21545"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN82246.1"
                     /translation="MRLTTVNAIYSRLAKTYDRELRDDMEYTAYLKVPQLVINALGHK
                     QANILDLGCGTGLSSLLFFEKGYDVTGIDGTSAMIRRARKLPYKKVIQQDLESPWRVE
                     DHSFDAVVMIGVMEYIIYPGVLFRQVRNKLVDGGVFGLTVPYKSKFCADVDLKSYYRK
                     EFVPVIRKAGFTIESSEKTLGFQDAGETVLYWNYLLRKRKTTS"
     gene            complement(2077..2526)
                     /locus_tag="VFC01_21550"
     CDS             complement(2077..2526)
                     /locus_tag="VFC01_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN82247.1"
                     /translation="MSSRDDQHQQNADDGSGQATSAFPFDLDDELETPSGAAEPEMAP
                     EELPTGSALLVVQRGRNAGARFLLNRPLTTAGRLPGSDILLDDITVSRRHAEFRIEDR
                     QHRVVDTGSLNKTYVNRRPVESAILANGDEIQIGRFRLVFLSAAPTS"
     gene            complement(2706..3047)
                     /locus_tag="VFC01_21555"
     CDS             complement(2706..3047)
                     /locus_tag="VFC01_21555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN82248.1"
                     /translation="MSRDEVVEASLAGRAYSALSLDKPLDDKTGGDPVPLVATVGALD
                     SNFALIEDRQCVRPLIEALSPCVRAVLVMRFFESLTQSQIATRIGVSQMHISRLLAQA
                     LAQLRDRVTDG"
     gene            complement(3374..4426)
                     /locus_tag="VFC01_21560"
     CDS             complement(3374..4426)
                     /locus_tag="VFC01_21560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012690417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZN82249.1"
                     /translation="MASAHTDTETCAPDPDDPRKPDSPTDLTGSSRLYVLRKTAREFT
                     QDQCMDLAAALTYYAVMSLFPALLVVVSLLGVFGRGRSTTEAVLGIADDLLPAQVVDT
                     LREPIGQLVQSPTAGLALVVGVAGALWSASGYVGAFGRAMNRIYEINEGRPVWKLRPV
                     QLLLTLAGLVTVAAAAVMLAISGPVATAVGDALGVGEALVTAWTIARWPVILALVIFA
                     VAMLYYVTPNVKQPRFRWISVGAAVAILAWMIASVLFGVYVANFSSYNKTYGTLGGVI
                     VFLLWLWITNLALLFGAELDAELERARQLQAGIPAEEDIRLPLKDTRVVDKNAAAEDK
                     DVERGRALRQSGGRSD"
     gene            complement(4652..5047)
                     /locus_tag="VFC01_21565"
     CDS             complement(4652..5047)
                     /locus_tag="VFC01_21565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN82250.1"
                     /translation="MRKRWSNRVVVLSVRETVDILSAPQLSEAICEALGQAPTGLIVD
                     LTDVDFLASVGMSVLVSAHYVADAMSVRFGIVADGPATSRPIRLLGINRILTLYPTLD
                     DAFREFRGGTSGQGHSRLLDDESTTTQSP"
     gene            5280..5948
                     /locus_tag="VFC01_21570"
     CDS             5280..5948
                     /locus_tag="VFC01_21570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN82251.1"
                     /translation="MAARGNSPITVALVDDYDVVVMGVAKMFDQYRDRVVVAELDSNE
                     AVQDVVDIALYDSFAQPESDHEEIAVLVANPRARRVVVYTWNFHPDLIRSAQRQGAHG
                     YLSKTLPARELVAALEAVHAGERVISDVPARARSAVGLDWPGRGEGLSDRESEILALI
                     TQGKSNAEVATLTYLSPNTVKSYIRTIYRKIGVGSRTQAVLWGVNHGFTPDHHRIEHW
                     RGGP"
     gene            6156..6442
                     /locus_tag="VFC01_21575"
                     /pseudo
     CDS             6156..6442
                     /locus_tag="VFC01_21575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562490.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
     gene            complement(6577..7419)
                     /locus_tag="VFC01_21580"
     CDS             complement(6577..7419)
                     /locus_tag="VFC01_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyltransferase-related protein"
                     /protein_id="HZN82252.1"
                     /translation="MKTAVITTVHGRHDHLRRQIRGLELSVRPADIHVVIALGDPTIR
                     NVVAATGSPAILIECGSANGGLPVAGGRNIGAAAALNLGADLMVFLDVDCIPGTALIG
                     RYHDAAAQPEHAQALLCGPVTYLPPPGPQGYPLGQLPSQVNPHPARPAPRSTDIVANT
                     DYELFWSLSFAANATTWNRIGGFCELYCGYGGEDTDFAQCAAAANVTMRWVGGADSFH
                     QYHHVSNPPTEHLDDILRNAVVFYSRWGWWPMEGWLHEFENRGLITRDGDGTPSRPNP
                     SEPT"
     gene            complement(7416..8447)
                     /locus_tag="VFC01_21585"
     CDS             complement(7416..8447)
                     /locus_tag="VFC01_21585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007734850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN82253.1"
                     /translation="MIGYYVHHDGFGHLARAISICAQMRRPVTLLTSRDVPKPHRFTD
                     VVRLPRDDSAAHVSEPTAHGALHWVPHHDPGLASRMDAIARWVAHARPEAIVVDVSVE
                     VAVLVRLLSVPVIVMALPGKRIDAPHLLVHRLADHIIATWPKQLCVPAWLRSYEDKTS
                     FVGGVSRFDGREVPATGTARGDHESSSPTRVLVLGGAGSFDGAQAALEEVAADQKNTT
                     WTKLGASDDDWTDDPWPQICDADVIVSHAGQSCIADIAAAERPAVIIPQARPFDEQRA
                     TARVLRQFRLAVVTQRWPDQRAWPALLTHARAREPVRWRRWEVRGAAARAADAIEQTA
                     QRYAGRPPK"
     gene            complement(8422..>9084)
                     /locus_tag="VFC01_21590"
     CDS             complement(8422..>9084)
                     /locus_tag="VFC01_21590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014379156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN82254.1"
                     /translation="TTLAAVSRHTAAAWQGVVGEITVVPNGIDTRRWPVGPGGPHLVW
                     FGRITPEKGTHVAIAAARRAGMPLVLAGPISDQSYFADEVAPQLRDEIYYAGHLSQNE
                     LARLVGAAAAALVTPMWDEPYGLVVAEAMACGTPVVAFDRGGIPELVAPHAGRLVPPG
                     DVAAMAAAIPESIKLSRNDIHQHARLRCSSTATLNRYLSLYRQMMQGPRDRNDDRLLR
                     AP"
BASE COUNT         1711 a         2836 c         2809 g         1728 t
ORIGIN      
        1 gttgcgaccg gtgtcaacct cgatcccgac accgtcggcc ggctcgcccg cgaggtcgac
       61 aacattcgct acatcaagga caccagcgca gacatggcac aagcaggcca actaattcat
      121 cgccatggtg acgtcatctc gaccttcatc ggttgggaca gcctcctgct cgccgcgata
      181 accgagggcg cagccggtgt aatggcaggg accgccaatg tcatgcctgc tcaacttgtt
      241 tcgattcacc gcgccgtgac agcaggtgac ctcaaccggg cacgacgcga gtgggcacag
      301 ctctatccgc tgatggacgc catcatgtcg gcgccattca tccccgccat caagactgcg
      361 ctcaatgctc tcgggttccc cgccggcatt ccccgcaagc cactcgcgga actcgatgcc
      421 gccaccgctg cgacgatctc caaattggca gccgaattgc cgcagttctc cgcatctgcg
      481 taatcaatca ccctgcggcc cagagcgttt cgccgatcct gagattatgt cagctgcaca
      541 aagttacctg gtctcaacac agttggaact tatcgcagtt ggccctgctc aatgaatcgg
      601 tgattcgctc ggtctagttg ttgcatttgt ttcgacacgc tcactgaaga tgtcgcaatt
      661 ggaaccagca tggtgcttaa gttgacatcg tgactgctgc ggcagctgcc aatcactggg
      721 ctcgcgcggc ggccgagtcg gcctcggtgt tctatcgacc ggaggcgagc tcttgtgagg
      781 ttgcgttgcc gcgacctcgg cggctgcggt cgctttcgac tgcgccgcgc cgtaccgagg
      841 cgttcggtcc tttcggggtc agcggaacta agaagttgtt ttgcgtttgc gcaaaagata
      901 gttccagtac agtaccgttt cgccagcgtc ctggaacccc aacgtctttt cggacgactc
      961 gatggtgaag cccgccttgc ggataacagg cacgaactct tttctgtagt acgatttaag
     1021 atccacgtcg gcacaaaact tgctcttgta aggcactgtg aggccgaaaa cgccaccgtc
     1081 gacaagtttg ttgcgtacct gtcgaaacag cacaccggga tagataatgt actccatcac
     1141 accaatcatc acgactgcgt caaacgagtg atcttcgacg cgccagggcg attccaaatc
     1201 ctgctgtatg accttcttat acggcagctt ccgcgctcga cggatcattg cgctggtgcc
     1261 gtcgatgcct gtcacgtcgt aacccttctc gaaaaacagc agtgaactga gtcctgtgcc
     1321 gcagccgaga tcgagaatgt tggcttgttt gtgcccgagc gcattgatta ccaattgcgg
     1381 caccttcaaa tacgccgtgt attccatatc atctcgcaat tcacggtcat acgttttggc
     1441 aagacgcgaa tatattgcgt tcactgttgt cagcctcatc gatggggatg atatcccggc
     1501 gtaaagcctg ccgctcgaca ccacgccatg gtcggcgcag cagcatatcc agctgttaac
     1561 tcccgaagca tgttgcaggg cagttccttc ggaatcgact gatctaggcg cgcgcccgaa
     1621 cgtcccggta ggaacgaatc cgccgggccg ttcgtgtcga cattcgcagt ggcatcgacc
     1681 agggcggctt tgactgccgt attcgtgcaa tctgacccaa ggcatccagg gagggacgta
     1741 ggagcaaagc gagcaggccg gaactcgggc tgctgagcgg agcgcgaccg cggccgcaaa
     1801 tgccgagatg aatgatggac agcagaaacg tagcgcggcc gcaactaagc gtgcgcaggc
     1861 ataccgaatt gaagcgttcg tcgaggacga aaagcctacc gccaacatga tccgtcgaac
     1921 tcatcgtgaa gcttacgcgt ggcattggaa ccgcttagta ctcaacccga cggcctgaac
     1981 gaaacctcac cgccgtcgac gattagtcgc acttgtcgtg ccgctcgacc gcgaagccga
     2041 tatttttatg ctgggcggcg ccacggtgac atagggtcaa ctggtcgggg cagcgctcaa
     2101 gaacaccaaa cggaatctgc cgatttgtat ctcgtcaccg ttggccagga tcgctgattc
     2161 gacaggtcga cggttgacat aggttttgtt gaggctgccg gtgtccacga cccggtgttg
     2221 tctgtcttcg attcgaaact cggcgtgtcg tcggctgacg gttatgtcgt cgaggaggat
     2281 gtcgctgccc ggaagtcgtc cggcggttgt gaggggtcga ttcaacaaga agcgggctcc
     2341 agcgttgcgt ccgcgttgga cgacaagcag tgccgagccg gtgggtagct cctcaggcgc
     2401 catctcgggc tcggccgcac cggacggagt ttcgagctca tcgtccaggt cgaagggaaa
     2461 agccgaagtt gcttgcccgc tgccgtcatc ggcgttctgt tggtgttggt cgtcacgaga
     2521 gctcacgcgc gtccgacctc ctactcgtcg tgttaccgcg cggcacccag atggtcgcat
     2581 tgaatgacta ccccggcgcg gacgttgcca accagcagaa agcccgtttg gggcagggac
     2641 gtgtacttca cgagagcggt acctgcgaag ggtgtggtcg acttgtgctc acacctggag
     2701 gttcattatc cgtcggtgac cctatcgcgc agctgcgcga gcgcctgggc gagcagacgc
     2761 gagatatgca tctgtgacac cccgatccgg gtggcgatct ggctctgggt cagcgactcg
     2821 aaaaagcgca tcacaagtac tgcacgaacg cagggcgaca gtgcctcgat aagtggccga
     2881 acacactggc gatcctcgat cagagcgaaa ttgctgtcga gagcaccaac cgttgccacg
     2941 agcggaacgg gatcgccacc ggtcttgtcg tctagtggct tgtccaacga caacgccgaa
     3001 taggcgcgcc cggcgagact ggcttcaacc acctcgtcgc gggacaatcc gagatgactg
     3061 gccagtcccg ctcacggtcg gtgcgcgtcc tcgtagctga tgccctgcga gtcgaagacg
     3121 acggtcgcct ggtacatatc gatctgcacc tggctcatag cgggcgggtc agtgagccgc
     3181 agcgccgtat tctcagcgac ctggcgtcag gactgcgcgc tcgcaatgtc gcggtcaaca
     3241 ccatcacaag cgatgacgat cgcccgccgg agccgcagat gatctcggcg ctcaaggcat
     3301 acgtaatgct ttggtttcga agcaagccgg ccaaatcacc gatcacactt tcatgctggg
     3361 cgcggatttg gcgtcagtcg ctacgacccc ctgattgcct cagcgcacgc ccgcgttcga
     3421 catccttatc ctccgcggcg gcgttcttgt cgacgacccg ggtgtccttg agaggtaacc
     3481 gaatatcctc ctcggcgggt atcccggctt gcagctggcg agcacgctct agctcggcgt
     3541 ctaattcggc gccaaagagc aacgccaagt tggtgatcca cagccacagc aagaacacga
     3601 tgaccccgcc gagcgtgccg taagtcttgt tgtaactact gaaattggcc acatatacgc
     3661 caaacagcac cgacgcgatc atccacgcca ggatcgcgac cgcggcgccg acgctgatcc
     3721 agcggaacct cggctgcttg acgttcggcg tcacgtaata caacatcgcg acagcgaaga
     3781 tgaccagcgc gagaatcacc ggccaccgag cgatcgtcca cgcagtgacc aaagcttcgc
     3841 cgaccccgag ggcgtcgccg accgccgtgg ccaccggacc gcttatcgcg agcatcacgg
     3901 cggcggccgc gaccgtcacc aggccggcca gggtcagcaa cagttggacc ggacgcagct
     3961 tccacaccgg ccggccctcg ttgatctcat agattcggtt catcgcccgc ccgaacgcgc
     4021 cgacgtatcc cgatgccgac cacaatgccc ctgccacgcc gaccaccaga gccaaacccg
     4081 ccgttggcga ttggacaagt tggccgatcg gctcgcgcag cgtgtcgacc acctgcgcgg
     4141 gcaacagatc gtccgcgatg ccgagcaccg cctcggtggt gctgcggccc cgcccgaaga
     4201 ccccaagcaa agacaccacc acgagaagcg cgggaaacag cgacatcacc gcgtagtagg
     4261 tgagcgccgc ggccaggtcc atgcactggt cctgagtgaa ctcgcgggcc gtcttgcgaa
     4321 gcacatacaa ccgtgacgat ccggtgagat cggtgggcga atcaggttta cgcggatcgt
     4381 ccgggtcggg cgcacaagtt tcggtgtcgg tgtgagcact agccataccg gtcgataccc
     4441 gatgcaccgc cggcccaacc gcaacggccg cgacctagga aggtggcgtt ctggattgct
     4501 gagcgatgca catcagatgc gcctcacggt tcgagccgat gcggcgcccg ctgccaagga
     4561 gacttctcga tggattcact gaccaccgtc attcgcaact ggtggagata cgctccgact
     4621 tcgacttgag tggccggcgg attggaacgc actaaggtga ctgggtagtt gttgattcgt
     4681 catccagaag tctgctatgc ccttgaccgc tcgtgcctcc gcgaaactcg cggaacgcgt
     4741 cgtcgagtgt gggatagagc gtcaggatgc ggttgattcc gagcagccgg atcggtctgc
     4801 tggtggcggg cccgtcagcg acgatcccga atcgcactga catcgcatca gccacgtagt
     4861 gagctgacac cagaacgctc attccgaccg aggcgaggaa gtccacgtcc gtgaggtcga
     4921 caatgagtcc ggttggcgcc tggccgagcg cctcacagat cgcctcggag agttgtggtg
     4981 cgctaagtat gtcgaccgtt tcacgaaccg acagcaccac gacccggttg ctccaccttt
     5041 tccgcacttc gaaaccgtgt tggtgtgcgg atgcggcgga atgggttgat tcggtgacgt
     5101 gatcgaccat ggtggaaccc cttttcgatc attccaagga gcgatcatgg ctgtaggcgt
     5161 ttccgagtcc caccgccatg tgcgtggaag tcagtccggt cgagcggttg ttgagctgat
     5221 cccagtcaac acccctaggg aggatgtgat tacaccccta gggaggtact ttgaggtgca
     5281 tggccgcccg cgggaacagt ccgatcaccg tggcactggt ggacgactat gacgtcgtgg
     5341 tgatgggcgt ggccaaaatg ttcgaccagt accgcgaccg cgtggttgtc gccgaacttg
     5401 actccaatga ggctgtccag gacgtcgtcg acatcgcttt gtatgactcg tttgcccaac
     5461 ccgaatcgga tcacgaagag atcgccgttc tggttgccaa tccgcgcgca cgccgagtcg
     5521 tggtgtacac ctggaacttt cacccggacc tcatccgcag tgcccagcga cagggagcgc
     5581 acggttacct atcaaaaacg ttgcctgcgc gagagctggt agccgcattg gaggcagtac
     5641 atgccggaga gcgggtcatc agtgatgttc cggcgcgggc gcgcagcgcc gtagggctcg
     5701 actggcccgg ccgaggcgaa ggcctcagcg atcgagagtc ggagatcctg gctctcatta
     5761 cccagggcaa gagcaatgcc gaggtcgcga cgctgacata tctcagcccc aacactgtga
     5821 agtcatacat ccgcacgatc taccgcaaga tcggagtcgg cagccggacc caggctgtgc
     5881 tgtggggtgt gaaccacggc ttcactcccg accatcaccg catcgaacat tggcgaggtg
     5941 gtccctgagc cataccctct tgctcctaca cggcgaggcg accccgtcga ctatccgcag
     6001 acgatcctcg aacttgctgt cgcgctggcg ttctcgaccg cggtgcctac acgctccact
     6061 tttatggccg atcaattcgg aaggccgacc cactgagacg gtcggtgcag cggtaatggc
     6121 gctcagggca gccggcgcga tgagatcagc ccctcgggcg ggcctacccg ctaggtgcca
     6181 gctatgacgg ttggggcacc aattttgcgg tgttcagcga ggtggcggag tgcgtcgagc
     6241 tgtgtctgtt cgatgacgac ggcaccgaga ctcggatcag gctgcctgag gtgggcagct
     6301 tcgtgtggca cggcttctta ccagtgtcga tcctggtcag cgctacggct gccgggtgtg
     6361 cggcccgcac gatccggccg cggggcagcg ctgcaatccg aacaagttgc tggtcgacgc
     6421 gtatgccaaa gcgatcgagg gcgactctca accagtttgg attgcaatcc attgcgacgc
     6481 ccagaacgga ggaagtttcg gctggccgaa cagccccccg agttccgtgc ccgttgattc
     6541 gcggtgcccc tactgcgccg gggccgctga gagcccttaa gttggctcgc tcgggttggg
     6601 gcgggacggc gtcccgtcgc cgtcgcgcgt aatgaggcca cggttctcga attcgtgcag
     6661 ccagccttcc attggccacc agccccatcg cgaatagaag accactgcgt tacggaggat
     6721 gtcgtcgagg tgctcggtcg gcgggttcga gacgtgatgg tattggtgga aggagtcggc
     6781 gccgcccacc caccgcatcg ttacgttggc ggcggccgcg cactgcgcga aatcggtgtc
     6841 ctcaccgccg tatccgcaat agagctcgca gaatccgccg atgcgattcc aggtggtggc
     6901 gttcgccgcg aacgacagcg accagaacaa ttcgtagtcg gtgttggcga cgatgtcggt
     6961 actccgcggg gcagggcggg ctgggtgcgg gttcacctga ctcggtagtt ggcccaacgg
     7021 gtagccctgt ggccctgggg gcggcaggta ggtgacggga ccgcacagca gtgcctgcgc
     7081 gtgctccggt tgcgcggcgg cgtcgtgata gcggccgatc agcgcggtgc cggggatgca
     7141 atccacgtcc agaaacacca ttagatccgc gccgaggttc aaggccgccg ccgctccgat
     7201 gttgcgaccg cccgccactg ggagcccgcc gtttgcgctt ccgcactcga tgagaattgc
     7261 gggagagccg gtcgctgcca ccacgtttcg aatcgtcgga tcgccgagcg cgattactac
     7321 gtgtatatcg gcaggccgga cgctgagttc caagccacgg atctgtcgac gcaggtggtc
     7381 atgtcggccg tgcaccgtgg tgattaccgc cgtcttcatt tgggcggccg ccctgcgtaa
     7441 cgttgggccg tctgctcgat cgcgtccgct gcgcgagcgg ccgcgccccg tacttcccag
     7501 cgtcgccatc tcactggctc gcgggctcga gcgtgtgtca atagcgcggg ccatgcgcgt
     7561 tggtcgggcc atcgctgggt taccaccgcg agccggaatt ggcgcagcac gcgggcagtg
     7621 gctcgttgct cgtcgaatgg gcgggcctgc gggatgatca ccgcggggcg ttccgctgcg
     7681 gcgatgtcgg cgatacagct ttggcccgca tgactgacaa tgacgtcggc gtcgcagatc
     7741 tgcggccacg ggtcgtcggt ccagtcgtcg tccgacgcgc cgagcttcgt ccaagtcgtg
     7801 tttttctgat ccgccgctac ctcctcgagc gcggcttggg cgccgtcgaa actacctgct
     7861 ccacctaaaa ccaacaccct tgtcggggag gaagattcat ggtcgccgcg agccgtgccc
     7921 gtagcgggga cctcgcggcc gtcaaagcgg ctcacccctc cgacgaaact ggtcttgtcc
     7981 tcgtaggagc gcagccacgc tggcacgcac agctgcttcg gccatgtcgc gataatgtga
     8041 tcggcgagcc ggtgcaccaa cagatgaggc gcgtcaattc gcttgccggg caaggccatc
     8101 acgattaccg ggacgctaag caaacgaacg agaacggcca cctcaacgga cacgtcgacg
     8161 acgatagctt cgggtcgagc atgggccacc caccgcgcga tcgcgtccat cctggacgcg
     8221 aggcccgggt cgtggtgcgg aacccagtgc aaggcgccgt gggcggtcgg ctcactgacg
     8281 tgtgccgcgc tgtcatcgcg gggcagcctt accacgtccg tgaaccgatg tggttttggc
     8341 acatcccggc tggtcagcaa cgtgaccgga cggcgcatct gcgcgcagat gcttatggcc
     8401 cgggccagat gcccgaaacc gtcatggtgc acgtagtagc cgatcatcgt ttctgtccct
     8461 gggtccttgc atcatctggc ggtacaggct caggtaccga ttcagcgtcg ccgtcgagga
     8521 gcaccgcagg cgcgcatgct ggtgaatgtc gttgcgcgag agcttgatcg actccggtat
     8581 tgccgccgcc atcgccgcca catcaccagg cggtaccaga cgccccgcgt gcggggcgac
     8641 tagttccggg ataccgccac ggtcgaacgc gacgaccggg gtgccgcacg ccatcgcttc
     8701 agcgaccacc agtccatacg gctcgtccca catgggagta acgagcgcgg ccgcggccgc
     8761 gccgacgaga cgggccaatt cgttttggct cagatgtccg gcatagtaga tttcgtcgcg
     8821 cagttggggg gccacttcgt cggcgaagta cgactggtcc gagatcggtc cagccagcac
     8881 cagcggcatt ccggcgcgcc gggccgcggc gatcgccacg tgcgtgccct tttcgggcgt
     8941 gatacggccg aaccagacca agtgcggccc gcccggcccg accggccaac ggcgagtgtc
     9001 gattccgttt ggcacgacgg tgatttcgcc tacgacaccc tgccatgctg cagcggtgtg
     9061 cctgctgacc gccgccagcg tcgt
//
LOCUS       DASRJA010000489         2550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2560826,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000489 DASRJA010000000
VERSION     DASRJA010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2560826"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFC01_21595"
     CDS             complement(<1..221)
                     /locus_tag="VFC01_21595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82255.1"
                     /translation="MGRPHYFLPRTSASRHQDWRIGWPLSTPTTTTSISSGEGNGRAG
                     GLLLRTVAPRSEVAGLDLTGVSGAEWYAPS"
     gene            220..975
                     /locus_tag="VFC01_21600"
     CDS             220..975
                     /locus_tag="VFC01_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZN82256.1"
                     /translation="MDWAFLAAAGVLGGLTGSIAGLASVATYPALLLVGLPPLTANVT
                     NTVALVFNGIGSIWGSRPELEGQRKWIKRIAPAAAVGGAVGATLLLSTPAEGFERIVP
                     ILLGLASVAILLPSASRQEARVAHHQRHTVKVRLEAAAIFLICIYGGYFGAAAGVLLL
                     ALLLRAGGATLAHANAGKNVILGVANGVAALIFAFFAPVNWPAVLAVGIGCLVGSRLG
                     PIVVRHAPATPLRIVIGAAGVALAVKLGIDAYA"
     gene            complement(980..1648)
                     /locus_tag="VFC01_21605"
     CDS             complement(980..1648)
                     /locus_tag="VFC01_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HZN82257.1"
                     /translation="MTTTERRTENTDRGPLDRDELAARIARDIPAGSYVNLGIGQPTL
                     IANYLRPEAGVVLHTENGMLGMGGAAVGADADPDLINAGKVAVTETPGASYFHHADSF
                     AMMRGGHLDVCVLGAFQVSEQGDLANWHTGAPDAIPAVGGAMDLAIGAKNVFVMMNLF
                     AKDGAAKLVPACTYPLTGVRCVRRVYTDYATFDIDTDAGVRVRETFGIGLEELSSRMT
                     VELR"
     gene            complement(1645..2313)
                     /locus_tag="VFC01_21610"
     CDS             complement(1645..2313)
                     /locus_tag="VFC01_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HZN82258.1"
                     /translation="MTRTSICDTADEAVAGIPDGATVLVGGFGMAGMPTTLIDALIAQ
                     GATDLTIVSNNAGNGDTGLAALLAAGRVRKVICSFPRQSDSYVFDGLYRAGKVALEVV
                     PQGNLAERMRAAGAGIGAFFCPTGVGTPLAEGKELRTIDGRDYVLEYPIRGDVALIGA
                     HIGDRAGNLLYRKTARNFGPVMATAAALTIAEVSRVVDVGQIDPETVVTPGIFVDRIL
                     EVSR"
     gene            complement(2310..>2550)
                     /locus_tag="VFC01_21615"
     CDS             complement(2310..>2550)
                     /locus_tag="VFC01_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010841273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN82259.1"
                     /translation="PGLDRRSRSLITLTALIARGHDEELAMHVRAARRNGLTNDEIKE
                     VILQTAIYCGVPDANTAFRIASGVLADYDAQEAGK"
BASE COUNT          416 a          856 c          817 g          461 t
ORIGIN      
        1 gatggcgcgt accactccgc gccggacacc ccggtgagat cgagcccggc gacctcagag
       61 cgaggcgcga cggtgcgcag gagcagcccg cccgctcggc cgttgccctc cccggaactg
      121 atagatgtgg ttgttgtcgg cgttgagagg ggccagccga tacgccagtc ctggtgtctc
      181 gacgcgctgg tgcgcggcaa gaagtaatgt gggcggccca tggactgggc ctttctcgcc
      241 gctgcaggtg tgctcggagg gctcactggc agcatcgcgg gcctggcgtc ggtggccacc
      301 taccccgcgc ttctgctcgt gggtctcccg cccctgacgg ccaacgtcac caacaccgtc
      361 gctctggtgt tcaacggcat cggatcgatc tggggctcgc ggcccgaact cgagggccag
      421 cgcaaatgga tcaagcggat cgctcccgcg gcagcggttg gtggtgcggt cggcgcgaca
      481 ctgctgctgt cgactcccgc ggagggcttc gaaaggatcg tgcccatcct actaggcctg
      541 gcctcggtcg cgattctgct gccgagtgca agccggcagg aagcgagagt tgctcaccat
      601 caacgacata ccgtcaaagt caggttggaa gcggccgcca tcttcctgat ctgtatctac
      661 ggcggctatt tcggagcggc ggcaggtgtg cttctgcttg cgctgctgtt gcgcgccggc
      721 ggtgcgacgc tcgctcacgc caatgccggc aagaacgtga tcctcggcgt cgcgaatggc
      781 gttgcggcgc tgatctttgc gttcttcgcg ccagttaact ggccagcggt cctcgccgtc
      841 ggcatcggtt gcctcgttgg ctcgcgactc ggcccgatcg tggttcgcca cgcgcccgcg
      901 acgccgctac gcatcgtgat cggcgccgct ggcgtcgcgc tcgccgtcaa actcggcatc
      961 gacgcgtacg cctagcggcc taacgcaatt cgacggtcat ccgcgatgac agctcttcca
     1021 acccgatgcc gaatgtctcg cgcaccctga cacccgcatc cgtgtcgata tcgaaggttg
     1081 catagtcggt gtacacccgg cgcacacacc gcaccccggt caacgggtag gtgcacgcag
     1141 gcaccagttt ggcggcgccg tccttggcga acagattcat catcacgaag acgtttttcg
     1201 cgccgatcgc cagatccatt gcaccgccca ccgcggggat ggcatccggc gcaccggtgt
     1261 gccagttggc caggtccccc tgctcggaca cctggaaggc gccgagcaca cacacatcga
     1321 ggtgcccgcc gcgcatcatc gcgaacgagt ccgcgtgatg aaagtaggac gcgccagggg
     1381 tttcggtgac cgcgaccttg cctgcgttga tcagatcggg gtcggcgtca gcgccgaccg
     1441 ccgcaccacc catgccgagc atgccgttct ctgtgtgcag cacgacaccg gcttcaggtc
     1501 gcagatagtt ggcgatcaac gtcggctggc cgatgccgag gttgacatac gatcccgccg
     1561 ggatgtcgcg cgcgatcctg gctgccagct catcgcgatc caacggtccg cgatcggtgt
     1621 tttcggtacg acgttcggtc gtggtcatcg gctcacctcg aggattcggt cgacgaaaat
     1681 gcccggcgtg accacggttt caggatcgat ctgaccgacg tcgaccactc tggagacctc
     1741 ggcaatcgtc agcgcggcag ccgtggccat caccggcccg aagttgcggg cggtcttgcg
     1801 atacaacagg tttcccgcgc ggtctccgat gtgggcacct attaacgcga cgtcgccgcg
     1861 gatcgggtac tcgagcacat aatcgcggcc gtcgatagtc cgcagctcct tgccttctgc
     1921 gagaggtgta ccaacgcccg tcgggcagaa gaacgccccg atgcccgctc cagccgcccg
     1981 catgcgctcg gccaggttgc cctgcggcac cacctcgagc gcgaccttgc ccgcgcggta
     2041 aaggccatcg aatacgtagg aatccgattg acggggaaag gagcagatca ctttgcggac
     2101 tcggcccgcc gccaacagtg cggcaaggcc ggtgtcaccg ttgcccgcgt tgttgctgac
     2161 gatggtcaga tcggtggcac cctgcgcgat gagcgcgtcg atcagcgtgg tgggcatacc
     2221 ggccatcccg aacccaccga cgagaaccgt tgccccgtcg gggattcccg cgaccgcctc
     2281 atcggcggta tcgcagatcg aggtgcgggt cacttgcctg cctcctgtgc gtcatagtcg
     2341 gcgagcacgc cgctggcgat ccggaatgcg gtattggcat ccggcacacc gcaatagatg
     2401 gccgtctgca gaatcacttc cttgatctcg tcgttggtca gcccgttgcg ccgcgcggcg
     2461 cgcacatgca tggccagctc ctcgtcgtga ccacgcgcga tcaacgccgt caaggtgatc
     2521 agcgaccggc tgcggcgatc gagcccggga
//
LOCUS       DASRJA010000490         5803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3471942,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000490 DASRJA010000000
VERSION     DASRJA010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3471942"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /gene="glmM"
                     /locus_tag="VFC01_21620"
     CDS             complement(<1..1089)
                     /gene="glmM"
                     /locus_tag="VFC01_21620"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZN82260.1"
                     /translation="MGRMFGTDGVRGVANRDLTAELALALGSAAARVGRAAHGGRRIA
                     VVGRDPRASSEMLEAAVIAGVTSEGVDALRVGVLPTPAVAYLTSAYDADFGVMISASH
                     NPMPDNGIKIFGPGGHKLDDAAEDRIEELVYQGPGSRPTGAGIGRVVDAQDALDRYLR
                     HAGKALTTRLDGITVVVDCAHGAASAAAPRAYRAAGANVIAINAEPNGLNINDRCGST
                     HMEQLQAAVTSHGADIGLAHDGDADRCLAVDAAGRVIDGDAIMVILALAMRDAGELAS
                     NTLVATVMSNLGLHLAMRAAGIEVRTTGVGDRYVLEELRAGEYSLGGEQSGHIVMPAL
                     GTTGDGIVTGLRLMSRMAQTRSTLAALAE"
     gene            complement(1249..1806)
                     /locus_tag="VFC01_21625"
     CDS             complement(1249..1806)
                     /locus_tag="VFC01_21625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82261.1"
                     /translation="MTSRLMRLLALVVSVAMIALVGALGTASTATAAPPGAGAKNTVQ
                     VTGTGPNGEKFTGTFTATGAQLDPDAPKGADILGTVTGQLTGGPGQPGPPKPVPATNA
                     AMPVADVQQATCQVLNLTLGPLDLSLLGLNVHLDQVHLVITATPGGGILGDLLCSLAG
                     GPGVPGTLDQVIALLQQIIGILQGL"
     gene            complement(2232..2645)
                     /locus_tag="VFC01_21630"
     CDS             complement(2232..2645)
                     /locus_tag="VFC01_21630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82262.1"
                     /translation="MQNRVKLASAGAVLGGALLAAGGFGLAHAAPPAESVVNDGKVNV
                     TVTAGGQQVGVLQDVSLTSAQALATSACPNAGITAEALQALDVNGTPVPSTCAGVDGG
                     LTFTFAQNGAVDSENVPGQSPASTMPSSSTTTTSR"
     gene            complement(3005..3400)
                     /gene="rpsI"
                     /locus_tag="VFC01_21635"
     CDS             complement(3005..3400)
                     /gene="rpsI"
                     /locus_tag="VFC01_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZN82263.1"
                     /translation="MVIDRPIQTVGRRKEAVVRVRLVPGTGQFHLDGRTLEAYFPNKV
                     HQQLIKAPLVTVDRLESFDIYAHLDGGGPSGQAGALRLAIARALILVQPEDRPALKKA
                     GFLTRDPRAIERKKYGLKKARKAPQYSKR"
     gene            complement(3475..3918)
                     /gene="rplM"
                     /locus_tag="VFC01_21640"
     CDS             complement(3475..3918)
                     /gene="rplM"
                     /locus_tag="VFC01_21640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZN82264.1"
                     /translation="MPTYTPKAGDTTRSWYVIDATDVVLGRLAVAAANLLRGKHKPTF
                     TPNVDGGDFVIVINADKVAISGDKLQNKYAYRHSGYPGGLRKRALGDEMEKHADRVVE
                     RAILGMIPHTKLGRQIQKKLKVYVGPDHPHTAQKPEPYEIKQVAQ"
     gene            complement(4094..4543)
                     /locus_tag="VFC01_21645"
     CDS             complement(4094..4543)
                     /locus_tag="VFC01_21645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82265.1"
                     /translation="MASSAGIASAPTAAADTTLALFEHDTVQHQADLGGPGPGPGDQF
                     VFAGDVFDRAGGMLLGTTGGSCTTITGNDKSGQTSCNGTFNLAGGQIVVQGVVDTAAL
                     FVRNETVPLSIVGGTGMYQNASGDGTVQLVAGVPNLTDANFVLNLAG"
     gene            complement(4619..>5803)
                     /locus_tag="VFC01_21650"
     CDS             complement(4619..>5803)
                     /locus_tag="VFC01_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82266.1"
                     /translation="WWTSAGTDLRAVAMAAQLNLAVAVLVRQQYAINALAWLVTRPPT
                     WWPLRIRWMLGKYYHFGGVHVGSAVAGTLWYLVLVGSLLHDAIAGGGTVSHAHVVLAA
                     TIVTAFAVMIVMALPRLRAAQHDRFEVTHRFCGWVALLLVWVNTMLFVNVHRGDQSFG
                     AALLAAPSFWLVALTAGLAIWPWLLLRRVPISYVRPSTHAVILRLDHGVTPGIGTTRP
                     ISRHPFVGWHHFANVPAAPGAAGYRMVVSRAGDWTAAFVDEPPSHIWVRGIPTMGVAN
                     VRKLFTKVVLVATGSGIGPMLGHLLDTSVPARLVWVTKNPRRTYGDQLVDEIEAAHPD
                     ATFWDTGERGKPDVLQLAYSAYLESGAEAVVCIANRTVTWQVVHGLESRGIPAFGPIW
                     DS"
BASE COUNT         1005 a         1893 c         1898 g         1007 t
ORIGIN      
        1 ctcggccagc gccgccaagg tcgagcgcgt ctgcgccatc ctcgacatca gccgcaagcc
       61 ggtgacaatg ccgtctcccg tcgtgccgag cgccggcatc acgatgtggc ccgactgttc
      121 cccgccgagc gaatactcgc ctgcccgtag ctcttcgagc acgtagcggt cgccgacccc
      181 ggtggtgcgg acctcgatgc ccgccgcgcg catggccaga tgcaggccga ggttgctcat
      241 caccgtggcc accaatgtgt tggatgccag ctccccggcg tcgcgcatcg cgagcgcaag
      301 gatcaccatg atcgcgtcgc cgtcgatgac ccgtccggcg gcgtccactg ccagacaccg
      361 gtcagcatcg ccgtcatgcg cgagcccgat gtcggcgccg tgcgacgtca cggccgcctg
      421 cagctgctcc atgtgtgtag acccgcagcg gtcgttgatg ttcagcccgt tgggctcggc
      481 gttgatcgcg atgacgttcg cgcccgccgc gcggtatgcc cgcggcgcag cggcggatgc
      541 ggcaccgtgc gcgcagtcga cgaccacggt gatcccgtcg agccgcgtgg tgagcgcctt
      601 gccggcgtgg cgcaggtagc gatccagcgc gtcctgggcg tcgacgacac ggccgatgcc
      661 cgcgccggtc ggccggctgc ccgggccctg gtagacgagc tcttcgatgc gatcctctgc
      721 ggcgtcgtcg agcttgtgtc cgcccggccc gaagatcttg atgccgttgt ccggcatcgg
      781 gttgtgcgac gccgagatca tcacgccaaa gtcggcgtcg tatgcgctgg tcaggtacgc
      841 caccgccggc gtcggcagaa caccgacgcg cagcgcgtcg accccttcac tggtcacgcc
      901 ggcgatcacc gccgcctcga gcatctcgct gctggcccgc ggatcgcggc cgaccacggc
      961 tatgcgacga ccgccgtgag ctgctctgcc gacccgcgcc gcggcggaac ccagtgccag
     1021 cgccagttcg gcagtcagat ctcgattcgc gaccccacgg acaccgtcgg tgccgaacat
     1081 ccgacccatg cggacaaact tctcacaatt cggccgacga aacacaaccg acgaggtgat
     1141 cggctatccc ccagccgaac tggtacccga tcgagaccca gacaagcggc accgccccac
     1201 gcttggcgta agggcggtgt cgtcggggtt aactagcggt tctggacttt atagtccttg
     1261 gaggatcccg atgatctgtt ggagcaaggc gatgacctga tccagcgtcc ctggcactcc
     1321 tggcccaccg gcgaggctgc acaacaggtc gccgagtatg ccaccgcctg gggtcgccgt
     1381 gataaccaaa tgaacttggt ctagatggac gttcaggcct agcaggctca ggtcgagcgg
     1441 cccgagcgtc aggttgagga cctgacatgt tgcctgctgg acgtcggcga cgggcatcgc
     1501 agcgttggtc gcggggacgg gcttaggcgg tccaggttgg ccgggtccgc ctgtgagttg
     1561 accggtcact gtgccgagga tgtccgcgcc ctttggagcg tccggatcga gctgggcacc
     1621 ggtagccgta aaggtaccgg tgaatttctc tccgttagga ccggtaccag tcacctgcac
     1681 ggtgttcttg gcgcccgcgc caggtggcgc ggccgttgcg gtgctcgccg tgccgagcgc
     1741 gccgacgagg gcgatcatcg cgacgctcac cactagggcg agcagtcgca ttagacgtga
     1801 cgtcatgcgg atactcctct tctaatatcg aggaagcagt gatccggccc cccgaccagc
     1861 tccatgacgt cgcgccggag gcgggaatgg cccgccctgc gccgaaggca cccctgcttc
     1921 ctcggggttt ctttcctcga tcagagggca gttcaaacca aaagttagcg agaaaattag
     1981 acgggtttgc cattacggcc ttaagctaat tagctatagc gttaataatt tgctacaaat
     2041 ccgcagatca gcccatctac tagtacgtcg ctgctccccg ccagaggtta ctggttcgac
     2101 tgaaaaattt cttcggcgtc aacacaaggt gtacatgaaa cgaagtgcgc gcattcaccc
     2161 agcgaggccg aagacgaagc accgcccgca tccgaccgat acgggcggcg agcttagtcg
     2221 ttgaccgaga actaccgcga ggtagtggtc gtcgaactcg acggcatcgt tgaggcgggg
     2281 ctctgacccg ggacgttctc cgagtcaacc gcgccgttct gagcgaacgt gaaggtcaga
     2341 ccgccgtcaa cgcccgcgca tgtgctgggc accggcgtgc cgttgacgtc gagcgcctga
     2401 agcgcctccg cggtgatccc ggcgttgggg cacgcagagg tggcgagcgc ctgcgcgctc
     2461 gtcagcgaca cgtcctggag gacgccgacc tgctggccgc cggccgtcac ggtgacgttg
     2521 accttgccgt cattgacaac cgattcggcc ggtggagctg cgtgcgcgag gccaaagccg
     2581 cccgcggcca gcagcgctcc acccagtacg gcgccagccg acgcaagctt cacgcggttc
     2641 tgcatcagtt attccctttc tgtcaggaag cattgatccg gcccacgacg tgcatagcag
     2701 tcaccggtgg cagggtctgc ctgcctggct cgaaaaggtg cccgtgcttc cggcggaggg
     2761 acttccacgt tcgacggtga actcaaacca aatttaagaa ttggaagaca attcacgcct
     2821 accgtatcga ctcactaatt agccatcgca ccaatgcctt tggcgtcgac gcactaaagg
     2881 acgttccatg cccgcgaata aaagttactg ggtgcttcgc aaaaatctgc gtcccaacgc
     2941 aattctgtcc agaaagcgaa acgccgggcg tgcgtatcgc acacccggcg tttcgataga
     3001 tcgatcagcg cttgctgtac tgaggcgcct tgcgggcctt cttgaggccg tacttcttgc
     3061 gctcgatggc ccgcgggtcg cgggtcagga agccggcctt cttcagcgcg ggccggtcct
     3121 cgggctgcac caggatcaga gcccgcgcga tggccaggcg cagcgcgccg gcctgaccgg
     3181 acgggccgcc gccatcgagg tgggcataga tgtcgaagct ctccagccgg tccacggtca
     3241 ccagcggagc cttgatgagc tgctggtgga ccttgttcgg gaagtaggcc tcgagggtgc
     3301 ggccgtccag gtggaactgg ccggtgccgg gcaccaggcg cacccgcacc acggcctcct
     3361 tgcggcgacc gacggtctga atgggacggt cgatgaccac gggttcgcgc ggagcggcct
     3421 cggtctcggt ctcggtctcg acgacggtct cggtggtcgt ctctacggtc tcggtcactg
     3481 ggccacctgc ttgatctcgt acggctccgg cttctgggcg gtgtgcggat ggtcgggccc
     3541 gacatagacc ttcaacttct tctggatctg acggccgagc ttggtgtgcg gaatcatgcc
     3601 gaggatcgcc ctctcgacga cccggtcggc gtgcttctcc atctcgtcac cgagcgcacg
     3661 cttgcgcaga ccgccgggat aacccgagtg gcggtaggcg tacttgttct ggagcttgtc
     3721 gccgctgatg gcgaccttgt ctgcattgat gacgatgacg aagtcgccac cgtcgacatt
     3781 cggcgtgaat gtcggcttgt gcttgccgcg cagcagattc gcagccgcga cggcgagccg
     3841 gccgagcacc acgtcggtgg cgtcgatgac gtaccacgac cgcgtggtgt cacccgcctt
     3901 cggcgtgtat gtaggcacag cgtctacctt ctcttctcgg gttggatccc ggtgtgaccc
     3961 gggtgccggt caggcgttca cggcagggtg attctcggcg accgacgttg acccgaggac
     4021 ccgtgcatac cgcacgccaa cgacgcagct tacctgcggg tgtcctcgca ggtcaaaacg
     4081 cgttcattga gggctaaccg gccaagttca ggacgaagtt cgcatcggtc agattgggga
     4141 cacccgcgac caactggacg gtgccgtcgc cgctggcgtt ctggtacatc ccggtgccgc
     4201 cgacgatcga tagcggcacc gtctcgttgc ggacgaacag ggcggccgtg tcgaccacac
     4261 cctggaccac gatctggcca ccggcgaggt tgaatgtgcc gttgcacgac gtctggccag
     4321 acttgtcgtt gccggtaatc gtggtgcaac tgccgccggt tgtgcccagg agcatgccac
     4381 cggcgcggtc gaacacatcg cctgcaaaga cgaattgatc gcccgggcct ggtcccggcc
     4441 cgccgaggtc cgcctggtgt tgcacggtgt cgtgctcgaa gagagccagc gtggtgtcgg
     4501 cggccgcagt cggcgccgac gcgatccccg cagagcttgc cacggcaaca gctagagcaa
     4561 gttttgcgta gttattcata tcgccccccg atattgcgaa ttagctgaaa cgtacgcctt
     4621 acgagtccca gatgggcccg aatgcgggga tcccgcggct ctcgagaccg tgcaccacct
     4681 gccacgtcac ggttcggttg gcgatgcaca cgacagcctc ggcgcccgat tcgaggtagg
     4741 cggagtacgc cagctgcagg acatcgggct tcccgcgctc accggtgtcc cagaaggtcg
     4801 cgtcgggatg ggccgcctcg atctcgtcta cgagctggtc gccgtaggtg cggcggggat
     4861 tcttggtgac ccagaccagc cgcgcgggga cggaagtgtc gagaagatgc cccagcatgg
     4921 ggccgatccc gctgccggtg gcgacgagaa cgactttcgt gaacagcttc cggacattgg
     4981 ccacgcccat cgtggggatg ccgcgcaccc atatatgtga cggcggctcg tcgacgaacg
     5041 cagcggtcca gtcgcccgcg cgggagacga ccatgcggta gccggcagcg cccggggcag
     5101 cgggcacgtt ggcgaagtga tgccaaccga cgaatgggtg acgactgatc ggcctggtgg
     5161 tcccgattcc cggggtgacg ccgtggtcga gccgcaggat cactgcatgc gtcgacggtc
     5221 gcacgtagct gatcggcacc cggcgcagga gcagccacgg ccagatcgcc aatcctgcgg
     5281 tcagcgcaac cagccagaag gacggcgctg ccaggagcgc ggcaccgaag gattggtcgc
     5341 cgcggtggac attgacaaac agcatcgtgt tgacccacac cagcagcagc gcgacccagc
     5401 cacagaaccg gtgggtcacc tcgaagcggt cgtgctgcgc ggcgcgcagt cgcggcagcg
     5461 ccatgacgat catgacggcg aaggcggtga cgatagtggc ggcgagcacc acgtgggcgt
     5521 gagagaccgt gccgccgccg gcaatcgcgt cgtgcaggag tgacccgacg aggaccaggt
     5581 accacaaggt tccggccacc gcgctgccga cgtgcacgcc accgaagtgg tagtacttgc
     5641 cgagcatcca tctgatgcgc agcggccacc aggtcggggg ccgagtcacc aaccaggcga
     5701 gcgcattgat ggcgtattgc tgccgaacca gaacggccac agcgagattc aattgcgcgg
     5761 ccattgcgac ggcgcgcaga tcggtgcccg cgctcgtcca cca
//
LOCUS       DASRJA010000491        10578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1423582,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000491 DASRJA010000000
VERSION     DASRJA010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10578
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423582"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VFC01_21655"
     CDS             complement(<1..142)
                     /locus_tag="VFC01_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN82267.1"
                     /translation="MTIADKAVLVTGANRGIGRALVEEALTRGAKRVYAATRQPVAHP
                     DAR"
     gene            305..1315
                     /locus_tag="VFC01_21660"
     CDS             305..1315
                     /locus_tag="VFC01_21660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN82268.1"
                     /translation="MVTTDLIARARAGDGDAFRELTEPHRRELLVHCYRMLGSFHDAE
                     DALQDALLAAWQALDGFEGRASIRTWLYRIATNRCLDALRSAKRRPAKEWDISEIEMP
                     EPTRLGEITWLEPFPDVFLEGTPGTPLGPEARYEQTEAISLAYVTALQVLPARQRAVL
                     ILREVLGYHADEVADMLDSTVDSVNSALKRARATLQKRLASSPGHESPPTPDSPAERA
                     LVAKFVRAYESGDVNGLVALLTADVCVSMPPIPLEYQGRDVVARFYASVFRKGGRYDL
                     VPTRANDQLAFGAYLRTPTGGIRHGAGLLVLTVAGGRISALTRFDNDMLPSFGLPRSL
                     PG"
     gene            complement(1373..1786)
                     /locus_tag="VFC01_21665"
     CDS             complement(1373..1786)
                     /locus_tag="VFC01_21665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN82269.1"
                     /translation="MPPIGPSPTNTLDVLLHQNPMIAANSCAMVATVTIPPGDPGTGP
                     HRHSGPVFGYVLEGEILFELEGEEPYSIKAGEAFWEPGFDVVHYQAANLRDDIASKFV
                     VFMWCAPDMPMMTFLDEEEINARAHLRYQSSQPRA"
     gene            1907..2374
                     /locus_tag="VFC01_21670"
     CDS             1907..2374
                     /locus_tag="VFC01_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82270.1"
                     /translation="MAEEPRTTPLMPDDFVVPTELRSTSFRLEPLGPDHNARDHEAWM
                     SSIAHLQTTPGFEDRGWPPDEGFSAERNLADLEGHADEFERRVAFAYSVLDPNSDDVI
                     GCVYIDPGDRDGSASVRSWVRADVAELDVPLRRAVRDWLTESWPFNDIEYAGG"
     gene            complement(2397..3497)
                     /gene="dapC"
                     /locus_tag="VFC01_21675"
     CDS             complement(2397..3497)
                     /gene="dapC"
                     /locus_tag="VFC01_21675"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HZN82271.1"
                     /translation="MRKRVSALLPVFPWDTLADVTALARSHPDGIVDLSVGTPVDPVA
                     PLIRDALAAASSAPGYPTTAGTPALRASAVAALSRRYGITGLADDAVVPVIGTKELIA
                     WLPTLLGLGPDDLIVVPELAYPTYEVGAQLAGARVLLADSLTQLGPQSPALIYLNSPS
                     NPTGKVLGVDHLRKVVGWARERGALVASDECYLGLGWDAQPYSVLHPDVCDGDHTGLL
                     AVHSLSKTSSLAGYRAGFVAGDPAVVAELLAVRKHAGMMVPTPVQAAMVAALDDDAHE
                     REQRERYERRRAALLPALQSAGLTVEDSEAGLYIWGTRGEPCRDTVAWLARRGILVAP
                     GEFYGPRGSQHVRVALTATDERIAAAASRLRG"
     gene            complement(3512..3838)
                     /gene="fdxA"
                     /locus_tag="VFC01_21680"
     CDS             complement(3512..3838)
                     /gene="fdxA"
                     /locus_tag="VFC01_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN82272.1"
                     /translation="MTYTIAEPCVDIKDKACIEECPVDCIYEGARMLYIHPDECVDCG
                     ACEPVCPVEAIYYEDDVPDQWSGYTQINADFFAELGSPGGASKVGLTENDPQSVKDLP
                     PQGEDS"
     gene            4212..4424
                     /locus_tag="VFC01_21685"
     CDS             4212..4424
                     /locus_tag="VFC01_21685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82273.1"
                     /translation="MKKFALSVAGAGVFAAVTLGLATPAFATSDYVVPGHDAVYSSQT
                     DSATIRPVDCSVHVTSGGTDVNVNWC"
     gene            4506..5063
                     /locus_tag="VFC01_21690"
     CDS             4506..5063
                     /locus_tag="VFC01_21690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZN82274.1"
                     /translation="MALPHAILVSLCEQSGSGYELARRFDRSIGYFWAATHQQIYRTL
                     KSMEDDGWVVVTPVVQQGRPDKKVYTVSDEGRAELARWIAEPLSGRGSSVADNRTRDI
                     AIKVRGAAYGDVDAVRAQVASLRSERAELLDIYRGFEKRQFPDPGDLTGSALHQYLVL
                     RGGVRAEESAIDWLDEVAAALKGAP"
     gene            5060..7066
                     /locus_tag="VFC01_21695"
                     /pseudo
     CDS             5060..7066
                     /locus_tag="VFC01_21695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
     gene            7131..7538
                     /locus_tag="VFC01_21700"
     CDS             7131..7538
                     /locus_tag="VFC01_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82275.1"
                     /translation="MTCFAIDATTLLHLVACGRRADPAHQLVAPNLIRSHALTLLLQS
                     VLRGELTDAEALERHERITEVKIRLLGDRVSRRTAWKLAREHGWDSIYGAEYIAIAKL
                     QADQLVTVDPELTAMATGIVPLAPFNRLAGGQR"
     gene            complement(7549..7869)
                     /locus_tag="VFC01_21705"
     CDS             complement(7549..7869)
                     /locus_tag="VFC01_21705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82276.1"
                     /translation="MVGKVLLATSLAAGGMFVGGVAHAENVMNLGTYAGDVIEQLQDW
                     GYTVMLNGLDRDIAYMDETHKRHCQVLGIHPVVSEPLADGEFQTVFVDLSCPGNPSSS
                     TPSP"
     gene            8037..8597
                     /locus_tag="VFC01_21710"
     CDS             8037..8597
                     /locus_tag="VFC01_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZN82277.1"
                     /translation="MTSTFDATGTTQLSAGTWAIDPVHSSIGFSVRHLMVSKVRGNFE
                     KFSGAIVVAEDGTPSVTAEIAVDSIHTNNEQRDGHIKSADFFEVEKYPTATFRSTGVR
                     GNGDNYVVDGEFTLKGVTKPVSLDLEFNGVNPGMGHGEVAGFEASVVLNRKDFGIDID
                     MPLETGGTVVGDKVTITLEIEALKQS"
     gene            complement(8669..9130)
                     /locus_tag="VFC01_21715"
     CDS             complement(8669..9130)
                     /locus_tag="VFC01_21715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HZN82278.1"
                     /translation="MTTIAGLPAHALLVHAIVVLAPLTALLEILCAFWPAARSRLVWL
                     VLAFAAVNLVLTPLTTNAGEWLLSQRSSVSAVLQTHAERGDWMIYFSVALFVVAVALA
                     VLHWLAGRSDRRRTVANVVVAIVALLVGVSSIITVVRIGDSGAHSVWGGGG"
     gene            complement(9180..>10578)
                     /gene="cofH"
                     /locus_tag="VFC01_21720"
     CDS             complement(9180..>10578)
                     /gene="cofH"
                     /locus_tag="VFC01_21720"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HZN82279.1"
                     /translation="TAQPKYVQAGAAWIDPRVRGHVDALADPDTGLAADVNPVGRPWQ
                     EPDEAAESLGRIDLATAIDSEGRITETRSDLDSAFGDWESIREKVHELAARAPERIDT
                     DVLAALRSAERDPAGCSDDEYLALATADGPALDAVAALADSLRKDVVGADVTFVVNRN
                     INFTNICYTGCRFCAFAQRKGDADAYSLSTAEVADRAWEAHVAGATEVCMQGGIDPEL
                     PVTGYADLVRAVKARVPSMHVHAFSPMEIANGVTKRGISIREWLTDLREAGLGSIPGT
                     AAEILDDEVRWVLTKGKLPTSMWIEIVTTAHEVGLRSSSTMMYGHVDAPHHWVGHLNV
                     LRGIQDQTGGFTEFVPLPFVHQSSPLYLAGGARPGPTHRDNRAVHALARIMLHGRISN
                     IQTSWVKLGVERTQVMLQGGANDLGGTLMEETISRMAGSEFGSAKSVEELVEIAAGIG
                     RPARQRSTTYAPLAA"
BASE COUNT         1891 a         3517 c         3361 g         1809 t
ORIGIN      
        1 cgcgggcgtc cgggtgtgcc acgggttgac gcgttgcggc gtacacccgc ttggcgcctc
       61 gagtcagggc ttcctctacc agtgcccgtc cgattccgcg gttagccccg gtcaccagaa
      121 ctgccttgtc tgcgatggtc atgcatcctc ctagctgatg atttcgagct gtcgaagatg
      181 taggcgctgc aacactgaga aattggtcgc ggattttgcg cccaattcgc tgcccctgcg
      241 acgtcgatat ttgaaagtcg acgaggagag cgagagacgt gacgcgaggg tggagtggtg
      301 acacgtggtg acaaccgacc tgatcgccag ggcgcgagcc ggggacggcg acgcgttccg
      361 agagttgacc gagccacacc gtcgagagct gctggtgcat tgctatcgga tgctcggctc
      421 gttccacgac gcggaggacg ccctgcagga cgcgttactc gcggcctggc aggccctcga
      481 tggattcgag ggacgcgcct cgatccgcac ctggctctac cggatcgcca ccaaccggtg
      541 cctcgacgcg cttcgctcgg cgaagcgacg cccggccaag gagtgggaca tctccgagat
      601 tgaaatgcca gagccgactc gactgggcga aatcacgtgg ctggaaccat ttcccgatgt
      661 gttcctcgag ggcacgcccg gaacgcccct cggtcccgag gcccgctacg agcagaccga
      721 ggccatctcg ctggcctacg tgaccgcgct gcaagtcctt ccggcccgcc aacgcgcggt
      781 gctcatcttg cgtgaggtgc tcggatacca cgccgacgag gtggcggaca tgctggactc
      841 gaccgtcgac tcggtcaaca gcgcgctcaa acgcgcccgt gccacgctgc agaagcgcct
      901 agcgtcgagc cccgggcacg aatcgccacc cactcctgac tcacccgccg agcgggcgct
      961 ggtggcaaaa ttcgtgcgcg cgtatgagtc tggcgatgtc aatgggttgg tcgccttgct
     1021 caccgccgat gtctgcgtgt ctatgccacc gatcccgctc gaatatcaag ggcgcgacgt
     1081 cgtggcccgc ttctatgcga gcgtgttccg caaaggcggc aggtatgacc tcgtgccgac
     1141 gcgggccaat gatcagctgg cgttcggggc gtaccttcgc acgcccaccg gcggaatccg
     1201 ccacggagcc ggcctgctcg tcctgaccgt cgctggcggc cggatctccg ccctgacccg
     1261 cttcgacaac gacatgctcc cgtcgttcgg gctgccgcga tcgcttcccg gctgaggacc
     1321 atggcctgga cgtcgtaatc gctcaagcgg caaccgtttt gccggttcgg tatcaggcgc
     1381 gcggttgtga tgactggtag cgcagatgcg cgcgggcgtt gatctcttct tcgtcgagga
     1441 aggtcatcat cggcatgtcc ggcgcgcacc acatgaacac cacgaacttg cttgcgatgt
     1501 cgtcgcgcag gttcgcggcc tgatagtgca cgacgtcgaa acccggttcc caaaacgcct
     1561 ctccagcctt gatcgagtag ggctcctcgc cttcaagctc aaagaggatc tcgccttcca
     1621 acacataacc gaacaccggg ccggagtggc ggtgtggccc tgtgccgggg tcgccgggcg
     1681 gaatggtgac agtggcgacc atcgcgcacg agttcgcggc gatcatcgga ttctggtgca
     1741 gcagaacgtc cagcgtgttg gtcggtgatg gcccgatcgg cggcatgtca gcgacggtaa
     1801 cgccgtcgcg tcggcgagcg ccggtccatt tctactcgga gtacgtgggc caaaccgtag
     1861 aactcgccgg gtcgcagccc cggcaacgag gtgcaaaggt gaaggcatgg cagaagagcc
     1921 gagaaccacc ccgctgatgc ccgatgactt cgtcgttccg acggaacttc gatccacgag
     1981 cttccggctc gaaccgctcg gcccggacca caacgccaga gatcacgagg cgtggatgtc
     2041 gagcatcgcc cacctgcaga ccacaccggg cttcgaggat cggggctggc cgcccgacga
     2101 gggattctca gcggagcgga acctcgccga tctggagggg cacgcggacg aattcgagcg
     2161 gcgcgttgcg ttcgcctact cggtgctgga tcccaattcc gacgatgtca tcggctgtgt
     2221 atacatcgac cccggcgacc gggacggcag cgccagcgtt agatcctggg ttcgtgccga
     2281 cgtggccgag ctcgacgttc cgttacgtcg ggccgttcgt gactggctga cggagtcttg
     2341 gccattcaac gacatcgagt acgcaggcgg gtgaacacct ttcggcgccg gcgatatcag
     2401 ccgcgcaacc tggacgccgc agcggctatc cgctcatccg tcgccgtcaa cgcgacccgg
     2461 acatgctgcg aaccgcgcgg gccgtagaac tcgcctggcg ccaccagaat cccccgccgc
     2521 gccagccacg ccactgtgtc gcggcacggt tcaccgcggg tcccccaaat gtaaaggccg
     2581 gcctcggagt cctcgaccgt cagcccggcc gattgcagcg caggcagcag tgccgcccgt
     2641 cgccgctcat accgctcgcg ctgctcgcgt tcgtgcgcat cgtcgtcgag cgccgcaacc
     2701 atggccgcct gcaccggcgt cggcaccatc atccccgcgt gtttacgcac cgccagcagt
     2761 tcggcgacca ccgccgggtc gcctgcgacg aaccccgcgc ggtagccggc cagcgacgag
     2821 gtcttcgaca gcgaatgcac cgccaacaat ccggtgtggt cgccgtcgca gacgtcgggg
     2881 tgcagcaccg agtacggctg ggcgtcccac ccgagcccca gatagcactc atcggatgcg
     2941 accagcgcgc cgcgttcgcg ggcccaaccc accaccttgc ggagatggtc gacgcccaac
     3001 acctttcccg tcgggttgct cggcgaattc agatagatca acgcgggcga ctgcgggccg
     3061 agctgcgtca gcgaatccgc caacagcacc cgagcgcctg ccagttgagc gccgacttcg
     3121 taagtgggat aagccagttc gggcaccacg atgaggtcgt ccgggccgag accgaggagc
     3181 gtcggcagcc acgcgatcaa ctccttggtc ccgatcacgg gaaccacggc atcgtcggcc
     3241 agtccggtga tgccgtaccg ccggctcagg gccgcgactg ccgacgcgcg caacgccgga
     3301 gtgccggctg tggtcgggta gccgggcgcg gagctggccg cggccagcgc gtcccggatc
     3361 agcggcgcca ccggatcgac cggcgtgccc accgacaggt cgacgatgcc gtcggggtgc
     3421 gagcgggcga gcgcggtgac gtcggccagg gtgtcccagg ggaacaccgg caataacgcc
     3481 gagacgcgct tacgcagctg cgggcgagcc gttaggagtc ctcaccttga ggcgggagat
     3541 ccttgaccga ctgcggatcg ttctccgtca gccccacctt ggaggcaccg ccgggcgagc
     3601 ccagttcggc gaagaagtcg gcgttgatct gcgtgtatcc gctccactga tcggggacat
     3661 catcttcgta gtagatggcc tcgactgggc agaccggttc gcatgcgccg cagtcgacgc
     3721 actcgtccgg gtggatgtac agcatgcgtg cgccctcgta gatgcaatcg actgggcact
     3781 cttcgataca tgccttgtct ttgatgtcga cgcagggttc ggcaatcgtg tacgtcactg
     3841 aggtctcctc gatctcctgt cagtggggct gccgctcttt ggtcgggccc gccgaaaagt
     3901 cgctgcaagt atgcgttgcg gttacttggt cgcctgtctc agtgtgccct aacttccacg
     3961 tctcccggtt aagggttccg catggttact gatactagac gttgcacata cagtgcgcct
     4021 agggacaccc gcacatgact ccgcgttcat gctgctctac gcccatgtga cgccgaccac
     4081 atgggttgcg cgcatgtgat cacttgcgag ccggggcgaa atgacccgcg tgacggaaca
     4141 ccggcgtcgc gtaacgggtt ttaccgaata ccaagtactt agcttgtcta cgtccgacaa
     4201 aggagtcaga aatgaagaaa ttcgccctca gcgtggccgg tgccggcgtg ttcgccgcgg
     4261 tcaccctcgg tttggccacc ccggccttcg cgacatccga ctatgtcgtc cccggccacg
     4321 acgccgttta ctcgtcgcag accgactccg cgaccatccg gccggtcgac tgctcggtgc
     4381 acgtcacctc cgggggcacc gacgtcaacg tgaactggtg ctgacctgac ggacccgcgg
     4441 cctatgcgct tcgttgcagt gcaacaagtt gcatagtgcc gcgggttcgc catatgctgc
     4501 cccgcgtggc gctcccccac gcgatcctgg tgtcgctgtg cgaacagtcc ggctccggtt
     4561 atgagctggc ccgccggttc gaccggtcca tcggctactt ctgggcggcc actcatcagc
     4621 agatctatcg gacgctcaag tcgatggagg acgacggttg ggtggtcgtc acgcccgtcg
     4681 tgcagcaggg caggccggac aagaaggtct acaccgtctc ggacgagggc cgcgccgagc
     4741 tggcgcggtg gatcgccgaa ccgctgtctg gccgcggcag ctccgttgcc gacaaccgca
     4801 cccgggacat tgcgatcaag gttcgcggcg ccgcctacgg cgacgtcgat gccgtgcgag
     4861 cacaggtggc ctccctgcgg tccgagcgcg ccgaattgct cgacatttac cgcggattcg
     4921 agaagcggca gtttcccgac cccggcgatc tgactggcag cgcgctgcac caatacctgg
     4981 tgctgcgcgg cggtgtccgc gcggaggaaa gcgccatcga ctggctcgat gaagtggcag
     5041 cagcattgaa gggggcacct tgaactatcc gaagttgttg tcgccgttgg accttggctt
     5101 taccacgttg cgaccgggtc gtgatgggtt cgatgcacac gggtctcgag gaccgcgccc
     5161 gcgacaccga ccggctcgcg gcgtatttcg ccgagcgcgc ccgcggaggc gtcggcctga
     5221 tcatcaccgg cggctatgcg ccgaaccgca ccggctggct gctgccgttc gccgcgcaga
     5281 tgacgtcgtc atcggatgcc cgccggcatc gccgcattac ccgcgccgtg catgacgccg
     5341 acggcaagat ccttctgcag atcctgcacg cgggtcgcta tgcgtaccac ccgttttcgg
     5401 tgagcgcatc gtcgatcaag gcgccgatca acccgttccg tccgcgggcg ttgcgtgatg
     5461 tcgatggcac catcgacgac ttcgcccact gcgccgagct cgcccgcgag gcgggatacg
     5521 acggtgtcga gatcatgggc aacgagggct acctgctcaa ccagttcctc gccccgcgga
     5581 ccaacaagcg caccgacgcc tgggcggctc gccggagaaa cgtcgccgtt tccctgtcga
     5641 gatcgtgcgt cgcacccgcg ccgcggtcgg cgacgacttc atcatctgct accggatgtc
     5701 aatggccgac tacgtcgagg acggtcagag ctgggacgaa atcatcgcgc tggcaaccga
     5761 agtcgaggca gcgggcgcaa cgattatcaa caccggcatc ggatggcacg aggcgcgggt
     5821 accgaccatc gtcacctcgg tgcccaacag cgcgttcgtc gacatcagca atgcggtggc
     5881 cgaacacgtg acgatcccgg tggtcgcgtc caaccgcatc aacatgccgg aagccgccga
     5941 acagattctc gccgacggca acgtgcagct gatctcgatg gcgcgcccgc tgctgtcgga
     6001 cccggactgg atgctcaagg tgcaggccga cgccgccgac gagatcaaca cctgcatcgc
     6061 ctgcaaccag gcctgcctgg accacgcatt cgtgcacaaa acggtgtcgt gccttctcaa
     6121 tccgcgagcg ggccatgaga ccaagttggt actcggtccg acgcggcgca ccaaacgcgt
     6181 ggccgtggtg ggcgcgggac cggccggtct cgcgaccgcg gtcaccgctg cacagcgcgg
     6241 acacaacgtc acactgttcg aagcgagcga cgcgcttggc ggccaattcg atttggcgag
     6301 aaggattccc ggcaaggagg agttcagcga gacgatccgc tactacacgc ggatgctcga
     6361 caagcacggc gtcgatgtgc agctgcgtac tcgcgcgacg gtcgacgaac tagccggctt
     6421 cgacgaggtc gtgctggcga ccggcgtttc gccgcggatg cccgccatcc ccggcatcga
     6481 ccatccgatg gtgctgtcct acgccgaggc gatcatgggc aagccggtcg gcaagtcggt
     6541 cgcggtggtc ggcgcgggcg gggtcggatt cgacgtcagt gagttcctgg tgactgacga
     6601 gtcgccgacg ctgaacctca aggagtggaa ggccgaatgg ggcgccgcgg atccgcagga
     6661 ggtgcgtggc gcgttgacga cgccgatccc ggcgccgcct gcgcgtgagg tgtatctgct
     6721 gcagcggacg aaaggtccac agggcaagcg gctcggcaag acgtcgggct gggtgcaccg
     6781 cgcatcgctg aaagccaaag gcgtgcagca gttgtccggc gtgaattacg aaagaatcga
     6841 tgacaacggg ttgcacatca gcttcggccc ggatcgctcc gacccgtgcc tgctcgaggt
     6901 cgacaacatc gtgatctgcg ccggccagga ctcggtacgg gacctcgaag agggcctacg
     6961 ccgaaacggc atcgagccac acgtcatcgg cggggccgct gtagccgccg aactcgacgc
     7021 caagcgcgcc atccggcaag gtaccgagct ggcggccagg ctttagcctg cctggtgcgc
     7081 cagcatgcat tcttacgact tcgagcgtcg acgctttcta ctgtgactgc atgacctgct
     7141 tcgcaatcga tgccacgacc ctgttacacc tcgtggcttg tggacgacgc gccgacccgg
     7201 cacaccaatt agtggcaccg aacttgatcc gatcgcacgc actcacgctg ctgctacaga
     7261 gtgtgctccg cggagaactc accgacgccg aagcccttga gcgacacgag cgcatcaccg
     7321 aagtgaagat tcgcttgctg ggcgatcgag tgtcccgtcg caccgcatgg aagctggctc
     7381 gtgagcacgg ctgggacagc atttatggcg cggaatacat cgcgatcgcc aaactgcaag
     7441 ccgaccaact ggtcaccgtt gatccagagc taaccgcgat ggcgaccgga atcgtcccgc
     7501 tcgcgccctt caaccgccta gctggcggcc aacgttagga cgaaactctt agggcgacgg
     7561 tgtcgagctg ctggggttgc ccgggcacga caagtcaacg aaaaccgtct ggaactcgcc
     7621 gtcagctagg ggttcactca ccaccgggtg aatgcccagc acctggcaat gtctcttatg
     7681 ggtttcgtcc atgtaggcga tatcccggtc gaggccattg agcatgacgg tgtagcccca
     7741 gtcctggagc tgctcgatga cgtcgccggc gtacgtcccg agattcatga cgttctcggc
     7801 gtgcgcgaca ccgcccacga acatcccgcc ggcagcgagg gatgtcgcca agagcacctt
     7861 tccgaccatc ggagttcccc cttgaactcg acctgaactg aaggtgaata caaggttaac
     7921 gcctgcgtca atttggtact agcgcgatgc cacggggaat gaattcggac tacggtccgt
     7981 ttagtccgtg gaaggcgtcg ggaaggccga cgcccctagg tgaaagagac atcgttatga
     8041 cctcaacctt cgatgcgacc ggaaccaccc agctcagcgc cggcacgtgg gccatcgatc
     8101 cggttcactc gtcgatcggc ttttcggtac gccatctgat ggtgagcaag gtgcgcggca
     8161 atttcgagaa gttcagtggt gcgatcgtcg tcgccgaaga cggcacgccg tcggtgacgg
     8221 ctgagatcgc cgtcgactcg atccacacca acaacgagca gcgcgacggc catatcaagt
     8281 cggccgattt cttcgaggtc gaaaagtacc ccactgcgac gttccggtcg accggcgtgc
     8341 gcggcaacgg cgacaactat gtggtcgacg gtgagttcac gcttaagggc gtcaccaagc
     8401 cggttagcct ggatctcgag ttcaacggcg tcaaccccgg catgggccac ggcgaggtgg
     8461 ccggcttcga agcgtcagtc gtgttgaacc gcaaggactt cggcatcgat atcgatatgc
     8521 cgctggaaac gggtggcacg gtcgtcggcg acaaggtcac gatcactctg gagatcgagg
     8581 cactcaagca gtcctgactc tctcgagtgt gaagccactg cgaacgatcc ggcgaaaact
     8641 cgcagtggct tcacactcgg tgcaggcgtc agcctccccc gccccatacc gaatgcgcgc
     8701 cggagtcacc gatccgcaca accgtgataa tcgatgagac gccgaccagc agcgctacga
     8761 tcgccaccac tacattggcc accgtcctgc gcctgtccga ccgccccgcg agccaatgca
     8821 gcacggccag cgcgacggcg acgacgaaca acgcgacgga gaagtagatc atccagtcgc
     8881 ctcgttcggc gtgcgtctgc aggaccgcac tcacgctgct tcgttggctc aacaaccact
     8941 caccggcatt ggtggtcaac ggggtgagca caaggttcac ggccgcaaag gccaggacca
     9001 gccagaccaa ccggctccgg gccgccggcc agaacgcgca caggatctcg agcaatgcgg
     9061 tcaatggcgc gagcacgacg atcgcgtgca cgagcaaggc gtgggcaggc agtccagcga
     9121 tcgtcgtcat cagcgccaca gtattcgtga atagctgaaa acttgctgtt aatcgtgagc
     9181 taggcggcca gcggcgcgta ggtcgtcgac cgctggcggg cgggcctgcc gatgcccgcc
     9241 gcgatctcga ccagttcttc gacgctcttg gccgagccga attccgagcc cgccatccgc
     9301 gagatcgtct cctccatcag cgtcccgccg aggtcgttgg caccgccctg cagcattacc
     9361 tgcgtgcgct cgacgccaag cttcacccag ctggtctgga tgttggagat cctgccgtgc
     9421 aacatgattc gcgccaaggc atgtactgcg cggttgtcgc ggtgcgtcgg ccccgggcgc
     9481 gcgccacccg cgagatagag cgggctgctc tggtgcacga acggcaatgg cacaaactca
     9541 gtgaacccac cggtctgatc ctggatgccg cgcagcacgt tgagatgccc gacccagtga
     9601 tgtggcgcgt cgacgtggcc gtacatcatc gtcgacgacg accgcagccc gacctcgtgc
     9661 gcggtggtga cgatctcgat ccacatcgac gtcggcagtt tgcctttggt caacacccac
     9721 cgcacctcgt cgtcgaggat ctcggcggcc gtgccgggga tgcttccgag tccggcttca
     9781 cgcagatcgg tcaaccactc ccgaatcgaa atcccgcgct tggtgacgcc attggcgatc
     9841 tccatgggcg agaaagcgtg cacatgcatc gatggcaccc gcgccttgac cgcacgcact
     9901 aaatcggcgt agcccgtcac cggcaactcc ggatcgatgc cgccctgcat gcacacctcg
     9961 gtggcgccgg cgacgtgcgc ctcccacgcc cggtcggcca cctccgcggt cgacagcgaa
    10021 tacgcatcgg cgtcgccctt gcgttgggcg aacgcgcaga accggcagcc cgtgtagcag
    10081 atgttggtga agttgatgtt gcggttgacg acgaacgtca cgtcggcgcc gaccacgtcc
    10141 ttgcgcagtg aatcagccag tgccgcaacg gcatccaatg ccgggccgtc cgcggtggcc
    10201 agcgccagat actcgtcgtc gctgcagccc gcaggatcgc gctcggccga gcgcagggcg
    10261 gcgagcacgt cggtgtcgat gcgctcgggc gcccgcgccg cgagttcgtg caccttctcg
    10321 cgaatcgact cccagtcgcc gaacgcgctg tccaaatcgc tgcgggtctc ggtaatacga
    10381 ccctcggaat cgatcgccgt cgccaaatcg atccggccca gtgactcggc cgcctcgtcg
    10441 ggctcctgcc acggccgccc gaccggattc acgtcggcgg ccaggccggt gtccggatct
    10501 gccaacgcgt cgacgtgccc gcgcacccgc gggtcgatcc acgccgcacc cgcctgcacg
    10561 tacttcggct gagcggtc
//
LOCUS       DASRJA010000492        10958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_571094,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000492 DASRJA010000000
VERSION     DASRJA010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10958
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571094"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1284)
                     /gene="tet(V)"
                     /locus_tag="VFC01_21725"
     CDS             complement(28..1284)
                     /gene="tet(V)"
                     /locus_tag="VFC01_21725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(V)"
                     /protein_id="HZN82280.1"
                     /translation="MVDAGGWRVLAPFKFREYRLLMTAVSLSIFADGMFAVVLALQVI
                     ELSNDPVSLSVVTTCFGAGLVAFVLVGGLAADRINQRAIIIAVETVNVIAVSTIAVLG
                     LVGVLRIWHLAVAAAALGIAAAFFFPAYSAILPRILPAEQLLAANGVEGVVRPVFQRA
                     VGPAIAGLVVGMTFPSLGAVVVAALFAIGLLLLVATKPAMKPHVHQPDQERPHLLRDL
                     REGFVFMVRTPWLLWTLLFASMFVLVVLGPIEVLLPFIAKQRFEDGARSYGFILAFFG
                     VGSALGALAVSSGRLPRRYLTVMMTMWSVGSIPLVVVGVTSSFPLMAVATFVIGFTDG
                     AGMVIWGTLLQRRVPTEMLGRVSSLDFFVSLAFMPVSFAIVGPLSKVVSMQTIFLVAG
                     VAPVLLAAIAVYAARMRRDELAHPLR"
     gene            complement(1414..2235)
                     /locus_tag="VFC01_21730"
     CDS             complement(1414..2235)
                     /locus_tag="VFC01_21730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZN82281.1"
                     /translation="MTTTDSRSDAYTSIDDLSVKLADGVLSVTLNRPDSLNSLTAAML
                     KGVADALEQAAGDPRVKVVRLGGAGRGFSSGAGISEEDQANPGAAGTPADVLDAANRA
                     VRSIVNLPQPAVAVVQGPCAGVGASLALACDVVLASEKAFFTLAFTKIGLMPDGGASA
                     LIAASVGRHRAMRMALQAERIPAAEAFEWGLVTAVYPVDQFDAEVSKVVATLVSGPAV
                     ALRKTKHAINAATLTEFEGALEREKQGQLALIDSHDFREGTRAFQQRRSPNFTDS"
     gene            2311..3405
                     /locus_tag="VFC01_21735"
     CDS             2311..3405
                     /locus_tag="VFC01_21735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZN82282.1"
                     /translation="MAGPLHGLRVVELAGIGPGPHAAMLLGDLGADVVRVERPGKGPG
                     PATEPGRDYLLRNRRSVTANLKSDEGRDLVLRLVAKADVLIEGFRPGVTERLGLGPLD
                     CAEVNERLIYARMTGWGQTGPRSQQAGHDINYISLNGVLHAIGRAGERPVPPLNLVGD
                     FGGGSMFLVVGILSALWERERSGHGQIIDAAMVDGSSALSTMIWAFRAMGMWSDEPGV
                     NMLDTGAPYYDTYTCADGRYVSVGAIEPQFFAELLYKLGLDAADLPGQNDVSRWPELR
                     ARLTEVFASQDRDHWAKVFADSDACVAPVLSFAEVDTEPHNTERDTFYRENEYLFPAP
                     APRFSRTVPAKPTPPGVPGADTEAVLRDWV"
     gene            3423..4175
                     /locus_tag="VFC01_21740"
     CDS             3423..4175
                     /locus_tag="VFC01_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HZN82283.1"
                     /translation="MEIKDAVAVVTGGASGLGLATTKRLLDAGAQVVVLDIRGKEAVD
                     ELGERAVFAETDVTNEAAVTAALDAAEKLGPIRIVINCAGTGNAIKTLSKDGPFPLDL
                     FKKVIEINLIGTFNVIRLGAERIAKTEPINGERGVIVNTASVAAFDGQVGQAAYSASK
                     GGVVGMTLPIARDLSRELIRVVTIAPGLFDTPLLAGLPEPAKESLGKQVPHPSRLGKP
                     DEYGALAVHIVENPMLNGEVIRLDGAIRMAPR"
     gene            4172..5170
                     /locus_tag="VFC01_21745"
     CDS             4172..5170
                     /locus_tag="VFC01_21745"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HZN82284.1"
                     /translation="MTITTKFTETFGVEHPIVQGGMQWVGRAELVAAVANSGALGFIT
                     ALTQPTPADLAKEIAKCRDLTDQPFGVNLTILPTINPPPYDEYRQVIVDAGIKIVETA
                     GSNPAPHLPMFHDNGIKVLHKCTSVRHAVKAQSLGVDGISIDGFECAGHPGEDDIPGL
                     VLIPAAAAKIDIPMIASGGFADARGLVAALALGADGINMGSRFMCTVESAIHQNVKEA
                     IVAGSELDTELIFRPLRNTARVASNAVSREVVEILNRGGEFPDVKDLVAGVRGVKVFE
                     NGDLDAGIWSVGTAMGLINDIPTVGDLVARIVEEAEELIMGRLADMVELEAEENVA"
     gene            complement(5247..5378)
                     /locus_tag="VFC01_21750"
     CDS             complement(5247..5378)
                     /locus_tag="VFC01_21750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82285.1"
                     /translation="MWIDDTSADVIKVDFEALYHGDVLVEGDTSEQLDDEHTLPTAV"
     gene            complement(5468..6010)
                     /locus_tag="VFC01_21755"
     CDS             complement(5468..6010)
                     /locus_tag="VFC01_21755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZN82286.1"
                     /translation="MATTHVSTPTTTSLADLGLLILRIGVGATMIQAGLRKAFDFSTT
                     VGFMESGGWRLPKFAAFMVTAAETAGGIGLLLGLLTPLAACAVVAAMIDAWAVNVSAA
                     AFWSEPFNVPFVIAVGAAALLFTGAGAYSVDAKVFGSWRFGIRIAVGLLVVAIAAALL
                     TWILLNGTNPIHFTAPASAA"
     gene            6167..8443
                     /locus_tag="VFC01_21760"
     CDS             6167..8443
                     /locus_tag="VFC01_21760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN82287.1"
                     /translation="MLQRIALLAIAAPRRIIAVALLVMVACGIFGIPVAKHLSAGGFQ
                     DPTSESAQATKLLVDKFGQGDMELIISVKSDDGAQSPAARAVGTDIADQLKASPFVAQ
                     VNSAWTAPGPAAPALISKDGKTGLIVAGITGGESGAQKHAKELTERLVHDRDGVTVRA
                     GGEAMTYVQINGQSEKDLLTMESIAIPLSFVVLVWVFGGLISAALPLAVGGFAILGSM
                     AVLRAITLVTDVSIFALNLTVAMGLALAIDYTLLIISRYRDELADGADRDRALVRTMA
                     TAGRTVLFSAMTVALSMVAMVLFPMYFLKSFAYAGIAVVVFAAVAAVVVAPAAIALLG
                     DRLDSLDARRLARRIFSRPDPVRKPIEQNFWYRSTKFVMRRAVPIGVAIVALLLVLGA
                     PFLGIKWGFPDDRVLPQSLSAREVGDDLRTNFAVDSARNVTVVIPDTNGVTPADLDRY
                     ASALSQVPDVSSVSAPGGTFVGGRNAGSPSAATGFKDGSAFLTVGSTAPLFSDASESQ
                     LDRLRAVPPPTGQHVQLTGVAQINRDSSEAITSRLPMVLGIIAVITFVLLFLLTGSVV
                     LPLKALVLNVLSLTAAFGALVWIFQEGHLGALGTTSTGTLVANMPVLLFCIAFGLSMD
                     YEVFLVSRIREYWLKSGKTRADNDESVALGLARTGRVVTAAALLMSISFAALIAAQVA
                     FMRMFGVGLTLAVLADATLVRMLLVPAFMHVLGRWNWWAPKPLARLHERIGISESGDD
                     DAPTEKRERVAASVADTD"
     gene            8445..9140
                     /locus_tag="VFC01_21765"
     CDS             8445..9140
                     /locus_tag="VFC01_21765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN82288.1"
                     /translation="MAIQPLRRRRAPRGSGEQLRDEILDATTELLLETGHAKAVSIRS
                     VAQRVGVTPPSIYLHFADKDALLDAVCARYFEKLDEEMQRVAADQPTTIDVLRAQGLA
                     YVRFALKTPELYRIAMMGESREGSDVDVALTTSAFVHMRKSVESLMEEGIYPPGDPVA
                     KALELWTAGHGVAALLISRPYLPWGDAEEFADRVLRSVCVGHIVSGAIDPDLPPTEAV
                     ALLKGMFDEQHRR"
     gene            9121..9691
                     /locus_tag="VFC01_21770"
                     /pseudo
     CDS             9121..9691
                     /locus_tag="VFC01_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(9747..10460)
                     /locus_tag="VFC01_21775"
     CDS             complement(9747..10460)
                     /locus_tag="VFC01_21775"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HZN82289.1"
                     /translation="MQVTVLSGAGISAESGVPTFRDAETGLWAKVDPYEISSTEGWHD
                     HPDRVWAWYLWRHHMMGSVQPNNGHLAVAAWQDYADVHVVTQNVDNLHERAGSKQVYH
                     LHGSLFEFRCDRCQSEYRDELPAMPEPVASVDPPRHACGGLIRPNVVWFGEALPDEAW
                     QRSVDAVTAADLVVVVGTSSIVYPAAGLPEFAVANGIPVIEVNPERTPLSDAATAVVR
                     ESAAAALPGLLQRLPALLG"
     gene            10498..10863
                     /locus_tag="VFC01_21780"
     CDS             10498..10863
                     /locus_tag="VFC01_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN82290.1"
                     /translation="MAELGDWVRIDPNAAKPLFDQLRTQIIDGIRDGKLPPGTRLPTV
                     RELAAQMSLAVNTVARAYRELESAGILETRGRFGTFVARVDPADTAMATAAHTFVSAA
                     RALGIDKTEAMRYVDAAFG"
BASE COUNT         1932 a         3596 c         3669 g         1761 t
ORIGIN      
        1 gctcgcactg ccaccggtgt tcggcgtcta gcgcaacggg tgagccaact cgtcccggcg
       61 catccgggcc gcgtacaccg cgatcgcggc gagcagcacc ggcgcgacgc cggccaccag
      121 gaaaatcgtt tgcatcgaaa cgactttcga cagcgggccg acgatcgcga acgacaccgg
      181 catgaaggcg agcgagacga agaagtccag gctcgatact cggcccagca tctccgtcgg
      241 cacgcgccgc tgcagcagcg tcccccagat caccatgccc gcgccgtcgg tgaagccgat
      301 gacgaacgtc gcgacggcca tcaacgggaa ggacgacgtg acgccgacga ccaccagagg
      361 tatggagccc acgctccaca tcgtcatcat caccgtgaga tagcgacgcg gcagccgtcc
      421 cgacgacacc gcgagcgcgc cgagtgcgct gccgacgccg aagaacgcga ggatgaaacc
      481 gtacgagcgt gcgccatcct cgaagcgctg cttggcgatg aagggcagca gcacctcgat
      541 cggcccgagc acgacgagca caaacatgct cgcaaacagc agcgtccaca acagccacgg
      601 tgtgcgcacc atgaacacga agccctcgcg cagatcgcgc agcaggtgcg gccgctcctg
      661 atccggttgg tgcacatgcg gtttcatcgc gggtttggtg gcgaccagca gtagcaggcc
      721 gatggcgaac aacgcagcga ccaccaccgc gcccagagag gggaacgtca tgccgaccac
      781 caggccggcg atggccgggc cgacggcgcg ctggaacacc ggacgcacga cgccttcgac
      841 gccgtttgcg gcgagcagct gctcggccgg aagaatccgg ggcaggatcg cgctgtaggc
      901 ggggaagaag aacgccgcgg cgatgccgag cgcggcggca gccaccgcga gatgccagat
      961 ccgcaggacg ccaacgagtc ccaacaccgc gatcgttgac accgcgatca cgttgaccgt
     1021 ctcgacggcg atgatgatgg cacgctgatt gatgcggtcg gcggccagcc cgccaaccaa
     1081 cacgaacgca accaggccgg cgccgaagca ggtggtgacc accgacagcg atacgggatc
     1141 gttggacagt tcgatgacct gaagggccaa cacaaccgcg aacatgccgt cggcgaagat
     1201 cgacagcgac accgcggtca tcagcaggcg gtactcgcgg aacttgaagg gtgcgagcac
     1261 gcgccagccg ccggcgtcga ccaccggctg ctcaatgtcg aattgcgtac tcaccactag
     1321 atggtcgcgg agcgacgggt ctacagtcca ccgaatttct ggcgacttgt gcacgaaaat
     1381 ccgcgctggg cgcggaaaaa cgtgcacaag tcgctaggag tcggtgaagt tcggggaccg
     1441 gcgctgctga aacgcccggg tcccctcgcg gaagtcgtgc gagtcgatca gcgccagctg
     1501 accctgcttc tcacgctcca gcgcaccctc gaattcagtg agcgtcgcgg cgttgatcgc
     1561 atgcttggtc ttgcgtagcg ccaccgcagg cccggacacc agcgtcgcca ccaccttcga
     1621 gacctcggcg tcgaactgat cgactggata caccgccgtc accaggcccc actcgaaagc
     1681 ctccgccgcc ggaatccgct cggcctgcag cgccatccgc atggcgcggt ggcggcccac
     1741 cgatgcggcg atcaacgccg acgcgccgcc gtccggcatc agcccgatct tcgtgaacgc
     1801 cagcgtgaaa aaagccttct ccgaagctaa tacgacgtcg cacgccagcg ccaatgacgc
     1861 tccgaccccg gcgcacggtc cctgcaccac cgcgaccgcc ggctgcggca agttcacgat
     1921 cgaccgcacc gcgcggttgg ccgcgtctag cacatcggcg ggcgttccgg cagcaccggg
     1981 attggcctgg tcctcttcgc tgatacccgc gcccgacgag aaaccccgcc ccgccccgcc
     2041 gaggcgcacc accttgaccc gcggatcgcc cgccgcttgc tccagcgcgt cggcgacccc
     2101 cttcagcatc gccgctgtca gcgagttgag gctgtctggc cgattcagcg tcaccgacag
     2161 cacaccgtcg gccagcttga cggacaggtc gtcgatgctg gtgtaggcgt cgctgcggga
     2221 gtccgttgtc gtcatagaag gcaccctagg tctggcgaca tggttataca agtaagctat
     2281 gtcggcggtc acctacggag ggcggtagac atggctggac cactgcacgg actgcgcgtc
     2341 gtcgaactgg ccggcattgg cccgggtccc cacgcggcga tgctgctcgg cgacctcggc
     2401 gccgatgtcg tccgcgtcga gcgtcccggc aagggccccg ggccagctac cgaacccggc
     2461 cgcgactacc tgttgcgcaa ccgccgctcc gtgaccgcga acctcaagag tgacgagggc
     2521 agagacctcg tgctgcgtct ggtcgccaag gccgacgtgc tcatcgaggg cttccgaccc
     2581 ggcgtcaccg agcgcctcgg cctcggccca ctggactgcg ccgaggtcaa cgagcggctg
     2641 atctatgccc gcatgaccgg ctggggccag accggcccgc gcagccagca ggccggccat
     2701 gacatcaact acatctcgct caacggtgtg ctgcacgcga tcggccgcgc aggcgaacgt
     2761 cccgtcccgc cactgaacct cgtcggcgac ttcggcggtg gctcaatgtt tctcgtcgtc
     2821 ggcatcctgt cggcgctatg ggaacgcgaa cgctcaggcc acggccagat catcgacgcg
     2881 gccatggtcg acggctccag cgcactctcg acgatgatct gggcgttccg cgcgatgggc
     2941 atgtggagcg acgagcccgg cgtcaacatg ctcgacaccg gcgcgcccta ctacgacacc
     3001 tacacctgcg ccgacggccg ctacgtctcg gtcggtgcca tcgagccgca gttcttcgcc
     3061 gaacttctct acaaactcgg actcgacgcg gccgacctgc ccggccagaa cgacgtcagc
     3121 cgctggcccg agctgcgcgc ccggctcacc gaggtcttcg cgtcgcagga tcgcgaccat
     3181 tgggccaagg tgttcgccga cagcgacgcg tgcgtggcac cggtgctgtc gttcgccgag
     3241 gtcgacaccg agccgcacaa caccgagcgc gacaccttct accgcgagaa cgaatacctg
     3301 tttcccgcac cggcgccgcg gttttcgcgc acggtgcccg ccaagccgac gccgccgggg
     3361 gtgcccggcg ccgacaccga agccgtccta cgcgactggg tatagagaaa ggaacgcaat
     3421 cagtggagat caaagacgcc gtagccgtcg tcacgggtgg tgcatccggg ctgggtctgg
     3481 cgacaacgaa gcgactgctg gacgcgggcg ctcaggtcgt cgtcctcgac atccgcggca
     3541 aggaggccgt cgacgaactc ggtgagcgcg ccgtcttcgc cgagaccgac gtcaccaacg
     3601 aagccgccgt gaccgccgcg ctggacgccg ccgaaaagct cggcccgatc cgcatcgtga
     3661 tcaactgcgc gggcaccggc aacgcgatca aaacgctgag caaggacggg ccgttcccgc
     3721 tcgacctgtt caagaaggtc atcgagatca atctgatcgg caccttcaat gtgatccgcc
     3781 tgggcgccga gcgcatagcc aagaccgagc cgatcaacgg cgagcgcggt gtcatcgtca
     3841 acaccgcatc ggtcgccgcc ttcgacggac aggtcggtca ggccgcctac tcggcgtcca
     3901 agggcggggt ggttggcatg acgctgccga tcgcgcgcga cctgtcgcgt gagctgatcc
     3961 gcgtcgtcac gatcgcgccc ggcctgttcg acacgccgct gctggcaggc ctgcccgagc
     4021 cggccaagga gtcgctgggc aagcaggtgc cgcatccgtc gcggctgggc aagccggacg
     4081 agtatggcgc gctggccgtg cacatcgtgg agaacccgat gctgaacggc gaagtgatcc
     4141 gcctcgacgg cgcgattcgg atggcgccgc gatgacgatc accacgaagt tcaccgagac
     4201 gttcggcgtc gagcacccga tcgtgcaggg cggcatgcag tgggtcggcc gcgcggaact
     4261 cgttgcggcc gtggccaact cgggcgcgct cggcttcatc accgcgctca cccagccgac
     4321 gcctgcggac ctggccaagg agatcgccaa gtgccgcgac ctgacggacc agccgttcgg
     4381 cgtcaacctg acgatcctgc cgacgatcaa cccgccgccc tatgacgagt accggcaggt
     4441 gatcgtcgac gccggcatca agatcgtcga gacggccggc tcgaaccccg caccgcacct
     4501 gccgatgttc cacgacaacg ggatcaaggt gctgcacaag tgcacctcgg tccgccatgc
     4561 cgtcaaggcg cagagtctgg gcgtcgacgg catcagcatc gacgggttcg aatgcgccgg
     4621 gcatcccggc gaggacgaca tccccggtct ggtgctgatc ccggccgcgg cggccaagat
     4681 cgacatcccg atgatcgcct cgggtgggtt cgccgacgcg cgtggcctgg tggccgcgct
     4741 ggccctgggg gccgacggca tcaacatggg ctcacggttc atgtgcaccg tcgagtcggc
     4801 cattcaccag aacgtcaagg aagccatcgt cgcgggcagc gagctcgaca ccgagctgat
     4861 cttccgtccg ctgcgcaata cggcgcgggt ggcaagcaac gcggtgtcgc gtgaggtggt
     4921 cgagatcctc aaccgcggcg gcgagttccc cgacgtgaag gacctggtcg caggcgtgcg
     4981 cggagtcaag gtgttcgaga acggcgacct ggacgcgggc atctggtcgg tcggcaccgc
     5041 gatgggtctg atcaacgaca tcccgacggt cggcgacctg gtggcgcgca ttgtcgagga
     5101 ggccgaggag ctgatcatgg gccggttggc cgacatggtt gagctcgaag cagaagagaa
     5161 cgtggcgtag aaaaaggaat atcgacgaag ggcccgtact gggcaaactg tcatgggtga
     5221 acgcgcgccg cgaggcgcgc gcttactcag accgcggtgg gcagagtgtg ctcgtcgtcg
     5281 agctgctccg aggtgtcgcc ctcaaccagg acatcgccgt ggtagagcgc ctcgaagtca
     5341 accttgatga catcagcact ggtgtcgtcg atccacatgt actgaacagt aaccatcacc
     5401 attaagagat ctttgagtca cggttcggaa aacggtggca attcttaccg ccgggtaggt
     5461 tcgtccatca agcggccgag gccggagcgg tgaagtggat cgggttcgtc ccgttcagca
     5521 atatccaggt cagcagcgcc gcggcgatcg ccacgacgag caggccaacc gcgatccgga
     5581 tcccaaagcg ccagcttccg aacaccttgg cgtcgacgga gtaggcgcct gcgccggtga
     5641 acagcagcgc ggcggccccg actgcgatca cgaacgggac gttgaacggt tctgaccaga
     5701 acgcggctgc ggagacattg accgcccacg cgtcgatcat cgcggcgacg acagcacatg
     5761 ccgccaacgg cgtcagcaaa cccaatagca gaccgatgcc acccgcggtt tcggccgcgg
     5821 tcaccatgaa tgcggcgaac ttcggcagcc gccagccacc ggattccatg aagccgacgg
     5881 tcgtgctgaa gtcgaaggct ttccgcaggc cggcctgaat catcgtcgcg ccgactccga
     5941 tgcgcaggat cagcaggccg agatcggcta gggacgtcgt ggtgggggta ctcacatgag
     6001 tggtcgccat gaccggcagc ttatgcggct gtgagaacgc tgtgaatgct tgccttgtga
     6061 acaagcggcg gccgcgtgac gtagttacgg gtggaagaga gtttgttgac tcccttaact
     6121 tatcgcggat aacttagcga cggtatccaa cttggaagga cgatacgtgc tgcaacgaat
     6181 cgcactgttg gccattgccg cgccgcgacg aatcattgcg gtcgcgctcc ttgtgatggt
     6241 ggcctgcggc atcttcggca tacccgtcgc caagcacctg tcggccggcg gctttcagga
     6301 tccgacgtcg gagtctgcgc aagcgacgaa gctgctcgtc gacaagttcg gccagggcga
     6361 catggagctg atcatcagcg tgaagtcgga tgacggcgcc cagagtccgg ccgcccgcgc
     6421 cgtcgggaca gatatcgccg accagctgaa ggcgtcgccg ttcgtggcgc aggtgaactc
     6481 ggcatggacc gcgccgggac ccgccgcgcc agcattgatc agtaaggacg gtaagaccgg
     6541 gctgatcgtc gcgggcatca ccggcggtga gagcggcgcc cagaagcatg ccaaggagct
     6601 gaccgagcga ctggtccacg accgtgacgg cgtcaccgtg cgcgcgggcg gcgaggcgat
     6661 gacctacgtg cagatcaacg gtcaaagcga aaaagacttg ctgacaatgg aatccatcgc
     6721 gatcccgctc agcttcgtgg tcctggtatg ggtgttcggc ggcctgattt ccgccgcgct
     6781 gccattggcc gtcggtggct tcgcgattct gggttcgatg gcggtgctgc gggccatcac
     6841 gctggtcacc gacgtgtcga tctttgcgct gaacctgacc gtcgcgatgg gcctggccct
     6901 ggccatcgac tacacgctgc tgatcatcag ccgataccgc gacgagctcg ccgacggcgc
     6961 cgaccgcgac cgcgcgctgg tacgcaccat ggcgacagcg gggcggacgg tgctgttctc
     7021 ggcgatgacg gtcgcgctgt cgatggtcgc gatggtgctg ttcccgatgt acttcctgaa
     7081 gtcgttcgcc tatgcgggta tcgcggtggt ggtgttcgcg gccgtggccg cggtcgtggt
     7141 ggcgccggcc gcgatcgcgc tgctcggcga ccggctggat tcgctggatg cccggcggct
     7201 ggctcgtcgg attttcagca ggcccgatcc ggtgcgcaag ccgatcgagc agaacttctg
     7261 gtaccggtcc acgaaattcg ttatgcggcg ggcggttccg atcggggtgg ctatcgtcgc
     7321 gctgctgctg gtgctcggcg ctccgttcct gggcatcaaa tggggcttcc ccgacgaccg
     7381 cgtgctgccg cagtcactgt cggcccgcga ggtcggtgac gacctgcgca ccaacttcgc
     7441 cgtcgactcc gcccgcaacg tcaccgttgt cattccggac accaacggcg tcacacccgc
     7501 cgacttggac cgctacgcgt cggcgctgtc gcaggtacct gacgtgtcgt cagtgtcggc
     7561 gccgggcggc acgttcgtcg gcggtaggaa cgccggatcg ccgtcggccg ctaccggatt
     7621 caaggacggc agcgcgttcc tcacggtcgg aagcaccgcg ccgctgttca gcgacgcgtc
     7681 tgagtcgcag ctcgaccggc tacgggcggt gccgccgccg acgggccagc acgtgcagct
     7741 gaccggcgtc gcgcagatca accgcgacag ctccgaggcc atcacgtccc ggctgccgat
     7801 ggtcctcggc atcatcgccg tgatcacctt cgtgctgctg ttcctgctca ccggcagcgt
     7861 ggtgttgccg ctgaaggcgt tggtgctcaa cgttttatcg ttgacggccg cgttcggcgc
     7921 gctggtgtgg atcttccagg agggccacct cggcgcgctc ggcacgacat cgacgggaac
     7981 gctggtcgcc aacatgccgg tgctgctgtt ctgcatcgcg ttcggcctgt cgatggacta
     8041 cgaggtgttc ctggtgtcgc ggatccggga gtactggctg aaatccggga agacgcgcgc
     8101 cgacaacgac gaaagcgtcg cgctcggcct ggctcgcacc ggccgcgtcg tcaccgctgc
     8161 cgcgctgttg atgtcgattt cgttcgcggc actgatcgcc gcacaggtgg cgttcatgcg
     8221 gatgttcggt gtcggcctga cgctggcggt gctcgccgac gcgaccttgg tccgcatgct
     8281 gctggtgccg gcgttcatgc atgtgctcgg ccggtggaat tggtgggcgc cgaagccgct
     8341 cgccaggttg cacgagcgga tcgggatcag cgagtccggg gacgacgacg cgccaacgga
     8401 gaaacgcgaa cgagtcgcgg cctcggtggc ggacactgat tagcgtggcg atccagcccc
     8461 tgcgacgacg ccgcgccccg cgcggttccg gcgaacagtt gcgtgacgag atcctcgacg
     8521 caacgaccga actgctcttg gaaaccgggc acgcgaaggc ggtgtccatc cgatcggtcg
     8581 ctcagcgggt cggtgtcacc ccgccatcga tctatctgca cttcgccgac aaagatgcgc
     8641 tgctggacgc cgtgtgtgcc aggtacttcg agaagctcga cgaggagatg cagcgggtcg
     8701 ccgccgatca accgacgacc atcgatgtgc tgcgcgcgca gggccttgcc tacgtccgct
     8761 tcgcgttgaa aacccctgag ctgtaccgga ttgcgatgat gggtgaaagt cgcgagggca
     8821 gtgatgtgga cgtggcgctg acaacgtcgg cgttcgtgca catgcgtaag tcggtggaat
     8881 cgctgatgga ggagggtatc tacccgccgg gtgatcccgt cgccaaggcg ctggagttgt
     8941 ggacggcggg ccatggcgtg gcggcgctgc tgatctcgcg accgtatctg ccgtggggtg
     9001 acgcagagga attcgcggat cgggtgctga gatcggtgtg tgtcggccac atcgtgtccg
     9061 gtgcgatcga tcccgatctg ccgccaaccg aggccgttgc attgctgaag ggaatgttcg
     9121 atgagcagca ccgtcgataa tccgttcttc gcgcggatgt ggaccgtgat ggcgcgccac
     9181 gaaacccgag tcggtgaagc ggctgcgtcg cgagaacctg gctggcctgt cgggtcgggt
     9241 gctggaagtc ggcgccggca cggggacgaa tttcgagctg taccccaaca ccgtgaccga
     9301 ggtcgtcgcc gtcaccgtca gcaccgacac ggtggaacag ttttcagacg gcgagccgtt
     9361 tgacgcggcg gtgtgctcgc tggtgttgtg ctcggtcgac gagcccgatc atgttgtgcg
     9421 tcatgtgctt tcactgctga agccgggcgg cgaactgcgc tatctcgagc atgtcgccag
     9481 caccggtgcg cgggagcggc tgcagaagct cgccgacgcg acgctgtggc cgcggatggc
     9541 gggcaactgt cacacgcatc ggcacaccga gcagacgatc gtcgacgcgg gtttcacggt
     9601 gtccggtgca cgccgcgaaa gggtgttgcc ggcctgggtg cccgtgccga cctcggaggt
     9661 cacgatcggc cgcgcggtca aaccggcctg attcgcgatt tgtgcacggt ttcacgcgct
     9721 gagcaccaat tttcgtgcac gaatcgctag ccgagcaggg ccggcagcct ctgcagtagg
     9781 ccgggcaggg cggcggccgc agattcgcgg acgacggcgg tggccgcgtc agagagcggc
     9841 gtgcgctcgg ggttcacctc gatgacaggg ataccgtttg ccaccgcgaa ctcgggcagt
     9901 ccggcggccg ggtacacgat tgatgaggtg cccaccacga ccacgaggtc ggccgcggtc
     9961 accgcatcga ctgaccgctg ccacgcctca tcgggcagcg cttcgccgaa ccacaccacg
    10021 ttgggccgga tcaatccgcc gcacgcatgc cgcggcgggt cgaccgacgc gacgggttca
    10081 ggcatcgcgg gaagctcgtc gcggtactcg ctttggcaac ggtcgcaacg gaattcgaac
    10141 aagctgccgt gcaggtggta gacctgctta ctgcccgcgc gctcatgcag attgtcgacg
    10201 ttctgggtga cgacgtgcac atcggcgtag tcctgccacg ccgcaaccgc gaggtggccg
    10261 ttgttgggct gaaccgagcc catcatgtgg tgccgccaca gataccacgc ccacaccctg
    10321 tccggatggt cgtgccaacc ctccgtactg gagatttcgt acgggtcgac cttcgcccac
    10381 aggcccgttt cggcgtcgcg gaatgtcggc acgccgctct cggcggaaat gccagctccg
    10441 ctgagcaccg tcacctgcac accaccaaag tagcccgttt aagcgatact gatgcacgtg
    10501 gccgagttgg gggattgggt tcgtatcgat ccgaacgccg ccaagccgct ttttgaccag
    10561 ctcaggacgc agatcatcga cgggatccgg gacggcaagc tgccgccagg cacccggttg
    10621 ccgacggtcc gcgaactggc ggcgcagatg agcctggcgg tcaatacggt ggcgcgcgca
    10681 tatcgggagt tggagtcggc gggcatcctc gaaacccggg ggcgtttcgg cactttcgtc
    10741 gcgcgagtcg atccggccga caccgcgatg gcgacggcgg cgcatacgtt tgtgtcggcg
    10801 gcgcgtgcgc tggggatcga caagaccgag gcgatgcggt acgtcgacgc ggcgttcggc
    10861 tgatcagccg cgccagcgca gcacgtcaag agcgaccgcg acctcgacgc tggaatcggc
    10921 gagcggccgg ggtaacagtt cgtccgcgcg ggcgagcc
//
LOCUS       DASRJA010000493         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1980089,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000493 DASRJA010000000
VERSION     DASRJA010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980089"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VFC01_21785"
     CDS             complement(<1..624)
                     /locus_tag="VFC01_21785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HZN82291.1"
                     /translation="MTTTKTIIREHETDLVVREARTVAADVVVLSLADPQGAELPPWT
                     PGAHIDLILGDDLTRQYSLCSSPSKPDVWRVGVLRAPDSRGGSEHVHAALSPGSTVRV
                     RGPRNHFPLVASSRYLFIAGGIGITPILPMIAEAEAAAADWRLFYGGRERASMAFVDE
                     LASYGDRVTIVPQDEMGMLDLESVLGTPHPDTLVYCCGPEGLLSAVEK"
     gene            806..1528
                     /locus_tag="VFC01_21790"
     CDS             806..1528
                     /locus_tag="VFC01_21790"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZN82292.1"
                     /translation="MRALMIQHDHICTPGFVGERFTDRGYELVVHQVVPENHFQSPNV
                     ETQFPEPTNFDLIISMGAPWSVYDHENIGSWVLPELAMLHNAHVDHVPVFGICFGGQL
                     LAAAHGGSVSRSRAPEIGWVEIESDDESLVPAGEWFQWHYDSWELPEPATELARNASS
                     SQAFVLGRNLAVQFHPEMSSAILASWIATGGAAEMNRLGMNVDELVRQTAERDASNRE
                     RAHKLVDLFLDHVAVGSEAGLG"
     gene            1525..2484
                     /locus_tag="VFC01_21795"
     CDS             1525..2484
                     /locus_tag="VFC01_21795"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HZN82293.1"
                     /translation="MIHDHGHTFRQAAGRFASGVTVVTTRVSKGAYGVTVSSFASLSL
                     NPLLVTVSINRSSPMLEYVRSAEAFGISVLASDQQQVADYFATRGRTPEPAGFATVPT
                     TARQTSAPIIDGCLSWFDCTVEDVLPGGDHEILVGRVVAAGGGTGEPLVYWSGGYRAL
                     TTEVAESDRLANASDGLAVAYHLLDVGPEEMLDAQSSVEPAMAALAASRTSAEEWDRL
                     KELVDQSELVIDQPDEFNQLALAFHTAIAEASRNRVLQATLASLGQVQSIHYRDRGSP
                     ESARAAVEGHRRLLAVLRQGDAEAARAEMQSHLVAVRDQLQIG"
     gene            complement(2508..>3275)
                     /locus_tag="VFC01_21800"
     CDS             complement(2508..>3275)
                     /locus_tag="VFC01_21800"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HZN82294.1"
                     /translation="GAGAALPPLLLDDEEAVAVAVGLRTAAGGTITGIEEASVRALAK
                     LEQVFPSRLRHRVSALHSVTMPLPGAGPPVDPAILTVVATAARDRQRIRFGYTSHDGT
                     RGSRAAEPHRLVHTDSRWYLLAWDPARDGWRTFRADRISTPQITGPRFTPRDPPGGDA
                     AAYVSRSVSSAPYRYQARIRLHAPAKVAAERLPPTVGHLQADGEHACILHTGAESPDV
                     LAIYVAVIGTEFEILDPPELAEHILALAGRLTRAVRH"
BASE COUNT          552 a          996 c         1131 g          596 t
ORIGIN      
        1 tttctcgact gcgctgagga ggccttcggg accgcagcaa tagaccagcg tgtctgggtg
       61 cggggttcca aggactgatt ccagatcaag catgcccatc tcgtcctgcg gcacgatcgt
      121 cacccggtcg ccgtacgagg ccagttcgtc gacgaaggcc attgacgccc gttcgcggcc
      181 gccgtagaac aaccgccagt cggcggccgc cgcctcagcc tccgcgatca tgggcaagat
      241 cggcgtaatg ccaataccgc cggcgatgaa tagatagcgg gagctggcga ccagtggaaa
      301 gtggttccga ggtccgcgaa cccgcaccgt tgaaccggga ctgagggcgg catgcacgtg
      361 ttccgagccg ccgcggctgt cgggcgcgcg cagcacgccc acgcgccaga catcgggttt
      421 tgacgggctg ctgcacagtg agtactggcg agtcaggtca tcgcccagaa tcagatcgat
      481 gtgagcaccg ggcgtccacg gcgggagctc ggcgccctgc gggtcggcga ggctgagcac
      541 gacaacgtcg gctgcgacag tccgcgcttc tcgcaccaca aggtcggtct cgtgctcccg
      601 gatgatcgtc ttcgtggtcg tcactgtcac aatcgcgtcc gcctgtcgca gcagcccaac
      661 atttgtttgg atacgaacga tagcggcggg aggggcgggc gacaagatgt agccgagccc
      721 gagacctgct gatccctggg cttgtcgcca atgcgccctg agcttgtcga agggcactcg
      781 ttcggatcca aattagactc gggccgtgcg cgccctgatg atccagcatg accacatctg
      841 cacaccgggc ttcgtcggcg aacgctttac cgaccgcggc tacgaactgg tcgtacacca
      901 ggtggtgcct gaaaaccact ttcaatcacc caatgtcgag acccagttcc ctgagcctac
      961 gaacttcgac ctgatcatct ccatgggcgc cccgtggtct gtatatgatc atgaaaacat
     1021 cgggtcctgg gtgttgcccg agctcgccat gctgcataat gcccatgttg atcatgtgcc
     1081 ggtgttcggg atctgtttcg gtggacagct gctcgccgca gcgcacggag ggtctgtgtc
     1141 acggtctagg gcaccagaga tcggctgggt cgagatcgag tccgacgatg agtccttggt
     1201 ccccgcgggc gagtggttcc aatggcatta cgacagttgg gagctgcccg aaccggcgac
     1261 cgaactcgcg cgtaatgcca gctcgtcgca ggcctttgtc ctcggccgga acctcgccgt
     1321 acagttccat cccgagatga gctccgcgat ccttgctagc tggatagcga ccggcggagc
     1381 cgcggagatg aaccggctgg gtatgaacgt tgacgaattg gtacgccaga cggctgagcg
     1441 ggatgcgtcc aaccgtgaaa gggcgcacaa actcgtcgac ctgtttctcg accatgtggc
     1501 ggtcggatca gaggccgggc tcgggtgatt cacgatcatg gccacacgtt tcggcaagct
     1561 gccggacggt tcgcctctgg cgtcactgtt gtcacgacgc gagtttccaa aggtgcctat
     1621 ggggtgacgg tgtcgtcctt cgcatccttg tcgttgaatc cgctcctggt cactgtgtcg
     1681 atcaaccgat caagcccgat gctcgagtac gtgcgttccg cagaggcatt tggtatcagt
     1741 gtgctcgcaa gtgatcaaca gcaggtggcc gactacttcg ccacacgagg gcgtacgccc
     1801 gagccggccg gttttgcgac ggtgccgacc acggctcggc agaccagtgc gccgatcatt
     1861 gacggctgcc tcagctggtt cgactgcact gtcgaggacg tccttccagg aggcgatcat
     1921 gagattctgg tcggccgcgt cgttgcggcg ggcggcggaa ctggtgagcc tctggtctat
     1981 tggtcgggtg gatatcgcgc attgacgacg gaggtggctg agtcagaccg gctggcaaac
     2041 gcctccgacg gcctcgcggt ggcctatcac ctactcgatg tcggaccgga agagatgctc
     2101 gacgcacaaa gctccgtcga accggccatg gctgcgctcg ctgcgtcgcg cacctcagcc
     2161 gaagagtggg accggctgaa ggagcttgtt gatcagtcag aactggtgat tgatcagcct
     2221 gatgaattca accagctggc gcttgctttc cacactgcga tcgccgaggc ctccaggaat
     2281 cgtgtgctgc aggccaccct cgccagcctc ggtcaggtgc agtccattca ctatcgtgac
     2341 cgtggcagtc cggaatcggc gcgggccgct gtcgaaggcc atcggcggtt gctggcggtg
     2401 ctgcgccagg gtgatgccga ggcggcacgt gcggagatgc agagccattt ggttgcggta
     2461 cgcgaccagc tgcagatcgg ctagagagct gtcgagatcc cggcgggtca atggcgtacc
     2521 gcgcgggtga gcctgccggc cagggcgagg atgtgctcgg ccagttcggg cggatcgagt
     2581 atctcgaact cggtgccgat gacagctacg tagatggcga gcacgtcagg tgattcggcg
     2641 ccggtgtgca ggatgcaggc gtgttcgccg tcggcttgga ggtggccgac ggtgggcggg
     2701 agacgctcgg cggctacctt ggccggggcg tgcagccgga tgcgggcctg gtagcggtag
     2761 ggcgcggacg acaccgagcg ggatacgtag gcggcggcgt cgccgccggg cgggtcacgc
     2821 ggggtgaatc gtggcccggt gatctgggga gtgctgatcc ggtcggcgcg gaaggtgcgc
     2881 cagccgtccc ttgccggatc ccaggcgagc agataccagc ggctgtcagt gtgcaccagg
     2941 cggtgcggtt cggcggcgcg gctgccccgg gtgccgtcgt ggctggtgta gccgaacctg
     3001 atccgctgcc ggtcgcgggc ggcggtggcg acgacagtca ggatcgccgg gtcgaccggc
     3061 ggtcccgcac cgggcagcgg catggtgacc gagtgcagcg cgctgacgcg gtggcgcagc
     3121 cgggagggaa acacctgttc cagcttggcc agcgcgcgga ccgaggcttc ctcgatcccg
     3181 gtgatagtgc ccccggccgc tgtgcggagg ccgaccgcga ccgcgacggc ttcctcgtca
     3241 tcaagcagca acgggggcag cgccgcgccc gcgcc
//
LOCUS       DASRJA010000494        12076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1319338,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000494 DASRJA010000000
VERSION     DASRJA010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12076
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319338"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VFC01_21805"
     CDS             complement(<1..1163)
                     /locus_tag="VFC01_21805"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN82295.1"
                     /translation="MATDERSREEPNSVVIVGSGFTGFECARHLARKLRKHNASADIS
                     IISPVDYMLYTPLLPDVAGGLVDARFVAIPLANALRDVQQIRGRVDGVDFDGRTVTFT
                     DPEERARTLPWDRLVLTPGSVTKLFDVPGLAEHARGLKSTAEALFLRDHVLEQLELAD
                     VDDDPGRAAARRTIVVVGASYSGTELAVQLRALADSAAKQMNFDPADVKFVLLDLAEQ
                     VMPEVGEKLGAAAQRVLQSRGIDVRLGITLKEVHSEHVVLSDDSLIRTHTVAWVTGVT
                     GAPLIEKLGLPTEKGRLKVETDLRVPGQPDVFSAGDAAAVPDVTQPGKVTPPTAQHAI
                     RQGKLLGRNVAASLGYGTMKTYKHRNMGLVVDLGPHYAVANPLNIQLSGYAAKA"
     gene            complement(1182..2039)
                     /locus_tag="VFC01_21810"
     CDS             complement(1182..2039)
                     /locus_tag="VFC01_21810"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HZN82296.1"
                     /translation="MTSAPVHLTGPAEDTRRAYRPTLNALRIAVARSADLWRNIDKPD
                     APAPGLQWTAAETAAHVVGDLRDYTQALTRHTGGYMTHANRPTESPSRLSAKVNARHL
                     EEIRERNLSRLADMLEVAADAYLNAAANADLSAEIATPNGLVIGPPTMAALLLGEQLI
                     HGLDISRTAKIRWPIAADDAFLVIPGVLTVAPQYLRPQRAAGVRISFELRMRGGASYR
                     LTVDDGTAVVTAAGEKADCHITADPVAFLLLGYGRIGQLSPVLRGQLRPGGRKPWLAM
                     RFGTLLETP"
     gene            2109..2708
                     /locus_tag="VFC01_21815"
     CDS             2109..2708
                     /locus_tag="VFC01_21815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82297.1"
                     /translation="MSDPPASLRAEQVAQTRAALVAAGRRLFGEKGFRATSVEDLARE
                     ARVTTGALYHHFPNKTALFEAVFLQAHTDLMTASTKAARGASDGIDELARGFEAFLDG
                     VLQPDMQRILIVDGPAVLGLARYTELDEQYAHAAIVHALTAAAQAGTITVEDPETATR
                     LLLGALTRGAMLIANAADPVQTRHAVAKSMRALLSSFTP"
     gene            2732..3544
                     /locus_tag="VFC01_21820"
     CDS             2732..3544
                     /locus_tag="VFC01_21820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82298.1"
                     /translation="MTRPRPLRRFDPFRPIDMLAALWSSAAVAPVSSGAAAAYRTLFM
                     TVRRLVVGRRLAVRLEDGDLTLTVTEFDSRLDVRALSVGQLNDVRLAARDICWHGSRF
                     DRATAVLHNVHMKPTAPPVLVAAPVELTLEIPAPVLDDLFRWAAPRLSGEVGADAIAR
                     LRLASRPLLGHIEVDASLDGSTLWLKPRGLAVRQRHWRLPARTPAYPVHLPELPQGLS
                     LTGISFAPGVVCLSGTLPEWRMDVPRKRLEDIINQLSVVGVPLNLTRLGRLF"
     gene            3662..4570
                     /locus_tag="VFC01_21825"
     CDS             3662..4570
                     /locus_tag="VFC01_21825"
                     /inference="COORDINATES: protein motif:HMM:NF038175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IniB N-terminal domain-containing protein"
                     /protein_id="HZN82299.1"
                     /translation="MINLLDYILDLFRDESHAQAFVANPDQAMADAGLSTVNSAQLQS
                     VAATAIPSLAMGGSGDAVTDLQQAVSSYYGFQPQPDVAPQTDFSPSTDLASNNSFMSP
                     TTNITDDHSVSFGMGDITLGDKTTAQGDGAVAVGGSTHGDILSGDGAVLGNGNTVNNG
                     DIHTGSASTVTVGDGNATGNTSAGGDVLSSHDGTVIQTSGHGTTSVSDGNTTTVHGNQ
                     TITDIHGTGNTSGVDNSSTPTHTSTIDNSEHDSSTHSIVDTSVHDNTVHDGSVHDSSI
                     HDTSLHDNSIHETTHTDTGLDAHLGF"
     gene            complement(4622..4762)
                     /locus_tag="VFC01_21830"
                     /pseudo
     CDS             complement(4622..4762)
                     /locus_tag="VFC01_21830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(4825..5235)
                     /locus_tag="VFC01_21835"
     CDS             complement(4825..5235)
                     /locus_tag="VFC01_21835"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HZN82300.1"
                     /translation="MGRRRILLVVTVFVLLAGCGGGRTVEVGVTAGSVQLAAGEVLRV
                     DLGQVNSSIGDSWYLVGTPDAAVLTGGEEDYDSDCDRDAVGCGGRLAWVFTARGPGKT
                     TLVFQYCYRSSLDACQPETSRGPQEPVQLAVSVN"
     gene            complement(5343..6362)
                     /locus_tag="VFC01_21840"
     CDS             complement(5343..6362)
                     /locus_tag="VFC01_21840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN82301.1"
                     /translation="MDRIPTHSVCVLATVLAVVGSLALGPVPKATASPAATGDFSDLV
                     DIGGRSLYLECRGQGSPTVILEAGAGGRADVWSQDLEEPEGTRTMVLPGVAAFTRVCA
                     YDRPGTLTEPNPSLDPHGPLFYPSRSDPVPQPRTTQDRVNDLHALLGAAAIPGPYVLV
                     GHSAGGLVTRLYASEYPDEVVGMVLLDSTHEDVWLRFQEALPPDQWAEFEALTVTNQD
                     LLDAYPEAERWFTAPLGNDATTGQVRQARKDAPLRPMPLVVLAHGIPFAPPFPGWPSE
                     KMEAIMTALQQDLAQLVPDARLIVATESGHNIHQDQPALVIDAIQQVVSAVRDPRSWV
                     ASGGG"
     gene            6810..7115
                     /locus_tag="VFC01_21845"
     CDS             6810..7115
                     /locus_tag="VFC01_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82302.1"
                     /translation="MKKFGIATAMVGGAAAALIGLAAPAAAAPTGPGDAQQTIDVLRA
                     QGYTVIVNRIGTAPLDQANVVAVRPGQHFSRTDTGVPGARNDIYTTVIDKTVYLDVK"
     gene            7181..8131
                     /locus_tag="VFC01_21850"
     CDS             7181..8131
                     /locus_tag="VFC01_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZN82303.1"
                     /translation="MIELARYQEGPSNVVVSRDGDDLVFWSSCHDYGRTITEESRIRI
                     ADFIAKGEGPWPWFDLRDKRDGALLVLNALGVNPPAWTTALPADVLDLFRRAERGSTD
                     VIELLACGAEPDPVDPCAASPLWYATRSGGSGIVVALIDAGADAGRRIDLSAQGDRFT
                     TILHEIVRLGRTVALAHALKKGTHPDLKDSDGATPLHVINGNNDNVNPEVVRSLVRAG
                     AAVDAAMPSGTQPIEHAVRRILPATVAAMVELGADPVRGLDALLPWWATVGTRYAGYR
                     AGDVADVIDILRAGGAEVTDRHRELAAGAGASQVEAALRR"
     gene            8142..8909
                     /locus_tag="VFC01_21855"
     CDS             8142..8909
                     /locus_tag="VFC01_21855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZN82304.1"
                     /translation="MARYSRYVAIGDSQTEGLWDGDDTVGLRGFADRLAELVDAHDPG
                     LTYANLAVRGRRIRDLLDDQLPQALAMKPDLVTVCIGMNDVTRPGPYFGHALAELEDL
                     YAQLALSGATVVTTTFPDLAQIIPIGRILAARVLRINDEIRRATDWYGFRLVDLYDAP
                     SMGHPDTWSTDRVHGSPKGHALFAAAAAEALELPGSNHDWAEIGGPVRQSLRSQAYSQ
                     ALWTQNMLMPWLWRHVRGRSAGDGRMPRRPRLEALPT"
     gene            complement(8917..9273)
                     /locus_tag="VFC01_21860"
     CDS             complement(8917..9273)
                     /locus_tag="VFC01_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN82305.1"
                     /translation="MQRRRTAFALGVVASSLVWAAPVQADTTDDAFLSALTDAGIGTA
                     DPANAAALGQQVCPMLAQPGQTAADVAAQVADAGGMSLGPATMFTGIAISLFCPTAVS
                     SVANGQSPIPLPFLGI"
     gene            complement(9289..9852)
                     /locus_tag="VFC01_21865"
     CDS             complement(9289..9852)
                     /locus_tag="VFC01_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82306.1"
                     /translation="MDADNYMTYEQFGRKFFEIAVSEDRVCRAIAAIAGDAFEMGPMG
                     QGPGKIAKVTAKVRIMEPRVTRTVGEAITFDIRIPLEIDMVIDLRIDKPKFMVFGEIA
                     LPATARAAEPLLLILDVEKPRPSDITIHVTSKSLRAEVLRVVAGVDAEIRRFISAHVS
                     GEIDSPASQKAKIIDVADSIDAAWTGI"
     gene            complement(9977..10777)
                     /locus_tag="VFC01_21870"
     CDS             complement(9977..10777)
                     /locus_tag="VFC01_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketosteroid isomerase family protein"
                     /protein_id="HZN82307.1"
                     /translation="MAFTRADMLATAERSPAAAGARDRTAWIGLFAATGRVEDPVGST
                     PHTGVAAIGRFYDTFIGPRNVRFHPDVDIVVGPTVVRDGELEVTMASTLTLRVPVYIR
                     YDLQDDAGELKIAALSAFWELPTMVGQFLRGGIRAVPAGLQLSKLLLTNQGLVGTLGF
                     LGGFRGIGSGAKGVVERFLDAACAGDEVGMRRRLAGRVRICRGDDLPMTAADLLRHLA
                     GARWHKLIGSGYAVVAAIERAGQRSVIFTDLGSEPVAITRIRVFSEAG"
     gene            10886..11284
                     /locus_tag="VFC01_21875"
     CDS             10886..11284
                     /locus_tag="VFC01_21875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82308.1"
                     /translation="MSSNGPFGFDPEEFDRVVREAGEGLRDALDGVSRFFTTSGERAG
                     WATVFDEFARHSRGRTEPETTGETGDGVWAIYTVDDDGGAHIEQVYPTELDALRANKT
                     NTDPTRKVRFLPYGIAVSVLDASDVSDATE"
     gene            complement(11285..11740)
                     /locus_tag="VFC01_21880"
     CDS             complement(11285..11740)
                     /locus_tag="VFC01_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN82309.1"
                     /translation="MSWWVARADRSVSEQIPAPPDEVRDFYVDLNNIRRVHPLVVAVR
                     ATDRRQTADGYVQSYRVQDRIPLGPLHLRISYVARLHVPNIGDVTAEARQFPRVRLRT
                     TVTFEPVDAGTRITEHMCIDAPRPLAAMTVREAVKAHTAMLAGMRRCFE"
     gene            complement(11737..>12076)
                     /locus_tag="VFC01_21885"
     CDS             complement(11737..>12076)
                     /locus_tag="VFC01_21885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZN82310.1"
                     /translation="PGDPAWARNDPTVQAGRLAGSGIRLWVYCGSGTPTDPALASPDA
                     PIGGLGFLEGMAIDSNHAFADAYIAAGGNNATFNFPGGIHAWGYSGQQLQQMKPDIQR
                     VLGAAPAAQA"
BASE COUNT         2084 a         3967 c         4047 g         1978 t
ORIGIN      
        1 gccttggcgg cgtacccgct caactggatg ttgagcgggt tggcgaccgc gtagtgcgga
       61 ccgaggtcga ccaccaatcc catgttgcgg tgcttgtagg tcttcatcgt gccgtagccc
      121 aggctcgctg cgacgttgcg ccccaacagc tttccctgcc ggatcgcgtg ctgtgcggtg
      181 ggcggggtga ccttgcccgg ctgggtgaca tcgggcacgg cggcggcatc ccctgcggag
      241 aacacgtccg gttggccggg cacccgcagg tcggtttcca ccttcagcct gcccttctcg
      301 gttggcaggc cgagcttttc gatcagcggc gcgccggtca cgccggtgac ccaggcgacg
      361 gtgtgggtgc ggatcagcga atcatcgctg agcacaacgt gttcggagtg gacttccttc
      421 agcgtgatgc cgagccggac gtcgatgccg cgtgattgca gcacccgctg cgctgcggca
      481 ccgagcttct ccccgacttc gggcatgact tgttcggcca ggtccagcag gacgaacttg
      541 acgtcggccg gatcgaagtt catctgcttg gcggcggaat cggcgagcgc ccgcagttgg
      601 acggccagtt cggtgcccga atacgacgcc ccgacgacca ctatcgtccg gcgcgccgca
      661 gcccggccgg gatcgtcgtc gacatcggcc agttcgagtt gctcgagtac gtgatcgcgc
      721 aggaacagcg cctcggccgt cgacttcagc ccgcgtgcgt gctcagcgag accggggacg
      781 tcgaacagtt tcgtcaccga cccgggcgtc aggacaaggc ggtcccacgg caaggtacgg
      841 gcgcgctcct cgggatcggt gaaggtgacg gtgcgtccgt cgaagtcgac gccgtcgacc
      901 ctgccgcgga tctgctgcac gtcccgcagc gcgttggcaa gtggaatcgc gacgaaacgg
      961 gcgtcgacca gtccgccggc cacgtcggga agcagcggtg tgtacagcat gtagtcgacc
     1021 ggcgaaatga tcgagatgtc ggccgaggcg ttgtgtttac ggagctttcg ggccagatga
     1081 cgggcgcact cgaaaccggt gaacccgctg ccgacgatta ccacagaatt cggctcctcg
     1141 cgcgagcgct cgtcggtggc catcgcgccc cactgtacgt gctacggggt ctccagcagc
     1201 gtcccgaacc tcatcgccag ccacggcttg cggccgccgg gccggagctg cccgcgcagc
     1261 acgggcgaca gctggccgat cctgccgtag ccaagcagca ggaaggcgac cgggtccgcg
     1321 gtgatgtggc agtcggcctt ctctcctgcg gcggtgacga ccgcggtgcc gtcgtcgacc
     1381 gtcaatcggt agctggcccc gccgcgcatc cgcagctcga agctgatgcg aacgccggcg
     1441 gcgcgctgcg gccgcaagta ttgcggcgca acggtcagca cgcccggaat caccaggaac
     1501 gcgtcgtcgg cggcgatcgg ccaccggatc ttggccgtgc gggagatatc gaggccgtgg
     1561 atgagctgct caccgagcag cagggccgcc atggtgggcg gaccgatcac caaaccgttg
     1621 ggggtggcga tctcggcgct caggtcggcg ttggcagcgg cgttcaggta cgcgtcggcg
     1681 gcgacctcga gcatgtcggc caggcgggac aggttgcgct ctcggatctc ttcgaggtgg
     1741 cgggcgttga ccttcgcgct gagccgtgag ggcgattcgg tcggccgatt ggcgtgggtc
     1801 atatagccac cggtgtgacg tgtcagtgcc tgcgtgtagt cacggagatc accgacgacg
     1861 tgtgcggcgg tctcggcggc ggtccactgc aggccgggcg cgggtgcgtc gggcttgtcg
     1921 atgtttcgcc acaagtcggc gcttcgtgcc acggcgatgc gcagcgcgtt gagggtgggg
     1981 cggtaggcgc gacgggtgtc ctcggccggc ccggttaggt gcacgggggc agatgtcata
     2041 gggtaactat ttcacataat gatgctatgt caatagggta tcgtgggtct atcgaagagg
     2101 agcaatgcat gtcggacccg cccgcctcgc tgcgcgccga acaggtcgcg cagacgcgag
     2161 cggcgttggt cgcggccggc cgccgactgt tcggcgagaa gggtttccgc gcgacctctg
     2221 tcgaggacct ggcgcgcgaa gcccgcgtca cgacaggggc tctttatcac catttcccca
     2281 ataagacagc gcttttcgag gccgtgttct tgcaggcgca cacggatttg atgacggcgt
     2341 ccaccaaggc ggcgcgcgga gcgtcagacg gcatcgacga gctggctcgc ggcttcgagg
     2401 cgttcctcga cggcgtgctg cagccggata tgcagcgcat cctgatcgtc gacgggcccg
     2461 cggtgttggg gctggcgcgc tacaccgaac tcgatgagca atacgcccac gcggcgatcg
     2521 tgcacgcgct gacggccgcg gcgcaggcag ggacgatcac ggtcgaggat cccgagaccg
     2581 ccacgcggct gctgctcggc gcgctcaccc gcggcgcgat gctgatcgcc aacgccgccg
     2641 atcctgttca gacgcggcac gctgtcgcca agtcgatgcg ggcactgcta tccagcttca
     2701 ctccgtagcg cggatgacgg atgctggacg catgacgcgt ccgcgacctc tgcgccgatt
     2761 cgacccgttc cggccgatcg acatgctggc cgcgctgtgg tcttcggcgg cggtagcgcc
     2821 ggtcagctcc ggggcggcgg cggcctaccg gacgctgttc atgacggtgc gtcggcttgt
     2881 ggtgggtcgt cggttggcgg tccggctgga ggacggcgat ctcacgctga cggtcacgga
     2941 gttcgattct cggctggatg tgcgagcgct gtcggtgggc caactcaacg atgtccggct
     3001 ggcggcgcgg gacatctgtt ggcacgggag tcgattcgat cgggcgacgg cggtgctgca
     3061 caacgtgcac atgaagccga cggcgccgcc ggtgctcgtg gccgctccgg tggagctgac
     3121 gctcgagatt ccggcgccgg tgctcgatga tttgttcagg tgggcggcgc cgcgattgtc
     3181 gggcgaggtc ggtgccgatg cgatcgcgcg gctgcgactg gcgagcaggc cgctgctcgg
     3241 tcacatcgaa gtcgatgcca gcctcgacgg ttcgacgttg tggctgaagc cgcggggcct
     3301 cgccgtccgc cagcgacatt ggcggttgcc ggcacgcacg ccggcctatc cggtgcacct
     3361 ccccgagctt ccgcaggggc tgtcattgac cggtatcagc tttgcgcccg gcgtggtgtg
     3421 cctgtcgggc acgctgccgg aatggcggat ggatgtgccg cgaaaacgct tggaggacat
     3481 catcaatcag ctcagcgtgg tcggtgtgcc gctgaatctc accaggctgg ggcgcctctt
     3541 ctaatcgcgc cgttgcactg gcagtaagtc catggctgtg tgacaccgaa ttgccagaaa
     3601 acgtcactac cgttcgagcg cgtgtcgggg ccacgattgc tgacttcatg agaggcgcgc
     3661 catgatcaat ctgctcgact acatcctcga tctgttccgg gacgagagcc acgctcaggc
     3721 gttcgtcgca aatcccgacc aggcgatggc cgacgcaggc ctgtcgacag tcaattccgc
     3781 ccagctgcag tcggtggcgg cgacggcaat cccaagcctg gcgatgggcg gcagtggtga
     3841 cgcggtgacc gatctgcagc aggccgtgtc gagttattac ggattccaac cgcagcccga
     3901 cgtcgcgccg cagaccgact tctcccccag caccgacctg gcgagcaaca acagcttcat
     3961 gagcccgacc accaacatca ccgacgacca cagcgtcagc ttcggcatgg gcgatatcac
     4021 cctgggtgac aagacaactg cgcagggcga tggcgccgtc gctgtcggcg gaagcacgca
     4081 cggcgacatc ctcagcgggg acggcgcggt gttgggtaac ggcaacacgg tcaacaacgg
     4141 cgatatccac accggatctg cctcgacagt gactgtcggc gacggcaacg ccaccggcaa
     4201 cacgtcggcg ggcggcgacg tgctcagctc gcacgacggc acggtcatcc agacgtcggg
     4261 ccacgggaca acctcggtgt cggacggcaa cacaaccacg gtgcacggaa atcagacgat
     4321 caccgacatc cacggcacgg gcaacaccag tggcgtcgac aacagttcga cgcccactca
     4381 cacgtcgacg atcgacaact ccgagcacga ttcatcgaca cactcgatcg tggatacttc
     4441 cgtgcacgac aacacagtgc acgatggttc cgtacacgac agctcgattc acgacacctc
     4501 gctgcacgac aactcgatcc acgaaaccac ccacacggac acgggtttgg acgcccatct
     4561 cgggttctga cacaccccta gaacgggttt ggcggtgagc caggctgggt tgcgtcgacg
     4621 cgggacacag cgcgccgccg tgtgcctctg tgacgtaggt gtgtccggtc gcggcggtga
     4681 gaatcactgt cccgtagggc aattgctgat cagtccagcc acccgggtcg ctgtggaagg
     4741 tcttgatcac atggtgacac cggagcggtg gagaacgctt cagagcgttt gcgatcgtat
     4801 gaaccatgga ggctcaccta ggctctagtt cacggagact gcgagctgga ccggctcctg
     4861 cggccctcga ctggtctccg gttgacaggc gtccaggctg gaccgatagc aatattggaa
     4921 taccaaggtg gtcttgcccg gaccgcgcgc ggtgaaaacc cacgccaggc ggccgccaca
     4981 gccgaccgcg tccctgtcgc agtcggagtc gtagtcctct tcgcccccgg tgagcaccgc
     5041 ggcgtcggga gtacccacta ggtaccagga atccccgatg gacgagttaa cctgccccag
     5101 gtcaacccgg agaacctcgc ccgcggcgag ctgaaccgag ccggcggtta cgcccacttc
     5161 gaccgtccgt cccccgccac acccggcgag caggacaaac actgttacca ccaaaaggat
     5221 tcggcgacga cccatgccgc tgaacgtacc gttattgcga acaggcgaac tgccttcagt
     5281 agatggtggt aacggatacg cgaagaacgt ttcgagctcc gagtgaaagg ccagtcggca
     5341 acctacccac ctcccgacgc cacccacgag cgcgggtcgc gtacggccga aaccacctgc
     5401 tggatagcgt cgatcaccag cgctggctgg tcttggtgga tgttgtgccc gctctcagtg
     5461 gcaacgatca ggcgagcgtc ggggacgagc tgggcgagat cttgttgcag cgcggtcatg
     5521 atggcttcca tcttctcgct cggccagccg ggaaagggcg gggcaaaagg gatgccgtgg
     5581 gcgagaacga ccaagggcat cgggcggagg ggagcatcct tgcgcgcctg gcggacctgg
     5641 ccagtggtgg cgtcgttgcc caacggggcg gtgaaccacc gctccgcctc cggataggca
     5701 tcgaggaggt cttggttggt gacggtgagc gcttcgaact ccgcccactg gtccggcggc
     5761 agggcctcct ggaaacgcag ccacacatcc tcgtgagtcg agtccagcag caccatgccg
     5821 accacctcgt ccgggtactc gctggcatac agccgcgtca ccagaccgcc cgcggagtgg
     5881 ccgaccagga cgtagggacc ggggatcgca gcggcgccca gcagcgcatg caggtcattg
     5941 accctgtctt gcgtagtgcg cggctggggc accgggtcgc tgcggctggg gtagaagagc
     6001 ggcccatgcg gatcgagcga gggattgggc tcagtgagcg tgcccggacg gtcgtaggca
     6061 cagacgcggg tgaacgccgc cacaccgggc aggaccatcg ttcgcgtgcc ctcgggctcc
     6121 tccagatcct ggctccagac gtcggcccgg ccgccggccc cggcttccag gatcacggtg
     6181 ggactgccct ggccgcggca ctccaagtag agcgatcgac cgccaatgtc gaccagatcc
     6241 gaaaaatcgc ccgttgccgc cggcgacgcg gtggctttcg gcacagggcc gagggcgagg
     6301 ctgccaacaa cggcgagcac ggttgccagg acgcacacgg agtgagtggg aatgcgatcc
     6361 ataccaaatc ctccttggca ggcgccgcct ccaaccatca gcgaggatca gcttcggagg
     6421 gcagcgacgc ctatcgtgca ccctgtttgt gataggtgcg gccgcattcg gacataacgg
     6481 ccagcagccg gatgatttcc tttttggtct tgccttcttg ggttccgcgc caagtagcgc
     6541 tgggtgggtt cgtgctatcg cagcctgttg acggcgatca gatggatcga gctgtcggcg
     6601 tcgcggtctc cgccgcggtg caggggcgtc ggtttcaaaa tctcacaggc cgaccgcgca
     6661 cgtcgtcttc gaagcgaagg acgaaacctc tgcacctaac tccaagtgtg gatgtcagct
     6721 gtgagagcca tcaccgcagg ccgcatcccg gaatcaaatc gctgaccagc agtgtttaaa
     6781 ggtctagaca tatgagagag gacccaaaga tgaagaagtt cggaattgcc actgcgatgg
     6841 tcggtggcgc cgccgctgcg ctcatcggac tggcagctcc ggccgccgcc gcgccgaccg
     6901 gccccggcga cgcccagcag accattgacg tgctgcgagc ccagggctac accgtgatcg
     6961 tcaaccgaat cggtaccgcg ccgctcgacc aggccaacgt ggtagccgtc cggcccgggc
     7021 agcacttctc acgcacggac accggcgtac ccggcgcccg caacgacatc tacacgaccg
     7081 tcatcgacaa gacggtgtat ctagacgtca agtaggccaa tacatgagga ggggcatcca
     7141 attgggtgcc ccttcttcgt atttggacga tgctgaagcc gtgatcgaac tggcgcgcta
     7201 ccaggagggg ccgtcgaacg tcgtggtgtc gcgcgacggc gacgatctcg tgttctggtc
     7261 gtcatgccac gactacggca ggacgatcac cgaagaatcc cgcatccgga tcgccgactt
     7321 catcgccaag ggcgagggac cgtggccatg gttcgacttg cgcgataaac gtgacggggc
     7381 gctgctggtg ctcaacgcgc tcggcgttaa cccgccggcc tggacgacgg cgttgccggc
     7441 agacgtcctc gatctcttcc gccgtgccga acgcggcagc acagacgtga tcgaactgct
     7501 cgcctgcggc gccgagcccg atcccgtcga tccctgtgcg gcgtcaccac tttggtacgc
     7561 aacgcgctcg ggcggctcag gcatcgtcgt ggccctgatc gacgccggcg ccgatgccgg
     7621 ccgccgcatc gacctgtcgg cccaaggcga ccgtttcaca acgatcctgc acgagatcgt
     7681 ccgactcggc cgcactgtgg cgctcgcgca tgccctgaaa aagggaaccc acccagacct
     7741 caaagattcc gatggcgcca cgccactgca tgtcatcaac ggcaacaacg acaacgtcaa
     7801 tccggaggtg gtccgctccc tcgtacgcgc cggcgccgca gtcgatgccg cgatgccaag
     7861 cggcacccaa cccatcgaac acgcggtccg gcggatcctg cccgcgacag tcgcggcgat
     7921 ggtcgaactc ggcgccgacc cggtccgcgg cctcgatgcc cttctgccct ggtgggccac
     7981 agtcggaacg cgctatgcgg gctaccgggc aggcgacgtc gccgacgtca tcgacatcct
     8041 gcgtgccggc ggcgccgagg tgaccgaccg gcaccgcgaa ttggcggctg gcgccggagc
     8101 gtcgcaggtc gaagccgcgc ttcgccgcta aagtcgccgc tgtggcgcga tactcacggt
     8161 atgtcgcgat cggcgacagc cagaccgagg gcctatggga cggcgacgac accgtcggcc
     8221 tgcggggatt cgccgaccgg ctggctgagc tcgtcgacgc gcatgacccc gggctgacct
     8281 acgccaatct tgccgtgcgc ggccgtcgca tccgtgacct cctcgacgat cagcttcccc
     8341 aggcactggc tatgaagccc gatctggtca ccgtctgcat cgggatgaac gacgtgacgc
     8401 ggccggggcc ctacttcggt cacgcgctgg ccgagctcga ggacctgtac gcccagctgg
     8461 cgctgtcggg ggcaaccgtc gtcaccacga ccttccctga cctcgcgcag atcattccaa
     8521 tcggccggat cctcgcggcg cgtgtgttgc ggatcaacga cgagatcagg agggcgacgg
     8581 actggtacgg cttccggctg gtcgacctct acgacgcgcc ctcgatgggc cacccggaca
     8641 cctggagcac cgaccgcgtg cacgggtcac cgaagggaca cgccctgttc gccgccgcgg
     8701 ccgccgaggc gctggaattg cctgggagca accatgattg ggccgaaatc ggcgggcccg
     8761 ttcgtcagtc gctccggtcg caggcgtact cgcaggcgct gtggacccag aacatgctca
     8821 tgccgtggct gtggcggcac gtgcgcggcc ggtccgctgg agacggaagg atgccgcggc
     8881 ggccgcgact tgaggcgctg cctacttagc tgcgacctaa atgcccaaga acggcagcgg
     8941 gatcggcgat tggccgttgg cgaccgacga cacggccgtc gggcagaaca gcgagatcgc
     9001 gatgccggtg aacatggtcg ccgggcccag cgacatcccg cccgcgtcgg caacctgggc
     9061 ggcgacgtcg gcagcggtct gaccgggctg ggccagcatc gggcagacct gctgacccaa
     9121 ggcggcggcg ttcgcggggt cggcggtgcc gattccggcg tcggtcagcg ctgacaggaa
     9181 cgcgtcgtca gtcgtgtcgg cctgcaccgg cgccgcccac accaggctgg aagccaccac
     9241 accgagggca aaggcggttc gtctgcgctg catcggggga tcgtagcgtc agatacccgt
     9301 ccaagccgca tcgatgctgt cggccacgtc gatgatcttg gccttctggg acgccgggct
     9361 gtcgatttcg ccggagacgt gggcagagat gaaccgcctg atctcggcgt cgacgccagc
     9421 aacgacccgc agcacctccg cgcgcaacga cttggacgtg acgtgaatgg tgatgtcgga
     9481 cgggcgtggt ttctcgacgt cgaggatcag cagcagcggc tcggcggccc gcgcggtggc
     9541 gggcagcgcg atctcgccga acaccatgaa tttcggtttg tcgatccgca ggtcgataac
     9601 catgtcgatc tcgagcggga tccggatgtc gaaggtgatc gcctcgccca ccgtgcgggt
     9661 cactcggggc tccatgatcc ggaccttggc ggtcaccttg gcgatcttgc ccggcccctg
     9721 acccatcggg cccatctcga atgcgtcgcc tgcgatggcg gcgatcgctc ggcagacacg
     9781 gtcttcgctc accgcgatct cgaaaaactt gcggccgaat tgttcgtagg tcatgtagtt
     9841 atcggcgtcc atggtgctgt aaccgtctca cgtccgacga tccgactggg gcaacgcgac
     9901 cgaatcgcaa caccctagtc ccggccggcg cccgctcatg cgcacggttt acccgacgga
     9961 cacctggtga aacgactcac cctgcctcac tgaagacgcg aatccttgtg atggcaacgg
    10021 gttcggaacc caggtccgtg aagatcacac tgcgctgccc ggcacgctcg atggccgcaa
    10081 cgaccgcata cccggagccg atgagcttgt gccaccgcgc tccagcaaga tgcctcagca
    10141 ggtctgcggc ggtcattgga aggtcgtcgc cgcgacagat gcgcaccctg ccggcgagtc
    10201 gtctccgcat gccgacctcg tcgccggcgc atgcggcatc caggaatcgc tcgacgacac
    10261 ccttggcccc gctgccgata ccgcggaatc cgccgagaaa tccgagcgtg ccgaccaacc
    10321 cctgattggt cagcaggagc ttcgatagct gaaggccggc gggcacggcc cggattccgc
    10381 cgcgcagaaa ctggcccacc attgtcggca gctcccaaaa agctgacagt gcagcgattt
    10441 tcagctcacc cgcatcgtct tgcaggtcgt aacggatgta caccggcacc ctcagggtga
    10501 gtgtcgacgc catagtcacc tcgagctccc cgtcccgaac gacggtcggg ccgaccacga
    10561 tgtcgacgtc cgggtgaaac cggacgttgc gcggaccgat gaaggtgtca tagaagcggc
    10621 caatcgccgc cactcccgtg tgcggcgtgg atccgaccgg gtcctcgacg cgtccggttg
    10681 cagcgaaaag gccgatccag gccgtgcgat cgcgggcgcc cgcggccgca ggcgaacgct
    10741 cggcggtcgc cagcatgtcg gcgcgagtga aggccatacg gcttgcctac cacctgcggt
    10801 tggcctcggt cgactacacg ccaacacgca ggcacactgt ggttaaccgc aatgcagcgg
    10861 tgcacggcaa tcaggaggaa ccgctatgag cagcaacggg cctttcgggt tcgaccccga
    10921 agagttcgat cgcgtggtcc gcgaggcggg cgagggcctg cgcgacgcgc tcgacggagt
    10981 cagcaggttc ttcaccacct ccggtgaacg ggccggatgg gcgacggtct tcgacgagtt
    11041 cgcgcggcat tcaaggggca gaaccgagcc ggagacgacg ggcgagaccg gcgacggcgt
    11101 gtgggcgatc tacaccgtcg acgacgatgg tggcgcccac atcgagcagg tgtatccgac
    11161 ggaactcgat gcgcttagag cgaacaagac caacaccgat ccgacgcgca aggtgcgatt
    11221 cctgccgtat ggcatcgcgg tcagcgtgct cgacgcatcc gacgtatccg acgcgacgga
    11281 atagctactc gaagcagcgg cgcatgcccg ccagcatggc ggtgtgcgcc ttgactgcct
    11341 cgcggaccgt catcgccgcc aagggacgcg gcgcatcgat gcacatgtgc tcggtgatgc
    11401 gcgtgcccgc gtcgaccggc tcgaacgtca ccgttgtacg cagtcgaacc cggggaaact
    11461 gacgcgcctc ggccgtcaca tcgccgatgt tcggcacgtg caggcgcgcc acatagctga
    11521 tccgtaagtg caagggcccc aacggaatcc gatcctggac tcggtagctc tgcacgtagc
    11581 cgtccgcggt ctgccgacgg tcggtagcgc gcaccgccac gaccaacgga tgcactcgtc
    11641 tgatgttgtt caggtcgacg tagaagtcac ggacctcgtc cggcggcgcc gggatctgct
    11701 ccgagacact gcggtccgcg cgcgccaccc accaactcac gcctgcgcgg cgggcgcggc
    11761 gccgagcacc cgttggatgt cgggcttcat ctgctgtagc tgctggcccg agtaacccca
    11821 cgcgtggatg ccgccgggga agttgaacgt ggcgttgttg ccgcccgcgg cgatataggc
    11881 gtcggcgaac gcatggttgg agtcgatcgc cattccctcc aggaagccga gtccgccgat
    11941 gggcgcgtcc gggctggcca gcgccggatc ggtcggcgtg ccgctgccgc agtagaccca
    12001 cagccggatg ccgctgccgg ccagcctgcc cgcctggacg gtgggatcgt tgcgggccca
    12061 ggccgggtca cccggc
//
LOCUS       DASRJA010000495         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2952935,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000495 DASRJA010000000
VERSION     DASRJA010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2952935"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3351)
                     /locus_tag="VFC01_21890"
     CDS             complement(<1..3351)
                     /locus_tag="VFC01_21890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HZN82311.1"
                     /translation="MVSDPIGRFSALTREWFAGTFAAPTAAQAEAWSAIADGDNTLVI
                     APTGSGKTLAAFLWAIDRLASSEPRPARAGTRVLYVSPLKALAVDVERNLRTPLTGIG
                     RVAERRGVPAPDISVGVRSGDTPPAQRRTLITNPPDILITTPESLFLMLTSAARETLA
                     EVQTVIVDEVHAVAGTKRGAHLALSLERLDQLSASPAQRIGLSATVRPPEEVARFLSG
                     QARTTIVAPPAAKTFDLSVQVPVPDMANLENNTIWPDVEARIVDLIEAHRSSIVFANS
                     RRLAERLTSRLNEIHAERSGIELPSGRNPQVGGGSPAQLMASGQSHGAPTLLARAHHG
                     SVSKEQRAQVEDDLKTGRLRAVVATSSLELGIDMGAVDLVIQVEAPPSVASGLQRIGR
                     AGHQVGEISQGVLFPKHRTDLIGCAVTVKRMLTGEIETMHVPTNPLDVLAQHTVAACA
                     LEPIDADRWFDAVRRSAPFATLPRSAFEATLDLLSGKYPSTEFAELRPRLVYDRDAST
                     LTARPGAQRLAVTSGGAIPDRGMFTVYLATDSEKPSRVGELDEEMVYESRPGDVISLG
                     ATSWRITEITHDRVLVIPAPGQPARLPFWKGDGVGRPAELGAAVGAFTGELARLGREE
                     FVARCRAMGFDEFATDNLWQLLDDQRQATGTVPSDSTFVVERFRDELGDWRVILHSPY
                     GLRVHGPLALAVSRRLRERYGIEEKPTASDDGIIVRLPDTEDAAPGADLFVFDADEIE
                     PIVTAEVGGSALFASRFRECAARALLLPRRHPGKRSPLWHQRQRAAQLLDVARKYPDF
                     PIVLETVRECLQDVYDVPALTELMHRVAQRRLRVVEVETATPSPFAASLLFGYVGAFM
                     YEGDSPLAERRAAALSLDSVLLAELLGRVELRELLDPQVIATTMQQLQHLSSDRLARD
                     AEGVADLLRVLGPLTEHEIAERCTTAEVGGWLDGLQAAKRALTVSFADQTWWVAVEDI
                     GLLRDGVGVAVPVGVPAAFTESVADPLGELLGRYARTHGPFTTHDAAARFGLGLRVTA
                     DVLGRMDIDGKLIRGEFTDSADGEQWCDADVLKILRRRSLAALRAQIEPVSTAAYGRF
                     LPAWQQVGRHNSGIDGLAAVIEQLAGVPL"
BASE COUNT          553 a         1186 c         1106 g          516 t
ORIGIN      
        1 aagtggcact ccggccaact gctcgatcac cgcggccagc ccgtcgatgc cggaattgtg
       61 tctgcccacc tgttgccacg ccggcaggaa ccggccatag gcggctgtac tgaccggctc
      121 gatctgcgct cggagcgcgg ccaaggaccg gcgccgtaga atcttcaaaa cgtccgcgtc
      181 acaccattgt tcgccatcgg cggagtccgt gaattcgccg cggatcaact tgccgtcgat
      241 gtccattcga cccagcacgt ccgcggtcac ccgtagtcca aggccgaacc gggccgcggc
      301 gtcgtgagtg gtgaacgggc cgtgggtgcg ggcgtatcgg ccgagcagtt cgccgagtgg
      361 atcggccacc gattcggtga aagccgcggg caccccgacg ggcaccgcaa cgccgacgcc
      421 gtcgcgcagc aggccgatgt cttcgaccgc cacccaccag gtttggtcgg cgaaggacac
      481 cgtcagcgcc cgtttggcgg cctgcaaccc gtccagccag ccgccaacct ctgctgtcgt
      541 acagcgctcg gcgatctcgt gttcggtcag cgggcccagc acccgcagca gatccgcgac
      601 gccctccgca tcccgggcca atcggtcgga gctgaggtgt tgcagctgct gcatggtggt
      661 cgcgatgact tggggatcga gcaactcccg cagctcgacc cggccgagca gctcggcgag
      721 cagcacggaa tccagcgaca aagcggcggc acgccgctcg gccaaagggc tgtcgccctc
      781 gtacatgaat gcgccgacat agccgaacaa caacgacgcg gcgaacggcg acggggtcgc
      841 ggtctccacc tcgaccaccc gcaacctgcg ctgagctacc cggtgcatca gctcggtgag
      901 tgcgggcacg tcgtagacgt cctgcaggca ttcgcggacc gtctccaaca cgatggggaa
      961 gtccgggtac ttgcgggcga cgtcgagcag ctgtgccgcc cgctgccgct gatgccacaa
     1021 cggagaccgc ttgccgggat gccgtcgcgg caaaagcagt gcgcgggcgg cacattcgcg
     1081 gaaccgcgac gcgaacagcg ccgagccgcc cacctcggcg gtgacaatcg gttcgatctc
     1141 gtcggcatcg aacacgaaca ggtcagcacc cggtgcggcg tcctcggtgt ccggcaaccg
     1201 cacgatgatc ccgtcgtcgg aggccgtcgg cttctcctcg atcccatagc gctcgcgcag
     1261 ccgtcgcgac accgccagcg caagcggtcc gtgtacccga agcccataag gggagtgcag
     1321 gatcacccgc cagtcgccga gttcatcgcg gaatcgttcg accacaaacg tgctgtcgct
     1381 gggcaccgta cccgtcgctt gtcgctgatc gtccagcagc tgccacaggt tgtcggtcgc
     1441 aaactcgtcg aaacccattg cacggcaacg cgcgacgaac tcctcacggc ccagccgcgc
     1501 cagctccccg gtgaacgctc cgacggcagc gcccaattca gctggcctgc cgacgccgtc
     1561 gcccttccag aacggcagcc gcgcgggctg cccgggtgcc ggaatgacca gcacccggtc
     1621 gtgggtgatc tcggttatgc gccagcttgt cgcacccagc gagatcacgt cacccgggcg
     1681 tgactcgtac accatctcct catcgagttc tcccacccgc gaaggttttt cggagtcggt
     1741 cgccaggtag acggtgaaca tgccgcggtc cgggatcgca cccccagacg tcaccgccag
     1801 ccgctgcgct cccggacgtg cggtcagcgt gctggcgtca cggtcgtata ccagccgcgg
     1861 ccgcagctcg gcgaactcgg tggacgggta cttaccgctc aacaggtcta gcgtcgcctc
     1921 aaatgcgctg cgcggcaacg tcgcaaacgg agcactgcgg cgcaccgcgt cgaaccaccg
     1981 atcggcgtcg atcggctcca gcgcacaggc cgccacggtg tgctgggcca gtacgtccag
     2041 cggattcgtc ggcacatgca tggtctcgat ctcgccggtg agcatccgct tcacggtcac
     2101 cgcgcagccg atcagatccg tgcggtgctt gggaaacagc acaccctgcg agatttcgcc
     2161 gacctggtga ccggcgcggc cgatgcgctg cagtccgctg gccaccgacg gcggcgcctc
     2221 gacctggatc accagatcga ccgcgcccat gtcgatgccc agttccaggc tggacgtggc
     2281 gacgacggcg cgcaggcgtc cggtcttgag gtcgtcctcg acctgcgcgc gctgttcctt
     2341 gctcaccgag ccgtgatgcg cgcgggccag cagggtgggc gcgccgtggg actggccgct
     2401 ggccatcagc tgcgccggcg agccaccgcc aacctgaggg ttgcgcccgg aaggcagctc
     2461 gatgccggac cgttcggcgt gaatctcgtt gagccgcgac gtgagtcgct cggccagccg
     2521 ccgcgaattg gcgaacacaa tcgagctgcg gtgggcctcg atcagatcga cgatgcgtgc
     2581 ctcgacgtcg ggccagatcg tgttgttctc cagattggcc atatcgggga ccggaacctg
     2641 gacggacaga tcgaacgtct tggccgcggg cggcgcgacg atcgtcgttc tcgcctggcc
     2701 cgacaggaat cgcgccactt cctccggtgg tcgcacggtc gccgacagcc cgatccgctg
     2761 cgccggtgat gccgacagct ggtcgagccg ctccagcgac aaggccagat gtgcgccgcg
     2821 tttggtgccc gcgacggcgt gcacctcgtc gacgatgacg gtctgcactt cggccagcgt
     2881 ctcgcgtgcc gctgacgtca gcatcaggaa caacgactcc ggcgtggtga tcaggatgtc
     2941 cggcggattg gtgatcagcg tgcgacgctg cgctggcggc gtatcgccgg acctcacgcc
     3001 gacgctgatg tcgggtgcgg gcacgccccg gcgctcggcg acccggccga tgccggtcag
     3061 cggggtgcgc aggttgcgct cgacgtcgac ggccagcgcc ttcagcggcg acacgtacag
     3121 cacgcgggta ccggcgcgtg cgggccgcgg ttccgatgac gccagccggt caatcgccca
     3181 caggaatgcg gccaacgtct tgcccgaccc ggtaggcgcg atgaccagcg tgttgtcgcc
     3241 gtccgcaatc gccgaccacg cctcggcctg cgcggcggtc ggagccgcga aggtaccggc
     3301 gaaccactcg cgcgtcagcg cgctgaagcg gccaatcgga tcggaaacca tctagccatc
     3361 g
//
LOCUS       DASRJA010000496         2981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_358971,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000496 DASRJA010000000
VERSION     DASRJA010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358971"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(399..482)
                     /locus_tag="VFC01_21895"
                     /pseudo
     CDS             complement(399..482)
                     /locus_tag="VFC01_21895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772796.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
     gene            complement(525..2054)
                     /locus_tag="VFC01_21900"
     CDS             complement(525..2054)
                     /locus_tag="VFC01_21900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN82312.1"
                     /translation="MPEATHVDAQQRQVSGLAAAMTVIAMGLGYAISIGDPTSLSANL
                     QVVSAALQISGATATFVASLATLTMAATVLSAGALGDLYGMRRMYLVGLCGAIAFSGL
                     AAAAPIPAVMIVARAGVGVTLAFLIGLSLAITNAVFPPGRRAAAIAAYFGVGYAVATP
                     LPAISGVLAHALGWRACFFAVPLIAAVGFAITWRYVPETVRATRRLDLVGMALFAVAL
                     LGLIFGISRVETGINAIGVTAIAVGLVAGFAFVMQELRTQDPALDMRIFRSGRFNAAV
                     TAGVMFNIVLGGSMVLLAFYLVTIRKESHELFGLLLIPATAMAALAATAAGPAANRFG
                     PRTVLVSGLTVMLAGLLVLRSFDLNTSRTIVFVTAALIVVGGALVTTPQATIMMGSAP
                     ADLGGAVSAVKSAVNEGGYSLGPALFALVGINLFLTDSVHDLSKSGITRAETREALLT
                     AHGGREAHLADPEQARLVIDQAPQNAVDAIHTLSLVMAVGPIAAIILALWLIKPAGPG
                     R"
     gene            2211..2468
                     /locus_tag="VFC01_21905"
     CDS             2211..2468
                     /locus_tag="VFC01_21905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HZN82313.1"
                     /translation="MRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLAPSERR
                     GGGRYSVQATGAGGLQPGVDLSSNAAVAEAMDEGTSVDALR"
     gene            2455..2886
                     /locus_tag="VFC01_21910"
     CDS             2455..2886
                     /locus_tag="VFC01_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZN82314.1"
                     /translation="MRCVDTSVLVYAHRADLPEHADYRSLLERLANGDEPLGLPDVVL
                     SGFVRVVTNRRIFREPTNSDEVWQAVDALLAAPAAMMLRAGERHWVLFRQLAADINAR
                     GNDVPDAYVAAYAVENNATWLSADRGFARFPRLRWSHPMDL"
BASE COUNT          521 a          953 c         1020 g          487 t
ORIGIN      
        1 tgcccccgcc acctccgcct ccggtacttc ctgcgcccac ggcttccgcg tccggaggga
       61 ccggcgttgg ggtgaatacg cctttgggtt cggctgagag tggatttgcg accgacttgg
      121 gcgttggtct cccgacgctg cctccacctc ccccgccgcc acccctgccg tcggcctcgg
      181 ggtcggccca gacaaacctg ggcttcggct tctgacaggt gaatcgctcg acatgggccc
      241 cgcccaaaag gacggggccc atgttttggc accagcgcac tcaccggcag cattgattac
      301 cgccacttac tgttcctcag atgggttggg gcgcagtcga ttactgccgc cgtggcgtgc
      361 cgagctcgtc gaggtggtgg tggcccgtac gtcgacgatc atcgctcaaa gtgctgatag
      421 tccaacggac ttcgccacga gccgccccag tgccaaccac gatcggtgaa gacgcgcacc
      481 gctgccatgt gattccgctc gagacgagga ggtccgcgac cagatcatcg tccgggcccg
      541 gcaggcttga tcagccagag agccaagatg attgccgcta tcggccccac ggccatgacc
      601 aggctcaggg tgtgaatcgc gtcgacggcg ttttgcggcg cctggtcgat caccagtcgc
      661 gcctgctccg ggtcggccag gtgtgcttcg cgtccgccgt gggccgtgag cagcgcctcg
      721 cgtgtctccg cgcgcgtgat cccggatttg gagaggtcgt gcacgctgtc ggtaaggaag
      781 aggttgatac cgacgagggc gaagagtgcg ggcccgagcg agtatccgcc ctcgttgacc
      841 gcgctcttga cggcggagac cgcgccgccg agatccgctg gcgcgctgcc catcatgatc
      901 gtggcctgcg gtgtcgtgac cagtgcaccg ccaaccacga tgagcgccgc ggtcacgaac
      961 acgatcgtgc gagaagtgtt gagatcaaag gacctcagca ccagcaggcc ggcaagcatc
     1021 acggtcagcc cgctgacaag gacggtgcgg gggccgaacc ggttcgcggc cgggccggcg
     1081 gcggtggctg ccagtgcggc catcgcggtg gcgggaatca gtagtaggcc gaacagctcg
     1141 tgcgattctt tgcggatcgt gacgaggtag aaggcgagca ggaccatcga tccgccgagc
     1201 acgatgttga acatcactcc ggcggtcacg gcagcgttga accggcctga acgaaagatc
     1261 cgcatgtcca gcgccgggtc ctgcgtgcgc agctcctgca tgacgaacgc gaagccagcc
     1321 accaggccga cagcgatcgc ggtgacgccg atggcgttga taccggtctc cacacgcgag
     1381 atgccgaaga tcagccctag cagcgcgacg gcgaacagcg ccatgccaac cagatccagc
     1441 cgacgagtgg cgcgcaccgt ttcgggcacg taccgccagg tgatagcgaa gccaaccgca
     1501 gcgatgagcg gcaccgcgaa aaagcatgcg cgccaaccaa gtgcgtgtgc cagcacgccg
     1561 ctgatcgcgg gcagaggggt ggcgacggcg tagcccacgc cgaagtacgc cgcgatcgcc
     1621 gcggcccgtc gacccggcgg gaataccgcg ttggtgatcg ccagcgagag gccgatcaga
     1681 aacgccagtg tcacccccac gcccgcgcgg gcgacgatca tcacggccgg tatgggtgcg
     1741 gcggcggcca gcccgctgaa cgcgattgca ccgcacagcc cgacgaggta catgcgccgc
     1801 atgccatata gatcgccgag cgcgccggcg ctgagcactg tggcagccat ggtcagggtg
     1861 gccaggctgg cgacgaacgt ggcggtcgcg cccgagatct gcagggcggc cgagacgact
     1921 tgcaggttgg ccgacaggga cgtcgggtcg ccgatcgaga tggcgtagcc gaggcccatg
     1981 gcgatcaccg tcatggcggc ggcaagtccc gagacctgac gttgttgagc gtcaacgtgg
     2041 gtagcctccg gcacggctgg atgctaccgc cgcgagcatt cggccaaggg gactgtgcga
     2101 gccagttcac cgatccggat cttcatgttg aagtcgcaac ggcgacagcg aagcagatga
     2161 ggatcagatt gtggcatcta agcatcttag gctgatgcta agatgccagc atgcgcacga
     2221 cgatccgcat cgacgatgag ctctaccgcg aggtgaaggc gaaggccgct cgatccgggc
     2281 gcaccgtggc cgcagtgctt gaagacgccg tgcgccgtgg ccttgcgccg tccgagcgac
     2341 ggggcggcgg tcggtacagc gtccaagcga cgggtgcggg ggggttgcag ccgggcgtcg
     2401 acctctcgtc caacgccgcc gtcgccgagg cgatggacga gggtacttcg gtcgatgcgt
     2461 tgcgttgaca cgagcgttct cgtttatgcg caccgagccg atctccccga acatgcggac
     2521 tatcgctcgt tgcttgagcg gctcgccaac ggtgatgaac ctcttggtct gccggacgtc
     2581 gtgctcagtg gcttcgtgcg cgtggtgacc aatcgccgga tcttcagaga gccaacgaat
     2641 tccgatgaag tttggcaggc ggtcgatgcg ctacttgccg cgccggcagc catgatgctt
     2701 cgggcagggg agcgtcactg ggtgttgttc cgccagctcg cggcggacat caatgcacgc
     2761 ggcaacgacg ttccagacgc ctatgtggcc gcgtatgcgg tcgagaacaa cgcaacgtgg
     2821 ttgagcgccg accgggggtt cgcgcggttt ccccggctgc ggtggagtca tccgatggat
     2881 ctgtgatcta cggcgaggct gcgcgcgcag acgaccgcgc gccgagctcg gactggccgg
     2941 gccggttact cagctcctac tcgtcgcttt acgtccctgt c
//
LOCUS       DASRJA010000497         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3967234,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000497 DASRJA010000000
VERSION     DASRJA010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3967234"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1259
                     /locus_tag="VFC01_21915"
     CDS             15..1259
                     /locus_tag="VFC01_21915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN82315.1"
                     /translation="MKVLMVSWEYPPVVVGGLGRHVYNLATALAAAGHEVVVLSRRPT
                     GTDPSTHPSTDEIAENVRVVAAAQDPHEFDFGTEMMAWTLAMGHSMIRVGLGIKGRHN
                     KPWRPDVVHAHDWLVAHPAIALAEYFDVPLVSTIHATEAGRHSGWVSGSISRQVHAVE
                     SWLVHESDSLITCSASMSDEITELFGPGLTEIRVIRNGIDAALWPFAQRQPRSGPAQL
                     MYLGRLEYEKGVHDAIAALPRIRRTHPGTTLTIAGTGTQQQWLVEQARKHKVLKATTF
                     VGHLDHEELVRLLHTADAAVLPSHYEPFGIVALEAAATGIPLVTSNVGGLGEAVINNE
                     TGMSFAPRDVAGVAAAVRAVLDDPDAAQRMAIAARERLTSDFDWHTVADETAQVYLTA
                     KRREREPHRRRTVVERALPDRT"
     gene            1300..>2006
                     /locus_tag="VFC01_21920"
     CDS             1300..>2006
                     /locus_tag="VFC01_21920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN82316.1"
                     /translation="MDTKRFTLDTTLTLIAAYLGLFVGLIDANAINLALPAIRNDLGG
                     GISGAQWTIDAYNITFAAVLLTAGSLGDKFGRRTLLRIGLVTFVAASLVCAAAPSLPL
                     LLAARAIQGVGAATMLPQGLAITAAAFPNPVERARATAAWAMAAAISTAVGPILGGVL
                     TDTLGWRYIFWLNAPVGVAALLMSYRYLPESRDPDAARFDVPGQTLAMLGLGALTVVL
                     VEGRVMAPEWIIALAVAA"
BASE COUNT          338 a          724 c          617 g          327 t
ORIGIN      
        1 ggggtcgctc gcgcatgaaa gtcctgatgg tgtcgtggga gtacccaccg gtcgtggtcg
       61 gcggactcgg tcggcacgtc tacaacttgg cgaccgcact cgcggcagcg ggtcacgagg
      121 tcgtcgtgct cagccgcagg cccaccggca ccgaccccag cacgcacccg tcgaccgacg
      181 aaatcgctga gaacgtacgg gttgtcgccg ccgcgcagga cccacacgag ttcgacttcg
      241 gcaccgagat gatggcctgg acgctggcca tgggtcactc gatgatccgc gttggcttgg
      301 gaatcaaagg ccgccacaac aagccttggc ggcctgacgt cgtgcatgct cacgactggc
      361 tggtcgcgca tcccgcgatt gctctcgccg aatacttcga cgtcccactg gtttccacca
      421 tccacgccac cgaggccggc cggcattcgg gctgggtgtc cggttcgatc agccgccagg
      481 tgcacgccgt cgaatcctgg ctggttcacg aatccgattc gctcatcacg tgttcggcgt
      541 cgatgagcga cgagatcacc gaattgtttg gtcccggact gaccgagatc cgggtcatcc
      601 gcaacggaat cgacgccgcg ctatggccgt tcgctcagcg gcaaccacgc agcgggccgg
      661 cgcagctgat gtatctcggt cggctggagt acgagaaggg cgttcacgat gcgatcgcgg
      721 cgctgccgcg gatcaggcgc acccaccccg ggaccacgct gacgatcgcc ggcaccggca
      781 cccaacagca gtggctcgtc gagcaggcgc ggaaacacaa ggtgctcaag gcgacaacgt
      841 tcgtgggtca cctggaccac gaagagctcg tgcgcctgct acacaccgcc gacgccgccg
      901 tgctgccgag ccactatgaa ccgttcggca tcgttgcgct cgaggcggcg gcaacaggca
      961 taccgctggt cacctccaac gtcggcggcc tcggcgaggc ggtcatcaat aacgagaccg
     1021 gcatgtcctt cgcaccgcgc gacgtcgccg gagtagccgc agccgtgcgc gccgtgctcg
     1081 acgatccgga tgcggcgcag cgcatggcga tcgccgcccg cgaacggctc acctcagact
     1141 tcgactggca taccgtcgcc gacgaaaccg cacaggtcta cctcaccgcc aagcgccgcg
     1201 aacgcgaacc gcaccggcga cgcaccgtcg tcgagcgcgc acttcccgac cgcacatagt
     1261 tcgatcggcg tcgaatcatt ccgcgcctag cgtcggggta tggacacgaa aaggttcacc
     1321 ctcgacacga cgttgaccct gatcgctgcc tacctgggac tattcgtcgg cctcatcgac
     1381 gccaacgcga tcaacctcgc cctgcccgcc atccgcaacg atctcggcgg cggtatctcc
     1441 ggggcgcaat ggacgatcga cgcctacaac atcactttcg ccgctgtcct gctcaccgcc
     1501 gggtctctcg gcgacaaatt cggtagacgc acgctcctgc gcataggact cgtcaccttc
     1561 gttgccgcct cactggtctg tgcggccgcc ccgtcgctgc cgctgctgct ggccgcccgc
     1621 gccatccagg gtgtgggtgc ggccaccatg ttgccgcaag ggctggccat caccgccgcc
     1681 gcatttccca atcccgtcga acgggccagg gcgacagcgg catgggcgat ggccgcagcc
     1741 atcagcaccg ccgtcggacc gatcctcggc ggagtgctga ccgacacgct cggctggcgg
     1801 tacatcttct ggctcaacgc gcccgtcggc gtggccgcgc tgctgatgtc ataccgctac
     1861 ctccccgaat cccgcgaccc cgacgccgcg aggttcgatg tgcctggcca gacgcttgcg
     1921 atgctcggcc tcggcgcact caccgtcgtc ctcgtcgaag gccgggtcat ggcacccgaa
     1981 tggatcatcg cgctcgccgt cgccgc
//
LOCUS       DASRJA010000498         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4108684,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000498 DASRJA010000000
VERSION     DASRJA010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4108684"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /locus_tag="VFC01_21925"
     CDS             <1..1684
                     /locus_tag="VFC01_21925"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN82317.1"
                     /translation="AVERAPSPTTIREMTPERIYDSLATGSMQAQSTGLSDAQKKALA
                     EFMSGRPLGSARQGDGKSMPNQCTSNPTLADPARGRGWNGWGNGLSNTRFQTAAAAGL
                     TAAQVPRLKLKWAFGFPTGVSANAQPTVVAGRVFVGSDNGFFYSLDAKTGCVYWSFQQ
                     GSIVRNAPAVGAVMGQGSARFAAFFGDGHANVYALDAQSGRLLWKTKVDSHFVARITA
                     GVTYYNGKVFVPVSSSEEFSSGHPDYSCCTSRGSVVALDANTGKEIWKSWVVPEEPKP
                     YRTMPNGVVLYKPAGGAVWNAPTVDPARGAIYVGTGDATTAPPAKTTDAIMAIDINSG
                     KLLWSYQATENDVFMGGCNGPNRSEACPSPMGPDMDIGNSPILTTLPSGKRALLGGTK
                     SADVFALDPDNNGALLFRMNAAGGPTGGGRGGRGSIVWGGAADAEHVYYGAGAAGLAA
                     IRPTTGERAWVFQPQGRGVALGAAPTVIPGVVFQGGSNGMLYAVSAADGKQLWEFDTS
                     QDFETVNRMVAHGGAISVSGAVVVDGMVYVGSGYAVGTGATAGNVLLAFGID"
     gene            1984..>2793
                     /locus_tag="VFC01_21930"
     CDS             1984..>2793
                     /locus_tag="VFC01_21930"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HZN82318.1"
                     /translation="MVLKPTPDSNINVELWMPTSNWNGRFLAVGNGGFAGSIQGYGDM
                     QIALRRGYATAATDTGHSDADGTGGMFGLGHPEKIVDFAYRAVHEMTVKSKQVINEFY
                     DRAPRFSYFKGCSTGGRQAAMAAQRFPDDYDGIIGGALANRHIQMHTAGVARNIELAR
                     HPERTIPEDKAKLVNDTVMNACDTLKEGFLNNPRECKVDFSKLSCTGADSPTCLTAPQ
                     MRTVETFYGGLKNSKGELIFSGQALGNPLPALRGATGTPGGGYDTVRIWGFQ"
BASE COUNT          545 a          872 c          901 g          475 t
ORIGIN      
        1 agccgttgag cgcgctccct cgccgacaac gattcgcgag atgacgccgg agcggatcta
       61 cgactcactc gccaccggtt ccatgcaggc gcagagcact ggactttccg atgcgcaaaa
      121 gaaggcgctg gcggagttca tgtccggacg accgctcggc agcgcccgac aaggtgacgg
      181 taagagcatg ccgaaccagt gcacgagcaa tccgacactg gcggatccgg cgcgcggtcg
      241 tgggtggaat ggttggggca atgggctgtc gaacacgaga ttccaaacag cagccgccgc
      301 cgggctgacc gcagctcagg ttcctcgttt gaagctgaag tgggcgttcg gcttcccgac
      361 aggcgtttct gccaacgccc agccgacagt ggttgccggg cgcgtgttcg tcggaagcga
      421 taacgggttc ttctactcgc tcgacgctaa gaccggatgc gtgtactggt ccttccaaca
      481 gggatccatc gttcgcaacg cacctgcggt tggcgccgtc atggggcaag gttcggcacg
      541 cttcgccgca tttttcggcg acggtcacgc caacgtctac gcgctcgatg cgcagagcgg
      601 ccgcctgctc tggaagacga aagtcgactc gcatttcgtc gcgcgcatca ccgcgggcgt
      661 gacctactac aacggtaaag tgttcgtgcc ggtgtcatcc tccgaagaat tcagcagcgg
      721 acatcccgac tattcctgct gcacctcgcg aggcagcgtc gtcgccctcg atgcgaacac
      781 cggcaaggag atctggaagt cctgggtggt tccagaggaa cccaagcctt atcgcacgat
      841 gcccaatggt gtggtgctgt acaagcccgc gggcggtgcg gtctggaatg cacctaccgt
      901 ggatccggct cgtggcgcga tctatgtcgg aacgggtgac gccacgaccg cgccacccgc
      961 caagacgacc gacgcgatca tggcgatcga catcaacagc gggaagctgc tgtggtccta
     1021 tcaagccact gagaacgacg tcttcatggg cggttgcaac ggacctaacc ggagtgaagc
     1081 atgcccaagc ccgatggggc cggacatgga catcggcaac tcgcccatcc tgaccaccct
     1141 gcccagcggc aaacgcgcgc tcctcggcgg aacgaagagc gcggatgtct tcgccctcga
     1201 tccggataac aatggcgcac tgctgtttcg gatgaacgct gctggcggac cgactggtgg
     1261 tggtcgcggc ggtcgcgggt cgattgtctg gggcggtgcg gcggacgcgg agcatgtgta
     1321 ttacggcgcg ggcgcggcgg gactggccgc gattcgaccc accaccggcg aacgcgcatg
     1381 ggtcttccaa cctcagggtc gtggcgtcgc gctcggtgcg gcccccaccg ttattccggg
     1441 cgttgtcttc cagggtggat ccaacggaat gctgtatgcg gtctcggccg cagatggcaa
     1501 gcagctctgg gagttcgaca cctcgcagga ttttgaaacc gtgaaccgaa tggtggcgca
     1561 cggtggggcc atcagcgtgt ctggggcagt cgtcgtcgat gggatggtgt acgtcgggtc
     1621 cggctacgct gtcggcaccg gcgcgacagc cggcaacgtc ctcttggcgt ttggtatcga
     1681 ctaagtccgg aacccggaac atggaacccg gaacccggaa cgtcccgggg accgaggacc
     1741 gtggaccgga gacggaaaca aaggagatca gcgatgctcg cactgctcag cctcgcgctt
     1801 ctcggagccg cggccgcacc aaatacgtgt gagagcctga cggctgtccg gttaccgcag
     1861 gcaacgatca tgagcgcctc ggtgattccg gccgggccat tcgtccagcc tggcgcgggc
     1921 cgaggccggc agggcgctgc gcctgaaaca gtcgaaccgc tccccgagca ctgtcgcgtg
     1981 aagatggtgc tcaagccgac gcccgactcc aacatcaacg tcgagctgtg gatgccgacg
     2041 tcaaactgga acggtcgatt cctcgcggtt ggcaacggtg gcttcgccgg ttccatccag
     2101 ggatacggcg acatgcagat cgcgctgcgc cgcggctacg caacggcagc gaccgacacg
     2161 ggccattccg atgccgatgg cacaggcggg atgttcggcc ttggtcatcc tgaaaagatc
     2221 gtggacttcg cctaccgcgc cgttcacgag atgaccgtga aatcgaagca ggtcatcaac
     2281 gagttctacg atcgagcacc gcggttctcg tacttcaagg gttgctcaac cggcggccgc
     2341 caggccgcga tggcggcgca acgcttcccc gacgactacg acggcatcat cggtggtgca
     2401 ttggccaatc gacatatcca gatgcacacg gccggcgtcg cacgcaacat cgagctggcg
     2461 aggcaccctg agcgcacgat cccggaagac aaagccaagc tcgtgaacga cacggtgatg
     2521 aacgcatgcg acacgctcaa ggaaggcttc ctgaacaacc cgcgtgaatg caaagtcgac
     2581 ttctcgaagc tctcatgcac gggtgctgat tcaccgacct gcctcacagc cccgcaaatg
     2641 cgcacggtcg agaccttcta cggtgggctc aagaacagca agggcgagtt gatcttttca
     2701 ggccaggcgc tgggcaaccc actgccggca ctccgcggag caacggggac accaggcggt
     2761 ggctacgaca cggttcgcat ctggggcttc cag
//
LOCUS       DASRJA010000499         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3699441,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000499 DASRJA010000000
VERSION     DASRJA010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3699441"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VFC01_21935"
     CDS             <1..717
                     /locus_tag="VFC01_21935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HZN82319.1"
                     /translation="SAGTLRRVLAMQTDPYSVAPLWPDAVSIAGLQARFGLDAGKWTA
                     EQMAQVALDSFAVAQRTDSEKPAKSIDELLARPYFADPLRRHDIAPITDGASAVVLAA
                     GDRARELRENPAWITGFEHRIETPVLGARDLTTSPSTEASAKAATGGDAGSIEVAEIY
                     APFTHQQLILTEAIGLGASTAVNPSGGALAANPMFSAGLERIGFAAQHIFNGSAQRVL
                     AHATSGPALQQNLVAVLEGK"
     gene            720..1889
                     /locus_tag="VFC01_21940"
     CDS             720..1889
                     /locus_tag="VFC01_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HZN82320.1"
                     /translation="MAGQLAAVLGTGQTKYVAKRQDVSMNGLVREAIDKALADSGSTF
                     DDIDAVVVGKAPDFFEGVMMPELFLADAVGATGKPLIRVHTAGSVGGSTGVVAASLVQ
                     SGKYKRVLAMAWEKQSESNAMWALSIPVPFTKPVGAGAGGYFAPHVRAYIRRSGAPTH
                     IGAMVAVKDRLNGAKNPLAHLHQPDITLEKVLASQMLWDPIRFDETCPSSDGACALVI
                     GNEDIADKRVADGHPVAWIHATALRTEPLAFSGRDQVNPQAGRDAAKALWKAAGITSP
                     IDEIDAAEIYVPFSWFEPMWLENLGFAPEGEGWKLTEAGETAIGGRIPVNASGGVLSS
                     NPIGASGMIRFAEAAIQVMGKAGDHQVEGARKALGHAYGGGSQYYSMWVVGADKP"
     gene            1895..>2431
                     /locus_tag="VFC01_21945"
     CDS             1895..>2431
                     /locus_tag="VFC01_21945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN82321.1"
                     /translation="MQYTVSVAMSPVDQLVELAKTAEEVGFDSIALPDSIFYMEKQSA
                     DYPYTADGSRMWNEDTPWADPLIVAGAMGAVTTTLRFYTNVMKLGARNPLLLARQVGS
                     VANLTSNRFGFGVGIGWAPEEFEWCGVPYAKRGKRVDEMIEVIKLVLAGGMVEFHGDF
                     YDFDKLQMSPAPSKPVPFY"
BASE COUNT          424 a          801 c          831 g          375 t
ORIGIN      
        1 tcggcgggca cgctacgccg ggtgctggcc atgcagaccg acccgtacag cgttgcgcca
       61 ctgtggcccg acgcggtgtc gatcgccggc ctgcaggcac gcttcggcct tgacgccggc
      121 aagtggacgg ccgagcagat ggcccaagtg gcgctggatt cgttcgccgt tgcccagcgc
      181 accgattcgg agaaaccggc caagtccatc gacgaactac tcgcccgccc atacttcgcc
      241 gatccgctgc gccgacacga catcgccccg atcaccgacg gcgcgtccgc ggtcgtgctc
      301 gctgcgggcg accgcgcccg cgagcttcgc gaaaacccgg cctggataac cggtttcgag
      361 caccgcatcg aaaccccggt gctcggcgca cgcgatctga ccacctcacc gtcgaccgag
      421 gcatcagcca aggccgcaac gggcggtgac gccggttcca tcgaggtggc cgagatctac
      481 gcgccgttca cccatcagca gctgatcctg accgaagcga tcggccttgg ggcgtcgacg
      541 gccgttaacc cgtccggcgg cgcgctcgcg gcgaacccga tgttctcggc ggggctggag
      601 cgcatcggct ttgccgcgca gcacatcttc aacggctcag cacagcgagt gctcgcgcac
      661 gccacgagcg gtcctgcgct gcaacagaat ctcgtcgccg tcctggaagg gaaataatca
      721 tggcggggca gctggctgcg gtgctgggca ccgggcagac gaagtacgtc gccaagcggc
      781 aggacgtttc gatgaacggc ctggtgcgcg aggccatcga caaggcgctg gcggactcgg
      841 gctcgacgtt tgacgacatc gacgcggtcg tggtcggaaa ggcgccggac ttcttcgagg
      901 gcgtgatgat gcccgaactg ttcctggccg acgccgtcgg cgctaccggc aagccactaa
      961 tccgcgtgca caccgcgggt tcggtgggcg ggtccacggg tgtcgtggcc gccagcctgg
     1021 tgcagtccgg caagtacaag cgcgtgctcg cgatggcgtg ggagaagcag tcggaatcga
     1081 atgccatgtg ggcgttgagc attccggtgc cgttcaccaa acccgtcggc gccggcgccg
     1141 gcggctactt cgccccccac gtgcgggcgt acatccgacg gtccggcgcg ccgacgcaca
     1201 tcggcgcgat ggtggccgtc aaggaccggc tcaacggcgc gaagaacccg ctggcgcacc
     1261 tgcaccagcc cgatatcacc ttggagaagg tgctggcgtc ccagatgctg tgggacccga
     1321 tccgtttcga cgagacctgt ccgtcgtcgg acggcgcctg cgcgctggtg atcggcaacg
     1381 aggacatcgc ggacaagcgc gtcgccgatg gacatcccgt cgcgtggatc cacgccaccg
     1441 cgctacgcac cgaaccactc gcgttctccg gccgtgacca ggtcaacccg caggccggcc
     1501 gcgacgcggc caaggcgctg tggaaggcgg cagggatcac cagcccgatc gacgagatcg
     1561 acgccgccga gatctacgtg ccgttctcgt ggttcgagcc gatgtggctg gagaacctcg
     1621 ggtttgcgcc cgaaggcgaa ggctggaagc tgaccgaggc cggcgaaacc gcaatcggcg
     1681 ggcggatacc cgtcaacgcg tccggcggcg tgctgtcgtc caatccgatt ggggcgtcgg
     1741 gcatgatccg gttcgccgag gcggcgatcc aggtgatggg caaggcgggt gaccaccagg
     1801 tcgagggcgc acgcaaggcg ctgggccatg cctacggcgg cgggtcgcag tactactcga
     1861 tgtgggtcgt cggagcggac aaaccgtgac acgcatgcag tacacggtca gcgtggcgat
     1921 gagcccggtc gaccaactcg tcgagctcgc caaaacggcg gaggaagtcg ggttcgactc
     1981 gatcgcgctg cccgattcga tcttctacat ggagaagcag tcggccgact atccctacac
     2041 cgccgacggg tcgcgcatgt ggaacgagga caccccttgg gccgatccgc tgatcgtcgc
     2101 gggagccatg ggagcagtga ccaccacgct gcggttctac accaatgtga tgaagctggg
     2161 cgcgcgcaat ccgctgctgc tcgcgcgcca ggtgggttcc gtggccaacc tgaccagcaa
     2221 ccgattcgga ttcggcgtcg ggatcggttg ggcgccggag gaattcgaat ggtgcggcgt
     2281 cccgtacgcc aaacgcggca agcgcgtcga cgagatgatc gaggtcatca agctcgtgtt
     2341 ggccggcggc atggtcgaat tccacggcga cttctacgat ttcgacaagc tgcagatgag
     2401 cccggcgcca agcaagccgg tgcccttcta t
//
LOCUS       DASRJA010000500         5973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2288796,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000500 DASRJA010000000
VERSION     DASRJA010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2288796"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFC01_21950"
     CDS             complement(<1..325)
                     /locus_tag="VFC01_21950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HZN82322.1"
                     /translation="MTWKRPSARVRELIRQAAQVTVNAPPEWLEELDDAVLGANPAIA
                     VDPDLASAVSRSNLANLLFWATANVRDPGAPVPPNAGPEPMNVGRELVRRGLDAYALD
                     AYRVGE"
     gene            457..2040
                     /locus_tag="VFC01_21955"
     CDS             457..2040
                     /locus_tag="VFC01_21955"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN82323.1"
                     /translation="MAHTATHIEHVDVLIVGAGISGIGAAYYLQKEHPGRSYAILEAR
                     GASGGTWDLFRYPGIRSDSDLHTFGYEFKPWRDEDAIASADKILTYLRETAAENSIDA
                     NIRFHHKVLAAAWSSDDGRWLVDVERFGDTEDTEELVQFSANWIFCAGGYYRYDQGYT
                     PEFPERDRFLGQIVHPQHWPEDLDYSGKKVVIIGSGATAVTLVPAMAGTASHVTMLQR
                     SPTYVLPVPSKDTFANAAQMLLGAKRGYALARRKNIIRQRAVYQLSQKYPGAARRLIR
                     YLNAKELPEGYPVDEHFNPAYNPWDQRLCAVPDADLFKAISGGTASVVTDRIANFTET
                     GIQLASGTHLDADIIVTATGLNIQLFGGVSLTVDGAPVDPADSVVYKAMMLAGVPNFA
                     FAFGYTNSSWTLKVGLLCEHFCRLLSHMDANGYDIARPELGDPGMPTQPMLDLGAGYV
                     QRALNRLPKQGEGDPWRVQMDYYHDVEQLRQGSVANPHLHFGSSNQAAGEASRIPAGS
                     TAVGSDRVAAAAGPSTSPR"
     gene            complement(1958..2798)
                     /locus_tag="VFC01_21960"
                     /pseudo
     CDS             complement(1958..2798)
                     /locus_tag="VFC01_21960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(2795..3313)
                     /locus_tag="VFC01_21965"
     CDS             complement(2795..3313)
                     /locus_tag="VFC01_21965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HZN82324.1"
                     /translation="MTRYAAFLRGVNVGGVNLKMAEVAKAFEDAGFTNVRTILASGNV
                     LLESRSGVAAVRKKAEAALRDAFGYDAWVLAYDIKTVAAISKAYPFEREVEGRHSYIT
                     FVTDEKVLDELAALGKDAGPDEKVKRGKGVIYWQAPKGTLGTTIGKTMGMKRYKSSTT
                     TRNLRTVDKVLK"
     gene            3447..4625
                     /locus_tag="VFC01_21970"
     CDS             3447..4625
                     /locus_tag="VFC01_21970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN82325.1"
                     /translation="MVELIESNSRKTDTFRVGVIENIVNSRYGPDPLLRAGHLAAVAA
                     GVDSFWVPDHLNSLFPRSICTQKYMGAARLAPSPDAYLEPWTMLGYLAAHNRLGRLRL
                     GVCVTDTARRNPAVTAQAAATLQLLTRGRAILGIGTGEREGNEPYGVDWSKPVARFEE
                     ALATIRALWGSEGEPVNRDSAYFPLRNAVFDLPPYKRRWPQIWIAAHRPRMLRITGRQ
                     ADAWFPAFIFRPKDYARCLEVVQSAASDAGRDPSAVIPAALLAVVTGRTADDVDEALE
                     SEGAKAYALTGSAEVWARHGVEHPMGADFTGAQDIQPQTLDEQTVLTYTAKVPRSLLT
                     EMYLTGTAEQVIEQVAEWRAHGLRYIIVANSSGLQRSLRKGMASSMPFGKIMRGLKKL
                     "
     gene            4655..5098
                     /locus_tag="VFC01_21975"
     CDS             4655..5098
                     /locus_tag="VFC01_21975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZN82326.1"
                     /translation="MGRQVFDDKLLALLCGNSLGVLATIKRDGRPQLSNVSYWFDKPN
                     LAIQASITESRAKTRNLRRDPRAAIHVSSDDGWAYAVAEGDATLTPPAAAPDDDTVEA
                     LIALYRNIAGEHPDWDDYRRAMVEDRRVVLTLPISHVYGMPPGRR"
     gene            5140..5307
                     /locus_tag="VFC01_21980"
     CDS             5140..5307
                     /locus_tag="VFC01_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 domain-containing protein"
                     /protein_id="HZN82327.1"
                     /translation="MADAPEDDTKRKFREALERKKAKAAGGADHKDGGAKQPRAHGPL
                     ESRREFRRKSG"
     gene            5311..5922
                     /locus_tag="VFC01_21985"
     CDS             5311..5922
                     /locus_tag="VFC01_21985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HZN82328.1"
                     /translation="MTPEFLITTLIVVATPGAGVLYTVAAGISRGWRASLVAAFACTL
                     GIVPHMIAAVTGLAAIMHASAMAFSIIKLLGVAYLLYLAWKTFRDKSIFEVQAGPAKV
                     SPWRVIVSGVLVNTLNPKLTIFFFAFLPQFVAPDRPGAVLLMVWLSAIFMAVTLAVFS
                     VYGVFAAAVRREVISRPRIVAWMRRSFAATYVVLAGRLAAQTR"
BASE COUNT         1023 a         1961 c         1961 g         1028 t
ORIGIN      
        1 cttcgccgac gcgatacgca tccagcgcgt acgcgtcgag tccccggcgg accagttccc
       61 gcccgacgtt catcggctcc ggcccggcgt tcggcggcac tggcgccccg ggatcgcgga
      121 cgttggcggt ggcccagaac agcaggttgg ccaggttgct gcggctcacc gcggaggcga
      181 ggtccgggtc gaccgcgatc gccgggttgg cgccgagtac ggcatcgtcg agctcttcga
      241 gccattccgg aggcgcattg acggtgacct gcgcagcctg ccgaatcagc tccctgaccc
      301 gcgccgatgg gcgtttccac gtcatcacac cacggtagac cgtctggtgc aaagtgcacc
      361 agatcgcccc gatgttgaga cgatttgctc ttgggtgaac ctcgcctcaa cgggaccatc
      421 aatacaacga aaaacccatc gtcaggagtc actcccatgg cccacaccgc cacccacatc
      481 gagcacgtcg acgtcttgat cgtcggcgca ggaatctccg gcatcggcgc ggcttactac
      541 ttgcagaagg agcaccctgg ccggtcctac gcgatcctcg aagcccgcgg cgcgtccggt
      601 ggcacgtggg acctgttccg ctatccgggg attcgctcgg attccgatct gcataccttc
      661 ggctacgagt tcaagccgtg gcgcgacgag gacgccatcg cgtcggcgga caagatcctg
      721 acctacctgc gcgagactgc cgcggagaac agcatcgacg ccaacatccg gttccaccac
      781 aaggttctgg ccgccgcttg gtccagcgac gacgggcgct ggctggtcga tgtcgagcgc
      841 ttcggcgaca ccgaggacac cgaggaactg gtgcagttca gcgccaactg gatcttctgc
      901 gccggcggct actaccgcta cgaccagggc tacacgccgg agttccccga gcgcgaccga
      961 tttctcggtc agatcgtgca cccacagcat tggcccgagg acctcgacta cagcggcaag
     1021 aaggtcgtca tcatcggcag cggcgcgaca gcggtcaccc tggtgcccgc gatggcaggc
     1081 accgcgagtc acgtgacgat gctgcagcgc tcaccgacct atgtgctgcc ggtgccgtcg
     1141 aaagacacct tcgccaacgc agcgcagatg ctgctgggcg ccaagcgcgg ctatgcgctc
     1201 gcccgccgca agaacatcat caggcagcgc gccgtctacc agttgtccca gaagtatccg
     1261 ggcgcggcgc ggcggctgat ccgctacctc aacgccaagg agctacccga gggatatccg
     1321 gtcgacgagc acttcaaccc cgcctacaac ccgtgggatc agcggctgtg cgcggtgccc
     1381 gacgccgacc tgttcaaggc gattagcggc gggaccgcct cggtggtcac ggatcggatc
     1441 gccaacttca ccgaaaccgg catccaactg gcatccggca cgcatctcga tgccgacatc
     1501 atcgtcaccg caacaggttt gaacattcag ctgttcggtg gcgtgtcgct gacggtcgac
     1561 ggcgcgcccg ttgaccccgc cgactccgtc gtctacaagg ccatgatgct ggccggcgtg
     1621 ccgaacttcg cgttcgcgtt cggctatacc aactcgtcgt ggacgctgaa ggtgggcctg
     1681 ctgtgcgagc acttctgccg cctgctgagc cacatggacg ccaacggcta cgacattgcg
     1741 cgtcccgagc tgggcgaccc cgggatgccg acccagccga tgctcgacct cggcgccggg
     1801 tatgtccagc gcgccctcaa ccggttgccg aagcagggtg agggcgaccc ctggcgcgtg
     1861 cagatggact actaccacga tgtcgagcag ctgcgtcagg gttcagttgc caacccccac
     1921 ttgcactttg ggtcctcgaa tcaggccgct ggcgaggcta gccgaattcc agcaggatca
     1981 acagcggtcg gatcggatcg agtagcggca gccgctggac cgtccacatc accgcgttga
     2041 acacattgcc gcgacccgcg gcatgaggca gatcgtggac ggccctgatg ccgggcacgg
     2101 tgctgacgag gtttgccgca tcggacgctg acaggctgaa cggcatcgga gggatgcggt
     2161 aacgcaagga tgtccgcatg ccgcgacgcg acaatgcggc gaagcccgac ggcggcaggt
     2221 cgaacatcat ctgcccgccg ggaaaccggg cggcgcattc ggcgatcagc cccagtgcct
     2281 cgtcggcctc caggtacatc atcagtccct cagccgtgat gaacacgccg tgctcggtct
     2341 cgacttggtc catccagctg acgtcgagtg ccgactgggc gcacatccgc acccgctccg
     2401 agcccggcag gagtttgcgg cgtagctcga ccattggcgg cagatcgact gtgagccact
     2461 ggaattggtc acccaggccc gcggcgtcga gacggtagaa gctggtcctg cagcccctcc
     2521 gccagcgcca ccacggtggc cttgggatgg tcgaggaggt agcggcgcgt ctgccggtca
     2581 tagcccaacg cgcggacagc catgtcctgg cggcgcgaga agccgaactt cgcgaagtcg
     2641 tagtcgatgg aatcggccag ctcgatcgcc atcgggtcct cgatgatcga atccggcctg
     2701 cgcgcctcgg tcgcccggac ccgcagggtc atcagcgcgg tctcggacac accggtcagc
     2761 acggtgccat cggccttcgc cgtcggcgtg gagctcattt cagaaccttg tcgaccgtgc
     2821 gcaggttgcg ggtggtcgtc gaggacttgt agcgcttcat acccatggtc ttcccgatgg
     2881 tcgtgcccag cgtgcctttg ggagcctgcc agtagatgac acctttaccg cgtttgacct
     2941 tctcgtcggg cccggcgtcc ttgccaagcg cggcgagttc gtcgagcacc ttctcgtcgg
     3001 tgacgaatgt gatgtaggag tgccggccct cgacctcgcg ctcgaacgga tacgccttgg
     3061 aaatcgctgc cacggttttg atgtcgtagg caagaaccca ggcgtcgtaa ccgaacgcgt
     3121 cacgcaacgc cgcttcggct ttctttcgga ccgcggccac cccggaccgg ctctccagca
     3181 gaacgttgcc gctggccaaa attgttcgca cgttggtgaa tccggcgtct tcgaacgcct
     3241 tggccacctc ggccatcttg aggttgacgc cgccgacgtt gacgccgcgc aggaaggccg
     3301 cgtatcgggt catgtcgtca ttttagaggc cgacgccgat cgaaatgtgc tgtgtttgct
     3361 tgccaagtca tgcagcagct aacggccaga ccggattcgc gatgcgccag cggtatcggt
     3421 gaaacaatcc atcgctgggg gctgcgatgg tcgagctaat tgaatcgaat tcgcgaaaga
     3481 cggacacttt ccgggtcggg gtaatcgaga acatcgtcaa ttcacggtat gggcccgacc
     3541 cgctgctgcg cgcgggacac ctggccgccg tcgcggcagg cgtcgactcg ttctgggtgc
     3601 ctgatcacct caattcgctt ttcccgcggt cgatctgcac gcagaagtac atgggggccg
     3661 ccagattggc gccctcgccc gatgcctatc tcgagccgtg gaccatgctc gggtatctcg
     3721 ccgcgcacaa ccgcctcggg cgtctccggc tcggggtgtg tgtcaccgat acggcgcggc
     3781 gcaaccccgc ggttacagcg caagccgcgg cgacgctgca gctgctgacc cgtggacgag
     3841 ccattctggg catcgggacc ggcgagcgtg agggcaacga accgtacggg gtcgattggt
     3901 cgaagccggt ggcgcggttc gaagaagccc tcgcgaccat ccgggcgctg tggggctccg
     3961 agggtgaacc cgtcaacagg gactcggcgt attttccgtt gcgcaacgcc gtgttcgacc
     4021 ttccaccgta caagcgccgc tggccgcaga tctggatcgc cgcgcaccgg ccacgcatgc
     4081 tgcgcatcac cgggcgccaa gcggatgcgt ggtttcccgc gttcatcttc aggccaaagg
     4141 attacgcgcg ctgtctggaa gtcgtgcagt cggctgcctc cgacgccggc cgcgacccgt
     4201 cggcggtgat ccccgcggcg ctgttggctg tcgtcaccgg ccgcactgcg gacgacgtcg
     4261 acgaagcgct ggagtccgag ggcgccaagg cgtacgcctt gaccggatcg gccgaggtgt
     4321 gggcgcgcca cggcgtcgag catccgatgg gcgccgattt cactggcgcg caagatatcc
     4381 agccgcaaac cctcgatgag cagacggtgc tcacgtacac ggcgaaggtg ccgcggtcac
     4441 tgctcacgga gatgtacttg accggaacag cggagcaggt gatcgagcag gtggccgaat
     4501 ggcgcgccca cggcctgcgc tacatcatcg ttgcgaattc aagcggcttg cagcgcagtc
     4561 tgcgcaaggg gatggcgtcg tcgatgccat tcgggaagat catgcgcggc ttgaagaagc
     4621 tatgaccgtt gtcgatgaga cgtaggctgc cgatatggga cgccaagtat tcgacgacaa
     4681 gctgttggcc ctgctctgcg gaaactcact cggcgtgctg gccaccatca aacgcgacgg
     4741 caggccgcag ttgtcgaatg tgtcctactg gttcgacaag cccaatctgg cgatccaggc
     4801 ctcgatcacc gagtcgcggg ccaagacccg caacctgcgc cgcgatccgc gagcggcgat
     4861 acatgtgagc tccgacgacg gctgggccta cgccgtcgct gagggcgacg cgaccctcac
     4921 gccgcccgcc gccgctccgg acgacgacac cgtcgaggcc ctgattgcgt tgtatcgcaa
     4981 tatcgccggt gagcacccag attgggacga ctatcggcgc gcgatggtcg aggaccgccg
     5041 ggtggtgctg acgctgccga tctcgcacgt gtacgggatg ccgccaggca ggcggtaaag
     5101 ggcacgacgg ccgcgggttg gccgcgctag gctgcggtca tggctgatgc accggaagac
     5161 gacaccaaac gcaagttccg ggaagcgctg gagcggaaaa aggccaaggc agcgggcggc
     5221 gccgaccaca aggatggcgg agccaagcag ccgcgggcgc acgggcctct cgagagtcgc
     5281 cgggagttcc gccgcaagag cggttagccc gtgacgccag aattcctgat caccacgctc
     5341 atcgtggttg cgacgccggg tgccggcgtg ctgtacacgg tcgcggccgg gatttcgcgc
     5401 gggtggcggg ccagcctcgt ggcggcgttc gcctgcacgc tcgggatcgt gccgcacatg
     5461 atcgccgcgg tgacgggcct ggccgccatc atgcacgcca gcgcgatggc gttctccatc
     5521 atcaagctgc tgggcgtcgc ataccttctt tacttggcgt ggaagacatt tcgcgacaaa
     5581 tcgatcttcg aggtgcaggc cggtccggcg aaggtgtcgc cgtggcgggt gatcgtgtcg
     5641 ggcgttctgg tcaacacatt gaacccgaag ctgaccatct tcttcttcgc gttcctgccg
     5701 cagttcgtcg cacccgatcg ccccggcgcg gtgttgctga tggtgtggct gagcgcgatc
     5761 ttcatggcgg tgacgctggc ggtgttcagc gtctacggcg tgttcgccgc ggcggtccga
     5821 cgcgaggtga tctcccggcc acgcatcgtc gcgtggatgc ggcggagctt cgccgcgacc
     5881 tacgtcgtgc tggccggcag gctcgcggcg cagacgcgct gagggtcgct ggccaggctg
     5941 cgggcggcga ccagcgcgat cagcgtgacg gcg
//
LOCUS       DASRJA010000501         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2449136,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000501 DASRJA010000000
VERSION     DASRJA010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2449136"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..2057
                     /locus_tag="VFC01_21990"
     CDS             294..2057
                     /locus_tag="VFC01_21990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN82329.1"
                     /translation="MDAIGEVNAREQHGDAESLLFNPHTYDPSHFDDVTRRLLRATID
                     WFEHLGKETLLQNYRECVWYADFLNFVAKEHLFAKMGTPAREANGDRDKRWDTSRISA
                     FSEILAFYGVDYWYAWQVTILGLGPVWQSDNAWARRRAAQWLDDGAVFAFGLSEREHG
                     ADIYTTDMVLTPDGGGGFRATGGKYYIGNANVARMVSVFGRRSDVDGPDGYVFFAADS
                     QHENYQLVKNIVQAQMFVAEFRLENYPVRPQDVLHTGQAAFAAALNTVNVGKFNIGPA
                     SIGMCEHAMYEAVTHAHNRILYGNRVTDFPHVRRSFVDAYARLIAMKAYGDRAIDYFR
                     TASPRDRRYLLFNPVSKMKITTEGEKVIELLWNVIAAKGFEPDTYFRGAGKDVGALAK
                     LEGTVHVNLALILKFMPNYLFAPTEQPVVPTRQDPVDDEFLFRQGPARGLGEVTFAAW
                     RLAFEPFAELPNVARFRKQAEGFTTLLRTAAPDAEQQQDLDFRLVLGQLFTLIVYAQL
                     ILEQAELIGLDPDVLDTIFEVFVRDFSALAVELHGKASSTEAQQQWALANVRKPVIDT
                     GRFARVWEQVEALSGAYLMSP"
BASE COUNT          454 a          765 c          806 g          436 t
ORIGIN      
        1 taaagcgtcc aggcagcccg actaatcgtc tgtggctcgg tagcgggcgg tgagccgcga
       61 gtcctgcagg gcggccaccc cgcagttgag cgctcgcttc tctcaagcat ccgctcgaag
      121 gaacttctgc ggggatctcg aaggaacttc tgcggggata gagaacgagg atgatctcag
      181 ctcatgctgc gctgacacct gcagcgggac cgctcgggcg cggcatccct gaatggctgc
      241 gacgaccgcg ggcgcgtagc gtcaaggttc gtcacccgtg caaggagacg aagatggacg
      301 ctataggcga ggtcaacgcg agagaacaac acggcgacgc cgagtcgctt ctgttcaacc
      361 cgcacaccta cgacccgtcc catttcgacg acgtcaccag aaggctgctc agggcgacca
      421 tcgactggtt cgagcacctg ggcaaggaaa cgcttctgca gaactaccgc gagtgtgtct
      481 ggtacgcaga cttcctgaac tttgtcgcca aggagcattt gttcgcgaag atgggaacgc
      541 cggcgcgcga ggccaacggc gaccgcgaca aacgctggga caccagcagg atctcggcgt
      601 tcagcgagat cctggccttc tacggcgtgg actattggta cgcctggcag gtcaccatcc
      661 tcggcttggg gccggtctgg cagagcgaca acgcctgggc tcgcaggcgc gcggcccagt
      721 ggctcgacga tggtgcggtg ttcgcgttcg ggctgtcgga gcgcgaacac ggggccgaca
      781 tctacaccac cgatatggtg ttgaccccgg atggaggggg cggtttccga gccacgggcg
      841 gcaagtacta catcggtaac gccaacgtgg cccgcatggt ctcggtgttc ggccggcggt
      901 cggacgtgga tggcccggac gggtatgtgt tcttcgcggc ggacagccag cacgagaact
      961 accagctggt caagaacatc gtccaagcgc agatgttcgt ggccgagttc cggctagaga
     1021 actacccggt acgcccgcag gatgtgctgc acaccgggca agcggccttc gctgccgcgt
     1081 tgaacacggt gaacgtcggc aagttcaaca tcgggcccgc ctccatcggc atgtgcgagc
     1141 atgccatgta cgaggcggtc acccatgccc acaaccgcat cctctacggc aaccgcgtga
     1201 ctgactttcc gcacgtgcgc cggtcctttg tggatgcata cgctcggctg atcgcgatga
     1261 aggcctacgg tgaccgcgcg atcgactact tccggacggc cagcccgcgg gatcggcggt
     1321 acttgctgtt caacccggtc agcaaaatga agatcaccac cgaaggcgag aaagtcatcg
     1381 agctgctgtg gaacgtcatc gccgccaagg gattcgagcc cgacacgtac ttccgcggcg
     1441 ccggcaagga tgtcggcgcg ctggccaagc tcgagggcac ggtgcacgtc aatctcgcgc
     1501 tgatcttgaa gttcatgccg aactacctgt tcgcgccgac tgagcaacca gtggtgccta
     1561 cccgacagga cccggttgac gacgagttcc tgttccgcca gggcccggcg cgcggcctgg
     1621 gcgaggtcac gttcgccgct tggcgactgg ccttcgagcc gttcgccgag ctgcccaacg
     1681 tcgcacggtt ccggaagcag gcggagggct tcacgaccct gctacggacc gcggcgccgg
     1741 acgccgagca acagcaggac ctagacttcc ggctggtgct cggacagctg ttcacactga
     1801 tcgtctatgc gcagctgatc ctcgagcagg ccgaactgat tgggcttgac cccgacgtac
     1861 tcgatacgat cttcgaggtc tttgtacggg atttctcggc gctcgcggtc gagctgcatg
     1921 gcaaggcgtc atcaaccgag gcccaacagc agtgggcact ggccaacgtg cgcaaaccgg
     1981 tcatcgacac cggccggttc gcgcgggtgt gggagcaggt cgaggcgctc tccggcgcct
     2041 atctgatgag cccgtgacca tggctcgagg tgtggagcgg tcaccgtggg cctagaccgg
     2101 cgcttctctg gaatcgtgca gctggttgcg tacctcctga ggatcgcggt cctggttcta
     2161 tgaggcggtc ggcgcggggg cggcgatggc gcccaagcgt cccggtcctt cctcggcgta
     2221 cctcggcgcg tataaggaca aggccggcaa agtggctgga cggcgtacgt ctatcggctg
     2281 cgcgttcggc cccatccacc gatcaagctg tcctggtccg tcacgatcta tgacgccgac
     2341 actcgcgcat tgatcctgaa cgatcagaaa gttgccaatc gcttgtcgcg catggatctg
     2401 cagaaaaacg aggacggctc ggtggatatc tattgcgggc cgcaggcgcc ggctggcttc
     2461 g
//
LOCUS       DASRJA010000502         4297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3442822,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000502 DASRJA010000000
VERSION     DASRJA010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3442822"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VFC01_21995"
     CDS             complement(<1..143)
                     /locus_tag="VFC01_21995"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216112.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HZN82330.1"
                     /translation="MELTADELTEARRVIARALEEDLRYGPDVTTLATVPADATTTAS
                     MVTR"
     gene            complement(143..1681)
                     /locus_tag="VFC01_22000"
     CDS             complement(143..1681)
                     /locus_tag="VFC01_22000"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HZN82331.1"
                     /translation="MTAASGGRGFWQQRADVVVIGTGVAGLVSALAAERRGRKVMVLS
                     KIGDTATFYAQGGIAVVLPDTDDSIDAHVADTLAAGAGLCDPDAVRSIVADGYRAVTE
                     LVDDGARFDETVPGRWALTREGGHSRHRIIHAGGDATGAEVQRALDHAAAMLDIRRHH
                     VALELLHDGGAVTGVLVLNEDGLGIVHAPSVILATGGLGHLYAATTNPEGSTGDGVAL
                     AKWAGLPVSDLEFVQFHPTMLFDGHAGGRRPLITEAVRGEGAILVDAQGNSVTEGVHP
                     MGDLAPRDVVAAAIDARMKETGDPCVYLDARRIDGFAQRFPTVTAACRGAGIDPTRQP
                     IPVVPGAHYSCGGVVTDVFGRTDMAGLFAAGEVARTGMHGANRLASNSLLEGLVVGGR
                     AGKAAAAHAVDSAPTRATMPEPEVRRALPRAELQRAMTRYASVVRDGTGLQLLAKELD
                     DATPRGLRSRADFEDVALTTTAVAVAAAALARTESRGCHHRSDCPDSDPALAHSLVPA
                     AACC"
     gene            complement(1678..2721)
                     /gene="nadA"
                     /locus_tag="VFC01_22005"
     CDS             complement(1678..2721)
                     /gene="nadA"
                     /locus_tag="VFC01_22005"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZN82332.1"
                     /translation="MTVLDRMPAGDLVNRITEGPTGFTGVDGDEEWAAEVRRLAKARN
                     ATILAHNYQVPAIQDIADHVGDSLALSRIAAQAPEDTIVFCGVHFMAETAKILSPDKT
                     VLIPDQRAGCSLADSITADELRAWKAEYPGAVVVSYVNTTAAVKAETDICCTSSNAVE
                     VVASIPDDREVLFCPDQFLGAHVRRVTGRKNLHVWAGECHVHAGINGDELADQARTHP
                     DAELYVHPECGCATSALYLAGEGAFPEDRVKILSTGGMLDAARETRARQVLVATEVGM
                     LHQLRKAAPEVDFRAVNDRASCKFMKMITPAALLRCLVEGADEVDVDPETADRARASV
                     QRMIEIGQPGGGE"
     gene            2770..3462
                     /locus_tag="VFC01_22010"
     CDS             2770..3462
                     /locus_tag="VFC01_22010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZN82333.1"
                     /translation="MPHTSTAHEVLAVVFQVRGVDSRKPELHVLLWQRAREPEREKWS
                     LPGGRLGDDEDLTSSVRRQLAEKVDLRELAHLEQLAVFSDPNRVPGVRTIASTFLGLV
                     PSPATPELPPDTRWHPVSQLPPMAFDHGPMVEHAPTRLAAKMSYTNIGFALAPREFVL
                     STLRDIYSATLGYQVDATNLQRVLERRNVITRTGTTARSGRSGGRPAALYRFTDSRYR
                     VTDEFAALRPPG"
     gene            3507..>4297
                     /locus_tag="VFC01_22015"
     CDS             3507..>4297
                     /locus_tag="VFC01_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HZN82334.1"
                     /translation="MDVGSAAPVTGAEWIGQPQHEELLRGVRPLLPADDPFYDPPVGF
                     QHSRPGTVLRSRDVELAFLGVIPQRITATQLLYRTTDMHGNPDAAATTVIVPAERAPK
                     AVTPVISYQCAIDAVTSRCFPSYALRRRAVAPGAVAQFEFVLVAAALAEGWAVSVPDH
                     EGREGMWGAPYEPGYHVLDGLRAALNTERLGLAPSAPVGLWGYSGGGLASAWAAELAA
                     SYAPELNVVGAVLGSPVGDLGNTFRRLNGTYMSGLPALVVAALAD"
BASE COUNT          656 a         1498 c         1428 g          715 t
ORIGIN      
        1 cgggtgacca tcgacgcggt cgtcgtcgca tctgccggca cggtggccaa ggtggtgacg
       61 tcgggcccgt accgcagatc ctcttcgagg gcacgggcga tcactctgcg agcctcggtc
      121 agctcgtcgg cggtcagctc catcagcagc acgccgcggc gggcacaagg ctatgcgcca
      181 acgccgggtc ggagtccggg caatcggaac ggtgatgaca gccgcgcgat tcggtgcggg
      241 ccagcgcagc ggccgctacg gcgaccgcgg tcgtcgtcaa cgcgacgtcc tcgaaatccg
      301 cgcgcgaacg cagaccacga ggagtcgcgt cgtccagctc cttggccagc agctgcaggc
      361 ccgtgccgtc gcgcaccacc gacgcgtagc gcgtcatcgc ccgctgcagt tcggctcggg
      421 gcagtgcgcg ccgcacctcc ggctcgggca ttgtcgcgcg ggtaggtgcg gaatccacgg
      481 catgcgcggc cgccgcctta cctgccctgc ctccgacgac gaggccctcg agcaggctgt
      541 tggacgccag ccggttggcg ccgtgcatgc cggtgcgcgc cacctcgccg gccgcaaaca
      601 ggccggccat gtcggtccgt ccgaatacgt cggtgacgac gccgccgcag ctgtagtgcg
      661 cgcccggcac cacgggaatg ggttggcgcg tggggtcgat acccgcgcct cggcacgccg
      721 cggtgacggt cggaaagcgc tgggcgaagc cgtcgatgcg gcgcgcatcg aggtacacgc
      781 acgggtcccc ggtttccttc atccgggcgt caatggctgc agccaccacg tcgcgcggcg
      841 cgaggtcacc catcgggtgc acgccttcgg tcaccgaatt gccttgtgca tcaaccagta
      901 tcgcaccctc gccgcgcacc gcctcggtga tcagcggacg ccggccaccg gcgtgcccgt
      961 cgaacaacat cgtcggatgg aactggacga actcgagatc gctgaccggc agcccggccc
     1021 acttcgccag cgcgacgccg tcgccggtcg acccttccgg gttcgtggtg gccgcataga
     1081 gatggcccag accacccgtc gccaaaatga ccgacggcgc atgcacgatg ccaaggccgt
     1141 cttcgttgag caccaggacg ccggtcaccg ccccaccgtc gtggagcaat tcgagcgcaa
     1201 catggtggcg ccgaatatca agcatcgcag cggcgtgatc gagtgcccgc tgcacttcgg
     1261 cgcccgtcgc gtcgccgccg gcgtggatga tcctgtgccg cgagtggccg ccttcgcggg
     1321 tcagcgccca gcggcccggc accgtttcgt cgaaccgggc tccgtcgtcg accaattcgg
     1381 tgacggcacg gtagccgtcg gccacgatcg agcggaccgc gtcgggatcg cataacccgg
     1441 cacccgcggc gagcgtgtcg gcgacgtgcg cgtcgatgga gtcatcggtg tccggcagca
     1501 ccaccgcgat gccgccctgc gcatagaacg tcgcggtatc gccgatcttg ctcagcacca
     1561 tcacttttcg gccgcggcgt tcggccgcca atgccgacac caggcctgcg actccggtgc
     1621 cgatgacgac gacgtcggcg cgctgttgcc agaacccgcg gcccccgctt gcggcggtca
     1681 ttcgccaccg ccaggttgtc cgatctcgat catgcgctgg acgctggcgc gggcacggtc
     1741 ggcggtttcg ggatcgacgt cgacttcgtc ggcgccttcg accaggcagc gcagcagtgc
     1801 ggccggcgtg atcatcttca tgaacttgca cgacgcacgg tcgttgacgg cgcggaagtc
     1861 gacttccggt gcggccttgc gcaattggtg cagcatgccg acctcggtgg cgacgagcac
     1921 ctgtcgcgcg cgtgtctcgc gggccgcatc cagcatgccg ccggtggaca ggatcttgac
     1981 tcggtcctcg gggaatgccc cctcgccggc taagtacaga gccgaagtcg cgcaaccaca
     2041 ttcgggatgc acatagagct ctgcgtcggg atgcgtgcga gcctgatcgg caagctcgtc
     2101 accgttgatg ccagcgtgca cgtggcattc gcccgcccac acatgcaggt tcttgcgtcc
     2161 ggtcacccgc cggacatggg cgccgaggaa ctggtcgggg cagaacagca cctcgcggtc
     2221 gtccgggatg gacgccacca cctcgaccgc gttcgacgac gtgcagcaga tgtcggtttc
     2281 ggccttcacc gcggccgtgg tgttgacgta ggacacgacc accgcgccgg gatattcggc
     2341 cttccaggcg cgcagttcgt cggcggtgat cgagtcggcc agagagcagc cggcgcgctg
     2401 gtcgggaatc agcacggtct tatcggggct gaggatcttc gccgtctccg ccatgaaatg
     2461 cacgccgcag aacacgatgg tgtcctcggg agcctgggcg gcgatccggg acagcgcgag
     2521 tgagtcgccg acgtggtcgg caatgtcctg gatggccggt acctggtagt tgtgcgccag
     2581 gatcgtcgcg ttgcgggcct tggccagacg acggacctcg gcggcccatt cctcgtcgcc
     2641 atcgacgccg gtgaagccgg ttggaccttc ggtgattcgg ttcaccagat cgcctgcggg
     2701 catacggtcc agaaccgtca tggccgtctc ctcaatcccg tcggaggttt tcgacttata
     2761 atcgaaaaca tgccacatac tagcaccgct cacgaggtgc tcgccgtcgt cttccaggtt
     2821 cgaggcgtcg actccaggaa acccgagctt cacgtgctgt tatggcagcg tgcacgcgag
     2881 ccggagcgcg aaaagtggtc actgccgggc ggtcggctgg gcgacgacga ggatctgacc
     2941 agctccgttc gccgccaact cgccgagaaa gtcgacctac gtgaacttgc tcacctcgag
     3001 cagctggctg tgttctccga tccgaaccgc gtccccggcg tccgcacgat cgcgtcgacg
     3061 ttcctcggcc tggtgccctc ccccgcgaca cccgaactgc cgccggacac ccgctggcac
     3121 ccggtgagtc aactgccgcc gatggctttc gaccacggtc cgatggtcga gcacgccccc
     3181 actcgcctgg ccgcgaagat gtcctatacg aacatcggat tcgctttggc tccaagggaa
     3241 ttcgtgctgt ccacgctgcg cgacatctac agcgccacgc tgggttatca ggtcgacgcc
     3301 accaacctgc agcgggtatt ggagcggcgc aatgtcatca cccgcaccgg caccaccgcg
     3361 cgctcaggac gcagcggtgg gcgtccggcc gcgctgtacc gctttaccga ctcgcggtac
     3421 cgggtgaccg acgaattcgc agcattgcgc cctcctgggt gagtcgggct ggattcttaa
     3481 tgccctctta aggtaagaat gtccggatgg acgtgggcag cgcggctccg gtaaccggcg
     3541 ccgagtggat agggcagccg cagcacgaag aactgctgcg gggcgtacgt ccgctgcttc
     3601 ccgcggatga cccgttctac gacccacctg tgggtttcca gcactctcgg cccggcacgg
     3661 tgctccgcag ccgcgacgtt gagctggcgt ttctcggggt gatcccgcag cgcatcaccg
     3721 cgacccagct gctgtaccga accaccgaca tgcatggcaa cccggatgcc gccgccacca
     3781 cggtcatcgt gccggccgag cgcgcgccaa aggccgtcac ccccgtgatc tcctaccagt
     3841 gcgcgatcga cgccgtgacg tcgcgttgtt tcccgtcgta tgccctgcgc cgacgcgcgg
     3901 ttgcgcccgg tgccgtcgcg cagttcgagt tcgtgctggt tgccgcggcc ttggcggagg
     3961 gctgggccgt atcggtgcct gaccacgagg gccgcgaagg catgtggggc gcgccatatg
     4021 agcccggtta ccacgttctg gacgggctgc gcgccgcgct gaacacagaa cggcttgggc
     4081 tggcgccgtc ggcgccggtc ggcctgtggg gctactccgg tggcggcctg gcaagcgcat
     4141 gggccgccga gctggccgcc tcctacgcgc cggagctcaa cgtcgtcggc gccgtgctcg
     4201 gatcgcccgt tggtgacctg ggcaatactt tccgccggct caacggcacc tacatgtccg
     4261 gcctgcccgc gctcgtggtc gccgcgctcg ccgacat
//
LOCUS       DASRJA010000503        16312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2954728,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000503 DASRJA010000000
VERSION     DASRJA010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16312
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2954728"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFC01_22020"
     CDS             complement(<1..234)
                     /locus_tag="VFC01_22020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN82335.1"
                     /translation="MLTRLAAFVVRRPKVVLLAVVALLGFSIVFGGTVSEKLGVGGFV
                     DPAAESTQVADFLDETFSTTPNLVLQVVAHDGTV"
     gene            complement(228..908)
                     /locus_tag="VFC01_22025"
     CDS             complement(228..908)
                     /locus_tag="VFC01_22025"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN82336.1"
                     /translation="MRLFCFHHAGGGRAMFNAWNRALGPRIEVVPVEVENRERFATLS
                     QLVELLHDQLRSELDGAHVFFGHSFGALLAYRLVCLRAAAGANLPRALVVSSYAPPHL
                     PPPIPPVEDDLQLAALLSDLGGMPPELAQWPALHDRAVAATRVDLRLCASDEDADVVP
                     LPCPIHAFGGSDDPLVSEPDLHEWRSRTTEEFSVQILRGGHFYLGDGPQLLATLRRQL
                     AKIGGAQC"
     gene            complement(913..5106)
                     /locus_tag="VFC01_22030"
     CDS             complement(913..5106)
                     /locus_tag="VFC01_22030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007300675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN82337.1"
                     /translation="MADVAVVGIDCRFPKAGDPTALWKLLLEGADGIDEIPAERWNAA
                     ELHDDGTLNHRTGGLISDADAFDNDFFGITPREAEAMDPQQRLLLQTTWRAMENATLD
                     PRGQAGSNTGVFVGVMANEWAHLHLSDYREITAQAGSGNGYFMTANRLSYQLDLRGPS
                     LAVDTACSSSLVAVHLAAQALRNGECDQAVAGGVNLTLTPAVNVFYTKAGLAAPDGRC
                     KPFSGSADGIGRGEGVAVVVLRRLEDALDAGLPIYAVIKGGAVNSDGRSNGITAPNRW
                     AQQQVAATAYRAAGIVPAQIAFLEAHGTGTLLGDMIEAKALADAHRSGRDEPCAIGSI
                     KGNLGHTEGAAGIASLIKIVLSLHHRVVPASRFAETENAQLRLAAGGLRLLAEAMELP
                     QETVYAGVSSFGIGGTNCHMVLASAPAVEPAPTATGNGVLTISADSPEALRRNVLQLA
                     SDIEQSEAPLAQLCWTTNRVKASGKTRLALVARDRGEAIAALRGDLEIGTTAGMSAGW
                     LFSGQGTQYPGMASAPYESSDAFRDAFDQVDAAMLPHLGVRIAAAMNDSRVNSTEFAQ
                     PAIFALQYAQAQALLRLGAEPAWLLGHSIGEYAAAAIAEVFSLDDACRLVVARGRLMQ
                     QLPAGGGMLAVRTSASEVAGLSLDLAAVNGPDEFVLSGAMEAIDDAAETLDRAGITAR
                     RLNVSHAFHSQLMKPITAKFASIAAEISYQLPAFPIFSTLHGRLLGDDEPMDADYWTD
                     HIGATVRFADAAAAALQAEPTHLVELGAKRTLAPMITRAHNDVPPALTVATGLTETVA
                     ALYRDGLNPDWNLLYANEARVAHRLSGYSFGTANRFWIKEQAQAATSGSAQLAISTTA
                     TVSKDSTMDNLIALFREQAAVLAAYGQGNAPAIAAAEPAPQSNPAVDVAAIVRSEIAR
                     VSGFPENKLRTTQTIADDLGFDSIMVTDLFSGLTRKIPGVTIDPSGFGRATRIADVIA
                     MSGGQAQESAAPQARPTVTPQFRIGEFEEVKALADRKALAEAVGVDNPYFLVNDGVTR
                     DTSIINGAEVINFSSYNYVGMSGHPAVAEAVSDAVRQWGSSCSASRLLSGEKPVHREL
                     ELELAKLLGTEDAMALVSGHATNVTVIGHLLGEGDLVIHDSLAHDSIMQGCKLSGATR
                     RPFPHNDPAVLDELLTSIRHQYRRVLIIIEGVYSQDGDIPDLPAFIEVKRKHQALLMI
                     DEAHSIGVLGATGGGIGEHFGVDRADVELWSGTMSKALAGCGGYVGGSRELIEFLKYT
                     TPGFIYSVGMPPPTAAASLAAIRAIRSEPETLERLREMSALFLTLAREAGLDTGDSEG
                     TPVIPCIVGSSVTALKLSNALLQRGINANPILYPAVPEDKARLRFFVTSCHSEEQIRY
                     TVKTLAEELAILSEASEGA"
     gene            complement(5107..5373)
                     /locus_tag="VFC01_22035"
     CDS             complement(5107..5373)
                     /locus_tag="VFC01_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZN82338.1"
                     /translation="MNANRRPILRWLTAQLASYLEVPATAIDPMVPLAEMGVDSVHAV
                     SLVGDVEAHFDIDVDPTMIFDYPTLAHIAEYIDESVAEQRGAVA"
     gene            complement(5370..7115)
                     /locus_tag="VFC01_22040"
     CDS             complement(5370..7115)
                     /locus_tag="VFC01_22040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HZN82339.1"
                     /translation="MIDSTAPSHRYNLAEHLRSRFETYGDSRTFTFLHESGRTLAEEV
                     RTFHDLDRGAREVAAWLSSRYDARQPVLLLFEPGLDFWKAFLGCLYAGVTAIPAPLPH
                     DQRSMQRVTGILRDADSRLALTTAKLRDLLTAGIDGPGLDRPIVFEAIDELQLADPDS
                     WNAPHITSDTVAFLQYTSGSTGDPKGVAVTHANMLHNAATISQALGFSDASVITGWIP
                     HFHDMGLISVLTALCAGANLVAMSPLVFLKQPIRLLKVISDYRGTATAAPNFAYDLIA
                     RRVTPEQLAGLDLSSLEVALNGAEPVRRRTIERVTEMLAPAGFASSAMRPAYGMAEVT
                     LMATISGGVQRYLDADADALEHNHYTPAKGRAVSLVSSGEPAPGIDVHIVDPETLHEL
                     PEDRVGEIWLRSASVAAGYYHRPAETIERFNAYSADGAGPFLRTGDLGLLHEGELYVT
                     GRRKDLLIVNGRNLYPQDIEEFVQGLHPAVAGSRGVAIAVDINDAERLVLIQAVKHEL
                     LGDTNYGELAAAVKSAVARDFEIPAPGVVFVNRAGIHLTTSGKVQRASMRAAFLNAAL
                     TDVVHESAEALGASK"
     gene            complement(7112..8101)
                     /locus_tag="VFC01_22045"
     CDS             complement(7112..8101)
                     /locus_tag="VFC01_22045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN82340.1"
                     /translation="MATATTAPAPVSPGAPSFLRSRPHAERIERIVTVRDGVQLSVRD
                     QGPTRAEATVVLLHGLCLEKGSWAGQIRHLTRQWGDRVRIISYDHRGHGKSGSAPNRT
                     YTVAQLAEDLADVLTALRVTGPLTLAGHSMGGMVALAYLGRPPADRPVEPHGLVLAAT
                     AAGRLAEHGVARLLASPAMDVLCGLAAFAPQRALRLLTKPLCATLALQIGGHARSTLT
                     ALFANALTATTSLDAAVGFLPGLRSYDQTSTLGSICAQTIVLSGGVDLLTPPSHSHHL
                     ASAIPGATHEHLPAAGHMLLQEAARAVTDAISRTIATRIHVPAQRYSAHLRTA"
     gene            8291..9598
                     /locus_tag="VFC01_22050"
     CDS             8291..9598
                     /locus_tag="VFC01_22050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HZN82341.1"
                     /translation="MPEILIASLSPAGHIAPLLNVAQGLVARGDRVTVLSSADHAAKI
                     RAVGAIPQAIPAEADFDMTRLDIDLPGRTETSGIKRVNFDIVRLFVQPMPHQTRVLSQ
                     LMNQTKFDAVIVDAGFFGILPFLLGDRAARPPVLVYATTPLMISSRDTAPSGMGLPPS
                     SSALGRLRNRALHLVSQKVLLRQSQNAANYLLDHLNSRRLPTFLLDAGLLADRYISPT
                     VPEFDYPRSDLPAQVRYVGAVHPGPSNGFRLPPWWAALDADRPVVHVTQGTIDNADLG
                     RLVEPTIEALGDEDVIVVATTGGRDISEVKVTLPVNTLVAEYIPHDLLLSKVDVMITN
                     GGYGAVQRALSMGVPLVVAGNTEDKPEVAARVAWTGAGINLRTGTPTPGAIRAAVRDV
                     LGDGRYLRGARDLEAAFARRDGVAEIAALVDEVISERSAVVDR"
     gene            complement(9993..11273)
                     /gene="clpX"
                     /locus_tag="VFC01_22055"
     CDS             complement(9993..11273)
                     /gene="clpX"
                     /locus_tag="VFC01_22055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZN82342.1"
                     /translation="MARIGDGGDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELADADDVKLDELPKPVEIREFLEGYVIGQDTAKRTLAVAVYNHYKRIQAQEKT
                     RDSRAEPVELAKSNILMLGPTGCGKTYLAQTLAKMLNVPFAIADATALTEAGYVGEDV
                     ENILLKLIQAADYDVKRAETGIIYIDEVDKIARKSENPSITRDVSGEGVQQALLKILE
                     GTQASVPPQGGRKHPHQEFIQIDTTNVLFIVAGAFAGLEKIVSDRVGKRGLGFGAEVH
                     SKAEIDTQDHFAEVMPEDLIKFGLIPEFIGRLPVVASVTNLDKDSLVQILSKPKNALV
                     KQYTRLFEMDGVELEFTDDALEAIADQAIHRGTGARGLRAIMEEVLLPVMYDIPSRDD
                     VAKVVVTKETVQDNVLPTIVPRKAARPERRDKSA"
     gene            11538..12419
                     /gene="mmuM"
                     /locus_tag="VFC01_22060"
     CDS             11538..12419
                     /gene="mmuM"
                     /locus_tag="VFC01_22060"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HZN82343.1"
                     /translation="MFAAPHDTVLLTDGGLATELEARGHDLSDDLWSARLLVDTPEEI
                     VAVHCAFFRAGASIATTASYQASFDGFAQRGISRDEAAGLLRRSVELAKAARDEVGGE
                     RWVAASVGPYGAALANGEEYVGRYGLSVAQLADWHRPRLEVLAGAGADVLAVETVPDV
                     DEAEALVGLIHELGVPAWLSYTIAGTTTRAGQPLADAFAVAAGVPEIVAVGVNCCAPD
                     DVLAAVEIAREVTGKPVIAYPNSGEAWDGVRRTWIGSSGWSAELAPRWVAAGARIIGG
                     CCRVRPDDIATLALALR"
     gene            complement(12473..13120)
                     /gene="clpP2"
                     /locus_tag="VFC01_22065"
     CDS             complement(12473..13120)
                     /gene="clpP2"
                     /locus_tag="VFC01_22065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent CLP protease proteolytic subunit
                     ClpP2"
                     /protein_id="HZN82344.1"
                     /translation="MTDYTDPRMQPQARYILPSFIEHSSFGVKESNPYNKLFEERIIF
                     LGVQVDDASANDIMAQLLVLESLDPDRDITMYINSPGGSFTSLMAIYDTMQYVRADIQ
                     TVCLGQAASAAAVLLAAGTPGKRLALPNARVLIHQPALAGVIQGQFSDLEIQAKEVER
                     MRTLMETTLAHHTGKDAAVIRKDTDRDKILTAEEAKDYGIIDTVLEYRKLSAQTA"
     gene            complement(13117..13704)
                     /locus_tag="VFC01_22070"
     CDS             complement(13117..13704)
                     /locus_tag="VFC01_22070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HZN82345.1"
                     /translation="MRGAAQGLNLVDSVYERLLSERIIFLGSQVDDDIANKLCAQILL
                     LAAEDPTKDINLYINSPGGSISAGMAVYDTMVLAPCDIATYGMGMAASMAQFLLAAGT
                     KGKRFALPHARILMHQPLGGVTGSAADIAIQAEQFTVIKKEMFRLNAEFTGQPVERIE
                     ADSDRDRWFTAQEALEYGFVDHIITSAHVNGVSPS"
     gene            complement(13830..15224)
                     /gene="tig"
                     /locus_tag="VFC01_22075"
     CDS             complement(13830..15224)
                     /gene="tig"
                     /locus_tag="VFC01_22075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZN82346.1"
                     /translation="MKSTVEKLSPTRVRINVEVPFTELQPDIDTAFKQMAKQIRMPGF
                     RPGKVPVKLLEARVDKQAMFDQVIGDAVPARYTEAVTTSDVRPLGQPEIDITNKEYGQ
                     DLTFTAEVDVRPEIEIPELEGLKITVDPIEVNDEEVDTELQNLRARFGTLKGVERAAE
                     NGDFVSIDLSATVDGEDVPDAKTEGLSHEIGSGQLIDGLDEAIVGLKEGETRVFTTTL
                     AAGEHAGQEAQVTVTVKSVKERELPEPDDEFAQLASEFDTIDELKSSLTEQVQRVKRI
                     QQAEQIRDKALEVLLEQTDVPLPENIVQAQVDDTLHNAIHGLDHDEKRFEDTLKEQGS
                     SREEFDTDNRTNAEKAVKTQLLMDAVADKLDIQVGQNDLTERLVLMSQQYGMEPQQLL
                     QFLQQNNQLPAMFADVRRGLTVAAVVHGATVSDTDGNVIDTTEFFGPSGEQAAEAAEA
                     AEETTSDEVTADAK"
     gene            complement(15263..15336)
                     /locus_tag="VFC01_22080"
     tRNA            complement(15263..15336)
                     /locus_tag="VFC01_22080"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15300..15302),aa:Pro,seq:tgg)
     gene            15451..15521
                     /locus_tag="VFC01_22085"
     tRNA            15451..15521
                     /locus_tag="VFC01_22085"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15483..15485,aa:Gly,seq:tcc)
     gene            complement(15554..>16312)
                     /locus_tag="VFC01_22090"
     CDS             complement(15554..>16312)
                     /locus_tag="VFC01_22090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HZN82347.1"
                     /translation="RASAGRGPAPAAPPGGAAQTYLFRDEFDGPAGSAPDYSKWEPAL
                     AREDMEDPTFWELPENVGQYRDDRRNVFLDGKSNLVFRAAKDGNTYYSGKLFGKYRGP
                     IGTTWEARIKLNCLTPGSWPAWYLANNNPVNGGEVDLMEWYGNGHWAAGTTVHALLNG
                     GEHVSHGITVDSGWHTWRVQWDNAGMRFWKDYVDGAQPYFDVPANSLPDWHFNEPGYT
                     LFPILNLAVAGSGGGDPSAGTYPADMLVDWVRVW"
BASE COUNT         2623 a         5150 c         5529 g         3010 t
ORIGIN      
        1 gacggtgccg tcatgtgcga cgacttgcaa caccagattc ggtgtcgtgc tgaaggtctc
       61 gtcaaggaaa tcggcgactt gagtcgattc agctgccgga tcgacgaaac caccgacacc
      121 gagcttctct gacaccgtgc cgccgaagac gatgctgaaa ccaagcaacg ccacgaccgc
      181 aaggaggacc acctttggcc gccgcacaac gaacgcggct aaccgagtca gcattgtgca
      241 ccaccgatct ttgcgagctg acgccgcagg gtggcgagca actgtggccc gtcaccaagg
      301 tagaagtgcc ccccgcgaag gatctgaaca gagaactcct ccgttgtacg cgaacgccat
      361 tcgtgcagat cgggttcgct gacgaggggg tcgtcgctgc caccgaacgc gtggatcggg
      421 caaggcagcg gcacgacatc ggcatcctcg tcgctcgcgc acagccgcag gtcgacccgg
      481 gtcgcggcga cggccctgtc gtgaagtgcg ggccattgtg cgagctccgg cggcatccca
      541 cccaaatcgg acagcagggc cgccagctgc agatcatctt ccaccggggg tatcgggggc
      601 ggaagatgcg gtggcgcata cgaagacacc acgagtgcac gcggcagatt cgcacccgca
      661 gctgctcgta gacagaccaa ccggtacgcc agcagcgcac cgaaactgtg gccgaagaac
      721 acatgtgcac cgtccagttc cgatcggagc tgatcgtgca gcaactccac cagctggctc
      781 agcgtcgcga agcgctcacg gttctcgacc tcgacgggaa ccacctcgat gcgcggacca
      841 agggcccggt tccaggcgtt gaacatcgcc cgtccgccgc cggcgtggtg aaagcagaac
      901 aaccgcaccg tctcacgcgc cttccgaggc ttcagagaga atcgcgagct cttcggcaag
      961 ggtcttcacc gtgtagcgga tctgctcttc cgaatggcag ctggtgacga agaatcgcag
     1021 ccgcgctttg tcttccggca cggccggata gaggatcggg ttggcgttga tgccgcgctg
     1081 cagcagcgcg ttcgacagtt tcagggcggt gacggaactg cccacgatgc atgggatcac
     1141 cggggtgccc tcgctgtcgc cggtgtccag gccggcttcg cgtgccagcg tcaggaacag
     1201 agccgacatc tcgcgcagcc gttccaatgt ctccggctcg ctgcggatcg cacggatcgc
     1261 cgcgagcgac gccgcagcgg tgggcggtgg catgccgacg ctgtagatga agcccggcgt
     1321 cgtgtacttg aggaactcga tgagctcccg gcttccgccg acataaccgc cgcagccggc
     1381 cagcgccttc gacattgtgc cggaccacaa ttcgacgtca gcgcggtcga cgccgaagtg
     1441 ttcgccgatg ccgccaccgg tcgcgccgag cacgccgatg ctgtgtgcct cgtcgatcat
     1501 cagcagggcc tgatgcttgc gcttgacctc gatgaaggca gggaggtcgg gaatgtcacc
     1561 gtcctggctg tagacgcctt cgatgatgat cagcacccgg cggtactggt gacgaatgct
     1621 cgtgagcagc tcgtcgagca cggcggggtc gttgtgcgga aacgggcggc gcgtggcacc
     1681 ggagagcttg cagccctgca tgatgctgtc gtgcgcgagg ctgtcgtgga tcaccagatc
     1741 gccttcgccg agcaggtgcc cgatcacggt gacgttggtg gcgtggccgc tgaccagcgc
     1801 catcgcgtcc tcggtgccga gtagcttcgc cagctcgagc tccagttccc ggtgcacagg
     1861 cttctcaccg gagagcaggc gtgacgccga gcaagagctg ccccattggc gcaccgcgtc
     1921 cgacaccgct tcggccaccg cgggatggcc cgacatgccc acgtagttgt agctggagaa
     1981 gttgatgacc tccgcgccgt tgatgatcga ggtgtcgcgg gtgacgccgt cgttcaccag
     2041 gaagtagggg ttgtcgacgc cgactgcctc ggcgagagcc ttccgatcgg ccagcgcctt
     2101 gacttcctcg aactcgccga tccggaactg tggtgtgacg gttggccgag cttgcggtgc
     2161 tgcagactcc tgagcctgac cgcccgacat cgcgatcacg tcggcgatcc tcgtggctcg
     2221 cccgaatcca cttgggtcga tcgtcacgcc cgggatcttg cgggtcagcc cgctgaacag
     2281 atcggtcacc atgatcgagt cgaatccaag atcgtcggcg atggtctgcg tggtacgcag
     2341 cttgttctcc gggaatccgc tgacccgcgc gatctcggag cgcacgattg cggcgacgtc
     2401 gacagccgga ttcgactgcg gcgcaggctc ggccgctgcg atggctgggg cattgccctg
     2461 gccgtatgcg gcgagcaccg cggcctgttc acggaagagg gcgatgaggt tgtccatggt
     2521 cgagtccttc gagacagtgg cggtggttga aatggcgagc tgtgcggagc cagatgtcgc
     2581 cgcctgagct tgttccttga tccagaaccg gttggcggtg ccgaacgagt agccgctgag
     2641 ccgatgagcc acccgagctt cgttggcgta cagcaggttc cagtcgggat tgaggccgtc
     2701 gcggtagagc gcggcgacag tctcggtaag gccggtcgca acggtgaggg cgggcggcac
     2761 gtcgttgtgc gcccgcgtga tcatcggggc gagggtgcgc ttggcgccca actcgacgag
     2821 gtgcgtcggc tcggcctgca gcgctgcggc cgccgcgtcg gcgaaccgaa cggtagcgcc
     2881 gatatggtcg gtccagtagt ccgcgtccat cggttcgtcg tcgccaagca ggcggccgtg
     2941 cagcgtggaa aagattggga acgcgggcag ttggtaggag atctcggcgg ctatcgaggc
     3001 gaactttgcg gtgatcggct tcatcagctg cgagtggaat gcgtgcgaca cgttgagccg
     3061 gcgcgcggtg atgccggcgc ggtccagcgt ctccgcggcg tcgtcgatcg cctccatcgc
     3121 acccgacagc acgaattcgt cggggccgtt caccgcagcg aggtcgagcg ataggccggc
     3181 cacctcgctg gcactggtac ggaccgcgag catgccgccg cccgcaggca gctgctgcat
     3241 caggcggcca cgggctacga ccagacggca cgcgtcgtcg aggctgaaca cctcggcgat
     3301 ggcggctgcc gcatattcgc cgatgctgtg tccgagtagc caggccggtt cagcaccgag
     3361 ccgaagcagt gcttgtgcct gtgcgtactg cagcgcgaag atcgcgggct gggcgaactc
     3421 ggtgctattc acccggctgt cgttcatggc cgcggcgatc cgcacgccga ggtgcggcag
     3481 catcgcggcg tcgacctgat cgaacgcgtc ccggaacgca tcgctgctct cgtagggcgc
     3541 agacgccatg ccggggtact gcgtgccttg gccgctgaac agccagcccg ccgacatccc
     3601 cgcggtggtg ccgatttcga ggtcgccgcg cagtgccgcg atggcctcgc cgcggtcgcg
     3661 ggcgacgagc gctagtcgcg tcttgccgga cgctttgacg cggttggtcg tccagcacaa
     3721 ctgggcgaga ggagcctcac tctgctcgat gtcgcttgcg agttgtaata cattgcgacg
     3781 cagcgcttcc ggggagtcag ccgagatagt gaggacgccg ttgccggttg ctgtcggcgc
     3841 aggttcaact gcaggggcgc tggcgagcac catatggcag ttcgtgccgc cgataccgaa
     3901 gctggagacg cccgcataga cggtttcctg cggcaattcc atcgcctcgg cgagcagccg
     3961 gagtccccca gcggccagtc ggagttgggc gttctccgtt tcggcgaacc gggacgcagg
     4021 caccactcgg tggtgcaggc tcagcacaat cttgatcagg ctggcgatac cggctgcgcc
     4081 ctcggtgtgg ccgaggttgc ccttgatcga cccgatcgcg cagggctcgt cgcgacccga
     4141 gcggtgagcg tcggcaaggg ctttcgcctc gatcatgtcg ccgagaaggg ttccggtgcc
     4201 gtgcgcctcg aggaaggcga tctgcgcggg cacgatcccg gcggcccgat acgccgtggc
     4261 ggcgacttgc tgctgcgccc agcggttcgg cgcagtaatg ccattgctgc gaccgtcgct
     4321 gttgaccgcg ccgcccttga tgacggcgta gatcggcaag cctgcgtcga gtgcatcttc
     4381 cagccggcgc agcacgacga cggctacgcc ctcgccccgg ccgataccgt cagccgagcc
     4441 gctgaatggt ttgcagcgcc cgtcgggggc ggccaggccc gctttcgtat agaagacgtt
     4501 gacggcaggg gtcagtgtga ggttgacgcc ccccgcaact gcttggtcgc actcgccatt
     4561 gcgtaacgcc tgcgcggcca gatgcaccgc gacaagcgac gacgagcacg cggtgtccac
     4621 cgcgaggctc gggcctctga ggtcgagttg gtaggagagc cggtttgcgg tcatgaagta
     4681 gccgttgccc gaaccggcct gcgccgtgat ttcgcggtag tcgctcaagt gcaggtgggc
     4741 ccattcgttg gccatcactc cgacgaacac tccggtgttg gagccagcct gcccgcgtgg
     4801 gtccagggtg gcgttttcca ttgcccgcca agtcgtttgc aggaggaggc gctgctgggg
     4861 atccatcgcc tcggcctcgc gcggtgtgat gccgaagaag tcgttgtcga acgcgtcggc
     4921 gtcgctgatc agtccgccgg tccggtggtt gagggtgccg tcgtcgtgga gttcggcagc
     4981 gttccagcgc tcggccggga tctcgtcgat tccgtcagcg ccctcaagga gcagcttcca
     5041 cagggcagtg ggatcgcccg ccttcgggaa gcggcagtcg atccccacga ccgcgacgtc
     5101 ggccatttat gccaccgccc cccgctgctc ggcgacggat tcgtcgatgt attcggcgat
     5161 gtgggcgagg gtcgggtagt cgaagatcat cgtcgggtcg acatcgatgt cgaagtgtgc
     5221 ctccacgtct ccgacgaggc tgaccgcgtg caccgagtcg acgcccatct ccgcgagagg
     5281 aaccatcggg tcgatcgcgg tggcgggcac ctcgaggtag gacgcgagct gcgcggtgag
     5341 ccagcgcagg atcgggcggc ggttggcgtt catttggagg ctcctagggc ttcggcgctt
     5401 tcgtggacta catcggtcag tgcggcgttg aggaacgctg cccgcatcga tgcgcgctgc
     5461 acctttccgc ttgtggtgag gtggatgccg gcgcggttga cgaaaaccac tccgggggct
     5521 gggatttcga agtcgcgggc gacggcactc ttaaccgcgg cggcgagctc gccgtagttc
     5581 gtgtcaccca gcaactcgtg cttgaccgcc tggatcaaga ccaagcgttc cgcgtcgttg
     5641 atgtcgacgg cgatcgcgac cccgcgtgaa ccggccacgg ccgggtgcag gccttgcacg
     5701 aactcttcga tgtcctgcgg atacaggttt cgaccgttga cgatcaagag gtccttgcgg
     5761 cggccggtca cgtacagctc accctcgtgc aggagcccga ggtcgcccgt gcgcaggaac
     5821 ggccccgcgc cgtcggcgct gtaggcgttg aaccgttcga tggtctccgc agggcggtgg
     5881 tagtagccgg ccgcgacgct cgcgctgcgc agccagatct cgccgacccg gtcttctggc
     5941 aactcatgca gtgtctccgg gtcaacgatg tgcacgtcga taccgggtgc cggctcgccc
     6001 gaactcacga ggctgaccgc acggcccttc gcgggtgtgt agtggttgtg ctcgagtgcg
     6061 tcggcgtcgg cgtcgaggta ccgctggacg ccgccgctga tggtggccat cagtgtcacc
     6121 tcggccatgc cgtacgcggg acgcattgct gaagacgcga atccggccgg agcgagcatc
     6181 tcggtgaccc gctcaatggt gcgacggcga accggctcgg ccccgttgag cgccacctcc
     6241 aacgaggaaa ggtcaaggcc ggccagttgt tctggggtca cccgccgcgc gatcaggtcg
     6301 taggcgaaat tcggtgcggc ggttgcggtt ccgcggtaat cgctgatcac cttgagcagc
     6361 cggatgggct gcttgagaaa caccagaggc gacatcgcga cgaggttcgc gcccgcgcag
     6421 agagcagtca gcacgctgat gaggcccatg tcgtgaaaat gcgggatcca gccggtgatg
     6481 accgacgcgt cggaaaagcc gagggcctga gaaatcgtgg cggcgttgtg cagcatgttg
     6541 gcgtgggtga cggcgacgcc cttggggtcg ccggtagagc cggacgtgta ctgcaggaag
     6601 gcgacggtgt cggaggtgat gtgcggtgcg ttccacgagt ccgggtcggc cagctgcaat
     6661 tcatcgatgg cctcgaacac gatgggacgg tcaaggccgg gcccgtcgat gcccgcggtg
     6721 agtaggtctc ggagcttcgc tgtggtcagc gccaggcggc tgtcggcgtc gcgaagtatc
     6781 ccggtgaccc gctgcatgct ccgctggtcg tgcggcagtg gcgcaggtat cgcggtgact
     6841 ccggcataga ggcagccgag gaacgccttc cagaagtcaa gcccgggttc gaacaacagc
     6901 agcaccggct gcctggcgtc gtaccgcgag ctcagccaag cagccacctc gcgcgccccg
     6961 cggtcgaggt cgtggaaagt ccttacttcc tcggccaggg ttcgtccgga ctcgtggaga
     7021 aacgtgaaag tgcggctatc gccataggtt tcgaatcggg agcgaaggtg ctcggcgagg
     7081 ttgtagcggt gcgatggtgc ggttgagtcg atcatgctgt cctcagatgc gccgaatacc
     7141 gttgtgcggg aacgtgaatg cgcgtggcga tcgtgcggct gatggcatcc gtgacggcac
     7201 gcgcggcctc ttgcaggagc atgtggcctg cagcgggaag gtgctcgtga gtggcgccag
     7261 gaatggcgct ggccaggtgg tgcgagtgag acggtggggt gaggaggtcg actccgccgc
     7321 tgaggacgat cgtctgagcg cagatggagc cgagcgtgga agtttggtcg taggaccgca
     7381 ggccgggcag gaacccgacg gcggcgtcga gagatgtcgt ggcggtcagg gcattggcga
     7441 acagcgccgt aagcgtggag cgagcgtggc cgccgatctg gagggccaac gtggcgcaga
     7501 ggggcttggt aagcagccga agggcgcgct gaggtgcgaa ggcggcgagc ccacacagca
     7561 cgtccatcgc gggactggcc aggaggcggg caacgccgtg ctcagcgagt cggccggccg
     7621 cagtcgcggc gaggaccagt ccgtgcggct ccaccggccg gtcagccgga ggccggccaa
     7681 ggtaggcgag ggcgaccatc ccgcccatcg agtggccagc gagggtgagc gggccggtca
     7741 cgcgaagggc tgtcaggaca tcggctaggt cttcggcgag ttgcgcgacc gtgtaggtgc
     7801 ggttcggggc cgaacccgac ttgccatggc cgcggtggtc atagctgatg atccgaaccc
     7861 ggtcgcccca ttgacgggtt aagtgacgga tctggccggc ccatgatcct ttctcgaggc
     7921 aaaggccgtg caggagcacg acggtggcct cggcgcgggt tggcccttgg tcccgcactg
     7981 acaactgcac tccgtcgcgg acggtgacga tgcgttcgat gcgctcagcg tgcgggcggc
     8041 tacgcaggaa actcggcgcg cccggagaga ccggggcggg tgccgtggtt gctgtagcca
     8101 tgacgaaatg gtcatggatc tggtagtggc tccgtaatcg tcgggacgcc agatttcact
     8161 actgaacggc gagactttcc tacccgccag gtaaggcctc cgatgcgcca cccatctctg
     8221 cctgcaatct ccatcagatc tccataaagc ctccaagctc gcgtgcgctg gtttggcgag
     8281 catcgaggcc atgccagaaa tcctcattgc ctcactgtcg cctgccggtc acatcgcgcc
     8341 gctgttgaac gtcgcgcagg gcttggtggc ccgcggcgat cgtgtcacgg ttctcagctc
     8401 cgccgaccac gccgcaaaga tccgcgccgt cggcgccatc ccgcaggcga taccggcaga
     8461 ggccgacttc gacatgaccc gactcgacat cgacttgccc ggccgcacgg agacgtctgg
     8521 catcaagcgg gtcaacttcg acatcgtccg gctgttcgtg caaccgatgc cgcaccagac
     8581 cagggtgctg tcgcagttga tgaaccagac aaagttcgac gccgtcatcg tcgacgcggg
     8641 cttcttcggg attctgccgt tcctactcgg tgaccgcgcg gcccgcccgc cggtgctggt
     8701 ctacgccacc accccgttga tgatcagtag ccgcgacacc gcgccgagcg gcatgggcct
     8761 gccaccctcg tcaagcgcgc tcggacggct gcgcaaccgc gcattgcacc tggtgtcgca
     8821 gaaagtcttg ctgcgtcaat cgcagaacgc cgccaactac ctgctggacc acctgaacag
     8881 ccgccggcta ccgacgttcc ttctggatgc cggtctgctt gcggatcgtt atatctcgcc
     8941 gacggtcccg gagttcgact atccgcgcag cgatctaccc gcccaggtgc gctacgtcgg
     9001 tgcggtccat cccgggccgt cgaacggctt tcggctgccg ccgtggtggg ccgcgctcga
     9061 cgctgaccgg ccggtcgtcc acgtcaccca gggcacgatc gacaacgccg atctgggccg
     9121 actggtggag ccgacgatcg aggcgctcgg cgacgaggac gtcatcgtgg tagccaccac
     9181 gggcggccgg gacatctcag aggtgaaggt taccctgcca gtgaatacgc ttgtcgccga
     9241 atacattccg catgatctgc tgctatccaa ggtcgacgtg atgatcacca acggcggcta
     9301 cggcgcggtg cagcgggcgc tgtcgatggg cgttccgctg gtcgtcgcgg gcaacaccga
     9361 agacaagccg gaagtcgccg cacgagtggc gtggaccggc gcaggcatca acctgcgaac
     9421 cgggacgccg actccgggtg ccatccgcgc cgcggtgcgt gacgtgctcg gcgacggccg
     9481 ctacctgcgc ggcgcccggg acctcgaggc cgcgttcgcc cggcgcgacg gtgtcgccga
     9541 gattgcggcg ctcgtcgacg aggtcatcag tgaacgcagt gcggtggtgg accgttgaca
     9601 cgcatgtggg tgatacagct ttacttcgca aaccggtcgc tgaccgtcac cgtcggccct
     9661 cgcagggcac tgtgatgagc acctgtgccg acccgttcgg cacgccgccc gtcgagcagt
     9721 cgcgcaaccc ggccccaaag tcgcgtagtc gtctactcgt gtgtaccgcg actatccgga
     9781 gaagtcttga gtcatggtta ccgagctgca gttcgtcgcg gtagcggcgt tcttgatctg
     9841 ggggttggca gcgtcgttgt gtggcctgct caagccggac gatgacgcga gtgtggactt
     9901 aagacacgcc gagtccagag accgcgtacg ttaggtcgac actcggcgcg gaggagcaag
     9961 agccccgcca gtgcggggct cttgtctaga acctaggcgc tcttgtcgcg ccgctctgga
    10021 cgagccgcct tgcgcggcac gatcgtcggc agcacgttgt cctgcacggt ctccttggtg
    10081 acgaccacct tggcgacgtc gtcgcggctg gggatgtcgt acatcaccgg cagcaggact
    10141 tcctccatga tggcgcgcag gccgcgggcg ccggtgccgc ggtggatggc ctgatcggcg
    10201 atggcctcca gcgcgtcgtc ggtgaactcc aactccacgc cgtccatctc gaagaggcga
    10261 gtgtactgct tgaccaacgc gttcttcggc ttggacagga tctgcaccag cgagtccttg
    10321 tccaggttcg tcaccgacgc cacgaccggc agtcggccga tgaactccgg gatcagcccg
    10381 aacttgatca ggtcttctgg catcacttcg gcgaagtggt cctgcgtatc gatctcggcc
    10441 ttggagtgca cctcggcgcc gaagccaagc ccgcgcttgc cgacccggtc ggagacgatc
    10501 ttctccagcc cggcgaacgc acccgcgacg atgaacagca cgttggtggt gtcgatctgg
    10561 atgaactcct ggtgcggatg cttgcgcccg ccctgcgggg gcaccgaggc ctgcgtcccc
    10621 tcgaggatct tcaacaaagc ttgctggacg ccctcaccgg agacgtcgcg ggtgatcgac
    10681 gggttctcac tcttgcgggc gatcttgtcg acctcgtcga tgtagatgat gccggtctcg
    10741 gctcgcttca cgtcatagtc ggcggcctgg atcagcttga gcagaatgtt ctcgacgtcc
    10801 tcaccgacgt agccggcctc ggtcagcgcg gtggcgtcgg cgatcgcgaa tggcacgttg
    10861 agcatcttgg cgagcgtctg cgcaaggtaa gtcttaccgc agccggtggg cccgagcatc
    10921 aggatgttgg acttggccag ctcgacgggc tcggcgcggg aatcgcgcgt cttctcctgg
    10981 gcctgaatgc gtttgtaatg gttgtatacg gcgactgcca gcgtgcgctt ggcggtgtcc
    11041 tgacctatga cgtaaccctc gaggaattcg cggatttcga cgggcttggg cagctcgtca
    11101 agcttgacgt cgtcagcgtc ggccagttcc tcttcgatga tctcgttgca cagatcaatg
    11161 cattcatcac agatatagac gcctgggccc gcaatgagct tcttgacctg cttttgactc
    11221 ttgccacaga acgagcactt cagcaggtca ccgccgtctc caatgcgcgc catggtggtg
    11281 gggtcctact ttcctagcag tcggtaatga agcgtgttta cgagttacct cggtgttgga
    11341 cccgacgcta cccgtatgtt ccggcgcggt gcgaccgata aagccgaatc gcgtcggtgg
    11401 tatttatatg tgttcgcaga acatatcgcc tgactcgcgg tctaaccccc tggaacccgc
    11461 cactgtgtcc ctggcgtgtc gccgccgtta tcggaccgag aggccgcccc cgaagtggcc
    11521 gtagagtttg cgatgtggtg ttcgctgcgc cccacgatac cgtgctcctg accgacggcg
    11581 gcctggcgac ggagctggag gcccgcggcc acgatctgtc cgacgacctg tggtcggccc
    11641 ggctgctggt cgacacgcct gaggaaattg tcgccgtaca ctgcgcgttc ttccgcgcgg
    11701 gagcgtcaat cgccacgact gcgagctatc aggcgtcgtt cgacgggttc gctcagcgcg
    11761 gaatctctcg tgacgaggcc gccgggctac tgcgccgaag cgtcgagctc gcgaaggccg
    11821 cccgcgacga ggtcggcggt gaacgctggg tggccgcatc cgtcgggccg tacggcgctg
    11881 ccctcgccaa cggcgaggag tacgtcggcc gctacgggct ttcagtcgca cagttggcgg
    11941 attggcaccg accgcggctg gaggttctcg ccggcgccgg agccgacgtg ctggctgtcg
    12001 agacggtccc cgatgtcgac gaggccgaag cgctcgtcgg cctcattcac gaacttggtg
    12061 ttccggcgtg gctgtcctac accattgcgg ggacgacgac gcgcgccggg cagccgcttg
    12121 ccgacgcgtt cgcggtggct gccggagtgc cggaaatcgt tgcggtgggc gtgaattgct
    12181 gcgcgcccga tgatgtgctg gccgccgtcg agatcgcccg cgaggtcacc ggcaagcccg
    12241 tcatcgccta tcccaacagc ggcgaagcgt gggatggcgt tcggcgaacg tggatcggat
    12301 cgtcaggctg gtcggccgag ctcgcgcctc gctgggtggc ggcgggcgca cgcatcatcg
    12361 gcggatgctg ccgcgtcagg ccggacgata tcgccacgct tgcgttggct ctgcgctgag
    12421 ggcgtagtcc tctcgcactt ctgcgccgta gacgcagagc caacaaagac ggctaggccg
    12481 tctgagccga cagcttgcgg tactcgagga ccgtgtcgat gatcccgtag tccttggcct
    12541 cctcggccgt caggatcttg tcgcggtcgg tgtccttgcg gatgaccgcg gcatccttgc
    12601 cggtgtggtg ggccagagtg gtctccatca gggtccgcat ccgctcgacc tccttggcct
    12661 ggatctcgag atcggagaac tggccctgga ttacgccggc caacgcgggc tggtggatca
    12721 acacgcgagc gttcggcagc gccaggcgct tgcccggcgt accggccgcc agcagcaccg
    12781 cggcggccga tgcggcttgg cccaggcaca cggtctgaat gtcggcgcgg acgtactgca
    12841 tggtgtcgta gatcgccatc agcgaggtga acgagccacc cggcgagttg atgtacatcg
    12901 tgatgtcgcg gtcgggatcc agcgactcca acaccaacag ctgcgccatg atgtcgttgg
    12961 ccgaggcgtc atcgacctgc acgccgagga agatgatgcg ttcctcgaac agcttgttgt
    13021 acgggttgga ttccttgacg ccgaagctcg agtgctcgat gaacgacggg aggatgtagc
    13081 gagcctgcgg ctgcatccgg ggatcggtgt aatcggtcat gatggcgaca ctccgttgac
    13141 gtgggcgctg gtgatgatgt ggtcgacgaa cccgtactcg agggcctcct gcgcggtgaa
    13201 ccagcgatcg cgatcggagt cggcctcgat acgttccacc ggctggccgg tgaactcggc
    13261 gttgagccgg aacatctcct tcttgatgac ggtgaactgc tcggcctgga tcgcgatgtc
    13321 cgcggcgctg ccggtgaccc cgcccagtgg ctgatgcatc aggatccggg catgtggaag
    13381 cgcgaagcgc ttgcccttgg tgcccgccgc cagcaggaac tgagccatcg acgccgccat
    13441 tcccattcca taggtcgcga tatcgcacgg cgcgagcacc atcgtgtcgt agaccgccat
    13501 gcccgccgaa atcgagccgc ccggcgagtt gatgtacagg ttgatgtcct tggtgggatc
    13561 ctcggcggcc agcagcagaa tctgcgcgca cagcttgttc gcgatgtcgt cgtcgacctg
    13621 ggaacccagg aagatgatgc gctcggacaa cagtcgctca tacaccgagt cgacaaggtt
    13681 gaggccctgc gcggcgccac gcatgtcagt cacgactgga tacctgcttt ctgttaaggc
    13741 ttggggacgg cgcggtgtcc gtctccaaat gacactaacc aacctgcgcc gtcgcgcact
    13801 ccctggacgg cgcccgttcg ctcacagcgt cacttggcgt cggcagtcac ctcgtcggag
    13861 gtcgtttcct cggcagcttc tgcagcttcg gcggcctgct cacccgacgg gccgaagaac
    13921 tccgtggtgt cgatcacgtt gccgtcggtg tcactgacgg tggcaccgtg caccaccgcc
    13981 gcgacggtca ggccgcgacg cacgtcagcg aacatcgccg gcagctggtt gttctgctgc
    14041 aggaactgca gcagctgctg cggttccatc ccgtactgct gcgacatcag cacgagccgc
    14101 tcggtcaggt cgttctggcc gacctggatg tcgagcttgt cggcgacggc gtccatcagg
    14161 agctgggtct tgacggcctt ctccgcgttg gtgcggttgt ccgtgtcgaa ttcttcgcgg
    14221 ctgctgccct gctccttgag cgtgtcctcg aagcgcttct cgtcgtggtc gaggccgtgg
    14281 atggcgttgt gcaaggtgtc gtccacctgg gcctgaacga tgttctccgg cagcgggaca
    14341 tccgtctgct cgagcagcac ctccagcgcc ttatcgcgga tctgctccgc ctgctggatg
    14401 cgcttgacgc gctgcacctg ctcggtgagg ctgctcttca gctcgtcgat cgtgtcgaat
    14461 tcgcttgcca attgggcgaa ttcgtcgtcg ggctcgggca gctcgcgctc cttcaccgac
    14521 ttgacggtga cggtcacctg cgcctcctgg ccggcgtgct caccggcggc cagcgtggtg
    14581 gtgaagacac gggtctcccc ctccttcagg ccgacgatcg cctcgtcgag accgtcgatc
    14641 agctgaccgg agccgatctc gtgcgacagc ccctcggtct tcgcgtcggg cacgtcctcg
    14701 ccgtcgacgg tggccgacag gtcgatcgag acgaagtcgc cgttctcggc ggcacgctcg
    14761 acacccttga gcgtgccgaa gcgggcgcgc agattctgaa gctcggtgtc gacctcctcg
    14821 tcgttcacct cgatggggtc gacggtgatc ttcagcccct ccagctcggg gatctcgatc
    14881 tcgggccgga cgtcgacctc cgcggtgaag gtcaggtctt ggccgtactc cttgttggtg
    14941 atgtcgatct ctggctgacc gagtggccgc acgtcggagg tcgtcaccgc ttcggtgtag
    15001 cgggccggca ccgcgtcgcc gatgacctga tcgaacatcg cctgcttgtc gacgcgggcc
    15061 tcgagtagct tcaccggcac cttgccggga cggaagccgg gcatccggat ctgcttcgcc
    15121 atctgcttga aggccgtgtc gatgtcgggt tgcagctcag tgaagggcac ctccacgttg
    15181 atgcgaaccc gggtggggct caacttctcg acggtgctct tcacgctcat gctccttgtg
    15241 tgtgtcgtgt atgcgtctgg agtcggggtg acaggatttg aacctgcggc cttccgctcc
    15301 caaagcggat gcgctaccaa gctgcgctac accccggtct gcagctcgtc gctgcgccgt
    15361 gaccaccggc gatattacgg cctgacaccg caaagccctt aattggattt gattgatggg
    15421 gcgccggtag catctgctct tgctgcacac gcgggcgtag ctcaatggta gagccctagt
    15481 cttccaaact agctacgcgg gttcgattcc cgtcgcccgc tctgaacggg gcctttccag
    15541 gtcgcccggt gtcttaccag actcgtaccc agtcgacgag catgtccgcc gggtaggtcc
    15601 ccgcgctggg atcgccgccg cctgacccag ccaccgcaag gttcaagatc ggaaacagcg
    15661 tgtaaccggg ttcgttgaag tgccagtccg ggagcgagtt cgccgggacg tcgaagtacg
    15721 gttgcgcgcc gtcgacgtag tccttccaga agcgcatacc ggcgttatcc cattgcacgc
    15781 gccacgtgtg ccagccgctg tccaccgtga ttccgtgcga gacgtgttct ccgccgttca
    15841 acagtgcgtg gacggtggtg cccgcagccc agtgaccgtt gccgtaccac tccatgaggt
    15901 cgacttcgcc gccgttgacg gggttgttat tggccaagta ccaggcgggc caagaaccgg
    15961 gagtgaggca gttcagcttg attcgggctt cccaggtggt gccgataggt cctctgtact
    16021 tgccgaagag cttgccgctg tagtaggtgt tgccgtcctt ggcggcgcgg aagacgaggt
    16081 tggacttgcc gtcgaggaag acgtttcgcc gatcatcacg atattggccg acgttctcgg
    16141 gcagctccca aaacgttggg tcttccatat cctcgcgggc cagcgcgggc tcccatttcg
    16201 agtaatcggg ggctgagccc gccgggccgt cgaactcatc ccggaacagg tacgtctggg
    16261 ccgcaccacc tggcggcgcg gcgggtgccg gaccacgacc cgccgaggct cg
//
LOCUS       DASRJA010000504         4054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3010687,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000504 DASRJA010000000
VERSION     DASRJA010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3010687"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFC01_22095"
     CDS             complement(<1..737)
                     /locus_tag="VFC01_22095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN82348.1"
                     /translation="MSTVTTTDSADFYALEGLLDDDEQEYLHCVRDFMKSEVEPTVNQ
                     HWQRGSFPFEIVPGFRQLGLAGLPYQGFGCSGRSFLLDGMVAMELARTDPSIATFSGV
                     HGGLSMGSIYLCGSEEQKQRYLPSMARLEKIGAFGLTEPDVGSGASRGLMTTARRDGD
                     SWILNGQKKWIGNATFGDLTVIWAKDVDDGQVKGFVVANDSPGFSTTKLEDKIALRIV
                     QNAVITLDDVRVSEDDRLQNANSFRDTA"
     gene            complement(749..1576)
                     /locus_tag="VFC01_22100"
     CDS             complement(749..1576)
                     /locus_tag="VFC01_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN82349.1"
                     /translation="MTTASSVRLSVESLIPVLGQQIRVNVRKGSGVPLVLCNGVGASL
                     EVLDPLVEHLDPDTTVVRFDVPGTGGSPDSPLPYGFPYLAAVLGRLLNKLGLNGKVDV
                     LGLSWGGALAQQFAFQNPRRCRRLILVSTGTGALMVPGNPVVLAKMLTPRRFLDHDYA
                     STIAGDLYGGTARTDPSLVKQLFDRQLMAGSRVGYLHQLLAGAVWTSLFALPLIRQRT
                     LIVAGYDDPVVPVVNARIMARLLPHATVHMHAGGHVDLITNAAELGPVIESFRDQPD"
     gene            complement(1573..2025)
                     /locus_tag="VFC01_22105"
     CDS             complement(1573..2025)
                     /locus_tag="VFC01_22105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZN82350.1"
                     /translation="MTTVLEEPADLLKLVGQRLGTTEWIQITQDQVDRFADATGDKQW
                     IHTDPRRAAMGPFKGTIAHGYLTLSLAPVVISQVLQIRELTAALNYGLNRVRFPTPLP
                     VGSQVRAAVTVMSAQQKTSGVESVFTLTFEIDGEDRPACVADVIVLYP"
     gene            complement(2044..3738)
                     /locus_tag="VFC01_22110"
     CDS             complement(2044..3738)
                     /locus_tag="VFC01_22110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN82351.1"
                     /translation="MVATTRANADELAAPLDLLLTSSAVGVADRLLPNVAWSRFALNL
                     AKQPRTVASRTASLGRELVAIAEGRSERAPAKGDRRFADPAWRANPLLHRTMQAYLAT
                     NSTVDQLFSDANLDWRDAERIRFVLDFLTEGLAPSNNPVLNPLGWKALIDTGGLSAVR
                     GLRHFVDDMMSAPRVPSMVDPDAFTVGKDIAATPGSVVFRCDEFELIQYTPQSDQVFS
                     APLLMVPPVINKFYIMDIAPGRSMIEYFLRQGIQVFAISWRNPTAEHRNWGFDTYGQA
                     ILNALDAVEKITQSDRTHLQASCSGGIIAAMTAAHLNATGQGQRLAGLTLMVTVLDQR
                     KAGFAAAAIDEEAANIAIALSARKGYLDGRSLAEVFAWLRPTDLVWRYWVNNYVEGKT
                     PAAFDVLYWNADTTRMAAALHRDMVTMGVHNSLATPGAVSMLGTPVDLNQLTTDAYVV
                     AGIADHISPWQACYRSARMLGLKNLRFVLSSSGHIASLVNPPGNPRASYRQGGVDEPD
                     PEFWVESAEQHTDSWWPDFVAWLGERSGKKKDAPQSLGGAGMGPLEPAPGTYVLDH"
     gene            complement(3741..>4054)
                     /locus_tag="VFC01_22115"
     CDS             complement(3741..>4054)
                     /locus_tag="VFC01_22115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZN82352.1"
                     /translation="KELIIRGGYNVYPREIEEALYEHPAVAAVAVIGIPHESLGEEVG
                     AAVVLKEGISADLEELRQFVKGRVAAYKYPRRIWFVDSLPTGPTGKLLRREVKPPPEE
                     A"
BASE COUNT          764 a         1326 c         1208 g          756 t
ORIGIN      
        1 gcggtgtcgc ggaacgagtt cgcgttctgc agccggtcgt cctcggaaac tctgacatcg
       61 tcgagcgtga tgacggcgtt ctggacgatg cgcagcgcaa tcttgtcctc cagtttcgtc
      121 gtcgagaatc ccggggagtc gttcgcgacg acgaatcctt tgacctgacc atcgtcgacg
      181 tctttcgccc agatcaccgt gaggtcgccg aaggtggcgt tgccgatcca cttcttctgc
      241 ccattgagga tccacgagtc gccgtccctg cgagccgtcg tcatcaaccc gcgcgacgcg
      301 ccagacccga cgtccggctc ggtgagacca aacgcgccga tcttctcgag ccgcgccatg
      361 ctcggcagat accgctgctt ctgctcctcg ctgccacaca gatagatcga acccatcgac
      421 aagccgccat ggacacccga gaatgtcgcg atcgacggat cggtgcgggc gagttccatc
      481 gcgaccatgc cgtcgagaag gaacgacctt ccggaacaac cgaatccctg atacggcagt
      541 ccagccaatc cgagttgccg gaatcccggc acgatctcaa acggaaagct cccccgctgc
      601 caatgctggt tcaccgtcgg ctcgacctca gatttcatga agtcgcgcac gcagtgcagg
      661 tactcctgct cgtcgtcgtc gagcagtcct tccagtgcgt agaaatcggc gctgtcggta
      721 gtggtcacgg tgctcatgag cgcctccctc agtccggttg atctctgaaa ctctcgatca
      781 ccgggcccaa ttcggcggca ttggtgatca ggtcgacgtg gccgccggcg tgcatatgca
      841 cagtcgcgtg gggtagcaat cgcgccatga tcctggcgtt caccactggt acgacgggat
      901 cgtcgtatcc tgcaacgatc aacgtccgtt ggcggatcag cggtaaggcg aacaggctgg
      961 tccacaccgc ccccgcaaga agttgatgca ggtagcccac ccgcgaaccg gccatcagtt
     1021 ggcggtcgaa caactgcttg acgagggacg gatcggtgcg cgctgtgccg ccgtaaagat
     1081 cacccgcgat ggtcgatgcg tagtcgtgat ccaagaaccg gcgtggagtc agcatcttcg
     1141 ccagcacaac cggatttcca ggcaccatga gtgcacctgt gccggtggac accaggatca
     1201 accgccggca gcggcgcggg ttctgaaacg cgaactgctg ggccagcgcg ccgccccatg
     1261 acaaaccgag aacatccact ttgccgttga gcccaagctt attgagcagt cggccaagca
     1321 cggccgccaa atacgggaac ccgtacggca gcggcgaatc cggtgaacca ccagtgccag
     1381 gaacatcgaa ccggacgact gtcgtgtcgg gatccagatg ctccacgagc ggatcgagca
     1441 cttccagact ggctccgacg ccgttgcaca acaccagcgg cacgccgcta cccttgcgta
     1501 cgtttacccg gatctgttgg ccgagcaccg gtatcaggct ttccacgctc aacctcacag
     1561 aagaggcggt cgtcatgggt acaacacgat cacatcagcg acgcaggcgg ggcggtcttc
     1621 gccgtcgatc tcaaatgtca gtgtgaacac cgactcgaca ccggatgtct tctgctgtgc
     1681 gctcatcacc gtgacggcag cgcgtacctg tgacccgacg ggtagcggcg tcggaaaccg
     1741 caccctgttc agcccgtagt tcaacgcggc agtcagctcg cggatctgca gcacctgcga
     1801 aatcaccacc ggtgcaagcg acaacgtgag atagccatgc gcgatcgttc ccttgaacgg
     1861 acccatcgcg gcccgccgcg gatcggtatg aatccactgc ttgtctccgg tggcgtcggc
     1921 aaaacggtcg acctggtcct gggttatctg tatccactca gtggtgccaa gcctctgccc
     1981 gaccagcttg agcaggtcgg ctggctcttc gagaacagtt gtcatacgag gcctctcgcg
     2041 tggtcagtgg tccagcacat aggtgccggg ggcaggttcc agcggcccca tgcccgcgcc
     2101 acccagcgac tggggagcat ccttcttctt gccgctgcgt tcaccgagcc aagcaacgaa
     2161 gtccggccac caggaatcgg tatgctgctc ggccgactcc acccaaaact ccggatctgg
     2221 ctcatccacg ccgccctgcc gatagctcgc cctcgggttg ccgggcggat ttaccagcga
     2281 agcgatgtgc ccgctggagg acaacacgaa gcggaggttt ttgagaccga gcatccgggc
     2341 gctgcgatag caggcctgcc acggcgatat gtggtcggcg atgcccgcga caacgtaggc
     2401 gtccgtggtc aattggttga ggtccaccgg cgtgccaagc atgctgacag cgcccggtgt
     2461 ggcaagcgaa ttgtgcacac ccatcgtcac catgtcgcgg tgcagcgcgg cggccatccg
     2521 tgtggtgtcg gcgttccagt acaggacatc gaaggccgcg ggcgtcttgc cctcgacgta
     2581 gttgttcacc cagtaccgcc ataccaggtc ggttggccgc agccaggcga acacctccgc
     2641 cagcgatcgg ccgtccagat atcccttgcg ggccgacaag gcgatggcga tgttcgcggc
     2701 ttcctcgtcg atggccgcgg ccgcgaaccc ggccttcctc tgatcaagca ccgtgaccat
     2761 gagtgtcaac cctgcgagcc tttggccctg acccgttgca ttcagatgcg ccgcggtcat
     2821 cgcggcgatg atccccccgg agcaggatgc ctgcagatgc gtgcgatcgg actgcgtgat
     2881 cttctcgacg gcgtccagtg cgttcagaat tgcctgaccg taagtgtcga aaccccaatt
     2941 cctgtgttcc gcagtaggat tgcgccacga tatcgcgaag acctggatgc cctgccgcag
     3001 aaagtactcg atcatgctgc gcccgggcgc gatatccatg atgtagaact tgttgatcac
     3061 cggtggcacc atcagcagcg gcgctgagaa gacctggtcg gactgcgggg tgtactggat
     3121 cagctcgaat tcgtcgcacc ggaagaccac cgaacccggc gtggcggcga tgtcctttcc
     3181 caccgtgaac gcgtcgggat ccaccatcga cggcactcgt ggggccgaca tcatgtcatc
     3241 gacgaagtgg cgcaaccctc gcacagcgct gagtccaccg gtgtcgatga gggccttcca
     3301 ccccaacggg ttcaacaccg ggttgttgct cggcgcgagt ccttcggtca ggaaatccaa
     3361 aacgaatcga atcctctcag cgtcccgcca atcgagattc gcgtccgaaa agagttgatc
     3421 cacagtgctg ttcgttgcca gataagcctg catcgtgcgg tggagcagcg gattggcacg
     3481 ccacgccgga tccgcgaatc tcctgtcgcc cttcgcgggg gcccgctccg agcggccttc
     3541 ggcaatcgcg accagttccc gaccgagtga cgctgtgcgc gacgccaccg tgcgcggttg
     3601 cttggcgagg ttcaacgcga atctgctcca cgccacgttg ggcaacagcc gatccgccac
     3661 gccgaccgcc gaactcgtca acagaagatc cagcggcgct gcaagctcgt cggcgtttgc
     3721 tctggtggtg gctaccacgg ttatgcctcc tcgggtggcg gtttcacttc acgacgcagc
     3781 agctttcccg tcggaccggt gggcaggctg tccacgaacc agatccggcg tgggtacttg
     3841 tatgcggcga cccgaccctt gacgaactgt cgaagttcct ccaggtcggc ggatatgccc
     3901 tccttcagaa ccaccgctgc gccgacttcc tcgcccagtg actcgtgggg aatgccgata
     3961 accgcaaccg cggctaccgc gggatgctcg tacagcgcct cttcgatctc gcgcgggtag
     4021 acgttgtatc cgccgcggat gatgagctct ttct
//
LOCUS       DASRJA010000505         5320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3055803,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000505 DASRJA010000000
VERSION     DASRJA010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3055803"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFC01_22120"
     CDS             <1..308
                     /locus_tag="VFC01_22120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82353.1"
                     /translation="FVVGSQLKRIATNLEECAELVRTIKSHAEAIEPGVEHINRTGGV
                     VAGALPLLYGMAEGIVVGVTPRPTEPEVREPAKPASGRRRSRLHEGVGYVPVGAPES"
     gene            complement(278..742)
                     /locus_tag="VFC01_22125"
     CDS             complement(278..742)
                     /locus_tag="VFC01_22125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82354.1"
                     /translation="MIRLGSLAGYAFEGPRLLGGWSPPSHPAVYAILYKPHPETKPET
                     YAVIYVGHADDLSTERLPFHHPRAHCWIRRAGSRWRVYICTYQVPGGLRSHREQITQE
                     LSAIYHPSCNEQQYERSWKDEWIGEYSAPTTGPLTTGREPTSGYDSGAPTGT"
     gene            complement(739..1113)
                     /locus_tag="VFC01_22130"
     CDS             complement(739..1113)
                     /locus_tag="VFC01_22130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82355.1"
                     /translation="MKWSVSVTAQGDRVVSHDEVLELADAVAAAGGIASGIGTTSFGA
                     QLIVEADDRGAAVQRGTAEFVRAAAQAGLPAWPIAKVDAISEADELGDLSFQQRVGPD
                     RSAPGPTRAQSTAAPERSDNQE"
     gene            complement(1125..2255)
                     /locus_tag="VFC01_22135"
     CDS             complement(1125..2255)
                     /locus_tag="VFC01_22135"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN82356.1"
                     /translation="MTEGAPFVSVLVAFGDQLRAAGLTVGSGDVLTYSAALARLDPTD
                     LLDLYWAGRTTIVTRRDDIAVYDRVFKKFFLDEGDALPEPLRLTVHNAADVQSTLVVP
                     ATEPSPEGGDEQPARLGLVASDAEVLRNKAFTACTAQELAALRRITARMRLNPPRRRT
                     RRTAASRRGRTPDPRRTVRETMRTHGEPLRLFWRCRRLRVRPLILIVDISGSMADYSR
                     GLLQFAHSAKRADTRVETFCFGTRLTRITKVLRRRRVDDAMEHAAKAVFDWEGGTRIG
                     DSLAAFVRDWGRRGMCRGGIVVICSDGLDRGDPQSLASAMERLSRLCHRVVWLNPHRG
                     NSRDFRPSTLGMMVASPYIDLLLPGNNLHDLEVFAAMLPALS"
     gene            complement(2248..3123)
                     /locus_tag="VFC01_22140"
     CDS             complement(2248..3123)
                     /locus_tag="VFC01_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010594155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN82357.1"
                     /translation="MDRPETVAQLTQLLREDGYLADRGLATALLISFSLGRPILIEGE
                     VGVGKTEVAKALASVFGRQLIRLQCYEGIDTHQALYEWDYARQMLQIRALSERQLANG
                     EAVDQLFGPKFLLERPLLQALRAGDQAVLLIDEIDRADDEFEAFLLEMLADFQITIPE
                     LGTIKADTSPLVVLTSNRTRELHDALKRRCIYHWVGYPSIEREVEIVLVRQPEVSQAL
                     TRKVVTAVNRLRALELAKPPGVAETIDWVRALDVIGATELTAETVADTLGAVVKEHDD
                     LELVRASLDGIVSGD"
     gene            complement(3159..3419)
                     /locus_tag="VFC01_22145"
     CDS             complement(3159..3419)
                     /locus_tag="VFC01_22145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82358.1"
                     /translation="MADEPEETVDVLRAIWAGTGQRVNDVDEACGPQNNCKNMKDVTF
                     DGLAEPGTDLGAAWRERLRREGKLSTSAASIREIVLGPAETQ"
     gene            3881..4366
                     /locus_tag="VFC01_22150"
     CDS             3881..4366
                     /locus_tag="VFC01_22150"
                     /inference="COORDINATES: protein motif:HMM:NF018099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HZN82359.1"
                     /translation="MDCRSDSATTGEGRFRVNANGKLLDVWLLVRCVSCDRTSKLTVH
                     ERAPVKSFDPAELHRYHVNDPELVTSRLLDPLLARRSRFTLDWTGAWQLDTSSAWLDE
                     AWPVQVEVVFEDPIPVRPERLIAQGLGLSRHEVLRRIKCDIPLRRPRSTGFTFTVMAG
                     G"
     gene            4483..>5320
                     /locus_tag="VFC01_22155"
     CDS             4483..>5320
                     /locus_tag="VFC01_22155"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005183837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZN82360.1"
                     /translation="MGLTFDHHTHGQRVLFATGAAVDNTIVAVTGLGAQRVLLIADRF
                     AVQIADAIAARAPVVARIHDIVQHVPIERARAAVATAKDNAADTVITIGGGSSTGLAK
                     IVARDTGLPIIAIPTTFAGSEATDVWGLTEAQRKTTGVDPHVLPKIVVYDASLSASMP
                     DQLAVSSGLNAVAHAIDGFWAPRADPINRALGTEGLRALIPGLRALHANPDDLAAREQ
                     VTYGAYLAAVAFSSAGSGMHHKICHALGGTYNLPHAETHAIVLGYVVSFNGPAAPDAQ
                     LRI"
BASE COUNT          900 a         1829 c         1665 g          926 t
ORIGIN      
        1 tgttcgtggt cggcagccag cttaaacgga tcgccaccaa cctcgaggag tgcgccgaac
       61 tcgtgcgcac catcaagagc cacgcggagg ccatcgagcc cggcgtggag cacatcaacc
      121 ggaccggtgg ggttgtcgcc ggcgcattgc cactgcttta cggcatggcc gaaggaattg
      181 tcgtcggcgt cacgccgcgc ccgaccgaac ccgaggtgcg cgagccggcc aagccggcct
      241 ccgggcgccg tcgctcacgg ctgcatgaag gggtcggcta cgtcccggtg ggcgctccgg
      301 agtcgtaacc gctggtgggc tcacgtccgg tggtcagcgg tccggttgtc ggcgctgagt
      361 attcgccgat ccattcgtcc ttccaggacc gctcgtactg ctgctcgttg caggacgggt
      421 ggtagatcgc gctcagttcc tgggtgatct gttcgcggtg cgagcgcagc ccgccgggta
      481 cctggtaggt gcagatgtag acgcgccacc gactgcccgc gcgacggatc cagcagtgcg
      541 cccgtggatg gtgaaacgga agccgttctg tggacagatc gtcggcgtgg ccgacgtaga
      601 tcaccgcgta ggtctcgggt ttggtctccg gatgcggctt gtacaagatc gcgtacaccg
      661 ctggatgtga cggcggtgac cagccgccga gcagtcgagg accctcgaag gcatagccgg
      721 caagcgaacc gagcctgatc attcttggtt gtcactccgt tcaggcgcag ccgtgctttg
      781 ggcgcgggtg gggccgggcg cggagcgatc cgggcctacc cgctgctgga atgagaggtc
      841 gccaagttcg tcggcctcgc tgatggcatc aaccttagcg atcggccagg ccggcaaccc
      901 ggcttgtgcc gcggcccgga cgaactcggc agtaccgcgt tgcaccgcgg caccccgatc
      961 gtcggcttcc acgatcagtt gtgcgccaaa actggtggtg ccgatgccag aggcgatccc
     1021 accggccgcc gccaccgcgt cggcgagttc cagcacctcg tcatggctca cgacgcgatc
     1081 accctgcgcc gtcaccgaca cactccactt catcgattcg ctcctcaact cagcgctggc
     1141 agcatcgccg cgaacacttc caggtcatgc aggttgttgc cgggtagcaa cagatcgatg
     1201 tacggggagg cgaccatcat gccaagggtg cttggtcgaa agtcgcggct gttgcctcgg
     1261 tgcgggttca accacaccac ccgatggcac agccggctca gccgctccat cgcgctcgcc
     1321 agggattgcg ggtcgccccg atccagtccg tccgaacaga tcaccacgat gccgccgcgg
     1381 cacatgcccc gccgtcccca gtcacgcacg aacgcggcca gcgagtcccc gatccgagtg
     1441 ccaccctccc agtcaaaaac agccttggcg gcatgctcca tcgcatcgtc gacgcggcgt
     1501 cgccgcaaca ccttggtgat gcgagtcagc cgggtgccga agcagaaagt ctccactcga
     1561 gtgtcggctc gctttgctga atgggcgaac tgcagcagac cacgggagta gtcggccatc
     1621 gatcctgaga tgtcaacgat caggataagc ggtcgcaccc gcagccgacg gcaacgccag
     1681 aacaacctca agggctcgcc gtgggtccgc atcgtttcgc gcaccgtacg acgaggatcc
     1741 ggcgttcggc cccgccgcga cgcagccgtg cggcgggtcc tacggcgcgg cggatttaag
     1801 cgcatgcgcg cggtgatccg gcgcagcgcc gccagctcct gtgccgtaca cgccgtgaaa
     1861 gccttgtttc gcaacacttc tgcgtcagaa gcgaccagcc ccagccgcgc cggttgctcg
     1921 tcgccgcctt cggggctggg ttcggtcgcg ggcacgacca gcgtcgactg cacgtcggcc
     1981 gcgttatgga cggtgagccg caacggttcc ggcagcgcat cgccttcgtc gaggaagaac
     2041 ttcttgaaca cccggtcata gaccgcaatg tcgtcgcgtc gggtgacgat ggtcgtgcgt
     2101 ccggcccagt aaagatccag caggtcggtc ggatcgagtc gagccagcgc ggcgctgtag
     2161 gtgagcacgt caccagatcc gaccgtcaga cccgcggcac gcaattgatc gccgaacgcg
     2221 accagcacgc tgacgaacgg cgccccctca gtcacctgag acgatcccat ccaggcttgc
     2281 ccgcaccagt tccaggtcgt cgtgctcctt cacgaccgcc ccgagcgtgt cggccaccgt
     2341 ctcggcggtc agctcggttg ccccgatgac gtccagagcc cgcacccagt cgatcgtctc
     2401 ggcgacgcct ggcggcttgg ccagttccag ggctcgcagg cggttgacgg cggtaacaac
     2461 cttgcgggtc aaggcttgcg acacctcggg ttgccggacg agaacgatct ccacctcgcg
     2521 ctcgatgctc ggatagccga cccagtggta tatgcatcgg cgcttgagcg cgtcgtgtag
     2581 ttcacgggtg cgattcgacg tcaacacgac cagtggcgac gtgtcggcct tgatcgtccc
     2641 tagctcggga atggtgatct ggaagtcggc gagcatctcg agcaggaacg cctcaaactc
     2701 gtcgtcggct cgatcgatct cgtcgatgag caggactgcc tgatcaccgg cacgcagtgc
     2761 ctgcaacaac ggtcgctcaa ggaggaactt cgggccgaag agctggtcga ctgcctcgcc
     2821 gttggccagc tgacgctcgg acagcgcccg gatctgcagc atctgccggg cgtagtccca
     2881 ttcgtacaat gcctgatggg tgtcgatgcc ttcatagcac tgcagccgga tcagttgccg
     2941 gccgaagacg gacgcgagag cctttgccac ctccgtctta ccgacgccga cctcaccctc
     3001 gatcaggatt ggccgcccca gcgaaaagga gatcaaaagc gcggtggcca acccgcggtc
     3061 ggcaagatat ccgtcttcac gaaggagttg ggtcagctgg gccacggtct ccggacggtc
     3121 catgcagtct ctccctaacc gcccacgcgc cgttgacgct actgagtctc ggcggggcca
     3181 agcacgatct cgcgaatgct ggccgccgac gtgctgagct tgccttcccg tcgcagccgc
     3241 tcccgccacg ccgcgcccag gtcagtaccc ggctcggcca ggccgtcgaa ggtgacatcc
     3301 ttcatgttct tgcagttgtt ctgcggtccg caggcctcat caacgtcgtt tacccgttgc
     3361 ccggtaccag cccagatcgc gcgcagtacg tctacggtct cctccggctc gtctgccatg
     3421 gcccgccacc ctctccggtc ctttcgctca accacccacc ctaggccgcg caggcggcca
     3481 acgcgactac ggattaaggc ttcgcttaca cgacaccatc gccttcgcca ctgagggtcc
     3541 cgatagcact gacgaagtct cggtcgaaag ccgtttgacc cttccttccg cgttcccgta
     3601 ggttgccccc aggcccatcc cagtgaggac agaggacaaa ccgcgtgacc ccgcctgctc
     3661 ttttagtctg acgcctttct tccgctcacc tgtagccgac gcttccgtcg gttctgccgg
     3721 gctgcccttg tgcgcccggt catacagcgt ttgaggccgc gcgtgatcgg cctcgtgtca
     3781 ggtctagaga tcaggtctag agatcatgtc ttcacaacct cataccgtgc tgccctgggt
     3841 cgttcgtcgg acaaggctgc ctctgttgtc gttgcggtgc gtggactgcc ggtcggactc
     3901 ggccaccacc ggcgagggca ggttccgcgt caacgccaac ggcaagctac tggatgtgtg
     3961 gctgctggtc cgctgcgtat cctgcgatcg gacgagcaag ctcaccgtgc acgagcgagc
     4021 gccggtcaaa tccttcgatc cggccgagct gcaccgctac cacgtcaacg atccggaact
     4081 ggtgacgtcc aggttgttgg atccgttgct cgcccggcgc agccgcttca ctctggactg
     4141 gacgggggcc tggcagctgg acacctcgtc ggcatggctc gacgaggcgt ggccggtcca
     4201 ggtggaggtc gtcttcgagg atccgatacc ggtgcgcccg gaacggctca tcgcgcaagg
     4261 gctcggcctt agcaggcacg aggtactgcg ccggatcaag tgcgacattc cgctgcgtcg
     4321 tccgaggagc accggattca cctttaccgt gatggccgga ggctagcggg gatgtagcca
     4381 acgttgtgcc cgctgcgcct cagggctcgg gatctccaga aacattggga tggcaaactg
     4441 gaataggcgt taccagcacc tgacgcaagg gatgggtaac ggatgggcct caccttcgac
     4501 catcacaccc acgggcagcg cgtgctgttc gcaacgggcg cggccgtgga caacaccatc
     4561 gtcgcggtga cggggctcgg cgcacagcgc gtgctgctca tcgctgaccg attcgcagtt
     4621 cagattgccg acgcaatcgc cgcacgcgca cctgtggtcg cccgcatcca cgacatcgtc
     4681 cagcacgtcc cgatcgagcg tgcccgcgcc gcggtggcca ccgcgaagga caacgcggcc
     4741 gacactgtga tcacgatcgg cggcggctcg tccaccggcc tggcgaaaat cgtcgcccgc
     4801 gataccggac ttccgatcat cgcgatcccg accacgttcg ccggctccga ggcgaccgac
     4861 gtgtggggcc tcaccgaggc gcagcgcaag accaccggag tcgaccccca tgttctgccg
     4921 aagatcgtcg tgtacgacgc atcgctgagc gcaagcatgc ccgaccagct cgcggtctct
     4981 tctggactca acgcggtcgc ccacgcgatc gacgggttct gggcaccccg cgccgacccg
     5041 atcaaccgcg ccctgggcac tgaagggctg cgcgcgttga tacctggctt gcgcgccctg
     5101 cacgcgaacc cggacgacct cgccgcacgc gagcaggtga cctacggcgc gtacctggcc
     5161 gccgtcgcgt tctcctccgc cggttccggc atgcatcaca agatctgcca cgcgctgggc
     5221 ggcacctaca acctgccgca cgccgaaacc cacgcgatcg tgctcggcta cgtggtcagc
     5281 ttcaacggcc ccgcggcacc ggacgcgcag ctgcgcatca
//
LOCUS       DASRJA010000506         9519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_995260,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000506 DASRJA010000000
VERSION     DASRJA010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995260"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VFC01_22160"
     CDS             <1..749
                     /locus_tag="VFC01_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZN82361.1"
                     /translation="AVAAARAGAAVQLVAALGTDSAADQLRAHLRDNEVGLDGVVSLP
                     GPSGSAVLIVDSSAENTIVVAPGANAHLTLTSADVRAVIADSDVVLLQLEIPIATAVA
                     AAGVARAAGAVVMLNASPTGAQPHQLLALSELADVVVVNEAEAREWHWPVPHLVITRG
                     ARGASYLGDDERFDVPAPAVEAVDTTGAGDVFAGVLAAGWLGGYEDALRRACAAGALS
                     TLVPGAGDCAPYAEAIDDAVSKRKAGERQI"
     gene            746..1567
                     /locus_tag="VFC01_22165"
     CDS             746..1567
                     /locus_tag="VFC01_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN82362.1"
                     /translation="MTTTTPRPPEGDWLGTPYLRFTREGPFGVCTLDRPEARNAMTPA
                     MYFGIRYAVRHVDADPDLAGLLITGTGDVFAPGGDMGGGDGSDNWLTFGSALGMDVTP
                     FETLRQSVKPVVSAVNGLCQGGGLQIALCSDMAVVSERATFRVPELLRGIADTYYSQM
                     LARLIGPVRTRDLMFTGRTLTAAEAQEWGMVARVVPHDELLDVAREVLGQCCRTAPAA
                     RSMVKSSLDNYLGLFDRIGMQASYSSHEALEGFRAFKERRSPDWVHPDLRIDGRL"
     gene            1669..2313
                     /locus_tag="VFC01_22170"
                     /pseudo
     CDS             1669..2313
                     /locus_tag="VFC01_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242062.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2523..3029
                     /locus_tag="VFC01_22175"
     CDS             2523..3029
                     /locus_tag="VFC01_22175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82363.1"
                     /translation="MVATYLARIAAGDKLTGRAPVEVEVALAEQRLAEAIAAYEAKLA
                     TRHRRGRRPVPVERYCRVRQAQAKLDKARAAEAARAAEAAEARADKRVANITDPQSRM
                     QPVRAQHRQDHQLPRRSTTARLIRRPAVPAPTRRISDDPNLQQPLSADFRAQIAKDNS
                     SVFSEYRI"
     gene            3045..3374
                     /locus_tag="VFC01_22180"
     CDS             3045..3374
                     /locus_tag="VFC01_22180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010594979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN82364.1"
                     /translation="MRTVTHSDALARFGYALSDATRTEILLTLREGPGYPSDLAEKIG
                     VSRQILSNHLACLRGCGLVVAQPEGRRSRYELADARIAHALDDLVGLVLAVDPACCPA
                     AETDACC"
     gene            3374..3985
                     /locus_tag="VFC01_22185"
     CDS             3374..3985
                     /locus_tag="VFC01_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009477300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZN82365.1"
                     /translation="MSAPIGTRRATLQRRIRLLVAATITYNVIEAVVALAEGTRVSSS
                     ALIGFGLDSVVEISSAAAVAWQFSAKDPETREKAALRFIAISFFALAVYVAVDAVLSL
                     LGLGEARPSLIGIVLAAASLVIMPVLSLTQRRTGRELGSLSAVADSKQTLLCTYLSAV
                     LLAGLVLNSLWGWSWADPVAALGIAAIAVREGVNAWRGDPCCP"
     gene            4006..4619
                     /locus_tag="VFC01_22190"
                     /pseudo
     CDS             4006..4619
                     /locus_tag="VFC01_22190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045637.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
     gene            4630..4806
                     /locus_tag="VFC01_22195"
     CDS             4630..4806
                     /locus_tag="VFC01_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82366.1"
                     /translation="MARKQMNSKQEAGWLWTPLIIGLIIGVTVAAMTDQWLWSTAGVL
                     GGAAFGWIGYARTR"
     gene            complement(4812..6854)
                     /locus_tag="VFC01_22200"
     CDS             complement(4812..6854)
                     /locus_tag="VFC01_22200"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HZN82367.1"
                     /translation="MDFYSAYRQGFVRVAACTHHTTLADPAANAESVLRLARDCHDDS
                     VALAVFPELTLSGYSIEDILMQDALLDAVEEALVEVVAASAVLLPVLVVGAPLRRGHR
                     IYNTAVVIHRGRVLGIVPKSYLPTYREFYERRQVAAGDDERGVIRLGGAEVPFGPDLL
                     FAAADMPGFVLHVEICEDMFVPIPPSAEAALAGATVLANLSGSPITVGRAEDRCLLAR
                     SASSRCLAAYVYAAAGEGESTTDLAWDGQTMIWENGVLLAQSERFPRGERRSVADVDL
                     ELLRSERLRMGTFDDNRRHHRVPAESFRRVDFQLDPPTGDIGLRRDVERFPFVPSDEA
                     RLEQDCYEAYNIQVAGLEQRLRALNYPKVVLGLSGGLDSTHALIVAARAMDREDRPRS
                     DILAFTLPGFATGERTKRNATRLAEALGITFETIDIKATAELMLKEMDHPFSRGEKVY
                     DVTFENVQAGLRTDYLFRLANQRGGIVLGTGDLSELALGWSTYGVGDQMSHYNVNGGV
                     PKTLIQHLIRWVITSQQFDDAVNEVLQSVLDTEISPELVPTGEDEEIQSSESKVGPYV
                     LQDFSLFQVLRFGFRPSKVAFLAWHAWSDPHRGDWPVGFPEHKRPAYSLKEIRHWLQV
                     FAQRFYSFSQFKRSALPNGPKVSHGGSLSPRGDWRAPSDMSARTWLDEIEREIPED"
     gene            6899..7438
                     /locus_tag="VFC01_22205"
     CDS             6899..7438
                     /locus_tag="VFC01_22205"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HZN82368.1"
                     /translation="MSDVAVLVIDMLNTYEHEDADVLAGNVADIIDPLTGLVSTARGR
                     EDVDLIYVNDNYGDFDADFRDVVDAALHGDRPDLVTPIAPEENSFRLLKVRHSAFYAS
                     SLDYLLRRLETRQLILTGQVTEQCILYTALDAYVRHFSIVVAPDAVAHIDPELRDAAL
                     AMMHKNMHAEIVPAEKCLG"
     gene            7553..7969
                     /locus_tag="VFC01_22210"
                     /pseudo
     CDS             7553..7969
                     /locus_tag="VFC01_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356340.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
     gene            8179..9015
                     /locus_tag="VFC01_22215"
     CDS             8179..9015
                     /locus_tag="VFC01_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HZN82369.1"
                     /translation="MDAPELVALLHGRRVAVLSGAGMSTDSGIPDYRGPDSPPSNPMT
                     IRQFTSDPAFRQRYWARNHLGWRHMDETLPNAGHRALAELEHAGVVSGVITQNVDLLH
                     TKAGSRTVVNLHGTYAQVICLDCGHTMSRAALADLLEAANPGFIERTEAVGGIAVAPD
                     ADAVVGDTTSFRIIDCPHCGGMLKPNIVYFGENVPKDRVDEAYSLVDRADALLVAGSS
                     LTVYSGYRFVRHAATLGIPIAIINRGHTRGDDLATVKVDGGCSPMLALLADELPAVAN
                     SA"
     gene            9024..9311
                     /locus_tag="VFC01_22220"
     CDS             9024..9311
                     /locus_tag="VFC01_22220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82370.1"
                     /translation="MPDEASWLQSLLETRRPDPGPVVGRWALGIGDMVADHPLTPDKL
                     HWLARKLNHFGGVAISEEDVEFDGDDVNWSDVTEIHTRSLIEYLFTGGVDT"
     gene            9354..9506
                     /locus_tag="VFC01_22225"
     CDS             9354..9506
                     /locus_tag="VFC01_22225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82371.1"
                     /translation="MIEAISRAAVRQCFEATAQAHGVDAILVRIRSLSGLLQNVAPDF
                     GDVAVS"
BASE COUNT         1607 a         3064 c         3211 g         1637 t
ORIGIN      
        1 aggcggtggc ggcggcccgc gcgggcgcgg ccgtgcaact tgtcgcggcg ctcggcaccg
       61 actcggcggc cgatcagttg cgcgcccacc tccgcgacaa cgaggtcggg ctggacggcg
      121 tggtcagcct gccagggccc agcggctcgg cggtgctgat cgtcgactcg tcggcggaaa
      181 acaccatcgt cgtggcccct ggcgcgaacg cccacctgac cctaacctcc gccgacgttc
      241 gcgctgtgat cgccgacagc gacgtcgtgc tgctgcaact cgagatcccg atcgcaacgg
      301 ccgtcgccgc ggcgggggtg gcgcgggccg cgggtgcggt ggtcatgctc aacgcatcgc
      361 cgacaggagc ccaaccccac cagttgttgg cgctgtccga gctggccgac gtggtggtgg
      421 tcaacgaggc cgaggcgcgg gaatggcact ggcccgtacc gcatctggtg atcacccgtg
      481 gtgcgcgcgg cgcaagttat ctcggcgacg acgaacgatt cgacgtgccc gcccctgccg
      541 tcgaggccgt cgacacgacg ggcgcaggcg atgtgttcgc cggggtgctg gccgctggct
      601 ggctgggcgg gtatgaggac gcgctgcgcc gcgcctgcgc ggccggtgcc ttgtcgacac
      661 tggtcccagg ggctggtgac tgcgcgccat acgctgaggc catcgacgac gctgtatcca
      721 aacgaaaggc tggggaaagg cagatatgac gacaaccacg ccacgtccac ccgaaggaga
      781 ctggctgggc acgccgtacc tgcgcttcac ccgcgagggc ccgttcgggg tgtgcacact
      841 ggaccggccg gaggcgcgca acgcgatgac gccggcgatg tacttcggca tccgctacgc
      901 cgtccggcac gtcgacgccg atcccgacct cgcgggcctg ctcatcaccg gcaccggtga
      961 cgtgttcgcg cccggcgggg acatgggcgg cggcgacggc tcggacaatt ggctgacgtt
     1021 cgggtccgcg ctcggcatgg acgtgacgcc gttcgagacg ctgcgccagt cggtcaagcc
     1081 ggtggtgtcg gcggtcaacg ggttgtgtca gggcggcggg ctgcagatcg cgctgtgcag
     1141 cgacatggcc gtggtgagcg aacgggcgac gttccgcgtc cccgaactgc tgcgcggcat
     1201 cgccgacacc tactacagcc agatgctggc ccggctgatc gggccggtgc gcacacgcga
     1261 cctgatgttc accgggcgga cgttgaccgc ggccgaggcg caggagtggg gcatggtcgc
     1321 gcgggtggtc ccgcacgatg agctgctcga cgtggcccgc gaggtgctcg ggcaatgctg
     1381 caggacggcg ccggcggcgc gcagcatggt gaagtcgagc ctcgacaact atctggggtt
     1441 gttcgaccgc atcggcatgc aggccagcta ctcctcgcat gaagcgctcg agggctttcg
     1501 cgcgttcaag gagcgccgct cgcccgactg ggtgcatccc gacctcagga tcgacggccg
     1561 actctagatc gatttgtgca cactttcacg cgctgagggg gctgtcgcga aatgacggcg
     1621 cgggtgtgag cgtgtcgtag tgatctgacc tggcgttgcg tcgaatatgt gttggtggcc
     1681 aagggttatc gaccggttgt gcgtgatcag cggtttttgt ttcctccgga tatgcgcgag
     1741 tggctgccgc cggatcatcc ggtgtggacg gttatcgcga tcgtggaccg gttggagacc
     1801 tcggcatttc accggaatcg acgtgtcggg ggtgtgggcc gggccggcta tgacccggac
     1861 atgctgttga cgttgctgat ctgggcctgg ttgtcgggcg tgcggtcctc gcgccggatc
     1921 gagcgggcgt gtgccgatgt ggtgtcgtat cgagtgatct gcgccggtga tgggcctgat
     1981 cacgcgacga tcgcgcggtt tcgcgccgac aaccatgggg cgtgccggca gttattcacg
     2041 caggtgttga tgatggccgc ggggctgggg tggggccggt tggagacggt cgctctggac
     2101 ggggtgaaga tcgcctcgaa cgcatcgctg gcggcgaatc gcaccgcatc ggggttggcg
     2161 aaagcggccg aagcggaggc ggcccgtatc gccgaggccg cggtgagcgc gcatgcggcc
     2221 accgacgccg aagaggacgc gcggttcggc gagtccaacc cggacttggt gccagccgag
     2281 ttggctgatc cggacgcgcg ggcggcgctc atcgatgcgg tgaagacaca gctggacacc
     2341 gacgccaccg gccccgacga tcggccaccg ccagacgatg ctgaaccacc agcgacccac
     2401 cacctggaac caaccgaatc ggttgagctg agccggttcc gcatgatcgg tcggcgcgca
     2461 tcgccctagc ccaagagcgt atcgaagccc agatcgccgc cgagcgagcc gcgcgcgagc
     2521 ccatggtggc cacgtatctg gcccggatcg ctgccgggga caagctgacc ggtcgggctc
     2581 cggtcgaggt ggaagtggca ttggccgagc agcggttggc cgaggcgatc gccgcctatg
     2641 aagccaagct cgcgacgcgg caccggcgtg gtcgccggcc ggtgccggtc gagcggtact
     2701 gccgagtgcg ccaggcccaa gccaagcttg acaaggcgcg cgccgccgaa gcggcccgcg
     2761 ccgccgaggc ggccgaggct cgggccgata agcgcgtggc gaacatcacc gacccgcagt
     2821 cgcggatgca gccggtgcgc gcacaacatc ggcaagatca tcagctacct cgccggagca
     2881 ccactgcccg cctgatcagg cgacctgcag taccggctcc aacccgtcga atcagcgacg
     2941 atcccaattt gcaacagccc ctgagcgcgg attttcgtgc acaaatcgca aaagacaata
     3001 gttcagtctt ttctgaatac agaatctgat gtactgtccg tctcatgcgg acagtgactc
     3061 atagcgatgc gctggcgcgg ttcggatacg cgctgtcgga cgccacccga accgagatcc
     3121 tgctgacact gcgcgagggg ccgggctatc cgtccgacct cgccgagaaa atcggggtgt
     3181 cgcggcagat cctgtccaac cacttggcgt gcctgcgcgg ctgcggcctg gtggtggcgc
     3241 agcccgaggg ccgccgcagc cgctacgagc tggccgacgc gcggatcgcg catgcactcg
     3301 acgacctcgt cggcctggtg ctggccgtcg acccggcctg ctgccccgcc gccgagaccg
     3361 acgcctgctg ctgatgtccg ccccaatcgg gacgcgacgc gcgacattgc aacgccgcat
     3421 ccggctgctg gtcgccgcga ccatcaccta caacgtgatc gaggcggtgg tcgcgttggc
     3481 ggagggcacg cgtgtctcgt cgtccgcgtt gatcggcttc ggcctcgact cggtcgtcga
     3541 gatctcgtcg gccgcggccg tggcgtggca gttctccgct aaggaccctg agacgcggga
     3601 gaaggccgca ctgcggttca tcgcgatctc gttcttcgcg cttgcggtgt acgtcgcggt
     3661 cgacgccgtg ctctcgctac tcggcctcgg cgaggcgcgg ccgtcgctga tcggcatcgt
     3721 gctcgcggcg gcaagcctcg tcatcatgcc ggtgctgtca ttgacacaac gccgcaccgg
     3781 ccgcgagctg ggatcgttgt ccgccgttgc cgattccaag cagacgctgc tgtgtacgta
     3841 cctgtccgcg gttctgctgg cgggcctggt gctcaacagc ctgtggggct ggtcgtgggc
     3901 cgaccctgtg gccgcactcg gcatcgcggc gatcgcggtg cgagaaggcg tcaacgcgtg
     3961 gcgcggagac ccgtgttgcc cgtgagagtc aaccagggag tcatcatgcc gatcgttgca
     4021 ctcgtattat tcgcgagctt cgctgcgctc ggattcggtt ggcgcagttg ggaacagcga
     4081 cgccggaccg gatcagctgg cttccgtgga atcagcgggg agttgggttc gacgaatggt
     4141 ttgccggagt aggcttcgtc gctgcactgt tggccgccgt gctggccccc gtgttacagc
     4201 tgctcggtgt ggtgtcgcag gcggccgttc tgcacgctgc gtggatccag atcaccggca
     4261 tcgcgatcgc ggcgctcggc atcttcgcga cggtgtacgc gcaactcgac atgggtgatt
     4321 cgtggcggat cgctgttgat cccagcgaga cgacgacgct cgtccgcacc ggcgtgttcg
     4381 gttgggttcg caacccgatc ttcaccgcga tgatcacgtt tggcttcgga atcgctttgg
     4441 tgacaccgaa tctggtggca atagcgggct tcgtcttgct ggtggcgacg atcgagcttc
     4501 aggtgcgcgt ggtcgaagag ccgtatttgc tgaccgtgca cggcgacgcg taccgcgact
     4561 atctggccac cgtcggtcgt ttcgtccctg ggttgggcct cgaccgctcg ccgcgctagc
     4621 gtgggtcaga tggcacgaaa gcagatgaac agcaagcagg aagcgggctg gctgtggacg
     4681 ccgttgatca tcgggttgat catcggcgtc accgtcgcag cgatgaccga tcaatggttg
     4741 tggtcaaccg ccggagtgct cggtggagcg gcgttcggct ggataggtta cgcaagaacg
     4801 cgctaacagc gttaatcctc cgggatttcg cgctcgatct cgtcgagcca agtgcgggca
     4861 gacatgtccg acggtgcccg ccaatcaccg cgcggcgaca acgatccacc atgtgacacc
     4921 ttcggcccgt tcggcaaggc cgaacgcttg aactggctga acgaatagaa ccgctgcgcg
     4981 aacacctgta gccagtgccg aatctccttc agcgagtagg ccggccgctt gtgctcgggg
     5041 aagccgacgg gccagtcgcc gcgatgcggg tcgctccatg catgccatgc caaaaatgcg
     5101 accttggacg gccggaaccc gaagcgcagt acctggaaca gcgaaaaatc ttgcagcaca
     5161 tacggcccca ccttggactc actgctctgg atctcctcat cctcgcccgt cgggaccagt
     5221 tcggggctga tctcggtgtc gaggaccgac tgcaacacct cgttgaccgc gtcatcgaac
     5281 tgttgggagg taatcaccca gcggatcaaa tgctggataa gcgtcttagg cacgccgcca
     5341 ttgacgttgt agtgcgacat ctggtcgccc acaccgtatg tggaccaccc aagcgccaat
     5401 tcggacaaat cgcccgtgcc cagcacgatg cccccgcgct ggttcgcgag ccggaacagg
     5461 tagtccgtac gcaaccccgc ctgcacgttc tcgaacgtga cgtcatagac tttctcaccg
     5521 cgcgagaacg gatgatccat ctccttgagc atcaactcgg cggtggcctt gatgtcgatg
     5581 gtctcgaacg ttatgcccag cgcctcggcg agcctggtgg cgttgcgctt ggtgcgttcg
     5641 ccggtagcga accccggcag cgtgaacgca agaatgtcgc tgcgcggccg gtcttcccgg
     5701 tccatcgcac gggccgcgac gatcaacgcg tgcgtggagt ccaagccgcc ggacagtccg
     5761 agaaccacct tcgggtagtt cagggcgcgc agccgttgct ccagacccgc tacctgaatg
     5821 ttgtaggcct cgtagcaatc ctgttccagc cgagcctcat ccgacggcac gaacgggaat
     5881 cgctcgacat cgcgacgcaa gccgatgtca cccgtcggcg ggtcgagttg aaaatccacg
     5941 cgccgaaacg attccgcggg tacgcggtgg tggcgtcggt tgtcgtcgaa ggtgcccatc
     6001 cgcagccgtt ccgagcgaag cagctcgaga tccacgtcgg cgaccgagcg ccgctcccct
     6061 ctggggaacc gctcagattg cgcgagcaac acgccgttct cccaaatcat cgtctggccg
     6121 tcccacgcca gatcggtggt ggactcccct tcgccggccg cggcgtagac gtacgcggcc
     6181 aggcagcgtg acgacgccga tcgcgccagc agacaacggt cttcggcgcg gccgacggtg
     6241 atcggactgc ccgaaaggtt ggcaagcacc gtcgcgcccg cgagtgcagc ctccgcgctc
     6301 ggcgggatcg gcacgaacat gtcctcgcag atttcgacgt gcagcacgaa tcccggcata
     6361 tccgcggcgg cgaacagcag gtcgggcccg aacggcacct ctgcgccgcc gagccggatc
     6421 acacctcgct cgtcatcacc cgcggcaacc tgacggcgct cgtagaactc ccggtacgtc
     6481 ggcagatacg acttcgggac gatgcccagc accctgccgc ggtggatgac cacggcggtg
     6541 ttgtagatgc ggtgaccgcg gcgcagcggc gcgccgacga ccagcacggg caggagcacc
     6601 gccgacgccg cgacaacctc gaccagagcc tcctcgaccg cgtcgagcag cgcatcctgc
     6661 atcaggatgt cctcgatgga atagccggac agcgtcaact ccggaaaaac cgcgagcgcg
     6721 acgctgtcgt cgtggcagtc acgggccagc cgcagcaccg actcggcgtt ggccgcgggg
     6781 tccgcgaggg tcgtgtggtg ggtgcacgcg gccacccgca cgaagccctg gcggtacgcc
     6841 gagtagaaat ccatgcccta attgttgccc gaaagtgccg ctgggtatgc aagaccctat
     6901 gagtgacgtt gctgtgctgg tcatagacat gctgaacaca tatgagcacg aagacgcgga
     6961 cgtgctcgcg ggcaacgtcg ccgacatcat cgatccgttg acggggctgg tatccacggc
     7021 tcgcggacgc gaggacgtcg acctgatcta cgtcaacgac aactacggcg acttcgacgc
     7081 cgacttccgc gacgttgtcg acgcggcgct gcacggcgat cggcccgatc tggtgacgcc
     7141 catagcgccc gaggaaaaca gcttcagact gctgaaagtg cggcatagcg cgttctacgc
     7201 cagctcgctg gactacctgc tccgccgact cgaaacccgt caactgatcc tcaccggaca
     7261 ggtcaccgag caatgcatcc tctataccgc gctggatgcc tatgtgcggc acttctctat
     7321 cgtcgtcgca ccggacgccg tcgcgcacat cgatcccgaa ctccgcgatg ccgcgctggc
     7381 gatgatgcac aagaacatgc acgccgagat cgtgcccgcc gagaagtgcc tgggctgagg
     7441 cccgacttgg cctcgtgttg gcttgcagtt cgcccgcacg caccgctgac gatccgacga
     7501 ttttgattgc gtcgcgcatc gtgaatcaaa cgacccaacc ccggaggaaa tcgtgaataa
     7561 gcctgccgtt caacgtcagt cgcgtccctt gctgcccgag ttgtccgaac tcttcaacgc
     7621 gttcccgacg ttcgctaacc tgcgtccgct cttcgacagc cgcgtgctgc ggttggagga
     7681 cgaaatcaaa gacgggatct acgagatccg tgccgaggtc cccggtgttg accccgccga
     7741 cgacatcgag gtcaccgtgc ttgatggcca gctgacgatc aaggccgagc ggacccagac
     7801 gggcgagtcc aacgggcact cggaattctc gtacggctcg ttcgtgcgga ccgtcgcact
     7861 gccagcgggt gccgacgagg acgagatcca cgccatctat gacaggggca ttctcacggt
     7921 gtcggtgccg cttgccgagg atcacccgac cgagaagcgt gtcgacgtca tcgagacgat
     7981 cctcgccgac gaggacgacg actttgacga tgacgatgac gatgacgatc acgacgaagc
     8041 cgacgacgac gagcagcccc agctcgagga ggccgaacac actgaggacg aggaagagca
     8101 ccagcccgtc ggctgaaact ggcgcgagcg gcaatgcgct tgcggttttt gcgtgtcctg
     8161 ccatctaccc tcgtagtcgt ggatgcaccg gaactcgtcg ccttgttgca cggccggcgc
     8221 gttgcggtgc tgagcggcgc ggggatgtcc acggattcgg gcatccccga ctaccggggg
     8281 ccggactcgc cgccgagcaa cccgatgacg atccgccagt tcacgtccga cccggcgttt
     8341 cggcagcggt actgggcgcg caaccacctc ggctggcggc acatggacga gacgctgccc
     8401 aatgccgggc accgtgcgct cgccgaactg gagcacgccg gcgtggtgtc cggtgtgatc
     8461 acccagaacg tcgacctgct gcacaccaag gccggtagtc gcaccgtagt gaacctgcat
     8521 ggcacctatg cccaggtgat ctgcctggac tgcgggcaca cgatgtcgcg tgccgcgctt
     8581 gcggatctgc tcgaggcggc caacccgggg tttatcgagc ggacggaggc ggtcggcggc
     8641 atcgcggtcg cacccgacgc cgacgcggtg gtcggcgata cgacgtcctt tcggatcatc
     8701 gactgtcccc attgcggcgg catgctgaag ccgaacatcg tgtacttcgg cgagaacgtg
     8761 cccaaagacc gtgtggatga agcgtattcg cttgtcgaca gagccgacgc actgctggtc
     8821 gccgggtcgt cgctgacggt gtattccgga taccggttcg tccggcacgc cgcgacgctt
     8881 ggtatcccga tcgcgatcat caaccgcggc cacacccgag gtgacgacct cgcgacggtg
     8941 aaggtggacg gcgggtgctc cccgatgctg gcgttgttgg ccgatgaact gcctgcagta
     9001 gcgaattcgg cttaaatggc gaggtgcccg acgaggcctc gtggttgcag tcgctgctgg
     9061 aaacccggcg acccgatccg gggcccgtcg tcggtcggtg ggcgctgggc atcggcgaca
     9121 tggtggccga tcatccgctg acaccagaca aactgcattg gctggcccgc aagctgaacc
     9181 acttcggcgg cgtggcgatc agcgaggagg acgtcgagtt cgacggcgac gacgtcaact
     9241 ggtcggatgt caccgagatc cacacccgca gcctgatcga gtatctgttc accggcggcg
     9301 tggacacgta gatcgacaag ctaccgctgc ggtggttccc gttccgccgc aaggtgatcg
     9361 aggcaatctc gcgggcggcg gtccggcagt gtttcgaggc gaccgcgcag gcgcacggcg
     9421 tcgacgcgat tttggtgcgc atacgttcgc tcagcgggct gttgcaaaat gtggcgcccg
     9481 attttggtga tgtggccgtg tcgtaggtcg gtgtgattg
//
LOCUS       DASRJA010000507         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_664326,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000507 DASRJA010000000
VERSION     DASRJA010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664326"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1769)
                     /locus_tag="VFC01_22230"
     CDS             complement(114..1769)
                     /locus_tag="VFC01_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HZN82372.1"
                     /translation="MWRDHLVEIIITATLFLTVSVMGFVAARWRRPDDLMHLNEWGLG
                     GRSFGGWITWFLVGGDLYTAYTFVAVPALLFGAGATGFFAVPYTIIIYPLVFLVLCRL
                     WSVSHRHGFVTPADFVRARSGSSTMALLVAATGIVATMPYIALQLVGIEAVLKAMHLP
                     GGWPLIVAFAVLAAYTYQSGLRAPALIAFVKDILIYLVILVAIIYIPTKLGGWSAIFG
                     AADAKFDQTPSATDGILLNPANQWQYITLAFGSALALFLYPHSITGVLASRSRDVIKR
                     NMSALPAYSLLLGLIALLGYMAIAAGVKPLPGSTAGSVDNNTVVPVLFDTVFPDWFAG
                     IAFAAIAIGALVPAAIMSIAAANLFTRNIYREYMRPHATPAQEARVSKITSLVVKVGA
                     VLVILLIDPQFSIDLQLIGGVIILQTLPAVALGLYTRWFHKWGLVAGWVVGMGLGTWM
                     LYSIPNAATKRAHFGGSAFPLSKWFDPTSLGMDARTTVYVGFIAVLANLVVAALVTLI
                     CRSTKVADGVDMTAPDDYFADEGDPRITGPSVDELDEGRVPAR"
     gene            complement(1776..2039)
                     /locus_tag="VFC01_22235"
     CDS             complement(1776..2039)
                     /locus_tag="VFC01_22235"
                     /inference="COORDINATES: protein motif:HMM:NF023183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HZN82373.1"
                     /translation="MATREASAPMPPEPPTRTTGSDAAVRSDRSPWNWLLFIPIVLPL
                     IVALFNAREPELFGFPRFYWLQLAFILVGVGTTTLVYQMTKRR"
     gene            complement(2148..>2417)
                     /locus_tag="VFC01_22240"
     CDS             complement(2148..>2417)
                     /locus_tag="VFC01_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005057828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HZN82374.1"
                     /translation="VPFYSSRGTSFSDDCGAGGGFGLSSFLRDGDSVYRTYFTTSRGV
                     DRMRFDFNVLDLTPYGRQEDWEDSPAGWPQTKPYEWWRLHDEYAS"
BASE COUNT          452 a          818 c          800 g          347 t
ORIGIN      
        1 cccattctgt cggcccgaac aggcgcggtc attccggcga cgcggcccgc ccgaacagat
       61 cttggatgat ctggggagct ggggcagcac agatcatcca aggtcgtggc gtgctaccgg
      121 gcggggaccc ggccttcgtc gagctcgtcc accgacggac cggtgatgcg cggatcgccc
      181 tcgtcggcga agtagtcgtc gggtgcggtc atgtcgacgc cgtcggccac cttcgtcgag
      241 cggcagatca gcgtcaccag cgccgcgacg acgaggttgg cgagcaccgc gatgaatcca
      301 acgtagacgg tcgtgcgcgc gtccatcccc agcgacgtcg ggtcaaacca cttcgacagc
      361 gggaaggccg acccgccgaa gtgcgctcgc ttcgtggcgg cgttcgggat cgagtacagc
      421 atccacgtgc cgaggcccat cccgacgacc cagccggcga cgaggcccca cttgtggaac
      481 caccgggtgt agaggcccag cgcgaccgcg ggcagcgtct gcaggatgat gacgccaccg
      541 atcagctgca ggtcgatgga gaactgcgga tcgatgagca ggatcaccag caccgcgccc
      601 accttgacga ccagcgatgt gatcttgctg accctcgcct cctgcgccgg agtcgcgtgt
      661 ggacgcatgt actcgcgata gatgttgcgg gtgaacaggt tcgccgccgc gatcgacatg
      721 atggcggccg gcaccagcgc accgatcgcg atcgcggcga acgcgatccc cgcgaaccag
      781 tccgggaaca ccgtgtcgaa cagcaccggc accaccgtgt tgttgtccac gctgcccgcc
      841 gtcgaaccgg gcagcggctt cactccggcg gcgatcgcca tgtaccccag cagcgcgatc
      901 aggccgagca gcaggctgta ggccggcaac gccgacatgt tccgcttgat gacgtcccgg
      961 ctcctgctcg ccagcacacc cgtgatgctg tgcggataca ggaacagcgc cagagccgac
     1021 ccgaacgcca gcgtgatgta ctgccactgg ttcgccgggt tcagcaggat gccatcggtc
     1081 gcggatggtg tctggtcgaa cttcgcgtcc gccgcgccga agatggcgct ccagccgccg
     1141 agcttcgtcg ggatgtagat gatcgcgacc aggatcacca ggtagatgag gatgtccttc
     1201 acgaacgcga tcagcgccgg cgcccgcagg ccggactggt aggtgtaggc cgcgaggacg
     1261 gcgaacgcca cgatcagcgg ccagcctccg ggcagatgca tggccttcag cacggcctcg
     1321 atgccgacga gctgcagcgc gatgtagggc atcgtggcca cgatcccggt cgccgcgacc
     1381 agcagcgcca ttgtggacga tcccgaccgg gcccgcacga agtcggccgg cgtcacgaac
     1441 ccgtgccggt gcgagaccga ccagagccgg cacagcacca ggaacaccag cgggtagatg
     1501 atgatcgtgt acggcacggc gaagaagccg gtcgcaccgg caccgaacag cagcgccggc
     1561 acggccacga acgtgtaggc cgtgtagagg tcgccgccga ccaggaacca ggtgatccag
     1621 ccgccgaagc tgcgaccgcc gagcccccac tcgttcaggt gcatcagatc gtctgggcga
     1681 cgccaccgcg ccgccacgaa ccccatgacg ctcacggtga ggaacaacgt cgccgtgatg
     1741 atgatctcca cgagatggtc acgccacatg gcccgtcacc gcctcttcgt catctggtag
     1801 acgagcgtcg tcgtcccgac gccgacgagg atgaaggcga gctggagcca gtagaaccgc
     1861 ggaaagccga acagttccgg ctcccgcgcg ttgaagagcg cgacgatcag cggcagcact
     1921 atcgggatga acagcagcca gttccagggg ctgcgatcgg accggacggc cgcatcggat
     1981 ccggtggttc gcgttggcgg ctcgggtggc atgggcgcag aggcctcacg ggtggccatg
     2041 acaggcacct ccggcggaac tgtggcgact ttgcccctag gtacccacca cccgacccgg
     2101 tccaaccatc ggaattcgac cagatcaatg acccgtacgg agtgtgatca gctcgcgtac
     2161 tcgtcgtgca ggcgccacca ctcgtagggc ttggtctgcg gccagccggc cggcgagtcc
     2221 tcccagtcct cctggcgccc gtacggggtc agatccagca cgttgaagtc gaatcgcatc
     2281 cggtcgacgc cgcgtgacgt ggtgaagtac gtgcggtaga cggagtcgcc gtcgcgcagg
     2341 aacgagctca gcccgaaccc gccacccgca ccgcagtcgt cgctgaagct cgtgccccgc
     2401 gacgagtaga acggcac
//
LOCUS       DASRJA010000508         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3311259,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000508 DASRJA010000000
VERSION     DASRJA010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3311259"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VFC01_22245"
     CDS             <1..707
                     /locus_tag="VFC01_22245"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZN82375.1"
                     /translation="IVAASGRLVAHDITTGTPRWSRKTGGGGYSSPHLMPIDSVDQIV
                     LQHGAGAMSVAPADGAVLWQHTWETGVAIVQPVLADHDAVVIVGGDAMGGRGLRRLAI
                     SYGDGGWHAEERWTSTGLKPYFNDFVIHKGHAYGFDNSILACINLEDGARKWKGGRYG
                     HGQMVLLPEQDLLLVLSEEGDLALVKALPDQFTELAKIKAIEGKTWNHPALAGDVLLV
                     RNGEEMAAFRLSLTGQ"
     gene            1047..>2168
                     /locus_tag="VFC01_22250"
     CDS             1047..>2168
                     /locus_tag="VFC01_22250"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZN82376.1"
                     /translation="MTFKLTKILSLIFVCAASVASAQTIDPHVVEFDPSLDHYTTLPT
                     GQPVVDRYDLHFYYSGAAQPFQVMDLGKPTPETDGKIRVDFFAYLLSWPLPGIVYEAR
                     VAAVGPAGTTLSVASNPFSFSGSCAYDMTPNSLSFSASAGTGSVNVWSGPACTWTATS
                     SNSWVTVTSSPGSGNGIVTFSVASNPTASPRAATVAAGNAVATINQAAGTAAVATTVT
                     VAPATTAVAVGSTATLTAAVWDQNGQPMPSAAVTWSVTPTGVVTLTPNGATVTVTGAA
                     AGTATVKATSGTVSGTATVTVQAPVATTVTVAPATTTVAIGSPATLTATVRDQNGQPM
                     PSATVTWSVTPTGVVTLTPSGATVTVTGAAAGTATVKATS"
BASE COUNT          393 a          714 c          709 g          352 t
ORIGIN      
        1 tgatcgtcgc cgcgtcgggc cggctcgttg cccacgacat caccaccggc acgccgcgat
       61 ggtcgcgtaa gaccggcgga ggcggctaca gctcgccgca cttgatgccg atcgatagtg
      121 tcgatcagat cgtcctgcag catggcgccg gcgcgatgag cgtggcgccg gcggatggcg
      181 cggtgctctg gcagcatacg tgggagactg gcgtcgccat cgtgcagccg gtgctggctg
      241 accacgacgc cgtcgtgatc gtcggcggcg acgcgatggg cggacggggt ctgcggcgcc
      301 tcgcgatctc ctacggcgat ggcgggtggc acgccgaaga acgctggacg tcgaccgggc
      361 tgaagccgta cttcaacgac ttcgtcatcc acaagggcca cgcctacgga ttcgacaaca
      421 gcatcctcgc ctgcatcaat ctcgaggacg gcgcacgcaa gtggaaaggc ggccgctacg
      481 gccacggaca aatggtcctg ctgcccgagc aggatctctt gctggtgctc tccgaggaag
      541 gcgacctcgc gctcgtgaaa gcgttgccgg atcagttcac cgagctcgca aagatcaaag
      601 ccatcgaagg caagacgtgg aaccacccgg cactcgccgg cgatgtcctc ctcgtacgca
      661 acggcgagga gatggcggcg ttccgactgt cactcacggg tcagtaacaa ccgcgcgttt
      721 tgcgtggcgc tcgacttggg ccccatgagc gaatcacgag agcccaagct ccaccgcccg
      781 gtctccaaaa actctttcgc tgtccgttaa tgtccgcgat tgtcgagcat cgcctcgctc
      841 ggcggaagcc tcgcgcccca ccaagtcaag aacgttcgat cggcctacga aattttccgc
      901 gaacggcgta accggtaact cgcccggcac ggggaagacc gcgctgtgtg ctctttcctc
      961 aggaaaaccg cggcagaccg gcttggtact ggcgttgccc tgccgctggc tgccccccgc
     1021 aatctctgcg ccctcggcgt cgaatcatga ccttcaagct gaccaagatt ctgagcctca
     1081 tcttcgtctg cgcggcctcg gtcgccagcg ctcagacgat tgacccgcac gtggttgagt
     1141 tcgatccatc gttggatcac tacaccacgc tgccgacggg gcagccggtg gtggaccgct
     1201 acgatctcca cttctactac agcggtgcgg cgcagccgtt tcaggtcatg gacctgggca
     1261 aaccgacgcc ggaaaccgac ggcaagatcc gcgtcgattt cttcgcgtac ctgctgtcct
     1321 ggcctctgcc cgggatcgtt tacgaagccc gcgttgcggc cgtcggtccg gccggaacaa
     1381 ccctcagcgt cgcctcgaat cctttctcgt tcagtgggtc ttgtgcctac gacatgactc
     1441 ctaacagcct gtcgttctcc gcgagtgccg ggaccggcag cgtcaacgtg tggtcgggcc
     1501 cagcttgcac gtggaccgcg accagctcga attcgtgggt caccgttacc agtagccccg
     1561 gaagcggcaa cggaatcgtg accttcagcg tagcgtccaa ccccactgcc tccccacggg
     1621 cggcgaccgt tgcggccggg aacgcagtcg cgaccataaa ccaggccgca ggaacagcgg
     1681 cggtagcgac gacggtgacc gttgcaccgg ccacaacagc ggtggcggta ggaagtacag
     1741 cgacgctgac cgcggcggtc tgggatcaga atgggcagcc gatgccgagc gcggcggtga
     1801 catggtcggt gacgccgacc ggtgtcgtca cgctcacgcc gaatggcgcg accgtcacgg
     1861 tgactggggc ggcggccggc acggcaacgg tcaaggcgac gagcggcacg gtcagcggca
     1921 cagccacggt tacggtgcaa gcacccgtgg cgacgacggt gacggttgca ccggccacaa
     1981 caaccgtggc gattgggagt ccagcgacgc tgacggccac ggtgcgcgat cagaatgggc
     2041 agccgatgcc gagtgcaacg gtcacgtggt cagtgacacc gaccggcgtc gtcacgctga
     2101 cgccgagtgg cgcgacggtc acggtgaccg gggcggcggc cgggacggca acggtcaagg
     2161 cgacgagc
//
LOCUS       DASRJA010000509        12425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_633529,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000509 DASRJA010000000
VERSION     DASRJA010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12425
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633529"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..791)
                     /locus_tag="VFC01_22255"
     CDS             complement(72..791)
                     /locus_tag="VFC01_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN82377.1"
                     /translation="MGETTVLDARGVLDQSTYLALRDKIIQQALEEPRAVVVDVTELH
                     VPAESAWLVFSSARWHVDRWPTIPLGLVCREATRRSAIADSSLAGRVPVYPTVDAAVR
                     ALGSTAASRKRRRARAELPPTLESLRRARELVMGWLTAWSLTDLIPVTKIVVTAFVEN
                     VLQHTDSMPMIRLETDGAAVIVAVADSSHRRAEICEAASMAGSPPSGLHIVSALCRAW
                     GNAPTSSGKTVWAVIGPENRL"
     gene            complement(893..1759)
                     /locus_tag="VFC01_22260"
     CDS             complement(893..1759)
                     /locus_tag="VFC01_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN82378.1"
                     /translation="MRFTFAEAMTDPTYYLPLAKAAEAAGYHSMTIPDSVAYPFESDS
                     KYPYTPDGSREFLDGKAFIEAFVMASALCAVTTTLKFNFFVLKLPIRPPALVAKQVGS
                     LNALFDNRLGLGVGTSPWPEDYELMGVPFAKRGKRMDECIEIIKGLTSGDYFEFHGEF
                     YDIPKTKMTPAPNKPIPILVGGHAEAALQRAARCDGWMHGGGQPDELDRLIKRLNQIR
                     KEQGRTGPFEIHVISLDAFTLDGIKRLEDKGVTDVIVGFRIPYIMGQDTEPLDAKIRH
                     LETFAEKVIAKL"
     gene            1819..2091
                     /locus_tag="VFC01_22265"
     CDS             1819..2091
                     /locus_tag="VFC01_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010844782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HZN82379.1"
                     /translation="MSAVSKALYMPLSIASSVAGGLIAGRLFTEIWQRISPSDQEPPD
                     PKDLDRSGREAITAAALQGLVFGLVRAAVDRASAHGYRAVTHEPPT"
     gene            2110..3330
                     /locus_tag="VFC01_22270"
     CDS             2110..3330
                     /locus_tag="VFC01_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN82380.1"
                     /translation="MRTWLTRNVRVLSAVSFLQDTASELLYPLLPIYLTSVLGAPAAV
                     VGAVEGAAEGAASLTKLAAGPLGDRFARRPLIATGYGMAALGKVMVAAASAWPGVLAG
                     RVVDRLGKGIRGAPRDALLVADIDDAARGRVFGFHRAMDTLGAVVGPLLGLAGYELLD
                     HQIAPLLWVAVVPAVLSVALVLLVRESRRVLPRAERRAVFARVADLPRRYWRVTTALV
                     LFGLVNFPDALLLLRLNEIGFSVVEVILAYVTYNAVYAVASYPAGLLADRIPRPAVFG
                     IGLLFFAIGYTGLGLTTDMATAWVLLGAYGLFTACTDGVGKAWISSLVGSELQASAQG
                     VFQGASGFAVLAAGLWAGFLWGADGRLPLLISGIVGGLFALGLLAVAIHDRVGTVNST
                     SSSAKGGSPTPTTE"
     gene            complement(3255..4019)
                     /locus_tag="VFC01_22275"
     CDS             complement(3255..4019)
                     /locus_tag="VFC01_22275"
                     /inference="COORDINATES: protein motif:HMM:NF027496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4873 domain-containing protein"
                     /protein_id="HZN82381.1"
                     /translation="MSANVFDAIVIGGRSVGVSLTKVMILRPDDIVGSEFDEIRHTWT
                     VTTRAGDSHDASVVISYGPAPSPAGMVPYLGVAVHGLPNRFFISGPDVRGQQQYVAQC
                     LNAMARTESTRIEVRHSTQRTYTVRYRPGSTVDWRRIRRKIKSAFDLSSRVTVEDEVY
                     DGPAAVHIDDDIHDVRVRLTGHLDPIDGRYHWQGTVFDELPDENPPQRVRLAVGERIA
                     EARIAERTPWGTYSVVGVGEPPFALDEVEFTVPTLS"
     gene            complement(4016..4906)
                     /locus_tag="VFC01_22280"
     CDS             complement(4016..4906)
                     /locus_tag="VFC01_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HZN82382.1"
                     /translation="MTAPTREEFSERLLKGSVKKSYAPIVDIDWDAPLDPDKFFLPPK
                     TVSLYDTPLWDGMTREQQIELSRQEFVNTLSAGIWFENMLNQALLRKMIHQDPTARAT
                     HYELTELGDETRHMVMFGMAIERAGAKPVRPRLYQRMIINALPFAFRGSLLWVAALIG
                     EEIFDSLQRQMMDDPELQPMVQRLMRIHVTEEARHIQFARDGLRKRSPNMRRIPKMFV
                     ANINGVGGYFFRYLFTNPVPYRRTGLDGRMARRIARNSPHRHEVQVVGFAPLAAFLEE
                     VGLMGWISRRMWRRSGFLPR"
     gene            5028..5771
                     /gene="cobF"
                     /locus_tag="VFC01_22285"
     CDS             5028..5771
                     /gene="cobF"
                     /locus_tag="VFC01_22285"
                     /EC_number="2.1.1.152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043819 - precorrin-6A synthase
                     (deacetylating) activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A synthase (deacetylating)"
                     /protein_id="HZN82383.1"
                     /translation="MTRRIHVIGIGAGDPDYLTAQAVDALNDTQVFFAMDKGSNKGDL
                     VELRRQICERFIREPGYRFVELPDPPRAKDGDYRQAVADWHAARAQVWADAIRNELEP
                     GGIGAFLAWGDPSLYDSTLRILDTVATHVDIDYDVIPGITAIQALTARHRIPLNDVGE
                     PVLVTTGRQLRDTGLSGSAVVMLDGDCSFLRCPPETRIWWGAYLGTPDELLVSGTIGD
                     VGAGIAELRADARARHGWIMDTYLLRPAD"
     gene            5777..6169
                     /locus_tag="VFC01_22290"
     CDS             5777..6169
                     /locus_tag="VFC01_22290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZN82384.1"
                     /translation="MGSFLLRAALTGVALWVVTLVVPGIDFVGGDTTLARVGIIFVVA
                     VIFGLVNAIIKPIVQIISIPLYILTLGLIHIVINALMLWITSWITEHTTHWGLYIEDF
                     WWTAIWAAIVLSIVSWLLSLIAGDRVRD"
     gene            6235..6615
                     /locus_tag="VFC01_22295"
     CDS             6235..6615
                     /locus_tag="VFC01_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN82385.1"
                     /translation="MACRISELVLGCRDPEVLARFWCEVLDFVVLDREDDGSVEIGPR
                     EGFGGPQPTIILSRRDEPEKGKSRLHIDVNATDRNQDAELERLLKLGARPADIGRTGE
                     EQWHVLTDPEGNAFCLLKARLDPL"
     gene            complement(6646..7818)
                     /locus_tag="VFC01_22300"
     CDS             complement(6646..7818)
                     /locus_tag="VFC01_22300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN82386.1"
                     /translation="MHSPDFYAGDPYPAFKELRATAPVCWNEDTAFWALLKYEDIRFV
                     SANPMIFSSAKGITIPDPALPNPVQEGNLIFTDPPRHRQLRKLINSGFTRRQVALLEP
                     KARNFARRVLDEVDPSTTQEFAEQVAAPLPTWMIAELLGAPPEDWEQFRAWSDATVGT
                     ADPEIEMDSFVALGQLYEYFQELIKARRAGSTKSPDDLLSVLIAAEMDGQRLSDDDLL
                     DFCYLLLVAGNETTRNLIALGTLALIEHPAQFRKLRDDPSLVPTAVEEMLRFTNPVAS
                     MARCATQDVELRGQAIREGEVVVMLYGSANRDEDIFGPTADEFDIARNPNPHIAFGCG
                     EHSCLGAQLARLEARVMFSELLHRFPEIELVGEVSRMKATIVPGVKRMPVRLMIGV"
     gene            complement(7827..8393)
                     /locus_tag="VFC01_22305"
     CDS             complement(7827..8393)
                     /locus_tag="VFC01_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82387.1"
                     /translation="MPPLGYGGLDRDHLTKHLLALAQRVGVDQVTMRDLAAEAGTSAS
                     SVYYHVSGKTEMLDLLIEAVVNTIELPSKGEWDQRIVALYVNGWRAFVAVPGIAGLLQ
                     RRPVAASTAILDRASRHILRESGLPANDVAAAHALLFTHLLGSVELQHSRTKTLGEVD
                     LGGDPESVFVYGLRVILTGIRHMEGARL"
     gene            8473..8751
                     /locus_tag="VFC01_22310"
                     /pseudo
     CDS             8473..8751
                     /locus_tag="VFC01_22310"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
     gene            8905..9372
                     /locus_tag="VFC01_22315"
     CDS             8905..9372
                     /locus_tag="VFC01_22315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HZN82388.1"
                     /translation="MPGIAALGPDALSLSPEELAGVLAGNSGRIKTVITDQKVIAGIG
                     NAYSDEILHVAKLSPFATAGKLTDAQLAALHDAMISVLSDAVERSVGQQAATLKGEKR
                     SGLRVHARTGLPCPVCGDTVREVSFADKSFQYCPTCQTGGKVLADRRLSRLLK"
     gene            9382..10131
                     /locus_tag="VFC01_22320"
     CDS             9382..10131
                     /locus_tag="VFC01_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN82389.1"
                     /translation="MTRQKILITGASSGLGAGMARAFAAKGRDLALCARRTDRLDELK
                     AELLERHPAITVAVAELDVNDHEQVPKVFTALADELGGIDRIIVNAGIGKGAPLGSGK
                     LWANKATIETNLVAALVQIETAIEMFKAAGGGHLVLISSVLANKGVPGVRAAYAASKA
                     GVSSLGESLRAEYAKGPIKVTVIEPGYIESEMTAKADTTSLMVDNETGTKAMVDAIER
                     ERGRAAVPTWPWAPLVQVLRLLPPEFASKFA"
     gene            10182..10559
                     /locus_tag="VFC01_22325"
     CDS             10182..10559
                     /locus_tag="VFC01_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82390.1"
                     /translation="MIVKLTKMTPILFAGAAAAAISFAPAATADDPPPPPPPCLNADG
                     TPCSDIGNVGPGGAEVNIPYGPEGTADAGGASGAIPYGPEGSADGGGASGSLSPYGPG
                     GSADSGGASGCIPNVGCINIPAP"
     gene            complement(10653..>12425)
                     /locus_tag="VFC01_22330"
     CDS             complement(10653..>12425)
                     /locus_tag="VFC01_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN82391.1"
                     /translation="AKGYRPVVRDQPFLFPPDMRDWLPSTHPVWTVLSIVARLDTSAF
                     HQRRRVGGVGRAGYDPDMLLTLLIWAWSQGVRSSRRIERACADVVSYRVICAGDGPDH
                     ATIARFRADNHAACQALFTQVLMLAARLGLGRLETVALDGVKVASNASLAANRTASGL
                     AKAAEAEAARIAEKAAAEHAATDAAEDAAYGPDNPELVPAELADPGVLAERIDEALAH
                     LDAGATADRVDPDDPASPTASHPESTESVEPEPVPGDRAGRIAAAQARIEAEIAAEQA
                     KRQAMVSQYLARIEAGAKITGRVPVEARVALAQRQLDHAIAASQAKHARWAALNAGPA
                     QQRRSPGRRPVPTEQNARVRQAQAKLDTARAAADDARVKAEADAAADARMANITDPQS
                     RMQPLRGGGWVQGYNCQAVTAADGLILATGVGTSPVDCEYYREMVERAVAAAELVTSH
                     RPPGSPDEAAVEPIGIVLADAGYCSRENLTIPGPDRLIATGKARDVHTAASNNPAEGP
                     AQDDIDPIAAMRHRLRTPEGITTYAQRSHIAETPFGHAKYNLNFRRFTGRGLARACSE
                     WAFHGAVHNIGKILGHLAAAPLPA"
BASE COUNT         2039 a         3959 c         4123 g         2304 t
ORIGIN      
        1 tccccgtccg aagtctgttc gtccattcct gtccatccat ctcggccagc gcggccggtc
       61 cgaatactcg gctagaggcg gttctccggg ccaatgaccg cccagaccgt cttgcccgac
      121 gacgtcgggg cgttacccca tgcgcggcac agcgcggaga cgatgtgcag ccccgatggc
      181 ggcgagcccg ccatcgatgc cgcttcgcaa atttcggccc gcctatgact ggagtccgcc
      241 accgccacga tcaccgctgc gccatcggtc tccagtcgga tcatcggcat gctgtcggtg
      301 tgttgaagaa cattctcgac aaacgccgtg accacaattt tggtcaccgg gatcaggtcg
      361 gtcagcgacc acgccgtcag ccagcccatc actaattcgc gcgctcgtcg caggctctcg
      421 agagttggcg gcaattcggc gcgagcgcgt cggcgcttcc ggcttgcggc ggtgcttccc
      481 agcgcgcgga ccgcggcatc gaccgtgggg tagaccggta ccctgccggc gaggctgctg
      541 tccgcgattg cgctgcggcg ggtcgcctca cggcatacca gcccgagggg aatcgtcggc
      601 cagcggtcaa cgtgccaacg cgcgctgctg aagaccagcc atgccgactc tgctggaaca
      661 tggagttcgg tgacatcgac gacgaccgct cggggctctt cgagcgcttg ctgaatgatt
      721 ttgtcccgca gggccagata ggtggactga tccaagacgc cacgagcgtc gagcacagtg
      781 gtttcaccca catattcgac ggtgacattc agagcctgtg gccaacgagt gtttgccatc
      841 gttgtgggtt taccccgctg accggtccgg taatcagttg cgggtgcacc aattacagct
      901 tggcgatgac cttttccgcg aaggtctcca gatgccggat tttggcgtcg agcggctccg
      961 tgtcctgacc catgatgtag gggatccgga agccgacgat gacgtcggtg acgcccttgt
     1021 cctcaaggcg tttgatgccg tcgagggtga acgcgtcgag ggagatgacg tggatctcga
     1081 agggtccggt gcgcccttgt tccttgcgaa tttgattgag ccgcttgatc aatcgatcga
     1141 gttcatccgg ctggccgcca ccatgcatcc agccgtcgca acgggcggcg cgctgcagcg
     1201 ccgcctccgc gtggccgccg acgaggatcg ggatcggttt atttggcgcc ggcgtcattt
     1261 tcgttttcgg gatgtcgtag aactcgccgt ggaactcgaa atagtctccg gaggtcagcc
     1321 ccttgatgat ctcgatgcac tcgtccatcc gcttgccgcg cttcgcaaat gggacaccca
     1381 tgagttcgta atcctcgggc cacgggctgg tgcccacacc gagcccgagc cggttgtcga
     1441 acagcgcatt gagcgacccg acctgcttgg ccaccagcgc cggcggtcgg atcggcaact
     1501 tgaggacgaa gaagttgaac ttcagcgtcg tcgtgaccgc gcacagcgca ctggccatca
     1561 cgaacgcctc gatgaacgcc ttgccgtcca gaaactcgcg gctgccgtca ggggtgtacg
     1621 gatacttcga atcggactcg aacggatagg caacgctgtc agggatcgtc atcgagtgat
     1681 agcccgcggc ctcggctgcc ttcgccagcg gaaggtagta cgtcggatcg gtcattgcct
     1741 cggcgaaagt gaagcgcact cggccatatt agaacgtgtt ctaagtttag ggcttgcggt
     1801 cgcgggaacg ccgtcgccat gagtgcggta tccaaggccc tgtacatgcc cctgtcgatt
     1861 gccagcagcg tggcgggcgg cctgatcgcc ggccgcctct tcaccgagat ctggcagcgg
     1921 atcagcccgt ctgatcagga gccgcccgat ccgaaggatc tggaccgttc gggtcgcgag
     1981 gcgatcaccg ctgccgcgct tcaggggctg gtgttcggtc tggtgcgggc cgcggtggac
     2041 cgggccagcg cacacggtta tcgggccgtc actcatgagc ccccgacctg acgcgccatt
     2101 atggcgtagg tgcgcacctg gctgacccgc aatgtgcggg tgctctcggc tgtctcgttt
     2161 cttcaggaca ccgcgagcga gctgctctac ccgctgctgc ccatttatct gacgtccgtg
     2221 ctgggcgctc cggccgcggt cgtcggtgcc gtggaaggtg cggcggaggg ggcggcgtcg
     2281 ctgaccaagc tggcggcggg accgctgggc gaccgattcg cgcggcggcc cttgatcgcg
     2341 acgggctatg gcatggcggc actcggcaag gtgatggtgg cggccgcgtc ggcctggccg
     2401 ggcgtgctgg ccggccgggt tgtcgaccgg ctgggcaagg gaatccgcgg cgcgccgcgt
     2461 gacgcgctgc tggtggccga catcgacgac gccgcgcgcg gccgggtgtt cgggtttcac
     2521 cgggcgatgg acaccctggg tgctgtcgtc ggtccgctgt tgggtttggc cggctacgaa
     2581 ctgctcgacc accagatcgc accgctgttg tgggttgcgg tcgtacccgc ggtgttgagt
     2641 gtcgcactgg tcctcctggt gcgcgagagc cggcgggtgc tgccacgtgc cgagcggcgc
     2701 gcggtgttcg cgagggtcgc tgacctaccg cgccgctact ggcgggtgac gacggcattg
     2761 gtgctattcg gcctggtgaa ctttccggat gcactgcttt tgttgcggct caacgagatt
     2821 ggcttctccg tcgtcgaagt catcctggcc tacgtcacct ataacgcggt gtacgcggtc
     2881 gcgagttatc ccgccgggct gctggccgac cgcataccac ggcccgccgt gttcggtatc
     2941 ggcttgctgt tcttcgcgat tggctacacc ggactggggc tgaccaccga catggcgacc
     3001 gcatgggtgt tgcttggtgc gtacggattg ttcaccgcgt gcacggacgg tgttggcaaa
     3061 gcgtggattt cgtcgctggt gggctcggag ctgcaggcca gcgcgcaggg ggtgttccag
     3121 ggtgccagcg gattcgcggt gctggctgcg ggtctgtggg ccgggttcct gtggggtgcc
     3181 gacggccgtc ttcccttgtt gatctcgggg atcgtcggtg gactattcgc gctcgggttg
     3241 ctggccgttg cgattcacga cagggtgggc acagtgaact cgacttcgtc gagcgcgaag
     3301 ggcggctcgc cgaccccgac cactgaataa gtgccccacg gggtgcgttc ggcgatgcgg
     3361 gcttcggcaa tgcgttcgcc gactgccagc ctgacccgct gtggtggatt ttcgtcgggc
     3421 agttcgtcga aaactgttcc ctgccagtga tagcgcccgt cgatcgggtc gaggtggccc
     3481 gtcaatcgaa cccggacatc gtgtatgtcg tcgtcgatat gcaccgcggc ggggccgtcg
     3541 tacacctcgt cctcgacagt cacgcgggag ctgagatcga atgccgattt gatcttcctg
     3601 cggatccgtc gccagtccac tgtgctgccg ggccggtagc gcacggtgta ggtgcgttgg
     3661 gtgctgtgcc ggacctcgat ccgggtactt tcggtgcggg ccatggcgtt caggcattgc
     3721 gcgacgtact gctgctgtcc gcggacatcc ggcccgctga tgaagaagcg gtttggcagt
     3781 ccgtgcaccg cgacacccag gtagggcacc atgcccgcgg gtgatggcgc cggaccataa
     3841 gagatcacga cgctcgcgtc gtgactgtcg cccgcgcgcg tggtaacggt ccaagtgtga
     3901 cggatctcgt cgaactcaga cccgacgatg tcgtcaggcc gcagaatcat caccttggtc
     3961 agcgagacgc cgacggagcg accaccgatc acgatggcgt cgaacacgtt tgcgctcatc
     4021 ggggcagaaa tccgctgcgt ctccacatgc gccgcgagat ccaacccatc agcccgacct
     4081 cttcgaggaa tgccgccagt ggggcgaagc cgaccacctg aacttcgtga cggtgcggac
     4141 tgttgcgggc gatacgccgc gccatgcggc cgtccagccc ggtacggcga tacggcacgg
     4201 gattcgtgaa cagataccgg aagaagtacc cgcccactcc gttgatgttc gcgacgaaca
     4261 ttttcgggat ccgccgcatg ttcggagagc gcttccgcag accgtcgcga gcgaactgga
     4321 tgtgacgggc ttcttcggtg acgtgaatac gcatgagccg ctgcaccatt ggctgtagct
     4381 cggggtcgtc catcatctgg cgctgaagcg agtcgaagat ctcctcgccg atcagtgcgg
     4441 ctacccacag tagcgatccc ctgaaggcaa acggcaacgc attgatgatc atccgctgat
     4501 acagcctggg ccgcaccggc tttgcgccgg ctcgctcgat cgccatgccg aacatcacca
     4561 tgtgccgggt ctcgtcgccg agctcggtca gttcatagtg cgtcgcacgt gccgtgggat
     4621 cttggtgaat catcttgcgc agcaacgctt gattgagcat gttctcaaac cagatccccg
     4681 ccgacagggt gttgacgaac tcctgtcgcg acaactcgat ctgttgctcg cgggtcatgc
     4741 cgtcccataa cggcgtgtcg tacagcgaga ccgtcttggg cggcaggaag aacttgtccg
     4801 ggtccagcgg agcgtcccag tcgatgtcga cgatcggggc ataggacttc ttaaccgagc
     4861 ccttcagcaa ccgctccgag aattcctcac gagtgggtgc ggtcatcagc agcctctccc
     4921 gtcgtccttc gttgacgttg ttcaaaaggt agatgcggcg ccgccgatag tcaatacccc
     4981 gggtaccgca tactttgcgt aaccgcaggt catggcatac tcgccgcgtg actcggcgga
     5041 tccatgtcat cggcatcggc gcaggcgatc cggactatct aacggcacag gctgttgatg
     5101 cgctgaacga cacgcaggtg ttctttgcga tggacaaggg ttcaaacaag ggcgaccttg
     5161 tcgaacttag gcggcagatc tgcgaacggt tcatccgcga gcctggctac cggttcgtcg
     5221 aactgcccga cccgccgcgc gccaaggacg gcgactaccg acaggcggtc gccgattggc
     5281 acgcggcgcg ggcacaggta tgggccgacg ccatccgaaa tgagctggag cccggcggga
     5341 ttggcgcgtt cttggcgtgg ggcgatccgt cgctctacga cagcacgttg cgaatcctgg
     5401 acaccgtcgc gacgcatgtc gacatcgact acgacgtcat ccccggcatc accgcgatcc
     5461 aggcgctgac ggcgcgacac cgcatcccgc tcaacgacgt cggcgaaccg gtgctcgtca
     5521 ccaccggtag gcaactacgc gacaccgggc tatccggcag cgccgtggtg atgctcgacg
     5581 gtgactgttc gttcctgcgc tgcccacccg aaactcgcat ctggtggggc gcttacttgg
     5641 gcacgcccga cgagctgctg gtgtccggca caatcgggga cgtgggcgcc gggatcgcgg
     5701 aactgcgggc cgacgcgcgg gcgcgacacg gctggatcat ggacacctac ctgctgcgcc
     5761 cggcagacta gcggccatgg ggagcttcct gctgcgcgca gcgcttaccg gggtcgcgct
     5821 gtgggtggtc accctcgtcg ttcccggcat cgacttcgtc ggcggggaca ccacattggc
     5881 gagggtcggc atcatcttcg tcgtcgcggt gatcttcggt ctggtcaacg cgatcatcaa
     5941 gccgatcgtg cagatcattt cgatcccgct gtacatcctc acgctcggtc tgatccacat
     6001 cgtcatcaat gcgttgatgt tgtggatcac ctcgtggatc accgagcaca ccacccactg
     6061 gggcctgtac atcgaagact tctggtggac cgcgatctgg gcggcgatcg tgctgtcgat
     6121 cgtgagctgg ctgctgtcgt tgatcgccgg cgaccgcgtc cgcgactagg tcgtgtctca
     6181 agaggttcgc gtgaggcctc cccggcagtt cccaggctga gaggaggttt cgttatggca
     6241 tgtcgcatca gtgagctcgt gctcggttgc cgcgaccctg aggtgctggc gcggttctgg
     6301 tgcgaggtcc tggacttcgt cgtgctcgat cgcgaggacg acggctcggt ggagatcggg
     6361 ccgcgcgaag ggttcggtgg tccgcagccg acgatcatcc tcagtcgcag ggatgagccg
     6421 gagaagggga aatcccggct gcacatcgac gtcaacgcca ccgaccgcaa tcaggacgcc
     6481 gagctcgaac gccttctgaa gcttggtgcg cgcccggctg acatcggccg gacaggggag
     6541 gagcagtggc atgtcctgac cgaccccgaa ggcaatgcgt tctgcctgct taaggcccgc
     6601 ctcgacccac tctgatgccc tcggtcacgc ctcgccacca ggtacctaga cgccgatcat
     6661 cagccgcacg ggcattcgtt tgacacccgg cacgatggtg gctttcatcc gcgacacttc
     6721 gcccacgagt tcgatctctg ggaaccggtg cagcaactcc gaaaacatca cccgggcttc
     6781 cagtcgcgcc agttgcgcgc ccaggcatga gtgttctccg cacccgaagg cgatgtgcgg
     6841 gttggggttt cgcgcgatgt cgaactcgtc cgcggtagga ccgaagatgt cctcgtcccg
     6901 gttggccgat ccgtacagca tcaccaccac ctcgccctcg cggatggcct ggccgcgaag
     6961 ttcgacgtcc tgggtggcgc agcgggccat gctggcgacg gggttggtga acctgagcat
     7021 ttcctcgact gcggtgggga ccaggctggg atcgtcacgc aacttccgaa actgtgcggg
     7081 atgttcgatg agggccagcg tccccagcgc gatcaggttt cgggtggtct cgttgcccgc
     7141 caccaacagc agatagcaga agtcgagcaa gtcgtcgtcg ctgagccgct ggccgtccat
     7201 ttccgctgcg atcaggaccg acaacagatc gtccgggctc tttgtcgaac cggctcggcg
     7261 ggccttgatc aattcctgga aatactcgta gagctgcccc agcgcgacga acgaatccat
     7321 ttcgatctcc ggatcggcgg ttccgaccgt cgcgtccgac cacgcacgga actgctccca
     7381 atcctcgggc ggcgcaccca ggagctccgc gatcatccag gtgggcagtg gcgcggcgac
     7441 ctgttcggcg aactcttgcg tggtcgacgg atcaacctcg tcgagcacgc gtcgcgcgaa
     7501 gttgcgtgcc tttggctcga gcagtgcgac ctgccgccgg gtgaaacccg agttgatcag
     7561 cttgcgcagc tgccggtggc gtggcgggtc ggtgaagatg agattcccct cctgcaccgg
     7621 gtttggcagc gcgggatcgg ggatcgtgat gcccttggcc gacgagaaga tcatcgggtt
     7681 ggccgacacg aacctgatgt cttcgtactt caacagcgcc cagaacgcgg tgtcctcgtt
     7741 ccagcacacc ggcgccgtcg cacgaagttc tttgaacgcg gggtagggat cgccggcgta
     7801 gaagtctggg gagtgcagcg ggaccgtcac agtcgtgctc cttccatgtg acgaatgccg
     7861 gtgaggatta cccgcaggcc atagacgaag acggattccg ggtcgccacc cagatccacc
     7921 tcgccgagtg tcttggtgcg actgtgttgc agctcgaccg aacccagcag atgggtgaac
     7981 agcaacgcgt gtgcggccgc gacatcgttt gcgggcaagc ctgattcgcg caatatgtgg
     8041 cgcgacgccc ggtccaggat ggctgtggac gccgcgaccg gccgtcgctg cagcagacct
     8101 gcaattcctg gaacggcgac gaaggcacgc cagccgttga cgtagagcgc gacgatgcgc
     8161 tgatcccact cgcctttcga tgggagttcg atggtgttca ccacggcttc gatcagcagg
     8221 tcgagcatct ccgtttttcc gctgacgtgg tagtacaccg acgacgcgga cgtgcctgct
     8281 tcggcggcga ggtcgcgcat cgtcacctgg tcgaccccga ctcgttgcgc cagggccagc
     8341 aggtgcttgg tgaggtgatc gcggtccaac ccgccgtacc cgagcggcgg catggtgcgc
     8401 tccgatctgc ccactatcgc ctttcgaacg ttgttccaat tcgaacgctg ttcagattcg
     8461 cacagtgagc gcatggacgt caacggtggc gatcggcaga ctggacgagt gcccgaacta
     8521 cctgagatcg aagcgctggc cgatcatctg cgccgtcacg ccgtcgggtg cagcatcggg
     8581 cgcatcgacg tatcggcgct gtcggtgctc aagacgttcg atccgccgat caacgtgctg
     8641 cacggtcaga ccgtcaccgg cgccgggcgc tggggcaagt acctcggcat gcaagccggc
     8701 gacctgcacc tcatcacaca cctgtcgcgg gcaggttggc tgcgatggtc ggacaagctg
     8761 gccgcggcgc cgctgaagcc gggcaagggt ccgatcgcgt tacgcgtcca cctcggaaag
     8821 ccaggcgatg caccgggttt cgacctgacc gaggcaggaa cccaaaagcg gttggcggtg
     8881 tggttggtcg acgatccggc caaagtgccg ggtatcgcgg cgctgggtcc agacgcgttg
     8941 tcgttgagtc cggaggaatt ggcgggcgtt ctggcgggca acagcgggcg gatcaagacc
     9001 gtgatcaccg accagaaggt gatcgcagga ataggcaacg cctacagcga cgagatcctg
     9061 catgtcgcca aactgtcgcc gttcgccacc gcgggcaagc tgaccgacgc acagctggcg
     9121 gcgctgcacg acgcgatgat ctcggtgctg agtgatgctg tcgaacgctc cgtcggccag
     9181 caggcggcga cgctgaaggg ggagaagcga tcagggctgc gcgtgcacgc ccgcaccggt
     9241 ctgccgtgcc cggtctgcgg cgacaccgtg cgtgaagtgt cgttcgccga caagtcgttc
     9301 cagtactgcc cgacctgtca gaccggcggc aaggtactgg ccgaccggcg gctgtcccgg
     9361 ctgctcaaat aggctgtgcc gatgactcgg cagaaaatcc tgatcaccgg tgcctcgtct
     9421 gggctgggcg caggcatggc ccgcgctttt gctgcgaagg ggcgtgatct ggcgctgtgc
     9481 gcccgccgca ccgatcgtct cgacgagctg aaggctgaac tgctggagcg tcatccggcc
     9541 atcaccgtcg cggtcgccga gctggacgtc aacgaccacg agcaggtgcc gaaggtcttc
     9601 acggcgctcg ccgacgaact cggcggcatc gaccgcatca tcgtcaacgc gggcatcggt
     9661 aaaggcgcgc cgctcggctc gggcaagctg tgggccaaca aagccaccat cgagacgaat
     9721 cttgttgcgg cgcttgtaca aatcgagacc gcgatcgaga tgttcaaggc cgccggcggt
     9781 gggcatctgg tgctcatctc gtcggtgctc gccaacaagg gcgtgcccgg cgtgcgcgct
     9841 gcctatgccg ccagcaaggc tggagtgtcc tcgctgggcg aatcgctgcg cgccgaatac
     9901 gccaagggcc caatcaaggt gacggttatc gagccgggct acatcgaatc cgagatgacc
     9961 gccaaggcgg acacgacctc cttgatggtc gacaacgaga ccggcaccaa ggcgatggtc
    10021 gacgccatcg aacgcgaacg tggccgtgcc gccgtgccga cgtggccctg ggcgccgttg
    10081 gtgcaggtgt tacgcctgct gccaccggaa tttgcatcca aattcgccta gttaggcgca
    10141 ctacgggttt aggcacccga cgatcgtggt agccgatcta catgatcgta aaactgacga
    10201 aaatgacgcc catactgttc gcgggcgctg cggcagctgc gatttcgttc gccccggccg
    10261 cgacggctga cgacccaccg cctcctccgc cgccatgcct caacgctgac ggcacgccgt
    10321 gctcggacat cggcaacgtc ggcccgggtg gcgccgaggt gaacattcct tacgggcccg
    10381 aaggcacagc cgatgccggg ggcgcctctg gcgcgattcc ctatggcccg gaaggctcag
    10441 cagatggcgg tggtgcgagc ggatcgctgt cgccgtacgg ccctggcggc tcggctgact
    10501 ccggtggtgc cagcggatgc atcccgaacg tgggctgcat aaacatccca gccccgtaat
    10561 gtgacttcgg cgcgctaaca gccgctgacg ggttgtctcg aaatgattcg tggtgatttc
    10621 gacgggttag gtgctgatgg tgcctgcggt gcttaggcgg gcagtggtgc tgcggcgagg
    10681 tggccgagga tcttgccgat gttgtgcacg gcgccgtgga aggcccattc gctgcatgcg
    10741 cgggctaggc cgcggccggt gaatcgtcgg aagttcaggt tgtatttggc gtgcccgaac
    10801 ggggtttcag cgatgtggga tcgctgggcg taggtggtga ttccctctgg ggtgcgcagc
    10861 cgatgccgca ttgccgcgat cgggtcgatg tcatcttgcg ctggcccctc ggcgggattg
    10921 ttgctggccg cggtatgcac atcgcgggct ttaccggtgg cgatcaaccg gtccggacca
    10981 gggatggtca ggttctcgcg cgagcagtag ccggcatcgg caagcacgat cccgatcggc
    11041 tcgaccgcgg cctcatccgg ggagccgggt ggccggtggc tggtgactaa ttccgctgcg
    11101 gccacggccc tttcgaccat ctcgcggtag tactcacagt caactgggct ggtgcccacg
    11161 ccggtggcca ggattagccc atcggcggcg gtgaccgcct gacagttgta gccctgcacc
    11221 cagcccccac cgcgcagcgg ctgcatccgc gactgcggat cggtgatatt ggccatgcgt
    11281 gcgtcggccg cagcgtcggc ctcggctttg acccgggcgt catcggcggc cgcgcgcgcc
    11341 gtgtcaagct tggcctgggc ctggcgcacc cgggcgttct gctcggtcgg taccgggcgc
    11401 cgccccggac tgcgccgctg ctgggcgggt ccggcattga gcgccgccca gcgggcgtgt
    11461 ttggcctgtg aggcggcgat cgcatggtcc aactggcgct gagccaacgc cacccgcgct
    11521 tcgaccggaa cccgaccggt gatcttcgcc ccggcctcga tacgcgccag atactggctc
    11581 accatggcct gccgcttagc ttgctcggcg gcgatctcgg cctcgatgcg ggcctgcgcg
    11641 gccgcgatgc gccccgctcg atcacccgga accggctcgg gttcaaccga ttccgtggac
    11701 tctgggtgcg acgccgtcgg cgaagcgggg tcatctggat caacccgatc ggcggtagcc
    11761 ccggcatcca ggtgcgcaag tgcttcgtcg atgcgctcgg ccagcacgcc cggatcggcc
    11821 agctcggccg gcaccaattc ggggttgtcg ggcccgtagg ccgcgtcctc ggcggcatcg
    11881 gtggccgcat gctcggccgc ggctttctca gcgatgcggg cggcttcggc ctccgcggct
    11941 ttggccaacc ccgatgcggt gcggttggcc gccaacgagg cattcgaggc gaccttgacc
    12001 ccgtcgagcg ccaccgtctc caaccgcccc aaccccaacc gcgcggccag catcaacacc
    12061 tgggtgaaca acgcctggca ggcggcgtga ttgtcggcgc gaaaccgcgc gattgtggcg
    12121 tgatcaggcc catcaccggc gcagatcacc cgatacgaca ccacatcggc acacgcgcgc
    12181 tcgatgcgcc gcgaggaccg caccccctgc gaccacgccc agatcaacaa cgtcaacaac
    12241 atgtccgggt cataaccggc ccggcccaca ccaccaaccc ggcgacgctg gtgaaacgcc
    12301 gaggtgtcca ggcgcgccac gatcgacagc acggtccaca ccggatgagt cgatggcagc
    12361 cagtcccgca tatccggcgg gaacaagaac ggctgatcac gcacgacagg tcgatacccc
    12421 ttggc
//
LOCUS       DASRJA010000510         3563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3659247,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000510 DASRJA010000000
VERSION     DASRJA010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3659247"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2428)
                     /locus_tag="VFC01_22335"
     CDS             complement(<1..2428)
                     /locus_tag="VFC01_22335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HZN82392.1"
                     /translation="MTADLSLAGRPDYKKKRGELRIYLGAAPGVGKTFAMLGEAHRRL
                     ERGTDVVAAVVETHGRKKTADLIEGIETVPPKYIEYRGKRFPELDVEAVLRRQPQVVL
                     VDELAHTNTPGSKNPKRWQDVEELLDAGITVISTVNVQHLESLNDVVEQITGIEQKEK
                     IPDEIVRAADQIELVDITPEALRRRLAHGNVYAPDRIDAALSNYFRRGNLTALRELAL
                     LWLADQVDAALEKYREENKITDTWEARERVVVAVTGGPESETLVRRASRIASKSSAEL
                     MVVHVVRGDGLAGVSAPQMGKVRELAISLGATLHTVVGDDVPSALLDFARDENATQLV
                     IGTSRRSRWARIFDEGIGASVVQQSGKIDVHMVTHEEAKRGFSWSRLSPRQQHMTSWL
                     AALVVPSAICALIVAFLDPYLKVGGESALFFVGVLVVALLGGVAPAALSAVLSGLLLN
                     YFLIEPRYTLTIAEPDNAITSVVLLVVAVAVAALVDNAAKRTREARHASQEAELLALF
                     SGSVLRGADLDTLLERVRETYSQRAVSLLREGTGIVGCVGKDPCVDVDTADTAIEVGD
                     DEFWLLMAGRKLPARDRRVLSAVAKQAAGLVKQRELTEEAGKAEAIAQADELRRSLLS
                     AVSHDLRTPLAAAKAAVSSLRSSDVDFSAEDTAELLATIEESIDQLTALVGNLLDSSR
                     LAAGVLRPELRRVYLEETVQRALLGISKGITGFRRQGVDRVKVDVDDAVVMADSGLLE
                     RVLANLIDNALRYAPDSPIRVTAGQVGERVLIAVVDEGPGIPRGSEEQLFAPFQRLGD
                     HDTSIGVGLGL"
     gene            complement(2452..3360)
                     /locus_tag="VFC01_22340"
     CDS             complement(2452..3360)
                     /locus_tag="VFC01_22340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HZN82393.1"
                     /translation="MKLSNFARQHWAALRALMVLTVIVGIAYPVFIWLVAQIPGLRDN
                     ANGSIIEANGKPVGSSLIGQSFTDKDGNALPQYFQSRPSAAGDGYDPLATSASNLGPE
                     SIVDTPADPSAPKDDNGYKASLLTLVCGRSKTVGDLEGVNGARPFCTGEGVGAVLSVI
                     GPRDASGNVVHPTKVVSVNEPCETTPTPFLNTYEGVRVECAKFGDDYTIGQIVPIRGS
                     APADSQVPADAVTASGSGLDPHISIAYADLQVNRVAKARGISPDQIKQAVADNTDGRW
                     LGFLGESRVNVLKLNLELDQKYPAKS"
     gene            complement(3362..>3563)
                     /locus_tag="VFC01_22345"
     CDS             complement(3362..>3563)
                     /locus_tag="VFC01_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82394.1"
                     /translation="ILSAVIFNAVVIVALIPLSLRGVRYTPSSASKLLSRNLYVYGLG
                     GIIAPFIGIKLIDLVIQFLPGM"
BASE COUNT          607 a         1160 c         1147 g          649 t
ORIGIN      
        1 acaggccaag cccgacgccg atgctggtgt cgtggtcgcc gagccgctgg aacggtgcga
       61 acaactgctc ctcactaccg cgcgggatgc ctggtccctc gtcgacgacg gcgatcagca
      121 cgcgctcacc gacctgtccg gccgtgacgc ggatcgggct gtccggcgcg tagcgcagcg
      181 cgttgtcgat gaggttggcc aacacgcgct ccagcaggcc gctatcggcc atcacgacgg
      241 cgtcatccac gtcgaccttc actcgatcta ccccttgacg ccgaaaaccg gtgatgccct
      301 tgctgattcc cagcagggcg cgctgcacgg tttcctccag atagacgcgt cgcagctcgg
      361 gccgaagcac accggcagcg agccgcgacg aatccagcag attgccgaca agcgccgtca
      421 gctggtcgat ggattcctcg attgtggcga gaagttcagc ggtgtcttcg gcggagaagt
      481 cgacgtcgct gctgcgcagg ctggacaccg cggccttggc cgcagccagg ggagtgcgca
      541 ggtcgtggct gaccgccgac agcagcgaac ggcgcagttc gtcggcctgc gcgatcgcct
      601 cggccttgcc ggcctcttcc gtcagctcgc gctgcttgac caggcccgcc gcttgcttag
      661 cgaccgcact cagcacgcgg cggtcgcggg cgggcaactt gcgcccggcc atcagcagcc
      721 agaactcgtc atcgccgacc tcgatcgcgg tgtcggcggt gtcgacgtcg acgcagggat
      781 ccttgcccac acacccgacg atgccggtac cttcccgcag caggctgacg gcgcgctgtg
      841 aataggtttc gcgcactcgc tccaacagcg tgtccaggtc ggcaccgcga agcaccgacc
      901 cggagaacaa cgcgagaagt tcggcctcct gcgatgcgtg cctggcttcg cgggtgcgct
      961 tcgcggcatt gtccacaagt gctgcgacgg cgacggcgac gaccagcagc accacgctcg
     1021 tgatcgcgtt gtcgggctcg gcgatggtga gcgtgtagcg cggttcgatc aagaagtaat
     1081 tcagcaacag cccggacagc acagcggaca gcgcggcggg tgcgacaccg cctagcaacg
     1141 ccaccacgag cacgccgaca aagaagagag cgctctcgcc gccgactttg aggtaagggt
     1201 ccaggaatgc gacgatcagc gcgcagatgg cggacggcac cacaagtgcg gccagccacg
     1261 acgtcatatg ctgctggcgc ggcgagagcc gtgaccacga gaagccgcgc ttggcttcct
     1321 cgtgggtgac catgtgcacg tcgatctttc ccgactgctg taccaccgag gcgccgatgc
     1381 cctcgtcgaa gatgcgggcc catcgcgacc gccgcgacgt gccgatcacg agctgggtgg
     1441 cgttctcatc gcgggcgaaa tccagtaggg cgcttggcac gtcgtcgccg acgacggtgt
     1501 gcagggtggc accgaggctg atagccagtt cccgtacctt gcccatctgc ggtgcggaga
     1561 ccccggccag cccgtcgccg cgaacgacgt ggacgaccat cagctccgcg ctggacttag
     1621 acgcgattcg agatgcgcgg cgtaccaagg tctccgactc cggtccgccc gtgacggcga
     1681 cgacgacacg ctcgcgtgcc tcccaggtat cggtgatctt gttctcttcg cggtacttct
     1741 ccagggcggc gtcgacctgg tcggccagcc agagcagcgc caattcacgt agcgccgtga
     1801 ggtttccgcg ccgaaagtag ttcgacaacg ccgcgtcgat gcggtcgggg gcatagacgt
     1861 ttccgtgggc gagcctgcgc cgcaacgctt ctggtgtgat gtcgacgagt tcgatctggt
     1921 cggctgcgcg gacgatctcg tcgggaatct tctccttctg ctcgatgccg gtgatctgtt
     1981 ctacgacgtc gttgaggctt tccagatgct gcacgttgac cgtcgagatg accgtgatgc
     2041 cggcgtcgag tagctcctcg acgtcctgcc accgcttggg gttcttgctc ccgggagtgt
     2101 tggtgtgcgc gagctcgtcg acgagcacga cttgcggctg ccgccgcagc acggcctcga
     2161 catcgagttc ggggaagcgc ttaccgcgat attcgatgta cttgggcggc accgtctcga
     2221 tgccctcgat caagtccgca gtcttcttgc ggccatgcgt ctcgacgacc gccgcaacta
     2281 catcggtgcc gcgttccagc cgccgatgtg cctcgccgag catcgcgaat gtcttcccga
     2341 cacccggcgc ggcacccaag tagatgcgca gctcaccgcg cttcttcttg tagtcgggcc
     2401 tgccggccag cgacaggtcg gctgtcacat gctcatcatc caccggacac gtcagctctt
     2461 ggcgggatac ttctggtcca gttccaggtt gagcttcaac acgttgaccc tggactcacc
     2521 taagaacccg agccagcgtc cgtcggtgtt gtccgcgacc gcctgcttga tttggtcggg
     2581 gctgattccg cgcgccttgg cgacccggtt cacctgcaga tcggcgtacg cgatcgagat
     2641 gtgtgggtcg aggccgctac cgctggcggt gacggcatcg gccggcacct gagagtcggc
     2701 gggggcgctt ccgcggatcg gcacaatctg gccgatggtg taatcatcac cgaactttgc
     2761 gcactcgacg cggacgccct cataggtgtt gaggaatggc gtcggagtcg tttcgcacgg
     2821 ttcgttgaca ctgaccacct tggtggggtg aaccacattg cccgacgcgt cgcgcgggcc
     2881 tatcaccgac agcacggccc cgactccctc accggtgcag aacggacgcg caccgttcac
     2941 accctcgagg tcgccgaccg tcttgcttcg cccgcatacc agggtgagca ggctggcctt
     3001 gtagccgttg tcatccttcg gcgcggacgg atcagccggg gtgtcgacga tgctctccgg
     3061 cccgagattg ctcgcggagg ttgccagtgg atcgtagccg tcacccgcgg ccgacggccg
     3121 gctctggaaa tactgtggca gagcgttgcc gtccttgtcg gtgaacgatt gaccaatgag
     3181 gctgctgccc accggctttc cgttcgcctc gatgatcgac ccgttggcgt tgtcccgcag
     3241 acccggaatc tgggcgacca gccagatgaa gacggggtag gcgatgccga caataacggt
     3301 gagtaccatc agcgcccgca atgcggccca gtgttgacga gcgaagttcg atagtttcat
     3361 atcacatccc tggtaagaat tggattacga ggtcgatgag tttgatgccg atgaacggcg
     3421 cgatgatgcc gccgagaccg tagacgtaga ggttgcgact caacagcttt gatgcactgc
     3481 tgggtgtgta acgaacaccg cgcagcgaga gcgggatcag cgcgacgatg accaccgcgt
     3541 tgaagatcac cgccgacagg atc
//
LOCUS       DASRJA010000511         5054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1165562,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000511 DASRJA010000000
VERSION     DASRJA010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165562"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFC01_22350"
     CDS             complement(<1..486)
                     /locus_tag="VFC01_22350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZN82395.1"
                     /translation="MPNSFDLDGRGPSNHQLFVAGLCFLLVGAIVVTLLIAKSKGRLD
                     HIVRVSAELADVGDGLPAKSDVKFRGVLVGHVAGVIPAGPGQYNIAHLDLSGEYASAI
                     PNTVTARVVPSNVFAVSSVQLVDNGNGSAPLRDGAAIPEDQSLPTVLFQTTLTKFRDV
                     LA"
     gene            complement(489..1343)
                     /locus_tag="VFC01_22355"
     CDS             complement(489..1343)
                     /locus_tag="VFC01_22355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN82396.1"
                     /translation="MTVASYRVWGTRAFVDAADAVMRTVAKAGHIMYFVGRVVTSIPL
                     MLRSYPREFFRILSDIAWGNGSIVVGGGTVGVALVLGVTAGALVGVEGYNALNLLGLG
                     PATGVLASWGSTRELAPVMLALAFTAQAGCRFTAQLGAMRIAEEIDALDTLGINPIRY
                     LVTTRVAASVVAAIPLFLACLAVTYLSAQIVVAFSGQTSGTYQHYFAMFASGRDVAYA
                     TFKAGVFVFVAATIQCYFGYFASGGPQGVGVAAGRAMRASITVVIILNLLLTMAMWGV
                     NAGARFGG"
     gene            complement(1346..2263)
                     /locus_tag="VFC01_22360"
     CDS             complement(1346..2263)
                     /locus_tag="VFC01_22360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN82397.1"
                     /translation="MALRSADRQDAGSLTADESTVIASHPPVEAPSAREEIRGWGRHY
                     IRAHPLLSLHTMGGQVVLGVRTAQYFFTDLATGRFMVGEFVQQAAFMAGTAFVPTLLV
                     TLPIAATLSIQFSLLAGQIGATSLAGAATGLFVLRQGAPLVAAILLAAAVGSAVSADL
                     GSRTMREEIDAMEVMGVSVIRRLVVPRFAAVIVIGIALTGLTCFVGFIAGYVFNVYMQ
                     NGTPGSFVATFAAFATPQDLVVALIKAMVFGAIVAIVSCYKGLETRGGPAGVANSVNA
                     AVVEAILLLMLVNVAISKLYILIFPRATL"
     gene            2782..3510
                     /locus_tag="VFC01_22365"
                     /pseudo
     CDS             2782..3510
                     /locus_tag="VFC01_22365"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(3542..>5054)
                     /locus_tag="VFC01_22370"
     CDS             complement(3542..>5054)
                     /locus_tag="VFC01_22370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZN82398.1"
                     /translation="DWSYELCPYNAQVLWRRLSVFPGTFDLELAESVCAFGELDPDEV
                     IDSIERLVGQSILLTGQAAGAMRYRLLAPLREVAGELAEQAGETAELQRRHHNAMLRR
                     AQQVVQQWCGPQQDVLIARMGLDHANYVAALQWSAATEGEEQTGLLLLAALRYHWLSG
                     GLLAEGRMRIETMLAAVGEPSAARAECLWVASWIALVQGDRHQTERWLEELAVLAKQS
                     DDPRLDLHVYHWTGLLAMFTGDVDGAVRGLRVAVDGYRARGDREMELTARYALATALM
                     VGGHAEDALTISSETVALCDENGERNARAYAQWAAGVAHWTLGDLDETERSARKVLKT
                     HRTMADGIGVALTTELLSWIAYDRKQVAHAEALSAAARHVWRTLGTSIEAFGPQLSRF
                     AEGHAPPRRAARVPDEGHVAHRFRDLDDVIDFVLGVGGDRVGPGRTDDAALLTKRELE
                     VAELIESGLSNREIAERLVISKRTAEGHVERILAKLGFSSRAQVAVWMARRAS"
BASE COUNT          980 a         1638 c         1556 g          880 t
ORIGIN      
        1 cgccaacacg tcacggaatt tcgtcaaggt ggtctgaaag agcaccgtcg ggaggctttg
       61 atcctcgggg attgcggccc cgtcccgcag tggcgccgag ccgttcccgt tgtccacaag
      121 ttggaccgac gacaccgcga atacgttgga aggcaccacc cgcgcagtca cggtattggg
      181 gatggcagag gcgtactcac cgctgaggtc aaggtgagcg atgttgtatt ggccggggcc
      241 cgctgggatg accccggcaa catggccgac cagtacgccg cggaacttga cgtccgactt
      301 ggccggtagg ccgtcgccaa catcggctag ctcagcgctg accctcacga tatggtccag
      361 tctgcctttg gatttcgcga tcaacagagt cacaacgatt gcaccaacca gcagaaaaca
      421 cagtcctgca acaaagagct gatggttaga cggcccgcgg ccatccaggt cgaaggagtt
      481 cggcattgct acccgccaaa cctcgcgcct gcgttgaccc cccacatcgc cattgtgaga
      541 agcagattca agatgatgac aacagtgata ctcgcacgca tcgcccgtcc ggccgccaca
      601 cccactccct gaggaccgcc ggaggcgaag tagccgaaat agcactgaat tgttgccgcg
      661 acgaacacga agacaccggc tttgaaagtc gcgtacgcca catccctacc gctggcaaac
      721 atcgcgaaat agtgttggta tgtgccggaa gtttgtccgg agaaggcgac cacgatctga
      781 gcactcagat aggtcaccgc caagcaggcc aagaacaacg ggatcgccgc aacgaccgaa
      841 gccgccacac gggtcgtcac cagatagcga atcgggttta tgcccagagt gtcgagcgcg
      901 tcgatctctt cggcgatgcg catcgcgccc agctgcgcgg taaagcggca ccccgcttgg
      961 gcggtgaacg ccaacgcgag catgacgggt gcgagttcgc gtgtcgaccc ccacgacgcc
     1021 agcacgccgg ttgcaggacc taagcccaac agattcagcg cgttgtagcc ctcaacaccg
     1081 acgagcgcgc ccgcggtcac acccaacacc agtgcgacgc cgactgtgcc gccaccaacc
     1141 acgatcgagc cgttccccca ggcgatatcc gacagtatcc ggaagaactc tcggggatag
     1201 gaacgcagca taagcgggat cgaggtcacc acacgaccga cgaagtacat gatgtggccg
     1261 gctttggcca cggtccgcat taccgcatcc gctgcatcaa cgaacgcgcg agtgccccac
     1321 acgcgatatg aagcgaccgt cacggctaca gggtcgctct cggaaagatg aggatgtaga
     1381 gcttgctgat cgcgacattg accagcatca acaacaagat cgcctcgacc actgcggcgt
     1441 tcaccgagtt cgccactcca gcagggccac cacgggtttc caatcccttg taacagctga
     1501 cgattgcgac gatcgcgccg aacaccatcg ccttaatcag ggccacgacg agatcctgcg
     1561 gggtggcgaa tgcggcgaac gtcgcgacaa acgatccggg cgtcccgttc tgcatgtaga
     1621 cgttgaacac gtagcccgcg atgaatccga cgaagcatgt caaaccggtc aacgcaatcc
     1681 cgatcacgat caccgcggcg aaccgcggca cgaccaggcg tcggatcacc gatacaccca
     1741 tcacctccat cgcatcgatc tcttcgcgca tcgttcgcga acccagatcg gcgctcaccg
     1801 ccgagcccac cgcggcggcc agcaggatcg ccgccaccaa cggtgccccc tgacgcagca
     1861 caaacagtcc ggtggcggcc ccggccagcg aggtggcccc gatttgcccg gccagcaggc
     1921 tgaactggat tgacaacgtg gctgcgatcg ggagcgtcac cagaagcgtt ggaacaaaag
     1981 ctgttccggc catgaacgct gcctgctgga cgaattcgcc gaccatgaag cggccggtgg
     2041 ccaagtcggt aaagaagtat tgtgcggtgc gcacgcccaa caccacctgg ccacccatcg
     2101 tgtgcagcga cagcagcgga tgcgcccgta tgtagtggcg accccatccg cggatctctt
     2161 cgcgcgccga aggcgcctca actggagggt gacttgcgat taccgtagat tcgtccgccg
     2221 tcagcgatcc tgcgtcttgc cgatcggcgc tacgcagtgc catttcgtct gcgtaaccgg
     2281 atgtcggcat aggcaacgtc gtcgtggtgc tgatcgatcg accttcactg acacggtatg
     2341 cattcgggcc gtgctttcgg ctgatattca ccgaacttca agctggcccg acgattggca
     2401 tgccgctgtt aacagcgcga cggcgcgcac gccagagtgt gggcaagagt cgattgaaag
     2461 atctctcctt gcacgggcgc aaacgcgggg ttggccgtgc cgtccgttcg accggcgaat
     2521 ccgaaccgac cgccagatcc atgtcactgt gatgcacgcc tcaacaatcg cggtcggcgg
     2581 gcgcgtgcca cacgggtgta tctacctgac cccatacctg ttggcctacc catctttcgc
     2641 cttggtcgca tccgtttgac cgcttgctgc atagcgtggc gccgagaaac gcagcggctg
     2701 catctgtggc cgcatagggc aaaccgaagt gatcacacga acgacagcaa ggcatgacga
     2761 aaattctggg acggcaagcg ggctctggtg acaggtggaa gccgcggcat tggtcgcgcc
     2821 atcgcggaag cgttcgtctc agcgggagcg cgcatggtta tcgccgacat cgaggggtcc
     2881 gtggccgcgg ccgaggagat cggccgcggc acggtcggcg tggcatgtga cgttgcctcc
     2941 agcgagggcg ttgcggccgg gatcgctgag gccgtgcgag cgctcggcgg ccttgacctc
     3001 ttggtgaaca atgccggcgt gggatccctt gcgccgctgc acgaattgac cgaagaggag
     3061 tttgaccggg ttgtcgcggt gaacctgcgg ggcacctggt tgatgtacaa gcacgccgtg
     3121 cccgcgttgg tcgacggcgg cggggcgatc atcaacattg cgtccgtttc cggcttggtg
     3181 gggttcccgc tgctgggcgt ctactcagcc accaaaggcg ctgtgatccg gatgacggaa
     3241 acgatggcgc tggagttgcg cgaccacggc gtgcgcgcta acgcgatgtg cccggggttc
     3301 gtcgacaccg acatggttgc gcgctcggcc atcggtttca ccgcggccac cggcatgccg
     3361 ttcgaggaat ttgtcgccag gggccagggg cggcttgcca agcctgatga gatcgccggc
     3421 ctcgcggtct atttggcctc ggatgaggcg tcgttcgtca acggcgccgc gatcgccatc
     3481 gatggtggaa taaacgtgcg gcgcttctga ccgataggtg gttcgtgcca gcgcgtcaat
     3541 attacgacgc gcggcgtgcc atccagactg ccacttgggc ccgagagctg aatccgagtt
     3601 tggcgagaat gcgctcgacg tggccttcgg cggtgcgctt ggagatgacc agacgctccg
     3661 cgatctcacg attcgacagg ccgctctcga tcaactcggc aacttcgagt tctcgcttgg
     3721 tcaacagcgc tgcatcatct gtgcgccctg gtccgacgcg gtcacccccg acaccaagta
     3781 cgaaatcgat cacgtcgtcg agatcacgga accgatgcgc gacgtgacct tcatcgggca
     3841 cgcgagctgc gcgccgaggt ggcgcatgcc cttcagcgaa ccgagatagc tgcggcccga
     3901 acgcttcgat ggacgtgcct aatgttcgcc acacatggcg ggcagccgcc gacagggcct
     3961 cggcatgggc aacttgtttg cggtcatagg cgatccacga cagcaactcc gtggtgagtg
     4021 cgactccgat accgtcggcc attgtccgat gggtcttcag cactttgcgg gcggatcgtt
     4081 ccgtctcatc gaggtcaccc aacgtccagt gggcgacccc ggccgcccac tgcgcgtagg
     4141 cgcgcgcgtt ccgttctccg ttttcatcgc acaacgccac cgtttcgctg ctgatcgtca
     4201 gcgcatcctc ggcgtggcca ccgaccatga gcgcggtggc gagcgcgtag cgggcggtta
     4261 gttccatttc acgatctccg cgggcacggt acccgtcgac ggcgacccga agtccgcgaa
     4321 cggctccgtc cacatcaccg gtgaacatgg ccagtaaccc cgtccagtga tacacgtgca
     4381 ggtccagcct ggggtcatcc gactgcttcg cgagaactgc gagctcttcc agccatcgtt
     4441 cggtctggtg ccggtcacct tggacaagcg caatccagct cgccacccac aggcattcgg
     4501 cgcgcgccgc ggacggctcg cctaccgccg ccagcatcgt ctcgattcgc atacggcctt
     4561 cggcgaggag tccgccggat agccagtggt atcgcagcgc ggccaatagc aggagcccgg
     4621 tctgctcctc accttctgtc gcggcgctcc actgcagggc ggcgacatag ttggcgtgat
     4681 caaggcccat gcgggcgatc agcacatcct gctgcgggcc gcaccattgc tgcacgacct
     4741 gctgggcgcg ccgcaacatc gcgttgtggt gccggcgctg cagttcggcg gtttcgccgg
     4801 cctgctcggc gagctcgccg gcaacctccc gcaacggggc gagcagtcgg tatcgcattg
     4861 cacctgcggc ctggccggtc agaaggatcg attgcccgac caatcgctcg atggaatcga
     4921 tcacctcgtc gggatctagc tcgccgaagg cgcacaccga ctcggccagc tcgaggtcaa
     4981 aggttccggg gaacaccgac agacggcgcc acagtacttg cgcgttatac gggcagagtt
     5041 cgtagctcca gtcc
//
LOCUS       DASRJA010000512         6525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_582471,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000512 DASRJA010000000
VERSION     DASRJA010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582471"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VFC01_22375"
     CDS             complement(<1..406)
                     /locus_tag="VFC01_22375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZN82399.1"
                     /translation="MSAWRVQRPGPMTTHPLDRVTVDMPRPGPGELLVAVHACGVCRT
                     DLHVAEGDLPVHRPGVIPGHEVVGEVAAIGPDTGDEFAIGDRVGIAWLRHACGQCKFC
                     VRGQENLCPQSRYTGWDADGGYAEFATVPAAFA"
     gene            complement(460..762)
                     /locus_tag="VFC01_22380"
     CDS             complement(460..762)
                     /locus_tag="VFC01_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZN82400.1"
                     /translation="MDVLAATEFLARSTTLTSVGWIGYIIIGAIAGWIAGKIVKGGGS
                     GILMNIVIGIIGALIGGFLLSFWFDTAEGGWWFTLFTAILGSVILLWIVGMVQRKT"
     gene            complement(1055..2038)
                     /locus_tag="VFC01_22385"
     CDS             complement(1055..2038)
                     /locus_tag="VFC01_22385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine and proline-rich secreted protein Apa"
                     /protein_id="HZN82401.1"
                     /translation="MDQPDATPDYRKGLSTTLAIAALTGATMVALTLPVANADPTTPP
                     PPPPPAGNTFLNGQPPPEAPAPPPPADPNAPPPPPADPNAPPPAADPNAPAPPPPEPG
                     RVDNAAGGFSYVVPAGWKVADATQLSYGQALLNKIPAEATQPNGQPAQPPSDTSVLLG
                     RLDLKLFAGAETDNTKAAQRLASDMGEFFMPFPGTRVNQQTVPLNAGGMDGAASYYEV
                     KFTDANKPVGQIWAGVVGAPTPPGTPRGQRTPERWFVVWLGTANNPVPKNEAVTLAQS
                     IRPWTPPPPPPPDPNAPPPPAGGTNAPGQSSNVGVPVPVDPNSAPGMLPPG"
     gene            complement(2261..3319)
                     /locus_tag="VFC01_22390"
     CDS             complement(2261..3319)
                     /locus_tag="VFC01_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN82402.1"
                     /translation="MTGVALRAVVERRGVDIEFDIAAGEVLAVLGPNGAGKSTALHVI
                     AGLVRPDRGEVRVGQRVLTDTEAGVHIPTHDRRVGLLLQEALLFPHLNVARNVAFAPR
                     SSRAAAAHWLAEVDAAELADRMPRQLSGGQAQRVALARALAAEPDVLLLDEPLAGLDV
                     AAATAMRKVLRRVLARDGRSAVLITHDLLDVLTLADRVLVLEDGKIVESGSTATVLTT
                     PRSRFGARFAGVNLVGGTADAAGTLTTQWGTTWHGSPGPDMVAGESAVALFNPAAVAV
                     YRDKPHGSPRNTVEVTVAELDSRGAVIRVRADEQPDGSPGLAADITAESAAELRLAPG
                     DRVYFSVKAQEVTVHLSP"
     gene            complement(3326..4105)
                     /locus_tag="VFC01_22395"
     CDS             complement(3326..4105)
                     /locus_tag="VFC01_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN82403.1"
                     /translation="MLPRWIYLPAAIGALFVVLPLIAVAAKVDWPHFWSLITSESSTT
                     ALLLSLKTSAASTALCLLFGVPMALVLARSDARFVRMARPLILLPLVLPPVVGGIALL
                     YAFGRLGLIGEYLDAAGIQIAFTTTAVVLAQTFVSLPFLVIALEGAARTAGADYEVVA
                     ATLGARPTTVWRRVSLPLLAPGLISGAVLAFARSLGEFGATLTFAGSREGVTRTLPLE
                     IYLQRESDADAAVALSLLLVAVAAVVVVGLGSRRARGIDAW"
     gene            4244..4915
                     /locus_tag="VFC01_22400"
     CDS             4244..4915
                     /locus_tag="VFC01_22400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN82404.1"
                     /translation="MEVLVTGGDTDLGRTVAEAYRDAGHRVVIAGARRDDLEVAAKEL
                     DVDAIVFDNTDPASLEEVRGQLPHHLDTIVNIPAPRFDGGDPRTYSLADLAGAWRNAF
                     DATVVSAVLTVQILGDHLRSGGSIVNVITDQPREGSAEAAVKAAVSDWTAGQATHFGT
                     RGVTVNAVASGRNAEPGYDGLSRTPAPVAAEIARLALFLSTPAARHITGQTLHVSHGA
                     LANFG"
     gene            4981..5910
                     /locus_tag="VFC01_22405"
     CDS             4981..5910
                     /locus_tag="VFC01_22405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN82405.1"
                     /translation="MTIKLGLQIPNFSYGTGVEELFPTVIAQAQEADAAGFDSVFVMD
                     HFYQLPGLGTPDEPMLEAYTALGGLATVTKNVQLGTLVTGNTYRNPTLLAKAITTLDV
                     ASQGRAILGIGTGWFELEHDQLGFEFGTFTERFNKLDEALQIILPMLRGERPTFEGKY
                     YRAKEAMANPRFRDHVPLMIGGSGEKKTIPLAAKYFDHLNIIAGFDELPRKVAVVKDR
                     CEEIGRDPATLETSMLVIAVIDEKVTSDLIPDDFKQQAVVGSAEQVADQIKTKVLDAG
                     VDGVILSPITSLNGYQPGGITAVAEKLRPLLSA"
     gene            6021..>6525
                     /locus_tag="VFC01_22410"
     CDS             6021..>6525
                     /locus_tag="VFC01_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN82406.1"
                     /translation="MSHPGATATDRHRVVIIGSGFGGLNAAKALKHADVDIKMIARTT
                     HHLFQPLLYQVATGIISSGEIAPPTRIILRKQKNVQVLLGNVTHIDLASKTIKSELLG
                     HTYVTPYDSLIIAAGAGQSYFGNDHFAEWAPGMKSVDDALELRGRILGAFEQAERSSD
                     PARREKLL"
BASE COUNT         1151 a         2176 c         2175 g         1023 t
ORIGIN      
        1 gtgcgaacgc cgctgggaca gtggcgaatt cggcgtagcc gccgtcggcg tcccagccgg
       61 tgtagcggga ctgcgggcag agattctcct gcccgcggac gcaaaatttg cactgtccgc
      121 acgcgtgccg cagccaggcg atgccgacgc ggtcaccgat cgcgaactcg tcgccggtgt
      181 ccggcccgat agcggcgacc tctccgacca cctcgtggcc cgggatcacg cccggtcggt
      241 gtacaggcag atcgccctcg gcgacatgca gatcggtgcg gcacacaccg caggcgtgca
      301 cggcgacgag gagttcaccc ggccccggcc gcggcatatc gacggtgacg cggtccaacg
      361 gatgggtggt catcgggccc ggtcgttgga ctcgccaggc gctcatggtt gggacggaca
      421 ccctcctatg gtgctcgccc gtcgagagtt atgcgcccgc tacgtctttc gctgcaccat
      481 gccgacaatc cacaggagga tcaccgagcc gagaatcgca gtgaacaagg tgaaccacca
      541 gccgccctcg gcggtgtcga accagaagct gagcaggaag ccgccgatca gcgcgccgat
      601 gatgccgatc acgatattca tcagaatgcc cgagccaccg cccttgacga tcttgccggc
      661 gatccagccg gcgatcgcgc caataatgat gtagccaatc cagccaacgc ttgtcagcgt
      721 ggtggaccgg gccaggaact cggttgccgc aagtacgtcc atcgcagatc tcctgcctat
      781 cggccgtgcc cagcgggccg ctggaccacg cttgaaggta taccgcacgt gggtaacgat
      841 tcgggttctg aaatgggcac aaaccgcgag tccgagtacg gaactcgtag gaaaatcccg
      901 acgcccgatc gcacctgacg ggaactttca gcgctgccag tccgtcggcg tcctcgggtt
      961 cggcgggtcc atgccctgct tcattgtgaa cgtcaacaat ctgaacggcc agccgccggg
     1021 tgtgggtccg taagggccga ctgaacgttg gccgttagcc cggcggcagc attccgggcg
     1081 cgctgttggg gtcgaccgga accggcacgc cgacgttgga gctctggccg ggcgcgttcg
     1141 tgccgccggc aggtggcgga ggagcgttgg ggtccggtgg cggaggaggc ggcggcgtcc
     1201 acggccggat cgactgtgcg agtgtgacgg cctcgttctt cggcaccgga ttgttggcgg
     1261 taccaagcca aaccacgaac caccgctcgg gagtgcgctg gccacgcggc gtgcccggcg
     1321 gggtgggcgc tccgacgacg cccgcccaga tctggccgac cggcttgttc gcgtcggtga
     1381 acttcacctc gtagtacgac gcggcaccgt ccatgccacc ggcattgagc gggacggttt
     1441 gttggttgac acgggtgccg gggaacggca tgaagaactc acccatatcc gacgcgagcc
     1501 gctgggccgc cttggtgttg tcggtctcag cacccgcgaa cagcttcagg tcgagccgtc
     1561 cgagcagcac gctggtgtca cttggcggct gcgcgggctg gccattcggc tgggtggcct
     1621 ccgcggggat cttgttgagc aacgcctgtc cgtaagacag ctgcgtcgcg tcggccacct
     1681 tccaacccgc aggcactaca tagctgaagc cgccggcggc attgtcgacc cggcccggtt
     1741 ccggcggagg cggagcgggc gcgttcgggt cggcagccgg gggtggcgca ttgggatcag
     1801 caggcggagg cggcggtgcg ttcgggtcgg caggcggggg cggtgccgga gcctccggag
     1861 ggggctggcc gttcaagaac gtattgcccg ccggcggcgg agggggcggg ggcgtcgtcg
     1921 gatcggcgtt cgcaaccggc agcgtaagcg ccaccatcgt ggcgccggtc agcgccgcga
     1981 tcgccagcgt cgtcgagagg cccttgcggt agtcgggagt cgcgtccggc tgatccatgt
     2041 ggaagaaact accgtgttac gcccgtgacg caagtgcgag ttgctcaaaa agtgctcagc
     2101 tgagagattg ctgttaactg aaaagctgct gcgcgggggc cgatagtatg attgctgaca
     2161 tgacaaggcc cccgcgcgtc gcgcggggtg atcgaaccca ctggcacccc cgtggtttgt
     2221 ggtcgattca gaccgttttt cgcggtggcg aaccggaccg ttacgggctc agatggacgg
     2281 tgacctcctg agccttgacc gagaagtaaa cacgatcgcc gggtgccagc cgtaactcgg
     2341 ccgctgattc ggcggtgatg tcggcggcca gccccggcga gccgtcgggt tgctcgtcgg
     2401 cacgcacccg gatcacggcg ccgcggctgt cgagctcggc gaccgtgacc tcgacggtgt
     2461 tgcggggact gccgtgcggt ttatcgcgat acaccgcgac cgcggcgggg ttgaacagcg
     2521 ccactgccga ctcgcctgcc accatgtcgg gacccgggct accgtgccac gtcgtccccc
     2581 actgcgtcgt cagtgtgcct gcggcgtccg cggtgccgcc gaccaggttc actccggcga
     2641 accgcgcgcc gaagcgactc ctaggggtcg tcaaaaccgt tgcagtcgaa ccggattcga
     2701 cgatcttgcc gtcctcgagc acgagcactc ggtcggccag ggtcagcaca tcgagcagat
     2761 cgtgggtgat caacaccgca gagcggccgt cacgggccag cacgcggcgc agcaccttgc
     2821 gcatcgcggt ggccgcagcg acatccagtc cggccagtgg ctcgtcgagc agcaacacgt
     2881 cgggttcggc ggccagtgcc cgggccaacg ccacccgttg cgcctgcccg ccggacagct
     2941 gccgtggcat ccggtcggcg agctcagccg cgtcgacctc ggcgagccaa tgcgccgctg
     3001 ccgcacggga actgcgcggc gcgaacgcca cgttgcgggc aacgttgaga tgcggaaaca
     3061 acagcgcttc ctgcagcagc aggccgacgc gacgatcgtg ggtggggatg tgcacccccg
     3121 cctcggtatc agtcaggacg cgttgtccaa cacgcacttc cccgcggtcc gggcgcacta
     3181 ggccagcgat cacgtgcagc gccgtcgact tacccgctcc gttggggccc agtacggcaa
     3241 gcacttcgcc ggcggcgatg tcgaattcga tgtcgacacc ccgcctttcg acgaccgccc
     3301 gcagcgcaac cccggtcatg gccggttacc aggcatcgat cccccgcgct cggcggctgc
     3361 cgagtccgac gacgacgacc gcagccaccg cgaccaagag cagggacaac gccacggccg
     3421 cgtcggcgtc gctctcgcgc tgcagataga tctccaacgg cagagtccgg gtgacgccct
     3481 cacgggagcc ggcgaaggtc agcgtcgcgc cgaactcgcc aagcgagcgg gcgaacgcca
     3541 gcaccgcacc ggagatcagg ccgggcgcca gtagcggcaa cgacacccgc ctccacaccg
     3601 tcgtcggccg tgctccgagc gtggcggcca ccacctcata gtcggctccc gcggtgcgtg
     3661 ccgcgccttc cagcgcgatg accaagaacg gcagcgacac aaaagtctgc gccagcacca
     3721 ccgccgtcgt cgtgaacgcg atctggatgc ccgcggcgtc gaggtactcc cctatcaacc
     3781 cgaggcggcc gaacgcatac agcagcgcga tgccgccgac gacgggcggc agcaccagcg
     3841 gcaacaggat caggggccgg gccatccgga cgaaccgggc gtcgctgcgg gccagcacca
     3901 gcgccatcgg cacgccgaac aacaggcaca gcgccgtgct cgccgccgag gtcttcaggc
     3961 tgagcaggag cgcggtcgtc gacgactcgc tcgtgatcaa cgaccagaag tgcggccaat
     4021 ccaccttcgc ggcgaccgcg atcagcggca ggacgacgaa cagggccccg atcgccgcgg
     4081 gcagataaat ccagcggggc agcacaaatc acgaccctat ggcacgttga ctgaactgtt
     4141 gtcgtgtcgc atgcgcgccg agtttcgccg cctagctact gtccagtaac atgaccgtca
     4201 ctcgctgggg ttcagcgttg aacacaagca ggaggtcctt gccatggagg tgctcgtcac
     4261 cggcggcgat acagatctgg gacgcacggt tgccgaggct taccgcgacg cgggccaccg
     4321 agtggtgatc gcgggcgcgc gtcgcgacga tctcgaggtc gcggccaagg aactcgacgt
     4381 cgacgcgatc gtgttcgaca acaccgaccc ggccagcctc gaagaggtgc gcgggcagct
     4441 gccgcatcac ctcgacacca tcgtgaacat cccggcgcca cgctttgacg ggggcgatcc
     4501 gcgcacctat tcgcttgccg atctggccgg ggcgtggcgc aacgcctttg atgcgaccgt
     4561 ggtgtcggcg gtgctcacgg tgcagatcct cggcgatcat ctgcgctccg gcggttcgat
     4621 cgtcaacgtc atcacggacc agccccgcga aggcagcgcc gaagccgctg tgaaggccgc
     4681 cgtttcggac tggaccgccg ggcaagccac gcacttcggc acccgcggcg tcaccgtcaa
     4741 cgccgtggcc tccggccgca acgcggagcc tggttacgac ggcctgtcgc gcaccccggc
     4801 cccggtggcc gccgagatcg cccgcctggc gttgttcctg agcacgcccg ccgcgcggca
     4861 catcaccggg cagacactgc atgtcagcca cggggcgctg gccaacttcg gctgatattc
     4921 ccgcgttcgc ccgaagctaa acggggtgac cgccccgctt agttgtggtt acgtgaaggc
     4981 gtgaccatca aactcggact tcagattccc aacttctcgt acggcaccgg ggtcgaagaa
     5041 ctgttcccca ctgtcattgc ccaagctcag gaagccgacg cggcaggctt cgactcggtc
     5101 ttcgtgatgg accacttcta tcagttgccc ggcctcggga ctcccgacga gccgatgctg
     5161 gaggcgtaca ccgccctcgg cggcttggcg accgtgacca agaacgtgca gctgggcacg
     5221 ctggtcaccg ggaacaccta ccgcaatccg acgctgctgg ccaaggccat cacgacgctg
     5281 gacgtcgcca gccaaggccg cgcgatcctg ggcatcggca ccggttggtt tgagttggag
     5341 cacgatcagc tcggctttga gttcggcacc ttcaccgagc gcttcaacaa gctggacgag
     5401 gccttgcaga tcatcctgcc gatgttgcgc ggcgagcggc cgaccttcga gggcaagtac
     5461 taccgcgcca aggaggcgat ggccaatccg cggttccgtg accacgtccc gctgatgatc
     5521 ggcggcagcg gcgagaagaa gacaattccg ttggccgcca agtactttga tcatttgaac
     5581 atcatcgccg ggttcgacga gctaccgcgc aaggtggctg tggtcaagga ccgctgcgag
     5641 gagatcggcc gcgatccggc gacattggag accagcatgc tggtgatcgc cgtgatcgac
     5701 gagaaggtca cctccgatct gattcccgac gatttcaagc aacaggctgt cgtcggcagc
     5761 gccgagcagg tggccgacca gatcaagacc aaggtgctcg acgcgggcgt cgacggcgtc
     5821 atcctgagcc cgatcacaag cctgaacgga taccagccgg gcggcatcac cgcggtggcc
     5881 gagaagttga ggccgctcct ttccgcttga gtccgtttaa aaaccccggc ggcgtggcaa
     5941 atctcacggg gctgcgggga agcgggacgg ctaccgtagt gtctgtataa gtgcacgtgt
     6001 agtccgtcga ggagccgaag atgagccatc ccggagccac tgcaactgat cgacacaggg
     6061 tcgtgatcat cggttcggga ttcgggggtt tgaacgccgc caaggcgctc aagcacgccg
     6121 acgtcgacat caagatgatc gccaggacca cgcatcacct gttccagccg ctgctgtacc
     6181 aggtggcgac tggcatcatc tcgtcgggcg agatcgcccc gcccacccgg atcatcctgc
     6241 gtaagcagaa gaacgtgcag gtgttgctgg gcaacgtgac gcatatcgac ctggccagca
     6301 agacgatcaa gtcggagttg ctcggtcaca cctacgtcac gccgtacgac agcctgatca
     6361 tcgcggcagg tgccggccag tcctacttcg gcaacgatca cttcgccgag tgggcgcccg
     6421 gcatgaagtc ggtcgatgac gcactcgagt tgcgcggccg gatcctcggc gcgttcgaac
     6481 aggccgagcg ctccagcgat cccgcgcgcc gcgagaaact gctga
//
LOCUS       DASRJA010000513         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3752394,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000513 DASRJA010000000
VERSION     DASRJA010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3752394"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFC01_22415"
     CDS             <1..217
                     /locus_tag="VFC01_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HZN82407.1"
                     /translation="ARARKPDVYADAAYEVLNKPAREYTGKALLCEDVLLESGVTDLS
                     IYDCVPGGKLGVDLWVDTPNPPGYKGP"
     gene            complement(214..1377)
                     /locus_tag="VFC01_22420"
     CDS             complement(214..1377)
                     /locus_tag="VFC01_22420"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HZN82408.1"
                     /translation="MKCRGAVMHGVGQDWEIEEITLDPPREGEVLVKMAVAGICHSDD
                     HFSTGDMVPTPELAAIMESTGAAPLNYFPLLGGHEGAGVVAEVGPGVRSVQPGDHVSF
                     SFIAACGACRWCVSGMTYLCDNGAMMLAEEMTTDGTCRRHLGDENLTAMTQLGTFAEY
                     AVLAEESVIKIDDSIPLEAASLVSCGVTTGWGSGTVGVGTQPGDTVVIIGTGGVGINA
                     VQGARAAGANAVIAVDPVEFKRDTAKFFGATDTSPSVEEAIPLVRELTQGVMADRVVL
                     TPSVTHAELLAPALMLTRKGGTCLMTAVPKFELNMVPLLLVDMLQSCKTFKGLIYGGM
                     NPRASMPMLLDMYRNGHLKLDELITRRYRLDQINDAITDMREGRNIRGIIEFA"
     gene            complement(1472..1672)
                     /locus_tag="VFC01_22425"
     CDS             complement(1472..1672)
                     /locus_tag="VFC01_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82409.1"
                     /translation="MIEFALWMAIPYIVIGLAFAFFGAEQVQLIETQLQTRLPAGSEI
                     AAYLLTALSWPATLFGVDVCMA"
     gene            complement(1716..>2018)
                     /locus_tag="VFC01_22430"
     CDS             complement(1716..>2018)
                     /locus_tag="VFC01_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZN82410.1"
                     /translation="QIDEQVRAYLQDLLNLRMVNPVLALPFRPLLKFLTVGFLAPVFR
                     DAMGVQWSGFKQRQFEWLFLFVAFVNRFLPVFVRQGGSYVLLADVRRRARTHRALV"
BASE COUNT          393 a          657 c          614 g          354 t
ORIGIN      
        1 ggcccgggca cgtaagcccg acgtgtacgc cgacgccgca tacgaggtcc tgaacaagcc
       61 tgcccgcgag tacaccggca aggccctgtt gtgtgaggac gtgctgctgg agtctggtgt
      121 caccgacttg tcgatctacg actgcgtgcc cggcgggaaa ctgggcgtcg acctgtgggt
      181 cgacacccca aacccgccgg gatacaaagg gccctaggcg aattcgatga tgccgcggat
      241 gttgcggcct tcgcgcatat ccgtgatcgc atcgttgatc tggtctagcc ggtagcggcg
      301 agtgatcagc tcgtcgagtt tgagatggcc gttgcggtac atgtccaaca gcatcggcat
      361 gctggcgcga gggttcatcc cgccgtagat cagtcccttg aaggtcttgc acgactggag
      421 catgtcaacg agcaacagcg gcaccatatt cagttcgaat ttcggcaccg cggtcatcag
      481 gcaggtgcca cccttgcgcg tcagcatcaa ggcgggagcc aacagttcag cgtgggtcac
      541 gctcggcgtc agcaccactc tgtccgccat gactccctgg gttagttccc ggacgagcgg
      601 tatggcttct tcgacggatg gactggtatc ggtcgccccg aagaacttcg ccgtatctcg
      661 tttgaactca acgggatcca cggcaattac ggcgttggca cccgcggccc tggcgccctg
      721 cacggcgttg atccccaccc cgccggtgcc aatgatgacg accgtatcgc cgggttgcgt
      781 gccgacgccg acggtgccgg agccccaacc cgtcgtcaca ccgcacgaca ccagcgaggc
      841 agcctcgagg ggaatcgagt cgtcgatctt gatcactgac tcctcggcca gcacggcgta
      901 ttcggcgaac gtgccgagtt gcgtcatcgc ggtaaggttt tcatcgccga ggtgacgtcg
      961 gcaggtgccg tccgtcgtca tctcctcggc caacatcatc gccccgttgt cgcaaagata
     1021 tgtcatgccc gacacgcacc atcgacatgc cccgcacgcg gcaatgaacg agaacgagac
     1081 gtgatcaccg ggctgcacgg accgcacgcc gggaccaacc tctgcgacga cgccggcacc
     1141 ttcgtggcca ccgagcaggg ggaagtaatt cagtggggcc gcgccggtgg actccatgat
     1201 tgccgcaagc tcgggtgtgg gcaccatgtc gccggtggag aaatggtcgt cggaatggca
     1261 gataccggcg acggccatct tgaccagcac ttcgccctca cggggtgggt cgagggtgat
     1321 ttcctcgatt tcccagtcct ggccgacacc gtgcatcacc gcgccccgac atttcatttg
     1381 cgtcctcccc gtttcactgc gcagctggac ttgcgctgtg agtcagaatc ccactggccg
     1441 gacgcccgga tgcgggtatt cgcatactcg gtcaggccat acagacgtcg acgccgaaca
     1501 gcgtcgccgg ccacgacagt gccgtcagca gataggccgc gatttcggaa cccgcaggca
     1561 gcctggtctg cagctgcgtc tcgatcagct gcacctgctc ggcgccgaag aacgcgaacg
     1621 ccagcccgat gacgatgtac ggaatcgcca tccacagcgc gaattcgatc atcgcctcga
     1681 cactgacccg gcggctcagc acgcggcgca acacgtcaca ccagcgctcg gtgagtgcga
     1741 gcccgcctgc gtacgtcggc gagcagcaca tagctgccgc cttgacggac gaataccggc
     1801 aggaaccggt tgacaaatgc cacgaagagg aacagccact cgaattggcg ttgtttgaat
     1861 ccgctccact gcacccccat cgcgtcgcgg aacaccggag ccaggaaccc gacggtaaga
     1921 aacttcagca gcggccggaa cggcagcgcc agaaccggat tgaccatgcg aaggttcagc
     1981 agatcctgca gatacgcacg gacctgttcg tcgatctg
//
LOCUS       DASRJA010000514         3209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_17346,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000514 DASRJA010000000
VERSION     DASRJA010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17346"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFC01_22435"
     CDS             complement(<1..264)
                     /locus_tag="VFC01_22435"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HZN82411.1"
                     /translation="MRLVHPTQVRPERWANGGGWTRTLLAWPDPRRWSVRVSVADVET
                     AGPFSAYPGVDRWFAVLEGGGVRLTTAGRAPASVRADDDEMHAF"
     gene            complement(261..2768)
                     /locus_tag="VFC01_22440"
     CDS             complement(261..2768)
                     /locus_tag="VFC01_22440"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZN82412.1"
                     /translation="MNDPLAPFAPNDNDVQAQDMPNPVVIGGYVNVPRAGFDGRSFQN
                     GDIDDVYRVDLLAGQVIELVIPSADVGDDADLFLFDSAGNLVDSSEGTGQVETLVVAS
                     GGTYFIDVFAFEGAPMYRLSVGQSTVSSNTASLRLSDEFVPGELIVTLKNFAKSSSDA
                     AATESTSAATLATRFELSRKGGAPDRAMLLKLPADTTAITNGMTPWSRAAAAGTKSAT
                     ATAVTTAATGAAKTAAPRNWRVASALQQRKLDTLLYAKRLRSDAAVRSADLNRIMRTS
                     LTPNDANYPIQRWHYEQIQLPGAWDVTTGSTAVRVAVVDTGVVITHPELSSKLVQGFD
                     FVSSPSNEDGDGIDAIPADPGCVIGGGSTFHGTHVSGTIGARSNDGSGVAGVSWGARI
                     MPIRALDGCVGTGTSFDIIQGIRYAAGLENDSGTLPTQRADVINMSFGAAGACDTSAS
                     DLFAQVRAQGVAVVAAAGNDNSSGPQSPASCDNVISVAATGPLRTKAPYSNFGSTVDL
                     AAPGGDMRVDVDGNGLPDGIYSTHANGGGANITPTLDHLQGTSMAAPHVAGVIALMLS
                     VNPAMTPAQFDALLAQGSLTDPIPGENRLGQGLINAAKAIGAANPSLPQPPPRISVTP
                     SSLAFGDIGTTAQVVVANAGGGTLTVTGANPSDPWISVVETSVDANGLGLYTITVDRT
                     GLPVGSFSGFVLFTSNGGTGRVEVLMEVAAVSGEPSAGKQYVLLVDPVTNDTLAQVEV
                     LAEGSSVAYQFDGLAARDYLLMSGTDLNNDQFICDPAEACGVYPVEAAPVTIAVDGAE
                     TGFDFVVSYRTGVPTSASSTGTKGVRKEVRQRRLIRK"
     gene            2836..>3209
                     /locus_tag="VFC01_22445"
     CDS             2836..>3209
                     /locus_tag="VFC01_22445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82413.1"
                     /translation="MVTVALAVPEPPAPLHSSVNVVAALIGPVVSDPDGDLTPLQPSD
                     AWQVSASVLDQVSVDVPPTTTFVGSAWSCTVGAGGGPLPPPPPPPPQPATSVATPARR
                     IFRGSMCGIVRVAGMSDFSLRGG"
BASE COUNT          480 a         1098 c         1092 g          539 t
ORIGIN      
        1 gaatgcgtgc atctcgtcgt cgtcggctcg cacggaggct ggcgcgcgac ccgcggtcgt
       61 cagccggacg ccgccgccct cgagcaccgc aaaccagcga tcgacacctg ggtatgccga
      121 gaacggtccg gccgtctcga cgtcggcgac gctcacccgc accgaccagc gccgcggatc
      181 gggccaggca aggagcgtgc gcgtccagcc gccgccattg gcccagcgct caggtctgac
      241 ctgcgttgga tgtaccagcc tcacttcctg atcagacgcc gctgccgtac ctctttccgc
      301 acgcccttcg tgcccgtcga gctggcgctg gtcggcacac cggtgcggta cgacaccacg
      361 aagtcgaacc ccgtctccgc cccgtcgacc gcgatcgtca ccggtgcggc ctccaccgga
      421 tagacaccgc aggcctcggc cggatcgcag atgaactggt cgttgttgag gtcggtaccg
      481 gacatcagca ggtagtcgcg ggcggccaga ccgtcgaact ggtaggcgac cgagcttccc
      541 tcggccagca cctcgacctg cgcgagcgtg tcgttggtca cagggtcgac gagcaggacg
      601 tactgcttgc cggcgcttgg ctcgccgctg accgctgcca cctccatcag cacctcgacc
      661 cgtcccgtgc cgccgttcga ggtgaagagg acgaaccccg agaagctgcc aaccggaaga
      721 ccggtgcgat cgaccgtgat cgtgtacagg ccgaggccgt tcgcatcgac cgaggtctcg
      781 accacggaga tccacggatc ggacgggttg gcgccggtca cggtgagcgt cccaccgccg
      841 gcgttcgcca ccaccacctg tgccgtcgtg ccgatgtcgc cgaaggcgag cgaggagggc
      901 gtgacgctga tccgcggagg cggctgcggg agcgacgggt tcgcggcccc gatcgccttg
      961 gccgcattga tcaggccctg cccaagtcga ttttctcctg ggatagggtc ggtgagcgat
     1021 ccctgtgcca gcagcgcgtc gaactgcgcg ggtgtcatgg cagggttcac gctcagcatc
     1081 agcgcgatca cgcccgcgac gtggggcgcg gccatcgagg tgccctggag atgatcgagg
     1141 gtgggcgtga tgttggcgcc gccgccattc gcgtgcgtgc tgtagatacc gtccggcagc
     1201 ccgttgccat cgacgtccac gcgcatgtcg ccgccgggcg cggccaggtc gacggtcgag
     1261 ccgaaattgg agtatggcgc cttggtccgc agcggccccg tggctgcgac gctgatcacg
     1321 ttgtcgcagg aggccgggct ctgcggtccg ctgctgttgt cgttgcccgc agcggcgacc
     1381 accgccacgc cctgcgcgcg gacttgtgcg aacagatccg acgcactcgt gtcgcaggcg
     1441 ccggcggcgc cgaagctcat gttgatcacg tcggcgcgtt gcgtgggcag cgtgccggag
     1501 tcgttctcca ggccagcggc ataacggatg ccctggatga tgtcgaaaga cgtgccggtc
     1561 cccacgcaac cgtcgagcgc gcggatgggc atgatccgcg cgccccagga cacacccgcg
     1621 acaccgctgc cgtcgttgct gcgcgcgccg atggtcccgg acacgtgcgt gccgtggaac
     1681 gtggagccgc caccgatgac gcagcctgga tcggcaggga tcgcgtcgat gccgtcgccg
     1741 tcctcgttcg acgggctgct gacgaaatcg aacccttgta cgagcttgga cgagagttcc
     1801 ggatgcgtaa tgacgacacc cgtgtcgacc acggcaacac ggaccgccgt gctaccggtc
     1861 gtgacgtccc acgcaccggg caactgtatc tgctcgtaat gccagcgctg gatcggataa
     1921 ttggcgtcgt tcggcgtcaa cgacgtgcgc atgatccggt tgaggtctgc gctgcgcacc
     1981 gccgcgtcgc tgcgcaggcg ctttgcgtaa aggagcgtgt cgagcttgcg ctgttgcagg
     2041 gcgctggcaa cgcgccagtt ccgcggcgct gctgtcttgg cagcgccggt cgctgcggtc
     2101 gtgacggccg tcgcggtcgc gctcttcgta ccagccgccg cggcgcgcga ccagggtgtc
     2161 atgccgttcg tgatggccgt cgtatcggcg ggcagcttga gcagcatcgc acgatcgggc
     2221 gcgccgcctt tccggctcag ctcaaagcgc gtggcgagcg tcgcggcgct tgtcgattcc
     2281 gtcgcggccg cgtcactgga gctcttggca aaattcttga gcgtgacgat cagctcgccg
     2341 ggcacgaact cgtcgctcag acgcaggctc gccgtgttcg atgacaccgt cgattgcccg
     2401 acgctcagcc ggtacatcgg tgccccctcg aaggcgaaca cgtcgatgaa gtacgttcca
     2461 ccggaggcaa cgaccagcgt ctcgacctgg cccgtaccct ccgacgagtc gacgagattt
     2521 ccggcggagt cgaaaaggaa caggtcggcg tcgtcgccga cgtcggcgga cgggatcacc
     2581 agctcgatca cctgcccggc gaggaggtcg acgcggtaga cgtcgtcgat atcgccgttc
     2641 tggaacgacc ggccgtcaaa tccggcgcgg ggcacgttca cgtagccgcc gatcaccacc
     2701 gggtttggca tgtcctgcgc ctgcacgtcg ttgtcgttcg gtgcgaacgg cgccagcgga
     2761 tcgttcacgt cactgtcgga gcgcgaagtc gtcggcacca gcaggttgcc ggctaccgag
     2821 ctgttgcctg cctgtatggt gacggtcgca ctcgccgtgc ccgagccgcc agcgccattg
     2881 cactccagcg tgaacgtcgt ggccgcgttg atcggcccgg tggtttccga cccggacggc
     2941 gacctcacac cgctccagcc gtcggacgcc tggcaggtgt cggcatcggt gctcgaccag
     3001 gtgagcgtcg atgtaccgcc gacaaccaca ttcgtcgggt ccgcctggag ctgcacggtg
     3061 ggtgccgggg gcggcccgct cccaccgccg ccgccgccgc ctccgcaacc ggcgaccagc
     3121 gtggcgaccc ccgcgaggag gatcttccgt ggctcgatgt gcgggatcgt cagggttgcc
     3181 ggcatgtccg atttctctct gcgcggtgg
//
LOCUS       DASRJA010000515        12199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_24280,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000515 DASRJA010000000
VERSION     DASRJA010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12199
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24280"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VFC01_22450"
     CDS             <1..948
                     /locus_tag="VFC01_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HZN82414.1"
                     /translation="FDILVCTTIVETGLDISNANTLIVDRADTFGLSQLHQLRGRVGR
                     SRERGYAYFLYPPEVPLTETAYDRLATIAQNNELGAGMAVAMKDLEIRGAGNVLGVEQ
                     SGHVAGVGFDLYVRLVGEAVEAYRAAADGKTVAAPEEPKDVRIDLPVDAHLPPDYIGS
                     DRLRLEAYRRLAAAADDAAVDSVIEELVDRYGPPPQAAQRLVAVARLRLLFRHYGITE
                     LSAVSESTLRLSPLQLADSQQLRLKRLYPGAHYRATTSTVQVPIPRAGSGVGAPRIRD
                     LELVAMVAGLVLALDGKSQEGVDITKFGGSAMSSERQAT"
     gene            945..2114
                     /locus_tag="VFC01_22455"
     CDS             945..2114
                     /locus_tag="VFC01_22455"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZN82415.1"
                     /translation="MTVVLVDPRRPSLVPVEAIDLLTGDVQYTEEMPVKVPWSLPAAR
                     PAYDGEDAPVLLSSDRDHPAVKARLAAGDRLIAAPDAAPGERLVDAVAMMDKLRTSGP
                     WESEQTHDSLRRYLLEETYEVFDAVRGGNADELREELGDVLLQVLFHARIAEDAPQHP
                     FTIDDVADSLVRKLGNRVPAVLAGESISLDEQLAQWEERKALEKSRNSSMDDVPTGQP
                     ALALAQKVLERVAAVGLPADLIPDTITSVTVAAGSDGENALRSAVLEFMDTVRQAEHA
                     IAAGRRGEDVPEELDVATLGEISEEEWRAYWPTTTEDTEPAPDRIPEPTPADVPERGP
                     VVGPAPEESGVPEPAPVEVPETEPAPEDVSTEEALADESDEDVDDSEHAAEKFSA"
     gene            2243..2521
                     /locus_tag="VFC01_22460"
     CDS             2243..2521
                     /locus_tag="VFC01_22460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82416.1"
                     /translation="MKLVPANIEQMANRVDRQPSLAIGISSGLLAFWSIYRVFWSLYL
                     ALTYDFLFGSLIFQVVLWGVIGAVAAIASIAFLTRYAKPADTTEHNQP"
     gene            complement(2714..3286)
                     /locus_tag="VFC01_22465"
     CDS             complement(2714..3286)
                     /locus_tag="VFC01_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HZN82417.1"
                     /translation="MAGFVLFLLPWLGAGFVPDPAAAWTAWVVGFLAMALGAIGWLSS
                     RPPTIYGINENTSSQARPSAVADWISRAALVIGLGTVVLGATVAQSSPAAVAVTVGLG
                     GFTAVIAMWSMLAADPTRDYFTMAVVGFALFLAPWLAGFAGNEAAWAAWIPGSIATAL
                     GVAGYLRGESLDFARTVRSDADARYRQRFG"
     gene            complement(3659..4450)
                     /locus_tag="VFC01_22470"
     CDS             complement(3659..4450)
                     /locus_tag="VFC01_22470"
                     /inference="COORDINATES: protein motif:HMM:NF039955.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5994 family protein"
                     /protein_id="HZN82418.1"
                     /translation="MTDRPILPCSTATKTGPAVVERRQASHANSYHHGRYPPSFTVVA
                     VGDIERNDGEPPCARQPWHPNTTRREHTSHNKGRQHMLTINENIPDASVRRPALGALR
                     MRLKPAHRSCGFVQGAWWPRSTELASEIPSLLAALSLRFGSIDSVLYHESDWSPAPLS
                     IKHQGEQVIVSAHQEWPNVVSVFGPRFGRLDLLVVPPYTEPTHAYNVVTAAASVNDAS
                     TPDQLLGITRRADDRLLSPIALERWESDGGAVPPAPWSQLEMQDA"
     gene            complement(4612..4986)
                     /locus_tag="VFC01_22475"
     CDS             complement(4612..4986)
                     /locus_tag="VFC01_22475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82419.1"
                     /translation="MSPTAPTFATLNDELNKAHHRIRELQAREAELLAQNRTLCAVIT
                     ELTGEDPARSAAELCSSALQASPSNDGSDDRFGIGDHVGEQSGQRLGPAARPPVPANT
                     PAPVSASGTGTARVKPRQFPYV"
     gene            complement(5039..5521)
                     /locus_tag="VFC01_22480"
     CDS             complement(5039..5521)
                     /locus_tag="VFC01_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HZN82420.1"
                     /translation="MATTTRVWISPQAHERLHRELDTLRFLFSAGIADEGTDENATAV
                     RRAWETRMQQIHDLLINAVVGEDPPNDGIAEPGMVVTIRRDATGDTETFLLGVHGAEF
                     ADMPVYSIESPLGAAITGARAGERRTYRLPNGTPLAVTLLKAVPYGLHIADLSQPAPQ
                     "
     gene            complement(5806..6261)
                     /locus_tag="VFC01_22485"
                     /pseudo
     CDS             complement(5806..6261)
                     /locus_tag="VFC01_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014384357.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
     gene            complement(6396..7661)
                     /locus_tag="VFC01_22490"
     CDS             complement(6396..7661)
                     /locus_tag="VFC01_22490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN82421.1"
                     /translation="MGGGERIDGGPRSAIVVGAGIVGLSTAWFLQERGVDVTVVDRTG
                     VAAGASWGNAGWISPALTLPLNSPGVLRYGLRALLDPAAPLHIPMTADSALGVFLTQF
                     VANCRRSSWKRAVRANVPLNEEAIEAFDVLVANGVDAPVTDAPITALFRSTEQAERMM
                     RELRALENAGQTMSVTALSGEALREQVPLASPAITAALSINGQRFVDPGRFVHALGQA
                     VADRGATMRTAEVRSVFRSGSGITVYPRSGMPLTADTAVIATGAWLSRLAGSRVRVPV
                     QAGRGYSFTVPVDRPIPGPIYLPDVRVACTPYHGALRVSGTMEFRDPHEPAVPARVGA
                     IVASASPLLDGVRWAERSDIWVGPRPVTPDGRPLIGEMSRGVYVAGGHGMWGLAHGPV
                     SGRLLAEQITTGKQPEALRDFDPLRRTGR"
     gene            complement(8430..8942)
                     /locus_tag="VFC01_22495"
     CDS             complement(8430..8942)
                     /locus_tag="VFC01_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5994 family protein"
                     /protein_id="HZN82422.1"
                     /translation="MSAPPLSFDISQARRWTITHPRLRLKPAHRSAGVVQGGWWPLTD
                     QLYVELRFLLSALSSGSGTIERVIYDENSWASASLRMEFRGHSVILEPSNTSPNTLTV
                     SGKKFGTLVLLVVPPDTDPVAAHTAVMTAADPDDVSTAEELLAIGMRAAQEARPASRT
                     DHHGGSDGEM"
     gene            complement(8999..9595)
                     /locus_tag="VFC01_22500"
     CDS             complement(8999..9595)
                     /locus_tag="VFC01_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82423.1"
                     /translation="MDTFTVRLPRWPVLLRLAGRDPLVRTIDRIEALVLVLAVVVSLL
                     AAPIAAAVGTAVYDSSRHIYAEQAHIRHTVAATVTDVPASQQILRTGTTTVSARWTAA
                     GAEHTGTVKAQSTAKAGDTLEIWVDDNGSQVPAPAPTSRAAAEAAMGALVIWICVAAI
                     AATLFTLTRAVCDRIRFTRWQHDLDNLVDNDGHKPSQP"
     gene            complement(9876..11405)
                     /locus_tag="VFC01_22505"
     CDS             complement(9876..11405)
                     /locus_tag="VFC01_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82424.1"
                     /translation="MVTLDRLVNVLGSYGVRLRFCPIPRSTELRSVVMHERTEDRTVV
                     GDVLLGIGARSVKEAVRWAASARAVVVLIREGDDDDTAFSGAAEGVAVMVVDPAMTWS
                     ELAAVIYGLVLEGRETESGRGPTDLFALADSLAEASGGAVIIEDRLSRVLAYSRRQQD
                     ADPARVATIVERQAPEQLRAFFDARGVYTHLAASDAPVFVTRDPDHGMTGRMVVAVRS
                     GRELMGSVWVECRAPLKGAALTALADGARTVALHLLRSRASADLERQVESELVIRLLE
                     GTADAATAASRLGLPHTLLRVIAVQAFIGAERDAALLLAFERATTGFGWSRPGRSALA
                     GNTVYTLLPGEQVDTARRWVTGLRAALPERMAVLAGISGPAEVADLVAARHEADECLA
                     LHEIWPTGATPPAYDESWDDILLQRLRTAARSGRSPDRGPVAELRRHDRDHTTDYVPT
                     LRAWLEAQGDPTEAGNRLGVHENTVRYRLRKMAEITNLQLDDAKKRLAMMIELAATDT
                     D"
     gene            complement(11559..11696)
                     /locus_tag="VFC01_22510"
     CDS             complement(11559..11696)
                     /locus_tag="VFC01_22510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82425.1"
                     /translation="MGDHLVIPSTRHGLHAVDACVVLLTVAKTVQRYGERAYDDDLPG
                     L"
     gene            complement(11714..12001)
                     /locus_tag="VFC01_22515"
     CDS             complement(11714..12001)
                     /locus_tag="VFC01_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82426.1"
                     /translation="MTVHQPALEALGDHDYLVRFQQNEDTVVVRMHADPAVVAQIAAE
                     EQRVVEATAAYLIARQNADDLPEQVDLDTVAAAYDGYVADLHRQLTPPSGH"
     gene            complement(12035..>12199)
                     /locus_tag="VFC01_22520"
     CDS             complement(12035..>12199)
                     /locus_tag="VFC01_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82427.1"
                     /translation="AVQLLVVRPRLNRRSARVLAGHDAPRSRAHHAYIGLEALKLAAL
                     VALGAALLAG"
BASE COUNT         1987 a         3752 c         4251 g         2209 t
ORIGIN      
        1 ttcgacatcc tggtgtgcac gacgattgtc gagacaggac tggacatttc gaacgcgaac
       61 acgttgatcg tggaccgcgc cgacacgttc ggactgtcac agctgcacca gttgcggggc
      121 cgggtcggcc gcagccgtga gcgtggctac gcgtatttcc tgtatccacc cgaggtgcca
      181 ttgacggaga cggcctacga ccggctcgcg acgatcgccc agaacaacga gctgggtgcc
      241 gggatggcgg tcgcgatgaa agacctcgaa atccgcggcg ccggaaacgt gttgggcgtc
      301 gagcaatccg ggcacgtcgc cggcgtcggc ttcgacctct acgtgcggct ggtcggcgag
      361 gcggtcgagg cgtaccgcgc ggcggccgac gggaaaaccg ttgcggcgcc tgaggaaccg
      421 aaggacgtcc ggatcgatct tccggtcgac gcgcatctgc cgccggacta tatcggcagc
      481 gaccggctgc ggctggaggc ctatcggcgg ttggcggcgg ccgcggacga cgctgcggtg
      541 gactcggtca ttgaagagct tgttgaccgg tacggcccgc cgccgcaggc cgcccaacgt
      601 ttggttgccg tggcgcggct gcgcttgttg ttccgccact acggaatcac ggaactcagc
      661 gcggtatccg agtcgacgct gcggctgtcg cccttgcagc tggccgactc gcagcaacta
      721 cgactcaagc ggctgtaccc gggcgcgcat taccgtgcga cgacgtcgac ggtgcaagtg
      781 ccgatcccgc gcgcgggttc tggcgttggg gcaccacgga ttcgtgatct tgaactggtc
      841 gccatggttg ccggtctggt gctggccctc gatggaaagt cgcaggaagg tgttgatata
      901 acgaagttcg gaggttcggc aatgagtagt gagcgacagg cgacatgaca gtcgtcctgg
      961 tcgaccctcg ccgcccttcg ctcgttcccg tcgaggcaat tgacttgctg accggtgacg
     1021 tccagtacac cgaggagatg cccgtcaagg tgccgtggtc gctgcccgct gcgcgcccgg
     1081 cctacgacgg ggaggacgct ccggtgttgc tgtcgtcgga tcgcgaccat cccgcagtga
     1141 aggcgcgcct ggcggccggc gacaggctga tcgcggcgcc cgacgcggct ccgggggagc
     1201 ggctggtcga tgcggtggcg atgatggaca agctgcgcac gtcgggcccg tgggaaagcg
     1261 agcaaacaca cgactcgctg cggcgctacc tgctggaaga gacctacgag gtgttcgacg
     1321 cggtccgcgg tggcaacgcc gacgagctgc gtgaggagct cggagacgtg ttgctgcagg
     1381 tgctgttcca cgcacgcatc gcggaggatg cgccgcagca tccgttcacc attgacgacg
     1441 tagcggattc gctggtgcgc aagctcggca accgagtgcc cgccgtgctc gcgggtgagt
     1501 cgatctcgct cgatgagcag ctcgcccagt gggaagagcg caaggcactc gagaagtcac
     1561 gcaactcgag catggatgac gtgccgaccg gacagcccgc attggcgttg gcgcagaagg
     1621 ttcttgagcg agttgcggcc gtggggctgc ccgccgatct gatccccgac accatcacat
     1681 cggtgacggt cgccgctggt tccgatggcg aaaatgcttt gcgctcagca gttttggaat
     1741 tcatggacac cgtgcgccag gccgagcacg ccatcgcggc ggggcgtcgt ggtgaggacg
     1801 tgccggagga actcgacgtg gcgacgctgg gcgagatctc cgaggaggag tggcgggcct
     1861 actggcccac cacaaccgaa gacaccgagc ccgcgccgga ccgcatcccg gagccgacgc
     1921 ccgccgacgt gcccgagcgg ggaccggtgg tgggccccgc tccggaggag tcgggagtgc
     1981 cggagcccgc gccggtggaa gtgcccgaga ccgagcctgc gcccgaagac gtctcgacgg
     2041 aagaggccct cgccgacgag tccgacgagg acgtcgacga cagcgagcac gccgccgaga
     2101 agttcagcgc gtaaccgcgc acgaaaatta ggaaaattcc cgcttcattg cccgccattg
     2161 ttgggatcgg tagccaaaca cgcgcgctgt actaacggca acggcgccaa ccagacggcg
     2221 ccccgtcaaa ggacaacaga caatgaaact tgtacccgcc aacatcgagc agatggccaa
     2281 tcgcgtcgat cgccaaccca gcctggcgat cgggatcagc agcggcctgc tcgccttctg
     2341 gtccatctac cgcgtgttct ggtcgctcta cctcgcgctg acgtacgact tcctgttcgg
     2401 gtcgctgatc ttccaggtag tgctgtgggg tgtgatcggc gccgtcgccg cgatcgcttc
     2461 gatcgcgttt ctgacccgct acgccaaacc ggccgacacc actgaacaca accaaccctg
     2521 aaccacggtt ccctgcccgc ctcacgggcg acattgtcgc tactgacgac tttgtcgcca
     2581 cgaggcgggc acttgtcggg tctcaaactt cgtggcgaac ggcgatgccc tgaccagcga
     2641 tttgtcgtcg tctcactgat tggcgagcgg ttgcgtctca tttacgtgac gaattctcgg
     2701 tcggtggttg aagtcagccg aatcgttggc ggtaccgggc gtcggcgtcg ctgcggacgg
     2761 tccgcgcaaa gtcaagggat tcaccgcgca gatagccagc cacaccgagc gccgtggcga
     2821 tagagccggg gatccaggcg gcccatgcag cctcgttacc ggcgaaaccg gcgagccacg
     2881 gcgcgaggaa caacgcaaaa ccgacgacgg ccatggtgaa gtagtcacgt gtggggtcgg
     2941 ccgcgagcat tgaccacatc gcgatgaccg cggtgaagcc acccaacccg accgtgacgg
     3001 cgacggcggc cggggaggat tgcgcgaccg tggctcccag caccacggtg ccgagcccaa
     3061 tgacaagtgc tgctctactg atccaatcgg ccacggcgct gggtctcgcc tggctgctgg
     3121 tgttctcgtt gatgccatat atggtgggcg gcctgctgct tagccatccg atcgcgccga
     3181 gggccatggc gaggaatccg accacccatg ccgtccatgc ggctgctgga tctgggacga
     3241 atcctgcgcc gagccagggc agcaggaaca gtacaaaacc ggccacgaac agcccccaaa
     3301 gactgggggt ctgattacgt gccagcagtg acaggagcgc gaagagggct ataaaggccc
     3361 cgaaccccaa tgtgagacct tgcccagctg gtgttgaagt cagcgtgatc gacgacacga
     3421 cggcgccgat gcccgctatc agtgcggccg agccgatcca gtatgtcggt gtactcctca
     3481 ctaacccgct gaggctcgat cctgcgtcgg cgcgggtgcg gttagtcagt tcagttgggg
     3541 cagttgattt ctgcgtctga agtcttgggt tttgcattgg tggttcctat gaattctatt
     3601 ggttgaatag ggatttgagc gtcgctgccg cagtcgattg gggcgcgact tcgagtgctc
     3661 aggcgtcctg catctcaagt tgagaccatg gggctggtgg cactgcgcct ccgtcagatt
     3721 cccatcgctc aagtgcgatg ggtgagagca ggcgatcgtc cgcgcggcga gtgatcccga
     3781 gtagttggtc gggtgtcgac gcgtcgttga cgctggccgc agctgtcacc acgttgtagg
     3841 catgcgtggg ctcggtgtac gggggcacca ccagcaggtc caacctcccg aaccgcggac
     3901 cgaacacaga tacgacgttc ggccactctt ggtgggcact gacgattacc tgttcgccct
     3961 ggtgtttgat gctcagtggt gccggggacc agtcactttc gtggtagagc acgctgtcga
     4021 ttgagccgaa ccgtaaggag agcgctgcca gaagtgaggg aatttcgctg gccagttctg
     4081 tcgatcgggg ccaccaggcg ccttggacaa acccgcacga ccggtgtgcg ggcttcaacc
     4141 gcattcgcag ggcgcctagg gctggccggc gcactgatgc gtcggggatg ttctcgttga
     4201 ttgttaacat gtgctgcctt cccttgttgt gggatgtgtg ttcacggcga gttgtgttcg
     4261 ggtgccaggg ttgtcgagcg caggggggct cgccgtcgtt gcgctcgatg tccccaacag
     4321 ccaccacggt gaacgaaggt ggatatcttc catggtggta tgaatttgcg tgactggctt
     4381 gccgccgttc gacaactgca gggccggtct ttgtcgcggt agaacagggc aggatcggtc
     4441 ggtcggtcat cgccgcactc gatgtcgcgc gggatctttg gtatccggag attcttgccg
     4501 aaggcgccgt cgcggcccac gcattgtcag cggtcgtcac gcccgtatgc gacggcggcg
     4561 atgagccaca acacggcgat gatgaggaat accgccccga tcgggtcgat gtcaaacata
     4621 tgggaactgc ctcggtttga cgcgggccgt gcccgtgccg gatgcggaaa ccggcgcggg
     4681 agtattcgcg ggtacaggtg gtcgcgccgc aggaccgagg cgctggcctg actgctcgcc
     4741 gacgtggtca ccgatgccga acctgtcatc actgccatcg ttggacggcg aggcttgcag
     4801 cgcactcgaa cagagttcgg ccgcgctgcg cgcgggatct tcgccggtga gttcggtgat
     4861 gacggcacag agggttctgt tttgggcgag tagctcagct tcgcgggctt gaagttcgcg
     4921 aattcggtgg tgggccttgt tgagttcgtc gttcagcgtg gcgaacgtgg gagctgttgg
     4981 agacatggga cttcctctta accgtctgtg ggtgttgtct ttcgatcggt ttgcgatctc
     5041 actgcggtgc gggctggctc aggtcagcga tgtgaaggcc gtagggcact gctttgagga
     5101 gcgtgaccgc caggggagtg ccattgggca gtctgtaggt gcgccgttca ccagcgcgtg
     5161 cccctgtgat cgcggcgccc aacggggact cgatggaata gacgggcatg tcggcgaatt
     5221 cggcgccgtg tacgcccagc aggaaagtct cggtgtcccc ggtggcgtcg cggcggatcg
     5281 tgacgaccat gccgggttcg gctattccgt cgttgggtgg gtcctcaccg acgacggcgt
     5341 tgatcagtag gtcatgaatc tgttgcattc gtgtctccca cgcgcgtcgg acggcggtgg
     5401 cgttttcgtc ggtgccctca tcggcgattc cggctgagaa caggaaacgc agggtgtcca
     5461 gttcgcggtg tagccgctcg tgggcttgcg gcgagatcca gacacgtgtc gtggtggcca
     5521 tgtcagttcc tttcgttgcg cgagttcgcc agcgcagtgc cgtatttgac gactcgctaa
     5581 cgcctcggcg tgggtggtgg ttgggcgcat cgttcgcttc gccaataggg cgtcgggcac
     5641 gcaccggtga gcctggtagc ggaggcacgt tgatgttcgt cgcgccgggc tccgtcaggt
     5701 tgcggccgga acgcgacgcg ctgtcgttgt gcgcttggcg gcgggtcgag gacgggagct
     5761 gttgcgtcgc ggtgggacag actgtgaccg tgggggtttc ggcgtgtgca ttccgtaggg
     5821 gaccgccttg aggagcgtgg cgggcaggtt ggccccgctg gggatggagt aggtgcgctg
     5881 ttcgccaacg tgtgcgccgg cgatcgcgcg cccgagcggt gattgcatcg agtacacgtc
     5941 gatgtcggcg tcttcgacac cacgcacgcc gagcagaaac gtttcgatct cgccggtgtc
     6001 gtcgtagcgc acggtcagca ccattcccgg ttcggcgatg ccgtcatcgg gcggatcctc
     6061 accgacgacg gcgttgccca acagctcctg aatctggtgg atgcgtgctt gtcgcgccaa
     6121 gtgggcggcc atgagattgt cgtcatagtc catgaagtcc tcagggactt cgatgccgcg
     6181 ctgtgaacgc agggcggcca gttcggtctt caggcgggtg tgggcctcgc gtgtcatcca
     6241 aagacgttcg gtgctagtca tttcgggggt gtccttttcg ttgaggtagg ggttgtcgtc
     6301 aggggcgggc cggtggcgga agcgccgccc ctgacgactc gggcacccgt gacgagggtg
     6361 gatcacgggc gttgtggacc gcaggtttgg gtgtgttagc gcccggttcg gcgtagcggg
     6421 tcgaagtcgc gcagggcttc gggttgcttg ccggtggtga tctgttcggc cagaagccgg
     6481 ccgctcaccg ggccgtgcgc gagcccccac ataccgtggc cgcccgcgac gtagacccct
     6541 cgtgacatct ccccgatcag ggggcggccg tcgggggtga ccgggcgcgg accaacccag
     6601 atgtcgctgc gttcggccca gcgcaccccg tccagcagtg ggctggcgga ggcgacgatg
     6661 gcgcccaccc gtgcgggtac cgccggttcg tgcgggtcgc gaaactccat ggttcccgac
     6721 acgcgcaatg cgccgtggta gggcgtgcac gccacccgca cgtcgggcag gtagatgggc
     6781 ccggggatgg ggcggtccac cggcacggtg aacgagtagc cgcgaccggc ctgcaccggc
     6841 acgcgcaccc ggctacccgc cagccgggat agccatgcgc cggtggcgat caccgcggtg
     6901 tcggcggtca acggcatgcc gctgcgcggg tagaccgtga tgccgctgcc ggagcggaac
     6961 acgctgcgca cttctgccgt gcgcatcgtt gcgccgcggt cggcgaccgc ttggcccagc
     7021 gcgtgcacga atcggccggg gtcgacgaag cgctgaccgt tgatgctcag cgcggcggtg
     7081 atcgccggtg atgccagggg cacttgttcg cgcagtgcct caccggatag tgcggtgaca
     7141 gacattgtct gcccggcgtt ttcgagcgcg cgaagctcgc gcatcatgcg ttcggcttgt
     7201 tcggtgctgc ggaacaacgc ggtgatcggg gcgtcggtga ccggtgcgtc gacaccgttg
     7261 gcgacgagca cgtcgaaggc ttcgatggct tcctcgttga gcggcacgtt ggcgcgtacc
     7321 gcccgtttcc acgacgaacg gcgacagttg gcgacgaatt gcgtcaggaa gaccccgagt
     7381 gcggagtcgg cggtcatggg gatgtgcagc ggggcggccg ggtcgagcag cgcgcgcagc
     7441 ccgtagcgca gcacgcctgg tgagttcagc ggaagggtta acgccggtga gatccagccg
     7501 gcgttgcccc acgacgcgcc ggcggccacg ccggtgcggt ccaccacggt gacatcgact
     7561 ccgcgctcct gcaggaacca cgccgtcgac aagccgacga tgcccgcacc gaccacgatc
     7621 gccgaccggg gcccgccgtc aatgcgctcg ccgccgccca tcacaaacct ccacatcttg
     7681 atctttcgtt gctatctcca tggtggcctg caccacgcgg gaaccgtttg ctggattccg
     7741 acaagcagcc gatgccagat tgtggcgatc cgacaacgcg acctcttgag cgcgccggtc
     7801 ccctggaagc aatgagtgca cgcgagtgaa aagcacgaaa aacgacgcag gtggcttttt
     7861 attttttctg acgtatggtc gggggcaggt ggttgccact tcgattaagg agcgacaatg
     7921 gctgacaaat ttccgggcaa aagtcttcga aagcccgcac tgtctatcaa ggaacgacga
     7981 gctgccaagc gcgccaaggc cgtagagtcg acaccgattg tgcgcaaacg caagcgctga
     8041 ccgtacaggc ggttttgtcc gccggtatat caagaatgcg acacggatgc cgataaccgg
     8101 ttgttgccag tgtgggcacg ccctttttca tgcgtgtcgg ctaccggggg gaatcagttc
     8161 gatcgtcacg ctggacacga atgtgcgaat cgagtcgaag ttatgcggcc accacgacgg
     8221 ccacggcgag ttgctcgtcg gtcgagcacc ggtacaccgc gcagcaattt gccgggcgcc
     8281 gctcttgggt ccggccggat cgatgtgtcg taggcatggc ggggtgatgc aatgtatgcc
     8341 cacaagccgg ctggaccggg cgggctaagc agtactggtg gttaccgagc gcaatggggc
     8401 gcaccccccg cgttgatgcc ccggcggcgt cacatttcgc cgtcggaccc accgtggtgg
     8461 tctgtcctcg atgcaggacg ggcctcctgc gccgcgcgca tcccgatcgc aagcagttcc
     8521 tcggcggtcg acacgtcgtc ggggtcggcc gccgtcatca cggcggtgtg cgcagcgacc
     8581 gggtcggtgt cggggggaac caccagcagc acgagcgttc cgaacttctt gccgctgact
     8641 gtgagtgtgt tcggcgatgt gttgctgggc tcgaggatca cgctgtggcc gcgaaactcc
     8701 atccgcaatg atgctgaggc ccagctgttc tcgtcgtaga taacccgttc gatggtcccc
     8761 gaccccgacg acaacgccga cagtaggaac cgaagctcga cgtacagttg gtcggttagc
     8821 ggccaccagc cgccctgcac aacgcctgca gaacggtgcg cgggcttcaa ccgtaatcgg
     8881 ggatgcgtga tcgtccatcg gcgagcttgg gagatgtcga aggacaacgg tggtgcggac
     8941 atgagtgggc tctcccgtac ttgtgaatga atgggctaca tgaggattgg cgacggcttt
     9001 agggctggct gggtttgtgc ccgtcgttgt cgaccaagtt gtctaggtcg tgttgccatc
     9061 gtgtgaagcg gatgcggtca caaacggccc gggtgagggt gaacagggtc gctgcgatgg
     9121 cggccacgca tatccagatc acgagcgcgc ccattgccgc ttccgcggcg gcacggctgg
     9181 tcggggctgg tgcggggacc tgcgatccgt tgtcgtcgac ccagatctca agggtgtcac
     9241 cagccttggc tgtcgattgc gccttgaccg tgccggtgtg ttcggcgccg gcggcagtcc
     9301 atcgggccga cacggtggtg gtacctgttc gcaagatttg ttgggaggca gggacgtcgg
     9361 tgaccgtcgc ggccacggta tggcggatgt gggcctgctc ggcgtagata tggcggctcg
     9421 agtcatagac tgcggtcccc acggcggcgg cgatcggggc ggcaagcagc gacaccacga
     9481 cagccagcac taggaccagt gcctcgatcc gatcgatggt gcggaccagt ggatcgcggc
     9541 cggccaggcg cagcagaacg ggccaccggg gtagacgcac ggtgaaggtg tccatcgcgg
     9601 cttcgtcagc acggcggtga gccgtcccgc gttgatcgtg gggaccgtgg gatttcatcg
     9661 acatccccgc tcagcgagtc ggcgtcgccc ggtggcgccc cgattgctaa cggtgtgatc
     9721 ggcaggtatg gcgtcgagtc gcttgctggc gatcatcacc cacctccaca gctcgaccat
     9781 tggttatctc catggtggtg tggatggcgg tcgcagggtt tgccccagtt agacaacgtc
     9841 gccgtggcgc tttgtcgtgt ttcgacagcg catcgtcagt cggtgtcggt ggccgcgagt
     9901 tcgatcatca tggccagccg ctttttggcg tcgtcgagtt gcagattggt gatctcggcc
     9961 atcttgcgca gccggtagcg cacggtgttc tcgtgtacgc ccagccggtt gcccgcttcg
    10021 gtcgggtcgc cctgggcctc cagccaggcc cgcagcgtcg gcacgtaatc ggttgtgtgg
    10081 tcgcggtcgt ggcggcgcag ttcggccacc ggtccccggt ctggggatcg acctgagcgc
    10141 gccgcggtgc gcagccgctg cagcaggatg tcgtcccacg attcgtcgta ggccggaggc
    10201 gtggcaccgg taggccatat ctcgtgcagc gccagacact cgtcggcctc gtggcgggcc
    10261 gcgacgaggt cggccacctc ggcgggcccg ctgatgccgg ccaataccgc catccgctcg
    10321 ggaagtgctg cccgcagacc ggtcacccag cgtcgcgccg tgtccacctg ctcgccgggc
    10381 agcagggtgt agacggtgtt gccggccagc gcgctgcgcc cgggccgtga ccagccgaac
    10441 ccggtggtgg cgcgctcgaa cgccaacagc agcgcggcgt ctcgctcggc cccgatgaac
    10501 gcctgcaccg cgatcacccg caacagggtg tgcggaaggc ccagtcggct ggccgccgtg
    10561 gcggcatcgg cggtgccctc cagcaaccgg atcaccagtt cggattcgac ctggcgctcc
    10621 agatccgcgc tggcacgcga gcgcaacagg tgcagagcaa ccgtgcgggc gccgtcggcc
    10681 aacgcggtga gcgcggcccc cttcagcggc gcccgacact ccacccacac cgatcccatg
    10741 agttcgcgac ccgagcgcac cgccaccacc atgcgcccgg tcatcccgtg atcagggtcg
    10801 cgggtgacga ataccggcgc gtcggaggcc gccagatggg tgtacacgcc gcgggcgtcg
    10861 aagaatgcgc gcaactgctc gggtgcctgc cgttcgacga tggtcgccac ccgcgccggg
    10921 tcggcgtctt gctgccggcg ggaataggcc agcacgcggg agagccggtc ctcgatgatg
    10981 accgcgccgc cgctggcctc cgccaaactg tcggccagtg cgaacagatc cgttgggcca
    11041 cgcccggatt cggtttcgcg gccttccaag actagtccgt agatgaccgc ggccaactcg
    11101 ctccaggtca tcgccgggtc gaccaccatc accgcgaccc cctcggccgc accggagaat
    11161 gcggtgtcgt catcgtcgcc ttcgcggatc agcacgacaa cggcccgcgc tgatgcggcc
    11221 cagcgcaccg cctctttgac tgagcgcgca ccgatgccca gcagcacgtc gccgacgacg
    11281 gtgcgatcct cggtgcgctc atgcatgacg acgctgcgca actcggtcga ccgcggtatc
    11341 gggcagaacc gcaaccggac gccgtagctt cccagcacgt tcaccaggcg gtctaacgtg
    11401 accacgagcc gctccccagg ccgaaccgat caaccagcct aaccagcagc ccaccgagcg
    11461 gtcggtgaat cacagctccg tcgaatcggt gtggtcgccg ggaccgtcgt tgtcggtcat
    11521 gaagtcgttc acgatggcag cctcgggggc tgttcgcctc atagcccagg cagatcgtcg
    11581 tcgtaggccc tttcgccgta gcgctgaaca gttttggcga cggtgagcag gacgacgcag
    11641 gcgtcgacgg cgtgcaggcc gtggcgggtg gaggggatga cgaggtgatc gcccattggg
    11701 gctgttccgg ttctcagtga cccgatggtg gggtcagttg acggtgaagg tcggcgacgt
    11761 agccgtcgta ggcggcggcg accgtgtcga ggtcgacctg ctcgggcagg tcgtcggcgt
    11821 tttgacgggc gatcaggtag gccgcggtcg cctcgacgac acgctgctcc tcggcagcga
    11881 tctgcgcaac cacggcgggg tcggcgtgca tgcgcacgac aacggtgtcc tcgttctgct
    11941 gaaaccggac gagatagtcg tgatcgccga gcgcctcgag ggccggctga tgaacggtca
    12001 tggtgttctc cttcgtgggt ggttgcgggg atcgtcatcc cgccaggagg gcggcgccca
    12061 gggcgaccaa ggcggcgagc ttgagtgcct ccaacccgat gtaggcgtgg tgtgcgcgtg
    12121 aacgcggtgc gtcgtggccg gccagcacgc gggccgagcg ccgattgagc ctgggccgca
    12181 ccaccagcag ctgcacggc
//
LOCUS       DASRJA010000516         3138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3707108,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000516 DASRJA010000000
VERSION     DASRJA010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3707108"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..758
                     /locus_tag="VFC01_22525"
     CDS             273..758
                     /locus_tag="VFC01_22525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82428.1"
                     /translation="MLKRLLIIGALVGFAAAPAHAAAVVGGAGPRVGFSIDPDQIVFG
                     GQLIIGEVAPQLTFDPSLEFGFGDHYTVIAANFDLHYHFAIQNSAWRPYAGGGIGLNF
                     IEFDSNDPFVDNSNTEVGGNVVIGAGVPTQGGNRFFGEMKFGLGDIPTLKMLVGWNFK
                     I"
     gene            1295..>3138
                     /locus_tag="VFC01_22530"
     CDS             1295..>3138
                     /locus_tag="VFC01_22530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82429.1"
                     /translation="MITHRRPAGIPVWLGMVALVGALVAPHGFARADTQSCTSEMGGV
                     EIDGNLVADAPGKYDWFGPAGQGFLQTGASCGQPIQQAIIGNLAIKCISPMVRDDCNA
                     GGSKDASVFGSTADKNNSCLVTGLQPWQCKTGDVPQKNDLTEAYAAVFQDQEPTSPTF
                     GDHLLFVAVGHRSTDGDNHLDFEFNQAGVTTCINGVIVGGPGTELTGGRTLGDFIVSV
                     DFSGGGNNGQVSLRTWEANAGAPCGYAYSNPIPDQLGPLGFACTNTNPLDDKADVPVT
                     APCQAWQPSSQCQLGANYETRQFIEVIVNLSDAGVFDDLVCGANSTLMYKSRSAPSFT
                     AELKDFIRTEFQILPPPPCAIVGPDSVCPGATAQFCGPDGFIYSWTATGATIISGDPT
                     AQCVTVQAGSTCGSYTVELTVSDATSCSGTCEKTVNVTDTSNPVLVGVPTGGDLGCNP
                     TPPSCATVTATDDCDASPTVTCVPGTITGTCNKSQTFTYTATDDCGNSAVSTATYTWK
                     EDTTNPVLVGVPTGGDLGCNPTPPTCGNVTATDNCDGTRPVTCTPGTITGECLKSQTF
                     TYSAVDLCGNSASSSATYTWKEDTTNPVLVGVPAGGDLGCNPTLPTCA"
BASE COUNT          556 a         1065 c         1000 g          517 t
ORIGIN      
        1 gcatcgaggc ctcgccggcc cacgccgccc ggccgcccac cgcccccagg taacgatcga
       61 cgacggcctg ggcctcgggg gtgatctcgg ccgacgaggt ccggggtgaa gcgcctgcca
      121 tcagcaccag gcacgcgaac agacggcgcg cgggaagcga catcttcatg acattcctcc
      181 gacggatggt tgatgcgagc agcgacgcat tataagctgc cgcctcgtgc tccccgtgcc
      241 ttgatcatgt tctccatacg gaggtgggac agatgctcaa gcgcttgctc atcatcggtg
      301 ccctggtcgg attcgcggcg gcaccggctc acgccgcggc cgtcgtaggc ggagccggcc
      361 cgcgagtggg tttcagcatc gatcccgatc aaatcgtctt cgggggacag ctcatcatcg
      421 gcgaagtcgc cccgcagctc acgttcgacc ccagtctcga gttcggcttc ggcgatcact
      481 atacggtgat cgctgcgaac ttcgacctgc actaccactt cgccattcag aactcggcgt
      541 ggcgacccta tgccggcggt ggaatcggac tcaacttcat cgagttcgac tccaacgatc
      601 cgttcgtcga caactcgaac accgaggtcg gcggaaacgt cgtgatcggc gccggggttc
      661 ccactcaggg gggcaaccgc ttctttggcg agatgaagtt cgggctcggt gacattccca
      721 ccttgaagat gctggtgggc tggaacttca agatctagcg tcgcaggacc aacgccccgc
      781 gagtcgggac cggcgtccta gcccgttagc ggcgacctcc gccgcccgtg gtggtgcacg
      841 ctcatgatcg ggtgccgcac aagacgttac agcgcagctt gacatctgct tcgcgtcgcc
      901 gcccttcaca acctccggac gatgcgcacg ttgcctgaaa tagttcagtg atttgtcttg
      961 ccattgcatc ttgcaagggc tacgactccc gctctcgact tggccgcaag tccacggacc
     1021 cggtccacac aattcgccga gtcgttttgc ggaggcacca aactccgagt cctcacgtcg
     1081 cccttgcgca gaggtgccct gcagtggcga cagtcgcatg gaggtttgcc cgtcgttctg
     1141 ccgcctacaa ggcgagaccg cgtacgcccg tcgtgaacca accgagatcc gcatcctgcg
     1201 cgcacgccgt cgagggttcg ccctcagttt ctcgaatgtc cacgcagccc aattgacctc
     1261 accctgtcac tgaattcttc cgggagaaac tgccatgatc acgcatcgta gaccggccgg
     1321 catccccgtt tggttgggga tggtggcact ggtcggcgcg ttggtcgccc cgcatggctt
     1381 cgcgcgggcg gacacacaat cgtgtacgag tgaaatgggc ggcgtcgaga tcgacggcaa
     1441 cctggtcgcc gacgcccccg ggaagtacga ctggttcggg cccgcgggcc aagggttcct
     1501 gcagaccggc gcgtcgtgcg ggcagccgat ccagcaggcg atcatcggaa atctggccat
     1561 caagtgcatc agcccgatgg tgcgcgacga ctgcaacgcc ggtggcagca aggacgcctc
     1621 ggtgttcggc agcaccgcgg acaagaacaa ctcctgcctg gtgaccggtc tgcagccgtg
     1681 gcagtgcaag accggcgacg tgccgcagaa gaacgacctg accgaggcct acgctgcagt
     1741 gttccaggac caggagccga cctcgcccac cttcggcgac cacctgctgt tcgtcgcggt
     1801 ggggcaccgc tcgaccgacg gcgacaatca cctggacttc gagttcaacc aggccggcgt
     1861 gacgacctgc atcaacggcg tgatcgtcgg cgggccgggg acggagctga ccggcggccg
     1921 cacgttgggt gacttcatcg tcagcgtcga cttcagcggc ggcggcaaca acggccaggt
     1981 gtcgctgcgc acctgggagg ccaacgccgg cgcgccttgt ggttacgcat acagcaaccc
     2041 gatcccggac cagctcgggc cgctcggctt tgcctgcacc aacaccaatc ccctcgacga
     2101 caaggcggat gtgccggtga cggcgccgtg ccaggcgtgg cagccgtcgt cgcagtgcca
     2161 gctgggcgcc aactatgaga cgcgccagtt catcgaggtg atcgtcaacc tgtcggatgc
     2221 cggcgtgttc gatgatctgg tgtgcggcgc caactcgacg ctgatgtaca agtcgcgctc
     2281 ggcgccgtcg ttcacggccg agctcaagga ctttatccgg accgagttcc agatcctgcc
     2341 cccgccgccg tgcgcgatcg tgggccccga ttcggtgtgc ccgggcgcca ccgcgcagtt
     2401 ctgcgggcca gacggcttca tctactcgtg gaccgccacc ggcgccacca tcatcagcgg
     2461 tgatccgacc gcgcagtgcg tgacggtgca ggccggctcc acctgcggct cgtatacggt
     2521 cgagctgacg gtgagcgacg cgaccagctg ctcgggcacc tgcgagaaga cggtgaacgt
     2581 gacggacacg tccaacccgg tgctggtggg cgtgccgacg ggcggcgatc tgggctgcaa
     2641 cccgacgccg ccgagctgtg caacggtgac cgctaccgac gactgtgatg ccagcccgac
     2701 ggtgacctgc gtgcccggca ccatcaccgg cacgtgcaac aagagccaga ccttcaccta
     2761 cacggccacc gacgactgcg gcaacagcgc ggtctcgacg gcgacctaca cctggaagga
     2821 agacaccacc aacccggtgc tggtgggggt gccgacgggc ggcgacctgg gctgcaaccc
     2881 aacgccgccg acgtgcggca atgtgacggc gaccgacaac tgtgacggca cgcggccggt
     2941 gacctgcact ccgggcacga tcacgggcga gtgcctcaag agccagacct tcacctactc
     3001 ggcggtggac ctgtgcggca acagcgcctc gtcgagcgcc acctacacct ggaaggaaga
     3061 cacgaccaac ccggtgctgg tgggcgtacc ggccggtggc gatctgggct gtaacccgac
     3121 gctgccgacc tgcgccaa
//
LOCUS       DASRJA010000517         9351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2445881,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000517 DASRJA010000000
VERSION     DASRJA010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9351
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2445881"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFC01_22535"
     CDS             complement(<1..459)
                     /locus_tag="VFC01_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccE"
                     /protein_id="HZN82430.1"
                     /translation="MTVRIALASLFIVPAAMAYRWPSTADRWLLGGAVVAVLVLFAGW
                     RGLFFTTMVWRRLAIWSRRYRTDGLHHSPVDYSTVVLRIDPSEPVALPLRLIAGYLDR
                     YGIRCDKIRVTSRDLDGLRTTWISLTLRAVDNLTALRARSARIPLRETTDV"
     gene            complement(456..995)
                     /locus_tag="VFC01_22540"
                     /pseudo
     CDS             complement(456..995)
                     /locus_tag="VFC01_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            complement(976..1504)
                     /locus_tag="VFC01_22545"
                     /pseudo
     CDS             complement(976..1504)
                     /locus_tag="VFC01_22545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891330.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion AAA-ATPase EccA"
     gene            1639..2559
                     /locus_tag="VFC01_22550"
     CDS             1639..2559
                     /locus_tag="VFC01_22550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN82431.1"
                     /translation="MTIRLGLQIPSFSYGAPVAELFPAVKAQAQEAESAGFDTVLVMD
                     HFYQLPTLGKPDEPMLEAYTTLGALASATERVQLSTLVTGNTYRNPTLLAKEITTLDV
                     VSGGRAILGLGAGWFELEHQQLGFEFGSFTERFEKLEEALQIVIPMTHGERPSFSGKW
                     YHTENAINEPRYRDHIPVMLGGSGEKKTFRLAAQYADHLNIIAPMDELPGKLVALEQR
                     CAEANRDPATLETSCLLTVVLDGYESHGAIDEARGGRAVSGSPQQVAEEIKRRVLDVG
                     VDGVIVNLPTHRYTPGVITKVGEVLRSISS"
     gene            complement(2549..3442)
                     /locus_tag="VFC01_22555"
     CDS             complement(2549..3442)
                     /locus_tag="VFC01_22555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN82432.1"
                     /translation="MRTDNDSWDIKTSVGSTALFVAAARALEAQKPDPAAVDPYAEVF
                     CRAVGGEWADLLDGGAPDHPLKTEFGEHFVNFQGARTRYFDTYFQAAAAAGVRQIVLL
                     AAGLDSRGYRLTWPDGTVVYELDQPRVLEFKREVLAAHGDTPTTERREVAVDLRNDWP
                     QALRDNGFDPSKPSAFIAEGLLIYLPADAQHQLFAGIDSLAAPGSHLAVEEAVPLDAD
                     VFEAKREEERAAGDEDTFFTLVYNEQHAPAHEWFGHRGWRSVATPLPSQLRSLDRPVP
                     DPESEAGAMTGNISLVSAIKN"
     gene            3491..4084
                     /locus_tag="VFC01_22560"
     CDS             3491..4084
                     /locus_tag="VFC01_22560"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN82433.1"
                     /translation="MAEPWNINIHYDGKLDRCVPPEVGSVLDVGCGDGFLAARLSQRI
                     PTVVAVDIDQPVLARARQRFPSAPVKWRCGDILDIADDLGAFDAVVSNATLHHLPDTR
                     VALRRLGSLVTPGGTLALVTFARPGWRHLPWAMATLVIRGVAIRLRGKWEHSAPTVWP
                     PRYTVAQLRRDVRAELPGARVSLLPLGRVFIQWQATG"
     gene            complement(4085..4984)
                     /locus_tag="VFC01_22565"
     CDS             complement(4085..4984)
                     /locus_tag="VFC01_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZN82434.1"
                     /translation="MTISEPVQHVERAVSEPAAALPTTSRRRGANFDDGLMGVALLAG
                     PANVIMQLARPGVGYGVMESRVESGRVDLHPIKRARTTFTYLAVATKGSPEQKAAFRR
                     AVNRAHAQVYSTDESPVKYHGFDKDLQLWVGACLYKGAVDVYRLFIGEMDEETAERHY
                     REGVPLGTTLQVPEDMWPKDRAAFDRYWRESVEKVHIDDAVREYLWPIAAARVRGVKL
                     PGPIQRALDNFSLLITTGFLPQRFRDEMRLPWDAVRQRRFDRLMRALRTVNHLLPRFI
                     RQFPFNVLLKDLDWRIRTGRPLV"
     gene            5059..5685
                     /locus_tag="VFC01_22570"
     CDS             5059..5685
                     /locus_tag="VFC01_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN82435.1"
                     /translation="MDPMAHVRPYRGIEAADRVAERRRRFLEAGLDILGSDPDLSELT
                     VRGICREAGLALRYFYESFTDKDDLVAAVFDWVIADIAATTQAAVAAAPVKEQTRAGI
                     ANIVRSVAADTRVGHLLFSPQLANSVVLRKRAESFALMAMLTFQHAATLGAKDTDLVK
                     AASHFVVGGVMQMIAAWLAGDVRMDTDQLVDQLAAMIDALAVPELYRG"
     gene            complement(5682..6263)
                     /locus_tag="VFC01_22575"
     CDS             complement(5682..6263)
                     /locus_tag="VFC01_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN82436.1"
                     /translation="MIKPDNPNSEFELGGINHVALVCSDMARTVDFYSNVLGMPLIKS
                     LDLPGGMGQHFFFDAGNGDCVAFFWFADAPDRVPCVSSPEAIPGIGDIVSAVSTMNHL
                     AFHVPAEKFDEYRQRLKDKGVRVGPVLNHDESEAQVSATVHPGVYVRSFYFHDPDGIT
                     LEFACWTKEFTERDTKTAPKTGADRRPRVPAGR"
     gene            6339..6971
                     /locus_tag="VFC01_22580"
     CDS             6339..6971
                     /locus_tag="VFC01_22580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN82437.1"
                     /translation="MTVSPREQLPTLRGRQTQAAIDAAARAVIARKGILATTISDIAA
                     EADRSTASFYNYYDSKEAMVREWAVRFRDEARERSRAAAEPGLTNWERSYQAAAAHWN
                     TYRNRLAEMISVSQLAMVSDDFAQYWSDICSLPIGLITQIVKHAQRQGFCAADDDPQL
                     IAEAMVSMLNQFCYTRLAGNMAAAVDDDACITTLANIFYRTIYPKEAGLL"
     gene            6968..8110
                     /locus_tag="VFC01_22585"
     CDS             6968..8110
                     /locus_tag="VFC01_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN82438.1"
                     /translation="MTKTPASSGTPGVTREFVGTDSPTARRAGSGGHPCQGLYHRGVG
                     RKPKVAMIATHYQIDFSEHYLADYMATRGIGFLGWNTRYRGYESSFLLDHALVDIGIG
                     VRWLREVQGVETIVLLGNSGGGSLMAAYQAQAVDPHVTPLEGMRPAAGLTDLPPADGY
                     IASAAHPGRPDVLTAWMDASVVDENDAIASDPDLDLFNEHNGPPYPKEFLERYRSAQV
                     ARNHAITDWVETELKRVHAAGFSDRPFTVLRTWADPRMVDPTIEPTKRPANMCYVGVP
                     VRANRSAHGIAAACTLRNWLGMWSLRHAQTRAEPHLSRIECPALVINAEQDTGVYPSD
                     AQRIYDALASTDKSACSIDTDHYFTTPGARSEKADTIAKWIAKHWH"
     gene            8107..9045
                     /locus_tag="VFC01_22590"
     CDS             8107..9045
                     /locus_tag="VFC01_22590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HZN82439.1"
                     /translation="MRVLAHFTPGDKVTEFLAPESDWLDIRYCAEDDDDTFYHELPEA
                     EVIWHVLRPISGHDLEKAQRCKLVHKMGAGVNTIDVAAATRLGIAVGNMPGANAASVA
                     EGTVLLMLAALRRLPALDRATREGRGWPSDPSLGETVRDIGGCTVGLVGYGNIAKTVE
                     TMVTAMGARVLHTSTRDDGHPGWRPLPELLTESDIVSLHLPLTDKTAGMLDREALATM
                     KTDAVLVNTSRGGVVDEDALVDALRTGGVGAAGLDVFAVEPISTDNPLLGLDNVVLTP
                     HVTWYTVDTMRRYLEHAVDNCRRIRDGQELANVVNE"
     gene            9098..>9351
                     /locus_tag="VFC01_22595"
     CDS             9098..>9351
                     /locus_tag="VFC01_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN82440.1"
                     /translation="MPIAITPEHNDLADSVRSLVARVAPSDVLHEALETPIPNPPTYW
                     KAAAEQGLQGVHLAESVGGQGFGILELAIVLAEFGYGAVPG"
BASE COUNT         1704 a         3084 c         3023 g         1540 t
ORIGIN      
        1 cacatcggtg gtctcgcgca acggaatccg cgccgatctt gcgcgcaacg cggtgaggtt
       61 gtcgactgcc cgcagggtca agctgatcca ggtcgttcgc agaccgtcga gatcccggct
      121 ggtaacccgg attttgtcgc agcggatccc gtaccggtcg aggtatccgg cgatcagccg
      181 cagcggcagc gcgacgggct ccgacgggtc gatacgcaac acaactgtgg agtagtccac
      241 gggggaatga tgaagaccat cggtgcgata gcggcggctc caaatcgcca gtcggcgcca
      301 aaccatcgtg gtgaagaaca acccccgcca ccccgcgaac aacacgagaa ccgcgacgac
      361 ggcgccgccg agcagccagc ggtcggccgt tgacggccac cgatacgcca tcgcagccgg
      421 cacgatgaac aatgacgcca gcgcgatgcg gaccgtcatg aggtggtgtc ctttcgtcgg
      481 tgcgcgccga tcgcgacggc gacgagcgtc gccaacgtca gcgctgcagt gccgatgaac
      541 gcgacattgc gcggcgtggt gtcggcgggc ggcggggtcg gaggcgccgc gacctgcctg
      601 acggccgcgg ggtctggacc ggacgcctcg ggcacatccc aggtgagcgc cgcgaccgcg
      661 tccatgctcc ctgcgccgac cagattcgac ggcgaacgcg ccgcgacgcg cgcggtcgcg
      721 ccgatgcgat gcattacctg agccgccgtc aattcgggaa acctgctgcg caccaacgcc
      781 acaacgccgg acacgtacgc ggcggcgtag ctggtgccgc tgatcgggaa caactcatgt
      841 tgatcattgg gtagcccgtt cgccagaccg ccgccggggc cgttgctcac cgagacgatg
      901 ttctctcccg gggcggcaat gcccagccag gggccggcca tggtgaactc cgacggctgg
      961 ccgctcgcgg tcaccgagcc gacggacagc acatgaccac cgctcggctc gatggcagat
     1021 cgccacccgt agccacgtgg cttgcagcca atcgggccga cggttcagca gttcaccgac
     1081 cagtcgatcg gcgccagcgt ccttggggct atcggaaagg gttgccgcat aggccaattg
     1141 gaagtcgtcg gggtcggtcg cgcggaactg gagatagaga ccggtgtcgt aggtgaatcc
     1201 cagcgcccgt ggcgcaaggc cgatctgccg ttggagtacg ccggcggagg gcgatgtccg
     1261 ccagatggcc tcgacgacgt ggatatccac ttcgccggcc gcggccaggc cggtccacgc
     1321 atcgcactgc tcatgcgcga cgcgggtcaa gccggcgaac ccagttcggg ctgcggtgat
     1381 gtcgacgggg cgctgccgat catgaatcga caggccaagg gcgcgacaac aggtggcgaa
     1441 ccggccaatg acgtcgcgat cgacccgggc aagggcatga ggccgccaga gtgtcactcc
     1501 ccacgccgtc tccttattta gccatgcctt agctaactcg ggtgaagtta gcattgcata
     1561 agctaagtgt cgagacgtcc gtctgacgat cagcccgcgg ccggaataaa tcaagcggag
     1621 aggcggtcac gatggtgcgt gactattcga cttggattac agattcccag tttctcctat
     1681 ggagcgcccg tagccgaact gtttcccgcg gtgaaggcgc aggcccagga agccgaatcc
     1741 gccggattcg acaccgtgtt ggtgatggat cacttctatc agctgccgac gctggggaaa
     1801 ccggatgaac cgatgttgga ggcctacacg acgctgggtg cgctggcctc agcgaccgag
     1861 cgggtgcagc tttcgacgtt ggtgaccgga aacacgtacc gaaacccgac cctgctcgcc
     1921 aaggaaatca ccacgctgga tgtggtcagt ggcgggcgcg cgatcttggg tctcggtgcc
     1981 ggctggttcg aactcgagca ccagcagctg ggcttcgaat ttggttcgtt cacagagcga
     2041 ttcgaaaagc tggaggaggc gcttcagatc gtcatcccga tgacccacgg tgagcggccg
     2101 agtttttcag gcaagtggta ccacaccgaa aacgccatca acgagccccg ctaccgcgac
     2161 cacattccgg tgatgctcgg cggcagcggc gagaagaaga cgttccggct cgccgcccaa
     2221 tacgccgacc acctcaacat catcgcgcca atggacgaac ttccaggcaa actcgttgcg
     2281 ctggaacaac gatgcgcgga ggccaaccgc gaccccgcga cgctggaaac gagctgcctg
     2341 ctgaccgtgg tgctcgacgg atacgaatca catggagcga tcgatgaagc ccgcggtggc
     2401 cgggcggtca gtggctcacc acagcaagtg gcggaggaga tcaagcgtcg cgtgctcgat
     2461 gtcggcgtgg acggcgtcat cgtcaacttg ccgacccatc gctatacacc cggagtgata
     2521 acgaaagtcg gcgaagtgct gcgcagcatc agttcttgat cgcactgacc aggctgatgt
     2581 tgccggtcat tgcgcccgct tcggactcgg gatcgggaac gggccgatcg agcgagcgca
     2641 gctgagacgg caacggcgtg gccacggacc gccagccgcg gtggccgaac cattcgtggg
     2701 cgggcgcgtg ctgctcgttg tagacgagcg tgaagaaggt gtcctcatcc cctgcagcgc
     2761 gttcctcctc tcgcttcgcc tcgaaaacat cggcgtccag cggcaccgct tcctcgaccg
     2821 ccaaatggct gcccggcgcg gccagcgagt cgatgccggc gaacagctgg tgctgagcgt
     2881 cggccggcag gtagatgagc aacccttcgg cgatgaacgc cgacggtttc gacggatcga
     2941 acccgttgtc acgcaatgcc tgtggccagt cgttgcgcag atcgacggcc acctctctgc
     3001 gctcagtagt gggcgtgtcg ccatgcgcgg ccaacacctc gcgcttgaac tcgagcaccc
     3061 gcggctggtc cagctcgtac accacggtgc cgtcgggcca tgtcagccgg tagccgcggg
     3121 agtcgagccc ggccgcaagc agcacgatct gccgcacccc ggccgcggcc gcggcttgaa
     3181 agtaggtgtc gaagtatcgc gtccgagcgc cctggaagtt gacgaagtgt tcgccgaact
     3241 cggtcttcag cgggtggtcg ggggcgccgc cgtcgagcag gtcggcccat tcgccgccga
     3301 ccgcccggca gaacacctcg gcatacggat cgaccgccgc gggatcgggt ttctgggctt
     3361 ccagggcccg cgccgcggcg acgaacagcg cggtcgaacc gacactcgtc ttgatgtccc
     3421 agctgtcatt atcggtacgc acgtacgcgt ttatacgcgc tgattctgac aacccggcca
     3481 gactggatac atggcggaac cgtggaacat caacatccac tacgacggca aactcgaccg
     3541 ctgtgtgcca ccggaggtgg ggtccgtgct cgacgttggc tgcggcgacg gttttttggc
     3601 cgctcgtctg tcgcaacgca ttccgacggt cgtcgctgtg gacatcgatc aacccgtcct
     3661 ggcgcgagcc aggcaacggt ttcccagcgc gccggtgaag tggcggtgcg gtgacatcct
     3721 cgacatcgct gacgatttgg gcgccttcga cgccgtggtc tccaatgcga cgctgcatca
     3781 tctccccgat acccgggtcg ccctgcggcg gctgggcagc ctcgtcacgc ccggcggaac
     3841 gctcgccctg gttacgttcg cccgcccggg gtggcgccac ttgccatggg cgatggccac
     3901 actcgtgatc cgcggggtcg cgatccggtt gcgcggcaaa tgggaacaca gcgcgcccac
     3961 tgtctggccg ccgcgctaca ccgtcgccca gttacgtcgt gatgtccgtg cggaactacc
     4021 aggcgcgcgg gtgtcactgc tgccgctggg gcgagtcttc atccagtggc aagcaaccgg
     4081 ttgactacac cagaggacgt ccggtacgga tcctccaatc gaggtccttc aacagcacgt
     4141 tgaaggggaa ctgacggatg aaccgcggca gcaggtgatt gaccgtccgg agcgcacgca
     4201 tcaatcggtc gaaccgccgc tgtctcaccg cgtcccacgg caaccgcatc tcgtcgcgga
     4261 accgttgcgg cagaaagccc gtcgtgatca gcaggctgaa gttgtccagc gcccgctgga
     4321 tcggaccggg cagcttgacg ccgcgcacgc gggccgccgc gatcggccaa aggtattcgc
     4381 gcaccgcgtc gtcgatgtgc accttctcca ccgattcccg ccagtaccga tcgaatgcgg
     4441 cgcggtcctt cggccacata tcctccggaa cctgaagcgt ggtgccgagc ggcaccccct
     4501 cgcgatagtg ccgctcggcg gtctcctcat ccatctcgcc gatgaacagc cggtacacgt
     4561 cgaccgcacc cttgtacagg cacgcgccga cccacagctg taggtctttg tcgaagccgt
     4621 ggtacttcac cgggctctcg tcggtcgaat acacctgagc gtgcgcgcgg ttcaccgcac
     4681 gccgaaacgc cgccttctgc tccggggaac ctttggtcgc caccgcgagg taggtgaaag
     4741 tggtacgcgc tcgcttgatc ggatgcaggt caacccggcc gctctcgacg cgactctcca
     4801 tcacgccgta gccgacgccc ggccgagcca gctgcatgat cacgttggcg ggcccggcca
     4861 gcagcgccac acccatcagg ccgtcgtcga agttcgcacc acggcgccgc gatgtcgtcg
     4921 gcagcgccgc ggcaggctcg cttaccgccc gttcgacgtg ctgcaccggc tcactgatcg
     4981 tcattgaccc cacccatcgg caaatgtgag aacatatgtt tccagatata ttgccctcac
     5041 gacgacggcg gtgtcaagat ggatccgatg gctcacgttc ggccctaccg cggcattgag
     5101 gcggccgacc gggtcgcgga gcgtcggcgc cgcttcctgg aggcgggcct ggacatcctc
     5161 ggttccgacc ccgacctgtc cgaactcacg gtgcgcggca tctgccgcga ggccgggttg
     5221 gcgctgcggt acttctacga gagcttcacc gacaaagacg atctcgtcgc cgcagtgttc
     5281 gactgggtga tcgccgatat cgccgcaacg acgcaggcgg cggtcgcggc agcgcccgtc
     5341 aaggaacaga cccgggccgg catcgccaac atcgtgcgga gcgtcgctgc cgacactcgg
     5401 gtgggccatc tcctgttcag tccgcagctg gccaattcgg tcgtactgcg taagcgcgcg
     5461 gaatccttcg cgttgatggc aatgctgaca ttccagcacg cggccacttt gggtgcgaaa
     5521 gacaccgacc tcgtcaaggc agcgtcgcat ttcgtggtcg gcggcgtcat gcagatgatc
     5581 gccgcgtggc ttgcaggtga cgtcaggatg gacaccgacc agctcgtcga tcagctggcc
     5641 gcgatgatcg atgcgctggc cgtcccagag ctctaccggg gctagcgacc agcgggcact
     5701 cggggtcgac gatccgcacc ggtcttcggc gcggtcttcg tgtcgcgctc ggtgaattct
     5761 ttggtccagc aagcgaactc gagcgtgatg ccgtccggat cgtggaagta gaacgaccgc
     5821 acgtatacgc cgggatgcac cgtcgccgac acctgcgcct cgctctcgtc atggttgagc
     5881 accgggccga cgcgcacacc cttgtccttg agccgctgcc ggtactcgtc gaacttttcc
     5941 gcgggcacgt gaaaagccag gtggttcatg gtgctgacgg cgctcacgat gtcaccgatg
     6001 cccgggatcg cttcgggcga cgagacgcag ggcacccggt cgggtgcgtc tgcgaaccag
     6061 aagaacgcca cgcagtcgcc attgcctgcg tcgaagaaga agtgctgtcc catgccgccg
     6121 ggtagatcca gcgacttgat cagcggcatg ccgaggacat tgctgtagaa gtcgacggtg
     6181 cgcgccatgt cggagcagac cagcgccacg tggttgatcc cgccgagctc gaattcggaa
     6241 ttgggattgt ctggcttgat cattcgcagc tcctctagcc aagctcgaaa tctgaatcta
     6301 acatcagatt caggtgcgat caatggccga ggagcttcgt gaccgtcagc ccccgcgaac
     6361 aattgccgac tctgcgtggc aggcagacgc aggccgcgat cgacgcggcg gccagggcgg
     6421 tgatcgcacg caagggcatt ctggcgacga cgatttcgga catcgcggcc gaagcagaca
     6481 gatccacggc gtcgttctac aactattacg actccaagga agcgatggtc cgcgagtggg
     6541 ccgtgcgatt ccgcgacgag gcgcgagaac gctcgcgcgc ggcggcagaa cccgggctga
     6601 ccaactggga gcggtcgtat caggccgcgg ccgcccactg gaacacctac cgcaatcggt
     6661 tggccgagat gatcagcgtg tcgcagttgg ccatggtgag cgacgatttc gcccagtact
     6721 ggagtgacat ctgctcgcta ccaatcggct tgatcacaca gattgtgaaa cacgcccaac
     6781 gccaaggctt ctgcgctgct gacgacgatc cccaactgat cgcggaggcg atggtgtcca
     6841 tgctcaacca gttctgctac acgcggctgg ccggaaacat ggcggctgcc gtcgatgacg
     6901 acgcgtgcat caccacgttg gccaacattt tctaccggac gatctatccc aaggaggccg
     6961 ggcttttatg accaaaacac cggcgtcatc cgggacaccg ggcgtcaccc gcgagttcgt
     7021 cgggacggat tcgccgacgg cacgacgtgc cgggtccggc gggcatccgt gtcagggcct
     7081 ctaccaccgc ggcgtcgggc gcaagccaaa ggtcgcgatg atcgccacgc actaccaaat
     7141 cgacttctcc gagcactatc tcgccgatta catggccacg cgcggcatcg gtttcctcgg
     7201 ctggaacacc cgctaccgcg gatacgagag cagcttcctg ctcgaccacg cgctggtcga
     7261 catcggcatc ggggtgcgct ggctgcgcga agtccaaggg gtggaaacca tcgtgctgct
     7321 gggtaattcc ggcggcggct ccctgatggc tgcctaccag gcgcaggccg tcgacccaca
     7381 cgtgacgccg ctggagggca tgcggcccgc ggccgggctg accgatttgc cgcccgctga
     7441 cggatacatc gccagtgcgg cgcatcccgg tcgtccggat gtgctcaccg catggatgga
     7501 cgcgtccgtc gtcgacgaaa acgacgccat tgcaagcgat cccgacctcg acctgttcaa
     7561 cgaacacaac ggccccccgt accccaagga gttcctcgag cgctaccggt cggcacaagt
     7621 agcccgcaac cacgcgatca ccgactgggt cgagacggag ctcaagcggg tgcacgcggc
     7681 gggcttttcc gaccgtccct tcacggtgct gcgcacgtgg gcggacccgc ggatggtcga
     7741 tccgacgatc gagccgacga agcgcccggc caatatgtgc tacgtcggcg ttccggtcag
     7801 ggcgaaccgc tcggcgcatg gcatcgccgc cgcgtgcacg ctgcgcaact ggctgggtat
     7861 gtggagcctc cggcacgcgc agacccgcgc cgaaccgcac ctgtcccgca tcgaatgtcc
     7921 tgcgttggtg atcaacgccg agcaggacac gggcgtgtac cccagcgacg ctcagcgcat
     7981 ctacgacgcg ctggccagca ccgacaagtc tgcgtgctcg atcgacaccg accactactt
     8041 cactaccccg ggcgcccgca gcgagaaggc cgatacgatc gccaagtgga tcgcgaagca
     8101 ctggcactga gagtcctcgc gcacttcacg ccgggcgaca aagtcaccga attccttgca
     8161 ccagagtcgg attggctcga cattcgctac tgcgccgagg acgatgacga caccttctac
     8221 cacgagctgc ccgaggcgga ggttatctgg cacgtgctaa gacccatctc gggccacgac
     8281 ctcgagaagg cgcaacggtg caagctcgtg cacaagatgg gtgcgggcgt gaacaccatc
     8341 gacgtcgccg cggcgactcg gctcggcatc gcggtgggga acatgcccgg tgcgaacgcg
     8401 gcctcggtcg ccgagggcac cgtgctgttg atgctggccg cgctgcgacg gcttcccgcc
     8461 ctggacaggg cgacccgcga aggcaggggc tggccgtcag atccgagtct cggcgaaaca
     8521 gtgcgcgaca tcggcggctg caccgtcggg ctggttggct acggcaacat cgccaagacc
     8581 gtcgagacga tggtgaccgc tatgggtgca cgagtcctgc acaccagcac gcgagacgac
     8641 ggccatcccg gctggcgccc gctccctgag ctgctcaccg aaagcgacat cgtctcgctg
     8701 catctgccgc tgaccgataa gaccgcaggc atgctggatc gcgaagccct cgccacgatg
     8761 aagaccgacg cggtgctcgt caacacctca cgcggcggcg tggtcgacga ggacgcattg
     8821 gtcgacgcac tgcgcaccgg cggggtgggc gccgcgggcc tggacgtgtt cgctgtcgag
     8881 ccaatatcga ccgacaatcc gctgctcggc ctggacaatg tggtcctcac gccgcacgtc
     8941 acctggtaca ccgtcgacac catgcggcgg tacctggaac atgcggtgga caactgccgg
     9001 cggatcaggg atgggcagga gctggccaac gtcgtcaacg agtagtctgg ctcccgaccg
     9061 accggttaat agggaaggct tctacgaaga ggtagctatg cccatcgcga tcactcctga
     9121 gcacaacgac ctggccgact cggtgcgctc cctggtggcg cgggtggcgc cgtcggacgt
     9181 gctgcacgaa gccctcgaaa ccccgatccc gaacccgccg acctactgga aggccgcggc
     9241 cgagcagggc ctgcagggcg tgcacttggc ggaatccgtt ggcggacaag gcttcggcat
     9301 cctcgagctg gccatcgtgc tggccgagtt cggctacggc gcggtgccgg g
//
LOCUS       DASRJA010000518        10827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3686830,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000518 DASRJA010000000
VERSION     DASRJA010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10827
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3686830"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFC01_22600"
     CDS             complement(<1..740)
                     /locus_tag="VFC01_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014988616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN82441.1"
                     /translation="MSARIGVVFRPDFRPDTLRQTAAAAEHAGVTELWLWEDCFLQGG
                     IAQAAVALASSETLTVGLGVLPAPLRNVVTTAMEIATLESMFPGRIRVGLGHGVQDWM
                     RQAGAAVASPLTLMREYVTALKDLLAGRTVTVDGRYVRLADVCLDWAPQQRVPVLIGA
                     TGPKTLALAGETADGVILDSQYTVTTLRDPLGHVASGRAARGGTPFSTVLFLACGADQ
                     AAVSVGPYLEAGVDTLVFQPVGAQAVMP"
     gene            complement(751..1545)
                     /locus_tag="VFC01_22605"
     CDS             complement(751..1545)
                     /locus_tag="VFC01_22605"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZN82442.1"
                     /translation="MAPGSPRKAAVLGSPIAHSRSPQLHLAAYRALGLDGWTYDRIEC
                     TASELPGLVAGFGPEWVGVSVTMPGKFAALRFADERTARAELVGSANTLVRTQAGWRA
                     DNTDIDGVKGALGRAAGRAAVLGSGGTAPAVVVGLAELGVQDISVVARNWDKARPLVE
                     LGTRVGVYARWVELGIPLVDVDIVVSTIPADVAARHVDSVVATPLLLDAIYEPWPTPL
                     AAAVAAAGGQVVSGLQMLLHQAFAQVEQFTGMPAPKEAMKAALAEQ"
     gene            complement(1532..2776)
                     /locus_tag="VFC01_22610"
     CDS             complement(1532..2776)
                     /locus_tag="VFC01_22610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZN82443.1"
                     /translation="MTDNWGRGRAEPVAVGPPRRRMTRADRVREARSRRKRRFASGFA
                     LALLIVIVVGAVFLGSKMWHTLFGAANDYTGDGVNDVVIQIHDGDSTTAIGKTLEDRK
                     VVADSKVFVEAAEGNSAIAAIQPGFYKVRTKIPASNAVARLADPQNRVGKLVIPEGRQ
                     LDDIQDVKTNAVTEGVFSLISRATCVNLDGDRHCLSVDDLRKAAGSAAPSALSVPEWA
                     INPVSALGNDHRRLEGLIAPGTWNIDPSASAQDILSTLIGTSATQYAQGGLLDTAKAM
                     NMSPYQILTVGSLVQREATPEDFAKVARVIYNRLHENRTLEFDSTVNYPLDRIEVATT
                     DADRAQHTQWNTYVRPGLPATPICSPGQPALAAAEQPAPGDWLYFVTVDMQGTTLFTR
                     DYEQHLANIELAKRNGVLDSAR"
     gene            complement(2769..3284)
                     /gene="ruvX"
                     /locus_tag="VFC01_22615"
     CDS             complement(2769..3284)
                     /gene="ruvX"
                     /locus_tag="VFC01_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZN82444.1"
                     /translation="MADIDDRLPDRPGGDDPGRGRRLGVDVGTVRIGVAASDPDGILA
                     TPVETVERDRRNKSGKHLRRLAQLADEYEAVEVVVGLPRTLADRSGSSARDAVELADQ
                     LARRIAPTPVRLADERLTTVAAQRSLRDAGVRAKGQRAMIDQAAAVGILQNWLDQRRA
                     ALTAHGEVGDD"
     gene            complement(3288..5981)
                     /gene="alaS"
                     /locus_tag="VFC01_22620"
     CDS             complement(3288..5981)
                     /gene="alaS"
                     /locus_tag="VFC01_22620"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZN82445.1"
                     /translation="MQTHEIRKRFLDHFVKAGHTEVPSASVILDDPNLLFVNAGMVQF
                     VPFFLGQRTPPYNTATSIQKCIRTPDIDEVGITTRHNTFFQMAGNFSFGDYFKRRAIE
                     LAWTLLTNPIDQGGYGFDPERIWATVYLDDDEAVGLWQEVAGLPADRIQRRGMADNYW
                     SMGIPGPCGPSSEIYYDRGPEFGIEGGPVANEDRYIEIWNLVFMQNERGEGPAKEGYE
                     ILGPLPRKNIDTGMGVERVAFLLQGVHNVYETDLLRPAIDTVAARAPRGYDVGNHEDD
                     VRYRIIADHTRTAAILIGDGVSPGNDGRGYVLRRLLRRVIRSAKLLGIDDPIVGDLMA
                     TVRDAMGPSYPELVTDFERILRIAVAEETAFNRTLASGSRLFEDAASATKASGAKVLS
                     GTDAFTLHDTYGFPLELTLEMAAEAGLSVDEEGFRSLMAEQRQRAKADAAARKQAHAD
                     LSEYRELVDDGPTEFTGFDELSSEAKILGIFVDGKRVPVVAHNGGPAPDRVELILDRT
                     PFYAESGGQIADEGSITGAGASGSAKAAVSDVQKIAKTLWAHRVNVESGEFVEGDTVI
                     AAVDPRWRHGATQGHSGTHMVHAALRQVLGPNAVQAGSLNRPGYLRFDFNWQGPLSEG
                     QRTQIEEVTNEAVEANYQVNSFITELDKAKAMGAMALFGENYPDEVRVVEIGGPFSLE
                     LCGGTHVESSAQIGPVTILGESSVGSGVRRVEAYVGLDSFRHLAKERALMAGLASTLK
                     VPSEEVPARVANLVERLRAAEKELDKMRLANARAAAANAAAGAEQIGKVRVVAQRMAG
                     GMSAADLRTLVGDIRGKLGSEPAVVALIAEGENDSVPFVVAVNPAAQDLGLKANELVK
                     QLGAAVNGRGGGKADLAQGSGKGAAGIDAALAALRAEIGRS"
     gene            complement(6094..6483)
                     /locus_tag="VFC01_22625"
     CDS             complement(6094..6483)
                     /locus_tag="VFC01_22625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZN82446.1"
                     /translation="MLDIDTSRRRIVDLTDAVRSFCGEHGDGLCNVFVPHATAGVAII
                     ETGAGSDDDLIDTLERLLPRDDRYRHEHGSPGHGADHVLPALVAPSVTVPVQGGQPLL
                     GTWQSVVLVDLNRDNRQRQVRLSFVNG"
     gene            6669..7292
                     /locus_tag="VFC01_22630"
     CDS             6669..7292
                     /locus_tag="VFC01_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82447.1"
                     /translation="MHARSTTISAQASSIDDGIAYMRDDLMPELENIDGYVGISLLVD
                     RDSGRCIITAAYESEDAMHAAADKAKQLRSQAAERFGGDVQKIEEWEIGCLHRDHQSA
                     DGACVRATWVKADPALADQNIEFFKTSVLPDLENLEGFCSASLLIDRSTGVGVSSITF
                     DSRESMERNREQATALKAAKIREVGVEELDECEFELALAHLRVPEMA"
     gene            7401..8027
                     /locus_tag="VFC01_22635"
     CDS             7401..8027
                     /locus_tag="VFC01_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZN82448.1"
                     /translation="MFARSTTIQAQPSSIDAGIAHVRDEIMPAVQAMDGCVGISLLAD
                     RDTGGCIVTTSWETEDAMRASRERVRPLRDRAAEAFGSGGDTQVDEWEIAVLHRDHRS
                     ADGACVRATWIKVPPDQADRAIEFYKDSVLSSLENLEGFCSASLLINRSSGRAVSSAT
                     FDSRDAMERNREQAKELRNTRTRELGADVLDVGEFELALAHLRVPEMA"
     gene            8112..8417
                     /locus_tag="VFC01_22640"
     CDS             8112..8417
                     /locus_tag="VFC01_22640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZN82449.1"
                     /translation="MTITGVITAILIGIVVGVLGRLLVPGRQPIGWLVTIAVGIVSAF
                     IGTAIARALGIPTATEGIDWLELLCQVVVAAIGVALVSALLGRRTGFMGRRRSGLMR"
     gene            complement(8489..8884)
                     /locus_tag="VFC01_22645"
     CDS             complement(8489..8884)
                     /locus_tag="VFC01_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN82450.1"
                     /translation="MDWTLEVVVVPVSDVDRAIAFYRDQLGFNLDHDTRAGDQRFAQL
                     TPPGSGCSIVLSDPSPMQPGALKGVQLVVADAYRAREQLLESGVAAGDIDVIDERDGG
                     TLFGFTDPDGNSWLVQQLKARADKPLLPR"
     gene            complement(8884..9357)
                     /locus_tag="VFC01_22650"
     CDS             complement(8884..9357)
                     /locus_tag="VFC01_22650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN82451.1"
                     /translation="MTNPLNLNAPVDTLAMEFTRDFDAPVEALFRAHAEPDLVKQWLG
                     PRDLEMTIEHWDFKTGGGYRYVHKADRGAFSFNGVFHKVRDNEFIIQTFEFEGAPDMV
                     NIEFMWFEVLGNGRSRLRGRSICPNIEARDGLLSSGMEGGMREGYEKLDELLKSL"
     gene            complement(9354..9665)
                     /locus_tag="VFC01_22655"
     CDS             complement(9354..9665)
                     /locus_tag="VFC01_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN82452.1"
                     /translation="MALADPVRRAIVARLSRGPATVNELAAPFDITKQAVSKHIQVLE
                     QAGLVTRTRDAQRRPVHLDAAALERLTAWIDRYRLDAERSYRQLDALLAAMTDKEGTE
                     K"
     gene            complement(9789..>10827)
                     /locus_tag="VFC01_22660"
     CDS             complement(9789..>10827)
                     /locus_tag="VFC01_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZN82453.1"
                     /translation="KDVRAVIDEARSLRRTGGNATVLFIDEVHRFSKTQQDALLSAVE
                     NRIVLLVAATTENPSFSVVAPLLSRSLILQLQPLDADAVRTVVRRAIDDPRGLGGQVD
                     VTEDAVELLVQLSAGDARRALTALEVAAEAGEQVTVATIEQSLDKAAVRYDRDGDQHY
                     DVISAFIKSVRGSDVDAALHYLARMLVAGEDPRFVARRLMILASEDIGMADPTALQTA
                     VAAAQTVQLIGMPEAQLTLAHATIHLATAPKSNAVISAIGAAMSDIRDGKAGLVPAHL
                     RDGHYSGAAKLGNAIGYKYSHDDPDGVVPQQYPPDELVGVDYYQPTTHGAEREIATRL
                     DKLRAIIRKKR"
BASE COUNT         1776 a         3648 c         3497 g         1906 t
ORIGIN      
        1 tcgggcatca cagcctgcgc ccccactggc tggaatacga gggtgtccac ccccgcctcg
       61 agatagggcc cgaccgagac tgcagcctga tcagcgccgc atgcaagaaa cagcaccgtg
      121 ctgaagggcg tcccgccgcg ggcggctcgt cccgacgcga catggcccag aggatcccgc
      181 agcgtggtaa cggtgtactg agagtcaagg atcacgccat ccgcagtctc accggccaat
      241 gccaacgtct ttggtccggt cgcgccgatc agcaccggaa cccgttgttg cggagcccaa
      301 tccaaacaca cgtcggcaag gcgaacgtag cggccgtcaa cggtgacggt ccgcccggcc
      361 aagagatcct tcaaagccgt gacgtactcg cgcatcaatg tcaggggtga cgccacggcg
      421 gcgcccgcct gccgcatcca gtcctgcaca ccgtggccga ggccaacgcg tatccggcct
      481 gggaacatcg actcgagggt ggcgatctcc attgcggtgg ttaccacgtt gcgcagtggt
      541 gctggcagca ctccgagacc gaccgtgagc gtttcagagg acgccaacgc caccgccgcc
      601 tgagcgatgc cgccctgcag gaagcagtcc tcccacagcc acagttcggt gacgccggca
      661 tgctcggcgg ccgcggccgt ttgtcgcaaa gtgtcgggac ggaagtctgg gcgaaacacc
      721 acgccgatgc gagcactcac gcgtccgaca ctactgttcg gccagcgccg ccttcatcgc
      781 ttccttcggc gcaggcatcc cggtgaactg ctcgacctgg gcgaatgcct gatgcagtag
      841 catctgcagc ccgctgacga cctgtccgcc tgccgccgcg acggcggccg caagcggggt
      901 cggccatggc tcgtagatcg catcgagcag cagtggcgtc gctaccacgc tgtcgacatg
      961 tcgggccgcg acgtcggcgg ggatcgtgct gacgacgata tcgacgtcga ccagcgggat
     1021 tcccagttcg acccagcggg cgtaaacgcc cacgcgggtc ccgagttcga cgagcggccg
     1081 tgccttgtcc caattccttg cgacaaccga aatgtcttgc acgccaagct cagcgagccc
     1141 cacaacgacc gcaggcgcgg tgccacccga gcccagcaca gccgccctgc cagctgcccg
     1201 tccgagcgca cccttcacgc cgtcgatgtc ggtgttgtcg gcccgccagc cggcttgcgt
     1261 ccgcaccagt gtgttagccg agcccacgag ttccgcgcgt gcagtgcgtt cgtcggcgaa
     1321 ccgcagtgcc gcgaacttgc ccggcatcgt caccgacacg cccacccact ccgggccgaa
     1381 acctgctacc aaaccaggca attcggatgc ggtgcattcg atgcggtcat acgtccagcc
     1441 gtccagcccg agcgcgcgat aggcggccag gtgcagctgc ggtgatcggg aatgcgcgat
     1501 cggtgagccg agcaccgccg ccttgcgcgg actacctggc gctatcgagg acaccgttgc
     1561 gcttggccag ttcgatgttc gccagatgct gctcgtagtc gcgggtgaac agcgtggttc
     1621 cctgcatatc gaccgtcacg aagtacagcc aatcccccgg cgcgggctgt tcggccgccg
     1681 ccagcgccgg ctgcccgggt gagcagatcg gggtagccgg aagaccaggc cgcacatagg
     1741 tattccattg cgtgtgctgg gcgcggtcgg cgtcggtcgt cgccacctcg atccggtcca
     1801 acggatagtt cacggtcgag tcgaactcca aagtccggtt ctcatgcagc cggttgtaga
     1861 tcacccgcgc aaccttggcg aagtcctccg gggtcgcttc ccgctgcacc agcgacccga
     1921 cagtgaggat ctggtacggc gacatgttca tcgccttagc cgtgtcgaga agcccgccct
     1981 gtgcgtactg cgtcgcgctg gtcccgatca gcgtggacaa gatgtcctgg gccgatgccg
     2041 acggatcgat gttccaggta cccggcgcga tcaacccctc cagccggcgg tggtcgtttc
     2101 ccagcgcgct gaccgggttg attgcccact ccggcaccga aagcgccgac ggcgcagcgc
     2161 tccccgcggc cttgcgcagg tcgtccaccg acaggcaatg cctgtcgccg tcgaggttca
     2221 cacacgtcgc acgcgagatc aggctgaaga cgccctcggt gacggcgttg gttttgacat
     2281 cctggatgtc gtcgagctgc cgtccttctg gtatcaccag cttgccgacc ctgttctgcg
     2341 gatcggcaag tcgcgcaacg gcattggacg ccgggatttt ggtgcggacc ttgtagaagc
     2401 cgggctggat tgcggcgatg gcggagttgc cctcggcggc ctcgacgaac accttgctgt
     2461 ccgcgaccac cttacggtct tccagcgtct tgccgatcgc tgtggtggag tcgccgtcgt
     2521 ggatctggat gaccacgtcg ttgacgccgt cgccggtgta gtcgttggcc gcaccgaaca
     2581 gcgtgtgcca catcttcgaa cccaggaaca ccgcaccgac gacgatcacg atcagcagcg
     2641 ccagcgcgaa gccactggcg aagcgccgct ttcgccgact tctggcttcc cgcacgcggt
     2701 ccgcccgggt catcctgcgc cgcggcggtc cgaccgccac gggttcggcg cgtccgcgac
     2761 cccagttgtc agtcatcccc gacctctccg tgcgcggtca acgccgcgcg ccgttggtcc
     2821 agccaattct gcaaaatccc cactgccgca gcctgatcga tcatcgcccg ctggcccttg
     2881 gcgcggacgc ccgcgtcccg aagcgaccgc tgcgccgcga ccgtggtgag ccgttcgtca
     2941 gccaatcgca ccggtgtcgg cgcgatgcgt cgggccagct ggtcggccaa ctcgactgcg
     3001 tccctggcag acgaaccgga ccggtcggca agggtgcgcg gcaagcccac caccacctcg
     3061 acagcttcgt actcgtcggc cagttgggcg aggcggcgca ggtgtttgcc ggacttgttc
     3121 cgtcggtccc gctcgaccgt ttccaccggt gtcgccagga tcccatccgg atcgctggcg
     3181 gccaccccga tgcgcaccgt accaacgtcg acaccgagcc gtcgaccgcg gccgggatcg
     3241 tcgccccccg gccgatccgg tagacggtca tcaatatcgg ccaccggcta gctccggcct
     3301 atttctgcgc gtagcgcggc caatgccgcg tcgatacccg ccgccccctt accggaaccc
     3361 tgtgccagat cggccttgcc gcccccacgg ccattgaccg ccgcacccaa ctgcttcacc
     3421 aactcgttgg ccttcagccc gagatcctgc gcggccgggt tgaccgccac tacgaacggg
     3481 acgctgtcgt tctcaccctc ggcgatcaac gcgacgacgg cgggctcgct gcccaacttg
     3541 ccccggatgt caccgacgag cgtgcgcaga tccgccgccg acattccgcc ggccatccgc
     3601 tgcgccacga cacgcacctt accgatttgc tcggcgcccg cagcggcatt cgctgcggca
     3661 gcccgcgcat tcgccagccg catcttgtcg agttcctttt cggcggcgcg aaggcgctcg
     3721 accagattgg ccacccgggc cggaacttcc tccgacggga ccttcagcgt cgaggccaga
     3781 cccgccatca gggcgcgttc cttggccaag tggcggaacg aatccaaacc gacgtaggcc
     3841 tcgacgcggc gcacgccgga cccaaccgac gactcgccga gaatcgtcac cgggccgatc
     3901 tgtgccgagc tctccacgtg cgtgccacca cacagctcga gcgagaacgg cccgccgatc
     3961 tcgaccaccc gcacctcgtc ggggtagttc tcaccgaaga gcgccatcgc gcccatcgcc
     4021 ttggccttgt ccagttcggt gataaagctg ttcacctggt agttggcctc gacagcttcg
     4081 ttggtgactt cctcgatctg cgtccgctga ccctcgctca gggggccctg ccagttgaag
     4141 tcgaaccgca aataacccgg ccggttcaag gagccggcct gaacggcgtt ggggcccagc
     4201 acttgtcgta gcgcggcgtg caccatatgg gtgccggagt ggccctgtgt agcgccgtgc
     4261 cgccagcgcg ggtcgacggc ggcgatgacg gtgtcaccct cgacgaattc gccggactcg
     4321 acgttgacgc ggtgcgccca cagggttttg gcgatcttct gcacatcgct gactgccgcc
     4381 ttggccgagc cagacgcgcc ggcgccggtg atgctgccct cgtcggcgat ctgaccaccc
     4441 gattcggcgt agaacggcgt gcggtccagg atcaactcga cgcggtcagg cgccggacca
     4501 ccgttgtgtg cgaccacggg gacccgctta ccgtccacga agattccgag aatctttgcc
     4561 tcggaggata attcgtcgaa gccggtgaac tccgtcgggc catcgtcgac tagctcgcgg
     4621 tactccgaca gatcggcgtg cgcctgtttg cgcgcagccg cgtcggcctt cgcacgctgg
     4681 cgttgctcgg ccatcaggct gcggaagcct tcttcgtcga cgctcaggcc agcctcggcg
     4741 gccatttcga gcgtcaattc cagtgggaag ccgtatgtgt cgtgcaacgt gaacgcgtcg
     4801 gtgcccgaca gcaccttcgc acccgacgcc ttggtcgcgc ttgcggcgtc ctcgaacagc
     4861 cgcgagcccg aggccaaagt ccggttgaac gccgtctcct cggcgaccgc gatgcggagg
     4921 atccgctcga agtcggtgac gagttcgggg tacgaagggc ccatcgcgtc gcgcacggtc
     4981 gccatcaggt cgccgacgat cgggtcgtcg atgccgagca gcttggccga gcgaatcacg
     5041 cggcgcagca gccgccgcag cacgtagccg cggccgtcgt tgcccgggct caccccgtcg
     5101 ccgatcagga tcgcggcggt gcgggtgtgg tcggcgatga tccggtagcg aacgtcgtcc
     5161 tcgtggttgc cgacgtcgta gccgcgtggg gcgcgcgcgg ccaccgtgtc aatggccggg
     5221 cgcagcaggt ctgtctcgta gacgttgtgc acgccctgca gcaggaacgc cacccgctcc
     5281 acgcccatgc cggtgtcgat gttcttgcgc ggcagcgggc ccaggatctc gtagccctcc
     5341 ttggccggcc cctccccccg ctcgttctgc atgaacacga gattccagat ctcgatgtag
     5401 cgatcctcgt tggcgacggg gccgccctcg atgccgaatt cggggccgcg gtcgtagtag
     5461 atctccgacg acgggccgca cggcccgggg atgcccatcg accagtagtt gtcggccatc
     5521 ccgcgacgct ggatgcggtc agccggcaac cccgcgacct cctgccacag cccgactgcc
     5581 tcgtcgtcat ccaaatagac tgtggcccat atcctttcgg ggtcgaatcc gtagccgccc
     5641 tggtcaatgg ggttggtcag cagagtccat gccagctcga tggcgcgacg cttgaagtag
     5701 tcgccgaacg agaagttgcc ggccatctgg aaaaaggtgt tgtgccgggt ggtgatgccc
     5761 acctcgtcga tgtctggcgt ccggatgcac ttctggatgc tggtcgccgt gttgtacggc
     5821 ggggtgcgct ggccgaggaa gaacggcacg aactgcacca tgcccgcgtt gacgaacagc
     5881 aggttggggt cgtcgagtat caccgaggcg ctgggcacct cggtgtggcc cgccttcacg
     5941 aagtgatcga ggaaacgctt cctgatctcg tgtgtctgca cgtccctact gtcccttttc
     6001 tgcattacct gcgactattc gaccgcacta cagtaccggt ctgagtagcc ccactaaaaa
     6061 gtcaacgaac cggcgcggcg tcaagtgccg ggttcatccg ttgacgaagc tcagccgcac
     6121 ctgacgctgt cgattgtccc ggttcagatc gaccagcacg acgctttgcc aggtgccgag
     6181 cagcggttgg ccgccctgca ccggaaccgt gaccgacggc gccaccagcg cgggcagcac
     6241 atggtcggcg ccgtgccccg gtgatccgtg ctcatggcgg tatcggtcgt cgcgcggcag
     6301 caggcgctcg agcgtgtcga tgaggtcgtc atccgagccg gcgccggttt cgatgattgc
     6361 cacgccggcg gtggcgtgcg gcacgaaaac attgcatagc ccgtctccat gctcgccgca
     6421 aaacgaccgc accgcgtctg tgaggtcgac gatccgccgg cgcgatgtgt cgatatcgag
     6481 cacgtcggag ttcatgactt ccaccctacg gaccaccaat ttgcgagccg aaacatccct
     6541 gtttgcaaag cgaaatatcc cgattcctat tcgaccaatc cgctgaatcg gttggtcgcc
     6601 gggaggaagc tgagagtcgt ccggtggcgc caccggggcg ccgccccgac acaagggggt
     6661 gggtggacgt gcacgcacgc tctaccacaa tttcggcgca agcctcgtcg atcgacgacg
     6721 gaatcgccta tatgcgcgac gatctgatgc ctgaactcga aaacatcgac gggtatgtcg
     6781 gcatctcgct gttggttgac cgggactctg gccgttgcat catcaccgct gcctacgagt
     6841 cggaggatgc gatgcatgct gccgcggaca aggcgaaaca gcttcgcagt caagcggcgg
     6901 agaggttcgg cggcgatgtc cagaagatcg aagagtggga gatcgggtgc ctgcaccgcg
     6961 accaccagtc ggccgatggc gcctgtgtgc gggcaacgtg ggtaaaagcc gacccagctt
     7021 tggcggacca aaacatcgag ttcttcaaga cgtccgtgct gcccgacctg gagaacctcg
     7081 aagggttctg cagcgcgagc ctgctgatcg accgctcgac gggtgttggt gtgtcctcaa
     7141 tcacgttcga cagccgcgaa tcgatggagc gcaaccggga gcaggcgacg gctctcaagg
     7201 ccgcaaagat tcgcgaagtc ggcgttgagg aactcgacga gtgcgagttc gagttggcgc
     7261 tcgcccacct gcgcgtgccc gaaatggcct gacggagcaa tttgtcggat ccgccgccgc
     7321 gagccgctcg gcggcggagc gtagtgactg gaccggtggc gccgccgggg cgctgccccg
     7381 aacacttgag gaggcggatc gtgttcgcac gctctaccac tattcaggcg cagccctcgt
     7441 ccatcgacgc cggcattgcg catgttcgcg atgagatcat gcccgcagtt caggccatgg
     7501 atggctgtgt tggcatttca ctgttggccg accgggacac cggcggatgc atcgtgacga
     7561 cctcatggga gaccgaggac gccatgcgcg ccagcaggga acgggtacgg ccgcttcgcg
     7621 atcgggccgc ggaggcgttt ggatccggcg gcgacacaca ggtcgacgag tgggagatcg
     7681 cggtcttgca ccgtgaccac cgctcggccg acggcgcctg tgtgcgggca acgtggatca
     7741 aggtcccgcc ggaccaggcg gaccgggcca tcgagttcta caaggactcg gtcctgtcgt
     7801 ccctggagaa tctcgagggg ttctgcagcg ccagcctgct gataaaccgc tccagcgggc
     7861 gcgccgtctc gtcagcgacg ttcgatagcc gcgacgccat ggagcgcaac cgcgagcagg
     7921 cgaaggaact gcggaacacg cggacccggg aattgggcgc tgacgtcctc gacgtcggcg
     7981 agttcgagct cgcgctggcc catctgcggg tgcccgaaat ggcctgatta acgcccgtgc
     8041 gcattgacgc gggcgtttat tgacgacgcc gcaggggtat gtcacctcca gcccaatagc
     8101 ttggaggaac aatgacaatc accggcgtaa ttactgcaat tctcatcggc atcgtcgtcg
     8161 gcgtgctggg ccggttactg gttcccggtc gacagccgat cggctggttg gtgacgatcg
     8221 ccgtcggcat cgtgtcggcg ttcatcggca ccgcaatcgc ccgcgcgttg ggtatcccca
     8281 cggccacgga aggcatcgac tggctcgagc tgctctgcca ggttgtcgtc gccgccatcg
     8341 gcgtggcatt ggtgtcggcg ctcttgggtc gacggacggg ctttatgggg cgcaggcgtt
     8401 caggcctcat gcgctgaccg ctgcccgcgg tacccccggc gatcccggct ggcctccacc
     8461 agctgggatc gtcgtgttgg ggcctggttc agcgtggcag caagggcttg tccgcgcgcg
     8521 ccttgagctg ctggaccagc cacgaattgc cgtcggggtc ggtgaatccg aacaatgtgc
     8581 ccccgtcgcg ctcgtcgatg acatcgatgt cgccggccgc aactccactc tcaagtaatt
     8641 gctcgcgggc gcgataggcg tcggcgacga caagttggac tcctttgagc gccccgggct
     8701 gcatcgggga cgggtcgctg agcacgatcg agcagcccga gcctggtggg gtgagctgtg
     8761 cgaagcgctg gtcacccgcc cgggtgtcgt ggtccaggtt gaaaccgagt tggtcgcggt
     8821 agaacgcaat ggcccggtct acgtcgctga ccgggaccac aacgacttcc agagtccagt
     8881 ccatcagagg ctcttcagaa gctcgtcgag cttttcgtag ccctccctca taccgccctc
     8941 catgcccgac gacagcaggc cgtcgcgggc ctcgatgttg gggcagatcg accggccgcg
     9001 cagccggctg cggccattgc cgaggacctc gaaccacatg aactcgatgt tgaccatgtc
     9061 cggcgcgccc tcgaattcga aggtctggat gatgaattcg ttgtcccgca ccttgtggaa
     9121 cacgccgttg aaactgaacg cgccgcggtc ggccttgtgt acgtagcggt agccgccgcc
     9181 ggtcttgaag tcccagtgct cgatcgtcat ctccaggtcg cgagggccca gccactgctt
     9241 gaccaggtct ggctcggcgt gggcccggaa caacgcctcc accggagcgt cgaaatcgcg
     9301 ggtgaactcc atggccagcg tgtcgaccgg ggcgttgagg ttgagcgggt ttgtcatttc
     9361 tcggttcctt ccttgtcggt catcgccgcc agcagggcgt ctaactggcg gtagctgcgt
     9421 tcggcgtcaa gccggtaccg gtcgatccat gcggtgagcc gctccaacgc ggccgcgtcg
     9481 agatggacgg gcctgcgctg cgcgtcgcga gtcctggtga ccaagccagc ctgttcgagc
     9541 acctgaatgt gcttggacac cgcctgtttc gtgatgtcga acggggcagc cagctcgttg
     9601 acggtggccg gtccgcggga cagacgcgcc acgatcgcgc gccgaaccgg gtcggccagc
     9661 gccatgaagg cgcggtccag ccgggtgtct gcatccccac cactccccaa ttgtcaacct
     9721 tgtatttgat caatgaattg gttgaatacc aacgtagaag gctgtgcggc ggctgtcaag
     9781 gggtgatttc agcgcttttt gcggatgatg gcgcgcagtt tgtccagccg cgtggcgatc
     9841 tcgcgttcgg cgccatgagt ggtcggctgg tagtagtcga cgccaaccag ctcgtcgggc
     9901 ggatactgtt ggggcacaac accatccggg tcgtcgtggc tgtacttgta gccgatcgcg
     9961 ttgcccagtt tcgcggcgcc tgagtaatgg ccgtcgcgca gatgggcggg caccaggcca
    10021 gccttgccgt cgcggatatc gctcatcgcc gcgccgatcg cgcttatgac cgcattggac
    10081 ttcggcgcgg tggccaggtg gatcgtggcg tgcgccaacg tcaattgcgc ctccggcatg
    10141 ccgatcagct gtaccgtctg cgccgcggcg acggcggtct gcaacgccgt cgggtcggcc
    10201 atcccgatgt cctcgctggc cagaatcatc agccgacggg cgacgaaccg cggatcctcg
    10261 cccgcgacca gcatccgagc caggtagtgc agggcggcgt cgacgtcaga gccgcggacg
    10321 gatttgatga acgcgctgat cacgtcgtag tgctgatcgc cgtcgcggtc atagcgcacg
    10381 gcggccttgt ccagcgactg ctcgatggtc gcgaccgtca cctgctcgcc tgcctcggcg
    10441 gcgacttcca acgctgtcag agcgcgccgc gcgtcgcctg cagagagctg caccagcagc
    10501 tcgacggcgt cctcggtcac gtcgacctgg ccgccgaggc cgcgcgggtc gtcgatggcg
    10561 cgacgcacca cggtccggac ggcgtccgca tcgagcggct gcagctgcag tatcagcgac
    10621 cgcgacagca gcggcgcgac gacagagaac gacgggttct cagtggtggc cgcgaccagc
    10681 agcacgatcc ggttctctac cgccgacagc aacgcatcct gctgggtctt ggagaaccgg
    10741 tgcacctcgt cgatgaagag cacggtggcg ttgccgccgg tgcgccgcag gcttcgcgcc
    10801 tcgtcgatga ccgcccgcac gtccttc
//
LOCUS       DASRJA010000519         5690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3465829,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000519 DASRJA010000000
VERSION     DASRJA010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3465829"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFC01_22665"
     CDS             <1..329
                     /locus_tag="VFC01_22665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HZN82454.1"
                     /translation="IDRINAIANPQLPKIGERFLGTVVKTTDFGAFVSLLPGRDGLVH
                     ISKLGRGRRIAKVEDVVKVGDKLRVEIADIDNRGKVSLILVAEEDTADASSSADAPVD
                     AATASS"
     gene            307..1653
                     /locus_tag="VFC01_22670"
     CDS             307..1653
                     /locus_tag="VFC01_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZN82455.1"
                     /translation="MPQPPAARSRALHRGARADKTSEAVRRTTLPGGLRVVTEYIPSV
                     RSASVGVWVGVGSRDEGRSVAGAAHFLEHLLFKSTPTRTAVEIAQAVDAVGGELNAFT
                     AREHTCYYAHVLDTDLELAIDLVADVVLRGRCATDDVEVERAVVLEEIAMRDDDPEDT
                     LGDVFLSAMFGEHPVGRPVIGGVDSISAMTRAQLHSFHMRRYTPERMVVAVAGNVEHD
                     EVVALVRDHFGARLIRGRRPLAPRTGTGRVAGRPTLRMVHRDGEQTHLTLGVRTPGRH
                     WDQRWALSVLNTALGGGLSSRLFQEIRETRGLAYSVYSTVDTFSDSGALSIYAACQPE
                     RFDEVVRVTTEVLEKVARDGITEAECRIAKGSLRGGLVLGLEDSGSRMNRIGRSELNY
                     GEHRSIAHTLGKIEGVTLDEVNAVAHQLLTRQFGAAVLGPQRSKKSLPQPLRRIAG"
     gene            1670..2563
                     /gene="bla"
                     /locus_tag="VFC01_22675"
     CDS             1670..2563
                     /gene="bla"
                     /locus_tag="VFC01_22675"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HZN82456.1"
                     /translation="MPGLSRRQALLGGLTLAAAAACATKPVLADPEEPPPIGEHIAAL
                     ERRHDAYVGVYAVDLDGGRSISHRGQEAFAMCSTFKGYAAARVLQMVERDELTLDQRV
                     FVDPAAIVANSPRTAPRAGGDMTLDELCQAALQVSDNTAGNLLLKTIGGPSAITAFAR
                     SIGDPSSRLDRWETALNSAVPGDPRDTSTPEALGRGYRALLAGDALAPAQRQQLETWM
                     RANETSSMRAGLPPDWTTADKTGSGDYGTTNDAGIAYGPAGQRVLLAMMTRSQGNDPK
                     AQNLRPVIGELSAMLLPEVLA"
     gene            2674..3366
                     /locus_tag="VFC01_22680"
     CDS             2674..3366
                     /locus_tag="VFC01_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZN82457.1"
                     /translation="MAIETIALHDYLPPEEPNGAVAVIDEHEAIHAAVELWCNQAQIG
                     FAGTYFSADQFLAEHPSSLRVGAVVLELQKQYDGVDFAALDSILSRGHRVIVYSHIAT
                     DEVILTALDRGAVTYLTKSESKEHLIDAIRAAGTDTPYVGPRMAMAMLNHSTVGRANL
                     APREREVLIAWFGTESKDLVAQQLQIAPTTVRTHLQRVRAKYAAVGRPATTKAALVAR
                     AIQDGIVNVDDI"
     gene            complement(4109..5224)
                     /gene="ald"
                     /locus_tag="VFC01_22685"
     CDS             complement(4109..5224)
                     /gene="ald"
                     /locus_tag="VFC01_22685"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZN82458.1"
                     /translation="MRVGIPTEIKNNEYRVAITPAGVAELVRRGHEVLIQAGAGEGSA
                     ITDADFKRAGAQLINSVDEVWAEAELLLKVKEPIEPEYSRLRKGQTLFTYLHLAASKP
                     CTDALLSSGTTSIAYETVQTADGALPLLAPMSEVAGRLSAQVGAYHLMRTQGGRGVLM
                     GGVPGVAPAEVVVIGGGMAGDNAAAVAWGMGAHVTVFDLNVNILRKIDAEYGGAIETR
                     YSSQLDLEDAVKQADLVIGAVLVPGAKAPKLVTNSTVAHMKSGAVLVDIAIDQGGCFE
                     DSHPTTHDDPTFAVHDTLFYCVANMPGAVPRTSTYALTNATMPYVLKLADKGWQAACQ
                     ADPALAKGLSTHDGALLSEQVATDLDLPFTDPADVLA"
     gene            5319..>5690
                     /locus_tag="VFC01_22690"
     CDS             5319..>5690
                     /locus_tag="VFC01_22690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZN82459.1"
                     /translation="MPEGLTKPLGARARPPKDVQAVAIDAVDRRILTALHADARMSNN
                     ALAEHVGIAPSTCHGRVRRLQELGVIRGFHADIDPAAIGLTLQAMISVSLQSNARGKI
                     RNFIQHIRTRPQVMDVYFLAGA"
BASE COUNT         1004 a         1817 c         1878 g          991 t
ORIGIN      
        1 cgatcgaccg gatcaacgcg atcgccaacc cgcagctgcc caagatcggg gagcgtttcc
       61 tgggcacggt cgtcaagacc accgatttcg gtgcgttcgt gtcgctgctc cccggacggg
      121 atggcctggt gcacatctcc aagctcggca ggggcaggcg catcgccaag gtcgaagacg
      181 tggtcaaggt cggcgacaag ctgcgcgtgg agatcgccga catcgacaac cgcggcaagg
      241 tctcgctgat cctggtcgcc gaagaagaca cagcggacgc gtcctccagc gcggatgcac
      301 ccgtcgatgc cgcaaccgcc agcagctagg tcccgggcgc tgcatcgcgg cgcccgcgcc
      361 gacaaaacgt cggaagcggt ccgccgcacc accttgcccg gtggcctccg tgtcgtcacc
      421 gagtacatcc cgtccgtgcg ttccgcatcg gtcggtgtgt gggtcggcgt cggctcgcgc
      481 gacgagggac gcagcgtggc cggtgcggcg catttcctgg aacacctgct gttcaagtcg
      541 acgccgacgc gcaccgcggt cgagatcgcc caggctgtcg atgccgtcgg cggggagctg
      601 aatgcgttta ccgccaggga gcacacctgt tactacgcgc atgtgctcga caccgacctg
      661 gagttggcca tcgacctggt cgccgatgtg gtgttgcgcg gccgttgcgc cacagatgat
      721 gtcgaagtcg aacgcgccgt ggtgctcgaa gaaattgcga tgcgggacga cgacccggag
      781 gacaccctgg gcgacgtgtt cctctcggcg atgttcggcg agcatccggt aggccgcccc
      841 gtgatcggcg gcgtcgactc gatttcggcg atgacacggg cacagctgca ttcgttccat
      901 atgcgccgat acacccccga gcggatggtg gtggcggtgg cgggcaacgt cgagcacgac
      961 gaggtggtcg cgctggtgcg ggaccacttc ggggcgaggc tgattcgcgg ccgccgaccg
     1021 ctggcaccgc gcacaggcac cggccgggtt gccggcaggc cgaccctgcg gatggtccac
     1081 cgcgacggtg aacagacaca cctgacactc ggtgtgcgca cccccggcag gcactgggat
     1141 cagcgctggg cgctgtcggt gctcaacacc gcgctgggcg gcggcctgag ttcgcggctg
     1201 ttccaagaga ttcgggagac gcgcgggctg gcgtactcgg tgtactcgac cgtcgacacg
     1261 ttctccgaca gtggcgcgct gtccatctac gccgcgtgcc agcccgagcg cttcgacgag
     1321 gtagtccggg tgaccaccga agtcctcgag aaggtggcac gtgacggcat caccgaagcg
     1381 gaatgccgca tcgccaaagg ctcactgcgc ggcgggctgg tgcttgggtt ggaggactcc
     1441 ggctcgcgga tgaaccgcat cggccgcagt gagctgaact acggcgaaca ccgcagcatc
     1501 gcgcataccc tcggcaaaat cgaaggcgtc accctcgacg aggtcaacgc ggtcgcgcat
     1561 caactcctta cccgacagtt cggcgccgct gtccttggcc cccagcggtc gaaaaagtct
     1621 ctgccacagc cgcttcggag gatcgcgggc tgaccggtag cctgggtcgg tgcctggact
     1681 gtctcggcgg caggcgctgc tcggcgggtt gaccctcgct gcagcggcgg cctgcgcgac
     1741 caaacccgta ctcgccgatc ctgaggagcc gccgccgatc ggggaacaca tcgcagcgct
     1801 tgagcgacgc cacgacgcgt acgtcggcgt gtatgccgtt gacctcgacg gtggtcggtc
     1861 catctcccac cgcggccagg aagcgttcgc catgtgctcg acgttcaagg gctacgcggc
     1921 ggcacgggtg ctgcagatgg tcgaacgcga cgagctgacc ttggatcaga gggtcttcgt
     1981 cgatccggcc gcgatcgtcg cgaactcgcc gaggactgcg ccccgggccg gtggtgacat
     2041 gacactcgac gagctatgcc aggcggcgct gcaggtgagc gacaacaccg cagggaacct
     2101 gctgctgaag accatcggcg ggccgtccgc tatcaccgcg ttcgcccgca gtatcggtga
     2161 tcccagctcg cggctggacc gctgggagac ggcgttgaat tctgcggttc ccggcgaccc
     2221 ccgcgacacc agcacacccg aagcactcgg ccgtggctat cgcgccctgc ttgccggcga
     2281 cgcgcttgcg cccgcgcagc gccagcagct cgaaacatgg atgcgggcga acgagacgtc
     2341 cagcatgcgg gccgggctgc cgccggactg gaccaccgcc gacaagaccg gcagcggcga
     2401 ttacggcacc accaacgacg ctggcatcgc gtacggaccc gcaggtcagc gtgtgctgct
     2461 cgcgatgatg acgcgctcgc agggcaacga cccgaaggcg cagaatctgc ggcccgtcat
     2521 cggcgagctc agcgcgatgc tcttacccga agtgctggcc taggtgattt tctagggaaa
     2581 cacccgattc cttgtcggct ctcgtgtcat acgtttgctc cacagaggag cagcgccagc
     2641 atagtggcct gatcggggac ggagcgagag accgtggcga tcgagacgat agcgcttcat
     2701 gactacctgc caccggagga gcccaatggc gcagtcgcgg ttatcgatga acacgaggcc
     2761 atccacgcgg cagtagagct gtggtgcaac caggcgcaga tcgggttcgc gggcacctac
     2821 ttctcggccg accagtttct cgccgagcat ccttcgtcgc ttcgcgtcgg cgcagtcgtg
     2881 cttgaactgc aaaagcagta cgacggtgtg gacttcgccg cgctcgacag catcctgtcg
     2941 cgagggcacc gggtaatcgt gtattcgcac atcgcaactg acgaagtgat cttgacggcg
     3001 ttggaccgcg gagcggtcac atacctgacg aaatctgaga gcaaggaaca tttgatcgac
     3061 gcgatccgtg cagcgggaac cgacacgcct tatgtgggtc cgcggatggc gatggcgatg
     3121 ctcaaccaca gcacggtcgg tcgcgcaaac ctggctccgc gggaaagaga agtgttgatc
     3181 gcgtggtttg gcaccgaaag caaggacctc gtcgcccaac agctgcagat agcgccgacg
     3241 acggtgcgca cgcacctcca gcgtgtgcgg gccaagtacg ccgcggtagg gcggcctgcc
     3301 accactaagg ccgcgttggt ggcccgagcc attcaggacg gcatcgtcaa cgtcgacgat
     3361 atttagcgcc cgttgctccg atagttgcca tcgcaaattt caagagttgc cataaaccgg
     3421 gaccaccgtg cgaacactcg gacgtggcga gtaatttctc gcctcagtcc ggctgtgtcg
     3481 tctgcttact ctcttccccc cggagagtgc ggcggcccag cgtggttcgc acggtggagt
     3541 ctctcagtac gtgcacagaa tctgaacttg agcaacatca tggtgcttcg gcgaccacgt
     3601 cgcccgacaa cccctcgtgg ccgggcggca ggcctacagc tgcatttgtc tcagcacttc
     3661 tgcgtcagcc cgccgagatg atcagttagt tctcctttcc ccttgttatg gaactttgtc
     3721 cccggtagtg tccttgcgtg ctctgagcgt atgcctaatt gcggcgattg acacgagtac
     3781 tgctctactt aatctgtgat cgtgtcgaat ttcgaaatcc cacatcgtta gcaaatgtcg
     3841 cacgggtcgt ccgaacagac gacggcgagg tcagcgggcc gtttcgacag cgggcgagtg
     3901 ccgggataaa tgcgaagagc cactgcccac gaatgggaat cgaatcccga aaatgggaca
     3961 gtccggccaa ttctgcaaga actttgctat cgatgagtgt gacaatttgc taagcgcgct
     4021 tctcggcccg gtttgttgcg gtttcccatc gcattgcaaa acccgggcac cgctcaggtg
     4081 cggcgcccgg gttcgcgggt gtgaagcgtc aggccagcac gtcggccggg tcggtgaagg
     4141 gaaggtcgag gtcggttgcc acctgctcgg agagcagcgc gccgtcgtgc gtggacaggc
     4201 ccttggcgag cgccgggtcc gcctggcagg cggcttgcca gcccttgtcg gccagtttca
     4261 gtacgtaggg catggtggca ttggtcaacg cgtacgtcga cgtgcggggc actgcgccgg
     4321 gcatgttggc cacgcagtag aacagcgtgt cgtgcacggc gaacgtcgga tcgtcgtggg
     4381 tggtggggtg cgaatcctca aagcagccac cctggtcgat ggcgatgtcg accagaaccg
     4441 cgcctgactt catatgcgcg acagtggaat tcgtgaccag cttgggcgcc ttggcgccgg
     4501 gcaccagcac cgcgccgatg accaggtcgg cctgcttgac ggcgtcctcc aggtcgagct
     4561 gtgaggagta gcgcgtctcg atggcaccgc cgtactcggc gtcgatcttg cgcagaatgt
     4621 tcacgttgag gtcgaagacg gtgacgtgcg cgcccatacc ccaggcgacg gcggcggcgt
     4681 tgtcgccggc catgccgcca ccgatcacca cgacctcggc cggagcgacg ccggggacgc
     4741 cgcccatgag cacgccgcga ccaccctggg tacgcatcag gtggtaggca ccgacctggg
     4801 cggacaaccg gccggcgacc tcgctcatcg gggccagcag cggcagcgcg ccgtcggcgg
     4861 tctgcacggt ttcgtaggcg atcgacgtgg tgcccgagga cagcagggca tcggtgcacg
     4921 gcttggacgc cgcgaggtgc aagtaggtga agagcgtctg gcccttgcgc agtcgcgagt
     4981 actcgggctc gatcggctcc ttgaccttca gcagcagttc ggcctcggcc cacacctcgt
     5041 cgacgctgtt gatcagctgg gcgcctgccc gcttgaaatc ggcatcggtg atcgccgaac
     5101 cctcgcctgc accggcctgg atgagcacct cgtggccgcg gcggaccagc tcggcgacac
     5161 ccgcgggggt gatggcgact cggtattcgt tgttcttgat ctcggtcggg attccgacgc
     5221 gcatgacggc tcctaaaagt cgtgttggct acctcagatt gtgaagaact ttcgctgtaa
     5281 tggcaatcac cccgatgaaa cttcgatagc atcgtgttat gccagaagga ttgacgaaac
     5341 cgcttggggc aagggcccgg ccgccgaagg atgttcaggc agtcgcgatc gacgccgtcg
     5401 accggcgcat tctgaccgcc ctgcatgccg acgcgcgcat gtcgaacaac gcgctcgccg
     5461 agcacgtcgg catcgccccg tcgacatgtc acggccgcgt ccggcggttg caggaactcg
     5521 gggtgatccg cggtttccac gctgatatcg atcccgcggc catcgggctg acgctgcagg
     5581 cgatgatctc agtcagcctg caatccaatg ctcgcggcaa gattcgcaat ttcattcagc
     5641 acatccggac ccggccacag gtgatggacg tttacttcct cgccggagcc
//
LOCUS       DASRJA010000520         6061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2791883,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000520 DASRJA010000000
VERSION     DASRJA010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2791883"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..443
                     /locus_tag="VFC01_22695"
     CDS             141..443
                     /locus_tag="VFC01_22695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82460.1"
                     /translation="MTAIDWPTGATEVSDWADVGHPDEFRSFLGPKWTLPGIRWVWGD
                     NDEDGFVTVSGTQLRDGTVEGRCPWPTQLPKAVPTPSAATSPPTTGSHSTRSTRTS"
     gene            complement(515..742)
                     /locus_tag="VFC01_22700"
     CDS             complement(515..742)
                     /locus_tag="VFC01_22700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82461.1"
                     /translation="MSTSRRCVHSSRLNGTASSSSDADVAPHSASDKIDLARANLKDT
                     TLRPRSTTLRKSSEAVVADDMFVTLLNIGDN"
     gene            830..1369
                     /locus_tag="VFC01_22705"
     CDS             830..1369
                     /locus_tag="VFC01_22705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82462.1"
                     /translation="MQPSTSGEPSDHEERTRQTALLVDRLHAIVEELELLHPGRKFPM
                     DGHLVGSLAEAAAEAIFDITLKPPSNMGYDAISEPDHRAVEIKGTYGTGGVAIRPTSH
                     AHASALIVLRLSRKSGEPHEIVYNGPFAVAAAAAGPVGSNGQARIGLNRLRVLNKSVD
                     DVDRVRRRDLEASTALDVE"
     gene            1430..2035
                     /locus_tag="VFC01_22710"
     CDS             1430..2035
                     /locus_tag="VFC01_22710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HZN82463.1"
                     /translation="MRVDGRWWVRFVHYGPGATAVAVATLLALSTTTANADPVADPIG
                     PACAYTTGGRAGAGSVADAAQQPLAVAVSRIRTLSALSAALSGKLNPAVNLVDTLNGG
                     QITAFAPVDNAFSRLPPEATASLHIDAVRLTQLLTYHVIPGRFSPTNIDGIHKTLQGG
                     DLSVTGAADHLWVNDALVLCGGITTANATLYLIDTVLTPTS"
     gene            complement(2126..2386)
                     /locus_tag="VFC01_22715"
     CDS             complement(2126..2386)
                     /locus_tag="VFC01_22715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82464.1"
                     /translation="MKRRMKGASGTAGDTGDSSVAVKVRVRVYPGTDGEVGGTVVEDF
                     GEMAAQAVDIGGEHFADPARRWAVQLDTGDLVFVNTDQLVAE"
     gene            2658..3554
                     /locus_tag="VFC01_22720"
     CDS             2658..3554
                     /locus_tag="VFC01_22720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZN82465.1"
                     /translation="MYLITGAAWASGRVSDSVVELLRGNGEPVRVMVRRDDGRANRFR
                     ELGAEVVVADLTSPVDVVEAMRDVTRMFFNTHISSDYLQQVAIACAAARERDGLEVIV
                     NMSQMTVAQMTLTSSEESRQQQLFWLAEQIMNWSGVPAVHVRPTMLLDNPLFTVLARR
                     SVREHDVLALPFGTGRTSPIAAEDVARVVAALLLTPPKPGQVYGLTGPQVLDITGLAE
                     QYSRALGRTIRAEDIPYDDWLRQYLRKSGLSDLVQQHLATVVRLHRENRYDRSTHDVE
                     QVTGQPPQTVEHYVASHPDLFS"
     gene            3606..3764
                     /locus_tag="VFC01_22725"
                     /pseudo
     CDS             3606..3764
                     /locus_tag="VFC01_22725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005110344.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3859..4254
                     /locus_tag="VFC01_22730"
     CDS             3859..4254
                     /locus_tag="VFC01_22730"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN82466.1"
                     /translation="MKVLKWAVVLLTTVLGLMAGASTLATAAPDTQADATYLHLLDQN
                     GVSYPNPEAMVSFAHDLVDQLSAPPQTDASVGSIVSTGEERISQHDMGVIIAAAVVAY
                     RPSLIPVVRHWADSAAGFPCGSAALGVLR"
     gene            5081..5815
                     /locus_tag="VFC01_22735"
     CDS             5081..5815
                     /locus_tag="VFC01_22735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HZN82467.1"
                     /translation="MIIEPAKTFSRMFRGYDPAAVDAYIEALTTKQQFLLNDVESLRA
                     RLKERDDDAAALRKEVALLRDTSPSPHAMQHRMAKMLQRAVDEISEMQAEARAEAEAL
                     IAATKAEAEAEQRKHQELLADMAAKRNALDAEYEETKKKLDAELARMREETQSEIDEA
                     WQDAQLEREQLLADAKQEGDYYREQARRAVDQASQQRIKVLEQLMGVYRDLEAVPATL
                     ESAYQEAKDSPEASVAVPLDQKISAG"
BASE COUNT         1168 a         1954 c         1890 g         1049 t
ORIGIN      
        1 atgctcgatg agatcaaaga gcccgtcgag cccggcggcg gcgtgtaccg cgaccacttc
       61 gcgatcagcg cggacggggc acgccgtctc gcacacgcgc tgcttgagac ggctgacgag
      121 atcgacgggt gggtgaaccg atgaccgcga tcgactggcc aaccggcgcc actgaggtct
      181 ccgattgggc cgacgtcgga caccccgacg agttccgcag cttcctaggt ccgaaatgga
      241 cactgcccgg aattcgctgg gtctggggcg acaacgatga ggacggcttc gtcaccgtct
      301 ccggaacaca gctccgcgac ggcaccgtcg aaggccgctg cccttggccg acccaattgc
      361 cgaaagccgt tccgacaccc agcgcggcga ctagcccccc gactacggga agccacagca
      421 ccaggtccac ccggacaagt tagctcagag gctgtacacg gcgggtgctt cgccgcttcc
      481 tcatactgcc cgaggcgttg tccccgcagg gcgcttaatt gtcgccgatg ttgagtagcg
      541 tcacgaacat gtcgtcggcg accactgctt cggaggactt tcgcagcgtc gtgctgcggg
      601 gtcgcaacgt ggtgtccttc aaattcgctc tggccaagtc gatcttgtcg ctcgccgagt
      661 ggggtgcgac atcggcgtcg ctggaagaac tcgcagttcc gttcagccgc gagctgtgca
      721 cacatcgccg ggaggtggac acgcaggcga ccagggcagg gagtcggttc ctagacgcct
      781 gccgtcacgc catagcgcaa gagcaacgaa ctgctaggga aggccgctaa tgcagccatc
      841 aaccagtggc gagccgtctg atcatgagga gcgtacccgc cagacagcgc tattggtcga
      901 tcgactgcat gccatcgttg aagagcttga gcttttgcat ccgggtcgga aatttccgat
      961 ggatggacac ctagtcggat cgctagctga agccgctgcc gaggccatct tcgacatcac
     1021 gctcaagcca ccgtcgaaca tgggatatga cgcaatcagc gagccagatc atcgtgccgt
     1081 cgagatcaaa ggcacctacg gtacgggcgg cgtggcgatc aggccgacgt ctcacgctca
     1141 tgcctcggcg ctgatagtgc ttcgtttgtc ccgaaagtct ggcgagccgc acgagattgt
     1201 ctacaacggt ccgtttgctg tggctgccgc tgctgccgga ccggtcggct ccaatggtca
     1261 agctcggatc ggcctgaatc gactacgtgt gctgaacaag tccgtggatg acgtggaccg
     1321 cgtacgccgc cgcgacctcg aagcgtccac agccctcgat gtcgaatagg tcttgctggc
     1381 gcacctgggg tcagagttaa cttcgccgcc tccttggcgg aaaatgaaca tgcgcgtcga
     1441 cgggaggtgg tgggtgaggt tcgtgcacta cgggccgggc gcgacagccg tggccgtcgc
     1501 gacactgctg gcgttgtcga caacgaccgc caacgccgat ccggtggctg atcccatcgg
     1561 cccagcctgt gcctacacaa cggggggccg agcgggggcg ggctcagttg ccgatgcggc
     1621 acagcagccg ctggccgttg ccgtttcgcg tatccgcaca ctcagcgcac taagcgcggc
     1681 actgtccggc aagctcaatc ccgccgtgaa cctggtcgat acgcttaacg ggggccagat
     1741 caccgccttc gcgccggtgg ataacgcgtt ctcccgactg ccccccgagg caaccgcctc
     1801 gctgcacatc gacgctgtgc ggctgactca gctgctgacc tatcacgtga taccagggcg
     1861 gttcagccct accaacatcg acggtatcca caagaccctg cagggcggcg acctatccgt
     1921 cacgggtgca gctgatcacc tctgggtcaa cgacgcactg gtgctctgcg gcgggatcac
     1981 caccgccaac gccacgctct acctcatcga caccgtactg acccccacca gttgatgatc
     2041 aaccgtgctg tcgtggcgct agcaacccgg catcagtgtc agccaacccc acttgctgac
     2101 cgcgccgatc gatccgacat accgatcact cggcaacgag ttggtcggtg tttacaaaca
     2161 ccagatcgcc ggtgtccaac tgcaccgccc accgtcgggc cgggtcagcg aaatgctcgc
     2221 cgccgatatc gacggcttgg gccgccatct caccgaaatc ctcgaccaca gtgccgccga
     2281 cctcgccgtc cgtccccgga tatacacgca cccgcacctt caccgccacg gagctatcgc
     2341 cggtatcgcc agccgtcccg ctagcgcctt tcatccgtct tttcatccgt catcacacta
     2401 gggcgtgcgg gtgaagatac tggcgcgatt agagcacgac cagctaacga gcctccgcct
     2461 attggcatcg accatccgcg cctttgtctt ttcatcgatg tggctgggcc agtgccgaaa
     2521 tgggggcgag aaatctttag tcagatttct gttcgggaca gcggcctggc acggggtcta
     2581 cgacggcgat cggccaatgg ggcctacgtc ggtcgactgt agggagtcgt cagccgtccg
     2641 cggctaaatt gacggctatg tatctgatca ccggcgcggc ttgggcttcg ggcagggtca
     2701 gcgactcggt tgtggagctg ctgcggggca acggggaacc ggtccgggtg atggtgcgcc
     2761 gcgacgatgg ccgcgcgaat cggtttcgcg aactgggagc cgaggtcgtg gttgctgatc
     2821 tcaccagtcc ggtcgacgtt gtcgaagcga tgcgagatgt cacgcggatg ttcttcaaca
     2881 ctcacatctc gtcggactac ctgcagcagg tcgcgatcgc ctgcgcggcc gcccgtgagc
     2941 gggacggcct ggaggtgatc gtcaacatgt cgcagatgac ggtcgcgcaa atgaccctca
     3001 ccagcagcga ggagtcccgc cagcagcagc tgttctggct cgccgagcag atcatgaact
     3061 ggtcgggtgt accggcggtt cacgtgcgtc ccacgatgtt attggacaat ccgctgttca
     3121 cggtgttggc caggcgatcg gtacgcgagc acgatgtgct ggccttgccc ttcggcaccg
     3181 ggcgcacctc gccgatcgcc gccgaagacg tggcgcgggt ggtcgcggcc ctgctgctca
     3241 ccccgccgaa gccggggcag gtctacgggc tgaccggtcc gcaggtcctc gacatcaccg
     3301 gcctagccga gcagtactcg cgcgcgctgg gacggacgat ccgcgcggaa gacattccgt
     3361 acgacgattg gctgcgccag tatctgcgga aatcggggct gtccgacctc gttcagcaac
     3421 atctcgcgac cgtggtgcgt ctgcatcgcg agaaccgcta cgaccgctcc actcatgacg
     3481 tcgaacaggt caccggtcaa cccccacaga ccgtcgagca ctacgtcgcc tcgcacccgg
     3541 acctgttttc ctaaatccgc cgccgtcggg actcttgtac ctgctcgcga agctcggagg
     3601 gcaccgggca gccgattggc cgtccgaaag cgcttgacga gaaggaagcg gctctggcgc
     3661 agcggatgca tgccagtggt gaatcggcca gcaccatagc cgccgcagct ctcggcgtta
     3721 gaagagaaac ggtttaccgg gtactagccg aagacgcgga gtgagtcgct gacgacgctg
     3781 gacaccacaa aggtttggaa ctcacgatgg aggcggggtt gtcctccagg tcgtgccgcg
     3841 aacgtgaagg agaacatgat gaaggttctg aaatgggcgg tagtgctgtt gacgacggta
     3901 ctgggcctga tggccggtgc ttcgacgctg gcgacagcgg cccccgacac ccaagccgac
     3961 gcgacctatc tgcatttgct cgaccagaac ggggtgtcct accccaatcc cgaggcgatg
     4021 gtctctttcg ctcatgacct cgtggaccaa ctgagcgccc ccccgcagac cgacgccagt
     4081 gtgggctcga tagtcagtac cggcgaggag cggatttctc agcacgacat gggcgtgatc
     4141 atcgccgccg cagtggtcgc ctacagaccg tccctgatcc cggtggttcg gcactgggcg
     4201 gacagcgccg ctggcttccc gtgtggttcc gcagctctgg gggtgctgcg atgaagaaac
     4261 tgatgacagc tgtgttggcg gtctgcccca aggtccgggg ctcggcctca gagctgcgcc
     4321 aggttgacgt gcggagcagg caccgggctc agtgcccgcc gcagccggac aagaggaacg
     4381 gggcaggagc cgaggcaccc gagggggaca tccccgcgtg agatccccct ggttaacacg
     4441 gatgcgcggc acctgcctcc aaacatcaag tcgcgcaacg tgttgccagc gcaacccgca
     4501 ttgcctcccc gtcggcttcc caccgacgag tcgacccctc aatatcggtg aacagaaaac
     4561 gtcaccaccc ccgacggagc agccgccgtc atggcatcgc ctcggcccac ttcatgtgcc
     4621 cctcgaagcc aaacggatgt tgctatcgcc cggtatctat cgcgatagtc gcggtaggcg
     4681 gcttcgtcac caaccgcccg cgcgcgcagc gcctttaacc gcgcgatgaa gtcactcgtc
     4741 gcgaagccgc cgttggccac cgtcaccgcc accacgcggt cgatcgcggt ctcggcttcg
     4801 gccagttcat cctcggagcc gcgcggtaac cagccgcccg gaagcgcgat tgtcagggtc
     4861 taccgccact cttcgcgcat cgacggccac gatcccccca catagaatcg acgccgacaa
     4921 ggccgatgtt ttctcggtac ccgacttttc gttctaggca cttttggact gctctgcgcg
     4981 agcatcggtc acgtggtcgg gtcgtcgcat gaccgtgaaa tactgactcg ggggtcgacc
     5041 tcacagggtc taccaggcgt ttccagaagg ggaaattgca gtgataatcg aacccgcaaa
     5101 gacgttctct cgcatgttca ggggctacga tccggctgcg gtggatgcct acatcgaggc
     5161 gttgaccacc aagcagcagt tcctgctcaa cgacgtcgag agcctcaggg cccggctgaa
     5221 ggaacgcgac gatgatgcag ccgcgctgcg gaaggaggtc gccctcctaa gggacacctc
     5281 accctcgccc cacgcgatgc aacatcggat ggcgaagatg ctgcagcgtg cggtcgacga
     5341 gatttccgaa atgcaggccg aggcgcgggc cgaggcagag gcgctgatcg cggcgaccaa
     5401 ggccgaagcc gaggccgagc agcgaaagca ccaagagctt ttggcggaca tggccgcaaa
     5461 gcgaaacgcc ctagacgccg agtatgagga aaccaagaaa aagctcgacg ccgaattggc
     5521 caggatgcgc gaagagaccc aatcggagat cgatgaggcg tggcaggacg cccagctgga
     5581 gcgtgagcag ctcctcgccg acgcaaagca ggagggtgat tattatcgtg agcaggccag
     5641 gcgggcggtg gaccaggcga gtcagcaacg aatcaaggtc ctggagcaac tgatgggcgt
     5701 ataccgcgac ctagaagctg ttccggccac tcttgagtcg gcataccagg aagcaaagga
     5761 ttcgccggag gccagcgtcg cggtgccctt ggaccagaaa atcagcgccg ggtgagcccg
     5821 cgcggcaagc ctcgtagacg accactcacg caccagttga ctgatcaacc gtgtgtcgtg
     5881 gcgctcgacg cagacgttag cggtcggcga cgacagcaca tacgggtgag aatccctgac
     5941 cgtcccacac cacggcatca gtgtcagcca accccacttg ctgaccgcgc cgatcgatcc
     6001 gacataccga tcactcggca acgagttggt cggtgtttac aaacaccaga tcgccggtgt
     6061 c
//
LOCUS       DASRJA010000521         3612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3466219,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000521 DASRJA010000000
VERSION     DASRJA010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3466219"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VFC01_22740"
     CDS             complement(<1..1148)
                     /locus_tag="VFC01_22740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZN82468.1"
                     /translation="MLELLRPRLPRLLLAVALGVLSLGSALALSGVAAWLITRAWQMP
                     PVLDLSVAVVAVRALGISRGVLGYCQRLASHDTALRAAADTRERLYRRLADAPAEVAM
                     RLHSGELVARIGASIDELSDVLVRAVLPIAVAAVLSLAAVAAIAVISPAAALVLAICL
                     VIAGLVAPWLAARAATAAENVAVQHHSGRDVAVMLALEHAPELRVAGRLDDVIAEAEG
                     RHSAWGRATDRAAAPAALAAAAPTAAVGISVIGAVVAAIGIADAVAPMTLAILMLLPL
                     SAFEATTALPAAAVQLTRSRIAARLLRDLTEPDNDTRIRPQVPPIALESGARLAITGP
                     SGAGKTTLLMAMAAAPKDASKAVFFAEDAHLFATTVRDNLLVARGDAT"
     gene            complement(1141..2619)
                     /locus_tag="VFC01_22745"
     CDS             complement(1141..2619)
                     /locus_tag="VFC01_22745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZN82469.1"
                     /translation="MRRFLTASVVCGVVISGTTIVSAVVLAHVIAGVITDPATRTVAH
                     WAGPLWILVALWVIRTFAHWLQGRLSQRGATAVIVGLAGEVLRTVTALPPRQLAAHRD
                     SAAAMITRGLDGLRPYFTAYLPSLLLAGILTPAAVVVIAVYDWESAVIVLIALPLIPI
                     FMVLIGLLTRERSAASLAAMTTLQSRLLDLVAGIPTLRALGRVGGSVQRITELGAAHR
                     RSAMSTLRIAFLSALVLELLATLGVALVAVGVGLRLVYGDMTLAAGLTALLLAPEVFW
                     PLRRVGMEFHAAQDGKTAADKAFQLLDTLQNQPGGTPALDVDLEPGVVTVLTGPNGVG
                     KTTLLQSILGLDSPRPQEWWSQVAWLAQRPVLVPGSIRDNLELFGPLVDLEGAISAAG
                     FDEVLAVLPDGLETVIGRDGVGLSLGQRQRLGLARVFGSPASVLLLDEPTSHLDAQME
                     ARVLQAIVARAGAGAAVLVVGHRDPVLAIGDRVLHMGSLVNA"
     gene            complement(2643..3611)
                     /gene="cydB"
                     /locus_tag="VFC01_22750"
     CDS             complement(2643..3611)
                     /gene="cydB"
                     /locus_tag="VFC01_22750"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZN82470.1"
                     /translation="MLEGFDFGVGMLMIPLGDTENRRRAVLNTIGPVWDANEVWLITA
                     GASMFAAFPNWYATMFSALYLPLLAILFAMILRIVGIEWRGKIDDPKWRARADAGIAV
                     GSWVPAILWGVAFAILLRGLPIDANHRVDLSIGDVLNAYTLLGGLATASLFAFYGAVF
                     IALKTGGPVHDDAFRFAKVLSVPVIVLAGGFGLWTQLAYGKAWTWLALGVAVVALLTA
                     VALVWARRNEGRAFVSMVVVIAAVAVLVFGAMYPNLLPSTLNPEWSVTIYNGSSTPYT
                     LKIMSWASLTLLPLVMIYQGWTYWVFRRRITADAIPASIGLARRSN"
BASE COUNT          694 a         1251 c         1181 g          486 t
ORIGIN      
        1 tcggtggcgt caccgcgggc aaccagcaga ttgtcacgca cagtggtggc gaacagatgc
       61 gcgtcttcag cgaaaaacac cgccttcgat gcgtctttgg gggctgcggc catcgccatc
      121 aacagcgtgg tcttgccggc accgctgggg ccggtgattg caagccgggc acccgattcc
      181 agtgctatcg gtgggacctg cggccgaatt ctcgtgtcgt tgtccggttc ggtcaggtcg
      241 cgcaataggc gcgctgcgat tcgtgagcgg gtgagctgca ccgcggcggc aggcaatgca
      301 gtggtcgcct cgaacgcgga cagcggcaac aacatcagaa tcgccagggt catcggtgcg
      361 accgcgtcgg cgatcccgat cgcggcgacg acagcgccga tcacgctgat gccgacagcc
      421 gcggtcggtg cggcagcggc aagcgccgcg ggcgcggctg cccggtcggt ggcgcggccc
      481 caggcgctgt gcctgccttc ggcttcggcg atgacatcat cgagccggcc cgcgacccgc
      541 agttcggggg cgtgctcgag ggccagcatc accgcgacgt cgcggcccga atgatgctgc
      601 accgcaacgt tctccgcggc ggtcgcggcg cgggcggcca accacggcgc caccagcccc
      661 gcaatcacca gacagatcgc gagcacgagc gccgcggcgg gggagatcac cgcgatggcg
      721 gcgacggcgg ccagtgacag cactgcggcg accgcaatcg gcaacacggc gcgcaccagc
      781 acatcggaca gctcgtcgat cgaagcgccg atgcgcgcca ccaattcacc actgtgcaac
      841 cgcattgcga cctcggcagg cgcatcggca agccggcgat agagccgctc acgtgtatcg
      901 gcggccgccc gtagcgccgt gtcgtgcgac gccagccgct ggcagtatcc gagcacaccg
      961 cgcgagatgc cgagtgcgcg caccgccacc actgccaccg aaaggtccag cacgggaggc
     1021 atctgccagg cccgggtgat cagccatgcg gcgacaccgg aaagcgccag cgcgctgccc
     1081 agtgacagca cgcccagtgc cacggccagc aatagccgtg gcaaccgtgg gcgcagcaac
     1141 tcaagcattg accaggctcc ccatgtgcag tacccggtcg ccgatcgcca gtaccgggtc
     1201 gcggtggccg acgacgagca cggccgcccc ggcacctgcc cgcgcgacaa tcgcttgcag
     1261 cacacgggct tccatctgcg catccaggtg cgacgtgggc tcgtcaagca gcagaaccga
     1321 tgcaggcgag ccgaataccc gcgccaggcc gaggcgctgg cgctgtccca gtgacaagcc
     1381 gacgccatcg cgtccgatga cggtctccag cccgtcgggc agcacggcaa gcacctcgtc
     1441 gaaacccgct gcactgatgg caccctcgag gtcgaccaat ggcccgaaca gttcgaggtt
     1501 gtcacggatg ctgcccggca ccaggaccgg acgctgggcc agccaggcca cctgagacca
     1561 ccactcctgc ggccgcggcg aatcgagccc gagaatgctc tgtagcagcg tggttttgcc
     1621 gacgccgttg ggtccggtca gcaccgtcac cacgcccggt tcgaggtcga cgtcaagcgc
     1681 aggcgtcccg cccggttggt tctgtagcgt gtcgagcaac tggaatgcct tgtccgctgc
     1741 agtctttcca tcctgtgccg catgaaattc catgccgact cggcgcagcg gccagaacac
     1801 ctccggcgcc agcagcaggg cggtcaggcc ggccgccagc gtcatgtcgc cgtacaccag
     1861 gcgcaggccg acgccgaccg cgacgagggc gacaccgagg gtggccagca gttcaaggac
     1921 gagcgccgac agaaacgcga tgcgcagggt ggacatcgcc gagcggcggt gggcggcacc
     1981 aagttcggtg atccgctgaa cggagccacc cacccggccc agagcacgca gcgtgggaat
     2041 gcccgcgacg agatcgagca gccgggattg cagcgtggtc atcgcggcca gtgatgcggc
     2101 cgatcgctcg cgggtgagca acccgatcag caccatgaag atcgggatga gcggcaacgc
     2161 gatcagcact atgacggccg actcccagtc gtagaccgcg atgaccacca ccgcggccgg
     2221 ggtgaggatg ccggcaagga gcagcgacgg cagatacgcg gtgaaatacg gtcgcagccc
     2281 atcgagaccg cgcgtgatca ttgcggcggc cgaatcgcgg tgcgcggcga gctgacgcgg
     2341 cggcagcgcg gtgaccgtgc gcagcacctc gccggccaga ccgacgatca ccgccgttgc
     2401 gccccgctgt gacagcctgc cctgcagcca atgcgcgaat gtacggatca cccatagcgc
     2461 caccaggatc cacagcgggc cggcccagtg cgcgaccgtg cgggtggcgg gatcggtgat
     2521 tactccggcg atgacgtgcg ccaacaccac ggcggacacg atcgtggtac cgctgatcac
     2581 cacaccgcac accaccgacg cggtcaggaa gcgccgcatc gccggggaag ccagcagtgg
     2641 gttcagttcg atcgcctcgc caatccgatg gatgcgggta tcgcgtcggc ggtgatccgt
     2701 ctgcgaaaca cccagtacgt ccacccctga tagatcatca cgagcgggag caaggtcagc
     2761 gaagcccacg acatgatctt caacgtgtac ggcgtggatg atccgttgta gatcgtcacg
     2821 ctccactccg gattgagcgt ggagggcaac aagtttggat acatcgcgcc gaacaccagc
     2881 acggcgacgg ccgcgatcac cacgaccatc gacacgaagg cccggccctc gtttcgccga
     2941 gcccacacca gtgcgacggc ggtcaacagg gcgacgaccg cgacgccaag cgccagccag
     3001 gtccatgcct tgccgtacgc gagttgcgtc cacaacccga atccgcccgc cagcacaatc
     3061 accggcaccg acaacacctt tgcgaaacgg aatgcgtcgt cgtgcacggg accgccggtc
     3121 ttcaacgcga tgaacaccgc gccgtagaag gcgaacagcg acgcggtggc aagtccgccg
     3181 agcagcgtgt acgcgttgag cacgtcaccg atcgacaggt ccacgcggtg gttggcgtcg
     3241 atcggcaagc cgcgcagcag aattgcgaac gcgacgcccc acaggatcgc cggcacccat
     3301 gacccgactg cgatgcccgc atcggcccgt gcccgccact tcgggtcgtc gatcttgccg
     3361 cgccattcga tgcccacgat gcgcaggatc atcgcgaaca ggatcgccag cagtggcagg
     3421 tacagggccg aaaacatcgt cgcgtaccag ttggggaacg ccgcgaacat cgatgcaccc
     3481 gccgtgatca gccacacctc gttggcatcc cataccggcc cgatggtgtt cagtactgcg
     3541 cgcctgcgat tttcggtatc gcccaaggga atcatcagca tgccgacgcc gaagtcgaaa
     3601 ccctcgagga tc
//
LOCUS       DASRJA010000522        10591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_290953,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000522 DASRJA010000000
VERSION     DASRJA010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290953"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFC01_22755"
     CDS             <1..344
                     /locus_tag="VFC01_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HZN82471.1"
                     /translation="PFEGNELLLLTTTGAKSGEPRVSPLSCKRIDGKLLIIAGNGGAE
                     TNPAWVYNLRANPRAHVELGTESFDVTARELPPAERDDIVPKLTAEVSAFAEFQAKTR
                     RVIPIFELQPA"
     gene            365..1243
                     /locus_tag="VFC01_22760"
     CDS             365..1243
                     /locus_tag="VFC01_22760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN82472.1"
                     /translation="MLPSRETAMTGEHNARGLVEFAKTAEEAGFDSVWVGDSLLARTR
                     AEPLSVLSAVAAVTDRVVLGIAALIAPLRHPLLAAAQAATVDQLSGGRLILGLGSGAP
                     LPESQREFDAVGMPFAGRGARLDKTVTLWRETWAGQGEFADALPTAGPDGPPVWLAGG
                     DTTKVIERVAATYDGWLPYLPDVNAYARAWDRIRAQAVREVTPALYATVNLSGERADD
                     ELDEYLRAYYGQPLEVMRYVQAIRGGSVNECVDWLARYVDAGAQHLILRIGSLTARPE
                     SVAERLLPALKELASP"
     gene            1240..1671
                     /locus_tag="VFC01_22765"
     CDS             1240..1671
                     /locus_tag="VFC01_22765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN82473.1"
                     /translation="MTEIPDAETMKAFNKNIVDEFRANGGKVGGPFEGATLLLLTSTG
                     AKSGQPRLAPLAYLTIDGKTIIIGSKAGADTNPDWVHNLRANPRAHIEVGADGYDVIA
                     RELPPEERDATYPKVVALAPGFGEYQSKTSRVIPLFELQRT"
     gene            complement(1805..2812)
                     /locus_tag="VFC01_22770"
     CDS             complement(1805..2812)
                     /locus_tag="VFC01_22770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HZN82474.1"
                     /translation="MRRRAWVVAGAIVYAALAGCSTSSQGGGGLPLRQISETALTGGP
                     VRFDYTALDVERGRLFIAHMGAGELIEVDVHAHGVVRTVPDLPDVHGVIVVPGKHRVY
                     ATATGNNQLAAIDADSGQVVFRAPTDTYPDGLAYDPVRRTVWTTNESAGSETVIDADT
                     GAVRATVPLGGEVGNVVYDNFLDQMVIAVQGRNELAVIDPVSFTVTERIPTPGCDHPH
                     GQALDVTDQVMFVGCEANATMVTVDLSSRTVVDHHGVGETPDVLAYDAGAGRVYVATE
                     SGWVSIFEHQHGHLTERGSAHLADGAHSLALDPTTHHTYFPIPKGPKGSPVLWEFEPT
                     N"
     gene            3019..3765
                     /locus_tag="VFC01_22775"
     CDS             3019..3765
                     /locus_tag="VFC01_22775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN82475.1"
                     /translation="MLSSQQALSPQLVVVCVAMVIAAALVYWLTALGSPWTVPWAAVR
                     AAMQLAAVAGVLAAALARLWSSILVLAVMFVVAGLTAARRSQASRGSPLLSVSLALGM
                     VAVLPLLLFSGVVPLTGVAVVPIVAIVLGNTMTAVAVAARLALDVLQLRAGEVEAALS
                     LGMSERDSRMEIIGQTAANALLPNLDSTRTVGLVTLPGAFVGVLLSTGSAAQAAAVQI
                     LVLIALLLCQTCGVAATIELIARGMIARQR"
     gene            complement(3880..5061)
                     /locus_tag="VFC01_22780"
     CDS             complement(3880..5061)
                     /locus_tag="VFC01_22780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZN82476.1"
                     /translation="MAGYAGRDAVIVGAVRTPIGKGKAGGALHDVLPVDLLAHSLKEL
                     VNRTGIDPAQIDDVIAGAVTQVGDQAVNIARNAVLGAGFPESVPGTTVDRQCGSSQQA
                     ISFAAQGVISGAYDIVIAAGVESMGRVPMGSSVLPGSDPFGAMAQRYPEGLVPQGISA
                     ELIAAKWGFSRQQLDEFSAGSHEKAAAATKEGRFDNELIPIAGLTTDEIIRPGTTVDT
                     LAGLKPAFYTPAYEARFPQIKWEITPGNSSPLSDGSAAVLITTSEVAARLGLRPLARI
                     HTATVVGSDPLYMLTGVIPATEKVLQRAGLTLSDIDLFEVNEAFAPVVLAWAHDVGAD
                     LAKTNVNGGAIAIGHPLGASGARIMTTMVNALEQRGGRYGLQTMCEAGGMANATIIER
                     L"
     gene            5156..5626
                     /locus_tag="VFC01_22785"
     CDS             5156..5626
                     /locus_tag="VFC01_22785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82477.1"
                     /translation="MTVLQGQLADRDAWSAVGKCPIEKTMALLGTKSAMLIMREAYYG
                     TTRFEDFWRRIGVTKAAASARLNELVEAGLLQRRPYKEPGQRSRDEYVLSDAGLDFMP
                     VIWAMFEWGRRHLPYSPQLRLAHRDCGAEATVEIRCEKGHHVPADELVVQLAQS"
     gene            5689..6147
                     /locus_tag="VFC01_22790"
     CDS             5689..6147
                     /locus_tag="VFC01_22790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HZN82478.1"
                     /translation="MSNTTTAHPVHHGFVPPKLPKPINPALAKHYEARAASIQNRIAD
                     AITRFAGSMAFVYFHILWFAVWIIFGVEGYPFGLLTMIVSLEAIFLSTFVMISQNRAD
                     FKRQAAADLQWKMVQEEDDQNKQLIALSNQILELTKAIHAHTKATQSVKQ"
     gene            complement(6129..7535)
                     /locus_tag="VFC01_22795"
     CDS             complement(6129..7535)
                     /locus_tag="VFC01_22795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HZN82479.1"
                     /translation="MAATDPTFYRSPGAAVAAAPEQLAYVVAFDPAGQAKDALAVVDC
                     DSASSSYGQVVGWSELPTAGNELHHFGWNACSSALCHEGHDDHSMERRYLLIPGIRSS
                     NTYVLDTKPDPRNPVLAHTIGADELAAKAGYSRPHTVHCGPGGIFMSALGGPNGDDGP
                     GGVALLDHDTFQVIGPWEKDRGPQQFAYDVWWHLRHDTAITSEWGSPSMIENGLNPED
                     LLGRRFGHHLNFWSLSEGRLTQRVDLGDAHQMVLELRPAHDPSKTWGFAGVVISAEDL
                     SASVWLWHQDGETWAVDKVITIPAEPAEADDLPPALKPFGAVPPLISDIDLSVDDRWL
                     YVSAWGTGELKQFDVSDPFHPRETASARLGGVVRREPHPAEPELRLAGGPQMIEISRD
                     GRRVYITNSLYAAWDEVFYPDGVGAWMAKLDADIDAGGLVPDAGFFPHGDEFRGLRVH
                     QTRLQGGDASSDSYCFTD"
     gene            7603..8022
                     /locus_tag="VFC01_22800"
     CDS             7603..8022
                     /locus_tag="VFC01_22800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82480.1"
                     /translation="MVSCGKGRWVVGLADFGGADVPGAGAALVCRGFGGGRVVLGSTG
                     AGGLADGAITGVCGGACVAAGALVGAVVGAGGGGVMVTGIVGTTAPVGPTGSRDTTRT
                     ADTAMATATSAMALSAITAVAVPYQGSREGSSKASQS"
     gene            complement(8047..8838)
                     /locus_tag="VFC01_22805"
     CDS             complement(8047..8838)
                     /locus_tag="VFC01_22805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82481.1"
                     /translation="MKARLLAVTAAVSLTAVSTACASRPSTPPAASAPNTSSSVSAPP
                     SPGPMKITDPYSPTIEPSAFTTKIDNPYFPLSPGTRTIYEAATKDGLQRTTTEVTRDT
                     KKVMGVDTVVVHDTVSVDNEPLEDTLDYYAQDRDGNVWYFGEATKEFKDGTVDTAGSF
                     EGGVDGALPGVIMPGHPQIGDQYRQEYAKGVAEDTGEVLSLSGSETTPLTGPAKDLLV
                     TKDSDLLDPTGPGENKYYARGFGLILTVTTTGPPERDQATVVEKF"
     gene            complement(8886..9263)
                     /locus_tag="VFC01_22810"
     CDS             complement(8886..9263)
                     /locus_tag="VFC01_22810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HZN82482.1"
                     /translation="MTTWDDVARIVIELPETTEASPRTWRVRKKLIAWERPLRKADYA
                     ALGADAPDGDILGARVAEEGVKFALIADDPGVYFTTPHFDGYPAVLVRLAEIGLPELE
                     ELITEAWLAQAPKTLSRSFLNGS"
     gene            complement(9273..9614)
                     /locus_tag="VFC01_22815"
     CDS             complement(9273..9614)
                     /locus_tag="VFC01_22815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82483.1"
                     /translation="MSSIGFVVPVSPDKEQADREWMSALDGDHRDEYRSAWKDAGFKR
                     HTVWQQQTADGTVDIVYLEADDIPAAMQAVTSSDSPFHQWFRERVLDVHDLDLAKENP
                     PLPTLLHDASF"
     gene            9708..>10591
                     /locus_tag="VFC01_22820"
     CDS             9708..>10591
                     /locus_tag="VFC01_22820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZN82484.1"
                     /translation="MGIKVALEHRTSYTFDRLVEVYPHVVRLRPAPHSRTPIEAYSLQ
                     VEPADHFINWQQDAFGNFLARLVFPNRARGVTITVGLIADMKVINPFDFFIEDWAEHI
                     PFEYPNALAEDLKPYLRPVDEGGEGSGPGDLTQAWVRNFSVAPGTRTIDFLVALNQAV
                     NADVGYNVRMEPGVQTPDFTLRTGIGSCRDSAWLLVSVLRQMGYAARFVSGYLVQLTS
                     DVEALDGPSGPAADFTDLHAWAEVYIPGAGWIGLDPTSGLFAGEGHIPLSATPHPESA
                     APITGATEPCETTLDFSNV"
BASE COUNT         1767 a         3407 c         3488 g         1929 t
ORIGIN      
        1 gtcctttcga aggcaatgag ctgctgctgt tgacgacgac gggcgcgaag tccggtgagc
       61 cacgagtctc gccgctgtcc tgcaagcgca tcgacggcaa gctgctgatc atcgccggaa
      121 acggcggtgc ggagaccaac ccggcgtggg tgtacaacct gcgggccaac ccccgcgcgc
      181 acgtcgaact gggcaccgaa tcgttcgatg tgacggcgcg ggaactgccg cccgccgagc
      241 gtgacgacat cgttcccaaa ctcaccgccg aggtatcggc gttcgcggaa ttccaggcga
      301 agacccgccg agtcattccg atcttcgaat tgcagccggc ctagcgatgc ttcgcatcgg
      361 aatcatgctg cccagccgcg agaccgcgat gacgggcgag cacaacgcgc ggggactcgt
      421 cgaattcgcg aagacggcag aagaggccgg gttcgactcg gtgtgggtgg gcgactcact
      481 gctcgcgcgc actcgcgcgg agccgctgtc ggtcctgtcc gccgtcgcgg cggtgacgga
      541 tcgggtggtg ctggggatcg cggcgttgat cgcgccgctg cgtcatccgc tgcttgccgc
      601 ggcgcaagcg gctaccgtcg accagctctc gggtggccgg ctgattctcg ggctgggttc
      661 gggcgccccg ctgccggaga gccaacgcga attcgacgcg gtgggaatgc cgttcgccgg
      721 tcgcggcgcc cgcctcgaca agaccgtcac cctgtggcgc gagacatggg ccgggcaagg
      781 cgaattcgcc gacgcgctgc ctaccgcggg accggatggt ccgccggtgt ggctggcggg
      841 tggcgatacg acgaaagtca tcgagcgggt agcggcgacc tacgacggct ggctgccgta
      901 cctgccggac gtgaatgcgt atgcgcgggc gtgggatcgc atcagggcgc aggccgttcg
      961 cgaggtcacc ccggcgttgt acgcgaccgt caacctcagc ggcgaacgcg ccgatgatga
     1021 actcgacgag tatctgcgtg cctactacgg ccagccgctg gaggtcatgc gctatgtgca
     1081 ggcgatccgc ggcggctcgg tcaatgagtg tgtggactgg ctggcacgtt acgtcgacgc
     1141 gggcgcacaa cacctgatcc tgcggatagg gtcgctaacc gcgcgacccg aatccgtggc
     1201 tgaacgatta ctgccggcac tgaaggagct tgcatcgcca tgaccgaaat acctgacgcc
     1261 gagaccatga aggcgttcaa caagaacatc gtcgacgagt ttcgcgccaa cggcggcaag
     1321 gtcggcggac cgttcgaagg cgcgaccctc ttgttgctga cttcgaccgg tgccaagtcg
     1381 gggcagccgc ggttggcgcc gctggcttac ctgacgatcg acggcaagac gatcatcatc
     1441 ggctcgaagg cgggcgccga cacgaatccc gattgggtgc acaatcttcg ggccaatcca
     1501 cgcgcgcata tcgaggtggg cgccgacggg tacgacgtga tcgcccgcga gctgccgccc
     1561 gaagagcgcg acgcgacgta tccgaaggtg gtcgcgttgg cgccgggttt cggtgagtat
     1621 caatcgaaga cgagccgcgt cattccgctg ttcgagctgc agcgcaccta acgcgcatgc
     1681 tagcggcgaa tctgggagca ccatcgcttc cacacatgga gtaagccgtg tcagcgtgta
     1741 tcgggtactt gctgaaagca ttgattgatg acgacgacaa cgaatgatca gcgactttcg
     1801 agcgtcagtt tgttggttcg aattcccata acacgggaga ccccttgggt cccttcggta
     1861 tcggaaagta agtgtggtgg gtagttgggt cgagggccag tgagtgtgcc ccgtcggcga
     1921 ggtgggctga acctcgctcg gtcaggtgtc cgtgttgatg ttcgaaaatg ctcacccagc
     1981 cgctctcggt ggccacatag acacgcccag cgccagcatc gtaggccaac acatcgggcg
     2041 tttcgccgac tccgtggtgg tcaactacag ttcggcttga cagatcgacg gtgaccatcg
     2101 tcgcgttggc ttcgcagccg acgaacatca cctgatcggt gacgtcgaga gcctggccgt
     2161 gcgggtgatc gcaaccgggc gtgggaatcc gttcagtcac ggtgaaactg acagggtcaa
     2221 tgacggcgag ttcgttgcga ccctgcaccg caataaccat ctggtcgagg aagttgtcgt
     2281 atacgacgtt ccctacctca ccgcccaacg gaacggtcgc gcggactgct ccggtgtcgg
     2341 cgtcgataac tgtctcactg cccgcactct cgtttgtcgt ccacacagtc cgccggactg
     2401 ggtcgtacgc cagtccgtcg ggataggtgt ctgtcggagc gcggaacaca acctggcctg
     2461 aatctgcgtc gatcgcagcg agctggttgt tgccggtcgc cgtagcgtat acgcgatgct
     2521 tgccggggac gacgatcaca ccatgcacgt cgggcaggtc aggcactgtg cgcactaccc
     2581 cgtgggcgtg cacgtcaact tcgatcagtt cacctgcgcc catatgggca atgaacaacc
     2641 gtccccgctc gacatctagc gccgtgtagt caaaccgaac tggaccaccg gtcagtgcgg
     2701 tctcagatat ctgccgtagc ggcaacccac cacccccctg cgatgatgtc gagcatccag
     2761 ccagtgcggc gtagacgatc gcaccggcga ccacccacgc gcgccttcgc acggcaccca
     2821 cccttcgcaa ggacaaaccg cgcttcaaag ctacgccgtg aacgggatac ggaacgttgg
     2881 gggacgccct ttgccacagc cacatcacgc ctcgtacggc tggcgaaggc tctctccggg
     2941 ggagcacccc ggttgtgcgg tccagtgagg ctgttctgcg acatcgcccg aatagatcac
     3001 cgatggaaac tgaatcccat gctgtcctca caacaagccc taagtcctca gttggtcgtt
     3061 gtgtgcgtcg ccatggtgat cgctgccgct ctggtctact ggttgactgc gctcggttcg
     3121 ccgtggactg tcccatgggc ggctgtgcgt gcagcgatgc agctggccgc tgtcgcgggc
     3181 gtgctggcgg ccgctcttgc gcggttgtgg tcgtcgattc ttgtcttggc ggtgatgttt
     3241 gttgtcgccg gcttgaccgc cgctcggcgc agccaggcca gccgtgggtc tccattgttg
     3301 tcggtgtcgc tcgcgcttgg catggtcgcc gtcttgcctc tgttgttgtt ttccggcgtc
     3361 gtaccgctga ccggcgtggc agtcgtgccg atagtcgcca ttgtgctcgg caacaccatg
     3421 actgccgtag cagtcgccgc ccgtttggcg ctggatgtcc tacagctgcg agccggcgag
     3481 gttgaagccg cgcttagcct cggtatgagc gagcgggact cgcggatgga gattatcggg
     3541 caaacagctg ccaacgcgtt gctacccaat ctcgattcga ccagaaccgt tggcctcgtc
     3601 acgcttccgg gtgccttcgt gggcgtgctg ctaagtaccg gatcggccgc ccaagccgct
     3661 gccgtgcaaa tcctggttct tatcgcgctg ttgctctgtc aaacctgcgg ggtcgccgcg
     3721 accatcgaac tgatcgcacg cggcatgatc gcgcggcaac ggtgaattcg cctgtggaac
     3781 atctaagccg gacccgtccg gccacgtaag ttgaagattg cactcaaggt cgtgaaaggc
     3841 accagatcac gaccctgagt gcaatcggga ggcaagcttt tacagccgct cgatgatggt
     3901 ggcgttggcc atgccgccgg cctcacacat cgtctgcaac ccgtaccggc cgccgcgctg
     3961 ttcgagcgca ttgaccatcg tcgtcatgat gcgcgcaccg ctggcaccca gcgggtggcc
     4021 gatcgcgatc gcaccgccgt tgacgttcgt cttagccaag tcagcgccga catcgtgggc
     4081 ccacgcgagc acaacgggcg cgaacgcttc gttcacctcg aacaggtcga tgtctgaaag
     4141 cgtcaatccc gcacgctgca agaccttttc ggtcgcgggg atgacaccgg tcagcatgta
     4201 aagcgggtcc gatccgacca cggtcgccgt gtggatccgg gccagcggac ggagcccgag
     4261 ccgcgccgcc acctcactgg tggtgatcaa caccgcggcg ctgccgtcgg acaacggcga
     4321 cgagttgccc ggagtgatct cccacttgat ctgcgggaaa cgggcctcgt aggccggggt
     4381 gtagaacgcc ggcttcagcc cggcaagggt gtcgacagtc gtcccgggcc ggatgatctc
     4441 gtcggtcgtc agcccggcga tcggaatcag ctcattgtcg aaccggcctt ctttcgttgc
     4501 cgcggcggcc ttttcgtggc tcccagcgga gaactcatcg agctgctgtc gcgagaagcc
     4561 ccatttggcg gcgatcagct ctgcgctgat gccctgcggc acaaggcctt cgggataccg
     4621 ctgcgccatc gcgccgaacg ggtcgctgcc cggcagcaca cttgagccca tcgggacccg
     4681 tcccatcgat tcgacgcctg cggcgatcac gatgtcgtag gcgcccgaga tgacgccctg
     4741 ggcggcgaag ctgatcgcct gctggctgct gccgcactgc cggtcgacgg tcgtgccggg
     4801 cacactctcg gggaatccag cgccaagcac tgcgttgcgt gcgatgttga ctgcctggtc
     4861 gcccacctgg gtgaccgcgc ctgcgatgac gtcgtcgatc tgcgccggat cgattccggt
     4921 ccggttgacc agttccttga ggctgtgcgc cagaaggtcg acgggcagga cgtcgtgcag
     4981 cgcgccgccg gccttgccct tgccgatcgg ggtgcgtacc gccccgacga ttaccgcgtc
     5041 ccggcctgcg tatccggcca ttgttgcctc ctgtactcga ctctgagtag agctctctat
     5101 atccagctat aacacctagg tatagtgata acaacccaga aaaggagtga cgtgcatgac
     5161 agtgctgcag ggacagctcg ccgaccgtga cgcctggtcg gcggtgggca agtgtcccat
     5221 cgagaagacg atggcattgc tgggcaccaa gtcggcaatg ctgatcatgc gggaggccta
     5281 ctacggcacc acgcgattcg aggacttctg gcggcggatc ggcgtcacca aggccgcggc
     5341 gtcggcccgg ctgaacgaac tggtggaggc cggcttgctg cagcgcaggc cctataagga
     5401 gcccggccag cgcagccgcg acgagtacgt gctctccgat gccgggctcg atttcatgcc
     5461 ggtcatatgg gcgatgttcg agtggggccg tcgccacctg ccgtacagcc cgcagctgcg
     5521 gctcgcccac agggactgcg gcgccgaggc taccgtcgaa atccgctgcg aaaagggcca
     5581 tcacgtgccc gccgacgaac tcgtggttca gctcgcccag agctgacccg cagcggattc
     5641 acagctgaag gtaacgggac cggggtttgc ccacagctac cgtgagccat gtcaaacacc
     5701 accacagctc acccggtgca ccacgggttc gtgcccccga agctgcccaa gccgatcaac
     5761 ccggcgctcg ccaagcatta cgaggcccgc gccgcgagca tccagaatcg gattgccgat
     5821 gcgataaccc gattcgccgg gtcgatggcg ttcgtgtact tccacattct gtggttcgcg
     5881 gtgtggatca tcttcggcgt cgagggatat cccttcggcc tgttgacgat gatcgtgtcg
     5941 ctcgaggcga tcttcctgtc gaccttcgtg atgatcagcc agaaccgcgc cgacttcaaa
     6001 cgccaggcgg ccgcggatct gcaatggaag atggttcagg aagaggacga ccagaacaag
     6061 caactcatcg cgttgtcgaa ccagatcctc gagctgacga aggcgatcca cgcccacacc
     6121 aaggcgactc agtcggtgaa gcagtaggaa tcactcgacg cgtcaccgcc ctgcagccgg
     6181 gtctggtgca cccgcaggcc gcggaactcg tcgccgtgcg ggaagaaccc ggcgtcggga
     6241 accagcccgc ccgcgtcgat gtcggcgtcc agcttggcca tccacgcacc aacgccgtcg
     6301 gggtagaaca cctcgtccca tgccgcgtac agcgagttgg tgatgtagac gcgcctgccg
     6361 tcgcggctga tctcgatcat ctgcggcccg ccggcgagcc gcagctcggg ttcggccggg
     6421 tgcggttcgc gccggactac gccgccgagg cgagccgacg ccgtctcgcg cgggtggaac
     6481 gggtcgctga catcgaactg cttgagttcg cctgtgcccc aggctgatac gtacagccaa
     6541 cgatcgtcga ccgacaggtc gatgtcggat atcagtggag gcaccgcgcc gaacggcttc
     6601 agcgccggcg gcaggtcatc ggcctctgcg ggctcggcgg gaatcgtgat gaccttgtcg
     6661 acggcccagg tctctccgtc ttgatgccac aaccacaccg aggccgacag gtcctccgcg
     6721 ctgatcacca cgccggcgaa cccccacgtc tttgacgggt cgtgcgcggg ccgcagttcc
     6781 agcaccatct gatgggcgtc gccgaggtcg acgcgctgcg tcaacctgcc ttctgacagg
     6841 ctccaaaagt tcaagtggtg cccgaacctt cggcccagca ggtcctcggg attcagcccg
     6901 ttctcgatca tcgacggcga gccccattcc gaggtgatcg ccgtgtcgtg gcgcagatgc
     6961 caccacacgt cgtaggcgaa ctgctgcggt ccgcggtcct tctcccacgg cccaatcacc
     7021 tggaacgtgt cgtggtcgag cagcgcgaca ccgccgggcc cgtcgtctcc gttcggtccg
     7081 ccaagcgcgg acatgaagat gccgcccgga ccgcagtgca cggtgtgcgg ccgtgagtag
     7141 ccggccttgg cggcgagttc gtcggcgcca attgtgtggg ccaacaccgg gtttcgtggg
     7201 tcgggtttgg tgtcgaggac atacgtgttc gacgaccgga taccggggat caggaggtag
     7261 cgccgctcca tggaatggtc gtcgtggccc tcgtggcaca gcgcgctcga gcaggcgttc
     7321 cagccgaagt gatgcagttc gttgccggcc gtcggcagct cggaccagcc gaccacctga
     7381 ccgtacgatg acgatgcaga gtcgcagtcc accacggcga gcgcgtcttt cgcctggccg
     7441 gccgggtcga atgcgactac gtaagcaagc tgctcgggtg cggcggcgac ggccgcaccc
     7501 gggctgcggt aaaacgtggg atctgttgca gccataccag cagatcatcc tccgcgaggc
     7561 cgagcaaatc agtgattcgt cccaggtggg ccaaggaagt cggtggtctc ctgtgggaag
     7621 ggccgatggg tagtcgggct ggcggatttc ggcggcgcgg atgtgcccgg agccggggca
     7681 gcgctggttt gccgtggctt cggcgggggc cgtgtggtgc tgggttcgac cggtgccggc
     7741 gggcttgctg acggggcgat caccggcgtt tgcggcgggg cctgcgtggc ggccggcgcg
     7801 ctcgttggcg ccgtggtcgg ggccgggggt ggcggcgtga tggtgaccgg gatcgtcggc
     7861 acgacggcac cggtggggcc caccggctcg cgggacacca cgagaaccgc cgatacggcg
     7921 atggcgaccg cgacgagcgc gatggcactg agcgcgatca ccgcggttgc cgtgccgtac
     7981 cagggttcgc gcgagggctc atcgaaggcg tcgcaaagct gacgatcacc gtcgagcaca
     8041 gtcacgtcag aacttttcga cgacggtggc ttggtcgcgc tcgggcggac ctgtcgttgt
     8101 cacggtgagg atgagcccga aaccgcgggc gtagtacttg ttctcgcctg gcccggtggg
     8161 atccagcagg tcggagtcct tggtgacgag caggtccttg gcgggcccgg tcagcggggt
     8221 ggtttcactg ccgctgagac tcagcacctc gccggtgtcc tccgcgacgc ccttggcgta
     8281 ctcctggcgg tactgatcgc cgatctgcgg gtgtcccggc atgatgacgc cgggcagggc
     8341 accgtcgacg ccgccctcaa acgatccggc ggtgtccacg gtgccgtctt tgaactcctt
     8401 cgtggcctcg ccgaaatacc agacattgcc gtcgcggtcc tgggcgtagt agtccagcgt
     8461 gtcctctaac ggctcgttgt cgacggagac cgtgtcgtgg acgacgacgg tgtcgacacc
     8521 catgaccttc ttggtgtcgc gagtgacctc ggtggtcgtg cgttgcagac cgtccttcgt
     8581 ggctgcctca tagatcgtgc gggtgcccgg gctgagcggg aagtacggat tgtcgatctt
     8641 ggtcgtgaac gcagacggct cgatcgtcgg cgagtagggg tcggtgatct tcatcggccc
     8701 gggactcggc ggggccgaaa cagaactcga agtgttgggc gcggaagcgg ccggcggcgt
     8761 ggaggggcga ctcgcgcacg ccgtgcttac cgcggtcaac gacaccgctg cggtcaccgc
     8821 caacagacgg gccttcatgt ggcacaccgt aactccgctg agggccaatt gtcgcccagt
     8881 gcttttcagc tcccgttcag gaagcttctg ctaagcgtct tcggggcctg cgccaaccag
     8941 gcttccgtga tgagctcctc gagctcaggc agcccgatct cggccaggcg caccagcacc
     9001 gccgggtagc cgtcgaaatg cggagtggtg aagtacaccc ccggatcgtc ggcgataagc
     9061 gcaaacttca cgccctcctc ggccaccctg gcaccaagaa tgtccccgtc aggggcatcg
     9121 gcgcccagcg cggcgtagtc cgccttgcgc agcggtcgtt cccaggcgat cagcttcttg
     9181 cgcacccgcc aggtgcgtgg tgacgcctcc gtggtctcgg gaagctcaat gacaattcgt
     9241 gcgacgtcgt cccacgtggt cacgccatca gactagaagc tggcgtcgtg caacagcgtc
     9301 ggaagcggcg ggttctcctt cgccaggtcg aggtcgtgca catcgagcac ccgttcgcgg
     9361 aaccattggt ggaacggcga gtccgacgac gtgacggcct gcatggccgc tggtatgtcg
     9421 tcggcctcca ggtacacgat gtccaccgtt ccatcggcag tctgttgctg ccagacggtg
     9481 tgccgcttga agccggcgtc cttccatgcc gagcggtatt cgtcccggtg gtcaccgtcg
     9541 agcgcgctca tccattcgcg gtccgcctgc tccttgtctg gcgaaacggg cacgacaaag
     9601 ccaatcgaac tcatcaccca ccccccgtga attagctgtg ggatgagcgt acgcccgtgt
     9661 ggtgcgcgtg cgatgcgagc gggcatacct tgtgatggga tctgcagatg ggcatcaagg
     9721 tggcgctgga gcatcgcacc agctacacgt tcgatcgact ggtggaggtg tacccccacg
     9781 tcgttcggct gcgtcctgcg ccgcactcgc gtaccccgat cgaggcctac tcgctgcagg
     9841 tcgagcccgc cgatcacttc atcaactggc agcaggacgc gttcggaaac ttcctggccc
     9901 gcctagtttt cccgaaccgt gcccgcggcg tgaccatcac cgtcgggctg atcgccgaca
     9961 tgaaggtcat caacccgttc gactttttca tcgaggactg ggccgaacat atcccgttcg
    10021 aataccccaa cgcgctcgcc gaagacctca agccctatct gcgcccggtc gacgagggcg
    10081 gcgagggctc ggggcccggc gacctcacgc aggcgtgggt gaggaacttc tccgtcgcgc
    10141 ccggcacccg caccatcgac ttccttgtcg cgctcaacca ggcggtcaac gccgacgtcg
    10201 gctacaacgt gcggatggaa ccaggtgtgc agacgccgga tttcacgctg cgcacgggca
    10261 tcggttcgtg tcgcgactcg gcgtggctgc tggtgtcggt cctgcgccag atgggctatg
    10321 cggcccgctt tgtgtccggc tacctggtgc agttgacctc cgacgtcgag gcgctcgacg
    10381 ggccgagcgg gcctgccgcc gacttcaccg atcttcacgc ctgggccgag gtgtacatcc
    10441 ctggcgccgg ctggatcggg cttgacccga cgtcgggcct gttcgccggc gagggtcata
    10501 tcccgctgtc cgcgacgccg catcccgagt cggcggcgcc gatcaccggc gcgaccgagc
    10561 cgtgcgagac cacgctggac ttctccaacg t
//
LOCUS       DASRJA010000523         9463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4013190,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000523 DASRJA010000000
VERSION     DASRJA010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4013190"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFC01_22825"
     CDS             <1..427
                     /locus_tag="VFC01_22825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HZN82485.1"
                     /translation="RRGLEQQRIFADEGVDLTRVVIGHSGDTTDLDYLEELIGNGSYI
                     GMDRFGVDVFLPFEDRVNTVARMCERGHANKMVLSHDAACFMDWVPEQLLPALPNWHY
                     LHIHNDVIPALKERGVTDEQLTTMLVDNPRDIFENQGGY"
     gene            427..1896
                     /locus_tag="VFC01_22830"
     CDS             427..1896
                     /locus_tag="VFC01_22830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN82486.1"
                     /translation="MTAPVPPIGTQFSRLAELAPDEPAVTCEGRTLTRGELDVSTNRL
                     ARAYAELGVRQGDYVTIVLPNSIEWVQAALAAWKLGAVPQPLSARLPDAELEGLLELK
                     PRALLVGRDDPRGVIPSVPGDFSPTPGVSQAPLPEAVSPTWKALGSGGSTGRPKLIEA
                     GGDSRFPGALVGLGMGGQDGDTQLIAVPLSHNTGMTTATIGLLLRNHLVLMPRFDAAE
                     FLRLVAEHRVDFIVAVPTIMQRLLPVYRANPDAYDLSSVRRLWHVGAACPPAVKQAWI
                     ELLGPEVVWELYGGTELQAITVLSGTQWLEHPGSVGVVVAGEMKVLDDDGNECPPGVV
                     GEIYMRPSPGEAPTYRYVGATAKSRDGWDSLGDLGYFDSDGFLYLNDRRVDMFTVGGR
                     NVYPAEIESALSAHPNVLSCLVVGVPDDDLGQVPYAIVQADGLDEAAVIAFLADRIAG
                     YKVPRTVEFSDTPLRDDAGKARRSAVRDEVIARRARAHS"
     gene            complement(1865..2977)
                     /locus_tag="VFC01_22835"
     CDS             complement(1865..2977)
                     /locus_tag="VFC01_22835"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZN82487.1"
                     /translation="MRACAAIGVVITHVAFQTGHTGGITGRLFGRFDLAVAVFFALSG
                     FLLWRGHAAAARGLRQVPPTGHYLRSRIVRIMPGYLVAVVVILTLLPDAKADLTVWLA
                     NLSLTQIYVPLTLTAGLTQMWSLSVEVAFYLALPILALFARRLPVRARIPVIAAVAVA
                     SLGWALIPFDAPFGVNPHNWPPAFFAWFAAGMLLAELTVTEIGWPHRLARRRVLMAMV
                     AGVAFLVAASPLAGPEGLTPGTVSQFIVKTAMGALVAAALIAPLVLDRPDTPHRMLGH
                     TTMVTLGRWSYGLFIWHLAALAMVFPVIGEFAFNGHMPVVLVLTLLFGFAIAAVSYAL
                     VESPCRVALRRWEFREERPVPPLDSAVRNVPVPVSR"
     gene            3261..4457
                     /locus_tag="VFC01_22840"
     CDS             3261..4457
                     /locus_tag="VFC01_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3068 domain-containing protein"
                     /protein_id="HZN82488.1"
                     /translation="MNRAVALRIAACGIMGLGAALLIAALLLSTYTKGKIAKIPLDVD
                     ATLVADGTGTAFDPESLNADKFVINRNVPISQQEQVSVESPSNAEVITLQVGTSVRRT
                     DKQQDAGLLLALVDTVTVNRKTATAVSSESNPGGAVQKPRAIDETAPSTNIALPHDGL
                     AYRFPFDTQKQTYPVFDPIAQKAYDANYDGEEDVNGLTTFKFSQNVGYDADGKLVEPI
                     KYSSLYDDDADAEVTARASLWGLPGNPDESITMTRYYAAQRTFWVDPVSGTIVKKKEH
                     GYHYYAREPLKPEVTFVDFTVTSNEQTVESQVTSARDESDMLALWGRILPITFTAMGL
                     VALVGGALLGSFSLRAESALIDPGLDEADHRFFDTQGIKVPGAEAKTEKLPTPRPTDL
                     PPDRPV"
     gene            4454..6136
                     /locus_tag="VFC01_22845"
     CDS             4454..6136
                     /locus_tag="VFC01_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82489.1"
                     /translation="MSARFARWTAPAYALLLALLVTAPLLAPGYLLLRDAVSTPRSYL
                     SDSALGLSEAAPRALPQDFFVALASAVVDGGVVVKALLVFGLFVAGWGAAQLTARVLP
                     EAGLAGQVVAVTIAIWNPYVAERLLQGHWSLLVGYGCLPWVATAMLRLRTSEASRWAS
                     ACALVFWIAVAGLTPTGLMLAATVAVACVFAPGSGRPRWWCAAVGLGAAVLAALPWLT
                     AAAVADSLSSSQAEGVRAFATRAEPGLGTLGSLASLGGIWNADAVPATRTTLFAVIAA
                     VVLLGVVALGMPTVVRRPLAVPLLILAAVAVIVPAAMATGPGMAVLETTIRAVPGLGI
                     LRDTQKWVALAVPGYVLASAAAVVTVRPRLPSAATALVCCAALIATLPDLAWGVGNKV
                     VPVQYPTGWANAAAMINADPRPVAVLPVDSMRRYAWAGDAPVLDPLPRWVRADVLQAG
                     DLTIGGRTVPGEGVRARDVQQLLVTGADHDKLAAAGVGWVVVENVWHTGWSAPAVPLP
                     VAYHDEDLTLYRVGGDHPHPSGRGIMLAAHWVWLGLLVLSGLLAMGLARRRR"
     gene            complement(6199..6381)
                     /locus_tag="VFC01_22850"
     CDS             complement(6199..6381)
                     /locus_tag="VFC01_22850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82490.1"
                     /translation="MRARIAHPTLWVVDPRGRDTNLATVTDDRALFGAYPLADGEDTD
                     AETFGSLSESEQWLYW"
     gene            6368..6619
                     /locus_tag="VFC01_22855"
                     /pseudo
     CDS             6368..6619
                     /locus_tag="VFC01_22855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005113914.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(7070..7966)
                     /locus_tag="VFC01_22860"
     CDS             complement(7070..7966)
                     /locus_tag="VFC01_22860"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HZN82491.1"
                     /translation="MEPSEVIGTISELWRFPVKSMQGERLDQAIVGEAGILGDRAYAV
                     IDRETGKVASAKHPKLWPELLSCRATFSEPPRLGEKLPSVRIDLTDGTSVMSDADDVD
                     AVLSRFFGREVKLARSAPSDFTIDQYHPDIENLDPEGHRDEVTDTKLGAALFSQLGMA
                     SLVPEGSFMDLFPVSVITTSTLDRLSELLPESLLDIRRFRMNLFVDTAGAGFVENEWL
                     GQMIGVGSEVQLAVMLPDPRCVMTTAAQPGLPHDAAILRSLAKHNRLDVAGGGLYPCA
                     GVYAAAVAGGTVCTADPLRISA"
     gene            8426..8905
                     /locus_tag="VFC01_22865"
     CDS             8426..8905
                     /locus_tag="VFC01_22865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82492.1"
                     /translation="MKTQIIAALTIATAAALPLAAVANACGGGGGDVPTAYDFVSPSG
                     NIACTIYADGSGANCEVQEHTWVIPASTEGPYDRPCNFNFGGLAIYVSQGKPGKLGCY
                     EGSGKFKYPDPDQQTLDYGQTYSRGAITCDSEPSGVTCTDTVTRHFFRVSRESYEVG"
     gene            complement(8924..>9463)
                     /locus_tag="VFC01_22870"
     CDS             complement(8924..>9463)
                     /locus_tag="VFC01_22870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN82493.1"
                     /translation="SRLVPHKQIEDALEAIAALRPRMPDLHLDIVGGGWWEERLVEHG
                     ALLGISDAVTFHGHVDDETKHRVLQHSWVQVLPSRKEGWALAVTEAGQHSVPTIGYRS
                     SGGLTDSIVDGVTGLLVDNHHELVDQLERLLTDQVLREQLGAKAQTRSGEFSWRQSAD
                     AMRSVLEAMHAGHMVSGVV"
BASE COUNT         1630 a         3104 c         3130 g         1599 t
ORIGIN      
        1 ccggcgggga ctcgagcagc agcgtatctt cgccgacgag ggcgtcgatc tgaccagggt
       61 cgtgatcggc cactccggcg acaccaccga ccttgactac ctcgaggagc tcatcggcaa
      121 cggctcctac atcgggatgg accggttcgg tgtcgatgtc ttcctgccat tcgaggaccg
      181 ggtgaacacc gttgcacgga tgtgcgagcg cgggcatgcc aacaagatgg tgctgtcgca
      241 cgatgctgcg tgcttcatgg actgggtacc agaacaactg ttgccggctc tgccgaactg
      301 gcattacctg cacatccaca acgacgtcat ccctgcgctg aaggaacgcg gcgtgaccga
      361 cgagcagctg acgacgatgc tcgtcgacaa cccgcgagac atcttcgaaa accagggcgg
      421 ttactgatga ccgcgcccgt cccgccgatc ggcacccaat tctcccggct tgccgaactg
      481 gcgcccgacg agcctgcagt cacctgcgag ggacgcaccc taacccgcgg cgagctcgac
      541 gtgtcgacca accggctggc acgcgcctac gccgaacttg gtgtgcgaca gggtgattac
      601 gtgacgatag tgttgcccaa ttcgatcgag tgggtccagg ccgcattggc cgcatggaag
      661 ctcggtgcag taccccagcc gctctcggcc cggctgcccg acgccgaact cgagggcctg
      721 ctcgagctca agccgcgggc gctgctggtc ggccgtgacg acccgcgcgg cgtaattccg
      781 agcgtgccag gcgatttcag tccgacgcca ggagtgtcgc aggcgccgct gcccgaagcg
      841 gtgtcgccaa cgtggaaggc gctgggttcc ggcggcagca ccggtcggcc caagctcatc
      901 gaggcgggcg gcgacagccg cttccccgga gcactcgtcg ggctgggaat gggcggccaa
      961 gacggcgaca cacagctgat cgcggttccg ctgagccaca acaccggcat gacgaccgcg
     1021 acgatcgggc tgttgttgcg caaccatttg gtgttgatgc cgcgcttcga cgccgcggag
     1081 tttctacggc tggttgccga gcaccgggtc gacttcatcg tggcggtgcc caccatcatg
     1141 cagcggctgc tcccggtcta tcgggccaac cccgacgcct acgacttgtc gtcggtccgg
     1201 cggctgtggc acgtcggtgc ggcgtgcccg cccgccgtca agcaggcgtg gatcgagctg
     1261 ctcgggcccg aggtggtgtg ggagctctac ggcggcaccg aacttcaggc catcacggtc
     1321 ctcagcggca cgcaatggct cgagcacccc ggctcggtcg gcgtcgtcgt cgcgggcgag
     1381 atgaaggtgc tcgacgacga cggaaacgag tgcccgccgg gcgtggtcgg cgagatctac
     1441 atgcggccat cgccaggtga ggcgccgacc taccgctacg tcggcgccac cgcgaagagt
     1501 cgcgacgggt gggactcact gggcgatctc ggctacttcg actccgacgg cttcctctat
     1561 ctcaacgacc gccgcgtgga catgttcaca gtcggcggtc gcaacgtcta ccccgccgag
     1621 atcgaatcgg cgctgtcggc acacccgaat gtgttgtcct gcttggttgt tggtgtgccg
     1681 gacgacgacc tgggccaggt gccctacgcg atcgtgcagg ccgacgggct cgatgaagcc
     1741 gcggtgatcg cgtttctggc cgaccgcatc gccggctaca aggtgccccg tacggtggaa
     1801 ttcagcgaca cgccgctgcg cgacgatgca ggcaaggcgc gccggtctgc ggtgcgcgac
     1861 gaggtcatcg cgagacgggc acgggcacat tcctgacggc ggagtccagc ggcggcaccg
     1921 gacgttcttc gcggaactcc catcgccgca gggcaactcg acacggcgac tcgaccagtg
     1981 cgtagctgac cgccgcgatc gcgaagccga acagcagggt cagcaccagc acaacgggca
     2041 tgtgaccgtt gaatgcgaac tcaccgatga cggggaacac catcgccagc gcggcgaggt
     2101 gccagatgaa caacccgtag gaccaccggc ccagcgtgac catcgtcgtg tggcccagca
     2161 tccggtgcgg ggtgtcgggg cggtccagca ccagcggggc gatcagtgcc gccgcgacca
     2221 acgcacccat cgcggtcttc acgatgaatt ggctgacggt gccgggtgtc aacccttcgg
     2281 ggcctgccag cggtgaggcc gctaccagga acgccacccc cgcgaccatc gccatcagca
     2341 cccgccgtct ggccagccgg tgcggccaac cgatctcggt gacggtcaac tccgccaaca
     2401 acatgcccgc ggcgaaccat gcgaagaagg caggcggcca gttgtgcggg ttgacgccga
     2461 agggcgcgtc gaacgggatc agagcccacc cgaggctggc caccgccacg gccgcgatca
     2521 ctgggatacg ggcacgcacc ggcaatcgtc gcgcgaacag cgcgaggatc ggcagcgcca
     2581 gatagaaggc gacctcaacg gacaggctcc acatctgcgt cagaccggcg gtcagcgtca
     2641 gcggaacata gatctgcgtc agcgacaggt tcgccagcca caccgtcagg tcggccttgg
     2701 cgtcgggcag tagcgtcaga atcacgacga ccgcaaccag gtagccaggc atgatgcgca
     2761 cgatgcggga ccgcaggtag tgccccgtcg gcgggacctg ccgcagcccg cgcgccgcgg
     2821 cggcgtggcc acgccacagt aggaagcccg acagggcgaa gaacaccgca acggcgaggt
     2881 cgaagcggcc gaacagccgc ccggtgatcc cgccggtgtg accggtctga aacgcgacgt
     2941 gggtgatgac gacgccaatg gctgcgcacg cgcgcatccc ctcgacagcg ggcaggaagt
     3001 ccccctggcc tgcggccggg tgggccccca gcttgcgagg ggagtgcccc cagcgcgtcc
     3061 cgccgatctg tgaggcttca gtcacgacga ccagtgtgcc gtgctgatgg atcgacgtga
     3121 aaacgggtag tcaagcggcc gccggcggat gccttcctag taagggacgg ctttagtttg
     3181 tgctgttagg gtcgaacggg tttgccgtca ccccccagct aggggcgatc gtcgatggcg
     3241 gaaggtgaag gaggcacggc ttgaaccgcg cagtcgccct gcgtatcgca gcctgcggaa
     3301 ttatgggttt gggcgctgcc ctgctgatcg ccgcgctgct gctgtccacc tacaccaagg
     3361 gcaagatcgc caagatcccg ctcgacgtgg acgccacact cgtcgccgac ggaaccggta
     3421 cggcgtttga ccctgagtcg ctgaacgccg acaagttcgt gatcaaccgg aatgtcccga
     3481 tctcgcagca ggaacaggtc agcgtggagt cgccgtcgaa cgccgaggtc atcaccctgc
     3541 aggtgggcac gtcagtgcgc cgcaccgaca agcagcagga cgccggcctg ttgctggcgc
     3601 tggtcgacac ggtcacggtc aaccgcaaga ccgcgaccgc agtctcgagc gaaagcaatc
     3661 caggcggcgc cgtgcagaag ccgcgcgcca tcgacgagac tgcgccgtca accaacatcg
     3721 ctctgccaca cgacgggctg gcctaccgct tcccgttcga cacccaaaag cagacgtatc
     3781 ccgttttcga cccgattgcg cagaaggcat acgacgccaa ctacgacggc gaggaagacg
     3841 tcaacgggct gaccaccttc aagttcagtc agaacgtcgg ctatgacgcc gacggcaagc
     3901 tggttgagcc gatcaagtac tcgtcgctct atgacgacga cgcggacgcc gaggtcaccg
     3961 cccgggcatc gctgtggggg ctacccggca acccggacga atcgatcacc atgacgcgct
     4021 actacgccgc gcagcgcact ttctgggtgg accccgtgtc gggcaccatc gtcaagaaga
     4081 aagagcatgg ctaccactac tacgcgcgcg agccgctcaa gcccgaggtg acgttcgtcg
     4141 acttcaccgt cacatccaac gagcagaccg tcgagtccca ggtgaccagt gcgcgcgacg
     4201 agagcgacat gctggcgcta tggggccgca tcctgccgat cacgttcacc gcgatgggcc
     4261 tggtggcgct cgtgggcggc gcgctgctgg ggtcgttcag cctgcgcgca gaatcagcgc
     4321 tgatcgatcc agggctggac gaggccgatc accgattctt cgacactcag gggatcaagg
     4381 tgccgggggc cgaagccaag accgagaaac tgcccacccc ccggccgacc gacctgcccc
     4441 cggacagacc ggtctgagcg ctcgcttcgc ccgctggacg gcgccggcat acgcgctgct
     4501 gctcgctctg ctggtgacgg cgccgctgct ggcgccgggg tatctgctgc ttcgcgatgc
     4561 ggtatcgacg ccgcggtcgt atctgtctga ttccgcgctc gggttatcag aggccgcgcc
     4621 ccgggcattg ccgcaggact tcttcgttgc gctggcctct gccgttgtcg acggcggcgt
     4681 ggtggtcaag gcgcttctgg tctttgggtt gttcgtggcg ggctggggcg cggcacaact
     4741 aacggctcgg gtgctgcccg aggcgggctt ggccggtcag gtcgtcgcag tcacgatcgc
     4801 catctggaat ccctatgtcg cggaacgact tctgcagggg cactggagtc tcctcgttgg
     4861 ctacggctgt ctgccgtggg tggccacggc gatgttgcgg ttgcgtacgt cggaagcgtc
     4921 gcggtgggct tcagcgtgcg cgctggtgtt ttggatcgcg gtggccgggc tgaccccgac
     4981 cgggctgatg ctggcggcga cggtcgcggt ggcttgcgtt ttcgcgcccg ggtccggccg
     5041 gccgaggtgg tggtgcgcgg ccgtcgggct cggcgcggcg gtgctggccg cgttgccatg
     5101 gcttaccgcc gcggcagtgg ctgattcatt gtcgtccagt caggccgaag gtgtgcgtgc
     5161 gttcgccacc cgcgccgaac ccggtctcgg cacgctgggc agcctcgcca gcctcggtgg
     5221 catctggaat gctgacgctg taccggccac gcgcacaacg cttttcgcgg tgattgccgc
     5281 tgtggtgctg ctcggtgtgg tcgctttggg gatgcccacc gtggtacgcc gccccctcgc
     5341 ggtgccgttg ttgatccttg cggcggtggc ggtgatagtg cccgccgcga tggccaccgg
     5401 acccggtatg gcagtcctgg agacgacgat ccgggcagtg ccgggcctcg gcattctgcg
     5461 agacacgcag aagtgggtgg cgctggccgt gccgggctat gtgctcgcga gcgcggccgc
     5521 cgtggtcacg gtgcggcccc gactgccgtc ggccgcaacg gctttggtct gttgtgcggc
     5581 gttgatcgcg acgttgcccg acctggcctg gggcgtcggc aacaaggtag ttcccgtgca
     5641 gtatccgacg ggatgggcca atgcggccgc gatgatcaac gccgacccac gaccggtcgc
     5701 cgtgctgccc gtcgacagca tgcgccgcta cgcatgggcc ggcgacgcac ccgtgctcga
     5761 cccgctgccc aggtgggtac gcgccgacgt gctgcaagcg ggcgacctga caatcggggg
     5821 acgcacggtc cccggcgagg gtgtgcgcgc ccgcgacgtg caacagctgc tggtgaccgg
     5881 cgccgaccac gacaagctgg cggccgctgg cgtgggctgg gtcgtcgtcg aaaacgtctg
     5941 gcacacaggg tggtccgcac ccgcggtgcc cctgccggtg gcgtatcacg acgaggatct
     6001 cacgctgtac cgggttggcg gcgatcaccc gcacccgtct ggccgcggca tcatgctcgc
     6061 cgcgcattgg gtgtggctgg gcctgctcgt actcagtggc ctgctggcga tggggctcgc
     6121 tagacgacgt cgctagcagt cggcgatggc gacatgtcga tggtcgcata ctcgatagtt
     6181 caggattaca cccatgaatc accagtagag ccactgctca ctttcgctca agctgccgaa
     6241 cgtctcggcg tcggtgtcct caccgtccgc caatgggtac gcaccgaaca gtgcccggtc
     6301 gtccgtgacg gtcgcaaggt tcgtatccct tccgcgtggg tcgacgaccc acaaggttgg
     6361 atgagcgatg cgtgccctca tcgttgtccg actgagccgg gtcacggacg ccaccacctc
     6421 gcccgaacgc cagcttgcgg cgtgccggga gctgtgcgac cagcgcggct acgaggtggt
     6481 cggcgtagcc gaggacctgg acgtatcagc tgggtctagg tcgccgttcg accggcccca
     6541 ggccggggac tggctgtcga atcgcctcgg cgagttcgac gtaatcgtgt tctttcgagc
     6601 cgaccggatc gtccgccggc gtgctcgctg ctcagctccc ggatgagctt cgcaacttcc
     6661 agacgggacc gacatgttgc agcgcagggc cgacatcaca gcgtggattg acaagcgact
     6721 accgcggcgc ggggaggagt tggtcgagcc ctacctcgct gcgatccgcg accggctgct
     6781 gtatccgcat gtcgagagtc cggacgaaat tcgggctgct ctgatgacgt cgctgggcta
     6841 cggcctggag ccgggaccgg ctgcccaagc cgaccctagg cgggggtacc ggcccgccac
     6901 atctccccga tgcgcgggat ggtggagggc tcatgaggat ctaccgaccg aaggcccggc
     6961 tacttcggga cctcatcgaa gaactccttg acgtatgtca agtacctcgg tggccgggtc
     7021 gtcccgagtt tccgcaggtc gggccgccca cagcgatacc gtccctggcc taggcggaga
     7081 tcctcagggg atcagccgta caaactgtgc cacccgccac ggccgcggcg tagacgcccg
     7141 cgcacggata caagcctccg cccgccacgt cgagccggtt gtgcttggca agcgaacgca
     7201 ggattgctgc gtcgtggggc aagcctggct gcgcggcggt cgtcatgacg cagcggggat
     7261 cgggcaacat gaccgcgagt tggacctcgc tacccacccc gatcatctgg ccgagccatt
     7321 cattttcgac gaagcctgcg cccgccgtat cgacgaacag gttcatacga aagcgccgaa
     7381 tgtcgagcag actctctggt agcaattcgc tcaaccgatc caacgtggag gtcgtaataa
     7441 cgctgacggg gaagagatcc ataaacgaac cctctggcac cagcgacgcc atgccgagct
     7501 gactgaacag cgccgcaccg agcttagtat ccgtcacctc gtcgcggtgg ccttcagggt
     7561 cgagattctc gatgtccggg tggtactggt cgatcgtgaa atcgcttggc gcactgcggg
     7621 cgagcttgac ctcgcggcca aagaaccgcg atagaacggc atcgacatca tcggcatcgc
     7681 tcatgacgga ggtaccgtcg gtgagatcga tccgcaccga cggaagcttt tcgcccaagc
     7741 gcggcggctc tgaaaatgtg gcccggcatg aaagcagctc gggccacaac ttcgggtgtt
     7801 tagcgctcgc cactttgccc gtctcccgat cgatcactgc atatgcgcgg tcgccgagga
     7861 ttcccgcctc gcccactatg gcttgatcca gtcgctcgcc ttgcatcgat ttcaccggaa
     7921 agcgccacag ctctgagatc gtgccgatca cctcgctcgg ttccatgatc agctgctcct
     7981 ctgcgaatcc tggttgagtc attattcaac gcgacagtcg gtcatcagct ggttaccgca
     8041 gaagtggcga tgcgcgactg aaatcgcgct gattacgtcg cggtcaccca ccgggcagcc
     8101 atcgcggttc gcccatttga tcagcgcgtc agccgatctg atggacttgt tcgggttgtc
     8161 gggtaatcaa gcacaaggcg taggggtggc ggctgtcgag gtgtactgcc ctgaacacac
     8221 ccctgaaatg acgaaccgcc cccaacctgt gtggcttgtg tttgcacatg agggcgtaaa
     8281 cgcgggtgta aaacttgaca gcgcagtact cgatcgacgc cctgccctgg cggcggcaag
     8341 aatcacttga gaatccgcca agacgttcgc tccaacctat gtctgacagg ccatcacggc
     8401 acgtcaggca tagagaggac gaactatgaa aacccagatc atcgcggcgc ttacgattgc
     8461 gaccgcggca gcgctgcccc tggcagccgt cgccaacgct tgcggcggcg gtggcggcga
     8521 cgttcccacc gcctacgact ttgtgtctcc gtcgggaaac atcgcttgca cgatatacgc
     8581 ggacggctca ggcgcgaatt gcgaagtcca ggaacacact tgggtgattc cggcttcgac
     8641 agagggcccg tacgacagac cgtgcaattt caatttcgga ggtctggcga tctacgtcag
     8701 ccagggcaag ccaggaaagc tgggctgcta cgaggggtcc ggcaagttca agtaccccga
     8761 ccccgaccag cagacgctcg actacggcca gacatactcc cgcggggcga tcacgtgcga
     8821 cagtgagccg tccggcgtga catgcaccga caccgtgacc aggcatttct tccgcgtctc
     8881 tcgcgagtcc tacgaggtgg ggtgaccgac ttggcggatt cagctaaacg acgccgctga
     8941 ccatgtggcc cgcgtgcatg gcctccagca ccgagcgcat cgcgtcggcg ctttgccgcc
     9001 aagagaattc gccgctgcgg gtctgagcct tggcgccgag ctgttcacgc aacacctggt
     9061 cggtcagcag gcgttcgagc tggtcgacca gttcgtggtg gttatcgacg agcaggccgg
     9121 tcaccccgtc cacgatcgaa tccgtcaagc cgcctgagga ccggtaaccg atcgtcggca
     9181 cggaatgctg tcctgcctcg gtgaccgcca gcgcccagcc ctctttgcgc gacggcagca
     9241 cctgtaccca agagtgctgc agcacacggt gtttggtctc gtcgtcgacg tggccgtgga
     9301 acgtcaccgc gtcggagatg cccagcagcg cgccatgctc gacgagccgt tcttcccacc
     9361 agccgccgcc gacgatgtcc agatgcaggt ctggcatccg cggtcgtagc gcggcgatcg
     9421 cctccagcgc gtcctcaatc tgcttgtgtg gcactagccg tga
//
LOCUS       DASRJA010000524        13091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_217207,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000524 DASRJA010000000
VERSION     DASRJA010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13091
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217207"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFC01_22875"
     CDS             <1..441
                     /locus_tag="VFC01_22875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82494.1"
                     /translation="RYRARIMQAAADWTSVLINMNKDSVEADLTKLHEGTVGQLNADF
                     DSTVEPYRALVQKLQAKTTGQVDSVAVESVHHAPPGPNSAPKPQQQPELAASASRTDT
                     VMVVATSISENAGGDKPQTVRWNLRLDVSDVDGKLMISRLEPIR"
     gene            438..1127
                     /locus_tag="VFC01_22880"
     CDS             438..1127
                     /locus_tag="VFC01_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82495.1"
                     /translation="MRNRWRVLAFDILAPLAAVIGLVYIGVALAWPLWWVSVCSVLCL
                     LTVEGVIVNVVLARRDSVTVGTDDDGPGLRLAVVAVATIALVAGVIVGYLRWTVPERT
                     LNNDTTEVVGIASSVSEASATFTPQSPTASIDRVTAMMSPKSAEAYKNEFGKVATDLT
                     SRNISAQASTVSAGVEAIGPDAASVAVILHATQNAPGKPTDTAVLALRIQLSKAEGRW
                     LVDDVAPIHSR"
     gene            complement(1124..1522)
                     /locus_tag="VFC01_22885"
     CDS             complement(1124..1522)
                     /locus_tag="VFC01_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZN82496.1"
                     /translation="MADIAQILRTTRTVAIVGASDNPLRPSYGVWQYLKSASHYELYL
                     VNPTISEVDGTPVFPSLDEVPVVPDLVDVFRRHEYLPSVLDDTIKVGAKTLWLQQGLR
                     HEQVARDGKAAGLQVVMDRCLKVDYARLLG"
     gene            complement(1531..2052)
                     /locus_tag="VFC01_22890"
     CDS             complement(1531..2052)
                     /locus_tag="VFC01_22890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZN82497.1"
                     /translation="MTVAPDIRDGRRDAVAGPRRAAAPTQGRRPPIDERPVEFWPTSA
                     IRSALETDDLTVWQRIVVAIKRDPYGRTARQVEEVLETARPYGVSKALSEVLTRTREH
                     MEANERAEVARHVQSLLERSGLGQDEFASRIGVPSKDFAAYVTGETSPPASLMIRMRR
                     LSDRFAKMRNQHH"
     gene            complement(2049..4973)
                     /locus_tag="VFC01_22895"
     CDS             complement(2049..4973)
                     /locus_tag="VFC01_22895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN82498.1"
                     /translation="MMRLSSNLRRYRWAVFAVWLLLLVPSIYLAMNQSGNLTGGGFEV
                     EGSQSLHVQRELEQHFPDQGASPLALVAAPRADASFADMTAAVSQLERIAAEVPSVKL
                     VPNPQQPPPQPDRPYVVSLQPDFNNTGVVDVAKQLRQKIGINSDQPGETENGKVKLYV
                     IGQGALGAAATLATKHDIAQAEQWNLPIVLIVLLAVFGSLAAAAMPLVLGICTVAVTM
                     GLVYLLSMYTTMSVFVTSTVSMFGIALAIDYSLFILMRFREELRAGRDPRQAADAAMA
                     TSGLAVVLSGLTVIASVTGIYLINTPVLRSMATGAILAVAVAVLTSTTLTPAVLATFG
                     KAAAKRSSYLHWSRRAETTQSRFWSRWTGWVMRRPWVSALAASAFLLTLAAPAFSMVL
                     GNSMQRQFDPTHEIRGGVNAAAEALGPGALGPVRVLVTFPDGNAASAANKQPALDAVR
                     QKMAQGPNVVTVSPPVFGDDYRRALLSAVLSVDPEDMGARDTVDWMREQLPDTPGPNA
                     RIDVGGPTALIKDFDDRVSVTQPLVFGFVALIAFVMLLISIRSVFLAFKGVLMTVLSV
                     AAAYGSLVAVFQWGWLEELGFKPISSLDSTIPPLVLAMTFGLSMDYEIFLLTRIRERF
                     LQTKNTRDAVAYGVSTSARTITSAALIMIAVFIGFAFAGMPLVAQLGVACAVAIAVDA
                     TVVRLVLVPALMAMFDEWNWWLPRWLARILPSVDFEKPIPKVDMPDLVIIPDDISSLA
                     PSGADMRMVVKSAAKLKNLAPSTITVADPLALGCPSAREPVAAVANGHTNGRAHRNGA
                     TNGSTNGAVKACPPSLPGLHPVTIWRDRLSVALDALATEADSERAPVERQSPVETTNV
                     QLPTGDRLQIPTGAETLRMKSYLVMCRNTTRDFAEFAELVDSMETHTAAVVLSSMDRY
                     YCGDRSRRQWVATQLVRRLADPQPLDEHDTRMSGPEAEADWAKVRERCLSVAVAMLEE
                     AR"
     gene            5289..5846
                     /locus_tag="VFC01_22900"
     CDS             5289..5846
                     /locus_tag="VFC01_22900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemophore"
                     /protein_id="HZN82499.1"
                     /translation="MISSARSSTALRRSLYAVFAASAAGGAAVAALAVPSATAATDPC
                     AASEVARTIGSVATSTGNYLDTHPQTNQALTTISQQQAGPQSLGAIKSYFDANPQVAK
                     DMQQLQQPLVNLSTRCKLPVSLPQLMNLMQAAQQQGGAGLPGALQTAQNVGLPSAGSP
                     GSPSPASATTQGTGPLPGPASAAGR"
     gene            6019..6399
                     /locus_tag="VFC01_22905"
     CDS             6019..6399
                     /locus_tag="VFC01_22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZN82500.1"
                     /translation="MLLSARTARRAVAGTVGAGAIAGAMLFGALPSAMADDPANNPPN
                     CTAADLAGVSSGVSAATSAYLFTHPDVNWFFTSLEGQPREDIRDDVRTYLDANPVVKA
                     DLTGIRQPLVDLKNRCGTATPDVP"
     gene            6456..7124
                     /locus_tag="VFC01_22910"
     CDS             6456..7124
                     /locus_tag="VFC01_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN82501.1"
                     /translation="MVDDDPDVRTSVARGLRHSGFDVRVAANGKEALRLLSNESHDAL
                     VLDVQMPELDGVAVVTALRALGNDIPICVLSARDTVNDRIAGLEAGADDYLTKPFDLG
                     ELVARLHALLRRASHSDQTSDTMTVGPLTIDTARRLVFVDGERVDLTKREFDLLAVLA
                     ENTGVVLSRQRLLELVWGYDFDVDTNVADVFISYLRRKLERDGLPRVIHTVRGIGYVL
                     RDEP"
     gene            7163..8515
                     /locus_tag="VFC01_22915"
     CDS             7163..8515
                     /locus_tag="VFC01_22915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN82502.1"
                     /translation="MTLPRFLRSASLRTRVAIASAAAAAAVVAVFTILTSVVLANNDA
                     AQLDRRLDSIVDASINPTELQDPRRGVLTTGRSKSTGQVMFQRGLQLPPLPLGSANVE
                     VNGVEYRVRTVAVDQQGGVLMSVGIRADSILLNRARIPLYTAVGVVTVLIAAGLGWLL
                     AGPAIRPLRRLTEHTKRLGEGADAMPEVRGVREAEDLSEAMGEMLSRLAAAQRVTTNS
                     LQAAQDFAANAAHELRTPLTAMRADLDTLRIHDLPAEERAEVVQDLSRAQRRVEAIIT
                     ALGQLASGQLAQAEDRELIDVTDMLDRVAREIMRTAGGVEIVVEAADDLGSIWGWPGG
                     LRLAVDNLVRNAVTHGQATRIVLAARRRHDTMTIVVDDNGRGLPEEEHQTVLGRFSRG
                     SNAAPGGSGLGLALVAQQAALHGGCIELSDGPLGGLRARLTISTSPEPPTNSDLPASE
                     "
     gene            complement(8498..8878)
                     /locus_tag="VFC01_22920"
     CDS             complement(8498..8878)
                     /locus_tag="VFC01_22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HZN82503.1"
                     /translation="MPVVDTRRRVRRALIALVADIVCVIVFCTIGRRSHAEGLTVSGV
                     AHTAWPFLAGAAVGWLLIGGWRKPFTVIPTGVVVWVCTVAVGMLLRKATSAGVSTSFV
                     VVASLSTAVLLLGWRAAAALFRRG"
     gene            complement(8865..10073)
                     /locus_tag="VFC01_22925"
     CDS             complement(8865..10073)
                     /locus_tag="VFC01_22925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZN82504.1"
                     /translation="MRRTCLVLIVIAALTAACTPPTETPSAPTTSTTPPAEPTAVTPV
                     LASVIAAPIPVPATDGKVHLAYELQLTNALNQELTLTSVDVRGGDKSLLNLSGDRLAY
                     WTRVLGNTTPTTKIGPGQSAAVWLDVVLDQGAAVPAALRHEIGLSMAQPQPPLFPATI
                     TETVAPVGVQTRKPATIAPPLVGANWVDANSCCDMTPHRMALNPLNGQLWAAERFAID
                     YVQLLPDRRLFNGDKTKPESYPGFGANIHAVADGPVVGVLDGLPEQVPGASPSGLPLE
                     QYAGNHIVQDLGDGNYALYAHLKTGSISVKVGDRLSSGQVIALLGNSGNTDGPHLHFH
                     VMSTPDPLRSNGLPFVLSSFRLDGRITSMDAQDAVEAGQPAPMQPGFTPRDESAVSPL
                     VLDVMSYASR"
     gene            complement(10081..11211)
                     /locus_tag="VFC01_22930"
     CDS             complement(10081..11211)
                     /locus_tag="VFC01_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HZN82505.1"
                     /translation="MSHDAPASDARSQERPTKGKYWWLRWLILGVAAVVLAVELTLVW
                     DQLAKAWKSVYSANWWWVLAAVVAAMSSMHSFAQIQRTLLRSAGVTVKQWRSEAAFYA
                     GNALSTTMPGGPVLSATFIYRQQRIWGATPVVASWQLVMSGALQVVGLALLGLGGAFM
                     LGASKNPLSLIFSLGGFIALILLAQAVATRPELIDGIGVRLLSWFNSLRGKPADTGLD
                     KWRETLAQLESVSLSRRELGVAFGWSLFNWIADVGCLLAACYATGAHPSLAGVTVAYA
                     AARAVGSIPLMPGGLLVVEAVLVPGLVSSGMTLASAISAMLIYRLVSWIFISAIGWVV
                     FFFMFRTEKDIDPDATVDDDDVEQPPQFSPDPEVRHEPEPEP"
     gene            11322..12491
                     /locus_tag="VFC01_22935"
     CDS             11322..12491
                     /locus_tag="VFC01_22935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZN82506.1"
                     /translation="MPAGPSFDSVADESVTPLVRKTAAWAWRLLVIGTAVVALLWAVK
                     KLELIVVPVALATLLAALLLPIVDFLDRRGAPRGGAVALVLLGGIAVVGGILAFVVTQ
                     FIEGAPALVEQVSTSIKGVSDWLTRGPLHVNQEQIESARKSAIEALQNNQEKLTSGAL
                     STAGTVTEIVTGALLVLFTLIFVLHGGRHIYGFVTKLFPQDVRERVRDAGRAGFHSLI
                     GYVRATFLVALVDAVGIGTGLAIMGIPLALPLASLVFLGAFIPLVGAVVAGFFAVIVA
                     LIAKGWIYALITLGLIIAVQQLEGHVLQPLVMGRAVSIHPLAVVLAIAGGGVLAGIVG
                     ALLAVPTVAFLNSAVRVLLAREPAAEEAALEAQSGPVVKGEADDIDEGAVEAAKD"
     gene            complement(12488..>13091)
                     /locus_tag="VFC01_22940"
     CDS             complement(12488..>13091)
                     /locus_tag="VFC01_22940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82507.1"
                     /translation="TGTTRIPTARPQAASSDEPQTTRLSMAKNAVRNVVNNATGATQR
                     RPAPPPPAGPREEREIESWLGELRGSAPAGGQPAPPLPSNGPTREQGTGGPARPSADA
                     TRAMPDQRQRPPRPQPGNEPTTAIPAQGPPADGRGRPRPQNADATTAIPTLGTAPEGR
                     GLKQDAEATTEKLNTREDDERKRRGGGLSAQDLLRREGRY"
BASE COUNT         2207 a         4328 c         4366 g         2190 t
ORIGIN      
        1 cgctatcgcg cccgcatcat gcaggccgcc gccgactgga ccagcgtcct gatcaacatg
       61 aacaaggaca gtgtcgaagc cgacctgacc aaactgcacg agggcacggt cggtcagctc
      121 aacgccgact tcgactcgac cgtcgagccg tatcgcgcgc tggtgcagaa gctgcaagcg
      181 aaaaccaccg gccaggtcga ctccgtggcg gtcgaatccg tccaccacgc gccgcccggc
      241 ccgaatagtg cgccgaaacc ccaacagcag cccgagctgg cggcgtccgc gtcgcgcacc
      301 gacaccgtga tggtggtggc gacgtcgatc agtgagaacg cgggcggcga caaaccgcag
      361 accgtgcggt ggaacctgcg gctcgacgtc tccgacgtcg acggcaagct gatgatctct
      421 cggctggagc cgatccgatg agaaaccgtt ggcgcgtact ggctttcgac atcctggccc
      481 cgctagccgc cgtcatcgga ctggtgtaca tcggcgtcgc tctggcctgg ccgctgtggt
      541 gggtatctgt ctgttcggtg ctatgtctgc tgactgtcga gggcgtgatc gtcaacgtcg
      601 ttctcgcgcg ccgtgattcg gtcaccgtcg gcaccgacga cgacgggcca ggacttcggc
      661 tggctgtcgt cgccgtcgcg accatcgcac tggtcgccgg cgtgatcgtc ggctatctgc
      721 ggtggacggt gcccgagcgc acgctgaaca acgacaccac cgaggtggtc ggcatcgcca
      781 gcagcgtctc ggaagcctcc gcgacgttca cgccgcaaag ccccaccgct tcgatcgacc
      841 gagtcaccgc gatgatgtca ccgaagagtg ccgaggccta taagaacgaa ttcggcaagg
      901 tggcaacgga tttgacgagc aggaacatct cggcgcaggc cagcacggtc tccgcgggcg
      961 tcgaggccat cgggccggac gccgccagcg ttgcggtgat cttgcacgca acgcagaacg
     1021 cacccggcaa gccgaccgac accgcggtcc tcgcactacg tattcaactg tccaaggccg
     1081 aaggccgctg gctagtcgac gatgtggcgc cgatccactc gcgctaaccg agcaagcggg
     1141 cgtagtcgac cttcagacag cggtccatca ccacttgcag accggccgcc ttgccgtcac
     1201 gggccacttg ctcgtgccgc aacccctgct gcagccatag cgtcttcgcg ccgaccttga
     1261 tggtgtcgtc gagcaccgac ggcagatact cgtgacggcg gaacacgtcg accaaatcag
     1321 ggacgacggg aacctcatca agcgatggga ataccggcgt cccgtcgacc tcgctgatcg
     1381 tcgggttcac cagatacagc tcgtagtggc ttgccgactt caggtattgc cagacgccgt
     1441 agctcggacg cagtgggtta tcggacgcgc cgacgattgc gactgttcgg gtcgtacgca
     1501 ggatctgcgc gatgtccgcc acatcttctt ctagtggtgt tggttgcgca tcttcgcgaa
     1561 ccggtcggag agccggcgca tccgaatcat cagcgacgca ggcggactcg tctcgccggt
     1621 gacgtacgcc gcgaaatcct tgctaggcac accgatgcgg gacgcgaatt cgtcctggcc
     1681 cagacccgac cgctccagca gcgactgcac gtggcgggcc acctcggcgc gctcgttggc
     1741 ctccatgtgc tcgcgggtgc gggtgagcac ctccgagagc gccttggaaa ccccgtatgg
     1801 ccgtgcggtt tccagtactt cctcgacctg ccgcgcggtc ctgccgtacg gatcacgctt
     1861 gatcgcaacg acgatgcgtt gccagacggt caggtcgtcc gtctccaacg ccgaccggat
     1921 cgcggaggtc ggccaaaact cgaccggcct ttcgtcgatc ggcggccggc gcccctgcgt
     1981 tggcgctgcg gctcgcctcg ggccggcgac cgcgtcgcgg cgcccatccc gaatgtcggg
     2041 ggccaccgtc acctcgcctc ctcaagcatc gctaccgcca ccgataggca ccgctccctg
     2101 accttggccc aatcagcctc cgcctcgggg cccgacatcc tggtgtcgtg ctcgtccaac
     2161 ggttgcggat cggccaggcg gcgcaccaac tgggtggcga cccattgtct gcgtgagcgg
     2221 tctccacagt aataccggtc cattgacgac agcaccaccg ccgcagtgtg agtttccatc
     2281 gagtcgacca attcggcaaa ttcagcgaaa tccctggtcg tgtttcggca catcacgagg
     2341 tagctcttca tccgcagagt ctcggcgccg gtggggatct gcagccgatc acccgtcggc
     2401 agctgcacgt tggtggtctc cactgggctt tgccgttcca ccggtgcccg ctcagagtcg
     2461 gcctcagtcg cgagcgcgtc cagcgcgact gagagtcggt cccgccagat agttaccggg
     2521 tgcaggcccg gcaggctagg cgggcaagct ttgactgctc cgttcgtcga gccgttcgtc
     2581 gcgccgttgc ggtgcgcccg gccgttggtg tggccgttcg cgactgcggc taccggctca
     2641 cgggcagatg ggcaaccgag tgccaacggg tcagcaacgg taatcgtaga cggcgcaagg
     2701 tttttcagct tcgccgcgga cttgaccacc atgcgcatat cggcgccgga aggcgccagc
     2761 gacgagatgt catccgggat gatgaccagg tcgggcatgt cgaccttcgg tatcggcttc
     2821 tcgaagtcga ccgacggcag gatgcgggcc agccaacgcg gcagccacca gttccattcg
     2881 tcgaacatcg ccatcagcgc gggcaccaag accagccgca ccacggtggc gtcgaccgcg
     2941 atcgcgaccg cgcacgccac acccagctgg gccaccaacg gcatgcccgc gaacgcgaac
     3001 ccgatgaaca ccgcgatcat gatcagcgcg gcgctggtga tcgtgcgggc gctggtgctc
     3061 accccatagg cgacggcgtc gcgggtgttc ttggtctgca ggaaccgctc gcggatgcgg
     3121 gtcagcagga agatctcgta atccatcgac aggccgaacg tcatcgccag caccagcggc
     3181 ggaatggtgc tgtccagcga cgagatcggc ttgaagccca gctcttcgag ccagccccat
     3241 tggaagacgg cgacgaggct gccgtacgcg gcggccaccg acaggaccgt catcaggacg
     3301 cccttgaacg ccaggaacac cgatcggatc gagatcagca gcatcacgaa ggcgatcagc
     3361 gcgacgaatc cgaagaccag gggctgggtc accgagaccc ggtcgtcgaa gtctttgatc
     3421 aatgccgtcg ggccaccgac atcgatacgc gcgttgggcc caggtgtgtc cggcagctgc
     3481 tcgcgcatcc agtcaacggt gtcgcgggcg cccatgtcct cgggatcaac cgagagcact
     3541 gccgacagca gtgcccgccg gtagtcgtcg ccgaagaccg gcggcgacac cgtcacgacg
     3601 ttgggtccct gggccatctt ctggcgcacg gcgtcgagcg ccggctgctt attggccgcc
     3661 gaggcggcgt tgccgtcggg gaacgtgacc agcacgcgta ccggcccgag cgcccccggg
     3721 ccaagggctt cggccgctgc gttgacgccg ccacggatct cgtgggtcgg gtcgaactga
     3781 cgctgcatgc tgttgcccag gaccatggag aatgccgggg cggccagcgt cagcaggaag
     3841 gcggacgccg ccagcgccga cacccacggc cgtcgcatca cccaaccggt ccaccggctc
     3901 cagaaccgtg actgggtggt ctcggcgcgg cgcgaccagt gcaggtatga cgaccgcttg
     3961 gccgccgctt taccgaatgt cgccagcacc gcgggcgtca acgtcgtcga cgtcagcacc
     4021 gccacggcga cggcgaggat tgcaccggtt gccatcgacc tcagcaccgg ggtgttgatc
     4081 agatagatgc ccgtgacgga ggcgatgacc gtcagaccgg acagcaccac ggccaggcca
     4141 gacgtcgcca tcgccgcatc cgctgcctgc cggggatcgc ggcccgcgcg cagttcctcg
     4201 cggaaccgca taagaataaa tagtgaatag tcgatggcca atgcgatgcc gaacatcgac
     4261 accgtcgacg tgacgaacac cgacatcgtg gtgtacatcg acagcagata gaccagtccc
     4321 atggtcaccg cgacggtgca gatgccgagc accagcggca tcgcggcggc agccagcgag
     4381 ccgaacaccg cgagcaaaac aatcaggacg atcggaaggt tccactgctc ggcctgagcg
     4441 atgtcgtgct tggtggccaa ggtcgcggcc gcaccgagcg ccccctgacc gatgacgtaa
     4501 agcttgacct tcccgttctc ggtctcgccg ggctgatcgc tgttgatgcc gatcttctgc
     4561 cgcaactgct tggcgacgtc gaccacgccg gtgttgttga agtcaggctg cagggagacg
     4621 acgtaaggac ggtccggctg gggcggcggc tgctgcgggt tcggcaccaa cttgacgctc
     4681 ggcacctcgg ccgcgatgcg ttcgagctgg ctgaccgcag cagtcatgtc tgcgaaggaa
     4741 gcgtccgcgc gcggggcggc gaccagggcc aacggcgagg cgccctggtc ggggaagtgc
     4801 tgctcgagct cacgctgcac gtgcagcgac tgggaaccct cgacttcgaa gccgccgccg
     4861 gtcaggttgc cggactggtt catcgccaga tagatcgagg ggaccaaaag aagcagccaa
     4921 acagcgaaca ccgcccagcg atatctgcgc aggttgctgc tcaagcgcat catgaactgc
     4981 tggatggcaa actccccgct ttctgcccgg cgttgcgaag ctgagcgtac aacagcgtcg
     5041 ggtagggtga cgggccctga gcaacgcctt aaccagctct aactccactc tcgtgattcg
     5101 ccggcgaagt gttacgcgca gctgcggatt ttcctgacgt tcacctgcat gttcattcgc
     5161 tggattggca gcggcagtgg cgccgatggc aatatgtgcc gaggactcca gggtcgggga
     5221 acccaaccgt ccgatcgcag gcgtttgcgg aagcgtcaaa agcgttcgca aaccgtaagg
     5281 agttgtgcat gatttcgagc gcccgatcga gcactgcgct tcgccgcagc ctctacgcgg
     5341 tgttcgctgc gtcggcggcc ggtggcgccg cggtggcagc gctggcagtg ccctcggcaa
     5401 ccgcagccac ggatccctgt gccgcgagcg aggtcgccag gaccatcggc tcggtggcca
     5461 cctcgaccgg caactacctc gacacacacc cccaaaccaa ccaggcgttg acgacgatct
     5521 cgcagcagca ggccggcccg cagtcgctgg gcgccatcaa gtcgtacttc gacgccaacc
     5581 cacaagtcgc caaggacatg cagcagttgc agcagccgct ggtgaacctg tccacccggt
     5641 gcaagctgcc ggttagcctg ccccagctga tgaatctgat gcaggccgcg caacaacagg
     5701 gcggcgcggg tttgccggga gcgctgcaga ccgcgcagaa cgtagggctt ccaagcgcgg
     5761 gatcgccggg ttcgccgtcg cctgcgtcgg ccaccacgca gggcaccgga ccacttcccg
     5821 gcccggcatc ggccgccggc cgctaatcca ggacgacgcg cggagcgtcg caggtggggg
     5881 tgctgagctg cggtttcaga ctttcttacg attggttaag cgcaaggcaa gaaagtcggg
     5941 accctttctg cttagctctc ttattgtcgg aaccgccgat ggttgcggcc tctccacttc
     6001 atatgaagga gcctgtccat gttgctctcg gcccgtaccg cgcggcgtgc ggtagctggc
     6061 acggtcggcg ccggcgctat tgccggtgcg atgctgtttg gtgctctccc ctcggcgatg
     6121 gccgacgacc cggcgaacaa cccgccaaac tgcaccgctg cagacctggc tggcgtgtca
     6181 tctggtgtct cggcagcgac gtcggcgtac ctgttcacgc acccggacgt gaactggttc
     6241 ttcaccagcc tcgagggcca gccgcgcgag gatatccgtg acgacgtccg cacttacctg
     6301 gacgccaatc ctgtggtgaa ggccgatctc accgggatcc gccagccgct ggtcgacctg
     6361 aaaaaccgct gcggtacagc cactcctgac gttccgtaat tcgcgtgcgg gttggtgaac
     6421 cacccgcgca ggagagcgcg agccgcactg tgctgatggt cgacgacgac ccggacgtgc
     6481 gaacctccgt tgcccgcgga ttgcggcatt cgggtttcga cgttcgggtc gcggcgaacg
     6541 gtaaagaggc gctgcggctg ttgtccaacg agtcgcatga cgcgttggtg ctggatgtcc
     6601 agatgccgga actcgacggg gtcgcggtgg tcaccgcctt gcgggcgttg ggtaacgaca
     6661 tcccgatctg cgtgctttct gcgcgcgaca cggtcaatga ccggatcgcg gggttggagg
     6721 cgggcgccga cgactacctg accaagccct tcgatctggg cgagttggtg gcgcggctgc
     6781 acgccctgtt gcggcgggcg agccattcgg atcagacgtc ggacacgatg acggtcgggc
     6841 cgttgaccat cgacaccgcg cgccggctgg tgttcgtcga cggcgagcgt gtcgacctga
     6901 ccaagcgcga gttcgacctg ttggcggtgc tggccgagaa caccggcgtg gtgttgtcgc
     6961 ggcaacgact actcgaactg gtgtggggct acgacttcga cgtcgacacc aatgttgccg
     7021 acgtgttcat ctcctacctg aggcgaaagc tcgaacgcga cggcctgccc agggtgatcc
     7081 acaccgttcg cgggatcggg tacgtgctcc gagacgagcc gtaggcgagt cgcgcatcag
     7141 gcacagtgac gagtccgttg aaatgacctt gccgcggttc ctgcgatccg cctcattgcg
     7201 tacccgggtt gcgatcgcgt cggccgccgc cgcggctgcg gtggtcgccg tgttcaccat
     7261 cctcacctcg gtggtgctcg cgaataacga tgcggcgcaa ttggatcggc gactcgattc
     7321 cattgtcgac gccagcatca accccaccga actgcaggac ccccgccgcg gcgtgctcac
     7381 caccgggcgg tccaagtcca ccgggcaggt gatgttccag cgcggcctgc agttgccgcc
     7441 gctgccgttg ggcagcgcga acgtcgaagt gaatggtgtg gaataccggg tgcgcaccgt
     7501 tgccgtcgac cagcagggcg gcgtgctgat gtcggtcggc atccgcgccg acagtatctt
     7561 gttgaaccgc gccaggattc cgttgtacac ggctgtcggt gtggtcactg tgttgatcgc
     7621 cgccggcctt ggctggctgc tggcgggccc cgcgatccgt ccgctacgca ggttgaccga
     7681 gcacaccaaa cggctgggtg agggcgccga cgcgatgccc gaagtgcgtg gcgtccgcga
     7741 ggcggaggat ctgtcggagg cgatgggcga gatgctgagc aggctggccg ccgctcagcg
     7801 ggtgaccacg aactcgctgc aggccgcaca ggatttcgcc gccaacgccg cgcatgaact
     7861 gcgtacccca ctcacggcga tgcgagccga cctggacacc ctgcgaatcc acgatctgcc
     7921 cgccgaggag agagctgaag tggtgcagga cctttcgcgc gcacagcgcc gcgtggaggc
     7981 gatcatcacc gcgctcgggc agctcgcgtc cggccagctg gcgcaggccg aggaccgcga
     8041 actcatcgat gtcaccgaca tgctcgaccg ggtggcgcgc gagatcatgc gcaccgccgg
     8101 cggagtcgag atcgtggtcg aggccgcgga cgatctcggg tcgatctggg ggtggccggg
     8161 cgggctgcgg ctagccgtcg acaacctggt gcgcaacgcg gtgacgcatg ggcaggcgac
     8221 gcgaatcgtg ttggcagcgc gccgtcggca cgacacgatg acgatcgtcg tcgacgacaa
     8281 cggcaggggt cttccggagg aggagcatca aacggtgctt ggccgattct cccgcggcag
     8341 caatgcggcg ccgggcggct cggggctcgg cctggcactg gtagcgcagc aggccgcgtt
     8401 gcacggcggg tgcatcgagc tgtcggacgg gccattgggc gggttgcgcg cgaggttgac
     8461 tatctcgact tcgcctgagc cgccgaccaa ttcagactta cccgcgtcgg aatagtgcgg
     8521 ccgcggcgcg ccagcccagt agcagcaccg cggtggacag cgacgccacc accacaaagc
     8581 tcgtcgaaac ccccgctgaa gtcgccttgc gcaacagcat gcccaccgcg accgtgcaca
     8641 cccacaccac cactccggtg gggatgaccg tgaacggttt gcgccacccg ccgatcagca
     8701 gccagcccac cgcggcgccc gccagaaatg gccatgcggt gtgcgcgacc cctgacacag
     8761 tcaatccttc tgcgtggctt cggcgcccga tcgtgcagaa cacgatcacg cagacgatgt
     8821 ctgcgaccag agcgatcagc gcccgtcgca cccgtcggcg cgtgtcaacg actggcatag
     8881 ctcatcacat ccaaaaccaa tgggctgacg gcagattcat cacgcggggt gaagcccggc
     8941 tgcatcggcg cgggctgacc agcctcgact gcgtcttgag catccattga ggtgatgcgc
     9001 ccgtcgagcc gaaatgacga cagcacgaac ggcaggccgt tggaccgcag cggatctggg
     9061 gtgctcatga cgtggaaatg tagatgcggt ccgtcggtat tgccagagtt gccgagcagc
     9121 gcgatcacct gcccggaact cagccggtca ccgaccttga cgctgatact gccggtcttg
     9181 aggtgggcgt agagcgcgta gttgccgtcg ccgaggtcct gcacaatgtg gtttcccgca
     9241 tactgctcga gcggcagacc gctcgggctc gcgcccggaa cctgttcggg taatccgtcg
     9301 agcacaccga cgacggggcc gtcggccacc gcgtggatgt tggccccgaa tccggggtag
     9361 ctctccggtt ttgtcttgtc gccgttgaac aatcgcctgt cgggaagcag ctgcacgtag
     9421 tcgatggcga accgctcggc cgcccacagc tgcccgttga gtggattcag ggccatccgg
     9481 tgcggtgtca tatcgcaaca actgttggcg tccacccagt tcgctcccac aagcggtggt
     9541 gcgatggtcg ccggcttgcg ggtctgcacg ccgacgggtg cgacagtctc ggtgatcgtg
     9601 gcgggaaaca gcggcggctg tggctgggcc atcgagaggc cgatctcgtg cctaagggcg
     9661 gctggcaccg ccgcgccctg gtcgagcacg acgtccagcc acaccgccgc cgattggcca
     9721 ggtccgattt tcgttgtggg cgtggtgttt ccgagcactc gcgtccagta cgcgagccga
     9781 tcgcctgaca ggttcagcag ggacttgtcc ccgccgcgca cgtcgaccga cgtcagggtc
     9841 agctcctggt tgagcgcgtt ggtcagctgc agttcgtagg cgaggtggac tttgccgtcg
     9901 gtggcgggca ccggaatcgg tgcagcaatg acactggcca acaccggtgt caccgccgtg
     9961 ggctcggcgg gtggcgtcgt cgaagtcgtt ggtgctgaag gggtttcggt aggtggcgtg
    10021 cacgccgccg tcaacgcggc aatcactatg agcaccaggc acgtgcggcg catgagttcc
    10081 ctatggctcg ggttcgggtt cgtgccgcac ctcaggatcg gggctgaact gcggcggctg
    10141 ctcgacgtca tcatcgtcga cggtggcgtc ggggtcgatg tccttctcgg tgcggaacat
    10201 gaagaagaac accacccagc cgatcgcgga aatgaagatc cagctgacca accgatagat
    10261 gagcatcgcg gagatcgccg aggccagggt catgccggac gacaccaggc caggcaccag
    10321 caccgcctcc accaccagca gaccgcccgg catcaacggg atcgacccga ccgcgcgggc
    10381 cgccgcgtac gccacggtca cacccgccag agacggatgc gcgccggtgg cgtaacaagc
    10441 cgccagcagg cagcccacgt cggcgatcca gttgaacagc gaccagccga acgcgacgcc
    10501 gagttcgcgg cgggacaggc tgaccgactc cagttgcgcc agcgtctcgc gccacttgtc
    10561 cagacccgtg tcggccggct tgccgcgcag cgagttgaac cacgaaagca gccgtacccc
    10621 gatgccgtcg atgagttcgg gacgggtcgc caccgcttgc gccagcagga tcagggcgat
    10681 gaacccgccg agcgaaaaga taagcgacag tgggtttttg ctggcaccca acataaacgc
    10741 gccgcccaag ccgagcagcg ccagcccgac cacctgcagc gcgcccgaca tcaccagctg
    10801 ccacgacgcc accacaggtg tcgcgcccca gatccgttgt tggcggtaga tgaacgtggc
    10861 cgacagcacc gggccgcccg gcatcgtggt ggacagtgcg ttgcccgcgt agaacgccgc
    10921 ctccgagcgc cactgcttga ctgtgacgcc ggccgaccgc agcagcgtgc gctgaatctg
    10981 cgcgaagctg tgcatcgacg acatcgcggc cacgaccgcc gccagcaccc accaccagtt
    11041 cgccgagtag acgctcttcc acgccttggc caactgatcc cagaccagcg tcagttcgac
    11101 cgcgagcacg acggctgcga cgccgaggat cagccagcgc agccaccagt atttgccctt
    11161 ggtaggccgc tcctggctgc gggcgtcgct cgcaggcgcg tcgtgggaca cgccaatagg
    11221 gtaacggcgc agttcgtccg gatattgtcg ctggcgcagg cgcgccgaag tcgattcgcg
    11281 agattcccag cggggccgca gcctgccgtt acgctaccgg catgccagct ggcccttcct
    11341 ttgattccgt cgccgacgag tcggtgactc cgttagtccg caagacagcg gcgtgggcat
    11401 ggcggctgtt ggttatcggc acggccgtcg tggccctgct gtgggcggtg aagaagctcg
    11461 aactcatcgt ggtgcccgtt gcgttggcca cgctgctggc cgcactgctg ctgcccatcg
    11521 ttgacttcct ggaccggcgc ggcgctccac gcggcggtgc ggtggccctg gtgttgctcg
    11581 gcggcatcgc cgtcgtgggc ggcatcctcg ccttcgtcgt cacccagttc atcgagggcg
    11641 caccggcact cgtggagcag gtgagcacca gcatcaaagg cgtcagtgac tggctgacgc
    11701 gggggccact gcacgtcaac caggagcaga tcgagagcgc ccgcaaatcc gccatcgaag
    11761 cgctgcaaaa caaccaggag aaactgacca gcggggcgct gtcgacggcg ggcaccgtga
    11821 ccgagatcgt caccggcgcc ctgctggtgt tgttcacgct gatattcgtg ctgcacggcg
    11881 ggcgccacat ctacggattt gtcacgaagc tctttccgca ggacgtgcgg gagcgagttc
    11941 gtgacgcggg ccgggcgggt ttccactcgc tgatcggcta tgtgcgggcg acgttcctgg
    12001 tcgcgctcgt cgacgccgtc ggcatcggaa cgggtctggc gatcatgggt atcccgctgg
    12061 cgctgccgtt ggcgtcgctg gtgttcctcg gcgcgttcat cccgctcgtg ggtgcggtgg
    12121 tggccggctt cttcgccgtc atcgtggcgc tgatcgccaa ggggtggatc tatgcgttga
    12181 tcacgcttgg cttgatcatc gccgtgcaac aactggaagg ccatgtgcta cagccgctgg
    12241 tgatgggccg ggcggtgtcg attcacccgc tggcggtggt gctggccatc gcgggcggcg
    12301 gtgtgctggc cggtatcgtc ggggccctgc tggctgtgcc gacggtcgca ttcctcaaca
    12361 gcgcggtgcg cgtgctactg gccagagaac ctgcggccga agaggccgca ctggaggccc
    12421 aaagcggccc ggtcgtcaag ggcgaagccg acgacataga cgaaggggcc gtcgaggcag
    12481 cgaaggatta gtaccggccc tcgcgccgca gcagatcctg cgcgcttaac ccgccgccgc
    12541 ggcgcttgcg ctcgtcgtct tcgcgggtgt tgagcttctc ggtcgtcgcc tcggcgtcct
    12601 gtttgagtcc cctgccctca ggcgcggtcc ccagtgtcgg gatggctgtc gtcgcatcgg
    12661 cgttctgcgg ccgcggacgt cccctaccat cggctggggg cccttgcgcg gggatcgcgg
    12721 tggtcggctc gttgcccggc tgcggacggg gcggccgctg gcgttgatcc ggcatcgcac
    12781 gcgtggcgtc cgctgatggc cgcgctgggc ccccagtccc ctgctcgcgc gtgggcccat
    12841 tcgaggggag cggcggtgcc ggttggccac cggccggtgc gctgccgcgc aattcgccca
    12901 accacgattc gatttcgcgc tcctcgcgtg gcccggcggg gggaggaggc gcgggccggc
    12961 gctgcgtcgc cccggtcgca ttgttgacga cgttgcgaac agcgttcttg gccatcgaca
    13021 gccgggtggt ctgcggctcg tcagaagacg cagcctgcgg gcgcgcggtc gggatccgcg
    13081 tcgtcccggt a
//
LOCUS       DASRJA010000525        10106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_146999,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000525 DASRJA010000000
VERSION     DASRJA010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10106
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146999"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFC01_22945"
     CDS             <1..436
                     /locus_tag="VFC01_22945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN82508.1"
                     /translation="LGLIRSGLDSGARLVTGGGIPENLPTGYYVQPTLLADVDPDSQV
                     AQEEIFGPVLTVTPYDTDDEAVAIANNTIYGLSGEVSSGDVDRAFEVAKRMRTGNVTI
                     NGKSHFGIGSPFGGTKQSGLGYRNGEEGYKAYLEAKTIGMPD"
     gene            436..858
                     /locus_tag="VFC01_22950"
     CDS             436..858
                     /locus_tag="VFC01_22950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN82509.1"
                     /translation="MDEAAIAALLYRYARAVDTKDWELYRSVFTDDAYIDYSSAGAAT
                     GGRDEVVEWFEANFGVIPWSMHYITNIESDIDGDTAKVRAMFYNPMQLPGMTEPSYCG
                     GYYHHELVRTPSGWRSRHLREENVWFVNSPAGAPEPHM"
     gene            920..1238
                     /locus_tag="VFC01_22955"
                     /pseudo
     CDS             920..1238
                     /locus_tag="VFC01_22955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1293..2390)
                     /gene="rlmN"
                     /locus_tag="VFC01_22960"
     CDS             complement(1293..2390)
                     /gene="rlmN"
                     /locus_tag="VFC01_22960"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZN82510.1"
                     /translation="MVTSLPLVFDAPRRAMPPRHLADLDEDARAAAVAELGLPAFRGK
                     QLANQYYGRLIADPQQMTDLPSAVREQIADALFPRLLDAVREIECDAGETRKMLWRAV
                     DGTTFESVLMRYPQRNTACISSQAGCGMACPFCATGQGGLTRNLSTAEILEQVRAAAV
                     ELRDRDHGRLSNIVFMGMGEPLANYNRVLAAVRRTTAAPPHGFGISARSVTVSTVGLA
                     PAIRKLADERLGVTLALSLHAPDDELRDTLVPVNNRWKVSEALDAARYYADVTGRRVS
                     IEYALIRDINDQPWRADLLGKRLHGALGPLVHVNVIPLNPTPGSEWDASPKAAEREFV
                     RRVRERGVSCTVRDTRGREIAAACGQLAAEG"
     gene            2484..3440
                     /locus_tag="VFC01_22965"
     CDS             2484..3440
                     /locus_tag="VFC01_22965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN82511.1"
                     /translation="MPLGAASALTFFYALGYPIGALAVSVMSPMAVLVFRFTLAAAIL
                     SGWALFARVTWPTGRTLVHVLISGLLAQGLLFTCLYVALMHGAPAVLGAVVISMNPVV
                     TALLAAMFLGEGLTWMRVGALVLGVLAVLAACAGRLMMVGGVDPAVLLLLVSLFSVAA
                     GGVYQQRFCSKVDFRATAALQNAVCIAPVVALAAIMPFTVTDPWKAAGAVAAVVLLNA
                     VLSMTLYVRGVNEYGAGAVAMLFAVIPAVAGLLSWLMLGQRPDIGIAIGLVVGAAACS
                     LARAGIPRLNARVSRQQRQHDPAGDGRGQHRVDPVQDSAVPR"
     gene            complement(3351..4235)
                     /locus_tag="VFC01_22970"
     CDS             complement(3351..4235)
                     /locus_tag="VFC01_22970"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZN82512.1"
                     /translation="MTEQRATPVADTEAGPTEPPKKASRAGRDLPAAIAVGVLLGAMA
                     IGVLLFAPIGWLPVLAVAMPIATHEVVRRLREAGYALPLIPLLIGGQAMIWLTWPYGP
                     AGLLGAYGGTIVVCMVWRLVGHGLSEQPVNYLRDISATVLLATWVPLFASFSALLIFQ
                     DRGGIRTFTVIATVVFADIGGYVAGVLFGKHLMVPAISPKKSWEGLGGSLLFGITAAV
                     LSVAFLMDKPAWVGVPLGLMLVITGVLGDLVESQVKRDLGIKDMGTLLPGHGGILDRI
                     DAMLPSAVAGWIVLTLLA"
     gene            complement(4235..4792)
                     /gene="frr"
                     /locus_tag="VFC01_22975"
     CDS             complement(4235..4792)
                     /gene="frr"
                     /locus_tag="VFC01_22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZN82513.1"
                     /translation="MIDETLFDAEEKMEKAVSVARDDLSSIRTGRANPGMFSRINVDY
                     YGSPTPITQLSSINVPEARLVVIKPYEQSQLKPIEDSIRNSDLGVNPSNDGNVIRIAI
                     PQLTEERRRELVKQAKSKGEDARVSVRNIRRKAMEELHRIKKDGEAGEDEVSRAEKDL
                     DKTTHTYTAQIDELVKHKEGELLEV"
     gene            complement(4805..5554)
                     /gene="pyrH"
                     /locus_tag="VFC01_22980"
     CDS             complement(4805..5554)
                     /gene="pyrH"
                     /locus_tag="VFC01_22980"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZN82514.1"
                     /translation="MAEPTTTNGNEPPIRPMYSRVLLKLGGEMFGGGEVGLDPDVVHQ
                     VARQIAEVVRSGVQVAVVIGGGNFFRGAQLQQRGMERTRSDYMGMLGTVMNSLALQDF
                     LEKEGIATRVQTAITMGQVAEPYIPLRAVRHLEKGRVVIFGAGMGLPYFSTDTTAAQR
                     ALEIGAEVVLMAKAVDGVFTADPRIEPDAELLTAISHREVIDRGLKVADATAFSLCMD
                     NGMPILVFNLLVDGNIARAVAGEKIGTLVTT"
     gene            complement(5643..6323)
                     /locus_tag="VFC01_22985"
     CDS             complement(5643..6323)
                     /locus_tag="VFC01_22985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN82515.1"
                     /translation="MTQPMDQMFESAYKGEAEELGSGMKPPWSIGEPQPELAALIEQG
                     KFHGDVLDAGCGEAAISLHLAERGFTTVGLDQSPTAIDLARAEAAKRGLTTAAFDVAD
                     ISNFDGYDGRFGTIVDSTLFHSMPVELREGYQQSIVRAAAPGASYFVLVFDKAGMPDS
                     PANPVTKDELRDIVSKYWTIDEIRPARIHGVFPDGFEQFFPVTDVREEPKGRRSVPAF
                     LLTAHLPG"
     gene            6360..6800
                     /locus_tag="VFC01_22990"
     CDS             6360..6800
                     /locus_tag="VFC01_22990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN82516.1"
                     /translation="MLDMDEPFEDQPLGYLLSRAMFALKSDVTATVLDPLEVAFPEYL
                     CMRVLSKFPGQSNADLARALNVSPQAMNMVLRGLEDRGLVTRPTSVSSGRSLPAQLTR
                     DGEELLKRTDAGVQAAERRLMANLTDKERYDFKRILAALGSDPF"
     gene            6827..7786
                     /locus_tag="VFC01_22995"
     CDS             6827..7786
                     /locus_tag="VFC01_22995"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HZN82517.1"
                     /translation="MTGPIYVSRLLTDRAMAHLRSLDTPLRVGGEAPPSRAELESGIA
                     GAAAAVVTLTERVDADLLAAAGSQLKVVANVAVGYDNIDVDAAEAAGVIVTNTPGVLD
                     RATADHTFALILAASRRITEGDRLIRSREPWIWGPRMLVGLDVSAGATLGILGYGRIG
                     RAVARRAQAFDMAVVAMSRSREPGTTEDDVTFVDTATLLADSDVVTVLTPLTPETHHL
                     IDAEALTAMKSTAYLINTARGGIVDEAALIAALHAGEIRGAALDVFEDEPNVNPALLD
                     APNLVLTPHIASAGEATRDAMGILAIDNAAAVLAGNPPLTPVR"
     gene            complement(7791..9767)
                     /locus_tag="VFC01_23000"
     CDS             complement(7791..9767)
                     /locus_tag="VFC01_23000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HZN82518.1"
                     /translation="MTVESTVPSTEAIDNTARYPLDPLTGAEIETAATVITDSEYATP
                     TLKFVMIQLAEPDKTPGLTFGGVVDVPRRAFVSMYDGAAKLVYEAIVDIGARVIDSWT
                     AVPGRFPSYLVEHMTGVEAVVRADPRWQDAMRKRGVSDFDLAMIDPWPAGYYGSQDHY
                     DNSPLICRPLTFMRAAPSEHGYARPVEGLIVTFDLDAMKVIDIEDHGAVPLPPTAGNY
                     AEQFMFDENNRPAFSQFRDDVKPIEITQPDGPSFTVDGWKVQWQKWSMRVGFNPREGI
                     SLHEITYTDRGETRPIIYRASLSEMVVPYGDTEPTHWNKNVFDMGEVGMGFSANPLTL
                     GCDCLGEIYYFDGTVNDSAGNAVTIPNAICMHEEDYGISWKHTDFRTGEVEVRRARRL
                     VISMICTVGNYEYGFFWYFYNDASIEVEVKLSGVLTTGAVKDGEEPRWGKLVAPNIYG
                     PNHQHFFNFRLDMSVDGAGNSVYEVDSIPEPDPELNPHHNAWITRDTLVASEADGARD
                     WDWSKGRYWKITNPSKKNELGGPVAYKLTPREVVPVMVQEGSYIYDRARFVQHNLWVT
                     KYDPAEKFAAGDYMYQSSDMQGLPEYIADDAPLEDTDVVLWYTVGAHHVVRPEDWPVM
                     PCAYTGFHLKPVGFFDGNPALDIPPSPPAACNHH"
     gene            complement(9764..>10106)
                     /locus_tag="VFC01_23005"
     CDS             complement(9764..>10106)
                     /locus_tag="VFC01_23005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN82519.1"
                     /translation="GFFFFVPLVIARKKYADRYESEDFWRIPGGMPAVYAVAAIGIIA
                     TCAGVYYSFASPWIDVPAATWMTWVGSIAVGMFVLGAIVYFFGRRSAAKVTQEDALAH
                     LAVLELKDDAK"
BASE COUNT         1668 a         3318 c         3291 g         1829 t
ORIGIN      
        1 gctcggcctg atccgcagcg gcctcgactc gggcgcccgg ctcgtcaccg gaggcggcat
       61 tcccgagaac ctgccgaccg gttactacgt gcagccgacg ctgctggccg acgtcgaccc
      121 cgattcgcag gtcgcgcagg aggagatctt cggcccggtg ctgacggtga cgccgtatga
      181 cacggacgac gaggccgtcg cgatcgccaa caacaccatc tacgggctct ccggcgaggt
      241 cagcagcggc gatgtcgacc gggcgttcga ggtagccaag cgcatgcgca ccggcaatgt
      301 cacgatcaac gggaagagcc acttcggcat cggcagcccg ttcgggggca ccaagcagag
      361 cgggctgggc taccgcaacg gcgaggaagg ctacaaggcg tacctcgagg ccaagacgat
      421 cggcatgccg gactgatgga cgaggccgcg atcgccgccc tgctgtaccg ctacgcccgc
      481 gcggtcgaca ccaaggattg ggagctgtac cggtcggtgt tcaccgacga cgcctatatc
      541 gactattcgt cggcgggtgc tgccaccggc ggccgcgatg aggtcgtcga gtggttcgag
      601 gcgaatttcg gggtgatccc gtggagcatg cactacatca ccaacatcga gagcgacatc
      661 gacggcgaca cggcgaaggt gcgggcgatg ttctacaacc cgatgcaatt gccggggatg
      721 actgagccga gttactgcgg cggctactac caccacgaac tggtgcgcac acccagcgga
      781 tggcgcagcc gtcatctccg tgaggagaac gtgtggttcg tcaactcgcc ggccggggct
      841 ccggaaccgc atatgtgaat ttgccccatt agcgagcgac aactacggag ttgtcggacg
      901 tgcctgccac ggttcaacga tggaaaccag gcatctactg cagggaatcg agctccgcta
      961 tgcgctcatg atgtacctgt tgcaacacgg ccatacgaca gtcgacgaac tcatcaaggc
     1021 tcttgagtgg cagggcttcg agatccgccg gacgggcatc gaaacaagtg tcggatgcgc
     1081 tgcgctggga gcggcggcgc ggcagggttc gtcggcttgg gcgtggactt tacggccccg
     1141 ggtttgtacc gcgcggcacg gagtaccgga tttatattcg ggtgatggcc ttgcgggccg
     1201 cggcgaagcc cctgtggttc cacggtgtcc ggctataggt tccgatgtcg ctcgctacgc
     1261 gggcactttc cactatgtcc ccagcgcctg cgtcagccct cggccgccaa ttgcccacag
     1321 gccgccgcaa tttcgcggcc ccgggtgtcg cgcaccgtgc atgacactcc ccgctccctc
     1381 acccgccgca cgaactctcg ctcagctgcc ttcgggctgg cgtcccattc gctgcccggc
     1441 gtggggttca gcgggatgac gttgacatgt accagcggcc ccagtgcacc gtgcagacgt
     1501 ttgcccaaaa ggtctgcgcg ccaaggctgg tcgttgatgt cgcggatcaa cgcatattcg
     1561 atagatacgc gtcgcccggt aacgtcggcg taataccgtg ccgcgtcaag ggcttcggac
     1621 accttccacc gattgttgac cgggaccagc gtgtcgcgca gttcatcgtc gggcgcatgc
     1681 agcgacagcg ccagtgtgac accgagccgt tcgtccgcga gcttgcgaat cgccggagca
     1741 agccccaccg tcgacacggt caccgaccgc gccgaaatgc cgaacccgtg cggcggcgcc
     1801 gcggtggtgc gccgcaccgc ggccagcacg cggttgtaat tggccagcgg ttcgcccatg
     1861 cccatgaaca cgatgttcga caagcggccg tggtcgcggt cccgcagttc gaccgcggcc
     1921 gctcgcacct gctcgaggat ctccgccgtc gacaggttgc gggtcagccc gccctgcccc
     1981 gtcgcgcaga acggacacgc catcccacag cccgcctgcg acgagatgca cgccgtattg
     2041 cgctgcggat agcgcatcag caccgactcg aacgtcgtgc cgtcgaccgc ccgccacagc
     2101 atctttcgcg tctcgcccgc atcgcactcg atctcgcgta ccgcgtcgag cagacgcggg
     2161 aacaacgcat cggcgatctg ttcacggacc gcggacggca gatcggtcat ctgctgcgga
     2221 tcggcgatca gccggccgta gtactggttg gccagctgct tgccgcgaaa tgcaggcagc
     2281 ccgagctccg cgacggcggc ggcacgcgca tcctcgtcga ggtcggcgag gtggcgcggc
     2341 ggcatggcgc gtcgcggcgc atcaaacacc aggggcaggg atgtgaccat gagcaactgt
     2401 cagtatctca cccgtgcgtg gcggaaattg attggcggcg gcgggcgttg gtcgcaacta
     2461 tggagcgtat ggtcacgcgg acgatgcccc tcggcgccgc gtcggcgttg acgttcttct
     2521 acgcgctcgg ctatcccatt ggggcacttg cggtttcggt gatgtcgcca atggcggtcc
     2581 tggtgtttcg gttcactttg gccgcagcga ttctgtccgg ctgggcactg ttcgcccggg
     2641 tgacgtggcc cacaggtcgc acgctcgtgc atgtgctgat cagcgggctt ctcgctcagg
     2701 gcctgttgtt cacctgccta tatgtggcgc tgatgcacgg tgcccccgcg gtgttgggcg
     2761 cagtcgtcat ctcgatgaat cccgtggtca cggcgctgct ggccgccatg ttcctcggtg
     2821 agggtttgac gtggatgcgg gtcggcgcac tggtgctcgg cgtcctcgcg gtgctggccg
     2881 cgtgcgcagg ccggctgatg atggtcggcg gcgtcgaccc cgcggtcttg ctactgctcg
     2941 tctcgttgtt cagcgtcgca gcgggcggtg tctaccagca gaggttctgc tccaaggtgg
     3001 acttccgggc gacggcggca ctgcagaacg cggtctgcat cgcgccggtc gtcgcgctgg
     3061 ccgcgatcat gccgttcacc gtgaccgacc cgtggaaggc ggccggagcg gtggccgcgg
     3121 tggtgctact gaacgcggtg ctgtcgatga cgctctacgt ccgcggggtc aacgagtacg
     3181 gtgccggcgc ggtggcgatg ctgttcgcgg tcatcccggc ggtcgcgggc ctgctgtcgt
     3241 ggctgatgct gggccagcga cccgacatcg ggatcgccat cggcctggtc gtcggcgcgg
     3301 cggcgtgttc cctagctcgc gctgggatcc caaggctcaa tgcccgcgtg tcacgccagc
     3361 agcgtcagca cgatccagcc ggcgacggca gagggcagca tcgcgtcgat ccggtccagg
     3421 attccgccgt gccccggtag caacgtgccc atgtccttga tgccaaggtc gcgcttcacc
     3481 tgagactcga ccaggtcgcc gaggacgccg gtgatcacca gcatcagccc cagcggtacg
     3541 ccgacccagg cgggtttgtc catcaagaac gccaccgaca agacggcggc cgtaatgccg
     3601 aacagcagcg agccgcccag cccctcccac gatttcttcg ggctgatcgc cggcaccatc
     3661 agatgcttac cgaacaacac cccggcgaca tagccgccga tgtcggcgaa aacgacggtc
     3721 gcgatcacgg tgaacgttcg gatgccaccg cggtcttgga agatcagcag cgcactgaag
     3781 ctggcgaaca gcggcaccca cgtcgcgagc agcaccgtgg ccgaaatatc gcgcagatag
     3841 ttcaccggct gctcgctgag accgtgcccg acgaggcgcc acaccatgca caccacgatc
     3901 gttccgccgt acgcccccaa gagtccggcc gggccatagg gccacgtcag ccagatcatc
     3961 gcctggccgc cgatcagcag gggaatcagg ggcagggcgt agccggcctc ccgcagccgg
     4021 cggacgactt cgtgcgtggc aatcggcatc gccaccgcga gcacgggcag ccatccgatc
     4081 ggcgcgaaga gcaggacgcc gatggccatc gcgcccaaga gcacgccgac agcgatcgcg
     4141 gccggcagat cgcggcctgc gcgggacgct tttttcggcg gctcggtcgg gccggcttca
     4201 gtgtctgcca cgggagtcgc tcgctgttcg gtcactagac ctccagcagc tccccttctt
     4261 tgtgcttgac cagctcgtcg atctgggccg tgtaagtgtg ggtggtcttg tcgaggtcct
     4321 tttcggcacg ggatacctcg tcttcgccgg cctccccgtc cttcttgatg cggtgcagct
     4381 cttccatcgc cttgcgacga atgttgcgca ccgagacccg cgcgtcttcg cccttggact
     4441 tggcctgctt gaccagttca cggcgccgct cctcggtcag ctgcgggatg gcgatgcgga
     4501 tgacgttgcc gtcgttggac gggttcacgc cgaggtcgga gttgcggatc gagtcctcga
     4561 tcggcttcag ctgcgactgc tcgtacggct tgatgacgac gagccgggcc tccgggacgt
     4621 tgatgctcga cagctgggtg atcggggtgg gcgagccgta gtagtcaacg ttgatgcggg
     4681 agaacatgcc ggggttggcc cggccggtgc ggatcgacga cagatcgtcg cgcgccaccg
     4741 acaccgcctt ttccatcttc tcctcggcgt cgaagagggt ttcgtcgatc actgcgtact
     4801 cccgtcaggt ggtgaccagt gttccgatct tctcacctgc gaccgcccgc gcgatattgc
     4861 cgtcgacgag caggttgaac accaggatcg gcatgccgtt gtccatgcac aggctgaacg
     4921 cggtggcgtc ggcgaccttc aggccccggt cgatgacctc gcggtggctg atcgcggtga
     4981 gcagctcggc gtcgggctct atccgcgggt cggccgtgaa gacgccgtcg accgccttgg
     5041 ccatcagcac cacctcggca ccgatctcca gcgcgcgctg cgcggcggtg gtgtcagtgg
     5101 agaagtacgg cagacccatg ccggccccga agatcacgac acggcccttt tccaggtgac
     5161 gcacggcgcg cagtgggata tagggctcgg cgacctgtcc catcgtgatc gcggtttgca
     5221 cgcgcgtcgc gatgccttcc ttttccagga agtcttgcag cgcaaggcta ttcatcacgg
     5281 tgccgagcat gcccatatag tcgctgcggg tgcgttccat gccgcgctgc tgcagttgag
     5341 cgccgcggaa gaagttgccg ccgccgatga cgacggcgac ctgcactccg cttcggacca
     5401 cctcggcgat ctgccgggcc acctggtgca cgacatcggg gtccaacccc acttcgccgc
     5461 cgccgaacat ctcgccgccg agcttgagca gcacgcggga gtacatcggc cggatcggtg
     5521 gttcgttgcc gttggtggtg gtcggttccg ccatccgact ccttcggggt cctctcaaga
     5581 gatcgcccct aatcctgccc catagcggcc cgatcgccgc tatggggtgc ccgcgtggcg
     5641 agttagccgg gcaggtgcgc ggtcagcagg aaggccggca ccgaccggcg gcccttcggc
     5701 tcctcccgaa cgtcggtcac cgggaagaac tgctcgaagc cgtccgggaa cacgccgtgg
     5761 atccgggccg ggcggatctc gtcgatggtc cagtacttcg acacgatgtc gcgcagctcg
     5821 tctttagtca ccggattggc agggctgtcg ggcatgcctg ccttgtcgaa caccaacacg
     5881 aagtacgacg cgccgggggc cgccgcgcgc acgatcgatt gctgatagcc ctcgcgcaat
     5941 tcgacaggca tggaatggaa aagagttgag tcgacgatgg tcccaaagcg gccgtcgtag
     6001 ccgtcgaaat tgctgatgtc ggcgacgtcg aaggccgcgg tggtcagtcc ccgcttggcc
     6061 gcctccgccc tggccaagtc gatcgccgtg ggtgactgat ccagcccgac tgtggtgaat
     6121 ccgcgttcag ccagatgcag ggagatggcg gcttcaccgc agcccgcgtc gagcacgtcg
     6181 ccgtggaact tgccctgctc gatcagcgcc gcgagctccg gctgtggttc gccgatgctc
     6241 cacggcggct tcataccgga tcccagctcc tcggcctcgc ccttgtaagc gctttcaaac
     6301 atctgatcca tcggttgagt catgcctccg gtatatcaat ctggttgata tgtgtcaata
     6361 tgcttgatat ggacgagccg tttgaggacc aaccgctggg ctacctgctg agccgggcga
     6421 tgttcgcgct gaagtccgat gtgacggcga cggtgctgga cccgctggaa gtggcgttcc
     6481 ccgaatacct ctgcatgcgg gtgctgtcga aattcccggg gcagtcgaac gccgacctgg
     6541 cccgcgccct gaacgtctcc ccgcaggcga tgaacatggt cctgcgcggg cttgaggatc
     6601 gcggactcgt cactcggccg accagcgtgt cgtcgggccg gtcgctgccc gctcagctga
     6661 cccgcgacgg cgaggaactg ctgaagcgca ccgacgccgg tgtgcaggcc gccgagcgcc
     6721 ggctgatggc caatctcacc gacaaggagc ggtacgactt caagcgcatc ctggccgcgc
     6781 tcggctccga cccgttctag atccccgttg ccgtcagaat gctcacatga ccggtcccat
     6841 ctacgtctcg cgactgctta ccgaccgcgc aatggcgcac cttcgctcat tggatacgcc
     6901 gctgagggtg ggtggcgaag ctccgccaag ccgagccgaa ctcgagagcg ggattgccgg
     6961 cgcggcggcc gcggtcgtca cgttgaccga acgcgtcgac gccgacctgc tcgccgctgc
     7021 agggtcgcaa ctgaaggttg tcgccaatgt ggcggtgggc tacgacaaca tcgacgtgga
     7081 cgccgccgag gccgcgggtg tgatcgtcac caacacgcct ggtgttcttg atcgtgcgac
     7141 agccgatcac accttcgcat tgatcctcgc cgcgagccgt cgaatcaccg agggcgaccg
     7201 gttgatccgg tctcgcgaac cgtggatctg gggaccccgg atgcttgtcg gcctcgacgt
     7261 cagcgccggg gcaaccctcg gcatcttggg gtacggccgc atcggccgcg ctgtggcgcg
     7321 gcgcgcccaa gcctttgaca tggcggtggt ggcgatgagc cgaagtcgcg agcctggcac
     7381 cactgaggac gacgtcacat tcgtcgacac cgcaacgctg ctggccgaca gcgacgtcgt
     7441 caccgtcctt accccgctga caccggaaac gcatcatctg atcgatgccg aggcgttgac
     7501 agcgatgaaa tcgactgcgt acctgattaa tacggcgcgc ggcgggatcg tcgacgaagc
     7561 cgccttgatc gccgcgctgc acgccggcga gatccgcggg gcggcgctcg acgtcttcga
     7621 agacgaaccc aacgtcaacc cggcgctgct cgacgcgccg aatctcgttc tgacgccgca
     7681 tatcgccagt gccggcgagg cgacccgcga cgcgatgggc atcctcgcca tcgacaacgc
     7741 ggcggccgtt ctcgccggca atccgccact gacccctgtg cgttgaggac ttagtggtgg
     7801 ttgcaggccg cgggcggtga gggcgggatg tccagggccg gattgccgtc gaagaagccc
     7861 accggcttga gatggaatcc ggtgtaggcg cacggcatca ccggccagtc ctccggccgc
     7921 accacgtgat gcgcgccgac ggtgtaccag agcaccacgt cggtgtcctc cagcggcgcg
     7981 tcgtccgcga tgtactccgg caggccctgc atatcggacg actggtacat gtagtcgccc
     8041 gccgcgaact tctcggcggg gtcatacttc gtcacccaca agttgtgctg cacgaaccgc
     8101 gcccggtcgt agatgtagga gccttcctgc accatcaccg gtacgacctc cctcggcgtc
     8161 agcttgtacg ccaccgggcc gccgagctcg ttcttcttcg acgggttggt gatcttccaa
     8221 taccggccct tcgaccagtc ccagtcccgc gccccgtcgg cctcggaagc caccaaggtg
     8281 tcgcgggtga tccacgcgtt gtgatgcggg ttcagctccg ggtccggttc ggggatcgag
     8341 tcgacttcgt agacgctgtt gcccgccccg tcgacgctca tgtcgagccg gaagttgaag
     8401 aagtgctggt gattcgggcc atagatgttg ggcgcgacca gctttcccca tcgcggttcc
     8461 tcaccgtcct tgacggcccc ggtggtcagc accccggaca gcttgacctc gacctcgatc
     8521 gacgcgtcgt tgtagaagta ccagaagaat ccgtactcat agttgccgac ggtgcagatc
     8581 atcgagatca ccagccggcg ggcgcgtcgg acttccacct cgccggtacg gaaatcggtg
     8641 tgcttccagg agattccgta gtcttcctcg tgcatgcaga tcgcgttggg gatggtgacg
     8701 gcgttgcctg cagagtcatt gaccgtgccg tcgaagtagt agatttcacc cagacagtcg
     8761 cagcccaggg taagcgggtt ggcggaaaag cccatcccga cttcacccat atcgaagacg
     8821 ttcttgttcc agtgcgttgg ctcggtatca ccgtatggca caaccatttc cgacagcgag
     8881 gctcgataga tgatcggccg agtctcgccg cggtcggtgt aggtgatctc gtgaagggag
     8941 atgccttcgc gtgggttgaa gcccacccgc atcgaccatt tctgccactg caccttccac
     9001 ccgtcgacgg tgaagctcgg gccgtcgggc tgggtgattt cgatcggctt gacgtcgtcg
     9061 cggaactggc tgaacgcagg ccggttgttc tcgtcgaaca tgaactgctc ggcgtagtta
     9121 cccgcggtcg gcggcagcgg cacggcaccg tggtcctcga tgtcgatgac tttcatggcg
     9181 tcgaggtcga aggtgacgat caggccctcg acgggtcgcg cgtacccgtg ctcggagggt
     9241 gcggcgcgca tgaacgtcag cgggcgacag atcagcggcg agttgtcgta gtgatcctgg
     9301 ctgccgtaat agcctgcggg ccaagggtcg atcatggcca ggtcgaaatc gctgacgccg
     9361 cgcttgcgca tggcgtcctg ccagcgcggg tcagcccgca ccaccgcttc cacgccggtc
     9421 atgtgctcga cgaggtagga cggaaaccgc cctggcaccg ccgtccacga gtcgatgacg
     9481 cgcgccccga tgtcgacgat cgcctcgtaa accaacttgg ccgcgccgtc gtacatggat
     9541 acgaacgcgc gtcggggcac gtcaaccacg ccgccgaagg tcagccccgg cgtcttgtcc
     9601 ggctcggcca actggatcat cacgaatttc agtgttggcg tggcgtattc ggagtcggtg
     9661 atcactgtcg cggcggtctc gatctccgcg ccggtcaacg gatccagtgg ataccgcgcc
     9721 gtgttgtcaa tcgcctccgt gctgggcacg gtgctttcca ccgtcatttg gcatcgtcct
     9781 tcaactcgag aaccgccaga tgtgccagtg cgtcttcctg agtgaccttg gccgccgatc
     9841 gcctgccgaa gaagtaaacg atggcgccga gcacgaacat gccgaccgcg atgctgccga
     9901 cccaggtcat ccaggttgcc gccggaacat cgatccaggg cgaagcgaac gagtagtaca
     9961 cgcctgcgca cgtcgcgatg atgccgatcg ccgcgaccgc gtaaaccgcg ggcatgccgc
    10021 cgggaatcct ccagaagtcc tcggactcat agcgatccgc gtacttcttg cgtgcaatca
    10081 ccagcggaac gaagaagaag aatccg
//
LOCUS       DASRJA010000526         9270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4139989,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000526 DASRJA010000000
VERSION     DASRJA010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9270
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4139989"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFC01_23010"
     CDS             complement(<1..654)
                     /locus_tag="VFC01_23010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN82520.1"
                     /translation="MTERAPIHLGSGEPILLLHPFMMSQNVWKNVAPLIAETERYEVF
                     APTMPGHNGGTKGPFFLDSGSLADDVERRLDALGWETAHIVGNSLGGWVAFELERRGR
                     ARTLTGIAPAGGWRHFTPAKFEIVGKFLAGMPIWLFTLAFGERVLKLPFTRYLAHLPV
                     SATPEGLSDDDLRDIIDDVSHCPAYYQLLVKSLTLPGLLELAEGTTPTHLVICEKDRV
                     "
     gene            717..1508
                     /locus_tag="VFC01_23015"
     CDS             717..1508
                     /locus_tag="VFC01_23015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZN82521.1"
                     /translation="MIVSTINVNGIRAAIKQRSAENLGLLPWLAQTRADVVCLQETRA
                     DDDQLALALAPALSDGWHLASAGGDRKGRNGVAVLSRRPIKSVRDLGVDEFALHGRYV
                     EVDIDGLTVASVYIQTGEAGTDRQLEKERFMAALAHRMSTLTDRDAVVCGDWNIAHTE
                     NDIKNWRGNVKKSGFLPAERQWLTGLLDAGWVDVVRVLHPDVAGPYSWWSWRGRAFDN
                     DAGWRIDYHLATRGLAARASAARVERAAAYALRWSDHSPVTVEYG"
     gene            1518..2882
                     /locus_tag="VFC01_23020"
     CDS             1518..2882
                     /locus_tag="VFC01_23020"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN82522.1"
                     /translation="MTTASGAPRRHRVVIIGSGFGGLFAAKHLKRADVDITLIAKTTH
                     HLFQPLLYQVATGILSVGEIAPATRMILRKQKNAQVLLGDVVGIDVKNKTVTSKLLDW
                     ERVTPFDSLIVAAGAQQSYFGNDHFEAFAPGMKTVDDALELRGRILGAFEAAEVTTSE
                     AERQRRLTFVVVGAGPTGVEVVGQISELAHRTLTGAFRTIQPGEARVILVEAAPAVLP
                     PMGPKLGLKAQRRLEKMGVEVRLNTMVTDVDYMGLTVKEKDGTEHRIDCAVKVWSAGV
                     QASPLGKLIAEQSDGTETDRAGRVVVEPDLTVKGHPNVFVVGDLMSVPGVPGQAQGAI
                     QGAVYASKQIKAGLKGEDPAARKPFKYFDKGSMATVSRFSAVCQIGKLEFGGFIAWLA
                     WLGLHLYYLVGGRNRLVAVISWFITFLGRGRGQMTITERWVFARRAIEEVRDQQKETP
                     RAIG"
     gene            2971..3987
                     /gene="trpS"
                     /locus_tag="VFC01_23025"
     CDS             2971..3987
                     /gene="trpS"
                     /locus_tag="VFC01_23025"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZN82523.1"
                     /translation="MSTSAARVVFSGVQPTSDSLHLGNALGAVKQWVGLQDEYDAYFC
                     VVDLHAITLPQDPEQLRRRTLVTAAQYLALGIDPSRSTIFVQSHVPAHAELAWVLGCF
                     TGFGQASRMTQFKDKSQKQGTDSTTVGLFTYPVLQAADVLLYDTDLVPVGEDQRQHLE
                     LARDVAQRFNSRFPDTFVVPDVLIQKATAKIYDLQDPTSKMSKSAATDAGLISLLDDP
                     KASAKKIRSAVTDSEREIRFDQDAKAGVSNLLTIQSAVTGADIDKLVEGYAGRGYGDL
                     KADTAEAVVEFVTPIKARVDELLADPAELEAVLATGAERARDVSAKTLKRVYDRLGFL
                     APRA"
     gene            4006..5028
                     /gene="yhjD"
                     /locus_tag="VFC01_23030"
     CDS             4006..5028
                     /gene="yhjD"
                     /locus_tag="VFC01_23030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HZN82524.1"
                     /translation="MTEPAKPGILDRLRARYGWFDHAMRAQERYKDSKGDFYAAGITY
                     FTVFALFPLLMVGFAIAGFVLASQPDLLAEIEDKIKSAVSGDFAEQLVKLMESAIDSR
                     TSVGIIGLATAAWAGLGWMANLREALTQMWGLMRHEPPGFLRTKLSDLTAIVGFFVAI
                     VVTIALTALGSSGVMKRILELVGLQDVPGVSLALRVASLLVSILVSWLLFIWVIARLP
                     REKVTFRSAMRAALLAAVAFEIFKQVGSIYLRAVVTGPAGATFGPVLGLMVFAYITAR
                     LILFATAWAATSAENLAEEPVEPPAPAQITVRVREHEGLGVRGALTAAVVGALGALGF
                     SRLRRH"
     gene            complement(5025..5279)
                     /locus_tag="VFC01_23035"
     CDS             complement(5025..5279)
                     /locus_tag="VFC01_23035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82525.1"
                     /translation="MAATAKAYAICGLRFGVVRRIPACQGRGAEAGNAEELPSIASVQ
                     PLAAIEGVRRQWSEAAKACVIDGSHKNTFARPIRLASTGS"
     gene            5269..6702
                     /locus_tag="VFC01_23040"
     CDS             5269..6702
                     /locus_tag="VFC01_23040"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN82526.1"
                     /translation="MAANDLGWLDAVALRDLVADAEVTPAEVVEGAIARIEAANPELN
                     AVVVPLPDIGRADAADPALPRGPFYGVPFLLKDAGACLAGLPLYMGNGLLRSLDWRAP
                     SDTILGDRFRAAGLVTVGKTALPEFGCQPTTQPIAFGACRNPWDIQRSTAGSSGGAAA
                     AVAAGLVPAAHASDIGGSIRLPAAWCGVVGLKPSRGRTSSLPITDPNLVEHVITRSVR
                     DTAAFLDAVGGADPADTYRLPPPTVPYATTMSDAPSPLRIGYVESVEAIGVTVARDCV
                     AAVTEAAGLLEALGHHVEPRQPQRLFDEEFATHHLRSVGWEFQHMLDGLAAAIGRPLT
                     AADVEPYSWALASVGASLTDASYSASQTWQRAYTAEVSAWWAGDFDLLLTPAAGEPPA
                     LLDELLPPPADPLSILPRFERIWCFAAPFSVTGQPAISLPIGLTASGLPVGVQLVAAL
                     GREDLLLRVAAQLEAAAPWRHRRPPVL"
     gene            complement(6911..7033)
                     /locus_tag="VFC01_23045"
                     /pseudo
     CDS             complement(6911..7033)
                     /locus_tag="VFC01_23045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138585.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
     gene            complement(7113..7547)
                     /locus_tag="VFC01_23050"
                     /pseudo
     CDS             complement(7113..7547)
                     /locus_tag="VFC01_23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931684.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
     gene            7843..8892
                     /locus_tag="VFC01_23055"
     CDS             7843..8892
                     /locus_tag="VFC01_23055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003938333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZN82527.1"
                     /translation="MPASHIYVQHLSRPSGADPVVRLDDPVPADGQTVPGGWWPPLML
                     EPGWVSDNHRRFDVFHVHFGFDAIGPDVLTAVVQELKEHDKPLVYTVHDLRNPHHDQP
                     DAHAEQQDVLLAAAHTVITLTPGAARAISRRWNRSAHVLPHPHLLSSAAIGRNRDHGE
                     RFVVGVHAKSLRANMDPLPVIDALVVALAELPGAVLRVDVHDEIFDSDNYWFAPEVGN
                     AILSYGRSDHVSVRVHPYFSDDQLWEYLASLTVSVLPYRFGTHSGWLEACFDLGTAVI
                     VPSCGFYHQQRPCGVFEFAEDRFDVSSLRRAVHAAYRRWRAGVAAPRATWAERRTERN
                     GIAEAHHRLYEGAVS"
     gene            8889..>9270
                     /locus_tag="VFC01_23060"
     CDS             8889..>9270
                     /locus_tag="VFC01_23060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN82528.1"
                     /translation="MSAGLRIALIGSSRFPIRQPFPGGLEAHVWHLARALADRGHEVS
                     LFAAPGSDPALGCETLAVRPLELSEAARSDACMPAVTFMAEHHAYLSLMLQLGRSGAK
                     HFDLVHNHSLHHLPVAMAPMLPIPM"
BASE COUNT         1495 a         3033 c         3121 g         1621 t
ORIGIN      
        1 cacgcggtcc ttctcgcaga tgacgaggtg cgtcggcgtg gtgccctcgg cgagctcaag
       61 cagacccggc agcgtcaacg acttcaccag cagctggtag taagccgggc agtgggagac
      121 gtcgtcgatg atgtcgcgca ggtcgtcgtc ggacaggcct tcgggggtgg cgctgaccgg
      181 cagatgcgcg aggtaccggg tgaacggcag cttcaagacg cgctcaccga acgccagcgt
      241 gaacagccag atcggcatgc cggcgaggaa cttgccgacg atctcgaact tcgcgggggt
      301 gaagtggcgc cagccgccgg ccggtgcgat tccggtcagc gtgcgggccc ggccgcggcg
      361 ctccagctcg aacgcgaccc agccgccgag cgagttgccg acgatgtgcg cggtttccca
      421 gcccagcgcg tcgagccggc gttcgacgtc gtcggccagc gacccggagt ccaggaagaa
      481 cgggcctttg gtgccgccat tgtggccggg catggtcggc gcaaagacct cgtaccgctc
      541 cgtctcagca atcagcgggg caacgttttt ccacacgttc tgcgacatca tgaagggatg
      601 tagcaaaagg atcggctcgc cggaacccag gtgtatcggt gcgcgctcgg tcatgatgcc
      661 cgacattaaa ggtgataccg ccggtaccgc aagcctgtcg gctacggtct gaccccgtga
      721 ttgtcagcac aatcaacgtc aacggcatcc gcgccgcgat caagcagcgg tcggcggaga
      781 acctcggcct gctgccgtgg ctcgcccaga cgcgggccga tgtggtgtgt ctgcaggaga
      841 cgcgggccga cgacgatcag ctcgctttgg cgctggcgcc cgccttgtcc gacggttggc
      901 atttggcatc cgcgggggga gatcgcaagg gccgcaacgg cgttgcggtg ctgagccggc
      961 gtccgatcaa gtcggtgcgg gatctgggcg tcgacgaatt cgcgcttcac ggccgctacg
     1021 tggaggtcga catcgacggg ctgaccgtcg cgagcgtcta catccagacc ggggaggccg
     1081 gcaccgaccg ccagttggag aaggaacggt tcatggcggc gctcgcgcac cggatgtcga
     1141 cgctgaccga tcgcgacgcc gtcgtatgcg gcgactggaa catcgcgcac accgaaaacg
     1201 acatcaagaa ctggcgcggc aacgtcaaga agtcggggtt cctgccagcg gaacggcagt
     1261 ggctcaccgg cctgctcgac gcgggttggg tcgacgtggt tcgggtgttg caccccgacg
     1321 tggcagggcc gtattcgtgg tggtcatggc gtgggcgggc gttcgacaat gacgccggct
     1381 ggcggatcga ctaccacctg gcgacgcgtg gcctggcggc ccgggcgagc gccgcgcggg
     1441 tggaacgggc cgccgcctac gcgctgcgct ggtccgacca ctcgccggta accgtcgaat
     1501 acggttaggg tcggtccgtg accaccgcaa gcggtgcccc tcggcgccac cgcgtcgtca
     1561 tcatcggttc cggcttcggc ggactgttcg ccgccaagca cctgaagcgc gccgacgtcg
     1621 acatcaccct catcgccaag acgacgcacc atctgttcca gccgctgctc tatcaggtcg
     1681 cgacgggcat cctctccgtc ggcgagatcg ctccggcaac gcgaatgatc ttgcgcaaac
     1741 agaagaatgc gcaggtgctg ctcggcgacg tcgtcgggat cgatgtgaag aacaagaccg
     1801 tcacgtcgaa gctgctcgac tgggagcggg tgaccccgtt cgacagtctc atcgtggccg
     1861 caggcgcgca gcagtcctac ttcggcaacg atcatttcga ggcctttgcg cccggcatga
     1921 agaccgttga cgacgccctc gaattgcgcg gccggatcct cggagcgttc gaggcggctg
     1981 aggtaacgac gtcggaggcc gaacggcaac gccgactgac ctttgtggtg gtcggcgcgg
     2041 gcccgacggg cgtcgaagtc gtcgggcaga tctccgagct cgcgcaccgc acgctgaccg
     2101 gcgcattccg gacgatccag ccgggcgagg cccgggtgat cctggtcgag gccgcgcccg
     2161 ccgttttgcc gccgatggga ccgaagctcg gactcaaggc acagcgtcgg ctcgagaaga
     2221 tgggtgtcga ggtccggctc aacacaatgg tgaccgacgt cgactacatg gggctgacgg
     2281 tcaaggagaa ggacggcacc gaacatcgca tcgactgcgc cgtcaaggtc tggtcggccg
     2341 gtgtgcaggc cagcccactc ggcaagctga tcgccgagca gtccgacggg accgaaactg
     2401 atcgggccgg ccgggtggtc gtcgagccgg accttacggt gaagggccat cccaacgtct
     2461 tcgtcgtcgg ggacctgatg agcgtgcccg gtgtccctgg ccaggcgcag ggtgcgattc
     2521 agggcgcggt ctacgcctcc aagcagatca aggctggcct caagggcgaa gatcccgcgg
     2581 cgcgaaagcc gttcaagtac ttcgacaagg gcagcatggc gacggtgtcg cggttcagcg
     2641 cggtgtgtca gatcggcaag ctcgagttcg gcggcttcat tgcctggctg gcgtggctgg
     2701 gcttgcacct gtactacctt gtcggcggcc gcaaccggct ggtcgccgtc atctcgtggt
     2761 tcatcacctt cctcgggcgc gggcgcggcc agatgacgat caccgaacgg tgggtattcg
     2821 cacgtcgggc catcgaggaa gtccgagatc agcagaagga aactccgcgg gccatcgggt
     2881 gatttcggcg cgctaaccgt cgctgagcgt cgctgagcgt ggataaccgc gccgaaatcg
     2941 ctcgcatccg tgtgaaaaga ttgacccatc atgagcactt ccgctgcgcg cgtcgtcttc
     3001 tccggcgtgc aacccacctc tgactccctg cacctcggca acgcgttggg ggcggtcaag
     3061 cagtgggtgg gtctgcagga tgagtacgac gcctacttct gcgtcgtcga cctgcacgcg
     3121 atcacgctcc cgcaggatcc cgagcagctg cgccgtcgca ctctcgtcac ggccgcgcaa
     3181 tacctggcac tgggcatcga cccgtcgcgc agcacgatct tcgtccagag ccacgttccg
     3241 gcgcacgccg aattggcttg ggtgttgggc tgtttcaccg gtttcggcca ggcgtcgcgg
     3301 atgacgcagt tcaaggacaa gtcgcagaag cagggcaccg actccaccac tgtcggcctg
     3361 ttcacctacc cggtcttgca ggctgccgac gtgctgctct acgacaccga cctggtgccg
     3421 gtgggggagg accaacgtca gcacctcgag ctggcccgcg acgtcgccca gcggttcaac
     3481 tcgcggttcc ctgacacgtt cgtcgtgccc gacgtgctga tccagaaggc gacggccaag
     3541 atctacgacc tgcaggaccc gacgtcgaag atgagcaagt cggcggccac cgacgccgga
     3601 ctgataagcc tgctcgacga tccgaaggcg tcggcgaaga agatccgctc ggcggtcacc
     3661 gacagcgaac gcgagatcag gttcgaccag gacgccaaag ccggggtgtc gaacctgctg
     3721 accatccagt ccgcggtcac gggcgccgac atcgacaagc tcgtggaggg ctacgccggt
     3781 cgcggctatg gcgatctgaa ggccgacacc gcggaggccg ttgtcgagtt cgtcacgccg
     3841 atcaaggcgc gcgtcgacga actgctcgcc gatcccgccg aactcgaagc cgtgctcgcg
     3901 accggcgctg aacgcgcaag agacgtgtct gcgaagacgc tcaagcgggt atatgaccgg
     3961 ttagggttct tagcgccacg cgcgtaacac tctcgggagg ccgggatgac ggagccggcc
     4021 aaaccgggga ttctggatcg attgcgggcg cggtacggct ggttcgacca cgcgatgcgg
     4081 gcgcaggagc gctacaagga cagcaagggc gacttctacg cggccggtat tacctacttc
     4141 acggtgttcg cgttgttccc gttgctgatg gtgggattcg cgatcgctgg cttcgtcctc
     4201 gcgagccagc cggatctgct cgccgagatc gaggacaaga tcaagtcggc ggtgtccggc
     4261 gacttcgccg aacagctggt caagttgatg gagtcggcga tcgactcgcg cacctcggtc
     4321 ggcatcatcg gcctggccac cgccgcgtgg gcggggctgg gctggatggc gaacctgcgt
     4381 gaggcgctga cccagatgtg gggtctcatg cgccatgagc cgcccggatt tcttcgcacc
     4441 aagctgtccg atctgactgc catcgtgggc tttttcgtgg cgatagtcgt gaccatcgcg
     4501 ctgacggcgc tgggcagttc gggagtgatg aaacggatcc tggaattggt tgggctacaa
     4561 gatgttccgg gtgtcagtct cgcgctgcgg gtcgcgtctc tcttggtgtc gattttggtc
     4621 tcgtggctgc tgttcatctg ggtcatcgcg cgattgcctc gggagaaggt gacattccgc
     4681 agcgcgatga gggccgcact gctggcggcg gtggccttcg agatcttcaa gcaggtgggc
     4741 tcgatctatc tgcgagctgt ggtgaccggt ccggccggtg cgacgttcgg tccggtgctg
     4801 ggcctgatgg tgttcgccta catcaccgca cggctgatcc tgttcgcgac ggcctgggcc
     4861 gcaacatcgg ccgaaaacct ggcggaggaa ccggtcgagc ctcccgcacc tgcccagatc
     4921 acggtgcgtg ttcgggagca cgaagggctc ggtgtgcgcg gggcattgac cgcagcagtg
     4981 gtcggagccc ttggtgcact gggattttcg cggctccgac gacactagct gccagtgctg
     5041 gcgagcctga taggtcgggc gaaggtgttc ttgtggctgc cgtcgatgac gcacgccttt
     5101 gcggcctctg accactgccg ccggacgccc tcgatcgctg ccagcggctg caccgaggcg
     5161 atactcggca gttcctcagc gttcccagcc tcggcgccac gcccttggca cgccggaatt
     5221 cggcgcacaa caccgaaacg cagtccgcag atcgcatagg ctttcgcggt ggccgccaac
     5281 gatctgggct ggctcgatgc ggttgccttg cgcgatctgg ttgccgacgc cgaggtcacg
     5341 cccgccgagg tggtagaggg cgcgattgcg cgcatcgagg ccgcaaaccc cgagctgaac
     5401 gccgtcgtgg tgccgctgcc ggacatcggg cgcgccgacg ccgccgatcc cgcgttgccg
     5461 cgggggccgt tttatggagt gccgtttcta ctcaaggacg ccggcgcatg cttggccggc
     5521 ctgccgctgt acatgggcaa cgggctgctt cggtcgctgg actggcgggc gccgtccgac
     5581 accattctgg gcgaccgctt ccgcgccgcc ggcctcgtca ctgtcggcaa gacggcgctg
     5641 cccgagttcg gctgccaacc gaccacccag ccgattgcct tcggcgcgtg ccgaaacccg
     5701 tgggacatcc agcgctccac cgcgggatca agcggcggtg cggccgccgc cgtcgcggcg
     5761 gggctggtgc cggcggcgca cgccagcgac atcggcggct cgatccggct gcccgcggcg
     5821 tggtgcgggg tcgtggggct caagccgtcc cgcggacgca cgtcgagcct gccgatcact
     5881 gaccccaatc tcgtcgagca cgtgattacc cgcagcgtgc gcgacaccgc tgcgttcctc
     5941 gacgccgtgg gcggtgccga tccggccgac acctaccggc tgccgccgcc gactgtgccg
     6001 tacgcgacga cgatgtcaga tgcaccgagc cccctgcgca tcggctacgt cgagtcggtc
     6061 gaggcgatcg gggtgacggt cgcccgcgac tgtgtcgccg cggtcaccga ggcggctggg
     6121 ctgcttgaag cgctcggcca ccatgtcgaa ccgcgccaac cgcagcgcct gtttgacgag
     6181 gagttcgcaa cgcaccacct gcgatcggtt ggctgggagt ttcagcacat gctcgatggg
     6241 ctcgccgccg cgatcggacg gccgctgaca gcggccgatg tcgagcccta ttcgtgggcg
     6301 ctcgcgagcg tgggcgccag cctgaccgac gcttcttact ctgccagcca aacctggcag
     6361 cgcgcgtaca cggccgaggt gtcggcttgg tgggcaggcg acttcgacct gcttctgacg
     6421 cccgctgccg gcgagccgcc cgcccttctc gatgagctcc tgccgccacc cgctgacccg
     6481 ctgtcgatcc tgccgcggtt cgaacgcatc tggtgcttcg cggcgccgtt cagcgtcacc
     6541 ggccagccgg ccatcagcct gccgattggg ctgacggcaa gcggactgcc ggtcggcgtc
     6601 cagctcgtcg cggcgctcgg gcgcgaggat ctgctgctgc gcgtggcggc acagctcgag
     6661 gcggctgcgc cgtggaggca tcgccgtccg ccggtgctct gagacaattt cacgcgcagt
     6721 cgcgcccgag aaacaggcgt gtccaaggcg ttctgtggtt cctaccagct agatgcccgg
     6781 gtggatgttt tgatcaatcg ggtggatgga ggccgaaact gtgcaccaca tcattttcgg
     6841 tgagtgttcc atcgaagacc ggctcaccac gcgcgtgatg gattgttggc aagcgggaca
     6901 ctcgtcgcta cgcgaccatg gaccgatcgg actccaccac gctgccgtcg gccatctcga
     6961 tgcgcaccgg cccgtgcacc agcgccggcc cggctggcat cagcggggac ggtgtcggtt
     7021 cgttctcggt catggcttgt ccgcccggat gaccaaaagc tcttcttcgc ggcggccggg
     7081 ttcgagccgg ccagtgtctt cgagccgtcg acggccgttt ccttgaggaa caggtcttgg
     7141 ccgtcgccgc ggagctgcac cagcgcgatg tcggtcaaac ccggcctcca gtaggcccgt
     7201 cacggcctcg acgatcgcgt cgagatccgg cccgcgcgga atggaccgac ccgccacgtc
     7261 ctctgggcgc acgaactgcg gagcgtcctc gaaagcccgc cgtcgtcggc aaatcggcgt
     7321 tgaccgcccg gccaccgccg atcagcggaa ctgatcatgc gcgcccccgc actgctacgt
     7381 cgcagtctgg gcccggcaga tcggatctgc cggatcatcc tgacgtcgcg gggcagggcg
     7441 gtcgccggtc gcgcggcgta ggacgcgtcg acgagctgct tgttcggctc gaccgcgatc
     7501 agatggtcgg acaggggcgc gaacaagtcg gccgagcgct cgccggattc acaggcggtg
     7561 gcgccggcgt cggccctcag gttcaagatc cttcggccga agcgcgtact cgagtgcgcg
     7621 cttgatccca gcacgccgaa gccgctcgtg ggagtccact atgaacatct ttcccgtttc
     7681 aacacgccac cgtcgggtat tacgtccgca gcatccctcc agcgccgggg gaactgccgc
     7741 tcagacaggc agacgcaaac gtatacacgc gcataggttg tccgacccga tcgagtgagc
     7801 cgtaagaccg attgtgggag gttgtcgttg cgcatcgcct cagtgccagc ctcgcatatc
     7861 tacgttcaac acctgtcgcg acctagcggc gcggatcccg ttgtgcggct ggatgatccg
     7921 gtgcctgcgg acggtcaaac cgtgccgggc ggctggtggc cgccgctgat gctcgaaccc
     7981 gggtgggtca gcgacaacca ccgccggttc gacgtgtttc acgttcattt cggtttcgac
     8041 gccatcggtc ccgacgtgct gacggcggtc gtacaggagc tgaaagagca cgataagccg
     8101 ctcgtgtaca cggtgcatga cctgcgcaac ccacatcacg accaacccga tgcacacgca
     8161 gagcagcaag atgtgctgct ggctgcagca cacaccgtga tcaccttgac accgggagcc
     8221 gcacgggcaa tcagtcggcg gtggaaccga tctgcccacg tgcttcctca cccgcatcta
     8281 ctgagttccg ccgccatcgg acggaaccgc gatcacggtg agcgtttcgt ggtcggtgtg
     8341 cacgccaaga gtctgcgtgc caacatggac ccgcttccgg tcatcgacgc gctggtcgtc
     8401 gcacttgcag agctgcccgg tgcagtacta cgtgtcgacg tccacgacga gattttcgat
     8461 tccgataact actggttcgc gcccgaagtg gggaacgcga tactgagcta cggtcgctcg
     8521 gatcatgtat cggtccgggt gcacccgtac ttttccgacg accaattgtg ggagtacctg
     8581 gcttcactga ccgtctcggt gcttccctac cggttcggca cgcattccgg gtggctggag
     8641 gcctgcttcg acttagggac tgccgtcatc gtgcccagct gcgggtttta ccaccagcaa
     8701 cgtccttgcg gcgtattcga attcgccgaa gaccggttcg acgtctcgtc gctgcgccgt
     8761 gctgtgcacg ctgcctatcg gcgctggcgg gccggtgtcg cggcgccgcg cgccacctgg
     8821 gccgagcgtc gaaccgagcg caacggaatc gccgaggccc accaccgact ctacgaaggt
     8881 gcggtttcgt gagcgccggt ttgcggatcg ccttaattgg atccagccgc ttccccatcc
     8941 gccaaccgtt ccccggcggg ctggaagccc acgtgtggca tctcgcccgc gcgctggccg
     9001 accgcggaca cgaggtgtcg ttgttcgccg cgccgggatc ggacccggcg ctcggctgcg
     9061 agacgctggc ggtgcgtccc ctcgagctct ctgaggcagc acgctccgat gcgtgtatgc
     9121 cggcggtgac gttcatggcc gagcatcacg cgtacctgtc gctgatgctg caattagggc
     9181 gttctggtgc gaagcatttc gacctcgtcc acaaccacag cctgcaccac ttacccgtgg
     9241 ccatggcacc gatgctgccg atcccgatgg
//
LOCUS       DASRJA010000527         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2959235,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000527 DASRJA010000000
VERSION     DASRJA010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2959235"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..908
                     /locus_tag="VFC01_23065"
     CDS             108..908
                     /locus_tag="VFC01_23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZN82529.1"
                     /translation="MSDPQKGPDAIIEQRGHTLILTLNRPEARNALSTEMLSIMVEAW
                     DRVDNDPEIRSCILTGAGGYFCAGMDLKAATAKPPGDSFKDGSYDPSRIDALLKGRRL
                     TKPLLAAVEGPAIAGGTEILQGTDIRIAGESAKFGISEAKWSLYPMGGSAVRLARQIP
                     YTLACDLLLTGRHITAAEAKEIGLIGYVVPDGSALEKALEIAEVINNNGPLAVQAILR
                     TIHETEGLHENDAFKIDTKIGIEVFVSDDAKEGPLAFKEKRAPNFKMR"
     gene            complement(895..>2594)
                     /locus_tag="VFC01_23070"
     CDS             complement(895..>2594)
                     /locus_tag="VFC01_23070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HZN82530.1"
                     /translation="VVLPPGVTLISLIERNIANVGDSVAYRYLDFTRSDAGQLSEITW
                     TQLGVRLEAIGARVQQVTSRGDRVAILAPQGLDYIAGFFAAVKAGTIAVPLFAPELPG
                     HVERLETALNDAKPTVVLTTTAVKDVVDGFLAAMPAHSPHVIAIDEIPDSAAEAFTPV
                     TIDVADVSHLQYTGGATRPPVGVEVTHLAVGTNLLQMILSIDLLDRNTHGVSWLPLYH
                     DMGLSMIGFPAVYGGHSTLMAPTAFIRRPQRWIHALSAGSREGRVVTAAPNFAYEWAA
                     QRGLPDPGSDFDLSNVVLIIGSEPVSIDAIRTFDKAFAPFGLPRSAFKPSYGIAEATL
                     FVSTIEPSAQPTVSYFDRNQLAVGRAVPTAADNPNAVAHVSCGQVARSQWAVIVDPDT
                     GDELPDASVGEIWLHGNNICRGYWGRPDESRRTFHAKLRTRPGDWLRTGDLGVHLDGE
                     LYVTGRIADLIVIDGATHYPQDIEVTAAEASPMVRRGYAAAFTDDSQHLVIVAERAAG
                     TARHDPQPAIEAIRKAVSQKHGLTVSDVRFVPAGAIARTTSGKLARWACRASYLSAS"
BASE COUNT          465 a          824 c          877 g          428 t
ORIGIN      
        1 atggcgtgct cggcgagatc ggcaatgttc agggccacga gacctaaact agaacgtgtt
       61 acattttcat tcaagtctgt ggcatcgacg ctggaaggcg gacacccgtg agcgaccctc
      121 aaaaaggccc cgacgccatc attgagcagc gcggacacac tttgatcttg acactgaacc
      181 ggccggaggc gcgcaacgcg ctttccaccg agatgctctc gatcatggtc gaggcctggg
      241 accgcgtcga caacgatccg gagatccgca gctgcatcct gaccggcgcg ggcggctact
      301 tctgcgcggg catggacctc aaggcggcga cagccaagcc gccgggcgac tccttcaagg
      361 acggcagcta cgacccgtca cgcatcgacg ccctgctcaa gggcaggcgg ctgaccaagc
      421 cgctgctcgc ggcggtcgag ggcccggcga tcgccggcgg cacagagatc ctgcagggca
      481 ccgacatccg catcgcgggc gagagcgcca agttcggcat ctccgaggcg aagtggagcc
      541 tgtacccgat gggcggctcc gcggtcaggt tggcccgtca gatcccctac accctggcct
      601 gcgatctgct gctgaccgga cggcacatca cggccgccga ggccaaggag atcggcctga
      661 tcggctacgt cgtaccggac ggctcagcgc tggagaaggc actggagatc gccgaggtga
      721 tcaacaacaa cggaccgctt gcggtgcagg cgatcctgcg gaccatccac gagaccgagg
      781 gcctgcacga gaacgacgcg ttcaagatcg acaccaagat cggcatcgag gtgttcgtct
      841 ccgacgacgc caaggaaggc ccgttggcgt tcaaggagaa gcgagcgccc aacttcaaga
      901 tgcgctgagg tagctcgcgc ggcatgccca tcgggccagc ttgccgctgg tggtgcgcgc
      961 gatggcgccc gcaggcacga aacggacgtc ggacaccgtc agtccatgtt tctgcgagac
     1021 cgccttgcgg attgcctcga ttgccggttg cggatcgtga cgggcggtac ccgcggcgcg
     1081 ttcggcgacg atcaccagat gctgactgtc gtcggtgaag gccgccgcat agccccgtcg
     1141 caccatcggt gacgcctccg ctgcggtgac ctcgatgtcc tgcggatagt gggtcgcacc
     1201 gtcgatgacg atgaggtccg cgattcggcc ggtgacgtag agctcgccgt ccagatgcac
     1261 cccgaggtcc ccggtgcgca gccaatcgcc cggtcgggtc cgcagtttgg cgtggaaggt
     1321 tcggcggctt tcgtcgggcc ggccccagta accgcgacag atgttgttgc cgtgtagcca
     1381 gatctcgccg acgctggcgt cgggcaattc gtcaccggtg tcggggtcga cgatgacggc
     1441 ccactggctt cgggcaacct ggccgcacga cacgtgcgcc accgcgttgg ggttgtcggc
     1501 cgccgtgggc accgctcgac cgacggccaa ctggttgcgg tcgaagtagc tgacggtcgg
     1561 ctgtgcgctc ggctcgatgg tcgagacgaa cagtgtcgct tcggcgatac cgtaggacgg
     1621 cttgaacgcc gatcgtggta aaccgaaggg cgcgaacgcc ttatcgaagg tccggatcgc
     1681 gtcgatgctc acgggctcgg acccgatgat cagcacgaca ttgctcaggt cgaagtcgct
     1741 gcccgggtcg ggtaggccgc gctgtgcggc ccactcgtat gcgaagttcg gcgcggcggt
     1801 gacgacgcgg ccctcgcgtg agccggccga caaagcatga atccaccgct gcggtctacg
     1861 aatgaacgcg gtgggtgcca tcagtgtcga gtggccgccg tacaccgccg gaaagccgat
     1921 catcgatagg cccatgtcgt ggtacagcgg taaccagctg acgccgtggg tgtttcgatc
     1981 caacagatcg atcgagagga tcatctgcaa gaggttggta ccgaccgcta ggtgggtgac
     2041 ctccacgccg acgggcggcc gcgtcgcgcc cccggtgtac tgcaggtgag agacgtctgc
     2101 gacgtcgatc gtcactgggg tgaaagcctc cgccgctgag tcgggtatct cgtcgatggc
     2161 gatgacgtgc gggctgtgcg caggcatcgc agccaggaac ccgtccacca catctttgac
     2221 ggccgtggtg gtcaggacga cagtcggctt ggcgtcgttt agggccgttt ccagacgctc
     2281 gacatggccg ggcaattccg gtgcaaacaa cggcaccgca atggttcccg ctttgaccgc
     2341 cgcgaagaac ccggcgatgt agtccaggcc ctgcggcgcc aggatcgcca cccgatcacc
     2401 ccgcgaggtg acttgctgaa cacgcgcacc gatggcttcc agccgaacgc cgagctgcgt
     2461 ccaggtgatt tcgctgagct ggcccgcgtc cgagcgggtg aagtccaggt accggtaggc
     2521 gacggagtcg ccgacgttcg cgatgttgcg ctcgatgagc gatatcagcg tcaccccggg
     2581 cggcagtacg acgc
//
LOCUS       DASRJA010000528         9490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3949017,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000528 DASRJA010000000
VERSION     DASRJA010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9490
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3949017"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /gene="ychF"
                     /locus_tag="VFC01_23075"
     CDS             <1..858
                     /gene="ychF"
                     /locus_tag="VFC01_23075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZN82531.1"
                     /translation="DIAGIVKGASEGAGLGNKFLANIREADAICQVVRVFTDDDVAHV
                     DGRIDPKSDIEVIETELILADLQTLERAVPRLEKEARTHKDRRAAHEAAVAAQEILNG
                     GTTLFAAQVDASLLRELNLLTTKPFLYVFNADEAVLTDDARVAALRELVAPADAVFLD
                     AKIEAELQELDDESAAELLESIGQTERGLDALARAGFHTLRLQTFLTAGPKESRAWTI
                     HQGDTAPKAAGVIHTDFEKGFIKAEIVSYDDLIGAGSMAAAKAAGKVRMEGKDYVMAD
                     GDVVEFRHG"
     gene            complement(1090..2196)
                     /locus_tag="VFC01_23080"
     CDS             complement(1090..2196)
                     /locus_tag="VFC01_23080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82532.1"
                     /translation="MHVMLRRYITTGVAIAGASVLVATSVHLPAHRLIPSQPAIAFKL
                     QFLTADLSDGTGPSLLWQNYPTHAAGGAESDIARPPQTASLDPSRNPGILSARISGQV
                     NVPGPLISASLNLPTLNSLDGDIPVVGAAIIRTIALVTQFIFINVPINLANVIAAAVA
                     NPKDIPGLLSFMVHCLLLPEPYAEFGNEYTLLYRLTAPIIGALATVLPPPIGSGANPK
                     TEPGLILTSWKTISDNIKSGLSALPEAVFPAPTFGNVPPPIVPITPEAVIAAPTVDSV
                     PSLIAPVNSKADLRASQTQRKDSKTDTKGKGSDDTPSPTENPRTRAANTDATNTDTSS
                     ENHKHPKGDRRDESRNANDGNSNSGKTPARNASR"
     gene            complement(2465..3034)
                     /locus_tag="VFC01_23085"
     CDS             complement(2465..3034)
                     /locus_tag="VFC01_23085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82533.1"
                     /translation="MLSGKAAVVGVVAASVATAGVVVATLVMDEPGSTPSARAPLPTA
                     AQPYPTAAQPKPAAAGTTQPVKPAVDRTTLPAKPPVAPPIAMDDSGFVNSNARCEGNQ
                     SAVAIGRTNRSVVVVCKGPNNRYQYNGMRLSDRAALQLDDVRVTAQGFEARGDGAKYL
                     ISATELVAMSGDAVLTDPMLEYRTPKPNR"
     gene            3345..4643
                     /locus_tag="VFC01_23090"
     CDS             3345..4643
                     /locus_tag="VFC01_23090"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN82534.1"
                     /translation="MYPRRIAGLVACVVALIAGCSSTTESSTASSATPTATSAAPSKN
                     SALKTIDPKAFQAVVDTAAKQLMVPGAMVLLRTPQGNFSATYGTTELGAQTPPAPNTH
                     FRIASNTKTMTAALIVLLAQDGKLKFSDPVSNYVPGVPNGENITVAELLKMRSGLYGY
                     SADPALAAAMDANPGKAWTPQEVLDIAFRHPPQFAPDASYEYSNTNYALLGLIAEKVG
                     GQPLRQQLAERLFGPVGLAQTSLPATDDTTIPAPYSHGYMYGGSYYALADDPYPAQMQ
                     AAARAGTLKPIDYTNQNSSYATAAGGAISTADDLATWMKALVSGKVFNADYHQQWLTS
                     LQAEDPDAPDGQKYGYGISYQRFGPNAAMFYHGGELPGFNSFMGYDPDNDVALVIWTN
                     LTLSPDGKTTAQAMLATILDEIYAGLSLASPPAPTTTTTR"
     gene            complement(4683..4925)
                     /locus_tag="VFC01_23095"
     CDS             complement(4683..4925)
                     /locus_tag="VFC01_23095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82535.1"
                     /translation="MKTTVKYATPWLAAAAIGAAIAVAPVASAATHPAPVTQAVTPSP
                     NADTGIDPATPYGTDPESPAIFGYHKWLGDDVDVPF"
     gene            complement(5138..5386)
                     /locus_tag="VFC01_23100"
     CDS             complement(5138..5386)
                     /locus_tag="VFC01_23100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82536.1"
                     /translation="MKTTIKYVAPWLAAAAIGGAIALAPIAGAATPSTAGATPPASAG
                     TDPLVPYGTDPHSPYVFGYHVSDHDEANTTNGQLDVPF"
     gene            5581..5904
                     /locus_tag="VFC01_23105"
     CDS             5581..5904
                     /locus_tag="VFC01_23105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZN82537.1"
                     /translation="MIFIVVKFETKPEWTERWPDLVAPFTAATRAEEGNLWFEWSRSL
                     DNSAEYVLVEAFRDGDAGAAHVNSDHFKAAQRDLPKALAHTPKVISQTVDATGWSKLA
                     EITVD"
     gene            6121..6918
                     /locus_tag="VFC01_23110"
     CDS             6121..6918
                     /locus_tag="VFC01_23110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HZN82538.1"
                     /translation="MSPTQQIWRTPPPPPPPSPPEKRRRARGLNWQTIALVVVIVVAL
                     VVGGLAGAELYARHRADSVLTEVAECVVQDGVSISFGVNPPFLWQHITGHYTNISVTT
                     AGNQVQSAKGMTAQVTVKDVRLQDSSDSKGTIGSLNATLSWKSAGIKDTVAANLPGVG
                     NLVTGVSTDAAAGTIILDATGSSVTAKPVVTDGDLNLEVVDVNGLLPKDVVQTGLDGL
                     TKKLNDNYPLGIHADSVKVTDTGVVGTFSSQNASIPKEDANPCFARL"
     gene            complement(6926..7618)
                     /locus_tag="VFC01_23115"
     CDS             complement(6926..7618)
                     /locus_tag="VFC01_23115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate cyclase"
                     /protein_id="HZN82539.1"
                     /translation="MKESTVSLERALDETRTGDIWLFRGTSGPDRAIQTMSNAPVNHV
                     GMTIAIEDLPPLIWHAELGDKLTDMWTGGHHRGVQLHDARQAIERWMTNYHQRCWLRQ
                     LTPFATREQEDRALKVVARMDGTPFPTTARLTGRWFRGRVAVADFTRGIPFVHTRVRE
                     ATQRRKKEKLQVGLETAYCAETVAVTYEEMGLLSTEKHFNWFDPGVFWSGDELPLARG
                     YQLGNEIAVVPG"
     gene            7646..7810
                     /locus_tag="VFC01_23120"
     CDS             7646..7810
                     /locus_tag="VFC01_23120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82540.1"
                     /translation="MRGIWKWVGLAGVAGVVAGGVLVARDQRKRNSYTPEDIRARLHQ
                     RVAKAGDTAT"
     gene            complement(7807..8589)
                     /locus_tag="VFC01_23125"
     CDS             complement(7807..8589)
                     /locus_tag="VFC01_23125"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN82541.1"
                     /translation="MIGAQIAAYVLAGIAAAEAVALVVVWVIYTRQKHELNELRRRVD
                     TRNMLLSGSREAVKQVWQTANILRKDGLGAAVRSSIEDLADWAEVERPDLARLAPSGK
                     VAIVFSDIEESTALNERIGDRAWVRLISSHDKLVRRHVKRHSGHVVKSQGDGFMIAFA
                     QPEQAVLCSIDLQQSLHRRPNGIRVRIGIHMGKSVRRGDDLFGRNVAMAARVAGQADG
                     GEILVSEVVRDAVSDHDIAFDDGRDVQLKGFSGSHRLYAVAT"
     gene            8615..>9490
                     /locus_tag="VFC01_23130"
     CDS             8615..>9490
                     /locus_tag="VFC01_23130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82542.1"
                     /translation="MESQAPYYESRYIRANHPEEPQALWLRETLLLPTAGDPVADVWV
                     MVFDPDGAGNRALKEPYPIDAAAYEYENWTARIGATSIDDRSAHGVVTGGNRSARWDL
                     RITPGSEDAVKLLTDRAYRARIPTAKTTVRHPLAQFDGAVELDETRLVLDGWTGSVNH
                     NWGTRHTPAYAFGQVCGFDDAPDSSLEIVTARAAIGPLLTPAATLFVFRHAGQEFAVR
                     SILGSLQTHGKYKPFSWAFGGRVGDQMIEGEIITEPADVIGLTYTDTDGGSKYCYNSA
                     IANCRVQVAGKAFERR"
BASE COUNT         1728 a         3026 c         3052 g         1684 t
ORIGIN      
        1 gatattgccg gaatcgtcaa aggtgcatcg gaaggcgcag gtctgggcaa caagttcctg
       61 gccaacatcc gcgaggccga cgcgatctgc caagtggtgc gggtcttcac cgatgacgac
      121 gtagcccacg tcgacggccg gatcgatccg aagtccgata tcgaagtgat cgagaccgag
      181 ctgatcctcg cggatctgca gacgctcgag cgcgcggtgc cgcggctgga gaaggaggcc
      241 cgaacccaca aggaccggcg cgcggcgcac gaggccgcgg tggcagcgca ggagattctc
      301 aacggcggca cgacgctgtt cgccgcgcag gtggacgcgt cgctgttgcg ggaactgaac
      361 ctgctgacta ccaagccgtt cctctatgtc ttcaacgccg acgaggccgt gttgaccgat
      421 gacgcgcggg tggcggcgct gcgggagttg gtcgcgcctg cggacgcggt attccttgac
      481 gcgaagatcg aggcggagct gcaagagttg gacgacgagt cggccgccga gttgctggaa
      541 tcgatcggcc agaccgagcg cgggctggat gcgttggcgc gggccggttt tcacacgctg
      601 cggctgcaga cgttcctgac cgcggggcca aaagagtcgc gcgcgtggac gattcaccag
      661 ggcgacaccg cgccgaaggc tgcgggtgtc atccacaccg atttcgagaa gggcttcatc
      721 aaggccgaga tcgtgtcgta tgacgacctg atcggggccg ggtcgatggc cgccgccaag
      781 gccgcgggca aggtgcggat ggaaggcaag gactacgtga tggccgacgg tgatgtcgtc
      841 gagttccgac acggataaac gcgcaagccc tcggaacccg gcaagctacc gatgggctga
      901 tcgtcggtga ggacggttac gagatgtggc ggccgacatc agctggtgaa gcggtcgacg
      961 ctccaattgc agcgtcgtcg gcgacgatgc gccgaatcga acgctaatgg gtcggattta
     1021 gacacggtcg actggcggag acgggcctac cgcgtcgcac cgagatccgt gtcgacggat
     1081 tccacctctt cagcgcgacg cattccgggc gggcgtcttg ccgctgtttg agtttccgtc
     1141 gttcgcattc cgggattcgt cgcgtcgatc gcctttcggg tgtttgtgat tctcagagct
     1201 ggtatcggta ttggtcgcgt ccgtgttcgc cgccctggtg cgtgggttct ccgtcggcga
     1261 tggcgtgtcg tcagatccct tgcctttggt gtcggttttc gaatcttttc gctgggtctg
     1321 cgaggcccgc agatcagcct tggagttcac cggggcaata agggaaggaa cactgtcgac
     1381 ggtgggtgcc gctatcactg cctccggcgt gatagggact atggggggag gaacattccc
     1441 gaaggtgggc gccggaaaca ctgcctctgg cagggcgctg aggccggact tgatgttgtc
     1501 gctgattgtc ttccagctgg tcaagatcaa accaggctct gtcttagggt tggcaccgga
     1561 gccgatcggt ggaggtaaga cagttgcgag ggcaccgatg atcggggcag tcagccggta
     1621 aaggagcgtg tattcgtttc cgaactccgc gtacggctcg ggtaatagca aacagtgcac
     1681 catgaagctc agcagccccg gaatgtcttt cggattcgca acggcggccg cgatcacgtt
     1741 ggccagattt atcggcacgt tgataaagat gaattgcgtg acgagagcga ttgtgcggat
     1801 aatcgcggcg ccgacgacgg gaatgtcgcc gtccagagaa ttgagcgtag ggagatttaa
     1861 cgacgccgag attaacgggc caggcacgtt cacctggccg gatatccgcg cgctcaaaat
     1921 acccgggttc ctactcggat cgaggctggc ggtttgcggc gggcgggcaa tgtccgattc
     1981 cgccccaccc gccgcgtggg tcggataatt ttgccataga agagatggtc cggtgccgtc
     2041 cgacagatct gctgtcagga actgcaactt gaaggcgatt gcgggttggg aaggtatcaa
     2101 tctgtgcgcc ggcaggtgca ccgatgtcgc gaccagtacg cttgctcccg cgatggcgac
     2161 cccggtcgta atatatcggc gaagcatgac gtgcatcgtt ggcctcctcc gcgccgggat
     2221 gctccgcaaa accctagagc aaatgggaag ctcctgaggc gagattgtca atactccgca
     2281 atattgacaa cacggtcaat gtcgagttaa cgcaatagag tttcttcagt ccgatcggcg
     2341 tggccagcct gaaccgccct cgcatgtccg gagactccca atacttggaa gtggagtctt
     2401 gtcagtggtc gaggagctac ccgccgcagc tgcgggggtg tgcggttcgg atggtcgacg
     2461 ccgattagcg gttcggcttt ggagtccggt actcgagcat cgggtcggta agaactgcgt
     2521 cgccagacat cgcgactaac tccgtcgcgg aaatcaggta tttggcgccg tcgccacgcg
     2581 cttcgaatcc ttgtgctgtc actcgcacat cgtcaagttg cagcgcggcg cgatcgctca
     2641 agcgcattcc gttgtactgg taacggttgt tgggaccctt acacacgacg acgaccgacc
     2701 gattggtgcg cccgatggca actgcactct gattgccttc gcaccgggcg ttggagttga
     2761 cgaagccgct gtcgtccatc gcgatgggag gtgcgacagg cggtttggcg ggcagggtcg
     2821 ttcgatcaac ggccggtttg accggctgag tcgttcccgc cgcggctggc ttcggttgcg
     2881 ccgctgttgg atacggttgc gccgcagttg gcaagggggc ccgtgcgctt ggcgtcgagc
     2941 cgggctcgtc catcaccaac gttgcaacga ccacgcccgc agtcgcgacc gacgcggcca
     3001 ccaccccaac gacagccgct ttgccgctaa gcatgcgctg agaccggggc gcctctgacg
     3061 gcggttcttc gatcggttct gctcgctctt gctcgtcgtc cgttggcata gtccatagct
     3121 cttgcagctg ataccagtca gtcaacacgg gcggcccctc ggtcgcagct cgaacgagcg
     3181 cgcatgcact cacccgtagc aaaacacctc aatgtcgaat accggtgatt tggcgcggcg
     3241 tttcgccgac gatggttgcc gacattgcct gccggcacag gtttcggcga gtagtgtgac
     3301 gtcgacccag cgtatgcacg caggccagga cggtggtgac gaccatgtat ccgaggcgca
     3361 tcgcagggct cgtagcgtgc gtcgtcgcgt tgatcgcagg ctgctcgtcg acgaccgagt
     3421 cgtccaccgc atcctcggct acgccgaccg cgacgagcgc cgcaccatcg aagaattccg
     3481 cgcttaagac catcgatccg aaggcatttc aggccgtcgt cgacaccgcc gcaaagcagc
     3541 tgatggtccc cggagcgatg gtgctgctgc gcaccccgca gggcaacttc agcgctacat
     3601 acggcaccac cgagctcggc gcgcagacac cgccagcacc gaacacgcat ttccggatcg
     3661 cctcgaacac caagaccatg actgcagcgc tgatcgtgct gctcgcccag gacggcaagc
     3721 tgaagttcag cgatccggtg tcaaactacg tgcctggtgt gcccaacggc gaaaacatca
     3781 ccgtcgcaga gcttttgaag atgcgcagcg gcctctacgg ttactcggcc gaccctgcgc
     3841 ttgccgccgc gatggatgcg aacccgggca aggcatggac gcctcaagag gtactggata
     3901 tcgcgttccg gcatccgccg cagttcgcgc ccgacgcctc ctacgagtac agcaacacca
     3961 actacgcgct gctcggtctg atcgcggaga aggtgggtgg acaaccgctg cgccagcagc
     4021 ttgccgagcg gctattcgga ccggtcggcc tcgcgcagac gtcacttccc gctacggacg
     4081 acacgaccat cccagcgccc tactcacacg gctacatgta cggcggcagt tactacgcac
     4141 tcgccgacga cccgtatccc gcccagatgc aggccgccgc gcgggccgga acgttgaagc
     4201 cgatcgacta caccaaccag aactcttcgt acgccaccgc cgccggcgga gcgatctcga
     4261 cggcggatga cctcgccacg tggatgaaag cgctcgtctc gggcaaggtc ttcaacgccg
     4321 actaccacca gcagtggttg acgagcctgc aggccgagga tccggacgcg cccgacggac
     4381 agaaatacgg ctacggaatc tcatatcaac gtttcggtcc aaacgccgcg atgttctacc
     4441 acggaggaga acttccgggc ttcaattcgt tcatgggcta cgacccggat aacgatgtgg
     4501 cgctggtcat ttggacgaat ctgactctct cgccggacgg caagacgacg gcgcaggcga
     4561 tgttggccac tattctcgac gagatctacg cgggcttgtc gctggcgtcc ccgccggctc
     4621 cgacaaccac gaccacgcgg tgacggcacg gaattccgct gaagcggagc gcgtgcgatg
     4681 gatcagaaag gcacgtccac atcgtcgccg agccacttgt ggtaaccgaa gatcgccggg
     4741 ctctcagggt cggtgccgta cggggtggcc ggatcgatgc cggtgtcggc gtttggcgaa
     4801 ggggtgaccg cttgcgtcac aggtgccgga tgggtagcgg cgctggcgac gggggccaca
     4861 gcaatcgcgg caccaatggc agctgcggcc aaccacggtg tggcgtactt gacggttgtt
     4921 ttcacgatgt tctcctgtcg gactagacag ccatgattgg acaaccaatc atggcttgat
     4981 ggcaaacggt tgtcgccacc acttgcaccc attcaactgt tgctgctagc tggaattcaa
     5041 agatcagctg gttccgccag caatctttcc ctaaagccgt agaccccatc tgcgtggcaa
     5101 ctgtgcagag ggggtccggc gttcggcgcg gggtgtctca gaagggaacg tccagttggc
     5161 cgttcgtggt gttggcctcg tcgtggtccg aaacgtggta gccgaacacg tacggagagt
     5221 gtggatccgt gccgtacgga acgagtggat cggtgcccgc gctggcgggt ggcgtggcgc
     5281 cggctgtcga cggggtcgcg gcgccagcga tgggtgcgag ggcaatcgct ccaccgatcg
     5341 ctgctgcggc cagccacggt gcgacatact tgatcgttgt cttcatgatg tttccccttt
     5401 gctggagcgc tctgtttggt ctatgaaaat catcgcgctc tagcaagcgg gtgtcgtcaa
     5461 cgctggcact cctttggacc tgcagctagc tggaatctgc cagttccagg ccgctgcaac
     5521 cctctaccgc tgcagccagc gacgcgctga gacatgatgg gtgtgcggga gggagcccta
     5581 atgatcttca tcgtcgtcaa gttcgaaaca aaacccgagt ggaccgaacg ctggcccgac
     5641 ctcgtcgcac ctttcaccgc ggccacgcgc gctgaggaag gcaacctctg gttcgagtgg
     5701 tcgcgcagcc tcgacaactc ggcggagtac gtcctcgtgg aggcgttccg cgacggcgac
     5761 gccggcgcag cccacgtcaa cagtgatcac ttcaaggccg cacagcgcga cctaccgaag
     5821 gcgctcgcgc acactcccaa ggtgatcagc cagaccgtcg atgccaccgg ttggtcgaaa
     5881 ctcgccgaga tcaccgtgga ctgagcgacg gtatgaggca gaatcgcgca agtgacgccg
     5941 ccgcaacgcc ccgggcaccc tgattggcgc ggtagggcca ctccaccgcc cggcccggcg
     6001 ggccgtcggc cgccgcctcc cggacgcccg gagccgccga ctgaacacat tcgcggcccc
     6061 taccgacccg cagacgcagg ggccacccgc aggatcgctc gcccgcagtc acctgcggat
     6121 ttgtcgccga cccaacagat ttggcggacg ccgccgccac caccgccacc atcgcctccc
     6181 gagaagcggc gccgcgcccg cggtctcaat tggcaaacga tcgcgctggt cgtcgtcatc
     6241 gtggtcgcac ttgtggtggg cggactggcc ggcgccgagc tgtacgcccg gcaccgtgcc
     6301 gacagcgttc tgaccgaggt cgccgaatgc gtcgtccagg acggtgtctc catctcgttc
     6361 ggggtgaatc cgcccttcct gtggcagcac atcaccggcc actacacgaa catctcggtt
     6421 accaccgccg gaaaccaggt tcaaagcgcc aagggaatga cggcccaggt gaccgtcaaa
     6481 gacgtgcggc tgcaggattc ctcggactcc aagggcacca tcggttcgct caacgcgacg
     6541 ctcagctgga agtctgcggg catcaaggac accgtggccg ccaacctgcc gggggtgggg
     6601 aatctggtga cgggcgtgag cactgacgct gccgcgggca ccatcatcct ggacgccacc
     6661 ggcagcagtg ttaccgccaa gcccgtcgtg accgacggcg acctgaacct cgaggtcgtc
     6721 gacgtcaacg gcctcctgcc gaaggacgtc gtgcagacgg ggctggacgg cctcaccaag
     6781 aagctgaacg acaactatcc gctcggaatc cacgccgaca gcgtcaaagt gaccgacacc
     6841 ggagtcgtcg gaacattctc cagccagaac gcctcgatcc cgaaagagga cgccaacccc
     6901 tgcttcgccc gcctctgagc gccaattaac cggggacgac ggcgatttcg ttgcccagct
     6961 gatacccccg cgccagcggc agctcgtcgc cgctccagaa cacgccggga tcaaaccagt
     7021 tgaagtgctt ctccgtcgaa agcaagccca tctcctcgta ggtgacggcg acggtctccg
     7081 cgcaatacgc cgtctccaac ccgacttgga gcttctcctt cttgcggcgc tgggtagcct
     7141 cacgaaccct ggtgtgcacg aacggaattc cccgcgtgaa gtcggcgacc gccacgcggc
     7201 cgcggaacca ccgcccggtg agccgggccg tggtcggaaa cggcgtgccg tccatccgtg
     7261 caacgacctt gagcgcgcgg tcctcttgct cgcgggtggc gaacggcgtg agttgacgca
     7321 gccagcagcg ctgatggtag ttggtcatcc acctctcgat agcctgccgc gcgtcgtgca
     7381 gctgcacgcc gcggtgatgg ccgcccgtcc acatgtcggt cagtttgtcg cccagctcgg
     7441 cgtgccagat cagcggcgga aggtcctcga tcgcgatcgt catcccgacg tggttgaccg
     7501 gggcattgct catcgtttgg atcgcccggt cggggccgga ggtgccgcga aacagccaga
     7561 tatcgccggt cctggtctcg tccagcgccc gctcaagcga gaccgtgctt tctttcaccc
     7621 ccgcaggcta aggcagactc gtcgcatgcg tggcatatgg aagtgggttg gactggccgg
     7681 tgtcgccggt gtggtggcgg gcggcgtgct ggtcgcgcgc gaccaacgta agcgcaactc
     7741 atacacgcca gaggacatcc gcgcccgcct tcaccagcgg gtggccaagg ccggcgacac
     7801 ggcgacctaa gtggccacgg cgtagagccg gtgcgagccg ctaaagccct tcaattggac
     7861 gtcacggccg tcatcgaagg cgatgtcgtg atcgctgacg gcgtcgcgca ccacctcact
     7921 gacgagaatc tcgccgccgt cggcctgtcc ggccacccgg gccgccatcg cgacgttgcg
     7981 gccgaacagg tcgtcgccgc gacgcaccga cttgcccatg tggatgccga tgcggacccg
     8041 aatcccgttg ggccgccggt gaagtgactg ttgcaggtcg atgctgcaca gtaccgcctg
     8101 ctcgggctgt gcgaaggcga tcatgaagcc gtcgccctgg ctcttcacca catggccgga
     8161 gtgcctcttg acgtgcctgc ggaccagctt gtcgtggctg ctgatcagcc tgacccacgc
     8221 ccggtcgccg atgcgctcgt tcagcgccgt cgactcctcg atgtcggaga acacgatcgc
     8281 caccttgccg ctcggcgcca gccgcgccag atccggtcgc tccacctcag cccagtcggc
     8341 gagatcttcg atcgacgacc ggaccgccgc gcccaggccg tccttgcgga ggatgttggc
     8401 ggtctgccag acctgcttga cggcctccct gctgccggag agcagcatgt ttctggtgtc
     8461 gacccggcgc cgcaattcat tgagttcatg cttctgacgt gtgtagatca cccaaaccac
     8521 gacgagtgct actgcttccg ccgcggcgat ccccgcgagc acatatgccg caatctgcgc
     8581 tccgatcacc gctcaagtat tggatgctga tcccgtggag tcccaggcgc cctactacga
     8641 gagccgctac atccgcgcca accaccccga agaaccgcag gcgctatggc tgcgggaaac
     8701 cctgttgcta cccacggcgg gcgatccggt ggccgacgtc tgggtgatgg tgttcgatcc
     8761 cgatggcgcg ggcaaccggg cccttaagga gccctacccg atcgatgccg ctgcatacga
     8821 gtacgagaat tggacggcgc gcatcggcgc gacatcgatc gacgaccgat ccgcgcacgg
     8881 cgtcgtgacc ggcggaaacc gttcggcccg ttgggatctg cggatcactc ctggatccga
     8941 ggatgcggtc aagctcctca ccgaccgcgc atacagggcg cgcatcccga cggccaagac
     9001 aacggtgcgc catccgctgg cccagttcga cggggcggtg gagctcgacg agacgcggct
     9061 ggtgctcgac ggctggaccg gcagcgtcaa ccacaactgg ggcacgcggc acaccccggc
     9121 ctacgcgttc ggccaggtgt gcggcttcga cgacgcgccc gactccagcc tggagatcgt
     9181 gacggcacga gccgcgatcg gaccgctatt gacgcccgcg gcaacgctgt tcgtgttccg
     9241 ccacgcgggt caggagttcg cggttcgctc gatcctgggc agcctgcaga cgcatggcaa
     9301 gtacaagccg ttctcgtggg cgttcggcgg gcgggtgggg gaccagatga tcgagggcga
     9361 gatcatcacc gagccggccg acgtcatcgg cctgacctac accgacaccg acggcggctc
     9421 gaagtactgc tacaactcgg ccatcgcaaa ctgccgagtt caagtggcgg gcaaggcatt
     9481 cgaacgcagg
//
LOCUS       DASRJA010000529         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3155566,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000529 DASRJA010000000
VERSION     DASRJA010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3155566"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VFC01_23135"
     CDS             complement(<1..1239)
                     /locus_tag="VFC01_23135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN82543.1"
                     /translation="MSGTDWSDVVMSELPTGTVTLLLADVEGSTRLWESQPDEMTAAI
                     ARLDHVLTDLTAAHEGVRPVEQGEGDSFVLAFARASDAVACAVALQRAPLAPIRLRIG
                     LHTGEVRLRDEGNYIGPAINRTARLRDLAHGGQTVLSGTVEDLVVDSLPADAWLTDLG
                     THALRDLPRPERVVQLCHPDLRNEFPPLRTPKTVGTHNLPAQLTSFVGRQAQMVDVRT
                     LLADNRLVTLTGAGGAGKTRLAIEISARVAPQFPSGVWYVDLAPTTYAEVVPVVVARA
                     LGLPDQPDTSTPDTLVRFVADRETLLVLDNCEHLIDATASLATNLLGACPALRVLTTS
                     REPIGVAGEVTFLVPSLSLADEAVELFADRARRVRPDFGVTDDNAATVTEICRRLDGM
                     PLAIELAAARVRALSLDEILG"
     gene            complement(1331..2542)
                     /locus_tag="VFC01_23140"
     CDS             complement(1331..2542)
                     /locus_tag="VFC01_23140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN82544.1"
                     /translation="MKALVFGVPPEPFEVADALARSPVALREVPDPQPLHDDWVITRP
                     RLTGICGSDSKQILLDFGEGDTDNAMAAFCSFPQVMGHEVVADVVALGPAARGLEVGQ
                     RVVLNPWLSCGPRGIEPPCPACETGDYSLCWSFTDGDIKPGIHTGVSADVTGGYAELM
                     PAHDSMLFAVPELVPDELAVFADPFSVSLHAITRHPPPPSGRVLVYGAGSLGLCAVAV
                     LRALYPEVAVAVVARFGAQAELARRFGAERVLAHEPRLAVIEDLVGWGGGRLRQPLQG
                     LPMAYPGAIDVVYDTVGKPETFEVGVRVLKARGTLVKAGVHAPGRWEWSPLYFKEINW
                     VGSNAFGVEEVDGQRKHAIAHYLDLVVDGRVDLRPMLTHTFRLEQWRDAFLAIANQGE
                     SGAVKVAIDFR"
     gene            complement(2539..>2954)
                     /locus_tag="VFC01_23145"
     CDS             complement(2539..>2954)
                     /locus_tag="VFC01_23145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN82545.1"
                     /translation="LTLAVLPKMLERGDGMVVNVSSVGGRLGIIHESAYCASKFALCG
                     WSESMAVDLHGTGVSVKLIEPGPVDTEIWDQPDNDEPIYDGPKFSPEEVAVGIIDALG
                     SDGFEHYVPDMKAVVDLKNADIDTYIAGAAGMAQS"
BASE COUNT          561 a         1031 c          902 g          460 t
ORIGIN      
        1 gccaaggatc tcgtcgagcg acaaggcccg cacccgtgcc gccgccagct cgatggcgag
       61 cggcatgccg tcgagccggc ggcagatctc ggtcacggta gcggcgttgt catcggtaac
      121 gccgaaatcg ggccgcaccc ggcgggcccg gtcggcgaac aattcaacgg cttcgtcggc
      181 aagcgatagc gatggcacaa gaaacgtcac ttcaccggcc acgccaatgg gctcacggct
      241 cgtggtgaga acccgcaacg ccggacaggc gcccaagagg ttagtcgcta gggatgcggt
      301 cgcgtcgatc aagtgttcgc agttgtcgag cacaagcagc gtctcccgat cggcgacgaa
      361 ccgcaccagc gtgtcgggcg tcgaagtgtc cggttgatcg ggcaggccga gcgcacgggc
      421 caccacgacc ggcactactt cggcatatgt cgtcggcgcc aagtcgacgt accagacgcc
      481 gctgggaaac tgcggcgcta cgcgagccga gatttcaatg gcgagacgcg tcttgcccgc
      541 accaccggca ccggtcaacg tcacgagccg gttgtcggcc aacaaagtcc gcacatccac
      601 catctgcgcc tggcggccaa caaagctggt cagctgcgcc ggaaggttat gcgtaccaac
      661 agttttaggc gtgcgcaatg gcgggaactc gttgcgcagg tcgggatggc atagctgtac
      721 aacgcgttca ggccgtggca agtcgcgcaa ggcatgcgta ccgagatcgg tcaaccaggc
      781 gtcggccgga agggagtcca cgaccagatc ctcaacagtg cccgagagca ccgtctgacc
      841 cccatgcgcg agatcacgca agcgcgcggt cctgttgatc gccgggccga tgtagttgcc
      901 ttcgtcgcga agccgcacct cgccggtatg cagtccgatc ctcaaccgga tcggcgccaa
      961 cggtgcccgc tgcaacgcga ctgcgcacgc caccgcatcg ctggcgcgag cgaatgcaag
     1021 tacgaaacta tcgccctctc cctgttcaac cggcctgacc ccttcgtgcg cggcggtcag
     1081 atcggtcaat acgtggtcga gacgtgcaat ggcggcggtc atctcatcgg gctgtgattc
     1141 ccacagccgc gtcgagcctt cgacatcggc gagcagcaac gtcaccgttc cggttggtag
     1201 ctcgctcatg actacgtcgc tccagtcagt tccgctcatg ctagccagca tgcggaggcc
     1261 acgagtccca aacatcagcg aaaacccgta tacggctcac cctcaccccg caaatccgag
     1321 cggagccaag ctagcgaaag tcgatggcca ccttgactgc gccggactcc ccctgatttg
     1381 cgatcgcaag gaaggcgtcg cgccactgct ccagccggaa cgtgtgcgtt agcatggggc
     1441 gcaggtcgac tcggccgtcg acgacgagat cgaggtagtg cgcgatcgcg tgcttgcgct
     1501 gaccgtccac ctcctcgaca ccgaacgcgt tggacccgac ccagttgatc tccttgaagt
     1561 acagcggact ccactcccac cgccctggcg cgtgcacgcc cgccttcacc agcgtccctc
     1621 gcgccttgag aacgcgcacg ccgacttcga acgtctccgg cttgccgacg gtgtcataga
     1681 cgacgtcgat cgcgccggga taggccatcg gcaacccctg caacggctgt cgcagcctgc
     1741 cgccgcccca cccgacgagg tcctcgatga cggccaaccg cggctcatgc gccaacaccc
     1801 gctccgctcc gaaccgacgg gccaactccg cctgcgcgcc aaaccgcgcc acgacggcca
     1861 ccgccacctc tgggtacagc gcgcgcaaca ccgcgaccgc acataaccca agggagcccg
     1921 ccccgtacac gagcacccgg ccagacggcg gcggcggatg ccgggtgatc gcgtgcagcg
     1981 acaccgagaa cgggtcggcg aacaccgcga gctcgtcggg caccaattcc ggcaccgcga
     2041 acagcatgct gtcgtgggcc ggcatcaact cggcgtagcc acccgtgacg tcggccgaaa
     2101 cgccggtatg gatacccggc ttgatatcgc cgtcggtgaa gctccagcac aagctgtagt
     2161 caccggtttc gcatgccgga caaggcggtt cgatgccgcg cggaccgcac gacagccacg
     2221 ggttgagcac gacgcgctgc ccgacctcca gcccgcgcgc cgcaggaccc aaagccacga
     2281 catcggcgac gacttcgtga cccatcactt gggggaacga gcagaatgcc gccatcgcgt
     2341 tgtcggtatc gccctcaccg aagtcgagca agatctgttt ggagtccgat ccgcaaatcc
     2401 cggtcaggcg cggccgggtg atcacccaat cgtcgtgcag cggttgagga tccggtactt
     2461 cgcgcagcgc gacgggcgac cgcgcgagag cgtcggcaac ctcgaacggc tcgggcggca
     2521 ccccgaatac cagcgccttc atgactgggc catgcctgcc gcgccggcga tgtaggtgtc
     2581 gatgtcggcg ttcttgaggt cgacgacggc tttcatgtcc ggcacgtagt gctcgaagcc
     2641 atcgctgccc aacgcgtcaa tgatgccgac agccacctcc tcggggctga acttgggacc
     2701 gtcgtagatg ggttcgtcgt tgtcgggctg gtcccagatc tcggtgtcga cgggtcctgg
     2761 ctcgatcagc ttcaccgaga cccccgtgcc gtgcaggtcg acggccatcg attcgctcca
     2821 tccgcacagc gcgaacttgc ttgcacagta agcggattcg tgaatgatcc cgagcctacc
     2881 gccgacactg gacacattga cgaccatccc gtccccgcgc tccagcatct tcggcagcac
     2941 ggccaacgtc agcc
//
LOCUS       DASRJA010000530        23662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3125174,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000530 DASRJA010000000
VERSION     DASRJA010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 23662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23662
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3125174"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..903)
                     /locus_tag="VFC01_23150"
     CDS             complement(91..903)
                     /locus_tag="VFC01_23150"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZN82546.1"
                     /translation="MTPDLIASDVDGTLLDDDEKITARTRDAVHAAVASGAQFVLATG
                     RPPRWVQPVVDALGFAPMAVCANGAVIYDPATDRIISARTLSADALGELAEVATRVID
                     GAGLAVERVGNSAHDAATPQFVSSPGYEHAWLNPDNTEVSIEDLLSAPAVKLLIRKAG
                     ARSSDMAAALAKHVGLEGDITYSTNNGLIEIVPLGISKATGVEELARPLGITAESVVA
                     FGDMPNDVPMLHWAGLGVAMGNAHPDAVAAADEVTASNVEDGVARVLERWWL"
     gene            complement(900..1664)
                     /locus_tag="VFC01_23155"
     CDS             complement(900..1664)
                     /locus_tag="VFC01_23155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN82547.1"
                     /translation="MEPVFRTLEIAVSLATKAIGTKITYQGLENIPDRGGAVIAINHT
                     SYVDWWPAALAAYQRHRRMRFMIKTEMQDVKIVNFLIKHSGTIPVDRTSGGGAYAVAV
                     ERLRAGEIVGVYPEATISRSFELKEFKSGAARMARDAEVPIVPLIVWGAHRRWTKDHP
                     RDMGRRSKIPVTVAAGPPLDAAETTDETSAAMREGMTTLLRTVQEEYPHPEGAYWVPC
                     RLGGSAPTMDEAKALEEAELAERARKRAEREAKSHR"
     gene            complement(1670..2446)
                     /locus_tag="VFC01_23160"
     CDS             complement(1670..2446)
                     /locus_tag="VFC01_23160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HZN82548.1"
                     /translation="MEPVYGTVIQLARLVWRAQGLKFTVTGVENLPRTGGAVVAINHT
                     SYFDFTFAGLPAYKQHLGRKVRFMAKKEVFDNKVTGPIMRSLRHIEVDRDSGAASFDH
                     ACQALKEGELVGVYPEATISRSFEIKEFKSGAARMAVAADVPIVPHIVWGAQRIWTKG
                     HPKKMWRPKVPISVAVGEPIMPTLPPTELTALLHSRMQHLLERVQDEYGPYPPGEYWV
                     PHRLGGGAPTLAEANRMDAEEAAEKAARREQRTDPTGAPG"
     gene            2465..3148
                     /locus_tag="VFC01_23165"
     CDS             2465..3148
                     /locus_tag="VFC01_23165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015291755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="HZN82549.1"
                     /translation="MTAPSEPVPVPETVERIAAGRTVVAVWINELGGVTYAVDGGCEY
                     VKVAPSEWAHHLVAEVARLRWAAAYVRVPRVIGSGDGWLHTAGLPGRSAVDPRWVSDP
                     RTAARAIGAGLRMLHDTLPVDDCPFGPPPWVTDSLPADRLVICHGDACAPNTLIDDDG
                     RCCGHVDVGDLGVADRWADLAVATLSLSWNYGGSWEGDLFDAYGVEPDTTRIAYYLGL
                     WNAGDISSR"
     gene            3187..4731
                     /locus_tag="VFC01_23170"
     CDS             3187..4731
                     /locus_tag="VFC01_23170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN82550.1"
                     /translation="MQVTSVGHAGFRIDTKAGSILCDPWVNPAYFASWFPFPDNSQLD
                     WAALGDVDYLYVSHLHRDHFDPKNLRDHVNKDAVVLLPDYPVPDLQRELEKVGFTRFF
                     ETTDSVKHRVSGPKGDLDVMIIALRAPADGPIGDSGLVVDDGETVCFNMNDARPVDLD
                     VLHADFGHVDVHMLQYSGAIWYPMVYDMPARAKEAFGTQKRQRQMDRCRQYIAQVGAT
                     WVVPSAGPPCFLDPELRDLNDDHGDPANIFPDQAVFLDQMRTHGHDGGLLMIPGSTAD
                     FTGPQLNSLTHPMPDPESILTTGKAAYIADYAERMAPVLAAEKASWAPASGEPLLEPL
                     RIVLEPIMLQSDQICDGIGYPVELRLGAETVVLDFPKRVVREPIPDEKFRYGFAIAPE
                     LVRTVLRDNEPDWVNTIFLSTRFSAWRVGGYNEYLYTFFKCLTDERIAYADGWFAEAH
                     DDTASIMLDGWEIQRRCPHLKADLSKFGVVEGSTLTCNLHGWQWNLDNGRCLTTKGHE
                     LRSERK"
     gene            4728..5063
                     /locus_tag="VFC01_23175"
     CDS             4728..5063
                     /locus_tag="VFC01_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82551.1"
                     /translation="MTAPRQYYDDGLVQLDREAITLRRYHFPSGTSKIIPLRSIRGYK
                     AESLGLITERFRIWGSTDPRRWMPLDVWRPIKSTLVTLDVPGVRPSPAFTPQRPKEFL
                     ARLDELLND"
     gene            complement(5329..6588)
                     /gene="serS"
                     /locus_tag="VFC01_23180"
     CDS             complement(5329..6588)
                     /gene="serS"
                     /locus_tag="VFC01_23180"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZN82552.1"
                     /translation="MIDLKVLRENPDLVRASQRARGEDPGLVDALLEADAARRSAVSA
                     ADNLRAEQKAVSKQVGKASPDERPALLEQAKAFADKVKASEAEQAEAEKAFTAAHMTI
                     SNVIIDGVPAGGEDDFVVRETVGEPRAIENAKDHVELGESLGLIDLERGAKVSGSRFY
                     FLTGAGALLQLGLLQLAVRLATENGFTLVIPPVLVRPEVMSGTGFLGAHAEEVYRIEA
                     DDLYLVGTSEVPLAGYHADEILDLSDGPLRYAGWSSCFRREAGSHGKDTRGIIRVHQF
                     DKVEGFVYCKPADAEAEHERLLDWQRQMLAHIEVPYRVIDIAAGDLGSSAARKFDCEA
                     WVPTQQTYRELTSTSNCTTFQARRLSVRYRDENGKPQTAATLNGTLATTRWLVAILEN
                     HQQPDGSVRVPDALVPYVGTDVLKPKR"
     gene            6701..8146
                     /locus_tag="VFC01_23185"
     CDS             6701..8146
                     /locus_tag="VFC01_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HZN82553.1"
                     /translation="MERMLDAPEREDLLADSHKSDDGRRVAWWQSFHATSTRRALLLT
                     ALGGLLIAGLITALPSTDGSNGLTANSISLGPRGNDTFNHANSGDCLNWPDRTPDAGE
                     IVDCTGDHRFEVAESVDMRTFPGSEYGPDAAPPSPARIQQISQEQCSAAVKRYLGARF
                     DPNSRFTVSMLWSGEKAWRQSGERRMLCGLQLPGPNNQQLAFKGKVADVDQSKVWPAG
                     TCLGIDPSTNQPTDIPVDCAAPHAMEVTGAVNLAEKFPQALPPEPEQDGFIKDACTRM
                     TDAYLAPIQLRSTTLTLIYSTISLPSWSAGSHQVSCSIGATLGNGGWSTLLNSAKGPL
                     QINGQPPIPPPDIPEERLNLPPIPAMEPQMPDDDSSYSSSSQATNSDTTDHGSQQTGT
                     QHMPGQQSTGQTGTSTVPAPPPPGNTFLNGPPPPGAPLDAPPPPEGAPPPPPGAPPGP
                     APAPPPAEVAPAPGPPPPPGDPLLLPPPPGQ"
     gene            8151..8501
                     /locus_tag="VFC01_23190"
     CDS             8151..8501
                     /locus_tag="VFC01_23190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HZN82554.1"
                     /translation="MTVRMSPQRFDELVSDALDLIPPELAAALDNVVVLVEGRNPDEP
                     EILGLYQGVALTERDSWYAGSLPDTITIYRDALLEVCEDEAEVVDEVAITVIHEIAHH
                     FGIDDDRLHELGWG"
     gene            8548..8742
                     /locus_tag="VFC01_23195"
     CDS             8548..8742
                     /locus_tag="VFC01_23195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82555.1"
                     /translation="MSSECRDCLAGLEHCHGTVIHHAWYRTECTEDGCVTPEVVHLFS
                     IDCEAIGCTCDAAVSAHRVG"
     gene            complement(8722..9408)
                     /locus_tag="VFC01_23200"
     CDS             complement(8722..9408)
                     /locus_tag="VFC01_23200"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZN82556.1"
                     /translation="MSGRLVLVRHGQSHGNVERRLDTRPPGAALTDLGRDQARAFARE
                     LMHPAAMIAHSVAVRAEQTAAEISGALGLDTHEFEGIHEVQVGDLEDRNDDDAIAEFE
                     TVYQRWHEGDLDVPMPGGETGNEVLDRYVPVLTQLRMRYLDDDAWHGDIVVVSHGAAI
                     RLVSAVLAGVDSSFALDHHLPNAESVVLSPITDGRWSCVQWSTLTPPFYPEPDVHPVE
                     DALSSADPMG"
     gene            complement(9405..10313)
                     /gene="pheA"
                     /locus_tag="VFC01_23205"
     CDS             complement(9405..10313)
                     /gene="pheA"
                     /locus_tag="VFC01_23205"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HZN82557.1"
                     /translation="MPHIAYLGPEGTFTEAALRGMVAANLVPLSDFEPFPASSPSAAL
                     EMVRAGDATYACVPIENSIDGSVLPTLDNLATGSPMQVFAEFTLDIGFTIAVRAGVTR
                     PASIAAYPVARAQVAKWAAENLREAEFHPADSNAAAAVDVAEGRADAAVTTALAAKRY
                     GLTALATDVVDEPNARTRFVLAGQPAPPPSRTGADRTSVVLRLDNVPGALVSALTEFA
                     IRDIDLTRIESRPTRKELGTYFFFLDCVGHIDDEAVAEALKALHRRCADVRYLGSWPT
                     GSAAGAAPPHLDEASRWLTRVREGKP"
     gene            10428..11189
                     /locus_tag="VFC01_23210"
     CDS             10428..11189
                     /locus_tag="VFC01_23210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HZN82558.1"
                     /translation="MTAAAPTTAERIRSACVRAGGAMLAVEGAEPTSTPVHHLLDDGS
                     FALTVPRDGMLAGMTVAAGAEGVQAVLEMTDYAPLPLREPVRSLVWIRGRLHGIPDSE
                     VAALLDLIAEEDPNPALLQVNSESTLVRLEIESVVVADSTGAESVGVSALLGARPDPF
                     CAMESCWLKHMESAHREVVDRLASRLPMPLRRGRVRPLGLDRYGVQLRVEGDDGDHDV
                     RLPFAKPVDDVTGLSQAIRILMGCPFLNGLRARRL"
     gene            11217..11984
                     /locus_tag="VFC01_23215"
     CDS             11217..11984
                     /locus_tag="VFC01_23215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HZN82559.1"
                     /translation="MTGPPDELTDPQRRVIRIEIVVVLAVSFGLSAYTAFLNLIEAVL
                     LGLAGQRVALNPRRSPFDLIDLGLNLAVAFQLVAWGALGVYLLWRSGFGPAQIGLGRV
                     RWRPDVLGGLGLAALIGLPGLGLYVAARALGLSAHVEPVELYDTWWRIPVLLFTAFAN
                     GWAEEVIVVAFLLTRLRQLRVNPVTALLASSLLRGAYHLYQGFSAGLGNVVMGLVFAY
                     VWRRTGRLWPLIIAHGIIDSVAYVGYALASGHLGWLS"
     gene            11994..13190
                     /locus_tag="VFC01_23220"
     CDS             11994..13190
                     /locus_tag="VFC01_23220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZN82560.1"
                     /translation="MSDNRPPPRGHDPRVRREPSQVIRRDPNYRPPPGWPPNPPTPAR
                     RPPPPPPPPRRPPPVPPRQQHQPPPPPKAKKPRRKRHWGRIVLAFLFVFVVAVVAGGV
                     WMDSSLHRIPALADYPERPATGTGTTWLLVGSDSRQGLTPEQQAELATGGDIGNGRTD
                     TILLVHVPAIGSSTPTTMVSIPRDSYLPIPGYGEDKINAAFSEGGAPLLAQTVEQATG
                     LRLDHYAEIGFDGFAVMVDAVGGVTMCPTEPISDPLAGIDLPAGCQKLDGRSALGYVR
                     TRATPRADLDRMINQRQFMSALLHRAASPAVLLNPLRWYPMAHAASGALTVNEGDHIW
                     DLARLAWALRGDVTTTTVPIGEFTGGDSGSVVVWNSDVAGQLFSALASDSPVPQEVLD
                     AADNGS"
     gene            complement(13187..14011)
                     /locus_tag="VFC01_23225"
     CDS             complement(13187..14011)
                     /locus_tag="VFC01_23225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HZN82561.1"
                     /translation="MNSDDGTLGRHPFVVAHRGASADRPEHTSAAYELALKEGADGVE
                     CDVRLTRDGHLVCVHDRRVDRTSNGTGLVSEMTLAQLRELDFGAWHPSWRLDGSHGDT
                     GLLTLDDLVSLVLDWNRPVKMFIETKHPVRYGALVESKVLALLHRYGIASPASADLSR
                     AVVMSFSAAAVWRIRRAAPMLPTVLLGETSRYLGGSAATTVGATAVGPSIATLREHPE
                     LVDRAAAQGRALYCWTVDHYEDVRYCRDVGVAWVATNHPGRTKAWLQDGLTGAGRD"
     gene            complement(14016..15095)
                     /locus_tag="VFC01_23230"
     CDS             complement(14016..15095)
                     /locus_tag="VFC01_23230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HZN82562.1"
                     /translation="MIQVCSQCGTRWNVRDRRRVWCPRCHGALLAPSGPAPGAEWSAH
                     PAASPAGPGGLGSPPAGGGRRPALPPGYRWIAVRPGAPPPPRRRRGALGPTPRYAVIP
                     RWGLVDQFEAPEQQTVGLRTGPSLTGVRATLIATMVVFGVAALVHLLRYALLIVNRSI
                     LLNKVVAFTATWLGVLVSVVAVFMIVATAVVLTNWLIARRAAAFAHHRRPDPRPVWAL
                     RAGCLVPLVNLFWAPVFVIELADVEERLKWLGRPITVWWVVWILSTAVSGFSIATSFT
                     QDPQGIADNTVTTIVAYLMALAALLLVMKVFFGFERQPVERPAKRWVIVREDQKDDAR
                     TAEEQPESEPERAAPVESGGQNPAA"
     gene            15753..15911
                     /locus_tag="VFC01_23235"
     CDS             15753..15911
                     /locus_tag="VFC01_23235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82563.1"
                     /translation="MASHPMGPGSAIPPSEWHSSANVYTRAQMMACLRAGVIALVLLG
                     ILALIVLF"
     gene            complement(15975..16397)
                     /gene="sodN"
                     /locus_tag="VFC01_23240"
     CDS             complement(15975..16397)
                     /gene="sodN"
                     /locus_tag="VFC01_23240"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HZN82564.1"
                     /translation="MLQRLFTNATPAHAHCDLYCGVYDPAQAKIEALSCLKSLQKYAD
                     SDDEHFKARAILIKEQRAEEVKHHLMVLWADFFTKEHFEQFPNLHQLFWDGVHGAGDV
                     KKSLDVAVAEKLLKTIDEIADIFWQTEKAKGMGVYPPA"
     gene            16483..16758
                     /locus_tag="VFC01_23245"
     CDS             16483..16758
                     /locus_tag="VFC01_23245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HZN82565.1"
                     /translation="MRPSLRPGDGLLALRGGDPQPGQLRVFPDPTLPSRWLVKRVGDV
                     RGAGRSATFEACSDNPSAPGVVDSRQFGWVPVTGSYRVLWTVRARRG"
     gene            16891..17424
                     /locus_tag="VFC01_23250"
     CDS             16891..17424
                     /locus_tag="VFC01_23250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HZN82566.1"
                     /translation="MEMGSGRAIEIAPFHSRGSLKGFVISGRWPDSTKEWAQLLMVAV
                     RVASLPGLLTTTTIFGAREELPDQPEPGTVGLVVAEGPVVGESAVPPGYFAEHQPPAL
                     LMLHPPSETMPSLPECTGAASGCVLLPGLPYLGLEHRAAWVEAEADGTVTSMVSRVGV
                     DPISHPDTAILAMLLAA"
     gene            17680..18381
                     /locus_tag="VFC01_23255"
     CDS             17680..18381
                     /locus_tag="VFC01_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HZN82567.1"
                     /translation="MLTAALVSLSVVFFAELGDKSQLMTMTYALRHRWWVVLSGVGIA
                     SFMVHGLSVTIGHFLGLTLPERPIAFAAAIAFLFFAVWTWREGRAGGDGREVKLAEPR
                     HVLLAVISSFVLAELGDKTMLATVALASDHNWAGVWIGATAGMVLADGVAIAVGAVLH
                     KRLPERFLHGLASVLFLLFGLWLLLDGALGFRLIALGVTGTVAVSAVTAATVTLVRSR
                     REHSVARPPLESSSQ"
     gene            18827..19225
                     /locus_tag="VFC01_23260"
     CDS             18827..19225
                     /locus_tag="VFC01_23260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN82568.1"
                     /translation="MSKTFAARLNRLFDTVYPPGRGPHTSAEVIAALKAEGVTMSAPY
                     LSQLRSGNRTNPSAATMAALANFFRIKPAYFTDDEYYEKLDKELTWLANMRDEGVRRI
                     AARTVGLSAEAQQDIAQKVDELRRREHLDD"
     gene            complement(19277..20416)
                     /locus_tag="VFC01_23265"
     CDS             complement(19277..20416)
                     /locus_tag="VFC01_23265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82569.1"
                     /translation="MIIWVIAGLLGLATGLRIGWALVNKQSLVSTAMILALGSLGLVA
                     ALNWQPLTLMLDTLLRWNNISMGFSQVALIGCAAGSCVMITTVASERSPATIRKIAIA
                     QYSVAGVIAVVSLVTFFAADQQEEMSPEEYLKRNLGSSDGRVAWLLPLLYVFLALSLV
                     SWAGMRNSNRSRRGRALFVFTLGVVLIVLASAFFLLRAAGNTQLLGVGAAATLLGCAM
                     LVVAAGSLLPSVEDWFGARYELRMVQPLLRELGRRHPDVGIGVRPRGPLVFRVAERMS
                     LISDALFLEATAADATPKRRVDRDDDEVDDVSELESPSVQPREQARTIAQWIYDGRED
                     SKNGASANFPGLGWLRQPTSYSDREWILEIAQQYRQLALSNNESA"
     gene            complement(20413..20904)
                     /locus_tag="VFC01_23270"
     CDS             complement(20413..20904)
                     /locus_tag="VFC01_23270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF955 domain-containing protein"
                     /protein_id="HZN82570.1"
                     /translation="MPASRRVTRAVGAVLDLAPRRGEVSLPRLVDAVSEDRDRAIELK
                     MADLPPGVCGQWRQYADRDVFLIQKGLPAWDRTLAHELGHLVLGHEGIPVVQAARENT
                     EMASSDLIGYMLNQRTGCMGPAGEDAEQEAEDFAALLIYRLGRLPSDRSSIVQVRLGE
                     AFG"
     gene            complement(20966..22054)
                     /locus_tag="VFC01_23275"
     CDS             complement(20966..22054)
                     /locus_tag="VFC01_23275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82571.1"
                     /translation="MGSRVRTAAAACLVASGLLAGGAGASIAFADPQLADGGHGGKHT
                     NDSSDFDPDDKKTTPHPGDKEDQDPADPKPEDQKPEDQKQDDGDGKNGDNNNGQNGVG
                     NPGNGNCGNSGGNGNAHGCGGGNPTDPDDPTPPTPPTKTTTPSTKPEEPPPPEDPGEC
                     EEKNDDHCSPGWPWPWPPGEPPGPGGGGGGGSAPEVPSGRPGLPPPMQLPPELMPPAT
                     EPGAPSVIDVVPGVGAAAVELPIPPLTLPVIVALPAGLGAGGGGAGAPAGPALPAAPR
                     GVNAEPPAGREPLPASVGSNVAVPAASYRVGYSEYLRSAGLPQVAALAVPGVAGMLVL
                     TGAGGLVGYRQAKAGFAVHTSGTARFVN"
     gene            22263..22916
                     /locus_tag="VFC01_23280"
     CDS             22263..22916
                     /locus_tag="VFC01_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6474 family protein"
                     /protein_id="HZN82572.1"
                     /translation="MSLFKRKTRATRRAEARAIKAKAKLEAKLAAKNEARRIKTQHKA
                     ETRALKAQLKAQRDSDRAALRVAETQLKAAREGKLFSPTRIRRVLTVTRLLAPIAVPL
                     FYRAAIAARGFLDERRADRLGVPLSQLGQFSGHGAALSARIAGAEQSLRLVAEKKPKD
                     AETKQFVAAITERLRDLSAAVAAAENMPTSRRRAAHAAIAQQLDGIDADLMARLGVA"
     gene            22929..23435
                     /locus_tag="VFC01_23285"
     CDS             22929..23435
                     /locus_tag="VFC01_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HZN82573.1"
                     /translation="MSRLLAALVLAISFTVSAVAAAGVASAHATRVATDPVENTELTQ
                     SPQKVSAMFSEALQPEFAAMTVVGPDGNLWSTGDPQVDGAVVSVGVRPLGPSGTYTVN
                     YRVTSADGHVVSGSWSFRLAVSGTGTPGPSASAASPASDGIPVWPFFVAAGLIVAGGA
                     VWAVRRQR"
     gene            23432..>23662
                     /locus_tag="VFC01_23290"
     CDS             23432..>23662
                     /locus_tag="VFC01_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopD"
                     /protein_id="HZN82574.1"
                     /translation="MTRRRAVAGAALIVAASVVLACMLAGSQNPPAATLVRAVADGAA
                     VVAIGLAAVPMLDVDRYRGELIRRATGPLTIAS"
BASE COUNT         3861 a         8007 c         7759 g         4035 t
ORIGIN      
        1 acgccgccga tcaacccgac ctagtctcgg cgaccagacg caaaagtgcc caaaattgcc
       61 tgaaataggg cacttttgcg tctgctcgcg ctagagccac cagcgttcga gtacgcgggc
      121 gacgccgtcc tcgacgttgg acgcggtgac ttcgtcggcc gctgccaccg cgtcgggatg
      181 cgcattgccc atcgccaccc caaggccggc ccagtgcagc atcgggacgt cgttgggcat
      241 gtcaccgaac gccaccacgg attcggcggt gatacccaac gggcgggcaa gttcctcgac
      301 accagtggcc ttgctgatgc caagcggcac gatctcaata agtccgttgt tggtggagta
      361 ggtgatatcc ccttccagcc caacatgttt ggccaacgca gcggccatgt cggagctgcg
      421 cgcaccggcc ttgcggatca gcagcttgac cgcgggcgcg ctgagcagat cctcgatgga
      481 cacctcggtg ttgtcggggt tgagccaggc gtgctcgtaa ccgggcgagc tgacgaactg
      541 cggggtggcc gcatcgtgag cgctgttccc gacccgctcg acggccagcc ccgcgccgtc
      601 gatgacgcgg gtggcgacct cggcgagttc gcccagcgca tcggcggaca gcgtgcgggc
      661 cgagataatc cggtccgtgg ccgggtcgta gatcactgcg ccgttggcgc acaccgccat
      721 tggcgcgaaa cccaaggcgt cgaccaccgg ctgcacccag cgcggcgggc gtccggtcgc
      781 gagcacgaac tgcgcgccgg acgccactgc cgcgtgcacg gcgtcgcggg tgcgggcggt
      841 gatcttctca tcgtcgtcga gcagcgtgcc gtccacatcg gaggcgatca ggtcaggggt
      901 caccggtgac tcttcgcttc gcgctcggcg cgcttgcgcg cccgctcggc gagctcggcc
      961 tcttcgagcg ccttggcttc gtccatcgtc ggcgcggaac cgcccagccg gcacggcacc
     1021 cagtacgcgc cctcgggatg cgggtattcc tcctgcaccg tgcgcagaag cgtagtcatc
     1081 ccctcacgca tcgcggcgct cgtctcatcg gtggtctcgg cggcatcgag gggtggtccc
     1141 gctgcgacgg tgactggaat cttgctgcgc cgccccatat ctcgtgggtg atccttggtc
     1201 catcgccggt gtgcacccca cacgatcagc gggacgatcg gtacctcggc gtctcgcgcc
     1261 atccgggccg cgccgctctt gaactccttg agctcgaagc tgcggctgat ggtggcttcg
     1321 ggatagacac caacgatctc gcccgcccgt agtcgctcga ccgccaccgc gtacgcacct
     1381 cccccggagg tgcggtccac cggaatggtg ccggaatgct tgatcagaaa gttgacgatc
     1441 ttgacgtcct gcatttcggt cttgatcatg aaccgcattc gacggtgccg ctgataggcc
     1501 gcgagcgccg cgggccacca gtcgacgtaa ctcgtgtgat tgatcgcgat taccgcgccg
     1561 ccgcgatccg ggatgttttc cagtccctgg taggtgatct tggtcccgat ggccttggtc
     1621 gccaggctga cggcaatctc gagcgtgcgg aagaccggct ccatacccgc taccccggcg
     1681 ccccggtggg gtcggtccgt tgctcgcggc gcgcggcctt ctcggccgcc tcctcggcgt
     1741 ccatccgatt ggcctcggcc agggtcggcg ctcccccacc cagccggtgt ggcacccagt
     1801 actcgccggg tggatacggg ccgtattcgt cctgcacgcg ctcgagcagg tgctgcatcc
     1861 gcgagtgcag cagcgcggtg agctccgtcg gcggcagggt cggcatgatc ggctcaccca
     1921 ccgccaccga gatcggcacc ttggggcgcc acatcttctt gggatgcccc ttggtccaga
     1981 tgcgttgggc gccccacacg atgtgcggca cgatcggcac gtctgcggcg accgccatcc
     2041 gtgccgcgcc ggacttgaac tccttgatct cgaagctgcg gctgatcgtg gcctccgggt
     2101 agacgccgac cagctcgcct tccttgagcg cttgacaggc gtggtcgaac gaggcggcac
     2161 cgctgtcacg gtccacctcg atgtgccgca ggctgcgcat aattggcccg gtgaccttgt
     2221 tgtcgaagac ctctttcttc gccatgaacc gcaccttgcg gccgaggtgc tgcttgtagg
     2281 cgggtagtcc cgcgaaggtg aagtcgaagt agctggtgtg gttgatcgcg acgacggcac
     2341 cgcccgtacg gggcagattc tcaacgccgg tcacggtgaa cttcaacccc tgcgcccgcc
     2401 ataccaggcg ggcgagctgg atgactgtgc cgtataccgg ttccacatca ggcagcctag
     2461 tcgggtgacc gctcccagcg aacccgtgcc agttccggag accgtcgagc gcatcgcggc
     2521 cggacggacg gtcgtcgcgg tgtggatcaa cgagttgggc ggcgtcacct acgcagtcga
     2581 tggtggctgc gagtacgtca aggtcgcgcc atcggagtgg gcgcatcatt tggtggctga
     2641 ggtggctcgg ctgcgctggg cggcggctta tgtccgggta ccccgcgtga tcgggtccgg
     2701 cgacgggtgg cttcataccg cggggctgcc aggccggtcg gctgtcgacc cgcgctgggt
     2761 ctcggatccg cggaccgcgg cccgcgcgat aggcgccggg ctgcggatgc tgcacgacac
     2821 gctgccggtc gacgactgcc cgttcggccc accgccgtgg gtgaccgact ccctgccggc
     2881 cgatcggctg gtgatctgcc acggcgatgc gtgcgcgccg aacaccttga tcgatgacga
     2941 cggccgctgc tgtgggcacg tcgacgtcgg agacctcggc gtcgccgatc ggtgggccga
     3001 cctggcggtg gccacgctgt cgctgtcgtg gaactatggc ggctcctggg aaggcgacct
     3061 gttcgatgct tatggcgtcg agccagacac gacgcgcatc gcgtactacc tagggctctg
     3121 gaacgccggt gatatctcgt cccgctaagc tcagaccggc acaaccgtcg ggaagaaagg
     3181 gtgtttgtgc aggtcacgag cgtgggacac gccggtttcc ggatcgacac caaggccgga
     3241 agcatcctgt gcgacccgtg ggtcaatccg gcgtacttcg cgtcgtggtt cccgttccct
     3301 gataacagcc agctggactg ggccgcgctc ggcgacgtcg actacctgta cgtctcgcac
     3361 ctgcaccgcg accacttcga cccgaagaac ctgcgcgacc acgtcaacaa ggacgcggtc
     3421 gtgctgctgc cggactaccc ggtgcccgac ctgcagcgcg aactggagaa ggtgggcttc
     3481 acgcggttct tcgagacgac ggattcggtc aagcaccgcg tcagcggacc caagggcgac
     3541 ctcgatgtga tgatcatcgc gctgcgcgcc cccgccgacg gcccgatcgg tgactccggt
     3601 ctggtggtcg atgacggaga aacggtgtgc ttcaacatga atgacgcgcg ccccgttgat
     3661 ctcgacgtgc tgcacgcgga cttcggccat gtcgacgtgc acatgctgca gtactcgggc
     3721 gcgatctggt acccgatggt ctacgacatg cccgcgcggg cgaaggaggc gttcggcacc
     3781 cagaagcgtc agcgccagat ggaccgctgc cgtcagtaca tcgcgcaggt cggtgcgacg
     3841 tgggtggtgc cgtcggccgg gccgccgtgc ttcctcgatc ccgaactgcg tgacctcaac
     3901 gacgaccacg gcgaccccgc caacatcttc cccgaccagg cggtcttcct cgaccagatg
     3961 cgaacgcacg gccacgacgg cggcctgctg atgattcctg ggtcgacggc ggatttcact
     4021 gggccgcaat tgaactcgct gacccacccg atgccggatc cagagtcaat cctcaccacg
     4081 ggcaaagccg cctacatcgc cgactacgcc gagcggatgg caccggtgct ggccgcggag
     4141 aaggccagct gggcacccgc ttcgggtgaa cccttgctgg agccactgcg gatcgtgctc
     4201 gagccgatca tgctgcagag cgatcagatc tgcgacggca ttggctatcc ggtcgagctg
     4261 cgcctcggcg cggagactgt ggtcctggac ttcccgaaac gcgtagtgcg cgagccgatt
     4321 ccggacgaga agttccgcta cgggtttgca atcgcgccgg agctggtgcg cacggtgctg
     4381 cgggacaacg agccggactg ggtgaacacc atcttcttgt cgacgcgctt ttcggcgtgg
     4441 cgggtcggtg gctacaacga gtacctgtac acgttcttca agtgcctgac cgacgagcgg
     4501 atcgcctacg ccgacggctg gttcgccgag gcgcatgacg acaccgcgtc aatcatgttg
     4561 gacggctggg agattcagcg tcgctgcccg catctgaagg ccgacctgtc gaagttcggc
     4621 gtggtggagg gcagcacgtt gacctgcaat ctgcacggct ggcagtggaa cctggataac
     4681 ggccgctgct tgacgacgaa gggccacgag ctgcggtcgg agcgaaaatg acggccccgc
     4741 gccagtacta cgacgacggc ttggtccagc tggatcgtga ggcgatcacg ttgcggcgct
     4801 accactttcc gtcgggtaca tcgaagatca tcccgctgcg gtcgatccgg ggctataagg
     4861 ctgaatccct ggggctgatc acggagcggt tccgcatctg gggcagcacg gacccacgcc
     4921 gttggatgcc gttggacgtc tggcggccga tcaagtccac actcgtgacg ctcgacgtgc
     4981 ccggtgtgcg acccagtccc gcgttcaccc cgcagcggcc caaggaattt ctcgcgaggc
     5041 tcgacgagct gctgaacgac tagccgaatt cgagtagtgc actacgcacg ccgcactcgc
     5101 tgtcatcgtc gtcgatatgg ctagcgaaca gagcaaacag agcaagaagg gcttcgccga
     5161 ggaattcgaa gaattgaagg cgaggcacgg tgcacagtac gcggatctga gattggtccg
     5221 cttcacggat aagacggtca ggtcggcttt cgggcgttcg tgccatattt ctgccgtgag
     5281 aacgacgacg gcgacatcgt ctgtgaggaa aagcgtccgg tctgacgact agcgtttcgg
     5341 cttcagcacg tcggtcccga cgtacggcac cagggcatcg gggacccgga cgctgccgtc
     5401 gggctgctga tggttctcca ggatcgccac cagccagcgc gtcgtggcga gcgttccgtt
     5461 gagcgtcgcc gcggtctgcg gcttgccgtt ctcgtcgcgg tagcgcacag acagccgccg
     5521 ggcctggaac gtggtgcagt tcgacgtcga cgtcagctca cgataggtct gctgcgtcgg
     5581 gacccacgcc tcgcagtcga acttccgggc cgccgaggac cccaaatcgc ctgcggcgat
     5641 atcgatcacg cggtacggca cctcgatgtg cgccaacatc tggcgctgcc aatccagcag
     5701 ccgttcgtgc tcggcctcag cgtccgccgg cttgcagtag acgaagccct cgaccttgtc
     5761 gaactggtgc acgcggatga tgccgcgggt gtccttgccg tggctgcctg cctcgcggcg
     5821 gaagcacgac gaccagccgg cgtagcgcag cggtccgtcg gacaagtcga ggatttcgtc
     5881 ggcgtggtat ccggccaacg gaacctcgga ggtaccgacg aggtagaggt cgtcggcctc
     5941 gatccggtac acctcctcgg cgtgtgcgcc gaggaaaccg gtgcccgaca tcacctccgg
     6001 ccgcaccagc accggcggga tgacaagggt gaaaccgttc tcggtggcca gccgcaccgc
     6061 gagctgcagg aggccaagct gcagcagcgc accggcaccg gtgaggaagt agaaccgcga
     6121 acccgacacc ttggcgccgc gttcgaggtc gatcaggccc agcgactcgc cgagctcgac
     6181 gtgatccttc gcgttctcga tggcccgtgg ttcgccgacc gtttcgcgca ccacgaagtc
     6241 gtcctcgccg ccggccggca cgccgtcgat gatcacgttg gagatcgtca tatgcgctgc
     6301 ggtaaaagct ttttcggctt cggcctgttc ggcctcggag gccttgactt tgtcggcgaa
     6361 cgccttggcc tgttcgagga gcgccggtcg ctcatcgggc gatgccttgc ctacctgctt
     6421 gctaacggcc ttctgctcgg cgcgcagatt gtcggcggcc gacaccgcgg accggcgtgc
     6481 ggcgtcggct tcgaggagcg catcgacgag tccggggtct tcaccacggg cccgctgaga
     6541 cgcgcgcacc aagtccgggt tctcgcgcag caccttgagg tcgatcacgg ccgcaacact
     6601 actttttggc gcccggcctc ggcgggggga cccacgtcag gcacaacctc gtaacgttac
     6661 atctgcatca cttgtcacag cgcctgacac gcctgtcaca atggagcgga tgttggacgc
     6721 acccgagcgc gaggatttac tcgctgactc gcacaagagc gacgacggac gccgcgtggc
     6781 gtggtggcag agtttccacg ctacgtccac ccgccgcgcg ctgttgctga ccgcgctcgg
     6841 tggtctgctc atcgccgggc tcatcaccgc gctgccgagc acggacggct ccaacggcct
     6901 gaccgccaac tcgatctccc tcggtccccg cggcaacgac acgttcaacc acgccaacag
     6961 cggcgactgc ctgaactggc ctgatcgcac cccggacgcg ggcgagatcg tcgattgcac
     7021 gggcgatcac cgtttcgaag tcgccgagtc ggtcgacatg cgtaccttcc cgggcagcga
     7081 gtacggcccg gacgcggcac cgccgtcgcc cgcccggatc cagcagatca gccaggagca
     7141 gtgctcggca gccgtcaagc gctacctcgg cgcccggttc gacccgaaca gccgcttcac
     7201 cgtcagcatg ttgtggtcgg gtgagaaggc gtggcggcag tctggtgagc gacgcatgct
     7261 gtgcggcttg cagctgccgg ggccgaacaa ccagcagctg gcgttcaagg gcaaggtcgc
     7321 cgacgtcgac caatcgaagg tgtggcccgc gggcacctgc ctgggcatcg acccgagcac
     7381 caaccagcca acagacatcc cggtcgactg cgccgccccc cacgccatgg aggtcacggg
     7441 tgcggtcaat cttgcggaga agtttccgca ggcactgccg ccggaacccg agcaggacgg
     7501 cttcatcaaa gacgcctgta cgcggatgac ggacgcctat ctggcaccga tccagctgcg
     7561 cagcaccacg ctcacgctga tctacagcac catctcgttg ccgagctggt cggccggcag
     7621 ccatcaggtg tcgtgcagca tcggcgcgac gcttggcaac ggcggctggt cgacgctgct
     7681 caacagcgcc aagggtccgc tgcagatcaa cggccagcca ccgatcccgc cgcccgacat
     7741 ccccgaggag cggctgaacc tgccgccgat cccggcgatg gagccccaga tgccggatga
     7801 cgactcctcg tactcctcct cctcgcaggc gaccaacagc gacaccaccg accacggcag
     7861 ccagcagacg gggacccagc acatgcccgg gcagcagtcg acgggccaga ccgggacgtc
     7921 gacggtcccc gcgccgcccc cgccgggcaa cacgttcctc aacgggccac cgccgcccgg
     7981 cgcgcccctg gatgcgcctc cgccaccgga gggcgcccca ccgccgccgc ccggcgctcc
     8041 tcccggtccg gctcccgctc cgcctccggc tgaggtcgcg cccgcgcccg gcccaccgcc
     8101 tccgccgggc gatccgctac tgctgccacc gccgccgggg cagtagccgc gtgaccgtgc
     8161 ggatgagccc gcaacggttt gacgagctgg tctccgacgc actcgacctg attccaccgg
     8221 agctcgccgc cgcgctcgac aacgtcgtgg tgctggtcga gggccgcaac cccgacgaac
     8281 ccgagatcct cgggctgtac cagggggtgg cgttgaccga acgggattcg tggtacgcgg
     8341 gctcgctgcc cgacaccatc accatttacc gcgacgcgtt gctcgaggtc tgcgaggacg
     8401 aggctgaggt cgtcgacgag gtcgcgatca cggtgatcca cgagatcgcg caccacttcg
     8461 gcatcgacga cgaccggctt catgaactcg gctggggcta ggcgcggcaa ccacgcttcg
     8521 tccgcgtccg gtgctttcat gggccctatg agcagtgaat gccgtgactg cctggcaggg
     8581 ctcgagcact gccacggcac cgtcattcac cacgcctggt atcgcacgga atgcaccgag
     8641 gacggctgcg tcacccccga ggtggtgcac ctgttcagca tcgactgtga ggcgatcggc
     8701 tgcacctgcg acgccgccgt gtcagcccat cgggtcggct gaggacagcg cgtcctctac
     8761 ggggtgcaca tcgggctcgg ggtagaacgg cggcgtcagc gtcgaccact gcacgcagct
     8821 ccaccgtccg tcggtaatcg gtgacagcac aacggattcg gcgttgggca ggtgatggtc
     8881 gagggcgaag ctggagtcga cgccggccag cacggccgac acgagccgga tcgccgcgcc
     8941 gtggctgacc accacgatgt cgccatgcca cgcgtcgtca tcgaggtagc gcatccgcaa
     9001 ctgcgtgagc accggcacat agcggtcgag cacctcgttg ccggtctccc cgccgggcat
     9061 cggcacgtcc aggtcgccct cgtgccagcg ttggtagacg gtttcgaact cggcgatcgc
     9121 atcgtcgtcg ttgcgatcct cgagatcgcc cacttggacc tcgtggatgc cctcgaattc
     9181 gtgcgtgtcc aggccgagtg cgccgctgat ctccgcagcc gtttgctcgg ctcgcaccgc
     9241 caccgagtga gcgatcatcg ccgccggatg catcagctcg cgggcgaagg cgcgggcctg
     9301 gtcgcggcca aggtcggtca acgccgcccc tggcggcctg gtgtcgaggc gacgttcgac
     9361 gttgccgtgc gactggccgt gccggaccaa gaccagccgg ccactcacgg cttgccctcc
     9421 cgcacccgcg tcagccatcg ggacgcctca tccagatgcg gcggcgcggc gccggcggcc
     9481 gaccccgtcg gccaggaacc caggtatctc acgtctgcac aacgacggtg caacgccttg
     9541 agtgcctcag cgaccgcctc gtcgtcgatg tgcccgacac agtcgaggaa gaagaaatag
     9601 gtgcccaact cctttcgggt gggccgggat tcgattcggg tgagatcgat atcgcggatc
     9661 gcgaactcgg tcagtgccga cacgagcgct ccgggaacat tgtcgaggcg cagcaccacc
     9721 gaggtgcggt cggcgccggt gcggctcggc gggggcgccg gctgtccggc caatacgaac
     9781 cgggtgcggg cattgggctc gtcgaccacg tcggtggcca gcgccgtgag accgtagcgt
     9841 ttggctgcca gagccgtggt tacggcggcg tcggcccggc cctccgccac atcgacggct
     9901 gccgcggcgt tggagtccgc cgggtggaac tccgcttccc gcagattctc cgcagcccat
     9961 ttcgctacct gcgcacgggc caccggatac gcagcgatcg acgcaggccg cgtgacacct
    10021 gcccgcaccg cgatcgtgaa cccaatgtcg agcgtgaact cggcaaagac ctgcattggg
    10081 gaaccggtgg ccaggttgtc cagcgtcggg agcacggagc cgtcgatcga gttctcgatg
    10141 ggcacgcacg cgtaagtggc atcacccgcg cgaaccattt ccagcgccgc cgaggggctg
    10201 ctggccggga acggttcgaa gtcggacaaa ggcaccaggt tcgcggcgac cattcctcgc
    10261 aacgccgcct cggtgaacgt tccttcgggt ccgaggtagg cgatgtgggg cacgtttcaa
    10321 ccctatcggg tgtggccccg accggccctg atgtcgctgg gtaggccttg cggctcgctc
    10381 gcccttctag ttaagtaagg cttacctcac tagcggaagg cggcgagatg acagcagcgg
    10441 caccgacgac ggccgagcgg atacgcagcg cgtgcgtgcg ggccggcggc gccatgctcg
    10501 cggtcgaggg cgccgagccc acctccaccc ccgtgcatca cctcctcgac gacggatcgt
    10561 tcgcgctgac ggtccctcgc gacggcatgc tggccggaat gacggtggcc gcgggcgccg
    10621 aaggggtcca ggcggtgttg gagatgaccg actacgcccc gctgccgctg cgcgagccgg
    10681 tgcgctcgct ggtctggatc cgcgggcgcc tgcacggcat ccccgacagc gaagttgctg
    10741 cgctgctcga cctcatcgcc gaggaggacc ccaatcccgc tctgctccaa gtgaattcag
    10801 agtcgacgtt ggtgagactt gagatcgagt cggtggtggt cgcggactcc accggtgcgg
    10861 aatcggtcgg agtgagcgcc ctgctcggag ccaggcccga cccgttctgc gcgatggaat
    10921 cgtgctggct caagcacatg gagtccgcac accgtgaggt cgtcgaccgg ctggccagcc
    10981 ggttgccgat gccgctgcgc cgtggccggg tgcggccgct gggcctggac cgttacggag
    11041 tgcagctgcg tgtggagggc gacgacggcg atcacgatgt gcggctgccg ttcgccaaac
    11101 cggtcgacga cgtgaccggc ctcagccagg cgatccgcat attgatgggc tgcccgttcc
    11161 tcaatgggct gcgcgcccgc cgactctagg gctcggctgc cgctaccgta tctccggtga
    11221 cagggccgcc cgacgagctg accgatccgc aacggcgtgt catccgcatc gagatcgtcg
    11281 tcgtcctggc cgtgtcgttc gggctgtcgg cctacaccgc gtttctcaac ctgatcgagg
    11341 cggtgctgct cggcctcgcc ggccagcgcg tggcgctcaa tccccgtcgc tcaccgttcg
    11401 acctcatcga tctcggcctg aaccttgccg tcgccttcca gctcgtcgca tggggcgcgc
    11461 tcggcgtata cctgttgtgg cgcagcggct tcggtcccgc gcagatcgga ctcggcaggg
    11521 tgcgctggcg gccggacgtg ctgggcggcc tcggcctcgc ggcgctgatt ggcctaccag
    11581 gactgggctt gtacgtggcc gcaagggcgc tgggattgag cgcgcacgtc gagcctgtcg
    11641 agctgtacga cacctggtgg cggataccgg tgctgctctt caccgcgttc gccaacggct
    11701 gggccgaaga agtcatcgtg gtcgcgtttt tgctgacgcg gctgcgccag ctgcgggtca
    11761 acccggtgac cgccctgctg gcctcgagtc tgttgcgcgg cgcgtatcac ctctaccaag
    11821 gcttcagcgc ggggctcggc aatgtcgtga tgggtctggt gttcgcctac gtgtggcggc
    11881 gcaccggtcg gctgtggccg ttgatcatcg cgcacgggat catcgactcg gtcgcctacg
    11941 tcggctacgc gctggcgtcg ggccacctcg gctggctgtc gtagattcat tgggtgagcg
    12001 acaaccggcc accgccccgg ggtcacgacc ccagggtgcg tcgggaaccg tcgcaagtga
    12061 tccggcgtga cccgaactac cggccgccgc ctggctggcc gccgaacccg ccgactcccg
    12121 cgcggcgacc gccaccccct cccccgccac cgcgcaggcc gccacccgtt ccaccccgac
    12181 aacagcacca gccgccgccc ccgccgaaag cgaaaaagcc tcgccgcaaa cggcattggg
    12241 gccgcatcgt gctggcattc ctattcgtgt tcgtcgtcgc ggtggtggcc ggcggagtct
    12301 ggatggattc gtcgttgcac cgcatccccg cactggccga ctacccggaa cggccggcga
    12361 ccggcacggg caccacatgg ctactggtcg gctcggacag ccgccaaggc ctcacgccgg
    12421 agcagcaggc ggagctggcc accggcggcg atatcggcaa cggccgcacc gacaccatcc
    12481 tgctggtcca cgtacccgcc atcgggtcga gcacgcccac cacgatggtt tccatcccgc
    12541 gggactccta cttgccgatt cccggctacg gcgaggacaa gatcaatgcc gcgttctccg
    12601 agggcggtgc cccgctgttg gcacagaccg tcgagcaggc caccgggcta cggctcgacc
    12661 actacgccga gatcggattc gacggtttcg cggtcatggt cgacgccgtc ggcggcgtga
    12721 ccatgtgccc gacggagccg atcagcgatc cgctcgcagg catcgacctt cccgcaggct
    12781 gtcaaaaact cgacgggcgc agcgcgctcg gctacgtccg cacccgcgcc acgccgcggg
    12841 cggacctcga tcggatgatc aaccagcgtc agttcatgtc ggcgttgctg catcgcgccg
    12901 ccagtccagc ggtgctgctc aatccactgc gctggtatcc gatggcgcac gcggccagcg
    12961 gcgcgttgac cgtcaacgag ggtgatcaca tctgggatct ggcgcgactg gcgtgggcgt
    13021 tgcggggcga cgtcaccacc acgaccgtgc caatcgggga attcaccggc ggcgactccg
    13081 gctccgtcgt ggtgtggaac agtgatgtcg cgggccagct gttctccgcg cttgcatcgg
    13141 attcgcctgt gccgcaagag gttctggacg ccgccgacaa cggctcctag tcgcggcccg
    13201 cgccagtcag cccgtcctgc agccatgcct tggtgcggcc ggggtgattc gtcgccaccc
    13261 acgccacgcc gacgtcgcgg cagtatcgga cgtcctcgta atggtcgacg gtccagcagt
    13321 acagcgccct gccctgcgcc gctgcccgat ccaccaactc ggggtgctca cgcagggtcg
    13381 cgatggacgg cccgaccgcg gtcgcgccga cggtggtggc ggcactgccg ccgagatagc
    13441 gggaggtctc cccgagcagc acggtaggca gcatcggggc cgcgcgccgg atccgccaca
    13501 ccgcggccgc cgagaacgac atcaccaccg cgcgggacaa gtcagccgac gcgggcgatg
    13561 cgatgccata ccggtgcagc aaggccagca ccttgctctc gaccagtgcg ccatagcgga
    13621 cggggtgctt tgtctcgatg aacatcttga cgggccggtt ccagtccagc acgagggaca
    13681 ccagatcatc gagtgtgagc agaccggtgt caccatggct gccgtcgagt cgccagctgg
    13741 gatgccacgc cccgaagtcg agttcgcgca actgcgccag tgtcatctcg ctgaccagcc
    13801 cggtgccgtt cgaggtccgg tccactcggc ggtcgtgcac acacaccaga tgcccatcgc
    13861 gggtcagccg aacatcgcat tcgacgccat cggcgccttc cttgagggcc agctcatagg
    13921 ccgccgaggt gtgctctgga cgatcggccg atgcgcccct gtgcgccacc acgaacggat
    13981 ggcggccgag cgtgccgtcg tccgagttca tacggctatg ctgccgggtt ttgcccgcca
    14041 gactcaaccg gagccgcgcg ctcgggctcc gattcgggtt gctcctcggc cgttcgggcg
    14101 tcgtctttct ggtcctcacg cacgatcacc cagcgtttgg ccgggcgctc gacgggctgc
    14161 cgctcgaagc cgaagaacac cttcatcacc aacagcagcg ccgccagcgc catcaggtag
    14221 gcgacgatcg tcgtcacagt gttgtctgcg atgccctgcg gatcttgggt aaagctggtt
    14281 gcgatcgaga accccgacac cgcagtgctg aggatccaga caacccacca caccgtgatc
    14341 ggccggccca gccatttcag ccgctcctcg acgtcggcca actcgatgac aaagaccggc
    14401 gcccaaaaca gattcaccag cggcacaagg caacccgcgc gcagcgccca cacagggcgt
    14461 ggatcggggc ggcggtggtg ggcaaaggcg gcggcgcgcc gcgcgatcag ccagttcgtc
    14521 agcaccaccg cggtggcgac gatcatgaac accgcgacga cgctgacgag cacacccaac
    14581 caggtggctg tgaatgccac caccttgttc aacaaaatcg atcggttgac gatcaacagc
    14641 gcgtagcgca gcaagtgcac gagagccgca acaccgaata ccaccatcgt tgcgatgagg
    14701 gtggcacgca cgcccgtaag ggaggggcct gtgcgcagcc cgaccgtctg ctgttccggc
    14761 gcctcgaact ggtccaccag gccccacctg gggatgacgg cgtagcgagg ggtggggccg
    14821 agggcacccc tgcggcggcg aggtggcggg ggcgcgccgg gtcgcaccgc gatccagcga
    14881 tatccgggcg gcagcgccgg ccggcgtccc cctcctgcag gcggcgagcc caagccgcca
    14941 ggtcccgccg gtgacgctgc cgggtgggcg ctccactccg ctccgggcgc gggacccgac
    15001 ggcgcaagca gcgcgccgtg gcatcgcgga caccacaccc gtcggcggtc gcgcacgttc
    15061 caccgcgttc cgcactggga acacacctga atcatttcgc tcctcgcgtg cgcgggatca
    15121 cccgaaccag actagcggcg gcccatgaga tgccgccgta tccccatcgc caactgtcag
    15181 tgcttgcgcg accgcgacct cgccaatgcc gttcgtgcgg acacagcgca tcatcggcgc
    15241 ttcaatgcag tgtctccaga gggccggtaa acgaagcatc agcaattccg gcctatccac
    15301 aatttccaca gcttcatcca cagcccctca acggcccgaa ccccgtttga accggtgaat
    15361 ctgtgtgctt gctgggaatt actgtggata accttgtgcc caaactggcc gccaatcaac
    15421 aggcccgggg ccgccgagcg aaaagagttg ccacgctaag ttcccgcccg ggcatcccgg
    15481 tgccgcggac cagttgtagt tcggttggat gacagataac ggcacgtgtt ggacggctgc
    15541 atcgcgcgtc actgagtttg ctgggccgtt cgcggccccg catctgtgaa gctccaaccg
    15601 cggatttatc cacagggcac aaccggtggt tccacgaccc cggtgtcgat tttccactcg
    15661 ccacaagagg ggttatgatc ggcgtcacac gagcagtttg tgacagatct cactggggga
    15721 gggccacaga tcgtgcaggt caggagggtt tgatggcgtc acatccgatg ggtccgggat
    15781 ccgctatacc gccgtccgaa tggcattcct ccgcgaacgt gtacacgcgc gcccaaatga
    15841 tggcgtgtct acgcgccggc gtcatcgcgc tggtgctgtt gggcatcctc gccctgatcg
    15901 tcttgttctg accaaataca aaagagccgc cgggcaaggg ccggcggctc tgtgcgtgga
    15961 cgacttgagc ggtctcaggc gggcggatag acgcccatgc ccttggcctt ctcggtctgc
    16021 cagaaaatgt cggcgatttc gtcgatcgtc ttcagcagct tctcggcgac ggcgacgtcg
    16081 agcgatttct tgacgtcacc cgccccgtgc acgccatccc agaacagctg gtgcagattg
    16141 gggaactgct cgaagtgctc cttggtgaag aagtccgccc acagcaccat caggtgatgc
    16201 ttgacctcct cggcacgctg ttccttgatg agaatcgcgc gagccttgaa gtgttcatcg
    16261 tctgagtcgg cgtacttctg aaggcttttc aggcacgaca gcgcctcgat cttggcctgg
    16321 gcggggtcat agacaccgca gtacaggtcg cagtgggcat gggcaggggt ggcgttggtg
    16381 aaaagtcgtt gcagcatggc tttaacctac ccttcgaggg tgggccgcaa ccatccgcag
    16441 cgacgctggc cactgcgccg cttcgccgtg gtggaggact cgatgcgacc ttccctgcgc
    16501 cccggtgacg gcctgctggc gctgcgtggt ggcgaccctc agcctggcca gttacgggtc
    16561 ttccccgatc cgacgttgcc ttcgcgctgg ctcgtcaagc gcgtcggcga cgtgcgcggc
    16621 gccgggcgca gtgcgacatt cgaagcctgc tcggacaatc cgtccgcgcc cggtgtcgtc
    16681 gactcgcggc aattcgggtg ggtgcctgtg accggctcct accgcgtgtt gtggacggtc
    16741 cgggcacgcc gcggctgacg ccgtggccgt cgaaagttgg tcattagcgg ccgtgtccgc
    16801 ttgctaccag gtttgacctg ccgcattctg gtcaaatcga cccataggcg tgtcgcggat
    16861 gtatccgaac accgatggcg cggaggcgag atggagatgg ggtctggtcg ggcgatcgag
    16921 atcgccccgt ttcattcacg cggatcactc aagggcttcg tgatatccgg tcgttggcct
    16981 gactcgacca aggagtgggc acagctgctg atggtcgccg ttcgcgtcgc ttcccttcct
    17041 gggttgctaa ccacgacaac gattttcggt gcccgcgaag aactccccga ccagccggag
    17101 ccaggaacgg tcggattggt ggtcgccgag ggcccggtcg ttggcgaatc agcagtgccg
    17161 ccagggtatt tcgcagagca ccagccgccg gcgctgctga tgctgcaccc gccctcggag
    17221 accatgccgt cacttccgga atgtacgggc gccgcgtcgg gatgtgtgct gctgcccggg
    17281 cttccgtatc tgggtttgga gcatcgcgcc gcctgggtgg aggcggaagc agacggcacg
    17341 gtgacgtcga tggtgagccg tgtcggggtc gatccgatca gccatcccga cacagctatc
    17401 ttggccatgc tgcttgcggc ataagggttt tcgaacgatt tgattcgttg tcctgaacgc
    17461 tcgattcggg cacccgaact cgtgccgccg agtggcgctg ccgcgaaaac gccgctatcg
    17521 tcgatgaagt cagcgaaggg gagtagcccc caatcgtcga gtcgacatac tggcgtagag
    17581 cccggccggc gaaccgcagt gcggtgggcg agaccttcga ccgaagtctg acgggtcgcg
    17641 agcaaagacc cgtcggcgac gtgtcgaaag gtcgttccca tgctcaccgc cgcactggtc
    17701 agtctctctg tggtgttctt cgccgaactc ggcgacaaat cgcagctgat gaccatgacc
    17761 tacgccctac gccaccggtg gtgggtggtg ctgtctggcg tcggcatcgc ttcgttcatg
    17821 gtgcacgggc tgtccgtgac gatcggccac ttccttggcc tcacgctgcc ggagcgccct
    17881 atcgccttcg ccgcggcgat cgccttcctg ttcttcgcgg tgtggacatg gcgggagggc
    17941 cgggcaggcg gcgatggccg ggaagtcaag ctcgcagagc cgcgacacgt gctactagcc
    18001 gtcatctcct cgttcgtcct cgccgaactc ggcgacaaga cgatgctcgc cactgtcgcc
    18061 ttggccagtg accacaactg ggccggggtc tggatcggtg cgacagccgg catggtcctc
    18121 gccgacggag tggccatcgc ggtcggcgcc gtcctgcaca agcggttgcc ggagcgcttt
    18181 ctgcacggtc tcgcgagtgt gctgttcctg ctcttcggac tgtggctgct ccttgacggc
    18241 gcccttggct ttcgtttgat cgcgctcggc gtaaccggga ctgtggccgt gtccgcggta
    18301 accgcggcca ccgtgacact cgtccgcagc cgtcgcgagc attcggtcgc ccggccgccg
    18361 ctcgaatcgt cgtcgcagta gcgcagatca cccactggct actgtcggtc aaatgactga
    18421 ccaggttcca acgatgatct cgctatcgcg cgtcacgtgc cgaggaacgg accgacaccc
    18481 gtcaacaccg accgcttgcc tacgcaacta gcaaacatgg gtttccgctt tgcggcgcgt
    18541 gagagcagtt gccagtgggg aacatcggtg ttcctgtgac gaacagtgac aaagttctag
    18601 gaaagtcatc gccgcgcaac cgttattatt tgcttcgccg caccgtagga tgtcggtgcc
    18661 gggcctaccg acaacgccgt taagacctgt agcggccaca caaaactaaa aggcgggtta
    18721 acttttccgc ggtttgcact tggttgttgg cacaactctc gctacgatga tcagcaaata
    18781 cgccgcccta cgaaacccgc tcgtccagca tgtggaggtc aaatcgatga gcaagacgtt
    18841 cgccgcccgt ctaaaccgcc tgttcgacac ggtttacccg ccaggtcgcg gtccgcacac
    18901 gtcggctgaa gtcatcgccg cgctcaaggc agaaggcgtg acgatgtcgg ctccgtacct
    18961 gtctcagctc cgctcaggca accgcaccaa cccctcggca gcgaccatgg ccgcccttgc
    19021 caactttttc cgtatcaagc ccgcgtactt caccgacgac gagtactacg agaagcttga
    19081 caaggaattg acgtggcttg ccaatatgcg cgacgagggt gttcgccgta ttgcggcgcg
    19141 caccgtcggg ttgtcggcag aggcccagca ggacattgcc cagaaggtcg atgagcttcg
    19201 tcgccgggag cacctggacg attagcccgc cgttcgccgt tacgcggcgt gtggcgaatt
    19261 tgggaaattc ggtcgcttag gcgctctcat tgttcgagag cgccagctgg cgatactgct
    19321 gtgcgatctc gaggatccat tcgcgatccg agtacgacgt gggctggcgc aaccagccaa
    19381 gtccggggaa gttagcgctc gcgccattct tcgagtcttc tcgcccgtcg tagatccact
    19441 gcgcaatcgt ccgtgcctgc tcgcgcggct gcaccgacgg tgattcgagt tcggacacat
    19501 cgtccacctc gtcgtcgtcg cggtcgaccc gtcgcttcgg cgtggcatcc gccgcggtgg
    19561 cctctaggaa cagcgcgtcg gagatcagcg acatccgctc ggcgacccgg aacaccagtg
    19621 ggccccgggg gcggaccccg atgccgacat ccggatggcg tcgaccgagc tcccggagca
    19681 gcggctggac cattcgcagc tcgtatctgg cgccgaacca gtcttcgacg ctgggcaaca
    19741 aggagcctgc ggcgacaacc aacatggcgc agcccagcag agtggccgcc gccccgaccc
    19801 cgaggagctg ggtgttaccc gcggcccgca gtaggaagaa cgcggacgcc agcacgatca
    19861 acacgacgcc gagcgtgaag acgaacagcg cgcgaccacg ccggctgcgg ttggagttac
    19921 gcatgccggc ccacgacacc aggctcagcg ccaagaagac gtagagcagc ggcagcaacc
    19981 acgccaccct cccgtcgcta gaacccaggt tgcgcttgag gtactcctct ggcgacatct
    20041 cctcttgctg gtcggcggcg aaaaaggtca ccaagctcac gaccgcgatg acaccagcaa
    20101 cgctgtattg cgcgatagca attttgcgaa tcgtggccgg agaccgctcc gacgccacgg
    20161 tcgtgatcat cacgcagctg cctgccgcgc agccgatcaa cgcgacctgg gagaacccca
    20221 tcgaaatgtt gttccagcgc agcagggtgt cgagcatcag cgtcaacggc tgccagttga
    20281 gcgcagccac cagcccgaga ctgccgagcg cgaggatcat cgcggtgctc accagcgact
    20341 gcttgttgac cagggcccat ccgatgcgga ggccggtcgc caatccgagc agaccagcga
    20401 tcacccagat gatcaaccaa acgcctctcc gagtcggacc tgaacgatgg acgagcgatc
    20461 cgacggtagt cgtccgagac ggtaaatgag cagcgccgcg aagtcctcgg cctcctgctc
    20521 ggcgtcctca cccgcgggac ccatgcaccc cgtccgctgg ttgagcatgt agccgatcag
    20581 gtcagagctg gccatctcgg tgttttcgcg ggccgcctga acgacgggga tgccctcgtg
    20641 accaagcacc aggtgcccaa gttcatgggc gagagtgcgg tcccatgctg gtaggccctt
    20701 ctggatcaag aagacgtcgc gatcggcata ctggcgccac tgcccgcaga ccccaggggg
    20761 cagatcggcc atcttgagtt caatcgcccg gtcgcggtcc tcgctcacag catcgacaag
    20821 gcgaggcagt gacacttccc cgcgtcgtgg ggcgaggtcg agcaccgcac caacggcgcg
    20881 ggtgacacgg cggctggcag gcataaaaac cccctctcga cgagtccact atgcgctgtc
    20941 cgccctttca gggtgggcag tcttgtcagt tcacaaaccg tgcagtgcca cttgtgtgta
    21001 ccgcaaagcc cgccttcgcc tggcgatatc caactaatcc cccggcgccg gtgagtacaa
    21061 gcatgcccgc aacgccgggc actgccaagg ccgctacctg cggcaatccc gcactccgca
    21121 aatattcgga gtagccgacc cggtatgacg ccgcggggac cgctacgttg ctgcccacgg
    21181 aggccggcag cggctcccgg cccgcaggtg gttcggcgtt cacgccgcga ggtgcggcgg
    21241 gcaacgccgg tccggcgggt gcgccggccc cgccgcctcc agctccaaga cccgcgggca
    21301 gcgccacgat gaccggcaac gtgaggggcg gaatcggcag ctcgacggcc gctgcgccga
    21361 cgcccgggac gacgtcgatg accgacggcg cgcccggttc ggtcgcgggc ggcatgagct
    21421 cgggcggaag ctgcataggc ggcggcagcc caggtcggcc ggacggtact tcgggggcgc
    21481 taccgccacc accaccgcca ccgggtcccg gcggctcgcc tggcggccac ggccacggcc
    21541 acccgggcga gcagtggtcg tcgttcttct cctcgcactc gcccggatct tccggtggtg
    21601 gaggctcttc cggctttgtc gagggagtag tggtcttggt ggggggcgtc ggaggcgtcg
    21661 gatcatccgg atcggtcggg ttgccgcccc cgcagccgtg ggcgttcccg ttgccaccgc
    21721 tgtttccgca gttgccgttg cccgggtttc cgacaccgtt ctggccgttg ttgttatcgc
    21781 cgttctttcc gtcgccgtcg tcctgcttct gatcttcggg cttctgatct tcgggcttcg
    21841 ggtcggccgg atcctggtct tccttgtcgc caggatgggg tgtggtcttc ttgtcgtcag
    21901 gatcgaagtc cgacgagtcg ttggtgtgct tgccaccatg gccgccgtcg gcaagttgag
    21961 gatcggcaaa ggcgatagaa gcgccggcgc cacccgctaa cagcccggaa gcgacgaggc
    22021 aggctgctgc ggcggtgcgc acacgcgaac ccacgtcgtc accacccctc aaggcgtcac
    22081 cggccggcta ttcacaagtt ccggcaattc ttgcacaacc acgggccacg ggtttgctgt
    22141 ggctctgaac gtacccctga ccggggcaca tcgatgccgc agatagtccg aacgatagtg
    22201 ttggaccgca gcatgactgc tttgtcatga atcgtcaacg agatgaccgg gaggccgccg
    22261 ggatgagcct gttcaagcgc aaaacccgcg ccacccgcag agccgaggcc cgagccatca
    22321 aggccaaggc caaactcgag gccaagctcg ccgctaagaa cgaagcccgc cgaatcaaga
    22381 cacaacacaa ggccgagacc agggcgctca aggcccaact caaggcgcag cgcgacagcg
    22441 acagggcggc gctcagggtc gccgagacgc agctgaaggc ggcccgcgaa ggtaaattgt
    22501 tctcccccac gcggattcga cgtgtgctca ccgtcacccg gctgttagca ccaatcgcgg
    22561 ttccgctctt ctatcgcgcc gccatcgccg cgcgtggctt cctcgacgaa cgccgtgctg
    22621 accggctcgg cgtgccgctc agccagctcg gccagttctc ggggcacggc gctgcgctgt
    22681 cggctcggat cgccggtgcg gagcagtcac tgcgcctggt cgccgagaag aagccgaagg
    22741 acgccgagac caagcagttc gtcgccgcga tcaccgaacg gctgcgtgac ctgtcggccg
    22801 ccgtcgccgc ggcggaaaac atgccgacgt cgcgccgccg cgccgcacac gcagcgattg
    22861 cccaacagct cgatggcatc gacgccgacc tgatggcccg tctcggcgtg gcctaagacc
    22921 tgtcgagcat gagtcgtctt ctcgcggcat tggtcctggc catctcgttt accgtcagtg
    22981 cggtggccgc ggccggtgtc gcctcggcgc acgcgacccg cgtcgcgacc gatccggtcg
    23041 agaacactga gctgacccag tctccgcaga aggtcagcgc gatgttcagc gaggcgctgc
    23101 aacccgagtt cgccgcgatg acggtcgtcg ggccggacgg caacctgtgg tcgacgggtg
    23161 atccgcaggt ggacggcgcc gtcgtcagtg tcggcgtgcg gccgctcggc ccgtccggca
    23221 cctacacggt gaattaccgg gtgacgtcgg ccgacgggca cgtggtctcc gggtcgtggt
    23281 cgttccggct cgcagtgtcc ggcacgggca cacctggtcc gtcggcgtcc gcggcgagcc
    23341 cagcgagtga cggaattccg gtgtggccgt tcttcgtcgc ggcgggcctg atcgtcgccg
    23401 gtggcgcggt ttgggcggtg cgacgccaga ggtgacgcgc cgacgggcgg tcgccggtgc
    23461 agccctgatc gtcgctgcct ccgtcgtgct ggcgtgcatg ctggccggct cacagaatcc
    23521 gccggcagcg acgctggtgc gggcggtggc cgacggtgcg gcggtcgtcg cgatcggcct
    23581 ggccgcggtg ccgatgcttg acgtcgaccg ctaccgcggt gagctgattc gccgcgcgac
    23641 cgggccgttg acgatcgcca gc
//
LOCUS       DASRJA010000531        13222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_941070,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000531 DASRJA010000000
VERSION     DASRJA010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13222
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941070"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VFC01_23295"
     CDS             complement(<1..1178)
                     /locus_tag="VFC01_23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN82575.1"
                     /translation="MTGVQTESGVLAGDERMLIDGELQITSSKATFDVIHPASEQVAG
                     QATDGTVEDMARAVGAARRAFDKTDWSRDLEFRYHCLTQLHDALERNKERLRRVLITE
                     VGCPVTVTGSQIESPIAEVKHWAEHGRAFEYLVDNGLHETPLGPARRKIHYEPVGVVG
                     AITPWNVPFYLNVAETVPALMAGNTVVLKPAQLTPWSGSEYGRIVAEETDIPAGVFNV
                     VVSNANEVGAALSADPRVDMITFTGSTATGRAILAAGASTVKKTLLELGGKSAHVVLD
                     DADFNSALPMAAMMACVMSGQSCILPSRILLPRSRYDEGLAILKGAMENFPVGDPWTP
                     GNMQGPQISETQRQKVLGLIKSGLDSGARLVTGGGIPEKLPTGYYVQPTLLADVDPDS
                     "
     gene            complement(1211..1756)
                     /locus_tag="VFC01_23300"
     CDS             complement(1211..1756)
                     /locus_tag="VFC01_23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82576.1"
                     /translation="MNEPLRASEGVEDTSTRHRILVATAEVLARSGQTKLSLSEVALQ
                     AGVSRPTLYRWFASKGELLDAFGVFEREMFDSGISRATSGLRGTEKLDAALRFIVEYQ
                     QSYSGVRLVDIEPEVVIAQLGRILPMMRARLQKLMPGPNGAVKAATAIRVAVSHYIVR
                     SDDGDQFLAQLRHAAGIKQNG"
     gene            complement(1802..1930)
                     /locus_tag="VFC01_23305"
     CDS             complement(1802..1930)
                     /locus_tag="VFC01_23305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82577.1"
                     /translation="MFDRSMREWAACTPRTSQWKDWGEVSPPLDDLDTVALLEKSV"
     gene            2070..3236
                     /locus_tag="VFC01_23310"
     CDS             2070..3236
                     /locus_tag="VFC01_23310"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN82578.1"
                     /translation="MAGPLEGLKVVELGVWVAGPAAGGILADWGADVIKIEPPTGDPA
                     RMFGRMLGIEDGVSPPFEMDNRGKRSIVLDVTTDEGRATASELLSNADVFLTNVRPAA
                     LRRTGLDFETVASRNPRLVYGLITGYGETGPDADRAAYDVAAFWARAGLAHLLTRPGE
                     APPFQRGGMGDHTAGMTLAAAVCAALVARTRTGKGQLVSTSLYRQGAYTVSFDLNTFL
                     MTGHSIAIGQRETMGNPCMNNYTAGDGRRFWIVGLQGERHWPALCRAVGRPDWLDDPR
                     FSTGRSRAANAVDLIGELDEIFATRPLEEWAKAFAQEPDFFWSPINSIEDVMADEQFH
                     AAGGVVYVPDGDAGASMVATPADFHGTPWHPRSAAPRLGEHTGEILAELTGRSA"
     gene            complement(3217..3741)
                     /locus_tag="VFC01_23315"
     CDS             complement(3217..3741)
                     /locus_tag="VFC01_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82579.1"
                     /translation="MQQVDTSSRERILAATAEVLGRNGMSKLSLSEVALQAGVSRPTL
                     YRWFSDKRELLDAFVVWERQYYERAVADATTDLPACERLDAALRVIVEYQQSYPGLRM
                     IDIEPAQVIRRLARVIPMMRQRLERLATGPDPALAVATAVRVAISHYLVRSDDDDDFL
                     NQLRHAARVKHYAP"
     gene            3786..5039
                     /locus_tag="VFC01_23320"
     CDS             3786..5039
                     /locus_tag="VFC01_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN82580.1"
                     /translation="MTAATVQEREYSPIDITSYDFWSQPFAVRDETFAQLRAGEGLTW
                     HRAFPSMFPMEEPGYWALTRRADIAYVSQHPELFTSAQGVALDPMPAEIQHFASFFLT
                     MDPPQHTTYRRLISSAFTPRHVRQIEEQIHSSAVSIVDDLVGAGDIDFVEACSARLPM
                     LTIMEMLGVPKADQPGVAKAAEKLFGLSDDEYSTLEERAQDTINEIMLLNSTGQELAK
                     FRRTNPGDDLMTSIVNAEVDGHRLSDEEIGAFLILLASAGNDTTKQATTHSMMAMVEN
                     PAQRDWLMEDFEDRIGPAVEEFVRYSSPVLQFARFVTEDTEINGHPVSAGDKVGLFYC
                     SANRDEAVFEDPAAFNLQRSPNPHLGFGGGGPHFCLGNQLARTELRNLFRELLTRLTT
                     VEFGEPDLLHSSFVHGVKRVPAFVQ"
     gene            5048..5239
                     /locus_tag="VFC01_23325"
     CDS             5048..5239
                     /locus_tag="VFC01_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN82581.1"
                     /translation="MRVEVDLDKCTGHGICESIADDVFEVQDDGTVLIDENERPESDR
                     ERMQQAVTQCPAAALRLVD"
     gene            complement(5305..6114)
                     /locus_tag="VFC01_23330"
     CDS             complement(5305..6114)
                     /locus_tag="VFC01_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZN82582.1"
                     /translation="MSDSYYELVDGTDPLGEKFIATDMVRSTWSAAIQHAAPVSALLV
                     RGLERCEQRDDTRLSRVMVDLMGPVPAEGDFWVRTQRERSGKQIELLAAEMLAPGPDG
                     APRPVARASGWRLQTLDTADVVHAPAPPLRPVSEAKGRDMKKDWDPNYVHSLDWRWLT
                     KPLNDGPGESWIRPEVDLVKGETMTPLERLFAVADDANGIGTKLDIRRWTFLNTDLVV
                     HIHRVPDGEWIGIRAETNYGPDGIGTTIGTLFDESGAVGGIQQSVLVRPRR"
     gene            complement(6111..6923)
                     /locus_tag="VFC01_23335"
     CDS             complement(6111..6923)
                     /locus_tag="VFC01_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZN82583.1"
                     /translation="MTPLADGFCFGEGPRWFEGLLWFSDMLGEAVHTVNLQGAMTTLP
                     LPGHAPSGLGFRPDGSLLIASTEARQVLRYDGETVTTLVDLSDLAPANLGDMVVDDLG
                     RAYVGAQAFAGGVVVRIDPDDTATVVAKNLDFPNGMVITPDRTTLIVAESIGRRLTAF
                     TVSADGSLSDRRIFADGLDGPPDGITLDAEGAVWASMTLANQFERIVEGGIVTERIDL
                     GDRAAIACMLGGPERRTLFLLSSTEAYPQKLIGTKLSRLDAVTVDVPGAGLP"
     gene            7003..7737
                     /locus_tag="VFC01_23340"
     CDS             7003..7737
                     /locus_tag="VFC01_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HZN82584.1"
                     /translation="MKRYHSHTLLYLHETIELGSGRWGHLPAGGQGDDRFAEVFSDVY
                     HPMMKALGARLFAIWETTPYNGHWPQVTIIWEIDAFADYARIGKAQARGGSHEGPAAK
                     WSTYLSEIGASGEGRIMYAGKSNKTLAQLKESNFSAGLVIQEIMETKPGRQDDYIREL
                     ERLYVPWSERTGKRWLGSFITTFRFNEVIHYWALDGDWDCFENHYPSWKDSPPAEIVT
                     WMSVAPALRDGWEDSILQALPSSPLQ"
     gene            7734..8963
                     /locus_tag="VFC01_23345"
     CDS             7734..8963
                     /locus_tag="VFC01_23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN82585.1"
                     /translation="MNTPVLQDFSYDPFDPAVMADPLPYYRVLRDQHPVYYIDKWDTY
                     ALSRFADIWQVLEINDGTFVASEGTLPAATVLANHNAGPVPDPPLHPMPFHANFDAPI
                     YDTVRRCTSSQFRPKSVAGLADRIRTLANERLDELLPRGSFDLTQEYGGIVAASVVCE
                     LVGLPVDLAPEVLATVNAGSLAQPGSGVEVANARPGYLEYLTPIVQRRRSDGADGELP
                     IVDNLIGYRLPDGSALTDMEAAVQMLGVFIGGTETVPKIVAHGLWELGRRADQMASVR
                     ADLDANLPFAREEMIRYCAPAQWFARTVRKPFTIHDTTIHPGQRIITLLGSASRDDRE
                     YPDAEEFIWNRPIERLLAFGRGQHFCLGVHLARLEITIMVTEWLKRVPEWRIVSEAAS
                     RPPSSFQWGWNNIPVEV"
     gene            8966..9934
                     /locus_tag="VFC01_23350"
     CDS             8966..9934
                     /locus_tag="VFC01_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN82586.1"
                     /translation="MWSYRLVAPYTVERTEIPEPSPGSLDDGQVLLRFLAAGICGSDL
                     PGFRGTKGRLPGDTGLCAAEMNGFPIHEVVGEVMASRHPAHRQGDRVVGWASGFDGLM
                     ERVVADGNGLTPYDPGLSPQLAVGLQPLACVLYAVEQLPDLRGRRVAIIGQGSIGLLF
                     SYVAKAAGAEHVTGVDPIDRDETGKLFGVDTIVRSTSDRWVSHLKPDDRSDIVIEAVG
                     HQVATLSHAIEAAAFGGTVFYFGVADDDSYPISMRTMLRNNLTLKSGVTLDRRRVLEA
                     ADGFAREHPELLTNYLTHTFRVDEVQAAFELACRPTAERIKIAIVE"
     gene            9931..10680
                     /locus_tag="VFC01_23355"
     CDS             9931..10680
                     /locus_tag="VFC01_23355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZN82587.1"
                     /translation="MTTPSRLQEALAAKQRIWGGWVVGPTIIGPEEFAQAGYDYVGFD
                     VQHGYLDDADVALLLRRLEHVPIATAVRLPSADPAPIGRVLDAGADAVIVAMIESAEQ
                     AAAAVAATRFAPAGVRSFGPLRASLGHDPGALAARVSVFAMVETAQGLLALDDICTVP
                     GLTGVYVGPADLAISMGYTLAEAWTATDVLDAYARIHATASAAGLVTGIHAGVGKRGK
                     AMAELGFRMITLASESQALRRGAAEFLREAQ"
     gene            10677..11411
                     /locus_tag="VFC01_23360"
     CDS             10677..11411
                     /locus_tag="VFC01_23360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN82588.1"
                     /translation="MTSTDRVALVTGAARGQGAAIVKRLHKDGFRVAACDLLVDELAA
                     TVESVGGDAVIAIPLDVTSQDQWQAAVAAVIDRFGALTTLVNNAGVLHRASIADETAA
                     GFEGSWRVNCLGPFLGIRAAFPYLRKADGAAIVNTCSTGAIRPFPNHAAYGSSKWALR
                     GLTQVAAAELASSGIRVNAVFPGPIETPMLDDATQARLAERAVLGRLGKPMEIADAVA
                     FLVSEQASFITGSELVVDGGQCLQIG"
     gene            11393..12919
                     /locus_tag="VFC01_23365"
     CDS             11393..12919
                     /locus_tag="VFC01_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZN82589.1"
                     /translation="MPANRLSGNGLSVGIIGAGPGGLALGIFLRRAGFRDFTIFDRED
                     GVGGTWRINTYPGLACDVKSHLYSYSFDLNANWSRLWSAQPEILEYFEQCAQRYELGP
                     NLKLHTEIVSAQWDSESATWQLTTAAGEQYTFDIVVSAVGLFTQPVLPDLVEEEKFTG
                     TQMHTARWDHSIDLRDARVAVLGTGSTASQLIPEVAKEAKKVYSVQRSPTWILPKPDR
                     PYTDREKWLFAHVPFAKKIYRTRLWLRSESNISVIENGSDKTQEFKGIALKVLESTVA
                     DDELRRKLTPDHPFGCKRLVFASDYLQTLTQPHVEVVSSPARSLRSRSLVTEDGTELD
                     VDVVLCATGYAAADYLGQIDVVGEDGMSLREKWSDGAHAYLGMAVPGFPNFFMLYGPN
                     TNVGSNSVIFMLEAQARYIVRALSYMRRKRKAYVAVRPSAMDDFIAKIDEWMAGTVWL
                     TKCSNYFRAANGRVVTQWPRSARAFWGMTRRFRPADYTFDPPPEHSAVEAGSQSAVSG
                     "
     gene            12926..>13222
                     /locus_tag="VFC01_23370"
     CDS             12926..>13222
                     /locus_tag="VFC01_23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN82590.1"
                     /translation="MFADRLDPPLRQFASARTDLSAGVLWVVRDSLNQRRAQTARDVN
                     TVGVEIENREVGSIPVRIYRGGPSPAPAVIYCHSGAFVLGNLDTDHRQCVEFARR"
BASE COUNT         2305 a         4319 c         4293 g         2305 t
ORIGIN      
        1 tgtgaatccg ggtccacgtc ggccaacagc gtcggctgca cgtaataacc cgtcggcagc
       61 ttctccggga tgccgccgcc ggtgaccagc cgggcacccg agtcgaggcc actcttgatc
      121 aggccgagca ccttctgccg ctgcgtctcg ctgatctgcg ggccctgcat gttgccgggt
      181 gtccacgggt cgccgaccgg gaagttctcc atcgcgccct tgaggatggc cagaccttcg
      241 tcatagcgac tgcggggcaa caggattcgg ctgggaagga tgcaactctg acccgacatc
      301 acgcaggcca tcatcgcggc catcggcagc gccgagttga agtcggcgtc gtcgagcacc
      361 acatgcgccg actttccgcc gagctccagc agcgtcttct tcaccgtcga cgcgcctgcc
      421 gccaggatcg cccggccggt cgccgtcgag ccggtgaacg tgatcatgtc gacccgcggg
      481 tcggccgaga gcgccgcgcc cacctcgttc gcgttggaga ccacgacgtt gaacaccccg
      541 gccggaatgt cggtctcctc agccacgatc cggccgtact cgctgcccga ccatggcgtc
      601 agctgcgccg gcttgagcac caccgtgttg cccgccatca gcgcaggcac cgtttcggcg
      661 acgttgaggt agaacgggac gttccacggc gtgatcgcgc cgaccactcc gaccggctca
      721 tagtgaatct tgcgtcgggc gggcccaagc ggcgtctcgt gaagcccgtt gtcgacgagg
      781 tactcgaagg cgcggccgtg ctcggcccag tgcttgacct ccgcgatcgg gctctcgatc
      841 tggctgccgg tcacggtgac cggacagccg acctcggtga tcagcacgcg ccgcagccgc
      901 tccttgttgc gctccagcgc gtcatgcagc tgggtcaggc agtggtagcg gaactccaga
      961 tcgcgcgacc agtcggtctt gtcgaacgct cgccgcgccg ccccgaccgc gcgcgccatg
     1021 tcctcgaccg ttccgtcggt cgcctgaccg gccacctgtt cgctggctgg atggatgaca
     1081 tcgaatgtcg ctttgctgct tgtgatctgc aattcgccgt cgatgagcat ccgctcgtca
     1141 ccggcgagca cgccggattc agtctgtact cccgtcatgg gaaacagctt tacaaactag
     1201 cgggaaaatg tcacccgttc tgcttgatcc ccgcggcatg gcgaagctgc gcgaggaact
     1261 ggtcgccatc gtcgctgcgc acgatgtagt gcgacaccgc gactcgaatc gccgtcgcgg
     1321 ccttgaccgc cccgttcggt cccggcatca gtttctgcag gcgtgcgcgc atcatcggga
     1381 ggatcctgcc cagctgcgcg atgacgacct cgggttcgat gtcgaccaac cgcacgccgg
     1441 agtacgactg ctgatactcg acgatgaacc gcagcgcggc gtcgagtttt tcggtgcccc
     1501 gcagtcccga cgtcgcccgg ctgatgccgc tgtcgaacat ctcgcgctcg aagacaccga
     1561 acgcatcgag caattcaccc ttggacgcga accaccggta cagcgtgggc cgcgacaccc
     1621 cggcctgcag cgccacctcg gagaggctca gcttggtctg gccgctgcga gcgagcactt
     1681 cggcggtcgc caccaggatc cggtgccgcg tcgacgtatc ctcgacgccc tcggacgcgc
     1741 gtaatggttc gttcacgtgc tcggcctcaa tcgtttggtc ttgatgatcc tagagcgttg
     1801 atcacacgga cttctccaag agcgcgaccg tgtcgaggtc gtccaggggc ggcgacacct
     1861 cgccccagtc cttccactgc gatgttctcg gcgtgcatgc cgcccattcc cgcatggagc
     1921 gatcgaacac gaccgacgac cgcgtcggtg gcgatacgtt ggacaccgcg gccgagatgc
     1981 aacgccaggc cggcgatcgg ggcaacgcga tccatcacgc aatgtttaca aaaatgccgt
     2041 gaattgtaaa gtcactgcgg gagaggacta tggctggacc gttggaaggc ctcaaggtcg
     2101 tcgaactcgg cgtgtgggtg gcgggtccgg ccgccggagg aatcctggct gactggggcg
     2161 ccgacgtcat caagatcgag ccgccgacgg gcgatccggc gcggatgttc ggccggatgc
     2221 tcggcatcga ggacggcgtc agcccgccgt tcgagatgga caaccgcggt aagcgcagca
     2281 tcgtgctcga cgtgacgacg gacgaggggc gggcaaccgc atccgaattg ctcagcaacg
     2341 cagacgtttt cctgaccaat gttcggcctg cggcgttgcg acgcaccggc ttggacttcg
     2401 agacggtcgc ctcccgcaac ccgcgcctgg tgtacggctt gatcaccggc tacggcgaga
     2461 cggggcccga cgccgaccgc gcggcctacg acgtcgccgc cttctgggcc cgggcgggct
     2521 tggcgcactt gctgacccgt ccgggtgagg caccgccgtt tcagcgcggc ggcatgggcg
     2581 atcacacagc gggcatgacg ctcgcggcgg cggtgtgtgc ggcgctggtg gcgcggacgc
     2641 gaaccggcaa ggggcagttg gtcagtacct cgctgtaccg gcagggcgcg tacaccgtca
     2701 gcttcgacct caataccttt ctgatgacgg gccattcgat agcgattggc cagcgcgaga
     2761 cgatggggaa cccgtgcatg aacaactaca cggctggcga cgggcggcgg ttctggatcg
     2821 tcggactgca gggcgaacgt cattggcctg cgctgtgtcg cgccgtggga aggccggatt
     2881 ggcttgatga tccgcggttc tcgaccggcc gctcgcgcgc cgcgaacgct gtcgatctca
     2941 tcggtgaact cgacgagatc ttcgcgacga ggccgcttga ggaatgggcg aaagcctttg
     3001 cacaagaacc ggatttcttc tggtcgccga tcaactcgat tgaggacgtc atggccgatg
     3061 agcaatttca cgcggcgggc ggcgtcgtct acgtgcctga cggcgacgct ggcgcatcga
     3121 tggtcgcgac gccggccgac tttcacggca cgccgtggca cccgcgttcg gccgcgccac
     3181 ggctcggcga gcacaccggc gagattctcg ctgagctcac ggggcgtagt gcttgacccg
     3241 cgccgcgtgc cgaagctggt tcaggaagtc gtcatcatca tcgcttcgca ctaggtaatg
     3301 cgaaatcgcc acgcgcactg ccgttgccac cgccaaggcc gggtcaggcc ccgtggcaag
     3361 ccgctcgagc cgttgtcgca tcatcgggat cacccgagcc agccgcctga tgacttgggc
     3421 cggctcgatg tcgatcatgc gtagccccgg gtacgactgc tggtactcga cgatgacccg
     3481 caacgctgcg tctagccgct cgcatgctgg caggtcggta gtcgcgtccg cgaccgcgcg
     3541 ctcgtaatac tgccgctccc acacaacgaa cgcgtcgagc agttccctct tgtcggagaa
     3601 ccaccggtag agcgtgggcc tggacacgcc ggcctgcagt gcgacctcgg agagactcag
     3661 cttgctcatc ccgttgcggc cgaggacttc cgcggtggcg gccagtatcc gctcgcggct
     3721 cgaagtgtcg acctgctgca ccgtccaagc tttacaaatt tacgctgaaa tgtcacgctg
     3781 aatcggtgac agccgcaacg gtgcaggaac gtgaatacag cccgatcgac atcacgtcgt
     3841 acgacttctg gagtcagcct ttcgcggtgc gtgacgagac gttcgcccag ctccgcgcgg
     3901 gtgaaggcct gacctggcac cgggcctttc cctccatgtt tcccatggag gagccgggct
     3961 actgggcgct gacccgacgc gccgacatcg cctacgtcag ccagcacccc gagttgttca
     4021 cgtcagcgca gggcgtggcc ctcgatccga tgcccgccga gatccagcac ttcgcatcgt
     4081 tcttcctgac catggatccg ccgcagcaca ccacataccg gcgcctgatc agttcggcgt
     4141 tcacgccgcg acacgtgcgc cagatcgaag aacagatcca tagcagcgcg gtcagcatcg
     4201 tcgacgacct cgtcggtgct ggcgacatcg acttcgtcga agcctgctcg gcccggctgc
     4261 cgatgctgac gatcatggag atgctcggcg tgcccaaggc cgaccagccg ggcgtggcca
     4321 aggccgccga gaagctgttc gggctcagcg acgacgaata cagcacgctc gaagaacggg
     4381 cacaggacac gatcaacgag atcatgctgc tgaacagcac cggtcaggag ttggcgaagt
     4441 ttcgacgcac caatcccggt gacgacttga tgacgagcat cgtcaacgcc gaagtcgacg
     4501 gacaccggtt gagcgacgag gagatcggcg cgttcctgat cctgctggcg tccgcgggta
     4561 acgacaccac caaacaggcc accacacact cgatgatggc gatggtcgag aacccggcgc
     4621 agcgcgattg gctgatggag gacttcgagg accggatcgg acccgccgtc gaggaattcg
     4681 tgcggtactc gtcgccagtg ctgcagttcg cgcgcttcgt caccgaggac accgagatca
     4741 acgggcatcc ggtgagtgcg ggcgacaagg ttggcttgtt ttactgctcg gcgaaccgag
     4801 acgaggcggt cttcgaggac ccggctgcgt ttaatctgca gcgctcgccg aatccgcacc
     4861 tcggcttcgg cggcggcggg ccccacttct gcctcggcaa tcagctggcg aggacagagt
     4921 tgcgaaacct gttccgcgag ttgctgaccc ggctgacgac agtcgagttc ggggaacccg
     4981 acctgctgca cagcagcttc gtccacggcg tcaagcgcgt tccggcgttc gttcaatagg
     5041 agagatcatg cgggtcgaag tcgacctcga taagtgcaca ggacacggga tctgcgaatc
     5101 tatcgccgat gacgtgttcg aagtgcagga cgacggcacc gtgctgatcg atgagaacga
     5161 acgccctgag tcggaccgcg agcggatgca gcaggccgtg acgcagtgcc cggcagccgc
     5221 gctgcgtttg gtggactagc gacgaaacgg tattccagca gagaaagtgc gagtagaggc
     5281 ctgcctgaat acagtttcgg cgaactagcg gcgaggccgc accagcaccg actgctggat
     5341 gccgccgacc gcaccgcttt cgtcgaacag cgtgccgatc gtcgtgccga tgccatcggg
     5401 tccgtaattg gtctcggcgc gaatgccgat ccactcgccg tcgggcacgc gatggatgtg
     5461 cacgacgaga tctgtgttca ggaacgtcca tcgccggatg tcgagtttcg tgccgatgcc
     5521 attggcgtcg tcggccacgg cgaacaaccg ctcgagcggc gtcatcgtct cgcccttgac
     5581 aagatcgacc tccggcctga tccacgactc gccggggccg tcgttcaacg gcttggtcag
     5641 ccatcgccag tccaggctgt gcacgtagtt ggggtcccaa tcctttttca tgtcacggcc
     5701 tttggcctcg gacaccggac gcagcggtgg tgcgggcgca tgaaccacgt ccgcggtgtc
     5761 cagtgtctgc aaccgccatc cgctggcccg agcgaccggt cgcggcgcgc cgtccggccc
     5821 cggcgccagc atctcagcgg cgagcaattc gatctgcttg ccggagcgtt cgcgttgggt
     5881 ccgcacccag aagtcgccct cggcgggaac gggacccata aggtcgacca tgacacggct
     5941 caaccgcgtg tcatcgcgct gctcgcagcg ttccaatccc cgtaccaata gggcggatac
     6001 gggcgcggcg tgctgaatcg ccgcagacca cgtgctgcgc accatgtctg tcgcgatgaa
     6061 cttttcgccc agtggatcgg tgccgtcgac cagttcgtag tacgagtcgc tcacggcagc
     6121 cccgcaccgg gcacgtcgac ggtgaccgcg tcgaggcgtg acagcttcgt gccgatcagc
     6181 ttctgcggat aggcctctgt actcgaaagc agaaacaggg tgcgacgttc cggcccgccg
     6241 agcatgcacg cgatcgcagc ccggtcaccg aggtcgatgc gttcggtgac gatgcccccc
     6301 tcgacgatgc gctcgaactg gttcgccaac gtcatcgacg cccacactgc accttcggcg
     6361 tcgagcgtga tgccatcggg cggtccgtcg agaccatcgg cgaagatccg gcgatcggac
     6421 agcgatccgt ccgcgctgac cgtgaacgcg gtcaaccgtc ggccgatcga ctcggcgacg
     6481 atcaacgtcg tgcggtcggg cgtgatcacc atgccgttcg ggaagtcgag gtttttggcg
     6541 acgacggtgg ccgtgtcatc ggggtcgatc cggacgacga cgccgcccgc aaacgcctgc
     6601 gccccgacgt acgcgcgacc gaggtcgtcg accaccatgt cgccgaggtt cgcaggcgcc
     6661 agatcggaca gatcgaccag cgtggtcacg gtttcgccgt cgtagcgcag cacctgccgg
     6721 gcctcggtcg acgcgatcaa cagcgatcca tcggggcgaa agccaaggcc cgacggcgcg
     6781 tggccgggca acggcagcgt ggtcatcgcc ccctgcagat tgacagtgtg caccgcctcg
     6841 ccgagcatgt ccgagaacca cagcaatccc tcgaaccagc ggggtccctc gccgaagcag
     6901 aagccgtctg cgaggggcgt caacgtcgca cctttacaaa tcacggccta agtgtcacgc
     6961 tcgctaggtg ccactgtcaa ctaccggcgg cgcgtcgtga acatgaagcg ctaccacagc
     7021 cacaccctcc tctacctgca cgagacgatc gagctggggt cggggcgctg ggggcacctc
     7081 ccggccggag gccaggggga cgaccgcttc gccgaggtgt tcagcgacgt ctatcacccg
     7141 atgatgaaag cgcttggcgc gcggctgttc gcgatatggg agaccacgcc gtacaacggg
     7201 cattggcccc aggtcacgat catctgggag atcgacgcgt tcgccgatta cgcccgcatc
     7261 ggtaaggccc aggcgcgcgg cggcagccac gaggggcccg ccgcgaagtg gtcgacctac
     7321 ctctccgaga tcggcgcatc cggtgagggc cggatcatgt acgcgggcaa gagcaacaag
     7381 accctggcgc aactcaaaga gtcgaacttc tcggccggac tggtcatcca ggagatcatg
     7441 gagacgaaac ccgggcgcca ggacgactac atccgtgagc tcgagcgcct gtacgtcccg
     7501 tggtcggagc ggaccggcaa gcggtggctc ggttcgttca tcacgacatt ccgattcaac
     7561 gaagtcatcc actactgggc actagacggt gattgggact gcttcgagaa tcactacccg
     7621 tcgtggaagg acagcccgcc tgcggagatt gtcacctgga tgagcgtcgc gccggcgttg
     7681 cgcgacggtt gggaggattc gatcctgcag gccctaccct cgtcgccgct gcaatgaaca
     7741 cgccggtgct ccaggacttt tcgtacgatc cgttcgatcc ggcggtgatg gccgacccgc
     7801 tgccgtacta ccgcgtactg cgcgaccagc accccgtgta ctacatcgac aagtgggaca
     7861 cctacgcgtt gtcgcggttc gccgatatct ggcaggtgtt ggagatcaac gacgggacgt
     7921 tcgtcgcctc ggagggaacc ctgcccgccg caacggtttt ggcgaaccac aacgccggac
     7981 cggtgcccga tccgccgttg catccgatgc cgttccacgc caacttcgac gctccgatct
     8041 acgacacagt gcgccgttgc acatcgagcc agttccggcc caagtccgtc gccggattgg
     8101 ccgaccggat ccggacgctg gccaacgagc gactcgacga gctgttgccg cgcggtagct
     8161 tcgacctcac ccaggaatac ggcggcatcg tggccgcctc ggtggtgtgc gaacttgtcg
     8221 gcctgcccgt ggatttggcg ccggaagtgc tggccaccgt gaacgccggc agcctcgccc
     8281 agccgggcag cggcgtcgag gtggccaacg cccgtcccgg ctatctggaa tacctgaccc
     8341 cgatcgtgca gcggcgccgc tccgacgggg ccgacggtga attgccgatc gtcgacaacc
     8401 tgatcggata ccgcctgccg gacggttcgg ccctcaccga catggaagcc gcggtccaaa
     8461 tgcttggtgt gttcatcggc ggaaccgaaa ccgtgccgaa gatcgtcgcg cacggcctgt
     8521 gggaactcgg ccgtcgtgcc gaccaaatgg cctcggtgcg agcggatctc gacgccaatt
     8581 tgccctttgc gcgcgaggaa atgatccgct actgcgctcc cgcacagtgg ttcgcccgca
     8641 ccgtgcgtaa gccgttcacc atccacgaca ccacgatcca ccccggccag cggatcatca
     8701 ccttgctcgg atctgccagc cgcgacgacc gcgagtaccc cgatgctgag gagttcatct
     8761 ggaaccggcc gatcgagcgg ctgttggctt tcggtcgggg acaacacttt tgcctgggcg
     8821 tgcacctcgc tcgtctcgaa atcaccatca tggtgacgga gtggctaaaa cgggtgcccg
     8881 agtggcgcat cgtcagcgag gcggcgtcgc ggccaccgtc gagcttccaa tggggctgga
     8941 acaacatccc ggtggaggtc tgacgatgtg gtcgtatcgc ctcgtcgccc cgtacacggt
     9001 cgagcgaacg gaaatccctg agccgtcgcc gggatccctc gacgacgggc aggttctgct
     9061 gcgcttcctc gcagcaggca tctgcggaag cgaccttccc ggcttccgag gtacgaaggg
     9121 acggctgccc ggcgacaccg gcctatgcgc cgcggagatg aacggctttc cgatccacga
     9181 ggtcgtcggc gaagtgatgg cgagccgtca tccggctcat cggcagggcg atcgggtggt
     9241 cggctgggcg tcgggcttcg acgggctgat ggaacgcgtg gtcgccgacg gcaacggatt
     9301 aacgccgtac gaccccggac tcagcccaca gctcgcggtc ggactgcagc cgctggcctg
     9361 cgtgctgtat gccgtcgagc aactgccgga cctacgagga cgccgcgtcg cgatcatcgg
     9421 tcagggttcg atcggcctgc tgttctccta cgtggccaag gcggcgggcg ccgaacacgt
     9481 cactggagtc gacccgatcg accgcgacga gaccggcaag ctgttcggag tcgacaccat
     9541 cgtccgttcg accagcgacc gatgggtgag tcacctgaag ccggatgacc ggtccgacat
     9601 cgtgattgaa gccgtcggcc atcaggtcgc cacgctgagc cacgcgatcg aggcggcggc
     9661 cttcggcggc accgtcttct acttcggcgt tgccgacgac gacagctatc cgatcagcat
     9721 gcgcacgatg ctgcgcaaca acctcacgct gaaatcgggt gtgacacttg accggcggcg
     9781 ggtactggaa gccgccgacg ggttcgcgcg tgaacacccg gagctgctca cgaactacct
     9841 cacccacaca ttccgagtgg acgaggtgca ggccgcgttc gaactggcct gcaggccgac
     9901 agcggaacgc atcaagatag cgatcgtcga atgacgacac cgagcagact gcaggaagcc
     9961 ctcgccgcaa agcaacgcat ctggggcgga tgggtggtcg gacccacgat catcggtccg
    10021 gaggagttcg cccaggcagg ctacgactac gtcggattcg acgtccagca cggctatctc
    10081 gacgacgccg atgtcgcgct actgctacgc cgcctcgagc acgttccgat cgccaccgcc
    10141 gtgcggctgc cgtccgccga tcctgctccg atcggcaggg tgctcgatgc gggcgccgac
    10201 gccgtgatcg tcgcgatgat cgaatcggcg gaacaagcgg ccgccgcggt ggcggctacg
    10261 cggttcgcac cggcaggggt gcgcagcttc ggtccgctgc gtgccagcct cggccacgac
    10321 ccgggggcac tggcagcacg tgtcagcgtg ttcgcgatgg tagagaccgc gcagggtctc
    10381 ttggcgctcg acgacatctg tacggtcccc ggcctgacgg gcgtgtacgt cgggccggcg
    10441 gatctcgcga tctcgatggg atacacgctt gccgaagcat ggaccgcgac cgacgttctc
    10501 gacgcatacg cacgcatcca cgccaccgcc tcggccgccg gtctcgtcac cggcattcac
    10561 gctggtgtcg gcaagcgcgg aaaggccatg gcagaactgg gctttcgcat gatcacgttg
    10621 gcgtcggaat cccaagcgct acgccgaggt gccgcggagt tccttcggga ggcgcagtga
    10681 cgtcgacgga tcgggttgcg cttgtgacgg gcgccgcacg cgggcagggt gcggcgatcg
    10741 tcaagcgact gcacaaggac ggattccgtg tcgccgcctg cgatcttctg gtcgacgaac
    10801 tggcggccac cgtcgaatcc gtgggcggcg acgccgttat cgcgattccc ctcgacgtga
    10861 cgtcgcagga tcaatggcag gccgcggtcg cggcggtgat cgatcggttc ggtgcattga
    10921 ccacgttggt caacaacgcc ggcgtcttgc atcgcgcctc gattgccgac gagacggccg
    10981 cgggtttcga aggcagctgg cgcgtcaact gtttgggtcc gttcctgggt atccgcgctg
    11041 cgtttccgta cctgcgtaag gccgacggcg ccgccatcgt caacacctgc agcacgggag
    11101 cgatccggcc cttcccgaac cacgccgcat acggctcgtc caagtgggca ctgcggggac
    11161 tcactcaggt cgccgctgcc gaactcgcct catccggcat cagggtgaac gccgtgttcc
    11221 ccggacccat cgaaaccccg atgctcgatg acgcgacaca ggcgcggctt gccgagcgcg
    11281 ccgtcctcgg ccggttgggc aagccgatgg agatcgccga cgctgtggcg ttcctcgttt
    11341 ccgagcaggc gtcgttcatc accggttcag agcttgtcgt ggacggaggg caatgcctgc
    11401 aaatcggttg agcggcaacg gattgtccgt aggaatcatc ggcgcgggcc ctgggggact
    11461 cgcgctgggc atcttcttgc ggagagccgg cttccgtgac ttcacgatct tcgaccgcga
    11521 agacggcgtg ggcggcacgt ggcgcatcaa cacctatccc ggcctggcct gcgatgtgaa
    11581 atctcatctg tattcctact cgttcgatct caatgcgaac tggtcacggc tgtggtcggc
    11641 tcagccggag atcctcgagt atttcgaaca gtgtgcgcag cgctacgagc tcggcccgaa
    11701 cctcaagctg cataccgaaa ttgtgtcggc ccaatgggat tccgagtcgg cgacatggca
    11761 gctgacgacg gcggcggggg agcagtacac ctttgacata gtggtttctg cggttggctt
    11821 gttcacccag cccgtacttc ccgaccttgt cgaggaagag aagttcactg ggacgcagat
    11881 gcacaccgcg cgctgggatc actccatcga cttgcgggac gcgcgggtgg ctgtgctggg
    11941 cacgggatcg actgcgtcgc agctgattcc cgaggtcgcg aaggaggcca agaaggtcta
    12001 ctccgtgcag cgctcaccga cgtggatcct gcccaagccc gaccggccct acaccgaccg
    12061 ggagaagtgg ctcttcgccc atgtcccgtt cgccaagaag atctatcgga ccaggctgtg
    12121 gctgcgcagc gagtcgaaca tctcggtgat cgagaacggc agcgacaaga cgcaggagtt
    12181 caaaggcatc gcgctgaaag tcctcgagtc gacggtggcc gatgacgagt tgcgccgcaa
    12241 gctgaccccg gatcatccgt ttgggtgcaa gcgactggtg ttcgccagcg actatctgca
    12301 gacgctgacg caaccgcacg tcgaggtggt gtcgagcccg gctcggtcgc ttcggtcgcg
    12361 gtcgttggtc accgaggacg gtactgaact cgacgtcgat gtcgtgttgt gcgccacggg
    12421 atatgcggca gcggactatc tgggtcagat cgacgtcgtt ggcgaggacg gtatgtcgct
    12481 gcgcgagaag tggagcgacg gtgcgcacgc gtatctcggc atggccgtac cgggttttcc
    12541 caacttcttc atgctctacg gcccgaacac caacgtcggc tccaacagcg tgatcttcat
    12601 gctggaggcg caggcccgct acatcgtccg agcattgagt tacatgcggc gcaagcgaaa
    12661 ggcgtatgtg gctgtgcgtc cgtcggcgat ggacgacttc atcgccaaga tcgacgagtg
    12721 gatggcgggc accgtgtggc tgactaagtg cagcaactac ttccgtgccg ccaatggccg
    12781 agtagtcact caatggccgc gcagcgcacg ggccttctgg gggatgactc ggcgatttcg
    12841 gcccgccgac tacacgttcg atccgccgcc cgagcactct gccgtcgagg cgggctctca
    12901 gtcagcggtt agcgggtagg cactcatgtt cgcggatcga ctggacccgc cactgcggca
    12961 gttcgcgtcg gctcggaccg acttgtcggc gggtgtgctg tgggtcgttc gtgattcgtt
    13021 gaaccagcgc cgcgcccaaa cggctcgcga cgtcaacacc gtcggcgtcg agatcgagaa
    13081 ccgcgaggtg gggtcgatcc cagtgcgaat ctatcgcggc gggccgtcgc ccgcgccggc
    13141 ggtgatctat tgccattcag gggctttcgt gctgggcaat ctcgacaccg atcaccgcca
    13201 gtgtgtggaa ttcgccaggc gg
//
LOCUS       DASRJA010000532        30143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_347453,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000532 DASRJA010000000
VERSION     DASRJA010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 30143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30143
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347453"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFC01_23375"
     CDS             <1..265
                     /locus_tag="VFC01_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZN82591.1"
                     /translation="DGFCSGLADALHGSGVRLLVIRPGFVIGRMTEGLAPAPFSRTAP
                     QVADATVRALGGKRSSVWFPWQVGALAVAMRLLPRPIWRRMRR"
     gene            262..1467
                     /gene="cbiE"
                     /locus_tag="VFC01_23380"
     CDS             262..1467
                     /gene="cbiE"
                     /locus_tag="VFC01_23380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HZN82592.1"
                     /translation="MIVVVGIGADGMSGLAQPSLAELRRATMVYGSRRQLDLLDDTVT
                     AERRQWPSPMLGVVETLGDVDGDVHVVASGDPMLHGVGGLLIRLFGADRVTVLPHVSC
                     VTLACARLGWAVQETEVISLMTSEPHTAMRRGGQAVVLCRDGAGPAALARLLTDGGRG
                     DSELTVLEQLGGPAERRRDATAREWAANPPDDVDDLNVVAVRYLPDDPRLSVLPDDAF
                     AHDGQITKQTMRAVTLAALAPRKGELLWDVGAGSGSIAIEWCRNGSRCRAVAFERDEQ
                     RRKRIAENALAHAAVVDVRGEAPAAFDDAVLSDRGVDGVPRPSAIFIGGGVTQLGLLE
                     ACFDRLPAGGRLVANAVTAESEAVLAQWYSRVGGELRRFQHYRGEPVGGFTGWRPALP
                     VTQWSVTKQ"
     gene            1464..2219
                     /gene="cobM"
                     /locus_tag="VFC01_23385"
     CDS             1464..2219
                     /gene="cobM"
                     /locus_tag="VFC01_23385"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HZN82593.1"
                     /translation="MTVYFIGAGPGAADLITVRGQRLLSRCPVCLYAGSIMPEDLLAL
                     CPPDARVIDTGPLTLDQIISELADAHAAGLDVARLHSGDPSIYSALAEQCRRLDALGV
                     DYEIVPGVPAFAAAAAVLGRELTVPGVAQTVTLTRVATLSTAMPPGEDLQTLAASGST
                     LVLHLAAAQIDAMVPQLLAGGYGPETPCAVVAFATWPQETVIRCTLSDLAEETTAAGV
                     TRTAVIIVGDVLAAEGFSDSYLYSSGRVRRGRH"
     gene            2219..2950
                     /locus_tag="VFC01_23390"
     CDS             2219..2950
                     /locus_tag="VFC01_23390"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HZN82594.1"
                     /translation="MRILLLGGTSEARALAAELHPDVEVISSLAGRVPEPALPVGEVR
                     IGGFGGVDGMRRWLSESGVDAVVDATHPYAATITAHAAAVCGELGLSHLVLARTAWDP
                     GDALVVGSDVEAAKTVAADGFSRVFLTTGRSGVKAFVDADAWFLIRAVTAPDAGTLPR
                     RHQLVLSRGPYHYEDELALLRDHGIDALVTKNSGGAMTRPKLDAAAALGVAVVMVDRP
                     PLPPNVVTVATVDEAADWVRSTGSC"
     gene            complement(2928..3992)
                     /locus_tag="VFC01_23395"
     CDS             complement(2928..3992)
                     /locus_tag="VFC01_23395"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HZN82595.1"
                     /translation="MPIPRHPGDLTSDWLSAVLAVEVKHVDVSPIGTGQTGATYRVSA
                     TYATDTDLPRSFAIKLPAQDDTVRERVALGYLSEVEFYTDVADRVRIPVPRCFHSDIS
                     ESGADFALLLADMAPAVQGDQIAGCTPQEAQLAVEALAGLHGPSWCDPTWLGLSAISM
                     PKPGDEAAAKGLGDVSVMAADITIEKLGARMGADDKETLRAAMGSVTPWLLAEPKRFA
                     LMHGDYRLDNMLFDPDRTRITVVDWQTMGIGLPARDLAYFTATSLAAELRADIEKDLV
                     GRYHAALLGYGVTDYDVETCWRDYRLGVIQAPLITGLGFAFAASTERGDEMILTMLHR
                     GCQAIRELETLDLINSYQSI"
     gene            complement(4045..5502)
                     /locus_tag="VFC01_23400"
     CDS             complement(4045..5502)
                     /locus_tag="VFC01_23400"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HZN82596.1"
                     /translation="MSRGTLWGVGLGPGDPELVTVKAAKVIAEADVVAYHSARHGRSI
                     ARGIAEPYLRPGQIEEHLVYPVTTETTDHPGGYAGAMEEFYRESADRIAAHLDAGRHV
                     ALLAEGDPLFYSSYMHMHTRLTERFDAVIVPGVTSVSAASAATGTPLVQGDEVLTILP
                     GTLPVDELKRRLADTDAAVVLKLGRSYPAVREALSSAGRLDETFYVERASTPKERVLP
                     AGEVDDASVPYFSLAMLPGAPRPQASTGSVAVVGLGPGDTDWMTRQSRRELAAATDLI
                     GYGPYLDRVGTRDGQCRHPSDNTDEPARARLACTLAQQGRAVAVVSSGDPGVFAMATA
                     VLEEAKQWPGVEVRVIPAMTAAQAVASRVGAPLGHDYAVISLSDRLKPWEVIEQRLTA
                     AAQADLVLAIYNPASKSRTWQVGAMRDLLLEYRDSSTPVVIGRDVSGPREEVKVVRLA
                     DLDPADVDMRCLLIIGSSQTQWYDDRVFTPRHYPT"
     gene            complement(5499..6125)
                     /locus_tag="VFC01_23405"
     CDS             complement(5499..6125)
                     /locus_tag="VFC01_23405"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HZN82597.1"
                     /translation="MLAYIRDAAEIYRQSFATIRAEADLSRFPDDVARVVVRLIHTCG
                     QVDVAEHVAFSEDVVAKSHAALAAGAPVLCDSSMVAAGITRSRLPGDNEVVSLVADTR
                     AAELAERTGTTRSAAAVDLWADRLGGAVLAIGNAPTALFRLLELVDDGAPTPAAVLGG
                     PVGFVGSAQSKQELIDRPRGMAYLVVQGRRGGSAMAAAAVNAIASEQE"
     gene            complement(6135..7253)
                     /gene="cobG"
                     /locus_tag="VFC01_23410"
     CDS             complement(6135..7253)
                     /gene="cobG"
                     /locus_tag="VFC01_23410"
                     /EC_number="1.14.13.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0019250 - aerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B synthase"
                     /protein_id="HZN82598.1"
                     /translation="MARTRDRDLCPGALQLHQAADGALARVRLPGGVISPAQLESLAH
                     TATRFGSPAMELTSRGSIQLRSLTDTAAVAEIIAAAGLLPSQTHERVRNIVASPLSGR
                     VGGSTDIRGLVTELDDAIQSEPALAELPGRFMFGVDDGRADISGLAADVGAHFVSEST
                     AALLLSGRDTGVRLNTSGVVAALVAVATQFAVTHGKAWRIAELADTAPLLQEFTPSAA
                     PGRTSPPVVRPPVGWLPQDDGRVALGAAVPLGVLQARVAEFLAAIDAPMAVTPWRSVL
                     VFDLDEGVADTALRVLAPMGLVFDGNSPWLSVSACTGSPGCEHSAADVRADAAAALDD
                     PAGGHRHFVGCDRACGSPPAGGVLVATGDGYRLRTPPP"
     gene            7496..7804
                     /locus_tag="VFC01_23415"
     CDS             7496..7804
                     /locus_tag="VFC01_23415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82599.1"
                     /translation="MTEVQAPFGAPPDAVEQTADVAETRTPVLITEQQVLFATAAALP
                     VRPAKARRRWTETVRGLAASLTAAFAASSNEARPKRQHYPARNDFLEDSRMAREMLRL
                     "
     gene            8070..8378
                     /locus_tag="VFC01_23420"
     CDS             8070..8378
                     /locus_tag="VFC01_23420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82600.1"
                     /translation="MTDMATGYRPELDKNDTSPVAPAANAFETQTLITEQQVLFSTAA
                     AVALPPTKTRRWSDAVLAIASAVGSWVASAAKPPTKPAYPKRHVWLENALMSREMDRL
                     "
     gene            complement(8379..9281)
                     /locus_tag="VFC01_23425"
     CDS             complement(8379..9281)
                     /locus_tag="VFC01_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HZN82601.1"
                     /translation="MVKLGANIGARVDGVRLAGDLDVETAAAINDALLKHKVIFFRDQ
                     HDLDDDGQLAFASKLGTPTTAHPTLSEGETVLPIDSRYDKANSWHTDVTFVDRIPKAS
                     LLRAVSLPNYGGTTAWASTEAAYEQLPAPLRVLTENLWAVHTNQYDYVREYDDRHEEL
                     ADTVREYREEFVREYYETEHPVVRVHPETGNRVLLLGHFIKQFVGLGPTESATLFNLL
                     QARVTKLENTIRWSWQPGDLAIWDNRATQHYAVADYDDQYRRLNRVTLAGDIPVDIHG
                     QRSRAVVGDASQYSDVVSPIALAS"
     gene            complement(9607..10011)
                     /locus_tag="VFC01_23430"
     CDS             complement(9607..10011)
                     /locus_tag="VFC01_23430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007531604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN82602.1"
                     /translation="MSETLTINGAVPIVPYSNPRDAIRWLEQTFGAVATAVHPPDPDQ
                     PLKHAEVKVGNGLVMINDADRDDASPFALPGPVLRYVVVDDPDALHDRAAAAGADIVM
                     GLTDQDYGSREFAARDPHGNVWSFGTYRPSLG"
     gene            complement(10022..10843)
                     /locus_tag="VFC01_23435"
     CDS             complement(10022..10843)
                     /locus_tag="VFC01_23435"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82603.1"
                     /translation="MSATFAAGTPHPSLRGVVLRYEGYEEQVAGPTTFRELPCTFVPV
                     IIDLDAGWSVAHRQHTTPLRLGSFVAGVTDGPVLVGHGGSARCLQVDLTPLGARQLVG
                     LPMSELANQSVPIDAVLGRFGRDLVQRVGDAPDWPTRFTLIDKVFRARLAEAAPADPG
                     VAWSLSQIVATGGTAVIGDLADELGWSHRRLIARYRDTVGLPPKLVARIVRFERLTAH
                     LTADPAIELAAAATACGYFDQAHLAREVRELADITPTELRATTVNSVQDEVAPSA"
     gene            10895..14467
                     /gene="cobN"
                     /locus_tag="VFC01_23440"
     CDS             10895..14467
                     /gene="cobN"
                     /locus_tag="VFC01_23440"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HZN82604.1"
                     /translation="MQEPTVLLLSTSDTDLITARASGARYRWANPSRLVDGELAELLA
                     GADIAVIRILGGYRAWQDGIDTVVASGMPTVVVSGEQSPDADLMGHSTTPAGVALQAH
                     VYLAQGGVPNLRQLHAFLCDTLLMTGFGFAPPETTPSWGVLQRASTGNDGPTIAVLYY
                     RAQHLAGNTAYVEALCHAIEEAGGRPLPVFCASLRTAEPELLDVLGSADAMVTTVLAA
                     GGATPAAVTAGGNDDTWNVAHLAALDIPILQGLCLTSSRAQWQDNDDGLSPLDVATQV
                     AVPEFDGRIITVPFSFKEIDDEGLISYVADPERCARVAGLAVKHARLRAIPASDKRVA
                     VVFSAYPTKHARIGNAVGLDTPASAIALLRAMGDAGYVIGDVPGVDAEDGDALIHALI
                     ERGGQDPDWLTDRQLSGNPIRLPAKEYREWFATLPHELTDAVVKHWGPPPGELFVDRS
                     RDPDGEIVIAALQDGNLVLIVQPPRGFGENPVAIYHDPDLPPSHHYLAVYHWIDAGFS
                     ADAVVHLGKHGNLEWLPGKTLGMSSACGADAALGNLPLIYPFLVNDPGEGTQAKRRAH
                     ATLVDHLIPPMARAETYGDIARLEQLLDEHATIAALDPNKLPAIRQQIWTLMRAAKMD
                     HDLGLDERPEDDSFDDMLLHVDGWLCEIKDVQIRDGLHVLGQKPTDSVELDLVLAILR
                     ARQLFGGDQTVPGLRQALGLAEDGNDDRATVDTAEARARELVAALQETGWDAAAVDTL
                     TDDPVIAGILRFAATEVVPRLAETAGEIGQILRALDGRFIASGPSGSPLRGLVNVLPT
                     GRNFYSVDPKAVPSRLAWETGVAMADSLLDRYRADYGHWPQSVGLSVWGTSAMRTAGD
                     DIAEVLALLGVRPIWDDASRRVVDLEPIPLAELGRPRIDVTVRISGFFRDAFPHVVTM
                     LDDAVHLVAGLDEPADDNYVRAHAQADLAEHGDVRRSTTRIFGSKPGTYGAGLLQLID
                     SRNWRDDADLAQVYTAWGGFAYGRGLDGAPAADDMNRQYRRIAVAAKNTDTREHDIAD
                     SDDYFQYHGGMVATVRALTGQSPAAYIGDNTRPDAVRTRTLSEETTRVFRARVVNPRW
                     INAMQRHGYKGAFEMAATVDYLFGYDATAGVMADWMYERLTQEYVLDDENRKFMAESN
                     PWALHGMAERLLEAAGRGMWAQPEQATLDGLRAVLLETEGELEG"
     gene            14477..14857
                     /locus_tag="VFC01_23445"
     CDS             14477..14857
                     /locus_tag="VFC01_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZN82605.1"
                     /translation="MPVTFAEVAKSEYILLTTFTKDGRPKPTPIWAAPDGDRLVVITQ
                     EQSWKVKRIRNTPRVTLAACDMRGNPKSETIDAAASILDKSANGATYDAIGKRYGLLG
                     KTFNVFSKLRGGMKNNVTIELRAA"
     gene            14861..15247
                     /locus_tag="VFC01_23450"
     CDS             14861..15247
                     /locus_tag="VFC01_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZN82606.1"
                     /translation="MADTFADVAGSKYIRLTTFTKDGRPKPTAVWGAPDGDKLLVITD
                     DGSWKTKRIRNTPRVTIQRSGGLGRPKGEPVEAVARVLPKDQTRKVYDAVVKRYWWHA
                     WWFIPHSLVRGGIDKVHIGLEIKPAS"
     gene            complement(15231..15935)
                     /locus_tag="VFC01_23455"
     CDS             complement(15231..15935)
                     /locus_tag="VFC01_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZN82607.1"
                     /translation="MKTIQIDTPAGPIDALLSVPDGGGPWPGVVVVHDAIGYAPDNES
                     TSARIAAAGFVAITPNLYSRGGRTRCITRVFRELLSQRGRAVDDILAARDHVQSMPEC
                     TGSVGVVGFCMGGQFALVMSPKGFGASAPFYGTPLPRHLSETLDGACPIVASFGRRDP
                     LGVGAPARLRKVVDHKGIAADIKVYPDAGHGFANKLPNQPLLRITGFGYNDAATEDAY
                     RRVFAFFNEHLASSRA"
     gene            15985..16467
                     /locus_tag="VFC01_23460"
     CDS             15985..16467
                     /locus_tag="VFC01_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HZN82608.1"
                     /translation="MAMRLFVLYVVVELAVLVALTSTVGFGWTVLALLGTFIVGLALA
                     GSQVKRHIRRLQSGLTAAKVQGAAADSMLVALGTVLVVIPGLASSVLGALLLLPPTRA
                     AARPLVTAMAARRVPLIAAAGAAAPGWGYDAAGRGDFIDGEVIDVVDVKPQAVERKPE
                     "
     gene            16469..18055
                     /locus_tag="VFC01_23465"
     CDS             16469..18055
                     /locus_tag="VFC01_23465"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZN82609.1"
                     /translation="MTTLLLNGRVHSPAMPDATAMAVTDGIVAWLGRDDVGRAQYPDA
                     EIVDLDGAFVAAAFVDSHVHLTATGLTLSGLDLRHATSLRHCLDLIAGYVRAHPDGPI
                     WGHGWDESGWPAHTAPSTDDLDGVVGDRPAYLARVDVHSAAASTALRRLAPGLAGAAG
                     FDPQRPLTSDAHHLVRAAARGRLTPAQRHEARLAALDAAAALGIVAVHECAGPDIGGI
                     DDWSEARDTEHGVDIVGYWGEHVTTAEQARELIDRTGARGLAGDLFVDGALGSRTAWL
                     HQPYHDAPHCRGNAYLDVDAITAHLRACTDAGVTAGFHVIGDAAVTAVVTALERVVDQ
                     FGAPAVARCGHRLEHLEMVTEEQAARLGAWGVFASMQPNFDALWGGEDGMYVQRLGSD
                     RGMKLNPFALLASQGVPIAFGSDSPVTGLNPWATVRAAIRHRTPGSAVSARAAFAAAT
                     RGAWRAGGARDGVIGTLVPGAPASYAVWEADELEVSAPADAVQRWSTDQRSRVPALPR
                     LEDPLPRCRQTVHRGAVIHG"
     gene            18234..20561
                     /gene="lnt"
                     /locus_tag="VFC01_23470"
                     /pseudo
     CDS             18234..20561
                     /gene="lnt"
                     /locus_tag="VFC01_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016719387.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
     gene            complement(20598..20936)
                     /locus_tag="VFC01_23475"
     CDS             complement(20598..20936)
                     /locus_tag="VFC01_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HZN82610.1"
                     /translation="MADRVLRGSRLGAVSYETDRNHDLAPRQVARYRTDNGEEFDVPF
                     ADDAEIPHTWLCRNGMEGTLIEGDAPEPKKTKPPRTHWDMLLERRSVEELEELLKERL
                     DIIKTRRRGS"
     gene            21059..21388
                     /locus_tag="VFC01_23480"
     CDS             21059..21388
                     /locus_tag="VFC01_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82611.1"
                     /translation="MARRLKPQPCRWCGREVADAGLGRRRQYCRQSCRQRAYEQRAVV
                     KGTSLAPDSVVLTADEASELSDRVYQVRCAAEDVAIAVEEGAEGTELRQLCDALMQAA
                     RAADGWR"
     gene            21500..22687
                     /locus_tag="VFC01_23485"
     CDS             21500..22687
                     /locus_tag="VFC01_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN82612.1"
                     /translation="MWRFDAFVLDTQRYELRSGDEVIRVEPQVFDVLTELVRNSHRFV
                     TKEELFDSVWGGRFVGEAALTSRIKAARRALGDDGESQRYIRTVRGRGYQFVGTIHAD
                     AAAEPEAPPEPASPRQHIAFCRAADGVRLAYAVAGEGPPLVRAANWMTHLGYDVESPV
                     WKHWVRDLSLKYRFIRYDERGCGLSDWDATDFTFDDWVDDLESVVETLGLERFPLLGV
                     SQGGAVAVAYAARHPERVSRLVLCGAYARGRGVRAVGNEEKRAAALDLELARVGWGRD
                     DPAFRQVFAAQFQPDGTRADWAAFDQLQRRTTSPENAVRFLEEFGRIDVRDLAREVSC
                     PTLIMHSRDDHRVPMRYGEELAALIPDSRLVALASNNHLLTAAEPAWPVFLAEVDAFL
                     AAE"
     gene            22776..23489
                     /locus_tag="VFC01_23490"
     CDS             22776..23489
                     /locus_tag="VFC01_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82613.1"
                     /translation="MRKHFRTLLVGVSALLALGTLSACSGGKTTEATNTSEAVTTPSA
                     TAQPFPQSARYVADMKAADGKTMTIGISVDGASIAAYACNGTDDEAWFFGNQADGKID
                     IKSRFRDTLKAEFTGTDVAGDLTMNGVAYTFTAAPVSGVAGMYTADFEGVRASWVVRE
                     DESAIGVQFNTGSISGRDFEQAELQQLNEQQFRSGVRNKRQIQQAAQIQFLQNKTARS
                     TINNQEVTPTLVNGTFRLG"
     gene            23645..24442
                     /locus_tag="VFC01_23495"
     CDS             23645..24442
                     /locus_tag="VFC01_23495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010840254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN82614.1"
                     /translation="MAVPLASATAAINRRRTHRMWGLIRLLSPVVLLGVWQLGSALGV
                     IPQDVLPAPSLIVEAGIELIESGQLADALQVSGVRVIEGLLLGGLLGVALGTAVGLSR
                     WFEATVDPPMQMIRALPHLGLIPLFILWFGIGELPKVLLVALGVSFPLYLNTFSAIRQ
                     VDPKLFETAQVLGFSFWQRFRTIIVPSAAPQVLVGLRQSLAIAWLTLIVAEQINADKG
                     IGFLINNARDFLRIDIIIFGLIVYALLGIGTDAIVRGLERRALRYRT"
     gene            24439..25161
                     /locus_tag="VFC01_23500"
     CDS             24439..25161
                     /locus_tag="VFC01_23500"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN82615.1"
                     /translation="MTIASERQTVVAGELRHVNKWYGDHHVLTDVSVRVARGEIVALI
                     GRSGSGKSTVLRVFAGLSTDHTGERVVSGAPALAFQEPRLFPWRDVRTNVVYGLTRSR
                     LPRAEAQTRADRALADVGLGDRADVWPLTLSGGQAQRVSLARALVAEPQLLLLDEPFG
                     ALDALTRLSMRGLLLDLWREHGFGVLLVTHDVDEAVALADRVLVLEEGRVIHTLAIDD
                     PRGSAHTEQYRAELLDKLGVQI"
     gene            25183..26199
                     /locus_tag="VFC01_23505"
     CDS             25183..26199
                     /locus_tag="VFC01_23505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN82616.1"
                     /translation="MHKLVAVIALLGLVLTGCVSREDSSGAKEAPATVPLSELSDLTL
                     QVGDQKGGTESLLRAAGALDNLPYKIAFSTFTSGPPQVEAATAGKIDFAITGNTPPIF
                     GAASNAKVKVVSAYDGGGNGDQILVHADSPIHSIADLRGRSVAVGKGSSANGHILAQL
                     KKAGLSPSDVKLVFLQPADALSAFTQRQADAWAIWDPYTAQAAEQLPVRSIGQARDVT
                     NGNWFGIASDAALADPRRNTALADLLVRFEKAARWAKDHPQRWAERYAAAVGLDVKVS
                     EVAQGRSLRLPTELDDDIIASEQKIADLFAESGQIASPAPKFADWVDRRYNETLRPLL
                     ISSN"
     gene            26209..27327
                     /locus_tag="VFC01_23510"
     CDS             26209..27327
                     /locus_tag="VFC01_23510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN82617.1"
                     /translation="MPTSSESPAQRAKFFWFLPTNGDSRSIVGASHASSHHTVPANYR
                     APNRRYLAEVARAADRLGYEGVLTPTGTWCEDAWLTASALLAETERLKFLVAFRPGLV
                     PPTLAAQQTATLQRFSEGRVLLNIVSGGDDLEQRRFGDWLSHDERYARTGEFLHIVNS
                     VWQQDSVDFEGKYYTVADARVSDPPDPLPQIYFGGSSAAALPIAVQYVDVYLTWGEPP
                     QAAAAKIEQVRKLATERGRNVRFGIRLHTISRDTSAAAWAVADELLAELTSEQISKAT
                     ELHAKSESEGQRRMTALHGGRVDQLEIYPNLWAGVGLVRGGAGTALVGSHEEVANLIY
                     EYHSLGFDEFILSGYPHLEEAYWFAEGVLPLLKQKGVA"
     gene            27355..27927
                     /locus_tag="VFC01_23515"
     CDS             27355..27927
                     /locus_tag="VFC01_23515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82618.1"
                     /translation="MDEWFERSPFIGFAPWIIYWVVSDGPSTWMFGAICAVIAALTLG
                     VSAGRGGPRILEVVTIVFFVAVTVGGMIVGADDRDWMDTYATTLSSGVLALMSLGSLA
                     FVPFTAQYAPESAPQQDWEKAAFRRTNRVLTLMWALVFALIAVLGYVAVTAPATIGWT
                     KAVIPVVVIIGAVGMTQIYPTRARENARPA"
     gene            27948..28331
                     /locus_tag="VFC01_23520"
     CDS             27948..28331
                     /locus_tag="VFC01_23520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN82619.1"
                     /translation="MNADKQVCGRRLADEQADLVVEVFRMLSDATRVQLLWALADREL
                     SVNDLAGRVGKPGPSVSQHLAKLRMARLVRTRREGTQVFYRLENDHVRQLVTDAVHNA
                     EHAAGGMPAHHLDDAELRLLHEGAG"
     gene            28331..29257
                     /locus_tag="VFC01_23525"
     CDS             28331..29257
                     /locus_tag="VFC01_23525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZN82620.1"
                     /translation="MGIAHSHDHTHSVDDALRDSTAGIRAVKISLVVLGCTATAQLVI
                     VALSGSVALFADTVHNFADALTAVPLWIAFSMSRRRATRRYTYGFGRVEDLAGLFVVA
                     VIATSAVVAAVQAIRRLIDPVPLHHLAWVAAAGVVGFLGNEMVALYRIQVGKRIGSAA
                     LRADGMHARADGLTSLAVVAGAVGAALGFPVADPIVGLVVAGAIGIVLIFAARDVFGR
                     LLDRVDPDLVDNAVTVLAARPGVRAVRRVRMRWVGHQLEADAELDVDPSLSLADAHSV
                     AHGAEHELAHAIPKLGSVIVHAYPAHDGVLSS"
     gene            complement(29247..29936)
                     /locus_tag="VFC01_23530"
     CDS             complement(29247..29936)
                     /locus_tag="VFC01_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82621.1"
                     /translation="MRIAALIAASVLVAGCSQSVGGDAERTSPSLTAPPSTTRAAPTT
                     TPPTSTTPAKAPGPGAPMSDVIAWVQAGTPSDPAQYHSATRDGAATELGEDVAFVTPS
                     GKTSCMTDTKYSGGALACLVELKNPPPRPDDAYGEWKGGWIDYDGLSVRVGSVHGDPG
                     RFTAGSGPELPYGHSLTFGDFRCRSDQAGLVCVNYAHQSGARFSDVGVQPFGCLQPAT
                     APPQIGEMFSC"
BASE COUNT         4896 a         9989 c        10189 g         5069 t
ORIGIN      
        1 ggacgggttc tgcagcggtc ttgccgacgc gctgcacggc tcaggggtgc ggttactcgt
       61 catccggccg ggattcgtga tcgggcggat gaccgagggc ctggcgcctg cgccattctc
      121 caggacggcg ccgcaggtgg cggacgcgac cgtgcgcgca ttaggcggga agcggagcag
      181 cgtctggttt ccatggcagg tcggcgcact cgcggtagcg atgcggctgt tgccgcggcc
      241 catctggcgg aggatgcgcc gatgatcgtg gtggtgggta tcggggcgga cgggatgtct
      301 gggctagcgc agccttcgtt ggccgagttg cgcagggcga caatggttta cggctcacgt
      361 cgccagctcg atctgctgga cgacaccgtg accgcggagc gcaggcagtg gccgtcgccg
      421 atgctgggcg tggtcgagac cctgggggac gtcgacggtg acgtgcacgt cgtcgccagc
      481 ggcgatccga tgttgcacgg tgtcggcggc ttgctgatca ggttgttcgg cgcggaccgg
      541 gtgacggtgt tgccgcacgt gtcgtgtgtg acgctggcgt gcgcccggct gggctgggcg
      601 gttcaggaaa ccgaggtgat cagcctgatg acctcggagc cgcacacggc gatgcggcgc
      661 ggcggacagg ccgtcgttct gtgccgcgac ggcgccgggc cagccgcatt ggcccggcta
      721 ctcaccgacg gcggtcgcgg cgattccgaa ctgacggtgc tcgagcagct cggcggaccg
      781 gccgagcgtc gtcgcgatgc caccgcgcgc gagtgggcgg ccaatccacc cgatgacgtc
      841 gacgatctca acgtggtggc ggtgcggtat ctacccgacg accctcggct atcggtgttg
      901 cccgacgatg ccttcgccca cgacggtcag atcaccaagc agacgatgcg ggccgtcact
      961 ctcgcggccc tggcgccgcg gaagggggag ctgctgtggg acgtcggtgc cggatcgggc
     1021 agcatcgcga tcgagtggtg ccgcaacgga tctcgctgcc gcgcagtggc attcgagcgt
     1081 gacgagcagc gccgcaaaag gatcgccgag aacgcgttgg cgcacgccgc cgtggtggac
     1141 gttcgcggcg aggcgccggc cgcgttcgac gacgccgtgc tgagcgatcg gggcgtcgac
     1201 ggggtgccgc ggccgtcggc gatcttcatc ggcggcggcg tgacccaact cggactgctc
     1261 gaggcctgtt ttgaccggct tccggcaggc gggcggctgg tcgccaatgc ggtcaccgcc
     1321 gaatccgaag cggttctcgc ccagtggtat tcccgcgtcg gcggtgagct tcgacggttc
     1381 cagcactacc gcggcgaacc ggtcggcggc ttcaccggct ggcgacccgc gctgcccgtc
     1441 acgcagtggt cggtgaccaa gcaatgaccg tgtatttcat cggggccgga ccgggcgccg
     1501 ccgatctgat caccgtgcgg ggtcagcggt tgctcagccg ctgccccgtc tgtctctatg
     1561 cgggttcgat catgcccgaa gacctgctgg ctctgtgccc gccggacgcg cgggtgatcg
     1621 acaccggccc gctcaccctc gaccagatca tcagcgagtt ggccgacgcg cacgcggcgg
     1681 gcctcgacgt cgcgcgactg cattctggtg acccgtcgat ctatagcgcg ctggccgaac
     1741 aatgtcgacg gctcgacgcg ctgggcgtcg attacgaaat cgtgcccggc gtaccggcgt
     1801 tcgcggccgc cgcggcggtg ttgggccgtg agctcacggt gcccggcgtc gcgcagacgg
     1861 tcacgttgac ccgggtggcg acgttgtcga ccgcgatgcc gcccggcgag gatttgcaga
     1921 cgctcgcggc gtcgggttct acgctggtgc tgcatcttgc agccgcgcag atcgacgcga
     1981 tggtgccgca actgctggcg ggcggctacg ggccggaaac cccgtgtgcc gtcgtggcat
     2041 tcgcgacgtg gccgcaggag acggtgatcc ggtgcacgct gtccgatctc gccgaggaga
     2101 cgacggccgc aggcgtcacc cgcaccgcgg tcatcattgt tggggatgtg cttgccgccg
     2161 agggcttttc cgacagctac ctgtactcgt caggtcgggt tcggcgcggg cggcattgat
     2221 gcggattctg ctgctgggcg gcacatcgga ggctcgtgcg cttgccgcgg aactacatcc
     2281 ggatgtcgag gtgatcagct cgctggcggg tcgtgtcccg gagcccgcgc tgccggtggg
     2341 cgaggtgcgg atcggcgggt tcggcggcgt cgacgggatg cggcgctggc tgtccgaatc
     2401 cggtgtcgac gccgtcgtcg acgccaccca tccctacgct gcgaccatta cggcccatgc
     2461 cgcggcggtg tgcggcgagc tgggtctctc gcatctggtg ctggcccgaa ctgcgtggga
     2521 tccgggggat gcactcgtcg tgggctccga tgtggaagcg gcgaagaccg ttgccgccga
     2581 cgggttttcg cgggtattcc tgacgacggg gcgctcgggc gtaaaggcgt tcgtcgacgc
     2641 cgacgcgtgg ttcctgatcc gggcggtcac cgcaccggat gccggcacct tgccccgccg
     2701 ccaccagctc gtgttgtcgc gcgggccgta ccactacgag gacgagctgg ccttgctgcg
     2761 cgaccacggc atcgacgccc tggtgacaaa gaacagtggg ggagcgatga cgcggcccaa
     2821 gctggacgcc gctgcagcgc ttggcgttgc ggtggtgatg gtggaccgcc cgccgctgcc
     2881 gcccaacgtc gtcacggtcg ctaccgtcga cgaggcggcc gactgggtca gatcgactgg
     2941 tagctgttga tcaggtcgag cgtttcgagt tcccggatcg cttggcagcc gcggtgcagc
     3001 atcgtgagaa tcatctcgtc gccgcgttcg gtggaagcgg cgaaggcaaa gccgagtccg
     3061 gtgatcaacg gtgcctggat gacgccgaga cggtagtcac gccaacaggt ctcgacgtcg
     3121 tagtcggtca cgccgtaccc caggagcgcc gcgtgatacc gcccgactaa gtctttctcg
     3181 atgtccgctc gcagttcggc ggccaggctg gtcgcggtga aatacgccag atccctggcc
     3241 ggcaggccga tccccatcgt ctgccaatcg acgaccgtga tgcgggtgcg gtcgggatcg
     3301 aacagcatgt tgtccagccg atagtcacca tgcatcagcg cgaaccgctt cggctcggcc
     3361 agcagccacg gtgtcaccga tcccattgcc gcgcgcaacg tttccttgtc gtccgcgccc
     3421 atccgcgcgc caagtttttc gatcgtgatg tcggcagcca tcaccgatac atcgccgaga
     3481 cccttcgctg ctgcctcgtc gcccggcttg ggcatcgaaa tggccgacag cccaagccaa
     3541 gtcggatcac accagctcgg gccgtgtagc ccggccaacg cctcgaccgc cagttgggcc
     3601 tcctggggcg tgcagcctgc gatctgatcg ccctggacgg cgggcgccat atcggcgagc
     3661 agcaaggcga aatccgcccc gctctcgctg atgtcgctgt ggaaacaccg cggcaccgga
     3721 atgcgcaccc ggtccgcgac gtcggtgtag aactcgacct ccgagagata gccgagcgcg
     3781 acgcgttccc ggaccgtgtc gtcttgggcg ggcagcttga tcgcgaatga acgcggcagg
     3841 tcggtatccg tggcataggt cgccgacacc cgatacgtgg cgccggtctg gccggtgccg
     3901 atcggggaga cgtcgacgtg tttcacctcg actgcgagga cagccgacag ccagtcgctg
     3961 gtcagatcgc cggggtgacg cggtatcggc acgatggggg ccacgcgcgg acactaaccc
     4021 gcgtgacaaa aagagtcaaa gatgtcacgt cgggtagtgg cgcggcgtga agactcggtc
     4081 gtcgtaccac tgcgtctgcg acgacccgat gatcagtagg cagcgcatgt cgacatcggc
     4141 ggggtcgagg tcggcgagcc ggaccacctt gacctcttcc cgcggaccgg acacgtcgcg
     4201 gccgatcacc acaggcgtgc tggaatcgcg gtactcgagc agcagatccc gcatcgcgcc
     4261 gacctgccaa gtgcggctct tcgacgccgg gttgtagatc gccagcacca ggtcggcctg
     4321 tgccgcggcc gtcagccgct gctcgatgac ctcccacggc ttgagccgat cggatagcga
     4381 gatgaccgca tagtcgtggc ccagcggggc accgacccgg ctggcgacgg cctgcgcggc
     4441 cgtcattgcc gggatcaccc gcacctcgac gcccggccat tgcttggctt cctccaacac
     4501 ggccgtcgcc atggcgaaca cgccgggatc gccggaggac accaccgcga ccgcgcggcc
     4561 ctgttgcgcc agcgtgcagg ccagccgagc acgcgcaggc tcgtcggtgt tgtcactggg
     4621 atggcggcac tgaccgtcgc gggtcccgac gcggtccagg tacgggccgt agccgatcag
     4681 gtcggtggcg gcggccagct cgcggcggct ctgtcgggtc atccaatcgg tgtcgcccgg
     4741 accgaggccc accaccgcga cgctgccggt ggacgcctgg ggccgtggtg cgcccggcag
     4801 catcgccaag gagaagtacg gcacgctggc gtcgtcgacc tcgccggcag gcagaacccg
     4861 ttccttcggt gtgctggctc gctcaacgta gaacgtctca tccagccgtc cagcggatga
     4921 aagtgcttcc cgcacagcgg gatacgagcg acccaacttg agcacgacgg cggcgtcggt
     4981 gtcggcgagc ctgcgcttga gttcgtcgac gggcagcgtg ccgggcagaa ttgtcagcac
     5041 ctcgtcaccc tggaccaggg gggtgccggt cgccgccgat gccgcgctca ccgacgtcac
     5101 accagggacg atgacggcgt cgaaccgttc ggtcagccgg gtgtgcatgt gcatgtagga
     5161 gctgtagaac agcggatcgc cttccgccag cagcgccacg tggcggcccg catcgagatg
     5221 cgcggcaatc cggtcggcgg actcgcggta gaactcctcc atcgcgccgg catagccgcc
     5281 cggatggtcg gtggtttccg tggtgacggg atagaccagg tgctcttcga tctggcccgg
     5341 ccgaagatac ggttcggcga tcccgcgcgc gatgctgcgg ccgtgccggg cgctgtggta
     5401 ggcgacgacg tcggcctcgg caatcacttt ggccgccttg accgtaacga gctcaggatc
     5461 gccgggcccg agcccgacac cccacagcgt gcccctgctc attcctgttc actcgcaatc
     5521 gcattcacgg ccgcggcggc catcgcgcta ccacctcggc ggccctgcac caccagatac
     5581 gccatgccgc gtggccggtc gatcagttcc tgcttggact gcgccgagcc gacgaaaccg
     5641 acgggtccgc ccaataccgc ggccggggtg ggcgcaccat cgtcgacgag ctcgagtaac
     5701 cggaacaatg cggtcggcgc attgccgatc gccaacacgg cgccaccgag ccggtccgcc
     5761 cacaggtcca ccgcggccgc cgaccgcgtc gtgccggtgc gctcggccag ctcggccgcg
     5821 cgggtgtcgg caaccagcga cacgacctcg ttgtcgccag gcagccgcga tctggtgatg
     5881 cccgctgcca ccatcgacga atcgcacaac accggcgcac cggcggccag tgcggcatgg
     5941 ctcttggcga cgacgtcctc gctgaaggcg acgtgctcgg cgacgtcgac ctgcccgcaa
     6001 gtgtggatca gccggaccac cacgcgcgcg acgtcatcgg ggaaccgcga caggtccgcc
     6061 tcggcgcgga tcgtcgcgaa cgactgccgg tagatctcgg cggcgtcgcg gatgtaggcg
     6121 agcactcgct caccctacgg gggcggtgta cgcagccggt atccgtctcc agttgccacc
     6181 aagacgcctc cggccggtgg gctgccgcac gcccgatcgc agcccacgaa gtgccggtgt
     6241 ccgccggcgg gatcgtcgag cgctgccgcc gcatcggcgc gtacgtcggc agccgaatgt
     6301 tcacagccgg ggctgcccgt gcacgcgctg accgacagcc atggcgagtt tccgtcgaac
     6361 accaatccca ttggtgccaa gacacgcagc gcggtgtcgg cgacaccttc gtcgaggtcg
     6421 aagacgagca ccgagcgcca cggcgtgacg gccatcggcg cgtcgatcgc ggcgaggaac
     6481 tccgccacgc gagcttgcag cacgcccagc ggaaccgcgg cgccaagcgc gacgcggcca
     6541 tcgtcttgcg gaagccagcc gacgggcggc cggacgaccg gtggcgatgt cctccccggc
     6601 gcagcgctgg gagtgaactc ctgcagcagc ggtgccgtgt ccgccaactc ggctatccgc
     6661 catgcctttc cgtgcgtgac ggcgaactgt gtggcgacgg cgacgagagc agccaccaca
     6721 ccagaagtgt tcagcctgac gccggtatcg cgccccgaca gcagcagggc ggccgtcgat
     6781 tcgctgacga agtgtgcgcc aacgtcggca gccagtcccg aaatgtcggc gcgcccgtcg
     6841 tcgacgccga acatgaacct gcccggtagt tccgccaagg cgggctcgga ctgaatcgcg
     6901 tcgtccaatt cggtgaccag tccgcggatg tcggtgcttc cgccgacacg gccggacagc
     6961 ggtgacgcga cgatgttgcg tacgcgctca tgtgtttgcg atggcaacag gcctgctgca
     7021 gcgatgattt cggcgaccgc cgcggtatcg gtgaggcttc gcagttggat gctgccgcgt
     7081 gacgtgagtt ccatggcagg tgacccgaac ctcgtcgcgg tgtgggcaag tgactccaat
     7141 tgagcgggcg agatcacgcc gccgggtaac cgcactcgag ccagcgcgcc gtctgcggcc
     7201 tgatgcagct gcagcgcgcc cgggcagaga tcgcgatcac gggttctggc caccagatca
     7261 ccgtacggcg cgaaaacacg cggggcgacc aagcgcccgc cagcattgct gtgccggccc
     7321 atatgcgaag aaacgagcgc acctcagctt tgccaaatca gcatgagtaa gtactacttg
     7381 ccggaaaaca ggtaggtggt tactcataag gattgctaac tacccgtcgt aggtttctcc
     7441 catccagcag ctgggttcac cggcccggca cgacaagtgg cacggggaga ttgaaatgac
     7501 tgaagtacaa gcaccattcg gcgctccgcc ggacgccgtc gagcagaccg ccgacgttgc
     7561 cgagacgcga acgccggtcc tgatcactga acagcaggtg ttgttcgcca ctgcagcggc
     7621 actgccggtg cggccggcaa aggcccgccg ccgctggact gaaacggtgc gcgggttggc
     7681 agcgagcttg acggcggcct tcgcggcctc cagcaacgaa gcccggccca agcgacagca
     7741 ctacccggcc cgcaatgact tccttgagga ttcacgcatg gcgcgggaga tgttgaggct
     7801 ctgagcagtg agcacaacga gccacgccgt tgctgggatg agcgtcaggc ctgggcggga
     7861 acctgccatt ccgccctggc ttcgctcaat tcccacgtca acgggatgcg gcgagtgctc
     7921 aatgcggcta ctgcgtacaa ccggctgaat taaccagata tacaggtacc cctctacgcg
     7981 tgagatgttc cgtcagcggc gttaattttc tccatgaggg gttccgggtc ccccgggcac
     8041 cagaagaaca cagggtggga gcaactcaaa tgactgacat ggcaacgggg tatcgccccg
     8101 agctcgacaa gaacgacacg tcgccggttg ccccggcggc caacgcattc gagacgcaga
     8161 cgctgatcac cgagcagcag gtgctcttca gcacggctgc ggcggtcgcg ctacctccga
     8221 cgaaaacccg ccggtggagc gacgcggtcc tcgcgatcgc gtcggcggtc ggtagctggg
     8281 tcgcgagcgc agcgaagccg ccaacgaaac cggcttaccc caagcggcat gtttggctcg
     8341 agaacgcact catgtcgcgg gagatggatc ggctgtagtc agctggccag cgcgatgggg
     8401 ctgaccacgt ccgaatactg cgacgcgtca ccgacaacgg cgcggctacg ctggccgtgg
     8461 atatccactg ggatgtctcc ggccagcgtg acgcggttca gccgccggta ctggtcgtcg
     8521 tagtcggcta cggcgtagtg ctgggtggcc cggttgtccc agatcgccag gtcgcccggc
     8581 tgccagctcc agcgaatggt gttctccagc ttggtgactc gagcctgcaa caggttgaac
     8641 aacgtcgccg actctgtcgg gcccagtccg acgaactgtt tgatgaaatg gccgagcagc
     8701 agcacgcggt taccggtttc cgggtgtacc cgcaccaccg gatgttcggt ctcgtagtac
     8761 tcgcgcacga actcttctcg atactcgcgg accgtgtccg ccaattcctc atgcctgtcg
     8821 tcgtactcgc ggacgtagtc gtactgattg gtgtgcacag cccacaggtt ttcggtgagc
     8881 acccgcagcg gcgcaggcaa ctgctcatag gccgcttcgg tcgacgccca cgcggtggtt
     8941 ccgccgtagt tgggcagcga aacagcccgc agcagagacg ctttcgggat gcggtcgacg
     9001 aacgtgacgt cggtgtgcca gctgttggcc ttgtcgtagc gagagtcgat cggcagcacc
     9061 gtctcgccct ccgacagcgt tggatgcgcc gttgtgggag tgccgagctt gcttgcgaac
     9121 gcaagctggc cgtcgtcatc gaggtcgtgc tggtcgcgga agaagatcac cttgtgcttc
     9181 agcagcgcgt cgttgatcgc cgcggcagtc tcgacatcaa ggtcgcccgc caaccggacg
     9241 ccgtctacgc gagcgccgat gttcgcgccc agtttcacca catcgaagta atcggccgtg
     9301 cccgaatcgt gcgacaggtc agcgcgggcg aatacggttt aaattgggcg cgattcgaag
     9361 acggatgtga ccgccgccac accgcggccc ctagtgatcc gtcccacaca agatgatgaa
     9421 taggcgccgg taggacagta cgttctccct aactgcactc gtgtacaaca acgagtcggg
     9481 gaaggaacga cgatgttcgc actcattgcc ttgggcgtgc tcctcctggt cctcatcgcg
     9541 ggcctcggat tgccttaccc gcagacgtgg cacggccgct ggggtagcga cggctaagga
     9601 gaggcctcag ccgaggcttg gacggtacgt cccgaagctc cagacgttgc cgtgcggatc
     9661 gcgggcggcg aactcccgtg agccgtagtc ctggtccgtg aggcccatga cgatatcggc
     9721 gcccgccgcg gcggcgcggt cgtgtagggc gtcggggtcg tcgacgacga cgtaccgcaa
     9781 gaccgggcca ggcaaggcga acgggctcgc atcgtcgcgg tccgcatcgt tgatcatcac
     9841 cagcccgttg ccgactttca cctcggcatg cttgagcggt tggtcggggt ccgggggatg
     9901 cacagcggtc gcgacggcgc cgaaggtctg ctcgagccag cggatcgcgt cgcgagggtt
     9961 ggagtaaggg acgatcggca ccgccccgtt gattgtcagt gtttcgctca tgggcgccac
    10021 gctaggccga gggcgcgacc tcgtcttgga cggaatttac ggtggttgcg cgcaactcgg
    10081 tcggcgtgat gtcggccagt tcgcggacct cgcgggccag atgtgcctgg tcgaagtagc
    10141 cgcaggcggt ggccgcggcg gccaattcga tcgccgggtc cgctgtcagg tgcgcggtca
    10201 gtcgttcgaa gcgcactatc cgcgcgacca gcttcggcgg cagcccgacg gtgtcgcgat
    10261 agcgggcgat cagccgccga tggctccagc ccaattcatc tgcgagatcg ccgatcaccg
    10321 cggtaccgcc ggtggccacg atctggctca gcgaccacgc gacgccagga tcggcgggcg
    10381 cggcctcggc gaggcgggcc ctgaaaacct tgtcgatcaa cgtgaatcgt gtcggccagt
    10441 cgggtgcgtc gcccacccgc tgcacgagat cacgcccgaa ccgacccagc acagcgtcga
    10501 tcggcactga ctgattggcc agctcgctca tgggtagccc gacgagttgt cgggcaccga
    10561 gcggcgtcag gtcgacctgc aggcagcgcg ctgacccgcc gtgcccgacc agcaccggcc
    10621 catcggtcac gccggcgaca aatgatccca accgcaacgg cgtggtgtgc tggcgatggg
    10681 cgaccgacca ccccgcgtcg aggtcgatga tcaccgggac gaacgtgcag ggcagctcgc
    10741 ggaacgtcgt cggcccggcc acctgttcct cgtatccctc atagcgaagc accacgccgc
    10801 gcaacgacgg atgtggtgtg cccgccgcga aggtcgcgct cacctacccc acgctagtcc
    10861 gcaccccgtt tcgtgcgtgc ggtacgaatg gtgggtgcaa gagcccaccg tgctgctgct
    10921 gtcgacgtcc gacaccgacc tgatcaccgc ccgcgcaagc ggggcgcgct atcggtgggc
    10981 gaacccgtcg cggctggtcg acggtgagct tgccgagctg ctggccggcg ccgacatcgc
    11041 cgtcatccgc atccttggcg ggtaccgcgc ctggcaggac ggcatcgaca ccgtggtggc
    11101 aagcggtatg ccgacagtcg tcgtcagcgg cgagcagtcg ccggatgccg acctgatggg
    11161 gcattccacg actccggccg gcgtagcgct gcaagcccat gtctatctcg cccaaggcgg
    11221 cgtaccgaat ctgcgacagc tgcacgcgtt tctctgcgac acgctgttga tgactggctt
    11281 tggtttcgcg ccgccggaga ccacgccgag ttggggtgtc ctccagagag cgtcaacagg
    11341 caatgacggc ccgaccatcg cggtgctcta ctaccgcgcc cagcaccttg cgggcaacac
    11401 cgcctacgtc gaggcgttgt gccacgccat cgaggaagcc ggcggccggc cgctgccggt
    11461 gttctgtgct tcgctgcgca cggccgaacc ggagctgctc gacgtgctgg gcagcgcgga
    11521 cgcgatggtg accaccgtgc tggccgcggg cggtgccacc ccggcggccg ttaccgccgg
    11581 cggcaacgac gacacctgga acgtggccca tcttgccgcg ctggacatcc cgatcctgca
    11641 gggcctgtgc ctgacgagtt cgcgtgcgca gtggcaggac aacgacgacg ggctgtcacc
    11701 cctcgacgtc gccacccaag tcgcggttcc tgaattcgac ggccgcatca tcacggttcc
    11761 gttctcgttc aaggagatcg acgacgaggg gctgatctcc tacgtcgccg atccggaacg
    11821 ctgcgcccgc gtcgccggac tggccgtcaa acacgcccgg ctgcgggcga ttccggcgtc
    11881 ggacaaacgt gtggcggtcg tgttctcggc ataccccacc aagcacgctc gcatcggcaa
    11941 cgctgtcggg ctggacaccc cggccagcgc gatcgcgtta ctgcgcgcca tgggcgacgc
    12001 gggatacgtg atcggcgatg ttccgggcgt cgacgccgag gacggcgacg cgttgatcca
    12061 cgcgctgatc gagcgcggcg gccaggatcc cgactggctg accgacaggc agctgagcgg
    12121 caacccgatc cggttaccgg ccaaggagta tcgcgaatgg ttcgcgacgc tgccacatga
    12181 gctcaccgac gcggtcgtca agcactgggg cccgccgccg ggcgagctgt tcgtcgacag
    12241 aagccgggat cccgacggcg agatcgtcat cgccgccctg caggacggga atctcgtgct
    12301 gatcgtccag ccgccgcgag ggttcggcga gaaccccgtc gccatctacc acgaccctga
    12361 cttgccgccc agccaccact atctggcggt ctaccactgg atcgacgccg gtttcagcgc
    12421 ggacgccgtc gtgcacctcg gcaagcacgg caatctggag tggctgccgg gcaagaccct
    12481 tggcatgtcc tcggcgtgtg gtgccgacgc cgccctgggc aacctgccgc tgatctaccc
    12541 gtttctggtc aacgacccgg gggagggtac gcaggccaag cgccgcgcgc acgccacgct
    12601 ggtcgaccac ctcattccac cgatggcacg cgccgaaacc tacggcgaca tcgcgcgatt
    12661 ggaacaactg ctcgacgagc acgccaccat cgccgcgctc gacccgaaca agttgcccgc
    12721 gatccgtcaa cagatctgga cgctcatgcg cgcagcgaag atggaccacg acctcggatt
    12781 ggacgagcgc cccgaggatg actcgttcga tgacatgctg ctgcatgtcg acggctggct
    12841 gtgcgagatc aaggacgtcc agatccgcga cgggcttcac gttctcggtc aaaagcccac
    12901 ggacagtgtc gaactcgatc tggtgttggc cattctgcgg gcgcggcagt tgttcggtgg
    12961 tgatcagacc gtgccggggc tgcgacaggc gcttggcctg gccgaggacg gcaacgacga
    13021 ccgcgccacc gtcgacaccg ccgaggcccg agctcgtgag ctggtagcgg ccctgcagga
    13081 aaccggttgg gacgcagcgg ccgtcgacac gctgaccgac gatcctgtca tagcgggcat
    13141 actgcggttc gctgccaccg aggtggtgcc gcggctggcc gagacggcgg gggagatcgg
    13201 ccagatcctg cgggcgctcg acggtcggtt catcgcgtcc gggccgtcag gctcaccgct
    13261 gcgcggtctg gtcaacgtgc taccgaccgg ccgcaacttc tactccgtcg accccaaggc
    13321 ggtgccgtcg cggctggcgt gggaaaccgg tgtggcgatg gccgattcgc tgctggatcg
    13381 ttaccgcgcc gactacggcc actggccgca atcggtcggg ctgtcggtgt ggggcacctc
    13441 ggcgatgcgc accgcgggcg acgacatcgc cgaagtgctt gcgctgctgg gcgttcggcc
    13501 gatctgggac gacgcgtcac ggcgagtggt ggacctggag ccgatcccgc tggccgaact
    13561 cggcaggccc cgcatcgacg tcaccgtccg catctccggc ttcttccgcg acgcattccc
    13621 acacgttgtg acgatgctcg acgatgcggt gcacctggtc gctggccttg atgagcccgc
    13681 tgatgacaat tacgtgcgcg cgcatgcaca agccgacctg gccgagcatg gtgacgtaag
    13741 gcgctccacc acaaggattt tcggttcgaa gccgggcacc tacggggccg gattactgca
    13801 actgatcgac agccgcaact ggcgcgacga cgccgacctc gcgcaggtct acaccgcgtg
    13861 gggcggcttc gcatatgggc gggggcttga cggtgccccc gccgccgacg acatgaatcg
    13921 gcaataccga cgaatagcgg tggcagccaa gaacaccgac acccgtgaac acgacatcgc
    13981 cgactccgac gactacttcc aatatcacgg cgggatggtc gccaccgtgc gagcgttgac
    14041 cggccaatcg ccagccgcct acatcggcga caacacccgg cccgacgcgg tgcggacccg
    14101 cacgctgtcg gaagagacga cacgcgtgtt cagggcccgc gtcgtcaacc cgcgctggat
    14161 caatgcgatg cagcggcacg gctacaaggg cgcgttcgaa atggccgcca ccgtcgacta
    14221 cttgttcggc tacgacgcaa ccgccggagt gatggccgac tggatgtacg agcggctgac
    14281 gcaggagtac gtgctcgacg acgagaaccg caagttcatg gcggagtcga acccgtgggc
    14341 gctacacggc atggcggagc ggctgctgga ggcggcaggt cgggggatgt gggcgcaacc
    14401 ggaacaggcc acgctcgacg ggctgcgcgc agttctcctc gaaactgaag gcgaactcga
    14461 aggctaggtt ggtctagtgc cagtcacctt tgccgaagtt gccaagtccg agtacatcct
    14521 gctgaccacg ttcaccaagg acggccggcc gaagccgacg cccatctggg cggcgcccga
    14581 tggcgatcgg ttggtcgtca tcacgcagga gcagtcgtgg aaggtcaagc gcatccgcaa
    14641 cacgccgcgg gtcacgctgg ccgcatgcga tatgcgcggc aatcccaaga gcgaaaccat
    14701 cgacgccgcc gcatcgatcc tggacaagtc ggccaacggc gctacctacg acgccattgg
    14761 caagcggtat gggctgttgg gcaagacgtt caacgttttc tcaaagctgc gcgggggaat
    14821 gaagaacaac gtgaccatcg agctgagggc ggcctgagcc atggcggaca cgttcgccga
    14881 cgtcgctggg tccaagtaca tccggctgac gaccttcacc aaggacggca ggccgaagcc
    14941 gaccgccgta tggggtgcgc cggacggcga caagctgctc gtcatcaccg atgacgggtc
    15001 gtggaagacc aagcggatcc gcaacacgcc gcgcgtgacg atccagcgct ccggcggctt
    15061 gggccgaccc aaaggggagc cggtcgaggc agtggcccgg gtgctgccca aggaccagac
    15121 tcgcaaggtc tacgacgccg tcgtcaagcg ctattggtgg cacgcctggt ggttcatccc
    15181 gcattcgctg gtcagaggtg gcatcgacaa ggtccacatc ggcctggaga tcaagcccgc
    15241 gagctagcca ggtgctcgtt gaagaacgcg aacactcgcc ggtaggcgtc ttcggtcgcc
    15301 gcgtcgttgt agccgaagcc ggtgatccga agtagtggct ggttcggcag cttgttcgcg
    15361 aaaccgtggc ccgcatcggg atagaccttg atatcggcgg cgatgccctt gtggtcgacg
    15421 accttgcgca accgtgcggg cgcgcccacc ccgagtgggt cgcggcggcc gaagctggcc
    15481 acaatcgggc acgcgccgtc cagggtttca ctgaggtggc ggggcagcgg cgtgccgtag
    15541 aacggtgccg acgcgccaaa acccttgggc gacatcacca atgcgaattg gccgcccatg
    15601 cagaacccga ccacgccgac ggagccggtg cactccggca tcgactgcac gtgatcgcgg
    15661 gcggcgagga tgtcatcgac ggcgcggccg cgttggctca gcagctcccg aaagactctg
    15721 gtgatgcatc gcgtcctgcc gccgcgcgaa tacaagttcg gtgtgatggc gacaaatccc
    15781 gccgccgcga tgcgcgccga cgtcgactcg ttgtcggggg catatccgat cgcgtcgtgg
    15841 acaacgacga ctccgggcca gggaccccct ccgtcgggaa cgctcagcag cgcatcgatc
    15901 gggccagccg gggtgtcgat ctggatcgtc ttcacgccgg tcaatctacg gaaccctggt
    15961 gcatcctcca gacgttccac ctacatggcg atgcggctat ttgtccttta cgtggttgtc
    16021 gagctcgcgg tgctcgtcgc cctgacgtcg acggtcggct tcggctggac cgtcctggcg
    16081 ctgctcggca cgttcatcgt cgggctcgcg ctggccgggt cgcaggtcaa gcggcacatt
    16141 cgccggctgc agtccggcct gaccgcggcc aaggtgcagg gagcggcggc cgacagcatg
    16201 ctcgtcgcgc tgggtaccgt gctcgttgtg atccccggtc tggccagctc cgtgctgggt
    16261 gcgctgttgc tgctgccgcc gacccgcgcc gccgcccgcc cgctcgtcac cgcgatggcg
    16321 gcccggcggg ttccgctgat cgccgccgca ggtgcagccg ctcccggctg gggatatgac
    16381 gccgcaggcc gcggcgattt catcgacggc gaggtgatcg acgtcgtcga tgtcaaaccc
    16441 caggccgtgg agcgcaagcc cgaatagagt gacgaccctt cttcttaacg ggcgggtgca
    16501 cagccccgcg atgcccgacg ccacggcgat ggcggtaacc gacggcatcg ttgcgtggct
    16561 cggcagagac gacgtcggcc gcgcccaata tcccgacgcc gagatcgtcg acctcgacgg
    16621 cgcattcgtc gcggcggcgt tcgtcgacag ccacgtccat ctcaccgcca ctggactgac
    16681 gctttccggg ttggacctgc gccacgcgac ctcactgcgg cactgtctcg acctcatcgc
    16741 cggctacgtg cgcgctcatc ccgacgggcc catctgggga cacggctggg acgaatccgg
    16801 ctggcccgcg cacaccgccc ccagcactga cgacctcgac ggcgtcgtcg gcgatcggcc
    16861 ggcttacctg gcccgcgtcg acgtgcactc cgcggcggca tccactgcgc tgcgcaggct
    16921 cgcgccggga ctggccgggg cggcagggtt cgatccgcag cggccattga ccagcgacgc
    16981 ccaccacctg gtgcgcgccg ccgcccgcgg ccggctgacc cccgcgcagc gccacgaagc
    17041 gcggctggcc gccctcgatg ccgccgcagc cctcggcatc gtcgccgtac acgaatgcgc
    17101 gggccccgac atcggcggca tcgacgactg gtccgaggct cgcgacaccg agcacggcgt
    17161 cgacatcgtc ggctactggg gtgaacacgt caccacggcc gaacaggccc gtgagttgat
    17221 cgaccgcacc ggcgcccgcg ggttggccgg cgacctgttc gtcgacggcg cactcggctc
    17281 gcgcacggca tggctgcacc agccgtacca cgacgcgccg cactgccggg gcaatgcgta
    17341 cctcgacgtc gacgcgatca ccgcccatct ccgtgcctgc accgacgccg gcgtcaccgc
    17401 gggcttccac gtcatcggtg atgccgcggt caccgccgtc gtcaccgcac tcgagcgtgt
    17461 cgtcgaccag ttcggtgccc cggcggtggc gcgctgcgga caccggctcg agcacctcga
    17521 gatggtcacc gaggaacaag cggcgcggct cggggcgtgg ggagtgttcg ccagcatgca
    17581 gccgaatttc gatgcgctgt ggggcggcga ggacggcatg tatgtacaac gcctcggctc
    17641 tgaccgaggc atgaagctga acccgtttgc gctgctagca tcccaaggcg tgccaatcgc
    17701 gttcggctcg gacagcccgg tgacgggcct gaatccgtgg gcgaccgtgc gcgctgcgat
    17761 tcggcaccgc actccaggca gtgccgtgtc agcgcgagcc gcgttcgcgg ccgcgacgcg
    17821 cggcgcatgg cgtgctggcg gcgcccgcga cggggtcatc ggaactctcg tccccggcgc
    17881 cccggcgtca tacgcagttt gggaagccga cgagttggaa gtcagcgccc cggccgacgc
    17941 ggtgcagcgg tggtcgaccg accagcgctc ccgcgtgccc gccctgccgc gactcgagga
    18001 cccgttgccg cgctgccggc agacggttca tcggggtgcc gtcattcatg gctaaatcaa
    18061 accgtcgacg cgattggggc cgcgccaccg acgacactga catctttccg cccgtcgacg
    18121 acgacgacga atccgacgac gacgtcgact ccgtcgatga cgaggccggc cctgagcctg
    18181 ccgcgtctga caagtcagcc agaatccccg cttgggcgag gcgattcggt caggcggtgg
    18241 tggaccggct ggcgcagctg ggtgtcacca tcgtcgcagg cgtagcgctg tgtgtgagct
    18301 tcccgccatt cggctggtgg ttcatggcca tcgccgcgtt cggcttgctg gcctgggtgc
    18361 tgacgcgcga atccaccacg ctggccggtg gattcggata cgggttcctc ttcgggctgg
    18421 tgttctatct tccgctcctg ccctggattg gcgggttcgt cggtgcaatc ccgtggatcg
    18481 cgttgtgcat cgtgcaggcg ctgttcccgg cgctgttcgg attggccgct gtcgccgtgc
    18541 gacgcttgtc gggttggccg ttctggttcg ccgcgctgtg ggtggcccag gaatggctca
    18601 agtcctctgt gcccttcggt ggatttcctt ggggggtagt cggtttcagc caaactgacg
    18661 gcccgttgct gtcgatagcg caactcggcg gtgcgccact gctgtcattc gccgtcgtgc
    18721 tgaccggatt cagcttggcc gcaataactt tcgaaatcgt tgagtggtgg cgacacgacg
    18781 acagccaaac cgcggcaccg cccgcggtct tgctgcccgg tgtatgtatc agtgccgtgc
    18841 tgctgaccac cgcgttggcg tggccgcatg tccgacagtc gggcctgggc gccggcgacg
    18901 acccggcgat caccgtggcc gccgtgcagg gcaacgtgcc gcggctcggg ctggagttca
    18961 atgcgcaacg tcgcgccgta ctcgacaacc atgtccgcca aacgattcag cttgccgacg
    19021 atgtgcacgc cggtcgggcg ccacagccga tggtggttat ctggccggag aactcctccg
    19081 atatcgaccc gctggtcaac ccagacgccc gcgacgagat aaacaccgcc gccgcggcga
    19141 tcaacgcgcc gatcctcgtc ggtggcgtcg tcgccgctcc tggctacagt cccgacaacc
    19201 cggtgtcgtc gaactctgtg atcgtctgga atcccgggac cggcccggcc gaccggcacg
    19261 acaagaagat cgtccagccg ttcggcgaat acctgccgtg gcgcagcttc ttcaagcatc
    19321 tgtcgtccta cgccgaccgg gccggttact tcgtgcccgg tagcggcaac ggggtggtca
    19381 acgccgccgg cgtacccatc ggagtgacga cgtgctggga ggtgatcttc gaccgcgccg
    19441 cgcgcgagtc ggtgcaaaac ggcgcccaat tcctgactgt gccgtccaac aacgccacct
    19501 tcaccgaggc gatgagcgag cagcaactcg cgttcgcccg gctgcgcgcc gttgagcacg
    19561 accgatatgt cgtggtcgcg ggtaccaccg gaatcagcgc gattatcgcg cccgacggac
    19621 gcgagctggc ccggaccagc tttttcgaac ccgcctacct cgactcccag gtgcggctaa
    19681 agacgcagct cacgcccgcg acgcggtggg gtccgattgt ccaggggctg ctcgtagccc
    19741 tcggcattgc cgctgtgatc accgcaatac tgcacaatgg gagtttcgtg cgtcggctcg
    19801 gacggcgcgg caaggactaa aggagcccca tgaccaccgg ccagcgcccg agtgagcgca
    19861 ctctggtcat catccccacc ttcaacgagc gggaaaatct gccgttgatc gtcggacgcg
    19921 tgcacgccgc gcggccggac gtacacattt tgatcgtcga tgacaacagc cccgacggca
    19981 ccggcgacct ggccaatgag ctgtcactgg cagacaccga ccgaatccac gtcatgcacc
    20041 gcacgtcgaa gggcggcctc ggcgccgcct atctggcggg attcgcctgg ggtctgaacc
    20101 gcgagtactc ggtgctcgtc gagatggacg ccgacggcag ccacccgccc gaacagctgc
    20161 atcggctctt ggacgccatc gatggcggcg cggatctagt gatcggctcg cgctacgtgc
    20221 ccggcggaga ggtgcgcaac tggccgcgcc gccgcctagt gttgtctcgc accgccaacg
    20281 gctattcgcg gatcctgctc ggcgtcgaca tccacgacat caccgccggc taccgcgcct
    20341 accggcgaga ggtgctcgag aagatcgacc tggccgcggt ggactcgaag ggctactgct
    20401 tccagatcga cctgacctgg cgcaccatca acaacggatt catcgtcgtc gaggtgccga
    20461 tcacgttcac cgaacgcgag ttcggtcaat ccaagatgag cggatcgaac atccgcgaag
    20521 ccctgttcaa ggtcgcggaa tggggtttcc gcgggcgcat ggatcgggcc cggggagtcg
    20581 gcgttacccg ctagcgatca ggagccgcgg cggcgcgtct tgatgatgtc gaggcgctcc
    20641 ttgagcagct cttcgagctc ctcgaccgag cgacgctcca gcagcatgtc ccagtgggtg
    20701 cgcggcggct tcgtcttctt cggctccggc gcatcgccct cgatcagcgt gccctccatg
    20761 ccgttgcggc acagccaggt gtgggggatc tcggcgtcgt cggcaaaggg aacgtcgaac
    20821 tcctcgccgt tatcggtgcg gtaccgggcg acctgacgcg gcgccaagtc gtggttgcgg
    20881 tccgtctcgt agctcacggc tccgaggcga ctgcctctca agacacgatc agccatcgtc
    20941 aacactcctc aagtcacaat tttgtccgga tcatcaacgc agcaaaacgg cgcgcagttc
    21001 ccgactcgac cgttcatgat accgggatct gcgggcccgc cggattacag tctggtccgt
    21061 ggcacgtcgt ctcaagcctc agccctgtcg ctggtgtggt cgtgaagtcg ctgatgccgg
    21121 tctcggtcgg cggcggcaat attgcagaca atcctgccgg cagcgcgcct acgaacagcg
    21181 cgccgtggtc aagggaacct cgctggcacc ggattccgtc gtcctgaccg ccgatgaggc
    21241 ctccgagctc tccgaccgcg tctaccaggt cagatgcgcg gcggaggatg tcgcgatcgc
    21301 cgttgaagag ggagcagaag gaaccgaact gcgccagctg tgcgacgcgc tgatgcaggc
    21361 agcgagggcg gccgacggct ggcgttagcc ggcgacttgt tttcgcggtg aacatcgcgg
    21421 cgcggacacc cgttttacga ttgctaacga caaaacccga gatgggaacg cggtgtccat
    21481 atcacctgca gaaaccggcg tgtggcgctt cgacgccttc gtcctcgaca cgcagcgcta
    21541 cgaattgcgt tccggcgatg aggtgatccg cgtcgagccg caggtgttcg acgtgctcac
    21601 ggagttggtc cgcaattccc accggttcgt caccaaagag gagctgttcg actccgtgtg
    21661 gggtggccgc ttcgtcggcg aggcggcgtt gacgagccgc atcaaggccg ccaggcgagc
    21721 gctcggcgac gacggcgaat cgcagcgcta catcaggacc gtccgcggcc gcggctacca
    21781 gtttgtcggc acgattcatg cagacgcagc ggcagagccc gaggcacctc cggagccggc
    21841 gtcgcctcgc cagcacatcg cgttttgtcg tgccgccgac ggcgtccggc tggcatacgc
    21901 ggtggcgggg gagggtccac cgcttgtccg ggccgccaac tggatgacgc atctgggtta
    21961 cgacgtcgaa agcccggtgt ggaagcactg ggtccgcgat ctatccttga agtatcgatt
    22021 catccgctac gacgaacgtg gctgtggcct atctgactgg gacgcaactg atttcacctt
    22081 tgacgactgg gtggacgacc tcgagtcggt ggtggagacg cttgggctgg agcgcttccc
    22141 gttgctcggc gtgtcacagg gcggtgcggt cgcggttgcc tatgccgccc ggcaccccga
    22201 gcgggtgagt cgcctggtgt tgtgcggcgc atatgcgcgc ggccgcgggg tgcgggcggt
    22261 cggcaacgag gaaaagcgcg ctgccgcatt ggatcttgag ctcgcccggg tcggctgggg
    22321 gcgcgacgat ccggcgttcc gccaggtctt cgccgctcag tttcagcccg acggcacccg
    22381 cgccgactgg gccgcattcg atcagttgca acgtcgcacc acctcgcctg agaacgcggt
    22441 gcgattcctc gaggagttcg gccgcatcga cgtgcgcgat ctggcgcgtg aggtgtcctg
    22501 tccgacgctg atcatgcatt cccgcgacga ccatcgcgtc ccgatgcgat atggcgagga
    22561 actggccgcg ttgattcccg attcccggct ggttgcactg gcgagcaata accacctgct
    22621 gaccgccgcc gagcccgcgt ggccggtgtt cctggcggag gtcgacgcct tcctcgcggc
    22681 tgagtgagcg tttgctccct tgttgcccac agatctccat gcaatctcca cacattcttc
    22741 atgtgcggtc gcgagcggcg attcatgctg gtcacatgag aaaacacttc cgcaccctgc
    22801 tcgtgggtgt cagcgcgctc ctcgccctgg gcaccctatc cgcttgctcc ggcggtaaga
    22861 ccaccgaagc gacgaatacg tccgaggcgg tcaccacacc gtcggcgacc gcacagccgt
    22921 tcccgcagtc ggcgagatac gtcgccgata tgaaggcggc cgacggcaag acgatgacca
    22981 tcggcatctc cgtcgatggc gcgtcgatcg ccgcgtacgc ctgtaacggg accgacgacg
    23041 aagcgtggtt cttcggcaat caggctgacg gcaagatcga catcaagtcc cggttccgcg
    23101 acaccctgaa ggccgagttc accggcaccg atgtcgcggg tgacctgacg atgaacggtg
    23161 tcgcctacac gttcactgcg gcaccggtgt cgggtgtggc cggcatgtac accgccgatt
    23221 tcgaaggtgt gcgcgcatcc tgggtggtgc gtgaagatga atcggcgatc ggcgttcagt
    23281 tcaacaccgg cagcatcagc ggccgcgact tcgagcaggc cgaattgcag caactcaacg
    23341 agcagcagtt ccgcagtggt gttcgcaaca agcgacagat tcagcaggcg gcacagattc
    23401 aattcctgca gaacaagacc gcaaggtcga cgatcaacaa ccaagaggtg acgccgactc
    23461 tggtcaacgg caccttccgc ctcggctgat aactgaatag ccttctcgcc caacggccca
    23521 gcacgcgagc tctacaacgt tcgcgccggc tgggccgttg gcgtttgcat cacgcagaat
    23581 caaaccctta agcggccggc gggcgaacgc gtagtgtcgg cgcaccgtga ccgttcccgc
    23641 cgaaatggcc gtgccgctgg cgtcggctac tgctgccatc aatcgccggc ggacccaccg
    23701 tatgtgggga ctgatccgcc tcctgtcacc ggtggtgctg ctcggcgtat ggcagctggg
    23761 cagcgcgctc ggagtgatcc cgcaagatgt cctgccggcg ccgtcactga ttgtcgaagc
    23821 cggcatcgag ctgatcgaga gcggtcagct ggccgacgcg ttgcaggtgt cgggtgttcg
    23881 agtgatcgag ggcctgttgc tcggcggatt actcggtgtc gcgctgggaa ctgccgtcgg
    23941 actgtcgcgg tggttcgagg cgaccgtcga tccgcccatg cagatgatcc gcgcgctgcc
    24001 gcacctcggc ctgatcccgt tgttcatcct gtggttcggt atcggcgagt tgcccaaggt
    24061 gctgctcgtt gcgctcggtg tgagtttccc gctgtatctg aacaccttct cggcgatccg
    24121 tcaggtcgat ccgaaactgt tcgagaccgc tcaggtgctg ggtttctcgt tttggcagag
    24181 gttccgcacg atcatcgtgc ccagcgccgc gccgcaggtg ctcgtgggcc tgcgccagtc
    24241 attggcgatc gcgtggctga cgctgatcgt cgccgaacag atcaacgccg acaaggggat
    24301 tggcttcctg atcaacaacg cccgcgactt tctccgcatc gacatcatca tcttcggact
    24361 gatcgtctat gcgcttctcg ggatcggcac cgatgccatc gtccgcgggc tggaacgccg
    24421 agcactgagg taccgcacat gaccattgcc tccgaacgcc agaccgtcgt cgcaggcgag
    24481 cttcgccatg tgaacaagtg gtacggcgac catcacgtgc tcaccgacgt gtccgtgcgg
    24541 gtcgcgcgcg gcgaaattgt tgcgttgatc ggccgcagcg gctcgggtaa atcgacagtg
    24601 ctgcgcgtgt tcgccggcct gtcgaccgac cacaccgggg agcgcgtcgt gtccggcgcc
    24661 ccggcgctgg ctttccagga gccgcggctg tttccgtggc gcgacgtccg aaccaacgtg
    24721 gtctatgggc tgacgcgcag ccggctgccc cgagcagagg cacagacccg cgccgatcga
    24781 gcgctggccg acgtcgggct gggagatcgc gccgacgtgt ggccgctgac gctgtccggc
    24841 ggtcaggcgc aacgggtttc gcttgcccgc gcgttggtgg ccgaacccca gctgctgcta
    24901 ctcgacgagc cgttcggcgc gctggacgcg ctgacccgcc tgagcatgcg cggactgttg
    24961 ctcgatctgt ggcgcgagca cgggttcggg gtcttgctgg tgacgcatga cgtggatgag
    25021 gcggtcgcgc tcgccgaccg ggtgcttgtc cttgaggagg gccgggtcat ccacacgctc
    25081 gccatcgacg acccgcgtgg atccgcgcac accgagcaat atcgcgccga gcttctcgac
    25141 aagctcggcg tccagatctg acacacgacg gataggactg tcatgcacaa acttgttgcg
    25201 gtgatcgcac tactgggctt agtgctcacc ggctgcgtat cacgcgagga cagctcgggt
    25261 gctaaggaag caccagcgac tgtcccactg tccgaattgt ccgatctcac actgcaggtc
    25321 ggcgatcaga agggcggaac cgagtcgctg ctgcgcgccg cgggggcgct ggacaatctg
    25381 ccgtacaaga tcgcgttctc gaccttcacg tctggtccac cgcaggtgga ggccgcgaca
    25441 gcgggaaaga tcgacttcgc gatcacgggc aacaccccgc caatcttcgg tgcggcgtcc
    25501 aacgcaaagg tgaaggtggt gtccgcctac gacggcggcg ggaacggcga tcagattctg
    25561 gtgcacgccg attcgccgat ccactcgatc gctgatctac gcggaaggag cgtcgcggtc
    25621 ggcaagggca gctcggcaaa cgggcacatc ctggcccagc tcaagaaggc ggggctgtcg
    25681 ccgtcggatg tgaagctggt gttcctgcaa cccgctgatg cgttgtcggc tttcactcag
    25741 cggcaggcgg atgcgtgggc gatctgggat ccttacaccg cgcaggccgc tgaacaattg
    25801 cccgtgcgca gcatcggtca agcgcgagac gtgaccaatg gcaactggtt cggtatcgca
    25861 tcggatgcgg cgctggcaga tccgaggcgc aacaccgcgc tggccgatct gctggtgcga
    25921 ttcgagaaag cggcgcgttg ggcaaaggac cacccgcagc ggtgggccga acgctatgcc
    25981 gccgccgtcg gactcgacgt gaaggtgtca gaggtcgccc agggccgcag cctgcggttg
    26041 cccaccgaac tcgacgacga catcatcgcc tcggagcaga agatcgcgga cctgttcgca
    26101 gaatccggcc agatcgcttc tcccgcaccc aaattcgctg actgggtgga ccggcgctac
    26161 aacgagacct tgcggcccct gctcatcagc agcaattgag gaaatagaat gcccacttcg
    26221 tctgaatcgc ccgctcagcg ggcaaagttt ttctggttcc tgcccacaaa cggcgatagt
    26281 cgatccatcg tcggcgcctc gcacgcgtcg tcgcaccaca ccgtacccgc gaactaccgc
    26341 gcgccgaacc gacggtattt ggccgaggtc gcccgcgccg ccgaccggct cggctacgaa
    26401 ggcgtgctga cgcctaccgg aacatggtgt gaggacgcct ggttgaccgc gtcggcgctg
    26461 ctggccgaga ccgagcggct gaagttcctt gtcgcgttcc ggccgggtct ggtgccgccg
    26521 acgctggcgg ctcagcagac ggcgacgctg cagcggttct cggagggccg cgtgttgctc
    26581 aacatcgtca gcggaggcga cgacctcgag cagcgccggt tcggcgactg gctcagccac
    26641 gacgagcgtt acgcccgcac gggggagttt ctgcacatcg tgaattcggt gtggcagcag
    26701 gactccgtcg atttcgaggg caagtactac accgtcgccg atgcacgggt gtcggatcct
    26761 ccggatccgt tgccgcagat ctacttcggc ggatcgtcgg ccgctgcgct gccgatcgcc
    26821 gtgcagtacg tcgacgtgta cctcacgtgg ggcgaacccc cgcaggcggc cgcggccaag
    26881 atcgagcagg tgcgcaagct ggccacagag cgtggacgca acgtgcgctt cggcatccgc
    26941 ctgcacacga taagccgcga cacctcggcg gcggcgtggg cggtcgccga cgagctgctg
    27001 gccgagctca catccgagca gatctccaaa gcgactgagc tgcacgccaa gtcggagtcc
    27061 gaaggccagc ggcggatgac cgccttgcat ggcggccggg tggaccagct cgaaatctat
    27121 ccgaacctgt gggctggcgt cgggctggtc cgcggcggtg cgggcaccgc gcttgtcggc
    27181 agccacgagg aagtcgcgaa cctgatctac gagtatcact cgctgggctt cgacgagttc
    27241 atcctgtcgg gctacccaca tttggaagag gcctactggt tcgccgaggg cgtgctgccc
    27301 ttgttgaagc agaaaggcgt cgcttaagtc ccgctggtag gccaagctga ctgtgtggac
    27361 gagtggttcg agcgcagccc gttcatcggg ttcgcgccgt ggatcatcta ttgggtcgtc
    27421 tcagacggac ccagcacctg gatgttcggc gcgatctgcg cggtgatcgc ggcgctgaca
    27481 ctcggggtgt cggccggtcg cggcggaccc cgcatcctcg aagtggtcac catcgtgttc
    27541 ttcgtcgccg tgacagttgg cgggatgatc gtcggggccg acgaccgcga ctggatggac
    27601 acctacgcca ccacgctgtc cagcggcgtg ctggccttga tgtccctggg atcgctggcg
    27661 ttcgtgccct tcaccgcgca gtacgcgcct gaatctgcgc cgcaacagga ttgggagaag
    27721 gcggcgttcc gccgcaccaa ccgggtgctc accctgatgt gggcgctggt gttcgcactc
    27781 atcgcggtcc tggggtacgt ggccgtcacc gcgccggcca ccatcggctg gaccaaggcc
    27841 gtcatccccg tcgtggtgat catcggagcg gtgggcatga cgcagatcta tcccacccgg
    27901 gcacgggaga acgcccggcc agcctgatcc agcgctacta tctacgtatg aatgcagata
    27961 agcaggtctg cggacgccgg ctggctgacg agcaagccga cctggttgtc gaggtgttcc
    28021 ggatgctgtc cgacgcgacc cgcgttcagc tgctgtgggc gctcgccgac cgagagcttt
    28081 cggtcaacga cctcgcggga cgggtcggca agccgggacc gtcggtgtca cagcacctgg
    28141 ccaagctgcg gatggcgcgg ctggtgcgca cccggcgcga gggcactcag gtgttctacc
    28201 ggctcgagaa cgatcacgtt cgccagctcg tcaccgatgc ggtgcacaac gccgaacacg
    28261 ccgccggtgg catgcccgca caccacctcg acgacgcgga attgcggctg ctgcacgagg
    28321 gagcgggctg atgggcatcg cacatagtca cgaccacacc cacagcgtcg acgacgccct
    28381 gcgcgacagc accgccggta tccgtgccgt caagatcagc ttggtggtgc tgggctgcac
    28441 cgccacggcg cagctcgtca tcgtcgcact gtcgggctcg gtcgccctgt tcgcagacac
    28501 cgtgcacaac ttcgccgacg cgttgacggc ggtgccgctg tggatcgcct tctcgatgag
    28561 ccggcggcgc gccacccgcc ggtacacgta cggctttggg cgagttgagg atctggccgg
    28621 gttgttcgtc gtggcggtga tcgcgacgtc cgcggtcgtg gcggctgtgc aggcgatccg
    28681 caggctgatc gatcccgttc cgctgcacca tttggcctgg gtcgcggcgg caggcgtcgt
    28741 cggattcctc ggcaatgaaa tggtggcgct gtaccgcatc caggtcggca agcggatcgg
    28801 atccgctgcg ttgcgagcgg acggaatgca tgcgcgcgca gacggtttga cgtcgctggc
    28861 ggtggtggcg ggcgccgtgg gcgcggcgct gggatttccg gtggccgacc cgatcgttgg
    28921 tctggtcgtc gccggcgcca tcggcatcgt gttgatcttc gcggcgcgcg atgtgttcgg
    28981 caggctgctg gatcgcgtcg atcccgacct ggtcgacaac gccgtcacag tcttggccgc
    29041 gcggcccgga gtgcgcgcgg ttcgacgggt caggatgcgc tgggtgggcc atcagctcga
    29101 agccgacgcc gaactcgacg tcgacccatc actgagcctg gccgacgcgc actccgtcgc
    29161 ccacggcgcc gaacatgaac tggcccatgc cattccgaag ctcggctcgg tgatcgtgca
    29221 cgcctatccg gcgcacgacg gcgtactcag cagctgaaca tctcgccgat ctggggtggg
    29281 gcagtggccg gttgcagaca cccgaagggt tgcacgccca catcgctgaa ccgagctccg
    29341 gactggtgag cgtagttcac acagaccagc ccggcctggt cgctgcggca gcggaagtcg
    29401 ccgaaggtca gcgaatgacc gtatggcagc tccggaccgg aacccgcggt gaaacggccc
    29461 gggtccccgt gcaccgagcc gacccgcacg ctcaagccgt cgtagtcgat ccagccgccc
    29521 ttccactctc cataggcgtc gtcgggtcgc ggtggcgggt tcttcagttc caccaggcag
    29581 gccagcgcgc cgccgctgta cttcgtgtcc gtcatgcagc tggtcttgcc cgacggcgtg
    29641 acgaatgcga cgtcctcgcc gagctctgtc gctgcgccgt cgcgggtcgc ggagtgatat
    29701 tgcgccggat cggagggcgt gcccgcctgg acccaggcga tgacgtcgct catcggcgcg
    29761 ccggggccgg gcgctttggc gggagtcgtc gaggtcggtg gcgtggtggt gggcgcggcg
    29821 cgcgtcgtgc tcggtggtgc ggtcaacgac ggcgacgtcc gctcggcgtc cccgccgacc
    29881 gactgtgagc accccgccac cagcacagac gccgcgatca gggcagcaat ccgcataacg
    29941 gccaaggcta gcgggccgac acgcaatccc tggccaggcg cggcggggag gctctttcga
    30001 taccgtcgaa ggcatgcacg aacatgttgt ccgcgcaaac atcgactcgg gcacggtcga
    30061 gggcttcacc cgcgacggcg tgaaccggtg gcggtcgatt ccgtatgcgc ggccgccagt
    30121 cgggccgctg cgactgcgcg ccc
//
LOCUS       DASRJA010000533         6641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_807357,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000533 DASRJA010000000
VERSION     DASRJA010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807357"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..878
                     /locus_tag="VFC01_23535"
     CDS             39..878
                     /locus_tag="VFC01_23535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZN82622.1"
                     /translation="MAGRVEGKVAFITGAARGQGRSHALRLAQEGADIIAIDVCKPIS
                     RSSEIPPSTPDDLAETADLVKAQNRRIVTAEVDVRDYDALKAAVDSGVEQLGRLDIIV
                     ANAGIGNGGQTLDKTSEHDWKDMIDVNLSGVWHTVKAGVPHLLSGGRGGSIVLTSSVG
                     GLKAYPHTGHYIAAKHGVVGLMRTFAVELGAQNIRVNSVHPTNVNTPMFMNEGTMKLF
                     RPDLENPGPDDMKVAAQFMHVLPVGWVESVDISNAVLFLASDEARFVTGLTLTVDAGS
                     MLK"
     gene            924..2198
                     /locus_tag="VFC01_23540"
     CDS             924..2198
                     /locus_tag="VFC01_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN82623.1"
                     /translation="MTDLASVDYFSDQAVAQDPYDYLDYLRSQGPVFREPHHGVVAVT
                     GYAEVMAAFKDVDAFSAVNAIGGPFPPLPFEPEGDDITELIEAHRHMFPINEHLVVMD
                     PPAHTRARSLLTKLLTPKRLKENEDFMWKLVDHQLDEFIANGNCEYLAEYARPFATLA
                     ITDLLGVPEEDRDEIRHALGAGQPEGGVGALDGEPVGLNPLQYLDDKFSAYLEERRRE
                     PRDDVLTTMATAIYPDGSTPELLEVVRPATFLFAAGQETVTKLLSSAVQVLGERPDLQ
                     QAVRENRKLVGPFIEESLRTQSPTKVDFRLARKTTKLGDVHIPAGTVLMLCLGAANRD
                     PLKFEDPHEFRLDRKNVREHIAFGRGIHTCAGAPLARVEGVITVNRMLDRMREITISE
                     AKHGAAGDRRYSYEPTFLLRGLTQLYIEFTQA"
     gene            2237..2842
                     /locus_tag="VFC01_23545"
     CDS             2237..2842
                     /locus_tag="VFC01_23545"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN82624.1"
                     /translation="MDQSAAALAFAIGVRVKQERQNRRWTLDQLAEAAAVSRRMVVNV
                     EQGAVNPSVGTLLKISDALGIGLPALVEPPRPKPVKVTRRGDGATLWSSKSGGRGVLV
                     AGTTPPNVVELWDWTLGPQDVHKSEAHAPGTRELVHVQEGTVTVEVADQSFALDAGDA
                     VAFPGDVAHSYANPHAKPARFSLAVFEPGVGAGLPSDVGDD"
     gene            2835..3575
                     /locus_tag="VFC01_23550"
     CDS             2835..3575
                     /locus_tag="VFC01_23550"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HZN82625.1"
                     /translation="MTDLGELQAFLAGAQVDAAVFTLRPDYRAMLLAVDGLVPGPSDQ
                     ASDALLGAAETAARQALCELPIEQLPHVAAWREAYRAFGAKPQRTRNSVEALLRRASS
                     GLPRVNRLTDVYNAVSVLHQIPLGGEDLTHYSGAPQLIRAVGTEPFDTVADGVAVIEH
                     PEPGEVVWCDDAGVTCRRWNWRQARRTQLDDHTTTALFILDALDPMSDEALDAATDDL
                     VAHLTQLGPDVQVVRRLIASGTMPFEGD"
     gene            3579..4238
                     /locus_tag="VFC01_23555"
     CDS             3579..4238
                     /locus_tag="VFC01_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HZN82626.1"
                     /translation="MLIHPWDAALDTNEWRDWLASTDRFGMLAVNNLDPAHAPIVLPL
                     HFTVAGDELLIHLARPNPVWPHLEAAAEVRLAVASDYAYIPTYWRAKAGGPDEDGVPT
                     SYYSSVQFVCRPTVVDDPEGKAEILTAQLADFQPEGRHAAVGVDTAPYGRMLSGIRGV
                     RLAVLRVDAKFKYDDANPVDHRQRVIGNLEQRGHGLDAGAAAQQRRRLAAIGDWRRYR
                     P"
     gene            complement(4279..4974)
                     /locus_tag="VFC01_23560"
     CDS             complement(4279..4974)
                     /locus_tag="VFC01_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZN82627.1"
                     /translation="MVEQLRPVTRPRLYEVIVEQLCGYIADNEMTRGDRLPAERDLAA
                     KLGVSRASLSQALVALEVQGVLSVRHGDGAILVRRPTEERAIKALREHADRIPDIIEA
                     REALEVKLAGLAAARRSNEEMAAIDRAIATMQAEIEAGDRGVVGDEMFHEAITTAAHS
                     SLLAKLMHEISGLIKETRIESLSQEHRPRASLEGHRRIAEAVRQQDSEQAAAAMAAHI
                     RLVSDVALLRDAD"
     gene            5130..6491
                     /locus_tag="VFC01_23565"
     CDS             5130..6491
                     /locus_tag="VFC01_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZN82628.1"
                     /translation="MPLELIPILALVAMFVAATLLPINMGTLGFVAAFLVGTVAVGMK
                     TDDIIAGFPSELFLTLVGVTYLFAIAQNNGTVDLMVRGAVRLVRGRVAFIPWVMFGIT
                     GVLTALGALGPAAVAIIAPIALGFAKQYKINALLMGMMVIHGAQAGGFSPISVYGVTV
                     NNIVAKAHLVNSPLALFLGSLFFNAAIGVLLFVFLGGRSLIGRTVHDDHDGPIETSSG
                     LSDGVSGTAPSGTGRTTTLAPTETEPRVTTKPLPTTSVTFDQVLTLIGLGALAVFSLI
                     LDLDIGFVSMTVAAVLALASPKAQKGAVSQISWSTVLLIGGVLTFVGVLQEAGSVEWV
                     GNGVAKLGMPLLVALLLCYVGGIVSAFASSTAILGATIPLAVPLLLAGEVSPIGVVVA
                     LAISSTIVDVSPFSTNGALVLANAQDVDRDKFYKQILKYSALVVAIGPLLAWAALVLP
                     GWL"
BASE COUNT         1181 a         2194 c         2077 g         1189 t
ORIGIN      
        1 taaagtattg atcgatcgat caaacttgtg aggtggaaat ggctgggcgt gtcgaaggca
       61 aggtcgcatt catcactggc gccgcgcgtg ggcagggccg cagccatgcg ttgcgactgg
      121 cgcaggaagg tgccgacatc atcgcgattg acgtctgcaa gccgatcagc cgcagcagcg
      181 agatcccgcc gtcaacgccc gacgacctgg ccgagaccgc cgatctggtc aaggcacaga
      241 atcggcgcat cgtgaccgcc gaggtcgacg tgcgcgacta tgacgcactc aaggcagcgg
      301 tcgacagcgg tgtcgaacag ctcggtcggc tcgacatcat cgtcgccaat gctggtatcg
      361 gcaacggcgg ccaaacactg gacaagacca gtgagcacga ctggaaggac atgatcgacg
      421 tcaacctgtc cggcgtgtgg cacaccgtga aagccggtgt gccccatctg ctctccggcg
      481 gtcgcggcgg ctcgatcgtg ctgacgagct cggttggcgg gctcaaggcc tacccgcaca
      541 cgggccacta catcgccgcc aagcatggtg tggttggcct gatgcggacc ttcgccgtcg
      601 aactcggtgc ccagaacatc cgcgtcaact cggtgcaccc caccaatgtg aacactccga
      661 tgttcatgaa cgagggcacg atgaagttgt tccgccccga tttggagaac cccggccccg
      721 acgacatgaa ggtcgccgcg cagttcatgc acgtgctgcc ggtcggctgg gtcgagtcgg
      781 tcgacatcag caatgccgtg ctgttcctgg cgtccgacga agcccgtttc gtcacgggtc
      841 tgaccttgac ggtcgacgcc ggcagcatgc tgaagtagat cgatcgatca aatccgtagc
      901 tcttgggaag gcccgcaaag tcaatgaccg atctcgcgtc cgtcgactat ttctccgatc
      961 aagccgtcgc gcaggacccg tatgactacc tcgactactt gcgcagtcag ggcccagtct
     1021 tccgggaacc gcatcacggt gtcgtcgccg tcaccggcta tgccgaggtg atggcggcgt
     1081 tcaaggacgt cgacgcgttc tcggcggtga atgcgatcgg agggccgttc cctcccctgc
     1141 cgttcgagcc ggagggcgac gacatcaccg agctgatcga ggcgcaccgc cacatgttcc
     1201 cgatcaacga gcacttggtt gtcatggatc cgccggccca cacgcgcgcg cgatcgctgc
     1261 tgaccaagtt gttgacgccg aagcggctca aggagaacga agacttcatg tggaagctgg
     1321 tggatcacca gctcgacgag ttcatcgcga atggcaattg cgagtacttg gccgaatacg
     1381 caaggccgtt tgccacattg gcgatcaccg acctactcgg cgtacccgag gaggaccgcg
     1441 acgagattcg acacgcactc ggcgccgggc aaccggaggg tggcgttggc gcgctggatg
     1501 gcgaaccagt aggcctcaat ccgctgcagt acctggatga caaattcagt gcctacctag
     1561 aagagcggcg acgcgagccg cgcgacgacg tgctcaccac gatggcgacc gcgatatacc
     1621 ccgatggctc gacgccggaa ctgctcgagg tcgttcgccc cgcgacgttc ttgttcgcgg
     1681 cgggacagga aaccgtcacg aagctgctca gctccgcggt acaggtgctg ggcgagcggc
     1741 cggatcttca gcaggcggtg cgcgaaaacc gcaagctggt gggcccattc atcgaggaat
     1801 cactgcgcac gcagagcccg accaaggtgg acttccgact ggcacgcaag acgaccaagc
     1861 tcggcgacgt tcacattccg gcggggacgg tgctgatgct gtgcctcggc gccgccaacc
     1921 gggatccgct caagttcgag gatccgcacg aatttcgctt ggaccgcaag aacgttcgcg
     1981 agcacatcgc cttcggccgt ggcatccata cgtgcgcggg cgcaccgctc gcgcgcgtgg
     2041 agggtgtcat caccgtcaac cggatgctcg accggatgcg tgaaatcacg atcagcgaag
     2101 ccaagcacgg cgccgccggg gaccgtcgct acagctacga accgaccttc ctgctgcgtg
     2161 ggctcaccca gctgtacatc gaattcacgc aggcctgacg aataatgctg cactttgagt
     2221 gcgctatcat gcactcatgg atcagagcgc cgctgccctc gcgtttgcga tcggcgtccg
     2281 ggtcaagcag gagcggcaga accggcgctg gacgcttgat caactagcgg aagctgccgc
     2341 ggtgagccgc cggatggtgg tcaacgtcga gcagggcgcg gtgaatccca gcgtggggac
     2401 cctgttgaag atcagcgacg cactcggcat cggactgcca gcgttggtcg agccacctcg
     2461 acctaagccg gtcaaggtca cccgccgagg cgacggcgcc accctgtgga gcagtaagtc
     2521 aggtggacgc ggcgtgttgg tagccggcac cacaccacca aatgtcgtcg aactgtggga
     2581 ctggacgctg ggtcctcaag acgttcacaa gagtgaggcc cacgcgccgg gcaccaggga
     2641 actcgtgcat gtccaagagg gcacggtcac ggtcgaggtc gcagaccagt cgttcgccct
     2701 cgatgccggt gatgcggtcg cgttccccgg cgacgtggcg cactcatacg caaatccgca
     2761 cgcgaagcca gcgaggttct ctctcgccgt attcgagccg ggtgttggcg cgggactccc
     2821 gtcggatgtg ggcgatgact gacctcggcg agcttcaggc gttcctcgcc ggcgcccaag
     2881 tagacgctgc ggtgttcacg ctgcggccgg actaccgcgc catgctgctg gccgtcgacg
     2941 ggctggtgcc gggtccgagt gaccaggcaa gtgacgcgct gctcggcgcg gcggaaacag
     3001 cagcgcgcca ggcactttgc gagctgccga tagaacagct cccgcatgtg gcagcgtggc
     3061 gcgaggccta ccgagccttc ggagccaagc cgcaacgcac tcgcaacagc gtcgaggcgc
     3121 tgctacgccg cgcgtcgtcg ggcttgcccc gcgtgaaccg gcttaccgac gtgtacaacg
     3181 ccgtctcggt gctgcaccag attccgctcg gcggcgagga tctgacccac tactccggtg
     3241 cgccgcagtt gatccgggct gtcggcaccg aacccttcga cactgtggcc gatggcgtcg
     3301 cggtgatcga gcaccccgaa cctggcgagg tggtttggtg cgacgacgcg ggagtgactt
     3361 gccggcgctg gaactggcgc caagcacgcc gcacccagct cgacgaccac accaccacgg
     3421 cgctgttcat cctcgacgcg ctcgacccaa tgagcgatga ggcgcttgac gcggccaccg
     3481 acgacctggt tgcccatctg actcagctgg gacccgacgt gcaagtggtt cgccgcctga
     3541 tagccagcgg cacaatgcct ttcgaaggag actgacacat gctcatccac ccgtgggacg
     3601 ccgcgctcga caccaatgag tggcgagact ggctcgcgtc caccgaccga tttgggatgc
     3661 tcgcggtcaa caacctcgac ccggcccacg ccccgattgt gctgccgctg catttcaccg
     3721 tcgcaggcga cgagctgctc atccacctgg cccggccaaa cccggtttgg ccgcacctgg
     3781 aggcggccgc cgaggtacgc ctggccgtcg ccagcgacta cgcctatatc ccgacctatt
     3841 ggcgggccaa ggcgggtggg ccagacgagg acggggtgcc gaccagctac tacagctctg
     3901 tgcaattcgt atgtcggcca acggttgtcg acgaccccga aggcaaggca gagatcctca
     3961 ccgcgcagct cgcggacttc caacccgaag gccgccacgc tgccgtcggc gtcgatacag
     4021 cgccctacgg gcggatgctc tccggcatcc gcggcgtcag gctggcggtg ctgcgggtgg
     4081 acgccaaatt caaatacgac gacgcgaatc ccgtcgatca ccgccaacgc gtcatcggca
     4141 atctcgagca gcgcggccac ggactcgacg ccggcgccgc cgcgcaacag cgacgccgtc
     4201 tcgccgcgat cggtgactgg cggaggtatc gaccgtgaca ccggttgcgc cttcgcgcca
     4261 acgaaacggc gtgaacggtt agtcggcgtc gcgcagcagt gcaacgtcgg agaccaaccg
     4321 gatgtgtgcg gccatcgccg cagccgcctg ctcagagtct tgttgccgca ccgcctcggc
     4381 gatccttcga tgtccctcga gcgaggcccg cggacggtgt tcctgcgaaa gcgattcgat
     4441 gcgggtttcc tttatcagcc cagagatctc gtgcatgagc ttggcaagca aggacgagtg
     4501 cgcggcggtg gtgatcgcct cgtggaacat ctcgtcgccg accacacctc gatcccccgc
     4561 ttcgatctcg gcctgcatcg ttgcgatcgc acggtcgatg gcggccatct cttcatttga
     4621 acgtctcgcg gcggccaacc ccgccaactt cacttcaagc gcttcgcggg cctcgatgat
     4681 gtcgggtatc cgatcggcat gttctcgcag cgccttgatg gcacgctcct ctgtcggtcg
     4741 acgcacgagg atcgcgccgt ccccatgacg caccgacagc acaccctgca cttcgagtgc
     4801 taccagcgcc tgactgagtg acgcgcggct gacgccgagt ttggcggcca agtcacgctc
     4861 tgccggcagg cgatcgccgc gcgtcatctc gttgtccgcg atgtacccgc acagttgctc
     4921 gacgatgact tcgtacagcc gaggccgagt gacgggtcta agttgctcaa ccactgactc
     4981 acctactctc tggtccaacc atactcaccc ggcatgcgtc cctccggtaa ccgtggtcac
     5041 atcgcatgtt gacagtgatg accctcactg ctttactggc taggccaatg ctccactagg
     5101 tcagttggcc taccgcctag gagacctcga tgcccctcga actcattccg atcctggctc
     5161 tcgtcgcgat gttcgtggcc gccaccctgc tgcccatcaa tatgggcacg ctgggcttcg
     5221 tcgccgcatt cctggtcggc acggtcgccg tcgggatgaa gaccgacgac atcatcgccg
     5281 ggtttcccag tgagttgttc ctgacgctcg tcggcgtcac ctacctgttc gcgatcgccc
     5341 agaacaacgg caccgtcgac ctcatggtca gaggcgccgt gcgactcgta cgcgggcggg
     5401 tcgccttcat tccgtgggtg atgttcggca tcaccggcgt cctcaccgcg ctcggggccc
     5461 tcggcccggc cgccgtggcg atcatcgctc cgatcgcact cggctttgcg aagcagtaca
     5521 agatcaacgc cctgctgatg ggcatgatgg tgatccatgg cgcgcaagcc ggcggcttct
     5581 cgccgatcag cgtctacggc gtcactgtca ataacattgt ggccaaggca catctggtga
     5641 acagcccgct ggcgctgttc ctgggcagcc tgttcttcaa cgctgcgatc ggcgtgctgc
     5701 tattcgtctt tctgggcggt cggtcgctca tcggccgtac cgtgcacgac gatcacgacg
     5761 gcccgattga gaccagcagc ggcttgagcg acggcgtaag cggcaccgcg ccgagcggaa
     5821 ccggccgcac cacgactctg gcaccgacgg agaccgagcc acgcgtcacg accaagccgc
     5881 tgccgacgac gtcggtcacc ttcgatcagg tcctcacgct gatcggcctc ggcgcactgg
     5941 ccgtgttctc tctgatcctc gatctggaca tcggcttcgt atcgatgacg gtcgccgcgg
     6001 tgctggcact ggcctcgccc aaggcgcaga agggcgcggt gtcgcaaatc agttggtcga
     6061 cagtgctttt gatcggcggt gtgttgacct tcgtgggcgt gcttcaggag gccggcagcg
     6121 tcgagtgggt cggcaatggt gtcgccaaac tcggcatgcc gctcctcgtc gcgctcctgc
     6181 tgtgctacgt cggcggcatc gtgtcggctt tcgcgtcgtc cacggccatc ctgggcgcca
     6241 ccatcccctt ggccgtgcca ctgctgctcg ccggcgaggt gagtccgatc ggagtggttg
     6301 tcgccctggc tatttcatcg accatcgtcg acgtgagccc tttctcgacg aacggcgccc
     6361 tggtgctcgc caacgcgcaa gacgtcgacc gcgacaagtt ctacaaacag atcctgaagt
     6421 acagcgcgct cgtcgtggcg attggaccac tcctcgcctg ggcggcactc gtgctgccag
     6481 gttggctcta gtcataacgg atgaccttga tcggcggccc gctgtattag cgggccgccg
     6541 atttcgttga tgcgacccag ctcacatcaa gtacctacct gtgagccttg acgcccaggt
     6601 cagaacgtgg tttcctgaat tggctagtcc actagctcac t
//
LOCUS       DASRJA010000534         5992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_936773,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000534 DASRJA010000000
VERSION     DASRJA010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936773"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..748
                     /locus_tag="VFC01_23570"
     CDS             83..748
                     /locus_tag="VFC01_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN82629.1"
                     /translation="MSVELAETGEGATQSNADLAYQIIRERLIMLDIRPSEPINDEGL
                     ARQLGFGRTPVREALKRLERDRLVIAYPRRGTFATTVDITDLAHIQEIRRQLEPVVAA
                     RAASSAPLETRVKLSELADEIAKLDPDDDPRTLLRFDVRVHSAIYKASGNPYLEDVLV
                     CQDAHATRIWCLFLDRLSNVAEHVVEHVSLLRAIIDGDADLAAKLASEHVVGFEQAVR
                     AVL"
     gene            complement(745..1305)
                     /locus_tag="VFC01_23575"
     CDS             complement(745..1305)
                     /locus_tag="VFC01_23575"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82630.1"
                     /translation="MVTLRADAARNRQQLMTAAAELFGERGVDVPLDEIARRAGVSIG
                     TLYNHFPNRGALLDAVLPDWAAELNRLAEAARTDPDPWHGFAAFMDGLFDLQARNPSM
                     NDAIARTPVGPVDVASECGRTGGVLETAVNRARASGVLRPDFGAGDLATMIGAMSKVI
                     AMSNGDDAVWRRHLGFVLDGLRTREA"
     gene            1360..2079
                     /locus_tag="VFC01_23580"
     CDS             1360..2079
                     /locus_tag="VFC01_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN82631.1"
                     /translation="MTSTSVRPALVVLGAGPGLGMSVAQRFGKEGYAVALISRSADRH
                     ADYLQALDAAGVDAAAFTADADDPAQLRAAVNAVRKHFGRIDVGYYGPAATMSLAGDI
                     TTLDAAGAESALKGVLPAVDFASLLIPELTERGEGGLLFAGGLSSVVPMPPLAGLALF
                     AAALRNYAITLNAALAPAGVYAGTITIGGLIERGDIHTAMQQNPAFGELGAGTLNPDE
                     LAETVWRLYSEHTEAEAVVTA"
     gene            complement(2069..2788)
                     /locus_tag="VFC01_23585"
     CDS             complement(2069..2788)
                     /locus_tag="VFC01_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HZN82632.1"
                     /translation="MAELVTPEAPILPWPRILRQNWLNLTFLHWAVEPASIAHLYPPG
                     TEPDVFEGSSFVGVVPFQMSLFGTFLETNVRLCSVDRTGRRGVVFLSLDTNRLAIVAG
                     GRWIFGVPYRWARMTYRQEADRRRYTSTLRWRGAQAASSIEVRVGDSLTCGPLEHYLT
                     ARWGAHVIRAGRTWYVPNEHPVWALRRAQLIDFSDNGFLASVGLGELSMRPPDHVAFS
                     DGVAARFGRPVRSTTPRPVTP"
     gene            complement(2798..3535)
                     /gene="prcA"
                     /locus_tag="VFC01_23590"
     CDS             complement(2798..3535)
                     /gene="prcA"
                     /locus_tag="VFC01_23590"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HZN82633.1"
                     /translation="MSFPYFISPEQAMRERSELARKGIARGRSVVALAYSGGVLFVAE
                     NPSRSLQKVSELYDRVGFAAVGRFNEFDNLRRGGIQFADTRGYAYDRRDVTGRQLANV
                     YAQTLGTIFTEQAKPYEVELCVAEVAHYGETKPPELYRITYDGSIADEPHFVVMGGTT
                     DPITSALNESYSENLGLSDAVKIAVSALKAGSNGGSEPRTLGPATLEVAILDANRPRR
                     AFRRITGSALDAVLPEEDSDAKKSDGD"
     gene            complement(3532..4437)
                     /gene="prcB"
                     /locus_tag="VFC01_23595"
     CDS             complement(3532..4437)
                     /gene="prcB"
                     /locus_tag="VFC01_23595"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HZN82634.1"
                     /translation="MTWPHREQLAFPSPLPGVPSVPVDLSSFSDLLRRQAPELLPVNR
                     SAAASATPGDALPHGTTIVALKFPGGVVMAGDRRSTQGNMIAGRDVQKVYITDDYTAT
                     GIAGTAAIAVEFARLYAVELEHYEKTEGVPLTFRGKVNRLATMVRGNLGAALQGFVAL
                     PLLAAYDLDDPDPQAAGRIVSYDAAGGWNVEEEGYQSVGSGSIFAKSSIKKLYTRVTD
                     ADSALRVAVEALYDAADDDSATGGPDLVRGIYPTAVILAAEGAEDVAESRIAELAREV
                     IESRSRSDTFGPDSAAESHDARGDS"
     gene            complement(4434..4628)
                     /locus_tag="VFC01_23600"
     CDS             complement(4434..4628)
                     /locus_tag="VFC01_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HZN82635.1"
                     /translation="MAQEQTKRGGGGGEDDDLTGSTAAGQERREKLAEETDDLLDEID
                     DVLEENAEDFVRAYVQKGGQ"
     gene            complement(4723..>5992)
                     /gene="dop"
                     /locus_tag="VFC01_23605"
     CDS             complement(4723..>5992)
                     /gene="dop"
                     /locus_tag="VFC01_23605"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HZN82636.1"
                     /translation="DEVGAANMILTNGARLYVDHAHPEYSAPECTDPMDAVIWDKAGE
                     RVMEAAARHVASVPGAAKLQLYKNNVDGKGASYGSHENYLMSRQTPFSAVIAGLTPHL
                     VSRQVITGSGRVGIGPSGDEPGFQLSQRADYIEVEVGLETTLKRGIINTRDEPHADAD
                     KYRRLHVIIGDANLAETSTYLKLGATALVLDLIEEGMDLTDLALARPVHAVHVISRDP
                     SLRATVALADGRELTGLALQRIYLDRVAKLVDSRDPDPRAADIVQTWANVLDLLERDP
                     MECAEILDWPAKLRLLEGFRHRENLNWSAPRLHLVDLQYSDVRLDKGLYNRLVARGSM
                     KRLVSEQQVIDAVDNPPTDTRAYFRGECLRRFGADIAAASWDSVIFDLGGDSLVRIPT
                     LEPLRGSKAHVGALLDSVDTAVELVEQLTT"
BASE COUNT         1036 a         2060 c         1886 g         1010 t
ORIGIN      
        1 ctaactgata tatcagttgt gggccacagt atggtacgtt gatacgcgcg gtcaagagtc
       61 aatgtccgac gggagacgag cgatgagcgt ggagctggcc gagaccggcg agggtgccac
      121 ccagtccaac gccgaccttg cgtatcagat cattcgcgag cggctcatca tgctcgacat
      181 tcgtcccagt gagccgatca acgacgaggg cctggcccgc cagctggggt tcggtcgtac
      241 cccggtgcgc gaggcgctca agcggctcga gcgcgaccga ctggtcatcg cctatccccg
      301 ccgcggcacg ttcgcaacga cggtcgacat caccgatctg gcccacatcc aagagatccg
      361 gcggcaactg gagccagttg tcgccgcccg cgccgcgagc agcgccccgc tggaaacccg
      421 ggtgaagcta tcggagctcg ccgacgagat cgcgaagctt gaccctgacg atgacccgcg
      481 cacgctgctg cgcttcgatg tgcgggtgca ctccgctatc tacaaggcgt ccggcaaccc
      541 gtatctggag gacgtgcttg tctgccagga cgcccacgcc acacgcatct ggtgtctgtt
      601 cctcgaccgg ctgtcgaacg tcgccgagca cgtcgtcgaa catgtcagcc tgctgcgcgc
      661 gatcatcgac ggagatgcag acctggccgc caaactggct tccgaacacg tcgtcggatt
      721 tgaacaagcg gtgcgcgcgg tcctctaagc ctcgcgcgtc cgcaggccgt cgagcacgaa
      781 gccgagatgg cgacgccaca ccgcgtcatc gccgttcgac atcgcgatca ccttcgacat
      841 cgcgccgatc atcgtggcga gatcaccggc gccgaagtcc gggcgaagga caccggatgc
      901 cctggcccgg ttcaccgccg tctccaacac tcccccggta cgtccgcatt ctgaggcgac
      961 gtcgaccgga ccgacgggcg tgcgcgcaat cgcgtcgttc atcgagggat tgcgtgcctg
     1021 gaggtcgaac aggccgtcca tgaacgcggc gaaaccatgc catggatcgg ggtcggtgcg
     1081 ggccgcctcg gccagccggt tgagctccgc tgcccagtcg ggcagcaccg catcgagcag
     1141 cgcgccgcgg ttcgggaagt ggttgtacaa ggtgccgatg ctgacgccgg cgcggcgcgc
     1201 gatctcgtcg agcggcacat ccacgccgcg ctcaccgaac agttcagctg ccgccgtcat
     1261 caattgctga cgattccgcg cagcatccgc ccgcaaagtc accaccaggc cattctagct
     1321 aagttgaggt cgggctcaac ttctggtact aagttgagta tgacctcaac ttctgtgcgt
     1381 cccgctcttg tcgtcctcgg tgccggccct ggcctcggca tgtccgtcgc gcagcggttc
     1441 ggcaaggagg gctacgcggt cgcgttgatc tcgcgcagcg ccgaccgcca tgccgactat
     1501 ctgcaagcgc tcgacgccgc aggtgtcgac gcggcggctt tcaccgcaga tgccgacgac
     1561 cctgcgcaat tgcgcgccgc ggtgaacgcc gtgcggaagc acttcggccg catcgacgtc
     1621 gggtattacg gcccggccgc gacaatgtcg ctcgccggcg acatcaccac gctggatgca
     1681 gccggtgccg agtccgcact caagggtgtc ctgcccgctg tcgatttcgc gtcgctgctg
     1741 attcccgaac tcacggaacg aggcgagggc ggtttgcttt tcgcgggcgg actgagcagc
     1801 gtggttccga tgccacccct tgccggcctg gcgctgttcg ccgcggcgtt gcgcaactat
     1861 gcgatcacgc tcaacgcggc gctcgcacct gccggagtct atgccggcac gatcacgatc
     1921 ggcggactca tcgaacgggg cgacatccac acggcgatgc agcagaaccc tgcctttggc
     1981 gagcttggcg cgggcaccct gaatcccgac gaactcgccg aaaccgtatg gcgcctctac
     2041 tccgagcaca ccgaggcgga ggcggtcgtc acggcgtgac cgggcgtggt gtcgttgacc
     2101 gcaccggacg cccgaaccgt gctgccacgc cgtcactgaa cgccacatga tccggcggcc
     2161 gcatactcaa ctccccaagc ccgacggatg cgaggaaacc gttgtcggag aagtcgatca
     2221 gctgcgcccg gcgcagcgcc catacgggat gttcgttcgg cacataccaa gtgcgaccgg
     2281 cgcggatgac gtgggcaccc caccgagcgg tcagataatg ctcgagcggc ccgcacgtca
     2341 gcgaatcacc cacccgtacc tcgatggagc tcgctgcctg ggcgccccgc cagcgcagcg
     2401 ttgaggtgta gcgacgacgg tcggcctcct gccgataggt cattcgcgcc caccggtacg
     2461 gcactccgaa gatccatcta cctccggcga cgatggcgag ccggttggtg tcgaggctga
     2521 ggaacaccac gccgcggcga ccggttcggt ccaccgaaca cagccggacg tttgtctcca
     2581 agaacgtgcc gaacagcgac atctgaaacg gcacgacgcc aacgaaactg ctgccctcga
     2641 aaacgtcggg ttcggtgccc ggcggataca ggtgggcgat gctggccggc tcgaccgccc
     2701 aatgcaggaa ggtcaggttc agccagttct gccgcaggat tcgcggccag ggcagaatcg
     2761 gcgcttcggg cgtcaccaat tcggccatga ccagtcccta gtcgccgtcc gacttcttcg
     2821 cgtccgaatc ctcttcaggc agaaccgcgt ccaacgccga gccggtgatt cgccggaacg
     2881 cccgacgcgg ccggttcgcg tccaggatcg cgacctccaa cgtcgccggc cccagcgtgc
     2941 gcggctccga accgccgttg ctaccggcct tcagcgcgct caccgcaatc ttgacggcat
     3001 cggatagacc gagattctcc gagtaggact cattcagcgc cgaggtgatc gggtcggtgg
     3061 taccacccat caccacgaag tgtggttcgt cggcgatcga gccgtcgtac gtaatccggt
     3121 acagctcggg cggtttggtt tccccgtagt gagcgacctc ggccacacac agctcgacct
     3181 cgtatggctt ggcctgctcg gtgaagatcg tgccgagcgt ctgcgcgtac acgttggcca
     3241 gctggcgacc cgtcacatca cgacggtcgt atgcatagcc gcgggtgtcg gcgaactgaa
     3301 tgccgccgcg gcgcaggttg tcgaactcgt tgaaccggcc gacggccgca aaaccaacgc
     3361 ggtcgtagag ctcgctgacc ttctgcagcg accgcgacgg attctccgcg acgaacagca
     3421 cgccgccgga gtacgccagc gccaccacgc tgcggccccg ggcgatgcct ttgcgcgcga
     3481 gctccgaacg ctcgcgcatc gcctgctcgg gcgagatgaa atatggaaag ctcacgaatc
     3541 tccccgcgcg tcgtgcgact cagcagcact gtcgggcccg aaggtgtcac tgcgcgaacg
     3601 gctttcgatc acctcgcggg ccagctcggc aatccggctc tccgccacat cctcggcgcc
     3661 ctcagctgcg agaatcaccg cggtcggata gatcccgcgc accaggtccg gtccgcccgt
     3721 cgccgagtcg tcatcggccg cgtcgtacaa cgcttccacc gcaaccctca gcgccgaatc
     3781 cgcatcagtc acacgcgtat acagcttctt gatggaggat ttggcgaaga tcgagccgga
     3841 gcccaccgac tgataaccct cttcctcgac gttccagcca ccggcggcgt cgtacgacac
     3901 gatgcggccc gcggcctgtg gatccggatc gtcgagatca taggccgcaa gcaacgggag
     3961 cgcgacgaaa ccctgcagtg ccgcaccgag attgccgcgc accatcgtcg cgagtcggtt
     4021 gaccttgccg cggaacgtca gcggcacgcc ttcggtcttc tcgtagtgct cgagctcgac
     4081 cgcgtacaac cgggcgaact cgaccgcgat cgccgccgta cccgcgatgc cggtcgctgt
     4141 gtagtcatcg gtgatgtaca ccttctgcac atcgcggccg gcgatcatgt tgccctgagt
     4201 ggaccggcga tcgcccgcca tgacgacacc gccggggaac ttcagcgcaa cgatcgtggt
     4261 gccgtgcggc agcgcgtcac ccggggtggc ggacgccgca gcggaccggt tcacgggcag
     4321 aagttcgggc gcctggcggc gcaataggtc agaaaaggac gacaagtcca cggggacgga
     4381 aggtactcca ggtaggggtg acgggaaggc cagctgttcg cggtgcggcc aggtcactgt
     4441 ccacccttct ggacgtacgc gcgcacgaag tcttcggcgt tctcctcgag aacgtcatcg
     4501 atctcatcga gcaggtcgtc ggtctcctcg gccagcttct cgcgacgctc ttgcccggcg
     4561 gccgtgctgc cggtgaggtc gtcgtcctcg ccgccaccac cgccacgctt ggtttgctcc
     4621 tgagccatcg ctgcctcctg cgtatgtcat cggcgggctc acagccgccc gccggttact
     4681 ccacactacc gttcaacggc cggtttgcct gggatagcgc gcctaggtag tgagctgttc
     4741 caccagttcg acggcggtgt cgacggagtc caacagggct ccgacgtgcg ccttgctgcc
     4801 ccgcagtggc tccagggttg gaatcctgac gagcgaatca ccgccgagat cgaaaatcac
     4861 cgagtcccag ctggctgcgg cgatatcggc accgaaccgg cgcaggcatt cgccacggaa
     4921 gtacgcacgg gtgtccgtcg gcgggttgtc gaccgcgtcg atcacctgct gctcagacac
     4981 caaccgcttc atcgaaccgc gcgccaccag ccggttgtac aggcccttat cgagccgcac
     5041 atcggagtac tgcagatcca ccaggtgcag gcgcggcgcc gaccagttca ggttttcccg
     5101 gtggcggaag ccctccaaca gccgcagctt cgccggccag tccaatatct cggcgcattc
     5161 catcggatca cgctcgagaa ggtcgagcac gttggcccag gtttggacga tgtcggcggc
     5221 tcgtggatcc gggtcacggc tatccaccag cttggccact cggtcgaggt aaatccgttg
     5281 cagcgcaagg cccgtcagct cacggccgtc ggcaagcgcc acagtggcac gcaacgacgg
     5341 gtcgcggctg atcacatgca ctgcatgcac gggccgggcc agcgccagat cggtaaggtc
     5401 catgccctct tcgatcaggt ccagcaccaa cgccgtggcg ccgagcttca gataggtcga
     5461 cgtctccgcc aagttcgcat cgccgatgat cacatgcagc cgccggtact tgtcggcatc
     5521 ggcgtgcggc tcgtcgcggg tgttgatgat gccgcgcttg agcgtggtct ccaggccgac
     5581 ctcgacctcg atgtagtcgg cgcgctggga cagctggaac ccgggctcgt cgccggacgg
     5641 cccgatgccc acccggcccg aaccggtgat cacctggcgc gacaccagat gcggcgtcag
     5701 gcccgcgatc accgccgaga acggcgtctg gcgcgacatc agatagttct cgtgcgaacc
     5761 gtatgacgcg cccttgccgt ccacgttgtt cttgtaaagc tgcagcttgg cggcccccgg
     5821 gacactggcg acgtgccggg cggcggcctc catcacccgt tcgcctgcct tgtcccagat
     5881 caccgcgtcc atcgggtcgg tgcactccgg cgcggaatat tcaggatggg cgtggtcgac
     5941 gtagagccgc gcgccgttgg tgaggatcat gttggcagca ccgacctcgt cg
//
LOCUS       DASRJA010000535         9700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2829110,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000535 DASRJA010000000
VERSION     DASRJA010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2829110"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..727)
                     /locus_tag="VFC01_23610"
     CDS             complement(2..727)
                     /locus_tag="VFC01_23610"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: protein motif:HMM:NF004868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HZN82637.1"
                     /translation="MTLSPGVSDTIRVAGHDLVEDLVGKWGFSDVVHLLMTGGAAPSD
                     GEAAMINAILVTFADHGVTPSSVAARLTLLGAPESFQAAVAAGLCGAGTHYLGTMEET
                     ASMLQREVGAAPDLGPADIARAIVTAARTDRRPIPGLGHPEHKNGDPRSPALLAIAER
                     GGLRSRHTDVLLEIPAALHDAAGVLLPVNAAGITGAILSDMGRPPVFGRGLAIIARAA
                     GLVGQLIDEQQNPTAQRMWDSLR"
     gene            971..1306
                     /locus_tag="VFC01_23615"
     CDS             971..1306
                     /locus_tag="VFC01_23615"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN82638.1"
                     /translation="MSAPQASAPARLASNEPSSASELAGAERVRPQSMTKTLATLHEC
                     GLIRREVDADDGRRLLIFLTDEGRARTRGARTSRAEWLTEVLEQRFIESELMAIDQAL
                     TLLERVVEP"
     gene            complement(1560..2408)
                     /locus_tag="VFC01_23620"
     CDS             complement(1560..2408)
                     /locus_tag="VFC01_23620"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN82639.1"
                     /translation="MNASADGRNPYPGEASASAAPPPTGPLNEPLPLGDASGDLGRPL
                     KISEMLADRLRASILGDGLAPGDLLSSEQELISRYGVARGTVREALRLLESEGLIEIR
                     RGRSGGILVREPDLNQVSRSVGVWLAMRGTTMRQLLDFRLLIEPFAAEAAAMSATPEQ
                     RNWLIRIAEQPAEGGLIHAADLHDSIAVASNNDLCRVTAASLYHGFSWHMTEERLSAS
                     ELEATHTAHLRIVRAIGEANPRKARHAMVRHLEDFGEQLENSGRIDDVIVPRSAWLRR
                     AQIPRP"
     gene            complement(2760..3812)
                     /locus_tag="VFC01_23625"
     CDS             complement(2760..3812)
                     /locus_tag="VFC01_23625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN82640.1"
                     /translation="MQLDLDDDQLSLRNAAREILVHACTPEHVRAVRESAPGARQAQD
                     ELWAQLADLDLLVLGLPEQDGGLGQTDLELAILLEEAGRVALPGPLLETLVVTFVLAE
                     TASTPAHRRWLTELGQGTATATIVPGQGLAAWADRAAVALVTEGDELHLVPAEKLDII
                     SLESVDQVRPLATVQCTPDAETRLTADRRALVRLQDRLSVAASAVLVGISAHLVEATV
                     DYVRSRTQFGRPIGSFQAVKHRLAEAHLAVETARPAVWAAAELIAASDPDVSVAAAVA
                     RSYCAQAAAVVNTHALQCHGGIGFTREYDLQLWLTLGKAIERAYESARSSRARIADSI
                     LGPVNADPRGPAGTPR"
     gene            complement(3803..4975)
                     /locus_tag="VFC01_23630"
     CDS             complement(3803..4975)
                     /locus_tag="VFC01_23630"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN82641.1"
                     /translation="MQLHPDARERAFAEEVRSWLADNLPAQPLDPVDTPEGQQQHREW
                     ERLLHGAGYAGLSWPVEFGGAGLEPAMEAIFNEEYLGAGAPERLNRLALGLAGPSIIA
                     FGTDEQKARWLPRILRCEDVWCQGFSEPEAGSDLASVRTRAVRDGAEYVIDGQKIWTT
                     FGAWADWMFALVRTGDSAAGHKALTFLMIPMHTPGVQVRPLRQLHGADGFAEVFFTGV
                     RVPVSNVLGEEGGGWQVAMATLGFERSGGVAQPMRFDRDVAGLIDLVHGCGLADDPVV
                     RDAVASRYAEALVFRRFFQHMITRLNSGQPTGPIASLGKLFWSEMEVRIYETALDVLG
                     ERAQVSAAPGIPAVGRHVHAKYWYARASGIYAGTTEIQKNIISERLLGLPKEPRCS"
     gene            complement(5295..5963)
                     /locus_tag="VFC01_23635"
     CDS             complement(5295..5963)
                     /locus_tag="VFC01_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN82642.1"
                     /translation="MAAIDISGTVRERAARELRDRILTGALPAGARIDIDAITKEFAT
                     SRTPVREALLELSFEGLVQVAPRSGVTVIGISSQDVRDSFTILGVLTGQAAAWAAERI
                     EPEELAMLRNLAVDVAARSGEDSIGEANWHFHQTIHRAAHSPRLMTQIKHAARVVPSN
                     FLTLFPDHEKQSLSEHEELLDALEDGNAKQARLIAERHVLDAGHSLADWLDQREDSVA
                     VPTS"
     gene            6015..7388
                     /locus_tag="VFC01_23640"
     CDS             6015..7388
                     /locus_tag="VFC01_23640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN82643.1"
                     /translation="MVDRRKDKMALVAFMQAGSTSVYAGSWRHPATEHGYLDASYYAK
                     IGRQLEEGCFDLMFFDDRLAMPGVYGGSVAEAVAYGARPVKLDLGAVLGVLAQATSRI
                     GLGATYSTTYYQPFHVARTFATLDHLSGGRAAWNVVTSVNDSEAQNFGVDAHLGHDER
                     YDRADEFMDVVAGLWDTWEDDAILHDRVSGQYADPAKVHELRHVGKYFSAHGPLTVPR
                     TPQGRPVIIQAGSSGRGREFASRWAELIFTGDPGIEVARSHYADQKQRIADAGRDPAA
                     VRICPMAYAVVGESEAHAKEREELFLNDLVHPMASLTLLSELMNYDFAQHDLDDPVTD
                     ELIASVSGIRGLVQNLRTHIGGDTVTVRDLAGHRATLLQGPRFVGTGEQVADQMADWF
                     ETGACDGFVLAATHLPGAFEDVVRMVVPELQRRGVFRTEYESSTLRGHLGLQRPSNTR
                     VGASANA"
     gene            7414..7467
                     /locus_tag="VFC01_23645"
                     /pseudo
     CDS             7414..7467
                     /locus_tag="VFC01_23645"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7687..7818)
                     /locus_tag="VFC01_23650"
     CDS             complement(7687..7818)
                     /locus_tag="VFC01_23650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82644.1"
                     /translation="MNDGKVGGAPCGSKPERSPFGLVNLYPVMWSITIRTVTARRRR"
     gene            7867..8049
                     /locus_tag="VFC01_23655"
     CDS             7867..8049
                     /locus_tag="VFC01_23655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82645.1"
                     /translation="MALYHRDLHVGPGQLIDVNLTLVRLLEQTLLGHDQFSPMPERGG
                     FPAVPMVVRAPAAEPR"
     gene            8059..9000
                     /locus_tag="VFC01_23660"
     CDS             8059..9000
                     /locus_tag="VFC01_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN82646.1"
                     /translation="MADSDSKRFDGDSWDLASSVGATATAVAARRALASKGPNPLIDD
                     PFAEPLVNAVGVDTFIRMMNGDIEAAKDDPAFAPQRLREGMAARTRFFDSFFLDAAEA
                     GIRQAVILACGLDTRAYRLPWPPGTVLYEVDQPPVIEFKTRTLADLCATPTADRRAVG
                     IDLRHDWPAALHDSGFDATLPTAWSAEGLLSYLPPEAQDRLFDSITALSAAGSRLGTF
                     VSFGASSDVPNTGDRVEERGREVSERWRRLGLHLNWAELVYVGERNDVVAYLADRGWQ
                     STVRSTPELYAENGFECPDQPSTLAFGDIRYVSAALT"
BASE COUNT         1654 a         3127 c         3175 g         1744 t
ORIGIN      
        1 gtcagcgcag ggaatcccac atgcgctggg cggtcgggtt ctgctgctcg tcgatcagct
       61 ggccgaccag tccggcggca cgagcgatga ttgcgagtcc gcgaccgaac accggtgggc
      121 ggcccatgtc ggaaagaatc gcgccggtga tgcccgccgc attgaccggc agcagcaccc
      181 ccgcggcgtc gtgcagcgcc gcggggatct ccagcaggac gtcggtgtgg cggcttcgta
      241 acccgccgcg ctcggcgatg gcgagcagcg cggggctacg gggatcgccg ttcttgtgtt
      301 caggatggcc gagtccgggg atgggccgtc tatcggttcg ggccgcggtc acgatcgccc
      361 tcgcgatgtc ggcgggtccc aggtcgggtg cggcaccgac ttcacgctgc agcatcgacg
      421 cggtttcctc catcgtgccg agatagtggg tgccagcgcc gcacaacccc gccgcgaccg
      481 cggcctggaa agactccgga gcgccgagca gtgttaggcg ggcggcgacc gagctcggtg
      541 tcacgccgtg atcggcgaag gtcacaagta tcgcgttgat cattgcggct tcgccgtcgc
      601 taggtgcggc gcctccggtc atcagcaggt gcacgacgtc gctaaacccc catttgccaa
      661 ccagatcttc gaccaggtcg tggccggcga cgcgaatcgt gtcggacaca ccaggactca
      721 gtgtcatcga gggctcctta aaggctgggc ttgatgtcgg cgcttgactg gtgcgggaac
      781 aagcttcaat atatcagtag gcatctaata tatcatgcga agcgacggca gcactcgatg
      841 gagcccagaa catcgcgctg tgaaatgtga ggacatgccc tcgaactcgt cagcgcttcg
      901 agcgcagcga ttgcggattt ttgcgggtcg actgtggcgc cggttgctag atgcgtcggc
      961 ggttgacggg ttgagcgcac cgcaggcgtc ggccccggct cgactcgcgt cgaacgaacc
     1021 gtcgtcggct agtgagctgg cgggcgcgga gcgggtccgg ccccagtcga tgaccaagac
     1081 gttggcgacg ctgcacgagt gcggcttgat ccgacgcgag gtcgatgcag acgacgggcg
     1141 acggctgttg atcttcttga ccgacgaggg tcgcgcccgc acgcgagggg ctcgcacgag
     1201 tcgtgcggaa tggctgaccg aggtgctcga acagcgattt attgagtccg aactgatggc
     1261 gatcgaccag gcgctgacct tgctggaacg cgtggtcgag ccgtgagcac gcacatgatc
     1321 gagtctgtcg ccgcaggaag gatcggcgat cgagcaatac gtatgagcga ttgagctcag
     1381 acggtgggtt cagcgcaaac tgatcagctc atcgtgctct cgcggccacg ccacgaaggt
     1441 ggtttgtcgt acgactgttt caatacacct gtccggaact accgtcacca gaccgaaccg
     1501 cccggtgtgg acagctctgc tggcgccaag cacagcgtgc ccgagcgttt gtcggatcgt
     1561 tacgggcgcg ggatctgtgc ccgccgcagc cacgcgctgc gggggacgat gacgtcgtcg
     1621 atccggcccg aattttccag ctgctcaccg aagtcctcga ggtggcgcac catcgcatgg
     1681 cgggccttgc gcggattggc ctcgccgatg gcgcgcacga tcctcaggtg tgcggtatgc
     1741 gtcgcctcca gttcggatgc gctgagccgc tcctcggtca tatgccagct gaagccgtgg
     1801 tacaggctgg ccgcggttac ccgacacaga tcgttgttgc ttgccacggc gatggagtcg
     1861 tgtagatcgg ccgcatggat caacccccct tcggccggct gctcggcgat gcggatgagc
     1921 cagttgcgct gttctggagt ggctgacatc gcggcggcct cggcggcgaa cggctcgatg
     1981 agtaggcgga agtccagcag ttggcgcatc gtcgtgcctc gcatcgcgag ccagactcca
     2041 accgaccggc tcacctggtt gaggtctggc tcccggacga ggatcccgcc ggagcgacct
     2101 cgccggattt cgatcagccc ctcggactcg agcagtcgca acgcctcgcg cacggtcccc
     2161 cgcgccacgc cgtaccggct gatgagctcc tgctcggagg agagtaagtc gcctggggcc
     2221 aggccatcgc cgaggatgct cgcacgcagc cggtcagcca acatctcgga gatcttcaac
     2281 ggtctgccga gatcgccgga cgcgtcgcca agaggtagtg gctcatttag agggccggtc
     2341 ggtgggggag cggcggacgc cgaggcctca ccgggatacg ggttgcggcc gtccgcggag
     2401 gcgttcacga ccggtcatca cgtaccgagc agagcacgct accggtggcg cacgtagtac
     2461 gcgaactcaa aggtcgtggc atggccggat ggtccacgcc gcggtattgc tgacgggaat
     2521 gtccacgcga ccaacggctc gttcgcccaa gcgacaccgg cattcgcctc ctcaacgctt
     2581 cgtgtactcg gccgaagcgc gagcgcattg gcggctttac ttctcggcga gtgcgagggt
     2641 cggttgggcc tccatcgggc cgatgccggc gatgccgcag gttgcgcccg tgcacccgag
     2701 cagaaccgaa gtctagcgcc gtcatgatgg cattttgccg gtgaaaccga cccccacgct
     2761 catctcggtg ttcctgcggg tccgcgcgga tctgcgttga ctgggccgag gatcgagtcc
     2821 gctatgcggg cacggctgga acgcgccgat tcgtaggccc gctcaatggc ctttccgagt
     2881 gtgagccaca gttgcaggtc gtactcgcgg gtgaagccga tgccgccgtg gcactgcaag
     2941 gcgtgggtgt tcacgacagc ggccgcctgc gcgcaatagc tgcgtgccac cgccgcggct
     3001 accgacacgt cgggatcgct ggccgcgatg agctcggccg cggcccacac cgccggccgc
     3061 gcggtctcga cggcaaggtg cgcctcggcg agccggtgtt tgacggcttg gaaggagccg
     3121 atcggccggc cgaattgcgt tcggctccgc acgtagtcga ccgtcgcttc aactaggtgt
     3181 gcactgatgc cgaccagcac ggcagacgct gcgaccgaca gccgatcctg cagccgcacg
     3241 agcgcgcgcc ggtcggcggt gagcctggtt tccgcgtcag gggtacattg cacggtcgcc
     3301 agtggccgga cttggtcgac gctctccagc gagatgatgt ccagcttctc ggccggcacc
     3361 aggtgcagct cgtcgccttc ggtgaccagc gcgaccgcag cccgatcagc ccaggccgct
     3421 aacccttggc cgggcacgat ggttgcggtg gcagtccctt ggcccagctc tgtgagccat
     3481 ctgcggtgtg ccggtgtgct tgcggtctcc gcgaggacga acgtcacgac caatgtttcc
     3541 agtagcgggc caggcagcgc gactcggccg gcctcttcga gcaggatcgc cagctcaagg
     3601 tcggtttggc cgagtccgcc gtcctgctcg gggaggccga gaacgagcag atcgagatcc
     3661 gccagttgtg cccacagttc gtcctgggcc tgccgcgcgc ctggcgcgct ctcccggacc
     3721 gcacgaacgt gctccggggt gcacgcgtga accaggattt ctcgggccgc attccgcagc
     3781 gagagctgat cgtcgtcgag atctagctgc accgtggctc cttgggcagt ccgagcaacc
     3841 gctcggagat gatgttcttc tggatctctg tcgtgccggc gtatatcccg gaggcccgcg
     3901 cgtaccagta cttcgcgtgc acatgtcgcc cgactgctgg aatgcccgga gcagcgctca
     3961 cctgcgctct ttcgcccaga acgtcgagtg cggtctcata tatccggacc tccatctctg
     4021 accagaacag cttgccgagg ctggcgattg ggccggtcgg ctgaccgctg ttgagacggg
     4081 tgatcatgtg ctggaagaac cgccgaaaca ccagcgcctc ggcataccgt gacgccacgg
     4141 catccctgac aaccgggtcg tctgcgagcc cgcagccatg cacgagatcg atgaggccgg
     4201 cgacgtcacg gtcgaaccgc atcggctgcg cgacaccgcc gctccgctcg aagcccagtg
     4261 tcgccatcgc gacctgccag ccgccgccct cctcgccgag gacatttgag accgggaccc
     4321 ggacgccggt gaagaacacc tcggcgaaac cgtccgcacc gtgcagctgg cgaagtggcc
     4381 gaacctgcac gccgggagtg tgcatcggga tcatcagaaa ggttagggcc ttatgtccgg
     4441 ccgccgagtc tccggtgcgc accagtgcga acatccagtc cgcccaggcg ccgaaggtag
     4501 tccagatctt ttggccgtcg atgacgtact cggcgccgtc gcggaccgca cgggtccgga
     4561 ccgaggccag gtcgctgccc gcctcgggct cggagaaccc ctggcaccac acgtcttcac
     4621 agcgcagtat ccgcggcagc caccgcgcct tctgctcgtc ggtgccaaag gcgatgatcg
     4681 agggtccggc cagccccaga gcgagccggt tcaggcgctc cggtgcccct gcgccgaggt
     4741 actcctcgtt gaagatcgcc tccatggcgg gctccaggcc ggccccgccg aactcgacgg
     4801 gccaggacaa gcccgcgtag ccggccccgt gcagcagacg ttcccactcg cggtgctgct
     4861 gctgcccctc gggcgtgtcg actggatcga ggggctgcgc cggcaggttg tcggctaacc
     4921 agctgcgcac ctcctcggcg aaggcgcgct cacgcgcatc cggatgcaat tgcacgagtg
     4981 ctccagtgct cgtcggtacg ttcgtctgcc caccggggct cttgccaagc cccggtgggc
     5041 gattctctgg ctcgctcgac ctctgatgcg aactctcaca ctctagcaaa atatcctgat
     5101 aataatagac ctaatttaag gcgatcaggc gtgtccgtga agaagccagc tcgacgcatt
     5161 cgagcacacg tcggcacagt cgacacgccg attccgcagt cggcgtgagc actcactgtt
     5221 gcccctctct cgcggccaag cgggaggcgt cggaaccgcc accaagcatg aaagacgcag
     5281 gctcgaacga acggtcacga agtcggcaca gccacgctgt cttcgcgctg gtcaagccag
     5341 tcggccagtg agtgaccggc atcgagtacg tgtcgctccg cgatcaaccg cgcctgcttg
     5401 gcgtttccgt cctcaagcgc atcaagtaat tcctcgtgct cgctcagtga ctgtttctcg
     5461 tggtcgggga acagggtgag gaagttgctc ggcaccacac gcgcggcatg tttgatctgg
     5521 gtcatcagcc gcggggagtg cgccgcgcga tgaatcgttt ggtggaaatg ccaattggct
     5581 tcaccgatgc tgtcctcgcc tgagcgcgca gcgacgtcca ccgcgaggtt gcgcagcatc
     5641 gcgagttctt ctggttcgat acgctcggcc gcccatgccg ccgcctgccc ggtcagcaca
     5701 ccgagaatgg tgaagctgtc ccggacgtcc tgtgagctga tgcctatcac agtcactcca
     5761 ctacgcgggg cgacttggac caagccttcg aaggacagct ccagcaacgc ctcgcgcacc
     5821 ggggtccggc tggtggcgaa ctccttggtg atggcgtcga tgtcaatccg cgcgcccgcg
     5881 ggcagagcgc ccgtgaggat gcggtctcgc agttcacggg cggcgcgttc ccgcacggtg
     5941 ccggagatgt cgatcgctgc caccagttta aaatatcact tggtatctaa catatcagat
     6001 gttaaattcg gactatggtt gatcgacgaa aagacaagat ggcgctcgtt gcattcatgc
     6061 aggcgggcag tacctcggtg tacgccggtt cgtggcggca tcccgcgacc gaacacggct
     6121 acctcgatgc gtcctattac gcgaaaatcg gccgccagct cgaagaaggc tgtttcgacc
     6181 tgatgttctt cgacgatcgc ctggcgatgc ccggggtgta cggcggctcg gtggcagaag
     6241 cggtggctta cggtgcccgc ccggtcaagc tggacctcgg cgccgtgctg ggggtgctgg
     6301 cgcaggccac gtcgcgcatt gggctgggtg cgacctactc gaccacctat taccagccgt
     6361 tccacgtcgc ccgaacattc gccacgctgg accacctttc cggcggccgg gcggcctgga
     6421 acgtggtcac ctcggtcaac gatagtgagg cgcagaactt cggcgtcgac gctcacctgg
     6481 gtcacgacga acggtacgac cgcgccgacg agttcatgga cgtggtggcc gggttgtggg
     6541 acacctggga ggacgacgcg atcctgcatg accgggtttc gggtcaatat gccgaccctg
     6601 ccaaggtgca cgagctgcga cacgtcggca agtacttctc tgcgcacgga ccgctgaccg
     6661 tgcctcgcac cccgcagggt cggcccgtca tcatccaggc cgggtcttcg gggcgtggac
     6721 gcgaattcgc gtcccgctgg gccgaattga ttttcaccgg ggacccgggg atcgaggtcg
     6781 cgcgcagcca ctacgccgat caaaaacaac gcatcgccga tgccgggcgc gatcccgcgg
     6841 cggtccggat ctgtccgatg gcctacgcgg tggtgggcga gtccgaggcc cacgccaagg
     6901 agcgcgaaga gttgttcctc aacgatctag tgcatcccat ggcgtcgctg acgctgttgt
     6961 cggagttgat gaattacgac ttcgcccagc acgatctgga cgacccggtc accgacgagc
     7021 tcatcgcatc ggtctccggc atccggggac ttgtacaaaa cctgcgcacc cacatcggtg
     7081 gagacaccgt caccgtgcgc gacctagccg gccaccgcgc cacactgctg caggggcccc
     7141 gtttcgtcgg caccggcgag caggtcgccg atcagatggc ggactggttc gaaaccggcg
     7201 cctgcgacgg ctttgtcctg gcggcaaccc atctgcccgg cgcgttcgaa gacgtggtgc
     7261 gcatggtggt gccggaactg cagcgccgcg gagtcttccg cactgaatac gagtcctcga
     7321 cgttgcgggg acaccttggg ctgcaacggc cttcgaacac tcgggtgggt gccagtgcca
     7381 atgcttgacg ggttgctcgt ctgcgacctc gccatgttgg cgcccgcccc gctggtcgcg
     7441 accgagctgg gtgagttcgg cgccaacgtg gttaaggtcg aagaaccacg gcatggcgct
     7501 gcgcgggtgg ggcaacccgt gcgacggcat tgggttgata tggaactcga tagcacgcaa
     7561 caagcgattg atcacctcgc atccgtgctg caccgaaggc cagacgttgg tgcagcgcgt
     7621 agtggagcac gcccacgctg ctatcttcag cacccgccgg cagacatcgt gcaagtaggg
     7681 cttggattac cgccgcctgc gcgcggtcac ggtccggatc gtgatgctcc acatcaccgg
     7741 gtacaggttg accaggccga acggtgagcg ctcgggcttt gacccgcagg gtgcaccgcc
     7801 cactttgccg tcgttcatct tggccgaagg cggcgcgccg ttgaatgtcg cccgtacgcg
     7861 gtgatgatgg cgctctatca ccgcgacctt cacgttggtc cggggcagct gatcgacgtc
     7921 aacctcacgc tggtccgact gttggagcag acgttgttgg gccacgacca attttcgccg
     7981 atgcctgagc ggggcggttt ccctgccgtg ccgatggtcg tgcgcgcacc ggccgccgaa
     8041 ccccgctagg agtaccccat ggcagatagc gacagcaagc gatttgacgg cgacagttgg
     8101 gatctggcgt ccagcgtcgg ggccaccgcc acggcggtgg ccgcccggcg ggcgctggca
     8161 tccaagggcc ccaacccgct catcgacgat cccttcgccg aaccattggt gaacgccgtc
     8221 ggcgtcgaca cgttcatccg gatgatgaac ggcgacatcg aagccgctaa ggacgatccc
     8281 gcattcgctc cgcagcgact ccgtgaaggg atggcggccc gcacccggtt cttcgactcc
     8341 ttcttcctcg acgccgccga agcaggcata cgccaggctg tcatcctggc ctgcggactt
     8401 gacacccgcg cgtaccggct gccgtggccg cccgggactg tcctctacga ggtcgaccag
     8461 ccccctgtga tcgagttcaa gacgcgcacc ctggcggacc tttgcgcaac tccgaccgcc
     8521 gaccgccgcg cagtggggat cgacctgcgc cacgactggc cggccgcctt gcacgacagc
     8581 gggtttgacg ccacgttgcc gacggcctgg agcgctgaag gactactgtc gtatttgccg
     8641 ccggaggcac aggaccgctt gttcgacagc atcaccgcgc ttagcgctgc cggaagcaga
     8701 ctgggcacct ttgtgtcctt cggtgcgtcc agcgacgtgc caaacaccgg cgatcgcgtt
     8761 gaagagcgtg gtcgtgaggt cagtgagcgc tggcgtcgcc tcggattgca tctcaactgg
     8821 gccgaattgg tctacgtcgg cgagcgcaac gatgtggtgg cctacttggc ggatcgcggc
     8881 tggcagtcga cggtacgcag cacaccagag ctctacgccg agaatggttt cgagtgtcct
     8941 gaccagccgt ccacgctcgc gttcggcgat atcagatacg tgtcggcagc cttgacctag
     9001 cctgaatttt tggtgcgact ttgtcgggat ggtgtcgaca ggcgaaattt tcctgtcctg
     9061 caatgcagga tcgacggggc cgaggatcga aaccggcgac ctgcagcgaa tgtgaagcca
     9121 cccgaaagta ctagcgcatc aagctccccg atgagctgat cgagttcacg ctcgataacg
     9181 tcagcaacga aggttttcgg cattacgacc gactgcttcc tgcagatgtg ccgagcgttc
     9241 gtccgcgcgc gatgccggcg acctcgcgac cggtcgtcag atcgagattg ccgagaacgc
     9301 caacgcgttc ctatccgctt gggcaaccgt cggtctcatc tcgttgatcg acgaagccac
     9361 cggctaccag tacatgcggg ccgaggacgc gttgcgggtg aagttgcgtg catatctcga
     9421 agaggaaatg cggctgtggg agaagacttt cccggacgag ctgtggatcg agtttggttt
     9481 cctcactgtg ggagggctcg gttcagcagc gtccgatgga ctgggggaag ctagtgaacg
     9541 agctcgtcta tcagtatctc gatgccgatg tctttgactg gcttaagaac aacgcgtcgc
     9601 cttcccgtca cggccagaac tatcaccagc cagttcgaca ctgggaagcc aagattcacg
     9661 ccccaatact ggcccagggc ttccaacgca ctcggtcggc
//
LOCUS       DASRJA010000536        20870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1841155,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000536 DASRJA010000000
VERSION     DASRJA010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20870
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841155"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VFC01_23665"
     CDS             complement(<1..1168)
                     /locus_tag="VFC01_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN82647.1"
                     /translation="MSDAALSMRGPVHGTKDAKKVAIGSGVGAVIETYDFIGFGTAAA
                     LYFGHAFFPGASPLAGTLASFATLGVGFAARPLGGIIGGHLGDKLGRKPVLVASLIVM
                     GLATFVIGLLPTYAAIGILAPILLVTVRIIQGIAFGAEWGGAILMSYEHAPWRQKGRY
                     TGMVQAGFPVGLLLANLVFLVSVHLPGDWAWRLPFLASIILVIVGLIIRAKVPESPVF
                     EDVKEHGDIVKSPIVKVIKEDWRNILRGIGLRIAETAGYAVSITYMISYLKNAELATK
                     SETLTALCIASAIGIVATMAWARLTDKIGRRPVYMWICAFGIPFGVLMFLTVNTGLQL
                     LIIATIVVAYGVCQNALAGAQGAWFPELFTANTRSSGASLAYQISAMVSGFTPFI"
     gene            1367..2191
                     /locus_tag="VFC01_23670"
     CDS             1367..2191
                     /locus_tag="VFC01_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZN82648.1"
                     /translation="MIHSRLGCSTISFRHLSLEPALGWIGALGFAEVDLGALPGVCDH
                     VPYVLDRDAVDAVADTVRASGIRVRSINGDIGDLNRPLDTDGRASRDGHLEMLLALTA
                     ATGAHALVLPCGALDHQPLGSLDDDLDLVAQELTRSAEQAAGHGVEIWTESLHFHRLC
                     WNLERAQKLTDRLAGTPVQVVMDFSHIVASGGDPGEFVDRFAGRIAHVHIRDAVPGNI
                     NLSVGRGDVDFGRGLKALADAGYTGHFALELETRDITDSQRPAATAKTAHLISDLI"
     gene            2204..2977
                     /locus_tag="VFC01_23675"
     CDS             2204..2977
                     /locus_tag="VFC01_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007296234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN82649.1"
                     /translation="MSETKRTAVVTGATSDRGIGMAVAQRYAREGWAVVILDLDGEKS
                     AKVAAEIGNQFAVPAFGYAIDVTSEESVAAAEHAVAEEVRSGALPPLGALANIAGITS
                     PVPFLETTLDLWNTVMAVNATGTYLVTKAFLPAMIDNGWGRIVNMSSVSAQRGGGVFG
                     KVPYSSAKAAILGFTKALAREIADTGVTVNAVTPGAVDTNIRVGSTDDQEAKLARDIP
                     VGRTATTEEIAAVITFLSSDDASYLTGTTVDINGGSHIH"
     gene            2989..4728
                     /locus_tag="VFC01_23680"
     CDS             2989..4728
                     /locus_tag="VFC01_23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019667192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase family protein"
                     /protein_id="HZN82650.1"
                     /translation="MTRLYNDPATFTEDMLDGFLDANARYVVGVEGGVVRATTTPPGK
                     VAVVVGGGSGHYPAFCGVVGQGFADGAVVGNIFTSPSAREAASVARAAHGDAGVLLIT
                     GNYAGDVMNFGLATEQLRSEAIDARYLVVTDDIASAAPDDITRRRGIAGDFTVFRCAS
                     AAAEEGAALDDVERVARKTNDATRTLGVAFDGCTLPGADRPLFTVAQGTMGLGLGIHG
                     EPGVSSHDMPTAADLAALLVDGVLAETPAGATDRVAVILNGLGRIKYEELFVVWKTVA
                     ARLAAAGLTVVLPEVGELVTSLDMAGCSLTVTWLDEELERLWSAPADTPAYRKGQITG
                     AAGTLRERSHTPTRNSATTPTFMDADESSRVGGENVAQALRKIALRMVEAESELGRID
                     AVAGDGDHGRGMVRGTEAASAAADSTANRGGGIGSVLAAAGEAWAAKAGGTSGVLWGA
                     ALSAAARKLGDQGTPTDADVASALRAGHDALVELGGARRGDKTMLDALGPFVESVESA
                     VGCGIDWRNAWLASVDVAGKSAAGTADLRPRVGRARPLAERSLGTPDAGAISLVMCID
                     TVAGFFQHEGASR"
     gene            4725..5180
                     /locus_tag="VFC01_23685"
     CDS             4725..5180
                     /locus_tag="VFC01_23685"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HZN82651.1"
                     /translation="MSLRIVVGSDDAGLQYKQILHRDLETDSRVASVVDVGVGPDEHT
                     AYPHVAVTAARLVADGEADRALLVCGTGLGVAISANKVPGIRAVTAHDSFSVERAILS
                     NDAQVLCFGQRVIGIELARRLVREWLSYRFDPESASAAKVAAICEYEPA"
     gene            5209..6483
                     /locus_tag="VFC01_23690"
     CDS             5209..6483
                     /locus_tag="VFC01_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HZN82652.1"
                     /translation="MIPLTWKHVETQPEDDFVVAPDERLSWARTIGLGAQHVVAMFGA
                     TFLVPVLTGFPPATTLLFSGIGTVLFLLITGNRLPSYLGSSFSVIAPVTAAVASHGTG
                     SALGGLVAVGVLLIIIGVVVHLVGTHWIDVTLPPVVTGAIVALIGFNLAPAAKSNFEK
                     GPLVGLVTLVLLVATLAFFRGIIGRLAIFLAVVAGYVLALILGEVDTSAIKAAPWVGL
                     PEFQTPTFTVAVLPMFLPAVIALVAENIGHVKSVGQMTDTDVDPVMGHALAADGVATT
                     LAGFGGGSATTTYAENIGVMAATRVYSTAAYWIAAAVAIALSLCPKVGAAISAIPPGV
                     LGGATVVLYGLVGVLGVRIWLTNDVDFSKPLNQMTAAIPLIIGIADFTWQAGDLTFTG
                     IALGSIAALLVYHGMRLLGFRKAREPIPQRST"
     gene            6502..8283
                     /locus_tag="VFC01_23695"
     CDS             6502..8283
                     /locus_tag="VFC01_23695"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN82653.1"
                     /translation="MRRTWTWTFDLPPDRLWPVLADTDRFNEAMGLPPYTLEQTPQPN
                     GTVLRRGRGKTAGFNLEWEEKPYEWIAGRHFRQSRVFTKGPFRRFGPVFDLEPDGKGG
                     SHVTYALEWEPLSLVGRAFGARLAAQAGEAVGKRVVQAVAFAQGEQAGERLTPFDLPP
                     PDLPPGARERAFALAREIDNSPYGNGLGTPLAHIVLNGMATDLTHLKPKVLAKKLGVP
                     PRKAIEACLAGVKAGLLTMKWDLLCPNCRGAKLRVAVLSELPRGAHCPSCNIDYDRDF
                     EKNVELSFAPAPAVRPLHDGGFCLSGPMGTPHVAVQVLLSPGERRNVSVELPPGSYRL
                     RTLHPGVFVDIDYESGPFPGLRVTASGVESLNATDQSNEAGSVTFVNDAEFELAALIE
                     DRTWARDALMAPEVISLQAFRDLFAEATLRPGDDAGVSQVALLFTDLRGSTALYERVG
                     DATAYNMVRKHFALLAALVRDHDGAVVKTIGDAVMASFSDPAQAVRAALAMQGEIGST
                     DLVLKVGVHAGPSVVVTLNDRLDYFGSTVNMAARLQGQSIGGDIVLSHAVADDPAVRE
                     EIAAIPQRQETVALKGFTEPVGFVRLL"
     gene            complement(8353..9516)
                     /locus_tag="VFC01_23700"
     CDS             complement(8353..9516)
                     /locus_tag="VFC01_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN82654.1"
                     /translation="MYGLTEQDRRIRDTARAFVETLMPHEVEAELAGGILPKELTAEH
                     QARAIELGLYATNMPTSVGGPGCTALQQVLVQEQCGRVTNGLGWVMATPPQWWVDVAS
                     DYQRERWLLPSVRGEKHEAYAITEEFAGSDVSALQTTAHRDGDEYVIDGVKWHVTSFN
                     LADYVFVEAVLTGGDHVLLVVDLPWPGVEVVRTPQYSHHIADEHPIVAFKHVRVPVAN
                     LVVTEGVPMTFTQDWFRFERMMVAARCVGAAQRLVDEATAFAQNRIVDGRPLGEHQLV
                     AGMLADCATELFAARTMLYEVARSIDAGHDRKTLHGQASMAKLYCSEMAGRVADRAVQ
                     IFGGRGYMRENVAERMFRELRVERIWEGASEIQRIIVGRQLMTRGPGALLDPA"
     gene            9570..10769
                     /locus_tag="VFC01_23705"
     CDS             9570..10769
                     /locus_tag="VFC01_23705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN82655.1"
                     /translation="MTSSRSAEVVVVGGGTVGAWTAVLLAERGVANVVLIEARTLGDG
                     ASSRAAGMVRAQGGTETAIRLGLRSQEFYAASGDRFPLGCGFVAQGYLMPCFTDAEVA
                     QAHERIALQRSLGLDVEWLSSADIDDRQTGLAPGVTLGASYAPGDGYIDAPRNVLAYT
                     AALTAVGADVRERCTFTGLRTADGRVVGVDTSDGPIDTEHVVLTGGPQLAEVGQTAGG
                     RIPAGGTRHQVVVTAPLPLDVNQLPMVFDITSGIYWRPGESGGLLWGMSNPDEPPGVA
                     IDFDTVYYRKARDRIEELFPPVRGLGLRRAWAATIDYTPDHLPILGPLLTDDGPVEGT
                     VVASPAGHGMMWGPAVAQVAADLTATGRCDWLDLTDLGLDRFDAEGNSRLAPEPISLP
                     FPESVAR"
     gene            complement(10813..12849)
                     /locus_tag="VFC01_23710"
     CDS             complement(10813..12849)
                     /locus_tag="VFC01_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZN82656.1"
                     /translation="MTEHRRATVAAMLGARSMAVVGASARPDSFGARMVLEAQRGSAR
                     VHLVNPRYDRIGELACAPSLADLDEPVDVVLLGVPDSALVEQLELAAAVGARSAVLFG
                     TAHGLREKIATTAAEAGMALCGAGCMGFVNNALGVRALGYLEPDPLPSGGIALVTHSG
                     SAFSTLLRARRGFGFRLAVSSGQELVTDTADYVDYALDDPDTRIISLLMETPRSVPRL
                     RQSLLRAAEQDVPVVVLAVGGSPRGRAMVAAHSGALAGEHAAWQALCDATGAMRVSDL
                     AEFTDTVELFSAGRRSNGGGVATVHDSGAERALLADLAHELTAPFADLADETLATIGG
                     MLDDGLAPTNPLDVWGTGASTRNLFGACVQAMSDDPGVGVTALAVDLVTEFDGDTAYA
                     DAVLDVARSTDAPLAVLASVPSAIDAPTAQRLRDNGVPVLEGFRSGLAALGHLVRWPL
                     PVERPGQPARSGRRIPEDAFELLASYGIPVVQSRTAHSIGEALTAADELGYPVALKTL
                     GALHKSDVGGVVLNLADEAVLSVAYTAMAGSLGPAVTVQPMVADGVEVSVGFVRDDAF
                     GPLVVVAAGGTLVELLGDRVVACPPVSDAAARRLLERLRVAPLLRGWRGQSGVDVDGL
                     ADVVVRFSQMAVDLGELFDAVEANPVVASPGGVVAVDALVVPRAGPTRQLGNAK"
     gene            complement(12846..14474)
                     /locus_tag="VFC01_23715"
     CDS             complement(12846..14474)
                     /locus_tag="VFC01_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN82657.1"
                     /translation="MSNTYDALVIGGGHNGLVSAAYLARSGARTLVLEGRGSLGGAAT
                     TESPWEDAPHLRVTRLSYVMSLMPPTIVRELELERHGYKVHPMGPYYQAFPEGGSLTI
                     YEDDPARTYEQLAKWSKKDAEAWPKWNAWLEGIAEVMGPLLTQVPPNIGSRRPSDLLD
                     LARLAWRQRGITVRTTADVTRLLTMSIADLLDDWFESPQIKGALAVNGVIGTWAGPYE
                     PGTAYVMAHHSIGDVGDGQLGSWGYPEGGMGAVSEAIARSARGFGAEIRTNARVARLL
                     VRGGRVHGAVLENGDEIHAPLVITTLHPKTAFLEHIPRTELPNEFVTDIEHWKTRSGV
                     VKINLALAELPNFTADPSRGMAEHHTGSVEMAPTMEYIEAAFQDARAGRPALLPFSDG
                     VIPTTLDKTLNPDGTHIMSLFTQWVPADWSQAPHTEELDAYADRLIDLYDQVAPGFKS
                     SITHLDIVGPYEMERDYGLIGGNIFHGELSLEQLFHMRPAPGYADYRTPIAGLYNGSS
                     GTHAGGGVCGIPGWQAARAALADQKRGLRRLFRG"
     gene            14571..15221
                     /locus_tag="VFC01_23720"
     CDS             14571..15221
                     /locus_tag="VFC01_23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZN82658.1"
                     /translation="MADPAPATEQNEARRIEMLRAAAELICERGFGDTRIADVAKRAG
                     VSSALVIYYFGTRDRLLVDALRYSEESFYEAAEQMLAEVPSLRERLSLLIQWTCVPEG
                     AGEIPGAWGLWFDLWAQAFRHDEIKAGRAELDARWRKMIIDALDSSDLSSKDKRMFAL
                     EFAALLDGLSIQVALEDPLVDSDLAYDIAMHFAERELNLPPAKKRLNGKAKAAKRD"
     gene            complement(15208..16356)
                     /locus_tag="VFC01_23725"
     CDS             complement(15208..16356)
                     /locus_tag="VFC01_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN82659.1"
                     /translation="MTGGAIRLQELAKSFDGVPAVTGIDLDIPAGQFYSLLGPSGCGK
                     TTTLRMIAGFEKPDSGRIELDGRDVVSDPPHKRPVNTVFQTYALFPFMTVWDNVAFGL
                     KYHKISRDDAKRRVGEALELVRMSDFSKRRPAQLSGGQQQRVALARALVLRPRVLLLD
                     EPLGALDAKLRKQLQLELRAVQREVGITFVYVTHDQEEALTMSDQIAVLAEGRVEQVG
                     PPQEIYSAPATTFVAGFMGAANIFDADVLESGDGSAVCSALDTRLGAAVDASAVSGAA
                     AIVIRPERISLQNPGEPIGPGRNVIEGVVAQVVYLGNCTQVHVDVGAPTALVVEVANH
                     AGPTSVTHEPGAKVNCVCTHDAVRVLHRSTAVPIADPVAEQVGALSPS"
     gene            complement(16373..17773)
                     /locus_tag="VFC01_23730"
     CDS             complement(16373..17773)
                     /locus_tag="VFC01_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZN82660.1"
                     /translation="MSSSVVQSQETDIVDQLIAGQEQVFLRRQPRSTELIAQAREHLA
                     GGATSNWQIAEPQAVWMSHGNGSKVYDVDGNEYVDMHGGYGASIAGHAHPAIVAAVSD
                     RVRRGTHFAQPTEDAIWIAGELSRRFGLPLWRFANSGTEATMDAVHLARALTGRDLII
                     KVEGCYHGHHDSVQVSVLPDADEIGPRDHPIGVPGNSGIASAIRDLVIVVPFNDPDAV
                     ARALAEHRGQVAAMILEPVMMNAGIIPPDDGYLAAIRDLVHAEGALLIFDEVKTGFTT
                     GPGGVTALTGVVPDMVCLAKALGGGIAVAAIGGTSTVMSAIADGRYEQVGTFNGNPLS
                     MAATRATLTDVLTPQAYTHLEALANRLRTSLEAVIAQHGFGWQVVTVGAKGCVTFRRD
                     PVREFRDFLQIDARLGHLHWLMQHNGGVFLPPWGKVEQWLLSVQHDEDDVDRFATNFA
                     RFSATLSRFGPGAVAP"
     gene            17868..19097
                     /locus_tag="VFC01_23735"
     CDS             17868..19097
                     /locus_tag="VFC01_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZN82661.1"
                     /translation="MPQPTSRRQFLARAAILAASAPTLGAFLQACSKNAPSSSQPTMT
                     IAAPDNPVTWPIADDNKPIADGLAPEKGATLKIYNYADYLSPQAIKAFEDQYAIKIEV
                     STFNDGDEAITKLRSGVDFDIYNANYTEISRLVNGGLLRPLNHSYIPNIKNVWPGFTN
                     PWYDQEWRYTVPYTIYTTGIGWRSDQVPADIGALKNPYDSLWDPQYKGKTAVLDDFHS
                     AMAMVLLRQGITDVNTSSEADLKMVGEQLNALVKATSPKVTITMYNDLPAGQLGLAQM
                     WSGDIINAQSYLPEGTGPEILRYWFPSDGKGMVDNDMLVTLKGGKNPVLAHLFINQML
                     EPEVAKENFSAIGYQPPQNSITPDSLVKEEFIPENLRSAIVKPEYFDTGYRLLELDPT
                     NEAAWRNVWQAFNAGGS"
     gene            19104..20045
                     /locus_tag="VFC01_23740"
     CDS             19104..20045
                     /locus_tag="VFC01_23740"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN82662.1"
                     /translation="MAVPVIAAPSRSDGPRRQRSSGIWPALAAPGIIWLAACFVFPLY
                     VVLCIVFGQIDPLFRTPVPVWNPLQWDPTQFTYVLTHIVGTHGVFGPPLLRTAVYVLT
                     ASALCLLIAFPVAYYVARLSGKRKGLLLALLIAPFWISYMMRMFAWVNLLQNDGIVNK
                     VLTFGGLFNVHVDWLTGQPVVVILGLVYGYVPYMILPLYAGLDRLSQPMLEASRDLGA
                     DRFSSFWRVTLPLCRPTIVAAVLLTCLPMLGDYFTNDMLSASPKTTMVGNLINDGVQA
                     PGQTGQAGAFVMLVFLVALLPMLYYIRVTSRGSEVST"
     gene            20042..>20870
                     /locus_tag="VFC01_23745"
     CDS             20042..>20870
                     /locus_tag="VFC01_23745"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN82663.1"
                     /translation="MRGPVTWWNDPWRPPRFLIGITAGYLAWSLLPVAIAVLYSFNDG
                     RSRTTWQGFSFRWYWGDATLSVWHNDALHTALLQTLKLGVVTTLITVPLGVLFAIGID
                     RWRGRLPAGANFLMLISFVLPEVLLAVALLFVITTIALPIHLGTTAQVIGLITFQVSY
                     PAVLVRARLATIGPQYEEAAMDLGASRLGALRRIILPMLMPAIFASTVLVFADVIDDF
                     VLVRYLSGDAATEPVSVKIYNTARAAPTPALNALATLLLLAALTAVVVGFLVYRYMTR
                     "
BASE COUNT         3540 a         6979 c         6847 g         3504 t
ORIGIN      
        1 tgatgaatgg ggtgaagccg gacaccatcg ccgagatctg gtaggccagc gaggctcccg
       61 acgaccgggt gttggccgtg aacaactcgg ggaaccacgc accctgcgcg ccggccagcg
      121 cgttctggca gacaccgtag gccaccacga tggtggcgat gatcagcagc tgcaggccgg
      181 tattcaccgt caggaacatc agcacgccga acgggatacc gaaggcgcag atccacatat
      241 agacggggcg acggccgatc ttgtcggtga gccgggccca ggccatggtg gcgacgatgc
      301 cgatcgccga cgcgatgcac agcgcggtca gcgtttcgga tttggtggcc agctcggcgt
      361 tcttcagata cgagatcatg taggtgatcg acaccgcgta gccggcggtc tcggcgatgc
      421 gaaggccgat gccgcgcagg atgttgcgcc agtcttcctt gatcaccttc acgatcgggg
      481 acttgacgat gtcgccgtgc tccttgacgt cctcgaacac cggtgactcc ggcaccttgg
      541 cgcggatgat caggccgacg atgaccagga tgatgctggc caggaacggc aaccgccagg
      601 cccagtcgcc gggcaggtgc acgctgacca aaaacaccag gttggccagc agcagaccga
      661 ccgggaagcc ggcctgcacc attccggtgt agcggccttt ttgtcgccat ggggcgtgct
      721 cgtagctcat caggatggca ccgccccatt cggcgccgaa agcgatgccc tggatgatgc
      781 gcaccgtcac caacaggatc ggggccagga tgccgatggc ggcgtaggtg ggcagcaggc
      841 cgatgacgaa ggtggccaga cccatcacga tcaacgaggc caccagcacc ggcttgcgtc
      901 cgagcttgtc gccgaggtga ccgccgatga tcccacccag cgggcgggcg gcgaacccga
      961 cacccagcgt ggcgaacgac gccagggtgc cggccagcgg gctggccccc gggaagaacg
     1021 cgtggccgaa gtacagggcg gcggcggtgc cgaacccgat gaagtcgtag gtctcgatga
     1081 ccgcgccgac gccggagccg atggcaacct tcttggcgtc cttggtgccg tgtaccggcc
     1141 cgcgcatgga tagagcggca tcgctcatga gctgatcctt tcaaaagccc tggtccggga
     1201 ctagtccgat gtcactgtca acggtcaaca gtgcgctagg tcacccagtg tgaccccgcg
     1261 cagacgcaat gtcaacagtc aacagaagct ctctaacatc agttgactgt taacagtcga
     1321 ctaggttaac gtggtcgtcg tcacaacgac gatgcaagga cctggaatga ttcacagccg
     1381 cctcggctgc tcgacgatca gctttcgaca cctatccctc gaacccgcac tcggctggat
     1441 cggagcgctc gggttcgccg aagtcgatct cggcgccttg cccggagtct gcgaccacgt
     1501 tccgtacgtg ctcgaccgcg atgccgtcga cgccgttgcc gacaccgtgc gcgcctccgg
     1561 catccgtgtt cgttcgatca acggtgacat cggcgacctc aaccgcccgc tggacaccga
     1621 cggccgcgcc tcccgcgacg gtcacctcga gatgctcctt gcgctgacgg cggcgaccgg
     1681 tgcgcacgcg ctggtgctgc cctgcggcgc gcttgatcac cagccgctcg gatcgctcga
     1741 cgacgacctc gacttggtgg cgcaggaatt gacgcgctct gccgaacagg ctgcgggcca
     1801 cggcgttgag atctggaccg agtcccttca cttccaccgg ctctgttgga atctcgaacg
     1861 cgcacaaaaa ctcaccgacc ggctcgcagg caccccggta caggtggtga tggacttcag
     1921 ccacatcgtc gcttccggcg gcgatcccgg cgagttcgtc gatcgcttcg ccgggcgcat
     1981 cgcgcacgtc cacatccgag acgccgtgcc gggcaacatc aacctgtccg tcggccgcgg
     2041 cgacgtcgac ttcggtcgcg ggctcaaggc gctcgcggac gcgggctata cgggtcattt
     2101 cgcactggag ctagagaccc gcgacatcac cgactcccaa cggcctgccg ccaccgccaa
     2161 aacggcccat ctgatttctg acctcatctg aaggagaaac accatgtccg aaaccaaacg
     2221 caccgccgtc gtcaccggcg ccacctcaga ccgcggcatc ggcatggccg tcgcgcagcg
     2281 gtatgcacgg gaaggctggg cggtcgtgat cctcgacctc gacggcgaga agtcggcgaa
     2341 agtggccgcg gagatcggca accaattcgc cgttcccgca ttcggttacg cgatcgacgt
     2401 cacatcggaa gagtcggtcg ccgccgcgga acacgccgtc gccgaggagg tacggtcggg
     2461 cgctctgccg ccgcttggcg cgctggccaa catcgcaggc atcacctccc cggtgccgtt
     2521 cctggagacc acgctggatc tatggaacac agtgatggcc gtcaacgcca ccggaaccta
     2581 cctggtcact aaagccttcc tgcccgccat gatcgacaac ggatggggtc gcatcgtcaa
     2641 catgtcctcg gtgtccgcgc agcgcggtgg cggcgtgttc ggcaaggtgc cttactcgtc
     2701 ggcgaaggcc gcgatcctgg gcttcaccaa ggccctggcc cgtgaaatcg ccgacaccgg
     2761 tgtgaccgtc aacgccgtca cccccggcgc cgtcgacacc aacatccgcg tcggttcgac
     2821 cgacgatcag gaggccaagc tggcgcgcga tattccggtc ggtcgcaccg cgaccaccga
     2881 ggagatcgcg gcggtcatca ccttcttgtc cagcgacgac gccagttatc tgacgggcac
     2941 caccgtcgat atcaacggcg gcagccacat tcactgacgg gaaagacgat gacacggttg
     3001 tacaacgacc ccgccacctt caccgaggac atgcttgacg gcttcctcga cgcgaacgcg
     3061 cgctacgtgg tcggtgtcga aggtggagtg gtgcgagcga cgacgacgcc gccgggcaag
     3121 gtcgcggtcg tcgttggcgg cggatccggc cactacccgg cgttctgcgg tgtggtgggc
     3181 cagggtttcg ccgacggcgc cgtcgtcggc aacatcttca cctcgccgtc cgcgcgtgaa
     3241 gccgcgtccg tcgcccgtgc cgcgcacggc gacgccgggg tgctgctgat caccggcaac
     3301 tacgcgggcg acgtgatgaa cttcgggctg gcaaccgagc aactccgttc cgaggccatc
     3361 gacgcccgct acctcgtcgt gaccgacgac atagccagcg ccgcgcccga cgacatcacg
     3421 aggcgccgcg gcatcgcggg tgacttcacc gtatttcgtt gtgccagtgc cgctgccgaa
     3481 gaaggcgccg cactcgacga tgtcgagcgc gtggcccgca agaccaacga cgccacccgc
     3541 acactcggtg tcgcgttcga cggttgcacg ctgcccggcg ccgaccgacc gctgttcacg
     3601 gtggcgcagg gcaccatggg cttgggtctc ggcatccacg gcgagcccgg ggtgtccagc
     3661 cacgacatgc ccaccgccgc cgacctggca gcgctgcttg tcgacggcgt cctcgctgaa
     3721 acacccgctg gcgcaacgga tcgcgtcgcg gtcatcctca atgggctggg ccgtatcaag
     3781 tacgaagagc tgttcgtggt ctggaagacg gtcgcggcac gactcgccgc ggccggactg
     3841 acggtggtgc tacccgaggt cggtgagctg gtcacgagcc tcgacatggc cggttgctcg
     3901 ttgacagtga cgtggctcga cgaagaactg gagcgactgt ggtccgcccc ggcggataca
     3961 ccggcctatc gtaagggtca aatcaccggc gctgcaggca ctttacggga acgcagccac
     4021 acgcccacac gcaactccgc tacgacaccc acgttcatgg atgccgacga gtcgtcacgc
     4081 gtcggcggcg agaacgtcgc tcaggcactc aggaagattg ccttgcggat ggttgaggcg
     4141 gaaagcgaac tcggccggat cgacgcggtg gctggggacg gcgatcacgg ccgcggcatg
     4201 gtccgcggaa ccgaggccgc cagcgctgcc gccgactcga cggccaatcg cggcggcgga
     4261 atcggttcgg tgctggcggc ggccggtgaa gcctgggcgg cgaaagccgg gggcacctca
     4321 ggagtgctgt ggggcgcggc gctgtctgcg gccgcccgca agctcggcga tcagggcacg
     4381 ccgaccgatg cggatgtggc cagcgcgttg cgcgcggggc acgacgccct tgttgaactc
     4441 ggcggtgcgc gccgcggcga caagacgatg ctcgacgcgc tcggaccatt cgtcgaatcg
     4501 gtcgagtccg ccgtggggtg cggaatcgat tggcgcaacg cgtggctcgc atcggtcgac
     4561 gtcgccggta agagtgcggc gggcaccgct gacctacggc cccgcgtcgg ccgggcacgc
     4621 ccgcttgccg agcgcagcct cggcaccccg gacgccggcg ccatctcgct ggtgatgtgc
     4681 attgacaccg tcgccggatt cttccaacac gaaggagcat cgcgatgagc ctgcggatcg
     4741 tcgtcggcag cgacgacgcc ggactccagt acaaacagat cctgcaccga gacctagaga
     4801 ccgattcacg ggtggcgtcc gtcgtggacg tcggcgtcgg acccgatgag cacactgcct
     4861 atccgcatgt cgcggtgacc gctgcgcgac tggtggccga cggcgaggcg gaccgcgcat
     4921 tgctggtgtg cggcaccgga cttggggtgg cgatcagcgc caacaaagtg ccggggatcc
     4981 gcgcggtcac cgcgcacgac agcttctcgg tcgaacgggc gatcctgtcc aacgacgccc
     5041 aggtcttgtg cttcggtcag cgggtcatcg gcatcgaact ggcgcgccgg ctggtcaggg
     5101 aatggttgag ctaccggttc gacccggaat cggcatcggc ggccaaggtc gcggcgatct
     5161 gcgagtacga gcccgcgtaa ccgggcgtca cggagcaaga tatgcaccgt gatcccgctg
     5221 acatggaagc acgtcgagac acagcccgag gacgacttcg tcgtcgcccc tgacgagcga
     5281 ctgagctggg cccgcaccat cggcctcggc gcccagcatg tggtggcgat gttcggcgcc
     5341 accttcctgg tcccggtgct gacgggcttt ccaccagcca ccacgctgct cttctccggg
     5401 atcggcactg tgctcttcct gctgatcacc ggcaaccggc tgccaagcta tctgggctcg
     5461 agcttctcgg tgatagcacc ggtgacggcc gccgtggcgt cgcacggcac cggcagcgcg
     5521 ctcgggggcc tggtcgcggt cggcgtgctg ctgatcatca tcggcgttgt cgtgcacttg
     5581 gtcgggactc actggatcga tgtgacgctg ccgccggtgg tgacgggcgc gatcgtcgcg
     5641 ctgatcggtt tcaacctggc gccggccgcg aagtcgaact tcgagaaggg cccgctcgtc
     5701 gggctcgtca cgctggtgtt gctggtcgcg acactcgcat tctttcgcgg catcatcggc
     5761 cggttggcga tctttctggc cgtcgtcgca ggctatgtgc tggcgctgat tctcggcgag
     5821 gtcgatacgt cggccatcaa ggcggcgccg tgggtcgggc ttccggagtt ccagaccccg
     5881 acgttcacgg tcgcggtgct gccgatgttc ctccccgcgg tgatcgcgct ggtggccgag
     5941 aacatcgggc acgtcaagtc ggtgggccag atgacggaca cagacgtcga cccggtgatg
     6001 ggccacgcgc ttgcggcgga cggggtggcg acgacattgg ccggcttcgg cggcggctcg
     6061 gcgaccacca cgtacgccga gaacatcggc gtgatggcgg cgacccgggt gtactcgacg
     6121 gccgcgtatt ggatcgccgc cgcggtggcg atcgcattgt cgttgtgccc caaggtgggt
     6181 gcggcgatct cggcgattcc gccgggtgtg ctgggcgggg cgacggtcgt gctgtacggg
     6241 ttggtgggcg tcctcggtgt gcgcatctgg ctgaccaatg atgtcgactt ctccaagccg
     6301 ctgaaccaga tgacggcggc catcccactg atcatcggta tcgccgattt cacctggcaa
     6361 gcaggcgatc tgacgttcac cggtattgcc ctgggctcga tcgcagcgct gctggtgtat
     6421 cacggcatgc ggctgctggg gttcaggaag gcgcgcgaac cgatcccgca acgcagcaca
     6481 taaggcctac agttccaacc gatgcgtcgc acctggacct ggacattcga tctgccgccg
     6541 gaccggctct ggccggtgct ggcggacacc gaccggttca acgaggcgat gggcctgccg
     6601 ccgtacacgc tggagcagac gccgcaacca aacgggacgg tgcttcgccg cgggcggggc
     6661 aagaccgcgg gcttcaacct cgaatgggag gaaaagccgt acgagtggat cgccggccgg
     6721 cacttccgcc agtcgcgggt attcacgaag ggcccgttcc gccgcttcgg ccccgtattc
     6781 gatctcgagc ccgacggcaa agggggctcg cacgtcacct acgcactcga gtgggagccg
     6841 ttgagtctgg tcggccgcgc cttcggcgcg cggctggcgg cccaggcggg cgaggcggtc
     6901 ggcaagcgcg tcgtgcaggc ggtcgccttt gcacaggggg agcaagcggg cgaacgcctg
     6961 acaccgtttg acttgccgcc gcctgacctg cctcccggcg cgcgcgagcg agctttcgct
     7021 ctggcacgcg aaatcgacaa cagcccctac ggaaatgggc tcggcacacc gctcgcccac
     7081 atcgtgctga acggcatggc caccgatctc acccatctca agcccaaggt gctggcgaaa
     7141 aaactcggcg ttccgccgcg gaaggcaatc gaggcctgcc ttgccggtgt gaaagcgggg
     7201 ctgctcacga tgaagtggga cctgctctgc cccaactgtc gcggtgcaaa gctacgcgtc
     7261 gccgttctct ccgaattgcc gcgtggcgcg cattgtccct cgtgcaacat cgattacgac
     7321 cgcgacttcg agaagaacgt cgagctctca tttgcgccgg cacccgctgt gcggccgctg
     7381 catgacggcg gcttctgtct gtccggcccg atggggacac cgcatgtcgc ggtgcaggtg
     7441 ttgctgagtc cgggcgaacg gcgaaacgta tccgtcgagc tgccaccagg gtcgtatcgt
     7501 ctgcgcacgt tgcatcccgg cgtgtttgtc gacattgact acgagtccgg gcctttccct
     7561 ggcttgcgcg tcacggcgtc gggggtggag tcgctgaatg ccacggatca gtcgaacgaa
     7621 gcgggaagcg tcacgttcgt taacgatgcg gagttcgagc tcgcggccct gatcgaggat
     7681 cgcacgtggg cgcgcgatgc gctgatggca ccggaagtga tctcgctgca ggcgtttcgc
     7741 gatctgttcg ccgaggcgac cctgcggccg ggcgacgatg cgggcgtcag ccaggtcgca
     7801 ctgctcttca ccgacctgcg gggctcgacg gcgctctatg aacgggtggg cgacgccact
     7861 gcctacaaca tggtgcgaaa acacttcgca ttgctggctg cgctcgtgcg cgaccacgac
     7921 ggcgcggtgg tcaagacgat tggcgacgcg gtgatggcgt cgttcagcga tccggcacag
     7981 gcggttcgcg cggcactggc catgcagggg gagatcggct cgactgacct ggttctgaaa
     8041 gtgggcgtcc atgccggccc cagtgtggtc gtgaccttga acgaccgcct cgactatttc
     8101 ggatcgactg tcaacatggc cgcccgcctg cagggccaga gcatcggcgg cgacatcgtg
     8161 ttgtcacacg ccgtggccga cgatcccgcg gtgcgggaag agatcgccgc cataccgcaa
     8221 cgccaagaga cggtcgcgct caagggtttt acggagccgg tcggtttcgt ccggctgctc
     8281 tgaacatgcg ccgactgcac ggttgttgta cacaaacgtc gagtaggccc gcaaacaatc
     8341 gtgcgctcgg cgtcaggccg gatcgagcag tgcgccgggg ccgcgggtca tcagctgacg
     8401 cccgacgatg atgcgctgga tctcgctggc cccctcccag atgcgctcca cccgcagttc
     8461 ccggaacatc cgctcggcga cgttctcgcg catgtagccc cggccgccga agatctgcac
     8521 cgcccggtcg gcgactcggc ctgccatctc ggagcagtag agcttcgcca tcgaggcctg
     8581 gccgtgcagt gtcttgcggt catggccggc gtcgatcgac cgggccactt cgtagagcat
     8641 cgttctggcc gcgaacagtt cggtcgcgca gtcggccagc atgcccgcca ccaactggtg
     8701 ttcacccagc ggccggccgt cgacgatccg gttctgcgcg aacgccgtgg cctcgtcgac
     8761 cagccgctgc gcggccccaa cgcacctggc agccaccatc attcgctcga accggaacca
     8821 gtcctgggtg aacgtcatcg gcacgccctc cgtcacgacg aggttggcca ccggcacgcg
     8881 gacgtgcttg aacgccacga tcgggtgctc gtcggcgatg tgatgcgaat actgcggcgt
     8941 gcgaaccact tccaccccgg gccagggcag atcgaccacg agtagcacat ggtcaccacc
     9001 ggtgagcacc gcttcgacga agacgtagtc ggcgagattg aacgacgtga cgtgccactt
     9061 gacgccgtcg atcacgtact cgtcaccatc gcggtgcgcg gtcgtctgca gtgcggacac
     9121 gtccgagccg gcgaactcct cggtgatcgc gtatgcctca tgcttctcgc cgcgcaccga
     9181 cggcagcagc caccgctcgc gctgatagtc gctggcgacg tcgacccacc attgcggcgg
     9241 ggtggccatc acccagccca gcccgttggt tacccgcccg cactgctcct gcaccaacac
     9301 ctgctgcaaa gccgtgcagc cggggccgcc gaccgaggtc ggcatgttcg tcgcgtacag
     9361 acccaattcg attgcgcggg cttgatgctc ggccgtcagc tccttgggca ggatgccacc
     9421 cgcgagctcg gcttccacct cgtgcggcat caacgtctcg acgaacgcgc gcgccgtgtc
     9481 ccggatgcgc cggtcctgct cggtcaggcc atacatccac gcctccaccc tcttgactga
     9541 gcgttcagtc tatgccagcg ttccgtagta tgaccagctc ccgcagcgcc gaagtcgtgg
     9601 tggtgggcgg tggaacggtc ggggcatgga cggccgtgct gctcgccgaa cgcggtgtgg
     9661 cgaatgtcgt gctcatcgag gccagaacgc tcggcgatgg cgccagcagt cgtgccgcag
     9721 gcatggtgcg cgcccagggc ggcaccgaaa ccgcgatccg gctgggtctt cgcagccagg
     9781 agttctacgc cgccagtggc gatcgcttcc cgttgggctg tggctttgtg gctcagggct
     9841 atctgatgcc gtgctttacc gacgccgagg tggcgcaggc acatgagcga atcgcgctgc
     9901 agcggtcgct cggcctcgat gtggaatggc tgtccagcgc ggacatcgac gatcgccaaa
     9961 cgggcctggc gccgggggtg acccttggcg cgtcgtatgc acctggcgac ggctacatcg
    10021 acgcgccgcg caacgtgctg gcgtacaccg cagcattgac cgcggttggt gccgacgtcc
    10081 gcgaacgctg caccttcacc gggctgcgca cggcggacgg ccgtgtcgtc ggcgttgaca
    10141 cgtccgacgg cccgatcgac accgagcacg tggtgctgac cggtggcccg cagctcgccg
    10201 aggtgggtca gacggcgggc ggcagaatcc cggcgggggg cacccggcat caggtggtgg
    10261 tcaccgcgcc actgccgctt gacgtgaacc aattgccgat ggtgtttgac atcacctccg
    10321 gaatctactg gcgcccaggc gaatccggcg gcctgctgtg gggcatgagc aaccccgatg
    10381 agccaccagg cgtggcgatc gacttcgaca cggtgtacta ccgcaaggcg cgtgaccgca
    10441 tcgaggagct gtttccccca gtgcgcggac tcgggctgcg ccgggcgtgg gcggcgacga
    10501 ttgactacac gccggatcat ctgccgatcc ttgggccgtt gctcaccgac gacgggccgg
    10561 tggagggcac cgtcgtggcc agccccgccg gccacgggat gatgtggggt ccagcggtcg
    10621 cacaggtggc cgccgacctt acagccaccg gtcgttgcga ctggcttgac ctcaccgatc
    10681 tcggcctgga ccgattcgat gccgaaggca acagcaggct cgcgcccgag ccgatctcgc
    10741 tacctttccc ggaaagcgtt gcgcgctaag cgcgagcagt cgcaaatgga cccgacacgc
    10801 cgacaaaatg ggtcactttg cgttccccag ctgacgcgtg ggcccagctc gcggaacaac
    10861 gagagcgtcg accgcgacca cgccaccggg tgacgcaaca acgggattcg cctcgaccgc
    10921 atcgaacagt tcgcccagat ccacggccat ctgcgagaac cgcaccacga cgtcggcgag
    10981 tccgtcgaca tcgacacccg actgaccgcg ccaaccgcgc agcagcggcg cgacgcgcaa
    11041 ccgctcgagt aggcgtcggg ccgcggcatc cgacaccggc ggacacgcga cgacgcggtc
    11101 gccgagcagt tcgaccaacg tgccgcccgc ggcgacgacc accagcgggc cgaaggcgtc
    11161 gtcgcgcacg aacccgaccg atacctcgac accgtccgcg accatcggtt gcacggtgac
    11221 cgctgggccg agcgaccccg ccatcgccgt atacgccacg ctcaataccg cttcatccgc
    11281 cagattcaag acgacgccac cgacgtcgct cttgtgcagg gcgccaagcg ttttcagcgc
    11341 gacggggtag cccagttcgt cggcagccgt cagcgcctcg ccgatcgagt gggcggtgcg
    11401 tgattgcacc acgggtatcc cataggaggc aagcagctca aacgcatcct cggggattcg
    11461 ccgtcctgac cgtgcgggct gacctggccg ctcgaccggc agcggccagc gcaccaggtg
    11521 gcccagcgcg gccagaccgc tgcgaaaccc ctcaagcacc ggaactccgt tgtcgcgcag
    11581 ccgttgcgcg gtgggtgcgt cgatcgccga cggtaccgat gccagtacgg ccaacggcgc
    11641 gtcggtcgat ctcgcgacgt ccagcactgc gtcggcgtag gcggtgtcac cgtcgaactc
    11701 ggtgaccaaa tccaccgcaa gcgccgtgac acccaccccg ggatcgtcag acatcgcttg
    11761 cacacaggcg ccgaacaggt tgcgggtgct ggcgccggta ccccagacat ccagtgggtt
    11821 ggtcggcgcc agaccgtcgt cgagcatgcc gccgatggtg gccaacgtct cgtcggccaa
    11881 gtcggcgaac ggcgccgtca gctcatgcgc gaggtcggcc agaagcgcac gctccgcccc
    11941 cgagtcgtgc accgtcgcca ccccaccacc attcgaccgc cgtcccgccg agaacaattc
    12001 aaccgtgtcg gtgaactccg ccagatcgct cacccgcatc gcaccggtgg cgtcgcacaa
    12061 ggcttgccac gccgcgtgtt caccggccag cgccccggaa tgcgcggcga ccatcgcacg
    12121 gccgcgcggc gacccgccca ccgcgagaac caccaccggc acgtcttgct cggcggcgcg
    12181 tagcagggat tgccgcagcc gtggcacaga gcgcggcgtc tccatcaaca gcgagatgat
    12241 ccgggtatcg ggatcatcga gcgcatagtc gacgtagtcg gcggtgtccg tcacgagctc
    12301 ctgtccggac gacacggcaa gccggaaccc gaatccacga cgcgctcgca acaatgtcga
    12361 gaaggcggac ccagagtggg tgacgagagc aatcccgccg gacggcaacg gatctggctc
    12421 caggtacccg agtgcccgca cgccaagcgc gttgttgacg aatcccatgc agccggcccc
    12481 gcacagcgcc atgcccgcct cggccgcggt cgtcgcgatc ttctcgcgca gaccgtgcgc
    12541 ggtcccgaac agcacggccg accgagcacc aacggctgcg gcgagttcca gttgctccac
    12601 cagcgccgag tcggggaccc cgagtagcac cacatcgacg ggttcgtcga gatcggcgag
    12661 tgagggcgcg caggctagct cgccgatgcg gtcgtagcgt gggtttacca agtgcacacg
    12721 ggccgaaccc cgttgcgcct cgaggaccat ccgcgcgccg aagctgtcgg ggcgggcact
    12781 ggcaccgacc acggccatcg accgggcgcc gagcatggca gcgaccgttg cgcgacgatg
    12841 ctcggtcatc cgcggaagag acggcgcagg ccccgtttct gatcggccag cgccgcgcgc
    12901 gccgcctgcc agccggggat accgcacacg ccgccgccgg cgtgtgtgcc cgagcttccg
    12961 ttgtacaacc cggcaatcgg ggtgcggtaa tcggcgtagc ccggggcggg gcgcatgtgg
    13021 aacagctgct ccaacgacag ctcgccgtgg aagatgttgc cgccgatcag cccgtagtcc
    13081 cgctccatct cgtacggacc gacgatgtcg aggtgcgtga tggaggactt gaagcctggt
    13141 gcgacttggt cgtaaaggtc gatgagccgg tcggcgtagg cgtcgagctc ttcggtgtgc
    13201 ggcgcctggc tccagtccgc aggcacccac tgcgtgaaca acgacatgat gtgtgtgcca
    13261 tcgggattca gcgtcttgtc cagcgtggtc gggatgacgc cgtcgctgaa cggcagtagc
    13321 gcgggccgac ccgcgcgcgc gtcctggaac gccgcctcga tgtactccat cgtcggcgcc
    13381 atctccaccg atcctgtgtg gtgctcggcc atgccgcggc tcgggtcggc ggtgaagttg
    13441 ggcagctcgg cgagtgccag gttgatcttg acgacaccgc tgcgggtctt ccaatgctcg
    13501 atgtcggtga cgaattcgtt cggcagctcg gtgcgtggaa tgtgttcgag gaaagctgtt
    13561 ttcgggtgaa gcgtggtaat caccaagggc gcgtggatct catcgccgtt ttcgaggacg
    13621 gcgccgtgta ctcggccgcc acggacaagg agccgcgcga cgcgggcgtt ggtacggatc
    13681 tcggcgccga agccgcgagc agaccgtgcg atcgcctcgg agacggcccc catgccaccc
    13741 tcggggtagc cccagctgcc gagttggcca tcgccgacgt cgccgatcga atggtgcgcc
    13801 atcacgtagg cggtgccggg ttcatacggc ccggcccacg ttccgatcac gccgttaacc
    13861 gccagcgcgc ccttgatttg cggtgattcg aaccagtcgt ccagcaggtc ggcgatgctc
    13921 atggtgagca gccgggtgac gtcggcggtg gtgcgcacgg tgatcccgcg ctgacgccac
    13981 gccagccgcg ccaagtccag caggtccgaa ggccgacgcg agccgatgtt cggcggcacc
    14041 tgggtcaaca acggacccat cacctcggcg atcccctcga gccatgcgtt ccacttgggc
    14101 caggcctcgg cgtccttctt cgaccacttg gccagctgct cataggtgcg ggccgggtcg
    14161 tcctcgtaga tggtcagcga gccaccctcg gggaacgcct ggtagtacgg gcccatcgga
    14221 tggaccttgt atccgtgccg ctcgagttcc agttcccgga cgatcgtcgg cggcatcagg
    14281 ctcatcacat aggacagccg cgtgacacgc aggtgcggtg cgtcctccca cggcgattcg
    14341 gtggtggccg caccccccag cgacccgcga ccctcgagca ccagcgttct ggcgcccgac
    14401 cgggcgaggt atgccgcgga gaccaagccg ttgtggccgc cgccgatgac gagggcgtcg
    14461 tacgtgttcg acatgaaagc cccactttcg tcctgactga ctgttcaatc taatcaagtg
    14521 ttacgctaca gacaagagca gcgctgcact gggagggaac acacaacgcc atggccgatc
    14581 ccgcacccgc aaccgagcag aacgaagccc ggcgcatcga aatgctccgt gccgcagcgg
    14641 agctgatctg tgagcggggt ttcggggata cccgcatcgc cgacgtcgcc aagcgcgcag
    14701 gcgtgagctc ggccctcgtc atctattact tcggcacccg ggaccgccta ctcgtcgatg
    14761 cgctgcggta ttcggaggag tcgttctacg aagcagccga gcagatgctg gccgaggttc
    14821 cgtccttgcg cgaacggctc tcgctgctga tccagtggac ctgcgtgccc gaaggcgcag
    14881 gcgagattcc cggtgcctgg ggcttgtggt tcgacctgtg ggcgcaggcg tttcggcacg
    14941 acgagatcaa ggccggccgc gccgaactcg acgcccggtg gcgcaagatg atcatcgatg
    15001 cgctcgattc gtcggacctc agctcgaaag acaagcgcat gttcgcgctg gagttcgccg
    15061 cgctgctcga tgggctgtcg atccaggtcg cgctggaaga cccgttggtg gactccgacc
    15121 tggcctatga catcgcgatg cacttcgcgg agcgtgaact caatctgccg cctgccaaga
    15181 agcggctgaa cggcaaggcg aaggccgcta agagggactg agcgcgccga cctgctcggc
    15241 caccggatcg gcgatcggca cggccgtgct gcggtgcagc acacgcaccg cgtcgtgcgt
    15301 gcacacgcag ttgaccttcg cgcccggctc atgggtcacc gaggtcgggc ctgcgtgatt
    15361 ggccacctcg acgaccaacg ctgtgggcgc gccgacgtcg acgtgtacct gcgtgcagtt
    15421 gccgaggtac accacctgtg ccacgacgcc ctcaatgacg ttgcggcccg gaccgattgg
    15481 ctcgccggga ttctgcagcg agatgcgctc gggccggatg acgatcgcag cagctcccga
    15541 tacggccgac gcgtcgaccg ccgcaccgag ccgcgtgtcc agcgccgagc acactgccga
    15601 tccgtcgccc gattccagga cgtcggcgtc gaaaatgttg gcagcaccca tgaatccggc
    15661 gacgaatgtg gttgccggcg ccgagtagat ctcctgtggc gggccgacct gttccacgcg
    15721 gccttcggcg agcacggcga tctggtcgct catggtgagc gcttcctctt ggtcgtgcgt
    15781 cacatagacg aacgtgatcc cgacctcgcg ctgcactgcg cgcagctcca gctgcagctg
    15841 tttgcggagc ttcgcgtcca gtgcacccag cggctcgtcg agcaggagca cgcgcggccg
    15901 cagtaccaag gcgcgggcca acgcgaccct ctgctgttga ccgccggaaa gctgcgcggg
    15961 ccggcgtttg gaaaagtcgc tcatgcgaac cagttccagc gcttcgccga cacggcgctt
    16021 ggcgtcgtca cgcgaaatct tgtggtactt caatccgaat gcgacgttgt cccacaccgt
    16081 catgaacgga aacagcgcat aggtctggaa gacggtgttc accggacgct tgtgcggagg
    16141 atcgctcacg acgtcgcggc cgtcgagttc gatacggccg gagtccggct tttcaaagcc
    16201 tgcgatcatc cgcaatgtcg tcgtcttgcc acagcccgac ggtcctagca gcgaatagaa
    16261 ctggccggcc gggatgtcga gatcgatgcc cgtcacggcg gggactccgt cgaacgattt
    16321 cgccagctcc tgcagccgga tggcgccgcc agtcaacggc gcgagtcccg ggtcagggcg
    16381 ccaccgctcc tgggccgaac cgcgacaagg tcgcggagaa ccgggcgaag ttggtggcga
    16441 agcggtcgac atcgtcctcg tcgtgctgaa ctgacaacag ccattgctcc accttccccc
    16501 atggcgggag aaatacaccg ccattgtgct gcatcagcca gtgcaagtgg ccgagtcggg
    16561 cgtcgatctg aagaaaatca cggaattccc gcactgggtc gcgccggaaa gtgacacacc
    16621 ccttggcgcc gacggtcacc acctgccagc cgaaaccgtg ttgggcgatc acggcttcca
    16681 atgaggtgcg gagccggttg gcgagcgcct cgaggtgcgt gtatgcctgg ggggtgagca
    16741 catcggtcag ggtcgcgcgg gtggccgcca ttgagagcgg attgccgttg aacgtaccca
    16801 cctgctcgta acggccgtcg gcgatcgccg acatgacggt ggacgtaccg ccgatcgctg
    16861 caaccgcgat tccgccgccg agagccttgg ccaggcacac catgtcgggg acgacccctg
    16921 tcaaagcggt gacgccgccc ggccccgtgg tgaagccggt cttcacctcg tcgaagatca
    16981 acagcgcgcc ctcggcgtgc accaagtcgc ggatggcagc gaggtatcca tcgtctgggg
    17041 ggatgatgcc tgcgttcatc atcaccggtt cgaggatcat ggccgcgact tggccgcgat
    17101 gttcggcaag cgcccgcgcc accgcatcgg gatcgttgaa gggcacgacg atcaccagat
    17161 cccttatcgc ggaggcgatt ccagagttgc caggcactcc gatgggatgg tcgcgcggac
    17221 ctatctcgtc agcgtcgggc agtaccgaca cctgcaccga atcgtggtgg ccgtgatagc
    17281 agccctcgac cttgatgatc agatcgcgtc cggtcaacgc gcgggcaagg tgcaccgcgt
    17341 ccatggtcgc ttcggtgccg gagttggcga atcgccacag cggaaggccg aaacgacggg
    17401 acaactcgcc tgcgatccag atcgcatctt cggtcggctg ggcgaaatgc gtgccgcgac
    17461 gcacccggtc gctgaccgcg gcgacgatcg ccggatgcgc atgcccggca atggaagcgc
    17521 cgtaaccgcc gtgcatgtcg acgtactcgt tgccgtccac gtcgtagacc ttggagccgt
    17581 tgccgtggct catccagacg gcttgtggtt cggcgatctg ccaattggac gtcgcaccgc
    17641 cggcgagatg ttcgcgcgcc tgcgcgatca attcggtgct gcgcggctga cggcgcagaa
    17701 acacctgctc ctgtcccgcg atcagctggt caacgatgtc ggtttcctgg ctttgcacca
    17761 ccgacgaaga cacggtcaaa cctccgctcc gaacggttga ctgagcattc agtctatgcg
    17821 agagttccta ccagcgacaa ggcgtgaaag caacggggag gcctcagatg ccacagccca
    17881 ccagtcgacg acaattcctg gcgcgcgccg caattctcgc cgcaagcgcc ccgacgctcg
    17941 gcgcgttctt gcaggcgtgc tcgaagaatg cgccgtcatc gtcgcagccg accatgacga
    18001 tcgcggcacc cgacaacccg gtcacgtggc ccatcgccga cgacaacaaa cccatcgccg
    18061 acggcctcgc cccggagaag ggcgcgacgc tcaagatcta caactacgcc gactacctca
    18121 gcccccaggc catcaaggcc ttcgaggacc agtacgcgat caagatcgag gtctcgacgt
    18181 tcaatgacgg cgacgaggcc atcaccaaac tccgcagcgg ggtcgacttc gacatctaca
    18241 acgccaacta caccgagatc agccggctgg tgaacggcgg actactacgg ccgctgaacc
    18301 actcctacat ccccaacatc aagaatgtct ggccgggctt caccaacccc tggtacgacc
    18361 aggagtggcg ctacaccgtg ccgtacacga tctacaccac cggtatcggc tggcggagcg
    18421 atcaggtgcc cgccgacatc ggcgcgctga agaatcccta cgactcgctg tgggatccgc
    18481 agtacaaagg taagacggcc gttctggacg acttccactc cgcgatggcc atggtgcttc
    18541 tgcgccaggg gattacagac gtcaacacct cttcggaggc tgacctgaag atggtcggcg
    18601 aacaactcaa cgcgttggtg aaggccacct cgccgaaggt gaccatcacg atgtacaacg
    18661 atcttcccgc tgggcagctg ggtctcgcgc agatgtggtc gggcgacatc atcaacgcgc
    18721 agtcctacct gcccgagggc accggcccgg agatccttcg gtattggttc ccctcggacg
    18781 gcaagggcat ggtcgacaac gacatgctgg ttaccttgaa gggcgggaag aaccccgtcc
    18841 tcgcgcatct gttcatcaac cagatgctcg agcccgaggt ggcgaaggag aacttctctg
    18901 cgatcggcta ccagccgccg cagaactcaa tcaccccgga ttcgcttgtg aaagaggagt
    18961 tcattccgga gaacctgcgc tctgccattg tgaagcctga gtacttcgac accggctatc
    19021 ggctactgga gctcgacccg accaacgagg ccgcatggcg caacgtgtgg caggccttca
    19081 acgccggcgg atcgtaagaa ccggtggcgg ttccggtaat cgcggcgccg tcgcggtcgg
    19141 acgggccgcg tcggcaacgt agcagcggga tctggccggc acttgccgct cccggaatta
    19201 tctggctggc agcatgtttc gtcttccccc tgtacgtcgt tctgtgcatc gtgttcggcc
    19261 agatcgaccc gctgttccgc acgcccgtcc cggtgtggaa tccgctgcag tgggatccca
    19321 cgcagttcac ctacgtgttg acccatatcg tcggcaccca cggagtgttc gggccaccgc
    19381 tgctgcgcac ggccgtctac gtgctgaccg ccagcgcgct gtgcctgctg atcgcgttcc
    19441 ccgtcgccta ctacgtcgcg cggctgtcgg gcaagcgcaa gggcctgctg ctggcgcttt
    19501 tgatcgcacc cttctggatc agctacatga tgcgcatgtt cgcgtgggtg aacctgctgc
    19561 agaacgacgg catcgtcaac aaggtgctca cgttcggcgg gctgttcaac gtgcacgtcg
    19621 attggctgac gggacaacct gtagtggtga tcctcggcct ggtgtacggg tatgtgccgt
    19681 acatgatcct gccgctgtac gccgggctcg accggctgtc gcagccgatg ctcgaggcgt
    19741 cgcgtgacct tggcgccgac cggttttcgt cgttctggcg cgtgacgctg ccgttgtgcc
    19801 gcccgacgat cgtggcggcg gtcctgctga cctgcctgcc gatgctcggc gactacttca
    19861 ccaacgacat gctgtccgcg tcaccgaaga cgacgatggt cggcaacctg atcaacgacg
    19921 gtgtgcaggc cccgggtcag actggtcagg ccggggcgtt cgtcatgctg gtcttcctgg
    19981 tggcgctgct cccgatgctg tactacatcc gcgtgacatc gcgaggcagc gaggtgtcca
    20041 cgtgagaggg ccggtcacgt ggtggaacga tccgtggcgg ccgccgcgct ttctgatcgg
    20101 catcaccgcc ggttacctgg cgtggtcgct gcttccagtg gcgatcgcag tgctctattc
    20161 attcaacgac ggccgctccc gtacgacgtg gcaaggcttt tcgttccggt ggtactgggg
    20221 tgatgcgacg ctgtcggtct ggcacaacga cgcgctgcac accgcgctgc tccagacgct
    20281 caagctgggt gtggtcacga cgctgatcac tgttccgctc ggggtgctgt tcgcgatcgg
    20341 gatcgaccgg tggcgcggcc ggctgcccgc aggggcgaac ttcctgatgc tgatctcgtt
    20401 cgtgctgccc gaggtgctgc tcgccgtggc attgctgttc gtgatcacca cgatcgcgct
    20461 gccgatccac ctcgggacga ccgcgcaggt gatcgggctg atcacctttc aggtgtccta
    20521 tccggcggtg ctggtgcggg cccgacttgc gaccatcggc ccccaatatg aggaagccgc
    20581 aatggatctg ggcgcctccc gactgggcgc gctgcgtcgg atcatcctgc cgatgctgat
    20641 gcccgcgatc ttcgcgagca cggtactcgt cttcgccgac gtgatcgacg atttcgtgct
    20701 ggtgcgttac ctgtccggtg acgccgccac cgagcccgtc tcggtgaaga tctacaacac
    20761 cgcacgcgcg gcacctacgc ctgcgctcaa cgcgttggcc acactgctgc tgctggcggc
    20821 gctgaccgcg gtggtggtcg gcttcctggt gtaccgctac atgacccgcg
//
LOCUS       DASRJA010000537         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1987909,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000537 DASRJA010000000
VERSION     DASRJA010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1987909"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(410..691)
                     /locus_tag="VFC01_23750"
     CDS             complement(410..691)
                     /locus_tag="VFC01_23750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82664.1"
                     /translation="MIIDGFKVPQPTRFFTGASGSLSPLFNVQLPPGNLFGADETIVP
                     ELVLSPSGEQGYYLFVPLPPGEHVVHWVASGCLPGLTQDIRYQLTVVGR"
     gene            complement(751..>2077)
                     /locus_tag="VFC01_23755"
     CDS             complement(751..>2077)
                     /locus_tag="VFC01_23755"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZN82665.1"
                     /translation="AGGGGAALGGAIFNHGGGVTILNSTFTGNFVDRGRGGSGGLDGT
                     PGAPGTDAGGAIFSVAGALTVIHSTLSGNESTGEGAGIAVYKPTTGEATSFNLRNTII
                     ADSLPPTRECFVRNGPLVFGSGNLIDSASGFPFDARTPCPGIEQTGNPVLGALRINAP
                     GLTPTMEIPADSSAIDTGDPNASLDSDQRGVTRAFGAGPDIGAYERGNNPPVAVCTDV
                     TVTAGESCTANASVDNGSFDPDAGDSITLAQSPAGPYPLGANSVTLTATDSFGATNSC
                     SATVTVVDDTPPVVSSSVAKSLLAPMKNHDLVNVGLAASATDACGAPTSFSVEVAGDE
                     DDEATAGGVVFSPDARNIAVNTLRLRAERADSGDGRVYLIAVSAADGSGNTGFNCNTV
                     VVPRNTSAASKAAVNSQAAAALSYCQANNGALPPGYVIVGDGPVIGNKQ"
BASE COUNT          384 a          657 c          679 g          357 t
ORIGIN      
        1 gggagaacgc tcggcccgag ccgcgcggtc cgcagtcgcg tacttcgcgg agtcgggcag
       61 cgaggaagag aacaacctcc ccggaaacac cagacgtgaa gaaaccagtg aagttgagaa
      121 tcgtcggtcg cgacggctac aagctgcgcg atttctaccg ggacttccct ggcgcaggga
      181 ccagccttcc ggggcggatc gctgctgacc ttgcgaagcg agcagggcaa gcgccgtcgc
      241 cggagcaacc ctctggcgaa ccaggtcccg aatcgcagca ggaccctgcc aagccttgag
      301 cgtcgtcgcc agctacgccg cccactcggc ggacagcgcc tccgcctgct gaagcggctc
      361 tgaatgagaa gaggcggcgt atttaacgga acggtgcacc cgacgttggt cagcgcccaa
      421 caacggtgag ctggtacctg atgtcctgcg tcaatcccgg tagacatccc gatgcgaccc
      481 agtgaacgac atgctcaccc ggcggcagcg gcacgaacaa gtaatacccc tgctcgccac
      541 tcggactcag gaccagctcg ggcacgattg tttcgtcggc tccgaacaaa ttgcccggcg
      601 gcaactgcac gttgaaaagc ggcgacaagc tgcccgaagc gccggtgaag aaccgagtcg
      661 gctgcgggac tttgaagccg tcgatgatca tgacccgctc cccagatgtg aaagcctcac
      721 gagatcgttt ctcgctagaa tgaacatggc ctactgtttg ttgccaataa ccggtccatc
      781 gccgacgatg acgtaaccgg gcggcagagc accattgttg gcctgacagt acgacagcgc
      841 tgcggctgcc tgactgttca ctgctgcttt gctggccgca gacgtgttgc gcggcacgac
      901 gacggtgttg cagttgaacc cagtattgcc tgagccatca gcggcgctca cggcaatcag
      961 gtacacgcga ccgtcgccgg aatcagcgcg ctcggcgcgc aggcgcagag tgttcactgc
     1021 gatgttcctc gcatcaggag agaagacaac accgccagct gtggcctcgt cgtcttcgtc
     1081 gccggcgacc tcgaccgaga aactcgtggg tgcaccgcag gcatccgtag ctgacgcggc
     1141 cagccccacg tttacaaggt cgtgattttt catcggtgcg agaagcgact tcgcgactga
     1201 gctggagacg accggcggcg tgtcgtccac gacggtgacc gtggcgctgc aactgttcgt
     1261 tgcgccgaag ctgtcggtgg cggtcaaagt cacggagttc gccccgagag ggtaggggcc
     1321 agcgggagac tgcgccaatg tgatggaatc gccggcgtcg gggtcgaagg aaccgttgtc
     1381 cacgctcgcg ttcgccgtgc agctctcacc agccgtgacg gtgacgtccg tgcacacggc
     1441 gaccggtggg ttgttgccgc gctcgtaggc gccgatatcc gggccggcgc cgaacgctcg
     1501 tgtcacgccg cgctggtcgc tgtccaacga tgcattgggg tcgccggtgt cgatggcgga
     1561 actgtctgcg ggaatctcca tcgtgggcgt gaggcccggt gcgttgatac gcaaggcccc
     1621 aagcactgga ttccctgttt gttcgatgcc ggggcaaggc gtcctggcgt cgaacggaaa
     1681 gccagatgcg ctgtcgatga ggttgcccga cccaaagacc aacggcccgt ttctgacgaa
     1741 gcactctcgc gtcggcggga gactgtccgc gatgatggta ttccgcagat tgaaggacgt
     1801 cgcctcgccc gtggtcggtt tgtagacggc gatgccggcg ccctcgccgg tactttcatt
     1861 gccgctcagc gtcgagtgaa tcacggtcag cgcgccggca acggagaaga tggcaccgcc
     1921 ggcgtcggtg ccgggagcgc ctggtgtgcc gtccagcccg ccgctgcctc ctcggccgcg
     1981 atccacgaag ttgccggtga aggtgctgtt caggatcgtc acaccgccgc cgtggttgaa
     2041 gatggccccg cccagggcgg caccgccacc gcccgca
//
LOCUS       DASRJA010000538         5740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_305853,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000538 DASRJA010000000
VERSION     DASRJA010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305853"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VFC01_23760"
     CDS             <1..1185
                     /locus_tag="VFC01_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZN82666.1"
                     /translation="PEFDRLDRTEGGNPTLTIAGRGYHVDQASLLVTTPPGGLNATTL
                     RPQKPAGCTAADYGTVSVRNAITVVDATGCSVGDKQDVAISKGAVGLLVVSPPGPTAP
                     AGLFTPGYYQQLTIPVAVIDTDADNALRRTTAPVRLVLDSKPVMVKTRNVVAQTKTGD
                     AKNIVLAGAQLDSAARSPGINDNGSGVAALLETASALGAQPKTTNAVRFLFWGSETDG
                     PTKYVRGLGPDQLDDIAMYLSADMLGSPNAGYFTYDGDQSGQPNPDVPPQSVPAGSAG
                     VERTLAGYLNLAGVRPADMPLSRTTGYGPFLAAGVPIGGLTTGSSQRKTEVQARLWGG
                     RAGVPFDPNYQTPRDTIDNVDRKALSVMGQALAFAVGTYAQSVDGVNGVPLRDQRNRR
                     TP"
     gene            complement(1139..2311)
                     /locus_tag="VFC01_23765"
     CDS             complement(1139..2311)
                     /locus_tag="VFC01_23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZN82667.1"
                     /translation="MFKQGGPAPVPADALAAALAPFGQSRMLPREAYVDPAVFEWEQH
                     NIFTGWTCVGHASDLAAVGAQKAVGTGPNAMLLVRGEDDVVRAFANTCRHRGHELLAC
                     NAATKGRSIVCPYHSWSYRLDGRLRNAPGFRDVEGFEPDEFGLAELRLVNWLGWLFVD
                     SSGEDVDFATHVSGLDDVVGPYRPEDLTIVARHSYELATNWKVIAENYQECYHCSTIH
                     PELSRISPPTSGENLDLPGSWMGGWMSIVEGADTMSLTGKSGGVAIEGLSEHELRSVM
                     YLVGYPNLLVSLHPDYVMTHLMTPLAVDRTHVECAWAFPKDVAAKPDFDPSYAVDFWD
                     LTNRQDWAACESVQRGLTSPHARPGPLAPDEDGVYHFVTRVARAYAGSADPSAEHR"
     gene            complement(2417..4582)
                     /locus_tag="VFC01_23770"
     CDS             complement(2417..4582)
                     /locus_tag="VFC01_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZN82668.1"
                     /translation="MTRPLPAALVSGQPVTRVDGRLKVTGKAMYAADNPAPDLVYAAL
                     VCSTIARGAVERIDPAAAVRDRDVLRVLTDFGGVKLPYDIRQVSCLGQPVAVVVATTL
                     EAAMHGASLVEVKYRPGPQLTNIDAPQAEQKPGSRTRDYARGHADDELRAAAVVTDLR
                     YSIMRNNHNPMEVPATVASWDGDRLTVWDKVQSISGAQKSYADAFGVPGDQVRVISPF
                     VGGAFGSAGTVWPHQLLAAFAARQIRRPVKLMLTRKQYYSGIGFRPTSRQRMAIGADR
                     SGRVGAIVHEAHTETSRYNAYEDNLMTGARFLYGAPNMRSTYRVVPLDINPPTYMRGP
                     GATTGAFALESAMDDLAFRLGMDPVELRLRNEPDRDLTDNRPFSTRRLTDCFRQGADR
                     FGWARRNPTPRATRDGNQLIGIGTAAAVYHTSRSECAALARINADGTADIQCGTSDMG
                     PGTYTSMTQVAADALGLPFNRVRFALGDSRFPTAPSHSGSRTMASVGSAVFTTANMLR
                     DRFVRTAVLDPGSPLNGLRPEDVSIADGRMFRTSEPASGESYQDLLRRRGWGSLDSQQ
                     TWSPDDADERYSMSAYGAVFAEVAVDEALGTVRVRRVYGCYDAGRIINPKLAHSQAIG
                     GMVGGIGMALLEGTQLDYRDGRIVNANMSDYLVPVNADVPALDATYLPAEDTIADPLG
                     VKGLGELVIVGVPAAIANAVFNATGRRVTDLPITLEKLL"
     gene            complement(4579..5556)
                     /locus_tag="VFC01_23775"
     CDS             complement(4579..5556)
                     /locus_tag="VFC01_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZN82669.1"
                     /translation="MFPFQFTKAFDEAAALTAAASGGRYIAGGTTLIDLMRETVERPA
                     SVIDINALPYRNIDLQASRLRVGSLVRMSELAAHRGVRSQFPVIAQALELSASAQIRN
                     MASIGGNLMQRPRCLYFRDVSAACNRRTPGSGCAAIGGRNRTHAILGTSDACVATHPS
                     DLAVALIALDALVITRDGDGEHPIRIADFFRQPGGTPDREHNLAAGALITGIEVPVTP
                     EARRSGYLKVRDRESFEFALASAAVALDIAAGRVRAARVAVGGVGTVPWRLPAVEQAL
                     VGRPVSAALWRDAASNAAEGAKPLSENGFKVELIKRTVERQLATIAGLP"
     gene            complement(5563..>5740)
                     /locus_tag="VFC01_23780"
     CDS             complement(5563..>5740)
                     /locus_tag="VFC01_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZN82670.1"
                     /translation="ALQCGYCTAGQIMSGVGCIDEGHTRSPAEIREWMSGNICRCGAY
                     TNIVTAVANAARST"
BASE COUNT          959 a         1981 c         1863 g          937 t
ORIGIN      
        1 ccggagttcg accggctgga ccgcacggaa ggcggcaacc cgacgctgac aatcgccggt
       61 cgcggctacc acgtcgatca ggcttcactg ctcgtcacca ccccgccggg cggactgaac
      121 gcgaccacgc tgcggccaca gaagccggcc ggttgcacag cggccgacta cggcaccgtg
      181 tcggtgcgga acgcgatcac cgtcgtcgac gccacgggct gctcggtcgg cgacaaacag
      241 gacgtcgcga tatcgaaggg cgcggtcggg ctgcttgtcg tcagcccgcc agggccgacc
      301 gcgccggccg gcctgttcac gcccggctat tatcagcagc tcaccattcc ggtcgcggtg
      361 atcgacaccg acgccgacaa cgcgctgcgc cgcaccactg cgcctgtgcg cctggtgttg
      421 gacagcaagc cggtgatggt gaagacgcga aacgtggtgg cacagaccaa gaccggcgac
      481 gccaagaaca tcgtgctggc cggcgcacag ctcgacagcg ccgcacgcag tcccggtatc
      541 aacgacaacg gatcgggtgt tgcagcattg ctcgaaacgg cgtcggcctt gggagcgcag
      601 cccaagacca ccaacgccgt ccggttcctc ttctggggat cggagacgga tgggccgacg
      661 aaatacgttc gcggacttgg ccccgaccaa ctggacgaca tcgcgatgta tctcagcgcc
      721 gatatgctcg gctcgccgaa cgccgggtac ttcacctatg acggcgacca gtccggccag
      781 cccaaccccg acgtcccgcc acaatcggtg cccgccggat cggcaggcgt cgagcggacg
      841 ctggcgggct acctcaacct ggccggtgtg cggcccgccg acatgccgct gagccgcacc
      901 actggctacg ggccgttcct ggccgccggc gttccgatcg gcggcctcac cacaggatcg
      961 tcgcaacgga aaaccgaagt gcaggcgcgg ctttggggcg gaagggctgg tgtgccgttt
     1021 gatccgaact accagacgcc ccgcgacacc atcgacaacg tcgaccgcaa ggcgctgtcg
     1081 gtgatggggc aggcgcttgc gttcgcggtc ggcacctacg cgcagtcggt cgacggtgtc
     1141 aacggtgttc cgctgaggga tcagcggaac cggcgtacgc cctagccacc ctggtgacga
     1201 agtggtagac accgtcctcg tcgggagcca gcgggcccgg ccgcgcgtgg ggtgacgtca
     1261 gcccacgctg caccgactcg cacgccgccc agtcctgccg attggtcaga tcccagaagt
     1321 ccaccgcgta cgacgggtcg aaatcgggtt tcgctgcaac atctttcggg aatgcccacg
     1381 cgcattcgac gtgagtgcgg tcgactgcta gcggcgtcat caggtgggtc atcacatagt
     1441 ccgggtgcag gctcaccagc aggttggggt aaccgaccag atacatcacg ctgcgcaact
     1501 cgtgctcgga gagcccctcg atcgcgacgc cgccactctt gcccgtcagt gacatggtgt
     1561 cggcaccctc gacaatcgac atccagccgc ccatccacga tccgggcagg tcgaggttct
     1621 cgccgctcgt cggcgggctg atgcgggata gctcgggatg gatggtcgag cagtggtagc
     1681 actcctggta gttctcggcg atgaccttcc agttcgtggc gagctcgtac gaatgccggg
     1741 cgacgatcgt cagatcttcg gggcggtacg gcccgacgac atcgtctaaa cccgaaacat
     1801 gggtggcgaa atcgacgtct tctccgctgg agtcgacgaa caaccacccg agccagttga
     1861 ccagccgaag ttcggccagg ccgaactcgt cgggctcgaa accctcgacg tcgcggaacc
     1921 ccggcgcgtt gcgcagccgg ccatccagcc gatatgacca cgagtgataa gggcagacga
     1981 tgctgcggcc cttcgtggcc gcgttgcacg cgagcagttc atgcccgcga tgcctgcacg
     2041 tgttcgcgaa cgcgcgtacc acgtcatcct cgccacggac caacagcatt gcgttcggac
     2101 cggttccgac cgccttctgc gcgcccaccg ccgcgaggtc cgacgcgtgg ccgacgcacg
     2161 tccatccggt aaagatgttg tgctgctccc actcgaacac cgcggggtcg acataggcct
     2221 cgcggggcag catgcgggac tggccgaacg gcgccagcgc cgcggccagt gcgtcggcgg
     2281 ggaccggggc cgggccaccc tgtttgaaca tacagtcagc ctagtggacc gggtggtccc
     2341 ggtccagcgc gagcagacgc aaagttgcac gtttcggggc acttttatgc agttttgcgt
     2401 ctgctcgccc ggagaactag agcagcttct ccagcgtgat cggcaggtcg gtgactcgtc
     2461 ggcccgtcgc attgaacacc gcattggcga tcgccgcggg cacccccacg atcaccaact
     2521 cgccaagtcc cttcacaccg agcggatcgg cgatggtgtc ctcggcgggc aggtaggtgg
     2581 cgtccagcgc tggcacgtcc gcattgaccg ggaccagata gtccgacatg ttcgcgttga
     2641 cgatgcgtcc gtcgcggtaa tccagctggg tgccctccag tagcgccatc ccgataccgc
     2701 ccaccattcc gccgatagct tggctgtgcg caagcttcgg gttgatgatc ctgcctgcgt
     2761 cgtagcagcc gtagacgcgc cgaacccgca cggtgcccag ggcttcgtcg acggcgactt
     2821 cggcgaacac ggcgccgtag gccgacatcg aatagcgttc gtccgcgtcg tcgggtgacc
     2881 atgtctgttg cgagtcaaga cttccccagc cgcgacgacg caacaagtcc tgatagcttt
     2941 ccccgctggc cggctcgctg gtccggaaca tccgtccgtc tgcgatcgag acgtcctcag
     3001 gccgtagccc attcagcggc gagcccgggt cgagcaccgc agtgcgcaca aaccggtcgc
     3061 gcagcatgtt cgccgttgtg aacaccgcag acccgacgct cgccatcgtc ctcgaacccg
     3121 aatgcgacgg cgctgttgga aaccggctgt ctccgagcgc gaatcgcacc cggttgaatg
     3181 gcagtccgag cgcatccgcg gccacctgcg tcatcgacgt gtatgtgccg gggcccatgt
     3241 cgcttgtccc gcactgaatg tcggcggtgc cgtcggcgtt gatccgggcc agcgccgcac
     3301 actcgctgcg cgaggtgtgg tatacggccg cagccgttcc gatgccgatc agttgattgc
     3361 cgtcccgcgt cgcgcgcggc gtcgggtttc ggcgtgccca gccgaacctg tcggcgccct
     3421 gccggaagca gtcggtcagc cgccgcgtcg agaacggtcg attgtcggtg agatcgcggt
     3481 cgggttcgtt gcgcagccgc aactcaaccg ggtccatccc gagccggaac gccaggtcgt
     3541 ccatcgccga ttccagggcg aaggcgccgg tcgttgcgcc ggggccacgc atgtacgtcg
     3601 gcgggttgat atcaagcggc acaacgcgat acgtcgaccg catattcggt gcaccgtaca
     3661 ggaacctggc tccggtcatc aggttgtcct cgtacgcgtt gtaccgcgag gtctccgtgt
     3721 gcgcctcatg cacgatggcg ccgacgcgcc ccgaccggtc ggcgccgatc gccatccgct
     3781 ggcggctggt cggccggaac ccgatgccgc tgtagtactg ctttcgcgtc agcatcagct
     3841 tgaccgggcg ccgaatctgc ctggccgcga acgccgccag caattggtgg ggccacacgg
     3901 tgcccgcact gccgaacgcg ccgccgacga acggtgagat cacccgaact tgatcgccgg
     3961 gcaccccgaa ggcatcggcg taggacttct gtgccccgct gatgctctgc accttgtccc
     4021 acacggtgag ccggtcgcca tcccaactcg cgaccgtcgc cgggacctcc atggggttgt
     4081 ggttgttgcg catgatcgag tagcgcagat cggtgacgac cgcggcggcc cgcaactcat
     4141 cgtcggcatg gccccgcgca tagtcgcgtg tcctgctgcc cggcttctgc tcggcctggg
     4201 gcgcatcgat attcgtcagc tgcgggccag gacggtactt cacctccact agcgacgcgc
     4261 cgtgcatggc cgcctcgagc gtcgtcgcaa ccaccaccgc gacgggctgg ccgagacagg
     4321 acacctgccg aatgtcatac ggcagcttga ccccgccgaa gtcggtgagg acgcgcaaca
     4381 catcgcggtc gcggacagcg gcagcggggt cgatccgctc caccgcgccg cgcgcgatgg
     4441 tgctgcacac caacgctgcg tacaccaagt ccggcgccgg gttatcggcc gcgtacatcg
     4501 ccttgccggt gaccttgagc cgaccgtcca cccgggtcac tggctggccc gacaccagcg
     4561 ccgcgggcag tggccgcgtc acggcaaccc cgcgatcgtc gccagctgcc gctccaccgt
     4621 gcgcttgatc agctccacct tgaacccgtt ctccgacaac ggctttgcac cttctgcggc
     4681 gttagaggcc gcgtcccgcc acaaggctgc gctcaccggt cgaccgacca gtgcctgttc
     4741 tactgcgggc aaccgccacg gcaccgtccc tacgccgccg acggcgacgc gagcggcgcg
     4801 cactctcccc gcggcgatgt ccagcgccac cgctgccgag gccagcgcaa attcgaagga
     4861 ctcgcggtcg cgcaccttca agtagccaga tcgccgcgct tccggcgtga ctggcacctc
     4921 gatgccggtg atcaacgcgc ccgcggccag gttgtgctcc cgatccggtg tgccgccggg
     4981 ctgacggaag aagtccgcga tccgaatcgg gtgctcgccg tcgccgtccc gcgtgattac
     5041 caacgcgtcc agggcgatca gtgccaccgc gagatcggac gggtgggtgg cgacacaggc
     5101 gtcgctcgtc ccgaggatcg cgtgggtgcg gttgcgaccc ccgatggcgg cgcagccgga
     5161 gccgggtgtg cgtcggttgc aggcggctga cacatcgcgg aagtacaggc agcgcgggcg
     5221 ctgcatcagg ttgccgccga tcgatgccat gtttcggatc tgcgcggacg ccgaaagctc
     5281 caatgcctgt gcaatcactg ggaactggct gcgcacaccc cgatgagcag ccaactccga
     5341 catccgcacc agcgaaccga cccgcaacct cgacgcttgc aggtcgatgt tgcgatacgg
     5401 cagtgcattg atgtcgatga cggaagcggg acgctcgacc gtctcgcgca tcaagtcgat
     5461 cagggtcgtg cccccggcga tgtagcggcc tccggatgcg gccgcggtca atgcggcggc
     5521 ttcgtcgaag gccttggtga actggaaggg gaacacggtc ggctatgtgc tgcgcgccgc
     5581 gttcgcgacc gcagtaacga tgttggtgta ggcgccgcac cggcagatgt tgccgctcat
     5641 ccactcgcgg atctcggcag gggatcgggt gtgaccctcg tcgatacagc cgacgccgga
     5701 catgatctgc ccggccgtgc aatagccgca ttgcaacgcg
//
LOCUS       DASRJA010000539        14628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2880547,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000539 DASRJA010000000
VERSION     DASRJA010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14628
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2880547"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFC01_23785"
     CDS             <1..427
                     /locus_tag="VFC01_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82671.1"
                     /translation="FTPPTGLFTVSVPEGWARSTDGAATIFTDKLNTVRIETRARATA
                     PNTMSVSVDELPAIASSTPGYRPGTVTAVQRKAGQVMLITYQGASPPNPVTGKTGTDA
                     IERYEFWRDGREVILTLSGPVGADNVDPWRTVTDSLQWR"
     gene            391..1101
                     /locus_tag="VFC01_23790"
     CDS             391..1101
                     /locus_tag="VFC01_23790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007296116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN82672.1"
                     /translation="MADRHRFPAVAVTAEPATPPALEARSLYRFYRAGDEETLALQGV
                     SLKLEAGEFVAVAGPSGSGKSTLLACLAGMDNPDGGSVRIAGQRISHRPEPERARIRA
                     RHIGMLFQNANLLDHLTVAQNLSLVRSLAGRAHRADRPDLLSLLGLSERAAAYPGQLS
                     GGELARAGLAIALANQPAALLADEPTGELDSVTESRVLDLLSVAAAGGTAILVASHSP
                     AVTAAAARVIRLDDGRVC"
     gene            1101..1829
                     /locus_tag="VFC01_23795"
     CDS             1101..1829
                     /locus_tag="VFC01_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN82673.1"
                     /translation="MTRSRGEQEAVIDATAKPPAVVECTNVSRIFGSGPTAVHAVRDA
                     TCRIPPWARIAVTGPSGSGKSTLLHLIAGLDAPTAGSVRWPAPEDSPRATAIGMVFQG
                     PSLLPSLDVTENVALPMLFAGQAHDAAVSHAHAALDLLGIGDLAAKLPEELSGGQSQR
                     VAIARVLAARPALILADEPTGQLDHHHADKVITVLLDTAISIGAAVIISTHDPAVAAR
                     LPDHWVMRDGHLDTHPNTADSSAP"
     gene            1826..4486
                     /locus_tag="VFC01_23800"
     CDS             1826..4486
                     /locus_tag="VFC01_23800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN82674.1"
                     /translation="MIATWTLGLLRRRRGRLAATAAGIAAAVALLACLGSFLAAAQGS
                     MTARAIRSVTVDWQVEVQPDTQPSTVRGAVRSAPNVQAALPVGYAHSTGFVAQTGGST
                     QTTGPAMVLGIPPNYREQFPGAIRTLVGADNGVLIAQQTAANLHAAPGDTIHIGRAGI
                     APVDVVVDGVVDLPQADSLFQKVSAPVGAQPAAPPDNVLILGQAQWNNVFDPLATLRP
                     DLVSTQIHTALEHSLPPDPGSAYTQVSAAAHNLEARTAGGARVGDNLGAALGAARSDA
                     TYARVLFLFLGLPAAVLAALLTATVVTAGADRRRQDQALLRARGASQSQLIRLAAAEA
                     LVVATIGSAVGLGAARLVGLAAFGSPSLGADPLTAIGWAAASAAAGLAVAAATVLAPA
                     WRDLRDATVTAARAHLRRTRPPRWTRFGLDVAVLAASGTLFWLAGRGGYQIVLAPEGV
                     PTISVSYWAFVAPALLWIGAALLVWRLADLLLGRGWPLVARALRPLARSLSRIAAHSL
                     SRQRAPLAEAIVLLALALAFAASTATLNATYQAQAEVDAQLTNGADVTVTEPPGSAVP
                     PDAGTKIAAIPGVRTVEPLQHRFAYIGADLQDLYGVDPASITTVTALRDNYFQGGTAQ
                     QLMHELAARPDSVLVSAETVKDFQLLPGDAINLRLQDARTHQLTTVGFHYIGVVTEFP
                     TAPKDSFFVANAGYIAQRTGSNAVGAFLVDTGGRDTTAVAARIRTAVGTTATVTDIAA
                     TRGTVGSSLTAVNLSGLTRIELGFGLVLAAASGALVLALGLTERRRSFVIAHALGATR
                     RHLRSFVLAETAILVACGLAAGAILGAALSQMLVRMLTGIFDPPPTALSTPWTYLAAI
                     AAVLIVAISAVSGVTVRLARRSPLTVLGEL"
     gene            5739..5954
                     /locus_tag="VFC01_23805"
     CDS             5739..5954
                     /locus_tag="VFC01_23805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82675.1"
                     /translation="MGEPRPSWRIATFITEHEAEAPLGYPEIDHLSSPLRAASVRAGA
                     ASRQCVGGQWISKAKSAPVAEIVKILT"
     gene            6014..6691
                     /locus_tag="VFC01_23810"
     CDS             6014..6691
                     /locus_tag="VFC01_23810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN82676.1"
                     /translation="MRLLVVEDEQRLAAGLRKGLEAEGFAVDVVHNGTDGIWMARENP
                     FDAIILDVMLPGANGYQVCRTLRAEGNWTPILMLTAKDGVWDEVEGLDTGADDYLAKP
                     FSYAVLVARLRALRRRGARARPTVLEAGDLRLDPAARRVWLGGEQIALTRREFAILEY
                     LLHHRDEVMSKRDILDHVWDFDFDGDPNIVEVYVRRLRTKLQRPGDGALIETVRGAGY
                     RLVVARD"
     gene            6684..8021
                     /locus_tag="VFC01_23815"
     CDS             6684..8021
                     /locus_tag="VFC01_23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN82677.1"
                     /translation="MIKRLATIRAGTTAVAIVIVAIALVAGAIALLTVLRGTLIDEVK
                     DAAGAQALEVARQLDSGQPPILEVAPSDEQLIQVLGPDGSVLASSRNVAGEAAVARLH
                     PGESEQVLTPLDSEEFMAVAEGAQTSDGPRTVLVARALIGVFETTAVVTKVLLIGLPL
                     LLVVVAVTTWVMVGRALAPVDAIRREVDEISAAQLHRRVPQPDADDEIGRLAATMNRM
                     LERLESARNSQRRFVSDASHELRSPITTIRQYAEVALAHPDRMTAQELADVVLAEQHR
                     MQGLVEDLLLLARADEHLPLAREPVDLDDLAFEEGHRLRATTSKRVDASGVGAARVQG
                     DADALRRVFRNLGENAARHASSRVDIALADLGDDVVLTVDDDGPGIPESERERVVQRF
                     VRLDEARSRDDGGSGLGLSIVEEVVRAHGGSMSITQSTLGGARIQVMLPADEG"
     gene            8066..8398
                     /locus_tag="VFC01_23820"
     CDS             8066..8398
                     /locus_tag="VFC01_23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82678.1"
                     /translation="MNIKSVGIVTAVGAAAVAIGTGIAAAQPDDGEGTPITGDALTKA
                     SQAALAFTGGGTVTGSEVGDEESYYEVEVTLADGSQTDVQLDPNFLIVGSKTDGPGAD
                     TEPDGDGN"
     gene            8401..9762
                     /locus_tag="VFC01_23825"
     CDS             8401..9762
                     /locus_tag="VFC01_23825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82679.1"
                     /translation="MHASGRRLVTLAICATIVSALSVACSGGPAPVAADCIKVVSESG
                     ERTDECLPVAPDSERIDLATPKFSHPTPITNPLHPTSRVEQVIFGGHVDSKPFRTEVT
                     LLPETKPTPYRGTTIDTALIQYVAYLDGRIQEVAIDRYAQADDGSVWYFGEDYSAFED
                     GKVTDTEGTWVVDDKTPAALIMPAHPRVGDVYRPENAPEVVFEEVRVDKVDMNVPGPS
                     GNISGAMEVNELHMDGTREGKIFAPGYGEFSTGDPGGDLEAVSLASPTDSRPDPAPAE
                     FGALSAAVRAVFEAAADSDAEHAKQAATTLDQAWDAALTKGVPPQSQSQMKRDMGELA
                     TAVDVKDWRSAQSAALRVVQNELDLRLLYQRVIDVDVARMALWADQLQIDVAADDGGA
                     VLADVAALERVWERTRHGVDSAGAVDAALQQLRKVADAEDLPAVDGAAATLKQALAGL
                     HAR"
     gene            10005..11012
                     /locus_tag="VFC01_23830"
     CDS             10005..11012
                     /locus_tag="VFC01_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN82680.1"
                     /translation="MAVPLLGPMTLSGFGHAWYFVFLLVVLGLIGLYIIVQLARKKRL
                     LRFANMELLESVAPKRPTRWRHLPTMLLVLSLLLLTVAMAGPTHDVRIPRNRAVVMLA
                     IDVSQSMRATDVPPNRLAAAQDAAKQFADELTPGINLGLIAYAGTATVLVSPTTNREA
                     TKRAVDKLQLADRTATGEGIFTALQAIATVGAVIGGGDAPPPARIVLLSDGKETVPTN
                     PDNPKGCYTAARTAKDQGVPISTISFGTPYGYVEINGQRQPVPVDDEMLKKIAELSGG
                     QAFNASSLVELKNVYVSLQQQIGYETIRGDASAGWLRLGALVLAASALAAMLINRRLP
                     T"
     gene            complement(11112..11285)
                     /locus_tag="VFC01_23835"
                     /pseudo
     CDS             complement(11112..11285)
                     /locus_tag="VFC01_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007238825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
     gene            complement(11530..12624)
                     /locus_tag="VFC01_23840"
     CDS             complement(11530..12624)
                     /locus_tag="VFC01_23840"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN82681.1"
                     /translation="MAVALMLGALAVAFGTQVLAFAATYRTAKTTDAGAALGSDMRLT
                     PADPTNQLPPLGPGIAGVSPFRLVPAQAGSDRKTIMAIDLSTYPDVSTMAPRILEGQG
                     PDALGSDPRGVLLLKELATDFEVGPGDTLPITIYPDDFEKSTNLKLHVLGVYGSFPPT
                     SPPPDQPAELVMSTAAIPRTVPAPPDFYLARVSAGRSADAVAAELRGTLSNQFGVTAV
                     GNPFQRGLTALNLAGLGRLEALGAALIAAVGVAVLGVFLVLERRREFAILRAVGADAA
                     QVIAGPAAEGVLVVLGSLLIGVPVGLGLALLEVRVLSLFFALPPPLLTIPTGALVVFI
                     VFMVMASTIAMAAALAAVTRVRPASVLREP"
     gene            complement(12711..14198)
                     /locus_tag="VFC01_23845"
     CDS             complement(12711..14198)
                     /locus_tag="VFC01_23845"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN82682.1"
                     /translation="MIRWLTARTIRRAPRRVVLGTLGVAFPVAMLAATILFVDDADRA
                     MTRVALEPVQIEMRAMGRSLDVDMTSVSHDLGEVPAVARSEPFAATNVVVETPGAGKW
                     TARLIAVDPSYFAGRPWLRVVRGKPGGGALLDEALRNTPGFASAATVSIALPGDAPKL
                     SLSLPVTGAVDLRQATTWFSIPYGDVQGDVVAVPRSIVIDFSTFARDILPVLQEWATH
                     GGISPVFDPGSTDLPPVSLESHVTVDHAAYPPNPSRATAWSGQLRSLLERRAAGSILV
                     ADNAAETLVTAQSDATNAKILFLLLGIPGVLAAGALGLAAGSALAEANRREEALLRLR
                     GATSGQVLRLAAADAAVVGLAGSVVGLLVAAATVSLVTGGPVWRGVAGGELATTAILA
                     LAVGAATTVIRLIRLRSTERRSDVAVERHLLERGWTPMWKRAYLDLIFIVLGLGILAS
                     TTCPAGCRRPRSKARPWRCRSMSYWHPSFCGSGSPSWRSGSCWRS"
     gene            complement(14195..>14628)
                     /locus_tag="VFC01_23850"
     CDS             complement(14195..>14628)
                     /locus_tag="VFC01_23850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN82683.1"
                     /translation="ARFRRRTCGFIVQGLALLPQATGAENVELPLLLDGIEPAARDRR
                     VAGALERVGLAQHSVKLTDQLSGGQQQRVAIARALVAEPAVVLADEPTGSLDSVTAQD
                     IVALLVAAARERGAAVVMVTHDPTVAAHADRVVAMHSGRIR"
BASE COUNT         2453 a         5037 c         4873 g         2265 t
ORIGIN      
        1 gttcaccccg cccaccggcc tgttcactgt gtcggtgccc gaagggtggg cccgcagcac
       61 cgacggcgcc gcgaccatct tcaccgacaa gttgaacacc gtgcgtatcg agacacgcgc
      121 tcgcgccacc gcccctaata ccatgtcggt cagtgtcgac gaattaccgg ccatcgcgtc
      181 gtccacccct ggttaccgtc ccgggacggt aactgcggtg caacgcaagg ccggtcaggt
      241 catgctgatc acctaccagg gcgcctcacc cccgaatccg gtcaccggca aaaccgggac
      301 cgacgcgatc gagcgctacg agttctggcg cgacggccgc gaagtgatcc tgaccctgtc
      361 cggcccggtc ggcgccgaca acgtcgaccc gtggcggacc gtcaccgatt ccctgcagtg
      421 gcggtaacag cagagccggc gacaccgccg gcgctggaag cccggtcgct gtaccggttc
      481 taccgtgccg gcgatgagga aacccttgcg ctacaagggg tgtcgctgaa gctggaggct
      541 ggcgagttcg tcgccgtcgc cggaccctcc gggtcgggca agtcgacgct gctggcatgt
      601 ctggccggca tggacaatcc cgacggcggc agcgtgcgca tcgccggcca gcggatcagc
      661 caccgcccgg agcccgaacg ggcccggatc cgggcccgtc acatcggcat gctgttccaa
      721 aacgcgaacc tgctcgacca cctcaccgta gcgcagaacc tgtcgctggt gcgttccctg
      781 gccggacgcg cccaccgggc ggatcggccc gacctactga gcctgctcgg cctctccgaa
      841 cgggccgctg cctatcccgg ccagctctcc ggaggagaac tcgcccgcgc cggactggca
      901 atcgcgcttg ccaaccagcc cgccgcgctg ctcgccgacg aacccaccgg tgaactggac
      961 agcgtcaccg aatcacgggt actggacctg ctgtccgtcg ccgcggccgg cggcaccgcg
     1021 atcctggtcg ccagccacag ccccgcggtc acggccgccg cggcacgcgt catacgcctc
     1081 gacgacggac gggtgtgctg atgaccagaa gtcgcggcga gcaggaagcc gtcatcgacg
     1141 ccaccgcgaa accgccggcc gtcgtcgagt gcaccaatgt gagtcgcatt ttcggctccg
     1201 gtcccaccgc ggtgcacgcc gtgcgcgatg cgacctgccg cataccgccg tgggcgcgca
     1261 tcgccgtcac cggaccatcg ggatcgggga aatccaccct gctgcatctc attgccggac
     1321 tcgacgcccc caccgccgga tcggtccgat ggcccgcacc cgaggacagc ccgcgcgcga
     1381 ccgcgatcgg catggtgttt cagggcccga gcctgctgcc cagcctcgac gtcaccgaga
     1441 acgtcgccct cccaatgcta ttcgccgggc aggcccacga cgcggcagtg agccacgcac
     1501 acgcggcact tgacctcctc gggatcggcg acctggccgc caaactgccc gaggagctgt
     1561 cgggaggcca gtcgcagcgg gtcgcgatcg cgcgggtgct cgccgcccgc ccagccctaa
     1621 ttctcgccga tgaacccacc gggcaattgg accatcacca tgccgacaag gtcatcaccg
     1681 tcctgctgga caccgcgatt tcgatcggcg ccgcagtgat catctccacc cacgaccccg
     1741 ccgtggccgc ccggctacct gaccactggg tgatgcgcga cgggcacctc gacacacatc
     1801 ccaacaccgc tgacagcagc gcgccatgat cgcgacctgg acgctcgggc tgctgcggcg
     1861 ccgacgtggc cgactggccg cgaccgccgc cggtatcgct gccgcggtag ccttgctggc
     1921 ctgcctgggc agtttcctgg ccgccgccca gggttcgatg actgcccgcg ccatccgcag
     1981 cgtgaccgtc gactggcagg tcgaagtcca acccgacacc caaccatcga ccgttcgggg
     2041 cgccgtgcgg tccgcaccga acgtccaagc ggcgctaccg gtcggatatg cccactccac
     2101 cggattcgtc gcacagaccg gtggaagcac ccaaacaacc ggcccggcaa tggtattggg
     2161 catccccccg aactaccgcg agcagtttcc cggcgccatc aggaccctgg tcggcgccga
     2221 caacggagtc ctgattgcac aacaaaccgc cgccaacctg catgcggcgc ccggcgacac
     2281 catccacatc ggacgggccg gcatcgcgcc ggtcgacgtc gtcgtcgacg gagtcgtcga
     2341 ccttccccag gccgactcgc tgttccagaa ggtcagcgca cccgtcggag cccaaccggc
     2401 cgcaccgccg gacaacgtgc tgatcctcgg ccaagcccag tggaacaacg tgttcgaccc
     2461 gctggccacg ctgcggcctg acctggtcag cacccagatc cacaccgccc tcgaacactc
     2521 gctgccgccc gatccgggct cggcctacac ccaggtgagc gccgcggccc acaacctgga
     2581 agcccgcacc gccggcggcg cacgcgtcgg tgacaacctc ggcgccgccc tcggcgccgc
     2641 gcgcagcgac gccacctacg cccgggtgct gttcttgttc ctcggcctgc ccgccgccgt
     2701 cctggccgcc ctgctcaccg cgaccgtcgt taccgccggc gcagaccgtc gacgacaaga
     2761 ccaggcgctg ctgcgcgccc gcggcgcctc ccaatcgcaa ctgattcgcc tggccgcggc
     2821 cgaggcactc gtcgtcgcga ccatcggcag cgccgtcggc ctgggtgccg cccgcctggt
     2881 agggctggcc gcgttcggat cgcccagcct cggtgccgac ccccttaccg ccatcgggtg
     2941 ggcggccgcc tcagccgccg ccggactggc ggtcgcggcc gcaacggtgc tcgcaccggc
     3001 gtggcgtgac ctgcgcgacg ccaccgtcac cgcagcccgc gcccacctgc gccggacccg
     3061 gccgccacgc tggacacgct tcggtcttga cgtcgcggtt ctggccgcct ccggaacgct
     3121 gttctggctg gccgggcgcg gtggctacca gatcgtgctc gcgcccgaag gtgtcccgac
     3181 gatctcggtg tcctactggg ccttcgttgc cccggccctg ctttggatcg gcgccgcgtt
     3241 gctggtgtgg cggctggccg acctgctcct gggccgcggc tggccgctcg tcgcccgcgc
     3301 gttgcgacca ctggcgaggt cgttgtcgag gatcgccgcg cattcgctgt cacggcagcg
     3361 ggcgccactg gccgaagcga tcgtgctctt ggcgctcgcg ctggccttcg cggcgtccac
     3421 cgcgacgctc aacgccacct accaggccca ggccgaagtc gacgcgcaac tcaccaacgg
     3481 tgccgacgtc accgtcaccg aacctccggg cagcgccgtg ccgccagacg cgggcacgaa
     3541 aatcgccgca ataccgggag tccgtacggt cgaaccgctc cagcaccgtt tcgcctacat
     3601 cggagccgac ctgcaggacc tctacggcgt cgacccggcc agcatcacca ctgtcaccgc
     3661 gttgcgcgac aactactttc agggcggcac tgcgcagcag ttgatgcacg agctggctgc
     3721 gcggcccgac tcggtgttgg tcagcgccga aacagtcaaa gacttccaac tccttcccgg
     3781 cgacgccatc aacctgcggc tccaggacgc gcgcacccac caactcacca ctgtcggatt
     3841 ccactacatc ggggtcgtca ccgaattccc gaccgcaccc aaagacagct tcttcgtcgc
     3901 caacgccggc tacatcgccc aacgcaccgg cagcaacgcc gtcggggctt tcctggtcga
     3961 caccggcggc cgcgacacca ccgcagtcgc tgcccggatc cgaaccgcgg tcgggactac
     4021 cgcgacggtc accgatatcg ccgccacccg cggcaccgtc ggatcgagcc tgaccgcggt
     4081 caacctttcc ggtctcaccc gaatcgagct ggggttcgga ctcgtcctag ctgccgcgtc
     4141 aggagccctg gttctcgcgc tgggactcac cgagcggcgg cgcagcttcg tcattgccca
     4201 cgcgctcggc gcgacccgtc gccacctgcg gtcgttcgta ctggccgaaa ccgcgatcct
     4261 cgtcgcgtgc ggcctcgccg ccggggccat cctcggcgcg gccttgtcgc agatgctggt
     4321 gcgcatgctc accgggattt tcgacccgcc gccaacagcg ctgtcgacgc catggaccta
     4381 tctcgctgcc atcgccgccg tgctcatcgt tgcgatcagc gcggtgtcgg gcgtgacggt
     4441 tcgccttgcg cgacgctctc cgctgacggt gctgggcgaa ctgtagttcg caccgcgcct
     4501 ggctgggcgc caaccaaaca gtcggatgtc gggcaaccgc ctgggagcat gctctcgttc
     4561 tcgcatcccg acacctgtca ctcgtcgacg aggcatcgca ccgagtggta ttgcgcaagg
     4621 gtcagggctt cggtgtgttc agcgcagatc ggtgcgcccc atcacggccc tggaggcgct
     4681 cgacgcctgc gacgaagcat tggaggtcta cgcagaccgg gtatatgtcg cacaggatga
     4741 cggaggctag atccggttga acctctccat gctctcgcag gacaaggaaa ccagagttgt
     4801 caggattccc gtcgggttac cggtagatgc tgacgggcag cagaatggcg accgccgaac
     4861 ggatcttgcg aatcatgggg tcgtcgttcc tgcggcggcc gccgcgccgg cgtcgaccga
     4921 tgtggccctg cggtggcgct gcaacatggt cactaacacc acggcgtatc gcccgacgat
     4981 cgcgcaatga gaacgagcaa cgaattcgtc gcgtcgcttc ctcggtgggc atctcgcctc
     5041 ccaccaccga tgttctgtag gccgtgtcgt cgaccgtgaa tcgcactgcg ccgttgttgt
     5101 cggcgtgcca gccgaggccg gcgaagcatc cgaagatcgc ggggacagtt aggcgcgtgc
     5161 tgtcaggagt tcgacgcaac cgatgtcgcg tcgcagcgct tctcagcgtc ctctcaacgg
     5221 tgacttggca tcatcgaggc cggtcggatc accacgccag ttacctgcag gagacggtcc
     5281 ataggtgccc gcatcgccgc agcgatctgc gggctgatcg ccctacttgt gctcggtgtg
     5341 tcatcggcgg ccgtcgatgc accggctcca cagccgagaa tcatcccgtt gtctggctcg
     5401 atgaccctag gcggcacatc gacggtgact actgtcccgc cgtcgccgga aatcgcatcc
     5461 gcgtcgccaa ctgtgaaagc gcctcacaag taggccctca ggtaggccgt acaagtagaa
     5521 gttggtgtct tttattcgtt ggtgctgcgc gtgactgagt cagagcgctt cgcgacgccg
     5581 tgagagtgac atcatcgatt catccgtgtc gcccgcctta taccgcttcg acacctatgt
     5641 ggctcgcgtt ggtcagctgt cgacgagagg cggcgggtga acgctgagca ccaatgcgcg
     5701 gtcaacgtga ctgggctttt gtttgcgcgg caacggcttt gggagagccc agaccgtcgt
     5761 ggcgaatcgc gacgttcatc accgagcacg aagccgaggc gccgttgggg tatccggaga
     5821 tcgaccatct gagcagtccg ctgcgggcgg cgtcggtgcg cgcgggcgcc gcaagccgtc
     5881 aatgtgtggg cgggcagtgg atttcaaagg ccaagtccgc gccggtcgcc gagatcgtca
     5941 agatcctgac gtaaccgcac actcgcgaat ttcagccgcc agtcagtttc gggctgcgat
     6001 catggcagcc acgatgcgtc tactggtggt cgaagacgag cagcgcctgg ccgccgggct
     6061 gcggaaaggg ctcgaggccg aaggtttcgc cgtcgatgtc gtgcacaacg gcaccgacgg
     6121 catctggatg gcgcgcgaaa acccgttcga cgcgatcatt ctcgacgtca tgctgcccgg
     6181 cgccaacggc tatcaggtgt gccggacgtt gcgcgccgaa ggcaactgga ccccgatcct
     6241 gatgctgacg gccaaggacg gcgtgtggga cgaagtcgag ggactcgaca ccggagccga
     6301 tgactacctg gccaagccgt tttcgtacgc ggtgctggtc gcgcggctgc gggcactgcg
     6361 ccgccgaggc gcgcgcgcca ggccgacggt gctcgaggcg ggggacttgc ggcttgaccc
     6421 cgcggcgcgc cgggtgtggc ttggaggcga gcagatcgcg ctcacccgcc gcgagttcgc
     6481 gatcctcgag tacctgctgc accatcgcga cgaggtgatg tcgaagcgcg acattctgga
     6541 ccacgtttgg gatttcgact tcgacggcga tcccaacatc gtcgaggtat acgtgcgcag
     6601 actccgcacc aagttgcagc gtccgggcga cggtgcactg atcgagaccg ttcggggcgc
     6661 cgggtaccgg ctggtcgtcg cccgtgatta aacgcctcgc cacgatccgg gcgggcacca
     6721 ctgccgtcgc gatcgtaata gtcgccattg cacttgttgc tggcgccatc gcgcttctca
     6781 ctgtgctgcg cggcacactg atagacgagg tcaaggatgc tgcgggtgca caggcgctcg
     6841 aggtggcgcg ccaactcgat tccggccagc cgccgatctt ggaggttgcc cccagcgacg
     6901 agcagttgat tcaggtgctc ggcccggatg gctccgtgct cgcgtcaagc cgcaatgtcg
     6961 ccggtgaagc cgcagtggcg cgattgcacc caggcgagtc cgagcaggtc ctcactccgc
     7021 tcgacagcga agagttcatg gcggtcgcgg agggcgcgca aacctccgac gggccacgca
     7081 cggtgttggt ggcgagggcg ttgatcggtg tcttcgagac caccgcggtg gtcaccaagg
     7141 tgctgttgat cggattgccg ttgctgttgg ttgtggtggc ggtcacgacc tgggtgatgg
     7201 tcggccgagc cctcgccccg gtcgacgcga tacgtcgcga ggtggacgag atctcggctg
     7261 cacagctgca tcgccgtgtg ccgcagccgg atgccgacga cgagatcggc cgattggccg
     7321 cgaccatgaa ccgcatgctg gagcggctgg aaagcgcgcg aaacagtcag cgccgcttcg
     7381 tatccgacgc gtcgcacgag ttgcgctcgc ccatcaccac catccgccag tacgccgagg
     7441 ttgccctcgc gcaccccgac cggatgacgg cccaagagct cgccgatgtg gtgctggccg
     7501 aacaacaccg gatgcagggc ctggtcgagg acctgttgtt gctggcgcgc gcggacgagc
     7561 acctgccgct ggcgcgcgag cccgtcgacc tcgacgatct ggcgttcgag gaagggcatc
     7621 ggctccgcgc gacgacctcg aagcgggtgg acgcctctgg ggtgggcgcg gcacgggtgc
     7681 aaggcgatgc cgacgcgctg cgcagggtgt tccgcaacct cggcgagaac gcggcccggc
     7741 acgcgtcgag ccgggtcgac atcgctctcg ccgatctcgg cgacgatgtg gtgctgaccg
     7801 tcgacgatga cgggccgggc atccccgaat ccgagcgcga gcgggtggtg cagcgcttcg
     7861 ttcgcctcga cgaagcccgc tcgcgcgatg acggcggcag tggcctcggc ctgtcgatcg
     7921 tcgaagaggt ggtcagggct cacggtggct cgatgtcgat tacgcagtcc accctcggcg
     7981 gtgcccgcat ccaggtgatg ctgcctgccg atgagggttg aagctttcag gttgctgtca
     8041 ggtagttcgc ggcagcctgt cgaccatgaa tatcaagagc gttggaatcg tgacggctgt
     8101 cggtgcggcg gcggtcgcca tcgggaccgg aatcgcggca gcacaacccg acgacggtga
     8161 gggcacgccc atcaccggtg acgccctgac gaaggccagt caggccgcgc tggcgttcac
     8221 cggcggcgga accgtcaccg gcagcgaggt cggcgacgag gagagctact acgaggtgga
     8281 ggttaccctg gccgacggca gccagaccga cgtccaactc gacccgaact tcctcatcgt
     8341 gggcagcaag accgacgggc caggcgcgga caccgaaccc gacggcgacg gcaactagca
     8401 atgcatgcca gcggtcgccg gctcgtcacg ctagcgatct gcgcgacgat cgtctccgca
     8461 ctcagcgtcg cgtgcagcgg cggtccggca ccggtggccg cggactgcat caaggtcgtc
     8521 tctgaatccg gcgagcgcac cgacgagtgc ctgccggtgg cgccggacag cgagcgcatc
     8581 gatctggcca ccccgaaatt cagccacccg acaccgatca cgaacccgct gcatccgacc
     8641 agtcgtgtcg agcaggtcat cttcgggggc cacgtggaca gcaagccgtt ccgcaccgag
     8701 gtgaccctgc tgccggagac caagccgacg ccctatcgcg gcacgactat cgacacagcg
     8761 ctcatccagt acgtcgcgta cctggacggc cggatccaag aggtggccat cgaccggtac
     8821 gcgcaggccg acgatgggtc ggtgtggtac ttcggtgagg actattcggc tttcgaggac
     8881 ggcaaggtca ccgacaccga aggaacttgg gtggtcgatg acaagacacc ggcggcgctg
     8941 atcatgccgg cgcatccgcg cgtgggtgac gtgtaccgcc cagagaacgc accggaggtc
     9001 gtgttcgagg aggtacgcgt cgacaaggtc gatatgaacg tgcccggtcc aagcggaaac
     9061 atcagcggcg caatggaagt caatgagctg cacatggacg gcacgcggga gggaaagatc
     9121 ttcgctcctg gctacggcga gttctccacc ggagacccgg gcggtgacct ggaagcggtg
     9181 tcgctcgcat cgccgacgga cagcaggccg gatccggcac cggccgaatt cggcgcgctc
     9241 tcggccgcgg ttcgagcggt ctttgaggcc gccgccgatt ccgacgccga acacgcaaag
     9301 caagcagcga cgacgttgga tcaagcgtgg gatgcggcgc ttaccaaggg cgttccgccg
     9361 cagtcgcagt cgcagatgaa gagggacatg ggcgagctgg ccacggcggt agatgtcaag
     9421 gattggaggt ccgcgcagtc ggctgcgttg cgggtcgtcc agaacgagct cgacctgcgg
     9481 ctcctgtacc agcgagtgat cgacgtcgac gtggcccgga tggcgttgtg ggcagaccag
     9541 cttcagatcg atgtggcagc cgacgacgga ggcgcggtgc tggccgatgt ggctgcgctg
     9601 gaacgtgttt gggagcgcac ccgccacggc gtcgactccg ccggagcggt ggacgccgcg
     9661 ctgcagcagt tgcgcaaggt ggccgacgct gaagacctgc ccgcggtcga cggcgccgcg
     9721 gccacactca agcaggcact ggcgggactg cacgcccgct agcgctgagt gcacagcgct
     9781 tcccgttgcg tcctcagcgt tcccacagcg ccaaagggag acagtgtggt cctcggcgcc
     9841 cattttccgg cttggccaaa tgtgaaggag aattgatgaa caaccgccac gtgatcgccg
     9901 tcgcggccac cgttgcgatt gtttttggaa gttggccctg acactccgtc gtcgcatggg
     9961 ctcgatcaca ttgtcacctc gtatgtccct cggtgcgacc tgatatggca gtgccgttgt
    10021 tgggcccgat gacgttgtcg ggtttcggac acgcgtggta cttcgtcttc ctgctcgtcg
    10081 tcctcggttt gatcgggctg tacatcatcg tccagctggc gcgcaagaag cggctcctgc
    10141 ggttcgcgaa catggagctg ctggaaagcg tcgctccaaa gcggccgacc cggtggcggc
    10201 atctgccgac aatgctgttg gtgctgtccc ttttactgct caccgtcgcg atggccgggc
    10261 ccacgcacga cgttcggata ccccgcaacc gcgccgtggt catgctggcg atcgacgtct
    10321 cacagtccat gcgcgcgacc gacgtgccgc ccaatcggct cgctgccgcg caggacgccg
    10381 ccaagcaatt tgccgatgag ctgacgccgg gtatcaacct cggattgatc gcctacgccg
    10441 gtactgcgac cgtgctggtg tcgccgacga ccaaccgcga agccaccaag cgcgcggtcg
    10501 acaagctgca gctggccgac cgcaccgcca ccggcgaggg catcttcacc gcgctgcagg
    10561 ccatcgccac cgtcggcgcg gtgatcggtg gcggcgacgc cccgcccccg gcgcggatcg
    10621 tgctcctgtc cgatggtaag gaaacggtgc cgacgaaccc cgacaatccg aagggctgct
    10681 acaccgcggc ccgcaccgcc aaggaccagg gcgtgcctat ctcgaccatc tccttcggca
    10741 cgccgtacgg ctacgtcgag atcaacggcc agcgccagcc ggtgcccgtc gacgacgaaa
    10801 tgctgaagaa gatcgccgag ctgtccggcg gacaggcgtt caacgcgtcc agcttggtcg
    10861 agttgaagaa tgtctacgtc tcgctgcagc agcagatcgg ttacgagacc atccgcggcg
    10921 acgccagtgc cggctggctg cggctgggcg cgctggtgct ggccgcctcg gcgctggccg
    10981 ctatgctgat caaccgccgg ctgcccacct gagcggcact tctgggtgtc cgatgacgaa
    11041 gggcgctgcg ctcacaggga atccgcgacc gacatttgcc tgccacgccg cagctaaatg
    11101 gcgaacggca gcagcaatgc ccgcggaatc aggccgagga cgaagccgat gttcgggatg
    11161 tagttggtga tgaacgacag caagccccac aggattggta acgggatccc cttcagccac
    11221 tgcgccccgg catctaaggc cgcaacaatc aagccgaaaa ccgatgacac caagaggtag
    11281 ctgcgaagat cgaccgcgcg acgagatcga tccgccgact ctccggtgcc agcctgctga
    11341 gcgtccggtt gttcaacccc cagcgacggg tggggaatcg tcgaagaggc cgagggcgcg
    11401 gcgcctggag gcgagtggac tcgggggacg ttcccgagcc caggctgcac ccgccgccca
    11461 cgagtactgc agacggcgcg atgccgacga gcgccgccaa cggttggcga gtggcaccat
    11521 gagttatcgc tagggctcgc gtagtaccga tgccggccgc acccgggtga ctgcggcgag
    11581 cgctgcggcc atggcgatgg tcgacgccat gaccatgaac acgatgaaca cgacgagggc
    11641 acccgtgggg attgtgagca gcggtggggg gagtgcgaag aagagcgaga gcacccggac
    11701 ctcgagcagt gcgagcccca gcccgaccgg aacgccgatc agcaagctcc ccagcactac
    11761 gagcacgcct tcggcagccg gacccgcgat gacctgggcc gcgtcggcgc ccacggcgcg
    11821 aaggatcgcg aactcgcggc ggcgttcgag cacgaggaat acgccaagca cggctactcc
    11881 gacggcggcg atcagcgcgg ctccgagcgc ttcgaggcga ccgagccccg cgaggttcaa
    11941 tgcggtgagg cctcgctgga acgggtttcc gaccgctgtc acgccgaatt gattggacag
    12001 agttccgcgg agttcggcgg ccaccgcgtc ggccgatcgc ccggcagaga cgcgcgcgag
    12061 gtagaagtct ggcggcgccg gcaccgtccg cggtatcgcg gccgtcgaca tgaccagctc
    12121 ggccggctgg tcgggcggcg ggctcgtcgg cggaaacgag ccgtagacgc cgagcacgtg
    12181 gagcttcagg ttggtgctct tttcgaaatc gtccggatag atggtgatcg gcagtgtgtc
    12241 accgggtccg acctcgaaat cggtggcgag ctctttgagc aacaggactc ctcgcgggtc
    12301 gctgccgagc gcgtcggggc cctgaccctc caggatccgc ggtgccatcg tggacacgtc
    12361 gggataggtg gacaggtcga tcgccatgat tgtcttgcgg tcgcttcccg cttgcgcggg
    12421 caccaggcgg aaggggctga cgccggcgat cccgggaccg aggggcggta gctggttcgt
    12481 cgggtcggcc ggggtgaggc gcatgtcgga gcccaaggcc gccccggcgt cagtcgtttt
    12541 cgcggtgcga taggtcgccg cgaacgccaa cacctgggtg ccgaacgcca ccgccaacgc
    12601 tccgagcatg agggcgacgg ccatccgcgc gggacggcgg cccgtccatc gggtggcagt
    12661 tgcgcgccag gaaggcaacg gcttcgaccg tccccgctcc gcccgtcgac tcaagatcgc
    12721 cagcacgacc cggaccgcca ggaaggtgac cccgagccac agaaagatgg gtgccagtag
    12781 gacatagaac gacagcgcca gggacgtgcc ttcgatcggg gtctgcgaca gcccgccgga
    12841 caggtggttg atgcgaggat gcccagcccg aggacgatga agatcagatc gaggtaggcc
    12901 cgtttccaca tcggggtcca gccccgctcg agcagatggc gttctacggc gacgtctgac
    12961 cggcgttctg tgcttctgag ccggatgagc cggatgaccg tggtcgctgc gccgacggca
    13021 agcgccagga ttgccgtggt ggcgagttct ccccctgcaa ccccgcgcca cacgggtccg
    13081 ccggtcacca aactgacggt ggctgctgcg acgagaagtc cgacaaccga ccccgcgagt
    13141 ccgacgaccg cagcgtcggc cgcggccagg cgaagaacct gcccactcgt ggcgccccgc
    13201 agccggagca gtgcctcctc ccgccggttg gcctcggcga gggcagaccc ggcggcgaga
    13261 cccagtgcac cggccgcgag taccccgggg atccccagca gcaggaacag gatcttcgcg
    13321 ttggtggcgt cggattgggc agtcaccaac gtctcggcgg cgttgtcggc aaccaggatc
    13381 gagcccgccg cccggcgctc cagcagcgat cgaagctgcc ccgaccatgc ggtcgcgcga
    13441 ctcgggttcg gcggataggc ggcgtggtcc acggtcacgt gggattcgag gcttaccggc
    13501 ggcagatcgg tggaaccggg atcgaacacc ggggagatac cgccgtgggt ggcccactcc
    13561 tgcagcacgg gcaggatgtc gcgcgcaaag gtgctgaagt cgatgacgat tgagcgggga
    13621 acggcgacca catcgccctg cacgtcgccg taggggatgg agaaccatgt tgttgcctgg
    13681 cgaagatcga ccgccccggt gaccgggagg ctgagggaaa gcttgggggc gtcgccgggc
    13741 aatgcgatcg aaaccgttgc ggccgaggcg aatccgggag tgttgcgaag cgcttcatcc
    13801 agcagcgcgc cgccgcccgg cttgccgcgc acgacacgga gccagggtcg accggcgaag
    13861 taggacgggt cgacggcgat caggcgggcg gtccacttcc cggcaccagg ggtttccacc
    13921 accacgttgg tcgcagcgaa gggctcggag cgggcgaccg caggcacctc acccaggtcg
    13981 tggctgacgg acgtcatgtc gacgtcgagc gacctgccca tcgcccgcat ctcgatctgc
    14041 accggttcca atgccacccg cgtcatggca cggtcggcgt catcgacgaa aaggatcgtc
    14101 gcggccagca tcgcgaccgg aaacgcaacc cccagcgtgc cgaggacgac gcgtctcggc
    14161 gcacgccgaa tcgtgcgcgc cgtgagccag cggatcaccg gatccgcccc gagtgcatgg
    14221 ccaccacgcg atcggcgtga gccgcgacgg tgggatcgtg cgtgaccatg accaccgccg
    14281 ccccccgctc gcgcgcggcg gcaacgagca gcgcgacgat gtcctgggcg gtcaccgagt
    14341 ccaggcttcc ggtgggctcg tcggccagaa cgaccgcggg ttcggccacc agggctctcg
    14401 cgatcgccac ccgttgctgc tggccgcctg agagttggtc ggtcagcttc accgagtgct
    14461 gcgcgagccc cactcgttcg agggccccgg ccacccggcg atcccgtgcc gccggctcga
    14521 ttccgtcgag cagcaacggc aattcgacgt tctcggcccc cgtcgcctgc ggaagaagcg
    14581 ccagaccttg aacgatgaaa ccgcatgttc ggcggcgaaa tcgggcgc
//
LOCUS       DASRJA010000540         3570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3141696,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000540 DASRJA010000000
VERSION     DASRJA010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3141696"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VFC01_23855"
     CDS             <1..1056
                     /locus_tag="VFC01_23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HZN82684.1"
                     /translation="LNSVVALDLITQSWNGLPDLGTARHGMAVAGVGKTVYAIGGSTG
                     PGDGQITSSAEALKLAPRKPQPASAWRSLPDAPTARLMMAWTVLDGKIWVAGGIREGE
                     TLQTVESYDPQTGVWQPQPPLPLPLHHATAAAYRGEVVVIGGASDVIAEASNKVFALR
                     GGTWTELPALQHARAAAAAAVVGDKLVVVGGQNNKQLVPQTEVFDGKSWTQAADLPTP
                     REHLAAASDGVYVYTVGGRELSADKNSAALERYDPASGNWEKLPSMPTPRGSYGAAYI
                     DGRIVAVGGEEPTRVLATVEMYDISSKKWSTVAPLGTPRHGQVVAAVGSALYCIGGAD
                     RPSHEGAIATVEALDFT"
     gene            complement(1049..1666)
                     /locus_tag="VFC01_23860"
     CDS             complement(1049..1666)
                     /locus_tag="VFC01_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN82685.1"
                     /translation="MSSHLEVWKPSGRELVPLSGERVTIGKASTNVVSLEHDPTVSRL
                     HAILENHGSAWSIRDVGSRNGTYVNGEKIAAERVLHSGDEVRVGKSRLVYLEARQAGE
                     PAYEETIVPDTAALPPRLTRREFDVLVVLCRPLVSDDPFPEPASVRRMATELYVTEAA
                     VKQHLQNLYDKFSIPTEGDRRVRLANEALRRGAVTIAQLRDSGAT"
     gene            complement(1734..2747)
                     /locus_tag="VFC01_23865"
     CDS             complement(1734..2747)
                     /locus_tag="VFC01_23865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82686.1"
                     /translation="MFATAPTGSPRRVEEETVRLRPGDLPVRPLPTTNTGIEAKTVRM
                     RSADLLTKKLPKAISGMDGKTVRMRTLDLPVVGKVANTVRQRYVDLSKLKAKPPTKWE
                     IARVALVLLLASMPILAIPADVSGLVSQSTSSVAVIVLLAVLGTLVVFAPHRIDMIVG
                     RGLIAGMVATIVYDGARLFAVHVLGLMGDFIPVMGSFVTGEPDTTGSAAVGYVWRYIG
                     DGGGLGVAFFVVAFALGIDRWKNVYAVLASVAFAVFPTWAGLMATVALLPRGEEMMFD
                     LNPATFIITLVGHLIFGFFLGLAFLKAPRGKDKSGWPWPPLSESAPAKRAIRFVKKVT
                     HSP"
     gene            complement(2882..3295)
                     /gene="tsaA"
                     /locus_tag="VFC01_23870"
     CDS             complement(2882..3295)
                     /gene="tsaA"
                     /locus_tag="VFC01_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HZN82687.1"
                     /translation="MSHDLVLRPIGHVESTLTEPQAAPRQGDEGAPDCWLVFDPAVRP
                     ALDGLRPGDNIVVVTWLHLADRTILQVHPRGDTTRPIRGVFATRSQHRPNPLGIHQVT
                     VLAIDDLRLQVQGLEAVNGTPLIDIKSALEPRGQR"
     gene            3353..>3570
                     /locus_tag="VFC01_23875"
     CDS             3353..>3570
                     /locus_tag="VFC01_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HZN82688.1"
                     /translation="MTSAPISDNGPRRAILGQLPRMHRADGSPIRVLLVDDERALTNL
                     VRMALQYEGWDIDVAHDAREAVDKYRANT"
BASE COUNT          644 a         1133 c         1201 g          592 t
ORIGIN      
        1 ctcaatagcg tggtcgcgct cgatctcatc acgcaatcgt ggaatggctt gccggacctg
       61 ggtactgcac gccacggcat ggcggtggcc ggcgtcggga agacggtgta tgcgatcggc
      121 gggtcgaccg gtcccggcga cgggcagatc acgtcgtcgg ccgaagcatt gaagctggca
      181 ccacgcaaac cccaacccgc gtcggcatgg cggtccctac cggatgcgcc gaccgcgagg
      241 ttgatgatgg cgtggacggt tctcgacgga aagatctggg ttgccggcgg gattcgcgag
      301 ggcgaaacgc tgcaaacggt cgagagctat gaccctcaga cgggggtgtg gcaaccgcaa
      361 ccaccgctgc cactaccgct gcatcacgcg acggcggccg cgtaccgcgg ggaggtagtc
      421 gtcatcgggg gcgccagcga cgtcatcgcg gaggcgtcaa acaaggtatt cgcgctacgc
      481 ggaggcacct ggacagaact accggccctc caacatgccc gggctgcggc ggcggcagcg
      541 gtcgtcggtg acaagctcgt ggtcgtcggc gggcagaaca ataagcagct cgtcccacag
      601 accgaagtgt tcgacgggaa atcctggacg caggcagccg atctgccaac cccgcgcgaa
      661 catttggctg cggcgtcgga tggcgtctat gtgtacacgg tcggcgggcg ggaactgtcc
      721 gcagacaaga actcggcggc gttggaacgc tatgacccgg cgtccggaaa ctgggagaag
      781 ctcccgagca tgccgactcc gcgcggcagc tatggcgccg catacatcga cggcaggatc
      841 gtggccgtcg gcggtgagga gccgacgcgc gtactggcca ccgtcgagat gtatgacatc
      901 tccagcaaga agtggagcac ggtcgcgccg ctcgggactc cgcgtcacgg ccaggtggtg
      961 gctgcggtcg gctcggccct gtattgcatt gggggcgccg accggccttc gcatgaaggg
     1021 gccatcgcta ccgtcgaggc gctggacttc acgtagcgcc gctgtcgcgc agctgggcga
     1081 tggtgacggc tccgcgccga agcgcctcgt tggctagccg gaccctgcgg tcgccctctg
     1141 tggggatcga gaacttgtcg tacaggttct gcagatgctg tttgacggcc gcctcggtga
     1201 catacagctc ggtcgccatc cgccgaaccg atgcaggctc gggaaaaggg tcgtcggaga
     1261 ccagggggcg acacaacacc acgagcacat cgaattcgcg ccgagtcaga cgcggcggaa
     1321 gggcggcggt gtcgggaacg atcgtttctt cgtaagccgg ttcgccggct tgccgcgcct
     1381 cgaggtagac caaccgtgac ttgccgacgc gtacctcgtc gccagaatgc agaacccgtt
     1441 ccgctgcgat cttttccccg ttgacgtagg tgccgttgcg actgcccaca tcgcgtatcg
     1501 accaggcaga cccgtggttt tccaagatgg cgtgcaggcg agagactgtc gggtcgtgct
     1561 cgagcgacac cacgttggtt gaggccttac cgatggtcac tcgttcgccg ctcagtggga
     1621 ccagctcccg tcccgacggc ttccagactt ccaggtggga agacatgcag acctcctatc
     1681 tgggcaattt tgacccagat aggaggtctg tggtctgcga acacgcgccc tacctacggc
     1741 gaatgtgtga ctttcttgac gaagcgaatg gcccgcttgg cgggggctga ttccgagagg
     1801 ggtggccacg gccatccgct cttgtccttg ccgcgcggtg ccttcaggaa ggccagcccc
     1861 aggaagaatc cgaagatcag gtgcccgacc agggtgatga tgaacgtcgc cggattcagg
     1921 tcgaacatca tctcctcgcc acggggcagc agggcgacgg ttgccatcag gcccgcccag
     1981 gtggggaaga ccgcgaaggc caccgaggcc agaaccgcgt agacgttctt ccaccgatcg
     2041 atgcccagcg cgaacgcaac gacgaagaat gcgacgccca gaccaccgcc gtcgccgatg
     2101 taacgccaca catagccgac cgccgcactg cccgtggtgt ccggttcgcc ggtgacaaac
     2161 gaccccatca ctgggatgaa atcgcccatc aggcccagca cgtgcacggc gaacagccgg
     2221 gctccgtcgt agacgatggt cgccaccatg cctgcgatca gaccgcgtcc gacgatcatg
     2281 tcgatgcggt gcggcgcgaa cacgacgagc gtccccagca cagccaacag cacgatgacg
     2341 gcgaccgacg aggtgctctg cgagaccagg ccggacacgt ccgcggggat cgccaggatc
     2401 ggcatcgacg ccagcagcag gaccaaggcg acccgcgcga tctcccactt ggtgggcggc
     2461 ttggccttga gcttggacag gtcgacgtag cgctgccgca cggtgttggc gaccttgccg
     2521 acgaccggca ggtcgagcgt gcgcatgcgt actgtcttgc cgtccatgcc gctgatggcc
     2581 ttcggcagct tcttcgtcaa caggtccgcc gagcgcatcc gcaccgtctt ggcctcaata
     2641 ccggtgttcg tggtcggcag cggccggacg ggcaggtcgc cagggcgcag ccgcacagtt
     2701 tcctcttcga ctcggcgtgg ggagccagtc ggtgctgtcg caaacatgag tcttcctaat
     2761 ctcggtccgt cttcgagggg gtacgacatt catcctcgtg atcaggcaac gcttgcgtaa
     2821 ttgctgggaa gccagacttc cgccctcggg gggaaggttt tcctatccgc ccggtaagga
     2881 gtcaccgctg cccgcggggc tcgagtgcgg acttgatgtc gatgagcggc gtcccgttga
     2941 ccgcctccaa gccctgtacc tgcaggcgga gatcgtcgat agccagcaca gtgacctgat
     3001 gaatacccag cgggttgggc cgatgctgcg aacgggtggc gaacaccccg cgaatgggcc
     3061 gggtcgtgtc accacgcgga tgcacctgca ggatggttcg gtcggccagg tgcagccagg
     3121 tgacgacgac gatgttgtcg ccggggcgca ggccgtcgag tgccggccgc acggctgggt
     3181 cgaataccag ccagcagtcg ggcgcgccct cgtcaccctg acgcggagct gcttgcggct
     3241 cggtcagcgt cgattcgaca tgcccgatgg gccgcagcac gaggtcatgg ctcacatttg
     3301 aaatatctat gaggctcgtg acgccccgct gtgtgatcgc aagaatggtc aaatgacgtc
     3361 cgcgccgatc agcgacaatg gtccgcgccg cgccatcctg ggccagctgc cgcggatgca
     3421 ccgcgccgac gggtcgccga tccgggtgct gctcgtcgat gacgagcggg ccctgacgaa
     3481 tcttgtccgg atggcgctgc agtacgaggg ctgggacatc gacgtcgctc acgacgcccg
     3541 ggaggccgtc gacaagtacc gtgccaacac
//
LOCUS       DASRJA010000541        23585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4163552,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000541 DASRJA010000000
VERSION     DASRJA010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 23585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23585
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4163552"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFC01_23880"
     CDS             <1..825
                     /locus_tag="VFC01_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZN82689.1"
                     /translation="LERLVPAGSQQPCLVRVIPGVEAATHASQATGHAGSKFGLLPDD
                     ARDAIARIERSAKLRLDGVHTHVGSQLLNVEQLAQAVEPIAKLGTFDVYDLGGGLGVR
                     YTYDEHPPSLDDYADAMVTQAKALLPEGARIIVEPGRSMVANSACTVYRITTIKRGVV
                     THVAVDGGMGDNLEVSLTGQRFEATIVNRVGGGEMVSVVGRHCESGDQLVDGVPLQQP
                     AIGDLLAVPVTGAYCYTMSNQYNGARRVPVVFARNGTPRLVVRRDTWDDLLIRDVD"
     gene            846..2981
                     /gene="malQ"
                     /locus_tag="VFC01_23885"
     CDS             846..2981
                     /gene="malQ"
                     /locus_tag="VFC01_23885"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZN82690.1"
                     /translation="MTELPDSLVELARRYGVATEFEDWTGRRTAVADSTLIAVLDALG
                     VDAATEDERAAALAAHDRDHWSHSLPPTIVGRSGVASPFWVHVTHGEPAGLWLRLEDG
                     SVRTGLRQLENNRAPYDLDGQLIGEATFELPDDLPLGYHQLHLQVGASDVNTVVIVTP
                     ASLGLPARLGTSRAWGLATQLYSVRSQRSWGIGDLTDLADLAVWSAAQHGAGFVLVNP
                     LHAAAPTPPMEPSPYLPTSRRFINPLYLRVEAIDEFAYVRQRGSIRKARVEVQAHAKK
                     SKRIDRDAVWKAKRAALKSVYRVQRSAGRELAYAAYCHREGRSLDDFATWCALAEEYG
                     NDWHQWPEELQHPAGPAVSEYAAKHAKTVDFHRWLQWQLDDQLTAAQATAVQAGMDLG
                     VMHDLAVGVDPNGADAWALQDVLALGVTAGAPPDEFNQLGQDWSQPPWRPDRLVDQAY
                     EPFRALVNAVLRHAGGVRIDHIIGLFRLWWIPKGAPPTDGTYVRYDHEALIGIVALEA
                     HRAGAVVVGEDLGTVEPWVRDYLRERGLFGTSILWFEADRDGDGGPLPAENWREFCLS
                     SVTTHDLPPTAGYLAGEHVRLREKLGLLTRPAAEELASDQAQQAAWLAELRRVGLLGD
                     DPSVEEVVLALYRYLGRTPSKLLALSLVDAVGDLRTQNQPGTTDEYPNWRVPLRGPDG
                     RKLLLEDVFTDPRAATLAEAMKHIVHPPV"
     gene            3059..3475
                     /locus_tag="VFC01_23890"
     CDS             3059..3475
                     /locus_tag="VFC01_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82691.1"
                     /translation="MKKIVVLSGGLVAAGSAALIGAGVALSQPQPDPSSYNVVGEPYQ
                     KALMILKSQGVKAYFGGSFGSVLPQAQCLVDQQKITSGGRMLLSLDCSQNAADILAAM
                     GPSGGPTVGSNGVTTVTPTPVVPIQGAPGPAAPPPA"
     gene            complement(3472..3906)
                     /locus_tag="VFC01_23895"
     CDS             complement(3472..3906)
                     /locus_tag="VFC01_23895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN82692.1"
                     /translation="MRVQRHCVIDADRDTVWKVVSDPDCYPEFMASLERWEHVTEGPA
                     RKGSRYTVHWKIRSVPIGGVVEVSEFDPAHDLAWIGVSGVSLRGRFRIRDNGDGHTKV
                     IFRLAYESAGNLLGLIADRVAARQVGRNMTETLKNLKQLVEA"
     gene            complement(3938..4771)
                     /locus_tag="VFC01_23900"
     CDS             complement(3938..4771)
                     /locus_tag="VFC01_23900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN82693.1"
                     /translation="MAGLGDVVEWARHQVSSRVPKADLDQRDPDYIREQLPGTWLLAS
                     LYFRADVRGLDRIPAEGPVLLVGNHSGGNVPPDTFVFTLAFCSYFGVERPFYQLAHNL
                     VVSAPPLGWLRKFGTVAANHENARMALESGAALLVYPGGDYEVFRPSWERHKVDFGGR
                     KGYVRLAREAGVPIVPIASVGGQETALFLDRGQWLAKLLMVDKLARLKSVPISLALPW
                     GLNIGDLLGHIPLPAKIVVEVLDPIDVDGDDEVVHKKVVESLQAGLDRLAAERRFPII
                     G"
     gene            4849..5748
                     /locus_tag="VFC01_23905"
     CDS             4849..5748
                     /locus_tag="VFC01_23905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZN82694.1"
                     /translation="MLRPHRFDTEVDPGPVQIQARKVHFNVSGIPLHWIPGHPVASNV
                     ISLLNIVLPAAERWFVLTLNEALPLVKDPHLAEDMRGFIGQEAMHADVHEQVLHEYMV
                     INGIDPKPILDQVEYAFEKVLAPTTSTDPKRRLNHLCDRLWLIAAVEHYTAVMGDFAL
                     NCAWDDHDASPTMVDLFRWHGSEEVEHRMVAHDVAVYFHDSYIDRIRAMSMAVVMIFV
                     MFQRAAWYIVKTDPDTDIGWWKFNRMRMRDSKLGLLPKYRKLFGSNTAMYFRPGYSPE
                     EMGSTAQAVAYLASSPAARAAHL"
     gene            5748..6842
                     /locus_tag="VFC01_23910"
     CDS             5748..6842
                     /locus_tag="VFC01_23910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HZN82695.1"
                     /translation="MGLLERYRQAPPSAFGRLRHDVVMGLADVTLNGLWAISGAIRRM
                     SAPPDTDRTIALTVTDRKVVAHDQDVVTLTLVGEQLPRWHPGAHIDIHLPSGLVRQYS
                     LCGDPATPDTYRIAVRRIPDGGGGSIEMHDLRVGATVTTHGPRNAFPLTVPGYGSPAR
                     RFRFIAGGIGITPILPMLGLAQRLAVDWSMVYAGRSRESLPFLDEVSRFGDRIEVRTD
                     DVSGLPTAAELLGGCPDGTAVYACGPAPMLTAIRSALAGRDDVELHFERFAAPPVVDG
                     KDFSVSVAATGQTVRVGADETLLSALNRAGVHAPYSCQQGFCGTCRTRVVDGAVDHRD
                     TLLTDPERDSGMMLICISRAADGSHLTLDL"
     gene            6864..8243
                     /locus_tag="VFC01_23915"
     CDS             6864..8243
                     /locus_tag="VFC01_23915"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN82696.1"
                     /translation="MPLADGATFAGYTIVRMLGSGGMGEVYLAQHPRLPRRDALKVLP
                     ASVSADSEYRQRFNREADIAATLWHPHIVGVHDRGDFEGQLWISMDYVDGTDASRLLR
                     ERYPSGLPKAEVANIVTAVAEALDYAHERGLLHRDVKPANILLAYPDSGDQRILLADF
                     GIARWADDISGLTETNMTVGTVSYAAPEQLMGQQLDGRADQYALAATAFHLLTGAPPF
                     QSSNPAVVISQHLSASPPAVGAQRPELSALDPVLAKALAKDPKDRFERCADFARAFAH
                     RLDAAPTGDVDTGLASAARPQKRSLLRAGVIVPAVLALLLIAAITVAIMEFRRADRPE
                     TPTATATSPTATTPPVTSPTFTPLPPPPEPVPPPAAVIGANCSPEGSTATTPEGATAY
                     CSTLQSTGATIWSLNPGDVAEPTVTAAPTDEPLPVAEENPVRVCMQQTGRTRLACREM
                     IRRSNLGLP"
     gene            8240..9115
                     /locus_tag="VFC01_23920"
     CDS             8240..9115
                     /locus_tag="VFC01_23920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN82697.1"
                     /translation="MKFAVIAPVAAGVTADPEWMSGFARHVEACGFESIVAVEHTVLM
                     TEYRSVYPYAESGRVELRADCPVPDPLDLLTFLAAHTNRLGLATGVLVLPNHHPVVLA
                     KRVATVDALSGGRIRLCVGMGWLKEEIEACGAEFASRGRRADEQIEVMRLLWEDRPGG
                     ASYSGQFVEFDHATCYPKPTGRVPIHIGGHSRAAARRAGRLGDGFQPLGVGGARLSEL
                     IGIMRDEANKAGRDPDALELSLGHLVTKVDGERADKLAGLGADRIVLAMPPTADLGEA
                     CDVLSACARRLALIP"
     gene            9112..9576
                     /locus_tag="VFC01_23925"
     CDS             9112..9576
                     /locus_tag="VFC01_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN82698.1"
                     /translation="MNLNAYDRLALADLVHLYASAVDDRRFDDVVELFTETAELRLPD
                     PPRSLEPVRHHQGRGGVRQAMAALAAVTRTEHAIVGEVYAPGDDSDYALGRITCIAHH
                     WTADADQITDLVWHLRYDDEYLRTRAGWRIHGRALTINAIETRPVRRLRGQR"
     gene            complement(9533..10018)
                     /locus_tag="VFC01_23930"
     CDS             complement(9533..10018)
                     /locus_tag="VFC01_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZN82699.1"
                     /translation="MPKLSAGLLLYRVTDGGVEVLIGHPGGPFWARKDEGAWSIPKGE
                     YVEGEDPWTVAQREFEEELGKAPPEGPRIDFVPLKQPSGKIITAFAVRGDLDLEGTFS
                     NTFELEWPKGSGNIREYPEIDRVGWFSVTEANEKLLTGQRPLLDRLTAALEGDEPGGS
                     R"
     gene            complement(10018..11037)
                     /locus_tag="VFC01_23935"
     CDS             complement(10018..11037)
                     /locus_tag="VFC01_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZN82700.1"
                     /translation="MPELTNRQILLRRRPTGLVGPDDTELVTNPAPEPADGEALVRTT
                     YVGIDAAARTWLDGQPGYLPALQLGDVIRAAGIGEVVASRCDAYAVGDVVTTLTGFQE
                     YVIIRDDIFTTPIPGETDQLAIMSVYGPTGATAYFGMTDIGKPKPGETVVVSAAAGAT
                     GSVAGQIAKIAGARVVGIAGGPHKCKAVVEDFGFDACIDYKEAGLPAALKEHCPKGVN
                     VYFDNVGGPILDAVLGRLAPKARVVLCGVISSYLTGEHPGPANYVNLLARTASMQGFN
                     ALDEWGRFDEAFASLRQWEADGRLVHRQTIFEGIESCVDALNGLFTGANIGKTLVKLS
                     EPTTA"
     gene            complement(11058..12095)
                     /locus_tag="VFC01_23940"
     CDS             complement(11058..12095)
                     /locus_tag="VFC01_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HZN82701.1"
                     /translation="MSGTTSFTSVPIVDISGLGSPDRQERERVAAELGKAASEVGFFY
                     VGGAGVDDLLFDRMLAATKEFFALPLDEKMRYYIGLSACHRGYVPSGEEGLATGMPEM
                     KEAFDTALDLPADDPDYLAGNPMLGPNTWPDLPGFADAVTAYYEAVLDVGQRLLRAFA
                     VALGEDPDTFSKHATKTPSQLRLIHYPYNPGAVDGEGIGAHTDYECFTLLKPTAPGLE
                     VLNGAGEWIDVPPVPGTFVVNIGDMLELWTNGAYVATSHRVRKVKEERYSFPLFFNVD
                     YHTEVKPLPQFASRDGKQRPALRAGEHLFAQTAQTFAYLRNRVKSGELVLPDGSLETG
                     QFGQQALQKRA"
     gene            complement(12092..12607)
                     /locus_tag="VFC01_23945"
     CDS             complement(12092..12607)
                     /locus_tag="VFC01_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82702.1"
                     /translation="MTVLMTECSGLWRRTLLIAGDGSRDVSTDVAWLQGISAYVDTRG
                     FAGRLGQHGDVFEWQRLIDIEPPGPFPDAGRMRWDAGVLIEVGVHEDYVEHWVRDDDP
                     GVRCWALFLHNAILLRAGAHFGWADGNGVVLHTIGGPQWTTLNPRVNGSDLVANGVRW
                     RIEDSEGDVDL"
     gene            complement(12604..13614)
                     /gene="alc"
                     /locus_tag="VFC01_23950"
     CDS             complement(12604..13614)
                     /gene="alc"
                     /locus_tag="VFC01_23950"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HZN82703.1"
                     /translation="MKVDLASRALGGSVVAASDESFGFKERLVEPSEPAFVPGTYDLR
                     GEVVDGWETRRYVGGGGDWAIVRLGAGGRLRAIDVDTRFFAGNHPTACRIDACVLDVV
                     DDATAAAAQWTTVVETTALKPDSHNVFAVDDPRRYTHVRLNLESDGGVARLRVYGEVI
                     PDPATWAGITVEVSGVEQGGRVEWSSDSFYSDACVLIAPDRPRTMGDGWETKRRRDIG
                     PDTHDAVMISFAVPADLQRIEVDTSRFVFNASREVAVLGSRGVPCGEHGWALVPFDVE
                     VLTRTRIAPDARQVFIVAATDITALRVQAYPDGGIARVRAFGRPTADGIALLESMWQA
                     SA"
     gene            complement(13611..14363)
                     /locus_tag="VFC01_23955"
     CDS             complement(13611..14363)
                     /locus_tag="VFC01_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HZN82704.1"
                     /translation="MRILVLNPNTSESMTAEIAAAAAAVAAEGTEIVARNPRFGATAI
                     DSAAESYLSAVGVMDVVTTMLAVGEFDYDAVILAGFGEHGKDALQEMLPVPVLDIAEA
                     AAHVAHLIGRRFCVVTTLARSIPPIEDRLMLAGLDAHCASVRACGLGTAEVDADPAGA
                     VQAIVDEAARAIAEDGADVICLGCAGMAGVTAVITAKLGVPAIDGVAAAVALAQALVG
                     LGLSTSKAGVYAAGPDNPRSHWPLSTALGLDR"
     gene            complement(14360..15649)
                     /gene="allB"
                     /locus_tag="VFC01_23960"
     CDS             complement(14360..15649)
                     /gene="allB"
                     /locus_tag="VFC01_23960"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HZN82705.1"
                     /translation="MDLVIRTEKAVIDGEVRAAAVAVADGVIRGIGLIGDFSGQESIR
                     LPPSTVLLPGFVDTHVHINEPGTDWEGFATATAAAAAAGITTLVDMPLDSDPVTTTVD
                     ALERKRQAAHGKCAVDVAFWGGVVPDNLDMLADLAASGVRGFKCFLADSGNPNFPHLT
                     AEEFRRAAARVAAMDAVLLVHAESHHVIEASPPPRGRSYASFLSSRPDASEEDAVTLV
                     IDTARHTGARVHIVHVSSARVLPMLAEAKRSGVRITGETCPHYLTFAAENILDGATQF
                     AACPPIRDADNRDLLWQGIADGTLDMIVSDHSPCAPSMKADGDFGRVFGGISSLEVGP
                     RAVWTRATKRGFGLAQLCRWMAETPAALAGLDDRGRIAVGQRADFCVFDPDVEQTVGT
                     LRHRHAVTPYDGAALRGSVAQTWVAGRLVYDRVGQPA"
     gene            complement(15649..16455)
                     /locus_tag="VFC01_23965"
     CDS             complement(15649..16455)
                     /locus_tag="VFC01_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HZN82706.1"
                     /translation="MGKVHRIILLTLGWEELPKAVSVYGAPPEERMREPVPGVLLQTD
                     GGWVLLDTGFNTALIRDPALYRRFYPTVEYIPVLPGPGEPIEESLHDIGVDIDDIHTV
                     AVSHLHHDHAGGLKLFAGKVPVHAQRRELEYGLSNHPEPEHNAIVRIDFDDPRIEWKL
                     ADGEAEIAPGITAVPTYGHTPGHQSFVVELDESVGGDGFVFAFDAADLTENIEHELAI
                     GGYIGVEPEETIEPIRKLKRLAKEKGYQLIPGHDPHVWPELTRHFHDRFA"
     gene            complement(16460..18040)
                     /locus_tag="VFC01_23970"
     CDS             complement(16460..18040)
                     /locus_tag="VFC01_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN82707.1"
                     /translation="MSKRLLAIAAAGAVAMLTLSACGGSDSGSSTPNAAPTDKVLHLS
                     FLQDPGQPPDPDVYYAGQGLLLTTNLYEGLLQFKGGQGKAEFEPLLATEWTASPDNKV
                     FTLKLREGVKFHDGTPFKSDAVKASFDRRLAVNGGPAYMVKDIESITTQGDYGVTITL
                     KSPNSEFLDYLASPYGPRMMSPTGLQKNAGSDSAQNYLTTHDLGTGPYTLTDAQVGSH
                     YAMASFPEYWGAKPYFEKVEMPVITDTSAQQLQFNNGQLAAILHDLPSSAVEQYLNNS
                     KYSHYSLPTMMASFLYVNPNKGIMADQATRTAVMDAIDVDQLVKQTYFGRGKVGEQMY
                     PPYVLAADFGKQTLKHDPAALTKIAAGLPADQKTITIGYDTSNPDMQLVSNLVQTQLA
                     AAGITAKVQGYPTSEIYGWIGTDMQAAPEIFTNMVWPDAPSPYTWGHISWDKDGGINY
                     LGCSSPRVTDALAQGLPNGAPQPYSDAGAAAEETGCWLNVADVDDFVVAQPWLKGVEQ
                     AHAVSNPNSLRIAALSVG"
     gene            complement(18037..18567)
                     /locus_tag="VFC01_23975"
     CDS             complement(18037..18567)
                     /locus_tag="VFC01_23975"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HZN82708.1"
                     /translation="MPGHAERVERAEKLVSAARAAGVPVVFFQEVHRPSGVDFGRELD
                     GTEGVHCIDGQPGTDLEPSLRPLPDEFHIVKRRYSGFIGTDFEIVLRGLRASTLILIG
                     GLTDVCVHYTFADAHQRDYYVRVVTDCVGGSSQYLHDAALAAMEYLQTGAMRTTEEIL
                     TAFQALEQPVLEGAAR"
     gene            complement(18667..19671)
                     /locus_tag="VFC01_23980"
     CDS             complement(18667..19671)
                     /locus_tag="VFC01_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN82709.1"
                     /translation="MRNFILHRLGAMVLILIALIAVMFVLQHISPMDPVKAQMGANAS
                     ADAVAARRHTLGLDQPMVNQFWNYLTAAVRGDLGQSYRTRHAVSSDLGDFFPATLELA
                     LYGLGLALILAGLLAFGTTLKWPGASVLRAVLFTGASAPMFLLGILGLIVFYQKLGWV
                     PANGRISVPDPPTGPTGLLTVDGLIAGRFDVVTNALHHLILPAVVIALGPAVAIGRVL
                     RSSLLTDADSDYARTARAKGLSEGRIMVGHVLRNSVGGALAMTGLQVGLMFSGVLVVE
                     QVFGWPGIGQYIAQSIPVADFPAIAGVTLVLGMLYVAINTAVDLLQAAADPRIAVGGG
                     "
     gene            complement(19675..20541)
                     /locus_tag="VFC01_23985"
     CDS             complement(19675..20541)
                     /locus_tag="VFC01_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN82710.1"
                     /translation="MAIALAAGTVTRGRVRGLSLPTSSPMLRNWAAVSLVLIVTIVAI
                     AVPVISPHDPLVPAGMPLQAPGKGGFLLGTDAIGRDIGSRVLYGIRASWFAALVVVAV
                     GLLIGGLVGLIAGAAGGWIDSLLMRITDAFLSLPAPVLAIAVVAALGPGFIHTLIAVS
                     IVWWPFYARLVRGEVARLAARPHIEAARLAGVGPIRLAGRHLLPGAVPNALVAASLDI
                     GNLILTLAALSFLGLGQAAPAPELGADTARNLTYFLQQWWIPVMPGLAVLVLALAANL
                     AGDGLRNLMKTS"
     gene            complement(20543..22501)
                     /locus_tag="VFC01_23990"
     CDS             complement(20543..22501)
                     /locus_tag="VFC01_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN82711.1"
                     /translation="MSSAVTEVDAHSSSPAADAAQVATITDLHVTFRRNGRDVHALRG
                     VSLDIAPGEILGLVGESGSGKSVLGFTMLGLLPQARVQGSVTVTGTDMVTGDAKTLRK
                     VRRLDLGAIFQDPMTSLNPTMRIGRQVAEAAGSDEEALKLLTAVGIPEPKRRFRAYPH
                     ELSGGLRQRVMIAIAIAGNPELIIADEPTTALDVTVQAQVLRLLLRLRDEIGCSIMFI
                     THDLGVAAQISDRIAVLYAGRIAEIGPTAEVLNQPAHPYTHGLLRSRLTLQTARHRKL
                     AALAGSVPSPVTPLPGCAFAPRCVVATPACEATPPDPITVAPGRVSACIVPFEAVTTE
                     LGSRLTTTTEDIEPETESTETSEPPAVVLQDVVKAFGKLQALRGISLTVGHGESVALV
                     GESGSGKSTLLRVIAGLEKASSGTVELAGVQGPQMVFQDAGASLTPWLSVGELIAERL
                     RGNGLSRSQRQDAVAEVLQRVGLPLDIAKSRAGQLSGGQRQRVSLARATVVPPAVLLC
                     DEPTSALDVSLAASVLNLIGDLRRTLDMSVVFVTHDLSVARVVADRIAVMYLGRIVEI
                     GPADDVINNPVHPYTQALVDSIPDLGRESRVLPGEPASPLSPPSGCAFHPRCPIAVDT
                     CSDNQLDVRLEGAAGSPHQVACIERKAS"
     gene            22673..23347
                     /locus_tag="VFC01_23995"
     CDS             22673..23347
                     /locus_tag="VFC01_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN82712.1"
                     /translation="MSITRAGRPRLTTQRRPGTTARDEILDAAGELFTTLGYTGTSTR
                     TIAEAVGIRQASLYHYFKTKDDILCALLSQTVTPTLMVVESLAGTEPSLSAAEHLHAL
                     ALFDGRQLLDGRWNLGALYLQPELRGARLEPFWSDRERLRLHYLAVSKAIVADTGIDE
                     AAADLPFRLVESLVNMWTARPGPERTRLPEHIADACIRVLGMPDSVTPRLRARSHLEL
                     TRYAAD"
     gene            complement(23344..>23585)
                     /locus_tag="VFC01_24000"
     CDS             complement(23344..>23585)
                     /locus_tag="VFC01_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN82713.1"
                     /translation="GPRRAVANPLNIQLSGFPAKAVTRLYHLYAIPRFVNRWAVALAY
                     LTDVFFDRSVVSIGLASEEDAQFAASEGIPMPKAD"
BASE COUNT         3933 a         8017 c         7751 g         3884 t
ORIGIN      
        1 ctcgagcgcc tcgtgcccgc aggatctcag caaccgtgcc tggtgcgggt catccccggt
       61 gtcgaggcag cgactcacgc ctcacaggca acgggtcacg ccggctcgaa gttcggcctg
      121 ctgcccgacg acgcccgcga cgcgatcgcg cgaatcgagc gcagtgccaa gctgcgactc
      181 gacggtgtgc acacccacgt tggatcgcaa ctgctcaacg tcgaacagct cgcgcaggcc
      241 gtcgaaccga tcgcgaagct cggcaccttc gacgtctacg acctcggcgg cggcctcggg
      301 gtccgctaca cctacgacga acacccgccc agcctcgacg actacgccga cgcgatggtg
      361 acccaggcca aggcgttgct gccggaaggt gcccggatca tcgtggagcc gggccgcagc
      421 atggtcgcca acagcgcatg cacggtctac cggatcacca cgatcaagcg cggcgtcgtc
      481 acccacgtgg cggtggacgg cggaatgggc gacaacctcg aggtatcgct gaccgggcag
      541 cggttcgaag cgacgatcgt caaccgcgtt ggcggcggag aaatggtcag cgttgtcggc
      601 aggcactgcg aatccggcga ccaactcgtc gacggcgtac cgctgcagca acccgcaatc
      661 ggagacctac tcgcggtgcc ggtcaccggc gcctactgct acacgatgtc caatcagtac
      721 aacggtgccc ggcgcgtccc ggttgtcttc gcccgcaacg ggactccccg gctcgtggtg
      781 cgccgggaca cctgggacga cttgttgatc cgcgatgtcg actgacagcg tgcaagatag
      841 gcggcatgac tgagctgccc gactcgcttg tcgagttggc gcggcgctac ggcgtcgcaa
      901 ccgaattcga ggactggacc ggcaggcgca cggccgtcgc cgatagcacg ctgatcgccg
      961 tgctcgacgc cctcggggtg gacgcggcca ccgaggacga gcgcgccgcc gcactggccg
     1021 cccacgaccg cgaccactgg tcgcactcgc tgccgccgac gatcgtcggc cggtccggcg
     1081 tcgcatcccc gttctgggtg cacgtcacgc acggcgagcc ggccgggcta tggcttcggc
     1141 tggaggacgg ctccgtacgc accgggctgc gtcagctcga aaacaacaga gcaccttacg
     1201 atctcgatgg ccaattgatc ggcgaggcca ccttcgagct acccgacgac ttgccgctgg
     1261 gctatcacca actccacctg caggtcggtg catccgacgt caacacggta gtgatcgtca
     1321 cgcccgcgtc gctgggcctg cccgccaggc tgggcacgag ccgggcatgg gggctggcca
     1381 cccagctgta cagcgtgcgc tcgcaacgat cttggggcat tggcgatttg accgacctgg
     1441 ccgacctggc agtgtggtcg gccgcgcagc acggcgcggg tttcgttctg gtcaacccgt
     1501 tgcatgcggc ggcccctacc ccgccgatgg agccgtcgcc gtatctgccg acgtcgcgcc
     1561 ggttcatcaa tccgctgtat ctgcgggtcg aggcgataga cgagttcgca tacgtgcggc
     1621 agcgcggcag catccgcaag gcgcgcgtgg aagtgcaggc ccacgcgaag aaatcgaagc
     1681 ggatcgaccg cgacgccgtg tggaaggcca aacgcgctgc gttgaaaagc gtctaccgcg
     1741 tccagcggtc ggcgggccga gagctggcgt acgccgccta ctgccaccgc gagggccgga
     1801 gcctcgacga cttcgcgacc tggtgtgcgc tcgccgagga gtacggcaac gactggcacc
     1861 agtggcccga ggaactgcag catccggccg gcccggccgt ctccgagtac gccgccaaac
     1921 acgcaaagac cgtcgacttt caccgctggc tgcagtggca actcgacgac cagctgaccg
     1981 ccgcgcaggc cactgccgtc caggcgggca tggacctcgg cgtcatgcac gaccttgcgg
     2041 tcggcgtgga tcccaacggt gccgatgcgt gggcgctgca ggacgtgctc gcgctcggcg
     2101 tcaccgcggg tgcgccgccc gacgagttca accagctcgg ccaggactgg tcgcaaccgc
     2161 cgtggcggcc cgatcgcttg gtcgaccagg catacgaacc atttcgcgcg ctggtcaatg
     2221 cggtgctacg acacgcgggc ggcgttcgta tcgaccacat catcggcctg ttccggctct
     2281 ggtggatccc aaagggcgcc ccgccgaccg acggcaccta tgtgcgctac gaccacgagg
     2341 cgttgatcgg catcgtcgcg ctcgaggcgc accgggccgg tgcggtggtg gtcggcgaag
     2401 atctcggcac cgtcgagcca tgggtccgtg actacctgcg cgaacgcggc ctgtttggca
     2461 cgtcgatcct gtggttcgag gccgaccgcg acggtgatgg cggaccgctg cccgccgaga
     2521 attggcgcga gttctgcctg tcgtcggtga ccacccacga tctgccgccg acagcgggct
     2581 atctcgcggg tgagcatgtc cggctgcgcg aaaagctcgg cctattgacc cggcccgccg
     2641 ccgaggaact ggccagtgac caggcccaac aagcggcctg gctggccgag ctgcgccggg
     2701 tcgggctgct cggcgacgac ccgagcgtcg aggaggtcgt cctcgcgctg taccggtatc
     2761 tgggccggac accttcgaag ctgctggcac tgtcgctcgt cgacgcggtc ggcgaccttc
     2821 gcacgcagaa tcagccgggc accaccgacg agtacccgaa ttggcgggtg ccgctgcggg
     2881 ggcccgacgg ccgaaaactg ctgctagagg acgtattcac cgacccccgg gcggctacgc
     2941 tcgccgaggc gatgaaacat atcgtgcacc cgccggtgta acgcttcggc gcctgtctcc
     3001 ggtttcacat ttgtcaccgc tgcgatatgt tcgcaccgca ccgacttagg gagatcacgt
     3061 gaagaagatc gttgtgctca gcggtggcct cgtcgccgcc ggctcggccg ctctgatcgg
     3121 cgctggtgtg gctctctcgc agcctcagcc ggacccgagc tcgtacaacg tggtcggtga
     3181 gccgtatcag aaggcgctga tgattctgaa gagccagggc gtgaaggcct acttcggagg
     3241 ctcgttcggc agcgtgttgc cgcaggccca gtgccttgtc gaccagcaga agatcacctc
     3301 gggcggccgg atgctgctgt cgctcgactg cagccaaaac gccgccgaca ttctcgcggc
     3361 gatgggcccc tccggaggtc cgacggtcgg ctccaacggt gtgaccacgg tgaccccgac
     3421 cccggtggta ccgattcagg gcgcacccgg cccggccgcc ccgccgcctg cctaggcctc
     3481 gaccagctgt ttcaggtttt tcagcgtctc ggtcatgttg cgaccgacct gacgcgcggc
     3541 cacccgatcg gcgatcagcc cgagcagatt gcctgccgat tcgtaggcca gccggaagat
     3601 gaccttggtg tggccgtcgc cgttgtcgcg gattcggaac cgaccgcgca gcgagacgcc
     3661 tgaaacgccg atccaggcaa ggtcgtgggc cgggtcgaac tcagagacct cgacgacacc
     3721 gccgatcggc accgagcgga tcttccagtg caccgtgtag cgggacccct ttctggcggg
     3781 gccctcggtc acgtgttccc agcgttcgag gctggccatg aactccggat agcagtccgg
     3841 gtcgctgacg accttccata cggtgtcgcg gtcggcgtcg atgacgcagt gacgttggac
     3901 gcgcatcacg cacctcgctt cgtcgcgcaa gcgctcatca gccgattatc gggaatcggc
     3961 gctccgcggc cagtcggtcc aggcccgcct gcaggctttc caccaccttc ttgtgcacga
     4021 cctcgtcgtc gccgtccacg tcaatgggat cgagtacctc gacgacgatc ttggcgggca
     4081 gcgggatgtg tcccaacagg tcgccgatgt tgaggcccca cggcagggcc agcgagatcg
     4141 gcacgctctt gagccgggcc agcttgtcca ccatcaacag cttggccagc cattggccgc
     4201 ggtcgaggaa cagtgcggtc tcctggccgc cgacgctggc gatcggcacg atcggcacac
     4261 cggcctcgcg agccagccgg acatagccct tgcggccgcc gaaatcgacc ttgtgccgct
     4321 cccacgacgg ccggaacacc tcgtagtcgc cgccgggata caccagcagc gcggcaccgg
     4381 attccagtgc catgcgggca ttctcgtgat tggcggcgac ggtgccgaac ttgcgcagcc
     4441 aacccagcgg cggcgcggag acgacgaggt tgtgggcgag ttgatagaac ggccgctcca
     4501 cgccgaaata ggagcagaag gccagcgtga acacgaacgt gtccggtggc acgttgccgc
     4561 cactgtgatt gccgaccagc agcaccgggc cctcggcggg aatgcggtcc aggccacgca
     4621 cgtcggcccg gaaatacagc gacgccagca accaggttcc gggcagctgc tcgcggatgt
     4681 agtccgggtc gcgctgatcg aggtcggcct tcgggacccg cgacgagacc tgatgcctag
     4741 cccattcgac aacgtcgcct aaccccgcca tgtcagtcgt atatacccgg ccggcccgca
     4801 cctcaagccg atacccgcgt tcccacctac ttcgctagac ttccgagcat gctgcgaccg
     4861 caccgtttcg acaccgaagt cgatccgggc ccggtacaga tccaggcccg caaggttcat
     4921 ttcaatgtct cgggcatccc gctgcattgg attccgggac atccggttgc ctcgaacgtc
     4981 atcagcctgc tcaacatcgt gctacctgcc gccgagcgct ggttcgtgct gaccctcaac
     5041 gaggcgttgc cattggtgaa ggatccgcat ctggccgagg acatgcgtgg cttcatcggc
     5101 caggaggcca tgcacgccga cgtgcacgag caggtgctgc acgagtacat ggtgatcaat
     5161 ggcatcgacc ccaagccgat cctcgatcag gtcgagtatg ccttcgaaaa ggtattggcg
     5221 ccaacaacat ccaccgatcc caagcgccgg ctcaaccacc tgtgcgatcg gctgtggctg
     5281 atcgcggccg tcgagcacta caccgccgtc atgggtgatt tcgcgctgaa ctgcgcatgg
     5341 gacgaccacg atgcctcacc gacgatggtc gacctgttcc ggtggcacgg cagcgaggaa
     5401 gtcgagcacc gcatggtggc ccacgacgtc gccgtgtact tccacgacag ttacatcgac
     5461 cggatccgcg ccatgtccat ggcagtcgtg atgatcttcg tgatgttcca gcgcgcggcc
     5521 tggtacatcg tcaagaccga ccccgacacc gacatcggct ggtggaaatt caatcggatg
     5581 cgaatgcggg actccaagct tgggctgctt cccaagtacc gcaagctgtt tggctccaac
     5641 accgcgatgt atttcaggcc ggggtactcc cccgaggaga tggggtcgac cgcgcaggcc
     5701 gtcgcctacc tcgccagttc ccccgcggcc cgcgccgcgc acctttgatg gggctcctgg
     5761 agcggtaccg gcaggcgcca cccagtgcct tcgggcggct gcgccacgac gtcgtgatgg
     5821 gcctggcaga cgtcaccctc aacggtctgt gggcgatctc gggcgcgatc cggcgcatgt
     5881 cggcaccgcc cgacaccgac cgcaccatcg cgctgacggt gaccgaccga aaggtggtcg
     5941 cccatgacca ggacgtcgtc acgctgacat tggtcggcga acagttgccg cgctggcatc
     6001 caggtgcgca catcgacatt catctgccca gcgggctggt gcggcagtac tcgctgtgcg
     6061 gagatccggc gacacccgac acctaccgca tcgcggtgcg acgtatcccg gatggcggcg
     6121 gcggctcgat agagatgcac gacttgcggg tcggcgcgac ggtgacgacg catggcccgc
     6181 gcaatgcgtt cccgctcacg gtgcccggct acggatcacc cgcgcggcgg ttccgcttca
     6241 tcgccggcgg catcggcatc accccgatcc tgccgatgct ggggctggcg cagcggctcg
     6301 ccgtcgactg gtcgatggtc tacgccggtc gcagccgcga aagcctgcca ttcctagacg
     6361 aagtgagccg gtttggcgac cgcatcgagg tccgcaccga tgacgtcagc ggactgccca
     6421 cagccgcgga gttgctcggt ggctgcccgg acggtactgc cgtgtacgca tgcgggcctg
     6481 cgcccatgct gacggcgatc cggtccgcac tcgccggccg ggacgacgtc gaattgcact
     6541 tcgagcggtt cgccgcaccg ccggtcgtcg acggcaagga cttctcggtg tcggtcgccg
     6601 cgaccggcca gacggtgcgg gtcggcgccg acgagacgct gctgtcggca ctgaatcggg
     6661 ccggcgtgca cgcgccgtac tcctgccagc agggtttctg cggcacatgc cgcaccagag
     6721 tggtcgacgg cgccgtcgac catcgggaca ccttgctcac cgatcctgag cgcgacagcg
     6781 gcatgatgtt gatctgcata tcgcgggcgg ccgacggctc gcatctgacg ctcgatctat
     6841 agccgcctct gtactattcg cccatgccgt tggccgatgg tgcgacgttc gccggctaca
     6901 ccatcgtgcg gatgctcggc tcgggcggca tgggtgaggt ctatctggcc cagcatccgc
     6961 ggctgcctcg ccgcgacgcg ctcaaggtgc tgcccgcgtc ggtgtcggcg gacagcgagt
     7021 atcggcagcg gttcaaccgc gaggccgaca tcgcggccac gctctggcat ccgcatatcg
     7081 tcggcgtgca cgaccgcggc gacttcgagg gccagctgtg gatctcgatg gattacgtgg
     7141 acggcaccga cgccagccgg ctgctgcgcg agcgctatcc cagcgggctg cccaaagccg
     7201 aggtcgccaa catcgtgacc gctgtcgccg aggcgctgga ttacgcacat gagcgcggcc
     7261 tgctgcaccg cgacgtcaag cccgccaaca tcctgctggc ctacccggat tcgggagatc
     7321 agcgaatcct gttggccgac ttcggcatcg cgcgctgggc tgacgacatc agcggattga
     7381 ccgaaaccaa catgaccgtc ggtaccgtgt cgtacgcggc gcccgaacag ctgatgggtc
     7441 agcagttgga cggacgggcc gaccaatacg cgttggcggc caccgctttt catctgctga
     7501 ccggcgctcc gccatttcag agctccaacc cggcggtggt catcagccag catttgtcgg
     7561 catctccgcc ggcggtcggc gcgcagcgac ccgaactgtc ggcgctcgac ccggtccttg
     7621 ccaaggcact ggccaaggat ccgaaggacc gtttcgagcg gtgcgccgac ttcgcccgcg
     7681 cttttgccca ccgcctcgat gcggcgccga ccggtgatgt cgacaccggc ctcgcctccg
     7741 ccgcaaggcc gcagaaacgc tcgctgctgc gggccggtgt catcgtgccc gcggttctcg
     7801 ccctcctact gatcgcggca attacggtgg cgatcatgga gtttcgccgc gcggaccgtc
     7861 ctgagacgcc tactgcaacg gcaacatcgc cgacggccac caccccgccc gtcactagcc
     7921 cgacgttcac accgctgcct ccgccacccg agccggtgcc accgcccgcc gcagtgatcg
     7981 gcgcgaactg ctcacccgag ggcagcacag ccacgacacc cgagggagcc accgcatact
     8041 gctcgacgct gcaaagcacc ggcgccacga tctggtcgct caatcccggc gatgtggcag
     8101 aaccgaccgt caccgccgca ccgaccgatg agccgctgcc cgtcgcagag gagaacccgg
     8161 tgcgtgtctg catgcagcag accggcagaa cccggctggc ctgccgagag atgatccgac
     8221 gcagcaacct tgggctgcca tgaaattcgc cgtcatagcg cccgtcgcgg ccggggtcac
     8281 cgccgacccc gaatggatgt ccggcttcgc cagacacgtc gaggcctgtg gattcgagtc
     8341 gatcgtcgcc gtcgaacaca cggtgctgat gaccgagtac cgcagcgtct acccatatgc
     8401 ggaatcggga cgggtggagt tgcgcgccga ctgcccggtg cccgatcccc tcgatctgct
     8461 gacattcctt gcggcgcata ccaaccggct cggcctggcc accggtgtgc tggtgctgcc
     8521 caaccaccat ccggtggtgc tggccaagcg cgtggctacc gtggatgcgc tgtcaggtgg
     8581 ccggattcga ctctgcgtgg gaatgggttg gctcaaggag gagatcgagg cgtgcggcgc
     8641 ggagttcgcc agccgagggc gccgcgccga cgaacagatc gaggtaatgc ggctgctgtg
     8701 ggaagaccgg cccggcggcg cgagctactc cggccaattc gtcgaattcg accacgccac
     8761 ttgctatccc aagccgacag gtcgggtgcc catccacatc ggcgggcaca gccgcgcggc
     8821 cgcccgccgc gctggccggc tgggtgacgg ctttcagccg ctcggcgtcg gaggtgccag
     8881 gctgagcgaa ctcatcggga tcatgcgcga cgaggcgaac aaggccggcc gcgaccccga
     8941 tgcgctcgag ctgtcgctcg gccacttggt gaccaaggtc gacggtgagc gagccgacaa
     9001 actcgcgggc cttggcgccg acagaatcgt gctggccatg ccaccgaccg ctgacctggg
     9061 cgaggcctgt gacgtgctgt cggcctgcgc gcgacggttg gcgctgatcc catgaacctg
     9121 aatgcctatg accggctcgc cctggccgat ctcgtgcacc tgtacgcgtc ggcggtcgac
     9181 gaccggcggt tcgacgatgt agtcgaactg ttcaccgaga ctgcggaatt acgactgccc
     9241 gacccgcccc gatcgctcga gccggtgcgc catcatcagg ggcgcggcgg ggtgcggcag
     9301 gcgatggccg cgctcgcagc ggtgacccgc accgagcatg cgatcgtcgg ggaggtctac
     9361 gccccgggcg atgattcgga ctacgcgctt ggccgaatta cgtgcatcgc tcaccactgg
     9421 accgccgacg cggatcaaat caccgatctg gtctggcatc tgcgctacga cgatgagtac
     9481 ctgcgcacgc gcgcagggtg gcgtatccac ggccgcgcgc tgaccatcaa cgctatcgag
     9541 acccgcccgg ttcgtcgcct tcgagggcag cggtgagacg gtcaagcagc ggacgctgac
     9601 ccgtcaagag cttctcgttg gcctcggtca cggaaaacca accgacgcgg tcgatttcgg
     9661 ggtattcgcg gatgttgcct gaccccttgg gccactcgag ttcgaaggtg ttgctgaagg
     9721 tgccttcgag atcgaggtcg ccgcgcacgg cgaacgccgt gatgatcttg ccgctcggct
     9781 gcttcaaggg cacgaagtcg atgcgcggcc cctccggcgg cgctttgccc agttcctctt
     9841 cgaattcgcg ctgcgcgacg gtccatgggt cttcgccctc gacatactcg cccttcggaa
     9901 tcgaccacgc cccttcgtct ttgcgtgccc agaacgggcc gccgggatgg ccgatcagca
     9961 cctcgacgcc gccgtcggtg acccggtaca gcagcaggcc cgcactcagc ttcggcatca
    10021 ggcggtcgtc ggttcgctga gcttgaccag cgtcttgccg atgttggcgc cggtgaacaa
    10081 cccgttgagg gcgtcgacgc aggattcgat gccctcgaag atggtctggc ggtgtacgag
    10141 tcggccgtcg gcctcccact gccgcagcga cgcgaacgcc tcgtcgaagc ggccccactc
    10201 gtcgagcgca ttgaatcctt gcatcgaagc agtgcgcgcg agcaggttga cgtagttggc
    10261 ggggccgggg tgttcgccgg tcaggtagct ggagatgacg ccgcacagca cgacccgcgc
    10321 cttgggggcg agccggccca gcaccgcgtc gaggatgggc ccgccaacgt tgtcgaagta
    10381 gacgttcact cccttggggc aatgctcttt gagcgccgcg ggcaggccgg cctccttgta
    10441 gtcgatgcac gcgtcgaacc cgaagtcctc gaccaccgcc ttgcacttgt gtgggccgcc
    10501 cgcgatacct accacccgcg ctccggcgat cttggctatc tgcccggcca ccgacccggt
    10561 agcacccgcc gcggcagaga cgaccacggt ctcccccggc ttgggcttgc cgatgtcggt
    10621 catcccgaag taggcggtgg cgccggtggg gccgtagacg gacatgatcg cgagctgatc
    10681 ggtctcgccg gggatgggcg tggtgaagat gtcgtcgcgg atgatgacgt actcctggaa
    10741 gcctgtcagc gtggtgacga cgtcgccgac ggcgtaggcg tcgcagcgtg atgccaccac
    10801 ctcgccgatt cccgccgccc ggatgacgtc gcccagctgc agcgcgggca gatagccggg
    10861 ctggccgtcc agccaggtac gggcggcggc gtcgatcccg acgtaggtgg tgcgcaccag
    10921 cgcctcgccg tcggcgggct cgggtgccgg gttggtgacc agctcggtgt cgtcgggccc
    10981 gaccagcccg gtcgggcggc ggcgcagcag gatctggcgg ttcgtcagct caggcacatg
    11041 ggcgaaccta ccgaagacta ggcccgcttc tgcagtgctt gctgtccaaa ctgtccggtt
    11101 tccagcgagc cgtcgggtag caccagctcg ccggacttca cccggttgcg aagataggcg
    11161 aacgtctgtg cggtctgagc gaacaggtgc tcgcccgcgc gcaacgccgg ccgctgcttg
    11221 ccgtcgcgag aggcgaactg cggcaacggt ttcacctcgg tgtgatagtc gacattgaag
    11281 aacagcggga acgaataccg ttcctctttc accttccgca cccggtggct ggtcgccacg
    11341 taggcgccgt tggtccacag ttccagcatg tcgccgatgt tgacgacgaa cgtccccggc
    11401 acgggcggca cgtcgatcca ctcgcccgcg ccgttgagca cctcgaggcc cggcgccgtg
    11461 ggcttgagca gggtgaagca ttcgtagtcg gtgtgcgcac cgataccctc gccgtccacg
    11521 gcgccggggt tgtatggata gtggatgagc cgcagctggc ttggcgtctt ggtcgcgtgc
    11581 ttggagaacg tgtcgggatc ctcccccaac gccaccgcga acgcccgcaa caaccgctgc
    11641 ccgacgtcga gtaccgcctc gtagtacgcg gtcaccgcat cggcgaaacc aggtaggtcc
    11701 ggccaggtgt tcgggccgag catcggattg ccggcaaggt agtccggatc gtcggccggt
    11761 aggtccagcg cggtgtcgaa cgcctccttc atttccggca tgccggtcgc gaggccctcc
    11821 tcaccggagg ggacgtagcc ccgatggcat gcggacagcc cgatgtagta gcgcatcttc
    11881 tcgtcgagcg gcagcgcgaa gaactccttc gtcgccgcca acatgcggtc aaacaacaag
    11941 tcgtcgaccc cggcaccacc tacatagaag aacccgacct cgctggccgc cttgccgagc
    12001 tcagcggcta ctcgctcgcg ctcctgccga tccggtgatc ccagcccact gatgtccacg
    12061 atcggcaccg aagtgaatga cgttgtgcca ctcatagatc cacatctcct tcgctatcct
    12121 cgatacgcca tcggacacca ttggccacca ggtcactgcc attcacgcgc gggttgagcg
    12181 ttgtccattg ggggccgccg atcgtgtgca gcaccacacc gtttccgtct gcccagccga
    12241 agtgcgcacc ggcgcgcagc aggatcgcgt tgtgcaggaa cagcgcccag caccgaacgc
    12301 ccggatcgtc gtcgcggacc caatgctcga cgtagtcctc gtgcacgccg acttcgatca
    12361 acacgccagc atcccagcgc atccgacccg cgtcgggaaa cgggcccggc ggctcgatat
    12421 cgatgagccg ctgccactcg aagacgtcac cgtgctggcc gagccgaccg gcgaagccgc
    12481 gggtgtccac gtacgcggag atcccctgca gccacgctac atccgtgctc acgtcccgcg
    12541 atccgtcgcc ggcgatcagc aaggtgcgcc gccacaagcc gctgcattcg gtcatcaaca
    12601 cggtcatgcc gacgcctgcc acatggattc cagcagggcg atcccgtctg cggttggcct
    12661 gccgaacgcc cggacccggg caatcccacc gtcggggtag gcctgcaccc gcagcgcggt
    12721 gatatccgtg gcggccacga tgaacacctg ccgggcgtcg ggtgcgatgc gggtgcgagt
    12781 cagcacttcc acgtcgaatg gcaccagcgc ccagccgtgt tcgccgcagg gcaccccccg
    12841 gctgcccagc accgccacct cgcgtgacgc gttgaacacg aagcggctgg tgtcgacctc
    12901 gatgcgctgg agatcggcgg gcaccgcgaa cgagatcatc accgcgtcgt gggtgtcggg
    12961 accgatgtcg cgacgccgct tggtctccca tccgtcgccc atggtgcgcg gccgatccgg
    13021 agcaatgagc acgcacgcat cggaatagaa gctgtcagaa gaccattcga cgcgaccacc
    13081 ctgttcgacg cccgacacct cgacggtgat gcccgcccac gtcgcggggt cggggatgac
    13141 ctcgccgtaa acccgcagtc gcgccactcc cccgtcggat tccaggttta ggcgcacatg
    13201 cgtgtagcgc cgtgggtcgt cgacggcaaa gacgttgtgg gagtcgggct tcagcgcagt
    13261 ggtctcgacg accgtcgtcc actgcgccgc agcggcggtg gcgtcgtcga cgacgtcgag
    13321 cacgcacgca tcgatgcggc acgcggtcgg gtgattgcct gcgaagaatc gggtgtcgac
    13381 gtcgatcgcg cgcagccttc cgccggctcc gagtcgcacg atcgcccagt cgccaccgcc
    13441 ccctacgtac ctgcgggtct cccaaccgtc gacgacctca ccgcgcagat cgtaggtgcc
    13501 tggaacgaac gccggctcgc tcggctcgac cagccgttcc ttgaacccga acgattcgtc
    13561 actggcggcg acgacgctgc cgccgagggc ccgcgacgcg agatcgactt tcaccggtcc
    13621 aaccccagtg ccgtcgacaa tggccaatgg ctgcgcgggt tgtccgggcc cgcggcatac
    13681 acaccggcct tgctcgtgct cagcccgagc ccgaccaatg cctgggcgag tgcgaccgcg
    13741 gcggccaccc cgtcgatggc gggcacaccc agcttggccg tgatcaccgc ggtgacaccg
    13801 gccatccccg cacagccgag gcagatcacg tcggcgccgt cctccgcaat cgccctggcc
    13861 gcctcgtcga cgatcgcctg cacggcgcct gcggggtcgg cgtccacttc tgcggtaccg
    13921 agcccgcagg cacgcaccga cgcgcagtgc gcgtcgaggc ctgcgagcat cagccggtcc
    13981 tcgataggcg gtatcgaacg cgccaacgtg gtcactacgc agaaccgccg cccgatcaga
    14041 tgcgcgacgt gcgccgcggc ctccgcgatg tccaacaccg gtacgggcag catctcctgc
    14101 agcgcatcct tgccatgctc accgaacccg gccagaatca ctgcgtcgta atcgaattcg
    14161 cccaccgcga gcatggtggt caccacgtcc atcacgccca ccgcagacag gtagctttcg
    14221 gccgccgaat cgatcgccgt cgcgccgaat cgtggattgc gcgcgacgat ctctgtcccc
    14281 tcggcggcga cagcggctgc ggccgcggcg atctcggcgg tcatcgactc cgatgtattc
    14341 ggattcagca ccaggattct catgccggct gacccacccg gtcgtagacg aggcggcccg
    14401 ccacccacgt ctgcgccacc gacccgcgca gcgccgcgcc gtcgtagggc gttaccgcgt
    14461 gccggtgccg cagcgtgccc accgtctgct cgacatcggg atcgaatacg cagaaatccg
    14521 cgcgctgccc gaccgcgatc cggccgcggt catccagccc tgcgagtgcg gcgggcgttt
    14581 ccgccatcca ccggcacagc tgcgcgagcc cgaatccgcg cttggtggcg cgcgtccata
    14641 cggcgcgcgg cccgacctcg agcgaactga ttccgccgaa cacccggccg aagtcgccgt
    14701 cggccttcat cgatggcgcg cagggcgagt ggtcggacac gatcatgtcc agcgtgccgt
    14761 cggcaatccc ttgccacagc aagtcgcggt tgtcagcgtc gcgaatcggt ggacaggccg
    14821 cgaactgcgt tgcgccatcg aggatgttct cggccgcgaa ggtcaggtaa tgcgggcagg
    14881 tctcgccggt gatgcgcacg ccggagcgct tggcctcggc cagcatcggc aacacgcggg
    14941 cgctcgacac atgcacgatg tgcacccggg caccggtgtg cctggcggtg tcgatcacca
    15001 gcgtgacggc gtcttcctcc gacgcgtccg gccgcgacga cagaaacgac gcatagctgc
    15061 gcccacgcgg gggcgggctg gcctcgatga cgtggtggct ttccgcgtgc accaacagca
    15121 ctgcgtccat cgcagcaaca cgcgcggcgg cgcgccggaa ctcctcggcg gtcaggtgcg
    15181 gaaagttcgg gttgccggag tcggcgagga agcacttgaa gccccgcaca cccgaggcgg
    15241 cgaggtcggc cagcatgtcg aggttgtcgg ggaccacgcc gccccaaaac gcgacatcga
    15301 ccgcgcactt gccatgcgcc gcttgacgtt tgcgctccag cgcatcgacg gtggtggtca
    15361 ctgggtcgct gtccagcggc atgtcgacga gagtggtgat gccggccgcc gccgcggccg
    15421 ccgtcgcggt ggcgaagcct tcccagtcgg tgcccggttc gttgatgtgc acatgggtgt
    15481 cgacgaagcc cggcaggagc accgtggacg gcggcagccg aatcgactcc tgaccgctga
    15541 agtctccgat caaaccgatc ccgcggatca cgccgtcggc caccgcgacg gcggccgcgc
    15601 ggacctcgcc gtcgatcacc gccttctcgg tgcggatgac cagatccact aggcgaatct
    15661 gtcgtggaag tgtcgggtca gctccggcca tacgtgtgga tcgtggccgg gaatcagttg
    15721 gtagcctttc tctttggcca gcctcttgag cttgcggatc ggttctatcg tttcttctgg
    15781 ctcgacgccg atatacccgc cgatcgccag ctcgtgctcg atgttctcgg tcaggtcggc
    15841 ggcgtcgaac gcgaacacga agccatcgcc gccgaccgac tcgtcgagct cgacaacgaa
    15901 actctggtga cccggggtgt gcccgtaggt ggggaccgcg gtgatgcccg gcgcgatttc
    15961 ggcttccccg tccgcgagct tccactcgat gcgcgggtcg tcgaaatcga tccgcacaat
    16021 cgcgttgtgc tcgggctcag gatgattcga caacccatat tcgagttcgc gtcgctgtgc
    16081 atgcaccggc accttgccgg cgaacagctt cagcccgccg gcatgatcgt ggtgcaggtg
    16141 gctgaccgcg acggtgtgga tgtcgtcgat gtcgacgccg atgtcgtgca gcgactcctc
    16201 gatcggctca ccgggtccag gaagcaccgg gatgtactcc accgtcgggt agaaccggcg
    16261 atacagcgcg ggatctcgga tcagagcggt gttgaagccg gtgtcgagga gcacccagcc
    16321 gccgtcggtc tgcagcagca ccccgggcac cggctcccgc atccgctcct cgggcggagc
    16381 gccgtagacc gatacggcct tcggcaactc ctcccagccg agcgtcagca gaatgatccg
    16441 atgtactttg cccatcggcc taccctacgg acagcgcagc gatccgcagt gagttcgggt
    16501 tcgacaccgc gtgtgcctgc tcgactccct tcagccacgg ctgggcgacc acgaagtcgt
    16561 cgacgtcggc gacgttcaac cagcacccgg tctcttcggc tgctgcgccc gcgtccgaat
    16621 acggttgtgg cgcaccgttc ggcaatccct gcgccagcgc atcggtcacc cgcggcgacg
    16681 agcaacccag gtagttgatc ccgccgtcct tgtcccacga gatgtgcccc caggtgtagg
    16741 gcgacggcgc gtccggccac accatattgg tgaagatctc aggcgcggcc tgcatgtcgg
    16801 tgccgatcca gccgtagatc tccgaggtgg gatacccctg cacctttgcg gtgattcccg
    16861 ctgccgcgag ctgagtctgc accaggttgc tcaccagctg catgtcggga ttgctcgtgt
    16921 cgtacccgat ggtgatggtc ttctggtcgg caggcaggcc tgcagcgatc ttcgtcaacg
    16981 ccgccgggtc atgcttgagc gtctgcttcc cgaagtccgc ggccagcaca tacggcggat
    17041 acatctgttc gcccaccttg ccgcggccga aatacgtctg cttgaccaac tgatcgacgt
    17101 cgatggcatc catcaccgcc gtccgggtcg cctggtcggc catgatgccc ttgttcgggt
    17161 tgacgtagag gaagctcgcc atcatcgtcg gcagcgaata atgcgagtat ttcgaattgt
    17221 tcagatactg ctcgaccgcc gacgacggca ggtcatgcag gatcgcggcg agttggccgt
    17281 tgttgaactg caattgctgc gcggaggtgt cggtgatgac aggcatctcg accttctcga
    17341 aatacggctt ggcgccccaa tattcgggga aggatgccat cgcgtagtgc gagccgacct
    17401 gtgcatcggt cagggtgtac gggccggtac cgaggtcgtg ggtggtgagg tagttctgcg
    17461 cagagtccga tccggcgttc ttctgcagcc cggtcgggct catcatgcgc ggcccgtagg
    17521 gcgaagcgag gtagtcgagg aactccgagt tgggtgactt cagcgtgatc gtcacgccgt
    17581 aatccccttg tgtggtgatc gattcgatgt ccttgaccat gtacgcgggc ccgccgttga
    17641 cggccagcct gcggtcgaac gacgccttga ccgcgtcgga cttgaacggc gtgccgtcgt
    17701 ggaatttcac gccctcgcga agcttgagcg tgaagacctt gttgtcgggc gacgcggtcc
    17761 actccgtggc cagcagcggc tcgaactccg ccttgccttg gccgcctttg aactgcaaca
    17821 ggccctcgta gaggttcgtc gtcagcagca agccctgccc ggcgtaatac acgtcggggt
    17881 ccggcggctg accgggatcc tgcaggaacg acagatgcag caccttgtcg gtgggcgccg
    17941 cgttgggtgt gctcgatccg gaatccgaac cgccgcaggc cgaaagggtc agcatcgcga
    18001 cggctcccgc ggcggcaatg gctagaagtc gtttactcat cgtgctgctc cttctaaaac
    18061 gggttgttct agtgcttgga aggcggtgag gatctcctcg gtggtgcgca tcgcgccggt
    18121 ctgcaggtac tccatggcgg ccagtgccgc atcgtggagg tattgcgatg acccgccgac
    18181 gcaatcggtg accacgcgca cgtagtagtc gcgctggtgt gcgtcggcga acgtgtagtg
    18241 cacgcagaca tcggtcaaac cgccgatgag aatcagcgtt gacgcccgca gcccgcgcag
    18301 cacgatctcg aaatccgttc cgatgaaacc ggagtagcgc cgcttgacga tgtgaaattc
    18361 atcgggcagc ggccgcagcg acggctcgag gtcggtgccg ggctgtccgt cgatgcagtg
    18421 cactccctcg gtgccatcca attcacggcc gaagtcgacg ccgctgggcc ggtgcacctc
    18481 ctggaagaac accaccggca caccggcggc gcgcgccgcg gaaacgagtt tctcggcccg
    18541 ctcgacacgt tcggcatgcc cgggcatgtg gggaattcac acatctgcca ccggcattgc
    18601 gccgccttgc tggatgtcga cgacaaccag cactgggttg cccacgatga gtggctgtgt
    18661 tggcacctaa cctcctccta ctgctatgcg tggatcggcc gcggcctgca agaggtcgac
    18721 cgcagtgttg atggcgacgt agagcatgcc gagcaccagg gtgactcccg cgattgcggg
    18781 aaagtcggcg accggaatgc tttgggcgat gtactgaccg atgccgggcc agccgaacac
    18841 ctgctcgacg accagcacac cggagaacat caagcccacc tgaagccctg tcatcgccaa
    18901 tgcaccgccg acggagttgc gcagcacgtg gcctaccatg atgcggccct ccgaaagacc
    18961 tttggcccga gcggttctcg cgtaatcgct gtcggcgtcg gtcagcaggc tggagcgcag
    19021 cacgcgcccg atcgccacag ccgggccgag ggcgatgacc accgccggaa ggatgaggtg
    19081 gtgcagcgcg ttcgtgacga catcgaaccg cccggcgatc agcccgtcga ccgtcagcag
    19141 cccggtcggt cctgtcggcg gatccggcac gctgatccgg ccgttggcgg gcacccaccc
    19201 caatttctgg tagaagacga tcagcccgag gatgcctagc aggaacatcg gcgcggatgc
    19261 gccggtgaac agcaccgcgc gaagcacact cgcgcccggc cacttcaatg tcgtaccgaa
    19321 ggccaacagc ccggccaaga tcagcgcgag accgagcccg tacaacgcca attccagtgt
    19381 tgccgggaag aagtcgccga ggtcgctgct cacggcgtgc cgggtgcggt acgactggcc
    19441 gaggtcgccg cgaaccgccg cagtcagata gttccagaat tggttgacca tcggctgatc
    19501 gaggccaagc gtgtgtctgc gggccgcgac ggcgtccgcg gaggcgttcg cgcccatttg
    19561 cgccttgacc ggatccatgg gcgaaatgtg ctgcagcacg aacatcaccg cgatgagcgc
    19621 gatgaggatg agtaccatcg cgccgagccg atgcaggatg aagttgcgca tgagctagct
    19681 cgtcttcatc agattgcgta gaccgtcgcc cgcgaggttg gcggcaaggg caagaacgag
    19741 gacggcgaga cccggcatca ccgggatcca ccactgctgg aggaagtacg tcaggttgcg
    19801 cgcggtatcg gcgccgagct ccggcgcagg cgcggcctgg ccgaggccca ggaacgacag
    19861 cgccgccagc gtcaggatca agttgccgat gtcgaggctc gcggccacca aggcgttcgg
    19921 caccgcgccg ggcaatagat gcctgccggc caaccgaatc gggccgaccc cggccagccg
    19981 ggcggcctcg atgtgcgggc gggccgcaag gcgggccact tcaccgcgga cgagtcgtgc
    20041 gtagaacggc caccacacga tcgacaccgc gatcagcgtg tggatgaagc caggaccgag
    20101 cgcggcgact acggcgatgg ccagaaccgg cgcgggcagc gacaggaatg catcggtgat
    20161 ccgcatcagt aggctgtcga tccacccgcc cgcggcgccg gcgatcaaac cgacgagtcc
    20221 gccgatgagc agcccgaccg cgacaacgac cagcgcggcg aaccagctcg cccggatgcc
    20281 gtagagcacg cggctgccga tgtcgcggcc gatcgcatcc gtgccgagaa ggaaaccccc
    20341 cttgccaggg gcctgcagtg gcatgcccgc gggcaccagc ggatcgtgag gtgagatgac
    20401 gggcaccgcg atcgccacga tcgtcacgat gagaaccaga ctcactgcgg cccagttcct
    20461 cagcatcggc gacgaggtcg gaagcgaaag cccacgtacc cgccctcggg tcactgtgcc
    20521 tgcggcaagt gcgatggcca tttcagctcg ccttccgctc gatacaggca acttggtgtg
    20581 gactgccggc tgcaccctcg agccgaacat cgagctggtt gtcggagcag gtatcgacgg
    20641 cgatcgggca gcgcgggtgg aatgcgcacc cgctcggcgg ggacagtggg ctggcgggtt
    20701 cgcccggcag cacccgtgat tcccggccga gatctggaat cgagtcgacc aacgcctggg
    20761 tgtaggggtg gaccgggttg ttgatgacgt cgtccgccgg tccgatctcg acgatgcggc
    20821 ccaaatacat gacggcgatc cggtcggcga ccacccgcgc gaccgaaagg tcgtgcgtca
    20881 caaacaccac ggacatgtcc agcgttcggc ggaggtcgcc gatcaggttc aggaccgagg
    20941 cggccaacga gacgtcgagt gcgctggtcg gctcgtcgca cagcaggacc gccggtggca
    21001 ccaccgtcgc ccgggccagc gacacccgct gacgctgacc gccggagagc tggcccgccc
    21061 gcgacttcgc gatgtccagc ggcaggccca cgcgttgcaa cacctcggcc actgcgtcct
    21121 gtctttggct gcgggacaag ccatttccgc gcagccgctc ggcgatcagc tcgccgaccg
    21181 acagccacgg tgtcaacgat gcgcccgcgt cctggaacac catctgcggt ccctggacgc
    21241 ctgccagctc gacggttccc gagctcgctt tctccaggcc ggcgatgact cgcagcagcg
    21301 tggacttgcc cgatccgctc tcgccgacca gtgcaaccga ttcgccgtgg ccgacggtca
    21361 gtgaaatgcc gcgcagcgct tgaagtttgc cgaaggcctt gaccacatcc tgcagcacca
    21421 ccgcgggcgg ttcggacgtt tcggtggact ctgtttccgg ctcgatgtcc tcggtcgtgg
    21481 tggtgagccg cgagccgagc tcggtggtga ccgcctcgaa cggcacgatg caggcgctga
    21541 ctcggccggg ggcgaccgtg atcgggtcgg gcggggtcgc ctcacacgcc ggcgtggcca
    21601 ccacgcagcg aggggcgaat gcgcagccag gaagcggcgt caccgggctg ggcaccgacc
    21661 cggcgagcgc ggcgagctta cggtgtctgg cggtttgcag cgtcagccga gagcgcagca
    21721 agccatgggt gtaggggtgg gcgggctgat ttagaacctc tgccgtgggg ccgatctcgg
    21781 cgatgcggcc ggcatagagc accgcgatgc ggtccgagat ctgcgcggcg acgcccaggt
    21841 cgtgggtgat gaacatgatg ctgcagccga tttcatcgcg cagccgcagc agcagccgca
    21901 atacctgcgc ttgcaccgtg acgtcgaggg ccgtggtggg ttcgtcggcg atgatcagct
    21961 ccgggttacc ggcgatcgcg atggcgatca tcacgcgctg ccggagccca ccggaaagct
    22021 cgtgcgggta agcccggaac cggcgcttgg gttccgggat gccgaccgcg gtgagcagct
    22081 tgagcgcctc ctcgtcgctg cccgccgcct cggcgacctg cctaccgatc cgcatggtcg
    22141 gattcagcga cgtcatcggg tcttggaaga tggcgccgag gtcgaggcgg cggactttgc
    22201 gcagggtttt cgcgtcgccg gtcaccatgt cggtgcccgt caccgtgacc gagccctgca
    22261 cccgcgcctg cggcagcagc ccgagcatgg tgaaccccag gacgctcttg cccgagccgg
    22321 attcgccgac caggccgagg atctcgccgg gtgcgatgtc gagggacacc ccgcgaagcg
    22381 catgcacgtc gcggccattg cggcggaacg tgacgtgcag atcggtgatc gtcgcgacct
    22441 gcgcagcgtc ggccgccggg ctcgacgagt gtgcgtcgac ctcggttact gcgctactca
    22501 tccaccgctc ctaaaccctc ccggcctgtc acttgacagt tttcagggcg cgccgtggcg
    22561 gtgggtgtcg ctggcgtaac caatacccgg cgctgcgtaa ccgattcgtg tcgcgcgttt
    22621 ctgtcgtgtg acagaaagtt gttttcgctg ttcagtgggt cacgatgggg cgatgtcgat
    22681 cactcgtgct ggccgcccac ggttgacgac ccagcgtcgg ccgggcacga ccgcgcgcga
    22741 tgaaatcctc gacgccgcag gtgaactctt cacgacgctc ggctataccg gcacgtcgac
    22801 ccgcaccatc gcggaggcgg tcggtatcag gcaggcctcg ctctatcact acttcaagac
    22861 caaagatgac atcctgtgtg ccttgctcag ccagacggtg acgccgacgc tgatggtcgt
    22921 cgagagccta gcgggcacgg agccatccct cagcgcggcc gagcacctgc acgcattggc
    22981 gctcttcgat ggccgccagc tgttggacgg ccgctggaac ctgggtgcgc tgtatctgca
    23041 gcccgaactg cgcggtgcgc gcctcgaacc gttctggtcc gaccgggagc ggctgcggct
    23101 gcactatttg gcggtgagca aggccatcgt ggccgacacc ggcattgacg aggcggcagc
    23161 cgatctaccg ttccgactgg tggagtcgtt ggtcaacatg tggaccgcgc ggcccggccc
    23221 agaacggacg agattgccgg aacacattgc ggacgcgtgc atccgggtgc tcggcatgcc
    23281 ggacagcgtg acaccgcgac tgcgggcccg cagtcatctc gaattgaccc ggtacgccgc
    23341 cgactagtcc gctttcggca tcggaatgcc ctcgctggca gcgaactggg catcttcctc
    23401 cgatgcgagg ccgatcgata ccacggaacg gtcgaagaac acgtccgtca ggtacgccaa
    23461 cgccacggcc cacctgttga cgaaccgcgg gatggcatac aggtggtaga ggcgagtaac
    23521 ggccttggcg ggaaacccgg acagctgaat gttgagcggg ttggcgacgg cgcgccgagg
    23581 gccga
//
LOCUS       DASRJA010000542         8704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2856111,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000542 DASRJA010000000
VERSION     DASRJA010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2856111"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VFC01_24005"
     CDS             <1..1179
                     /locus_tag="VFC01_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HZN82714.1"
                     /translation="ELPWDSIDMDFMNLNQAAHGDREFGYIQSRLSTARKTVAGHVSD
                     QRTRSRIGAWTRASLATAELATLRVARFGDNMRNVAVTEGDKVEAEAHFGASVNTYPV
                     NELVDVVERVPAAEVDKLVREYEHAYRLAPELRPGGDRHTSLRHGAQIEAGLRKFLEN
                     GDFHAFTTNFEDLGGLRQLPGLAVQRLMADGYGFGAEGDWKTAVMLRAVKVMAEGLPG
                     GTSFMEDYTYDLTVGAERVLGAHMLEVCPSIADGVPSLEVHPLSIGGREDPVRLRFTA
                     APADAVILGISDVGSRFRLVANEVRVVEPTAPLPNLPVACAVWEPCPSWSVAAEAWLM
                     AGAPHHTVLTTAVGTEVLEDFATMTGTELLVIDGDTTVRGFIRELKWNDVYHHAAAGL
                     "
     gene            complement(1424..2410)
                     /locus_tag="VFC01_24010"
     CDS             complement(1424..2410)
                     /locus_tag="VFC01_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZN82715.1"
                     /translation="MTVGRVAELWRYPVKSLGGERIEHAEIGPRGVLGDRLWAVRDLG
                     RDVTASARQIPVLLTATARYVGPVSPDAGPGNVPEVEISFPDGTVLSSSDGGVHAKLS
                     ELAGRDVRLTALPPAEDTSLHKLTRAERSETMSPAWLRAAFGVADDEKLPDLSMVRVA
                     DMVNFARYSTPPGMFVDLAPVHVLSQTSLATIGAEIGTELDVRRFRPNVLLALDDPGD
                     GLPESHWTGARVTVGEVALDVMMPTVRCVVPSRAQPGFEVDRRITKAVAVRAQRCLGV
                     YCGVDSSGTVRVGDDVAVRPVKAGRKALADAGRRAKRFTLGLASGTMERLSR"
     gene            complement(2433..2960)
                     /locus_tag="VFC01_24015"
     CDS             complement(2433..2960)
                     /locus_tag="VFC01_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZN82716.1"
                     /translation="MPLYTFEGRSPTIDPTAFIAPTATLVGDVHVEAGASVWFNAVIR
                     ADFAPIIVREGANVQDCCVLHAPPGIPVDVGPGATVAHGCVVHGVHIGAQAVLANHCT
                     VLDGAVIGARSLIAAHSLVVGGTKIPDEVLVTGAPAKVRGPIAGTGAEMWVNTNPGAY
                     QDLARRYMAGMQPIS"
     gene            3060..4214
                     /locus_tag="VFC01_24020"
     CDS             3060..4214
                     /locus_tag="VFC01_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN82717.1"
                     /translation="MTTETAGIREIDTGALPDRYARGWHCLGPVNDYLDGKPHGVEIF
                     GTMLVVFADSHGDVKVLDGYCRHMGGNLAQGTIKGDEVACPFHDWRWGGDGKCKLVPY
                     AKRTPRLARTRAWHTDVRGGLLFVWNDHEGNPPQPEVQIPDIPEAASDEWTDWRWNSI
                     LIEGSNCRDIIDNVTDMAHFFYIHFGLPTYFKNVFEGHIASQYLHNVGRPDINDMGTT
                     YGEAHLDSEASYFGPSFMINWLHNSYGGFKAESILINCHYPVTQNSFLLQWGVIVEKP
                     KGLDEETTDKLARVFTEGVSKGFQQDVEIWKHKTRIDNPLLVEEDGAVYQMRRWYQQF
                     YVDVADVTPDMTDRFEIEIDTTAANEKWHVEVEENLKQQAAEKEKAEQGL"
     gene            4262..4666
                     /locus_tag="VFC01_24025"
     CDS             4262..4666
                     /locus_tag="VFC01_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82718.1"
                     /translation="MLRLYGAHDDDHEHNGASGIRSKAPNFASDPERAAAVAEATQRD
                     RERYLTSGLVSVDCRFCHVAVQVKKLGPGHTSVQWNTEASQRCAYFAEIRQSGGDSAR
                     APSCPKLADSIKHAVAEGCLEEVSSAPSPGDG"
     gene            4749..5288
                     /locus_tag="VFC01_24030"
     CDS             4749..5288
                     /locus_tag="VFC01_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HZN82719.1"
                     /translation="MAPITALQVGDLLSSKVQDRDGNLFITVEPRECRPHALEVDPPL
                     IFDLDPAATDGGHWVSDDGVVIDETAGVYHANFDANRALAETKRTIDACRDVPFTITD
                     MRDREYHFRLLPQVNSGSPDIVLWSFTASDWACDNAFVAAHNAAVRISTCAPANGYDV
                     LSLAREALKRIEKLANTTA"
     gene            complement(5292..6437)
                     /locus_tag="VFC01_24035"
     CDS             complement(5292..6437)
                     /locus_tag="VFC01_24035"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZN82720.1"
                     /translation="MSTRIDVGTVDELHASATKACGLDDFGADDDNYREALGVLLDSY
                     AREAGLTEIGSKMSRFFVRNALVARLLSEAAWKQYPQHADVVIERPIFVTGLPRTGTT
                     ALHRLLAADPRHQGLELWLAEFPQPRPPRETWSENPVFVELDARFKQAHDENPDYTGL
                     HYMTADDVEECWQLLRQSLHSVSYETLAHVPTYARWLDKQDWTRPYQRHRKNLQLIGL
                     NDAEKRWVLKNPSHLFALDALFATYPDALVIQCHRPAETIMASMCSLAQHTTAGWSTT
                     FVGETIGQDSLQTWSRGLELFNAERKKHDSAQFYDMDYFAFIADPVGELEAIYRHFGI
                     DFTDEARAAVEETHAESKKGPRAPKHTYSLADYGLTDEQVKDRFKGL"
     gene            complement(6434..7216)
                     /locus_tag="VFC01_24040"
     CDS             complement(6434..7216)
                     /locus_tag="VFC01_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN82721.1"
                     /translation="MAGLLDDKVVVISGVGPALGTTLARRCAEAGADLVLAARTVERL
                     DDVAKQITDLGRRAVSVGTDITDEAQVNNLVEETLKAYGKVDVLINNAFRVPSMKPLA
                     NTTFEHMRDAIELTVFGALRLIQGFTPALAESKGSVVNVNSMVVRHSQAKYGAYKMAK
                     SALLAMSQSLATELGEQGIRVNSILPGYIWGGTLESYFTHQAGKYGTTVDEIYKATAA
                     TSDLKRLPTEDEVASAILFMASDLSSGITGQTLDVNCGEYKA"
     gene            complement(7217..8344)
                     /locus_tag="VFC01_24045"
     CDS             complement(7217..8344)
                     /locus_tag="VFC01_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82722.1"
                     /translation="MYSQPLMEAIAEAEKLVGEAPHIESEADLLEGLQYLAGCIAACT
                     HVAFDYERDHPFLHSGTGPFTKMGLDNPDTLYFGTRVLPEHDYVVTGKRGTTTDVSFQ
                     LLGGEYTDKVVPDSETAFDDRKLDIAADGSFEWRFRPETPSQLVIREVYNDWSAQRGS
                     FAIERVDTAGTAPPPLTRELIEKRYATAGKQLVQRVKTWLQFPQWFYTNIPVNTLVAP
                     RLTPGGLATQYSSVGHFDLTPDQAIVITLPVTDAPYLGFQLGSLWYISLDYINHQTSL
                     NGTQAQADPDGKIRIVVADRNPGVTNWVETLGHAKGYLQFRWQRVSRELTEADGPTAE
                     VVEFDKVAQSLPYYEKNKISEDDWRARIAERQTQIGHRMVG"
     gene            8431..>8704
                     /locus_tag="VFC01_24050"
     CDS             8431..>8704
                     /locus_tag="VFC01_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82723.1"
                     /translation="MSAPESAGRASPPTERVVRILDFLAARPEERFGLSDLARRLGLS
                     KPTCLGIVTSLTDAGYLVRDAGDKTYRLGPSLIGERVYLRGENGGRR"
BASE COUNT         1549 a         2826 c         2790 g         1539 t
ORIGIN      
        1 gaattgccgt gggacagcat cgatatggat ttcatgaatc tcaaccaggc ggcgcacggc
       61 gatcgcgagt tcggttacat ccagtctcgg ctgtcgacgg cacgtaagac cgtcgccggg
      121 cacgtcagcg atcaacgaac ccgctcgcgt atcggggcgt ggacgcgcgc ctcgctcgcc
      181 accgccgaac tggctaccct tcgagtggca cggttcggcg acaacatgcg caacgtcgcc
      241 gtcaccgagg gcgacaaggt cgaagcagaa gcgcacttcg gtgcatcggt caacacctat
      301 ccggtcaacg agctcgtcga cgtcgtcgaa cgagtgccgg ccgccgaggt ggacaagctt
      361 gttcgcgagt acgagcatgc ctaccggctc gcccctgagc tgagacccgg aggtgaccgc
      421 cacacctcgc ttcggcacgg cgcgcagatc gaagctgggc tgcgaaagtt ccttgagaac
      481 ggcgattttc acgcgttcac taccaacttc gaggatctcg gagggctgcg ccagctgccc
      541 ggccttgccg tgcaacggct gatggccgat ggctacggct tcggcgcaga aggcgactgg
      601 aaaaccgcgg tcatgttgcg ggcggtgaag gtgatggccg aaggtctgcc cggcggcacg
      661 tcgttcatgg aggactacac ctacgacctg acggtcggcg ccgaacgcgt ccttggcgcc
      721 cacatgctgg aagtgtgccc gtcgatcgcg gacggcgtgc cgtcgctgga ggtgcacccg
      781 ctgtcgatcg gcgggcgcga agatcccgtc cgattgcggt tcacggcggc gccggccgac
      841 gcggtgattc tcgggatcag cgatgtcggt tcgcgattcc gcctggtggc caacgaagtt
      901 cgcgtcgtcg agccgacggc tccgctgcca aaccttcccg tggcatgcgc ggtgtgggaa
      961 ccgtgcccaa gctggtccgt cgccgccgag gcgtggctga tggccggggc gccccatcac
     1021 accgtgctca ccactgcggt cggaacagag gtgctcgagg acttcgccac catgaccgga
     1081 actgagctgc tggtgatcga cggggacacc accgtgcggg ggttcattcg ggagctgaaa
     1141 tggaacgacg tgtaccacca cgccgcagcg ggtctctgac cccacgtcag tcgcgcgagc
     1201 ggaagctgga ccgcttcgac cgtgacttac tcgtgttctg gctcagttgg gcgccgtatg
     1261 gcggcccgcc cactgacgaa tgcttcgttg agttcgggat ggacgtggag cgcgtgcgtg
     1321 agcgctgcat ggaagtggtc tgctcggctc gcgccgctga attcgtcgac gccgaccgtt
     1381 cactgctgct tcgcgcctct cagctgctgc tgggacccct gactcagcgc gacagtcgtt
     1441 ccatcgttcc cgaagccaat ccgagggtga accgcttcgc gcgccgaccg gcgtcggcta
     1501 gcgccttgcg gcctgctttg acgggtcgga ccgcaacgtc atcgcccacg cggaccgtgc
     1561 cgctggagtc gacaccgcag tagacgccga ggcatcgctg cgcccgaacg gccaccgcct
     1621 tcgtgatgcg gcggtcgacc tcgaatccgg gctgcgcacg gcttggtacc acacagcgaa
     1681 ccgtcggcat catcacgtcg agagctactt cgccgaccgt cacacgggcc ccggtccagt
     1741 gcgactccgg taggccgtcg cctggatcat caagcgccag tagcacattg gggcgaaatc
     1801 ggcggacgtc gagttcagtg ccgatctctg cgccgatggt cgccaggctg gtctggctca
     1861 gcacgtgcac gggcgccaag tcgacgaaca tgcccggagg cgttgagtaa cgagcgaaat
     1921 tgaccatatc tgccactcgc accatcgaga gatcaggcag cttttcatcg tcggcgacac
     1981 cgaaggcggc gcgaagccac gcgggtgaca ttgtttcgga gcgctcggcg cgggtcagct
     2041 tgtgcaggct ggtgtcctcc gccggcggca gagccgtcag cctgacgtcg cgtcccgcca
     2101 attcggagag cttcgcgtgc acaccgccgt cgctgctgga caacaccgtg ccgtcaggga
     2161 agctgatttc aacctcggga acattccccg ggccggcatc tggcgagaca ggcccgacat
     2221 atcgggcggt cgccgtcagc agcaccggta tctgccgagc cgaagccgtg acgtcacgtc
     2281 ccaagtcgcg aacggcccac agccggtccc ccagcactcc gcggggaccg atctcggcgt
     2341 gctcgattcg ttcgcccccc agcgacttga cgggatagcg ccacaattcg gcgacccgac
     2401 caacggtcat ggcgccacgg tacggcgcgc cgttaagaga tgggctgcat tcccgccata
     2461 tatcgccggg ccaggtcctg atacgcgcca ggattggtgt tcacccacat ctccgcgccc
     2521 gtgcccgcga tcggcccacg caccttggcc ggcgcgccgg tcaccagcac ctcgtcgggg
     2581 atcttcgtgc cgccgacgac cagcgagtgc gccgcaatca ggctgcgcgc gccgatcacc
     2641 gcgccgtcga gcaccgtgca gtggttggcg agcacggcct gggccccgat atgcacgccg
     2701 tgcaccacgc agccgtgcgc gaccgtcgca cccgggccga catcgacagg aatgcccggc
     2761 ggggcatgca gcacgcagca gtcctgcacg ttggcgccct cacggacgat gatcggggcg
     2821 aagtcggcgc gaatgaccgc gttgaaccac acagacgcgc ctgcctcgac gtggacgtcg
     2881 ccgacgagcg tggcggtggg ggcgatgaac gcggtcggat cgatcgtcgg cgatcggccc
     2941 tcaaaggtgt acaacggcat cgtctacata taccgcaggt aaaacgggtt tagattgcgc
     3001 ctcggggggc aaaaactgta acgtgttcta gttagagagc acagattggg aggcccgagg
     3061 tgaccaccga aaccgcaggc attcgcgaga tcgacaccgg cgcgctgccg gacaggtacg
     3121 cgcgtggctg gcactgcctt ggcccggtga acgactacct ggacggcaaa ccgcacggtg
     3181 tcgagatttt cgggaccatg ctggtcgtct tcgcggactc ccacggcgac gtcaaggtgc
     3241 tggacgggta ctgcaggcac atgggtggca acctcgcgca gggcaccatc aagggcgacg
     3301 aggtcgcctg cccgttccac gactggcgct ggggcggcga cggcaagtgc aagctggtgc
     3361 cctacgccaa gcgcaccccg cgactggcgc ggacgcgcgc gtggcacacg gacgtccgcg
     3421 gtggcctgct attcgtctgg aacgaccacg agggcaaccc gccgcagccc gaggtacaga
     3481 tcccggacat cccggaggcc gccagtgacg agtggaccga ctggcgctgg aactcgatcc
     3541 tgatcgaggg ctccaactgc cgcgacatca tcgacaacgt caccgacatg gcgcacttct
     3601 tctatatcca cttcggtctg ccgacgtact tcaagaacgt gttcgagggc cacatcgcct
     3661 cgcagtatct gcacaacgtc ggccggcccg acatcaacga catgggcacc acatacggtg
     3721 aagcgcacct ggattcggag gcgtcgtact tcggcccgtc cttcatgatc aactggttgc
     3781 acaacagcta cggcggcttc aaggccgagt cgatcctgat caactgccac taccccgtca
     3841 cccagaactc gttcttgctg cagtggggcg tcatcgtgga gaagccgaag gggctcgacg
     3901 aagagaccac cgacaagctg gcccgggtgt tcaccgaagg tgtgagcaag ggattccagc
     3961 aggacgtcga gatctggaag cacaagaccc gcatcgacaa tccgctgctg gtcgaggagg
     4021 acggcgccgt ttatcagatg cgccggtggt accagcagtt ctacgtcgat gtcgccgacg
     4081 tgacgccgga tatgaccgac cggttcgaga tcgagatcga caccacggcg gccaatgaga
     4141 agtggcatgt cgaggtcgag gagaatctca agcagcaggc tgccgagaaa gaaaaggccg
     4201 aacaggggtt gtagtgagcc gggagcgcaa cgcgccagac gtcgacgagc tagcgcggtc
     4261 gatgctgagg ctctacggcg cccacgacga cgaccatgaa cacaacggcg ccagtgggat
     4321 ccgttccaag gcgcccaact tcgcgtcgga tcctgagcgt gccgccgccg tggcggaagc
     4381 cacgcaacgc gaccgcgaac ggtatctgac ctccgggctg gtgtcggttg actgccggtt
     4441 ctgccacgtc gcggtgcagg tgaagaagct cggcccgggg catacttcag tgcagtggaa
     4501 caccgaggcg tcacagcggt gcgcgtactt cgccgagatc cggcagtccg gcggtgattc
     4561 cgcacgtgcc ccgtcgtgcc cgaagctggc cgacagcatc aaacacgccg tcgccgaagg
     4621 gtgtttggag gaggtatcca gtgcgccctc ccccggcgac ggctagcgta tcggcgctgt
     4681 tgcttggttt cctcctcgga ggctgcacgc agacggttga cacaccacaa ccgcgggccg
     4741 agccggcggt ggcgcccatt accgcgctac aggtcggcga cctgttgagc tcgaaggtcc
     4801 aggacaggga cggcaatctg ttcatcaccg ttgaaccacg agagtgcaga cctcacgcgc
     4861 tcgaggtgga tccaccgctc atcttcgacc tcgaccctgc ggcaaccgat ggtgggcatt
     4921 gggtctctga cgacggagtc gtgatcgacg agacagccgg tgtctatcac gccaacttcg
     4981 acgccaaccg cgcattggca gaaacgaagc gcaccatcga tgcctgtcgg gatgttccat
     5041 tcacgatcac cgacatgcga gaccgcgagt accacttcag gcttctgccc caggtgaatt
     5101 cgggatcgcc ggacatcgtg ctgtggtcat tcacagccag cgactgggcc tgcgacaacg
     5161 cgttcgttgc cgcgcacaat gctgccgtcc ggatctcgac gtgtgcaccc gccaacggct
     5221 acgacgtgtt atcgctggcg cgcgaagcgt tgaaacggat tgagaagctg gccaatacca
     5281 cggcgtgacg cctagagccc cttgaaccgg tccttgacct gctcgtcggt caggccgtag
     5341 tccgcaagcg aatacgtgtg cttgggcgcc cgtggcccct tcttgctctc cgcgtgggtc
     5401 tcctcgacgg cagcgcgggc ttcgtcggtg aagtcgatgc cgaagtgacg atagatcgct
     5461 tcgagttcgc cgaccgggtc ggcgatgaaa gcgaagtagt ccatgtcgta gaactgagcc
     5521 gaatcatgtt ttttccgttc ggcattgaac agctccagac cgcgcgacca agtctgtagt
     5581 gagtcctgcc caatggtctc gccgacgaac gtggtcgacc agccggcagt ggtgtgctgt
     5641 gccagcgagc acatcgacgc catgatcgtc tcggccggtc ggtggcattg aatcaccagc
     5701 gcgtccggat acgttgcgaa caatgcatcg agcgcgaaca gatggctggg attcttgagc
     5761 acccaccgct tttccgcatc gttcagcccg atcagctgaa ggttcttgcg gtgccgctga
     5821 tacggtctcg tccagtcctg cttgtccaac caccgggcgt aggtgggcac atgcgccagc
     5881 gtctcgtagg acaccgaatg cagcgactga cgcagcagtt gccagcactc ctcgacgtcg
     5941 tcggcggtca tgtagtgcag cccggtgtag tccgggttct cgtcgtgcgc ctgcttgaat
     6001 cgcgcatcaa gttcaacgaa aaccggattc tccgaccagg tttcgcgcgg cggacgcggc
     6061 tgcgggaatt ctgctagcca cagttccagg ccctgatgtc gcggatcggc ggccagcaac
     6121 cggtgcagcg ctgtggtgcc ggtgcggggt aggccggtga caaagatcgg ccgctcgatc
     6181 accacgtccg cgtgctgcgg gtactgtttc cacgccgctt cggacagcag ccgcgccacc
     6241 agagcgttgc gcacgaagaa ccgcgacatc ttgctgccga tctcggtgag accggcctcg
     6301 cgggcgtacg agtcgagcag cacgccgagc gcttcgcggt agttgtcgtc gtcggcaccg
     6361 aaatcatcga ggccgcacgc cttcgtcgca gaggcgtgca gctcgtcgac agtgccgacg
     6421 tcgattctgg tgctcatgct ttgtactccc cgcagttgac gtccagcgtc tgcccggtga
     6481 ttccgctgga cagatcgctg gccatgaaca ggatcgccga cgccacctcg tcctcggtcg
     6541 gcagccgctt caaatccgag gtcgcagcgg tggccttgta gatctcgtcc acggtggtgc
     6601 cgtacttgcc ggcctggtgg gtgaagtaac tttccagcgt tccaccccag atgtagccgg
     6661 gcagaatcga attcactcgg atgccctgct cgccgagttc ggtggccagc gactgtgaca
     6721 tcgccaagag cgcggacttc gccatcttgt aggcgccgta cttggcctgc gagtgccgaa
     6781 ccaccatcga gttcacgttg acgacggacc ccttcgactc cgccaacgcg ggggtgaagc
     6841 cctggatcag tcgcagggcg ccgaacaccg tcagctcgat cgcgtcgcgc atgtgctcga
     6901 aggtggtgtt ggccaacggt ttcatcgacg gcacccggaa cgcattgttg atcagcacgt
     6961 cgaccttgcc gtacgccttg agcgtctcct cgacgaggtt gttcacctgc gcctcgtcgg
     7021 tgatgtcggt gccgactgac acagcgcgcc tgccgagatc agtgatctgc ttggccacgt
     7081 catcgagacg ctcgacagtc cgggccgcca gcactagatc cgcacccgct tccgcgcatc
     7141 gccgcgcaag cgtggtgccc agtgccggac ccacgccact gatcacgacg accttgtcat
     7201 ccagcaagcc agccatctac cccaccatcc tgtgtccaat ttgtgtctgg cgctcggcaa
     7261 ttcgcgcacg ccagtcgtct tcggagatct tgttcttctc gtagtacggc agcgactgcg
     7321 cgaccttgtc gaactcgacc acttccgcgg tcgggccgtc tgcctcggtg agctcacgcg
     7381 acacccgctg ccaccggaac tgcaggtaac ccttggcgtg cccgagcgtc tccacccagt
     7441 tggtcacgcc ggggttgcgg tcggcgacca cgatgcggat cttgccgtcc ggatccgcct
     7501 gcgcctgagt gccgttcagc gacgtctgat ggttgatgta gtcgagcgag atgtaccaca
     7561 ggctgcccag ctggaagccc aggtaaggag cgtcggtcac gggcagcgtg atgacgatcg
     7621 cctggtccgg cgtcaggtcg aagtgcccga cggacgagta ctgggtggcc agcccgcccg
     7681 gcgtgagccg cggcgccacc aacgtgttga ccggaatgtt ggtatagaac cactgcggaa
     7741 actgcagcca agtcttgacc cgctgcacca gctgctttcc cgctgtggca taacgcttct
     7801 cgatcagctc acgagtcagc ggcggcggtg cggtgcccgc ggtgtcgacg cgctcgatcg
     7861 cgaacgagcc gcgttgtgcc gaccagtcgt tgtagacctc cctgatcacc agctgggagg
     7921 gtgtctccgg ccggaaccgc cactcgaagc tgccatccgc cgcgatgtcg agtttgcgat
     7981 cgtcgaacgc cgtttcgctg tcgggcacca ccttgtcggt gtactcaccg ccgagcagct
     8041 ggaagctcac gtcggtggtg gtgccgcgct tcccggtcac gacgtagtcg tgttcgggca
     8101 gcacgcgggt gccgaagtag agggtgtcgg gattgtcgag gcccatcttg gtgaacgggc
     8161 ccgtaccgct gtgcaggaac gggtggtcac gctcgtagtc gaacgccacg tgcgtacacg
     8221 ctgcgatgca gcctgcgagg tattgcaggc cttccaacag atcggcttcg gattcgatgt
     8281 ggggcgcttc gccgaccagc ttctcggcct cggcgatcgc ctccatcagt ggctgcgagt
     8341 acacgccctc gaaactagaa cgtgttctac caagagtcaa tatatgtacc gcagcggtac
     8401 atatatggaa cagcgaggta ccctgctctc gtgtccgccc cagagtccgc cggccgcgcg
     8461 tcgccgccca ccgaacgggt ggtccgcatc ctcgattttc tcgccgcccg tccagaggaa
     8521 cgcttcgggc tgtccgacct cgcccgcagg ctggggctga gcaagccgac ctgccttggc
     8581 atcgtcacgt cgctcaccga cgccggatac ctggtacgcg atgccggcga caagacgtac
     8641 cgactgggtc cctcgttgat cggcgagcgg gtgtacctgc gtggcgagaa cggcgggcgc
     8701 agac
//
LOCUS       DASRJA010000543        11601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4223951,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000543 DASRJA010000000
VERSION     DASRJA010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11601
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4223951"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="VFC01_24055"
     CDS             complement(<1..1408)
                     /locus_tag="VFC01_24055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82724.1"
                     /translation="MSKHARKSSSPQLRILTAAMATGTMVVASAALTGNQPEPAAPAY
                     TPTALAGLQVPTPNSLLTGSTGTLTTALTNFSGTTSSGGGPLAALTGMMLTPPSGDTT
                     SSSGPALVGPATTSGPSSSSASVPIALAGRAPTGVPTLPSDPTDPSRVPTPSPRAPAQ
                     LLPLIGNVDFVVARLIGLPGQILANPLNIIGPGGWLIGDAVTAGANGGFLIGNGADGV
                     LAGQDGGAGGIFFGNGGDGANGRPGQDGGDGGAAGLFGGNGGDGGDGGDVTARTGGTA
                     GDGGAGGSAPSGLGGDGGDGGDAGTIRIVNGERVDNSIGGPATGGNGGDGGDGGLGGG
                     DGGKGGSAATTNGDATAGSGGDGGNSNSGDGGKGGDAGLIAGTTGLGATATGGDGGNG
                     GNGGIRDGMATQGGDGGNGSNDVVSSRGDAVGGDGGDGGDAVRGSGGDGGNASAPGDF
                     AEGVFADGNATGGDGGDGG"
     gene            1589..3523
                     /locus_tag="VFC01_24060"
     CDS             1589..3523
                     /locus_tag="VFC01_24060"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN82725.1"
                     /translation="MIRGWPLVGRSEELAVIADATRPATDRARGIVLSGSAGVGKTRV
                     AREAVAGCGRRDTRRHWIVGTASARGVPLGAFADIASDFGPDPLRRVREVIDGLIGTA
                     HNGDVVIGVDDAHLLDDLSAFTVHQLVTRRLATVILTIRSGEPTPDAITAIWKDHHLE
                     RLELQPLSLPEIKSLLEYVLSGAVDSFSAQRLWRYTQGNALYLRHLLENEVSADRMTQ
                     RSGVWLWEGHPRLSPTLAELLDARIADVPRSVRDVLDALAVAEPLETDILKAVADSDA
                     LAEAESLGLISVDAGVRPASVRLAHPMLGEVRRTGSLRLQLLRGRIATELARKNSTDP
                     RDVVRRAALTLESDLAPDSEVLVAAASAAMQLGDLRLAETLAERAVAAGGGLPAKIVQ
                     AMAITWQERGADAEAVLAELADETRDPIRAHIAILRAMNFAASLGDAASAERELNAAI
                     PADDEAAQAISNVVRAFIDLVRGHSRTAADRASVSLANTPASDMAQMFLIWTLVSALG
                     DLGSIDEIESAADAGYRLAETSAEASHLRLPLATLQVIGYWLAGALTASDTMIARLRS
                     DTIDVPFQQSWRSFFTAMSAISRGDLVAAQRSLQETIAHLESGGLGQMLKAFARSSLA
                     TVTGMAGKAVDARCEYTALE"
     gene            3584..4198
                     /locus_tag="VFC01_24065"
     CDS             3584..4198
                     /locus_tag="VFC01_24065"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN82726.1"
                     /translation="MCAAEGSTTQAISITRDAAEQERRLGRPAWEVMLLQTATQFGDR
                     TAAPRLAELAGDVQGPRAAAAATHAAALAAGDGDELVEASRRYEEFGDRLAAADAAAQ
                     AVVIYQQAGSRGAAMNASAIVERLAAECQGGQTPALRAAAAPQPFTARQREIISLAAQ
                     GLTNKEIADRLTMSKRSVEGHLFRASQRVGANSREQLISILQGA"
     gene            complement(4195..4866)
                     /locus_tag="VFC01_24070"
     CDS             complement(4195..4866)
                     /locus_tag="VFC01_24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN82727.1"
                     /translation="MDDDAQPTRQQISDAVDTAGWRLILGAIYAEVRVPSLAEATEAA
                     SLAVSAVGSDGQGHLTVDIRADRAVLRLHSADVAAVTGRDIRLAHDVSGALSSRGLQL
                     APGTAQALEIAIDALDIPLVRPFWKAITGYADEPGSELNAALIDPSGRGPAIWFQQMD
                     APRPQRNRIHIDVDVPHEAASARIDAAIAAGGTLLSDAEAPAFWVLADPEGNEACICT
                     WQGRD"
     gene            complement(4866..6371)
                     /locus_tag="VFC01_24075"
     CDS             complement(4866..6371)
                     /locus_tag="VFC01_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN82728.1"
                     /translation="MSGVGGSSGSTEPTTAPLSSSVLGVAIIAITGMQLMSTLDGTIV
                     IVALPRMQAELDLSDVTKSWIITAYVLTFGGLLLLGGRIGDAIGHKRAFLSGVGVFTI
                     ASLVCGLATDGITLIVARAVQGSGAAVAAPTGLALIATTYAVGHARNQAMAVSAAMQG
                     IGSVLGLVLGGLLTVISWRLAFLINVPIGIVIIAIAVYRIGETHHERLKLDITGALLA
                     TLGCTSAVLVFTQGPPRAWVDPWVIGAAIATVVFFAAFLIVERTAEHPIVPFSVFDNR
                     NRVMTFISLFLAGGVMLTATVIIGLYVQDVMGYSALRAGICFIPFALAMGTGNVVAAR
                     IAPYIAPRWLVIGFGALVFGAMLYGSTLNRHIPYFPDLVLPLVVGGLGIGVISVVLPL
                     CAIAETGPKEIGPVSSITLMVQNLGGPVVLVVIQAVLTSRTLYLGGTTGPVKDMTPGQ
                     LDALDHGYTYSLLWVAGIAILVGIAAFWIGYSARQIATAQHTREAVEAGEL"
     gene            6537..6815
                     /locus_tag="VFC01_24080"
     CDS             6537..6815
                     /locus_tag="VFC01_24080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82729.1"
                     /translation="MTHPTASRLRITGMYRRSDHSAAMYRVSDRLHEGRAVHVSAHEI
                     ATTVSAWLAELGANSPLVDDLARAVRAGDWPTALGLGEYLSVDVTVAA"
     gene            6905..7237
                     /locus_tag="VFC01_24085"
     CDS             6905..7237
                     /locus_tag="VFC01_24085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82730.1"
                     /translation="MRTPEPLVGAVLAAAALSGAILLSAFDAHAAPKKTQVQVDCEEA
                     GGKYVRNSPSSESCCFEEALDDEDHHCKEYIAGEYVGVSRDVTVGPGPAVAQLDPLWV
                     PRAGRQPQ"
     gene            7316..8644
                     /locus_tag="VFC01_24090"
     CDS             7316..8644
                     /locus_tag="VFC01_24090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN82731.1"
                     /translation="MTALPDWHIASDGELADAAAAGDRVAFAAIYDRYADRLHDFCIG
                     MVRDRDAAADCVQEVFCTVADRLSQLNDADKLRPWLYAIARNEALRHIRQRGRERAFD
                     QVPETVSGEPGPDILAARTELADLIAEAAGGLSDRDRSVLELSYRHGLDGPEVAEALG
                     VSHASAKKMTQRLRETIERSLGALLVSRRVQNNTFGCAELAEILDGWDGKFSVLWRKR
                     IARHIEACRTCDQERRRLVNPVALLGAAPVFIPAPDWLRDRTLSRIQLTAASGDQSPD
                     GHGFPRRHVLAGVALLAVIVIAAIGATIAVLQQRDAPMTPANVTEATPSPASVAPRSA
                     VTPSSNPPPPPTEASVLVPTTSAPVQSPPPAAPATPTSPVATPSPTVNSPSPRPTTTV
                     TSTRPITTPKPAPTPPLEEPTTTATVPKPPLTQQPHTPVTKNPPTTTVIE"
     gene            complement(9057..10637)
                     /locus_tag="VFC01_24095"
     CDS             complement(9057..10637)
                     /locus_tag="VFC01_24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN82732.1"
                     /translation="MTALNDAERAAMRRDAEGGANRSLPTHVEHTPLEQSGRSPAWLP
                     SRRFIAAVVAIGGMQLLATMDSTVAIVALPKIQDELSLSDAGRSWVITAYVLTFGGLM
                     LLGGRLGDTIGRKRTFIAGVALFTIASILCGLAWNEATLVTARLLQGVGAAIASPTAL
                     ALIATTFPKGPARNAATAIFAAMTGVGSVMGLIVGGALTEVSWRWAFLINIPIGLLMI
                     QLARTTLRETHRERLKLDAAGAILATLACTFAVFGFSMGPEHGWLEPLTIGSGVAALA
                     FLIAFLYVERTAENPVVPFELFKDRNRVATFAAVFLAGGVMFTLTVLIGLYVQDIMGY
                     SALRAGVGFIPFVIALGIGLGLSSHLVSLFPPRVLVIAGGVLVLGAMLYGSTLDAGIP
                     YFPNLVLPITVGGFGIGMIVVPLTVSAIAGVGFDQIGPVSAIALMLQNLGGPVVLAVI
                     QAVITSRTLYLGGTTGPVKNMDSAQIHALDQGYTYGLLWVAAVAVIVGGVALFIGYTS
                     AQVAHAQEVKDAIDAGEL"
     gene            complement(10671..>11601)
                     /gene="cobA"
                     /locus_tag="VFC01_24100"
     CDS             complement(10671..>11601)
                     /gene="cobA"
                     /locus_tag="VFC01_24100"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZN82733.1"
                     /translation="AEAERRHIFCVRADVAREGTAVTPASFEYEGLSVGVLAGGEHRR
                     SAAIRSAIHEALQQGLITAETPGVVHGGVALVGGGPGDPELITVRGRRLLAHADVVVA
                     DRLAPQELLAELPPQVEVIDAAKIPYGRAMAQDAINDVLIDRAKAGKFVVRLKGGDPF
                     VFARGYEEVLACADAGIPVTVVPGVTSAIAVPALAGVPVTHRAVTHEFVVVSGHVAPG
                     HPESLVNWNALAAMSGTIVLLMAVERIELFTKALLDGGRPADTPVLVVQHGTTTAQRT
                     LRATLDDAPERIREEGIRPPAIIVIGSVAAFGG"
BASE COUNT         2036 a         4014 c         3756 g         1795 t
ORIGIN      
        1 cgccgccgtc gccgccgtcg ccgccggtgg cgttgccgtc cgcgaaaacc ccttctgcga
       61 aatcaccggg tgcggacgcg ttgccaccgt cgccgccgct accccgcacg gcgtcgccgc
      121 catcgccgcc gtcgccgccg accgcgtcac ccctcgagct gacgacgtcg ttgctgccgt
      181 tgccgccgtc gccgccttgc gtggccatgc cgtcgcgaat gccgccgtta ccgccgttac
      241 cgccgtcgcc gccggtcgcg gtggcgccca agccggtggt gcctgcgatc agcccggcgt
      301 ctccgccctt gccgccgtca ccagaattgc tgttgccgcc atcgccgccg ctgccggcgg
      361 tggcgtcacc gttggtggta gcggcgctgc cgcccttgcc cccgtcgccg ccgccgagcc
      421 cgccgtcgcc accgtcaccg ccattgccgc cggtcgcggg ccctccaatg gagttgtcga
      481 cccgttcgcc gttgacgatt ctgatcgtgc cggcgtcccc gccgtcgccg ccgtctccgc
      541 caaggccgct tggtgccgaa ccgccagcgc cgccgtcgcc cgccgtgccg ccggtccttg
      601 cggtcacgtc gccgccgtcg ccaccgtcac cgccgttgcc gccgaacagc ccggcggctc
      661 caccatcgcc gccgtcttgg ccgggcctgc cgttggcacc gtcgccgccg ttgccgaaga
      721 agatgccgcc cgcaccgccg tcctggcccg ctaacacgcc gtcggcgccg ttgccgatga
      781 ggaagccgcc attggcgcct gccgtgacgg cgtcaccgat gagccatcca cccggcccga
      841 tgatgttcag cgggttggcc aagatctggc ccgggagccc tatgagacgg gcgacaacga
      901 agtccacgtt gccgatcaat ggcaagagtt gggccggcgc cctcggcgac ggagtaggga
      961 cgcggctggg gtcggtcggg tcgctcggca aggtcggcac gccggtgggc gcccggccgg
     1021 ccagcgcgat cggcaccgac gcgctcgacg acgagggccc gctggtggta gccgggccga
     1081 ccagcgcagg cccactcgac gaggtcgtgt cgccgctggg cggcgtgagc atcattccag
     1141 tcagcgcggc aagcgggcca ccacccgacg aggtggtgcc gctgaagttg gtcagggcgg
     1201 tggtcaaggt gccggtggag ccggtcagca gactgttcgg cgtgggaacc tgcagcccgg
     1261 ccagcgccgt gggcgtgtac gccggcgcgg cgggttccgg ttggtttccc gtcagagcgg
     1321 cactggcgac gaccattgtg ccggtagcca tcgccgcggt gagaatccgg agctggggcg
     1381 aagaagactt ccgtgcatgc ttcgacatgg gaggatccct ttccgaagtt gattgccgtt
     1441 cgcgggatgt ttgccagcaa cctaagtccg tcgcggccga acgactggcg taggcgacta
     1501 ccccactctt gccagatgga ctacgcctca tgaggtatcg gttggttgaa aacggaatct
     1561 ggacgccagt actagttaga atttgccggt gatccggggc tggccgctag tcgggcgatc
     1621 cgaggagcta gccgtgatcg ccgacgccac ccggccagcc accgaccgcg cccgcgggat
     1681 cgtgctatcc ggctccgccg gcgtcggcaa gacccgagtg gcgcgcgaag ccgtggccgg
     1741 ctgtggccgc cgtgacacgc gccggcactg gatcgtcggc accgcatcgg cgcgcggtgt
     1801 ccccctgggc gcattcgccg acatcgccag cgacttcggc cccgacccct tgcgacgggt
     1861 acgcgaggtg atcgacggcc tcatcggtac cgcccacaac ggcgacgtgg tcatcggtgt
     1921 cgatgatgcg catctcctgg atgacctgtc ggcgttcaca gttcaccagc tcgtcacacg
     1981 tcgcctggcc accgtcatcc tgacgatccg ctcgggagaa ccgacaccgg acgccatcac
     2041 cgcgatctgg aaagaccatc atctggaacg ccttgagcta caaccactat cgctgccgga
     2101 gatcaagagt ctgctggagt acgtgctcag tggtgcggtg gactccttta gcgcacaacg
     2161 actttggcgc tacacccaag gcaacgcgct gtatcttcgt catctcctcg aaaacgaggt
     2221 gagtgccgat cggatgacgc aacgctcagg cgtgtggctg tgggaggggc atcccaggct
     2281 gtcgccgacg ctggcagagt tgcttgatgc gcggatcgcc gacgtgccga ggtctgtccg
     2341 cgatgttctc gacgccctcg ccgtggccga gccgttggag accgacatct tgaaagcagt
     2401 cgccgattcc gacgcactgg ccgaggccga atcattgggc ctgataagcg tcgacgccgg
     2461 cgtgcgacca gcgtctgtgc ggctcgctca cccgatgctt ggcgaagtca ggcgcaccgg
     2521 ctcgctgcgg ctgcaattgc ttcgcggtcg aatagccacc gagctcgctc ggaagaactc
     2581 aaccgatcct cgagacgtgg tgcgacgcgc ggcgctcacc ttggagtccg atctcgcacc
     2641 tgacagtgag gtccttgtcg ccgcagcgtc ggccgccatg cagctcggtg atctacgact
     2701 cgcagaaacc ctcgccgagc gggccgttgc cgccggtggc ggtttgccgg cgaagatcgt
     2761 ccaagcgatg gcgatcacct ggcaggagcg cggagccgac gcggaggcgg tcctcgcgga
     2821 actcgccgac gagacgcgcg acccgatccg agcccacatc gcgatcctcc gcgcgatgaa
     2881 ttttgccgcc agcctcggcg atgccgcaag tgccgaacga gaactcaatg cggcgatacc
     2941 cgccgatgat gaagcggcac aagcgatctc caacgtcgtg cgggcgttca tcgatctggt
     3001 gcgcggccac tccaggaccg ccgccgatcg agccagcgtt agcctcgcca atacaccggc
     3061 gagcgatatg gcgcagatgt tcttgatctg gacgttggtc agtgcgctgg gcgatcttgg
     3121 atccatcgac gagatcgaat ccgcggcgga tgccggctac cgacttgctg agacctccgc
     3181 tgaagcctcg catctgcgcc tgccgctagc gacactgcaa gttatcggat actggctcgc
     3241 cggtgcgctg accgcctcgg acacaatgat tgcgcggctt cgaagcgaca cgatcgacgt
     3301 tccctttcag cagtcctggc gtagcttttt caccgccatg tcggcgatca gccgaggaga
     3361 tctcgttgcg gcgcaacgtt cgctgcaaga aacgatcgct catctggaaa gcggcgggct
     3421 cggccagatg ctgaaggcct tcgcgcgatc gtcgttggca accgtgaccg gcatggccgg
     3481 caaagcagta gacgcgcgat gcgagtacac ggctctcgag tagtgggacc gggaccccga
     3541 tgcatgcaag tgggactcgg cgaaatccat cgcggaggcc tgggtgtgtg ccgccgaagg
     3601 ctctaccaca caggcgatct cgatcactcg cgacgccgcg gagcaggagc gtcgactggg
     3661 ccgacccgcc tgggaagtca tgcttctgca gaccgcgaca caattcggcg accgcaccgc
     3721 tgctcccaga ctggccgaac tggccggcga tgttcaaggt ccccgcgcgg ctgctgccgc
     3781 aacccacgcc gccgcgctgg cggccggaga cggtgatgag ttggtcgaag catcccgccg
     3841 ttacgaagaa ttcggtgacc gactcgccgc cgccgacgcc gctgcccaag cagtcgtcat
     3901 ataccaacag gccggatcgc ggggcgcggc aatgaacgcc tccgccatcg ttgagcgcct
     3961 ggccgccgag tgccagggcg gccaaactcc tgccttgcgg gccgcggcgg cgccgcagcc
     4021 gttcacggcc cgtcagcgcg aaatcatatc ccttgccgca caagggttaa cgaataagga
     4081 gatcgccgat cggctaacca tgtcgaagcg ttctgtcgaa ggtcacctct tccgcgcatc
     4141 tcaacgcgtc ggagccaaca gccgcgaaca gctgatctcg attttgcaag gagcttaatc
     4201 ccggccttgc caggtgcaga tgcaggcctc gttgccttcg ggatcggcca gcacccagaa
     4261 ggccggcgcc tccgcgtcag acaacagcgt gccgcccgcg gcgatcgccg catcgatccg
     4321 ggcgctggcc gcctcgtgcg gcacgtcgac atcgatgtgg atccgattgc gctgcgggcg
     4381 tggcgcatcc atctgttgaa accagattgc gggtccgcga ccggatgggt cgatcagcgc
     4441 cgcattcaat tcggatccgg gttcgtcggc gtatccggtg attgccttcc aaaacggccg
     4501 caccagcggg atgtcgagcg cgtcaatggc gatctcgagt gcctgcgcag tgcccggcgc
     4561 aagctgcaat ccccgtgagg acagcgcccc ggagacgtca tgcgccagcc gaatgtcgcg
     4621 cccggtgacg gcggcgacgt ctgcagaatg cagccgcagc acggcccggt ccgcgcggat
     4681 gtcgacggtc aaatgtccct gcccgtctga gccgacggcc gaaaccgcca gcgacgctgc
     4741 ctccgtcgct tccgccaagg acggcacccg aacttcggcg tagatggccc caaggatcag
     4801 gcgccagccg gccgtgtcga ccgcgtcgga aatctgttga cgggtgggct gcgcgtcgtc
     4861 gtccatcaca gctccccggc ttcgacggct tcacgcgtgt gctgcgcggt ggcgatctgg
     4921 cgcgccgagt agccgatcca gaacgcggcg atgcccacga gtatcgcgat gcccgcgacc
     4981 cacaacagcg agtaggtgta gccgtggtcg agcgcgtcga gctggccggg cgtcatgtcc
     5041 ttcaccggtc cggtcgtgcc acctagatac aacgttcgcg acgtcagcac ggcctggatg
     5101 accacgagca ccactggccc gcccaggttt tgcaccatca gcgtgatcga cgaaaccgga
     5161 ccgatctcct ttggccccgt ctccgcgatc gcgcacagcg gtagcacgac cgagatcacg
     5221 ccaattccca agccccccac cacaagtggc aggacgaggt cggggaagta cgggatgtgg
     5281 cggttcaatg tcgagccata cagcatggcg ccgaacacca atgcgccgaa gccgatgacg
     5341 agccagcgcg gtgcgatata cggcgcgatg cgggcggcca ccacatttcc cgttcccatc
     5401 gcgagcgcaa acggaatgaa gcaaatgccc gcccgcagcg ccgaataccc catcacgtcc
     5461 tgcacgtaga gcccgatgat caccgtcgcc gtcagcatca cgccgccggc cagaaacagc
     5521 gagatgaacg tcatcacgcg gttgcggttg tcgaacaccg agaacggcac gatcgggtgc
     5581 tctgcggtgc gctcgacgat gaggaacgcg gcgaagaaca cgacagtggc gatcgccgca
     5641 ccgatgaccc aagggtcgac ccaggcacgc ggtgggcctt gggtgaagac gaggacggcc
     5701 gaggtacagc cgagggtggc cagcagcgcg ccggtgatgt cgagcttgag gcgctcgtgg
     5761 tgcgtttcgc cgatccggta gaccgcgatc gcgatgatga cgatgccgat cggcacgttg
     5821 atcagaaacg ccaaccgcca ggagatcacg gtcaacaagc caccgagcac aaggccaagc
     5881 accgacccga tgccctgcat cgcggccgac accgccatcg cttggttgcg ggcgtgtccg
     5941 acggcgtagg tggtcgcgat cagcgcgagg ccggtagggg cggccaccgc cgcgccgctg
     6001 ccctggacgg ccctggcgac gatcaacgtg atgccgtcgg tggccaggcc gcagaccaac
     6061 gaagcgatcg tgaacactcc gacgcctgac agaaacgccc gcttgtgccc gatcgcgtcg
     6121 ccgatgcggc cacccagcag tagcagcccg ccgaaggtca gcacgtaggc ggtgatgatc
     6181 cagctcttgg tgacgtcgga gaggtccagc tcggcctgca tccgcggcag cgcgacgatg
     6241 acgatggtgc cgtcgagcgt cgacatgagc tgcatgccgg tgatcgcgat gatcgcaacg
     6301 ccgagcaccg acgacgacag aggtgcggtg gtgggctcag tcgagcctga agaaccaccg
     6361 acgccagaca tgggttgcca gcctacccag cgacaagttc cgcggagtca gtagcgctcc
     6421 gtgcgtgcga acatgccatc gaaagttgac gaaaaagtgt ctgatcggcg cggcctggct
     6481 gctcttacct gtgagaagag ctctacggag ccgccagcca cgagttagga cacgagatga
     6541 ctcatccaac cgcgagtcgc ctccgaataa cgggcatgta ccgacggtcc gatcacagcg
     6601 cggcgatgta tcgcgtcagt gatcgcctgc acgaaggacg cgccgtccac gtgtcggctc
     6661 acgagattgc gaccaccgtg tcggcctggt tggcggagtt gggcgcgaac agcccattgg
     6721 tcgacgacct ggcacgcgcg gtgcgcgccg gtgactggcc cacggctctc ggcctcggcg
     6781 aatacctctc cgtcgatgtg acggtggcgg cctgaactca aaagtgtccc cgcggcgcga
     6841 tgaacgtgct cataccggtg tcactggccc acccgggccg ctcatcaccg aaaggaagct
     6901 cgccatgcgc acacccgaac cccttgtggg agccgttctc gccgccgccg ccctctccgg
     6961 cgccatcctg ttgagtgcat tcgacgctca cgcggccccg aaaaaaaccc aggtccaggt
     7021 cgactgcgag gaagccggcg gtaaatacgt ccgcaacagt ccgagttcgg agtcatgctg
     7081 cttcgaggaa gccctcgacg acgaggatca ccactgcaag gaatacatcg ccggcgagta
     7141 cgtcggggta tcccgcgacg tcaccgtcgg ccccggcccc gcggtggcac agcttgatcc
     7201 gttgtgggtg ccgcgtgctg gccgacaacc gcaataaccg cgtcaattac cgccccagtg
     7261 ccccggtgtc ggcggatgat ccgtactacg agtcgatccg caggggagcg atccgatgac
     7321 cgcgctgccc gactggcaca ttgccagcga cggtgagctg gcggatgccg ccgccgccgg
     7381 ggatcgggtc gcgttcgccg cgatctatga ccgctacgcc gaccgactgc acgacttctg
     7441 catcggcatg gtgcgggatc gcgacgccgc ggccgactgt gtccaagagg tgttttgcac
     7501 cgtggctgac cgcctatccc aattgaacga tgccgacaag ttgcgcccgt ggctgtatgc
     7561 gatcgcccgc aacgaggcgc tccgccatat tcgccagcgg ggtcgcgaac gagcctttga
     7621 ccaagtgccc gaaacggttt cgggcgaacc aggtcccgac atcctggccg cccgcaccga
     7681 gctcgccgac ctgatcgccg aggccgccgg cgggttgtcc gaccgagatc gttcggtgct
     7741 cgagttgtcc taccgtcacg gccttgacgg gcctgaagta gcggaggcgc tgggtgtgag
     7801 ccatgccagc gccaagaaga tgacgcaacg gctgcgcgag acgatcgaac gttcccttgg
     7861 tgccttgctg gtatcgcgac gcgtccagaa caacaccttc ggttgcgccg agctggcgga
     7921 gatcctcgac gggtgggacg gaaagttctc ggtgctgtgg cgcaagcgga tcgcccgcca
     7981 catcgaagcg tgccgcacgt gcgatcagga gcgccgccga ctggtcaatc ccgtggcgct
     8041 gctcggcgcc gcaccggtat tcatacctgc ccccgattgg ctgcgcgatc gcacactcag
     8101 ccggattcag ctcacggcgg cgagcggtga ccaatcaccc gacgggcacg ggtttccgcg
     8161 tcgccatgtt ctggctgggg ttgcgctgtt ggctgtcatc gtgattgccg cgatcggggc
     8221 gacgatcgct gtgctgcagc agcgcgacgc gccgatgacg ccggccaatg tcaccgaggc
     8281 cacgccgtca ccggcgtcgg tcgccccgcg cagcgcggtg acgccgtcaa gcaacccgcc
     8341 accaccgccg acagaagcca gcgtcctcgt acccaccacc tccgccccag tgcagtcgcc
     8401 accacccgcc gccccggcca cgccgaccag cccggtagcc acaccctcgc ccacggtcaa
     8461 ctcaccgtct ccgcgcccga cgaccacggt cacctccacc cgtccgatca ccacgccgaa
     8521 gcctgccccg acgccaccgc tcgaagagcc cacgaccaca gccacagtcc cgaaaccccc
     8581 actgacgcaa caaccgcaca cgccagtgac caagaaccca ccgaccacca cggtcatcga
     8641 ataagccctg cgcaaaagtg tccccatggc aggtgctgcc tgctcctacc gctgtcagtg
     8701 gcccgacccg gccaccacca gtaccgaagg agcacgacat gacaggtttc gcgttgcgca
     8761 ggttggtatt cgccgcggtg attgcactgg gcgccagcgc actagcagat cccgccatcg
     8821 cgttcgcaga gcgagagtgg gatatcggga cctacgacag ttgcctgacg gcccgccttc
     8881 tggaccacga tctaagactt gccgactatg aagcttgctg catcgagtct ggcggagttt
     8941 ggaacgacat gcgcgctgag tgtggagctc ctccccaccc tgactaccag cgcacatcgc
     9001 cgccgcgggc ggctctgccg gcacagacca ctgcgccttt cagccggtga ttcgaactag
     9061 agctcgccgg cgtcgatcgc gtccttgact tcctgcgcgt gggcgacctg tgccgaggtg
     9121 tagccgatga acaacgccac gccgccgacg atcaccgcga ccgcggccac ccagagcagg
     9181 ccgtaggtgt agccctggtc gagcgcgtgg atctgcgcgc tgtccatgtt cttcaccggg
     9241 ccggtggtgc cgcccagata cagggtgcgc gacgtgatga cggcctgaat cacggccagc
     9301 acgacggggc cgccgaggtt ctgcagcatc agcgcgatcg ccgagacggg gccgatctgg
     9361 tcgaatccca cacccgcgat cgccgacacg gtcaacggga cgacgatcat gcctatgccg
     9421 aagccaccga cggtgatcgg cagcaccagg ttcgggaagt acgggatgcc tgcgtcgagc
     9481 gtcgagccgt acagcatcgc gccgagcacc agcacaccgc cggcgatcac cagcacccgc
     9541 ggtgggaaca gcgacaccag atgcgatgac aggccgaggc cgatgccgag cgcgatcacg
     9601 aacgggatga agccgacgcc tgcgcgtaat gcgctgtagc ccatgatgtc ctgcacgtac
     9661 agaccgatca gcacggtcag cgtgaacatc acgccgccgg ccaggaagac ggccgcgaag
     9721 gtcgcaacgc ggttgcggtc cttgaacagt tcgaacggca cgacggggtt ctcggcggtg
     9781 cgctcgacgt agaggaatgc gatcaggaag gccagcgcgg ccacgccgga accgatcgtc
     9841 agcggctcga gccagccatg ctcggggccc atcgagaagc cgaacaccgc gaacgtgcag
     9901 gcgagcgtcg caaggatggc accggcagcg tcgagcttga gccgctcacg gtgcgtctcg
     9961 cgcagtgtgg tgcgagccag ctggatcatc agcagcccga tcgggatgtt gatcaggaac
    10021 gcccaccgcc acgacacctc ggtcagcgca ccgccgacga tcaagcccat caccgagccg
    10081 acgccggtca tcgcggcgaa gatcgcggtg gccgcgttgc gcgcgggacc ctttgggaag
    10141 gtggtggcga tcagcgccag agcggtcggt gacgcgatcg ccgcgccgac accctgcagc
    10201 agccgggcgg tgaccagtgt cgcctcattc cacgccagac cgcacaggat cgaggcgatg
    10261 gtgaacagcg cgacgcccgc gatgaaggtc cgcttgcggc cgatggtgtc gcctaggcga
    10321 ccacccagca gcatcagccc gccgaacgtc agcacatagg ccgtgatcac ccagctgcgg
    10381 cccgcgtcag acaggctcag ctcgtcctga atcttaggaa gcgcgacgat ggcgacggtg
    10441 ctgtccatgg tggcgagcag ctgcataccg ccgatcgcga cgaccgcggc gatgaaccgc
    10501 cgcgacggca gccaagccgg ggacctgccg ctttgctcca acggcgtgtg ctccacgtgt
    10561 gtggggagcg accggttagc cccgccttcg gcgtcgcggc gcatcgcagc gcgctctgcg
    10621 tcgttgagag cggtcatagc cggttacctt acagtaatct taagaaccct ttaaccgccg
    10681 aaagccgcga cagagccgat cacgatgatc gcgggaggcc ggatgccctc ttcccgaatg
    10741 cgctccggcg cgtcgtcgag agtggcgcgc aaagtccgct gggccgtcgt ggtgccatgc
    10801 tgcaccacca gaaccggcgt atccgcaggt cggccaccgt ccagcagcgc tttggtgaac
    10861 agctcgatgc gctcgacggc catcagcaac acgatggtgc ccgacatcgc ggccagcgca
    10921 ttccaattca ctaacgattc ggggtgaccg ggcgcaacat ggccgctgac caccacaaac
    10981 tcgtgcgtga cggcgcgatg ggtgaccgga acaccggcaa gcgcgggcac ggctatggca
    11041 ctcgtcacac ccggcacaac cgtgacagga atcccagcat ccgcgcacgc gagcacctct
    11101 tcgtagccgc gcgcgaacac gaatggatcg ccgcccttga ggcgcactac gaacttgccc
    11161 gccttggccc tgtcgatcag cacgtcgttg atcgcgtcct gggccatcgc ccgcccgtac
    11221 gggatcttgg cggcgtcgat cacctcgacc tgcggcggca gctcggccag cagttcctgc
    11281 ggggccagcc ggtctgcgac gacgacgtcg gcatgtgcca gcagccgtct gccccgcacg
    11341 gtgatcagct ccggatcgcc cggtcccccg ccgaccaacg cgacgccgcc gtgcacgacg
    11401 cccggcgtct cggccgtgat caggccctgt tgcagcgctt cgtggatggc cgaccggatc
    11461 gcggcggacc ggcggtgctc accgccggcc agcaccccga ccgaaagccc ctcgtactcg
    11521 aacgaggcgg gtgtcacggc ggtgccctcg cgggccacgt cggcgcgtac gcagaagatg
    11581 tggcggcgct cggcctcggc g
//
LOCUS       DASRJA010000544         4546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3671499,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000544 DASRJA010000000
VERSION     DASRJA010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3671499"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="VFC01_24105"
     CDS             <1..1079
                     /locus_tag="VFC01_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZN82734.1"
                     /translation="SIVVRPEPMESGSYTASSRLQAAGLLGAIKLFEGAAQVVPLPKP
                     PQPIVIADYGASTGHNSLLPIGAAIAVLRKRTRPEHSILVAHTDVAENDFTVMFQTLE
                     EDPDSYLKKDAASFASAIGRSFFTQILPSNSVNLGWNSWAIQWLGRVPTAVPDHLVAA
                     YSGDENVKAAYAKQGAFDWHEFVAFRGRELCPGGRLVVLTTATGEDGEFGHRPLFSGL
                     LAELADLVAQELVTEDEVHRMCIPIISRRAADFLSPFAPKGRFERVEIEHLEVFDAED
                     RFWTQYQVDRDAKAFGAQWSAFARASVFPTMASALDGGRADPRCAEFMNRLEAGVAAR
                     MAAEPEEMQIPLAQLVLWKRPKAR"
     gene            complement(1080..2042)
                     /locus_tag="VFC01_24110"
     CDS             complement(1080..2042)
                     /locus_tag="VFC01_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82735.1"
                     /translation="MHTVAVLALPDTVAFDLSTPVEVFGRVALPSGEPGYRVLVCGAE
                     PVVSAGPLRIATDHGVDALAAADTVVVPGRNDVTVGTPDDVLYALTSASAAGTRIASI
                     CSGAFTLAEAGLLDGKRATTHWVAADLFRAAFPAVNLDPDVLYVDEAQVLTSAGASAG
                     LDLCLHMVALDYGAAVAADAARLAVAPLHRSGGQAQFILRNTAPAKHIAERTELDDVL
                     AWIEQEAHRDLTLRDIAARAAMSVRTLNRRFQAETGQTPMQWVTGVRVRHAQQLLECT
                     AYGVEKIGRDVGFSSPANFREQFRRLAGVSPLNYRNTFRDRMAG"
     gene            complement(2090..3772)
                     /locus_tag="VFC01_24115"
     CDS             complement(2090..3772)
                     /locus_tag="VFC01_24115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HZN82736.1"
                     /translation="MTTGAEVVIDRLIAGGVDVCFANPGTSEMHFVAALDSAPQMRAV
                     LCTFEGVATGAADGYARIAGKPAATLLHLGPGMANGLANLHNARRACSPMVNVVGDHA
                     TYHQKLDAPLQSDIESLGNWLHGTVHRPPSAAKLAPTVDAAIAAASFGRIATLVLPAD
                     LSWSEAESDDAEAPDVPLTTREREIVRLVEQGYTTREVAEKLTISIRTVEGHMYRVTQ
                     KLGPPSQWDPGMHQPGDTDVRGAVDVLKSHGEKAVLLLGGAATDAPGIRAAARISAAT
                     GARALVETFPARLARGKGVPNIPRLGYFAEMAQEQLMGTRHLILVGARSPVAFFAYPN
                     MPGDLVPAGAKVHNVPPGRLEGVADSLDASEVSDPPSSLPELPTGPLRSDNWGSVIGA
                     LLPENAIISDEANTSGLLLPDATAASPPHDVLTLTGGAIGQGLPVAVGAAIAAPHRPV
                     IALQADGSALYTISALWTMAREQLDVTVVILNNRAYAILQFELTRVGASATGPKAQSL
                     LDLSNPDIDFVAIAEGLGVPAKRATTAEELATHFGAALAEPGPHLIDAVLAR"
     gene            complement(3825..>4546)
                     /locus_tag="VFC01_24120"
     CDS             complement(3825..>4546)
                     /locus_tag="VFC01_24120"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN82737.1"
                     /translation="GFYDAPDTAALERQRKFHQRRMAKSKLIAARQGRPFWESKRYQA
                     HKAAAADLSAQQARVREDFAHKLSTRLVRGHDFIGIEKLSLDRMTRRGKGAGAAQKRG
                     LNRALRLAAVGKLAKFIEYKANLAEVPFIEVPAAYTSQRCHQCGHTCKGNRESQAVFR
                     CLSCAWTGNADTNAAVNIREEALTRWTARTEQLAGTSGEKPGGITPVSQAGSKSKTDP
                     LTGPAPGPAASAMNRKPPNAA"
BASE COUNT          724 a         1480 c         1543 g          799 t
ORIGIN      
        1 ccagcatcgt cgtgcgcccg gagccgatgg agagcggatc gtataccgct agctcccggc
       61 tgcaagccgc tggattgttg ggagcgataa agcttttcga aggcgccgcc caagtcgtcc
      121 cgctgccgaa gccaccgcaa ccgattgtca tcgccgacta cggagcgtcc actggacaca
      181 actccctgct gccgatcggt gcggcaatcg ccgtgctgcg caagcgcacc cggcccgagc
      241 actcgatcct tgtggcacac accgacgtgg ccgagaacga cttcactgtc atgttccaga
      301 cgctcgagga ggaccccgac tcgtacctga agaaggacgc ggcgagcttc gcctcggcga
      361 ttggccgttc cttcttcacc cagatcctgc cgtccaacag cgtgaacctg ggctggaact
      421 cctgggcgat ccagtggctc ggccgggtac ctactgctgt tcccgatcac ctggtggcgg
      481 cgtacagcgg cgacgaaaac gtgaaggcgg cctacgccaa gcagggcgcg ttcgactggc
      541 atgagttcgt ggcgttccgc ggccgcgagc tgtgcccggg cgggcgcctc gtggtgttga
      601 cgacggccac cggtgaggac ggcgaattcg gccaccggcc gttgttcagc ggcttgctcg
      661 ccgagctcgc cgacttggtg gcgcaggagt tggtgaccga ggacgaggtg caccgcatgt
      721 gcatcccgat catctcccgt cgtgccgctg attttctgtc accgttcgcg ccgaagggtc
      781 gattcgagcg cgtggagatc gagcacctcg aggtattcga cgccgaggac cggttctgga
      841 cgcagtacca agtcgaccgc gacgcaaagg ctttcggtgc gcagtggtcg gcgttcgcga
      901 gggcatcggt gttcccgacc atggcgtccg cgctggacgg tggtcgtgcc gacccgcggt
      961 gtgccgaatt catgaaccgg ttggaggcgg gcgttgccgc gcggatggcg gccgagccgg
     1021 aggagatgca gattccgctg gcgcagctgg tgctgtggaa acggcccaaa gcgcggtaat
     1081 cagcccgcca tccgatcgcg aaaagtgttg cggtagttca gcggagatac accggcgagc
     1141 ctgcggaact gctcgcggaa gttggcgggc gagctgaatc cgacgtcgcg gccgatcttt
     1201 tcaacgccgt acgccgtaca ctccagcagc tgttgagcgt gtcggacccg cacacccgtc
     1261 acccactgca tcggcgtttg gcccgtctcc gcttggaatc ggcggttcag cgtgcggacg
     1321 ctcatcgcgg cccgcgcggc gatgtcacgc agggtcaggt ctcggtgtgc ctcctgctcg
     1381 atccacgcca gcacatcgtc gagttcggtg cgttcggcaa tgtgcttggc gggagccgtg
     1441 tttcgcagaa tgaactgcgc ctgtcccccg ctgcggtgca gcggtgccac agcgagccgt
     1501 gcggcatcgg cggcgacggc cgcaccgtag tccagcgcga ccatgtgcag gcacagatcc
     1561 aagccggccg acgcgcccgc cgatgtcagc acctgggcct cgtcgacata gagcacgtcg
     1621 gggtcgagat tcaccgcggg gaaggccgcg cggaatagat cggccgcaac ccagtgggtg
     1681 gtcgcgcgct tcccgtcgag caggccggcc tcggcgaggg tgaacgcgcc cgagcagatc
     1741 gacgcgatcc gggtgcccgc ggcagacgcg gacgtgagtg cgtacagcac gtcgtcgggc
     1801 gtgccgacgg tgacgtcgtt gcggccggga acgacgaccg tgtcggcggc cgcgagcgcg
     1861 tcgacgccat ggtcggtggc gatgcgcaac ggccccgcgc tgacgacggg ttcggcgccg
     1921 cacaccagca cgcggtaccc cggttcaccg gacggcagcg ctacccgacc gaacacctca
     1981 acgggggtgc tcaagtcgaa cgcgaccgtg tccggcaggg cgaggaccgc gaccgtatgc
     2041 acgagtgcga cagtagcagt cgccgtggca atatcgtggc gcctggtggc tatctggcca
     2101 gcaccgcgtc gatgaggtgg gggccgggct cagccagcgc ggcgccgaaa tgggtcgcga
     2161 gttcttccgc ggtggtcgcg cgtttggcgg gaaccccgag cccttcggcg atcgcgacga
     2221 agtcgatgtc cggattggag agatcgagca aggactgcgc cttggggccg gtggcagagg
     2281 cgccgactcg cgtcagctcg aattgcagga tcgcgtatgc gcggttgttg aggatgacga
     2341 cggtgacgtc gagttgctcg cgggccatcg tccacagcgc cgaaattgtg tacagcgcac
     2401 tgccatcggc ttgcagcgca atcaccggtc ggtgcggtgc ggcaatcgcg gcgccgacgg
     2461 ccaccggcag gccctggccg atcgcgccgc cggtcagggt gagcacatcg tgcggcggcg
     2521 acgcggcggt cgcgtcgggc agcaacaggc cgctggtgtt ggcctcgtcg gagatgatcg
     2581 cgttctcggg cagcagcgcc ccgatcaccg acccccaatt gtcggatctc agcggcccgg
     2641 tgggtagctc cgggagactg gacggaggat cggaaacctc tgaggcatcg aggctgtcag
     2701 cgactccctc cagccgtccg ggcgggacat tgtgaacctt cgcacccgcc ggcaccaaat
     2761 cacccggcat gtttggatac gcaaaaaacg ctaccggtga ccgagcaccg acgagaatca
     2821 aatgtcttgt gcccatcagc tgttcctgcg ccatttcggc gaagtagccg agacgcggaa
     2881 tgttcggcac acccttgccc cgtgccagcc gcgcgggaaa tgtttcaacc agcgcccgcg
     2941 ccccggtggc agccgaaatc cttgcggccg cgcgtattcc gggcgcatcg gtggcggcgc
     3001 cgccaagcaa caggaccgct ttctcgccat gcgatttcag cacgtcgacg gcacctctga
     3061 cgtcggtgtc gcctggttgg tgcatgccag gatcccactg actgggtggg ccaagtttct
     3121 gcgtcacccg gtacatgtgc ccttcgaccg tcctgatcga tatggtcagt ttctcggcca
     3181 cctcgcgggt ggtatatccc tgctcgacga gcctgacgat ctcccgctcc cgtgtagtta
     3241 gtggcacgtc tggcgcttcc gcatcatcgg attccgcctc actccacgac aggtcggccg
     3301 gcaagacgag cgtcgcgatg cgtccgaagc tcgcagcggc gatcgcggcg tccacagtcg
     3361 gcgcgagctt ggcggccgac gggggtcgat gcaccgtacc gtgaagccag tttcccaacg
     3421 attcgatgtc ggattgcaat ggcgcatcga gcttttggtg gtaggtggcg tggtcgccga
     3481 caacgttgac catcgggctg cacgcccggc gggcgttgtg caggttcgcc agcccgttcg
     3541 ccatccccgg tcccaggtgc agcagggtgg cggcgggctt accggcgatc cgggcgtatc
     3601 cgtcggcggc gccggtagcc acaccttcga acgtgcacag caccgcccgc atctgcggcg
     3661 cggaatccaa cgcggcgacg aaatgcatct ccgaggtgcc ggggttggcg aaacatacgt
     3721 cgactccccc ggcaatcaag cggtcgatca caacctctgc gcccgtagtc acccacccat
     3781 attgacatcc tccactgctt gaaagcggtg gattcccttg gccgttaggc ggcgttgggt
     3841 ggtttgcggt tcatcgcaga cgccgccggc ccaggggccg gacccgtgag ggggtcggtc
     3901 ttgctcttgc tccccgcctg gctgacggga gtgatcccgc ccggcttttc accggatgtc
     3961 ccagccagtt gttcggttcg cgccgtccac cgcgtgagag cctcctcgcg gatgttgacg
     4021 gcggcgttgg tgtcggcgtt gccggtccaa gcacacgaga ggcaacggaa caccgcttgg
     4081 ctttcacggt tccccttgca cgtgtgcccg cactgatggc aacgctgact ggtgtaggcg
     4141 gcgggcacct cgatgaaggg cacctccgcc agattggctt tgtactcgat gaacttggca
     4201 agtttgccga ccgcggcgag ccgcagtgcc cggttgagtc ctcgcttctg cgcggcccca
     4261 gcgcctttgc cccgccgggt catgcggtcc agggacagtt tctcgatccc gatgaaatca
     4321 tgcccgcgca ccaggcgggt gctgagcttg tgcgcgaaat cttcacgcac gcgcgcctgc
     4381 tgggccgaca ggtccgcggc ggcggccttg tgcgcttggt agcgtttcga ctcccaaaac
     4441 gggcgtccct gccgggcggc gatcagcttg gacttggcca ttcgccgctg atgaaacttc
     4501 cgctgtcttt ccagggcggc ggtgtcgggg gcgtcataga acccgc
//
LOCUS       DASRJA010000545         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4035624,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000545 DASRJA010000000
VERSION     DASRJA010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4035624"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VFC01_24125"
     CDS             <1..931
                     /locus_tag="VFC01_24125"
                     /inference="COORDINATES: protein motif:HMM:NF013119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HZN82738.1"
                     /translation="TAFHVSVDARLDTTTGISASDRAATIRALAEPHSRPEDFTRPGH
                     VFPLAARDGGVLERAGHTEAAIDLVKLAGLEPVAVICEIADGDGEMARMPALHAFAEH
                     HQIPLVTIAELAAYRRRTERLIDRVGQARLPLPQGEFTAVGYHDRTNGCEHLALVHGD
                     PTATDCVPVHVHSECLSGDVFGSLVCDCGTQLQRAFELIVDNGAGVVIYLRDGHNHSL
                     QCTPAGQWLDHIESQIVADLGINPDTVALLTDPVVAPIGGKAAVHPACSETFCGDVLS
                     RDLPRYESPADRQRALLHTVLTTGRRRPRKVQL"
     gene            928..2280
                     /locus_tag="VFC01_24130"
     CDS             928..2280
                     /locus_tag="VFC01_24130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82739.1"
                     /translation="MTTLDESKPAAAVPVSEPSKRSGAVYYPTDRDAKRFAHIGKLVD
                     RAAGTGSRPYYDSVVIAGAGLTATVMAARLARSEQFHGKVVLAGPRSEESRRLKDGAT
                     LRGHGADYMCYALGVPQYAFVDALYGDIVDGRGVGTRLLVNMSKKGRSGAFEFGRTFP
                     VMGGYPGTSRPLFYGVRNSRVQAAMHEFMDPAGVIEVPEAVSSLAEAQALAPGKRPLI
                     VNASHNTALLRGQGPKVDWATVAVQAPLTVRPGGFRNITSNAALFTGVRRAGRIVVGL
                     FNPYGDPLSPRATFYGIMVGEVSQRAGFDKQPEIDIMTDELYGIADALNMDVHDPEET
                     LFSGLIPGSPWKAPESAPGTLELQMISHQGVSATFSDGMTSGAAAAVAAAEAVIRGTD
                     PDRAARRAARQITRDRRIYNIERTKLPFAWDIGIRTVPEAISLYPYTINARTNWCSAG
                     "
     gene            2332..>3374
                     /locus_tag="VFC01_24135"
     CDS             2332..>3374
                     /locus_tag="VFC01_24135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN82740.1"
                     /translation="MTTVEKGNTNSAPRHRNSASSPSRSDLTQSATDPSELVEIFAGQ
                     RTAFDRKPAPTLGQRRATLAKLARLIEQNRLLLVEAANSDFGVRASFETEMSEIVGTI
                     SIIRYMRSHLRSWMAPRRRSVSMWYMPAKNRVEPRPLGVVGVVSPWNFPLHLALIPAA
                     TALAAGNRVMLKVSEFTPRTSAVLKRLIEENFSPDTFYVTDGGGEVASRFTTLPFDHL
                     IFTGSTQVGKIVAKAAAENLTPVTLELGGKSPVIVDKNYSLERAATTVAWAKVFNGGQ
                     VCVAPDYVLVPRGREKEFAVAARQAARKLYPKVAGNPQYTAILNQRHYDRITGYITEA
                     RARDLEVITAENP"
BASE COUNT          642 a         1062 c         1047 g          623 t
ORIGIN      
        1 tacggcgttc catgtcagcg tcgacgcgcg cttggacacc actaccggca tctcggccag
       61 tgatcgggca gcgaccattc gtgcgctggc cgagccgcac tcgcggcctg aggatttcac
      121 gcgtcccggg cacgtgtttc cgctggccgc ccgcgacggt ggtgtgctgg agcgcgccgg
      181 gcacaccgaa gccgctatcg atttggtcaa actcgccggc ctcgagcccg tcgcagtgat
      241 ttgtgagatc gccgatggcg acggagaaat ggctcggatg ccggcgctgc acgcattcgc
      301 cgaacatcac cagatcccct tggtcacgat cgccgagctg gcagcctacc gccggcgcac
      361 cgagcgactg atcgatcggg tagggcaggc gcgactaccg ctgccccaag gggaattcac
      421 ggctgtggga tatcacgata ggaccaacgg ctgtgaacat ctcgcccttg tgcatggcga
      481 cccgacagcc accgattgcg ttccggtgca tgtgcacagc gaatgtctgt ccggcgatgt
      541 cttcggttct ctcgtctgcg attgtggcac tcaactgcaa cgcgcattcg agctcatcgt
      601 ggacaacggg gccggcgtag tcatttatct gcgcgacggc cacaaccaca gcctccaatg
      661 cacgcccgcg ggccagtggc tagaccacat cgaaagccag atcgtcgccg acctcggcat
      721 caacccggac acagtcgcgt tactcacgga ccctgtggtc gccccgatcg gcggtaaggc
      781 cgctgtccac cctgcgtgca gcgaaacctt ctgtggggat gtgctttcca gggatctacc
      841 gcgatacgaa agtcctgccg atcgccagcg ggccttgctc cacacagttt taacaaccgg
      901 ccgacgacgc ccacgaaagg tacaactatg acaacgctcg atgagtcgaa accagctgct
      961 gctgtgccgg tttcagaacc gtcaaaacgc agcggcgcgg tgtactaccc gaccgaccgt
     1021 gacgccaaac ggttcgccca tatcggcaag ctcgtcgacc gcgctgccgg gaccgggtcg
     1081 cggccgtact acgactctgt ggtgatcgcc ggcgcaggcc tgaccgccac cgttatggcg
     1141 gcccggttgg cgcgcagcga acagttccac ggcaaggtcg tgttggccgg gccccgctcc
     1201 gaggagtctc gccggctcaa ggatggagcg acgctgcggg gccatggcgc cgattacatg
     1261 tgctatgcgt tgggggtgcc gcagtacgcc ttcgttgatg cgttgtacgg cgatatcgtc
     1321 gatggccgcg gggtggggac gcggttgctg gtgaatatgt ccaagaaggg ccggagcggg
     1381 gccttcgaat tcggccgcac gttccccgtt atgggagggt acccagggac ttcgcgtcca
     1441 ttgttctatg gtgtgcgcaa cagtcgtgtg caggctgcga tgcatgaatt catggacccc
     1501 gcaggtgtga tcgaagtgcc ggaggcggtc agctcgttag ctgaagctca ggcgctggcg
     1561 ccgggtaagc ggcctttgat cgtcaacgcc agccataaca ccgccctgct gcgtggccaa
     1621 gggcctaagg ttgattgggc cacggtcgcg gttcaagccc cattgacagt gcggcccggt
     1681 gggtttcgga acatcacgtc caatgccgcg ctgttcaccg gtgtacggcg cgctggccgg
     1741 atcgtcgtcg gactgttcaa tccgtatggg gatccgttgt caccacgggc gacgttttac
     1801 gggatcatgg tcggcgaagt gtcccagcgg gccggattcg ataagcagcc cgagatcgac
     1861 atcatgaccg acgaactcta tggcatcgcc gatgcgctga acatggacgt tcatgacccc
     1921 gaagaaacgc tgttctcggg gttgattcca ggatcgccct ggaaagcacc cgaatcagcg
     1981 ccgggcacgt tggaactgca aatgatctcc caccaaggtg tttcggcgac cttctccgac
     2041 gggatgacct cgggtgcagc ggcggcagtg gccgccgccg aggccgtgat caggggtacc
     2101 gatcctgatc gcgcggctcg acgcgcagca cgccaaatca cgcgcgaccg ccgaatctac
     2161 aacatcgaaa gaacgaaact gccgttcgcg tgggacatcg gcattcgcac tgtgcccgag
     2221 gccatttcgc tataccccta caccatcaac gccagaacca actggtgcag cgcgggctga
     2281 ctgccctatg gatttgctta ccaccccttg gaccagaaga taggaacgag tatgaccacc
     2341 gtcgaaaagg gaaacaccaa ttctgctcct cgacaccgta attcagcgtc gagcccctcg
     2401 cgcagcgatc tgacgcaatc ggcgaccgat ccttccgaac tcgtagagat ctttgcgggg
     2461 caacgcaccg cgttcgaccg caaaccggca ccgacattgg gccagcgtcg agccacgctg
     2521 gcaaagctgg cgcggctgat cgagcagaat cggctgcttc tggtcgaggc ggccaactcc
     2581 gatttcggcg tcagggcgtc gttcgagact gaaatgtccg aaattgtggg gaccatcagc
     2641 atcatccgct atatgcgcag ccaccttcgg tcatggatgg cgccgcggcg gcgttcggtc
     2701 tccatgtggt acatgccggc caagaaccgt gtcgagccca gacccctggg tgttgtcggt
     2761 gtggtctcgc cgtggaactt tccgctgcac ctggcactga ttcccgctgc caccgcgctg
     2821 gccgcgggca accgggtgat gctgaaggtc agcgagttca caccgcgtac ctcggcggtg
     2881 ctcaaacggt tgatcgagga gaatttcagc ccggacacct tctatgtgac cgatggcggc
     2941 ggtgaggtcg cttcgcggtt caccacgctg ccttttgacc acctcatctt caccgggtcc
     3001 acccaggtcg gaaagatcgt ggccaaagcc gccgccgaaa acctgacccc ggtaaccctg
     3061 gaactgggcg gcaagagtcc ggtgatcgtc gataagaatt attcactcga gcgcgccgcg
     3121 acgactgtcg cctgggccaa ggttttcaac ggcggccagg tctgtgtcgc gccggactat
     3181 gtgctggtac cgcgcgggcg tgagaaggag ttcgcggtcg ctgcgcgcca ggcagcgcgc
     3241 aagctctacc cgaaagttgc tgggaacccg caatacaccg cgattctcaa tcaacggcat
     3301 tacgatcgga tcaccggcta catcactgaa gcccgcgcac gggatctgga agtgatcacc
     3361 gccgagaatc ctga
//
LOCUS       DASRJA010000546        13928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_24232,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000546 DASRJA010000000
VERSION     DASRJA010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13928
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24232"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFC01_24140"
     CDS             complement(<1..469)
                     /locus_tag="VFC01_24140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HZN82741.1"
                     /translation="MTLLPLRALNRATLGRQLLLQRSPMSAIDAIEHLIGMQAQAPFA
                     PYYGLWSRLERFTGEDLSRLLTARKAVRIVLMRGTIHLVSARDCHRLRPLVQPALDRM
                     LRANGTHGKPLAAIDVSAVVDAARQLLDAETLTPGEVGARLAERWPDTPPGALA"
     gene            complement(466..1206)
                     /locus_tag="VFC01_24145"
     CDS             complement(466..1206)
                     /locus_tag="VFC01_24145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZN82742.1"
                     /translation="MPSISASVSTPDGTCPVTLHTPNGTGPWTAVVMYPDAGGVRPTF
                     QEMAARLAGFGHAVLLPDVYYRSGDWEPFQMETVFGDPEQRKRLMTMVGSVTPEMIAS
                     DATAFFDFLASRPEVKGDAFGVCGYCMGGRTSVIVAGRVPDRVAAAGSFHGGGLVTDD
                     ATSPHLLADQMKATVYVAGAENDASFTPDQAETLDKALTAAGVTHTVEFYPAAHGFAV
                     PDNPPYDKDAAERHWIALQQLFETALPN"
     gene            1249..2049
                     /locus_tag="VFC01_24150"
     CDS             1249..2049
                     /locus_tag="VFC01_24150"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZN82743.1"
                     /translation="MPIATPYEDLLRLVLERGTPKSDRTGTGTRSLFGHQMRYDLSAG
                     FPLLTTKKVHTKSVIYELLWFLRGDSNVRWLQERGVTIWDEWASETGDLGPVYGVQWR
                     SWPTPSGEHIDQISAALELLRRDPDSRRNIVSAWNVGEIPQMALPPCHAFFQFYVADG
                     RLSCQLYQRSADLFLGVPFNIASYALLTHMMAAQAGLKAGEFIWTGGDCHIYDNHLEQ
                     VTEQLSREPRPYPELVLAPRDSIFDYTYDDIAIVNYDPHPAIKAPVAV"
     gene            2046..2531
                     /locus_tag="VFC01_24155"
     CDS             2046..2531
                     /locus_tag="VFC01_24155"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZN82744.1"
                     /translation="MTTVGLIWAQSTSGVIGRGGGIPWRLPEDQARFKDITMRRTVVM
                     GRLTWESLPEKVRPLPGRKNVVLTRQADYMADGAYVVPLLQDALTDDITWVIGGEQIY
                     QLALPIARRCEVTEVEIDLPRDDGDAVAPVLDESWVGTEGEWHTSSTGLRYRFFSYVR
                     P"
     gene            2528..3739
                     /locus_tag="VFC01_24160"
     CDS             2528..3739
                     /locus_tag="VFC01_24160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HZN82745.1"
                     /translation="MTTLTAAQARRIAVAAQGFAEPKPKGPVTRAHLRRLISRIQVLQ
                     LDSVSVAVRAHYAPVFSRLGPYDRDVLDRAAWRHTARSPRLLVEYWAHEAALMAVDDW
                     PLLRWRMREYTHGRWRTEVVKKRQRLADDIVAAVTDLGPLTAGQIEAHVGEDHRGKKG
                     PWWDRSDTKWVTEALFSAGVLTTDTRVGFARHYDLTERVLPPDVVAREVDDDEAVREL
                     TLRAATALGVATEPDIRDYFRLSPKHAKPAIAKLVADGELEAVDVDGWTAPAYLRAGQ
                     IIPRRDRGTALLCPFDPLIFFRPRVERMFQFHYRIEIYVPAPKRQFGYYVWPFMLDGC
                     LVGRVDLKAERTRDALHVVGAFAEPEQDKSRVAKALAAELKTMASWLGLAEVSVGNRG
                     DLARPLSRSLG"
     gene            complement(3684..5120)
                     /locus_tag="VFC01_24165"
     CDS             complement(3684..5120)
                     /locus_tag="VFC01_24165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN82746.1"
                     /translation="MDEQRTLESFGYKQELHRSVATVDLVVYGLVFMVPIAPWAIFGT
                     VYNSASGMVPLVYLIGLVAMVFTALAYSQMAKSFPLAGSVFSYVGRGIHPGVGFFAGW
                     GILLDYLLIPTLLYVFAAESMIGLFPDTPRWLWAILFVVVNTVINLLGIDSLKMANRL
                     FLAIELVFVVIFVVIAVRAINGDSLPNVGWSTTPIWNSEVVSAPLLAAALSIAVLSFL
                     GFDGISTLAEESTGKKNPAGRAMIVSLFIVAFLFVTQTWLASLLAGGRDSFSDDEAGN
                     AFFHLVQAASTTGWMNAFFVVNVLAVGFANAMAAQAATSRLLYSMSRDRQLPSFLSKI
                     SSREVPMAALLVVSAISLVLVLFFVGQIALISSLVNFGALFGFALLHVSVVWYYLVRK
                     KSKNYLLHLVVPTLGFVIIAYVLFNADYLAKIGGIVWLGLGAVVFAINKIRGRGVPQL
                     AEEPAVPGEGVSPATATAAVPDRPGSPR"
     gene            complement(5144..6055)
                     /locus_tag="VFC01_24170"
     CDS             complement(5144..6055)
                     /locus_tag="VFC01_24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HZN82747.1"
                     /translation="MTRTTRLVPFRGHETWVQITAPDDPRDGTLPLFVLHGGPGMAHN
                     YVHNIAELAGETGRTVIHYDQIGCGNSTHLPEAPADFWTPDLFVDEFHTVHTALGIDE
                     YHLLGQSWGGMLSAEIAVRQPAGLASVAICNSPASMELWMAGAAELRAQLPPDTQAAL
                     TRHEEAGTVTDPEYLEATNEFYLRHLCRIDPMPQDLVDTIAQMEAEPTVYHTMNGPNE
                     FHVLGTLRGWSIIDRLHLITAPTLVVAGEFDEATPATWQPYVEHIADVQSHVFPDTSH
                     CSHLEKPEEFRAVVAAFVASHDAVARI"
     gene            6144..6911
                     /locus_tag="VFC01_24175"
     CDS             6144..6911
                     /locus_tag="VFC01_24175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZN82748.1"
                     /translation="MAHNGAPATPGLLQQQLDTSTRVDEITDRLITAIAIGEYLPGAR
                     LPVERDLAASLGAGRMTVRAAFARLLERGLIETRRGRGGGSYVLDQWPESSTEAVGRT
                     LRAHLDELRDRCDALCRIQGTVCRAAAESRSDDDVAVLQARFEAYRTAESGLAAQQAD
                     SEFHLAIMDAAHNDVLKQILLDLKATVSIGAPAHVWGEPTTMRSMELRAMREHEQLVR
                     AIADGRGDDADELARHHGAIDFEIITIAMRRAGVLTD"
     gene            complement(6901..7488)
                     /locus_tag="VFC01_24180"
     CDS             complement(6901..7488)
                     /locus_tag="VFC01_24180"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN82749.1"
                     /translation="MNQSDWRAQKKAATKHAIQEQALRLFVEKGYDATTVEEIAAAAG
                     VSHMTFFRYFPRKEEVVEYDEYDPLLEELIVARPADEPPLVALHRAIRVGLEQILATD
                     REALHIRTRLVLQNPVLRARNLLAQDTSRDVLASALARRAGLSEPDLAATVQASAALG
                     AIAPAAMAWAESDDADFVALVDAAFAALETGCVSR"
     gene            complement(7485..8963)
                     /locus_tag="VFC01_24185"
     CDS             complement(7485..8963)
                     /locus_tag="VFC01_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZN82750.1"
                     /translation="MGNVIVAGAGPTGLALACGLRAAGVDVRVFDKAAGPATTSRALG
                     LQPRGVEVLDRLGALGDLPDRGLPIRSVTINVDGRELASFPVGQPTRLGGPRGLIMSQ
                     ADIEGALRDRLAALGGSVEWGRGVSGLKPGPDSVLVRLDDGSELVAGWVVGADGAHSV
                     VRKAMGVGFPGVPIVERFLLADVHADIDLQRRGATSWLRGDMALAAFPLPGGDVWRVM
                     APAPTEFGDQPAPEAIVDYLGARLAAEAGGSIRSTIWTSTFRIHRRLADTYRRGRILL
                     AGDAAHIHSPLGGQGMNTGIGDAENLAWKLALVVSGRADQGLLDTYGAERRPIAKGVL
                     ETTSSVTEIVVSQRRAARLLRDRIAVPLMNQRWVQELITERASQLKVSYRLGPLGAGL
                     SRFLPGVRVGDRIADRTCTRQDGTSMRLYDTLGPAWALLGSEQLADIASERLGDVVAL
                     RGERDALLVRPDGHLAWRGTDAAGLLAWLDRALGPRVGVLTP"
     gene            complement(9025..9999)
                     /locus_tag="VFC01_24190"
     CDS             complement(9025..9999)
                     /locus_tag="VFC01_24190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82751.1"
                     /translation="MGGSSAIPSSPIQFLREATPCELWLQFDFSQSAGNYSQLSGVLA
                     GFAFLAIMLVLSRQHRRGSESDAVREYEQDNRFVTALACACLGLVTAATLFSLLSGEE
                     GCALISGRALSKEVLAGVAFFFSVYTLLFGAVQLVSASALGTHFRFIVAVLAPPLVVS
                     FIVASLNDLALSLANPPDQPAAPHETLLPGWTDSSTSLWNFAHSVLTWLIPTVLAICL
                     ALWLAGFRWRRASGTPGPVGSALARVVSVALTYLPYASLGLVAYAVWRTAMLGRLSAG
                     AHIGAGQAKVLVLVCTVIVVLQSASLSFARGDDRSPDFQANSKVSGTD"
     gene            10094..10846
                     /gene="thyX"
                     /locus_tag="VFC01_24195"
     CDS             10094..10846
                     /gene="thyX"
                     /locus_tag="VFC01_24195"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HZN82752.1"
                     /translation="MAETAPLRVQLIAKTDFLAPPDVAWSTDADGGPALVEFAGRACY
                     QSWSKPNPRTATNAAYLRHIIDVGHFSVLEHASVSFYVTGISRSCTHELIRHRHFSYS
                     QLSQRFVPEHDAEVVVPPGIEDDPELQEIFKAAADASRATYTELLTRLEAKFADQPNA
                     VLRRKQARQAARAVLPNATETRIVVTGNYRAWRHFIAMRASEHADVEIRRLAIECLRQ
                     LIDLAPAVFSDFEISTLADGSEVATSPLATEA"
     gene            10906..11808
                     /gene="dapA"
                     /locus_tag="VFC01_24200"
     CDS             10906..11808
                     /gene="dapA"
                     /locus_tag="VFC01_24200"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZN82753.1"
                     /translation="MSTSGFDVTARLGTVLTAMVTPFKPDGSLDTDAAARLATHLVDS
                     GCDGLVLSGTTGESPTTTDDEKIALLRAVLEAVGDRARVVAGAGTYDTAHSVHLAKAC
                     AAEGAHGLLVVTPYYSRPSQAGLVAHFTAVADATDVPVILYDIPSRSVVPIEWDTIRT
                     LAGHPNIVAVKDAKADLHGGGQIIAETGLAYYSGDDALNLPWLAMGAVGFVSVWGHLA
                     AGQLRDMLSAFTSGDIATARKINVTLGPLSSAQTRLGGVTLAKEGLRLQGIEVGNPRL
                     PQVPATPEQIEALAADMRAAAVLR"
     gene            11808..13484
                     /locus_tag="VFC01_24205"
     CDS             11808..13484
                     /locus_tag="VFC01_24205"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HZN82754.1"
                     /translation="MNEELKPPGPLAPGGLRVTALGGVSEIGRNMTVFEHLGRLLIVD
                     CGVLFPTHDEPGVDLILPDLRHVEDRLDDVEALVLTHAHEDHIGAIPFLLKLRPDIPL
                     VGSKFTLALVAEKCREHRIKPVFIEVAEGQSSKHGVFECEYFHVNHSIPGCLAIAIHT
                     GAGTVLHTGDIKLDQLPLDDQPTDLPGMSRLGDSGVDLFLCDSTNAEIPGVGPSESEI
                     GPNMHRLIRGAEGRVIVACFASNVARVQQIIDASVALGRKVSFVGRSMVRNMGIAKEL
                     GYLNVATDDVVDIAAAEMMAPERVTLVTTGTQGEPMAALSRMSRGEHRSITLTSGDLI
                     IMSSSQIPGNEEAIFGVLDALAKIGVRVVTNAQVRIHVSGHAYAGELLFLYNAVRPRN
                     VMPVHGTWRMLRANAALAARTGVSPDAIMLAENGVSVDLVAGRASIAGAVTTGKMFVD
                     GLITGDVGDATLGERLILSSGFIAVTVVVRRGTGKPAAPAHLYSRGFSEDPKALEAVA
                     QKVEDELEFLASENITEVTRIAQAVRRVVGKWVGDTYRRQPMIVPTVIEI"
     gene            complement(13485..>13928)
                     /locus_tag="VFC01_24210"
     CDS             complement(13485..>13928)
                     /locus_tag="VFC01_24210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82755.1"
                     /translation="SPRHSGRLRAERADRIMALTYLRHAAPAVLLAAVATLGGSAYGN
                     PAVAGAQPQQWDEAGFNECAEGYEAQREDDYVSWYYGVRQCCISYGGVWRDQQPGQAP
                     RCDPPPPMVHIPPGGVIETFSPAPPPPPVTRVPLGDVTATFAPAP"
BASE COUNT         2452 a         4554 c         4649 g         2273 t
ORIGIN      
        1 cggcaagcgc acccggcggc gtgtccggcc atcgctcggc gagccgggcc ccgacttcgc
       61 ctggcgtcag cgtctcggcg tcgagaagtt gtctggccgc gtcgacgacc gctgacacat
      121 cgatggccgc cagcggcttg ccgtgcgtgc cgttggcccg cagcatccga tccagtgccg
      181 gctggaccag cgggcgcagc cggtggcagt ccctggccga cacgaggtgg atggtgccgc
      241 gcatcagcac gatccgaacg gccttgcgtg ccgtcagcag ccgcgacagg tcctcacccg
      301 tgaagcgctc gagccggctc cacaacccgt agtacggcgc gaacggggcc tgcgcctgca
      361 tgccgatgag gtgttcgatc gcgtcgatgg cgctcatcgg cgagcgctgc aagagcagct
      421 ggcggccgag tgtggcccgg ttcagcgccc gcagcgggag cagcgtcaat tgggcagggc
      481 ggtctcgaac agctgctgca gcgcgatcca gtgccgctcg gcggcgtcct tgtcgtacgg
      541 cgggttgtcc ggcaccgcga atccatgcgc ggccggatag aactcgaccg tgtgggtgac
      601 gccggccgcg gtgagcgcct tgtcgagcgt ctcggcctgg tccggagtga acgacgcgtc
      661 gttctcggcg cccgcgacgt agaccgtcgc cttcatctgg tcggcgagca ggtgcgggct
      721 ggtggcgtcg tcggtgacca ggccgccgcc gtggaacgaa cccgccgcgg ccacccggtc
      781 gggcacccgg ccggcgacga tcaccgacgt ccgcccgccc atgcagtaac cgcagacacc
      841 gaacgcgtcg cccttgactt ccggccgcga ggccaggaag tcgaagaacg cggtcgcgtc
      901 ggacgcgatc atctcggggg tcacgctgcc gaccatcgtc atcaagcgct tgcgctgttc
      961 ggggtcgccg aacacggtct ccatctggaa cggctcccag tcgccgctgc ggtagtacac
     1021 gtcgggcagc agcacggcat gcccgaagcc ggccagcctg gccgccatct cctggaacgt
     1081 cggccggacg ccgccggcgt cgggatacat cacgacggcc gtccaggggc cggtgccgtt
     1141 gggggtgtgc agggtcacgg ggcaggtgcc gtcaggggtg ctgacggacg cggagatact
     1201 cggcatggct accgttctac tctcgccgcc ccaagtaagc tgaccggcgt gcccatcgcc
     1261 acgccctacg aggacttact gcgcctggtg ctcgagcgtg gcacaccgaa atcggaccgc
     1321 accggcaccg gcacccgcag cctgttcggc catcagatgc gctacgacct gtcggccggt
     1381 ttcccgctgc tcaccaccaa gaaggtgcac accaagtcgg tgatttacga gctgctgtgg
     1441 ttcctgcgcg gcgactcgaa tgtgcggtgg ctgcaggagc gtggcgtcac gatctgggac
     1501 gaatgggctt cggagacagg tgatctcggc ccggtatacg gggtgcagtg gcggtcgtgg
     1561 ccgacgccat ccggtgagca catcgaccag atcagcgccg cgctggagct gttgaggcgg
     1621 gatccggact cgcgacgcaa catcgtctcg gcctggaacg tcggcgagat cccgcagatg
     1681 gcgttgccgc cctgccacgc gttcttccag ttctatgtcg ccgacgggcg gctgtcctgc
     1741 cagctctacc agcgcagcgc cgacctgttc ctcggcgtgc cgttcaatat cgccagctac
     1801 gcattgctca cccacatgat ggccgcgcag gccggcctca aggcagggga gttcatctgg
     1861 accggcggcg actgccacat ttacgacaac cacctcgagc aggtcaccga gcagttgtcg
     1921 cgtgagccgc ggccgtaccc ggaacttgtt ctggcgccgc gtgattcgat cttcgattac
     1981 acctacgacg acatcgcgat cgtcaactac gacccgcacc cggcgatcaa ggcgcctgtc
     2041 gcggtatgac gacggtcggg ctcatttggg cccaatcgac ctccggtgtg atcggtcgcg
     2101 gtggcggcat tccatggcga ctgccggagg accaagccag gttcaaggac atcaccatgc
     2161 gccgcaccgt ggtgatgggg cggttgacct gggaatcgct gccggaaaag gtccgcccgc
     2221 tgccgggccg caaaaatgtc gtactcaccc ggcaagctga ctacatggct gacggcgcat
     2281 atgtagttcc cctgcttcaa gatgccctga ccgacgacat cacctgggtg atcggcggcg
     2341 agcagatcta tcagctcgcg ctgccgatcg cgaggcgatg tgaggtcacc gaggtcgaga
     2401 tcgacctgcc ccgcgacgac ggcgacgcgg tggcgcccgt gctggacgaa tcgtgggtag
     2461 gcactgaggg ggagtggcac accagctcca ccggtttgcg ctaccggttc ttcagctacg
     2521 tccggccgtg accaccctga ctgccgccca ggcgcgccgc atcgccgtgg ccgcgcaggg
     2581 cttcgccgag ccgaagccga agggtccggt cacccgcgcg catctgcgcc ggctgatctc
     2641 gcggattcag gtgctgcagc tggattcggt ctcggtggcg gtgcgcgccc actacgcgcc
     2701 ggtgttcagc cggcttgggc cctacgaccg tgacgtgctc gatcgtgcgg cgtggcgcca
     2761 caccgcccgc tcaccgcgac tgctggtcga gtactgggcg cacgaggccg cgctgatggc
     2821 cgtcgacgac tggccgctgc tgcgttggcg catgcgcgaa tacacacacg ggcggtggcg
     2881 caccgaagtc gtcaagaagc gtcaacggtt ggctgacgac atcgtcgctg ccgtgaccga
     2941 tctcggtccg ttgacggcag gccagatcga ggcgcacgtg ggcgaagacc accgcggaaa
     3001 gaagggtccc tggtgggacc gaagcgacac caaatgggtg accgaggcgt tgttctctgc
     3061 gggtgtgctg acgacggaca ctcgcgtcgg ctttgcccgc cactacgacc tcaccgaaag
     3121 ggtgttgccg cccgacgtgg tggctcgcga ggtcgatgac gacgaggcgg tccgtgaact
     3181 caccttgcgg gcggcgacgg cgctgggcgt ggcaacggaa cccgatatcc gcgactactt
     3241 ccggctgtcg ccgaaacacg cgaagcccgc gatcgcaaag ctggtcgctg acggggaact
     3301 ggaagctgtc gatgtcgacg gctggacggc tcctgcgtat ctgcgggccg gccagatcat
     3361 tccgcgacgt gatcgcggca ccgcgctgtt gtgcccgttc gatccgttga tcttcttccg
     3421 gccacgggtc gagcggatgt tccaattcca ttaccgcatc gaaatttacg tacccgcgcc
     3481 gaaacgccaa ttcggttatt acgtttggcc tttcatgctc gacggatgtc tcgtcggccg
     3541 tgttgacctg aaggccgaac gcacacgtga tgcgctgcac gtcgtcggcg cgttcgccga
     3601 acccgaacaa gacaagtcgc gggtggccaa agcgcttgcc gccgagttga agacgatggc
     3661 gtcctggttg ggtctggccg aggttagcgt ggggaaccgg ggcgatctgg cacggccgct
     3721 gtcgcggtcg ctgggctgac accttcaccc ggcactgccg gctcctcggc cagttgcggc
     3781 acgccccggc cacgaatctt gttgatcgcg aacaccaccg cgccgaggcc cagccacacg
     3841 atgccgccga tcttggccag gtagtcggcg ttgaacaaca cataggcgat aatcacaaag
     3901 cccagcgtcg gcacgaccag gtgcagcagg tagttcttgg acttcttgcg caccaggtag
     3961 taccaaacca ccgaaacgtg caacagcgcg aagccgaaca gcgcgccaaa gttcaccagt
     4021 gacgagatca gcgcgatctg gccgacgaag aacagcacga gcacgaggct gatcgcgctg
     4081 accacaagga gcgcggccat cggcacctca cggctgctga tcttggacag gaagctcggc
     4141 aactgccggt cgcggctcat cgaatacaac agccggctcg tcgcggcctg ggcggccatc
     4201 gcgttggcga aaccgaccgc cagcacgttg acgacaaaga atgcgttcat ccagcccgtg
     4261 gtggacgccg cctgaaccag atggaagaac gcattgcctg cttcgtcatc actgaacgaa
     4321 tcacgcccgc ccgcaagcag actggccagc caggtctggg tgacgaacag gaacgccacg
     4381 atgaacagtg agacgatcat cgcccgcccc gcgggattct tcttgccggt ggattcctcg
     4441 gccagggtcg agatgccgtc gaagcccaga aagctcagca ccgcgatcga caatgcggcc
     4501 gcgagcagtg gtgcgctgac aacctctgag ttccagatcg gcgttgtgct ccaaccgacg
     4561 ttgggcagcg agtcgccgtt gatcgcgcgc accgcgatga ccacgaagat caccacgaag
     4621 accaactcga tggccaggaa cagccggttg gccatcttca gcgaatcgat tccgagcagg
     4681 ttgatcacgg tgttgacgac gacgaacagg atcgcccaca gccaccgcgg cgtgtcggga
     4741 aacagaccga tcatggactc ggcggcgaac acatacagca gcgtcgggat caacaggtaa
     4801 tccagcagga tgccccaacc ggcgaaaaag cctacgcccg ggtggattcc gcggccgacg
     4861 taggagaaga ccgatcccgc caacgggaac gacttggcca tctgtgaata ggccagcgcg
     4921 gtgaacacca tggccaccag gccgatgagg tacaccagcg ggaccatgcc cgaggcgctg
     4981 ttgtacaccg tgccgaagat cgcccacggt gcgatcggaa ccatgaagac caggccgtag
     5041 accacgaggt cgacggtcgc gacggagcga tgcagttcct gcttgtaacc gaacgattcg
     5101 agcgtgcgct gttcgtccat gagcgtcctg tcttgttgag cggttagatg cgggctacgg
     5161 cgtcgtggct ggcgacgaat gccgcgacga cggcgcggaa ctcctcgggc ttctccaggt
     5221 gcgagcaatg gctggtatcc gggaacacgt ggctttgtac gtcggcaatg tgctcgacgt
     5281 acggctgcca tgtcgccggc gtcgcttcgt cgaactcgcc ggcgacgacg agcgtcggcg
     5341 ctgttatcag gtgcagccgg tcgatgatgc tccagccgcg cagtgtgccc aagacgtgaa
     5401 attcgttggg gccgttcatg gtgtgataga ccgtcggttc cgcctccatt tgggcgatcg
     5461 tatcgacgag gtcctgcggc atcgggtcga tacgacagag gtggcgcagg tagaactcgt
     5521 tggtcgcctc caggtattcc gggtcggtga cggtgcccgc ctcctcgtga cgggtcagcg
     5581 cggcctgggt gtccggcggc agctgcgcgc gtagttcggc ggctccggcc atccagagct
     5641 ccatcgaggc aggggagttg cagatcgcca ctgacgccag cccggcgggt tgccggaccg
     5701 cgatctccgc gctcaacatg ccgccccacg actggccgag caggtggtac tcgtcgatgc
     5761 ccagcgcagt gtggaccgtg tggaactcgt cgacgaacag gtccggcgtc cagaagtcgg
     5821 cgggagcctc gggaaggtgc gtgctgttgc cgcacccgat ctggtcgtag tggatgaccg
     5881 tccggccggt ttcgcccgcc agctcggcga tgttgtgcac gtagttgtgc gccattcccg
     5941 ggccgccatg caggacaaac agggggaggg tgccgtcgcg cggatcatca ggtgcggtga
     6001 tctgcaccca ggtttcgtgg ccgcggaacg gcaccagccg agtcgtacga gtcatggcga
     6061 acagaatgaa cccgtaaagg tctcatgaca agaccattga ccgggattta acatttgtgc
     6121 agacgacgga ggggaatcga ccgatggcgc ataacggcgc acccgcaacg ccggggctgc
     6181 tccagcagca gctcgataca tccacgcggg tcgacgagat caccgaccgg ttgatcaccg
     6241 cgatcgcgat cggcgaatac ctgcccggcg cacggctgcc ggtcgaacgt gacctggcag
     6301 cctcgctggg cgcggggcgc atgaccgtgc gggccgcgtt cgcgcggctg ctcgagcggg
     6361 ggctgatcga gacccgacgc gggcgcggcg gcggatcgta tgtgcttgac caatggccgg
     6421 agtcgtccac cgaagcggtg ggccgcacgc tgcgggcgca cctcgacgaa cttcgggacc
     6481 gctgcgatgc gctgtgccgg atacagggca ccgtctgccg ggctgccgcg gagtcccgct
     6541 ccgacgacga tgtggccgtg ttgcaggcca gattcgaggc ttaccggacc gcggagagcg
     6601 gcctggccgc ccagcaagcc gatagcgagt ttcatctggc gatcatggac gctgctcaca
     6661 acgatgtcct caagcagata ttgctcgact tgaaggcgac tgtgagtatc ggtgctcccg
     6721 cccacgtgtg gggcgagcca acgaccatgc gcagcatgga attacgggca atgcgtgaac
     6781 acgaacaact ggtgcgcgcg atcgccgacg gccgcgggga cgacgccgat gagttggcac
     6841 gtcaccacgg cgccatcgac ttcgagatca tcaccatcgc gatgcgccgc gctggcgtgc
     6901 tcaccgactg acgcagccgg tctccaacgc ggcgaacgcg gcatcgacaa gcgcgacgaa
     6961 gtccgcgtcg tcactttccg cccaggccat cgccgcgggt gcgatcgcgc cgagggcggc
     7021 ggacgcctga accgttgccg caaggtcggg ctcagacaac ccggcgcgac gagccagcgc
     7081 gctggccagc acatcgcgag aagtgtcctg cgcgagcagg tttcgcgctc gcagcacggg
     7141 attctgtagc acgagccggg tccggatgtg cagagcctcg cggtcagtcg cgaggatctg
     7201 ctcgagtccc acgcggatgg ccctgtgcaa cgcgaccaga gggggctcat ccgccggtcg
     7261 cgcgacgatc aactcctcga gcagcgggtc gtactcgtcg tactcgacga cctcctcctt
     7321 gcggggaaag tagcggaaga acgtcatgtg cgaaacgccc gctgctgcgg cgatctcctc
     7381 gacagtcgtt gcgtcatatc ccttttcaac gaagagccgc aaggcctgtt cctggatcgc
     7441 gtgcttggtc gccgccttct tctgtgcgcg ccaatcactc tggttcacgg cgtcagcact
     7501 ccgacgcgcg ggccaagcgc acggtccagc caggccagca atccggcggc gtcggtgccg
     7561 cgccacgcca ggtgtccgtc gggtcgcacc agcagcgcat cgcgttcgcc gcgcagggcc
     7621 acgacgtccc ccaggcgttc agaggcgatg tccgccaatt gctccgaccc aagcaacgcc
     7681 cacgccggac ccagcgtgtc gtacagccgc atggaagtgc cgtcctgacg cgtgcaggtc
     7741 cggtcagcga tgcgatcgcc tacgcgcaca cccggaagaa accgcgagag ccccgccccg
     7801 agcgggccaa gccggtacga aaccttcagc tgcgacgccc tttccgtgat gagctcctgc
     7861 acccagcgct ggttcatcag cggcaccgcg atgcggtcgc gcagcaggcg ggccgcgcgc
     7921 cgttggctca cgacgatctc ggtgacgctg cttgtcgtct cgaggacgcc cttggcgatc
     7981 gggcgtcgtt cggccccgta cgtgtcaagc aggccttggt ccgcgcggcc ggacaccacc
     8041 agggccagct tccatgccaa gttctcggcg tcgccgatgc cggtgttcat gccctgcccg
     8101 cccaacgggc tgtggatgtg cgcggcgtcg ccggccagca ggatgcgtcc gcgccggtac
     8161 gtgtccgcca gtcggcgatg aatccggaag gtcgacgtcc agatcgtcga gcgaatactg
     8221 ccgcctgcct ctgcggccaa tctcgcaccg aggtagtcaa cgatcgcctc gggtgcgggt
     8281 tgatcgccga attccgttgg tgctggggcc atcacgcgcc atacgtcccc tccgggcagc
     8341 gggaaggcgg ctagcgccat atcgccgcgc aaccacgacg tcgcgccacg gcgttgcaga
     8401 tcgatgtccg cgtgcacgtc ggcgagtagg aaccgctcga cgatcggcac gccgggaaat
     8461 ccgacgccca tggccttgcg aacaacgctg tgtgcgccat cggcgccaac gacccatccg
     8521 gcgacgagct ccgaaccgtc gtcgaggcgc acgagcacac tgtccggacc cggcttgagt
     8581 cccgaaacgc ctcggcccca ctcgacgctt ccaccgaggg cggccagccg gtcgcgcagc
     8641 gcaccctcga tatcggcctg cgacatgatc agtccacgag gaccgccgag gcgggtcggc
     8701 tgcccgaccg gaaagctcgc aagctcgcgg ccatcaacgt tgatggtgac gctgcggatc
     8761 ggcaggccgc ggtcgggcag atcaccgagt gcgccgaggc ggtcgagcac ctcgacgccg
     8821 cgcggctgaa gtcccagcgc ccgagacgtc gtcgccgggc ccgccgcctt gtcgaacacc
     8881 cggacgtcga cgccggccgc gcgaagcccg caggccagcg cgaggcccgt cggcccggct
     8941 cccgccacga tgacatttcc catgccgcct cctgtgatgt tagtcactaa catgttaggt
     9001 gactaacagg ccgtagcggt aggcctaatc cgtcccgctc accttggaat tcgcttggaa
     9061 atcgggtgac cgatcgtcgc cgcgggcgaa ggacaggctc gcgctttgca gcacaacgat
     9121 gaccgtgcaa accagcacaa gcacctttgc ctggccagcg ccgatatgcg ccccggccga
     9181 gagccgccct agcatcgctg tccgccaaac ggcatacgcg accaacccga ggctggcgta
     9241 ggggaggtac gtcaacgcga ccgagacgac tcgtgcaagc gcagagccga caggccccgg
     9301 ggtaccgcta gcccgccgcc accggaatcc ggccagccac agcgccaggc atatcgccag
     9361 caccgtcggt atcaaccacg tcaacacgct gtgggcaaag ttccacagcg atgtgctgct
     9421 gtcagtccag ccgggtagaa gcgtttcgtg cggtgcagcc ggttgatccg gggggttcgc
     9481 caaagacagc gcgaggtcat tgaggctggc gacgatgaat gaaaccacga gcggcggcgc
     9541 gagcaccgcg acgatgaatc tgaaatgcgt gcccaatgcg gacgccgaaa cgagttgcac
     9601 cgcaccgaaa agcaatgtgt agaccgagaa gaagaatgcc acaccggcga gcacttcctt
     9661 tgacagcgcc cggccgctga tcagcgcaca accctcttct cccgacagca acgagaacag
     9721 cgtcgcggcc gtcaccaggc cgagacaggc acatgccaag gcggtgacaa agcggttgtc
     9781 ctgttcgtac tcgcggaccg catcgctttc gctgccccgg cggtgctggc gactcagaac
     9841 gagcatgatg gcgaggaagg cgaacccggc caatacgccg gatagctggg agtaattccc
     9901 ggccgactgc gagaagtcga actgcagcca caattcgcac ggcgtcgcct cgcgcaagaa
     9961 ctggatggga gacgacggaa ttgcgctcga gccgcccatg aatctccagc tgatcgttgg
    10021 agtacctcga ccgcgccaac gtagcaggtg aaggggtgcg tcggtccata tcagcgcccg
    10081 atactgtgac ctcgtggccg agaccgcgcc cttgcgcgtg caactgatcg ccaagaccga
    10141 cttcttggcg ccgccggacg tggcgtggag caccgacgcc gacggcggtc cggcgctggt
    10201 ggagttcgcc ggccgggcgt gctatcagag ctggtcgaag cctaatccga ggacggcgac
    10261 gaacgccgcg tacctgcgcc acatcatcga cgtcggccac ttctcggtgc tcgagcacgc
    10321 gtcggtctcg ttctacgtca cgggcatctc gcggtcctgc acacacgagc tgatccggca
    10381 ccggcacttc tcgtattcgc agctgtcgca gaggttcgtc cccgaacacg acgccgaggt
    10441 cgtcgtgccg ccgggtatag aggacgaccc ggagttgcag gagatcttca aggctgccgc
    10501 ggacgccagc agggccacct ataccgagct gctgacgagg ctggaggcca agttcgccga
    10561 ccagcccaac gcggtgctgc ggcgcaagca ggcgcggcag gccgcgcggg cagtgctgcc
    10621 caacgccacc gaaacacgca tcgtggtgac cggaaactac cgggcgtggc ggcatttcat
    10681 cgcgatgcgg gccagcgagc atgccgacgt ggagatccgc cggctggcca tcgagtgcct
    10741 gcgacagctc atcgatctgg cccctgcggt cttctccgac ttcgagatct cgacgctggc
    10801 cgatggcagc gaggtggcga ccagcccgct ggccacggag gcgtaacgac gccgggccat
    10861 atatagccac agaactgtga ggtcgagcag gtaatcttgg gtcccgtgag caccagcgga
    10921 ttcgacgtga ccgcccgatt gggcaccgtg ctgaccgcga tggtgactcc gttcaagccc
    10981 gacgggtcgc tggacaccga tgcagcggcc cggctcgcca cccacctcgt cgactctggc
    11041 tgcgatggcc tggtgctgtc gggcaccacg ggagagtcgc cgaccaccac cgacgacgag
    11101 aagatcgcgc tgctgcgcgc ggtgctcgag gcggttggcg accgtgccag ggtcgtcgcc
    11161 ggtgcgggca cctacgacac cgcacacagc gtccatctgg cgaaggcgtg tgcggccgag
    11221 ggcgcgcatg gcctgctggt ggtgacgccg tattactcgc gtccgtcaca ggccgggctg
    11281 gtcgcgcact tcaccgcagt ggccgacgcc accgacgtgc ccgtgattct ttacgacatt
    11341 ccgtcgcggt cggtcgtgcc gatcgaatgg gacaccatcc ggacgttggc ggggcatccg
    11401 aacatcgtgg ccgtcaaaga cgccaaggcc gacctccacg gcggcgggca gatcattgcc
    11461 gagaccgggc tggcgtacta ctcgggcgac gacgcgctta acctgccgtg gctcgcgatg
    11521 ggcgccgtcg gcttcgtcag cgtgtgggga cacctggccg cgggtcagct gcgagatatg
    11581 ttgtctgcgt tcacttctgg cgacatcgcg acagcacgca agatcaatgt cacgctagga
    11641 ccgctcagta gtgcccagac ccgcctcggt ggcgtgacgc tggccaagga ggggctgcgg
    11701 ctgcagggca tcgaggttgg caacccgcgg ctgccgcaag tgccggccac cccggaacag
    11761 atcgaggcgt tggccgccga tatgcgggca gccgcggtgc tgcggtagtg aacgaagaac
    11821 tcaagccgcc ggggccgctg gccccgggag gcttgcgggt taccgcactg ggcggggtca
    11881 gcgaaatcgg ccgcaacatg accgttttcg agcatctcgg ccgcctgttg atcgtcgact
    11941 gcggcgtgtt gttcccgacc cacgacgagc ccggcgtcga tttgatcctg cccgatctgc
    12001 gtcatgtcga ggaccgcctc gatgacgtgg aggcgctcgt cctgacgcac gcccacgagg
    12061 accacatcgg cgcgattccg ttcctgctga agctgcgccc cgacattccc ctcgtcggct
    12121 cgaagttcac gctcgcgctt gtggcggaga agtgtcgcga acaccgcatc aagccagtgt
    12181 tcattgaagt cgcagagggg cagagctcta agcacggcgt gttcgagtgt gaatactttc
    12241 acgtcaacca ctcgattccg ggctgcctgg cgatcgcgat acacacgggt gcgggcactg
    12301 tgttgcatac gggtgatatc aaactcgatc agctgccgct ggacgaccag ccgaccgacc
    12361 tgccgggcat gtcgcggttg ggtgactcgg gggtggactt attcctctgc gactcaacga
    12421 atgccgagat ccccggagtt ggaccgtcgg agagcgagat cgggccgaac atgcaccggc
    12481 tgatccgcgg cgccgagggg cgggtcatcg tggcttgctt cgcctccaat gtcgctcgcg
    12541 tgcaacagat catcgatgcg tcagtggcgc ttggacgcaa ggtgtcgttt gtcggccggt
    12601 cgatggtgcg caacatggga atcgccaagg aactcggcta cctcaacgtt gcaaccgacg
    12661 acgtggttga catcgccgca gcagaaatga tggcgcccga gcgggtcaca ctggtcacca
    12721 ccggaaccca gggtgagccg atggccgcgt tgtcgcggat gtcccgcggt gagcatcgca
    12781 gcatcacgct gacctcgggc gatctcatca tcatgtcgtc atcgcagatc ccgggcaacg
    12841 aggaagcgat cttcggcgtt ttggacgctc tggccaagat cggagttcgc gtcgtcacca
    12901 acgcgcaagt gcggatacat gtttcaggtc acgcctatgc gggcgagctg ttgttcctgt
    12961 acaacgcggt gcgcccgcgc aacgtgatgc cggtccacgg cacgtggcga atgctgcgtg
    13021 cgaatgcggc gttggcggcg cgcaccggtg tgtcgccgga tgcgatcatg ttggcggaga
    13081 acggcgtcag cgtggatctc gtggcgggcc gcgcttcgat cgcgggtgcg gtgacgaccg
    13141 gcaagatgtt cgtcgacggg ctgatcaccg gcgacgtcgg cgacgccaca ctgggcgaac
    13201 ggctcatcct gtcgtcgggt ttcatcgcgg taacggtcgt cgtgcgccgc ggcaccggca
    13261 agcccgccgc acccgcgcat ctgtattcac gaggcttctc ggaggacccc aaggcgctgg
    13321 aggctgtcgc gcagaaggtc gaggacgagt tggaattcct tgcctccgag aacatcaccg
    13381 aggtcacgcg gatcgcccag gcagtccgtc gcgtcgtcgg caagtgggtt ggggatacct
    13441 accgtcgcca gccgatgatc gtgccgacgg ttatcgagat ctagttacgg agcaggcgcg
    13501 aacgtcgcgg tgacgtcgcc gagcgggacg cgggtcaccg gtggtggtgg cggcgccggc
    13561 gaaaacgtct cgatgacccc gcccggcggt atgtgaacca tcggcggggg aggatcgcac
    13621 cgcggtgctt gtccgggttg ttggtctcgc cacacgccgc cgtatgagat acagcattgg
    13681 cgcacgccgt agtaccaact tacgtagtca tcctcgcgtt gggcctcata gccttccgcg
    13741 cactcgttga agccggcttc atcccactgt tgaggttgag cgccggcgac ggcgggattt
    13801 ccgtatgcgc tgccgcccag cgtggccacc gcggcgagta ggacagccgg cgcggcatga
    13861 cgcaggtatg tgagtgccat gatcctgtcc gctcgctcag cccggagccg gcccgaatgt
    13921 cgcggtga
//
LOCUS       DASRJA010000547        14755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3774229,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000547 DASRJA010000000
VERSION     DASRJA010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14755
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3774229"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFC01_24215"
     CDS             complement(<1..191)
                     /locus_tag="VFC01_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HZN82756.1"
                     /translation="MSQAVRNLSDKRDPIVLHDDEIFAAHVGGKLSVALNTPLDTQRA
                     LSVAYTPGVAQVSRAIAAD"
     gene            complement(240..1229)
                     /locus_tag="VFC01_24220"
     CDS             complement(240..1229)
                     /locus_tag="VFC01_24220"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZN82757.1"
                     /translation="MSTTPLKVAVTGAAGQIGYSLLFRLASGSLLGPDRPIELRLLEI
                     EPALKALEGVVMELDDCAFPLLAGVEIGADANKIFDGVNLALLVGARPRGPGMERGDL
                     LEANGAIFTAQGKALNEVAADDVRIGVTGNPANTNALIAMSNAPDIPKERFSALTRLD
                     HNRAISQLAKKTGAAVTDIKKITIWGNHSATQYPDIFHAEVGGKNAAEVVNDQAWIEN
                     DFIPTVAKRGAAIIDARGASSAASAASATTDAARDWLLGSAEGDWVSMAVLSDGSYGV
                     PEGLISSFPVTTKDGNWSIVQGLEIDDFSRARIDKSTAELVDEKKAVTDLGLI"
     gene            1393..2496
                     /gene="corA"
                     /locus_tag="VFC01_24225"
     CDS             1393..2496
                     /gene="corA"
                     /locus_tag="VFC01_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HZN82758.1"
                     /translation="MPSFRGLPPSLRPVVRPKPTHTSAKTIHVPVARAMVDCGIYVDG
                     QRAPGKYTHAAALNKVHELEREGKEAFVWIGLHEPDEHQMQAVADVFGLHELAVEDAV
                     HAHQRPKLERYDKTLFLVLKTVNYVEHESVALAREIVETGEIMVFVGPDFVVTVRHGE
                     HGGLAGLRKRLDASPATLKLGPYAVMHAIADHVVDKYLDITDLIETDIDAMEEDIFSP
                     RKQTNIECIYLLKREVVELRRAVAPLTLALQRMGTDHNDVISIEVRRYLRDVLDHNIQ
                     ASDRIASYDDMLSSLVHAALGKVAMQQNVDMRKISAWVAIAAVPTALAGIYGMNFDHM
                     PELHWVWGYPAVLLLMATVCTLLYRTFRHNHWL"
     gene            complement(2493..3086)
                     /locus_tag="VFC01_24230"
     CDS             complement(2493..3086)
                     /locus_tag="VFC01_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HZN82759.1"
                     /translation="MIDVLAQVRTHLREHFTLAGITGEPDEASVTFLGTERIDVLRFG
                     PDDDGVSHYVSLGCSQHPMFDPTEMVTDALHGPRAEVVVALRGPSPGGLARSIAIVAA
                     APAVEGLILEPDALIDLESPLWDSAPFTAFLLGRSDIDDVPLTAPLPGVTVLSATPIT
                     ATEAAWVRLKGADAMREAWDQDGVDVLDQSRRAARPS"
     gene            complement(3083..4354)
                     /gene="ugpC"
                     /locus_tag="VFC01_24235"
     CDS             complement(3083..4354)
                     /gene="ugpC"
                     /locus_tag="VFC01_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HZN82760.1"
                     /translation="MAEIVLDRVTKSYPNGAAAVKDLSVTIADGEFIILVGPSGCGKS
                     TTLNMIAGLEDISSGELRIGGQRVNEKAPKDRDIAMVFQSYALYPHMTVRQNIAFPLT
                     LAKVKKDEIATKVDEAAKVLGLTELLDRKPGQLSGGQRQRVAMGRAIVRNPKAFLMDE
                     PLSNLDAKLRVQMRSEIARLQNRLGTTTVYVTHDQTEAMTLGDRVVVLLGGVAQQIGT
                     PDELYNRPANLFVAGFIGSPAMNFFPATLTDVGVRLPMGEITLTQEVHDLLARHKRPN
                     NVIVGIRPEHLEDAALIDTYQRIRALVFEVNADVVESLGAEKYVHFKIEGAGAQSAQL
                     EAAAESEVGENEFVARVSTESKVAQGQTLQLAFDTSKIAIFDADTGVNLTIEPAGSQE
                     PTPAPSPAPEPPAEPAASPPPPPSEQTPPAE"
     gene            complement(4359..5183)
                     /locus_tag="VFC01_24240"
     CDS             complement(4359..5183)
                     /locus_tag="VFC01_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZN82761.1"
                     /translation="MGAGRATGWTIINVLVVIYALFPVLWILSLSLKSTATVKDGKLI
                     PAEITFDNYKAIFEGGGFSSALINSIGVGLITTAIAVVIGGMAAYAVARLDFPGKQLL
                     IGVALLIAMFPHISLVTPIFNIERALGLFDTWPGLIIPYITFALPLAIYTLSAFFREI
                     PWDLEKAAKMDGATPGQAFRKVIAPLAAPGIVTAAILVFIFAWNDLLLALSLTATERA
                     ITAPVAIANFTGSSQFEEPTGSIAAGAIVITIPIIIFVLIFQRRIVAGLTSGAVKG"
     gene            complement(5197..6126)
                     /locus_tag="VFC01_24245"
     CDS             complement(5197..6126)
                     /locus_tag="VFC01_24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZN82762.1"
                     /translation="MTTATAEPTAAPPAPRATEDVRSERRLAYLLIAPAVVLMLVVTA
                     YPIGYAVWLSLLRYNMATPDDTAFVGVSNYVTILTDRYWWTAFLVTLAITIISVAIEL
                     VLGMALALVMHRTIFGRGVVRTVVLIPYGIVTVVAAYSWYYAWTPGTGYLANLLPEGS
                     APLTEQIPSLAIVILAEVWKTTPFMSLLLLAGLALVPEDLLRAAQMDGAGAWKRLIKV
                     IIPLIKPAILVALLFRTLDAFRIFDNIYIMTAGSNNTGSVSILGYDNLFKAFNVGLGS
                     AISVLIFLSVALIAFIFIKGFGAAAPGADEGGR"
     gene            complement(6123..7520)
                     /locus_tag="VFC01_24250"
     CDS             complement(6123..7520)
                     /locus_tag="VFC01_24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN82763.1"
                     /translation="MRARRLGAAALAALTTASTVAACGSGGGGLVINYYTPANEAQTF
                     TAVAKRCNEELNGRFTIQQVSLPKGADDQRLQLARRLTGNDKTLDVMALDVVWTAEFA
                     EAGWALPLSDDPTGKAEDDATSNTLPGPLETAKWQDKLYAAPITTNTQLLWYRADLMD
                     EAPKTWDGMLSEATRLDAAGKPSWIAVQGKQYEGLVVWFNTLLESAGGQVLSDDGKTV
                     TLTDTQEHRAATVKALQIIKSVATAPGADPSITQTDETTARLALEQGNAALEVNWPYV
                     LPSLLENAVKGGVSFLPLDKDPALEGSINDAGTFSPTDEQFKTAYDASKKVFGFAHYP
                     GVLPGESAKVTIGGLNLAVAKTTQHKAEAFEAIRCLRNVENQRYTSVEGGLPAVRTSL
                     YEDPTFQAKYPQYAIIRDQLTNAAVRPATPVYQAVSTRISATLAPISDIDPERTADEL
                     AEQVQKAIEGKGLIP"
     gene            complement(7665..8192)
                     /locus_tag="VFC01_24255"
     CDS             complement(7665..8192)
                     /locus_tag="VFC01_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HZN82764.1"
                     /translation="MTSPFQPGQNSGVTPGSPTPGRGGPAALPTPPKGWPIGSYPTYA
                     EAQRAVDYLSDQEFPVQQVTIVGVDLMQVERVTGRLTWPKVLGGGVLTGAWLGLFIGL
                     ILGFFSPSPWGALITGLVAGVFFGLITSAIPYAMARGTRDFSSTMQLVAGRYDVLCDP
                     QNAERGRDLLARLTI"
     gene            8300..8935
                     /locus_tag="VFC01_24260"
     CDS             8300..8935
                     /locus_tag="VFC01_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HZN82765.1"
                     /translation="MTNPGEDAGQTPSSDSSGGASEPSSGGYEAPPIEQSQPQPGPSE
                     QPGYTPPPAYIPPSYEAPSYQQQQSDSPFPPPDYPAPPPYPGPAGYGAPSYPPPQYGT
                     PQYGAPTPGYGTPPPAGYGPPTGYPAPDYSAYGQPAQKTNSLAIASIVASCVGVLCGI
                     GSIVGIVLGIVAMNQIKQTREGGHGLAIAGIAVGSVTLLLSVIFTIAALSS"
     gene            8938..9414
                     /locus_tag="VFC01_24265"
     CDS             8938..9414
                     /locus_tag="VFC01_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HZN82766.1"
                     /translation="MTTSEPERPSGPTEYPSLEDTARQPDPTPTFDYPADAGLPPPVN
                     PPTYAAGYPGYPAAPGYYAGGYNPYRPAKPPGTNGMAIAALAVSAASLVLFCGIPSFI
                     GAILGIIAMRETKRTGQDGYGMALAGTIIGAIPTVLWLLYLFVLIGIYASGWQWAP"
     gene            complement(9500..10120)
                     /locus_tag="VFC01_24270"
     CDS             complement(9500..10120)
                     /locus_tag="VFC01_24270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82767.1"
                     /translation="MGTTAIGRVGAVAVMLGVGAALAATPWAAGVAAASPNELHANAR
                     SDNPRQPDQNRAISTNGVVHVQKGTAQAFSSGQGSTAIADGFNARASANGDGNHAQAD
                     GLSAVAVAGGVSTESSTGVNNRVFVKGDNSSGTAVGGNGNKVDVNGDGNSIFVQGGSN
                     DVTVKGDNNNPFNVLGDANTVKICGNGNQQTITGNGQKTTIGKCGR"
     gene            complement(10510..11466)
                     /locus_tag="VFC01_24275"
     CDS             complement(10510..11466)
                     /locus_tag="VFC01_24275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZN82768.1"
                     /translation="METAYRPRRTCLSVPGSNPKMIEKAKNLPADEVFLDLEDAVAPD
                     AKAEARTQVAVALAEPGWAEQLRGVRVNDWTTPWTYADVIEVVSTAGASLDIIVLPKV
                     TDVAHVHALDLLLSQLETNHGLPLGRIGIEVQIENVQGLTNIDAIAAGPRVQALILGP
                     ADMAASLNMRTLEVGEQPEGYDVGDAHHHVLMRILIAARTHGVLAIDGPYLKIRDVEG
                     FRRVAGRSAALGYDGKWVLHPEQIAAGNEVFSPRQSDYDHAELILEAYEWHTSRVGGA
                     RGAVMLGDEMIDEASRKMALVIAGKGRAAGMERTAEPFRPPA"
     gene            11493..12785
                     /locus_tag="VFC01_24280"
     CDS             11493..12785
                     /locus_tag="VFC01_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZN82769.1"
                     /translation="MAAVNRVYVARLAGMVVLGPDGESIGRVRDVVISIGVSGHQPRV
                     LGLVVELLTRRRIFVPILRVTAIEPSAVTLATGNVSLRRFSKRPNEVLVLGEVIETRV
                     RVDDPELPELAGIDAVVVDLGIEQTRTRDWVVTKVAVRAQRRLGRRGNISIADWNRVQ
                     GLTPSGLAMPDQGVAQLLEQFEGQRPIEVAEAIRELPAKRRYEVLKALDDERLADVLQ
                     ELPEDEQADVLRSLEAERAADVLEAMDPDDAADLLGTMTPADAEQFLRRMDPEDSEDV
                     RRLLSHSPDTAGGLMTSEPVVLAPDTTVAEALARVRDPDLTPAVASLVFVTRPPTATP
                     TGHYLGCVHLQRLLREPPAELVSGIVDKDLPSLSPEDPLAAVTRYFAAYNLVCGPVVD
                     DENHLLGAVSVDDVLDHLLPDDWREREEPELSGAEGVT"
     gene            12782..13333
                     /locus_tag="VFC01_24285"
     CDS             12782..13333
                     /locus_tag="VFC01_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HZN82770.1"
                     /translation="MSDLSARQRLDTPRTTRRRFSLNVDADAVGQFSESIARFLGTGR
                     YLMWQTILVIVWITLNLFAVKLRWDPYPFILLNLAFSTQAAYAAPLILLAQNRQENRD
                     RVALEEDRRRAERTKADTEYLARELAALRLAVGEVVTRDYLRRELEELHEKLTELQQP
                     QTATDGDAASNPDSSERRTKRPG"
     gene            13421..>14755
                     /locus_tag="VFC01_24290"
     CDS             13421..>14755
                     /locus_tag="VFC01_24290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HZN82771.1"
                     /translation="MHIGGRAALEAARRRAGRAVRKPAFGVVIITPLILAGAVGASGP
                     SPLTPVRDSAVTPLAAVELSPGNRSGASVVAVTKTPTPFHIAAATSSAPPPSVIVNSP
                     GTLRIPAIVLSAYRNAERMMAAAAPGCGVSWNLLAGIGRIESLHANGGATDTRGTAVR
                     PIYGPALDGTLPGNEIIVQSRTADRVMYARAMGPMQFLPGTWSRYASDGDGDGKADVQ
                     NVFDASLATARYLCSGGLNLRDAGQVMSAILRYNNSIAYARNVLGWAAAYATGVLPVD
                     LPPIVGSVPPIGDFHLDNYEGLGPGLPMNALGLPATDPLAMMPMMVNGDVASQIPVYP
                     GQALGPLPGPAQLVPQQVAQPEQPAAPPWLPPWMQQPAPQPQRPPECAVFCITSTPAP
                     NAVPPAPGDAQMLMPQAPPAPAEPLTPVPAPAEQPAAVLPPAPGPAPGPAPGP"
BASE COUNT         2547 a         4913 c         4677 g         2618 t
ORIGIN      
        1 tggtccgccg cgatcgcacg gctgacctga gcaacgccgg gcgtataggc gaccgacagt
       61 gcacgctggg tgtcgagcgg cgtgttcagc gccaccgaca gctttccgcc cacatgcgca
      121 gcgaagatct cgtcgtcgtg caggacgatc gggtctcttt tgtcagacag atttctcacc
      181 gcttgggaca cggcgctcag gttactgacg aactggtagg taacctaatc gactttgtct
      241 cagataagtc ccaggtcagt gacggctttt ttctcgtcga ccagctcagc ggtcgacttg
      301 tcgatgcgcg cccgggagaa gtcgtcgatc tccagaccct gcacgatcga ccagttgccg
      361 tccttggtgg tcaccgggaa cgacgagatc aggccctccg gcacgccgta gctgccgtca
      421 gagaggaccg ccatcgacac ccagtcgccc tcggcgctgc ccagcagcca gtcgcgggcg
      481 gcgtcagtgg tcgccgacgc tgccgacgcg gccgacgagg cgccgcgggc atcgatgatc
      541 gccgcgccgc gcttggcgac cgtcgggatg aagtcgttct cgatccaggc ctggtcgttg
      601 accacctcgg cggcgttctt gccgccgacc tcggcgtgga agatgtcggg gtactgggtg
      661 gccgagtggt tgccccagat cgtgatcttc ttgatgtcgg tgacggcagc gccggtcttc
      721 ttggcgagct gcgagatggc ccggttgtgg tcgagacggg taagcgccga gaaccgctcc
      781 ttcgggatat ccggcgcatt gctcatcgcg atcagggcgt tggtgttggc cgggttgccg
      841 gtcacaccga tgcggacgtc gtccgcggcg acctcgttga gcgccttacc ctgcgcggtg
      901 aagatcgcgc cgttggcctc gagcaagtcg ccacgctcca tgcccggccc gcgcggacga
      961 gcgccgacca gcagcgccag gttcacgccg tcgaagatct tgttggcgtc cgcgccgatc
     1021 tcgacacccg ccagcagcgg gaacgcgcag tcatcgagct ccatcacgac accctcgagc
     1081 gccttgagag ccggctcgat ctccagcaga cgcaattcga tcgggcggtc ggggccgagc
     1141 agcgatccgc tcgcgaggcg gaacaacagg ctgtagccga tctggccggc ggcaccggtg
     1201 acggcgacct tgaggggagt tgtgctcacg tcagatgctc cttgggcgtc gattgggtcc
     1261 gggacgaaac tagcgcacgg gcctcggctg tgaatcttcg ggacgtacct gctcatggac
     1321 ggtgcgggta tagccgcgcg cgtagcatta atcgacgctc gatcggacct gcgcagcgac
     1381 gggaggttga cgatgccatc gtttcgggga ttacctccgt cgcttcgacc agtcgtacgg
     1441 cccaagccga cccacacaag tgcaaagacc attcacgttc ccgtggcgag ggccatggtc
     1501 gactgcggca tctacgtcga cggtcaacgg gcgcccggca agtacaccca cgccgccgcg
     1561 ctgaacaaag tccacgaact ggaacgcgaa ggcaaagaag cgttcgtatg gatcggcctg
     1621 cacgaacccg atgagcacca gatgcaggcc gtggccgatg tgttcgggct acacgaactg
     1681 gctgtcgagg atgccgtgca cgcccaccaa cggcccaagc tggagcgcta cgacaagacg
     1741 ctgttcctgg tgctcaagac cgtcaactac gtggagcacg aatcggttgc cctggcccgc
     1801 gagatcgtcg agaccggcga gatcatggtc ttcgtcggcc ccgatttcgt cgtgaccgtt
     1861 cggcacggcg agcacggcgg gctggccggg ctacgcaagc ggctcgacgc ctcccccgcg
     1921 accctcaagc tcggcccgta cgcggtgatg cacgcgatcg ccgaccatgt cgtggacaag
     1981 tacctcgaca tcaccgatct gatcgagacc gatatcgacg cgatggaaga agacatcttc
     2041 tcgccgcgca agcagaccaa catcgagtgc atctatctgc tcaagcgcga ggtcgtcgaa
     2101 ctacgccgag cggtcgcgcc gttgacgctg gcgttgcagc ggatgggcac cgaccacaac
     2161 gatgtgatct ccatcgaggt caggcgctac ctacgcgatg tgctcgacca caacatccag
     2221 gcctccgacc ggatcgccag ctacgacgac atgctcagct cgctggtgca cgccgcgctg
     2281 ggcaaggtgg cgatgcagca gaacgtcgac atgcgcaaga tctcggcgtg ggtggccatc
     2341 gcggccgtgc cgaccgcgct ggcgggcatc tacggaatga acttcgacca catgcccgaa
     2401 ttgcattggg tgtggggcta tccggcggtt ctgctgttga tggccaccgt gtgcacgctc
     2461 ctgtaccgaa cgttccggca caaccactgg ctctaactcg ggcgtgcggc gcgccgtgac
     2521 tggtccagca cgtcgacccc gtcttgatcc cacgcctcac gcatggcgtc ggcgcccttg
     2581 agtcgcaccc acgcggcctc ggtcgcggtg ataggcgtcg ccgacagcac ggtgacgcct
     2641 ggcagcggtg cggtcagcgg gacgtcgtca atgtcgctgc ggcccaacaa gaacgcggtg
     2701 aacggcgccg aatcccacag cggcgactcg agatcgatca gggcatccgg ttccaatatc
     2761 agtccttcga cggccggcgc agcggcgacg atcgctatgg agcgggccag tccgccgggc
     2821 gatggaccac gcaacgcgac gacgacctcg gcccgcggac cgtgcagcgc gtcggtcacc
     2881 atctccgtag ggtcgaacat cggatgttgc gaacaaccaa gcgacacata gtgactgacg
     2941 ccatcgtcgt cggggccgaa ccgcagcacg tcgatgcgtt cggtgcccag gaacgtcacg
     3001 ctggcctcgt cgggttctcc ggtgatgccg gccagggtga agtgctcgcg cagatgcgtg
     3061 cgtacctggg cgaggacgtc gatcactcgg ccggtggcgt ctgctcgctc ggtggcgggg
     3121 gtggcgacgc cgcaggctca gcggggggtt cgggcgccgg tgacggggca ggcgtcggct
     3181 cctgcgagcc cgccggctcg atcgtcaggt tcacgccggt atccgcgtcg aagatcgcga
     3241 tcttcgacgt gtcgaacgcc aactggagtg tctgcccctg cgccaccttg gactcggtgg
     3301 aaacccgtgc cacgaactcg ttttcgccga cctcggattc ggcggccgcc tccaactgag
     3361 ccgactgcgc acccgcgccc tcaatcttga agtgcacgta cttctcggcg cccagcgatt
     3421 ccaccacgtc ggcgttcacc tcgaacacca gcgccctgat ccgttgataa gtgtcgatca
     3481 gtgcggcgtc ctccagatgc tcgggccgga tgccgacgat cacgttgttg ggcctcttgt
     3541 gccgtgcgag cagatcgtgg acttcttggg tcagcgtgat ctcgcccatc ggcagacgca
     3601 ccccaacgtc ggtcagcgtc gccgggaaga agttcattgc cggtgatccg atgaagccgg
     3661 cgacaaacag attggccggc ctgttgtaca gctcgtcggg tgtgccgatc tgctgggcca
     3721 cgccgcccag tagcaccacc acccgatcgc ccagcgtcat tgcctcggtc tggtcgtgcg
     3781 tcacgtagac ggtggtggtg cccaaccggt tctgcaaccg cgcgatctcc gaccgcatct
     3841 gaacccgcag cttggcgtcc agattcgaca gcggctcgtc catgaggaac gccttggggt
     3901 tgcgcacgat tgcgcggccc atagctaccc gctgccgctg accgcccgag agctgccctg
     3961 gcttgcggtc caaaagctcg gtcaggccaa ggactttcgc cgcctcgtca accttggtgg
     4021 caatctcgtc cttcttcacc ttcgccaggg tcagcggaaa cgcaatgttt tggcggacgg
     4081 tcatatgcgg gtagagcgca tacgactgga acaccatcgc gatatcgcgg tccttggggg
     4141 ccttctcgtt gacccgttga ccgccgatac gcagctcgcc cgacgagatg tcctcaaggc
     4201 cggcaatcat gttcagcgtg gtggactttc cgcagccgga cgggccgacc agaatgatga
     4261 actcgccgtc ggcgatggtg acggagagat ccttcaccgc cgccgcgccg ttcgggtaac
     4321 tcttcgtcac ccggtccaac acaatttcgg ccatcgaact acccctttac cgcgccggac
     4381 gtcagaccgg cgacgatccg tcgctggaag atcagaacaa agataatgat cgggatggtg
     4441 atgacgatag cgccagccgc gatcgacccc gtcggttctt cgaattgtga actgccggtg
     4501 aaattggcaa tggccaccgg tgcagtgatt gcccgctcgg tggcggtcag cgacaaggcc
     4561 agcaataggt cgttccacgc gaagatgaac accaggatcg cggcggtgac gatcccgggc
     4621 gcagccagcg gcgcgatcac ttttcggaac gcctgaccgg gagtggcgcc gtccatcttg
     4681 gccgccttct ccaaatccca agggatctcg cggaagaacg ccgaaagggt gtaaatggca
     4741 agcggcaacg cgaaagtgat gtacggaatg atcagccccg gccatgtgtc gaacaaaccg
     4801 agcgcgcgtt cgatgttgaa aatcggtgtc accagcgaga tatgggggaa catcgcgatc
     4861 agcagtgcta cgccgatgag gagttgcttg cccgggaaat ccaaccgggc caccgcgtac
     4921 gccgccatgc cgccgatcac gaccgcgatc gctgtggtga tcaggccgac gccgatggag
     4981 ttgatcagcg cggagctgaa cccgccgcct tcgaagatgg ccttgtagtt gtcgaacgtg
     5041 atctcggccg gaatcagctt gccgtccttg actgttgccg tcgacttcag cgacaggctc
     5101 aggatccaca gcaccgggaa cagcgcgtag atgaccacga ggacgttgat gatcgtccag
     5161 cccgtggcgc gcccggcacc cacccggtcg gccatctcag cgacctccct cgtcggcacc
     5221 cggcgcggcc gcgccgaagc ccttgatgaa gatgaacgcg atcagcgcga ccgacaaaaa
     5281 gatcagcacg ctgatcgccg agcccaagcc aacgttgaac gccttgaaca ggttgtcgta
     5341 acccaggatc gacaccgaac ctgtgttatt cgatcccgcg gtcatgatgt agatgttgtc
     5401 gaagatgcgg aacgcgtcga gggtgcggaa cagcagtgcc accaggatgg ccggtttgat
     5461 cagcgggatg atcaccttga tcagccgctt ccaggctcca gccccgtcca tctgcgccgc
     5521 gcgcagcaga tcctctggca ccaatgcaag tccggcaagc agcagcaacg acatgaacgg
     5581 agtggtcttc cagacttccg cgagaatgac gatggcaagc gacgggatct gttcggtgag
     5641 cggcgctgag ccttcgggaa gcagattggc caagtaaccg gtgccgggcg tccacgcgta
     5701 gtaccagctg taggccgcga ccaccgtcac gatgccgtac ggaatgagga ccacggtgcg
     5761 gacgacgcct ctaccgaaga tcgtccggtg catgaccagc gccagcgcca taccgagcac
     5821 caactcgatc gccaccgaga tgatggtgat tgccagcgtg accaggaacg ccgtccacca
     5881 ataccggtcg gtaaggatgg tgacgtagtt ggacaccccg acgaacgccg tgtcgtccgg
     5941 ggttgccatg ttgtagcgca gcagactgag ccacaccgcg tagccgatcg gataggccgt
     6001 caccaccagc atcaggacga cggcgggcgc gatcaatagg tacgccagcc tgcgctcgga
     6061 acgcacgtcc tcggtggcgc gcggggccgg gggtgcggcg gtcggttcgg cggtggccgt
     6121 ggtcatggga tcagcccttt accctcgatg gccttctgga cctgctcggc gagctcgtct
     6181 gccgtccgct cggggtcgat gtcggagatc ggcgccagcg tcgccgagat tcgggtggac
     6241 accgcctgat agacgggcgt ggccggccgc accgcggcat tggtcagctg gtcgcggatg
     6301 attgcgtact gcgggtactt ggcctggaac gttggatcct cgtacagcga cgtccggacg
     6361 gcaggcagcc cgccctcgac cgaggtgtag cgctggttct cgacgttgcg caggcagcgg
     6421 atggcctcga acgcttccgc cttgtgctga gtggtcttgg cgacagccag gttgaggcca
     6481 ccgatcgtca ctttcgccga ctcacccggc agcacccccg gatagtgcgc gaacccgaac
     6541 accttcttgc tggcgtcgta cgcggtcttg aactgctcgt cggtcggcga gaaggtgccg
     6601 gcgtcgttga tgctgccctc cagggctggg tccttgtcca ggggcaggaa ggacaccccg
     6661 cccttgaccg cgttttccag cagcgaaggc agcacgtatg gccagttgac ctccaacgcg
     6721 gcgttgccct gttccagcgc caaccgtgcc gtggtctcat cggtctgggt aatggacggg
     6781 tcggcgcccg gcgcggtggc aaccgatttg atgatttgaa gcgccttgac cgtcgccgcg
     6841 cgatgttctt gggtgtcggt gagagtgacg gttttgccgt cgtcggagag cacttgcccg
     6901 ccagcacttt ccagcaaagt attgaaccac acgacgaggc cctcatactg tttgccttgc
     6961 acggcaatcc agctcggctt gcccgcggcg tccagccgcg tcgcctctga gagcatgcca
     7021 tcccatgtct tgggcgcctc gtccatcaaa tctgcgcgat accaaagcaa ttgggtgttc
     7081 gtcgtgatgg gcgcggcgta caacttgtcc tgccacttgg cggtttccag cggcccgggc
     7141 agcgtgttgc tggtggcgtc gtcttcggcc tttccggtcg gatcatccga aagcggcaaa
     7201 gcccaacccg cctcggcgaa ctcggcggtc cacaccacgt ccagcgccat cacgtccagc
     7261 gtcttgtcat tgccggtgag ccgccgagcc aattgcaaac gttggtcgtc ggcgcccttc
     7321 ggcaagctca cctgctggat ggtgaagcgg ccgttgagtt cttcgttgca gcgcttggcg
     7381 accgcggtga acgtctgcgc ctcgtttgcc ggggtgtagt aattgatgac gagtccgccg
     7441 ccgcccgaac cacacgccgc cactgttgac gccgtcgtca gtgcggccaa tgccgcagca
     7501 cctagccgcc gagcgcgcac gacacgcctc caagtccgca gggaacctcc cgcgggcaaa
     7561 ccgtattgcc agataggcgt gattcgcaac actttcggcg cgcgcgtcgc acaccgttat
     7621 gcaatcgcga gcgaccgcat actcgctgtt gccgctggtg tcggtcagat cgtcaagcgc
     7681 gccagcagat cccggccgcg ttctgcgttc tgcgggtcgc acagcacgtc gtaccggccc
     7741 gcgacgagct gcatcgtcga actgaaatct cttgtgccgc gcgccatcgc ataagggatc
     7801 gccgaggtga tcaagccaaa gaagacaccc gcgaccaggc ccgtgatcag tgcgccccac
     7861 ggactggggc tgaagaagcc gaggatcagg ccgatgaaca ggccaagcca cgcacccgtc
     7921 agcacgccgc cgccaagcac tttgggccag gtcagcctgc cggtgacccg ttcgacctgc
     7981 atcaggtcaa cgccgacgat ggtcacctgc tgcacgggga actcttgatc ggacaggtag
     8041 tccaccgcgc gctgcgcttc ggcataggtc gggtacgagc cgatcggcca gcccttcggc
     8101 ggagtgggca gagcggccgg gccaccacgc cccggcgtcg ggctacccgg cgtcacgcca
     8161 gagttctgtc cgggctgaaa cgggctggtc atcggtcttc tatctcctct acgcgcgcct
     8221 gatttagaca gctgctctca ctcggaccaa cgctgcgacg gccgcaatgg tgccctgcag
     8281 ttgcgctagg ttgatcagca tgacaaatcc gggcgaggac gcaggccaaa cgccatcatc
     8341 cgactccagc ggcggcgcgt cagaaccgtc ttccgggggt tacgaagcgc cgccgatcga
     8401 gcagtcccaa ccgcagcccg gcccgtccga gcagcccggc tatacgccgc cgcctgccta
     8461 catcccgccc tcgtacgagg cgcccagcta ccagcaacag cagagcgatt cgccctttcc
     8521 gccgcccgac tacccggcgc cgccgcccta ccccggtcct gccggctacg gcgcgccgtc
     8581 gtacccgcca ccgcaatacg gcacaccgca gtacggggcg ccgactcctg gctatgggac
     8641 gccgccgcct gccgggtacg gcccgccgac tggctatccc gcgcccgact actcggctta
     8701 cgggcagccg gcccagaaga cgaactcgct ggcgatcgcc tcgatcgtcg cttcgtgcgt
     8761 cggtgtgctg tgtggcatcg gttcgatcgt cggcatcgtg ctcggcattg tggcaatgaa
     8821 ccagatcaag caaacccgag agggtggcca tgggctcgcg atcgccggca tcgctgtcgg
     8881 ttcggtgacg ctcctgctga gcgtgatctt tacgattgcc gcccttagca gctgacgatg
     8941 accaccagcg agcccgaacg gccgtccggg cccaccgaat acccgtcgct ggaggacact
     9001 gcgcggcagc cggatccgac accgacgttc gactatccgg ccgatgccgg gttgcccccg
     9061 ccggtcaatc cgccgacgta tgccgcgggt tacccgggct atcccgcggc gcccgggtat
     9121 tacgcgggcg gctataaccc gtatcggccg gccaaaccgc ccggcacgaa tggcatggcg
     9181 atcgcggcgt tggctgtttc ggcggcatcg ttggtgctgt tctgcggcat accgtccttc
     9241 atcggcgcga ttctcggcat catcgcgatg cgtgagacca agcgcacggg acaagacggc
     9301 tacgggatgg cgctggccgg caccattatc ggtgccatac ccaccgtgct ttggctgctc
     9361 tacctgttcg tgttgatcgg catctacgcc agcggttggc agtgggcgcc gtagcggacg
     9421 agtccgcacc caactgccac ccactggttg gcatcgcgta gacgaaacca actacgtcgc
     9481 gcggtattac gccgcggtgt cagcgaccgc actttccaat agtcgttttt tgaccgttac
     9541 ccgtgatggt ctgctggttg ccattgccgc agatttttac ggtgttggcg tcgcccagaa
     9601 cattgaacgg gttgttgtta tcgcctttga cggtgacgtc attgctgccg ccctgaacga
     9661 agatcgagtt cccgtcgcca ttgacatcga ccttgttccc gttgcccccg accgcagtgc
     9721 ctgagctgtt atcgcccttg acgaagaccc tgttgttcac gcctgtcgag gactcagttg
     9781 atacgcctcc cgcaacggcc acggcgctca acccatcggc ctgggcgtgg tttccgtcgc
     9841 cgttcgctga ggctctggcg ttaaatccat cggcgatggc cgtcgacccc tggccggagg
     9901 agaaggcctg cgcggtcccc ttttgcacat gcaccacacc gttggtggag atggccctat
     9961 tttggtcggg ttgtcgaggg ttgtcgcttc tcgcgttcgc atgcagctca ttgggtgagg
    10021 ccgccgccac cccagccgcc catggcgtgg cagccagcgc ggcaccgacc cccaacatca
    10081 ccgctaccgc gccgaccctg ccgattgctg tcgttcccat cgcgcatccc tctctgcaaa
    10141 ccgctgccct gtgcacggat ttgctgttga cgcgcacagc atatggtcgc ccccgcgctg
    10201 ccgataaagt tttcggcgaa ctgcccaacg cgatatgctc gagacttcga taccagtatt
    10261 tcgtatcgca acctgcgtga gtacaaacgt acacgtcaac ggcgcgatgg gagttccgag
    10321 atacactggg catccctcct tgcctgcgcc gaggccaccg tcgccatttt tgcgcaaccg
    10381 ttagattttg tcagtcgaac ggccgactaa acagttagct gtgaatgaat gcaaagcgtc
    10441 gccgaacgaa accgcatgtg gtgcggattg atagccatgc cgccgttcgt gatggctgcg
    10501 aaatccggat caagccggcg gccggaacgg ttcggccgtg cgttccatgc ccgcggcgcg
    10561 gcccttgccc gcgatcacca acgccatctt gcggctggcc tcgtcgatca tctcgtcgcc
    10621 gagcatcact gcaccgcgcg ccccgccgac gcgcgaggtg tgccactcgt aagcctccag
    10681 gatcagctcg gcgtggtcgt aatcggactg ccgcgggctg aaaacctcgt tgcccgccgc
    10741 gatctgttcg ggatgcaata cccacttgcc gtcatagccc agcgccgcgg accgaccggc
    10801 aacccgccgg aaaccctcga cgtcgcggat cttcagatag ggtccgtcga tcgcgaggac
    10861 gccgtgtgtt cgggcggcga tcaggatccg catcagcaca tggtggtgtg cgtcgccgac
    10921 gtcgtagccc tccggctgct cacctacctc gagggtgcgc atgttgaggc tggccgccat
    10981 gtccgccggt cccaggatca gtgcctgcac tcgcgggccg gccgcgatcg cgtcaatgtt
    11041 cgtcagcccc tgtacgtttt cgatctgcac ctcaatcccg atgcggccca acggtaatcc
    11101 gtggttggtc tccagttggc tcagaagtag atccagcgca tggacgtgtg cgacgtcggt
    11161 caccttcggc agcacgatga tgtccagcga tgcgcctgcc gtcgagacca cctcgatgac
    11221 gtccgcatag gtccatggcg ttgtccagtc gttgacccgg accccgcgca gctgttcggc
    11281 ccagcccggc tcggcgagcg cgacggcgac ctgggtgcgg gcctcggcct tcgcatcggg
    11341 cgcgacggcg tcctcgagat cgaggaacac ctcatcggcg gggaggttct ttgccttctc
    11401 gatcatcttg gggttgctgc cgggaaccga aagacaggtt cgacggggcc gatacgcggt
    11461 ttccacagcc atactctcta ctctctaaag acatggcggc ggtgaacagg gtctacgtgg
    11521 cccggctcgc ggggatggtg gtgctgggcc ccgacggaga atccatcggc cgtgtccgcg
    11581 atgtggtgat cagcatcggc gtgtcgggcc atcagccgcg ggttctgggt ctcgtcgtcg
    11641 aattgctcac tcgccgaagg attttcgtgc ccattctgcg ggtgaccgcc atcgaaccga
    11701 gcgccgtcac actcgcgacg ggcaatgtgt ccctgcgccg gttctccaaa cgtcccaacg
    11761 aggtgctggt actcggcgaa gtgatcgaaa cccgggtccg cgtggacgat cccgaactgc
    11821 ccgagctcgc cggaatcgac gccgtcgtgg tcgatctggg catcgagcaa acgcgtactc
    11881 gagattgggt tgtcaccaag gtcgccgtgc gggcgcagcg ccggctcggc cgccgcggca
    11941 acatctccat cgccgactgg aatcgggtgc aaggccttac gccatccgga ctcgcgatgc
    12001 cggaccaggg tgtggcccag ttgctcgaac aattcgaggg ccagcgcccg atcgaggtcg
    12061 ccgaagcgat acgcgagttg cccgcgaagc gccgctacga agtgctcaag gcgcttgatg
    12121 acgagcggct ggccgacgtg ctgcaggagc tgccggaaga cgagcaggcc gacgtgctgc
    12181 ggtcgttgga ggcggagcgc gcggccgacg tgctcgaggc gatggatccc gacgacgccg
    12241 ccgacctgct cggcaccatg accccggccg acgcggagca gttcctgcgc cggatggatc
    12301 ccgaggactc cgaggatgtg cgacggctgc tgagccactc ccccgacacg gcgggcggct
    12361 tgatgacgtc ggaacccgtg gtgctggccc ccgacaccac tgtcgccgag gcgctggcgc
    12421 gagtgcgcga tcccgacctg acgcccgcgg tcgcgtcgct tgtgttcgtc acgcggccgc
    12481 ccacggcgac acccaccggg cactacctcg gctgcgtgca cctgcagcgg ctgctgcgcg
    12541 aacctcccgc cgagctggtt agcggcatcg tcgacaagga cctgccgagc ttgagtcccg
    12601 aggatccgct tgcggcggtg acccgctatt tcgctgccta caatctcgtc tgcggtcccg
    12661 tcgtcgacga cgaaaaccac ctgctggggg cggtgtccgt cgacgacgtc ctcgaccacc
    12721 tgctgcccga cgactggcgg gagcgcgaag aacctgagct gtccggcgcc gaaggggtga
    12781 catgagcgac ttgtcggcgc gtcaacggct ggacacccca cgcaccacgc gccgccgctt
    12841 ttcgctcaac gtcgacgccg acgccgtcgg gcaattcagc gaatcgatcg cccgctttct
    12901 aggcaccggt cgctacctga tgtggcagac catcctcgtc atcgtttgga tcacactgaa
    12961 cctattcgcg gtcaagctgc ggtgggatcc ctatccgttc atcctgctca acctggcgtt
    13021 ctccacgcag gcggcctatg cggcgccgct gatcctgctg gcccagaacc gccaggaaaa
    13081 ccgcgaccgg gtggcgctgg aggaagaccg caggcgtgcc gaacggacga aggccgacac
    13141 cgagtatctg gcgcgcgagc tggccgcgtt gcggctggcc gtcggcgagg tggtcacccg
    13201 cgactacctt cgccgcgaac tcgaggaact acacgagaag ctgaccgaat tgcagcaacc
    13261 gcaaactgcg acagacggcg atgcggcgag taatcccgac agttccgagc gtcggaccaa
    13321 aaggccaggc tgaatcggct ctgcccattg ctgcaacacc cgattcaaga ctaggtatgg
    13381 tgacttggtt cacaagtcac cggcgtacaa ggacggttga gtgcacatag ggggacgagc
    13441 cgccctggag gcagcgcggc gccgagcagg gcgagccgta cgcaagcctg ccttcggcgt
    13501 tgtcatcatc accccgctca tccttgccgg tgccgtcggc gcatccggac catcgccgct
    13561 cacacccgta cgcgattcgg cggtcacgcc actcgcggcg gtcgaacttt cacccggcaa
    13621 ccgatccggc gcatccgtcg tagcggtcac caagacgccg accccgtttc acatcgccgc
    13681 agcgacgagc tccgctcccc cgccctcggt catcgtgaat tcacctggga cacttcgtat
    13741 tccggcgatt gtgttgtccg cgtatcgcaa cgcggagcga atgatggcgg ccgccgcccc
    13801 cggctgcggc gtgagctgga atctgctcgc gggcatcgga cgcatcgaat cgttgcacgc
    13861 caacggtggc gccaccgaca ctcgcggcac cgcggtgcgg ccgatctacg gacccgcgct
    13921 ggacggcacg ctgccgggca acgagatcat cgtgcaaagc cgcaccgccg atcgggtgat
    13981 gtatgcccgc gcgatgggcc cgatgcagtt cctgcccgga acctggtcgc gatacgccag
    14041 cgatggtgac ggcgacggca aagccgatgt gcagaatgtg ttcgatgctt cgctcgcgac
    14101 ggcgcgatac ctctgcagcg gcggcctgaa tttgcgcgat gcgggccaag tgatgtccgc
    14161 gattcttcgc tacaacaact cgatcgccta cgcacgcaat gtgctgggtt gggcggccgc
    14221 atacgcgacc ggtgtccttc cggtggacct gccgccgatt gtcgggtcgg ttccgccgat
    14281 cggcgatttt cacttggaca actacgaggg ccttgggccc ggactgccga tgaacgcgct
    14341 cgggttgcct gccaccgacc cgctggcgat gatgcccatg atggtgaacg gcgatgtcgc
    14401 cagccagatc cccgtctacc caggtcaggc gctcggaccg ctgccagggc ccgcgcaact
    14461 tgttccgcag caggtggcgc agcccgagca gccagcggct ccgccgtggc tgccaccgtg
    14521 gatgcagcag ccggcaccgc agccacagcg accgcccgaa tgtgcggtgt tctgcatcac
    14581 gtctacgcct gctccgaatg cggtcccgcc ggcgccaggt gatgcgcaga tgctgatgcc
    14641 gcaggcgccg cctgcgcccg ccgagccgct aacaccggta ccggcacccg ccgagcagcc
    14701 cgccgccgtc ctcccgccgg caccaggtcc ggcacctggg ccagcgcccg gcccc
//
LOCUS       DASRJA010000548         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1256669,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000548 DASRJA010000000
VERSION     DASRJA010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256669"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1261
                     /gene="mshC"
                     /locus_tag="VFC01_24295"
     CDS             23..1261
                     /gene="mshC"
                     /locus_tag="VFC01_24295"
                     /EC_number="6.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HZN82772.1"
                     /translation="MQSWPAPDVPALPGRGPQLRLYDSADRQVRPVTAGETATMYVCG
                     ITPYDATHLGHAATYLAFDLVYRVWLDSGHAVHYVQNVTDVDDPLFERAQRDGVDWRE
                     LADRETRLFREDMAALRVLPPHDYVAATEAIAEVVELVEKMLASGAAYVVDDPAHPDV
                     YFRADATLQFGYESGYDRDTMLRLFTERGGDPARAGKSDALDALMWRAERPGEPSWPS
                     PFGPGRPGWHVECTAIALSRIGTGLDIQGGGSDLIFPHHEFSAAHAESVTGERRFARH
                     YVHAGMIGWDGHKMSKSRGNLVLVSRLRAQGVKPAAIRLGLLAGHYRSDRSWSDQVLD
                     EAINRLQRWREATALSGGPDASDVLARVRQYLADDLDTPKALAALDGWAADALKYGGH
                     DAEAGRTVATAIDALLGVQL"
     gene            1264..2073
                     /locus_tag="VFC01_24300"
     CDS             1264..2073
                     /locus_tag="VFC01_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HZN82773.1"
                     /translation="MTRALTVFQGRAGDHNDRGFGGAAAVGAEWHRRLGVPATTVGTP
                     QPPLSTDWRAELTAAAPTLEAMAHRYDDIYAAGQTPVTALTRCAVALATLPVVSRYHP
                     DALVVWFDAHGDINTPESSTTGYLGGLALSGPMGLWDSGLGSGIAPANVVLGGARDLD
                     QFERRLIDEGVVRHVPVGPGMVYDLDAAIGDRSVYFHLDCDVLEPGLAPTDYRVPNGL
                     SFDELSRVANRLAENPVVGVEVAEFEGSWSDTGAPGDPRPLLDAVAPLVEG"
     gene            2121..>2731
                     /locus_tag="VFC01_24305"
     CDS             2121..>2731
                     /locus_tag="VFC01_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN82774.1"
                     /translation="MGSVNGKVALITGGANGVGAEVARRLHDKGAKLVLTDIDEALLK
                     EVAAALGEDRVLTVVSDVRDLAAMEGAVAKGVDRFGGIDIVMANAGIATYGSVLKVDP
                     EAFRTLIDVNVVGVFNTVRAALQQVIDRRGYVLIVSSLGAFAAAPGMAPYGVAKAGVE
                     HFANSLRLELAHLGVDVGSAHMSWIDTPLVRDSKADMSAFRDML"
BASE COUNT          419 a          899 c          959 g          454 t
ORIGIN      
        1 tgaacacctt agagtcgacg ctatgcaatc gtggccggct ccggacgtgc cggcgctgcc
       61 gggccgcggt ccgcagctgc ggctctacga cagtgcagat cggcaggtcc gcccggtcac
      121 ggccggcgag acggccacga tgtacgtctg cggcatcacc ccgtatgacg cgacccacct
      181 ggggcacgcc gccacctacc tggcgttcga cctggtgtac cgagtgtggc tggattccgg
      241 gcacgcggtg cactacgtcc agaacgtcac cgatgtcgac gacccactct tcgagcgggc
      301 ccaacgcgac ggcgtcgact ggcgcgagct ggccgaccgt gagacccggc tgttccgcga
      361 ggacatggcc gcgctgcggg tgctgccgcc gcacgactat gtggcggcca cggaggccat
      421 tgcggaggtc gtcgagctgg tcgagaagat gctggcctcc ggcgcggcct acgtcgtcga
      481 cgaccccgcc cacccggacg tctatttccg cgccgacgcc acgctgcagt tcggctacga
      541 gtccggctat gaccgcgaca ccatgctgcg gctcttcacc gaacggggcg gcgaccccgc
      601 ccgcgcgggc aagagcgatg cactcgacgc gctgatgtgg cgggcggagc ggcccgggga
      661 gcccagctgg ccctcaccgt tcggtcccgg tcggcccggc tggcatgtcg aatgcacagc
      721 gatcgcccta tctcgcatcg gcaccggtct ggacattcag ggcggtggca gcgacctgat
      781 cttcccgcac cacgagttct ccgccgcgca cgccgaatcc gtcacggggg agcgacgatt
      841 cgcgcggcat tacgtgcatg ccggcatgat cggttgggac ggccacaaga tgagcaagag
      901 ccgcggcaac ctcgtcttgg tgtcgcgcct gcgcgcccag ggcgtcaaac ccgcagccat
      961 caggctcggc ctgctcgccg gccactatcg cagcgaccgg tcgtggagcg atcaggtgct
     1021 cgacgaggcg atcaaccggc tgcagcggtg gcgcgaagcg actgctctgt cgggaggacc
     1081 ggacgcgagt gacgtgctgg cccgggtgcg ccaatacttg gccgacgatc tagatacgcc
     1141 gaaagcgctt gccgcactgg atggttgggc cgcagatgcg ctcaagtacg gcggccacga
     1201 cgccgaggcg ggccggactg tggcaacagc catcgacgcg ctgcttggcg tccagttgta
     1261 gcgatgacgc gtgccctcac cgtctttcag ggtcgcgccg gcgaccacaa cgatcgcggt
     1321 ttcggcggcg cggcggcggt aggtgcggag tggcaccggc ggttgggcgt gcctgcgacc
     1381 accgtcggca cgccgcaacc accgctgagc accgactggc gcgcggagct gacggcggcc
     1441 gcgccgacac tggaggcgat ggcacaccgg tacgacgaca tctacgccgc aggacagacg
     1501 ccggtgaccg cgttgacccg ctgcgccgtc gcgctggcca ccctgcccgt ggtgtcccgg
     1561 tatcacccgg atgccctggt ggtgtggttc gacgcgcacg gcgacatcaa taccccggag
     1621 tcgagcacga ctggctacct cggcggcctc gcgctgtccg gcccgatggg tttgtgggac
     1681 tccgggctcg ggtcgggcat tgcgcctgcg aacgtcgtgc tgggcggggc acgcgacctg
     1741 gatcagttcg agcgccgtct catcgacgag ggtgtggtgc gacacgtgcc ggttggcccc
     1801 ggcatggtgt atgacctcga cgcggcgatc ggagaccggt cggtgtattt tcacctcgac
     1861 tgcgacgtgc tcgaacccgg tttggcgccc accgattacc gggtcccgaa cgggctgagc
     1921 ttcgatgaat tatccagggt ggcaaaccgt ttggcggaaa accccgtcgt cggggtcgag
     1981 gtcgccgagt tcgaaggcag ctggtccgac accggcgcgc cgggtgatcc gcggccactc
     2041 ctggatgccg ttgcgccgct cgtcgagggc tgattgtcaa ttcggtaagc cgacccgccc
     2101 ggtggagtac gttgaccgcc atgggttctg tgaacggaaa agtcgcgctg atcaccggcg
     2161 gcgccaacgg tgtcggcgcc gaggtggcgc gccgcctgca cgacaagggc gccaagcttg
     2221 tgctgaccga catcgacgag gcgctgctta aggaggtggc ggcagcgctc ggcgaggatc
     2281 gagtgctcac ggtcgtctcc gatgtgcgcg atttggcggc catggaaggc gccgtcgcaa
     2341 agggcgtcga ccggttcggc ggcatcgaca tcgtgatggc gaacgccggg attgcgacgt
     2401 atggctcggt actgaaggtc gatcccgagg cgtttcgaac gctgatcgac gtcaatgtcg
     2461 tcggggtctt caacaccgtt cgcgccgcgt tgcagcaggt gatcgatcgg cgcggttacg
     2521 tgttgatcgt gtcgtcgctt ggggcctttg ccgcggcccc ggggatggcg ccttacggtg
     2581 tggccaaggc aggcgtcgag cacttcgcca actcgctgcg cctcgagctc gcacatctgg
     2641 gcgtcgatgt cggctctgca cacatgtcgt ggatcgacac tccactcgtc cgcgatagca
     2701 aggccgacat gtctgccttc cgggacatgc t
//
LOCUS       DASRJA010000549         5786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3295453,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000549 DASRJA010000000
VERSION     DASRJA010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3295453"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="VFC01_24310"
     CDS             <1..125
                     /locus_tag="VFC01_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82775.1"
                     /translation="LARPPNLPPPDVPPCPGPTGERADWWWYEPFQPQPPPSIN"
     gene            complement(218..463)
                     /locus_tag="VFC01_24315"
     CDS             complement(218..463)
                     /locus_tag="VFC01_24315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82776.1"
                     /translation="MQFSAVSYLAIEAKYLETQAPVSKAQRPTDKLSTATAPGLVVVA
                     VGVEDLIDAPGQRFDPTWLTLVRTSRGRDEGVADVVG"
     gene            633..986
                     /locus_tag="VFC01_24320"
     CDS             633..986
                     /locus_tag="VFC01_24320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82777.1"
                     /translation="MPGRTNDFQELITLLVQTIGEDRATPSAMVRSKVPGVGKREVDI
                     RVEAEVDGHPIYIGIEVSASKSRKKTVEWVERMQGKHTHLSTSKLVLVSSSGFTKAGW
                     RWPRTTASMRSPRVR"
     gene            935..1633
                     /locus_tag="VFC01_24325"
     CDS             935..1633
                     /locus_tag="VFC01_24325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82778.1"
                     /translation="MALAAHYGIDAITPGEVTPGFVGEIVNNLKSFWLERLDFTAEET
                     TVYFDPPIDSPEGPLDHFTFPREAWGVHVGTADESVASSVGEMVGAALHNLDLSKGNL
                     HDLAPGEGVPFNSEHDGPVTIAGEPLFLRGGSGVEPHSLRRITKVDIGGKLNKAGIEM
                     PLQHGEFDGSHYSNGAVVLGDHKFHSAVVEGEDGPRFGMRVAPVDKPLDARSYRGRGS
                     GKKLVIVPEGSSDT"
     gene            complement(1656..1898)
                     /locus_tag="VFC01_24330"
     CDS             complement(1656..1898)
                     /locus_tag="VFC01_24330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82779.1"
                     /translation="MIPRHANYLQLMEDFPAEELRTVKPSPVTVQRPPLKANADPELT
                     REFLVRAENLGITKDAALDQALADWIAGNPETGSGS"
     gene            complement(1922..2767)
                     /locus_tag="VFC01_24335"
     CDS             complement(1922..2767)
                     /locus_tag="VFC01_24335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82780.1"
                     /translation="MATQTAIERWDKVLDAVEKSRESWLYPEAMTPEVRKHQYSRDLP
                     MIIETVFSQFVHAWNTDRPDQGSDLYQVLEPIARAIAAPLDFTRAGLAQVDPMMAAVA
                     EARSGLQQIGGPATAAEVVADIEHLLKVTVLVARVSHQGSTKIIDDELQARFDAINKN
                     KARSAKYLDLHTFQAVEQPSKTTISLQVVLSMIDPGIATLEERMNPFPEETYPPIMKQ
                     FAAQLGGELLHRVGRAFSRAAGPRPRLRDKRRTIGLPSVISARCDTITCTTAGSAETR
                     PRTRC"
     gene            2818..3114
                     /locus_tag="VFC01_24340"
                     /pseudo
     CDS             2818..3114
                     /locus_tag="VFC01_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease"
     gene            complement(3121..5313)
                     /locus_tag="VFC01_24345"
     CDS             complement(3121..5313)
                     /locus_tag="VFC01_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-like protein EF-G2"
                     /protein_id="HZN82781.1"
                     /translation="MADKTTTSQGAGAAPTADSPAAIHNVVLVGPSGGGKTTLVEALL
                     VASGVLTRQGSVVEGNTICDFDEAEISQQRSVGLALASLEHDGIKVNLIDTPGYLDFV
                     GELRAGLRAADCALFVIAANEGVDDPTKSLWLECAQVGMPRAVVVTKLDHARANYESA
                     LTAAQEAFGDKVLPLYLAADTPCTGLIGLLSQTHYEYADGKRTASHAPDGSYSDAIEE
                     MRGTLIEGIIEESEDETLMERYLGGEEIDQSGLIDDLEKAVARGSFFPVIPACSGTGV
                     GTMELLEIITSAFPSPPEHQLPEVFTPQGKARDGLACDPNGPLLAEVVKTTSDPYVGR
                     VSLVRVFSGTIRPDATVHVSGHLSQFFGSDGFLSSSATHADSGSLAGHADHDEDERVG
                     TLSFPLGKQQRPAPSVVAGDICAIGKLSRAETGDTLSDKSDPLVLKPWTMPEPLLPVA
                     VQPRAKTDEDKLSVGLTRLAAEDPTLRIEQNPETHQIVLWCMGEAHTGVVLDALARRY
                     GVAVDTVDLRVPLRETFGGNAKGHGRHVKQSGGHGQYAVCDIQVDPLPEGTGFEFVDK
                     VVGGAVPRNFIPSVEKGVRAQMEKGVSAGYPVVDIRVTLFDGKAHSVDSSDFAFQMAG
                     GLALREAAAATRVNLLEPVDVVTVVVPDDMVGSVMGDLAGRRGRVVGTDKVGEDRTMI
                     KAEIPEVELTRYAIDLRSLSHGAGSFTRSFARYEPMPESAAAKVKTSA"
     gene            5436..>5786
                     /locus_tag="VFC01_24350"
     CDS             5436..>5786
                     /locus_tag="VFC01_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZN82782.1"
                     /translation="MTTVTADIAVSLGGYAAGPNECRPAVDCRLHTWMVDHADENRAA
                     IDAITSAGAYIMGRNMFGPGRDAWDLEWKGWWGPEPPYHAPVFVLTHHPREPLEMDGG
                     TTFTCVTDGPESALA"
BASE COUNT         1018 a         1938 c         1805 g         1025 t
ORIGIN      
        1 cgctcgcgcg tccgcccaac cttcccccac ccgatgtccc accgtgcccc ggacccaccg
       61 gtgagcgcgc cgactggtgg tggtatgaac ccttccagcc gcagccacca ccgtcaatca
      121 actaggttgg ggcatagaga atttcgttcg cgatacgcgc agctaccgca tcgtgaaatt
      181 cgaagctgac aaccagaccc gcaccgccgc cgacccgcta accgacgacg tccgcaacgc
      241 cctcatcacg accgcgcgac gtgcgcacca acgtgagcca agtcgggtca aagcgctggc
      301 ccggcgcatc gatgagatct tcaaccccga cggcgactac gacgaggccc ggtgctgttg
      361 ccgtcgacag cttgtcggta ggtcgctgag ctttgctcac aggagcctgg gtctccaggt
      421 acttcgcttc gatcgccagg tacgaaaccg cgctgaactg cactttcgca ggcctttttt
      481 gggtcgccgc agtctaaggt caaagcgcgc cgagaccgac tcggcaacac ttccagcaac
      541 caagtcattg ggaaacttct tggaactctg cgcgtggtga ctggcccgga caaaccccgt
      601 gacctgtcag tgtcgaccgg tacgttgcga gtgtgcccgg acgcaccaac gatttccaag
      661 aactgatcac cctgctcgtt cagaccatcg gtgaagaccg agcgacgccc tccgcgatgg
      721 tgagaagcaa ggtcccgggt gtgggcaagc gcgaggtcga catccgcgtg gaggcggagg
      781 tagacggcca cccgatctac atcggtatcg aagtcagcgc gtcgaagtcg cgcaagaaga
      841 cggtcgagtg ggtcgagcgc atgcagggaa agcacaccca tctctccaca tcgaagctcg
      901 tgctcgtctc ctcgagcggt ttcaccaaag cgggttggcg ctggccgcgc actacggcat
      961 cgatgcgatc accccgggtg aggtgacccc gggcttcgtc ggggagatcg tgaacaacct
     1021 gaagagcttc tggctggaga ggctcgactt cacggcggag gaaacgaccg tctacttcga
     1081 tccgccgatc gactcgccgg agggtcccct cgaccacttc acgttccccc gcgaggcctg
     1141 gggtgtgcat gtcggcaccg ccgatgaatc ggtggcttcc agcgttggcg agatggtagg
     1201 ggccgcgttg cacaatctcg acctcagcaa gggaaacctg cacgatttgg caccaggcga
     1261 gggggtcccc ttcaacagcg agcatgacgg cccggtgaca attgcggggg agccgttgtt
     1321 ccttcgcggg ggcagtgggg tcgagccgca cagcctgcgc cggattacca aggttgatat
     1381 cggcgggaag ctaaacaagg ccggaattga aatgcccctt cagcatgggg aattcgacgg
     1441 cagtcactac tcgaacggcg ccgtcgtgct gggcgatcac aagttccact cggccgtcgt
     1501 tgaaggcgag gacggccctc gctttggaat gcgcgtcgcc ccggtggaca aaccgctcga
     1561 cgcccgctcc taccggggca ggggtagtgg caagaaattg gtgatagtcc cggagggcag
     1621 ctccgacacg tgaacgcctc tatcgggcga cgacgctagc tgcccgatcc ggtctctgga
     1681 ttacccgcga tccaatcggc gagcgcctga tcgagcgccg cgtccttggt gatgcccagg
     1741 ttctcggccc gcacgaggaa ctcgcgggtc agctccggat cggcgttagc tttcaacggc
     1801 gggcgctgga cggtgacggg cgagggtttg accgtgcgca gctcctcggc ggggaagtcc
     1861 tccatcagct gcagatagtt cgcgtgcctg ggaatcatcg tgtctccctt gcgataccac
     1921 ttcagcacct tgttcttggc cgagtttctg cagatcccgc ggttgtgcac gtaatcgtgt
     1981 cgcatcttgc cgagatcacc gaaggtagtc cgatcgtacg tcgcttgtct cgcagccgag
     2041 ggcgagggcc agctgctctc gaaaatgctc gtcccactcg gtgtaaaagt tcaccaccca
     2101 actgagcggc gaactgcttc attatcggcg ggtacgtctc ctccgggaac gggttcatcc
     2161 gctcctccag ggtggcgatt cccggatcaa tcatcgacag gacgacctgc aggctgatgg
     2221 tggtcttgga tggctgctcg acggcctgaa acgtatgcag gtcaaggtat ttcgcgctac
     2281 gggccttgtt cttgttgatg gcatcgaacc gggcctgcag ctcgtcgtcg atgatcttgg
     2341 tcgagccttg atggctcacc cgagcgacca gcaccgtgac cttcaataga tgctcgatgt
     2401 cggcgaccac ttccgcagcg gtcgcgggtc cgccgatctg ttggagcccg gatcgtgcct
     2461 cagcgactgc cgccatcatg gggtccacct gcgccagccc ggcccgagtg aagtccaacg
     2521 gcgcggctat ggcgcgggct atcggttcga ggacctggta aaggtccgag ccttggtcgg
     2581 gccggtcggt gttccatgca tgcacgaact gcgagaacac ggtctcgatg atcatcggta
     2641 ggtctcgtga gtattgatgc ttcctgactt ccggcgtcat cgcctcgggg tagagccagc
     2701 tctcgcggga tttctctacc gcgtccagca ccttgtccca acgctcgatc gccgtctgtg
     2761 tcgccatgct gggtacggta tccagaagcc gcgacatcta cggtgtacca acccgcatgt
     2821 cattcccgcg acggcggctg cacccgcccc aactgcaccg cgccgggcta ctactccgag
     2881 gtacaccaca gccccgccgg ggccgccggc ggccgaaccg acgccgacgc cctgttcttc
     2941 gcctgcggcc ccgaccacaa actcatcacc gacggccact accaaaccga agtcaccgac
     3001 accggccgac tggcctggac cgacggcacc acaccaccgg aaatcaacca cgcccaccac
     3061 cccgaagaac tgctgcgcgg caacaccgac ccacccgaag atgaagacgg atgacgcgcc
     3121 tcaggcggac gtctttacct tcgccgccgc tgactccggc atgggttcgt agcgcgcgaa
     3181 ggaccgggtg aacgatccgg cgccgtgcga caacgaccgc aaatcgattg cgtagcgggt
     3241 caactcgacc tcggggatct ccgccttgat cattgtgcgg tcctcgccga ccttgtcggt
     3301 gcccaccacg cggccccgtc gcccggccag atcacccatc accgagccga ccatgtcgtc
     3361 gggcaccacg accgtcacga catcgaccgg ctcgagcagg tttacccggg tcgccgcggc
     3421 tgcctcgcgc agcgccagcc cgccggccat ctggaatgcg aagtccgacg agtccacgct
     3481 gtgggccttg ccgtcgaaca gcgtgacccg gatgtctacc accggatagc ctgcgctgac
     3541 gcccttctcc atctgagcgc ggacgccctt ctcgacgctg ggaatgaagt tgcgtggtac
     3601 cgcgccgccg accaccttgt cgacgaactc gaacccggtg ccttccggca gcgggtccac
     3661 ctggatgtcg cacaccgcgt actggccgtg gccgccggat tgtttgacgt ggcggccgtg
     3721 tcctttggcg ttgccgccga acgtttctcg cagcggtacc cgaagatcga cggtgtccac
     3781 cgccacgccg taccgccggg ccagcgcgtc aagcaccaca cccgtatggg cctcgcccat
     3841 gcaccacagc acgatctggt gggtttcggg gttctgctcg atgcgcagcg tcgggtcctc
     3901 ggcggcgagt cgcgtcaacc cgaccgagag cttgtcctcg tcggtcttgg cgcgtggctg
     3961 cacggcgaca ggcagcagcg gctcgggcat cgtccacggc ttgagcacca gcggatcgga
     4021 cttgtcggac agcgtgtcgc cggtctcggc cctactcagc ttgccgatcg cgcagatgtc
     4081 gccggccacc acggacggtg ccggccgctg ttgcttgccg agcgggaacg acagcgtgcc
     4141 gacgcgttcg tcttcgtcgt gatcggcatg gccggccaac gaaccggagt cggcatgggt
     4201 ggccgacgag gacaggaaac cgtcggagcc gaagaactgc gagagatggc ccgacacatg
     4261 cactgtcgcg tcgggcctga tggtcccgga gaacacccgg accagactca ccctgccgac
     4321 gtagggatca gacgtcgtct tgaccacttc cgcaagcagc ggtccattcg gatcgcaggc
     4381 cagcccgtcg cgggccttgc cctgcggcgt gaaaacctcg ggcagctggt gttccggggg
     4441 cgacggaaac gcgctagtga tgatctccag cagttccatc gtgccgacgc cggtgccact
     4501 gcatgccggg atcaccggga agaacgaccc tcgggcgacc gccttctcca ggtcgtcgat
     4561 cagccccgac tggtcgatct cttcgccgcc gaggtagcgc tccatcagcg tctcgtcctc
     4621 ggactcttcg atgattccct cgatcagcgt gccgcgcatt tcctcgatcg cgtcggagta
     4681 cgacccgtcc ggtgcatggg acgcggtgcg tttgccgtct gcgtattcgt agtgcgtctg
     4741 ggacagcagc ccgatcagac cggtgcaggg cgtgtcagcg gccagataaa gcggaagcac
     4801 cttgtcgccg aacgcttcct gggccgccgt gagcgcgctc tcgtagttgg cgcgggcgtg
     4861 gtcgagcttg gtgaccacca ccgcgcgcgg catgcccacc tgagcgcact ccagccacag
     4921 cgacttggtc ggatcgtcga caccttcgtt cgcggcgatc acgaacaatg cgcagtccgc
     4981 ggcccgcagc cccgcgcgca gctcaccgac gaagtcgaga tagccgggcg tatcgatgag
     5041 gttgaccttg atcccgtcgt gctccagcga cgcgagcgca aggccgaccg accgctgctg
     5101 ggagatctct gcttcatcga agtcgcaaat ggtgttgccc tcgaccaccg agccctgtct
     5161 ggtgagtacc ccggaggcga cgagtagcgc ctcgacgagt gtcgtcttgc cgccacccga
     5221 tgggcccacc agcaccacat tgtggatggc ggccggactg tccgcggtgg gggcggctcc
     5281 ggcgccctgg gaagttgtcg tcttgtcagc catgacattc cctcctcatc cggacggcca
     5341 ccctgccgcc ggatgtgtta atccacaatt ccccgaacag acaccgagca caagcgattc
     5401 gatgactttc tggcggggcg gaagtcagtg ccggcatgac cacagtcacc gcagacatcg
     5461 ccgtctcgct cggcggctac gccgcaggtc cgaacgagtg ccgacctgcc gttgactgcc
     5521 ggttgcacac ttggatggtc gaccacgccg acgagaaccg cgccgcgatc gatgcgatca
     5581 cctccgcggg tgcctacatc atgggccgca acatgttcgg gcccggccgc gatgcgtggg
     5641 atctcgagtg gaagggatgg tggggacccg agccgccgta tcacgcgcca gtgttcgtgc
     5701 tcacccacca tcctcgcgag ccgctggaga tggacggcgg cacgaccttc acgtgcgtaa
     5761 ccgatgggcc ggagtccgcg ctggcg
//
LOCUS       DASRJA010000550         4884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1679926,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000550 DASRJA010000000
VERSION     DASRJA010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679926"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VFC01_24355"
     CDS             complement(<1..526)
                     /locus_tag="VFC01_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007301794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN82783.1"
                     /translation="MSGGPTLKQGLSERQLRMIAIGGVIGAGLFVGSGVVIGSTGPGS
                     FITYALCGFLVIMVMRMLAEMAVANPSTGSFADYARSALGGWAGFSVGWLYWYFWVII
                     VGFEAIAGAKIVQYWLPGIPLWLSSLVLLSLMTLTNLFSVSSFGEFEFWFAGIKVAAI
                     IVFLTVGTAFVFGLW"
     gene            1135..3597
                     /locus_tag="VFC01_24360"
     CDS             1135..3597
                     /locus_tag="VFC01_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005252394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN82784.1"
                     /translation="MAAPRVVIIGAGIVGANLADELTARGWDQVTVVDQGPLPLTGGS
                     TSHAPGLVFQTNASKTMAEFASYTVEKFVGMDLDGEWCFNQVGGLEVATTPERLAELH
                     RRQGWATSWGIEGSLIEPEECARIHPLVDESKILGGLYVPSDGLAKSTRVVVALARRA
                     QARGAVFRGSIKVTGIERGGGRVTGVATSEGTIPADIVVSCAGFWGPEIGAMVGMDIP
                     LLALAHQYVKTDQIPDLVGRNTEAAEAGLPILRHQDQDLYFREHVDRLGIGSYAHRPM
                     PVDLRDLTDSADVSAASMPSMLPFTDEDFAQPWQQCKLLLPCLEKAKVESGFNGIFSF
                     TPDGAPLVGESSDVAGFWIAEAVWVTHSAGVARAVAQLLVDGRSDTDLHGCDVHRFEE
                     VQLAPEYVSETSQQNFIEVYDILHPLQPRLSPRDLRVSPFNVRQQELGAVFLESGGWE
                     RPHWYEANARLLQQLPPEWAPPARDAWAGQFHSPIAAAEAWRTRKSVAMYDMTSLKRL
                     EITGRGALPLLQRLTTGKMDKSVGSVTYTLALDEAGGIRSDLTVARLAADHFQVGANG
                     NIDLDYFRRQAAGDDTVQVRDITGATCCIGLWGPRARDVVQALSRDDFSNENFKYFRT
                     KPVRIAGIPVTAMRLSYVGELGWELYTSAEHGLRLWDVLWAEGQKHDVIAAGRAAFNS
                     LRIEKGYRAWGTDMTTEHNPYEAGLGFAVSAKKTDFVGHEALQGISDSTIKRRLMTVI
                     VDDGVSVVMGNEPVFLGDKPVGYVTSAAFGHTVGAPVAYAWLPASANEGTRIEIQYFD
                     RRIAATVAPEPLVDPEMTRIRS"
     gene            complement(3600..4226)
                     /locus_tag="VFC01_24365"
     CDS             complement(3600..4226)
                     /locus_tag="VFC01_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN82785.1"
                     /translation="MSLRIERVVTKGTFALDGGTWEVDNNIWLVGDDSDVVIIDAAHE
                     AAPIVDAVAGRNVTAVICTHGHNDHVTVAPELGERLHCPVLLHPGDDVLWKMTHPEEP
                     YWRLDDGQRIDVAGTEIQIIRTPGHSPGSCCLYVPEAGVLFSGDTLFSGGPGATGRSF
                     SDFPTIVDSIRDRLFTLPDDTRVHTGHGDGTTIGTEAPHLAEWIARGH"
     gene            complement(4223..>4884)
                     /locus_tag="VFC01_24370"
     CDS             complement(4223..>4884)
                     /locus_tag="VFC01_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN82786.1"
                     /translation="GQCTKVDPNADPAVVGLLGCGVMAGLGAAMNTGNVSRGDSVAVI
                     GCGGVGDAAIAGARLAGASPIIAIDRDPKKLRMATELGATHTIDASKVDAVEAVQELT
                     GGFGADVVVDAVGRPETWKQAFYARDLAGTVVLVGVPTPDMTLEMPLIDFFSRGGALK
                     SSWYGDCLPERDFPALVDLYEQGRLPLDKFVSERIKLDQVEDAFATMHRGEVLRSVVV
                     L"
BASE COUNT          934 a         1609 c         1518 g          823 t
ORIGIN      
        1 gccacaaacc gaacacgaag gccgtgccca cggtgaggaa cacgataatc gcggcgacct
       61 tgatgccagc gaaccagaat tcgaactcac caaaggatga aaccgagaac aggttggtca
      121 gggtcatcag gctcaacaga accagcgaag acagccacag cgggataccg ggcagccagt
      181 actgaacgat cttggctccg gcgatggcct cgaacccgac gatgatcacc cagaagtacc
      241 aatagagcca gccgaccgag aatcccgccc aaccgcccag cgcgctgcgg gcataatcgg
      301 cgaacgatcc cgtcgacgga ttggccaccg ccatctccgc cagcatccgc atcaccatga
      361 tgaccaggaa tccgcacaac gcgtacgtga tgaacgaacc cggcccggtg ctgccgatga
      421 ccacgcccga gccgacaaac aggccggctc cgatcacgcc gccgatggcg atcatcctca
      481 gctggcgctc gctgagacct tgcttgagag ttggcccccc gctcatcgcg cgtctccttg
      541 aacaggaacc gataacactc cacccgcgaa agacgaaacc tgcggcctaa caatttcgga
      601 tgcctcggcc cgcggccagc cgctcgcctc gaaccgacgg gccggaccag atgccccact
      661 ccacgatccg gttggatcac tcatcgttgt cacgtcaacc tccctgcagc gtgtatcgat
      721 tgcgtcttgg accagcagct ctgatagccc gaccttgtgt cccgaacgga atgaggttgc
      781 gtattaaaca acctggtgct caatgtggaa cacattcgga acaccgtagt gtgataaatc
      841 acaatgcagc gtcaaactgt gatctagatc acagctgtga aacgcctgtc aaccgcccgc
      901 tggacacgac ctcggggaga catcgaatgc cgctgtgagc gtccaaagtc gcggctgagg
      961 caacgcgggc gtcggcgatg cgagcgaggg gttgacccac ccttatccac ttaatatggt
     1021 tctgatatat cagttgtcaa ccacagtctt cggcgtcttc gtttccgtta acgacgcctg
     1081 gtgcgcgcca ggcagtaaac cgacgcgcta gcccgcccca actaagagga gatcatggcc
     1141 gcaccgcgtg tagtaatcat cggcgccggc atcgtcggcg cgaatctggc cgatgaactc
     1201 accgcacgcg gctgggatca ggtcaccgtg gtggaccaag gtcccttgcc gctgacgggc
     1261 ggctctacgt cgcacgcccc gggtttggtt ttccaaacca atgcgtcgaa aacgatggcg
     1321 gagttcgcca gctacacggt cgagaagttc gtcggcatgg atctcgacgg cgagtggtgc
     1381 ttcaaccagg ttggcggtct ggaggtggca acgacgcccg agcggcttgc cgagttgcat
     1441 cgtcggcagg ggtgggcgac gtcgtggggc atcgagggct ccttgatcga acccgaggaa
     1501 tgcgcacgga ttcatccgct cgtcgacgaa tcgaaaatcc ttggcggact gtatgtgccg
     1561 agcgacgggc tcgccaaatc gacgcgtgtg gttgttgcgc ttgcacggcg cgcccaggcc
     1621 cgcggcgcag tattccgcgg atccatcaag gtgactggaa tcgaacgcgg cggcggacgc
     1681 gttaccggcg tcgcgacgtc ggaggggacg attcccgccg acatcgtggt ctcctgcgcg
     1741 gggttctggg gcccggagat aggtgcgatg gtgggcatgg acatcccgct attggccctc
     1801 gcgcaccaat acgtcaagac cgaccagatt ccagatcttg tcggccgcaa caccgaagcc
     1861 gcggaggccg gcctgccgat cctgcgtcac caggaccagg acctgtactt ccgcgaacac
     1921 gtcgaccgtc tcggcatcgg ctcctacgcg caccgcccga tgcctgtgga cctgcgcgat
     1981 ctgaccgact cggctgacgt gtccgcagcg agcatgccgt cgatgctgcc cttcaccgac
     2041 gaggatttcg cacaaccctg gcagcagtgc aagctattac tcccctgcct cgaaaaggca
     2101 aaggtcgaaa gcggattcaa cgggatattc tccttcaccc ccgacggtgc gccgctggtc
     2161 ggcgagtcct ccgacgtcgc tgggttctgg atcgccgagg ccgtctgggt gacacactcg
     2221 gcgggcgtcg cacgcgccgt cgcgcaacta ctggtcgacg gacgcagcga caccgatttg
     2281 cacggctgcg atgtgcaccg cttcgaagag gttcagctcg cgcccgagta cgtcagcgag
     2341 acgtcgcaac aaaacttcat cgaggtctac gacatcctgc atccgctgca gccacgactg
     2401 tcgccgcggg acctccgggt cagccccttc aacgtcagac agcaggagct cggcgcggtg
     2461 ttcctggaga gcggtggctg ggagcgtccg cactggtatg aggccaatgc acgtctgctc
     2521 caacaacttc cacccgagtg ggcgccgccc gcccgcgatg catgggccgg gcagtttcac
     2581 tcaccaatcg cggcggccga agcgtggcgc accaggaaat cggtcgcgat gtatgacatg
     2641 acgtcactca agcggctcga gatcaccggc cgcggcgccc tgccgctcct gcagcgtctg
     2701 accaccggca agatggacaa gtccgttggc tccgtcacct acacgcttgc actcgacgag
     2761 gccggcggaa tccgcagcga cctcaccgtt gcccggttgg ccgcggacca tttccaggtc
     2821 ggggcgaacg ggaacatcga tctggactac ttccgtcgac aggcagcagg cgacgacacc
     2881 gtccaggtcc gcgatatcac aggggcgacc tgctgcatcg gcctgtgggg tccgcgcgca
     2941 cgcgacgtgg tccaggccct cagccgcgac gacttctcga acgagaactt caagtacttc
     3001 cgcacgaaac ctgtacggat cgcgggtatt ccggtcacgg ccatgcgcct gtcgtatgtc
     3061 ggtgaactcg gatgggagct ttatacgagc gccgagcacg ggctgcgtct gtgggatgtg
     3121 ctgtgggctg aaggccaaaa gcacgatgtg atcgccgccg gacgggcggc cttcaacagc
     3181 ctgcgcatcg aaaagggcta ccgggcatgg ggtacggaca tgacgaccga acacaacccg
     3241 tatgaggccg gccttggctt cgccgtatcc gccaagaaga cggacttcgt cggacacgag
     3301 gcgctgcaag gcatttcgga ttcgacgatc aagcgccgcc tcatgacggt gatcgtcgac
     3361 gacggcgtgt cggttgtgat gggcaacgag cctgtcttcc tgggcgacaa gccagtcggc
     3421 tatgtcacca gcgcggcatt cggccacacc gttggtgcgc cggtggccta cgcctggctg
     3481 ccggcctccg ccaacgaagg cacccggatc gagatccagt acttcgaccg gcggatcgcg
     3541 gccacggttg cacccgaacc gctcgtcgac cccgagatga cgcggatcag gtcctagcgt
     3601 tagtgccctc gcgcgatcca ttcggcgaga tggggcgcct cggttccgat agtggtgccg
     3661 tcgccgtgac cggtgtgcac ccgggtgtcg tcgggcagtg tgaacagccg gtcgcggatg
     3721 gagtccacga tcgtcggaaa gtccgagaat gaccgtccgg tggcgccagg tccgccgctg
     3781 aacaaggtgt cgccggagaa cagcacaccg gcctcaggca cgtacaggca gcacgacccc
     3841 ggtgagtgcc cgggggtgcg gatgatctga atctccgtgc ccgccacgtc gattcgctga
     3901 ccgtcatcga ggcgccagta cggctcctcg ggatgggtca ttttccacag cacatcgtcg
     3961 cccggatgca gcagcaccgg gcagtgcaac cgctcgccaa gctccggagc gacggtcaca
     4021 tggtcgttgt ggccgtgagt gcagatgacc gcggttacgt tgcggcctgc gacggcgtcg
     4081 acgatcggcg cggcctcgtg tgcggcatcg atgatcacca cgtcgctgtc gtcaccgacg
     4141 agccagatgt tgttgtcgac ctcccacgtg ccaccgtcca gcgcgaaagt tcccttggtg
     4201 acgacacgct cgatgcgcag gctcacagca cgaccaccga ccgcagcacc tcgccgcgat
     4261 gcatggttgc gaatgcgtcc tcgacctggt ccagcttgat gcgctcgctg acgaacttgt
     4321 ccagcggcaa gcggccctgc tcataaagat cgacgagcgc cgggaagtca cgctcgggca
     4381 ggcagtcgcc gtaccacgat gacttcagcg cgccgccgcg cgagaagaag tcgatgagcg
     4441 gcatctccag cgtcatgtca ggagtcggca cgcctaccag gacgacggtg cccgcgaggt
     4501 cgcgggcata gaaggcctgc ttccaggttt ccgggcggcc caccgcgtcg acgacgacat
     4561 cggcgccgaa gccgccggtc agttcctgca ctgcctccac cgcgtccacc ttcgacgcgt
     4621 cgattgtgtg ggtggcgccc aattccgtgg ccatccgcag cttcttgggg tcgcggtcga
     4681 tggcgatgat cggtgacgcg ccagcaagcc gcgccccggc gatcgccgcg tcgcccaccc
     4741 ctccgcagcc aatcactgcg acggagtctc cgcgcgagac gttgccggtg ttcatcgccg
     4801 cgcccaggcc ggccatcaca ccgcagccga gcaacccgac gacggcgggg tcggcattcg
     4861 gatccacctt cgtgcactgt cctt
//
LOCUS       DASRJA010000551         8409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3906058,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000551 DASRJA010000000
VERSION     DASRJA010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3906058"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VFC01_24375"
     CDS             complement(<1..901)
                     /locus_tag="VFC01_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZN82787.1"
                     /translation="MIAGAPPALAFPALSWWWLAWVGLVPLLLVVRAAPTAMQGAVRA
                     WAGMTGFVLITQYWLWPAAGPLLIVLAVGLGTLWLPWGWAAHRLLSGELTIWRVVAAV
                     VVLPSWWVLAEAARSWDKLGGPWTLLGASQWNQPATLAPASVGGVWLVSFLIVAVNTA
                     VVGALLVRTAWLVALPIALAIVSPAWFWWGPAPAEGSSVRIALVQPGDIEDATARQAA
                     GEALTETLAGQQLDLVVWGESSVGDDLGSHPDAMARLVELRRRIGADLLVNVDARAPS
                     GGIYKSSVLIGSSGARGSYSKTRL"
     gene            complement(927..1811)
                     /locus_tag="VFC01_24380"
     CDS             complement(927..1811)
                     /locus_tag="VFC01_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HZN82788.1"
                     /translation="MNNDQFNKAKNGAGFIAALDQSGGSTPKALKLYGIPEDAYSGDE
                     QMFDLVHEMRTRIITSPAFNGDRIMGAILFEMTMDRQIEGRPTADYLWNVKNIVPFLK
                     IDKGLADEQDGAQTMKPMPGLDDLLDRAVANGVFGTKERSVIKLPGAGLDAVVAQQFE
                     VGQQVLAKGLVPIIEPEVDIKSPRKAEAEDQLNAALLDGVNALGDDQKVMLKLTLPDT
                     DNLYKQLVDHPKVLRVVALSGGYSRDEACEKLARNTGVIASFSRALTEGLTAQQSDEE
                     FNATLDQAIASIAKASAT"
     gene            1878..3185
                     /locus_tag="VFC01_24385"
     CDS             1878..3185
                     /locus_tag="VFC01_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN82789.1"
                     /translation="MTATFSKTPIAPRRNGAPPPDVPLADIDLGTVEFWNLDDDLRDG
                     AFATLRRESPITFFEVPEVAGFAPGAGHWALTTYDDVHHASRHPEIFSSIPTSTALND
                     VPAEIAEFAGSMISLDDPRHLRLRKIVNRAFTPKVVGRIEDSVRDRARRLVTDMITNH
                     PDAHADFVAEVSGPLPLQIICDMMGIPEEDEENVFHWTNVMLGVGDDEASGEFDEVIK
                     TVTEIAEYGVALAEERRARPGDDLTTNLVQADVDGERLTSVEIASFFILLSAAGNETT
                     RNAISHGMVALTRYPEQRETWWSDFDGFAATAVEEIVRWASPVIFMRRNLTEDIEMGG
                     IPMKAGDKVSMWYNSANRDERKFTNPWLFDLTRDPNPHVGFGAGGAHFCLGANLARRE
                     IRVLFSELYRQIPDVVAVQEPAILHSAFIHGIKRLPVEWTPPN"
     gene            complement(3192..3377)
                     /locus_tag="VFC01_24390"
                     /pseudo
     CDS             complement(3192..3377)
                     /locus_tag="VFC01_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003408088.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(3489..3716)
                     /locus_tag="VFC01_24395"
     CDS             complement(3489..3716)
                     /locus_tag="VFC01_24395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82790.1"
                     /translation="MSREPTDIAAEARTLLDALANSTDPAAFQTLLELSQYVGECLGI
                     SARTLAGAESWSSVAGLAGTTKQAAWGRWHH"
     gene            complement(3713..4828)
                     /locus_tag="VFC01_24400"
     CDS             complement(3713..4828)
                     /locus_tag="VFC01_24400"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HZN82791.1"
                     /translation="MTALLAPAAAPPLPSPSARWQEWLTYGLRWASVVERAPVVEPVK
                     TTSRTLTLHAYREAEPGPRWRALYDATWPAYRRWYIRDGLAARPSLDECRAALSQYLP
                     ELTPTWERLCRLSGDDSVAAQMLSMWGLPAFAVGCSQVVIPGEQPILVRNYDYDQSLF
                     EAVVASTNYSGHRRVLGTSDLLWGLLDGMNEDGLATSLTFGGRPGGGEGFGIPIVLRY
                     VLETCATVEQAVSALRRIPVAQSYNVALVDTIGNHATVFVAPQQRAVVSRLDATTNHR
                     LDLVEHPAHAARFNSVGRLTRLDQLRADDTSGEQLVAAMLQPPLRNSQFEIGFGTLYT
                     AKYEPAAGTATWHWPEMSWTRRFDDPDGVRTVNLAHP"
     gene            complement(4892..5041)
                     /locus_tag="VFC01_24405"
                     /pseudo
     CDS             complement(4892..5041)
                     /locus_tag="VFC01_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394185.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            5047..5715
                     /locus_tag="VFC01_24410"
     CDS             5047..5715
                     /locus_tag="VFC01_24410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82792.1"
                     /translation="MRRGLHARVADALVGGVGGEPDWRLVAAHYEQAERFDEAASAYQ
                     QASATARRRGALAEARAYLTQALSGLEHCTPGPDRDRREMASRSERGFLATAAEGYQS
                     RAAAADFERCLQLGGTDLHDDELFATLTALYGYYLARADLQRAAQVLEVLRASLEQGR
                     QWFRPVSEGRLGVVAWLRGQFAAADSHLKAATAGAAAADQQQITRCGCNLPSRSCRRT
                     STWP"
     gene            5688..6461
                     /locus_tag="VFC01_24415"
     CDS             5688..6461
                     /locus_tag="VFC01_24415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82793.1"
                     /translation="MSAHLYLALTRGVRGDLTGAEAELAQAARRAEQLSFPEGPFSLA
                     YTRFGEIWMRTEAGQLDRAAVLAADLIEQAERYGFDLWRLWGATQQATVGALAALRAD
                     KLDPTALAAHIATMTPLLDTSHTLELNIFIPSYHAVLGQLLIGAGQPERARRSLDTTL
                     QLAQDTGTCFYDAELLRLRAYTHTDPDARRADVGAALALARRQGATLFELRAALDDFE
                     LRGEPARETLVDVIGRMPADSAWPELARAPGGLSEQRWI"
     gene            6508..7728
                     /locus_tag="VFC01_24420"
                     /pseudo
     CDS             6508..7728
                     /locus_tag="VFC01_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007053875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            7950..>8409
                     /locus_tag="VFC01_24425"
     CDS             7950..>8409
                     /locus_tag="VFC01_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN82794.1"
                     /translation="MDDGRGAVTAAGLLCGSCGTELPPDSRFCNRCGAPITPAATAAE
                     YKQVTVLFADVVHSMDIAAAVGAERLRDIMAELVDRAAVVVQHFGGTVGSFTGDEIMA
                     VFGAPITLEDHAVRACLAASGIQEEAKRLASDVRERNGVELQLRVGLNSGQ"
BASE COUNT         1485 a         2723 c         2770 g         1431 t
ORIGIN      
        1 ccagccgtgt cttcgaatac gaaccgcgtg caccgctcga tccgatgagc accgacgact
       61 tgtagatccc cccgctgggg gcacgcgcat cgacgttgac caacaggtcc gctccgatcc
      121 gtcgtcgcaa ttcgacgagc cgggccatgg cgtcggggtg gctccccaga tcgtccccca
      181 cgctgctctc accccagaca acgaggtcga gctgctgacc tgccagcgtt tcggtgagcg
      241 cttcaccggc ggcttgacgc gctgtcgcat cttcgatgtc gcccggctga acgagcgcga
      301 tcctgactga cgagccttcc gcaggcgccg gtccccacca gaaccacgca gggctgacga
      361 ttgcgagggc gatcggcaac gccaccagcc acgcggtgcg caccaggagc gctcccacga
      421 ccgcggtgtt gaccgcgaca atcagaaagc tgacaagcca cacgccaccc acggacgccg
      481 gtgccagcgt cgcaggttga ttccactgcg aggcgccaag cagcgtccag ggtccgccga
      541 gtttgtccca cgatcgcgcc gcctccgcga gaacccacca gctcggcagg acgacgaccg
      601 cggcgacgac gcgccagatg gtgagctccc cggacagcag acggtgcgca gcccagcccc
      661 acggcagcca cagtgtcccg aggccgacgg cgagaacgat cagcagtggg ccggcggccg
      721 gccacagcca gtactgagtg atcagcacga agccggtcat gccggcccac gcgcggaccg
      781 cgccctgcat ggcggtcggc gcggcgcgca cgacgagcag caacggcacc agcccaaccc
      841 aggccaacca ccaccacgac aaggcgggaa aggccagagc aggcggcgcg ccggcgatca
      901 gcgccgccgc gttgccccga aagaggtcag gtagccgatg ctttggcgat gcttgcgatc
      961 gcctggtcca aggtcgcgtt gaattcctcg tcgctctgct gggcggtcag cccctcggta
     1021 agcgcccgcg agaagctcgc gatcacaccg gtgttgcggg ccagcttctc acacgcctcg
     1081 tcgcggctgt agccaccgga taatgccacc acccgaagca ctttcggatg atcgacgagt
     1141 tgcttgtaga ggttgtcggt gtcgggcagc gtcagtttca gcatgacttt ctggtcgtcg
     1201 ccgagcgcgt tgacgccgtc gagcagcgcg gcgttcagtt ggtcttcggc ctccgcctta
     1261 cgtgggctct tgatgtcgac ctcgggctcg atgatcggca ccagaccctt ggcgaggacc
     1321 tgctggccca cctcgaactg ctgcgcgacg accgcgtcca ggcctgcgcc cggcagcttg
     1381 atgaccgaac gttccttggt gccgaacacg ccattggcga ccgcgcggtc cagcaggtcg
     1441 tccaggccgg gcatcggctt catcgtctgc gcgccgtcct gctcgtcggc caggcctttg
     1501 tcgatcttga ggaaagggac gatgttcttg acgttccaca ggtagtcggc ggtcggacgg
     1561 ccttcgatct ggcggtccat cgtcatctcg aacaggatgg cgcccatgat gcggtcgccg
     1621 ttgaaggccg ggctggtgat gatgcgggta cgcatctcgt gcaccaggtc gaacatctgc
     1681 tcgtcgccgg agtaggcgtc ctcggggatg ccgtagagct tcagcgcctt gggcgtgctg
     1741 ccgccgcttt gatcgagcgc ggcgatgaag ccggctccgt tcttggcctt gttgaactgg
     1801 tcgttgttca tttccgccct ttctccttca ccaagcaatg tactaatgcc acactcaggc
     1861 tgacgaagag gtcagtcatg acagcaacgt tcagcaagac tcctatagcg ccccgcagga
     1921 acggcgcgcc ccctcccgac gtcccactcg ctgatatcga cctggggact gtggagtttt
     1981 ggaatttgga tgacgacctg cgcgacggcg catttgcgac gctgcggcgc gagtcaccga
     2041 tcaccttctt tgaggttccc gaggtcgcgg gtttcgcccc gggcgccggg cactgggcct
     2101 tgacgaccta cgacgacgtt catcacgcga gccgccaccc cgagatcttc agttcgatcc
     2161 cgacgagcac cgcgctcaat gacgtgcccg ccgagatcgc agaatttgcc ggttcgatga
     2221 tcagcctgga cgatccccgt caccttcggc tgcgcaagat cgtcaaccgc gcgttcacac
     2281 ccaaggtggt tggccgaatc gaggacagcg tgcgtgaccg tgcgcgcagg cttgtgactg
     2341 acatgatcac caaccatccc gacgcgcacg ccgacttcgt tgccgaggtg tctggtcccc
     2401 tgccgctgca gatcatctgc gacatgatgg gcatcccgga ggaggacgag gagaacgtct
     2461 tccactggac gaatgtgatg ctgggcgtcg gagatgacga agcgtccggt gagttcgatg
     2521 aggtcatcaa gacagtcacc gagatcgccg aatacggcgt cgcgttggcc gaggaacggc
     2581 gggcccggcc cggcgacgac ctgaccacga acctggtgca ggccgacgtc gacggcgagc
     2641 ggctgacgtc ggtggagatc gcgtcgttct tcattctgtt gtcggcggcc ggcaacgaga
     2701 cgacgcgaaa tgcgatcagc cacggcatgg ttgcgctcac ccgttatccc gagcagcggg
     2761 agacctggtg gtccgatttc gacggtttcg ccgccacggc cgtcgaggag atcgttcggt
     2821 gggcttcccc ggttatcttc atgcggcgca acctcactga ggacatcgag atgggcggca
     2881 tcccgatgaa ggccggtgac aaagtctcga tgtggtacaa ctctgccaac cgtgatgagc
     2941 gaaagttcac caatccgtgg ctgtttgact tgacgcgcga cccgaatccg catgtcggtt
     3001 ttggtgccgg cggcgctcat ttctgcttgg gtgccaacct ggcccgtcgt gagatacggg
     3061 tgctgttctc cgagctctac cgacagattc ccgatgtcgt cgccgtccag gaaccggcga
     3121 tactgcactc ggcgttcatc cacggcatca aacgactgcc cgtcgagtgg acaccgccga
     3181 actgagcaga tctaccaccc gcgctgtttc cactcactca actgcgggcg ttccgccccg
     3241 agcgtggtgt cgtcaccgtg ccccgggtac acgacagtcg tatccgcgta gaggttaaac
     3301 acccggctag tgacatcggt gagcagttgc gtgaaatcgc ctgtcttcca ggtttttccg
     3361 acgccgccgg ggaacagcga aggtcgtaag taggtaacat acgtggaggc ccggaaggcg
     3421 gtctctatgc cggatgaaga catcacgtca accattctgc gcgcagcgat ctacgcccta
     3481 tccgcggctt agtggtgcca ccgcccccag gcggcctgtt tggtggtgcc ggcgagaccg
     3541 gcaacactgc tccaggactc ggctccagcc agagtgcggg ccgaaatgcc caggcattcg
     3601 ccgacgtatt gactgagttc gagcaaggtt tgaaaggcgg ccgggtcggt gctgttggcg
     3661 agcgcatcca gcagggtgcg ggcttcggcc gcaatatcgg ttggttcccg gctcatgggt
     3721 gggccaaatt gactgtgcgt acgccgtccg ggtcgtcgaa gcgtcgcgtc caggacatct
     3781 cgggccaatg ccacgttgca gttccggctg caggttcata cttggccgtg tagagagtgc
     3841 cgaagccgat ctcgaactgg ctgttgcgca acggtggctg cagcatcgcg gcgacgagtt
     3901 gctctcccga cgtatcgtcg gcgcgcaact ggtccaaccg cgtaagtcgc ccgacgctgt
     3961 tgaatctagc ggcgtgggcg gggtgttcga ccagatcgag tcggtggtta gtcgtcgcgt
     4021 cgagtcggct cactacggca cgctgctgcg gggcgacaaa aaccgtggcg tgattaccaa
     4081 ttgtgtcgac cagcgccacg ttgtaggact gcgcaacagg aatccgccgg agtgcactga
     4141 ccgcctgttc gacggtggca caggtctcta gcacgtagcg cagcacgatg gggatcccaa
     4201 agccttcgcc accgccgggt cggccaccga acgtcagcga ggtcgccagc ccgtcctcgt
     4261 tcatcccgtc cagcagaccc cagagcaggt cgctcgtccc gagcacccgg cgatgcccgg
     4321 agtagttggt ggaagcgacg acggcttcaa agagcgactg gtcgtagtcg tagttgcgga
     4381 ccaagatcgg ctgctcacca ggtatgacaa cttgggaaca accgacagcg aaagccggaa
     4441 gtccccacat cgacagcatc tgcgcggcaa cagagtcgtc accactcagg cgacacagcc
     4501 gttcccaggt aggcgtcagc tcggggaggt attgggaaag tgcggcccga cattcgtcaa
     4561 gggaggggcg ggcggccagc ccatcccgta tgtaccagcg gcggtacgcc ggccaggttg
     4621 cgtcgtacag cgctcgccag cggggacccg gctctgcctc tcggtacgcg tggagcgtca
     4681 aggtgcggct cgtggtcttg acgggctcga ctaccggggc gcgctcgacc accgaggccc
     4741 agcgcagtcc gtaggtgagc cactcctgcc agcgagcact aggtgagggg aggggtgggg
     4801 ctgcggctgg agccaagagt gctgtcatgc gtcaagatta tcttgacgat cccttcatgc
     4861 gtcaagcttt acttgacgga atgcttgcgg gtcaccaacc ccgcgcacgc cattcgctaa
     4921 ggtgcggacg ttcggtcccg agcgtcgtgt cgtcgccgtg cccggggtag atcactgtcg
     4981 tgtccgggta gacgtcgaac acccggctag tgacatcggt gagcagttgc gtgaaatcgc
     5041 ctgagtgtgc gtcgaggtct gcacgcccgg gtggccgatg cgctggttgg cggtgtgggc
     5101 ggggagccgg actggcggct ggtcgctgcc cactacgagc aggccgagcg gttcgacgag
     5161 gcggcctcgg cctaccaaca ggcatccgcg actgcccgcc gccgaggcgc gctggccgag
     5221 gcccgcgcct acctgaccca ggccctctcc gggctcgagc actgcacacc cggcccggat
     5281 cgcgaccggc gcgagatggc ctcgcgctcg gagcgaggat ttctcgctac ggccgcagag
     5341 ggctatcaaa gccgcgccgc cgccgcggac ttcgagcggt gcctgcagct gggcgggacc
     5401 gacctgcacg acgacgagct gttcgcgacg ctgaccgccc tgtatggcta ctacctcgcc
     5461 cgtgccgacc tgcaaagggc agcccaggta cttgaggtcc tgcgcgcgag cctcgagcag
     5521 gggcggcagt ggttccggcc ggtgagcgag ggcaggttgg gagtggtggc gtggctgcgc
     5581 ggccaattcg ctgccgccga ctcccatctc aaggcggcca ccgcgggtgc ggctgcagca
     5641 gaccagcaac agattacgcg gtgtggttgc aacctaccga gccgatcgtg tcggcgcacc
     5701 tctacctggc cttgacgcgc ggcgtgcgcg gcgatctcac cggcgccgag gccgagctgg
     5761 cccaggcggc acgccgggcc gaacagctca gctttccgga gggcccgttc agcctcgcct
     5821 acacgcgttt cggggagatc tggatgcgca ccgaagctgg ccagctggac cgtgccgcgg
     5881 tactcgccgc cgacctgatc gagcaggccg agcggtacgg cttcgaccta tggcggctgt
     5941 ggggggcgac ccagcaggcc actgtcggcg ccctggccgc actccgcgcc gacaaactcg
     6001 acccgaccgc gctggcggcc cacatcgcga ccatgacacc gctcctcgat accagccaca
     6061 cgctcgagct gaacatcttc atcccctcct accacgccgt cctcgggcag cttttgatcg
     6121 gcgcaggcca acccgagcgg gcccgccgca gcctggacac cacactgcag ctggctcagg
     6181 acaccgggac gtgcttctac gacgccgaac tgttgcggct tcgcgcctac acccacaccg
     6241 acccggacgc tcgccgagca gacgtcgggg ccgctctcgc actcgcccgc cgccagggcg
     6301 cgaccctgtt cgagctgcgc gccgcgctcg acgatttcga actacgcggc gagcctgcgc
     6361 gcgaaactct ggtcgatgtc attggccgca tgccagccga cagcgcctgg ccggaactgg
     6421 cgcgggcccc agggggactg tccgaacaac ggtggatctg atcttggtct gacacgccgg
     6481 gcgtgagctt ggcgggaagg actgacgatg tcagaaacac tcgatcctgt ggcgatggaa
     6541 ttggatcagc ggcggctggc tgaacagctg ctggcgcagg cgaaggaaca gggcgttgag
     6601 ctggtcggcc cgaacgggct cctcaaccag ttgaccaaga acgtgctcga gaccgcgctg
     6661 gatgcggaga tgaccgagca cctgggctat gacaagcacg acccggccgg gcgcggcagc
     6721 ggtaactccc gcaacggcac ccgagccaag acggtgctca ccgagatcgg gcccgtcgag
     6781 atcgaagtgc cccgtgacac caacagctcg ttcgagccgc agctcgtcaa gaagcgtcag
     6841 cgccggttga cgggtatcga cgagatcgtc ttgtcgctca cggcaaaggg attgacgact
     6901 ggggaggtcg ccgcccactt cgccgacgtc tacggcgcca cggtctccaa ggacaccatc
     6961 tcccgcatca ccgacaaggt gatcagcgag atgaccgact ggtgcaaccg gcccctggag
     7021 ccggtgtatc cggtggtgtt catcgacgcg atccacgtca agatccgcga cggccaggtc
     7081 accaaccgac cgatctatgt ggcggtcggg gtcacctgcg ccggggagcg tgacatcctc
     7141 gggctgtggg ccggcgacgg cggcgagggg gctaagttct ggctggcggt gctgaccgag
     7201 atcaagaacc gcggcaccgc cgatgtgtgc atcgtggtct gcgacgggct gaaggggcta
     7261 cccgaggcgg tcaacacggt gtgggatcgc gcggtcatcc agacatgcgt gatccacctg
     7321 ttacgcaaca cctttcgcta cgcatcacgc aagtactggg atcagatcgc caaggatatc
     7381 cggccggtgt acaccgcccc gactgaggcc gctgccaagg aacggtttgt cgaattcacc
     7441 gccaaatggg gtgctcagta tccggcgatc atccgactgt gggagcacgc ctggagcgag
     7501 ttcgtgccgt tcctggacta cgacgtggag atccgccggg tcatctgctc gacgaatgcc
     7561 gtggagtcga tcaacgcccg ctaccggcgc gcgatccgcg cgcgcgggca tttcccgacc
     7621 gaacaatccg ccctgaagtg tctgtacctg gtgactcgat cactggatcc gaccggccgc
     7681 ggtaaggcac gatgggcaat gaggtggaag ccagcactta acgcgtttgg atcaccttca
     7741 acggccggat caccccgacc ggcaactaac caaatgccaa ggtcggatcc accgttaatc
     7801 ggacagcccc gccccaggcc gcattggata gctgactgac cgccaaccga ccgtgtctcg
     7861 atagtgacga catatgaggg ccgacttcgt gtcggaggtg gcacggcgat atgacggaca
     7921 tcgttgacca gcggtgacac gatactgacg tggacgacgg tcgcggcgcg gtgacagcag
     7981 cgggactgtt gtgtgggtcg tgcggcacgg agctgcctcc ggactcaagg ttctgcaaca
     8041 ggtgcggcgc gccgatcacg ccagccgcga cggcggcgga gtacaagcag gtgacggtgc
     8101 tgttcgccga tgttgttcat tcgatggaca tcgcagcggc ggtcggtgcg gagcggctgc
     8161 gcgacatcat ggccgagctg gttgatcggg ctgccgtggt ggtgcagcac tttggcggca
     8221 cggtcggcag cttcaccggc gacgagatca tggcggtgtt cggcgcacca atcacgctgg
     8281 aggaccacgc tgtccgcgcc tgcctggcgg cgtcgggcat acaagaggag gcgaagcggc
     8341 ttgcctccga cgtccgagag cggaacggtg tggagctgca gttgcgggtg ggcctgaact
     8401 cgggccagg
//
LOCUS       DASRJA010000552        11485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2345051,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000552 DASRJA010000000
VERSION     DASRJA010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11485
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2345051"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VFC01_24430"
     CDS             complement(<1..1134)
                     /locus_tag="VFC01_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82795.1"
                     /translation="MAALSAVVFSLSWWLGLYLLARDPRKPVLVLAAIGLTSFASVVA
                     LDAVRVLSGSDALSSVEVYLVTVPGIAWFAVLLEMSRPSDTWRSRAGEVALVAAVAAV
                     ALFGAAMAGNVDGPLRMGHWVMFGAISLSSLGAMLRAVFGPWQPRPVVGYVVVATLFF
                     ALGNAILIIPLGLVPSWLALASTGCDVALLGVAVAMWDAFDEGQALRADMLRSFAATA
                     VVALLFGGQLLIGLAVTGQETALTVLLFTSLGIAIAINVLADPLAGLLDRVTFSRSPA
                     LRADRAALRDAEAALPRRSTSPLDGVDDKTFARLTRRALGHYGDLSKLVASPLTALPT
                     IDARLAARGAPDQPLERANELKALLADHIARLKPRDGGEFGTTE"
     gene            complement(1150..1941)
                     /locus_tag="VFC01_24435"
     CDS             complement(1150..1941)
                     /locus_tag="VFC01_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HZN82796.1"
                     /translation="MDIIPPRLKEPMYRLYEMRLRQGLASSKSELPRHIAVLCDGNRR
                     WARDAGHDDVSYGYRMGARKIAEMLRWCQEAGIEMATVYLLSTENLQRDAGELASLIE
                     IITDVTEEICAPANRWSVRTVGDLELIGEEPARRLREAVESTNGNGSGFHVNLAVGYG
                     GRQEIVDAVRALLGKELADGATGDQLIEAVSIDAISENLYTSGQPDPDLVIRTSGEQR
                     LSGFLLWQSAYSEMWFTEAYWPEFRRVDFLRALRDYTARHRRYGM"
     gene            1997..2734
                     /locus_tag="VFC01_24440"
     CDS             1997..2734
                     /locus_tag="VFC01_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HZN82797.1"
                     /translation="MAQIDTADEAQQQPPRVYAEDFPEAVVDGVAHFIGKPRLRGWIH
                     VYAAIVACIAGAALVSVSWSLQSTRAGLATLLYTLTIVAMFTVSGTYHRVTWKSASAR
                     KWMKRADHSMIFIFIAGSYTPFALLALPERSGWVLFWIVWGGAIAGVLLKMFWPSAPR
                     WFGVPLYILLGWVAAWFVGPIMHGAGVAALVLLIVGGALYSIGGVLYALKWPNPWPTT
                     FGHHEFFHACTAVAAICHYIAMWFAVF"
     gene            complement(2789..3148)
                     /locus_tag="VFC01_24445"
     CDS             complement(2789..3148)
                     /locus_tag="VFC01_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN82798.1"
                     /translation="MPSAEAITNTVNRYLETVSQGRPDDIAALYADDATVEDPVGSEV
                     HIGRQAIRGFYKAVEGANATTEIVTLRALGNEAAFFWKLSIAGMTIEIISTMTFDDEG
                     KIASMKAYWGPDNITQG"
     gene            complement(3159..5135)
                     /locus_tag="VFC01_24450"
     CDS             complement(3159..5135)
                     /locus_tag="VFC01_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZN82799.1"
                     /translation="MANQLGTATSPYLRQHADNPVHWQQWTPEALAEAATRDVPILLS
                     VGYAACHWCHVMAHESFEDDQVAAVMNEGFVNIKVDREERPDLDAVYMNATVALTGQG
                     GWPMTCFLTPDGRPFFCGTYYPKPNFLQLLAAVTETWRTRRGEVEEASDRIAGELRSM
                     VSGLPGGGPPVQPALCDHAVAAALQDEDVERGGFGGAPKFPPSALLEALLRSHERTGD
                     ILPLETVERTCTAMARGGIYDQLAGGFARYSVDASWVVPHFEKMLYDNALLLRVYAHW
                     ARRTSNPLARKVTDETARFIIDGLGADGMFTSSLDADADGREGLTYAWTPAQLREVLG
                     DDDGRWAASVFNVTEAGTFEHGSSVLQLPADPDDAVRFERVRTALLAARLTRPQPGRD
                     DKVVTAWNGLAITALTEASVALDRPEFLDAAMQCARSIVNLHIVDGRLRRASLGGRVG
                     DSAAILEDHATLATGLLALYQLTGDASWLDAAMGLVDIALDHFADPEREGRWFDSADD
                     AEQLVVRPADPLDGATPSGASSIAEALQLAAHLAPQADRYAAAADATLASATPILARL
                     ARSGGHWLAVAETAVRGPIQIAVACDPQHSELLSAARKLAPGGAIVVGGPVNSSELLL
                     DRDRVNGSDAAYVCRGRVCDLPLTTIDELTAALR"
     gene            complement(5160..5879)
                     /locus_tag="VFC01_24455"
     CDS             complement(5160..5879)
                     /locus_tag="VFC01_24455"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HZN82800.1"
                     /translation="MTRLIPVPLPDSGAVRVHPAPEALPTLRHEGPGRNRFDDPRGIF
                     LMRYTAESLRGCLVETMARFRPNTSAEALLAAVQFVAPEDEPELPTVWAVNDWLAEQR
                     VGLVTVVTPQPLLVDIEAAELLVDLDAHPLVREAIDNSPLSDGDSPVHLDAGLIRLGG
                     DVGRPVTQAVSRAVHEQHPEVDGIAYWSRIDSSERCWAIYGHVPVDVVITDLSVDDPQ
                     HRAAVQDVAALLKVPLPPAWR"
     gene            complement(5876..6145)
                     /locus_tag="VFC01_24460"
     CDS             complement(5876..6145)
                     /locus_tag="VFC01_24460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82801.1"
                     /translation="MGLKDARTVRNWVEKQAEPREHDVVSRLAVLFRVVRAIESIYSP
                     TVAAAFLRSSNPQLDDEAPLVVLATQPVDEAEQALLAAARAFLEG"
     gene            complement(6454..6687)
                     /locus_tag="VFC01_24465"
     CDS             complement(6454..6687)
                     /locus_tag="VFC01_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82802.1"
                     /translation="MNEFLVLAVGLLADDAPRNTGPDFGKASPFGLVIVVLLLIGTFA
                     LVWSMNRHLRKLPKSFDSEPEQPTEDPKRESGG"
     gene            complement(6684..7550)
                     /gene="mca"
                     /locus_tag="VFC01_24470"
     CDS             complement(6684..7550)
                     /gene="mca"
                     /locus_tag="VFC01_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HZN82803.1"
                     /translation="MSELRLMAVHAHPDDESSKGAATMARYVDEGVRVMVVTLTGGER
                     GDILNPAMDLPEVHGRMAEIRRDEMAKAAEILGVEHHWLGFIDSGLPEGDPPPPLPDG
                     CLGLVPLEEPVEELVRVIRKFKPHVMTTYDENGGYPHPDHIRTHQVSVGAYEAAGDYR
                     MFPDAGEPWDVLKLYYNHGFLRKRMQLLQDEFAKHGQEGPFEKWLKHWDPEHDVFANR
                     VTTRVECAKYFAQRDDALRAHATQIDPLGDFFRAPIEWQQRLWPTEEYELARSRVPVA
                     FPETDLFTGVEP"
     gene            7661..8083
                     /locus_tag="VFC01_24475"
     CDS             7661..8083
                     /locus_tag="VFC01_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4307 domain-containing protein"
                     /protein_id="HZN82804.1"
                     /translation="MIERPADRYGRQRLSRGNRRWIAIGLGVLVLVVGIVIAVIASQR
                     LGSGDVKGELGGYQLVDDETVQVTITVTRDDPSRPVVCIVRARSIDGSETGRREVLVA
                     PSTQKTVQVTTVVKASRPPVVGDVYGCGTDVPSYLVAS"
     gene            8323..8817
                     /gene="greA"
                     /locus_tag="VFC01_24480"
     CDS             8323..8817
                     /gene="greA"
                     /locus_tag="VFC01_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZN82805.1"
                     /translation="MTDTQVTWLTQEAYDRLKAELDQLIANRPVIAAEINDRREEGDL
                     RENGGYHAAREEQGQQEARIRQLQELLNNAKVGEAPKQSGVALPGSVVKVYYDDNKSD
                     TETFLIATRQEGVSDGKLEVYSPNSPLGGALIDAKVGETRTYQVPNGNTVKVTLVSAE
                     PYHS"
     gene            8881..9543
                     /locus_tag="VFC01_24485"
     CDS             8881..9543
                     /locus_tag="VFC01_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HZN82806.1"
                     /translation="MAQIAEDLFLLLLDNASAQPGLDRVRRQRVLSAAVLLDLAHACR
                     IRPAMAGEPVEPGRLVALAVPGPMDPVVAPAFQLLQRRPLRPATAVKRLSKHTEENLA
                     NYLEYMGQIRRVRLNSKRFSHPYAWPLTNRDRVGPARSALLGALFDRRPPTPTTAAII
                     SLLHAVDGLGALLSLNDRGWRWVHARAGEIAMGSWVDEYPTAMPEVNLAITASAVRGA
                     LA"
     gene            complement(9535..10689)
                     /locus_tag="VFC01_24490"
     CDS             complement(9535..10689)
                     /locus_tag="VFC01_24490"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HZN82807.1"
                     /translation="MTEQQRFQGLATKAIHAGFRPDPGTGAVNAPIYASSTFAQDGVG
                     GLRGGFEYARTGNPTRAALEASLAAVEDAAFGRAFASGMAATDCVLRAVLRPGDHVVM
                     PDDAYGGTFRLIDKVFTQWGIDYTAVALSDVDAVRAAITPKTRLIWLETPTNPLLSIA
                     DIAAISQLAGPSNIKVLVDNTFASPALQQPLLLGADIVLHSTTKYIGGHSDVVGGALL
                     TNDEELDTAFAFLQNGAGAVPGPFDAYLTMRGLKTLVLRMQKHSENGGAVAEFLAGHP
                     AIDTVLYPGLPSHPGHEVAKRQMSAFGGMVSVRMRGGPQAAHDFCSRTEVFILAESLG
                     GVESLIEYPGAMTHASTAGSQLEVPDDLVRLSVGIEDAADLLADLEQALG"
     gene            10745..>11485
                     /locus_tag="VFC01_24495"
     CDS             10745..>11485
                     /locus_tag="VFC01_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN82808.1"
                     /translation="MATRLEDLLDLDSMLTPEDIELRQTVRRFGEQRLRPHIADWFEA
                     GEVPVRELAADLGKLGVLGMHLKGYGCGGSTATAYGLVCQELEAVDSGLRSLVSVQGS
                     LAMFAIHRLGSEEQRKQWLPAMATGDVIGCFGLTEPDFGSNPGGMRTTARRDGSDWIL
                     NGSKMWITNGSVADVAIVWARSKEGIVGFAVPAGTPGFSARDMTHKLSLRASVTSELH
                     LDDVRLPADAQLPDANGLSGPLSCLSEAR"
BASE COUNT         1945 a         3856 c         3795 g         1889 t
ORIGIN      
        1 ttcggtcgtg ccgaactcgc caccgtcgcg cggtttgagg cgggcgatgt gatcggcgag
       61 cagagccttc agttcgttgg ctcgctccag cggctgatcg ggcgcgccgc gcgcggcgag
      121 ccgcgcatcg atcgtcggca gcgcggtgag cgggctggcg acgagcttgg acaagtcgcc
      181 gtagtgaccg agcgcgcggc gggtcagtcg ggcgaaggtc ttgtcgtcga cgccgtcaag
      241 cggactggtc gaccgcctcg gcagcgcggc ctccgcatcc cgcagcgcgg cacggtcggc
      301 ccgcagcgcg ggcgagcgcg agaacgtcac ccggtcgagc agcccggcca gcgggtcggc
      361 gagcacgttg atcgcgatcg cgatgccaag gctggtgaac agcagcaccg tcagcgctgt
      421 ctcctgaccg gtcactgcca ggccgatcaa cagctgaccg ccgaacagca gtgccaccac
      481 ggccgtcgcc gcgaacgatc gcaacatgtc ggctcgcagc gcctggcctt cgtcgaacgc
      541 atcccacatc gccacggcca ccccgagcaa tgcgacgtcg catccggtgg acgccaatgc
      601 caaccagctc ggcaccagac cgagcgggat gatcaggatc gcgttgccca gcgcgaagaa
      661 cagcgtggcg accacgacgt agccgacgac gggtctcggc tgccacggac cgaatacggc
      721 tcgcagcatc gcgcccaatg aagacagcga gatcgcgccg aacatcaccc agtggcccat
      781 ccgcagcggg ccgtcgacgt tgcccgccat tgccgcgccg aacagtgcga ccgccgcgac
      841 tgcggcgacg agcgcgacct cgcccgcccg gctgcgccag gtgtcgctcg gccgcgacat
      901 ctcgagcagt acggcgaacc aggcgatgcc gggcacggtg accagataga cctcgacgct
      961 gctcagcgcg tccgaaccgc tcagcacgcg gaccgcgtcc agcgccacca ccgacgcgaa
     1021 actggtcagc ccgatcgcgg ccaggaccag tacgggtttg cgtgggtcac gggccagcag
     1081 gtacaggccc aaccaccagc tgagcgagaa gaccaccgct gacagcgcag ccatagtcct
     1141 agtttggcac tacatgccgt aacgacggtg gcgcgcggtg taatcgcgca gcgcgcgcag
     1201 gaagtcgacc cgccggaact ccggccagta ggcctccgtg aaccacatct ccgagtaggc
     1261 gctctgccac agcaggaagc cggacagccg ctgctccccc gacgtgcgga tcactagatc
     1321 ggggtcgggt tggccggagg tgtaaaggtt ttccgagatc gcgtcgatgc tgacggcctc
     1381 gatcaactgg tcgccggtcg cgccgtcggc cagttccttg ccgagcagcg cccggaccgc
     1441 gtcgacgatc tcctgtcgtc cgccgtaacc cacggcgagg ttgacgtgaa agccgctgcc
     1501 gttgccgttg gtcgactcga cggcctcgcg caggcgccgg gccggttcct cgccgatgag
     1561 ttctaggtca ccaaccgtgc gcacgctcca ccggttggcg ggtgcgcaaa tctcctcggt
     1621 gacgtcggtg atgatctcga tgagcgacgc cagctcgccc gcgtcacgct gcaggttttc
     1681 ggtggacagc aggtagacgg tcgccatttc gatgccggcc tcctggcacc agcgcagcat
     1741 ctcggcgatc ttgcgggcac ccatccggta gccgtagctg acgtcatcgt gaccggcatc
     1801 acgtgcccac cgtcggttac cgtcgcatag caccgcgatg tggcggggca gttcagattt
     1861 cgaggacgcc agtccctgac gtagccgcat ctcgtagagg cggtacatcg gctccttgag
     1921 tcgaggagga atgatgtcca cggagatcca gactactgtg acggtcggaa gtccccatgc
     1981 cattgatgga ggtgacatgg cccagatcga taccgccgac gaagcccagc aacagccgcc
     2041 gcgcgtgtac gccgaagact tccccgaagc cgtcgtcgac ggcgtcgccc atttcatcgg
     2101 aaaaccccgg ctccgcggct ggatccacgt ctacgcagcg atcgtggcct gtatcgccgg
     2161 cgcggcgctg gtgtcggtct cgtggtcgct gcaatcgacc cgcgccgggc tggccacgct
     2221 gctgtacacc ttgacgatcg tcgcgatgtt cacggtcagc ggcacctatc accgggtcac
     2281 ttggaagtcg gcgtccgccc gcaagtggat gaaacgggcc gaccactcga tgatcttcat
     2341 cttcatcgcg ggcagctaca cgccgttcgc cctgctggcg ctgcccgagc gcagcggttg
     2401 ggtgctgttc tggatcgtct ggggcggggc gatcgccggg gtgctgctga agatgttctg
     2461 gccgtcggcg ccgcgctggt tcggtgtgcc gctctacatc ctgctcggct gggtggctgc
     2521 ctggttcgtc ggaccgatca tgcacggcgc gggcgtcgcc gcactggtgt tgttgatcgt
     2581 cggcggcgcg ctgtattcga tcggcggcgt gctgtacgcg ctgaaatggc cgaacccgtg
     2641 gccgacgacg ttcggccatc atgagttctt ccacgcgtgt accgcggtcg cggcgatctg
     2701 ccactacatc gcgatgtggt tcgccgtctt ctagcgattc ggcgcgctta tcaacgccca
     2761 ccagcgacgg ttagcgcgcc gaaatcgctc agccctgggt gatgttgtcc gggccccagt
     2821 acgccttcat cgacgcgatc ttgccctcgt cgtcgaacgt catggtgctg atgatctcga
     2881 tggtcatgcc agcgatgctg agcttccaga agaaggccgc ctcgttaccg agcgcgcgca
     2941 gcgtcacgat ctcagtcgtg gcgttggcgc cctcgactgc cttgtagaac ccgcggatcg
     3001 cctggcgccc gatatgcacc tcactgccga cgggatcctc gacggtggcg tcgtcggcat
     3061 agagcgcggc gatgtcgtcg ggccggcctt gcgacacggt ctccagataa cggttgacgg
     3121 tgttggtgat ggcttcagcg ctgggcatgg cggcgacgct acctcagcgc cgcggtcaat
     3181 tcgtcaatag tggtgagggg caggtcgcac acgcggccgc ggcagacgta ggcggcgtcg
     3241 gacccgttga cccggtcacg gtcgagcagc agctccgagg agttgaccgg accgcccacg
     3301 acaattgcac cgccgggcgc caatttgcgt gcagcggaaa gtaattcaga atgctgagga
     3361 tcgcaggcga cggcgatctg gatgggtccg cgcaccgcgg tttcggcgac cgcgagccag
     3421 tggccacccg agcgcgcgag ccgggcgagg atgggcgtag cgctggccaa ggtcgcgtcg
     3481 gcggcggcgg cgtaacggtc cgcttgtggc gccaagtggg cggccagctg caacgcctcg
     3541 gcgatcgacg aggcgccgga cggggtggcg ccgtcgaggg ggtcggcggg ccggaccacc
     3601 agctgttcgg cgtcgtcggc gctgtcgaac cagcggccct cccgttccgg gtcggcgaaa
     3661 tggtccagcg cgatgtcgac cagccccatg gccgcgtcca accacgacgc gtcgcccgtc
     3721 agctgataca gcgccaacag gccggtcgcc agggttgcgt ggtcctcaag gatcgccgcg
     3781 ctgtcaccca cccggccgcc gaggctggca cgccgcagcc ggccgtccac gatgtgcagg
     3841 ttgacgatcg aacgcgcgca ttgcattgca gcgtcaagga attcgggcct gtcaagcgcg
     3901 acactcgcct cggtgagcgc ggtgatcgcg agcccgttcc aagcggtgac gaccttgtcg
     3961 tcgcggccgg gctgcggccg cgtcagccgc gcggccagca gcgcggtgcg cacccgctcg
     4021 aaccgcacgg catcatcggg gtctgccggc aattgcagca ccgagctgcc gtgttcgaat
     4081 gtccccgcct cggtgacatt gaaaaccgaa gcagcccaac gcccgtcgtc gtcacccaac
     4141 acctcgcgca gctgcgcggg cgtccatgcg taggtcagcc cctcgcgacc gtcggcatcg
     4201 gcgtccagcg atgacgtgaa catgccgtcg gcgcccagcc cgtcgatgat aaagcgcgcc
     4261 gtctcatcag tcactttgcg cgccaacggg tttgacgtgc gacgggccca gtgcgcatac
     4321 acgcgcagca acagcgcgtt gtcgtacagc atcttctcga agtgcggcac gacccacgat
     4381 gcgtcgacgc tgtagcgggc gaaaccaccg gccagctggt cgtagatgcc gccgcgcgcc
     4441 atcgcggtgc aggtccgctc gaccgtctcc aacggcagga tgtcgccggt gcgttcgtgg
     4501 ctgcgcagca gcgcttccaa cagagccgac ggcgggaact tcggcgcgcc accgaagccg
     4561 ccgcgctcaa cgtcttcgtc ctgtaaggcc gcggctacgg cgtgatcgca caatgccggc
     4621 tgcaccggcg gcccaccgcc cgggagtccc gacaccatcg accgcaactc acccgcgatc
     4681 cggtcggaag cctcctccac ctcgccacga cgcgtgcgcc acgtctcggt gacggcggca
     4741 agcagctgca ggaaattcgg tttcgggtaa taggtgccgc aaaagaacgg ccgcccgtcg
     4801 ggggtgagga agcacgtcat cggccagccg ccctgccccg tcagcgcgac ggtcgcgttc
     4861 atgtacacgg cgtcgaggtc gggccgctct tcgcggtcga ccttgatgtt gacgaatccc
     4921 tcattcatca ccgcggcgac ctgatcgtcc tcgaacgatt cgtgggccat cacgtggcac
     4981 cagtggcacg ccgcgtatcc gacggacagc aggatcggca cgtcgcgcgt cgccgcttcg
     5041 gccagcgcct cgggcgtcca ctgctgccag tgcaccggat tgtcggcgtg ctgacgcagg
     5101 taggggctcg tcgccgtacc aagctggttg gccaccgctt cagcctaggt ggagcgtcat
     5161 tacctccaag ccggcggtag tggcactttg agcagcgccg ccacgtcttg gaccgccgca
     5221 cgatgctgcg ggtcgtccac cgacaagtct gtgatcacta catccacggg cacgtggccg
     5281 taaattgccc agcaccgctc gctggaatcg atccgagacc agtaagcaat gccgtccact
     5341 tcggggtgct gttcgtgcac tgctcgcgat acagcctgag tgacggggcg cccgacatcg
     5401 ccgcccagcc gaatgaggcc cgcgtccaga tgcacagggg agtcgccatc gctgagcggg
     5461 gagttgtcga tggcctcgcg taccagcggg tgagcatcca gatcgacaag aagctcggcg
     5521 gcctcgatat cgaccagaag cggttgcggc gtcacgacgg ttaccaagcc cactcgttgc
     5581 tcggcgagcc aatcgttgac agcccaaaca gttggcaact caggttcgtc ctctggggcg
     5641 acaaactgca cggcggcgag caacgcctcg gcgctggtgt tcggacggaa ccgcgccatc
     5701 gtctccacca ggcagccgcg aagactctcg gcggtgtaac gcatcaagaa aattccgcgc
     5761 ggatcgtcaa acctgtttcg cccaggaccc tcgtgacgca acgtcggaag cgcttcgggc
     5821 gctggatgaa cccgaaccgc gcccgaatct ggcaacggta cgggtatcaa acgggtcacc
     5881 cttcgaggaa ggctcttgct gccgcgagca gcgcctgctc ggcttcgtcg acgggttggg
     5941 tggcaagcac tactagcggc gcttcgtcgt ccagctgagg attggacgac ctgaggaatg
     6001 ccgcagctac ggtcgggctg tagatggact cgatggctct cacaacccta aacagaacag
     6061 cgagtcgact taccacgtca tgctcgcggg gctccgcctg cttctcgacc caattgcgaa
     6121 cggtgcgggc gtctttcaat cccacggcag ccgtcgtata acgcactccc actgactcga
     6181 gtaggtgacg cgcttgctcc catgcagggg taacaatggc ttccttgtac gcggcttccc
     6241 acgcgtctgc cacagcagag gaggggtgca ccacagcgct atccatagcg tacctcctaa
     6301 cggcacctct aatgctacca ctcgcgcaaa actgcagcta gttgcaacga tatcggtgac
     6361 gggtcagtgg accggcgtcg tcctcggccg gaccacccct tctgccggcg tcaacaattg
     6421 atgacgcgct ccacgaggtg gctgccttcg aactcagccg ccggactcac gtttggggtc
     6481 ctcggtcggc tgctccggct ccgaatcgaa cgacttgggc agcttgcgca ggtgccgatt
     6541 cattgaccac accaacgcga acgtgccgat caacagcagc acgacgatga cgagcccgaa
     6601 cgggctggcc ttgccgaagt ccggcccggt gttgcgggga gcgtcgtctg cgagcagccc
     6661 gacagcgagc accagaaact cgttcacggc tctacccccg tgaacagatc ggtctcgggg
     6721 aatgccaccg gcacccgcga gcgggccaac tcgtattcct cagtgggcca tagccgttgc
     6781 tgccactcga tcggcgctcg gaagaagtcc ccgagcgggt cgatctgtgt ggcgtgcgcg
     6841 cgcaaggcgt catcgcgctg ggcgaaatac ttcgcgcact cgacgcgcgt cgtcacccgg
     6901 ttggcgaaca cgtcatgctc gggatcccaa tgcttgagcc acttttcgaa cggaccttcc
     6961 tgtccgtgtt tggcgaattc gtcctgcagc agctgcatgc gcttccgcag aaagccgtgg
     7021 ttgtagtaca gcttgagcac atcccacggc tcacccgcgt caggaaacat ccggtagtcg
     7081 ccggcggcct cgtatgcacc cacagacacc tgatgggtgc ggatgtggtc cggatgcggg
     7141 tagccgccgt tctcgtcgta ggtggtcatc acgtgcggct tgaacttgcg gatcacgcgc
     7201 accagttcct cgaccggctc ctccagcggg accaggccca ggcaaccgtc gggcagcggg
     7261 ggcggcgggt cgccctcggg caggccggag tcgatgaagc cgagccagtg gtgctcgacg
     7321 ccgaggatct cggcggcctt ggccatctcg tcgcggcgga tctcggccat ccggccgtgc
     7381 acctccggca ggtccatcgc cgggttgagg atgtcgccgc gctcgccacc ggtgagcgtc
     7441 accaccatca cgcggacgcc ctcgtcgacg tagcgggcca tggtggcggc gcccttgctg
     7501 gactcgtcgt ccgggtgggc gtgcaccgcc atcaaccgca gttcgctcaa cctgtccctc
     7561 atcacctggt cactgacatt gccgagacgt ttcaaccatt cgggtagccc tatagttcca
     7621 gttttgtaga actatccgac ggacgggcat gcaaaacgac atgatcgagc gccccgccga
     7681 ccgctacggg cggcagcggc tgtcgcgcgg caaccgccgc tggattgcca ttgggctggg
     7741 cgtgctggtg ctcgtggtcg ggattgtgat cgccgtgatt gcttcccagc ggctcggcag
     7801 cggggacgtc aaaggcgagc tgggcggcta tcagctggtc gacgacgaaa cggtgcaggt
     7861 gacgatcacc gtgacccgcg acgacccgtc ccggccggtg gtgtgcatcg tgcgggcccg
     7921 gtcgatcgac ggcagcgaaa ccggccgccg cgaggtgctg gtggcgccgt cgacgcagaa
     7981 gaccgtgcag gtgaccacgg tcgtcaaggc cagccggcca ccggtggtcg gcgacgtcta
     8041 cggctgcggc accgatgtgc cgtcctacct ggtggcctcc tagaacacac cgcgcaagtc
     8101 agggcaacaa acggcgaaca gcgagaacgt gaaaaatcgc tgtcccgacg gtgctaaaat
     8161 ggtccgatac acggttcccg ctgggaccgt gtattgctgc ttttagtgcg cggattaggc
     8221 gttgacgatc gcggcaatac acgacgagag ctcccggcgc cgtagacacg aaacttctta
     8281 cgcgaacgag cgcgagagca acgacgacag gagcgcgacg acatgaccga cacccaggtc
     8341 acctggttga cccaggaggc atacgaccgg ctgaaggcag agcttgatca gctgatcgcc
     8401 aatcgaccgg tcatcgcagc cgagatcaac gaccgccgcg aggaaggcga cctgcgcgag
     8461 aacggcggct accacgccgc ccgtgaggag cagggccagc aggaggcccg gatacgccag
     8521 ctgcaggagc tgctgaacaa cgccaaggtg ggcgaggcgc ccaagcagtc cggtgtggcg
     8581 ctgcccggct cggtggtcaa ggtgtactac gacgacaaca agagcgacac cgagacgttc
     8641 ttgatcgcca cccgccagga aggcgtgtcc gacggcaagc tcgaggtgta ctcgccgaac
     8701 tcgccgctgg gcggcgcgct gatcgacgcg aaggtcggcg agacgcgcac ttatcaggtg
     8761 cccaacggca acaccgtcaa agtgacgctg gtcagcgcgg agccctacca ctcctaagtc
     8821 gcgactgcgc cgcggactcc taattcgcgg ccccgccgcg gggcggttta cagtccgcct
     8881 atggcgcaga tcgccgagga cttgtttctg ctgctgctgg acaacgcgtc cgctcagccc
     8941 ggcctcgacc gtgtccgccg ccagcgggtg ttgtccgccg cggtgctgct ggatcttgcg
     9001 cacgcatgcc gaattcggcc tgcgatggcc ggtgagcccg tcgagcctgg ccggctcgtg
     9061 gcactcgccg ttcccgggcc gatggacccg gtcgttgcgc cggcgttcca actgctgcag
     9121 cgacggccat tgcgtcctgc caccgccgtc aagcggctgt ccaaacacac cgaagagaac
     9181 ctcgccaact atctggagta catgggccag atccggcgtg ttcggttgaa ctccaagcgg
     9241 ttcagccacc cgtacgcgtg gccactgacc aacagggatc gggtcggtcc cgcgcgctcg
     9301 gcgctgctcg gggcgttgtt cgaccgcagg ccgcccaccc cgacgacggc ggcgatcatc
     9361 tcgttgttgc acgcggtcga cgggctcggt gcactgctca gcctcaacga ccgcggctgg
     9421 cggtgggtgc acgccagggc gggcgaaatc gccatgggca gctgggtgga cgagtatccg
     9481 accgcgatgc ccgaagtgaa cctggcgatc accgcgtcgg cggtgcgagg ggcgctagcc
     9541 tagcgcctgc tcgaggtcgg cgagcaaatc cgcggcatct tcgatgccga cggacagccg
     9601 aaccaggtcg tcgggcacct ccagctgcga gcccgcggtc gatgcgtgcg tcatcgcacc
     9661 cggatactcg atcagcgact ccaccccgcc cagcgattcg gcgagaatga aaacctctgt
     9721 ccgagagcag aagtcgtgcg cggcctgcgg cccgccgcgc atgcgtaccg acaccatgcc
     9781 gccgaacgcg ctcatctgcc gcttcgcgac ttcgtggccg gggtggctgg gcagccccgg
     9841 atacagcacg gtgtcgattg cgggatggcc ggcgaggaac tcggctaccg ccccgccgtt
     9901 ctcgctgtgc ttctgcatcc gcagcaccag ggtcttgagc ccccgcatgg tcaggtacgc
     9961 gtcgaacggg ccgggcaccg cgcccgcgcc gttctgcagg aatgcgaatg cggtgtcgag
    10021 ttcttcgtcg ttggtcagca gcgcgccgcc gaccacatcg gagtgtccgc cgatgtactt
    10081 ggtggtcgaa tgcagcacga tgtcggcgcc caagagcaac ggctgctgta atgcgggaga
    10141 cgcaaaagtg ttgtccacca acacctttat gttcgacggg cctgccagtt gggatatcgc
    10201 ggcgatgtcg gcgatcgaga gcaacgggtt ggttggcgtc tccaaccaga tcaaccgagt
    10261 cttcggggtg atcgcagcgc ggaccgcatc gacatcggac agcgccaccg ccgtgtagtc
    10321 gatcccccac tgcgtgaaca ccttgtcgat cagcctgaag gttccgccgt acgcgtcgtc
    10381 gggcatgacg acgtgatccc cgggccgcag caccgcgcgc agcacgcagt cggtggcggc
    10441 catcccggag gcaaacgcgc gcccgaacgc cgcgtcctcc accgcggcca acgacgcctc
    10501 gagcgccgcg cgcgtcgggt tgccggtgcg ggcgtactcg aacccgccgc gcagcccgcc
    10561 gacgccgtcc tgcgcgaacg tcgagctcgc atagatcggt gcgttgacgg cgccggtgcc
    10621 tgggtcgggc cgaaatcccg cgtggatggc cttggtggcc aagccctgaa acctctgttg
    10681 ttcggtcatc acggatcagg gtaacgatgc gtaatgggcg acccgcccgg tgtagacatg
    10741 gaccatggct acccgtctcg aagacttgct tgacctcgac tcgatgctga cgcccgaaga
    10801 catcgagctg cgccagacgg tgcgccggtt cggcgagcag cggctgcgcc cacacatcgc
    10861 cgactggttt gaggccggcg aggtgccggt ccgcgagctc gccgccgacc tgggcaagct
    10921 gggtgtgctg ggcatgcatc tgaagggcta cggctgcggc ggctcgacag cgaccgccta
    10981 cgggttggtg tgccaggagc tcgaggcggt cgattcgggc ctgcgcagcc tggtgtcggt
    11041 gcagggctcg ctggccatgt tcgcgatcca ccggttgggc agcgaggagc agcgcaaaca
    11101 gtggctgccc gcgatggcca cgggcgacgt catcggctgc ttcgggttga ccgagccgga
    11161 cttcggctcc aaccccggcg gcatgcgcac cacggcccgc cgcgacgggt cggactggat
    11221 cctcaacggt tcgaagatgt ggatcaccaa cggctcggtg gccgacgtcg cgatcgtgtg
    11281 ggcccgctcc aaggaaggca tcgtcggctt cgccgtgcct gcgggcacgc ctggcttttc
    11341 ggctcgagac atgacgcaca agctgtcgct gcgcgcttcg gtcacctccg aactgcatct
    11401 cgacgacgtg cggctgcccg ccgacgccca gctgcccgac gcaaacggtt tgtccggtcc
    11461 gctgagctgc ctatcggagg cgcgg
//
LOCUS       DASRJA010000553         5971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_717010,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000553 DASRJA010000000
VERSION     DASRJA010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717010"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /gene="atpB"
                     /locus_tag="VFC01_24500"
     CDS             <1..737
                     /gene="atpB"
                     /locus_tag="VFC01_24500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZN82809.1"
                     /translation="ESGIEVGHHTTANWFGLTVNTDTVLATAIAAVIVLALAFFLRAK
                     VTSTGVPNGVQLFWEAITVQLRNQIEAAIGMKIAPFVLPLAVTLFVFILIANWLSVLP
                     VQYTDSSGAIHELIKPPASDINFVLALALFVFICYHAAGVWRRGPLGHPLKLLKGHVA
                     LMAPINLVEEIAKPISLSLRLFGNIFAGGILVALIALFPPYILWLPNAIWKSFDLFVG
                     AIQAFIFALLTILYFSQSMELEEDHH"
     gene            802..1047
                     /locus_tag="VFC01_24505"
     CDS             802..1047
                     /locus_tag="VFC01_24505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HZN82810.1"
                     /translation="MDPTIAAGALIGGGLIMAGGAIGAGIGDGIAGNALIAGIARQPE
                     AQGRLFTPFFITVGLVEAAYFINLAFMALFVFATPVA"
     gene            1056..1565
                     /locus_tag="VFC01_24510"
     CDS             1056..1565
                     /locus_tag="VFC01_24510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZN82811.1"
                     /translation="MADPNVVLLAAEEGGGTNNFLIPNGTFFFVLIIFLIVLGVIGKW
                     VVPPISKVLRERDAMVKKTVEDSRKAADQFTAADDDYRAEMAKARGEASKLRDGARAE
                     ARKVLEDMRGRASDEVSSTLQEADSELKDQALSIQADLRSSVDTLSTTLATRVLGVEI
                     NAATSASGR"
     gene            1570..2907
                     /locus_tag="VFC01_24515"
     CDS             1570..2907
                     /locus_tag="VFC01_24515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B/delta"
                     /protein_id="HZN82812.1"
                     /translation="MSTFIGQLVGFAVIVFVIWRYVVPPVRRMMANQQENVRKQLEDS
                     ADAKKRLDEAEKAHEKALEQAKAEAKQVTEEARVDAERIVEQLRVQADAEVERIKVQG
                     AQQVQLLRAQLIRQLRQDLGVESISRAGELVREHVSDPDSQSATVDRLIDELDAMAPS
                     EAVIEDRVTARLRAASRESLAQLVAKFDEVTADLKPAQLSEVGDDLASVTKLLARESI
                     LTRHLADPADDPAPKVGLLETLLEGKVGDTALEVLKAAVSGRWSSDANLVDAVEHTAR
                     LALLVRAEREDAAGDVEEQLFRFARVLDAQPRLTTLLSDYSAPAEGRVKLVRDVLDNG
                     SGVNATTAQLLSQTVELLRGERADEAVLDLAELAVARRGEVVAHVGAAADLNDEQRSR
                     LTEVLTRIYAHPVSVQLHINPELLGGLNIAVGDEVIDGTLSSKLAAAETKLPD"
     gene            2960..4606
                     /gene="atpA"
                     /locus_tag="VFC01_24520"
     CDS             2960..4606
                     /gene="atpA"
                     /locus_tag="VFC01_24520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZN82813.1"
                     /translation="MAELTISADDIQGAIEDYVSTFTADTAREEIGTVVDAGDGIAHV
                     EGLPSVMTQELLEFPGGVLGVALNLDEHNVGAVILGDFEKIEEGQQVKRTGEVLSVPV
                     GDKFLGRVVNPLGQPIDGKGDIEAETRRALEIQAPSVVQRQGVSEPLQTGIKAIDSMT
                     PIGRGQRQLIIGDRKTGKTAVCVDTILNQRQNWESGDEKKQVRCVYVAIGQKGTTIAS
                     VYNTLEEGGAMEYTTIVAAPASDSAGFKWLAPYTGSAIAQHWMYDGKHVLIVFDDLSK
                     QAEAYRAISLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDELGGGSLTGLPIIETKA
                     NDISAYIPTNVISITDGQCFLETDLFNQGVRPAINVGVSVSRVGGAAQIKAMKEVAGS
                     LRLDLSQYRELESFAAFASDLDATSKAQLERGARLVELLKQPQHQPLPAEEQVVSIFL
                     GTEGHLDSVPVEDVGRFEAEFLDHVRASEGEILAQIRESGKLGDDLSDKLVEAINQFK
                     KGFAASDGSSVVPDEHVDALAEEDLEKESVKVRKPAPKKK"
     gene            4613..5536
                     /locus_tag="VFC01_24525"
     CDS             4613..5536
                     /locus_tag="VFC01_24525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HZN82814.1"
                     /translation="MAATLRELRGRIRSAGSIKKITKAQELIATSRIGRAQSRLQAAR
                     PYALEITSMLTTLAAEAALDHPLLVEREDAKRAGVLVVTSDRGLCGAYNSNVFRRAEE
                     LFSLIRQEGKTPIVYAVGRKALNYYRFRNWNVTDSWNGFSEQPTYENAQEIASTLVDA
                     FLAGADDHGDDPGPDGVLGLDELHIVYTEFKSMMSQNAVAHRIAPMVVEYVEEEIGPR
                     TLYSFEPDATTLFDALLPRYLATRIYAALLESAASELASRQRAMKSATDNADDLIKAL
                     TLEANRERQAQITQEISEIVGGANALAGARR"
     gene            5558..>5971
                     /locus_tag="VFC01_24530"
     CDS             5558..>5971
                     /locus_tag="VFC01_24530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215826.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZN82815.1"
                     /translation="MTATAEKTDKATNKDTNGRVVRVTGPVVDVEFPRGSVPELFNAL
                     HAEVTFEELAKTLTLEVAQHLGDSLVRCISLQPTDGLVRGVDVVDTGKSISVPVGDGV
                     KGHVWNALGVCLDEKDYGNDFDRWPIHRKPPAFDQL"
BASE COUNT         1119 a         1895 c         1957 g         1000 t
ORIGIN      
        1 ccgaatccgg catcgaggtc ggccatcaca ccacggcgaa ctggttcggc ctcaccgtca
       61 acaccgacac cgtgctggcc accgcgatcg ccgcggtgat cgtgctggcg ctcgcattct
      121 tcctgcgcgc caaggtgact tcgacgggtg tgccgaacgg tgtgcagctg ttctgggagg
      181 cgatcacggt tcagctgcgc aaccagatcg aggccgcgat cggcatgaag atcgcgccgt
      241 tcgtccttcc gctggctgtc acgctgttcg tgttcatcct catcgccaac tggctttcgg
      301 tgctgccggt gcagtacacc gattccagcg gtgcgataca cgagctgatc aagccgcccg
      361 cgtccgacat caacttcgtg ctggcgctcg cgctgttcgt gttcatctgt taccacgcgg
      421 ccggtgtgtg gcgccgtggt ccgctgggcc acccgctcaa gctgctcaag ggccacgtcg
      481 cgctgatggc gccgatcaac ctcgtcgagg agatcgccaa gccgatctcg ttgtcgctgc
      541 gactctttgg caacatcttc gccggcggca tcctggtcgc gctgatcgcg ctgttcccgc
      601 cgtacatcct gtggctgccc aacgcgatct ggaagagctt cgacttgttc gtcggcgcga
      661 tccaggcgtt catcttcgcg ctgctgacga tcttgtactt cagccaatcg atggagctcg
      721 aagaggacca tcactagccg accgaacaag caggacccac aacctggtag gaccactgcc
      781 agttatcaag gaggataagg aatggatccc acaatcgctg ccggcgcgct catcggcggt
      841 ggactgatca tggccggcgg cgcaatcggc gccggtatcg gtgacggcat cgcgggtaac
      901 gcgctgatcg ccggtatcgc ccggcagccg gaggcgcagg gccgactctt cacgccgttc
      961 ttcatcacgg tcggtctggt tgaggcggca tatttcatca acctggcgtt catggcgctg
     1021 ttcgtgttcg caaccccggt tgcttagtgc ccgccatggc tgacccgaac gtcgtccttc
     1081 tggcggctga ggaaggtggt ggcaccaaca acttcctcat tcccaacggc accttctttt
     1141 tcgtgctgat catcttcttg atcgtgctcg gcgtcatcgg caagtgggtc gtgccaccta
     1201 tttccaaggt gctgcgcgaa cgcgacgcca tggtgaagaa gaccgtcgag gacagccgca
     1261 aggcagccga ccagttcacc gccgccgacg acgactaccg ggccgagatg gccaaggcgc
     1321 gcggggaagc ctcgaaactt cgtgacgggg cgcgagcgga agcgcgaaag gtcttggagg
     1381 acatgcgagg ccgggcatcc gatgaagtgt cgtcgacgct gcaggaggcc gactcggagc
     1441 tgaaggacca agccctgtct atccaggcgg acctgcggtc gtcggtcgac accttgtcca
     1501 cgaccctggc aacccgggtg ctcggcgtcg agatcaacgc ggccacatcg gcttcgggac
     1561 ggtaaggcaa tgtcgacatt catcggtcag ctcgtagggt tcgcggtcat cgtgttcgtg
     1621 atttggcgct acgtcgttcc gccggtgcgc cgcatgatgg ccaaccagca ggaaaacgtg
     1681 cgcaagcagt tggaggacag cgccgacgcc aagaagcggt tggacgaagc cgagaaggcg
     1741 cacgagaagg ccctcgagca ggccaaggct gaggccaagc aggtgaccga agaggcgcgg
     1801 gtcgacgcgg agcgcatcgt cgagcagctg cgtgtacagg ccgacgccga ggttgagcgc
     1861 atcaaagtgc aaggcgccca gcaggttcag cttctgcgag cccagttgat ccgccaactg
     1921 cggcaggacc tcggtgtcga gtccatcagc cgggccggcg aattggtcag agaacacgtc
     1981 tcggatcccg attcgcaatc ggctaccgtc gaccgattaa tcgacgagct cgacgcgatg
     2041 gcaccgtccg aagcggtgat cgaagaccgg gtgacagcgc ggctgcgcgc ggcgagccgg
     2101 gagtcgctgg cacagctggt cgcgaagttc gacgaggtga cggccgacct caaaccggcc
     2161 cagttgtctg aggtcggcga cgatttggcc tcggtcacca aactgctggc gcgcgagtcg
     2221 atcctgacgc gtcatctcgc ggacccggca gacgaccccg cgccgaaggt cggcctgctc
     2281 gagacactgc tcgaaggcaa ggttggcgac acggcgcttg aggtgctgaa agccgcggtg
     2341 tcgggacgct ggtcatccga cgcgaacctg gtcgacgccg tggaacacac cgcgcggctg
     2401 gcgctgctgg tacgcgctga gcgcgaagac gcggccggcg atgtcgaaga gcaactcttc
     2461 cggttcgccc gcgttctcga tgcgcagccg cgcctgacca ccctgctgag cgactacagc
     2521 gcgcctgccg agggccgggt caagctggtg cgtgatgtcc ttgacaacgg gtccggcgtg
     2581 aatgccacca cggcccaact gctttcgcag actgtcgagc tgcttcgggg cgaacgcgcc
     2641 gacgaggccg tgctcgatct ggccgaactc gcggtggccc gccgaggcga ggtggttgcg
     2701 cacgtcggcg cagcggccga cctgaacgac gagcagcgct cacggctgac agaggtattg
     2761 acccgcatct acgcgcaccc ggtttccgtg cagctgcaca tcaaccccga gctgctgggc
     2821 gggctcaaca ttgccgtcgg cgatgaggtg atcgacggca cgctctcatc gaaactggcg
     2881 gccgccgaga ccaagctgcc cgactgaccg acaacaaacg acaagacccc acaccgtaag
     2941 agcaggaaga cgaaaagcca tggcagagtt gaccatctcc gctgacgaca tccagggcgc
     3001 gatcgaggac tacgtctcga ctttcaccgc cgacaccgcg cgcgaggaga tcggtacggt
     3061 cgtcgacgcc ggcgacggta tcgcgcacgt cgagggcctg ccctcggtga tgacgcagga
     3121 gctgctggag tttcctggcg gcgtgctcgg tgtggcgctt aacctcgacg agcacaacgt
     3181 cggtgcggtg atcctcggcg acttcgagaa gatcgaagaa ggccagcagg tcaagcgcac
     3241 cggtgaggtt ctgtcggtgc ccgtcggtga caagttcctc ggccgggtgg tcaatccgct
     3301 gggccagccg atcgacggca agggcgacat cgaggcggaa acccgtcgcg cattggagat
     3361 tcaggcgccc tcggtggtgc agcgccaagg cgtgagcgag ccgctgcaga ccggcatcaa
     3421 ggccatcgac tcaatgaccc cgatcggccg tgggcagcgt cagctgatca tcggtgaccg
     3481 caagaccggc aagaccgcgg tctgtgtgga taccatcctg aaccagcgcc agaactggga
     3541 gtccggcgac gagaagaagc aggtgcgctg cgtctacgtc gcgatcggtc agaagggcac
     3601 caccatcgcc agcgtctata acacgctgga ggagggcgga gcgatggagt acacgaccat
     3661 cgtcgccgcg cccgcttcgg actccgccgg cttcaaatgg cttgcgccgt acaccggttc
     3721 agccatcgca cagcactgga tgtacgacgg caagcacgtg ctgatcgtgt tcgacgacct
     3781 gtccaagcag gccgaggcct accgcgcgat ctcgctgctg ctgcgtcgcc caccgggccg
     3841 cgaggcctac ccgggcgacg tgttctacct gcactcgcgg ctgctggagc gctgcgccaa
     3901 gctatccgac gagctgggcg gcggttcgct gaccggtctg ccgatcatcg agaccaaggc
     3961 caacgacatc tcggcctaca tccccaccaa cgtcatctcg atcaccgacg gccagtgctt
     4021 cctcgagacc gacctgttca atcagggtgt gcgcccggcc atcaacgtcg gtgtctcggt
     4081 gtcccgcgtg ggtggcgccg cacagatcaa ggcgatgaaa gaggtcgctg gctcactgcg
     4141 gctggacctg tcgcagtacc gcgagctgga gtcgttcgcc gcgttcgcct ccgacctgga
     4201 cgccacctcg aaggcgcagc tggaacgcgg tgcccggctg gtcgagctgc tcaagcagcc
     4261 gcagcaccag ccgctgcccg ctgaagagca ggtcgtgtcg atcttcctcg gcaccgaggg
     4321 ccatctggac tcggttccgg tcgaggacgt tggccgcttc gaggctgaat tcctcgatca
     4381 cgtgcgggcg tcggagggag agatcctcgc gcagatccgc gaatcgggga agctcggtga
     4441 cgacctcagc gacaaactgg ttgaggcgat caatcagttc aagaagggct tcgctgcatc
     4501 cgacggcagc tcggtggtcc ccgacgagca tgtcgatgcg ctcgccgagg aagacctcga
     4561 aaaggaatcg gtgaaggttc gcaagcccgc accgaagaag aagtagtcaa cgatggcggc
     4621 aacacttcgg gagttacgcg gacgtatccg ctcggcaggg tcgatcaaga agatcaccaa
     4681 ggcccaggag ctgattgcga cgtcacgcat cggccgggcc cagagccggc ttcaggctgc
     4741 gcgcccgtac gccttagaga tcacctccat gttgaccacg ctggcggcgg aggcggcgtt
     4801 ggatcatccg ctccttgtcg agcgggagga cgccaagcgg gcaggcgtgc tcgtcgtgac
     4861 gtccgaccgt ggtctgtgcg gcgcgtacaa ctcgaacgtg ttccgtcgcg ccgaggagct
     4921 gttctcgctg atccggcagg agggcaagac gccgatcgtc tacgcggtgg gacgaaaggc
     4981 gttgaactac taccggttcc gcaactggaa tgtcaccgac tcgtggaacg ggttctccga
     5041 gcagccgacg tatgagaacg ctcaggagat cgcctcgacg ctggtcgacg cgttccttgc
     5101 cggcgccgac gaccatggtg acgatccggg tcccgacgga gtccttggac tcgacgagct
     5161 gcacatcgtc tacaccgagt tcaagtcgat gatgtcgcag aacgcggtcg cgcaccgcat
     5221 cgcgccgatg gtggtggaat acgtcgagga agagatcgga ccgcgcacgc tgtactcgtt
     5281 cgagcccgac gccacaacgc ttttcgatgc gctgctgccg cggtacctgg cgacccgaat
     5341 ctatgccgcg ctgctcgagt cggcggcgtc ggagctggca tcgcgccagc gcgcgatgaa
     5401 gtcggccacc gacaacgccg acgacctcat caaggccctg accctcgagg ccaaccgcga
     5461 gcggcaggcc cagatcaccc aggaaatcag cgagattgtc ggtggcgcga acgcgctggc
     5521 cggcgccaga cgttagcccg ttagaaagaa gaaaagaatg actgctaccg ccgaaaagac
     5581 cgacaaggca acgaacaaag acacaaacgg tcgcgtggtg cgggtgaccg gccccgtggt
     5641 cgacgttgag ttcccgcgcg gctcggtgcc cgaattgttc aatgcactgc acgccgaggt
     5701 cacgttcgag gagctcgcaa agacgctgac gttggaggtg gcccagcatc tcggcgacag
     5761 cctggtgcgt tgcatctccc tgcagccgac cgacggcctg gtccgcggcg tcgacgttgt
     5821 cgacaccggc aagtcaatct cggtgccggt cggcgacggg gtgaagggcc acgtctggaa
     5881 cgcccttggc gtctgcctgg acgagaagga ttacgggaac gacttcgacc gctggccgat
     5941 tcaccgcaag ccgccggcgt tcgaccagct c
//
LOCUS       DASRJA010000554         8424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3712985,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000554 DASRJA010000000
VERSION     DASRJA010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3712985"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFC01_24535"
     CDS             <1..585
                     /locus_tag="VFC01_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HZN82816.1"
                     /translation="IADHLIGHFVDQARKSGASWTEIGKCMGVTKQAAQKRFVSKADA
                     ALDPEAGFGRFTPRARNVVVVAQNRANKAGNNEIGPHHLLLGLFSDPDGLAPKLLAGQ
                     GVDADKVTRAVTLPPRVDDVPTLIPFNGAAKKVLELTFRQALRLGHNYVGTEHIFLAL
                     YEAEDDDGTLHQLGVDRDRFERELVAALSEYTKA"
     gene            627..1103
                     /locus_tag="VFC01_24540"
     CDS             627..1103
                     /locus_tag="VFC01_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN82817.1"
                     /translation="MRPFEDVVAEHGATVLRVCRAVVGPVDAEDAWSETFLSALRAYP
                     DLPADANVEAWLVTIAHRRAIDVGRAKSRRPVPTETLPARPAAHADPAARDPDLWTAL
                     QRLPTKQRQAVAYHHIAGLPFAEIAELLGNSPDAARRAAADGLKTLRKHYREDETA"
     gene            1100..1693
                     /locus_tag="VFC01_24545"
     CDS             1100..1693
                     /locus_tag="VFC01_24545"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZN82818.1"
                     /translation="MTTNLFDELGADDGALARLHQGLEHAADTGGLLDVAYRTIDTPV
                     GTLLLAATTAGLVRVAYDVEGHDAVLAGLADRISPRVLRAPARLDTAARQIDEYFAKR
                     RTAFEVPVDLRLAEGFRRSVIEHLRAIGYGHRESYAMVAAAIGHPRAVRAVGTACAHN
                     PLPVVIPCHRVVRSDGSTGQYVGGPLAKSTLLDLEAA"
     gene            1735..2157
                     /locus_tag="VFC01_24550"
     CDS             1735..2157
                     /locus_tag="VFC01_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZN82819.1"
                     /translation="MKLNDAARELIGEGTDATLVTINPDGSPQATVVWMALESTPDGD
                     ELVAAHLAEYQKTRNIRRDPRVAVTILSTKHPNQQTPYLTITGTARIEEGGAPALLKK
                     LAKTMLGSDEHFPPPNAPEGLLTRVRIEKVGGLGPGAS"
     gene            2204..2731
                     /locus_tag="VFC01_24555"
     CDS             2204..2731
                     /locus_tag="VFC01_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82820.1"
                     /translation="MRRCFVLLVALLAAVASLVAPPASAEGAPIGNLHDTLRVEYKGI
                     VADVTVHDVLPVDPPPPGYAATGSPRWINQGGPWKAPITIHVIETPTPFAMAKAFSFN
                     GVTPYADAYQPKNTDAPDELQYALRNAPPGATVNGAVYWQVYRALVTNVVLLDRQTGE
                     HLAQWNIWQPGAPLP"
     gene            2736..3269
                     /locus_tag="VFC01_24560"
     CDS             2736..3269
                     /locus_tag="VFC01_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN82821.1"
                     /translation="MAIHVAAAVETDLPELAEVAAVTFPLACPPAVTAENIAAFVDEN
                     LSERRFRDYLADPDRAVLAAREDGRIVGYVMLIRGVPDDDDVQQAVKLRPAVELSKMY
                     VLPDSHGVGISAALMTAALQCATDLDAKCVWLGVNQQNQRAQRFYTKHGFTINGTKTF
                     RLGAGIENDYVMVRPLS"
     gene            complement(3317..4402)
                     /gene="zapE"
                     /locus_tag="VFC01_24565"
     CDS             complement(3317..4402)
                     /gene="zapE"
                     /locus_tag="VFC01_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HZN82822.1"
                     /translation="MRRIAARRYGACVQGSSGVAHLVDRHPSVTPERLIAQLVPPPTF
                     SDVSFETYKPDPAEPSQAAAVQSCRQFCEQAVARRAGKKKLFGRRETLPGVGIYLDGG
                     FGVGKTHLLASSYFDLPEPKAFATFGELTQLAGVFGFVECIDLLADYSAVCIDEFELD
                     DPGNTTLISRLLSELVARGVSVAATSNTLPEQLGEGRFAVQDFLREIQALASIFTSVR
                     IDGPDYRHRDLPPAPDPLSDDEVVACAAEVPGSTVDDFDALCAHLATMHPSRYLTLIE
                     GVTAVFITGVHPIDDQNVALRLVSLTDRLYDAGIPVVASGTKLDTIFSAEMLAGGFRK
                     KYLRATSRLLALTAAGQSGRTSSGEQT"
     gene            4401..5174
                     /locus_tag="VFC01_24570"
     CDS             4401..5174
                     /locus_tag="VFC01_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine reductase family protein"
                     /protein_id="HZN82823.1"
                     /translation="MPDDGAGMQLTVLGPTDSVTEGRLADLYAYPGDLQACWVRGNMI
                     GSLDGGATDDGKAGGLAGAGDRALFALLRHLADVIVVGASTVRVENYSGAQLPVAARQ
                     ERQHRGQAEVPPIAVVTRSGNLDPSARLFTRTEVPPLILTTSRFHDDTRRRLGSVAEV
                     IDASGPEPDSVDAATVLKILADRGLYRVLTEGGPLFLGTLIEEGLLDELCLTVAPILV
                     GGGSKRIVTGLGNVHAKMRRSHVLTDDDGYLYTRYVKGV"
     gene            5200..6765
                     /locus_tag="VFC01_24575"
     CDS             5200..6765
                     /locus_tag="VFC01_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN82824.1"
                     /translation="MRRRRLVRVLGTSTVVLSTLLAGCAPGLAANPRYATDSGAGPQG
                     AETTKPPSGPPPIEAPKNELSWRDCTSRVFGDAAVQPLPGVKLECASYDADLDPINGA
                     TGAVSIGVVRARSAETPQDAGPIVMTTGSDLPSSEHLPVWLSRAGADVLKAHPIVAVD
                     RRGIGMSGALDCRDLFDRQEMLDQAQFQSGDDPVANLGAITQTATTSCTDTIAPGDSA
                     YDNSHAAEDIERLRSTWDVPTIALLGIGNGAQVALAYAGSHPNKVARLVVDSPLPLGI
                     AAEAAMEQRVKGEQTALDAWAAQCVATNCPLAPDPKGAIDALLAAARNGDGPGGASVA
                     TVVDAITTALAYPRGDRVNSGNDLAAAVAAARSGNTNQMTNLITAAETLRQTDGQFVN
                     SCSDSLNRPTPDRVRELVVAWPKLYPQFGAVGALNLVKCLNWPSGSAPQDPKNLKTPV
                     MLLGGQNDPIVGNEGVAAVAATVINAGTTSKRVIWQGIGHGASIYSACALPPVIGYLD
                     SGNLPPTDTFCPA"
     gene            6787..8076
                     /gene="aftC"
                     /locus_tag="VFC01_24580"
     CDS             6787..8076
                     /gene="aftC"
                     /locus_tag="VFC01_24580"
                     /EC_number="2.4.2.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranan 3-O-arabinosyltransferase"
                     /protein_id="HZN82825.1"
                     /translation="MYGALVAAPDSIRTGLLKAFAPRTSPPSTATVLRSILWPIAILL
                     IIHRSYVLATNGYITDDYGPVYRAVVNFKMGWDIYNEHFDQVDPHYLYPPGGTLIMAP
                     FGYLPVDASRYWFITFNTIAIVIAAFLLVKLFKFSWSSVALPALLVAMFCTESVVNTL
                     VFGNINGCILLLEVLFFRWLLDGDKKHEWWAGVAIGLSLVVKPLLGPLLLLPLLNRQW
                     RALVAAVIVPLVFNAAAWPLVSDPMNFVTGTLPYIFKTRDYFNSSIQGNGVYYGLPLW
                     LILLLRIVFLALVIASLWLLNRYYRTRDPLFWMTTSSGVLLIGSFLVLSLGQGYYSMM
                     LFPFLMTVVLPNSVIRNWPAWLAIYGFMSADRWLLGHWPTTGRFLEYMRFTYGWSLML
                     IVVFCVLYFRYLDAKADDRLDDGIDPPWMKQRASVDA"
     gene            8073..>8424
                     /gene="msrB"
                     /locus_tag="VFC01_24585"
     CDS             8073..>8424
                     /gene="msrB"
                     /locus_tag="VFC01_24585"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZN82826.1"
                     /translation="MTTPAPKVQLTDDEWRKKLNPEEFAVLRQAGTERPFTGEYTDTK
                     TEGVYACRACGAELFRSSEKFESHCGWPSFFDPANSDAVILRTDDSLGMHRVEVICAN
                     CHSHLGHVFEGEGYP"
BASE COUNT         1427 a         2926 c         2683 g         1388 t
ORIGIN      
        1 atcgccgacc acctgatcgg ccacttcgtg gatcaggctc gcaagtccgg ggcgtcgtgg
       61 accgagatcg gcaagtgcat gggcgtgacc aagcaggccg cgcagaaacg tttcgtgtcc
      121 aaggccgacg ccgccctcga cccggaggcg ggcttcggcc gattcacccc tcgggcccgc
      181 aacgtcgtcg tcgtcgctca gaacagggcc aacaaggccg gcaacaacga gatcgggccc
      241 catcacctgc tgctcggcct gttttcggac ccggacggcc tcgcccccaa gctgctcgcc
      301 ggtcagggcg tcgatgccga caaggtgacc agggccgtca ccctgccacc ccgggtcgac
      361 gacgtgccca cgctgatccc cttcaacgga gcggcaaaga aggtgctgga actgaccttc
      421 cgccaagcac ttcggttggg ccacaactac gtcggcacgg aacacatctt tttggcgctg
      481 tatgaggccg aggacgacga cggcacgcta caccagctgg gtgtcgacag agatcgcttc
      541 gagcgcgagc tcgtcgcggc actctcggaa tacacgaaag cctgatctca catcctttag
      601 cgccgcgccg tcttcatgat gtggaggtgc gaccgttcga ggatgtggtg gccgagcacg
      661 gcgcgaccgt gctgcgggtg tgtcgcgccg tcgtcggccc cgtcgacgcc gaggacgcct
      721 ggtccgagac gttcctgtcg gcactgcgcg cctatcccga cctgcccgcc gacgccaacg
      781 tcgaggcctg gctcgtcacg atcgcccacc gccgagcgat tgacgtcggg cgcgcgaaat
      841 cccgccgccc cgtgcccacc gaaaccctgc ccgcgcgccc cgccgctcat gccgatcccg
      901 ccgcgcgcga ccccgacctc tggaccgcgc tgcagcggct gccgaccaaa caacgccagg
      961 cggtggccta ccaccacatt gcgggcctgc cgttcgccga gatcgccgaa ctgctcggca
     1021 actcccccga cgccgcgcgg cgcgccgccg ccgatggact caagacactg cgcaagcact
     1081 accgtgagga cgaaacagca tgactaccaa tctttttgac gaactgggcg ccgacgacgg
     1141 cgcactggcc cggctgcacc aagggctgga gcacgccgcc gacactggcg ggctgctcga
     1201 cgtcgcctac cgcacgatcg acacacctgt cggcacgcta ctgttggccg ccaccaccgc
     1261 aggactcgtg cgggtcgcct acgacgtgga gggccacgac gccgtgctgg ccgggctggc
     1321 cgacaggatc agcccgcgag tgctgcgcgc gccggcacga ctggacaccg cggcccggca
     1381 gatcgacgag tatttcgcca agcgccgcac cgcctttgag gtgccggtgg atctgcgact
     1441 cgcggagggt ttccgacgca gcgtcatcga acatctgcgc gccatcggct acggccaccg
     1501 cgagagctac gcgatggtgg ccgccgcgat cggtcatccg cgtgcggtgc gggccgtcgg
     1561 cacggcctgc gcccacaatc ccctgccggt cgtgatcccc tgccaccgcg tcgtgcggtc
     1621 agacgggtcg acgggccagt acgtcggcgg gccattggcc aagtcgacgc tgctcgacct
     1681 tgaggccgcc tgaaaacggg aatgaatccg cgggcctggc ggtagaacag gaacatgaaa
     1741 ctcaacgacg ccgcccgcga gctcatcggc gagggcaccg acgcgacact ggtcacgatc
     1801 aacccggacg ggagcccgca ggcaaccgtc gtgtggatgg ccttggagtc caccccggac
     1861 ggcgatgagc ttgtcgccgc acacctggcc gaataccaaa agacccgtaa cattcgccgc
     1921 gatccgcggg tggccgtcac gattctgtcc accaagcacc ccaaccagca gacgccttat
     1981 ctgacgatca ccggaacggc ccggatcgag gagggcggcg cgcccgcgct tctcaagaag
     2041 ctggccaaga cgatgctggg gtccgacgag cacttcccgc cgccgaacgc gccggaaggc
     2101 ttgctcaccc gcgtccggat cgagaaggtc ggcgggctcg gtccgggggc atcctgacgc
     2161 ccaaccggct gtaacacgtt tcagtttgtg ccatcatgcg ttgatgcgcc gctgcttcgt
     2221 gctcctcgtc gctctgctgg cggccgtcgc cagcctcgtg gccccacccg cgtcagcgga
     2281 gggtgcgccg atcggcaatc tccatgacac cctgcgcgtc gagtacaagg gcatcgtcgc
     2341 cgacgtgacc gtccacgatg tgctgcctgt tgatcctccg cctccgggct atgcggccac
     2401 cggttcgccg cgctggatca accagggcgg tccgtggaag gcgccgatca cgatccatgt
     2461 catcgagacg cctaccccgt tcgcgatggc caaggcgttc tcgttcaacg gcgtcacgcc
     2521 ctacgccgac gcgtatcagc ccaagaacac cgatgcgccc gatgagctgc aatacgcgct
     2581 gcggaacgct ccgcccggcg ccacggtcaa cggcgccgtg tactggcagg tctatcgcgc
     2641 cctggtcacc aacgtggtgc tgctcgaccg gcagaccggt gagcacctgg cgcagtggaa
     2701 catctggcag ccgggggcac cgctaccgta gagccgtggc cattcacgtc gccgcggccg
     2761 tcgaaaccga cctgcccgaa ctcgcggaag tcgctgccgt cacgttcccc ctggcctgtc
     2821 ccccggcggt gacggcggag aacatcgccg cattcgtcga cgagaacctg tcggagaggc
     2881 ggttccgcga ctacctcgcc gatcccgacc gtgcggtgct ggccgcacgc gaggacggcc
     2941 gaatcgtggg ttacgtcatg ctgattcgcg gcgttcccga cgacgacgat gtgcagcagg
     3001 cggtcaaact gcgtcccgcc gtcgaactgt ccaagatgta cgtgctgccc gacagccatg
     3061 gcgtgggcat ctccgcggcg ctgatgaccg ccgcactgca atgcgccacc gatctcgacg
     3121 ccaagtgcgt gtggctgggt gtgaatcagc agaaccaacg cgcccaacgc ttttacacca
     3181 aacatggctt taccatcaac ggcaccaaga cttttcggct cggcgccggc atcgagaacg
     3241 attacgtgat ggtgcgcccg ctctcgtgag cgcgagcaga cgcaaaatga cccgacacgc
     3301 cgacgatttg gggcctttac gtctgctcgc cagaagaggt gcgtccgctc tgacccgctg
     3361 ccgtcaacgc cagcagccga gacgtcgccc gcagatactt cttccggaag ccgcccgcca
     3421 gcatctcggc ggaaaagatg gtgtccagct tcgtccccga agccaccacg ggaatgcccg
     3481 cgtcgtagag gcggtcggtc aacgacacca gccgcagcgc gacattctgg tcgtcgatcg
     3541 gatgcacgcc ggtgatgaac accgctgtga cgccctcgat caacgtcaga tagcgtgacg
     3601 gatgcatggt cgccaggtgt gcgcacaacg cgtcgaagtc atccaccgtc gagccgggaa
     3661 cttcggcggc acacgccacc acctcgtcgt cgctcaaggg gtccggcgcc ggcggcaggt
     3721 cgcgatgccg atagtccggt ccgtcgatgc gcaccgacgt gaaaatgctt gccaaagcct
     3781 ggatttcacg cagaaagtcc tgcacggcga agcggccctc accgagctgc tcgggcagcg
     3841 tatttgacgt cgctgccacc gacaccccgc gtgcgaccag ttcagacagc agtcgcgaga
     3901 tcagcgtggt gttgcctggg tcgtcgagct cgaactcgtc gatgcacacc gcgctgtaat
     3961 cggccagcag gtcaatgcat tcgacgaagc cgaacacccc ggccagctgg gtcagttcgc
     4021 cgaaggtggc gaacgccttg ggctccggca ggtcgaagta tgacgacgcg agcaggtgcg
     4081 tcttcccgac gccgaacccg ccgtcgaggt agatgccgac acccggcagc gtctcgcgtc
     4141 tgccgaacag tttcttcttg cccgcccgcc gcgcgaccgc ctgctcgcag aactgtcggc
     4201 acgactgcac cgccgccgcc tgcgacggct cggccggatc cggcttgtat gtctcgaagc
     4261 tgacgtcgga aaaggtcggc ggtggaacca gctgggcgat cagccgctcg ggcgtgacac
     4321 tcgggtgtcg atcaaccaga tgcgcgacac cgctggaccc ctgcacacac gcaccatagc
     4381 gacgtgctgc aattctgcgt atgcctgacg acggtgcggg gatgcagctg acagtgctag
     4441 ggcccaccga ttcggtgacc gagggccgac tcgctgacct gtatgcctac cccggggacc
     4501 tgcaggcgtg ctgggtgcgc ggcaacatga tcggcagtct cgacggcggg gccaccgatg
     4561 acggcaaggc cggagggctc gcgggtgcgg gtgaccgtgc actgttcgcg ctgctgcgcc
     4621 acctcgccga tgtcatcgtc gtcggggcct ccaccgtgcg tgtcgagaac tactccggcg
     4681 cccagcttcc cgtcgccgcg cgccaggagc gtcagcatcg cgggcaagcc gaggtgccgc
     4741 cgatcgccgt ggtgacccgt tcaggcaacc tcgacccgag tgcccggctg ttcacccgca
     4801 ccgaggtgcc gcccctgatc ctgaccacca gccggttcca cgacgacacg cgccgcaggc
     4861 ttgggtcggt cgccgaggtc atcgacgcgt cggggccgga accggactcc gtcgacgccg
     4921 ccaccgtcct gaagatcctg gcggaccgcg ggctgtaccg cgtgctcacc gagggcgggc
     4981 cgttgttcct cggcacgctg atcgaggagg gcctcctcga tgagctctgc ctgacggttg
     5041 cgccgatcct ggtcggcggc gggtcaaagc gaatcgtgac cggtctcggc aatgtgcacg
     5101 ccaagatgcg gcgcagccat gtgctgaccg acgacgacgg ctacctctac acgcgctacg
     5161 tcaagggcgt ctagcgggac catcagtaca ttggtcagca tgcgtcggcg tcgcctggtc
     5221 cgggtccttg gcacctcgac cgtcgtgctg tccactttgc tggccggctg cgcgccaggc
     5281 ctggccgcca atccgcgcta cgcgaccgac tccggcgcgg gcccgcaggg tgcggagacc
     5341 accaaaccac ccagcggccc gccgccgatc gaggcgccga agaacgagct gtcctggcgg
     5401 gattgcacgt cacgggtttt cggtgacgcc gcggtgcagc cgttgcccgg cgtcaaactg
     5461 gaatgcgcca gctacgacgc cgacctcgat ccgatcaacg gcgccaccgg agccgtcagc
     5521 atcggcgtgg ttcgcgcccg ctcggcggag acaccgcagg acgccggtcc gatcgtgatg
     5581 accactggat cggatctacc gtcgtcggag cacctgccgg tatggctgtc ccgcgcaggc
     5641 gcagatgtgc tcaaggcgca tccgattgtt gcggtcgacc gtcgcggcat cggcatgtcg
     5701 ggcgcgctgg actgccgcga cctgttcgac cgtcaggaga tgctcgacca ggcgcagttc
     5761 cagtccggcg acgatccggt cgccaacctc ggtgccatca cccagaccgc caccaccagc
     5821 tgcacggaca ccatcgcgcc cggcgactcg gcctacgaca actcccacgc cgccgaggac
     5881 atcgaacggc tgcgcagcac ctgggatgtg ccgaccatcg cgctgctcgg catcggtaac
     5941 ggcgcccagg ttgccctcgc ctacgccgga tcgcatccca acaaggtcgc gcggctggtg
     6001 gtcgactctc cgctgccgct tggcatcgcc gccgaggccg cgatggagca acgcgtcaag
     6061 ggcgaacaga ccgcgctgga cgcctgggcc gcgcagtgcg tcgccaccaa ctgcccgctg
     6121 gcgcccgacc ctaagggcgc gatcgacgcc ctgctggcgg cagcccggaa cggcgacggg
     6181 ccaggaggtg cgtcggtcgc gacggtcgta gacgccatca ccaccgcgct ggcctaccca
     6241 cgcggcgacc gcgtcaactc aggcaacgac ctcgctgcgg cggtggctgc cgcccgctcc
     6301 ggcaacacca accagatgac gaacttgatc acggccgccg agacgttgcg tcagaccgac
     6361 ggccagttcg tcaacagctg cagcgactcg ctgaaccggc cgacgccgga ccgggttcgc
     6421 gaactcgtgg tcgcctggcc caagctctac ccgcagttcg gcgccgtcgg cgcactcaat
     6481 ttggtcaaat gcctgaactg gccgagcgga tcggcgccgc aggaccccaa gaacctcaag
     6541 actccggtga tgctgctcgg cgggcaaaac gacccgattg tcggcaacga gggcgtagcg
     6601 gccgtggccg cgaccgtgat caacgcaggc accacgagca agcgggtgat atggcagggc
     6661 attgggcacg gcgccagcat ttactcggca tgcgcgctgc cgccggtgat cggctacctg
     6721 gacagcggca acctgccgcc caccgacacg ttctgcccgg cctgacaccg acctttgggg
     6781 ccgtcggtgt acggtgcgct ggtggcggct cctgactcca tccgaaccgg cctgctgaaa
     6841 gcattcgcgc cgcgcacctc cccgccaagc acggcaacgg tgctgcggtc catcctctgg
     6901 ccgatcgcga tcctgctcat cattcatcgc agctatgtgc tggccaccaa cggctacatc
     6961 accgacgact acggcccggt gtaccgcgca gtggtcaatt tcaagatggg ctgggatatc
     7021 tacaacgagc acttcgatca ggttgacccg cactatctct acccgcccgg cggcacgctg
     7081 atcatggcgc cgttcggcta tctgccggtc gacgcttcgc ggtactggtt catcacgttc
     7141 aacacgatcg ccatcgtcat cgcggcgttc ctgctggtga agctgttcaa gttctcgtgg
     7201 tcgtcggtcg ccttgcccgc cttgctcgtg gcgatgttct gtaccgaaag cgtggtcaac
     7261 acactggttt tcggcaacat caacggctgc atcctgctgc tggaggtgct gttcttccgc
     7321 tggctgctcg acggtgacaa gaaacacgag tggtgggccg gggtggccat tgggctatcg
     7381 ctcgtcgtga aaccgcttct ggggccgctg cttttgctgc ccctgctgaa ccgtcagtgg
     7441 cgggccttgg tcgcggcggt aatcgtgccg ttggtgttca acgcggcggc gtggccgttg
     7501 gtcagcgacc cgatgaactt cgtcacaggc acgctgccat atatcttcaa gacgcgggat
     7561 tatttcaaca gctcaatcca gggcaatgga gtctattatg gcctgccgct gtggctgatt
     7621 ctgttgctgc gcatcgtgtt tctggcgctt gtgatcgcga gcctgtggct actgaaccgc
     7681 tactaccgga cccgcgatcc gctgttctgg atgacgacgt cgtcgggtgt gctgctgatc
     7741 gggtcattcc tggtgctgtc gctgggccag ggctattact cgatgatgct gttcccgttc
     7801 ttgatgacgg tggtgctgcc gaattcggtg atccgcaact ggccggcgtg gctggccatc
     7861 tacgggttca tgagcgccga ccggtggctg ctcggacact ggccgacgac ggggcgcttc
     7921 ctggagtaca tgaggttcac ctacggctgg tccctgatgc tcatcgtcgt gttctgcgtg
     7981 ctctacttcc gctacctcga cgccaaagcc gacgaccgac tggacgacgg catcgatccg
     8041 ccgtggatga agcagcgcgc tagcgtggac gcatgacgac tccagctccc aaggtgcaac
     8101 tcaccgacga cgagtggcgc aagaagctca accccgagga gttcgcggtg ttgcggcagg
     8161 ccggcaccga gcggccgttc acgggcgagt acaccgacac caagaccgag ggcgtgtatg
     8221 cgtgccgcgc ctgcggtgcg gaattgttcc gcagcagcga gaagttcgaa tcccattgcg
     8281 gctggccgtc tttcttcgac ccggccaact ccgacgcggt gatcctgcgc accgacgaca
     8341 gcctgggtat gcaccgcgtc gaggtcatct gtgccaactg ccacagccac ctcggccacg
     8401 tctttgaggg cgagggttac ccga
//
LOCUS       DASRJA010000555         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3671689,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000555 DASRJA010000000
VERSION     DASRJA010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3671689"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2190)
                     /locus_tag="VFC01_24590"
     CDS             complement(<1..>2190)
                     /locus_tag="VFC01_24590"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HZN82827.1"
                     /translation="IAECLALNDDATAGLLDGIAGTVFTLGDNAYENGTLGDYQNCYD
                     PTWGRHKARTHPVPGNHDYDPVSSPTAAGYFAYFGAAAGEAGKGFYSYDLGDWHIVAL
                     NNYIPLGAGSEQLTWLQSDLAATTKPCILGYWHEPLYSSGTGIGSGGDSSAWVRPLFQ
                     ALYDAGADVVLTGHRHFYERLSPMNPLGQLDVTNGIREFVVGTGGGAMGNPTNAHPLS
                     ELRFGSANFGVLKMYLYSDSYAWKFLPVPGRTLSDTGLVACHGVSSGVSPSHSTVVAA
                     PSLVPLGSLSTINVTVRNASGDPISGATVQLSVSPAAGSTLTQPAAATGADGVAMGTL
                     RADDATTKVVTATVTLGGQTTALSQSATVTVSVSASVVHTLLTAGTNPANQNVYTTAS
                     ISPAANALITVAVTSRNALGVSPSPVLTGGGMTTWTEVGSAIFDTRTTPLRRVTIYRA
                     MSASPGSGPLTITFAAGQSNAQWIVSQWTGVDASGTNGSGAILQVGSTNGDAVTGLNV
                     ALNPFEHVNNVAYGAFAVTRNVAVIDPGAGFAEISEQTSGETTPGDLQTEWRVNDNTV
                     DATWGSLSSNGAAVALEIKGVGTGDIGVNAGQSSVTVSPATIVAGTATSTITVTARNA
                     IGAAVRGAAVQISATGSGNTISAPAPTDPNGVTTATLSSTVAETKVITVTAGGTTLEQ
                     EPSVQVTNDGPSPTHSTVTAAPTTIPTDSGISTITVVVKDQFDNPIN"
BASE COUNT          370 a          719 c          694 g          407 t
ORIGIN      
        1 cgttgatggg gttgtcgaat tgatctttca ccaccaccgt aatcgtcgag atcccactgt
       61 cggtcgggat cgtcgtcggt gccgcggtca cggtcgagtg ggtcggcgac ggaccatcgt
      121 tcgtcacttg cacggacggc tcctgctcga gcgtggtccc ccccgccgtc accgtgatca
      181 ccttggtttc ggcgaccgtc gagctcaggg tcgcggtcgt cacgccattc ggatcggtcg
      241 gcgccggcgc ggagatcgta ttgccgctgc ccgtcgccga gatctgaacc gccgctcccc
      301 tgacggccgc gccaatcgcg ttacgcgcgg ttaccgtgat cgttgacgtc gcggtgccgg
      361 caacgatcgt cgccggggag acggtgaccg acgattggcc ggcattcacg ccaatgtccc
      421 ctgtccccac ccccttgatt tccagggcca cggccgcgcc gttcgagctc agagaacccc
      481 aggtggcatc gaccgtgttg tcgttcaccc gccactcggt ttggaggtcg ccgggggtgg
      541 tctcacccga tgtttgctcg gagatttcgg caaagcccgc gccgggatcg attaccgcca
      601 cgttccgtgt aacggcgaac gcgccgtacg ccacattgtt cacgtgctca aacgggttca
      661 gcgccacgtt cagtccggtg accgcatccc cgttcgtgct ccccacctgc aggatcgctc
      721 ctgacccgtt cgtaccactc gcgtccaccc cggtccactg cgacacgatc cactgcgcgt
      781 tgctctgtcc cgccgcaaac gtgatggtga gcggcccact ccccggcgac gcgctcatgg
      841 cccgatagat cgtgacccgc cgcagcggcg tcgtccgcgt gtcgaagatc gccgagccca
      901 cctccgtcca cgtcgtcatt ccgccgccgg ttaggaccgg gctgggcgag acaccgagtg
      961 cgttgcgtga cgttacggca acggtaatca gcgcatttgc cgcaggagaa atcgaggcgg
     1021 tcgtgtagac gttctgattt gccggattgg tccctgccgt cagcagcgta tggacgacgg
     1081 acgcagacac ggatacggtc acggtggcag actggctcag ggccgtcgtc tgtccaccga
     1141 gtgtcaccgt cgccgtaacg accttagtcg tggcgtcgtc ggccctcagc gtgcccatcg
     1201 cgacaccgtc agccccggta gcagcggccg gctgcgtgag cgtgctgccc gcggcaggcg
     1261 ataccgacag ctgcaccgtt gccccggaga ttggatcgcc actggcattg cgtacggtga
     1321 cgttgatcgt cgacaggctg cccaggggaa ccaggctcgg cgcggccaca acggttgaat
     1381 gcgatggcga aacgccggaa ctgaccccgt gacacgcaac gaggccggta tcactcagcg
     1441 ttcttccggg gaccgggaga aacttccagg cgtagctgtc ggaatacaga tacatcttca
     1501 ggacaccgaa attcgcgctg ccgaagcgca actcgctcag gggatgggcg ttggtgggat
     1561 tccccatggc ccctccaccc gtgcccacga cgaattcccg aatgccattc gtgacatcga
     1621 gctggcctag cggattcatc gggctgagac gctcgtagaa gtgccggtgc ccggtgagga
     1681 caacgtccgc gcccgcgtca tagagcgcct ggaagagcgg tcgtacccag gcgcttgaat
     1741 ccccgcccga accaatccct gtaccggacg agtagagcgg ctcgtgccag tagccgagaa
     1801 tgcagggctt cgtggtggcg gccaggtcgc tttgcagcca agtcagctgc tcggaaccgg
     1861 cgcccagcgg gatgtagttg ttgagcgcga cgatgtgcca atcgccgaga tcgtagctgt
     1921 aaaacccctt cccggcctcg ccggcagctg cgccgaagta cgcgaaatag cctgccgcgg
     1981 tggggctgga aacggggtcg tagtcgtgat tcccggggac gggatgggtc cgtgccttat
     2041 gacggcccca cgtcgggtcg taacaattct ggtaatcgcc tagcgtaccg ttttcatagg
     2101 cattgtcgcc gagtgtgaac accgtgccgg cgatcccatc gagcagaccg gccgtcgcat
     2161 catcgttgag agcgagacac tcagcgatgt
//
LOCUS       DASRJA010000556         8872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2910266,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000556 DASRJA010000000
VERSION     DASRJA010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2910266"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFC01_24595"
     CDS             <1..215
                     /locus_tag="VFC01_24595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82828.1"
                     /translation="LVHSDEFARMTAVIARGRRLAGSRANAITARVWHLMNLPASTDV
                     QRLRMQVGVLDREIRRLSVQLDRDSR"
     gene            212..1270
                     /locus_tag="VFC01_24600"
     CDS             212..1270
                     /locus_tag="VFC01_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018162059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN82829.1"
                     /translation="MSVVERVRREVERNALRARNGIRLAAGLRPPDVGLTPKEIVWRK
                     GRTELWRYRNEQIRVSPPLLIVYSLFNRSYILDLQPGNSFVEHLLNAGFDVYLLDFGI
                     PDERDAGNTLDDYADEYLPAAVDRVCRVSHDEQVNLLGYCFGGVLSLLYVAHHLDAPL
                     RSLTALTTPADFQRLGQLGDLLGGGLDIETLIDGDGNVPADIVLQGFRTLRPIGEITQ
                     YVDLWERLWSDEYVAAYQSMTGWATEQVPLPGGVARQIVDMVRENPLMTERLVVGGDR
                     VQLSDITVPFLHVLASRDHIIPEAAAAPLTGLVGSRDKHELRLDAGHVGLLVGKTAAK
                     TTLPTIIDFLKQRSRVKP"
     gene            1267..1827
                     /locus_tag="VFC01_24605"
     CDS             1267..1827
                     /locus_tag="VFC01_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009677283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN82830.1"
                     /translation="MNIVACAAQHGRRLTELFGELSERDLTLIKENLTDPVAVRRWTA
                     RPGQQWVALDGTHIVGYAAVRPLPEWSDHVGELRLVVDPARRRVGVGSALARHALIEA
                     VRAGRRKLVVELAADQDNALAMFASLGFTGEALLRDHIRDRNGEFHDLVMLAHFVDDT
                     WAGMGSIGLSDELGLPQAASANGAVR"
     gene            1824..2588
                     /locus_tag="VFC01_24610"
     CDS             1824..2588
                     /locus_tag="VFC01_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN82831.1"
                     /translation="MTILDRFRIDDRVAIVTGASSGLGVAFAAALAEAGADVSLGARR
                     EDRLAATRKLVEDAGRRAITVRTDVASPDDCRVLVDATVHAFGRVDILVNNAGIGTSV
                     PATRETPEQFRSVIDVNLNGCYWMAQACARVMQPGSSIINIASVLGLTSAGIPQAAYS
                     ASKAGLIGLTRDLAQQWTGRKGIRVNALAPGYFESEMTDQFQDDYIERAVLPRTLEGR
                     LGHAAELSAALIFLASDAGSYVTGITLPVEGGMLTS"
     gene            complement(2599..5337)
                     /locus_tag="VFC01_24615"
     CDS             complement(2599..5337)
                     /locus_tag="VFC01_24615"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN82832.1"
                     /translation="MRPYGPLRGRDDELSTALAVVRRTRTHGASGVVLISGNAGVGKT
                     ALLSEICRQAAHLNIRLARSKCDEIEQASPGAPMVGLLRTGREPLLSAPEFEEISGLP
                     AEPLVLVDRIAGHLARLADGQRLLLAVDDVQWADRVSRYALRALISRLVGVPVVWLLV
                     SRSDDVGLAVSAADSVEVEHIRLGPLADSAIADIARDRLGPNLDARVEQMLDAAGGNA
                     FLALQIIDGMAQHAETDGMDGIPAQFRSAVRRRLTGLSGAARRLVEAAAVAGRPVSII
                     DLAELCEMNASPAHEEAVDDAVASGLLNSTGAELNFGHDLVKDAVYELIAPEVRRQLH
                     SRFARHFAGRVGDPALAAAHARAAATVGDIANARIMLDAAEALVTTSAVDAADLALQA
                     FRTLRPSQPQWLELGERAVCVLSRTQRTAEAIAVADALLATVDDVDVVGRIETYTIKP
                     MWLSGHSAALVARAERAIARAGARHDHVARFRAARALAHTRMLAADQATEGADAALAE
                     ARASRDHDALAFGLQAAGEAAHNERRHQLALKYFRELRSVTGVPYLAEEVMELQLLDR
                     YDDAQMLLDGATSDSGGKLGSMLPDLLFAQLKQDYNLGRLQEADDTASVLIELGQVTG
                     NTVHVVEGVLMRTTVALLRGESELAAQRLRPALSLADDDTIRHPGLALLSGWLTTEQG
                     DIDSGRRMLSELLETSQRTRSYWAWWPCFATVLFSIGLISGATNITERTVEFTEEGAR
                     RNPGVATLNGLALNLRGLLGGDVGTVAESVKILQSSPRPVLRAAGAEGYGRMLLTTGQ
                     REAALDHLDAAWDVYDRMGASAPRARVQRLMREAGARRAKWVGDHAHVDRVSLTEAER
                     RVAYLIANGHTNKATAKSLGISINTVGTHLRSIYSKLGVQSRVQLANVLRELGEIA"
     gene            complement(5334..5858)
                     /locus_tag="VFC01_24620"
     CDS             complement(5334..5858)
                     /locus_tag="VFC01_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82833.1"
                     /translation="MLPSSYDRQDCSLARALEIVGERWTLLVLRDAFYGVRRFNDFLA
                     HLDMPRTVLSNRLRSLIESGVMVKVPDPSHSGRWLYELTDEGMALWPALYALRSWGER
                     HSGRRPRAFTHDACGTQLDVTARCETCGVLPAPDHVFVTPVGEFTARTDPVALGLRRK
                     HRLLDFLPRDGDAA"
     gene            complement(6032..7021)
                     /locus_tag="VFC01_24625"
     CDS             complement(6032..7021)
                     /locus_tag="VFC01_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011208835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82834.1"
                     /translation="MIVCVLALDQVMGFELMIPGQVFGMANLAAGELGARRNGDPQGP
                     AEFEVRVCGQRSSISTTAAWGLVEIRTSYEMDALAEADLVVVPGTHRFLEEPDLQAVS
                     ALRAAADNGVRIAAMCVGAFTLAATGLLNGRRATTHWQWAGELARRYPEIDVDPSVLF
                     VDEGPVLTSAGVASGLDLCLHLLRQHAGADLAARTARRVVVPAWRDGGQAQYIEHVAP
                     ADSEHPLQPTIDWMEENALDALDLQAIANHASVSVRTLNRQFRDHFGTTPLGLLTRMR
                     VDRARRLLETTGLTVDRVAEQSGFGSYPSLRYHFLRTVGVSPHRYRQSYTAGR"
     gene            7094..7756
                     /locus_tag="VFC01_24630"
     CDS             7094..7756
                     /locus_tag="VFC01_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZN82835.1"
                     /translation="MSPPTGRIDLPDSEIACAAKQFAYDVSPAFVAHHSLRSYLYGRE
                     LAAAKGLKPDVDYDDELVFLSCMLHDLGVTEHANGDQRFEVDGADAAARFLRDHGVPE
                     ARVTTVWQAIALHTSVGLAHRFGAEQAITHLGIGADIVGNDKHLLPPGFADRVHEAWP
                     RHDLGYALTELIADQVEANPQKGPPLTFPGHLHQLMYPTTPTVTWFEVLGAAGWNDQP
                     RS"
     gene            7753..8397
                     /locus_tag="VFC01_24635"
     CDS             7753..8397
                     /locus_tag="VFC01_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011741203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZN82836.1"
                     /translation="MNIDMRMIPDLPRDPIASAALELARSTESKSVFNHSVRSYLWAV
                     LLAEHENATGEADYDPVLLFAATVMHDLGVGSGARGQQRFEVEGADLAAELLIAHGVS
                     TSDVDRVWEAIAIHTSAGIAERRGLLAYLTREGVGIDFGRQAKVVLNHQEAIHAHYPR
                     LAMVRSLVDAIVEHAGRSDAAAPRYSIPGELLHERRQHGVTRMEQAAAQSPWAD"
     gene            complement(8380..8529)
                     /locus_tag="VFC01_24640"
     CDS             complement(8380..8529)
                     /locus_tag="VFC01_24640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82837.1"
                     /translation="MTPTGVTFVAFHDPDGTDGSTRMWSPITSTLIMGRHDAFWSNRV
                     SVSPW"
     gene            complement(8549..8707)
                     /locus_tag="VFC01_24645"
     CDS             complement(8549..8707)
                     /locus_tag="VFC01_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82838.1"
                     /translation="MLFPIMIFLMVLSPLYIPIAVTVVHEFGNWRGNRVARPVGSVRQ
                     FRAQPAAA"
BASE COUNT         1634 a         2926 c         2787 g         1525 t
ORIGIN      
        1 cactggttca cagtgacgag ttcgcacgca tgacggcggt gatcgcccgc ggcaggcgac
       61 tggcgggtag tcgggccaat gcgatcaccg cgcgggtctg gcacctgatg aacctgccgg
      121 cgagtaccga tgtgcagcgg ctacgcatgc aggtcggcgt cttggaccgc gaaataaggc
      181 ggctgtccgt gcaactcgat cgcgattccc gatgagcgtc gtcgaacgcg tgcgccgcga
      241 ggtcgaacgc aacgcactgc gggcgcgaaa cggaatccgg ttggctgcag gtctccgacc
      301 gccggacgtc ggcctgacac ccaaggagat cgtgtggcgc aaaggtcgaa ctgaactctg
      361 gcgctaccgc aatgaacaga tccgggtctc tcctccgctg ctcatcgttt acagcctctt
      421 caaccgcagc tacatccttg acctgcagcc cggcaacagc ttcgtcgagc atctgctcaa
      481 cgccgggttc gacgtctatc tactggactt cggaattccc gacgagcgcg atgccggcaa
      541 cacgctcgac gactacgccg acgaatacct gccggccgcc gtcgaccgcg tatgccgggt
      601 ctctcacgac gagcaggtca acttgcttgg ctactgtttc ggcggggtgc tgtctctgct
      661 gtacgtcgct caccatctcg acgcgccact gcggagtctg actgctctga ccacaccggc
      721 agactttcag cgtctgggcc aattagggga tctgctcggt ggcgggctcg acattgagac
      781 cctgatcgac ggcgacggca atgtgcccgc ggacatcgtc ttgcagggat tccgcacgct
      841 gagaccgatc ggcgagatca cgcaatacgt cgacctgtgg gaacggctgt ggagcgatga
      901 gtacgtcgct gcgtatcagt cgatgaccgg gtgggctacc gaacaggttc ccctgccggg
      961 aggggtcgcc cgacaaatcg ttgacatggt gcgcgagaac ccgctgatga cagagcgatt
     1021 ggtggtcggc ggcgaccggg ttcagctgtc ggacatcacg gtgccgttcc tgcatgtgct
     1081 ggccagccgc gatcacatca tccccgaggc ggcggcggca ccgttgaccg gcctagtcgg
     1141 ctcgcgcgac aaacatgagc ttcggctcga tgccgggcac gtcgggctgt tggtcggcaa
     1201 gaccgccgcc aagaccacgc tcccgaccat catcgatttc ctcaaacagc gaagcagggt
     1261 gaaaccatga acatcgtcgc gtgcgctgca cagcacggcc gtaggctgac cgagctcttc
     1321 ggagagctgt ccgaacgaga tctgaccctc atcaaagaga acctcaccga tcccgtcgcg
     1381 gtacggcgat ggacggcgcg tcccggacag cagtgggtgg cgctcgacgg tactcacatc
     1441 gtcggctacg ccgcggtacg cccgctaccc gagtggtctg atcatgtggg cgagctgcgg
     1501 ctcgttgtcg atccggcgcg gcgtcgtgtc ggagtgggta gcgcacttgc tcgccacgcg
     1561 ctgatcgaag cggtgcgtgc cggacgtcgc aagctggtcg tggaactggc cgccgatcaa
     1621 gacaatgcgc tggcgatgtt cgcctcactg ggcttcaccg gcgaagcgct gttgcgcgac
     1681 cacatccggg atcgcaacgg ggaattccat gacctggtca tgctggcgca cttcgtcgac
     1741 gacacgtggg caggcatggg ctccatcggc ctgtcggacg aattgggcct ccctcaagct
     1801 gcttcagcta acggagccgt gcgatgacaa ttctcgaccg cttccgcatc gacgatcggg
     1861 tcgcgatcgt caccggggca tccagcgggt tgggcgtcgc cttcgccgcg gccctcgccg
     1921 aagcgggggc cgacgtgtca ctcggcgcgc gtcgcgagga ccgcctggcg gccacccgca
     1981 aacttgtcga agacgccggg cgacgggcca tcacagtgcg gaccgacgtg gccagtcccg
     2041 acgactgccg ggtactcgtc gacgccaccg tgcacgcctt cggcagggtc gacatcttgg
     2101 tcaacaacgc cggcatcggc acctccgtac cggccacccg ggaaaccccc gaacagtttc
     2161 gatcggtcat cgatgtcaac ctgaacgggt gttattggat ggctcaggca tgtgcccggg
     2221 tgatgcaacc cggaagcagc atcatcaaca tcgcgagcgt gctgggcttg accagtgccg
     2281 ggataccgca ggcggcgtat tccgcgtcga aagccgggct gatcggcctg acccgcgacc
     2341 tcgcccagca gtggaccggc cggaagggga tccgcgtcaa tgcgctggcg ccaggctatt
     2401 tcgagtccga aatgacggac cagtttcagg acgactacat cgagcgcgca gtgctgcccc
     2461 gcacgctgga aggcaggctc ggccatgctg cggagctcag cgcggcactg atcttcttgg
     2521 ccagcgacgc cggcagctat gtcaccggta tcacccttcc ggttgaaggc ggcatgctga
     2581 ccagctgagc tcacgctctc aggcgatctc acctagctca cgcaagacat ttgcgagttg
     2641 aacgcgagac tggacaccga gtttggagta gatcgaccgc agatgggtgc ccaccgtgtt
     2701 gatagatata ccaagcgatt tcgcggtcgc tttgttggtg tgcccgttcg cgatcagata
     2761 agccacccgc cgttcggcct cggtcaacga cacacgatcg acatgggcgt gatcgccgac
     2821 ccacttcgct cggcgggccc ccgcttccct catcagtcgc tgcactcgtg cgcgtggcgc
     2881 cgatgcaccc atgcggtcgt atacgtccca tgccgcgtcg aggtggtcga gcgcggcctc
     2941 acgttggccg gtcgtcagta gcatgcggcc atagccttcc gctccggcgg cccgcaagac
     3001 cggccggggg ctgctctgca ggatcttcac cgactcagcc accgtcccga cgtcgccgcc
     3061 gagcagtccg cgcagattca atgccaagcc gttgagcgtg gcgacaccgg gattgcgtcg
     3121 cgcaccctcc tcggtgaact cgacagtgcg ctcggtgata tttgttgcgc cactgatcaa
     3181 tccgatgctg aatagcacgg tcgcaaagca gggccaccat gcccaatagc tgcgcgtgcg
     3241 ttgggatgtt tccagcaact cggacagcat ccgccgcccg ctatcgatat cgccctgctc
     3301 ggtggtcaac catcccgaca acaaagccaa gccgggatgg cgaatcgtgt cgtcatcggc
     3361 aaggctcaac gccggtcgca accgttgcgc tgccaactcg gactcgccgc gcaacaacgc
     3421 cacagtcgtg cgcatcagga cgccctcaac gacgtgaacc gtgttgccag tcacctgtcc
     3481 gagctcgatg agcaccgacg cagtgtcatc ggcctcctgc agccgaccca ggttgtagtc
     3541 ctgtttgagt tgcgcaaaca acaggtccgg cagcattgaa cccagcttgc cgccgctgtc
     3601 gctggtcgcg ccgtcgagca gcatctgtgc atcgtcgtag cgatccagta actgcagctc
     3661 catgacctcc tcggccagat agggaacgcc tgtgaccgac cgcagctcac ggaaatactt
     3721 caacgccagc tgatgtcggc gttcgttgtg ggccgcttcg ccggcggcct gtagcccaaa
     3781 ggcgagcgca tcgtggtcgc gtgacgccct cgcctcagcc agagccgcgt cggccccttc
     3841 ggtggcttgg tccgcggcaa gcatccgggt gtgggccaac gcgcgtgccg cgcggaatcg
     3901 ggcaacgtgg tcgtgccgtg ctccggcccg ggctatggcg cgctcggctc gcgcgacgag
     3961 agcggccgaa tgaccggaaa gccacatggg cttgatggtg tacgtctcga tccggccgac
     4021 gacatcgacg tcgtcgacgg tcgcgaggag cgcgtcggcc actgcgatcg cttcggcggt
     4081 gcgttgagtg cgcgacagca cacagaccgc tcgttcacct agctcgagcc attgcggctg
     4141 gctaggccgc agagtccgaa aggcttgcag cgcaaggtcg gcggcgtcga cagcgctcgt
     4201 ggtgaccaat gcctcagcgg cgtcgagcat gatgcgggcg ttggcgatat caccgacggt
     4261 cgctgcggcg cgcgcatgcg cggcagccag cgccggatcg ccgaccctac ccgcaaaatg
     4321 gcgcgcgaag cgcgaatgga gctgacgccg gacctcggga gcgatcagct catagaccgc
     4381 gtccttcacc aggtcgtggc cgaaattcaa ttccgcgcca gttgaattga gcaggcccga
     4441 cgccaccgca tcgtcgacgg cttcctcatg cgcaggactt gcgttcattt cgcacagctc
     4501 ggccaaatcg atgatcgaaa ccggacgtcc ggcgactgcc gccgcttcga cgaggcgccg
     4561 cgccgcaccc gacagaccgg tcagccgtcg acgcacagcc gatctgaact gcgcgggaat
     4621 gccgtccata ccatcggtct cggcgtgctg tgccatcccg tcgatgatct ggagcgcgag
     4681 aaatgcattg ccgcctgccg cgtcgagcat ttgctccacg cgtgcatcga ggttggggcc
     4741 gaggcgatcc cgcgcgatat cggcgatggc cgagtcggcc agcgggccca gtcggatgtg
     4801 ttcgacttca acactatcgg cggcgctgac ggcaagaccg acgtcatcgg accggctgac
     4861 cagcagccac acgacgggaa cgccgacgag ccgagagatc aatgcccgca gggcataccg
     4921 gctgacgcga tcggcccact gcacgtcgtc gacggccagt aacaggcgct gcccatcagc
     4981 cagccgggcg agatggcccg cgatgcggtc gacgagcacc aaaggctcgg cgggcagccc
     5041 actgatctcc tcgaattcgg gcgccgatag gaggggttcg cggccggtgc gtaaaagccc
     5101 gaccatcggc gcccctggac tggcctgctc gatctcgtcg cacttgctcc gggccagtcg
     5161 gatgttcaag tgcgccgcct gacgacaaat ctcagacagc agcgcggtct ttccgacacc
     5221 ggcgttccct gagatcagca ccacaccgct ggcaccgtgc gtgcgcgtcc ggcggacgac
     5281 agccagcgcc gtggacagct cgtcgtctcg accgcgaagc ggcccgtacg gcctcatgcg
     5341 gcgtcgccgt ctcgtggcag gaaatcgagc aggcggtgct tgcgccgcag cccaagcgcc
     5401 acagggtcgg tacgcgccgt gaattcgccc accggcgtca cgaagacgtg atcgggcgcc
     5461 ggcaggaccc cgcacgtctc gcatctcgcc gtgacatcaa gctgtgtgcc acacgcatcg
     5521 tgggtgaacg cccttggccg ccgaccggag tgccgttccc cccacgagcg cagcgcgtac
     5581 aacgccggcc acaacgccat cccttcgtcg gtcagctcgt agagccaacg gcccgaatgg
     5641 ctgggatcgg gaaccttcac catcacgccg ctctcgatga gcgaccgtag gcggttgctc
     5701 aataccgtgc gaggcatgtc cagatgcgcg agaaagtcat tgaagcgccg gacgccgtag
     5761 aacgcatcac gcagcaccag gagcgtccac cgttcaccaa caatctcgag tgcccgcgcg
     5821 agtgagcagt cctgcctgtc gtacgagctc ggcagcatgt cgtcatcaaa tcagaaaccg
     5881 gccgatgagt ccagtgaatg gactcgtaat tgtgatttcc tccgacacgg catccacttt
     5941 cgctcgcttc gaagcacggc gacagcagtc aaatggccac aaaatggaaa gttctggcca
     6001 tcgcttcatg agtatcttca gctcctgctc ctcagcgtcc ggcggtgtag ctctgccggt
     6061 acctgtgtgg cgacaccccg acggtgcgga ggaagtgata ccgaagcgac gggtaggagc
     6121 cgaagccgga ctgctcggcg acgcggtcaa ctgtcaggcc ggtggtttcc agcagccggc
     6181 gtgcgcggtc gacgcgcatc ctggtgagca atcccaacgg ggtggtgccg aagtggtccc
     6241 ggaattggcg gttgagtgtt cgcacactca ccgaagcgtg gtttgcgatg gcctgtagat
     6301 cgagcgcgtc gagcgcgttc tcttccatcc agtcgatcgt cggttgaagc ggatgctcgg
     6361 agtcggcggg ggcgacgtgt tcgatgtact gcgcctggcc gccgtcgcgc cacgcgggta
     6421 ccaccacccg acgcgcagtg cgcgcggcca ggtcggcgcc cgcgtgttgg cggagcagat
     6481 gcagacacag gtccaggccc gacgccactc ccgccgacgt caacacggga ccttcgtcga
     6541 cgaacagcac actgggatcg acgtcgatct cgggataacg tcgcgctaac tcgccggccc
     6601 actgccaatg ggtggtcgcc cgcctgccat tcagcaggcc ggtagctgcc aacgtgaacg
     6661 ccccgacgca catagctgcg attcgcacac cgttgtcggc ggcggcacgc agcgccgaaa
     6721 ccgcctgcag atcaggctct tccaagaatc gatgcgttcc cggtaccacc actaggtcgg
     6781 cttccgcgag ggcgtccatt tcgtaggacg tgcgaatctc gaccaggccc cacgccgcgg
     6841 tcgtgctgat cgacgagcgc tgcccgcaca ctcgtacctc aaattccgcg ggaccctgag
     6901 gatcgccgtt gcggcgggct ccgagctccc ctgctgccag attggccatg ccgaagacct
     6961 ggccgggaat catcagttcg aaacccatga cctggtccag cgccaacaca caaacgatca
     7021 tgtggctaga tcttagcaac atatggctat ctggccactg gtatgcgcgg agtcgtcccg
     7081 gcacagttga ggtatgtcac ccccgaccgg tcgaatagat ctcccggact ccgagatcgc
     7141 ttgcgcggca aagcagttcg cgtacgacgt gtctcctgca ttcgtcgccc accacagcct
     7201 gcgcagctac ctttacggtc gcgaactggc ggcggccaag ggactgaaac ccgatgtcga
     7261 ctacgacgac gagctggtat tcctcagctg catgctgcac gaccttggcg tgaccgagca
     7321 cgccaacggt gatcaacggt tcgaagtcga cggcgccgat gccgctgctc gctttctgcg
     7381 ggaccacggg gtgccggaag cgcgggtcac caccgtctgg caggcgatag cgctgcacac
     7441 cagtgttggc ctggcccaca ggtttggcgc ggagcaagcc atcacccact tgggtatcgg
     7501 ggctgacatc gtcggcaacg acaaacacct gcttccgccg ggtttcgccg atcgcgtgca
     7561 tgaggcgtgg ccgcgccacg acctcggcta tgcgctgacc gaactcatcg cagatcaggt
     7621 ggaggccaac ccgcagaagg ggccccccct cacctttccg gggcatctcc accaactgat
     7681 gtacccaaca acgccaacgg tgacgtggtt cgaagtccta ggcgccgctg gctggaatga
     7741 tcagccgcgg tcgtgaacat tgacatgcgg atgatcccgg accttccgag agacccgatc
     7801 gcctctgctg cactggagtt ggcgagatcc accgagtcga agtcggtgtt caaccactcg
     7861 gtgcgcagct acctgtgggc ggtgctgctg gccgaacacg aaaacgccac cggcgaggcg
     7921 gactacgatc ccgtgttgct cttcgccgcg accgtcatgc acgaccttgg cgtcggcagc
     7981 ggcgctcgcg gccagcagcg attcgaggtc gagggcgcag accttgccgc ggaactgctt
     8041 atcgcgcacg gagtctcgac cagtgacgtc gatcgtgtgt gggaagcgat cgccatccac
     8101 accagtgccg gaatcgccga acgacgcgga ttgctcgcct atctcactcg cgagggagtc
     8161 ggtatcgact ttggtcgcca ggcaaaggtc gtgctcaatc accaagaagc gatccacgcg
     8221 cactatccgc gactggcgat ggtgcgttcc ctggtcgacg ccatcgtcga gcatgccggt
     8281 cgcagcgacg cagccgcacc ccgctactcc atcccaggcg agctcctgca cgaacgccgc
     8341 caacacggcg tgacccgcat ggaacaagcg gcagcacaat caccatgggc tgactgacac
     8401 gcggttcgac cagaacgcgt catgccgccc catgatcagc gtcgacgtga tcggcgacca
     8461 catccgggtg ctgccatcgg tgccgtcagg gtcgtgaaac gcgacgaacg tgacgccggt
     8521 tggcgtcacg ttcgtgagtg tatgcgtatt acgcggccgc cggctgcgcg cggaactggc
     8581 gaaccgagcc gaccggacga gcgacacgat tgccgcgcca gttgccgaac tcgtgaacca
     8641 ccgtgacggc gatcgggatg tacagcgggg acagcaccat caggaagatc atgatcggga
     8701 agagcattgg atctctcgca ttctccgcgg gcggaatcgt tggccttttg tcttggggaa
     8761 ctcatcgctg agttcgtgat gagaaaccta cgagcgaacc cccagctcag cgatgtcccg
     8821 caacgaccaa cttctgacgc tgtcttatct cttggaaaca aactgctcag at
//
LOCUS       DASRJA010000557         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2692717,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000557 DASRJA010000000
VERSION     DASRJA010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2692717"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VFC01_24650"
     CDS             <1..368
                     /locus_tag="VFC01_24650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZN82839.1"
                     /translation="TEGLAVLQCGDPTGQGTGGPGYQFENEYPTNQFQPDDPKLQEPV
                     KYPRGTLAMANAGPGTNGSQFFLVYKDSELPPNYTVFGTIDQTGLATLDKIAAAGTAD
                     GGQDGKPKNDVQIKSIQLD"
     gene            376..1389
                     /locus_tag="VFC01_24655"
     CDS             376..1389
                     /locus_tag="VFC01_24655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZN82840.1"
                     /translation="MTTPPPPYGAYPPPPGPYPGYYGYPQYAQPQSTNALAIVSLVCA
                     FLFAPLGIIFGHISLSQIRKTGEEGRGLAIAGLVISYLITVLTIVVVVLSVVFVVQVA
                     RDLESMDGLTPEYPDVTAFPSPPANNLPPFDPPKTLGSNCQYPATTEPPSKPVKPPQN
                     GRIATDPALIRASIATSQGTLGLELDNSMTPCTINNFTSLAKQGFFDNTTCHRLTTTP
                     ELGVLQCGDPTGTGTGGPGYRFPNEYPTNQYRLADPALKIPVLYPRGTLAMANSGPGT
                     NGSQFFLVYKDSQLPPTYTVFGTIDSAGLTTLDTVAAAGVSGGSDDGKPIVDVTITSV
                     QIG"
     gene            complement(1400..1693)
                     /locus_tag="VFC01_24660"
     CDS             complement(1400..1693)
                     /locus_tag="VFC01_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HZN82841.1"
                     /translation="MRTALVVVIASLAMVLSGCGSTIKPEGAAGSVTDLVSKQTGFKP
                     NDVKCPSGVDAKEGTEFECHFTGPEGKEYTAHMKVTKVEGDDVQFYIKTEPSK"
     gene            complement(1745..>2526)
                     /locus_tag="VFC01_24665"
     CDS             complement(1745..>2526)
                     /locus_tag="VFC01_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZN82842.1"
                     /translation="AKAKIRQWFAKERREEALESGKDAIAREVRRSGIPLQRLMNAES
                     MAALARELRYTDVSALYTAVGEGHVSARHVVQRLVAQLGGDEEAADELAERSTPATMP
                     VRQRSTDDVGVSVPGAPGVLTKLAKCCTPVPGDNIMGFVTRGGGVSVHRTDCTNAASL
                     QAQSERIIDVQWAPSPSSVFLVAIQVEALDRHRLLSDVTRVLADEKVNILSASVTTSD
                     DRVAISRFTFEMGDPKHLGHLLNVVRNVEGVYDVYRVTSAA"
BASE COUNT          458 a          873 c          774 g          421 t
ORIGIN      
        1 ccactgaggg cctggccgtg ttgcagtgcg gcgacccgac gggccagggc accggcggac
       61 cgggctacca gttcgagaac gagtacccga cgaaccagtt ccagcccgac gatccgaagc
      121 tgcaggagcc ggtgaagtat ccgcggggca cgctcgcgat ggccaacgcc gggcccggca
      181 ccaacggcag ccagttcttc ctcgtgtaca aggattcgga gctgccgccg aattacacgg
      241 tgttcggcac gatcgaccag accggcctgg cgacgctgga caagatcgca gctgcaggca
      301 ccgccgacgg cggccaggac ggtaagccga agaacgacgt gcaaatcaag tcgatccagc
      361 tcgactagac cggccatgac caccccgccc ccgccgtacg gcgcctaccc gccaccgcca
      421 ggcccgtacc cggggtacta cggctatccg caatatgccc agccgcagtc gaccaatgcg
      481 ctggccatcg tgtcgttggt gtgcgcgttc ctgttcgcgc cgctgggcat catcttcggc
      541 cacatctcgc tgtcgcagat ccgcaagacc ggcgaggagg gccgcgggct ggcaatcgcg
      601 gggttggtga tcagctacct gatcacggtc ctcacgatcg tggtggtggt gctcagcgtc
      661 gtgttcgtcg tccaggtcgc gcgtgacctc gagtccatgg acggcctgac cccggagtac
      721 ccggacgtca cggcgttccc atcgccgccg gccaacaact tgccgccgtt cgacccgccg
      781 aagacgttgg gctccaattg ccaatatccg gcgacgacgg aaccgccgag caagcccgtc
      841 aagccgccgc agaacggtcg gatcgcgacc gatccggccc tcatccgcgc gagcatcgcg
      901 accagtcagg gcacgctcgg actggagttg gacaacagca tgacgccatg cacgatcaac
      961 aacttcacca gcctggccaa gcaggggttc ttcgacaaca ccacgtgcca ccggctgacc
     1021 accacacccg aactcggtgt gctgcagtgc ggcgatccga ccggcaccgg cacgggcggt
     1081 ccgggttatc ggttcccgaa cgaatatccg accaaccagt accggctggc ggatcccgcg
     1141 ctgaagattc cggtgctgta cccgcgcggc acacttgcga tggcgaactc cgggcccggc
     1201 accaacggca gccaattctt cttggtgtac aaggattctc agctgccgcc gacctacacg
     1261 gtgttcggca ccatcgacag cgccggactg acgacgttgg acacggtcgc cgcggcgggg
     1321 gtttccggcg gaagcgacga cggcaagccc attgtcgacg tgacgatcac gtcagtccag
     1381 atcggctagg aacagcgcac tatttgctcg gttcggtctt gatgtagaac tgaacgtcgt
     1441 cgccctcgac cttggtgacc ttcatgtgag cggtgtattc cttgccctca ggcccggtga
     1501 agtggcattc gaactcggtg ccttccttcg cgtcgacgcc cgacgggcac ttgacgtcat
     1561 tgggcttgaa gccggtctgt ttggacacca ggtcggtgac ggaccccgcc gctccctcgg
     1621 gcttgatcgt ggaaccacaa ccggacaaca ccatcgccag cgaagcgatt acaaccacca
     1681 atgcagtgcg catcgccgca gtattccaga catcgcaaat gccgaagcgc atttaagcgg
     1741 acgttcacgc cgcggatgtc acgcggtaga cgtcgtagac gccctccacg ttgcgcacca
     1801 cgttcagcag gtgacccaaa tgcttcggat cacccatctc gaaggtgaac cggctgatcg
     1861 ccacccggtc gtcggacgtc gtcaccgacg cggacaggat gttgaccttc tcgtcggcca
     1921 acacccgggt gacgtccgag agcagccggt gccgatccag cgcttcgact tggatggcca
     1981 ccaggaacac agacgacggc gacggagccc actgcacgtc gatgattcgc tccgactgtg
     2041 cttgcagcga cgcggcgttc gtgcagtcgg tgcggtgcac gctcacacct ccgccgcggg
     2101 tgacgaaccc catgatgttg tcgccgggta ccggcgtgca gcactttgcg agcttcgtca
     2161 gcacgccggg agcgccgggc accgagaccc cgacgtcgtc ggtgctgcgc tgacgcaccg
     2221 gcatggtcgc tggtgtcgag cgttcggcga gctcgtcggc ggcttcctcg tcaccgccga
     2281 gctgggccac cagtcgctgc accacgtgcc gcgcggagac gtgaccctcg cccaccgccg
     2341 tgtagagcgc cgacacatcc gtgtagcgca gttctcgggc cagcgccgcc atcgactcgg
     2401 cattcatcaa gcgctgcaag ggaattccgc tgcgacgcac ctcgcgtgcg atcgcgtcct
     2461 taccggattc cagcgcctct tcgcgacgct ccttggcgaa ccattgccgg atcttcgcct
     2521 tcgcgc
//
LOCUS       DASRJA010000558         6726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1509991,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000558 DASRJA010000000
VERSION     DASRJA010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509991"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1302
                     /locus_tag="VFC01_24670"
     CDS             130..1302
                     /locus_tag="VFC01_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HZN82843.1"
                     /translation="MNSNIRGAAAVVGVADEVSPSGVIDVPLRELEARVITSALSDAG
                     LALSDVDGLCTCTGGTLMHSVELAEYLGITPRFTDATQTGGASYGLYLEHAAAAIAAG
                     LAETVVIVYASTPRASRKRGEKGLGVFATPERLEWETPFGVMLPISAYALAANRHMAA
                     YGTTAEQLAQIAVDTRSWATRNPRAHLRDPITVDDVLGSGFLAEPVHKLECCLVTDGA
                     AAVVVTSAERARDLARPPAYVLGAASAASHAMITQMPDLTITPGAMSGPAAFEGAGVT
                     PSQIDVVQLYDSFTITVLLALEDLGFCKKGEGGPFVADGVLAPGGDLPGQTTGGGLAY
                     THPGAFGAFLLVEATRQLRGDCGERQVANAQTALAHGTGGVLSATSTVILGTEATL"
     gene            1299..1694
                     /locus_tag="VFC01_24675"
     CDS             1299..1694
                     /locus_tag="VFC01_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZN82844.1"
                     /translation="MSETFPVPAPSAVSAPFWAATEKHQLMMQRCESCHRLVWYPRAL
                     CPRCGEFSLRWEQLSGRGVVYAVSVHHRAAHPAFADRVPYSVVLVDLEEGVRMMSNVF
                     GTPPAVGDAVSLGWVALDDGRNLPIFEAP"
     gene            1691..2086
                     /locus_tag="VFC01_24680"
     CDS             1691..2086
                     /locus_tag="VFC01_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN82845.1"
                     /translation="MSAASDLRRIREEIVLRHVAGENDRDLEAVMATFTRPRYEIVPS
                     AMVYDGDEAVREMILRQWDELPWMQYTAEGIYHGPDGLIVETRTTCPGTPIDMFSVNL
                     FGFDGTGLVLERCYFDRMLFAAQLELLND"
     gene            2144..4165
                     /locus_tag="VFC01_24685"
     CDS             2144..4165
                     /locus_tag="VFC01_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HZN82846.1"
                     /translation="MATQLDTEAQSSTSDRAVELYRLMSKVHHADQRVRKGLSSGEIA
                     MSYWPVDGQEAMSAGAALALSDADQLVTTYRGLGDVVAKGIDLGGYFGEILGRETGLS
                     RGKAGAMGVFDPAHGIAWTTGIVGAGPLIANGIALAASIRNTGQVVLVSFGDGATSIG
                     YVHEAMNMAALWSLPVVFFCQNNAWAECTAMSRYTRTTKLSDRASGYAMLGVTIDGTD
                     PHEVYAAVSEAAERARSGGGPSFVEAVAYRLQGHYFGDGMGYADPDELAAKRADPPFA
                     RYRRRLVDEGITAEAELDRIDAEVSAEIEDAFSQARDAAPPDVVELTRDVFSGNGTQF
                     SVRGQVHAAVPDGETEDLGLVQAIHRTLDRAMESDDSIILLGEDIGDPSGGGMFKVTA
                     GLSAKYGENRVLDTPIAESSIIGAAIGASLAGLRPVAELMFMDFLGVAMDQIANHAAK
                     VRYMSGGRKGAPLVIRTMVGMAAGPQHSQAFEAWAMHTPGIKVVWPSTAADAAGLLNA
                     CLREDDPCLFVESMKLYYGGGKGPVPVADYVIPLGQADVKRPGSDVTICTYGVMVHAA
                     LEAAKELDSEGVSVEVVDLRTLVPLDLETVLDSVRKTKRLVVAHESVGFCGPGAEIAA
                     AVGTELFGVLAAPIQRVAGTYTPVPRAATLEAACRPGAAQLIEAIRRIV"
     gene            4162..4389
                     /locus_tag="VFC01_24690"
     CDS             4162..4389
                     /locus_tag="VFC01_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZN82847.1"
                     /translation="MTEIRMPKPGDAITEAEVTEFYVAEGQSAVEGDPLYAIATDKVE
                     MDIEAPASGVVTWRVEVGGTYDVGELIAVIS"
     gene            4386..5231
                     /locus_tag="VFC01_24695"
     CDS             4386..5231
                     /locus_tag="VFC01_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN82848.1"
                     /translation="MTGLPDSLKIDLGAPELIGEIVGDTLTVTINRPEKRNAVTADGY
                     HGIKRAAMIVADEPGLQFLVITGSDDVFCAGGDMNSVSNPDRSWDAFTEAYDATPFEV
                     LGKIPKIVVCAVNGIAMGGGLVMTLFADLVIASDRARLRVPDLTRGVYEAFVAARIPQ
                     RIGTLRANHMIYGNDWVDAVEAERLGLVGKIVPHEALASETQTLLDRVRKTGPAARAA
                     MKREMARALPAVDVSGYWSSIGTSEQIEAFSAFLEKRSPRWSVNSDRDAFVHTRRPSW
                     MPSGS"
     gene            5247..6218
                     /locus_tag="VFC01_24700"
     CDS             5247..6218
                     /locus_tag="VFC01_24700"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HZN82849.1"
                     /translation="MITTRFTESFGIEQPIVQGGMQWVGRAELAAAVSDAGGLGLITA
                     LTQPTPADLANEIARARDLTDKPFGVNLTILPTIDPPPYDEYRRVIVDAGIKIVETAG
                     SNPAPHLPMFHDNGIKVLHKCTSVRHAVKAQSLGVDGISIDGFECAGHPGEDDIPGLV
                     LIPAAAKEIEIPMIASGGFADARGLVAALALGADGINMGSRFMCTVESPIHQKVKDAI
                     VAGSELDTELIFRTLRNTGRVASNTVSREVVEILNEGGQFEDIAHLVAGKRGVKVYET
                     GDLDAGIWWVGTAMGLIDDIPTAGELVSRIVTEAEQIITERLCGAMR"
     gene            6215..>6726
                     /locus_tag="VFC01_24705"
     CDS             6215..>6726
                     /locus_tag="VFC01_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN82850.1"
                     /translation="MTELPAGTSKLTGCDEILVADDGPVRIVTLNNPGDANAVNNAMH
                     GAFARLWTTLGEDPDARAVLITGSGDYFSAGGNLVEWLETHVNNPVTRREGMRDARRI
                     VRDMIDFPLPIVAAVNGPAVGFGCSIAVLCDITLMSDRAFFADTHVASGLVAGDGGSV
                     LWPLMMSLMKA"
BASE COUNT         1215 a         2068 c         2253 g         1190 t
ORIGIN      
        1 ctatgcgcgc ggacgcctca cccgatacca agtgcccgcg aagctgttga tcgtcgacga
       61 gctgccgaga accccgtcgt tgaaggtcag tcaatccgaa ctgcgcgaac tgttcgaaca
      121 ggagcggaag tgaattccaa catccgaggt gccgccgccg tggtcggtgt cgccgacgaa
      181 gtgtcacctt cgggcgtgat cgatgtaccg ctgcgggagc tcgaggcgcg cgtcatcacc
      241 tccgcgctat cggatgccgg cctggctctc agtgatgtcg acggcctgtg cacatgtacc
      301 ggcggcacct tgatgcactc cgtcgaactt gccgaatacc tgggcattac accgagattc
      361 accgatgcca cgcaaaccgg tggtgcgagc tacgggttgt acctcgaaca tgccgcggcg
      421 gccatcgccg cgggtctcgc agaaaccgtg gtcatcgtct acgcctccac gccacgtgcc
      481 tcgcgcaaac gcggcgaaaa aggactcggt gtcttcgcca cacccgagcg gctggaatgg
      541 gaaacaccgt tcggcgtgat gctgccgatc agtgcctacg cacttgccgc caatcgacac
      601 atggcggcct acggcaccac cgcagagcaa ctcgcgcaga tcgcggttga cacgaggagc
      661 tgggccaccc gcaatccgcg tgcgcacctg cgcgacccga tcaccgtcga cgacgtgctt
      721 ggctcgggct tcctcgccga gccggtccac aagcttgaat gctgtctcgt caccgatggc
      781 gccgccgcgg ttgtcgtcac cagcgccgaa cgggcccgcg atctcgcgcg gcctcctgcg
      841 tatgtgctgg gggcggcgtc ggcggcatcg cacgcaatga tcacgcagat gccggatctc
      901 accatcacgc ccggcgccat gtcgggacct gccgcattcg aaggtgctgg cgtgactccg
      961 agccagatcg acgtcgtgca gttgtacgac tctttcacca tcaccgtgct gctcgcgttg
     1021 gaagacctcg gcttctgcaa aaagggcgaa ggagggccgt tcgtcgcgga cggagtactt
     1081 gcgccgggcg gtgaccttcc cggccagacc accgggggtg gactcgcgta cacgcatccc
     1141 ggtgcgttcg gggccttcct gctcgtcgag gccacccggc aattgcgcgg cgactgcggc
     1201 gaacgacagg tggcaaatgc tcagacagcc ctggcgcacg gaaccggggg cgtgctctcg
     1261 gcgacgtcga cggtgatcct gggaacggag gccaccctat gagcgagacg ttcccggtgc
     1321 cggcgcccag tgcggtgtcc gcgccgtttt gggcggcgac cgagaagcat cagctgatga
     1381 tgcagcgctg cgaatcatgc catcggttgg tctggtatcc gcgcgcgctg tgtccgcggt
     1441 gtggcgagtt ctcgttgcgc tgggagcagc tgtcgggccg gggtgtggtc tacgcggtca
     1501 gcgtgcacca ccgcgccgcc catcctgcgt tcgccgaccg ggtgccctac tcggttgtgc
     1561 tggtggacct cgaagagggc gtgcggatga tgtcgaacgt gttcggcaca ccgccagcgg
     1621 tcggcgacgc ggtatcgctg gggtgggtcg cgctggacga cggacgcaac ctcccgatct
     1681 tcgaggcacc atgagcgccg caagcgacct gcggcgaatc cgcgaggaaa tcgtgctgcg
     1741 ccacgtcgcg ggggagaacg accgcgatct ggaagccgtg atggcgacgt tcacgcgtcc
     1801 gcgttacgaa atcgtaccga gcgcaatggt ttacgacggc gacgaagcgg tgcgggagat
     1861 gatattgcgg caatgggacg agttgccgtg gatgcagtac accgcggagg gcatctacca
     1921 cggcccggac ggtttgatcg tggagaccag gacgacctgt ccggggacac cgatcgacat
     1981 gttcagcgtg aacctgttcg gtttcgacgg cactggactg gtcctcgagc gctgctactt
     2041 cgaccggatg ctgttcgcgg cacagctgga gctattgaac gattagacaa aattcgtcat
     2101 gttgtagaat cggtccgccg aagtgcacgg gcaaggagtg tgtatggcca cgcagttgga
     2161 caccgaggcg cagtcatcga ccagcgatcg cgccgtcgag ttgtaccgcc tgatgagcaa
     2221 ggttcaccac gctgatcaac gagtccgaaa aggcttgtcg tcgggtgaga tcgcgatgag
     2281 ttactggccg gtggacggtc aggaggcgat gtcggcaggt gcggccctgg cgctatctga
     2341 cgctgatcaa ctcgtcacta cctaccgagg gctgggtgac gtcgtcgcca agggcatcga
     2401 cctgggcggg tacttcggcg agatcctcgg tcgtgagacc ggcctgtcga ggggaaaggc
     2461 cggcgccatg ggcgtcttcg atcctgcgca tgggatcgca tggaccacgg gcatcgtcgg
     2521 cgcgggcccg ctcatcgcga atggcatcgc gctggccgcg tcgatccgca ataccgggca
     2581 agtggtcctc gtcagcttcg gggacggtgc gaccagcatc ggctacgtcc acgaagcaat
     2641 gaatatggcg gcgctgtggt cgcttccggt ggtgttcttc tgccagaaca acgcgtgggc
     2701 cgagtgcact gcgatgtcga ggtatacgcg cacgacgaaa ctttccgatc gcgcgagcgg
     2761 ctacgcgatg ctcggcgtga cgattgacgg cacggacccg cacgaggtgt acgccgcggt
     2821 gtcggaggcc gccgaacgcg ctcgctcggg cggcggtccg tcgtttgtcg aagcggtcgc
     2881 ctaccggttg cagggccact acttcggtga cgggatgggc tatgccgatc ccgacgagct
     2941 cgcggccaag cgtgccgacc cgccgttcgc gcgctatcga cgccgtttgg tcgacgaagg
     3001 aatcacagcc gaggcagaac tcgaccggat cgatgccgag gtgtcggcag agatcgagga
     3061 cgcgttctcc caggcccgcg atgccgcgcc gcccgacgtg gttgaactca cccgtgatgt
     3121 cttctccggc aacggaaccc aattttccgt gcgtgggcag gtgcatgcgg ccgttcccga
     3181 tggcgaaacc gaggatctcg gcctcgtgca agccatccat cgcactctcg accgggcgat
     3241 ggagtccgac gactcgatca tcctgctggg tgaggacatc ggtgacccgt cgggcggagg
     3301 gatgttcaag gtcactgcgg gtctttcggc gaagtacggt gagaaccggg tgttggacac
     3361 tccaattgca gagtcatcga tcatcggggc cgcgatcggc gcctcgctgg cgggattgcg
     3421 ccccgtcgcc gaactcatgt tcatggactt cctcggggtg gccatggatc agatcgccaa
     3481 tcacgccgcg aaggtccgct acatgtccgg cggccgtaag ggcgcgccgc tggtcatccg
     3541 cacgatggtg ggaatggctg cggggcctca acattcgcag gcttttgagg cgtgggcgat
     3601 gcacaccccg ggcatcaagg tggtgtggcc gagcaccgcg gccgatgcgg cagggctgct
     3661 caatgcatgc ctgcgcgaag acgacccatg tctgttcgtc gaatccatga agctctacta
     3721 cggtggcggc aaagggccgg tccccgtggc cgattacgtc attccgcttg ggcaggccga
     3781 cgtgaaacgg ccgggcagtg acgtgaccat ctgcacctat ggcgtgatgg tgcacgcagc
     3841 actcgaagcg gcgaaagaac tcgacagcga aggagtttca gtagaggttg tcgacctccg
     3901 cacactcgtg ccgcttgacc tcgaaaccgt tttggattca gtccgcaaaa ccaagcgcct
     3961 tgtcgtcgct cacgagtcgg tcggcttctg tggacctggc gccgagatcg cggccgccgt
     4021 cggcaccgaa ctgttcgggg tcctggcggc gccgatccaa cgggtggcag ggacctacac
     4081 gccggtaccg cgcgccgcga ccttggaggc cgcgtgccgc ccgggcgcgg cgcagctcat
     4141 cgaggcaatc aggagaatcg tgtgacagag attcggatgc ccaaaccagg ggacgccatc
     4201 accgaagctg aggtgacgga gttctatgta gccgagggcc agtccgcggt cgagggcgac
     4261 ccgctgtacg cgatcgccac cgacaaggtc gaaatggata tcgaggcgcc ggcatccggt
     4321 gtggtcacgt ggagggtcga ggtggggggc acctacgacg tcggcgaact catcgcggtg
     4381 atctcgtgac cggattgccc gactccttga aaatcgatct cggtgcaccc gagttgatcg
     4441 gtgaaatcgt cggcgacaca ctcacagtca ccatcaaccg gccggagaag cgcaacgcgg
     4501 tgaccgctga cggctatcac ggcatcaagc gcgcagcgat gatcgtggcg gacgaaccag
     4561 gtctgcagtt cctcgtgatc accggatccg acgacgtctt ctgtgccggc ggcgacatga
     4621 actcggtgag caatcccgat cgttcctggg acgcgttcac ggaggcgtac gacgccactc
     4681 cgtttgaagt gctcggcaaa atcccgaaaa tcgtcgtgtg cgcggtcaac ggcatcgcga
     4741 tgggcggggg cctggtgatg acactgttcg ccgatctggt gatcgcctcg gatagggccc
     4801 ggctgcgcgt ccccgatctg acccgcggcg tgtacgaggc atttgttgcg gcacgaatcc
     4861 cgcaacgcat cggtacgttg cgcgccaacc acatgatcta tgggaatgat tgggtcgacg
     4921 ctgtcgaggc cgaaaggctc ggactggtcg gcaagatcgt cccgcacgag gcccttgctt
     4981 cagaaacaca aacgctgctg gatcgggtgc gcaagaccgg cccggccgca cgcgcggcga
     5041 tgaagcggga gatggcacgc gctcttcccg ccgtcgacgt cagcggttac tggtcgtcga
     5101 tcggaacgtc cgaacaaatc gaggcgttct ctgccttcct cgagaagcgt tcgccgcgtt
     5161 ggtcggtcaa ctcagatcgc gacgcattcg tccatacccg taggccgtca tggatgccgt
     5221 ctggctcctg aaagggattg actgctttga taacaacgag gttcaccgaa tcgttcggca
     5281 tcgagcagcc cattgtgcag ggtggaatgc aatgggtggg ccgtgcggaa ctggcggcgg
     5341 ctgtttcgga cgcaggcggg ctcgggctca tcacggcgct gacgcagccg actccggcgg
     5401 acctggccaa cgagatcgcg cgtgcccgcg acctcaccga caagccgttc ggtgtgaact
     5461 tgacgatcct gccgaccatc gacccgccac cttatgacga ataccgccgg gtgatcgtcg
     5521 acgcgggcat caagatcgtc gagacggcgg ggtccaaccc ggccccgcat ctgccgatgt
     5581 tccacgacaa cgggatcaag gtgctgcaca agtgcacctc ggtgcgtcac gcggtcaagg
     5641 cgcagagcct gggtgtagac ggcatcagca tcgacgggtt tgagtgcgcg ggccacccgg
     5701 gcgaggacga tatccctggc ctcgtcttga ttcccgccgc tgccaaggaa atcgagatcc
     5761 cgatgatcgc gtcgggcggc ttcgccgacg cccgcggtct ggtcgctgcg ctggcgctgg
     5821 gcgcagacgg catcaacatg ggttcgcgct tcatgtgcac ggtcgagtcc ccgattcacc
     5881 agaaggtcaa ggacgccatc gtggccggca gcgagctcga caccgagctg atcttccgaa
     5941 cgctacgcaa cacgggccgc gtcgcaagca acaccgtgtc gcgagaggtt gtcgaaatcc
     6001 tcaatgaagg tggtcaattc gaggacatcg ctcatttggt cgccggcaag cgtggtgtga
     6061 aggtgtacga aaccggcgac ctcgatgccg gaatctggtg ggtggggacg gcaatgggat
     6121 tgatcgacga catcccgaca gcgggcgaac tcgtgtcacg gatcgtgacc gaggccgaac
     6181 agatcatcac cgaacggctc tgcggagcaa tgcgatgacc gagttaccag cggggacttc
     6241 caagctgacc ggctgcgacg agatcctggt agccgacgac ggtcctgtcc ggatcgtgac
     6301 gttgaacaac cccggcgacg ccaacgcggt caacaatgcg atgcacggcg cgttcgcgcg
     6361 gctgtggacg acactcggcg aggatcccga cgctcgggcg gtgctcatca ccggcagcgg
     6421 agactatttc tcggctggcg gaaatctcgt ggagtggctg gagacgcacg tcaacaatcc
     6481 ggtcacgcga cgtgaaggta tgcgagatgc ccgccgcatc gtgcgcgaca tgatcgactt
     6541 cccgctaccg atcgtcgccg cggtgaacgg ccctgcggtc ggattcggtt gcagcattgc
     6601 ggttctgtgc gacatcacgc tgatgtcaga ccgcgcgttc ttcgccgata cccatgtcgc
     6661 tagcggcctc gttgccggcg acgggggatc cgtgctctgg ccgctgatga tgagcctgat
     6721 gaaggc
//
LOCUS       DASRJA010000559         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1718922,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000559 DASRJA010000000
VERSION     DASRJA010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718922"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VFC01_24710"
     CDS             <1..675
                     /locus_tag="VFC01_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZN82851.1"
                     /translation="RMRTRGDVDMPVTIRIPSFGGIGAVEHHSESTESYWLHTAGLKV
                     VVPSTPSDAYWLLRQAIVSRDPVVYLEPKRRYWTREAVDTNTPALPIGRAAVRRVGAD
                     VTVVTYGSLVATALSAADLAEEHGWSLEVVDLRSLNPLDFDTVADSVRKTGRAVVMHE
                     GPRTLGFGAELAARIQEELFYDLEAPVLRATGFDTPYPPARLEKLWLPGPDRLLDCVE
                     RAMEKP"
     gene            672..1862
                     /locus_tag="VFC01_24715"
     CDS             672..1862
                     /locus_tag="VFC01_24715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZN82852.1"
                     /translation="MSAVKEFLVPDLGEGLEDATITGWSVAVGDDVELNQTLCTVETN
                     KAEVEIPSPYAGRIVELGGAEGDTLNVGTMLVRIATDDLTPVESENGTPKRKPVLVGY
                     GADDDMDTSRRASVGERPRAKPPVRKLAAELNVDLSGVTGSGPDGVITREDVLAAAGR
                     SAPTPEMVAVRGVQAEMAHRMTLSRRQIPDAHAGVQVDCSSLIQMRDRLRDSTDDESP
                     ITPFVLTLRLLTIALRHHPNLNSTWVDTTDGPQIHLHSAVHLGFGVAAPRGLLVPVVS
                     DAQDKTTRELATVIARLIRDARAGTLKPSELQGSTFTVSNFGALGLDEGVPVINYPEA
                     AILGMGSLKPRPAVVDNEVVVRPTMSLTCAFDHRIVDGAQVAAFLRELRDLVEAPELA
                     LLDL"
     gene            complement(1863..2126)
                     /locus_tag="VFC01_24720"
     CDS             complement(1863..2126)
                     /locus_tag="VFC01_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82853.1"
                     /translation="MKKLAAVLMTAAAAAAVVPVAVAPVAQADICAGAHGRHVGVGGC
                     TNIAGDVATGVAIAAATDPYVPGEIPCYTVEGVPYYTPPGDPC"
     gene            complement(2187..>2652)
                     /locus_tag="VFC01_24725"
     CDS             complement(2187..>2652)
                     /locus_tag="VFC01_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN82854.1"
                     /translation="AVNGAAVGAGLNLALAADVRIAGPAALFDPRFQKLGIHPGGGAT
                     WMLQRAVGPQVARASLLFGMRFDAEAAVRYGLALQIADDPVAAARELAAGPAAAPRGV
                     VIATKASMRATANPGVIDTEQHRIAVDTELGPQAASITSPEFARRLAAARRK"
BASE COUNT          424 a          871 c          925 g          432 t
ORIGIN      
        1 cggatgcgca cccgcggcga cgtcgacatg ccggtgacca tccgcattcc gtcgttcggc
       61 gggatcggcg cggtggaaca tcattcggag tccaccgaga gctactggct gcacaccgca
      121 ggcctcaagg tcgtggtgcc gtcgacgccg tcggatgcgt attggctact gcggcaggcg
      181 attgtcagcc gggaccccgt cgtctacctc gaacccaaac ggcggtactg gacccgcgag
      241 gccgtcgaca ccaacacgcc cgcactgccg atcggacgcg cagcggttcg tcgcgtcggc
      301 gcggatgtca cggtggtgac gtacgggtcg ctggtcgcca cggcgctcag cgccgccgac
      361 cttgccgaag agcacggctg gagcctcgaa gtcgtcgacc tgcggtcgtt gaacccgctc
      421 gacttcgaca ccgtcgccga ctcggtgcgc aagaccggcc gcgcggtggt gatgcacgaa
      481 ggcccgcgca cgttgggctt cggcgccgag ctggccgcac gcatccagga ggagttgttc
      541 tacgacttgg aggcaccggt gttgcgcgcc acagggtttg acacgccgta tccaccggcc
      601 cggctggaga agttgtggct accgggcccg gaccggctgt tggactgtgt cgagcgggca
      661 atggaaaagc cgtgagcgcc gtaaaggagt tcctcgttcc tgacttgggc gaaggtctcg
      721 aagacgcgac gatcaccggc tggagcgtgg cggtcggtga cgacgtcgaa ctgaatcaga
      781 cgctgtgcac ggtggagacc aacaaggccg aagtcgaaat ccccagcccg tatgcggggc
      841 gcatcgtcga actcggaggc gccgagggcg acacgctgaa cgtcggcacg atgttggttc
      901 gcattgccac cgatgacttg actccggtgg agtcggagaa cggcaccccc aagcgcaagc
      961 cggttctcgt cggctacggt gccgacgacg acatggacac cagcaggcgg gcgtcggtcg
     1021 gtgaacggcc ccgggccaag ccgccggtgc ggaagctggc ggccgagttg aacgtcgacc
     1081 tgtccggtgt gaccggttcc ggcccggatg gtgtgatcac tcgcgaggat gtcctcgctg
     1141 cggcgggcag gtctgcgcca acccccgaga tggtcgcggt gcgcggtgtg caggcggaga
     1201 tggcgcaccg aatgacgctg tcgcgcaggc agatacccga tgcgcacgcc ggtgttcagg
     1261 tcgactgttc gagcctgatc cagatgcgcg atcgcttgcg cgacagcacg gacgacgagt
     1321 cgccgatcac gccgttcgtg ctgaccttgc ggctgttgac gatcgcgctg cgacaccacc
     1381 ccaacctgaa ttcgacatgg gtggacacca ccgacgggcc acagatccac ttgcactccg
     1441 ccgtgcatct gggtttcggc gtggcggcgc cacgcggact gctggtgccc gtggtcagcg
     1501 atgcgcagga caagaccact cgggagctgg cgaccgtgat cgcgcggctc atccgcgacg
     1561 cacgggccgg cacgctcaaa ccgtcggaac tgcaaggctc gacgttcacg gtgtccaact
     1621 tcggcgcgct ggggttggac gagggcgttc cggtgatcaa ctatcccgag gcggccatcc
     1681 tcgggatggg ctcactcaag ccgcgcccgg ccgtcgtcga taacgaagtg gttgtgcggc
     1741 cgacaatgtc actgacgtgt gccttcgacc atcgcatcgt cgatggcgct caggtggcgg
     1801 cattccttcg cgagttgcgc gacctcgtcg aggcgccgga actcgcgctg ctcgatctct
     1861 agtcagcagg ggtcgccggg cggtgtgtag tacggcacgc cctcgaccgt gtagcacggt
     1921 atctcgccgg gaacgtacgg atcggtggcc gccgctatgg ccacgcccgt cgccacgtcc
     1981 ccggcgatgt tggtgcagcc acccactccg acatggcgcc cgtgcgcgcc tgcgcagatg
     2041 tcggcctggg ccaccggcgc gacagccacg ggcaccactg ccgcagccgc ggcggcagtc
     2101 attagcactg ctgccagctt cttcatctca tgctcctctt gctgacccgg cccacctcag
     2161 cgtatgccgc attcctgaag cccgggtcac tttcgccggg cggcagccaa ccgtcttgcg
     2221 aactccggcg aggttattga ggcggcttga ggtcccagtt cggtgtcgac ggcgatccgg
     2281 tgctgttcgg tatcgattac cccgggattg gcggtcgcgc gcatggaggc cttggtggcg
     2341 atgaccacgc cgcggggtgc ggcggccggg ccggcggcga gttcgcgtgc ggccgcgacg
     2401 ggatcgtcgg cgatctgcag cgccagcccg tagcgcaccg cagcctcggc atcgaagcgc
     2461 atgccgaaaa gcagtgaggc gcgggccact tgggggccga cggcgcgctg aagcatccac
     2521 gtggcgccgc cgccgggatg gatgccgagt ttctggaacc gcgggtcgaa aagcgctgcc
     2581 ggaccggcga tccggacatc ggcggccagt gccagattca acccggcgcc gacggcggct
     2641 ccgttgaccg cc
//
LOCUS       DASRJA010000560         4277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3320160,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000560 DASRJA010000000
VERSION     DASRJA010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3320160"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFC01_24730"
     CDS             <1..356
                     /locus_tag="VFC01_24730"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HZN82855.1"
                     /translation="VIGAVNFFDTITGALDEGKLRIGQALADAATIGLLQHRAIHRRG
                     VLTEQLQTALNSRVLIEQAKGLLAERLHVDVAEAFALLRHGARSRNRRLSELAQAIVD
                     GSEQIPPATTTSQRR"
     gene            736..1518
                     /locus_tag="VFC01_24735"
     CDS             736..1518
                     /locus_tag="VFC01_24735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82856.1"
                     /translation="MKRKTLDVLFSGGLLVLAGLLLVAGLVLSSNANFANNYVTDQLS
                     QQNITFKTADTLTDEEKQADCLITNAGQKLTTGKQAECYANNFIGLHLKSTANGQTYA
                     ELGVPQSQLRTQIAQAETANAPNLAELQKQLADVTSQRETLFKGETLRGLLLTSYGFS
                     EFGTKAGQAATVAYLAAGLMLLLAIAGFLHALVTPRSKAFAAPDPAPSRIDDMNTGVN
                     ADGTHTEAARARRATATGHVSNATQHQERRYSDVTDPTKELA"
     gene            complement(1677..2621)
                     /locus_tag="VFC01_24740"
     CDS             complement(1677..2621)
                     /locus_tag="VFC01_24740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82857.1"
                     /translation="MTDQRQSTRDQWAPPAPTLEQADPDWTGPASSSQNSAAKDLTDA
                     ERPAVDGTVYESAAHESAIDESVPADRSDLDDADPEHADLEHADLEHADPEPAVGVAP
                     VPDAVAATDTEADTVDRGDFDPPGGVAQDAARIDGPNGAYGIKGNPADAPAAVEEQEP
                     TEEHGSVAVATDTGQPVDGFDERVEPADGEVPLVPVPGDDAVASDVTGSGELLPGDVP
                     DEPGLALLDGDTTGRFRDRWQELQLRFVDDPHTAADQAGALVDEVVAALRDAVDGQRS
                     ALEEWQSGDGIDTHFGDTERLRVAVRRYRDFLDRLLGL"
     gene            complement(2618..3271)
                     /locus_tag="VFC01_24745"
     CDS             complement(2618..3271)
                     /locus_tag="VFC01_24745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82858.1"
                     /translation="MTTAGIVILIVVGVVVVALVVGGWLITRRRALQKRFGPEYDRVA
                     AEKPSRAAAEQELRGRERRHSELELRPLAPDARARYAAEWEEIQARFVDSPSHVVREC
                     DALVTRLITDIGYPTDGADERLALLSVEYAGTLGRYRDAHEISLRDARGEASTEQLRQ
                     ALVHYRTLFAELLGEQPVAQTDEPIATEPGVSGPVEDGAINNSVPTPVPAGPPRRHS"
     gene            complement(3479..3679)
                     /locus_tag="VFC01_24750"
     CDS             complement(3479..3679)
                     /locus_tag="VFC01_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013806204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZN82859.1"
                     /translation="MTVNARIKSAVRAVKYKAQETRGRAKQTAGTATGNDTLRREGKA
                     EELKSKLHQIAKRIKEAITPNR"
     gene            complement(3818..>4277)
                     /locus_tag="VFC01_24755"
     CDS             complement(3818..>4277)
                     /locus_tag="VFC01_24755"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN82860.1"
                     /translation="GSALAGVLAAIHAKGIVHCDVKPSNLLVRGHDIRVIDFGIARYV
                     GEPCADDGIVHCTRGWAAPEQLRAAPATPAIDVFAWGCLLAHMASGVHPFASQSEQEW
                     ILRLQAAEPDLLGLPPDLDDLIRSTLARNPKDRPNAHELATICQGAPCQY"
BASE COUNT          670 a         1464 c         1384 g          759 t
ORIGIN      
        1 aggtcatcgg cgcggtgaac tttttcgaca ccattacggg cgcgctcgac gagggcaaac
       61 tgcgtatcgg ccaggccctg gccgacgcgg ccaccatcgg gctgctgcag caccgcgcca
      121 tccatcgtcg cggcgtcctc accgaacagc tgcaaaccgc gctgaacagc cgcgtcctga
      181 tcgaacaggc caagggcctc ctggccgaac gcctccatgt ggacgttgcc gaagccttcg
      241 cgctgcttcg ccacggggcc cgcagccgca accggcgcct atctgaactc gcccaggcca
      301 tcgtcgatgg ctccgaacag atcccgccag caacgaccac cagccagcgc cgctgagctg
      361 accgatctgg tcgtcgttcg gctacgcgat gccgccgtgc caaatgggcg tgtggcgcgg
      421 gttcaacgat catgtcgatc gttcactcgg tgctgtccgg ggcaatgacg tgtagggtga
      481 ttttgtcgct cgggtccccg gtggccgtct gcccgccacc gccttcgatg tacccaccag
      541 tcgaggtcgt catgaccgag tagatctcta tgtgtccgac ccgatcggta tctcgcaaac
      601 gacatctgtc ggcaatctgg tggacgacgc gtgcgggcgc gtcgtcgccc ttcgacctgg
      661 cccgcgaacg ctcgtattgc agaacccggt tcgagctgag ccatcgggca ggccccaagc
      721 aacggaaggg atccaatgaa acgcaaaacg ctggacgtcc tattcagcgg gggtttgctg
      781 gttctcgccg gcctactgct cgtcgccgga ctcgtcctat cttcgaacgc gaacttcgcg
      841 aacaactacg tcaccgacca actcagccag cagaacatca ccttcaagac ggccgacacg
      901 ctcaccgacg aagagaagca ggccgactgc ctcatcacca acgccggcca gaagctcacc
      961 accggcaagc aggccgagtg ctacgccaac aacttcatcg gcctgcacct aaagtcgacg
     1021 gccaacggcc agacctacgc tgagctcggc gtcccccaaa gccagctccg cacacagatc
     1081 gcccaggccg agaccgccaa cgcgccgaat cttgccgaac tgcagaagca gctcgcggat
     1141 gtcaccagcc agcgcgaaac tctcttcaag ggggagacgc tacgcggcct gctgctcacc
     1201 tcgtacggct tcagcgagtt tggcaccaag gccggccagg ccgccaccgt cgcctacctc
     1261 gccgccggcc tcatgctgct gctggccatc gccggattcc ttcacgcttt ggtgacaccc
     1321 agaagcaagg ccttcgcggc gcccgacccg gcgccaagcc gcattgacga catgaacact
     1381 ggggtgaatg cagatggcac ccatacggaa gcggcacgag cacgccgagc caccgcgacc
     1441 ggacacgtca gcaatgcgac ccagcaccaa gaacgccgat actcggacgt gaccgatccg
     1501 accaaggagc tcgcatagcc cttcccgtcc ctcacggcga ggagggtctc ccgagcctat
     1561 gcggccccac tgccatgtcg gcgctgtcgc gatcagcgtt ccgcacccgg ctgtagcaat
     1621 ctcctgctcc gcggcttcgg tgccggccct ttgtcaggtg gacggtgttg gggcagtcac
     1681 aggccgagta ggcggtccag gaagtcccgg tagcggcgga ccgcgacccg cagtcgttcg
     1741 gtgtcgccga agtgtgtgtc gatgccatcg ccggactgcc attcctccag tgcggagcgc
     1801 tgcccgtcca ccgcgtcacg cagcgcggcg accacctcat ccaccagcgc gccggcctga
     1861 tccgccgcgg tgtgcgggtc atcaacgaag cgcagttgca actcctgcca ccgatcccgg
     1921 aaccgtccgg tcgtgtcgcc atcgagcagc gcgaggcccg gctcgtccgg cacgtcgccg
     1981 ggcagcagct caccggagcc ggtgacgtcg gacgcgacgg cgtcatcgcc gggtaccggc
     2041 acaagcggca cctcaccatc ggcgggttcg acgcgctcgt cgaagccgtc caccggctgg
     2101 ccggtgtcgg tggccacagc gacgctgccg tgctcctcgg tcggttcctg ttcctccacg
     2161 gcggccggcg cgtcggccgg gttccccttg atgccgtacg ccccgttcgg tccgtcgatc
     2221 cgggcggcgt cctgggcaac gccacccgga gggtcgaagt cgccgcgatc gacggtatcg
     2281 gcctccgtgt ctgttgccgc gacggcgtcc ggtactggcg ccacgccgac ggcgggctcg
     2341 gggtcggcat gttcgaggtc ggcgtgctcg aggtcggcgt gctcggggtc ggcgtcgtca
     2401 agatcggagc ggtcggccgg gacggattcg tcgatggccg actcgtgcgc ggcactctcg
     2461 taaacagtgc cgtccacggc tgggcgttcc gcgtcagtca agtccttcgc cgcactgttc
     2521 tgagaggacg atgccggtcc ggtccaatcc ggatcggcct gttccagggt aggggccggc
     2581 ggcgcccatt gatcacgggt gctttggcgt tggtcggtca tgaatgcctc ctcggcgggc
     2641 cagcgggtac gggcgtcggg accgagttgt tgatggcgcc gtcctcaacc ggcccactca
     2701 cgcccggttc tgtggcgatc ggctcatcgg tctgggcgac gggctgttcg ccgagcagtt
     2761 cggcgaacag cgtgcggtag tgcacaagcg cctggcgcaa ctgctcggtg ctggcctcgc
     2821 cgcgcgcgtc gcgcaacgag atctcgtgcg cgtcgcggta gcgaccgagg gtaccggcgt
     2881 attcgacgga gagcagcgcg agccgctcgt cggcgccgtc ggtcgggtac ccgatgtcgg
     2941 tgatcagtcg ggtgaccaac gcgtcgcact cgcgtaccac gtggcttggc gagtccacga
     3001 agcgagcctg tatctcctcc cactcggccg cgtaccgtgc ccgggcgtcc ggcgccagcg
     3061 gccgcaactc cagctcactg tgcctgcgtt cgcgcccccg cagttcctgt tccgccgcgg
     3121 cgcggctggg cttctcggcg gccacccggt cgtactccgg gccgaatctc ttctgcaggg
     3181 cgcgccgcct ggtaatcagc cagccgccga cgacgagcgc gaccaccacc acgccgacga
     3241 cgatcaggat gacaattcca gcagtggtca tgattgatcc ctccttcgtt ggggtggttc
     3301 cctgacccct tgaattccaa actgtcccgc gtgccgatcc acgctgacgt tcggtcgtcg
     3361 cgcgacgggc atgaggacga agcacgtgat ataggccgcc ccatcgatgc cgccatggcg
     3421 gtcagcaaca cagttgggtg gcggcccgtc gaccagcgga gcggcacgtg ctcgtgacct
     3481 accgattggg cgtgatggct tccttgatcc tctttgcgat ctggtggagt ttgctcttga
     3541 gttcctcggc cttgccttcg cggcgaagtg tgtcgttgcc ggtggctgtg ccggctgtct
     3601 gcttcgccct gcccctggtt tcctgggcct tgtacttgac ggctcgaacc gcgctcttga
     3661 tcctggcgtt cacggtcatg gcgtacggtc tccttatgag gtcgggcatg gcgtgctgct
     3721 gaagagcgtc aacggcgcgt cggcgctgtc gctgtagaga acatggcctc cttgcctttc
     3781 ggtctcgttg tcccacgggc cacggcggta agggcactca gtattggcac ggcgcgccct
     3841 gacagatcgt ggcaagttcg tgtgcgttgg gccggtcctt ggggttgcgg gccagcgtgg
     3901 agcggattag gtcgtcgagg tctgggggta ggccgagcag gtcgggttcc gcagcctgga
     3961 ggcgcaggat ccattcctgt tcgctctggc tggcaaacgg gtgtacgccg ctggccatgt
     4021 gggcgagtag gcaaccccac gcgaagacgt ctatcgccgg agttgctggt gcggcgcgca
     4081 gttgttccgg tgcggcccag ccgcgggtgc agtgcacgat cccgtcatcc gcgcacggct
     4141 cgccgacata tcgggcgatg ccgaagtcga tcacgcgtat gtcatggccg cgtacgagca
     4201 gattcgacgg cttcacgtca cagtggacga ttcccttggc atgtatcgcg gcgagcacgc
     4261 cagccagtgc cgatccg
//
LOCUS       DASRJA010000561         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3976040,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000561 DASRJA010000000
VERSION     DASRJA010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3976040"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFC01_24760"
     CDS             <1..326
                     /locus_tag="VFC01_24760"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212363.1"
                     /note="PlsB; catalyzes the formation of 1-acyl-sn-glycerol
                     3-phosphate by transfering the acyl moiety from acyl-CoA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HZN82861.1"
                     /translation="YMIVADVLSRSEDEPDENELIARALGVGRQYFAQGWLRSNESVS
                     SLLFATAGRVAADQGLLQPGGDQKSRREAFGAELRAVLADLDVLEQFSPTQRVSAARS
                     PGQLR"
     gene            complement(306..1277)
                     /locus_tag="VFC01_24765"
     CDS             complement(306..1277)
                     /locus_tag="VFC01_24765"
                     /inference="COORDINATES: protein motif:HMM:NF014954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZN82862.1"
                     /translation="MGTGQMADSYRLRLDYAHQPDGAPDTVIAKVSSTDEASRQMAIA
                     TGAYQREVLFYRHLSGLTDARCPTCFFADIADDLCGFVLLLEDMGPAKSVDQLTGCSP
                     DQADLALAQAAALHGASWNHPKLAEQSWLPVEHVWSALGGAVPQITDPWLERFGAYLK
                     PDHVAAVHQLGREVTSWLSTLREHRTLWHGDFRLDNLLFDAQDGRTPIAVVDWQSTAA
                     APGIIDVSYFLGNSLTEDDRAIHERDLVTEYHRRLLSYGVEDYTAEQCWREYQAHALY
                     GLVLTIPVSLGVETTERGDAMFGAMASRAAEQIVANDSFAALAELTR"
     gene            complement(1408..2454)
                     /locus_tag="VFC01_24770"
     CDS             complement(1408..2454)
                     /locus_tag="VFC01_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03857 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HZN82863.1"
                     /translation="MPEIGFYTLAGQADQPRDLIREVTHAETLGIGECFISERYCTKE
                     AATISGAVGAVTESIGITTAATNHNTRHPMVTAAFATTMHRLTRGRFTLGIGRGIPPL
                     QRAFGMSEITTAQMEDFAHVMRRLFRGETVVGHDGPIGRYPALRLDPDFDEDVKLGIV
                     AFGANSLALGGRAFDKVILHTFFTDETLARCVRTVKDAAEAAGRNPQRVQVWSCLATI
                     GDHMPEELRLRKTVGRLATYLQGYGDLLVSTNNWDPLALKRFRDDPVVASFHPPGGAL
                     RVIDSPSTTIDQLEHIATLLPDEWLAPAATGTPQQCAATVKTQLSLGADGVILHGASP
                     SELAPILPAYRAIR"
     gene            complement(2463..3254)
                     /locus_tag="VFC01_24775"
     CDS             complement(2463..3254)
                     /locus_tag="VFC01_24775"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005201800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZN82864.1"
                     /translation="MTCRFKDRTVLLTGAARGMGESHARAFAAEGARVVIADVLDDEG
                     HALADELGDAALYVHLDVSDIEGWRMALERTENLFGPLHVLINNAGIGGLPATLVDTD
                     IDQWRRVLAVNLNGTFYGIKVAAPSIIRAGGGAIVNVSSFAGLMGSPMLGGYAASKFG
                     VRGLTKVAAMELGPHGVRVNSIHPGYIRTALLEGIPDAAVRGRLAIERVATPTDVTPV
                     VLFAASDEAAYCTGAEFAVDGGWSAGEPSPIFVAPGAAMPEGVAL"
BASE COUNT          563 a         1052 c         1128 g          593 t
ORIGIN      
        1 catacatgat cgtggccgac gtgctgagtc gatcggagga cgaacccgat gagaacgagc
       61 tgattgcaag ggcattgggg gtaggccgcc agtatttcgc tcagggatgg ctgcgcagca
      121 acgagtcggt gtcgtcgcta ctgttcgcca cagcaggtcg cgtggccgcc gaccagggct
      181 tgttgcagcc gggcggcgac cagaagagcc gacgcgaagc gttcggtgcc gaattgcgcg
      241 ccgtgctggc cgatctcgat gtgctcgaac aattctcacc cacccagcgg gtctccgctg
      301 cacgatcacc gggtcagctc cgctagcgcg gcgaagctgt cgttggccac gatttgttca
      361 gcagcacgac tggccatcgc gccgaacatg gcgtcgccgc gttcggtcgt ttccacgccc
      421 agcgagaccg ggatggtcag cacgaggcca tagagggcgt gcgcctggta ctcgcgccag
      481 cactgttccg cggtgtagtc ctcgactcca taggacagca ggcgcctgtg gtactcggtg
      541 accaggtcgc gttcgtggat ggcccggtcg tcctcggtca aggagttgcc aaggaagtag
      601 gacacgtcga tgatcccagg cgcggcggcg gtgctttgcc agtcgaccac ggcgatcggc
      661 gtccggccgt cctgcgcatc gaagagtagg ttgtccagac ggaagtcgcc gtgccatagc
      721 gtccggtgct cgcgaagagt cgacagccac gatgtcactt cgcgcccaag ctggtgaacg
      781 gccgcaacgt ggtcaggctt caagtaagcg ccgaatcgct ccagccacgg gtccgtgatc
      841 tgcggaaccg ccccgccgag ggcggaccag acgtgttcga caggcagcca gctctgctcg
      901 gcgagtttcg ggtgattcca cgaggccccg tgcagcgcag cggcctgggc cagggcgagg
      961 tcggcctggt cgggactgca acctgtcaac tggtccacac tcttggcggg ccccatgtct
     1021 tccagtagca gcacgaaccc gcacaggtcg tcggcgatat cggcgaaaaa gcatgtcgga
     1081 caacgcgcgt cggtgagacc gctcaggtgc cggtaaaaca gcacttcgcg ctgatacgcg
     1141 cctgtggcga tcgccatctg cctgctcgcc tcgtcggtgc tggacacctt ggcgatgacg
     1201 gtgtcgggcg cgccgtcggg ctggtgagcg tagtccagcc gtagccggta gctgtcggcc
     1261 atctgtcccg tgcccaccgg tacggcatgg acgcgttcga tctcgacccc tgccagccgc
     1321 gcgtcgcagc cagcatgccg ttcaggacat cgggggtgaa ttcggcgatg gtgtagtcgg
     1381 aaaccgtcat caggatcggc ctttcgttca gcgaatcgct cggtaggcgg gcaggatcgg
     1441 ggccagttcg ctcggcgagg cgccgtgcag gatcacaccg tcggcgccga gggataactg
     1501 agtcttcacg gtggcagcgc actgctgcgg cgtgccggtt gccgcgggcg ccagccactc
     1561 gtcgggcagc aacgtggcga tgtgttcgag ctggtcgatc gtcgtcgacg ggctgtcgat
     1621 aacccttagt gcaccgccgg gtgggtggaa acttgctacc accggatcgt cgcggaaccg
     1681 cttgagcgcc aatggatccc agttgtttgt tgataccaga aggtcgccgt atccctgcag
     1741 gtacgtggcg agccggccca cggtcttgcg cagacgcagt tcctcgggca tgtgatcgcc
     1801 gatggtcgcc agacacgacc acacctgtac gcgttgggga tttcgaccgg ctgcctcagc
     1861 tgcgtccttg acggtgcgca cacagcgcgc gagcgtctcg tcggtgaaga acgtgtgcag
     1921 aatgaccttg tcgaacgcac gcccgcccaa cgcaagcgag tttgcgccga acgcgacgat
     1981 gccgagtttg acgtcctcgt cgaagtccgg gtccaagcgc agcgcggggt agcggccgat
     2041 cgggccgtca tgacccacga cagtttcgcc gcgaaacaac cggcgcatca cgtgggcgaa
     2101 gtcttccatt tgcgccgttg tgatctcgga catgccgaaa gcccgctgca gcggcggtat
     2161 cccacggccg atgcccaggg tgaagcgtcc gcgggtgagc cggtgcatcg tggtggcgaa
     2221 cgcggcggtc accatcgggt ggcgggtgtt gtggttcgtg gcggcggtgg tgatcccaat
     2281 ggattcagtg accgcgccga ccgccccgga tatcgtggcg gcttctttgg tgcaatagcg
     2341 ttcggagatg aaacactcac cgatgcccag tgtttcggca tgggtgacct cgcggatgag
     2401 gtcgcgtggt tggtctgcct ggccggccag cgtgtagaag ccgatctcgg gcattacggg
     2461 cttcacagcg cgacgccctc gggcattgcg gcccctgggg cgacgaagat cgggctgggc
     2521 tcaccggccg accatccgcc atcgaccgcg aactcggcgc cggtgcaata ggcggcttca
     2581 tcgctggctg cgaacagcac caccggggtc acatcggtcg gggtggcgac acgctcgatg
     2641 gccagccggc cgcgcaccgc ggcatccggg ataccctcca gcaacgcggt gcgaatgtag
     2701 ccggggtgga tcgagttgac ccgaactccg tgcggcccca actccatcgc ggccactttg
     2761 gtcagcccgc gcacgccgaa cttgctcgcc gcgtatcccc cgagcatcgg cgagcccatc
     2821 agcccggcga acgacgagac gttgacgatg gcaccgccgc cggcccggat gatcgacggg
     2881 gccgcgactt tgatcccgta gaaggtgcca ttgaggttga ctgccaagac tcggcgccat
     2941 tggtcgatgt cggtgtcgac cagtgtggcc ggcagtccac cgataccggc attgttgatc
     3001 agaacgtgca gtggcccgaa taggttctcg gtgcgctcga gtgccatccg ccagccctcg
     3061 atgtcgctga cgtcgaggtg gacgtagagt gccgcgtcgc cgagttcgtc agccagcgcg
     3121 tggccttcgt cgtcgaggac atcggcaatg acgactctgg cgccctcggc ggcgaacgca
     3181 cgggcatgcg attcccccat tccgcgggcc gctccggtca atagcacggt gcggtcctta
     3241 aaccggcatg tcatggaatc agtaccgccc ttccgtcatt ggtccattcc gaagatcgtg
     3301 gcggtccaaa tgcgcaccaa cgaggcgtgc aaggcg
//
LOCUS       DASRJA010000562         8632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_718617,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000562 DASRJA010000000
VERSION     DASRJA010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718617"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..451)
                     /locus_tag="VFC01_24780"
     CDS             complement(20..451)
                     /locus_tag="VFC01_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZN82865.1"
                     /translation="MRIADVLRNKGASVATITPETSVSGLLTELTVHNIGAMVVMSQD
                     GLVGIVSERDVVRKLHELGADILRRPVSEIMTTVVATCTPDDSVNSLSVLMTEKRVRH
                     VPVVVNGRLAGIVSIGDVVKTRMEELETQQQQLEAYITQGR"
     gene            complement(534..1121)
                     /locus_tag="VFC01_24785"
     CDS             complement(534..1121)
                     /locus_tag="VFC01_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN82866.1"
                     /translation="MSSLRERKRVRTRKALIDAAAELFEQRGYDGTTIADIAAAADVS
                     TRTFFSYFASKEDVLFPDADARVKAALTAIDERRPGERPTAILLRALGELGDAGADLV
                     GPMAALRLRFVRSVPSVRGRGLQRQLDAQTEIAARLQAAFPDELDEVEAAALVGAFVG
                     AIAGALEVLLRDEDDPDVLRERLQSATAIALRPWT"
     gene            1199..2374
                     /locus_tag="VFC01_24790"
     CDS             1199..2374
                     /locus_tag="VFC01_24790"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZN82867.1"
                     /translation="MRKALISGAGIAGSTTAYWLAKAGFEVTVTEQAQDLRSSGSPVD
                     VRGAAVEVAERIGVMARIRTADTRVRNMVFVNSRGRVVSRVNMRTTWAESGDVELSRG
                     ELAAILRDAVPDDVEFQFGNAVTALAQDAAGVTAEFVSGPARRFDVVIGADGAHSGVR
                     ALAFGPESDYLKHLGVYVATLPLAGETGTDLVLYNTPGRAVAIHPAGGHPGAAFMFRA
                     PQIPQFDHRDVEQHKRLLCDAYAGAGWRVPELLDRVRAADDLYFDSVSRIQVPTWSRG
                     RIGLVGDAASCVSLFGDGSSLAMIGAFTLADSLDGDISAGLRSYEARHRPQRAAKENS
                     VAYATRLLIPATSAGIALRNAAMHLMPLASAAARLGIPRGKWHSRSGLPEYSFGETR"
     gene            2383..3405
                     /locus_tag="VFC01_24795"
     CDS             2383..3405
                     /locus_tag="VFC01_24795"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN82868.1"
                     /translation="MTSANSVPAVKLGDELTVSAIGFGAMALTPVYGEVDDTESLATL
                     HRTVDLGVTFIDTANIYGNGNNEKLIAKLLADRRDEVTLATKFGIQGNPADRAAGRIG
                     VRGDAAYVRQCIDESLQRLQTDVVDLYYMHRRDVNVPIEETVGAMAELVAAGKVRHLG
                     LSEVTADELQAAVAVHPIAAVQSEWSIWSRDVERNVVPAAAELGVGFVPYSPLGRGFL
                     TGTVRSAADLSSPNDFRKTMPRFSGGALDANLAVVEVVKSVAAQQGVTPAQVALAWLR
                     HRADALGVASVPIPGTRRAARVEENLGSLSVTLTPEQLDTLDAAGAAVSGNRFADMTW
                     VSAGRE"
     gene            complement(3406..4125)
                     /locus_tag="VFC01_24800"
     CDS             complement(3406..4125)
                     /locus_tag="VFC01_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82869.1"
                     /translation="MAKIHAIGRPRNKTATAPTTSPRADIVNAATKLFSEKGYAQTTM
                     SDIARASGLQQSSLYYWFRNKEQLLQETLLVNRAPLKFIAEVGAGSGSPAVKLYRLLR
                     FDTMQLALSPIDFNEIQRIAHDQRTEFHQFWTDYERLKNWVVDLIGAAISEGKFIDCD
                     REETAGLLLNFDEGAQKRTRLHSDEGDRADEAIRVGEQVAIIAVRGLLKRPSEIARIS
                     AQAAQFDDAAIALRVARPQPD"
     gene            complement(4118..4861)
                     /locus_tag="VFC01_24805"
     CDS             complement(4118..4861)
                     /locus_tag="VFC01_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZN82870.1"
                     /translation="MTHLWEELTSAQLADRLAEDPDCVALIPVGATEQHGPHLPVGTD
                     TIIATALADAAAGEVAVVLPPLAFGASFFHGTRLAGTVAFSGEETAATAGLVAEACVD
                     SGFRRVLFVNGHVGNAAALWISCDRFRREFPELRIGVVQWWDLTPDIARRATEDAADW
                     HANAAETSIMLVLRPELVDTDRMREADDPDRTEGTVFRYAVQHVSTNGVTGYPSCASK
                     SIGLELWQDVVAAARDLVERAHSEKPPLG"
     gene            complement(4864..6234)
                     /gene="glnT"
                     /locus_tag="VFC01_24810"
     CDS             complement(4864..6234)
                     /gene="glnT"
                     /locus_tag="VFC01_24810"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HZN82871.1"
                     /translation="MTTTLNGQTAIASTARDVADALAAKGVKYAAISFVDMHGKPKSK
                     MVPLGHLTQAALGSEMFTGAALDGVPQDVNDDEVAPHPDLDHPIVMPFNPEIAWFPGD
                     LWIGETPFEACSRQILKRVTADAATLGYTFNLGIETEFFLLTDSEQPGPASPDDNLDK
                     PCYDLRTMLHNYPVVDEIVSAMNELGWDVYSFDHEDGNGQFETDFAYTDVVGMSDRLV
                     FFRMMVGEIARKHGLFASWMPKPFADRTGSGAHYNMSLADAAGTNLFAADDDPRECGL
                     STLGYQFIAGVLRHVDAICAVIAPTVNSYKRLVKQGSMSGLTWAPVFACYGDNNRTNM
                     LRIPRGGGRVECRAADSANNPYLGGALMLAAGLEGIREGLDPGEPNRDNLYRLTEPEL
                     VTRGIGWLPRSLGEAIDALEADPLVRQVFGDAMFESFVAEKRAEWDGYTAHVSSWETD
                     RYLRFW"
     gene            complement(6243..7406)
                     /locus_tag="VFC01_24815"
     CDS             complement(6243..7406)
                     /locus_tag="VFC01_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZN82872.1"
                     /translation="MTFDYTRLTATCAVESPDKAITLPPEAYGSDDLYAAEVDRIFKR
                     GWIPLCRVEQVSTPGSYYSIDILGTPLVVTRDRHSEIRVLSRNCTHRWMEVCSGAGEA
                     NVLQCPYHLWSFGLDGHLAGAPEMKKSPGFDRNDYPLKKFRHEVWKGFVMVNFDGQAE
                     SLASQYAALDPIVDAYDLENYQTVEQTDWGVCDWDWKIMVDNFMECYHHMGPHRGSLE
                     DRFPATMSFTGEVGDYFTTMWSQQRPEYPADPPFLSPGADTLTAEHKDRSLIFIAYPL
                     LQIAMGPAYMYWLKTLPLGAGKIELQLDICMSPASLAAPNVEERRSELVKLICDIHRE
                     DLDVCAAVQRAVRAGVTSTGRLSHLERPLWEFYRYLGRELGLINPTTTLASAG"
     gene            7593..8453
                     /locus_tag="VFC01_24820"
     CDS             7593..8453
                     /locus_tag="VFC01_24820"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HZN82873.1"
                     /translation="MIEVTLLGTGSPIPDARRAGPSTLVRAGGQTFLVDCGRGVQQRM
                     TAAGTGANVLTALLLTHLHSDHIADLGDVLITRWVTTFDPNPVPLPIIGPPGTAEVVD
                     ASLKAFGFDIGYRIAHHADLTTPPPVEVHEYTDGDVWDRDGVTIRVAPTDHRPVAPTI
                     GFRIEHGDASVVLAGDTVPCETLDALAAGAGGLVHTVIRKDLIDKMPMQRIRDICDYH
                     SSVEQAAATAARAGVGILILTHYVPGIEPGQEDDWRALAATAFDRQIEIGDDLHRVEV
                     HPGVCARTVT"
BASE COUNT         1436 a         2812 c         2874 g         1510 t
ORIGIN      
        1 cgggcgtcta cggacgccat taacgaccct gagtgatgta ggcctcgagc tgctgctgct
       61 gggtttcgag ttcttccatc cgcgtcttga ccacgtcgcc gatgctgacg atcccggcca
      121 gccggccgtt cacgaccaca gggacatgcc gaacccgctt ctcggtcatc aacacgctga
      181 ggctgttgac ggaatcgtcc ggggtgcagg tcgccaccac cgtcgtcatg atctctgaca
      241 ccggccgccg gagaatgtcg gcgcccaact cgtgcagttt gcgcacgaca tcgcgctccg
      301 acacgatgcc gaccaaccca tcctgcgaca tcaccaccat ggcgccgatg ttgtgcacgg
      361 tgagctcggt gagcaggccg gagacggacg tctccggggt gatggtcgcc accgacgcac
      421 ccttgttccg caatacgtcc gcgattcgca tcgatgcctc cgctctcgtg acctaattca
      481 caggctaggg ccgacgcgcc cgtcacgaaa gagattccga ggttgcaacc gggtcacgtc
      541 cacggccgca acgcgatggc cgttgcgctc tgcagacgct ctcgaagcac gtcgggatcg
      601 tcttcgtcgc ggagcaaaac ttccagcgcg ccggcgatgg cgccgacgaa cgccccgacc
      661 aacgccgcgg cctcgacctc gtccaactcg tcggggaacg cggcctgcag ccgggccgcg
      721 atctcggtct gcgcgtcgag ctggcgctga agaccccgac cccgcacgga cggaaccgat
      781 ctcacgaacc gcagccgcag cgccgccatc gggccgacca ggtcagcgcc ggcgtcaccg
      841 agttcaccca acgcgcgcaa cagaattgcg gtcggacgtt cgccgggtcg gcgctcatcg
      901 atcgcggtca gcgccgcctt cacccgcgca tcggcgtcgg gaaacaacac gtcctcttta
      961 ctcgcgaagt acgagaagaa ggtgcgggtg ctgacgtccg cggcggccgc gatgtcggcg
     1021 atcgtggtgc cgtcgtaacc gcgttgttcg aacagctccg cggcagcgtc gatcagcgcc
     1081 ttccgggtgc ggacgcgttt gcgctcgcgc agtgacgaca tgcccggagc ctaccgcgaa
     1141 actgagagtt ttctgcattc attgcaattt tgcacttact gtaattagcg tggtcgatat
     1201 gcgaaaggca ttgatctccg gcgcgggcat cgccggctcg acaacggcat attggctggc
     1261 caaagctggt tttgaagtga cggtcacaga acaggcgcag gacctgcgat cgagcggcag
     1321 cccggtcgac gtccgaggcg ccgccgtcga ggtggccgag cggataggag tcatggcgcg
     1381 gatccgcacc gccgacacac gcgtgcgcaa catggtcttc gtcaactcgc gcggccgcgt
     1441 ggtcagccgc gtcaacatgc gcaccacctg ggccgaatcc ggcgacgtcg agctatcccg
     1501 cggtgagctc gcggcgatcc tgcgcgacgc ggtgcccgac gacgtggagt ttcagttcgg
     1561 caacgcggtg acggcgctcg cccaggacgc ggccggagtc acggcggagt tcgtatcggg
     1621 gcccgcgcgt cgattcgacg tggtgattgg tgccgacggg gcgcactcgg gtgtccgagc
     1681 gttggccttc ggccccgagt ccgactacct caaacacctc ggcgtgtacg tcgccacgct
     1741 gccgctagcc ggggaaaccg ggacagacct cgtcctgtac aacacgcccg gtcgagccgt
     1801 ggcgatccac cctgcaggtg gccacccggg cgccgcgttc atgttccgcg ccccgcagat
     1861 cccacagttc gatcaccggg acgtcgagca gcacaagcgt ctgctgtgtg atgcgtatgc
     1921 cggagcaggc tggcgagtgc ccgaactgct ggaccgggtc cgcgccgccg atgacctgta
     1981 cttcgactcg gtcagccgga ttcaggtgcc gacgtggtct cgaggtcgga tcggcctggt
     2041 cggtgacgcc gcatcgtgtg tctcgttgtt cggcgacggc tccagtctgg cgatgatcgg
     2101 ggcgttcacc ctggccgact cactggacgg cgacatatcg gcgggtctgc gcagctatga
     2161 ggcgcggcac cgaccccagc gcgcggccaa ggagaactcc gtggcatacg cgacgcggct
     2221 gttgattccc gccacgtcag cgggcatcgc cttacgcaac gccgccatgc acctgatgcc
     2281 cctggccagc gcggcggcac ggctgggcat cccccgtggg aaatggcatt cccgtagcgg
     2341 cctgccggaa tacagtttcg gcgagactcg ttaggtcaag ccatgacaag tgccaactct
     2401 gtgcccgcgg tgaagctggg tgacgaatta actgtcagtg caatcgggtt cggcgccatg
     2461 gcgttgacac cggtatacgg cgaggtcgac gacaccgagt cgctggccac cctgcaccgc
     2521 acggtggacc tcggcgtgac attcatcgac accgccaaca tctacggcaa cggcaacaac
     2581 gagaagctca tcgccaagct gctcgccgac cgccgcgacg aggtcacgct cgccaccaag
     2641 ttcggcatcc aggggaaccc cgctgaccgc gccgcaggcc gcatcggcgt ccgcggcgac
     2701 gcggcgtacg tgcgccagtg catcgacgag agcctgcagc ggctacagac cgacgtcgtc
     2761 gatctgtatt acatgcaccg ccgcgacgtg aacgtgccga tcgaggagac tgtcggcgcc
     2821 atggccgaat tggtggccgc gggcaaggtg cgtcatctcg ggctgtcgga ggtgactgcc
     2881 gacgagctgc aggccgccgt cgccgtgcat ccgatcgccg cggtgcagag cgagtggtcg
     2941 atctggagcc gcgacgtcga acgcaacgtc gtgcccgcgg ccgccgagct cggtgtcggc
     3001 ttcgtgccgt attcgccgct ggggcggggg tttttgaccg gaacggtccg ctcggccgcc
     3061 gacctgtcgt cgccgaacga cttccgcaag accatgcccc gattcagcgg gggcgcactg
     3121 gacgccaacc tcgccgtggt cgaggtcgtg aagtcggtgg cggcgcagca gggtgtgacg
     3181 cccgcgcagg tggcgctggc gtggctgcgt caccgtgccg acgcgctcgg cgtcgcgtcg
     3241 gtgccgatcc ccggcacccg gcgggcagcg cgtgtcgagg agaacctcgg ctcgctgtcg
     3301 gtgacgctga caccggagca gctcgacacg ctggacgccg cgggcgcggc ggtgtcgggc
     3361 aaccggttcg cggatatgac gtgggtgtcg gccgggcgcg agtagtcagt ccggctgcgg
     3421 ccgggcgacc cttagtgcga tcgccgcgtc gtcgaattgg gcggcctggg cgctgatcct
     3481 ggcgatctcg ctgggacgct tcagcagtcc gcgcacggcg atgatcgcga cctgctcgcc
     3541 gacccggatg gcctcgtcgg cgcggtcgcc ctcatcgctg tgcaagcggg tgcgcttctg
     3601 cgcgccctcg tcgaagttca gcagcaggcc cgcggtttcc tcgcggtcgc agtcgatgaa
     3661 cttgccctcg ctgatcgccg ccccgatcag atcgacgacc caattcttca accgctcgta
     3721 atccgtccag aactggtgga attcggtgcg ctgatcgtgg gcgatgcgct ggatctcgtt
     3781 gaagtcgatc ggggagagtg ccagctgcat ggtgtcgaac cgcagcagcc ggtacagctt
     3841 gaccgcaggc gagccggagc ctgccccgac ctccgcgatg aacttcagcg gcgcccggtt
     3901 gaccagcaga gtctcctgca gaagctgttc cttgttgcgg aaccagtagt acagcgacga
     3961 ctgctgaaga cccgacgcgc gggcgatgtc gctcatcgtc gtttgggcgt aacccttttc
     4021 ggagaacaac ttggtcgccg cgttgacaat gtcggcgcgg ggtgacgtcg tcggcgcggt
     4081 ggcggtcttg ttgcggggtc gtccgatggc gtgaatctta gccaagcggc ggcttctcac
     4141 tgtgcgcgcg ctcaaccagg tcacgcgccg cggcgaccac gtcctgccac agctcgagcc
     4201 ctatcgactt cgacgcacac gacgggtagc cggtgacccc gttggtcgag acgtgctgta
     4261 ccgcgtagcg gaaaacggtg ccctcggtgc ggtcggggtc gtcggcctcg cgcatcctgt
     4321 ccgtgtcgac cagttcgggc cgcagcacca gcatgatcga cgtctcggcc gcgttcgcgt
     4381 gccagtcggc ggcgtcctcg gtcgcccgcc gcgcgatatc aggcgtcaga tcccaccact
     4441 gtacgacacc aattcgcagc tcgggaaact ctctccggaa gcggtcgcag gagatccaca
     4501 gcgcggccgc attgccgacg tgaccgttca cgaagagcac ccggcgaaag ccagagtcca
     4561 cgcaagcttc ggcgaccagg ccagccgtcg ccgccgtctc ctcgccggag aacgcgaccg
     4621 tgcctgccag ccgtgtgccg tgaaagaagc tggcgccgaa cgcaagtggt ggcaacacga
     4681 cggccacctc ccccgcggcc gcatcggcga gcgccgtcgc gatgatggtg tcggtaccca
     4741 ccggaaggtg cgggccgtgt tgctcggtcg cgccgacggg tatcagcgcc acgcagtcag
     4801 ggtcttcggc aaggcggtcg gccagttgcg ccgacgtcag ctcttcccat agatgggtca
     4861 tggtcaccag aacctcaggt aacggtcggt ctcccacgag gacacgtgcg cggtgtaccc
     4921 gtcccactcg gcgcgcttct cggcgacgaa cgactcgaac atcgcgtcac cgaacacctg
     4981 acgaaccaac gggtccgctt ccagtgcgtc gatcgcttcg cccagtgacc ttggcagcca
     5041 accgattcca cgtgtcacca gctcgggttc agtgagccgg tagaggttgt cccggttcgg
     5101 ctcgcccgga tcgagtccct cgcggatccc ttccagcccg gccgccagca tgagggcgcc
     5161 gccgaggtag gggttgttgg cgctatcggc ggcgcggcat tcgacgcgac caccgcccct
     5221 cgggatgcgc aacatgttcg tccggttgtt gtcgccgtaa caggcgaaca ctggggccca
     5281 agtcaggccg gacatgctgc cctgcttgac cagccgctta taactgttga ccgtcggcgc
     5341 gatgaccgcg cagatggcgt ccacgtgacg cagcaccccg gcgatgaact ggtagccgag
     5401 ggtggaaagc ccgcactcac gcggatcatc gtcggccgca aagaggttcg tccctgcagc
     5461 atcggccagc gacatgttgt agtgagcgcc gctgcccgtg cgatcggcga acggtttggg
     5521 catccacgat gcgaacaggc cgtgcttgcg ggcgatctcg ccgaccatca tccggaagaa
     5581 caccagccgg tccgacatgc ccaccacatc ggtgtaggcg aagtcggtct cgaactgtcc
     5641 gttgccatcc tcgtggtcga acgagtagac gtcccagccg agttcgttca tcgcggacac
     5701 gatctcgtcg accaccgggt agttgtgcag catcgtgcgc aggtcgtagc acggcttgtc
     5761 gaggttgtcg tcggggctcg cgggacccgg ttgctcggaa tcagtgagca ggaagaactc
     5821 ggtttcgatg ccgaggttga aggtgtaccc cagtgtggcc gcgtcggccg tcacgcgttt
     5881 gaggatctgc ctgctgcatg cctcgaacgg ggtttcaccg atccacagat cgcctgggaa
     5941 ccaggcgatc tcgggattga agggcatgac gatcgggtgg tcgaggtcgg gatgcggcgc
     6001 gacctcgtcg tcgttgacat cctgcggcac gccgtcgagc gcagcgccgg tgaacatctc
     6061 cgacccgagc gccgcctggg tcagatggcc gagcggcacc atcttgctct tgggcttgcc
     6121 gtgcatgtcg acgaagctga tggcggcgta cttgacgccc ttggccgcca gtgcgtcggc
     6181 gacatcgcgg gcggtcgatg cgatggcggt ctgtccgttg agggttgtcg tcatctgttg
     6241 tgtcatcctg ccgatgcgag agtggttgtg gggttgatga gtccgagctc gcggccgagg
     6301 tagcggtaga actcccacag tggtcgctcc aggtgcgaaa gccggccggt gctggtcacg
     6361 ccggcccgga ccgcgcgctg cactgcggcg cagacgtcga gatcctcgcg gtggatatcg
     6421 cagatcagct tgaccagctc actgcggcgt tcctcgacat tgggcgcggc cagcgaggcc
     6481 ggggacatgc agatgtcgag ctgaagttcg atcttgccgg ccccgagcgg aagtgtcttg
     6541 agccagtaca tatatgccgg gcccatcgcg atctgcagca gcgggtacgc gatgaagatc
     6601 agcgatctgt ctttgtgctc cgccgtcagt gtgtcggcgc cgggcgacag gaacggcgga
     6661 tccgcggggt attcgggcct ctgctgcgac cacatcgtgg tgaagtagtc gccgacctca
     6721 ccggtgaagc tcatagtggc ggggaatcgg tcctccagcg acccacggtg cggacccata
     6781 tggtggtagc actccatgaa gttgtccacc atgatcttcc agtcccagtc gcacacaccc
     6841 cagtcggtct gctcgacggt ctggtagttc tccaggtcgt aggcgtcaac gatcgggtcg
     6901 agcgccgcgt actggctcgc caaagactcc gcctgcccgt cgaagttcac catgacgaag
     6961 cccttccaca cttcgtggcg gaacttcttg agcggatagt catttcggtc gaatccgggc
     7021 gacttcttca tttccggcgc tccggcgagg tgcccgtcca ggccgaacga ccacaggtgg
     7081 tacggacact gcagcacgtt ggcctcgccg gcgccgctac acacctccat ccagcgatgt
     7141 gtgcagttgc gcgacagcac ccggatctcc gagtggcggt cgcgcgtgac caccagcggg
     7201 gtgccgagga tgtcgatgct gtagtagctg cccggcgtgg acacctgctc gacgcggcag
     7261 agcggaatcc acccgcgctt gaagatccga tcgacctcgg cggcgtacaa gtcatcgctg
     7321 ccgtacgcct cgggcggcag cgtgatcgcc ttgtcgggtg actcgacggc acaggttgcc
     7381 gtcagtcggg tgtagtcgaa tgtcatggca ccgacgctat gggcgctctg ttgcgggtcc
     7441 gtttcgtgac cgtaaatgac ctattgaaat tcggatggcg attgatcggc gccattgcgt
     7501 cagttacatg cgtctttcga ccgacgtctc aatacagtat tgaaacgaag gtttcacttt
     7561 ctgacgtgca cgaaaacggt aggaatgaca ccatgatcga ggtcaccctg cttggcactg
     7621 gcagtcccat tcccgacgcc cgacgagcgg gaccgtcgac gctcgtacgt gcgggcggac
     7681 agacgtttct ggtcgactgc ggacgcggtg tgcagcaacg catgacggcg gccggcaccg
     7741 gcgccaacgt attgacagcg ctactgctga cgcatctgca cagcgatcac atcgccgatc
     7801 tgggcgacgt gctgatcacg cggtgggtga cgacgttcga cccgaaccct gtgccgctgc
     7861 caatcatcgg cccgccgggc acggccgagg tggtcgacgc ctcgctgaag gccttcggtt
     7921 tcgacatcgg ttaccggatc gcgcatcacg ccgatctgac tacgccgcca ccggtggagg
     7981 tgcacgagta caccgacggc gacgtgtggg accgcgacgg agtgacgatc cgggtggcgc
     8041 cgacggacca ccggcccgtc gcaccgacga ttggattccg catcgagcac ggcgacgcct
     8101 cggtggtgtt ggccggtgac accgtgccgt gcgagaccct cgacgcgctc gcggcgggtg
     8161 ctggcgggtt ggtgcacacc gtgattcgca aggatctgat cgacaagatg ccgatgcagc
     8221 gcatccgcga catctgcgat taccactcgt cggtcgaaca ggccgcggcc accgcggcac
     8281 gcgccggcgt cggcattctg atcctcactc actacgtgcc gggcatcgaa ccgggtcagg
     8341 aggacgattg gcgcgcgctg gcggccactg cgttcgaccg gcagatcgag atcggcgacg
     8401 acctgcatcg cgtcgaggtt caccccgggg tgtgtgcgag aacggtcacg taatccggta
     8461 gccccgattg tgcgcatgcg ctcacactga accgcagcaa ggggaggtgt gcggtgggga
     8521 cgaacggcag gggtggcgac gcgctcgcgg atttggctgc cgccgttgga gaagcggatc
     8581 gcggaggcag cctttacgag accgcgaatg ccctcgcacg ggtggttgtc ga
//
LOCUS       DASRJA010000563         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_488781,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000563 DASRJA010000000
VERSION     DASRJA010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488781"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="VFC01_24825"
     CDS             <1..1415
                     /locus_tag="VFC01_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZN82874.1"
                     /translation="GDPDRAALIIDGRVISYGELATAVERCAARLSDSTVAGARVAVV
                     DVGSLASIATMFGAARIGSAAALMNPALTPPELLGLVKTAGCDPTGVAGAEYVDRLLD
                     AGLVAVWTATDLIDDDGAPPAPPPADDVDDREALVLFTSGTTGLPKPIGVTHRRLSAR
                     IYGVTTPFRADAPPVVSMMSVPFFHVGGAIGVLGNLYSGNTYLVQNRFDAGEWLRLVQ
                     AHRVTSTFMVPTMLRRILDHPNFGNTDLSSLTAIAYGAAAAPIALVRRAMDAMPHVGF
                     ANVFGQTETLGAYTTLLPADHRDPNRAGSVGRPLPGVEVRVVDPDTGIDVDVGTVGEL
                     WVNTTQNVTGEDWLHTGDLAREDADGYIYPVGRLKDTINRGGEKFGPIEIEDAIRTHP
                     AVRDVAVAGIADEEMGQRVGAAVVARYPLTLEELRSHCRELIAYFKLPERLKMVERIP
                     YTETGKVNRRQLAALIADGA"
     gene            1431..2135
                     /locus_tag="VFC01_24830"
     CDS             1431..2135
                     /locus_tag="VFC01_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82875.1"
                     /translation="MLFLHETHKVVGAREDEFEAAYREGWMPTLAQGEDARLLWYTNH
                     AHGSGVSYNVVTITGIADGAAWERLALRVQSGDLQPWMRELDNLRHEVTGKILLPVEW
                     SPLQEVDLATVPTDGAAHPLSLFMEDTGWPYAPLDDYIRSWDEIYYRPLSTTSQILDI
                     QACFQVAHGSYRRREAMLWQKISPANDYATLVHLLTSDISPEHRGPGTYMFEALKYRD
                     QWESRLLRTSAWSPLY"
     gene            complement(2159..>2421)
                     /locus_tag="VFC01_24835"
     CDS             complement(2159..>2421)
                     /locus_tag="VFC01_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005568970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82876.1"
                     /translation="DYLSAYVLEWTLHHLDLIAHLPGVAEPPADGLARSREMLETIVG
                     AAFPKLLPDKDALLIGTGRRTPTDTEKAQLGDLAAKLPVVLG"
BASE COUNT          432 a          797 c          789 g          403 t
ORIGIN      
        1 caggggatcc cgaccgcgcc gcgctcatca tcgatgggcg cgtcatcagc tacggcgaat
       61 tggcgacggc cgtcgaacgc tgcgccgcgc gcctgtcgga cagcacagtg gccggcgcac
      121 gcgtcgccgt cgtcgacgtt ggcagcctgg cgtccatcgc gaccatgttc ggtgccgccc
      181 ggatcggctc ggccgcggcc ctgatgaatc ccgcgttgac accaccggag ttgctggggc
      241 tggtgaagac cgccgggtgt gacccaacgg gcgtggctgg agccgaatac gtcgaccgcc
      301 tccttgacgc tggtctggtc gcggtgtgga cggccaccga cctgatcgat gacgacggtg
      361 cgccgcctgc gccgccgccc gctgacgacg tcgacgaccg cgaagcgctg gtgctgttca
      421 ccagcggcac taccggactc ccgaagccga tcggcgtcac ccacaggcgg ttgagtgctc
      481 gaatctatgg agtcacaaca cctttccggg cggacgcacc gccagttgtg agcatgatga
      541 gcgtgccctt tttccacgtc ggcggcgcca tcggcgtgct cggaaacctg tactcgggca
      601 acacctacct ggtgcagaat cggttcgacg ccggagagtg gttgcgtctg gtgcaagcgc
      661 accgggtcac atccaccttc atggtgccga cgatgctgcg gcggatcctc gaccatccca
      721 acttcggcaa cacggacctc tcgtcgttga ctgccatcgc ttacggggcc gcggccgcac
      781 caatcgcgtt ggtgcgcagg gccatggacg ccatgccgca tgttggattc gccaacgtat
      841 ttgggcagac ggagaccttg ggcgcctata ccactctgct gccggccgat catcgcgacc
      901 ccaaccgcgc aggttccgtc ggccgtccac tacccggagt cgaggtccga gtggtcgacc
      961 ccgacaccgg gatcgacgtc gacgtcggga ccgtcggcga gttgtgggtg aacaccaccc
     1021 agaacgtcac cggcgaagac tggctgcata caggcgatct cgctcgtgag gatgccgacg
     1081 gctacatcta tccggtcggc cggttgaagg acaccatcaa ccgcggggga gagaagttcg
     1141 ggccgatcga gatcgaggac gccattcgga cccatccggc cgtgcgcgac gtggccgtcg
     1201 ccggaatcgc cgacgaggag atgggtcagc gggtaggcgc cgccgtcgtc gcccgctatc
     1261 cgctgaccct cgaggaactg cggtcgcact gccgcgagtt gatcgcctac ttcaagctgc
     1321 cggagcgcct gaagatggtc gaacgcatcc cgtacaccga gaccggcaag gtgaaccgcc
     1381 gtcagctcgc cgcattgatc gccgacgggg cctgagcgag aggagtcgcc gtgctgttcc
     1441 ttcacgaaac ccacaaggtg gtcggcgcgc gcgaggacga gttcgaggcc gcctaccgcg
     1501 agggctggat gccgacgctt gcgcaggggg aggacgcgcg acttctgtgg tacaccaacc
     1561 acgctcacgg ttccggggtg tcctacaacg tcgtcaccat cactgggatc gccgacggcg
     1621 cagcttggga acgcctcgcc ctgcgcgtcc aatcggggga cctgcaaccc tggatgcgtg
     1681 agctggacaa cctgcgtcac gaggtgaccg gcaagatctt gctgccggtc gagtggtcac
     1741 cccttcagga agtagatctg gccaccgttc cgactgatgg tgcagcgcat ccactgagcc
     1801 tcttcatgga ggacaccggc tggccctacg ctccgctgga cgactacatc cggtcgtggg
     1861 acgagatcta ctaccggccg ctgtccacga cgtcgcagat actcgacatc caggcctgct
     1921 tccaagtggc gcatggcagc taccgacggc gagaagccat gctgtggcag aaaatctcgc
     1981 cggccaacga ctacgccacg ctcgtgcacc tgctcacctc cgacatctca cctgagcatc
     2041 gcggcccggg cacgtacatg ttcgaggcgc tgaaataccg cgaccagtgg gagagcaggc
     2101 tcctgcggac ctcggcctgg tcaccgttgt actaaggcgc caagggagga ctagacggtc
     2161 agccgagaac gacgggaagt ttcgccgcga ggtcacctaa ttgagctttc tcagtgtcgg
     2221 tcggagtacg ccgcccggtg ccgatcagca gagcgtcctt gtcgggcaac agctttggga
     2281 atgcagcgcc gactatcgtc tcgagcatct cgcgcgaccg agcgaggccg tccgcgggcg
     2341 gttcggccac gccaggcaag tgtgcgatca gatcgaggtg gtgcagcgtc cactccagga
     2401 cataggccga gagatagtca g
//
LOCUS       DASRJA010000564        14050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4145220,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000564 DASRJA010000000
VERSION     DASRJA010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14050
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4145220"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..629
                     /locus_tag="VFC01_24840"
     CDS             324..629
                     /locus_tag="VFC01_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82877.1"
                     /translation="MRAILLTLIGLAAAACGLILAPSASAAESAIVTIGSLQAQGFDV
                     KIDRVGSAPLSECVVTNVRNPRNRTEFVPVIGDRDRGGVVPVIVDRTVTVSLDCSRR"
     gene            complement(692..1003)
                     /locus_tag="VFC01_24845"
     CDS             complement(692..1003)
                     /locus_tag="VFC01_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82878.1"
                     /translation="MRTKVMLAAVAVGVCGAIAAPAGLASADESAQQTISRLQSEGYT
                     VNVDRIGTGPIDQCVVTNVRNPQQVKQWVPYTGPGRDGDRVLVQVITSQTISVTLNCS
                     R"
     gene            complement(1158..2348)
                     /locus_tag="VFC01_24850"
     CDS             complement(1158..2348)
                     /locus_tag="VFC01_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HZN82879.1"
                     /translation="MPNKVFVVGVGMTKFEKPGRREGWDYPDMARESGTNALKDAGID
                     YSEVEQGFVGYVAGDSTSGNRALYELGMTGIPIVNVNNNCSTGSTALFLAAQAVRGGL
                     VDCAIALGFEKMQPGPLQGGAQDRESPMGKHVKAMSEIDEFAFPVAPWMFGAAGREHM
                     RQYGTTAEHFAKIGLKNHKHSVNNPYAQFQDEYSLDDILAAKMISDPLTKLQCSPTSD
                     GSGAAIVASEGFVDKHGLAPQAVEIVGQAMTTDFDSTFDGSAKNLIGYDMNVQAAQKV
                     YDQSGLGPSDFQVIELHDCFSANELLLYEALGLCGEGDAPKLIDNNDTTYGGRWVVNP
                     SGGLISKGHPLGATGLAQCAELTWQLRGTADKRQVDNVTAALQHNIGLGGAAVVTAYQ
                     RAER"
     gene            complement(2414..2731)
                     /locus_tag="VFC01_24855"
     CDS             complement(2414..2731)
                     /locus_tag="VFC01_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZN82880.1"
                     /translation="MNNNRTAPLQVPQPISAATARRRHGSGALLVDIRPQVARHQGTI
                     TGALIIDPAAIADQITDRRREIVVCSVSSQRAIPAAEQLSQLGYDRVHYLAGGYGAWT
                     ARK"
     gene            complement(2797..3138)
                     /locus_tag="VFC01_24860"
     CDS             complement(2797..3138)
                     /locus_tag="VFC01_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN82881.1"
                     /translation="MFATHRLTTFAGTALVAAALGLAAVATAGTAGALVSVDDAFLSE
                     ISSEGIAYDSPKAAVANAHAVCSALDDGADPVDVGQEILDNTDLTTHQAAVFVVSAVN
                     AYCAEYTVYFE"
     gene            complement(3249..5015)
                     /locus_tag="VFC01_24865"
     CDS             complement(3249..5015)
                     /locus_tag="VFC01_24865"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN82882.1"
                     /translation="MSDPDRESRVGSQIGPYRLRRLLGRGGMGEVYEAEDTVKDRVVA
                     LKLLPEAVSHDPVFRKRLQREAHSAGRLQEPHVVPIHDYGEVDGLLYVDMRMIDGTDL
                     RHMLKRYGPMTPARAVAIVRQVASALDAAHESGVMHRDVKPENILITRDDFAYLVDFG
                     IANAATDEKLTELGTAVGTYAYMAPERFTNDEVTYRADVYALTCVLHECLTGSQPYPG
                     DSVSVVITAHLMQPIPKPSAERPGIPGAFDTVIAKGMAKKPEDRFASAGDLAIAANEA
                     MSQRDQDKAATILQRSQDATMPGTPIPPMQPGRPIHQTPPPTPPPTPPPFAATPPPTP
                     PPFAATPPPTPPPARPVAFHTPPPPSGPRSGPMWTAVPPPPGPPRAKNKVPWIPIAAA
                     AGVLVLVLGGVGIWLMTKSDDTTSSTGPTTSATSETAEPTETTRRTPRTTTPTNEQSF
                     DSQLMAVLPRGYDDGVCQAVHPPATGALATVDCGQSSIAGGPANARYSLFADQQTLDT
                     HFDGAIKENSELFQCPGSGVDSPTTWHYTETPADVEGRIACGTYNNGPDLVWSKNSDL
                     MLADAQAPNIDDLHNWWLKYTR"
     gene            complement(5064..6185)
                     /locus_tag="VFC01_24870"
     CDS             complement(5064..6185)
                     /locus_tag="VFC01_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HZN82883.1"
                     /translation="MNKIGFGVLGPLQMTVDGTPVPLGTPKQRAVLAVLVMNRNRPVG
                     IDSLITAAWEQWPPSEARASLHSYISNLRRLLGDSRSMLVNAPPGYRLAVDDTACDIG
                     RFILEKSAGVQAAAAGHFEEASRHLTTALAEWRGPVLEDLRDFQFVDAFATALTEDKV
                     VAHTARAEAEIACGRGYAVIGELESLTVEHSYREPLWAQLITAYYLAERQSEALDAYQ
                     RLKTTLADDLGIDPGPTVRALHERILRQEPLDVKKAAKTTAVHTIHKIELRTSISPSH
                     AHASLRSTNGRVYPLHAAATRIGRLDDNDIVLDDANVSRHHAVIIDTGTSFVITDLRS
                     ANGVDVGNERIRGTATLANGDRIRICDHEFTFEIAPRET"
     gene            complement(6203..6922)
                     /locus_tag="VFC01_24875"
     CDS             complement(6203..6922)
                     /locus_tag="VFC01_24875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82884.1"
                     /translation="MRYSGSRAGLGVDLNADHLAVRRLDPHGNPVGQPKRIDLDLSGS
                     SYRRDAQIRHAITQLVHYTQQHNIGSVAVEDLDFTDARTTGRETMGRGRRGKRFRRAV
                     AGIPTTVFRNRLTAQTPRHGMALYAVNPAYTSAWSDEHWRTPYENVTRHEAAATVIGR
                     RAQGFKARRRNGVTRMRPADRVVRATDQAAPDDRQVSTRSRHRPGTRGTESRALGRAR
                     TRHLGRATVTPAPANNGYLRQ"
     gene            complement(7186..7896)
                     /locus_tag="VFC01_24880"
     CDS             complement(7186..7896)
                     /locus_tag="VFC01_24880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82885.1"
                     /translation="MAAVKLRQWRGVKPVGGPAGVAITTRFATTPEDERVLDLVADHQ
                     GRVRRADLATVARPEPMVPTHNATAQRQARRDRLNARKTALTAESSARWANAIIAAND
                     AQYRSARDAQHRHIIGLRAAIATIETRLAQPTADTVSPKQRRVRRKAKLPKGYATQAE
                     RFQKQRRLQMLRARLHRVQADFDDRIEHVVEGGKRPVDKRHHLDTANLTDLYGAKSGN
                     APATASKPSAQAMSRSGT"
     gene            8036..9085
                     /locus_tag="VFC01_24885"
     CDS             8036..9085
                     /locus_tag="VFC01_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN82886.1"
                     /translation="MGLRVVQWATGGVGVAAIKGVLEHPDLELVGCWVHSESKAGKDV
                     GDIIGIEPLGVTATNSVDEILALDADAVVYAPLLPNPDEVAALLRSGKNVVTPVGWVY
                     PSERQGAPLREAALEGNATLHGTGMAPGGISEKFPLMFSIMSTGVTFVRAEEFSDLRS
                     YEAPDVVRHVMGFGEVPEKALSGPMQKLLDGGFIQSVRICVDKLGFNADPKVRSTQEI
                     AVATAPIDSPIGVIEPGQVAGRKFHWEALVGDDVVVRVTVNWLMGEENLDPAWTFGPE
                     GQRYEIEVRGNPDISIVVKGFQSEIGGEGPEYGVVGTAAHCVNSIPAVCAAKPGIATY
                     LDLPLISGKAAPGLS"
     gene            complement(9097..9837)
                     /locus_tag="VFC01_24890"
     CDS             complement(9097..9837)
                     /locus_tag="VFC01_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN82887.1"
                     /translation="MTALTDRVALLTGASGGIGKGIARALAEEGVDLFLAYGRHGHDA
                     EEAATYARDLGRRAVIASADLADPAAPARLVAQANSELGVVDILVANAGTADVKGWQN
                     IDLESWNATLAVNLTAPFLLAQQVLPGMIERKFGRVLFISSIAGLNGGVVGAHYAASK
                     AGLHGLTHHLAARVAADGVTVNALAPALIGDTGMFPVDPETRTTPMPIPVGRAGRPAE
                     VADIAIAMLRNGYLTNKVVPLDGGILPR"
     gene            complement(9834..10877)
                     /locus_tag="VFC01_24895"
     CDS             complement(9834..10877)
                     /locus_tag="VFC01_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN82888.1"
                     /translation="MVDTMRAERFYADSKTIAVEDIPVPEPGPGEVLVKVAFCGICHS
                     DLSLINGTFPAQRPVVTQGHEASGTIAKLGPGVTGWAEGDRVIVAAGRPCTTCPNCRR
                     GDIANCQRIQLMAFAYDGAWAEYTVAQAVGLTRVPDNVPLEQAAILADAVSTPFGAVV
                     RTGQVGIGESVGVWGVGGVGTHIVQLARLVGAVPVIAVDIKPAVLDRALEMGADHALD
                     ASDPALREKIAEITGGRMLDVAFDAVGLKSTFEQALDCVTVGGRFVCVGMSAEAPSVG
                     PTNMFGLTQKHVLGHLGYQNVDIETLATLVSHGRLDLSRSISDIVSLEDVRVGIEKLD
                     RQEGNPIRILVKP"
     gene            10900..11769
                     /locus_tag="VFC01_24900"
     CDS             10900..11769
                     /locus_tag="VFC01_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HZN82889.1"
                     /translation="MCRLFGLHADKRVVTATFWLLEAPDNLVEQSKRNPDGTGLGVFD
                     PDGRPVVYKEPVAAWQDRQFATEAHQLTGTTFIAHVRYSSSAALETRNTHPFLQDDRI
                     FAHNGVVTGLDVVDERLHALGVGDLVLGDTDSERIFALITACTRRGGDVGAGIVDAVG
                     WLAETVPIFAVNMLLSTATDMWALRYPETHELYLLDRRKVLAPDQLFELSTDRIHARS
                     RHLMALPKVIFASEPMDDDPRWQLMEPGELAHVDADLQIDRRIALPDPPKHQLRQDDL
                     TARAAASQHASVE"
     gene            complement(11770..12498)
                     /locus_tag="VFC01_24905"
     CDS             complement(11770..12498)
                     /locus_tag="VFC01_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HZN82890.1"
                     /translation="MRTLAALVAAVTGMLIGFAPTALAEPPAPPAPIIPPPVQPANIG
                     DLKFTAIDYYNSGAYLTDLQIATAPAIAWITEEAPRVDRPAVVFDIDETALSNWEALK
                     ANDFGRIYNGGCDRLPHGPCGLIAWDQRAQSTVIQPTMNVFTTARDSGAAIFFITGRG
                     ESQRAATERNLQAVGYTGYTQLIMEPPGAHYISAADFKAPQRAQIEQQGYTIIANLGD
                     QPSDLDGGFAERTYQLPNPFYRIP"
     gene            12536..13393
                     /locus_tag="VFC01_24910"
     CDS             12536..13393
                     /locus_tag="VFC01_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZN82891.1"
                     /translation="MTTKRALVLAGGGLAGIAWETGILRGIADESPETAKAVLDADVL
                     VGTSAGSTVAAQLSSGLGLDALFDRQITATSAELNPNVEVEEIAELFLTAMVTPNTTK
                     AEKLQKIGAVALSTVTVTEGVRRDVIAQRLPSHDWPDRVLRISAIDTATGELVMFDRN
                     SGVGLVDAVAASCAVPGVWPPVTIGGRRYMDGGVGSSVNLALANDCDVAVVLVPSGRS
                     APSPFDSGAAEEVDAFGGSTFGMFADDESLTAFGRNPLDPACRKPSALAGREQGRRLA
                     GAITDFLGA"
     gene            complement(13395..>14050)
                     /locus_tag="VFC01_24915"
     CDS             complement(13395..>14050)
                     /locus_tag="VFC01_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZN82892.1"
                     /translation="VTIEQLPIPYTAVSTDLLSGKSVWLQRGPVDAAIRASIAIPGVI
                     TPHVFDGRLLADGGILDPLPMAPIAAVNADLTIAVSLAGSEAGGRYEPTETEPRATTE
                     WLSRMWRSTTALFESNRAVTPPDETASPDELVDTSKEEAVVPKLGSFEVMNRTIDIAQ
                     AALARHTLAAYPPDLLIEVPRTACRSLEFHRAAEVIDIGSELAAIALDELEKATPQS"
BASE COUNT         2174 a         4499 c         4782 g         2595 t
ORIGIN      
        1 cgttctgggt ggagaggacc cgttcgacga actcaccatg gtcgacgacg atcgcacctc
       61 gcacgccgaa gaaccggcac aggagcgtgt ccctgtgccg gcgtagggcg cgcgaacgaa
      121 aggcccggcc gaactccccc ccgtcggccg ggcctttctc atgcctcgac actgcggtgg
      181 gatcgcgaaa tcgcaagaaa tcgcgatctc acagcagttt cggcgacgtc tcgaagagtt
      241 tttgaaattc ctttgaaccc cctgcgacct ccgcccgttg aaccggctga catccactca
      301 acggaaagaa acggaggctc gccatgcgtg ccatcttgct cacactgatc gggttggccg
      361 ctgcggcttg cggtttgatc ctcgcccctt cggcctcagc ggccgagtct gcaatcgtca
      421 ccatcggctc gttacaggcc cagggctttg acgtgaagat cgatcgcgtc gggagcgcac
      481 cgctctccga gtgcgtggtc accaacgtcc gaaacccaag gaaccgaacg gaattcgtgc
      541 ccgtgatcgg cgaccgcgat cgtggcggcg tggtccccgt gatcgtcgac cgcaccgtca
      601 cggtgtcgct ggactgctcc cgacgctgac agtgcaaggc ccggccggag taacccggtc
      661 gggccttgcc gtgtgtggct gctgtgtcag ctcagcgcga gcagttcagg gtcacggaaa
      721 tcgtttggct cgtaatcact tgcacgagca cgcggtcgcc gtccctgccg ggcccggtgt
      781 agggcaccca ctgcttgact tgctgaggat tcctgacgtt cgtcaccacg cattggtcga
      841 tcggaccggt gccgatgcgg tcgacgttga cggtgtagcc ctcgctctgc agccggctga
      901 tcgtctgctg ggctgactcg tcggccgacg cgaggcccgc gggggcggcg atcgctccgc
      961 atacacccac agcgacagcg gcaagcatca cctttgtccg catggccgca cctcccgtcc
     1021 tttgcctagg tcaatactcc tccacctcaa cgacgcgggc cacctaattc gttcccgcgg
     1081 cagcgcgagc agacataaaa ggccccgaaa cgcccagaaa ttggggcctt ttgcgtctgc
     1141 tcgcgctagg agaaccctta ccgctcggca cgttggtagg ccgtcaccac cgctgccccg
     1201 ccgaggccga tgttgtgctg cagcgcggcg gtgacgttgt ccacctgccg cttgtcggcc
     1261 gtgccgcgca gctgccatgt cagctccgcg cattgcgcaa ggcccgtcgc acccagcgga
     1321 tggcccttgg agatcagccc gccggacgga ttgaccaccc aacggccgcc gtaggtggtg
     1381 tcgttgttgt cgatcagctt cggggcgtcg ccctcgccgc acagtcccag cgcctcgtac
     1441 aacagcagct cgttggcgga gaagcagtcg tgcagctcga tcacctggaa gtctgacggg
     1501 ccgagcccag actgatcgta aaccttttgt gcggcttgaa cattcatgtc atagccgatc
     1561 aggttcttgg cgctgccgtc gaaggtgctg tcgaagtcgg tggtcatcgc ttggccgacg
     1621 atctccaccg cctgaggagc cagcccgtgt ttgtcgacaa acccctcgct ggccacgatc
     1681 gccgcgcccg acccgtcgga ggtcggcgag cactgcagct tggtcagcgg atcggagatc
     1741 atcttcgccg ccaggatgtc gtcgagcgaa tactcgtctt ggaactgggc gtaggggttg
     1801 ttcaccgagt gcttgtgatt cttcagcccg atcttcgcga aatgctctgc ggtggtgcca
     1861 tattggcgca tgtgctcgcg acccgcggca ccgaacatcc acggcgccac cgggaatgcg
     1921 aactcgtcga tctcggacat cgccttgacg tgcttgccca tcggcgactc gcggtcctgg
     1981 gcgccgcctt gcagcgggcc gggctgcatc ttctcgaagc caagcgcgat cgcgcagtcg
     2041 acaagcccgc cgcggacggc ctgcgccgca aggaacaacg ccgtagaacc agtcgaacag
     2101 ttgttgttga cgttgacgat cgggatgccg gtcatgccga gctcgtagag cgcccgattg
     2161 ccggacgtcg agtcgccggc cacatagccg acgaagccct gctccacctc ggagtagtcg
     2221 atgccagcgt ccttcaacgc attcgtccct gattcacgcg ccatgtccgg gtagtcccag
     2281 ccctcgcggc ggcccggctt ctcaaacttc gtcatcccca cgccgacgac gaaaaccttg
     2341 ttcggcatcg cagtttccct tccctgttcc tgcgatcgac agaaacacaa agcgggcgaa
     2401 ctgtctagat gctctattta cgcgccgtcc aggcgccgta tccgccggcg aggtagtgca
     2461 cgcggtcgta gccgagctgg ctgagctgtt cggcggctgg aatggcgcgc tgcgacgaga
     2521 cgctgcagac cacgatttcg cgtcggcggt cggtgatctg gtcggcgatg gctgcggggt
     2581 cgatgatcaa cgccccggtg atggtgccct gatggcgggc gacctggggg cggatgtcga
     2641 cgagcagcgc gcccgatccg tgccggcgcc gcgcggtagc cgccgagatg ggctggggca
     2701 cctgaagcgg tgcggttcgg ttgttgttca tggtcgttct cttaggggtg gtgcgggggc
     2761 cccgccgccg cagcggggcc cccgcggggg gaggggttat tcgaagtaga cggtgtactc
     2821 ggcgcagtag gcgttcaccg cggagaccac gaagacggcg gcctgatggg tggtcagatc
     2881 cgtgttgtcc aggatctcct ggccgacgtc cacggggtcg gcgccgtcat ccagggcgct
     2941 gcacacggcg tgagcgttgg cgacggccgc cttcgggctg tcgtacgcga tgccctcgct
     3001 gctgatctcg ctcagaaacg cgtcgtccac cgagacgagg gcgccggcgg tgccggcggt
     3061 agcaacggcg gccagtccga gggcggccgc gacgagagcg gtgccggcga acgtggtgag
     3121 gcgatgcgtg gcgaacattg gactccttgg tgatgtcgtt ttgttccggg tgtttccggg
     3181 ttgacatcag agtccgatag cggtcttggc ggattcttga aagattcttg gcggttgtga
     3241 cgtcgttctc agcgggtgta cttcagccac caattgtgca ggtcatcgat gttgggcgcc
     3301 tgcgcgtcgg cgagcatcag gtccgagttc ttggaccaca ccaggtcggg tccgttgttg
     3361 taggtaccgc acgcgatgcg gccctcgacg tcggcgggcg tttcggtgta gtgccacgtg
     3421 gtcggcgagt cgacgccgct gcccggacat tggaacagct cggagttctc cttgatggcg
     3481 ccatcgaagt gggtgtccag cgtctgctga tcggcgaaca acgaataccg ggcattcgcc
     3541 gggccgcctg cgatcgatga ctggccgcag tcgaccgtcg ccaacgcgcc ggtggccggc
     3601 ggatgcacgg cctgacacac gccgtcgtcg tagccgcgtg gtagcaccgc catgagctgg
     3661 gagtcgaacg attgctcgtt ggtgggcgtc gtcgttctag gggtgcgcct cgtcgtttcc
     3721 gtcggctcag cggtctccga ggtggcgctg gtcgtcggtc ccgttgacga cgtcgtgtcg
     3781 tcggacttcg tcatcaacca gatgcccacc ccgccgagga ccaggacgag gacgcctgcc
     3841 gccgcagcga tcggtatcca cggaaccttg ttcttggcgc gcggtggtcc gggcgggggc
     3901 gggacggccg tccacatcgg cccgctgcgc ggcccactcg gcggcggcgg ggtgtggaag
     3961 gcgacgggtc gggcgggcgg cggtgtcggt ggaggcgtcg ccgcgaacgg cggtggggta
     4021 ggtggtggcg tcgccgcgaa cggcggtggg gtaggtggtg gcgtcggcgg cggcgtttgg
     4081 tgaatcggtc ggccgggctg catggggggt atcggcgtgc ccggcatcgt cgcgtcctga
     4141 ctgcgctgca ggatcgtcgc ggccttgtcc tgatcccgtt ggctcatcgc ctcgttcgcg
     4201 gcgatcgcta ggtccccggc gctggcgaat cgatcctccg gcttcttcgc catccccttg
     4261 gcgatcaccg tgtcgaaggc gcccggaatg ccagggcgct ccgcgctcgg tttagggatc
     4321 ggctgcatca gatgcgcggt gatgaccacg ctgacgctgt cgcccggata cggctgcgat
     4381 ccggtaaggc attcgtggag gacacaggtc agcgcgtaca cgtcggcgcg gtaggtgact
     4441 tcgtcgttgg tgaaccgttc cggcgccata tacgcgtagg tccccaccgc ggtgcccaac
     4501 tccgtcagct tctcgtccgt ggccgcgttg gcgataccga agtcgacgag atacgcgaag
     4561 tcgtcgcggg tgatcagaat gttctcgggt ttgacgtcgc ggtgcatgac gccgctctcg
     4621 tgcgcggcat ccagcgccga ggcgacctgt cgcacgatcg ccacggcccg cgccggcgtc
     4681 atcggcccgt accgcttgag catgtggcgc agatccgtgc cgtcgatcat tcgcatgtcg
     4741 acgtagagca gcccgtcgac ctcgccgtag tcgtggatcg gcaccacatg gggttcctgc
     4801 agtcgccccg ccgaatgggc ttcgcgctgc agccgctttc ggaacaccgg gtcgtgagag
     4861 accgcttccg gcaagagttt cagcgcgacg acgcggtcct tgacggtgtc ctcggcctcg
     4921 tagacctcac ccatgccgcc cctgccgagg agccgccgca agcgatacgg accgatctgg
     4981 gaacccacac gtgattcccg gtcggggtcg ctcatcggtt actcctcgga tgcgtttgcc
     5041 agcgaacaca ccctaatgcc gcactacgtc tcacgcgggg cgatctcgaa cgtgaactcg
     5101 tggtcacaga tgcggatccg atcgccgttc gccagcgtcg ccgtgccgcg gattcgttcg
     5161 ttgcccacgt cgactccgtt cgccgagcgc aggtcggtga tgacgaaact tgtgccggtg
     5221 tcgatgatga cggcgtggtg gcgactgacg ttggcgtcgt cgagcacgat gtcgttgtcg
     5281 tcgagccgcc cgatccgagt ggcggcggca tgcaagggat acacccgccc gttcgtcgaa
     5341 cgcagactcg cgtgcgcgtg cgaagggctg atcgacgtgc gcagttcgat cttgtggatg
     5401 gtgtgcactg cggtcgtctt ggccgccttc ttcacgtcga gcggctcctg gcgcaggatc
     5461 cgttcgtgta gggcacgcac cgtggggccc gggtcgatgc ccaggtcatc ggcgagcgtg
     5521 gtcttcagcc gctggtaggc gtcgagcgcc tccgattggc gttcagccaa gtaataggcg
     5581 gtgatcaact gcgcccacaa cggctcgcga taggaatgtt cgaccgtcaa cgattcgagc
     5641 tcgccgatga cggcgtaccc acgcccgcaa gcgatttcgg cttccgcccg agcggtgtgg
     5701 gcgaccacct tgtcctcggt cagggcggtg gcgaacgcgt cgacgaactg gaagtcgcgc
     5761 agatcctcca gtaccggtcc gcgccattcg gccagcgcgg tggtcaggtg gcggctggct
     5821 tcctcgaaat ggcccgcggc ggcggcctgt acgcccgcgc tcttctcgag gatgaatcgg
     5881 ccgatgtcgc aggcggtgtc gtcgactgcg agtcggtatc ccggtggcgc gttgaccaac
     5941 atcgaccgtg agtcgccaag cagcctgcgc aggttcgaga tgtaggaatg caggctggcc
     6001 cgagcttccg acggcggcca ctgttcccac gccgcggtga tcagcgagtc gatcccgacc
     6061 ggccggttgc ggttcatgac cagcactgcc agcacggcgc gttgtttcgg ggtgccaagc
     6121 ggcacaggcg tgccgtcgac cgtcatctgc agcggcccga gcacaccgaa accgatcttg
     6181 ttcatcaggc caattgtcac cgttactgtc ttagatagcc attgttggcc ggtgccgggg
     6241 taacggttgc ccggcccagg tgccgcgttc ttgcccggcc gagcgcgcgg gattcggttc
     6301 cccgcgtccc tggccggtgg cggcttctgg tactcacctg tcggtcatcg ggcgcagcct
     6361 ggtcggtagc tctcacgacg cgatccgctg gtcgcatgcg tgtcacacca ttccgtcgcc
     6421 gcgccttgaa accttgggcg cgtctgccga tcacggtggc ggctgcctcg tgtcgggtga
     6481 cgttctcata tggagtgcgc caatgctcat cactccatgc gctggtgtag gcggggttga
     6541 cggcatacaa cgccatgcca tgtctagggg tttgggcggt gagcctgttg cgaaataccg
     6601 tcgttggaat tccggccacc gctcgacgga accgtttgcc gcggcgccca cgtcccatgg
     6661 tttcgcggcc cgtggtgcgg gcgtcggtga agtcgaggtc ctccaccgcg acagagccga
     6721 tgttgtgctg ctgggtgtag tggacaagct gggtaattgc atgccggatc tgggcatcgc
     6781 ggcgatacga cgaccccgac aggtctaggt ctatccgctt gggttggccg accgggttgc
     6841 cgtgcgggtc gaggcgccgc acggcgaggt ggtcggcgtt gaggtccacc cccaaaccgg
     6901 cccggctacc cgaatatcgc attcgcgggt ctcagggtcg cggtcgccgg gtggccatgc
     6961 gagcgaccca tgaggcggtg aggtagcggc cggcgcggcc cggtttgcga gtgatcgtgt
     7021 aggacacagg cttaccgctg gtgatcctgc ctcgccattc atcggcgcgg tagcgaaaca
     7081 ccgcctggcc ggaaaacacg tagcggccgt gcttggcgtt ggcgaggtgc tccaactgtt
     7141 ttggcagccg caggctgagt tcgccccccg cggtgaccgt gatggttaag ttcccgaacg
     7201 gctcatcgcc tgagccgagg gcttcgatgc ggtcgcgggc gcattcccac ttttcgcgcc
     7261 atataaatcg gtcaggttcg cggtgtcgag gtgatggcgc ttgtcgaccg gtcgtttgcc
     7321 gccctcgacg acgtgctcga tccggtcgtc gaagtcggct tgaacgcggt gcagtctggc
     7381 acgcagcatt tgcagccggc gctgcttctg gaatcgctct gcctgcgttg catagccttt
     7441 cggtagcttg gccttcctgc gcaccctgcg ttgcttgggg ctgacggtgt cggcggtggg
     7501 ttgggctaac cgggtttcga tggtggcgat cgctgccctc aggccaatga tgtggcgatg
     7561 ctgggcgtcg cgagccgacc ggtactgggc gtcgttggcg gcgataatcg cgttggccca
     7621 ccgcgcgctt gactcggcgg ttaacgcggt cttgcgggcg ttaagccggt cacgacgcgc
     7681 ttgccgctgg gcggtcgcgt tgtgggtcgg caccattggc tccggtcgag cgaccgtggc
     7741 cagatctgcc cgccgcaccc ggccctgatg gtcggcaacc aagtcgagca cccgctcgtc
     7801 ttcgggtgtg gtggcgaacc gggtggtgat cgccacccca gctggaccgc cgactggctt
     7861 caccccacgc cactgccgca acttgaccgc cgccacggcg ccgatcatgc cgcacacgca
     7921 cgacaaatcc agtcagtcga gtactggcga aaagaatcct gagtttctgg ttcgctcccg
     7981 gtgcgttgtg gtgtagaccg gagtagaacg tgttctagtt ttcagaggag atgacatggg
     8041 cctgcgggtc gtgcagtggg cgacgggtgg cgtcggcgtc gccgccatca agggcgtgct
     8101 ggagcatccc gacctcgaac tcgtcggctg ttgggtgcac tcggagagca aggcgggcaa
     8161 ggacgtcggc gatatcatcg gcatcgaacc gctgggtgtc accgccacca acagcgtcga
     8221 cgagatcctg gccctggacg ccgacgccgt ggtgtacgca ccgctgctgc ccaaccccga
     8281 cgaggtcgcg gcgctgctgc ggtccggcaa gaacgtagtg accccggtgg gttgggtcta
     8341 tcccagtgag cggcagggtg cgccgctgcg ggaggccgcg ttggagggca acgcgacgtt
     8401 acatggcacg gggatggcgc ccggcggtat cagcgagaag ttcccgttga tgttctcgat
     8461 catgtcgacg ggcgtgacat tcgttcgtgc cgaggagttt tccgatctgc gtagctacga
     8521 ggcccccgat gtggtccggc acgtgatggg cttcggcgag gtcccggaga aggcgttgtc
     8581 cgggccgatg cagaagctgc tcgacggcgg cttcatccag tcggtccgga tttgtgtcga
     8641 caagctgggc ttcaacgccg acccgaaggt gcggtcgacg caggagatcg cggtggcgac
     8701 ggccccgatc gactcaccga tcggggtcat cgagccggga caggtcgcag gccggaagtt
     8761 ccactgggag gccctggttg gcgacgatgt cgtggtccgc gtgacggtga actggttgat
     8821 gggtgaggaa aacctggacc ccgcatggac attcggcccc gaagggcagc gctacgagat
     8881 cgaggtccgc ggcaaccctg acatttcgat cgtggtcaag ggttttcagt ccgagattgg
     8941 tggcgaaggc cccgaatacg gcgtggtggg caccgccgca cactgcgtga attcgattcc
     9001 cgcggtgtgt gcggccaaac cagggatcgc cacgtatctc gatctgccgt tgatcagcgg
     9061 caaggcagcg ccaggactga gctaacacaa gccgccttag cgcggcagga ttccgccgtc
     9121 caacggcacc actttgttgg tcagataccc gttgcgcagc atggcaattg cgatgtccgc
     9181 tacctccgcg ggccggccgg ctcggccgac cggaatgggc atcggcgtgg tgcgcgtctc
     9241 cggatcgacg gggaacatcc cggtgtcacc gatcagcgcc ggagccaacg cgttgacggt
     9301 gacgccgtcg gcggccaccc gcgccgccag atgatgcgtc aacccgtgca atccggcttt
     9361 gctcgcggcg taatgcgccc ccacgacgcc gccgttgagc cctgcgatcg acgagatgaa
     9421 caacacgcgc ccgaactttc gctcgatcat gccgggcaac acctgctggg ccagcaggaa
     9481 cggcgcggtc aggttgaccg ccaacgtggc attccaggac tcgagatcga tgttctgcca
     9541 accctttacg tccgctgttc ctgcgttggc gaccaggatg tcgacgacgc ccagttcgct
     9601 attggcctgc gcgacgagac gcgcgggcgc ggcggggtcg gctaggtccg ccgatgcgat
     9661 gacggcgcgc ctgccgaggt cgcgcgcgta ggtggcggcc tcctcggcgt cgtggccgtg
     9721 gcgcccatag gccagaaaca ggtcgacgcc ctcctcggcg agggcgcggg cgatgccctt
     9781 gccgatgccg ccggacgcgc cggtcagcag ggccaccctg tcggtcaagg ccgtcacggc
     9841 ttcaccagaa tccgaatcgg gttgccctcc tggcggtcca gcttctcgat gcccaccctg
     9901 acgtcttcga gggagacgat gtcgctgatg gaccgcgaca ggtccagccg cccgtgcgac
     9961 acaagcgtgg cgagggtttc gatgtcgacg ttctggtacc cgagatggcc cagcacgtgc
    10021 ttctgcgtga gtccgaacat attggttggc ccaacgctcg gagcctcggc gctcatcccc
    10081 acacagacga agcgcccgcc aactgtgacg caatcgaggg cctgctcgaa tgtcgacttg
    10141 agcccgaccg cgtcgaacgc cacatcgagc atccggccgc cggtgatctc ggcaatcttt
    10201 tcccgcagcg cggggtcgct cgcgtccaac gcgtggtcgg cgcccatctc gagtgcccga
    10261 tccaacacgg cgggcttgat gtcgacggcg atcaccggca ccgcgccgac taaccgcgcc
    10321 agctgcacga tgtgcgtgcc gaccccgccg acgccccaca ccccgaccga ctcaccgatg
    10381 cctacctgcc cggtacgcac tacggcgccg aatggcgtcg acaccgcatc ggccaggatg
    10441 gcggcctgct ccagcggaac attgtcaggc acccgggtca ggccaaccgc ctgcgcgacg
    10501 gtgtactcgg cccatgcgcc gtcgtaggcg aacgccatca actggatccg ctggcaattg
    10561 gcgatgtcgc cacgccggca gttcgggcac gtcgtgcagg gccgtcccgc ggcgacgatc
    10621 acccggtcgc cttcggccca cccggtgact ccggggccga gcttggcgat cgtcccagac
    10681 gcttcgtggc cctgcgtcac caccggtcgc tgcgccggga aggtgccgtt gatcaggctc
    10741 aggtcggaat ggcagatgcc gcagaacgcg accttgacga gcacctcgcc gggaccgggc
    10801 tcgggtaccg gaatatcttc caccgcaatg gttttggagt cagcgtagaa gcgttcggcc
    10861 cgcatggtgt ccaccattcc gccacgctac catcggacca tgtgtcggct attcggtctg
    10921 cacgccgata aacgggtggt gaccgcgacc ttctggctgc tcgaggcgcc cgacaatctc
    10981 gtcgagcaga gcaagcgcaa tcccgacggc accgggctgg gggtgttcga tcccgacggc
    11041 aggccggtgg tgtacaagga acccgtcgcc gcctggcagg accgccaatt cgcaacggaa
    11101 gcacaccagt tgaccggcac gacgttcatc gcgcatgtcc gctactcgtc gagcgccgcg
    11161 ctggagaccc gcaacacgca cccgtttctg caggatgacc ggatcttcgc ccacaacggc
    11221 gtggtgaccg gtctcgacgt ggtcgacgag cggctacacg cgctcggcgt cggcgatttg
    11281 gtgctgggcg acacggattc ggagcggatt tttgcgttga tcaccgcctg cacccgccgt
    11341 ggtggcgatg tcggcgccgg catcgtcgac gcggtcggct ggctcgccga gaccgtgccg
    11401 attttcgccg tcaacatgct gctgagcacc gcgaccgaca tgtgggcgct gcgctatccg
    11461 gaaacccacg agctgtatct gctcgaccgc cggaaagtgt tggcgccgga ccagctgttc
    11521 gagttgagca ccgaccgcat tcatgcccgc agccggcacc tgatggcgct gccgaaagtg
    11581 atcttcgcca gcgagccgat ggatgacgat ccgcgctggc agctcatgga accgggggag
    11641 ttggcgcatg tcgatgccga cctgcagatc gaccgccgca ttgcccttcc ggatccgccc
    11701 aaacatcaac tgcgccaaga tgatctgaca gcgcgggccg ctgcgtcgca acacgcctcc
    11761 gtcgagtgat cacgggatcc ggtagaacgg gttgggcaac tggtaggtgc gctcggcgaa
    11821 tccgccgtct aggtcggacg gctggtcgcc gagattggcg atgatggtgt agccctgctg
    11881 ctcgatctgg gcccgctgcg gcgccttgaa gtccgctgcc gaaatgtaat gggcgccagg
    11941 tggttccatg atcaactggg tgtagccggt gtagccgaca gcctgcagat tgcgctcggt
    12001 cgcggcgcgc tggctctcgc cgcggccggt gatgaaaaag atcgccgcgc cactgtccct
    12061 ggcggtggtg aaaacgttca tcgtaggctg gatcaccgtc gactgtgcgc gctgatccca
    12121 ggcgatcaat ccgcagggtc cgtgcggcaa tctgtcgcat ccgccgttgt agatacgacc
    12181 aaagtcgttg gccttgaggg cttcccagtt cgacagcgcg gtctcatcga tgtcgaacac
    12241 caccgccggt cggtccaccc gcggcgcctc ctcggtgatc cacgcgatcg ccggggcggt
    12301 cgcgatctgc agatcggtca ggtacgcacc gctgttgtaa tagtcgatcg ccgtgaactt
    12361 cagatcgccg atgttggccg gctgcaccgg cggcgggatg atcggcgccg gtggtgcggg
    12421 cggctcggcg agtgcggtgg gcgcgaaccc gatcagcatc ccggtgacgg cggcgaccag
    12481 cgcggcaaga gtacgcatga ttggacccta acggcggcct ggcacactcg ctgccgtgac
    12541 aaccaaacga gcgttggtgc tggccggcgg gggattggcg ggcatcgcgt gggagacggg
    12601 catattgcgg ggcatcgccg acgagtcacc cgagaccgct aaggccgtgc ttgacgccga
    12661 cgtgctggtg ggcacgtcgg ccggctcgac ggtcgccgct caactgagca gcgggctggg
    12721 actcgacgcg ctgttcgatc gccagatcac cgcgacgtcg gccgagctca atccgaacgt
    12781 ggaagtcgag gagatcgccg aactcttcct caccgcgatg gtgacgccga acacgacgaa
    12841 ggccgagaag cttcagaaga tcggcgccgt ggcgctgtct acggtgaccg tcacggaagg
    12901 ggtgcgccgg gacgtcatcg cccaacggct gccatcgcat gactggccgg atcgcgtgct
    12961 gcggatctcg gcgatcgaca ccgccacggg tgaactggtc atgttcgacc ggaactcggg
    13021 cgtcggcttg gtcgacgcgg tcgctgccag ctgcgcggtg cccggcgtgt ggccgcccgt
    13081 gacgatcggt ggccgccgct acatggacgg cggcgtcggt agcagcgtca acctggccct
    13141 ggcgaacgac tgcgatgtcg cggtggtgct ggtgccgtcg ggcaggtctg caccgtcccc
    13201 gttcgactcg ggcgcggccg aggaggtcga cgcgttcggc gggtcgacct ttggtatgtt
    13261 cgccgacgac gagtcgctca ccgcgttcgg ccgcaatccg ctcgaccccg cgtgccggaa
    13321 gccatccgca ctggccggcc gtgagcaagg tcggcggctc gcaggcgcca tcactgactt
    13381 cctcggcgcc tgaatcagct ttgtggcgtt gccttttcga gttcgtcgag cgcgatggcg
    13441 gccaactcgg acccgatatc gatcacttcg gccgcacggt gaaattccag gcttcggcat
    13501 gcggtgcggg gcacctcgat gagcaggtcg ggtggatacg ccgcgagcgt atgacgcgcc
    13561 agcgcggcct gcgcgatgtc gatggtgcgg ttcatcacct caaagctgcc gagcttgggc
    13621 accaccgcct cctctttgga cgtgtcgacg agttcgtcgg ggctggctgt ctcatctggt
    13681 ggcgtgacgg cgcggttgga ctcgaacaac gcggtggtgc tgcgccacat cctgctcaac
    13741 cactccgtcg tcgcccttgg ctcggtctcg gtcggctcgt agcggccgcc tgcttcgctg
    13801 cccgcgaggc tgaccgcgat ggtgaggtcc gcgttgaccg ccgcgatcgg cgccatcggc
    13861 agtgggtcga ggatgccgcc gtcggcaagc aagcgcccgt cgaatacgtg cggtgtgatc
    13921 acgccgggga tcgcgatgga cgcccggatc gcggcgtcga ccggaccgcg ttgcagccaa
    13981 acggatttgc ccgagagcag gtcggtggag acggcggtgt aggggatcgg cagctgttcg
    14041 atcgtcactt
//
LOCUS       DASRJA010000565         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1447439,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000565 DASRJA010000000
VERSION     DASRJA010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447439"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VFC01_24920"
     CDS             <1..770
                     /locus_tag="VFC01_24920"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HZN82893.1"
                     /translation="RPAARAFAVAAATTMLTALIGAAALAQTPPAPPAPAAPAPAAPK
                     AAPKAAPKAAPAPKPAETPAPQAQAPGGDQPQLIFSPWTKFCLKGQEANAKQVCFTGK
                     DGRVESGMPVVAAVLIEPENDPKKVLRVTLPLGMSLQPGTRVIVDQGQPMTGPYVICF
                     NNGCMADYEASGELIGKLKKGQGLVIQGINGAGQPISLVVPLQDFAKAYDGPPTDPKK
                     FEEQQKQLQDELQKRAEEARKKLEGQQQQQPPAAPTR"
     gene            complement(892..>3510)
                     /locus_tag="VFC01_24925"
     CDS             complement(892..>3510)
                     /locus_tag="VFC01_24925"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN82894.1"
                     /translation="SILATRVATGFGQPVFAGAAPGDTGRLFVVEKTGLIKILDLATG
                     AVLATPFLDLTGQIRTEGERGLLGLAFDPDFATNGFFYVYLINNPNADAEIRRYHVSS
                     DPNVADPLSVTPIITIDQTTNNNHKSGWIGFGPDGYLYIASGDGAPSTNGQDLNVLLG
                     KILRIDVHNDGFPADLARNYAVPADNPFATTTGADEIFALGLRNPWRDSFDRGLGDFY
                     IADVGANTWEEVNFGQSGANYGWNAFEGPDVFPGGGPLSTPSAVFPIYYYSHTVGQSI
                     TGGYVYRGEAEALQGQYFFADFVQGKIFTLRLDGTSWVATERTSQVTPDFGAINNPSS
                     FGEDARGNLYLVDFDGEVFKLTPTGASADQADTLQGFGGNDLLFGGSGNDTLDGGAGA
                     DTMLGGAGSDTADYSSSPVGVTVNLASGLGAGGDAQGDVLGGIENIIGSAHEDVLIGD
                     SGANTLTGGAGVDAMYGANGNDAYFVDNISDAVIENLNEGNDTVHASVNYRLGVNVES
                     LVLQGSAVQGYGNALSNMINGTSADNLLDGDTGADAMYGGAGNDAYFVDTTSDQVIEN
                     SGEGNDTVYAPVNYRLGANVEYLVLQGSAVQGYGNALSNVISGTSADNLLDGDAGVDA
                     MYGGAGNDAYFVDNINDVVVENLNEGNDTVYASINYRLGTNVEYLVLQGSAVQGYGNA
                     LSNVISGTGADNLLDGDAGVDAMYGGAGNDAYFVDNNGDVVIENLNEGNDTVYASIDY
                     RLGANVENLVLQGGAIQGYGNGLANALSGTSDANLLNGGGGGDFLTAGDGNDFFVFNA
                     GEANGDIVADFAGNGAGAGDFLVFVGFGTTVQGATFTQMGTSNQWQIHSGLDAHNEII
                     TFSNGASIDPTDFAFA"
BASE COUNT          642 a         1120 c         1109 g          639 t
ORIGIN      
        1 cccggccggc cgcacgcgcg tttgccgtcg cagcggcaac gacgatgctc accgcgctga
       61 ttggtgcggc cgccttggcg cagaccccgc cggcgccacc ggcacccgcc gcgccggcac
      121 ctgccgcgcc aaaagctgcc cccaaggccg cgcccaaagc agcgccggcg ccgaagccgg
      181 ccgagacacc cgccccgcag gcgcaggcac ccggcggcga tcagccgcag ctcatctttt
      241 cgccctggac caagttctgt ctgaagggcc aggaggccaa cgccaagcag gtctgcttca
      301 ccggcaagga tggccgagtc gagtcgggca tgccggtcgt ggccgccgtc ctgatcgagc
      361 cggagaacga tcccaagaag gtgcttcgcg tcaccttgcc cctgggcatg tcgctgcagc
      421 ccggaacgcg cgtgatcgtc gaccaaggcc agcccatgac cgggccctac gtgatctgct
      481 tcaacaacgg ctgcatggcc gactacgagg cgagcgggga gttgatcggc aagctgaaga
      541 aagggcaggg cttggtcatc cagggcatca acggcgccgg ccagcccatc agcctcgtgg
      601 ttccgcttca ggacttcgcc aaggcctacg atggcccgcc gaccgacccc aagaaattcg
      661 aggagcagca gaagcagctg caggacgagc tgcagaagcg ggccgaggag gcgcgcaaga
      721 agctcgaggg ccagcaacag cagcagccgc cagcggctcc gaccagataa cccttcgcaa
      781 tttggttctg atgagatcga ggcgcgcccc gggcgcgcct ctttctcttt catgagtgga
      841 agtcaagcgg ttgtacgcgc ggcacggcag accgaccatg ttgccgccgc ttcaagcgaa
      901 cgcgaagtcg gtcggatcga tcgaagcacc gttgctgaag gtgatgatct cgttgtgggc
      961 gtcaaggccg gagtggatct gccattgatt gctcgtgccc atctgggtga atgtggcccc
     1021 ttgcacggtg gtgccgaagc cgacgaatac gaggaaatcc ccagcgccgg cgccgttgcc
     1081 ggcaaagtcg gcgacgatgt cgccgttggc ttccccggcg ttgaacacga agaaatcgtt
     1141 gccgtcgccg gcggtgagaa agtcgccacc cccgccgcca ttgaggaggt tggcatcgga
     1201 ggtgccgctg agcgcgttcg ccagaccgtt gccgtagccc tgtatggcgc ctccttgcag
     1261 cacgagattt tccacgttcg ctcccagccg atagtcgatc gacgcataga ccgtgtcgtt
     1321 gccctcgttg agattctcga tcaccacatc gccgttgttg tcgacgaaat atgcgtcgtt
     1381 gccggccccg ccatacatcg cgtcgacgcc ggcatcgccg tcaagcaggt tgtccgcacc
     1441 agtaccgctg atcacgttcg atagagcgtt accgtagcct tgcacggcgc tgccctgcag
     1501 caccaggtat tccacgttcg tccccagccg gtagttgatc gacgcataga ccgtgtcgtt
     1561 gccctcgttg aggttctcga ccaccacatc gttgatgttg tcgacaaaat acgcatcgtt
     1621 gccggccccg ccgtacatcg cgtcgacgcc ggcgtcgccg tcgagcaggt tgtccgcact
     1681 ggtgccgctg atcacgttcg acagagcgtt accgtagccc tgcacggcac tgccctgcag
     1741 taccaggtat tctacgttcg cccccagccg atagttgacc ggcgcataaa ccgtgtcgtt
     1801 gccctcgccg gagttctcga tcacctggtc gctcgtagtg tcgacgaaat acgcgtcgtt
     1861 gccagctccg ccatacatcg cgtcggcgcc ggtatcgcca tcgagcaggt tgtccgcact
     1921 cgtgccgttg atcatgttcg acagagcatt gccgtagccc tgaacggcgc tgccctgcag
     1981 caccagggat tccacgttca ctcccagccg atagttgacc gaggcatgga ccgtgtcgtt
     2041 gccctcgttg aggttctcga tcaccgcatc gctgatgttg tcgacgaaat atgcgtcgtt
     2101 gccgttcgcg ccgtacatcg catcgacgcc ggcaccgccg gtgagcgtgt tggcgccgct
     2161 gtcgccgatg agcacgtctt cgtgcgcact gccgatgatg ttctcgatgc caccgaggac
     2221 gtcgccctgg gcatcgccgc cggcgccgag gccgctcgct aggttcaccg tcacgcccac
     2281 cggggaggag gaataatccg ccgtatccga gcccgcgccg cccagcatgg tgtcggcgcc
     2341 ggcgccgccg tcgagcgtgt cgttccccga cccaccgaac agcaggtcat tgccgccaaa
     2401 gccctgaagc gtatccgcct ggtcggcgga agcgccggtg ggcgtcagct tgaagacctc
     2461 gccgtcgaag tcgaccagat aaagattgcc gcgggcgtct tcgccaaacg acgatggatt
     2521 gttgatggcg ccgaaatcgg gggtgacttg cgaggtgcgt tcggtggcga cccacgatgt
     2581 gccgtcgaga cgcagagtga aaatcttgcc ttgcacgaag tcggcgaaga aatactgccc
     2641 ctgtagcgcc tccgcttcgc ctcgatagac gtagccgccg gtgatcgact gccctaccgt
     2701 gtggctatag tagtaaatgg ggaagaccgc cgatggggtc gaaagtggac cgccgccagg
     2761 gaagacatcc ggtccctcga acgcgttcca accgtagttg gcgccgctct ggccgaaatt
     2821 gacctcctcc cacgtattgg ccccgacgtc ggcgatgtag aagtcgccga gtccgcggtc
     2881 gaagctgtcc cgccacgggt tgcgcaagcc gagtgcgaat atctcgtcgg cgccggtcgt
     2941 cgtcgcgaag ggattatcgg ccggcaccgc atagttgcgc gcaagatcgg cggggaagcc
     3001 atcattgtgc acgtcgatgc gcaggatctt gccgagcaag acattgagat cctgcccatt
     3061 ggtggatggg gcgccgtcac cggacgcgat atagaggtag ccgtcgggtc caaaaccgat
     3121 ccagccagac ttgtggttgt tgttggtggt ctggtcgatg gtgataatcg gtgtgacgct
     3181 tagggggtcg gcgacgtttg ggtccgacga cacgtgatag cgacggattt cggcgtcagc
     3241 attggggtta ttgatgagat agacgtagaa aaaaccgttg gtagcgaagt caggatcgaa
     3301 agcgaggccg agcaggccgc gctcaccttc ggtccgaatc tgacccgtca ggtcgaggaa
     3361 cggcgttgcc agaaccgctc ctgtggcaag atcgagaatt ttgatcagcc cggttttctc
     3421 cacgacgaac agacggccgg tatcgcccgg tgccgcgccc gcgaaaactg gttgcccgaa
     3481 accggtcgcg acccgggtgg cgaggatgct
//
LOCUS       DASRJA010000566         4958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_309665,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000566 DASRJA010000000
VERSION     DASRJA010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309665"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1268)
                     /locus_tag="VFC01_24930"
     CDS             complement(111..1268)
                     /locus_tag="VFC01_24930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82895.1"
                     /translation="MWAKISTSATAMACAACIATAATLPTMGPAAEQLAKTASVKLSA
                     LTSLSASPLYEAFLANPNATTLGALLDGVYGTSATTLYTDFLADPSETTLSALLTVVG
                     ASTLGAVPLYADFLADPSETTLGSLLDGVKATSATTLYTDFLADPNETTLRALLAVVG
                     APTLGAVPLYAGFLADPSETTLGALLDGVKATSATTLYTDFLADPNETTLGALLAVVG
                     APTLGAVPVYLKALSDPSALAGINGLSALPDYLGIDPPEAAPDILNVAVTKATLAQES
                     ASTSSTPTTDSNPSVVQAASAALPDQLAPAPPVEPAVEATEVAPTVADNSGLDSQNIT
                     RNSLKFTPGSIGDSPILFGSGTPGGEGMRGYGSFLKKIGISAGEESSSGTG"
     gene            complement(1752..3728)
                     /locus_tag="VFC01_24935"
     CDS             complement(1752..3728)
                     /locus_tag="VFC01_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN82896.1"
                     /translation="MARHARSSRYSAYTGGPDPLAPPVDLREALEQIGQDVMEGSSPR
                     RALSELMRRGTRNMRGADRLAAEANRRRRELLQRNNLDGTLQEIKKLLDEAVLAERKE
                     LARALDDDARFNEMRIESLPASPAKAVQELSDYDWRSQEAREKYEQIKDLLGREMLDQ
                     RFAGMKQALENATDEDRQRVNEMLDDLNDLLDKHSRGEDTQQDFDDFMAKHGEFFPEN
                     PQNVEELLDSLAQRAAAAQRFRNSLSAEQRAELDALAQQAFGSPSLMNALNRLDGHLQ
                     AARPGEDWTGSSRFSGDNPMGMGEGTQALADIAELEQLAEALSQSYAGATMDDVDLDM
                     LARQLGEDAAVDAKTLAELERALVNQGFLDRGSDGQWRLSPKAMRQLGQTALRDVAEQ
                     LSGRHGERDTRRAGAAGELTGATRPWQFGDTEPWNVTRTLTNAVLRQAGTGVAELPMR
                     ITVDDVEISETETRTQAAVALLVDTSFSMVMENRWLPMKRTALALNHLVSTRFRSDAL
                     QIIAFGRYARTVSAAELTALEGVYEQGTNLHHALALAGRHLRRHPNAQPVVLVVTDGE
                     PTAHLEDFDGEGTSVFFDYPPHPRTIAHTVKGFDDVARLGAQVTIFRLGSDPGLARFI
                     DQVARRVEGRVVVPDLDGLGAAVVGDYLRSRRRR"
     gene            complement(3721..>4958)
                     /locus_tag="VFC01_24940"
     CDS             complement(3721..>4958)
                     /locus_tag="VFC01_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZN82897.1"
                     /translation="DDTVIPQLERALIAGHDVVLLGERGQGKTRLLRALPGLLDEWTP
                     VIAGAELGEHPYTPITPESIRRAAELGDELPVAWRHRSERYTEKLATPDTSVADLVGD
                     IDPIKVAEGRSLGDPETIAYGLIPRAHRGVVAINELPDLAERIQVSMLNVMEERDIQV
                     RGYTLRLPLDVVVVASANPEDYTNRGRIITPLKDRFGAEIRTHYPLELDAEVGVIKQE
                     AHLAAEVPEYLLQVLARFARHLRESHSIDQRSGVSARFAIAAAETVAAAATHRSAVLG
                     EQNPVARVCDLGTVIDVLRGKLEFESGEEGREQAVLEHLLRRATADTAQRLLGGIDVG
                     PLVTAIENGSAVTTGERVSARAVLDAVPEVPAIAQVQQRLGAKSDGERAAAVELSLEA
                     LYLAKRIDKVTGEGETVYG"
BASE COUNT          811 a         1685 c         1588 g          874 t
ORIGIN      
        1 cgtcggcgtc aagcgcaaga aacgccgccc ccccagtgtt tcggacagcg cctaaaccga
       61 aaatcgggcg gcctcgtaaa tgaggccgcc cgattcgttg aagcacctga ttaccctgtg
      121 ccgctggatg actcctcgcc ggcgctgatt ccaatctttt tgaggaacga tccatagcca
      181 cgcataccct caccgcccgg ggtaccgctc ccaaacagga tgggagagtc gccgatcgat
      241 ccgggagtga acttcaggga attcctggtg atgttttggg agtccaagcc actgttgtcg
      301 gcgacagtcg gagcgacctc tgttgcctcc actgccggct ccactggtgg tgcaggcgcg
      361 agttgatccg gaagagcggc gcttgccgcc tgcaccaccg acgggttgga atcggtggtc
      421 ggcgtggagg aggtcgatgc ggactcctga gcaagcgtcg ccttcgtcac cgcgacgttt
      481 aggatgtctg gcgcggcctc cggcggatcg ataccaaggt agtcgggcaa cgcacttaac
      541 ccattgatac cggccaacgc ggacggatca ctcagcgcct tcaagtaaac aggtaccgcg
      601 cccaaggtcg gcgcacccac aacggccagc aacgcgccca aggtcgtctc attcggatct
      661 gcgaggaagt cggtgtagag agtcgtcgcg ctcgtcgcct tcacgccatc aagcaacgcg
      721 cccaaggtcg tttcgctcgg atctgcgaga aacccggcgt aaagcggtac cgcgcccaag
      781 gtcggcgcac ccacaacggc cagcaacgcg cgcaaggtcg tctcattcgg atctgcgagg
      841 aagtcggtgt agagagtcgt cgcgctcgtc gccttcacgc catcaagcaa cgagcccaag
      901 gtcgtctcgc tcggatctgc caggaagtcg gcgtaaagcg gtaccgcgcc caaggtcgac
      961 gcacccacaa cggtcagcaa cgcgctcaag gtggtctcgc tcggatctgc gaggaagtcg
     1021 gtgtagagag tcgtcgcgct cgttccatac acgccatcaa gcaacgcgcc caaggtcgtc
     1081 gcgttcggat ttgccaggaa ggcttcgtac aagggcgatg cgctcaaact ggtcagcgca
     1141 gagagtttca cggatgccgt cttcgcgagc tgttctgcgg ccgggcccat cgtcggcagc
     1201 gttgctgccg ttgcgatgca cgccgcgcac gccatcgccg ttgctgaagt ggaaatcttc
     1261 gcccacacgg ttgtgacctc tctcggatgc ggagcctcga ccgcttgcca ggtgtatgac
     1321 attgagtgga cgagaacatc cgtccggtga gagcaatcta ccttaaaaaa ccctgacgca
     1381 cagcaaactt ccagcgagtt gggcaaaatt taactaaccg ccaaaatagc tcccggactc
     1441 acaaattagg tgacgcgcca gcccgacttg ggcgaacaac gtgggtcagc taagggcaca
     1501 gacataaaaa ggggcttgcg cgattccttc cgacggcgta tcccggcctg tctgtgacga
     1561 acccgcgccg cgatcgacac tcatcagggt tcgggagtgc taggcgattt gcttccgtca
     1621 accgagaagc gtgccgcgaa tcgtgtacgc cggagccctt catcgacgtt tgccaaactg
     1681 cctgaagccg atctcggccg ctacccgtct atgttcagag ctccccgcgc atgcgtcgcg
     1741 ctgtggcggg gttaccgccg cctgcgagac ctcagatagt cgccgaccac cgcggcgccc
     1801 aacccgtcca agtcgggcac cacgactctg ccctcgacgc ggcgggcgac ctgatctatg
     1861 aaacgggcca gacctgggtc gctgccgagc cggaaaatcg tcacctgcgc gcccagccgg
     1921 gcgacgtcgt cgaaaccctt gaccgtgtgg gcaatggtcc gcggatgcgg cgggtagtcg
     1981 aaaaacaccg acgtgccttc gccgtcgaag tcctcgaggt gcgcggtggg ctcaccgtcg
     2041 gtgacgacga gcaccaccgg ttgcgcgttg gggtgacgac gcaggtggcg tccggcgagc
     2101 gccaacgcgt gatgcaggtt ggtgccctgc tcgtagacgc cttccagcgc agtcagttcg
     2161 gcggcgctga ccgtgcgcgc atagcggcca aaggcgatga tttgcaaggc atccgagcgg
     2221 aaccgggtgc tgaccagatg gttgagggcc aacgcggtgc gcttcatcgg cagccagcgg
     2281 ttctccatca ccatcgagaa cgacgtgtcc accagcagcg ccacggcggc ctgcgtgcgc
     2341 gtctcggtct cggagatctc gacgtcgtcg acagtgatcc tcatgggcag ctccgccaca
     2401 ccggtaccgg cctgccgcag gaccgcgttg gtcagcgtgc gggtgacgtt ccacggttcg
     2461 gtgtcgccga actgccatgg ccgggtggcg ccggtcagct caccagcggc ccccgcgcgg
     2521 cgggtgtcgc gctcaccgtg acggcccgaa agttgttccg ccacatcacg caacgccgtt
     2581 tggccgagct ggcgcatcgc ctttggcgac agccgccatt gtccgtcgga gccgcggtcc
     2641 aggaaaccct ggttgaccag tgcccgctcc agctcggcga gcgtcttggc gtcgacggct
     2701 gcgtcctcgc ccagttggcg ggccagcatg tcgaggtcga cgtcgtccat ggtggcaccg
     2761 gcgtagctct gcgatagcgc ctccgcgagc tgttcgagct cggcgatgtc ggccagcgct
     2821 tgcgtgcctt cgcccatccc catcgggttg tcacccgaga accgcgatga gcccgtccag
     2881 tcctcaccgg gacgggccgc ctgcaggtgg ccatccagcc ggttgagcgc gttcatcagt
     2941 gacggcgagc caaaagcctg ctgcgccaac gcatccagct cggcgcgctg ttcggccgac
     3001 aggctgttgc ggaaccgctg ggcagccgct gcccgttggg ctaacgagtc gagcagctct
     3061 tcgacgttct gcgggttctc cgggaagaac tcgccgtgct tggccatgaa gtcatcgaag
     3121 tcctgctggg tgtcctcgcc acgagaatgc ttgtccaaca agtcattcag gtcgtcgagc
     3181 atctcgttga cgcgctgccg gtcctcgtcg gtcgcgttct ccagcgcctg cttcatgccg
     3241 gcgaaccgct gatcgagcat ctcgcggccc agcagatcct tgatctgctc gtacttttcg
     3301 cgcgcctcct ggctgcgcca gtcgtagtcg gacagctcct gcacggcctt ggccggcgac
     3361 gccggcagcg actcgatccg catctcgttg aaccgcgcgt cgtcgtcgag cgcgcgcgcc
     3421 agctccttgc gctcggccag cacggcctcg tcgagcagct tcttgatctc ctgcagcgtc
     3481 ccgtcaaggt tgttgcgttg caacagttcc cggcgccgac ggttcgcctc ggcggccagc
     3541 cggtcggcac cgcgcatatt cctggtgccg cgccgcatca gctcggagag cgcccgtcgc
     3601 ggcgagctgc cctccatcac gtcctggccg atctgctcca gcgcctcgcg caggtcgacg
     3661 ggcggcgcca gcgggtccgg cccgccggtg taggcggagt accgcgatga tcgcgcgtgt
     3721 ctagccatag acggtttcgc cttctcccgt caccttgtcg atgcgcttcg ccaaatacag
     3781 tgcctccagc gacagttcga ccgcggccgc gcgctcgccg tcggacttag cgccgaggcg
     3841 ctgctgaacc tgggcgatcg cgggcacttc cggcaccgcg tccaacaccg ccctcgcgga
     3901 tacccgctca ccggtcgtga cggccgagcc gttctcgatc gcggtcacca gcggcccgac
     3961 gtcgatgccg ccgagcagcc gctgtgcggt gtccgcggtg gcccgtcgca gcagatgctc
     4021 cagcaccgcc tgttcgcggc cttcctcgcc ggattcgaat tccagcttgc cgcgcagcac
     4081 gtcgatcact gtgccgaggt cgcacacccg ggccaccgga ttctgctcgc cgagcactgc
     4141 ggagcggtgc gtggcggcag cggcgacggt ctccgcggca gcgatcgcga accgcgccga
     4201 gacgcccgag cgctgatcga tcgagtgcga ctcacgcagg tggcgggcga accgcgcgag
     4261 cacctgcagc aggtactccg gcacctctgc ggccaggtgc gcctcctgtt tgatgacacc
     4321 gacttccgcg tcgagttcca gcgggtagtg ggtgcggatc tcggcgccga accggtcctt
     4381 gagcggagtg atgatgcggc cgcggttggt gtagtcctcg gggttcgcgc tggcgacgac
     4441 gaccacgtca agcggtagcc gcagcgtgta gccgcgcacc tgaatgtcgc gctcctccat
     4501 cacgttgagc atcgacacct gaatgcgctc ggccaggtcg ggcagctcgt tgatggcgac
     4561 gacgccccgg tgcgcacgcg gaatcagccc gtacgcgatg gtctccgggt cgccgaggct
     4621 gcggccctcg gccaccttga tcggatcgat gtcaccgacc aggtcggcca cgctggtgtc
     4681 cggggtggcc agtttctccg tgtaccgctc gctgcggtgc cgccacgcca cgggtagctc
     4741 gtcgcccagc tcggcggccc gccggatcga ctccggcgtg atcggggtgt acggatgctc
     4801 gcccagttcc gcgcccgcga tcaccggtgt ccactcgtcg agcagcccgg gcagcgcacg
     4861 cagaagccgc gtcttgccct gcccgcgttc gccgagcagc accacgtcgt gcccggcgat
     4921 cagggcccgc tccagctgcg ggatcacggt gtcgtcga
//
LOCUS       DASRJA010000567         6940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_507260,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000567 DASRJA010000000
VERSION     DASRJA010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507260"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VFC01_24945"
     CDS             <1..981
                     /locus_tag="VFC01_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HZN82898.1"
                     /translation="EGFIVSTQVASGQVLRIDPRNGSQTVLAQLTPGLDNLTFVGDRL
                     FVSNFTGEITQILPGGDTSTVLPGGLNWPLDLAVANDGNLYVADGTYFYLVRADGSLE
                     TAGMLFSPGYPGFLRGLAATGSDEFVVTTSGGQVATYRPAVNETDYLATDFDQLYGVA
                     VRPGGTVVFAELGTGRVHSLREGNVETLASGLHDPVGVAFDHNGRPLVAESGAGRVAR
                     LSGSNAETVVDGLQRPQGIVVDDGQLYIVDAGAKEVISFDLNTGDRRTIASGLPVGPP
                     PGVEPKPLKGMPPFSGPQGSFAGITVGPDGSLYVSADGDGSVLALRRVDT"
     gene            978..1715
                     /locus_tag="VFC01_24950"
     CDS             978..1715
                     /locus_tag="VFC01_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN82899.1"
                     /translation="MTVTPAEHRYLQVARTLRKEIVDGVYPVGSQLPTEHELCERFAV
                     SRFTIREALRRLRDDNLVASRPRAGTLVVPRPATNSYAQDVMSINDLLAFATGAPFTI
                     ESNAMVTIGDNLAARTGLTVGSEWLAVRGYRQADGTDAPVCKTEYYINRAFAAVGRLL
                     QRHSGPIFPLIEDLFGVSVVEVHQQISAVVVTPELAALLRIETGSAALEMQRTYKTSD
                     GEIAQVTVNTHPSARYRHSMTMRRVKG"
     gene            1726..3282
                     /locus_tag="VFC01_24955"
     CDS             1726..3282
                     /locus_tag="VFC01_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN82900.1"
                     /translation="MRVDEDRAAEAYRRGLWVRSTLADTLRDAARSTPGRTVLVDGDI
                     RLDCQTLHERASALAHMLLARMPPGSVVSFMLPNWHEAAIIYLAATLAGMVVNPVLPS
                     LRDRELQFILSDADSRTIFIPADFGKHDYVAMLDRVTSDMASPPEVVVLRGDARSHTP
                     YASLFADGAATGLPVLNPDSVRMILYTSGTTGRPKGVLHTHNSIHALISQIGEYWRVD
                     YGDVFLVPSPIAHIGGSIYAFECPLLLGTTAVLMERWDPDTAVQLMVTERCTHMAGAT
                     PFLVGLLAAAEQAETRLPDLKVFICGGASVPPSLIRGAAAYFEHTAVSRVYGSTEVPV
                     TTVGALGDIEHAAETDGHAGMADIKLVHGEIRARGPQMLVGYLHAEDETEAFDDEGFF
                     RTGDLGRWEDDKSLVVTGRAKDIIIRNGENISPKEIEDILVSHVGIAEIAVVGVPDER
                     TGERACAVIVASDQPAPDLAGLRELLMHKGVAKFKVPEQVEIWEALPKNDAGKVLKHQ
                     IRAKLTKVDV"
     gene            3284..4057
                     /locus_tag="VFC01_24960"
     CDS             3284..4057
                     /locus_tag="VFC01_24960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN82901.1"
                     /translation="MQVAIVTGASSGIGFGCAVKLAEAGIAVLGTGRDDSRLADLEKA
                     INDGDRVATLAVDLTADDAPQRIVDLAVQRWGHIDFLVNNAGVGSPKPLHETDDESLD
                     YFLGLMLRAPFRLARDVIPHMQPGSAIINVTSTFAVVGGLRGGAYSAAKGGLTALTLH
                     IACQYGPQGIRCNAVAPGVTLTPMVATRLEDERFRKINTEMTPYPRLGEVNDIASTVA
                     FLCSDGASFINGQTIVVDGGWSSTKYLSDFALTSEWVPR"
     gene            4054..5526
                     /locus_tag="VFC01_24965"
     CDS             4054..5526
                     /locus_tag="VFC01_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN82902.1"
                     /translation="MNLFALLDQAADRFGDRGALYLGEQQRSTWSELRDRALRLATSI
                     SRLDPSGARIAVASENSPEIVELMFAIWAAERVFVPINYKLHPREMEQILDDAGVTQV
                     FASAKIAAGLAPLTQVPIDIIGSDAYESRFSATPSEPPKNTDPSTVAWLFYTSGTTGR
                     SKGAMLSHRNLMAMTVSHLADFDAPDENCSLIHGAPMSHGSGLYVPPYVLRGARQVVP
                     ASGAFDPDEFLNLCEQHPGCSAFLAPTMVQRLVQTGRSCPRNLKTVVYGGGPMYVDSL
                     KKALAAFGPIFVQLYGQGEAPMTITGLRRADHLDADDAILGSVGYARSGVDVAVLAAD
                     GSPAAIDEIGEIVCRGDVVMSGYWKNPDATAATLRDGWLHTGDMGSFDARGFLTLRDR
                     SKDVVISGGSNIYPREVEEALIEHRGVSEACVVGAPDAEWGEVVVAFIVGSASEGELD
                     AHLLERIARFKRPKRYEFVDEIPKNSYGKVLKRELRARLQ"
     gene            complement(5638..5901)
                     /locus_tag="VFC01_24970"
     CDS             complement(5638..5901)
                     /locus_tag="VFC01_24970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82903.1"
                     /translation="MTIKRRYLPPMLLAVAAAASIAAAPTALAGQTCTYLSADSSVCQ
                     TPGNVQVVTSPPPVQYAPQFPYFGGNLIILHQHESHHGHGGHR"
     gene            6087..6191
                     /locus_tag="VFC01_24975"
                     /pseudo
     CDS             6087..6191
                     /locus_tag="VFC01_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887124.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
     gene            6336..6767
                     /locus_tag="VFC01_24980"
     CDS             6336..6767
                     /locus_tag="VFC01_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN82904.1"
                     /translation="MTVLDSPIPRFHLAMPVDDLGAACHFYGEVLGLEQGRSADAWVD
                     WNLQGHQFVTHLAPRGTQAVHNPVDGHDVPVPHFGLILTIPEFQKLADRLCAANTEFV
                     IEPYVRFEGEIGEQWTMFLRDPAGNALEFKAFADDAQVFAA"
     gene            6767..>6940
                     /locus_tag="VFC01_24985"
     CDS             6767..>6940
                     /locus_tag="VFC01_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZN82905.1"
                     /translation="MRVVIAGGHGKIALILEQLLSQRGDSVAGFIRNPDHATDLQAAG
                     AEALVVDLENSSVD"
BASE COUNT         1198 a         2184 c         2316 g         1242 t
ORIGIN      
        1 gagggcttta tcgtgtcgac ccaggtggcc agcgggcagg tgctgcgcat cgatccacgc
       61 aacggctccc agaccgtgct ggcgcagctg acccccggcc tcgacaatct gacgttcgtc
      121 ggtgaccggt tgttcgtctc gaacttcacc ggcgagatca ctcagatcct gccgggcggt
      181 gacacgagca ccgtgctgcc gggcgggctg aactggcccc tcgatctggc cgtggccaac
      241 gacggcaatc tgtacgtcgc cgacggcacg tacttctacc tggtgcgcgc cgacggatcg
      301 ttggaaacgg ccggcatgct gttctcgccc ggctatccgg gcttcctgcg cggcctggcc
      361 gcgacggggt ccgacgagtt tgtggtgacg acgtccggcg gacaggtggc aacgtatcgg
      421 cccgccgtta acgaaaccga ttacctcgca accgacttcg accagctgta cggtgtggcg
      481 gtcagacctg ggggcacggt ggtctttgcc gaactgggca ccggtcgcgt gcactccctt
      541 cgagaaggca atgtcgagac actggcatcc ggcctgcacg atccagtcgg cgtggccttc
      601 gaccacaacg gcagacccct ggtcgcggag tccggtgcgg gccgagtggc gcggctgtcc
      661 gggtcgaacg cggaaaccgt ggtcgacgga ctgcaacgcc cccagggcat cgtcgtcgac
      721 gacgggcagc tctatatcgt cgatgccggc gccaaggaag tcatctcatt cgacctcaac
      781 acgggcgacc gccgcacgat cgcttccggc ctgccagtcg ggccgccgcc cggtgtggaa
      841 cccaagccgc tcaagggaat gccgccgttc tccggaccac aaggttcgtt cgcgggtatc
      901 actgtggggc cggacggcag cctctacgtg tcggcggacg gcgatggcag tgtgttggcg
      961 ctgcgccggg tggacacatg acagtcacgc cggccgagca ccgctacctg caggtcgcgc
     1021 gcaccctgcg taaggagatt gtcgacggcg tgtatccggt cggctcacag ctgccgaccg
     1081 aacatgaact gtgtgagcgg ttcgcggtga gccgcttcac gattcgtgag gccttgcgcc
     1141 ggttgcgtga cgacaacctc gttgcgtcac ggccccgggc gggcacgctc gtcgtcccac
     1201 gcccggcgac gaattcctat gcgcaggacg tgatgtcgat caacgacctg ctcgccttcg
     1261 ccactggcgc accgttcacc atcgagtcga acgcgatggt gacgatcggc gacaatctcg
     1321 ccgcccggac cggcctgacc gtcggttcgg aatggctggc ggtgcgcggc taccggcagg
     1381 ccgacggcac cgatgcaccg gtctgcaaga cggagtacta catcaaccgc gccttcgccg
     1441 cggtgggcag gctgctgcaa cggcactccg gtccgatctt cccgctcatc gaagacctct
     1501 tcggggtgag cgtggtcgag gtgcatcaac aaatctccgc ggtggtggta acgcccgaac
     1561 tggccgccct actcaggatc gaaacaggta gcgccgcact cgaaatgcaa cgcacgtaca
     1621 agacctcgga cggtgagatc gcccaggtca ccgtgaacac ccatccgtcg gcgcggtatc
     1681 gccattcgat gactatgcgc cgggtcaagg gttaggttcg ggtttgtgag ggtcgacgaa
     1741 gatcgcgcgg ccgaggctta ccgccgcggg ctgtgggttc gtagcaccct cgccgacaca
     1801 cttcgcgacg ccgcacgcag tacgcccggg cgaacggtct tggtcgacgg tgacattcgg
     1861 ctcgactgcc aaacgctgca tgagcgggcg agtgcgctgg cccacatgct gctggcgcgg
     1921 atgccaccgg gcagtgtggt gtcgttcatg ttgcccaact ggcatgaggc cgcgatcatc
     1981 tacctggccg cgacgctggc cggtatggtc gtcaaccccg tcctgccgtc gctgcgagat
     2041 cgcgaactgc agttcatcct gtccgatgcg gacagccgca cgatcttcat accggccgat
     2101 ttcggcaagc acgactacgt cgcgatgctt gaccgggtca cctccgacat ggcctcaccg
     2161 cccgaggtgg tcgtcctgcg gggtgacgcc agaagccaca cgccgtatgc gtcgctgttc
     2221 gccgacggcg cggcgacggg cctgccggtg ctgaatccgg attcggtgcg catgatcctc
     2281 tacacatcgg gcaccaccgg acgaccgaaa ggtgttctac acacccataa ttcgatccat
     2341 gcgctcatct cacagatcgg cgagtactgg cgggtcgatt acggcgacgt gttcttggtc
     2401 ccgtcaccca tcgcacacat cggtggatcc atctatgcct tcgaatgccc attgctgttg
     2461 ggcaccacgg cggttctgat ggaacggtgg gatccggaca ccgcagtcca actgatggtc
     2521 accgagcgct gcacacatat ggccggagcc accccgttcc tcgtcggctt gttggccgcc
     2581 gccgagcagg ccgagacgcg gttgccggat ctcaaggtgt tcatctgtgg tggtgcgtcg
     2641 gtgccaccgt cgctgatccg cggggcggca gcgtatttcg agcatacggc ggtaagccgg
     2701 gtctatgggt cgacggaagt cccggtcacc acggttgggg cattgggtga catcgagcac
     2761 gcggcagaaa ccgacggcca cgcgggcatg gccgacatca aactggtgca cggtgagatc
     2821 cgggcgcgcg gcccgcagat gctcgtcggg tacctccatg ccgaggatga gaccgaagcc
     2881 ttcgacgacg aggggttctt tcgcacgggc gacctcggcc gctgggaaga cgacaagtcc
     2941 ctggtggtca ccggccgggc caaggacatc atcatccgca acggggagaa catctcgccc
     3001 aaggagattg aggacattct tgtcagccac gtgggcatcg ccgagatcgc cgtcgtcggt
     3061 gttcccgacg aacgcaccgg ggaacgtgcc tgcgccgtta tcgtggcgtc cgatcagccc
     3121 gcgccagatc tcgcgggcct gcgcgagctg ctgatgcaca aaggagtggc caagttcaaa
     3181 gtgcctgagc aggtagagat ttgggaggcg ctgccgaaga acgatgcagg caaggtgctc
     3241 aaacatcaga tccgggcgaa gctgacgaag gtggatgtgt gagatgcagg tagcgatcgt
     3301 gacgggcgcg agcagcggta tcggcttcgg gtgcgccgtg aaactcgccg aagcgggcat
     3361 agcggtcctc ggcacgggcc gcgacgacag caggctggcc gatctggaga aggcaataaa
     3421 cgacggcgat cgcgtcgcaa cgcttgccgt cgacctgact gccgacgatg cgccacaacg
     3481 gattgttgac ctggcggtgc agcggtgggg acacatcgac ttcctggtca acaacgccgg
     3541 cgttggcagc cccaaacctt tgcatgaaac cgacgacgaa agcctggact atttcctggg
     3601 tttgatgctg cgggcaccgt tccggctggc gcgcgacgtg atcccgcaca tgcagccggg
     3661 ttcggcgatc atcaacgtga cgtcgacgtt cgccgtcgtc ggcggtctgc ggggtggcgc
     3721 ctattccgcg gccaaaggtg ggttgacggc gttgaccctg cacatcgcct gccagtacgg
     3781 gccccagggt atccggtgca acgcggtggc tccgggcgtc acgctgactc cgatggtggc
     3841 gacccgcctc gaagacgagc gcttccgcaa gatcaacacc gagatgacgc cgtacccacg
     3901 gctgggtgag gtgaacgaca tcgcgagcac cgtggcgttc ctgtgctccg acggcgccag
     3961 cttcatcaac ggccagacca tcgtcgtcga cggcggctgg agttcgacga agtacttgtc
     4021 ggacttcgcg ctcacgtccg agtgggttcc gcgttgaacc tgttcgcact gctcgaccag
     4081 gccgccgacc gtttcggcga ccgcggcgcg ctgtatcttg gtgagcagca acgcagtacg
     4141 tggagcgaac tgcgggatcg ggcgttgcgg ctggccacct cgatcagcag gctcgacccg
     4201 tcgggcgcgc gcatcgcggt ggctagcgag aacagtccgg agatcgtcga gctgatgttc
     4261 gcgatttggg cggccgagcg ggtgttcgtc ccgatcaact acaagttgca tccacgtgag
     4321 atggagcaga tcctcgacga cgccggtgtg acgcaggtgt tcgcgtcggc gaagatcgcc
     4381 gcgggtctcg cgccgttaac ccaagtaccg atcgacataa tcgggtcgga cgcctacgaa
     4441 agccgtttca gcgcaacgcc gtcggaaccg ccgaagaaca ccgacccgtc gacggtggcg
     4501 tggctgttct acaccagcgg caccacgggc cggtcgaagg gtgcgatgct gtcgcaccgc
     4561 aacttgatgg cgatgacagt ctcgcatctc gcggacttcg atgcgcccga cgagaactgc
     4621 agcctgattc acggcgcgcc gatgtcgcac ggttcgggtt tgtacgtccc gccgtatgtg
     4681 ctgcgcggtg cgcggcaggt ggtgccggcg tcgggcgcgt tcgaccccga cgagttcctc
     4741 aacctctgcg aacagcatcc gggctgcagt gcattcctcg cgccgacgat ggtgcagcgc
     4801 ctggtacaga ccggccggtc gtgcccgcgc aatctgaaaa cggttgtgta cggcggcggt
     4861 ccgatgtatg tcgacagcct gaagaaggcg ttggcggcgt tcggcccgat cttcgtgcag
     4921 ctctacggtc agggcgaggc gccgatgacg atcacaggcc tgcgtcgagc cgaccatctc
     4981 gatgccgacg acgcgattct ggggtccgtt ggttatgcgc gatccggcgt cgatgtcgcg
     5041 gtgctggctg cggacggctc gccggcggcg atcgacgaga tcggtgaaat cgtctgccgc
     5101 ggcgatgttg tgatgtcggg ctattggaag aatcccgacg cgaccgccgc cacgctgcgc
     5161 gacggctggt tgcacaccgg cgatatgggg tccttcgacg ccagggggtt cctgacgctt
     5221 cgtgatcgct ccaaggatgt ggtgatcagc ggcggaagca acatctatcc gcgcgaggtg
     5281 gaagaggcgc tgatcgagca ccgtggggtg tcggaggcgt gcgtggtcgg cgcgcccgac
     5341 gcggagtggg gcgaggtcgt ggtcgcgttc atcgtcgggt cggcgtcgga gggcgaactc
     5401 gacgctcatc tgttggagcg catcgcccgg ttcaagcgcc cgaagcgcta tgagttcgtc
     5461 gacgagatac cgaagaacag ctacggcaag gtgctcaagc gggagttgcg cgcccgcctg
     5521 caatgacggc cgtagctggc ggcttggtcg cggagatcga gttgggcccg ctgcggtgca
     5581 gcgcgggggt cctctccgcg acgccattcg cgcgccatca cctgactgct cgccgggcta
     5641 gcgatgtccg ccgtggccgt ggtgagattc gtgttggtgg aggatgatca ggttgccgcc
     5701 gaagtacggg aactgcgggg cgtactgcac cgggggcggc gaggtaacga cctgcacatt
     5761 tccgggcgtc tggcacacgc tgctgtcggc gctgagataa gtgcatgtct gtccggccag
     5821 tgccgtgggt gcggctgcaa tggatgccgc ggcagccacc gccaacaaca tcggcggtag
     5881 gtagcgtcgc ttgattgtca tgattcactc cttgagcgtg aatacgccgt agatacggcc
     5941 gctcactcgc cgcgatggag ttgtcgggcc ccagtggggc gcttccgggg agaaactgcg
     6001 ctgagtttct aattggatca gcaccggcga gggcgccgat catcccaacc gacccgatcc
     6061 gcttgggcga agggcaatgg ccgcatcgat ggatgcgtcg gattgagacc agtccgttcg
     6121 tcaccacgca cgaatcgttg tgcggctgca tctttgacat ggcgaccggc aacctatacg
     6181 aggtcacgct cgcagctcca caggtagccg gcagcacgac cgaagtaacg gtcagttgtc
     6241 gtcctcgctg taggtacggc tgacggggtc accaaccgcc acgcagtttg ggaacctata
     6301 gtcgccgtct gcaaggtcgc ccgtagcatc agcacatgac cgttctcgac tccccgattc
     6361 cgcgctttca cctggcgatg cccgtggacg acctcggggc cgcctgccac ttctacggcg
     6421 aagttctcgg ccttgagcag ggccgcagcg ccgacgcttg ggtggattgg aaccttcagg
     6481 ggcaccagtt cgtcacccac ctcgcgccgc gcggcaccca ggctgttcac aatccggtcg
     6541 atggtcacga tgtccctgtg ccccacttcg gcctgatcct gaccatccct gaattccaga
     6601 agctggcaga ccggttatgc gcggccaaca ccgagttcgt catcgaaccg tatgtacgtt
     6661 tcgagggcga gatcggcgaa caatggacca tgttcctgcg cgacccagcc ggaaacgcct
     6721 tggaattcaa ggcgtttgcc gacgacgcac aggtgttcgc ggcctgatgc gcgtcgtcat
     6781 cgccggcggg cacggcaaga tcgccctgat cctggaacag ctgctctccc agcgcggcga
     6841 ctcggtcgcc ggcttcatcc gaaaccccga ccatgccaca gatcttcaag ccgccggcgc
     6901 ggaggcgttg gtcgtcgact tggagaactc atcagtcgac
//
LOCUS       DASRJA010000568         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2859405,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000568 DASRJA010000000
VERSION     DASRJA010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2859405"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFC01_24990"
     CDS             <1..319
                     /locus_tag="VFC01_24990"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HZN82906.1"
                     /translation="LASNEAYDPATGRWEQHAPMPTPRSGIAAAVLGGRMFVFGGEAP
                     AGTFNQAEAYDSRSNSWSSHARMPTARHGLGAAAVAGRIYVISGGPTPGASASAANEI
                     FVP"
     gene            397..771
                     /locus_tag="VFC01_24995"
     CDS             397..771
                     /locus_tag="VFC01_24995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN82907.1"
                     /translation="MTKVLYIEHDDDNLYMLKMRLERVGDFEVLAAEDSEKGCKMAVT
                     EHPDVILMDLEMPVADRWQPVRTLKNNPHTRDIPIIGMSAYALDSEREQAIAAGCDGF
                     DVKPIAFESLLATIRRVVANPK"
     gene            complement(947..1309)
                     /locus_tag="VFC01_25000"
     CDS             complement(947..1309)
                     /locus_tag="VFC01_25000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN82908.1"
                     /translation="MKILYVEDNDDNVYMLKNRLTRAGFTVIVATDGAQGIAMAVSEQ
                     PDLILMDLTLPNIDGEEATRRIKADPATQRIPVIALTANAMSGDREKALAAGCDDFDT
                     KPVEMPRLLEKIAALKPH"
     gene            complement(1275..>2664)
                     /locus_tag="VFC01_25005"
     CDS             complement(1275..>2664)
                     /locus_tag="VFC01_25005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN82909.1"
                     /translation="ALINDILDLSKIEAGRMDLHLESFPLAPHIEDVVKTIEPLAAKN
                     GNRIIVDCPAALGTIHADQTRFRQALLNLASNANKFTEKGTVTIAAQPQPLDGRDMIM
                     IAVTDTGIGMTEEQMGRLFQEFSQADTSTTRKYGGTGLGLAISRHFCRMMGGDITVES
                     TPGTGSTFTIRLPRIAQSTETLVTPARTEGRPEPAHPIAEEAEEPLILVVDDDATVRD
                     LVLRHLERGGFAAVAARGGQEGLRLVRELRPAAVTLDIMMPDLDGWTVLAAIKGDPEL
                     AGIPVVLMSIVDQKNRGYALGAADYLVKPVDRATLVETLRNICGATVGRVLLVDDDEM
                     VRRSVRQALEPIGWKVTEAENGQVAVDSITAARPDVIILDLMMPEMDGFEFMDELRGR
                     PDWKDVPVVVITAKDLTNEDRNRLNGGVERIIQKSDRDEMLRQLSREISKCVKRRTMG
                     SHEDSLRRGQRR"
BASE COUNT          459 a          853 c          824 g          528 t
ORIGIN      
        1 tttagcgtcg aacgaggcgt acgatcccgc aaccggtcgc tgggagcagc acgcgccgat
       61 gcccacgccg cgcagcggaa tcgcggcagc ggtgcttggt ggacggatgt tcgtgtttgg
      121 gggcgaggcc cctgccggca ccttcaacca ggccgaagcc tacgatagca ggagcaacag
      181 ctggagcagc cacgcgcgga tgccgacggc gcgacacggt ctcggtgcag cggcggtcgc
      241 gggcagaatt tacgtcatct cgggcgggcc gacgcccggt gcttcggcgt cggcagctaa
      301 tgagattttc gtgccgtgag gcattgtgag cacctgtcct gacgtgccgc ctcgactagg
      361 tgatcgaatg aagggctcgt tgaatagagg ctggtcgtga ccaaagtgct ctacatcgaa
      421 cacgacgacg acaacctcta tatgctcaag atgcgccttg agcgggtcgg cgatttcgaa
      481 gttctcgcgg ctgaggacag cgagaagggc tgcaagatgg ccgtgactga gcacccagac
      541 gtaattctga tggatctcga aatgccggtc gctgaccgat ggcaacccgt acgaaccctt
      601 aagaacaatc cgcacactcg cgacatcccg atcatcggca tgtctgcgta tgcgctggac
      661 agcgagcgag agcaggccat tgccgcgggt tgcgacgggt tcgatgtcaa accgatcgcg
      721 ttcgagtctc tgctcgccac cattcgtcgc gtcgttgcga acccgaaata ggtcacccgc
      781 tctgctggtc aggacggcgg gccgcccggc acgcgcacgg tgaacaccga gcctttaccc
      841 ggctcgctct ccgccgtgat gtcattgccc atcatctgac agaacctccg gctgatcacg
      901 agcccaaggc cggtgccgcc atacttgctt gcggtgacgc ggaatcctaa tgaggcttta
      961 acgctgcgat cttttcgagc aggcgaggca tctccaccgg cttggtgtcg aagtcgtcgc
     1021 aaccggccgc cagcgccttc tccctatcgc ccgacattgc attcgcagtg agggcgatga
     1081 ccgggatgcg ctgggtggcc gggtcggctt tgatccggcg ggtcgcctcc tcaccgtcga
     1141 tattggggag ggtcaggtcc atcagaatga ggtcgggctg ttctgacacc gccatggcaa
     1201 tgccctgcgc cccgtcagtc gcgacgatga cggtgaaacc ggcgcgcgtc agccggtttt
     1261 tcagcatgta gacgttatcg tcgttgtcct cgacgtagag aatcttcatg cgaccccatg
     1321 gtccggcgct tgacgcactt gctgatctcg cgactgagtt gacgcagcat ttcgtcgcgg
     1381 tcgctctttt gaatgatgcg ctcgacgccg ccattgaggc ggttgcggtc ctcgtttgtc
     1441 aggtccttcg cggttatgac cacgaccggg acgtccttcc aatccgggcg cccgcgcagt
     1501 tcgtccatga actcgaaccc gtccatctcc ggcatcatca ggtcgagaat gatcacgtca
     1561 ggacgcgcgg cggtgatcga atcgaccgcc acttggccgt tctcggcctc ggtcaccttc
     1621 catccgatcg gctccagcgc ctgccgcacg ctgcgacgca ccatttcgtc gtcatcgact
     1681 agaagcacgc gtcccacggt cgcgccgcag atgtttctca gtgtctccac cagcgtggca
     1741 cggtcgacgg gtttcaccag gtagtcggcc gccccaagtg cgtaaccgcg gttcttttgg
     1801 tcgacgatcg acatcagcac gaccggaatg cccgccagct cggggtcgcc cttgatcgca
     1861 gccagcacgg tccagccgtc gagatcgggc atcatgatgt cgagcgtcac cgccgccggc
     1921 cgcagctccc gcaccagccg aagcccttcc tggccgccgc gcgccgcgac tgccgcaaac
     1981 ccgccccgtt ccaggtgccg cagcacgaga tcgcgcacag ttgcatcgtc atcgaccacc
     2041 aggatcaatg gctcttccgc ttcctcggcg atcggatggg cgggctccgg gcgaccctct
     2101 gtgcgcgccg gagtcactaa tgtttcggtg ctctgcgcga tccgtggcag acggattgtg
     2161 aaagtcgagc ccgtccccgg tgtgctttcg acggtgatgt cgccgcccat catgcggcag
     2221 aaatgccggc taatggccag cccgaggccg gtaccgccgt atttgcgggt agtcgaggta
     2281 tccgcctgcg aaaattcctg gaacagccgg cccatctgct cttccgtcat accgattccg
     2341 gtatccgtga cggcgatcat gatcatgtcg cgtccatcga gcggttgtgg ttgcgcggca
     2401 atcgtcacgg tgcctttctc agtgaacttg ttggcgttgc tcgccaggtt gagcagcgcc
     2461 tgccggaacc gggtctggtc ggcgtggatg gtgccgagcg ccgccggaca atccacgatg
     2521 atccgattgc cgttcttcgc ggccagcggc tcgatggtct tgaccacgtc ctcgatatgc
     2581 ggcgccagcg ggaacgactc gagatgaagg tccatccgcc ccgcctcgat cttggacagg
     2641 tcgagaatgt cgttgatcag cgcg
//
LOCUS       DASRJA010000569        15184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4019302,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000569 DASRJA010000000
VERSION     DASRJA010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15184
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4019302"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFC01_25010"
     CDS             complement(<1..393)
                     /locus_tag="VFC01_25010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZN82910.1"
                     /translation="MAEQATSGAGPAQSDGRGSRGDREGRGRRDDRGGRGGRDDREKS
                     NYLERVVAINRVSKVVKGGRRFSFTALVIVGDGNGMVGVGYGKAKEVPAAIAKGVEEA
                     RKGFFRVPLIGSTITHPVQGEAAAGVVML"
     gene            complement(419..787)
                     /gene="rplR"
                     /locus_tag="VFC01_25015"
     CDS             complement(419..787)
                     /gene="rplR"
                     /locus_tag="VFC01_25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZN82911.1"
                     /translation="MGQNVSAARRVARLRRHARLRKKIEGTAETPRLVVNRSARHIHV
                     QLVNDLDGTTVAAASSIEADVRAVDGDKKAHSVRVGQLIAERAKAAGIEQVVFDRGGY
                     TYGGRIAALADAAREGGLKF"
     gene            complement(829..1368)
                     /gene="rplF"
                     /locus_tag="VFC01_25020"
     CDS             complement(829..1368)
                     /gene="rplF"
                     /locus_tag="VFC01_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZN82912.1"
                     /translation="MSRIGKQPVPVPSGVDVTIDGQNVSVKGPKGTLDLAVAEPIKVA
                     RDDDGAIVVTRPDDERRNRSLHGLSRTLVANLVTGVTEGYTIKMEIFGVGYRVVAKGS
                     NLEFALGYSHPVVINAPEGVTFAVETPTKFSISGIDKQKVGQIAANIRRLRKSDPYKG
                     KGIRYEGEQIRRKVGKTGK"
     gene            complement(1382..1780)
                     /gene="rpsH"
                     /locus_tag="VFC01_25025"
     CDS             complement(1382..1780)
                     /gene="rpsH"
                     /locus_tag="VFC01_25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003974254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZN82913.1"
                     /translation="MTMTDPIADFLTRLRNANSAYHDEVSLPHSKIKANIAEILKAEG
                     YISDYHTEDARVGKSLVVQLKYGPSRERSIAGLRRVSKPGLRVYAKSTNLPRVLGGLG
                     VAIISTSSGLLTDRQAARQGVGGEVLAYVW"
     gene            complement(1870..2055)
                     /locus_tag="VFC01_25030"
     CDS             complement(1870..2055)
                     /locus_tag="VFC01_25030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZN82914.1"
                     /translation="MAKKALVNKANKKPKFKVRAYTRCSKCGRPHAVYRKFGLCRICL
                     REMAHAGELPGVQKSSW"
     gene            complement(2060..2641)
                     /gene="rplE"
                     /locus_tag="VFC01_25035"
     CDS             complement(2060..2641)
                     /gene="rplE"
                     /locus_tag="VFC01_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZN82915.1"
                     /translation="MTTAEATKGAVKTPPRLKQRYREEIRDALFKEFGYANVMQIPGV
                     VKVVVNMGVGDAARDAKLINGAVNDLSLITGQKPEIRRARKSIAQFKLREGMPIGARV
                     TLRGDRMWEFLDRLISIALPRIRDFRGLSPKQFDGTGNYTFGLTEQSMFHEINVDDID
                     RPRGMDITVVTSATNDDEGRALLRALGFPFKEN"
     gene            complement(2643..2960)
                     /gene="rplX"
                     /locus_tag="VFC01_25040"
     CDS             complement(2643..2960)
                     /gene="rplX"
                     /locus_tag="VFC01_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZN82916.1"
                     /translation="MNVHKGDTVLVISGKDKGAKGKVLVAYPERNKVLVEGVNRIKKH
                     TPVSRTERGAASGGIVTQEAPINVSNVMLLDSDGKPTRVSFRVDDETGKKVRVAKTNG
                     KDI"
     gene            complement(2961..3329)
                     /gene="rplN"
                     /locus_tag="VFC01_25045"
     CDS             complement(2961..3329)
                     /gene="rplN"
                     /locus_tag="VFC01_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZN82917.1"
                     /translation="MIQQESRLKVADNTGAKEILCIRVLGGSSRRYAGIGDVIVATVK
                     DAIPGGNVKRGEVVKAVIVRTVKERRRADGSYIKFDENAAVIIKPDNDPRGTRIFGPV
                     GRELREKRFMKIVSLAPEVL"
     gene            3659..4591
                     /locus_tag="VFC01_25050"
     CDS             3659..4591
                     /locus_tag="VFC01_25050"
                     /inference="COORDINATES: protein motif:HMM:NF038012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN82918.1"
                     /translation="MVAVTALALAAAFLFALSAFLQQRAARTIVGADTASLRDASGVK
                     RLLGHLVRSRTWLAGWLTNLGGVGTQAAALKVGSVATVQPLMAAQLLFVLSLASGEQR
                     RWPSARDWLSALVVCAGLVILLTADASSLTGGPHRNRVFMATVCVVSLVVALRQVSRH
                     TFGWLAGLLVGVAAGLCHALNAVYLKLTVEDLYHGGALATLLDWPVYALAATAVSGML
                     LVQIAFASGPLPPAVAAMSVTNPVASFVLGILAFDAPAPRDPGVLSAIAVSGVLIAVG
                     IVGLANASSTRRLYLPDPLPIGAVAADHGCHAAD"
     gene            4578..5339
                     /locus_tag="VFC01_25055"
     CDS             4578..5339
                     /locus_tag="VFC01_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN82919.1"
                     /translation="MQLTDSVVLVTGASAGIGHAAAARFAAKGARVLVHGRDPERTQQ
                     VADEVRGRAVFADLASPAGRDRLAAEALDAFGRVDVLVNNAGFGYAGRFSEMGVDAIR
                     RLIEVDLLAAIELTRSLLPGMVERRRGAVCFVTSIAGRTGVAGEAVYSATKAGLDAFA
                     DSLRGETARSGVNISVVVPGVVDTGFFESRGSPYTRTRPRPIPADTVADAVVRAVERD
                     RAEEWRPRWLRIAPTVRALAPGTFRRLAIRFGGSA"
     gene            complement(5331..6212)
                     /locus_tag="VFC01_25060"
     CDS             complement(5331..6212)
                     /locus_tag="VFC01_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HZN82920.1"
                     /translation="MLTELVELPGGSFRMGSTQFYPEEAPIHTATVAAFAVERHPVTN
                     AQFAEFVADTGYVTVAEQPPDPALYPGAAPQDLVPGALVFRPTDGPVDLRDWRQWWAW
                     VPGADWRHPFGPDSGIEDRLDHPVVQVAYPDAAAYARWAGRRLPTEAEWEYAARGGAT
                     TTYAWGDDAVPGGQLMANTWQGRFPYQNDGALGWSGTSPVGTFPPNGYGLVDMIGNVW
                     EWTTTQFSAHHRLDGPIESCCGPSTPDPSVNQTLKGGSHLCAPEYCHRYRPAARSPQS
                     QDSATTHIGFRCAASSR"
     gene            complement(6218..6907)
                     /locus_tag="VFC01_25065"
     CDS             complement(6218..6907)
                     /locus_tag="VFC01_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82921.1"
                     /translation="MKRVGLAALALACLLAACTRTSDGVPVAGEGGPWTVAPSPPSTT
                     TGPTQQNESSFGIVPTTRAPVPPNTVACSQTIQPSVRMTAEVDDPQAPKITVGVPDKW
                     SMTAGTGDVGGKLNGPGGMEATVTIAATQLDPAAAFRKYADDLIAESAVSTVSVLPGE
                     LCGYSGQKLMGAWSDTPQNAVEYEDRIAHIWTNSGTNYLVAVHVQAPTGTPGFDAAAA
                     LLTEDFEILLP"
     gene            complement(6907..9258)
                     /locus_tag="VFC01_25070"
     CDS             complement(6907..9258)
                     /locus_tag="VFC01_25070"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZN82922.1"
                     /translation="MATEFNGKIELDIRDSEPDWGPYAAPTAPAGAPNVLYLVWDDTG
                     IATWDCFGGLVEMPTMSRIAERGVRLSQFHTTALCSPTRASLLTGRNPTTVGMATIEE
                     FTDGFPGCNGRIPDETALLSEVLVENGYNTYCVGKWHLTPLEESNLAATKRHWPLSRG
                     FERFYGFMGGETDQWYPELVYDNHPVAPPALPEDGYHLSKDIADKTIEFIRDAKVIAP
                     DKPWFSYVCPGAGHAPHHVFKEWVDKYAGKFDMGYERYREVVLENQKRLGIVPPDTEL
                     SPINPYLDVKGPNGEPWPPQDTVRPWESLGDDEKRLFARMAEVFAGFLSYTDAEIGRI
                     LDYLDESGQLDNTIIVVISDNGASGEGGPNGSVNEVKFFNGYIDTVEDSLRVIDNLGG
                     PETYNHYPIGWAMAFNTPYKLYKRYASHEGGIADTAIISWPKGIAAHGEVRDTYVNVC
                     DITPTVYDLLSITPPATVRGVPQKPLDGVSIKLALDDPAVPTGKATQFYAMLGTRGIW
                     DKGWFANTVHAASPAGWSNFDKDRWELYHIEADRSQCHDLAAEHPDKLEELKALWFSE
                     ADKYNGLPLADLNILETMSRYRPYLAGTRESYLYYPGTSDVGMGAAVEIQGRSFSIIA
                     EVTIDTTGAEGVIIKHGGAHGGHVMFLQDGRLHYVYNFLGEEEQQMASSDAVPLGKHT
                     FGVAYVRTGTVEGSHTPLGDAALYIDDAEVASRPGMTIHPGTFGLAGATLSVGKNTGS
                     PVSRAYKAPFPFTGGTIAQVNVDVSGKPYVDLEREFARAFAKD"
     gene            complement(9311..9997)
                     /locus_tag="VFC01_25075"
     CDS             complement(9311..9997)
                     /locus_tag="VFC01_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HZN82923.1"
                     /translation="MAYAPTTRGRRWPAPALAVVVVAFLAFSLPPYFTGGTRVPATFG
                     LHYPLLVAHVMFASVAILTAVAQIWPRLRARRPALHRRIGRVYVYAAVPAAVCAMVIG
                     AATPFGPFLAVSNVLLASLWLWFTINGYVAARQRRFAQHRRHMVRSATLALSIITNRI
                     WTPVLIITCQPLQDSVFGGNEEHFVWFVAGVGAWLGWTIPFFTVQWWLNRRHVLPPSS
                     ISQLTATRRV"
     gene            10054..10758
                     /locus_tag="VFC01_25080"
     CDS             10054..10758
                     /locus_tag="VFC01_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN82924.1"
                     /translation="MTTDEPLPRALEILWRDGPATRRTRGLNRERIVAAAIELADSDG
                     LAALSMGRLAERLKCGTMSLYRHVANKDELLMFMLSTAPDPPPNTNRTNWRGALTDWA
                     CGLWDVYHRHPWVLQATAAAGPPADPGQLAWLDAGLAALEATELAERDKLAAVMAVLH
                     FVRGAVALDIEGAPDDAPEYPALLRRVLDPARFPALAAAVEAGVFDDANDDHLAEFRS
                     GLDQLLDGIDAKVGRR"
     gene            10795..11115
                     /locus_tag="VFC01_25085"
     CDS             10795..11115
                     /locus_tag="VFC01_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005074218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HZN82925.1"
                     /translation="MDTGPITLINVFEIDSRDVELFLREWQERAQFMGRQPGFRSLRL
                     HRALNPDSRFQLINVAEWDSPEALQTATAQGFFQQSAQRSVQDFAVTAHPAVYRVVIE
                     ATAE"
     gene            11169..11606
                     /locus_tag="VFC01_25090"
     CDS             11169..11606
                     /locus_tag="VFC01_25090"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HZN82926.1"
                     /translation="MRSLIRAGARIGTFAVLTVPGRRTGRPVHVPLVVFSDGGNRYLV
                     ASYGIVNWVRNLRAADGRAELRRGHTTEKITTVELPPEQAGPILRISLQSGPPGIPRP
                     IVRIFRRFFVLPYLDVDLNSSVDDFVRSAVNHPVFRVQPGVQS"
     gene            11692..13200
                     /gene="zwf"
                     /locus_tag="VFC01_25095"
     CDS             11692..13200
                     /gene="zwf"
                     /locus_tag="VFC01_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZN82927.1"
                     /translation="MTISKPQTISYPAPGARPYRQDDIAVDPHVVVLFGATGDLARRK
                     LIPGLAYLDQSELAPHIEIVATSLEDISTEEFLEVAKAAIDDFGTHKLTKEQWSRFAE
                     TVTYVPQSAGPEALATAVANAETKLGSGARRLHYLSVPPKAARAVIEMLRDADLVDHS
                     RVVMEKPFGTDLASAVALNDFVHETFREDQIFRIDHFLGKEAALNILAFRFANGLFEP
                     IWNRNFIDHIQIDIPETLGLDERANFYESTGAYKDMVVTHLFQVMAFVVMEPPTALEP
                     RAISEEKNKVFRSMLPVDPDNVVRGQFTGYRELDGVARDSDTETFIALKVGIDNWRWA
                     GVPVYLRTGKRMAEGMRIISIAFKEAPRTMFAPGSGVGAHGPDHLTFDLADNSKVSLS
                     FYGKRPGPGMKLDKLSMQFSTQEVDTVSDVLEAYERLILDAMRGDHTLFTTAEGIESL
                     WERSADLLADPPPAKAYPQGTWGPNAIHQLIAPNGWRLPFERAWREAKNNDS"
     gene            complement(13262..13558)
                     /gene="rpsQ"
                     /locus_tag="VFC01_25100"
     CDS             complement(13262..13558)
                     /gene="rpsQ"
                     /locus_tag="VFC01_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZN82928.1"
                     /translation="MAETKGPKRTPRQEKPRGRRKTRIGYVVSDKMQKTIVVELEDRK
                     SHPLYGKIIRTTTKVKAHDEDSIAGIGDRVSLMETRPLSATKRWRLVEILEKAK"
     gene            complement(13558..13791)
                     /gene="rpmC"
                     /locus_tag="VFC01_25105"
     CDS             complement(13558..13791)
                     /gene="rpmC"
                     /locus_tag="VFC01_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZN82929.1"
                     /translation="MAVGVSPGELRELTDDELKDRLRESKEELFNLRFQMATGQLANN
                     RRLRTVRQEIARVYTVLRERELGLASGPAGEES"
     gene            complement(13791..14207)
                     /gene="rplP"
                     /locus_tag="VFC01_25110"
     CDS             complement(13791..14207)
                     /gene="rplP"
                     /locus_tag="VFC01_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZN82930.1"
                     /translation="MLIPRKVKHRKQHHPRQRGVASGGTSVSFGDYGIQALEHAYITN
                     RQIESARIAINRHIKRGGKVWINIFPDRPLTKKPAETRMGSGKGSPEWWIANVKPGRV
                     LFELSYPDEKTAREALTRAIHKLPIKARIVTREEHF"
     gene            complement(14211..15068)
                     /gene="rpsC"
                     /locus_tag="VFC01_25115"
     CDS             complement(14211..15068)
                     /gene="rpsC"
                     /locus_tag="VFC01_25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZN82931.1"
                     /translation="MGQKINPHGFRLGITTDWKSRWYADKQYKDYVKEDVAIRRLLAT
                     GLERAGIADVEIERTRDRVRVDIHTARPGIVIGRRGTEADRIRADLEKLTGKQVQLNI
                     LEVKNPESQAQLVAQGVAEQLSNRVAFRRAMRKAIQSAMRQPNVKGIRVQCSGRLGGA
                     EMSRSEFYREGRVPLHTLRADIDYGLYEAKTTFGRIGVKVWIYKGDIVGGKREATATV
                     PSGADRPRRERPSGTRPRRSGASGTTATSTEAGRAATGTEDAPAVAEATTGTEAAAAE
                     ATTTENTES"
BASE COUNT         2411 a         5043 c         4916 g         2814 t
ORIGIN      
        1 cagcatcacc acacctgcgg cggcctcacc ctgcaccggg tgggtgatgg tcgagccgat
       61 cagcggaaca cggaagaagc ccttgcgagc ctcctcgaca cccttggcga tggcggcggg
      121 aacttccttg gccttgccgt agccgacacc gaccatgccg ttgccgtcgc cgacgatcac
      181 caacgcggtg aagctgaacc gccgaccacc cttgacgacc ttggagacgc ggttgatcgc
      241 gacgacgcgc tcgaggtagt tgctcttctc acggtcgtcg cggccaccac ggccaccgcg
      301 gtcgtcgcgg cggccacggc cctcgcggtc accgcgcgaa cctcggccgt cggattgcgc
      361 cggcccggca cccgatgttg cctgctcggc catcatgcag tccttccgtt gtacgtcatt
      421 agaacttcag cccgccttcg cgcgccgcgt ctgccagcgc cgcgatgcgg ccaccgtagg
      481 tgtagccacc gcggtcgaac acgacctgct cgattccggc ggccttggcg cgctcggcga
      541 tcagctgtcc gacccgcacg ctgtgggcct tcttgtcacc gtcgaccgcg cgcacgtcag
      601 cctcgatcga cgacgcggcc gccacggtgg tgccgtcgag gtcattgacc agctgcacat
      661 gaatgtgccg cgctgaacgg ttgaccacca ggcgcggcgt ctcggccgtg ccctcgatct
      721 tcttgcgcag ccgggcgtgc cgacgcagtc gtgcgacccg ccgtgccgca gaaacgttct
      781 gtcccaccgg cttacgcgcc gcagactctg ttgtcttcgc agccatatct acttacctgt
      841 ctttccgacc ttgcggcgga tctgctcgcc ctcgtagcgg atgcccttgc ccttgtaggg
      901 gtcgctcttc cgcaggcggc ggatgttggc cgcgatctgg ccgaccttct gcttgtcgat
      961 gccggagatc gagaacttcg tcggggtctc caccgcgaag gtgacgccct ccggggcgtt
     1021 gatgacgacg ggatggctgt agccgagcgc gaactccagg ttgctgccct tggccaccac
     1081 gcggtagccg acgccgaaga tctccatttt gatggtgtag ccctcggtca caccggtgac
     1141 gaggttggcc accagcgtgc gcgacagccc gtgcagcgaa cggttgcgcc gctcgtcgtc
     1201 ggggcgggtc accacgatgg cgccgtcgtc atcgcgggcg accttgatcg gctcggcgac
     1261 cgccagatcc aaagtgccct tcggtccctt gaccgacacg ttctggccgt cgatcgtcac
     1321 gtcgaccccg gacggaaccg ggaccggctg ctttccaata cgcgacattt ctagctctcc
     1381 ctcaccacac gtaagcgagg acttcgccgc ccacgccctg tcgtgctgcc tgacggtcgg
     1441 tgagcagacc cgacgacgtg gagatgatcg ccacgccaag gccacccaga acccggggca
     1501 gattggtgga tttcgcatag acgcgcaggc cgggcttcga cacccggcgc agcccggcga
     1561 tgctgcgctc acggctgggg ccgtacttca gttgcaccac cagcgacttg cccacgcggg
     1621 catcctcggt gtggtagtcg gagatgtagc cctccgcctt gaggatctcg gcgatgttgg
     1681 ccttgatctt gctgtgcggc agggacacct cgtcgtggta cgccgaattg gcgttgcgca
     1741 gacgtgtcaa gaagtctgcg atcgggtccg tcatggtcat gacagccggt tcaccttcct
     1801 cgcggcggtt cccactgtgc ccaattcttg gggaggccta ccgcaacctt tgttgatgta
     1861 gatgtgctgt taccagctgg acttctgcac gcccggcagc tcgccggcat gcgccatctc
     1921 acgcagacag atgcggcaga gcccgaactt gcggtaaacc gcatgcgggc gaccgcactt
     1981 gctgcagcgg gtgtatgccc gcaccttgaa cttgggcttc ttattggcct tgttgaccag
     2041 agccttcttt gccatctgtt cagttctcct tgaaggggaa gccgagggcc cgcaacagcg
     2101 cccgtccttc gtcgtcgttc gtcgccgagg tgacgacggt gatgtccatg ccacgggggc
     2161 ggtcgatgtc gtcgacgttg atctcgtgga acatcgactg ctcggtcagc ccgaaggtgt
     2221 agttgccggt gccgtcgaac tgcttgggcg acaggccgcg gaagtcgcgg atacgcggca
     2281 gtgcgatcga gatcagccgg tcgaggaatt cccacatccg gtcgccgcgc agggtgaccc
     2341 gggcgccgat cggcatgccc tcgcgcagct tgaactgcgc gatggacttg cgggcccgcc
     2401 ggatttccgg cttctgcccg gtgatcagcg acaggtcgtt gaccgcgccg ttgatcaact
     2461 tggcatcgcg ggcggcgtcg ccgacaccca tgttcacgac gaccttcacc acgccgggga
     2521 tctgcatgac gttggcgtag ccgaactcct tgaacagcgc atcgcggatc tcttcgcggt
     2581 agcgctgctt caaacgcgga ggagtcttca ctgcgccttt tgtggcttct gcggtcgtca
     2641 tctcagatgt ccttgccatt ggtcttcgcg acgcggacct tcttgccggt ctcatcgtcg
     2701 acgcggaagc tcacgcgggt cggcttgccg tcggagtcga gcagcatcac gttggacacg
     2761 ttgatcggcg cctcttgcgt gacgatgccg ccggaggccg ctccgcgctc ggtccgggaa
     2821 acaggggtgt gcttcttgat ccggttaacg ccctcgacga gcaccttgtt gcgctcgggg
     2881 taggccacca gaaccttgcc cttggcgccc ttgtccttgc cggaaatcac gagcaccgtg
     2941 tcgcccttgt ggacgttcat ctacaacacc tccggggcga gcgagacgat cttcatgaag
     3001 cgcttctcac gcagctcgcg gccgaccggg ccgaagatgc gcgtgccacg cggatcgttg
     3061 tcgggcttga tgatgacggc ggcgttctcg tcgaacttga tgtagctgcc gtcggcgcgg
     3121 cggcgctcct tgacggtgcg cacgataacg gccttgacca cttcaccgcg cttgacgttg
     3181 ccgccgggga tggcgtcctt gacggtcgcc acgatgacgt cgccgatgcc ggcgtagcgc
     3241 cgcgacgagc cgccgagcac gcggatgcac aagatctcct tggcgcccgt gttgtcggcg
     3301 accttcagcc gcgattcctg ctgaatcaca gtctctcctc gacctggtta atgtgtgcac
     3361 ggcagatgcc gacctgacgt gcgcggtctc ttgctgtttc cgaagggcac gcagggtttt
     3421 ctttcgagaa ccgaggtctg cccaaggcaa ccgtttgatt ctaggtgacg acctgcgcgg
     3481 aaccaaatcg ccgctggtca cgctctctca ccccgcgaac gtgggttacc ggcacgcatt
     3541 ttgcccctca aacgtgcatc aagcccacac tcggcgcaca acgtgagagt cacatcgtca
     3601 aaacctgaca ttccgcgcac gttcacctcg gcgtattacg cccggtcaaa ctggcagcat
     3661 ggtcgccgtc accgcgctgg ccctggcggc cgcgttcctc ttcgcgttgt cggcgttcct
     3721 ccagcagcgc gccgcgcgca cgatcgtcgg cgcagacacc gcatctctgc gagacgcttc
     3781 gggagtcaag cggctgttgg gccatctcgt gcgaagcagg acgtggctgg ccggttggct
     3841 gacgaatctc ggcggggtcg gcacacaggc ggcggcgctc aaggtcgggt cggtggccac
     3901 ggtccagccg ctgatggcgg cacagctgct gttcgtgctg tcactggcct cgggcgagca
     3961 gcgccgctgg ccctcggcgc gtgactggtt gtcggcgctg gtggtctgcg ccggtctggt
     4021 gatcttgctg accgccgacg catcgtcgct caccggcggg ccccatcgga accgcgtatt
     4081 catggcgacg gtgtgtgtcg tcagcctggt ggtcgcgctg cgccaggtga gtaggcacac
     4141 gttcggatgg ctggccggcc tgctcgtcgg tgtggccgcc ggactatgcc acgcgctcaa
     4201 cgccgtttac ctcaagctga cggttgagga cctgtaccac ggcggagcgt tggccacgct
     4261 gctcgattgg cccgtctacg cgttggccgc gaccgccgtg agcggaatgt tgttggtaca
     4321 gatagccttt gccagcggtc cgctcccgcc tgccgtcgca gccatgagcg tcaccaaccc
     4381 cgtcgccagc ttcgtcttgg gaatcctcgc cttcgatgcg cccgctcccc gcgaccccgg
     4441 cgtgctgtcg gccatcgcgg tatccggggt gctgatcgcg gtcggcatcg tgggcctggc
     4501 gaacgcgtcg agcacccgcc ggctgtactt gcccgatccg ctcccaatcg gcgcggtcgc
     4561 cgctgatcat ggttgccatg cagctgactg actcggtcgt gcttgtcact ggcgcgtcgg
     4621 ccggaatcgg gcatgccgcc gctgcgcggt tcgccgcgaa gggcgcacgg gtgttggtgc
     4681 acggccgcga tcccgaacgc acacaacagg tggccgacga ggtgcgcggg cgggccgtgt
     4741 tcgcggatct ggcctcaccg gcgggccggg atcgactcgc cgctgaggct ctggatgcgt
     4801 tcgggcgggt cgacgtgctg gtgaacaatg ccggattcgg ctatgccgga cggttctcgg
     4861 aaatgggtgt cgacgcgatc aggcgcctca tcgaggtcga tctgttggcc gcgatagagc
     4921 tgacccgctc gctgctgccg ggcatggtcg aaaggcgccg cggcgcagtc tgtttcgtga
     4981 cgtcgatcgc cgggcgcacc ggtgtcgccg gtgaagccgt gtactcggcc accaaagcag
     5041 gtctcgatgc cttcgcggac agcctgcggg gcgagaccgc ccgatccggt gtgaacatca
     5101 gcgtcgtcgt tcccggcgtc gtcgacaccg ggttcttcga gtcgcgcggc agcccctata
     5161 cccgcacgcg gccacgaccg atacccgccg acacggtcgc cgacgccgtc gtccgcgccg
     5221 tggagcgcga ccgcgccgag gagtggcggc cgcggtggct gcgcatcgcc ccgaccgtgc
     5281 gcgcgctcgc tcccggcacc ttccgacgtc tggctatccg cttcggcgga tcagcgtgaa
     5341 ctggccgcgc agcggaaccc gatatgcgtc gtcgcgctgt cctgcgactg cggcgagcgg
     5401 gcggcgggcc ggtagcggtg gcagtactcc ggcgcgcaca ggtgcgagcc gcccttgagc
     5461 gtctgattca ccgacgggtc cggtgttgac ggcccacaac aggactcgat aggtccgtct
     5521 agtcggtggt gcgccgagaa ttgcgtcgtc gtccactccc acacattgcc gatcatgtcg
     5581 accaggccgt acccgttcgg cggaaacgtg ccgaccggcg atgtgcccga ccagcccaga
     5641 gcgccgtcgt tctggtaggg gaaccgtccc tgccaggtgt tggccatcaa ctgaccgccg
     5701 ggcacagcgt cgtcacccca tgcatacgtc gtcgtggcgc caccgcgcgc cgcgtactcc
     5761 cactcggcct cggtcggcag ccgtcgacca gcccaacgcg catacgccgc agcatccggg
     5821 taggccacct gcaccaccgg gtggtcgagt cggtcttcga tgccgctgtc aggaccgaac
     5881 gggtgacgcc agtcggcgcc cggcacccag gcccaccact gccgccaatc acgcagatcc
     5941 accgggccgt cggtgggccg gaacaccagc gcacctggca ccagatcctg cggcgcggct
     6001 cccgggtaca gcgcaggatc cgggggctgc tcagcgacgg tgacgtaacc ggtgtcggcg
     6061 acgaactcgg cgaactgggc gttggtcacc ggatggcgtt cgacggcgaa agcggcgacg
     6121 gtcgcggtgt gaatcggcgc ctcttccgga tagaactgcg ttgagcccat ccggaaggac
     6181 ccacccggca gctcgaccag ctcggtgagc atgacactca cggtaggagg atttcgaagt
     6241 cctcggtcag cagcgccgcg gccgcatcga agccgggggt tcccgtcggg gcttgcacgt
     6301 gcacggcgac gagatagttg gtgccgctgt tggtccagat atgggcgatg cggtcttcgt
     6361 actcgacggc attctgaggc gtatccgacc acgcacccat caacttctgc ccgctgtagc
     6421 cgcacagttc gccgggtagc acgctgacgg tgctgacggc cgattccgct atcagatcgt
     6481 cggcgtactt cctgaacgcg gcggccggat ccagctgggt cgcggcgatc gtgaccgtcg
     6541 cctccatacc gcccggaccg ttcagcttgc cgccgacatc accggtgccc gccgtcatcg
     6601 accatttgtc agggacgccg accgtgatct tgggcgcttg cgggtcgtcg acctccgccg
     6661 tcatccgcac gctgggttgg atcgtctgag aacacgcgac ggtgttcggc ggcacggggg
     6721 cccgcgtcgt gggaacgatg ccgaacgagg actcgttctg ctgtgtcggc ccggttgttg
     6781 tcgacggcgg cgacggggcg actgtccagg ggccgccctc gccggcgaca ggcaccccgt
     6841 cgctggtgcg ggtgcacgca gccagcaggc aggccagcgc gagcgccgcc aatccaacgc
     6901 gcttcatcag tccttcgcga aagcccgcgc gaactctcgt tccaaatcca cgtacggctt
     6961 gcctgacacg tcaacgttca cctgtgcgat ggtgccacct gtgaacggga acggcgcctt
     7021 gtaggcccgc gacaccggcg atccggtgtt cttgccgaca ctcagggtgg ccccggccag
     7081 gccaaaggtt cccggatgga tcgtcatgcc cggccgcgac gccacctcgg catcgtcgat
     7141 gtagagcgcg gcgtcgccga gcggggtgtg gctaccctcc accgtcccgg tacgcacgta
     7201 ggccacgccg aaggtgtgct tgcccagcgg gacggcatcc gacgacgcca tctgctgctc
     7261 ttcctcgccg aggaagttgt agacgtagtg cagtcgtccg tcctggagga acatcacatg
     7321 gccgccgtgc gcgccaccgt gtttgatgat gacgccctcg gcgcccgtgg tgtcgatggt
     7381 cacctcggcg atgatcgaga acgaccgacc ctggatctcg acggccgcgc ccatgccgac
     7441 atcagaggtg ccggggtagt acaagtacga ctcgcgggtg cccgccaggt acggccgata
     7501 ccgcgacatc gtctccagaa tgttcaagtc cgccagcggc aatccgttgt acttgtcggc
     7561 ctcgctgaac cacagcgcct tgagttcctc gagcttgtcg ggatgctcgg cggccagatc
     7621 gtgacactgg ctgcggtcgg cctcgatgtg gtacagctcc cagcggtcct tgtcgaagtt
     7681 cgaccagccg gccggcgatg cggcgtgcac ggtgttggcg aaccagccct tgtcccagat
     7741 accgcgggtg cccagcatcg cgtagaactg cgtcgccttg ccggtgggta ccgccggatc
     7801 atcaagggcc agtttgatac tgacgccgtc gagtggtttc tgcggaacgc cccgcacggt
     7861 tgccggcggt gtgatggaga gcaagtcgta caccgtcggg gtgatgtcgc agacgttgac
     7921 gtaggtgtcc cgcacttccc cgtgcgccgc gattcccttg ggccaggaga tgattgcggt
     7981 gtcggcgatc ccgccttcgt gtgaggcgta ccgcttgtac aacttgtacg gcgtgttgaa
     8041 cgccatcgcc catccgattg ggtagtggtt gtaggtctcc ggcccgccga ggttgtcgat
     8101 gacccgcagg ctgtcctcga cggtgtcgat gtagccgttg aagaacttga cttcgttcac
     8161 cgatccgttc gggccgccct cgccactggc gccgttgtcg gagatcacca cgatgatggt
     8221 gttgtcgagc tggccggact cgtccaaata gtccaggatg cgcccgattt cggcgtcggt
     8281 gtaggacagg aagccggcga acacctctgc catccgggcg aacagccgct tctcgtcgtc
     8341 gccaagcgat tcccacgggc gcacggtgtc ctgcggcggc cacggttcgc cgttggggcc
     8401 cttgacatcc agatacgggt tgatcggcga cagctcggtg tccggcggga cgatgcccaa
     8461 ccgcttctgg ttctcgagca ccacctcgcg gtagcgctcg taacccatgt cgaacttacc
     8521 cgcgtacttg tcgacccatt ccttgaagac gtggtgtggc gcgtgcccgg cgcccgggca
     8581 gacgtaggag aaccacggct tgtcgggtgc gatcaccttg gcgtcgcgga tgaattcgat
     8641 tgtcttgtcg gcgatgtcct tggacaggtg gtagccgtct tccggaagcg cgggcggcgc
     8701 aacggggtgg ttgtcgtaga cgagttcggg gtaccactga tcggtctcgc cgcccatgaa
     8761 tccgtagaac cgctcgaacc cccgcgacag cggccaatgt cgtttggtgg ccgccagatt
     8821 cgactcctcc aacggcgtca gatgccactt gccgacgcag taggtgttgt agccgttctc
     8881 gaccaacacc tccgacagca gcgcagtctc gtcggggatg cgcccgttac agccgggaaa
     8941 gccgtcggtg aactcctcga tggtggccat tccgacggtg gtgggattgc gcccggtcag
     9001 cagcgaggcg cgcgtgggcg agcacaacgc ggtcgtgtgg aactgcgaga gccgcacacc
     9061 ccgttcggcg atgcgactca tggtcggcat ctccaccaag ccgccgaagc agtcccacgt
     9121 cgcgatgccg gtgtcgtccc acacgagata caagacgttg ggcgcccccg ccggtgccgt
     9181 cggcgcggcg tacgggcccc agtccggctc ggaatcccga atgtccagct cgatcttgcc
     9241 gttgaattcc gtcgccatgg cagcctcgtc ccgggtatct gatcgccgag tcttcgacgg
     9301 tccccggaga ttacacccgc cgtgtagccg ttagctgaga aattgacgac ggtggcagta
     9361 cgtgtcgccg gttcagccac cattgcacgg tgaagaacgg aatcgtccag cccagccacg
     9421 cacccacgcc ggccacgaac cacacgaagt gctcttcgtt gccgccaaag acgctgtcct
     9481 gcaacggctg gcacgtgatg atcagcaccg gggtccagat gcggttggtg atgatggata
     9541 aggcaagggt cgcgctgcgc accatgtggc gccgatgctg cgcgaaccgc cgctgccgcg
     9601 cggcgacgta gccgttgatc gtgaaccaca gccacagcga tgcgagcaag acgttgctga
     9661 cggccaggaa tgggccgaac ggcgtggccg cgccgatcac catggcgcac accgcggcgg
     9721 ggaccgccgc gtacacgtag acccgcccga tccgtcggtg cagtgccggc ctccgggcgc
     9781 gcaggcgggg ccagatctgc gcgacggccg tcaggatcgc gacgctcgcg aacatcacgt
     9841 gggccaccag cagcgggtag tgcagcccga atgtcgcggg cacccgcgtg ccgcccgtga
     9901 aatagggcgg cagcgagaag gccaggaacg cgacgacgac aacagcgagg gcaggggccg
     9961 gccatctgcg gcctctcgtg gttggtgcgt atgccatacg cattagcgta cggtatacgc
    10021 acatgcggtg tatagcgatt acgctggcac gtgatgacga ccgacgagcc gctgccccga
    10081 gcgctggaga tcctgtggcg cgacgggccg gcgacccggc gcacccgcgg gctgaaccgc
    10141 gaacgcattg tcgcggcggc catcgagttg gccgattccg acgggctggc cgcgctgtcg
    10201 atgggtcggc tggccgaacg gctgaagtgc ggaaccatgt cgctgtaccg gcacgtggcc
    10261 aacaaggacg aacttctcat gttcatgttg tccacagcgc ctgatccacc ccccaacacg
    10321 aatcgcacga attggcgtgg cgcgcttacc gattgggcct gcgggctgtg ggacgtctat
    10381 caccgacacc catgggtgct gcaggccacg gcggcagccg ggcctcccgc ggatcccgga
    10441 cagttggcgt ggctcgatgc cggcctggcc gcgttagagg ccaccgaact agccgaacgt
    10501 gacaagctcg ccgccgtgat ggccgtgctg cacttcgtgc gcggcgccgt ggcgctggat
    10561 atcgagggcg caccggacga cgcgcccgag taccccgcac tgctgcgacg ggtgctggac
    10621 cccgcacggt ttccggcgtt ggcggcggca gtcgaggcag gggtcttcga cgacgccaac
    10681 gacgatcatc tcgccgagtt ccgctccggt ctggaccaac tgctggacgg catcgacgcg
    10741 aaagtcggcc ggcgctagct gtcatatctc gtcgtcgcgc ctcgtcaagg acatatggat
    10801 accggaccga tcacccttat caatgtcttc gaaatcgact ctcgcgacgt tgaattgttt
    10861 ctgcgggaat ggcaggaacg cgcccagttc atgggcagac aaccgggctt ccgctcgcta
    10921 cggcttcacc gcgcgctgaa tccagactcg agatttcagt tgatcaacgt cgccgagtgg
    10981 gacagtcccg aggcgttgca aacggcgaca gcgcagggct tcttccagca gagcgcgcag
    11041 cgctccgtgc aggacttcgc cgttaccgcc catcccgcgg tctaccgcgt cgtcatcgaa
    11101 gccaccgcgg agtagcgatg ccaagaccgt atcgagtcgg cctctttgtg cgagccaaca
    11161 atgccgtgat gcgatcgctc atccgagcag gcgccaggat cggaacattc gctgtcctga
    11221 cggtgcccgg ccgcagaact gggcgcccgg tgcatgttcc actcgtcgtg ttctccgacg
    11281 gcggcaaccg ctatctggtt gcgtcgtacg gaattgtcaa ctgggtgcgc aatcttcgcg
    11341 ccgccgacgg ccgggcagaa ttgcgccgcg gccatacaac cgagaagatc acgacggtcg
    11401 aactgcctcc cgaacaagca ggcccgatcc tgcgcatcag cctgcagagc ggcccgcctg
    11461 gcataccgcg gccgatcgtc cgcatcttcc ggcgcttctt cgtgctgccg tatctcgacg
    11521 tcgacttgaa ctcgtcggtc gacgactttg tgagatcagc cgtcaaccac ccagtgttta
    11581 gagtgcagcc cggcgtgcaa agttaagaac ggctgtctgg tccgtgatct cacggtagct
    11641 agtggcgcgg tagatgtgcg agaacatcgg ggcggtggga gcatgccgag agtgacgatc
    11701 agcaagccac agacgatctc ttatccggcg cccggcgcac gcccgtaccg tcaagacgac
    11761 atcgcggtcg acccgcacgt ggtcgtgctc ttcggtgcga cgggtgacct ggcgagacgc
    11821 aagttgatac ccggcctggc ctatctggac cagtccgagc tcgcgccgca catcgaaatc
    11881 gtcgccacct cgcttgagga catctccacg gaggagtttc tcgaggtggc caaggcggcg
    11941 atcgacgact tcgggacgca caaactcacc aaggagcaat ggtcccgttt cgccgaaacg
    12001 gtcacgtatg tgccgcagag cgccggcccc gaagcgctcg ccaccgcagt cgccaacgcg
    12061 gagacgaagc tcggatccgg cgcccgtcgg ctgcactatc tgtcggtgcc gccgaaggcc
    12121 gcgcgtgccg tgatcgaaat gctgcgggac gcggacctcg ttgaccactc gcgcgtcgtg
    12181 atggagaagc cgttcggcac cgatctggcc agcgccgtcg cactcaacga cttcgtccac
    12241 gagacgttcc gcgaagacca gatcttccgc atcgaccact tcctcggcaa agaggccgcg
    12301 ctgaacatct tggcgttccg cttcgcgaac ggcctgttcg aaccgatctg gaatcgcaat
    12361 ttcatcgacc acatccagat cgacatcccc gagaccctcg gccttgacga acgcgcgaac
    12421 ttctacgaga gcacgggtgc ctacaaggac atggtggtca cccatctttt ccaggtgatg
    12481 gcgttcgtgg tgatggagcc gccgactgcc ttggagcccc gcgccatcag cgaggagaaa
    12541 aacaaggtgt ttcggtcgat gctgcccgtg gacccggaca atgtggtccg cggccagttc
    12601 accgggtacc gcgaattaga tggtgtggca agggattccg acaccgagac gtttatcgcc
    12661 ctgaaggttg gcattgacaa ctggcgatgg gcgggcgtcc ccgtctacct gcgcacgggc
    12721 aagcggatgg ccgagggcat gcggatcatc tcgatcgcct tcaaggaggc gccgcgcacg
    12781 atgttcgcgc ccgggtccgg tgtcggcgct cacggcccgg accatctgac gttcgacctc
    12841 gccgataact ccaaggtgtc gctgtcgttt tacggcaagc gaccgggccc cggcatgaag
    12901 ctggacaagc tgtcgatgca gttctcgacg caggaggttg acaccgtcag cgatgtgctg
    12961 gaggcctacg aacggctgat tcttgacgcg atgcgcggcg accacacgtt gttcaccacc
    13021 gccgagggca tcgagtcgtt gtgggagcga tcggctgacc tgctcgccga cccgccaccc
    13081 gccaaggcgt acccgcaggg gacatggggg cccaacgcga tccaccagct gatcgcgccg
    13141 aacggatggc ggctaccgtt cgagcgggcg tggcgcgagg ccaagaacaa cgactcgtag
    13201 gcggctcggc gaaacaacaa aagcccccgg ggtatccggg ggcccttgtg aaagcgactg
    13261 actacttggc cttctcgagg atctcgacga gccgccagcg cttggtggcc gacagcggac
    13321 gggtctccat cagcgaaacc cggtcgccga taccggcgat gctgtcctcg tcgtgcgcct
    13381 tgaccttcgt cgtcgtccgg atgatcttgc cgtagagcgg atggctctta cggtcctcca
    13441 gctcgaccac gatcgtcttc tgcatcttgt cgctgacgac gtagccgatc cgagtcttgc
    13501 gtcgaccgcg cggcttctcc tgacgcggag tgcgcttggg gccctttgtc tctgccatta
    13561 cgattcctca cccgcgggtc cggaggccag acccaattca cgttcgcgca gcactgtgta
    13621 cacacgcgca atttcctgac gcaccgtacg aagccgacgg ttgttggcga gctgaccggt
    13681 tgccatctgg aagcgcagat tgaacagctc ttccttggac tcgcgcagac gatccttcaa
    13741 ctcgtcgtcg gtcagttcgc gaagttcgcc gggactcact cccactgcca tcagaagtgc
    13801 tcctctcggg tcacgatgcg tgccttgatc ggcagcttat gaatagcgcg ggtcagtgct
    13861 tctcgcgcgg tcttctcatc ggggtagctc agctcgaaaa gcactcggcc gggcttgacg
    13921 ttggcgatcc accactccgg cgaaccctta cccgaaccca tccgggtttc ggcaggcttc
    13981 ttggtcagcg gacggtccgg gaagatgttg atccacacct tgccgccacg cttgatgtgc
    14041 cggttgatcg cgatacgagc ggactcgatc tgccggttgg tgatgtaggc gtgctccagt
    14101 gcctggatgc catagtcacc gaagctcacc gacgtcccgc cgctggcgac gccgcgctgc
    14161 ctggggtggt gttgcttccg gtgcttgacc ttgcggggaa tcaacatgat tcagctctcc
    14221 gtattctccg tggtcgttgc ctcagcggca gcggcctcgg ttccggtcgt cgcctcagcg
    14281 accgccggcg catcttcggt gcccgtggcg gcgcggccgg cctcagtgct cgtcgcggtg
    14341 gtgcccgacg cgccacttcg acgcgggcgg gtgcccgacg gacgctcgcg gcgcggacga
    14401 tcggcgcccg acggaaccgt cgcggtcgcc tcacgcttgc caccgacgat gtcgcccttg
    14461 taaatccaca ccttcacgcc aatccggccg aaggtcgtct tggcctcgta caggccgtag
    14521 tcgatgtcgg cgcgcagcgt gtgcagcggc acccggccct cacggtagaa ctccgagcgg
    14581 ctcatctcgg cgccgccgag acgacccgag cactgcaccc ggatgccctt gacgttgggc
    14641 tggcgcatcg cggactggat ggccttgcgc atcgcgcgac ggaacgccac gcggttcgaa
    14701 agctgctctg ccacaccctg agccaccagc tgcgcctgcg actcggggtt cttcacctcg
    14761 aggatgttga gctgcacctg cttgccggtc agcttctcca ggtcggcgcg gatgcggtcg
    14821 gcctcggtgc cgcggcggcc gatgacgatg ccgggccgtg cggtgtggat gtcgacgcgg
    14881 acccggtcgc gggtgcgctc gatctccacg tcggcgatgc cggcgcgctc caaaccggtg
    14941 gccagcagcc gacggatcgc cacgtcttcc ttgacgtagt ccttgtattg cttgtcggcg
    15001 taccaccggg acttccagtc ggtggtgata ccgagccgga agccgtgggg attgattttc
    15061 tggcccacta ctccgagccc tccttcgctt cgtcagaagc ttctgcagcc ttcttgggtt
    15121 ctgcggactt cttggctgtg ctcgatgtcg ccgccttcgc ggctccggaa gccttcttcg
    15181 cgtc
//
LOCUS       DASRJA010000570        27966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1154909,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000570 DASRJA010000000
VERSION     DASRJA010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 27966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27966
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154909"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..404)
                     /locus_tag="VFC01_25120"
     CDS             complement(18..404)
                     /locus_tag="VFC01_25120"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN82932.1"
                     /translation="MNTGLDDLAASGSLPAVVSNYLAASDRDDIDAIVACFTEDAVVL
                     DEDRHWRGAEAIRQWRESVATAYEYTVATIGSQALSEVDGGQRYEVYTHLEGNFPGGQ
                     VDLTNRFTLRGDLIAALEIVPTAVTS"
     gene            507..1067
                     /locus_tag="VFC01_25125"
     CDS             507..1067
                     /locus_tag="VFC01_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019925427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82933.1"
                     /translation="MAERIRLEDRECPLSATLGLVGEWWTLLILHDAFDGYTRFDQFQ
                     DNLGISSSLLTSRLKRLVDVGLLERRRYQTKPVRHEYVLTELGRSLRPVIVALAAWGN
                     ARLDPSQRSMVLIDSETGVEAEPILVDRTTGRPVDGPDFIFTAGPAASPPFRDRYAGR
                     PTPASAPGTPRIKNDAAKPRGSTSTD"
     gene            complement(1328..1540)
                     /locus_tag="VFC01_25130"
     CDS             complement(1328..1540)
                     /locus_tag="VFC01_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019734831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82934.1"
                     /translation="MRTNAEDVPLTEDKPEADVAEQQIPIDRTADEAGLDPTHIANRS
                     DAEANLADLIDQAITVPLADDDHPVQ"
     gene            complement(1664..3361)
                     /locus_tag="VFC01_25135"
     CDS             complement(1664..3361)
                     /locus_tag="VFC01_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005259204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN82935.1"
                     /translation="MRTMSEVATDQRASTPPAETPDIYGAYPRLSDDQIATLEAGGAR
                     RAVDTGETLVREGERSDYFFVILSGKVAVTTTDDAGNRHIIRVHGPGRFLGELGDLEG
                     QAAFYTAEVVEPGEVLVVPTERVRELVAHDPLLSDLILRAYLLRRSLLIQEGTGFRII
                     GSCYDPDTLRLREFAARNRLPHRWIDLERDKHAERLLQRFGVSPRHTPVVIWGGEVLR
                     NPTNTELARRVGLSVPDTVPDESDVVVVGAGPAGLGAAVYGSSDGLITAAMERVATGG
                     QAGTSSRIENYLGFPGGISGADLAERAALQAGKFGARIIVSAEVTRLESDGGHHLLRL
                     ADDASVVARAVVLATGARYRRLAVPGIESYEGNGVYYAATFQEALMCGTGPVVIVGGG
                     NSAGQAAIFLAARVSQVYVVIRGDDLNTSMSRYLVDQINRHPRVTVRSCTEIREVHGD
                     GALTAVVTEDKRTGEHQTIQTRALFVFIGADPNTSWLAGAIELDDHGYVPTGPAALYS
                     DTDRNQGRTQRQPMTLETSRPGVFAAGDVRRGSVKRVASAVGEGSMAIRQIHEYFGT"
     gene            complement(3345..3608)
                     /locus_tag="VFC01_25140"
     CDS             complement(3345..3608)
                     /locus_tag="VFC01_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005561811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HZN82936.1"
                     /translation="MTSVGIDPHVAAIRQVRPHTDGCEECLRHGTPWVHLRLCLTCGH
                     VGCCDSSPMRHARAHAHTVGHPIVQSMEPGPTWRWCYVHENYV"
     gene            complement(3605..4057)
                     /gene="trxA"
                     /locus_tag="VFC01_25145"
     CDS             complement(3605..4057)
                     /gene="trxA"
                     /locus_tag="VFC01_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005259202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZN82937.1"
                     /translation="METDILTCPHCGKRNRVPAAAAGKPRCANCHQWLPWIAAAGDAD
                     FADVAEKSSVPVLVDLWATWCGPCRMVSPALEQLATERAGQLKLVKVDVDKAPTISQR
                     FTVQAVPTLLLLDHGQILARQAGAAPVAALRNWLDKALSADSAKEARS"
     gene            complement(4071..6695)
                     /gene="clpB"
                     /locus_tag="VFC01_25150"
     CDS             complement(4071..6695)
                     /gene="clpB"
                     /locus_tag="VFC01_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HZN82938.1"
                     /translation="MDINKLTQKSQEALAEAQSIATRMGHIEVDGEHLLMALIDQPEG
                     LVPRLLDQTGADTAALRADLERELNRRPKVSGPGATPGQVSVTRRLANLLEAAEREAK
                     RLKDEYVSVEHLVIALAEEGSASATGRILASHNVTRDSFLTALTKVRGNQRVTSATPE
                     GAYEALEKYGRDLVAEGRAGKLDPVIGRDAEIRRVIQILSRKTKNNPVLIGDPGVGKT
                     AIIEGLAQRIVRGDVPEGLRDKTIFSLDMGSLVAGAKYRGEFEERLQAVLSEVKAGEG
                     QILLFVDELHTVVGAGATEGSLDAGNMLKPMLARGELHMIGATTLDEYRKHIEKDAAL
                     ERRFQTVLVDEPDVEDTVSILRGLRERLEVFHGVKIQDAALVAAATLSHRYITDRFLP
                     DKAIDLVDEGCARLRTEIDSMPAELDEITRRVTRLEIEEAALAKETDPASKSRLEELR
                     KELADLRAEADARHAQWDAERQAIRRVQELRGQLEQLRHEAEEAERNYDLNRAAELRY
                     GQITELERKLQAAEEQLQSKQGEKPLLREVVTEDEIAEIVAAWTGIPVARLQEGEREK
                     LLRLDEILHERVIGQDEAVQLVSDAVIRARSGIRDPRRPIGSFIFLGPTGVGKTELAK
                     TLAAALFDSEDNMVRLDMSEYQERHTVSRLVGAPPGYVGYEEGGQLTEAVRRKPYSVV
                     LLDEIEKAHADVFNTLLQVLDDGRLTDAQGRQVDFRNTVVIMTSNIGSHHLLDGVTAD
                     GEIKPDARDRVMAELRGHFRPEFLNRVDDIVLFTPLSMPQIERIVQLQLTELRDRLAE
                     RQIELDISPDARRMIAEHGYDPVYGARPLRRYIAHEVETKIGRALLRGDITGGGKIRV
                     TVENGELAVDYSEPALAA"
     gene            complement(6677..6994)
                     /locus_tag="VFC01_25155"
     CDS             complement(6677..6994)
                     /locus_tag="VFC01_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HZN82939.1"
                     /translation="MTASRYVLARRPGMQLDDFATRCALHPDMVRRLVALGLVACQQD
                     ARGDLRFEPSALVTVGRIQRLRTGLGLNYAAIGLVLDLLDHIEELESDSRRRRTSRWT
                     STS"
     gene            complement(6991..7905)
                     /locus_tag="VFC01_25160"
     CDS             complement(6991..7905)
                     /locus_tag="VFC01_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HZN82940.1"
                     /translation="MARDYYEVLGVSRDATADQIQQAFRTLARKHHPDVNKDPAAEER
                     FKEVNEAYHVLSDPETRKRYDRFGEDFRKVPEDWEERVGAGAGGFGGAGPSGARVRYG
                     QGFGGAGGINIEDLFGDMFGGSGGFGPIPGADQEALLELTVEEAYRGGRRQISLDGRN
                     YGVNIPAGVIDGQRIRLAGEGGRGSGDGPAGDLYLRVRIKPHPRFRLDGRDVTVDLPV
                     SPWEAVLGTTVAIRTPGGEAKVKVPQGSSTGRRLRLRGEGMPNPRGAAGDLYAEIKVM
                     VPPKPTARERELFEQLAAESTFDPRRGQ"
     gene            complement(7912..8436)
                     /locus_tag="VFC01_25165"
     CDS             complement(7912..8436)
                     /locus_tag="VFC01_25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005180075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZN82941.1"
                     /translation="MVSSTEHSKTDENDDRVDESPPEAVASQPDSDTELAKLEDRWRR
                     AVADLDNLRKRYARELDRERTTERSRVAGAWLPVVDNLERAIAHTGDQSDAVVEGVRS
                     ILEQALQVLEQLGYPRDAETGVPFDPQRHEVVGVVDHPDSAPGTVVEVLRPGYGDGSR
                     QLRPAAVVVSRREE"
     gene            complement(8443..10326)
                     /gene="dnaK"
                     /locus_tag="VFC01_25170"
     CDS             complement(8443..10326)
                     /gene="dnaK"
                     /locus_tag="VFC01_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZN82942.1"
                     /translation="MATAVGIDLGTTNSVIAAWQGGEPIVIPNAEGARTTPSVVAFTE
                     NGERLVGQLARRQSIMNPKGTIYSAKRFIGRRFDEISEEAKAVSFDVIEGDGGAARFD
                     VRGKQYSPEEISAQVLRKLVNDAGKFLGERVKEAVITVPAYFNDAQRNATKDAGRIAG
                     LDVLRIINEPTAAALAYGLDKKGHETVLVFDLGGGTFDVSLLDVGDGVVEVRSTAGDS
                     HLGGDDFDRRLVDYLADEFQRENNIDLRKDSQALQRLFEAAEKAKVELSSVTQTQVNL
                     PFVTADANGPKHLTMTINRSKFDELTHDLVERCMGPVKQAMDDAKVTANDIDEVILVG
                     GSTRIPAVQALVRRLTGGKDPNMTVNPDEVVALGAAIQAGVLKGEVSDVLLLDVTPLS
                     LGVETAGGVMTKIIERNTTIPARRSEVFTTAADNQPAVDIVVLQGEREMATDNRVLGR
                     FKLENIRPAPRGEPQIEVTFDIDANGILHVTARDKDTGAEQGITISEQSNLDQSEVDR
                     MLAEAEANRREDEQLRKQVDARNELDSAAYQVERRLAELGDSAAPHDRARAEMLVADA
                     RQAVKEDAPMDRVQSLTSELQQVLYGLQPMPSGGGNGQPSGDGRGSPVGDDDVVDAEF
                     DRG"
     gene            complement(10330..10833)
                     /locus_tag="VFC01_25175"
     CDS             complement(10330..10833)
                     /locus_tag="VFC01_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HZN82943.1"
                     /translation="MAEHSTVARSAVRPNEPARQVIATFDNYAEAERAVDYLSDQRFE
                     VNQVAIVGRELEYVEQILGRMNYGTAALRGAGSGALVGALIGWIFGLFNWIEPLISAL
                     VLAGYGLVFGAIVGALSGLLVHALQRGQRDFHSVSGLRPKHYDVVADLGVADRALQLL
                     ASGKRKE"
     gene            11195..12877
                     /locus_tag="VFC01_25180"
     CDS             11195..12877
                     /locus_tag="VFC01_25180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN82944.1"
                     /translation="MNDESHSPAQGTRQAEQASPGGLVALSRVTFDDSDAGEIFREAT
                     AAVGGLASCQVEASYRSVNGGFVRFPPSQPAHPDIERHRESGWDGHVDTRDGRWGWAF
                     PLSHRSTVNGCLVVSAASAPSKDQIQLLTILAQQTGAALAHAAMHDRDSGDAAKLTKL
                     NADLKVANRELGTTVTRLQRQTNVLEVLSTALAAGMGEQGIADALNDLTGHSVGIEDR
                     FGNLRCWAGPGQPHPYPKQLTDERELLLHELAVQTGQARIGGRVLTLVQPRAEILGVL
                     ALNDPDDTVTEDDLLALRYGSTVLALELAHQRAVAEIELNVRRDLVDDLLAGTDQDGA
                     YARADALGHDLRRPHHVVVMQTTGRTESTLTIAAGRAATALHLNYLLGRHAGLVVLLT
                     DGRPDPRALHHSISETLGRTTSVIGIGSRCTVPDDFPQSFMEARRALNIRLRSVNPEG
                     AAAFDELGFYRIIDAAHGGGEVESFVREWLGTLLDYDDSKNSELVMTLSDYLECGGNY
                     DESAAALHIHRSTLRYRLARIAELTGHDLRKVDTRFNLHAATRAWRFLNPDS"
     gene            complement(12903..13367)
                     /locus_tag="VFC01_25185"
     CDS             complement(12903..13367)
                     /locus_tag="VFC01_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZN82945.1"
                     /translation="MTLSVKGSASQAQWRPLREFEDLYAQMDRLAQSVFGGLTGDGAW
                     LPAADVVETDSAYVIEVELPGVRREDVDVNLNGDELVVTGEVKERKHEGLFRRRTRRV
                     GEFELRVTLPGYRRDGDIEASLAYGVLTVYVPKAESTKSSKITVSESGDGSP"
     gene            complement(13510..13962)
                     /locus_tag="VFC01_25190"
     CDS             complement(13510..13962)
                     /locus_tag="VFC01_25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1931 family protein"
                     /protein_id="HZN82946.1"
                     /translation="MTHPSGISVFERFFRSVASINIDKNDVRRFREFVDEQIDDIAIA
                     GRNSAKWNGRDVIAAQDLPITKGVQERMREFDKLEEAEEIRELLRQVVRQPPGDVAFA
                     EETEQLLPELFGGLSIALARSFRVVDTTVSNPSTEHWNRVFTLFRLVF"
     gene            complement(14661..15713)
                     /locus_tag="VFC01_25195"
     CDS             complement(14661..15713)
                     /locus_tag="VFC01_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN82947.1"
                     /translation="MHPSSTVRSWGWILRLLAVTVTFLAACSSSTNSGSTGLPAAPPS
                     STAPATTAGADLPALVRRVEPSVVTVLTESGVGSGIVYKDDGTIVTNAHVVAGVQDVT
                     VALADGQQVPGKVRGADEVSDLAVIQANRTGLPAATFQKPLPQVGELAVVMGSPLGFE
                     ATVTSGIISGLHRQIPGSASTGAPLVDLIQTDAAISPGNSGGAVLDAQGEVVGMSVAY
                     IPPTAGAVALGFAIPAATVVDVADQLLATGTARHAYIGIQPATLTSEIARLLGISRTS
                     GVVAMQVATPGPAAEAGIQPGDIVIAFNGQETATAEELIAALRSTKPGDRVQLTVLRG
                     AKTQEITVIVAERAPS"
     gene            complement(16190..16342)
                     /locus_tag="VFC01_25200"
     CDS             complement(16190..16342)
                     /locus_tag="VFC01_25200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82948.1"
                     /translation="MFSQDDVTRLDRLLVVWGIIWRTNQTSALLCASAPAVGVVPDAV
                     AVVDVI"
     gene            16346..16729
                     /locus_tag="VFC01_25205"
     CDS             16346..16729
                     /locus_tag="VFC01_25205"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HZN82949.1"
                     /translation="MDPRVTELANTINAAHDPQIQQMQDWLNQWGNPPIPTMAPGDMQ
                     MPAHGGMPGTLSERELNALREAEVVDTNRLFLTQMIAHHEGAISLAQTEIEEGQSPPA
                     VAMARSIVITQQREIDTMKGILASL"
     gene            complement(17379..18155)
                     /locus_tag="VFC01_25210"
     CDS             complement(17379..18155)
                     /locus_tag="VFC01_25210"
                     /inference="COORDINATES: protein motif:HMM:NF022219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HZN82950.1"
                     /translation="MSTDTSSALDQPTTTAPTPKPPRDKVMRIATMTKQLLDEVRTQT
                     LDTTGLKRLRTIDTQIVGELLNDLTPDLREELQRLALPLTRHTELSDAELRLAHAQLV
                     GWLQGFLKTAQTALSDPHAVLATDWITGQAAEDTSTPSDIPTSPNHPDQPHVDRCIST
                     SCLPDDTAPDEDKPAIRSARQTLITAITKRIAQQRLTAAQAAAVLHLTGPRVTQLLQA
                     DIDEFTLDELVNLLPALQLTIQIAPAPAHDIGHSRQQGWP"
     gene            complement(18221..18607)
                     /locus_tag="VFC01_25215"
     CDS             complement(18221..18607)
                     /locus_tag="VFC01_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HZN82951.1"
                     /translation="MARQNPDPYLVLGVSPTATQAEITHAYRTRLRAHHPDTRHTPPS
                     QTANDELRQVLAAYALLRDPARRADYDRATAHAATPPHRPQGSTPVERRPAGRVQIPV
                     TYRTTTPAAADVSAPPLRAGPVRRHR"
     gene            complement(19018..19320)
                     /locus_tag="VFC01_25220"
     CDS             complement(19018..19320)
                     /locus_tag="VFC01_25220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82952.1"
                     /translation="MATSTAWVTSLLDEAAAIVEAEWIRLQQDEALWERELADLPAEL
                     PAARPGPPRVGVTTTTGRWPGPPMPEDRRRWPTRRWPATPVWATQRSPPSPRETIR"
     gene            complement(19380..19799)
                     /locus_tag="VFC01_25225"
     CDS             complement(19380..19799)
                     /locus_tag="VFC01_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007298672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HSP20 family small heat-shock protein"
                     /protein_id="HZN82953.1"
                     /translation="MLMRTDPFRDFDRLAQQVLGTAARPAVMPMDAWREGDRFIVEFD
                     LPGVDEESLDLDVERNVLTVHAKRPDLDSDREMVSAERPRGVFSRQLFLGETLDSDAI
                     EASYSGGVLRLTIPVAEKAKPRRIQITSDTEKQAINA"
     gene            19933..20283
                     /locus_tag="VFC01_25230"
     CDS             19933..20283
                     /locus_tag="VFC01_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN82954.1"
                     /translation="MPDDFLARSARGVYGISVASELSGIDPQTLRLYERRGLLTPART
                     DGGTRRYSDDDLQRLQRINELVAQGINIAGIAQILHLEHRTTELESDNSDLKSENARL
                     RSEEPTTRRRRGTR"
     gene            20280..20504
                     /locus_tag="VFC01_25235"
     CDS             20280..20504
                     /locus_tag="VFC01_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019734831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82955.1"
                     /translation="MTIYLDDIPLADDVPAADAAEQLQPADVSVEDAGLDTRYVANLI
                     QRDANPADVIDQAIIVPIPDDDRDVDTALF"
     gene            complement(20501..20848)
                     /locus_tag="VFC01_25240"
     CDS             complement(20501..20848)
                     /locus_tag="VFC01_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HZN82956.1"
                     /translation="MSAGGECEADKVGRSQDPRRAGARILDTAEGVLIALRGCSLNQA
                     FVEIVQTAKAHNVSTLSLADALVALTQNHGSQYGDDAAFLAARATWGHLLDRNRPSRG
                     ARCRREDDGVAGA"
     gene            complement(21253..22488)
                     /locus_tag="VFC01_25245"
     CDS             complement(21253..22488)
                     /locus_tag="VFC01_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HZN82957.1"
                     /translation="MTTAHDIDWPQVLAERLTTTHPDVLRELLSTFIHTLMGAEADAL
                     CGAGYGQRSTERTNQRNGYRRREFDTRTGTLDLAIPKLRHGSYFPDWLLERRKRAERA
                     LTTVVATCYLLGVSTRRMDKLVETLGITSLSKSQVSVMAKELDATVESFRTRPLDAGP
                     YTFMAADALVLKVREGGRVVNVHALIAVGVNAEGYREILGIDVTTAEDGAGWLTFLRS
                     LTARGLTGVKLVTSDAHAGLVAAIGATLPGAAWQRCRTHYTTNLMAITPKASWPWVRT
                     LLHSVFDQPDAESVAAQYDRIVDALADKLPKVAEHLEDARADLLAFTAFPKQIWRQIW
                     SNNPQERLNKEIRRRTDVVGIFPDRNALIRLVGAVLAEQHDEWAESRRYLGLDILSKS
                     RTVGNNPTEQETTPAALTA"
     gene            complement(22864..23196)
                     /locus_tag="VFC01_25250"
                     /pseudo
     CDS             complement(22864..23196)
                     /locus_tag="VFC01_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020729224.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            23241..23318
                     /locus_tag="VFC01_25255"
                     /pseudo
     CDS             23241..23318
                     /locus_tag="VFC01_25255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(23351..23713)
                     /locus_tag="VFC01_25260"
     CDS             complement(23351..23713)
                     /locus_tag="VFC01_25260"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZN82958.1"
                     /translation="MPIINAANVIALLLAVVFAGLGLLKVAAVKPMRDLAAEIHYSIR
                     ADRMIGALEVAAAAGLALSLFSAAPLGVLAAGGLVMLMGAAVGTHLQNVDAVVKAAPP
                     AALGVVAIGYLVALNWSV"
     gene            complement(23715..24218)
                     /locus_tag="VFC01_25265"
     CDS             complement(23715..24218)
                     /locus_tag="VFC01_25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZN82959.1"
                     /translation="MLPSERREFVRTHRTCVFGYRRRQDGPAMTIVYYIPTDDGELLV
                     STMADRGKARVVERDGKVSLCVLDERWPFTFLQVYADAVIDRDRDLVVDVMMAVGERM
                     SGQHPGEEARPFVNEMAERENRVVIRCRPYATFATPPRHLHVNEEAGELTHWSSASIA
                     WNAPDTE"
     gene            complement(24405..25013)
                     /locus_tag="VFC01_25270"
     CDS             complement(24405..25013)
                     /locus_tag="VFC01_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN82960.1"
                     /translation="MPPGRIVAAALELVDAQGADALSMRTLAQQLNSGTATLYRHFAN
                     RSELVAHVVDHVYGEVHLDAAELAGQSWQRSCESVVRTMYAALNRHPNVTRLMLDHTP
                     FGPNAMAIREICLSVLLDSGFAPDLAARIYATLSRYVLGFAIQFSGNPEAHAAYDAEA
                     FHTVDPSQFRATLAVADAMPVPLDVEFSFGLDLLIKGLSQIR"
     gene            25135..25962
                     /locus_tag="VFC01_25275"
     CDS             25135..25962
                     /locus_tag="VFC01_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN82961.1"
                     /translation="MVLHSATDSQFTEVDGLRLHYKRAGRGPAVLLLHGTTSSLEHFD
                     RAAAILQKSFDVIRPDLPGFGLTGPRPHGDYRVRTYAGTMVQFMKKLNIPRYAVAGNS
                     LGGNIAWNLALDHPERLRALVLVNATGYPEKSLPVGMRLARNPVVRPLMRAWMPRRMV
                     ERGLQQAVGPHSTIVDDAMVTRVHHLWNQPGNRSAFVDFVNTDQPDRSAEITRITVPT
                     LVLRSAGIDGQHFARDIPGSREAIHPDGGHLLPEEDPVWFADQLTTFLNGLTDQDVS"
     gene            25959..26855
                     /locus_tag="VFC01_25280"
     CDS             25959..26855
                     /locus_tag="VFC01_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN82962.1"
                     /translation="MKYFTVTSENRQLDDITRGRLRGSFIELSDGVTHYELRGPEEGV
                     LVVLVPGLTIPLFYWDAFADELHARGLRTLAFSAYGRGYSDRVPSRYDDALFGRQLRE
                     IIPALDLPARHHIVGTSMGALIAMGYVAEHSESVATLTLVGPAGLSDATARQQRLLRS
                     DVIATVAAKLFGRRILLGHLGHNVADPNLAPQLTAMIADTYVYHGSMFAFFSTLQNFP
                     LSGRKPLYRGIGDRRIPTMLMWGTEDKVTPISDMDTACTLLRPAETHIIDCGHMAPYE
                     RPVEVAEHLAAFAAAYTDRITP"
     gene            26852..>27966
                     /locus_tag="VFC01_25285"
     CDS             26852..>27966
                     /locus_tag="VFC01_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZN82963.1"
                     /translation="MTTPILDVLIVGAGPVGAALAIDLTRRGLTVRIIDKADHAFDGS
                     RAKGIQPRTLEVLEDLGALEEILAGGSTYPLMGIHLGPFTVPWRMMRSRKAGDDVPFP
                     DTWLIPQFRTDGALHNRLRELGVAVEFHCEIAELTQTPDAVATSVTTRDGVETITARF
                     IVGADGGGSVVRKQVDIDFVGSTDEADRVLIVDAAVDGGLSRDRWHVWPALRGRFVGA
                     CPLPHSDQFQWMIRLAPDESPPTDEAQITEVIRARTGNKRLAVHDIAWKSVFRPNIRL
                     AESYRRGRVFIAGDAAHVHPPAGAQGLNTGIQDAYNLGWKLAQVLAGAPAELLETYET
                     ERQPIAAGVLGLSTKKYSGLGKMDPSSIRRGEDEQQLS"
BASE COUNT         4697 a         9115 c         9001 g         5153 t
ORIGIN      
        1 tgacctgtag gccgcgctca tgacgtcacc gcggtgggca cgatctcgag cgccgcgatc
       61 aggtcgccgc gcagggtgaa gcgattggtc agatccactt ggccgccggg aaagttgccc
      121 tccaggtggg tgtacacctc ataacgttgc ccaccgtcaa cctcgctgag cgcttgcgac
      181 ccgatcgtcg cgacggtgta ctcgtaagcg gtggcgacac tttcgcgcca ctgccgaatc
      241 gcttccgcgc cacgccagtg ccggtcctca tcgagcacca cggcgtcctc ggtgaaacac
      301 gcgacgatgg cgtcgatgtc gtcgcggtcg gacgcggcca agtaattgga caccaccgcc
      361 ggcagcgatc ctgacgcggc caagtcatcc agacctgtgt tcatgggaac cctcctcgca
      421 gttactgcta gaatagaagc tagtgacttc taaaacagaa gttagcagct tcaaaggtag
      481 cagtcaatga gaggaacggg cgtgacatgg ctgagaggat cagactcgag gaccgggagt
      541 gcccgctgtc ggccaccctc ggcctggtcg gcgaatggtg gacgctgctc atccttcacg
      601 acgcattcga cggctacacc cgcttcgacc agttccagga caacctgggc atttcctcaa
      661 gcctgctgac cagccgactc aagcgcctgg tcgacgtcgg gttgctcgaa cggcgccggt
      721 accaaaccaa gccggtccgc cacgagtacg tgctgaccga actcggccgt tccctgcggc
      781 ccgtgatcgt cgcactcgcc gcgtggggca acgcgcgcct agacccgagc cagcgcagca
      841 tggtgctcat cgacagcgaa accggagtcg aagccgagcc gattctggtc gaccgcacca
      901 ccgggcgacc cgtcgacgga cccgacttca tcttcaccgc tggtcccgct gccagtccgc
      961 cgttccggga ccgctacgcc ggtcgcccca ccccagcgtc cgccccaggg actcctcgga
     1021 taaagaacga cgccgcgaaa cccaggggct cgacgagcac tgattgaacc gtcgcggctt
     1081 cgacgattcc ggtgccgagt ttccggccgg tggcgcgggc ccgtccgggc aacatcgcgc
     1141 cggctgcagc gtaggcgtcc agcgggtcgg actgacccgt ggcgcggcgt tgacgggggt
     1201 cggggcgatt cacctcgacc acccgcaccc ccttctcggt gagatggcag gccagcccgg
     1261 cgccgtcggt gcgcaggctt gggcggccat tcgggtttgg tggcaggtct cgcctgggtc
     1321 gggatcatta ctgcaccggg tgatcatcgt cggcgagggg gacggtgatg gcttggtcga
     1381 tcaggtcggc gaggttggcc tcggcatcgc tgcggttggc gatatgagtg ggatcgagcc
     1441 ctgcctcatc ggcggtgcgg tcgatgggga tctgctgttc tgccacatcg gcttcgggct
     1501 tgtcctcggt gagcgggaca tcctcagcgt tcgtcctgat tgctccccgt tcgtgttcgt
     1561 gcggccggcc gcggcccggt ttggtggggt gtggcttgac atgagccggg cattgtcgga
     1621 ttcgagctgg gcgatgcggt gttcaaggcc gaggatctgc cgactacgtc ccgaagtact
     1681 cgtggatttg gcggatcgcc atcgagccct cgccgaccgc ggaggccacc cgtttgaccg
     1741 atccgcgacg gacgtcgccc gctgcgaaca caccgggccg actggtctcg agcgtcatcg
     1801 gctggcgttg cgttcgcccc tgattgcggt cggtgtccga gtaaagcgcg gccgggccgg
     1861 tcggcacgta gccgtggtcg tcgagctcga tagcgccggc gagccacgag gtgttgggat
     1921 cggcgccgat gaagacgaac agggcgcggg tctggatcgt ttgatgctcg ccggtccgtt
     1981 tgtcctccgt caccactgcc gtgagtgcac cgtctccgtg cacctcccgg atctcggtgc
     2041 acgaccgaac agtcacgcgg gggtgccggt tgatctggtc gacgagatac cgggacatac
     2101 tcgtgttcag gtcgtcgccg cggatcacca catacacctg ggaaacccgc gcggcgagga
     2161 agatcgccgc ctggcccgcg gagttaccac ccccgacaat gacgacggga cccgtaccgc
     2221 acatcaacgc ctcttgaaag gtggcggcgt agtacacgcc gttgccctca tacgattcga
     2281 ttcccggtac ggcgagcctg cgataccgcg ctcccgtggc tagcacgacg gcacgggcga
     2341 ccaccgacgc gtcatcggcg agtcggagca gatggtgtcc gccgtcagat tccagccggg
     2401 taacctccgc ggagacgatg atccgcgcgc caaacttgcc ggcctgcaga gcggcccgtt
     2461 cggccaggtc ggctccagag atgccgcccg ggaatccgag gtagttctcg atcctcgatg
     2521 acgtcccggc ctgcccgccg gttgcgaccc gctccatagc cgcggtgatc agaccgtccg
     2581 aggagccata caccgccgcg cccagtccgg ccggaccagc gcccacaaca acgacgtcgg
     2641 attcgtcggg cactgtgtcg ggcaccgaca gcccgacccg gcgggccagc tcggtgttgg
     2701 tcgggttccg cagcacctca ccgccccaga tcaccacggg agtgtgtcgc ggcgatactc
     2761 cgaaccgctg cagcagccgc tcggcgtgct tgtcacgttc caggtcaatc catctgtgcg
     2821 gtaaccggtt tcgcgcagcg aattctcgca gccgcaaggt atcggggtca taacaggagc
     2881 cgatgatccg gaacccggtt ccttcctgaa tcagcagcga gcggcgtaac aggtatgcac
     2941 gcaggatgag gtcactgagc aacggatcgt gggcgaccaa ttcccgcacc cgctcggtcg
     3001 gtactacgag tacttcgccg ggttccacca cttcggcggt gtagaaggcg gcttggcctt
     3061 cgaggtcgcc gagctccccg aggaaccgcc cggggccatg tacccggata atgtgccgat
     3121 tgcccgcgtc gtcggtagtg gtgacggcga ctttgccgga cagaatgacg aagaagtagt
     3181 cggagcgctc accctcgcgg acaagcgttt cgcccgtgtc gacggcgcgc cgggcgccgc
     3241 ctgcctcgag ggtggcaatc tgatcgtcag acagtcgcgg gtacgcgccg tagatgtcgg
     3301 gggtttccgc cggcggagtc gaggcgcgct ggtcggtggc gacctcagac atagttctca
     3361 tggacatagc accaccgcca tgtcggtccc ggctccatgg attgcacgat cgggtggccc
     3421 accgtgtgcg cgtgtgcccg ggcatgccgc atcggtgacg agtcgcagca accgacatgc
     3481 ccgcacgtca ggcacagccg cagatgcacc cacggtgtgc cgtgccgcag acattcctca
     3541 cagccgtccg tgtgcgggcg cacctggcga atcgccgcga cgtgtgggtc tatacctacg
     3601 gatgtcatga ccgtgcctcc ttcgcgctgt cggccgacag tgccttgtcc agccaattgc
     3661 gcagcgccgc aacgggagcg gcacccgctt gccgcgccag tatttgcccg tggtcaagga
     3721 gcagcagtgt cggcaccgcc tgcaccgtga accgctgcga aatcgtcggt gccttatcca
     3781 cgtcgacctt gaccagtttg agttgtccgg cgcgttcggt ggcgagctgt tcgagtgccg
     3841 gactcaccat gcggcacggt ccacaccagg tcgcccacag gtcgacaagt actggaaccg
     3901 aagacttttc ggcaacgtca gcgaagtccg catcgccggc cgcagcgatc cacggcagcc
     3961 actggtgaca gttggcgcac cggggcttac ccgcggccgc cgcgggtacc cggttgcgtt
     4021 ttccgcagtg cgggcatgtc aggatgtctg tttccatggc gccttctccg tcaggccgcc
     4081 agggccggtt ccgaatagtc cacggcgagt tcgccattct ccacggtgac gcggatcttg
     4141 ccaccgcccg tgatgtcgcc gcgtaacaac gcccggccga tcttggtttc tacctcgtgg
     4201 gcgatgtagc ggcgcagcgg tcgcgcgccg tacaccggat cgtagccgtg ttcggcgatc
     4261 atccggcgtg cgtccgggga gatgtcgagc tcgatttgcc tctccgctaa ccgatcccgc
     4321 agctcggtca gctgcagctg gacgatccgt tcgatctgcg gcatcgatag cggcgtgaac
     4381 agcacgatgt cgtcgacgcg gttgaggaac tcgggccgga agtgtccgcg caactcggcc
     4441 atcacccggt cgcgcgcgtc gggcttgatc tcgccgtccg cggtgacgcc gtcgagcagg
     4501 tggtgcgatc cgatgttgga ggtcatgatg accaccgtgt tgcggaagtc gacctgacgt
     4561 ccctgtgcat cggtgagccg gccgtcgtcg agcacctgca gcagggtgtt gaacacatcg
     4621 gcgtgcgcct tttcgatctc gtcgagcagc accaccgagt acggcttgcg acgcacagct
     4681 tcggtgagct ggccgccctc ctcgtagccg acgtatcccg gtggcgcacc gaccaaccgg
     4741 ctcaccgtgt gccgctcctg gtattcgctc atgtcaagcc gaaccatgtt gtcctcgctg
     4801 tcgaacaacg cggcggccag cgtcttcgcg agctcggtct taccgacccc ggtgggtccg
     4861 aggaagatga acgagccgat cgggcggcgc ggatcccgga ttccggagcg ggcccgaatc
     4921 accgcatcgg agaccagctg gacagcctca tcctggccga tcacccgctc gtgcagtatc
     4981 tcgtcgagcc gcagcagctt ttcccgttca ccctcctgca atcgcgcgac cggtatgccg
     5041 gtccatgcgg ccacgatttc ggcgatctcg tcctcggtta cgacctcgcg cagcaagggc
     5101 ttttcgccct gcttggactg cagctgttcc tctgccgctt gcagtttgcg ctccagttcg
     5161 gtgatctggc cgtagcgcaa ttcggcggcg cggttgaggt cgtagttgcg ttcggcttct
     5221 tcggcctcgt gccgtagttg ctcgagttgc ccacgcagtt cctgtacccg ccggatggcc
     5281 tgccgttcgg catcccattg cgcgtgccgg gcgtcggcct ccgctcgcag atcggccaat
     5341 tctttgcgaa gttcctccag acgggacttg ctggccgggt cggtttcctt ggcgagcgcc
     5401 gcctcctcga tctcgagccg ggtgacccgc cgggtgattt cgtccaactc ggctggcatc
     5461 gagtcgattt cggtgcgcag ccgggcacag ccctcgtcca ccaggtcgat cgccttatcg
     5521 ggcaggaagc ggtcggtgat atagcgatgc gacagcgttg cggccgctac cagggcggca
     5581 tcctgaatct tcaccccgtg gaacacctcg aggcgttcgc gcagcccgcg cagaatcgaa
     5641 acggtgtcct cgacgtcagg ttcgtcgacc agcacggtct ggaaccggcg ctccagcgcg
     5701 gcgtcctttt cgatgtgctt gcggtattcg tcgagggtgg tggcgccgat catgtgcagc
     5761 tcaccgcggg cgagcatcgg cttgagcatg ttgccggcgt ccagcgaccc ctccgtcgcc
     5821 ccggcgccga ccacagtgtg taactcgtcg acgaacagca gaatctggcc ctcgccggcc
     5881 ttcacctccg acagcaccgc ctgcagccgt tcctcgaatt cgccgcggta cttggcccct
     5941 gccaccagtg agcccatgtc gagggagaat attgtcttgt cgcgcaggcc ttctgggacg
     6001 tcgccgcgca cgatgcgctg ggccaggcct tcgatgatcg cggtcttgcc gacgccgggg
     6061 tcgccgatga gcacagggtt gttcttggtc ttgcggctga ggatctggat caccctgcgg
     6121 atctcggcgt cgcgtccgat caccgggtcc agcttgccgg cgcggccttc ggcgaccagg
     6181 tcgcggccgt acttctccag cgcctcgtag gcgccctccg gggtcgcgga ggtgacccgc
     6241 tggttgccgc ggaccttggt cagcgcagtc aggaacgagt ctcgagtcac gttgtgggag
     6301 gcgagaatcc gtccggtggc actcgccgaa ccctcttctg cgagggcgat gacaaggtgc
     6361 tccaccgata cgtattcgtc cttgagccgc ttggcttccc gctcggcggc ctccagcagg
     6421 ttcgccaggc gcctggtgac agaaacctgg cccggcgtgg cgccggggcc gctgaccttc
     6481 ggacgccggt tcaactcgcg ttccaggtcg gcgcgcagcg ccgcggtgtc ggcgcccgtc
     6541 tgatcaagca gccgcggtac cagaccctcg ggctggtcga tcagtgccat caacagatgt
     6601 tcaccatcga cctcgatatg acccatccgg gtcgcgatgc tctgcgcttc ggccaacgcc
     6661 tcctgtgact tctgcgtcag cttgttgatg tccaccgcga tgtcctcctt cggcgagaat
     6721 cggattccag ctcttcgatg tggtccagca ggtcgagcac cagcccgatt gccgcgtagt
     6781 tcagtccgag accggtcctc aaccgctgga tgcggccgac tgtcaccaac gcagaaggtt
     6841 cgaaccggag atcgccgcgg gcatcctgct ggcaagcgac gaggccgagc gccaccagtc
     6901 ggcgcaccat gtcgggatgc aacgcgcagc gtgttgcaaa gtcgtcgagc tgcattccgg
     6961 gccgccgcgc caggacatag cgtgatgcgg tcattgtccc ctcctggggt cgaacgtgga
     7021 ctccgccgcc agttgctcga acagctcgcg ttcccgcgca gtgggtttcg gtggcaccat
     7081 caccttgatt tctgcgtaga ggtcgcctgc ggcaccgcgc gggttgggca tgccttcgcc
     7141 gcgcagccgc aaccgccggc cggtcgacga cccttgcggc accttaactt tcgcttcgcc
     7201 gccgggagtt cgaatagcga cagtggtccc cagcaccgcc tcccatggcg acaccggcag
     7261 atcgacggtg acgtcgcgtc cgtcgagccg gaatcgggga tgcggcttga tgcgcacccg
     7321 caggtacaga tctccggccg gtccatcgcc gctacctcgg ccgccctctc ccgccagccg
     7381 tatccgctga ccgtcgatga cacccgcggg aatgtttacc ccgtaattgc ggccatctag
     7441 tgagatctgc cgtctgccgc cccggtaggc ctcctcgacg gtcagctcca gcagcgcttc
     7501 ctggtcggca cccggaatcg gcccgaatcc gccactgccg ccgaacatgt cgccgaaaag
     7561 gtcctcgatg ttgatgccgc cggcgccgcc gaagccctgg ccgtagcgca cccgcgcgcc
     7621 ggagggccct gcgccgccga agccaccggc accggcgccc acccgctctt cccagtcctc
     7681 gggcaccttc cggaagtctt cgccgaatcg gtcgtagcgc ttgcgggttt cggggtccga
     7741 cagcacgtga taggcctcgt tgacctcctt gaagcgttct tcggccgctg gatctttgtt
     7801 gacgtcgggg tgatgcttgc gggccagtgt gcggaacgcc tgctggatct gatcggcggt
     7861 cgcgtcccgc gacaccccga ggacttcgta gtagtcgcgg gccacccgat ctcactcctc
     7921 gcgtcggctg accaccacgg ccgccggccg cagttgcctc gatccgtcgc cgtagcccgg
     7981 tcgaagtact tcgacaaccg tcccgggcgc gctgtccggg tggtccacca caccgaccac
     8041 ctcgtgacgc tgcgggtcga agggcacccc tgtctccgca tcgcgcggat agccgagctg
     8101 ctcgagaacc tgtagcgctt gttcgagaat gctccgcacg ccctcgacga ccgcgtcgga
     8161 ctggtcaccc gtgtgggcga tcgcccgctc caggttgtcc acgacgggca gccaggcgcc
     8221 ggccaccctg gatcgttccg ttgttcgttc gcggtccagt tcgcgcgcat accgcttgcg
     8281 caggttgtcc agatcggcga ccgcgcgtcg ccagcggtcc tcgagcttgg ccagctcggt
     8341 gtccgaatca ggttgggagg caaccgcttc aggcggactt tcgtctaccc ggtcatcgtt
     8401 ttcatcggtc ttggaatgtt ctgtcgaact gaccatggcg gctcagcccc ggtcgaactc
     8461 ggcgtcgacc acgtcgtcgt caccgactgg agagccccgg ccgtcgccgg atggttgccc
     8521 gttgccgcca ccggacggca ttggttgtag cccgtaaagc acctgctgca gttcagatgt
     8581 cagcgattgc acccggtcca tcggtgcgtc ttccttgacg gcttgccgag cgtcggcgac
     8641 caacatctcg gcgcgggccc ggtcgtgcgg cgccgccgag tcgcccagct cggcgagccg
     8701 gcgctcgact tggtaagccg ccgagtcgag ttcattgcgt gcatcgacct gcttgcgcaa
     8761 ctgttcgtcc tcgcgacggt tggcttcggc ctcggcgagc atccggtcga cctcgctctg
     8821 atcgaggttg gactgttcgc tgatagtgat gccctgctcg gcgccggtgt ccttgtcgcg
     8881 tgcggtgaca tgcaggatgc cgttggcgtc gatgtcgaag gtgacctcga tctgtggctc
     8941 accgcggggg gccgggcgga tgttctccag cttgaacctg ccgagtacgc gattatccgt
     9001 ggccatctcg cgttcacctt gcagcacaac gatatccacg gcgggttggt tgtccgccgc
     9061 ggtggtgaac acctcgctgc gccgggccgg gatggtggtg ttgcgctcga tgatcttggt
     9121 catcacgccg ccggcggtct ccacacccag cgagagtggg gtgacatcga gcagcaagac
     9181 gtcggagacc tcgcccttga gcacgcccgc ctggatcgcg gcgcccagcg cgaccacctc
     9241 gtccgggttg acggtcatgt tcgggtcctt gccgccggtg agccggcgga ccagggcctg
     9301 gacggcgggg atgcgggtcg acccgccgac caggatgacc tcgtcgatgt cgttggccgt
     9361 cactttggcg tcgtccattg cctgcttgac cggacccatg cagcgctcca ccagatcgtg
     9421 cgtgagttcg tcgaacttcg atcgattgat ggtcatggtg aggtgcttgg ggccgttggc
     9481 gtcggcagtg acgaacggca ggttgacctg cgtctgtgtc accgacgaca gttccacctt
     9541 cgccttctcg gccgcctcga acagccgctg cagcgcctgc gaatctttgc gcagatcgat
     9601 gttgttctcg cgctggaact cgtcggccag atagtccacg agccggcggt cgaagtcgtc
     9661 gccacccaga tgggaatcac ctgcggtgga gcgaacctcg accaccccgt caccgacgtc
     9721 gagaaggctg acatcgaacg tgccgccgcc gaggtcgaag accagcacgg tctcgtggcc
     9781 ctttttgtcc agtccgtagg ccagcgccgc ggcggtcggc tcgttgatga tgcgcagcac
     9841 atccagcccg gcgatccggc cggcatcctt ggtggcgttg cgttgcgcgt cgttgaagta
     9901 cgccggaacg gtgatcaccg cttccttgac ccgttcaccg aggaacttgc cggcgtcgtt
     9961 gacgagtttg cgcagcactt gcgcgctgat ctcttcgggc gagtactgct tgccgcgcac
    10021 gtcaaaccgg gcggcgccac cgtcgccctc gatgacgtcg aagctgaccg ccttggcttc
    10081 ctccgatatt tcatcgaagc ggcgaccgat gaatcgtttc gccgagtaga tggtgccctt
    10141 cgggttcatg atcgactgcc gcctggccag ttgcccgacc aggcgctcgc cgttttccgt
    10201 gaaggccacc accgacgggg tcgttcgcgc gccctcggcg ttgggaatca caatcggctc
    10261 cccgccctgc caggcggcga tcaccgagtt cgttgtcccg agatcgatgc ctaccgctgt
    10321 tgccatcgtc tattcctttc gtttgccgct ggcgaggagt tgcagagcgc gatcggcaac
    10381 ccccaagtcc gcgacgacgt cgtaatgctt gggtcgcagc ccactaacgg agtggaaatc
    10441 gcgttggccg cgctgcagcg catgcacgag cagcccggac aaggcgccca cgatggcgcc
    10501 gaagaccagg ccgtagccgg ccagcaccag ggcggagatc aatggttcga tccagttgaa
    10561 cagcccgaag atccacccga tcagcgcgcc gaccagggcg ccggagccag caccgcgcag
    10621 cgcggcggtg ccgtagttca tccggccgag gatctgttcc acgtactcca gttcccggcc
    10681 gacgatggcc acctggttta cctcgaaacg ctggtcggac aggtagtcga ccgcgcgttc
    10741 ggcttcggcg tagttgtcga aggtggcgat cacctggcgg gcgggctcat tgggccgcac
    10801 cgccgatctc gcaaccgtcg aatgctcagc catcactccc cctatccctg taggccatac
    10861 gcactaaaac catggcttcc ccgaagtggc gaaagtcgtt acacggaagc gccgacacca
    10921 ggggaaagca gcgcacaccc tgtaacgcgc cgtaattcgc tcccaaacga ggagtctggc
    10981 aacccgtagc ggctgtcgcg tgctcccggt gagccgaaaa tctggactct gattcgtcct
    11041 gtcagcacca ccctggagta gggctgcgtt tcggacgccg ggtgggcact ctcggacccg
    11101 gaaaggcggt tcggaacgcc aacttcgggt atgccgttcc tcgtgacgcc ttagcggacc
    11161 cctccggccg cggtggctcg agagccactc caacatgaac gatgaatcgc acagcccggc
    11221 acagggcacc cgccaggccg aacaggcttc gcctgggggt ttggtcgcgc tgtcgcgtgt
    11281 cactttcgat gactccgacg ccggcgagat cttccgcgaa gcgaccgccg ccgtcggcgg
    11341 actcgcatcc tgccaggtcg aggccagtta ccggtcggtg aacggcggat tcgttcgctt
    11401 tccaccatcc cagccggcac accccgacat cgaacggcac cgcgagtccg gctgggatgg
    11461 ccacgttgac acaagagacg gccggtgggg gtgggcgttc ccgctgagtc accgaagcac
    11521 cgtcaacggg tgtttggtgg tcagtgccgc cagtgcaccg tcgaaagatc agatccagtt
    11581 gctgaccatc ctggcccagc agaccggcgc cgcgctggca catgccgcaa tgcatgaccg
    11641 cgactccggt gatgcggcga agctgacaaa attgaacgcc gatctgaagg tagcgaaccg
    11701 tgaactcggc acaacggtga cgcggctaca gcggcagacg aacgtgctcg aagtattgag
    11761 cacggcgctg gccgcgggaa tgggcgagca aggtatcgca gatgcactga atgacctgac
    11821 ggggcattcg gtgggtatcg aggatcggtt cggcaacctg cgctgttggg ccggacccgg
    11881 acaacctcac ccctacccca aacagctcac ggacgagcgc gaactcctac tgcacgagct
    11941 ggccgtacaa accggtcagg cacgaatcgg agggcgagta ctcacacttg tccagccacg
    12001 ggccgagatc ctcggtgtcc tcgctttgaa cgacccggac gacacggtga ccgaggacga
    12061 cttgcttgcc ctgcgctatg gcagcaccgt tttggcgctg gagcttgccc accagcgagc
    12121 cgtcgctgag atcgaactca atgtgcgccg cgatcttgtc gacgatctgt tggccggcac
    12181 cgaccaggac ggggcctacg ctcgtgccga cgcccttggc catgacctac gacggcccca
    12241 ccatgtggtg gtgatgcaaa ctaccggtcg caccgagagc acgctgacaa tcgccgccgg
    12301 gcgcgccgcg accgcgttgc atctgaacta cctgctcggg cgtcatgcag gtctggttgt
    12361 gctgctcacc gacggccgcc cggacccgcg ggcgttgcac cactcgatca gtgaaaccct
    12421 tggcaggacg accagcgtga tcggcatcgg ctctcgatgc acggtgcccg acgattttcc
    12481 gcagtccttc atggaagcgc gtcgcgccct caatatccga ctgcgctcgg tgaacccgga
    12541 gggcgcggcg gcgttcgacg aactcggttt ctatcgcatc atcgacgccg cccatggcgg
    12601 cggcgaggtc gaaagtttcg tgcgcgaatg gctgggcaca ctcctggatt acgacgacag
    12661 caagaactcc gaactggtga tgaccctgag cgattacctg gagtgcgggg ggaattatga
    12721 cgaatccgcg gctgcactcc acattcatcg cagcacgctt cgttaccggc tggcgcgcat
    12781 cgctgagctc accggccacg acctccgcaa ggtcgatacc cggttcaacc tgcacgccgc
    12841 gacgcgggcg tggcgattcc tgaaccctga tagctgaccg gaaacccgct gcaacggggc
    12901 gtctagggtg agccgtcgcc cgactcactc accgtgatct tgcttgactt ggtgctctcg
    12961 gccttcggta cgtaaacggt gagcacgcca taggccaacg acgcctcgat gtcgccgtcc
    13021 cgacggtagc ccggcagcgt gacgcgcaac tcgaactcac cgactcgtcg cgtacggcgg
    13081 cggaacaaac cctcgtgctt gcgctccttc acctcgccgg tcaccaccaa ctcgtcgccg
    13141 ttcagattga catcgacgtc ctcgcgccgc acaccaggaa gctcgacctc gatgacatac
    13201 gcgctatcgg tctcgacgac gtcggccgcc ggcaaccagg caccatcacc ggtgaggcca
    13261 ccgaacaccg actgcgccag ccgatccatc tgcgcataga gatcctcgaa ctcgcgcaac
    13321 ggacgccact gcgcctgact agcagaacct ttcacggaca gagtcatgtc agctcccctt
    13381 ctaattcatc agcggctgtt gtcaaagact gccccgtcga tgcgggcggg cgcctcgcac
    13441 tgtcgggacg atttcaggcc cgcgatttca cacgctgggg tcactcacga tcggtgggga
    13501 ccgtcggcat cagaacacca gccggaacag cgtgaacacc cggttccagt gctcagtcga
    13561 cgggttggac accgtcgtgt ccaccacgcg aaacgaacgg gccagcgcga tactcaaccc
    13621 gccgaacaac tctggcagca gctgctcggt ttcctcagcg aacgcaacgt ccccaggtgg
    13681 ttgccgcacc acctggcgca gcagctcacg gatctcctcg gcctcctcga gcttgtcgaa
    13741 ctcgcgcatt cgctcctgga cgcccttggt gatcggaagg tcctgcgccg caatcacgtc
    13801 acgcccgttc cacttggccg agttgcgccc cgcgatagca atgtcgtcga tctgctcgtc
    13861 gacgaactcc cggaagcgac gtacgtcgtt cttgtcgatg ttgatgcttg cgaccgagcg
    13921 gaagaaccgt tcgaacaccg aaatacccga cggatgggtc actcgatacc tccgttccaa
    13981 agatccagac tggcccgagc aggccaacac tcaacgttgg cactgttgtc ggtgaccccg
    14041 aaccggaccc gcaggtgcaa tctcgccagg ccggttcggc ctcacaggtc aagaggcacc
    14101 agggaacgca cgtcggacga tgtagcggac aagccgcttg ccgcgcgcac atgctgtttc
    14161 tgcgtgccta ccctcagcgg tgtttttcga tccgccggtc cggtatcgac atcaccggat
    14221 tatccagcag cgcaacggtt ctggtgtgca gagcgtcccc ctctcggcct gtaatgtgtg
    14281 cccgcacagt tgctcaccca ggtcacctcg caacgcctcg cgcagccgtg ccacctcctc
    14341 cagcgccgca tcgtcgaccg gcttaccaga ccggccccac agcaccgtgc gcagctttct
    14401 ctgcggtgcg cgccgggtcg ggcgttcttc gtggcctggt tgagagccgg ttcgggccca
    14461 ccaatccacc ctgcactgtt gttcagacat tccccgcggt gtcggtatgg tcggcggcgt
    14521 cgtgtgatcc gcgtgcaggg gcgtgtctga tgacggcggg ggcggaccgg gcgcgaggtg
    14581 acattgggga ctgagtcgtt cgcagcgtgt ggaccggtcg tggggagaca tcaaatttga
    14641 tacgaacgag aagatcggct ctagctgggg gcgcgctccg caactatgac cgtgatctct
    14701 tgggtcttgg ctccgcggag cacggtcaac tggacgcggt cgcccggttt ggtgctgcga
    14761 agtgcggcta tgagctcctc tgccgtcgcg gtttcctgac cgttgaacgc gatgacgatg
    14821 tcacccggtt ggatgcctgc ctccgctgcc ggacctgggg tcgcgacctg catggcgacc
    14881 acaccactag tgcggctgat tcccagcagc cgggcaatct ccgaggtcag ggtggcgggc
    14941 tgaataccga tataggcgtg gcgggcggtc ccggtggcca acagctggtc ggcgacgtcg
    15001 acgacggtcg cggcggggat ggcgaaacct agggctaccg cacccgctgt cggtggaatg
    15061 taggccacgc tcatacctac aacttcgcct tgcgcatcga gtactgcacc cccagagtta
    15121 cccggcgaga tggcggcgtc ggtctggatt aggtctacca acggtgcgcc ggtggaagcc
    15181 gacccgggga tctggcggtg tagtcccgag atgatgccgg atgtaactgt ggcctcgaat
    15241 cccagcgggc tgcccatgac tacggcgagc tcaccgacct gcggcagcgg cttctgaaat
    15301 gtcgcagcgg gcaggcccgt acggttggct tggatgacag cgaggtcgga aacctcatcg
    15361 gcgccacgaa cctttcccgg cacctgctgc ccatcggcga gggctacggt gacgtcctgc
    15421 acgccggcga ccacatgagc attggtgacg attgtgccgt cgtctttata gacaatgccg
    15481 ctgcccacac cgctttcggt gagcaccgtg acgaccgagg gctcgacccg tcggaccagt
    15541 gccggtagat ccgcccccgc agtcgtggcc ggggccgtcg aactgggggg tgccgcggga
    15601 aggccggtcg agccggagtt ggtcgagctg ctgcatgcgg cgagaaaggt gaccgtgacg
    15661 gcaagcagcc gcagtatcca accccaggac cgtacggtcg acgatgggtg catcagcttt
    15721 tcgcctccgt ttccacacaa tgacttccgt gccggtaacc gcggcccagc actccgccag
    15781 gcagcgcccc tacgggatcg acgcgcaccc cgacgaattt cggtcgggtg tcccttcggg
    15841 gccacaccag cccgttaccg acgcggccgc ttgtataaga accttcgcac ctcaacgccg
    15901 agatcgacat ggtcctgtca gtcccaagcc caatggacgc acctggcccg acaggccgaa
    15961 gaaacccaac tcatgctgcg ccaaagtgtt tccgtgtcgg gtgtgcgagt cgccgtgatg
    16021 acgttgaatc gaccgcgtgt gagagggcga cccggggcca gatcacgaca aggagggctc
    16081 acatcgggac gcggaaacag atcattttga tcggcgctag cgccgtggcg gtgttggtcg
    16141 tctccgccgc ctgcaacaag gtcgacaccg gccgccagga ccccgcgggt cagattacgt
    16201 cgacgaccgc caccgcgtcc ggcacgacgc cgacggcagg cgccgaggcg cataacaatg
    16261 ccgacgtctg gttcgtgcgc cagatgattc cccacaccac cagcaggcga tcgagacgag
    16321 tgacatcgtc ctggctaaac acggcatcga tccccgcgtg accgaattgg ccaacacgat
    16381 caacgccgca cacgatccgc agatccagca gatgcaggac tggctcaacc aatggggcaa
    16441 tccacccata ccgacgatgg cacccggcga catgcaaatg cctgcacatg gcggcatgcc
    16501 gggcacgctg tctgagcggg aattgaacgc gttgagagag gccgaggtcg tcgacacaaa
    16561 caggctgttc ctgacacaga tgattgcaca ccacgagggc gcaatcagct tggcgcagac
    16621 cgaaatcgag gaaggtcagt ctccaccggc ggtcgcgatg gcgcggtcga tcgtcatcac
    16681 ccagcagcgg gagattgaca ccatgaaggg cattctcgcc tcgttgtagc tacgtcatca
    16741 tcccgcgaag gccgggacaa tttcagggtg ccagcggtgg cttcatcgag caacgcctcg
    16801 cgtcggctgc gccacgccgc ctggcgggaa ggtcttggac ctgaaaccgc cgccacacgt
    16861 gcgcgttcct gggcggcggc gtgtatggaa cgaacccggc cccctcgtcc cactcggcgc
    16921 ccagccggtc aagggtcttg atgtcggccg gctcggtcgc gtttatctgt aggccctgtc
    16981 ggggtgtatt cgactgtgtc tcatggtgtg tgatgagtcg tgtttgtcca ggggaacact
    17041 tttatctgcg gggctgtgtc ttgtgatcgt gggcagcccg cggcgtcctc atccaactcg
    17101 acgcactacc cgagcgaggt gatcatgacc gcccgtcgtc atgggagggt gggtggccaa
    17161 cccgagacga gatcacccaa gccgtcagga atctgtggac cggcgacccg tggcagtaca
    17221 ccgagatcac cgtcagggtg ctgggtgaag ttgagaacat tcgcgcgtgg aacgaaaaag
    17281 ctgacacgtg aggggagccg ctgttagttc cagtaactgg gggctccggc gcgattgcgt
    17341 tcgggtgtgc tgggcgacct ggtgggcgtg ttgaatcgtt agggccatcc ttgttggcgt
    17401 gagtgcccga tgtcgtgtgc tggggcgggg gctatttgaa tggtcagttg cagggcgggg
    17461 agcaggttga ccagttcgtc gagtgtgaat tcgtcgatgt cggcttggag gagttgggtc
    17521 accctcgggc cggtgaggtg gagaacggcg gctgcttgag ccgcggtgag gcgttgttgt
    17581 gcgatgcgtt tggtgatggc ggtgatcagt gtttggcgcg ctgatcggat cgctggcttg
    17641 tcctcgtcgg gcgcagtgtc atcgggtagg cagctcgttg agatgcatcg gtcgacgtgc
    17701 ggttgatcgg ggtggttcgg tgaggtgggg atgtcgctgg gcgtggaggt gtcttcggcg
    17761 gcttggccgg tgatccaatc ggtcgccagg acagcgtgtg gatcggataa cgcggtttgg
    17821 gctgtcttga ggaacccttg aagccagccc actagttggg cgtgggcgag gcgcagttca
    17881 gcgtcggaca gttcggtgtg gcgggtgagc ggtagggcaa ggcgttgcag ctcttcgcgt
    17941 aggtcggggg tgagatcgtt gagcagctca ccgacaattt gggtgtcaat ggttcgtagg
    18001 cgcttaaggc ctgtggtgtc cagcgtttgg gtacgaactt cgtcgagtag ttgtttggtc
    18061 atggtcgcga tccgcatgac cttgtcccgc gggggttttg gggtcggcgc tgtcgttgtt
    18121 ggttggtcga gcgcgctgct ggtatcggtg ctcatgcggt cctcggtggg gtggtctagt
    18181 gatacgggtt gtgcgccggt cgttttacgc gatcgcgcag ctatcggtgc cggcgcaccg
    18241 ggccggcgcg caatggtggc gctgagacat cggctgccgc cggggtggtg gttcggtagg
    18301 tgacggggat ctggacgcgg ccggcagggc gacgctcaac aggtgtcgac ccttgcgggc
    18361 ggtggggtgg tgtggcggcg tgggcggttg cgcggtcgta gtcggcgcga cgggcggggt
    18421 cacgcagtag ggcgtaggcg gccagcactt gccgcagctc atcgttggcg gtttgcgagg
    18481 gcggtgtgtg gcgtgtgtcg gggtgatggg cgcgtagccg ggtgcggtag gcgtgggtga
    18541 tctcggcctg ggttgcggtc ggtgacacgc ccaacaccaa gtagggatcg ggattctggc
    18601 gcgccatggc tgctatctcc gaggacatgg tgcggtgact cccaggctag gaggtcgacc
    18661 gagagcctgc caccgcggtg agatggtcca tcaaatcttc atgccgtgaa tgggccgccg
    18721 gattgtgctc ttggaagcca cgccctggcc ggggggcgat cactgctgct tcgatatggg
    18781 gaacacgccc gggcgggccg ggagccgtgc cgcggcccgc ccgggcgtgg cagcgacttg
    18841 gtgcacgctc cacttcgaat atgccaccac gagtggcggt gcgtgcgccg accgactctg
    18901 gacgctggag tcggccgcgg tgacgcgaca tctgaatcat tgtgggcgac acataggagt
    18961 cgttgcctgg tggtgatcat cggtagtggc atcacctccc ttgtccgaca gtgcctttca
    19021 gcggatggtc tcccgaggcg acggaggaga ccgctgggta gcccataccg gcgtcgcggg
    19081 ccatcgccgc gtcggccacc ggcgacggtc ctctggcatc ggtgggcctg gccatcgccc
    19141 tgtagtggtg gtgacgccga cgcggggcgg gccgggccgg gccgcaggca gctcggcggg
    19201 caggtcggcg agctcacgct cccacagggc ctcgtcctgc tgcaggcgta tccactcggc
    19261 ctccacgatc gccgccgcct catcaagcag gcttgtgacc catgcggtgc tggtcgccat
    19321 ctcacttacc cccttccccg aatgctgcgc ccccggccca gccagtgagg tggaccgtgt
    19381 caggcgttga tggcttgctt ctcggtgtcg ctggtgatct ggatccggcg cggtttggcc
    19441 ttctcggcga cggggatggt caaccgcagc accccgccgc tatagctggc ctcgatcgcg
    19501 tcgctgtcta acgtctcgcc caagaacagc tgtcggctga acaccccgcg cggacgctcg
    19561 gcggacacca tctcccgatc ggaatcgagg tcggggcgct tggcgtgcac ggtgagcaca
    19621 ttgcgctcga cgtcgagatc cagagactcc tcgtccacgc cgggcaggtc gaactcgacg
    19681 atgaaccggt cgccctcacg ccaggcatcc atcggcatta ccgccggccg tgcggcggtg
    19741 cccagcacct gctgggcgag gcgatcgaag tcgcggaacg gatcggtgcg catcaacatg
    19801 atctttccct cctaacccaa tttggctgtc tgacagatct gtggaaactg tcatagattt
    19861 cttatagcac tagagtcagc tttgtgcaag gatgattttg gatgcagtct cgtggacgga
    19921 tggagcagct cagtgcctga cgatttcctg gcgcgatctg cgcgcggcgt atacggcatc
    19981 tcggtggcct cggagttgtc cgggattgat ccgcagacgc tgcggctcta cgagcggcgc
    20041 ggactgctca ccccggcgcg caccgacggc ggcactcgcc gatacagcga cgacgatctg
    20101 cagcgtctac agcgcatcaa cgagctcgtc gcccaaggca tcaacatcgc cgggatcgcc
    20161 caaattttgc acctggaaca ccgcaccacc gaactggaat ccgacaacag cgatctcaag
    20221 tccgaaaacg cccgactgcg atccgaggag ccgaccacca gacggaggag gggaacccga
    20281 tgacgatcta tctcgacgac atcccgctgg ccgacgacgt tcccgccgcc gacgcggccg
    20341 aacagctcca gcccgccgac gtcagcgtcg aggacgccgg actcgacact cgctacgtgg
    20401 ccaacctgat tcagcgtgac gccaaccccg ccgatgtgat cgaccaagcc atcatcgtgc
    20461 caattcccga cgacgaccgc gacgtcgaca ccgccctgtt ctaggcgccg gcaacaccgt
    20521 cgtcttcgcg gcgacatctc gcgccgcggc tgggcctgtt cctgtccaac aggtggcccc
    20581 acgtggcgcg ggcggcgaga aatgcggcgt catccccgta ctgggaccca tggttctggg
    20641 tgagggccac caacgcatca gccagactca aagtgctcac gttatgcgcc ttcgcggtct
    20701 gcacgatctc gacaaatgcc tggttgagac tgcagccccg cagcgcgatg agcacgcctt
    20761 cggcggtgtc gaggatccgc gcccccgcgc ggcgagggtc ctgcgaccgg ccaaccttgt
    20821 ccgcctcgca ctcacctcct gcgctcatct tcaccccaat tgcacacaac ataacctatg
    20881 gtaatgagta catatttctt caccagtgta gtgcggagtg gccgtgatta aggaatgcgt
    20941 cgctgctcgg tcaccgcgcg cgtgggtcat cggccatcac cgcagcactc ggtgggcgca
    21001 gttcggtccg tttctcgagt tcgccgtgcc gggttggcac acgtggcctc gagagcttgc
    21061 gtttcgccat cgcgatgttg cgctaatcca acgacgtcgc cggacacgtc agaccccctg
    21121 agtattgtga cttttcgaag ccgttgaacc gctagtacgt tcgtcgcagg ttggctaggt
    21181 ccagcagtcg gtcgaggtca agtccatgga cgtggtgtat gacgtcgtcg tgtgattctt
    21241 cgttgtgatg gttcaggcgg tcagggccgc gggggtggtc tcctgttctg ttgggttgtt
    21301 gccgacggtg cgtgatttgc tcaggatgtc gagtcctagg tagcgccggg attcggccca
    21361 ttcgtcgtgt tgttcggcca gtacggctcc aacgaggcgg atcagggcgt tgcggtcggg
    21421 gaagatgccg acgacgtcgg tgcggcgtcg gatttccttg ttgagccgtt cctgggggtt
    21481 gttggaccag atctggcgcc agatctgttt ggggaacgcg gtgaacgcca gcaggtcagc
    21541 ccgtgcgtct tcgaggtgct cggcaacctt gggtagcttg tcggccagcg catcaacgat
    21601 gcgatcgtat tgcgcggcaa cagattcagc atcgggttgg tcgaacaccg aatgcaacag
    21661 ggtgcgcacc cacggccacg acgccttcgg ggtgatcgcc atcaaattcg tcgtgtagtg
    21721 ggtgcggcag cgctgccagg ccgccccggg cagtgtggcg ccgatcgcgg ccaccagccc
    21781 ggcgtgggcg tcgctggtga ccagcttgac cccggtcagt ccgcgggcgg tcagcgaccg
    21841 caggaaggtc agccagccgg ccccgtcctc ggcggtggtg acgtcgatcc ccaggatttc
    21901 gcggtagccc tcggcgttga cgccgaccgc gatcagggcg tgcacgttga ccacccggcc
    21961 gccttcacgc accttgagca ccagggcgtc agcggccatg aacgtgtacg ggccggcatc
    22021 cagcggccgg gtccggaagg attccaccgt ggcgtcgagt tccttggcca tcacgctgac
    22081 ttgggatttg gacaggctgg tgatgcctag ggtctcgacg agtttgtcca tccgccgtgt
    22141 ggacaccccc agcaggtagc aggtggccac cacggtcgtc agggcccgct cggcgcgttt
    22201 gcggcgttcc agcagccagt ccgggaagta ggagccatgc cgtagcttgg ggatcgcgag
    22261 atccaatgtg cctgtcctgg tatcgaattc gcggcgccgg tagccgttgc gttgattggt
    22321 ccgctcggtg ctgcgttgcc cgtatcccgc accgcacagc gcgtcggctt ccgcacccat
    22381 caaggtgtgg atgaacgtgg acagcagctc gcgcaacacg tcggggtggg tagtggtgag
    22441 tcgttcggcc aacacctggg gccagtcgat atcgtgggca gtggtcatcg cgttgattcc
    22501 tttgctcgag tgactttgac gggtctctcg aagaatcacg cgatgacctt caatcattcg
    22561 gctacgacac gccggtaccg ctgatcaggt ccgactcata caccactctg ctggacgcaa
    22621 cctcggtcga acgctgacct cggtgtcgaa atgtcaacga tggccccatc ggagccgccg
    22681 ccacaacaat cacgatgtcg agatgcagcg aagattgaca agactcgcct ggctactttg
    22741 acaaaacttc ggcgtgtcac cggggcttgt taatcttcgt tgcagaagtc gcgctcatgg
    22801 tgcctcgtta gtcgggctcg gctaacggtc gaaacgccgt cctggcgccg acgcagggac
    22861 ggttcacgcc gccgagtcag ggcgcggcag gtcgtcgatg tcgggtagcg gtcgctggca
    22921 gatttcgcgg gcttcgtcgg cggtcatgcc gaacaagcgc agcacgtctt cggtgacctt
    22981 gtcggtggct tccccgtcgt cacgttcagg ttcgtcttgc aggagctggc ccagaccaaa
    23041 tagcgcgccc gccgcgacgg ccaacgcgag tttggggtcg tcgattcgga atcgccctgc
    23101 ggccgcggct gactcgatgt ctagcagtgc gcgtggcgct aggccacgat ccgaggacat
    23161 caggtgcagc ccgccgccga gaagtatctg gctctcacca ccttatcgaa cgaacgagtt
    23221 cgtttggtta cgatctacct gtgagaaaca caaccgagct gcggggcgaa atcttggcgg
    23281 ccgcgcgagc cgagttcgcc cagtacgggt tggccggggg ccaccgtgcc cgtcacaagc
    23341 ggactactcg tcacaccgac cagttgaggg caaccaaata gccgatagcg acgaccccga
    23401 gcgcggcggg gggcgctgcc ttgacgacgg catctacgtt ttgcaggtgt gtgccgactg
    23461 ctgcacccat cagcatgacc aggccccccg ccgccagcac acccaacggc gctgcgctga
    23521 agagcgagag cgcaagtccg gcggcggcgg caacctccaa ggcgccgatc atgcggtcag
    23581 cccgtatcga gtagtggatt tcggcggcca gatcgcgcat cggtttgacc gccgctacct
    23641 tcaggaggcc aagccctgcg aacacgaccg ccagcaggag cgctatgaca ttggcggcgt
    23701 tgataattgg catctcactc cgtatccggg gcattccagg cgatcgacgc cgagctccag
    23761 tgcgtcagct cccccgcctc ttcgttgacg tgtaagtggc gtggcggtgt cgcgaacgtc
    23821 gcataagggc ggcagcggat caccactcgg ttctcccgct cggccatctc gttgacgaaa
    23881 ggccgcgcct cttctccggg gtgctgtccc gacatgcgct cacctacggc catcatcaca
    23941 tcgaccacta ggtcgcggtc gcggtcgatg acggcgtcgg catacacctg caagaaggtg
    24001 aacggccagc gctcatccaa gacgcacagg ctcaccttgc cgtcacgctc caccacgcgg
    24061 gcctttccgc ggtcagccat cgtcgacacc aacagctcgc cgtcatcggt ggggatgtag
    24121 tagacgatcg tcattgccgg accgtcctgc cgacggcgat agccgaacac acacgtgcga
    24181 tgcgtgcgga cgaattcgcg ccgctccgag ggcagcatat ctcgatcggt gggtgcggtg
    24241 aatggctcgg aagccaatgg taacagtgcc acggtgtcct ctcgatcgct tggcgcgcag
    24301 ggcattacgc gatccgactt catcgcgttg cggccaaact aatttcggag acagtgttac
    24361 cggaataatt ccgagtgtca agctccgcag gaagacgttg acactcagcg gatctgactg
    24421 agtcccttga tgagcagatc cagcccgaag gaaaactcga cgtccagcgg cacaggcatg
    24481 gcgtcggcaa ctgccaaggt ggcccgaaat tgtgatgggt cgacagtgtg aaatgcctcg
    24541 gcgtcgtacg ccgcatgcgc ctcggggttt ccagagaatt ggatggcgaa cccgagcacg
    24601 tagcgcgaca gcgtggcgta gatgcgggcg gcaaggtcag gggcgaagcc gctgtcgagg
    24661 agcacggaca ggcagatttc gcggatcgcc atcgcgttgg gaccgaaggg agtgtgatcg
    24721 agcatcaggc gcgtgacgtt gggatggcgg ttcagcgccg catacatggt gcggacgaca
    24781 gattcacacg agcgctgcca gctctggccg gccagctcgg ccgcatccag gtgtacttcc
    24841 ccgtacacgt ggtccacaac gtgggcgacc aattctgagc ggttggcgaa atggcgatag
    24901 agcgtggccg taccggagtt cagctgctgg gccagcgtgc gcatcgacag cgcgtcggca
    24961 ccctgggcgt cgaccagctc gagggcggcg gcgactatgc ggcccggcgg catggccgga
    25021 cgaccccgag agcggcgccc cgacgcttgc tcggcgccgt ctctggcggt gttcacgctg
    25081 ctcagtatgc ccatcattga caccgatggc aatatcggta acactgtagc cattatggtt
    25141 ctacattccg ccaccgattc gcagttcaca gaggtcgacg gcctgagact gcactacaaa
    25201 cgtgccgggc gcggtcctgc agtgctgctg ctgcacggca ccacctcctc gctggagcac
    25261 ttcgaccgcg ccgcggcaat cctgcaaaag tccttcgacg tcatccgccc cgacctgccc
    25321 ggcttcgggc tcacagggcc gcggccccac ggcgactacc gggtgcggac ctatgccggc
    25381 accatggtcc agttcatgaa gaagttgaac attccgcgct acgcggtggc cggaaactcg
    25441 ttgggcggca acatcgcctg gaatctggcg ctcgatcacc cggaacgttt acgcgccctg
    25501 gtcttggtca acgccaccgg ctacccggag aagtcgctgc cggtcggcat gcggttggcc
    25561 cgcaatcccg ttgtgcggcc gctgatgcgc gcatggatgc cgcgacgcat ggtcgagcgc
    25621 ggcctacaac aggccgtggg accgcactcc accatcgtcg acgacgcgat ggtcacccgg
    25681 gtgcaccacc tgtggaacca accgggaaac cgttcggcgt tcgtcgattt cgtcaacacc
    25741 gaccagcccg accgcagcgc cgaaatcacc cgcataactg tgcctaccct ggtgctacgc
    25801 agcgccggca tcgacggcca acatttcgcc agggatattc ccggcagccg cgaagccatc
    25861 caccccgacg gcggacacct actgcccgaa gaagatcccg tctggtttgc cgatcagctc
    25921 accacatttc tgaacggcct cactgatcag gacgtctcat gaagtacttc accgttacta
    25981 gtgagaaccg ccaactcgac gacatcacgc gcggtcggct gcggggcagc ttcatcgaac
    26041 tcagcgacgg tgtcacgcat tacgaactgc gcggccccga ggaaggcgtg ctcgtcgttc
    26101 tcgtgccggg attgactatc ccgctgttct attgggacgc cttcgccgat gaactacacg
    26161 cccgcggcct gcgcacgctg gcattcagcg cctacgggcg cggctactcc gaccgggtgc
    26221 ccagccgtta tgacgacgcg ttgttcggcc gtcaactgcg cgagatcatc cccgcactgg
    26281 acttgcccgc gcgccaccac atcgtcggca cgtcgatggg cgccttgatc gccatgggct
    26341 atgtggcaga gcactccgag tccgtggcga cgctgacatt ggtgggaccg gccgggctat
    26401 ccgatgccac cgcgcgtcag cagcgactgt tgcgcagcga tgtgatcgcc acagtcgcag
    26461 ccaaactgtt cgggcggcgc attctgttgg gacacctcgg gcacaacgtc gccgacccga
    26521 acctggcacc gcagctgacg gccatgatcg ccgacaccta cgtctaccac ggctcgatgt
    26581 tcgcgttctt ctcgacgctg cagaactttc cgctctccgg acgaaagccg ttgtaccgcg
    26641 gcatcggtga ccgcagaatc cccacgatgc tgatgtgggg caccgaagat aaggtcaccc
    26701 cgatctcgga catggacacc gcctgcacgc tgctgcgccc cgccgaaacc cacatcatcg
    26761 actgcggtca catggcgccc tatgagcggc ccgtcgaggt cgccgaacat ctggcggctt
    26821 tcgccgccgc atacaccgat cggatcaccc catgacgaca cccatcctcg atgtcctcat
    26881 cgtcggcgcc ggccccgtgg gcgccgcgct ggccatcgac ctgacccgac gcggactgac
    26941 cgtgcgcatc atcgacaagg ctgatcacgc ctttgacggc tcccgcgcca aaggcattca
    27001 gccccgcacc ctcgaagtgc tcgaggacct cggcgccctc gaggaaatac tggccggcgg
    27061 atccacctat ccgctgatgg gcattcacct cggacctttc accgtgccgt ggcggatgat
    27121 gcgtagccga aaggccggcg acgacgtgcc gttcccagac acgtggctga ttccgcagtt
    27181 ccgcaccgac ggcgcgctgc acaaccgcct acgtgaactg ggggtcgcgg tcgaattcca
    27241 ctgtgagata gccgaactga cacaaacacc tgacgcagtg gccacctcgg tgacgaccag
    27301 ggacggcgtc gagacgatca ccgctcgctt catcgtcggt gcagacgggg gcggcagcgt
    27361 cgtccgcaag caggtcgaca tcgatttcgt cgggtcgacc gacgaggccg accgtgtcct
    27421 catcgtcgac gccgccgtgg acggcggcct gtcgcgtgac cgctggcacg tgtggcctgc
    27481 gcttcggggc cgcttcgtcg gagcgtgccc gctgccccac agtgaccaat tccaatggat
    27541 gatccgactg gcccccgacg aatcaccacc aaccgacgaa gcgcaaatca ccgaggtcat
    27601 tcgtgcgcgc accggcaaca agcggctggc cgtgcacgac atcgcgtgga agtcggtgtt
    27661 ccgccccaac attcggctgg cagagagcta ccggcgcggg cgggttttca tcgcaggcga
    27721 tgccgcacac gtgcatcctc ccgcgggcgc ccaagggctg aacaccggca tccaggacgc
    27781 ctacaacctc ggctggaaac tcgcccaggt cctcgccggc gccccagctg aactgctcga
    27841 aacctacgag acagaacgcc aacccatcgc ggcgggggtg ctgggcctgt ccaccaagaa
    27901 gtacagcgga ctgggaaaaa tggacccctc cagcatccgg cggggtgaag acgaacagca
    27961 actgtc
//
LOCUS       DASRJA010000571         9736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2465529,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000571 DASRJA010000000
VERSION     DASRJA010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2465529"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1033
                     /gene="tatC"
                     /locus_tag="VFC01_25290"
     CDS             83..1033
                     /gene="tatC"
                     /locus_tag="VFC01_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZN82964.1"
                     /translation="MQTPGILKKLDPRRRRSRVNPDGTMSLVEHLTELRTRLLIALAA
                     VVLTTIVGFFWYTHSFFGFHSLGEWLRGPYCSLPASARATIAPDGECRLLATAPFDQF
                     MLRLKVALTAGIILACPVWLYQFWAFVTPGLYKKERRFASAFVAVGAVLFVTGAVLAY
                     VVLAKALHFLLTVGSDVQVTALSGDQYFGFLINLLLVFGISFEFPLLIIMLNLVGVLT
                     YQRLKAWRRGLIFGLFVFAAFATPGSDPFSMLALALALSLLLEVSIQIARIHDKRKAR
                     RAALDEVPDEQAAPIGSAEPIEPPTAVPTPGRLVVDDDAT"
     gene            1017..3758
                     /locus_tag="VFC01_25295"
     CDS             1017..3758
                     /locus_tag="VFC01_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HZN82965.1"
                     /translation="MTTPLSGQLAGFAEQLSFKLDDFQQRACQAMENGHGVLVCAPTG
                     AGKTVMGEFAVHLALAAGRKCFYTTPIKALSNQKHNDLVARYGPERIGLLTGDQSING
                     DADVVVMTTEVLRNMLYANSPALYGLSYVVMDEVHFLADRMRGAVWEEVILHLPDDVR
                     VVSLSATVSNAEEFGGWIQTVRGDTTVVVDEHRPVPLWQHVMVGKRLFDLFDYRASKT
                     KSGRELLVDPELLRHIANRREADRLADWQPRGRGRGRPSIYRPPSRPDVISTLDREGL
                     LPAITFVFSRVGCDAAVKQCLRSSLRLTSDEERVRIAEIVDRRTADLNEADLVVLDYH
                     EWREGLLRGLAAHHAGMLPTFRHTVEELFTAGLVKAVFATETLALGINMPARTVLLEK
                     LIKFNGEQHMPLTPGEYTQLTGRAGRRGIDVEGHAVVLWHPDVDPAEVAGLASTRTFP
                     LRSSFAPTYNMTINLVNQMGPTQAHKLLESSFAQYQADRSVVGLVRAVERGERILDEI
                     AGELGGHDAPILDYVRLRLQISERERAQSRASRLQRRRAANDALAALRRGDIITITHG
                     RRGGLAVVLDADRDSDDPRPLVLTEHRWAGRISSADYSGTSETLGTMSLPKRVEHRNP
                     RMRRDLASALRSAAAGLDVPSAKGKRSGPPKERDVDPELAGLREQLRRHAAHDLPDRE
                     AVARLAERYLRIARDNEQIRQKIAAATNSLARTFDRIVVLLTERGFIDNRDGDPKVTD
                     DGRLLARIYSESDLLVAECLRSGTWEGLEAAELAAVVSSVLYESRGDAPGAPGAPDVP
                     TGKLRRALNQTRRLWTELRADEQRHRITQSREPEDGFVAAIYRWATTGDLTTALAASD
                     ASGTGTPLSAGDFVRWCRQVLDLLDQVRNAAPTPALRTTAKRAINDIRRGVVAVDAG"
     gene            3796..4614
                     /locus_tag="VFC01_25300"
     CDS             3796..4614
                     /locus_tag="VFC01_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HZN82966.1"
                     /translation="MSGPQGSDPTQPWPGQQPEQGKDQPSSDASANPPWQPPTSGAEQ
                     QPTQAAPAWQQPPAYDPQQQQYPQYQQPSAPAYQPPQQYPPTEQFGQQPSEYSPQAYP
                     GQYGQPGQYGQPGQYGQPGQQYAPPGQQYGQQPGQYGQQPDQYGQYPQYTAPGAEEGS
                     KRSLAVVGGVIGLLAAIIVAVVLVMGFWKPGFFVTTKLDVNAAQTGVQQILTDEANGY
                     GAKNVKDVKCNGGTSPTVKKGDTFNCEVSIDGTKRQVTVTFQDDNGTYEVGRPK"
     gene            complement(4622..5572)
                     /locus_tag="VFC01_25305"
     CDS             complement(4622..5572)
                     /locus_tag="VFC01_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HZN82967.1"
                     /translation="MSLVLLDGASMWFRSFFGVPSSITSPDGRPVNAVRGFLDAVATV
                     ITREQPSRLVVCRDDDWRPQWRVDRIPSYKAHRVAVEEPAGQPDIEEVPDDLTPQVDM
                     ILEILDAFGIPTAGAPECEADDVLGTLAFRERRDPVIVVSGDRDLLQLVCDEPVEVRV
                     LYLGRGLAKATKFGPAEVAETYGVPVDRAGPAYAELALLRGDPSDGLPGVPGVGEKTA
                     ATLLAQHGSLEDILAAAHDPKSKMSKAYRTKLLAATDYIEAAGPVVRVATDADVNFST
                     PSDTLPLAAEHPRKVVKLAEQYGVTSSIGRLQKALDALPG"
     gene            5571..6095
                     /locus_tag="VFC01_25310"
     CDS             5571..6095
                     /locus_tag="VFC01_25310"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN82968.1"
                     /translation="MRAILPGVVGAIRGYPVGMPQVQVFAEVRRSAESMWRELGSFQD
                     IAQWHPMVKAAEGEGEEPGATRTLETQDGRRWVERLTERDPAQRFYRYEVTSTELPIA
                     DFRGELRIREGGPQKCTVIWTSQFAVTTDEEKAVSDSVREFFRAGARSIEKRFAVKPV
                     AALRRRVRRVQRRR"
     gene            6140..8764
                     /locus_tag="VFC01_25315"
     CDS             6140..8764
                     /locus_tag="VFC01_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZN82969.1"
                     /translation="MRLSWPLTGRSDEMRAIEAAILAPDVSGIVVRGPAGVGKSRIAR
                     EALSAAVSRGWEGRWAIGTSAARTLPLGAFTAWAQSGVTDTVQLVRGVIESLTAAPPG
                     TSVLVGVDDVHLLDDLSAFAVQHIVQRGAAKVLLTVRDGEPIPAAVQEIWAGGQFDRL
                     DLRPLSFEQTTTLLSASLGGSVEPDAAQRLWTLTDGNVLYLRNIVEQEVADGRLEMQP
                     GYWRWTGDPIVAPGLVELIESRIGDLPAPVADVIDALAVGEPLELAALTRITDPAAVE
                     EADTRGLITLEPTADGVEVRVAHPLYGEVRRKRAASTRLRRLRGLVAAELAASDDRDD
                     IRLVVRRATLSLDSDLRPDSELLIRAAHGAVWLADLPLADRLAGAAIRAGAGPEANFI
                     RAHALSWLSRGQEADTVLAATDSRWLTDADHAKSAFLRASNMLWVLADPAGAKELIEA
                     ASHTTPLAARSYIDAFLTVYWFAMDQPGLAIQASKDLAFDDLPAVVDAEIAWARVAIS
                     AEAGRTTEAAAIADAGYVVATRSLDAPQMRFNIADAHVSALLLSGQVGDAAEVAQGMR
                     DQAADLPGTARLLGPAVLGRAALGAGQLDTACLLLEQAAEALSAAGYAIGWGFRYHVP
                     YSTALAIRGSTRKAAAVLAALDKLGRPFRSLDYERSLARAWLAANQGAVSEAITVLVS
                     AAERARANGQFAAEVICLQTATQFGHRLGGPRLRELEGIAEGPRVGLAVRLTEALHDG
                     NAAELTAVSMDFEGMGDVVAAIDAAAHAAIEHRRRGQRGSALVCSTRADGLALQCGGA
                     STPALRQASERLPLTDREREVAMLTADGLSSPAIAERLTLSVRTVESHLYRAMLKTGT
                     ASREELANLLRRHTPDTP"
     gene            9152..9466
                     /locus_tag="VFC01_25320"
     CDS             9152..9466
                     /locus_tag="VFC01_25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82970.1"
                     /translation="MKHLIRTSLTAAAVAAAALGLPAAANAAPAGPGSVDATINELRA
                     QGFDVVVNRIGTAPQEQCTLSAVRPGQTFSRSDTGSGLPGSAFDIVTTVTGKTVYVDI
                     AC"
     gene            9542..>9736
                     /locus_tag="VFC01_25325"
     CDS             9542..>9736
                     /locus_tag="VFC01_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN82971.1"
                     /translation="MAFVVDRVDHVVLNCRDVDITVDWYVRVLGMRREVFGDNRIALA
                     FGNQKINVRPTGTPNWETGAV"
BASE COUNT         1704 a         3173 c         3231 g         1628 t
ORIGIN      
        1 aggccgagca gccgactgac caacgcccgg cctgacccac gccgacccca ctggccgcac
       61 gatgaggcgg cgtagatagc ccgtgcagac ccccggaatc ctgaagaaac tcgatcctcg
      121 tcgacgtcgg tcgcgggtca atcccgacgg caccatgtcg ctggtcgagc atctgaccga
      181 actgcggacc cgcctgctga tcgccctggc cgccgtcgtg ctgacgacaa tcgtcgggtt
      241 cttctggtac acccacagct tcttcggatt ccacagcctc ggtgagtggc tgcgcgggcc
      301 gtactgttcg ctgccggcat ccgcacgcgc gaccatcgcg cccgacggcg aatgccgctt
      361 gttggccacg gcgccgtttg accagttcat gcttcggctc aaggtggcgt tgacggccgg
      421 gatcatcctg gcctgcccgg tgtggctgta tcagttctgg gcgttcgtca cgcccggcct
      481 gtacaagaaa gaacggcggt tcgcgagcgc gttcgtggcg gtcggcgcgg tgctgttcgt
      541 caccggagcc gtgctggcct acgttgtgct ggcgaaagcg ctgcacttcc tgctgaccgt
      601 cggtagcgac gtacaggtca ccgccctgtc gggtgatcaa tacttcggtt tcctgatcaa
      661 cctgctgctc gtgttcggca tcagcttcga attcccgctg ctgatcatca tgctcaacct
      721 cgtcggggtg ctcacttacc agcggctaaa ggcctggcgg cgcggactga tcttcgggct
      781 atttgtgttc gccgcgtttg cgacaccggg ttccgaccca ttctccatgt tggcgttggc
      841 gctggcactg tcgctgctgc tcgaggtttc cattcaaatc gcccggattc acgacaagcg
      901 caaggcccgc cgcgccgcgc tcgacgaggt acccgacgag caggccgcac cgatcggcag
      961 cgccgaaccg atcgaaccgc cgaccgctgt gccgacaccc ggccggctcg tagtcgatga
     1021 cgacgccact tagcggtcaa ctggccggct tcgccgagca gctgtccttc aagctggacg
     1081 actttcaaca acgcgcctgc caagcaatgg agaacggcca cggcgtgctg gtgtgcgcac
     1141 cgaccggcgc cggcaagacc gtcatgggcg aattcgccgt ccacctcgcg ctggcggcgg
     1201 gccgaaagtg cttctacacc accccgatca aggcgctgag caaccagaag cacaacgatt
     1261 tggtggccag atacggccca gagcgcatcg ggctgctcac cggcgaccag tcgatcaacg
     1321 gcgacgctga tgtcgtggtg atgaccaccg aggtgcttcg caacatgctg tatgcgaatt
     1381 cgcctgcgct gtacggcctt tcatatgtcg tcatggacga ggtgcacttt ctggccgacc
     1441 gcatgcgcgg tgcggtgtgg gaggaagtga tccttcatct gcccgacgac gtgcgggtgg
     1501 tgagcctgtc ggcgacggtg agcaatgccg aggaattcgg cggctggatc caaacagtgc
     1561 gcggcgacac caccgtcgtc gtcgacgaac accgcccagt cccgttgtgg cagcacgtga
     1621 tggtgggcaa gcgtctcttc gacctcttcg actaccgcgc ctccaagacg aagtccggcc
     1681 gtgaactgct cgtcgacccc gaattgctgc gtcacatcgc gaaccgccgc gaggccgacc
     1741 gactggccga ctggcagccg cgcggccggg gccgtggccg tccgagcatc taccgaccac
     1801 cgagccggcc cgacgtgatc agcaccctgg accgtgaagg tcttttgccc gcaatcactt
     1861 tcgtgttctc acgcgtcggc tgcgacgccg cggtcaagca atgcctgcgc tcgtcgctgc
     1921 gactcacctc cgacgaggaa cgcgttcgca tcgccgaaat cgtcgaccgc cgcaccgcgg
     1981 atctcaacga ggccgatctg gtcgtgctcg actatcacga gtggcgtgaa gggctgctgc
     2041 gcggtttggc cgctcaccac gcgggaatgc tgccgacctt ccgtcacact gtcgaagagt
     2101 tgtttactgc aggactggta aaggccgtat tcgccacgga gacactggct ttgggcatca
     2161 acatgcctgc cagaacagtg ctgctggaaa agttgatcaa attcaacggt gagcagcaca
     2221 tgccgttgac gccgggggag tacacccaac tgacgggtcg ggccgggcga cgcggcatcg
     2281 acgttgaggg ccacgcagtg gtgctgtggc atcccgacgt cgatccggcc gaggtcgccg
     2341 gcctggcgtc gacccgcacg ttcccgttgc gcagctcgtt cgcgcccacg tacaacatga
     2401 ccatcaatct ggtgaaccag atgggtccga cgcaagcgca caagttgctg gaaagctcct
     2461 tcgcgcaata ccaggcggac cgctcagtgg tcgggttggt gcgggccgtc gagcgcggcg
     2521 aacggatact cgacgagata gcaggcgaac tcggcggaca cgacgcgccg atcctggact
     2581 acgtgcggct gcgcttgcag atctccgaac gcgaacgggc ccaatctcgg gcatcgcggc
     2641 tgcaacgacg cagggccgcc aacgatgcgc tggccgcgct gcgccgcggc gacatcatca
     2701 ctatcacgca cggtcgccgc ggaggcttgg ccgtggtgct cgacgccgac cgcgacagcg
     2761 acgatccgcg accgctcgtg ttgaccgaac accgttgggc ggggcgtatt tcgtcggccg
     2821 actattccgg cacatcggag acgctcggca caatgtcgct gcctaaacgt gtcgaacacc
     2881 gcaatccgcg gatgcggcgc gatctcgcgt cggcgctgcg atccgcggcg gccggactgg
     2941 atgtcccgtc ggccaagggc aagcgcagcg ggccgccgaa ggagcgcgac gtcgaccccg
     3001 aattggccgg gctgcgtgaa caactgcgcc gacatgccgc gcacgacctg ccggaccgcg
     3061 aagcggtggc ccgactggcc gagcgctatc tgcggatcgc gcgggacaac gaacagatcc
     3121 ggcagaagat cgccgcggcg acgaattcgt tggcacgcac attcgatcga atcgtcgtgc
     3181 tgctgaccga gcgcgggttc atcgacaaca gggacgggga ccccaaggtc accgacgacg
     3241 gccggctgct ggcccggatc tacagcgaga gcgacctgtt ggtcgccgag tgtctgcgca
     3301 gcggcacctg ggagggtctg gaggccgccg aattggcggc ggtggtgtcg tcggtgctct
     3361 acgagtcacg cggtgacgca cccggcgcgc ccggcgcgcc cgacgtgccg actggcaagt
     3421 tgcgtcgtgc cctgaaccag acccgccggc tgtggaccga gcttcgtgcc gacgagcagc
     3481 gtcaccgcat cacccagagc cgcgagcccg aggacggttt cgtcgcggcc atctaccggt
     3541 gggccaccac cggcgacctg accaccgcac tcgcggcgtc cgacgcgtcg ggcaccggga
     3601 caccgttgtc ggccggtgat ttcgtgcggt ggtgccgtca ggttctcgac ctgctcgacc
     3661 aggttcgcaa cgctgcgccc acgcccgcac tgcgcaccac cgcgaaacgc gctatcaacg
     3721 acattcgacg cggcgtcgtg gctgttgatg ccgggtagtg tgtttgcagc agcgcgacca
     3781 tgagcaagga gagacatgag cggaccgcag ggatctgatc cgacgcagcc gtggcccggg
     3841 cagcaacccg agcagggcaa ggatcagccg tcaagcgacg cctcggccaa cccgccgtgg
     3901 cagccgccga cctccggggc cgagcaacaa ccgacccaag cggctccggc gtggcagcag
     3961 ccgccggcat acgacccgca acagcagcag tatccgcagt accaacagcc gtcggcaccc
     4021 gcctaccagc cgccgcagca atacccgccg accgagcagt tcggccagca gcccagcgag
     4081 tacagccctc aggcgtaccc cggtcagtac ggccagcccg gccagtacgg gcaacccggt
     4141 cagtacgggc agcccggcca gcaatacgcg ccgcccggcc agcagtatgg ccagcagccg
     4201 ggtcagtacg gccagcagcc ggaccagtac gggcagtacc cgcagtacac cgcgccgggc
     4261 gctgaggagg gctcgaagcg atcgctggct gtcgtcggcg gcgtgatcgg cctgctggcc
     4321 gcgatcatcg tggccgtggt gctggtgatg ggcttctgga agccgggctt tttcgttacg
     4381 acgaagctcg acgtcaacgc cgcgcagacg ggtgtgcagc agatcctgac cgacgaggcc
     4441 aacggctacg gcgccaagaa cgtcaaggac gtcaagtgca acggcggcac gagccccacg
     4501 gtgaagaagg gcgacacctt caactgcgaa gtcagcattg acgggaccaa gcgccaggtg
     4561 acggtgacgt tccaggacga caacggcact tacgaggtgg gtcggcccaa gtagcccggc
     4621 gctaccccgg tagcgcgtcg agcgctttct gcaaccggcc gatggacgac gtaacgccgt
     4681 actgttcggc aagtttgacg accttgcggg ggtgctcggc cgccagcggc agcgtgtctg
     4741 acggtgtgga gaagttgacg tcggcatcgg tggccacacg cactaccggc ccggcggcct
     4801 cgatgtagtc ggtggccgcg agcagtttgg tccgatacgc cttggacatc ttcgatttgg
     4861 ggtcgtgcgc ggcggccagg atgtcctcga gtgagccgtg ctgtgcgagc agcgtcgcgg
     4921 ccgtcttctc cccaacaccg gggacaccgg gcagcccgtc agagggatcg ccgcgcagca
     4981 aggccaattc cgcgtatgcg ggtccggcgc ggtcgacggg cacgccatag gtctcggcga
     5041 cttctgccgg cccgaacttc gtcgccttcg ccagcccacg gcccagatag agcaccctga
     5101 cctcgaccgg ctcgtcgcac accagttgca gcagatcccg gtcgccgctg acgacgatga
     5161 ccgggtcccg gcgctctctg aacgccaagg tgccgagaac atcgtcggcc tcacattccg
     5221 gcgccccggc cgtcggaatg ccgaacgcgt ccaaaatctc gaggatcatg tcgacctgag
     5281 gggtcagatc gtcgggcacc tcttcgatgt cgggctggcc ggcgggttcc tcgaccgcga
     5341 cgcggtgcgc cttgtaggac gggatgcggt ctacccgcca ttgcggacgc cagtcgtcgt
     5401 cgcggcacac caccagccgc gacggctgct cccgcgtaat caccgtcgcg acggcgtcca
     5461 ggaagccgcg cacggcgttg acgggccgcc cgtccggtga ggtgatcgat gagggcacac
     5521 cgaaaaacga gcgaaaccac atgctggcgc cgtcgagcag cactaaggac atgcgggcca
     5581 tcctgccagg ggtggtgggt gcgatccgcg ggtatccggt cggcatgcca caggtccagg
     5641 tgttcgccga agtccgtcgc agtgccgagt cgatgtggcg cgaactcggg tcgtttcagg
     5701 atattgcgca gtggcatccg atggtcaagg cggcagaagg cgagggcgaa gagcccggcg
     5761 cgacgcggac tttggagacc caagacggcc gacgatgggt cgagcggttg accgagcgtg
     5821 accccgcgca acggttttac cggtacgagg tcacctcgac cgagctgccc atcgccgact
     5881 ttcgcggcga gttgcggatc cgcgaaggcg gaccacagaa atgcaccgtg atctggacgt
     5941 cccagttcgc ggtcaccacg gatgaggaaa aggccgttag tgactcggtg cgtgagtttt
     6001 tccgcgccgg agctcgctcg atcgagaagc gatttgcggt caagccggtc gccgccctgc
     6061 gccggcgcgt acggagagtg caacgccggc ggtagtcgtt cgctctatgt agtgctctcg
     6121 gccgtcttag tctcgtgggg tgcgcttgtc gtggccgtta actggccgct cggacgagat
     6181 gcgtgctatc gaggccgcca tcttggctcc tgacgtctcc ggaatcgtgg ttcgcgggcc
     6241 ggcaggcgtg ggaaagagcc gaattgcccg tgaggcattg tcggccgcgg tgtcgcgagg
     6301 atgggaaggc cgatgggcga tcggcacatc cgccgcgagg acgcttccac ttggcgcttt
     6361 caccgcatgg gcgcagtccg gtgtcaccga caccgttcaa ctggtgcgcg gcgtgatcga
     6421 gtcgctgacg gccgcaccgc cgggaacttc ggtgctggtc ggcgtggacg acgtgcatct
     6481 gctggatgat ctttcggcgt tcgctgtgca gcatatcgtg cagcgcggcg cagcgaaggt
     6541 gttgctcacc gtccgtgatg gtgaaccgat tcctgcagcg gtgcaggaga tttgggcggg
     6601 tggccagttt gatcggctcg atctaaggcc tctgtcgttc gagcagacga ccacactgtt
     6661 gtcggcgagt ttgggcggat cggtggaacc ggatgccgct cagcggctct ggacgctgac
     6721 tgatggcaat gttttgtatc tgcgcaacat cgtcgagcag gaggtcgccg acggccggct
     6781 cgaaatgcag cccggatact ggcgatggac cggtgatccg atcgtggcgc ccggcctggt
     6841 tgagctgatc gaatcccgca tcggtgacct tcccgcaccg gttgccgatg tgatcgatgc
     6901 gctggccgtc ggcgagccgc tcgagctggc ggcgctaacg cgcatcactg atcctgcggc
     6961 ggtcgaggaa gccgacacgc gcggcctcat cacgcttgag cccacagcgg acggtgtaga
     7021 ggtgcgtgtg gcgcatccgc tttacggtga ggtgcgcaga aaacgggcgg catccacgcg
     7081 gttgcggcgg ctgcgcggac tcgtcgccgc cgaactcgcc gcatccgatg accgcgacga
     7141 catacggctc gtagtgcgcc gcgccacgtt gagtctcgac tccgatctga ggcccgactc
     7201 cgagctgctc attcgggccg cacatggcgc ggtgtggctg gcagacctgc cgctagcgga
     7261 tcgactcgcg ggagccgcga tccgcgccgg agcgggaccg gaggcgaact tcattcgtgc
     7321 gcatgcgctt tcgtggctca gccgtgggca ggaggccgac actgtgctcg ctgcgactga
     7381 cagcagatgg ttgacggacg ctgatcatgc caagtcggca ttcctccgcg ccagcaacat
     7441 gctttgggtg cttgccgacc ccgctggcgc gaaggagctc atcgaggcgg cgtcgcacac
     7501 cacaccactg gcggctcgca gctacatcga tgccttcctc acggtgtatt ggttcgccat
     7561 ggaccagccg ggtttggcga tacaagcgtc gaaagacctt gcgttcgatg acctgcccgc
     7621 cgtcgttgac gccgagatag cctgggcgcg cgtggctata tcggctgagg ctggacgcac
     7681 aacggaagcg gcggccattg cagacgcggg atacgttgtt gcgacgcgct ctttggacgc
     7741 accgcagatg aggttcaaca tcgctgatgc acatgtcagc gcactgttgc tgtccggtca
     7801 ggtcggagat gccgccgagg tggcacaggg gatgcgcgat caagcggcgg atctcccggg
     7861 gacggcgcga ttgctcggcc ccgcggtgtt gggtcgggcg gcacttggtg ccgggcagct
     7921 cgataccgcg tgcttgttgc tggagcaggc agcagaagcg ttgtcggccg cgggctatgc
     7981 aatcggctgg ggattccgct atcacgtccc gtactcgacc gccctcgcca tacggggctc
     8041 aactaggaag gcagccgcag tgcttgccgc gctcgacaag ctgggacgtc cttttcggtc
     8101 cctggactat gagcggagcc tggctcgcgc ctggctggcc gctaaccaag gcgccgtcag
     8161 cgaggccatc accgtcctgg tgtcagcggc cgaaagagcc agagccaatg ggcaattcgc
     8221 ggctgaagtg atctgcctgc aaaccgcaac gcagttcggg caccgtttgg gcgggccccg
     8281 gctgcgcgaa ctcgagggaa tagccgaagg gccgcgcgtt ggccttgccg tacggcttac
     8341 cgaggctctg catgatggca acgccgccga attgactgcg gtgtccatgg atttcgaggg
     8401 catgggcgac gttgtcgcgg ccatcgacgc ggctgcgcac gcggccatcg agcatcgccg
     8461 ccgaggacag cggggatcgg cattagtctg ttcgacacgt gcggatgggc tggctctaca
     8521 gtgtggcggc gccagcacgc cagcgcttcg tcaggcgagc gagcgcttac cgttgaccga
     8581 tcgcgaacgc gaggtcgcca tgttgactgc cgacggatta tcgagcccag ccattgccga
     8641 acgactcacg ctttcggtac gcaccgtcga aagccacctc taccgggcca tgttgaagac
     8701 cgggactgcc agccgcgaag aactcgccaa cctgctacgt cgccacacgc cggacacacc
     8761 gtaaccagcg tcgtccgaca tgcacgccgc atcggcgatg agacgagtac ctgattactg
     8821 aatcgctgta accgccaccg cgaccttcga acgcatcacg ctggcgaaac gggatgcgac
     8881 gcaacggcaa cggcgggccg ctatcacaag cgccactgac gggcattgat tgagccatgt
     8941 cgcgggcgcc agcaataccc gcaccgccaa cgccgcaacc aggacaacgt gagattccag
     9001 ccgcctggtc acggagcagg cggctgctcg agatcgcgtc taagtaatcc gcgagccgaa
     9061 ccacggcgag atttcagtaa cgcgctgcgc agctactccc gcccgacgcg atcgacgatt
     9121 tctgtcataa ggacacaaga aaggcaagac catgaagcat ctgatccgca cctccctcac
     9181 cgcggccgcc gtggccgcag cggcgcttgg cctgccggca gccgccaacg ccgctccggc
     9241 cggcccgggg tctgtggatg ccacaatcaa cgagctgcga gcgcaaggct tcgacgtcgt
     9301 ggtgaatcgc ataggcacag ctccgcaaga gcagtgcact ctgagcgcgg ttcgccccgg
     9361 ccagacattc tcgcgtagcg ataccgggtc cggtctaccc ggatccgcct tcgacattgt
     9421 caccacggtg acaggcaaga cggtctacgt cgacatcgct tgctgacgtc agactcgagg
     9481 ggcgcccaca acgggcgccc ccctttttgc cgaccggccc gatcttgaga taggaaaggg
     9541 catggccttc gttgttgacc gcgtcgacca cgtggtgctc aactgccgcg acgtcgacat
     9601 caccgtggac tggtacgtgc gggtgctcgg catgcggcgt gaagtcttcg gcgacaaccg
     9661 gatcgcgctg gcgttcggta accaaaagat caacgtgcgt ccgaccggca caccgaattg
     9721 ggagacgggt gccgtc
//
LOCUS       DASRJA010000572         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1006062,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000572 DASRJA010000000
VERSION     DASRJA010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006062"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VFC01_25330"
     CDS             <1..521
                     /locus_tag="VFC01_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZN82972.1"
                     /translation="ELASDTLVATVMSNMGLHLAMRSAGIDVRTTGVGDRYVLEELRA
                     GEYSLGGEQSGHIVMPALGTTGDGIVTGLRLMSRMAQTRSTLAALAEPMQTLPQVLIN
                     VQVADKATVADAPSVRSAVAEAEAQLGDSGRILLRPSGTEQVVRVMVEAADEDTARQV
                     AVRVAESVSDQR"
     gene            565..870
                     /locus_tag="VFC01_25335"
     CDS             565..870
                     /locus_tag="VFC01_25335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion target"
                     /protein_id="HZN82973.1"
                     /translation="MGQAEVARVDVAALHAVAREYDAVADIVEGLVRTHLIGLAFDGA
                     VAGRMHVARGDALRVVVDHVVDQLRQWSRASAEIGAALRASADRYAEADSSAASRVG"
     gene            873..2207
                     /locus_tag="VFC01_25340"
     CDS             873..2207
                     /locus_tag="VFC01_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82974.1"
                     /translation="MAEKFDIPSRLAEGRPAVDNIQTYVWACHMLGYANPDLTLHASQ
                     VSDWYGSEDALDLRALDADCAALEAAVAATEDALMRQDEQLAALSSAWQGGTADLSRE
                     FLRRHGEASAAAAAAVRTTADALATLRDNLWHAVDGKVAAAIAADDRAPGEWLAAAHT
                     VTTGAGDRAAASERIDQEVKPFVDNDIRAEWLTAMRSAMAAVMESYDAATAELSAEPD
                     AVFDVPGDLGPSWTPPPRDDEAATVPAAASSVAPPVAAAPPWGAPASAWAPAPAPPPV
                     APPPAPPVDPGTTAPAMSAPPSAPSLGGQPDVGSGLSGIGQQLADVFGSLLGSADDAL
                     AETPDIDEPELDEDVDDELDEDPEEEDEPVEPVADPEEEPAADQPVEAPAADETCEPA
                     EAPIDPPPPEEPAPTPAPVPPPPPEPAPLADPAAAAETPCEIAADALPQVGE"
     gene            2219..2371
                     /locus_tag="VFC01_25345"
     CDS             2219..2371
                     /locus_tag="VFC01_25345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82975.1"
                     /translation="MNAELAEELVTMAEKGRAVRAALSSEDPANIAKLRASKARRTQS
                     YPPDHP"
BASE COUNT          446 a          894 c          969 g          405 t
ORIGIN      
        1 gcgaactcgc ctccgacacg ctggtcgcca cggtgatgag caacatgggc ctgcacctgg
       61 cgatgcggtc ggccggcatc gacgtccgca ccaccggcgt cggcgaccgc tatgtgcttg
      121 aagagcttcg ggcgggcgaa tattcgctcg gtggtgagca atcggggcac atcgtgatgc
      181 cggcgctcgg cacgacggga gacggcattg tcaccgggtt gcggctgatg tcgaggatgg
      241 cgcagacgcg ctcgaccttg gcggcgctgg ccgagccgat gcagacactg ccccaggtgt
      301 tgatcaacgt gcaggtcgcc gacaaggcca ccgtggcgga cgcgccgtcg gtgcgctcgg
      361 cggtggccga ggccgaggct cagctcgggg acagcggccg aattctgttg cggccctccg
      421 gaaccgagca ggttgtccgc gtgatggtcg aggcggccga cgaggacacc gcgcgtcagg
      481 tggccgtccg agtcgctgaa tcggtcagcg accagcgctg aagttcgttg gaaccggcgg
      541 gggttcgccg gcgtcctagc gtgtatggga caagctgagg tcgcccgtgt cgacgtggcg
      601 gcgttgcacg ccgtcgcgcg tgagtacgac gcggtcgccg acatcgtcga gggcttggtg
      661 cgaacgcacc tgatcggcct ggcgttcgac ggagccgtcg cgggccgcat gcacgtcgcc
      721 cgcggtgatg ccctgcgggt cgtcgtcgat cacgtcgtgg accaactccg acagtggtcg
      781 agggcttccg ccgagatcgg tgccgcgtta cgcgcatcgg ctgaccgcta cgccgaggct
      841 gatagcagcg ccgccagccg agtggggtag cgatggccga aaagttcgac atcccatcgc
      901 gattggcgga ggggcgcccc gccgtcgaca acatccagac atacgtgtgg gcttgccaca
      961 tgctcggcta tgcgaacccg gatttgacgc tacacgcgtc acaggtgagc gactggtacg
     1021 gcagcgagga cgcgctcgat cttcgtgccc tggacgccga ttgcgccgcg ttggaggcag
     1081 cggtggcggc gacggaggac gcgttgatgc ggcaggacga gcaacttgcc gcgctatcgt
     1141 ccgcctggca gggcggcacc gcggacctgt cgcgtgagtt tctgcgccgg catggtgagg
     1201 catcggctgc ggccgcggcc gccgtgcgca cgacggcgga cgcgttggcc acgctgcggg
     1261 acaacctgtg gcatgccgtc gatggcaagg tggcggcggc gatcgctgca gatgaccgcg
     1321 caccgggcga gtggctggcc gccgcgcaca ccgtcacgac gggtgcgggc gatcgggccg
     1381 ccgctagtga acggatcgac caggaagtaa agccgttcgt agacaacgat attcgcgctg
     1441 agtggctcac ggcgatgcgt tcggcgatgg ccgcggtgat ggagtcgtac gacgctgcga
     1501 cggctgagct aagcgccgag cccgatgcgg tgttcgatgt gcccggtgat ctcggcccgt
     1561 cgtggacgcc gccaccgcgt gacgacgaag ccgccacggt tcccgcagcg gcgagttctg
     1621 tggcgccgcc cgtcgccgcg gcgccgccgt ggggtgcacc tgcatccgct tgggcaccag
     1681 cgcctgcgcc accaccagtg gcgccaccgc ccgcgccgcc ggtcgatccg ggcacaaccg
     1741 cacccgcaat gtcggcaccg ccgtcggcgc cgtcgctcgg tgggcagccg gatgtcggca
     1801 gcggtctgtc cgggattggc cagcagcttg ccgatgtgtt cggcagcctg ctgggatcgg
     1861 cggacgacgc cctggcggag acacccgata tcgatgagcc ggagctcgac gaggacgtcg
     1921 acgatgaact ggatgaggat ccggaagaag aggatgagcc ggtcgagccc gtggccgatc
     1981 cggaagaaga gcctgccgcc gaccaacctg tcgaggcccc tgcggccgac gaaacatgcg
     2041 aaccggctga agctccgatc gacccgccac cgcccgaaga gcccgcgccg acgccggccc
     2101 cggtgccgcc gccgccgccc gagcctgctc cgttggccga cccagcagcc gccgccgaaa
     2161 caccgtgtga gatcgcggcc gacgcactgc cgcaggtggg ggagtgatgg agcaaggctt
     2221 gaacgcggaa ctagctgaag aactggtgac aatggccgaa aaggggcgtg ccgtgcgcgc
     2281 ggctttgtcg agcgaagacc cggccaacat cgccaaactc cgcgccagca aggcgcggcg
     2341 cacacagtcc tacccaccgg accaccccta ggtccgtgaa gtcggtcagg cactcgatta
     2401 cgaaatgctg gcccagcacg ccgccaaggt agttgccgat tggccgaccc cagcacccga
     2461 acttctgcgg cgcgtacaac cttgcgacgc cgtggtgtag gggccaccgc ttaacaagca
     2521 ttgcaccgcg cttgaagggc cggctgcggt gcggaactcc aacaccaccc ttgatcccca
     2581 cggtggcggg ccgactcacg tttcggattc gtcgaactag gtcaggcgcc tactcgtgtt
     2641 cgtatgagga aggcaagttc ttacatacgc ccggacttgc cctctaggtc gccctgccac
     2701 cccgggcggc ggcg
//
LOCUS       DASRJA010000573         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_865790,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000573 DASRJA010000000
VERSION     DASRJA010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865790"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFC01_25350"
     CDS             complement(<1..440)
                     /locus_tag="VFC01_25350"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN82976.1"
                     /translation="MADVVHYDITDRVAVLTIDNPPVNALSPAVWEAIDQGVQRANAD
                     PAADAIVLMGAGTTFIAGADIKVFDLLKTPQDALTRSAGTHALLRRMEDSTKPLVAAI
                     HGNALGGGMEVAMSCHFRVAARDAKVGQPEVLLGIIPGAAGTQRL"
     gene            complement(467..937)
                     /locus_tag="VFC01_25355"
     CDS             complement(467..937)
                     /locus_tag="VFC01_25355"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN82977.1"
                     /translation="MTHVEARRRMSGLEFFQRMIAGEMPPPPMLDLLGIRIAEVEEGR
                     VVFTAVCEESFYNGTGVAHGGFAATMLDTALGCAINSAMPAGRRFTTLELKVNLTRPI
                     TKEAGLLRCEAKVVHVGGRTATSEGRIVDAAGKLYAHGTTTCIIVDAPRGTLEP"
     gene            complement(934..>2259)
                     /locus_tag="VFC01_25360"
     CDS             complement(934..>2259)
                     /locus_tag="VFC01_25360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN82978.1"
                     /translation="RVVVTLDLLAPLVQGVRSQTSIEHVIVTSLAEYSAAATPTPRID
                     GTLSLTELTRRQSVSASGPTAGSPGSDPGLTRVGPEDIAVLQYTGGTTGTPKGAMLTH
                     GNLWANVVQTENWMNPAYTLSGNERYLVVIPYFHIYAFAVCMLVGLRVGALQIIHPKY
                     DPEQVLAAIRDFRPTYFPAVPTVFVSLLNHPKVGEYGLDLIRLYNSGGAPCPVEVMEE
                     FERRIGRPLNEGYGLSETSPVTHSTPQLALRKFGTVGLPFPDTDMKIVDVETGTHELP
                     VGDTGELCIAGPQVMKGYWNKPEESAHVLRTHADGRIWFHTGDIARMDADGYTSIVQR
                     KKDMIIVDGYNVYPSDVESVLYAHPAVRLAAVIGIPDNYHGEIVKACIALKPGSSVTA
                     DEVIAHCRISLTDYKVPRVVEIRDTLPMSAVGKILYRVLRDEHAAGTGS"
BASE COUNT          357 a          728 c          762 g          412 t
ORIGIN      
        1 agccgctgcg tgccggcggc gccgggaatg atcccgagga gcacctccgg ctggccgacc
       61 ttcgcgtccc gcgcggcgac gcggaagtga cacgacatcg cgacttccat gccgccgccg
      121 agcgcgttgc cgtgaatcgc ggcgacgagt ggcttggtcg agtcctccat gcggcggaga
      181 agcgcgtggg tccccgccga ccgggtcagc gcgtcctgtg gcgtcttgag caggtcgaac
      241 accttgatgt ccgcgccggc gatgaacgtc gtgccggcgc ccatcaatac gatggcgtcg
      301 gccgccgggt cggcgttcgc gcgctggacg ccctggtcga tcgcttccca caccgcggga
      361 ctcagcgcgt tgaccggcgg gttgtcgatg gtcaggaccg cgacgcggtc ggtgatgtca
      421 tagtgaacaa cgtcagccat aggaggtctc gttccgggtt cggggttcag ggttccaagg
      481 tcccgcgcgg agcatcgacg atgatgcagg tcgtcgtgcc gtgggcgtag agtttgccgg
      541 ccgcgtcgac gatgcggcct tcggacgtcg cggtgcgacc gccgacgtgg accaccttcg
      601 cttcgcaccg cagcagaccg gcttccttcg tgatcggccg ggtcaggttc accttcaact
      661 ccaatgtggt gaagcgacgg cctgccggca tcgccgagtt gatcgcgcag ccgagcgcgg
      721 tgtccagcat cgtcgccgcg aagcctccgt gcgcgacgcc ggtgccgttg tagaagctct
      781 cctcacagac ggccgtgaag acgacccggc cctcttcgac ctcggcgatg cgaatgccga
      841 gcagatcgag catcggcggt ggcggcatct cgccagcgat catccgctgg aagaactcga
      901 gcccgctcat ccgccggcgc gcttcgacgt gggtcatgaa ccggtgcctg cggcgtgttc
      961 atctctcaaa acgcgataaa ggatcttgcc gaccgcgctc atcggcaggg tgtcgcggat
     1021 ttcgacgact cggggcactt tgtaatccgt gaggctgatc cggcagtgtg cgatcacctc
     1081 gtcggccgtc accgacgagc cgggtttgag ggcgatgcag gccttgacga tttcgccgtg
     1141 gtagttgtcc ggaatcccga tgactgccgc gaggcggaca gccgggtgcg cgtacaggac
     1201 cgactcgacg tccgacgggt acacattgta gccgtcgacg atgatcatgt ctttcttgcg
     1261 ctggacaatt gacgtgtagc cgtccgcgtc catgcgcgcg atgtcgccgg tgtggaacca
     1321 gatccggccg tcggcgtgcg tgcgcaggac gtgcgcgctt tcctccggct tgttccagta
     1381 gcctttcatc acctgaggtc ccgcgatgca cagctcgccg gtgtcgccga ccggcaactc
     1441 gtgcgtgccg gtttcgacgt cgacgatctt catgtcggtg tcgggaaacg gcagaccgac
     1501 cgtcccgaac ttccgcaagg cgagctgcgg cgtggagtgc gtgaccggtg aggtttccga
     1561 cagcccgtag ccttcgttga gcggccggcc gatgcgccgt tcgaattcct ccatcacctc
     1621 gaccggacac ggcgcgccgc cgctgttgta caggcgtatc aggtcgagcc cgtactcgcc
     1681 gaccttcgga tggttgagca gcgacacgaa cactgttggc acggcgggga agtaggtcgg
     1741 acggaagtcg cggatcgcgg cgagcacctg ctccggatcg tacttggggt ggatgatctg
     1801 gagcgcgccg acccgcagcc ccaccaacat gcacaccgcg aacgcgtaga tgtggaagta
     1861 cgggatgacc acgaggtacc gctcattgcc gctcagcgta tacgccggat tcatccagtt
     1921 ctcggtctgc acgacgttcg cccacaggtt gccgtgggtc agcatcgccc ccttcggcgt
     1981 cccggtcgtc ccaccggtgt attgcagcac cgcgatgtct tcgggtccga cccgggtcag
     2041 acccgggtct gacccggggc tgccagccgt cgggcccgac gctgacaccg attggcgacg
     2101 agtcaactcc gtcagggaaa gtgtgccgtc gatgcgtggg gtgggcgtgg cggccgctga
     2161 atattctgcc aacgacgtca cgatgacgtg ttcgatggac gtctgactgc ggacaccctg
     2221 caccagcggc gccagcagat ccagtgtgac gacgacgcg
//
LOCUS       DASRJA010000574         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_591672,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000574 DASRJA010000000
VERSION     DASRJA010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591672"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFC01_25365"
     CDS             complement(<1..255)
                     /locus_tag="VFC01_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN82979.1"
                     /translation="MAAQVSAPPAHAVPGPEVEYVYDVMVRRHYDFPNNDAIGYGYGI
                     CDKVAQGEGYPRVMGEVKNDVTPNDEFAANYLVSNAVGILC"
     gene            362..631
                     /locus_tag="VFC01_25370"
     CDS             362..631
                     /locus_tag="VFC01_25370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82980.1"
                     /translation="MSAAGSDSGPGSPGSVAGYWVVKSTSWSKSGAMGSSRQNKTIAK
                     LHGSPTGSASADAGMMSDTVARPIDAKTVDALRLIAPAFLSHEVI"
     gene            complement(624..1511)
                     /locus_tag="VFC01_25375"
     CDS             complement(624..1511)
                     /locus_tag="VFC01_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HZN82981.1"
                     /translation="MMRRVILLRTALVAILGLCLWTAGPAKAAPAIEYLHVPSASMGR
                     DIPVAFMAGGPHAVYLLDAFDAGPEVSNWVTAGEAFTTLAGKGISVAAPAGGAYSLYT
                     NWEQDGSRQWQTFLCDELPNWLAANKGLAPNGHAIVGAAQGGTGAMEAATFRPDRFRY
                     AGSMSGYLAPERTGVDGAITAGLQRYGGVDIRNMWSLPQLGRWKDHSANVHARLLVDN
                     DTRLWVFSPGTLTASDPAAMIGYPDIAQGTNRTFFAQYRAVGGKNGHFDFPTSGDHGW
                     SSWAPQLAAMAGDLATTIR"
     gene            complement(1550..1984)
                     /locus_tag="VFC01_25380"
                     /pseudo
     CDS             complement(1550..1984)
                     /locus_tag="VFC01_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212590.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
     gene            complement(2029..>2301)
                     /locus_tag="VFC01_25385"
     CDS             complement(2029..>2301)
                     /locus_tag="VFC01_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82982.1"
                     /translation="DLLTGTFRDSYTSLTTDVVIPGSQQKKISATATVPAASSMSASE
                     KHAVVLVFVDQTVIVGNDAPSGTASAVQVTLDKVDNRWLISGFDPK"
BASE COUNT          394 a          712 c          761 g          434 t
ORIGIN      
        1 acacaaaata cccaccgcat tcgaaacgag ataattggcc gcgaactcgt cgttgggggt
       61 cacgtcgttc ttgacctcac ccataacccg cggatacccc tcgccttgag cgaccttgtc
      121 gcagatcccg tagccgtaac cgatggcgtc gttgttcgga aagtcgtagt gtcggcgcac
      181 catcacgtcg tagacgtact cgacctcggg acccggaacc gcatgcgccg gcggtgccga
      241 cacctgtgcg gccatcccca atgcgatgca cgcgccgact attctgagca acgacttctg
      301 acacatcacg gctgaaatcc ccttttcctc gtcgcctcgt cagctgcagc ctcgaggaca
      361 aatgtcggcc gccggcagcg actccggccc tggatcgccg ggatcggtcg ccggatactg
      421 ggttgtcaaa tcgacgtcgt ggtccaagtc cggggcgatg ggcagcagcc gacaaaacaa
      481 gacgatcgcc aagctgcacg gatcgccgac cgggtcggcg tccgcggacg ccggcatgat
      541 gagcgacacc gtggcgaggc cgatcgatgc caaaaccgtt gatgcgcttc gtctcatcgc
      601 gccggccttt ctcagccacg aggtcatctg atggtggtgg cgaggtcgcc tgccatggcc
      661 gcgagttggg gtgcccagct cgaccagccg tggtcaccac tggtcggaaa gtcgaagtgg
      721 ccgttcttgc cgcccaccgc gcgatattgc gcgaagaagg tgcggttggt gccctgcgcg
      781 atgtcggggt agccgatcat cgcggctggg tcggacgccg tcaacgtgcc agggctgaag
      841 acccacagcc gggtgtcgtt gtcaacgagg agccgggcat gcacgtttgc cgagtggtcc
      901 ttccagcgcc ccaactgcgg cagactccac atgttgcgga tgtcgacacc cccgtaccgc
      961 tgcagcccgg cggtgatcgc gccgtccaca ccggtccgct cgggcgccag gtaacccgac
     1021 atcgagcccg cgtagcggaa gcggtcgggg cggaacgtcg cggcctccat ggcccccgtc
     1081 ccaccctgcg cggcgccgac gatggcgtgt ccattgggcg ctagaccctt gttggcggcc
     1141 aaccagtttg gcagttcgtc acacaggaac gtctgccatt gcctgctgcc gtcctgctcc
     1201 cagttggtgt agaggctgta ggcaccgccg gcgggggctg ccaccgagat gcccttgcca
     1261 gctagggtcg tgaacgcttc gcccgcggtg acccagttgc tcacttcggg gccggcgtcg
     1321 aaggcgtcga gtagatacac ggcatggggt ccacccgcca tgaacgcaac cggaatgtcg
     1381 cggcccatgg acgccgacgg cacgtgcagg tattcgatgg ccggcgcggc cttggccggt
     1441 ccggcggtcc acaggcatag tccaaggatg gccaccagcg ccgtcctcaa caggattact
     1501 ctgcgcatca taggtattgg ctccgaagaa accggtcgtc gagtcatgcg ggctggccat
     1561 agacggcgac gacgacggtg cgatcgaggc tgttctgcga ccacactccg atctgggtgt
     1621 tggcgcagtt gctcatgatc gccatgtcgg tggggtcgta gtaccactgg ttgaggatct
     1681 cgataccgct gatcgccaac gcggggttga tcgccacggt ttcggcgacg gttccgcggt
     1741 aaccggcggc gttggcgcgg tccgcaacgg tggtgccgtc ggatccgatg tcaccatcga
     1801 gcgctctgtt gttgaggacg tcgtcggtat gccattgggc agccaactgc agttgcgggt
     1861 tgatcttgag gtcgttcgtg cagccggctt ggtgctgaat ggtgtacacg ttggccgcga
     1921 cactgttgtt gagccggtga ttgtcggcac gggcctgcgg cgtgatcgcc agtgcgccca
     1981 ccattacgag cgtcggaacg gtaagcgccg caatcgcgtt ggcggatctc atttgggatc
     2041 gaaacccgat atgagccaac ggttgtcgac tttgtcgagg gtgacttgga ctgcggacgc
     2101 ggtgccgctg ggcgcatcgt taccgacaat cacggtttga tcaacgaaga ccaggacgac
     2161 cgcatgtttc tcgctggccg acatcgacga ggcggcgggc acggtggcgg tggcggagat
     2221 cttcttctgc tgtgatccgg ggatcaccac gtccgtggtg agggacgtgt aggagtcacg
     2281 gaaggtgccc gtcagaaggt c
//
LOCUS       DASRJA010000575         6176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3978766,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000575 DASRJA010000000
VERSION     DASRJA010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3978766"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFC01_25390"
     CDS             complement(<1..655)
                     /locus_tag="VFC01_25390"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZN82983.1"
                     /translation="MRQDRVAPRRHGARSKGIPALDGIRAVAVGLVLAEHGGIPGVSG
                     GFLGVDVFFVLSGFLITSLLLDELRRTGRIALADFWIRRARRLLPALIVMVLAVVAAR
                     TVFPTEAITTLRDDAVAVFFWVANWAFVAQRTDYFSQGSPPSPLQHTWSLGVEEQYYL
                     FWPLLVIAVAALFGARARWAVFALATAGVAGSAAGAIVLASDVNRVYFGTDTRVQALL
                     "
     gene            complement(708..2156)
                     /locus_tag="VFC01_25395"
     CDS             complement(708..2156)
                     /locus_tag="VFC01_25395"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZN82984.1"
                     /translation="MDTTALLKSVPTGLWIGGEEREGKSTFNVLDPSDDQVLVAVADA
                     TPEDATAALDAACAVQAEWAATPPRERGEVLRAVFEKITERAEDVATLMTLEMGKVLP
                     ESMGEVKYGAEFFRWFAEEAVRIAGRFTPSPAGTGRILVTKQPVGPCYAITPWNFPLA
                     MGTRKIGPAFAAGCTMIVKPAQETPLTMLLLAKLMDEAGLPKGVLSVLPTSSPGPITE
                     ALINDGRLRKLTFTGSTGVGKALVKQSADKLLRTSMELGGNAPFVVFDDADVDAAVDG
                     AILAKMRNGGEACTAANRFHVANSVREEFTEKLVKRMSEFTLGKGIDESSTLGPLINA
                     KQVATVEDLVSDAVSRGAAVAVGGVAPGGPGNFYPATVLADVPADARILKEEVFGPVA
                     PITGFDTEEEGIKAANDTEYGLAAYIYTQSLDRALRAAEGVQSGMVGINRGVISDPAA
                     PFGGVKESGFGREGGFEGIDEYLDVKYIALTK"
     gene            2284..3951
                     /gene="pgi"
                     /locus_tag="VFC01_25400"
     CDS             2284..3951
                     /gene="pgi"
                     /locus_tag="VFC01_25400"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZN82985.1"
                     /translation="MSTDTQQIPDIATTPAWQALQKHHDEIGQKHLREFFADDPNRGT
                     ELALTVGDLYIDYSKHRVTRETLQLLIDLARTARLEQHRDAMFSGIHINTSENRAVLH
                     TALRDPAGVELVVDGQNVVEDVHAVLDKMGDFTDRLRSGEWTGATGERIKTVVNIGIG
                     GSDLGPVMVYDALRHYADAGISARFVSNVDPADLVATLDGLEPATTLFIVASKTFSTL
                     ETLTNATAARRWLTETLGDAAVAKHFVAVSTNKKLVDDFGINTDNMFGFWDWVGGRYS
                     VDSAIGLSVMAVIGRERFADFLAGFHIVDEHFRTAPLEANAPALLGLIGLWYNNFFGA
                     ETRAVLPYSNDLSRFAAYLQQLTMESNGKSVRADGTAVTASTGEIFWGEPGTNGQHAF
                     YQLIHQGTRLIPADFIGFSQPTDDLATADGTGSMHDLLMSNFFAQTQVLAFGKTAEEI
                     AAEGTPPEVVPHKVMPGNRPSTSILGTKLTPSIVGQLIALYEHQVFTEGVIWGIDSFD
                     QWGVELGKTQAKALLPVITADASPAKQSDSSTDALVRRYRTERGRSA"
     gene            4095..5144
                     /locus_tag="VFC01_25405"
     CDS             4095..5144
                     /locus_tag="VFC01_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016881468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZN82986.1"
                     /translation="MHARSVVACGLDGDTGELFERRLTPDHGEIVAWLGDLPGPVAAT
                     YEAGPTGFVLARSINAAGIRCSVAAPSKLQRPVGDRVKTDIRDARHLARLLHLGEIVE
                     VEIPSVEQESARDLFRAREACRRDLMAARNRLSKLLLRRGIVYYGGAAWSRNHERWLR
                     GQRFDDPALAIAYDTAFDTVLATTARRDRLDAAITAMAADSSFTAVMTRLGCLRGVST
                     LTAFGLATEIGDWHRLTGRSIGAYLGLVPCEYSSGANRAQGGLTRTGNTHARRLLIEA
                     AWHHRPSYRPGEVMRRRWDAASPAARARGQAANRRLHARWLRFNERKKRPVVANAAIA
                     RELAGWCWSLAVMDD"
     gene            complement(5462..6043)
                     /locus_tag="VFC01_25410"
     CDS             complement(5462..6043)
                     /locus_tag="VFC01_25410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN82987.1"
                     /translation="MVADHPLTPDKLHWLARKLNHFGGEAISEEGVEFDGDDVNWSDV
                     TEIHTRSLIEYLFTGGVDTQIDKLPLPWFPFRRKVIEAISRAALTLLLAAAKQQLEGG
                     ALDIRIPAEVCHRGLLDTRELSPGMIAAVVLADPAVRQCFEATAQAHGVEIRPAEDDV
                     MADADQRAAQIKSMLDVISVRIRTLSDSTLSDG"
BASE COUNT         1068 a         2062 c         2035 g         1011 t
ORIGIN      
        1 tcagcaatgc ctgcacccgg gtgtcggtgc cgaaatagac gcggttcacg tcagacgcca
       61 atacgatcgc cccggcggcc gagcccgcaa cgcccgcggt cgcgagtgcg aagacggccc
      121 accgggcgcg ggcgccgaac aacgccgcga cggcgatcac cagcagtggc cagaacaggt
      181 aatactgctc ctcgacgccg agcgaccacg tgtgctgcag cggcgaaggt gggctgccct
      241 gcgaaaagta atcggtccgc tgtgcgacga aggcccagtt cgccacccag aagaagacgg
      301 cgacggcgtc gtcgcgcagc gtggtgatgg cctccgtcgg aaaaactgtg cgcgccgcga
      361 ccaccgctaa caccatgacg atcagcgcgg gcagcagccg tcgggcacgt cgaatccaga
      421 agtccgccaa cgcgattcgc ccggtgcgcc gcagttcgtc gagcaacagc gacgtgatca
      481 gaaatccgct gagcacgaag aagacgtcga cacccaagaa gccgccggag acaccgggga
      541 tgccgccgtg ctcggccaac acgagtccga cggcgacagc gcggatgccg tcaagcgccg
      601 gaatgccttt agaccgagcg ccatgccgac gcggggcgac acggtcctgg cgcactgcgc
      661 gaagtttagg tggcctcccg cgccgaatcc ggaaggcgcg ctgggcttta cttcgtcaaa
      721 gcgatgtatt tgacgtcgag gtactcgtcg atgccctcga agccgccctc acggccgaag
      781 ccggattcct tgaccccgcc gaatggagca gccggatcgg agatgacgcc gcggttgatg
      841 cccaccatgc ccgactggac tccttcggcc gcccgcagcg cgcggtccag cgactgggtg
      901 tagatgtagg ccgccaaccc gtattcggtg tcgttggccg ccttgatgcc ttcttcctcg
      961 gtgtcgaagc cggtgatcgg cgcgacgggc ccgaacacct cctccttgag gatgcgggcg
     1021 tcggcgggga cgtcggccag cacggtcgcc gggtagaagt tgccggggcc gccgggagcg
     1081 acaccgccga cggcgacggc cgcgccgcgc gacaccgcat cggacaccag gtcctcgacg
     1141 gtggcgacct gcttggcgtt gatcagcgga cccagcgtgg acgactcgtc gatgcccttg
     1201 ccgagggtga actcgctcat ccgcttgacc agcttctcgg tgaactcctc gcgcacggag
     1261 ttggcgacgt ggaaccggtt ggccgcggtg caggcctcac cgccgttgcg catcttggcc
     1321 aggatcgcgc cgtcgacggc ggcgtccacg tcggcgtcgt caaacacgac gaacggcgca
     1381 ttgccgccga gttccatcga tgtgcgcagc agcttgtcgg ccgactgctt gacgagtgcc
     1441 ttgccgacgc cggtggatcc ggtgaacgtc agcttgcgca gccggccgtc gttgatcagc
     1501 gcctcggtga tcggcccggg actgctggtc ggcagcaccg acagcacccc cttcggcagg
     1561 cccgcctcgt ccatcagctt ggccagcagc agcatggtca gcggcgtctc ttgagccggc
     1621 ttcacaatca tcgtgcagcc cgccgcgaac gccgggccga tcttgcgggt ccccatggcc
     1681 aacgggaagt tccacggcgt gatcgcgtaa cacgggccga cgggctgctt ggtgaccagg
     1741 atgcggccgg ttccggccgg gctcggagtg aaccggccgg cgatgcgcac cgcctcctcg
     1801 gcgaaccagc ggaagaactc cgcgccgtat ttgacttcgc ccatgctctc gggcagcacc
     1861 ttgcccattt ccagggtcat caacgtcgcg acatcctcgg cgcgttcggt gatcttctcg
     1921 aagaccgcac gcagcacctc gccgcgttcg cggggcggcg tggcggccca ctccgcctgc
     1981 acggcgcagg cggcatcgag cgcggcggtc gcgtcctcgg gggtggcgtc ggcgacagcg
     2041 acgagcacct gatcgtcgga cgggtcgaga acgttgaatg tcgacttgcc ttcgcgttcc
     2101 tcaccaccga tccacagacc ggtgggtact gacttcagca gagcggtggt gtccatgcct
     2161 ccatcattta cacctttgtt caaccggccg cactagggtc gaaaacatga gcacccgaag
     2221 acgagcgcga gtgaggatcg cagcgaggga cgagcgagga ccggagcgag cgggagtcga
     2281 ggcatgagca ccgataccca gcagattccc gatatcgcca ccactcccgc gtggcaggca
     2341 ctgcaaaaac atcacgacga gatcggtcag aaacacctgc gggagttctt tgctgacgac
     2401 cccaaccgcg gcaccgaatt ggcgttgacg gtcggcgacc tctacatcga ctacagcaag
     2461 caccgggtca cccgggaaac cctgcaactg ctgatcgacc tggcccgcac cgcccggttg
     2521 gagcagcacc gcgatgcgat gttctccggc atacacatca acacctccga gaaccgggcg
     2581 gtgctgcaca ccgcgctgcg cgatccggcc ggcgtcgagc tggtcgtcga cgggcagaac
     2641 gtcgtcgagg atgtgcacgc ggtgctggac aaaatgggcg acttcaccga ccggctgcgc
     2701 agtggcgagt ggacgggcgc gaccggcgag cggatcaaga ctgtcgtgaa catcggcatc
     2761 ggcggctcgg atctgggccc tgtgatggtg tacgacgcgt tgcgccacta cgccgacgcg
     2821 ggcatctcgg cgcggttcgt ctcgaacgtc gaccccgccg acctggtggc cacgctcgac
     2881 ggactcgagc ctgccacaac gcttttcatc gtcgcatcga agacgttctc cacactggag
     2941 acgctcacca acgcgaccgc cgcgcggcgc tggctgaccg agacgctcgg agacgccgcc
     3001 gtggcaaagc atttcgtcgc ggtctcgacc aacaagaaac tggtcgacga cttcggcatc
     3061 aacaccgaca acatgttcgg cttctgggac tgggtcggcg gccgctattc ggtggacagc
     3121 gcgatcggtt tgagcgtgat ggcggtgatc ggcagggagc ggttcgccga cttcctggcc
     3181 ggcttccaca tcgtcgacga gcacttccgc accgcgccgc tggaggccaa cgcgccggcg
     3241 ctgctcggtc tgattggctt gtggtacaac aacttcttcg gcgccgaaac gcgtgcggtg
     3301 ctgccgtatt ccaacgacct gtcgcggttc gccgcctatc tgcagcagct gacgatggag
     3361 tccaacggca agtcggtgcg cgcggacggc acagccgtga cggccagcac cggcgaaatc
     3421 ttctggggcg aaccgggtac caacggccag cacgcgttct accagttgat acaccaggga
     3481 acgcggctga ttcctgctga cttcattggg ttttcccagc cgaccgacga cctggccacc
     3541 gccgacggca caggcagcat gcacgacctg ttgatgagta acttcttcgc ccagacgcag
     3601 gtgctcgcgt tcggcaagac cgcagaggag atcgccgcgg agggcacgcc gccggaagtc
     3661 gtgccgcaca aggtgatgcc gggcaaccgg ccgtcgacgt cgattctggg caccaagctg
     3721 acgccgtcga tcgtgggcca attgattgcc ctctacgagc accaggtctt caccgagggc
     3781 gtcatctggg ggatcgactc gttcgaccaa tggggcgtcg aactgggcaa gactcaggcc
     3841 aaggcgctgc tgccggtgat cacggctgac gcgtcaccgg ccaagcagtc cgattcgtcg
     3901 accgacgcct tggtgcggcg ctaccgcacc gaacgcggac ggtccgccta gacggtatgg
     3961 ggggctgggg cctcgctcac atgtgaacgt gggttgccgt caggaaatgg gtcctggccg
     4021 gtagagccac agatgtggga ggccccagtg aaggttcagc gtacgaggtt cagcgtacga
     4081 gtgttggttt ggatgtgcac gcacgatcgg tggtcgcgtg tggtcttgat ggtgataccg
     4141 gggagctctt cgagcggcgg ttgactccgg atcacgggga gatcgtggcc tggttgggtg
     4201 atctgcccgg cccggttgct gcgacctatg aggccggtcc gacgggattc gtgctcgcgc
     4261 gcagcatcaa cgccgccggg atcagatgct cggtcgcggc gccgtcgaag ctgcaacgcc
     4321 cggtcggtga tcgggtcaag accgatatcc gcgatgcgcg tcatctggcc cggctgctgc
     4381 atctgggtga gatcgtggaa gtcgagatcc ccagtgtcga gcaagaatcc gcacgcgatc
     4441 tcttccgcgc ccgtgaggcc tgccgccggg atctgatggc cgcacgcaac cggctctcga
     4501 agctgctgct gcgcaggggg atcgtctact acggcggggc ggcctggtcg cgaaatcacg
     4561 agcggtggct gcgcgggcag cggttcgacg accccgcgct ggcgatcgcc tacgacaccg
     4621 ccttcgacac ggtgctggcc accaccgctc gccgggatcg cctcgacgcg gcgatcaccg
     4681 cgatggccgc cgattcctcg ttcaccgcgg tgatgacgcg gctggggtgt ctgcgcggcg
     4741 tctccacgtt gacggcgttc gggttggcca ccgagatcgg cgactggcac cggctgaccg
     4801 gccgctcgat cggcgcctat ctcggactgg tgccatgcga gtactcctcg ggagccaatc
     4861 gggcccaggg cgggctgacc cgcaccggca acacccacgc ccgccgactg ttgatcgagg
     4921 cggcctggca tcaccggccg tcgtatcggc ccggcgaggt gatgcggcgg cgctgggatg
     4981 ccgcctcacc ggcggcgcgg gcccgcgggc aggccgccaa ccgacgcctg cacgcccgct
     5041 ggctgcgttt caacgaacgc aagaagcgtc ccgtcgtggc caacgcggcc atcgcccgcg
     5101 agttggccgg ctggtgctgg tcattggccg tcatggacga ctgagcccac cgaaacgtcc
     5161 aggtgcgcgt atcgaggtgt cggcaagcgt cttggagtga cccgcggaaa tcgctatgag
     5221 caatcacgtt gccctgcaac cgatcacgct cgattactag accctgcgat ccaactccga
     5281 ctcgaagacc cgtcctgcgg taaccaaccc gcgcatgtca gactgacacg cgtcgacaga
     5341 cacgctcgac acccacgaac gctcacctgg tcaacgaagc ggcggccgcg acgacggttg
     5401 cggccgccgc ttcaccctgc ccacttgaca aaccactccc catatcagcg atttcggtgc
     5461 gctaaccgtc gctcagcgta ctgtcgctca gcgtacgtat tcgcaccgaa atcacgtcga
     5521 gcatcgactt gatctgtgcg gcgcgttggt cggcatccgc catcacgtcg tcctcggcgg
     5581 gacggatctc gacgccgtgc gcctgcgcgg tcgcctcgaa acactgccgg accgcagggt
     5641 cggcgagcac cactgcggcg atcatgcccg gtgacagttc gcgggtgtcg agcaagccgc
     5701 ggtggcacac ctccgcgggt attcggatgt cgagcgcacc accttcgagc tgctgtttgg
     5761 ccgccgcgag tagcagcgtc agcgccgccc gcgagattgc ctcgatcacc ttgcggcgga
     5821 acgggaacca cggcagcggt agcttgtcga tctgcgtgtc cacgccgccg gtgaacagat
     5881 actcgatcag gctgcgggtg tggatctcgg tgacatccga ccagttgacg tcgtcgccgt
     5941 cgaactcgac gccctcctcg ctgatcgcct cgccgccgaa gtggtttagc ttgcgggcca
     6001 gccaatgcag tttgtctggt gtcagcggat gatcggccac catgtcgccg atgcccagcg
     6061 cctaccgacc gacgacgggc ccggatcggg tcgccgggtt tccagcagcg actgcaacca
     6121 cgaggcctcg tcgggcacct cgccatttaa gccgaattcg ctactgcagg cagttc
//
LOCUS       DASRJA010000576         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1785340,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000576 DASRJA010000000
VERSION     DASRJA010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785340"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1702
                     /locus_tag="VFC01_25415"
     CDS             185..1702
                     /locus_tag="VFC01_25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN82988.1"
                     /translation="MTATPDVDLPDTSTIRNPATGAVAGRVRWTDPADVPRIAAGLRE
                     VQKHWEARGAKGRAKVMARYAVWLGEHRDEIEALLIKETGKSATDAAQEVPLLIMIAS
                     YYIKTMEKALAPEPRPASLPFLAIKKVEVHYRPRPVVGIIAPWNYPVANALMDAIGAL
                     AAGCAVLLKPSERTPLTAELLLRGWLDSGAPEVLALAQGAREVSEVVIDNSDFIQFTG
                     SCATGAKVMERAARRLTPVSLELGGKDPMVVLEDADIDLAAHAAVWGAFFNAGQTCVS
                     VERVYALEPVYDQFVAAVMRDVKDLKVGAGDGYDFGSLIDESQVAVTERHVADAKAKG
                     ARVLTGGRRPSGPGSFYPPTVLVDVDHSMACMTEETFGPTLPIMKVSSVDEAIRLAND
                     SPYGLSAAVFSKDIERAKAVAVQLDCGAVNVNDVISNLMCTTAPMGGWKTSGMGARFG
                     GAEGLRKYCRQEAVVAPRTNVGAGGNYYNNSPKALRRMNKLMTKLALLTPRRAAK"
     gene            1761..>2411
                     /gene="ppk2"
                     /locus_tag="VFC01_25420"
     CDS             1761..>2411
                     /gene="ppk2"
                     /locus_tag="VFC01_25420"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HZN82989.1"
                     /translation="MTLDSHGYRVHDDDDDDPVLLNSDGIPVDTWREGYPYDERMNRT
                     EYEEQKRLLQIELLKLQKWSQSNGHRHVIVFEGRDAAGKGGTIKRFMEHLNPRGARVV
                     ALDKPTEEERGQWYFQRYVGHLPTRGEIVLFDRSWYNRAGVERVMGFCTDKEYLEFMR
                     QAPVFEQNLVHSGIHLIKFWFSVSRGEQRRRFGEREAHPLKQWKLSPIDKASLDKWD"
BASE COUNT          434 a          761 c          829 g          387 t
ORIGIN      
        1 gcctgctcgg catgatcgga cagggcatca cgtaccacta cttcaccgat acggtcggtg
       61 cgctgctgtt gggcacggcg atcgtatgcg tcgcagccct cgtattggga cacacacccc
      121 accaaacttg acaggtgtca accccggtgc gatctgcgtc acagccggtg gttacgatga
      181 ggccatgacc gcgacgcccg acgttgatct gcccgacacc agcaccatcc gcaatcctgc
      241 gaccggcgcg gttgccggtc gggtgcgctg gaccgacccc gccgacgtgc cgcgcatcgc
      301 cgcgggtctg cgcgaggtgc agaagcactg ggaggcacgg ggcgccaagg gtcgcgccaa
      361 ggtgatggcc cgctacgcgg tctggctcgg tgagcaccgc gacgaaatcg aggcattgct
      421 gatcaaggag accggcaagt cggctaccga cgccgcgcaa gaggtaccgc tgctgatcat
      481 gatcgcctcc tactacatca agacgatgga gaaggcgctg gcgcccgaac cccgtccggc
      541 atcgctgccc ttcctggcga tcaagaaggt cgaggtgcac taccggccgc gtccggtggt
      601 cggcatcatc gcgccgtgga actaccccgt cgccaatgcg ctgatggacg ccatcggtgc
      661 gttggctgcc ggctgcgcgg tgctgctcaa gccgtccgag cgcacgccgt tgacagccga
      721 actgctgctg cgcgggtggc tggactcggg tgcacccgag gtgctcgcgc tggcgcaggg
      781 cgcgcgcgag gtgtcggagg tcgtgatcga caactccgac ttcatccaat tcaccggctc
      841 gtgcgccacc ggcgcgaagg tgatggagcg cgcggcacgc aggcttacgc cggtcagcct
      901 cgagctcggc ggcaaggacc cgatggtcgt gctcgaggac gccgacatcg acctggccgc
      961 gcacgccgcg gtgtgggggg cgttcttcaa cgcgggccag acatgcgttt cggtggaaag
     1021 ggtgtatgcg ctcgagccgg tgtacgacca gttcgtcgcg gcggtgatgc gcgacgtcaa
     1081 agacctgaag gtgggcgcgg gcgacgggta tgacttcggc tcgctgatcg acgagagcca
     1141 ggtggcggtg accgaacgcc acgtcgccga cgcgaaggcc aaaggtgccc gggtgctcac
     1201 cggtgggcgc cgtccgagcg gccccggcag cttctacccg ccgacggtcc tcgtcgacgt
     1261 cgatcattcg atggcgtgca tgacggagga gacgttcggt ccgacgctgc cgattatgaa
     1321 ggtatcgagt gtcgacgagg cgatccgact ggccaacgac agcccgtacg ggttatccgc
     1381 cgcggtgttc tccaaggaca tcgagcgcgc gaaagcggtt gcggtgcaat tggattgcgg
     1441 tgccgtcaac gtcaacgacg tgatttcgaa cctgatgtgc acgacggcgc cgatgggtgg
     1501 ctggaagaca tccggcatgg gcgcgcggtt cggcggggcc gagggcctgc gcaagtactg
     1561 ccgccaggag gcggtggtgg cgccgcgcac caatgtcggc gccggcggca actactacaa
     1621 caattcgccc aaggcgctgc ggcggatgaa caagctgatg acgaagctgg cgctgcttac
     1681 gcctcgacgc gcagccaagt agcaccggcc gttcacccgc gtgtcaccta ggcgtacacg
     1741 cgcgtcgata ttttttgccg gtgactctgg attcacacgg ctaccgcgtg cacgacgacg
     1801 acgatgacga cccggtgctg ctcaactccg acggaattcc cgtcgacacc tggcgtgagg
     1861 gctatccgta cgacgagcgg atgaaccgca ccgaatacga ggagcagaag cggctgctgc
     1921 agatcgagct gctcaagctg cagaagtgga gccagtccaa cgggcaccgg cacgtcatcg
     1981 tgttcgaggg tcgcgacgcc gcaggcaagg gcggcaccat caagcgcttc atggagcacc
     2041 tcaatccgcg cggtgcgcgc gtggtcgcgc tcgacaagcc caccgaggaa gagcgcgggc
     2101 agtggtattt ccagcgctac gtgggtcacc tgcccactcg cggcgagatc gtcctattcg
     2161 accgctcctg gtacaaccgc gcaggcgtgg agcgggtgat gggattctgc acggacaagg
     2221 aatacctcga gttcatgcgc caggcgccgg tgttcgagca gaacctcgtg cacagcggca
     2281 tccatctcat caagttctgg ttctcggtga gccggggcga gcagcggcgc cgcttcggcg
     2341 agcgcgaggc gcatccgctg aagcaatgga agctgtcgcc gatcgacaag gcgtccctcg
     2401 acaagtggga c
//
LOCUS       DASRJA010000577        11050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1482407,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000577 DASRJA010000000
VERSION     DASRJA010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11050
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482407"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..857
                     /locus_tag="VFC01_25425"
     CDS             9..857
                     /locus_tag="VFC01_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN82990.1"
                     /translation="MQRKSILVDGLVTSYLEAGQGDPVVLLHGGEFGVSAEIGWERNI
                     EALAAHHRVFALDMLGFGGSAKVIDFNDGRGMRIRHIARFCDTVGVETAHFVGNSMGA
                     INLFVDVTSESPVLPARSLVMICGGGEIQRNEHSAALYDYDATLSGMRRILEALFHDP
                     SYPADDAYVQRRYESSIAPGAWEALAAARFRRPGLEPPALPSSARPYERIAVPTLVVE
                     GECDKLLPPGWAAEIAAQIPDGRSIVVPKAGHCPQIEQPSVVNHVLVDFLSGVQRKVP
                     ASWSTN"
     gene            842..1642
                     /locus_tag="VFC01_25430"
     CDS             842..1642
                     /locus_tag="VFC01_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN82991.1"
                     /translation="MVNELTDKVAIVTGGASGLGEGLVRRFAAEGAKVLIGDIDVERG
                     KALAAELGQNALFVEADVADFGQVGGLVSSAVEHFGGLHVMVNNAGVSGTMHRRFFDD
                     DLADFHQVMAINVGAVMAGTRDAARHMSKHGGGSIINLTSIGGIQAGGGVMTYRASKA
                     AVIQFTKSAAIELAHYEIRVNAIAPGNIRTAIVRKSAAGEDLEKLEQFEAKIRQQMRD
                     DRPLKREGTVEDVAEAALYFATDRSRYVTGTVLPIDGGTVAGKVIPRK"
     gene            1639..2349
                     /locus_tag="VFC01_25435"
     CDS             1639..2349
                     /locus_tag="VFC01_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN82992.1"
                     /translation="MTCLNQSLDLRRGTALTRQVTTARTVRADRASSTQEAILKAAER
                     LYAEHGMFAVSNRQVSEAAGQGNNAAVGYHFGTKTDLVRAIEQKHRASIERLLERMVA
                     ETGDSAELRDWIACLVCSLTEHLAQLGNPTWYARFAAQALTDPAYHKIVIKDALASPS
                     LVQVVDGITRCLPNLPAAVVTERNIMARNLMMHTCADFERAYAEGADVPRTSWEAVGS
                     GLIDAIVGLWLAPVTEHP"
     gene            2351..2545
                     /locus_tag="VFC01_25440"
     CDS             2351..2545
                     /locus_tag="VFC01_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN82993.1"
                     /translation="MKVTVDQDKCVSSGQCVLNAAEIFDQRDDDGVVVLLNENPPPPQ
                     AENARKAAAACPALAIDIEE"
     gene            2556..3785
                     /locus_tag="VFC01_25445"
     CDS             2556..3785
                     /locus_tag="VFC01_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN82994.1"
                     /translation="MSDTLTGTATEVPDYPMDRDARCPFAPPPQVLEMGEAKPLSRVR
                     IWDGSTPWLITGHAVARELFADSRVSVDDRQDGFPHWNEHMLSTVHKRPRSVFTSDAE
                     EHTRFRRMLSKPFTFKRVEGLRPAIQEVTDECIDAILAGPQPADIVSKLALPVPTRVI
                     SEMLGVPYEDHEFFQHHANVGLARYATAEDGQKGAMGLAQYLIDLVKKKMENPSEDAV
                     SDLAERVNAGEISVKEAAQLGTGLLIAGHETTANMIGIGVLALLENPEQAVLLRDSDD
                     PKFIANAVEELMRYLSIIQNGQRRVAIEDIEIAGETIRAGEGIVLDLAPANWDATAYP
                     DPDKLDLTRDAGQQLGFGYGRHQCVGQQLARAELQIVFHTLLRRIPTLRLAVPLDEVP
                     FKHDRLAYGVYELPVTW"
     gene            3800..4969
                     /locus_tag="VFC01_25450"
     CDS             3800..4969
                     /locus_tag="VFC01_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN82995.1"
                     /translation="MSTSASTLYPPGGYGAPKDRRGHAGAPVGLPAGTEVFSADNHIS
                     VADDIFYERFPDDLKDKAPRIWYEDGAYMVGMAKGKTFLPGDFSRVLMQYDDLAGAST
                     NNIEARVRELAEDGVDKELAFPNAVLALFHYPDKDLRERVFRIYNEYMADLQERSNGH
                     FYGAGLINWWDPKGARRTLTELKALGLKTFLMPINPGKDDGDKVIDYGSTVMSAVWDE
                     IEEAGLPVSHHIGETPPKTPCEFNSVVVGMMINIDGFREVFSKYIFSGILDRHPKLRI
                     GWFEGGIAWVPWALQDAEHLLASYQHMFNHNLEHDIQYYWDTHMSASFMVDPLGLQLI
                     DRIGVDKVFWSSDYPHNESTFGYSEKSLASVVEAVGPDNAAEIVSGNIKNFLGIE"
     gene            4966..6201
                     /locus_tag="VFC01_25455"
     CDS             4966..6201
                     /locus_tag="VFC01_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZN82996.1"
                     /translation="MTAFATAVTSAHDIPDLPDRARMYRESGARLRSAMADNGIDALI
                     LLGNGNVVYATGASWPLLDAGLSHVERPVAIVLADDEHPHLFMPFREGAPWESQIPAD
                     HLHPPLFLEFDEGVDHFARVLGDLVPASATIAVDELTGAMRRAGDRLFPGGPPSDAAP
                     VVGTAKLVKTVDQISCIRRACRITDEAAVDVQKALAPGVRQIDLSAQFVRRAFELGAT
                     ASMLEPIWQVMPATKAEGTWTTHGDLALPLLTTERELAKGDVLWTDVSITYTGFCSDF
                     GRTWLVGETPSARQQDQFRQWRHILDAVLKVTKAGATSGDLARAAIAANGGQKPWLPH
                     FYLGHGIGTNAAEMPMIGTDLGEEFDDNFVFPAGMVLVLEPVVWEDGTGGYRSEEIVV
                     ITDDGHVSLTNYPYAPYGD"
     gene            6191..7327
                     /locus_tag="VFC01_25460"
     CDS             6191..7327
                     /locus_tag="VFC01_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZN82997.1"
                     /translation="MATEVLPDDRALRIGRRERALAQMEAHDLDVLVLGRQANVRYVT
                     GAPQLWVAGTRPFGPICMVVRSTGEIHLNSTWDEGMPDDIPHDRLYGLAWNPMTLIEV
                     LKSIDGASTARRVGTDALTPTFAKLLPMAFPNAELVDAEPAMRAARRIKTPDEVAALR
                     GALRVAEKSLATAVAELRVGVTAQELTGILLEAEAAGGVSTPATQDGAWVTSREHPWR
                     RARSDGRIENGDLVAFSAGVLADGYVGEVGRTWPVGDVDASALYDRWNTLWDRLAAAC
                     QPGRPAGDLLAAYEAANEPLPPTPVAHGLGLGFDPPVVTPDLRITAAEEILEPGMVLS
                     VTGYVWQEGVGAVFGREAVLVTSDRPEVLTSSPSWSIRAGVGIG"
     gene            7338..8300
                     /locus_tag="VFC01_25465"
     CDS             7338..8300
                     /locus_tag="VFC01_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN82998.1"
                     /translation="MSDSRPKPEELILYEKDPKTKIATITFNRPEYLNAPTSAARLRY
                     ADLLRAATVDNDVKVVVIRGVGDNLGSGADLPEFMEGNDNPELRLAELRLEDEGVGEV
                     SYPPKGSFRHGATISAWYANVQAGNRPLQELKKISIVEAKGYCYGWHFYQCADADLVI
                     SSDDALFGHPSFRYYGWGPRMWTWVQMMGLRKFQEMVFTGRPFTAEEMYQCNFLNKVV
                     PREALEAEVDKYARACARNRPVDTVFQQKLFFEVFKQHQGEYMGSLLSAFFESMGSGV
                     VNDDSDDLDMYEAIDSGLSDAVNDNDSKFPPDFRLSKSNRKKKD"
     gene            8300..10675
                     /locus_tag="VFC01_25470"
     CDS             8300..10675
                     /locus_tag="VFC01_25470"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN82999.1"
                     /translation="MHPLDGYVVVDLSTGIAGAYCTKLLADGGAEIVKIEPPEGDSLR
                     RWSASGAEIRPGDDGALFSYLACSKRSVAADPDVDGDFVNALLADADAVVWSRGSTIA
                     DAFAPAEIHRNHPHLTVTSITPFGIEGPWADRPATEFTLQAWSGGIVGLGRGSADRAP
                     VFVGGQVGDYLAGAYASVATMASRMRGAAELIDLSMLEAQILGLTYYPVTYYEMLGRP
                     WRDVRRLTVPGIARAKDGLIDIGCGTAQQWFDLCAMTGHQDWIDEESPLSITEQANEN
                     ADELYAWVESQTVDEIRDLATAFRIPNVPVANGANIESLDHFQARGSFIANPRDGFTQ
                     PGPPYRMQPAQLRAPQPAPRLGEHTDQHRQRGRRNRDTLPTMPSFEGLRVVDMTTFWA
                     GPSCTHMLAMLGAEVIHVESTRRPDGTRLIAGIPITEDQWWEKSPIFSGLNTNKKGLT
                     LNLQSDAGREVLMQLIATCDVIVENFTPRVVDQIGLDFAAVQAVRPDAIMLRMPGFGL
                     DGPWRDNSAFAYVIEAASGVSWLTGYPDRNPYEPYSVGDPNAGVHALNALLLALEHRR
                     RTGEGVLIEAAMVDAALNIAAEQVIEYSAYGALLRRDGNRGPTAAPQNLYRTNEIDEF
                     GRRDCWVAVAVATDEQWAGLCTAVDKPAWATEIPTAEARRAQEDIIDEHLAAWCGQRT
                     GDDIVEALWSHDVPVAKVMQPHRQSELPQLAARGFFEDVDHPVNATTLHSTLPFTSTR
                     GADRIHTQPAPLLGQHNREILIELGLSDKDIAELETEGVIGTAPAMYGTKV"
     gene            10689..>11050
                     /locus_tag="VFC01_25475"
     CDS             10689..>11050
                     /locus_tag="VFC01_25475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN83000.1"
                     /translation="MAIDPSHILLTGRVAVVTGGGGGIGRGIAEGLAAFGASVTIWEQ
                     NAETCTSAAEAIGALGIVTDVRNSEQVDAALQRTTTELGIPSILVNNAGGVFKAPLLD
                     TTENGWDALYKSNLRHVLL"
BASE COUNT         2053 a         3545 c         3627 g         1825 t
ORIGIN      
        1 tgaccgaaat ccaacgcaaa tcgattctgg ttgacggact ggtcaccagc tatctggaag
       61 cgggacaagg cgatccggtc gtcctgctgc acggcggaga gttcggcgtc agcgccgaga
      121 tcggctggga gcgcaacatc gaggcgttgg cggcacacca ccgcgtgttc gccctcgaca
      181 tgctggggtt cggcggatcc gcgaaggtca tcgacttcaa cgacggccgg ggcatgcgga
      241 tcaggcacat cgcgcgattc tgtgacaccg tcggcgtcga gaccgcacac ttcgtcggta
      301 actcgatggg cgccatcaac ttgttcgtcg acgtcacatc ggagtctccg gtgttgccgg
      361 cgcgcagctt ggtgatgatt tgcggtggcg gcgagatcca gcgaaacgaa cactcggcag
      421 cgctctacga ttacgacgca acgctttccg gaatgcgccg cattctcgag gcgctcttcc
      481 acgacccgtc ataccccgcc gacgacgcct acgtgcaacg gcgctacgag tccagcattg
      541 cgcccggtgc gtgggaggcg ctggcggccg cgcgctttcg gcggccgggc ttggagccac
      601 ccgcactacc gtcgagcgcc cggccctacg agcgcatcgc cgtacccacg ctggtggtgg
      661 agggggagtg cgacaaattg cttcctccgg gctgggcggc cgagatcgcg gctcagattc
      721 ccgacggtcg ttcgattgtg gtgccaaaag ccggtcactg cccgcagatt gagcaaccat
      781 cggtggtcaa ccacgtgctg gtggacttcc tgagcggggt acaacgaaag gtgccggcct
      841 catggtcaac gaactgacgg acaaggtcgc gatcgtcacc ggcggcgcgt cgggacttgg
      901 tgaaggactg gtgcgacggt tcgcggccga gggcgcgaag gtcttgatcg gcgatatcga
      961 cgtcgagcgc ggcaaggcgc tggcggcgga actcggccag aacgcgctgt tcgttgaagc
     1021 cgacgtcgct gacttcggcc aggtcggggg gctggtgtcg tcggcggtcg agcacttcgg
     1081 cgggctgcac gtgatggtca acaacgcggg agtttcgggc accatgcacc gtcgcttctt
     1141 cgacgacgac ctcgcggatt tccaccaggt gatggcgatc aacgtcgggg ccgtgatggc
     1201 ggggacgcgc gacgccgcac gccatatgtc caagcacggt ggcggatcga tcatcaacct
     1261 cacctcgatc ggtgggatcc aggccggcgg cggggtgatg acctaccgcg catcgaaggc
     1321 ggcggtaatc cagttcacca agtccgcggc aatcgagctg gcgcactacg agattcgtgt
     1381 caatgccatc gcgcccggca atatccgcac ggcgatcgta cggaaatcgg cggctgggga
     1441 ggacctggag aaactggagc agttcgaggc gaagatccgt cagcagatgc gtgacgaccg
     1501 tccgctcaag cgtgaaggta ccgtcgagga tgtcgccgag gcggcgctct acttcgccac
     1561 cgatcggtcg cgctatgtca ccggaaccgt attgccgatc gacggcggca ccgtcgccgg
     1621 caaggtgatc cctcgaaagt gacctgttta aatcagtcgc ttgacttaag acgcggaacg
     1681 gcgttgacta ggcaggtgac caccgccagg accgtgcgcg ccgatcgagc cagcagcacg
     1741 caagaggcaa tactgaaggc cgccgaacgg ctgtacgccg agcacggcat gttcgccgtg
     1801 tcgaacaggc aggtcagcga ggcggccggc cagggcaaca acgccgcggt cggctatcac
     1861 ttcggtacca agaccgatct ggtgcgcgca atcgagcaga agcaccgcgc gtcgatcgag
     1921 cggctgcttg agcggatggt ggccgaaacg ggcgactccg cggagttgcg ggattggatc
     1981 gcatgcctgg tgtgctcact caccgagcac ctggcccagc tcggaaaccc cacctggtac
     2041 gcgcgcttcg ccgcccaggc gctgacggac cccgcgtacc acaagatcgt catcaaggat
     2101 gcgctcgcat caccatcgct ggttcaggtc gtcgacggaa tcacccgctg cctgcccaac
     2161 ctgcccgcgg ccgtcgtgac ggagcgcaac atcatggcgc gcaacctgat gatgcacacg
     2221 tgcgctgact tcgagcgagc ctacgccgaa ggcgccgacg tcccccgaac cagttgggag
     2281 gcggtcggtt ctggcctgat cgacgcgatc gtcggactct ggctggcacc cgtcacggag
     2341 catccgtgac atgaaagtga ctgtcgacca agacaaatgc gtgtcgtcgg gccagtgcgt
     2401 cctgaacgcc gccgagatat tcgaccagcg cgacgacgac ggagtcgttg tcctactcaa
     2461 cgaaaacccg cccccaccgc aagcagagaa cgcccgcaag gccgcggcgg cgtgcccggc
     2521 cctagccatc gacatcgagg aatgagaaag actccatgtc cgacaccttg accggaaccg
     2581 caaccgaggt acccgactat ccgatggatc gggacgcccg ctgccccttt gcgccgccgc
     2641 cgcaggtact cgagatgggc gaggcgaagc cactttcccg cgttcggatt tgggacggca
     2701 gcacaccttg gctgatcacg ggtcatgcgg tggcccggga gttgttcgcc gactctcgcg
     2761 ttagtgtcga cgaccggcag gatggcttcc cgcactggaa cgagcacatg ctctcaacgg
     2821 tgcacaagcg gccgaggtcg gtattcacct cggatgccga ggagcacacc cgcttccgcc
     2881 ggatgctgtc caagccgttc accttcaaac gcgtcgaggg cctgcgcccc gcgatccaag
     2941 aggtgaccga cgagtgcatc gacgcgatcc tggccggccc acagcccgcc gacatcgtct
     3001 cgaagctcgc cctaccggtt cccacccgcg tgatcagcga aatgctcggt gtgccatacg
     3061 aagaccacga gttctttcag caccatgcca acgtggggct cgcccgctac gccaccgcgg
     3121 aggacggcca aaagggcgcc atgggcttgg cccagtacct gatcgacctc gtcaagaaga
     3181 agatggagaa cccgtccgag gatgcggtgt cggatctggc cgaacgtgtc aacgccggcg
     3241 agatcagcgt caaggaagcc gcacagctgg gcaccgggct gttgatcgcc ggccacgaga
     3301 ccaccgccaa catgatcggt atcggcgtgc tggccctgct ggagaacccc gaacaggctg
     3361 tgctgcttcg tgattccgac gacccgaagt tcatcgcgaa cgcggtcgaa gaattgatgc
     3421 gctatctgtc gatcatccag aacggtcagc gccgggtggc catcgaagac atcgagatcg
     3481 ccggcgagac catccgcgcc ggcgagggca tcgtcttgga cctggcaccg gcgaactggg
     3541 atgcgactgc atatccggat cccgacaagc tcgatctcac ccgcgacgcc ggtcagcagc
     3601 tgggcttcgg ctacggccgc catcagtgcg tcggacagca gcttgcgcgc gccgagcttc
     3661 agatcgtgtt ccacacgctg ctgcgtcgca ttccgacgct gcgccttgcc gtgccgctcg
     3721 acgaggttcc cttcaagcac gaccgcctcg cctacggcgt atacgaactt cccgttacct
     3781 ggtgaatgga gtgtcaacga tgtcgacttc tgcttccacg ctctacccgc ccggaggcta
     3841 cggtgcgccg aaggaccggc gcggacacgc gggcgcgcct gtcgggcttc cggcgggaac
     3901 cgaagtgttc tcagcggaca accacatttc cgtggccgac gacatcttct acgagcgctt
     3961 tcccgacgac ctgaaggaca aggcgccgcg aatctggtac gaggacggcg cgtacatggt
     4021 cggcatggcc aagggaaaga ccttcctgcc gggcgatttc agccgggtgc tcatgcagta
     4081 cgacgacctg gcgggcgcat ccacgaacaa catcgaggcg cgcgtgcgtg agctggcgga
     4141 ggacggcgtc gacaaggagc tggcgttccc caacgccgtc ttggcactgt tccactatcc
     4201 cgacaaggac cttcgcgagc gcgttttccg gatctacaac gagtacatgg ccgaccttca
     4261 agagcgcagt aacggccact tctacggagc cggcctgatc aactggtggg atcccaaagg
     4321 cgcacggcgg acgctgaccg agctgaaggc actgggactc aagacgttcc tgatgccgat
     4381 caatcccggc aaggacgacg gcgacaaggt gatcgactac ggcagcaccg taatgagcgc
     4441 ggtgtgggac gagatcgagg aagccggact gccggtgagc caccatatcg gggaaactcc
     4501 accgaagacg ccgtgcgagt tcaacagcgt cgtcgtcggc atgatgatca acatcgacgg
     4561 gttccgcgag gtgttctcca agtacatctt cagcggcatc ctcgatcgcc atccgaagct
     4621 gcggatcggc tggttcgagg gcggaatcgc ctgggtgccg tgggctctgc aggacgccga
     4681 gcatctgctg gcgtcgtatc agcacatgtt caaccacaac ctcgagcacg acatccagta
     4741 ctactgggac acccacatga gtgcgtcgtt catggtcgac ccgctgggcc ttcaattgat
     4801 cgaccggatc ggcgtcgaca aggtgttctg gtcgtcggac tatccgcaca acgagagcac
     4861 gttcggctat tcggagaagt cgctggcgtc tgtcgtcgag gctgtcgggc ccgacaacgc
     4921 cgccgagatc gtcagcggaa acatcaagaa cttccttggt atcgaatgac cgccttcgcg
     4981 acggcggtca catccgccca cgacatcccg gaccttcccg accgggcgcg catgtatcgc
     5041 gaaagcggcg cacggctccg gtcggccatg gcggacaacg gtattgatgc gttgatcctg
     5101 ctcggaaatg gcaacgtcgt atatgccacg ggagcgagct ggccgctatt ggatgccgga
     5161 ctgtcccacg tcgagcggcc ggtcgcgatc gtcctggccg acgacgagca tccgcatctg
     5221 ttcatgccgt tccgggaagg cgcaccgtgg gagtcgcaga tccccgccga ccatctgcac
     5281 ccgccactct tcctcgaatt cgacgaaggc gtcgaccatt tcgcacgcgt actcggcgac
     5341 ctcgtcccgg cgagtgcgac gatcgccgtc gacgaattga ccggcgcgat gcgccgtgct
     5401 ggcgatcgac tgttccctgg cgggccaccg tcggacgcgg cacctgtggt cggcacggcg
     5461 aagctggtga agacggttga ccagatctcg tgcattcgaa gggcgtgccg catcaccgac
     5521 gaggcggccg tcgacgtcca gaaggccttg gcaccgggtg tccggcaaat cgatctgtcg
     5581 gcgcaatttg tccggcgcgc tttcgaactc ggcgcgacgg ccagcatgct cgaacccatc
     5641 tggcaggtca tgcccgccac gaaggccgag ggcacgtgga ccactcacgg ggacctcgcg
     5701 cttccgctgt tgactacgga gcgtgagttg gccaagggcg acgtgctgtg gacggacgtc
     5761 agcatcacct ataccgggtt ctgctcggac ttcggccgga cctggttggt cggtgagacg
     5821 ccgtctgcgc gccagcagga tcagttccgg cagtggcggc acatcctcga tgccgtgctg
     5881 aaggtaacca aggccggcgc gacgtccggc gacctggcgc gcgcggccat cgccgccaac
     5941 ggtggacaga agccgtggct gccgcacttc tatctgggcc acggcatcgg caccaacgcg
     6001 gccgaaatgc cgatgatcgg aacggatctc ggcgaggagt tcgacgacaa cttcgtgttc
     6061 ccggccggaa tggtgctggt gctcgagccg gtggtctggg aggacggcac cggcggctat
     6121 cgaagcgagg agattgtggt tattaccgac gacggccacg tgtcgttgac caactacccc
     6181 tatgccccct atggcgactg aagtcctgcc cgatgaccgg gcactgcgaa tcggccgtcg
     6241 cgaacgcgca ctggcacaga tggaggccca cgacctcgat gtgctcgtgc tcgggcgcca
     6301 ggccaatgtg cggtatgtga cgggtgcgcc ccagctttgg gtggcgggca cgcgcccgtt
     6361 cggccccatc tgcatggtcg tgaggtcgac gggcgagatc catctgaaca gcacctggga
     6421 tgagggcatg cccgacgaca tcccccacga ccgcctgtac gggttggcgt ggaacccgat
     6481 gacgctgatc gaagtcctca agagcatcga cggcgcgtcg actgcccggc gggtcgggac
     6541 cgacgctcta acaccgacgt tcgcaaagct gttgccgatg gcatttccga atgcggagct
     6601 cgtcgacgct gaaccggcga tgcgggcggc gcggcggatc aagacaccgg acgaggtggc
     6661 cgcgctgcgc ggggcgcttc gggtggccga gaagtcactg gccaccgcgg tcgccgaact
     6721 gcgggttggt gtcaccgctc aggagctgac gggaatcctg ctcgaggccg aggctgcagg
     6781 cggggtgagt acccccgcca cacaggacgg cgcatgggtc acgtcgcggg agcatccctg
     6841 gcgacgggct cgcagcgacg ggcgaatcga gaacggcgac ctggtggcat tctcagcggg
     6901 ggtgcttgcc gacggctacg tcggcgaggt gggccggacc tggcccgtcg gcgatgtcga
     6961 cgcatctgcg ctatacgacc gttggaatac gttgtgggac aggctcgctg cggcctgtca
     7021 gccgggacgg ccagctggcg atctgctcgc ggcgtacgag gccgccaacg agccgcttcc
     7081 gccgacgccg gtcgcgcacg gcctcgggct tggcttcgat ccgccggtgg tgacgccaga
     7141 cctgcgaatt accgctgccg aagagatcct tgagccgggg atggtgctgt ctgtcaccgg
     7201 gtacgtctgg caagagggcg tgggcgcggt gttcggcagg gaggcggtgc ttgtcacctc
     7261 cgaccggcct gaggtgctga cgtcaagtcc gtcgtggagt attcgcgccg gcgtcggaat
     7321 cggctgaggg agaaaacatg tcggacagtc gacccaagcc tgaggagctg atcctctacg
     7381 agaaggaccc gaagacaaag atcgccacga tcactttcaa ccgtcctgag tacctcaacg
     7441 caccgacgtc ggcagcgcgg ctgcggtacg ccgatctgtt gcgcgcggcc accgtcgaca
     7501 atgacgtcaa ggtggttgtg atccgcggcg tcggcgacaa cctcggcagc ggcgcggatc
     7561 tacccgaatt catggagggc aacgacaatc ccgagttacg gctggctgag ctgcgactgg
     7621 aggacgaagg ggtcggcgag gtcagctatc cgcccaaggg atcgttccgt cacggtgcga
     7681 cgatcagcgc ctggtatgcg aatgtgcagg cgggtaaccg gccgctgcag gagctcaaga
     7741 agatcagcat cgtcgaggcg aagggctatt gctacggctg gcacttctat cagtgtgccg
     7801 atgccgacct ggtgatctcg tccgacgatg cgctgttcgg acatccgtcg ttccggtatt
     7861 acgggtgggg cccgcggatg tggacctggg tgcagatgat gggcctgcgc aagttccagg
     7921 agatggtgtt caccggacgg ccgttcaccg ccgaggagat gtatcagtgc aacttcctca
     7981 acaaggtggt gccacgcgag gcgctcgagg ccgaggtcga caagtatgcg cgggcttgcg
     8041 cacgtaaccg ccccgtcgac accgtcttcc agcagaagct gttcttcgaa gtcttcaagc
     8101 agcaccaggg tgagtacatg ggcagcctgc tgtcggcgtt cttcgagtcg atgggcagtg
     8161 gcgtcgtcaa cgacgactcg gacgacctgg acatgtacga ggccatcgac agtggcctgt
     8221 ccgatgcggt caacgacaac gacagcaagt tcccgccgga cttccggctg agcaagtcga
     8281 accgcaagaa gaaggactag tgcaccccct ggacgggtac gtcgtcgttg acttgtccac
     8341 gggcatcgcc ggggcgtatt gcacgaagct actcgccgac ggcggagccg agatcgtcaa
     8401 aatcgaaccg ccggaagggg actcgcttcg gcggtggtcg gcgtccggtg ccgaaatccg
     8461 gcccggtgac gacggtgcgc tgttcagcta tctggcgtgc tcgaaacgca gcgtcgccgc
     8521 cgaccccgac gtcgacggcg acttcgtcaa cgcgttactc gccgatgccg acgcggtggt
     8581 gtggtcgcgg ggttcgacga tcgccgacgc cttcgcaccc gccgaaatcc accgcaacca
     8641 cccgcacttg accgttactt ccattacgcc ctttggcatc gagggtccct gggcggatcg
     8701 accggccacc gagttcacgc tgcaggcatg gtcaggcggc atcgtgggcc tcggccgcgg
     8761 atcggccgac cgcgcaccgg tattcgtcgg cggccaggtc ggcgactacc tcgccggggc
     8821 ctacgccagc gtggccacga tggcctcccg aatgcggggc gccgccgagc tcatcgatct
     8881 gtcgatgctt gaagcccaaa tcctcggcct cacctactat cccgtgacct attacgagat
     8941 gctcggccgg ccgtggcggg acgttcggcg gctgaccgtg ccgggcatcg cgcgggccaa
     9001 agacggactg atcgacatcg gctgcggcac cgcgcagcag tggttcgacc tatgcgcgat
     9061 gaccggtcat caggactgga tcgacgagga gtcgccgctg tcgatcactg agcaggccaa
     9121 cgagaatgcc gacgaactgt acgcgtgggt cgaaagccaa accgtcgacg agatccgcga
     9181 cctcgcaacg gcattccgga ttcccaacgt accggtcgcc aacggtgcca acatcgagtc
     9241 cctcgaccac ttccaggcac gcggctcgtt cattgccaat ccgcgcgacg gctttacgca
     9301 gccggggccg ccctatcgca tgcagccggc gcagcttcgt gcgccgcaac ccgcaccgcg
     9361 cctcggcgaa cacaccgacc agcaccgtca gcgaggtcga cgcaatcggg acacactgcc
     9421 aacaatgccg tcgttcgagg ggctacgcgt ggtcgacatg acgacgttct gggccggccc
     9481 gtcgtgcacg cacatgctgg ccatgctcgg cgccgaggtc atccacgtgg agtcgacgcg
     9541 acggcccgac ggcacgcggt tgatcgcggg catcccgatc actgaagacc agtggtggga
     9601 gaagtcgccg atcttctcgg gtctcaacac gaacaagaag gggctgacac tcaacctgca
     9661 gagcgacgca ggacgcgaag tcctcatgca attgatcgcg acgtgcgacg tcatcgtcga
     9721 gaacttcacg ccgcgggtcg tcgaccagat cggcctggat ttcgccgcgg tgcaagcggt
     9781 ccggccggat gcgatcatgc tgcggatgcc gggcttcggc ctcgacggac cgtggcggga
     9841 caattccgca ttcgcgtatg tcatcgaggc ggcgtccggg gtcagctggc tgaccggcta
     9901 tccggaccgc aacccgtatg agccgtattc ggtcggcgac cccaacgccg gcgtgcatgc
     9961 gctcaacgca ttgctgctcg ccctcgagca tcgtcgccgc accggagagg gcgtgctcat
    10021 cgaggcggcc atggtcgacg ccgcgctcaa catcgccgcc gaacaggtca tcgagtactc
    10081 ggcctacggc gcgctattgc ggcgcgacgg caatcggggg ccgacggcgg caccgcaaaa
    10141 cctttaccgc accaacgaga tcgacgagtt cggtcgtcgg gactgctggg ttgccgtggc
    10201 cgtcgccacc gacgagcagt gggcaggcct ctgcactgcg gtggataaac ccgcatgggc
    10261 gacggagatc cccacagccg aagcacgacg tgcgcaggaa gacatcatcg acgagcacct
    10321 tgcggcgtgg tgcgggcagc gcaccggtga cgacattgtc gaggccctgt ggagccacga
    10381 cgtgcctgtg gccaaggtca tgcagccgca ccgccaatcc gaactgccac agctcgccgc
    10441 ccgtggcttc ttcgaagatg tcgaccatcc tgtgaacgcc acaacactgc acagcacgct
    10501 gccgttcacg tcgacgcgcg gcgccgaccg catccatacc cagcctgccc cgctgctcgg
    10561 acagcacaac cgcgagattc tgatcgaact ggggctgagc gacaaggaca tcgccgaact
    10621 ggagaccgag ggcgtgatcg gcaccgcacc agccatgtac ggcacgaagg tctgatagct
    10681 tccaggcgat ggccatcgac ccgtcccaca tcctgctcac cggccgcgtc gcggtggtga
    10741 ccggtggggg agggggcatc ggccggggta tcgccgaagg gctcgccgcg ttcggtgcat
    10801 cagtcacgat ctgggagcag aacgccgaga cctgcacctc ggccgcggaa gccattggcg
    10861 cccttggcat tgtgaccgat gtgcggaact ccgagcaggt cgatgcggca ttgcagcgca
    10921 ccacaaccga actcgggatc ccgtccatcc tggtcaacaa cgcgggcggc gtcttcaagg
    10981 ccccgcttct cgacaccacc gagaacggct gggatgcgct gtacaagagc aaccttcgcc
    11041 atgtgctgct
//
LOCUS       DASRJA010000578         4423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2004301,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000578 DASRJA010000000
VERSION     DASRJA010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004301"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFC01_25480"
     CDS             complement(<1..209)
                     /locus_tag="VFC01_25480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83001.1"
                     /translation="MSDSDSNDTSGATQPEPQPRAEPEPEPQPRAEPEPEPQPQRTRS
                     GKGVRAIGVIVVIAGVILAAAGVVT"
     gene            467..1948
                     /locus_tag="VFC01_25485"
     CDS             467..1948
                     /locus_tag="VFC01_25485"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN83002.1"
                     /translation="MDTTVATREANQSYRDVLRNRQVAGLLLGDLLANVGTGMIIVAM
                     PVQTLSIHGSVPPAIAIGLVEAAPFILSTILALTIGLGRLRVSPRTLLVADCVLQSLI
                     FATLGVLAVTEQLTLPVLIVGLLLGATFRVAGSSSRRLLATSVTGETGQVAVNGLLGL
                     NATFALYIVGPVLGGVIVASTSAGVALFFDACGAVILMGAVLISVPRAVDDRDALRRP
                     RARAGDSGWRVLRRRPVAARLLVVVFFFNFFYMPIEVALPMYVRGSLHADASELGLMW
                     GALGVGAFAGAALVNQLRNLPQRQVLIAIIGLWAMCPIALAFAGSLTLALIVFGLGGL
                     VWAPFTPVAYSFLQSGLKPDEQQQVVTLWTTGATVAAPLGLIVGGPLIALTGSTGGLL
                     LSGGLTLLLLPIAALAVLRRAPGRSHISRAIRRAITAVQPLPGHQTRNEEWGETPAAL
                     GSLGATWHDFEGDQQDRPGRAAARPRAAAPRPRPDRPGVRAAA"
     gene            1851..2300
                     /locus_tag="VFC01_25490"
     CDS             1851..2300
                     /locus_tag="VFC01_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012690892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN83003.1"
                     /translation="MTSRTVPAEQLRDLARLRRVRDRIDREYAQPLDVEALARAVHMS
                     AGHLSREFRLAYGESPYAYLMTRRIERAMALLRRGDLSVTEVCFEVGCASLGTFSSRF
                     TELVGVPPSTYRREQAEATAGMPSCVAKQVTRPIRNREAPANEPQLA"
     gene            2306..2716
                     /locus_tag="VFC01_25495"
     CDS             2306..2716
                     /locus_tag="VFC01_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN83004.1"
                     /translation="MDITINASFLPHDDPDAALAFYRDALGFEVRNDVGYGGKRWITV
                     GPADQPGTSIVLHPLGAYPGITDDERRTIAEMMAKGTYATILLATKDVDGTFERVQAT
                     DAEVVQEPTDQPYGVRDCAFRDPAGNMVRIQQVP"
     gene            2829..>4423
                     /locus_tag="VFC01_25500"
     CDS             2829..>4423
                     /locus_tag="VFC01_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZN83005.1"
                     /translation="MSTATRVDTQSPAQHLADSHDLIRVHGARVNNLKDLSVEIPKRR
                     LTVFTGVSGSGKSSLVFGTIAAESQRLINETYSAFVQGFMPTLARPEVDVLEGLTTAI
                     IVDQERMGANARSTVGTATDANAMLRILFSRLGQPHIGPATAFSFNVPTRTASGAMNV
                     DKGAGERIVVRNVVYLGGMCPRCEGMGSVTDFDLSQLYDDRLSLSEGALKVPGYSMDG
                     WYGRIFSGSGLFDMDKPIGKYTKKELHDLLYKEPTKIKVEGINLTYEGVITKIQKSML
                     SKDVDTLQPHVRAFVQRVVTFTACPECDGTRLSKEARSSKIAGKNIADVCAMQISDLA
                     TWVRDLDEPTVAPLLGALGDTLDSFVEIGLGYLSLDRPSGTLSGGEAQRTKMIRHLGS
                     SLTDVTYVFDEPTIGMHPHDIQRMNDLLLQLRDKGNTVLVVEHKPEAIAIADHVVDLG
                     PGAGTGGGEVVFEGSVEGLRACGTLTGRHLDDRAALKPSARTPSGVLEVRGASTNNLQ
                     DVDVDIPLGVLVVVTGVAGSGKSS"
BASE COUNT          710 a         1498 c         1465 g          750 t
ORIGIN      
        1 taggtgacta cgccggcggc cgcgaggata actcctgcga tcaccacgat cacgccgatg
       61 gcacggacgc ccttaccgga ccgggtgcgc tgcggctggg gctcgggctc gggctcggcc
      121 cgcggctggg gctcgggctc gggctcggcc cgtggctggg gctcgggctg ggtggcgccg
      181 ctggtgtcgt tgctgtcgct gtcggacatg tgcaccttcc tagctcgtct gcgcagactc
      241 taggcgtgcg tcgggtgtcc cgaggcacga tccaccagga cacgccggtc accaagccca
      301 tgacggggtc cgcaccgtgc acagtcacgt tgccgccgcg cggaggaagt tgggaacccg
      361 gacccgcgag gaagcggtcg cgcgctggtg tgctcggcat cgtcgatgtc agaccggtca
      421 ggaatcgaga agcgccggcc gccgtcgcaa acctagcgtg accgtcatgg acaccaccgt
      481 ggcgactcgt gaagccaacc agtcctaccg cgacgtgttg cgcaaccggc aggtcgccgg
      541 cctcctgctg ggtgatctcc tggctaacgt cggcaccggc atgatcatcg tggccatgcc
      601 ggtgcagacg ctgtccatcc acggcagcgt gccgccggcg atcgctattg ggctggtgga
      661 ggccgcgccg ttcatcctgt ccaccatcct tgcgctgacc atcgggttgg gacggctgcg
      721 agtgtcgccg cgaacccttc tggttgccga ttgcgtgttg caatcgctga tcttcgccac
      781 cctcggcgtc ttggccgtca ccgaacaact cacgctgccc gtgctgatcg tcggcctgct
      841 cttgggcgcg acattccggg tagccgggtc aagcagccga cgcctgctgg ctacgtcggt
      901 caccggcgag accgggcagg tcgccgtcaa cggtctactc gggctgaatg ccacgtttgc
      961 gctctacatt gtcgggcccg ttttgggcgg ggtgatcgtg gcatccacca gcgcaggcgt
     1021 cgcgctcttc ttcgacgcgt gcggtgcggt gatcttgatg ggcgccgtcc tgatctccgt
     1081 tccccgagca gtcgacgacc gggacgcgct tcgtcgcccg cgagcccgag ccggcgactc
     1141 gggctggcgg gtcctgcgtc gccgcccggt cgcggcccga ctgctggtcg tcgtgttctt
     1201 cttcaatttc ttctacatgc cgatcgaggt ggcgcttccg atgtatgtgc gcgggagcct
     1261 gcacgccgac gcgtctgagc tgggtctgat gtggggcgcg cttggggttg gcgcattcgc
     1321 cggtgccgcc ctggtgaatc agctgcggaa ccttccgcag cgccaggtac taatcgcgat
     1381 catcggcttg tgggcgatgt gcccgatcgc gcttgcgttc gccggcagcc tcactcttgc
     1441 gctgatcgtc tttggtctgg gcggcctcgt ctgggcgccg tttacgccgg tcgcgtacag
     1501 cttcctccaa tcggggctca agcccgacga acaacagcag gtcgtcacgc tctggacgac
     1561 cggcgctaca gttgcggccc cgcttggcct gatcgttggc ggaccactga tcgcgctgac
     1621 cgggagcacc ggagggctcc tgttgtccgg tgggctaacc ctgctgctgc tcccgattgc
     1681 ggcgctggct gtgctcaggc gcgcacccgg gcggtcgcac atttcgcgcg cgatacgtcg
     1741 ggcgataaca gctgttcaac cgctccccgg ccaccaaacc cgcaacgagg agtggggcga
     1801 gacgccagcg gcgctgggca gcctcggtgc gacgtggcac gatttcgaag gtgaccagca
     1861 ggaccgtccc ggccgagcag ctgcgcgacc tcgcgcggct gcgccgcgtc cgcgaccgga
     1921 tcgaccggga gtacgcgcag ccgcttgacg tcgaggcgct ggcccgggct gtacacatgt
     1981 cggccgggca cctcagccgc gagttccggc tcgcttacgg cgaatctcca tacgcctatc
     2041 tgatgacgcg gcgcatcgag cgcgcgatgg cgttgctgcg ccgcggtgac ctcagcgtca
     2101 ccgaggtctg tttcgaggtc ggctgcgctt cgctcggcac cttcagcagt cgctttaccg
     2161 agttggtcgg cgtaccaccc agcacctacc ggcgcgagca ggcggaggcg acggcgggga
     2221 tgccgtcctg cgtggcgaaa caagtgacca gaccgatcag gaatcgagaa gcaccggcca
     2281 acgagccaca gctagcgtga tcgccatgga tatcaccatc aacgcaagct tccttccgca
     2341 tgacgacccg gacgccgccc tggccttcta tcgcgacgcc ctaggcttcg aggtccgcaa
     2401 cgacgtcgga tacggcggga agcgatggat caccgtcggc cccgccgacc aacccggcac
     2461 gtccatcgtt ctgcacccgc tgggcgccta cccgggcatc accgacgacg agcgccggac
     2521 catcgccgag atgatggcca agggcaccta cgccacgatc ctgctagcca ccaaggacgt
     2581 cgatggcacc ttcgagcggg tacaggctac cgacgctgag gtcgtccagg aaccgaccga
     2641 ccagccgtac ggggttcgcg actgcgcatt ccgcgacccc gcgggcaaca tggtccgcat
     2701 ccagcaagtg ccctgagccg cccgcggtca ggctccgcga cctctagtcg ctcggcctgc
     2761 ccactggaca cgttccaacg ctcgatcccg cacgccccag gtttctgccc aacagagatg
     2821 gagacccgat gagcaccgcc acgagggtgg acacgcagtc ccccgcgcag caccttgccg
     2881 acagccacga tctgatccgg gtgcacggcg cgcgcgtgaa caacctcaag gacctcagcg
     2941 tcgagattcc gaagcgtcgg ctgacggtgt tcaccggcgt ctccggctcc ggcaagagct
     3001 cgctggtgtt cggcaccatc gccgctgagt cgcagcggct gatcaacgaa acctacagcg
     3061 ccttcgtgca gggcttcatg ccaacgctgg cccggcccga ggtcgacgta ctcgaggggc
     3121 tgacgaccgc gatcatcgtc gaccaggaac gaatgggtgc caacgcccgc tccacggtcg
     3181 gtaccgccac agacgccaac gccatgctgc gcatcctgtt cagccggctc gggcagccgc
     3241 acatcggccc ggctactgcc ttctcgttca acgtcccgac gcggacggcg agcggggcga
     3301 tgaacgtcga caagggagcg ggcgagcgga tcgtcgtacg caatgtcgtc tacctgggcg
     3361 gcatgtgccc acgctgcgag ggaatgggct cggtcaccga cttcgacctg tcgcagctgt
     3421 acgacgaccg cctgtcgctc agcgagggcg cgctcaaggt ccccggctac tcgatggatg
     3481 gctggtacgg ccgcattttc agtggctcgg gcttgttcga catggacaag ccgattggca
     3541 agtacaccaa aaaggagctg cacgacctgc tctacaagga gcccaccaag atcaaggtcg
     3601 agggaatcaa cctgacctac gagggtgtga tcacgaagat ccagaagtcg atgctgtcca
     3661 aagatgtcga cacgctgcag ccacacgtcc gcgctttcgt gcagcgggtg gtgacgttca
     3721 cggcgtgccc cgagtgtgac ggcacccggc tcagcaagga ggcccgatcc tcgaagatcg
     3781 cggggaagaa catcgccgac gtctgcgcca tgcagatcag tgacctggcc acctgggtgc
     3841 gtgacctcga cgagccaacg gtggcgccgc tgcttggcgc gctgggggac actctggact
     3901 cgtttgtcga gatcgggctc ggctatctgt cgctcgaccg gccgtccggc acgctgtcgg
     3961 gcggcgaggc acagcgcacc aagatgattc gccatctcgg gtcgtcgctc accgacgtca
     4021 cctacgtctt cgacgagccg acgatcggta tgcacccgca cgacatccag cggatgaacg
     4081 atctactgct gcagttgcgc gacaagggaa acacggtcct cgtcgtcgag cacaagccgg
     4141 aggcgatcgc catcgccgac cacgtcgtcg acctcggccc cggtgccggc accggcggtg
     4201 gcgaggtggt gttcgagggc agcgtcgagg ggctacgcgc ctgcggcacg ctgaccgggc
     4261 ggcacctgga cgaccgcgcc gcactgaagc cgtcggcacg gactccgtcc ggagtgctgg
     4321 aggtgcgcgg cgccagcacg aacaatctgc aggacgtcga tgtcgacatc ccgcttggcg
     4381 tgctggtggt ggtgaccgga gtcgccggct ctggcaagag ctc
//
LOCUS       DASRJA010000579        14398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_198754,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000579 DASRJA010000000
VERSION     DASRJA010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14398
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198754"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..529)
                     /locus_tag="VFC01_25505"
                     /pseudo
     CDS             complement(1..529)
                     /locus_tag="VFC01_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417215.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            688..1557
                     /locus_tag="VFC01_25510"
     CDS             688..1557
                     /locus_tag="VFC01_25510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83006.1"
                     /translation="MERLLNYPPSGPPSVRGPGYQPGPQRRTTWLWISLAIAVIIIVV
                     LAAVVITQLTVDKSNKSTPAAPQPSATQSAPPPSGTQQRPNGGAGQTMTCEGYTASVD
                     AGSQPGWQPTVGKRGLAYAVPPDWTVAKCGAQMGWQKPCPDGQQGACVIRAFSAVSTL
                     ADPSCDNGNLAKAGVAGSKNPDIKAALEDESKTVPIIYTSNSGQAPKVDFGPVREFTI
                     GAHPAVQMVATVTDIAGDACTGPSALHSMVVTTVPNVEGSVVFLISLRQGAQGAPNPN
                     VINEMVKTLRSPA"
     gene            complement(1610..2443)
                     /locus_tag="VFC01_25515"
     CDS             complement(1610..2443)
                     /locus_tag="VFC01_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZN83007.1"
                     /translation="MRDGLGPARVRLRGGLVVDEFRARWGVSAAAKVLAGEVVLPDGT
                     VVTDRTVLPAGAHVYFYRELRDEVPVPFDIPVLYRDDDIIVVDKPHFLATMPRGRHVA
                     QTALVRLRRELDLPELSPAHRLDRLTAGVLLFTTRRELRGRYQTLFSCGLVRKTYLAR
                     AAVDPDLRLPRVVRNRIVKRRGQLQAVEEPGEPNAETLVEHLGEGLYRLTPRTGRTHQ
                     LRVHMASLGVPIVGDPLYPEVVDVAPDDFSQPLQLLAHSLECEDPVNGRRCEFVSRRT
                     L"
     gene            complement(2464..4209)
                     /locus_tag="VFC01_25520"
     CDS             complement(2464..4209)
                     /locus_tag="VFC01_25520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HZN83008.1"
                     /translation="MSDPNPAPGKGQTFLGPHQREQAWEQLGSEQFDVVVIGGGVVGA
                     GAALDAATRGLKVALVEARDFASGTSSRSSKMFHGGLRYLEQLEFGLVREALHERELS
                     LTTLAPHLVKPLPFLFPLTSRWWERPYVAAGILLYDQLGGAKSVPAQKHLTRAGALRL
                     CPGLKRSSLIGGIRYYDTVVDDARHTMTVARTAAQYGAVVRSSSQVVSLLREGDRVTG
                     VTIRDSEDGAITEVHGHVVVNATGVWTDEIQALSRQRGRFRVRASKGVHVVVPRDRIV
                     SEVAIILRTEKSVLFVIPWGTHWIIGTTDTDWNLDLAHPAATKADIDYILGHVNTVLA
                     TPLTHDDIDGVYAGLRPLLAGESEETSKLSREHAVAVPAPGLVAIAGGKYTTYRVMAA
                     DAIDAAAEYIPARVAPSITEKVPLMGADGYFALINQTESVGAHYGLHPYRVRHLLDRY
                     GSLIGEVLAVAEGRPELLTPITEAPVYLKMEAWYAAAAEGALHLEDILARRMRISIEY
                     PHRGMDCAREVAEVVAPVLGWSAEDIDREVATYIARVEAEVMSQAQPDDESADALRAA
                     APEARAEILEPVPLN"
     gene            complement(4324..5739)
                     /locus_tag="VFC01_25525"
     CDS             complement(4324..5739)
                     /locus_tag="VFC01_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HZN83009.1"
                     /translation="MATRIVIIGGGPAGYEAALVAAARGPEVADVTVVDSDGLGGACV
                     LWDCVPSKTFIASTGVRTELRRAPDLGYALEFEDAKISLSQINERVKTLAAAQSADIA
                     ERLRNEGVTLIAGRGELVDDIPGMAAHTVKITAHDGDVSTIKADVVLIATGASPRVLP
                     GAEPDGERILNWRQLYDLEKLPEHLVVVGSGVTGAEFVNAYTELGVKVTVVASRDQIL
                     PHEDSDAAAVLEDTLASRGVTLVKNARAEKVTRDGDGVLVTMTDGRTVDGSHALMTVG
                     SVPNTSGLGLEHVGIELGQGDYLKVDRVSRTSVPGIYAAGDCTGLMLLASVAAMQGRI
                     AMYHALGEGLEPIRLRTVAAAVFTRPEIAAVGVPQSKIDDGSVPARTVMLPLNTNARA
                     KMSGLRRGFVKIFCRPATGVVIGGVVVAPIASELIMPIALAVQNGNDVEDLAQTFSVY
                     PSLAGSITEAGRRLMAHDDLD"
     gene            5805..6278
                     /locus_tag="VFC01_25530"
     CDS             5805..6278
                     /locus_tag="VFC01_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HZN83010.1"
                     /translation="MPLYAAYGSNMHPEQMLERAPHSPMAGTGWLHGWRLTFGGEDIG
                     WDGALATVVQDPASKVFVVLYDMTKEDEKNLDRWEGSELGIHKKIRCRVDRTSSDTSF
                     EPVLAWLYVVDAWEGGLPSARYLGVMADAAEIAGAPSEYVHGLRTRPARNIGPGT"
     gene            complement(6345..7232)
                     /locus_tag="VFC01_25535"
     CDS             complement(6345..7232)
                     /locus_tag="VFC01_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83011.1"
                     /translation="MQPELEALFDSQGGVASSGQVLNYLTRRGFESAVNSGALERIWP
                     GIYCRGEPDDFTRLRGLDFSCNAQVPVCLGTAAALHGFDTEEPADLHVLSPPGSRLRS
                     ADGLVVHRRDGAPLVKVGERLATSPAWTAVEVARGLRRPRALATLDAALRGGTSQRVD
                     LWRAAAQQSGRRGIVAVRNLIPLADGLAESPMESEARLAMIDGGLPTPVLQYEIIDGN
                     GELRRVDFAWPDQRVAVEYDGMDWHSGPEAMRNDRRRQAALQDVRWTVIPIVFEDVRY
                     RAWEFVARIDRQLQRARAA"
     gene            complement(7394..8563)
                     /locus_tag="VFC01_25540"
     CDS             complement(7394..8563)
                     /locus_tag="VFC01_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZN83012.1"
                     /translation="MSVADTTELWLDAHYDDLIAWRRHIHAHPELGRQEFATTQFVAS
                     HLADAGLNPKVLPGGTGLTCDFGPEHGPRIALRADMDALPMAERTGAPYASVVPNVTH
                     ACGHDAHTAVLLGAALALASVPELPVGVRLIFQAAEELMPGGAIDAISAGALTGVSRI
                     FALHCDPRLAVGKVAVRPGPITSAADQLEVTLQSHGGHTSRPHLTGDLVYGLGSLITG
                     VPGVLSRRIDPRNGTVMVWGAVNAGVAANAIPQTGTLAGTIRTASRDTWDSLESIVRE
                     IVSSVLQPLGIEHSLQYRRGVPPVVNEEISTRILTHAIEAIGTDVLCDTRQSGGGEDF
                     SWYLEEVPGAMARLGVWSGRGPQLDLHQPTFDLDERSLAVGVRALVNIIDQAAQF"
     gene            complement(8560..9741)
                     /locus_tag="VFC01_25545"
     CDS             complement(8560..9741)
                     /locus_tag="VFC01_25545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZN83013.1"
                     /translation="MPTATASISVEDAVNRRRGDLIELSHSIHAEPELAFAEHRSCAK
                     AQALVAEYGFEMTAAPGGLDTAFRADYGSGPLVVGICAEYDALPVIGHACGHNIIAAS
                     AVGTALALAEVADELGLTVALIGTPAEEAGGGKALLLKAGTFDDIAATVMLHPGPLDI
                     AAARSLALSEVTVRYQGRESHAAVAPYLGVNAADAVTVAQVAIGLLRQHLAPGQMVHG
                     IVTDGGQATNVIPGHAELRYTMRAVNSESLHELEDKMAGCFAAGSVGTGCTHTVAETA
                     PAYAELAPDPWLASVFRGEMVRLGRSPVAEDVEGTLPLGSTDMGNVTQVMPGIHPIVG
                     IEAGGASLHQPAFTAAAVEPSADKAVVEGAIMLARTVVRLAETDMERERVLELQARRA
                     S"
     gene            9798..10592
                     /locus_tag="VFC01_25550"
     CDS             9798..10592
                     /locus_tag="VFC01_25550"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HZN83014.1"
                     /translation="MTEPDAVAAESARAIRDRTGVDEHDVAVVLGSGWAPAVAELGDP
                     VAVVPMAELAGFTPPTAEGHGGQVLSLHVGDHRVLVFVGRIHAYEGHELRDVVHPVRA
                     ACAAGVRTVVLTNAAGGLRDDYTVGQPVLISDHLNLTARSPLVGAQFVDLTDAYSPRL
                     RTLAREIDPSLAEGVYAGLPGPHYETPAEIRMLRTLGADLVGMSTVHETIAARAAGAQ
                     VLAVSLVTNLAAGMTGQPLSHDEVLEAGRQSATRMGSLLGSVIARV"
     gene            complement(10538..11641)
                     /locus_tag="VFC01_25555"
     CDS             complement(10538..11641)
                     /locus_tag="VFC01_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HZN83015.1"
                     /translation="MLRWALLIAVTVVVTLPLTRVGVPSAALFAALVVGVALALASLA
                     PARVPRKAGIAAQGVLGVYIGTMVHEDAVGALGADWPIVIAIAVATLLLSIVAGALLG
                     LHRNVTPLTGSLALVAGGASGLVAIARELGGDDRVVSVVQYLRVGLVTASMPVVVTLI
                     YHADKSHGTAPVVETDSAPWYLSVAMLIALVLVGATVGKLIRLPGAGLLGPLALTVAL
                     EVTGLSFGLAVPMVLVQAGYMLIGWQAGLAFTRESLRAIGRMLPAAIALIVALGVATA
                     GLGVVLAHFTGLTPLEGYLATSPGGVYAVLATAVETGSNVTFIIAAQVVRILLMLFAA
                     PLMARAVVWMSERFGRQTRAITEPSREPIRVAD"
     gene            11749..12201
                     /locus_tag="VFC01_25560"
     CDS             11749..12201
                     /locus_tag="VFC01_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN83016.1"
                     /translation="MNRVDSPATELRESMMAATRQMRRHRPEHGLTLTQMELLGEVSR
                     TGVTTPAELANRLHVRVQSLTDSINELVSRGLTARRPDETDRRRQLIELTDAGTALLE
                     ADRAERDAWLHATMRDNLTPLEFDLLMLVAPVLRKLAYSDAKAGTLAP"
     gene            12198..13835
                     /locus_tag="VFC01_25565"
     CDS             12198..13835
                     /locus_tag="VFC01_25565"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HZN83017.1"
                     /translation="MTSSRTGAPAQEWISHDPDPQTAAELAALSEEELDERFAQPLTF
                     GTAGLRGPLRGGPNGMNLAVVLRTTWAVAKVLKDRGLAGKPVMVGRDTRHGSEDFALA
                     AAEVLAAQGFAVTLMFVAVPTPVLAFAVRHDRAAAGIQITASHNPPSDNGYKVYFEGG
                     YPIVPPIDHEIERAVAEAPYADEIPRRSVKASGVDAIQHYVDRAAHVRHTHGSVRVAL
                     TPLHGVGGEFAMDALAAAGIPDVHVVESQFAPDPDFPTVQLPNPEEPGAVDALLELAA
                     DVDAEIAIALDPDADRCAVGIPTPDGWRMLSGDETGWLLGDYILSQIEPGPVSEATLV
                     ASSVVSSRMLAAIANDHGAQHVETLTGFKWLSRADVGRPGCTLVYAYEEAIGHCVDPG
                     AVRDKDGISAMVLACDLVAALRNQGRTVTGALDDLALRHGVHTTTAVSRRVADGGEAE
                     ALMARLRATPPDRLAGFEITATDLLHAPTHLRTDALVFSGGDGGNWIRMVVRPSGTEP
                     KLKSYIEVHCANLGNIEATRTKARTVQDELVSVAQQF"
     gene            complement(13832..>14398)
                     /gene="upp"
                     /locus_tag="VFC01_25570"
     CDS             complement(13832..>14398)
                     /gene="upp"
                     /locus_tag="VFC01_25570"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HZN83018.1"
                     /translation="DERTDNAAFRAALRDLTLMLVYEATRDAACETVPVRTPLADTTG
                     CRLANPPLLVPVLRAGLGMVDQAHALIPEARVGFVGVARDEETHEPTPYLESLPDDLS
                     TQPVMVLDPMLATGGSMAHTIGLLHSRNAVDITAVCVVCAPEGIAALEKVAPNLRLFT
                     ATIDERLNDIAYIVPGLGDAGDRQFGPR"
BASE COUNT         2456 a         4975 c         4675 g         2292 t
ORIGIN      
        1 acagcggatc cctgacgatc ggcagccccc agcgatgccg tgcgtatacg cagctgatgc
       61 gccgtaagac ggtagaggcc gtcgtcggac acgagttcga tgtgtatctc cgcgttcggt
      121 tcacccggct cctcaacggc ctggaaattc ccacgccgct tcacaatccg gctacgcacc
      181 accgtcggca gctcgacgcc cgggttgacg ggccgcccgc gccagttacg tcttgcacca
      241 gcccttcggc gaacaaggtc tgataccggc cacggcacct cacgccgtgc cgtgccgtga
      301 acaacagcac gcccgcgttc agccgatcca gcctattggc gggcgaaagc tccggtagag
      361 cgagctcgcg gcgcaaccgg accagcgccg tctgcgcgac gtgccgcccc cgcggcatgg
      421 tcgccagaaa gtggcgtttg tcgaccacga cgatgtcgtc gtcgcgatac aacatcggta
      481 tgtcgaacgg caccgtcacc tcatcgcgca gctcgcgata taggtacaca tgcgcgcacg
      541 catgcctgac acaggattca tgcctgacac aggattgaag catcaatcct gtgaaatccg
      601 ggagccgaag cacatttcgg tcccgcacag gcagttgtgc ggataatgaa gcgacggacc
      661 ctgtgtggcc cgcaacttcc gaccccgatg gagcgtctgt tgaactatcc gccgtcaggt
      721 ccgccttcgg tccgcggccc cgggtatcaa cccggcccgc aacgccgcac aacttggttg
      781 tggatctcac tcgcgattgc ggtcatcatc atcgtggtct tggccgcagt ggtgatcacg
      841 cagctgacag ttgacaagtc caacaagtcc accccggccg cgccacaacc gtccgctacc
      901 cagtcggcac caccgccgag tggcactcaa caacggccca atggcggtgc ggggcagacg
      961 atgacctgcg agggttacac cgcttcagtc gacgccggat cacagcctgg ctggcaaccg
     1021 acggttggca agcgtggcct ggcctatgcc gtgccgccgg attggaccgt cgcgaaatgc
     1081 ggtgcccaga tgggctggca gaagccatgc ccggacggcc agcagggcgc ctgcgtgatc
     1141 cgtgcgttca gcgccgtgtc cacgctggcc gacccgtcgt gtgacaacgg aaacctcgcc
     1201 aaggctggcg ttgccggctc caagaatccc gacatcaagg ccgcgctgga ggacgaatcc
     1261 aaaactgtgc cgatcatcta cacctcgaac agcgggcagg cgcccaaagt cgatttcgga
     1321 ccggtgcgtg agttcacgat cggcgctcat cctgcggtgc aaatggtggc gaccgtgact
     1381 gacatcgcgg gggatgcctg caccggcccg tcggcgctgc acagtatggt agtcaccacg
     1441 gtgcccaacg tcgaggggtc cgtcgtgttc ctcatctcgc tgcggcaggg cgctcaaggc
     1501 gcgcccaatc ccaatgtgat caacgagatg gtcaagactc tgcgaagccc ggcctgacag
     1561 agcatttcag gcagggagcg cctcgtctgc ttaaacatcc ctcgggacgt cacagcgtgc
     1621 gtctgctgac aaattcgcat cgacgcccgt tcaccggatc ctcgcactcg agactgtgcg
     1681 ccagcaattg caatggctgc gagaagtcat ccggcgccac gtctactact tcggggtaca
     1741 gcggatcgcc cactatcggc acacccagcg acgccatgtg cacccgcagc tgatgcgttc
     1801 gtcccgtcct tggcgtcaac cggtacagcc cctcgcccag atgctccacc aacgtttccg
     1861 cgttcggctc gcccggttcc tccacggcct gtaactgtcc gcgccgcttg acgattcggt
     1921 tgcgtaccac cctcggcaac cgaagatccg gatcgacggc cgctcgcgcc agatacgtct
     1981 tgcgcacgag tccgcaagag aacaacgtct gataccggcc acgtagctca cggcgcgtcg
     2041 tgaacaacag cacacctgcc gtcaaccggt ccagtcgatg agccggcgaa agctccggta
     2101 agtccagttc ccgtcgcaac cgcaccagcg ccgtctgcgc tacgtgccgg ccacgcggca
     2161 tcgtcgccag gaaatgcggc ttgtcgacca cgatgatgtc gtcgtcgcga tagagcaccg
     2221 gaatgtcgaa cggcaccggc acctcgtcgc gcagctcgcg gtagaaatac acatgcgcgc
     2281 cggccggcag cactgtccga tcggtgacca ccgtgccatc cggtaagact acttctcccg
     2341 caagcacttt cgcggctgct gatacgcccc accgggcgcg gaactcatcg accaccagcc
     2401 caccgcgcaa ccgtactcgc gccgggccga ggccgtcgcg caccggtagc ggtgcggcac
     2461 gcctcaattg agcggtaccg gctcgaggat ctcggctcga gcctccggcg cggcggcacg
     2521 cagcgcatcc gccgactcgt cgtccggctg tgcctgggac atcacctcgg cttcgacccg
     2581 cgcaatgtat gtcgccactt cacggtcgat gtcctcggcg ctccaaccca gcacgggtgc
     2641 aaccacttcg gcgacctcac gagcgcagtc cataccgcgg tgcggatact cgatcgagat
     2701 cctcatccgc cgcgccagga tgtcctccag gtgcagtgca ccctccgcgg ccgcggcgta
     2761 ccacgcctcc atcttcagat acaccggcgc ttcggtgatc ggtgtcagca gctccggccg
     2821 gccctcggcc accgccagca cctcgccgat cagcgagccg tagcggtcca gcaggtggcg
     2881 cacccgatac ggatgcaggc cgtaatgcgc accgacgctt tcggtttggt tgatgagcgc
     2941 gaagtagccg tcggcgccca tcagcggaac cttctcggtg atcgacggcg ccactcgcgc
     3001 cgggatgtac tcggcggcag cgtcaatcgc gtcggcggcc atcacccggt acgtggtgta
     3061 cttgccgccg gcgatcgcga caaggccagg cgccgggact gcgaccgcgt gctcgcggga
     3121 cagcttggac gtttcctcgc tctcccccgc caacagcggg cgtagccccg cgtacacccc
     3181 gtcgatgtcg tcatgggtca acggcgtggc caagacggtg ttcacatggc cgaggatgta
     3241 atcgatgtcg gccttggtgg ccgccggatg ggccagatcg agattccagt cggtgtcggt
     3301 ggtaccgatg atccagtggg tgccccacgg gatgacgaac agcaccgact tctcggtgcg
     3361 caggatgatc gcgacctcgc tgacgatccg gtcgcggggc accaccacgt gcacgccctt
     3421 ggaggctcgc acccggaacc gcccgcgctg tctggacagc gcctggatct cgtcggtcca
     3481 cacaccggtg gcgttgacga ccacgtggcc gtgcacctcg gtgatcgcgc cgtcctccga
     3541 atcgcggatc gtcacccccg tcacccgatc accttcgcgc agcagcgaaa cgacctggct
     3601 cgacgagcgc accaccgcgc cgtattgagc cgccgtgcgc gccacggtca tcgtgtgccg
     3661 ggcgtcgtcg acgacggtgt cgtagtaccg gataccgcca atcagcgagc ttcgcttgag
     3721 gcctggacac aaccgcaacg cgcccgcgcg agtcaaatgc ttctgcgccg gaaccgattt
     3781 cgcgccgccg agctggtcat acagcaggat gccagccgcg acgtacggcc gctcccacca
     3841 tcgactggtc agcgggaaca gaaagggcag cggcttgacc agatgcggcg ccagcgtcgt
     3901 cagcgacaac tcccgctcgt gcagcgcctc gcggaccaac ccgaactcca actgctcgag
     3961 gtagcgcagc ccgccgtgga acatcttcga cgagcggctg gaagttccgg acgcgaagtc
     4021 gcgggcctcg acgagcgcca ccttcaaccc tcgggtggcg gcgtccaggg cggctccggc
     4081 cccgaccaca ccgccgccga tgacgacgac gtcgaactgc tcgctgccga gttgttccca
     4141 ggcctgttcg cgctgatggg ggcccaggaa ggtctgcccc ttgcccggtg cggggttcgg
     4201 gtcactcact tcgaatggac tccttgttac tcgccggtac ggttggccat tcccaggtta
     4261 ctgggtttgt cctgctctcc cccagccctt cggcgggagg tacccccacg cgtgcgcagg
     4321 gaatcagtcc aaatcgtcgt gcgccatcag ccttcgaccc gcctcggtga tggaccccgc
     4381 caacgacggg tacacggaga aggtttgggc caggtcctcg acatcgttgc cgttctgcac
     4441 cgccaacgcg atcggcatga tcagctccga ggcgatcggc gcgaccacca ccccgccgat
     4501 gaccacgccc gtcgccgggc ggcagaagat cttgacgaat cctcggcgta ggccggacat
     4561 cttggccctg gcgttggtgt tcagcggcag catcaccgtg cgggcgggca cggatccgtc
     4621 gtcgatcttt gactgcggta ccccaaccgc ggcgatctcg ggccgggtga acaccgcggc
     4681 ggcgacggtg cgcagccgaa tcggctcgag tccttcgccc agcgcgtgat acatcgcaat
     4741 gcggccctgc atcgcggcga cggatgccag cagcatcagg ccggtgcaat cacctgccgc
     4801 gtaaatgccg ggcaccgatg tgcgcgatac ccgatcgacc ttgaggtagt cgccctgtcc
     4861 gagctcgatg ccgacatgct ccaggccgag cccgctggtg ttcggcacgg acccgacggt
     4921 catcagagcg tggctgccgt cgacggtgcg gccgtcggtc atggtcacca atacgccatc
     4981 gccgtcgcgg gtcaccttct cggcgcgggc gttcttgacc agcgtcacgc cgcgcgaggc
     5041 cagcgtatcc tcgagcaccg cggcggcgtc ggagtcctcg tgcggcagga tctggtcgcg
     5101 gctcgccacg acggtgacct ttaccccgag ctcggtgtag gcgttgacga actcggcacc
     5161 cgtgacgccg gagccgacca caacgaggtg ctcgggcagc ttttcgaggt catagagctg
     5221 gcgccagttc aggattcgtt cgccatcggg ctcggcgccc ggcagcacgc gcgggctcgc
     5281 gccggtggcg atcagcacga catcggcctt gatggtgctg acatcaccat cgtgagcggt
     5341 gatcttgacg gtgtgcgctg ccatacccgg tatgtcgtcc accaactcac cgcggccggc
     5401 aatcaacgtc acgccctcgt tgcgcaggcg ctcggcgatg tcggcggact gtgcggcggc
     5461 aagcgttttg acgcgctcgt tgatctggga cagggagatt ttggcgtcct cgaactcgag
     5521 ggcgtagcca aggtcgggcg cgcgacgcag ttcggtgcgc acgccggtcg atgcgatgaa
     5581 cgtcttggac ggcacacagt cccacagcac gcaggcgccg ccgaggccgt cggaatcgac
     5641 gacggtgacg tcggcgacct cggggccgcg agccgcagcg accagcgccg cctcatagcc
     5701 ggcgggcccg ccgccgatga tcacgatgcg ggttgccacg gccttaacct aaccaaggcg
     5761 accgttgatc gcccagcagg taagcaaagc tttaggcttt ccccgtgccg ctatacgccg
     5821 cctacggatc gaatatgcat ccggagcaga tgctggagcg cgctccccat tcaccgatgg
     5881 cggggacagg gtggctgcat ggctggcggc tgacgttcgg cggcgaggac atcggttggg
     5941 acggtgcgct ggccaccgtc gtccaggatc cggcttcgaa ggtcttcgtc gtgctctacg
     6001 acatgacgaa ggaagacgag aagaacctcg accgctggga aggctcagag ctgggcatcc
     6061 acaagaagat ccgatgtcgg gtggaccgta catcctccga caccagtttc gagccggtgc
     6121 tggcatggct gtacgtggtg gacgcgtggg agggcggact gccgtcagcg cgctacctcg
     6181 gcgtgatggc cgatgcggcc gaaattgcgg gcgccccatc ggaatacgtg cacggtttgc
     6241 ggacccgccc ggcgcgcaac atcggccccg gcacatagcc ttctgtccgc gagcgaccgt
     6301 gtttgtacgc gacatgccgc ggtctgttgg tatcttgggg tcgctcacgc ggcgcgagcg
     6361 cgctgaagct gtctgtcgat acgcgcaacg aattcccacg cccggtatct gacgtcttcg
     6421 aacacgatcg gaataacagt ccaccggacg tcctgcagag ccgcctgccg tctgcgatcg
     6481 ttgcgcatcg cttcgggccc gctatgccag tccatcccgt cgtattcgac cgcgacacgc
     6541 tggtccggcc aggcgaagtc aacccgccgc agctcgccgt tcccgtcgat gatctcgtac
     6601 tgcagcactg gcgtcggcag tcctccgtcg atcatggcca gccgcgcttc gctctccatg
     6661 ggcgattcgg ccagtccgtc ggcgagcggg atcaggttgc gaacggcgac aatgccccgc
     6721 cgccccgatt gttgggcggc cgcccgccac aagtcgacgc gctgactggt cccgccgcgt
     6781 aacgcggcgt ccagtgtcgc cagggcacga ggccggcgga ggccgcgagc gacctcgacc
     6841 gccgtccaag ccggtgacgt ggcgagacgt tcgccgacct ttaccagcgg tgcgccgtcg
     6901 cggcgatgaa ccaccagccc gtctgccgag cgcagtcgag aaccgggcgg actcagcaca
     6961 tgaagatcgg cgggctcttc ggtgtcgaag ccatgcagcg ccgccgccgt tccgagacaa
     7021 acggggacct gggcgttgca cgagaagtcc aggccacgca atcgcgtgaa gtcgtcaggc
     7081 tcaccgcggc agtagatgcc cggccatatt cgttccagcg caccggaatt cacagctgac
     7141 tcgaatccgc gcctggtcag gtagttcaga acttggccgc tgctcgcgac cccgccctga
     7201 ctatcgaaca gggcctcgag ctccggctgc atgaactaga tagtcgtgct cgagtgggca
     7261 tcccggtagg cagtcacaag tcagctgtgg ataactcgcc agctgcggac gctcagtcgc
     7321 gtcgagcgac cgcggaatgc caacgcgcaa cggcgtgtcg ctgtacagac acggtcgctc
     7381 ggcgagaagg cgcttagaac tgggcggctt ggtcgatgat gttgaccaac gcgcgaacgc
     7441 ccaccgccag cgaccgctcg tccaggtcga acgtcggctg atgcaggtcg agctgcgggc
     7501 cgcggcccga ccacacgccc agccgcgcca tcgcgccggg cacctcctcg agataccacg
     7561 agaagtcctc gccaccaccg gactgacggg tgtcgcacag cacgtcggtg ccgatcgcct
     7621 cgatcgcatg cgtcaggatg cgcgtggaga tctcttcgtt gaccaccggc ggcaccccgc
     7681 ggcggtactg caggctgtgc tcgataccca gcggctgcaa caccgacgaa acgatctcgc
     7741 gcacaatcga ttccagcgaa tcccaggtgt cgcgactggc cgtccggatg gtgcccgcca
     7801 gcgtgcccgt ctgcgggatc gcattggccg ccacacccgc attcaccgca ccccacacca
     7861 tcacggtgcc gttgcgcggg tcgatgcgac gcgacagcac cccgggcaca cccgtgatca
     7921 gcgagccgag cccgtacacc aggtcgccgg tcaggtgtgg gcgcgacgtg tgcccaccgt
     7981 gcgactgcag cgtgacctcg agctgatccg cggccgacgt gatcggcccc ggccgcaccg
     8041 caaccttgcc gaccgccagc cgagggtcgc agtgcaacgc gaagatgcgc gacacacccg
     8101 tcagtgcccc ggccgagatc gcgtcgatcg cgccgccggg catcagttcc tcggcggctt
     8161 ggaaaatcag ccgcactccc accggcagtt cgggcaccga tgccagcgcc agcgcggccc
     8221 ccagcaggac tgcggtgtgc gcatcgtgac cgcaggcatg ggtgacgttg ggcaccaccg
     8281 acgcatacgg cgcaccggtg cgctcggcca tcggcagcgc atccatgtcg gctcgcagcg
     8341 cgatccgcgg cccatgctcg ggcccgaagt cgcaggtcag cccggtgccg ccgggcagca
     8401 ccttggggtt caaacccgcg tcggccaggt gcgacgcgac gaactgcgtg gttgcgaact
     8461 cctgtcggcc cagctcggga tgcgcatgga tgtgcctgcg ccacgcgatc aggtcgtcgt
     8521 agtgggcgtc cagccacagc tcggtggtgt cggcgacact catgacgccc gcctcgcctg
     8581 cagctccagc acccgctcac gctccatgtc agtttcggcg agtcggacga ccgtgcgggc
     8641 cagcatgatc gcgccctcga cgaccgcttt gtcggcactg ggctcgacag ccgcagccgt
     8701 gaacgccggt tggtggagcg acgcgccacc cgcttcgatg ccgacgatcg gatggatgcc
     8761 cggcatcacc tgcgtgacgt tgcccatgtc cgtgctgccg agcggcagcg tgccctcgac
     8821 gtcctcggcc accgggctgc ggcccagccg caccatctcg ccgcggaaga ccgaggccag
     8881 ccacgggtcc ggcgccagct ctgcatacgc gggcgcggtc tcggcaacgg tatgcgtgca
     8941 gccggtgccc accgatcccg cggcaaagca gccggccatc ttgtcttcga gctcgtgcag
     9001 cgactcggag ttcaccgcgc gcatcgtgta gcgcagctcg gcatggccgg gaatgacgtt
     9061 ggtggcctgc ccgccgtccg tgacgatgcc gtgcaccatc tgtcccggcg cgaggtgctg
     9121 ccgcagcagg ccgatcgcca cctgcgccac cgtcacggcg tcggccgcgt tcacacccag
     9181 atacggcgcg acggcggcat gcgattcgcg gccctggtaa cgaactgtca cctcggacag
     9241 cgcgagcgac cgggcggccg cgatatccaa gggtccggga tgcagcatca ccgtggccgc
     9301 gatgtcgtcg aaggttccgg ccttgagcag caacgctttt ccgccgccgg cctcctcggc
     9361 tggtgtgccg atcagcgcca cggtcaggcc cagctcgtcg gccacctctg ctaacgccag
     9421 cgccgtacct accgccgacg cggcgatgat gttgtggccg caggcatgtc cgataacggg
     9481 caacgcgtcg tattcggcgc agatgccgac gaccagcggg ccactgccgt agtcggcgcg
     9541 aaacgcggtg tccagcccgc cgggcgccgc ggtcatctca aacccatact cggcgaccag
     9601 tgcttgcgcc ttggcacagc tgcggtgttc ggcgaacgcg agttcgggct cggcgtgaat
     9661 ggagtgcgac agctcaatca gatcgccacg gcgtcggttg acggcgtctt cgacgcttat
     9721 cgacgcggtc gcggtgggca tctcagcagt atctcactgt caactggaag ccctggtgcg
     9781 agttaagctg cgcgattgtg accgaaccgg atgcggtggc cgcagaatcg gcgcgcgcca
     9841 tccgcgatcg caccggcgtc gacgaacatg acgtcgccgt agtccttggt tcgggctggg
     9901 cgcctgcggt ggcggagctg ggcgatccgg tggccgtggt gccgatggcc gagcttgcag
     9961 gcttcacccc gccgacggcc gaagggcatg gcggtcaggt gttgtcgctg cacgtcggcg
    10021 accaccgggt gctggtgttc gtcggccgga tccacgccta cgagggacac gagctgcgcg
    10081 acgtcgtgca ccccgttcgg gcggcgtgtg cggccggtgt gcgcaccgtc gtgctgacca
    10141 acgccgcggg cggcctgcgc gacgactaca ccgtcggcca gcccgtgctg atcagcgacc
    10201 atctcaacct cacggcgcgc tcgccgctgg tcggcgcgca gttcgtcgac ctgaccgacg
    10261 cgtactcgcc gcggctgcgc acgctggccc gcgaaatcga tccgtcgctg gccgaaggtg
    10321 tgtacgccgg gctgcccggg ccgcactacg agacgcccgc cgagatccgg atgctgcgca
    10381 cgctgggtgc ggatctggtg ggcatgtcga ccgtgcacga gaccatcgcg gcccgtgccg
    10441 cgggtgcgca ggtgcttgcc gtgtcgctgg tgacgaatct ggcggccggt atgacgggcc
    10501 agccgctgag ccacgacgag gtgctggagg caggccgtca gtccgcgacg cggatgggtt
    10561 ccctgcttgg ttcggtgatc gcgcgggtct gacggccgaa ccgctcactc atccagacca
    10621 cggcgcgggc catcaacggc gccgcgaaca gcatcagcag gatgcgcacc acctgcgccg
    10681 cgatgatgaa cgtgacgttg gacccggttt cgacggcggt tgcgagcacg gcgtacacac
    10741 cgccgggact ggtcgcgagg tagccctcca gtggggtgag cccggtgaag tgcgccagca
    10801 ccacaccgag gccggccgtc gcgacaccga gcgcgacgat cagggcgatc gcggcgggca
    10861 gcatgcgccc gatggcgcgc agcgattctc tcgtgaacgc caggccggcc tgccagccga
    10921 tcagcatgta gccggcctga accagcacca tcggcaccgc cagaccgaag gacaagccgg
    10981 tgacttccag cgcgaccgtc agcgccagcg gcccgagcag gcccgcgccg ggcagccgga
    11041 tcagtttgcc gacggtcgcg ccgaccagca ccagcgcgat cagcattgcg acgctcagat
    11101 accaaggggc tgaatcagtt tcgacgacag gcgcggtgcc gtgtgacttg tccgcgtgat
    11161 aaatcaaagt gaccaccacc ggcatcgatg cggttaccag accgacccgc aggtattgga
    11221 cgacggacac gacgcggtcg tcaccgccga gttcgcgggc gatcgcgacc agtccggacg
    11281 cgccgcctgc gacgagcgcc agcgatccgg tcagcggggt gacgttacgg tgcaggccga
    11341 gcagcgcgcc cgccacgatg ctgagcaaca gggtggcgac ggcgatcgcg atgacgatcg
    11401 gccagtcggc accgagcgcg ccgacagcgt cctcgtggac catcgtgccg atgtagacgc
    11461 ccagcacacc ctgtgcggcg ataccggcct ttcggggtac tcgcgcgggg gccagcgatg
    11521 ccagtgccag ggcgacgccg acgacgagcg cggcgaacag tgcagcggac ggcacgccga
    11581 cgcgggtgag cgggagggtg acgacgaccg taactgctat cagtaaggcc cacctgagca
    11641 tccgtcgcgt ccacgccatc gtaattccaa gtatgccctt gctaattcct gatagcaaca
    11701 tgcggtgatc tacgtgacat ggcgattttg ccaaggtata acttgcttat gaaccgcgtt
    11761 gactccccag ccaccgaact gcgggaatcg atgatggcgg cgacccggca gatgcgcagg
    11821 caccgccccg aacacggctt gacgctgacc cagatggaac tgctcggcga ggtcagccgc
    11881 accggcgtca ccacgccggc cgaacttgcg aaccggttgc acgtgcgggt gcagtcgctg
    11941 accgacagca tcaacgaact ggttagccgt ggcctcaccg cgcgccgtcc cgacgagacc
    12001 gatcggcgcc gtcagctcat cgaactcacc gacgcgggca ccgcgctgct ggaggccgac
    12061 cgtgccgaac gggacgcgtg gctgcacgcg accatgcgcg acaacctcac accgttggaa
    12121 ttcgacctgc tgatgctcgt cgccccggtg ctccgcaagc tggcttattc agacgcaaaa
    12181 gcgggcacac tagcgccatg acttcgtcgc ggaccggggc gcccgcgcag gagtggatct
    12241 ctcacgaccc cgatccccaa acggccgcag agctggcggc gctcagcgaa gaagagctcg
    12301 acgagcggtt cgcccaaccg ctgacgttcg ggacggcggg tttgcgtgga cccctgcgtg
    12361 gcggaccaaa cggaatgaac ctggctgtcg tcctgcgcac cacctgggcg gtggcgaagg
    12421 tgctcaagga ccgcggcctg gccggcaagc ccgtaatggt cggccgagac acccgacacg
    12481 gatccgagga cttcgctctg gccgcagcgg aagtccttgc cgcccaagga tttgcagtga
    12541 cattgatgtt cgtcgcggtg cccacacccg tgctggcgtt cgcggtgcgc catgaccgcg
    12601 ccgccgccgg catccagatc accgcgtcac acaacccgcc gtccgacaac ggctacaagg
    12661 tctacttcga gggcggatat ccgatcgtgc cgcccatcga ccacgagatc gaacgggccg
    12721 tcgcagaagc tccctatgcc gacgagattc cccggcggtc agtcaaggcg tctggcgttg
    12781 acgcgattca gcactatgtc gaccgcgcgg cgcacgttcg acacacccac gggtcggtgc
    12841 gggtggcgtt gacgccgctg cacggcgttg gcggcgaatt cgcgatggac gccctggcgg
    12901 ccgcgggaat ccccgatgtg cacgtcgtcg agagccagtt cgcccccgat ccagacttcc
    12961 caaccgtgca attgccgaat cccgaggagc ccggcgcggt cgacgccctc ctggagctgg
    13021 ccgccgatgt cgatgccgag atcgccatcg cgctcgaccc cgacgcagac cgctgtgcgg
    13081 tcgggatacc gacaccggat ggctggcgca tgctctccgg tgacgaaacc ggttggctgc
    13141 ttggtgatta catcctctcg cagatcgaac ccggtcccgt ctccgaggcc acgttggtgg
    13201 cgagctcggt ggtgtcgtcg cgcatgctgg ccgcgatcgc caacgaccac ggcgcccagc
    13261 acgtcgagac actcacgggc ttcaagtggt tgtcgcgagc cgacgtcggt cgccctggct
    13321 gcacgctggt ttatgcgtac gaagaggcga tcggacactg cgtcgatccg ggggcggtcc
    13381 gcgacaaaga tggcattagc gcgatggtgt tggcgtgcga tctggttgcc gcgctacgga
    13441 atcaggggcg cacggtgacg ggcgcgctcg acgacctcgc ccttcgccac ggtgtgcaca
    13501 ccacgaccgc ggtgtcgcga cgcgtcgcag acggcggcga ggccgaagca ctgatggcca
    13561 ggcttcgtgc gacgccgccg gacaggctgg ccggcttcga gatcacggca accgatctgc
    13621 tgcatgcgcc gacgcatctg cgcaccgacg cgctcgtctt ctccggcggc gacggcggca
    13681 actggatccg gatggttgtg cggccgtccg gaacagaacc aaagctcaag tcctacatcg
    13741 aggttcactg cgcgaacctc ggcaacatcg aggccactcg caccaaggcg cgtacagtcc
    13801 aggacgaact ggtctccgtc gcgcagcagt tctagcgcgg gccgaactgc cgatctccgg
    13861 cgtcgccgag acccggcacg atgtaggcga tgtcgttgag ccgctcgtcg atggtcgcgg
    13921 tgaacaaccg taggttcggc gctaccttct ccagtgcggc aatgccttcg ggcgcgcaga
    13981 cgacgcagac cgcggtgatg tcaacggcgt tgcgcgaatg caacagcccg atggtgtgcg
    14041 ccatcgagcc gccggtggcc agcatcggat ccagcaccat cacgggctgc gtactcaaat
    14101 cgtccggcaa cgactccaaa tacggtgtgg gctcatgggt ttcctcgtcg cgggcgacac
    14161 cgacgaaccc gacgcgggcc tccggaatga gcgcgtgcgc ctgatcgacc atgccgagcc
    14221 cggcgcgcaa caccggcacc agtagcggcg gattggccaa tcggcagcca gtggtgtcgg
    14281 ccagcggcgt tcgcaccggg actgtttcgc aggcagcgtc gcgcgtggcc tcgtacacca
    14341 acatcagggt cagatcgcgc agcgccgcgc gaaacgccgc gttgtcggtg cgctcgtc
//
LOCUS       DASRJA010000580        20071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2914472,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000580 DASRJA010000000
VERSION     DASRJA010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20071
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2914472"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..832)
                     /locus_tag="VFC01_25575"
     CDS             complement(272..832)
                     /locus_tag="VFC01_25575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83019.1"
                     /translation="MEFQHATGRKLGVGLGIGEHAQDIETVGAVDQAAHSPADGEVLG
                     VQRSYRRRHPGDVYRWTQRYPADDVLTIVAYSSRFDRAERDSPVQPPDFGDDVPPPPK
                     RREPQGACGHQRCEPDLDQGAADEDADDAQRDSGNGEQHHHKPQAGVNPPPGRADSSL
                     LHEFQPVLAPETRSVFDHVRSFQCQA"
     gene            complement(844..1254)
                     /locus_tag="VFC01_25580"
     CDS             complement(844..1254)
                     /locus_tag="VFC01_25580"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HZN83020.1"
                     /translation="MTKPTVLFLCTHNAGRSQMALGFFNRSAGDRAIAYSGGSEPADQ
                     INPAAVAVMAEKGIDITGEHPKRWTDEMIEAADVVITMGCGDTCPFIPGRRYEDWTLP
                     DPAGQPIDAVRPVRDQIEARVRELLSSLGVTVTS"
     gene            complement(1251..2006)
                     /locus_tag="VFC01_25585"
     CDS             complement(1251..2006)
                     /locus_tag="VFC01_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HZN83021.1"
                     /translation="MNRRFSATSRALLAEFVGTALLVTVVIGSGIAAAQLSPGDVGIQ
                     LLENSIATAFGLGVLILLFGPVSGAHFNPVVSLTDWLLGRRAESRISGRSVAAYVGAQ
                     VSGGIAGAVLANVMYNRAALELATKDRLTTGHLIGEVVATAGLIALIFALARTGRAAL
                     SAAAVGAYIGAAYWFTSSTSFANPAVTLGRMFSDTFAGIAPNSVLPFIAAQIVGALIG
                     LALVVALYPDAAETADQAVIPHARRSAESRSEP"
     gene            complement(2011..2496)
                     /locus_tag="VFC01_25590"
     CDS             complement(2011..2496)
                     /locus_tag="VFC01_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HZN83022.1"
                     /translation="MSRVQLALNVDNLAEGVAFYSKLFNTPPAKLKEGYANFAIAEPP
                     LKLVLIENPGHGGSINHLGVEVESSEKVHTEIGRLAGEGLFTEEEMGTTCCFATQDKV
                     WVTGPGGEKWEVYTVLADSDTFGASPESLAADPSGGCSCDACDNPQQSDDKPAAAASC
                     C"
     gene            2592..2951
                     /locus_tag="VFC01_25595"
     CDS             2592..2951
                     /locus_tag="VFC01_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017205166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2640c family ArsR-like transcriptional
                     regulator"
                     /protein_id="HZN83023.1"
                     /translation="MPKTLPMVDISAPVCCAPVAAGPLDDNAALEIALRLKALADPVR
                     VKLVSLLFGSADGEVCSCDLATAVGLTESTVSHHLTQLRRAGLVESERRGMNVYHRPQ
                     RDALIALCTVLDPNCCQ"
     gene            3177..3638
                     /locus_tag="VFC01_25600"
     CDS             3177..3638
                     /locus_tag="VFC01_25600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83024.1"
                     /translation="MNLRLIVAGTTVGGAMSLGALLVGIGVAGADPANPPIPAEQAAA
                     SDAVTPDSDSRSDEQQAEPVQDNGLPWPLNGLDLPPAPFDVPPPPPLDIPPPVQVTVP
                     VGVSLGLPAIPLGLPAPPPPQLPPPPEVPLPPAPAIPLPAPPSIPLPPPPF"
     gene            complement(3642..4724)
                     /locus_tag="VFC01_25605"
     CDS             complement(3642..4724)
                     /locus_tag="VFC01_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN83025.1"
                     /translation="MPLDHYVTLGRSGLRVSPFALGAMTFGEDPGAAGTSVEESEKIL
                     GTYLDLGGNFIDTANFYTNGHSEKILGDWFAAHPGRRERVVVASKFFGNMYPGDPNGG
                     GAGRASIVAQLDQTLRRLNTDYLDLYWLHNWDRHTPIEETMRTLDDLVRSGKIRYIGF
                     SNTPAWVTAQAQTTALLRGWTPLIALQVEYSLLARTVEGELAPLAQHQGMALVPWSPL
                     KNGFLSGKYRRGADVIDSARAAFVAGPSDAEFDVIDTVAAIADELESTSAAVALAWLL
                     ARPQTVVPIVGARRLQHLEGNLAGLEVKLSPEHLRVLDEVSAPGLNYPAEVNGATRAM
                     LQFAGTTVDGETSTVYPPLLASDVRY"
     gene            4876..5787
                     /locus_tag="VFC01_25610"
     CDS             4876..5787
                     /locus_tag="VFC01_25610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005246519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZN83026.1"
                     /translation="MPSTGGLLDEIRGRIAAHARPDLVTAIDGLLLSKVVTTATTEYS
                     LTEPLLVVMAQGGKRLLLGEQLYEYGAGDCLVVTASLPVTGHWIDVDGSGEALGMGLV
                     LRPPAIAALLVQAQPGPWSRGTPAGPAIATGKASPELLDAAARMLRLLDHPADAPVLA
                     PLITQEILWRLLAGPLGETIRQIGLADSDVSHVSRAIAWIRDSYAEPMRIDDLARLSG
                     MSASAFHRHFRAVTAMSPLQFQKRIRLQHARSLLVAHPRDIAGVGHRVGYDSPSQFNR
                     EYRRLFGAPPGQDAERLRAQAPTAASL"
     gene            complement(6361..7785)
                     /locus_tag="VFC01_25615"
     CDS             complement(6361..7785)
                     /locus_tag="VFC01_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HZN83027.1"
                     /translation="MHAYSVAATTLLSRRSVVREYLHGAYWVLPTLGIAVGVGAGAIL
                     SMIQVKDGSLIDKLMFQGTGADARGVLIVVSATMITTTGIVFSLTVLSLQIASSQFSV
                     RLLRTFLRDVPNQVVLAIFVCTFAYSTGGLLTVGERASGGEFVPKLAVTGSLALAFVS
                     IGALVYYLHHLVHSIQIDSIMEGAQSRTLALVDELFPELDTAEAAETVPPERPVTAVA
                     LFAPKSGYVQTVDVDDLADLAVETAHSMQFVVFVGDYVTAGAILGWCWRRSQAGQSTD
                     PIVLQRILRHVHIGFERTLQQDVRFGLRQMVDIALRALSPAINDPYTGVQVVHHVSAV
                     EAVLAARAIEDDVRRDDAGNVLVWLPYPGFATYLHVGCSQIRRYGIHEPLVLAALLQM
                     LSAVAQNCVDPSRRAAVKTQIDLLVRMAERELPEEADRAMVVSAADRATEVVERPGTL
                     APPPSAFGQVAAATAAQDTTTEHR"
     gene            complement(7816..8484)
                     /locus_tag="VFC01_25620"
     CDS             complement(7816..8484)
                     /locus_tag="VFC01_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HZN83028.1"
                     /translation="MSTPLPPIVSRGEWEAARADLLVREKELTRLKDTVSAARRRLPM
                     VEITEPYIFETETGSATLLDLFDGRRQLIVQHFMFGPDWEQGCDGCSLMADHIGPLSH
                     LHAKRTSFVLVSRAGLAKLLDFRERMGWELPWVSSAPSTFNEDFGATVDGEEIHSVSV
                     FLRDGDRVFHTWQAFNRGEEPFMVVFDLLDLTPYGRQETWEDSPEGWPQQPPYEWMRL
                     HDSY"
     gene            8532..8963
                     /locus_tag="VFC01_25625"
     CDS             8532..8963
                     /locus_tag="VFC01_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83029.1"
                     /translation="MRTKFEFDFVTTATPDQVIELMTDFSPNRPHRWPASSVKPFEVY
                     HLSETAADIREGQDFPKLWATWHYDWSTPGSVTLTVTDSDALQPGGYMTLTASPRDGG
                     SAVHGEWEQASKSLRGLTAVTMMRFIGPRFLASYKKVYDGL"
     gene            9123..9827
                     /locus_tag="VFC01_25630"
     CDS             9123..9827
                     /locus_tag="VFC01_25630"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN83030.1"
                     /translation="MPTENAISLGDVDVTDVSHWTAIGILLVPYGGLLAAGLEAGETL
                     LVSGATGNFGSAGVAVGLAMGASTVICPGRNEAVLADLESRFGDRIRTVRLTGDIDSD
                     RAAMQAASPDPIDVVLDLLPQNAPPTAVRAAALTVRQYGRVVLMGGVGMLGGDDFGLP
                     YPWLMLNSVTVRGQWMYPRTAIPHMIRLIRSGVLDIDHDRVTTFALDDANEAVAHAAA
                     HPGPFDRTVLLPNPPQ"
     gene            complement(9838..10722)
                     /locus_tag="VFC01_25635"
     CDS             complement(9838..10722)
                     /locus_tag="VFC01_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN83031.1"
                     /translation="MTIPVMEPSLDAATLKGWAQAIDGGPFSSLCWGERIAFDNPECL
                     TLLGALSAWTDRVRLVTTVVVPQLHDPVMLAKQLATGDMLSGGRLTVGLGVGGRQEDY
                     HAVGADVATQTRAGLAHSVAVMKHIWAGEKVTESIVPVGPPPVQAGGPQLLIGAMGPK
                     TVRSAADWADGLAGTTLDLDVTKQNELFDVARESWAQAGKPRPHLATSFWFAIGDDPQ
                     ETRAQIHRHLLRYMNWIPAEVVDAIAPTTGWAGSDDELLAVLRNFQDIGTDELHLIPT
                     SSNIDQLRRVADVATHFA"
     gene            complement(10754..11194)
                     /locus_tag="VFC01_25640"
     CDS             complement(10754..11194)
                     /locus_tag="VFC01_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZN83032.1"
                     /translation="MSVISTDTVHTGLERADRRSKAHKAGIVISALVTAFLTFDAAGK
                     LAGAPQVKEGTQALGFPAGQALVIGVVLAVCVVVFVIPRTAVLGALGITAYLGGAVTA
                     NMRVEAPLFSHTLFAVYLGVLMWIGLAMRRPELLRIVGLRGTAD"
     gene            11293..11706
                     /locus_tag="VFC01_25645"
     CDS             11293..11706
                     /locus_tag="VFC01_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN83033.1"
                     /translation="MTINTSSIAHVRLTVTDIERSRQFYESVFGWPVLIESPESADAA
                     TREALGFLFGGVIYDLGGMLVGLRPVATDRFHEDRCGLDHLAFRVASKAELDDAAAHL
                     DGLGVEHEPIKDIGPSFILEFRDPDNIALELTAPK"
     gene            complement(11707..12840)
                     /locus_tag="VFC01_25650"
     CDS             complement(11707..12840)
                     /locus_tag="VFC01_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HZN83034.1"
                     /translation="MTTTDRHRLLSYLGPNWFASVMGTGIVATAGATLPLHVPGLRGF
                     AEAVWVAATVWLVILIIANVAHWMRNPTVARSHAYNPQMGHFYGAAPMALLTVGAGAV
                     LVGKDLIGEQAAVHLDWVLWTAGTLGGLFTAISIPYLMFTQYRVEPDAAFGGWLMPVV
                     PPMVSAATGALLIPQIASGTGRQTMLYGCYAMFGLSLVASLIIITMVWSRLAHYGTSG
                     TARVPTLWIVLGPLGQSITAAGLLGTNAALAVDSDLADGMAVFAILYGVPVWGFAVLW
                     IALATSLTIRTLRRGMPFALTWWSLTFPVGTFVTGTTQLAVHTGLPSFRVAAAIAYVG
                     LLSTWCLVAVRTFRGGVRGNIFAPPSADPVKAHKDPPATTSSA"
     gene            12932..14239
                     /locus_tag="VFC01_25655"
     CDS             12932..14239
                     /locus_tag="VFC01_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZN83035.1"
                     /translation="MVAVTDTSPEATPAPAAVDGAAATPRNIGLVPTASLLTGAAIAT
                     VWFWIPLTILIIGVSSIPSGIGFALSAVVFIYLMRGVEHVERLRSEAVFGLGIGVPPR
                     RISPYAGFQRWLHQLWLDISGARFWKATAHHYLRMAYDMLAAGMALTLLAWAFLGPAA
                     AIAIRQSDEDAGLSFMSPPLAWLSALVAVVGAAAILVFAPAVDAVIDRWLLSPSPTAA
                     LQYQVSALADARQGAVSSAQTERHRIERDLHDSVQPRLVSLAMTIGLAQTKLDSDLPA
                     AKQLIAEAHDDAKSALVELRNVVRGIAPTILSDRGLDAALSAVVQRSAVPTTLNVHLP
                     RRLPDEAESVAYFVVAEALTNVAKHANATQAVVTVRLDESANQLYVTVFDDGRGGASI
                     TTDENATGLRGLEERVRAAHGTFTVSSPATGPTIVTAVLPCAS"
     gene            14227..14871
                     /locus_tag="VFC01_25660"
     CDS             14227..14871
                     /locus_tag="VFC01_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN83036.1"
                     /translation="MRIVIAEDSALLRAGIERILTDAGHEVVAGVPDATNLLRLVNEK
                     RPDLAIVDVRMPPTFTDEGIRAAALLRSQNPESPVLVLSHYVEERYAADLIASDTKGF
                     GYLLKDRVADVPAFLDAVEVVGGGGTVLDPEVVSQILVRSHRRSALDELTPREREVLQ
                     LMAEGKTNSAIASALYISVGSAEKHIASIFTKLDLAPDESENRRVLAVLRFLES"
     gene            14893..15774
                     /locus_tag="VFC01_25665"
     CDS             14893..15774
                     /locus_tag="VFC01_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZN83037.1"
                     /translation="MTTIAPPPPAPATPPPPLSPGGRTAFRIALIAAAVVLVVGTLAS
                     LGAVAWGVASFRVITDSKPLPSGMRSVVVDTGSVPIAIRITTDREAREPRADLRMVSS
                     MRAGANPLVVNADPAGARVTINSGGSTFLDLGRAGEITLVLPPDLARKLKVTAQQDTG
                     VVLAQADIDQLIAHTDTGAVVLSGAARRIEIHTDNGDVVTRDPIAVSESFSATTDNGD
                     IKVDFSEAAPRTVDVTTDNGDVVIALPQRGPYLVNVNSTDGDTEIRVPQTTDRQNAAA
                     VVTARSDTGDVIIDDLR"
     gene            complement(15755..16717)
                     /locus_tag="VFC01_25670"
     CDS             complement(15755..16717)
                     /locus_tag="VFC01_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN83038.1"
                     /translation="MSDFVRDTEPYRRELLAHCYRMLGSAHDAEDVVQETYLRAWRGY
                     QQFENRSSVRTWLYRIATNTCLTALGHGSRRMLPSGLGGPADDPHVDSESAPNVVWLQ
                     VIPDSWVDDPAVVVTSRESLRLALIASLQHLPAQQRAVLLLRDVLAFPAAEVAGMLEI
                     SVAAVKSALQRARARMQDIAPSVDDVIEPTEPRARELLDQYMTAFENADTALLEQALR
                     HDAALEMVGSRTWFAGRATCLPYLAGVVGAPGDWRMVTIAANGQPAVAAYRRDEDGVL
                     RAFGVAVLDVTAAGIARIVVFGDAGLVTLFGLPLIPESATADRR"
     gene            complement(16714..17115)
                     /locus_tag="VFC01_25675"
     CDS             complement(16714..17115)
                     /locus_tag="VFC01_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZN83039.1"
                     /translation="MSITFSDTARQVLDGPHLAVVATSNADGRPQSSVIFVKRDGDTV
                     VFSTIKGRLKTRNMARDPRISLLVIDKEPGRYIEIRGTVEITDDPEKRLLYEMYDRYM
                     GGMAPPPEPDAERLIVRITPEKVYQFPPAAA"
     gene            complement(17173..18666)
                     /locus_tag="VFC01_25680"
     CDS             complement(17173..18666)
                     /locus_tag="VFC01_25680"
                     /inference="COORDINATES: protein motif:HMM:NF023284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3376 domain-containing protein"
                     /protein_id="HZN83040.1"
                     /translation="MLAAIREPDERLDADSLRKIFTQMSYSRSQAHYVIYEFLRDPEG
                     SDANEIIEAAKHVFEPYRESRAEAFERQQMLVAGDRTSPFQPPHPSTLEPDDVPGMPR
                     LPFAAPEGNTWDWGWPVADRVLRWWGRAMARLEALPDQERRGQIKTAMQIVANCQREV
                     AGLCDVLDVVARGGPTDDKSPAAQRARLRRLYETGSDELTAIYGPGAGSLSTRLGICM
                     TTAVGAIQTEFQQESLSNLVKLSLGVEVLSGMLAWGSDDYDVPEFRFHNVTPGVDVPP
                     GIPLEWLGNQPSWPLKKLYGQRLGHFGAFASEEGRNNDWLWGRLDGASELSRQLLAAA
                     GVNEDEADRLRKALIEEILKNEGQSAAEVIDNARKAFKLPARELIDGMASRDDGRSLM
                     KLEDTLWTLSHQFGTAGFWLRVVLAPDWSRDEMQGRNFAQKLWIPGLRAACGIIRRRI
                     RKGLPKEMSMKSRHALRAQEAAPPPTIVERPSEPSIGARAGQASDED"
     gene            complement(19738..>20071)
                     /locus_tag="VFC01_25685"
     CDS             complement(19738..>20071)
                     /locus_tag="VFC01_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83041.1"
                     /translation="DVQSAALRVAQNELDLRLLYEAVIDVDLARLKLWTRQLPIDVAA
                     RDAALVLADVAALERVWERTRHGVDPAAPVDAAMHDLRKAADVEDLAAVDRAAAALNR
                     AVGGLHDL"
BASE COUNT         3509 a         6732 c         6358 g         3472 t
ORIGIN      
        1 tgagtgaatc gcagtggacg cgtatggcaa ctcctgactc tagcgtacat tccgagttac
       61 ggcaggtaga tgtatgtttg tgcagctatc agctgtgctg ggcaaccacc gtgtcggcta
      121 attcgaatcc tgccgggggc acccacgttt gccctggtaa gacaggatca agcggatcgg
      181 aatgtttacc ccaccgctgc cctttcgggt tctttcattc gctggcgacc gccgcgtttc
      241 gcgcccttgg ctgacctggc cgctgctacc ttcacgcctg gcactggaag gagcggacgt
      301 ggtcgaagac ggaacgggtc tccggggcca gcactggctg gaactcatgg aggaggctgg
      361 agtcagcccg gcccggcggc gggttcacgc ccgcctgtgg cttgtgatga tgctgctcac
      421 cgttgccgct atcgcgctga gcgtcgtcag catcttcgtc cgcggcgccc tggtcgagat
      481 cgggctcgca tcgttggtgg ccgcaggcgc cctgtggttc acgacgtttt ggtggcggcg
      541 gcacgtcatc gccaaaatcc ggtggctgga cgggactgtc acgttccgct cggtcgaacc
      601 tggaggagta ggcgacgatg gtcaatacgt cgtctgccgg gtaacgctgc gtccaccggt
      661 agacatcacc cgggtggcga cgacggtagg accgctggac gcccagcacc tcgccgtcgg
      721 cgggactatg cgctgcctga tcgaccgcac caacagtttc aatgtcctgc gcgtgttccc
      781 ctatgcccag cccgacgcca agcttccgtc cggtcgcatg ctgaaattcc aatggcttag
      841 gcgctacgaa gtcaccgtga cgccaagcga actcaacagc tcgcggacac gcgcctcgat
      901 ctggtcgcgc acaggccgca cagcgtcgat cggctgccca gccgggtccg gcaacgtcca
      961 atcctcgtag cgccgaccgg ggatgaacgg gcacgtgtcg ccgcagccca tcgtgatcac
     1021 cacgtcggcg gcctcaatca tttcgtcggt ccagcgcttc ggatgttcgc cggtgatgtc
     1081 gatgcccttc tcggccatca ccgcgacggc agccggattg atctggtcgg ccggttccga
     1141 accacccgag tacgcgatcg cacgatcgcc cgccgatcgg ttaaaaaatc cgagcgccat
     1201 ttgcgagcgt ccggcgttgt gagtgcacaa gaacagcacg gtgggtttgg tcatggttca
     1261 ctacgtgatt cagcgctgcg gcgcgcgtgc ggaatgacag cctgatcggc ggtctctgcg
     1321 gcgtcgggat acagcgcgac gacgagcgcc aggccgatca acgcgccgac gatctgcgca
     1381 gcaatgaacg gcagcaccga gttgggcgcg atacccgcga aggtgtcgga gaacattcgg
     1441 ccaagtgtca cagcgggatt cgcaaacgac gtggagctgg tgaaccagta cgccgcgccg
     1501 atgtacgcac cgacagcagc agccgacaag gctgctcggc cagttcgggc cagcgcgaaa
     1561 atcaacgcga tcagtcccgc ggttgcgacc acttcaccga tgagatgacc cgtggtcaac
     1621 ctgtccttgg ttgcgagttc cagcgcggcc cggttgtaca tgacgttggc gagaaccgcg
     1681 cccgcgattc cgccggacac ctgcgcgccg acataggcgg ccaccgatct gccactgatg
     1741 cgcgattccg cacgccgacc caacagccag tcagtcagcg acacaacagg attgaaatgc
     1801 gcccccgaca ccgggccgaa gagcaggatc agcacgccaa gaccgaacgc tgtcgcgatc
     1861 gagttttcca gcagctgaat accgacatcc cccggcgaca gttgcgcggc cgcgatgcct
     1921 gacccgatga cgaccgtcac cagcagcgcg gtccccacga actcggcgag aagcgctcgc
     1981 gaggttgcgc tgaacctgcg gttcacgccg tcagcagcac gacgccgctg cggcaggctt
     2041 gtcgtctgac tgttgggggt tgtcacacgc atcgcacgaa catccgccgc tcgggtcggc
     2101 agcgagcgac tcggggctgg cgccgaacgt gtcggagtcg gccagcacgg tgtacacctc
     2161 ccacttttcg ccgccaggcc cggtcaccca caccttgtcc tgggtggcga agcaacaagt
     2221 cgtgcccatc tcctcctcgg tgaacaggcc ttcgccggcc agccggccga tttcggtgtg
     2281 caccttctcg ctggattcga cttcgacgcc gagatggttg atggaaccgc catggccggg
     2341 gttctcgatc agaaccagct tgagcggcgg ctcggcgatg gcgaagttgg cgtagccctc
     2401 tttcagcttc gccggtgggg tgttgaacaa cttcgagtag aacgcgaccc cttcggccag
     2461 gttgtcgacg ttgagagcga gctgaacacg agacatgtcg gcctcctacc tgtgtgacat
     2521 atatcgaact ctggtgcggt caataatgcc acctctttga tatatgtcaa tctttctggc
     2581 aggatcgatg tatgccgaag acgttgccga tggtcgacat ctctgcgcct gtctgctgtg
     2641 caccggttgc ggccggcccg cttgatgaca acgccgcgct cgagattgcg ttgcggctca
     2701 aggcattagc cgatccggtg cgcgtcaagt tggtgtcgct tctgttcggc tcagccgacg
     2761 gcgaagtgtg cagttgtgac ctcgcgacgg cggtaggcct gaccgaatcg acggttagcc
     2821 atcacctgac tcaactgcgc cgggcgggct tggtcgagtc cgagcggcgg gggatgaacg
     2881 tctatcaccg cccgcagcgc gacgcgctta tcgccctgtg cacggtcctc gacccgaact
     2941 gctgccagta ggcgcgccgg acactcgaga cttttcggta agcccggtcg gctttgccgt
     3001 cccccctccg gtcgccgcca cggcaaggtc tgcgacatgc cgcggagtgg gccgagattg
     3061 ctcacggcgc gcgtggcgat aattgacgcc gcctacgcga ctcgaaatcg ccctgctcgc
     3121 agcgacattc ggctcggcta cgcctattgt gagcgcagtc gactctagga gtgatcttga
     3181 atttgcggct catcgtggcc ggaacaaccg tcggaggcgc gatgagccta ggcgcgctgc
     3241 tcgtcggcat cggtgtggca ggcgccgatc ccgccaaccc gccgatcccc gccgagcagg
     3301 cggcggcttc cgacgctgtc acccccgata gcgacagccg gagcgacgag cagcaggctg
     3361 agcccgttca ggacaacgga ttgccttggc cattgaacgg gctcgatcta ccaccggctc
     3421 cgttcgacgt cccgcctccc ccaccgctcg acattccgcc gcccgtacag gtgacggttc
     3481 cggtcggggt gtcactgggc cttccggcga ttccgttggg gctgcccgcg ccacctccac
     3541 cgcagctgcc tccaccgccc gaggtgccgt tgccgcccgc tccggcaatt ccgctgccgg
     3601 caccgccgtc gatcccgttg ccgcctccgc cgttctgaca atcagtaccg gacgtcgctg
     3661 gcgagcagcg gcggatacac ggtcgaggtt tcaccatcca ccgtggtgcc cgcgaattgc
     3721 agcatcgcgc gcgtcgcgcc gttcacctcc gccgggtaat tgagcccggg cgccgacacc
     3781 tcgtcgagga cgcggagatg ctctgggctc agcttcactt ccagcccggc caggttgcct
     3841 tcgaggtgtt gcagccgtcg cgccccgacg atcggtacga ccgtttgtgg ccgcgcgagc
     3901 agccacgcca aagctactgc tgcagaggtg ctttcgagct catctgcgat tgcggcgacg
     3961 gtatcgatga catcgaactc ggcgtcactt ggaccggcga cgaaggccgc acgcgccgag
     4021 tcgatgacgt cggcgccccg ccggtacttc cccgacagga aaccgttctt cagcgggctc
     4081 cagggcacca gcgccatgcc ctgatgttgt gcaagtggcg cgagttcgcc ctcaacggtt
     4141 cgggccagca gcgagtattc gacctgcagc gcgatcagcg gggtccagcc tcgcaacaac
     4201 gcggtggtct gcgcttgcgc tgtcacccat gccggcgtgt tggagaaccc gatgtagcga
     4261 atcttgcccg agcgcaccaa gtcatcgagc gtgcgcatgg tctcctcgat cggggtgtgg
     4321 cggtcccagt tgtgtagcca gtacagatcg aggtagtcgg tgttcaggcg gcgcagggtc
     4381 tggtccagct gagcgacgat cgacgcgcgg cccgccccgc cgccgttcgg gtcaccggga
     4441 tacatgttgc cgaagaactt cgacgccacg acgacgcgct ctcgtcggcc tggatgggcg
     4501 gcgaaccagt caccgagaat cttctcggag tggccattgg tgtagaagtt ggcggtgtcg
     4561 atgaagttgc cgccgaggtc gagataggtc ccgaggatct tctcggactc ctcgacgctc
     4621 gtgccggcgg cgccggggtc ttcgccgaag gtcatggcgc cgagcgcgaa cgggctgacc
     4681 cgcagcccag accggccgag cgtgacgtag tgatcgagtg gcacgattgc gctccttatc
     4741 gctgaaatga acagtctcat cacaccgcta tgggcccggc caccggtaga ttaatcgacg
     4801 cgcgttcttg cacaatcctg tcgaacttgt cctgatcgtg ttgcacgctc atcgccggcc
     4861 atgttttcgt gagctgtgcc cagtaccggc ggtcttctcg acgagatccg tggacgcatc
     4921 gcagcccacg ccaggccaga cctggtgacc gccatcgacg ggctgctcct gtcgaaagtg
     4981 gttacgaccg cgactacgga gtactccttg accgagccgc tactggttgt catggcccag
     5041 ggcggcaagc gtctcttgct cggcgagcag ctatacgagt acggggcggg cgattgcctc
     5101 gtcgtcaccg cctccctccc cgtcactggc cactggatcg acgtggacgg aagcggagag
     5161 gctctgggca tgggactcgt actgcgccca cccgcgatcg ccgcgttgct cgtgcaggcc
     5221 cagccgggcc cgtggtcgcg cggaacacca gccgggcccg cgatcgcaac cggcaaggcc
     5281 agccccgaac tactcgacgc cgccgcacgg atgctgcggc tactcgacca tcccgccgac
     5341 gcgccagtgc tcgctccgct gatcacccaa gaaatcttgt ggcggctgct cgccggcccg
     5401 ctgggagaga caatccgcca gatcggcctg gccgacagcg acgtgtcaca cgtcagccgc
     5461 gcaatcgcct ggatccgcga cagctacgcc gagccgatgc gcatcgacga cctcgctcgg
     5521 ctgtcaggga tgagcgcctc ggcgttccat cgccacttcc gagcggtaac agccatgagc
     5581 ccgttgcagt ttcagaaacg catccgcctg caacacgccc gctcgctact ggtcgcccat
     5641 ccccgggata tcgcaggcgt cgggcaccgc gtcggctatg acagcccgtc acagttcaat
     5701 cgcgaatacc ggcggctgtt cggcgcaccg cccggtcagg acgcggaacg ccttcgcgca
     5761 caggcgccta cggccgcgtc gctatagaca aaactcctga ggtggatgaa acatatggtg
     5821 cgaatgtgga accaggcccc cctccccccg gcggggcccg gccacggatc aattgtggct
     5881 cagtgcatgc ccaaaggaat cgggtgtttc cctagcttga gtggcctgag ggtggtcacc
     5941 tccaccactt ccacggttgg tcccaatgac cgggtggcgg cacccccggt agttgaccaa
     6001 tatgtccagg cgtcgggaca aagtgcccag gcggcggcac tgcatcacac cagccccacc
     6061 agcaccagtc atcagcttgt gcctggcctg cgccaatgcc gatcgatgcg agtccgagcg
     6121 cccctgccac agccgctccc gctgcgatcc ccttcagtct catcgtgaac tcctctatca
     6181 cctggctgcc ctgccgctac caaggaaggc gccttgcccg cacagtggcg aaaacggcaa
     6241 ggcgctcacg actatttctg atgtgtctat cgccccagtc gacctgcgcg ttagccacca
     6301 gcaaggggca gcgaccgtga tcgcttgtcc cgtttcgttt tttggatagc gatgcccgca
     6361 tcagcggtgt tcggttgtcg tgtcctgcgc ggcggtcgcg gcagccacct gaccaaatgc
     6421 agagggcggc ggtgccagtg tgccgggccg ctcaaccact tcggtcgcac ggtcagcagc
     6481 gctgacgacc atggcgcgat cagcctcctc cgggagctcg cgctcggcca tccgaacgag
     6541 gagatcaatc tgcgtcttca ccgcagcgcg acgcgacgga tccacgcaat tttgggccac
     6601 cgcgctgagc atctgcagca atgctgcgag gactaacggc tcgtgaattc cgtacctgcg
     6661 aatctgcgaa caaccgacat gcaggtaggt ggcgaaacct ggatagggca gccataccag
     6721 cacgttgccc gcgtcgtcac gccgtacatc gtcctcgatt gcccgcgcgg ccaggaccgc
     6781 ctcgaccgcc gaaacatgat gcacgacttg gactcccgtg tacgggtcat tgattgccgg
     6841 cgacagcgcg cgcagcgcga tgtccaccat ctgtcgcaga ccgaacctga cgtcctgctg
     6901 cagtgtgcgt tcgaatccga tatggacgtg ccgcagaata cgttgcaaga caatcggatc
     6961 ggtggactgg cccgcctggc tcctgcgcca gcaccagcca aggatcgcgc ccgcagtgac
     7021 gtaatcaccg acgaatacca cgaactgcat cgagtgcgcg gtctcgacgg ccagatccgc
     7081 gaggtcgtca acgtccaccg tctgtacgta gcccgatttc ggcgcgaaca gcgcgactgc
     7141 tgtcaccggt cgttccggcg ggaccgtctc ggccgcttcg gcagtatcga gctctggaaa
     7201 gagttcgtcc accagcgcga gcgtgcgact ctgagcaccc tccatgatcg agtcgatctg
     7261 aatcgagtgc accaggtggt gcaggtaata gaccaaggcg ccaatgctga cgaacgcgag
     7321 cgccagcgac ccggtgaccg caagcttggg cacgaattcg ccgccactcg cgcgctcccc
     7381 caccgtgagg agcccaccgg tgctgtacgc gaatgtgcac acgaagatcg ccagcaccac
     7441 ttggttgggc acgtcacgca ggaacgtgcg cagcaagcgc accgagaact ggctcgaggc
     7501 gatctgcagg gacagcaccg tcagcgagaa gacgatgccg gtggtggtga tcatcgtcgc
     7561 ggacacgacg atgagcaccc ctctggcgtc ggccccggtg ccctggaaca tgagcttgtc
     7621 gatgagcgag ccatctttca cctggatcat cgacaggatc gccccagcgc caacgccgac
     7681 cgcgatgccg agcgtcggca gcacccaata ggcgccgtgc aagtactccc tcaccacgct
     7741 gcggcggctc agcaaagtgg ttgcggccac ggaataagca tgcacctgcg gcgagcgccg
     7801 gatgcagagt ttgcgtcagt aggagtcgtg cagtcgcatc cactcgtacg gcggttgctg
     7861 cggccagccc tccggagagt cctcccacgt ttcctggcgt ccatatgggg tgaggtcgag
     7921 caggtcgaac accaccatga acggctcctc cccacgattg aacgcctgcc aggtgtggaa
     7981 cacgcgatca ccgtcgcgca ggaacacgct gaccgagtga atctcttcac cgtcgacggt
     8041 tgcaccgaag tcctcgttga acgtcgacgg cgctgacgac acccacggca gttcccagcc
     8101 catgcgctcg cggaaatcca acagcttggc cagcccggca cgggaaacca gtacaaacga
     8161 cgtgcgtttc gcgtgcaggt gcgacagcgg cccgatgtgg tcggccatca gcgagcaccc
     8221 gtcacagccc tgctcccagt cgggaccgaa catgaagtgc tgcacgatca gctgcctgcg
     8281 tccatcgaac agatccagca gtgtcgcgga gcccgtctcg gtctcgaaga tgtagggctc
     8341 ggtgatctcg accatgggca ggcgccggcg cgcggcgctc accgtgtcct tcagccgggt
     8401 gagctccttc tcgcgcacca gtaggtccgc acgcgccgcc tcccactcgc cacgcgacac
     8461 gatcggcggc aatggtgtac tcatgccgcg agcatgccac gcagagccga caccgctgat
     8521 acgttctgcc cgtgcgcacc aagttcgaat tcgacttcgt gacgaccgcg acacccgatc
     8581 aggtcatcga gctgatgacc gacttctccc ccaaccgccc gcaccgctgg ccggcgtcct
     8641 cggtgaagcc gttcgaggtg tatcacctca gtgagaccgc ggccgatatc cgcgagggcc
     8701 aagacttccc gaagctatgg gccacttggc attacgactg gtcgactcct ggttcggtga
     8761 cactgacagt gacggacagc gacgcgctcc agcctggcgg ctacatgaca ctgacggcgt
     8821 cgccacgaga cggcggaagt gccgtacacg gcgaatggga acaggcctcg aaaagcctca
     8881 gagggctcac cgcggtgacg atgatgcggt tcatcggccc gcggttcctc gcgtcgtaca
     8941 aaaaggtcta cgacgggtta tgactccgaa ccggagatct ggtctggtgc gactccaccg
     9001 tgagatcccg cgacgacgca ctcgtaccgg atatcacatt gcagggctgg acttttcgaa
     9061 gcgaaggcgg cagacgtctg tcgcactact tccacgatgg tccgttcgca gagcagatgg
     9121 tcgtgcccac cgagaatgcc atctcgctgg gcgatgtgga cgtcacggat gtgtcgcact
     9181 ggacggccat cgggattctg ctggtgccct acgggggcct gctggctgcg ggtctggaag
     9241 ccggcgagac gctgcttgtg agcggcgcga cggggaactt cggtagcgca ggcgtcgcgg
     9301 tcggtttggc gatgggcgca tcgacggtga tctgccccgg tcgcaatgag gccgtgctcg
     9361 cggatctgga gtcgcgattc ggcgaccgga tccgaacggt caggctcacc ggcgacatcg
     9421 actccgaccg cgccgccatg caagccgcgt cacccgaccc catcgacgtg gtgctggatc
     9481 tactccccca aaacgctcca ccgacagcgg tccgcgccgc ggcgttgacc gtgcgtcagt
     9541 acgggcgggt ggtgctgatg ggtggggtcg gcatgctggg cggcgatgac ttcgggctgc
     9601 cctacccgtg gctgatgctc aactcggtca ccgtccgcgg ccagtggatg taccccagaa
     9661 cggccatccc gcacatgatt cggctcatcc gcagcggcgt gctcgatatc gaccatgacc
     9721 gggtcaccac cttcgcactc gacgatgcca atgaggccgt tgcacatgcc gcggcacacc
     9781 cagggccctt cgatcgaact gtcctgctgc cgaatccgcc gcagtgacgg ccagcagcta
     9841 ggcgaaatgc gttgcgacgt cggcgacgcg gcgcaactgg tcgatgttcg agctcgtggg
     9901 gatgaggtgc aattcgtcgg tgccgatgtc ttggaagttg cgcagcaccg cgagaagctc
     9961 atcgtcactg cccgcccatc cggtggtggg tgctatcgcg tccaccacct cggccggaat
    10021 ccagttcatg tagcgcagta ggtgccggtg tatctgcgcc ctggtctcct gcgggtcgtc
    10081 tccgatggcg aaccagaacg aggtcgccag gtgcggtctg ggcttaccgg cctgcgccca
    10141 tgactcgcgc gcgacgtcga acagctcgtt ctgcttggtg acgtcgagat cgagcgtggt
    10201 gcccgccagg ccgtcagccc agtccgccgc gctgcggacg gttttcgggc ccatcgcgcc
    10261 gatcagcagc tgcggcccac cggcctgcac cgggggcggc ccgaccggga cgatggactc
    10321 ggtgaccttc tcgccggccc agatgtgctt catgaccgcg acgctgtggg ccagcccggc
    10381 ccttgtctgc gtggcgacgt cggcgccgac ggcgtggtag tcctcctggc gaccgccgac
    10441 gccgaggccg acggtcagcc ggccgccgct gagcatatcg ccggtggcca gctgcttcgc
    10501 caacatgacg gggtcgtgca gttgaggcac caccaccgtc gtcaccaacc ggacgcgatc
    10561 ggtccatgcc gaaagcgcgc cgagcagcgt caggcactcg ggattgtcga acgcgatgcg
    10621 ctcgccccag cacagcgacg aaaacgggcc accgtcgatg gcctgggccc agcccttcag
    10681 cgttgccgcg tccaggctgg gctccatcac cggtatggtc atcccgattc gcacgacggc
    10741 gattgtggca tggtcagtcg gcggtccctc gaagcccgac gatgcgcagc agttccgggc
    10801 gccgcatcgc gaggccgatc cacatcagca cgccgaggta cacggcgaac agcgtgtgcg
    10861 aaaacagcgg ggcctcgacg cgcatgttgg ccgtgaccgc accgcccaga tacgcggtga
    10921 tgccgagcgc gccgagcacc gcggtacgcg gaatcacaaa gacgacgaca caaaccgcca
    10981 gcacgactcc gatgaccagc gcctggccgg ccgggaatcc caaggcctgg gtgccctcct
    11041 tgacctgcgg cgcaccggca agcttgccgg ccgcatcgaa agtcagaaac gcggtaacga
    11101 gggcactgat gacgattccg gccttgtggg cctttgatcg acgatcggcg cgctcaagac
    11161 ccgtgtggac ggtatcggtg gaaatgacgc tcatgatgat gctccccttg ctgatgtcga
    11221 gacagtgacg cctagacaga ccggggcggt gccggaaaat catcgcgcgg ccgcgactgg
    11281 cagcatgggc caatgacgat caacaccagc tccatcgcgc atgtccggct caccgtgacc
    11341 gacatcgagc gatcgcgaca gttctacgag agcgtgttcg gctggccggt gttgatcgag
    11401 tcaccggaga gcgccgatgc ggcgacccgt gaggcgctcg gctttctgtt cggcggcgtg
    11461 atctacgacc tgggcgggat gctggtcggc cttcggcccg tcgcgaccga tcggttccac
    11521 gaggaccggt gcggacttga ccacctggcc ttccgggtgg ctagcaaagc cgaattagat
    11581 gatgccgcag cacatctcga tgggctcggg gtcgaacacg agccgatcaa ggacatcggg
    11641 ccgtcattca ttctggagtt ccgcgacccg gacaacatcg cgctggagct caccgccccg
    11701 aagtagtcag gcggacgacg tagtggcggg cggatccttg tgcgccttga cgggatctgc
    11761 cgatggcggc gcgaaaatgt tgccccgcac gccgccccgg aatgtcctga ccgccaccag
    11821 acaccaggtg ctcagcaggc cgacgtaggc aatcgcggcc gcgacgcgga acgacggcag
    11881 acccgtgtgg acggccagct gcgtggtgcc cgtgacgaag gtgcccaccg ggaaggtcag
    11941 gctccaccac gtcagagcga acggcatgcc gcgccggagt gtgcggatcg tcagcgatgt
    12001 ggccaacgcg atccacagca ccgcgaatcc ccataccgga acgccataca agatcgcaaa
    12061 gacggccatg ccatctgcga ggtccgagtc gacggccaga gcggcgttcg taccgagaag
    12121 ccctgcggcc gtgatggatt ggccaagcgg acccaacacg atccacagcg tgggcacgcg
    12181 ggcggtgcct gaggtgccgt agtgcgcgag gcggctccac accatcgtga tgatgatcag
    12241 ggacgcgacg agcgacagcc cgaacatcgc gtagcagccg tagagcatgg tttgcctgcc
    12301 ggttcccgac gcgatttggg ggatcagcag cgcacccgtc gccgccgaca ccatcggtgg
    12361 caccaccggc atcagccagc cgccgaatgc ggcgtcaggt tcgacgcggt actgggtgaa
    12421 catcagatac gggatgctga tcgcggtgaa cagtccgccg agcgtgcctg ccgtccacag
    12481 cacccagtcg aggtggacgg cggcctgctc gccgatcagg tccttgccga cgaggaccgc
    12541 tccggcaccg acggtgagca acgccatcgg cgccgcgccg tagaaatgcc ccatctgcgg
    12601 gttgtaggcg tgactccggg ccacagtcgg gttgcgcatc caatgcgcga cgttcgcgat
    12661 gatcaggatc accagccaga ccgtcgccgc cacccaaact gcctcggcga acccccgcaa
    12721 acccgggaca tgcagcggca gcgtcgcgcc cgccgtggcc acgatcccgg tacccatgac
    12781 cgacgcgaac cagttcgggc ccaggtagct cagcaatcgg tgtcggtccg tcgtcgtcat
    12841 gggtcgacgg tactgatgtg ggggttttcc cccgggtgct tctcgggaaa acgctgtgtc
    12901 gctcctgccc tgcggtccat aggttgaagg catggtcgca gtcaccgaca cgagtcccga
    12961 agccaccccg gcacccgctg ccgtcgacgg cgcagccgca acaccccgca acatcgggct
    13021 ggtgccgacc gcctcgttgc tcaccggcgc ggcgatcgca acggtctggt tctggattcc
    13081 cttgacaatc ttgatcattg gcgtttcgtc gattcccagc ggtatcggct tcgccttgtc
    13141 cgccgtggtt ttcatctacc tgatgcgcgg cgtcgagcac gttgaacgtc tgcgcagcga
    13201 ggcggtgttc ggcctcggca tcggcgttcc gccacggcga atatcgccct acgccggctt
    13261 ccagcgctgg ctgcatcagc tgtggctgga catcagcggg gcccggttct ggaaagcgac
    13321 cgcccaccac tacctgcgga tggcctacga catgctggct gcgggtatgg cgctcacgct
    13381 gctggcctgg gcattcctgg ggccggccgc cgcgatcgcg atccggcaga gcgacgagga
    13441 cgcggggctg tcgttcatgt cgccgccgct ggcctggctg tcggccctcg tcgcggtggt
    13501 cggcgcggcg gcgatcctgg tattcgcgcc tgccgttgac gccgtcattg accgctggct
    13561 gctgtcaccg tcgccgacgg ccgcgctgca ataccaggtc agcgcgctgg ctgatgcccg
    13621 gcagggcgcg gtgtcatcgg cgcagaccga acgccaccgc atcgaacgcg acctgcacga
    13681 cagcgtgcag ccgcggctgg tctcgctggc catgaccatc ggcttggcgc agaccaagct
    13741 cgactccgac ctgcccgcgg ccaagcagct gatcgccgag gcacacgacg acgccaagag
    13801 cgcgctcgtc gagctgcgca acgtggtgcg cggcatcgcg ccgacgattc tgtccgaccg
    13861 cgggctggat gccgcgttgt ctgcggtggt gcagcggtcg gcggtcccga cgacactgaa
    13921 tgtgcatctg ccgcgccggc tgccggacga ggcggagtcg gtcgcgtact tcgtcgtcgc
    13981 agaggcgctg accaacgtcg cgaaacacgc gaacgccacg caggcggtgg tcaccgtgcg
    14041 gctcgacgaa tcagccaacc agctgtacgt cacggtgttc gacgacggac gcggcggcgc
    14101 gtcgataaca actgacgaga acgccaccgg cctgcgcggt ctggaggaac gcgtgcgtgc
    14161 cgcgcacggc acgttcaccg tgtccagccc cgcgacgggg cccaccatcg tcaccgcggt
    14221 gctgccatgc gcatcgtgat cgcggaggac tcggcgctgc tgcgggcggg catcgagcgg
    14281 atcctcaccg acgcaggcca cgaggtggtc gcgggtgtgc ccgacgcgac gaacctgctg
    14341 aggctggtca acgagaagcg gcccgatctt gcgatcgtcg acgtgcggat gccgccgacg
    14401 ttcaccgacg aaggcatccg ggccgcggcc ctgctgcgca gccagaatcc ggagtcgccg
    14461 gtgctggtgc tgtcgcacta cgtcgaggag cgatatgcgg cggacctgat cgcctcggac
    14521 acaaagggat tcggatacct gttgaaggat cgggtggcgg atgtgccggc gttcctggac
    14581 gccgtcgaag tggtcggcgg cggcggcacc gtgctggacc cagaggtggt gtcgcagatc
    14641 ctggtgcgct cgcatcggcg cagcgcgctc gacgagctga cgccgcgcga gcgcgaggtg
    14701 ctgcagctga tggccgaggg caagacgaac tcggcgatcg cgtcggcgct gtacatctcc
    14761 gtcggctccg cggaaaagca catcgcatcg atcttcacca agttggatct ggcgcccgac
    14821 gagagcgaga accgccgcgt cctggcggta ctgcgcttcc tcgaatcctg aaccgctgaa
    14881 agggttttga ccgtgacaac cattgctccc ccaccaccgg caccagctac cccgccacct
    14941 ccgctctccc ccggcgggcg caccgcgttc cggatagcgc tcatcgccgc cgcggtggtg
    15001 ctggtggtgg ggaccctcgc ctcgctcggc gccgttgcat ggggcgtcgc cagcttccgg
    15061 gtgatcaccg acagcaagcc gctgccctcc ggcatgcgat ccgtggtcgt cgacaccggc
    15121 tcggtgccca tcgcgattcg catcaccacc gaccgtgagg cccgcgagcc acgcgcggat
    15181 ctgcggatgg tgagctcgat gcgtgccggc gcaaacccgt tggtggtcaa cgccgatccc
    15241 gcgggtgcgc gcgtcacgat caacagcggg gggtcgacgt tcctggacct cggccgggcc
    15301 ggggagatca cccttgtgct gccgcctgac ctcgcccgca agctgaaggt gaccgctcag
    15361 caggacacgg gagtggtgct ggcgcaggcg gatatcgacc agttgatcgc ccacaccgac
    15421 acgggggcgg tggttctcag cggcgcggct cggcgtatcg agatccacac cgacaacggc
    15481 gacgtggtca cgcgcgatcc gatcgcggtg tccgaatcgt ttagcgctac caccgacaac
    15541 ggggatatca aggtggactt ctccgaggcc gcaccccgca ccgttgacgt cactaccgat
    15601 aacggcgatg tcgtcatcgc gctgccccag cgcgggccgt atctggtgaa cgtgaactcc
    15661 acagacggtg atacggagat cagggtgccg cagacgaccg accggcagaa tgcggcggcc
    15721 gtcgtcaccg cccggtccga caccggcgac gtgatcatcg acgatctgcg gtagccgact
    15781 ccgggatcag cggcaggccg aacaacgtga ccaagccggc gtccccgaat acgacaatgc
    15841 gtgcgatacc ggctgccgtc acgtcgagca ccgcgacccc gaacgcccgc agcacgccat
    15901 cctcatcacg acggtaggcc gcaacagcgg gctggccgtt ggcggcgatg gtcaccatcc
    15961 gccaatcacc cggtgcgcca accactcccg cgaggtacgg caggcacgtg gcacggccag
    16021 cgaaccaggt gcgcgacccg accatctcca gcgccgcgtc gtggcgcagc gcctgctcca
    16081 gcagcgcggt gtcagcgttt tcgaacgcgg tcatgtactg atcgagtagc tcgcgggccc
    16141 gcggttccgt cggctcgatg acgtcgtcga cactcggcgc gatgtcctgc atcctggcgc
    16201 gggcccgctg cagcgcgctc ttcaccgcgg cgacgctgat ctcgagcatc ccggccacct
    16261 cggcggccgg gaacgccaac acgtcgcgca gcagaagtac cgcgcgctgc tgagcaggca
    16321 ggtgctgcag gctcgcaatc aacgccagcc gcaggctctc tcgcgacgtg acgacgacag
    16381 cggggtcatc gacccaggaa tccggaatga cttgcagcca caccacattc ggcgcggact
    16441 cggagtcgac atgcggatcg tcagccggcc cgcccaggcc ggacggcagc atgcggcggc
    16501 tgccgtgccc aagcgcggtc aagcacgtat tggtcgcgat gcgatacagc caggtccgca
    16561 ccgacgagcg gttctcgaac tgctggtatc cgcgccacgc ccgcagatac gtctcctgca
    16621 cgacgtcctc ggcgtcgtgc gcggacccga gcatgcggta gcagtgcgcg agcagttcgc
    16681 gtcggtacgg ttcggtgtcg cggacgaagt cgctcatgcc gcggccggcg ggaactggta
    16741 gaccttctcc ggcgtgatcc gcacgatcaa ccgctcagcg tccggctcag gcggaggcgc
    16801 cataccgccc atgtagcggt cgtacatttc gtatagcaat cgcttctcgg gatcgtcggt
    16861 gatctctacg gtgccacgga tctcgatgta gcgccccggc tctttgtcga tcacgagcag
    16921 gctgatccga gggtcgcgcg ccatgttgcg cgtcttcagg cggcccttga tagtgctgaa
    16981 taccacggtg tcgccgtcgc gtttgacgaa gatcaccgac gattgcgggc gcccgtcggc
    17041 attcgatgtc gcgaccacag cgagatgtgg cccgtcgagc acctggcgcg cggtgtcgct
    17101 gaaagttatg gacatgcctg tactgaccag gcggcgcgcg aaaaggaatc ggtcagcggg
    17161 tgtgcgagcg tcctaatcct cgtcagaagc ctgtcctgcg cgggcaccga tcgaaggctc
    17221 gctcggtctc tcgacgattg ttggcggtgg tgcggcctcc tgagcccgca gtgcgtgacg
    17281 tgatttcatg ctcatctctt tgggcagacc cttccggatg cgtcgccgga tgatcccgca
    17341 cgcagctcgc aaccctggaa tccacagctt ttgcgcaaag tttctaccct gcatctcgtc
    17401 gcgggaccag tcgggcgcga gcacgaccct taaccaaaat ccggccgtcc cgaattgatg
    17461 ggacagcgtc cataacgtgt cctcaagctt catcagggat ctgccatcgt ctctggacgc
    17521 cataccatcg atcagctctc tggcaggtag cttgaaggcc ttgcgggcgt tgtcgatgac
    17581 ctcggccgcg ctctggcctt cgttcttcag aatctcctcg atcagcgctt tcctgagccg
    17641 atctgcttcg tcttcgttga cgcctgcggc cgccaacagt tgccgactca gttcacttgc
    17701 cccatcgagc cgtccccaca gccagtcgtt gttgcgaccc tcttcgctgg caaatgcgcc
    17761 gaaatggccc agccgctggc cataaagctt cttcaacggc cacgaaggct gattccccaa
    17821 ccactccagc gggattcccg gcggaacgtc cacgccgggc gtcacgttgt ggaagcggaa
    17881 ctcggggaca tcgtaatcgt cgctacccca agccaacatc ccgctgagca cctctacacc
    17941 gagcgacagc ttcaccaggt tcgagagaga ttcctgttgg aattcagtct gaatggcgcc
    18001 gacggcggta gtcatacaga tgcccagtcg agtcgatagc gagccggccc ctggaccata
    18061 tatcgccgtc aactcgtcgg aaccggtctc gtacaaccga cggagacggg cgcgctgggc
    18121 cgcgggcgat ttgtcgtccg ttggtccacc ccgtgcgacg acgtcgagca cgtcgcacaa
    18181 cccggccacc tcgcgttggc agttggcgac gatctgcatc gccgtcttga tctgcccgcg
    18241 acgttcctgg tccggcagcg cctccaggcg cgccatcgct cggccccacc agcgcagcac
    18301 acggtcggcg acgggccagc cccagtccca cgtattgccc tcaggcgccg cgaatggcag
    18361 ccgcggcata cccggtacgt catcgggctc aagggtggac ggatgcggtg gctgaaacgg
    18421 cgacgttcgg tcaccagcca ccagcatctg ttgacgctcg aaggcctccg ctcggctctc
    18481 ccgatacggc tcgaacacgt gcttcgcggc ttcgatgatc tcgttagcgt cggaaccttc
    18541 gggatccctg aggaactcgt agatcacata gtgcgcctgc gatcgcgaat agctcatctg
    18601 cgtgaagatt ttcctcagag aatcggcgtc caaccgctca tcgggttcgc ggattgccgc
    18661 cagcacagac gtgaaagtct ctcgcagtga cggcgtgcct tccgccttgg atgccgctcc
    18721 ttccggaagt ccaacgccgg gggtcacgta gagcattacc cgttcgtacg gctcatcggt
    18781 cggacgctcc cttagtgctt cgagtagtgg ctcgaacggc gcgttgtcca gcacgccgcc
    18841 gtcgacaagc caactctcgt caataccagg gtctttcggc agtacccgct tcttggccag
    18901 ttccgggagc tcccacaccg gggcgaacgc gcccgggaaa gatgcagacg cgcgcgcggc
    18961 ccgcaccaat acgggtgtga gaacgccgtc tctttcgaaa tcgtctcgga tgtgcggagc
    19021 agcgtccgcg atctcggggc ccagtttgcg ctcgaatcgg tacacgcgcc gcccgtcgcg
    19081 aagtgatatc tctgctccgg tctgcagcgg cgtccgtatc ggagtgctgt cgagggcggt
    19141 agccgtgacg agcagggtgc attccctctt atccttctcg tcttttggcg cgatgctgtc
    19201 gacgatggag cggacttggt cttcgaaata gggaccattc aagacagaat cagctttcga
    19261 ggggtctggc cagaccaaag cgccggcgtg cagcgacgca accgtcgccc attttgactt
    19321 catgtccggc agatcggagc ctcgcgcaac ggctaccgcg agcaaagtgc cattcagacc
    19381 gcctgcgctg gtacctgcta tcagatccac cacggctgtg cgattgcagg cctttagaat
    19441 tttttcccag ggtcgaaggt tgcccttggc gccctggccg cctttccacc cgagtgacgc
    19501 caagcgaatc cgattgatct cgtgtgtaac gccacccatc caaacggcga ggctcacacc
    19561 gccattgagg acaactgcgt atcgaacttg cctagcgttg tccgcttccg cattacccgg
    19621 gtcccctgac acgacgcccc cttattccac ggcaaagtaa gagatcgaat agcgacgtcg
    19681 tatcacactg ggagcaacga aatgcggata agtgccgaga ttgtggcaaa cgaaatatca
    19741 gaggtcgtgc agtccgccga ccgcccggtt cagcgcagct gcggcgcggt cgaccgccgc
    19801 gaggtcttcg acatccgcgg ctttgcgcag gtcatgcatc gccgcatcga ccggtgcagc
    19861 cgggtcgacc ccatgccggg tgcgttccca cacccgctcc agcgcggcca catcagcgag
    19921 caccagtgcc gcgtccctcg ccgccacatc gatcggcagt tgacgcgtcc acaacttcag
    19981 ccgcgccagg tccacgtcga tgacagcttc gtacagcagc cgcagatcca gctcgttctg
    20041 cgcgacgcgc aaggccgccg actgtacatc c
//
LOCUS       DASRJA010000581        27749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2851878,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000581 DASRJA010000000
VERSION     DASRJA010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 27749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27749
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2851878"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VFC01_25690"
     CDS             complement(<1..622)
                     /locus_tag="VFC01_25690"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN83042.1"
                     /translation="MTEFVDVIIVGAGISGISAAWHLQDRCPEKSYVVLERRENLGGT
                     WDLFKYPGIRSDSDMFTLGFRFKPWTSEKAIADGPSIMAYLKETVAEYGIDKHIRYGH
                     KVVGADWSDADNMWTVRVERDGKEVELKSSFLFACSGYYNYDEGYTPEFAGSDDYEGT
                     IIHPQHWPEELDYADKKIVVIGSGATAVTLIPALANSGAGHVTMLQR"
     gene            699..1346
                     /locus_tag="VFC01_25695"
     CDS             699..1346
                     /locus_tag="VFC01_25695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN83043.1"
                     /translation="MTSASQTRHIHGRRAVRPSGDDRELAILETAERLLEERGLAEIS
                     VDDLAKGAGLSRPTFYFYFPSKDAVLLTLLDRVVTEADQKADDALGGTASEAVDPAGV
                     WRSINALFETFGSHRAVTLAGAAARPNNAEVQTMWSRFMQKWIDCTTASIEAERGRGA
                     APTTIPAEDLSVSLNLMNERAMLAAFADEKPAVAYERIVDTLAHIWVTSIYGQSR"
     gene            1371..2600
                     /gene="dinB"
                     /locus_tag="VFC01_25700"
     CDS             1371..2600
                     /gene="dinB"
                     /locus_tag="VFC01_25700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZN83044.1"
                     /translation="MFVSRVGSTSRSSGADILHADLDSFYASVEQRDDPSLRGRPVIV
                     GGGVVLAASYEAKAYGVRTAMGGRQARALCPHAIVVPPRMSAYSQASDAVFEVFRDTT
                     PVVEPLSVDEAFLDVSGLWRVSGTPVEIAAQLRAQVRDRVGLPITVGIARTKFLAKVA
                     SQEAKPDGLLLVPPDRELAFLHPLPVRRLWGVGVKTAEKLHAHGIETVADVAELGEST
                     LAAMVGGAMGRQLFALSRNIDRRRVVTGVRRRSVGAQRALGRAGNTMSAAEVDAVVVN
                     LIDRITRRMRKASRTGRTVVLRLRFDDFGRATRSHTMARATASTDVILETARSLVAAA
                     APLIGERGLTLVGFAVSNIDREGAQQLELPFEEHADTIAIDDAVDKVRQRYGNTALTR
                     GVLLGRDPGLEMPHLPD"
     gene            complement(2607..5846)
                     /locus_tag="VFC01_25705"
     CDS             complement(2607..5846)
                     /locus_tag="VFC01_25705"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZN83045.1"
                     /translation="MGEHTGDKPDTKTDETTSEETGTETESEPTDEPTSPRGGEVKPA
                     EPEAATKHVETEAKSNDDPTRVAARPVPARDPEPIARAAKAAPVETAEPLSPRSTVAT
                     ANPQRVAVQALSATTAMPAAPTAAAVPVPAPTVARTPVGPLTSLLALFGLQPAAPAAP
                     VTPLSQLVELIWVAVRRIEHTFFNQGPRATTPMLISHDLETGAITGKVGVVDPDPEDR
                     LTYTVVDNPDHGTVAVSADGVYTYTPDKDWAHATPGTDTFTVRVSDAVNGFHLHLFSP
                     TGDTVTVPITVQVAAINKAPTITATPTTPNQATGAITYTVVARDPENDAVTVTVSQPV
                     HGAIVRNTDGTYTYTPDRDFAHGLSTPGSAQAADVITFTATDRYNAATSVTVNASITP
                     LNDAPEINAAVTGVNTAGDVTIQLSVTDDNRDRVKITLPDPAHGSFTTLTVTDAEGDT
                     TTVTSPQPGLQLELSDTDVVTIVYRPDRVMGAPDTEDLTFVGNDGHGGAAVATVTATI
                     AIPNRAPVITVGAPTVVNAATGAVRYPVTVSDPDGDPFTLTVSDPANGDIVRNADGSY
                     TYTPDPTFAHRLSLGGHTDPAADAINFVAADQLGATAITVVFASITPQNTAPVTYANT
                     SVGDPDTNGTVTGRVFVRDNENDGITYAITSTKGTVTIDNNGNFTFDPSTQARQQAAA
                     IGASAEDKQAIVVITANDGHGGQLRLEVTVDVLPATGNTITFADGARPYEVFVPSNDA
                     TKIYVWTGPQLRIVDTTTRTLVGTIDFAGNPSAVVMSQDGRYFYFAKTTTGTEFGPVT
                     KVDLQTGAQTPIQGVVRQPSAMAISSDGATLYVTNRIDGTVSVINTATGAFYTIDTGL
                     QSDAIAVSADGGTLYVGSIINDVRVVNIAARTYTVLPTGTYDGHSVATQRITVAGRYV
                     YVTDTLNDKVAVIDSNDNTVVARVNVGDGPSSIAASPGGAFVFVANGQSDSVSVIDTD
                     TGTVATTIPVGDNPTNVDLSADGLRLYVSTRAGIQIVPIADIYYLFGGGNDDGPGWAV
                     GAGTGTGTDPSVPPVTGGGGLGGGGTGPVVPPIIPGGPGPIRPPGEIRA"
     gene            5857..6099
                     /locus_tag="VFC01_25710"
     CDS             5857..6099
                     /locus_tag="VFC01_25710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83046.1"
                     /translation="MVSLPVDDADEGADEVESDEESEDSDDFGEGPLVSDGSAEATPW
                     PVDTATPRPIAKVSPLRRDAFFGVTMTGLLCLGTAS"
     gene            6597..7415
                     /locus_tag="VFC01_25715"
     CDS             6597..7415
                     /locus_tag="VFC01_25715"
                     /inference="COORDINATES: protein motif:HMM:NF039175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EspA/EspE family type VII secretion system
                     effector"
                     /protein_id="HZN83047.1"
                     /translation="MSLLGEALDLLRRYGDVVGIDWSKPPGLLDKAANTLIDIAGSPI
                     LGGAQAVIHGMKATTGSGEPEAGEAFKKSADLYDEAGSLLIDAAVKPDRWDGTAAQAY
                     EAKNDAHRRLTIGVADAEKNMQSYLSELAAQVTETRTNLDDRIKFLSDYDTATSWMNA
                     IPGGAVVKAAADLAVASTQLEMAKFSMAKLLAESALNANRIRDQLATYGDAAGEALFA
                     DDKNLPCGEPFGDERTQGQLPQRINPTQPYEIPEPQGPPVIYPPAKPADTPAPR"
     gene            7447..7767
                     /locus_tag="VFC01_25720"
     CDS             7447..7767
                     /locus_tag="VFC01_25720"
                     /inference="COORDINATES: protein motif:HMM:NF022280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESX-1 secretion-associated protein"
                     /protein_id="HZN83048.1"
                     /translation="MTAQEDNLRVLTDHIDDIAAKQTTAAGTLKIAGETVNDIASSVW
                     STHGVVCAASNMAVDAVETARDGARTKLWQMSHDLSERLHTASENYNNADWLAGRDID
                     SCRL"
     gene            7798..8262
                     /locus_tag="VFC01_25725"
     CDS             7798..8262
                     /locus_tag="VFC01_25725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family DNA-binding protein"
                     /protein_id="HZN83049.1"
                     /translation="MTDDATRRAAWEDGDDDRDGGASALDALGEYSPVSEEEADQVLA
                     ALFTVTNPARTVSATAAIGGRLQQIALSANATDMTEAELAEEICVLADLARQKAQAAQ
                     HAVIVQQMRSMGHDSVMTSGFLERDMDLPSPETVEARRAEVFAARYPFDDEA"
     gene            8273..9637
                     /locus_tag="VFC01_25730"
     CDS             8273..9637
                     /locus_tag="VFC01_25730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83050.1"
                     /translation="MRRFLIAIAVISAVVAVTAAVVWWIQDDGSAAARRAWQPPPSPV
                     FSSPMRVQPVAGWRMSTTDLGLPPPVDGMEPGRIGTDNDPFRSRPFIGNLDDHAYFLA
                     STPATPGPQWWMIGVDVRSGKRLFAPIQLGATTLPPECFLNGRANMLCINDESHTAYV
                     VELRSGTVTHTGPTDLRLGYGKLAVEQVGIYAVAKTQDQGVYGIGPRAETTWFVPGDG
                     LIRPITETGFETTPQTLGAQLSTDARAYDMTVFSVSDGKVVKPEIHHDAHLGETEIYP
                     GGFAAAIETKPKRLEVHFFDDTGKRLSDRAVHGVVRESIGLPVVASDDQSTIYSPAGK
                     KLLDLPEGATRLVGSTLFVNENDSDAFPMWRQYDLETGDKGPACDFSMSNFLGTNGSV
                     IVFEVRNPKAEIVAKARDLATCDTLWTLPKRVDSFAKVWRVNTTLVQLSDDGTELFSL
                     VAPS"
     gene            complement(9634..10638)
                     /locus_tag="VFC01_25735"
     CDS             complement(9634..10638)
                     /locus_tag="VFC01_25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZN83051.1"
                     /translation="MDPVIALRQIAYYKDRAREDSRRVMAYRNAADIVEGLTEAERER
                     HGAANSWQSLPGIGPKTAKVIAQAWAGQEPDALVELRSNVKDLGGGDIRAALKGDLHV
                     HSNWSDGSAPIDEMMLAARELGHEYCALTDHSPRLTIANGLSPERLRKQLDVIDELQD
                     AVAPMRILTGIEVDILEDGSLDQEEELLKRLDVVVASVHSKLSMDAPSMTRRMLKAVQ
                     NPHADVLGHCTGRLVTGGRGIRPESKFDAEKVFTACRDHGTAVEINSRPERRDPPTRL
                     LKLALEIGCLFSIDTDSHAPGQLEFLGYGAQRAVENAVPVERIVNTWSVDKLLEWTAS
                     "
     gene            complement(10656..11231)
                     /locus_tag="VFC01_25740"
     CDS             complement(10656..11231)
                     /locus_tag="VFC01_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HZN83052.1"
                     /translation="MIDFTPACQRTAAVLANITDDKLTAATPCADYSVGHVLHHVGGL
                     APAFAAAARKDRGPLTGTPPSVDAAQLDPDWRASYPTRLAELASAWTDPSAWQGMTRI
                     AAMDMPGEAVAMVGLTEVVIHGWDLARATGQPYDIEPDIAEAVLANVAEFAAQGPVDG
                     LFGAAVPVADDAPVLDRIVALSGRDPGWHSP"
     gene            complement(11228..11935)
                     /locus_tag="VFC01_25745"
     CDS             complement(11228..11935)
                     /locus_tag="VFC01_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HZN83053.1"
                     /translation="MRIAIAGAGAVGRSVAQELLDYGHKVLLIEHNVGHYEPHTVPDA
                     DWLLADACELSSLEASGMQTCDVVIAATGDDKVNLAAALLAKTEFGVARVVARVNDMR
                     NEWLFTEAWGVDVAVCTPRAMMASIEGAIDIGHLVQLMELRQGQVSMAKLTLPEDNPL
                     VGTRVGDLALPENTALAIMVRDGEPIVPKPDDVFEAGDEMLFFTGGAAENQTRALVRG
                     AVARSDELPSRGRSVET"
     gene            12073..13569
                     /locus_tag="VFC01_25750"
     CDS             12073..13569
                     /locus_tag="VFC01_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HZN83054.1"
                     /translation="MTLQQLYLVLLGGGLVLLASIVATRAASRVGLPSLLLFLGVGVI
                     VGEDGLGLHFDNYLLADHLGTVALAIILVEGGLTTRFGDVRKVLAPAAVLATVGIAVS
                     MAVTAVGAHLLLGIDWQLALLLGAVVSSTDAAAVFSVLRVVPLPRRVAGLLEAESGFN
                     DAPAVILVLMFSVVPLELSPLHALAELAYELSVGAAIGLGCGFLGALALRRIALPASG
                     LYPLATFGLGMVAFAAAGSAHASGFLAAYLAAVVLANSGLPHRSATRSFAEGLGWPAQ
                     IGLFVLLGLLVDPSDLGAELVPGIVIGLVLLLVARPVSVLVSLAGFRVPWREQVFLSW
                     AGLRGAVPIVLTTFPIVGGVPDSMRLLNIVFILVVVYTLVQGPTLHPLAHRLGLIPKE
                     ATREIQVEAAPLDVLDAELLTMTVHAPSRLHNVTVLELRLPDPSVITLIIRNGHTFVP
                     QPDTRIAIGDELLIVTTVKTRAAAERRLRAVSRRGKLAHWFDEYGEAD"
     gene            13609..14496
                     /locus_tag="VFC01_25755"
     CDS             13609..14496
                     /locus_tag="VFC01_25755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN83055.1"
                     /translation="MRFAFKTSPQNTTWSDMLAVWQAADDIDVFESGWTFDHFYPIFS
                     DSSGPCLEGWTTLTALAQATKRLRLGTLVTGIHYRHPAVLANMAAALDIISDGRLELG
                     IGAGWNEEESGAYGIELGSIKERFDRFEEACEVLTSLLSKDTTTFDGGFYQLKDARNE
                     PKGPQKPHPPICIGGSGEKRTLRITAKYAQHWNFVGGPPEEFARKRDVLASHCADIGR
                     DPKEIMLSAHVRLSPDHHNPDSPTDFSHAIDEAAALGKEGLDLAIVYLPTPHTPSVLE
                     PLAEAIRDSGLLTSKDNGR"
     gene            complement(14884..15702)
                     /locus_tag="VFC01_25760"
     CDS             complement(14884..15702)
                     /locus_tag="VFC01_25760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZN83056.1"
                     /translation="MTGRRPITYAGTELNRRHFLAGLSLATLAGVGLARCTVGAQPRL
                     AQAAAAPIAPPVVAVRMPLPGGGALSSIPGGGNLLALTLDDGVSSDVVRLYTEFAKDT
                     GVRLTYFVNGTYRSWTENRNLLWPLVESGQIQLGNHTWSHPDLAILPKDQVADQLRRN
                     HQFLMRNYGVDARPYFRPPYGSHNPVVDAVAAELGYTMPTMWSGSLEDQNVIGEDEIV
                     KMANKYFNPRAIVIGHLNHLPVTHVYGQLIDVIRSRNLQTVTLDDVFVKPERQT"
     gene            complement(15783..17249)
                     /locus_tag="VFC01_25765"
     CDS             complement(15783..17249)
                     /locus_tag="VFC01_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HZN83057.1"
                     /translation="MADPKGFLKYTQRETPQRRPVPLRLMDWKEVYEDFSHDTLREQA
                     ARCMDCGIPFCHNGCPLGNLIPEWNDLVRSDRWRDAIERLHATNNFPEFTGRLCPAPC
                     EGSCVLGINQDPVTIKQVEVEIIDNAFDNGWVVPLPPDKRTGKTVAVVGSGPAGLAAA
                     QQLTRAGHTVTVFERDDRIGGLLRYGIPEFKMEKRHIDRRLTQMEAEGTVFRAGVNVG
                     VDITAAQLRADFDAVVLAGGATAWRDLPAPGRELDGIHQAMEFLPWANRVQHGDPVTD
                     DNGLPPIHANGKKVIIIGGGDTGADCLGTVHRQGAASVHQFEIMPRPPETRADSTPWP
                     VYPLMFRVSSAHEEGGERVYSVNTEEFSGSDGHVTGLRGHEVVMNAGKFEKVEGSDFE
                     MEADLVLLAMGFVGPEREGLLTDLGVEITDRGNVARDANFATTVPGVYVAGDMGRGQS
                     LIVWAIAEGRAAAAGVDRYLMGQTALPAPIKPTAVPQR"
     gene            complement(17242..21822)
                     /gene="gltB"
                     /locus_tag="VFC01_25770"
     CDS             complement(17242..21822)
                     /gene="gltB"
                     /locus_tag="VFC01_25770"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HZN83058.1"
                     /translation="MTPRIQGLYNPAFEHDACGVAMVADMHGRRTRDIVDKAITALVN
                     LEHRGAQGAEPHTGDGAGILLQVPDEFLRAVVEFDLPEYGSYATGMAFLPQSSKDAAA
                     ACDAVEKIAEAEGLEVLGWREVPTDESSLGALARDAMPTFRQVFMAGASGMDLERRTY
                     VVRKRAEHELGTKGPGQDGPGRETVYFPSLSGQTFVYKGMLTTPQLKAFYLDLQDDRL
                     TSALGIVHSRFSTNTFPSWPLAHPFRRIAHNGEINTVTGNENWMRAREALINTDVFGG
                     EDLEKVVPVCTPGASDTARFDEVLELLHLGGRSLPHAVLMMIPEAWERHESMDPARRA
                     FYQYHASLMEPWDGPASMTFTDGTVIGAVLDRNGLRPSRIWVTNDGLVVMASEAGVLD
                     LDPSTVVKKMRLQPGRMFLVDTSKGRIVADEEIKAELAAELPYQEWLDAGLFHLDELP
                     PGDYVRMPHHRVVLRQQIFGYTYEELNLLVAPMARSGVEALGSMGTDTPIAVLSQRPR
                     MLYDYFQQLFAQVTNPPLDAIREEVVTSLSGTVGPEGDLLNPTAESCRQIVLPQPILR
                     NAELSKLICVDPDHEIRGHKHGMRAAVIRCLYPVNRGGQGLKEALDNVRAKVSAAIRD
                     GARIIVLSDRESDESMAPIPSLLSVSAVHHHLVRDRTRTKVGLVVEAGDAREVHHMAC
                     LVGFGAAAINPYMAFESIEDMVDRGVITGISSDKAKANYVKAAGKGVLKVMSKMGIST
                     LASYTGAQLFQAIGISQQMLDEYFTGLACPTGGIDLDDIADDVAARHALAYLDRPDEW
                     AHRELEVGGEYQWRREGEYHLFNPDTVFKLQHSTRTGQYSIFKEYTKLVDDQSERMAS
                     LRGLLKLKDGVRAPVPLDEVEPASEIVKRFSTGAMSYGSISAEAHETLAIAMNRLGGR
                     SNSGEGGEHVSRFDRDENGDWRRSAIKQVASARFGVTSHYLTNCTDIQIKMAQGAKPG
                     EGGQLPGNKVYPWVAEVRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANPQARVH
                     VKLVSENGVGTVAAGVSKAHADVVLISGHDGGTGATPLTSQKHAGAPWELGLAETQQT
                     LLLNGLRDRIVVQVDGQLKTGRDVVIAALLGAEEFGFATAPLVVSGCIMMRVCHLDTC
                     PVGVATQNPTLRARFNGKPEFVENFFMFIAEEVREMMAQLGFRTVNEMVGQVGSLDTT
                     RAAEHWKAYKLDLAPVLYEPESAFMNQDLYCSSRQDHGLDKALDQQLIVMSREALDSG
                     TPVRFSTTIANTNRTVGTMLGHELTKAYGGQGLPDGTIDITFDGSAGNSFGAFVPKGI
                     TLRVYGDANDYVGKGLSGGRIVVRPSDNAPADYVAEQNIIAGNVILFGATSGQVFLRG
                     VVGERFAVRNSGAHAVVEGVGDHGCEYMTGGKVVILGPTGRNFAAGMSGGVAYVYDPA
                     GALGDNLNAEMVDLDEMDDDDVEWLRGMIQAHVDATDSAVGQRVLSDWENELKNFVKV
                     MPRDFKRVLVAIAEAERTGSDVDEAIMAAANG"
     gene            complement(22220..24262)
                     /locus_tag="VFC01_25775"
     CDS             complement(22220..24262)
                     /locus_tag="VFC01_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZN83059.1"
                     /translation="MTEVAHTQHQVHADHAEHAHAGHGDHVAQFRRLFWTMLVLAVPT
                     VLLSGMFAMILGYSVPVFPGSQWVSPLLGTVMYVWGGRPFLTGAISEIRSRAPGMMLL
                     IGLAITVAFVSSWGASVGVLPHNLDFWWELALLIVIMLLGHWIEMRSLAQTTSALDSL
                     AALLPDEAERVTPNGTETVAPSELRVDDVVIVRPGGSVPADGVILDGAADIDESMVTG
                     ESRPVRRSQGDAVVAGTVATDSGLRVKVTAVGDDTALAGIQRLVAEAQNSSSRAQRLA
                     DRAAAWLFWFALGAAIVTAVVWGFIGQPEEAVIRTITVLVIACPHALGLAIPLVVSIA
                     TERAARGGVLIKDRLALESMRTVGAVLFDKTGTLTKGEPTVTAVATTDGRTEDDVLAI
                     AAAAESDSEHPLAKAIVDAARRRGLTVPGSSDFTSSPAVGVTARVDGTIVRVGGPRLL
                     EEAGRNELPEARPWREQGAIILHVLVNGTVVGALALADEIREESRQAVDALHKLGIQV
                     VMITGDARAVAESVAAELGIDRVFAGVRPEDKAAKVAQLQDEGLKVAMVGDGVNDAPA
                     LAQADVGIAIGAGTDVAIASAGVILASSDPRSVLSVIQLSKASYRKMKQNLWWAAGYN
                     LISVPLAAGVLAPIGFVMPMSVGAILMSISTVVVALNAQLLRRLDLRPEASVQALL"
     gene            24375..25712
                     /locus_tag="VFC01_25780"
     CDS             24375..25712
                     /locus_tag="VFC01_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HZN83060.1"
                     /translation="MTAPAAGPDQPDDSMRGSVHICVTAIVAGVLIGFVGGAFRWCLQ
                     KADELRIDLVDWAHGLPGPGWLVPMAAAAAGATLAALIVRWEPLAAGSGIQHVEAVFL
                     GEARPPLIRLVPAKFIGGVLSMGSGLVLGREGPTVHMGAAIGAEAARRARLPDSEIRM
                     MQTALGGAGLAVAFNAPIGGTLFTLEEVTKSFRVKTVLATLFSAAAAVACSRLILGNH
                     ADFQVEPIDAPALAWLPLFVVFGLLTGCLGAAYNWLVLWFLDHVGAIRRIPALAKAAI
                     IGAVIGFAMFVYPLAVGGGEGLTQRILGGGHIVLAVVIGILAVRFIAGPLSYSAAVPG
                     GLFAPLLAVGALWGLLFAGGFNAAWPENATQLAVPMALVGMAAFFGATVRAPVTGMVV
                     VTEMTATTETLVPMMAATAAAVFAAYVVGSPPIYESLRERMPDETSIEERGRG"
     gene            25722..26771
                     /locus_tag="VFC01_25785"
     CDS             25722..26771
                     /locus_tag="VFC01_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN83061.1"
                     /translation="MPTTHATSLTNGEIVEENDLGSIRRVTADSLPILSGMSIKRIVV
                     NPGAMRTPHWHANCNELTYCVSGTSFVSVLDSYSKFSSFTISAGEMFHIDSGSLHHIE
                     NIGEEPAEFILAFRSERPEDFGLAGSFGAMTDAVLGNTYDLPASDFAKMRRDTNDRRL
                     AARKADAVVPSTAYFDDPHKFGVEAQNPVIGIAVGSARLARAQYWPALKDLSMYSLRI
                     REDGMREPHWHPVTAEMGYVHKGSGRMTVMDPDGSLDTYYLKEGDVYFIPRAYPHHIE
                     VVDAPDIHFAIFFDQPMPGDIGYRASASAYSREVLAATFNMHIDDLPNFPFTKTDPLI
                     VTRNNPVDEHAMAER"
     gene            26889..27662
                     /gene="aqpZ"
                     /locus_tag="VFC01_25790"
     CDS             26889..27662
                     /gene="aqpZ"
                     /locus_tag="VFC01_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HZN83062.1"
                     /translation="MREPTMLHRLAAEFIGTFWLVFGGCGSAVFAAKFTSDGTSLGIG
                     FLGVALAFGLTVLTGVYAFGTISGGHFNPAVTLGAALARRVEWRVLPAYWITQVVAGL
                     GAGMMIYVVAKGQEGWTATGHMAANGFGAHSPGGYSLWAVIVAEVVLTGVFLLVILGS
                     TDDRAPKGFAGLSIGLTLTLIHLISIPISNTSVNPARSTGVAFFNGDGAPAQLWVFWL
                     APLLGAAIAGIAYPYLFGRNEELADRPVRDNALEEPATT"
BASE COUNT         4649 a         9024 c         9074 g         5002 t
ORIGIN      
        1 agcgctgcag catcgtgacg tgcccggcac ccgaattggc aagcgccgga atcagagtca
       61 ccgcggtggc gccgctgccg atgaccacga tcttcttgtc cgcgtagtcc agctcctccg
      121 gccagtgctg cgggtgaatg atcgtgccct cgtagtcgtc cgaacctgcg aactcgggtg
      181 tgtaaccctc gtcgtagttg tagtagccgc tgcaggcgaa caggaaggag ctcttcagct
      241 cgacctcctt gccgtcgcgt tcgacgcgaa cggtccacat gttgtctgcg tcggaccaat
      301 ccgcgccgac caccttgtgg ccataccgga tgtgcttgtc gatgccgtac tcggcgaccg
      361 tctccttcag gtaggccatg atcgacggtc cgtcggcgat ggccttctcc gacgtccacg
      421 gcttgaaccg gaagccgagg gtgaacatgt cggagtccga gcggatgccc gggtacttga
      481 acaggtccca ggtgccgccg agattctccc ggcgctccag gaccacatag ctcttctcgg
      541 ggcagcggtc ctggaggtgc caggccgcgc tgatgccgga gatgccggct ccgacgatga
      601 tgacgtcgac aaattcggtc atgatgtaaa gctatcaacg cggtgtcgaa cgagtcaaca
      661 gtgtgtcgag aaattcgaca tcatgtaaag tcaacgccgt gaccagcgcc agccagaccc
      721 gccatatcca cggtcgccgc gcggtgcgac cgtctggcga cgaccgagag ctggccatcc
      781 ttgagaccgc cgagcgcctc ctagaggagc gtggcctggc cgagatctcc gtcgacgacc
      841 tggcgaaggg cgctggcctt tcccggccga ccttctattt ctatttcccg tccaaggacg
      901 cggtgctgct gacgctgctc gaccgggtcg tcaccgaggc ggaccagaag gccgacgatg
      961 ccctgggcgg gacagccagc gaagccgtcg accccgctgg ggtgtggcgg tcgatcaatg
     1021 cgctgttcga gaccttcggc tcccatcgcg cggtgaccct cgccggcgcg gctgctcggc
     1081 cgaacaacgc agaggtacag acgatgtggt cgcgcttcat gcaaaagtgg atcgactgca
     1141 ccaccgcatc gatcgaggcg gaacgcggac gaggcgccgc tccgacgacg attccggccg
     1201 aggatctgtc ggtctcgctc aacctgatga acgagcgtgc aatgcttgcg gcattcgcgg
     1261 acgagaagcc ggcggtggcc tacgagcgca tcgtcgacac tctcgcccac atctgggtca
     1321 ccagcatcta cggccagtca cgctgattcg tcggaccccg atgcgaacat atgttcgtgt
     1381 cgcgggttgg atcgacgtct cggtcgtcgg gcgccgacat cctgcacgct gacctcgatt
     1441 cgttctatgc atcggtcgag cagcgcgacg acccgtcgct gcggggcagg ccggtgatcg
     1501 tcggcggcgg tgtcgtgctg gcggccagtt atgaagccaa ggcgtacggg gtgcgcaccg
     1561 cgatgggtgg ccgccaggcc cgcgcgctgt gcccacacgc gatcgtggtg ccgccgcgga
     1621 tgtcggcgta ttcgcaagcc agcgacgcgg tattcgaggt gttccgcgac accacgccgg
     1681 tagtagagcc gctctcggtg gatgaggcct tcctcgacgt gtcggggctg tggcgggtat
     1741 cgggcactcc ggtcgagatc gccgcacaac tgcgcgcgca ggtgcgcgat cgcgtcgggc
     1801 tgcccatcac ggtcggcatc gcgcgcacca agttcctagc caaggtcgcc agtcaggaag
     1861 ccaagcccga cggcctgctg ctcgtgccgc cggaccgtga gctggctttc ctgcatccct
     1921 taccggtgcg ccggttgtgg ggcgtcggtg ttaagacggc cgagaaacta cacgcgcacg
     1981 gcatcgagac tgtcgccgac gtcgccgaac ttggcgagtc gacgctcgcc gcgatggtcg
     2041 gcggcgccat gggccgtcag ctatttgcgt tgtcgcgcaa catcgatcgc cgtcgagtgg
     2101 tgactggcgt gcggcgccgc tcagtcggag cgcagcgggc attgggccgc gcgggcaaca
     2161 ctatgtcagc ggcggaggtc gacgccgtcg tcgtcaatct catcgaccgg atcacgcggc
     2221 gaatgcgcaa ggccagccgc accggtcgaa cagtggtgct gcgcttgcgg tttgatgatt
     2281 tcggccgagc gacccggtcc cacacgatgg cccgggcgac ggcgtcgacc gacgtcatcc
     2341 tcgaaaccgc acgcagcctg gtcgcggcgg cggcgccgct gatcggcgaa cgcggtctga
     2401 cgctggtcgg tttcgcggta tccaacatcg accgcgaagg tgcacaacag ctcgagctgc
     2461 cgttcgagga acacgccgac accatcgcga tcgatgacgc ggtcgacaaa gtgcgacagc
     2521 gctacggcaa caccgcgctg acccgcggcg tgctactcgg ccgcgacccc ggcctggaga
     2581 tgccgcatct gccggactga tcgggttcag gcacggattt ctcccggcgg ccgaatcgga
     2641 ccggggccac ccggaattat cggcggcaca acgggtcccg tgccgccgcc gccgagcccg
     2701 ccgcccccag tcaccggggg cacggaggga tcggtgccgg ttcccgttcc ggctcccaca
     2761 gcccacccgg gtccgtcatc gttaccacct ccgaagaggt agtagatgtc ggcgatcgga
     2821 acaatctgga ttccggcgcg ggtcgacaca tagaggcgca agccgtccgc gctgaggtca
     2881 acgttggtgg ggttgtctcc gaccgggatc gtggtggcca ctgtcccggt gtcggtgtcg
     2941 atcactgaca cgctgtcact ttgaccgttc gcgacgaaga caaaggcgcc gcccggactg
     3001 gccgcgatac tagacgggcc gtctcccacg ttgaccctcg cgacgacggt gttgtcgttg
     3061 gagtcgatga ctgcgacctt gtcgttgagc gtgtcggtga catacacgta gcggccggcg
     3121 acggtgatcc gctgggtcgc caccgaatgt ccgtcatagg tgccggtggg cagaaccgtg
     3181 taggtacggg ccgcgatgtt gaccacgcga acgtcgttga tgatgctgcc gacgtacagc
     3241 gtgccgccgt cggcgctcac cgcgatggcg tcactctgca agccggtgtc gatggtgtag
     3301 aacgcgccgg ttgccgtgtt gatgaccgat acggtgccgt ctatgcggtt tgtgacatag
     3361 agggtggcgc cgtcggagct gatcgccatc gcggatggtt ggcggactac gccttgaatc
     3421 ggagtctggg caccggtctg caggtcgacc ttggtgaccg gaccgaattc tgtgcccgta
     3481 gttgtctttg cgaagtagaa gtacctgcca tcctggctca tcaccaccgc ggacggattc
     3541 ccggcgaaat cgattgttcc gaccagggtg cgcgttgtgg tgtcgacaat ccgaagctgc
     3601 ggcccggtcc acacgtagat cttggttgcg tcgtttgacg gcacgaagac ctcgtatggt
     3661 ctggcaccgt ccgcgaaggt gatggtgtta ccggtcgcgg gcaggacgtc aacggtgacc
     3721 tccaaccgca actgtccgcc gtggccgtcg ttggcggtga tgacgacgat cgcctgcttg
     3781 tcttccgccg acgcgccgat ggcggcggcc tgttggcgcg cctgcgtgga aggatcgaac
     3841 gtgaagttgc cgttgttgtc gatcgtgaca gttcccttgg tgctcgttat ggcataggtg
     3901 atgccgtcgt tttcgttgtc ccgcacgaag actcgaccgg tgacggtgcc gttcgtatcg
     3961 ggatcgccga cgctggtgtt tgcgtacgtg accggcgcgg tgttctgcgg cgtgatcgaa
     4021 gcgaagacaa ccgttatcgc tgtggcgccg agctgatcgg ccgcgacaaa gttgatggcg
     4081 tcggctgcgg gatcagtgtg tccacccagg ctcaggcggt gcgcgaaggt cggatcggga
     4141 gtgtaggtat agctgccatc ggcgttgcgg acgatgtcgc cattggccgg gtcgctgacg
     4201 gtcagcgtga aggggtcgcc gtcgggatcg ctgacggtga ccggatagcg gacagctccg
     4261 gtggcagcgt tgacgaccgt cggggcgccg accgtgatca caggcgcgcg gttcggtatc
     4321 gcgattgtcg cggtcacagt cgccactgcg gcgccgccgt ggccgtcgtt tcccacgaac
     4381 gtcagatcct cggtatcggg tgcacccatc acgcggtcgg gccggtacac gatggtgacg
     4441 acgtcggtgt cggacaactc gagctgcagt ccgggctggg gtgacgtcac cgtcgttgtg
     4501 tcgccttcgg catcagtgac cgtcagcgtc gtgaagctgc cgtgggccgg atcgggcagc
     4561 gtgattttca cgcggtcccg gttgtcgtcg gtgacggaca actggatggt cacgtcgccg
     4621 gcggtgttga cacctgttac ggcggcgttg atctcagggg cgtcgttaag cggagtgatc
     4681 gacgcgttga cggtcacaga cgtcgccgcg ttgtatcggt cagttgcggt aaaggtgatc
     4741 acgtcagcgg cctgggccga gccaggtgtg ctgaggccgt gcgcgaagtc gcgatcgggc
     4801 gtgtaggtgt atgtgccatc ggtatttcgg acgatggcgc cgtggacggg ctggctgacg
     4861 gtcactgtga cggcgtcgtt ctcggggtcg cgggcgacga cggtgtaggt gatcgctccg
     4921 gtggcctggt ttggggtcgt cggcgtcgcc gtgatggtcg gcgctttgtt gatagcggcc
     4981 acctggaccg tgatcgggac ggtcaccgtg tcaccggtcg gcgagaacag gtgcagatga
     5041 aaaccattca ccgcgtcgct gactcgcacg gtgaaggtat cggtgcccgg cgtggcgtgc
     5101 gcccagtcct tgtctggcgt gtaggtgtag acaccgtcgg cgctgacggc gacggtgccg
     5161 tggtcggggt tgtcgacgac ggtgtatgtc agccggtcct ctggatcggg gtcgactact
     5221 ccgacctttc cggtgattgc accggtttcg agatcgtggc tgatgagcat cggtgttgtc
     5281 gcccttggtc cttggttgaa gaaggtgtgt tcgatgcgcc tcaccgccac ccagatcagc
     5341 tcgacgagtt gtgacagcgg cgtgacgggc gccgccgggg cggcgggctg caacccgaac
     5401 aacgccagca ggctggtcag cggtccgacc ggcgttcggg cgaccgtcgg tgccggcacg
     5461 ggaacggccg cagccgtggg tgccgcgggc attgcagtgg tagccgaaag tgcctgcacg
     5521 gcaacgcgtt gtggattcgc ggttgcaacc gttgaacggg gcgatagcgg ttccgcagtc
     5581 tcgacggggg cggccttcgc tgcgcgggcg atcggttctg gatcccgggc gggaacgggt
     5641 cgggctgcga cccgcgtcgg atcgtcgttc gacttcgcct ccgtctcaac gtgtttggta
     5701 gctgcctctg gctcggcggg cttcacctcg ccgcccctcg gcgacgtcgg ctcgtccgtc
     5761 ggctctgact ccgtctctgt tccggtttct tccgacgttg tctcgtcggt cttggtgtcg
     5821 ggcttgtctc cggtgtgctc acccaccgat gtgtgggtgg tctcgttgcc tgtcgacgac
     5881 gccgacgaag gcgcggacga agtcgaatcc gacgaggagt ctgaggactc cgacgacttc
     5941 ggcgaaggtc cgctggtatc cgatggttcg gccgaagcaa cgccatggcc ggtcgacaca
     6001 gcgacgccca ggcccatcgc caaggtcagc ccgctcaggc gcgatgcgtt cttcggtgtg
     6061 accatgaccg gcctcctctg cctggggacc gctagctgag gcgggttgtc cgcgcaccag
     6121 gtggcccgag cagaaaattt acgcacgcga caatgaggtg cgcggtcgat tcggaaattt
     6181 attttgctga gaatcaccaa ccgaccccag agaaggtcgc tatctttcgt ttcggaaagt
     6241 aataagcacg cgcgaactcg gcgagcttct tctttcctgc cgcatcacca gtcaccgatc
     6301 tgaatcttag cctaaacagg cactacatcg cgttattcct agtcaaagcg ttgcggtaag
     6361 ggcgccatcg atgcgccaga ttctcgcctt gtggaataca gaagatttgc gagtttcaca
     6421 tcatgtgtac acattccatg cgccgtagat atgttcgctg cggatccagg tcaattattg
     6481 ctaatcaaga tcgaagaccg cgaaaccgtc cgctgcagaa acttgtcggg ccgaaattgg
     6541 cgcaatgccc tcacgcggat ctgacttagg cttgatccga cggaagggac cgcgcagtga
     6601 gtttgctggg agaagcgctc gacctgcttc gacgttatgg cgatgtcgtc ggcatcgatt
     6661 ggagcaagcc accgggtttg ctggacaagg ccgcgaacac gctcatcgat attgccggtt
     6721 caccgattct cggcggggcg caggccgtga tccacggcat gaaggcaacc accggctccg
     6781 gcgagcccga agccggtgag gcgttcaaga aatccgcgga cctgtacgac gaggcgggca
     6841 gcctgctcat cgacgccgcg gtgaaaccgg atcgctggga cggcaccgct gcgcaggcct
     6901 acgaggcgaa gaacgacgct caccgacgcc tgaccatcgg ggtggccgac gccgagaaga
     6961 acatgcagag ctacctcagc gagttggcgg cccaagtcac cgagacccgg acaaacctcg
     7021 acgaccggat caagtttctc tccgactacg acaccgcgac gtcgtggatg aacgccattc
     7081 ccggcggagc cgtggtcaag gccgcagccg atctggcagt cgcgtccaca caactggaaa
     7141 tggcgaagtt ctccatggcg aagctgctcg ccgagtccgc gctgaacgca aatcgaattc
     7201 gagatcagct agccacttat ggggacgctg caggggaagc gctattcgca gacgacaaga
     7261 atctgccctg cggcgaaccg ttcggtgatg agcgcacaca aggccagctg ccgcagcgca
     7321 tcaaccccac gcagccgtat gagattcccg agccccaggg accgcccgtg atctatccac
     7381 ccgcaaagcc tgccgataca ccggctcccc ggtgaggcat acagcgaccg aaatggagga
     7441 acagtcttga ccgcccaaga ggacaatctc cgggtattga ccgaccacat cgacgacatc
     7501 gcggccaagc agacgaccgc agcaggcacc ctgaagatcg ccggcgagac ggtcaacgac
     7561 atcgcctcga gtgtctggag cactcacggt gtggtgtgcg cggccagcaa catggccgtc
     7621 gacgcggttg agactgcacg agatggcgca cggacgaaac tgtggcagat gtcgcatgac
     7681 ctttcggaaa ggctgcacac cgcttccgag aactacaaca atgcggactg gctcgcgggt
     7741 cgcgacattg actcttgcag actgtgaaaa cggctcgcca cgttgaggat acgcacgatg
     7801 accgatgacg cgacgcgtcg cgcggcctgg gaggacggcg acgacgatcg ggatggcggt
     7861 gcgtccgcgc tagacgcact cggtgagtac tccccagtct ccgaggaaga ggccgaccag
     7921 gtgctggccg ctctgttcac ggtcaccaat ccagcgagga cggtctcggc gacagccgcg
     7981 atcggcgggc gtctgcagca gattgcgctg tctgcgaacg cgaccgacat gacggaagcg
     8041 gaactcgcgg aagagatttg cgtgctcgca gatctggccc ggcagaaggc gcaggccgcg
     8101 cagcacgcgg tcatcgtcca gcagatgcgc tcgatgggac acgactccgt gatgaccagt
     8161 gggttcctcg aacgcgatat ggacctacca tcgcctgaga ccgtggaggc tcgtcgagcc
     8221 gaagtcttcg ccgcacgcta cccattcgac gacgaagcct gaccgcgacg gtatgcgccg
     8281 ctttctcata gccatcgcgg taatcagtgc ggtagtcgcc gtaacggcgg ccgtcgtgtg
     8341 gtggatacag gatgacggca gtgccgcggc gcgccgggct tggcagccac caccaagccc
     8401 ggtcttctcc tcaccgatgc gcgtgcagcc cgtggcaggc tggcggatga gcacgaccga
     8461 tctcgggctg ccgccgccgg tcgatggcat ggagccgggt cggattggta ctgacaacga
     8521 tcccttccgt tcccgtccgt tcatcggcaa cctcgacgac catgcgtatt tcttggcgag
     8581 tacccctgca acgcctggtc cgcaatggtg gatgatcggc gtcgacgtgc gcagcggcaa
     8641 gcgtctgttc gctcccattc aattgggcgc gacgacactt ccgcctgaat gcttcctcaa
     8701 tggccgcgca aacatgttgt gtatcaacga tgaatcgcat accgcctacg tggttgaact
     8761 ccggtccggc accgtgaccc acactggccc aaccgacctg cgcctgggat atgggaagct
     8821 cgccgtggag caagttggga tttatgccgt cgccaaaacc caggaccaag gcgtatacgg
     8881 cattggacca cgagccgaaa ccacatggtt cgtgccgggc gacggactta ttcggccgat
     8941 tacagaaacc ggattcgaga caacaccgca gacgctcggt gcccaactga gcacagacgc
     9001 tcgcgcctac gatatgaccg tgttctcggt gagcgacggc aaggtggtca agcccgagat
     9061 acaccacgac gcacatctgg gagaaacaga aatctacccg ggcggttttg ctgccgcgat
     9121 cgagaccaaa ccaaaaaggt tggaggtcca cttctttgat gacaccggca aacgactcag
     9181 cgaccgtgcc gtccacggcg tagtcaggga atcaatcggt ctaccggtgg tcgcgtcgga
     9241 cgatcagtcg accatctact ccccggcagg caagaagctc ttggaccttc cggagggggc
     9301 gacccgcctt gtcggatcca cactcttcgt caacgagaac gacagcgacg cctttccgat
     9361 gtggcgccag tacgacctgg agaccggaga caagggcccg gcgtgcgact tcagcatgtc
     9421 gaacttcttg ggcaccaatg gatcggtgat cgtcttcgag gtcaggaacc caaaggccga
     9481 aatcgtggcc aaggcacgtg atttggccac gtgtgacacg ttgtggaccc tgccaaagcg
     9541 cgtcgattcc ttcgccaagg tgtggcgtgt caacacgaca ctggtccagc tatccgacga
     9601 cggaacggag ttgttctcac tggtcgcgcc gagctaggac gctgtccact ccaacagctt
     9661 gtcgacggac caggtgttga cgatccgctc cacgggcacg gcgttctcca ctgcgcgctg
     9721 cgcgccgtag ccgaggaact ccaactggcc aggggcgtgt gagtcggtat cgatggagaa
     9781 tagacacccg atctccaacg ccagcttcag cagccgcgtc ggcgggtccc gacgttcggg
     9841 gcgagaattg atctcgacgg cggtgccgtg atcgcggcag gcggtgaaga ccttctcggc
     9901 gtcgaacttc gactcgggcc tgataccacg accaccggtg acgagacggc cggtgcagtg
     9961 cccgagcaca tcggcatgtg gattctgcac agccttgagc atccggcgcg tcatcgatgg
    10021 cgcgtccatc gaaagcttcg aatgcacgct tgccacgacc acgtcgaggc gcttgagcag
    10081 ttcttcctct tggtcgagcg aaccgtcctc gagaatgtcg acctcgatcc cggtcaggat
    10141 ccgcatcggc gcgaccgcgt cctgcagctc atcgatgacg tcgagctgct tgcgcaggcg
    10201 ttcgggcgac aggccgttgg caatcgtcag ccgaggcgaa tggtcggtca gagcgcagta
    10261 ctcgtggccc aattcccttg ccgcgagcat catctcgtcg atcggcgccg agccgtcgga
    10321 ccaattcgag tgcacgtgaa ggtcgccctt gagtgccgca cggatgtcgc cgccgccgag
    10381 atccttgaca ttggaccgca attcgacgag cgcatcgggt tcctggccgg cccaggcctg
    10441 cgcgatcacc ttggcggtct tgggtccgat gccgggcagc gactgccaac tgttggcggc
    10501 gccatggcgt tcccgctcgg cttctgtaag tccttcgacg atgtcggcgg cgttgcggta
    10561 ggccatcacc cgtcgagagt cctcgcgggc acggtccttg taataggcga tctggcgcag
    10621 agcgatgacc ggatccatgc gtacagtctg ccccgtcagg gcgagtgcca acctgggtcg
    10681 cgcccgctga gcgcgacgat ccggtcaagt accggcgcgt cgtcggcgac cgggaccgcg
    10741 gcgccgaaca aaccgtcgac gggaccctgc gccgcgaact cggcgacgtt ggcgagcacc
    10801 gcctcggcga tgtcgggttc tatgtcgtag ggctgcccgg tcgcacgggc aagatcccat
    10861 ccgtggatga cgacttcggt gagccccacc atcgcgacgg cctcgcccgg catgtccatc
    10921 gctgcgatcc gggtcattcc ttgccaagcg ctcggatccg tccacgccga agcgagttcg
    10981 gccagccgcg tcgggtagct cgcccgccaa tcgggatcca gctgggcagc gtcgacactg
    11041 ggaggcgtgc cggtcagcgg cccgcggtcc ttgcgcgcgg cggcggcaaa tgccggggcc
    11101 aggccaccga cgtggtgcaa gacgtggccg acggagtagt ccgcgcatgg cgtcgcggcg
    11161 gtgagcttgt cgtcggtgat gttcgccagc acggcggctg tgcgctgaca cgcgggggtg
    11221 aaatcgatca tgtctctact gaccggccgc gcgacggtaa ctcatcgctg cgtgcaacgg
    11281 cgccgcgcac gagcgctcga gtctggttct cggcggcgcc accggtgaag aacaacattt
    11341 cgtcgcccgc ctcgaacacg tcgtccggct tcggaacgat gggctcgccg tcacgaacca
    11401 tgatggccag tgcagtgttc tcgggcaatg cgaggtcgcc cacccgcgta ccgacaagcg
    11461 gattgtcttc cggcagcgtc aatttggcca tactcacttg gccctgccgc agttccatca
    11521 gttgcacgag gtggccgatg tcgatggcac cctcgatcga cgccatcatg gcgcgtggtg
    11581 tgcagaccgc aacatcgacg ccccatgcct ccgtgaacag ccattcgttg cgcatatcgt
    11641 tgactctggc gaccaccctg gccacgccga attcggtctt ggccagcaat gccgccgcga
    11701 ggttgacctt gtcgtcgcca gtcgcggcga tgacaacgtc gcacgtctgc attcccgacg
    11761 cttcgagcga ggacagctcg caggcgtcgg ccaacaacca gtcggcgtcg ggcacggtgt
    11821 ggggttcgta gtgaccgacg ttgtgctcga tcagcaagac cttgtggcca tagtcgagca
    11881 gttcctgcgc gacggatcga ccgaccgccc cggctccggc gatggcgatt cgcatcatct
    11941 gccgctcgtc agcgcggtcc gacggctcgt cggatcgctt tctgactctg aacacgaact
    12001 caatactgtc ccatccggcg tggtaggtgt taactgcacg ccctaccacc cacggccccg
    12061 aacggcgatg aaatgacctt gcagcagctg tatctggtgc tgctgggcgg tgggctggta
    12121 ctgctggcca gcatcgtcgc cacccgggcc gccagccgag tcgggttgcc cagcctgctg
    12181 ctgttccttg gcgtcggcgt catcgtcggc gaagacggac tggggctgca cttcgacaac
    12241 tacctccttg ccgatcacct cggcacggtg gcgctggcga tcatcctcgt cgaaggcggc
    12301 ctcacaacgc ggttcggcga tgtgcgaaag gtattggcac ccgcggcggt gttggcgacc
    12361 gtcggcatcg cggtcagcat ggcggtcaca gccgtcgggg cgcacctgct gctcgggatc
    12421 gactggcaac tggcactact gctcggcgcg gttgtctcct ccaccgatgc cgccgcggtc
    12481 ttctcggtgt tgcgcgtcgt gcctctgcca cgccgggtcg ccggccttct ggaggcggaa
    12541 tccggattca acgatgcccc cgccgtcatc ctcgtgctga tgttcagcgt ggtgccactg
    12601 gagttgtccc cgttgcacgc gctcgccgag ttggcctacg agttgagtgt cggcgccgca
    12661 atcgggttgg gttgcggatt tctcggcgca ctcgctctgc gccgcatcgc tcttccggcc
    12721 tccgggctct atcccctcgc gactttcggg ctgggcatgg tcgcgttcgc cgcggcaggc
    12781 agcgcccacg ccagcggttt cctcgccgcg tatctggccg cggtcgtgct cgccaattcc
    12841 ggactgcctc accgctcggc gacccggtcc ttcgcggaag ggctcggttg gcccgcacag
    12901 atcggcttgt tcgtgctcct cggcctgctc gtcgacccca gcgacctggg cgccgaattg
    12961 gttcccggga tcgtcatcgg gctggtcctg ctgttggtcg ccaggccggt gtccgtcctc
    13021 gtctccctgg ccggattccg cgtgccctgg cgcgaacagg tcttcctgtc ctgggccggc
    13081 ctgcggggcg ccgtgccgat cgtgttgacg acgtttccga tcgtcggggg cgtgcccgac
    13141 agcatgcgcc tgctcaacat cgtgttcatc ctggtggtgg tgtacaccct ggttcaggga
    13201 cccaccctgc acccgctcgc ccacaggttg ggcttgattc ccaaggaagc cacccgcgaa
    13261 atccaggtgg aagcggctcc gctggacgtc ctggacgccg aattgctgac gatgacggtg
    13321 cacgcaccgt cccggctgca caacgtgacg gtcctcgaac ttcggttgcc cgatcccagc
    13381 gtcatcacac tgatcatccg gaacggccac acgtttgtgc cccagcccga tacccgcatc
    13441 gcgatcggcg acgaactgct catcgtcacc accgtgaaga cgcgcgcggc cgccgagcgg
    13501 aggctccgcg cggtgagtcg ccgcggcaag ctcgcccatt ggttcgacga gtacggcgaa
    13561 gcagactgag gcagccgact ttcaggaagt gggactaggc tcaacgcagt gagattcgct
    13621 ttcaagacgt ccccgcaaaa caccacgtgg tccgacatgc tggccgtctg gcaagcggcc
    13681 gacgacatcg atgtcttcga gtcgggctgg accttcgacc atttttatcc gatcttttcc
    13741 gactcgtccg gaccctgcct cgagggctgg accacgctga ccgctctggc gcaggcaacc
    13801 aagcggctgc ggctcggcac gctcgtcacc ggcatccact accgccatcc cgcggtgctc
    13861 gccaacatgg ccgcggcgct ggacatcatc tccgacggcc ggctcgagct gggcatcggc
    13921 gcgggctgga acgaggagga atccggcgcg tatgggatcg agctcggcag catcaaagag
    13981 cgcttcgacc gcttcgagga agcttgcgag gtgctcacca gcctgctgag caaggacacg
    14041 acgacgttcg acggcgggtt ctatcagctg aaggatgcgc gcaacgagcc gaagggcccg
    14101 cagaaaccgc atcctccgat ctgcatcggc ggcagcggcg agaagcgcac gctgcgcatc
    14161 accgccaagt acgcccagca ctggaacttc gtgggcgggc cgccggagga gttcgcccgc
    14221 aagcgcgacg tgctcgcctc gcactgcgcg gacatcggcc gtgatccgaa ggaaatcatg
    14281 ctgtcggcgc atgtccggct gagtcccgac caccacaacc cagacagccc gaccgatttc
    14341 agccacgcga tcgatgaggc cgcagcgctt ggcaaggagg ggctggatct cgcgatcgtg
    14401 tacctgccga cgccccacac tccgtcggtg ctggaaccgc tggccgaggc aatcagggac
    14461 tcaggcctgc tcaccagcaa agacaatgga cgataacgaa aatataacga ttaggcaaac
    14521 acaacatcac accgagtccg gtcgaagtaa ccgacgggtg cgggttcgct agacgccgta
    14581 acgaagtagc tcgtcgggcg tgatcagccg ctcggccttg gccgggaatt cgcggcactt
    14641 cattgggtga cgtaaccatg accaggcgat tcgactcacc cgagaccgag atagcgggtt
    14701 gaggtacacg gtgctcactc ctgcgatacg gcaatagctg aaccgggggc ttacgttacc
    14761 gcaaaaccct catcaagtca ttagataccc actatccggc aaactgaacg aggcgcgccg
    14821 agcaatcatc tgatgttttt ggagtgacta atgtgactaa tgaggtgtcg cacaggtacg
    14881 gcgtcaggtc tggcgttcgg gtttgacgaa gacatcgtcg agcgtgaccg tctggagatt
    14941 ccgtgagcgg atgacgtcga tcagttggcc gtagacgtgc gtgaccggca ggtggttgag
    15001 atggccgatc acgatcgccc gcgggttgaa gtacttgttg gccatcttga cgatctcgtc
    15061 ttctccgatg acgttctggt cctccaacga accggaccac atcgtgggca tcgtgtagcc
    15121 gagttcggcc gcgacggcgt cgacgacggg gttgtggctg ccgtacggcg gccggaagta
    15181 cggccgcgca tcgacgccgt agtttctcat caggaactgg tggttgcgcc gaagttggtc
    15241 cgccacttgg tctttcggca atattgccaa gtccgggtgt gaccacgtgt ggttgccgag
    15301 ctgaatctga cccgactcca ccaacggcca cagcagatta cggttttcgg tccacgaccg
    15361 gtaggtgccg ttgacgaagt acgtcagccg gacaccggtg tccttcgcga actccgtgta
    15421 gagccgcacg acatcgctgc tcacgccgtc gtccagcgtc agcgccagca ggttgccgcc
    15481 gcccggaatg ctgctcaacg cgccgccgcc aggcagcggc atccgcaccg cgaccaccgg
    15541 cggcgcaatg ggcgccgctg ccgcctgtgc aagccgcggc tgcgccccga cggtgcaccg
    15601 cgccaggccg acaccggcca gtgttgccag cgataggccc gcaaggaagt gtcgccggtt
    15661 tagctcagtc ccggcgtagg tgatcggtcg gcgcccagtc atcgggacta ctgtctcgcg
    15721 acagcaagca aaagccgctg attcggctgc gtgtcgcagc cgtttgctat ttaacgcgcg
    15781 ggttagcgct gcggaacggc ggtcggcttg atcggtgctg gcagcgccgt ttggcccatc
    15841 aggtagcggt ccacaccggc cgcggcggcg cggccctcgg cgattgccca gacgatcagc
    15901 gactgcccac gacccatgtc gccggcgaca tacacgccgg gcaccgtggt ggcgaaattc
    15961 gcatcgcgcg cgacgttgcc acggtcggtg atctcgacgc cgagatcggt cagtaggcct
    16021 tcacgctccg gaccgacgaa gcccatcgcc agcagcacca aatcggcttc catctcgaag
    16081 tcgctgcctt cgaccttctc gaacttgccc gcgttcatca cgacctcgtg cccgcgcagc
    16141 ccggtgacgt gcccgtcgct gccgctgaac tcctcggtgt tgacggagta cacccgctcg
    16201 ccgccctcct cgtgcgccga ggacacccgg aacatcagcg ggtagaccgg ccacggggtg
    16261 gaatccgcac gcgtctccgg tgggcgcggc atgatctcga actggtgcac actcgcggcg
    16321 ccctgccggt gcacggtgcc caggcagtcg gcaccggtgt caccgccacc gatgatgatg
    16381 accttcttgc cgttcgcgtg gatcggcggt aggccgttgt cgtcggtcac cgggtcgccg
    16441 tgctggacgc ggttggccca cggcaggaac tccatcgcct gatggatacc gtcgagctcg
    16501 cggcctggag caggcagatc acgccatgcg gtcgcgcctc ctgccagcac caccgcgtcg
    16561 aagtcggccc gcagctgcgc ggcggtgatg tcgacaccga cgttcacccc ggcgcggaag
    16621 acagtgccct cggcctccat ctgcgtcaga cgacggtcga tgtggcgctt ctccatcttg
    16681 aattcgggga tgccgtagcg cagcaggccg ccgattcggt cgtcgcgctc gaacaccgtc
    16741 acggtgtggc cggcacgcgt cagctgctgg gcggccgcca gacctgcggg cccggatccg
    16801 acgacggcga cggtcttacc ggtccgcttg tcgggtggca gcgggacgac ccagccgttg
    16861 tcgaaggcgt tgtcgatgat ttcgacctcg acctgcttga tggtgaccgg gtcctggttg
    16921 atgccgagca cgcacgagcc ctcacacggc gccgggcaca gccggccggt gaactccggg
    16981 aagttgttgg tggcgtgcag ccgctcgatc gcatcgcgcc aacgatccga gcgcaccagg
    17041 tcgttccact cgggaatgag attgccgagt ggacagccgt tgtggcagaa cggaataccg
    17101 cagtccatgc accgcgccgc ctgctcgcgc agcgtgtcgt gggagaagtc ctcgtagacc
    17161 tctttccagt ccatcagccg cagcgggacc ggccgccgct gcggggtctc gcgctgtgtg
    17221 tacttgagga agcctttggg atcagccatt cgccgccgcc atgatcgcct cgtcgacatc
    17281 tgagccggtg cgttcggcct ctgctatcgc cacgagcacc cgcttgaaat cgcgcggcat
    17341 caccttcacg aagttcttca gctcgttctc ccagtcggac aggactcgct gtcccacagc
    17401 cgaatccgtc gcatcgacat gcgcctggat cattccgcgc aaccactcga cgtcgtcatc
    17461 gtccatctcg tccaggtcga ccatctccgc gttgaggttg tcgcccagag ctccagcggg
    17521 gtcgtagacg taggccacgc cgcccgacat gcctgccgcg aagttgcggc cggtgggccc
    17581 gaggatgacg accttgccgc cggtcatgta ctcgcagccg tggtcgccga caccttcgac
    17641 gaccgcatgc gcgccggagt tacggaccgc gaaccgctcg cccaccacgc cgcgcaggaa
    17701 cacctggccg ctggtggcac cgaacaggat cacattgccg gcaatgatgt tctgctcggc
    17761 gacgtagtcg gcgggcgcgt tgtccgacgg gcgcaccacg atgcggccgc ccgacaggcc
    17821 cttgccgaca tagtcgttgg cgtcgccgta gacgcgcagc gtgatgccct tggggacgaa
    17881 cgcgccgaag ctgttgcccg ctgagccatc gaacgtgatg tcgattgttc cgtcgggaag
    17941 cccctgcccg ccataggctt tcgtcagctc gtggccaagc atggtgccca cggtgcggtt
    18001 ggtgttggcg atcgtggtgg agaacctcac cggtgtgccc gagtcgaggg cctcgcggct
    18061 catcacgatc agctgctgat ccagcgcctt gtccagcccg tggtcctgtc gcgagctgca
    18121 gtacagatcc tggttcatga aggccgactc cggctcgtac agcacgggcg ccaggtccag
    18181 cttgtaggcc ttccagtgct cggccgcacg cgtggtgtcc aacgacccta cttggccgac
    18241 catctcgttg acggtccgga aacccaactg ggccatcatt tcccggactt cctcggcgat
    18301 gaacatgaag aagttctcga cgaactccgg cttgccgttg aatcgcgccc gcagtgtcgg
    18361 gttctgggtg gccacgccca ccgggcaggt gtcgaggtgg catacccgca tcatgatgca
    18421 gcccgacacc accagcggcg cggtcgcgaa accgaactcc tcggcgccga gcagcgcggc
    18481 gatcaccacg tcgcggcccg tcttcagctg gccgtcgacc tgcacgacga tccggtcgcg
    18541 tagcccgttg agcagcagag tctgctgggt ctcggccaag ccgagctccc acggcgcgcc
    18601 cgcgtgcttc tgcgacgtca aaggcgtcgc gccggtgccg ccgtcatggc cggagatcag
    18661 caccacgtcg gcgtgcgcct tcgacacacc cgcggcgacg gtgccgacgc cgttctcgct
    18721 gaccagcttc acgtgcacgc gggcctgcgg gttggcgttc ttgaggtcgt ggatcagctg
    18781 cgccaaatcc tcgatcgagt agatgtcgtg atgcggcggc ggcgagatca ggccaacgcc
    18841 cggcgtcgag tgccgtacct cggccaccca cgggtacacc ttgttacccg gaagctggcc
    18901 gccttcaccg ggtttcgcac cctgggccat cttgatctgg atatcggtgc agttggtcag
    18961 gtagtggctg gtcacgccga atcgcgccga cgccacctgc ttgatcgcgc tgcgccgcca
    19021 gtcgccgttc tcgtcacggt caaaccggct gacatgttcg ccgccctcac ccgaattcga
    19081 ccggccgcca aggcggttca tcgcgatcgc caacgtctcg tgcgcctcgg cggagatcga
    19141 gccgtagctc atcgcacccg tcgagaaccg cttgacgatc tcgctggccg gctccacctc
    19201 gtccaacggc accggcgcgc gtacgccgtc cttgagcttc agtagaccgc gcagcgaggc
    19261 catccgctca ctctggtcgt cgaccagctt ggtgtactcc ttgaagatgg agtactgccc
    19321 ggtgcgggtg gagtgctgca gcttgaacac cgtgtccggg ttgaacaggt ggtactcgcc
    19381 ctcacgccgc cattggtatt cgccgccgac ctcgagctcg cggtgcgccc actcgtcggg
    19441 ccggtcgagg tatgccagcg cgtggcgggc cgcgacgtcg tcggcgatgt cgtcgaggtc
    19501 gatgccgccc gtcgggcagg ccagcccggt gaagtactcg tcgagcatct gctggctgat
    19561 accgatggcc tggaacagct gcgcgccggt gtaggacgcc agcgtcgaga tgcccatctt
    19621 ggacatcacc ttcagcacgc ccttgcccgc cgccttgacg tagttggcct tggccttgtc
    19681 gctgctgata ccggtgatca cgccgcggtc gaccatgtcc tcgatggact cgaacgccat
    19741 gtaggggttg atcgcggccg cgccgaagcc gaccaggcag gccatatggt gcacctcgcg
    19801 ggcgtcaccg gcctccacga cgagcccgac cttggtgcgg gtgcggtcgc gaacgagatg
    19861 gtggtgcacg gccgagacgc tcagcagcga cgggatcggc gccatcgact cgtccgactc
    19921 gcggtcggac aacacaatga tgcgcgcgcc gtcgcggatg gcggccgaca ccttggcgcg
    19981 gacgttgtcg agggcctcct tcagcccctg cccgccgcgg ttcaccgggt acaggcagcg
    20041 gatcaccgcg gcgcgcatgc cgtgcttgtg gccgcggatc tcgtgatcgg gatcgacgca
    20101 gatcagtttc gacagctcgg cgttgcgcag gatcggctgt ggcagcacga tctggcggca
    20161 cgactccgcc gtcgggttga gcaggtcgcc ctccgggccg acggtgcccg acaggctggt
    20221 gaccacctct tcgcggatgg cgtccagcgg tgggttggtc acctgcgcga acaactgctg
    20281 gaaatagtcg tagagcatcc gtggccgttg cgagaggacg gcgatcgggg tgtcggtgcc
    20341 catcgacccc aacgcttcta caccgctgcg cgccatcggc gccacgagca gatttagctc
    20401 ctcgtaggtg tagccgaaga tctgctggcg caacaccact cggtgatgcg gcatgcgcac
    20461 gtagtcgcca ggcggcagct cgtcgaggtg gaagagaccc gcgtccagcc actcctgata
    20521 cgggagctcg gcggccagct cggccttgat ctcctcgtcg gcgacgatgc ggcccttgga
    20581 cgtgtcgacc aggaacatgc ggcccggctg cagccgcatc ttcttgacga cggtggacgg
    20641 gtcgaggtcg agcacgccgg cctcggacgc catcacgacc aggccgtcgt tggtgaccca
    20701 aatccgtgac gggcgaaggc cgttgcggtc cagcaccgcg ccgatcacgg taccgtcggt
    20761 gaacgtcatc gacgccggtc cgtcccacgg ctccatcagc gatgcgtggt actgatagaa
    20821 cgcccgccgc gcgggatcca tcgactcgtg gcgctcccac gcctcgggga tcatcatcag
    20881 caccgcgtgc ggcaggctcc gcccgccgag gtgcagcagt tcgagcacct cgtcgaaccg
    20941 cgcggtgtcc gatgcgcccg gcgtacacac cggcacgacc ttttcgagat cctcgccgcc
    21001 gaatacatcg gtgttgatca gggcctcgcg cgcccgcatc caattctcgt tgccggtaac
    21061 ggtgttgatc tcgccgttgt gggcgatgcg ccggaacggg tgggccagtg gccacgacgg
    21121 gaacgtgttc gtcgagaacc gcgagtgcac gatgcccagc gcgctggtga gccgatcgtc
    21181 ctgcaggtca aggtagaacg ccttgagctg aggggtggtc agcatgccct tgtagacgaa
    21241 cgtctgaccg gaaaggcttg ggaagtaaac ggtttcgcgg cctgggccgt cctggccggg
    21301 acccttggtg ccgagctcgt gttcggcacg cttgcgcacg acgtacgtgc ggcgctccag
    21361 atccatgccc gacgcgcccg ccatgaacac ctggcggaac gtcggcatcg cgtcgcgcgc
    21421 gagggcacca agagacgact cgtcggtggg cacctcgcgc cagccgagca cctcgaggcc
    21481 ctcggcctcg gcgatcttct cgaccgcgtc gcacgccgcg gcggcgtcct tggaggactg
    21541 tggcaggaag gccatgccgg tcgcgtagct gccgtactcg ggcaggtcga attcgacgac
    21601 ggcgcgcagg aactcgtcgg gaacctgcag cagaatccct gcaccgtcgc cggtgtgcgg
    21661 ttcagcaccc tgggcaccgc ggtgctccag gttcaccaga gccgtaatgg ccttgtcaac
    21721 gatgtcgcgg gtgcgacggc cgtgcatatc ggcgaccatc gccacgccgc acgcatcgtg
    21781 ctcgaacgcg gggttgtaca gcccttgaat acggggcgtc attcccacct gacccttcgc
    21841 gaagctttca tggcagccgt cgacgacggc attagccggc ttggcgatgg aggtgccttc
    21901 gtgcagcact gaacgacggc ccaatcccac tcgccacaag tggagaaaac gatatgacaa
    21961 acaccgcctg acatgccaac ttagtcaaac ctaactatac tcgcggcgcg gaggcacagc
    22021 ggtggcgatc agggcatcga taacgaacga ataacgttgc cgctgttggc ctttcgaggc
    22081 gggagcggta ctgcgggtgt cgtgaggcac ggatcacatt ctgggaccat tcatccacaa
    22141 tgtttgctgc gatggacagc caagtctgta ggctgcggcg acaagatcac aatggtttgc
    22201 gtaaagccta gtcagattct tagagaagcg cctgcacact ggcctcgggc cgcaaatcca
    22261 ggcgccgcag cagctgggcg ttcagggcaa ccacgactgt cgagatcgac atcaagatcg
    22321 cgccgaccga catcggcatc acaaagccga tcggtgccag caccccggcc gccaggggaa
    22381 ccgagatcag gttgtatccg gctgcccacc aaaggttttg cttcatcttg cgatacgacg
    22441 ccttcgacag ctggatcacc gacagcacgg agcgcgggtc cgagctggcc aggatcaccc
    22501 cggccgaggc aatcgcgacg tcggtgccgg cgccgatcgc gataccgacg tcggcctggg
    22561 ccagcgcggg cgcgtcgttg acgccgtcgc ccaccatcgc caccttgagg ccttcgtcct
    22621 gcagctgcgc caccttggcg gccttgtcct cgggccgcac cccggcgaac acccggtcga
    22681 tgcccagctc ggcggccacc gattccgcga cggcccgtgc gtcacccgtg atcatcacca
    22741 cctggatgcc gagcttgtgc agcgcgtcga cggcctgccg ggattcctcg cggatctcgt
    22801 ccgccagcgc cagcgcgcca acgacggtcc cgttgacaag cacgtgcagg atgatcgcgc
    22861 cttgttcgcg ccacgggcgg gcctcgggca gctcattgcg gcccgcctcc tcgagcagac
    22921 gcggcccgcc gactcgcacg atggtgccgt cgactcgcgc ggtcacaccg acggcaggcg
    22981 acgaggtgaa gtccgacgac ccggggaccg tcaggccgcg gcgccgggcg gcgtccacga
    23041 tggccttcgc gagcgggtgc tcggagtcgg actcggcggc cgccgcgatc gcaagcacgt
    23101 cgtcctcggt gcggccgtct gtggtcgcca cagccgtcac cgtgggctcg cccttggtca
    23161 gcgtgccggt cttgtcgaac agcaccgcgc cgaccgtgcg catgctctcc agcgccagcc
    23221 ggtccttgat cagcacgcca cctcgagccg cgcgttcggt cgcgatcgag accaccagcg
    23281 ggatcgccag gccgagcgcg tgcgggcacg cgatcaccag cacggtgatg gtgcggatga
    23341 ccgcctcctc gggttggccg atgaagcccc acaccaccgc ggtcacgatc gccgcgccaa
    23401 gagcgaacca gaacagccag gccgccgccc tgtcggcgag ccgctgcgcc cgcgacgagg
    23461 agttctgcgc ctcggcgacc agccgctgga tgccggccag cgcggtgtcg tcgccgacgg
    23521 cggtgacctt gacgcgcaac ccggagtcgg tggcgaccgt gcccgcgacc accgcgtcgc
    23581 cttggctgcg gcgcacgggc cgggactcgc cggtgaccat cgattcgtct atgtcggcgg
    23641 cgccgtcgag gatgacaccg tcggcgggca cgctgccgcc gggccgcacg atcacgacgt
    23701 cgtcgacccg gagctccgac ggtgcgacgg tctcggtgcc attgggggtc acgcgctccg
    23761 cttcatcggg cagcagcgcc gccagcgagt ccagcgccga cgtggtctgc gccagcgatc
    23821 gcatctcgat ccagtggccc agcagcatga tcacgatcag cagcgcgagt tcccaccaga
    23881 agtcgaggtt gtgcggcagc acgccgacgc tggcgcccca ggacgatacg aacgcgacgg
    23941 tgatggccag gccgatcagc agcatcattc ccggtgcgcg ggaacggatt tcgctgatag
    24001 cgccggtgag gaacggccgg ccgccccaca cgtacatcac ggtgcccagc agcggcgaaa
    24061 cccattgcga tccggggaat accggcacgg agtagccgag gatcatcgcg aacatgcccg
    24121 acagcaagac cgtcgggacg gcaagcacga gcatggtcca gaacagcctg cggaactgcg
    24181 cgacgtgatc tccgtggccc gcgtgagcgt gctctgcgtg gtcggcgtgc acctggtgct
    24241 gcgtgtgggc gacctcggtc atgcccgcca ctttataccc cctaggggta tatggctagg
    24301 cccgcgtcat ggcctacctc ggataacatc cgcctcatgt ccgaggcccg tcgagccgct
    24361 atccgaggaa ggcgatgacg gccccggcgg caggtcccga tcagcccgac gattccatgc
    24421 ggggttcggt acacatctgc gtgacggcga tcgtggcggg cgtgttgatc ggcttcgttg
    24481 gcggcgcgtt ccgctggtgt ctgcagaaag ccgatgagtt gcggatcgac ctggtcgact
    24541 gggcgcacgg gctgccgggg ccgggctggc tggtccccat ggccgccgcc gcggccggcg
    24601 ccacccttgc cgccctgatc gtgcggtggg agccgctggc cgccggaagc ggtatccagc
    24661 atgtcgaggc ggtttttctg ggtgaggccc gaccaccgct gatccgattg gttccggcga
    24721 agttcatcgg tggtgtgttg agcatgggat cggggctggt gttgggtcgc gaggggccga
    24781 cggtgcacat gggtgcggcg atcggagccg aagcggcgcg ccgcgcccgg ctacccgatt
    24841 ccgaaatcag gatgatgcag acggcgttgg gcggcgccgg cctggcggtc gcgttcaacg
    24901 caccgatcgg cggcaccctt ttcactctcg aggaggtcac gaagtccttc cgggtgaaaa
    24961 cggttctggc aacgctcttt tcggctgctg cagctgtggc gtgctcccgg ctgatcctgg
    25021 gaaaccatgc ggacttccag gtcgaaccca tcgatgctcc ggccctggca tggttgcccc
    25081 ttttcgtcgt cttcgggctc ctgaccggat gtttgggtgc cgcctacaac tggttggtcc
    25141 tgtggttcct tgaccacgtc ggagcgatac gccgaatccc ggccctggcg aaggcagcca
    25201 taatcggtgc ggtcatcggg ttcgcgatgt tcgtgtaccc gcttgcggtg ggcggcggtg
    25261 agggcctgac acagcggatc ctcggcggag gtcacatcgt tcttgctgtc gtcatcggaa
    25321 tccttgcggt gcgattcatc gccggccccc tgtcgtactc ggcggcggtg ccgggcggac
    25381 tgttcgcgcc actgctggcc gtgggcgcgc tgtggggact gctgttcgcc ggcggtttca
    25441 acgccgcatg gcccgaaaac gcaacgcagt tggccgttcc gatggcgctc gtcggaatgg
    25501 cggccttctt cggggccact gtgcgcgcgc cggtgaccgg gatggtcgtc gtcaccgaga
    25561 tgacggccac gaccgagacg ctcgtgccga tgatggccgc gaccgctgct gccgtgttcg
    25621 ccgcgtacgt cgtgggatcg ccgccgatct acgagagtct tcgagagcgg atgcccgacg
    25681 aaacgtcaat cgaagaacgc ggtcgcggtt agcgttgcgc catgccaact acacacgcca
    25741 cgtcactgac aaacggcgag atcgtcgagg agaatgacct cggctcgatc cgacgagtga
    25801 ccgcggacag cctgccgatc ctgtcgggca tgtccatcaa gcggatcgtc gtcaatcccg
    25861 gcgcgatgcg cacacctcat tggcacgcca actgcaacga gctcacctac tgcgtgtcgg
    25921 gcacctcgtt cgtgtcggtg ctcgacagct acagcaagtt ctccagcttc acgatcagcg
    25981 ccggcgagat gttccacatc gactcgggct cactgcatca catcgaaaac atcggcgagg
    26041 agcctgccga gttcatcctc gcgttccgca gtgaacgccc cgaggacttc ggccttgcgg
    26101 ggtcgttcgg tgcgatgacg gacgcagtgc tgggcaacac ctacgacctg ccggcaagtg
    26161 atttcgccaa gatgcgacga gacaccaacg accgcaggct cgccgcccgc aaggcggatg
    26221 cggtcgtccc gtccaccgcg tatttcgatg acccgcacaa gttcggggtg gaggcgcaga
    26281 acccggtgat cgggatcgcc gtcggatcgg cgcggctggc gcgcgcccag tactggccgg
    26341 cgctgaagga cctgtcgatg tactccctgc ggatccggga agacggtatg cgggaaccgc
    26401 attggcatcc ggtcaccgcc gagatgggtt atgtccacaa gggttccggg cggatgacgg
    26461 tgatggatcc cgacggctcc ctggacacct actacctcaa ggagggcgac gtctacttca
    26521 tcccgcgcgc ctacccgcat cacatcgagg tcgtcgacgc gcccgacatt catttcgcga
    26581 tctttttcga ccagcccatg ccgggcgaca tcggttaccg cgcgtcggcc agcgcctact
    26641 cccgtgaagt gcttgccgcg acgttcaaca tgcacatcga cgaccttccg aacttcccgt
    26701 tcaccaagac cgacccgctg atcgtcaccc gcaacaaccc cgtcgacgaa cacgccatgg
    26761 ccgaacggta ggtcacgctg ccgtcgcgac aatttccatc gcggcacgat tcggcaacgg
    26821 cgaatccgca acggcctgct ccatgggact ctcaccgcac aggcgtacat ccaacggagg
    26881 agtcacgcat gcgcgaaccg acaatgctgc accgactggc agcggaattc atcggcacat
    26941 tctggttggt gttcggcggc tgcggcagtg ctgtgttcgc cgcgaagttc acctccgatg
    27001 ggacgtccct cggaatcggt ttcctcggcg tcgcactcgc gttcgggctg actgtgctta
    27061 ccggcgtgta cgcgttcggc acgatctcgg gtggccactt caatcccgca gtcacgctgg
    27121 gcgcagcgct ggctcgccgg gtggagtgga gggtgttgcc cgcctactgg atcacgcagg
    27181 tcgtcgccgg actcggcgcg ggcatgatga tctacgtcgt cgccaagggc caggagggtt
    27241 ggaccgcgac aggtcatatg gcggccaacg gttttggcgc ccactctccc ggcggatatt
    27301 cgctgtgggc ggtgatcgtc gccgaggtcg ttctcaccgg ggtgttcctg ttggtgatcc
    27361 tcggctcaac cgatgaccgc gcaccaaaag ggttcgcggg cttgtcgatt ggccttacgc
    27421 taaccctgat ccacctgatc tcgatcccga tctcgaacac gtcggtcaac ccggcgcggt
    27481 ccaccggggt cgcgttcttc aacggcgacg gcgcgcctgc gcagctgtgg gtgttctggt
    27541 tggcaccgct actgggcgca gccatcgcgg gcatcgccta cccatacctc tttggccgca
    27601 acgaggaact cgccgaccgc ccggtgcgtg acaacgctct ggaggaaccc gctactactt
    27661 gaccggcgcc ttcttcttgg ctttgctggc ggcggcccgc tccgtcttgg cacccgcgct
    27721 ggccaactgg ccgccggcgt tctcgagat
//
LOCUS       DASRJA010000582         3940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3960219,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000582 DASRJA010000000
VERSION     DASRJA010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3960219"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VFC01_25795"
     CDS             <1..880
                     /locus_tag="VFC01_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN83063.1"
                     /translation="QAKNDDLFYLPPEAFQNPDFKRGLDMFLSPDGKSARMFITHESD
                     PATVDGIKRVDGERRAAQEALKMSSLSNAKIYLGGVAATYKDMSDGARYDLLIAVVSS
                     LTLIFMIMLILTRSAVAAAVIVGTAASSIAASFGISVLIWQDLFGIQVQWLVMLMSVI
                     ILLAVGSDYNLLLVSRFKDEIHAGLKTGIIRSMAGTGGVVTSAGLVFAATMAAMMGSK
                     LIVLAQMGSTIAIGLLVDTFVVRSFLMPSIATLLGRWFWWPQVVYPRGDYHFLPPQPR
                     RRTRDDADTETLPAVT"
     gene            886..1341
                     /locus_tag="VFC01_25800"
     CDS             886..1341
                     /locus_tag="VFC01_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016887407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN83064.1"
                     /translation="MTRVLVTGMSGVGKSSLLQELSARGYRTVDTDYCDYHESVEGEW
                     LWREDRIAAMLDDDAPGPLFVQGTTRNQVVFYPLFDHIVLLSAPADVLVQRLRTRTTN
                     PYGKDPAEVAETLGYLETVEPLLREAATLEVVTTIPVGQVADTVVAHCL"
     gene            complement(1255..1779)
                     /locus_tag="VFC01_25805"
     CDS             complement(1255..1779)
                     /locus_tag="VFC01_25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN83065.1"
                     /translation="MSGTSIRRATTADLAEIRRVVKDSYTKYIERIGRPPAPMTADYA
                     AALEHSRVWVLESGDAIVGALVTEAHADHLLLEMVAVAPGAQGSGYGRLLLDRADSDA
                     TELGLTEVRLYTNEAMTENLTFYPRHGYRETGRALQDGYRRVFFSKTVRNDGVGDLPD
                     GNRGDHLEGGGLPQ"
     gene            1804..2577
                     /locus_tag="VFC01_25810"
     CDS             1804..2577
                     /locus_tag="VFC01_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HZN83066.1"
                     /translation="MRSTPKCWLTDMDGVLVREEQALPGAAEFLQRLVDRERPFLVLT
                     NNSIFTPRDLSARLKRSGLTVPEESIWTSGLATATFLNDQLPGGSAYVIGEAGLTTPL
                     HQLGYTLTDAEPDYVVLGETRTYSFTAITKAVRLILGGARFIATNPDVSGPSTEGPLP
                     ATGSVAAMITKATGREPYFVGKPNPMMFRSALNRIEAHSESTMMVGDRMDTDVVAGIE
                     AGLDTVLVLTGSTTVADIERYPFRPGRVLDSIADCIELV"
     gene            2664..3236
                     /locus_tag="VFC01_25815"
     CDS             2664..3236
                     /locus_tag="VFC01_25815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83067.1"
                     /translation="MIQSAIAGAVRRLVDNTLSSGVRPWDVPELVRRAADLGEVSSAR
                     AESVDENVLVGSLLPGGQRIVLRGVDDEWRLVRFVDGDDVSLRPETTRRVELRGSAPD
                     CVLTALGLVKPGQVELEVMSEYLGEGETETRYRYQWTEGSTSILAEEVENEIFDGATP
                     YSTYVRGVIVDRDRGVLLTGRDDSALIIEG"
     gene            3241..>3940
                     /locus_tag="VFC01_25820"
     CDS             3241..>3940
                     /locus_tag="VFC01_25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HZN83068.1"
                     /translation="MADIELIGVPFDGYGRTGHQARASLALRNAGLRDAFDHHHVIDD
                     DDLVLPGPDPGRGASTSLINERALLAMTDGLNRKVGEVVSAGRFPFVYGGDCSTLLGI
                     VTGLRDHVGDVGLVFIDGHEDTMPLDVSEDGEAANTEIGLLLGLTGRLLSGPLATRLG
                     ALRREELAVLGPRDVAWRQQFNVGTLRDTGVWFRDWKETAAAPEQQAEAAVAHLNQGS
                     DRWWLHVDLDVLDPQ"
BASE COUNT          652 a         1270 c         1304 g          714 t
ORIGIN      
        1 ccaggccaag aacgacgacc tgttctacct gcccccggag gccttccaga accccgactt
       61 caaacgcggt ctcgacatgt tcctgtcgcc ggacggcaag tccgcccgca tgttcatcac
      121 ccacgagagc gatcccgcga cggttgatgg catcaagcgg gtggacgggg agagaagagc
      181 cgcgcaggag gcgttgaaga tgtcctcgct gtcgaacgcc aagatctacc tgggtggcgt
      241 cgcggcgacc tataaggaca tgtccgacgg tgccaggtac gacctgctga tcgcggtggt
      301 gtcgtcgctg acgctgatct tcatgatcat gctgatcctg acccgaagcg cggtcgcggc
      361 ggctgtcatc gtcggtacgg ccgccagctc gatcgctgcg tccttcggca tctcggtgct
      421 gatctggcag gacctcttcg gcattcaagt gcaatggctc gtcatgctga tgtccgtcat
      481 catcctgttg gcggtgggat cggactacaa cctgctgctg gtgtcccgat tcaaagacga
      541 gatccacgcg ggtctgaaga ccggcatcat tcgctcgatg gcgggtacag gcggcgtggt
      601 gacatcagcg ggtctggtgt tcgcggccac catggcagcg atgatgggca gcaagctgat
      661 cgtgctggcg cagatgggtt ccacgatcgc catcggcctg ttggtcgaca cgttcgtcgt
      721 ccgctccttc ctgatgccgt cgatcgcgac gctcttgggc cggtggttct ggtggccgca
      781 ggtggtctat ccccgcggcg actatcactt cctgccaccg caaccccgac gtcgcacacg
      841 cgacgacgca gacacggaaa cgctgcccgc cgtaacctga cccatgtgac gcgggtgttg
      901 gtcaccggga tgtccggtgt cggcaagtcg tcgttgctgc aggaactttc ggcgcgcgga
      961 tatcgcactg tcgacaccga ctactgcgac tatcacgaat ccgttgaggg tgagtggttg
     1021 tggcgagaag accgcattgc tgcaatgctc gatgacgacg ctcccggccc gttgttcgta
     1081 cagggcacga cgcgcaatca ggttgtgttt tatccgcttt tcgaccacat cgtgttgctc
     1141 agcgccccgg ccgatgtgct cgtccagcgg ttgcggacgc gtaccacgaa tccgtacggc
     1201 aaggatccgg cggaggtcgc cgagaccctc ggatatctgg agacggtcga accgctactg
     1261 cgggaggccg ccaccctcga ggtggtcacc acgattcccg tcgggcaggt cgccgacacc
     1321 gtcgttgcgc actgtctttg agaagaagac gcgccggtaa ccgtcctgca gcgcgcgtcc
     1381 ggtttcgcgg tagccgtgcc gggggtagaa cgtcaggttc tcggtcatcg cctcgttggt
     1441 gtacaaccgg acctcggtca gcccgagttc ggtcgcatca ctatcggcgc gatcaaggag
     1501 cagtcgtccg taaccgcttc cctgggcacc gggcgcaacg gcaaccatct ccagcaacaa
     1561 gtggtctgcg tgggcttccg tcacaagcgc tccgacaatg gcgtcgccgc tttccagcac
     1621 ccacacccgc gaatgctcga gtgccgcagc gtagtcagcg gtcatcgggg ccggcggccg
     1681 accgatgcgc tcgatgtatt tcgtgtatga atccttgacc acgcgtcgaa tctcggccag
     1741 gtcagctgtc gtggctcggc gtatcgacgt cccagacatg cgcgccagca tagggtggac
     1801 ctcgtgcgct caacaccgaa gtgctggctc accgatatgg acggtgtcct cgtccgcgaa
     1861 gaacaggccc ttcccggcgc cgccgagttc ctgcaacgcc tggtggaccg ggagcggccg
     1921 ttcctggtgc tgacaaacaa ctcgattttc acgccccgcg acctgtccgc ccggctgaaa
     1981 cggtccgggc tcaccgtgcc cgaggagtca atctggacct ccggcctggc caccgcgaca
     2041 ttcctcaacg atcagctgcc cggcgggtcg gcctacgtga tcggtgaggc cggcctgacc
     2101 actccgctgc atcaactcgg ctacacgctg accgatgccg aacccgacta cgtcgtactc
     2161 ggcgagaccc ggacgtattc attcaccgcg atcacgaagg cggtccggct gatcctcggc
     2221 ggcgcgcgat tcatcgcgac gaacccggat gtcagcggcc cgtcaacgga gggtccgctg
     2281 cccgccaccg gctcggtcgc cgccatgatc actaaggcca ccggccgcga accgtacttt
     2341 gtcggcaagc cgaacccgat gatgttccgc agcgcgctca accggatcga ggcgcactcg
     2401 gagagcacga tgatggtcgg cgaccggatg gacaccgacg tcgtcgccgg catcgaggca
     2461 ggcctggaca cagtcctggt gctcaccggc tcgaccaccg tcgctgacat cgagcgctat
     2521 ccattccgtc ccggtcgcgt gctggactcc atcgccgact gcatcgaact cgtttgattc
     2581 cggttgaatt cgcgccaggg gtgcatgagt cgctgcgcgc gatggaggac cgtggcacgc
     2641 cgctgccgcg atgtcatagg ggtgtgatcc aaagcgcgat cgcgggtgcg gtgcggcgcc
     2701 tcgtcgacaa caccctgtcg tcaggtgtgc ggccgtggga tgtcccggag ctggtgcgac
     2761 gggccgccga ccttggcgag gtgtcgtctg cgcgtgcgga gtccgtcgac gagaacgtgt
     2821 tggtcggctc cctgttgccc ggcggacagc ggatcgtgct ccgcggggtc gacgacgagt
     2881 ggcggctggt tcgctttgtc gacggtgacg acgtcagcct tcggcccgag accacgcgcc
     2941 gggtcgagct gcgcggttcg gcccccgatt gcgtgctgac cgcgttgggc ctggtcaaac
     3001 cgggccaagt cgaactggaa gtgatgtccg agtacctcgg tgaaggcgag acggagaccc
     3061 gctaccgcta tcagtggacc gagggcagca catcgatcct ggccgaagag gtcgagaacg
     3121 agattttcga cggcgccacg ccctattcga cctatgttcg gggggtgatc gtcgaccgtg
     3181 accgcggcgt gttgctgacc ggcagggacg acagcgccct gattatcgag ggctgaacag
     3241 atggccgaca tcgagctgat cggggttccg ttcgacggct atgggcggac cggacatcaa
     3301 gcgcgggcat ccttggcgct gcgcaacgcc gggctgcgcg acgccttcga ccatcaccat
     3361 gtgatcgacg atgacgacct cgtactgccc ggcccggatc ccggccgtgg cgcgtcgacg
     3421 agcttgatca acgaacgtgc gctgctggcc atgaccgacg ggctcaaccg caaggtcggg
     3481 gaagtggtga gcgcgggccg cttcccgttc gtctacggcg gcgactgctc gacactgctg
     3541 ggcatcgtga ccggcctgcg cgatcacgtc ggcgatgtcg ggttggtgtt catcgacggc
     3601 cacgaggaca ccatgccgct ggacgtgtcc gaggacggcg aggcggccaa caccgagatc
     3661 gggctgctcc tcggcttgac cgggcgcctg ctctcgggtc ccctcgctac gcgactgggt
     3721 gccctgcgac gagaggagct cgccgtgctc gggccacgcg acgtcgcctg gcgccagcag
     3781 ttcaacgtgg gcacgcttcg ggacacgggt gtctggttcc gggactggaa ggagacggca
     3841 gcggcgccgg aacagcaggc cgaggcggcg gtggcccacc tgaaccaggg gtcggaccgg
     3901 tggtggctgc acgtcgacct cgacgtgctg gatccccagg
//
LOCUS       DASRJA010000583        26405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3517696,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000583 DASRJA010000000
VERSION     DASRJA010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 26405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26405
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3517696"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2321)
                     /locus_tag="VFC01_25825"
     CDS             complement(<1..2321)
                     /locus_tag="VFC01_25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HZN83069.1"
                     /translation="MFVADDRVVYSASDLAAAARCEYALLRSFDARLGWGPPVAVEDE
                     LLARTAKLGGEHEQRHLDELRADDTVAIIGRPPYTVAGLTAAAEQTMRAVERRAPVIY
                     QAAMFDGRFVGFADFLLFDGEQYRLRDTKLARSVKVEALLQLAAYADTLTAAGVSVSP
                     EVELVLGDGATATYRVDELLPVYLPRRAALQRLLDDHLASGAPVTWENDEVRACFRCP
                     ECALQVRATDDLLLVAGMRVSQRARLIEAGITTLSALTRHDGPVPELPARTVTSLTKQ
                     ARLQIAPRVDGKPPYEIADPQPLNLLPDPDKGDLFFDFEGDPLWTADGHEWGLEYMWG
                     VLDIGGGFKPLWAHDRASERQALVDFLTMVRKRRKRYPKMHIYHYASYEKTALLRLAG
                     RYGVGEDEVDDLLRSGVLVDLFPLVRKSIRVGTENYSLKSLEPLYMGSELRTGDVTTA
                     TESITMYARYCELRADGRNDEAANVLKEIEDYNRYDCRSTHRLRDWLIKIAIESSVPP
                     LGPQPVGDGTEVEDTDELARTFMTFAGDGVAERSPEQTAVALFAAARGYHRREDKPFW
                     WAHFDRLNNPVDEWGDTSDVFVADKAEVVQDWHVPSSRARKQQRWVKLTGTLAAGGLD
                     TNVFALYDPPSPAGLTENTDRRAAGNATITGVDDPNVPTEVTICEREPKDGGTFAELP
                     FALTPGRPFPTGKLRESIDTAAASVAAGLPALPDTAIVDILRRRTPRTRSGASLPHSG
                     DTTTDITAALLDLDSSYLAVHGPPGTGKTHTAA"
     gene            2514..3425
                     /locus_tag="VFC01_25830"
     CDS             2514..3425
                     /locus_tag="VFC01_25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HZN83070.1"
                     /translation="MNEASLPFIGTEAVGSGKVTRRTLASGHNMIYRNIYLPDGVELT
                     PDRRAVAAWLWSGRNATIAGMAAAALHRSQWIDLELPAELIRNEACKVDGIVIHRGRL
                     RDDEICVVQGMPVTTPARTAFDLARRDRLETAIIRVDALANATGLKPQDVERLAESRR
                     GARGIVQLRRVVELMDGGAESPQETRTRLLLIAAGFPKPQTQIVVVDEYGGFVGRVDM
                     GWAEWKVGVEYDGPQHWEDPDQHARDIDRLADLAAQGWLIIRVSRDLLRYRPHVFLAR
                     VRDAMRAAGWPHWDKIRLDARISWLAY"
     gene            complement(3495..4052)
                     /locus_tag="VFC01_25835"
     CDS             complement(3495..4052)
                     /locus_tag="VFC01_25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppP/LprE family lipoprotein"
                     /protein_id="HZN83071.1"
                     /translation="MKWAVLVVAATIAVAGCGSSDSTASKTPEPTVPSVAPATAPLSL
                     PPPPPIVAPTTSAAADPCAVNLAAPEIIKAVSELPRDPRSNQAWNPEPLAGNYTECAQ
                     LSAVIVKANTNSDNPNTRAVLFHLGKFIPTGVPDTYGFNGIDESATTGDTIALRYSGG
                     IEGLDSVVKFRWNGNGVELIGNTLG"
     gene            4140..5828
                     /locus_tag="VFC01_25840"
     CDS             4140..5828
                     /locus_tag="VFC01_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZN83072.1"
                     /translation="MTSPEPDATSADLTFADLQIHPSVLQAVGDVGYESPSAIQAATI
                     PAMLAGSDVVGLAQTGTGKTAAFAIPILSKIDPTNRTTQALVLAPTRELALQVAEAFG
                     RYGSHLPQINVLPIYGGSSYGPQLAGLRRGAQVVVGTPGRVIDHLEKGTLDLTHLDYL
                     VLDEADEMLQMGFAEDVERILADTPEYKQVALFSATMPPAIRKLTTKYLHDPVEVTVK
                     AKTATAENISQRYIQVAGPRKMDALTRVLEVEQGDGMIVFVRTKQATEEVAERLKARG
                     FSAAAINGDIPQAQRERTIAALKDGSIDILIATDVAARGLDVERISHVLNYDIPHDTE
                     SYVHRIGRTGRAGRSGSALLFVTPRERHLLKAIEKATRSKLIEAELPSVEDVNAQRVA
                     KFRDSITDALNAPGVDLFRSLIEDYERENDVPMADIAAALAAQARDGKQFFMVEPPPE
                     KRRRDERPDKHDRDRPKRKPGQGFATYRISVGKRHKVSPGAIVGAIANEGGLHRSDFG
                     HISIRPDHSLVELPVELPRETVKALERTRIQGILINLQPDRPPRKAGKNSRKSK"
     gene            5825..6964
                     /locus_tag="VFC01_25845"
     CDS             5825..6964
                     /locus_tag="VFC01_25845"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZN83073.1"
                     /translation="MTLSKDLDAQGGLESVSKPERVASLTGIRAVAALLVMFTHAAYT
                     TGKYTHGYVGLVYSRMEIGVPIFFVLSGFLLFGPWVRAAASDGEPPSVRRYAWHRVRR
                     IMPAYTVTVLVAYAVYHFRTAGPNPGHTWLGLFRNLTLSQIYTDNYLYSYLHQGLTQM
                     WSLAVEVAFYVMLPLLAYLLLVVLCRRRWRPGLLVAGLSGLALITPAWLILVHTTDFL
                     PDGARLWLPTYLSWFIGGMILAVLQPMGVRAYAFVCVPLAVVCYFIVSTPIAGAPTTS
                     PAELREALFKAAFYAVIATLAVAPLALGDSGLYARFLASRPMVFLGEISYEIFLIHLV
                     TMELVMVEILHWHIYTGNVAILFLVTLVATVPVAWLLHRVTRVRP"
     gene            complement(6965..7609)
                     /locus_tag="VFC01_25850"
     CDS             complement(6965..7609)
                     /locus_tag="VFC01_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HZN83074.1"
                     /translation="MTHNSYSTRIRRVAMATAALAALTVVTAAPAWAHVHVEADHAQP
                     GSEAILTFNVPNESEKGAATTELSVSLPNLTSVSTQQMPGWTVRLDRDAAAGTVKTVT
                     WTAASGAGVPPDQFALFRVEVLLPKADSVSFPATQTYSDGTVVKWDQPTPASGTEPEY
                     PVPTLALSGEPADEHHAMTAESSPPADSTARWLAAGGIAIGVVAVALAVMRRRA"
     gene            complement(7793..8326)
                     /locus_tag="VFC01_25855"
     CDS             complement(7793..8326)
                     /locus_tag="VFC01_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83075.1"
                     /translation="MSRPTYPAFLRGGLVGLCAALVTAAAHAGAGGGVPTGSALILLL
                     VVCATVGAAVGGIKLEGRHAHAFVVIGALGVGQALGHVTLSVGGGHHAGWLLTTPMLM
                     LHVLAALGLGLLISAVEYLYAVCASVLCWLRIFAAGWLRPEGRSWRPSNVVVARPVLP
                     RAGSGMRAPPQVSVVSA"
     gene            8421..9137
                     /locus_tag="VFC01_25860"
     CDS             8421..9137
                     /locus_tag="VFC01_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HZN83076.1"
                     /translation="MSDRMPSRGFSGAVLKLLRAGDYQLTVTGRREISPHYLRLSFDA
                     GGILTDRPVHPTMWIRMWFADGDKLHQRGYTLVDPDPAADTVDIEFALHDRVASRWAQ
                     DARPGDTIEVTVLGSNFTLPEPAPKGYVIVGDTASLPAVNSLLTAIGNTPAKVFLEAG
                     HDDDKQLPVARSADVVWVDRKNGGEALVRAVGSSAFDAADHFGWVACDNRTTRAVAKV
                     FREEYSIPRKSIKAQAYWVA"
     gene            9252..11657
                     /locus_tag="VFC01_25865"
     CDS             9252..11657
                     /locus_tag="VFC01_25865"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZN83077.1"
                     /translation="MPSSSAQHNGQSDARADTVRRDILPIPDVQHIGLTTYDAKDPDT
                     SYPPIRDVRPPEGAPNVVVILIDDVGYGASSAFGGPCQTPNFEKLAARGLKYTRFHTT
                     ALCSPTRQALLTGRNHHSVGMGGITEIATAAPGYSSVRPNTKAPLALTLQLNGYSTAQ
                     FGKCHEVPVWQTSPAGPFTAWPSGGGGFEYFYGFIGGENNQYDPALYEGTTPIEPPKT
                     PAEGYHLTEDLADKAINWGRQQKALLPDKPFFMYFAPGATHAPHHVPAEWTEKYKGKF
                     AHGWDKQREITFERQKELGVIPPDAVLTPRHAEIPAWDDMDPDLKPVLEREMEVYAAF
                     MEHTDHHVGRLLDALEPVMDNTLVYLMIGDNGASAEGTLQGAFNEMANFNGMAAIETP
                     EFMMSKLDEFGSEASYGHYAVGWAWAMDAPYQWTKQVASHWGGTRNGTIVSWPAGITD
                     KGGLRNQFTHVIDVAPTVLEAAGIPEPTFVNGVMQSPYEGTSMRYSFNDAQAPERHET
                     QYFEMFCNRGIYHKGWSAVTKHRTPWEMTGAAMPAFDDDVWELYDGNTDWTQSKDLSK
                     ENPEKLHELQRLWLIEATKYNVLPLDDRQTERFLPDMAGRPTLIKGNTQLLLGGMGRL
                     SENSVVDIKNKSFAVTSELEVPADGAEGVIIAQGGRFGGWSLYAKDGRAKFYYNVLGI
                     KSYAIDATPPIPSGTTQVRMEFAYDGGGMGKGGDVTLYYDGKEVGNGRVDMTQGAVFS
                     ADETTDVGRETGTTVSPDYTAHTSGFSGTVNWVQIDLGDDAKDADHYIDADERFRIAM
                     ARQ"
     gene            complement(11663..12247)
                     /locus_tag="VFC01_25870"
     CDS             complement(11663..12247)
                     /locus_tag="VFC01_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN83078.1"
                     /translation="MPGDWLAARRTEVAADRILDAAGELFAQQEAATVGMHEIASAAG
                     CSRATLYRYFENREALYTSYVHRESYRLYREMTEQINSVVDPRERLIEGMLASLRNVR
                     ESPALASWFSTTQRPIGAEMAEQSEVIKALTEAFVISLGPDDPELVAHRARWLVRVMT
                     SLFLFPGHNEDDERAMLEEFVVPIVLPNQPERLG"
     gene            complement(12247..13488)
                     /locus_tag="VFC01_25875"
     CDS             complement(12247..13488)
                     /locus_tag="VFC01_25875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN83079.1"
                     /translation="MTATLSHGSPVRFQLADADTWPNPWPMYAALRNHDPVHHVIPPD
                     KPDDDYWVLSRHADVWEAARANDTFSSAQGLTVNYGELELIGLQDNPPFVMQDPPVHT
                     EFRKLVSRGFTPRQVEAVEPKVREFVVERIERLRANGGGDIVAELFKPLPSMVVAHYL
                     GVPEEDRNQFDGWTEAIVAANTTSGGVAGALGGLGDALGGMMAYFTALIERRRTEPED
                     DTVSHLVAAGVGADGDIAGVLSILAFTFTMVTGGNDTTTGMLGGTAQLLHQRPDQRRL
                     LVDAPELIPDSIDEFLRLTSPVQGLARTVTRDVTIGDTTIPEGRRVLLLYGSANRDER
                     EFGENAAELDVERRPRNIMTFSHGAHFCLGAAAARMQSRVALTELLARCPDFEVDESG
                     IVWAGGSYVRRPLSVPFMVRS"
     gene            complement(13601..14968)
                     /locus_tag="VFC01_25880"
     CDS             complement(13601..14968)
                     /locus_tag="VFC01_25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZN83080.1"
                     /translation="MSTEALAALIAELPEGVVVTDPDIVASYRQDRAFDPGAGTALAV
                     VRPRRTEEVQTVMRWATKHKIAVVPRGMGTGLSGGATALTGGIVLSTEKMRDITVDPV
                     TRTAVAQPGLLNAEVKKTVAEYGLWYPPDPSSFEICSIGGNIATNAGGLCCVKYGVTT
                     DYVLGLQVVLADGTVVRLGGPRLKDVAGLSLTKLFVGSEGTLGVITEVTLRLLPPQHV
                     ASTVVATFDSVEAAASAVVTITGKIRPSMLEFMDSAAINAVEDKLRMGLDRNAAAMMV
                     AATDDRGPSGVEDAEFMAKVFTEHGAKEVFSTSDPEEGEAFVAARRFAIPAVELKGSL
                     LLEDVGVPLPALADLVGGIAKIAANRDVMISVIAHAGDGNTHPLIVYDSSDAAMAERA
                     QKAFGEIMDLAVGLGGTITGEHGVGRLKRPWLAGQLGPEAMELNQRIKRALDPDGILN
                     PGAAI"
     gene            complement(15055..17685)
                     /locus_tag="VFC01_25885"
     CDS             complement(15055..17685)
                     /locus_tag="VFC01_25885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HZN83081.1"
                     /translation="MRLTWPLIGRSEEMRTIAAVISDPGVAGILVSGAAGVGKSRIVR
                     EGLSSAASHGFEPRWAVGTMSARKLPLGAFAPWAGSAATDRLQLVRGVIDAVTSAPPG
                     TKVVLGVDDVHLLDELSTFVLHQIVLREAAKVVLTVREGERVPTEIYEIWRGAQFGRL
                     DLQPLSKDEIAELLAAALGGPVDADAATRLWKLTRGNALYLRNIVEQEIADGRIRQQN
                     RYWQWIGEPIMPMGLVELIEARFGALSPPTIEVIDALAVGEPLVMTTLRRIADPDAIE
                     EAHLRGLITVDEVDDELEVRLAHPLYGEVRRSRSPETRLRRLRGLIAAELASAADADD
                     MRSVVRRASLSLDSDLTPDTDLLLKAGSGAVWLGDLRLADRLAKAAIRADGSPRAYFL
                     RAHALSWLFAGQEADAVLAGLPISELTDEDRGRLAYLRASNMLWAIGDAARAKEIIDG
                     AGRSAAPAARIWIDAFLTAYWFAVDQPQQVIETSKSLVLEDLPAVAGAETAWALTVVH
                     ADAGATAKAVSVANTGYAVASRAFDSPHMRFNIADGHISALLLAGQMAEGFNVAQRVY
                     EQGADMPGAAQLLGAAVAGRAALFAGRMDSACALLGKAAPALSASGNAKGWGFRYHIP
                     YATALAMRGSTDDAAAVLAGLDEIRRPFRALNYERSLARAWLVAGQGALTEAIAMMLS
                     AAETAAANGQFAAEVFCLQTAVQFGDYSCASRLGELETMVEGPRAKLAARFATALSAA
                     DGPELSAVSEEFEQMGDLAAAVDAAAHACISFRNSDLRGSALGCSTRAEALAQRCGGI
                     STPALREVSEPLPLTAREREVVRLIGSGLTSRDVAERLHLSLRTVEGHVYRAMTKTGS
                     ATREDLIKLLFPDGRRDMGS"
     gene            17833..18351
                     /locus_tag="VFC01_25890"
     CDS             17833..18351
                     /locus_tag="VFC01_25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN83082.1"
                     /translation="MLAALDLFAEHGVSGTSLQMIADAMGVTKAAVYHQFKTKDEIII
                     AVTEMQLARLEAALEDAEAADDRPQARELLLNRVIELAVEQRHMVGVMQSDPVIIRFL
                     AEHKPFQAFLERLYAALIGDDAGPETLVQAAMLSGAIGSAVKHPLVAHLDDPTLRSHL
                     LRLTRRLVDLPR"
     gene            complement(18393..19667)
                     /locus_tag="VFC01_25895"
     CDS             complement(18393..19667)
                     /locus_tag="VFC01_25895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN83083.1"
                     /translation="MTNGKRGPLFLILFGALMAGAGNGISIVAFPWLVLQRNGSALNA
                     SIVAMAGTLPLLAATLIAGAAVDYLGRRRVSMISDALSALSVAAVPVLALMFGVHVIN
                     VAVLAALAALGAFFDPAGMTARETMLPEAATRAGWTLDHANSVYEAVFNLAYIVGPGI
                     GGLLIATLGGVNTMWVTASAFGLSIVAIGALRLEGAGKPDRAALPEGVWAGIVEGLRF
                     VWNNKVLRTLAIVDLTATGLYMPMESVLFPKYFTDRNEPAQLGWVLMALSVGGLVGAL
                     GYAVLSKYSSRRAVMLTAVLTLGVAMTIIAFLPPLPVILVLCAVVGLVYGPIAPIYNY
                     VMQTRAPQHLRGRVVGVMGSLAYAAGPLGLIVAGPLADSAGLHATFLALSLPMLALGL
                     VAIFLPALRDLDSAGQMKGPPTPGATPSSGTA"
     gene            19773..20546
                     /locus_tag="VFC01_25900"
     CDS             19773..20546
                     /locus_tag="VFC01_25900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZN83084.1"
                     /translation="MFDSPVLPGSGWPGDPATSRTAVAETPAHVASMAAAVRTLEELD
                     AEVSVCRACPRLVEWREQVATDKRKSFADQPYWGRPIPGWGSARPRVLIVGLAPAAHG
                     GNRTGRVFTGDRSGDFLFAALHRARLANQATCVDAADGLVLNDTRVAAAVRCAPPANV
                     PTPAERAACAPWLDAEWRLIGSEVQVIVALGAFAWRAALQMIGSVPTPAPKFGHGATA
                     TLGDVTLIGCYHPSQQNTFTGKLTPQMLDDIFSKAAEVH"
     gene            complement(20617..21315)
                     /locus_tag="VFC01_25905"
     CDS             complement(20617..21315)
                     /locus_tag="VFC01_25905"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HZN83085.1"
                     /translation="MLAMRPVASTSPLSNAERLRGTLELVLWKLSAAGERLVKHPRVR
                     ELYPEYLFTLHSVIRASVPLMETARDRARSISAADPVAARLVEYLDEHIEEERDHDEW
                     LLEDLASLGVDRSTITARVPSPTVARAVGAQYYWVLHYHPVALLGWIGLLEGYPPTPA
                     TIDELMARTGYELQAFRTLSAHAELDIGHGDEIFATLDQLPLTAEQSEVIGLSAMSSV
                     DLLAHALDEVTRSA"
     gene            complement(21334..21639)
                     /locus_tag="VFC01_25910"
     CDS             complement(21334..21639)
                     /locus_tag="VFC01_25910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83086.1"
                     /translation="MTSPDVGVNEAQLKALVVFESIIKDAEGRQEWLAAPRGEKEAVF
                     NRHRPGDLGDADYSQLPEPVRQLFETLSDSELALLSDINTTLVSAGLSLPQYPLMAH"
     gene            21731..24853
                     /locus_tag="VFC01_25915"
     CDS             21731..24853
                     /locus_tag="VFC01_25915"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN83087.1"
                     /translation="MAADIRKVVTVLFCDITGSTTLGEALDPESLQRILSRYFGEMKA
                     IVERHEGAVEKFIGDAVMAVFGLPSVHEDDALRAVRAAVEMRDALATLNAEFAVKWGV
                     TLAIRTGINTGEILAGEPGEGQALVVGDAVNTAARLEQFAEPGQILMGESTYWLVHDA
                     VTAEPVGPLGLKGKSSAVSAWQLFAVVAHAPGWSRRLDSPIIGREQEIRRLENAFERT
                     VENGACELITVMAPAGVGKSRLIAEVLSRLVSRATILQGRCLPYGDGITFWPIASLLM
                     EAIEMTERDTEMTARRRLSELLGQTADDQEDAELVCDALAPLLGFGTEAVGIRETFWA
                     VRRLLEHLAARRPIVVVFDDIHWGQPTFLDLVEYLTDWIRGVPVLLVCQARPELLDTR
                     PRWVSTKSNASVMTLQPLTEAQTKTLIRGLTGGADLPLEARTRIAAVAEGNPLFVEET
                     VRMLVDHAVLQSRDGRWLVSGDLSAIAIPPTIHALLAARLDRLEPDQRMVIEQASVIG
                     RSFWWGAVSELAPKEIQQHVGEYLQSLVRRELIEPDESEIRGEDAFRFTHILIRDAAY
                     HGVPKATRAELHERLAGWIAAHTRELAGKYETIVGYHLEQAHHALIELGPQSDRSSEL
                     ANRAASTLAAAGDRALARGDMPAAVNLLSRAAALLSPQDGRRLELLTEVAFALIEIGE
                     FDRVVGVVEELNTAAKETNDAGLQCEATIVGLWIRLFTNPDGWAADATKQAKRAITTF
                     GELEDDAGLARAWSLLGLTQMMTARFAAAEDSWWRAVGHAQRAGSRRDVLESLSWIPG
                     ALWAGPTPAVEGIARCRDVLEQARGDKKAMASALFSQGFLEGCVGQFDDARQLFAQAR
                     ALLEEVALPVWIAGPLTQCVGWTELLAGNAQGAERELRAGWETLQAIGEVTLLSTVAG
                     LLAEAVYAQERYDEAEHFTRLSEESAGPEDVYSHALWRSVRSKVLAWRGELDEALLLG
                     REAVGFAESSDSPLLRWHTLVSRAAVLRIANQPQGAEEDLREAADLAEQKGNVVAARI
                     AREALGRGAC"
     gene            24908..25945
                     /locus_tag="VFC01_25920"
     CDS             24908..25945
                     /locus_tag="VFC01_25920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN83088.1"
                     /translation="MRLSVLDLVPVRTDQTTSDALAATTQLAQTADRLGYTRYWLAEH
                     HNMPAVAATSPPVLIAHLAAQTSQLRLGSGGVMLPNHAPLAVAEQFALLEAAHPGRID
                     LGIGRAPGSDPVTSLALRGAAGRDDRDIEAFPEYLDDVVALMSARGVRVPIPNQRYIL
                     KATPAAATEPKLWLLGSSMYSAHLAAAKGLPYVFAHHFSGQGTAEALEVYRSEFRPGE
                     LASEPVTFLTVNAVVAETRDEAMALILPNLQMMGRLRTGQPLGPLDLVEDAAEVTMAP
                     QAERIAEGALRRAVVGTPTEAAEQVRALADEFGVDEVMVNPVASAYRGTDPASAPARD
                     KTLELLAKELF"
     gene            complement(26015..>26405)
                     /locus_tag="VFC01_25925"
     CDS             complement(26015..>26405)
                     /locus_tag="VFC01_25925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019739141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN83089.1"
                     /translation="YKGTNGRIGHTIPGGPSQLILHTTGAKTGQKRANSLSYARDGDD
                     YLVVASKGGDPKAPGWYHNLKANPKVEINVGPKRFGVTAKPVLPDDPDYQRLWPIVNN
                     MRGNKNRYIGYQKRTSRPIPVVVLTPA"
BASE COUNT         4589 a         8622 c         8731 g         4463 t
ORIGIN      
        1 ctggccgccg tgtgcgtctt gcccgtgccg ggtgggccgt gcaccgcgag gtacgacgag
       61 tcgaggtcga gcagggcggc cgtgatgtct gttgtcgtgt cgccgctatg cggcagcgat
      121 gcgccgctgc gggtgcgtgg ggtacgccgc cgcaggatgt cgacgatcgc ggtgtcgggc
      181 agcgcgggca ggccggcggc gacgctcgcg gccgcggtgt cgatggactc gcgcaacttg
      241 cccgtcggga agggcctgcc cggtgtaagc gcgaacggca gttccgcgaa cgtgccgccg
      301 tctttgggct cgcgttcgca gatggtgacc tcggtgggga cgttggggtc gtcgacgccg
      361 gtgatggtgg cgttgcccgc ggcgcgccgg tcggtgttct cagtgagccc agcgggcgac
      421 ggcgggtcgt agagcgcgaa cacgttagtg tccagcccac ccgcggccag cgttccggtc
      481 agcttcaccc agcgctgctg ctttcgtgcg cgcgacgacg gcacgtgcca gtcctggacc
      541 acttcggcct tgtcggctac gaacacgtcg ctggtgtcgc cccactcgtc gacggggttg
      601 ttcagccgat cgaaatgcgc ccaccagaac ggcttgtcct cgcgacggtg atagccacgc
      661 gcggccgcga acaacgcgac tgccgtctgc tccgggctgc gttcagcgac gccgtcgccg
      721 gcgaacgtca taaacgtgcg ggcgagctcg tcggtgtcct cgacctcggt gccgtcgccg
      781 acgggctgag ggcccagcgg tggcacgctg gattcgatcg cgatcttgat caaccagtcg
      841 cgaagcctgt gtgtcgagcg gcagtcgtag cggttgtagt cctcgatctc cttgagcacg
      901 ttggcggctt cgtcgtttcg gccatcggcg cgcaattcgc agtagcgcgc gtacatggtg
      961 atcgactccg tggcggtggt gacgtcgccg gtgcgcagct cggagcccat gtagagcggt
     1021 tcgagcgact tgaggctgta gttctcggtc ccgacgcgaa tgctcttgcg caccaacggg
     1081 aaaagatcga cgagcacacc gctgcgcagc aggtcgtcga cctcgtcctc cccgacaccg
     1141 tagcggcccg cgagccgtag cagcgcggtc ttctcgtacg acgcgtagtg gtagatgtgc
     1201 attttcggat agcgcttgcg acgcttgcgg accatcgtga ggaagtcgac gagcgcctgg
     1261 cgctcgctgg cgcggtcgtg cgcccacagc ggcttgaagc cgccgccgat gtcaagcacg
     1321 ccccacatgt actccaggcc ccactcgtgg ccgtcggcgg tccacagtgg atcaccctcg
     1381 aagtcgaaga acaggtcgcc cttgtcggga tcgggcagca ggttcagcgg ctgtgggtcg
     1441 gcgatctcgt acggcggctt gccgtcgacg cgcggtgcga tctgcaggcg tgcctgcttg
     1501 gtcaacgacg tcaccgtgcg ggcgggtagc tccgggaccg ggccgtcgtg tcgcgtgagc
     1561 gccgacaacg tggtgatgcc ggcttcgatc agccgggccc gctggctgac ccgcatgcca
     1621 gccacgagaa ggaggtcgtc ggtggcgcgg acctgcagtg cgcattcggg gcagcgaaag
     1681 catgcccgga cctcgtcgtt ctcccacgtc accggggcgc cgctggccaa gtggtcgtcg
     1741 agcaggcgct gcagcgctgc gcggcgcggc agatacaccg gcagcaactc gtcgacgcgg
     1801 taggtcgcag tcgcgccgtc accgagcacc agttcgacct cggggcttac cgacaccccc
     1861 gctgcggtga gcgtgtcggc gtaggccgcc agctgtagca gggcctcgac cttgaccgac
     1921 cgggccagct tcgtgtcgcg cagccggtac tgctctccgt cgaacagcag gaagtccgcg
     1981 aagccgacga accggccgtc gaacatggcg gcctgataga tcaccggcgc gcggcgctcg
     2041 acggcacgca tggtctgttc cgcggcggcg gtcaatccgg cgacggtgta cggcggcctg
     2101 ccgatgatcg cgacggtgtc gtcggcccgc agctcgtcga ggtggcgttg ctcgtgctca
     2161 ccgccgagtt tggctgtccg cgcgagcagt tcgtcctcga cagcgacggg gggtccccag
     2221 ccgagtcggg cgtcgaaaga ccgcagcagc gcatactcgc agcgcgctgc ggcggcgaga
     2281 tcggatgcgc tgtagacgac gcgatcgtcg gcgacgaaca cgctgccact gtatgtgagc
     2341 cagccgacac ctcactgtca ggcgcgaacg tgggttaccc gcacgcaaaa tccggcgtcg
     2401 gcgtaacaca agcccacact cgtccatccc cagagtggca gttatccaca gaaccgcgct
     2461 tcagacgggc cgcgacagcc gattgcgtgg gaccgcgcgc tgacggtaag cgcatgaatg
     2521 aagcatcgtt gccgttcatc ggcactgaag cagtggggtc gggcaaggtc accaggcgaa
     2581 cgttggccag cggccacaac atgatctatc gcaacatcta cttgccggac ggcgtggagc
     2641 tcacaccgga tagacgcgca gtggcggcat ggctgtggtc ggggcgaaat gccaccatcg
     2701 ccggaatggc cgccgcagcg ctgcatcgat ctcagtggat tgatttggag ctaccggccg
     2761 agttgatccg caacgaggcg tgcaaggtcg acgggatcgt gattcatcgg gggcgactcc
     2821 gggatgacga gatctgtgtc gtgcagggaa tgcccgtgac gacgccggca cggacggcgt
     2881 tcgaccttgc tcggcgggat cggctcgaga cggcgatcat ccgggtcgat gcgttggcga
     2941 acgctactgg cctcaagccg caagatgtcg agcgtctcgc cgaaagccgt cgcggggctc
     3001 gcggcatagt gcagttgcgg cgtgttgtag agctgatgga cggtggcgcc gagtcgccgc
     3061 aggaaacccg gacacgcctt ttgttgatcg ccgccgggtt tccgaagccg caaacgcaga
     3121 tcgttgtcgt cgacgagtac ggcggcttcg tcggtcgcgt cgacatgggc tgggcggaat
     3181 ggaaggtggg cgtcgagtac gacggtcctc aacactggga ggaccccgac cagcatgctc
     3241 gcgacatcga ccggctggca gatctcgccg cccagggctg gttgatcatc cgggtgagtc
     3301 gagatcttct gcggtaccga ccgcatgtat tcctcgcccg cgtgcgggat gctatgcgag
     3361 ccgcgggttg gccccactgg gacaagatcc gactcgacgc gcgcatttcg tggctcgcct
     3421 actagccgtc gagtgtgggc ttaacgcacg cctactcaac gaaatgcgtg ccggtaaccc
     3481 acgttcggca atgctcagcc gagcgtgttg ccgatcagct cgacaccgtt gccgttccac
     3541 cggaacttca ccacactgtc gaggccctcg ataccgcccg aatagcgcag cgcgatcgtg
     3601 tcgccggtcg tggcggactc gtcgatgcca ttgaagccgt aggtgtccgg cacgccggtg
     3661 ggaatgaact tgccgaggtg gaacagcacg gcgcgggtgt tcgggttgtc ggagttggtg
     3721 ttggccttga cgatcaccgc cgacaactgt gcgcattcgg tgtagttgcc agccagcggc
     3781 tcagggttcc acgcctgatt gcttcgcggg tcgcggggca gttcggagac cgccttgatg
     3841 atctcgggtg cggccaggtt gaccgcgcac gggtcggccg ccgcgctggt ggtgggcgcg
     3901 acgatgggcg gcggcggtgg caacgacagc ggggccgtcg ccggggcgac cgacgggacc
     3961 gtcggctccg gcgtcttgga cgccgtcgag tcgcttgaac cgcagccggc gacagcgatc
     4021 gtcgccgcca caacaaggac ggcccatttc acaactgggc aacctaccgc caacgtcacc
     4081 ggccgtcgga caggcgcgcg gcggggaatc gcatcgtcaa ccactagact tcctgcgaga
     4141 tgacgtcccc cgaaccggat gcgaccagcg ccgatctgac cttcgcggac ctgcagattc
     4201 acccctcggt gctgcaggca gtcggcgacg tcggatacga gtcgccgtcg gccatccaag
     4261 ccgcgaccat tcccgccatg ttggccggct ccgacgtcgt cggcctggcc cagaccggca
     4321 ccggcaagac cgccgccttc gccatcccga tcctgtccaa gatcgacccg acgaacagga
     4381 cgacgcaggc cctcgtgctg gcgccgaccc gcgaactggc gctgcaggtg gccgaggcat
     4441 tcggccgcta cggctcgcac ctgccgcaga tcaacgtgct gccgatctac ggcgggtcgt
     4501 cctacggccc gcagctggcc gggctcaggc gaggcgcgca ggtggtcgtc ggtaccccgg
     4561 gccgggtgat cgaccatttg gagaagggca cgctcgatct cacgcatctc gactacctgg
     4621 tgctcgacga ggccgatgag atgctgcaga tgggcttcgc cgaggacgtc gaacgcatcc
     4681 tcgccgacac ccccgagtac aaacaggtcg cgctcttctc ggcgaccatg ccccccgcga
     4741 tccgcaagct caccaccaag tatctacacg acccggtcga ggtcaccgtc aaagcgaaaa
     4801 ccgctacggc ggaaaacatt tcgcagcgct acatccaggt ggcaggcccg cgcaagatgg
     4861 acgcgctgac cagggtgctg gaagtcgagc agggcgacgg catgatcgtg ttcgtccgta
     4921 ccaaacaggc caccgaagaa gtcgccgaga gactcaaggc gcggggcttt tccgcggccg
     4981 cgatcaacgg cgacatcccg caggcgcagc gcgagcgcac catcgccgcg ctgaaggacg
     5041 gcagcatcga catcctcatc gccaccgacg tcgcggcccg cggactggac gtcgagcgga
     5101 tctcgcacgt gttgaactac gacatcccgc atgacaccga gtcctatgtg caccgcatcg
     5161 ggcgcaccgg ccgggccggc cggtcgggtt cggctctgtt gttcgtcacg ccgcgggaaa
     5221 ggcacctgct caaggcgatc gagaaggcca cccgctcaaa gctgatcgag gcggagttgc
     5281 cgtcggtcga ggatgtgaac gcccagcggg tcgcgaagtt ccgtgactcg atcaccgacg
     5341 cgctcaatgc gccgggcgtc gacctcttcc gaagcctgat cgaggactac gagcgcgaga
     5401 acgacgtacc aatggccgac atcgctgccg cgctggccgc ccaggcccgc gatggaaagc
     5461 agttcttcat ggtcgagccg ccgccggaga agcggcgtcg agatgagcgc ccagacaagc
     5521 acgaccgcga ccggccgaag cgcaaaccgg gacagggctt cgcgacgtat cgcatctcgg
     5581 tcggcaagcg gcacaaggtc agtccgggcg cgattgtcgg cgccattgcc aatgagggcg
     5641 gcctgcatcg cagcgatttc ggacacatct ccattcggcc cgatcattcg ttggtggagc
     5701 tacccgtcga gttgccgcgc gagactgtta aagccttgga gcgcacccga atccagggaa
     5761 tcctgatcaa tctgcagccc gaccggccgc cacggaaggc cgggaagaac agccgcaaat
     5821 cgaaatgacc ctgtccaagg acctggatgc gcagggcggc ctggagtcgg tcagcaagcc
     5881 cgaacgcgtt gcgtcactga ctggtatccg cgcggtcgcg gcgctgctgg tgatgtttac
     5941 gcatgccgcc tacaccacgg gtaaatacac gcacggctat gtcgggttgg tgtattcgcg
     6001 gatggagatc ggcgtgccga tcttctttgt gctctcggga ttcttgttgt tcggaccgtg
     6061 ggtcagggcc gccgcatcgg acggcgaacc accatcggtg cgccgctatg cctggcaccg
     6121 ggtgcgccgc atcatgcctg catacaccgt caccgtgctc gtggcgtatg ccgtctacca
     6181 cttccggact gccggaccca acccaggtca cacgtggttg gggttgttcc gcaacctgac
     6241 gctgtcgcag atctacaccg acaactatct gtattcctat ctgcaccaag gcctcacgca
     6301 gatgtggagc ctcgcggtgg aggtcgcgtt ctacgtgatg ctgccactgt tggcgtacct
     6361 gctgctggtg gtgttgtgcc ggcggcgctg gcgaccgggg ttactggtgg ccggcctgtc
     6421 gggcctggcg ttgatcacgc ccgcgtggtt gatcctggtg cataccaccg acttcctgcc
     6481 cgacggtgca cggctgtggt tgccgaccta cctctcgtgg ttcatcggcg gcatgattct
     6541 ggcggtcctg cagccgatgg gcgtgcgagc gtatgcgttc gtgtgcgtgc cattggcggt
     6601 ggtctgctac ttcatcgtgt ccacgccaat cgcgggggcg cctaccacgt cgccggccga
     6661 actgcgtgag gcgctgttca aggcggcctt ctacgccgtg atcgccacat tggccgtcgc
     6721 gccgctggcg ctgggcgaca gcggcttgta cgcgagattc ctcgccagcc ggccaatggt
     6781 gttccttggc gagatctcct acgagatctt cctgatccac ctggtcacca tggagttggt
     6841 gatggtcgag atcctccact ggcacattta caccggcaac gtggcgattc tcttcctcgt
     6901 gacgctcgtg gcgacggtcc cggttgcatg gctgctgcac cgggtcaccc gcgtccgccc
     6961 gtgactatgc gcggcgtcgc atgacggcaa gcgcgaccgc caccacaccg attgcgatgc
     7021 cgccggctgc cagccaccgt gccgtgctgt cggcaggcgg cgacgattcc gccgtcatgg
     7081 cgtggtgttc atcggccggt tcgccgctca aagccaatgt gggcaccggg tattcgggtt
     7141 ccgtgccact cgcaggcgtt ggctggtccc acttcacgac ggtgccgtcg ctgtaggttt
     7201 gagtggcggg gaagctgacg ctgtcggcct tgggcaacag gacctccacg cggaacaacg
     7261 cgaactggtc cggtggaaca ccagccccag aagcagcggt ccaagtgacc gttttgaccg
     7321 tgcccgctgc ggcgtcgcgg tcgagtcgga cggtccaacc gggcatctgc tgcgtgctca
     7381 ccgaagtgag attgggcagc gagacgctga gttcggttgt cgccgcacct ttctcggatt
     7441 cgttgggcac gttgaaggtc aggatcgcct ccgagcccgg ttgggcgtgg tcggcctcga
     7501 cgtgcacgtg tgcccacgcc ggcgccgccg tcaccaccgt gagcgcagcc aacgctgcgg
     7561 tggccatcgc cacacggcga atacgagtcg aatagctgtt gtgtgtcata tcaatttcac
     7621 cttttcggta gtagtggtgt ttgcggtccg gcgcttgacc gcgccgacga ggatgtcgac
     7681 gagcaggaaa accagaaggc tcagcccgaa cagcgtttgg gaaatgtgct ggtttacagc
     7741 gcaactgaag cccgccctcg attggcgggg cgggcctacc taaagcggag cgctaagcgc
     7801 tcacgacgga cacctgtggc ggcgcccgca ttcccgaacc ggcacgcggc agaacaggcc
     7861 gcgcgacaac gacattcgag ggccgccacg atcgcccctc gggccggagc caaccggcag
     7921 cgaagattcg cagccagcac aacaccgacg cgcacaccgc gtacaggtac tcgaccgcgc
     7981 tgatcaacag tccgagcccc agcgcggcga gtacgtgcag catcagcatc ggcgtggtca
     8041 acaaccaacc agcgtgatgg ccaccgccga cactcagcgt tacgtgtccc agcgcttggc
     8101 ccacgcccag cgcaccgatc accacaaagg cgtgcgcgtg gcggccctca agcttgatcc
     8161 cgccgacggc cgcgccgacc gtcgcgcaca cgaccagcag caggatcaac gcactgcctg
     8221 ttggaacgcc accgcctgcg ccggcatgtg cggcggcggt taccagcgcg gcgcacagac
     8281 cgacgagccc gccgcgcaaa aaggcggggt acgtcggcct gctcatgcgc cgaaccttac
     8341 cgccggggct actacatccc ctagtagacc cgatagcata tgagttaggt taagctgccc
     8401 taaataatcg acgggcaaac atgtccgaca ggatgccatc gcgcgggttc tccggcgccg
     8461 tgctcaagct gctacgcgct ggcgattacc agctgaccgt gaccggccgg cgcgagatca
     8521 gcccgcacta cctgcggctg agcttcgacg cgggcggcat actgaccgat cggccggtgc
     8581 acccgacgat gtggatccgc atgtggttcg ccgacggcga caagctgcat cagcgcggct
     8641 acaccctggt cgaccccgat ccggcggccg acaccgtcga catcgagttc gcgctgcacg
     8701 acagggtggc ctcgcggtgg gcccaagatg cgcggccggg cgacaccatc gaggtgacgg
     8761 tgttgggcag caatttcacg ctgcccgagc cggcgcccaa gggctatgtc atcgtcggcg
     8821 acaccgcctc gctgcccgcc gtcaattcgt tgctgaccgc gatcggtaac accccggcca
     8881 aggtgtttct cgaagccggc cacgacgacg acaagcaact gccggtcgcg cgcagcgcgg
     8941 acgtggtgtg ggtggaccgc aagaacggcg gcgaggcact cgtgcgggcg gttggctcat
     9001 cggcgttcga cgccgccgac cacttcggtt gggtggcatg cgacaaccga accacccgcg
     9061 cggtggcgaa ggtgttccgc gaggagtact ccatcccgcg caagtcgatt aaggcgcaag
     9121 cgtattgggt ggcatgaccg gttggtgaag ccaatttggc gctggcgtta aaacgccgcg
     9181 acggcgggca cggccgcata gtgttagccg tcttggaccc aagcagcgtg gttcgagcgg
     9241 agaggacagt catgcccagc tcgtcagcgc aacataacgg tcagtcagac gcacgagcgg
     9301 acaccgtccg gcgtgacatc ctgcccattc cggacgtaca gcacatcggt ctgacgacgt
     9361 atgacgccaa ggaccccgac accagctatc caccgatcag ggatgtgcgt ccaccggagg
     9421 gtgcacccaa cgtcgtggtc atactcatcg acgacgtcgg ctacggtgct agcagcgcgt
     9481 tcggtgggcc atgccagact cccaatttcg agaagctcgc ggcgcgcggt ttgaagtaca
     9541 cccggttcca caccaccgcg ctgtgctcgc cgactcggca ggcgctgctc accggtcgca
     9601 accaccattc ggttggcatg ggcggcatta ccgagatcgc caccgccgca cccggttaca
     9661 gctcggtgcg gccgaacacc aaggcgcccc tggcgttgac gctgcaactc aacggatatt
     9721 ccacggcaca gttcggcaag tgccacgagg ttcccgtctg gcagaccagt ccggcgggac
     9781 cgttcaccgc atggccgtcc ggcggtggcg gtttcgagta cttctacggg ttcatcggcg
     9841 gcgagaacaa ccaatacgac ccggcactgt atgagggcac gaccccgatc gaaccaccga
     9901 agacgcccgc ggagggctat cacctgaccg aggatctggc cgataaggcg atcaactggg
     9961 ggcgccagca gaaggcgctg ctgcccgaca agccgttttt catgtacttc gcgcccggcg
    10021 ccacccacgc gccgcaccac gtccccgcgg agtggaccga gaagtacaag ggcaagttcg
    10081 cccacggctg ggacaagcag cgcgagatca ccttcgagcg gcagaaggaa ctcggcgtaa
    10141 tcccacccga cgccgtgctg acgccgcgcc acgcagagat cccggcctgg gacgacatgg
    10201 atcccgatct caagccggtg ctggagcgcg agatggaggt gtacgccgcg ttcatggagc
    10261 acaccgacca tcatgtgggc cggttgctgg acgccctcga acccgtcatg gacaacaccc
    10321 tggtctacct gatgatcggc gacaacggcg cctcggcgga gggcactctt cagggcgcgt
    10381 tcaacgagat ggccaacttc aacggaatgg ccgccatcga gacgcccgaa ttcatgatgt
    10441 ccaagctcga cgagttcggc agtgaggcgt cctacggcca ctacgcggtg ggttgggcgt
    10501 gggcgatgga tgcgccgtat cagtggacca aacaggtcgc ctcgcactgg ggcggcacgc
    10561 gcaacggcac gatcgtgtcg tggccggcag ggatcaccga caagggtggg ctgcgcaatc
    10621 agttcactca cgtcatcgac gtcgccccca cggtgctaga ggcggcgggc attccggaac
    10681 cgacgttcgt caacggcgtc atgcagagtc catacgaggg caccagcatg cggtacagct
    10741 tcaacgatgc acaggcaccc gagcgtcacg agacccagta cttcgagatg ttctgcaaca
    10801 gaggcatcta ccacaagggc tggagcgcgg tgaccaagca ccgcacaccg tgggagatga
    10861 ccggtgcggc catgcctgcc ttcgacgacg acgtctggga actctacgac ggcaacaccg
    10921 actggaccca gtccaaggac ctttcgaagg agaacccgga gaaacttcat gagctgcaac
    10981 ggctctggct gatcgaggcg accaaataca acgtgcttcc actcgacgac cgccaaaccg
    11041 aacgctttct ccccgacatg gccgggcgtc cgaccttgat aaagggcaat acccaactgc
    11101 tactcggcgg gatgggtcgg ctgtcggaga acagcgtcgt ggacatcaag aacaagtcgt
    11161 tcgccgtcac ctccgaactg gaggtgcccg ccgacggcgc tgagggcgtg atcatcgcgc
    11221 agggcgggcg gttcggcggg tggagccttt acgccaagga cggtcgggcc aagttctact
    11281 acaacgtgct tggcatcaag tcatatgcca tcgacgccac tccgcccatt ccgagtggaa
    11341 ccacccaggt gcgaatggag ttcgcctacg acggcggcgg gatgggcaag ggcggtgacg
    11401 tcacgctgta ctacgacgga aaggaagtcg gcaacgggcg cgtcgacatg acacagggcg
    11461 ccgttttctc ggcggacgaa accaccgacg tcggacggga gacggggacg acggttagcc
    11521 ccgactacac cgcgcacacc agtgggttca gtggcacggt caactgggtg cagatcgacc
    11581 tcggcgatga cgccaaggac gccgatcact acatcgacgc ggatgagcgc ttccgcatcg
    11641 cgatggcacg gcaataaccg gactatccca gccgttccgg ctggttcggc agcacgatcg
    11701 gcacgacgaa ctcctcgagc atcgcccgct cgtcgtcctc gttgtggccg gggaacaaga
    11761 acagtgaggt catcacgcgc accagccagc gggcccggtg cgccaccagt tcggggtcgt
    11821 ccggtcccag cgagatgaca aacgcctcgg tcagcgcctt gatcacctcg gactgctcgg
    11881 ccatttccgc accgatcggc cgctgggtgg tcgagaacca cgatgccagg gcggggcttt
    11941 cgcgcacatt gcgcaacgac gcgagcatgc cctcgatgag ccgctcacgc ggatccacca
    12001 ccgaattgat ctgctcggtc atctcgcggt agagccggta gctctcacgg tgcacgtagg
    12061 aggtgtacag cgcttcccgg ttttcgaagt accgatacag cgtggcacgt gaacaccctg
    12121 cagccgaggc gatttcgtgc atgccgaccg tcgccgcctc ctgctgtgcg aacagctcgc
    12181 ccgcagcgtc caggattcgg tcggcggcta cctccgtgcg ccgcgcggcc agccagtccc
    12241 cgggcatcag gacctcacca tgaacggtac cgacagcggt cgtcgcacgt agctaccgcc
    12301 ggcccagacg attccggact catcgacctc gaagtcgggg cagcgcgcca gcagctctgt
    12361 caacgcgacg cgggattgca tacgggcggc agcggcacca aggcagaagt gcgcaccgtg
    12421 gctgaacgtc atgatgttgc gcggccgtcg ctcgacatcg agttccgcgg cgttttcgcc
    12481 gaattcgcgc tcgtcgcggt tggccgatcc gtagagcaaa agcacgcggc gaccctcggg
    12541 gatggtggtg tccccgatcg tgacgtcgcg cgtcaccgtg cgggccagac cctgcaccgg
    12601 cgacgtcagt cgcaggaact catcgatcga gtcgggaatc aactctggtg catcgacaag
    12661 tagccgccgc tggtcgggtc gttgatggag caattgcgct gtgccgccga gcattccggt
    12721 cgtggtgtca ttaccgccgg tgaccatcgt gaatgtgaac gccagaatcg acagcacccc
    12781 agcgatgtca ccgtcggcgc ctacgccggc ggccaccaaa tgcgagacgg tgtcgtcctc
    12841 gggttcggtg cgacgccgtt cgatcaacgc ggtgaagtaa gccatcatcc cgccgagtgc
    12901 gtcgccaaga ccgcccaatg ccccggcgac gccgcccgag gtggtgttgg ccgcgacgat
    12961 cgcctctgtc cagccgtcga attgattgcg gtcctcttcc ggcacaccga ggtagtgcgc
    13021 gacgaccatc gacgggagcg gtttgaacaa ctcggcgacg atgtccccgc cgccgttggc
    13081 gcgcaaccgt tcaatgcgct cgacgacgaa ttcgcgaacc ttgggctcga cggcttcgac
    13141 ctgccgcgga gtgaagcccc gcgacaccag cttgcggaac tcggtgtgca ccggcgggtc
    13201 ctgcatcacg aacggtggat tgtcctgcag accgatcagc tcgagctcgc cgtagttgac
    13261 cgtcaggccc tgcgccgacg aaaacgtgtc gttggcgcgg gcggcctccc acacgtcggc
    13321 gtgccgggac aacacccagt agtcgtcgtc cggcttgtcc ggcgggatga cgtgatgcac
    13381 cgggtcgtgg ttgcgcagcg cggcgtacat cggccacggg ttcggccagg tgtcggcatc
    13441 agccagctgg aaacgaaccg gcgaaccatg agacagagtc gctgtcatgt ctcattggta
    13501 cgacacctca ccgcgacgtg tcaatagtca gtctcggtga gcagacgcaa aagtgcccta
    13561 gaacgcctga aaaagggcac ttttgcgtct gctggcgggc tcagattgcc gcgcccgggt
    13621 tgaggatgcc gtcggggtcg agcgcccgtt tgatccgctg gttgagctcc atcgcctcgg
    13681 gtccgagttg gcccgccaac cagggccgtt tcagccggcc gacgccgtgt tcgccggtga
    13741 tcgtgccgcc gagcccgacc gccagatcca tgatttcgcc gaacgccttc tgggcccgct
    13801 cggccatcgc ggcatcggag gagtcgtaca cgatcagcgg gtgggtgttg ccgtcgccgg
    13861 cgtgggcgat caccgagatc atcacgtcgc ggttcgcggc gatcttggcg atgccgccga
    13921 cgagatcggc caaggccggc aacggaacgc cgacgtcctc gagcagcagt gaacccttca
    13981 actccaccgc cgggatcgcg aaccggcgcg cggccacgaa cgcctcgccc tcctccgggt
    14041 cggatgtcga aaacacttct ttcgccccat gttcggtgaa taccttcgcc atgaactccg
    14101 cgtcctcgac gcctgagggt ccgcggtcgt cggtggcggc caccatcatc gcggcggcgt
    14161 ttcggtcgag gcccatccgc agcttgtctt cgacggcgtt gatcgccgcc gaatccatga
    14221 actccagcat cgacggccgg atcttgccgg tgatcgtgac gacggcgctc gcagcggcct
    14281 ccaccgagtc gaaagtggcc accaccgtgc tcgcgacgtg ctgcggcggc agcagccgca
    14341 gcgtcacctc ggtgatcacc ccaagcgtgc cctcgctgcc gacaaacagc ttggtcaacg
    14401 acagcccggc gacatctttc agccgtggtc cgccgagtcg caccacggtg ccgtccgcca
    14461 gcaccacctg aaggcccagc acgtagtcgg tggtgacgcc gtacttgacg cagcacagcc
    14521 cgccggcatt ggtggcgatg ttgcccccga tgctgcagat ctcgaacgac gacgggtccg
    14581 gcggatacca cagcccgtac tcggcgacag tcttcttgac ctcggcgttc aacagcccgg
    14641 gctgggcgac ggcggtgcgc gtcaccgggt ccaccgtgat gtcgcgcatc ttctccgtgc
    14701 tcagcacgat gccgccggtc agcgcggtcg ccccgccgga cagcccggtg cccataccgc
    14761 gaggcacgac ggcgatcttg tgctttgtgg cccagcgcat caccgtctgc acctcttcgg
    14821 tacgacgggg ccggaccacg gccagcgcgg tgcccgcacc cgggtcaaac gcccggtctt
    14881 gccggtagga cgccacgatg tcgggatccg tgacgactac accttcgggc agctcagcga
    14941 tgagagccgc caatgcctct gtgctcatgc ctcgactccc gctcgctccg gtcctcgctc
    15001 gttcctcgct gcgatcctca ctcgcggtcg tcttcgggga ctcatacgtc gatgctacga
    15061 gcccatgtcg cggcgcccgt cgggaaagag cagcttgatg agatcctcgc gagtggccga
    15121 tccggtcttg gtcatcgccc ggtagacgtg accttcgaca gtgcgcaacg acaagtgcag
    15181 ccgctcggca acgtcgcgac tggtcagtcc cgatccgatg agccggacga cctcccgctc
    15241 gcgtgcggtc aagggcagtg gttcgctgac ctcgcgcaga gccggcgtgc tgattccgcc
    15301 acatcgttgg gcgagcgcct ccgcgcgcgt cgaacatccc agcgctgagc cacgcagatc
    15361 gctattacgg aacgagatgc aggcatgggc ggcggcgtcc accgctgcgg cgagatcgcc
    15421 catctgctcg aattcttcag agacagctga caattcaggg ccgtcggcgg cgctcagtgc
    15481 ggtcgcgaac cgggcggcca gcttcgcgcg cggtccctcg accatggttt cgagttcacc
    15541 cagccgagac gcgcacgagt aatccccgaa ctggacggcg gtctgcaggc aaaatacttc
    15601 ggccgcgaat tgtccattgg ccgcggcggt ttcggcggcc gacaacatca tggcgatcgc
    15661 ctcggtgagc gcgccctgcc cggccaccag ccatgcgcgg gccaggctgc gttcgtagtt
    15721 cagcgcccga aatggacgtc ggatttcatc aagcccggcg agcactgcgg ccgcgtcgtc
    15781 ggtcgaacca cgcatcgcca gcgccgtggc gtacgggatg tgataccgga atccccaccc
    15841 cttggcgttc cccgacgccg acaacgcggg cgctgccttt cccaacaacg cacacgccga
    15901 atccatacga cccgcgaaga gcgcagcgcg acccgcgact gcggcgccga gcaattgggc
    15961 tgcgccgggc atgtcggcgc cttgctcgta cacgcgttgt gccacgttga atccctcggc
    16021 catctggccg gccagcaaca atgcgctgat gtgcccgtcg gcgatgttga atcgcatgtg
    16081 cggggaatcg aaggcccgag atgcgacggc gtatcccgtg tttgcgacgg acaccgcctt
    16141 ggcggtggca cccgcgtccg cgtgcaccac ggtaagcgcc cacgcggtct cggcgcccgc
    16201 gaccgccggc aagtcttcca gcacaaggct tttcgatgtc tctattacct gttgcggctg
    16261 atccaccgca aaccagtacg cggtgaggaa ggcgtctatc cagatgcgcg ccgccggcgc
    16321 ggcgctacgg cccgcgccat cgatgatctc tttagcccgc gcggcatcac cgattgccca
    16381 cagcatgttg ctggcccgca ggtacgcgag ccggccgcgg tcctcgtcgg tgagctctga
    16441 aataggcagc ccggcgagca ccgcatccgc ctcctggccg gcaaacagcc acgacaacgc
    16501 atgtgcgcga aggaaatacg cgcgcggcga gccgtcagcg cgaattgccg ccttggcgag
    16561 ccgatccgcc agtcgtagat cgcccagcca gaccgcgccg ctgcccgcct tgaggagtag
    16621 atcggtatcg ggggtcaggt cggagtccag actcagcgag gcgcggcgca ccacgctgcg
    16681 catgtcgtcg gcgtctgcgg ccgacgcaag ttcggcggcg atcagaccgc gcagccggcg
    16741 caacctggtt tcgggtgatc gagatcggcg cacctcgccg tacaacggat gcgccagtcg
    16801 cacttccagt tcgtcgtcga cttcatcgac ggttatcagg ccgcgcaggt gggcttcctc
    16861 gatggcgtcg ggatccgcga tccgccgcag cgtcgtcatt acgagcggtt cgccgaccgc
    16921 aagtgcgtcg atcacctcaa tggtgggcgg cgacagcgcg ccaaaccggg cctcgatcaa
    16981 ttcgaccaat cccatcggca tgatcggttc gccgatccac tgccaatagc ggttctgctg
    17041 acgtatccgg ccgtcggcga tttcctgctc gacgatgttg cgcagataca aggcattccc
    17101 gcgcgtcagc ttccacagcc gggtggccgc atcggcatcc accggcccac ccagcgccgc
    17161 agccaacagc tcggcgatct cgtctttcga aaggggttgc aggtcgagtc ggccgaactg
    17221 ggcgcccctc cagatctcgt agatctcggt gggaacgcgt tcgccttcac gaacggtgag
    17281 caccaccttg gcggcctcgc gcagcacgat ctggtgcaag acgaaggtcg atagttcgtc
    17341 gagcagatgc acatcgtcga cgcccagcac caccttcgta ccgggcggcg cggatgtgac
    17401 ggcgtcgatc acgccgcgca ccagctggag tcggtcggtg gccgccgacc cggcccatgg
    17461 cgcgaacgca ccgaggggaa gcttgcgcgc ggacatggtg ccgacggccc agcgtggttc
    17521 gaaaccgtgg gatgcggccg acgacagccc ctcgcggacg atccggctct tcccgacgcc
    17581 cgcggccccg ctcaccagga tccccgcgac gccgggatcc gagattacgg ccgctatcgt
    17641 gcgcatctcc tcggagcgac cgatcaatgg ccaggtcaac cgcacatcga gagcctagaa
    17701 cgcttacacg gtttgccagg ggagattcgg ctagccatgt caacgtagcc gatcggctac
    17761 tatgctggcg tgacttccac agacgcgcag cggcttggag acatcccgcg caccgcggcg
    17821 gagacccgcg tcatgttggc cgcgcttgat ctgttcgccg aacacggcgt cagcggtacg
    17881 tcgttgcaga tgatcgccga cgcgatgggc gtgacgaaag ccgctgtgta ccaccagttc
    17941 aagaccaagg acgagatcat catcgcggtc accgagatgc agctcgcccg cttggaggcg
    18001 gctctggagg acgccgaggc ggccgacgat cggccgcagg caagggaatt gctgttgaat
    18061 cgggtgatcg agcttgccgt cgagcagcgc cacatggtgg gagtcatgca atccgatccc
    18121 gtcatcattc gattcctcgc ggagcacaag ccgtttcagg ccttcctcga gcgcctgtac
    18181 gccgcgctta ttggggacga cgcgggtccg gagacgttgg tgcaggcggc gatgctgtcc
    18241 ggagcgattg gcagcgccgt caagcatcct cttgtcgcac acctcgacga cccgacgctg
    18301 cggtcgcacc tgctgcgcct gacacggcgg ctggtcgacc taccccgctg agtaggcgat
    18361 tccgattgct ttctattcag gaagtcagtc agctaagccg tgccagacga tggggtcgct
    18421 cctggcgtgg gcggcccctt catctgcccg gccgagtcga ggtcgcgcag cgccggcagg
    18481 aagatcgcga ctaggccaag tgccagcatc ggcagcgaca acgcaaggaa cgtcgcatgc
    18541 agtccggcgg agtcggccaa cgggccggcc acgatcagtc cgagcggccc cgcggcatag
    18601 gccaacgacc ccattacgcc caccacgcgg ccccgaagat gctgcggcgc acgggtttgc
    18661 atgacgtagt tgtagatcgg cgcgatcggc ccgtagacca gcccgacgac ggcgcacagc
    18721 acgaggatga cgggcagcgg cggcaggaag gcgatgatag tcatcgccac ccccaacgtc
    18781 agcacggccg tcagcatgac tgcccgtcgg ctcgagtact tcgacagcac cgcatagcca
    18841 agcgctccca ccagcccgcc gacgcttaac gccatcagca cccagccgag ctgcgccggt
    18901 tcattgcgat cggtgaagta cttcggaaac agcacggatt ccatcggcat gtacaggccg
    18961 gtggcggtca ggtcgacgat ggccagcgtc cgcagcacct tgttgttcca cacgaagcgc
    19021 aagccttcga cgatgcccgc ccacactcct tcgggcagcg ccgcccggtc cggtttgccc
    19081 gcgccctcca gccgaagcgc cccgatggca acgatcgaca gcccgaacgc ggaggcggtc
    19141 acccacatgg tgttgacgcc gccgagcgtg gcgatcagca ggccgccgat gccggggccg
    19201 acgatgtacg ccaggttgaa gaccgcctcg tagacgctgt tggcgtggtc gagcgtccag
    19261 ccggcgcggg tggccgcctc cggcagcatc gtctcgcgtg cggtcatccc cgccgggtcg
    19321 aagaacgcgc caagcgcagc cagcgcggca agcaccgcga cgttgatgac gtgcaccccg
    19381 aacatcagtg ccaggacagg gacggcggcc accgacagtg ccgacaatgc gtcggaaatc
    19441 atcgaaaccc gcctgcggcc gaggtagtcc accgcggcac ccgcgatcag cgtcgcggcg
    19501 agcaacggca gggtgcccgc catcgccacg atcgaagcgt tcagcgcaga accgttgcgt
    19561 tgcaacacaa gccagggaaa cgcgacgatc gagatgccgt tgcccgcgcc agccatcagc
    19621 gcgccgaaca gaatcaggaa aagcggaccg cgcttgccgt tcgtcataag ttatcgcgcc
    19681 gaatctaaca gcggtcgagg ccgctgggca ggtggttttg ctgcgcatgc ttcagtgatt
    19741 ctgtgaaact cgcccatcca cggaccggcg tgatgttcga ttcgcccgtg ctccccggct
    19801 ccggctggcc gggcgatccg gccacctcgc gaacggccgt ggcggaaacg ccagctcacg
    19861 tcgcctcgat ggcggcggcg gtcaggactc ttgaggagct cgacgccgag gtgtcggtgt
    19921 gccgtgcctg cccgcggctg gtcgaatggc gtgagcaggt ggccacggac aagcgcaagt
    19981 ccttcgccga tcagccgtat tggggtcgac cgattccggg ttgggggtcg gcgcggccgc
    20041 gggtgttgat cgtcgggttg gcgcccgccg cgcacggcgg taaccgcacc ggccgggtct
    20101 tcaccggtga ccggtcgggc gactttctgt tcgcggcact gcatcgcgcc cggctggcca
    20161 accaagcgac gtgtgtggac gcggcagatg gtttggtgct caacgacact cgggtagctg
    20221 cggccgtgcg atgtgcgccg ccggccaatg tgccgacacc ggccgaacgc gcggcgtgtg
    20281 caccgtggct ggatgccgag tggcggctga tcggatccga ggtgcaggtg atcgtcgcgc
    20341 tcggggcgtt cgcgtggcgc gcggcgctgc agatgatcgg ctccgtcccg acgccggcgc
    20401 cgaagttcgg acacggcgcg acggcgacgc ttggtgacgt gacgctgatc ggctgttatc
    20461 accccagcca gcagaacacg ttcaccggca agctcacacc gcagatgctc gacgacatct
    20521 tcagtaaggc ggccgaagtg cactgagagc gatggccacc cgacatcatt cgaagaccgc
    20581 cacgactatc aggacggttg tagccatatc gcggcactag gcagatctcg tgacctcgtc
    20641 gagcgcgtgt gccaggagat cgacgctcga catcgcgctc aatccaatca cttccgactg
    20701 ctccgcggtc agtggcagct ggtcgagcgt ggcgaaaatt tcatcgccgt ggccgatgtc
    20761 gagttcggcg tgcgcactta gcgtgcggaa tgcctgcagt tcgtaacccg tgcgggccat
    20821 cagctcgtca atcgtcgcag gtgttggtgg atagccctcc aaaagcccga tccacccgag
    20881 cagcgcgaca gggtggtaat gcaagaccca gtaatactgc gcgcccaccg cgcgcgcgac
    20941 cgtcggcgac ggcacgcgcg cggtgatggt tgagcggtcg acgccgagcg atgcgagatc
    21001 ctcgagtagc cactcgtcgt ggtcgcgctc ctcttcgatg tgctcgtcga ggtactccac
    21061 gagacgggcg gcgaccgggt cggcagccga aatcgagcgt gctcggtcac gtgctgtctc
    21121 catgagtggc acgctcgcgc ggattaccga atgaagggtg aacaggtact cgggataaag
    21181 ctcgcgcacc cggggatgct taaccaaccg ctcgccggcc gccgagagct tccacagtac
    21241 gagttccagc gtcccgcgga gccgctcagc gttgcttagc ggcgacgtcg aggcgaccgg
    21301 ccgcatagcg agcatcgact ttagttggtc tggctagtgg gccatcaacg gatactgcgg
    21361 caaggatagg ccggcactga cgagcgtggt gttgatgtcg gacagcaggg cgagctcaga
    21421 gtccgacagc gtttcgaaca actgacgcac cggttcgggg agctgactgt agtcggcgtc
    21481 ccccaagtct cctggccggt gccgattgaa caccgcttcc ttttcaccgc ggggggccgc
    21541 tagccactcc tgccggccct cagcgtcctt gatgatcgac tcgaacacaa caagcgcttt
    21601 cagctgtgcc tcgttcactc cgacgtcggg gctcgtcatg gcgactcctg atttggtgtt
    21661 atcccacctc accctgacta aggttccttg tcaagacggg gaaagatgtc ccgtggaagg
    21721 gggccttgac gtggctgccg acattcgcaa agtcgtcacg gtccttttct gcgatatcac
    21781 cggctcgacg accctcggtg aagccctcga cccagagtcg ctgcagcgaa tactgtcccg
    21841 atacttcggt gagatgaagg cgatcgtgga acgccacgaa ggcgccgtgg agaagttcat
    21901 cggtgatgcc gtgatggcgg tcttcggcct cccgtcggtg catgaagacg acgcgctgcg
    21961 tgccgttcgc gcggcggttg agatgcggga cgcactcgct acgctcaatg ctgagttcgc
    22021 cgtgaaatgg ggtgtcacac ttgccatccg caccggcatc aacactggcg aaattcttgc
    22081 gggagagccg ggtgaggggc aagcacttgt cgtcggcgac gccgtcaaca ccgcggcccg
    22141 tctcgagcaa ttcgccgagc cgggtcaaat cctcatgggg gaaagcacct actggcttgt
    22201 tcacgatgca gtgacagccg aacccgtcgg tcctctgggg ctcaagggca agagctcggc
    22261 ggtatccgct tggcagcttt ttgccgtcgt cgcccacgcc ccaggctgga gtcggcgact
    22321 tgactctcca atcatcggac gcgaacagga gatccgccga ctggagaacg cctttgaacg
    22381 cacggtggaa aacggcgcat gtgagctcat taccgtcatg gctcccgccg gcgtcggcaa
    22441 gtcgcgactg atcgcggaag tgctgtcgcg cttggtatca cgcgccacca tcctgcaagg
    22501 gcgctgtctc ccatacggtg acggaatcac gttctggccg atcgcctccc tcctgatgga
    22561 ggcgatcgaa atgaccgagc gagacacgga gatgaccgcc cggcgcaggc tgtccgagct
    22621 tcttggccag accgcggacg accaagagga tgccgagctt gtgtgtgacg ctctcgcgcc
    22681 gttgcttggt ttcggcacgg aggcggtggg gatccgtgaa accttctggg cggtccgaag
    22741 gctgctcgag cacctcgccg cccggcggcc gattgtcgtg gtcttcgacg atatccattg
    22801 ggggcagccc acattcctcg acctcgtcga gtatctcacc gactggatcc ggggcgtccc
    22861 tgtactcctc gtgtgccagg cgcgacctga gctgcttgac acacgtcctc gatgggtgtc
    22921 gacgaagagc aacgcgtcgg tgatgactct gcagccgctc accgaagcac agaccaaaac
    22981 cctgatccgt ggactcaccg gcggcgcgga tcttccattg gaggcgcgaa cgaggatcgc
    23041 agctgtggcc gagggaaacc cgctcttcgt cgaggagacg gtgcggatgc ttgtcgacca
    23101 cgccgtcctg caatcgcgtg acgggcgctg gttagtcagc ggcgatctgt cggcaatcgc
    23161 catcccgccg accatccacg cgctgctcgc ggcacggctt gatcggctgg agccggacca
    23221 gcggatggtt atcgagcaag catctgtcat cggtcgttcg ttctggtggg gtgcggtgtc
    23281 tgagctggcc cccaaagaga ttcagcagca tgtcggggag tacctccagt cgctcgtgcg
    23341 cagagagctt attgaaccgg acgagtccga gatccgcggc gaggacgcct tccgcttcac
    23401 ccacatcctc atccgcgacg cggcttatca cggcgttccc aaggcaacgc gtgccgagtt
    23461 gcatgagcgt ctagccggct ggatcgcggc tcacacgcgg gaactggctg gtaagtacga
    23521 aacgatcgtt ggttaccacc tcgagcaggc gcatcatgcg ctcatcgagc tcggtccgca
    23581 gagcgatcgc tcttcggaac tggccaatcg ggcggcctcg acactggcgg cggccggcga
    23641 tcgtgcgctg gcccgcggcg acatgccggc ggcggtaaac ctactgtcgc gagccgccgc
    23701 gttgctgtcc ccgcaggacg ggcgacgact ggaactgctt accgaagtgg cgttcgcgct
    23761 catcgagatt ggtgagtttg accgtgtggt cggagtcgtc gaggagttga acactgcagc
    23821 gaaagagacg aacgatgcgg gcctgcagtg cgaagcaacc attgtcggtc tgtggatccg
    23881 gttgttcacg aaccccgatg ggtgggcagc cgatgctacg aaacaggcga agcgagcgat
    23941 caccacgttc ggtgagctcg aggacgatgc tggcctggcg agagcttggt cgctgctggg
    24001 cctgacgcaa atgatgacag cgcgctttgc agcagctgag gactcgtggt ggagagccgt
    24061 tgggcacgcg cagcgagccg gcagtcgccg ggatgtgctc gagagcctct cgtggattcc
    24121 tggcgcacta tgggcggggc ctacgcccgc ggtagagggc atcgcgcgct gccgcgatgt
    24181 gctcgaacag gcccgcggcg ataagaaggc aatggccagc gcgctgttct cgcagggctt
    24241 cctcgaagga tgtgtcgggc aattcgacga cgcacggcag cttttcgcgc aggcgcgcgc
    24301 gctcctcgaa gaggttgcgc tgccggtctg gatcgccggc ccattgacgc agtgcgtcgg
    24361 gtggaccgag ctgttggcgg gcaacgcgca aggcgcagaa cgcgaactgc gcgcgggctg
    24421 ggaaacgctg caggcgatcg gcgaggtgac gttgctctcg acagtcgccg ggctgctggc
    24481 cgaagcggta tatgcgcaag agcggtacga cgaggccgaa cacttcaccc gcctgagcga
    24541 ggagtccgct ggccccgaag acgtttactc ccacgccttg tggcgcagtg tgcgatccaa
    24601 ggtcctcgcc tggcgtggcg agttggatga agcactgttg cttgggcgcg aggcggttgg
    24661 ttttgccgaa tccagcgact caccccttct tcgctggcac acgctggtga gccgagcagc
    24721 ggttcttcgt atcgccaatc aaccgcaagg agcagaggag gatctgcgcg aggcggccga
    24781 cctcgccgag cagaaaggca atgtcgttgc cgcacgcatt gcgcgggaag cgctcggtcg
    24841 cggggcctgt taggcggggc aagcgtgagt agggaagccg caggggtggg gaggcgttga
    24901 ggtagccatg cgcctttccg ttctcgacct cgtgccggtg cgcaccgacc agaccacgtc
    24961 cgacgcgctg gcggcgacga cgcagctcgc gcagacggcc gaccggctcg gctacacccg
    25021 gtactggctg gccgagcacc acaacatgcc tgcggtggcg gccaccagcc cgccggtgtt
    25081 gattgcgcat ctggctgcgc agacgtcgca gctgcggctg ggttcgggtg gggtgatgct
    25141 gcccaaccac gcgcccctgg cggtcgccga gcagttcgcg ctgctggagg ccgcgcatcc
    25201 cggccgcatc gacctcggca tcgggcgcgc acccggctcg gacccggtca cgtcgctggc
    25261 gctgcgcggt gcggcgggcc gcgatgatcg cgacatcgag gccttcccgg agtatctcga
    25321 cgacgtcgtc gcgttgatga gcgcacgcgg cgtgcgggtg ccgattccga accagcgcta
    25381 catcctcaag gcgacgccgg ccgcggccac cgagccgaag ctgtggctcc ttggctcgtc
    25441 gatgtattcg gcacatctgg ccgcggcgaa aggtctgccg tacgtgttcg cgcaccactt
    25501 ttcggggcag ggcacggccg aggcgctcga ggtgtatcgc tcggagttcc ggccgggcga
    25561 gctggcgtcc gaaccggtga cgttcctgac ggtcaacgcc gtggtggccg aaacacgtga
    25621 tgaggcaatg gctttgatcc tgccgaacct gcagatgatg ggacggctgc ggacggggca
    25681 gccactgggc ccgctcgatc ttgtcgagga cgccgcggag gtgacgatgg cgccgcaggc
    25741 ggagcggatc gcggagggtg cgttgcgacg cgcggtcgtc ggcaccccta cggaggcggc
    25801 tgagcaggta cgcgccctgg ccgacgagtt cggcgtcgac gaagtgatgg tcaaccccgt
    25861 cgcctcggcg tatcgcggca ctgatccggc ttccgcgccc gcgcgcgaca agacgttgga
    25921 actgctcgcc aaggaactgt tctagtttcg ccgagcgcac ggttgttgta cgcatcggcc
    25981 gagaagatcc gcgaacaacc gtgcgctcgg cgcactaagc gggcgtcagc accaccacag
    26041 gtatcggccg cgacgtgcgc ttctgatagc cgatgtagcg gttcttgttg ccccgcatgt
    26101 tgttgacgat cggccacagc cgctgatagt cgggatcgtc tggcagcacc ggtttcgcgg
    26161 tgacgccgaa tcgctttggg cccacattga tctcgacctt ggggttggcc ttcaggttgt
    26221 gataccagcc cggtgccttg gggtcgccac ccttcgacgc gacgaccagg tagtcgtcac
    26281 cgtctcgggc gtagctgagt gaattggcgc gcttctggcc ggttttggcg cccgtggtgt
    26341 ggaggatcag ttgagatggg ccgcccggga tcgtgtgccc gatccggcca ttggtgccct
    26401 tatag
//
LOCUS       DASRJA010000584        11673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2852902,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000584 DASRJA010000000
VERSION     DASRJA010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11673
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2852902"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /gene="purM"
                     /locus_tag="VFC01_25930"
     CDS             <1..883
                     /gene="purM"
                     /locus_tag="VFC01_25930"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZN83090.1"
                     /translation="SSTDGVGTKLAVAQAMDKHDTVGIDLVAMVVDDLVVCGAEPLFL
                     QDYIAVGRTVPERVSELVSGIANGCVMAGCALLGGETAEHPGLMAPDHYDISATGVGV
                     VEADDVLGPDRVKPGDVIIAMAATGLHSNGYSLARKVLLEIDRMNLAGHVEEFGRTLG
                     EELLEPTRIYAKDCLALAAETQVRTFCHVTGGGLAGNLERVIPPGLVAEIDRGTWTPA
                     PVFGMIAQRGRVERVEMEKTFNMGVGMVAVVAPDDTDRALAVLTARHLDCWTLGTIKK
                     GGKDSPRASLVGQHPRF"
     gene            complement(888..1073)
                     /locus_tag="VFC01_25935"
     CDS             complement(888..1073)
                     /locus_tag="VFC01_25935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HZN83091.1"
                     /translation="MGRGRAKAKQTKVARELKYSSPQTDFDRLQRELAGSDGSDDLNG
                     TDAVTDDWAPEEDDWRR"
     gene            complement(1197..2270)
                     /locus_tag="VFC01_25940"
     CDS             complement(1197..2270)
                     /locus_tag="VFC01_25940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HZN83092.1"
                     /translation="MSAVSAPETGPDAGAVWHYGDPLGEQRAAADGAVVVDRSHLVVL
                     MLTGKDRKTWLHSISSQHVSDLPDGAVTQNLSLDGQGRVEDHWIQTQLDGVTYLDTEA
                     WRGEPLLTYLRKMVFWADASVEPADLAVLSLLGPKLADPQVVDVLGIGSLPAEDAAVP
                     LPGGGFLRRMAGQTIELDLVVPREQAAAWRQRLVDAGVRPAGVWAYEAHRVAALRPRL
                     GVDTDERTIPHEVGWIGTAVHLDKGCYRGQETVARVHNLGKPPRMLVLVHLDGAADRP
                     ATGDPVLAGGRTVGRLGTVVDHVDEGPIALALLKRGLPADTPLTTGGQTEVAAVIDPD
                     SMPPTDAVGAGRLAVEQLRGRAR"
     gene            2305..3192
                     /locus_tag="VFC01_25945"
     CDS             2305..3192
                     /locus_tag="VFC01_25945"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HZN83093.1"
                     /translation="MASEPAVVVTLDGEQHDPAAPLLHADDLAVVRGDGIFESLLVRD
                     GRPCLLEAHLGRLTQSARMTDLPAPDLRRWREAAATAASSWTAKSDDEGVMRLIYSRG
                     REGGSEPTGFVLVGPLPARVAAVRRDGLAALTLARGLPADGTADMPWLLAGAKTLSYA
                     VNMAALRHAERHGAGDVIFVSSDGCILEGPRSTVVIATESENGAGNSTLFTPPPWYPI
                     LRGTTQQALFEVARNKGYDCDYRALRPADLFAAQGVWLVSSITLAARVHTLDGRVLPP
                     SPLASELTELVDAAIVSDR"
     gene            3225..4127
                     /locus_tag="VFC01_25950"
     CDS             3225..4127
                     /locus_tag="VFC01_25950"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN83094.1"
                     /translation="MGDDSGDERLTRYQRLALRRLMRHAGLRPGQPLTSQDWQAYFEF
                     STVRSTRAVHRVMRALPSSPRCGMCGAPFAGFGSRLVRPLGFRPSRKNPTLCATCVEL
                     APPGGITTEVGVMFADLRGFTTRSESITPQEATALLRRLYAVAEDVLFPEALIDKLIG
                     DEVMALYLPIFVAGSSFTPEDADRRTVAKVMLDHARRLLEGLGYGSREGPALELGIGL
                     DYGEAFVGNIGGKGAISDFTAIGDVVNTAARLQSCASSGEVLVAERLARYLERPAGPV
                     EHLAVKGKHEPVAAQRVCWFPKHS"
     gene            complement(4351..4707)
                     /locus_tag="VFC01_25955"
     CDS             complement(4351..4707)
                     /locus_tag="VFC01_25955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83095.1"
                     /translation="MTGKAVRFAVVGAAAAALISTAACSSSTTPAEQTSAAPSSSAHD
                     AFAQCLSDNGVPAPPGDGHAGGPPPNGTPPSGPPPSGAAPSSNGTPPAPPGVDQATWD
                     KAMQACSSLAPAPPEK"
     gene            4841..5680
                     /locus_tag="VFC01_25960"
     CDS             4841..5680
                     /locus_tag="VFC01_25960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN83096.1"
                     /translation="MRNVWRLAALVAAMATACAPIPDKATTASADKCTKDALGTLYPG
                     IFTFGADQPVYPPWYMGDDPASGEGFEAALAYAIAAKMNYTRDDVRWVRVPFNAALAP
                     GPKTFDANLSEFSITEQRKGAVDFSSPYFDVTQAVVTVKSSPAAGVKRVHQLKKLRLG
                     VQVGTTSYTAATSVDGEVPVEVYNTNGDAKLALSNGEIDALVADLPTAFTVANELRDG
                     LMVGQLPSASGDVEQFGVVLDKGSGLTRCVSWAVDSLRAEGALDRLEERWLTDAGKAP
                     VLA"
     gene            complement(5677..5934)
                     /locus_tag="VFC01_25965"
     CDS             complement(5677..5934)
                     /locus_tag="VFC01_25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83097.1"
                     /translation="MRAIALAAVVAAAGILAACSSNTPSPEQVSTSVAPSGHGGFAHC
                     LSEHGVPIAPGPAVGPPPGVDQDIWHRAMQACSSLAPGPAS"
     gene            complement(5934..6563)
                     /locus_tag="VFC01_25970"
     CDS             complement(5934..6563)
                     /locus_tag="VFC01_25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HZN83098.1"
                     /translation="MTSGDEAVAAAAERAKATAARNIPVFDDLPLPADTANLREGANL
                     NDALLALLPLVGVWRGEGEGRGPGGDYRFGQQIVVSHDGRDYLIWEARSWRLTETGEY
                     DEPTLRESGFWRFVDDPADPSESQAIELLLAHSAGYVELFYGRPRTQSSWELVTDALA
                     RSKSGVLVGGAKRLYGIVEGGDLAYVEERVDADGGLVPHLSARLSRFVG"
     gene            complement(6716..7036)
                     /locus_tag="VFC01_25975"
     CDS             complement(6716..7036)
                     /locus_tag="VFC01_25975"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN83099.1"
                     /translation="MRIAIAGFATTVFAFITVATAPFAAATPDQEFLDALANGGVTFA
                     SKATQQVITHGHMVCQDFASGAAFTDAVGGVAGGMGGNQRLAGQFVRAATNTLCPKYV
                     DELP"
     gene            complement(7152..7454)
                     /locus_tag="VFC01_25980"
     CDS             complement(7152..7454)
                     /locus_tag="VFC01_25980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1416 domain-containing protein"
                     /protein_id="HZN83100.1"
                     /translation="MCSAPKQGLTLPASVDLEKETVITGRVVDGAGQAVGGAFVRLLD
                     SSDEFTAEVVASATGDFRFFAAPGTWTLRALSPAGNGDASVAPSGAGIHEVDVKVA"
     gene            complement(7457..8290)
                     /locus_tag="VFC01_25985"
     CDS             complement(7457..8290)
                     /locus_tag="VFC01_25985"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HZN83101.1"
                     /translation="MARSDVLVSTDWAESNLTAPNTVFVEVDEDTSAYDGGHIEGAIK
                     LDWKEDLQDHVKRDFVDAQQFSKLLSERGIANDDTVILYGGNNNWFAAYAYWYFKLYG
                     HDKVKLLDGGRKKWELDGRPLSTDAVNRPETSYTAKDPNNAIRAFRDEVIAAISKKNL
                     VDVRSPDEFSGKILAPAHLPQEQSQRAGHIPGALNVPWSKAANEDGTFKSDEDLATLY
                     AEAGLDGEKETIAYCRIGERSSHTWFVLQELLGHKNVKNYDGSWTEYGSLVGVPIELG
                     S"
     gene            complement(8326..8796)
                     /locus_tag="VFC01_25990"
     CDS             complement(8326..8796)
                     /locus_tag="VFC01_25990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HZN83102.1"
                     /translation="MSTNTKTAAPALVDVRGPRFAAWVSTVVIVATLIVSAFSSSSAA
                     VILALQALVFAIGAWRGPRQHPYGLIFQSLVAPRLGPVTEKEPVPPLKFAQLVGFVFA
                     VVGTIGFAAGVPLLGLVATGFALVAAFLNAAFGICLGCQVYPLVARFRRAPSPA"
     gene            complement(9013..9435)
                     /locus_tag="VFC01_25995"
     CDS             complement(9013..9435)
                     /locus_tag="VFC01_25995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZN83103.1"
                     /translation="MSSTWVLVITVLVAALGVAYVIGKLLTLRAGMLKSAEEASNVDT
                     SDLGLSSSGPTVLHFSADWCGPCAGVRRVVDQVCDELPGVAHVEIDMDANPQAARRLS
                     VMSLPTTIIFDADGRPRYRTTGVPRAADLRSALEPLLA"
     gene            complement(9432..10247)
                     /gene="lmeA"
                     /locus_tag="VFC01_26000"
     CDS             complement(9432..10247)
                     /gene="lmeA"
                     /locus_tag="VFC01_26000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannan chain length control protein LmeA"
                     /protein_id="HZN83104.1"
                     /translation="MGQNVRVRKLLIGSIATVTAVAVGAVGADFGAAIYAEYHLARSV
                     RIATNLNFDPWVSILGFPFVTQATRDHYNEVEIRASGVDHPVVGKVGLEATLHSIDLT
                     QTSWLIGPDAKLPVGELESRIIIDSTHVGRFMGIPDLLVEAPTRESNDATGGTTESGI
                     SSNRGLVFTGTPNKADYDEKVSIAVDLSVAGADQTTLVFTATGVLTGAGTADQQVPED
                     KLPAVLAAFSISMPGQKLPFGVRPTSEGARGSDIIIEGITKGVTIPLNGFRLS"
     gene            10406..11188
                     /locus_tag="VFC01_26005"
     CDS             10406..11188
                     /locus_tag="VFC01_26005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN83105.1"
                     /translation="MDLLLLTVDPHPESVLPSLSLLAHNVRTAPTEVSSLLEAGTADV
                     AIVDARTDLAAARGLCRLLGTTGTSVPVVAVVNEGGLVAVNVEWGLDEILLPSTGPAE
                     IDARLRLLVGRRGGVANQENVGKISLGELVIDEGTYTARLRGRPLDLTYKEFELLKYL
                     AQHAGRVFTRAQLLQEVWGYDFFGGTRTVDVHVRRLRAKLGPEYESLIGTVRNVGYKA
                     VRPARGRPSTPGSELSDDFVDDVGSYEAPDGLPDALVDPLRQ"
     gene            11185..>11673
                     /gene="mshD"
                     /locus_tag="VFC01_26010"
     CDS             11185..>11673
                     /gene="mshD"
                     /locus_tag="VFC01_26010"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HZN83106.1"
                     /translation="MTDIGWRAGLSDDEQHRIRELIAAAEQADGIAPVGDQVLRELPH
                     DRTRHLLAVDGAEVIGYLNLTEQPAMAELVVHPDTRRRGVGSAMARIGLSEGGDRARI
                     WAHGNLEPARATAAALGLVADRELLQMRRPLTGLPPASVPDGVRITTYSGPADDAELL
                     RVN"
BASE COUNT         1919 a         3816 c         3986 g         1952 t
ORIGIN      
        1 gtcgtccaca gacggcgtcg gcaccaagct ggccgtcgcc caggcgatgg acaagcacga
       61 caccgtcggc atcgacctcg tcgcgatggt cgtcgacgat ctcgtggtgt gcggcgccga
      121 gccgttgttc ctgcaggact acatcgcggt cggccgaacg gtgccggaac gcgtcagcga
      181 actggtgtca ggcatcgcca acggctgcgt gatggccggc tgtgcgctgc tgggtggcga
      241 gaccgccgag cacccgggcc tgatggcgcc cgaccactac gacatctccg cgaccggcgt
      301 cggcgtcgtg gaggccgacg acgtgctggg gccggaccgg gtcaagcccg gtgacgtgat
      361 catcgcgatg gcggcgacgg gcctgcactc caatggctac tcgctggccc gcaaggtgct
      421 gctggaaatc gaccggatga acctggcggg ccacgtcgag gagttcggcc gcaccctggg
      481 cgaagagctg ctcgagccca cccgcatcta cgccaaggac tgcttggcgc tggccgccga
      541 aacccaggtc cgtaccttct gccacgtcac cggcggcgga ctggccggaa acctcgagcg
      601 ggtcatcccg cccgggctcg tcgccgagat cgaccgcgga acgtggacgc ccgccccggt
      661 gttcgggatg atcgcgcaac gcggccgcgt cgagcgcgtc gagatggaga agacgttcaa
      721 tatgggtgtc ggcatggtcg cggtcgtcgc acccgacgac accgaccgcg cactggccgt
      781 gctgacggcc cggcacctgg actgctggac cctcggcacc atcaagaagg gcggcaagga
      841 cagtccccgt gccagtctcg taggccagca ccccaggttc tagggatcta gcgccgccag
      901 tcgtcctctt cgggggccca gtcgtcggtc accgcatcgg tgccgttgag atcgtcagaa
      961 ccgtctgacc ccgccagctc gcgctgaagc cggtcgaaat cggtctgcgg tgagctgtat
     1021 ttgagctcac gagcaacctt ggtctgcttt gccttagccc ggccgcggcc catgggggta
     1081 ccccctcgcg caataacgga gcggcccaat atttaggcgg ctccgatctg tgtgtcttta
     1141 ttgtcctgcc aacactttac cgtgcccggc tgtcgtgtgc tggcaggccc atcgcgtcaa
     1201 cgcgctcggc ctcgaagctg ttccactgcc aaccggcctg cgccgaccgc gtcggttggt
     1261 ggcatcgagt cggggtcgat gaccgcggcg acctctgtct gcccgccggt ggtcagcggc
     1321 gtgtccgcgg gcagaccgcg cttcagcagg gccagcgcga tcgggccctc gtcgacgtgg
     1381 tcgaccacgg tgcccagccg gcccactgtg cgtccgcccg ccagcacggg atcgccggtg
     1441 gcgggccgat ccgcggcgcc gtccaagtgc accagcacca acatccgggg aggtttgccc
     1501 aggttatgga cgcgagcgac ggtttcctgc ccgcggtagc agcccttgtc caggtgcact
     1561 gccgtaccga tccagccgac ctcgtgcggg atggtccgct cgtcggtgtc gacgcctagg
     1621 cggggccgca gcgctgccac ccgatgggct tcgtaggccc acacaccggc cggccgcacg
     1681 cctgcgtcga cgaggcgctg gcgccacgca gcggcctgtt cgcgcggcac caccaaatcc
     1741 agttctatgg tctggccagc cattctgcgc aaaaacccgc ctccaggtag cggcacggct
     1801 gcatcttcag ccggtagcga accgatgcca agcacgtcga cgacctgcgg gtcggccagc
     1861 ttcgggccga gcagcgacaa caccgccaaa tcagccggtt cgacggacgc gtcggcccag
     1921 aacaccatct tgcgcaggta cgtcagcagc ggttcgccac gccaggcctc ggtgtccagg
     1981 taggtgacgc catcgagctg cgtctggatc cagtgatctt cgacacggcc ctgcccgtca
     2041 aggctgagat tctgcgtgac cgcgccgtcg ggcaggtcgc tgacgtgttg cgacgagatg
     2101 ctgtgcagcc acgtcttgcg gtccttgccg gtgagcatca gcaccacgag gtgtgagcgg
     2161 tcgacgacga cggcgccatc cgcggctgca cgttgctcgc caagagggtc gccatagtgc
     2221 cagacggcac cggcatccgg tccggtctcg ggggcggaaa ctgcggacat gtcttcaacc
     2281 ttacgggcgc tacgcttctt gcccatggcg agcgaaccag cggtcgtggt caccctcgac
     2341 ggtgaacagc atgaccccgc ggcgccgttg ctccacgccg atgacctggc ggtcgtgcgc
     2401 ggcgacggca tcttcgagtc gctgttggtc cgcgacggca ggccgtgcct gctagaggca
     2461 caccttggcc ggttgacgca gtcggcgcgg atgaccgacc tgccggcacc ggatctacgg
     2521 cgctggcgcg aggccgcagc cactgcggca tccagctgga ccgcgaagag cgatgacgag
     2581 ggcgtgatgc ggctgatcta cagccgcggc cgggaaggcg gttcggagcc gacgggcttc
     2641 gttctggtcg gcccgctgcc cgcgcgagtc gccgctgtgc gccgcgacgg gctggccgcg
     2701 ctgacgctcg cccgcgggtt gccagcggat ggcacggccg acatgccgtg gctgctggcg
     2761 ggcgcgaaga ccttgtccta tgcggtgaac atggcggcgc tgcggcacgc cgagcgccac
     2821 ggcgccggtg acgtgatctt cgtcagctcc gacggctgca tcctcgaggg gccgcgctcg
     2881 acggtggtga tcgccaccga atcagagaac ggggccggga attccacatt attcacgccg
     2941 ccgccgtggt atccgatcct gcgcggcacc acgcagcagg cgctgttcga ggtggcccgc
     3001 aacaagggct acgattgtga ctaccgcgcg ttgcgcccgg cggatctctt tgcagcgcaa
     3061 ggggtttggt tggtatccag catcacactc gcggcgcggg tgcacaccct cgacggtcga
     3121 gtgctgccgc cgtcgccgtt ggcgagtgaa ttgaccgaac tggtcgacgc cgcaatcgtg
     3181 tccgatcgct gaccgcgatc cggaaagtgc gccattctgg gtgagtgggg gacgattccg
     3241 gagacgaacg gctgacgcgt taccagcgtc tggcgttgcg ccgtctgatg cggcacgccg
     3301 ggctgcggcc cggccagccg ctgacgtccc aagactggca ggcttacttc gagttctcga
     3361 cagtcaggag cacgcgggcg gtacatcgcg tgatgcgagc actgccgtcg agcccgcggt
     3421 gcggcatgtg cggtgcgccg ttcgccggct tcggcagccg gttggttcgg ccgctgggct
     3481 tccgcccgtc gcggaagaat ccgacgctgt gcgcgacgtg tgtcgagctg gcgccgccgg
     3541 gcggcatcac gacagaggtc ggcgtcatgt tcgccgacct gcgcgggttc accacgcgtt
     3601 cggagtccat cacaccgcag gaggcgaccg cgctgttgcg acgcttgtat gcggtcgccg
     3661 aggacgtgct cttcccggaa gcgctgatcg acaagttgat cggcgacgag gtgatggcgc
     3721 tgtatctgcc catcttcgtc gctggaagct cgtttacgcc tgaggatgcg gaccggcgaa
     3781 cggtggcgaa agtcatgctc gaccacgccc gccggttgct cgaaggactc ggctacggca
     3841 gtcgcgaagg tcccgcgctc gaactcggca tcggcctcga ctacggcgaa gcgttcgtcg
     3901 gcaacatcgg cggaaaaggc gcgatcagtg acttcacggc cattggcgat gtcgtgaaca
     3961 ccgcggcacg cctgcagtcg tgcgccagca gcggagaggt actggtggct gaacgcctcg
     4021 ctcgatatct cgaaaggccg gccggtccgg ttgagcatct cgcggtcaag ggcaagcacg
     4081 aaccagtcgc cgcccaacgg gtgtgctggt tcccgaaaca ctcctaaatc ggttgtcgcc
     4141 gtgcggatgc gcaggtactt tcgttcatac acaaggaagg aggtggtccg accaattgat
     4201 tgacttacgg acatgtgagg tggctgcgag ctagcagcgc acggggagtc acctgcgcgc
     4261 gctggcgaat tccccgcagc cacccggccc ccgagccctc ggtcttgtcc aaccaggaaa
     4321 catcggctcg ggggccgctc catatctggg ctacttctcc ggcggagccg gggccaacga
     4381 cgagcacgcc tgcatggcct tgtcccacgt ggcctggtcg actccgggcg gagcgggggg
     4441 tgtgccgttc gacgatggcg ccgcaccgga cggcggcggt ccggacggcg gggtgccatt
     4501 gggcggcggg ccgcctgcat ggccatcgcc gggcggcgcc ggaactccgt tgtcgctcaa
     4561 gcattgagcg aacgcgtcgt gggcggacga ggatggcgcg gcgctggtct gttccgcggg
     4621 cgtcgtgctg gacgaacacg ctgcggtact gattaatgcg gcggcggccg cgccgacaac
     4681 cgcgaagcga acggctttac ccgtcacaat ttctccttgt cgagtgaacg agcgtctgcg
     4741 acggtagggg ccccggatgg gccgtcgtta ttagcgagct atgcgccagc tatggaccga
     4801 accctggcgc tggttagcta ggtgcgcgat gatgcgccac atgcgcaacg tgtggaggct
     4861 ggccgcgttg gttgctgcga tggccaccgc atgcgcgccg atccccgaca aggccacgac
     4921 cgcctctgct gacaagtgca ccaaggacgc gctgggcacg ctgtatcccg gcatcttcac
     4981 gttcggcgcc gaccaacccg tatacccgcc gtggtacatg ggcgacgatc cggccagcgg
     5041 tgagggattc gaagcggcgc ttgcctacgc catcgcggcc aagatgaact acacgcgtga
     5101 tgatgtgcga tgggtgcggg tgccgttcaa cgcggcgctg gctcccgggc caaagacgtt
     5161 cgacgccaac ctatctgagt tctcgatcac cgagcagcgc aagggggcgg tggatttctc
     5221 gtcaccgtat ttcgatgtga cgcaggcggt cgtcacggtc aagtcgtcgc ctgctgccgg
     5281 tgtcaagagg gtgcaccagc tgaagaagct gcgcctgggt gtccaagtgg gcaccaccag
     5341 ctatacggcg gcgacgtcag tagacggcga agtccctgtc gaggtctaca acaccaacgg
     5401 cgacgccaag ctggcgctga gcaacggcga gatcgatgca ctcgttgccg acctgccgac
     5461 cgcattcacg gtcgccaacg agctgcgcga cggcctgatg gtcggccaat tgccaagtgc
     5521 gtcaggcgat gtggagcagt tcggcgtcgt gctcgacaag ggcagcgggc tgacccgctg
     5581 cgtgtcgtgg gccgtcgact cacttcgcgc agagggcgca ctggatcggc tggaagaacg
     5641 ctggctcacc gatgccggca aggcgcccgt gctggcttag ctggccggcc ctggcgccag
     5701 tgatgagcac gcctgcatgg cgcggtgcca gatgtcctgg tcgacgccgg gcggcgggcc
     5761 aacggctgga cccggggcga tgggcacgcc gtgctcgctc aggcagtgcg cgaaaccgcc
     5821 gtggccggac ggggctacag atgtcgaaac ctgttctggc gacggcgtat tcgatgagca
     5881 cgcggcgaga attcccgccg cggcgacgac cgcggccagt gctatcgccc gcactatccg
     5941 acgaacctcg acagccgggc ggacagatgc gggaccagcc cgccgtcggc gtcgacccgt
     6001 tcctccacat aggccaggtc gccgccctcg acgatgccgt acagccgctt ggccccgccg
     6061 acgagcaccc ctgacttact gcgcgccagc gcgtcggtga ccagctccca cgacgactgc
     6121 gttctcggcc tgccgtaaaa caattcgacg tagcccgcag aatgcgcaag cagcagctcg
     6181 atggcttggg actcagaagg atcggctggg tcatcgacga accgccagaa gcccgactcg
     6241 cgcagtgtgg gttcgtcgta ctccccggtc tcggtcagcc gccacgagcg cgcttcccag
     6301 atgagatagt cgcgaccgtc atgcgagacg acgatctgct gaccgaaccg gtagtcaccg
     6361 ccgggtccgc ggccctcgcc ctcgccgcgc cacacgccca ccagcggtag cagcgcgagg
     6421 agcgcatcgt tgaggttcgc gccctcacgc aggttggcgg tatcggcggg cagcggtagg
     6481 tcgtcgaaaa ccgggatgtt gcgggccgcc gttgccttgg cgcgttcggc cgccgccgcg
     6541 acggcttcgt cgccggaggt cacgactcgt cggtgatgag gcggtacagc gtgtagagcg
     6601 cgaaccacgt gatgaccacg acggccgcaa cgagcatgat ctcgaagaac agcaccacgg
     6661 cagcgagtct agccgccgag ggcccatcaa ctcttggcgg ctgtcgtggc gtatctcacg
     6721 ggagttcgtc gacgtacttc gggcacagcg tgttcgtcgc ggcgcggacg aactggccgg
     6781 cgaggcgttg gttcccgccc attccgccgg cgacgccgcc aacggcgtcc gtgaaggcgg
     6841 cgcccgatgc gaagtcctgg cagaccatgt gcccgtgcgt gatgacttgc tgagtcgctt
     6901 tcgaggcgaa ggtgacgccg ccgttggcca aggcgtcgag gaactcctgg tcgggagtgg
     6961 ctgcggcaaa gggcgcggta gctacggtga tgaaggcgaa caccgtcgtg gcgaatccag
     7021 caattgcgat tctcatgatc ccccccgtta caagcattaa tttcgcgcag gagcgcagaa
     7081 actaaccact tggtggaggc agcgttttcg caacgcgggg ccggtgtggc cgttactggc
     7141 cacaccgggc gtcaggcgac cttgacgtcg acctcgtgga tgcccgcacc ggacggggcc
     7201 acgctggcgt cgccgttgcc cgccggggac agcgcgcgca gcgtccacgt gccgggagcg
     7261 gcgaagaacc ggaagtcacc ggtggccgac gcgacgacct cggcagtgaa ctcgtcagac
     7321 gagtccagca gacgcacgaa cgcgccgccg acggcctgac ccgcgccgtc gacaacgcgg
     7381 ccggtgatca ccgtttcctt ctcgaggtcg acgctggcgg gcaacgtcag gccttgcttg
     7441 ggtgcagagc acataatcaa cttcccaact cgatcgggac tcccacgagg gagccgtatt
     7501 ccgtccaact gccgtcatag ttcttgacgt tcttgtgtcc caacagttcc tgcagcacga
     7561 accaggtgtg cgacgagcgc tcaccgatcc ggcagtaggc gatggtctct ttctcgccgt
     7621 ccaggccggc ctcggcgtac aacgtggcca ggtcctcgtc cgacttgaat gtgccgtcct
     7681 cgttggccgc cttgctccac ggaacgttga gggcgccggg gatatgcccg gcgcgctggc
     7741 tctgctcctg gggcagatgc gcgggggcga ggatcttgcc ggagaactcg tcgggggagc
     7801 gcacgtcgac caggttcttc ttgctgatgg ccgcgatgac ctcgtcgcgg aacgcgcgga
     7861 tcgcgttgtt ggggtccttg gcggtgtagc tggtctccgg ccggttcacg gcatcggtgg
     7921 acagcggacg accgtcgagc tcccacttct tgcggccacc gtcgagcagc ttgaccttgt
     7981 cgtgcccgta cagcttgaag taccagtacg cgtacgcggc gaaccaattg ttgttgccgc
     8041 cgtagaggat cacggtgtcg tcgttggcga tgccgcgttc agacagcagc ttggagaact
     8101 gctgggcgtc gacgaagtcg cgcttgacgt ggtcctgcag atcctctttc cagtccaact
     8161 tgatggcgcc ctcgatgtgg ccgccgtcgt aggcgctggt gtcctcgtca acttcgacga
     8221 agacggtgtt cggcgcggtg agattgctct cggcccagtc tgtgctgacc aggacgtcgg
     8281 agcgtgccat ggacgaaatc ctttcggttg cttgctgttt gagcgttatg cgggggacgg
     8341 tgcacggcga aaccgtgcga cgagcggata gacctggcag ccgaggcaga tgccgaaggc
     8401 ggcgttgagg aacgcggcga cgagggcgaa gccggtcgcg accaaaccga gaagcggcac
     8461 accggcggcg aaaccgatgg tgccgacaac tgcgaatacg aagccgacca gttgggcgaa
     8521 cttcaggggt ggtacgggct ccttctcggt gacaggtccc agccggggcg cgacgaggct
     8581 ctgaaagatc agcccgtagg gatgctgccg cggcccgcgc caggcgccga tagcgaacac
     8641 cagcgcctgc agggcgagga tcacggccgc gctgctgctg ctgaaggccg agacaatcag
     8701 ggtcgcgacg atcaccaccg tgctgaccca ggcggcgaat cgcgggccac gcacgtcaac
     8761 cagggccggt gcggccgtct tggtgttagt cgacatagat gtgctcctgt gcgtcaatgg
     8821 gctggctgga agggagtcag accgctcgat ctgaaccccc gagtgcggcg cgaaggatcg
     8881 gcgcggctac tcagcagcta caacaacaac agcaacaacc cgctacgcgg cacagatcga
     8941 ctgcgcggcg cttcgtgagc atcggctcaa ggcgggtgga cacgcccgac agcttaccca
     9001 atgacgtagt gatcaagcca acagaggttc cagcgccgat cgcaggtcag cggccctggg
     9061 cacacccgtc gtccggtaac ggggccgccc gtcggcgtcg aagatgatcg tggtgggcag
     9121 cgacatcacc gaaagccgtc gcgcggcttg tggattggcg tccatgtcga tctccacgtg
     9181 cgccacgccg ggcaactcgt cacacacctg atccaccacg cggcgtaccc cggcgcacgg
     9241 tccgcaccaa tccgcgctga agtgcagcac ggtcgggccg gatgacgaaa ggcccagatc
     9301 ggacgtatcg acgttgctcg cctcttcggc agacttgagc atgccagcgc gaagtgtcag
     9361 cagcttgccg atcacatacg ccacacccag tgcggcgacg agcactgtga tgacgagaac
     9421 ccaagtagag ctcatgacag tcgaaaccca ttgaggggga tggttactcc cttggtgatt
     9481 ccctcaatga tgatgtccga accgcgcgca ccctcgctgg tcgggcggac gccgaacggc
     9541 agcttctgac caggcatgct gatggaaaac gcggcgagca ccgcgggcag cttgtcctcg
     9601 ggtacctgct ggtcggccgt gcccgcaccc gtcagcactc cggtcgcggt gaagaccagc
     9661 gtggtctggt cggctccggc gaccgacaaa tcgacggcga tgctgacctt ctcgtcgtag
     9721 tccgccttgt tgggcgtgcc ggtgaacacc agcccccggt tgctggagat gcccgattcg
     9781 gtggtcccgc cggtggcgtc gttgctctcc cgcgtcggcg cctcgaccag caggtccggg
     9841 atacccatga accgcccgac gtgggtggag tcgatgatga tgcggctctc cagctcgccg
     9901 accggcagct tggcgtctgg cccgataagc caggaggtct gtgtcagatc gatcgagtgc
     9961 agggtggcct ccaggcccac cttgccgacg acggggtgat caacgccact ggccctgatt
    10021 tcgacctcgt tgtagtggtc tcgcgtcgcc tgtgtgacga acgggaaccc gaggatgctg
    10081 acccaggggt cgaagttcag gtttgtggcg atgcgcacgc tcctggccag gtggtattcg
    10141 gcgtagatcg ccgcaccgaa gtcggctccg acggcgccca ccgccaccgc ggtcacggtc
    10201 gcgatcgagc cgatcagcag ctttcgcacc cggacattct ggcccacgcc cgccacaaac
    10261 ccggctcagc gcagctaacc agcaggtggc gcgctatcgt tagatgacca tcggccgggt
    10321 aacggccgtg tgtcaggcgt gaatctggga agtcaccacc gacatcgttg tccgggcgag
    10381 gtccctatgg ctgttggagg gccagttgga tctattgcta ctgaccgtcg acccccatcc
    10441 cgaatctgtg ctgccctcgt tgtcgctgct tgcgcacaac gtgcggacgg cgccgacgga
    10501 ggtgtcctcc cttctcgagg cgggcaccgc ggatgtggcg atcgtcgacg cgcgcactga
    10561 tctggccgct gcccgcgggc tgtgtcgcct gttgggaacg acggggacat cggtacccgt
    10621 ggtggccgtc gtcaacgagg gcggccttgt cgctgtaaac gtcgaatggg gactcgacga
    10681 gattctgctg ccgagcaccg gtcctgccga gatcgacgcc cggttgcggc tgctggtggg
    10741 gcgtcgcggc ggcgtcgcca accaggagaa cgtcggcaag atcagtctgg gcgagctggt
    10801 catcgacgag ggcacgtaca ccgcgcggct gcgcggtcga ccgctcgacc tcacctataa
    10861 ggaattcgag ctgctgaagt atctggctca gcacgccggg cgggtgttca cccgcgcgca
    10921 gctgctccag gaggtgtggg gctacgactt cttcggcggc acccgcaccg tcgacgtgca
    10981 cgtgcggcgg ctccgcgcca agctcggccc cgaatacgaa tcgttgatcg gcacggtgcg
    11041 caacgtcggc tacaaggcgg tccggcccgc gcgcggcagg ccgtcgacac caggctcgga
    11101 attgtccgac gacttcgtcg acgacgtggg tagctacgag gcgcccgacg gcctgcccga
    11161 cgcgctggta gacccattgc gtcagtgact gacattggtt ggcgcgcagg cctttccgac
    11221 gacgagcagc accgtatccg tgagctgatc gcggcggccg aacaggctga cgggatcgcg
    11281 cccgtcggcg accaggtgct ccgcgaacta ccgcacgacc gcaccaggca cctgcttgcc
    11341 gtcgacggcg ctgaggtcat cggctacctg aacctgaccg aacagcctgc gatggctgag
    11401 ctggtggtgc atccggacac caggcgccgc ggcgtcggct cggcgatggc ccggatcggg
    11461 ctgtccgagg gcggtgaccg cgcccgcatc tgggcgcacg gcaacttgga gccggcgcgg
    11521 gcgacggcgg ctgcgctcgg cctcgtagcc gaccgtgaac tgcttcagat gcgtcggcca
    11581 ctgaccggtt tgccgcctgc ctccgttccc gatggcgtcc gcatcacgac ttactctggg
    11641 ccggccgacg acgccgagct gctgcgcgtg aac
//
LOCUS       DASRJA010000585         4954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1960496,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000585 DASRJA010000000
VERSION     DASRJA010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960496"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..634
                     /locus_tag="VFC01_26015"
     CDS             119..634
                     /locus_tag="VFC01_26015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZN83107.1"
                     /translation="MPEGLTKQLGARARPPKDVQAITIDDVDRRILTALHAEARMSNN
                     ALAELVGIAPSTCHGRVRRLQELGVIRGFYADIDPAAIGLNLQAMISVSLQSNARGKI
                     RNFIQHIRTRPQVMDVYFLAGADDFILHVAARDTDDLRAFVVENLNADADVAGTQTSL
                     IFEHLRGASPL"
     gene            907..1215
                     /locus_tag="VFC01_26020"
     CDS             907..1215
                     /locus_tag="VFC01_26020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83108.1"
                     /translation="MKSFTLPAIAAGALATAALGLAGAAGAAPSGVVDAAQTVGQLQA
                     SGFDVIVNKLGTAPLDQCVVSAVRPGQTFSRMDSGAPGAMDDIVTTVTGMTVYVDVAC
                     "
     gene            complement(1236..1394)
                     /locus_tag="VFC01_26025"
                     /pseudo
     CDS             complement(1236..1394)
                     /locus_tag="VFC01_26025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1619..2356
                     /gene="dapB"
                     /locus_tag="VFC01_26030"
     CDS             1619..2356
                     /gene="dapB"
                     /locus_tag="VFC01_26030"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZN83109.1"
                     /translation="MRVGVLGAKGKVGATMVQAVEAAEDLTFSAGVDAGDPLSLFADN
                     QTEVVIDFTHPGVVMDNLKFLIDNGIHAVVGTTGFTDERLAQVRAWLADKPDVAVLIA
                     PNFAIGAVLSMHFAKQAARFFESAEVIELHHPQKADAPSGTAARTARLIAEARKDLPP
                     NPDATSTGIEGARGADVDGIPVHSVRLAGLVAHQEVLFGTQGETLTIRHDSLDRTSFV
                     PGVLLAVRTVRERPGLTVGLEPLLDLT"
     gene            2353..2817
                     /locus_tag="VFC01_26035"
     CDS             2353..2817
                     /locus_tag="VFC01_26035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN83110.1"
                     /translation="MSDGARALRIQLVIVFMCVALVVYFVMLGRIAMAFVTSGRPAAI
                     GLGLALMILPLIGIWVMVSTLRAGLAHQRLARIIKEEGMELDISSLPRMPSGRIQRDA
                     ADALFETVRDELDSDPDNWRRWYRLARAYDYSGDRGRARETMRKAVEMEERQ"
     gene            2859..3269
                     /locus_tag="VFC01_26040"
     CDS             2859..3269
                     /locus_tag="VFC01_26040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZN83111.1"
                     /translation="MQEMFEAVVSGATDPEIQGVEVVRRPALTVSPTDMLEADGYLLG
                     SPANLGYMSGALKHAFDVCYYPCLDTTRGRPFGLYLHGNEGTEGAEKGVTAITTGLGW
                     VKAAEYVLVSGKPGKDDLESCWELGASVAAQLME"
     gene            3404..3868
                     /locus_tag="VFC01_26045"
     CDS             3404..3868
                     /locus_tag="VFC01_26045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83112.1"
                     /translation="MPGIAELAMGAAPIAGGALLGVAAGNIKAPDVRGLILKDMDLLE
                     RLPDDRPELKARLKESIDQRINDLIVATERSRALREAAASYRGNWRDVVLFVCSVLFT
                     IIWWNVKHDRPNWLVLFIALVLLSLVTAIYAFRGAIMAIGRFFRKPRNAPTE"
     gene            complement(3858..>4954)
                     /locus_tag="VFC01_26050"
     CDS             complement(3858..>4954)
                     /locus_tag="VFC01_26050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HZN83113.1"
                     /translation="LTLLPLRALNRATLGRQLLLQRSPMSAIDAIEHLIGMQAQAPFA
                     PYYGLWSRLDRFTGEDLSRLLTARKAVRIVLMRGTIHLVTAKDCHRLRPLVQPALDRM
                     LRANATHGKPLAAIDVSAVVDAARQLLDAETLTPGEVGARLAERWPDTPPGALAEAAR
                     SLLPLVQVPPRALWQRSGQVRLTTATAWLGKPRAKPLTIDDLVMRYLAAFGPASAADV
                     QTWSGLTRLGEVLERLQLTRFKSEGGQTLYDIPDAPQPDAHIPVPVRLVAPFDNLLLS
                     HADRTRVISEEHRKRLFSGKNGVFPGTVLIDGFVAGTWELLGNGESTGLRVQPYVRLK
                     RGVLDEVELEGNRLLERAFGLPNRVVTITP"
BASE COUNT          878 a         1595 c         1594 g          887 t
ORIGIN      
        1 ctcggtcggg attccgacgc gcatgacggc tcctaaaagt cgtgttggct acctcagatt
       61 gtgaagaact ttcgctgtaa tggcaatcac cccgatgaaa cttcgatagc atcgtgtaat
      121 gccagaagga ttgacgaaac agcttggggc aagggcccgg ccgccgaagg atgttcaggc
      181 catcacgatc gacgacgtcg accggcgcat tctgaccgcc ctgcacgccg aggcgcgcat
      241 gtcgaacaac gcgctcgccg agctcgtcgg catcgcccca tcgacatgtc acggccgggt
      301 ccggcggctg caggaactcg gggtgatccg cggcttctac gccgacatcg atcccgcggc
      361 catcgggctg aacctgcagg cgatgatctc ggtcagcctg caatccaatg ctcgcggcaa
      421 gattcgcaac ttcattcagc acatccggac ccggccacag gtgatggacg tttacttcct
      481 cgccggagcc gacgacttca tccttcatgt cgccgcacgc gacaccgacg acctgcgggc
      541 attcgtcgtg gagaatctga acgccgacgc cgacgtggcg ggcacgcaaa cctcgctgat
      601 cttcgaacac ctccgcggag cctcaccgct ataggtcgct ataggtctcg tgtgtgctgc
      661 acgggcgggc ggcggagctg ggtgacattc acgacctact cgcggatgca ctggaaccca
      721 agaaatgcaa tggaattggg catgacaaca ccgcgtgctc ggtcgtcgaa ggtgggtaat
      781 cgccctccgc ggcgaatctg tcagatgacc actcaaccca tccaacgagg cagctgtaac
      841 gaatgaaggg catcagcaca cagcagtcag gaaaacgtca tcagctctct ctcgaaaggt
      901 agaacaatga agtccttcac cttgccggct atcgccgccg gagcactcgc cactgcggcc
      961 ttgggtttgg ccggcgccgc cggcgcggcg ccctcgggcg ttgtcgacgc agcccaaacg
     1021 gtcggccagc tgcaagccag cgggttcgat gtgatcgtca acaagctcgg tacggcgccg
     1081 ctggaccaat gcgttgtgag cgcggtacgg cctggtcaga ccttctcgcg gatggactcc
     1141 ggtgcacccg gtgcgatgga cgacatcgtc actaccgtca cggggatgac cgtgtacgtc
     1201 gacgtagcgt gctgaccgca tccacgccgg accggtcagt ccgcagtgag gtcgtcgcct
     1261 tcgtcgtctt ggtcgggcag taggtagttc tgcgggtggt ggtagtcgtt tacccgtgat
     1321 tgcccattgt cgagatgcgg cggtggaatc cattcggtgc ggccgtcttt gcgttttcgg
     1381 gtgcgccagc tgccgggaga cagtttccag ccggtctttg acggtcagcg cttccggtgc
     1441 gctcagcacc tgaaaggaca gtgctgcgac gttggcttgg gcggcgtcga actgatcgag
     1501 ggcggccgtg atcgcctcgc gacgcggcgg cacacccgaa ctcatatctc gaacactagt
     1561 tcgaatagcg gacaagggca gaacgttatc cacaggcggc ggaagttagg gtaggcccat
     1621 gcgagtcgga gtgcttggcg ccaagggcaa ggtcggcgcg acgatggttc aggccgtcga
     1681 ggccgcagaa gacctcacgt tttccgccgg ggttgacgcc ggcgatccgc tgagcctgtt
     1741 cgccgacaac cagaccgagg tcgtcattga cttcacgcac cccggtgtcg tcatggacaa
     1801 tctgaagttc ttgatcgaca acggaatcca cgccgtagtg ggcaccaccg ggttcactga
     1861 cgaacgcctg gctcaggtca gggcctggct cgcggacaag cccgatgtcg cggtcttgat
     1921 cgccccgaac ttcgcgatcg gcgcggtgct gtcaatgcat ttcgccaaac aggccgcccg
     1981 cttcttcgag tccgccgaag tcatcgaact gcaccaccca cagaaggccg atgcgccatc
     2041 cggcactgca gcccgcaccg cccgtctgat cgcggaggcc cgaaaagacc tgccgcccaa
     2101 cccggatgcc accagtacgg gcatcgaagg tgcccgcggc gcagacgtcg acggcatacc
     2161 ggtgcactcc gtgcggctcg ccgggctcgt cgcgcatcag gaagtgctct tcggtaccca
     2221 aggagagacc ctgacgattc ggcacgacag ccttgaccgc acatctttcg tgcccggtgt
     2281 gctgctcgcg gtccgcacgg tccgcgaacg ccccggcctc acagtgggtc tcgagcctct
     2341 tctcgacttg acatgagcga tggggcgcgc gcactgcgca tccagctggt gatcgtgttc
     2401 atgtgcgtgg ccctggtcgt gtacttcgtg atgctcgggc gcatcgcgat ggcgttcgtc
     2461 acctcgggcc gcccggcggc catcggtctc ggcctggcgt tgatgatcct gccgttgatc
     2521 gggatctggg tcatggtcag cacgctgcgg gccggccttg cccaccagcg gctggcccgc
     2581 atcatcaaag aggaaggcat ggaactcgac atcagctccc taccgcggat gccgtccgga
     2641 cggatccagc gtgacgccgc cgacgcgctg ttcgagacgg tccgcgacga actggacagc
     2701 gaccccgaca actggcggcg ttggtaccgg ctggcccgcg cctacgacta ttccggcgac
     2761 cgcggccgcg ccagggagac gatgaggaag gccgttgaga tggaggagcg acagtgacca
     2821 agacgctgct gatcgtccac cacactccgt cgccgttcat gcaggagatg ttcgaggcgg
     2881 tggtgtccgg cgcgaccgac cccgagatcc agggtgtgga ggtcgtgcgg cgacccgcgc
     2941 ttacggtctc gccgaccgac atgctggagg ccgacggcta tcttctcggc agcccggcaa
     3001 acctgggcta catgagcggt gctctcaagc acgcgttcga cgtctgttac tacccatgcc
     3061 tcgacacgac tcgtggccga ccgttcggcc tctacctgca cggcaacgag ggcaccgagg
     3121 gggccgagaa gggcgtcacc gccatcacga ccggcctggg gtgggtcaaa gccgccgagt
     3181 acgtcctggt gtcgggcaag ccgggcaaag acgacctgga atcgtgttgg gaacttggcg
     3241 cgtcggtcgc cgctcagctg atggaatgag ggcagactac cgacagcgcg atgggggtgg
     3301 ccgagatgat gaatgggttt gctcttgtgc tggcgaccgt ccttctgatg gtcgtgcaca
     3361 catggcggtt tcggtgacac attcgtgaag tgacggagga gacatgccag gtatcgccga
     3421 actcgctatg ggcgcagcgc cgatcgccgg cggagcactg ctcggtgtcg ccgcgggaaa
     3481 tatcaaggcg cctgatgttc gcggtctgat cctcaaggac atggacctgc tggagcggct
     3541 tccggatgac cggcccgaac tgaaagcccg cctgaaggag agcatcgacc aacgcatcaa
     3601 cgacctgatc gtcgcaaccg agagaagccg cgcgctgcgc gaggccgccg cgtcctatcg
     3661 cggtaactgg cgtgacgtgg tgctgttcgt ctgttcggtc ctcttcacga tcatctggtg
     3721 gaacgtcaaa cacgaccggc cgaactggtt ggtgttgttc atcgccctgg tgttgctgtc
     3781 gctggtgacc gcgatctacg cgttccgagg cgctattatg gcgatcggca gattctttcg
     3841 caaaccgcgt aacgctccta cggagtgatc gtcaccaccc ggttcggaag cccaaatgcc
     3901 cgctccagca aacgatttcc ctccaactcg acctcatcga gcacgccgcg cttgagcctc
     3961 acatacggct gtacccgcaa gccggtcgat tcaccattgc cgagcagctc ccacgtgccc
     4021 gcgacgaaac cgtcgatcag caccgtgccc ggaaacactc cgttcttgcc agagaacaac
     4081 cgcttgcggt gctcctcgga gatcacgcgg gtgcggtcgg catgtgacag caggaggttg
     4141 tcgaacggcg cgacgagccg caccggcacc ggtatgtgcg catcgggttg tggcgcgtcc
     4201 ggtatgtcgt aaagcgtctg tccgccttcg cttttgaacc gcgtcaactg cagccgctcg
     4261 agtacttcgc ccagtcgcgt gagcccgctc cacgtctgca catctgcggc actcgccggg
     4321 ccgaacgccg cgaggtagcg catcaccagg tcatcgatcg tcagtggttt ggctcgcggc
     4381 ttgccgagcc aggcagttgc cgtcgtcagc ctgacctgcc cgctgcgctg ccacagtgcc
     4441 cgaggcggga cctggaccaa cggcagcagg ctgcgcgcgg cctcggcaag cgcacccggc
     4501 ggcgtgtccg gccatcgctc ggcgagccgg gccccgactt cgcctggcgt cagcgtctcg
     4561 gcgtcgagaa gttgtctggc cgcgtcgacg accgctgaca catcgatggc cgcaagcggc
     4621 ttgccgtgcg tggcgttcgc ccgcagcatg cgatccagtg ccggctggac gagcgggcgc
     4681 agccggtggc agtccttggc cgtcacgagg tggatggtgc cgcgcatcag cacgatccga
     4741 acggccttgc gtgccgtcag cagccgcgac aggtcctcac ccgtgaagcg gtccagccgg
     4801 ctccacaacc cgtagtacgg cgcgaacggt gcctgcgcct gcatgccgat gaggtgttcg
     4861 atcgcgtcga tcgcgctcat cggcgagcgc tgcaagagca gctggcggcc gagtgtggcc
     4921 cggttcagcg cccgcagcgg gagcagcgtc aatt
//
LOCUS       DASRJA010000586         9897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2698570,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000586 DASRJA010000000
VERSION     DASRJA010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2698570"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFC01_26055"
     CDS             <1..287
                     /locus_tag="VFC01_26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83114.1"
                     /translation="RHHTSELRFLVHWSAELFSDYDELKKNMDGSDDLTVERAIDTLV
                     KDVNANGLKLDMPDDPLHDPVFIRTLNAAYDLGGPASYPDEAPLSAFQMA"
     gene            complement(291..1100)
                     /locus_tag="VFC01_26060"
     CDS             complement(291..1100)
                     /locus_tag="VFC01_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN83115.1"
                     /translation="MAHSSQAVAVVTGASRGAGRGIAAALVAHGWRVYLTGRTVTDPG
                     DGGIAVPVDHSDDAAVAALFEHVADESGRLDLLVNNAAAIHDELTNPKPFWEKPLELG
                     DVLDVGLRSAYVATWHAAPAMVRQGRGLIAFTSSPGSVCYMHGPAYGAQKAGVDKLAA
                     DMAVDFRDTGVATVSIWMGILLTERFRGAFAGHPEALAETAKHAETPEFTGHLINALY
                     HDPALAELSGHTVIGAELAQRYGVTDEDGRQPPSHREMLGAPREPSTVIVR"
     gene            complement(1111..1401)
                     /locus_tag="VFC01_26065"
     CDS             complement(1111..1401)
                     /locus_tag="VFC01_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN83116.1"
                     /translation="MAVRPDNRLADSPMVPVCCRRCGASVLARKSSWNQTSVQWDAEA
                     SARCLERREAENLAAYAGRSLFLACSALSDAIIEAVRHGDLPVVDETVPATT"
     gene            complement(1401..2540)
                     /locus_tag="VFC01_26070"
     CDS             complement(1401..2540)
                     /locus_tag="VFC01_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014382704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN83117.1"
                     /translation="MTLQSDTDETRQIEAEAAPTRFARGWHCLGLTRDFGDGKPHAVH
                     AFGHKLVVFRGGDGRINVLDGYCRHMGGDLSQGEVKGDEIACPFHDWRWGGDGRCKKV
                     PYSKRVPKLARTATWTTLEQDGMLFVWNDPERKPPPPEVTIPRIEGATSDEWTDWHWY
                     TTVVNINCREIIDNVVDMAHFFYIHGSLPTHFKNIFEGHVATQYMNGAGRPDMGEPGK
                     PQMLGTTSVASYFGPSFMIDDLTYHYEEADHQTVLINCHYPIDANSFVLQYGIIVKKS
                     DALPGDLALETAIGLGDYVKMGFEQDVEIWKNKARIDNPLLCEEDGPVYQLRRWYQQF
                     YVDVADVAPDMVDRFEYELDTTSPREEWMKVVEGNLADRSAAGTT"
     gene            2606..4345
                     /locus_tag="VFC01_26075"
     CDS             2606..4345
                     /locus_tag="VFC01_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketosteroid-delta-1-dehydrogenase"
                     /protein_id="HZN83118.1"
                     /translation="MRARFCDLVSSNVTTAQPATIPVGLPVHDTTVDLLVVGSGTGMA
                     AALAAVERGLKVLIVEKSEYVGGSTARSGGALWLPASPILEENRTGDTIPRAGTYLES
                     VVAGTAPPQRSTEFLAHLGETVDMLRRTTPMKFIWARDYSDYHPENPGGTAAGRTCEC
                     RPFDTSKLGPYRSRLRPGVMKASIPMPTTGADYRWMNLMARVPRKGLPLIAKRLGQGV
                     GGLLLGRHYVAGGQALAAGLFAGVLRAGIGIWTDTPLVRLITDGGRVTGAVVDHDGRE
                     VAVTARRGVVLAAGGFDHSMDMRWKFQSESLGEHASLGAETNTGDAIRIAQDAGAGID
                     LMDLAWWFPAVAPLPGAAPLVMLAERSLPGSLIVDQTGNRFTNEAADYMSFGQHVLDR
                     ERSGNPVESMWIVFDQQYRNSYVFAAELYPRMRLPQSWYDAGIAHQSGSMAELARMIG
                     VPEAPFVATVTRFNEMSRAGDDSEYHRGRSAYDRYYGDPTITPNPNLRALDKGPFYAV
                     KMVLSDLGTCGGLRADERARVLREDGGVIDGLYAVGNTAANAFGTTYPGAGATIAQGL
                     VYGYIAARDAAAK"
     gene            complement(4346..5017)
                     /locus_tag="VFC01_26080"
     CDS             complement(4346..5017)
                     /locus_tag="VFC01_26080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZN83119.1"
                     /translation="MVDSDQPGKPNLAATSWALLGMLSYEYELSGYDIRKWIEWSMRF
                     FYGSPAYSQIYSELKKLEKLGLVSSRVESASSRNRRLYKITDAGLDAVTKWANEAPLD
                     PPVLKHGPLLRVTLAHLTTPARLKEILQEHLAYADAMHRNAAKDAKWAGADPSWAYAR
                     VALQWAERYYANERELTLKMIKELDEAEATFPRAGDGGRAKIPWPTPEYWYEIEKKAD
                     ADESG"
     gene            5135..5620
                     /locus_tag="VFC01_26085"
     CDS             5135..5620
                     /locus_tag="VFC01_26085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007537432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZN83120.1"
                     /translation="MTSCAPPTSTSMRDVLGRFCTGIAVITASDGDRPYGFTCQSVTS
                     VSLDPPYISFCPARTSKTWPMIREVGALCVNILAHDQHHVCTQFAVSGADKFSSVRWQ
                     LAANGAPLIEGAVASIEANVEFEHGAGDHTIVVAHVTGLHASDERRPLLFYRGGYGGF
                     A"
     gene            5617..6192
                     /locus_tag="VFC01_26090"
     CDS             5617..6192
                     /locus_tag="VFC01_26090"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN83121.1"
                     /translation="MTTHETLTASVRRLIDATIRTEVDAAAIVAAKNKIDSATAQLSD
                     MLMPGSFGVQLTHDGQSIALGNVVIGQRNPVAPPLVIRDEADGTVCTNFVLGAAYEGP
                     PGMLHGGVCALILDHVLGATAHLPNKPAYTGTLTLRYLRGTPLGQPLCARAHVERIDG
                     AKTFAVGHIADAEGVTVQAEGVFIHPRKAQR"
     gene            6189..7736
                     /locus_tag="VFC01_26095"
     CDS             6189..7736
                     /locus_tag="VFC01_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZN83122.1"
                     /translation="MTTWADETDVIVAGSGGGLAGAYTAAREGLSVMVVEASDEFGGT
                     TAYSGGGGMWFPCNPVLRRAGSDDTLDAAFAYFHAVVGDRTPVELQRAYVYGGSELIA
                     YLEEDDHFEFDVLPWPDYYGRLPHARLDGLRHTVMKPLAAKRLGELAQRIRGPLDRER
                     LGAEAPNYLIGGRALIGRLLSAITTYPHASLHLNSPLVELITADDGVVGAVVEEDGRR
                     RAIRARAGVLLAAGGFENNPALRQRFGVPGDARDTMGAPGNLGLALEAGLAAGAAIDL
                     MDQAWWSPGFTHPDGRSAFSLWFTGGIFVNQDGKRFVNESGPYDRIGKAIIDQIACGA
                     MTLPFWMAYDDREGLVPPVKATNISMVEAERYVEAGLWHTADTLPELASKIGVNAENL
                     VSTVKRFNEFVAAGTDEDFGRGDEAYDRAFSGGRSPMVSIEVAPYHAAKFGLSDLGTK
                     GGLVTDSHARVLDTNGNPIDGLYAAGNTMAAVSGTAYPGGGNPIGASMLFSHLAALDM
                     ATKGTRG"
     gene            7768..8574
                     /locus_tag="VFC01_26100"
     CDS             7768..8574
                     /locus_tag="VFC01_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN83123.1"
                     /translation="MASLKRYADRRVLITGGGSGIGQATVLRILDEGGRVVAADISES
                     GLKDTVARAEPHGDRLSTVVIDVASEESVKEGVAEAVRVLGGLDALVNAAGILRSAHF
                     LHTTLADFEQVLRINLVGTFLVIREAIPALRNGNDAAVVNFSSTSAGFAHPYMSAYAA
                     SKAGVQAMTHALALEFAKERIRFNSVQPGSISSGMTDGTGESKQSIGPGLPTDADYSL
                     FGKITPVLPLDDGGMFAKPDAVAAVVAMLGSSDAYFVTGTEVRIDGGTHM"
     gene            8585..9637
                     /locus_tag="VFC01_26105"
     CDS             8585..9637
                     /locus_tag="VFC01_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN83124.1"
                     /translation="MNTVDRPIRVIQWTTGNIGRRSLHAIIGRDDMELVGVYAHGAGK
                     VGVDAAELSGWPEPTGITATNDIDALLALKPDACCYNPLWPNIDELARLLEAGVNVCS
                     SAAWITGGKQSPEDLERIRKACEKGNSTIFGSGAHPGMTNMVGMVLSGSCERVDEIRI
                     TESVDCSTYESAGTQTAMGFSQDPDTPGLAESVRRESEVFAESAAMMADAIGAKLDKM
                     TFDVTFTPATGDTDLGFMQIPKGTVASVYGYHRGWVGDRNVVSVGFNWTMGDHVTPPK
                     PLEHGHVIQVFGLPNMRTVLHCLPPKDWTEPGFMGLGMIYTAMPVTNAVPAVVAAKRG
                     IVTLADLPPITGRSAV"
     gene            complement(9615..>9897)
                     /locus_tag="VFC01_26110"
     CDS             complement(9615..>9897)
                     /locus_tag="VFC01_26110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83125.1"
                     /translation="SVRASAMAVTNSIGRLCTAAGPLVAGAIATGWFDGDIAMAATVI
                     SALIVIAFIGLAFLPETNGRFLHSEVEKEAGADAGESVSPMSAIPLSDR"
BASE COUNT         1728 a         3290 c         3155 g         1724 t
ORIGIN      
        1 cccggcatca caccagcgag cttcggttct tggtgcactg gtcagccgag ctcttctctg
       61 actacgacga gctgaaaaag aacatggacg gctccgacga cctcactgtg gagcgggcga
      121 tcgatacgct cgtcaaggac gtcaacgcca acgggctcaa gctcgacatg cctgacgacc
      181 cgctacatga cccggtattc attcgcaccc tcaacgcggc gtatgacctc gggggccctg
      241 cgagctatcc agacgaggcg ccgcttagcg cctttcagat ggcctgacgc tcaacgcacg
      301 attacggtgc tcggctctcg cggcgcgcca agcatctcgc ggtgcgacgg cggctggcga
      361 ccgtcctcgt cggtgacgcc gtagcgctgc gcgagttccg ccccgatcac ggtgtgcccg
      421 gacagctcgg cgagggcggg gtcgtgatac agcgcattga tcaaatgccc tgtgaactca
      481 ggtgtttcgg cgtgttttgc ggtctcggcg agagcctccg gatgtcccgc gaatgcgccg
      541 cggaaccgtt cggtgagcag aatccccatc cagatcgaca ccgtcgccac cccggtgtcg
      601 cggaagtcga ccgccatgtc ggcggccagt ttgtccacac ccgccttctg cgcgccgtac
      661 gccgggccgt gcatgtagca caccgatccg ggtgacgacg tgaacgcaat cagaccgcgg
      721 ccctgccgca ccatcgcagg cgcggcatgc cacgtcgcca cataggccga gcgcagcccg
      781 acgtcgagaa cgtcgcccag ctcgagtggc ttctcccaga acggctttgg atttgtcagc
      841 tcgtcatgaa tggccgcggc gttgttgacc aacagatcga gccggccact ctcgtccgcg
      901 acatgctcga acagcgcggc aaccgcggcg tcgtcgctgt ggtcgacggg caccgcaatt
      961 ccgccgtcgc cggggtcggt caccgttctg ccggtcagat acacccgcca accgtgggcg
     1021 acgagcgccg ccgcgatacc gcgtcctgcg ccgcgactcg cccccgtcac gaccgccacc
     1081 gcctgcgaac tgtgtgccac ggatacgaag ttacgtcgtc gccggcaccg tttcgtcgac
     1141 cactggcaga tccccgtggc gtaccgcctc gatgatcgcg tcgctcaggg ccgaacaggc
     1201 aaggaacaga ctgcggcccg cgtacgcggc caagttctcg gcctctcgcc gctcgaggca
     1261 gcgcgcggac gcctcggcat cccactgcac gctggtctga ttccagctgc tcttgcgcgc
     1321 caatacagac gcaccgcagc gacggcagca caccggcacc atcggagagt cggcgagtcg
     1381 gttgtctggc cggaccgcca tcaggtggta cccgcggcag acctgtcggc gaggttgccc
     1441 tcgaccacct tcatccattc ctcgcgcggg ctggtggtgt cgagctcgta ttcgaagcgg
     1501 tccaccatgt cgggcgccac gtcggcgacg tcgacgtaga actgctgata ccaccggcga
     1561 agctggtaga cgggtccgtc ctcctcacac aacagcggat tgtcgatgcg cgccttgttc
     1621 ttccagatct cgacgtcctg ctcgaagccc atcttgacgt aatcccccag gccgatagcg
     1681 gtctcaaggg cgagatcgcc tggcagtgcg tccgacttct tcacgatgat gccgtactgc
     1741 agcacaaagg aattcgcgtc gatcggatag tgacagttga tcagtaccgt ctggtgatcg
     1801 gcctcctcgt agtggtaggt caggtcgtcg atcatgaacg acggcccgaa atacgatgcc
     1861 accgaagtgg ttccgagcat ttggggtttg cctggctcgc ccatgtcagg acgccctgcg
     1921 ccgttcatgt attgggtcgc gacatggcct tcgaagatgt tcttgaagtg cgtcgggaga
     1981 gatccgtgga tgtagaagaa gtgcgccatg tcgacgacgt tgtcgatgat ctcgcggcag
     2041 ttgatgttga cgacggtggt gtaccagtgc cagtcggtcc actcgtcgct agtcgcgccc
     2101 tcgatccgag gaatggtcac ctccggcggt ggcggcttgc gctcggggtc gttccacacg
     2161 aacagcatcc cgtcctgctc cagcgtggtc cacgtcgccg tacgcgctag cttgggcacc
     2221 cgcttgctgt acggcacctt cttgcaccgg ccgtccccgc cccagcgcca gtcgtggaac
     2281 ggacacgcga tttcgtcgcc cttcacctct ccttgggaga ggtcgccgcc catgtgacgg
     2341 cagtatccgt cgagcacatt gatccggccg tccccgcccc ggaacaccac cagcttgtgc
     2401 ccgaaggcat gcacggcgtg cggcttgccg tcgccgaaat ccctggtcag tcccagacaa
     2461 tgccagccac gggcgaatct ggtcggtgcc gcttcggcct cgatctgacg agtctcatcg
     2521 gtatcggact gcaacgtcat gaccttgatc tacaaccgca gcaccgggga cggagccgat
     2581 cgtcccgctg gcagggaaaa acccgatgcg tgcacgattc tgcgacttag tgtcgtcaaa
     2641 cgtgactact gctcaaccag ccaccatccc cgtcggcctg cctgtccacg acaccaccgt
     2701 ggacttgcta gtcgttggct cgggcactgg catggccgcg gcattggcgg ccgtcgagcg
     2761 tggtctgaag gtgctcattg tcgagaagtc cgagtacgtg ggcgggtcga ccgcccgttc
     2821 cgggggcgcg ctgtggctgc ctgcgagccc gatcctcgag gagaaccgca ccggcgacac
     2881 gatcccacgt gccggcactt atctggagtc cgtcgtcgca ggcaccgcac cgccacaacg
     2941 gtccacagaa ttcctcgccc atctcggcga gaccgtggac atgctgcggc gcaccacgcc
     3001 catgaagttc atctgggcgc gtgactactc cgattaccac ccggagaacc ccggaggaac
     3061 tgccgccggg cgaacatgcg agtgcaggcc gttcgacacg tcgaagctcg gtccgtaccg
     3121 atcgcgcctg cgtcccggcg tgatgaaggc atcgataccc atgccgacca ccggcgccga
     3181 ctaccggtgg atgaacttga tggcgcgggt gcctcgcaag ggtctaccgc tcatcgccaa
     3241 gcggctgggc cagggtgtcg gcggcctgtt gctcggccgt cactacgtcg ccggtgggca
     3301 ggcgctggcc gcgggcttgt tcgcaggcgt actgcgagcg ggcatcggaa tctggacgga
     3361 cacaccattg gtgcggttga tcaccgacgg tggccgggtc accggcgcag tggtggatca
     3421 cgacgggcgc gaggtcgccg tcaccgcgcg acgcggggtt gtcctggccg caggcggttt
     3481 cgaccacagc atggacatgc ggtggaaatt tcagtcggaa tcgctcggcg agcatgccag
     3541 cctgggcgcg gagaccaaca ccggcgatgc catccgaata gcccaagatg ccggtgccgg
     3601 aatcgatctc atggacctgg cgtggtggtt cccggctgtg gcaccgctgc ccggtgctgc
     3661 cccgctggtg atgctggcag agcgatcgct tcccggctcg ctgatcgtcg atcagaccgg
     3721 caaccggttc accaatgagg cggccgacta catgtccttc gggcagcatg tgctcgaccg
     3781 cgaacgctcc ggcaatcccg tcgagtccat gtggatcgta ttcgaccagc agtaccgcaa
     3841 cagttatgtc tttgcggcgg aactatatcc gcgcatgcgc ctcccccagt cctggtacga
     3901 cgcagggatc gcacaccagt ccggcagcat ggctgaactc gcgcgaatga tcggagttcc
     3961 cgaggcaccg ttcgtggcaa ccgtgacgcg attcaacgag atgtcgcgcg ccggagacga
     4021 ttccgaatat caccgcgggc gcagcgcata tgaccgctac tacggcgatc ccaccattac
     4081 ccccaatccg aatctccggg ccctcgacaa ggggcccttc tacgcggtga agatggtgct
     4141 cagcgacctc ggcacctgcg gtggcctccg agcggacgaa cgcgcacggg tgctgcgaga
     4201 ggacggcggc gtcatcgatg gtctgtacgc cgtcggcaac accgcggcca acgcgttcgg
     4261 aaccacctat cccggtgcgg gcgcgacgat cgcgcagggt ttggtgtacg ggtacatcgc
     4321 ggcccgcgat gcggcggcaa agtagtcagc cggattcgtc ggcgtccgcc ttcttttcga
     4381 tctcgtacca gtattccggt gtaggccaag ggatcttcgc gcgtccgccg tcaccggccc
     4441 gggggaaggt ggcctccgcc tcgtcgagct ccttgatcat tttcaaggtc agctcacgct
     4501 cgttggcgta gtagcgctcg gcccactgca gcgcgacgcg cgcgtatgcc catgacgggt
     4561 ccgccccagc ccactttgcg tccttggccg cattgcggtg catcgcgtcg gcataggcga
     4621 ggtgttcctg cagaatctcc ttcagcctgg ccggagtcgt cagatgtgcc aatgtcaccc
     4681 gcagcagggg gccgtgcttg agcaccggcg ggtcgagggg cgcttcgttc gcccacttcg
     4741 tgaccgcgtc cagccccgca tcagtgattt tgtacagcct ccgattgcgg ctgctggcgc
     4801 tctcgacgcg cgaggacacc aagccgagtt tctccagctt cttgagttcg gagtagatct
     4861 ggctgtacgc gggactgccg tagaaaaacc gcatgctcca ctcgatccac tttcggatgt
     4921 cgtagcccga aagctcgtac tcatacgaga gcatgccgag cagcgcccag ctggtggccg
     4981 ccagattcgg cttgcccggc tgatcgctgt ctaccacttc gccagattaa caacacaggc
     5041 cacagcggtg ttgtcactca ccactggccg ggacgctctg ttgcggcgcc gcagcgcagc
     5101 ggcgaaagct tacgtcgaaa acgaaggaag accgatgaca agctgtgctc cgcccacatc
     5161 cacgtccatg cgcgacgtcc tcggcaggtt ctgcacggga atcgccgtga tcaccgcgag
     5221 tgacggcgac cggccgtacg gctttacgtg tcaatcagtg acgtcggtat cgctcgatcc
     5281 tccatacatc tcgttctgcc ccgcccggac gtcgaagacc tggccgatga tccgtgaggt
     5341 cggcgcgttg tgcgtcaaca tcctggccca cgaccaacac cacgtctgca cacagttcgc
     5401 ggtcagcggc gctgacaagt tcagcagtgt gcggtggcag ctggcagcca acggcgctcc
     5461 gctgatcgag ggggccgtgg ccagcatcga agcaaacgtc gaattcgaac acggggcggg
     5521 cgatcacacc atcgtggtgg cgcacgtgac cggactccat gcttcggacg aacggcgtcc
     5581 cctcctcttc taccgcggtg gctacggagg atttgcatga ccacccacga aacgctcacc
     5641 gcatcggtgc gtcgtctcat cgacgcgacc atcaggaccg aagtcgatgc ggcggcaatc
     5701 gtggccgcca agaacaagat cgactcggcc acagcacagt tgagcgacat gctgatgccg
     5761 ggatcattcg gcgttcagct cacacacgac gggcaatcca tcgcgttggg caacgttgtc
     5821 atcggccaac gcaatccggt cgcgcctccg ctggtgatcc gcgatgaggc cgacggcacg
     5881 gtgtgcacga acttcgtgtt gggcgccgcc tacgagggac cgcccggcat gcttcacggc
     5941 ggtgtatgcg cgctgatcct cgatcacgtc ctgggtgcca ctgcacatct gccgaacaag
     6001 cccgcctaca cgggcacgtt gaccctgcgg tacttgcgcg gcacaccgct cggacagccg
     6061 ctgtgcgcac gggcgcatgt cgagcggatt gacggggcca agacattcgc agtcggccat
     6121 atcgctgacg ccgagggcgt gaccgttcag gctgagggcg tgttcatcca tccaaggaaa
     6181 gcccagcgtt gacgacctgg gcagacgaaa ccgacgtcat tgtcgccgga tccggtggtg
     6241 ggctggcggg cgcgtacacc gcggcgcgtg aagggctgtc ggtcatggtc gtggaggcca
     6301 gcgacgagtt cggcggcacc acggcatatt cgggtggcgg ggggatgtgg ttcccctgca
     6361 atccggtgtt gcggcgggcc ggaagcgacg acaccctcga cgccgctttc gcctacttcc
     6421 acgccgtggt cggcgatcgc accccggtcg agttgcagcg cgcctacgtc tacggcggca
     6481 gcgagctgat cgcgtacctc gaagaagacg accacttcga attcgacgtg ctgccctggc
     6541 cggattacta cggccgcttg ccgcacgccc ggttggacgg actgcggcac acagtcatga
     6601 aaccccttgc tgccaaacgc cttggcgagt tggcacaaag gatccgcgga cctttggaca
     6661 gagagcgcct cggcgcagag gcaccgaact acttgatcgg cgggcgtgcg ttgatcggcc
     6721 ggttgctgtc agcgatcacg acgtatccgc acgcgtccct tcatctcaac agcccgctgg
     6781 tcgagttgat caccgcggac gacggtgttg tgggtgccgt ggtcgaggag gacggccgtc
     6841 gccgcgcaat ccgggcccgc gccggtgtgc ttctcgccgc gggcggtttc gagaacaacc
     6901 cggcgctgcg ccagcggttc ggcgtgcccg gtgatgcccg ggacacgatg ggcgcgccgg
     6961 gcaacctcgg cctggcgctc gaggcgggcc tcgccgcagg cgccgcgatc gacctgatgg
     7021 accaggcctg gtggtcgccg ggattcacac atcccgatgg gcgctcggcg ttctcgctgt
     7081 ggttcacggg tgggatcttc gtcaatcagg acgggaaacg tttcgtcaac gagtcaggcc
     7141 cgtacgaccg gatcggcaag gccataattg accagatcgc ttgcggcgca atgaccttgc
     7201 cgttctggat ggcctacgac gaccgagagg gtctcgttcc gccggtgaag gcaacgaaca
     7261 tctcgatggt cgaggccgaa cgttacgtcg aggccggcct gtggcatacc gcggacaccc
     7321 tgcccgaact cgcatcgaag atcggtgtga atgccgagaa cctggtgtcg acggtgaagc
     7381 ggttcaacga gttcgtcgcg gcgggtaccg acgaggactt cggccggggc gacgaggcct
     7441 acgaccgcgc gttctccggc ggtcggtcac cgatggtgtc gatcgaggtc gctccctatc
     7501 acgccgccaa gttcgggctc tccgatctgg gcaccaaggg tggactcgtc accgacagcc
     7561 acgcccgcgt gctcgacacc aacggcaatc cgatcgatgg gctctacgcc gccggcaaca
     7621 ccatggccgc cgtcagcgga accgcctatc ccggcggcgg caatccgatc ggggcatcga
     7681 tgctgttcag ccacctcgcc gccctcgaca tggccacgaa gggcacacgt ggctgacaat
     7741 ccgtatctca cccaggagga tctgtccatg gcatctctca agcgatacgc cgaccgtcgc
     7801 gtcctgatca ccggcggcgg atccggtatc gggcaagcga ccgtactacg cattctggac
     7861 gaaggcggcc gagtggtcgc ggccgacatc agcgagtccg ggttgaagga cacggtggcc
     7921 agggccgaac cgcacggtga ccggctgagc acggtggtca tcgacgtcgc gagcgaggag
     7981 tcggtgaagg aaggagtggc cgaggcggtg cgggtgctcg gcggtctcga cgccctcgtc
     8041 aacgccgcgg ggattctgcg gtcagcgcat ttcctgcaca ccaccctcgc cgatttcgag
     8101 caggtgctgc ggatcaacct cgttggcacg ttcctggtga tccgcgaggc gatccccgcg
     8161 ttgcgtaacg gcaacgacgc cgcagtggtc aacttcagct ccacctccgc ggggttcgcg
     8221 cacccgtaca tgtcggctta cgccgcgtcc aaggctggcg tccaggcgat gacgcacgct
     8281 ctggcgctcg agttcgccaa ggagcgcatc cggttcaatt ccgtccagcc cggatccatt
     8341 tcctccggaa tgaccgacgg cacgggcgaa tccaaacaaa gcatcggtcc ggggctgccg
     8401 accgacgcgg actactcgct gttcggcaag atcacaccgg tgctgccact cgacgacggc
     8461 gggatgttcg ccaagccgga tgcggtcgcc gccgtcgtcg ccatgctcgg ctcgtccgac
     8521 gcctatttcg tcaccggaac agaggtgcgc atcgacggcg gaacgcacat gtagattgct
     8581 gcaaatgaac accgttgacc gacccattcg agtgattcaa tggaccaccg gcaacatcgg
     8641 acggcgatcg ctgcatgcga tcatcgggcg cgacgacatg gaactggtcg gcgtgtacgc
     8701 gcacggagcc ggcaaggtcg gcgtcgacgc cgccgagttg tccggctggc cggagcccac
     8761 cgggatcacc gccaccaacg acatcgatgc gctgctcgcc ttgaagcccg acgcctgttg
     8821 ttacaaccca ctgtggccga acatcgacga gctcgcccgg ctgctcgagg ccggcgtcaa
     8881 cgtgtgctcg agcgcggcgt ggatcaccgg cggcaagcag tcaccggagg atctggaacg
     8941 catccggaag gcttgtgaga agggcaattc cacgatcttc ggcagcggcg cccatccggg
     9001 catgacgaac atggtcggca tggtgctcag cggttcgtgt gagcgcgtcg acgagatccg
     9061 catcaccgag tcggtggact gctcgaccta cgaatcggcg ggcacccaaa ccgcgatggg
     9121 cttctcgcag gatcccgaca cgcccggcct tgccgaaagc gtccgacggg aaagcgaagt
     9181 cttcgccgag tcggccgcga tgatggccga cgcgatcggc gccaagctcg acaagatgac
     9241 gttcgacgtc acgttcacgc ccgcgaccgg tgacaccgac ctcggattca tgcagatccc
     9301 gaagggcacc gtggcgagtg tgtacggcta ccaccgcggg tgggtgggcg accgcaacgt
     9361 cgtcagcgtc ggcttcaact ggaccatggg cgaccacgtc accccgccaa agccgctgga
     9421 gcacggccat gtcatccagg tgttcgggct gcccaacatg cgcaccgtgc tgcactgcct
     9481 gccgccgaag gactggaccg agcccgggtt catgggcctc ggcatgatct acaccgcgat
     9541 gccggtgacc aacgccgtgc ccgcggtcgt cgctgcgaaa cggggcatcg tcacgctggc
     9601 cgacctgcca ccgatcaccg gtcgctcagc ggtatagcgg acatcggcga caccgactct
     9661 cctgcgtctg cgcccgcctc cttctcgacc tcggagtgca ggaagcggcc gttggtctcg
     9721 ggaaggaacg ccagtccgat gaacgcgatc acgatcaggg ccgagatcac cgtggccgcc
     9781 atcgcgatgt ccccgtcgaa ccagcccgtc gcgatggcac cggccacgag tgggcctgcg
     9841 gccgtgcaca accggccgat gctgttggtg accgccatcg cggatgcccg cactgac
//
LOCUS       DASRJA010000587         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_158422,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000587 DASRJA010000000
VERSION     DASRJA010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158422"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1586
                     /locus_tag="VFC01_26115"
     CDS             171..1586
                     /locus_tag="VFC01_26115"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZN83126.1"
                     /translation="MQMLTVAGLAMAVVWLAGVAPRAQSQVPSPAGRPNVVLIITDDV
                     GYGDIGSYGAPDIKTPNIDGLARGGVRLTDFYANATLCSPTRAGLVTGRYQQRFAIEA
                     ALGGVSTAEEQGLPASGRSLPQLLKTSGYATALLGKWHLGYKPEFSPNAHGYDYFFGF
                     KSGYIDYYQHTDGAGLPDLFENAAPVQQAGYMTDLITAKTVRFIEQNAGKPFFAEVAY
                     NAGHWPYQVPDKPSVAIGNGRHLMPHDATSTRGDYVAMMERADQGVGEILRTLDRLNL
                     ARNTIVIFTNDNGGEWLSRNAPLFNRKWTLWEGGIRVPAIVRWPGRIPAGRVSPQVGI
                     TMDFTVSILAAAGAAVPPDARLEGMDIFPILEGRAPILERTLFWRTAAGNHNQKAVRR
                     GDYKLLIDANHEFVFDVRRDLGERTDLAAERQELARTLQQQLAAWEADVDAEARANGT
                     ASFNAGRGVRAGGRGGPGRGQ"
     gene            1602..>2153
                     /locus_tag="VFC01_26120"
     CDS             1602..>2153
                     /locus_tag="VFC01_26120"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN83127.1"
                     /translation="MTNRRQFLGTLAAAGAASLVPSSGLFGQGRVAKLNVRGGAIDVH
                     HHFVPPGGATNRPWSPQMSLEQMDKFGIGVAILSMTQNGELLYNGTEKGRAAVRAGNE
                     YGAKLMQQYPKKFGLMGGIPMPDLEGSLREVEYAYDTLKVDAIGIYTNDNHGRWPGDS
                     YFEPLWQELNRRNAIVYMHPLAPQ"
BASE COUNT          400 a          709 c          718 g          326 t
ORIGIN      
        1 aggactcgct gttcaacatc agcgaaggcg tgcacaacta cttgtccgcg aacaaggtcc
       61 cacacatctg gcacatcgac gtgggtggtg cgcatacctt cccggtctgg aagaacgatc
      121 tctatcacct ctcgacattg ctctttcggt agccgtcgga ggagaacgcc atgcagatgc
      181 tgacggtggc cggtctggcg atggccgtcg tgtggctggc cggcgtcgcg ccccgcgcac
      241 agtcgcaagt gccgtcgccc gcagggcgac cgaacgtcgt cctcatcatc acggatgacg
      301 tcggctatgg cgacatcggc agctacggcg ccccggacat caagacgccg aacatcgacg
      361 gcctggctcg gggcggggtg cgcctgaccg atttctacgc caacgccaca ctgtgcagcc
      421 cgactcgggc cggtctcgtg acgggtcggt atcagcagcg cttcgcgatc gaggccgcgc
      481 ttggcggggt gagcacggcg gaagaacagg ggctgccggc cagcgggcgg tcgctgccgc
      541 aactgctcaa gaccagcggg tacgccaccg cgctcctcgg gaagtggcac ctgggctaca
      601 agccagagtt cagcccgaac gcccacggct acgactactt cttcggcttc aagagcggct
      661 acatcgacta ttaccagcac accgacggcg cgggcctgcc cgacctcttc gagaacgccg
      721 cgccggtaca gcaggcgggc tatatgaccg acctgatcac tgccaagacc gtgcgcttca
      781 tcgagcagaa cgcggggaag ccgttcttcg ccgaggtggc ctacaacgcc gggcactggc
      841 cgtatcaggt gccagacaag ccgtcggtgg ccatcggcaa cggccgccac ctgatgccgc
      901 acgacgccac gagcacgcgc ggcgactacg tggcgatgat ggagcgcgcg gaccagggtg
      961 tcggagagat tctgcggacg ctggaccgtc tgaacctggc ccgcaacacc atcgtgatct
     1021 tcaccaacga caacgggggt gaatggctgt cgcgcaacgc gccgctcttc aaccgcaagt
     1081 ggactctctg ggagggcggc attcgcgtgc cggccatcgt caggtggccg ggacgcattc
     1141 ccgccggccg cgtctccccg caggtgggca tcaccatgga cttcacggtt tcaatcctgg
     1201 ctgcggcggg cgccgccgtg ccgcccgacg ccaggctcga agggatggac atctttccga
     1261 tcctcgaggg acgagcaccc atcctcgaac gaaccctctt ctggcgaaca gccgcgggga
     1321 accacaacca gaaagccgtg cggcgcggcg actacaagct cctcatcgac gccaatcacg
     1381 agttcgtgtt cgacgtacga cgggatctcg gcgagcggac ggacctggcg gctgaacgac
     1441 aggagctggc ccggacgctc cagcaacagc tggccgcgtg ggaggccgac gtggacgcgg
     1501 aggcgcgggc caacggcacg gcgtcgttca acgccggccg gggagtccgc gcgggtggtc
     1561 ggggcgggcc aggacgaggc cagtagcgaa cagaggtgcc catgaccaac agacgccagt
     1621 tccttggaac gctggccgcg gccggtgcgg catcgctggt tccgtcgtcc ggcctattcg
     1681 gccagggtcg ggtggcgaag ctgaatgtcc gcggcggcgc gattgacgtc catcaccact
     1741 tcgtgccgcc cggcggcgcc acgaaccggc cgtggtcgcc gcaaatgtcg ctcgaacaga
     1801 tggacaagtt cggcatcggc gtcgccatct tgtcgatgac gcagaacggt gagctgctct
     1861 acaacggcac cgagaagggg cgggccgcgg tgcgggccgg taacgagtac ggcgccaagt
     1921 tgatgcagca gtaccccaag aagttcggcc tgatgggcgg catccccatg cccgacctcg
     1981 aaggctcgct cagagaagtg gagtacgcct acgacacgtt gaaggtcgat gcgatcggta
     2041 tctacacgaa cgataaccac gggcggtggc ccggcgactc ctatttcgag ccgctgtggc
     2101 aggagctgaa ccggcgcaac gccatcgtct acatgcaccc gctggcgccg cag
//
LOCUS       DASRJA010000588         5340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2288071,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000588 DASRJA010000000
VERSION     DASRJA010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2288071"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..241)
                     /locus_tag="VFC01_26125"
     CDS             complement(101..241)
                     /locus_tag="VFC01_26125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83128.1"
                     /translation="MGDATASSSFSNAALAFTGGTADASGKGIGNLAIAGTDSPATHR
                     IY"
     gene            complement(1032..1394)
                     /locus_tag="VFC01_26130"
     CDS             complement(1032..1394)
                     /locus_tag="VFC01_26130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83129.1"
                     /translation="MTTTERPPLPPVVCAPWCEHGHGHANEMFRGDQTCWGPSAYVDL
                     SLEDIARDDRGVYPQKVGVMAYRHWREGPCVYVHLEDIELSNSGTIDHCLHLTSDEAR
                     ALAAGLLRVADQIEGGTG"
     gene            complement(1758..1937)
                     /locus_tag="VFC01_26135"
                     /pseudo
     CDS             complement(1758..1937)
                     /locus_tag="VFC01_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
     gene            2267..2572
                     /gene="rpsJ"
                     /locus_tag="VFC01_26140"
     CDS             2267..2572
                     /gene="rpsJ"
                     /locus_tag="VFC01_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HZN83130.1"
                     /translation="MAGQKIRIRLKAYDHEAIDASARKIVETVTRTGASVVGPVPLPT
                     EKNVYCVIRSPHKYKDSREHFEMRTHKRLIDILDPTPKTVDALMRIDLPASVDVNIQ"
     gene            2587..3240
                     /gene="rplC"
                     /locus_tag="VFC01_26145"
     CDS             2587..3240
                     /gene="rplC"
                     /locus_tag="VFC01_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HZN83131.1"
                     /translation="MARKGILGTKLGMTQVFDENNRVVPVTVVKAGPNVVTRIRTPER
                     DGYSAVQLAYGEISPRKVNKPLTGQYKAANVNPRRHLAELRLDDESAASEYEVGQELT
                     AEIFADGTYVDVTGTSKGKGFAGTMKRHGFSGQGASHGAQAVHRRPGSIGGCATPGRV
                     FKGTRMSGRMGNDRVTTQNLLVHKVDAENGVLLIKGAIPGRTGGLVVVRSAIKRGEK"
     gene            3241..3903
                     /gene="rplD"
                     /locus_tag="VFC01_26150"
     CDS             3241..3903
                     /gene="rplD"
                     /locus_tag="VFC01_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZN83132.1"
                     /translation="MAAKTLKIDVHTPGGKTDGSVELPAELFDVEPNIALMHQVVEAQ
                     RAAARQGTHSTKTRGEVRGGGKKPYRQKGTGRARQGSTRAPQFTGGGTVHGPKPRDYS
                     QRTPKKMIAAALRGALSDRARNGRIHAVTELVEGQTPSTKSAKAFLATLTEHKQVLIV
                     IGRSDETGALSARNLPGVHIISPDQLNTYDVLKADDVVFSVEALNAYISANSKTAEEV
                     SA"
     gene            3903..4205
                     /gene="rplW"
                     /locus_tag="VFC01_26155"
     CDS             3903..4205
                     /gene="rplW"
                     /locus_tag="VFC01_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZN83133.1"
                     /translation="MATVTDPRDIILSPVISEKSYGLIEDNVYTFVVHPDSNKTQIKI
                     AIEKIFKVKVASVNTANRQGKRKRTRAGYGKRKDTKRAIVTLAAGSKPIDLFGAPA"
     gene            4237..5079
                     /gene="rplB"
                     /locus_tag="VFC01_26160"
     CDS             4237..5079
                     /gene="rplB"
                     /locus_tag="VFC01_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZN83134.1"
                     /translation="MGIRKYKPTTPGRRGASVSDFAEITRDHPEKSLVRPLHGKGGRN
                     AHGRITTRHKGGGHKRAYRVIDFRRHDKDGVNAKVAHIEYDPNRTANIALLHYLDGEK
                     RYIIAPEGLSQGDVVESGANADIKPGNNLPLRNIPAGTVIHAVELRPGGGAKLARSAG
                     AGIQLLGKEGTYASLRMPSGEIRRVDVRCRATVGEVGNAEQANINWGKAGRMRWKGKR
                     PTVRGVVMNPVDHPHGGGEGKTSGGRHPVSPWGKPEGRTRKAHKPSDKLIVRRRRTGK
                     KHHR"
     gene            5089..>5340
                     /gene="rpsS"
                     /locus_tag="VFC01_26165"
     CDS             5089..>5340
                     /gene="rpsS"
                     /locus_tag="VFC01_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZN83135.1"
                     /translation="MPRSLKKGPFVDDHLLKKVDVQNEKNSKQVIKTWSRRSTIIPDF
                     IGHTFAVHDGRKHVPVFVTEAMVGHKLGEFAPTRTFKGHI"
BASE COUNT         1047 a         1718 c         1745 g          830 t
ORIGIN      
        1 gaaggcagga gattagtggt ccagacccgt tgatcagggc cactcagccc gtgtggggtg
       61 cttcctgatc gatgtcgtct ccctctccat cgcgtcatcc tcaataaatt cgatgtgtgg
      121 ccgggctgtc ggtgcccgcg atggcgaggt tgccgatccc cttgccgctg gcgtcggcgg
      181 tgccgccggt gaacgccagg gcggcgttgc tgaagctcga actggccgtg gcgtcaccca
      241 cgccgaccga gagtccactg accgagaccg cgcaccaggg tcggccgatg cggtgggtgc
      301 cccgatgtcc agcgccaaga ggggagcgcc atcacacccg gcgcacgcag cggcaaactt
      361 gaccgatgtc ctcatgagac ccctcctcaa ttcttatccg ccaaagccgc cgacgtcgca
      421 aacgttagat ctgtttgctg tccgcgatag caaattcgcc gaaaaagtcg ggccatcggg
      481 ccccatgggt cggtgcccgc ccgatacgtg ctcagtaggt ggatcaggct gcggttctcg
      541 ccgtcgatgc gcagcgtcca gccgaccagg tagccgaggc cgacaaatcg ccaggttcgt
      601 cagaggtgag cggatcgggc gcgcgcaggc ttgtccagcc cttaccccac gcgatagcga
      661 ccgcatagag atgcgtggtg cccaccagga tcttgagatc gggcgttgcc gtgatgtcgt
      721 cgattaggta cggggacgag tacttggaac ggttcgccgg gtgatgggaa cagcgccacg
      781 gtgaccggtg actgggcttt agcagttgct cactttggcg atgagaacaa gccacggcta
      841 caggttacca acgcgccgca tttgcaggcc cacagggata gcaactcggc caccgtcaac
      901 ggcgactaca acgacgcgta tgcgtacctg agggtggcaa acaccgccac ggtcaccggc
      961 gagtggggct gtaacgggcg acccgtttcg ggcttggcgg gacgaaccaa tagatcagac
     1021 gggggatggc gttatccggt tccgccctcg atttgatcgg cgacgcgaag caatccagcg
     1081 gccagcgccc gcgcctcatc gctcgtcagg tgcaggcagt gatcgatagt cccgctgttg
     1141 gacagctcga tgtcctcgag atggacatac acgcaagggc cttcccgcca atgccggtag
     1201 gccatcaccc cgaccttctg cgggtacaca ccgcggtcgt cgcgcgcgat gtcctcaagc
     1261 gacaaatcga cgtacgcgga tggcccccaa catgtttgat cgccccggaa catttcgttg
     1321 gcgtggccgt ggccgtgctc gcaccacggt gcacatacga cgggcggaag cggcggacgt
     1381 tcggtagtgg tcatcgcccg gcacatctgt cgatctcgtc ggcacactcg agaagcaccg
     1441 aagcgagttc acgagcctga gaacttgtga ggcccgtgtc agagttggtc tccgcgtaga
     1501 cgaacggatg ttcgttgagg ctgccgtcgt ccacccgacc gtctgggtac tggaccgcgg
     1561 tcgtcgcaac gatcacgtcg tcgcggccag caatgccacg gctagtcccg tgcaggatgc
     1621 ggtagggctc gtcgtcgtct taacagatgt caacttcggc gccggccggg tgcaggacgt
     1681 tggcgaggtt gtcggttgtg gtggtcattt tggtgatgtc ccctatctcg ctgtttgcta
     1741 catcacccgc atgtgctgac gaagaaccac ttgggcacgg tgaccagggc gaaaccgggc
     1801 tccatccgca ccacatgcgg cacgatcgac gcgaaatacg gcactcgcgc catcgcggcg
     1861 gccgagaaca gccgggtgcc gccgggcact gaatcgaggc gtttccacat ccgatatgta
     1921 gtggtctctg ctgccatggc gggctacctt actctggagt aaggtagctg cggattctcc
     1981 gtggccagga atttggatga ggccccacat ctagggcata ctattcgggt tgccttgagc
     2041 cgggttcgcg cctggccggg gcatacgacc agcgcccgaa cgggcgcgtc cggtctccac
     2101 ctcgatcgtg acgattttcg ccgcggccga gtgtgcgtaa gggcgacacg cccgaccgcg
     2161 gggatcggtg gaccacagac agataaacag cggcggcata ccagcgcgag ttgcgccgga
     2221 cagagcggcc cagacatggg cccagtagga gtgaggtagg agaagcgtgg cgggacagaa
     2281 gatccgcatc aggctcaagg cctacgacca cgaggcgatt gacgcctcgg cacgcaagat
     2341 cgtggagacg gtcacccgta ccggcgccag cgtggttggc cctgtgccgc tgccgactga
     2401 gaagaacgtg tactgcgtca tccggtcccc gcataagtac aaggactcgc gggagcactt
     2461 cgagatgcgg acgcacaagc gactgatcga catcctcgac ccgacgccga agaccgttga
     2521 cgccctgatg cgcatcgatc tgccggccag tgtcgacgtc aacatccagt aggagaccag
     2581 agaacgatgg ctagaaaagg cattctgggc accaagctgg gcatgacgca ggtgttcgac
     2641 gagaacaacc gggtcgttcc ggtgacggtc gtcaaggccg gccccaatgt cgtgactcgg
     2701 atccgtacgc cagagcgtga cggctactcc gccgtgcagc tcgcctacgg cgagatcagc
     2761 ccgcgcaagg tgaacaagcc gctgaccggg cagtacaagg cggccaacgt caacccgcgg
     2821 cgccacctcg ccgagctgcg cctcgacgac gagtcggccg caagcgaata cgaggtcggc
     2881 caggagctga ccgccgagat cttcgccgac ggcacctacg tcgacgtgac gggcaccagc
     2941 aagggcaagg gtttcgccgg caccatgaag cgccacggct tttccggcca gggcgccagc
     3001 cacggtgcac aggcggtgca ccgtcgtccc ggctccatcg gtggttgtgc cacccccggc
     3061 cgggtgttca agggcacgcg catgtcgggc cggatgggca acgaccgcgt gacgacgcag
     3121 aacctgttgg tgcacaaggt cgatgccgag aacggcgtgc tgctgatcaa gggtgccatc
     3181 cccggtcgca ccggtggact ggtcgtggtc cgcagcgcaa tcaagcgagg cgaaaagtaa
     3241 atggctgcca agactctcaa gattgatgtc cacaccccgg gtggcaagac ggacggctct
     3301 gtcgagctgc ctgccgagct gttcgacgtc gagcccaaca tcgcgctgat gcatcaggtc
     3361 gttgaggcgc agcgtgccgc ggcccgccag ggcacgcact cgacgaagac gcgcggcgag
     3421 gttcgcggcg gcggcaagaa gccgtaccgc cagaagggca ccggccgtgc ccgccagggt
     3481 tcgacgcgtg ccccgcagtt caccggtggt ggcaccgtgc acggcccgaa gccgcgcgac
     3541 tacagccagc gcacgcccaa gaagatgatc gccgccgccc tgcgcggggc gctgtcggac
     3601 cgggcgcgca acggtcgcat ccacgcggtc accgagctgg tggagggtca gactccgtcg
     3661 accaagagcg ccaaggcgtt cctggccacg ctgaccgaac acaagcaggt gctgatcgtg
     3721 atcggccgca gcgacgagac cggtgcgctg agcgcgcgca accttccggg tgtgcacatc
     3781 atctcgccgg accagctcaa cacatatgac gtgctgaagg ccgacgacgt ggtgttcagc
     3841 gtcgaggcgc tgaatgccta catcagcgcg aattccaaga ccgccgagga ggtttcggcc
     3901 tgatggcgac cgtcactgac ccccgcgaca tcatcttgtc cccggtcatc tcggagaagt
     3961 cctacgggtt gatcgaggac aacgtgtaca cgttcgtcgt gcaccccgac tcgaacaaga
     4021 cgcagatcaa gatcgcgatc gagaagatct tcaaggtcaa ggtcgcgtcg gtgaacaccg
     4081 ccaaccggca gggcaagcgc aagcgcaccc gcgccggcta cggcaagcgc aaggacacca
     4141 agcgcgccat cgtcacgctg gccgcgggaa gcaagccgat cgacctgttc ggagcaccgg
     4201 cctaggccgg cgggaagcag agagacctag agaatcatgg gaattcgcaa gtacaagccg
     4261 acgaccccgg gtcgtcgcgg tgccagcgtc tccgatttcg ccgagatcac tcgcgaccat
     4321 ccggagaagt cgctggttcg tccgctgcat ggtaagggcg gccgcaacgc gcacggtcgg
     4381 atcaccaccc gacacaaggg cggtggccac aagcgtgcgt accgcgtgat cgacttccgt
     4441 cgccacgaca aggacggcgt caacgccaag gtcgcgcaca tcgagtacga cccgaaccgc
     4501 accgcgaaca tcgcgctgct gcactacctc gacggcgaaa agcgctacat cattgccccc
     4561 gagggacttt cgcagggcga cgtggtggag tcgggtgcca acgccgacat caagcccggc
     4621 aacaacctgc cgctgcgcaa catcccggcg ggcaccgtga tccacgccgt ggagctgcgg
     4681 cccggtggcg gcgccaagct ggcccgctcg gccggcgccg gtattcagct gctcggcaag
     4741 gaaggtacct acgcgtcgct gcgtatgccg tccggcgaga tccgtcgcgt cgacgtgcgc
     4801 tgccgcgcca cggtcggcga ggtcggcaac gccgagcagg ccaacatcaa ctggggtaag
     4861 gccggccgga tgcggtggaa gggcaagcgc ccgaccgtcc gcggtgtcgt gatgaacccg
     4921 gtcgaccacc cgcacggcgg cggcgagggc aagacctccg gcggccgtca tccggtcagc
     4981 ccatggggta agcccgaggg ccgtacccgc aaggcgcaca agccaagcga caagctcatc
     5041 gtccgacgcc ggcgcaccgg caagaagcac caccggtagg agtagccgat gccacgcagc
     5101 ctgaagaagg gcccgttcgt cgacgaccat ctgctcaaga aggtcgacgt ccagaacgag
     5161 aagaacagca agcaggtcat caagacctgg tctcgtcggt cgaccatcat ccccgacttc
     5221 atcgggcaca ccttcgcggt gcacgacggc cgcaagcatg tcccggtgtt cgtcaccgag
     5281 gcgatggtcg ggcacaagct cggcgagttc gcgccgaccc gcacgttcaa gggtcacatc
//
LOCUS       DASRJA010000589         3704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3625800,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000589 DASRJA010000000
VERSION     DASRJA010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3625800"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFC01_26170"
     CDS             <1..406
                     /locus_tag="VFC01_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN83136.1"
                     /translation="SNERGMSLRSPARFLAPAERLVEQWKANPDPVFADRVADTWIKA
                     QAYRLHTFGTVTRVAAGGELGAESSVTKVFWSELDVALHQTALDIRGADGELADAWTD
                     GLLFALGGPIYAGTNEIQRNIIAERLLGLPRK"
     gene            410..1363
                     /locus_tag="VFC01_26175"
     CDS             410..1363
                     /locus_tag="VFC01_26175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN83137.1"
                     /translation="MKFDLDEQQRDFAASIDAALAAADVPAAVRAWAAGDTSPGRKMW
                     AQLADLGVTALAVPEKYDGIEAHPVDLVVAAERLGRWGVPGPVTESIAVAPVLLADDE
                     RSAALATGELIATVALPPQVPRAVDADSAGLILVAADGSVRDGVAGEQHESVDPSRKL
                     FDVTASGDPQPADVARAYEFGALATAAQLVGAGQALLDTSVEYVKQRRQFGKVIGTYQ
                     AIKHKLADVHIAVELALPLVYGAALSLADNSPDTARDVSAAKAAASDAALLAARSALQ
                     VHGAIGFTQEHDMSLLLLRVQALHSAWGDPTVHRRRVLEAL"
     gene            1395..2336
                     /locus_tag="VFC01_26180"
     CDS             1395..2336
                     /locus_tag="VFC01_26180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN83138.1"
                     /translation="MTEERELLRETVAALVDKHASPAAVREAMASERGYDESLWKLLC
                     EQVGAAALVVPEELGGAGGELADAAVVLEELGKALVPTPLLGTTLAELALLAADEPDA
                     ETIERLAEGTSIGTVVFDPGYVVNGDVADVMIAADGQRVSRWTTVTSHRVDTMDLTRR
                     LARVEPTGIKDIGADPGLADAAAILLAAEQIGAAAKCLELTVEYTKDRVQFGRPIGSF
                     QALKHRMADLYVAVQSARAVVNDAIAEPSATSAALARVAASEAFSKVAAEAVQMHGGI
                     AITWEHDIQLYFKRAHGSAQLLGPPREHLRRLESEVF"
     gene            complement(2333..3151)
                     /locus_tag="VFC01_26185"
     CDS             complement(2333..3151)
                     /locus_tag="VFC01_26185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN83139.1"
                     /translation="MNDEHYAINRAWWDERAPAHASSSDYAVQELIADPARLSDVVRF
                     DLPRLGEVNGQRGIHLQCHIGTDTISLHRLGAAMTGLDFSSESIAIARRIATDARADV
                     NFVQADVYSARAVVGAGFDFVYTGIGALCWLPDVKRWAQTVTALLRVGGRLFLREFHP
                     MMWAVDETRTDALVLGYPYFEQPEPDTFDDPGTYVDTAIEFGHNESRSWNHGLGEIVA
                     AVLDAGLELTMLVEHDSVPSDALPGRMHRDDGEWRLIEHRERLPLSYTLQAVKR"
     gene            3213..>3704
                     /locus_tag="VFC01_26190"
     CDS             3213..>3704
                     /locus_tag="VFC01_26190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZN83140.1"
                     /translation="MSVALRAGIPPFYVMDVWLAAAERQRTYSDLVNLSAGQPSAGAP
                     TAVREAAKQALDSGPLGYSVALGIPELRTAIASSYADRHALAVDPNDVVITTGSSGGF
                     LLAFLACFDPGDRVAIASPGYPCYRNILSALSCEVVEMPCGPETRFQPTAPMLADLDP
                     PVQG"
BASE COUNT          619 a         1212 c         1252 g          621 t
ORIGIN      
        1 aagcaatgag cggggcatgt cgctgcgcag tcccgcacgc tttctggccc cagccgaacg
       61 gctcgttgag caatggaaag caaatccgga tccggtcttc gcggaccgag tggccgatac
      121 ctggatcaag gcgcaggctt accggctgca caccttcggc accgtgacac gggtggcggc
      181 gggcggtgag cttggcgccg aatcgtcggt gaccaaggtg ttctggtccg agctcgacgt
      241 cgcgctacac cagactgcgc tcgacatccg gggcgccgac ggtgagctgg ccgacgcgtg
      301 gaccgacggg ctgctgttcg cgctcggtgg cccaatctac gcaggcacca acgagattca
      361 gcgcaacatc attgctgagc gcctactggg acttccccga aagtagacca tgaaatttga
      421 tcttgacgaa cagcagcgcg acttcgccgc cagcatcgac gcggcgctgg ccgccgccga
      481 tgtgcctgcc gctgtgcgtg cctgggccgc aggagatacc tcccctggtc gcaagatgtg
      541 ggcacagctc gctgacctcg gcgtcaccgc actggcggta ccggagaaat acgacgggat
      601 cgaggcgcac cctgtcgatc tggtggtcgc agcggagcga ctgggccgtt ggggtgttcc
      661 cggcccggtc accgaatcca ttgcagtcgc accggttctg ctcgccgacg atgagcggtc
      721 agccgcactg gcgaccggtg agctgatcgc caccgtcgcg ctgccgccgc aggtgcctcg
      781 ggcagttgac gccgattcgg cggggctcat tctggttgcc gctgatggtt ccgtccgtga
      841 cggcgtggcg ggcgaacagc acgagtccgt cgacccgagc cggaagttgt tcgatgtcac
      901 cgcttccggt gatccgcagc ctgccgatgt cgcgcgcgcg tacgagttcg gtgcgctggc
      961 cacggccgcg cagctggtcg gcgcgggtca ggcgctgctg gatacctctg tcgaatatgt
     1021 caagcagcgc cgtcaattcg gcaaggtgat tggcacctat caggccatca agcacaaact
     1081 tgccgacgtg cacatcgccg tcgagctggc cctgccactg gtctacggcg cggcgctctc
     1141 gctcgccgac aattcaccgg acaccgcgcg tgatgtcagc gcggccaagg ccgccgcgtc
     1201 cgacgctgcg ttgctcgccg cgcgctcggc cttgcaggtt cacggcgcca tcggcttcac
     1261 gcaggaacac gacatgtcgc tgcttctact ccgagtgcag gcactgcatt cggcgtgggg
     1321 cgacccgact gtccatcgtc ggcgcgtcct ggaggcattg tgagcggcga gcgaggagcg
     1381 aaggcggatc gcgcatgacg gaagaacggg aactactgcg cgagaccgtc gcggcgttgg
     1441 tcgacaagca tgcgtcgccc gcggcggtgc gtgaggcgat ggcatccgaa cgcggctacg
     1501 acgagtcgtt gtggaagctg ttgtgcgagc aggtcggcgc ggccgcgctg gtggtgcccg
     1561 aagaactggg cggtgccggc ggcgaactcg ccgacgctgc agtggtgctc gaggagctgg
     1621 gcaaggcgtt ggttccgacg ccattgctcg gcaccacgct cgccgaactc gccttactgg
     1681 ctgcggatga gcccgacgcc gaaacaatcg aacgcctggc cgagggcacc tcgatcggca
     1741 ccgtcgtatt cgaccccgga tacgtggtca acggcgacgt cgccgatgtc atgatcgcag
     1801 cggacggtca gcgggtgagc cgatggacaa ccgttacctc gcaccgtgtc gacacgatgg
     1861 acctcacgcg ccgtctcgcg cgggtcgagc ccacgggaat caaggacatt ggcgccgacc
     1921 ccgggctcgc cgacgccgcc gcgatcctgc tggccgccga gcagatcggc gctgcggcga
     1981 agtgtctgga actgaccgtc gaatacacga aggatcgtgt gcagttcggt cgaccgatcg
     2041 gcagtttcca ggcactcaag caccggatgg ccgatctcta tgtcgcggtg caatcggcgc
     2101 gcgcggtcgt caacgacgcg atagccgaac cgtcggcgac gtccgcggca ctggcacggg
     2161 tggcagccag cgaggcgttt tcgaaggtgg ccgccgaagc cgtccagatg catggcggga
     2221 tcgcaatcac ttgggagcac gacatccagc tgtacttcaa gcgcgcacac ggcagcgcgc
     2281 agctgctcgg tcctccgcgg gaacacctgc gccgactcga atccgaagtg ttctaacgtt
     2341 tgacggcttg cagcgtatag ctcagcggga ggcgctcccg gtgctcgata agccgccact
     2401 caccgtcgtc ccgatgcatg cgcccgggaa gcgcgtccga cggcacggag tcgtgttcga
     2461 cgagcatggt gagctcgagg cccgcgtcga ggaccgcggc gacgatctcc ccaaggccat
     2521 ggttccagct gcgagattcg ttgtggccga actcgatcgc ggtgtcgacg tacgttccgg
     2581 gatcatcgaa ggtgtccggc tcgggttgct cgaagtacgg gtagcccagc actagtgcgt
     2641 ccgtccgggt ttcgtcgacg gcccacatca tcggatggaa ctcgcggagg aacagacggc
     2701 cgcccacacg caacagcgcg gtaacggtct gcgcccatcg cttcacgtca ggtagccagc
     2761 acagcgcgcc gatcccggtg taaacgaagt cgaatccggc gccaacgacg gcccgagcgc
     2821 tgtatacgtc cgcttggaca aagttaacgt cggcacgagc atcggtggct atacgccgag
     2881 cgatcgcgat cgattccgac gagaagtcga ggcctgtcat cgctgcgccg agacggtgca
     2941 gtgagatcgt gtcggtgccg atgtggcatt gcagatgtat tccgcgttgt ccgttgacct
     3001 cgccgaggcg gggtaggtcg aaccggacga cgtcggacag ccgcgccggg tcggcgatga
     3061 gttcttgaac cgcgtagtcc gacgaactcg cgtgcgcggg cgcgcgttcg tcccaccagg
     3121 ctctgttgat cgcgtagtgc tcgtcgttca tgggcgtatg cccgggaagg ctaccgtttc
     3181 gagcgagcag cccgcttgtt agcctcgctc caatgagcgt tgcgctgagg gccgggattc
     3241 caccgttcta cgtgatggac gtgtggctgg ccgccgcgga gcgccagcgc acgtacagcg
     3301 acctggtaaa cctgtccgcc gggcagccga gcgccggcgc acccacagcg gtgcgggagg
     3361 cagctaagca ggcgttggac agcggcccac tcggctactc ggtcgcactg ggcatccccg
     3421 agctgcgcac cgcgatcgcc agctcgtacg cggaccggca cgccctcgcc gtcgacccca
     3481 acgacgtcgt gatcaccacc gggtccagcg gcggtttcct gctggcgttc ctggcctgct
     3541 tcgaccccgg cgaccgcgtc gccatcgcca gccctggcta cccctgctac cgcaacatcc
     3601 tgtcggcgct gagctgcgag gtcgtcgaaa tgccgtgcgg gccagagacc cgattccagc
     3661 cgacggcccc gatgctggcc gacctggatc cgccggtcca gggc
//
LOCUS       DASRJA010000590        22192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2362389,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000590 DASRJA010000000
VERSION     DASRJA010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22192
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2362389"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VFC01_26195"
     CDS             complement(<1..1103)
                     /locus_tag="VFC01_26195"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZN83141.1"
                     /translation="MTIYEYDMVSAASECEALVPPVPSHDALALVDRLNAGEPYAVAF
                     GGQGSAWLENLEELVACAGIECELNEIVGEAAVLLEPVARELVVVRPIGFEPMEWVRA
                     LAAGESVPATKQLITAAISGPGILLAQMAAIRALTRQGLDLYDTPPVAMAGHSQGIIA
                     CESLRANGARDAELLALLQLIGAAGSLVSRRRGMVGRGDKSPMVSVTNVYPDRIAELL
                     DEFSKDVRTVLPPVLSIRNGRRSVVITGTPEQLGRFELYCSKITEKEEAERKNKLRGG
                     AVFNPVFSGIQVEVGFHTPRLADAVDLIDTWAARTPGIDAELTHQFAQSVFVEPVDWV
                     AEVERLYDAGAKWIVDLGPSDTATRVTAPVVRGL"
     gene            1936..3177
                     /locus_tag="VFC01_26200"
     CDS             1936..3177
                     /locus_tag="VFC01_26200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014981078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HZN83142.1"
                     /translation="MRILALGAAGEIGRTAARVLAGDDRITQLVVADRNARAATRVAR
                     ALGAKGVPLALDVTDRPALDAAMRDCDFVINTVGPFFLFGPPILSAAIDAGRDYIDIC
                     DDPEPTLEMLAFDDRAKAAGVTALLGMGASPGVANLLAVIAGRELETVDTVITAWNIN
                     AAQSDPARTGATSAAIVHGMRQISGTIPVTRARGLEHRPALARIRVSYPGIGAVRGRS
                     FGHPEGVTLSRAFPGLRESTNMVVGDRLTLAVVSVLRWVINQRIMTIDRAAYVAARLG
                     RLMVDKPAHILTSGGAPPLFALATGTRQDSTCTVATALAQLPGLSMAANTAVPLAVAI
                     PLIAASRRPGVHTPETLLDPAAFFSAFARHCIGDPAPAAMTITTRSWASPEDNAAALN
                     ASLLTALLNARCRTHIADALI"
     gene            complement(3256..5613)
                     /locus_tag="VFC01_26205"
     CDS             complement(3256..5613)
                     /locus_tag="VFC01_26205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012834421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN83143.1"
                     /translation="MTVVPDLTAKATPTNIDETVEPPSTARRVSKDRGVQGRPGVLYR
                     WAVLMATHWRTVLVVWAVVVSACIIAMPAVEKSLKAPDFNVTTAESTEAARILDRDFP
                     SVGAEQNVVVFDFRQHDIDAPASQETILRALSEVRSVPGVAKVVSPFDRISMPLISGD
                     GGTAIALVGLVGNQSSRSDAAAEVADKLARLSSDAVWMGITGPSPMQTDQIRVSVADT
                     QRAEMIGLPVALLLLIFSLGTLVSAALPIVVALAGIAVAYGLVFGMTGLVSFDTLVLA
                     VTTMIGTGVGIDYSMFIVSRFREELAHSDIRDPAARRVAAVAAAADTAGRTILVSGVI
                     VMVSLGALIVVQMPLFRAIAVGVAAAVVAALLVSLTLLPALLAGLGSKINFGSLPARF
                     LPADSQAGHIVGGWQRWAKLVMRRPVMFATAAVAVLVLAALPLTGVKFGLDAGMQALA
                     GEPSGHANQLIEAKFTSGLLAPVQAIMTGSDRGPMTDSQAHQAGAFVGRLSEDHRVSY
                     VLQEVDNGRMVATVILNVPIDSDAATELVRSVRADAGKLAGDGGPTVHIGGATAQYVD
                     ISEEISKKSPLVFGLVLAFSFVFLVIAFRSILLPIKAILMNLLAIAATMGVTVAVFQW
                     GTGESLFDFRSPGFIQVYLPTVIFAVLFGLSMDYEVFLIGRIREYWDRGESNEDAVAS
                     GLAHTARPITTAAAIMVAVFASFVTAHVLEHKQIGFGLALAVAIDALIVRLILVPALM
                     RTFGRWNWWLPKIGTKTNRASNNSEISCDSTTNGTSLRLPAHSST"
     gene            6024..6272
                     /locus_tag="VFC01_26210"
                     /pseudo
     CDS             6024..6272
                     /locus_tag="VFC01_26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612219.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6514..7842
                     /locus_tag="VFC01_26215"
     CDS             6514..7842
                     /locus_tag="VFC01_26215"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN83144.1"
                     /translation="MAVEADSSGSGQPRQQTRWSYRGPPQSAVSSPARNAHYAERIAR
                     QRRVVNILTWLAAAFSASFAVLLLVAQRGSIYMAIVNAVMALVFATIPVLHRFGSLVA
                     PLTLILTTFVSLSIVCWDIGTGSGLDLFFVVVAAAAVLVLGIEHIALASILVAIGAAL
                     VITLQFTVPPDTGVQPPWFVAFGFITSVICACIMSVAAVWYALREIERAEEAMELEYQ
                     RSESLLANILPASIASRLKTQPTSVIADRYADASVLFADIVGYTDWASQTAPAQLVQF
                     LNRLYTDLDRLVDDHGLEKIKTSGDCYIVVSGVPERREDHLQALARLALSMAAAVDGL
                     SDPRGRRLRLRIGLGAGPVAAGVVGIRRFFYDVWGDAVNVASRMESTGVEGKIQVPEV
                     VHGRLKDHFVLEERGEVVVKGKGVMRTWFLIGERGRPTEADARSNSAEAP"
     gene            complement(7951..8190)
                     /locus_tag="VFC01_26220"
     CDS             complement(7951..8190)
                     /locus_tag="VFC01_26220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83145.1"
                     /translation="MNARQAWALLATGVLSYELYCKDGELLSECVDEWLDCRPLLTRA
                     VIAALALHLGNLLPARYDVVSLGFFAMRKAKRYWR"
     gene            complement(8884..9603)
                     /locus_tag="VFC01_26225"
     CDS             complement(8884..9603)
                     /locus_tag="VFC01_26225"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HZN83146.1"
                     /translation="MDGETHTVPEFRVDLIDDFCLTVRDTVVPLPLGCQRLVALVALA
                     DRPIRRSRIAATLWPDTSEERARANLRSALWRVSAMADALVVSAWHVGLADSVIVDYR
                     AEVAAFNRLHDARHDIAHDLALAREIGGRCGELLPDWSDEWVMVERERFRQIRLQTID
                     ALGERLCESGRYRDALRVVLAGLAMDPLRESPHRLMVKAHLRLGNVADAIRQYRTYER
                     LLDEELGVQPTREMTAMLAAG"
     gene            9895..10071
                     /locus_tag="VFC01_26230"
     CDS             9895..10071
                     /locus_tag="VFC01_26230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83147.1"
                     /translation="MWRKPVLLFPGQGTCRGMYATALECRVEMFTDDMELEADLGVDS
                     VKKTGPRLDPRHQK"
     gene            10082..11764
                     /locus_tag="VFC01_26235"
     CDS             10082..11764
                     /locus_tag="VFC01_26235"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HZN83148.1"
                     /translation="MNRPGAADDRRRTEGSAPRLHIAGPADALAMGIETVSERSSPSR
                     PNSLREATAELQARKELATRGAEQATQRQRARGKMTVRERLEALLDEGSFTETGMLAR
                     HRASGFGLERHRPDTDGVVTGWGTIDDRKVFVYAHDFRIFGGSLGEVFASKIHTLMDL
                     AAATGAPIIGLNDGGGARIQEGITALAGFGGIFARNVSLSGVVPQISVILGPCAGGAA
                     YSPALTDFVFAVKDKTNLFVTGPDVVEAVTGEKTTHEELGGAVTHATRTGVASFLIDD
                     ESGCFDEVRYLLSFLPSNNHEDPPSVVPTDRPSRRCDELVEIVPAEPNRAYDVREVIE
                     VIVDDGEYLEVQPLWATNIVCAFARLDGHVVGIVANQPSVLAGALNIDAAEKAARFVR
                     TCDAFNIPLVTLVDVPGFLPGVDQEHAGIIRRGAKLLYAYCESTVPRVQVILRKAYGG
                     AYIVMDSKSLGADLSFAWPSNEIAVMGAEGAASIIFRKELADAPDPVQHRRKLVADYT
                     EQLMTPYIAAERGMVDDVIDPGETRTILIRSLDMLRSKRASSPRRKHGNIPL"
     gene            11761..12048
                     /locus_tag="VFC01_26240"
     CDS             11761..12048
                     /locus_tag="VFC01_26240"
                     /inference="COORDINATES: protein motif:HMM:NF025192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004658 - propionyl-CoA carboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase epsilon subunit"
                     /protein_id="HZN83149.1"
                     /translation="MKPAMFQITKGDPTPEEIAALVAVLALASATTQPGSIGSTAARW
                     RGSAPPAEFNSAALGLRDRCWRPWGARWSRSGPAHAPAEHRIHWAGTRDAS"
     gene            complement(12189..12464)
                     /locus_tag="VFC01_26245"
     CDS             complement(12189..12464)
                     /locus_tag="VFC01_26245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83150.1"
                     /translation="MMPLTPDAHIDELRRVIGESEQRRQAAEIKLADRERRLGTARQT
                     IAAQDRRIGWLMAEVARLRDAAAWTMPDDYAREARRFFNVYSISGAR"
     gene            complement(12578..13150)
                     /locus_tag="VFC01_26250"
     CDS             complement(12578..13150)
                     /locus_tag="VFC01_26250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN83151.1"
                     /translation="MVDQDTAQLVRRAAAGDELAWRQLTDRLGPLLRRVSSAYRLGEA
                     EAADAVATVWLKLVENIGSLRDGNAVTGWLITTLRRECVARARARSRERPVADFAGVD
                     EPLDVVDFDRWLLDADRRQMVHRALMKLSEQQRQLITMLFAHPAPSYAEISASLAMPI
                     GSIGPTRCRLLGKLRDAMSEVDGRELAYAG"
     gene            14385..15092
                     /locus_tag="VFC01_26255"
     CDS             14385..15092
                     /locus_tag="VFC01_26255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN83152.1"
                     /translation="MSNDEPRGKDSINMSGRISVLIADDCLLVRDSVCRALSIDADVT
                     VVGVAEDYDSTVELVEKHKPTILVTDVRMPPTLTDEGIRLARWLRRAHPDIGVIVLSQ
                     YADPRYAAGLLDGGTSGRGYLLKQRISHFDELSEAVHQVAAGGTVLDPMVVEALLAQP
                     RSTSTGRLTSREREVLGELATGLSNRTVAQRLVLSQGAVEKHINSIFAKLGLTMDDSV
                     DRRVKAVLMFLDERASA"
     gene            complement(15183..15593)
                     /locus_tag="VFC01_26260"
     CDS             complement(15183..15593)
                     /locus_tag="VFC01_26260"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN83153.1"
                     /translation="MDVNMRITPRKVRSEGVAASATLVLNRLYREGPMRLTALALAEG
                     ASQSGMTQLVQRMERQGLLERWSDPDDGRASLVMLGESGRHMWDARADARRQRIAEFA
                     RGVPRRSAGIVACRPGSSARSRPDARDCRLASTG"
     gene            complement(15897..16271)
                     /locus_tag="VFC01_26265"
     CDS             complement(15897..16271)
                     /locus_tag="VFC01_26265"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HZN83154.1"
                     /translation="MWCGWLTEADPDLTRYHDLEWGTPVHDEAALFEELVSTYFENGL
                     SWRVVFSKREALRRAFADFDPAVVAGMTDRDVDLLMSDRTIIRNRAKIEATVHNARLL
                     TSISLNDIVWHYRHRRRRPLRR"
     gene            16300..16800
                     /locus_tag="VFC01_26270"
     CDS             16300..16800
                     /locus_tag="VFC01_26270"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZN83155.1"
                     /translation="MSRGDLEMANARLYPFWGSLTEALRTGQPQSEAKTGGDFFAAVY
                     ADPKRLAQFAAAMSGLSASAGEAIAAHFPWRDYGSVVDIGCAEGAVSAAIALAHEHVT
                     GGGFDLPPLEPLFNAYVASTGCWPSSTSPNPMTAKTQGNTTIRRQPLPNLQSAPKMTA
                     WPTLAR"
     gene            17053..18603
                     /locus_tag="VFC01_26275"
     CDS             17053..18603
                     /locus_tag="VFC01_26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007537009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HZN83156.1"
                     /translation="MSRISCPVLSAKIATAEESMRYIHHGDNVGFSGFTGAGYPKAVP
                     AALAAAMVQAHEDGREFQVGLWTGASTAPDADGVLAEAHGISSRLPYNSDPTLRRAIN
                     AGDVRYIDAHLSHSAQHMWFGFYGKLDVAVVEVTAALPDGLLVPSSSVGNNKTWLDQA
                     DRVILEVNHWQPRELEGFHDIYYGTALPPHRRPIMLIEALQRIGEPYLHVDRDKVVAV
                     VETHSPDRNAPFSPPDQASQAIAGHLVDFLRHDVRAGRMPPGLLPIQSGVGNVANAVL
                     AGLGDSEFTDLVAYTEVLQDGMLDLLDSGTLRAASATSFGLSSDGVRRFMTGIDGYKG
                     RILLRSQEISNHPELVRRLGVLAMNGMIEADIYGNVNSTHVMGSAIMNGIGGSGDFAR
                     NAFCSFFLSPSTAKGGAISAIVPMVSHVDHTEHDVDVVVTEQGLADLRGLAPRDRAPR
                     IIQRCAHPSFRDQLRDYFDRAFAARPNARHTPHLLDEALSWHQRYLDSGSMLPALQDG
                     APSVRATL"
     gene            complement(18731..18976)
                     /locus_tag="VFC01_26280"
     CDS             complement(18731..18976)
                     /locus_tag="VFC01_26280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83157.1"
                     /translation="MAPLVYAVADTESAAVLSRYKNHLVRTGRDTCATTIAYLLRQAI
                     LLHEQCFGAETGLPISLRTVIPSLTSRPGVHPLTSIA"
     gene            19779..19958
                     /locus_tag="VFC01_26285"
     CDS             19779..19958
                     /locus_tag="VFC01_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN83158.1"
                     /translation="MEDKAFNELPLLLAVPRAAQLLGISRASAYRLAASGELPVRRLG
                     GRVYVVTAGLRELSS"
     gene            20205..21476
                     /locus_tag="VFC01_26290"
     CDS             20205..21476
                     /locus_tag="VFC01_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014360696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZN83159.1"
                     /translation="MSTDTAPAEQPRKRGERTDSSIRKLPNGKGWRARPTLGTDPVTG
                     KQVRPSKVFATKKAAQGWVTEQRQQWSTATWVPKSSRTFDEVADHWLTLREADMSIGP
                     NTVRADRESLAYARRAFGAVPVQKLTPAALADWSATLTGKDSRPLAPATKRRAIVRLK
                     SVMAHARKMRWLTYDPSADLASPEQKAVTATAADDIWTPDQMGKVLDHVSAHRLGGCF
                     ALTLLGLRREEVGGLRWSDIDLDAGTLRIRQARVDVNGRDTIVTTKTQRSARDLPLPP
                     HELAMIKAMRTFHLRERLAVGRPLAADDLLLSRTDGTRLPVRDYSREFAAQRRAAGLK
                     AIPLGKLRHSNISRMRAAGIAADVVAAWHGDTERMTQAVYGRVTDDRLTATSAGFLGR
                     GDRTELGQVSRFHSPIAKNPRQIAWDFLSWS"
     gene            complement(21995..>22192)
                     /locus_tag="VFC01_26295"
     CDS             complement(21995..>22192)
                     /locus_tag="VFC01_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN83160.1"
                     /translation="HVGFGYGPHLCLGAPLARMEAQAVLRELVDRVSRIQTTGPTTWS
                     TNSSLRGPIQLPVLLHEGSTP"
BASE COUNT         4118 a         6979 c         7034 g         4061 t
ORIGIN      
        1 aggccgcgca cgaccggggc ggtgacgcgg gtggcggtgt cgctgggacc gaggtcgacg
       61 atccacttgg cgcccgcgtc gtagagccgc tcgacctcgg ctacccagtc gaccggttcg
      121 acgaagaccg actgggcgaa ctggtgcgtc agctcggcgt cgatgccggg tgtacgcgcg
      181 gcccaggtgt cgatgaggtc gacggcgtcg gccagccgcg gggtgtggaa gccgacctcg
      241 acctggatgc cgctgaagac ggggttgaag actgccccgc cccggagctt gttcttgcgc
      301 tcggcttctt ccttctcggt gatcttgctg cagtacaact cgaaacggcc cagctgttca
      361 ggggttccgg tgatgacgac cgagcgtcgg ccgttgcgga tcgacaacac cggcggcagc
      421 accgtgcgca catccttgga gaactcgtca agcagctcgg cgatgcggtc cggatagacg
      481 ttggtcaccg acaccatcgg cgacttgtca ccgcggccga ccatgccgcg gcgccgcgac
      541 accagcgagc cggcggcccc gatcagctgc agcagcgcca acagctcggc gtcgcgggcg
      601 ccgttggccc gcagcgattc gcaggcgatg atgccctgcg aatgcccggc catcgcgacc
      661 ggcggggtgt cgtagaggtc aaggccctgc ctggtcaggg cgcggatggc ggccatctgg
      721 gccagcagga tgcccgggcc agagatcgcc gcagtgatca gttgcttggt cgcgggcacc
      781 gactccccgg cggccagcgc cctgacccac tccatcggtt cgaaaccgat cggccgcact
      841 accaccagct cgcgggccac cggctcgagg agcaccgccg cctcaccgac gatctcgttc
      901 agctcgcact cgataccggc gcaggccacc agttcttcca ggttttccag ccaggcgctg
      961 ccctgcccgc cgaaagcgac cgcgtacggc tcgccggcgt tcaaacgatc gaccagggcg
     1021 agggcgtcgt gcgacggcac cggggggact agtgcttcac actcgctggc tgcggacacc
     1081 atgtcgtatt cgtagatcgt cacgccttga atctcctgat tgttttccgt atcgctgccc
     1141 tcggcactac cgacctttat tcggctgcca aaggatccgt cactgctcgt caagaccccg
     1201 tcgaaacaca cccgtcgaca acagcagcca cgctctcttt gcgcccacgt ccaccgccct
     1261 agcgggcttt caacgtcacc ttgggcggcg tcctcgtagc gggtgttcgc cgtccccgct
     1321 gcccgggggt tgcgtcgtgg cggtgaagcg ccggccgaca caacgatctt caacgacaat
     1381 gaccgtcgct cccaacctgt cggcaccact gtttgtggtg gtgtacacag ggtggtgtgt
     1441 tgtgttcgaa gtctgggcgc acgactgacc agcaggtccc ggattggccg acctgagtac
     1501 taacctgtga atggccatcc acatgacatc ggccgtgtgc gttaataatt ggcggctcat
     1561 gggtgataca ggcacaaggt gaggggtcgc ccccgcctga gtgtgcgctg gttccaacca
     1621 gtccgctcga gctgaaatag caagcgacag accgtggttg gagcgaagtt cacctagggt
     1681 agccgtgatc ggtgtgcgcg cttgagcgtg ggcacacagc gatcgacggt gacatcagct
     1741 atcgatagca agcgtcctat cacggaagcc gctggtccgg aaccgcatgt tgctcaggcc
     1801 gacgttttgg acgccggcta tgcaggccgc cacgcggccg cggtcgcggt gaggatctca
     1861 gcgatagctc tcggcgttgc tccgctgccc gccttggttt accgtcatga cggtcaattc
     1921 acaagggcgg tcgtcgtgag aattctggcg ttgggtgccg ctggtgagat tggccgaacc
     1981 gctgcccggg tcctggccgg tgatgaccgc atcacacagt tggtcgtcgc cgaccggaat
     2041 gcgagggcgg cgactcgagt cgcgcgggct ctgggggcta aaggagtccc gctggccctg
     2101 gatgtgaccg accgccccgc gctggacgca gcaatgcgtg attgcgactt cgtgatcaac
     2161 acggttggtc ccttcttttt gttcgggccg ccaattctgt cggcggcgat cgacgcggga
     2221 cgtgactaca tcgatatatg tgacgatccc gaacccacgc tggaaatgct cgcattcgac
     2281 gatcgcgcaa aggccgccgg cgtcacagcg ctgctgggca tgggtgccag tccaggcgtt
     2341 gcgaatctgc tggccgtcat cgccgggcgg gaactcgaga ctgtcgacac ggtgatcacc
     2401 gcgtggaata tcaatgccgc acaatcagat ccggccagga cgggagcgac cagcgccgcg
     2461 attgtgcacg gcatgagaca gatcagcggc accatccccg ttacccgtgc ccgcggactc
     2521 gagcaccggc cggctctagc gaggatcaga gtcagctatc cgggaatcgg ggctgtacgc
     2581 ggtcgtagct tcggccatcc cgagggggtt actctgtcac gcgcctttcc cggactgcga
     2641 gaaagcacga acatggtggt tggcgaccgg ttgaccctgg cagtggtgtc cgtacttcgc
     2701 tgggtaatca accagcgaat catgacgatc gatcgcgctg cttacgtcgc cgcccgcctg
     2761 ggacgtctta tggtcgataa gccggcccat atcctgacgt cgggcggcgc accgccgctc
     2821 ttcgcgcttg cgaccggcac ccggcaggac tccacctgta ccgtagcaac ggcgttggcg
     2881 cagctccctg ggctgtccat ggccgccaat accgcagtcc cgttggccgt ggccatcccg
     2941 ctaattgccg ctagccgccg tccgggagta cacacaccag aaacactgct ggatcccgct
     3001 gctttcttca gcgccttcgc ccgtcactgc atcggtgatc cggccccggc cgccatgacc
     3061 attaccacgc ggtcctgggc gtctccggaa gacaacgcgg cagcgctgaa cgcatccctg
     3121 ctcactgcgc ttctgaatgc ccggtgccga acacacatag ccgacgccct tatttgaatc
     3181 cagacgtagg tctccaccgg gcttcatgcc cgcaagaaac gtcacgcgcg gattgatgag
     3241 atatcgggtt agcgctcacg tggaggaatg cgccggcaag cggaggctcg tgccgtttgt
     3301 cgtggagtcg caggaaatct cactgttgtt tgatgcgcgg ttcgtcttcg tgcctatctt
     3361 cggcaaccac cagttccacc gcccgaacgt tcgcatcaat gcgggcacga gtatgagccg
     3421 tacgatcagc gcatcgatcg caaccgccaa ggcgagcccg aatccaattt gtttgtgctc
     3481 taacacatgt gcagtgacaa aactcgcgaa caccgccacc atgatcgcag ctgccgtcgt
     3541 gatcggccgc gccgtatgag cgagcccgga tgccacggcg tcctcgttgc tctcgccacg
     3601 gtcccaatac tccctgatcc gcccgatcag gaatacttcg taatccatgg aaaggccgaa
     3661 cagtaccgca aaaatgacgg taggcagata cacctgtatg aagcccggac tacgaaaatc
     3721 gaagagggac tcaccggttc cccattggaa gacggctacg gtcaccccca tcgtggcggc
     3781 aatcgcgagc aaattcatga ggatagcttt gatcggcagc aatatgctgc ggaatgcgat
     3841 cacgaggaac acaaaagaga aggccagaac caatccgaaa accagtggtg acttcttgct
     3901 gatctcctcg gagatatcga cgtactgagc ggtggctccg ccgatatgca cagtaggccc
     3961 accgtcgcct gcgagttttc cagcgtcagc acgaacagat cgcacgagtt cggtggcggc
     4021 atcggaatct ataggcacat tgaggattac tgtggcgacc attcgtccgt tatctacttc
     4081 ttggagcacg taggagaccc gatggtcttc ggacaaccgg ccgacgaagg cgccggcttg
     4141 atgcgcctgg gagtccgtca tgggtccgcg atcagaacct gtcatgatcg cctgcaccgg
     4201 cgctagtagc ccgctggtga acttagcctc gatgagttga tttgcgtggc ctgacggttc
     4261 tccggcaagc gcctgcatcc cggcgtctag accgaacttc acaccggtga gcgggagggc
     4321 ggcgagcacg agaactgcga cggccgcggt tgcgaacatc accgggcgac gcatgaccaa
     4381 cttcgcccac cgctgccaac ctccgacgat atgacctgct tgggaatcag cgggcaggaa
     4441 cctggcgggc aacgaaccga aattgatctt cgacccgagt ccggcgagca acgcgggtag
     4501 caaagtcaac gagacgagca gtgccgcgac caccgctgcg gcgaccccga cggcaatcgc
     4561 gcggaagagc ggcatctgca cgacaatcag tgcgccgagc gagaccatca cgatcactcc
     4621 cgacaccaat atggttcgac ctgcagtgtc ggccgccgcc gcgacagcgg ctaccctacg
     4681 tgccgccggg tcacgaatat cgctgtgggc cagttcttct ctgaaacggc tcacaatgaa
     4741 catcgagtaa tcgatgccaa cacctgtgcc gatcatcgtc gtaacggcca agacgagcgt
     4801 atcgaagctc accaatcccg tcatgccgaa gacgaggcca taagcgacag cgatgccggc
     4861 gagtgcaacc acgataggaa gggcagccga gacgagcgtc ccgagggaaa aaatcaagag
     4921 caggagagca accggtaacc cgatcatttc ggcgcgctgc gtatctgcga cagaaacgcg
     4981 gatctggtcg gtctgcattg gggacggtcc tgttatgccc atccacactg cgtccgatga
     5041 aagacgcgcg agcttatctg cgacttccgc agcggcatcc gaccggctgg actggttccc
     5101 cacgagtccg acaagcgcga tcgccgtacc gccgtcgccg gaaatgagtg gcatcgaaat
     5161 tctgtcgaac ggactcacga ccttggccac gcccgggacc gatcgaactt ccgacaatgc
     5221 ccgcagtatc gtttcttgcg aggctggcgc atcgatgtcg tgttgacgga agtcgaacac
     5281 gacgacattt tgctcggcgc caaccgacgg gaagtctcgg tcgaggattc tggccgcctc
     5341 ggtggattcg gcagtggtca cgttgaagtc tggtgccttg aggctctttt caaccgctgg
     5401 catggcgatg atgcatgcgg acaccaccac tgcccaaacg acaagcacgg tccgccaatg
     5461 ggtagccatc aagactgccc atcggtacag gacgccgggc cgaccttgaa ctccccggtc
     5521 cttggaaacg cgcctcgccg tcgacggcgg ctcgacggtc tcgtcgatgt tggtcggcgt
     5581 cgcttttgcg gttagatcgg gcacgactgt catggctcct ccgtgctgct gtgcaggtca
     5641 gaagcagtct cagggtggtc ggtcacgtcg ccgtcacgac aacatcactt gcgatgggtt
     5701 gcgcgttggt ttcaatgcga gccagttgcg cgagcaagta cctccatccc catggcccga
     5761 ccggaacgcg cccaactgac cagccctgat gcttggttaa cggtcgcggt tctgctgaag
     5821 agtctcctca cccggagacg atgcgagcac ggcatcaacg gcggaggtac tagcaggagc
     5881 cacgggggcg tggacgagga ttgcgctggg ttcggcggga accgacgacg gtaggcggcg
     5941 cgataaagcg gatcgtcgac gtgttctccc gtcgcatcct ggcgggcgat gcaagcacaa
     6001 ccaccgattg gtcacgtcgg tgtttggagc aggcagtatt cacccgtcgt taccccgagt
     6061 tccatttcac cgcaagtggt ttggtgcacc actcggatgc ggggcagtac aggtcgacgg
     6121 cgttcaccga ggcgcttcgc ggatcgccgg tcgatcggca aggtcggcga tgccttggac
     6181 aaggcgttga tggaatccgc ggtggaactg tataaatcgg agttgatcga cagtactcga
     6241 cgttcaccgg tcgggccgag agtctgcggc agttccagga cggctcatgg cggtcagcgg
     6301 cgaagcctca cttgttcgcc aaggagccga tgcagtcgtg ccgaaatgac gaacactgca
     6361 gcacatcccg aggatcagcg tgttaagtcc ggcacgcggg ttgccgcgcg cggcggggct
     6421 ggtgtggcgt tgggatatcg ggcgtgctgt ctgaagtcga cgccagcttg tcaaggcagc
     6481 cgcgtgtttg ggaagccggg atacgcttgg cgtgtggccg tcgaggcgga tagttctggg
     6541 tcaggccaac ccagacagca gactcggtgg tcctatcgcg ggccgccgca atcggctgtc
     6601 agttccccgg cccgcaatgc gcactacgcc gagcgaattg cgcgccagcg tcgggttgtc
     6661 aacatcctca cgtggttagc tgcggccttc agcgcgagct ttgcggtgtt gttgttggtc
     6721 gcccagcgcg gttcgatcta tatggcaatc gtcaacgccg tgatggcgtt ggtctttgcg
     6781 acgattccgg tgcttcaccg gttcggcagc ctcgttgcgc ccctgacctt gatcctgacg
     6841 acattcgtgt cgctgtcgat cgtgtgctgg gatatcggca ctggatccgg tctggatctc
     6901 ttttttgtag tggtggccgc ggctgcggtg ttggttctgg gcatcgaaca catcgccctc
     6961 gcctcgatcc tggtggcgat cggcgcggcg ctggtcatca cgctccagtt caccgtcccc
     7021 cccgacaccg gggtgcagcc gccgtggttt gtcgcctttg gcttcattac gtcggtgatc
     7081 tgtgcctgca tcatgagtgt cgcggccgtc tggtacgccc tgcgcgagat cgagcgcgcc
     7141 gaagaagcca tggagctgga atatcagcgt tcggagtcgc tccttgcgaa catcctgccg
     7201 gccagcatcg cgtcacggct gaaaactcag ccgaccagcg tcatcgccga caggtatgcg
     7261 gacgcctcgg tgctgttcgc cgacattgtc ggctacaccg actgggccag ccagaccgca
     7321 cctgcccagc tggttcagtt cttgaatcgg ctctacaccg atcttgaccg attggtcgac
     7381 gaccatgggc tggagaagat caagacgagc ggtgactgct acatcgtggt cagcggtgtt
     7441 ccagagcgcc gcgaggatca tttgcaggcg ctggcgcgcc tcgcgctgag tatggccgct
     7501 gcggtcgacg ggctgagcga tccgcgcgga cgtcggctgc ggctgcggat cgggcttggc
     7561 gcgggtccgg tcgccgccgg cgtggtgggg attcgcaggt tcttctacga cgtgtggggc
     7621 gatgcggtca atgtggcttc acggatggag tccaccggcg tcgagggcaa gatccaggtg
     7681 ccagaggtag tccatggccg gttgaaagac catttcgtgc tcgaggaacg cggcgaggtc
     7741 gtcgtgaagg gcaagggcgt aatgaggaca tggtttctga ttggggaacg aggtcgccca
     7801 accgaggcgg acgcaaggtc aaacagcgcg gaagccccct gagggcggtc gatggctccg
     7861 accacctgtc accgtgaagg acgtcgcagc cgaaccggcg ctcgccggat ggagctgtcg
     7921 atcccaccgc ttggttcaga gcggcagcgg ctagcgccaa tagcgcttgg ctttcctcat
     7981 cgcgaagaat cccagtgaga cgacgtcata acgcgccggc agcaagtttc ccaggtgaag
     8041 ggcaagtgcg gcgatgaccg cccgagtcag taacggtcgg cagtccaacc actcgtccac
     8101 acattcactc agcagttcgc catctttgca gtagagctcg tatgacagca ctcctgtggc
     8161 gagtagggcc cacgcctgcc tggcattcat atcaacctcc ggtcacgatg gtgtgttggg
     8221 tactgcgggt tggtcccgtc tcgaccgccg tcgcttcgtt ggctgtgtct ttcgaccggt
     8281 gcacgttcat gtcgagacct cctcgcaatc actttgttgg cgtttccgtc actcccgctt
     8341 gagattccgg gacatgtcta tgattcggag acccccacgg ccgggccacc ctgtcaacac
     8401 caccattgct ggtcatgtgc acatggtggt gcttgtacat ggtgacgtat gcccatgacc
     8461 taaagcttct agtctgcggg ctgcgttgga agagcagcgg tgtcatatcc acaagcgtcg
     8521 tggcgagcat atggtcgcgt ggcacgaagt tgcggccgga attggcaacc atgacgccgc
     8581 acacaatcgt aggcgtgacg gttctcagtg acccgtatca gtttgtcgag atcgatggcg
     8641 attgcacgag cggcgcctca ctatgaattc ggttcagcaa atgccctgaa agccacagcg
     8701 ctgcatgtca tttcgccgaa ataggacgag accgccaaca atagaacatc gttgagctgt
     8761 ctgatttggt tgccggtaag gcagtcgcac ccgtcgccgc catcaagtgc tccggcgcgt
     8821 cgatccgcca aaggcgttct cggccttcag tttcgcaaga tctccaggtc attgtgcgtg
     8881 tcttcacccg gcggccagca tcgcggtcat ttcgcgggtc ggctgcactc cgagttcttc
     8941 gtcgagcaat ctttcgtaag ttcggtactg gcggattgca tcggccacgt tccccagtcg
     9001 caggtgcgcc ttgaccatca acctgtgcgg actttcgcgc agtgggtcca tcgccaaccc
     9061 ggccaacacc acccgaagcg catcgcggta gcgacccgac tcgcacagtc gctccccgag
     9121 cgcatcgata gtctgcaatc tgatttgtcg gaaccgttcg cgctccacca tcacccattc
     9181 gtcgctccag tcggggagaa gttctccaca gcggccgcct atttcccgcg ccagggccag
     9241 gtcgtgggcg atgtcgtggc gtgcgtcgtg caatcggttg aatgccgcga cctctgcccg
     9301 gtagtcgacg atcacactgt cggccaaccc gacgtgccag gcggaaacga caagggcatc
     9361 cgccatcgct gagacccgcc acagcgccga ccgaagattc gcacgcgcgc gctcctcgga
     9421 ggtgtccggc cagagggttg cagcaatgcg agaccgtctg atggggcgat cagcaagcgc
     9481 gaccagtgcc accagtcgct ggcagcccaa aggcaggggc acgaccgtgt cgcgcacggt
     9541 caagcaaaag tcgtcgatca gatcgaccct gaattctgga accgtgtgtg tttcgccatc
     9601 catggcgttg ccaactcctt ctgatatcgc cgcgccaacc ctggctggca ctcgctgatt
     9661 ggacgactag tccgatatga gatgccaagg ccaagaagtc ctcgacatgc gcttgctctc
     9721 tggactctgg gcttctgtcc gggtcttgct gaataggagt gttcggaggg cgcggcgaat
     9781 acatgacagt cgggtgtatc tgcgtgtccg ccacatcact ccatggagca tgtaggtctt
     9841 tcggctcaag ccgtataagc cgcgtttcgt ccgacacgcg aaagaactgg ttacatgtgg
     9901 cgcaaacctg tgttgctgtt ccccggccag gggacatgcc gcggcatgta cgcgaccgcg
     9961 ctcgagtgtc gcgtcgagat gttcaccgac gacatggaac tcgaggccga cctcggcgtc
    10021 gactcggtga agaagaccgg gccgcggctc gacccgcgcc atcagaagta gaggagaaat
    10081 cgtgaataga cctggggcgg ccgacgatcg gcggcggacc gaaggctctg cgccacgact
    10141 acacatcgcc ggacccgctg atgccctcgc catgggcatc gaaaccgtgt cagagcggtc
    10201 gtccccgtcc cggccgaact cgctgcggga ggccaccgcc gaactgcagg cgcgcaagga
    10261 actggcgacc agaggcgcag agcaggcgac acagcgacag cgtgcccgcg ggaagatgac
    10321 cgtgcgcgag cgtctcgagg cgttgctcga cgagggttcc ttcacggaaa ccggcatgct
    10381 ggcccgtcac cgtgcaagcg ggttcggttt ggaacgtcac cgccccgaca ccgacggcgt
    10441 cgtcaccggt tgggggacca tcgatgaccg caaggtgttc gtctacgctc acgacttccg
    10501 catcttcggt ggatcgctcg gtgaggtgtt cgccagcaag atccacacct tgatggacct
    10561 cgcggccgcg accggcgcgc caatcatcgg actgaacgac gggggcggcg cccggatcca
    10621 agagggcatc accgcgctgg cgggtttcgg cggcatcttc gcgcgcaacg tcagtctgtc
    10681 gggcgtcgtc ccgcagatct ctgtgatcct cggcccctgc gccggcggtg cggcttactc
    10741 gcccgcgcta accgacttcg tgttcgctgt gaaggacaag accaacttgt ttgttacggg
    10801 cccggatgtc gtggaagccg taaccggcga gaagaccacg cacgaggagc tcggcggagc
    10861 cgttacacac gccacccgta ccggtgtggc cagcttcctg atcgacgacg agtccggctg
    10921 tttcgacgag gtccgctact tgttgtcgtt cttgccgtcc aacaaccacg aggacccgcc
    10981 gtctgtcgtg ccgaccgatc ggccctcgcg tcgctgcgac gagctcgtcg agatcgtgcc
    11041 ggccgagccc aaccgcgctt atgacgttcg tgaagtgatc gaggtgatcg tcgatgacgg
    11101 cgaatacctt gaggtacagc cgttgtgggc gaccaacatc gtgtgcgctt ttgcgcgact
    11161 ggatggtcac gtcgttggga tagtggccaa ccagccgagc gtgctggccg gcgcactgaa
    11221 catcgacgcg gcggagaagg ccgccaggtt cgtccgcacc tgcgacgcat tcaacatccc
    11281 cctggtgacc ctggtggacg ttcccggatt tcttcccggc gtggaccagg agcacgccgg
    11341 catcatccgc cgcggcgcca agctgctcta cgcctactgc gagtcgaccg tgccccgggt
    11401 ccaggtgatc ctgcgcaagg cgtacggcgg cgcctacatc gtcatggact cgaagtcact
    11461 cggggccgac ctgtcgttcg cctggccatc caacgagatc gctgtaatgg gcgcggaggg
    11521 cgcggccagc atcatcttcc gcaaggagct cgccgacgcg cccgacccgg tgcaacaccg
    11581 acgaaagctg gtggccgact acaccgagca gctgatgacg ccctacattg cagccgaacg
    11641 agggatggtc gacgatgtca tcgaccccgg cgagacccgg acaattctca tccgttcgct
    11701 tgacatgctg cgctcgaaac gagcatcctc gccgcgccgc aaacacggca acatacccct
    11761 gtgaaaccgg ccatgttcca gatcaccaag ggtgacccca cccctgaaga gatcgcggcg
    11821 ctggtcgcgg tactggctct cgccagcgcc accacccaac ccggctccat cggctccacg
    11881 gcggcgcgct ggcgaggatc cgcaccaccc gccgaattca atagcgctgc actaggccta
    11941 cgagaccgat gctggcgacc ttggggcgcc aggtggtctc gcagtggccc ggcgcatgcc
    12001 ccggcggagc atcgaatcca ctgggcagga acccgcgacg ccagctaacc ccagacgact
    12061 tggcgcgttg agcatccggc gcgcggtccg ataatgggcg gcacgggttc ttggcgcgag
    12121 tgatgcgaca gactctgcgc caacttatgc gtcgccccgt ggtccgggtt tcgtgccgta
    12181 gccgggcatc agcgggctcc gctgatgctg tacacgttga agaaccgtcg ggcctcacga
    12241 gcgtagtcat cgggcatggt ccatgcggcc gcgtctcgta accgagcgac ttcggccatg
    12301 agccagccga ttcggcgatc ttgcgcggcg atcgtttggc gtgcggtgcc gagtcggcgc
    12361 tcgcgatcgg cgagcttgat ttcggctgcc tggcgacgct gctcagactc gcctattacg
    12421 cggcgcaatt cgtcgatgtg tgcgtcgggt gtaagtggca tcatggcgta gtcacctcct
    12481 gtctgtgtgg cggccgatga tagggcatcg tggtttcatg acgtacccgt gcagacaatg
    12541 ccgcgatagc cgcgaagcag ttgcgccaga atcgaattca gcctgcgtac gcaagctccc
    12601 gcccgtccac ctccgacatg gcgtctcgaa gcttgccgag cagcctgcag cgtgttgggc
    12661 cgatactgcc gatcggcatg gcaaggcttg cgcttatctc tgcgtacgaa ggcgctgggt
    12721 gggcgaagag catcgtgatc agttgacgtt gctgctccga gagtttcatc agggcccgat
    12781 gcaccatttg tcgccggtcg gcgtctaaga gccaacggtc gaagtcgacg acgtcgagcg
    12841 gctcgtcgac gccggcgaag tctgccacgg ggcgctcacg gctgcgcgct cgcgccctgg
    12901 caacacactc tcgccggagc gtcgtgatga gccagcccgt cactgcgttc ccatcgcgca
    12961 gcgagccgat attctctacc agtttcaacc agaccgtagc gaccgcgtcc gcggcctcgg
    13021 cttcgcccag tcggtaggcc gagctgaccc ggcgtagcaa cggccccagc ctgtcggtga
    13081 gctgccgcca cgccagttca tcaccggctg cggcacgccg cacaagttgc gcggtgtctt
    13141 gatcgaccat cgtcagttcc ccctaatggc gggattgcgc caaccgctgc ggccgccgcg
    13201 acgccgcccc accgacactc gaggcggcgc ttgggcgcac cagctcagat gtgttgcaac
    13261 ggatgtcggt tgaagcatcg tcccgctcgt atctcgtgtg ttcgcattca cggccgacaa
    13321 tcggccgatg caacgatctt cggagaactg aaggggcgcg aacagcctgg ggacaccacc
    13381 atcggtggtg gtgtgtactt gccacgggcg cctacgcatt ctgaaattga ggcgggtgat
    13441 cgtcacaccc cgcgcgacga gaagagcatt gaaatccccc ggtcgagttc aagatccgcg
    13501 tcggtcacgc cgtccggaac ttgcactgca acgaggtagt cactgttctc cgcggcttgg
    13561 cagcttactt cggctaccaa gccagctgag gacgccggcg ccgactccgg gacttctcgc
    13621 tgcctgtgtc ggccggatta ctcaacgagc gcgccggtat tgacgtgtgg ttcggccgca
    13681 agatggaacc gcgcattgcg acatcaagca acgctgctga agtcgtcggt agtaacgacg
    13741 gccggcgcgc aatttcgatg agaccgagcg ggtcatccgt gtcctgctcg aattcaaggc
    13801 tcgcgaactc gcacaacaga ggcgtggatg cacggcggct tttgcgtaat gcgtctcgtt
    13861 cgtgcaatcg tcttggagcg gcctgcaacg tcatgtagtg cgctaatgcg cgaaacggcg
    13921 ctgatcagaa aattcagcac cgtcatcacc accccggtgc acccaggcgg ctcggacccg
    13981 tcctccgtag tgcatgcgga agaagaccta accctgttca gctacgtgct cacagggccg
    14041 aggcgacgct cggcgaacgt cgaccacatc cagtccggcc gatgcggatg gcgcagcgcc
    14101 cacttggcgg cccgggccac caggcagtta atgcctgcgg ttttgttggg accgtatctt
    14161 gcggtttttg ttgggaatgt attcgggatc tgaacgtact gtcgatcgat gcctaacagt
    14221 gcgacaagat aagcagctcc ggcggcaaag atcaggccag ccctcgggag cgcctgcgag
    14281 tatgcgatgg ccaaatatga gtagcggtct actcgcgcgg acccggtgcc ggtaaggaga
    14341 tgatttcaac ggtcaaccct gatggcctcg agtagcgatc gtggttgagt aatgatgaac
    14401 cacgggggaa ggacagcatt aacatgagtg gtcgcatttc ggtcctgatc gctgatgatt
    14461 gcctgctggt gcgagacagc gtttgccggg cgttgtcgat cgacgccgat gtgactgtcg
    14521 ttggtgtcgc cgaggattac gattccacgg tcgaacttgt cgaaaagcac aaaccgacga
    14581 tattggtcac cgatgtacgc atgccaccga cattgacgga tgagggcatc cggttggcac
    14641 gttggttaag gagggcgcac cccgacatcg gggtgatcgt gttgtcgcaa tatgcggatc
    14701 cgcgttacgc ggcgggtttg ctggatggcg gtacgtcggg ccgtgggtat ctcctgaagc
    14761 aacgcatttc gcatttcgac gaactcagcg aggcggtgca ccaagtcgcg gccggtggga
    14821 cggtgctcga tccgatggtg gtggaggcgc tgctcgccca gccgcgctcc acctcgaccg
    14881 gccgactgac ctccagagaa cgcgaggtgc tcggcgaatt ggcaaccggc ttaagcaatc
    14941 gcactgtggc gcaacggctg gtgttgtctc aaggggcggt cgaaaagcac atcaattcga
    15001 ttttcgcgaa gctgggattg accatggacg actctgttga ccggcgggtc aaggctgtgc
    15061 tgatgttcct cgacgaacgc gcttctgctt gagcatgccc ttcagctggg ctcttgtaat
    15121 tgaactaaag ggccgcgaag ttgcggtcac gaaggctatt ggatcagccc gtgatatcgg
    15181 cctcaacccg tgctggcgag ccggcagtct ctcgcatctg gccgagaacg cgcactacta
    15241 cctggacggc aagccacaat gccagctgat cgtctcggga caccgcgggc aaactcggcg
    15301 atgcgctgcc ggcgggcgtc ggcacgcgca tcccacatat gccggcccga ttcgccgagc
    15361 atgaccaacg acgctcggcc gtcatcggga tcgctccacc tctccagcag cccttgacgt
    15421 tccatgcgct gtaccaattg cgtcattccc gattggctgg cgccctcggc caaagcaagc
    15481 gccgtcaagc gcatcgggcc ttcgcggtac agccggttta gcaccagcgt cgcgcttgca
    15541 gccacaccct cggagcgaac ttttctcggc gtgatcctca tgttgacgtc catgaacctg
    15601 gccattcggc tactcaggag cgcgaatttg aaatggcgtc tgaacagagt ttcttgaagc
    15661 agcccattgc acctggtgcg gtcgcgtcga cgcaaggcaa agttgggact cgccctcgac
    15721 atgtgggcgt cagctgacac tgcggggcgc gcggaagcag ccgtcgaaat gtccgttctc
    15781 gatgcccacg gtttgcatga aggaatgggc cacaaccgga ccgacaaatc gaaatcctcg
    15841 ctgtcgcagt gcagttgcga gctggcgtga ttctgggcta tccgttctgc catcgctcat
    15901 cgtcgcagtg gacgccgacg gcggtgtcgg tagtgccaaa cgatgtcgtt cagcgagatg
    15961 gaggtcagca gccgggcgtt gtgcacggtt gcttcgatct tggcacggtt acggatgatc
    16021 gtccggtcgc tcatcaggag gtcgacgtcg cgatcagtca tgccggcgac gacggcgggg
    16081 tcgaagtcgg cgaaggcccg tcggagcgcc tcgcgcttgc taaagacgac gcgccaggac
    16141 agtccattct cgaagtaggt cgacaccaat tcctcgaaca acgccgcttc atcgtggacg
    16201 ggagtgcccc actccaggtc gtggtatcgc gtcagatccg ggtcagcctc ggttaaccag
    16261 ccgcaccaca acgccgtcgg ctccggtcag tgtggtcgga tgagtcgcgg ggacctggag
    16321 atggcgaacg cgcgcctgta cccgttctgg ggatcgctga ccgaggccct acggaccggg
    16381 cagccgcaga gcgaggcgaa gaccggaggc gacttctttg cggcggtgta cgccgacccc
    16441 aaacggctgg cccagttcgc cgccgcaatg agcgggctca gcgccagcgc aggcgaggcg
    16501 atcgcggctc acttcccttg gcgcgactac ggcagcgtgg tcgatatcgg ttgcgccgag
    16561 ggcgccgtct cggccgcgat cgcgctcgcc cacgagcacg tcaccggcgg cggcttcgac
    16621 ctgcctccgc tcgagcccct cttcaacgcc tacgtcgcgt ccaccggctg ctggccgagc
    16681 tcaacgtccc cgaacccgat gaccgcgaaa acccaaggga acaccacgat acgacggcaa
    16741 ccacttccga atctgcagtc ggcgcctaaa atgaccgcgt ggccgacgct ggcccgttga
    16801 cagtcacact tccacgccga attgcggtga tcgatcggag tggggctgca gttcgcccct
    16861 tgcgatcagt acgaaaaatc aaggccgaat atcactgcgg cataaccagc atcgctcact
    16921 gcaaggcacg tcgcgcgatc attcaggccg ccgaattccg accacggatc gccgaggctg
    16981 tggtgtgtgt actggcctgt gccaccggag acctcctcaa gtacgagctg aactgagctg
    17041 tggaggcgga ctatgtcgcg tatcagttgc cctgtcctat ccgcgaagat cgccacggcc
    17101 gaagaatcca tgcggtacat ccaccacggc gacaacgtgg ggttcagcgg gttcactggc
    17161 gccggctatc ccaaggcggt ccccgccgcg ctcgcggcag ccatggtcca ggcccacgag
    17221 gacggccggg agttccaggt cgggctctgg accggagcgt cgaccgcacc cgacgccgac
    17281 ggcgtactgg ccgaggccca cgggatatcg tcgcggctgc cgtacaactc cgaccccacg
    17341 ctgcgccgcg cgatcaacgc cggcgatgtt cgctacatcg acgcccacct gagccactcc
    17401 gcgcagcaca tgtggttcgg tttctacggc aagctcgacg tggcggtcgt cgaggtgaca
    17461 gcggcgttac cggacggatt actcgtgccg agctcgtctg tgggcaacaa caagacctgg
    17521 ctcgatcagg ccgaccgggt catcctggag gtcaaccatt ggcagccgcg cgagctggag
    17581 ggctttcacg acatctacta cggcaccgcc ctgccgccgc accgccgccc gataatgctc
    17641 atcgaggcac tgcaacgcat cggcgagccc tacctgcacg tcgaccgcga caaggtcgtc
    17701 gccgtggtcg agacgcacag ccccgaccgc aacgcgccgt tcagcccccc ggaccaagcc
    17761 tcgcaggcga tcgccggaca cctggtggac ttcctgcgcc acgacgtccg cgccggacgg
    17821 atgccgccgg ggctgctgcc gatccagtcc ggggtcggca acgtcgccaa tgccgtcctg
    17881 gccgggctcg gcgacagcga gttcaccgac ctggtcgcgt ataccgaggt gctgcaggat
    17941 ggcatgcttg acctgctcga ctccggaacg ctacgggcgg cgtcggccac gtcgttcggg
    18001 ctgtccagcg acggcgtccg ccggttcatg acaggcatcg acggctacaa gggccgcatc
    18061 ctgctgcgca gtcaggagat ttccaaccat ccggagctgg tgcgccggct cggggtgctg
    18121 gccatgaacg gcatgattga ggccgacatc tacggcaacg tgaactccac ccacgtcatg
    18181 ggcagcgcga tcatgaacgg catcggcggc agcggggact tcgcgcgcaa cgcgttctgc
    18241 agtttcttcc tgtcgccctc gacggccaag ggtggcgcca tctcggcgat cgtcccgatg
    18301 gtcagccacg tcgaccacac cgaacacgac gtagacgtcg ttgtgaccga gcagggcctc
    18361 gccgacctgc gcggcctggc tccccgggac cgggcgccac ggatcattca gcgctgcgcg
    18421 cacccgtcgt tccgggatca gctgcgcgat tacttcgacc gtgccttcgc ggctcggccg
    18481 aacgcgcgcc acacccccca cctgctcgac gaggcactgt cgtggcacca gcggtatctc
    18541 gactcgggaa gcatgctccc cgcgttgcag gatggcgccc cgtcggtcag ggcgacgcta
    18601 taacccgcag ccatctaaac aacctttcag cagtactgag gtatgccgac tgagcaggcg
    18661 ctgcccgcga cgcggtcaca acgcgggtcg acgccgggca taggacggag tcgacgacca
    18721 ccccgatcgc tcaggcaatc gatgtcaatg gatgaacacc cggccggctg gtgagcgagg
    18781 gaatcaccgt acgcaaggat atcggcaggc ctgtctcagc gccgaaacac tgctcgtgca
    18841 aaaggatcgc ctggcgcagc aggtacgcga ttgttgtcgc gcacgtgtca cgcccagtgc
    18901 gcaccaggtg gttcttatat ctgctcaaca ccgcggcgga ctcggtatcg gccaccgcat
    18961 acaccagcgg cgccacgaca tcggcgacac cggcgacacg ttgatgtgcg gcgcagcgcc
    19021 aacaaaatct gaagccgcgc acgcgtgcgg cgcacacccg gcacgtgcgc atcggtacac
    19081 gcacgacgtt ggttagggct gcaacggctt caggttgccg cgcatgcgta tgcagcagtg
    19141 aagccactcc cacggcctcc taattgatgt ctcttgcggt gcggagaacg ctaggtcagc
    19201 gttccgacgc ggcgcgccag ttcacgcgga tgaactaaca gcgaagcgct tgtgcgcacc
    19261 gcccggggga tgtgcggcga tgcgcactcc ccccgaggat gtgcgccgcg cccgatcagc
    19321 gtcggcgacg cacaggatgc gcgctgatga atcgacgcaa atgtgctgtg cagagaaagg
    19381 gggaaggtgg aattcggatg ggttggggga attaggttaa gcacaagggg ggcagtcctt
    19441 ttgagaagta tcgggaagat ggagatgtgg gctatggaga attgtggatg gaagtgtgat
    19501 ggtctggcgt gatcgaggag agaggcaagt ttggcgaccc gcccgcggcc ctttgtgccg
    19561 cggcttcgcc gtgcgacgcg gcaaggcgtt tcgccaaaat tctgcacaaa gttgtggctg
    19621 tagagaaata cagtgatgtt ggatgccctg atgacaacac cgaggcaacc aaacgcctgc
    19681 cgccgcacga agccgactgg gcgacaacgt cacaagcgcg tccagcgcgg ccgacaggct
    19741 cttatcccgc atagctcttc ggagggtcgt gaatcaaatt ggaagacaaa gcgttcaatg
    19801 agctacctct gctgctcgcg gtgcccaggg ccgcgcagct gctgggaatc agccgggctt
    19861 ccgcataccg acttgcggcc agcggagagc tgccggttcg ccggctcggc ggccgcgtct
    19921 acgtagtgac cgccggcctt cgtgagctgt cctcgtgaaa ggtaccggcg ggatccaagt
    19981 gctactgcaa ataccccgtt cccagcctga cccatcggct ggaaggtttc cgtggatgac
    20041 caagggcgga catgacagcg aacaggatgc ctgtttgagt gattagcgcc gctgtcagat
    20101 ggatctccct tcccgacgat cgacgatccg acagcggcgc tgcgttccga tcgtccgtcg
    20161 aacctttgcc gcgactggat cacgaccacc gaggaaaccg gccaatgagc accgacaccg
    20221 cacccgccga gcagcccagg aagcgcggcg agcgcaccga cagctcaatc aggaagttgc
    20281 ccaacggcaa aggttggcgc gcacggccga cgctcggcac cgaccccgtg acgggcaagc
    20341 aggtccggcc gagcaaggtg ttcgcgacca aaaaggctgc ccagggttgg gttaccgagc
    20401 agcgccaaca gtggagcacc gcgacgtggg taccgaagtc aagtcggacg ttcgacgagg
    20461 tcgccgacca ctggctgacg cttcgcgagg ccgacatgtc gatcgggccg aataccgttc
    20521 gggctgaccg cgagtcgctg gcttacgcgc ggcgggcctt cggcgcggtg ccagtgcaga
    20581 agctcacccc cgccgcactc gctgactggt cggccacgct gaccggcaag gacagtcgac
    20641 ctctggcgcc ggcgactaag cgccgcgcga tcgtccggct taaatcggtc atggcgcacg
    20701 cacgcaagat gcggtggctc acctatgacc cgtcagctga ccttgcgtca cccgaacaaa
    20761 aggcggtcac cgccaccgca gccgatgaca tttggacacc cgaccagatg gggaaggttc
    20821 tcgatcacgt ctcggcgcat cgcctcggcg ggtgtttcgc gctgacgctg ctgggcctgc
    20881 gccgcgagga agtcggcggg ctgcgctggt cggacatcga ccttgacgca ggcacgctgc
    20941 gaatccgcca ggcccgagtc gacgtgaacg ggcgcgatac cattgtgacg accaagaccc
    21001 agcgcagcgc ccgcgacctg ccgctcccac cacacgagct ggcgatgatc aaggccatgc
    21061 ggacgttcca cttgcgcgag cgcctagccg tgggccggcc actggccgcc gatgacctct
    21121 tgctctcgcg gacggacggg acacggttgc cggtgcgcga ctactcgcgg gagttcgccg
    21181 cgcagcgcag ggcggcggga cttaaggcga tcccgctggg caagctgcgg cactcgaata
    21241 tctctcggat gcgcgcagcg ggcatcgccg ccgacgtggt cgccgcgtgg cacggcgaca
    21301 ccgaacgaat gactcaggcc gtctacgggc gggtgaccga cgaccggctg accgccacgt
    21361 cggcgggttt tctcggccgg ggcgatagga cagagttagg acaagtgagt aggtttcact
    21421 ccccaatagc aaagaatccc aggcaaatag cctgggattt cctttcgtgg agctaagggg
    21481 actcgaaccc ctacgaattc ctcccgaaat accgtctgag ctgcggcgat tgtttttccg
    21541 gtgagttacg cgatgccgtc atgtactggc gatatgcctc ggtgtgttac gcgacgtaac
    21601 agtgctggcg gccggtcggc gggatacacc tcacaggttc cacacccgcc gccgcccgtg
    21661 gccaacctac aagatggcgg tcgattccac ccgcgtgtac gagtgtgatt agttggccaa
    21721 ggcgcgctgc ctgtgcaaga ggctggcgtt gaaataggtc aggtaggcaa gtgtggttgc
    21781 tagggcgggt cgacgagttc gagagttgag cagcgctaga ccgagtgcag cttcgatgct
    21841 gccgttgaca agaacgtggg cacggatgtt gtcctttaag ccaaccgatt taccggttcg
    21901 aacgcagatg ggcacactaa gtgcgcaaca ccggacaccg tgagagcaag tccgccgaac
    21961 aatgcccatc gttcagatgg cttgcgcttc gacttcatgg cgtcgacccc tcgtggagga
    22021 ggacgggtag ttgtataggc ccgcgaagtg agctattggt ggaccacgtt gtggggccgg
    22081 tggtttggat tcgtgaaacg cggtcgacca gttcacggag tacggcttgt gcctccatgc
    22141 gtgccagtgg cgctccgagg catagatgtg gaccgtagcc aaatccgacg tg
//
LOCUS       DASRJA010000591         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2319761,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000591 DASRJA010000000
VERSION     DASRJA010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2319761"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFC01_26300"
     CDS             <1..245
                     /locus_tag="VFC01_26300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83161.1"
                     /translation="EPVIEEVSQPPVLITAQEVAFSTAAAVSLPRAKPTHGVIAALRA
                     LFLSPPEDARPVPRHYPPRRDEFLEEAAMRREMYRL"
     gene            272..973
                     /locus_tag="VFC01_26305"
     CDS             272..973
                     /locus_tag="VFC01_26305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN83162.1"
                     /translation="MTPLLQLEGVVKRYRRGDEQLNVLVDFDFTLDAGEFVVVTGPSG
                     AGKSTLLHIAGGLDAPDNGVVAVAGQDVWAMSANARAAFRRRNLGFVFQFFNLVPMLT
                     AVENVSLPLVLDGTPARSADARAEELLQRVGLGDRARHRPAELSGGQMQRVAVARALV
                     ARPSIILADEPTGNLDSHSSAEVLDLLRSLADEDGAAVVMVTHDQAAARYGSREVHLV
                     DGRVCATDTDSVGER"
     gene            973..3531
                     /locus_tag="VFC01_26310"
     CDS             973..3531
                     /locus_tag="VFC01_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN83163.1"
                     /translation="MQPLRAGWLSFRRIHLVALIADWRRTLLSVIGVAVGVTIVLGTL
                     ILKSELTRPFESFGPSLAHAADAGVVEVTPNISGRLPIETVDRLRTEVAEAEAVIPVV
                     ASLTPVNVAGGTHGFFLLGGTCKVELLVGPFNCEQRARAGKPADGPGVPLQIPAVVAQ
                     RHGLLLGDELRIPGLPPGSAHLGWTFSEFDRVEGINDGYVLLAPSVHIAARLVGSPGY
                     ATAAFVLPRPGTDIKADIDRVIAGVATAGPPRPHLPAMLENGSQSFNLTALVGVLVGF
                     LIAVNTILLAVEDRRAVMGTIGAIGAKPVGLFGGMLGEGAVVGVLGGLLGVPSGFLLG
                     TYLVDRFGQSMLAGSGGTIAAHFTPGLIVIGAAAGTVSGVLAMIGPAVRLVREGPLSS
                     MACVGGVQRVRRIPLWPLVAGAAMLVCAVVILKIFERGSLPLDVGINGMSVGQCGVAL
                     VTLWIAPRGAVLLIELLTVTRRDVGRLLRADVQRYALLFGMSAAVLAAGTSLAVGSQS
                     MQLLGTQQVASQKADRLPDALLIAAQSVLDQRDGHLADSTFKLVEGAADGRGVSSRWR
                     STISSGTLSRLVIGVTPGDWYSEALYQPTGQPDRLWQGLGEGDIGLSEIAAGRLGVTP
                     GETVELPTVGGSKRYRVAGIFRPRMINDAAVGDIVLVSERLARVDWAAVRDQVAVRYS
                     SPADATAHRDDFLDLGAGLSVYDNQQWRAVATNGITRFLEPFTIAGYIVMAAAGLSVL
                     NVFVLGLVQRKRERAALRAIGATPRQEQVVVIAHAGLLGLLVAVLGALGGVGLTYLWS
                     LGSPVYYGIKIDWGVPELALRTGVAAVIVLVLAAALYPVIHARRLETAEILQTS"
BASE COUNT          594 a         1158 c         1294 g          736 t
ORIGIN      
        1 ctgaacctgt catcgaagag gtgagtcagc cgccggttct catcaccgcg caggaagtcg
       61 ctttcagcac ggcggctgct gtgtcgttgc cgcgcgcgaa gcccacccac ggagtgatcg
      121 ctgcattgcg cgctctgttc ctgagtccac ctgaggacgc acggccagta ccgcgtcact
      181 atccaccgcg gcgagacgaa ttcctcgagg aggccgccat gcggcgagag atgtacaggc
      241 tgtgaccgcc tttgcagatc gcggcgatcc tatgacgccg ctactgcagc tcgagggcgt
      301 cgtcaagcgg taccgtcgag gcgacgagca attgaatgtg ctcgtcgact tcgacttcac
      361 tttggacgca ggcgaatttg tcgtcgtgac cggaccgtcg ggagccggga agtcgaccct
      421 actgcatatc gcgggtgggc tggatgcgcc ggacaacggc gttgtggccg tcgccggtca
      481 ggacgtgtgg gcgatgagcg ccaacgcgcg ggcagcgttc cggcggcgca acctcggctt
      541 cgtattccag ttcttcaatt tggtgcccat gctgaccgcg gtcgagaacg tgtcgttgcc
      601 gctggtgctc gacggtacgc cggcgcggtc ggcggacgca cgcgccgagg agctcctgca
      661 acgggtcggc ctcggcgacc gggcccggca ccggccggcc gaactatcgg gcggacagat
      721 gcaacgggtg gccgtggcgc gcgcgctggt agcccggccg tccatcattc tcgccgacga
      781 gccgaccgga aatctggaca gccattcatc ggctgaggtt ttggatctcc tgcgctcgct
      841 ggctgacgaa gacggtgcgg cggtcgtcat ggtgacgcac gatcaggccg cggcgcgcta
      901 cggctcccgc gaagtgcacc ttgtggacgg gcgtgtgtgc gcaacggaca cggactccgt
      961 cggggagagg tgatgcaacc gctgcgcgcc ggatggttgt ctttccggcg gatccacctt
     1021 gttgcattga tcgccgactg gcgtcgcact ctgctcagcg tgatcggcgt cgcggtgggt
     1081 gtgacgattg tacttgggac gttgatcctc aaatccgagc tgacccgccc gttcgaatca
     1141 ttcggtccgt cccttgccca cgccgccgac gctggcgtag tcgaggtaac gccgaacata
     1201 agcggccgct tgccaattga gacagtcgat cgcctgcgca ccgaggtggc agaggcggag
     1261 gcggtcatcc cggtcgtcgc ctccctgacg ccagtgaacg tcgccggcgg cacccacggt
     1321 ttctttctgc tcggcgggac gtgcaaggtc gagctgctgg tcgggccgtt caattgcgag
     1381 cagcgcgccc gcgccgggaa gcctgcagac ggccccggcg tgccactgca gattccggcg
     1441 gtcgtcgccc agcggcacgg cctactgctc ggcgacgaat tgcgcatccc cggcctgcct
     1501 cccggttcgg cacacctggg ttggacgttt tcggagttcg accgcgtcga gggcatcaac
     1561 gacggctacg tactgctggc cccctcggtg cacatcgccg cgcgcctggt cggctcgccc
     1621 ggttacgcaa cggccgcgtt cgtattgcca agaccgggca ctgacatcaa agccgacatc
     1681 gaccgcgtca tcgccggcgt cgcaacggcg ggtccacccc ggccgcatct gcctgccatg
     1741 ctcgagaacg gaagccagag cttcaacctg actgcgctgg tcggcgtcct ggtcggcttc
     1801 ctcatcgcgg tcaacacgat cctgctcgcc gtcgaggacc gacgggcggt aatgggaact
     1861 atcggcgcga tcggcgccaa gcctgtcggg ttgttcggcg gaatgctcgg tgaaggcgcc
     1921 gtagtcggtg ttctcggggg gctgttggga gtgcccagtg gcttcctgct cgggacgtat
     1981 ctagtggacc ggttcgggca gtccatgttg gccggctccg gcggcaccat cgctgcgcac
     2041 ttcaccccgg gcttgatcgt gatcggagca gccgcgggca ccgtctcggg agtgctcgcg
     2101 atgatcggcc cggccgtcag actggtccgc gaagggccgc tgtcgtcgat ggcgtgcgtc
     2161 ggcggagtgc agcgcgtgag gcggatcccg ttgtggccgc tcgtcgccgg ggcggcgatg
     2221 ctggtgtgcg ccgtcgtcat attgaagatt tttgagcgcg ggtcgctgcc gctcgacgtg
     2281 ggcatcaacg gaatgtcggt ggggcaatgc ggagtggcgt tagtgacact gtggatcgcc
     2341 ccgcgtggtg ccgttctgtt gatcgagttg ctgacagtca cgcggcgcga cgtcgggcgt
     2401 ctactgcgtg ccgatgttca gcgctatgca ctgctgttcg gtatgtccgc cgcggtactc
     2461 gcagccggca ccagcttggc tgtcggctca cagagcatgc agctgctcgg cacccagcag
     2521 gttgcatcgc agaaggctga ccgcctcccc gacgccctgt tgattgccgc ccaatcggta
     2581 cttgatcagc gggatggcca cctcgccgac tccacgttca agttggtcga aggtgcggcg
     2641 gacgggcggg gcgtgtcttc gcggtggcga tcgacgatct cgtcagggac gttgtcgcgt
     2701 ctcgtgattg gcgttacgcc gggtgattgg tacagcgaag cgctgtatca gccgaccggt
     2761 cagccggaca ggttgtggca ggggttagga gaaggagata tcggcctgag cgagatcgcc
     2821 gccggccggc tcggagttac tccgggcgaa acggtcgagc tgcccaccgt cggagggtcg
     2881 aagcgatatc gggtggccgg tatcttccgt ccgcgaatga tcaatgacgc tgcggtgggt
     2941 gacatcgtcc tcgtgtcaga gaggttggcc cgcgtcgatt gggcagctgt acgagaccag
     3001 gtcgcggtgc gctattcgtc ccccgcggat gcaaccgccc accgtgacga cttcctcgac
     3061 ctcggagctg gactgtcggt gtacgacaac caacagtggc gcgcggtcgc cacgaacgga
     3121 atcacccgat tcttggagcc gttcacgatc gccggataca tcgtgatggc ggccgccggc
     3181 ttgagcgtgc tgaatgtgtt cgtgctggga cttgttcaac gcaagcggga acgggccgcg
     3241 ttgcgggcga tcggtgcgac accccggcaa gagcaggtcg tcgtcatcgc tcacgccgga
     3301 ctacttggtc tactggtcgc tgttctcggt gcgcttgggg gcgtgggcct tacgtatttg
     3361 tggtcgctgg gctcacccgt atattacggc atcaaaatcg actggggtgt acccgaactc
     3421 gcactgcgaa ccggtgttgc cgcagtgatc gtgttggtcc tggcggcggc gctgtatcca
     3481 gtgatccatg cgcgacggtt ggaaacagcc gagatactgc aaaccagttg acgtcggtcc
     3541 tatttcgatg cgtgcgcttg ctgaccgcgt gcctcacagg cggattgtca cctcagtagg
     3601 accgcgccga aaccggacca tgttgaagta gtgacctact gcgcttgtca cgccgattgt
     3661 cagcaacgat ttcaattgcg aagcgagaca ccgagtccgg tggctgtcgt ttcgccagaa
     3721 catcgcggaa ggaaagacaa tgggttatgc aagcacatct gttgtatcgg ggtcatgccg
     3781 gc
//
LOCUS       DASRJA010000592         8512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1485759,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000592 DASRJA010000000
VERSION     DASRJA010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8512
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485759"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFC01_26315"
     CDS             <1..183
                     /locus_tag="VFC01_26315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZN83164.1"
                     /translation="AFRDYFKSTYGPTIAVYRNIEGDADRVAELDAAIAEVGDSVLRG
                     SSTMEWEYLLLTARKA"
     gene            196..1257
                     /locus_tag="VFC01_26320"
     CDS             196..1257
                     /locus_tag="VFC01_26320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZN83165.1"
                     /translation="MPDDAQQLPTLSDDDQAALQRWVRAQGLGSDVTNVEPLTGGTQN
                     IVVRVRVDGRPMVLRRPPLHPRPTSDKTMLREIAALRTLAGTPVPHPGFIAGCDDLDV
                     LGVVFYLMEEVDGFNPGDSMDEAYVRNAEWRHQVGLSYAGSLARLGNVEWEGKPLAEL
                     KRPGSFLERQVPQFLRLLESYRHDRYSPESLAVTELAEWLEAHRPDDGKPGIMHGDPH
                     LSNVLLRRDKPELAAFVDWEMCTVGDPLLDLGWMLICWPLETSTITAGAQLAALGGLA
                     SRRELLDAYLAAGGRETASLDWYVAMACFKLGIVIEGTWSRYLVGQASRDAGERLHTN
                     AQNLVDIGTRVAKGDSPFE"
     gene            complement(1306..3093)
                     /locus_tag="VFC01_26325"
     CDS             complement(1306..3093)
                     /locus_tag="VFC01_26325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZN83166.1"
                     /translation="MKTSIATVSLSGSLTEKLHACAAAGFDGVELFEPDLIANDHSPE
                     EIRALADRLGLSLELYQPLRDIEGVDERVFADNLRRAGATFATAQRLGIGVVLVCSNV
                     ATATVDSDEVSADQLRRLGDLARQYDIKVAFEALAWGRFVDDYRRAWHIVQLADHPNV
                     GICLDSFHVLSRGHDPADIENIPAEKIFFLQLADAPALKMDVLSWSRHHRLFPGEGDF
                     DLATFVSHALATGYDGPLSLEVFNDTFRQTDPDRTAVHALRSLIWLQDRTAGQNPEGW
                     RNDLTALTAAKPPVGFDFVEIKAEDTSEVEVLLQQLGFTPSGRHRTKAVSLWLAGDAR
                     VVLNEQQARDQIPHVAAVGVLVPDSAATSRRAAELMAPVAYRRTYASEQILGAAIAPD
                     GTEMFWVTEPAWVEEFENGLPLAPTPVRTIDHVNLSQPWQTLDDAVLFFTSVFGLSAD
                     VPTEVPGPKGLIRSQSMRTTDGAVRLLLNVAPHVLDGAGMPQHVAFACSDVIGLARAA
                     RDRGAEFLPVSDNYYDYLAGRFGLDDAAVAELRDLGLLYDRDAHGNEFVHFYTRTIGT
                     VFFEFVQRRGDYDGYGIDNAPVRLAAQRA"
     gene            3259..3900
                     /locus_tag="VFC01_26330"
     CDS             3259..3900
                     /locus_tag="VFC01_26330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010841266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN83167.1"
                     /translation="MTAEPRPEPLRDAERTRSELIEVATAVFAEQGYSGARVDEIAER
                     TRTTKRMIYYYFGGKEQLYLAVLENAYRGIRQAEQRVQVDHVDPVQAIRAVAEMTFDH
                     HIEHQDFIRLVSIENIHRGEFIGRLDSLRALGQPATSLLDQILAQGREQGVFRDDVDA
                     LDVHIVISSYCVFQVANRYTFGYLFDIDFTEQQTHAHLRRMLGDVVVGWLTSR"
     gene            4140..4991
                     /locus_tag="VFC01_26335"
     CDS             4140..4991
                     /locus_tag="VFC01_26335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZN83168.1"
                     /translation="MAPYLVGLVGTGVGPSLTPALHMAEARAQGLDYVYRTIDLAEAG
                     IAPERIGEVLDWARALGFNALNITHPCKRLVLEHLDDIDDVAAELGAVNTVVFDGPQT
                     VGYNTDTTGFALGFREGLPDAATDNVVLVGAGGAGAAVGDALLRLGTDHLTIVDLDIE
                     RATALAREFAARRPDSRVDASSPDKLPVLLPGSDGVVHCTPTGMAEHPGMPFATELLH
                     PGLWVADIVYRPLDTELLTAALQVGCRVCDGGHMAVHQATEAFELITGITPDTERMSR
                     HFRRLVG"
     gene            5023..6378
                     /locus_tag="VFC01_26340"
     CDS             5023..6378
                     /locus_tag="VFC01_26340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010841268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN83169.1"
                     /translation="MSGIETTKPDGVGDHSPRRTPGRAALASFMGSAVEYYDFFVFGT
                     AAALIFPKVFFPSGDEAALVMSFATFGVAYVARPVGAFVLGHFGDRIGRRNVLMFTLV
                     LMGVSTFLIGCLPTFATAGWIAPGLLVVCRLMQGFSAAGEQAGASSLTLEHAPDHRRA
                     FFTSWTLTGTQGGLILAQLVMLPFVSLPDDAELSWGWRVPFWLSAVVVVVAYFIRRRL
                     HETPEFDEAKATGAIARMPLKPLLQHHWKDVLRVIFCAFIAAVSTVFGTLAIAYGKKV
                     GLEAGITLWLVVVANIVALFTQPLFGILADRIGRKPVFIYGALSSAALMPFYMLSMSG
                     DNTVLTFALAVATFSFGYAAANAVWPSFYGEMFSTQVRFSGMAIGTQLGFLLAGFAPS
                     IVTALGGVREGGWVVISAFTAVVCVIAATSASTARETKNVPTPLLGRDLPDSQKVLVN
                     R"
     gene            complement(6399..7148)
                     /locus_tag="VFC01_26345"
     CDS             complement(6399..7148)
                     /locus_tag="VFC01_26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006369656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN83170.1"
                     /translation="MAGNTSEPGATVTSRLLSVLAAFDEGHRSLTLTELARRAVIPVP
                     TAHRLVGELVAGGALHRLDDGRYVIGRLLWEAGLLAPVQGRLRQVAEPFLHDVYAATL
                     ATVHLAVRDGDEVLYLHRMRGRASVPIVSTVGTRLPMHATGVGKVLLAHAPSDVHHRV
                     FAKLTRITPYTITHPEVLDAQLDRIRRDGVATTSEEMSLGACSLAVPVVRASDDSVAA
                     AIGVVVATLKRDRQRLLGALQVAARGIGRLL"
     gene            7219..7998
                     /gene="pcaH"
                     /locus_tag="VFC01_26350"
     CDS             7219..7998
                     /gene="pcaH"
                     /locus_tag="VFC01_26350"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014360281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HZN83171.1"
                     /translation="MTAIVDTNPDSASASQAGISAEIAAVEADYHRAGIEETQPRLNY
                     PPYRSSLLRHPTKELHHADPEAVELWAPCFGAGDVAAVESDLTIQHGGEPIGERMVVT
                     GRVIDGDGRPVRRQLVEIWQANASGRYAHKRDQHPAPLDPNFTGVGRCLTDDDGTYRF
                     TTIKPGPYPWKNHHNAWRPAHIHFSLFGTDFTQRMITQMYFPGDPLFALDPIYQSITD
                     QKARDRLVAAYDHDVTTHEWATGYRWDIVLTGSARTPMEAE"
     gene            7995..>8512
                     /gene="pcaG"
                     /locus_tag="VFC01_26355"
     CDS             7995..>8512
                     /gene="pcaG"
                     /locus_tag="VFC01_26355"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014927450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HZN83172.1"
                     /translation="MSTLTATPGQTIGPFFGYALPFSRGNELVQPGSAGAIRLHGVVT
                     DGEGAPVPDALLEIWQADADGVVPSATGSLRRDGWTFTGWGRASTDDDGHYSFTTVKP
                     GAVQPDSAPFIAVTVFARGLLNRLFTRAYVPRDRLAQDRLLSTVPADRRHTLIAVPGE
                     HGLRFDVHLQGQ"
BASE COUNT         1439 a         2849 c         2763 g         1461 t
ORIGIN      
        1 gccttccgtg actacttcaa gtccacctat ggtccgacga tcgcggtgta ccgcaacatc
       61 gagggcgacg cggaccgcgt ggccgagctc gatgcggcga tcgcagaggt gggcgacagc
      121 gttctgcgcg gctcgtcgac catggaatgg gagtacttgc tgctgacggc acgcaaggcc
      181 tgagaacatg tcggcatgcc cgacgacgcg cagcagctgc cgaccctgag cgacgacgac
      241 caggctgcgt tgcagcgatg ggttcgcgca cagggcctcg ggtctgacgt caccaatgtc
      301 gagccgctga ccggcggcac ccagaacatc gtggtgcggg tgcgcgtcga cgggcgtccg
      361 atggtgctgc ggcggccgcc ccttcaccct cggccgacga gcgacaagac gatgctgcgg
      421 gagatcgcgg cattgcgaac gctcgcagga acaccggtgc cgcacccggg ctttatcgcc
      481 ggctgcgacg acctcgacgt gctcggcgtg gtgttctatc tgatggagga ggtcgacggc
      541 ttcaaccccg gcgacagcat ggacgaggcg tacgtccgta acgccgagtg gcggcatcag
      601 gtgggcctgt cctatgcggg cagcctggcg cgattgggca atgtggaatg ggagggcaag
      661 ccccttgcgg agctgaaacg gccgggttcc tttctggagc gccaggttcc gcagttcctt
      721 cggctgctgg aaagctatcg gcacgaccgc tattcgcccg aatcgctggc cgtcaccgag
      781 ttggcggagt ggctggaggc ccaccggcct gacgacggaa agccgggaat catgcacggc
      841 gacccgcatc tgagcaacgt gctgctgcgc cgcgacaaac cggaattggc cgcgttcgtc
      901 gactgggaga tgtgtaccgt cggcgacccg ctgctggatt tgggttggat gctgatctgc
      961 tggccgctgg agaccagcac gatcaccgcc ggggcgcaac tggccgcgct gggcgggctg
     1021 gccagccgcc gcgagttgct cgacgcctac ctggccgcgg ggggacgcga aacggcaagc
     1081 ctcgattggt atgtcgcgat ggcgtgtttc aagctgggca tcgtgatcga gggcacctgg
     1141 tcgcggtact tggtcggaca agcaagcagg gacgcggggg agcggttgca tacgaacgcg
     1201 cagaacctgg tcgacatcgg cacccgagtg gctaagggcg acagcccgtt cgagtgaggt
     1261 ccaggttggg accgttgacg cggagcaatc gatgtcgatt ggcggtcatg cgcgctgggc
     1321 cgccagacgg actggtgcat tgtcgatgcc gtatccgtcg taatcaccgc ggcgctggac
     1381 gaattcgaag aacacagtgc cgatcgtgcg ggtgtagaag tgcacgaact cgtttccgtg
     1441 tgcgtcgcgg tcgtagagca gcccgaggtc tcgcagctcg gccacggcgg cgtcgtcgag
     1501 gccgaaccgg ccggccagat agtcgtagta gttatccgat acgggcagga attccgcacc
     1561 gcggtcgcga gcggcgcgcg ccagtccgat cacgtccgaa cacgcgaacg cgacatgttg
     1621 tggcatgccc gcgccatcga gaacgtgcgg agcgacgttg agcaacagcc ggacggcacc
     1681 gtctgtcgtc cgcatagatt ggctgcggat caaccccttt ggtcccggca cttccgtcgg
     1741 cacatccgcg gacaacccga acacgctggt gaagaatagc accgcatcgt cgagtgtctg
     1801 ccagggctgc gacaggttga cgtggtcgat ggtgcgtacc ggcgtcggcg ccaacggcag
     1861 tccgttctcg aattcctcga cccacgccgg ctccgtcacc cagaacatct cggtaccgtc
     1921 gggagcgatt gcggcgccga ggatttgttc agacgcgtag gtgcggcggt aggcgacagg
     1981 ggccatcagt tcggcggcgc gccgcgaagt cgctgccgag tcggggacga gcacgccgac
     2041 agcggccacg tgtgggatct gatctcgggc ctgctgttcg ttgagcacga cacgggcgtc
     2101 gccggccaac cacagcgaga cggccttcgt gcgatggcgg ccgctcgggg tgaaaccgag
     2161 ttgctgcaac agcacctcga cctcgctggt gtcctcggcc ttgatttcca cgaagtcgaa
     2221 gccgacgggc ggcttggcag cggtgagcgc ggtgaggtcg ttccgccaac cttccggatt
     2281 ctggccggcg gtcctgtcct gcaaccagat cagcgagcgc agcgcgtgca cggcggtgcg
     2341 atcgggatcg gtctggcgga acgtgtcgtt gaagacctcc agcgacagcg ggccgtcgta
     2401 tccggtggca agcgcatgcg agacaaacgt ggccaagtcg aagtccccct cgccagggaa
     2461 gaggcggtgg tggcggctcc acgacagcac gtccatcttc agcgccggcg catcggcaag
     2521 ctggaggaag aagatcttct ccgccggaat gttctcgatg tcggccggat cgtgcccccg
     2581 agaaagcaca tggaagctgt ccagacatat cccgacgttc gggtggtcgg cgagctgcac
     2641 gatgtgccat gcgcggcggt agtcgtcgac gaagcggccc caggccagcg cctcgaacgc
     2701 caccttgatg tcgtactgcc gcgccaagtc accgagccgg cgcaactggt cggcggagac
     2761 ctcgtcggag tcgacggtgg cggtggcgac gttgctgcaa accagcacca caccgatgcc
     2821 gaggcgctgt gccgtggcga aagtggcacc cgcgcggcgc aggttgtcgg cgaagacccg
     2881 ttcgtcgacg ccctcgatgt cgcgcagggg ttggtagagc tccagcgaca gcccaagtct
     2941 gtcggcgagg gctcgtatct cttcggggct gtggtcgttg gcgatcaggt cgggttcgaa
     3001 gagctccaca ccgtcaaagc cggccgccgc gcaggcgtgc agcttctcgg tgagcgagcc
     3061 gctcagtgac accgtggcga tggatgtctt catggcatgt cctccatgac ctaatgtacg
     3121 aaatagttag ttacaccaca aggcgtggct cgtgtgacgt ccgaacgggc agatcgagga
     3181 acaggcggga gaagcgagat gcaaaacgtc gatctcggcg cagctaggtc tgggcccgcc
     3241 gccagtaagc tccccggtgt gaccgccgag cctcgtccgg agccgctgcg cgacgccgag
     3301 cgcacccggt ccgagctgat cgaggtcgcc accgcggtgt ttgccgagca ggggtattcc
     3361 ggcgcccgcg tcgacgagat cgccgagcgc acccgcacca ccaagcgaat gatctattac
     3421 tacttcggcg gcaaggaaca gctgtatcta gcggtcctgg agaacgcgta ccgggggatc
     3481 cgacaggccg agcagcgggt gcaggtcgac cacgtcgacc cggttcaggc catccgggcg
     3541 gtggcggaaa tgaccttcga ccaccacatc gagcatcaag acttcatccg gctggtctcc
     3601 atcgagaaca tccaccgcgg cgagttcatc ggccgtctcg attcgttgcg cgcgctcggc
     3661 cagccagcca cgtcactgct ggatcagatc ctcgcccagg gccgtgagca gggcgtattc
     3721 cgcgacgatg tcgacgccct cgatgtacac atcgtgatca gctcgtactg cgtcttccag
     3781 gtcgccaacc gctacacctt cggctacctg ttcgacatcg acttcaccga acaacagacc
     3841 cacgctcacc tgcggcgaat gctcggtgat gtggtcgtcg gctggttgac ctcgcgctga
     3901 cccgctgagt cataactcgg ctcccactcg ctccggtcct cggtcgtctg cctcggctcc
     3961 cgctcgctcc gggcctcggt cgtttgcctc ggctcccact cgctccgggc ctcgcttcgc
     4021 tgcgatcctc actcgcgctc gccttcggga tcccgatcac attgtgccct tgacgcgacg
     4081 cggatcactg tgctccactg agcgtacgta ctagttcgta cattacggag acggagtaga
     4141 tggcccctta tctggtcggc ctcgtcggga ccggtgtcgg cccgtcgctc acccccgcgc
     4201 tgcatatggc cgaggcccgc gcgcagggcc tcgactacgt ctaccgcacg atcgatctcg
     4261 ccgaggccgg catcgcaccc gaacgcatcg gcgaagtgct cgactgggcc cgggccctcg
     4321 gcttcaacgc gctgaacatc acccacccgt gcaagcgact ggtcctcgag catctcgatg
     4381 acatcgacga cgtcgcagcc gagctgggcg ccgtcaacac ggttgtcttc gacggcccgc
     4441 agactgtcgg atacaacacc gacacaacgg gattcgcact cggattccgt gaaggcctgc
     4501 ccgatgccgc caccgacaac gtggtgctcg tcggcgccgg cggtgccgga gccgctgtcg
     4561 gcgacgcatt gttgcggctg ggcaccgacc acctgacgat cgtcgatctc gatatcgaac
     4621 gcgccaccgc gctggcccgt gagttcgccg cgcgccgtcc cgactcacga gtggacgcgt
     4681 cctcgccgga caagctgcct gtcctgctgc ccggcagcga tggagtcgtg cactgcacgc
     4741 cgacggggat ggccgagcac ccaggtatgc cgttcgccac cgagttgctg catccgggcc
     4801 tgtgggtggc cgacatcgtc taccgcccgc tcgacaccga actgctgacc gcagcgctgc
     4861 aggtcgggtg ccgagtctgc gacggcggcc acatggccgt gcaccaggcc accgaggcct
     4921 tcgagctgat cactggtatc acccccgata ccgaacgcat gtccaggcac ttccgccgcc
     4981 ttgtcggctg aataaccacc accaccaaga agaaagagaa tcatgagcgg catcgaaacc
     5041 acgaaacctg atggggttgg cgaccattcg ccgcgccgca cgccaggccg cgcagcgctg
     5101 gccagcttca tgggcagcgc cgttgagtac tacgacttct tcgtgttcgg caccgccgcg
     5161 gcgctgatct tcccgaaggt gttcttccct tcgggtgacg aagctgcact ggtgatgtcc
     5221 ttcgccacat tcggtgtcgc atacgtcgcg cgcccggtcg gcgcattcgt cctcggacat
     5281 ttcggcgacc gcatcggtcg tcggaacgtg ctgatgttca ccctcgtgct gatgggtgtg
     5341 tcgaccttcc tgatcggctg tttgcctacg ttcgccactg ccggctggat cgcacctggg
     5401 cttttggtcg tctgccggtt aatgcaaggt ttctccgctg ccggtgaaca ggcgggcgcc
     5461 agttcactca cccttgagca cgcgccggat caccgccgcg cattcttcac gtcctggacg
     5521 ctgaccggca cgcagggcgg tctcatcctg gcgcagctgg tcatgctgcc gttcgtgtcg
     5581 ctgcccgacg acgccgagct ctcctggggt tggcgagttc cgttctggct cagcgctgtt
     5641 gtcgtcgtgg tggcctactt cattcgccga cgactgcatg agacacctga gttcgacgag
     5701 gccaaggcga ccggggcgat cgcgcggatg ccgctgaagc cgctgctgca acaccattgg
     5761 aaagacgtgc tgcgggtgat cttctgcgcg ttcatcgccg ccgtgtcaac ggttttcgga
     5821 actcttgcca tcgcctacgg caagaaagta gggctcgaag ccggcatcac gctgtggctg
     5881 gtcgtggtcg ccaatattgt cgcgctcttc actcagccgc tgttcggcat tctcgccgac
     5941 cggatcgggc gcaagccggt cttcatctac ggtgcgttgt cgtcggccgc gctgatgccc
     6001 ttctacatgc tgtcgatgag cggtgacaac acggtgctga ccttcgcgct ggccgtcgcc
     6061 acgttctcgt tcggatacgc cgcagcgaac gccgtatggc catcgttcta cggcgagatg
     6121 ttcagcaccc aggtgcggtt ctccggtatg gcgatcggca cgcagctcgg cttcctgctg
     6181 gccggcttcg cgcccagcat tgtcaccgcg ctcggcggtg tccgcgaagg cggctgggtc
     6241 gtcatcagtg ccttcaccgc ggtcgtctgt gtcatcgccg ccacgtcggc atccaccgca
     6301 cgcgagacca agaacgtccc caccccgctg ctcgggcgcg acctgcccga ctcacagaag
     6361 gttctggtca accgctgacc atggggctgc cctgcgggtc acagcagccg gccgatgccg
     6421 cgcgccgcga cctgcagggc gcccagcagc cgttggcggt cgcgtttgag ggtggccacc
     6481 accaccccga tcgcggccgc cacgctgtcg tcggaggccc gcaccacggg caccgccagc
     6541 gaacacgcac ccaacgacat ctcctcgctc gtcgtggcga ctccgtcgcg acggatccga
     6601 tccaactggg cgtcgagcac ctctgggtgg gtgatggtgt acggcgtgat ccgggtcagc
     6661 ttggcaaaca cccggtggtg cacgtcggac ggggcgtggg cgagcagcac cttcccgacg
     6721 ccggtggcgt gcatcggcag ccgggtaccc acagtgctga cgatcggcac cgacgcccga
     6781 ccccgcatcc ggtgcaaata cagcacctcg tcgccgtcgc gtacggccag gtggacggtt
     6841 gccaacgtgg ccgcgtacac gtcatgcaga aaaggctcag ccacctgccg caatcgccct
     6901 tgcacgggcg ccagcagacc ggcctcccac agcaggcggc cgatgacgta gcggccgtcg
     6961 tccagccgat gcagcgcgcc accggcaacc aactcaccaa ccagccgatg cgcggtcggc
     7021 accggtatca cggcgcgtcg cgcgagctcg gtcagggtca ggctgcgatg tccctcgtcg
     7081 aacgcagcga gcaccgacag caaccgcgat gtgaccgtgg ctcccggctc cgacgtgttc
     7141 ccggccaatc gtccaatcct tccgctgagc gaaagtctag ccttctcgac ctcgactcgg
     7201 agcgatagcg tgcgacacat gacagcaatc gtcgacacca atcccgacag cgcgtcggcg
     7261 agtcaggccg ggatatccgc cgagatcgcg gcggtcgaag ccgattacca ccgcgccggc
     7321 atcgaggaga ctcagccgcg gctgaactac ccgccgtacc gcagcagcct gctgcgccac
     7381 ccgaccaagg agttgcacca cgccgatccc gaggcggtcg aactgtgggc accgtgcttc
     7441 ggcgccggcg acgtcgcggc cgtcgagtcg gatctgacga tccaacacgg cggcgagccg
     7501 atcggcgaac gcatggtggt gaccgggcgt gtcatcgacg gtgacggccg gccggtgcgt
     7561 cgtcaactcg tcgagatctg gcaggccaac gccagcggcc gctacgcgca taagcgcgat
     7621 cagcatccag caccgctcga tcccaacttc accggtgtcg gacgatgcct aaccgacgac
     7681 gacggcacct accgcttcac cacgatcaaa cctgggccgt atccgtggaa gaaccatcac
     7741 aacgcgtggc gacccgcgca cattcatttc tcgttgttcg gaacggattt cacgcagcgg
     7801 atgatcacgc agatgtactt cccgggtgat ccgctgttcg cgctggaccc gatttaccag
     7861 tccatcaccg accagaaggc ccgcgaccgg ctcgtcgcgg cctacgacca tgacgtaacc
     7921 acgcacgaat gggctaccgg ctatcggtgg gacatcgtgt tgaccggaag cgcacgcact
     7981 ccgatggagg ccgaatgagc accttgacgg ctacccccgg tcagacgatc gggccgttct
     8041 tcggttacgc cctgccgttt tcgcgcggga acgaactcgt ccaacccggc tcggccgggg
     8101 cgatccggct gcacggcgtc gtcaccgacg gcgagggtgc gcccgtaccc gacgccctgc
     8161 tggagatctg gcaagccgac gccgacggtg tggtcccgtc ggccaccgga tcgctgcgcc
     8221 gcgacggctg gaccttcacc gggtggggga gggccagtac cgacgacgac ggccactaca
     8281 gcttcaccac ggtcaaaccc ggtgctgtac agcctgattc ggcgccgttc atcgccgtga
     8341 ccgtattcgc gcgtggcctg ctcaaccggc tcttcacccg cgcatacgtg ccgagagacc
     8401 ggctcgcgca ggaccggctg ctgagcacgg ttcccgcgga ccggcgtcac acactgatcg
     8461 cggtgcccgg cgagcacggc ttgcgcttcg acgtccacct tcaaggccag ga
//
LOCUS       DASRJA010000593         2612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3927072,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000593 DASRJA010000000
VERSION     DASRJA010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3927072"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VFC01_26360"
     CDS             <1..1330
                     /locus_tag="VFC01_26360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83173.1"
                     /translation="KAKPAGVVTSTLQFAPNGGLTEFTVTATSWVRTPFLGVLHAWAK
                     HSGNDAAPDGCKANYFGCVGLTSTATAALCPSTSCIGTSSGGSNIEVSLMLDVTGSMC
                     SPCTKIDALKKAAKDLIDIVVWDDQSQYTSRVALAPFADAVNVGTTLAPLVRGTTISN
                     TSATAEAFTTTSVLNDVTKQPTKKWIKFNSVNGLNSGGCTTSGTDNCTWQIHTKCVTE
                     RVGTHAYDDTAPVAATANTLVGKGYFSDSATSGCSVAANADPEVNLIMPMTKDKTALK
                     ARIDKLTTGGSTAGHMGTAWAYYLLSPKWNSLFPAASAAGPYSDLTVYNSKGMPKLRK
                     IAVLMTDGEYNINYCKGVEAQNSDQTPDIKCNSENGKSLVQAASMCAVIKSAKIEVFT
                     VGFQVNSTAKTFLTNCATDASHYYDATTEVALQAAFRDIALKIATLRLTN"
     gene            complement(1709..>2612)
                     /locus_tag="VFC01_26365"
     CDS             complement(1709..>2612)
                     /locus_tag="VFC01_26365"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HZN83174.1"
                     /translation="EAGINQLAGTQTANRVVLTVGKFAVTDVFDGNKYAHDARSDFLN
                     WALIDTATFDYASDAWGYTYGAAVEWYRGPWTLRGGFFDLPITPNNTQLDPSFAQNQW
                     VGEVERRYKLWDQPGKLAITGYLTHGRMGTFADATRLALATGEPADIAAVRRVQNQSG
                     IGFNLEQQLFPELGLFARGGLAGGKKEPFAFTDVDRTLAAGLVVSGKLWGRPDDSIGL
                     GGVVNGITGQHQAFFNAGGLGILVGDGKLPNPGPEKILELYYSLPLLASARLTLDYQF
                     IDNPGYNQDRGPVSVIATRLRAHF"
BASE COUNT          501 a          835 c          860 g          416 t
ORIGIN      
        1 caaagccaag cccgccggcg tcgtcaccag cacgctgcag ttcgcgccca acggggggct
       61 gacggaattc accgtcacgg ccacctcctg ggtcaggacg ccgttcctcg gcgtgctgca
      121 tgcgtgggcc aaacattccg gcaacgacgc ggcacccgac ggctgcaagg ccaactactt
      181 cggatgcgtg ggcctcacga gcacggccac cgccgcgctc tgcccgagca cgtcctgcat
      241 cggcaccagc agcggcggca gcaacatcga ggtgtcgctg atgctcgacg tcacgggctc
      301 gatgtgcagc ccctgcacga agatcgacgc cctcaagaag gccgccaagg acctgatcga
      361 catcgtggtc tgggacgacc agagccaata cacgtcgagg gtggcgctgg cccccttcgc
      421 ggacgccgtg aacgtcggca ccacgctggc accgctggtg cgcgggacca cgatctccaa
      481 cacctccgcc accgccgagg ccttcaccac gacgtccgta ttgaacgacg tgacgaagca
      541 gccaacgaag aaatggatca agttcaacag cgtcaacggc ctcaacagcg gcggctgcac
      601 caccagcggc acggacaact gcacctggca aatccacacc aagtgcgtga ccgagcgcgt
      661 cggtacccac gcctacgacg acactgcgcc ggtcgcggcg acggccaata ccctcgtcgg
      721 caagggctac ttcagcgaca gtgcgacctc gggatgctcc gtggcggcca atgccgatcc
      781 cgaggtcaac ctgatcatgc ccatgacaaa ggacaagacg gcgctcaagg cacgcatcga
      841 caagctcacc accggcggct cgacggcggg ccacatggga acagcctggg cttactatct
      901 gctgtcgccc aagtggaatt ccttgtttcc ggcggccagt gccgcggggc cctacagcga
      961 cctgacggtc tacaactcca agggcatgcc caagctgcgc aagatcgccg tgctgatgac
     1021 ggacggcgag tacaacatca actactgcaa gggcgtggaa gcgcagaact cggatcagac
     1081 tcccgacatc aaatgcaact cggagaacgg caagtcgctg gttcaggcgg cgagcatgtg
     1141 cgcggtgatc aagagcgcca agatcgaggt gttcacggtc ggcttccagg tgaattccac
     1201 ggccaagacg ttcctgacca actgcgccac cgacgccagc cactactacg acgccaccac
     1261 cgaagttgcg ctgcaggcgg ccttccgcga catcgcgctg aagatcgcga cgctgcggct
     1321 gacgaactga gggcggcgag ccggggcggc ggagaagggc ggcagagatg ccgtcctttg
     1381 cgttggggag ctgacgcatc tgtggccaca ccatcgctgg tcgtccaacc agacattcac
     1441 aggcccgcac atacgcgttc gggttctgca aaccatcagc gccacacatg tcatcctcgg
     1501 acttggtcag acttggtccg aggatccatc gcgctgcatg ctccctacca ctagggtcgg
     1561 cgatgcggag gaagatccat cgatcgacgc atggtcgtcc tgttcccgag ttgtgcggtg
     1621 tgttgcccca gttcaaagct cgacgttcgc ggaactctgg atcctccgag gatgacaagt
     1681 cgagtgcgcg gcgtcgtcgc gtgccgcatc agaagtgcgc gcgcaggcgg gtggcgatga
     1741 cggagacggg gccgcggtcc tggttgtagc cggggttgtc gatgaattgg tagtccagcg
     1801 tcaggcgcgc ggaggcgagg agcgggaggc tgtagtagag ctcgaggatt ttctcgggtc
     1861 ccggattggg cagcttgccg tcgccgacga ggatgccgag gccgccggcg ttgaagaacg
     1921 cctggtgctg gccggtgatg ccgttgacga cgccgccgag gccgatcgag tcgtcgggac
     1981 gcccccacag cttgccggag acgaccaggc cagcggcgag cgtgcgatcg acgtcggtga
     2041 aggcgaacgg ctccttcttg ccgccggcca ggccgccgcg cgcgaacaac ccgagttccg
     2101 ggaagagctg ctgctcgagg ttgaagccga tgcctgactg gttctggacg cggcggacgg
     2161 cggcgatgtc ggcgggctcg ccggtcgcca gcgcaagccg cgtggcgtcg gcgaaggtgc
     2221 ccatgcggcc gtgggtgaga tagccggtga tggcgagctt gccgggctga tcccacagct
     2281 tgtagcgccg ctcgacctcg cccacccatt ggttctgcgc gaagcttggg tccaactggg
     2341 tgttgttggg ggtgatggga agatcgaaaa aaccgccgcg cagggtccaa ggtccccggt
     2401 accattctac cgcggctccg taggtataac cccacgcatc cgaggcgtag tcgaacgtcg
     2461 cagtgtcgat gagcgcccaa ttgaggaagt cggagcgtgc gtcgtgtgca tacttgttgc
     2521 catcgaagac gtcggtgacc gcgaacttgc cgaccgtgag caccacacgg ttggcggtct
     2581 gtgtgccggc gagctgattg atgccggcct cg
//
LOCUS       DASRJA010000594         5456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3903483,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000594 DASRJA010000000
VERSION     DASRJA010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3903483"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFC01_26370"
     CDS             complement(<1..447)
                     /locus_tag="VFC01_26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN83175.1"
                     /translation="MSVLIRGVRLYGEGDRVDVLASDGQIADIGADLAAPDDADVFDA
                     TDQILLPGFVDLHTHLREPGREYAEDIETGSAAAALGGYTAVFAMANTNPVADSPVVT
                     DHVWHRGQQVGLVDVHPVGAVTMGLAGAQLTEMGMMAAGAAQVRMFS"
     gene            complement(444..1391)
                     /locus_tag="VFC01_26375"
     CDS             complement(444..1391)
                     /locus_tag="VFC01_26375"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZN83176.1"
                     /translation="MKHLLSAGDLSRDDALAILDDADRFSQALLGREVKKLPTLRGRT
                     IITMFYENSTRTRVSFEVAGKWMSADVINVSASGSSVSKGESLRDTALTLRAAGADAL
                     IIRHPASGAAQQLAEWTATEHGGPVVINAGDGTHEHPTQALLDALTIRQRLGTIEGKR
                     VVIVGDVLHSRVARSNVLLLATLGAEVVLVAPPTLLPVGVADWPVTVSHDLDAELPIA
                     DAVLMLRVQAERMNGGFFPSAREYSVLYGLSEKRQAMLPGNAVVLHPGPMVRGMEIAF
                     SVADSTQSAVLQQVSNGVHVRMAVLFHLLVGAETEAISA"
     gene            complement(1388..1972)
                     /gene="pyrR"
                     /locus_tag="VFC01_26380"
     CDS             complement(1388..1972)
                     /gene="pyrR"
                     /locus_tag="VFC01_26380"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HZN83177.1"
                     /translation="MGSNAASSGTDRELMSAADVGRTISRIAHQVIEKTALDGPDAPR
                     VILLGIPTRGVTLAARLADKIKEFSGVTVPHGALDITLYRDDLDIKPPRPLEDTSIPE
                     GGIDNALVVLVDDVLYTGRSVRSALDALRDIGRPRAVQLAVLVDRGHRELPLRADYVG
                     KNVPTSRSENVKVRLSEDDGHDGVSIAPHGGPAR"
     gene            complement(2084..5443)
                     /locus_tag="VFC01_26385"
     CDS             complement(2084..5443)
                     /locus_tag="VFC01_26385"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZN83178.1"
                     /translation="MINTIQIDTAPETNYHTLAVRADGALVVTDLADRALRRVTYSRG
                     NTAMVVIANPTVGEPDSSTGAVTGLVNIKDWDGDPLTYTPLLAPSKGSVTYDASAGTY
                     TYTPTQAAREQAGPSTVDQFSVRVSQGQASEDVSVTVPVKPVDVNRSPQAGNPSVGTP
                     DRSTGAVSGSLNFYDPDEDPLTYNVPVQPASGTVTITGSTYTYTPTQAARDAAAASPD
                     QDLGTITVFASDGQASTSKTFTVPIAPTPPPNTAPTASPTQAIPNQTTGAITGAVNGA
                     DVDGNTLSYSLAGAPPASGFVTLNSATGAFTYMPTVLARIAAHDKSAGPDFDSFTVAV
                     SDGQASTSVTVQVAVLPSLIPFISNTAQTGSNPMGITVSPTKVYVANQLSNSVSVIDR
                     ANPTATPVTINVVAAPRAIALSSDGTRAYVAGNGGVSVINLATNQLITTVATSAGDSY
                     GIAVTPTVNGQHRVYVTNSTTNTVRVIDANITANTYTAGASVTVGSGPRGIAVSPDGS
                     RAYVTNWTSNSVSVLDTSTATPALVGSPIAVGTNPVGVVVSPNGARVYVSNYGSASVS
                     VLNPTAATPLVTTITVGAQPFGMAITPDGSIVYAANSFDTVSMIDTKTNSVYSTLTID
                     DAQPEQQWHSVAVSPDGRQVYISDLADRTVRVATMIRSNSTPTLSTSIVSRDSITGVV
                     SGVVNAKDYDGDSVTVAVVSGPSKGSLTYNASTGAYTYTPSQAARDQAAQTPGITDTF
                     TVRATDSFGGFNSSSSINVTVPVSPNLYLNLPAGVTPIVVGTNPIDMTVAGTRLFVLN
                     SGDGSVSVIDTATKQVVKTIAGLGYSSPMVASKDGRYLYASQYDWYSATASVKVVDTA
                     TGGVVATVFMPKCETEGCDLPGGGWANSAGITDIAVSPDGTRVYVSEVWVGDTFYGGT
                     LTMIDTSTNTVVGSVQPSQWGDFYSNIEVSPDGTRIYAGSGYGYVPRMDVLDARTLAG
                     IGYVPLAGNTGWPPPSTGSLTFSSNGERAYVRTTQMWPTYSSQTFAVIDTKLGSPTYN
                     TQIATITVPAGAQYMIVSPDNSRGYVVHEGGKLFTIVDLTTNTVIRSVTSDQVGGDYA
                     ALAIGSDGTLYFTNYPKNAVYAVAPGSPLVV"
BASE COUNT          935 a         1655 c         1845 g         1021 t
ORIGIN      
        1 ggagaacatc cgaacctggg ccgcgcctgc ggccatcatg cccatctcgg tcaactgcgc
       61 tccggccaga cccatggtca ccgcgccgac gggatgcacg tcaaccagcc cgacctgctg
      121 gccgcggtgc cacacgtggt cggtgacgac ggggctgtct gcgaccgggt tggtgttggc
      181 catcgcgaac acggccgtgt aaccacccaa agccgctgca gcagagccgg tttcgatgtc
      241 ctcggcgtac tcccggccgg gctcccgcaa gtgggtgtgt aggtcgacga agccgggaag
      301 caggatctgg tcggtggcgt cgaaaacgtc ggcgtcgtcg ggtgccgcca ggtccgcgcc
      361 gatgtccgcg atctggccgt cggaggcgag cacgtcgacc cggtcgccct ccccgtagag
      421 ccgcacccct cgaatcaaga cgctcatgcg ctgatcgcct ccgtctccgc accgacgagt
      481 aagtggaaca gcacggccat ccggacgtgt acgccgttgg aaacctgttg caggactgcc
      541 gattgcgtcg aatccgcgac cgagaaggcg atctccatac cgcgcaccat cgggccgggg
      601 tgcagcacga ccgcgttgcc gggcagcatc gcctggcgct tctcggagag cccgtagagc
      661 acggagtact cccgggccga cgggaagaag ccgccgttca tccgctcggc ctggacgcgg
      721 agcatcagca ctgcgtcggc gatgggcagt tcggcgtcga gatcatgtga gacggtgacc
      781 ggccaatcgg ccaccccgac cggcagcagt gtgggcggcg cgacgagcac cacttcggcg
      841 ccgagagtgg cgagcagcag gacattggac cgcgcgaccc tgctgtgcag cacgtcgccg
      901 acgatcacca cccgcttgcc ctcgatggtg cccaaccgtt gccggatggt cagcgcgtcg
      961 agcagcgcct gggtcggatg ttcgtgcgtg ccgtcgcctg cgttgatcac caccggcccg
     1021 ccgtgttccg tcgccgtcca ctcggcgagc tgctgggccg ccccggacgc cgggtggcgg
     1081 atgatcagcg cgtcggcgcc agccgcccgc agtgtcaacg cggtatcgcg cagcgattca
     1141 cctttggaca ctgaggatcc cgacgcgctg acgttgatga cgtcggcgct catccacttg
     1201 cccgcgacct cgaacgacac ccgggtgcgg gtggagttct cgtagaacat cgtgatgatg
     1261 gtgcggccgc gaagagtcgg cagcttcttc acctcgcggc cgagcagcgc ctggctgaac
     1321 cggtcggcgt cgtcgagaat ggccagcgcg tcgtcgcggg acaggtcgcc ggccgaaagt
     1381 agatgcttca tcgtgcgggc cctccgtgag gtgcgatgga tacgccgtcg tggccgtcat
     1441 cctcggagag gcggaccttg acgttctcgc tgcgcgaggt gggcacgttc ttgccgacgt
     1501 agtcggcgcg caggggcagc tcgcggtgac cgcggtcgac gaggaccgcg agctgcacag
     1561 cgcggggccg accgatgtcg cgcagcgcgt ccagggcgga gcgcacggag cgaccggtat
     1621 agaggacgtc gtcgacgagg acgaccagcg cgttgtcgat gccgccttca gggatcgagg
     1681 tgtcctcgag cggccggggc ggcttgatgt cgaggtcgtc gcggtagagc gtgatgtcca
     1741 gcgcgccgtg cggaaccgtg acgccggaga attctttgat cttgtcggcc agccgggcgg
     1801 cgagggtgac gccgcgggtc gggatgccga ggaggatgac gcggggtgcg tcgggaccgt
     1861 cgagtgcggt cttttcgatg acctgatggg cgatgcggga gatggtgcgg ccgacgtccg
     1921 ccgcggacat cagctcccgg tcggtgcccg aagaggctgc gttagagccc aagtgaccct
     1981 gacctccttc tccgcctctc tggacggatc gttaaaggac gtcgaacttc cgggcgagct
     2041 tagcacctcg ttctgcagtg gctttcggcg acaccgcctc gtttcagacc accagggggc
     2101 tacccggggc gacggcgtag acggcgttct tcgggtagtt ggtgaagtac agcgtgccgt
     2161 cggagccgat tgcgagggcc gcgtagtcac cgcccacctg gtcagaagtg accgacctga
     2221 tgacggtgtt ggtcgtcagg tcgacgatgg tgaacaactt gccgccctcg tgaacaacgt
     2281 agccgcggct gttgtcgggg ctcacgatca tgtactgcgc cccggcgggg accgtaatcg
     2341 tcgcgatctg ggtgttgtag gtcgggcttc ccagcttggt gtcaatcacg gcgaaagtct
     2401 gggacgagta tgtaggccac atttgggtcg tccgcacgta tgcgcgttcg ccgttcgaac
     2461 tgaaggtcag gctgcccgtc gacggcggcg gccaccccgt gttgccggca aggggcacat
     2521 agccgatacc ggcaagggtg cgcgcgtcga ggacgtccat tcggggcacg taaccgtagc
     2581 cacttcccgc gtagatccgg gtgccgtcgg ggctcacctc gatgttcgaa tagaaatcgc
     2641 cccattgcga gggttgaacc gacccgacga ccgtgttggt agaagtgtcg atcatcgtga
     2701 gggtgccgcc gtagaaggtg tctccgaccc acacctcgga cacgtacacc cgggtgccgt
     2761 cggggctgac cgcgatatcg gtgattccag cggagttggc ccagcccccg ccgggcaaat
     2821 cgcagccctc ggtttcgcac ttgggcatga agacggtggc cacgacacca ccggtagcgg
     2881 tgtcgaccac cttcactgag gcggtcgcgg agtaccagtc gtattggctg gcatacagat
     2941 accgaccgtc cttggatgcc accatgggcg acgagtatcc gagcccggcg atggtcttca
     3001 ccacctgttt ggtggcggta tcgatcactg acacgctgcc gtcgccgctg ttgaggacaa
     3061 agagtcgagt tccagcgacc gtcatatcga tcggattggt acccaccacg atcggtgtca
     3121 ctcccgcggg caggtttagg tacaggtttg gtgataccgg cacggtgacg ttgatgctgc
     3181 tgctgctgtt gaagccaccg aacgaatccg ttgctcggac agtgaaagta tcggtgattc
     3241 ccggtgtttg ggccgcttgg tcgcgagcgg cctggctggg ggtgtaggtg tacgcgccgg
     3301 tggacgcgtt gtacgtcaag gatcccttgc tcggtccgct gacgacagcg acggtgaccg
     3361 aatccccgtc gtagtccttg gcattcacga cgccgctgac tacgccggtg atggaatcgc
     3421 gggagactat cgatgtggag agcgtcggag tgctgttgct gcggatcatc gtggcgaccc
     3481 gcacggtgcg atcggccaga tcagagatat atacctgtcg tccgtcgggg gacacggcga
     3541 ccgaatgcca ttgctgctca ggttgagcgt catcgatggt caacgtggaa tacacgctgt
     3601 tggtcttggt gtcgatcatc gacactgtgt caaagctgtt cgccgcgtac acgatgctgc
     3661 catcgggggt gatggccatt ccaaatggct gagcgcccac cgtgatagtg gttaccaacg
     3721 gcgtcgcagc ggtcggattg agcaccgaca cactggcaga gccgtagttg gacacgtaga
     3781 cccgcgcgcc gttcggcgac acgacgacac caacgggatt ggtgcccacg gcgatcggcg
     3841 atcccaccag cgcaggcgtc gccgtgctcg tgtcgaggac ggagacactg ttggaggtcc
     3901 agttcgtcac ataggcgcgg ctaccgtccg gactgacagc aatccccctt ggaccactac
     3961 ccaccgtcac cgaagcgccg gcagtgtagg tgttcgcggt gatgttggca tcgatgactc
     4021 gcacggtgtt ggtggtggag ttggtgacgt acacccgatg ctgcccattg acagtggggg
     4081 tcaccgcgat tccatatgaa tcccccgcgc ttgtggccac agtcgtaatc aactgatttg
     4141 tggcgaggtt gataaccgac actccgccgt tacccgctac atacgcgcgc gtcccatccg
     4201 aactcagcgc gatcgcccgt ggtgcggcga ccacattgat cgtgacgggc gtcgccgtcg
     4261 gattggctcg gtcgatcacc gaaacagaat tcgacagctg attggccaca tacaccttcg
     4321 tcggcgagac cgttatcccc atcgggttgc tgccagtctg cgcggtgtta ctgatgaacg
     4381 ggatcagcga gggcaacacc gcaacctgaa ctgtcaccga cgtcgaagct tgaccgtcgg
     4441 aaaccgccac agtgaaactg tcgaaatcgg ggccggcgct cttgtcgtgc gccgcgattc
     4501 gcgccaatac ggttggcatg tacgtgaacg cgcccgtggc cgaattcaac gtcacgaatc
     4561 cactcgcagg cggcgcgcca gccaggctat aggacaatgt attgccatcc acatcagcac
     4621 cattgacagc acccgtgatc gcaccggtgg tctggttggg tatcgcctgc gtcggcgaag
     4681 ccgtcggcgc agtattgggt ggcggtgttg gtgctatcgg cacggtgaac gtcttggatg
     4741 tggaagcttg accatcactg gcgaacacgg tgatggtgcc cagatcctga tctggggacg
     4801 cggcggcggc gtcgcgcgct gcctgggtag gtgtgtaggt gtaagtcgac ccggtgatgg
     4861 tgaccgtgcc gctggccggt tgcaccggca cgttgtaggt gagcggatct tcatcggggt
     4921 catagaaatt caacgatccc gacaccgctc ctgtgctccg atctggcgtt ccgactgacg
     4981 ggtttcctgc ctgcggtgac cgattcacat cgaccggttt gacaggaacg gtgacgctga
     5041 catcttcgga tgcttggccc tgactgacgc gcacggagaa ctggtcgacg gtactgggcc
     5101 cagcctgctc acgggcggct tgcgtcggtg tgtacgtata ggtgcccgca ctcgcgtcat
     5161 aggtgacaga tcccttggaa ggagcgagaa gaggggtata ggtcagtgga tcgccgtccc
     5221 agtctttgat gttcaccaac ccggtaacgg caccggtcga tgagtccggc tctccgacag
     5281 tgggattggc gatcactacc atcgcggtgt taccgcggct gtaggtgacc cggcgcagcg
     5341 ctcggtcggc caggtcggtg accaccagag cgccatcggc gcgcaccgcc agggtgtggt
     5401 agttggtctc cggtgcggta tcgatctgaa tggtgttgat caccgcacga gtcttg
//
LOCUS       DASRJA010000595         7744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_540604,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000595 DASRJA010000000
VERSION     DASRJA010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540604"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFC01_26390"
     CDS             <1..807
                     /locus_tag="VFC01_26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN83179.1"
                     /translation="SEMTWLIADELVQIRGGRGFETAESQAARGERGVPAEQALRDVR
                     INRIFEGSTEIMHLLIAREAVDVHLSVAGDIIDPDADMRRKARAAANAGRFYARWLPS
                     LLAGKGQLPTSFGEFGPLGAHLRYVERASRRLARSTFYGMSRWQGKLEHKQRFLGRIV
                     DIGAELFAMTAACVRAQRDRTEGAAVELADAFCRQARVRADRLFDELWRNSDDTDRTL
                     ARSVLDNRYTWLEEGILDPSIEGPWIAQDGGAAESDVHRFIRTVTRRGVA"
     gene            824..1168
                     /locus_tag="VFC01_26395"
     CDS             824..1168
                     /locus_tag="VFC01_26395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83180.1"
                     /translation="MNSQIRPLEDVASCEIPYWLPPGGRDNGVWADIWVLIADLESSD
                     ATEVLDLLAKADVGGYAAVPGGIRARARRPVRCGVWVDAMQYGIAEDVLIRFMRDRSA
                     RERGARAGNGCR"
     gene            1194..2138
                     /locus_tag="VFC01_26400"
     CDS             1194..2138
                     /locus_tag="VFC01_26400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HZN83181.1"
                     /translation="MSAGLFGLLDDIAALARLAAASVDDIGAAAGRATAKAAGVVVDD
                     TAVTPQYVHGIAAERELPIIKRIAIGSLRNKLVFILPAALLLSQFVPWLLTPILMVGA
                     TYLCYEGAEKVWGRFNKHGGHDHSQPTTTLGEDAEKQMAAGAIRTDFILSAEIMVIAL
                     NEVANESFWPRLIILAVVAVVITIAVYGVVGAIVKMDDIGLSLTQRSSRFAQRIGRGL
                     VAAMPKLLSALSAIGTVAMLWVGGHILLVGTDDVGWHAPYGLVHHAEDFVHHAVQGVG
                     ALLAWLVNTAASAAVGLVVGAVVVAIMHVLPFGRKNAH"
     gene            complement(2135..3361)
                     /gene="manA"
                     /locus_tag="VFC01_26405"
     CDS             complement(2135..3361)
                     /gene="manA"
                     /locus_tag="VFC01_26405"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HZN83182.1"
                     /translation="MHLLRGAVRTYAWGSRTAIAEFTGRPSPTAHPEAELWFGAHPGD
                     PAWLETDGGERSLLDTLRKDPEGQLGAAVRGRFGDCLPFLVKVLAADEPLSLQAHPSA
                     QQAVEGFAREDKLGIPVNSAIRNYRDRSHKPELLVALGQFEALAGFRPAASSVELMHA
                     LAVSDLDPFVNLLSGQSDADGLRALFTTWITAPQPDLDVLVPAVIDGAIHYLRSGEKK
                     FAAEAKTVLELGERYPGDAGVLASLLLNRLSLKPGEAIYLPAGNLHAYLHGVGVEVMA
                     NSDNVLRGGLTPKHVDVPELLRVLDFTPATDDVIYPRITRDGAELVYNTPAPEFAVSV
                     LCIEGEHLGHEIDAPTRHDGPQILLCTEGSTVVHAKSTAVTLERGAAAWVAADDGPIR
                     LVAQTPTKLFRATVGI"
     gene            complement(3366..4460)
                     /locus_tag="VFC01_26410"
     CDS             complement(3366..4460)
                     /locus_tag="VFC01_26410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TobH protein"
                     /protein_id="HZN83183.1"
                     /translation="MNATHAAVDLGDVEGLLAADRDGLLRAASMAGAQVRATAAALDE
                     GDLEPLKSDQPPRTVVWVSGRGTAETAGAMLSATLGGSVGSPIVVASEVPPWIGALDV
                     LILAGDDPGDPALVDAAATAVRRGARIAVVAPYEGPLRDATAGRSVSLAPRLRVRDDF
                     GLSRYLAAGLAVLQVVHAGLQVDLAALADELDAEAFRNSAARELFTNPAKTLADRMLG
                     RDIVLAGDTVATLTLARHGAAIMLRIAHQSVAAVGLADALVALHGGIAGLSGVDHEKS
                     IFHDEQIDGPLPKRLRTFVLATDAERPVVVARVSGLDDVDVINAEDVPEMSDAPETVV
                     ADATGRPEQQLAMLAVRLEMTAVYLRLVRG"
     gene            complement(4457..5854)
                     /locus_tag="VFC01_26415"
     CDS             complement(4457..5854)
                     /locus_tag="VFC01_26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZN83184.1"
                     /translation="MSRPAAAVQRVIKAYDVRGLVGEELDEGFVADVGGAFARLVRDG
                     ASQVVIGHDMRASSPSLAAAFAEGVMAQGLDVVRIGLASTDQLYFASGLLDCPGAMFT
                     ASHNPAAYNGIKLCRAGAKPVGKETGLTAITEDIIAGVPAFDGSRGTICDRDVLADYG
                     EFLRSLVSLSELRPLRVAVDAGNGMGGHTTPAVLGVIPSITLLPLYFELDGTFPNHEA
                     NPLEPANLVDLQAYVVETGADIGLAFDGDADRCFVVDEKGHPVPPSAVTALVAARELN
                     REIGATVIHNLITSRAVPELVAERGGTPVRSRVGHSYIKTLMADTGAIFGGEHSAHYY
                     FRDFWGADSGMLAALHVLAALGEQDRPLSELMADYQRYEASGEINFTVTDAQGCVDAV
                     LKSYGSRIHSIDHLDGVTVDLGEGSWFNMRMSNTEPLLRLNVEARTAEAVDEIVGQVS
                     RLVGTQNPSESKAAT"
     gene            complement(5929..6348)
                     /locus_tag="VFC01_26420"
     CDS             complement(5929..6348)
                     /locus_tag="VFC01_26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 domain-containing protein"
                     /protein_id="HZN83185.1"
                     /translation="MNVPRRCCRPGCPHYAVATLTFVYSDSTAVVGPLATVSEPHSWD
                     LCVAHAGRITAPRGWELVRHAGPLPSHPDEDDLVALADAVREGRDGSPTFNGVAAGFS
                     DPATGVHGGALMAPAAPRPESNGRRRGHLRVLPDPSD"
     gene            6506..6928
                     /locus_tag="VFC01_26425"
     CDS             6506..6928
                     /locus_tag="VFC01_26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HZN83186.1"
                     /translation="MRGPLLPPTVPGWRSRAERFDMAVLEAYEPIERRWQDRVSGLDV
                     AVDEIPRISPKDPDAVQWPPEVLADGPIALARLIPAGVDVRGNATRARIVLFRKPIER
                     RAKDTVELAELLHELLVAQVATYLGVEPSVIDPTIDDE"
     gene            complement(6938..7207)
                     /locus_tag="VFC01_26430"
     CDS             complement(6938..7207)
                     /locus_tag="VFC01_26430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HZN83187.1"
                     /translation="MVPAQPERYEVAPATTEDDQWQERALCAQTDPEAFFPEKGGSTR
                     EAKRICLGCEVRDRCLEYALAHDERFGIWGGLSERERRRLKRGII"
     gene            7631..>7744
                     /locus_tag="VFC01_26435"
     CDS             7631..>7744
                     /locus_tag="VFC01_26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HZN83188.1"
                     /translation="MKVTVLVGGVGGARFLLGVQRLLGLGRFSGQSGTSPFV"
BASE COUNT         1310 a         2559 c         2599 g         1276 t
ORIGIN      
        1 tcagaaatga cctggctgat cgccgacgag ctcgtgcaga ttcgcggcgg tcgcggcttc
       61 gagacggccg agtcgcaagc tgcccgcggc gagcgcggag tacccgccga gcaagcgctg
      121 cgcgacgtgc gcatcaaccg gatcttcgaa ggctcaaccg agatcatgca tctgctcatc
      181 gcgcgcgagg cggtggatgt gcatctgagc gtggccggcg acatcatcga cccagacgcg
      241 gatatgcggc gcaaggcacg ggcggccgcc aacgccggcc ggttctacgc ccgctggctg
      301 cccagcctgc tcgccggaaa gggccaattg ccaacttcat tcggcgagtt cggaccgctt
      361 ggcgcgcatt tgcgctacgt cgagcgtgcc agccggcgac tggcgcgcag cacgttttac
      421 gggatgtcac gatggcaggg caagctggag cacaagcagc ggttcctggg caggattgtg
      481 gatatcggtg cggagctgtt cgcgatgacc gccgcctgtg tccgggcaca acgtgaccgg
      541 accgaaggtg ccgcagtcga actcgccgac gcgttttgcc gccaggcccg ggtccgggcg
      601 gaccggctgt tcgacgaact atggcgcaac tctgatgaca ccgaccgcac gcttgcgcgt
      661 agtgtgctcg acaatcgcta cacctggctc gaggaaggga tcctcgatcc gtccatcgag
      721 gggccctgga tcgcccagga cggtggcgcc gcggaatccg acgtacaccg cttcatccgt
      781 accgtcacgc gtcgcggcgt ggcgtaatgc tgcggatgcc gccgtgaatt cgcagatccg
      841 gccgctggag gacgtcgcat cgtgcgagat cccgtactgg ttgccgcccg gcggtaggga
      901 caacggcgtg tgggccgata tatgggtact gatcgccgat ctcgaatcca gcgacgccac
      961 tgaggttctc gacctgttgg cgaaggccga cgtcggtggc tatgccgcgg ttcccggtgg
     1021 gatcagagcc agggcacgac ggccggtccg ctgcggtgtg tgggtggatg cgatgcagta
     1081 cggcatcgcc gaggacgtgc tgatccggtt catgcgcgat cggtccgcgc gagaacgcgg
     1141 tgcgcgcgca ggcaacggct gcaggtagcc ttcggtgcca tgaggacgac gcgatgagcg
     1201 ccggtctgtt cggacttctc gacgacattg cggcgctcgc ccggctcgcg gccgcatcgg
     1261 tcgacgacat cggcgcggcc gccggtcgcg cgaccgccaa agcggcggga gtcgtcgtcg
     1321 acgacaccgc agtgacaccc cagtacgtgc acggcatcgc cgccgaacgc gagctgccga
     1381 tcatcaagcg gatcgcgatt ggctcactgc gcaacaagct ggtgttcatc ctgccggccg
     1441 cgctgctgct cagccagttt gtgccgtggc tgctgacgcc gatcctgatg gtcggtgcga
     1501 cgtatctctg ctacgagggc gccgagaagg tgtggggccg cttcaacaaa cacggcgggc
     1561 acgaccactc tcagcccacc acgacgctcg gcgaggatgc cgagaagcag atggccgcgg
     1621 gcgcgatccg caccgacttc atcctgtccg ccgagatcat ggtgatcgcc ctcaacgagg
     1681 tggccaatga gtcattctgg ccgcggctca tcatcctggc cgtcgtcgcc gtggtcatca
     1741 ccattgcggt gtacggcgtg gtcggcgcca tcgtgaagat ggacgacatc gggctgagcc
     1801 tgacgcagcg cagctcacga ttcgcgcaac ggatcggccg cggtctggtc gcggcgatgc
     1861 ccaaactgct gtctgcactc tcggccattg gcacagtcgc gatgctctgg gtcggcggcc
     1921 acattctgct cgtcggcact gacgacgtcg gctggcacgc accctacgga ttagtccatc
     1981 acgctgaaga tttcgtccac catgccgtcc agggcgtcgg cgcgctgttg gcctggttgg
     2041 tcaataccgc ggcgtcagcg gcggtcggct tggtcgtcgg cgccgtggtc gtcgcgatca
     2101 tgcacgtgct gcctttcggc aggaaaaacg cgcactagat cccgacggtc gcccggaaca
     2161 gcttcgtcgg agtctgcgcg accaaccgga tcggcccgtc gtcggccgcc acccacgcgg
     2221 cggcgccacg ctcaagtgtt accgcggtgg atttcgcgtg cacgaccgtc gaaccctcgg
     2281 tgcatagcag gatctgcggg ccgtcgtggc gtgtcggtgc atcgatttcg tggcccagat
     2341 gttcgccttc gatgcacagc accgacactg cgaattccgg cgccggcgtg ttgtacacga
     2401 gttctgctcc atccctggtg attcggggat agatgacgtc gtcggtcgcg ggcgtgaagt
     2461 cgagcacccg cagcaattcc ggcacgtcaa cgtgtttggg cgtgaggccg ccgcgtagga
     2521 cgttgtcgga gttggccatt acctcgacgc ccacgccgtg caggtatgcg tgcaggttgc
     2581 ccgcgggcag gtaaatggcc tcgccgggct tgaggctgag tcggttcagc agcagggagg
     2641 ccagtacacc ggcatcgcct gggtaacgct cgccgagctc gagcacggtc ttggcctcgg
     2701 ccgcgaactt cttctcgccc gaccggaggt agtggatcgc cccgtcgatc accgcgggca
     2761 ccagcacgtc gaggtcgggc tgcggggcgg tgatccacgt ggtgaacaac gcccgcagtc
     2821 cgtcggcatc ggactgaccg gacagcagat tgacgaacgg gtcgaggtcg ctgaccgcga
     2881 gcgcgtgcat caattcgaca ctgctggccg cgggccggaa gccggccagc gcttcgaatt
     2941 gccctagcgc gaccaataat tcgggcttgt ggctgcggtc gcggtagtta cggatggccg
     3001 agttgacggg gatgcccaac ttgtcctcgc gcgcgaatcc ctcgaccgcc tgctgggcgc
     3061 tcggatgtgc ctgcagcgac agcggttcat cggccgcgag taccttcacc aagaacggca
     3121 agcaatcgcc gaagcggccg cggactgcgg cccccaattg gccttcggga tccttgcgca
     3181 gcgtgtcgag cagggatcgc tcgcccccgt cggtctccag ccacgccgga tcccccgggt
     3241 gcgccccgaa ccacagctca gcctcgggat gcgctgtggg acttggccgt ccggtgaatt
     3301 cggcaatcgc agttcgcgaa ccccaagcat aggtccgcac cgctccgcgt agcaggtgca
     3361 ctcgcttaac ctcgaaccag tcgcaagtag acggccgtca tctccaaccg tacggccagc
     3421 atcgccagtt gttgctccgg ccgcccagtg gcgtccgcca ctacggtctc aggcgcgtcg
     3481 gacatttccg ggacgtcctc ggcattgatc acgtcgacgt cgtcgagtcc gctcacccgc
     3541 gccaccacga ccgggcgctc ggcgtcggtg gccaacacga aggtgcggag ccgcttcggc
     3601 agcggaccgt cgatctgctc gtcatggaat atcgactttt catgatcgac gccggacagt
     3661 ccagcgatac caccgtgcag cgccacgagc gcgtcggcca aaccgaccgc cgccacgctc
     3721 tggtgcgcga tccgcagcat gatcgccgcg ccatggcggg ccagcgtgag cgtggccacg
     3781 gtgtccccgg ccaacacgat gtctcggccc aacatgcggt cggccagcgt cttggcgggg
     3841 ttggtgaaaa gctcacgggc ggcgctgttg cggaacgcct ccgcgtcaag ctcgtcggcc
     3901 agcgcggcga ggtcaacctg cagacccgcg tgtacgacct gcagcacggc gagcccggcc
     3961 gccaggtagc gggacaaacc gaaatcgtcg cggacccgca gccggggcgc cagcgacacg
     4021 gaccgtcctg ccgtcgcatc gcgcagcggg ccctcgtagg gtgcgacgac ggcgatgcgc
     4081 gcgccgcggc gcaccgcggt cgcggcggca tcgacaagcg cgggatcgcc ggggtcgtcg
     4141 ccggcgagga tcaggacgtc gagcgcgccg atccatggcg gcacctcgga cgcgacgaca
     4201 atcggcgagc ccaccgaacc gcccagggtc gcggacagca tcgcccccgc cgtctcggcg
     4261 gttccgcggc cggacaccca gacgactgtg cgcggcggct ggtcggactt caacggctcc
     4321 aggtcacctt catcgagcgc agcggcagta gcgcgcactt gcgcgccagc catcgaggct
     4381 gcgcggagca aaccatcgcg gtcggccgca agcaggcctt ccacgtcgcc gaggtcgacg
     4441 gcggcgtgtg tggcgttcac gtggccgcct tagattctga ggggttttgg gttccgacta
     4501 gtcgggagac ttgcccgacg atctcgtcga ccgcttcggc ggtgcgggcc tcgacgttga
     4561 gccgcagcaa cggctcggtg ttggacatcc gcatgttgaa ccagctgccc tcgccgaggt
     4621 cgacggtgac accgtcgagg tggtcgatgg agtggattcg gctgccgtat gacttcagca
     4681 cggcatcgac acacccctgg gcatcggtga cggtgaagtt gatctcgccc gaggcttcgt
     4741 agcgttggta gtccgccatc agttccgaca aaggccggtc ctgttcgccg agcgcggcga
     4801 gcacatgcag cgcggccagc atgccggagt cggcgcccca gaagtcgcga aagtagtagt
     4861 gcgcggagtg ctcgccaccg aaaatcgctc cggtatcggc catcaacgtc ttgatgtagg
     4921 agtggccgac gcgcgagcgc accggcgtgc cgccccgctc ggcaaccaat tccggaaccg
     4981 cgcgggaggt gatcaggttg tggatcaccg ttgcgccgat ctctctgttc agttcgcggg
     5041 cggccaccaa cgcggtgacc gccgacgggg gcaccggatg ccctttttcg tcgacgacga
     5101 aacaccggtc ggcgtcgccg tcgaacgcca acccgatgtc ggcgcccgtc tcaacgacat
     5161 aggcctgcag gtcgacaagg ttggccggct ccagcgggtt ggcctcgtgg ttggggaagg
     5221 tgccgtcgag ttcgaagtac aacggcagca gcgtgatgga cgggatcacg ccgagcacgg
     5281 cgggtgtggt gtgaccgccc atgccgttgc cggcgtcgac ggcgacgcgc agcggccgta
     5341 attcggacag actgacaagg gaccgcagga actcgccgta gtcggcgagc acgtcgcggt
     5401 cacagatcgt gccgcgcgat ccgtcgaacg cgggcacccc ggcgatgatg tcctcagtta
     5461 tcgcggtcag gccggtctcc ttgccgacag gcttggcgcc ggcgcggcac agcttgatgc
     5521 cgttgtaggc ggccggatta tggctcgcgg tgaacatcgc gcctggacaa tccagcagcc
     5581 ccgacgcgaa atacagttga tcggtcgacg ccagcccgat ccgcaccacg tcaagccctt
     5641 gcgccatgac tccctcggca aacgccgccg ccagcgatgg cgagctcgcc cgcatgtcat
     5701 ggccgatcac cacttgagat gcgccgtcgc gaaccagccg cgcgaacgcg ccgccgacat
     5761 cggcgacgaa accctcgtcg agctcctcac cgaccagtcc gcgaacgtca tacgccttga
     5821 tgacgcgctg aacagccgcc gcgggccgag acatggagct ccttgcgaaa gggaccgatg
     5881 gcgccagcct aaccgtcggc tggcgagcgg atagcggcta ggcaggctct aatcactggg
     5941 gtctggcaat acccgcaggt ggccgcgccg acggccgttc gactcgggcc gcggggcggc
     6001 cggtgccatc agcgccccac cgtgcacacc ggtggccgga tccgaaaagc ccgccgccac
     6061 cccgttgaat gtcggcgaac cgtcgcgtcc ctcccgaacc gcatcggcca acgcgaccag
     6121 atcgtcctca tcggggtgcg acggcagcgg ccccgcgtgg cgaaccagct cccagccgcg
     6181 cggggcggtg atccgacccg cgtgtgccac acacagatcc cacgaatgcg gctcggacac
     6241 cgtggccaag gggccaacga cggccgtcga atcggagtag acgaaggtca gcgtcgcgac
     6301 cgcatagtgg ggacacccgg gccggcagca gcgacgggga acattcacga acgagaggtt
     6361 atcgtgcgga aacgacgtaa gccgccggac acgcgcagcc atgagagccc cgatctccaa
     6421 ccgttacgat cgtggctgcg gacgagagga gcacttgatg agctccgccg agctgaatcg
     6481 acgcggatca cgccgcggcc gggagatgcg cggaccactg cttccgccga cggtgccggg
     6541 ctggcggagc cgggccgagc gattcgacat ggctgtgctg gaagcgtacg aaccgataga
     6601 gcgccggtgg caggaccggg tgtcgggact ggacgtggcg gtcgacgaga taccgcggat
     6661 ctcgcccaag gaccccgacg ctgtgcaatg gccgcccgag gtgctggccg acggcccgat
     6721 cgcactggcc cggttgattc ccgccggggt ggatgtccgc ggcaatgcga cccgggcgcg
     6781 aattgtcttg ttccgcaagc cgattgagcg acgggcgaaa gacaccgtcg aactcgccga
     6841 gctgctgcat gaactcctgg tggctcaggt ggcgacgtat ttgggcgtcg aaccttctgt
     6901 catcgaccca acgatcgacg acgagtgagc gcgctggtta gatgatcccg cgcttgaggc
     6961 ggcggcgctc gcgctcggag agcccgcccc agatcccgaa gcgctcgtca tgggccagcg
     7021 cgtactccag gcacctgtcg cggacctcgc aacccaagca gattcgcttg gcttcgcggg
     7081 tcgagccgcc cttctcgggg aaaaacgcct cggggtcggt ttgagcgcac aatgcgcgct
     7141 cttgccattg atcatcttcg gtagttgccg gagcaacttc atatctctcg ggttgagcgg
     7201 gcaccaaact caattgagga cgtccgggtg tcccaattgg tgtcggtccg gtaatggtgt
     7261 gacgggtgtc gcctactgag ccgagaatcc gattgtcgaa ccggtcctca cgaccgaaat
     7321 cgacctgctc atacgccatg ctccgcctcc tcacctggtc tcgtagatcc ctaatttgag
     7381 ccccactatt gtgatgtgcg gccatcccaa ttcgaacaag tgatcgaatc tcggtctgcg
     7441 acaccggaac cggccggcca accgcgaaat gacactgatg tgattacaca cgtgttagct
     7501 gtcgcggtca agcgctggaa cagaaattca taccattccg tgacaggaat tcggcgcgtc
     7561 gtgacctcgg cgtgtctctc acaccaccgc gccgcaccac ctcagggccc cacggcctag
     7621 tgtcggtcac gtgaaggtca cggttctggt cggcggggtg ggcggcgccc ggttcctgct
     7681 gggcgtgcag cgtctgctgg ggctcggccg ctttagcggc caatccggca cgagcccgtt
     7741 cgtc
//
LOCUS       DASRJA010000596         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2079644,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000596 DASRJA010000000
VERSION     DASRJA010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079644"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFC01_26440"
     CDS             complement(<1..326)
                     /locus_tag="VFC01_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZN83189.1"
                     /translation="MTQFTQAPIFDADQHMYETPESLTKYLPEKYSRAVQFAQIGRQT
                     RIVINNRVNDFIPNPTFERVAAPGAHEKFFAGENTEGLTLREMQGPAIEAPAATRNPA
                     DRVAEL"
     gene            complement(329..1474)
                     /locus_tag="VFC01_26445"
     CDS             complement(329..1474)
                     /locus_tag="VFC01_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZN83190.1"
                     /translation="MNDVAIIGVGLHPFGRFEGKSAMEMGVDAIFAAVADAGVEWKDI
                     QFATGGSWTVANPDAIVGMVGLTGIPFTNVFNACATAASAAKACADGIRLGDYDVGIA
                     IGLDKHPRGAFTEDPALVGMPSWYAENGQYLTTKFFGMKANRYLHDHGISQETLARVA
                     AKNFRNGALNPNAFRRKPIPEDEILNSTMLNYPLTQYMFCAPDEGAAAVVMCRADIAH
                     RYTSKPVYLQAVEVRTRKYGAYEVNTTFAPVDEDVAPTVYAAKSAFEKAGVAPEDVDV
                     IQLQDTDAGAEIIHMAECGFCAAGEQEKLLADGATEIHGAMPVNTDGGLIANGEPIGA
                     SGLRQIHEIVRQLRGEAGDRQVPGEPKVGFTQLYGAPGTAAATILTR"
     gene            complement(1490..1903)
                     /locus_tag="VFC01_26450"
     CDS             complement(1490..1903)
                     /locus_tag="VFC01_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZN83191.1"
                     /translation="MQKALAPDISTWPDESPQLIGSQCGACGATVFPTQPRCPKCSVG
                     EMSEVLLPRRGTLVAWTTQGFPPGAPYKGPTGKDFVPFGVGLVQLGDVIRVEGRLTEN
                     DPAMLEFGMEVELTMVPFATDEDGDELVTFAFKPV"
     gene            complement(1976..2350)
                     /locus_tag="VFC01_26455"
     CDS             complement(1976..2350)
                     /locus_tag="VFC01_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZN83192.1"
                     /translation="MRWIVDGMNVIGSRPDGWWRDRGRAMTALVERLERWAAAEGADV
                     TVVFERPPTPPIESSVITVTHAPEPAANSADDEIVRLVGADTDPSEIRVATSDRELSD
                     RVRAAGASVYPAERLRNLIEPR"
BASE COUNT          375 a          803 c          811 g          459 t
ORIGIN      
        1 tccagttcgg cgacacggtc ggcggggttg cgagtggccg ccggcgcttc gatggccggt
       61 ccctgcatct cgcgcagcgt gagcccctcg gtgttctcgc ctgcgaagaa cttctcgtgc
      121 gcacccggcg ccgcgacgcg ctcgaacgtc gggttcggga tgaaatcgtt gaccctgttg
      181 ttgatgacga tccgggtctg cctaccgatc tgggcgaatt gcacggcacg cgaatacttc
      241 tcgggcagat acttcgtcag cgactcgggt gtctcgtaca tgtgttgatc ggcgtcgaag
      301 atcggcgcct gcgtgaactg cgtcatggtc acctcgtcag gatcgtggcc gcggccgtcc
      361 ccggcgcgcc gtacaactgg gtgaagccga ccttcggttc gccgggcacc tgacgatcgc
      421 ccgcctcgcc gcggagttgc cggacgatct cgtggatctg gcgcagtccc gatgcgccga
      481 tcggctcgcc gttggcgatc agcccgccgt cggtgttgac cggcatcgcg ccgtgaatct
      541 cggtggcgcc gtcggccagc agcttctcct gctcaccggc tgcgcagaaa ccgcactcgg
      601 ccatgtgaat gatctccgcg ccggcgtcgg tgtcctgcag ctggatgacg tcgacgtcct
      661 ccggtgccac accggccttt tcgaacgccg acttggccgc gtacacggtc ggcgccacgt
      721 cctcgtcgac cggggcgaac gtcgtgttca cctcgtatgc gccgtatttc cgggtgcgca
      781 cctcgacggc ctgcaagtac accggcttcg aggtgtagcg gtgtgcgatg tcggcgcggc
      841 acatcaccac ggccgcggcg ccctcgtcgg gtgcacagaa catgtactgg gtgagcgggt
      901 agttcaacat cgtcgaattg aggatctcgt cctcggggat cggcttgcgc cggaaggcgt
      961 tcgggttcaa tgcgccgttg cggaagttct tggccgccac cctggcgagc gtctcttgtg
     1021 agatgccgtg gtcgtggagg tagcgattgg ccttcatgcc gaagaacttg gtggtcagat
     1081 attggccgtt ctcggcgtac cagctcggca tgcccaccag cgcgggatcc tcggtgaagg
     1141 cgccccgtgg gtgcttgtcg aggccgatgg cgatgccgac gtcgtagtcg cccagccgga
     1201 ttccgtcggc gcatgccttg gcggcgctgg ccgccgtcgc gcacgcgttg aacacattgg
     1261 tgaacggaat cccggtcaga ccgaccatgc cgacgattgc gtcgggattc gcgacggtcc
     1321 agctgccgcc ggtggcgaac tggatgtcct tccactccac gccggcgtcg gcgacggccg
     1381 cgaagatcgc gtcgacgccc atctccatcg cggacttgcc ttcgaagcgt ccgaacgggt
     1441 gcaggcccac gccgatgatg gccacgtcat tcattgggcg aatcctctct cagaccggtt
     1501 tgaaggcgaa cgtgacgagc tcgtcgccgt cctcgtcggt ggcgaacggc accatcgtca
     1561 gctcgacctc catgccgaac tcgagcatgg ctgggtcgtt ttcggtcagg cgaccctcga
     1621 cgcggatcac gtcgccgagc tgcacgagcc cgacgccgaa cggaacgaag tccttacccg
     1681 tggggccttt gtagggtgcg ccgggtggga agccctgggt cgtccacgcg acgagggtgc
     1741 cgcggcgggg cagtagcact tcggacatct cgccgacgct gcacttcggg cagcgcggct
     1801 gtgtcggaaa caccgtggcg ccgcatgcgc cgcactggct gccgatcagc tgtgggctct
     1861 catcgggcca ggtcgagatg tcgggcgcga gcgccttctg cattgcgcga tcctccgtcg
     1921 tgggcaccca agtagaagca actgtaaatc ttacagtatc ccctgtgact cgcggctagc
     1981 gcggttcgat gaggttgcgc aggcgttcag ccgggtagac cgacgctccg gctgcccgga
     2041 ctcggtcgga cagctcgcgg tccgaggtgg ccacgcggat ctcggaggga tcggtgtcgg
     2101 cacccaccag ccggacaatc tcatcgtcgg ccgagttcgc ggccggctcc ggcgcatgcg
     2161 tcacagtgat caccgacgat tcgatcggtg gagtcggcgg ccgctcgaac accactgtga
     2221 cgtcagcacc ttcggccgcg gcccatcgct cgagtctttc aaccaacgca gtcatcgcgc
     2281 ggccgcggtc gcgccaccac ccgtcgggcc gggagccgat cacgttcatg ccgtcgacaa
     2341 tccaccgcac ccgcccaggc tatgcccacg ccaagcatgc actgctcgaa gtggcgatgt
     2401 aactgtaaga attacagtat ggtcgtgggg tgatcgagca caagccga
//
LOCUS       DASRJA010000597        11535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2934273,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000597 DASRJA010000000
VERSION     DASRJA010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11535
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2934273"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /gene="pafA"
                     /locus_tag="VFC01_26460"
     CDS             complement(<1..1043)
                     /gene="pafA"
                     /locus_tag="VFC01_26460"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HZN83193.1"
                     /translation="MQRRIMGIETEFGVTCTFHGHRRLSPDEVARYLFRRVVSWGRSS
                     NVFLRNGARLYLDVGSHPEYATAECDNLTQLVTHDRAGERVLEDLLIDAEQRLADEGI
                     GGDIYLFKNNTDSAGNSYGCHENYLIVRAGEFSRISDVLLPFLVTRQLICGAGKVLQT
                     PKAATFCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAEKYRRLHVIVGDSNMCEA
                     TTMLKVGTASLVLEMIESGVPFRDFSLDNPIRAIREVSHDLTGRRPVRLAGGRQASAL
                     DIQREYYARAVEYLQTREPNSQIEQVVDLWGRQLVAVESQDFAKVDTEIDWVIKRKLF
                     QRYQDRYNMELSDP"
     gene            1353..1631
                     /locus_tag="VFC01_26465"
     CDS             1353..1631
                     /locus_tag="VFC01_26465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83194.1"
                     /translation="MGEIVEVDLTQLRTVANRVMDAAEQIAQMRWPALDPDDLQGSAV
                     GNIAAPVLVAAKLTDVVANMRGWAVAAHMSADAFDRAERRNGQRFGAK"
     gene            1628..3235
                     /locus_tag="VFC01_26470"
     CDS             1628..3235
                     /locus_tag="VFC01_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83195.1"
                     /translation="MTRPTVSQAEAWRPDSLRRLADEWDETARRLATHVDTVSDEIRR
                     SRELWTGTTADAAQDNARAITSAGDDAARTLVLASAAARDGADQIAEALKSVRTLVAE
                     ARDGGFDVADDGTVTIRAGPPPLLIALSGGDPAVAHDMLTMRADKLSQQITDALDRLG
                     AADADAARDIEEAFASSALPKAAATVPAGAWPVQASDVVAGWPAMSQDRIGEQIAAMT
                     PAQRQRLIAEFPQQVGNTDGVPWDMRVAANRVNISQAIVDNLGDPAAERRTAFYRTLL
                     GEIDDPTRNGQRIDRQILAFDPARASLVELNGNLSTAKSVAVLVPGLNTTIESSDANM
                     QTSRRFVTATRGNVATITYLGGPFPRGNPVAGVVDAADPRYALAMAPRLVAFSEDVNR
                     TVDATGRPVPVTYVGHSYGGSIVGTAEALGLTSDRTLFVAAAGAGVGVDDPSDWHNRN
                     PDVLRFSMTAPGDFIEAVQGIPGGPHGADTDEMPGVIRLATGYYDDGRLMAGPQAHSD
                     ILNWPSDAWRNILAVITGDSETLHQSS"
     gene            3256..4128
                     /locus_tag="VFC01_26475"
     CDS             3256..4128
                     /locus_tag="VFC01_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83196.1"
                     /translation="MWSRSSPVDGFRLAFDRFGIRGAPAVVLLHGWPGNRHDYRLVVP
                     ILREVADIIVPDLRGFGGSDKHEVAVQHFYSATAQASSIVGLIKELGLSNVVIAGYDV
                     GSRVAQSVARMHPDLVQALVLSPPLPGAGHRVLTAHAQSEFWYQAFHQLPLAAHLIDG
                     NPDAVREYLRHFWTHWSGPNFSVSEDDLDRLVSDYALPGAFTASIAWYRAGAGMVAQS
                     LTELPPDRAIKIHPPTDVLWPQHDPLFPSEWADQLEHYFTDVRLHFGDGAGHFTPLEC
                     PEQFAELILVQQPT"
     gene            complement(4113..5489)
                     /locus_tag="VFC01_26480"
     CDS             complement(4113..5489)
                     /locus_tag="VFC01_26480"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009475690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HZN83197.1"
                     /translation="MTISVFELFKVGIGPSSSHTVGPMRAAANFVADLDAIGVLARVS
                     DVRVELYGSLAATGRGHGTLPAVLLGLEGYRPETIESEEMESARERLRNTGKIMLGGR
                     VPIALSEEDISLYTRTVLDFHPNGMTLTARAADDSTLLRRTYYSVGGGFVVTEGETAT
                     ESDVAHDDTAFSSAAELLALASGRSISDVMLAHECRTRSAEEVRATLLHLRDVMVECE
                     QRGISRTGLLPGALQVRRRAGQWHERLSQEDPHRDPRFAEDWVNLVALAVNEENASGG
                     RIVTAPTNGAAGIIPAVLHYAVHYTEAGVADPDAVAVRFLLTAGAIGSLYKERASISG
                     AEVGCQGEVGSASSMAAAGLAEILGGTPEQVENAAEIAMEHSLGLTCDPIAGLVQIPC
                     IERNAISAGKAINAARMALRGDGEHRVSLDEVIETMRATGRDMSSKYKETSTGGLAVN
                     VSVNYVGC"
     gene            complement(5505..6773)
                     /gene="glyA"
                     /locus_tag="VFC01_26485"
     CDS             complement(5505..6773)
                     /gene="glyA"
                     /locus_tag="VFC01_26485"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZN83198.1"
                     /translation="MSVLDAHLVDFDPEVAELIGKELERQRNGLEMIASENHAPLAVM
                     QAQGSVLTNKYAEGYPGRRYYGGCEHVDVIEQIAIDRIKALFDAEFANVQPHSGAQAN
                     AAVMHALLKPGDTILGLSLDCGGHLTHGMRLNFSGKLYQVAAYGVSPDDYLIDMDQVA
                     EAAREHRPQMIIAGWSAYPRHQDFARFREIADEVGAYLMVDMAHFAGLVAAGLHPSPV
                     PHAHVVTSTTHKTLGGPRGGIILTNDAAIAKKINSAVFPGQQGGPLEHVIAGKAVAFK
                     MAAMPEFVERQMRCIDGAQILAGRLSQPDVRDAGIAVLTGGTDVHLVLVDLRNADIDG
                     RQAEDRLAAIGITVNRNAIPFDPRPPMVTSGLRIGTPALAARGFQAEDFAAVADLIAQ
                     CLIAAPDADTAKLTRAVSELAERHPLYPQL"
     gene            6998..8503
                     /locus_tag="VFC01_26490"
     CDS             6998..8503
                     /locus_tag="VFC01_26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN83199.1"
                     /translation="MLQGREAEPLAEHLNLYIAGEWRPAADGARREIRCPADGQLVAT
                     AAEASSDDTRQAIAAAREAFDAGPWPHTPERERADVLRRTADLIDRDRKDFARAEALD
                     TGKRIVEAEYDMDDVVACLRYYAGVGGTEAGRVVDTGRADAISRLVYEPIGVCGLITP
                     WNYPLLQAIWKVAPALLAGNTFVLKPSELTPSTAVLLMRALEEAGLPAGVANLVLGAG
                     PGAGAPLSEHPDVDMVSFTGGVPSGTGVMAAAAPTVKRVALELGGKNPNVVFADADFE
                     TAVDFALTAVFLHSGQVCSAGARLIVEESLHDRFVDEVVRRAERITLGGPFDPDAETG
                     PLISEAHRDKVEAYVAAGIEEGAVLRCGGRRPDDPRLRDGFFYPPTVLDGCHSGMSVT
                     KDESFGPVLTVETFTDEDDAVRIANDTDFGLAGAVWTQDAGKAQRVAHRLRHGTIWIN
                     DYHPYVPQAEWGGMKRSGNGRELGRAGLDEYREIKHIWQNTNPRPDRWFRG"
     gene            8559..9656
                     /locus_tag="VFC01_26495"
     CDS             8559..9656
                     /locus_tag="VFC01_26495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HZN83200.1"
                     /translation="MATIHTESAPTAVVAPPTPARGDAELSVEGLWKVFGAKASDVAC
                     DETMERLSVQDIKKKTGCAVAVRDVSFDVGCGEVFVVMGLSGSGKSTLVRCLTRLIEP
                     TKGQVVFQGTDVTAAGEKELRDLRRRKVSMVFQHFGLLPHRRVIDNVSYGLEVRGTDK
                     KDRMKRASEVTELVGLAGYENSYPDQLSGGMKQRVGLARALASDPDMLLFDEPFSALD
                     PLIRRDMQNEVIRLHHEMGKTMVFITHDLSEALKLGDRILIMRDGAPVQVGTGDELVG
                     APADDYVREFVSDVPRSRVLTLKWIMREQRPDDALDGPEMAPSTVIRDAVDPVLAADK
                     PIKVVSDGKLIGMVGRSEILAVIGKVDEGGA"
     gene            9656..>11535
                     /locus_tag="VFC01_26500"
     CDS             9656..>11535
                     /locus_tag="VFC01_26500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZN83201.1"
                     /translation="MATLAVGAPSAPQAPEAQPRTSWFSERGPAQFIAAIGAVLGVWI
                     VGFFLLRGRDTLALAPADTKPLHVWLSDLQTSVGEGRNTNPLFTYFFNPIRSVIDAFG
                     TLIADLIAHPVDARPVPYIGWLGVVVLFTFLAWMLGNWKVGLLTLCGLLFMGLQGLWQ
                     DSMETLALTIAAVAISLLIGIPLGIWAGISDGFNRFITPVLDFMQILPSFVYLAPLTL
                     AFLIGPAAAIIATVIYAAPPVIRLTAHGIRSVPSESREAVESLGATRSQELFGALLPM
                     AKRTIVLGINQTIMAALAMVTIAALIAAPGLGQVVIQALSTQDVGTAFNAGLAIVLLA
                     IILDRTTTAASERVEIQRRKSAEPNKYRRPGIAIGAVITAIAIWLSYTYQLVALFPSS
                     LQIGSVHIGLDVGTPIINAANAVTNWAQTSFASVTNGIKDVVTAVALDPLQTLLDSSP
                     WWLTFVAITAIAAIIGGRMAALITVVCLGLIAWPLELWQATMDTLAAVLVATVIVVAL
                     GIVVGVWMGRSPTADKVVRPFLDAAQTMPSFVYLVPFLALFSASRFTGIVAAIVYAAP
                     VSIKIMADGIRGVSPETVEAARSVGSNTWQVITKVQLPMAVRSLTLATNQGLIYVLSM
                     VVV"
BASE COUNT         1982 a         3853 c         3688 g         2012 t
ORIGIN      
        1 gggtcggaca gctccatgtt gtagcggtcc tggtagcgct ggaacagctt gcgcttgatg
       61 acccagtcga tctcagtgtc gaccttcgcg aagtcttggc tctccaccgc gaccagctga
      121 cgtccccaca gatcgaccac ctgctcgatc tgcgaattcg gttcgcgcgt ctgcagatac
      181 tcgaccgcgc gggcgtagta ctcgcgctga atgtcgagcg cgctcgcctg ccgtccgcct
      241 gcgagccgca ccgggcgcct gccggtcaga tcgtggctga cctcgcggat ggcgcggatc
      301 gggttgtcca aggagaagtc gcggaacggc acgccggact cgatcatctc cagcaccagc
      361 gacgcggtgc ccaccttgag catcgtcgtg gcctcgcaca tgttcgagtc accgacgatg
      421 acgtgcagcc gacggtactt ctcggcgtcg gcgtgcggct cgtcacgggt gttgatgatc
      481 ggccgagacc gcgtcgtcgc cgacgagacg ccttcccata tgtgctcggc gcgctgcgac
      541 aggcagaacg tcgccgcctt cggcgtctgc aacaccttgc ccgcaccgca gatcagctgg
      601 cgggtgacca agaacggcaa cagcacgtcc gagatccggg agaactcccc tgcccgcaca
      661 atcagatagt tctcgtggca gccatacgag ttgcccgccg agtcggtgtt gttcttgaac
      721 aggtaaatgt cgccgccgat gccctcgtcg gccagccgct gctcggcgtc gatcagcagg
      781 tcctcaagga cccgctcgcc ggcgcggtcg tgggtgacca gctgggtgag gttgtcgcat
      841 tcggccgtgg cgtactcggg gtggctcccg acatcgagat acaaccgcgc gccgttgcgc
      901 aagaacacgt tcgagctgcg gccccacgac accacccgcc ggaacaaata gcgggcaacc
      961 tcgtcggggc tcagccggcg atggccatgg aacgtgcagg tgacaccgaa ttctgtctcg
     1021 atgcccatga ttcgtcgctg cacgtcatcg agactactgg ttgctggccg agcacggcac
     1081 gcaagccgcg ccgaaccggt ccggttgaca gtggtcggga cgtccgtcaa cgtcagccca
     1141 tgccggaccg ccggcgaggc tgatttggat gccggcggta tgcaccatgc tcacccgcct
     1201 atcttgagcc cgccacaacc ggttgcctga cgaacagacg caaagtcacc caacacgccg
     1261 aagaattagg acactttgcg tctggtcgcg cggtaaagcc aggcctgtgg ataacttcag
     1321 cgccactgcc cgcagatctg tcagcatgtg gcatggggga aatcgttgag gtcgatctga
     1381 cgcaattgcg gacagtcgcc aatcgggtca tggatgccgc cgagcagatc gcgcaaatgc
     1441 gttggcccgc actcgatccc gatgatctgc aggggtctgc cgtcggcaac atcgcagcgc
     1501 ccgtgcttgt cgcggcgaag ctcaccgacg tcgtcgccaa catgcgcggc tgggcggtcg
     1561 ctgcccacat gtcggccgac gcgttcgatc gtgccgaacg ccgcaatggt cagcgattcg
     1621 gcgccaagtg acacggccga ccgtctcgca agccgaagcg tggcggccgg attcgctgcg
     1681 tcggctcgca gatgaatggg atgagaccgc gcgacgtctc gcgacacacg tcgacaccgt
     1741 gtcggacgaa atccgccgca gtcgcgaact ctggaccggc accaccgccg acgccgccca
     1801 ggacaacgcc cgagccatca cctccgccgg cgacgatgca gcgcgcacct tggtcctcgc
     1861 ctccgccgcc gcacgcgatg gcgccgacca gatcgctgag gctctgaagt cggtacggac
     1921 cctggtcgcc gaagcccgcg acggcggatt cgacgtcgcc gacgacggca cggtgacgat
     1981 tcgcgccggt ccgccgccgc tgctcatcgc gctgtccggc ggcgaccccg ccgtcgcgca
     2041 cgacatgctc acgatgcggg ccgacaagct gagccaacag atcaccgacg cactggatcg
     2101 acttggcgcc gccgacgccg acgccgcccg cgacatcgag gaggcgttcg catcgtcagc
     2161 gctccccaag gccgccgcga cggtgccggc tggcgcatgg ccggttcagg catccgacgt
     2221 ggtggcggga tggcccgcga tgagccagga ccgcatcggc gagcagatcg ccgcgatgac
     2281 gccagcgcag cggcagcgcc tcatcgccga attcccgcag caggtcggca acaccgacgg
     2341 cgtgccctgg gacatgcgag tcgccgccaa ccgcgtcaac atctcccaag cgatcgtcga
     2401 caacctcggc gatccggcag ccgagcggcg caccgcgttc taccgcaccc tgctaggtga
     2461 aatcgatgac ccgacccgca acggtcagcg catcgaccgg caaatccttg ctttcgatcc
     2521 cgcccgcgcg tcgctcgtgg aactcaacgg caacctgtcg accgcaaaga gtgtggctgt
     2581 attagtgccg ggcctgaaca ccacgatcga gagttcggac gccaatatgc agacctctcg
     2641 ccggttcgtc acggccaccc gcggcaatgt cgccaccatc acctatctgg gcggcccttt
     2701 cccccgcggt aatccggtag ccggggttgt cgacgccgcc gacccgcggt acgcactcgc
     2761 gatggcgccg cggctggtcg ctttcagcga ggacgtcaac cgcaccgtcg acgcgaccgg
     2821 acggcccgtt cccgtcacct acgtcggcca ttcgtacggc ggatcgatcg tcggcactgc
     2881 cgaggcgctg ggtctgacat cggataggac gctctttgtg gcggcggcgg gcgcaggtgt
     2941 gggcgtggat gaccccagcg actggcacaa ccgcaacccc gatgtgctga gattttcaat
     3001 gaccgcgcca ggggacttca ttgaggcggt acaagggatc cccggcgggc cgcacggcgc
     3061 cgacaccgac gagatgcccg gcgtaatccg tttagccaca gggtattacg acgatggtcg
     3121 gttgatggcc ggcccgcagg cgcacagcga tattctcaac tggccgtctg atgcgtggcg
     3181 caacattctc gcggtcatca ccggggattc ggaaaccttg caccaatcca gctgatatgt
     3241 ggccagaatg ggccaatgtg gtcgcgcagc tcgcccgtcg acgggtttcg gttggccttc
     3301 gaccgcttcg ggatcagggg tgctccagcc gtcgtgctgt tgcacggatg gccgggcaac
     3361 cggcacgact accgcctagt cgtcccgata ctccgcgagg tcgccgacat catcgttccc
     3421 gatctacggg gtttcggcgg ctcggacaag cacgaagtgg cggtgcagca cttctacagc
     3481 gccacggccc aagccagcag catcgtcgga ctgatcaagg aactgggcct gtccaacgtt
     3541 gtcatcgccg gctacgacgt cggcagccgc gtcgcgcaga gtgtcgcccg catgcatccc
     3601 gacctggtgc aggcgctcgt actctctccc ccactacccg gcgcgggcca tcgcgtgctc
     3661 accgcacatg cgcagagcga gttctggtat caggccttcc accagcttcc gctggccgct
     3721 cacctgatcg acggcaatcc tgacgccgtg cgagagtatt tgcgacactt ctggactcac
     3781 tggtccgggc cgaatttcag tgtctccgaa gatgatctgg accgcctggt atccgactac
     3841 gccctcccag gcgcgttcac cgcatcgatc gcgtggtacc gagcaggcgc cggaatggtc
     3901 gcgcagtcgc tcaccgaatt gccccccgac cgcgccatca agattcaccc gccgacggat
     3961 gtgctgtggc cacagcatga tccgttgttc ccgtcggaat gggcagacca gttggagcac
     4021 tacttcaccg atgtgcggct tcactttggt gacggcgcgg gacatttcac accgctcgag
     4081 tgtcctgaac agttcgccga gctcatcctc gttcagcagc cgacgtagtt caccgacacg
     4141 tttacggcaa gcccgccggt ggacgtctcc ttgtacttcg aactcatatc gcgcccggtc
     4201 gcgcgcatgg tctcgatcac ctcgtcaagg ctgacacgat gctcgccgtc tccgcgcagc
     4261 gccatgcgcg cagcattgat ggccttcccg gcagatatcg cgttgcgttc gatgcacggg
     4321 atctgcacga gccccgcgat cgggtcgcac gtcaacccga ggctgtgctc catcgcgatc
     4381 tcggcggcat tctcgacctg ttccggtgtg ccaccgagga tttcggccag tcccgcggcg
     4441 gccatcgacg acgccgagcc gacctcaccc tggcagccga cctcggcgcc ggaaatggaa
     4501 gcccgctcct tgtacagcga cccgatggcg ccggcggtaa gcagaaagcg cacagccaca
     4561 gcatctgggt ccgctacgcc cgcctcggtg tagtgcactg cgtagtgcag caccgcgggg
     4621 atgatccctg ccgcaccgtt ggtcggtgcc gtcacgatgc gcccgcccga ggcgttctcc
     4681 tcgttgacgg ccagcgccac caaattcacc caatcctcgg cgaagcgcgg atcgcggtgc
     4741 gggtcttcct gactgagacg ttcatgccat tgccccgccc gtcgacgcac ctgcagcgca
     4801 ccgggaagca gcccggtgcg gctgatgccg cgctgctcgc attccaccat gacgtcgcga
     4861 aggtgaagca gcgtcgcgcg cacctcctcg gcgctgcgcg tccggcactc gtgggccagc
     4921 atcacgtcgc tgatcgacct gcccgaggcg agcgcgagca gttctgcggc tgaggagaat
     4981 gctgtgtcgt catgggcgac atcggattcg gtagccgtct caccctcggt aacgacgaaa
     5041 ccgcctccga cggaatagta ggttcgccga agcagggtgc tgtcatcggc ggcacgggcg
     5101 gtcagcgtca tgccgttcgg gtggaaatcc agcacggtcc gggtgtacag cgagatgtcc
     5161 tcttcggaca gggcaatagg aacccgcccg ccgagcatga tcttgccggt gttgcgcagc
     5221 cgctcccgcg cagattccat ctcctcggat tcgattgtct ccgggcggta cccctcgaga
     5281 ccgagcagga cggctggcag cgtgccatgc ccccggccgg tcgcggcgag cgacccgtac
     5341 agctcgaccc ggacatcgga cacccgggcg agtaccccga tcgcgtcgag atcggcgacg
     5401 aaattcgcgg ccgcccgcat cgggccgacg gtatgcgaac tcgacggacc gataccgacc
     5461 ttgaagagct cgaagacact gatggtcatt gacagtcccc ctggctatag ctgcgggtag
     5521 agcggatggc gctcggcgag ttcactgacc gcacgcgtca gcttggcggt gtcggcgtcc
     5581 ggcgcggcaa tcaggcactg cgcaatcagg tcggccacgg cggcgaagtc ctcggcctgg
     5641 aaacctcgag ctgccagggc cggcgtgccg atccgcagcc cggaggtcac catcggcggg
     5701 cgcggatcga atggaattgc gttgcggttc accgtgatcc cgatcgccgc cagccgatct
     5761 tcggcctgcc gaccgtcgat gtccgcattc cgcaggtcga cgagcaccaa atggacatcg
     5821 gtgccgccgg tcaaaaccgc gatacccgcg tcacggacat cgggctgcga cagccgccct
     5881 gcgagaatct gcgcgccgtc gatgcatcgc atttgccgct caacgaattc gggcatggcg
     5941 gccatcttga acgccaccgc cttgccggcg atcacatgct ccagcggtcc gccttgctgg
     6001 cctgggaaga cagccgagtt gatcttcttg gcgatggcgg catcgttggt caggatgatt
     6061 ccgccacgtg ggccgccaag ggtcttgtgc gtggtggacg tcaccacatg agcatgtgga
     6121 actggtgacg ggtgcagtcc tgcggcgacg agccccgcga agtgggccat gtcgaccatc
     6181 aggtaggcac cgacctcgtc ggcgatctcc cggaatcggg cgaagtcctg gtggcgcggg
     6241 taggccgacc agcccgcgat gatcatttgg ggccgatgct cgcgggcggc ctcggccact
     6301 tgatccatat cgatgagata gtcgtcgggc gaaaccccat acgcggcaac ttgatatagc
     6361 ttgcccgaga aattcagccg cattccgtgc gtgaggtgac cgccgcagtc aagcgagagc
     6421 ccgaggatgg tgtcccccgg cttgagcagt gcatgcatga ccgcggcatt ggcctgcgca
     6481 ccggaatgcg gctgtacatt ggcgaactcc gcgtcgaaca acgccttgat tcgatcgatc
     6541 gcgatctgct cgatgacgtc gacgtgctcg cagccgccgt aatagcgccg gccagggtaa
     6601 ccctcggcgt acttattagt gagcaccgaa ccctgcgcct gcatgaccgc cagcggtgcg
     6661 tggttctccg acgcgatcat ctccagtccg ttgcgctggc gttcgagttc cttgccgatc
     6721 agctcggcca cctcggggtc gaaatccact agatgtgcgt ccagcacgct catgcagatg
     6781 ctccgttcat tggggtcgac attatgaata ccagattgtc gctaactgat atatcagatt
     6841 gtgacccgca tcatagctgc gtaaagcttt gacggtcaag ggtgcggccc gacgccttgt
     6901 taccagcgag tttcgtttcg ctcctgccct tgacagcgct gtccacgcgg ttcagtctgt
     6961 tgcatgtaga gggttctatt gcgcgatacg caacgatatg ttgcaggggc gagaggccga
     7021 gcctttggcg gaacacctga atctgtacat cgcgggtgaa tggcgccccg ctgctgacgg
     7081 agctcgccgc gagatccgat gtcccgcgga cggtcagctg gtggccaccg cggccgaagc
     7141 atccagcgac gacacccgcc aggcgatcgc cgccgcgcgc gaagcgttcg acgcgggccc
     7201 atggccacac acaccggaac gcgagcgggc cgacgtattg cggcgcaccg ccgatctaat
     7261 cgaccgcgac cgtaaggatt tcgcccgcgc cgaggcgctc gacaccggca agcggatcgt
     7321 cgaggccgaa tacgacatgg acgatgtcgt ggcatgcctg cgctactacg ccggcgtcgg
     7381 cggcaccgag gccggccgcg tggtggatac cggcagggcg gatgcgatca gtcgcttggt
     7441 ctatgagccg attggcgtct gcggactgat cacaccttgg aattaccccc tgctgcaagc
     7501 gatttggaag gtcgccccgg ccctcttggc gggcaacacc ttcgtgctca agccaagtga
     7561 gctgacgccc tcgacggcgg tccttctcat gcgcgcgttg gaggaagcag ggctgcccgc
     7621 cggcgtcgcc aacctggttc tcggcgccgg gcccggcgcc ggcgcgccgc tgtcggagca
     7681 cccggacgtg gatatggtct cgttcaccgg cggcgtcccc tccggcaccg gggtgatggc
     7741 ggcggccgcg ccgaccgtca aacgcgtcgc actggaactc ggcggcaaga atcccaacgt
     7801 cgtcttcgcc gacgccgact tcgaaaccgc ggtggacttc gcgctcaccg cggtgttcct
     7861 gcattcgggt caagtgtgct cggcaggtgc ccgcctgatc gtcgaggagt cactgcacga
     7921 tcggttcgtc gacgaagtgg tccggcgcgc cgagaggatc acgctcggcg gaccattcga
     7981 cccggatgcc gaaacgggcc cgctgatctc cgaggcgcac cgtgacaagg tcgaggccta
     8041 cgtggccgca ggtatagagg aaggcgctgt gctgcgttgc ggcggccgcc gacccgacga
     8101 cccgcgtctg cgcgatggct tcttctatcc gccgaccgtg ctggacggct gccactcggg
     8161 catgtcggtg accaaggacg aatcgttcgg cccggtgctc accgtcgaga ccttcaccga
     8221 cgaagacgac gcggttcgga ttgccaacga caccgatttc gggttggcag gcgcggtctg
     8281 gacccaggac gccggcaagg cacagcgtgt cgcacatcgc ctgcggcacg gaaccatctg
     8341 gatcaacgac taccacccct acgtgccaca agccgaatgg ggcgggatga agcgctccgg
     8401 caacggccgc gaactcggcc gcgccggtct cgacgaatac cgcgagatca agcacatctg
     8461 gcagaacacc aacccccggc cagaccggtg gttccggggc tgacccgggg cccgcagcac
     8521 tccaattgac cgacagccca agaagttagg agatttcggt ggccactatc cacacagaga
     8581 gcgcgcccac ggcggtggtc gctccgccca ccccagcccg cggcgacgcg gaactgagcg
     8641 tcgaaggact gtggaaggtc ttcggcgcca aggcatccga cgtcgcatgc gatgagacga
     8701 tggagaggct cagcgtccag gacatcaaga agaagacggg gtgcgcggtc gccgtgcgcg
     8761 acgtcagctt cgacgtcggc tgcggcgagg tattcgtggt gatgggcctg tccggctccg
     8821 gcaagtccac gctcgtgcga tgtctcacgc ggctgatcga gccgaccaag ggtcaggtgg
     8881 tcttccaggg caccgacgtc acggccgcag gcgagaaaga gctacgggac ttgcgccgtc
     8941 gcaaggtctc gatggtcttc cagcatttcg ggctgctgcc acaccggcgg gtgatcgaca
     9001 acgtttccta cggcctcgag gtccgtggca ccgacaagaa ggacaggatg aagcgcgcca
     9061 gcgaggtcac cgagctggtg gggctggccg gatatgagaa ctcctacccg gatcagctgt
     9121 ccggcggcat gaagcagcgg gtcggccttg ctcgtgccct ggcaagcgat ccggacatgc
     9181 tgctgttcga cgagccgttc tcggcgctgg atccactgat ccgtcgcgac atgcagaacg
     9241 aggtgatccg gctgcaccac gagatgggca agacgatggt gttcatcacc cacgacctgt
     9301 ccgaggcgct caagctcggc gaccggatcc tgatcatgcg cgacggagcg ccggttcagg
     9361 tcggcaccgg cgatgagctc gtcggcgctc ccgccgatga ctatgtgcgc gaattcgtca
     9421 gcgacgtccc gcgttcgcgg gtgctgaccc tgaagtggat catgcgcgag cagcggcccg
     9481 acgatgcgct cgatggccca gaaatggcgc ccagcaccgt gattcgcgat gccgtcgacc
     9541 cagtgcttgc cgccgacaaa cctatcaagg tggtctccga cggcaagctg atcggcatgg
     9601 tcggccgcag cgagatcctc gcggtgatcg gcaaggtcga cgagggcggt gcgtgatggc
     9661 cacactcgcg gtaggggcac cgtcggcacc gcaagcgccg gaggcacagc cgcggacgtc
     9721 gtggttctcc gaacgcggtc cggcgcagtt catcgcggcc attggcgcgg tgttgggtgt
     9781 ctggatcgtg gggttcttct tgctgcgcgg ccgcgatacc ctcgccctcg caccggccga
     9841 caccaaaccg ctgcacgtgt ggctctccga tttgcaaacc tcggtcggcg aggggcgaaa
     9901 caccaacccg ctgttcacct atttcttcaa tccgatccga tcggtgatcg acgcattcgg
     9961 cactttgatc gccgacctga tcgcccatcc ggttgacgca cgacccgtcc cctacatcgg
    10021 ctggctcggc gtcgtcgtgt tgttcacgtt cctggcgtgg atgctcggca actggaaggt
    10081 cggactgctc accctgtgcg gcctgttgtt catgggcctg cagggcctgt ggcaggacag
    10141 catggagacg ctggcgctga cgatcgcagc ggtcgcgata tcgctgctca tcggaatccc
    10201 gttgggcatc tgggccggga tatcggacgg gttcaatcgg ttcatcacgc cggtgctgga
    10261 tttcatgcag atcctgccgt cgttcgtcta cctcgcgccg ctgacgctgg cgttcctgat
    10321 cggccctgcc gcagcgatca tcgcgacggt gatctacgcg gctcccccgg tcatccggct
    10381 cactgcccac ggcatccgat cggtcccctc ggagtctcgg gaagcggttg agtcgctggg
    10441 cgccacgagg tcccaggagt tgttcggggc gctgctgcca atggccaagc gcaccatcgt
    10501 tcttggcatc aaccaaacca tcatggccgc gctggcaatg gtgaccatcg cggcgctgat
    10561 cgccgcaccg ggcctcggcc aagtggtgat ccaggcgttg tcgacccagg acgtcggcac
    10621 cgcattcaac gccggactgg cgatcgtgct gttggcgatc atcctggacc ggaccaccac
    10681 ggccgcgagc gagcgcgtgg aaatccaacg gcgaaagtcg gccgagccca acaagtatcg
    10741 gcgtccgggc atcgcgatcg gtgccgtcat caccgcaatt gcgatttggc tgtcctacac
    10801 ctaccagctc gtcgcgctct tcccgtcatc gctgcagatc gggtcggtgc acatcggact
    10861 cgatgtcggc acgcccatca tcaacgccgc gaacgccgta accaactggg cgcaaaccag
    10921 tttcgcgtcg gtgaccaacg gcatcaagga cgtggtgact gcagtggcgc tcgatccgtt
    10981 gcagacgctg ctggacagct caccgtggtg gctcaccttc gtggcgatca ccgctatcgc
    11041 ggcgatcatc ggaggccgca tggccgcgct catcactgtc gtgtgcctcg gcttgattgc
    11101 gtggccgctt gagctgtggc aggccaccat ggacaccctc gccgcggtgc tggtggccac
    11161 ggtgatcgtc gtcgcgctgg ggattgtggt cggcgtgtgg atgggccgca gcccaacggc
    11221 agacaaggtc gtgcgaccgt tcctggacgc cgcacagacc atgccgtcgt tcgtgtacct
    11281 ggttccgttc ctggcgctgt tctcggcatc gcggttcacc ggcatcgtgg cggcgatcgt
    11341 ctacgccgcg cccgtgtcaa tcaagatcat ggccgacggg atccgcggcg tgtcccccga
    11401 gaccgtcgag gccgcccgct ccgtgggatc gaacacctgg caggtcatca ccaaggtgca
    11461 actgccaatg gccgtccggt ccctgacatt ggccaccaac cagggcctta tatatgtgct
    11521 ctccatggtc gtggt
//
LOCUS       DASRJA010000598        26732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1502329,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000598 DASRJA010000000
VERSION     DASRJA010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 26732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26732
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502329"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFC01_26505"
     CDS             complement(<1..641)
                     /locus_tag="VFC01_26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83202.1"
                     /translation="MTDTRVPARGGRLQMPRSRGAASGFLLILLGAWGALIPFVGPYF
                     DFAFTPDQEWYWTTARGWLEVLPGAATVVGGLLLLGSRNRATAMFGGWLTVLAGAWFV
                     VGRALAGPLGIGDVGAPVAATETKGVWLELTYFYGLGALIVFLGAVALGRLSVRTARD
                     IAYVQRPVAEAAQPQPAPSEEVTEIQPTSTATGERPRRSWRNMFAGGGGHRPA"
     gene            721..1350
                     /locus_tag="VFC01_26510"
     CDS             721..1350
                     /locus_tag="VFC01_26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HZN83203.1"
                     /translation="MRLDDDAVRDTLADAWKRWARRCAELSPQQWSTPTRCRPWDVRG
                     LVAHACPSRSMFDTLTGATSDAHAEVTDAAEMLRRFNAPGGIAHTSADDIADQAATDA
                     TTLTPDDAVMRFTECAEILRATPMSKEVVISYPVVGTTTTLAVVAEIALMEATVHLLD
                     LADAVRGVQPSKPVLAATRDLLIAIADPIAAIEALAGRADPATVLPAIR"
     gene            1474..4986
                     /gene="car"
                     /locus_tag="VFC01_26515"
     CDS             1474..4986
                     /gene="car"
                     /locus_tag="VFC01_26515"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019305838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylic acid reductase"
                     /protein_id="HZN83204.1"
                     /translation="MPTDTHETRLARRISDLYATDQQFASSRPSEAVAQAIESPELRL
                     PQIIQTVIDGYADRPALGQRAVEFVTDPSTSRTSAELLPRFDTITYRELSDRVNAVAA
                     ALAQNPVRPGDRVAILGFTSIDYTTVDMALLRLGAISVPLQTSAPVAQLRPIAAETEP
                     VAILSSVDFLDDAVELMLTGHLPERLVVFDYHAEVDDHRDALAAATTRLAETPVAVET
                     LADVLSRGAALPIPPAFDSDDDATLALLIYTSGSTGTPKGAMYPAKAVMNSWRRSSMA
                     MWGNEGATPSITLNFMPMSHMMGRGILYATLGAGGSAYFVARSDLSTFFEDLALVRPT
                     QLSFVPRIWDMVFQEFQSEVDRRSADGSDRWAVETDVLADLRQNLLGGRFVSAMTGSA
                     PISADMKAFVESLLDIHLTDGYGSTEAGAVFVDGQVQRPPVTDYKLVDVPELGYFHTD
                     RPHPRGELLVKSETMFPGYYKRPEITADVFDVDGYYKTGDIVAEIGPDQLTYLDRRNN
                     VLKLSQGEFVTVAKLEAVFGDSPLVRQIFVYGNSARSYLLAVIVPTEDALASHDVESL
                     KPLIGESLQDVAKAAGLQSYEIPRDFIVETTPFTLENGLLTGIRKLARPKAKEYYGAR
                     LEQLYTDLADSQTNGLRELRLHGADRPVLETISRAAGALLGAAATELQPDAHFTDLGG
                     DSLSALTFANLLHEIFDIDVPVGVIVSPASDLAALAAYIEAERQPGSKRPTFASVHGR
                     DATEVHARDLTLDKFIDAQTLAAAPSLPGPSAEVRTVLLTGATGFLGRYLALEWLERM
                     DLVDGKVIALVRAKDDATARERLDKTFDSGDPELLRHYRDLAAEHLEVIAGDKGEANL
                     GLDQQTWQRLADTVDLIVDPAALVNHVLPYSQLFGPNALGTAELIRIALTTKQKPFVY
                     VSTIGVGAGIELGQFTEDGDVRVISATRKVDDSYANGYSNSKWAGEVLLREAHDLCGL
                     PVSVFRCDMILADTTYAGQLNLPDMFTRLMLSLVATGIAPDSFYELDAAGNRQRAHYD
                     GLPVEFIAEAISTLGERVVDGFETYHVMNPYDDGIGLDEYVDWLIDAGFPVARVGAYD
                     AWLQRFDTTLRALPEKQRQASLLPLIHNYQRPETPIRGSIAPTDRFRTAVQDAKIGPD
                     KDIPHVTREVIVKYITDLQLLGLL"
     gene            complement(5054..5971)
                     /locus_tag="VFC01_26520"
     CDS             complement(5054..5971)
                     /locus_tag="VFC01_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83205.1"
                     /translation="MRRTLRVLIAILAVLAGGCARSAAAPGPSGLFDVGDGRKLFLDC
                     QGSGSPTVFIIPGKGSYTEAWNYVVPPDDPIRSSPYDIITQAKLEPSPDAVQPTVAKT
                     TQVCAYDRPNTRPDGVDRSTDVPQPHGLQQDVDDVVALINASQLPTPLVFVAHSYGGL
                     IADLLARTRPDLVAGLVMVDPVSEFLETLGSPAQNAAWERDSMTPPEPGDETVLVGDA
                     ITLVQNAPPLPRVPAIVLSSDKFASPEVLTPENYTLAQIHQANTLLADALGTTNVVSN
                     GSGHNMMLYQPKFVADNIIGIVDQVRQTG"
     gene            complement(5981..6367)
                     /locus_tag="VFC01_26525"
     CDS             complement(5981..6367)
                     /locus_tag="VFC01_26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011595511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HZN83206.1"
                     /translation="MIYAGLIFAGLAALLHVYIWVMESLTWTSARTRATFGISEEEAV
                     ATKELAFNQGFYNLFLAIVTFTGIVIGGTGRYGIGLALIFAGIGSMLAAAVVLLVSSP
                     DKARAAITQGTLPLIAIVLLAVGVAM"
     gene            complement(6378..7439)
                     /gene="ruvB"
                     /locus_tag="VFC01_26530"
     CDS             complement(6378..7439)
                     /gene="ruvB"
                     /locus_tag="VFC01_26530"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZN83207.1"
                     /translation="MSRFEDGDEVEDREVSAALTVGEGDIDASLRPRSLREFIGQPRV
                     REQLQLVIEGAKNRGSTPDHILLSGPPGLGKTSLAMIIAAELGSSLRVTSGPALERAG
                     DLAAMLSNLVEHDVLFIDEIHRIARPAEEMLYLAMEDFRVDVVVGKGPGATSIPLEVA
                     PFTLVGATTRSGALTGPLRDRFGFTAHMDFYEPAELERVLSRSAGILGIEVGADAGAE
                     IARRSRGTPRIANRLLRRVRDYAEVRADGIITRDIAKSALEVYDVDELGLDRLDRAVL
                     SALTRSFGGGPVGVSTLAVAVGEEATTVEEVCEPFLVRAGMIARTPRGRVATGQAWTH
                     LGMTPPSGVSGLGQAGLFD"
     gene            complement(7436..8026)
                     /gene="ruvA"
                     /locus_tag="VFC01_26535"
     CDS             complement(7436..8026)
                     /gene="ruvA"
                     /locus_tag="VFC01_26535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZN83208.1"
                     /translation="MIASVRGEVLDIALDHVVIEACGVGYKVMATPTTLATLHCGSEA
                     RLITAMIVREDSQTLYGFADADARDLFLTLLGVSGVGPKIALATLAVYDAPSLRQALG
                     DGDVTALTRVPGIGKRGAERMVLELRDKIGPVHAASGAVALNGHSVRGPVVEALVGLG
                     FAVKQAEEATDKVLANEPQATTSSALRSALSMLGKK"
     gene            complement(8023..8577)
                     /gene="ruvC"
                     /locus_tag="VFC01_26540"
     CDS             complement(8023..8577)
                     /gene="ruvC"
                     /locus_tag="VFC01_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZN83209.1"
                     /translation="MRVMGVDPGLTRCGLSVIEGGKGRQVIALDVDVVRTPSDDPLAL
                     RLLAISDTVEHWMDTHRPDVIAIERVFSQQNVPTVMGTAQAGGVIALAAAKRGIDVHF
                     HTPSEVKAAVTGNGNADKAQVTAMVTRILALQTKPTPADAADALALAICHCWRAPMIA
                     RMAAAEALAAEQQRKYKAKLKAAR"
     gene            complement(8648..8953)
                     /locus_tag="VFC01_26545"
     CDS             complement(8648..8953)
                     /locus_tag="VFC01_26545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83210.1"
                     /translation="MAVVLDPHGRAWSVYRDWWPFPGDLFDFTDIFELAIGLLFVVLW
                     PFWLLTKFLGARWVIVVERDGHEVGRELVRGGRKAKGRVNQIAYEIANGHRSGGYQI"
     gene            complement(8944..9540)
                     /locus_tag="VFC01_26550"
     CDS             complement(8944..9540)
                     /locus_tag="VFC01_26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN83211.1"
                     /translation="MARSTRESILTAAAELMRHKGYGAVGMKDIAEASGAPIGSLYHH
                     FRGGKVQIAREALINAGAAYALLIPSIVDSYTDLGDAIDAVFTQAAEDMAGTGFANMC
                     PVASVAAEIADTVEELRETSATVFQGWVDGGSAYFAARGLDASHAREVTFALIGALEG
                     AFVLARTLRNIEPLVAAGHALAPRYRGVELAAKIAAWQ"
     gene            9606..10445
                     /locus_tag="VFC01_26555"
     CDS             9606..10445
                     /locus_tag="VFC01_26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83212.1"
                     /translation="MKSVDVSAGTIEYREEGDPNGPPVVLLHGLLMNDAQWNLALALL
                     PSGFRYLLPILPMGGHRIPMREDADLTLPGMVNIVGDFLDALDLADVTLVVTDWGGAL
                     FLTDIGRDKRLARLVICPSEAFGNFPPGFPGKVAWLASRTTGTVALAMRQLKIGWLRR
                     QWLIFGQMAKNPIPQSIVDTWMAAGLADRRIRRDLVKYCRTKFDKADLIRATNRLADF
                     DGDVLVLWSRNPVMPDDHATRLAELTGATLRYIDDANVLVMLDQPEETAAELGKFLAS
                     TTR"
     gene            complement(10378..11001)
                     /locus_tag="VFC01_26560"
     CDS             complement(10378..11001)
                     /locus_tag="VFC01_26560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83213.1"
                     /translation="MAVAIPRLPDLDSSHEVQAFWRTNQQLMQVVILSVSVGFLFFLM
                     FLGALIELIRTVPDAGAASWTVLGSALMFMTALNVALGLDVAGGLLVDTDSVSTYGLH
                     TAAFLLAAPAAFAGAAFFVAIAVVTWEAKVFPRWSGWIAVVGAGVNAGAVFGIFTLSG
                     PLNSGNGIVGGIAGPLGLYLVWVFAISVWWMRGTFPAPLPSLPAGRA"
     gene            complement(11064..11483)
                     /locus_tag="VFC01_26565"
     CDS             complement(11064..11483)
                     /locus_tag="VFC01_26565"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN83214.1"
                     /translation="MTDDVDVVRRMFTAVEERDFDGLLACYSDDVEINEAAVLPWGGV
                     WRGPEGVAAHAAAFMRTWGSLQGPRESQLDAQFFSDGAGTVCAVFRHRGVDPVSGARF
                     DAPEVGVYRIHDDRVVRSQMFHADSAAVVTFLSNVRQ"
     gene            complement(11476..12084)
                     /locus_tag="VFC01_26570"
     CDS             complement(11476..12084)
                     /locus_tag="VFC01_26570"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN83215.1"
                     /translation="MPRPERHPVDKILDVSRDLVLDHGARAVTVDRIVAASGAPKGSV
                     YHRFSTVSDLLATMWLRGVRRSQARFLEPLKAGGNPMDAALAAGLAICDFATEEPADA
                     RLLAALRREDLVGEVTDPELTSALRQVNRDLHEAVTALARRLYGRATRDAIERTTCAV
                     IDLPQGAIRRHLVQGAPVPRSVRSQLAAAILAALTQEESQHD"
     gene            12162..14129
                     /locus_tag="VFC01_26575"
     CDS             12162..14129
                     /locus_tag="VFC01_26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN83216.1"
                     /translation="MATVRSFCRVCTSVCGILVDIDGDQVVAVHGDRENPFSQGYTCP
                     KGRALPQIHHHPDRLERPQIRVDGTLQDTTWEACLDDLGAKLRDTIETYGPESVGINF
                     GTGVGMDAVGYRVAQQLHAAIGTPAKFSPLTIDGTAKVLMAELMGGTPSLAGRPDYDN
                     ATFAVFIGSNPVVSHGHSVGLPNPRGAIRDLAKHAEVWVVDPRYTETARLATHHLAPR
                     PGTDYAVLAYLVREVLRDGVKAPVQDADALAAAVEPFTLRHASAIADVPEAELERLLS
                     AVRKAGRIAVDTGTGVTMSPSANVTQWLSWALLIITDSMNRPGGIWFHPGFGYQLESF
                     ELPITPPEGRFGPGPRSRPETQAFLREWPCAALPDEINAGNIRAFLNLGGSLLTAFPN
                     AGSLLPALKKLDVLVTTEILPNETTALSTHVLPTKGQLERPDVTLWDFLCPQISAQHT
                     DALVPPVGQRRSMWWVLAEIGRRLGHELADTDASDEVMLAQLSSGGRVPYDELVARGW
                     AETDRELPATWVDKHVERLGGWRLAPRVLVDQLHALRGPAPLVLVPRRQLRRLNAQLD
                     FLGEAVEVVVHPDDAAAAGVSDDQKLIVRSEHGELTGIARVDASVRRGAVSVPHGHAG
                     ANVNLLTSKDDIDLVTGMTRYSGVPVSLHPA"
     gene            complement(14962..15396)
                     /locus_tag="VFC01_26580"
     CDS             complement(14962..15396)
                     /locus_tag="VFC01_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZN83217.1"
                     /translation="MSTAEVARNKETFKRFQDAMNTCDAEFISKSIDELVEPDATIRT
                     PLPGDATGAQVLKQVWSVLLRAFPDLQLDVKDLIGEDDKVVARIVVTGTHRGDYMGVE
                     PTGKSIAYDEIFIFRFANGRVVETWGVVDVYAQMKQLGVIPA"
     gene            complement(15407..16162)
                     /locus_tag="VFC01_26585"
     CDS             complement(15407..16162)
                     /locus_tag="VFC01_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZN83218.1"
                     /translation="MSGHSKWATTKHKKAVIDARRGKNFARLIKNIEVAARVGGGDPA
                     GNPTLYDAIQKAKKNSVPNDNIERARKRGAGEEAGGADYQSITYEGYGPNGVAVLIEC
                     LTDNRNRAAGEVRVAMTRNGGNMADPGSVAYLFSRKGVVTLEKNGLTEDDVLTAVLEA
                     GAEDVNDLDDTFEIICEPGDLVAVRTALQEAGIDYESAEAGFEPSVSVPLDVDGARKI
                     MKLVDALEDLDDVQDVYTNIDIPDDVAAILDEE"
     gene            complement(16228..16662)
                     /locus_tag="VFC01_26590"
     CDS             complement(16228..16662)
                     /locus_tag="VFC01_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83219.1"
                     /translation="MTMIEVEPQPRIDTLVRLATGGEARRTEYTGSVWFDGKPIVAGL
                     TLQRAANAPRGIRMPNFSTSFFLPDSLRYNRTAVGDAALTVSVVLQASGERSAIVHAA
                     ATFTDDPSGAPTWIQAHISGHLGWPAGIAYRIVALTPPDAVR"
     gene            complement(16734..17405)
                     /locus_tag="VFC01_26595"
     CDS             complement(16734..17405)
                     /locus_tag="VFC01_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN83220.1"
                     /translation="MTTQQITALVTGANRGLGRRFAKQLVERGAKVYAAARRPETIDV
                     PGVIPVQLDITDPESVARAAEAAGDVNVLINNAGVSTGASLLTGPLDDVRLEMETHYF
                     GTLAVTRAFAPVIEANGGGSILNVLSVLSWAHVPAFGAYSAAKAAGWAMTDAFRQDLA
                     PRGVHVASLHVGFMDTDMAAVVPADQKVDPSAVAKLALDGLFAGEPEILADDLTRTVK
                     SQLSG"
     gene            complement(17484..18068)
                     /gene="pdxT"
                     /locus_tag="VFC01_26600"
     CDS             complement(17484..18068)
                     /gene="pdxT"
                     /locus_tag="VFC01_26600"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HZN83221.1"
                     /translation="MSTSRVGVLALQGDTREHLAALREAGADATTVRRLNELEAVDAL
                     VIPGGESTTMSHLLREFELLEPLRARLAEGMPAYGSCAGMILLASEILDAGVEGRAAV
                     PLKAIDMTVRRNAFGRQVDSFEGDFPFEGLDDRVHAVFIRAPWVERVGPDVQVLGRAA
                     GHIVAVRQGRMLATAFHPEMTGDRRIHKLFVDML"
     gene            complement(18065..18904)
                     /gene="tesB"
                     /locus_tag="VFC01_26605"
     CDS             complement(18065..18904)
                     /gene="tesB"
                     /locus_tag="VFC01_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HZN83222.1"
                     /translation="MAIEEILDLEQLEVNIYRGRVFSPESGFFQRTFGGHVAGQSLVS
                     AVRTVDDRFQVHSLHGYFLRPGDAKAPTVFIVERLRDGGSFVTRRVNAIQHGETIFSM
                     SASFQTDQSGIEHQDEMPFAPPPDDLPGFISKGGAFDDAGFAQFAEWDVRIVPRDQMK
                     LLPNKASQQQVWFRHRDPLPDDHVLHICALAYMSDLTLLGSAQVTHVEERKHLMVASL
                     DHAMWFMRPFRADEWLLYDQSSPSASGGRSLTQGKIFTQTGEMVAAVMQEGLTRYKRD
                     FTP"
     gene            complement(18915..19832)
                     /gene="pdxS"
                     /locus_tag="VFC01_26610"
     CDS             complement(18915..19832)
                     /gene="pdxS"
                     /locus_tag="VFC01_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HZN83223.1"
                     /translation="METAAGSNGSANSQTGTARVKRGMAEMLKGGVIMDVVTPEQARI
                     AEGAGAVAVMALERVPADIRAQGGVSRMSDPDMIEGIISAVTIPVMAKARIGHFVEAQ
                     ILQSLGVDYIDESEVLTPADYTHHIDKWKFTVPFVCGATNLGEALRRITEGAAMIRSK
                     GEAGTGDVSNATTHMRQIGGELRRLTSLSEDELYVAAKELQAPYELVAEVARAGKLPV
                     TLFTAGGIATPADAAMMMQLGAEGVFVGSGIFKSGDPASRAAAIVKATTFYDDPDVLA
                     KVSRGLGEAMVGINVEDIAAPHRLAERGW"
     gene            complement(19911..20936)
                     /locus_tag="VFC01_26615"
     CDS             complement(19911..20936)
                     /locus_tag="VFC01_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZN83224.1"
                     /translation="MITWVVVIALAVLAVVFLVVAAWAYQTAHRLDRLHVRYDLSWQA
                     LDGALARRAVVGRAVAVEAYAAGPDGKRLATLADAAERAPRTAREAAENELSAALAVV
                     DPASLPVALVAELADAEARVLLARRFHNDAVRDTLALRERPAVRILRLGGTAALPSYF
                     EIAERAETSPTVNRRVSARVVLLDEDGCVLLFCGSDPAIEDGPAPRWWFTVGGAAQQG
                     ETLPAAAARELEEETGLRADPADMVGPVWRRDAVIDFNGSVIRSEEMFFVHRTRRFEP
                     SQAGRTGLERAYIHGHRWCDATMIAELVAKGETVYPLQLGELLEEANALAVARGAVPR
                     RQLQSIR"
     gene            complement(20933..22057)
                     /locus_tag="VFC01_26620"
     CDS             complement(20933..22057)
                     /locus_tag="VFC01_26620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZN83225.1"
                     /translation="MRIGMVCPYSFDVPGGVQSHVLQLAEVMDRRGHEVSVLAPSSPH
                     VKLPDYVVSGGKAVPIPYNGSVARLRFGPATHRMVKRWLADGDFDVLHLHEPNAPSLS
                     MLALNIAEGPIVATFHTSTTKSLTLSVFEPILRPMHEKIVGRIAVSDLARRWQMEALG
                     SDAVEIPNGVDVASFAHAPLLDGYPRPGKSVLFLGRFDEPRKGMPVLLGALPTLVKHF
                     PDIEILIVGRGDEDELREDAGKLAGHLRFLGQVDDAEKASAMRSADVYCAPHLGGESF
                     GIVLVEAMAAGTAVVASDLDAFRRVLVDGEAGRLVRVDDAAGLAAGLVEVLENDVLRE
                     RYIDAATEAVRRYDWSVVARQIMRVYETVAGSGAKVQVAS"
     gene            complement(22057..23004)
                     /locus_tag="VFC01_26625"
     CDS             complement(22057..23004)
                     /locus_tag="VFC01_26625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HZN83226.1"
                     /translation="MSSTPAGQKTDGGSRFPFGGQITDLGYAAGWRVVRAMPEFMARN
                     AFDAGAHYAARNGGPAQLRKNLARVIGTTPARVPDGLIRASVASYARYWREAFRLPSM
                     DLEKQGELLDAHFAGKEHIAAALDTGRGAIMALPHSGNWDMAGVWLVWKHGTFTTVNE
                     RLKPESLYNRFIAYRESLGFEMLPLTGGERPPFEVLADRLRDNRAVCLMADRDLSRSG
                     VQVDFFGEPTRMPAGSAKLAIETGADLIAVHCWYTAHGWEFSASEPLDTSSRDVTTIT
                     QALADRFARNIAAHPADWHMMQPQWIADLSDERRAKLET"
     gene            complement(23001..23663)
                     /locus_tag="VFC01_26630"
     CDS             complement(23001..23663)
                     /locus_tag="VFC01_26630"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZN83227.1"
                     /translation="MSNFYLMTRAAYAKLSKPVAKAALRAGFTPDSITILGTAGTVLS
                     ALTLFPIGQLWWGGVAVWFFVLADMLDGAMARERGGGSRFGAVLDAACDRIGDGAVFC
                     GLLWWAAFGLHSTSLVVATLICLVTSQVISYIKARAEASGLSGEGGIIERPERLIIVL
                     VGAGLSGLFGIPWLLHGAMWLLAVASLVTVGQRMHSVRTSPGAMDPLTNTDAANGEKP
                     AP"
     gene            complement(23660..24217)
                     /locus_tag="VFC01_26635"
     CDS             complement(23660..24217)
                     /locus_tag="VFC01_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HZN83228.1"
                     /translation="MSDPDDRTIVDHGVGEPDHLQRLWTPHRMSYIAEAPMKGGSAAS
                     EQPFTDIPTLSDEEGLVVARGELVYIVLNLYPYNPGHSMVVPYRRVSELEDLTAEESA
                     ELMAFTQKLIRVIKSVSRPHGFNVGLNLGSSAGGSLAEHLHMHVVPRWSGDANFITII
                     GGSKVVPQLLRETRELLASEWAKQP"
     gene            complement(24214..26268)
                     /gene="thrS"
                     /locus_tag="VFC01_26640"
     CDS             complement(24214..26268)
                     /gene="thrS"
                     /locus_tag="VFC01_26640"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZN83229.1"
                     /translation="MSAPASPALAAPIRVAAGTTAGAAVRDADLPGRGAEDAIVVVRD
                     ADGRLRDLSWTPDTDAEVTPVAANTDDGRSVLRHSAAHVLAQAVQDMFPEAKLGIGPP
                     ITDGFYYDFDVPRAFTPEDLEALEKRMRQIVKEGQLFSRRVYESKDQAREELANEPYK
                     LELVDDKSGDPEVMEVGGDELTAYDNLNPRTREREWGDLCRGPHIPTTRYIPAFKLTR
                     SSAAYWRGDQNNASLQRIYGTAWESQDALDKHLQFIEEAQRRDHRKLGAELDLFSFPD
                     EIGSGLAVFHPKGGIIRRELEDYSRRKHSEAGYQFVNSPHITKAELFKISGHLDWYAD
                     GMFPPMHLDAEYNEDGTVRKPGQDYYLKPMNCPMHCLIFRSRGRSYRELPLRLFEFGT
                     VYRYELSGVVHGLTRVRGLTMDDAHIYCTRDQMRDELTSLLGFVLDLLGDYGLTDFYL
                     ELSTKDPDKFVGSDEMWEEATSVLAEVGAASGLDLVPDPGGAAFYGPKISVQVKDALG
                     RSWQMSTIQLDFNFPERFELEYTAADGSRQRPVMIHRALFGSIERFFGILTEHYAGAF
                     PAWLAPVQVVGIPVADDHVPYLNGLATQLKMHGIRAEVDASDDRMAKKIVNHTNQKVP
                     FMLVAGDRDVEANAVSFRFGDRTQLNGIPREEAVEAIVNWIAARENAAPTAELVKVGT
                     PK"
     gene            complement(26329..>26732)
                     /locus_tag="VFC01_26645"
     CDS             complement(26329..>26732)
                     /locus_tag="VFC01_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02611 family protein"
                     /protein_id="HZN83230.1"
                     /translation="KRRWARWRDRLRERPRFELGYRIAIAVVGLAVLGVGILAIPYPG
                     PGWAIVFLGLAILATEFDWARRLLAYTRERYDKVMAWFKKQGLWVQILGAAFTAAVVL
                     GTLWLLGALGWAAELVGIEHRWLKSPIGVGD"
BASE COUNT         4620 a         9120 c         8551 g         4441 t
ORIGIN      
        1 tgcgctggcc tgtggccgcc gccgcccgcg aacatgttgc gccagctgcg acgcggccgc
       61 tcaccggttg cggtgctcgt cggctggatc tcggtgacct cttccgacgg cgccggttgc
      121 ggctgagccg cctcggccac cggccgttgc acataggcaa tgtcgcgcgc cgtccgcacc
      181 gaaagtcgtc ccagcgcaac cgcacccagg aacacgatca gcgcgcccag gccgtagaag
      241 tacgtcagct ccagccacac ccccttggtt tcggtggccg ccaccggcgc acccacgtca
      301 ccaatgccca acggcccggc cagcgcgcgg ccaacgacaa accacgcgcc ggcgagcacc
      361 gtcagccagc cgccgaacat cgcggtcgcg cgattgcgcg agccgagcag cagcaggccg
      421 ccgacaactg tggcggcgcc aggcagaacc tccagccatc ctcgcgcggt ggtccaatac
      481 cactcctggt cgggggtgaa tgcgaagtcg aaatacggtc cgacgaacgg gatgagcgct
      541 ccccacgcgc ccaacaagat cagtaagaat ccactagccg caccacggct gcgcggcatc
      601 tgcagccgtc cgccccgtgc gggaacgcgt gtgtcagtca ttgatgcctc ctcggtcgct
      661 ccgggagtac cccgacctgc gtggccgaaa accgcttgcg gcggtggcta acctggcgcc
      721 atgcgcctcg acgacgatgc cgttcgcgac acgttggccg acgcctggaa gcgatgggcg
      781 cggcgatgcg ccgaactcag cccacagcaa tggtccacgc caactcggtg tcggccctgg
      841 gatgtgcgag gactggtcgc gcatgcatgc cccagccgct ccatgttcga cacgctcacc
      901 ggggcgacga gcgatgcaca cgccgaagtc accgacgccg cggaaatgct gcgccgcttc
      961 aacgcccccg gcggcattgc ccacacgtct gccgacgaca tcgcagacca agccgccact
     1021 gacgcgacga cgctcacgcc cgacgacgct gtgatgcgtt tcactgagtg tgccgaaatc
     1081 ctgcgcgcca caccgatgtc caaagaagta gtgatctcct atcccgtagt tggcactaca
     1141 acgacattgg cggtcgttgc cgaaatcgcg ttgatggagg cgactgtgca cctgctcgat
     1201 ctggccgacg ccgtccgcgg tgttcagccg tcgaaaccag tcctcgcggc gacgcgcgat
     1261 ttgctcatcg ccatcgccga cccgatcgca gcgatcgagg ctcttgctgg ccgggccgat
     1321 ccggcgaccg tgctgccagc tatccggtga cagcggtcac accattcaca gcaaactgac
     1381 agccagcaac ggaataaagt tagctttact aacgtagttt tcattaggct cgacgtggcg
     1441 ccgaaacttc ctgcgatttg ccgaggattc ctgatgccga ctgataccca tgaaacgcgg
     1501 ctcgcacgcc gcatctccga cttgtacgcc acagaccagc agttcgcctc ctcccgaccg
     1561 agtgaggcag tcgcgcaagc gatcgaatca cccgaactgc ggctgccgca gatcatccag
     1621 accgtcatcg acgggtacgc cgaccggccc gccctcgggc aacgcgccgt cgagttcgtc
     1681 accgatccgt cgaccagtcg gacatccgcc gaactgctcc cccgtttcga caccatcacc
     1741 tatcgcgagc tgtcggaccg cgtgaatgcg gtcgccgccg cgctggcgca gaatccggtg
     1801 cggcccggtg accgggtcgc cattctcgga ttcaccagca tcgactacac caccgtcgat
     1861 atggcgctgc tgcgcctcgg cgcgatatcc gtgccgcttc agaccagcgc gccggttgcc
     1921 cagttgcggc ccatcgccgc cgagacggag ccggttgcca tcttgtcgag cgttgacttc
     1981 ctcgacgacg ccgtcgagct catgctgacc ggtcatctgc ccgaacggct ggtggtcttc
     2041 gactaccacg cggaggtgga cgaccaccgc gacgcactcg cggccgcgac cacgcggctc
     2101 gccgaaaccc ctgttgccgt cgagactttg gccgacgtgc tgtcccgcgg cgctgcgctg
     2161 ccaattcctc cggcgttcga ttcggacgac gacgctaccc tggcgctatt gatctacacc
     2221 tcgggcagca ccggcacgcc caagggcgcg atgtaccccg ccaaggcggt catgaactcg
     2281 tggcggcggt ccagcatggc gatgtggggc aacgaaggtg ccactccatc gatcacgttg
     2341 aacttcatgc cgatgagcca catgatgggg cgtggcatcc tgtacgcgac gctcggagcc
     2401 ggcggcagcg cctatttcgt tgccagaagc gatctttcga ccttcttcga agacctcgca
     2461 ctggtgcggc ctacccagtt gagtttcgtg ccgcggatct gggacatggt gtttcaggag
     2521 ttccagagcg aggtcgaccg ccggtccgct gacggcagcg atcgatgggc cgtcgaaacg
     2581 gatgtgctcg ccgacctacg ccagaacctc ctcggtggac gctttgtgtc ggcgatgacg
     2641 ggctccgcgc caatctccgc cgacatgaag gccttcgtcg agtccctgct cgatatccac
     2701 ttgaccgacg gatacggctc caccgaagcc ggcgcggtct tcgtcgacgg ccaggtgcaa
     2761 cgcccgcccg tcaccgacta caagctggtc gacgtgcccg aattgggtta cttccacacc
     2821 gatcggccgc atccgcgggg tgagctgctc gtcaagtccg agaccatgtt ccccggttac
     2881 tacaagcggc cggagatcac cgccgacgtg ttcgatgtcg acggctacta caagaccggc
     2941 gacatcgtgg ccgaaatcgg gccggatcaa ctcacctacc tcgaccgtcg caataatgtc
     3001 ctgaagctct cgcagggtga gttcgtcacc gtcgcgaagt tggaagcggt cttcggtgac
     3061 agccccctgg tgcggcagat cttcgtctac ggcaacagtg ctcgctcgta tctgcttgcg
     3121 gtgatcgtgc cgaccgagga tgcactcgcc agccacgatg tggagtcgct caagccgctg
     3181 atcggcgaat cgctgcagga cgtcgcgaag gctgcgggtc tgcaatcgta cgagatcccg
     3241 cgcgacttca tcgtcgagac aacacctttc acgctcgaga acggtctgct caccggcatc
     3301 cgcaagctcg cgcgcccgaa ggccaaggag tactacggcg cacggctcga acagctctac
     3361 accgacctgg ccgacagcca gaccaacggg ctgcgcgagc tgcgcctgca cggtgccgac
     3421 cgtccggtgc tggagaccat cagccgcgcc gcgggtgcgc tgctgggcgc ggcggcaacc
     3481 gagctgcagc ctgatgcgca cttcaccgat ctgggcggag attcgctgtc cgcgttgaca
     3541 ttcgcgaacc tgctgcatga gatcttcgac atcgacgtgc ccgtcggcgt catcgtcagc
     3601 ccggccagcg acctggccgc cctcgccgcc tacatcgagg ccgagcgcca gcccggcagc
     3661 aagcggccga cgttcgcgtc ggtgcatggt cgtgacgcca ccgaggtaca cgcccgcgat
     3721 ctgaccctgg acaagttcat cgacgcgcag actcttgccg cggctccgtc actgccgggt
     3781 ccgagcgcgg aagtgcgcac ggtgctgctg accggggcga cgggattcct gggccggtac
     3841 ctggccctgg agtggctgga gcggatggac cttgtcgacg gcaaggtgat cgccttggtg
     3901 cgcgccaagg acgacgccac cgcccgcgag cggctggaca agacgtttga cagcggtgac
     3961 cctgaattgc tgcgccatta ccgcgatttg gccgccgagc acctcgaggt catcgcaggc
     4021 gacaagggcg aggcgaacct cggactggat cagcagacct ggcagcggct ggccgacacg
     4081 gtcgatctga tcgtcgaccc cgccgcgttg gtcaaccacg tcctgccgta cagccagctg
     4141 ttcggcccca acgcgctcgg caccgcggag ctgatccgga tcgcgctgac caccaagcag
     4201 aagccgttcg tgtacgtgtc gacgatcggt gtgggcgcgg gtatcgagct cggccagttc
     4261 accgaggacg gtgatgtccg ggtgatcagc gcgacccgca aggtcgacga tagttatgcc
     4321 aatggctact cgaacagcaa gtgggccggc gaggtactgc tgcgcgaggc gcacgacttg
     4381 tgcggcctgc cggtctcagt gttccgctgc gacatgatcc tggccgacac cacctacgcg
     4441 ggccagctca atctgccgga catgttcacc cggctgatgc tgagcctggt cgccacgggc
     4501 atcgcgcccg actcgttcta cgagctcgac gccgcaggca atcggcagcg ggcccactat
     4561 gacgggctgc ctgtcgagtt catcgccgag gcgatctcta cactgggcga gcgggtggtt
     4621 gacgggttcg agacctatca cgtgatgaac ccgtacgacg acggcatcgg gctcgacgag
     4681 tacgtcgact ggctgatcga cgcgggattc ccggtcgcac gcgtcggtgc ctacgacgcg
     4741 tggctccagc ggttcgacac gacgcttcgg gctctgccgg agaagcagag gcaggcctcg
     4801 ctgctcccgc tgatacacaa ctatcagcgg ccggagacac cgattcgcgg gtcgatcgcg
     4861 ccgacggacc ggttccgcac ggccgtgcaa gacgcgaaaa tcggtccgga caaagacatt
     4921 ccgcatgtga cgcgggaggt catcgtcaag tacatcaccg acctgcagct gctcggactg
     4981 ctctaacgcg agagtgcggt ttcgtacgcg acacgcgggt gttagcgtac gaagccgcac
     5041 agtcgcatcc gacttaaccg gtctggcgca cctggtcgac tatcccgatg atgttgtcgg
     5101 ccacgaactt cggctgatac agcatcatgt tgtgaccgct gccgttggac accacgttgg
     5161 tggtgccgag cgcgtccgcc agcaaggtgt tcgcctggtg gatctgcgcc agcgtgtagt
     5221 tctccggcgt cagcacttcg ggcgacgcga acttgtcaga gctgagcacg atggcgggaa
     5281 cgcgtggcag cgggggcgcg ttctggacca gagtgatcgc atcgccgacc agaacagtct
     5341 catccccggg ctcgggcggc gtcatgctgt cacgctccca cgccgcgttc tgcgcgggac
     5401 tacccagcgt ctcgaggaat tccgacacgg gatcgaccat caccaagccc gccaccagat
     5461 cgggacgcgt ccgtgcgagg agatcggcga tcaggccgcc ataggagtgg gcgacgaaca
     5521 ccaaaggcgt gggcagctgc gacgcattga tgagtgcgac cacgtcgtcg acgtcctgct
     5581 gaagaccatg cggctgcggc acatcggttg accggtcgac accgtcgggc cgcgtgttgg
     5641 gccggtcgta tgcgcatacc tgggtcgttt tcgccacggt cggctgcacg gcgtcggggc
     5701 ttggctccag cttggcttgc gtgatgatgt cgtagggcga cgaccggatc gggtcatcgg
     5761 gtggcacaac gtaattccac gcctcggtgt agctaccttt tccagggatg atgaatacgg
     5821 tcggtgagcc agagccttga cagtcaagga acaactttcg gccgtcaccg acgtcgaaca
     5881 gcccggacgg ccctggggcg gccgcgctac gcgcgcatcc acccgccagc acggcgagga
     5941 tcgcaatcag cacacgaagc gttcgcctca cacggcgacg ctacatcgcg acccccaccg
     6001 caaggagcac gattgcgatc aacggcagag ttccttgcgt gatggccgca cgcgccttat
     6061 ccggtgacga caccagcagc accaccgcgg cagccaacat cgacccgatg ccggcgaaga
     6121 tcagggcaag gccgatgccg taccggcccg tgccaccgat cacgatgccg gtgaacgtca
     6181 cgatggcaag gaacaggttg tagaagccct gattgaacgc gagttccttg gtggcaacgg
     6241 cttcttcctc gctgataccg aacgtcgcgc gggtgcgcgc agatgtccag gtcagcgact
     6301 ccatcaccca gatgtagacg tgcagcagcg ccgcaagacc cgcgaaaatc aaaccggcat
     6361 agatcattac ggtcctccta gtcgaacagt ccggcctgac ccagtccgct gaccccactg
     6421 ggcggagtca tgccgagatg cgtccacgcc tggccggtgg cgacgcgccc acgcggggtg
     6481 cgcgcgatca tcccggcgcg taccaggaaa ggctcgcaca cctcctcgac ggtggtggct
     6541 tcctccccca ccgcgaccgc aagcgtcgac accccgacag gtccgccgcc gaaactgcgt
     6601 gtcagagcgg acaacaccgc gcggtccagc cggtccaggc cgagctcgtc gacgtcatag
     6661 acctcaagcg ccgacttcgc gatgtcacgg gtgatgatgc cgtccgcgcg gacctccgca
     6721 tagtcgcgga cccggcgcag cagtcggttc gcgatgcgtg gcgtgccccg cgaacggcgg
     6781 gcgatctcgg cgccggcgtc ggcgcccacc tcgatgccga ggatgcccgc cgagcgggac
     6841 agcacccgct ccaattcggc aggctcgtag aagtccatgt gtgcggtaaa cccgaagcgg
     6901 tcacgcagcg gtccggtcaa cgcaccggat cgggtggtcg ccccgaccaa ggtgaacggc
     6961 gcgacctcga gcggaatcga cgtggcgcca gggcctttcc cgacgacgac gtcgacgcgg
     7021 aagtcctcca tcgccaggta caacatctcc tcggcgggcc gggcgatgcg gtggatctcg
     7081 tcgatgaaca acacgtcgtg ctcgacgagg ttggacaaca tcgcggcgag gtcgcccgcg
     7141 cgctccaacg cgggacccga ggtgactcgc agcgacgacc cgagttcggc ggcgatgatc
     7201 atcgcgagcg acgtcttgcc caacccgggc ggccctgaca gcaggatgtg atccggtgtg
     7261 ctgccgcggt tcttggcgcc ctcgatgacc agttgcaact gctcgcgcac ccgcggctgc
     7321 ccgatgaact cacgcagcga ccgcggtcgc aggctggcgt cgatgtcgcc ctcgccgacc
     7381 gtcagcgcag ccgagacttc gcggtcctcg acctcgtcgc cgtcttcgaa ccggctcact
     7441 ttttacccaa catcgacaac gccgagcgca acgcgctgga cgtggtggcc tgcggctcgt
     7501 tggcaaggac tttgtcggtg gcctcctcgg cctgcttgac cgcaaagcca agtccgacaa
     7561 gcgcttccac gaccgggccg cgcaccgagt ggccattaag agcgacggcg cccgatgcgg
     7621 cgtgcaccgg cccgatcttg tcgcgcaatt ccagcaccat gcgctcggca ccacgcttgc
     7681 cgatgccagg cacccgggta agcgcggtga cgtcgccgtc gccgagggct tggcgcagcg
     7741 agggcgcgtc gtagacggcg agcgtggcca gcgcgatctt cggtcccacc ccggacacgc
     7801 cgagcagcgt caggaacagg tcgcgcgcgt cggcgtcggc gaagccgtag agcgtctgcg
     7861 aatcttcacg cacgatcatc gcggtgatca gcctggcttc gctgccgcag tgcagtgtcg
     7921 cgagggtggt cggcgtggcc atcaccttgt agccgacgcc gcacgcttcg atgacgacgt
     7981 ggtcgagtgc gatgtcgaga acttcgccgc gaacggatgc gatcatcgtg ccgccttcaa
     8041 tttcgccttg tacttgcgtt gttgctcggc ggccagcgcc tcggcggccg ccatccgggc
     8101 gatcatcggg gcccgccagc agtgacagat cgccaatgcc agcgcgtcgg cggcgtcggc
     8161 cggtgtcggt tttgtctgca gcgcaaggat tctggtgacc atcgcggtga cctgcgcctt
     8221 gtctgcgttg ccgttgccgg taacggcggc cttcacctca ctcggcgtgt ggaaatgcac
     8281 gtcgatgccg cgcttggccg ccgcaagcgc gatcacgccg ccggcctgcg ccgtgcccat
     8341 gactgttggg acgttctgct gcgagaagac ccgctcgatc gcgatcacgt cagggcggtg
     8401 ggtgtccatc caatgctcga ccgtgtcgct gatagcgagc agtctgagcg ccagcggatc
     8461 gtcggaggga gtgcggacca cgtcgacgtc caaggcgatg acctgacggc ccttgccgcc
     8521 ctcgatcacg gacaggccac agcgggtcag cccggggtcg actcccatta cccgcacgtc
     8581 agccctcctg tgaacacctg ttcgacacgg tagcgcggtg ggccgacgga tatccgtagg
     8641 gacacgctca gatctggtag ccgccggagc ggtgaccgtt ggctatttcg taggcgatct
     8701 ggttgacccg cccctttgcc ttgcgcccgc cacgcaccaa ctcgcgaccc acttcgtggc
     8761 cgtctcgctc gaccacgatg acccagcgcg cgccgagaaa cttcgtgagc aaccagaacg
     8821 gccacaacac cacgaacagc agcccgattg ccagctcgaa aatgtcggtg aaatcgaaaa
     8881 ggtcgcctgg aaacggccac caatcgcgat ataccgacca ggccctgcca tgcgggtcga
     8941 ggactactgc catgcagcta tcttggctgc cagttcgact ccgcggtatc ggggggccag
     9001 cgcgtgacct gcggcgacca gcggttcgat gttgcgcagc gtgcgggcga ggacgaacgc
     9061 cccctcgagc gcgccgatca atgcgaacgt cacctcgcgg gcatgcgacg cgtcgagccc
     9121 ccgggccgcg aagtacgcgc tgccgccgtc gacccagccc tggaacaccg tcgcggacgt
     9181 ttcgcggagt tcctcgaccg tgtcggcgat ctcggcggcg acgctggcca ccgggcacat
     9241 gttcgcaaaa ccagtacccg ccatgtcctc ggcagcctgt gtgaagacgg cgtcgatcgc
     9301 gtcgccgagg tcggtatagg aatccacgat ggacgggatg agcagtgcgt aggcggcccc
     9361 ggcattgatc agcgcctcgc gcgcgatctg taccttgccg ccgcggaagt ggtgatacag
     9421 cgagccgatc ggcgcgccgg atgcctcggc gatgtccttc atgccgaccg cgccgtagcc
     9481 cttgtggcgc atcagctccg ctgcggccgt caggatcgat tctctggtgg accttgccat
     9541 ctgcgcctcc tgcagacaga ctagagcata cgttctagaa tcaacgttct aacgaagggt
     9601 tacggatgaa atccgtcgac gtcagcgccg gaaccatcga ataccgcgag gaaggcgacc
     9661 cgaacggtcc gcccgtcgtg ctgctccacg gcctgctcat gaacgacgcg cagtggaatc
     9721 tggcgctggc tctgctgccg tccggcttcc gctacctgtt gccgatcctg ccgatgggcg
     9781 ggcaccgcat tccgatgcgc gaggacgcgg atctgacgtt gcccggcatg gtcaacatcg
     9841 tcggagattt cttggacgcg ctcgacctcg ccgacgtcac gctcgtcgtc accgactggg
     9901 gcggggcgct cttcctcacc gacatcggcc gcgataaacg ccttgcgcgc ctggtgatct
     9961 gcccgtcgga agcgttcggc aacttcccgc cgggctttcc aggcaaggtc gcctggctgg
    10021 ccagtcggac cacgggcacg gtcgcgctgg cgatgcggca gctcaagatc ggctggctgc
    10081 ggcggcagtg gctgatcttc ggccagatgg cgaaaaatcc tatcccccaa agcattgtcg
    10141 acacatggat ggcagccggc ctggccgacc gacggatccg gcgcgatctc gtcaagtact
    10201 gccgcacgaa gttcgacaaa gccgacctga tccgcgccac caacaggctg gccgacttcg
    10261 acggcgacgt gctggtgctg tggagccgta atccggtgat gccggacgat cacgcaacgc
    10321 ggctggccga gctgaccggc gcgacgctgc gctatatcga cgacgccaac gtgttggtca
    10381 tgctcgacca gccggaagag acggcagcgg agctgggaaa gttcctcgca tccaccacac
    10441 gctgatggcg aacacccaca ccaggtagag accgagtggc ccggcaatac cgccgacgat
    10501 tccgttgccc gaattcagcg ggccactcag cgtgaaaatg ccgaagaccg cgccggcgtt
    10561 gaccccggcg ccgaccaccg cgatccaccc cgaccacctc gggaaaacct ttgcttccca
    10621 agtgactacg gcgatcgcga cgaagaatgc ggcgcccgcg aaagcggctg gcgccgccaa
    10681 cagaaacgcg gccgtgtgca acccgtaagt gcttaccgaa tcggtgtcga cgagtaggcc
    10741 gccggcaaca tcgaggccca gtgcgacgtt gagcgcggtc atgaacatga gcgcactgcc
    10801 gagaactgtc cagctcgcgg caccggcgtc gggaactgtg cgaatgagtt cgataagggc
    10861 tccaaggaac atgaggaaga acaggaaacc cacactcacc gagaggatca cgacttgcat
    10921 caactgttga ttggtgcgcc aaaacgcctg cacctcatgc gatgagtcga ggtcgggcag
    10981 gcgtgggatg gcgacggcca tcggagccca cgtgacgcag agcaagagac cgctgagagg
    11041 cccccaccga tcccagcgcc tgatcattgc cggacgttgc tgagaaaggt gacgactgcg
    11101 gccgaatcag cgtgaaacat ctgtgatctc accacacgat catcgtggat ccgataaacg
    11161 ccgacctcgg gagcgtcaaa ccgcgctccg ctcaccggat caacgccacg atgccgaaac
    11221 acggcgcaca ccgttcccgc accatcgctg aagaattgcg cgtcgagttg cgactcccgg
    11281 ggcccctgca aggaacccca tgtacgcatg aatgccgccg cgtgggcggc gacaccttcc
    11341 ggtccgcgcc acactccacc ccaaggcaac accgcagcct cgttgatctc cacgtcgtcg
    11401 gagtagcacg caagcagacc gtcgaaatca cgttcttcga ccgcggtgaa catccgtcgc
    11461 accacgtcca catcgtcagt catgttgaga ctcctcctgc gtcaaggccg caaggatcgc
    11521 ggcagccagt tgcgaacgaa cgctgcgcgg cacgggtgct ccttggacaa ggtgtcgacg
    11581 gatcgcaccc tgtggcagat cgatgaccgc gcacgtcgtg cgctcgatcg catcgcgcgt
    11641 cgcgcgcccg tacagacgtc gcgcaagtgc cgtcaccgcc tcgtgcaaat cccggttgac
    11701 ttgtcgaagc gctgatgtca gttctggatc ggtcacctca ccgactagat cctcacgtcg
    11761 cagcgcagcg agcagcctgg cgtctgccgg ctcctcagtc gcgaagtcgc aaatggccaa
    11821 acctgcggcc agcgccgcat ccatcgggtt cccgcccgct ttcaacggct ccaggaagcg
    11881 cgcctgcgac cggcgcacac ctcgcagcca catggtcgcg agcagatcac tcaccgtcga
    11941 aaagcgatgg tagacagagc cttttggcgc gccactggcg gcgacgatcc ggtccaccgt
    12001 gaccgcccgg gcaccgtgat cgagcacaag atcgcgcgac acgtcgagga tcttgtcgac
    12061 tgggtggcgc tccggacggg gcacgtgatc gataatagac gaaatagtct aatatgggca
    12121 gccggtagca cattctctct cggcgagagt acggttctcc catggcaacg gtgcgcagct
    12181 tctgccgggt ctgcacgtcg gtctgcggga tccttgtcga cattgacggt gatcaggtgg
    12241 tcgccgtgca cggcgaccgc gagaatccgt tctcccaggg ctacacctgc ccaaagggcc
    12301 gcgccctgcc gcagatccac caccatccgg accggctgga acgcccgcag atacgtgtcg
    12361 acggcacgct gcaggacacc acctgggagg cctgcctcga tgacctcggc gccaagcttc
    12421 gcgacaccat cgagacgtac ggacccgaat ccgtggggat caacttcggt accggcgtcg
    12481 gtatggacgc ggtcggctat cgcgttgcgc agcaactgca cgccgcgatc ggcacgccgg
    12541 cgaagttcag tccattgacg atcgacggga ccgccaaggt actgatggcc gagctgatgg
    12601 gcggcacgcc gtcactggct gggcgtcccg actacgacaa cgccacgttc gcggtgttca
    12661 tcggcagcaa ccccgttgtc tcgcatgggc atagcgtcgg gctgccgaat ccccgcggcg
    12721 cgatccgcga tctcgcgaag catgccgagg tctgggtcgt cgaccctcgc tataccgaga
    12781 cggcgcggct ggcgacgcat catcttgccc ctcgcccggg caccgactac gcggtgctcg
    12841 cgtacctggt gcgcgaggtg ctgcgcgacg gtgtcaaagc accggtccag gacgctgacg
    12901 cgctggccgc cgccgtcgag ccgttcacgc tgcggcacgc gtccgcgatc gccgacgtgc
    12961 cagaggccga gctcgaacgg ctgctgagcg ccgtccggaa ggccggtcgg atcgctgtcg
    13021 acaccggtac cggtgtcacg atgtcgccga gtgccaatgt gacgcaatgg ctttcgtggg
    13081 cactcctgat cataacggat tcgatgaacc gtcccggcgg aatctggttc catccggggt
    13141 tcggctacca gctcgagagc ttcgaattgc cgatcacgcc gccggagggg cggttcggac
    13201 cgggtccgcg cagccggccg gagacgcagg cgtttctgcg ggagtggccg tgcgccgcgc
    13261 tgcccgatga gatcaacgcg ggcaacatcc gcgcgttcct gaacctcggc ggcagcctgc
    13321 tcaccgcgtt cccgaacgcc gggtcgctgc tgccggcgct gaagaagctc gacgtcctcg
    13381 tgaccacgga gatcctgccg aacgagacaa cggcgttgtc gacgcacgtg cttccgacga
    13441 agggtcagct cgagcggccc gacgtcacgc tgtgggactt cctctgcccg cagatctcgg
    13501 cgcaacacac cgacgccttg gttccgcctg tgggacaacg ccgttcgatg tggtgggtac
    13561 tcgcggagat cggtcggcgc ctcggccacg agctggccga caccgacgcc tccgacgagg
    13621 tgatgctcgc gcagctgagc tcgggcggcc gggttccgta cgacgagctc gtcgcgcgcg
    13681 gctgggccga aaccgatcgc gagctgcctg cgacgtgggt cgacaagcat gttgaacggt
    13741 tgggcggctg gcgcctcgct ccacgcgtac tcgttgacca gttgcacgcc ctgcggggac
    13801 ccgctccgct cgtgctggtg ccgcggcggc agttgcgtcg gctcaatgcg cagcttgatt
    13861 ttctcggtga ggccgtcgag gtcgtcgtac atcccgacga cgcggcggcg gcgggtgtgt
    13921 ccgacgatca gaagctgatc gtgcgtagcg agcatggcga gctgaccggt atcgcgcggg
    13981 tggacgcctc cgttcgccgc ggtgcggtgt cggtcccgca cggccacgcc ggggcaaacg
    14041 tgaatctgct taccagcaag gacgatatcg acctcgtgac ggggatgacg cgttactcgg
    14101 gcgtgccggt cagcctgcat ccggcgtgac attcgccggt ccattggcgc gccgccgcat
    14161 ctgtggctga gcagtcgggc gcccggtgag ggttcgtaat tgagacaggc cgcgaagcga
    14221 caaagcgctc agccatccga gtccggttgc gatcgagatc cgctgcagga tcccgctttt
    14281 gccgcggagg cgggactgac ctctgatcgc ggcgcccacg agaaggaaac tgcccgccat
    14341 caccgtgctc gatcctgccg agtagcaggc ccagcggtag tccctgctcc gcacggcggt
    14401 ggccgcgctg gccaggccag cgatgggtat gcccgcaaag accgggatcc cgcaaaggtt
    14461 gtgcatctgc cccgcacgag ttggcctggt cgggccggca tcggccgggc cccggttcat
    14521 gtcaggaccc tcgctcggaa ggtcaccaac gtaatcggtg acgaacagtc ccgagccaat
    14581 cagcccgatg cccgctcctg cgatgagcgc cgggacggcg cggggtccaa tgcgccgccg
    14641 atcggagcgc gcgagacccg cagccgcaca tgaatagaga actccagcga ggatgaagtt
    14701 ggtgcgctgg tgccaacctt gacgaccgat cgcaagcgaa ctgaccgggt agcgccgcca
    14761 atcgtaccct cgacgcgcgg ctccgatcgc tgtgaaagcc gtgacgaaga gcggcccggc
    14821 aacggcaccg cagaggatct gagcagaagg cttgcgtgat gagcggccgc caactgacac
    14881 ctgcccaggc tacttaaacg ttgggccgtc tggggcctcg gcacgccacc gccaagttcg
    14941 catcgcggtg gcgatgacgt tctaggcggg aatcactccc agctgtttca tctgcgcata
    15001 gacgtcaacg acgccccaag tctcaacgac tcggccgttg gcgaagcgga agatgaagat
    15061 ctcgtcgtac gcaatggatt tgcctgtcgg ctccacgccc atgtagtcac cccggtgcgt
    15121 ccccgtgacc acgatcctcg cgacgacctt gtcgtcctcc ccgatcaagt ccttgacgtc
    15181 gagctgcaag tcggggaagg cccgcaggag caccgaccac acctgcttga ggacctgcgc
    15241 gccggtcgcg tcacccggca gcggcgtgcg gatcgtggcg tcgggctcga cgagttcgtc
    15301 gatcgacttc gagatgaact ccgcgtcaca ggtgttcatg gcgtcttgga agcgcttgaa
    15361 ggtctccttg ttgcgtgcta cctcggctgt cgacatgcgg cctgggctac tcctcgtcga
    15421 ggatggcggc gacgtcgtcg ggaatgtcga tgttggtgta gacgtcctgc acgtcgtcga
    15481 gatcctcgag cgcgtcgacc agtttcatga tcttgcgcgc gccgtcgacg tcgagtggca
    15541 cgctgaccga tggctcgaag ccggcctcgg cggactcata gtcgatgccc gcctcctgca
    15601 gcgcggtgcg cactgcgacc agatcgcctg gctcgcagat gatttcgaaa gtgtcatcga
    15661 gatcgttgac gtcttccgca cccgcttcga gcacggcggt cagcacgtcg tcctcggtca
    15721 gcccgttctt ctccagcgtg acgacaccct tgcgggagaa caggtaggcc accgacccgg
    15781 ggtcggccat gtttccgccg ttgcgggtca tcgcgacgcg cacctcgccc gcggcgcggt
    15841 tgcggttgtc ggtcaggcac tcgatcagca ccgcgacacc gttgggtccg tagccctcgt
    15901 aggtgatgct ctggtagtcg gcgccgccgg cctcctcgcc tgcgccacgc ttgcgggccc
    15961 gctcgatgtt gtcgttgggc accgagttct tcttggcctt ctgaatggcg tcatacaacg
    16021 tcgggttgcc ggcgggatca ccgccgccga cgcgcgcggc cacctcgatg ttcttgatca
    16081 accgcgcgaa gttcttgccg cgacgggcgt cgatcacagc cttcttgtgc ttggtggtgg
    16141 cccacttgga atggccgctc atgcaggtac tgctctcttt cttacgttgc aaagtccgat
    16201 caacgagtct acgtggacgt ttatgcctta ccgaaccgca tcgggtggcg tcagtgcgac
    16261 gatccggtac gcgatacctg ctggccagcc cagatggccg ctaatgtgcg cctgaatcca
    16321 ggtcggggca cccgacgggt cgtcggtgaa cgtcgcggcg gcgtggacga tggcgcttcg
    16381 ctcgccggag gcctgcaaga ccaccgagac ggtcagcgcc gcgtcgccaa cggcggtccg
    16441 gttgtagcga aggctgtcgg gcaggaagaa cgacgtcgag aagttcggca tccggatgcc
    16501 ccttggcgcg ttcgcagcgc gctgaagcgt cagcccggcg acgatcggct tgccgtcgaa
    16561 ccacacggag ccggtgtact cggtccgtcg ggcctcgccg cctgttgcca gccgtaccaa
    16621 cgtgtcgatg cgcggttgcg gctccacttc gatcatcgtc atgggcatac tctgcgcgac
    16681 ttgtgcacga tttcacgcgc ttctcagcgc cgaaaaacgt gcactagtcg ccgctagccg
    16741 gaaagttgcg acttcaccgt gcgcgttaga tcgtccgcga gaatctccgg ctcacccgca
    16801 aacaggccgt ccagcgcgag tttcgcgact gccgagggat cgaccttctg atccgccgga
    16861 acgaccgcgg ccatgtcagt gtccatgaag ccgacatgca gggaagcgac atgaacgccg
    16921 cgcggggcga ggtcctgtcg gaacgcatcg gtcatcgccc acccggccgc cttcgcggct
    16981 gaataggcgc cgaaggcagg cacatgcgcc cacgacaaca ccgagagcac gttgaggatc
    17041 gacccgccgc cgttggcttc gatgaccggc gcgaacgcgc gggtgacagc gagcgtgccg
    17101 aagtaatgcg tctccatctc caggcgcaca tcgtcgagcg gcccggtgag cagtgacgcg
    17161 cccgtcgaga cgcccgcgtt gttgatcagc acgttgacgt cgccagccgc ttcggcggcg
    17221 cgggcgacgg actccggatc ggtgatgtcg agctgcacgg ggatgacgcc cggcacgtcg
    17281 atagtctcgg gccgccgcgc ggcggcatat accttcgcgc cgcgctcgac gagctgttta
    17341 gcgaagcgcc tgcccaatcc gcgattggct ccggtcacga gtgcggtgat ctgttgtgtc
    17401 gtcatggttc aaacaaaccc aatttcgtac cgatcattcc ataactggca aagtgtggca
    17461 cccgtcacaa ctcggtagag catctagagc atgtcgacga agagcttgtg gatgcgacgg
    17521 tcgccggtca tctccggatg aaacgccgtc gccagcatgc ggccctgccg caccgcgacg
    17581 atgtgcccgg cggcccggcc cagcacctgc acgtcgggtc ccacgcgttc cacccaaggc
    17641 gcacggatga acaccgcatg cacgcgatcg tcgagcccct cgaacggaaa gtcgccctca
    17701 aacgaatcga cttggcggcc aaaggcgttg cggcgcaccg tcatatcgat ggccttgagc
    17761 gggaccgcgg cgcggccctc gacacccgcg tccagaatct cggacgccag caggatcatc
    17821 ccggcacacg agccgtaggc cggcatgcct tcggccagcc gtgccctcag cggctcgagc
    17881 agttcgaact cgcgcagcaa gtggctcatc gtggtggact cgccgccggg tatcaccagc
    17941 gcgtcgacgg cctcgagttc gttgagtcgg cgaacggtcg tcgcgtcggc gccagcctca
    18001 cgcagcgccg ccagatgttc ccgggtgtcg ccctgcagcg cgagcacgcc gacgcgcgac
    18061 gtgctcatgg cgtgaagtcg cgcttgtagc gggtcaatcc ttcttgcatc accgccgcca
    18121 ccatctcgcc ggtctgcgtg aagatcttgc cctgggtcag cgagcgacca cccgacgccg
    18181 acggcgacga ctggtcgtag agcagccatt cgtcggcgcg gaacggccgc atgaaccaca
    18241 tcgcgtggtc cagcgacgcc accatcaggt gcttgcgttc ctcgacgtgg gtgacctggg
    18301 ccgagccgag cagcgtgagg tcgctcatgt aggccagcgc gcagatgtgc agcacgtgat
    18361 cgtccggcag cggatcgcgg tgccggaacc acacctgctg ctgtgacgcc ttgttgggca
    18421 gcagtttcat ctgatcgcgc gggacgatcc tgacgtccca ctccgcgaac tgcgcgaaac
    18481 cggcgtcgtc gaacgccccg cccttcgaga tgaagccggg caggtcgtca ggcggcggcg
    18541 cgaagggcat ctcgtcctga tgctcaatac cgctctgatc ggtctgaaac gacgccgaca
    18601 tcgagaagat cgtctcgccg tgctggatcg cgtttacccg acgagtgaca aacgagccgc
    18661 cgtcacgcaa gcgctcgacg ataaacaccg tgggggcctt ggcatctccc ggccgcagga
    18721 agtatccgtg cagcgagtgc acctgaaagc gatcgtcgac cgtgcgtacc gcggacacca
    18781 gcgactgacc tgctacatgg ccgccgaagg tgcgctgaaa gaagccggat tcggggctga
    18841 acacacgccc gcggtagatg ttgacctcga gttgttcgag atcaaggatc tcttcgatcg
    18901 ccatgcagtg ttgtctacca gccgcgttct gcgagccgat gcggggccgc gatgtcctcc
    18961 acgttgatgc cgaccatggc ctcccccagc ccgcgtgaca ccttggccaa cacgtcgggg
    19021 tcgtcgtaga aagtggttgc cttcacgatc gcagccgccc gcgacgccgg gtcaccggac
    19081 ttgaagatgc ccgagccgac gaacacgccc tcggcgccca gctgcatcat catcgccgca
    19141 tcggccggcg tcgcgatacc gcccgcggtg aacagcgtca ccggcagctt gcccgcccgc
    19201 gccacctccg cgacaagttc gtagggcgcc tgcaattcct ttgccgcgac gtacaactcg
    19261 tcttcggaga gcgacgtcag cctgcggagc tcgccgccga tctgacgcat gtgcgtggtc
    19321 gcattcgaca cgtcgcccgt gccggcctca cccttggacc ggatcatcgc cgcgccctcg
    19381 gtgatgcggc gcagcgcctc gcccaggttg gtcgcaccgc acacgaacgg cacggtgaac
    19441 ttccacttgt cgatgtggtg ggtgtagtcc gcaggcgtca gcacctcgga ctcgtcgatg
    19501 tagtccacac cgaggctctg cagaatctgc gcctcgacga aatgcccgat gcgggccttg
    19561 gccatcaccg ggatggtgac cgcggagatg atgccctcga tcatgtcggg gtcgctcatc
    19621 cgcgacaccc cgccctgcgc gcggatgtcg gcgggtacgc gctccaacgc catcacggcc
    19681 acggcgcccg cgccctcagc gatgcgggcc tgctcgggag tgacgacgtc catgatcacg
    19741 ccgcccttga gcatctcggc catgccgcgc ttgacccgcg ccgtcccggt ctggctgttg
    19801 gccgacccgt tcgatccagc tgcggtttcc actgctatct cctccaaatc gctactgatc
    19861 cagtctaaag gtggcggcaa atgtgttaaa tccgcagcct cggtgccacc tcagcggatc
    19921 gattgcagct gccgccgcgg cacggcgccc cgtgcgacgg ccagcgcatt cgcctcctct
    19981 aaaagctcac ccagttgcag cggatacacg gtctcacctt tggcgaccag ctcggcgatc
    20041 attgtcgcat cgcaccaacg atggccgtgg atgtaggccc gctccaaccc ggtgcggccg
    20101 gcctgcgacg gctcgaaccg ccgggtgcgg tgcacgaaga acatctcctc gctgcggatg
    20161 accgagccgt tgaagtcgat gaccgcgtcg cgtcgccaca ccggtccgac catgtcggcc
    20221 ggatcagcgc gcagcccggt ctcctcctcc agttcgcgcg ccgccgccgc aggcagcgtc
    20281 tcgccctgct gggccgcccc gccgacggtg aaccaccacc gcggcgccgg accatcctcg
    20341 atcgccgggt cggacccgca gaacagcaag acgcagccgt cctcgtcgag cagcacgacg
    20401 cgggcggaca cccgccggtt gaccgtcggc gacgtctctg cgcgttctgc gatctcgaaa
    20461 tagcttggta gcgcggcggt tccgccgaga cgcaggatgc gcaccgcggg ccgttcgcgc
    20521 agcgcgaggg tgtcgcggac agcgtcgttg tggaagcgac gggccagcag cacccgcgcc
    20581 tcggcatcgg cgagctcggc gacgagcgcc accggcagcg acgccgggtc gactacagcc
    20641 agcgctgcgg acaactcgtt ttcggcggcc tcgcgggcgg ttcgcggtgc ccgctcggcg
    20701 gcgtcggcca acgtcgccag tcgcttgccg tccggccctg cggcgtacgc ctcgacggcg
    20761 actgcgcgcc ccaccaccgc acgcctggcc agcgccccat cgagggcctg ccaggacagg
    20821 tcgtagcgca cgtgcagccg gtccagccga tgcgcggtct ggtacgccca ggcggccacc
    20881 acgaggaaca cgacagccag caccgcgagc gcgatgacca cgacccacgt gatcaactgg
    20941 ccacctgcac cttcgccccc gacccggcga cggtctcgta gacccgcatg atctggcggg
    21001 ccacgacgga ccagtcgtag cggcgcaccg cttcggtcgc ggcgtcgatg tagcgctccc
    21061 gcaagacgtc gttctccagc acctcgacga gccccgccgc caacccagcg gcgtcgtcga
    21121 cccgaaccag ccgcccggcc tcgccgtcga cgagaaccct gcggaacgcg tcgaggtcgc
    21181 tggccaccac cgctgtgccc gccgccatcg cctcgacgag cacgatgccg aagctctccc
    21241 cgccgaggtg gggcgcgcag tacacgtcgg cgctgcgcat cgccgacgcc ttctccgcat
    21301 catcgacctg cccgaggaaa cggagatgcc cggcgagctt tccggcgtcc tcgcgcagtt
    21361 cgtcctcgtc cccgcggccg acgatcagga tctcgatgtc cgggaagtgc ttcaccagcg
    21421 tcggtagggc gcccagcagc acgggcatgc ccttgcgcgg ctcgtcgaag cgaccgagga
    21481 acagcaccga cttgcccggc cgcgggtagc cgtcgagcag cggtgcgtgc gcgaacgacg
    21541 cgacatccac gccgttagga atttcgacgg catcgctgcc cagcgcctcc atctgccagc
    21601 gccgcgccag gtcggaaacc gcgatgcggc cgacgatctt ctcgtgcatc ggccgcagga
    21661 tcggctcaaa aacgctgagg gtcaacgact ttgtcgtcga cgtgtggaac gtcgcaacga
    21721 tcgggccctc ggcgatgttc agcgccagca tcgacaggct cggcgcgttc ggctcgtgca
    21781 gatgcagcac gtcgaagtca ccgtcggcaa gccagcgctt gaccatgcga tgggtggcgg
    21841 gcccgaaccg cagccgggcc accgacccgt tgtacggaat cggcaccgcc ttcccgccgg
    21901 acaccacgta gtcggggagc ttcacgtgcg gcgacgacgg cgccagcacg ctgacctcat
    21961 ggccgcgcct gtccatcacc tcggcgagct gcagcacgtg tgactgcacg ccgccgggga
    22021 cgtcgaacga atacgggcag accatgccga tgcgcatcag gtctccagtt tcgcccggcg
    22081 ctcgtccgag agatcggcta tccactgcgg ctgcatcatg tgccagtcgg ccgggtgggc
    22141 ggcgatgttg cgggcgaacc tgtcggccag tgcctgggtg atggtggtga cgtcgcggga
    22201 cgacgtgtcc agcggctcgc tcgcgctgaa ctcccaaccg tgcgcggtgt accagcagtg
    22261 caccgcgatg aggtcggcgc ccgtctcgat cgccagcttc gccgaacctg cgggcatacg
    22321 ggtgggttcc ccgaagaagt cgacctgaac accactgcga ctcaggtccc ggtcggccat
    22381 caggcagacc gcccggttgt cacgtagccg atccgccagc acctcgaagg gcggccgctc
    22441 gccaccggtc agtggcagca tctcgaatcc gaggctttcg cggtaggcga tgaaccggtt
    22501 gtacagggac tcgggcttga gccgctcgtt cacggtggtg aaagtcccgt gtttccagac
    22561 gagccatact ccggccatgt cccagttccc gctgtgcggc agtgccatga tcgcgccgcg
    22621 gccggtatcc agcgcggcgg cgatgtgctc cttgcccgca aagtgcgcgt caagcagctc
    22681 gccttgcttc tccaggtcca tcgagggcaa tcgaaatgcc tcccgccaat atcgggcgta
    22741 tgaggccacc gaagcacgaa tcagcccgtc cggcaccctg gcaggcgtcg tgccgatgac
    22801 gcgggccaga ttcttgcgca gctgagcggg tccgccgttg cgcgccgcgt agtgcgctcc
    22861 ggcgtcaaaa gcgttgcgcg ccatgaattc cggcatcgcg cgcacgacgc gccaaccggc
    22921 ggcatagccc aagtccgtga tctggccccc aaacggaaac cggctgccgc catctgtctt
    22981 ctgtcccgcc ggggtgctgc tcatggcgcg ggcttctcgc cattggccgc atcggtgttc
    23041 gtcagcgggt ccatcgcgcc cggcgacgtc cgcacgctgt gcatccgctg ccccactgtg
    23101 accagactgg ccaccgcgag cagccacatc gccccgtgca gcagccacgg aataccgaac
    23161 aaaccggaga gtcccgcgcc gaccagcacg atgatgagcc gctcgggccg ctcgatgatg
    23221 ccgccctcac ctgacaggcc gctggcctcg gcgcgcgcct tgatgtagga gatgacctgc
    23281 gaggtgacca ggcagatcag cgttgccacc accagcgacg tgctgtgcag accgaacgca
    23341 gcccaccaca gcaggccgca gaacaccgcg ccgtcgccga tgcggtcgca ggcggcgtcc
    23401 agcaccgcgc cgaacctgct gccgccgcca cgctcgcgcg ccatcgcacc gtcgagcatg
    23461 tcggcaagta cgaagaacca caccgcgacc ccgccccacc acagctgacc gatcgggaac
    23521 aacgtcaacg cgctcaggac ggtcccggcc gtgccgagaa tggtgatgct gtcgggggtg
    23581 aagccggccc gcagcgccgc cttcgcgacc ggcttcgaca gctttgcgta cgccgcgcgg
    23641 gtcatcagat agaaattgct cacggctgtt tcgcccactc agaggccaaa agctcgcgtg
    23701 tctcacgcag caactgcggg acaaccttgg acccgccgat gatcgtgatg aagttggcgt
    23761 cgccgctcca gcgtggcacc acgtgcatgt gcagatgctc ggccagcgac ccgcccgccg
    23821 aactgccgag gttcagcccg acgttgaatc cgtgcggtcg cgacaccgac ttgatgacgc
    23881 gaatcagctt ctgggtgaac gccatcagtt ccgcgctctc ctcggcggtg aggtcttcga
    23941 gctcggatac ccgccggtat ggcaccacca tcgagtgccc tgggttgtac gggtacaggt
    24001 tcagcacgat gtagacgagt tcgccgcgtg ccacgacgag gccttcctcg tccgagagtg
    24061 tcgggatgtc ggtgaacggc tgctccgatg ccgcggaccc gcccttcatc ggcgcctcgg
    24121 cgatgtagga catccggtgc ggcgtccaca ggcgttgcaa gtggtcgggc tcacctacgc
    24181 cgtggtcgac gatcgtgcgg tcgtcgggat cgctcacttg ggcgtgccca ccttgaccag
    24241 ttcggctgtc ggagcggcgt tttcgcgggc ggcgatccag ttcacaatgg cctcgacggc
    24301 ctcctcgcgc gggatgccgt tgagctgagt gcggtcgccg aagcggaagc tgaccgcgtt
    24361 ggcctcgacg tcacggtcgc cggcaaccaa catgaacggg accttctgat tggtgtggtt
    24421 gacgatcttc ttggccatcc ggtcgtcgct ggcatccacc tcggcccgga tgccgtgcat
    24481 cttcagttgc gtggcaaggc cgttgaggta cggaacgtgg tcatcggcaa cagggattcc
    24541 gacgacctgc accggcgcaa gccatgcggg aaacgcaccg gcgtagtgct cggtgagaat
    24601 gccgaagaac cgctcgatcg aaccgaacag cgcgcggtga atcatcaccg gccgctgcct
    24661 gctgccgtcg gctgcggtgt attccagctc gaaacgctcc gggaagttga agtccagttg
    24721 gatcgtcgac atctgccaac tgcggcccag cgcgtccttc acctgcaccg agatcttggg
    24781 gccgtagaac gccgcgccgc ccgggtccgg caccaggtcc agcccggacg ccgcgccgac
    24841 ctccgcgagc acgctggtcg cttcctccca catctcgtcg gagccaacga acttgtccgg
    24901 atccttggtc gacagctcga gatagaaatc ggtgaggccg taatcaccga gcagatcgag
    24961 cacgaacccc aacagcgacg tcagctcgtc gcgcatctgg tcacgggtgc aatagatgtg
    25021 cgcatcgtcc atggtcagac cgcgcacgcg ggtcagcccg tgcaccacgc cggaaagctc
    25081 gtagcggtag acggtgccaa actcgaagag ccgcaacggg agttcgcgat atgaccgtcc
    25141 gcgcgaccgg aagatcaggc agtgcatcgg acagttcatc ggcttgaggt aatagtcctg
    25201 gcccggcttg cgcaccgtgc cgtcctcgtt gtactcggcg tcgaggtgca tcggggggaa
    25261 catgccgtcg gcgtaccagt cgaggtgacc cgagatcttg aacagctccg ccttggtgat
    25321 atgcgggctg ttgacgaact ggtatccggc ctcgctgtgc ttgcgccgcg agtagtcctc
    25381 cagctcgcgc cggatgatgc cgcccttggg gtggaaaacg gctaggcccg aaccgatttc
    25441 gtcggggaag ctgaacaggt cgagttccgc gcccagcttg cggtgatcgc ggcgctgcgc
    25501 ctcctcgatg aactgcaggt gcttgtcgag tgcgtcctgc gactcccagg cggtgccata
    25561 gatgcgttgc aggctggcgt tattctgatc gccccgccag taagcggccg agctgcgggt
    25621 cagcttgaat gccggaatgt agcgcgtcgt cgggatgtgc gggccacggc acagatcacc
    25681 ccattcccgt tcgcgggtac ggggattgag gttgtcgtag gcggtgagct cgtcgccgcc
    25741 gacctccatc acctcggggt caccggactt gtcgtcgacg agctcgagct tgtacggctc
    25801 gttcgcgagt tcttcgcggg cctggtcctt ggactcgtag acgcgccgcg agaacagctg
    25861 cccttccttg acgatctggc gcatccgctt ttccagcgct tccagatcct ccggggtgaa
    25921 cgcgcgcggc acgtcgaagt cgtagtagaa gccgtcggtg atgggcggcc cgatgccgag
    25981 cttggcctcg gggaacatgt cctgcaccgc ctgcgcgagc acgtgcgcgg ccgagtgcct
    26041 gagcacgctg cggccgtcgt cggtgttggc ggcgacgggc gtcacctcgg cgtcggtgtc
    26101 cggcgtccag gacaggtccc gcaacctgcc gtcggcgtca cgcacgacga cgatcgcgtc
    26161 ctcggcgccg cgccccggca ggtcggcgtc acggaccgct gccccggcgg tggtcccggc
    26221 agcgaccctg atcggggctg ctagggcggg gctggcgggc gcgctcatca gtaattctcc
    26281 aaactttcag gggtgggtcg atcgcgacca tgctatcggt gtgagtcctc agtcccccac
    26341 gccaataggg ctcttcaacc agcggtgctc gatgccgacg agctcggccg cccaaccgag
    26401 cgctccgagc aaccacaagg tccccaggac cacggccgcg gtgaaggccg cgccgagtat
    26461 ctgcacccag agtccctgct tcttgaacca ggccatcacc ttgtcgtagc gctcgcgcgt
    26521 gtaggccagc agccttcgcg cccagtcgaa ttcggtggcc aggatcgcga gcccgaggaa
    26581 cacgatggcc cagcccggcc cgggatacgg gatcgcgagg atgccgacgc caagaaccgc
    26641 aagcccaacc actgcgatcg cgatgcggta gccgagctcg aatctggggc gctcgcgcag
    26701 ccggtcgcgc caccgcgccc agcgccgttt gg
//
LOCUS       DASRJA010000599         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2211873,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000599 DASRJA010000000
VERSION     DASRJA010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211873"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFC01_26650"
     CDS             complement(<1..203)
                     /locus_tag="VFC01_26650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HZN83231.1"
                     /translation="MTTTDKGMVNVPQNDQAESIAASELSEVFARQRAAFDRNPAPSL
                     QQRRATLLKLERLIEQNRLILAEA"
     gene            complement(285..1400)
                     /locus_tag="VFC01_26655"
     CDS             complement(285..1400)
                     /locus_tag="VFC01_26655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZN83232.1"
                     /translation="MTTSPNGKPAQRKKLRIAVVQPHLAVGAVEMNLRLCASLVREAS
                     VMHSPDVILLPEAMTSPNMFHRTMRRVPQPIDGAPYQMVVRLAKELHCTVGGGFLSVR
                     GQHARHTYVLAEPDGTTHLHDKDEPSMWETNFYTAGKDQGLTSTSFGNVGLAMGFEWG
                     RSRTARRLRNKVDVVLGGSCWWGGPTWPVMREWMARDHQYNALACGEAPTTMARMVGA
                     PVAVAQHVGRVDSDTPLLPGVGWSTMFSGDSQIVDGSGTIVARLGPEDYNAIACAEVE
                     VGPQEPIHPIPIGFWTQPAAGIVQGLWHYQKLHGRISYKVRHALGGFPWQHNPPTDLP
                     NYNPAGAPSQLALDDLPAESIEETITANAGDTKAQLT"
     gene            complement(1397..>2204)
                     /locus_tag="VFC01_26660"
     CDS             complement(1397..>2204)
                     /locus_tag="VFC01_26660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004572211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83233.1"
                     /translation="WAAKLAMLPRLRELVQGVNVQSKQRCTEDLPSYFRISSGPGWAL
                     VGDAGHFKDPVIAHGMHDAVLWATVLGDKLGPVFDKDDSTIDEAMRSYERERDAGVLP
                     SLYLAGRYTRTHSLAGPESELWKELETSPSFVQEFGNAYTRTLSLDRLFSFRRLARWS
                     ATSMIRAREDRAGVVRFSAAELRLKFGFLRDKAELALRGRARAAGARRWRDGWSDAVV
                     LERRTAVMRNAPRTTGYHGKGASRPVAFNEAVGLLTASNNHDNLASLETR"
BASE COUNT          387 a          713 c          677 g          427 t
ORIGIN      
        1 gcctcggcca gaatcagccg attctgctcg atcagccgct ccagcttgag cagggtggcc
       61 ctgcgctgct gcagcgacgg cgccggattg cggtcgaacg cggcgcgctg acgggcaaac
      121 acctcactga gttcactcgc agcaatcgat tccgcctgat cattttgtgg cacgttcacc
      181 atgcccttat cagtggtagt catcaacact ccttctctaa agcacttgct caaagcgcag
      241 cgatgccgca actccagccg gccagttcat ttcggcggcc tgtgtcatgt cagctgggcc
      301 ttcgtatcgc ccgcgttcgc ggtgatcgtt tcttcgatgg attcggcagg caggtcgtcg
      361 agtgcgagct gcgagggtgc gccggccggg ttgtagttcg gcagatcggt gggtggattg
      421 tgctgccagg ggaatccgcc cagtgcgtgc cgaaccttgt atgagatgcg gccgtgcagt
      481 ttctggtagt gccagaggcc ctggacgatt cccgccgccg gttgcgtcca gaagccgatg
      541 ggaatggggt ggatgggctc ctgtgggccg acttctactt cggcgcaggc gattgcgttg
      601 tagtcctcgg gacccaagcg cgcgacgata gtgcccgaac catcgacgat ctgcgagtcg
      661 ccactgaaca tggtcgacca cccgacaccg ggtagcaacg gcgtatcgga atcgaccctt
      721 cccacatgct gcgcgaccgc gactggagcg ccgaccatcc gggccatcgt ggtcggcgct
      781 tcaccgcacg ccagtgcgtt gtactgatgg tcgcgcgcca tccattcgcg catcaccggc
      841 catgtcggac ctccccacca acacgacccg cccaggacaa cgtcgacctt gttgcgtagc
      901 cgtctcgcgg tacgcgagcg cccccactcg aatcccattg ccaagccgac gtttccgaag
      961 ctggtggagg tcagtccctg gtctttgcct gcggtgtaga agttggtttc ccacatcgac
     1021 ggctcatctt tgtcatgcag gtgtgtcgtg ccgtcgggtt cggccaggac gtaggtgtgt
     1081 cgagcgtgtt gaccccgcac cgatagaaat cccccgccca ccgtgcagtg cagctcttta
     1141 gccagccgta cgaccatctg atacggagca ccatcgatgg gttggggaac tcgccgcatt
     1201 gtccggtgga acatgttggg tgacgtcatc gcctcgggca gcaagatgac gtccggcgaa
     1261 tgcatcaccg aggcttcccg gaccaacgaa gcgcaaaggc gaaggttcat ctccaccgcg
     1321 ccgacggcca gatgcggttg gacgacggcg attcgcagct tcttgcgctg cgctggcttg
     1381 ccgttaggtg atgtggtcat cgtgtctcca gactcgcgag gttgtcatgg ttattgctgg
     1441 cggtgagcaa gcccaccgcc tcgttgaagg ccaccggccg agaagcaccc ttcccgtgat
     1501 aaccggttgt ccgcggtgca ttccgcatga cggcggtgcg gcgttccagc acaaccgcat
     1561 ccgaccagcc gtcacgccag cggcgtgcgc cagcggcccg cgcccgcccg cgcagagcga
     1621 gttcggcctt gtccctgagg aacccgaact ttaggcgaag ttctgctgca ctgaaccgca
     1681 caacgccggc acggtcctcg cgtgcgcgga tcatcgatgt cgccgaccat cgcgcaaggc
     1741 gccgaaacga aaacagacgg tccaatgaga gagtgcgtgt gtacgcgttg ccgaactcct
     1801 gaacgaacga cgggcttgtt tccaattcct tccacagctc ggactctggc ccggcgaggg
     1861 aatgggtacg ggtgtaccgg cccgcgaggt acaggctggg caagacaccg gcatcacgct
     1921 ctcgctcgta ggagcgcatc gcctcatcga tggtgctgtc atccttgtcg aagacgggtc
     1981 cgagtttgtc gcccagcaca gtggcccaca ggaccgcgtc atgcatgccg tgggcgatca
     2041 ccggatcctt gaagtgcccg gcgtccccca ccagcgccca tcccggtccg gaggaaatcc
     2101 tgaagtaact cggtaagtct tcggtacacc gttgcttgga ctgcacattc accccctgca
     2161 ccagctcgcg cagccgaggc agcatggcca gcttggccgc ccac
//
LOCUS       DASRJA010000600         3983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4193168,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000600 DASRJA010000000
VERSION     DASRJA010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4193168"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFC01_26665"
     CDS             complement(<1..274)
                     /locus_tag="VFC01_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN83234.1"
                     /translation="MPMSRPTPPALTVRYEGSTRTFAPGNDVVVGRDLRADVRVAHPL
                     ISRAHLVLRFDQGRWVAIDNGSLNGMYVNGRRMPTADIHDGLQVNIG"
     gene            complement(324..722)
                     /locus_tag="VFC01_26670"
     CDS             complement(324..722)
                     /locus_tag="VFC01_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN83235.1"
                     /translation="MPTPTAVAQRLYDAIAHGDGHALFALLTDDFVGTVSTGMPHGVG
                     GEHRGAADMVTGVWSRIASEYDMHVDPHDYLQVDDERVVVLGRYWGAARDGGSAVDAA
                     FAHIITTREDRIAALQQITDTARWGVAPTS"
     gene            772..1332
                     /locus_tag="VFC01_26675"
     CDS             772..1332
                     /locus_tag="VFC01_26675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN83236.1"
                     /translation="MPRPALFTLDGLLDAAAALLAVDGPSAVTMSAVARAAGAPSGSM
                     YHRFPTRAALCGELWLRTEERFHAGVTAALATSADHQERCVAAARFTVQWCRDHPVEA
                     QVLLVGADALGAADWPDELISRRKRLHRALRRTLAGMAADPERVNAAVIDIPYAVVRR
                     HLMARHAIPPSADAIVEDCARALIRA"
     gene            complement(1329..2159)
                     /locus_tag="VFC01_26680"
     CDS             complement(1329..2159)
                     /locus_tag="VFC01_26680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN83237.1"
                     /translation="MKIGISTFVNDDSIDTVSLARAIEDRGFDSLVIAEHTHIPASRE
                     TPYPLGGELPPIYYRTLDPFVTLAAAAAVTSRIDLFTGIALQIQRDPIITAKEAASID
                     LISGGRFILGVGAGWNLEEMRNHGTNPKTRGALLDEQIEAIKVLWTDEPAEYHGKYVN
                     FDASYLRPKPVQKPYLPIYIGGDSDATVKRVIRHEAGWISNPFPVEHLKKRIDQMRDG
                     AGHDVPLAMFGTPLDPVYWRGVDDLGFGQVALLLPTLPTDESLRLLDEYAAKVDEYRG
                     "
     gene            complement(2443..3636)
                     /locus_tag="VFC01_26685"
     CDS             complement(2443..3636)
                     /locus_tag="VFC01_26685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HZN83238.1"
                     /translation="MNTQPNVFTPAKLGPVTLRNRIIKAATFEASTPNALVTEDLITY
                     HRLPAAGGVGMTTVAYLAVSPGGRTEGRQIWWRPEAVPGLQKLTAAIHAEGAAISAQI
                     GHAGPVADARSNKAKALAPVRFFNPLAMRFAKKATKDDIRDVIEAHADAARYAIECGF
                     DAVEIHLGHNYFASSFLSPLINRRADEFGGSLENRAKVARGMVLAVRRAVKDQIAVTA
                     KLNMSDGVRGGISTEESLQTAKWLEEDGGLDAIELTAGSSLVNPMYLFRGDAPVKEFA
                     GAFKPPLRWGIRMTGKKFFREYPYREAYLLRDAKLFRAELKMPLILLGGITNRETMDL
                     AMAEGFDFVAMGRALLAEPDLINRIQANDSVLSKCTHCNQCMATIYSHTHCVVTGSPD
                     RVTIG"
     gene            3665..>3983
                     /locus_tag="VFC01_26690"
     CDS             3665..>3983
                     /locus_tag="VFC01_26690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HZN83239.1"
                     /translation="MGAITLDGKATRDEIFVDLKERVAALTAAGRTPGLGTILVGDDP
                     GSHAYVRGKHADCAKVGINSIRRDLPADISQAELDATIDELNANPECTGYIVQLPLPK
                     HLNE"
BASE COUNT          647 a         1282 c         1331 g          723 t
ORIGIN      
        1 tgccgatgtt gacctgcagg ccgtcgtgaa tgtcggccgt tggcatccga cggccgttga
       61 catacatgcc gttgaggctg ccgttgtcga tcgcgaccca acggccctga tcgaaccgga
      121 gcaccaggtg ggcgcgcgag atcagcgggt gtgcaacgcg gacgtcagcg cgaaggtcgc
      181 ggcccaccac aacgtcgttg cccggcgcga acgtgcgcgt tgagccctcg tagcgaacag
      241 tcaacgcggg cggggttggt cggctcatcg gcaccgactc tatcggtccg ggggccggat
      301 gtagtgctgg tccgtgaggc gcgtcaggag gtcggcgcaa cgccccatct ggcggtgtcg
      361 gtgatctgct gcaacgccgc gatccgatcc tcgcgcgtgg tgatgatgtg ggcgaacgcg
      421 gcgtcgacgg ccgaaccacc gtcgcgggcc gcgccccagt accggccgag caccaccacc
      481 cgctcgtcgt cgacttgtag gtagtcgtgc ggatcgacgt gcatgtcgta ttcggatgcg
      541 atgcgcgacc agacgccggt gaccatgtcg gccgcgccgc gatgctcgcc gccgacaccg
      601 tgcggcatgc cggtgctgac ggtgccgacg aaatcgtctg tcaacaaagc gaatagcgca
      661 tggccgtcgc catgtgcaat cgcgtcgtag agccgttgcg ctactgcggt gggagtgggc
      721 atgagacctc caatcagtat tagagactac tctctactat aggattgggc aatgccgagg
      781 cctgcactgt tcacactcga cgggctgctc gatgccgccg cggcactact tgccgtcgac
      841 gggccgtccg cagtgacgat gtcggcggtg gcccgcgccg ctggcgcgcc gagcgggtcg
      901 atgtatcacc gcttccccac gcgcgccgcc ctgtgcggcg agctgtggct gcgcaccgag
      961 gagcggttcc acgccggcgt taccgccgcg ctcgccacat cggcagacca ccaggaacgg
     1021 tgcgtcgccg ccgctcggtt caccgtgcag tggtgccgcg atcacccggt cgaggctcag
     1081 gtgttgttgg tcggtgccga cgcgctcggc gccgcggact ggcccgacga actgatcagc
     1141 cgccgaaaac gtttgcaccg cgcgctgcgt cgaacactgg cgggcatggc cgcagacccg
     1201 gaacgggtca acgcggcggt gatcgatatt ccgtacgcgg tggtgcgcag gcacctgatg
     1261 gcgcggcacg ccatcccgcc gagcgcggac gcgatcgtcg aggattgcgc gcgggcgctt
     1321 attcgcgcct agccgcggta ttcatccacc tttgcggcat actcgtcgag cagtcgcagc
     1381 gactcgtccg tcggtagcgt cggcagcaaa agggcaacct ggccaaatcc caaatcatcg
     1441 acgccgcgcc agtagacggg gtccagcgga gtaccgaaca tggccaacgg aacgtcgtgg
     1501 ccggcgccgt cgcgcatctg atcgattcgc ttcttgaggt gttccacggg gaaggggttg
     1561 gaaatccagc cggcctcgtg cctgatcacg cgtttgactg tggcatcgga atcgccgccg
     1621 atgtagatcg gcaggtatgg cttctggacg ggcttgggcc tgaggtacga ggcgtcgaaa
     1681 ttgacgtact tgccgtggta ttcggccggc tcgtcggtcc acagcacctt gatggcttcg
     1741 atctgttcgt ccagtaatgc accgcgggtc ttggggttcg tgccgtggtt gcgcatctcc
     1801 tcgagattcc agccggcgcc cactcccaaa atgaaccggc cgccggagat caggtcgatg
     1861 ctcgcggcct ccttcgcggt gatgatggga tcgcgctgga tttgcagggc aattcctgtg
     1921 aagagatcga tcctggacgt caccgcggca gccgcggcca gcgtgacaaa cggatccagc
     1981 gtgcggtagt aaatcggcgg cagctcgcct ccgagtgggt acggagtttc gcggctcgcc
     2041 ggaatgtggg tgtgttcggc aataaccagt gagtcgaaac cccggtcctc gatcgcgcgc
     2101 gcgagtgaca ccgtgtcgat gctgtcgtcg ttgacgaacg tggagattcc gatcttcatt
     2161 gccatcctca tctgtcggct gtgcccggta ctgacatccg ctggagtgag cctattcccg
     2221 gccaactggc gctcataccg tctagttaac gtttggtgac ggcctcggcg cgtcgtgcgc
     2281 acagtccagc cacgtcgtga tctggtaagg catgccgtct gtgaaggcca ctgcccggcg
     2341 tcacggcaaa ctgggtcgag cgatgctccc ggtgttcgtc gtgtgtgccg cgctcatcat
     2401 caccctgctg atcgccaacc tgctcgtgta ggcggacggg gactagccga tcgtgacccg
     2461 gtcgggcgat cccgtgacca cgcagtgggt gtggctgtag atcgtcgcca tgcactggtt
     2521 gcaatgcgtg cactttgaga gcaccgaatc gttggcctgg atccggttga tcaggtccgg
     2581 ctcggccagc agcgcgcggc ccatcgcgac gaagtcgaag ccctcggcca tcgcgaggtc
     2641 catggtctcg cggttggtga tgccgcccag caggatcaat ggcatcttca gttcggcgcg
     2701 aaacagcttg gcgtcgcgca acagatacgc ctcgcggtac ggatactccc ggaagaactt
     2761 cttgccggtc atccgaatgc cccaccgcag cggcggtttg aacgcaccgg cgaattcctt
     2821 gaccggcgcg tcgccgcgga acaggtacat cggattgacg agtgaactgc ccgccgtcag
     2881 ttcgatggcg tccagtccgc catcctcttc gagccatttc gccgtctgca gcgattcttc
     2941 ggtcgagatg ccgccgcgca caccgtcgga catgttcagc ttcgcggtca ccgcgatctg
     3001 gtccttgacc gcacgccgca ccgccagcac catgccgcgg gccaccttgg cgcggttctc
     3061 gagcgacccg ccgaattcat cggcgcgacg gttgatcagc gggctgagga acgaactggc
     3121 gaaatagttg tggccgaggt ggatctcgac cgcgtcgaag ccgcattcga tcgcgtagcg
     3181 ggcggcgtcc gcgtgcgcct cgatgacgtc tctgatgtcg tctttggtgg ccttcttcgc
     3241 gaagcgcatc gcgagtgggt tgaagaaccg caccggcgcc agcgccttgg ccttgttgga
     3301 gcgggcatcg gcgaccgggc cggcgtggcc gatctgggcg ctgatcgcgg cgccctcggc
     3361 atggatcgcc gctgtgagct tctgcagacc cgggacggct tcgggccgcc accaaatctg
     3421 ccgaccctcg gtgcgcccgc ccggcgacac cgccaggtac gcgacggtcg tcatgccgac
     3481 gccgcctgcg gccggcaacc ggtgataggt gatcagatcc tcggtgacga gtgcgttcgg
     3541 cgtcgacgcc tcgaacgtcg ccgccttgat gatgcggttg cgcagcgtca ccgggccgag
     3601 cttggccggg gtgaacacat tgggctgagt gttcatacaa ggtgagcctg ccacaatggc
     3661 tgccgtgggt gcgatcacac tggacggcaa ggccacgcgc gacgagatct tcgtcgacct
     3721 caaggagcgg gtggcggcgc tgaccgcggc cggccggacg cccgggctgg gcaccatcct
     3781 ggtcggcgac gatcccggct cgcacgcgta cgtgcgcggc aagcacgccg attgcgcgaa
     3841 ggtcggcatc aactcgatcc gccgcgatct gcctgccgac atcagccagg ccgagctcga
     3901 cgcgaccatc gacgagctga acgccaatcc cgagtgcacc ggttacatcg tgcagctgcc
     3961 gctgcccaag cacctcaacg aga
//
LOCUS       DASRJA010000601        16115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_353718,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000601 DASRJA010000000
VERSION     DASRJA010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16115
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353718"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VFC01_26695"
     CDS             complement(<1..481)
                     /locus_tag="VFC01_26695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005254324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZN83240.1"
                     /translation="MIELKTAREIDKMAVTGEFVAETLATLSLEAQPGVNLMQLEHHA
                     RTLITDRGADSCYWDYAPSFGRGPFRNVICLSVNDAVLHGLPRDYVLRDGDVLSLDFA
                     VSIDGWVADSAVTVIVGDRADAGDRALVESTQRALDAGIAAAVPGGRLGDISAAIGEV
                     "
     gene            562..834
                     /locus_tag="VFC01_26700"
     CDS             562..834
                     /locus_tag="VFC01_26700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN83241.1"
                     /translation="MVRVPLSPEQVRAGQRLGALMRTARAGRDPEMIARNAGISPETL
                     RKIEVGRMPSPSFGTVIGLCNALGLPLQNAVDAWHGTDHIANGQPA"
     gene            969..1217
                     /locus_tag="VFC01_26705"
     CDS             969..1217
                     /locus_tag="VFC01_26705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83242.1"
                     /translation="MKIRTFVAPLLMAAGGAAAIAIAPLAQADATPPPCSFTGNASVC
                     QTEGNAQVSALPPTIDYQAQYPFGEYGGLLFHHRGRHG"
     gene            complement(1233..1305)
                     /locus_tag="VFC01_26710"
     tRNA            complement(1233..1305)
                     /locus_tag="VFC01_26710"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1270..1272),aa:Ala,seq:tgc)
     gene            complement(1434..1507)
                     /locus_tag="VFC01_26715"
     tRNA            complement(1434..1507)
                     /locus_tag="VFC01_26715"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1471..1473),aa:Ile,seq:gat)
     gene            complement(1565..2392)
                     /locus_tag="VFC01_26720"
     CDS             complement(1565..2392)
                     /locus_tag="VFC01_26720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HZN83243.1"
                     /translation="MSSPNEPGYPRAGEAPGSANGSPPHDGGSLGNAASRTGGDVPPW
                     QRGPAARAARQPQRQSPDAGHPPGVDARLNRFIGGGAGSPAPAPTHEQSAPPRGERTE
                     TVRPEAYASELPDLSGPMPRERKPAADRPAPEPARPASQSPGRIQVGTRQHKGPVRAS
                     MQIRRIDPWSTLKVSLVLSVALFFVWMIAVAFLYLVLGGMGVWSKLNSNVGDLLTSAS
                     GQTGGELVSSGTIFGGAALIGLVNIVLLTAMATCAAFIYNLTTDLVGGVEVTLADRD"
     gene            complement(2412..6176)
                     /gene="gyrA"
                     /locus_tag="VFC01_26725"
     CDS             complement(2412..6176)
                     /gene="gyrA"
                     /locus_tag="VFC01_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intein-containing DNA gyrase subunit A"
                     /protein_id="HZN83244.1"
                     /translation="MTDTTLPPGDEAGDRIEPVDIQQEMQRSYIDYAMSVIVGRALPE
                     VRDGLKPVHRRVLYAMYDSGFRPDRSHAKSARSVAETMGNYHPHGDASIYDTLVRMAQ
                     PWSLRYPLVDGQGNFGSPGNDPPAAMRYCVTGDALVRLPFGQSVRIADIVPGARPNSD
                     NVVDAKVLDRHGNPVVADRLFHSGDHQTYTVTTAEGYTVTGTANHPLLCLVDVAGVPT
                     LLWKFIEEVRPDDRVVIQRTPPTEFGPGDWHDTLEALLLGAFISEGFVSERRAGFNNL
                     DREYFNIVVAAYDAVVGGQRYVSERTIASGSNLLELDIHNLDAVRTSRLADLIGQRSA
                     DKVVPQWLWQSPAAVKRAFLQSLFEGDGSCSALSRNTIQISYSTRSGRLAKDVQQMLL
                     EFGVISRRYLHATGEHKVVITNRAHAEAFSAQIGFGGAKQTKLKALLQALPPKAAGLD
                     TDHVPGLAAFVRAHGGGRWVDKEWLRKHNIDRLSRWQRDSAEILSHISDSDVRAIASD
                     LTDGRFYYARVASVTDAGVQPVYSLRVDTEDHAFITNGFVSHNTEARLTPLAMEMLRE
                     IDEETVDFIPNYDGRVQEPTVLPSRFPNLLANGSGGIAVGMATNIPPHNLGELAEAVY
                     WCLENHEAEEEATLAAVTERVKGPDFPTSGLIVGSQGINDTYTTGRGSIRMRGVVEIE
                     EDSRGRTGIVITELPYQVNHDNFITSIAEQVRDGKLTGISNIEDQSSDRVGLRIVVEI
                     KRDAVAKVVLNNLYKHTQLQTSFGANMLSIVDGVPRTLRLDQLIRHYVDHQLDVIVRR
                     TTYRLRKANERAHILRGLVKALDALDEVIALIRASETVDVAKQGLIELLDIDEIQAQA
                     ILDMQLRRLAALERQKIVDDLAKIEAEIADLEDILAKPERQRGIVRDELKEIVDKHGD
                     ERRTRIVPADGEVSDEDLIAREDVVVTITETGYAKRTKTDLYRSQKRGGKGVQGAGLK
                     QDDIVNHFFVCSTHDWILFFTTQGRVYRAKAYDLPEASRTARGQHVANLLAFQPEERI
                     AQVIQIKSYEDAPYLVLATRNGLVKKSKLTDFDSNRSGGIVAVNLRDGDELVGAVLCS
                     AEDDLLLVSAKGQSIRFSATDEALRPMGRATSGVQGMRFNEEDKLLSLNVVQPDTYLL
                     VATAGGYSKRTAIDEYPVQGRGGKGVLTIQYDKRRGTLVGALIVDDDTELYAITSGGG
                     VIRTAARQVRKAGRQTKGVRLMNLGEGDTLVAVARNAEENEADAAEADAT"
     gene            complement(6187..8214)
                     /gene="gyrB"
                     /locus_tag="VFC01_26730"
     CDS             complement(6187..8214)
                     /gene="gyrB"
                     /locus_tag="VFC01_26730"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HZN83245.1"
                     /translation="MAAAKKKAQQEYGAQSITILEGLEAVRKRPGMYIGSTGERGLHH
                     LIWEVVDNSVDEAMAGYASRVDVRLLEDGGVEVTDDGRGIPVAMHASGVPTVDVVMTQ
                     LHAGGKFDSDSYAVSGGLHGVGVSVVNALSSRLEVEIKRDGHEWFQYYDQSVPGTLKQ
                     GEATKKTGSTVRFWADPNIFETTNYDFETVARRLQEMAFLNKGLTITLVDERVTAAEV
                     VDEVVSDTAEAPKSAEEKAAEAAGPHKVKNRTFHYPGGLVDYVKHINRTKTAIHQSIV
                     DFSGKGEGHEVEFAMQWNAGYSESVHTFANTINTHEGGTHEEGFRAALTSVVNKYAKD
                     KKLLKEKDANLTGDDIREGLAAVISVKVAEPQFEGQTKTKLGNTEVKSFVQRICNEQL
                     GHWFEANPAEAKTVVNKAVSSAQARIAARKARELVRRKSATDIGGLPGKLADCRSTDP
                     RKSELYVVEGDSAGGSAKSGRDSMFQAILPLRGKIINVEKARIDRVLKNTEVQAIITA
                     LGTGIHDEFDITKLRYHKIVLMADADVDGQHISTLLLTLLFRFMKPLIENGHVFLAQP
                     PLYKLKWQRSEPEFAYSDRERDGLLEAGKKAGKKINVDDGIQRYKGLGEMDAKELWET
                     TMDPSVRVLRQVTLDDAAAADELFSILMGEDVEARRSFITRNAKDVRFLDV"
     gene            complement(8438..9007)
                     /locus_tag="VFC01_26735"
     CDS             complement(8438..9007)
                     /locus_tag="VFC01_26735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 family protein"
                     /protein_id="HZN83246.1"
                     /translation="MTNDDEDDDIETGPPAHLAGLAGMDLVRRTLEEARGAARVQGKD
                     VGRGRSVPVHRRVAGISRRRWSGPGPDSRDPQPLGSATRDLARARGWSGRVAEGSVFG
                     RWTAVVGEQIAAHATPTALNDGVLTVSAESTAWATQLRMVQAQLLAKIATAVGDGVVT
                     SLKIVGPVAPSWRKGRYHIAGRGPRDTYG"
     gene            complement(9082..10242)
                     /gene="recF"
                     /locus_tag="VFC01_26740"
     CDS             complement(9082..10242)
                     /gene="recF"
                     /locus_tag="VFC01_26740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HZN83247.1"
                     /translation="MHVRRLALTDFRSWPRIELELEPGRTVFVAPNGFGKTNLVEALW
                     YSSTLGSYRVASDAPLIRAGAERAVVSTIVVNDGRELAVDLEITAGRANKARLNRSPV
                     RSAREILGVLRAVLFAPEDLALVRGDPGERRRYLDELATTRRPTVGAVRADYDRVLRQ
                     RAALLKTAGAARHRGDRSVLDTLDVWDGHLAAHGAQLIAARVELVTQLAPEVEKAYQL
                     LAPSSRPAAIQYRSAVEVVENEAAAGNDSVEVFEAALLDALARRRDAELERGVCLVGP
                     HRDDLELRLGDQVAKGFASHGESWSMALALRLAAYELLRADGGDPVLLLDDVFAELDN
                     ARRQALANVAASAEQVLVTAAVHDDIPAGWDARRIEITMRDDDTGRISMVQP"
     gene            complement(10243..11148)
                     /gene="gnd"
                     /locus_tag="VFC01_26745"
                     /pseudo
     CDS             complement(10243..11148)
                     /gene="gnd"
                     /locus_tag="VFC01_26745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005087665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
     gene            complement(11168..12367)
                     /gene="dnaN"
                     /locus_tag="VFC01_26750"
     CDS             complement(11168..12367)
                     /gene="dnaN"
                     /locus_tag="VFC01_26750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZN83248.1"
                     /translation="MDVATTTVGLTDLKFRLVREDFADAVAWVARNLPTRPTVPVLAG
                     VLLTGTDDGLTISGFDYEVSAEVQVAAEIASPGSVLVSGRLLSDITRALPAKPVDVSV
                     EGTRVSLTCGSARFSLPTMAVEDYPALPALPEDTGVISSELFAEAIGQVAVAAGRDDT
                     LPMLTGIRVEISGEKVVLAATDRFRLAVRELTWSTESADIEAAVLVPAKTLAEAAKAG
                     ADGADVHLSLGAGAAVGKEGLLGIRSDGKRSTTRLLDAEFPKFRQLLPTEHTAIATIG
                     VAELTEAIKRVALVADRGAQVRMEFADDVLRLSAGADDVGRAEEDLPVAFSGDPLTIA
                     FNPTYLTDGLGSLHSDRVTFGFTTPSRPAVLRPAGEDDGSVGATGPFPAAQTDYVYLL
                     MPVRLPG"
     gene            complement(12894..14396)
                     /gene="dnaA"
                     /locus_tag="VFC01_26755"
     CDS             complement(12894..14396)
                     /gene="dnaA"
                     /locus_tag="VFC01_26755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZN83249.1"
                     /translation="MTADPDPPFVAVWNNVVAELNGESDTGEIHNGDSSLPTLTPQQR
                     AWLKLVQPLVITEGFALLSVPTPFVQNEIERHLREPIITALSRQLGKRVELGVRIAAP
                     TSDASEESLNGFTPAAVSPEPDEVDEDSEALASAEESWPTYFSSRLQNTPSGEPTAVN
                     LNRRYTFDTFVIGASNRFAHAATLAIAEAPARAYNPLFIWGESGLGKTHLLHAAGNYA
                     QRLFPGMRVKYVSTEEFTNDFINSLRDDRKASFKRSYRDIDVLLVDDIQFIEGKEGIQ
                     EEFFHTFNTLHNSNKQIVISSDRPPKQLATLEDRLRTRFEWGLITDVQPPELETRIAI
                     LRKKAQMDRLDVPGDVLELIASSIERNIRELEGALIRVTAFASLNKTPIDKALAEIVL
                     RDLIADATTMQISTAAIMAATAEYFETTVEELRGPGKTRALAQSRQIAMYLCRELTDL
                     SLPKIGQAFGRDHTTVMYAEKKIRGEMAERREVFDHVKELTTRIRQRSKR"
     gene            14949..15092
                     /gene="rpmH"
                     /locus_tag="VFC01_26760"
     CDS             14949..15092
                     /gene="rpmH"
                     /locus_tag="VFC01_26760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZN83250.1"
                     /translation="MAKGKRTFQPNNRRRARVHGFRLRMRTRAGRAIVSNRRGKGRRK
                     LTA"
     gene            15114..15470
                     /gene="rnpA"
                     /locus_tag="VFC01_26765"
     CDS             15114..15470
                     /gene="rnpA"
                     /locus_tag="VFC01_26765"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZN83251.1"
                     /translation="MLPARYRMTRSTEFGATVSHGVRAAQPDLVVHALRSPGGGDGPR
                     IGLVVAKSVGSAVQRHRVARRLRHVARTVIDELEPADRVVIRALPSSRHAISARLEQE
                     LRTAIRRTRPRSGSSR"
     gene            15467..15787
                     /gene="yidD"
                     /locus_tag="VFC01_26770"
     CDS             15467..15787
                     /gene="yidD"
                     /locus_tag="VFC01_26770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZN83252.1"
                     /translation="MSAAQVGRNGSPVVRGVVFVIELYRHMISPMRLPTCRFTPTCSQ
                     YAVDALTEYGLVRGGWLALLRLLKCGPWHRGGWDPIPERRSAVPDDRECGRAADDLES
                     EIRV"
     gene            15732..>16115
                     /gene="yidC"
                     /locus_tag="VFC01_26775"
     CDS             15732..>16115
                     /gene="yidC"
                     /locus_tag="VFC01_26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZN83253.1"
                     /translation="MTVSADVPLTIWKARFVFNWFSLDVIYYPVSAIMWVWYKAFAFV
                     LGPANFFAWGLSVMFLVFTLRAILYKPFVRQIRTTRQMQELQPQIKALQKKYGKDRQR
                     MALEMQKLQKEHGFNPILGCLPMLAQ"
BASE COUNT         2701 a         5219 c         5210 g         2985 t
ORIGIN      
        1 cgacttcgcc gattgcggcg gagatgtcac cgagacgacc gccggggacc gctgcggcga
       61 taccggcgtc aagggctctt tgcgtcgact cgaccagggc tctgtcaccg gcgtcggctc
      121 tgtcaccgac gatgaccgtg accgcggaat cggccaccca accgtcgatc gacaccgcaa
      181 agtccagact caacacgtcg ccatcacgta gcacgtagtc gcgtggaagg ccatgcagca
      241 cagcgtcgtt gaccgacaga cagatgacat tccggaatgg accgcgaccg aatgacggcg
      301 cgtagtccca gtagcacgag tccgcaccgc ggtcggtaat cagcgtgcgg gcatggtgct
      361 cgagctgcat caggttcact ccgggctgcg cctcgagcga cagcgtcgcc agcgtttccg
      421 cgacgaattc accagtcacc gccatcttgt cgatctctcg agcggtcttg agttcgatca
      481 tgctgggcct tccgatcggt atttttatac cgtagcgacg gtaccagtac cggtacttaa
      541 ataccggcta tgctgaccga catggtgcgt gtacctctga gcccagagca ggtgcgagcc
      601 gggcaacgtc tcggcgcgct gatgaggaca gcccgagccg gtcgcgaccc cgagatgatc
      661 gcgcgcaacg ccggcatctc gccggagaca ctgcgcaaga ttgaggtcgg cagaatgccc
      721 agcccgagct tcggcacagt gattggactc tgcaacgcgc tcggcttgcc cctgcagaat
      781 gccgtcgatg cctggcatgg gacagaccac atcgccaatg ggcagcccgc ctgagcacaa
      841 tgcgcaaaag ctgataatcg cctgttgtgc gggttgtgcg cacaacagag ccaacggcta
      901 cctcgggcac atagcttcgg ccgcaagcaa gttcaccccg aactgctcgc acaagaaagg
      961 tcgaactaat gaaaatccgg acattcgttg ctccgctgct aatggcagca ggtggcgctg
     1021 ccgcaatcgc gatcgcgccg ctggcgcagg cggacgccac tccaccgccg tgcagcttca
     1081 ctggtaacgc gagcgtctgc cagacggagg ggaacgcaca ggtgagtgcc ttgccgccca
     1141 cgatcgatta ccaggcgcag tacccattcg gcgagtacgg cggactgctg ttccatcacc
     1201 gaggccggca tggctgaaat cgtgcggggg agtggagcct aggagaatcg aactcctgac
     1261 atctaccttg caaaggtagc gctctaccaa ctgagctaag gcccctgatt gatcaggatg
     1321 gagcgtcggc cgctgcatgc cacacctcga cgccgtgtcg cgatcgccag acgatcacgg
     1381 cgatcgcgac tgctgcgaac agcaccacga gcctcatggt gtggaccttt ccgtggggct
     1441 aggaggactc gaacctccga cctcttcgtt atcagcgaag cgctctaacc gcctgagcta
     1501 tagccccgta atcgcgcacg gaccgagcga cgagactacc gcaatcgtaa gctgcggccc
     1561 aaactcaatc ccgatccgcc agtgtgacct cgaccccgcc gaccaggtcg gtggtgaggt
     1621 tgtagatgaa ggccgcacac gtggccatcg cggtcagcag cacgatgttc accaacccga
     1681 tcaatgccgc gccgccgaag atcgtcccgc tggacaccag ctcgccgccg gtctgcccgc
     1741 tggcgctggt gagcaggtcg ccgacattgc tgttgagctt gctccacacg cccatgccgc
     1801 cgagcaccaa gtacagaaag gcgacggcga tcatccacac gaagaacaac gcaaccgaga
     1861 gcaccagcga caccttgagc gtgctccacg ggtcgatccg ccggatctgc atgctggccc
     1921 gcaccgggcc tttgtgttgc cgggtgccga cctggatgcg gccggggctc tgactcgccg
     1981 gccgagccgg ttcaggcgcg ggccggtcgg ccgccggctt gcgctctcga ggcatcggtc
     2041 cggacaggtc aggcagctcg ctggcatacg cctcgggccg cacggtctcc gtgcgctccc
     2101 cgcgtggcgg ggcgctctgc tcgtgcgtcg gcgccggcgc cggcgatccc gctccgccgc
     2161 cgatgaagcg gttcagccgg gcatccacgc ccggggggtg accggcgtcc ggcgactggc
     2221 gctgtggctg ccgagccgcc cgcgccgccg gaccccgctg ccaagggggg acgtcgccgc
     2281 cggtgcgcga cgccgcgttg ccgagcgaac caccatcgtg cggcggggag ccgttggcgc
     2341 taccgggggc ttcacccgcg cgcgggtatc ccggctcgtt cggtgaactc acctacggct
     2401 ccttaccgtc gttacgttgc gtcggcctcg gcggcgtcgg cttcgttctc ctcggcgttg
     2461 cgcgcaacag caaccagtgt gtcgccctcg cccaggttca tcaaccgaac gcccttggtc
     2521 tgccgccccg ccttgcggac ctggcgagcc gccgtccgga tgacgccccc accggaggtg
     2581 atggcgtaca actcggtgtc gtcatcgacg atcaacgctc cgaccagagt gccacgacgc
     2641 ttgtcgtact ggatcgtcag cacccctttg ccgccgcggc cctggaccgg gtactcgtca
     2701 atcgcggtcc gcttggagta gccgccggcc gtcgcgacca gcagatacgt gtcgggttgc
     2761 acgacattca gcgagagcag cttgtcctcc tcgttgaacc gcatgccctg cacaccggag
     2821 gtggcccgac ccatcggacg tagggcctcg tcggtagctg agaaccggat cgactggccc
     2881 ttggcagaca ccaacagtag gtcgtcctcg gccgaacaca gcaccgcgcc gaccaattcg
     2941 tcgccgtcgc gcaggttcac cgcgacgatg ccgcccgagc ggttggagtc gaagtcggtg
     3001 agcttggact tcttcaccag cccgttgcgg gtggccagca cgaggtatgg cgcgtcctcg
     3061 tagctcttga tctggatgac ctgcgcgatg cgctcctcag gctggaaggc cagcaggttc
     3121 gcgacgtgct gaccgcgcgc cgtgcgcgag gcctcgggca ggtcgtacgc cttggcgcgg
     3181 tacacccggc cctgcgtggt gaagaacagg atccagtcgt gcgtcgagca cacgaagaag
     3241 tggttgacga tgtcgtcctg cttgagaccg gcgccctgca cgcccttacc gccacgcttc
     3301 tggctgcggt agaggtcagt cttggtgcgc ttggcgtacc ccgtctcggt gatggtgacg
     3361 acgacgtcct cgcgcgcgat caggtcctcg tcggaaactt cgccatcggc cgggacgatg
     3421 cgggtgcgac gttcgtcacc gtgcttgtcg acgatctcct tgagctcgtc gcgcacgatg
     3481 ccgcgctgac gctccggctt ggccaggatg tcttccaggt cggcgatctc ggcctcgatc
     3541 ttggccagat cgtcgacgat cttctgccgt tccagggcgg ccaggcgacg caactgcatg
     3601 tcgaggatcg cctgggcctg aatctcgtcg atgtcgagca gctcgatcag gccttgcttg
     3661 gcgacgtcga ccgtctcgga tgcccggatc aacgcgatga cttcgtcgag cgcgtcgagc
     3721 gccttgacca gaccgcgcag gatgtgggcc cgctcgttgg ctttgcgcag ccggtaggtg
     3781 gtgcgccgga cgatgacgtc gagctgatgg tcgacgtagt gccggatcag ctggtcgagc
     3841 cgcagcgtgc gcggcacgcc gtcgacgatc gacagcatat tggcgccgaa gctcgtctgc
     3901 agctgggtgt gcttgtagag gttgttcagg accaccttcg cgaccgcatc gcgtttgatc
     3961 tcgaccacaa ttcgcaggcc gacgcggtcg ctcgactgat cctcgatgtt cgagataccg
     4021 gtcagctttc cgtcgcggac ctgttctgcg atcgaggtga tgaagttgtc gtggttgacc
     4081 tgatacggca gctcggtgat gacgatcccg gtgcggccgc gcgagtcctc ctcgatctcc
     4141 accacaccgc gcatacgaat ggatccgcgc ccggtggtgt aggtgtcgtt gatcccctgc
     4201 gacccgacga tcaggcccga agtggggaag tcggggccct tgacccgttc ggtgaccgcg
     4261 gccagggtgg cctcttcctc ggcctcgtgg ttctccaggc accagtacac cgcttcggcg
     4321 agctcgccga gattgtgcgg cggaatgttg gtggccatgc cgaccgcgat accgcccgaa
     4381 ccgttggcca acagattcgg gaaccggctc ggcagaaccg tcggctcctg cacgcgcccg
     4441 tcgtagttcg ggatgaaatc gactgtctcc tcgtcgattt cacgcagcat ctccatcgca
     4501 agcggagtga gccgcgcttc cgtgttgtgg ctgacgaagc cgttggtgat gaaggcgtgg
     4561 tcttccgtgt cgacacggag gctgtagacc ggttgcacgc cagcgtcggt cactgaggcc
     4621 acgcgcgcgt agtagaaccg cccgtcggtc aggtccgaag cgatcgcgcg gacgtcggag
     4681 tcgctgatgt ggctgaggat ctcagcgctg tcgcgctgcc accgggacag ccggtcgatg
     4741 ttgtgcttgc gcaaccactc tttgtcgacc cagcgtccgc cgccgtgcgc acgaacgaag
     4801 gcggcgaggc ccggaacatg gtcggtgtcc aagcctgcag cctttggagg caaggcttgt
     4861 agcagcgcct tgagcttcgt ctgcttagcc ccgccgaagc cgatctgggc cgagaacgcc
     4921 tcggcatgag cacggttagt gatgacgacc ttgtgctcgc ccgtcgcgtg caggtaacgg
     4981 cgcgagatca cgccgaattc cagcagcatc tgctggacat ccttcgcgag acgcccgctg
     5041 cgcgtggagt acgagatctg aatcgtattg cgcgacagcg cagaacagga tccgtcgcct
     5101 tcgaacaagg actgcaggaa tgcgcgcttg actgcggccg gggattgcca gagccattgc
     5161 ggcacgacct tgtccgccga ccgctggccg atgaggtcgg cgagccgact cgtccgcacg
     5221 gcgtccagat tgtgaatatc gagctcaagg aggttcgatc ccgaagcgat cgttcgctcc
     5281 gagacatagc gctgaccacc aactacggcg tcgtacgcgg caacgacgat gttgaagtat
     5341 tcgcggtcga ggttgttgaa gccagcgcgc cgctccgaga caaatccctc gctgatgaag
     5401 gcgcccaaga ggagcgcctc cagcgtgtcg tgccagtctc cagggccgaa ctcggtcggc
     5461 ggtgtcctct ggatcaccac gcggtcgtcc ggccgtacct cttcgatgaa cttccacaac
     5521 aaggtcggca cgccggccac gtcgaccagg cacagcaggg gatggttggc ggtacccgtg
     5581 accgtatatc cctcggccgt cgtcaccgtg taggtctgat gatcgccgga gtggaacagg
     5641 cgatcggcca ccactgggtt gccgtgccgg tccaacacct tcgcgtcaac gacgttgtcg
     5701 gagttcggtc gtgctccagg aacgatgtcg gcgatccgga cggactgccc aaacggcaaa
     5761 cgcaccaatg catcacccgt gacgcagtac ctcatcgccg ccggtggatc gtttccgggc
     5821 gagccgaagt tgccttgacc gtcgaccaac ggatagcgca gcgaccacgg ctgcgccatc
     5881 cggactaggg tgtcgtagat cgacgcatcg ccgtgcggat ggtagttacc catcgtttcg
     5941 gcaacggagc gcgccgattt cgcgtggctg cggtccggcc ggaagcccga gtcgtacatc
     6001 gcgtagagca cccggcggtg caccggcttg aggccgtcgc ggacctccgg cagcgcacgg
     6061 ccgacgatga cgctcatcgc atagtcgatg tagctgcgct gcatctcctg ctgaatgtcg
     6121 accggctcga tgcggtctcc ggcttcgtcg ccgggcggca acgtggtatc agtcattgaa
     6181 tttgtcctaa acgtctagga agcgaacgtc tttggcgttc cgggtgataa agctgcggcg
     6241 tgcctcgacg tcctcgccca tcaggatcga gaacagctcg tcggccgccg ccgcgtcgtc
     6301 caaagtcacc tgtcgcaaca cgcgcaccga cggatccatc gtcgtttccc acagctcctt
     6361 ggcgtccatc tcgccaagac ccttgtaccg ctggatgccg tcgtcgacgt tgatcttctt
     6421 gccggccttc ttgccggcct cgagcagtcc gtcgcgctca cggtcggaat acgcgaactc
     6481 cggctcgctg cgctgccatt tcaacttgta cagcggcggc tgcgccagga acacgtggcc
     6541 gttttcgata agcggcttca tgaatcggaa cagcagcgtc aacaacaggg tcgagatgtg
     6601 ctggccgtcc acgtcggcgt cggccatcag cacgatcttg tggtagcgca gcttggtgat
     6661 gtcgaactcg tcgtgaatgc ccgtacccag cgcggtgatg atcgcctgga cctcggtgtt
     6721 cttcagcacc cggtcgatgc gggccttctc gacgttgatg atcttgccgc gcaacggcag
     6781 gatcgcctgg aacatcgagt cgcggccgct cttggccgag ccgccggccg agtcgccctc
     6841 caccacatac agttccgact tgcgcggatc agtggagcgg cagtccgcca gcttgccggg
     6901 cagcccgccg atgtcggtgg cgctcttgcg gcgcaccagc tcgcgggcct tgcgcgccgc
     6961 gatgcgcgcc tgtgccgacg aaaccgcctt gttcacaacc gttttcgcct cggccggatt
     7021 ggcctcgaac cagtggccca gctgctcgtt gcaaatgcgc tgcacgaacg acttcacctc
     7081 ggtgttgccg agcttggttt tcgtctgacc ctcgaactgc ggctcggcca ccttcaccga
     7141 gatgactgcc gccagccctt cacggatgtc gtcgccggta aggttcgcgt ccttctcctt
     7201 gagcagcttc ttgtccttgg cgtacttgtt caccacgctc gtcagcgccg ctcggaaccc
     7261 ctcttcatgg gtgccgccct cgtgggtgtt gatcgtgttg gcgaaggtgt gcaccgactc
     7321 cgagtagccg gcgttccact gcatcgcgaa ctcgacctcg tggccctctc ccttgccgga
     7381 gaagtcgacg atgctctggt ggatcgcggt cttggtgcgg ttgatgtgct tgacgtagtc
     7441 gaccagcccg cccgggtagt ggaacgtgcg gttcttcacc ttgtgcgggc cggccgcctc
     7501 ggcagccttc tcctcggcag acttcggcgc ctcggcggta tcactgacga cctcgtcgac
     7561 cacttctgct gcggtcaccc gctcatcgac cagcgtgatg gtcagcccct tgttcaggaa
     7621 cgccatctcc tgcagccgtc gtgccacggt ctcgaagtcg tagttcgtgg tctcgaaaat
     7681 gttcggatct gcccagaacc gcaccgtcga accggtcttc ttggtggcct cgccctgctt
     7741 cagcgtgccg ggcaccgact ggtcgtagta ctggaaccac tcatggccgt cgcgcttgat
     7801 ctccacctcg aggcgggagg acagcgcgtt gaccacggag acgcccacgc cgtgcaggcc
     7861 gcccgacact gcataggagt cggagtcgaa cttgccgccg gcgtgcaact gcgtcatgac
     7921 gacgtcgacg gtcggtacac cggaggcgtg catggcgacc gggatgccgc ggccgtcgtc
     7981 ggtgacctcg acgccaccgt cctcgagcag ccgcacgtcc acgcggctgg cgtagccggc
     8041 catcgcctcg tcgacggagt tgtcgaccac ctcccagatg aggtggtgca gaccgcgctc
     8101 gcccgtggag ccgatataca tgcccggacg tttgcggaca gcctctagcc cctcgagaat
     8161 cgtgatggac tgagcgccgt attcctgttg tgctttcttc ttcgcagcag ccacgggcgg
     8221 aagggtctcc ttgggggtcg cagcggcggt agtcgttacc gccggcaagt catgaggaca
     8281 gtctaccggg aagtagcgcc aggaccgatt ctgaggcggc gtttctacac acctatttgc
     8341 gtcgcctgtg gaatttctcg atccgaggtg cgtcaagacg cttgcgagct aagatcgcgg
     8401 ctttctggcg gctctcaggg aatgagccgg taggccgcta tccgtacgtg tcgcggggcc
     8461 cgcggccagc gatgtgatag cgccccttgc gccacgacgg agccaccgga ccgacgattt
     8521 tcagcgatgt cactaccccg tcgccgaccg ccgtcgcgat cttggctaac agttgcgcct
     8581 gcaccatccg cagctgcgtc gcccatgcgg tcgattcggc ggacaccgtg agcactccgt
     8641 cgttcagcgc ggtcggtgtg gcgtgcgcgg cgatctgctc cccaaccacc gctgtccagc
     8701 ggccgaaaac cgagccctcg gcaacccgcc cggaccaacc gcgggcgcgc gccaggtcgc
     8761 gcgtcgctga cccgagcggc tgcggatcgc gcgaatccgg gcccgggccc gaccaccgtc
     8821 gtcggctgat cccagccacc cggcggtgga ccggcacgct gcggccacgc cccacgtcct
     8881 taccctgcac ccgcgccgct ccgcgcgcct cctcgagcgt gcgccgtacc aggtccatcc
     8941 cggcaaggcc ggctaagtgg gcgggcggtc ctgtttcgat gtcgtcgtcc tcgtcgtcgt
     9001 tagtcattgg ctcgactccc cgctcgctcc ggtcctcgct cgtccctcgc tgcgatcctc
     9061 actcgccttc gtcttcagaa gtcatggctg caccatcgaa atccggccgg tgtcgtcgtc
     9121 gcgcatggtg atctcgatcc ggcgcgcatc ccagccggcg ggaatgtcgt cgtgcaccgc
     9181 ggcggtgacc agcacctgtt ccgcggacgc ggccacatta gccagcgcct gccgacgcgc
     9241 gttgtcgagt tcggcgaaca cgtcgtccag cagcaacacc gggtcgccgc cgtccgcccg
     9301 cagcagctcg tacgcggcga gccgcagcgc caacgccatc gaccacgatt ctccgtggct
     9361 tgcaaagcct ttcgcgacct gatcgcccag ccgcagctcg aggtcgtcgc ggtgcgggcc
     9421 gaccaggcat accccgcgct cgagttccgc gtcgcggcgc cgggccagcg cgtcgagcag
     9481 cgcagcctcg aacacctcca cgctgtcgtt gcccgcggcc gcctcgttct cgaccacctc
     9541 gacggcgctg cggtactgga tggcggccgg tcgtgacgac ggcgccagca gctggtaggc
     9601 cttttccacc tcgggggcca gttgagtgac cagctcgacg cgcgccgcga tcagttgggc
     9661 gccatgcgcg gcgaggtgcc cgtcccagac atcgagggtg tcgagcacac tgcggtcccc
     9721 gcggtggcgg gcagcgccgg cggtcttgag cagcgccgcg cgctgtcgca gcactctgtc
     9781 gtagtctgcg cgcaccgcgc cgacggtggg ccgtcttgtg gtggccagtt cgtcaaggta
     9841 gcggcggcgc tcgccgggat cgccacgtac cagcgccagg tcctcgggcg cgaacagcac
     9901 ggcccgcaag acaccgagga tttcacgggc cgagcggacg ggggagcggt tcaaccgcgc
     9961 cttgttggcc cgtcctgcgg tgatttcgag gtcgacggcc aattcgcggc cgtcgttcac
    10021 cacgatcgtc gacaccaccg cacgctcggc acccgcccgg atcagcggtg cgtccgacgc
    10081 tacgcgatac gagcctagcg tcgacgaata ccacagcgcc tcaacgagat tcgtcttacc
    10141 aaagccgttc ggggccacga agaccgtccg gccgggctcc agttcgagtt cgatccgcgg
    10201 ccacgagcgg aagtcagtca gcgcgaggcg tcggacgtgc acttagccgg actcgctgat
    10261 cctcttgacg gcgtggccgc cgaactggtt ccgcagcgca gacacggcct tcatcgacgg
    10321 tgagtcattc tggcgggacg cgaaccgcgc gaacaacgag gcggcgatga cgggcatcgg
    10381 cacgcgatgg ttgatggcct cctcgaccgt ccaccggcct tcgccagaat cctcggtgta
    10441 gccgctgatc tcgacgaatt tcgggtcttc cttcagcgcc ttggccagta gctgctgcag
    10501 ccaggaccga accaccgtgc cgttggtcca agcctggatg acggcctggg tgtcagtgat
    10561 caggtcctcg gccgccaaca actcatagcc ctcggcgtag gccatcatga ggccgtactc
    10621 gatgccgttg tgcaccatct tggcgtagtg cccggcgccg accggtccag catgaacaaa
    10681 cccgtcttcg cgcggccctg gcggacgcag ggtgtcgaag atcggcattg cccgttcgat
    10741 gtcggcattg ctgcccccca ccatcaggcc atagccttcc ttcaatcccc agacgccgcc
    10801 cgacacaccg gcatcgatga acgctattcc cttgtcgccc aacagttttg cgtgtgggcc
    10861 gtcctcggtg tagcgggagt tgccgccatc gatcaccatg tcaccaggac tgagtacatc
    10921 ggcgagcgac gcgatggttt cgtgggtaat cggaccggac gggaccatca cccacacgac
    10981 ccgcggcgcg ttcagcgctt cggcaagcgc cgcgagcgtc ggcacatcgg tgacctccgg
    11041 acgcgggtcg tagccgatca cctcatgcgc gccctcacgc aggcgttccc gcatgttgaa
    11101 acccattttg cccaggccga cgagacctaa ctgcatgtgc gcgcctcttt cgtggcggta
    11161 gggccggtca gcccggaagc cgcaccggca tcaacaggta gacatagtcg gtttgagcgg
    11221 ccgggaacgg gccggttgcg ccgacactcc catcatcctc accggccgga cgcaacaccg
    11281 cgggacggct gggtgtcgtg aaaccgaacg tcacgcggtc cgagtgcaac gagcccagcc
    11341 cgtccgtcag gtacgtcggg ttgaatgcga tggtcaacgg gtcacccgag aaggcgacgg
    11401 gaagatcctc ttcagcgcgg ccgacatcgt cggcgcctgc cgacaaccgc aacacgtcgt
    11461 cggcgaattc cattcgcacc tgcgcgccgc ggtccgccac cagcgcgaca cgcttgatcg
    11521 cctcggtgag ctcggcaaca ccgatggtcg cgatcgccgt gtgctcggtg ggcaacagct
    11581 ggcggaactt cgggaactcg gcgtccagca ggcgagtggt gctccgcttg ccgtcgctgc
    11641 ggatgccgag caggccctcc ttgccgaccg ccgccccggc gcccaacgac aaatgcacgt
    11701 ccgcgccgtc ggccccggct ttcgcggctt cggccaaagt cttggcgggc accagcaccg
    11761 cggcctcgat gtccgcggat tcggtcgacc acgtcagctc gcgtaccgcc aggcggaaac
    11821 ggtcggtggc cgccaaaacc accttctcgc cggaaatctc gacacggatg ccggtcagca
    11881 tcgggagcgt gtcatcacgc ccggcggcaa cggcgacctg gccgatggcc tcggcgaaca
    11941 gctcagacga gatgacaccc gtgtcctcgg gcaaggccgg cagcgccggg taatcctcga
    12001 ccgccatggt gggcagcgag aaccgcgcgc tgccgcaggt caatgacacc cgggtgcctt
    12061 cgacgctgac atcgacgggt ttggcaggca gtgcccgggt gatgtcggac aacaaccgac
    12121 cggacaccaa aacgcttcca ggagaagcga tttcggctgc aacctgaact tcggccgaca
    12181 cttcgtagtc gaatccggag atcgtcaacc cgtcgtcagt gccggtaagt aacacgccgg
    12241 caaggacggg cactgtaggt ctggtgggca ggttcctggc tacccaggcc acggcgtcgg
    12301 cgaagtcttc gcgaactaat cggaatttca aatcggtgag accaacggtt gtcgtcgcca
    12361 cgtccatagc gtcccttcga tacacaccac aataaggtct aaccagcggt ttctcggtgt
    12421 cagaccacgc gtctccccga tgcgtgtcga cgaccgtaac ggctgtcgct gaaccaccgt
    12481 agatctttcg cgctcaactt gaaagctaat cgatgggggt gacattgggc gtcgcaaagc
    12541 cgttttgcgc gcagtgaatc gatgtgtccc caactggctt cttcaaagaa gaacctacgg
    12601 agatatagaa gcaacagtat tagggcttgt ggacattggg gataacccac tgtcggccca
    12661 gcgtggcacg cgtgccgggg tgtgagtgaa ctgtggatag cctgctgttg cagtcgggtg
    12721 gagtgtggac gccggtcgtg ctgtgaacga agggctgggt aatgcgacgg ttggccgcaa
    12781 cttgtccaca ccgcgatcca caccgcggtt gtgtgacaag tgataccgac ggtgccaaag
    12841 tttttagaag aatttttcgg cggaacgcgc cgagtcgggt cgacgaagag cggtcagcgc
    12901 ttggagcgct ggcggattcg tgtagtgagt tccttgacgt gatcgaacac ctcacggcgt
    12961 tcggccatct cgccgcggat cttcttctcg gcgtacatca ccgtggtgtg gtcgcggccg
    13021 aaggcctggc cgatcttcgg caacgacaaa tcggtgagtt cgcggcacag atacatggcg
    13081 atctggcggg actgcgcgag cgctcgggtc ttacccggtc cgcgcagttc ctccacggtg
    13141 gtttcgaagt actcggcggt ggcggccatg atcgcggccg tgctgatctg catggtggtc
    13201 gcgtcggcga tcaggtcgcg cagcacgatc tcggcgagtg ccttgtcgat cggtgtcttg
    13261 ttcagcgacg cgaatgccgt gacgcgaatc agcgcccctt cgagttcgcg gatgttgcgt
    13321 tcgatgctgc tggcgatgag ctcgagcacg tcgcccggta catcgaggcg gtccatctgt
    13381 gccttcttgc gcagaatcgc aatgcgggtc tcgagttcgg gtggctgaac gtcggtgatc
    13441 agtccccact cgaaccgggt gcgcagtcga tcttcgaggg tcgccagttg cttgggcggc
    13501 cgatcagagg agatgacgat ctgcttgttc gagttgtgca gcgtgttgaa ggtgtggaag
    13561 aactcctctt ggatgccttc cttgccctcg atgaactgaa tgtcgtcgac caggagcaca
    13621 tcgatgtcgc ggtagctgcg tttgaacgag gctttgcggt cgtcgcgcag cgagttgatg
    13681 aagtcgttgg tgaactcttc ggtggagacg tatttgacgc gcatgcccgg aaacagccgt
    13741 tgggcgtagt tgccggccgc gtgcaaaagg tgggtcttcc ctaggccgga ttcgccccag
    13801 atgaatagcg ggttgtatgc ccgcgcgggc gcctcagcaa tcgcgagtgt cgcggcgtga
    13861 gcgaacctgt tggaggctcc gatgacgaag gtgtcgaacg tgtagcggcg gttgagattg
    13921 accgcggtgg gctcgccgga cggggtgttt tgcagccggc tcgagaagta ggtcggccag
    13981 ctttcctcgg cgctcgcgag ggcctcggag tcttcatcga cttcgtcggg ttccgggctg
    14041 acggcggcgg gggtgaaccc gttgaggctc tcctcggagg cgtcggaagt cggggcggcg
    14101 atgcggacgc cgagctcgac acgcttgccg agctgacggc tgagtgcggt gatgatcggc
    14161 tcgcgcaggt ggcgttcgat ctcgttttgc acaaacggtg tcggcaccga cagcagggca
    14221 aatccctcgg taatgacgag cggctgaacc agcttcaacc aggccctttg ctggggggtc
    14281 agggtaggga ggctggaatc cccgttatga atttcgccgg tgtcggactc accgttgagt
    14341 tcggcaacca cgttgttcca gacggcgacg aagggtgggt cggggtcagc tgtcaatggc
    14401 tagtaccccc ttgaggccga tctagagacg accaaaggaa cgatgacgaa ctgtccacat
    14461 agttatccac aggttgtgga gacaggacag tcgccgccgc tctgttgtct accggcgagg
    14521 acgccgcggg ccggggctcg tcacagtggt gtggcgttct gtgggctcgc ggctgtgtgg
    14581 gcatccccgc ttcgttgtct gtcgccgttt cgttatcgaa tcggcattgg cagaagctaa
    14641 cagttttctc gtcgggtgcc aaccgttctg caacattctt ggggggagat ttttgcccgg
    14701 cggcggcacg ggaccgatgt ggcggttgtg aagctttgcg cgccgatcgg acgaggtttg
    14761 acccaagtaa atctcatcag taccctcgaa cagtcgcccg cacgtggcga tacggctgcg
    14821 acccaggagt ttctggagac cgcgccggct agcgagacgg acgagcttac caacggcaat
    14881 cgcgtcccca ccgtatcgat tcatcggagt gggttgcgga tgccagacgc aacaaggagt
    14941 gaacagccgt ggccaagggc aagcggacct tccagcccaa caatcgtcgt cgggcgcggg
    15001 tgcacgggtt ccggctgcgg atgcgcaccc gggccggccg ggcgatcgtg tcgaaccggc
    15061 gcggcaaggg tcgccgcaag ctcactgcgt gattcgacgc gggatctagc gcggtgcttc
    15121 cggctcgata ccggatgact cggtcgacgg agttcggcgc gaccgtcagt catggggtgc
    15181 gggcggcgca gccagatctc gtggtgcatg ccttgcggtc acccggcggc ggcgacggac
    15241 ctcgcatcgg attggtcgtc gcgaaatctg tgggtagcgc tgtgcagcgg catcgggtag
    15301 cgcgtcggct gcgacacgtc gcgcgcacgg tgatcgacga gttggagccg gcggaccggg
    15361 tggttatccg cgcgctgccg agcagccggc acgccatctc agcccgactg gagcaggaac
    15421 tgcggaccgc gatacgccga acccgaccgc gaagtgggtc gagccgatga gtgcggctca
    15481 ggtaggacgc aacggctcac ccgttgtgcg gggagtggtc ttcgtcattg agctctaccg
    15541 ccacatgatc tcgcctatgc ggttacccac gtgccgcttc accccgacct gtagccagta
    15601 cgcggtcgac gcgctgaccg agtacggctt ggtgcgtggc ggctggctgg ccctgctgcg
    15661 actgctgaag tgtgggccgt ggcatcgggg aggatgggat ccgatacccg aacgccgcag
    15721 cgccgtgcct gatgaccgtg agtgcggacg tgccgctgac gatctggaaa gcgagattcg
    15781 tgtttaactg gttcagcctg gacgtcatct attacccggt gtcggcgatc atgtgggttt
    15841 ggtacaaggc gttcgcgttt gtcctgggcc cggcgaactt cttcgcgtgg ggactgtcgg
    15901 tgatgttcct cgtcttcacg ctgcgcgcga tcctctacaa accgttcgtt cggcagatcc
    15961 gcaccacgcg gcagatgcag gaactgcagc cgcagatcaa ggcgctgcag aagaagtacg
    16021 gcaaggaccg ccaacgcatg gcgctggaaa tgcagaagct ccagaaggag cacggattca
    16081 atccgatcct cggctgcctg ccaatgctcg cgcag
//
LOCUS       DASRJA010000602         2802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2956116,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000602 DASRJA010000000
VERSION     DASRJA010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2956116"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFC01_26780"
     CDS             <1..329
                     /locus_tag="VFC01_26780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HZN83254.1"
                     /translation="LYEFCYDASPIGAAHTTEIPIVFGTLDSPVGSALYGSAPDADAV
                     SREMSAAWRAFAATGDPGWPAYEAVQQHTRVFDVSSTTTRYPEQASQRIWVDYPFDPF
                     GVNATR"
     gene            complement(290..1078)
                     /locus_tag="VFC01_26785"
     CDS             complement(290..1078)
                     /locus_tag="VFC01_26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014984388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN83255.1"
                     /translation="MTSLSGKTALVTGASRGIGRAIAQRLAADGAEVAVHYGSNEAAA
                     ADTVAAIKAAGGQAFPVRAELGVDGDVDTLFAALEQGLDGRPLDILVNNAAIATYEAT
                     IEATPEQFEREFAINVRAPFFVTQRALPLLRDGGRIINITSGVTWFATPQIVYSMTKG
                     ALNVLGRSLANIVGERGITVNNVSPGVTDTDMNSWLHESEEAVSGIAGITALDRVGAS
                     ADIADAVAFFASDDSRWVTGQTVEVNGGLWLGQRVAFTPNGSNG"
     gene            complement(1107..1886)
                     /locus_tag="VFC01_26790"
     CDS             complement(1107..1886)
                     /locus_tag="VFC01_26790"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZN83256.1"
                     /translation="MTEPLVLVADHGAVRLLTLNRPEARNALSRNLIRATYTALTEAD
                     ANESVRAVVLTGADPAFCAGVDLKEAQRDGLAYFEEFRSQSCIAAVAKMRTPIVGAIN
                     GACFTGGLEMALGCDFLVASERAVFADTHARVGILPGGGMTARLPQLVGLAMARRLSM
                     TGEVVDAARAEHIGLVTEVVPHEQLLDRALELAGQIAEVPGATMSGLKEIYTTGAAAV
                     VDPALAAEEKIAFAQHRNFEGLGDQFRAVSERNKAQIDHPE"
     gene            complement(1883..>2802)
                     /gene="nuoN"
                     /locus_tag="VFC01_26795"
     CDS             complement(1883..>2802)
                     /gene="nuoN"
                     /locus_tag="VFC01_26795"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HZN83257.1"
                     /translation="LYGYAGTLSLSGIADAIHANTGKASLALIGVGLLLVGVLFKVGA
                     VPFHSWIPDVYQGVPTPITAFMAAATKIAAFGALLRIFYVAVPEIRADWRPVLWAIAI
                     LTMVVGTVTAVTQSDVKRMLAYSAVAHTGFILTGVIALNESGLSSTLFYLFAYGFSTV
                     GAFAVVGLVRNPDGEEDTALERWAGLGRRYPVVGIVFSLFLLAFAGIPLTSGFVSKFA
                     VFKAAGEGGAIPLVVIGVIASAVAAYFYVRVIVLMFFTEPPDDAPAVVVPSVLSTAVV
                     TITATVTFALGALPQPLLDLANTANQFLP"
BASE COUNT          498 a          943 c          917 g          444 t
ORIGIN      
        1 atctctacga attctgttat gacgcctcgc cgatcggagc ggcacacacc accgagatcc
       61 cgattgtgtt cggcacgctg gattcacccg tcggctcagc gctgtatggg tcggcgccgg
      121 acgccgacgc ggtgtcgcgt gaaatgagcg cagcctggcg ggcgttcgct gccaccggtg
      181 atcccggctg gcccgcctac gaggccgtgc agcaacacac ccgggtcttt gacgtcagct
      241 caacgacaac gcgctacccc gagcaagcct cgcaacgcat ttgggtggac tacccgttcg
      301 acccgttcgg agtgaatgcg acgcgctgac cgagccagag gccgccgttg acctcgacgg
      361 tttgaccggt gacccaccgg ctgtcgtcgg aagcgaagaa cgccacggcg tcggcgatgt
      421 cggccgaggc gccgacacgg tccagcgcgg tgatgcccgc gatgcccgac accgcctctt
      481 ccgactcgtg cagccacgaa ttcatatcag tgtcggtgac tccgggggag acgttgttga
      541 cggtaatccc tcgctcacca acgatattgg cgagcgatcg gcccagcacg ttgagcgcgc
      601 ccttggtcat cgaatacacg atctgcggcg tcgcgaacca ggtgacgccg gaagtgatgt
      661 tgatgatgcg gccgccgtcg cgcagcagcg gcaacgcgcg ctgggtgacg aagaacggcg
      721 cccgcacatt gatggcgaac tcccgctcga actgttctgg cgtggcctcg atcgtcgcct
      781 cataggtggc gatggcggcg ttgttcacca ggatgtcgag cggacgcccg tcgagaccct
      841 gttccagtgc tgcgaagagc gtgtcgacgt cgccgtcgac gccaagctcg gcacgcaccg
      901 ggaaggcctg tccacccgct gccttgatcg cggcgacggt gtcggccgcc gcggcttcgt
      961 tgcttccgta atgcacggcg acctccgcgc cgtcagcggc gagtcgttgg gcgatggcgc
     1021 ggccgattcc ccgcgatgct ccggtcacca gtgcggtctt gcccgataga gaagtcatgt
     1081 gttgggcaac cggctgtcga ggcgagctat tccggatggt cgatttgcgc cttgttgcgc
     1141 tcggagaccg cgcggaactg gtcgccaagg ccctcgaagt tccgatgctg cgcgaaggcg
     1201 atcttctcct ccgccgctaa cgcggggtcg acgaccgcag cggcgccggt cgtatagatc
     1261 tccttgagtc ccgacatcgt tgcgcccggc acctcggcga tctgaccggc cagctcgagt
     1321 gcccggtcaa gcagctgctc gtggggtacc acctcggtga ccaaaccgat gtgctccgca
     1381 cgcgccgcgt cgaccacctc gcccgtcatg gacagccgcc gtgccatcgc caggccgacg
     1441 agctggggga gccgtgcggt catgccgccg cccggcagga tgccgacgcg ggcgtgcgtg
     1501 tcggcgaaca ccgcgcgctc cgacgccacg aggaagtcgc agccgagcgc catttccagt
     1561 ccaccggtga agcacgcgcc gttgatcgcg ccgacgatcg gcgtgcgcat cttcgcgacc
     1621 gcggcgatgc agctctgaga ccgaaactcc tcgaagtacg caagcccgtc gcgctgcgcc
     1681 tccttgagat ccacgcctgc gcagaatgcc gggtcggcgc ccgtcaacac caccgcccgc
     1741 acggactcgt tggcgtcggc ctcggtcagt gccgtgtacg tcgcccggat caggttgcgg
     1801 ctcaacgcat tcctggcctc cggccggttc agggttagca acctgacagc gccgtggtcg
     1861 gctacgagga cgagcggttc agtcatggca agaactggtt ggcggtgttc gccaggtcca
     1921 gcagaggctg cggcagggca ccgagggcga acgtcacggt cgcggtgatc gtgacgaccg
     1981 cggtgctcaa cacgctggga accaccacgg cgggtgcgtc gtcgggcggc tcggtgaaga
     2041 acatcaatac gatcacccgc acgtagaagt acgcggctac cgcgctggca atgacgccga
     2101 tgacgaccaa cggaatcgcg ccgccctcgc ccgcggcctt gaacaccgcg aacttgctga
     2161 cgaacccgct tgtcagcggg ataccggcga aggcgagcag gaacaacgaa aacacaatgc
     2221 cgacaacggg ataccgtcga cccagaccgg cccaacgttc taacgccgtg tcctcctcgc
     2281 cgtcgggatt gcgcaccaga ccgaccaccg cgaaggcgcc gacggtgctg aacccgtagg
     2341 cgaacagata gaacagcgtc gacgacaaac ccgactcgtt cagcgcgatc acgccggtca
     2401 ggatgaaccc ggtgtgggcg accgcggaat aggcgagcat ccgcttgacg tcggattggg
     2461 tgaccgcggt gaccgtgccg accaccatgg tcaggatcgc gatcgcccac agcaccggcc
     2521 gccagtcggc cctgatctca ggtaccgcga cgtagaagat gcgcagcagc gcgccgaacg
     2581 ccgcgatctt ggtggctgcg gccatgaacg cggtgatcgg cgtcggcacg ccttgataga
     2641 cgtcggggat ccacgagtgg aacggcaccg cgccgacttt gaacagcaca ccgacgagca
     2701 gtaggccgac cccgatgagc gccagcgacg ccttaccggt gttggcatgg atcgcgtcgg
     2761 cgattcccga caggctcagc gtgcccgcgt agccgtacag ca
//
LOCUS       DASRJA010000603         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2892207,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000603 DASRJA010000000
VERSION     DASRJA010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2892207"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VFC01_26800"
     CDS             complement(<1..1056)
                     /locus_tag="VFC01_26800"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZN83258.1"
                     /translation="MRSRSRKFWIGAAVLATAAAVVVPARVWVADGFVAGASGLGDPF
                     FPLGGNGGYDVTNYSLVLDYSPSTRHLDGTATITATATENLSRFDLDLRGFDVSAVMV
                     NDRAASFTRDGQELVITPRTGLRSGRSFTVVVSYGGVPVVITDPDESIEGWIPTDDGA
                     FVVGEPQGSPGWYPANDNPRDKATFDIKVTVPDGLTAMANGVLVSQTSSGGKATFVWR
                     ENSPMAPYLATATLGRFDFTQYQLASGLPVYIAVDPTLSTASVLNKLPDIVEFYSSIY
                     GPYPFDAVGAIVDDAKNVGYSLETQTKPVFDRVPDKATLAHELSHMWFGDSVTLTLWP
                     DMWLHEGFATWSEWIWSE"
     gene            1416..2204
                     /locus_tag="VFC01_26805"
     CDS             1416..2204
                     /locus_tag="VFC01_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006553113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HZN83259.1"
                     /translation="MGHRQVHGEIAPPVFLSVAGHPVRWRLLSELARSDRQVHELTAL
                     VGQPQNLVSYHLGRLREANLVVARRSTADARDIYYSVDLARCGRLLTAAGGALHPALL
                     LAQPTASVRQRGRARVRVLFLCTGNSSRSQMAEALLRQVADGALHVCSAGSNPKQVHR
                     FAVAVMVEHGIDLTKAHAKHLDKFIGQRFDYVITLCDRVREVCPEFPGDPQPIHWSMP
                     DPAEEPDGYPAFQRVASELAGRIGFLMHHIALHPITPGLIVEES"
     gene            2201..2593
                     /locus_tag="VFC01_26810"
     CDS             2201..2593
                     /locus_tag="VFC01_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN83260.1"
                     /translation="MSIVSDGTVNVRYMVDDVQAAVDFYTTHFGFTVNTMALPAFADV
                     TRGNLRLLLAGPRSSAGRAMPDGRKPEPGGWNRIHFIVDDIAAEVDRLRAGGVSFRND
                     IITGPGGRQILLDDPSGNPVELFQPAGT"
BASE COUNT          508 a          927 c          873 g          503 t
ORIGIN      
        1 ctcgctccag atccactccg accacgtcgc gaaaccctcg tgcagccaca tgtccggcca
       61 gagcgtcagc gtgacggagt cgccgaacca catgtgggac agttcatgcg ccagcgttgc
      121 cttgtccggc acccggtcaa acaccggttt ggtctgtgtc tccagcgagt acccgacatt
      181 cttcgcgtca tcgacgatcg cgccgaccgc atcgaacgga tagggcccat agatcgaact
      241 gtagaactcg acaatgtccg gcagcttatt caacaccgac gcggtcgaga gcgtggggtc
      301 gacggcgatg tagaccggta gtccgctggc gagttggtat tgcgtgaagt caaatcgacc
      361 gagcgtcgcc gttgcgagat acggggccat tggcgagttc tcccgccaga cgaatgtggc
      421 cttgccgcca ctggacgtct gcgacacgag tacgccattg gccatcgctg tcaaaccatc
      481 cggaacggtg actttgatgt caaaggtcgc cttgtcccgc ggattgtcgt tagcggggta
      541 ccacccaggg gatccctgcg gctcaccgac cacgaacgcc ccgtcgtccg tcgggatcca
      601 gccctcgatc gattcatccg gatcagtgat gacgaccgga acgccgccgt acgacaccac
      661 gacggtgaac gaccggcccg accggagtcc cgtccgagga gtgatgacga gctcctgacc
      721 gtctcgcgtg aaagacgcgg cgcgatcatt caccatgacg gccgagacgt cgaacccccg
      781 cagatcgaga tcgaaccttg aaaggttttc cgttgccgtc gcagtgatcg tggcagtccc
      841 gtccagatga cgtgtcgagg gactgtagtc cagaacgagc gagtagttcg tgacgtcgta
      901 gccgccgtta cccccgagcg ggaagaacgg atcgccaagc ccggacgccc ctgcaacgaa
      961 tccgtccgct acccagaccc gagcgggaac gacaacggca gcggccgtcg ccagcacagc
     1021 ggcgccaatc cagaactttc tactccgaga cctcatcggg ttcatcgcca tccccctcgg
     1081 ttggcttccc ccgtacaggc cgtccactcc aaatcggtta ctggaacaag cagacggtac
     1141 cgccgtggcg ggctgaatgc atcgcataag gtgcatgcag tgtcaagctg ccgatcaggg
     1201 ccccagcctg tcgcttcagc cgaacgacag atttcaggat cgcccagcgg ccagcagggt
     1261 tcgtcgggat ccattgtgga tagccagact caggtttggt cgctctgcgc gttccgagac
     1321 atctaccggc cgcaggaggc cctgttcgct cttttcgtcg gcgacgcccc cgtcacacgt
     1381 gttgcatcaa atcagtttga tgtattctcc ggcttgtggg gcaccgacag gttcacggtg
     1441 aaatcgcgcc gccggtattc ctgtccgtgg ccgggcatcc cgttcggtgg cggctgctga
     1501 gcgagctagc tcgcagcgac cggcaggtgc acgagttgac cgccctggtg ggtcagccgc
     1561 aaaacctggt ctcttaccat ctcgggcggc tgcgtgaggc gaacctggtc gtggcccggc
     1621 gcagtaccgc tgacgcccgc gacatctact acagcgtcga cctggcccgg tgcgggcggc
     1681 tgctgaccgc cgctggcggg gcgctgcacc ccgcgctgct gctggcccaa cccaccgcgt
     1741 cggtgcggca gcggggccgg gcgcgggttc gggtgttgtt cctgtgtact ggcaacagct
     1801 cccgctcgca gatggcggaa gcgctgctgc ggcaagtcgc ggacggtgcc ctgcacgtgt
     1861 gcagtgcggg cagcaacccc aagcaggtgc accggtttgc ggtagctgtg atggtcgaac
     1921 acgggatcga cctgaccaag gcccacgcga agcacttgga caaattcatc gggcagcggt
     1981 tcgactacgt catcaccttg tgtgaccggg tccgggaggt ctgcccggag ttccctggtg
     2041 acccgcaacc gatccactgg agcatgcccg accccgccga ggagcccgac ggttacccgg
     2101 cgttccagcg tgtcgcgtcc gaactggctg gccgaatcgg gttcctcatg caccacatcg
     2161 ctttgcaccc catcacacca ggtttgatcg tggaggagtc atgagcatcg tgtccgacgg
     2221 caccgtcaat gtccgataca tggtcgacga cgtgcaggcc gccgtcgact tctacaccac
     2281 ccacttcggc ttcaccgtga acaccatggc gttgccggcg ttcgccgacg tcactcgtgg
     2341 caacctgcgg ctgctgctgg ccggaccgcg aagctcggcc ggccgcgcga tgcccgacgg
     2401 ccgcaaaccc gaacccgggg gttggaaccg gatccatttc atcgtcgacg acatcgccgc
     2461 cgaggtggat cggctgcgag cgggcggggt gagcttccgc aacgacatca tcaccggccc
     2521 aggcgggcgg cagattctgc tcgacgatcc ctctggcaac ccggtcgagc tgttccaacc
     2581 tgctgggacg tagatgggca aggaccccca gccaggatcc gacgagcctg ggggtcggat
     2641 cacaccatcg gcagttgatt gccgaggccg cgaactgcgg gtcgggttca gaacggccga
     2701 ttccgggccc ggtgcagacc agctcttgca acacacgcca ggaggggagt cgaggtttac
     2761 atacagagcc tcttatccgg ttcctcctga acgccgtccc tcgtccttgt t
//
LOCUS       DASRJA010000604        13084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1720862,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000604 DASRJA010000000
VERSION     DASRJA010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720862"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..556
                     /locus_tag="VFC01_26815"
     CDS             32..556
                     /locus_tag="VFC01_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZN83261.1"
                     /translation="MSLRIAALGLLAWEPASGYDLLKHFEKSMANVWPATQSQLYGEL
                     NKLADAGLIEVSDIGPRGRKEYRITEAGRAEIRRWVANPQDDPPFRSAGLLRVFLLDA
                     IPREQARAHMVAVAEHADAEVKRLEALRDSIGWGEDESFFYGRAALEYGLRMNAMEAD
                     WARWLVNAIDTREA"
     gene            650..1027
                     /locus_tag="VFC01_26820"
     CDS             650..1027
                     /locus_tag="VFC01_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83262.1"
                     /translation="MNTKHDHKKSAFAAIGAAIAAAAVPAALITGAGTAQAATTLNTA
                     SDALGVTVEITSTGSEGLCFYSAEPWFGWSTVKPLPVYKVPFYLQANQSHNLWFPGIQ
                     TGTTWKAKVECPVGGVDFFNPVY"
     gene            1171..1899
                     /locus_tag="VFC01_26825"
     CDS             1171..1899
                     /locus_tag="VFC01_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZN83263.1"
                     /translation="MTNPFTPPPGGFGFGFGPGDRRAFHQQRRQARREFRDQVRDHLR
                     EHDAGPGFGPGRGFGPGRGFEFGFDPRGGFGFGFGGPGGPRGRGRGGRGRGKRGDVRA
                     AILKLLAERPMHGYEMIQEIAERSQNLWRPSPGSVYPTLQLLVDEGLLVARESEGSKK
                     LFELTDDGRAAADKVETPPWEEITDGLKDEAPGHVNLRSAVGQLMGAVAQSAYATDEE
                     QQQRIVDILNNARREIYGILGESD"
     gene            1939..2814
                     /locus_tag="VFC01_26830"
     CDS             1939..2814
                     /locus_tag="VFC01_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZN83264.1"
                     /translation="MQTFPLGSFHVGRVGFGAMQLPGPGVFGPPRDHDQAIAVLRRAI
                     ELGINHIDTAQFYGPNVSNELIREALHPYPKDLALVSKVGGRRDDQGNWLPAQEPDHL
                     RSDIESNLRTLDTDQLAAVNLRVFGGDDASVPGTLDRELFDRQLSAMIKARDEGLIAG
                     IGLSSISVEHLQFALDRTEIVTVQNAYNLVDRTSQPVLEACVEHGVSFVPFFPLGSGF
                     TADNPVLGHPAVQREASKLGRTPAQIALAWTLSVAPNVLLIPGTSSVSHLEENTAVAD
                     IELDDETKQQLDAAA"
     gene            complement(2815..4332)
                     /gene="glpK"
                     /locus_tag="VFC01_26835"
     CDS             complement(2815..4332)
                     /gene="glpK"
                     /locus_tag="VFC01_26835"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HZN83265.1"
                     /translation="MAEFVAAVDQGTTSTRCMIFDHDGAEVGRHQLEHEQILPKAGWV
                     EHNPVEIWERTASVIMSALNKTKLSATDLAALGITNQRETALVWNRRTGRPYHNAIVW
                     QDTRTDRVASALDRDGRGDVIRRKAGLPPATYFSAGKVQWILENVDGVRADAEKGDAI
                     FGTPDTWVLWNLTGGPRGGVHVTDVTNASRTMLMNLETLDWDDELLSFFGIPREMLPE
                     IKPSSYSESYGITRDDGPVSGQVPITGILGDQQAAMVGQVCLDAGEAKNTYGTGNFLL
                     LNTGEKIVRSENGLLTTLCYQFGNAKPVYALEGSIAVTGSAVQWLRDQLGIISGAAQS
                     EALARQVDDNGGVYFVPAFSGLFAPYWRSDARGAIVGLSRFNTNAHLARATLESICYQ
                     SRDVVDAMEADSGVHLEVLKVDGGITANDLCMQIQADVLGVDVVKPVVAETTALGAGY
                     AAGLAVGFWEDPDDLRANWQEGRRWTPQWSEEQRAEGYAGWQKAVQRTLDWVDVD"
     gene            4370..5131
                     /locus_tag="VFC01_26840"
     CDS             4370..5131
                     /locus_tag="VFC01_26840"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN83266.1"
                     /translation="MVYMGSKTAGIDRMPRGGPDASCLDRLLQTDRPEYLDRDDVDDN
                     VKRSVIRALEWTGEFFGNTERFARIVLDEVAEVPDPKILELGSGHGGLSRKLLEWHPT
                     AELTVTDVETQSVKAIEASDLGDHARATVREMDATAIDVPDRHFDLAVFAFSFHHLPP
                     SLASRVFAEGTRAAAKLLIIDLPRPPSPLHILRLAAMLPLAPLVPFVHDGVISSLRCY
                     SPSALRALATHADPEIHVELRGGLMSPQVVVASRG"
     gene            complement(5133..6272)
                     /locus_tag="VFC01_26845"
     CDS             complement(5133..6272)
                     /locus_tag="VFC01_26845"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003925243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZN83267.1"
                     /translation="MRPALNDVDQGGLEQVSTVDRVASLTGVRTVAALLVMGTHAAYG
                     TGKSTHGYVGLIYARMDIGVAVFFVLSGYLLFGPWVRAAATGAEPPSVRRYARNRLRR
                     LMPAYLVTVLAAYAIYEFREPGPNPGHTWAGLVRHLTLTQIYSGDYLSLYLHQGLTQM
                     WSLAVEMAFYAVLPPTAYLLLAVLCNRRWRPERLLAGLAVVGSVTPLWLIMMHTTDWL
                     PKGAGLWLPHYLVWFVGGMALAVLQVIGFRCYAAAVIPVAVVAYLVVATPIAGDAVSP
                     SPDLGQDLSRSLFYAAIAALVVAPPALGDRGVYTRLLGSPPMVWLGEISYEIFLVHVI
                     VMEIAMASVLRWPVYTGSVLGLFAVTLALSIPVAWLLHRLTRPRV"
     gene            6341..7819
                     /locus_tag="VFC01_26850"
     CDS             6341..7819
                     /locus_tag="VFC01_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HZN83268.1"
                     /translation="MGEEVKQTDFSRAHRREYRRKVQLCLDVFETMLAQSSFEFDRPL
                     TGMEIECNLVDGEYQPAMSNQEVLASIADPAYQTELGAYNIEFNVPPRRLPGRAALEL
                     EDDVRASLNAAEEKANSNGAHIVMIGILPTLMPEHLSGGWMSESTRYQALNDSIFTAR
                     GEDILIDIAGPERLSLQLASIAPESACTSMQLHLQVSPAEFARNWNAAQVLAGPQLAL
                     GANSPYFFGHQLWPETRIELFAQATDTRPDELKAQGVRPRVWFGERWITSIFDLFEEN
                     VRYFPSLLPELSDEDPVAALADGRTPKLAELRLHNGTIWRWNRPVYDVVEGRPHLRVE
                     NRVLPAGPTVVDMLASAAFYYGMLRTLSEEDRPLWTKLSFAAAQHNFLAAAQHGMEAR
                     LYWPALGEVTPDELVLRKLLPMAEEGLRRWEVASEVRDRYLGVIEGRAKTGRNGATWQ
                     VATVQALQERGMTRPKALAEMLRLYCERMHSNEPVHTWDGPD"
     gene            7830..8510
                     /locus_tag="VFC01_26855"
     CDS             7830..8510
                     /locus_tag="VFC01_26855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN83269.1"
                     /translation="MASDEVMNWDDTYREQGPFEGPPPWNIGEPQPELAALHRDGKFR
                     SDVLDAGCGHAELSLALAADGLTVVGIDLSPTAIAAATSAAQERGLTNASFVQADITN
                     FTGYDDRFNTVIDSTLFHSLPVEGRDGYLRSVHQAAAPGARYYILVFAKGAFPAELET
                     KPNEVDEHELRDAVSKHWEIDEIRPAAIHANALVIPDAPFEMPAHESDEKGRIKFPAF
                     LLTAHKAG"
     gene            complement(8511..9629)
                     /gene="egtE"
                     /locus_tag="VFC01_26860"
     CDS             complement(8511..9629)
                     /gene="egtE"
                     /locus_tag="VFC01_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis PLP-dependent enzyme
                     EgtE"
                     /protein_id="HZN83270.1"
                     /translation="MSLAEQWRAARPKVAGLHLDSGACSRQSFAVIDAATQHARHEAE
                     VGGYVAAAAAAPVLAAGRAAVGALTGLTDVVFTTGSNNALDILLSSWPGERTVACLPG
                     EYGPNLAVMAANGFQISALPADGLGRLALDDAARQLANDPPALVHLTPLASHRGIVQP
                     LAELADVCRDLGVPLVVDAAQALGHLDCAVGAAAIYSSSRKWIAGPRGVGALAIHPEL
                     AQRLRPRLPPPDWDLPIPVMQRLEMGEANVAARVGYSVALGEHLAAGPGQIRDRLAQI
                     GRMTRAALAGIKGWQVVEPLDEPTAITTLAPVDGADPERVRAWLIAERGIVTTYAELQ
                     RAPFEMTAPVLRASPHVDTTAQDLDQFAAALAAATAQV"
     gene            complement(9626..10591)
                     /gene="egtD"
                     /locus_tag="VFC01_26865"
     CDS             complement(9626..10591)
                     /gene="egtD"
                     /locus_tag="VFC01_26865"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HZN83271.1"
                     /translation="MTFSLSNYLAADSAAHALRRDVRDGLTQTPKTLPPKWFYDAVGS
                     DLFDQITRLPEYYPTRAEAQILRSRSADIAAASGADTLVELGSGTSEKTRMLLDALRD
                     SGSLRRFIPFDVDSGVLNAAGSAIQREYPGIEIDAVCGDFEEHLGKIPRVGRRLVVFL
                     GSTIGNLTPGPRADFLSALSDTLQPGDSLLLGTDLVKDAGRLVSAYNDGAGVTAQFNR
                     NVLAVVNRELDANFDLAAFGHVAKWNAGEERIEMWLRASSPQRVHIAALSLTVEFATG
                     EEMLTEVSCKFRSDGVADELAAAGLRRTHWWTDPAGDFGLSLSTK"
     gene            complement(10591..11280)
                     /gene="egtC"
                     /locus_tag="VFC01_26870"
     CDS             complement(10591..11280)
                     /gene="egtC"
                     /locus_tag="VFC01_26870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtC"
                     /protein_id="HZN83272.1"
                     /translation="MCRHLGWLGEPRSIASLVLEPSSGLLVQSYAPRRQKHGLMNADG
                     WGVGFFDNGVARRWRSAAPLWGDASFASVAPALSSNCVVAAVRSASIGMPIEPSASAP
                     FTDGVWLLSHNGLVDRAVLPLSSKAESTVDSALLAALIFERGLDALGDTIVEVAAADP
                     NARLNILAANGSQLRATTWGDTLSVLRQDDGVVLASEPYDDNPDWQEIPDRHLVVVEG
                     SRVELIPMKGS"
     gene            complement(11280..12554)
                     /gene="egtB"
                     /locus_tag="VFC01_26875"
     CDS             complement(11280..12554)
                     /gene="egtB"
                     /locus_tag="VFC01_26875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HZN83273.1"
                     /translation="MTAREKLARELTTARDRTLRLVDFDDAELGRQYDPLMSPLVWDL
                     AHIGWQEELWLLRGNNPDRPGLLEPQVERCYDAFVNSRASRVNLPLLPPSDARMYCST
                     VRNKVFDALDNLNNDDTGFTFGLVISHENQHDETMLQALNLRSGPPLLDRGSVLPSGR
                     AGVAGESVLVAGGEFVLGVDAVSEPHSLDNERDAHVVDLPAFRIGRVPVTNGEWRHFI
                     DDGGYQQQRWWSERGWTHRQEAGLTAPQFWNLDGTRTRFGHIEEIPADEPVQHVTYFE
                     AEAYAAWAGARLPTEVEWEKAAAWDPVAGKRRRFPWGASEPTEHLVNLGGDALRPAPV
                     GAYPAGASAYDAEQMLGDVWEWTSSPLRPWPGFTPMLYEQYSQPFFDGDYKVLRGGSW
                     AVASSILRPSFRNWDHPIRRQIFSGVRLAWDA"
     gene            complement(12551..>13084)
                     /locus_tag="VFC01_26880"
     CDS             complement(12551..>13084)
                     /locus_tag="VFC01_26880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HZN83274.1"
                     /translation="PVMLVHPTPEMDAVPITDWVPFADWADGRVLLGDRRPTQADLEY
                     HLTTLFPPVRPRRWLEIRYLDSLPDAVWPAVVFTLVTLLDDPVAADIAAEATEAVATV
                     WDRAAQLGLGDRRLHEAATRCVQTAAERAPAELEESMGQLMRSVEKGRCPADDFSDRA
                     VKFGIDTAITQLAQGEL"
BASE COUNT         2306 a         4414 c         4286 g         2078 t
ORIGIN      
        1 tgacatgtca atagtgaagt acgatcagcg cgtgagtttg cgaattgcgg cattgggcct
       61 tctggcatgg gagcccgcca gcggatacga cctgctcaaa cacttcgaga aatcgatggc
      121 caacgtctgg ccggcgacac agagccagtt gtacggcgaa ctgaacaagc tcgccgacgc
      181 gggcctgatc gaggtgtcag acatcggtcc gcgtggccgc aaggagtacc gcatcaccga
      241 ggccggacgc gcagaaattc gtcgctgggt ggcgaacccg caggacgatc cgccttttcg
      301 cagcgcgggc ctactccggg tcttcctact cgatgcgatt ccacgcgagc aggcgcgagc
      361 acacatggtt gcggtggcag aacatgccga cgccgaggtc aaacgcttgg aagcgttgcg
      421 tgactcgatc ggctggggcg aagacgaaag cttcttctac ggccgcgccg cgctggagta
      481 cgggttgcgg atgaacgcga tggaggcgga ctgggcgcgc tggctggtca acgccatcga
      541 cacgcgcgag gcgtgatcgt cgtcgaaaaa gtgtcagaca actggggcgc gatcgctcct
      601 acgtgtgaga gcagcccaaa ggggcggcaa ccagacgaag gagcaccaca tgaacaccaa
      661 gcacgaccac aagaaatccg ccttcgcggc gatcggcgcg gcaatcgctg cggcggccgt
      721 gcccgcggct ctgatcaccg gcgcgggcac cgcgcaggcg gcgacgacgc tcaacacggc
      781 tagtgacgcg ttgggcgtta cggtcgaaat cacctccacg gggagcgaag gcctgtgctt
      841 ctacagtgcc gagccgtggt tcgggtggtc gaccgtgaag cctttgccgg tgtacaaggt
      901 gccgttctat ctgcaggcga atcaatccca caatctgtgg tttcccggca tccagaccgg
      961 caccacctgg aaggcgaagg tcgaatgccc cgtcggtggc gttgatttct tcaaccccgt
     1021 ctactgagca tcgccgtctc ccggtgtggt cctcgtggcg tgtgttgcga ggaccgcacc
     1081 attccgacgt tgcatcgcga tagttttcga tatatcgtca acgtatcgga aacgcgtacg
     1141 gcgtttcccc acacacgtaa ggaagcagcc atgaccaacc cattcacccc acctcccggc
     1201 ggattcggat tcggcttcgg ccctggcgat cgacgggctt ttcatcagca gcgccgccag
     1261 gcccgccgcg agtttcgcga tcaggttcgt gaccatctgc gcgagcacga cgcaggcccc
     1321 ggctttggtc cgggccgcgg tttcggcccc ggccggggct tcgagttcgg attcgatccg
     1381 cgcggcggtt tcggcttcgg cttcggcggc ccaggcggtc cgcgtggccg tggccgcggc
     1441 gggcgaggac gcggtaagcg cggcgacgtc cgcgcggcga tcctcaagct gctggccgag
     1501 cggccgatgc atggctacga gatgatccag gagatcgccg agcgcagcca gaatctgtgg
     1561 cggcccagcc ccggctcggt ctacccgacg cttcagctgc ttgtcgacga gggcctgctg
     1621 gtcgcgcgcg aaagcgaagg cagcaaaaag cttttcgagc tcaccgatga cggccgcgcc
     1681 gccgccgaca aggtcgagac cccgccgtgg gaagagatca cggacggctt gaaagatgaa
     1741 gcgcctggac acgtgaacct gcgcagcgcc gtcggccagc tgatgggcgc tgtcgcgcaa
     1801 tcggcgtatg ccaccgacga agagcagcag cagcggatcg tcgacatcct caacaacgcc
     1861 cgccgcgaga tctacggcat cctcggcgaa tccgactaag gcggaataag cccggcggga
     1921 gtggtgtacg gaatctatat gcaaaccttc ccgctgggct cattccacgt cggccgcgtg
     1981 ggcttcggcg ccatgcaact ccccgggcca ggggtgttcg gcccgccgcg cgaccacgac
     2041 caggcgatcg ccgtgctgcg gcgcgcaatt gagttgggta tcaaccacat cgacaccgct
     2101 cagttctacg ggccgaacgt ctccaacgaa ctcatccgcg aggcgctgca cccgtacccg
     2161 aaggacctgg ccctggtgag caaggtcggc ggccgccgcg acgatcaagg caactggcta
     2221 cccgcccagg agcccgacca tctgcggtcg gacatcgagt cgaaccttcg cacgctggac
     2281 accgaccagc tcgccgccgt caacttgcgc gtgttcggag gcgacgacgc cagcgtgccg
     2341 gggacactcg accgcgaact gttcgaccgg cagctctccg cgatgatcaa ggcccgcgac
     2401 gaaggactga tcgccggcat cgggttgagc agcatctctg tcgagcatct gcagttcgcg
     2461 ctcgaccgca ccgagatcgt caccgtgcag aacgcgtaca acctcgtcga ccggacctcg
     2521 cagccggtgc tcgaagcgtg cgtcgaacac ggcgtctcgt tcgtgccgtt cttcccgctc
     2581 gggtcggggt tcactgccga caatcccgtg ctcggccacc cggccgtgca gcgcgaggcg
     2641 tcgaaactcg gccgcacgcc cgcccagatc gcgctggcgt ggacgctgtc ggtggcgccg
     2701 aacgtgttgc tgatccccgg gacgtcatcg gtcagccatc tcgaagagaa cacggccgtc
     2761 gccgacatcg aactcgacga cgagaccaag cagcagctcg acgcggccgc ctaatcagtc
     2821 aacgtcgacc cagtccagcg tgcgctgcac cgccttctgc caacccgcat agccttcggc
     2881 gcgctgctcc tctgaccatt gaggcgtcca gcgccgaccc tcttgccaat tggcgcgaag
     2941 gtcgtcggga tcctcccaga agccgaccgc caggcctgcc gcgtagccgg cgcccagggc
     3001 ggtggtttcg gcgacgacgg gcttgacaac gtcgacgccg agcacgtcgg cctgaatctg
     3061 catgcacaga tcgttggccg tgatgccgcc gtcgaccttc aaaacttcaa ggtgcacacc
     3121 agaatcagcc tccatggcgt cgacgacgtc gcggctctgg tagcagatcg actccagcgt
     3181 cgcgcgcgcc aagtgcgcat tggtgttgaa ccgcgacagc ccgacgatcg ccccgcgggc
     3241 atccgaccgc cagtacggcg caaacaaccc cgagaaggcg ggcacgaagt acacgccgcc
     3301 gttgtcgtca acttgccggg cgagcgcctc gctctgcgcc gcaccgctga tgatgcccaa
     3361 ctggtcgcgc agccattgca cggccgaacc ggtgaccgca atcgaaccct caagtgcgta
     3421 aacgggtttg gcgttaccga actgatagca cagcgtggtc agcaggccgt tctccgaccg
     3481 cacgatcttc tcgccggtgt tgagcagcag gaaatttcct gtgccataag tattttttgc
     3541 ttcgccggcg tccaggcaca cctgcccgac catcgccgcc tgctggtcgc cgagaatccc
     3601 ggtgatcggc acctgcccgg acaccggccc gtcgtcgcgg gtgattccgt acgactctga
     3661 atacgacgac ggtttgatct cgggcagcat ctcccgcgga atcccaaaga acgacagcag
     3721 ttcgtcgtcc cagtcgagcg tctcgagatt catcagcatg gtccggctgg cgttcgtcac
     3781 gtcggtgacg tgcacgccac cgcgcgggcc accggtgagg ttccacagaa cccaggtgtc
     3841 gggggtgccg aagatcgcgt cgcccttttc ggcgtcagca cgcacaccgt cgacgttctc
     3901 cagaatccac tgcaccttgc ccgcagagaa gtacgtggca ggcggcagcc cggccttgcg
     3961 cctgatgacg tcgccgcgac cgtcgcggtc cagcgccgag gcgacgcggt cggtgcgggt
     4021 gtcctgccag acgatcgcat tgtgatacgg ccgcccggtg cgccggttcc acaccagtgc
     4081 ggtttcgcgc tggttggtga tgccgagcgc ggccaggtcg gtggccgaca gtttggtctt
     4141 gttcagcgcc gacatgatga ccgatgcggt gcgttcccag atctcgacgg gattgtgctc
     4201 gacccagccg gccttcggca ggatctgttc gtgctcgagc tggtggcgcc ccacctcggc
     4261 gccgtcgtgg tcgaagatca tgcaccgcgt gctggtggtg ccctggtcga cggccgctac
     4321 gaactctgcc actggcactc cctcctgatg gcgactccaa tcgaccatca tggtctacat
     4381 gggttcgaag actgccggca ttgaccgcat gccccgcggc gggcccgatg cctcctgcct
     4441 cgaccggctg ttgcagaccg atcgaccgga atatctcgat cgcgacgacg tcgacgacaa
     4501 cgtgaagcgc agtgtgattc gggcgctgga gtggacgggc gaattcttcg gcaacaccga
     4561 gagattcgcc cgcatcgtgc tcgacgaggt ggccgaagtg cccgacccga agatcctgga
     4621 gctgggctcc ggtcacggcg ggctgtcacg caagctgctc gaatggcacc ccactgccga
     4681 actcacggtg accgacgtcg agacgcaatc agtcaaggcg atcgaggcca gcgacctcgg
     4741 cgaccacgcg cgggccacgg tgcgcgagat ggatgcgacc gcgatcgacg tacccgatag
     4801 gcacttcgac ctcgcggtgt tcgcgttctc ctttcaccac ttgccgccgt cactggcgtc
     4861 gcgcgtgttt gccgaaggca cccgcgcggc cgccaagctc ctgatcatcg acctgccacg
     4921 gccaccgtcg ccgctgcaca tcctgcggct ggcggcgatg ctgcccctgg cgccgcttgt
     4981 gccgttcgtg cacgacggcg tgatcagttc actgcgctgc tacagcccgt ctgcgctgcg
     5041 ggcactggcg acgcacgccg acccggagat acacgtcgaa ctacgcggcg gcctgatgag
     5101 tccgcaggtc gtggtggcca gccgcggcta gctcacaccc gaggccgggt cagccggtgc
     5161 agcagccacg ccaccgggat cgacagcgcc agcgtcacgg cgaacagccc gagcaccgag
     5221 ccggtgtaga ccggccagcg cagcaccgac gccatcgcga tctccatcac gatcacgtgc
     5281 acgaggaaga tctcgtacga gatctcgccg agccacacca tcggcggact gcccagcagg
     5341 cgggtataca ccccgcggtc acccagcgcc ggcggcgcca ccaccagcgc cgcgatcgcc
     5401 gcgtagaaca acgaccgcga caggtcctgg ccgagatcgg gcgacggcga tacggcgtcg
     5461 ccggcgatcg gtgtggccac caccagatac gcaaccactg ccacagggat gactgccgct
     5521 gcgtagcaac ggaatccgat gacctgaagc accgccaacg ccatcccgcc gacgaaccac
     5581 accagatagt gcggtagcca caacccggcg ccttttggca gccaatccgt cgtgtgcatc
     5641 atgatcaacc acaacggcgt caccgaaccc acgacggcga ggccggccag cagacgctcc
     5701 gggcgccagc gccggttaca caggaccgcc aacagcaggt acgccgtcgg cggcagcacc
     5761 gcgtagaacg ccatctccac cgccagactc cacatctgag tcaggccctg atgcagatag
     5821 agcgacagat agtcgccgct gtagatctgc gtcagggtca gatggcgcac cagacccgcc
     5881 caggtgtgac ccgggttcgg gccgggctcg cggaattcgt agatggcgta ggccgccagc
     5941 accgtgacga ggtacgcggg catgagccgc cggagccggt tgcgcgcata acgccgcaca
     6001 gatggtggct cggcgcccgt cgcggcggca cgcacccatg gcccgaacag caagtaaccc
     6061 gagagaacga agaacaccgc gacgccgatg tccatgcgcg cgtagatcag cccgacgtat
     6121 ccgtgcgttg acttgcccgt gccgtaggcc gcgtgggtac ccatgacgag cagcgccgca
     6181 acggtccgca caccggtgag tgatgcgacg cggtcgaccg tcgaaacctg ctcgagcccg
     6241 ccttggtcga cgtcgttcaa tgccggcctc atcgcgccag tatgggctcg ataccgcgat
     6301 ttccttgcgc ggtcgtcgac aacgctgaac catcggcgtt gtgggcgaag aggtgaagca
     6361 gaccgacttt tctcgcgcgc accgccgcga ataccggcgc aaggtgcagc tatgcctcga
     6421 cgtgttcgaa acgatgctcg cccaatccag tttcgaattc gaccgcccgc tgaccggcat
     6481 ggagatcgag tgcaatctcg tcgacggtga atatcagccg gcgatgagca atcaggaagt
     6541 gctggcgtcg atcgccgacc ccgcctacca aaccgaatta ggcgcgtaca acatcgaatt
     6601 caacgttcca ccgcggcggc tgcccggccg cgccgcgctg gagctcgagg acgatgtgcg
     6661 ggccagcctc aacgccgcgg aagagaaggc gaattccaac ggcgcgcaca tcgtgatgat
     6721 cggcatcctg cccacgctga tgcccgaaca tttgtccggt gggtggatga gcgagtcaac
     6781 ccgctaccag gcgctcaacg attcgatctt caccgctcgc ggtgaagaca tcctgatcga
     6841 catcgcgggc cccgagcggc tgagcttgca gctggcgtcg atcgcaccgg agtcggcgtg
     6901 taccagcatg caattgcacc tgcaggtctc ccccgccgag tttgcgcgca actggaacgc
     6961 cgcacaggtg cttgcgggtc cgcagctggc gctgggcgcc aactccccgt acttctttgg
     7021 tcaccagctg tggcccgaga cgcgcatcga gctgttcgcg caggcaaccg atacgcgccc
     7081 ggacgaactg aaggcgcagg gcgtccggcc aagggtctgg ttcggcgagc ggtggatcac
     7141 gtcgatcttc gacctgttcg aggagaacgt ccgctacttc ccgtcgctgc tgcccgaact
     7201 gtccgacgaa gatccggtcg ccgcgctcgc ggatggccgc accccgaagc tggccgaact
     7261 ccggctgcac aacggcacga tctggcggtg gaatcggccg gtctacgacg tcgtcgaggg
     7321 gcggccgcac ctgcgggtgg agaaccgtgt gctccccgcc gggcccaccg tcgtggacat
     7381 gttggccagc gcggcgttct actacggcat gctgcggacg ctgtccgaag aggaccggcc
     7441 gctgtggacg aaattgagtt tcgctgcggc acaacacaat ttccttgccg ccgcgcaaca
     7501 tggcatggag gcgcggctgt actggcccgc tctcggcgag gtcacacccg acgaattggt
     7561 actgcgcaag ctgctgccga tggccgagga aggtctgcgt cgctgggaag tggcgagcga
     7621 agtgcgcgac cggtacctcg gcgtgatcga ggggcgtgcc aagaccggac gcaacggcgc
     7681 cacctggcag gtggccaccg tgcaggcgtt gcaggaacgc ggcatgaccc ggcccaaagc
     7741 gctggctgag atgctgcggc tgtactgcga gcgaatgcac agcaacgagc ccgtccacac
     7801 ctgggacggc ccggactaag ttgggtgcca tggcatctga cgaggtgatg aattgggacg
     7861 acacataccg ggagcagggg ccgttcgaag gtccgccgcc gtggaacatc ggtgagccgc
     7921 agccagagct cgctgcgttg caccgcgacg ggaaattccg cagtgacgtg ctcgacgccg
     7981 gctgcggaca cgccgagctg tcccttgccc tggccgccga cggcctcaca gtggtcggca
     8041 tcgacctcag cccgaccgcc atcgccgcgg ccaccagtgc ggcacaggag cgcggcctga
     8101 cgaacgcgtc gttcgtccag gccgacatca ccaacttcac cggctacgac gaccgcttca
     8161 acaccgtcat cgacagcacc ttgtttcact cgttgccggt cgagggccgc gacggctatc
     8221 tgcgctccgt acaccaagcg gccgcgcccg gcgcccgcta ttacatcctc gtgttcgcca
     8281 agggcgcgtt tcccgccgag ctggagacca agcccaatga ggtcgacgag cacgagttgc
     8341 gcgacgcggt gtcgaagcac tgggagatcg acgagatccg gcccgcggca atccacgcca
     8401 atgccctcgt catccccgac gcaccattcg agatgccggc ccacgagtcc gacgagaagg
     8461 gccggatcaa gtttcccgcg tttttgctga cggcccacaa agcgggctag tcacacctga
     8521 gcggtggcgg cggcgagggc cgcggcgaac tggtcgaggt cctgcgcggt ggtgtcgacg
     8581 tgtggcgagg cccgcagcac cggcgcggtc atctcgaacg gggcgcgctg tagttcggcg
     8641 taggtggtga cgatgccgcg ctcggcgatc agccacgccc gcacccgttc cgggtcggcg
     8701 ccgtcgacgg gggccagcgt ggtgatcgcg gtcggctcgt caagtggttc gaccacctgc
     8761 cagcctttga tgccggccag cgcggcgcgg gtcatccggc cgatctgcgc gagccggtcg
     8821 cggatctgac cgggtcccgc ggcaaggtgc tcgcccaggg ccaccgaata acccacgcgc
     8881 gcagcgacat tcgcctcgcc catctccagg cgttgcatca ccggtatcgg taggtcccag
     8941 tccggcggcg gcagcctcgg cctcaggcgc tgcgcgagtt ccggatggat cgcgagcgcg
     9001 cccacgccac gcgggccggc gatccattta cgcgacgagc tgtagatcgc ggcggcccca
     9061 acggcgcagt cgaggtgtcc cagcgcctgc gcggcgtcga caaccaatgg cacgccgaga
     9121 tcgcgacaca cgtcggccag ttcggccagc ggctggacga tcccgcgatg gctggccagc
     9181 ggggtgaggt ggacgagcgc aggcgggtcg ttcgcaagct gccgggcagc gtcgtcgagg
     9241 gcgagccggc ctagaccgtc ggcgggcagt gcactgatct ggaatccgtt ggctgccatg
     9301 acggccaggt tgggaccgta ttcgccgggc agacaggcga cggtgcgttc gccgggccag
     9361 ctgcttagca ggatgtcgag tgcgttgttg gacccggtgg tgaagacgac gtcggtcagg
     9421 ccggtcaatg cgccgacggc cgcgcggccc gcggcgagca ccggcgcggc ggccgccgca
     9481 gcaacgtacc cgccgacttc tgcttcatgc ctggcgtgtt gggtggcggc gtcgatgacc
     9541 gcgaagctct gccgggagca cgcgccgctg tcgaggtgca ggccggcgac cttcgggcgg
     9601 gctgcccgcc actgctcggc gaggctcatt tggtcgacag ggacagcccg aagtcaccgg
     9661 cgggatcggt ccaccaatgc gtgcggcgca gaccggccgc agcgagttcg tccgccacgc
     9721 catcggaccg gaacttgcac gacacctcgg tgagcatctc ttcgccggtg gcgaactcga
     9781 cggtcaggct gagcgcggcg atgtgaacgc gttgcgggct cgaggcgcgc agccacatct
     9841 cgatgcgttc ctcgcctgcg ttccatttcg cgacatgccc aaaggcggcg aggtcgaagt
     9901 tcgcgtcgag ttcccggttg accaccgcca gcacgttgcg gttgaactgc gccgtaacgc
     9961 ccgcgccgtc gttgtaggca cttaccagcc ggccggcgtc cttgacgagg tcggtgccca
    10021 gtagcaggct gtcgccgggc tgcaacgtat ccgacagcgc ggacaagaaa tcggctcgcg
    10081 gaccgggtgt caagttgccg atcgtggagc ccaggaacac caccaagcgc cgacccacac
    10141 gcggaatctt gcccagatgt tcttcgaaat ctccacacac cgcatcgatt tcgatgccgg
    10201 ggtactcgcg ctgaatcgcc gacccggccg cgttgagcac cccggaatcc acgtcgaacg
    10261 ggatgaaccg acgcaaggat ccgctgtcac gcaaggcatc gagaagcatg cgggtcttct
    10321 ccgatgtccc gctcccgagc tcaacgaggg tgtcggcgcc ggacgccgcg gcgatgtccg
    10381 ccgagcgaga gcgcaggatc tgcgcctccg cgcgggtcgg gtagtactcg ggcagccggg
    10441 tgatctggtc gaacagatcg ctgccgaccg cgtcgtaaaa ccacttgggc ggcaacgtct
    10501 tcggcgtctg tgtcaggcca tcgcggacat ctcggcgcag tgcgtgcgcg gccgagtcgg
    10561 cggccagata gttggacagc gagaaagtca tcacgatcct ttcatcggga tcagctcgac
    10621 gcgagagccc tcgaccacaa ccagatggcg gtccgggatc tcctgccagt cggggttgtc
    10681 gtcgtacggt tcgctggcca gcaccacgcc gtcgtcctga cgcagcaccg acagggtgtc
    10741 gccccatgtg gtggcgcgca gttgagatcc gttggccgcc aagatgttca gccgagcgtt
    10801 ggggtcagcg gcggcgacct ccacgatggt gtcgccgagc gcatcgagtc cgcgctcgaa
    10861 gatcagcgcg gccagtagcg cgctgtcaac ggtggactcg gctttggacg acaacggcag
    10921 caccgcacga tcgacgagcc cgttgtggga cagcagccac acgccgtcgg tgaacggggc
    10981 cgacgccgac ggttcaatcg gcatgccgat gctggccgaa cgcaccgctg ccacaacaca
    11041 attactgctc aaggccggtg ccaccgaggc gaaggatgca tcaccccaca gcggtgccgc
    11101 gctacgccac cggcgtgcca cgccattgtc gaagaagccg acgccccagc cgtccgcgtt
    11161 catcagaccg tgcttctggc gccgcggcgc atatgactgg accagcagtc ccgaggacgg
    11221 ctccaacacc agcgatgcga tcgatcgcgg ttcgccgagc cagccgagat gtcggcacat
    11281 caggcgtccc aggccagccg aacacccgag aagatctgcc gccggatcgg gtggtcccag
    11341 ttgcggaagc tcggacgcag gatgctcgac gcgaccgccc acgagccgcc gcgcagcacc
    11401 ttgtagtcgc cgtcgaagaa cggttgtgag tactgctcat acagcatcgg ggtgaagccc
    11461 ggccacggcc gcagcggcga cgaggtccac tcccacacgt cgccgagcat ctgttcggcg
    11521 tcatacgccg aagcgcctgc cgggtaggcg ccgaccgggg cgggacgcaa cgcgtcgccg
    11581 ccgagattca ccaggtgttc cgtgggttct gaagcgcccc aagggaatcg gcgacgcttg
    11641 ccggccaccg ggtcccaggc ggcggccttt tcccattcga cctcggtggg cagccgcgcg
    11701 cctgcccacg ccgcgtacgc ctcggcctcg aagtaggtga cgtgctgcac gggttcgtcg
    11761 gcggggatct cctcgatgtg tccgaagcgg gtgcgggtgc cgtcaaggtt ccagaattgc
    11821 ggtgcggtca gcccggcctc ctgccgatga gtccagccgc gctcggacca ccagcgctgc
    11881 tgttggtagc cgccgtcgtc gatgaaatgc cgccactcgc cgttggtgac gggtacccgt
    11941 ccgatgcgga acgcgggcaa gtcgaccaca tgcgcgtcgc gttcgttgtc cagcgagtgc
    12001 ggttcgctca ccgcatcgac gccaaggacg aactcgccgc cagccaccaa caccgactct
    12061 ccggcgacac cggcgcgacc cgacggcaat acggatccgc gatccagcag gggcggccct
    12121 gaccgcagat tcagcgcctg cagcatggtc tcgtcgtgtt ggttctcatg gctgatcacc
    12181 aacccgaagg tgaaaccggt gtcgtcgttg ttcaggttgt cgagggcgtc gaacaccttg
    12241 ttgcgcaccg tgctgcagta catgcgggcg tccgagggcg gcaacagagg cagattcacc
    12301 cggctggcgc gggagttcac gaatgcgtcg tagcagcgct caacctgggg ctcgagcagt
    12361 cccggccggt cggggttgtt gccgcgcagc agccacagct cttcctgcca gccgatgtgc
    12421 gcgaggtccc acaccagcgg gctcatcaac gggtcgtatt ggcggcccag ttcggcgtcg
    12481 tcgaagtcga cgagccgtag cgtgcggtcc cttgcggtcg tcaactcacg cgcaagcttc
    12541 tcgcgtgccg tcacaactcc ccttgcgcca gttgggtgat cgcggtgtca atcccgaatt
    12601 tgactgcccg gtcggagaaa tcatcggccg gacatcgtcc cttctcgacc gaacgcatca
    12661 actggcccat cgattcctcg agttcggcgg gtgcgcgctc ggcggcggtc tgcacgcagc
    12721 gcgtggccgc ctcatgtagc cgtcggtcgc cgaggccgag ttgcgcggca cggtcccaaa
    12781 ccgtggctac ggcctcggtg gcctcggcag cgatgtcggc ggcgacggga tcgtcgagca
    12841 gcgtgacgag ggtgaacacc acggccggcc agaccgcatc gggcaggctg tcgaggtagc
    12901 ggatctccag ccaacgccgc ggacgaacag gcggaaacag agtggtcagg tggtactcga
    12961 ggtcggcctg cgtcggccta cggtcgccca gcagcacccg gccgtcggcc cagtccgcga
    13021 acggcaccca gtctgtaatc ggaaccgcat ccatctccgg cgtcgggtga accagcatca
    13081 ccgg
//
LOCUS       DASRJA010000605         4356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2285439,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000605 DASRJA010000000
VERSION     DASRJA010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2285439"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFC01_26885"
     CDS             complement(<1..496)
                     /locus_tag="VFC01_26885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZN83275.1"
                     /translation="MRLGLHALGIGSGATRSVIDAVAAAAEKSGFATLWSGEHVVMVD
                     RSASRYPYSDDGQIAVPATADWIDPLIGLSFAAAATSTIGIATGVLLLPEHNPVLVAK
                     QAATLDTLSGGRLTLGVGVGWSREEFDALGVPFERRAARTAEYVAAMRTLWRDDVASF
                     DGEFV"
     gene            complement(509..934)
                     /locus_tag="VFC01_26890"
     CDS             complement(509..934)
                     /locus_tag="VFC01_26890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83276.1"
                     /translation="MDTARAVAVATVFVWLGMVLAISLIETPLKFRAPGVTLQIGLGI
                     GRLIFRALNACELALAAVVAACFAVTSPTFGVAVAAAVAVVALLAQVLAVRPQLTRRS
                     DAVLAGGEGPRSSAHWVYVGLEIVKVVALLIAGVLLLAA"
     gene            complement(944..1303)
                     /locus_tag="VFC01_26895"
     CDS             complement(944..1303)
                     /locus_tag="VFC01_26895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HZN83277.1"
                     /translation="MSASRRIRVARVYDDPGPDDGERVLVDRLWPRGFRKGDPRVGTW
                     MPKVAPSTELRKWYSHKAARFDEFAARYAAELETEEGAAALAELRDVLRGAPVTLVTA
                     ARDLDGSHVAVLTRLLS"
     gene            1323..1517
                     /locus_tag="VFC01_26900"
     CDS             1323..1517
                     /locus_tag="VFC01_26900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83278.1"
                     /translation="MDIDRAAELQRWQDSGAVWEVISRKGGGVTVALLRCDGGEEVDR
                     FSSDDPRLLAFIGDRQSSAD"
     gene            1520..2044
                     /locus_tag="VFC01_26905"
     CDS             1520..2044
                     /locus_tag="VFC01_26905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZN83279.1"
                     /translation="MDLGKLTFVPAHEAPELVAEPVRPHLRDGLWVSPIDPDLADTAA
                     FCEHYDIGLDISANCVVVEARRADREWFAACLVLATTRADVNGIVRKHLGARKISFAP
                     MDSAVSVTGMEYGGITPVGLPADWPLLVDLNVMDQQHVIIGSGIRGSKLLAATEVLAS
                     LPNTEVLDIAMRRT"
     gene            complement(2084..2935)
                     /locus_tag="VFC01_26910"
     CDS             complement(2084..2935)
                     /locus_tag="VFC01_26910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZN83280.1"
                     /translation="MSVWFVTGASRGFGAEIVEKALAAGHSVVATARDPRGVTARFPD
                     AGEQLLPVALDVLDEAQAAAAVDAAVQRFGRIDVVVNNAGRGLLSAVEEASDAAARAV
                     FDTNVFGILNVLRAVLPTLRAQRSGRIVNLSSIGGFTASAGWGIYCATKFAVEGFSEA
                     LAHEVGPLGIDVIIVEPGYFRTDFLDASSLSTEGTVIDDYAETAGATRRHATDVNHAQ
                     PGDPVKAAAAIVEIASAERPPLRVQLGRDSFGGVADKLRFVADEQQAWRDLSVSTDHD
                     DVAVGRT"
     gene            complement(2955..3698)
                     /locus_tag="VFC01_26915"
     CDS             complement(2955..3698)
                     /locus_tag="VFC01_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN83281.1"
                     /translation="MIQAVDRALRILTVLQGGRRMSLGEIATAIDLAPSTVHGLVRTL
                     LAHGMVQQEIDSGRYRLGPATLRLGNVYLDTLELRSRVAIWAEGLARRTGCAVRTAVL
                     LFDEVVVVAHEPRPDGTRQMPEVGIVIPAHASALGKALLAFQSDYKADELRSMTGETI
                     IDPVALADQLEQIRSSGIATEAEEAVLGECAAAAPVFDSSGEATGAIGLVVPAARWPL
                     EAEAIDALRDTARTVSRELGSPVWPPRRV"
     gene            3844..>4356
                     /locus_tag="VFC01_26920"
     CDS             3844..>4356
                     /locus_tag="VFC01_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HZN83282.1"
                     /translation="MDEPSLTQKLAAEAFGTAFLVFIGVGSVPATIIVNGDAPFTMAD
                     LGMISFAFATVVVATIYALGHISGNHINPAVTLGLAVTGKFPWSRVPAYIAAQVVGAI
                     VGAAAILGVLGMAARDAGLGVATYSGGVSAVQAFFAEFVGTFILVFTVFGVIHRKASA
                     GFAGVAIGLVV"
BASE COUNT          752 a         1524 c         1410 g          670 t
ORIGIN      
        1 gcacgaactc gccgtcgaac gacgcgacgt cgtcgcgcca caacgtccgc atcgcagcga
       61 cgtactcggc ggtgcgcgcc gcccgccgct cgaacggaac gccgagtgcg tcgaactcct
      121 cgcgtgacca gccgacgccg acgccgagcg tgagccggcc gccggagagg gtgtccagtg
      181 tggccgcctg tttagcgaca agcacgggat tgtgctccgg caggagcagt acccctgtcg
      241 cgattccgat cgtggacgtg gccgcggcgg cgaagctcag cccgatcagc gggtcaatcc
      301 agtcggccgt ggccggcacg gcgatctgtc catcgtcgga gtacggatag cgcgacgccg
      361 accggtccac catgacgacg tgctcgcccg accacagcgt cgcgaaacca gacttttcgg
      421 cggcagccgc gacggcgtcg atgacgctgc gggtcgcgcc cgaaccgatg ccaagtgcgt
      481 gcagtcccaa ccgcatccgg cgatcgtctc acgcggcgag cagcaagacc cccgcgatca
      541 gcaacgcaac caccttgacg atctccagac cgacgtagac ccagtgcgcg cttgaccgcg
      601 gtccctcccc gccggcgagc acagcgtccg accggcgggt gagctgcggc cgcaccgcca
      661 gcacctgcgc cagcagggca acgacggcga cggctgccgc gaccgcgacg ccgaaggtcg
      721 gcgacgtcac tgcgaagcac gcggcaacga ctgcggccag cgcgagctcg caggcgttga
      781 gtgcccgaaa gatcaagcgg ccgatgccaa gtccgatctg cagcgtgaca ccgggcgccc
      841 gaaacttcag cggggtctct atcaacgaga tcgccaacac catgcccagc cagacgaaca
      901 ccgtggcaac agcgacggca cgagctgtat ccacggctgg agcctacgac agcaggcgag
      961 tcagaactgc gacgtggctg ccgtccagat cgcgggcggc agtcacgagc gtgacggggg
     1021 cgcctcgcag cacgtcgcgc aactcggcca gcgccgcggc cccctcctcg gtctccagtt
     1081 ctgcggcgta gcgcgcggcg aactcgtcga accgggcggc cttgtgggaa taccacttgc
     1141 gaagctcggt cgacggcgcg acctttggca tccacgtgcc gacccgcggg tcaccctttc
     1201 ggaaaccccg cggccacagc cggtcgacca gcacccgctc accgtcgtcg ggaccgggat
     1261 cgtcgtacac ccgcgcaacc cggatgcggc gactggcact catgcggtgg aatcgtagaa
     1321 ggatggacat cgatcgggcc gcagaactgc agcgctggca ggactccggc gcggtgtggg
     1381 aagtgatctc ccggaagggc ggcggtgtga cggtggccct gttgcgctgc gacggcggcg
     1441 aggaggtcga ccggttcagc tccgacgatc cccggctgct ggccttcatc ggcgatcgac
     1501 agagcagcgc ggactgacca tggacctggg aaagctgacc tttgtcccgg cgcatgaagc
     1561 gcccgagctg gtcgccgaac ccgtgcggcc acacctgcgc gacgggcttt gggtcagccc
     1621 gatcgacccc gacctcgccg acaccgcggc attctgcgag cactacgaca tcggcctgga
     1681 catctcggcc aactgtgtcg tcgtcgaggc gcggcgcgcc gaccgggaat ggttcgcggc
     1741 ctgtctcgtg ctcgccacga cacgcgccga tgtcaacggc atcgtccgca aacaccttgg
     1801 tgcgcgcaag atttcattcg ccccgatgga cagtgcggtg tccgtcaccg gaatggaata
     1861 cggcggcatc acccccgtcg gcctgcccgc agactggcca ctactggtgg acctgaacgt
     1921 gatggatcag cagcacgtga tcatcgggag cgggattcgc gggtcgaaac tattggccgc
     1981 gacggaggtt ctggcgtcgc tgccgaacac ggaggtgctg gacattgcga tgcgtcgaac
     2041 gtgagcttgt gctcgaagga tcgcgaaaat ctcgcacaca agctcacgtt cggccaaccg
     2101 ccacgtcgtc gtgatccgtt gacaccgaca ggtcgcgcca ggcctgctgc tcgtcggcga
     2161 cgaaccgcag tttgtcggcg accccgccga aggaatcgcg gcccagctgg acgcgcaacg
     2221 gcggccgctc cgccgaagcg atttcgacga tcgccgcggc ggccttgacc gggtcgccgg
     2281 gctgggcatg gttgacatcg gtggcgtgcc gcctggtggc gcccgcggtc tcggcgtagt
     2341 cgtcaatgac ggtgccttcg gtgctcaagc tcgacgcatc gaggaagtca gtacggaagt
     2401 agccgggctc cacgatgatg acgtcgatgc cgagcggacc cacctcgtgc gcaagcgctt
     2461 ccgagaaccc ctcgaccgcg aacttggtgg cgcagtagat accccagccg gccgaggcgg
     2521 taaatccgcc gatcgacgac aggttcacga tccgccccga ccgctgcgcc cgcagtgtcg
     2581 ggaggaccgc gcgcagcaca tttagaatgc cgaagacgtt ggtgtcgaac accgcccgcg
     2641 cggcggcgtc actggcttcc tcgaccgccg acagcaggcc tcggccggcg ttgttgacca
     2701 ccacatcgat gcggccgaac cgctgcacgg ccgcatccac cgccgcggcg gcctgcgcct
     2761 cgtccagcac atcgagcgcc accggcagga gctgctctcc cgcgtcaggg aaccgggccg
     2821 tgacgccgcg cggatcccgt gcggttgcga cgaccgaatg ccctgcggca agcgcctttt
     2881 cgacgatctc cgcgccgaag ccgcgcgacg caccggtgac gaaccacacg ctcatgacct
     2941 catcctttcg attgttagac ccggcgtggc ggccacaccg gcgatccgag ttcacgtgac
     3001 accgtgcggg cggtgtcgcg cagcgcgtcg atagcctcgg cttccaacgg ccagcgtgcc
     3061 gcgggtacca cgaggccgat tgccccggtc gcctcgccgg aggaatcgaa aaccggtgcg
     3121 gctgcggcgc attcgcccaa cactgcttcc tccgcctctg tcgcgatgcc ggacgagcgg
     3181 atctgctcca actgatcggc gagcgccacc ggatcgatga tggtttcacc ggtcatgctg
     3241 cgcaactcgt cggccttgta gtcggattga aaggccaaca gcgccttacc aagagcgctc
     3301 gcatgcgccg gaatcacgat gcccacctcg ggcatctgac gagtgccgtc gggacgtggc
     3361 tcgtgcgcaa ccaccacgac ctcgtcgaag agcaagacgg cggtgcgcac cgcgcagccg
     3421 gtccgtcggg ccagaccctc ggcccagatc gccacccgtg agcgcagttc cagcgtgtcg
     3481 agatagacgt tgccgagccg taacgtcgcc ggcccgagtc ggtagcgacc cgagtcgatt
     3541 tcctgctgga ccatgccgtg cgccaatagc gtgcgaacca gaccatggac ggtcgacgga
     3601 gccaggtcga tggcggtcgc gatctcgccc aggctcatcc gccggccgcc ctgcagcacc
     3661 gtgaggatgc gcagcgcgcg atccacggcc tggatcatcc gtccaccttc cgccgaaatc
     3721 ggcccttgtg atgaccgaag cataagcgta tggtccagat cacattcgac attatcgaat
     3781 gctatccgag atagtcgaca accacacggc agccggcttc gacccaggtc tcaggagaac
     3841 gtcatggacg aaccttccct cacccagaaa ctcgcggccg aagcattcgg caccgccttc
     3901 ctggtcttca tcggcgtggg atcggtgccc gccacgatca tcgtcaacgg ggacgcgccg
     3961 ttcacgatgg ccgacctcgg catgatctcg ttcgcgttcg ccaccgtcgt cgtggccacg
     4021 atctacgcgc tcggacacat ctccggcaac cacatcaacc cggcggtcac cctcggactc
     4081 gcggtcaccg gcaagttccc gtggtcgcgc gtgcccgcct acatcgccgc gcaggtcgtc
     4141 ggcgcgatcg tcggcgcggc cgccatcctc ggcgtgctcg gcatggccgc ccgcgacgcc
     4201 ggccttgggg tcgcgacgta ttccggtggt gtgagtgcag tacaggcctt tttcgccgag
     4261 ttcgtgggta cgttcattct tgtgttcacg gtcttcggcg tgatacatcg caaggcctcg
     4321 gcgggcttcg ccggcgtcgc catcggtctg gtggta
//
LOCUS       DASRJA010000606         7813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_943647,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000606 DASRJA010000000
VERSION     DASRJA010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7813
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943647"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VFC01_26925"
     CDS             <1..740
                     /locus_tag="VFC01_26925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN83283.1"
                     /translation="KLAAAGATTLLVARTADKLDELAATITDSGGRAVVYAADLTDEP
                     TVDDLVNRITEDHGALDIIVNNAGKSIRRSLHLQYERPHDFVRTIDVNYLGPIRLLLG
                     LIPAMRQHGSGHIVNISSIGVRVPPGPRWGAYQASKGAFDTWLRSVAPELHDDGVDVT
                     SVYMGLIRTRMVEPTPTLRNVPGLSAEQAADIVAKAIIERPATIEPWWTWPAEVTSVL
                     LRGPIDRAAKLWYRYTTDSGDARQAGR"
     gene            737..2293
                     /locus_tag="VFC01_26930"
     CDS             737..2293
                     /locus_tag="VFC01_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN83284.1"
                     /translation="MITGSVVTTAGRALLRSRLLTPMAPGALVGVLREAARGRTNPFT
                     LLAVAAARWPDRPAMIDDDGEITYGELRSRSEAVAKELRGVGPGQAVGILCRNGRAFI
                     PAVFGPALLGADVVLLNTDFHTDALAAVLTTHRIKTVICDDEFAERARAADASVTVVN
                     PSAVDTHQSRRPNVAAAGRIVLLTSGTTGKPKGVPRKPEIASALGIGASILDRTGLRV
                     GSRIAVPVPMFHGLGFGMLILTMTLGGTVLTLRRFDAEATLAKASQHRADALTAVPVM
                     LARILDLPEDVRDRHPLPSLRVVISSGARLDPTLARRFMDAYGDVIYNGYGSSEVGIG
                     SLATPADLREAPETVGRPVAGCPVRILDEHGKPVGPQVTGRVFVGGELTFDAYTGGET
                     KDVVADMTGTGDMGYFDEAGRLYIVGRGDDMIVSGGENVYPRAVENALAEHPDVADNA
                     VIGVPDDDYGERLAAFVVPRAEAELDTTALRNYLKDKVSRYEQPRDIEIVTKIPRNPA
                     GKVLRGELQT"
     gene            complement(2297..3442)
                     /gene="nagA"
                     /locus_tag="VFC01_26935"
     CDS             complement(2297..3442)
                     /gene="nagA"
                     /locus_tag="VFC01_26935"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HZN83285.1"
                     /translation="MLLTADTLLTGRELLRPGWVDVSDGVVRAVGAGPALRRADRELG
                     AVTVVPGFVDTHVHGGGGGNFSAASHADTSTAVALHRRHGTTTLVASLVTADPAELLC
                     QVTELADDVSAGLLAGIHLEGPWLSIKRCGAHQPSLMRDPDPAEIDRVLEAGGGAIRM
                     VTLAPERDGALAAIERFVERGVVAAVGHTEATYEQTRAAIAAGATVGTHLFNAMRPIH
                     TREPGPVIALVEDARVTVELIADGVHVDPALYRHVSHSAGPDRVSLITDAMAATGMAD
                     GVYHLGPVAVDVVDGVARVAGTDTIAGSTATMDRVFRFAVAHSGLPRDEALMLAVRQA
                     SINPARALGLPCMGLVPGAAADLVVLDADLAVAGVVHQGSWVDPAAC"
     gene            complement(3451..3921)
                     /locus_tag="VFC01_26940"
     CDS             complement(3451..3921)
                     /locus_tag="VFC01_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HZN83286.1"
                     /translation="MSTTPVLAPVAGRAVPLADVPDPVFSKGMVGYGAAIDPPRGVID
                     AVAPVSGKLLKLMPHAYIVMTPENVGVLVHLGLDTVQLKGDGFTTHVTQGDDVTAGQV
                     VITYDVPSVEAKGLNPIVPVVVMDEREPANVVPSEAVFEGAEIASGASLFTANK"
     gene            4037..5614
                     /locus_tag="VFC01_26945"
     CDS             4037..5614
                     /locus_tag="VFC01_26945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HZN83287.1"
                     /translation="MSDTAKPQGAAAKSSRRIPGFAQLQRLGKSLMLPIAVLPAAGLL
                     LRLGQPDVLGKIHAPVISSFFQAMSAAGDALFSNLPLLFAVGVAIGFARKADGSTALS
                     AVVGYLVVQAVFKTMSPIVLAGEIDKSGDQAQINYSVFAGIVVGLLTAWLFDRYHTIQ
                     LPSYLGFFGGRRFVPIVVSVASLFLAFAMSYFYPIFDAGLTGLGKFIAGSGALGAFVY
                     GFANRMLIPLGLHHIPNSYIWFLYGEYQTPGGEVVTGDIPRFTAGDPTGGAYTAGFFP
                     ILMFGLPAAALAMIHVANKKQRKVAIGILAAAALTAFVTGITEPLEFAFMFVAFPLYL
                     IHAVLTGLSLAIAYLLDIHLGFSFSAGLIDLLLYFTAPAAKNIPLLIGMGVVFFVIYY
                     LLFRFAITKWNLRTPGREPESEFEAEEEANLGEGGDAVTAVTAGGSGTATLTAPTATQ
                     SKAEQVIEAFGGRENLVNVDACITRLRVEVADKGKVDEAHLKRLGAAGVVEVGNSVQA
                     VFGTDAEALKNDITEIL"
     gene            complement(5622..6842)
                     /locus_tag="VFC01_26950"
     CDS             complement(5622..6842)
                     /locus_tag="VFC01_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN83288.1"
                     /translation="MSTEQLAPSGWSGHARGSTAYGRLLAALFCAGVATFAQLYSPQA
                     VLPLIAADLGVGAASAALMVSASTVGLAIGVIPWSTLADRIGRVKAISISVTGATTVG
                     LLVPFAPTFQLLLAGRFLEGLIVGGVPAIAVAYLTEEIDPAHAARAAGTFVAGTTIGG
                     LTGRLVASPVAEIAGWRVGVFTVAVLCGIAALSFVKLAPQPRGFVPSNRSGANPEGSL
                     AQRLFANLRSRHQLTLFAQAFLLMGGFVALYNYLGFRLSGAPFYLPPFVVSLVFLAYL
                     AGTWASSRAGAEASRFGRKPVLLVSVAVMIAGVAITLSDNVIAVLIGLVIATAGFFGA
                     HSIASGWTGRSAPVGKAQASSLYNLFYYGGSSVIGWFGGLAFDGYGWTAVAVTIIALA
                     VIAAAAAAVFLPAD"
     gene            complement(6937..>7813)
                     /locus_tag="VFC01_26955"
     CDS             complement(6937..>7813)
                     /locus_tag="VFC01_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN83289.1"
                     /translation="MHLEELEWFVALAEIEHVTEAAAELGVSQPTLSRALSRFEAQVG
                     TPLFDRVNRRLRLNPYGQIMLEHARRSITEMQSAIDRIDALRDPDTGRVRLAFLHSLA
                     SWYVPEQLRRFRESAQRVAFDLFQGPAHEIAQRVLSGQADIAITSPRPKMEGLVWHRL
                     YEERLCLAVPRGHRLASRARVRLSVAADEPFVALGAQAGLRQLTDELWAEDSIHPDIV
                     FEATEIPTIEGLVAAGFGVAVVPVPRDADEPKVVHVPLTNLGAKREVGLVWDRNRALS
                     PPGERFVTFLKATPA"
BASE COUNT         1391 a         2611 c         2544 g         1267 t
ORIGIN      
        1 acaagctcgc cgccgccggc gccacgacgt tgctcgtcgc tcgcacggcg gacaagctcg
       61 acgagctcgc agcgacgatc accgacagcg gcggccgagc ggtggtgtac gccgccgacc
      121 tgaccgacga gcccaccgtc gacgacctgg tgaaccgaat caccgaggat cacggcgcgc
      181 tggacatcat cgtcaacaac gcgggcaagt cgatccgccg gtcgctacat ctgcagtacg
      241 aacgaccgca cgatttcgtg cgcaccatcg acgtcaacta tctcgggcca attcgcttgc
      301 tactggggct gattccggcg atgcggcagc acggcagcgg gcacatcgtg aacatctcca
      361 gcatcggcgt gcgggttccg cccggtccgc ggtggggcgc ataccaggca tcgaagggtg
      421 cgttcgacac gtggctgcgc agcgtcgcgc cggagttgca cgacgacggc gtcgacgtga
      481 cctcggtgta catggggttg atccgcactc gcatggtcga gcccacaccg accctgcgca
      541 atgtgcccgg cctctccgcg gagcaggccg ccgacattgt tgcgaaggcc atcatcgaac
      601 ggccggccac gatcgagcca tggtggacgt ggcccgccga agtcacctcg gtgctgttgc
      661 gaggtccgat cgaccgcgcg gcgaagctgt ggtaccggta caccaccgat tccggtgacg
      721 cgcgacaggc cgggcgatga ttaccggaag cgtggtgacc acggccgggc gtgcgctgct
      781 gcgctcccga ttgctgacgc ccatggcgcc cggagcactg gtcggcgttc ttcgcgaggc
      841 ggcacgaggg cgcacgaatc cgttcacgct gctggccgtt gcagccgccc ggtggcccga
      901 tcggccggcg atgatcgatg acgacggtga aatcacctac ggcgaattgc ggtcaaggtc
      961 cgaagcggtc gccaaggagc tccgcggcgt tggaccggga caagcagtgg gaatcttgtg
     1021 ccgcaatggg cgcgccttca tcccagcggt gttcgggccg gccctgctcg gtgcggacgt
     1081 cgtgctcctc aacacggact ttcacactga cgcactggcg gccgtgttaa ctacgcatcg
     1141 aatcaagacg gtgatctgtg acgacgaatt cgccgaacgc gccagggccg cagacgcttc
     1201 cgtcaccgtc gtcaatccca gcgcggtcga cacacatcaa agtcgacgac ccaacgtcgc
     1261 ggcggccggt cggatcgtgc ttctcacctc cgggaccacg ggcaagccca aaggcgttcc
     1321 ccgcaagccc gagatcgcgt cggcactggg cataggcgcg tcgatcctcg atcgcacggg
     1381 actgcgggtc ggttcgcgaa tcgctgtgcc ggtgcccatg tttcacggcc tgggattcgg
     1441 gatgttgatc ctgacgatga ccctcggcgg gacggtgctg acgttgcggc gcttcgacgc
     1501 cgaagccacc ctcgccaagg cgtcgcagca ccgggccgac gcattgactg cggtgccggt
     1561 gatgctcgca cgcatcctcg acctacccga ggacgtgcgg gaccgtcacc ccttaccgtc
     1621 cttgcgtgtg gtgatttcaa gcggagccag gctggatccc accctggcgc gccgcttcat
     1681 ggacgcatac ggcgacgtga tctacaacgg ttacggctcg tcggaagtcg gcatcggttc
     1741 cctcgcgaca ccggcagacc tgcgcgaagc gcccgagacg gtgggcaggc ccgtcgccgg
     1801 gtgccccgtg cggattctcg acgaacacgg caaacccgtt gggccgcaag ttactggtcg
     1861 cgtcttcgtc ggcggcgagt tgacgttcga cgcctacacc ggcggggaaa caaaggacgt
     1921 ggtcgccgac atgaccggca ccggcgacat gggttacttc gacgaggcgg gtcggttgta
     1981 catcgtgggc cgtggggacg acatgattgt ctccgggggc gagaacgtct acccgcgcgc
     2041 cgtcgagaac gccctcgccg aacatcccga tgtcgccgac aatgcggtga tcggtgtgcc
     2101 ggacgacgac tacggtgaac gactcgccgc attcgtcgtc ccccgtgcgg aggccgagct
     2161 ggacacgacc gcgcttcgga attacttgaa ggacaaggtg tctcgctatg aacaaccgcg
     2221 tgacatcgag atcgtcacca agattcctcg caacccagca ggcaaggtat tgcgaggcga
     2281 gctgcagaca taggcgtcag caggcagcgg ggtcgaccca cgatccctgg tgcaccacac
     2341 ccgccactgc gaggtccgcg tccagcacca ccaagtccgc ggcagccccc gggacgagtc
     2401 ccatgcatgg cagaccgagc gcacgcgccg ggttgatcga cgcctgacgc acggcgagca
     2461 tcagcgcctc gtctcggggc aaaccgctgt gcgccacagc gaaccgaaac acccggtcca
     2521 tggtcgccgt gctgcccgcg atggtgtccg tgcccgccac tcgcgccacc ccgtctacga
     2581 cgtccaccgc taccggtccg aggtgataga ccccgtcggc catcccggtc gccgccatcg
     2641 cgtcggtgat cagcgacacc cggtcagggc ccgcgctgtg cgagacatgt cggtacaggg
     2701 cggggtcgac gtgcacaccg tcggcgatca gttccacggt cacccgggcg tcttcgacaa
     2761 gcgcgatcac cgggccgggc tcgcgagtgt ggatcgggcg catcgcgttg aacaggtgcg
     2821 tgcccacggt cgcgcccgcc gcgatcgctg cccgcgtctg ttcataggtc gcctcggtgt
     2881 ggccgacggc ggccaccaca cccctttcga cgaagcgctc gatcgccgca agggcgccgt
     2941 cgcgttccgg ggccagggtc accatccgaa tcgccccgcc gcctgcttcg agtacgcgat
     3001 cgatctctgc aggatccggg tcacgcatga gcgacggctg gtgggcgccg caccgcttga
     3061 tggacagcca gggcccttcc aggtggattc cggcgagcag cccggcactc acgtcatcgg
     3121 ccaattccgt tacctggcac aacaattcgg ccgggtcggc ggtgactagc gaggcgacca
     3181 gggtggtggt gccgtgccta cggtgcaatg ccacggcggt ggacgtgtcg gcgtgcgatg
     3241 cggcggaaaa gttcccgcca cctccgccgt gcacatgggt gtcgacgaag cccggtacca
     3301 ccgtgaccgc accgagttcg cgatccgcgc gccgcagcgc tgggccggca ccaaccgcac
     3361 gcacaacccc gtcggacacg tcaacccacc cgggccgcaa cagttctcgc ccggtgagca
     3421 gggtgtcggc ggtcagcaac acgctgtcgt ctacttgttc gcggtgaaaa ggcttgcccc
     3481 ggaggcgatc tcggcaccct caaacaccgc ttcggacgga acgacgttgg cgggttcgcg
     3541 ttcgtccatg accacaacgg gcacaatggg gttgagcccc ttggcctcta ccgacgggac
     3601 gtcgtaggtg atgacgacct ggcccgcggt gacgtcgtcg ccctgggtga cgtgcgtcgt
     3661 gaagccgtca cccttcaact gaacggtgtc aagacccagg tggaccagga ctccgacgtt
     3721 ctccggggtc atgacgatgt aggcgtgcgg catgagtttc aagagcttgc cgctgaccgg
     3781 agccaccgcg tcgatcaccc cgcggggcgg gtcgatggcg gcgccatagc ccaccatgcc
     3841 tttcgagaaa acgggatcag ggacgtccgc aagcggtacc gcacgccccg cgaccggggc
     3901 caggactggc gtcgtgctca cgcaccaaca atacgagcgt gccgcgaaga cggtctcagg
     3961 ttcaccggtt ctggtctagg cttccgctga gcttgggcgg cgggacggcg gttgcggcag
     4021 aggagggaca gaggcaatga gcgatacggc gaaacctcaa ggggcggccg caaagtcgag
     4081 tcggcgcatt cccggcttcg cgcaactgca gcggctgggc aagagcctga tgctgccgat
     4141 tgcggtgttg ccggccgcgg gccttctgct gcggctgggc cagcccgacg tgctcggcaa
     4201 gattcacgcg ccggtgatca gctccttctt ccaggcgatg agcgccgcgg gcgacgcgct
     4261 gttcagcaac ctcccgctgc tcttcgccgt cggcgtcgcc atcggattcg ccaggaaggc
     4321 ggacggttcg accgcgctgt ccgccgtcgt cggctacctc gtggtgcagg cggtgttcaa
     4381 gacgatgtcg ccgatcgtgc tcgcaggcga gatcgacaaa tcgggcgatc aggcccagat
     4441 caactacagt gtgttcgcgg gcatcgtggt cggcctgctc accgcttggc tcttcgaccg
     4501 ctaccacacc attcaattgc cctcctacct cggattcttc ggcggtcggc ggttcgttcc
     4561 gatcgtcgtg tcggtggcgt cgctcttcct cgcgttcgcg atgagctact tctatccgat
     4621 cttcgacgcc ggactgaccg gcctcggcaa gttcatcgcg ggatcggggg ctctcggcgc
     4681 ttttgtttac gggttcgcca accgcatgct gattccgctc ggcctgcacc acattccgaa
     4741 ctcctacatc tggttcctct acggcgagta ccaaactcct ggcggcgaag tcgtcaccgg
     4801 cgacattccc cgcttcaccg cgggcgaccc cacgggcggc gcctacaccg ctggcttctt
     4861 ccccatcctg atgttcgggc tgcccgccgc cgcgctggcg atgatccacg tggccaacaa
     4921 gaagcagcgg aaggtcgcca tcggcatcct ggcggcagct gcgctaaccg cgttcgtgac
     4981 aggcataacc gagcctctcg agttcgcgtt catgttcgtg gcgttcccgc tctacctcat
     5041 tcacgccgtt ctgacggggc tgtccctggc gatcgcgtac ctgctcgaca tccacctcgg
     5101 attctctttc tccgcgggcc tcatagatct actgctctac ttcacggcgc ccgcggcaaa
     5161 gaacatcccg ctgctcatcg gtatgggtgt tgtcttcttc gtcatctact acctgctgtt
     5221 ccggtttgcg atcacgaagt ggaacctgag aactccaggg cgcgagccgg agtccgaatt
     5281 cgaagctgag gaagaagcga acctcggcga gggcggcgac gcggtcaccg ctgtgacggc
     5341 aggcggatcc ggcacagcga cattgaccgc acccaccgcg acgcaaagca aggccgagca
     5401 ggtcatcgag gctttcggcg gccgggagaa tctcgtgaac gtcgacgcct gcatcacgcg
     5461 cctgcgggtg gaggttgccg ataagggcaa ggtggacgag gcgcatctga agcgcctcgg
     5521 ggcggccggt gtcgtcgagg tgggcaacag cgtccaagcg gtgttcggta ctgacgccga
     5581 ggccctgaag aacgacatca ccgagatcct ctagccgcgg gctaatcagc cggcaggaac
     5641 actgctgccg cggcggctgc gatgacggcc agcgcgatga tcgtcaccgc gacagcggtc
     5701 cagccgtacc cgtcgaaggc cagcccgccg aaccagccga tgacgctcga accgccgtag
     5761 tagaacaggt tgtacagcga ggatgcctgc gctttgccca ctggcgccga tcgacccgtc
     5821 catcctgacg caatcgagtg tgcgccgaag aaccccgcgg tggcgatcac caggccgatg
     5881 agaaccgcga tgacgttgtc actcaacgta atagcgacgc cggcgatcat caccgcaacg
     5941 gacaccagca ggaccggctt gcgcccgaac cgcgacgcct ccgcaccggc gcgagacgat
     6001 gcccacgttc cggcgagata cgccaggaac acgaggctga cgacgaacgg tggtaggtag
     6061 aacggtgctc ccgacaaccg gaatcccagg tagttgtaca gcgccacgaa tccacccatc
     6121 aacaggaacg cctgggcgaa cagggtgagc tggtggcgcg agcgcaggtt ggcgaacaac
     6181 cgctgcgcaa ggctgccttc gggattggcg ccagaccggt tcgagggaac gaagcctcga
     6241 ggctgcgggg cgagcttgac gaagctcagt gcggcgattc cgcatagcac cgcgacggtg
     6301 aagacgccga cacgccagcc ggcgatttcg gcgactgggc tggccaccaa ccgtcccgtc
     6361 agcccaccga tcgtcgttcc agcgacgaag gtgcctgccg ccctggcggc gtgcgcggga
     6421 tcgatctcct cggtgaggta tgcgacggcg atggcaggca cgccgccgac aatcagccct
     6481 tcgaggaagc gcccggccag aagaagttga aaggtgggcg cgaacggcac cagaagaccg
     6541 acggtcgtcg cgccggtgac cgagatcgag atcgccttga cgcgaccgat acggtcagcc
     6601 agcgtcgacc acggaatcac tccgatcgcc aggccgacgg tcgaagccga gaccatcaat
     6661 gccgcactgg ccgcacccac acccaggtcg gctgcgatca gcggcagtac agcctgcggc
     6721 gaatagagct gagcgaacgt cgccactccg gcgcagaaca gcgcggcaag cagccggccg
     6781 taagcggtgg agccacgcgc atgcccggac cacccggatg gagctagctg ttcggtcgac
     6841 accattggca cggtaagcgc cgtgcagtga tgtgtctaat gcatgacttt ctgtatgttc
     6901 atactgctac cgcatcattg gtcggtagca ccgcaatcag gcgggcgttg ccttgagaaa
     6961 agtcacgaag cgctcccccg gcggagacag cgctcggttc cggtcccaca ccaaaccgac
     7021 ctcacgcttg gcgccgaggt tcgtgagcgg gacgtgaacg actttgggtt cgtcggcatc
     7081 gcgcggcacc ggcacgacgg cgacaccgaa tcccgcggcg acaaggcctt ctatcgtggg
     7141 aatctcggtg gcttcgaaaa cgatgtcggg gtggatgctg tcctcggccc acagttcatc
     7201 ggtgagctgc cgcagaccgg cctgggctcc aagcgcaacg aacggctcgt cggcggccac
     7261 ggatagccgc acacgggcgc gcgacgccaa ccggtgcccg cgcggaacag ccaggcacag
     7321 ccgctcctcg tacagccggt gccagacgag gccttccatc ttcggccgcg gcgaggtgat
     7381 ggcgatatcg gcctgaccgc tcaggacgcg ttgagcgatc tcgtgggcgg gcccctgaaa
     7441 caagtcgaac gcaacccgtt gcgccgactc gcggaagcgg cgaagttgtt ccggcacata
     7501 ccaactcgcc agcgaatgca gaaacgccag tcgcactcgt ccggtgtctg gatcccgaag
     7561 ggcgtcgatg cggtcaatag cggattgcat ctcggtgatg ctgcgccggg catgctcgag
     7621 catgatctgc ccgtacgggt tcaggcgcag ccgccgattg acccggtcga acaacggcgt
     7681 gcccacctgc gcttcgaatc gactcaaggc gcgcgacaac gtgggctggc tgacgcccaa
     7741 ctccgcggcg gcctcggtga cgtgctcgat ctcggcgagc gcgacgaacc actccagttc
     7801 ctcgaggtgc att
//
LOCUS       DASRJA010000607         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1199778,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000607 DASRJA010000000
VERSION     DASRJA010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199778"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFC01_26960"
     CDS             <1..692
                     /locus_tag="VFC01_26960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83290.1"
                     /translation="ETIDIVRRGANFGYPLREGPEFKPESPIYGPLSADNTLPLRISD
                     TVTLDQRVPMEDSALAYRTGVEGLAIANGFVYRGKRWPQLQGSVVFGDITSGRLFYAR
                     MADLIAATDGNPKTLAAYSEITTTLPTLVQERIRLNPPAPGFGAGGPGGPGPMPGAGP
                     GAGRTGEPGAVSPAGPGVVPPGGPGAAPAGGAFAPRPLRVEVRIATDEAGEIYILTKS
                     DGLIRRITSIR"
     gene            722..1873
                     /locus_tag="VFC01_26965"
     CDS             722..1873
                     /locus_tag="VFC01_26965"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN83291.1"
                     /translation="MSISTRPFIVVLGAALLATGLTTSARQEASPLPPLVLPAGFQAE
                     VFAEKVENARSMAIGPQGTVFVGSQYVGKVHAVVDGNRDQKADRVVVIASGLDQPNGV
                     AMRNGALYVATASRLLRFDDIEKHLDAPPAPVTLRDDLPNPKAGHTWKFIGFGPDDML
                     YMTVGAPCNVCLSPPMVSAIVRMKPDGSGMETFAEGVRNSVGFDWHPVTRELWFTDNG
                     RDMLGDDVPNDELNVAPKAGLHFGFPYCHQGDVTDPEFGAQRACSTTEAPVQKLGAHV
                     AAIGFAFYSGKMFPASYQNAAIIAQHGSWNRSTPSGYRVMVARTEGRRVISYEPLVEG
                     FLPKADGSGGRGATKVAAGRPVDVLQMPDGSILISDDRGNRLIRVSYRL"
     gene            2016..>2590
                     /locus_tag="VFC01_26970"
     CDS             2016..>2590
                     /locus_tag="VFC01_26970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83292.1"
                     /translation="MRTTRQTVSRLLLATAAVASLSVLVRAQDRKEVTINDTGTQAEN
                     LTSSQDGSVYFGSTAKGTIYRAAPGASQAEPWIQAATAGLTNVLGVLADDKSNTLWVC
                     QNNTGGRGGAPVVGQTALRSFDLKSGAAKATYPFPSNGGVCNDMAVAPDGTVYATESF
                     ANRIHRLKPGAKELDLWVPADPQLAGVDGIA"
BASE COUNT          458 a          849 c          843 g          440 t
ORIGIN      
        1 acgaaacgat cgacatcgtc cgtcgaggag ccaacttcgg atacccgctg cgagagggac
       61 cggaattcaa gccagagagc ccgatctatg gtcccctgag cgccgacaac accttgccac
      121 tgcgaatctc cgatacggtc actcttgatc aacgagttcc gatggaggac tcggcactcg
      181 cataccggac gggtgtcgag ggcctggcga tcgccaacgg cttcgtctat cgcggcaaga
      241 ggtggcctca gctgcaaggg tccgtcgtct ttggtgacat caccagtgga cgactgtttt
      301 acgcgcggat ggcggatctc attgcggcca cggacggcaa ccccaagaca ctagcggcgt
      361 actcagagat cactacgacc cttcccacac tcgtgcagga gcgcatccgg ttgaatccgc
      421 ctgcgcctgg attcggagcc gggggtccgg gtggacccgg gccgatgcct ggggccggcc
      481 ctggggctgg gcgtacaggt gaacctggag cggtgtcacc ggccggaccc ggagtggttc
      541 caccgggtgg acctggcgct gcgcccgcgg gtggggcctt cgctccccgg ccattgcggg
      601 tcgaagtgcg catcgcaaca gatgaggccg gagagattta catcttgacg aagagcgacg
      661 gcctcatccg ccgaatcacg agcatccggt gacactcagc acgacgcctg gaaaacagaa
      721 catgtccatc tcaacgcgtc ccttcatcgt cgttctcggc gccgccctgc tggccaccgg
      781 cttgaccacg tccgcccgcc aagaggcgtc gccgcttccg ccattggtgc tgcccgcggg
      841 atttcaggcg gaagtgttcg ccgagaaggt ggagaacgcc cgctcgatgg cgatcggccc
      901 gcaaggaacg gtgttcgtcg gctcccaata tgtcggcaag gtgcacgccg ttgtggatgg
      961 caacagggac cagaaggccg atcgcgtcgt ggtcattgcc agcggtctcg atcaacccaa
     1021 tggcgtcgcg atgcggaacg gcgccttgta tgtggcgacc gcgagccggc tcctgcggtt
     1081 cgacgacatc gagaagcacc tcgacgcgcc gcccgcgccg gtgactcttc gcgacgatct
     1141 gccgaacccg aaggccggcc acacgtggaa gttcatcggc ttcggcccgg acgacatgct
     1201 ctacatgacg gtcggcgctc cgtgcaacgt gtgtctctcg ccgccgatgg tgtcggccat
     1261 cgtgcggatg aagccggatg gatcaggcat ggaaaccttc gcagagggcg tgcgtaacag
     1321 cgttggattc gattggcatc cggtgacgcg cgagctgtgg ttcaccgaca acggccgcga
     1381 catgctgggc gacgacgtgc cgaacgacga gctgaacgtt gcgcccaagg caggcctgca
     1441 cttcggtttc ccctactgtc atcagggcga cgtcacggac ccggagttcg gggctcagcg
     1501 cgcgtgttcg acgaccgagg ctccggtgca gaagctcggg gcgcacgtcg cggcgatcgg
     1561 cttcgccttc tactccggca agatgttccc ggccagctac cagaacgcgg cgatcatcgc
     1621 ccagcacggt tcgtggaatc gatcgacgcc gagcggctat cgcgtcatgg tggcgcggac
     1681 cgagggccgc cgcgtgatca gctacgaacc gctcgtcgaa gggtttctgc cgaaagccga
     1741 cggctcgggc ggacggggcg ccaccaaggt cgccgccggc cgcccggttg acgtgctgca
     1801 gatgcccgac ggctcgatcc tgatcagcga cgaccgcggc aatcgcctga tccgtgtgag
     1861 ctaccgtctt taaagtcggg gccctgcccc gcaccccggc tcgtcgcttg cggggacccc
     1921 tgcgccccgc gccgctcctc gcaggcgcgc tgtgcgcgcc ttggctgcca gagttttctg
     1981 cagccacact tggccattac cgttcggaga ccgctatgcg caccactcgg cagactgtca
     2041 gtcgcttact tctcgccacc gccgctgtcg catcgctctc ggtgctcgtg cgagcgcagg
     2101 accgcaagga agtgacgatc aacgacaccg ggactcaggc cgagaacctg acgtcgagtc
     2161 aggatggctc ggtgtacttc ggcagcaccg cgaaaggcac gatctaccgc gccgcgccgg
     2221 gcgcgtcgca agccgaaccc tggattcagg cagcgaccgc cggcctgacc aacgtgcttg
     2281 gcgtgctcgc ggacgacaag agcaacacgc tgtgggtctg ccagaacaac accgggggcc
     2341 gaggcggtgc gcccgtggtc gggcagaccg cactgcgatc gttcgacttg aagagcggtg
     2401 ctgccaaggc cacttatccc tttccgagca atggcggtgt gtgtaacgac atggccgtgg
     2461 cccccgacgg cacggtctac gcgaccgagt cgttcgccaa tcggatccat cgcctcaagc
     2521 ctggtgcaaa agagctagat ctctgggtac ccgctgatcc gcaactggcc ggtgtcgatg
     2581 gcattgcgat
//
LOCUS       DASRJA010000608         4608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1199824,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000608 DASRJA010000000
VERSION     DASRJA010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199824"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VFC01_26975"
     CDS             <1..934
                     /locus_tag="VFC01_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005057486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HZN83293.1"
                     /translation="TVTGVDGKVRRTVNIRLRAHTMMHDFSLTEKYVVLYDLPVALDL
                     GGTLRSRPAKAVAGMLTRVAERHAAPDFVVRAIMRGSERSGPPSGGMPYRWAPERQAR
                     VGVLPREGTASEIRWFEVPQCYVFHPLNAYDDGDRVVLDVVRHASVFNSGLRIVPEKP
                     SLDRWTVDLTAGKVAEERLDDTPQEFPRVDERLVGRRHRYGYAVGYSDGTTGTPDAIL
                     KHDAATRRTQSVGFGAGREPGEFVFVPSTPDAAEDDGVAMGFVYDRSSDRSDLVLLDG
                     QTLETVATVHLPARVPHGFHGNWVPTEGLGPQRS"
     gene            948..2114
                     /locus_tag="VFC01_26980"
     CDS             948..2114
                     /locus_tag="VFC01_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZN83294.1"
                     /translation="MIGGRSGEWHWLWDAYVVGMCAAAAAGVVMLDHRFPGNVPVALI
                     ALAGVVVCVLAFGRRVIRGGEFDWRTLLFLAAVIGLWIVALSASSVAVAAVPAIYPLV
                     FASLPLPAALVVTTFVNFVPLSLALMFYGTRWPDLPLAVAFTLVGVIAAPVIGITIMT
                     SMKQREKLAALVAELATTRAESARLSREAGAATERERLAREIHDTLAQGFTSIVALAQ
                     AVEPELETDTAAAKRHVELIRSTARENLAEARTMVAELTPTALHEGSLRSAIQRQCDR
                     LSAESGIEATVRVDGELPPLGMATDVVLLRATQEAFANIRKHSEASTVTVALSPVEHG
                     IRLSVADNGIGLADEHNEGFGLRGMKARAKQVGGTMSVTPTPGGGTTVTVEVPA"
     gene            2111..2731
                     /locus_tag="VFC01_26985"
     CDS             2111..2731
                     /locus_tag="VFC01_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005103171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN83295.1"
                     /translation="MTTVLLVDDHPVVREGLRGMIDAEHDLTVVGEAGSGAEAIALAE
                     SLRPDVILMDLRMADVDGVTATERILAVLPRTRIVVVTTYESDTDILRAVEAGAAGYL
                     LKDASRAELADAVRDAARGKTVLAPTVADRLVHFVRQPASAALSAREVEVLGHVAKGE
                     SNAEIGRQLQISEATVKTHLLRTFNKLGVSDRTAAVTTAMSLGLLS"
     gene            complement(2720..3127)
                     /locus_tag="VFC01_26990"
     CDS             complement(2720..3127)
                     /locus_tag="VFC01_26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83296.1"
                     /translation="MSGRAAVVGRRACAVLAACSAVLHGFSLVHATSVAAIALTVVML
                     TACLFCARELWIRGTLRAWVVVALMNLAMIAIHAPASAAHHHGGGVSAHAPAHHSTVM
                     TLATTLAVVEVVVATAVLYYRTRGIRPALLSAE"
     gene            complement(3124..3867)
                     /locus_tag="VFC01_26995"
     CDS             complement(3124..3867)
                     /locus_tag="VFC01_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HZN83297.1"
                     /translation="MNTPPIVSAQEWEAARQQLLRQEKDVTRARDALAASRRRMPWLP
                     VDKHYEFDGPEGRVSLLDLFAGRRQLIVYRAFFEPGVHGWPDHACRGCSMVADNIGHV
                     AHLNARDTTLVLASRATQVDIERVKARMGWQMPWYTITGGFDVDFGVDQWHGTNAFIR
                     DGDSVFRTYFINNRGDEALGSTWSYLDMTALGRQELWEDSPEDYPQTPPYEWWNWHDE
                     YSDPESSSDEWKRRSAAAQALLRTNGETT"
     gene            complement(3978..>4608)
                     /locus_tag="VFC01_27000"
     CDS             complement(3978..>4608)
                     /locus_tag="VFC01_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN83298.1"
                     /translation="VYAAARQPVAHPDARVTPVTIDVTDAAQIQEALGKVESLDILIN
                     NAGLALYDDLSDPTPLEQQLAVNLFGTYRVTQAFLPALTRSRGAIVNVGSVTAFAALP
                     VIPAYSISKAAAFSLSQSMRALFAGRGVTVHAVLAGPVDTDMSRDLDIPKASPESVAR
                     AILDGVQNGEDDIFPDPMAASMAESWRTGAAKALERQFAALLDAQPVTP"
BASE COUNT          779 a         1476 c         1574 g          779 t
ORIGIN      
        1 caccgtcacc ggcgtcgacg gcaaagttcg ccgcacagtg aacatccgac tcagggcgca
       61 cacgatgatg cacgacttct cgctgaccga gaagtacgtc gtgctctacg acctgcccgt
      121 cgcgctggac cttggcggaa cgctgcggag caggcccgcc aaagccgtcg ccggaatgct
      181 gacgcgggtc gccgaacgcc acgccgcacc ggatttcgta gtgcgcgcga tcatgcgtgg
      241 atcggagcgc tcgggtccgc cgagcggcgg catgccatac cggtgggcgc cggaacgaca
      301 ggctcgcgtc ggggtgctgc ctcgcgaggg cacagcatcc gagatccgct ggttcgaggt
      361 gccgcagtgc tacgtcttcc atccgctcaa tgcctacgac gacggcgacc gcgtcgtgct
      421 cgatgtcgtc cgccacgcgt cggtattcaa cagcggcctg cgcatcgtcc ccgaaaagcc
      481 atcgctcgac cggtggaccg tcgatctcac cgccgggaag gtcgccgaag aacggctcga
      541 tgacacgccg caggagttcc cgcgtgtcga tgagcgattg gtcggccggc gccaccggta
      601 tggctacgcg gtcgggtact ccgacggaac cacgggaaca cccgacgcga tcctcaaaca
      661 cgatgccgca acacggcgca cccaatcggt ggggttcgga gccggccgtg agcctggcga
      721 gttcgtcttc gttccctcga cgcccgacgc cgccgaagac gatggtgtgg ccatgggttt
      781 tgtgtacgac cgctcgtccg accgaagcga cctggtgctg ctggacgggc agacactgga
      841 aaccgtcgcg accgtgcacc ttccggcccg ggtgccgcat ggttttcacg gcaactgggt
      901 gccgaccgag ggtttgggcc cacagcggag ctaggggaca tcgcccgatg atcggcgggc
      961 gttccggtga gtggcactgg ctgtgggacg cctacgtcgt gggcatgtgc gccgcggccg
     1021 ccgcgggggt cgtcatgctc gaccaccgct tcccaggcaa cgtgccggtg gcgctgatag
     1081 cgttggcggg cgtcgtcgtg tgtgtattgg cgttcggccg ccgcgtcatc cgcgggggcg
     1141 aattcgattg gcgaaccctg ctgtttctcg ccgccgtgat cggactgtgg atcgtcgcgt
     1201 tgtcagcgtc gtcggttgcg gtcgccgcgg tgcctgcgat ctatcctctc gtattcgcct
     1261 cattgccgct ccccgcggcg ctcgtcgtga caacattcgt gaacttcgta ccgctaagcc
     1321 tcgccctgat gttctacgga acccgttggc ccgatctgcc tctggcggtt gcctttacgc
     1381 tcgtcggggt aatcgctgca cccgtcatcg ggattacgat catgacctcg atgaagcaac
     1441 gcgagaagct ggccgcactc gtcgcagagc ttgcaacgac ccgcgccgag agcgctcggc
     1501 tgtcgcgcga agccggcgca gccactgaac gggaaaggct ggcacgcgaa atccacgaca
     1561 cgttggctca gggattcacc agtatcgtcg cgctcgcgca agccgtcgag cccgagttgg
     1621 agaccgacac ggcagctgcg aagcggcacg tcgaactcat ccgcagcacc gcacgggaaa
     1681 acctcgcaga ggcgcggacg atggtggccg agctgactcc gactgcgctc catgaaggtt
     1741 cactgcgatc cgccatccaa cgtcagtgcg acaggctctc ggcagagagc ggcatcgagg
     1801 ccacggtccg cgtcgacggc gagctgccac cactgggcat ggcgaccgat gtggtgctgc
     1861 tgcgcgccac gcaggaagca ttcgccaaca tccgcaagca ttccgaagcg agcacggtga
     1921 cggtggcgct ttcgcccgtc gagcacggca tccgattgtc ggtcgccgac aacggtattg
     1981 gcctcgccga cgagcacaat gagggattcg gtctgcgtgg tatgaaggcc cgcgccaagc
     2041 aggtgggcgg cacgatgtcg gtgacgccga cgcctggcgg cgggacgacc gtgacggtcg
     2101 aggtcccggc atgacaacgg tgctattggt cgacgaccac cctgtggtcc gcgagggtct
     2161 gcgcgggatg atcgacgcgg aacacgacct caccgtcgtc ggtgaagcgg gttcaggcgc
     2221 cgaggcgatc gcgctggccg aatctctgcg cccagacgtc attctgatgg atctgcggat
     2281 ggccgacgtc gacggcgtga cggcgaccga gcggatcctg gcggtgctgc cgcgcacgcg
     2341 gatcgtcgtc gtcaccactt atgagtccga caccgacatc ctgcgcgcag tagaagcggg
     2401 cgcagcgggg tatctgctca aagacgcctc gcgtgccgag ttggccgatg ccgtgcgtga
     2461 tgccgcacgc ggtaagacgg tgctggcgcc gacggtcgcg gaccggctgg ttcactttgt
     2521 gcgccaaccg gcttcggcgg cgctctccgc acgtgaagta gaagtgctcg gccacgtcgc
     2581 gaagggcgag tcgaacgcgg aaatcggtcg tcagctacaa atcagcgagg ccactgtgaa
     2641 aacccatctg ctgcggacgt tcaacaaact cggcgtctcc gaccgcacag cagcggtgac
     2701 gactgcaatg tcgctgggct tactcagctg acagaagcgc aggccggatt ccgcgggttc
     2761 ggtagtacaa caccgcagtg gcgacgacga cctccaccac cgcgagcgtc gtggcgaggg
     2821 tcatcaccgt cgaatggtgt gccggcgcat gcgcgctgac accgccgccg tggtgatggg
     2881 cggccgatgc gggggcatgg atggcgatca tcgcgaggtt catcaaggcc accaccaccc
     2941 aggcccgcag cgtgccgcga atccacagct cacgggcgca gaacagacaa gcggtgagca
     3001 tcaccacggt cagcgcgatc gccgcgacgc tcgttgcgtg cacaagcgaa aagccatgca
     3061 gcacagccga acaggcggcg agcaccgcac atgctcgccg cccgacgacg gccgcgcgac
     3121 cgctcacgtg gtctcgccgt tcgtgcgcag caaggcctgc gcggcagctg accgccgctt
     3181 ccactcgtcg gacgacgact cggggtcact gtattcgtcg tgccagttcc accactcgta
     3241 gggcggcgtc tgcgggtagt cctccggtga gtcttcccac agttcctggc gtccaagcgc
     3301 ggtcatgtcg aggtagctcc acgtactgcc caaggcctcg tcgccgcggt tgttgatgaa
     3361 gtaagtgcgg aacacgctat cgccgtctcg gatgaacgcg ttggtgccgt gccactggtc
     3421 cacgccgaag tccacgtcga agcctccggt gatggtgtac cacggcatct gccaacccat
     3481 ccgggccttc acccgctcga tgtcgacttg cgttgcgcgc gaggccaata cgagtgtggt
     3541 gtcgcgggcg ttcaggtgag cgacgtggcc gatgttgtcg gcaaccatgg agcagccgcg
     3601 gcaggcatgg tcgggccagc cgtgcacgcc gggttcgaaa aaggcgcgat agacgatgag
     3661 ttgacgtcgg ccggcgaaca ggtccagcag gcttaccctg ccctcgggcc cgtcgaactc
     3721 gtagtgcttg tcgacgggca gccacggcat ccgtcggcgc gaggcggcca gcgcgtcacg
     3781 ggcgcgggtg acgtcctttt cctggcggag cagctgctgg cgcgcagcct cccactcctg
     3841 cgctgacacg atcgggggtg tgttcatttt cgatttcggc ctttccgttg gtggggatat
     3901 cgctgtctgt atggacgacg gcggatccat gaactgggcg cagagggccc gccgactttt
     3961 ctcccgatgg tcgggagtca cggcgtgacg ggctgcgcgt ccaggagtgc cgcgaactgc
     4021 cgctcgaggg ccttggccgc accggtgcgc cagctctcgg ccatcgacgc cgccatggga
     4081 tcggggaaga tgtcgtcctc cccgttctga actccgtcga ggatggctcg ggctacggac
     4141 tccggcgatg ctttcggtat gtcgaggtcg cgggacatat cggtgtccac tgggccagcg
     4201 aggacggcat gcaccgtcac cccgcgtccg gcgaagagag cgcgcatcga ttgcgacagg
     4261 gagaacgcgg ccgccttcga gatcgagtag gccggaatga ctgggagggc ggcaaaggcg
     4321 gtcaccgacc cgacgttgac aatggccccc cgagaacgcg taagtgccgg gaggaaagcc
     4381 tgggtcacgc gataagtgcc gaacaggttg acggccagct gctgttcgag cggtgtggga
     4441 tcgctcaagt cgtcgtagag cgccaagccg gcgttgttga tgaggatgtc gagagactcg
     4501 accttcccga gggcttcctg gatctgagcg gcgtcggtca cgtcgatggt cacgggcgtg
     4561 acgcgggcgt ccgggtgggc cacgggttga cgcgctgcgg cgtaaacc
//
LOCUS       DASRJA010000609        15647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3394295,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000609 DASRJA010000000
VERSION     DASRJA010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15647
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3394295"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFC01_27005"
     CDS             <1..238
                     /locus_tag="VFC01_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04338 family metallohydrolase"
                     /protein_id="HZN83299.1"
                     /translation="AAHYERSDDAAAIAVPDRRSTWALRELVVLHEIAHHLSDAQPPH
                     GPEFVATFCELAEAVMGPEVAHVLRVVYAKEGVR"
     gene            249..923
                     /locus_tag="VFC01_27010"
     CDS             249..923
                     /locus_tag="VFC01_27010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HZN83300.1"
                     /translation="MADPDPKALDELFTRVLDTEDDALRAAREAGVAADMPAIEVSAQ
                     HGKLLFLLVTAMRATRVLEIGTLAGYSTIQLARAVGPGGRVITLEYEPKHAEVARENL
                     ARAGVDDRVEVIVGVALDTLPTLAGRDETFDLIFIDADKENNVAYVEWAIKLGAPGSI
                     IVVDNIARFGRVLDPAPDDLQARAVRDMLEMMGANPRLEAAAIQTVGTKGWDGFAVAI
                     VKEPGE"
     gene            1251..1772
                     /locus_tag="VFC01_27015"
     CDS             1251..1772
                     /locus_tag="VFC01_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83301.1"
                     /translation="MKFALGAVLAASSALAVSPVAHADRAEPPPLYGYYNVSIDFSKQ
                     TFNGMPTPMNSITVPVEFTTQCDVNGCVMRMDNKDDHARNPGAPLAYEYRWNNDRWET
                     SGEYPYFCDRNDPSSAVKAQRSDYWIPNPDGSFFGERTLIIDGAGCPGEGPGTHLVPI
                     SLTPIDPPPPPSR"
     gene            2157..2612
                     /locus_tag="VFC01_27020"
     CDS             2157..2612
                     /locus_tag="VFC01_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HZN83302.1"
                     /translation="MTTSHASIDQHPDILALRASYDRAAESMTAQATFGLTLLTAIFV
                     ALLPHIVGFDTFTRLPASDLIVGITVAVMAMCFGSALDRTHGMTWTLPVFGVWIFFSP
                     WIFVASPTAGMIWSHMICGALITVCGLYAVYFGMRVRGFEGIRRPGSEH"
     gene            complement(2656..4356)
                     /gene="ilvD"
                     /locus_tag="VFC01_27025"
     CDS             complement(2656..4356)
                     /gene="ilvD"
                     /locus_tag="VFC01_27025"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZN83303.1"
                     /translation="MPSDSPDIKPRSRDVTDGLEKAAARGMLRAVGMGDEDFAKPQIG
                     VGSSWNEITPCNLSLDRLAKAVKDGVFAAGGYPMEFGTISVSDGISMGHEGMHFSLVS
                     REVIADSVETVMQAERLDGSVLLAGCDKSLPGMLMAAARLDLANVFLYAGSILPGIAK
                     LSDGTEKEVTIIDAFEAVGACSRGLMPREDVDAIERAICPGEGACGGMYTANTMASAA
                     EALGMSLPGSAAPPATDRRRDGFARRSGQAVVELLRRGITARDILTKEAFENAIAVVM
                     AFGGSTNAVLHLLAIAHEANVKLTLEDFTRVGARVPHLADVKPFGSHVMTDVDRIGGV
                     PVVMKALLDAGLLHGDCLTVTGQTMAQNLAHIAPPDPDGKVLRALTNPIHPTGGITIL
                     HGSLAPEGAVVKSAGFDSDVFEGTARVFERERAALDALEDGTITGGDVVVIRYEGPKG
                     GPGMREMLAITGAIKGAGLGKDVLLMTDGRFSGGTTGLCVGHVAPEAVDGGPIAFLHD
                     GDRIRLDVANGTLDVLVDPAEFESRKAGFEPLPPRYTTGVLAKYTKLVGSAAGGAVCG
                     "
     gene            4429..5031
                     /locus_tag="VFC01_27030"
     CDS             4429..5031
                     /locus_tag="VFC01_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HZN83304.1"
                     /translation="MTLTTRVAAVVAAVAAALFLSSCSNSTSNSNADHPKTDEPVITG
                     QPAGFNADDVAFATNMIPHHKQAVDLSDLVPDRSTNAELIALAQQISAAQQPEINVMK
                     VFLVQWNENPDTNSGHAGHGTTMQGMVDAATMTKLQSLNGAEFDKLWLESMIGHHQGA
                     IEMAKAEIANGDNVDAKSLAKNIVTTQEAEIGQMKQMLGG"
     gene            5033..5323
                     /locus_tag="VFC01_27035"
     CDS             5033..5323
                     /locus_tag="VFC01_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HZN83305.1"
                     /translation="MTAAHGYSQQKENYAKRLRRIEGQVRGIAKMIDEDKYCIDVLTQ
                     ISAVNSALQSVALGLLDEHLGHCVTQAVAEGGDEADAKLAEASAAIARLVRS"
     gene            complement(5401..5694)
                     /locus_tag="VFC01_27040"
     CDS             complement(5401..5694)
                     /locus_tag="VFC01_27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HZN83306.1"
                     /translation="MIVAFSVSPSGGDASMGAAVAEAIRVVRASGLPNETNAMFTNIE
                     GEWDEVMAVVKQAVDAVAAAAPRVSLVLKADIRPGYTGQLTGKVERIEEALGD"
     gene            5721..5942
                     /locus_tag="VFC01_27045"
     CDS             5721..5942
                     /locus_tag="VFC01_27045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83307.1"
                     /translation="MPDVRLLPATVEHLVALQTDPPAFARLIGSPVPDGWPQFPESID
                     FTIARLREHPDQAEWWMHFFLLPDTTLIG"
     gene            5952..6221
                     /locus_tag="VFC01_27050"
     CDS             5952..6221
                     /locus_tag="VFC01_27050"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN83308.1"
                     /translation="MGPPQDAVVEFGYEVAPAFRGKGLGVAVAKAMVDKATATGQVST
                     VIAHTLANENPSTGVLRHLGFAKRAQLVDPEDGPIWRWELPLTAC"
     gene            complement(6208..6597)
                     /locus_tag="VFC01_27055"
     CDS             complement(6208..6597)
                     /locus_tag="VFC01_27055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZN83309.1"
                     /translation="MTDIPSGYETLLERPLYGHLATTRPDGNAQVNPMWFDWDGEKLR
                     FTHTTKRQKYRNITGNPNVAMSISDPDNPYRYLEVRGVVEEVVPDPTGAFYLHLNDRY
                     SGPLKEAPPDKADRVIIVVRPTGFSKQ"
     gene            6693..7928
                     /locus_tag="VFC01_27060"
     CDS             6693..7928
                     /locus_tag="VFC01_27060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN83310.1"
                     /translation="MTVDTHTRPVNTWTPRVTVQLAVLAAAAFVYVTAEVVPVGALPA
                     IAADLRVSEALVGTLLASYALVAALTTVPLVRLTARWPRRTTLLLTLVCLTASQLISA
                     LAPNFTVLAGGRVLCALTHGLMWSVIAPIGARLVPATHAGRATTAVYVGTSLALVAGN
                     PMTAAMSELWGWRLAVVLVMVAAAIVTVAAWAVLPPMALPRDEAQPARRNARHHRNGR
                     LVTLTALTSIGVTGHFISYTFIVVIIRDVVGVHGPTLAWLLAAFGIAGLVSMGAMARP
                     LDRWPQASVVGCLAALALAFAVLTSLAIDHIAGLVALVVGVGAIVLWGAASTALPPML
                     QAAAMCTAPDDPDGASGVYVAGFQVGIMAGSLAGGLLYEHFGLALAIGASTALVMVAL
                     TGATATRGLFEIPQPTSRK"
     gene            8071..9120
                     /locus_tag="VFC01_27065"
     CDS             8071..9120
                     /locus_tag="VFC01_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZN83311.1"
                     /translation="MRGRMRGRLVTAIIAAGLVGGLALGNPSALADPEVPPPPAPIDP
                     AAAPAPPPDPFAPPPPADPLAVPPPADPAAPPAPPPFAPPNPFAPPAAQRMAIPEGTP
                     AGQNPTPYVGEPVFAPPTFNPTNGAKAGAAKPIYINFARPIADRAMAEQAIHISSVPP
                     VPGRFYWTSDTQVRWRPQDFWPAGTVVNIDASGTKSSFTVPEQLVATIDDATHQMTVM
                     RNGKPEKTIPVSMGMAAGGHTTPNGTYYVLEKFPTIVMDSSTYGVPVSSANGYKVTVQ
                     LAVRIDNSGNFVHSAPWSVGDQGKRNVSHGCINISPENAKWFYDNFGSGDPVVIKNSK
                     GIYNQPDGASDWQMF"
     gene            complement(9117..10106)
                     /locus_tag="VFC01_27070"
     CDS             complement(9117..10106)
                     /locus_tag="VFC01_27070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014360891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZN83312.1"
                     /translation="MQDIADHVGVSKALVSLVFRNAPGPSAETRKRVLAAADELGYRV
                     NRAAALMTARRSHLIGVMANIRSTFHAELVEDIVAEADKYGYEVVLGAVTPTHAEPAV
                     VETLLSFRCEAMILLGPELGDDAIAQLAQRAPVVVVGRRMSASTVDVVRTADGRGIGQ
                     VVDHLVELRHRRICHLSGGAGTIPADRKAGYVRAMKRHGLADGIDIIDGDFTERSGML
                     AAQELLSRRPRPTAVCAANDQSAIGLLDQLLRAGVDVPGDIAVAGYDDSMLARLAHID
                     MTTVSQEPRQQAERAVRAVVERLDESRHERVDVLLEPRLVVRHTTVPQLRQPA"
     gene            10235..11410
                     /locus_tag="VFC01_27075"
     CDS             10235..11410
                     /locus_tag="VFC01_27075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006370657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN83313.1"
                     /translation="MTSSLGVAVIGGGMAGRAHAAGYRTATTLFGTERPDVRLVAIAD
                     TNAAVADDTAKRYGYERAEYDWQAIADAPDIDAVSVVVANHLHREIVEGLLAGGKHVL
                     CEKPLAGSLADAEAMVAAAERSDRVTAIGYTYRRVPAVEQIRRELAAGTLGELAHFDG
                     HYWCDYALDPTAPITWRYRGGPGTGALADVGSHLIDMSEFLCGPIAEVSGAVFTTLIE
                     QRPVPLGVTYGHTKDAVSDELAPVENEDVVSFTARFEDGAIGTFSASRVAHSLPDELS
                     FNVFASRGSAAFDLQRPGEFQISTDNIRSDVAGPRRVLIGPDHPYIRGGLPMDAAGVG
                     HGVADMFGWQLRAFLDQIAGVGELGPLPGFDAGMHGMRVVAAITESAQSGGAAVKVV"
     gene            11429..12427
                     /locus_tag="VFC01_27080"
     CDS             11429..12427
                     /locus_tag="VFC01_27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZN83314.1"
                     /translation="MKLGVYTAILHDKPLREALEVIASLGLTGAEINAGGFLPTPHLP
                     VDDLLTGAVTPREYLANFDGTGVEIAGLNCNGNPLHPDLEVGPEDADDLRKAIRVAGL
                     LGVDRVVTMSGLPQAHPGGRWPAWHVNTWDSGYLDSLDYQWDEVALPFWTEIDALARQ
                     NDVKVAIEMHPQNLVFNPPTLRRLVERIGATNVGAEMDPSHLFWQGIDPVEAIDWLGP
                     LVVHAAAKDTRINENCKIYGVLDERFTRIPLSENPTGLGGRHVVNKWPEDSAWDFVAV
                     GRGHDTEFWARFLQALERVDPDMAVNIEHEDVELGQLEGLQVAAETLKAANSAAVG"
     gene            12441..13424
                     /locus_tag="VFC01_27085"
     CDS             12441..13424
                     /locus_tag="VFC01_27085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZN83315.1"
                     /translation="MKSIRITAACVGVLVALTACSSTGGKPRTTGDGMGAGQADTPRF
                     TVAMVTHGPPGDTFWDLIRKGAETAAKKDNIELKYSAELQGPDQANLVQNAIDSKVNA
                     IAVTLARPEALKAAVQSATEAGIPVVALNAGISDWKAAGAKAYFGQDETISGEAAGKR
                     LASEGAKKVICVIQDQGNVALESRCAGVKSGFGGAVENLNVNGTDMPSVQSTITAKLQ
                     QDPAIDHIVTLGAPIALAAAESAATAGSSAKIVTFDTNAALVDAIKDGKVGWAIDQQP
                     FLQGYLAIDSLWLYLNNGNVIGGGQPTLTGPAFIDKSNIDAIADYAKNGTR"
     gene            complement(13426..15402)
                     /locus_tag="VFC01_27090"
     CDS             complement(13426..15402)
                     /locus_tag="VFC01_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HZN83316.1"
                     /translation="MTVEAIRSGIDLSHVDSDARPQDDLFGHVNGRWLAEYKIPADRA
                     TDGAFRSLYDRAEEQVRDLITEAAASGAGKGTDEQRIGDLYASFMDEQTVAGRGVQPL
                     LDELAAIDAAETPEALAAVVGALQRTGVGGGTGAYVDTDSKDSTRYLVHLSQSGLGLP
                     DESYYRDEQHAEILAAYPQHIAAMFALVYGGDHTETAARIVALETKLAAAHWDVVKRR
                     DADLTYNLRTFADLPSEAPGFDWSGWVRALGTTPEGVAEVVVRQPDYLTAFAAAWSGE
                     DFEDWKNWARWRLIHARAALLTDDLVAEDFAFYGRRLSGTEEIRDRWKRAVSVVESLM
                     GDAVGKLYVERHFPPGAKARMDELVANLREAYRVSINDLEWMTPATREKALAKLDKFT
                     PKIGYPAKWRDYSKVVIERDDLYGNYRRGYEVNSDRELAKLGGPVDRDEWFMTPQTVN
                     AYYNPGMNEIVFPAAILQPPFFDADADDAANYGGIGAVIGHEIGHGFDDQGAKYDGDG
                     NLVDWWTDEDRTEFGARTKALIEQYEEFTPRQLGDNHHVNGAFTVGENIGDLGGLSIA
                     LLAYRLSLKGKDAPVIDGLTGEQRVFYGWAQVWRTKSRDAEAIRRLAIDPHSPPEFRC
                     NGVIRNMDAFYDAFEVSEDDELYLDPQRRVRIWN"
BASE COUNT         2684 a         5242 c         5083 g         2638 t
ORIGIN      
        1 cgccgcccac tatgaacgct ccgacgacgc cgccgccatc gcggtgcctg accgccgctc
       61 cacgtgggcg ctgcgcgaat tggtggtgtt gcacgagatt gcccaccacc tgagcgacgc
      121 ccagcctccg cacggcccgg agttcgtcgc gacattctgt gaactcgcag aagcggtaat
      181 ggggccggag gtcgcccatg tcttgcgggt ggtctacgcc aaagaaggtg tccggtagcg
      241 tttgtcgcgt ggcagatccg gatccgaagg cactcgacga gctgttcacc cgggtgctgg
      301 acacggagga cgacgcactg cgagcggcgc gggaagcggg cgttgccgcc gatatgcctg
      361 cgatcgaggt gtccgcccaa cacggaaagc tgttgtttct cctcgtgacg gcgatgcgcg
      421 ccacccgcgt gctcgagatc ggcacgctcg cgggatacag caccatccag ctggcccgcg
      481 ccgtcgggcc gggcggccgg gtcatcacgc tggagtacga gccgaagcat gccgaggtgg
      541 cgcgggagaa cctggcgcgc gcgggcgtcg acgaccgggt ggaggtgatc gtcggcgtcg
      601 cgctcgacac gctgccgacg ctggccggcc gcgacgagac gttcgatctg atcttcatcg
      661 acgccgacaa ggagaacaac gtcgcctatg tcgagtgggc gatcaaactc ggcgcgccgg
      721 gctcaatcat cgtggtggac aacatcgctc gcttcggacg cgtgctcgac cccgcgccgg
      781 acgacctgca ggcacgcgct gtgcgcgaca tgctggagat gatgggcgcg aacccgcggc
      841 tggaggcggc ggcgattcag acggtcggca ccaagggctg ggacggtttc gcggtggcga
      901 tcgtcaaaga gcctggcgaa taagcctggt actgacagaa cccctcacgg ataggggtgc
      961 ggacgtagcg gttatgtcat ggacaaccgc aacgacatcc gtgagttcct gagactcccc
     1021 gtccgatgac gggctcaaac tgcttgcgag ctgggctgtc cgtcaccccc tgagccagca
     1081 gcacgcaatg gccacgccgg catccggcga ccaccacgag gaaaaccaga tgcggatgct
     1141 cgaccgctag ccggtcagcg cgaccagatt gaccgatgcg gcgttcctgc ccgccatctt
     1201 cgatcgattt gcccgcacct gacgcgctgt tgtgttatcc aggccgcatg atgaaatttg
     1261 cgctgggtgc cgtcttagct gccagctccg cactggcggt gtctcccgtc gctcatgctg
     1321 atcgcgctga accaccgccg ctctacgggt actacaacgt gtccatcgac ttctcgaagc
     1381 agacgttcaa cggtatgccg acaccgatga attcgataac ggtgcctgtc gaattcacca
     1441 cccaatgtga tgtgaacggt tgtgtcatgc gcatggacaa caaggacgac catgcccgca
     1501 atccaggcgc tcccttggct tacgagtaca gatggaacaa cgaccgttgg gaaacgagcg
     1561 gtgagtatcc ctacttctgt gatcgcaatg accccagcag cgccgtgaag gcccaacgct
     1621 ccgactactg gatcccgaac cctgacggca gcttcttcgg agagcgcacc ctcatcatcg
     1681 atggagccgg atgcccaggt gagggaccag gcacccattt ggtgccgata tccctgacac
     1741 ccatcgatcc accaccgcca ccttcgcggt agccatcggt cagcgtctgg ataactgggc
     1801 taaccgcaga ccgcaccgcc cgtcgcggtc tgcgcgcggc cagggtccag cgacatcccg
     1861 gtcgacgtcg gctggcgcgt ccaccatgtg cggccgacac ccgacggcgc cgagattgtg
     1921 gccacggttc tggatgggcg gccgccatgt cgcggtgtgg tcgggaacgt cgttggcgga
     1981 cagggcgctt cgaccaatcg ctggccgtct cccgccgctc tacgcgcgct tcggcaacgc
     2041 ctgatctagc gcccttgctt gacggatccc gataccgcaa acgagcgtag tcttgatgtt
     2101 gatggatggg gtgcggtgag cccaaaccgc ttgccgtgta acggaaagga tacgaaatga
     2161 caacttccca tgcatcaatc gatcagcatc ctgacatatt ggctctgcgg gcgagctatg
     2221 accgcgccgc cgagtcgatg accgcgcagg ccaccttcgg cctgacgttg ctgacggcga
     2281 tatttgttgc cctattaccg catatcgtcg gcttcgacac cttcacgcgg ctacctgcca
     2341 gcgatctgat cgtaggaata accgttgctg tcatggcaat gtgcttcggc tcggcactgg
     2401 accgtaccca cggcatgacg tggacgttgc cggtcttcgg tgtgtggatc ttcttctcac
     2461 cgtggatctt tgtcgcgtca ccgaccgcgg gcatgatctg gtcacacatg atctgcggtg
     2521 cgctgatcac cgtgtgcggc ctgtatgccg tgtacttcgg aatgcgcgtg cgcggtttcg
     2581 aaggcatacg taggcccggt tccgaacact agtcagacac gacgagaccg gccggggcga
     2641 tcccggccgg ccgtcctagc cgcagaccgc gccgccggcg gccgacccga ccagcttggt
     2701 gtacttggcc agtacgccgg tggtgtagcg cggcggcagc ggctcgaaac ctgccttgcg
     2761 cgactcgaat tccgccggat cgacgagcac gtccagcgtg ccgttggcca cgtcgagccg
     2821 gatccggtca ccgtcgtgca ggaacgcgat cggtccaccg tcgacggcct ctggcgcgac
     2881 gtggcccacg cacagacccg tcgtgccgcc cgagaaccgg ccgtcggtca tcagcagcac
     2941 atccttgccg aggcctgcgc ctttgatcgc gccggtgatg gcgagcatct cgcgcatgcc
     3001 gggtccaccc ttggggccct cgtagcggat cacaacgacg tcgccaccgg tgatcgtgcc
     3061 gtcctccaga gcatccaacg cggcccgttc gcgttcgaaa acccttgcag tgccctcgaa
     3121 cacgtcggag tcgaagcctg ccgacttcac cacagcgccc tccggcgcca gcgacccgtg
     3181 cagaatcgtg ataccgccgg tcggatggat cgggttcgtc aatgcccgca gtaccttgcc
     3241 gtccggatcc ggcggcgcga tgtgtgcaag attctgcgcc atcgtctgcc cggtgaccgt
     3301 caggcaatcc ccgtgcagca gaccggcatc caggagcgcc ttcatcacga cgggcacacc
     3361 gccgatgcgg tcgacgtccg tcatcacgtg gctgccaaag ggtttcacgt cagcgaggtg
     3421 ggggacgcgc gcgcccacgc gggtgaagtc ctccagcgtc agcttgacgt tggcttcgtg
     3481 tgcgatggcc agcaggtgca gcaccgcgtt ggtcgacccg ccgaacgcca tcaccaccgc
     3541 gatcgcgttc tcgaacgctt ccttggtgag gatgtcgcga gcggtgatgc cgcggcgcag
     3601 gagttcgacg acggcctggc cgctgcggcg ggcgaacccg tcgcgcctgc gatcggtggc
     3661 gggcggtgcg gctgacccgg gcagcgacat acccaaagct tctgccgccg acgccatcgt
     3721 gttggcggtg tacatgccgc cgcaggcgcc ctcgccgggg cagatcgcgc gctcgatcgc
     3781 gtcgacgtcc tcccggggca tcaaacctcg cgagcatgca ccgaccgcct cgaacgcgtc
     3841 gatgatcgtc acttccttct cggtgccgtc ggacagcttg gcgatgccgg gaaggattga
     3901 gccggcgtag aggaaaacgt tggccaggtc gagacgcgct gcggccatca gcatgccggg
     3961 cagcgacttg tcgcaaccgg ccagcagtac cgaaccgtca aggcgctcgg cctgcatgac
     4021 cgtctcgacg ctgtcggcga tcacctcacg ggagacgagc gagaagtgca tgccctcgtg
     4081 gcccatcgag atgccgtcgg acaccgagat ggtgccgaac tccatcggat agccgcccgc
     4141 cgcgaacact ccgtccttga cggccttggc cagccggtcc agcgacaggt tgcagggggt
     4201 gatctcgttc catgacgagc cgacgccgat ctgcggtttg gcgaagtcct cgtcgcccat
     4261 gcctactgcc cgcaacatcc cgcgggcagc ggccttctcc aggccgtcgg tgacgtcgcg
     4321 gctgcggggc tttatatcgg gggagtctga gggcatgcgt ttcagtatgc cgtcggcgtt
     4381 tcgacaggcc aaaccgttat gaataccccc acggggtacc ctgtgagcat gacgctgacc
     4441 actcgagttg ccgcagtggt ggccgcagtg gccgccgcac tgttcctgtc gtcgtgtagc
     4501 aactcgacgt ccaacagcaa cgccgaccac ccgaagaccg acgagcccgt catcaccggc
     4561 cagccggccg gcttcaacgc cgacgatgtc gcgttcgcca ccaacatgat tccgcaccac
     4621 aagcaggccg tcgacctgtc ggacctggta cccgaccgct cgacgaacgc cgaacttatc
     4681 gcgctggccc aacagatctc cgctgcgcag caaccggaga tcaacgtcat gaaggtgttc
     4741 ctggtgcagt ggaacgagaa ccccgacacc aactccggtc acgccgggca cggcaccacc
     4801 atgcagggca tggtcgacgc ggcgacgatg acgaagttgc agtcgctgaa cggcgcggag
     4861 ttcgacaaac tatggctgga gtcgatgatc ggccaccacc agggagcgat cgaaatggcc
     4921 aaggccgaga tcgccaacgg cgacaatgtg gatgccaagt cgctggccaa gaacatcgtc
     4981 accacgcagg aagccgagat cggccagatg aagcagatgc tgggaggctg aaatgactgc
     5041 cgcacacggt tattcgcagc agaaagagaa ttacgccaag cggctgcgcc gaatcgaggg
     5101 ccaagtccgc ggcatcgcga agatgatcga cgaggacaag tactgcatcg acgtgctcac
     5161 ccagatcagc gcggtcaaca gtgcgctgca gtcggtggcg ctcggcctgc tcgacgagca
     5221 cctcggccat tgcgtcacgc aggccgttgc cgaaggtggc gacgaggccg acgccaaact
     5281 ggccgaggcg tcagcggcca tcgcccgatt ggtgcgctcc tagtattccc gggcgaacgt
     5341 tccgtaccgc tcgaaatttc gcccaaaaat cgagcggtaa ggaacgttcg cgccaccggt
     5401 ctagtcgccc aacgcctctt cgatccgttc caccttgccg gtgagctggc cggtatagcc
     5461 cgggcggatg tcggccttga gcaccaggct cacccggggc gcggcagcgg caaccgcgtc
     5521 cacggcttgc ttgacaaccg ccatcacctc gtcccattca ccttcgatgt tggtgaacat
     5581 cgcattggtc tcattgggca agcccgacgc gcgcaccacc cgaatcgcct ccgccaccgc
     5641 ggcgcccatg ctcgcgtcgc cgccggacgg actcacactg aaggccacaa tcattcgatc
     5701 agcctgccac cggaggatcc atgccagacg tccgcctgct gcccgcgacg gtcgagcacc
     5761 tcgtcgcatt gcagaccgat ccgccagcat tcgcgcggtt gatcggcagc ccggtgccag
     5821 acggctggcc gcagtttccg gagtccatcg acttcacgat cgcgcgactc cgcgagcatc
     5881 ccgaccaggc cgagtggtgg atgcatttct tcctgctgcc cgacaccacg ctgatcggtt
     5941 aggggggcta tgtgggtccg ccgcaggacg ccgtcgtcga attcggctac gaggtggcgc
     6001 ccgcgttccg cggcaagggg ctcggcgtgg ccgtcgccaa ggcgatggtc gacaaggcaa
     6061 ccgccaccgg ccaggtgtcg acggtcatcg cccacactct ggccaacgag aacccctcga
     6121 cgggcgtgct tcgccacctc ggcttcgcca agcgcgccca actcgtcgac cccgaagacg
     6181 ggccgatctg gcgctgggag ctgccgctta ctgcttgctg aatccagttg gccgcacgac
     6241 gatgatcacc cggtcggcct tgtcgggtgg tgcctccttc aacggaccgc tgtagcggtc
     6301 gttgagatgc agatagaacg cgccggtggg gtcgggcacg acctcctcga ccacgcctcg
     6361 cacctccaga tagcgatacg ggttatccgg atcgctgatc gacatcgcca cattcgggtt
     6421 accggtgatg ttgcggtact tctgccgctt ggtggtgtgg gtgaagcgaa gcttctcccc
     6481 gtcccagtcg aaccacatcg gattcacctg ggcattgccg tcgggcctcg tcgtggcgag
     6541 gtgcccgtac agcggacgct ccaacaacgt ttcatatccc gaagggatgt cggtcatcac
     6601 gatcctctct cgaataggcg gcgatccact accaacgcac aacccgctct agcgcaaatc
     6661 ccatcctcag tcgatacggt ggtgctcccg ccatgactgt cgatacccac acacgacccg
     6721 tcaacacgtg gacgccgcgc gtcaccgtgc agctggccgt gctcgccgcc gccgcgttcg
     6781 tctacgtgac ggccgaggtc gtaccggtgg gagcgctgcc tgcgatcgcc gccgatcttc
     6841 gggtcagtga ggcgctggtg ggcacactgc tggcgagcta cgcgctcgtc gcagccctca
     6901 ccaccgttcc gctggtccgc ctcaccgccc gatggccacg acgcaccacg ctcctgctga
     6961 cattggtgtg tttgactgcg tcacaactga tttcggctct cgcacccaac ttcaccgtgc
     7021 tggccggtgg ccgggtgttg tgcgcgctca cgcacgggct gatgtggtcg gtgatcgcgc
     7081 cgatcggcgc ccgactggtg cctgcaacac acgctggccg cgccaccact gcggtgtacg
     7141 tcggtaccag cctcgcgctg gtcgctggaa acccgatgac cgcagcgatg agcgaactat
     7201 ggggctggcg gctggctgtg gtgctggtga tggtggccgc tgcgatcgtc acggtggccg
     7261 cgtgggccgt tctgccgccg atggcactgc cacgtgacga ggcacagccc gcccgccgca
     7321 acgcacgcca tcaccgcaac ggtcggctgg tcacgctgac cgcgttgacg tcgatcgggg
     7381 tcaccgggca cttcatctcc tacacgttca tcgtcgtgat catccgtgac gtcgtcggcg
     7441 tgcacggccc gacgctggcc tggctattgg ccgcgttcgg catcgccggc ctggtctcga
     7501 tgggtgcaat ggcccgaccg ctggatcggt ggccacaagc ctcggtggtc ggctgcctcg
     7561 ccgcgctggc attggcgttc gcggtgttga cgtcgctggc gatcgaccac atcgccgggt
     7621 tggtcgccct ggtggtcggt gtgggtgcga tcgtgctgtg gggtgcggcc tcgacggcac
     7681 tgccgccgat gctgcaggcg gccgccatgt gcaccgcgcc cgatgatcca gacggcgcgt
     7741 cgggcgtgta tgtcgccggc ttccaggtcg gcatcatggc aggttcgctg gcgggcggac
     7801 tgctctatga gcacttcggg ctcgcgctgg cgatcggcgc gtcgacggct cttgtgatgg
     7861 tggcgctgac gggtgcgacc gccacccgcg gcctgttcga gattcctcag ccaaccagca
     7921 ggaagtaaca ttgcgcgctg cgtcgcgatg aacaccgcag gtcagcgacg tgagctgggc
     7981 agtcgcccgg catcaggcag caccctcgac gcggtagctg gtcttttcgt tgtgcgagac
     8041 tggtcgagat tcgtaactgg aggtggatgt atgcgagggc ggatgagagg caggctggtc
     8101 accgcgatca tcgcggccgg actggttgga ggactggcgc taggaaaccc atctgcgctg
     8161 gctgaccccg aagttccgcc gccaccggca ccgatcgatc cggctgcagc gcccgcgcca
     8221 ccgcctgatc cgttcgcccc gccgccgccg gccgatccgc tcgcggtgcc accgcctgcc
     8281 gatccggccg ccccgccggc cccgccgccg ttcgcaccgc cgaacccgtt cgcgccgccg
     8341 gccgcccaac ggatggcgat tcccgagggc acgccggccg gccagaaccc gacgccgtac
     8401 gtcggcgaac cggtgttcgc gccgccgacg ttcaacccga cgaacggcgc caaggccgga
     8461 gccgcgaagc cgatctacat caacttcgcg cggccgatcg cggaccgcgc gatggctgag
     8521 caagcgatcc acatctcgtc ggtcccgccg gtgcccggcc gcttctactg gaccagcgac
     8581 acccaggtgc gctggcggcc gcaggacttc tggccggccg gcaccgtcgt caacatcgac
     8641 gcgagcggca ccaagtcgag cttcaccgtt ccggagcagc tcgtggccac cattgatgac
     8701 gcgacgcatc agatgacggt catgcgcaac gggaagccgg agaagaccat ccctgtgtcg
     8761 atgggtatgg cggctggcgg ccatacgacc ccgaatggca cgtattacgt gctcgagaaa
     8821 ttccccacca tcgtcatgga ctcctcgacg tacggcgtgc ctgtcagttc agccaacggc
     8881 tacaaggtca ccgtgcagct cgcggttcgg atcgacaaca gcggcaactt cgtgcacagc
     8941 gcgccgtggt cggttggtga ccagggcaag cggaacgtga gccacggctg catcaacatc
     9001 agccccgaaa atgcgaagtg gttctacgac aacttcggca gcggcgatcc ggtcgtgatc
     9061 aagaactcga agggcatcta caaccagccg gacggcgcgt ccgactggca gatgttctag
     9121 gccggttgtc gtagctgcgg cacggtggtg tgccgtacca cgagccttgg ctcgagcagg
     9181 acgtcgacgc gttcgtgccg gctctcatcg agtcgttcca cgactgctcg gacggctcgt
     9241 tcagcctgct gtcgcggttc ctggctgaca gtggtcatgt cgatgtgcgc gagcctggcc
     9301 agcatgctgt cgtcgtagcc ggccaccgcg atgtcgccgg gcacgtcgac gccggcccgc
     9361 aacagctggt cgaggagtcc gatcgcgctt tgatcgttgg cggcacacac ggcagtcggg
     9421 cgtggtcgtc ggcttaacag ctcctgcgcg gcgagcatgc ccgagcgctc ggtgaagtcg
     9481 ccgtcgatga tgtcgatgcc gtcggccagc ccgtgtctct tcatcgcccg cacgtaaccc
     9541 gctttgcgat cggccgggat cgttcccgcc ccgccactga gatggcagat ccgccggtgc
     9601 cgcagttcga cgaggtggtc caccacctgt ccgatgccgc ggccgtccgc ggtgcgcacg
     9661 acgtcgacgg tgctggcgga catgcgccgc ccgacaacga caaccggtgc tcgttgtgcc
     9721 aactgtgcga tcgcgtcgtc gccgagctcg gggccgagca ggatcattgc ttcacaccga
     9781 aagctgagca gggtttcgac gacggcgggt tcggcgtgcg ttggtgtgac ggcgccgagc
     9841 acgacctcat acccgtattt gtcggcctcg gcgacgatgt cctcgacgag ttcggcgtga
     9901 aaagtactgc gaatattggc cataacgccg atgaggtgtg accgccgggc agtcatcagt
     9961 gctgccgccc ggttgacgcg gtagccgagt tcgtccgcgg ctgcaagcac acgcttgcgg
    10021 gtctccgcac tcgggccggg cgcgttgcgg aacacgagtg agacgagggc cttcgagacg
    10081 ccgacgtgat cggcgatgtc ctgcatcgtc ggccggtcgc ggtgattggg cacggccctc
    10141 ctcttgacaa cggactgtga cggcgctcat agtagcggaa ctagaccggt ctaatagatc
    10201 ggtttaagca caagacttca tgggaggctt cgacgtgacg agctcattag gcgtggccgt
    10261 catcggtggc ggcatggccg gccgggcaca tgccgcggga taccggaccg cgaccacgct
    10321 gttcgggacc gagaggccgg atgtgcggct ggtcgcgatc gccgacacca acgccgccgt
    10381 cgccgacgac accgcgaagc gatacggcta cgagcgggcc gagtacgact ggcaggcgat
    10441 cgccgacgca cccgatatcg acgccgtaag cgtggtcgtg gccaaccacc tacaccgcga
    10501 aatcgtcgag gggctactgg ccgggggcaa gcacgtgctc tgcgaaaagc cgctggccgg
    10561 ttcactggcc gatgccgagg cgatggtcgc ggcggccgaa cgcagtgacc gcgtcaccgc
    10621 catcggctac acgtaccgac gggtgcctgc ggtcgaacag atccggcgcg aactggccgc
    10681 gggcacgctc ggcgagttgg cgcacttcga cggccactac tggtgcgact acgcgttgga
    10741 cccgaccgcg ccgatcacct ggcgctatcg cggcggaccc gggaccggcg cgctagccga
    10801 cgtcggcagc cacctgatcg acatgtcgga atttctgtgc ggtccgatcg cggaagtcag
    10861 cggcgcggtg ttcaccacgc tgatcgagca acggcccgtg ccgcttggag tcacctacgg
    10921 ccacaccaag gatgcggtca gcgacgaact cgcgcccgtc gaaaacgaag acgtcgtcag
    10981 cttcaccgcc aggttcgaag acggtgcgat cggcaccttc tcggcctctc gcgtcgcgca
    11041 cagcctgccc gacgaactca gtttcaacgt gttcgcgtcg agaggctcgg cggcattcga
    11101 cttgcagcgc cccggcgaat tccagatctc gaccgacaac atccgcagtg acgtcgccgg
    11161 gccacgccgg gtgctcatcg ggcccgatca cccctacatc cgcggcgggc tgccgatgga
    11221 cgccgccggg gtcggccacg gggtggccga catgttcggc tggcagctcc gcgcgttcct
    11281 cgaccagatc gcgggcgtcg gcgagctcgg cccgctgccg ggattcgacg cgggcatgca
    11341 cggcatgcgg gtcgtcgccg cgatcaccga atccgcacag tccggcggcg ctgccgtcaa
    11401 agtcgtctaa ccagaacgga gttcacgcat gaagctcggt gtctacaccg ccatcctgca
    11461 cgacaagcct ttgcgcgaag ccctcgaagt catcgcgtcg ctgggactga ccggcgcgga
    11521 gatcaacgcg ggcgggttcc tgccgacgcc acacctgccg gtcgacgatc tgctgaccgg
    11581 tgccgtcaca ccgcgggagt atctcgccaa cttcgacggc accggtgtcg agatcgcggg
    11641 gctcaactgc aacggcaacc cactgcaccc ggacctcgag gtcggaccgg aagacgccga
    11701 cgatctgcgc aaggcgatcc gcgtggccgg tctgctcggc gttgatcggg tggtgacgat
    11761 gtcgggcctg ccccaggctc accccggcgg acggtggccc gcgtggcacg taaacacttg
    11821 ggactcaggc tatctcgatt ccctcgacta tcagtgggat gaggttgcgc tgccgttctg
    11881 gaccgagatc gacgcgctgg cccgccagaa cgatgtcaag gtggccatcg agatgcaccc
    11941 acagaatctg gtgttcaacc cgccgacgct gcgacgtctg gtcgagcgca tcggcgccac
    12001 gaacgtcggc gcggagatgg acccctctca tctgttctgg caggggatcg acccggtcga
    12061 ggccatcgac tggctgggac cgctggtggt gcacgccgcc gcgaaggaca cccggatcaa
    12121 cgagaactgc aagatctacg gtgtgctcga cgagcggttc acccgaatcc cgttgagcga
    12181 gaacccgacc gggctcggcg gcaggcatgt ggtgaacaag tggccggaag actcggcgtg
    12241 ggacttcgtc gcagtcggcc gcggccatga cacggagttc tgggcgcggt tcctgcaggc
    12301 gctggagcgc gtcgacccgg acatggcagt gaacatcgag cacgaagacg tcgagctcgg
    12361 ccagctcgaa ggactgcaag tcgccgccga gacgctcaag gccgccaact ccgcggctgt
    12421 cggctaaagg agatgtaatg atgaagtcca tacgaatcac agcggcatgt gtcggcgttc
    12481 tcgtcgcttt gacggcctgc tcgagtaccg gcggtaaacc ccgcacgacc ggcgacggca
    12541 tgggcgccgg ccaagccgac accccgcggt tcaccgtcgc catggtcacc cacggaccgc
    12601 cgggcgacac gttctgggac ctgatccgca agggcgccga aaccgccgcg aagaaggaca
    12661 acatcgagct gaagtactca gccgaactcc aaggacccga ccaggccaac ctcgtgcaga
    12721 acgcgatcga cagcaaggtc aacgcgatcg cggtgacgct cgcacgaccc gaggcgctga
    12781 aggccgcggt gcaatcagcc acggaggccg gaattccggt ggtggctctc aacgccggca
    12841 ttagcgactg gaaggccgcc ggcgcgaagg cctatttcgg ccaggacgag acgatctcgg
    12901 gcgaggccgc gggcaagcgg ctcgcgagtg agggcgccaa aaaggtcatc tgcgtcatcc
    12961 aggaccaggg caacgtcgcg ctggaatcgc gttgtgcggg cgtcaagtcc ggcttcggcg
    13021 gagcggtgga gaacctgaac gtcaacggca ccgacatgcc gtccgtccaa tccaccatca
    13081 cggcgaagtt gcagcaggac ccggccatcg atcacatcgt caccctgggt gcgccgatcg
    13141 cgctggcggc tgcggaatcg gccgcaaccg caggcagcag cgccaagatc gtcaccttcg
    13201 acaccaacgc ggccctggtc gacgcgatca aggacggcaa ggtgggctgg gccatcgacc
    13261 agcagccgtt cctgcagggc tatctcgcga tcgactcgct gtggctgtac ctgaacaacg
    13321 gcaacgtcat tggcggtgga cagcccacac tgaccggccc agcgttcatc gacaagtcca
    13381 acatcgacgc catcgccgac tacgcaaaga acggaacccg ctgagctagt tccagatgcg
    13441 aacgcggcgc tgtgggtcga gatatagctc gtcgtcctcg ctgacctcga atgcgtcgta
    13501 gaacgcatcc atgttgcgga tgacgccgtt gcaccggaac tccggcggcg agtgcggatc
    13561 gattgccaac cggcgaatgg cttcggcgtc acgggatttg gtgcgccaca cctgcgccca
    13621 gccgtagaac acgcgctgct cgccggttaa cccgtcgatc accggcgcgt ccttgccctt
    13681 cagtgacagc cggtaggcca gcaaggcgat cgacaacccg ccgaggtcgc cgatgttctc
    13741 accgaccgtg aaggcaccgt tcacgtggtg gttgtcgccg agctggcggg gcgtgaactc
    13801 ctcgtactgc tcgatcaacg cctttgtccg ggcaccgaat tcggtccggt cctcgtcggt
    13861 ccaccagtcc accaggttgc cgtcgccgtc gtacttggcg ccttggtcgt cgaacccatg
    13921 gccgatctcg tggccgatca ccgcgccgat accaccgtag ttcgcggcgt cgtcggcatc
    13981 ggcgtcgaag aacggcggct gcagaatcgc tgcggggaag acgatttcgt tcatgcccgg
    14041 gttgtagtag gcgttgacgg tctgcggcgt catgaaccac tcgtcgcgat ccaccgggcc
    14101 gccgagtttg gccagctcgc ggtcggagtt cacctcatag ccgcggcggt agttgccgta
    14161 caggtcgtcg cgctcgataa cgaccttcga gtagtcgcgc catttggcgg gatagccgat
    14221 cttcggggtg aacttgtcga gcttggccag cgccttctcg cgcgtggccg gtgtcatcca
    14281 ctccaggtcg ttgatgctga cgcggtatgc ctcacgcaga ttggcgacca gttcgtccat
    14341 gcgagccttc gcgccgggcg gaaaatgccg ctcgacgtag agtttgccga ccgcatcgcc
    14401 catcaggctc tctaccacgg acacggcgcg cttccagcgg tcgcggatct cctcggtgcc
    14461 gctcaaccgg cggccgtaga acgcgaagtc ctcggccacc aagtcatcgg tgagcagggc
    14521 tgcgcgcgca tggatgagcc gccagcgggc ccagttcttc caatcctcga agtcctcgcc
    14581 cgaccaggcc gcagcgaagg cggtcaggta atcaggttga cgtacaacga cttccgcgac
    14641 tccctcgggc gtcgtgccca gcgctctgac ccagccagac cagtcgaagc ccggcgcctc
    14701 agagggcagg tcggcgaacg tgcgcaggtt gtaggtcagg tcggcgtcgc ggcgcttgac
    14761 gacgtcccag tgcgcggccg ccagcttggt ctccagtgcg acgatccgcg cggccgtctc
    14821 ggtgtggtca ccgccgtaga cgagcgcgaa catcgcggcg atgtgctgcg gataggcggc
    14881 gaggatctcg gcgtgctgct cgtcgcggta gtagctctcg tcgggcagcc cgaggccgga
    14941 ctggctcagg tgcaccaggt agcgcgtcga gtctttggag tcggtgtcga catacgcgcc
    15001 ggtgccgccg ccgacgccag tgcgctgcag agcgcccacg accgccgcca gggcctcggg
    15061 ggtctccgcg gcgtcgatcg cagccagttc gtcgagcagc ggctgcacac cacgtccggc
    15121 cacggtctgc tcgtccatga agctcgcgta caggtcaccg atgcgctgct cgtccgtccc
    15181 tttgccagcg cctgacgccg ccgcctccgt aatcaagtcc ctgacctgct cctcggcgcg
    15241 gtcgtatagc gaccggaagg cgccgtcggt cgcgcggtcg gccggaatct tgtactcggc
    15301 caaccagcgg ccgttgacat ggccgaagag atcgtcttgg gggcgggcat cggagtcgac
    15361 gtgggaaaga tcgataccgg aacgtatggc ttctaccgtc accccaacag ggtgccagaa
    15421 gtcgtcgggg cggtactggc ggcccggcgg cacacccggc cacggagggg ctgccggaga
    15481 ctcgccgggt agcctctcgg ccatgcccga cgacgagccc acgccggacc ccgacggcgg
    15541 tcgcgtcttc accggcttcg gcatcgcctc ggcggccctc ggcgcgctcg cggtcgcggc
    15601 cgtcgtcgtg gccggactca tctggactca gcaccgcggc gacaccg
//
LOCUS       DASRJA010000610        13145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3455610,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000610 DASRJA010000000
VERSION     DASRJA010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3455610"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VFC01_27095"
     CDS             complement(<1..905)
                     /locus_tag="VFC01_27095"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HZN83317.1"
                     /translation="MLCGLLVGGIITALELGGQDAKPDVRNDREAGLQAAPVIGSAQS
                     GDCLNWPANAPGQPSFVQCQDDHLFEVADSFDMRNPAAPCQLAVQRYLGSHYDPNGKF
                     TLTALWPDKVAETHPEGRRILCGLQLLGPDGQPVPFKGQVAQLDQSKVWPTGTCLGID
                     PANNQPTDTPVDCAAPHAVEVTGTANLDESFGGRLPAQGEQDAFLEEACARMADEYLA
                     PSSLMATGLTVHYTPVSPAGWLAGSRQVSCGIGATLSDNQGWATLTGTGRGGQLVSGL
                     PKAMPAVAPEIEENTTDLYLDTPTTV"
     gene            complement(1023..1961)
                     /locus_tag="VFC01_27100"
                     /pseudo
     CDS             complement(1023..1961)
                     /locus_tag="VFC01_27100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            3187..4845
                     /locus_tag="VFC01_27105"
     CDS             3187..4845
                     /locus_tag="VFC01_27105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83318.1"
                     /translation="MPNPLLAAVVETSTRSSCEAVDARVDGWCAGAFPSLFTPVAGVL
                     GTGLDIWDRTVGLGASVVAGTAITAGLIGVGLDTALAEALLTPLEVLVEGGVLPAQLA
                     TSIAAMVGGFTTGVNIGIAGVTTGIAWKAALDAALANTIAAAGADIINALIAGFPNMD
                     FPAAIAAVIGGLAAMGETALTWLDATVTLGASVVAGIAMTAATSVVELNTALTGAFLE
                     CLEALAESGVLPASAATALAGLVGSVSSAANAGILGLGSAVAWTAALPAVLTHAIATT
                     GVGLIDGIAGLFPNVPDYPVIDVAALHALLEASFPNFPLLPPLDFADLEAARFSSVLK
                     TVFPNFPDVELPDLEFPELDLAALIALLHSAIPNSHFPDSNVADAPDRHPGALIAQVH
                     NNNADFPDLDTSAQLSIPLKTDVSVSSEGDEPELTEDLNVVQESPNQAFAKGPKIEPP
                     SGPKHVLPDEPDEAALVRNASSTPPKHALNDTSKHDLHGVPEPGLKGSNTQSSETRAS
                     ESFHSPDSSPQGSAATSQSGSDKTSDRQTNGTSDRGSNRSDSSE"
     gene            complement(4918..5247)
                     /locus_tag="VFC01_27110"
     CDS             complement(4918..5247)
                     /locus_tag="VFC01_27110"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN83319.1"
                     /translation="MGGVDTHKHTHYAAVIDQHGRLLGHQQFPATDAGYRTLLSWMRT
                     HGPLEAVGVESNGSFGATLTRAPNRVADVLRHHTEPMTGIEPAYSAWESVSRCKCLFG
                     TRCCRPV"
     gene            5631..6719
                     /locus_tag="VFC01_27115"
     CDS             5631..6719
                     /locus_tag="VFC01_27115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN83320.1"
                     /translation="MAMAETGYRTAGIAGADSYWLPDHLNSLLPQAVMATKYSGAARL
                     VPDLDAFLEPWTALGYLAGRHRFNRTRLGTSVTDTGRRNPAVTAQAAATLHLMTRGRT
                     ILGIGTGEREGNEPYGVDWSKPVARFEEALATIRALWNSNGQLVNRDSEWFPLRNASF
                     TLPPYKGTWPEIWIASHGPRMLRATGRYADAWFPGALLTPEQYADGLQQVRTAADNAG
                     RNPNSIVAANFQFVVTGRSSREVEEALDSVLLRVFALNIPAWAWAEHGAQHPLGANFG
                     GFQDILPQTFDEQTALAHADAAPPSLIKQFVLTGTPDEVIDRLAKWRDHGVNYPVLCN
                     ISVLQRKLSRGVAAMLPFARILRGIRKM"
     gene            6760..7122
                     /locus_tag="VFC01_27120"
     CDS             6760..7122
                     /locus_tag="VFC01_27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HZN83321.1"
                     /translation="MESARVDRWLWAVRLTKTRPDAAAACRGGHVRVNDRAAKPATMV
                     TLGDEVRARIGDRTRIVEVVRVIQKRVGAADAVTCYLDRSPPPPPTPYVAVAVRDRGA
                     GRPTKRDRRMLDKWRASG"
     gene            7126..7896
                     /locus_tag="VFC01_27125"
     CDS             7126..7896
                     /locus_tag="VFC01_27125"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN83322.1"
                     /translation="MARLLENKVVIVAGLGGIGNGLVRRYADEGALLVIGDLDAELAL
                     RVAADLDPSRERVRGAWLDGADEDSVVSIVKLAVDTFGRLDGVHVNFTNAADAYLPGG
                     VVELSLEAFDEVMRVNTRGFVICAKHAIPAMVDSGGGSIVFTASIDAYNGASTRVSYA
                     MSKAAELALMRHIARRYGPKGIRANAIAPGLIWHYKFDEQPMPEGIVEQTRARQMIKS
                     RFGNPDDVAALGALLLSDDGSFITAQTISVDGGVTFRP"
     gene            7948..8658
                     /locus_tag="VFC01_27130"
     CDS             7948..8658
                     /locus_tag="VFC01_27130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83323.1"
                     /translation="MTEDAISIGGVDLTDPDTFAKGMPYEAFRELRQRAPVAWHPFKD
                     GPGFLALTGYDEVLAVSRDSTTWSSQETGVFYDIPLPEDAYQLTLMMLTMDPPRHTKL
                     RSLVSKGFTPRQVARLNDHIADMSRQIVDDVMEQGECDFVEDIAGALPSYVIAELLGI
                     PLEDGRRLYHLTEIMNTGTVGDPVPKTRRCRCSSTATSLRRANARIPATTSRPRCCSP
                     KWTVSVSPTSSSTCSFCY"
     gene            8604..9164
                     /locus_tag="VFC01_27135"
     CDS             8604..9164
                     /locus_tag="VFC01_27135"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN83324.1"
                     /translation="MDGERLTDFEFNMFFLLLINAGGDTTRNAAGILALLEHPAERAR
                     LAADPSLLPTAIEEMLRYTSPVTVFTRTATKDTELRGITVRKDGRVAMFYPSANRDEA
                     HFADPDTFDVGRNPNHHLAFGGGGTHFCLGANLARVEAAAIVPEVLSRMTNLELAAPV
                     ERVRSTLMNGIRSMPVRFTAAAVVQR"
     gene            complement(9155..9841)
                     /locus_tag="VFC01_27140"
     CDS             complement(9155..9841)
                     /locus_tag="VFC01_27140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83325.1"
                     /translation="MGRHSLAKKRRKPPVALAAVIGPAAVFFAVGADVHPFAAEPAPK
                     PVVGNVAPAPEAAAPCCMELVAAAPSGPSPALIVGEATTSPAHAELVSASRYRVVDRA
                     LPAGVAPEKGLQVRTILTARSISAVFPEIHEIGGVRPDALRWHPNGLALDVIIPNPSS
                     DAGIALGNQIVAYVMKNATRFGIQDAIWRGVYYTPGGGAQSGGYGHYDHVHVTTTGGG
                     YPTGGEIYYR"
     gene            10070..10768
                     /locus_tag="VFC01_27145"
     CDS             10070..10768
                     /locus_tag="VFC01_27145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN83326.1"
                     /translation="MTIELRDVVREYTVGGQTVRALDEISLRLVGGQFVSVVGPSGAG
                     KSTLLHLLGALDSPDSGSITFDGDEIGRLTDEQQSEFRHHRVGFIFQFFNLLPTLSAW
                     ENVAVPKLLDGVRLGKVKPDAVRLLDKVGLGNRTEHRPSELSGGQMQRVAVARALMMD
                     PPLILADEPTGNLDTATGASILALLAEVAHEEGRGRLVVMVTHNSDAAAATDRVITLQ
                     DGRLGSDEMSVVQG"
     gene            10765..>13145
                     /locus_tag="VFC01_27150"
     CDS             10765..>13145
                     /locus_tag="VFC01_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN83327.1"
                     /translation="MRPGATVAAAASRLRVFFMRELAVHRRRTIASIAVMAVSAMYLV
                     AVLGIFGSITGSVNRLSDGVAGIAALEVSGITDAGFPDTLAAEVAAVPGVATAAPMIR
                     MSAPTPSGPVLLFGADKSSAELGGALKDAVARPVEQLSQTPDGVQVGPAVGHGKGDTF
                     QLGSGNVTVTDVLVGGQLTDLNDGHYVLAPLALAQKVTGRQGQLDSILITTKPDADLA
                     TVRASITNAVAGRAIVAPPSMRAARAGDGVKMMNYMALMGAVVALVVGAFLIYTTMTM
                     AITQRRPVISMLRAIGGRRTTIVRDMLAEAAILGLIGGAIGSAIGILLGRIAIGRLPP
                     AITQGLEARVDYWLPGYAIPVALATTVLTSVAASAMAARQVYKVSPIEALAPVGVSAA
                     DRVPRWLRIAIGVGAIGVCGVSIAIVVGRTGTIAVGAMSALFCAEIALGFALAVPIVK
                     ATAATARVFGSVGALAAATIERSPRRVWATVMTVLIAVVTTVVITGTNADMIRSARGI
                     FSPVADVDIWVSADSPDRYPTNALPQGLSGKVAAVPGVDRVTEGAYAFADVGGTRVML
                     DGFSAGTADALYRALDERTRGDVLAGRGVVLTQNLGNSLDVKVGDQLQLQTPHGPQQA
                     AVLAVVPYFSTVIGTVGMDLDQLRAWFDRPAATTLQIATAPGVDPNQLIQDVRNVVPA
                     PNYVYEGSTALAGMEAPLRQSMFIANAVWIIVVLVAAVALLNTLTLSVLERRREIGVL
                     RAMGSSRRFTLGMVLTEAVGIGVVGGTLGLLFGLTDQWLYSLVSGDIMNFNVD"
BASE COUNT         2398 a         4277 c         4150 g         2320 t
ORIGIN      
        1 acggtggtcg gtgtatccag atacaggtcg gtcgtgttct cctctatctc gggggctacc
       61 gccggcatgg ccttcggtag cccgctcacc aattgcccac ccctgccggt gccggtcaac
      121 gtcgcccaac cttggttatc ggaaagcgtc gcgcctatgc cgcacgacac ctgacggctg
      181 cccgccagcc agcccgctgg tgacacgggg gtgtaatgca ctgtcagccc ggtcgccatc
      241 aacgagcttg gcgcaagata ttcgtcggcc attcgcgcac acgcctcctc gaggaaggca
      301 tcctgctcgc cctgggctgg caggcgtccg ccgaagcttt catctaggtt ggccgtgccc
      361 gtgacctcca ccgcgtgagg tgccgcgcag tccacggggg tgtcggtcgg ttggttgttc
      421 gccgggtcga tgcccaagca cgtacccgtc ggccagactt tcgactgatc tagttgcgcc
      481 acttggcctt tgaaaggaac cggctggcca tcggggccga gcaattgcag gccacacagg
      541 attcgtcgcc cctcgggatg cgtctctgcc accttgtctg gccagagcgc agtcaacgtg
      601 aacttgccat tgggatcgta gtggctgccc aggtaacgct gcacggccag ctggcagggc
      661 gctgcagggt ttcgcatgtc aaacgagtcc gcgacttcga acaggtgatc gtcctggcac
      721 tggacgaagg acggctggcc tggtgcgttc gccggccaat tcaggcagtc gccgctctgc
      781 gcgctgccga taacgggggc ggcctggagt cccgcttcgc ggtcgttgcg tacgtccggc
      841 ttggcatcct gaccacccag ctccaaggcg gtgatgatgc cgccgacgag aagtccgcag
      901 agcaccggtg acaaaactcg ccgcgctgat gcgccccgga cgatgtgccc ccatggctcc
      961 ttgatcggtt catcgaccgc cgattggtcg gactcatcag actcgaaccc tgcttgacga
     1021 ttcgtcaacg acaagaagac gtcgtcaagt gagggcctgc gtagctgaat atcggccaac
     1081 tcaatgtgcg cagcgctgag ccgcgacatc gcctcaatga gcgtactcac gccgtctggc
     1141 gcaggcacgg cgactcgatc cgaatcggac gtcaacgccg ccttgttctg ctccggcatg
     1201 agcgagccga gcgcgtctgc tgttgcgtgc agatcattgc ggtcccgcgg agttatctcg
     1261 cagtaaccac ctccggtgcg ctctttaagc tcgtccgcgg tcccctcggc gacgatcaca
     1321 ccgtgatcga tgacaatgat ccgatcactg agcaggtccg cctcctcgag atactgcgtc
     1381 gtcaacagcg tcgcgatccc gaggttcttg aagtcggcca ctagatccca gatggtttgc
     1441 ctgcttctcg gatcaagccc cgtcgtgggc tcgtcgagaa acaccacctc aggagggacc
     1501 actaaaccgc aggcaatatc tatccgcctg cgcattccac ccgaaaacgt gcccacgcgt
     1561 cgatgggcgg catgcgccag atcgaacttg tggagaagtt cctctgcccg agaccgggat
     1621 tccgagctac tgaggtcgcg caaacgcccg aacagaatca ggttctctcg ggccgtgagc
     1681 atgtcatcga gcgccacatg ctgtccggtg agcatgatcg attgacgtac gccggccgcc
     1741 tcggacacca cgtcgaatcc ggcaacggtc gcgtggcccc ggtctggccg cgcgagcgtc
     1801 gacaggatat tcaccgtcgt cgtctttccg gccccgttcg gacccagtaa ggcgaccact
     1861 tcaccacggt cgacgtcgaa actcacgtcg cgtagggcat gtacggcacc aaacgactta
     1921 ccgagtccac tgacgaccac ggccttctct gcggtgggca ccccgggctc ccggaccagt
     1981 ggttccttcg tcgctacgga gctcaattgc accgtggcgc attccgccga gagatccacg
     2041 gagccgtttt gctcgggcgc gccgttcaat tcggtgccag cacgacgtgc ggccattgct
     2101 tcctttattg gttaggtgag tgggcagagg tcgtcaacta tagggataga cccttacaca
     2161 ggtggttcgg tcctgccttc ttgtggacta acaagttccc tagcagatgc tggttcgcaa
     2221 ttgtcgcgca gcagaaagaa gcgatgcccg aactcccaca aggtacgccc atacccgcct
     2281 cacgggttgg acgttgctcg caaaatccgg cagacgaagc cagccgcccg ccaaggcacg
     2341 gcagattcct gaccaaggtg cggactggcg gcgccaccac caaggcagct agtcggcaaa
     2401 cgcttcgccg accgctccac gcatagttct cttttgctgg agaacggctc gaccgctatc
     2461 cagcgagagc ctgggcacga acggaaaact tcgtaagtgc cttttacatc gacggcgtag
     2521 taaataccgg ccagttcggt cgctaggcct atcgatcccc gacactacca gggcgatgag
     2581 tacaccacct cgactccgag tggcaaatcc tgactagcgg aactagctct ttgcggtgct
     2641 gatgagatga gctaatatcc gctggccaaa cacgggtaca tctgctctcg acatttgctt
     2701 caagatcagc cactgccgac tttgaatgga atagcaaagg ccaaagcgtt ttggcagcaa
     2761 gtgcaccggg tacatcgaag ccagcagaat gagttggccg gaccgacccc attcgcaagt
     2821 cgcttgatga ctggcaaagg cagaccaatg tcccacccga ggaatcactc ccgaaatcgt
     2881 gcaacgtctc agccgcccaa gcgaagcggc cgtcaccttc ggaagcggca gaagtcatcc
     2941 agcagcgcag cgggtgccgc gttgactggc gctccgtttt cgtcggggcc gcccgacttc
     3001 gaccggccgt tgcggcaacg aggccatcgc ccccaacatc ggcgaccgtc ggtgtgccgc
     3061 ccctacgtga caaccgggat cgcgatagta ggagccagcg ccgttgttgg cagctcgatc
     3121 acgccgcctg cggcgcgcgt tgagacgacc cgtctggaac tcaccgttgc cgaaagcagt
     3181 gcgccgatgc cgaatccgct gctggcggca gtcgtcgaaa cgagcacgag gagcagctgc
     3241 gaagctgtcg acgctcgagt cgacggctgg tgtgccggcg ccttcccgtc actgttcacg
     3301 cccgtcgcgg gagtgctcgg gaccggcctc gacatatggg acaggacggt cggtctgggg
     3361 gcatccgtgg ttgccggcac tgcaatcacg gcgggcttga taggggtagg gctcgatacc
     3421 gccctggccg aagctctcct aacaccgctt gaggtgctcg tcgagggcgg tgtgctgccc
     3481 gcacagctgg ctacatccat cgcggcgatg gtcggaggtt tcactaccgg tgtcaacatc
     3541 ggaattgcgg gcgtcaccac cggtattgct tggaaagccg ccttggacgc cgccctggcc
     3601 aacaccatag ccgccgctgg agcggacatc atcaatgcgc tgatcgccgg cttcccgaat
     3661 atggatttcc ccgcggcaat cgctgcagtg atcgggggct tggcggcgat gggtgagact
     3721 gccctcactt ggttggacgc cacggtcacc ctcggcgcat cagtggttgc cggcatcgcc
     3781 atgacggcgg ccacttctgt agtagagctc aacactgccc tcactggggc attcctggaa
     3841 tgcctcgagg cgctcgccga gagtggcgta ctacccgctt cggcggccac ggcccttgca
     3901 gggctggtcg gatcagtctc ctctgctgcc aatgccggca tcctcggcct gggcagtgcg
     3961 gtcgcgtgga cggccgcctt gcccgctgtg ctcacccacg ccattgccac cacgggcgtc
     4021 ggcctgatcg atggcattgc cggcctgttc cccaacgttc cggactatcc cgttatcgac
     4081 gtggcggcgc tgcacgcact gctcgaggcg agcttcccca acttcccgct cctgccgccg
     4141 ctcgatttcg ccgatctcga ggcggcgaga ttcagttctg tgcttaaaac cgttttcccg
     4201 aacttcccgg acgtcgaact tccggatctg gaattcccgg aactcgacct ggccgctttg
     4261 atcgccctac tgcacagcgc gattcccaat tcccacttcc ccgacagcaa cgtcgccgac
     4321 gcccctgatc gacacccggg ggcgctcatc gcgcaggtgc acaacaacaa tgccgacttc
     4381 cccgatctcg acacctccgc ccaactcagc atcccgctga agaccgatgt ctccgtctct
     4441 tcagagggcg acgagcccga gttgacggaa gacctgaatg tcgtccagga gtccccgaac
     4501 caggcgtttg ccaagggacc aaaaattgag cccccctcag gtcccaagca cgtcctgccg
     4561 gacgaacccg acgaggcagc gttagtaagg aacgcctcgt caacaccacc caagcatgcg
     4621 ttgaacgaca cgtcgaagca tgacttgcac ggcgtgccgg agccgggtct gaagggatcg
     4681 aatacccaat cgtcggaaac acgcgcgtcc gagtctttcc atagtcccga ctcttcaccg
     4741 caaggaagtg ctgcgacatc ccagagtgga tcggataaga cctccgacag gcagacgaac
     4801 ggcacttcgg acaggggctc gaacaggtcc gacagcagcg aataactttg cggctgagga
     4861 tttgacgcat agcggcaccc ggcagttggg cgaaggcgct cgaggcaccg ctgaagatta
     4921 gacgggccta caacaacggg taccgaacaa gcatttacac cgagaaacgc tttcccaagc
     4981 tgaatacgcg ggttcgattc ccgtcatcgg ctccgtgtga tgtctcaaga catcggcgac
     5041 gcggttgggc gcgcgagtca gcgttgcgcc gaacgatccg ttgctttcca cgccgacggc
     5101 ctcgagcggt ccatgtgtgc gcatccacga cagcagtgtg cggtagccgg cgtcggtcgc
     5161 cgggaactgc tgatgaccaa gcaaccgccc gtgttgatcg atcaccgcgg catagtgagt
     5221 gtgtttatgg gtgtcgacgc caccgatgcg aggcttcgca gagcggagtc gtgagcaacg
     5281 tgattgctat ggctacggag accgcggcca cggttgtcca gaacacgatg aaccacggag
     5341 tacgcacccg tttgctgaag tcgcgaagat tcatggcccg gtcaatgcta gtcacccgag
     5401 catcgtgatc cgcgctgcca cgtcgggatg cgaagcatac cggcgcagtg cccgcagagc
     5461 gggccggtac gtaccgcaca tgcgggcgaa cagcgggaca aaaggaaagc tgatcgaaac
     5521 cgcgctgatc cgaccgcagc gagccatagt cttttggcat ccgccggggg gaggcatttg
     5581 cgatgaagtt cggtgtctgc gagcagccgt tgcacagccg cgtcgggcca atggccatgg
     5641 ccgaaacggg ctatcggacc gccgggatag ctggtgccga ctcgtactgg ctacccgacc
     5701 acctcaactc cttgttgccg caggcggtga tggcaacaaa gtacagcgga gctgctcggc
     5761 tcgtgcccga cctcgacgca ttcctagagc catggacagc gctgggctac ctcgcggggc
     5821 ggcaccgatt caaccggacg cgtctgggca cctccgtgac cgataccgga cggcggaatc
     5881 cggccgtcac ggcgcaggcc gcggcgacgc tgcacctgat gacaagaggc cggacgattc
     5941 tcgggatcgg aaccggcgaa cgcgaaggca acgagccgta cggggtggac tggtcgaagc
     6001 cggtggcacg gttcgaagaa gccctcgcaa ccatacgggc gctatggaac tcgaatgggc
     6061 agctggtgaa ccgcgattcg gaatggttcc cgctgcgcaa cgcgtccttc acgttgccgc
     6121 cttacaaggg cacctggccc gagatctgga tcgcgtcgca cggaccgcgg atgctgcgcg
     6181 cgacgggtcg ctacgccgat gcgtggttcc cgggagcgct cttgactcct gaacagtacg
     6241 cagacgggtt gcagcaggtc cgaacggccg ccgacaatgc cggccggaat ccgaactcca
     6301 tcgttgcggc caacttccag ttcgtcgtga ccgggcgaag ctctcgggaa gtcgaagaag
     6361 cgttggattc ggtgctgcta cgggttttcg ccctcaacat tccggcttgg gcgtgggccg
     6421 agcacggcgc tcagcacccc ctcggcgcga actttggcgg ctttcaggac attctgccgc
     6481 aaactttcga tgagcagact gcgctcgctc acgccgacgc agcgccacca tccctcatca
     6541 agcaattcgt gctgactgga acacccgacg aggtcatcga ccggctagct aagtggcgcg
     6601 accacggcgt gaactacccc gtgctgtgca acatcagtgt cttgcaaaga aagctgagcc
     6661 ggggcgtcgc ggccatgctg ccgttcgcga ggatcctgcg gggaatccgc aaaatgtaag
     6721 tacccggacc ctcggtgtcg gcagtccatg atggatgtca tggagtcggc ccgcgtggac
     6781 aggtggttgt gggcggtccg gctgacgaag acccggccag acgccgctgc ggcgtgccga
     6841 ggtggacacg tgcgggtcaa cgatcgagcg gcgaagccgg cgaccatggt gactctcggt
     6901 gacgaggtgc gggcccggat cggtgacagg acgcggatcg tcgaggtggt acgggtgatt
     6961 cagaagcggg tcggcgccgc cgatgccgtg acctgctacc tcgaccgatc accacctccg
     7021 ccgccgacgc cgtatgtcgc ggttgccgtc cgagatcgcg gcgccggacg gccgaccaaa
     7081 cgggaccgcc ggatgctcga caagtggcgg gcgagcggct gagcgatggc gagactgctc
     7141 gaaaacaagg tcgtcatcgt tgcggggttg ggcggcatcg gcaatggcct ggtgcgtcgc
     7201 tatgccgatg agggcgcact gctcgtgatc ggcgatctcg acgccgagtt ggcgttgcgc
     7261 gtggccgccg acctcgaccc gtcgcgtgag cgtgtgcgcg gtgcatggct cgacggcgcc
     7321 gacgaggatt ccgtcgtctc gatcgtcaag ctcgccgtcg acacgttcgg ccgcctcgac
     7381 ggtgtgcatg tgaacttcac caacgcggcc gacgcgtacc taccgggcgg tgtcgtcgag
     7441 ctgtcgctcg aagcgttcga cgaagtcatg cgcgtcaaca cccgcggttt cgtgatctgc
     7501 gccaagcacg cgattcccgc gatggtcgac agcggcggcg gttcgatcgt gttcaccgcg
     7561 tcgatcgacg cctacaacgg cgcgagcacc cgcgtgtcgt acgcaatgag taaagcggcc
     7621 gagctggcgc tgatgcgtca tatcgcgcgc aggtacggcc cgaagggcat tcgcgccaat
     7681 gccattgcgc caggactgat ctggcactac aagttcgacg aacaaccgat gccggaaggg
     7741 atcgtcgagc agacacgcgc acggcagatg atcaagtccc gcttcgggaa cccggacgat
     7801 gtcgccgcat tgggcgcgct gctgctctcc gacgatggca gcttcatcac ggcgcagacg
     7861 atcagcgtcg acggcggagt gacctttcgg ccctgactgc cggtcgatct tccaacgaaa
     7921 cgcgcccgac gggtctaacg tgcgagcgtg actgaagacg cgatcagcat cggcggcgtc
     7981 gacctcaccg accccgacac cttcgccaaa ggcatgccct acgaggcatt tcgtgagctt
     8041 aggcagcgcg cgcccgtcgc ctggcacccg ttcaaggacg gaccgggatt cctcgcactc
     8101 accggttacg acgaagtcct tgccgtctcg cgcgacagca ccacgtggtc atcacaggag
     8161 accggcgtct tctacgacat tccgctgccc gaggacgcct accagctcac gctgatgatg
     8221 ctcacgatgg acccaccgcg gcacacaaaa ctgcgctcgc tggtgagcaa gggcttcaca
     8281 ccgcgtcagg tcgcgcggct gaacgatcac atcgcggaca tgtcacgcca gatcgtcgac
     8341 gacgtaatgg aacaaggcga atgcgacttc gtcgaagaca tcgccggcgc gctgccgtcc
     8401 tacgtgatcg cagaactgct cggcatcccc ctagaagacg gtcgccggct ctaccacctc
     8461 accgagatca tgaacaccgg cacagtcggg gaccccgtgc caaagacgcg cagatgcaga
     8521 tgttcgtcta cggcaacgag cttgcggcgc gcaaacgcac ggatcccggc gacgacatcg
     8581 cgacctcgct gctgctcgcc gaagtggacg gtgagcgtct caccgacttc gagttcaaca
     8641 tgttctttct gctattgatc aacgccggag gcgatacgac gcgcaacgcg gcgggcatcc
     8701 tcgcgctgct ggagcacccc gccgagcggg ccaggctggc ggcggacccg tcgctgctgc
     8761 cgactgcgat cgaggagatg ctgcgctaca cgagcccggt caccgtcttc acgcgcacgg
     8821 ccaccaagga cacggaactg cgaggaataa ccgtgcgcaa ggacggccgg gtcgcgatgt
     8881 tttacccgtc ggccaaccgc gacgaagccc acttcgctga tcccgacacg ttcgacgtcg
     8941 gccgcaatcc gaatcaccat ctggcattcg gcggcggcgg gacccatttc tgcctcggcg
     9001 ccaacctcgc ccgggtggag gccgccgcga tcgttcccga agtgctctcg cgcatgacga
     9061 acctggaact cgccgcgccg gtcgagcgag tgcgctcgac actcatgaat ggcatccggt
     9121 cgatgcccgt tcgcttcacc gcggcagcgg tcgttcagcg gtagtagatc tcgccgccgg
     9181 tcggataccc gccaccggtg gtcgtgacgt ggacatggtc gtaatggcca tacccaccgc
     9241 tttgcgctcc gccgccgggc gtgtagtaca cgccgcgcca gatcgcgtcc tgaatcccga
     9301 atcgagtcgc gttcttcatc acgtaagcca cgatctggtt gccaagagcg atgcccgcat
     9361 cgctgctggg gttgggaatt atcacgtcga gcgccagacc gttcggatgc cacctcagcg
     9421 catccggtcg aacgccgccg atctcgtgaa tctcggggaa taccgcgctg atactgcgcg
     9481 cagtcaagat cgttctgact tgcagaccct tctccggcgc gaccccggcg ggcagcgcac
     9541 ggtcgaccac ccgatatcgc gatgccgaaa cgagctcggc gtgagcggga ctcgtagttg
     9601 cctcacccac gatcagcgcg ggcgagggtc cactcggtgc ggccgccaca agctccatac
     9661 agcacggcgc agcggcttct ggggccggcg caacattgcc gactaccggt ttgggggcgg
     9721 gctcggcggc gaacggatgg acatcggccc cgacggcgaa gaacaccgcg gcggggccga
     9781 tgacagcagc cagggcaacc ggcggcttgc gccgcttctt ggctaatgag tgccggccca
     9841 cggaaagcac aatacgaaaa aactggggca aatgtcacgg agttgtcacg gagttatcac
     9901 gaaacggcac atttccgcag gtcagcgatc ttgatgcacg acaggatcgt gatccccata
     9961 tgtggtcgag cacgccgcaa cgcgacggga gtaggctgct gatgcctcga cagcaaactt
    10021 tgcatcgtcg gatgggggcg cggtgagcga tacctccgaa acacgcgaac tgaccatcga
    10081 actgcgcgat gtagtgcgcg agtacacggt cggcggccaa acggtgcgtg cgctcgacga
    10141 gatcagcctg cgtctggtcg gtgggcagtt cgtatcggtc gtcgggccgt cgggcgcggg
    10201 aaagagcacg cttttgcatc tgctcggcgc attggactcc cccgactccg gatcgatcac
    10261 attcgacggc gacgagatcg gccgcctcac cgacgagcag cagtcggagt ttcgtcatca
    10321 tcgcgtcggc ttcatcttcc agttcttcaa cctgttgccg acactctcgg catgggagaa
    10381 cgtcgcggtg ccgaagctcc tcgacggcgt ccggctcggc aaggtcaaac ccgatgcggt
    10441 tcgactcctc gacaaggtcg gactcggtaa ccggaccgag caccggccct cagaactctc
    10501 gggcggccag atgcagcggg tcgcggtcgc gcgggcactg atgatggacc caccactgat
    10561 tcttgccgac gaaccgaccg gaaacctcga caccgccacg ggcgcttcga ttttggcgct
    10621 ccttgccgaa gtggcacacg aggaaggccg cggtcggctg gtggtgatgg tgacccacaa
    10681 ctcggatgcc gcggcggcga ccgaccgggt gatcacactg caagacggac ggctcggttc
    10741 cgacgagatg tcggtggtac aggggtgaga cccggtgcga cggtcgctgc cgcggcgagc
    10801 cggctgcggg tgttcttcat gcgggagctc gctgtccatc gccggcgcac gatcgcctcg
    10861 atcgccgtaa tggcagtgtc cgcaatgtat ctggtggcgg tgctcggcat cttcgggtca
    10921 atcacggggt cggtcaaccg gctgtccgac ggggttgcgg ggatcgctgc gctcgaggtg
    10981 tcgggcatca ccgacgccgg gttccccgac accctcgcag ccgaagtcgc cgcggttccc
    11041 ggcgtcgcga ccgcggcgcc gatgatacga atgtcagcgc cgacgccgtc cgggccggtg
    11101 ctgctgttcg gcgcggacaa gagcagtgcc gagttgggtg gcgcgttgaa agacgccgtc
    11161 gcgcgaccgg tggaacagct gtctcaaacg ccggacggtg tccaggtagg gcccgccgtc
    11221 ggtcacggca agggtgacac gttccagctc ggctcgggga acgtcaccgt taccgatgtc
    11281 ctggtgggtg ggcaactcac ggacctcaac gacggacact acgtcctagc tccacttgcc
    11341 ttggcgcaga aggtcaccgg ccgacaaggc cagctcgact cgatcctgat caccacgaaa
    11401 ccggacgccg atctcgcgac agttcgtgcc tcgatcacca atgccgtggc cggccgtgcg
    11461 atcgtcgcgc cgccgagtat gcgggcggct cgggccggcg acggcgtcaa gatgatgaac
    11521 tacatggccc tgatgggcgc ggtggtcgcg ttggtggtgg gcgcgttcct gatctacacg
    11581 acgatgacca tggcgatcac gcagcggcgg ccggtcatct cgatgctgcg cgcgatcggc
    11641 ggccgccgca ccacgatcgt ccgcgacatg ctcgccgagg cagccattct cggactgatc
    11701 ggcggcgcaa tcgggtcggc catcggaata ctcttgggcc gcatcgcgat tggccgattg
    11761 cctccggcaa ttacgcaagg gctcgaagct cgcgtcgact attggctgcc cggctatgca
    11821 atacctgtcg ccctcgccac gacggtgctc accagcgtgg cggcgtcagc gatggcggcc
    11881 cggcaggtgt acaaggtgtc gccgatcgag gcgctggcac ccgtcggggt gtccgcggcg
    11941 gaccgcgtac cccgatggct gcggatcgcg atcggcgttg gcgccattgg ggtttgcggc
    12001 gtgtcgatcg cgatcgtcgt cggccgcacc ggcaccatcg ccgttggcgc gatgtcagct
    12061 ttgttctgcg ccgagatcgc gctcggcttc gcgctcgccg tgcccatcgt caaagccacc
    12121 gcggcaaccg cccgagtgtt cggatcggtc ggggcgctcg cggcggcgac gatcgagcgt
    12181 tcgccacggc gagtctgggc caccgtgatg accgtgctca ttgccgttgt caccaccgtc
    12241 gtgatcaccg gtacgaacgc cgacatgatc cgctcggcgc gcggtatctt ctccccggtc
    12301 gcagacgtgg acatctgggt gagcgccgat tcccccgaca gatatcccac caatgctctg
    12361 ccgcaaggcc tttccggcaa ggtggctgcg gtgcccggcg tcgaccgcgt cacagagggc
    12421 gcatacgcgt tcgctgacgt cggcggcaca cgcgtgatgc tcgacgggtt ctcggccggc
    12481 accgccgacg cgctttaccg tgcgctcgac gaacgaaccc gcggcgacgt gctcgccggt
    12541 cgcggcgtgg tgctgaccca gaacctgggc aactcgctgg acgtcaaagt cggtgaccaa
    12601 ctgcaattgc agacgccaca tggtccgcag caggcggccg tgctggccgt ggtgccgtac
    12661 ttctcgaccg tcatcgggac cgtcggaatg gacctcgatc aactgcgtgc gtggttcgac
    12721 cgtccggccg cgacaacgct ccaaatcgcc actgccccag gcgttgatcc gaatcaacta
    12781 atccaggacg tccgcaacgt agtgcccgcc ccgaactacg tatacgaagg cagcacggcg
    12841 ctggcaggca tggaggcgcc gctgcgtcag agcatgttca tcgccaacgc cgtgtggatc
    12901 attgtcgtgt tggtcgccgc cgtcgcactt ctcaacacgc tcacgctttc cgtgctcgaa
    12961 cggcgccgcg aaatcggcgt gctgcgggcg atgggctcca gtcgccgatt caccctggga
    13021 atggtgctga ccgaagcggt gggcatcggt gtcgtcggcg gtaccctcgg cctgctgttc
    13081 ggcctgaccg accagtggct ctacagcctt gtcagcggcg acatcatgaa cttcaatgtc
    13141 gactt
//
LOCUS       DASRJA010000611        24524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_355817,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000611 DASRJA010000000
VERSION     DASRJA010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 24524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24524
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355817"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VFC01_27155"
     CDS             <1..870
                     /locus_tag="VFC01_27155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN83328.1"
                     /translation="LLNAEEEVELAKRIEAGLYATQLMLELTEKGEKLPAAQRRDMSW
                     ICRDGDRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGY
                     KFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPTPEEL
                     AKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFTLLQ
                     DQLQSVLETLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIESKTMSKLR
                     HPSRSQVLRDYLD"
     gene            948..1445
                     /locus_tag="VFC01_27160"
     CDS             948..1445
                     /locus_tag="VFC01_27160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83329.1"
                     /translation="MRVSDNALRKLIKGTFLATLLLAAMEGGTPLHEATWIFVGAVLT
                     TVVDAYATHMSGRHEGGMPAYFGSLYRGVVGDLPRVIGSLPTVLLLVFAWMFQWHHDL
                     RNPDGSVSVGYETVALNVNVVLLFIFSILAAYRSGASWRGTVIFALMNAALGWIIVTV
                     ELALE"
     gene            1542..2642
                     /locus_tag="VFC01_27165"
     CDS             1542..2642
                     /locus_tag="VFC01_27165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZN83330.1"
                     /translation="MSTAEKPPRIPPLALVRAINAVRAGAQRVSRALAPGGLNVMELL
                     TGAWTAQTIYVAVKLGIPDELADGPLPADEVARRVVADPDAVYRLMRALASRGVLRHR
                     TDDTFKLTSIGKALRTGTPGSMRDMALFLGHPLRWEDWGNLLYSVETGKPSVEKLRGM
                     PFFEYVETDTDLAEAFNNAMTAGSEFSIYAVLAAYDFSGYRKIIDIGGGHGRLLSMIL
                     ARAPHARGVLFDLPNVVDGATEELTKAGVAERCDVVGGSFFDAVPENGDAYLMKAILH
                     DWSDEDALKILSTIRAAIAPGGKLVLLESVLPERSSSDIGLLIDLEMLVAVGGRERTH
                     AEWANLLGRAGFRLSRVVRTATPVSIVEAVPA"
     gene            complement(2639..3421)
                     /locus_tag="VFC01_27170"
     CDS             complement(2639..3421)
                     /locus_tag="VFC01_27170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83331.1"
                     /translation="MGILLGFAPWIVYWVLVGNVPFTTAVLVALAVAVLSFAIGRVRG
                     APGRTLEIGAVATFLILTVLTFTLSQSFMERWMQPLSNAGIFLVAFVGVLAGRPFVRE
                     FAEVDQPKEVVESELFQRITTLLTWIWVAAFAGMTVSSAIPPIVYGEATILDTKTPLS
                     FVCYWVIPFSLLGLAALASRVLPEKMTPPPEDIVRKTTFVAFAEAEIDQLIYLATEHA
                     NREVGAGKEPYDIRIGSKGVPLVGDETRESWPSTYKVRDKKR"
     gene            3502..3870
                     /locus_tag="VFC01_27175"
     CDS             3502..3870
                     /locus_tag="VFC01_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZN83332.1"
                     /translation="MAQRRTVSSGSEYESAVGYSRAVRTGPHIVVAGTTGQGHGIAAQ
                     TREALRRIDGALHQLGASLSDVVRTRIYVTEISLWQEVGAVHAEVFGEIRPVTTMVEV
                     SALISPDLLVEIEVDAYLDA"
     gene            complement(3867..4220)
                     /locus_tag="VFC01_27180"
     CDS             complement(3867..4220)
                     /locus_tag="VFC01_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HZN83333.1"
                     /translation="MYAAPSVLLHLCSNDEWRSAQARGEHRPDSLDECGFVHLSTTEQ
                     VHLPANRLYAGRTDLVLLHIDPARLTSPVRWEPGVATDPDAMVFPHLYGALPLEAVIN
                     VTPYRPDEGGRFAPL"
     gene            4352..4543
                     /locus_tag="VFC01_27185"
     CDS             4352..4543
                     /locus_tag="VFC01_27185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83334.1"
                     /translation="MNATLTSPELTRADRCDRCGAAARVRAKLPSGSELLFCQHHANE
                     HEAKLVELAAVLEVSPLEP"
     gene            4571..5563
                     /locus_tag="VFC01_27190"
     CDS             4571..5563
                     /locus_tag="VFC01_27190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZN83335.1"
                     /translation="MDDQSQPIPVPPSRHHIRRLVRRTLSKSWDDSIFSESAQAAFWC
                     ALSLPPLLLGMLGSLAYIAPLFGPDTLPTIQEQLISTAGRFFSPNVVNEIIAPTVRDI
                     VKGARGEVVSIGFVISLWAGSSAISAFVDSITEAHGQTPLRHPVRQRFYALGLYVVML
                     VGAIVTAPFIALGPRKIAEYLPDSWDSVLAYGYYPVLFVGLVVAMNVLYRVSLPKPLP
                     SHRLLIGSVLATAVFLIATYGLRVYLTWITRTGYTYGALATPIAFLLFAFFLGFAIML
                     GAELNAAIQEEWPAAATHAKRFRWWLEEKAESLNGNGPVITQPAATAADGDATS"
     gene            complement(5553..5789)
                     /locus_tag="VFC01_27195"
     CDS             complement(5553..5789)
                     /locus_tag="VFC01_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HZN83336.1"
                     /translation="MRTQTIERTDTDERVDDGTDSDTPKYFHYVKKDKIAESAVMGTH
                     VVALCGEVFPVTRAAKPGSPVCPECKRIYEKLKK"
     gene            5856..6257
                     /locus_tag="VFC01_27200"
     CDS             5856..6257
                     /locus_tag="VFC01_27200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HZN83337.1"
                     /translation="MKHGQELSFDDEGRPVLITRAAAPYEEQHRERVRKYLTLMFWRI
                     PALILAAVAYGIWENGLISLAIILVSVPLPWMAVLIANDRPPRRAEEPRRYDSRRRIP
                     LFPTAERPALERGPHSAPQPDGGASPGDVPS"
     gene            6405..7364
                     /gene="sigB"
                     /locus_tag="VFC01_27205"
     CDS             6405..7364
                     /gene="sigB"
                     /locus_tag="VFC01_27205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor
                     SigB"
                     /protein_id="HZN83338.1"
                     /translation="MANATTTRVDHDLDAQSPAADLVRVYLNGIGKTALLNAADEVEL
                     AKRIEAGLYAQHLLETRKRLGENRKRDLAAVVRDGEAARRHLLEANLRLVVSLAKRYT
                     GRGMPLLDLIQEGNLGLIRAMEKFDYSKGFKFSTYATWWIRQAITRGMADQSRTIRLP
                     VHLVEQVNKLARIKREMHQNLGREATDEELAAESGIPVEKINDLLEHSRDPVSLDMPV
                     GSDEEAPLGDFIEDAEAMSAENAVISELLHTDIRHVLATLDEREQQVIRLRFGLDDGQ
                     PRTLDQIGKLFGLSRERVRQIEREVMAKLRNGERADRLRSYAS"
     gene            7471..8163
                     /locus_tag="VFC01_27210"
     CDS             7471..8163
                     /locus_tag="VFC01_27210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HZN83339.1"
                     /translation="MNDLVDTTEMYLRTIYDLEEEGVVPLRARIAERLEQSGPTVSQT
                     VSRMERDGLLHVAGDRHLELTDKGRSLAISVMRKHRLAERLLVDVIGLPWEEVHAEAC
                     RWEHVMSEDVERRLVQVLNNPTTSPFGNPIPGLSELGVGDDLASDQINLVRLTELPSG
                     MPVAVVVRQLTEHVQGDAELIAKLKDAGVVPNARVTVEVNDHGGVMIVIPGHEQVELP
                     HHMAHAVKVEKV"
     gene            complement(8216..9262)
                     /locus_tag="VFC01_27215"
     CDS             complement(8216..9262)
                     /locus_tag="VFC01_27215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 domain-containing protein"
                     /protein_id="HZN83340.1"
                     /translation="MTKQLPDFHLNRPGVLIAALPAVLGFVPEKSLVLVTVDRGEMGC
                     VMRVDLSDELQHSLAHIADVAAAAKPDSAIAVVIDEDGASCRMCNDEYRELADRLTTA
                     LANRSIELLAAHVVDRVAAGGRWHCADGCGNAGTVDDPSASPLAVAAVLDGRRLYARR
                     AELQEVIEVANSARADLLAEIIGDRKPDSAERPDEAARADIEATIAAAARVADAVELD
                     DDELARLAWALTDLRVRDTLYALAVGENAGQAESLWALLSRTLPEPWRVEALVLLAFS
                     AYARGDGPLAGVSLEAALRGDPTHRMAGMLDTALQSGLRPEKIRDLAITGYRLADRLG
                     VQLPPMRSFGRRAG"
     gene            9441..10853
                     /gene="sthA"
                     /locus_tag="VFC01_27220"
     CDS             9441..10853
                     /gene="sthA"
                     /locus_tag="VFC01_27220"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HZN83341.1"
                     /translation="MGSMEYDLVVIGSGPGGQKAAIAAAKLGKSVAVVERGRMLGGVC
                     VNTGTIPSKTLREAVVYLTGMSQRELYGSSYRVKEKITPGDLLARTTHVINKEQDVVR
                     NQLMRNRVDLHTGHARFLDEHTVCVDDQTRGERTTVRGEYIVIATGTKPARPTGVEFD
                     EERVLDSDGILDLNSLPATMVVVGAGVIGIEYASMFAALGTKVTVVEKRENMLDFCDP
                     EIVEALKFHLRDLAVTFRFGEEVTAVDVGSAGTVTTLASGKQIPAETVMYSAGRQGQT
                     EHLDLTNAGLETDGRGRIVVDSQFQTKVDHIYAVGDVIGFPALAATSMDQGRLAAYHA
                     FGEPAKGMTDLQPIGIYSIPEVSYVGATEVELTKNAIPYEVGVSRYRELARGQIAGDS
                     YGMLKLLVSTDDLKLLGVHIFGTSATEMVHIGQAVMGCGGTVEYLVDAVFNYPTFSEA
                     YKVAALDVMNKLRALTQFRA"
     gene            complement(10859..11566)
                     /locus_tag="VFC01_27225"
     CDS             complement(10859..11566)
                     /locus_tag="VFC01_27225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN83342.1"
                     /translation="MEQAARVEAKAPPTAQPVPSDPRVGAVFLGAGTMHTCSGAVLDS
                     ATGDLILTAAHCVAEGVDATFVAGLKDAAAPADVWHIDAVYLDPRWVQNQDPLADFAI
                     ARVSRDSGGSVQAQAGSGLTIGPAPKPGTVVDVTGYAMGDGGGPIGCRTATAPPAQGF
                     PALDCAELVNGLSGAPWIEGSRVTGLIGGLKGGGCEDESVSYSPPFDDAIKRLFTRAE
                     ASGPADAAPTVFDDDCD"
     gene            11764..12741
                     /locus_tag="VFC01_27230"
     CDS             11764..12741
                     /locus_tag="VFC01_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HZN83343.1"
                     /translation="MAENADRPEHHYQPEQTGMYELEFPAPQLSSADGSGPVMIHALE
                     GFSDAGHAIRLAAQHLKNHLDTELVASFAIDELLDYRSRRPLMTFKTDHFTSYEEPEL
                     NLYALHDSVGTPFLLLAGMEPDLRWERFITAVRLLAERLGVRRVIGIGTIPMAVPHTR
                     PITMTAHANDKELIADHTPWVGEVVVPGSVSNLLEFRMAQHGHEVVGFTVHVPHYLAQ
                     TDYPAAAQALLAEVAKSGSLQIPLNTLTQAAAEVHDKINEQVEASTEVAQVVEALERQ
                     YDAFVAAQENRSLLARDEDLPSGDELGAEFERFLAQQTGDKPNDDDDNK"
     gene            12769..13791
                     /locus_tag="VFC01_27235"
     CDS             12769..13791
                     /locus_tag="VFC01_27235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83344.1"
                     /translation="MTARKPNLRPVRELTPTLQFRTIHGYRRAFRVAGSGPAILLIHG
                     IGDNSTTWSTVQTKLAQRFTVIAPDLLGHGKSDKPRADYSVAAYANGMRDLLSVLDID
                     NVTVIGHSLGGGVAMQFAYQFPQLVDRLILVGAGGVTKDVNVALRIASLPMGSEALAF
                     LRLPLVLPAMQVVGRVAGAVFGSTGMGRDLPDVLRILADLPEPTASSAFARTLRAVVD
                     WRGQVVTMLDRCYLTQSVPVQLIWGSRDSVIPVSHARMAHAAMPGSQLEIFERSGHFP
                     FHDDPDRFVEVVERFIGSTDPAVYDQEYLRSLLRAGISESTIAGPVDTRVAVLDAMGA
                     DERSAT"
     gene            13817..14503
                     /locus_tag="VFC01_27240"
     CDS             13817..14503
                     /locus_tag="VFC01_27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZN83345.1"
                     /translation="MAIDVTVLRVFTDQDGNFGNPLGVVDASQAEPSERQRIATQLGY
                     SETIFIDVPRAGASTAHARIYTPATELPFAGHPTVGASWWLRDRGTPVRTLQVPAGIV
                     QVAYEDDLTAVSARSDWAPEFAIYDLDSTDDLFAANPDEYPDDIQNYLWTWTDRERGA
                     IRSRMFAANLGVPEDEATGAAAVRITDYLSRDLTILQGKGSVIETQWSPEGWVRIAGR
                     VVNDGTKQVD"
     gene            complement(14518..14982)
                     /gene="nrdR"
                     /locus_tag="VFC01_27245"
     CDS             complement(14518..14982)
                     /gene="nrdR"
                     /locus_tag="VFC01_27245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZN83346.1"
                     /translation="MHCPFCRHPDSRVVDSRETDEGQAIRRRRSCPECGRRFTTVETA
                     VLAVVKRSGVTEPFSREKVIRGVRRACQGRQVDDDALNLLAQQVEDSVRATGSPEVPS
                     HEVGLAILGPLRELDEVAYLRFASVYRSFESAEDFEREIEALRAHREVPAQG"
     gene            complement(15145..15594)
                     /locus_tag="VFC01_27250"
     CDS             complement(15145..15594)
                     /locus_tag="VFC01_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZN83347.1"
                     /translation="MTILDTREIQSAPVVRPGLRPAHARRRPRSNRPGGAPLHYRGSG
                     VLMSRASHRRRPITPATTVVLALVAAGITVWLGLVAQFGGVTGGDAPVPSRLAVIQVQ
                     TGETLQQVAHRVAPDAPVGQVIERIRELNQLDSVALDAGQTLIAPIA"
     gene            15805..16509
                     /gene="lexA"
                     /locus_tag="VFC01_27255"
     CDS             15805..16509
                     /gene="lexA"
                     /locus_tag="VFC01_27255"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZN83348.1"
                     /translation="MSDDISDQREGSEGTGGRRLDTALTERQRTILDVIRASVNSRGY
                     PPSIREIGDAVGLTSTSSVAHQLRTLERKGYLRRDPNRPRAVDVRGADDSVTPIVATD
                     VAGSDALPEPTFVPVLGRIAAGGPILAEEAVEDVFPLPRELVGEGSLFLLRVVGDSMV
                     DAAICDGDWVVVRQQNVADNGDIVAAMIDGEATVKTFKRTRGQVWLMPHNPAFEPIPG
                     NDAAVLGKVVTVIRKI"
     gene            complement(16514..18439)
                     /locus_tag="VFC01_27260"
     CDS             complement(16514..18439)
                     /locus_tag="VFC01_27260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83349.1"
                     /translation="MSGQPTVRRTMLGRLAIGLLTVAAVGLMAPVASATPESDADAAI
                     TAAWQAAGGDTGPLGPKDGGVYPAGDGFGQNFPGGKIFFTPAAGAHVMTGAILDKFMS
                     LGGPADGDMGFPTIDEGAGKAPNSRNTTFSAADNPVIFWTPDTGARVVRGPINAAWDK
                     LGGSAGQLGVPADDETYRGNLVTQKFTGGELTYDRKAKTFTTVPPELAGQLVGLQIPD
                     DPVSAINAARRAAGGPLGPLGAAQGAPYSIGADGLGQNFSGGKIFYSPATGANVVTGQ
                     VLAKYESVGGPQGDLGLPTSSEVDGGLATESRMSTFAAKDQPVIFWTPDYGAVIVRGA
                     MNAAWQKLGGATGSLGAPMADQTENGNVITQRFSGGVVSWDRSKNGFSTDPPNLASQL
                     AGLQVPGQEAPKAPPTSPQASDTNDKKWFTWSWWWLLAIIPVVVLGGLVAFAALRNRR
                     RGRDDDLFDTADDGFGHGLPPSYDAAGPLASDDRDTALFGDGYPREGLGGSAPSGAVT
                     AASVWGGLPEHTDEADTAQEPAATPETVVEQEAEDPDTVDTTPTRMPTREERDPLTDT
                     GRHARIVIDEPAPNGTALHLPLDDPNEAPDGYPIKADTKSGLYWVPGSTLYDDARAEI
                     WFASEELARTNGFVRAN"
     gene            complement(18608..19768)
                     /locus_tag="VFC01_27265"
     CDS             complement(18608..19768)
                     /locus_tag="VFC01_27265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN83350.1"
                     /translation="MGSVVKYDRTLFEPEHDLFRESYRAFLDRHVAPYHEQWEQEKIV
                     DRGVWLEAGKQGFLGMAVPEEYGGGGNPDFRYNVIITEETTAGRYSGIGFGLHNDVVA
                     PYLLRLANDEQKARWLPQFCTGEIITAIAMTEPGTGSDLQGIKTRAVKEGDHYILNGA
                     KTFITNGINADLVIVVACTDPDKGAMGFSLLVVERGMEGFERGRKLDKIGLDAQDTAE
                     LSFTDVKVPVENLLGEEGQGFIYLMQNLPQERIGIAVMAATAMEAVLEDTLQYTKERK
                     AFGRPIGSQQNSRFLLAELATEATVVRMMVDEFIKLHLDEKLTAEQAAMAKWYSTEKQ
                     VYLIDRCLQLHGGYGYMREYPVARAYLDSRVQTIYGGTTEIMKEIIGRSLGV"
     gene            19982..22270
                     /locus_tag="VFC01_27270"
     CDS             19982..22270
                     /locus_tag="VFC01_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN83351.1"
                     /translation="MSHAPTSLTHWGGFSAAVASGDIASVMPLDGDTDPSPLLGNLPG
                     AIRHRSRIATPAVRRGWLRDGPGPTTRRGADEFVAVSWEELTELLAGELRRVVDIHGN
                     EAIYGGSYGWASAGRFHHAQSQVHRFLKLLGGYTFSRHSYSLGATGVIMPRVVGTHHD
                     LFKRSTAWEVIVEHTDLLVCFGGLALKNTGINHGGTTGHPARDALRRFRDRGGHIVSF
                     SPLRDDVEGECEWHAPVPGTDVAIMLALAYVLATESLADKDFLNTYCTGYDRFERYLL
                     GLDDGVAKSPQWAASICGLRADDLITLARRMAARRTLVTVSWSLQRIRHGEQAPWMGL
                     TLAAMLGQIGLPGGGFGHGYGSMNEPGLPPLRFGLPRLPQGPNPVQTFIPVAAISDML
                     LHPGEPFDYNGQRLRYPDIKLVYWAGGNPFHHHQNIPRLRRALGRVDTIVVHDPYWTA
                     MAKHADIVVPSTTPYERDDYSGSRNDPLLMAMPKLAEPYAQSRDDYTTFAALAEALGF
                     GEQFTEGRTARQWLTHMYDKWAAGLDFAVPTFDEFWAMGRLRLPTENGLTLLADFRAD
                     PQTHRLATASGRIEVFSEDIDGFGYDDCAGHPSWFKPSEWLGGPRAADYPLHLLANQP
                     ATRLHSQLDIGKVSIASKVQGREPIRMHSADAKFRGLVDGDVVRVFNDRGACLAGVVV
                     DDRLRRDVVQLSTGAWFDPADPADPDAMCVHGNPNVLTDDAGTSSLARGCIGAHVLVQ
                     VEKFTGPMPPVRAHEPPLIVDR"
     gene            22267..22770
                     /locus_tag="VFC01_27275"
     CDS             22267..22770
                     /locus_tag="VFC01_27275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83352.1"
                     /translation="MTEALAVEERGRTITFTFDDMMRYHGGHSPAGVAMAFKVMQRSF
                     AMLSPNGPPERRSITVRTAFRGPGARDGFEAVTRAVSDGRYIVDRTLVRTDRGRRLED
                     FVFVIEIGRHNVTLFLRDGFVTDEFVDLARTENRSDAQEQRLDELKAQLAQRVLGAQA
                     EELFDAV"
     gene            22860..23099
                     /locus_tag="VFC01_27280"
     CDS             22860..23099
                     /locus_tag="VFC01_27280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZN83353.1"
                     /translation="MSEKNSGPEEAIKGVVEGVKGKVKEVAGAVAGRDDLYREGQAQQ
                     DKADAQRNAAQKEAEAESARAAAKTAEKRQEAEQR"
     gene            complement(23107..>24524)
                     /locus_tag="VFC01_27285"
     CDS             complement(23107..>24524)
                     /locus_tag="VFC01_27285"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN83354.1"
                     /translation="DLVAQKDCFVIERLRAAGAILIGLTNMPPMANGGMQRGLYGRAE
                     SPYNAEYLTAAFGSGSSNGSGTATAASFCAFGIGEETWSSGRAPASNNALCAYTPSRG
                     VISTRGGWPLVPTMDVVVPHARTMADMLEVLDVIVADDPRVRGDFWRSQPWIAIPRPS
                     DLRPASYPQLRPTSREEARQVLAGRRFGVPRMYINADPDAGTAEPAGIGGPTGQRIQT
                     RPSVLGLFERARRDLEAVGAEVIDVDFPVVSNYEGDRPGAPTIFTRGLVSPEYLAKEL
                     LDLSAWAWDDFLDDNGDQALNRLADVDGSRIFPHPEGALPDRYTGFDDDIASYPAHVR
                     DHPIESFSDIPHLEEGVRGLEETRRVDLDEWMDGLGLDAVVFPAAADVGPADMDVNES
                     SADLGWRNGVWVANGNLAIRHLGIPTVTVPMGTMDDTGMPVGLTIAGRAYDDVALLRL
                     AAAFEATGARRAAPPRTPPLR"
BASE COUNT         4249 a         8148 c         8001 g         4126 t
ORIGIN      
        1 ctgctcaacg cggaggaaga ggtcgagctg gccaagcgga tcgaggccgg cctctacgcc
       61 acgcagctga tgctcgaatt gaccgagaag ggcgagaagc tgcccgccgc gcaacgtcgc
      121 gacatgtcgt ggatctgccg cgacggcgac cgcgccaaga accatctgct ggaggccaac
      181 ctccgcctgg tggtgtcgct ggccaagcgc tacaccggcc gcggcatggc gttcctggac
      241 ctcatccagg agggcaacct cggcctgatc cgtgcggtcg aaaagttcga ctacacaaag
      301 ggttacaagt tctccacgta cgcgacctgg tggatccggc aggcgatcac ccgcgcgatg
      361 gccgaccagg cccgcaccat ccgcatcccc gtgcacatgg tcgaggtgat caacaagctc
      421 ggccgcatcc agcgtgagct gctgcaggac ctgggccgcg agccgacgcc agaagagctg
      481 gccaaggaaa tggacatcac gccggaaaag gtgctggaga tccagcagta cgcgcgtgaa
      541 ccgatctcgc tggaccagac catcggcgac gaaggcgatt cccagctcgg cgatttcatc
      601 gaagactccg aggccgtggt cgcggtcgac gcggtgagct tcaccctgct gcaggatcag
      661 ctgcagtcgg tgctggagac actgtcggag cgcgaggccg gcgtggtgcg gctgcggttc
      721 ggcctcaccg acggccagcc ccgcacgctc gacgagatcg gccaggtcta cggcgtcacc
      781 cgcgagcgca tccgccagat cgagtcgaag acgatgtcaa agctgcgcca ccccagtcgt
      841 tctcaggtgc tacgcgacta cctcgactag cgaagttacc gcggctggtt tcggagcacg
      901 ccaccaattg gcaatgcgga tcgcgcatta gccctaaggt gcggctcatg cgggtgtccg
      961 ataacgcact cagaaagctc atcaagggaa cgttcctcgc cactctccta ctggctgcga
     1021 tggagggcgg cacgccactc cacgaagcga cgtggatttt cgtgggtgcg gttttgacca
     1081 cggtcgtcga cgcatacgca acacacatgt ctggtcgcca cgagggtggt atgccggcat
     1141 acttcggcag cctgtatcgc ggcgtcgtgg gcgatctgcc gcgggttatt ggctctctgc
     1201 cgaccgtgct gttgctcgtc ttcgcgtgga tgttccaatg gcaccacgat ctccggaacc
     1261 ccgacggcag tgtgtcagtc ggctacgaaa ccgtcgcgct gaacgtcaac gtcgtgttgt
     1321 tgttcatctt ctcgatactc gccgcatacc ggagcggtgc atcgtggcgc ggaaccgtta
     1381 tcttcgcatt gatgaacgcc gccttgggct ggatcatcgt cacggtcgag cttgcgctcg
     1441 agtagttttc agctcgttgc tggcgtgcgc tagcatcggc cagcgcccgg cagaatgcct
     1501 gtcgggcacc gatattcgag tgccccgcga aggagcgatt tgtgtcaacg gcagagaaac
     1561 caccgcggat cccgccgctg gcgctggttc gcgccatcaa cgccgtccgg gcgggcgccc
     1621 agcgggtgag tcgtgcgctg gcccccggcg gactcaacgt gatggagctg ctcaccgggg
     1681 cgtggacggc gcaaaccatc tacgtggcgg tcaagctggg gattcccgac gagctcgccg
     1741 acggcccact gcccgccgac gaggtggccc gccgggtcgt cgcggacccc gatgcggtct
     1801 accggctgat gcgggcgctg gcgtcccggg gcgtgctgcg tcaccgcacc gacgacacgt
     1861 tcaagctgac gtcgatcggc aaggcgcttc gcactggcac cccgggctca atgcgcgaca
     1921 tggcattgtt ccttggtcat ccgctgcgct gggaggactg gggcaacctg ctgtactcgg
     1981 ttgaaaccgg aaagccgtcc gtggaaaagc tgcgcggcat gccgttcttc gaatacgtcg
     2041 agaccgacac cgatctggcc gaagcgttca acaacgcgat gacagcgggc agcgagttct
     2101 ccatctacgc ggtgctggcc gcctacgact tcagcggtta tcggaagatc atcgacatcg
     2161 gtggcggcca cggccggctg ctctcgatga tcctggcgag ggccccacac gctcgcggcg
     2221 tgctgttcga cctccccaac gtcgtggacg gtgcaacgga ggagctgacg aaagccggtg
     2281 tggcagagcg gtgtgacgtg gtcggcgggt cgttcttcga cgccgttcct gagaacggcg
     2341 acgcctacct catgaaggcg attctgcacg actggagcga tgaggacgcg ctgaagatcc
     2401 tgagcacgat ccgcgccgcg atcgcacccg gcggcaagct ggtcctactg gaaagcgttc
     2461 tgccggaacg ttcttcgtcg gacatcggcc tgctgatcga cctcgagatg cttgtcgccg
     2521 tcggcggcag ggagcgtacg cacgcggagt gggcgaatct cttgggccgg gccgggttcc
     2581 ggctgagccg ggtcgtgcgc acggcaacac cggtgtcgat cgtcgaggct gtcccggcct
     2641 agcgcttctt gtcgcgcacc ttgtacgtcg agggccacga ctcgcgcgtc tcgtcgccca
     2701 cgagtggaac gcccttgctg ccgatcctga tgtcgtaggg ttccttgccc gcgccgacct
     2761 cgcggttggc gtgctcggtg gccagataga tcagttgatc gatctcggct tcggcgaagg
     2821 cgacgaacgt ggtcttgcgc acgatgtcct cgggcggcgg cgtcatcttc tcgggcagga
     2881 cccgcgaagc aagtgccgca aggcccagca acgagaacgg gatgacccag tagcagacga
     2941 acgacagtgg agtcttggtg tcgaggatcg tcgcctcgcc atacacaatg ggcgggatcg
     3001 ccgaggacac cgtcataccg gcgaacgcgg cgacccagat ccaggtcaac agcgtggtga
     3061 tccgttggaa cagttcgctc tcgaccacct ctttcggctg gtcgacttcg gcgaactcac
     3121 gcacgaacgg tctgccggcg agcacaccga caaacgcgac gaggaagatt ccggcattgc
     3181 tcagcggctg catccaccgc tccatgaacg actggctcag tgtgaacgtc aacaccgtca
     3241 gaatcaaaaa cgttgccaca gcgccgattt cgagggtacg cccaggcgcc cccctgaccc
     3301 gaccgatcgc gaaagacagc acggcgaccg cgagcgcgac cagtaccgcc gtcgtgaacg
     3361 gaacattgcc gacaagcacc caatagacga tccacggcgc gaagcccaac agaatgccca
     3421 cgaagggtca gtctaggttg tcagtcaacg cccgaactgt aaacctcttg catggtcgat
     3481 atccgcggct agtttgaacg gatggcgcag cgccgcacgg tgtcctcggg ctcggagtac
     3541 gagtcggccg ttggctattc gcgtgcggtc cgcaccggcc cgcacatcgt cgtagccggt
     3601 accaccggac agggccacgg tatcgccgca cagacacgag aggccttgcg ccgcattgat
     3661 ggtgcgctcc atcaactcgg cgcgtcgttg tccgacgtgg tgcgcacgcg gatctatgtc
     3721 accgaaatct ccctgtggca ggaagtcggc gcggtccacg ccgaagtgtt cggcgagatc
     3781 cgtccggtca ccaccatggt cgaggtctcg gcgctcatct cgcccgacct gctggtcgag
     3841 atcgaggtcg acgcgtacct cgacgcctag agcggcgcga accgtccgcc ctcgtcaggc
     3901 cggtacggcg tgacgtttat cacagcttcc aggggcaatg ctccgtacag gtgcgggaac
     3961 accatggcat cgggatcggt ggcgacccct ggttcccacc ggacgggaga tgtcaggcgt
     4021 gccgggtcga tgtgcaacag cacaagatca gtccggccgg cgtagagccg gttggccggc
     4081 agatgcacct gttcagtcgt cgacagatgg acgaagccgc attcatccag cgaatccggg
     4141 cgatgctcgc cgcgggcctg cgccgagcgc cattcgtcgt tgctgcagag atggagcaag
     4201 acgctcgggg ctgcatacat accgccagcc tgcactgctc gcaaatccac gcaagcgtga
     4261 gacacgacac acccgtaaac ggccggggaa caaggccgga accgaaaacg tctgagacag
     4321 tggatgtacg cgaagcatta cggaggtcgc catgaacgca acgctcacca gtcccgaact
     4381 gacgagggct gaccgctgcg atcgctgtgg agccgccgcg cgcgtacgcg cgaagcttcc
     4441 gtcaggctcc gagttgctgt tctgccagca ccacgccaac gagcacgagg ccaagctggt
     4501 cgaactcgcc gccgttctcg aagtcagccc gctggagccg tagcaccgaa taggcaatgc
     4561 tggtctggtc atggatgacc agtcccagcc gataccggtg ccgccgtcgc gtcaccacat
     4621 ccggcgtctt gttcgtcgga cgctgtcgaa gagttgggac gactcgatct tttcggagtc
     4681 cgcccaggcg gcgttctggt gtgcgctctc cctgccaccg ctgctgctgg ggatgctggg
     4741 cagcctggcg tatatcgcgc cgctgttcgg cccggacaca ctgccgacga tccaggagca
     4801 gttgatcagc acggcaggcc ggttcttctc ccccaacgtc gtcaacgaga tcatcgcgcc
     4861 gacggtccgg gacatcgtca agggcgcccg cggcgaggtg gtgtcgatcg gcttcgtcat
     4921 ctcgctgtgg gccggctcgt cagccatctc ggcgttcgtc gactcgatca ccgaggccca
     4981 tggccagaca ccgctgcgtc acccggtgcg ccagcggttt tacgcgctcg gcctgtatgt
     5041 ggtgatgctc gtaggggcga tcgtgacggc gccgttcatc gcgctggggc cgcgcaagat
     5101 cgccgagtat cttccggata gttgggacag cgtgctggcc tacggctatt acccggtgct
     5161 gttcgtgggc ctggtggtgg cgatgaacgt cctctaccgg gtgtcgctgc ccaaaccatt
     5221 gccgtcgcac cgtcttctga tcggctcggt gctggcgacg gcggtgttcc tgatcgccac
     5281 gtacggactg cgggtgtatt tgacgtggat caccaggacc ggctacacct acggcgcgtt
     5341 ggcgacgccg atcgcgttct tgttgttcgc gttcttcctc ggcttcgcga tcatgctcgg
     5401 cgccgagttg aacgccgcga tccaagagga atggccggcg gcggcgaccc atgcgaagcg
     5461 gttccggtgg tggctggagg aaaaggcaga gtcactcaac ggcaacggtc ccgtgatcac
     5521 gcagccggcg gctaccgccg ccgatggcga cgctacttct tgagcttttc gtagatccgc
     5581 ttgcactcgg ggcacactgg ggagcccggc ttggcggccc gcgtcaccgg aaacacctcg
     5641 ccgcacaacg cgacgacgtg cgtacccatg acagcgcttt cggcgatctt gtccttcttc
     5701 acatagtgga agtacttcgg ggtgtcgctg tcggtcccgt cgtcgacgcg ttcgtcggtg
     5761 tcggtgcgct cgatcgtctg ggttcgcatg tgacccattt tgcccgctgg taatccggcg
     5821 cgtacagcgg acgacgactg tggaacagtg gagatatgaa acacggccaa gagctgagtt
     5881 tcgacgacga gggccggccc gtcctgatca ctcgcgcagc ggcaccctat gaggagcagc
     5941 accgcgagcg ggtgcgcaaa tacctgacac tgatgttctg gcgcatcccg gcgctgatcc
     6001 tcgctgcggt cgcgtacggc atctgggaga acgggctcat ctcgctcgcg atcattctcg
     6061 tatcagtgcc cctgccgtgg atggccgtgc tgatcgccaa cgaccgcccg ccgcggcgcg
     6121 ccgaggagcc tcgacgctat gactcacgcc gccggatacc gctgtttccg acggccgagc
     6181 ggcccgctct ggagcgcggc ccccattcgg caccgcaacc ggacggcggc gcctcgcccg
     6241 gcgatgttcc cagctgagga acttctggct ctattctcag cacattctca ggtcgagcgc
     6301 ggataaccgc agatcagaag gtgtgaaccg gccgacgcgc gggaactctt agcgggtcgt
     6361 gggcgttgta gctcatgaca gttcgacccg accaggaggc cgtcatggca aatgccacca
     6421 ctacccgcgt cgaccacgac ctggacgccc agagtccggc cgcggacctc gtacgcgtgt
     6481 acttgaacgg cattggcaaa acggccctgc tcaacgccgc cgacgaggtc gaactcgcaa
     6541 agcgcattga ggcaggcctt tacgcgcagc atctgctgga aacgcggaag cgcttgggcg
     6601 agaaccgcaa acgtgatctt gctgcggttg tccgcgacgg tgaggcggcc cggcgccatc
     6661 tgctggaggc caaccttcga ctggtggtct cgttggcgaa gcgctacacg ggtcgcggca
     6721 tgccgttgct ggacctcatt caggagggca acctgggtct gatccgcgca atggagaaat
     6781 tcgattacag caagggattc aagttctcga cgtacgccac ctggtggatc cgtcaggcga
     6841 tcacccgcgg catggccgac cagagccgca ccatccggct gcccgtccac ctcgtcgagc
     6901 aggtgaacaa gctggcccgg atcaagcgtg agatgcatca gaacctgggc cgggaggcca
     6961 ccgacgagga gctggccgcc gaatccggca ttcccgtgga gaagatcaac gacctgctcg
     7021 agcacagccg cgacccggtg agcctggaca tgccggtggg tagcgacgag gaagccccgc
     7081 tgggcgactt catcgaggat gccgaggcga tgtcggccga gaacgcggtc atctccgagc
     7141 tgttgcacac cgacattcgc cacgtgctgg caaccctcga cgagcgcgag cagcaggtca
     7201 tccggctgcg gttcggcctc gacgacgggc agccgcgcac cctcgatcag atcggcaagc
     7261 tgttcggctt gtcccgcgag cgtgtgcgcc agatcgagcg cgaggtgatg gccaagctgc
     7321 gcaacggcga gcgcgccgac cggcttcggt cgtacgccag ctagctgagc atcgacgacc
     7381 ccgccaggag tttccggcgg ggtcgttgct gtgtgcggct cagttttccc agcttgaccg
     7441 gtagactctg gcgtgacgga gggttgccta atgaacgatc ttgtcgatac caccgagatg
     7501 tacctgcgga ccatctacga cctcgaagaa gagggcgtag tgccgctgcg tgcccgcatc
     7561 gccgagcgcc tcgagcagag tggccctacc gtcagccaga ccgtgtcccg aatggagcgc
     7621 gacgggctgc tgcacgtcgc tggagaccgc cacctggaac tgaccgacaa gggccgctcc
     7681 cttgccatct ccgtgatgcg caagcatcgc ctcgccgagc ggctgcttgt cgacgtgatc
     7741 gggctgccgt gggaggaagt gcacgccgag gcatgccggt gggagcacgt gatgagcgag
     7801 gatgtcgagc gcaggctggt gcaggtgctc aacaacccca ccacatcccc attcggcaat
     7861 cccatcccag gcctgtccga gctcggcgtg ggcgacgatc tcgccagcga ccagatcaac
     7921 ctggtgcggc tcaccgagct gccgtccggg atgcccgtcg cggtcgtcgt gcgacaactc
     7981 accgaacacg tccagggcga cgccgaattg atcgcgaagc tcaaggacgc cggcgtggtg
     8041 cccaacgcca gggtgacggt cgaggtcaac gaccacggcg gcgtcatgat cgtgatcccc
     8101 ggccacgaac aggtcgagct gccccaccac atggcgcacg ccgtcaaggt cgaaaaggtc
     8161 tgacccgctg ggcccacacc gtaggttggc ccacaccgca ggtcggggaa ggacactatc
     8221 ccgcccggcg tccgaacgac cgcatgggcg gtaactgcac gccgagccga tcggccagcc
     8281 ggtaacccgt gatcgccaga tcacgaatct tctcgggccg aagccccgat tgcagtgcgg
     8341 tgtccagcat gcccgccatg cggtgcgtcg gatcgccgcg cagcgccgct tccagcgaca
     8401 cgccggccag cggaccgtcg ccacgcgcgt aggccgagaa cgccaacaag accaacgcct
     8461 cgacccgcca cggttccggc agcgtgcgcg acagcagcgc ccacagcgac tcggcctgtc
     8521 cggcgttctc gccgaccgcc agcgcgtaga gcgtgtcccg cacccgcaga tccgtcagcg
     8581 cccacgccag ccgcgccagt tcgtcgtcgt cgagttccac agcgtcggcc acccgcgctg
     8641 ccgcggcgat ggtcgcctcg atgtcggcac gcgccgcctc gtccggccgc tccgccgaat
     8701 ccggtttgcg gtccccgatg atctcggcga gcaggtcggc gcgagcggaa ttggccacct
     8761 cgataacctc ctggagctcg gcgcgtcgcg catacagccg acggccgtcc aggaccgcgg
     8821 cgacggccag cggcgatgcc gacgggtcgt cgactgtgcc cgcattgccg cacccgtcag
     8881 cgcaatgcca ccgcccgccg gcggcgaccc ggtcgacgac gtgcgccgcc agcagctcga
     8941 tgctccgatt ggccaacgcc gtcgtcaggc ggtcggccag ctcgcggtat tcgtcgttgc
     9001 acatccggca gctcgcgccg tcctcgtcga tcacaaccgc gatcgccgaa tcgggtttcg
     9061 ccgccgcggc cacatccgcg atatgcgcca gcgagtgctg cagttcgtcc gaaaggtcaa
     9121 cgcgcataac gcatcccatt tcgccgcggt cgacagtcac gagcacgaga gatttctccg
     9181 gcacgaaacc gaggacggcg ggcagggccg cgatcagaac gccaggccgg ttgaggtgga
     9241 agtcgggtag ttgcttcgtc atggcgccaa cctcgcagcg ggcactgccg gattcagccg
     9301 caaagatcgt cgtccgaagg cacctgggga tgagttcggc actggggata acccaaagga
     9361 acgccgacga gccaattatt cacgcggcgt tgacgcccgt cggcgtgcga atttgcccga
     9421 accggcgtag accttttgcc atgggttcga tggagtacga cctcgtcgtt atcggttccg
     9481 gtccgggcgg ccagaaggcc gcgatcgcgg cggcaaagct cggcaagtcg gtcgcggttg
     9541 tcgagcgcgg caggatgctg ggcggcgttt gcgtgaacac cggaaccatt ccgtccaaga
     9601 cgctgcgcga ggcggtcgtc tatctcaccg gcatgagcca gcgcgaactg tacggctcaa
     9661 gctaccgcgt caaggagaag atcactcccg gcgacctgct ggcgcgcacg acacacgtca
     9721 tcaacaagga gcaggacgta gtgcgcaatc agctgatgcg caaccgggtt gacctccaca
     9781 ccgggcacgc ccggttcctc gacgagcaca ccgtgtgtgt cgatgaccag acccgcggtg
     9841 aaagaactac cgtccgcggc gagtacatcg tgatcgccac cggcaccaag ccggcccgcc
     9901 cgaccggcgt cgaattcgac gaagagcgcg tgctggactc cgacggcatt ctggatctga
     9961 attcgctgcc ggccacgatg gtggtggtgg gcgccggcgt gatcggcatc gagtacgcct
    10021 cgatgttcgc cgcgctgggc accaaggtca ccgtcgtgga gaagcgcgaa aacatgctgg
    10081 acttctgtga tcccgagatc gtcgaggcgc tcaagtttca cttgcgcgac ctggcggtga
    10141 cgttccgctt cggcgaggag gtgaccgcgg tcgacgtcgg ctccgcgggc acggtcacca
    10201 cattggccag cggcaagcag attcccgccg agaccgtgat gtattcggct ggccgccaag
    10261 gccagaccga acacctggat ctgaccaatg ccggcctcga aaccgacggc cgcggaagaa
    10321 ttgtcgtcga cagccagttc cagacgaagg tcgaccacat ctacgccgtc ggcgacgtga
    10381 tcggcttccc cgcgctggcc gccacgtcga tggaccaggg tcggctggcc gcgtaccacg
    10441 cctttggcga accggcgaag ggcatgaccg atctgcagcc gatcggcatc tactccatcc
    10501 ctgaggtgtc ctacgtcggc gccaccgagg tcgagctgac gaagaatgcg attccgtacg
    10561 aggtcggggt gtcgcgatat cgcgagctgg ccaggggtca gatcgcaggc gactcgtacg
    10621 gcatgctcaa actcttggtg tcgaccgacg acttaaagct gttgggtgtc cacatctttg
    10681 gcacgagcgc caccgaaatg gtgcacatcg gccaggcggt gatgggctgc ggcggcacgg
    10741 tcgaatacct cgtcgacgcc gtcttcaact acccgacgtt ctcagaggcc tacaaggtcg
    10801 ccgcactgga tgtgatgaac aagctgcgcg cgctgaccca gttccgggcc taggcccttt
    10861 agtcgcagtc gtcgtcgaac accgtcggtg cagcgtcggc cgggccgctg gcttcggccc
    10921 gggtgaataa ccgtttgatc gcatcgtcga aaggcggtga gtacgagaca ctttcgtctt
    10981 cgcagccgcc gcccttcaac ccgccgatca gcccggtgac ccgcgatccc tcgatccacg
    11041 gcgcgcccga caggccgttg accagttctg cgcagtccag cgccgggaag ccctgcgccg
    11101 gtggcgccgt cgccgtccgg cagccgatcg gaccgccacc gtcgcccatc gcatagccgg
    11161 tgacgtcgac gacggtgccg ggtttcgggg caggcccgat cgtcaagcca ctgccggcct
    11221 gcgcctgcac cgatccaccc gagtcccggc tcacccgtgc gatcgcgaag tcggccagcg
    11281 ggtcctggtt ctgcacccag cgcggatcga gatagaccgc gtcgatgtgc cagacgtccg
    11341 ccggtgcggc ggcgtccttc aggccagcaa cgaacgtggc gtcgacgcct tcggcgacgc
    11401 aatgcgcggc ggtgaggatg aggtcaccgg tcgccgaatc caggaccgcg ccgctacagg
    11461 tgtgcatcgt cccggcgccg aggaacaccg cgccgacgcg cggatcggac gggaccggct
    11521 gggcggtggg cggcgccttg gcctcgactc tggcggcctg ttccaccgga ggatgcttcg
    11581 cgcacgaggg gctcaacgca accagcagtc ccgttgctag gacggtcaac ctggccgcgg
    11641 agaagcgcat gacgtccgat cctgccccaa ctcggaggtg tgcgccctga cgattgcctg
    11701 caagccgggt cgcaatcgca tccagcggga aatgcgcgac actaggtgcc aggaggcaac
    11761 gcaatggctg agaacgcgga tcgaccagaa catcactacc agcccgaaca gaccgggatg
    11821 tatgagctgg agtttcccgc gccgcagttg tcatcagccg atggcagcgg gccggtgatg
    11881 atccacgccc tggagggctt ttcagacgct ggacacgcga tccggctggc cgcgcagcac
    11941 ctgaagaacc acctggacac ggagctggtt gcgtcgttcg ccatcgacga actcctggac
    12001 tatcgctcgc gacggccgtt gatgaccttc aagacggacc acttcacgtc ctacgaggaa
    12061 cccgagctca acctctacgc cctgcacgac agcgtcggaa ccccgttttt gctgctcgcc
    12121 ggcatggagc cggacctgcg atgggagcgg ttcatcacgg ccgtacggct gctggccgaa
    12181 cggctggggg ttcgccgggt gatcggcatc ggcaccatcc cgatggcggt gccgcatacc
    12241 cggccgatca cgatgacggc acacgccaac gacaaggagc tgatcgcaga ccacacccca
    12301 tgggtgggtg aagtggtggt gccgggcagc gtgtcgaacc tgctggagtt ccgaatggcc
    12361 cagcacggcc acgaagtagt cggcttcacc gtgcacgttc cgcactacct ggcgcagacc
    12421 gattacccgg cagccgccca agcactgctg gcggaggtcg ccaagagcgg ctcgctgcag
    12481 attccgctga atacgctgac gcaagccgcg gcggaagtgc acgacaagat caacgaacag
    12541 gtcgaggcca gtaccgaagt cgctcaagtg gtggaggccc tggagcgaca gtacgatgcc
    12601 ttcgtcgccg ctcaggagaa ccggtcactg ctggcgcgcg atgaggatct acccagcgga
    12661 gacgagctcg gcgccgagtt cgagcggttc ctcgcacagc agaccggtga caagcccaac
    12721 gacgacgacg acaacaaata gcagccctcc gacaacgaag ttggctcaat gacagcgcga
    12781 aagcccaacc tgcgccccgt gcgggaactc acaccaacgc tgcagttccg caccatccac
    12841 gggtataggc gcgcgttccg ggtggccggc tccggcccgg cgatcctgct gatccacggg
    12901 atcggtgaca actcgaccac gtggagcacg gtgcagacca agctcgcgca acggttcacg
    12961 gtcatcgctc ccgacctgct gggtcatggc aagtccgaca agcctcgggc cgactactcg
    13021 gtcgccgcct acgccaacgg aatgcgcgac ctgttgtcgg tcctcgacat cgacaacgtg
    13081 accgtcatcg gtcattcgct cggcggcggc gttgcgatgc aattcgctta tcaattcccg
    13141 caattggtcg atcgactgat cctggtgggc gcaggcgggg tgaccaagga cgtgaacgtc
    13201 gcactgcgga tcgcctcact gccgatgggc agtgaggccc tggcgttctt gcggctgccg
    13261 ctcgtgctgc cggcgatgca ggttgtcgga cgcgtcgcgg gcgcggtgtt tgggtcgact
    13321 ggcatgggcc gcgacctgcc cgatgtgctg cgcattctgg ccgacctgcc cgagccgacc
    13381 gcctcctcgg cctttgcccg cacgctgcgc gccgtcgtcg actggcgcgg ccaggtggtc
    13441 acaatgctcg accgatgtta tttgacgcaa tccgttcccg tgcaattgat ttggggcagc
    13501 cgcgattcgg tgatcccggt aagccacgcc cggatggcgc acgcggcgat gcccgggtcg
    13561 cagttggaga tcttcgaacg gtccgggcat ttcccgttcc atgacgatcc tgaccgcttc
    13621 gtcgaagttg tcgagcggtt catcgggtcc accgatcccg cggtgtacga ccaggaatat
    13681 ctccgcagcc tgctgcgcgc cgggatcagt gaaagcacga tcgccgggcc tgtcgacacc
    13741 agggtggccg tcctcgatgc gatgggcgcc gacgagcgca gcgccacctg attggagacc
    13801 acgtagcatc ggccacatgg ccatcgacgt gacagtgctg cgcgttttca ccgaccagga
    13861 cggcaacttc ggcaaccctc tcggcgtcgt ggatgccagc caggccgagc ccagcgagcg
    13921 gcagcggata gcaactcaat tgggttacag cgaaacgata ttcattgacg tgcccagggc
    13981 cggagccagc acggcgcacg cgcgcatcta cacgcccgcc accgagctgc cgttcgccgg
    14041 ccacccgacg gtgggcgctt cctggtggct acgcgaccgc ggcacgccag tccgtacgct
    14101 gcaggtgccc gcgggcatcg tccaggtcgc gtacgaggac gacctcaccg cggtcagtgc
    14161 ccgctcggac tgggcgccgg agttcgctat ctacgacctc gactcgacgg atgacctgtt
    14221 cgccgcgaac ccggacgagt atcccgatga catccagaac tatctgtgga cgtggaccga
    14281 ccgcgagcgg ggtgcgattc ggtcgcggat gttcgcggcg aatctcggcg tgcccgagga
    14341 cgaggcgacc ggagcggcgg cagtgcgaat caccgactac ctcagccgcg acttgaccat
    14401 cctgcagggc aagggctcgg tgatcgaaac gcagtggagc ccggaaggct gggtccgtat
    14461 cgccggccga gtcgtgaacg acggaaccaa gcaggtcgac tgacgccgac cgatggctca
    14521 gccctgtgcg gggacctctc ggtgcgcgcg cagcgcctcg atctcgcgct cgaaatcctc
    14581 ggccgactcg aacgaccggt agaccgacgc gaatcgtagg taggccacct catcgagttc
    14641 gcgcagtggc ccgaggatcg ccaacccgac ctcatggctg ggcacttcgg gcgagcccgt
    14701 ggcccgcacg ctgtcctcga cctgctgggc gagcagattc aatgcgtcgt cgtcgacctg
    14761 tcggccctga caggcccggc gcacgccccg aatgaccttc tcccggctga acggctcggt
    14821 gacgccgctg cgcttgacga ccgccagcac ggcggtctcc accgtggtga accgtctacc
    14881 gcactcgggg cacgatcggc gccggcgaat ggcctggccc tcgtcggttt cgcggctgtc
    14941 caccacccgg gaatcgggat gacggcagaa cggacaatgc atcaccgctc cttcgccgcg
    15001 acgacaaaca gctacctcga acctcttcga gcgtacctgc gcgcaacccg gcgagcctag
    15061 cgccggtgcg attgaggcca acgccgtcgc tcgctccgcc agagtcgttg cgcggcaata
    15121 tctttcaccg ctcatgacac agggtcacgc gatgggcgcg atgagtgtct ggccggcgtc
    15181 aagcgccacc gagtcgagtt ggttgagttc gcggatgcgc tcgatgacct ggccgacagg
    15241 cgcgtcgggc gcgacccggt gggccacctg ctgcagcgtc tcccccgttt gcacctggat
    15301 caccgccaac cggcttggca ccggcgcatc gcctccggtc acgccgccga actgggcgac
    15361 caacccgagc cacacggtga tcccggccgc gacgagcgcg agcaccacgg tggtcgcggg
    15421 cgtgatgggc cgccggcgat gcgacgcccg cgacatcagg acgccggagc cgcggtagtg
    15481 cagcggcgcg ccgccgggcc ggttcgaccg cggccgccgc cgggcgtgcg caggccgcaa
    15541 tccgggcctg accaccggtg ccgactggat ttctcgggtg tcgaggatcg tcatctgcct
    15601 gcctttcgct cggggcgctt tcgctcttgt gttcgaacat actcgctcac ttgttcgatt
    15661 tatagaacat gtgatcgaac tgttgccaaa cgatagatca gcccaccgac aaatttcggc
    15721 cacgcaatat gcgcagacgt caagaggcga cacgcctcga acacatgttt gattattgga
    15781 tcggctggga ctacattcgg cggcatgagc gacgacatca gcgaccagcg tgaaggctct
    15841 gaaggcaccg gcggacgccg actcgacacg gctctgaccg agcgtcaacg caccatcctc
    15901 gacgtcatcc gcgcctcggt gaacagtcgc ggctatccgc cgagcatcag ggaaatcggc
    15961 gatgcggtcg gtctgacatc gacgtcgtcg gtggctcatc agctgcgcac cctggaacgc
    16021 aagggctatc tccggcgcga ccccaaccgg ccgcgtgccg tcgacgtgcg cggcgccgac
    16081 gacagcgtga cgcccatcgt cgcgacggac gtcgccggct cggacgcgct accggagccc
    16141 accttcgtgc cggtgctcgg ccgcatcgca gcaggcggtc cgattctggc tgaggaagcc
    16201 gtcgaagatg tctttccact cccccgcgaa ctcgtcggag aagggtcgct gttcttgctg
    16261 agggtggtcg gtgactcgat ggtcgacgcg gccatctgcg acggtgactg ggtggtcgtc
    16321 cgtcaacaga acgtcgccga caacggtgac atcgtcgcgg ccatgatcga tggcgaggcg
    16381 acggtgaaga cgttcaagcg aactcgcggt caggtgtggc tgatgccgca caatccggcg
    16441 ttcgagccca ttccaggcaa cgacgcggcc gtgctcggca aggtcgtcac ggtgatccgc
    16501 aagatctaac cgtctagttc gccctgacga acccgttggt gcgagcgagt tcctcactgg
    16561 cgaaccagat ttcggcacgc gcgtcgtcgt agagcgtgct gcccggcacc caatacaacc
    16621 cggacttggt atccgctttg atcgggtagc cgtccggcgc ctcgttgggg tcatcgagcg
    16681 gcaggtgcag tgcggtgccg ttcggcgcgg gctcatcgat cacgatccgg gcatggcgac
    16741 cggtgtcggt caacggatcc cgttcctcgc gggtcggcat gcgagtcggc gttgtgtcta
    16801 ccgtgtcggg atcctctgcc tcctgctcga caactgtttc cggcgtcgcg gccggttcct
    16861 gcgccgtgtc ggcctcgtcg gtgtgctcgg gtagccctcc ccacacgctc gccgcggtaa
    16921 ccgcgccgga tggggccgac cccccaagac cctcccgcgg atagccatcc ccgaacagtg
    16981 ccgtgtcgcg gtcgtctgac gccaacggac cagccgcgtc atacgacgga gggagcccgt
    17041 gaccaaaccc gtcgtcggcc gtgtcgaaca ggtcgtcatc gcgccctcgg cggcggttgc
    17101 gcagcgccgc aaaggccacc agcccgccca ggaccacgac aggaatgatc gcgagcagcc
    17161 accaccagct ccaggtgaac cacttcttgt cgttggtgtc tgaggcctgt gggctggttg
    17221 gcggtgcctt gggcgcctcc tgtccgggaa cctggaggcc tgccagttgc gaggccaggt
    17281 tcggcgggtc ggtgctgaag ccgtttttgg accggtccca ggagacgacg ccgccgctga
    17341 accgctgcgt gatcacgttg ccgttctcgg tctgatcggc catcggcgcg cccagcgaac
    17401 ccgttgcacc acccagtttc tgccacgcgg cgttcatggc gccacgcacg atgacggcac
    17461 cgtagtcggg ggtccagaag atgaccggct gatccttcgc ggcgaacgtg ctcatccggc
    17521 tctcggtggc caggccgcca tcgacctcac tggacgtcgg caagcccagg tcgccctgcg
    17581 gtccgccgac gctttcgtat ttggccaaca cttggcccgt gacgacattg gctccggtcg
    17641 ccggactgta gaagatcttg ccgcccgaga agttctggcc gagaccgtcg gcaccgatcg
    17701 agtacggcgc gccttgcgcg gcgcccagcg gacccaatgg gccccccgcc gcgcgccttg
    17761 ccgcgttgat ggccgacacc gggtcgtccg ggatctgcag gcctacgagt tgtccggcga
    17821 gctcaggcgg caccgtggtg aaggtcttgg ccttccggtc gtaggtcagt tcgccgccgg
    17881 tgaatttctg cgtgacgagg ttgccgcgat acgtctcatc gtcggcgggc acgccgagct
    17941 gaccggccga cccgccaagt ttgtcccatg cggcattgat gggaccgcgg acaaccctgg
    18001 caccggtgtc gggggtccaa aagatcaccg ggttgtcggc ggcgctgaac gtggtgttgc
    18061 ggctgttcgg agcctttcct gcgccctcgt cgatggtggg aaagcccata tcgccgtcgg
    18121 caggcccgcc gagtgacatg aacttgtcca ggatcgcgcc ggtcatgacg tgagcgccgg
    18181 ccgccggagt gaagaagatt ttgccgcccg ggaagttctg gccgaagccg tcaccggccg
    18241 ggtatacacc gccgtccttg gggccgagcg gacccgtgtc gccgcctgcc gcctgccacg
    18301 ccgcggtgat cgcggcatcg gcgtcgctct cgggcgtggc cgacgcgacc ggcgccatca
    18361 acccgaccgc cgcgactgtc agcagaccga tggccagccg tcccagcatc gtgcggcgca
    18421 cggtcggctg cccgctcatc gatcccctcc cgcggtcggc gcaagttact gcgatgtgta
    18481 acgcgtccgt caactctgcg tcagcagacg tcgattacca aggaccatcg ccgatcttgg
    18541 agagaaaata cctgggcagg cgggcctggg ccgctagacg cccaggctgg accagacggt
    18601 ctcggcgcta aacgccgaga ctgcgaccga ttatctcctt catgatctct gtcgtaccgc
    18661 cgtagatcgt ctgcacccgt gagtcgagat acgcccttgc gaccggatat tcgcgcatgt
    18721 agccgtaccc gccgtgcagt tgcaggcagc ggtcgatgag atagacctgc ttctcggtcg
    18781 agtaccactt cgccattgcg gcctgctcgg ccgtcagctt ctcatcgagg tgcaacttta
    18841 tgaactcgtc gaccatcatg cgcaccaccg tggcttcggt cgcgagctcg gccaagagga
    18901 accggctgtt ctgctggcta ccgatgggac ggccaaaggc cttgcgctcc ttggtgtatt
    18961 gcagcgtgtc ctcgagcacc gcctccatcg cggtggccgc catcaccgcg atgccgatcc
    19021 tctcctgggg caggttctgc atcaggtaga tgaagccttg gccctcctcg ccgagcaggt
    19081 tctcgacggg caccttcacg tcggtgaacg acagctcggc ggtgtcctgc gcatccaggc
    19141 cgatcttgtc gagtttgcgg ccgcgctcga atccctccat gccgcgctcg acgaccagca
    19201 gcgagaaccc catggcgccc ttgtcgggat cggtgcatgc gaccacgatc accaggtcgg
    19261 cattgatccc gttggtgatg aaggtttttg cgccgttgag gatgtagtgg tcgccctcct
    19321 tgaccgcgcg ggtcttgatg ccctgcaaat cgcttccggt gccgggttcg gtcattgcga
    19381 tcgcggtgat gatttccccg gtgcagaact gcggcagcca gcgcgccttc tgctcgtcgt
    19441 tggccagccg tagcaggtag ggcgcgacca cgtcgttgtg caggccgaaa ccgatgccgc
    19501 tgtagcggcc cgctgtggtc tcctcggtga tgatcacgtt gtagcggaag tcggggtttc
    19561 caccgccgcc gtactcctcc ggcaccgcca tgcccaagaa gccctgcttg ccggcctcga
    19621 gccagacgcc acgatcgacg atcttctcct gctcccactg ctcgtgatag ggcgcgacgt
    19681 gccggtccag gaatgcgcga taggactcgc ggaacagatc gtgctcgggc tcgaagaggg
    19741 tgcggtcgta tttgacgacg ctgcccatgg ctgacctcca tgaagctggt acgggctgtt
    19801 accgccaacg taccccatcc aaccgggtgg ttggttgggg gcccgcgtgt cgcccagcgt
    19861 gtcgccagaa ggagaacgtc tccggccggt cctagacttc gcggggtgac ctccgaagac
    19921 gacaacgagt gaggatcgca gcgaggcacg agcgaggacc ggagcgagga ggagtcgagg
    19981 catgagtcac gccccgacga gcctcactca ctggggcggc ttttcggcgg ctgtcgcatc
    20041 cggcgacatc gcttccgtga tgcccctaga cggcgatacc gacccctccc cgctgctggg
    20101 caacctgccc ggcgccattc gccatcgctc ccgcatcgcg acgcctgcgg tccggcgcgg
    20161 ctggctgcgc gacgggccag gcccgaccac gcgccggggc gccgacgagt tcgtcgcggt
    20221 gtcctgggag gaactcaccg aacttctggc cggcgagctg cggcgcgtgg tcgacatcca
    20281 cgggaacgag gccatctacg gcggctcgta cggctgggcc agcgcaggcc gcttccacca
    20341 cgcccagagc caggtgcacc ggttcctcaa actgcttggc gggtacacct tttcgcggca
    20401 ttcctacagt ctcggcgcca ccggcgtcat catgccgcgg gtggtcggca cccaccacga
    20461 tctcttcaag cggtccaccg cgtgggaggt catcgtcgag cacaccgatc tgctggtgtg
    20521 cttcggtggg ctcgctctga agaacaccgg catcaaccat ggtggcacca ccggccaccc
    20581 cgcccgcgac gccctgcggc ggttccgtga ccgcggagga cacatcgtgt cgttctcgcc
    20641 actgcgtgac gatgtcgagg gtgagtgcga atggcacgcg ccggtgccgg gcaccgacgt
    20701 cgcgatcatg ctggcgctgg cctacgtgct ggccaccgaa tcgctggccg acaaggattt
    20761 cctgaacacg tactgcaccg gttacgaccg cttcgagcgt tacctgctcg gtcttgatga
    20821 cggcgttgcg aagtcgccgc agtgggcggc atcgatatgc ggactgcgcg ccgacgatct
    20881 gatcacgctg gcacgacgga tggcggcgag gcgcaccctc gtgaccgtca gctggtcgct
    20941 gcagcgcatt cggcacggcg agcaggcgcc gtggatgggt ctgacgctgg cggcaatgct
    21001 gggccaaatc ggccttcccg gaggtggttt cggccacggc tacggctcga tgaacgaacc
    21061 cggactgccg ccgctgcgct ttggcctgcc cagactgccg cagggtccca atcccgtgca
    21121 gacattcatt cccgtcgctg caatcagcga catgctgctg caccccggcg aaccgttcga
    21181 ctacaacggc cagcggctga ggtatcccga catcaagttg gtgtactggg ccggcggcaa
    21241 cccatttcac catcatcaga acatcccgcg gctgcgtcgc gcgctcggcc gcgtcgacac
    21301 catcgtcgtg cacgacccgt actggacggc gatggccaag cacgccgata tcgtcgtgcc
    21361 gtccaccacg ccctacgaac gcgacgacta ctccggatcg cgcaacgacc cgctgctgat
    21421 ggccatgccg aagttggcgg agccgtacgc gcaatcccgc gacgactaca cgacgttcgc
    21481 agcgctggcc gaggcgctcg ggttcggcga gcagttcacc gaaggccgca ccgcgcggca
    21541 gtggctcacg cacatgtacg acaaatgggc ggcgggactg gatttcgcgg tgccgacgtt
    21601 cgacgagttc tgggcgatgg gcaggctgcg gctgccgacc gagaacgggc tgacgctgct
    21661 ggccgacttt cgggccgatc cgcagaccca tcggctggcg accgcaagcg ggcgcatcga
    21721 ggtcttctcg gaagacatcg acgggttcgg ctacgacgat tgcgccggtc acccctcgtg
    21781 gttcaagcca tccgagtggc tcggcggccc tcgcgcggcg gactacccac tgcacctgct
    21841 ggcgaaccag ccggcgacgc ggctgcacag ccaactcgat atcggtaaag tgagcatcgc
    21901 ttcgaaagtg caagggcgag agccgatccg gatgcattcg gccgacgcga aatttcgcgg
    21961 gctcgtcgac ggagacgtgg tgcgggtgtt caacgaccgc ggcgcatgcc tggccggcgt
    22021 agtggtcgac gatcgcctgc gccgcgatgt ggtgcaactg tcgaccggcg cctggttcga
    22081 cccggccgac cccgccgacc cggatgccat gtgcgtgcac ggcaatccga atgtgctcac
    22141 cgacgacgca ggcacgtcgt cattggcgcg cggctgtatc ggcgcgcacg tgctggtgca
    22201 ggtggagaag ttcaccgggc cgatgccgcc ggtgcgggcg catgagcccc cgctgatcgt
    22261 cgaccgatga ccgaggcgct ggccgtcgaa gagcgtggcc ggacaatcac attcacgttc
    22321 gacgacatga tgcgttacca cggcgggcac tcccccgccg gtgtggcgat ggcattcaag
    22381 gtcatgcagc gttcgtttgc gatgctctct cccaacgggc cacccgagcg tcgctcgatc
    22441 accgtgcgca ccgcgtttcg gggccccggc gctcgcgacg gctttgaggc ggtgacgcgt
    22501 gcggtgtccg acggccgcta catcgtcgac cgaaccctgg tgaggacgga ccgcggccgg
    22561 cgactggagg acttcgtgtt cgtgatcgag atcgggcggc acaatgtgac cctgtttctg
    22621 cgtgacggat tcgtcaccga cgagtttgtc gatctggcac gcaccgagaa ccgtagcgac
    22681 gcacaggaac aacgactaga cgaactgaaa gcgcaactcg cgcagcgcgt actgggcgct
    22741 caagccgaag aattgttcga cgccgtgtga cggaagtgaa ctgcgacacc gttggtttgc
    22801 cggtggcggt aattggggaa cccccttgcc accatcggaa tgacagggag gaacttgcga
    22861 tgtcggagaa gaacagcggg cccgaggaag ccatcaaggg cgttgtcgag ggggtaaagg
    22921 gcaaggtcaa agaggttgcc ggcgcggtag ccggccgcga cgacctgtac cgcgagggcc
    22981 aagcccagca ggacaaggcc gatgcgcaac gcaacgccgc gcagaaggaa gccgaggccg
    23041 aaagcgctcg cgcggccgcc aagaccgccg aaaagcgtca ggaggccgag cagaggtaag
    23101 aacgtgtcag cgaaggggcg gcgtccgcgg tggcgccgcc cttcgcgcac ccgtcgcctc
    23161 aaatgccgcc gcaaggcgca gcagcgcaac atcgtcatag gcgcggccgg cgatggtgag
    23221 ccctaccggc atccccgtgt cgtccattgt gcccatcggc acggtcaccg tggggatgcc
    23281 gaggtgccgg atcgccaggt tgccgttggc cacccagacg ccgttgcgcc aaccgagatc
    23341 ggccgaggac tcgttgacgt ccatgtccgc cggcccgaca tccgcggccg ccgggaacac
    23401 caccgcgtcc aggccgaggc cgtccatcca ctcgtctaga tcgacgcgcc gcgtctcctc
    23461 gagcccgcgc acgccctcct ccagatgcgg tatgtcgctg aaggattcga tcgggtggtc
    23521 cctgacgtgc gccggataac ttgcgatgtc gtcgtcgaac ccggtgtacc ggtccgggag
    23581 cgctccctct ggatgcggga agatgcgtga gccgtccacg tcggccaacc ggttcagcgc
    23641 ctggtctccg ttgtcgtcga gaaagtcgtc ccatgcccac gcgctgaggt caaggagctc
    23701 tttggccaga tactccgggc tcaccagacc ccgtgtgaaa atcgttggcg cgccgggccg
    23761 gtcgccttcg tagttgctga ccaccggaaa gtcgacatcg atgacctcgg cgccgacggc
    23821 ctccagatcg cgtcgtgcgc gctcgaagag tccgagcact gacggccgcg tctggatgcg
    23881 ctgacccgtt ggcccgccga tccccgccgg ttcggcggtc ccggcgtcgg gatcggcgtt
    23941 gatgtacatg cgcggcacgc cgaaccgccg cccggcgagc acctgacgcg cctcctcacg
    24001 gctcgtcgga cgcaactgcg gatacgatgc cggccgcaga tcactgggtc gaggtatcgc
    24061 aatccacggc tggctgcgcc agaagtctcc gcgcacacgc ggatcgtcgg ccacgatgac
    24121 gtcaagaact tcgagcatgt cggccatcgt cctggcatgt ggcacaacga catccatcgt
    24181 cggcaccaag ggccagccgc ctcgcgtcga gatgacgccg cgcgacggcg tgtatgcgca
    24241 cagcgcattg ttcgacgccg gagcgcgccc ggacgaccac gtctcctcgc caatgccgaa
    24301 ggcgcagaac gacgccgcgg tggccgtgcc tgagccgttc gacgagccgg acccgaacgc
    24361 agcggtgagg tactcggcgt tatatgggct ttctgcgcgc ccatacaatc cgcgctgcat
    24421 gccgccgttg gccatgggcg gcatgttggt cagcccgatc aggatcgcgc cggcggcgcg
    24481 cagccgctcg ataacaaaac agtccttctg ggcgaccagg tcgg
//
LOCUS       DASRJA010000612         9093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1569089,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000612 DASRJA010000000
VERSION     DASRJA010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9093
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569089"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VFC01_27290"
     CDS             complement(<1..146)
                     /locus_tag="VFC01_27290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZN83355.1"
                     /translation="MVARVCVVGSVNADLTFTVDALPRPGQTILASSLSSSPGGKGGN
                     QAVAA"
     gene            223..1470
                     /locus_tag="VFC01_27295"
     CDS             223..1470
                     /locus_tag="VFC01_27295"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HZN83356.1"
                     /translation="MSVQAPAVSGLREQVHDAARRARVAARELATLSTAAKDRALHTA
                     ADCVLARTHDILAANAEDLAAARDAGTPDAMLDRLALNPARVDGIAHGLRQVAGLPDP
                     IGDVLRGRTLPNGLQLRQQRVPLGVVGIVYEGRPNVTVDAFGLTLKSGNAVLLRGSSS
                     AAHSNAALVAALQAALSNEGLTRDAVQLLPSEDRASVTHLIQARGLVDVVIPRGGAGL
                     IDAVVRDAMVPTIETGVGNCHVYVHESADLDVAEHILLNAKTRRPSVCNAAESLLIDR
                     SIAEQAVPRLTRALQDAGVTVHSDPTEEELRAEFLSMDIALAVVDGLDAAIAHVNEYG
                     TGHTEAIVATNLAAAQRFTDRVDAAAVMVNASTAFTDGEQFGFGAEIGISTQKLHARG
                     PMGLPELTSTKWIVWGDGHTRPA"
     gene            1483..2358
                     /locus_tag="VFC01_27300"
     CDS             1483..2358
                     /locus_tag="VFC01_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN83357.1"
                     /translation="MSVPARPAPLFADIDDVARRLAETGYLPDTATATAVFLADRLGK
                     PLLVEGPAGVGKTELARAVAQATGSGLVRLQCYEGVDEARALYEWNHAKQILRIQAGH
                     GDWEQTKTDVFSEEFLLTRPLLTAIRRTEPTVLLIDETDKADIEIEGLLLEVLSDFAV
                     TVPELGTITAERAPFVLLTSNATRELSEALKRRCLFLHIDFPDPDLERRILLSRVPEL
                     PEHLAAELVKIIGVLRGMQLKKLPSVAETIDWGRTVLALGMDTIDDEVIAATLGVVLK
                     HQSDQVKASGELRLN"
     gene            2358..3803
                     /locus_tag="VFC01_27305"
     CDS             2358..3803
                     /locus_tag="VFC01_27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN83358.1"
                     /translation="MAARRTRPPQPLAPHGLPGHLVEFVEALRKQGISVGPSETVDAG
                     RVLSVLGLGDREALREGFACAVLRRSDHRDTYDAMFDLFFPAALGARTVVLDDDEEPD
                     DDLGLPPEDIEALRAVLIDMLSENADLANMDERLAAMIAQIVEAYGRYQSSRGPSYSS
                     YQALKAMSLDDLEGRLLAGLLAPYGDEPTPSQEQIAKALAAQRIAQLRKMVEAETKRR
                     TAEQLGRDHVQMYGVPQLAENVEFLRASGEQLRNMRRVVAPLARTLATRLAARRRRSR
                     AGEIDMRKTLRKSMSTGGVPIDVVLKKPHPARPELVVLCDVSGSVAGFSHFTLMLVHA
                     LRQQFSRVRVFAFIDTTDEVTELFGPDADLAVAVQRITREAGVYTRDGHSDYGNAFVS
                     FMEKWPNVLSPRSSLLVLGDGRNNYRNPETELLAHMVNASRHAHWLNPEPRHLWGSGD
                     SAVPRYQDVITMHECRSAKQLAAVIDQLLPV"
     gene            3931..4554
                     /gene="nadD"
                     /locus_tag="VFC01_27310"
     CDS             3931..4554
                     /gene="nadD"
                     /locus_tag="VFC01_27310"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZN83359.1"
                     /translation="MGVMGGTFDPIHNGHLVAASEVADLFELDEVVFVPTGQPWQKHH
                     RYVTAAEDRYLMTVIATASNPRFSVSRVDIDRAGPTYTKDTLRDLRALNPDTDLYFIT
                     GADALASILSWQNWEEMFSIAKFVGVSRPGYELHGKHIAAALAELPPDALTLVEVPAL
                     SISSTDCRKRAEEDRPIWYLVPDGVVQYVAKRKLYAAQPVVAKDGNR"
     gene            4551..4919
                     /gene="rsfS"
                     /locus_tag="VFC01_27315"
     CDS             4551..4919
                     /gene="rsfS"
                     /locus_tag="VFC01_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZN83360.1"
                     /translation="MTATDEAVKMATVAARASASKLAEDVVVIDVSGQLVITDCFVIA
                     SASNERQVNAIVDEVEEKMRLAGYKPARREGAREGRWTLLDYVDIVVHIQHEDERDFY
                     ALDRLWRDCPVVSVDLDGTP"
     gene            4916..5584
                     /gene="gpgP"
                     /locus_tag="VFC01_27320"
     CDS             4916..5584
                     /gene="gpgP"
                     /locus_tag="VFC01_27320"
                     /EC_number="3.1.3.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate phosphatase"
                     /protein_id="HZN83361.1"
                     /translation="MRVRRLVMLRHGQTGYNAGNRMQGQLDTELTDLGREQAVAAAEV
                     LAKRQPLMIVSSDLRRALDTAVALGERCGQPVLVDTRLRETHLGDWQGLTHLEVDAAA
                     PGARLAWRDNARWAPHGGESRVDVAARSMPLVAELVADQTEWGVDEPERPVVLVAHGG
                     LIAALTAALLALPVDNWPALGGMGNASWVQLSGHAQPDASLDGIRWRLDVWNASAQVA
                     NDVL"
     gene            5574..6314
                     /gene="octT"
                     /locus_tag="VFC01_27325"
     CDS             5574..6314
                     /gene="octT"
                     /locus_tag="VFC01_27325"
                     /EC_number="2.3.1.273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016414 - O-octanoyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosylglycerate octanoyltransferase"
                     /protein_id="HZN83362.1"
                     /translation="MSSDSPVRPTLLVFCDSLSYYGPTGGLPADDPRIWPNIVANQLG
                     WDVEVIGRIGWTCRDVWWAATQDPRAWAALPRAGAVIFATGGMDSLPSPLPTALRELI
                     RYVRPAWLRRWVRDGYGWVQPRLSPVSRAALPPHVSVEYLEMTREAIDFNRPGIPIVA
                     SLPSVHIAQTYGKAHHGREGTAKAITAWAEQRNVPLVDLKAAVGEHVMSGRGNPDGIH
                     WNFESHQAVAELMLKALAEAGVQQEDSR"
     gene            6317..7156
                     /locus_tag="VFC01_27330"
     CDS             6317..7156
                     /locus_tag="VFC01_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZN83363.1"
                     /translation="MAVVVVTDSSARLQPDELKQFDIRQVPLHVLVDGTDLRDGVDDV
                     PYDIHDRPRVTTAGATRAELAETYQQALTDSGGDGVVAVHLSAALSSTYSSAVQAARE
                     FGPAVRVVNTRSVAMGVGFVALATARSAGSGADLDAVEAAARSAQARGHVFVVVHRLD
                     NLRRSGRISKGASRLGTALSLKPLLCLDVDGRLVLDQRIRTITKAHAAMVDRVAEVVG
                     DRRASIAVHHVDNHDAADEIGAALTTRLPQIESLVVTDMGPVLSIHVGGGAVGVAVQL
                     VDY"
     gene            7215..7541
                     /locus_tag="VFC01_27335"
     CDS             7215..7541
                     /locus_tag="VFC01_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN83364.1"
                     /translation="MSTYQEDADAWVALADRTRRSIVERLAHGPLAVGELARDLPVSR
                     PAVSQHLKVLKRAGLVRDRAAGTRRVYQLDPTGLEALRADLDRFWVQALAGFKDTVER
                     LEGDES"
     gene            7538..8014
                     /locus_tag="VFC01_27340"
     CDS             7538..8014
                     /locus_tag="VFC01_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN83365.1"
                     /translation="MTQARPVTVRHDVVVNAPVERAFRVFTERFGDFKPREHNLMSAP
                     IAETVFEPRVGGHIYDRGVDGSLCKWARVVAFEPPNRVVFTWDIGPTWQLETDLTRAS
                     EVEVRFTAESADRTRVELEHRHIERHGEGWESVAAGVDGDAGWPLYLGRYAGLFEQ"
     gene            8011..8631
                     /locus_tag="VFC01_27345"
     CDS             8011..8631
                     /locus_tag="VFC01_27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HZN83366.1"
                     /translation="MTSTQQMARDERVEFASFLEGLTPEQWDNPTLCDKWRVREVAIH
                     TVSYDELNTPGLVRRFLKGRLSTDRINAVGVADYADRTSEQIVAMIRANVEPHGLTGG
                     FGGKIALTDGMIHQQDIRRPLGLLRTIDPERLRTALDFARFAPTIRGAWRARGTRLTA
                     TDLDWAFGKGPEVHGPGEALLMAMAGRGAALDDLDGPGKAKLAQRF"
     gene            complement(8628..>9093)
                     /locus_tag="VFC01_27350"
     CDS             complement(8628..>9093)
                     /locus_tag="VFC01_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN83367.1"
                     /translation="SEVFAESAAMMADAIGAKLDRMTFDVTFTAATGDSDLGFMQIPA
                     GTVGGVMGYHRGWVGEKNVVSVGFNWIMGDHVTPPKPLEHGHVIQVFGLPNMRTVLHC
                     LPPKDWTEPGFMGLGMIYTAMPVTNAVPAVVAAPPGIVTLKDLPPITGRSAV"
BASE COUNT         1527 a         3022 c         3093 g         1451 t
ORIGIN      
        1 gccgccaccg cctgattgcc gcccttgccg cccggcgacg acgacagcga tgacgcgaga
       61 atcgtttggc cgggccgggg cagcgcatcc acggtgaaag tgaggtcggc attgacactg
      121 ccgactacgc agacccgcgc gaccataact gtcaacgcta actcagcttg ggccgacctg
      181 acacccgtcg agcgcctctg gtatgtccga tacgctgggt caatgagtgt ccaagcacca
      241 gccgtctccg gcttgcgcga gcaggtgcac gacgccgcgc gacgggcccg agtcgccgcc
      301 cgtgagttgg ccaccttgag caccgcggcc aaggatcgag cactgcacac cgccgccgac
      361 tgcgtgctgg cacgcaccca cgacatcctg gcagccaacg ccgaagacct ggcggccgcg
      421 cgcgacgccg gcacacccga cgcgatgctc gaccggctcg cgctcaatcc ggcgcgcgtc
      481 gacggcatcg cccacgggct gcggcaggtt gccggcctac ccgaccccat cggcgatgtg
      541 ttgcgcggcc gcaccctgcc caacgggctc cagctgcgac agcagcgggt tccgctcggc
      601 gtggtgggca tcgtctatga gggccggccc aacgtgacag tcgacgcgtt cggcttgacg
      661 ctcaagtccg gcaatgcggt gctgctgcgc ggcagttcgt cggctgcaca ttccaacgcg
      721 gcattggtcg ccgcgctgca ggcagcgttg agcaacgagg gccttacgcg agacgccgtg
      781 caactgttgc caagcgagga ccgcgccagc gtcacgcatc tgattcaggc ccgcgggctc
      841 gtcgacgtcg tgattccgcg cgggggagcg ggtttgattg acgccgtggt gcgcgatgcg
      901 atggtgccca ccattgagac cggcgtcggc aattgtcatg tgtacgttca tgaatcggcc
      961 gacctcgacg tggccgagca catcctgctg aacgccaaga cacgcaggcc gagtgtctgc
     1021 aacgccgccg agtcgctgct gatcgaccgg tcgatcgccg agcaggcggt gccgcggctg
     1081 accagggcgc tgcaggacgc tggtgtcacg gtgcatagcg atccgaccga ggaggaactg
     1141 cgcgccgaat tcctgtcgat ggatatcgcg ctggcggtgg tcgacggcct cgacgcggcg
     1201 atcgctcacg tcaacgagta cggcacgggt cacaccgagg ccatcgtcgc aacgaacctt
     1261 gctgccgccc aacggttcac cgaccgggtc gacgcggccg cggtaatggt caacgcgtcg
     1321 acggcgttca ccgatggtga gcagttcggg tttggcgccg agatcgggat ttccacccag
     1381 aagctgcacg cccgcgggcc gatgggcctg cccgaattga cgtcaaccaa gtggattgtg
     1441 tggggagacg gccacacccg cccggcctaa caggagaatt aagtgagcgt ccccgcacgc
     1501 ccggcgccgc tgttcgccga catcgacgat gtcgcgcgac ggctggccga aaccggctac
     1561 ctgcccgaca ccgcaacggc cacagcggta ttcctcgccg accgactggg caaaccgttg
     1621 ttagtggaag gccccgcagg cgtcggcaag accgaactgg cacgtgcggt cgcgcaggcc
     1681 accggttccg gcctggtgcg gctgcagtgc tacgagggcg tcgacgaggc ccgtgcgctc
     1741 tacgagtgga accacgctaa gcagatcctg cggatccagg ccgggcacgg cgactgggag
     1801 cagacgaaaa ccgatgtgtt cagtgaggaa ttcctgctga cgcgaccgct gctgaccgca
     1861 atccggcgca ccgagcccac cgtgctgctg atcgacgaga cggacaaggc cgacatcgaa
     1921 atcgagggcc tgctgctgga ggtgctgtcc gacttcgcgg tgaccgtgcc ggagctcggc
     1981 acgatcaccg ccgagcgcgc gccgttcgtc ctgctgacct ccaacgccac ccgagagtta
     2041 tccgaggctt tgaagcgacg ctgtctgttc ctgcacatcg actttcccga tcccgacctc
     2101 gagcgccgca tcctgctgtc ccgtgttccg gagttgccgg agcatctggc tgccgaactc
     2161 gtcaagatca tcggggtgct gcgcggcatg cagctgaaga agttgccgtc ggtggccgag
     2221 accatcgact ggggccgcac ggtgttggcg ctcggcatgg acactatcga cgacgaggtg
     2281 attgccgcca ctctcggcgt ggtgctcaag catcagtccg atcaagttaa agcttccggg
     2341 gagttaaggc tgaactgatg gccgcccgcc gcacccgccc gccacagccg ctggcgccac
     2401 atggacttcc gggtcacctg gtcgagttcg tggaagccct acgtaagcag gggatttcgg
     2461 tcggaccatc ggagacggtt gacgccggtc gggtgctgtc cgtgttgggg ctgggcgacc
     2521 gtgaggcact gcgcgagggc ttcgcatgcg cggtgttgcg acgctccgac caccgtgaca
     2581 cctacgacgc gatgttcgat ctgttcttcc cggcggcact gggcgccagg acggtggtgc
     2641 tcgacgacga cgaagaaccc gacgacgatc ttggcttacc gcctgaggac atcgaggcgc
     2701 tgcgcgccgt actcatcgac atgctcagcg agaacgccga tctggccaac atggacgagc
     2761 gactcgcggc gatgatcgcc cagatcgtcg aggcctacgg ccgctaccaa tccagccgcg
     2821 gcccgtcgta ttcgtcgtat caggcgctca aggccatgag cctggacgat ctggagggca
     2881 ggctgctggc cggtctgctg gcgccgtacg gcgacgagcc gactccgtcg caggagcaga
     2941 tcgccaaagc gcttgccgcc caacgcatcg cgcagctgcg caagatggtg gaggcggaga
     3001 ccaagcgccg caccgccgag cagctgggtc gggatcacgt gcagatgtac ggcgtcccgc
     3061 agcttgccga gaacgtcgaa ttcctgcgcg cctcaggcga acagctgcgc aatatgcgac
     3121 gggtggtggc gccgctggcg cgaacgctcg cgacgcggct ggccgcgcga cggcgacgat
     3181 cgcgggccgg tgagatcgac atgcgcaaga cactgcgtaa gtcgatgtcg accggcggcg
     3241 tgccgatcga cgtcgtgctg aagaagccgc atcctgcgcg accggagctg gtggtgttgt
     3301 gcgacgtgtc cggctcggtg gcgggcttca gccacttcac gctgatgctg gtgcacgcgc
     3361 tgcgccagca gttctcccgg gtgcgcgtgt tcgcgttcat cgataccacc gacgaggtca
     3421 ccgagctgtt cgggcccgac gccgacctgg cggtggcggt gcagcgcatc acccgcgaag
     3481 ccggggtgta cacccgcgac ggccactccg actacggcaa cgcgttcgtc tccttcatgg
     3541 agaagtggcc gaacgtgctg tcgccgcgca gctcgctgct tgtgctcggc gacggccgca
     3601 acaactaccg caacccggaa accgagttgc tggcccacat ggtcaacgcc agcaggcatg
     3661 cgcactggct caatcccgag cccaggcatt tgtggggcag cggcgattcg gcggtgccgc
     3721 gctatcagga cgtcatcacc atgcacgaat gccggtcggc caagcagctg gccgcggtga
     3781 tcgaccagct gctgccggtc tagatttcgg cgaacgtggg ttaccgtcac gctttcgcga
     3841 ggggaagcgt gacggtaacc cacgttcggc gcgggaagag tcgccgcaag cactcccgta
     3901 agctggctgt ttgtgacttc tcgacggcgg ctgggggtga tgggcgggac gttcgatccc
     3961 atccacaacg gccacctggt tgctgccagc gaggtcgccg acctgttcga gctcgacgaa
     4021 gtggtgttcg tgccgactgg ccagccctgg caaaagcacc accgctacgt caccgccgcc
     4081 gaggaccgct acctgatgac cgtcatcgcg accgcgtcga accctcgatt ctcggtcagc
     4141 cgggtggaca tcgaccgcgc cggcccgacc tacaccaagg acacgctgcg cgatctgcgg
     4201 gcgcttaacc ccgacaccga tctctacttc atcaccggcg ccgacgcgct ggcctcgatc
     4261 ctgtcctggc agaactggga ggaaatgttc tcgatcgcca agttcgtcgg cgtcagcagg
     4321 ccgggctacg aactccacgg caagcacatc gctgcggcgc tcgccgagct gccacccgac
     4381 gcgctgaccc tggtcgaggt acccgcgctg tcgatctcgt cgaccgactg ccgcaagcgc
     4441 gctgaggagg accggccgat ttggtacctg gtgcccgacg gtgtcgtgca atacgtggcc
     4501 aagcgcaagc tctacgccgc ccagcccgtc gtggccaagg acggcaaccg gtgacggcca
     4561 ccgatgaagc ggtgaagatg gccacggtgg cggcgcgcgc gtcggcgtcc aaactcgccg
     4621 aagacgtcgt cgtgatcgac gtgtccggcc agcttgtgat caccgactgc ttcgtcatcg
     4681 cgtcggcgtc caacgagcgc caagtcaacg cgatcgtcga cgaggtcgag gagaagatgc
     4741 gcctcgcggg ctacaagccc gcccgtcgcg aaggcgcccg cgagggccgt tggacgttgc
     4801 tcgactatgt cgacatcgtg gtgcacatcc agcacgagga cgagcgtgac ttctacgccc
     4861 tcgaccggtt gtggcgcgac tgccccgtcg tgtcggtcga tctggacggg acgccatgag
     4921 ggtgcgccgc ctggtcatgc tgcgtcacgg gcagacggga tacaacgccg gcaaccggat
     4981 gcagggccag ctcgacacgg agctgaccga cctgggccgc gagcaggccg tcgccgccgc
     5041 ggaggtgctg gccaaacggc aaccgctgat gatcgtgtcg tcagatctgc ggcgcgcgct
     5101 ggacaccgcg gtcgcgctcg gtgagcggtg cggtcagccc gtgctcgtcg acacccggct
     5161 gcgtgaaacg cacctcggcg actggcaggg attgacccat ctcgaagtgg acgccgccgc
     5221 cccgggtgcg cggctggcgt ggcgggacaa cgcccggtgg gcgccgcacg gtggcgaaag
     5281 ccgggtcgac gtcgccgcac gcagcatgcc gctggtggcc gaactggtcg ccgaccaaac
     5341 cgagtggggc gtcgacgaac ccgaacggcc ggtggtgctg gtggcacacg gcgggctgat
     5401 cgccgcactg accgcggccc tgctcgcgtt gccggtcgac aactggcccg cgctgggcgg
     5461 catgggcaac gccagctggg tgcagctgtc gggtcacgcg cagcccgacg cctcgctgga
     5521 cggtatccgc tggcgcctcg acgtgtggaa cgcctcggca caggtcgcca acgatgtcct
     5581 ctgattcgcc ggttcggcca acgcttctcg tcttctgcga ctcgctgtcc tactacgggc
     5641 ccaccggcgg gttgcccgcg gacgatccgc ggatctggcc gaatatcgtt gccaaccaac
     5701 tgggttggga tgtcgaagtg atcggacgga tcggctggac ttgcagagac gtctggtggg
     5761 ccgccacgca ggacccccgg gcgtgggcgg cgctaccccg cgccggcgcc gtgatcttcg
     5821 cgacgggcgg catggattcg ctgccgtcgc cgctgcccac cgctctgcgg gagctgatcc
     5881 gctacgtccg gccggcgtgg cttcgacgct gggtgcgcga cggatacggc tgggtgcagc
     5941 cgcggctgtc gccggtatcg cgcgccgcgt tgccgccgca tgtttctgtt gagtacctcg
     6001 aaatgacccg tgaggcaatc gacttcaata ggcccggaat accgatcgtc gcatcgctgc
     6061 cgtcggtcca catcgctcag acctacggca aggcacacca cggccgtgaa ggcaccgcca
     6121 aggcgatcac cgcgtgggcc gagcagcgca acgtcccact cgtcgacctg aaggcggccg
     6181 tcggcgaaca cgtgatgagc gggcgcggca accccgacgg tatccactgg aacttcgagt
     6241 cacaccaggc agttgccgag ctgatgctca aggcgctcgc ggaagcgggt gtgcagcaag
     6301 aggattcgcg atagtcatgg ctgttgtcgt ggtgaccgat tcgtcggcgc gcttgcagcc
     6361 agacgagctc aagcagttcg acatccggca agtgccgctt cacgttctgg tcgacgggac
     6421 cgatctgcgt gacggggtcg acgacgtgcc gtacgacatc cacgatcggc cgcgggtcac
     6481 caccgccggc gccacacgtg cggagctggc cgagacgtac cagcaggcgc tgaccgacag
     6541 cggcggcgac ggcgttgtgg cggtacatct ttcggcggcg ctgtcgagca cctacagctc
     6601 ggcggtgcag gctgctcgtg agttcggccc ggcggtacgg gtggtcaaca ctcgttcggt
     6661 ggcgatgggg gtcgggttcg tcgcgctggc cacggcgcgt tcagcaggat cgggggccga
     6721 tctcgacgcc gtcgaggccg cggcccggtc ggcgcaggcg cgcgggcacg tgttcgtcgt
     6781 cgtgcaccgg ctggacaatc tgcggcgcag cggccggatc agcaaggggg cgtcgcggct
     6841 gggcaccgcc ttgtcactga agccgctgct gtgcctcgac gtcgacgggc gcctggtgct
     6901 ggatcagcgg atccgcacga tcaccaaggc gcacgccgcg atggtcgatc gcgtcgccga
     6961 agtggtcggg gaccgcaggg catcgatagc ggtgcaccac gtcgacaatc acgacgccgc
     7021 cgacgagatc ggcgccgcgt tgacgacgcg attgccgcag atcgagtcgc tcgtcgtcac
     7081 cgatatgggg ccagtgttgt cgatccatgt cggcggcggg gcggtcggcg tcgccgtgca
     7141 actggtcgac tactaacgct gcgtccaagc atcgcccatt gatgcccgca ttggtaagtg
     7201 gtaacttacg gttcgtgtcc acttaccaag aggatgccga cgcctgggtg gcgctggcgg
     7261 atcgcacccg gcggtcgatt gtggaacgcc tcgcgcacgg gcctttggca gtgggcgaac
     7321 tggcccgaga cctgcctgtc agcaggcccg cggtgtcgca acacctcaag gtcctcaagc
     7381 gtgccgggct ggtgcgggac cgtgccgcag gcacacgacg ggtctaccag ttggacccga
     7441 cagggctgga agcgttgcgc gccgacctcg accggttctg ggtgcaggcc ctggccggtt
     7501 tcaaggacac cgtcgagcgt ctggaaggag acgaatcatg acgcaggccc gacccgtaac
     7561 cgtccgtcac gacgttgtcg tcaacgcccc tgtcgagcgg gcgttccgcg tgttcaccga
     7621 gaggttcggc gacttcaagc cgcgcgaaca caatctgatg agcgccccca tcgccgagac
     7681 cgtgttcgag cctcgcgtcg gcggacacat ctacgaccgc ggcgtcgacg gcagcctgtg
     7741 caagtgggcg cgggtcgtcg ccttcgagcc gccgaaccgc gtcgtcttca cgtgggacat
     7801 cggaccgacc tggcagctgg aaaccgacct cacccgggca agtgaggtcg aagtccgctt
     7861 cacggccgag tccgccgacc ggacgcgcgt cgaactggaa caccgccaca tcgaacgcca
     7921 cggtgagggc tgggagtccg tcgcagccgg agtcgacggc gatgccggct ggccgctgta
     7981 cctcggccgc tacgccggcc tgttcgagca atgacgtcaa cccagcagat ggctcgcgac
     8041 gagcgcgtcg aattcgcctc attcctagag ggtttgaccc cagagcaatg ggacaacccg
     8101 acactgtgcg ataagtggcg agtccgcgaa gtcgcgatcc acacggtgag ctacgacgag
     8161 ctcaacaccc ctggtctggt ccgtagattc ctgaaaggtc gactgagcac ggaccgcatc
     8221 aacgcggtcg gcgttgccga ttatgccgac agaacgtccg agcagatcgt cgcgatgatc
     8281 cgggcgaacg tcgaaccaca cggtctgaca gggggtttcg ggggcaagat cgcgctcacg
     8341 gacggcatga tccatcagca ggacatccgt cgaccgctcg gtctgctgcg caccatcgat
     8401 ccggagcggc tgcgcaccgc gcttgatttc gcccgcttcg ccccgaccat tcgcggcgcg
     8461 tggcgggctc gcgggacccg cctgacggcc accgacctcg actgggcgtt cggcaagggg
     8521 cccgaagttc acggcccagg cgaagcgctg ctgatggcga tggcaggccg cggcgccgcg
     8581 ctcgacgacc tcgatggccc gggcaaagcc aaactggccc aacgcttcta gacggcggag
     8641 cggccggtga tcggcggcag gtccttcagc gtcacgatcc cgggcggggc ggcgacgacc
     8701 gcagggaccg cgttcgtcac cggcatcgcg gtgtagatca tcccgaggcc catgaagccg
     8761 ggctccgtcc aatccttcgg cggcaggcag tgcagcaccg tgcgcatgtt cggcaggccg
     8821 aagacctgaa tgacgtgacc gtgttcgagc ggcttgggcg gtgtcacgtg atcgcccatg
     8881 atccagttga atccgacgct gacgacgttc ttttcgccga cccacccgcg gtggtagccc
     8941 atgacaccgc ccacggtgcc cgccgggatc tgcatgaaac cgagatcgga atcgccggtg
     9001 gccgcggtga atgtgacgtc gaaggtcatc cggtccagct tcgcgccgat cgcgtcggcc
     9061 atcatcgcgg cggactcggc gaagacctcg ctt
//
LOCUS       DASRJA010000613        47542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1828235,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000613 DASRJA010000000
VERSION     DASRJA010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 47542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47542
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828235"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFC01_27355"
     CDS             complement(<1..588)
                     /locus_tag="VFC01_27355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN83368.1"
                     /translation="MVPEAPVIEALTLTRTYRGGGHEVRGVDSVSLAAARGEVIAVTG
                     PSGSGKSTLLYLLGGLDTPNEGHVRVTGVDWATLRGRARARFRRRTCGFIVQGLALLP
                     QATAAENVEVPLLLDGIEPTVRDRRVASALERVGLAQDSVKLTDQLSGGQQQRVAIAR
                     ALVAEPAVVLADEPTGSLDSVTAQDIVALLVAAARE"
     gene            complement(578..1270)
                     /locus_tag="VFC01_27360"
     CDS             complement(578..1270)
                     /locus_tag="VFC01_27360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN83369.1"
                     /translation="MTDRTGPAMEARGLYHIYRETEVETIALRGAELTLEPGTWTSVM
                     GPSGSGKSTLVYVLAGLVEPSGGAVVVDGEDLTRLPPPARARWRRGRVGVVLQRDNLH
                     PLLDVAENIELPLRLEGRGRTQIRARVDQLLDEVGLADRRRHRAGQLSGGEGQRAAIA
                     VALAPHPQVLLADEPTGELDESTAAGVLDLLATLKAGEGASILTVTHNPQVAERADRR
                     LSMRDGEILDGA"
     gene            complement(1267..2058)
                     /locus_tag="VFC01_27365"
     CDS             complement(1267..2058)
                     /locus_tag="VFC01_27365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83370.1"
                     /translation="MRRLIIAFMAAVAMAGCGSNGSTSAPPGQPGSINTGSPTAAPSP
                     PDWTVPLMQTPYSPKVDPAQFTDKITNKYFPLVPGTVMVYEGKRDGVPLRIELTVTNE
                     TKDIFGVKTSVVRDIVTGALEERTSDWYAQDPAGNVWYFGEDTKEYTNGVVSSTAGSW
                     EAGVDGALPGIIMQARPVQGDAYRQEYRPTVAEDVALIKQLGASAEVPAGRYSDVLVT
                     NDRDLLDLNKDEDKYFAPGVGLVKLGGLVNGHREDVWPVSILAPK"
     gene            2178..2444
                     /locus_tag="VFC01_27370"
     CDS             2178..2444
                     /locus_tag="VFC01_27370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83371.1"
                     /translation="MDIALNDAAREVPDGPHFAVIAANADGCPQSSVIFVKRTESDRP
                     EGHGMCGYHAGDRAPERRRPSGVHVSDRGGLIHLMKSTVRYQGR"
     gene            complement(2537..2761)
                     /locus_tag="VFC01_27375"
     CDS             complement(2537..2761)
                     /locus_tag="VFC01_27375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83372.1"
                     /translation="MSAEQQQALDSFYALDNVITVKITMPQADWDAVRTEEPQGGRCN
                     FEFTGGARYTWRKATSVEISGTRFLSKMLS"
     gene            complement(3303..3782)
                     /locus_tag="VFC01_27380"
     CDS             complement(3303..3782)
                     /locus_tag="VFC01_27380"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN83373.1"
                     /translation="MMVTPAPSTQQEGSSKCPGISEGTSLADTAGTGAGELGDDERAA
                     LVRGVLARIGDKWSVIILCQLGIEPRRFNELRRLSGGITQRMLSSTLRNLERDGIVSR
                     TVHPSVPPRVEYSLTSVGRSLLTIVQSLAAWTNDNLDFITTARQSYDARTNVESSDP"
     gene            3842..4837
                     /locus_tag="VFC01_27385"
     CDS             3842..4837
                     /locus_tag="VFC01_27385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZN83374.1"
                     /translation="MKAVAVLDYGGPDVLRVVDVPDPRAGSGQIRLRVHAAAVNPADT
                     LLRVGDIDAALAGNLDPPYRPGMDAAGIVHEIGPDTTTDLQVGDRAMAMVVPIDASGG
                     AYAEYVVLQADQVAPAPQGTSHVEAATLPMNGLTALLAVDTLDLDPGAWLAVTGAVGA
                     VGGYVVELAKARGIRVVADASPADEALVRKLGADLIVARGPGFGDRVHEALPDGAAAV
                     ADTALLGEDVSAALRPGGQIAVFRRPGERGTRPYGAPTDVSLRDIWVPEYRRARHKLD
                     YLRGLVEQGKITLRVADVYPAADAPAAHRRLEAGGVRGRLVLDIWAPQEMQEPSQ"
     gene            4834..5952
                     /locus_tag="VFC01_27390"
     CDS             4834..5952
                     /locus_tag="VFC01_27390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN83375.1"
                     /translation="MSGAVDIGVGIIGASPGRSWAAMSHIPALQALSGYRLRAVATGR
                     AASAERAGTEFGVDGFDDPRALIEHPLVDLVVVAVKVTHHQQLVSQALAAGKMVFCEW
                     PLGVDAYEAQSITDEAVRAGVPTVVGLQSRFTAGVRQAAELVRSGHLGRVVSTSIVGS
                     GLAWRRVTDPSHAYMYDGRSGATTLTVPAAHALDALSFVLGETIRYTAAFGLSSPYVE
                     VAGEAEALTVTAPDQIALTAELASGAVASVFYRGGSSRVANLRWEINGTDGDLLLTSP
                     SGNGNIQIGDLALAAGHCTDTKIVEIELPSPTAEVVNVPPGPAVAVAELYAAFARDLR
                     NGTQDAPTFVTALQLHRALAGIERGARWTSPVEVRSTP"
     gene            6113..6853
                     /locus_tag="VFC01_27395"
     CDS             6113..6853
                     /locus_tag="VFC01_27395"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019926345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZN83376.1"
                     /translation="MGQLEGKVALVTGGSAGIGLETARRFAAEGAQVYLTGRRKDVVD
                     AAVSDIDGDVVGVVGDVADLTALDALYATIAERSGRLDVLVANAGGGQVAGLADITEE
                     HYDTTFDANVKGLVFTVQKALPLLQDGASIVLLSSVAGIKGLAGVSVYAATKAAIRNF
                     ARSWAVELAPRGIRVTALCPGPVDTPGLAGFAAQVPSDDAASTIAIPMGRVGDPKEIA
                     AGALFLASNESSFLTGSDLMVDGGQAAA"
     gene            complement(7476..9530)
                     /locus_tag="VFC01_27400"
     CDS             complement(7476..9530)
                     /locus_tag="VFC01_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN83377.1"
                     /translation="MTLDHSCLEIWGILQAPDWYPRFFRGLGSCEQVSGDAQEFEVRL
                     STPRGAVIAHEMRQTLRRSSMLMWFHATQVSHCFVSIRLTPEEGGTRIAVRIFGVGLL
                     HPDLAKASDGAVRNWVREGLLRISDYLEGKQSSLLVNMGDGHSLQLSVLKTMIVSGVV
                     RASRPDRGLRQLNSLAKWGFTLAGGLGAAAARSPHNVASVDRYGTTTYADMAERTACI
                     ASGLVAGGFTGDSKFAVLARNHSAMVECMVAASKLGADLVLLNTGLAARAIDEIVKRN
                     PVDAIFVDDDLEPQVRYLPAEVPRISTHPNSIVPQRRSIDDLISASAGAEPVAPPKRP
                     GKLIVLTSGTTGTPKGARRPTPPGFGAIAAMLSRMPLRRDEVMLLSAPLFHSWGLAAL
                     QLSAPLLATVVLMERFDAEECLKTIALQRCTVLILVPVMLQRMLELPAAVVDRYDTSS
                     LRVVASSGSPISGASVVKFMDTFGDILYNFYGSTEVSWATIADPTDLRVAPTTAGRPP
                     LGTRIAILGQDGNPLPVGAVGRIFVGNDMLFDGYTNAASPAIEDQLMDTGDLGYLDVS
                     GRLFVAGRDDEMIISGGENVFPRPVEEAIAVLPQVADVAVVGVPDPEFGQRLAAFVVR
                     ADGASLDEEMVKDYVRNRLSRFSIPRDVTFVNQLPRTATGKILKRHLTDGQFPLETGW
                     PG"
     gene            9888..11024
                     /locus_tag="VFC01_27405"
     CDS             9888..11024
                     /locus_tag="VFC01_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-beta-xylanase"
                     /protein_id="HZN83378.1"
                     /translation="MHRRTALKIPLYLAAAAALTKLPLVSAETGRWSADRANAWYANQ
                     GWLLGANYVTSNAVNQLEMFQAGTFDPRRIAGELSVAKQIGMNTMRVFLHDQLWAADR
                     AGFSDRLSQFVAIAASHNIKPLFVLFDSCWDPLPRAGRQRAPIAGVHNSGWVQSPGAY
                     RLQDPAYTRVLQSYVTGVVGLFRNDPRVLGWDVWNEPDNPSRDYRKVEHEDKQQLVAA
                     FLPHVFQWVRAVNPIQPLTSGVWQGHWKDPGSRSAICSLQLEHSDVISFHNYGDPAEF
                     EARIDELTPLGRPILCTEYLARRLGSTVEGILPVAKRRNVGAYNWGFVAGRTQTYLPW
                     DSWKRPYTSIPETWFSDLIHPDGRPHDDNEIRFIRQLAGAGRPT"
     gene            11408..12502
                     /locus_tag="VFC01_27410"
     CDS             11408..12502
                     /locus_tag="VFC01_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005072597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HZN83379.1"
                     /translation="MSSHTTSTIFIIGGTGAQGMPIVAALVADKKYSVRVLTRDASSP
                     RAQALLALGNVSMLEGSFADEAILRKGFRGCDGAYVNIDGFNTGEKTETYWAIRSYEI
                     AIEEGIKFFVYGNLDYALKKSGYDSKFRSGHYDGKGRMGEWILFQNQDNSDRMGAAVF
                     TSGPYIEMVISPLTAMTPTIEDGVVTWRVPLGQGAIPHVALEDCGYYARWIFDHPERS
                     NGMNLEVAIAHMTYTAIAEAFQKVTGHPAQYIDTDLDAYWNGPLKGLADLPAGYNADP
                     NDKSTMSFRDNFTGFWNVWKYGKVSRNYDLLDEIHPNRIRSAEQWFRREDQLGRELGK
                     GTLWDRVQPENQTIDSAILKGSADLRTGTL"
     gene            12787..13233
                     /locus_tag="VFC01_27415"
     CDS             12787..13233
                     /locus_tag="VFC01_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HZN83380.1"
                     /translation="MQRVSISRRLGQRWMLLVAVIVVALAGFAVYRLHGIFASHDVTS
                     TPSGSDNDIVPFNPKHVVLEVFGPPGTVATITYLDVNAQPQRVDAATLPWAYDTNTTQ
                     PAVFVNVSAQGDRDSIGCRIKIDDVVKDERSVNTLNPFTYCLDKSG"
     gene            13230..16115
                     /locus_tag="VFC01_27420"
     CDS             13230..16115
                     /locus_tag="VFC01_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN83381.1"
                     /translation="MSTQRVPDFIRRYSVLIALFWLGLAVLTNVFVPQLETVAESHNV
                     SLSPQDSPSLQAAKRIGKVFNEFDSDSSAMIVLEGDQPLGADAHHYYDGLIQKLRQDT
                     KHVQHIQDFWGDPLTAAGSQSSDGKAALVQLYLAGNQGESLANESVDSVRDIVNHTPP
                     PPGIKAYVTGAAPLVTDQFEVGRHGTLKTTLITIGVIALMLFSLYRRLTTVFLVIFTV
                     MIELTASRGVVAVLANAGIIQLSTYSTNLLTLLVIAAGTDYAIFILGRFHEERYTGQD
                     RVSAFNTMYHGTAHIILGSGLTIAGAVLCLTFTRLPYFNSLGVPAGIGVSVAVVAALT
                     LAPALLVIGRHFGLFEPSRPMSTRGWRRIGTAIVRWPVPILLATVAIAAIGLLSLPGY
                     KTSYDARPFMPASAPANVGYAAAERHFSEARLNPELLMIEADHDLRNSTDMILLERVA
                     KAVFHTDGIAQVQSITRPLGTPLDHTSIPFQISAGSASQINNLPFQQARGADLLKQVD
                     VINNSIDVLRQQYALQQQSSAATDEQTKAFQQTVATAQDLRDKIANFDDFFRPLRNYF
                     YWEPHCFDIPICWALRSLFDALDGIDALTVQLSNVSGSIAKLDALQPKLLALIPPQIE
                     SQQTNRDLTLTNYATTSGIDDQTEAALQNATALGQAYDASKTDDSFYLPPEAFANPEF
                     LRGLKLFLSPDGKAARMIITHEGIPATPEGISHIDPIRHAAQEAVKGTPLAGSKIYIA
                     GTAATYKDIQDGAKYDLMIAGIAALCLILLVMMFITRSIVAAIVIVGTVALSLGASFG
                     LSILIWEDLFGIPLFWIVLALGIILLLAVGSDYNLLLISRFKEEIHAGINTGIIRAMA
                     GSGAVVTAAGLVFAATMASFIFADLRILGQIGTTIALGLLFDTLIVRSFMTPSIAALL
                     GRWFWWPLKVRQRPVPQAFALPDQRERQLPLWDDGDSAPTSELPRRG"
     gene            complement(16112..16582)
                     /locus_tag="VFC01_27425"
     CDS             complement(16112..16582)
                     /locus_tag="VFC01_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014818382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83382.1"
                     /translation="MNLGPWGDLTILAAVMEIVFATCVFTYIGRLEKRTSHPLGEQVG
                     AHKAVLAKVRKREPMSQDEVDYARELVSDARSPLAYAIPAALFTIGFFYVVGCLYMLH
                     RDGGNPSFRTFIGGIPMLTSMNIVAQLRRVAGLKGKLRDVSVTAPKPDDELATA"
     gene            complement(16981..17934)
                     /locus_tag="VFC01_27430"
     CDS             complement(16981..17934)
                     /locus_tag="VFC01_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HZN83383.1"
                     /translation="MTETTPNKTASAQNWPSLRVDEWTASRDTLHMWTQIVGKIRLAH
                     APMLNHWWQVTLYVSPRGLTTSAIPYESGIFDIEFDFIEHRLHIRSSDGGARRIALEP
                     RSVADFYAETISALDERGIAVQIQARPNEVDPAIPFAEDHTHASYDPHAVHLFWRQLV
                     SADRVMREFRSHFIGKVSPVHFFWGAMDLACTRFSGRAAPKHPGGAPNCGDWVMVEGY
                     SHELSSCGFWPGGGDEGAFYAYAYPEPDGFADYPVVPEQAFYSTDNGQFLLPYEAVRT
                     AADPDRTLLEFLHTTYRAAAERGRWDRSALEVDPHRWNEKR"
     gene            complement(18137..19051)
                     /locus_tag="VFC01_27435"
     CDS             complement(18137..19051)
                     /locus_tag="VFC01_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN83384.1"
                     /translation="MSVDNISHLGGEGLDELVPSRYALHVGEIEVLVISDGVLPITGK
                     TIAYNADSADLASWLDDMFLPPDVLEWPLNVCVVRSGDRTILIDAGLGGEFPDFPTAG
                     RLALRLEAAGIDSASVTDVVLTHMHMDHIGGLLGDGLGSRLRPDLRVHVAAAEAEFWE
                     SPDFTHTNMPEGVPDALRSAATRFLDEHRGRLRPFETEYEVAPGVLASRTGGHTPGHS
                     VVRLASGGERLTFAGDAVFQVAFEHPDWFNGFEHDPEEAARVRVKLLQELASTREALV
                     ATHLPFPSVCHVAVAGNAFRGVPAVWDY"
     gene            complement(19129..19932)
                     /locus_tag="VFC01_27440"
     CDS             complement(19129..19932)
                     /locus_tag="VFC01_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014928626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83385.1"
                     /translation="MNSPIVLIHGLWLTPRSWEGWKARFEKRGHEVLAPAWPRMEGEV
                     EALRRDPSVMNGLGITEVVDHYDRIIRGLDTPPVIMGHSFGGLITELLLDRGLGAAAV
                     AVSPAQIKGVLRLPPAQIRTVWPVLRNPANRMKTVPLSPKQFHFRFTNTMNDADAAAA
                     YQRYYVPGPGRVLFQAGFANFNPHAVTKVDLHKDDRPPLLVLGNERDHTVPASVSREA
                     AKLLGKSKAVVDYKEFAGRPHFTAGAPGWEAVADYALDWANRHVGVPRA"
     gene            20360..20827
                     /locus_tag="VFC01_27445"
     CDS             20360..20827
                     /locus_tag="VFC01_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83386.1"
                     /translation="MLGPDAVRYRNHVYRGLNYQRKLLQLNTIPDEIAFAWALHDIGV
                     WTSGWDYIRPSLDQVDELAPHNRIAHVDRVRQMVEWHHKVRRCRDDWVVETFRVADRI
                     DVTRGLLRSALTQSDIRQVVNAFPYNGFHALLVRSEAGWAAKHPLRPFPMLRW"
     gene            21177..21848
                     /locus_tag="VFC01_27450"
     CDS             21177..21848
                     /locus_tag="VFC01_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN83387.1"
                     /translation="MTDRISIVIAEDSLLVRDSVSRALSTDPDVTVVGVAEDYYSAVE
                     LVEEHRPTILVTDVRMPPTSTDEGIRLARWTRTAHPDVGVIVLSNYADPSYAAGLLEG
                     GTTGRGYLLKERVARFDELSDAVHQVANGGTVLDPLVVEALLAQPRPAATLNRLTPRE
                     REVLGELATGFSNRTVARRLVLSQRAVEKHINSIFAKLELTPDQSVDRRVKAVLMFLD
                     DSGSV"
     gene            21853..22296
                     /locus_tag="VFC01_27455"
     CDS             21853..22296
                     /locus_tag="VFC01_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN83388.1"
                     /translation="MRPAIGNGGAYTIVFGKSIRDDGGVADLVRVWVVDDQASFRRAT
                     AATLDAMDDFVMAGECETGETAIEQIPNGDADIVLMDIHMPGMGGIEAARHISAARAD
                     VMIVLMSTYDVEDLPVSASVCGAASYLHKERLSPDLLRRIWRAGA"
     gene            22278..23585
                     /locus_tag="VFC01_27460"
     CDS             22278..23585
                     /locus_tag="VFC01_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZN83389.1"
                     /translation="MASGRLRLTSAVGSAPQLRAPRTLAVAALRSPSLGHDLMHRFRA
                     ERFELFLSGHLALWASAGILLPLWEYFWLLRSPRFLAFVAVGAAQCVVLAVGLRLARR
                     DRYQPSITLVCIGTWVSVSLVTLISPPLLPAMVLVAMVPVTFAEPYISLQRGLVFTVI
                     TAGCVLAMAALARITHLPAAVVQETRWIETAFIVIAVPLNVLHVMVIVWNNASALRTS
                     ERKLAEHAAELAASRTRLITAADEERRRLERDLHDGAQQHLVALSVLVQLARNAERDR
                     AAPLLTEASELVDTAISEIRRLAHGIYPPLLISGGLAEALPALAARASVPVQLDLGRI
                     DRYPPSTEAAFYYCCSEALQNAAKHGGPDTTVTVTAVADEQTLTLTISDTGCGFTPAI
                     MGMGLTNMTDRLSAIGGRLGIDTAPGRGTRITAAVDTEVEPAS"
     gene            23722..24939
                     /locus_tag="VFC01_27465"
     CDS             23722..24939
                     /locus_tag="VFC01_27465"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN83390.1"
                     /translation="MSTVSSPAGQRSTARAQMLSRLAHSGRDVMCYTVGDERNILLNH
                     PDHVEHVLAANRANYSKDTAANRYFRTEIADGILTADGDGWRRQRTLLNPVFRDRARL
                     ATAAREAIAALVARLDKLADTGRELNLSAAIGEVTLSITVRALFGMSHEPFIDQCIAL
                     GGALDDANSLLPGMDGAGPRDGLYRQLRAALSASARGEYGPALAALADDPDHSGEALY
                     QQLVTLLLAGFETTANSLTWAWILLMQHPDVYEEWQHSLGTSAALTKALFDETLRLYP
                     SAWILGRRALSDDHVGGVDIPAGAAVTISPFLLHRHPKFWDDAEAFRPHRFLPGGARP
                     AHRYAYIPFGAGHRYCIGATYAHDEAEMILDELGRRFTFRSEHVADARPEHKFVLRAP
                     DPLRVTVHRRNTP"
     gene            24936..25205
                     /locus_tag="VFC01_27470"
     CDS             24936..25205
                     /locus_tag="VFC01_27470"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZN83391.1"
                     /translation="MTIAPASPTEFGAAVCALIAEHTNLPVDFITPDRAFDDLPLDSL
                     GMLELILLAEATFGIVALDDDGIEVHTVGDAITFLASKLPVAQND"
     gene            25216..26874
                     /locus_tag="VFC01_27475"
     CDS             25216..26874
                     /locus_tag="VFC01_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HZN83392.1"
                     /translation="MSSLTDQMYANAATSPRGMITGTFEDPIRRSWGEVHDEARRMAG
                     ALSKMGVEPGASVAVLAGDPADVAPLAQAVWMRGASLTMLAQPTPRTDLAMWLADTHR
                     AARTVRAALTIVGAPFTEAAEPLNAAGQDVVMLEELRTGPAIEPLHPDESAIAMYQLT
                     SGSTGTPKAVQLTHANIVANSNALFTATDGDAATDVMVSWLPLSHDMGMLAFLALPMQ
                     YGVELVASRPEEFLRRPALWADLISRYRGTITSGPNFAYALLALTLERADPGRFDLSS
                     LRVAINGAEPIDARDTRRLAEAGARFGLRPGAITPAYGMAEATLAITFDNSPQVNVDR
                     ISRIDLQERALATPDAGADACGVVSVGLPVAGMEIRVVDYYSRPRPPRHVGSIEVRGA
                     AVSGGYLADQGWREGAFIRDGWLHTGDLGYFDEHGRLYVCGRTKDVIVIAGNNIYPTD
                     VERAACAVPGVRAGNAVAVRIDAGEFREGFAVLAESKLAADPVEAERIRAEIALRVNQ
                     AVGPNARAVLVLPPGSVPKTGSGKLQRRKAAELFARLDAEAVPA"
     gene            26871..27686
                     /locus_tag="VFC01_27480"
     CDS             26871..27686
                     /locus_tag="VFC01_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN83393.1"
                     /translation="MSPKATTSEDRSEERARTGARRSRGISKAPAIELERLRIVRGGN
                     TVIDDVTVSVSEGIITGLLGPSGCGKTTLMRTIVGNQKITAGSATVLGHAAGAKSLRR
                     HVGYVTQSPSIYRDLTTIENVRYFAALYGIGMQRVHEVVEAVGLTGQKTDLAGNLSGG
                     QQGRVSLACALVCDPDLLILDEPTVGLDPLLRVELWEQFVDMADRGKTLLISSHAMDE
                     ARQCAELILMRDGAILAHAEPEAILRQTNCLDLESAFVALVRSELQPVEEVRS"
     gene            27683..28438
                     /locus_tag="VFC01_27485"
     CDS             27683..28438
                     /locus_tag="VFC01_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019665793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN83394.1"
                     /translation="MNPTTHWATTVRILTQLRRDRRAMFMILVIPALITVLFYSTLSL
                     QGPGPDGLTPYDRLGIFQIAVIPCAVMFPIAAITMRRERASGTLDRLFTTPMSKLDLL
                     AGYGTAFSLGAVAQAVIVTLVAYRVTDLSTAGSPLLVAMVCVLTAVLGVALGLFFSAF
                     AETEFQAVQFAPIVLVPQLFLAGVFIPRDEMPGWLHSFSDVLPLTYVVEALQQISANA
                     EATPRMWRDVGVVVAFIIAAVLGGAATLRRQEA"
     gene            28442..29233
                     /locus_tag="VFC01_27490"
     CDS             28442..29233
                     /locus_tag="VFC01_27490"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83395.1"
                     /translation="MYTFAHDGVTFTVQPAEQGRHTVVFVNGLFGGGWIWEPVVATLT
                     AQGYGTVVTAEPLAAHETAEDIPALLQSISLLVDTLPDAAPILCGNSLGALVAMELAA
                     AEPSRWAGVILSGAPGLGDEQDADSFGSALRTPSLRLGDLIADRLIHDKELITPELVE
                     RCTQALTPRIMLRAGRALRATRGYDARGLLGRIDSPVLLICGACDEISSPAKWRDAAP
                     MFPDADYVEIAGAGHSPMLEKPDEFSALVTAWLDTIPAPHLSGTG"
     gene            29263..29526
                     /locus_tag="VFC01_27495"
     CDS             29263..29526
                     /locus_tag="VFC01_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZN83396.1"
                     /translation="MDCTLSAYDTLVDWLIDRIAVYLATSPAAIETDKTFGDYGLDSA
                     FALNLCTDLETELGILVEPTIAWDYPTIGDIAGHLAESTGRTR"
     gene            29523..32483
                     /locus_tag="VFC01_27500"
     CDS             29523..32483
                     /locus_tag="VFC01_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HZN83397.1"
                     /translation="MTPIASQIAVVGMDCRFPCATDIDEMWRLLMAGEVAKSVVPADR
                     WDIDRFHSTQIRPGTMNTRYAHFIDDADLFDHEFFGISPLEAAALDPQQRLVLQSVWR
                     ALEDAAIDPRALAGTEAGVFVGMMLSEWGALNLLDFPSLTSQRGIGGGHSMVANRVSY
                     HLNLTGPSVTVDTACSSSLTAVHLGCQALASGDTDLVVASGVNILLTPALSIFYTQAG
                     LSAPDGTCKPFSANADGIGRGEGVGTVVLRRLDDAVADRQPIYSVIAGSATNQDGKSS
                     GITAPNRWAQSKVMQRALDRAGAAASDVTFVEAHGTGTTLGDMIEANALGDLHRTGRG
                     APCLLGSIKGNIGHAEGAAGIASLIKTSLALSRGLLPPTVAAAGPNPALRLDQQGLTL
                     ATTAAELGDGPVLAGVSSFGIGGSNVHVLLTSPPAVTAVPEVHDDDEVGVITVSAHSS
                     DALRRNATALANALDENNDDAVAALCYTSNQVKSSLKHRFAAAGTRNELIGALLGYDM
                     SARTEKSPRRGGRLRIGLLCTGQGAQYPGMTQALYRTCPPYREHLTRAAAAVDTTFAG
                     PNGLLTNMFRGGDVIDTTEYAQPALFAVSYAIGAALLDLGVEPTFLIGHSVGEFAAAA
                     LAEVLTLDEAAGLVVARGAVMQQLPKGGAMSAVDVPAEQVANLVAEEPTCGIAAINGP
                     ASTVVSGPAAAVDRITTALARGGAKVTSLPVSHAFHSPLMAPAQDAFRAIAGAVTPRK
                     ATIPIISTVHGTIVDGTDMDADYWAAQLTSTVRFMDAMRTATTQASPTHLVELGPRTT
                     LLAHARRGGIDMDVATLAPCVGPDDDGAGFARVVARLYSDGLTTQFDHLYDAESRTLK
                     RLPPYVFGDGSRFWRVLDTAAVTGSVSTATSVVATEPGPASEIADAAPLASRIRQLIA
                     DIGGYTVEQIDPNALLGADLGYDSLLQLRLIESIRTEYPELQEAPVSELLSAIENVDD
                     VVRYVADRLAEVAR"
     gene            32480..34159
                     /locus_tag="VFC01_27505"
     CDS             32480..34159
                     /locus_tag="VFC01_27505"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HZN83398.1"
                     /translation="MTIVDIVAARATSESLAYVYLADGTPASELRWSFADTASRTRAY
                     AAHLRDRGISAGHRVVLAINPSLDYIAALYGIMWLGAIPVPCFPPLRTKELDRFGAIT
                     VDCAPTAIVIDEMYREPIEALRRRLESVGLEPTVVYVEQVDDAGDDSPPVPTAARDIA
                     LIQYTSGSTGTPKGVCLTHANLVSNCEALGRNMGHDPDRIGFSWLPPYHDMGLMGTIL
                     VSMFQGVPLVTMSPLHFVQSPRRWLEAITQYKVTIAVGPNFSLDMCSDVLADGDNTDL
                     DLSTLERLYCGAEPISIDTLARFEKAAAPLGFNLDALVPCYGMAETTLYVSGKSPGRN
                     YHTEPSPEPSALPRDVVSCGKVDPEHTVRIVEPTSLEALSDGRIGEIWVAGPSVAAGY
                     HDRHYLTRRVFGARIAGELGGYLRTGDLGFVRSGELFVTGRIKDLIIVNGRNIYPQDV
                     EANVGRADPAVRIAVAFSIPGDNSEQLCVIAEVGQPQVTAQVHARLVDAIRASVTSEF
                     GIRPHVHIGPKRTVPTTTSGKVRRQETRRMFLADELLSVPIDSPATQECTA"
     gene            34156..35097
                     /locus_tag="VFC01_27510"
     CDS             34156..35097
                     /locus_tag="VFC01_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HZN83399.1"
                     /translation="MSQLASTRHPAPNEQAPLPERDVLASRLAYLTVGIPTLGTVAAV
                     GYAISDGLWLSDVLVFLAMYLITGLGVEGGLHRYFTHRSFVASEPVKVVLAVAGSMAA
                     QGPVIFWVANHRLHHAFADTDRDPHSPQPQGPGWWGRVKGLWHGHIGWLFTVNKIDWS
                     RHTRDWLQDRRVMKINGHYFTVVLAGIVIPGLVGGLATQSMHGFVGGILWGGFVRIFA
                     LDHATWVVNSLGHTIGNREFHIRDQSRNIGVLAPPTMGGSWHNNHHARPSLAQTRRHW
                     WQLDLVGDFIGLLDRVGLVSNVRYPNTQEENHDDRNS"
     gene            35078..36088
                     /locus_tag="VFC01_27515"
     CDS             35078..36088
                     /locus_tag="VFC01_27515"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HZN83400.1"
                     /translation="MMIETASPTTASDSRQESLARAIVQLNPKSARAKRVVAAVTVGI
                     PAVGFAIAVYLGVAGTATALDYLLFAVFYVIQMFGVSIGFHRYLAHNSFKTSPFFEGV
                     LMVTGSMALEGPVLFWVSTHRRHHRYSDELGDPHSPNLSGSGLAGKLKGLWYAHIPWM
                     FSDQESRVTVFAPDVVRDRRLYFYNRTYPVWALTSLLLPALLGFTIRGTAAAAFSGFI
                     FGGLARVFVANQAAWCVGSVSHMIGSRPFATRDDSANNWPVAILTFGEGLQNNHHAFP
                     GAYRHAMRWWEPDLSGWVIAILAKAGIVWSLKMPDRQKINSRLRRNRPSVKSTDELRK
                     EG"
     gene            36092..36382
                     /locus_tag="VFC01_27520"
     CDS             36092..36382
                     /locus_tag="VFC01_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZN83401.1"
                     /translation="MFGRNRTATIDGPLTHEKLRSWLIAELARMVKIDQAQVDTAKPF
                     QAYGLDSRAAVQVSGALEKVVERRLSPGILFDHETIDELADHLVGELQLERG"
     gene            36399..37484
                     /locus_tag="VFC01_27525"
     CDS             36399..37484
                     /locus_tag="VFC01_27525"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZN83402.1"
                     /translation="MTKFVTLTDPDPRFTEFIEKSNTLEGAKLAAAIPKEYVQPSLAR
                     GLLGLAASLSVYVGAIVAMAVLDRWYLVVPLAILAGLGGWGLHCIAHECGHGSFSRNR
                     RLNFVIGHLTLVHLLYPFHAWRHVHNLHHAHTNSLELDTDWAPIPEAVYHRMALRDRM
                     IYISVRTWAVWGGTIHHWLQSGLRPSYFPKREMRRDARRSMAFVAVVGGAYLAALTYF
                     TGWQGLLLYFVAPWFATHVWFSITTLMHHSAEDIPYLTSEHWTRNASKLLVTTDFVYP
                     RWLLFLTHNISLHTAHHVSPAVPFYHLGKAQAALLQAYPEMMRVEKASARKLWNILRN
                     CRFYDPVSGLYSTRPMRRVVPQQKESA"
     gene            37481..38317
                     /locus_tag="VFC01_27530"
     CDS             37481..38317
                     /locus_tag="VFC01_27530"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83403.1"
                     /translation="MSAGLFVAQALTRAHRIAPRAAGYVWTSIFTNTRGLGTAPNDLC
                     PLGASRVELTGVPRVAAVYVWGSAEPTVLALHGWGADSTTMSSVVAAAADSGESAVCF
                     DAPGHGASPGTQATMREYSDATLAVLQRFPSITTIVAHSFAAIAAVSAVAAAAGNSVR
                     FLLLLAPACSLSDVLERWAAHRDLPPAVVEQIRRELRRRDGMEVSHWDVRTLGLPASV
                     RVQILHDPADALVPVSDSYRIAASISAEIQATMPGVGHHRVISCDEMRRALTARATAS
                     DR"
     gene            38553..39134
                     /locus_tag="VFC01_27535"
     CDS             38553..39134
                     /locus_tag="VFC01_27535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83404.1"
                     /translation="MTSGLRTPLGPASATLILGGDAQTLSWSFVATDFPFLAASGSAQ
                     AKAISAATGNKDLFSATVLKVSHHASKHGVNLELVERIHPAVTLVSSTASGTKYGFPH
                     TVSQEAIREALDPVASKLNPATGLPKDHKSDAELRIFLHQRYHHGTGRPRCWILRPIA
                     ARRASRALALRRCSQPIGRPQQCQAVDVKPDPR"
     gene            complement(39148..40167)
                     /locus_tag="VFC01_27540"
     CDS             complement(39148..40167)
                     /locus_tag="VFC01_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019739010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071768 - mycolic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused (3R)-hydroxyacyl-ACP dehydratase subunits
                     HadA/HadB"
                     /protein_id="HZN83405.1"
                     /translation="MTAPEASLLEARVGHYYQMDGTYLVGREKVREYARAVQDYHPAH
                     WDVAAAAELGYSDLMAPLTFTSTPGMTCNRHMFEQVVVGYDTYMQTEEVFEQHRPIVA
                     GDELHVDVELTSVRRIAGRDLITVTNTFTDTAGERVHTLHTTVVGVTAEDLNPEIKTA
                     VHNAMMHDVDIFGVGESEYHKTVRPEGEVRIADSTARTPGTPSFDDVKVGDELPVHHT
                     RLSRGDLVNYAGVAGDANPIHWDEAIAKLAGLPDVIAHGMLTMGLGAGFASAWSGDPG
                     AVTRYAVRLSAPAIVSATGGADIEFGGRIKSLDPETRSGVVIVSAKSDGKKIFGLATL
                     DVRFR"
     gene            40256..41281
                     /locus_tag="VFC01_27545"
     CDS             40256..41281
                     /locus_tag="VFC01_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HZN83406.1"
                     /translation="MARSTNFPEVMSSAAGAVARWIAPLLTVAAVACVGLVAHSVPAP
                     AVRLAADEGNPLAGLPFFVNPTSAAMRAAQKADPPSPELTAIANTPQAYWLVPGTSAA
                     TVAKYTGDAQAAGAMPVLALYGIPHRDCGSFAAGGFATGAEYRQWIDGIAADLGSSPA
                     AVIVEPDALAMADCLSGDQRQERFDLIRYAVDTLTRNPAAAVYIDAGHLRWHSADEMA
                     ARLNQAGVDHARGFSLNTANFFTTDDEIGYGEAISGLTNGSHYVIDTSRNGAGPAPDS
                     PLDWCNPSGRALGTPPTTATAGAHADAYLWIKRPGESDGSCNRGDPPAGNFVTQFAIA
                     LASNPGH"
     gene            41302..42498
                     /locus_tag="VFC01_27550"
     CDS             41302..42498
                     /locus_tag="VFC01_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZN83407.1"
                     /translation="MTDNPAEVVESVEPRRSMRHYWRRMAIVLTITLLLFGVPWWTLF
                     APSASWPTAVFVVGTLLFVGAFAAMPLLMVFGHGRRHLDWAATTGDTLLGAAWVMFVW
                     SLLGQLLRLVLLTAGVADPVRSRLVAGAVLCAVVVLLAWGYAEAMRVPRVKKVEVAIA
                     RLGPGLDGLRVAVITDTHYGPINRARWSTAVVARVNELSADLVCHVGDIADGTVEVRE
                     LQASPLASVNATLARVYVTGNHEYFSEAEGWLDYMESIGWAALHNRHLIVERGGDRLV
                     VAGVDDATAQSSDARGHRADLEAARAGADATLPVLLLAHQPKQVSHAMRAGVDLQISG
                     HTHGGQIWPFNLLVRLEQPVVHGLSRHGDRTQLYTSRGTGFWGPPFRVFAPSEITLLT
                     LRSPLR"
     gene            42673..43812
                     /locus_tag="VFC01_27555"
     CDS             42673..43812
                     /locus_tag="VFC01_27555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83408.1"
                     /translation="MSMNINYEPAASRQFEANPPFFGSKVASITTPGWQKNPVGSHLI
                     MTVGVFAFCGVLGGLYMFMQSSVERSSTSMQQFAGYGFLAVGIALLVLGTAIWYRRSQ
                     RKILLSVTTDELTVNIRPGDVYSFIGAKLGTWGVTGGATMGLALHLQSGERRFILGGR
                     DRRVAAGTRLEAEDVGYGLPVDVDAWLSAEDFEEILTMVSRRSGLDIRRPGPGDPIRC
                     LLFVNSLKLQEIGSFSFRKQWEFSRSMHQARLAIDVVPEGIRVIDPQSGALIAAVSTA
                     QVTATPVIYSPEQGNHWFPDLGNVISDVATNYWSTSPGMRVSVPGMAPLTIGCRDSVS
                     GLDFRFSWPGGVPVVAARADYEISGTDLLTLTEKFGLTRHLQRGA"
     gene            complement(43815..46127)
                     /locus_tag="VFC01_27560"
     CDS             complement(43815..46127)
                     /locus_tag="VFC01_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HZN83409.1"
                     /translation="MKSVNARLAEELAVQEVQVAAAVRLLDEGATVPFIARYRKEATG
                     SLDDGRLRSLEERLQYLRELDDRRAAVLASIDERGKLTDELKAALMAADTKSRVEDIY
                     LPYKPKRRTKAQIAREAGLEPLADRLLADPTLVPEEAAAEFLRDDVADASAALEGARH
                     ILAERAAEDAELIGAIREKFWKAGSLRTAPWSDDVAKGAAAQKFRDYFEFGESLETMP
                     SHRVLAVMRGEKEEALALTFDGGDDEFYHAMIAQTLGIDMTSNASATPWLAGTVAWAW
                     RTKLNISARVDARVRLRQRAEEDAVAVFAKNLKDLLLAAPAGNRTTLGLDPGYRTGVK
                     VAVVDGTGKVVDTCAIFPHQPQKQWDSAKAALGALVARHGVELIAVGNGTASRETDAL
                     AAELIADIRAAGAKAPAKAMVSEAGASVYSASAYAAQELPSLDVTLRGAVSIARRLQD
                     PLAELVKIEPKSIGVGQYQHDVTPGTLARSLEAVVEDAVNAVGVDLNTASVPLLARVS
                     GVSESLAEAIVAHRDKTGPFRNRKALLKVPRLGPKAFEQCAGFLRIRGGDDPIDASGV
                     HPEAYPVVRRILDRAGVTLAELIGNERSLRALRPADFADDQFGMPTVTDILAELEKPG
                     RDPRPAFSTATFAAGVEKVADLKVGMVLEGVVTNVAAFGAFVDVGVHQDGLVHVSAMA
                     DHFVSDPHEVVRSGQVVRVKVVAVDVERQRIGLSLRLNDTPQQDRGGRREPDKQRQGK
                     PRRDKDAGRRPSNQPAGSMADALRKAGFGS"
     gene            complement(46320..46550)
                     /locus_tag="VFC01_27565"
     CDS             complement(46320..46550)
                     /locus_tag="VFC01_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83410.1"
                     /translation="MTDKNTDTSVAVDMRVRVYPGTDAECRGVVVDDFGQTAGHPVDI
                     GTERIADPARRWAVRLDTGSLVFTDSDQLIPE"
     gene            46761..47168
                     /locus_tag="VFC01_27570"
     CDS             46761..47168
                     /locus_tag="VFC01_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZN83411.1"
                     /translation="MAKYLFLKHYRGAPASVNDVPMDQWTPDEVQAHIQYMNDFAKRL
                     QESGEFVDSQALAPDGAWVRSDGEGKPPVTDGPFAETKDLIAGWMIIDVDSYERAVEL
                     AGELSAAPGAGGKPIHEWLEVRSFLTAAPKFTD"
     gene            47193..>47542
                     /locus_tag="VFC01_27575"
     CDS             47193..>47542
                     /locus_tag="VFC01_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HZN83412.1"
                     /translation="MDEAQLRDLIPGVLAALVHRGADFATAEDAVQEALVRAVETWPN
                     QRPDDAKGWLITTAWRRFLDLTRSDIARRRREAQVSDEPPTGPTASVDDTLQLYFLCA
                     HPSLTPASAVALTLR"
BASE COUNT         8333 a        15733 c        15033 g         8443 t
ORIGIN      
        1 ctcgcgcgcg gcggcaacga gcagcgcgac gatgtcctgg gcggtcaccg agtccaggct
       61 tccggtgggc tcgtcggcca gaacgaccgc gggttcggcc acgagggctc tcgcgatcgc
      121 cacccgttgc tgctggccgc ctgagagttg gtcggtcagc ttcaccgagt cctgcgcgag
      181 tcctactcgt tcgagcgcgc tggccacccg gcgatcccgt accgtcggct cgattccgtc
      241 gagaagcaat ggcacttcga cgttttcggc cgccgtcgcc tgcggaagaa gcgccaggcc
      301 ttgaacgatg aaaccgcacg ttcgccggcg aaatcgggcg cgggcacggc cgcgcagcgt
      361 cgcccagtca acgccggtca cccgcacgtg tccctcgttg ggggtgtcga gtccgccgag
      421 aagatagaga agggtgcttt tgcctgagcc gctcgggccg gtaacagcta tgacctcgcc
      481 ccgggcggcg gcgagcgaca ccgaatcgac accgcgaacc tcgtgcccgc cgcctcgata
      541 cgtccgggtc aacgtgagcg cctcgatcac gggcgcttca ggcaccatcg aggatctcgc
      601 cgtcgcgcat gctgaggcgc cggtcggccc gctcggcgac ctgcgggttg tgcgtcaccg
      661 tgaggatgct cgcgccctcc cccgccttga gagtcgccag aaggtcgaga acgcctgcgg
      721 ccgttgactc gtcgagctca ccggtcggct cgtcggccag cagcacctgc gggtgcggtg
      781 ccagggcgac ggcgatggct gctcgttgcc cctcaccgcc ggacaactgc cctgcccggt
      841 ggcggcgccg gtcggcgagc ccgacctcgt ccagaagttg gtccacccgg gcccggatct
      901 gtgtgcgccc tcgaccctcg aggcgcagcg gcagctcgat gttctcggcg acgtcgagaa
      961 gcgggtgcag gttgtcccgt tggagtacca cgccgacccg cccacggcgc caccgagccc
     1021 gcgcgggcgg cggtagccgg gtgaggtctt cgccgtcgac gacgaccgct cctccgctgg
     1081 gctcgacgag cccggccaac acgtagacga gggtgctctt gccgcttccc gacggtccca
     1141 tgacgctggt ccaggtgccc ggctccagtg tcaactccgc gcctcgaagc gcgatggtct
     1201 cgacctcggt ctcgcggtag atgtggtaca gcccgcgggc ttccatcgcg gggccggttc
     1261 ggtcggtcac ttcggcgcca ggatggagac cggccacacg tcttcgcggt ggccgttcac
     1321 gagcccgccg agcttgacga gcccgacgcc tggggcgaag tatttgtctt cgtccttgtt
     1381 caggtccagc aggtctcggt cgttggtcac cagcacgtcg gaatagcgac ccgctgggac
     1441 ctcggccgag gcgccgagtt gcttgatgag ggccacgtcc tctgcgaccg tcggccggta
     1501 ctcctgtcga tacgcgtcgc cctgcacggg gcgggcctgc atgatgatgc cgggcagcgc
     1561 tccgtcgacg cccgcctccc acgaaccggc ggtgctagat acaaccccgt tcgtgtactc
     1621 ctttgtgtcc tcgccgaagt accagacgtt gcccgcgggg tcctgcgcgt accagtccga
     1681 tgtgcgctcc tcaagggctc ccgtgacgat gtcccgcacg acgctcgtct ttactccgaa
     1741 aatgtccttc gtctcgttgg tcaccgtcag ctcgatccgc agcggcaccc cgtcgcgctt
     1801 gccttcgtag accatcacgg tccccggaac gagcgggaag tacttgttgg tgatcttgtc
     1861 ggtgaactgg gccggatcga ccttggggga gtagggggtc tgcatcaacg gcaccgtcca
     1921 atccggcggc gacggtgccg ctgtggggct accggtgttg atcgaccccg gctgcccggg
     1981 cggggccgac gtcgagccat tcgaaccgca gccggccatc gcgaccgcgg ccataaaagc
     2041 gatgatgagc ctgcgcatcg cggtcacctc catcgataca ttcgccgtaa gcatgtgctg
     2101 ccattcctga acggcatctg aacgccagcc gggccggtta ccgattcctt ttcggggtgc
     2161 acccggtcaa tacaggtatg gacatagcgc tcaatgacgc cgcgcgcgaa gtgcccgacg
     2221 gcccacactt cgcggtgatc gccgcgaacg ccgacgggtg cccgcagtcg tcggtgatct
     2281 tcgtcaagcg gacggagagt gatcgtccag agggccacgg catgtgcgga taccacgccg
     2341 gcgaccgagc tccggagcgc cgacgtccgt ccggtgttca cgtctcggac cgcggcgggc
     2401 tgattcatct gatgaaatca acggtgaggt accaaggccg ctgacgcagt gactcgaaaa
     2461 gggttgcgcg aatgcatact acagatggaa ccgaattatc ggcgcgcgac gtgagagcga
     2521 aatacattgg ggtacatcat gataacattt ttgacagaaa cctcgttccg gagatctcga
     2581 ccgaggttgc cttgcgccag gtgtatctgg cgccaccggt aaattcgaag ttgcaccggc
     2641 cgccctgggg ctcttcagtg cgcacggcat cccaatcggc ctgcggcatt gtgatcttca
     2701 ccgtgattac gttgtcgagc gcataaaacg aatcgagtgc ttgctgctgt tcggcgctca
     2761 tcgttaatcc ccgtttccgc caccattcgt ggactacttc agggtgcgcc gggtgcgcgc
     2821 tttgagcgcg agtaatggac tactcgtctt ggacaagaat tctcaggtgt gcgcgccacg
     2881 aatattcctg aacctctggt catcagccag gctggccgac cggtttctct tgggctgtgc
     2941 tttcgccatg tgagttcacc agccgcatgg ctagcgccga aggcaagtga actggaattt
     3001 tgcagcgaag attgacgaga ctccgcgaca cgccggcgtt ttgtccaagt agcccggcgt
     3061 ttctgatcaa tcttcactgc atctcgacag cgttgctgct gccgcgcgcc gcgcggctgc
     3121 actgagcagc caccatgtgg gttctcaagc ttgactgcac tttctcaagt tttactgcac
     3181 caagactgca tttgtgcagt gagcgtgaga cgcgccgcag cacgtctggg ttcgtgagat
     3241 gaaatggcac ggcgcatgtt cggtggtgga tccgaatctt ggtcctagtc aagtggcgcc
     3301 aatcacggat cggaagactc aacgttggtg cgtgcgtcgt aggactgtcg ggcggtggtg
     3361 atgaagtcga ggttgtcgtt ggtccaggcg gcgagcgatt gcacgatagt cagcaggctt
     3421 cgccccacgg aggtcagcga gtattcaact cgcggaggca cgctgggatg gacggtgcga
     3481 gagacgatgc cgtcgcgttc tagattgcgc agggtcgaac tgagcatgcg ctgggtgatg
     3541 ccgccagaga gtcggcgtag ctcgttgaac cgtcgcggct caattccgag ctgacacaaa
     3601 atgatcaccg accacttgtc accgatccgc gccaaaacac ctcggaccag cgctgcccgc
     3661 tcatcgtcgc caagttcgcc ggcaccggta cccgcggtgt cggcaaggct ggttccctcg
     3721 gagataccag ggcactttga actgccctct tgctgcgtgg agggcgcagg ggtcaccatc
     3781 atccaaccag tatctctcag atacttagct gatggatggg cactagccag gagttggatt
     3841 aatgaaggct gtagccgtac ttgattacgg cggccccgac gtgctgcgtg tagtcgatgt
     3901 gcccgacccc cgcgccgggt cgggccagat tcggcttcgt gtgcacgccg cggcggtgaa
     3961 cccggcagac acgctgctgc gagtgggtga catcgatgcc gcacttgccg gcaacctcga
     4021 tccgccctat cggcccggta tggacgcggc ggggatcgtg catgaaatcg gtccagacac
     4081 cacgaccgat ctgcaggtgg gtgaccgggc gatggcgatg gtcgtgccta tcgacgcctc
     4141 gggtggcgct tacgccgaat atgttgttct gcaggcagat caagtcgcgc ctgcaccgca
     4201 ggggacaagc cacgtcgagg cagcgacgct gccgatgaat ggcctgaccg ccctgctcgc
     4261 ggtcgatacc ctcgatctgg accctggcgc ctggctcgcg gtgaccggcg cagtgggcgc
     4321 cgtcggcggc tacgtcgtcg agctcgccaa agccaggggt atcagagtcg tcgccgacgc
     4381 ctccccggcc gacgaggcac tcgtgcgcaa gctgggagcc gatctgattg tggcccgtgg
     4441 gcccgggttc ggtgaccgtg tgcacgaagc actccccgac ggtgccgcgg ccgtggctga
     4501 taccgcactg ttgggcgaag atgtcagcgc cgcgctccgt ccaggcggac agatcgcggt
     4561 gttccgcaga ccgggtgaac ggggcacgcg gccttacggg gcacccacag acgtcagttt
     4621 gcgcgacatt tgggtgcctg agtaccgccg ggcccgccac aagctcgact acctacgcgg
     4681 ccttgtggag caggggaaga tcacgctacg ggtcgccgac gtttatcccg ctgccgacgc
     4741 cccagcggcg caccgccgcc tggaggcggg cggcgtccgg ggacgcctcg tgttggatat
     4801 ctgggctccg caggagatgc aggagcccag ccagtgagtg gggccgtcga tatcggcgtc
     4861 ggaatcatcg gtgccagccc tggacgcagt tgggccgcca tgtcacacat ccctgcacta
     4921 caggcgttgt cgggataccg gctgcgcgcc gtagccaccg ggcgcgcggc ttctgccgag
     4981 cgtgccggaa cagagttcgg ggtggatggt ttcgacgacc cgcgggcgct gatcgaacac
     5041 cccttggtgg atctggtggt ggtggcggtc aaggtcactc accaccaaca gctggtgagc
     5101 caggcgttgg cggcaggcaa gatggtgttc tgtgaatggc cgctgggcgt tgacgcctac
     5161 gaggctcaaa gcatcaccga cgaagccgtg cgtgctgggg tacccaccgt ggtcgggctg
     5221 caatcccgct tcaccgcagg tgtgcgccag gcggctgaac tcgtgcgttc cggccacctg
     5281 gggagggtcg tctcaacgtc catcgtcggc tccgggctgg cgtggcgccg ggtcacagac
     5341 ccctcacacg cctacatgta cgacgggcga agcggtgcga caacgcttac cgtcccggct
     5401 gctcacgcat tggacgcact gagtttcgtc ctcggtgaaa ccatcaggta cacagcagcg
     5461 ttcggcttga gttcaccgta tgtcgaggta gccggtgagg ctgaagcgct gacagtgact
     5521 gctccggatc aaatcgccct gacggctgag ctcgcatcgg gagcagtcgc ctcggtgttc
     5581 taccgaggcg gctcatcgcg agtggcaaac ctgcggtggg aaatcaacgg aacagacggc
     5641 gacctgttgc tcaccagccc gtctggaaac ggcaacatcc aaatcggcga tctcgcactg
     5701 gccgcaggcc actgtaccga caccaagatc gtcgagatcg aattaccttc acccacagcc
     5761 gaagtcgtca atgttccgcc cgggccggcg gtggccgtcg ccgaactgta tgcagcgttc
     5821 gcccgcgacc tccgcaacgg cacccaggac gctccgacgt tcgtcaccgc actgcagtta
     5881 caccgcgcgt tagcaggcat cgagcgcggg gcgcgctgga catcgccagt cgaggtgcgc
     5941 tcgacaccgt aggtgggggc agtgcgccga agagacacga ctaggcgtcg ctctcttctg
     6001 gtgatgcgcg gagtggcgtg gacggccgtt gacgtaggcc gggagttgag gcgcttaccg
     6061 gccatcgggg acaacgagtt tcgcctttaa cgacaaccga gaggaaatca ccatgggaca
     6121 actcgaaggc aaggtagcgc tcgtcaccgg aggaagcgcc ggtatcgggc tggagacagc
     6181 tcgccgcttc gccgcagaag gtgcgcaggt ctacctgacc gggcgccgca aggacgtggt
     6241 tgatgccgcc gtctccgaca tcgacggcga cgtcgttggt gtcgtgggcg acgtcgccga
     6301 cctgaccgcc cttgacgcgc tgtacgctac gatcgccgaa cgcagcggcc gcctcgacgt
     6361 cctggtcgcc aacgccggcg gcggacaagt ggctgggttg gctgacatca ccgaagagca
     6421 ctacgacacg accttcgacg ccaatgtcaa aggcttggtg ttcaccgtgc agaaggcgct
     6481 accgctcctg caggacggtg catcgatcgt cttgctgtcc tcggtcgccg gcatcaaagg
     6541 ccttgctggc gtgagtgtgt acgcggcaac caaagctgcg atccgtaatt tcgcgcgctc
     6601 ctgggcggtc gaactggcac cgcgcggcat ccgtgtcact gcgctgtgcc cgggacccgt
     6661 tgacacaccc ggcttggctg gattcgccgc acaagtcccc agcgacgacg ccgcctccac
     6721 gatcgcaata ccgatgggcc gtgttggcga tcccaaagag attgccgcag gcgcgttgtt
     6781 cctcgcctcg aatgagtcgt cgttcctcac cggttcggac ctgatggtcg acggcggcca
     6841 agctgcggcc tgacctgaaa ttcggccttt cctcgatcac ctcggaattc gaccagtacg
     6901 tcgacgaatg atggacgcga atctgcccat ggcgacaccc gccgcattga acgattgccc
     6961 tacagatatg ccagacgaac tcgggcgcgc gttcagccaa gaccgcgacg tccgggtaag
     7021 acgtgctctc gcaagcgcat tagataacgc agaacatcgg cagcgcgcag atgctcgaga
     7081 aatactcctg cacgatccgc ggtggtccgt ccgctcaatt ctgcacactg ctacacaccc
     7141 gcctgactga gagcggttct cgctcatgat cacgccttcg acccgggtgc ccatccacct
     7201 tgactagcgg cccctcatag tctgacgaga cgcatgagtt ttcctgtctt gaactgcgta
     7261 tcaccatcga gatccgacga gtccacgtgt aggtactcgc gaacggtggc ggaacttcac
     7321 gtgtgatggc ggagcgatgg cagatccgtc aagccatcga cggcgtgacc ttggccggcg
     7381 tgatcttgcg gtactgatat ctgtggagct cggtcgcgtc ccccgcgaag cgtgaccgat
     7441 cctgcaacga caccgaactg gccgactaaa ctgagctaac ccggccagcc tgtctccagt
     7501 gggaactgtc cgtcggtcaa atggcgcttc agtatctttc ccgtcgcggt gcgcggcagc
     7561 tgattgacga acgtgacgtc gcgcggaatc gagaagcgac tcagacggtt ccggacgtaa
     7621 tccttgacca tctcttcgtc cagcgacgcc ccgtcggcgc ggaccacgaa cgcggcgagg
     7681 cgttgaccga attccggatc gggaacgccg accactgcca cgtccgcgac ctgtggcaag
     7741 acggcgatag cttcttcgac aggccgcgga aacacgttct cgccgccgga gatgatcatc
     7801 tcgtcgtcgc gtccggcgac gaagagccgc ccgctgacgt cgaggtaccc gagatcgccg
     7861 gtgtccatca gctggtcttc gatggcaggt gacgccgcgt tggtgtagcc gtcgaacagc
     7921 atgtcgttgc cgacgaagat gcgaccgacg gcgcccaccg gcagaggatt gccgtcctga
     7981 ccgagtatgg cgatgcgagt accgaggggt ggtcgacctg cggtcgtcgg ggcgacgcgc
     8041 aggtcggtgg gatcggcgat cgttgcccac gacacctcgg tggagccgta gaagttgtac
     8101 agaatatcgc cgaaggtgtc catgaacttg acgacggatg cgccggaaat cggcgacccg
     8161 ctgctggcga ccaccctcag cgacgacgtg tcgtatcggt cgaccacggc ggcaggcaac
     8221 tcaagcatgc gctgcaacat cactggaacc aatatcagga cggtgcagcg ctgcagcgca
     8281 atggttttga gacactcttc ggcgtcgaac cgttccatca gcaccaccgt tgcgagtagc
     8341 ggagcactca attgcagcgc ggcgaggccc caggagtgaa acagcggcgc agacagcaac
     8401 atcacttcgt cgcgacgcag cggcatccgc gacaacatcg cggcgattgc gccgaagccg
     8461 ggtggcgtgg gtcggcgcgc gcccttgggc gtgccagtgg tacccgaggt gagaacgatc
     8521 agcttgccgg gtcgtttcgg cggcgccacc ggttccgcgc cggcgctggc agagatgaga
     8581 tcgtcgatcg aacgccgctg cggcactatc gaattggggt gcgtcgaaat tcgcggaact
     8641 tccgcgggga gatagcgcac ctgcggctcg aggtcgtcgt cgacgaatat ggcgtcgacc
     8701 gggttgcgtt tgacgatctc gtcgatcgcg cgggctgcaa ggccggtgtt gagcaacacc
     8761 aggtctgcac ccaacttgct cgcagccacc atgcactcga ccatcgctga atgattgcgt
     8821 gcgaggaccg cgaacttgct gtctccggtg aagccgcctg cgacgaggcc cgacgctata
     8881 catgcggtgc gctcggccat gtccgcgtaa gtcgtcgtcc cgtaccgatc gaccgaagct
     8941 acgttgtgcg gtgatcgcgc ggcggccgcc ccgagaccgc ctgcgagcgt aaatccccac
     9001 ttggcaaggg aattgagttg ccgaagacca cgatccggcc gggacgctcg caccacaccg
     9061 ctgacgatca tggtcttcaa gacgctgagt tgcagagaat gcccgtcgcc catgttgacc
     9121 agcagtgacg actgcttgcc ttcgagatag tccgaaatac gcagcaggcc ttctctaacc
     9181 cagttccgga cggcgccgtc gctggctttc gcgaggtccg ggtggagaag gccgaccccg
     9241 aagatcctca ccgcgatccg agtgccgccc tcttcggggg tcaggcgaat cgacacgaaa
     9301 cagtgactca cctgtgttgc gtggaaccac atcagcatgc tcgaccggcg gagggtctgg
     9361 cgcatctcgt gagcgatgac ggcgccgcgg ggtgtggaca gacggacctc gaactcctgc
     9421 gcgtcgccgg aaacctgctc gcacgacccg agtccgcgga agaatcgcgg gtaccagtcg
     9481 ggtgcctgca ggatgcccca gatttccaga catgagtgat ccagtgtcat gtcgaaagcg
     9541 atcacgtcac gtgccacgtt ggtcctgcca ggctgttcgt aggccaactg agagatcaaa
     9601 ttgtggttca tccgccctcg attcggacct gtttcgtcga ctggaccgct ggcggggtgc
     9661 tcggtgcgca gcccagattg acaggctcct acggcacgcc ggggtcaaaa gtagccaggc
     9721 gcgtcttgtc aatctgcgct gcagctcgac accgctgcca cggcgtggag gactactggc
     9781 gctatcacca ccgcagatca gcctgatgaa taacgattcg aaacctgttg tgttgcagga
     9841 tgagacatga gcagtgacgt tttggccaca gacattatgg tcacgtggtg caccggcgaa
     9901 ccgccctcaa gatcccgctg tacctggcag cagccgcggc actgaccaag ttgcccctcg
     9961 tctctgctga gacgggccga tggtcggctg accgcgccaa cgcctggtat gcaaaccagg
    10021 ggtggctgct tggcgccaac tacgtcacct cgaacgccgt gaaccaactg gagatgttcc
    10081 aggcgggcac ctttgacccg cgccgcatcg cgggcgagct cagtgtggcc aagcaaattg
    10141 gaatgaacac catgcgggtg tttctccacg accaattgtg ggctgccgac cgggcgggat
    10201 tcagcgaccg gctttcccag ttcgtcgcca tcgcggcgag tcacaacatc aaaccgctct
    10261 tcgtcctgtt cgactcgtgc tgggacccgt tgcccagggc gggccgtcag cgcgcgccaa
    10321 tagcaggagt gcacaactcc ggttgggtgc agagcccagg cgcctaccgt ctacaggacc
    10381 cggcctacac ccgcgtgctg cagagctacg tcaccggagt ggtgggcctg ttccgcaacg
    10441 atcctcgcgt ccttggttgg gacgtatgga atgaaccgga caatccgtcg cgcgattacc
    10501 gcaaggttga acacgaggac aagcagcaac tggttgccgc gttcctccca cacgtgttcc
    10561 agtgggtgcg cgccgtcaac ccgattcaac cgttgaccag cggcgtatgg cagggccact
    10621 ggaaagaccc cggaagccgc agcgcgatct gcagcctgca gctcgagcac tcggacgtca
    10681 tcagcttcca caactacggg gatccggccg aattcgaggc ccgcatcgac gaactcactc
    10741 ctctgggacg acccatcctc tgcaccgagt atctggcgcg gcgcctgggc agcactgtgg
    10801 aaggaattct tccggtcgcc aagaggcgca acgtcggcgc ctacaactgg ggcttcgtcg
    10861 ctgggcggac gcagacgtat ctgccgtggg attcctggaa gcgcccgtac acctcgattc
    10921 ccgaaacgtg gttcagcgat ctaattcatc ccgacggtcg gccacacgac gacaacgaga
    10981 ttcggttcat ccgacagctc gctggagcag gtcgccccac ctgagggcga aacgctcgat
    11041 tagacgtcta gacacaggcg acgactggac ggaattcgaa tggatcgacg cggcggcgaa
    11101 ggtgcgctga gccctgggag atctgctagc tcgcgacgtc gaagtcttca ttgtggagcc
    11161 tctcgagccg ctaagtggac tcgacagccg atgagttttg cgcagtcgcg gggtctacat
    11221 ggattgcaaa gtgccggttc ggcacgacac gagaacgtca gaagaggagg ctcgtaccta
    11281 tggtcatcgt tgcaggacac atcaccgtcg agccccagca acaacgccga cccgacatcg
    11341 ccccttgacc acaccacgct caccgcggac agatcgtgtg cgcctcaacc cccgggagcc
    11401 aacagccatg tcttctcaca ccacctccac catcttcatc atcggcggca caggggcgca
    11461 aggaatgccc atcgtcgccg ccctggtcgc cgacaagaag tactccgtgc gcgtcctcac
    11521 ccgcgacgcg agctcccccc gagcccaggc cctccttgcg ctcggcaacg tctccatgct
    11581 cgaaggctcg ttcgccgacg aagccatcct gcgaaaagga ttccgcggct gcgacggggc
    11641 ctacgtcaac atcgacgggt tcaacaccgg cgagaagacc gagacctact gggcaatccg
    11701 cagctacgag atagccatcg aagaaggaat caagttcttc gtctacggaa accttgacta
    11761 cgccctcaag aaatcaggct acgactccaa gttccgcagc ggccactacg acggcaaagg
    11821 ccgtatgggc gaatggatcc tgtttcagaa tcaggacaac agcgacagga tgggtgcagc
    11881 ggtcttcacc tccggtccct acatcgagat ggtgatctca cccctgacgg ccatgacacc
    11941 caccattgaa gacggcgtcg tcacgtggcg ggtaccgctc ggccagggcg ccattcccca
    12001 tgtcgctctt gaggattgcg gatactacgc gcgctggatc ttcgaccacc ccgagcgctc
    12061 gaacggcatg aatctcgaag tggcgatcgc acacatgacc tacaccgcca tcgccgaggc
    12121 attccagaaa gtcaccgggc acccggccca atacatcgac accgatctgg atgcgtattg
    12181 gaacggccca ctcaaagggc ttgccgatct gcctgccggg tacaacgccg accccaacga
    12241 caagagcacc atgagcttcc gagacaactt caccggcttc tggaatgtgt ggaagtacgg
    12301 caaggtctcc agaaactacg atctgctcga cgaaatccac cccaaccgga tcagaagtgc
    12361 tgagcaatgg ttcaggcggg aagatcagct gggacgagag ctcggcaagg ggactctctg
    12421 ggacagggtc cagcccgaga accagaccat cgactcggcg atcctcaagg gtagtgcgga
    12481 tttgaggaca ggcacgttgt agacgcatga gcggcaatcc aagcgccgct tgttctcgcc
    12541 gtcgaccacg aatcaggcca aaccgaacgt catgacgttc cttatcgcca acgccacagc
    12601 cgccgctcca tctgtgggtt ccctgtgggt tcctaaattc ggcgcggcat tctcacttgc
    12661 cgtgtcggac actcgcccag tgttgtgggg actcgtcccg tgtcgtaccg tcaacctagt
    12721 tgattagcca tcctagagga atttccctac cgtccgcgcc tgtcgccgct catgaaaggg
    12781 tcacggatgc aacgggtttc gatcagccgt cgactcggcc agcgctggat gctcctggtt
    12841 gcggtgatcg tggtcgcact ggcggggttc gctgtgtatc ggctgcacgg catcttcgcc
    12901 tctcacgacg tcacgtccac acccagcggc tccgacaacg acatcgttcc gttcaacccc
    12961 aagcacgtgg ttctggaggt tttcggcccg ccggggacgg tggcgaccat cacctacctc
    13021 gacgtgaatg cccaaccgca gcgcgtcgac gccgccacgc tgccctgggc ctacgacacc
    13081 aacaccactc agccggccgt cttcgttaac gtctccgccc agggtgaccg agactccatc
    13141 ggttgccgga tcaagatcga cgacgtggtc aaggacgagc gatcggtgaa cactctgaac
    13201 cccttcacct actgcctgga taagtccgga tgagcaccca gcgggttccg gacttcatcc
    13261 ggcggtactc ggtgctcatc gcgctgttct ggctgggcct ggcggtgctg acgaacgttt
    13321 tcgtgccgca gctggagacg gttgccgaat cgcataacgt ctcgctgagc ccccaggatt
    13381 cgccgtcgtt gcaggccgca aaacggatcg gcaaggtgtt caacgagttc gattcagaca
    13441 gttcggcgat gatcgtcctc gagggtgacc agccgctcgg cgccgacgct caccattact
    13501 acgacggctt gattcagaag ctccgtcagg acaccaagca cgtccagcac attcaggact
    13561 tctggggcga tccgctgacc gccgccggct cgcagagctc cgacggcaag gcggcgctgg
    13621 tgcagctgta cctagcaggt aaccagggcg aatcgctagc caatgagtcc gtcgattcgg
    13681 tgcgtgacat cgtcaatcac acaccgccgc caccgggtat caaggcctac gtcaccggcg
    13741 ccgctcccct ggtcaccgat cagttcgagg taggccgcca cggcaccctg aaaaccaccc
    13801 tgatcaccat cggggtgatc gccttgatgc tgttctcgct ttaccgccgc ctcaccacgg
    13861 tgtttctcgt gattttcacg gtgatgatcg agttgaccgc ctcccgcggc gtcgtcgccg
    13921 tgctcgcgaa cgccggcatc atccagctgt cgacgtattc gaccaatctg ctgacgctgc
    13981 tggtcatcgc cgcgggaacc gactatgcga tcttcattct cgggcgattt cacgaagagc
    14041 gttacaccgg gcaggatcgc gtctcggcgt tcaacacgat gtaccacggc accgcgcaca
    14101 tcatcctggg ttcaggcctg accatcgccg gcgcggtgct ctgcctgacg ttcacccggc
    14161 tgccgtattt caacagcctt ggcgttcccg ccggtatcgg cgtctccgtg gcagtggtgg
    14221 cggcgttgac cctggcaccg gccttattgg tcatcggccg ccatttcggg ctgttcgaac
    14281 ccagccgccc catgagtacc cggggctggc ggcgtatcgg cacggccatc gtccgctggc
    14341 ccgttcccat cctcttggcg acggtcgcca ttgccgcgat cggtctgctc tccctgccgg
    14401 gatacaagac gagttacgac gcccgcccct tcatgcccgc cagcgcgcct gcgaatgttg
    14461 gttatgcggc cgccgaacgc catttctcgg aggcgcggct gaatcccgaa ctgttgatga
    14521 tcgaagccga ccacgatcta cgtaattcca ccgacatgat cctgctggag cgcgtcgcca
    14581 aggcggtctt ccacaccgac ggcatcgcac aggtgcagtc gatcacccgg ccgctcggca
    14641 cgcccctgga ccacacgtcg ataccgttcc agatcagcgc gggcagcgcg tctcagatca
    14701 acaatctgcc atttcagcag gcccgcggcg ccgacctgct caaacaggtc gacgtgatca
    14761 acaattcgat cgacgttctg cgacagcaat atgcgctgca acaacagtcc agcgccgcca
    14821 ccgacgagca gaccaaggca ttccaacaga cggtcgccac ggcgcaggac ctgcgcgaca
    14881 agatcgccaa cttcgacgac ttcttccggc cgctgcgcaa ctacttctat tgggagccac
    14941 actgtttcga catcccgatc tgctgggcgc tgcgatccct gttcgacgca ctcgacggca
    15001 ttgacgcgct gaccgttcag ctgagcaacg tctcgggcag catcgccaag ctggacgcgc
    15061 tgcagccgaa gctgctcgcg ctgattccgc cacagatcga aagtcagcag accaaccgtg
    15121 atttgaccct gacgaactac gccaccacat caggcatcga cgaccagact gaggcggcac
    15181 tgcagaacgc caccgctttg ggccaggcgt acgacgcgtc gaagaccgac gactccttct
    15241 accttccgcc ggaggcgttc gccaaccctg aattccttcg cgggctaaag cttttcctgt
    15301 cgccggacgg caaggccgcg cggatgatca tcacccacga gggcattccc gctacgcccg
    15361 aaggcatttc gcacatcgac cccatcaggc atgcagcgca ggaagccgtc aagggcaccc
    15421 ccctggccgg atccaagatc tacatcgcgg gcacggcagc cacctacaag gacattcagg
    15481 acggcgccaa atacgacctg atgatcgcgg ggatcgccgc gctctgcttg attttgctcg
    15541 tcatgatgtt catcacccga agcatcgtcg ccgccatcgt catcgtgggt accgtggcgc
    15601 tgtcgcttgg cgcatctttc gggctgtcca tactgatatg ggaggacctt ttcggtatcc
    15661 cactgttctg gatcgtgctg gcgctgggca tcattctgct gttggccgta gggtcggact
    15721 acaacttgct gctgatctca cggttcaaag aagagatcca tgcgggcatc aacaccggca
    15781 tcatccgggc gatggccggc tccggcgcgg tggtgacggc cgccgggctg gtgttcgcgg
    15841 ccaccatggc atcgttcatt ttcgccgatc tgcggattct cgggcagatc gggaccacaa
    15901 tcgccctcgg tctgttgttc gacacgctga tcgtcagatc gttcatgacg ccgtcgatcg
    15961 cagcgctgct cggacgctgg ttctggtggc cgctgaaggt gcgtcaacgc ccggtacccc
    16021 aggcgtttgc cttacccgac caacgggagc gacaactacc cctgtgggac gacggagact
    16081 ccgcaccgac gtccgagctg ccccgacgcg gctaggcggt ggcgagctcg tcgtccggct
    16141 tgggcgccgt caccgatacg tcccgcagct tccctttcaa ccccgcgacc ctgcgcagct
    16201 gagcgacgat gttcatggag gtcaacatcg ggatcccccc gatgaaggtc ctaaaggagg
    16261 gattgccgcc atctctgtgc agcatgtaga gacagccgac cacgtaaaag aatccgatgg
    16321 tgaacagggc ggcgggtatc gcgtacgcga gcggtgatcg ggcgtcggaa accagttccc
    16381 tcgcgtaatc cacttcatcc tgcgacatcg gttcgcgctt gcggactttg gccagcaccg
    16441 ccttgtgggc gcccacttgt tcacccaagg gatgggatgt ccgcttctcc agccggccga
    16501 tgtaggtgaa tacgcaagtg gcgaacacga tctccatcac ggcagcgagg atcgtgagat
    16561 caccccacgg accgagattc accctgtcgc tcctcttttc cccgtcgacc ctcgcggcat
    16621 gcctgaagtg cgagagcccg cgtcgtcagt gttcgcgcga ctcttgaaac gctacctgaa
    16681 ctaagcattt ctgtcggttc tcacaggaac aagacaggag taggagtgaa gccgctcctc
    16741 gacgtcgtag ccgccgaaac ctccccatcg gccgcgggca acgcgtagtc gcgaatcgag
    16801 attgacgagc tctttcgaca cgccgaagtg atgtcaaaat agcccgccgt tttaggtcaa
    16861 tgtccgctgc aactcgccag ctaaaccccg acgtagctgt aggtgtagat cctcacaatc
    16921 gatgtcatat tctcaccagt catgtcaggt gtcgttctcg ccgccatcga tcgttggtgt
    16981 tcaccgtttc tcattccacc ggtgcggatc gacttcgagc gcggatcggt cccatctgcc
    17041 gcgttcagcg gccgcccgat aggtggtgtg caaaaactcc agaagggtgc ggtctgggtc
    17101 ggctgccgtc cgcaccgcct cgtagggcag cagaaactgg ccattgtcgg tgctgtagaa
    17161 ggcttgctcg ggtacgacgg ggtagtcggc gaagccgtcg ggttcgggat aggcgtaagc
    17221 gtagaaggcg ccttcgtcgc cgccgcccgg ccagaaaccg caactgctca actcgtggga
    17281 gtagccctcc accatcaccc aatcaccgca gttgggtgcc ccgcctggat gcttcggcgc
    17341 ggcgcggccg gagaacctgg tgcaggccag atccattgcc ccccaaaaga agtgcacggg
    17401 gctgaccttg ccgatgaaat gcgagcggaa ctcgcgcatg acgcggtcgg ccgacacgag
    17461 ttggcgccag aacaggtgca cggcgtgtgg gtcgtaggag gcgtgcgtgt ggtcctcggc
    17521 gaacgggatc gcgggatcga cctcattggg cctcgcctga atctgcaccg cgatgccgcg
    17581 ctcgtcgagc gcactgatcg tttcggcata gaaatcggca accgacctgg gttcgagcgc
    17641 gatccgacgg gcgccgccgt cgctggatcg gatgtgcaac cggtgttcga tgaagtcgaa
    17701 ctcgatgtcg aagatgcccg attcgtacgg aatcgcggat gtggtcaaac ctcggggact
    17761 gacgtagagg gtgacctgcc accagtgatt gagcatcggc gcgtgtgcga gtcggatctt
    17821 gccgacgatc tgggtccaca tgtgcagcgt gtcacgggag gcggtccact cgtcgacccg
    17881 caatgacggc caattctgcg ccgacgcggt cttgttcggg gtggtctcag tcatcagaca
    17941 acccttcctc cttgtaactg cgcgggcagt cgcccgccgg ttgtttggag cgacagcagc
    18001 gacgactcat ccggacctcg agcgccatat tgcacgcggt cagcccaacg caggctcgtt
    18061 cacggccaat cgctgggcaa gggccgtgcc cgagccgatg gcttgtccgt caccaaggcg
    18121 accctgccgg caccggtcag tagtcccaga cggccggtac gccacgaaag gcgttgccgg
    18181 cgaccgccac gtggcagacg gacgggaacg gcagatgagt ggccaccagc gcttcacggg
    18241 tcgacgccag ttcctgcaag agcttgaccc ggacgcgggc cgcctcctca gggtcgtgtt
    18301 cgaagccgtt gaaccagtcg gggtgttcga acgcgacctg gaacactgcg tcaccagcga
    18361 acgtcagccg ctcgccgccg gacgccaggc ggaccacgct gtgcccgggg gtgtgcccac
    18421 cggtgcggct ggcaagcaca cccggtgcca cctcgtactc cgtctcgaac ggccgcagcc
    18481 ggccgcggtg ctcgtccagg aaccgcgtgg cggccgatcg aagcgcgtcc ggcaccccct
    18541 ccggcatgtt ggtatgggtg aaatcgggcg actcccagaa ctcggcctcg gcggcggcca
    18601 cgtggacccg caggtccgga cgcagccggc tccccagccc gtcgccaagc agcccgccga
    18661 tgtggtccat gtgcatgtgg gtgagcacca cgtcggtcac ggatgcggag tcgatgccgg
    18721 cggcctccag tcgcagggcc agccgcccgg cagtcgggaa gtccgggaac tcccccccta
    18781 gcccagcgtc gatgagtatg gtccgatcgc cgctacgcac cacgcacacg ttcagcggcc
    18841 actcgagtac gtccggcggc aggaacatgt catcgagcca ggacgccagg tcggccgagt
    18901 cagcgttgta ggcgatcgtc ttgcctgtga ttggtaggac cccgtcgctg atcaccagca
    18961 cctcgatctc gccgacgtgc agcgcgtagc gcgacggtac caactcgtcg agcccctctc
    19021 cgccgaggtg ggaaatgttg tccacgctca tttttgtctc ctcgattctt gaaaacggca
    19081 cgaccgcaac agaagaagtg gccgcctgta tttgtttggc tgcgaacgtc acgccctcgg
    19141 cacgcccacg tggcggttcg cccagtcgag cgcgtagtcg gccacggctt cccagccggg
    19201 agcgccggcc gtgaagtggg gtcgtcccgc gaactccttg tagtccacga cggccttcga
    19261 cttgccgagg agcttcgcgg cctctctgct caccgaggcc ggcaccgtgt ggtcccgttc
    19321 gttgccgagc acgagcagag gcgggcggtc gtccttgtgg aggtcgacct tggtgacggc
    19381 gtgcggattg aagttggcga accccgcctg gaagagcacc cgcccgggac cgggcacgta
    19441 gtagcgctgg taggcagccg cggcgtccgc gtcgttcatg gtgttggtga acctgaagtg
    19501 gaactgcttg ggcgacaacg ggacggtctt catcctgttc gccgggttgc gtagcaccgg
    19561 ccatacggtg cgaatctgcg ccggtggcag acgcagcacg cccttgatct gcgccggcga
    19621 gacggcaacc gcggctgcac cgagaccgcg gtcgagcagc agttcggtga tgaggccgcc
    19681 gaacgaatgc cccatgatca ccggcggggt gtctaggccg cggatgatcc ggtcgtagtg
    19741 gtcgacgacc tcggtgatgc cgagaccgtt catgacagac gggtcgcgac ggagggcctc
    19801 gacctcgccc tccatgcggg gccaggccgg cgcgaggacc tcgtgtccgc gcttctcgaa
    19861 acgcgccttc cagccctccc agctgcgcgg ggtgagccag aggccgtgga tgaggacgat
    19921 gggtgaattc atgattggac tcctcggagg tcgtgaggga gcgtcatacg tcgcactgga
    19981 tgaggatcgc gcgtcaatgc ggtcgctcgc gcccttgact gtgcgtggtc cgtgccgtgg
    20041 tttgcgtagt ttcgaagtgc aacagtggcg acatgccgcc caccacgcga ctcgaagcag
    20101 aatgacaagc ccgaagcccg gcggacgcgg ctacccacca atgtgttgac tacggacttc
    20161 caagggttcg ttggacgacc gtcgacgcga aactgggttt caccaaacag cgcatccgga
    20221 cgggcggcgc gaaccaacag gacctgttgg gcgctcgcag cccgaaggtg tatccataac
    20281 cagggcggag gctgatcccg tgccagagat tctgaccaaa cacgccgtcg tcgacgacgt
    20341 gctggacacc ttccgagaaa tgttgggtcc tgacgccgtc cgctaccgaa accacgtgta
    20401 ccgcggtctg aattatcaac gaaagctgtt gcagttgaac acgattccag acgagatcgc
    20461 cttcgcgtgg gccctgcacg acatcggagt ctggacttcg gggtgggatt acatccgacc
    20521 ctcgttagat caagttgacg aacttgcgcc ccacaaccgc atcgcacacg tcgaccgggt
    20581 gcgtcagatg gtggaatggc atcacaaggt gcgtcgctgc cgagacgact gggtggtcga
    20641 gacattccgg gtagccgacc ggatcgacgt cacgagagga ctactccgca gcgcactgac
    20701 acagtcggat atcaggcaag tggtgaacgc gttcccgtac aacggctttc acgcactgct
    20761 ggtccgcagt gaagcgggct gggctgcaaa gcatccactg aggccattcc ccatgcttcg
    20821 ttggtagtgg gggctgccca agctagttca gcccccagag cggatgaccg tcggcggata
    20881 ccgccatccc aagcgagcag gggccaaagc gcacacgcgg tatcaggtca gcatgtcgac
    20941 tacacagagg attatcggag gcggcgctcg gcggccgtcg ggacacagat ttcaaggccg
    21001 tggcggcagg gtcgttttcg tccctggggt cgggggtgac aaccgtcctt ctgggtgcgc
    21061 gtcaaactac tgcgggcgct ggtcggtagg ggaataatcc gacatgcgac gaggaagtct
    21121 cccggcatcg atcgtggata ggtgatcacg aacgatcggg gagggaaagc ggcaacgtga
    21181 ctgatcgaat ctcgatcgtc atcgcagagg actcattgct ggtccgggac agcgtttccc
    21241 gggcgctttc gaccgacccc gatgtgactg tcgtcggcgt cgccgaggac tactactcgg
    21301 cggtcgaact cgtcgaagag catcggccca cgattctggt gaccgacgtt cgcatgccac
    21361 ccacgtcgac cgatgaaggt attcggctgg cgcggtggac gcggacagcg catccggacg
    21421 tcggggtgat cgtgttgtcg aattacgccg acccgagtta cgcggcgggg ttgctggagg
    21481 gcggaacgac gggccggggg tacctgctga aggaacgcgt ggcgcgtttc gacgaactct
    21541 ccgatgcggt gcaccaggtg gcgaacggcg ggacggtact cgatccgctc gtggtggagg
    21601 cgctgcttgc tcagccccgc cccgcggcca cgctgaaccg gttgaccccc agagaacgcg
    21661 aggtgcttgg cgaactggcg accgggttca gcaatcgaac ggtcgcgcgt cggctggtgt
    21721 tgtcccagcg ggcggtggag aagcacatca attcgatctt cgccaaactc gaactgaccc
    21781 ccgaccagtc agtcgaccgc cgagtcaagg ccgtgttgat gttcctcgac gacagcggat
    21841 cggtctgatg ttgtgcggcc cgcgatcggc aatggtggtg cgtacaccat cgtttttggc
    21901 aaatcgatcc gcgacgatgg cggggtggcg gacctcgtgc gtgtctgggt cgtcgacgac
    21961 caagcaagct tccgacgggc taccgccgcc acgttggatg cgatggacga cttcgtgatg
    22021 gccggcgaat gcgagaccgg tgaaaccgcg atcgagcaga ttcccaacgg cgacgcggac
    22081 atcgtgttga tggacatcca catgccgggg atggggggaa tcgaagccgc gcggcacatc
    22141 tcggctgcac gagcggacgt gatgatcgtc ctgatgtcca cctacgacgt cgaggacctt
    22201 cccgtttcgg cctcggtctg cggtgccgcg tcgtatctgc acaaggaacg cctgtctcca
    22261 gatttgctga ggcggatatg gcgagcgggc gcctgaggct cacgtcggcg gtcggatcgg
    22321 cgccccaact gcgtgcgccg agaacgctcg cggtcgcggc gcttcgctcg ccatcgttgg
    22381 gccacgacct gatgcaccgc ttccgggccg aacgcttcga gttgttcctc tcgggccacc
    22441 tcgcattgtg ggcgagcgcc gggatcctgc tgccgctgtg ggagtacttc tggttgctgc
    22501 gatcgccgcg gttcctggcc ttcgtcgccg tcggtgcggc gcagtgcgtc gtgctcgccg
    22561 tcgggctgcg actggcccgg cgagatcggt atcagccatc gatcacgctc gtctgcatcg
    22621 gcacgtgggt cagcgtttcg ctggtcacgc tcatctctcc gcccctgctt ccggcgatgg
    22681 tcttggtggc gatggtgccc gtcaccttcg ccgagcccta catcagcctg cagcgcggac
    22741 tggtgttcac cgtgatcacc gcgggctgcg tcctggccat ggctgcgctc gcccgcatca
    22801 cccacctgcc agccgcggta gtccaagaga ctcgttggat agagaccgcg ttcatcgtca
    22861 tcgcggtgcc gctcaacgtc cttcacgtca tggtgatcgt ctggaacaac gcctcggcgt
    22921 tgaggacgtc cgagcgaaag ctcgccgaac acgcggccga gctcgccgca tcgcgcaccc
    22981 ggttgatcac cgcggccgac gaggaacggc gccgcctcga acgcgatctg catgacggcg
    23041 cccaacaaca cctcgtcgcg ctgtcggtgc tcgtccagtt ggcgcgcaac gccgaacgtg
    23101 accgcgccgc gccgttgctg accgaggcgt ccgagctggt ggatacggcg atctccgaga
    23161 ttcgtcggct cgcccacggg atctatcccc cgctcctgat cagcggaggt ttggcggagg
    23221 cgttgcctgc gctggccgcg cgggcgtccg tccccgtgca actagacctg gggcggattg
    23281 accgctaccc gccttcgacc gaggccgcgt tctactactg ctgcagcgaa gcgctgcaga
    23341 atgccgccaa acacggcggc ccggacacca ccgtcacggt caccgccgtc gccgacgaac
    23401 agacgctcac cttgacgatc agcgacaccg gctgcggatt cacccccgcg atcatgggga
    23461 tggggctgac gaacatgacg gaccgccttt cggccattgg cggtcggctc gggatcgaca
    23521 cggcgcctgg acgtggtact cgcatcaccg ccgccgtcga cactgaggtg gagccggcca
    23581 gctaggccat accacggggg tgtgcacacc accaccaacc gtgttgcgca cacggtggtc
    23641 agggcggtgc ccgtcgcaga aggatagggc cacaccttcg aaagtcattg tttgacagca
    23701 tcttcaagag cggaggctcg tgtgtcgaca gtctcaagtc cagcgggaca acgctccacg
    23761 gcccgcgccc agatgttgtc gcggctggcc catagtggcc gagatgtcat gtgctacacc
    23821 gtcggcgatg agcgaaacat cctgctcaat caccccgacc atgtcgaaca cgtgcttgcc
    23881 gcgaaccgcg ccaactacag caaggacacc gcggccaacc gctacttccg caccgagatc
    23941 gcagacggga tcctgaccgc cgacggcgac ggctggcggc gtcagcggac cctgctcaac
    24001 ccggtcttcc gcgaccgggc acggctggcg accgctgcac gcgaagccat cgccgccctc
    24061 gtcgcgcggc tcgacaagct cgccgacacg ggccgcgagc tgaacctgtc cgccgcgatt
    24121 ggcgaagtga cactgtcgat tacggtccgc gcgctgttcg ggatgagcca cgaaccgttc
    24181 atcgaccagt gcattgcgct gggtggcgca ctggacgatg cgaactccct gttgccaggt
    24241 atggacgggg cgggtccgcg agatgggctc taccggcaac tccgcgcagc gctgtccgcg
    24301 tcggcccgcg gcgagtacgg gccggccctg gccgcgctgg ccgacgaccc cgatcactcc
    24361 ggcgaggcgc tctaccaaca gctggtgacg ctgctcctgg ccggattcga gacgacggcg
    24421 aattcgctca cgtgggcgtg gatcctgttg atgcaacatc ccgacgtcta cgaggagtgg
    24481 cagcacagcc ttgggacgtc ggcggcgctc accaaggcct tgttcgacga aaccctgcgc
    24541 ctgtacccgt cggcctggat cctcggacgg cgcgcgctca gcgacgatca cgtgggcggc
    24601 gtcgacatcc cggccggagc cgccgtaacg atcagtccct ttctgctgca tcggcatccg
    24661 aagttctggg acgacgccga agcgttccgt ccgcaccgct tccttccggg cggcgcgcgg
    24721 cccgctcacc gttacgccta catcccgttc ggcgccggac accgctactg catcggagcc
    24781 acctacgccc acgacgaggc cgagatgatc ctcgacgagc tcggccgacg gttcaccttc
    24841 cgatcggaac acgtcgccga cgcccggccc gagcacaagt tcgtgctacg cgcccctgac
    24901 ccgctgcgcg tcaccgtcca ccggaggaat acgccatgac cattgcgccc gcatcgccaa
    24961 ccgagttcgg cgcggcagtc tgtgccctga tcgcagagca cacgaacctg ccagtggact
    25021 tcatcacacc cgaccgtgcc ttcgacgacc tccccctcga ttccctcggg atgctcgaac
    25081 tcatcctgct cgccgaggcc accttcggga tcgtcgcctt ggatgacgac gggatcgaag
    25141 tgcacaccgt cggtgacgcg atcacattcc tcgcatcgaa attgccagtc gcacagaacg
    25201 attaggacac aacgcatgag cagcctcacc gatcagatgt acgccaacgc ggcgaccagc
    25261 ccgcgcggga tgatcaccgg aaccttcgag gacccgatcc ggcgcagttg gggcgaggtg
    25321 cacgacgaag cgcgacggat ggcgggcgca ctgtcgaaga tgggtgtcga gccgggtgcc
    25381 tcggtcgcgg tgctggccgg cgatccagcc gacgtcgccc cgctcgctca ggcggtctgg
    25441 atgcgaggcg catcgctgac catgcttgcg cagcccacgc cgcgcaccga cctcgccatg
    25501 tggttggccg acacgcatcg cgccgcacgc accgtacggg cagccctgac catcgtgggc
    25561 gcaccgttca ccgaggcagc cgaacctctg aatgcggccg gacaagacgt ggtcatgctc
    25621 gaggagcttc ggactggacc cgccatcgaa ccgctgcatc ccgacgagtc ggcgatcgcg
    25681 atgtaccagc tgacgtcggg gtccaccgga acccccaagg ctgtccagct cacccacgca
    25741 aacatcgtcg ccaactcgaa cgccctgttc acggcgacgg acggcgatgc ggccaccgac
    25801 gtgatggtca gctggctgcc gctgtcgcac gacatgggca tgctcgcgtt cctcgcgctg
    25861 ccgatgcagt acggcgtcga gctcgtcgcc tccaggcccg aggagttcct gcgccggccg
    25921 gcgctgtggg cggacctgat cagccgctac cgcggaacca tcacctcagg accgaacttc
    25981 gcgtacgcgc tgctcgccct gaccctcgaa cgggccgacc ccggccgctt cgatctgtcc
    26041 agcctgcgcg tggcgatcaa cggcgccgaa cccatcgacg cccgcgacac ccgtcgcctg
    26101 gccgaggcgg gtgcacgatt cggactgcgt cccggggcga tcaccccggc gtacgggatg
    26161 gccgaggcca cgctggcgat caccttcgac aactcaccac aggtcaacgt ggaccggatc
    26221 agtcgcatcg atctgcagga acgcgcgctg gccacgccgg acgccggagc cgacgcgtgc
    26281 ggggtggtct cggtgggtct tccggtggcg ggcatggaga tccgcgtcgt cgactactac
    26341 tcgcggccgc gtccgccgcg acacgtcggg tcgatcgagg tccgcggcgc agcggtgtcc
    26401 ggcggatacc tcgccgacca ggggtggcgc gagggggcct tcatccgtga cggctggctg
    26461 cacaccggcg acctcggcta cttcgacgaa cacggcaggt tgtacgtctg cggccggacc
    26521 aaggacgtga tcgtcatcgc gggcaacaac atctacccga ccgatgtcga acgcgccgcc
    26581 tgcgccgtgc ccggcgtccg ggccggcaac gcggtggccg tccgcatcga cgccggcgag
    26641 ttccgggagg ggttcgccgt gctcgctgag tcgaagctcg cggccgatcc cgttgaggcg
    26701 gagcgcattc gcgccgagat cgcccttcgt gtcaaccagg ccgtgggacc caacgcacga
    26761 gcggtactgg tgctgccgcc gggcagtgtc cccaagacgg ggtcgggcaa gctgcaacgc
    26821 cgcaaggccg ccgaattgtt tgcgcgcctc gacgccgagg cggtgccggc atgagcccga
    26881 aggcgacgac gagtgaggat cgcagcgagg aacgagcgag gaccggagcg aggaggagtc
    26941 gaggcattag caaagccccg gccatcgagc tcgagcggct gcggatcgtc cgcggcggca
    27001 acaccgtcat agacgacgtc accgtgtccg tctcggaggg catcatcacc gggttgctcg
    27061 gcccgtccgg ctgcggcaag accaccctga tgcgcaccat cgtcgggaac cagaagatca
    27121 ccgcggggtc ggcgacggtg ctcggccacg ctgccggtgc caagagcctg cgtcggcatg
    27181 tcggctacgt gacccagtcg ccgagcatct accgagacct caccaccatc gagaacgtcc
    27241 gctacttcgc ggctctgtac ggcatcggca tgcagcgcgt gcacgaggtc gtcgaggccg
    27301 tcggactgac cgggcagaag accgatctgg ccggcaacct ctccggcgga cagcagggcc
    27361 gggtgtcgct cgcgtgcgca ctcgtctgtg acccggacct gctcatcctc gacgagccga
    27421 cggtggggct cgacccgctg ctgcgcgtcg aactgtggga acagttcgtc gacatggccg
    27481 accgcggaaa gacgttgctc atctcgagcc acgccatgga cgaggcgcgg caatgcgccg
    27541 aactgatcct gatgcgcgac ggcgccatcc tcgcgcacgc cgagcccgaa gcgattctgc
    27601 gccaaaccaa ttgccttgac ctggaatcgg ccttcgtggc actcgtccga tccgagctcc
    27661 agcccgtcga ggaggtccgg tcatgaaccc gaccacccac tgggcgacga ccgtccgaat
    27721 cctgactcag ctgcgtcgcg accggcgcgc gatgttcatg atcctggtga taccggcgct
    27781 gatcaccgtc ctgttctact ccacgctgtc gttgcagggg cccgggcccg acggcttgac
    27841 accgtatgac cggcttggca tcttccagat cgccgtcatc ccctgcgcgg tgatgttccc
    27901 catcgccgcc atcacgatgc gccgcgaacg cgcaagcggc acgctggacc ggctgttcac
    27961 caccccaatg tccaagttgg acctgctggc cggatacggc accgcgttct cgctcggcgc
    28021 cgtcgcgcag gcagtgatcg tcacgctcgt cgcctaccgg gtgacggacc tgagcacggc
    28081 cggcagccca ctgctcgtgg cgatggtctg cgtgctgacc gccgtgctcg gagtcgccct
    28141 cgggttgttt ttcagtgcgt tcgccgaaac cgagttccag gccgtgcaat tcgcgccgat
    28201 cgtgctggtg ccccagctgt tcctggccgg agtgttcatt cctcgcgacg aaatgcccgg
    28261 ttggctgcat tcgttcagcg acgtcctccc gctcacctat gtcgtcgagg cgttgcagca
    28321 gatcagtgcc aacgcagagg cgaccccccg catgtggcgc gacgtcggtg tcgttgtcgc
    28381 cttcatcatc gccgcggtcc tcggcggcgc cgcgactctg cgccgacagg aagcctgagc
    28441 tatgtacacc ttcgcccacg acggcgtcac gttcaccgtc cagcccgccg agcagggcag
    28501 gcacaccgtg gtgttcgtca acgggctgtt cggcggcgga tggatatggg aacccgtcgt
    28561 cgccacgctg accgctcagg ggtacgggac cgtcgtcacc gccgaacccc tggctgcaca
    28621 cgagaccgca gaagacattc ccgcactgct gcagagcatc tcgttgctgg tcgacacgct
    28681 gcccgatgcg gccccgatcc tctgcgggaa ctccctcggc gcgctggtgg cgatggagtt
    28741 ggcggccgcc gagccgtcgc ggtgggccgg cgtgatcctc agcggagcac ctgggctggg
    28801 tgacgagcag gacgcggaca gcttcggttc cgcgctgcgc accccgtcac tgcggctcgg
    28861 agacctcatc gccgaccggc tgattcacga caaggaactg atcacacccg aactggtcga
    28921 gcgctgcacc caggcgttga cgccacgcat catgcttcgc gccgggcggg cgttgcgcgc
    28981 cacgcgcggg tatgacgcgc gagggctgtt gggccgcatc gattctcccg tgctgctgat
    29041 ctgcggcgcc tgcgacgaga tcagctcgcc cgcgaaatgg cgagacgctg ccccgatgtt
    29101 ccccgacgcc gactacgtcg agatcgcagg agcaggccac tcgccgatgc tcgagaagcc
    29161 ggacgagttc tcggccctcg tgactgcctg gctcgacacc attcctgcgc cgcacctctc
    29221 gggtaccggc taaccgcaac agctttcacg aaggaaccga ccatggattg cacgctttcc
    29281 gcctacgaca ctctcgtcga ttggctgatc gacaggatcg ccgtctacct cgccaccagc
    29341 ccagctgcga tcgagaccga taagacgttc ggagactacg gcctggactc ggccttcgcg
    29401 ctcaatctgt gcaccgacct ggaaaccgaa ctcggaatcc tcgtcgaacc caccatcgcg
    29461 tgggactacc ccaccatcgg tgacatcgcc ggtcatctcg ccgaaagcac cgggaggacc
    29521 cgatgactcc gatcgcaagc cagattgctg tcgtcggcat ggactgccga ttcccctgtg
    29581 ccacagacat cgacgagatg tggcggctgc taatggccgg agaggtcgcc aagagtgtgg
    29641 tgcccgccga ccggtgggac atcgaccgct tccacagcac acagatacgg ccgggcacca
    29701 tgaacacccg gtacgcccac ttcatcgacg acgccgacct gttcgatcac gaattcttcg
    29761 gtatctcacc gctcgaagcg gccgccctcg acccgcagca acgcctggtc ctgcaatccg
    29821 tttggcgcgc attggaagac gcagccatcg acccgcgcgc acttgccggc accgaggcag
    29881 gtgtgttcgt gggcatgatg ctgagcgagt ggggcgcgct caatctgttg gactttccat
    29941 cgctgacgtc gcagcgcgga atcggcggtg gacactcgat ggtcgcaaac cgggtgtcgt
    30001 accacctcaa cctcaccggg ccgagcgtca cggtcgacac ggcgtgctca tcgtctctta
    30061 cggccgtaca cctcggctgc caggctttgg cctcaggcga caccgacctc gtcgtggcct
    30121 caggtgtgaa cattctgctg acccctgcac tgtccatctt ctatacccag gccgggctgt
    30181 ccgcaccgga cggcacctgt aaaccgttca gcgccaacgc cgacggcatc ggccgcggag
    30241 agggtgtcgg cactgtggtg ctgcggcggc tcgacgacgc ggtcgccgac cgccagccca
    30301 tctattcggt catcgccggc agtgccacca accaggacgg caagagcagc ggtatcaccg
    30361 cgccgaaccg ctgggctcag tccaaggtga tgcagcgcgc actcgatcgc gcgggcgcgg
    30421 ccgccagcga tgtcacgttc gtcgaagccc atggcacggg tacgacgttg ggcgacatga
    30481 tcgaagcgaa cgcgctcggc gacctgcacc gcaccggccg cggcgccccg tgcctcctcg
    30541 gctcaatcaa gggcaacatc gggcacgccg aaggggcggc aggcatcgcg tcgctgatca
    30601 agaccagcct cgcgctgtcg agggggttgc ttcccccgac cgttgcggca gccggaccca
    30661 acccggccct acggctggac cagcagggcc tgacgctggc caccacggca gccgagctgg
    30721 gcgatggccc ggtgctcgca ggtgtcagct cgttcgggat cggcggcagc aacgtgcacg
    30781 tgctattgac ctcccctccc gccgtcacgg cggtgcccga ggtccatgac gacgacgagg
    30841 tgggtgtcat caccgtgtcc gcgcactcat cggatgcgtt gcgccgcaac gccactgcgc
    30901 tggccaacgc cctcgatgag aacaacgacg acgccgtcgc cgccttgtgc tacaccagca
    30961 accaagtcaa gtcgtcgctc aaacaccggt tcgctgcagc gggcacccgc aacgagttga
    31021 tcggtgccct gctcggttac gacatgtccg cgcgcaccga gaagtcgcct cgtcgcggcg
    31081 gcaggctgcg gatcggcctg ctgtgcaccg gacagggtgc ccagtacccc gggatgactc
    31141 aggcgctcta ccgcacctgc ccgccgtacc gtgagcatct gacgcgggcc gccgccgccg
    31201 tcgataccac cttcgcggga cctaatggat tgctcaccaa catgtttcgc ggcggcgacg
    31261 tcatcgacac caccgagtac gctcaaccgg cactgttcgc ggtgtcgtat gccatcggcg
    31321 cggcgctact cgacctcggt gtcgagccga cgttcctgat cggccacagc gtgggcgaat
    31381 tcgcggctgc agcgctagct gaggtcttga cgctcgacga agcggccggc ctggtggttg
    31441 cccgtggcgc ggtgatgcag caactgccga agggcggtgc gatgagtgcg gtcgacgtcc
    31501 ccgccgaaca ggttgcgaat cttgttgcag aagagccaac ctgtggaatc gccgctatca
    31561 acggaccggc ctcgacggtg gtgtccgggc ccgccgccgc tgtcgatcgc atcaccacag
    31621 cacttgcccg cggcggcgca aaggtcacgt cactgcccgt ctcgcacgct ttccattcgc
    31681 ccctcatggc gcctgcgcag gacgcgttcc gcgctatcgc cggcgcagtg acaccgcgga
    31741 aggcgacgat cccgatcatc tcgaccgtgc acggcacgat cgtcgacggc accgacatgg
    31801 acgccgacta ctgggccgcc cagctcacgt cgacggtgcg attcatggat gcgatgcgta
    31861 ccgccaccac gcaggcgtcc ccgacccacc tcgtcgaact cggcccgcgc acgaccctgc
    31921 tggcgcatgc gcggcgcggc ggcatcgaca tggacgtggc aaccctcgcg ccctgcgtcg
    31981 ggcccgacga cgacggcgcc ggtttcgcgc gggtcgtcgc ccgtctgtac agcgacgggc
    32041 tcaccaccca gttcgaccat ctctacgatg cggagtcacg gactctgaaa cggttgccgc
    32101 cctatgtctt cggcgacgga tcccggttct ggcgcgtact ggacacagcg gcggtcactg
    32161 gctcggtatc cactgcgacg tcggtcgtgg ctactgaacc aggccccgcg tccgagatag
    32221 ccgacgccgc gccgctcgcc tcgcgtatcc ggcagctcat cgccgacatc ggcggctaca
    32281 ccgtcgagca gatcgatccc aacgcgctcc tcggcgcgga cctgggctac gactccctgc
    32341 tgcagcttcg cctcatcgag tcgatccgca ccgagtatcc agagctgcag gaggcgcctg
    32401 tcagcgaatt gttaagtgcg atagagaatg tcgacgatgt agtgcgctac gtcgccgacc
    32461 ggttggcgga ggtggcccga tgaccatcgt cgatatcgtt gcggcccgcg caacgtcgga
    32521 atcgctggca tacgtctatc tcgcagacgg caccccggcc agtgagctgc gctggtcgtt
    32581 cgcggacacg gcgagccgca cccgcgccta tgccgcgcac ctccgcgatc gaggtatcag
    32641 tgccggacac cgcgtcgtgc tcgcgatcaa tccgagcctc gactacatcg cggccctgta
    32701 cggcatcatg tggctgggcg ccatccccgt cccgtgcttc ccgcccctgc gcacaaagga
    32761 actggaccgc ttcggagcga tcacggtcga ctgcgcgccg acggccatcg tcatcgacga
    32821 gatgtaccgc gagccgatcg aggctctgcg ccgtcgcctc gagtccgtcg gcctggagcc
    32881 gacggtcgtc tatgtcgagc aggtcgacga cgcaggcgat gactccccgc ccgtacccac
    32941 tgctgcgcgt gacatcgcgc tgatccaata cacctccggc tcgaccggga cgccgaaggg
    33001 tgtctgcctc acccacgcca acctggtcag taactgcgag gcgctcggtc gcaacatggg
    33061 tcacgacccg gaccggatcg ggttctcctg gctgccgccc tatcacgaca tgggactgat
    33121 gggcacgatc ctggtgtcga tgttccaggg tgtcccgctg gtcacgatgt cgccgctgca
    33181 cttcgtgcag tcgccccgcc gctggctcga ggccatcacc cagtacaagg tgacgatcgc
    33241 cgtgggaccg aacttctccc tcgacatgtg ctccgatgtg ttggccgacg gcgacaacac
    33301 cgacctggat ctctccacac tcgagcggct gtactgcggg gccgagccga tcagcatcga
    33361 tacgctcgca cggttcgaga aagccgcggc gccgctgggt ttcaacctgg acgccttggt
    33421 gccgtgctac gggatggcgg agacgacgct gtacgtgtcg gggaagagcc ccggccgcaa
    33481 ctatcacacc gagccctcac ccgaaccgtc cgcgctgccg cgggacgtcg tcagctgcgg
    33541 gaaggtcgac cccgagcaca ccgttcggat cgtcgagccc accagtctcg aggctttgag
    33601 cgacggacgc ataggtgaaa tctgggtggc aggcccaagc gtcgcggcgg gctaccacga
    33661 tcggcattac ctgacacgca gggtatttgg cgcgcgaatc gccggcgagc tgggtgggta
    33721 cttgcggacg ggtgatctcg gcttcgtccg atccggtgag ctgttcgtca cggggcgcat
    33781 caaagacctg atcatcgtca acgggcgcaa tatctatccg caggacgtcg aggccaacgt
    33841 tggacgcgca gaccccgccg tccgtatcgc ggtggccttc tcgattcccg gtgacaactc
    33901 cgaacagctg tgtgtcatcg ccgaagtcgg ccagccgcag gtgaccgcac aggtgcacgc
    33961 tcggttggtc gatgcgatac gggccagcgt gacatcggaa ttcggcatcc gaccgcatgt
    34021 ccacatcgga ccgaagcgca cggtgccgac cacgaccagc gggaaggtac gccgtcagga
    34081 gacccggcgg atgttcctgg ccgacgaact actttccgtc ccaatcgatt caccagccac
    34141 acaggagtgc acggcatgag tcagctggca tccacacgac acccggcacc gaacgagcaa
    34201 gcaccgctgc ctgaacgcga cgtcctggca tcgcgcctcg cctacctcac ggtcggcatc
    34261 cccaccttgg gaacggtcgc cgccgtcggc tacgcgatca gcgacgggct gtggctgtcc
    34321 gacgtcctgg tgttccttgc gatgtacctg atcacagggc tgggggtgga ggggggcctg
    34381 caccgctact tcacgcatcg ctcgttcgtg gcgtccgaac cagtgaaggt ggtcctcgcg
    34441 gtcgcaggct cgatggccgc gcagggtccc gtgatcttct gggtggcgaa tcaccggcta
    34501 caccacgcct tcgccgacac cgatcgcgat ccgcattccc cccaaccgca gggaccaggg
    34561 tggtggggcc gggtaaaggg actgtggcac ggccacattg gctggctgtt cacggtgaac
    34621 aagatcgact ggagtcgtca cactcgcgac tggttgcagg accgccgcgt gatgaagatc
    34681 aacgggcact acttcaccgt ggttctggcg ggcatcgtca ttccgggact ggtcggtggc
    34741 cttgccactc aatcgatgca cggcttcgtc ggaggcatcc tctggggcgg cttcgttcgg
    34801 atcttcgcac tcgaccatgc gacctgggtg gtgaactccc tcggccacac aatcggaaac
    34861 cgcgagttcc acatccgcga ccagagccgc aatatcggag ttctcgcgcc acccaccatg
    34921 ggcgggtcgt ggcacaacaa ccatcacgcc cggcccagcc tcgcgcagac ccgccgacac
    34981 tggtggcagc tcgatctcgt gggggatttc atcgggctgc tcgaccgcgt cggactcgtc
    35041 agcaacgtcc gctaccccaa cacgcaagaa gagaaccatg atgatcgaaa cagctagtcc
    35101 aacaacggct tcggattcca gacaagagtc gctggcccgg gcaatcgtgc agttgaaccc
    35161 gaagagcgcg cgcgccaagc gcgtcgtcgc cgcggtcacc gttgggatac ccgcggtcgg
    35221 cttcgccatt gccgtgtacc tgggcgttgc cggcacggcc actgcgttgg actacctact
    35281 cttcgcggtg ttctacgtga ttcagatgtt cggcgtcagc atcgggtttc atcgctacct
    35341 cgcgcacaac tcgttcaaga cctcaccgtt cttcgagggc gtactgatgg tcaccgggtc
    35401 gatggccctc gaaggtccag tcctgttttg ggtgagcacc caccgccgcc accaccgcta
    35461 ctccgacgaa ctgggcgatc cgcactcacc gaacctcagt ggcagcggac tggccgggaa
    35521 gctcaaaggt ctttggtacg cccatattcc gtggatgttc agcgaccagg agtcccgagt
    35581 gaccgtgttc gctcccgatg tggtgcggga tcggcgcctc tacttctaca accggacgta
    35641 tcccgtctgg gcgttgacga gcctgctgct ccccgcgctg ttggggttca cgatcagggg
    35701 cacggccgca gcggcgttct ccggtttcat cttcggtgga ctggcgcggg tgttcgtcgc
    35761 caaccaggcg gcctggtgcg tggggtccgt cagccacatg atcggttcgc gaccgttcgc
    35821 gaccagagat gacagcgcca acaactggcc ggtggccatc ctcacgttcg gcgagggcct
    35881 gcagaacaat caccatgcct tcccgggcgc ctaccgtcac gcaatgcgtt ggtgggaacc
    35941 agatctcagc gggtgggtga tcgccatact cgcgaaggcc ggcatcgtct ggagcctcaa
    36001 gatgccggac cgacagaaga tcaatagccg tctgcgacgc aatcgcccgt cggtgaagtc
    36061 caccgacgaa cttaggaaag aaggttgaac aatgttcgga cgcaatagaa ccgccacgat
    36121 agacggcccg ctcacacacg agaagctacg gtcgtggttg atcgcggagc tggcgcggat
    36181 ggtgaagatc gaccaggcac aggtcgacac cgccaagccg ttccaggcct acggcctgga
    36241 ctcgcgtgcc gccgttcaag tttcgggggc tttggagaag gttgtggagc gtcggctctc
    36301 gccaggaatc ctcttcgacc acgagacgat cgacgagctc gcggatcacc tcgtgggtga
    36361 actgcagctc gagcgcggct gagcacagga gatgcgtcat gacgaaattc gtcaccctca
    36421 ccgatcctga cccacggttc acggagttca tcgagaagag caacaccctc gaaggcgcga
    36481 agctcgctgc cgccatcccg aaggagtacg tgcaaccctc actggcacgc gggctcctcg
    36541 gccttgcggc cagcctgtcg gtgtatgtcg gcgccatcgt cgcgatggcc gttctcgatc
    36601 ggtggtacct ggtagttccc ctggctatct tggcgggtct gggcgggtgg ggcctgcact
    36661 gcatcgcaca cgagtgcggg cacggatcat tctcccgaaa tcgccggttg aacttcgtga
    36721 tcgggcacct gacccttgtg catctgctgt atccgtttca cgcgtggcgt catgttcaca
    36781 acctgcatca cgcccacacc aacagcctgg agctcgacac cgactgggcg cccattcccg
    36841 aggcggtcta ccaccggatg gcgttgcggg accgaatgat ctacatcagt gtgcgcacgt
    36901 gggcggtgtg gggcgggacc atccaccact ggctgcaatc gggtctgcgc ccgtcctact
    36961 tcccgaagcg agagatgcgc cgggacgcgc gccggtcgat ggcgttcgtt gccgtcgtcg
    37021 gaggcgccta cctcgccgcg ttgacgtact tcaccggctg gcaaggtcta ctgctgtact
    37081 tcgtcgcgcc gtggttcgcc acccacgtgt ggttcagtat cacgacactg atgcatcaca
    37141 gcgccgagga catcccatac ctgacgtccg agcactggac gcgcaatgcc agcaagttgc
    37201 tcgtgacgac ggatttcgtg tatccgcggt ggctgctgtt cctcacccac aacatctcgc
    37261 tgcataccgc acatcacgtg tcgcccgccg tgccgttcta ccacctcggc aaggcgcagg
    37321 ctgcgctgct gcaggcgtac cccgagatga tgcgggtgga gaaggcgagt gcgcgcaaac
    37381 tatggaacat cctgcgcaac tgcaggttct acgatccggt ctccgggctg tattcgacgc
    37441 gcccgatgcg tcgggtcgtc ccccagcaga aggaatcagc gtgagcgccg gattgtttgt
    37501 ggcccaggcc ttgacacgcg cacatcggat cgcaccgcgg gctgctgggt acgtctggac
    37561 gagcatcttc accaacaccc gcgggctcgg caccgcgccg aacgacctgt gccccttggg
    37621 cgccagccgc gtggaactga cgggcgtgcc gagggtcgcg gcggtgtatg tgtggggcag
    37681 cgcggagccg acggttcttg cgctgcacgg atggggagcc gacagcacca ccatgtcaag
    37741 cgtggtggct gcggcagccg acagcggcga gtcggcggtg tgcttcgacg cacctgggca
    37801 cggagcctcg ccgggaacac aagcgacgat gagggagtat tcagacgcta ctctcgctgt
    37861 cctgcaacgg tttccgagca tcaccaccat cgtcgcccac tcatttgcgg cgatcgccgc
    37921 agtctcggcc gtcgcagcgg ccgccggcaa cagcgtgcga tttctgctcc tgctcgctcc
    37981 ggcgtgctca ctgtcggacg ttctcgaacg atgggccgct catcgggatc tgccaccggc
    38041 ggtggtcgag cagattcgtc gtgaactacg gcgccgcgac ggcatggagg tatctcattg
    38101 ggatgtcaga acactcggtc ttccggcgtc ggtccgagtt cagatcctgc acgatccggc
    38161 ggacgccttg gtgccagtga gcgactcgta ccggatcgcg gccagcatct cggcggagat
    38221 ccaagcgact atgccaggcg ttggccacca ccgggtcatc tcctgcgacg agatgcgacg
    38281 ggctttgact gcgcgggcga ccgcttccga tcgttgagta tggcaatatc tccgcttgcc
    38341 ggcgcttgct atgttccgta cgcgagtgat ggggaatttc gctctctatc ttgaggggtg
    38401 ggcggctgat ggtgattcag cagcgtgtcg aggtagcgga gccgttcgcc gagccgtcgg
    38461 ctcagctctg gcaccctggt gagggcaggg ggccgagcgc ctcgaggggc tacctgtgcc
    38521 gtcgccatcg gactcggatc agtatgtgac ggttgacgag cgggttgaga acaccattgg
    38581 ggccggcctc ggcgaccctg atcctcggtg gcgatgccca gaccctgtcc tggtcgttcg
    38641 tggcgaccga ctttcctttc ctggccgcgt cgggcagcgc gcaagccaag gcgatctcgg
    38701 ctgccaccgg gaacaaggat ctgttctcgg ccaccgtgct caaggtgtcg catcacgcgt
    38761 cgaagcacgg cgtcaatctg gaactcgtcg aacggatcca ccctgcggtg acgctggtgt
    38821 cgtccaccgc aagcggcacg aagtacgggt tcccccacac cgtgtcgcag gaagccatcc
    38881 gggaggcgct cgatccggtc gccagcaagc tcaatccggc caccgggctg cctaaagacc
    38941 acaagtccga tgccgaactg cgcatctttc tacaccagcg ataccaccac gggaccgggc
    39001 ggccccgatg ctggatcctt cgccctattg ctgcgcggcg agcgtcgcga gctctggcgc
    39061 ttcggcgatg ctcccagcca atcggtcgac ctcagcagtg ccaggctgtg gacgtgaagc
    39121 ccgacccgcg ctgagtccgt cgcccggtca gcggaagcgg acgtccaacg tcgccagacc
    39181 gaagatcttc ttgccgtcgg acttcgccga gacgatgaca accccggagc gggtctcagg
    39241 gtccagcgac ttgatccggc cgccgaactc gatgtccgcg cccccggtgg ccgacacgat
    39301 cgcgggcgcg gacagccgca ccgcgtagcg ggtaaccgca ccgggatcac ccgaccacgc
    39361 ggaggcgaag ccggcaccca accccatggt gagcattccg tgggcgatca catcgggcag
    39421 cccggccagc ttcgcgatgg cctcgtccca gtgaatcggg ttggcgtcgc cggccacgcc
    39481 ggcgtagttc accaggtcgc cgcgggacag tcgggtgtgg tgcaccggta gctcgtcgcc
    39541 gaccttcacg tcatcgaacg acggcgtgcc cggcgtgcgg gcggtgctgt cggcgatccg
    39601 cacctcgccc tcggggcgca ccgtcttgtg gtactcggat tcgccgaccc cgaagatgtc
    39661 gacgtcgtgc atcatcgcgt tgtgcacggc tgtcttgatc tccggattga gatcctcggc
    39721 ggtgacgccg acgacggtgg tgtgcagggt gtgcacccgc tcgccggcgg tgtcggtgaa
    39781 ggtgttggtg acggtgatca ggtctctgcc ggcgatgcgg cgcactgacg tcagttcgac
    39841 gtcgacgtgc agttcgtcgc cggccacgat cgggcggtgc tgctcgaaga cctcttcggt
    39901 ctgcatgtag gtgtcgtaac cgacgaccac ctgttcgaac atgtggcgat tgcaggtcat
    39961 gcctggagtc gacgtaaacg tcagcggcgc catgaggtcc gaatatccca gctcagcggc
    40021 ggcggcgaca tcccagtgcg cggggtgata gtcctgcacg gcacgggcgt actcgcgtac
    40081 cttctcgcgg ccgaccaggt aggtgccgtc catctggtag tagtggccga cccgcgcttc
    40141 aagcagcgat gcttctggtg cggtcatgaa ttcgccctac ttttctatcg gctggttcgc
    40201 tgaggccgac caagcaccct aacgcgccgg catttgtcgc cgggattggc gctcgatggc
    40261 tcgaagcact aattttccgg aggtgatgtc ctcagctgct ggtgcagtcg ctcggtggat
    40321 cgctcccctt ctaaccgttg cagcggtcgc ctgcgtgggt ctagttgccc actcggtgcc
    40381 cgccccggcg gtgcgcctgg cagccgatga gggaaacccg ctggctgggt tgccgttctt
    40441 cgtcaatccc acatcggcgg ccatgcgtgc cgcgcagaag gccgatccgc cgagccccga
    40501 gttgaccgcc atcgcgaaca caccgcaggc gtactggctc gtcccgggca cttccgccgc
    40561 cacggtcgcg aagtacaccg gtgatgcgca ggctgccggc gccatgccgg tgctggcgct
    40621 ctatggaatc ccacaccgcg actgcggcag cttcgccgcg ggaggcttcg cgacaggcgc
    40681 cgagtaccgc cagtggatcg acggcatcgc cgccgacctt ggctcctcgc cggccgcggt
    40741 aatcgtcgaa cccgatgcac tcgccatggc cgactgcctg tcgggtgacc agcgccagga
    40801 acggttcgac ttgattcgct acgccgtcga cacgctgacc cgtaatccgg ccgcggccgt
    40861 ctacatcgac gccggccact tgcgctggca cagtgccgac gaaatggctg ccaggctcaa
    40921 ccaggccggt gtggaccacg cgcgaggctt cagcctcaac accgccaact tcttcaccac
    40981 cgatgacgaa attggttatg gcgaagcgat ttcggggctc acgaacggtt cgcactacgt
    41041 gatcgacacg tcgcgcaacg gcgccgggcc agcgccggac tccccgctcg actggtgcaa
    41101 ccccagcggc cgagcactgg gcaccccgcc caccacggct acagcaggcg cacacgccga
    41161 cgcctacctg tggatcaaac gtcccggcga atccgatgga tcctgcaacc gcggtgatcc
    41221 gccggcgggc aacttcgtga ctcagttcgc catcgctctg gcgagcaacc caggccatta
    41281 aacgctcggt agcgtcgtcg tatgacggac aacccggcag aagtagtcga atcggtcgag
    41341 ccgcgacggt cgatgcggca ctactggcgc cgcatggcga tcgtgctgac catcacgctg
    41401 ctgctgttcg gggtgccgtg gtggacgctg ttcgcgccga gcgcatcatg gccgacggcc
    41461 gtgttcgtgg tcggcacgct gctcttcgtc ggcgcgttcg ctgcaatgcc tctgctgatg
    41521 gtgttcggtc acgggcgcag gcatcttgac tgggccgcca ccaccggaga caccctgctc
    41581 ggcgcggcgt gggtgatgtt tgtctggtcc ctgctgggtc agctgttgcg cctggtcctg
    41641 ctgaccgcgg gagtcgcgga tccggtgcgg tcccgactgg tcgccggagc tgtcctctgt
    41701 gcggtcgtcg ttctcctcgc gtggggctac gccgaagcga tgcgggtgcc gcgcgtcaag
    41761 aaagtcgagg tcgctatcgc gcggctcggg cccggcttgg atggcctgcg tgtcgcagtc
    41821 atcaccgaca cgcattacgg gccgatcaat cgcgcgcggt ggtcgacagc cgtggtggcg
    41881 cgcgtcaacg agctcagcgc cgacctcgtc tgccatgtcg gcgatatcgc cgacgggacg
    41941 gtcgaggtac gcgagctgca ggccagtccg ctcgcttcgg tgaatgccac gttggctcgc
    42001 gtctacgtca ccggcaatca cgagtacttc agcgaggccg aaggctggct ggactatatg
    42061 gagagcatcg gctgggcggc tctgcacaac cgacacctga tcgtcgagcg cggcggtgac
    42121 cggctcgttg tggccggcgt ggacgacgcc accgcacagt cgtccgatgc ccgcggacac
    42181 cgagcggatc tcgaggccgc ccgcgcgggg gccgacgcca cgctgccggt ccttctcctc
    42241 gcgcatcaac ccaagcaggt ctcccacgcg atgcgcgcgg gcgtcgacct tcagatctcg
    42301 ggtcacactc acggtggtca gatctggccg ttcaatcttc ttgtccgact ggaacaaccg
    42361 gtcgtacatg gtttgagtcg gcacggtgat cggacccagc tctacaccag ccgtggcacc
    42421 ggattctggg ggccgccgtt tcgggtgttc gcgccgagcg agatcacgct gctgactctc
    42481 cgatcgcccc tcaggtagcg cacctccgca actgaggtag tccactaccc cagtgggccc
    42541 ttttccgccg acctagattc ccgaatagat acgcacaact gccttattgg gatcggtagt
    42601 cacgcgtccg ctatgtactc gcgacaacga cgggcgcaac tgcgtccagg tctgtgcaca
    42661 caggagggct ccatgtctat gaacatcaac tacgaaccag ccgcgtcgcg acagttcgaa
    42721 gcgaatccgc cgttcttcgg ctccaaggtc gcctccataa cgacacccgg ctggcagaag
    42781 aacccggtcg ggtcacacct catcatgact gtcggtgtct tcgcgttctg tggcgtgctc
    42841 ggcggcctgt acatgttcat gcagtcgtct gtcgaacgca gttcgacgtc gatgcagcaa
    42901 ttcgccgggt acggcttcct ggccgttggt attgctctcc tggttttggg tacggccatc
    42961 tggtatcggc ggtctcaacg gaagatcctg ctttccgtca cgaccgatga attaactgtc
    43021 aatattcggc caggtgacgt ctactcattc attggcgcga agctgggcac ctggggagtg
    43081 accggcggcg cgacaatggg tttggcgctg cacctccagt ctggcgagcg ccgattcatt
    43141 ctcggcgggc gcgaccgtcg cgttgccgcg ggcacacggc tggaagcaga ggacgtgggc
    43201 tacggcctac cggttgacgt cgacgcctgg ttgtcggccg aagacttcga ggagatcctc
    43261 accatggtca gccgccgcag tgggctggat atccgtcggc ccggaccggg ggacccgatt
    43321 cgctgcttgc tgttcgtcaa ctcgctgaag cttcaagaga tcgggtcgtt ctcattcagg
    43381 aaacagtggg aattctcgcg gtcgatgcac caagcgcggc tggccattga cgttgtccca
    43441 gaagggattc gggtgatcga cccgcagagc ggtgcgctca ttgccgcggt ttcgactgcg
    43501 caggtgactg cgacacccgt gatctatagc cccgagcaag ggaatcactg gtttccggac
    43561 ctcggaaacg tcatcagcga tgtggcgacc aactactggt ccacgtcgcc aggcatgcgt
    43621 gtatccgttc ctggtatggc cccgctgacg atcggatgcc gcgactccgt ttcaggtttg
    43681 gactttcgat tctcgtggcc cggcggcgta cctgtcgtag ctgcgcgcgc tgactacgag
    43741 atctcgggga cggatctgct cactctcacc gagaaattcg ggctgacccg ccatctgcaa
    43801 cgaggcgcat aggctcagct tccaaaaccc gccttgcgca acgcatccgc cattgagccc
    43861 gcaggttgat tcgacggccg ccgcccagca tccttgtccc gacgtggctt accctggcgc
    43921 tgcttgtcag gttcgcgacg gccgccccga tcctgctgcg gcgtgtcatt caggcgcaga
    43981 ctcaaaccga tgcgctggcg ttccacatcg acagcgacga ccttgactcg caccacctga
    44041 cccgaccgca caacctcatg cgggtcggac acaaagtgat cggccatcgc ggagacgtgc
    44101 accaggccat cctgatgcac tccgacgtcg acgaacgcgc cgaacgcagc gacgttggta
    44161 acgacaccct ccaagaccat gccaaccttc aggtcggcca ccttctccac accggcagcg
    44221 aacgtggccg tcgagaacgc gggccgcgga tcgcgtccgg gcttctccag ttcggcgaga
    44281 atgtcggtca cagtcggcat accgaactgg tcatcggcga agtcggcggg ccgaagggca
    44341 cgcaacgacc gctcgttgcc gatgagttcg gcgagagtca cacctgcgcg gtccaggatg
    44401 cgccgcacca ccggatacgc ctcgggatga acaccggacg catcgatggg atcgtcgcca
    44461 ccacggatcc gcaggaagcc cgcgcattgt tcgaatgcct tggggcccaa gcgaggaacc
    44521 ttcagcagcg ccttgcggtt gcgaaacggc cctgtcttgt cgcggtgggc gacaatcgcc
    44581 tcggccaagg attctgagac accggacact cgcgccagca gcggcacgga cgccgtattc
    44641 aggtcgacgc ccaccgcgtt cacggcatcc tcgacgacgg cttccaggct gcgggcgagc
    44701 gtgcctggcg tgacgtcgtg ctggtactgc ccaacgccga tcgacttcgg ctcgatcttc
    44761 accagttccg ccagcggatc ctgcagccgc cgcgcaatcg acacggcccc acgcagagtc
    44821 acgtcgaggc tcggcagctc ttgcgccgca tacgcagacg ccgagtacac cgacgcaccg
    44881 gcctcgctca ccatggcctt cgcaggagct ttggcgcccg cggcgcggat gtcggcaatg
    44941 agttcggcgg ccaaggcgtc ggtctcgcgt gacgccgtgc cattgccgac agcgatcaac
    45001 tcgacgccgt gacgggcgac gagcgccccc agcgctgcct tggccgaatc ccattgcttc
    45061 tgtggctgat gcgggaagat cgcgcaggtg tccaccacct tgccggtgcc gtccacgact
    45121 gccaccttca caccggttcg atagccggga tcaaggccga gcgtggtgcg gttgcctgcg
    45181 ggcgctgcga gcaggaggtc cttgagattc ttggcgaaca ccgcgaccgc gtcttcctca
    45241 gcgcgttggc gcagccggac gcgggcgtcg accctggcag agatgttcag cttggtgcgc
    45301 cacgcccacg cgacggtgcc cgcaagccac ggcgtcgccg atgcattcga ggtcatgtcg
    45361 ataccgaggg tctgggcgat catcgcgtgg tagaactcgt cgtcgccacc gtcgaacgtc
    45421 agcgcaagcg cctcttcctt ctcgccacgc atcacggcaa gcacgcggtg tgacggcatg
    45481 gtctccaacg attccccgaa ctcgaagtag tcgcgaaact tctgcgctgc cgcacctttc
    45541 gcgacatcgt ctgaccaggg cgctgtgcgc agcgaaccgg ccttccagaa cttctcgcgg
    45601 atggcaccga tgagttcggc gtcctcggcc gcacgctcgg cgaggatgtg tcgcgcgccc
    45661 tccagtgctg cggaagcgtc ggctacgtcg tcgcgcagga actctgcggc agcttcctcg
    45721 ggaaccaacg tcggatcggc tagcagccga tcggcaaggg gctcgagccc agcctcgcgt
    45781 gcgatctgcg ctttggtccg cctcttcggc ttgtagggca ggtagatgtc ttcgacacgc
    45841 gatttcgtat cagccgccat caatgcggcc ttcaactcgt cggtaagctt cccccgctcg
    45901 tcgatagagg ccaggaccgc ggcccgacgg tcgtctagct ctcgcagata ctgcaggcgc
    45961 tcctcgaggc tccgcagccg cccgtcatcg agactcccgg tcgcctcttt gcggtaccgg
    46021 gcgatgaatg gaacagtggc gccctcgtcg agtagccgta ccgcagcggc gacctgaacc
    46081 tcctggacag cgagttcctc agcgagacgg gcgtttacag atttcatcgt tgagctccga
    46141 ggcacgcgaa gactctaccg acgaaatacc gacactgcac cgcgccacga acacgagaaa
    46201 gttcgacggg agatgtcgga gacgccgtga cggcgccggt gttgcacgtt caggtgattg
    46261 agtcaggacc catggcggtc ttcgtggtgg gccgatgcac ggcaaccttg cgatgtcgac
    46321 tattccggga tgagttggtc gctatcggtg aacaccaggc tgccggtgtc gagccttacc
    46381 gcccaccgcc gggcaggatc ggcgatgcgt tcggtgccga tatcaacggg gtgtccggct
    46441 gtctgtccga aatcgtcgac aacgaccccg cggcattccg cgtcggtgcc cggatacacc
    46501 cggacccgca tgtccaccgc cacggatgta tcagtgttct tgtcggtcat ctcacgagcg
    46561 cgcttcccca tcttgacccc cagttacgtg aattaacccc gatgtcagta gaccacgctt
    46621 agcccgacgt ggaaaaggga aagcgtgttc atcacgaact cagcgtgacc gccttagagt
    46681 ccagatgtcg agaatggcgt cacggctccg tcccacgggt gagcgaccaa ctagggtcgc
    46741 cacactgaag ggagaaactc atggccaagt acctgttcct caagcactac cgaggcgcgc
    46801 ccgcaagcgt caacgacgtg ccgatggacc agtggacgcc cgacgaggtc caggcccaca
    46861 tccaatacat gaacgacttc gcgaagcgtc tgcaagaaag cggcgaattc gtcgacagcc
    46921 aggcgttggc cccggacggc gcctgggtcc gctccgacgg cgagggcaag ccgccggtga
    46981 ccgacggccc cttcgcggag accaaggacc tgatcgcggg ttggatgatc atcgacgtcg
    47041 actcctacga gcgcgccgtc gagctggccg gtgaattgtc cgcagcaccc ggcgcgggcg
    47101 gcaagcccat ccatgagtgg ctcgaggtgc ggtcgttcct gaccgctgcg ccgaaattca
    47161 ccgattaggt gtctgctgcc gccggggatg cggtggacga ggcccagctg cgggacctga
    47221 tccccggcgt gctggcggcc ctcgtccacc gcggggcgga cttcgcgacc gccgaggacg
    47281 ccgtgcagga ggcgctggtc cgggcggtcg aaacgtggcc gaaccaacgc cccgacgacg
    47341 ccaagggctg gttgatcacc accgcatggc gccgcttcct ggatctcacc cggtccgata
    47401 tcgcgcggcg ccgacgcgag gcccaggtgt ccgacgagcc gccaaccgga ccgaccgcct
    47461 ccgtggatga cacgctccag ctctacttcc tgtgcgcgca cccgagcctc acccccgcgt
    47521 cggcggtcgc cctgacgctg cg
//
LOCUS       DASRJA010000614        20207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_74080,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000614 DASRJA010000000
VERSION     DASRJA010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20207
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74080"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="VFC01_27580"
     CDS             <1..1398
                     /locus_tag="VFC01_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZN83413.1"
                     /translation="RGTFGSGGEFNPMADEIADGADTAAWLRDQPWFTGSFGTVGISY
                     LGFTQWAMLTDPPPEMKAAVITVGPHDLSAPRWGTGSFGLNDFLGWSDMVAHQEDPRR
                     IRALVRQVRARRALTHATSALPIGEAGRALLGTGAPWFESWLEHPEHDDRHWAPLQLH
                     QALERTQIPVLLLSGWQDLFLEQTLAQYQRLREREIPVAVTIGPWTHTQLMTKGAPTV
                     IRESLGWLGAHLADTRRVARKPVRLYVNGHGWRDLDDWPPAMPERVLYLQPAGRLGDT
                     APGADTQASSFTYYPADPTPTIGGRLLSPEGGYRNDTRLAERADVLTFTGDPLPADLY
                     VVGNPLLQLSHSCDNRYNDLFVRISQVDQRGRSRNVSDGYLCATPDSRNMRIELDAIA
                     HRFPAGSRIRVLIAGGSHPRFARNLGTGEPLISGRRLAAATHTVHLGEGGTSRLVLPA
                     GPRPPSDEAFPSHPR"
     gene            complement(1382..1636)
                     /locus_tag="VFC01_27585"
     CDS             complement(1382..1636)
                     /locus_tag="VFC01_27585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83414.1"
                     /translation="MNTTPIRKKFVAGALGAAIAGVTAPALLFLGTGTAQAIPNIGEH
                     HARTITTQRPGHVAIQVAPPLVSPPLVWGQYSSPAFILDD"
     gene            complement(1749..2297)
                     /locus_tag="VFC01_27590"
     CDS             complement(1749..2297)
                     /locus_tag="VFC01_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003913398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HZN83415.1"
                     /translation="MTQPALKEWSAAIHALLDGRQTVLLRKGGIHEKRFAVTASRFLL
                     FPTVAHSHTERVRPEHRDLLDAAAADSTDEAVVLRAGAKVVAAVEVGQPDALDAIAPL
                     HIWTADSVQQDRVDFRPKHRLTVLVVQASPLVEPVRLARAPEYAGCKSWVPLPVDPTW
                     AAPVHDDATLAEISEKVRRSVG"
     gene            complement(2306..2572)
                     /locus_tag="VFC01_27595"
     CDS             complement(2306..2572)
                     /locus_tag="VFC01_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 family protein"
                     /protein_id="HZN83416.1"
                     /translation="MCRNITELRGLQPAATAEEIEAAARQYIRKVSGVTRPTGANVEP
                     FETAVAEVTEITTRLLAGLAPRRQPPKTVPPLRRPEVRARLAAR"
     gene            2595..3365
                     /locus_tag="VFC01_27600"
     CDS             2595..3365
                     /locus_tag="VFC01_27600"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZN83417.1"
                     /translation="MADDILLIETEDRVRTLTLNRPQSRNALSAELRRQFFTALRDAE
                     GDGDVDVVIVTGADPVFCAGLDLKELGDSSQLPDISPQWQPMTKPVIGAINGAAVTGG
                     LEIALYCDILIASEQARFADTHARVGLLPTWGLSVRLPQKVGVGLARRMSLTGDYLSA
                     PEALRAGLVTEVVPHDELLPAARKVAASIVGNNQNAVRALLASYHRIDAAQTDAGLWI
                     EAMSAKQWMNSATGNDIAANRDAVLQRGRAQVRSREPL"
     gene            3467..3940
                     /locus_tag="VFC01_27605"
     CDS             3467..3940
                     /locus_tag="VFC01_27605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83418.1"
                     /translation="MSSEWYENYYKMRAAIFEESTEAARSARAFYRDIVGADAIVRLT
                     VALRLQVTEGGQDWAIEVAEWERTAEFLDCYETQPLDDEERYWLLELIIASFDDHLRI
                     QGPDERVAARVRRHLIESFEAHRATVEYWACLAEPATTRFAITPLMRELWEFCRT"
     gene            4246..5484
                     /locus_tag="VFC01_27610"
     CDS             4246..5484
                     /locus_tag="VFC01_27610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83419.1"
                     /translation="MNITHTRDARRRWRGASPGVRRWLIVLMLALGVLLTLNAPAAHA
                     APTLTASENPVFIPYSKDTEDITLAWTVEPWQLLATLTVTESGTPAPVIDKTVGPTPP
                     PGGEPLTVTYGKTYTAELKDVITQQPLVAPLTITTDRIDIKIDMGCLVQCITEPKVEP
                     HGGWAKFTINTTETASMTLEASKTKPNANGTWNNPKDVAASNATILPTQNWTAPLPNL
                     DSNTTYYYVVRAHDTNGNEQIKTGSFKTLTRRVEVTFAEIQMIDDSDDLGDCDCFFWF
                     NAGDETPKPWGDFQHQKSIASGTTVHPNVTFTIDNAPSEILIRALGYDDDSAFGSLCS
                     AGTGYLPYTGNATWITTSGMAEDCQQLTGDQVTVSVSHQGQPAAPDAVDEQFTEQFTI
                     SAGLGPLVYKVHGSYKVTYA"
     gene            5527..6018
                     /locus_tag="VFC01_27615"
     CDS             5527..6018
                     /locus_tag="VFC01_27615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83420.1"
                     /translation="MNSIDSRKKFVPPWTLPAAGVALVILVTGCAGGGVTGSVTTSTP
                     PVPSVVALMPNDHGYVRVESKSGNMRCSIAANLVACQTSADNWPRHPDGTPFHCASVT
                     AGGEFHWVDADLGALEGRITMDYQTYRAQGWTISAAAGETTFTNDRSGHGMVVSTADV
                     QPF"
     gene            6084..6653
                     /locus_tag="VFC01_27620"
     CDS             6084..6653
                     /locus_tag="VFC01_27620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN83421.1"
                     /translation="MNKAHEACGSDEWRQAIREVILPWALGETDLGDNVLEVGPGYGA
                     TTDVLSQSVPRLTSIEIDDELAAMLSDRFADVPSVQIVHGDATSLEYTDDTFSGAACF
                     TMLHHVATNELQDLLFAEIARVLKPGAPLVASDSLASDELEAHHEGDTYNPVDPSSLP
                     SRLATAGFEGVEVRTNPFGWAAIARVAKK"
     gene            6742..7092
                     /locus_tag="VFC01_27625"
     CDS             6742..7092
                     /locus_tag="VFC01_27625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83422.1"
                     /translation="MTTTRTRFAVLMGAAIVGAAAAALFLGADTARAVPEPADRGGAV
                     TLDYLPAPRECGSCGASNPQPDTSGYPDTRLNPPPGLNPPPGLGVGLGGPDTLPPAGA
                     FNPQTDPPSTPAGP"
     gene            7205..8653
                     /locus_tag="VFC01_27630"
     CDS             7205..8653
                     /locus_tag="VFC01_27630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN83423.1"
                     /translation="MTAVPSWDIVGPAETDAELARAAAAGDREAFAGIYDRYADRLHD
                     FCIGMLRDRDGAADCVQDVFCTAATRLPQLRDPDKLRPWLYAIARNEALRRIRDRRRE
                     TPSDELPEAVSHEPGPETLAARTELADLIAEAAGGLSDRDRSVLELAYRHGLDGPDLA
                     DALGVSPNHATKLVYRLRETVERSLGALLISRRAQNTRGGCRELAAILDGWDGQFSVL
                     MRKRISRHIESCLTCDEERRRVVSPVALLGAAPAFIPAPAPLRERTLSEIQLTASATA
                     IEANTTDPKPEASAPGGTPPIAVENVDDVDDARSDRTRRVALVIGLFAATLMASLGLT
                     IAWLQEKTTTIEPADMSTATQPISATPSGQPSQTPSNPIPAPPVVSAESTTPAVSTPP
                     VTETSTPTNVFTAPPPTLETDSPVSEPSVQPTPRVSEPPAPPTRRTSSGPTNSSPPRT
                     TRSQQPPPGIVAIPTGNPDPPKRRVTRRGTGL"
     gene            8813..9115
                     /locus_tag="VFC01_27635"
     CDS             8813..9115
                     /locus_tag="VFC01_27635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83424.1"
                     /translation="MASVSFSHIGYITAIEPGDTHHVSWNNAAAQRVWAFSVDAMLSL
                     KIPPAIGASARVEITRVEYREVNNGAGNLEKEIHWWIKNTGTTDASYAIHMATIKE"
     gene            9127..9345
                     /locus_tag="VFC01_27640"
     CDS             9127..9345
                     /locus_tag="VFC01_27640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83425.1"
                     /translation="MKLLVLCDSAGNIQSVAVPNPELPGKLQLEVEGGPTARELEVDE
                     REIGREDLLGQKGAKAQGRALQKLRELM"
     gene            9589..10122
                     /locus_tag="VFC01_27645"
     CDS             9589..10122
                     /locus_tag="VFC01_27645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83426.1"
                     /translation="MTTTHANYGSNNDDNAAIVIGNDSQAGAGLFGDNNDDNTAVAIG
                     TDSGASAGANVVAVSFYNNNTGNKALAINGGTALAGFNGSDNDNNSATAFGPDSLAAA
                     GGGEVFGLTLGNFNTGNEATATQGGEALAGVNGDFSDNNTATASDAGSLASAGSVGFN
                     LDDYDAAATGGQTDVQP"
     gene            10242..11009
                     /locus_tag="VFC01_27650"
     CDS             10242..11009
                     /locus_tag="VFC01_27650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83427.1"
                     /translation="MTTTYDTDPNTQTVAAAWASSPLGQPERELRHGFATAEPAEFDE
                     PKSPAKRAILAAALAAGVIGGAVLGVSLFDFSDSSQPTVVEQGAVPGHAVVVGPNGTA
                     PAPSTQVVPEQKAAPSVVVPAPQRGPGPVVVATPPVAPPPAAPPSEPPVVVDVPNPDY
                     PPLPPKPEDPKPEPPNPVPPVPDDLDFKLPDPPKPDPKPVPDDLDFKLPDPPKPDPKP
                     VPPVFLPDLPLAPSPQLNPQPEPPSLPDLVLQAPVKS"
     gene            11042..12412
                     /locus_tag="VFC01_27655"
     CDS             11042..12412
                     /locus_tag="VFC01_27655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN83428.1"
                     /translation="MTAASYWDIVGPSGSDAELARASAAGDRTAFADIYDRYADRLHD
                     FCVGMLADRDGAADCVQDVFCIAATRLPQLRDPDKLRPWLYSIARNEALRRIRERRRE
                     APSEELPDTASGEPGPETLAARTELADLIADAAGGLSDRDRSVLELAFRHGLDGPDLA
                     EALGVSASNANTIVHRLRGTIERSLGALLVSRRVRNSGGCAELSAILDGWDGHFNVLM
                     RKRISRHIESCQTCDEERRRLVSPVALLGAAPAFIPAPAWLRERTLSEVQLTSAASPI
                     GDGGADAGRGTRNSLLPVTVFVVALLAALGLTFVWINLQETTTIQSPIEVSETAPPPA
                     APSPVAPPPPPPPPTASVKPPPPATAPPPRRTPTIEQPSAEPTPVAPPPISEPTPVEP
                     PPPPPPPPPPPPWPPWPEWPQWQWPDPQGPRPGGGDLTGPGDLVLPGQGGSNGSRPCS
                     AIQCPP"
     gene            12492..13859
                     /locus_tag="VFC01_27660"
     CDS             12492..13859
                     /locus_tag="VFC01_27660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZN83429.1"
                     /translation="MTAVPSWDLVDPRHTDAELVGASMAGDRAAFAQIYDRYADRLHD
                     FCVGMLRDFDAAADCVQETFCLAATRLPQLREPDKLRPWLYAIARNESLRRLRDRQRE
                     TPSDELPDIESDDASPDTLAARTELADLIAEAAGGLTDRDRAVFDLAFRHGLNGPDLA
                     DALGVSHTNANTMVQRLRGTIERSLGALLVCRRRNPGGCAELGQILAGWDGQFTVLMR
                     KRVSRHIESCPNCDEERRRLVSPAALLAATPAFIPAPKWLRDKTLGDIQLVCHASAMD
                     GLSDDEAASHHDDSGASDVDGASGEQGRRRVLLAALLIAVLGTTAGLMLAWLNREPTS
                     ILPTEISGTSPTSQATFPISSSPTHTTAPVPTTEPVAPLPPAADLPQVPGTAATPELI
                     PSEPPPYSPPSEPAPRTRPTAITRSAPSYSPTRTADPTSPGGNLGGGNQGGSDRNPPP
                     GGSAP"
     gene            13957..14490
                     /locus_tag="VFC01_27665"
     CDS             13957..14490
                     /locus_tag="VFC01_27665"
                     /inference="COORDINATES: protein motif:HMM:NF025590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HZN83430.1"
                     /translation="MHINNSRKKFVTAAFAAIAGAVALTGCQSPFTSAEPDYEALESY
                     VFDEVNGDFPSQISREVSGVDCPRQADTVTSGESFVCTVDVDGHAVRVEVTAKNEGLD
                     FNTLDMVYDLGPAAQDFADQISNEVEFPITLSCGEGLKVVPIGDSFECTAADEYGDTR
                     TIRITANPDGEFWEVLD"
     gene            14702..15541
                     /locus_tag="VFC01_27670"
     CDS             14702..15541
                     /locus_tag="VFC01_27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZN83431.1"
                     /translation="MTPSIVLAVLFAAVLHAIWNSLAHAVSDRLVGFALIGLVDAVGG
                     AALAAAGGLPPAGAWPFIVASALTHTAYNLFLLASYQLGEFSQMYPLARGTSPWVVAL
                     VSIVVLHRALSITELAGVLAVSAGLIGLVLIGGRPGCKDLPALTAALLTGLMISSYTV
                     IDGVGVTHAPLLAYAGWMFLLQAPPIVMIALIRRRGKLVAAMRACAFPGLTGGAISLV
                     AYTIVLWAQLSGALATIAALRETSIVFGALIGSVFLGERLGPRRAIAAAVVLGGVVLI
                     SLP"
     gene            complement(15545..16858)
                     /locus_tag="VFC01_27675"
     CDS             complement(15545..16858)
                     /locus_tag="VFC01_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZN83432.1"
                     /translation="MPVAPATTRRIAGLALPALGVLAAEPIYLLFDIAVVGRLGALPL
                     AGLAIGGLILSTLSSNMTFLSYGTTARSARFFGAGNRAAAVGEGVQATWLALGLGALI
                     VAAVEIFAVPLVSALAAGGNIAEAALPWVRIAIVGVPAILVSAAGNGWMRGVQDTARP
                     LRYVVAGFAVSAVLCPLLVYGWLGMPRLELVGSAVANLVGQWVAALLFLRSLFAERVP
                     LGIQPAVLRAQVVMGRDLVLRTMAFQACFVSAGAVAARFGAAAVAAHQVVLQLWNFLA
                     LVLDSLAIAAQSLVGAALGAGELAHAKSVAWRVTIFSAVASAVLAGIFAVGASVFPSV
                     FTNDRSVLDAIGVPWWFMVAQLPIAGIVFALDGVLLGAGDAKFMRNATLISALVGFLP
                     LIWLSLAFGWGLLGIWSGLSTFMVLRLAFVGWRAFSGHWLVPGTG"
     gene            complement(16872..17834)
                     /locus_tag="VFC01_27680"
     CDS             complement(16872..17834)
                     /locus_tag="VFC01_27680"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HZN83433.1"
                     /translation="MGERVDAQQVADLLDAAATVSVVCHVYPDADTIGAGLALALVLD
                     HAGKAVEVSFAAPAELPESLQSLPGGHLLVGPDEMRRDADLVVTVDIPSVNRLGALSE
                     LADPGREVLVIDHHASNQLFGTANYVDPSADSTTMLVAELLDAWGKPIEPGVAHCLYA
                     GLTTDTGSFRWASARAHRLAARLVELGVDNASISRTLLDSHPFAWLPMLSRVLSSAQL
                     LPDAAGGRGLVYAIVGHQEWANARPEEVESIVDIVRTTQQAEVAAVFKEIEPQHWSVS
                     MRAKSYDLAAVASAFGGGGHKLAAGYSATGSADDVVAALYAALG"
     gene            complement(17845..18342)
                     /gene="rbfA"
                     /locus_tag="VFC01_27685"
     CDS             complement(17845..18342)
                     /gene="rbfA"
                     /locus_tag="VFC01_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZN83434.1"
                     /translation="MADPARARRLAKRISTIVASAIEYEIKDPRLAGVTITDAKMTGD
                     LHDATLFYTVFGRNLEDEPDYAGVAAALESATGVLRTKVGAALGVRFTPTLAFERDTV
                     PDTAHRMEELLARARAADEDLARVREGATHAGDADPYRVTGVEESGGDRDDSEDMGDG
                     NRPDD"
     gene            complement(18335..>20207)
                     /gene="infB"
                     /locus_tag="VFC01_27690"
     CDS             complement(18335..>20207)
                     /gene="infB"
                     /locus_tag="VFC01_27690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZN83435.1"
                     /translation="RKSKRAKRAEYENMQAPVVGGVRLPHGNGETIRLARGASLSDFA
                     EKIDANPASLVQALFNLGEMVTATQSVGDETLELLGSEMNYNVQVVSPEDEDRELLES
                     FDLTYGEDEGGEEDLETRPPVVTVMGHVDHGKTRLLDTIRQANVREEEAGGITQHIGA
                     YQVEVDLDGTIRPITFIDTPGHEAFTAMRARGAKATDIAILVVAADDGVMPQTVEAVN
                     HAQAAEVPIVVAVNKIDKEGADPQKIRGQLTEYGLVPEEFGGDTMFVDISAKQGTNIE
                     SLLEAVVLTADAALDLRANPDMEAQGVAIEAHLDRGRGPVATVLITRGTLHVGDSVVA
                     GDAYGRVRRMVDEHGEDVEAALPSRPVQVIGFTSVPGAGDNFLVVDEDRIARQIADRR
                     SARKRNALAARTRKRISLEDLDSALKETSQLNLILKGDNAGTVEALEEALLGIQVDDE
                     VELRVIDRGVGGVTETNVNLASASDAIIIGFNVRAEGKATELANREGVEIRYYSVIYQ
                     AIDEIESALKGMLKPVYEERELGRAEIRAIFRSSKVGNIAGCLVQSGIIRRNAKARLL
                     RDNVVVTENATVQSLRREKDDVTEVRDGYECGLTLSYSDIKEGDVIETYELVEKERG"
BASE COUNT         3591 a         6956 c         6427 g         3233 t
ORIGIN      
        1 cgtggaacat tcggctctgg cggcgaattc aacccgatgg ccgacgagat cgccgacggc
       61 gcggacaccg ccgcctggct gcgcgaccaa ccgtggttca cgggttcctt tggcactgtt
      121 gggatctcct acctgggctt cacccagtgg gccatgctga ccgatccgcc gccggagatg
      181 aaggccgcgg tcatcaccgt cggcccgcac gacctgagcg ccccgcgctg gggtacaggc
      241 tcattcggcc tcaacgactt cctcggctgg tccgacatgg tcgcccacca ggaggatccg
      301 cgccgcatcc gcgcgctggt ccgccaggtc cgggcccgac gggcactcac ccacgccacc
      361 agcgcgctgc cgatcggcga ggcgggcagg gcgctgctag gcaccggtgc gccgtggttc
      421 gaatcgtggc tcgagcatcc cgagcacgac gaccgacact gggcgccgct gcagctacat
      481 caggcgctgg agcgcacaca gatcccggtc ctgctgctca gcggatggca ggacctgttt
      541 ctcgaacaga cgctggcgca gtatcaacgg ctgcgtgagc gtgaaatacc ggtcgcggtc
      601 acgatcgggc cgtggaccca cacgcagctg atgaccaagg gtgccccaac ggtcatccga
      661 gaatcgttgg gctggttggg cgcccatttg gcggacaccc gccgggtggc gcgcaaaccg
      721 gtgcggcttt atgtcaacgg gcacggatgg cgggacctgg acgattggcc gcccgcgatg
      781 cccgaacgcg tcctgtatct acagccggcg ggccgactcg gcgacaccgc gcccggtgcg
      841 gacacgcagg cgtcgagctt cacctactac cctgccgacc cgacgccgac catcggcggt
      901 cggctgttgt cgccagaagg cggctaccgc aacgacactc ggctcgccga gcgcgcggat
      961 gtgctgacct tcaccggtga cccgctgccc gcagatctgt acgtcgtcgg gaacccgctg
     1021 ctgcagctgt cccactcgtg cgacaaccgc tacaacgacc tgttcgtccg gatcagtcag
     1081 gtcgatcagc ggggccgctc ccgcaacgtg agcgacggat atctttgtgc cacaccggat
     1141 tcacgcaaca tgcggatcga actcgacgcg atcgcgcacc gcttccccgc cgggtctcgg
     1201 attcgggtgt tgatcgccgg cggttcacac ccccggttcg cgcgcaatct cggcaccggc
     1261 gaaccgttga tcagcgggcg gcgactggcc gcggcgacgc ataccgtgca cctgggcgag
     1321 ggcggcacct cgcggctcgt gctgcccgcg ggcccgcgac cgccgtcgga cgaagctttc
     1381 cctagtcatc caagatgaaa gcgggagaag agtattgacc ccacacaagt ggcggcgaaa
     1441 ctagcggcgg cgcgacctgt atcgcgacgt gtccgggccg ttgagttgtg attgttctgg
     1501 cgtggtgttc gccgatgttc ggtatcgctt gcgcggtccc tgtgccaaga aacagcagtg
     1561 ctggtgccgt cacgcctgcg atagcggcgc cgagggcacc cgcgacgaac ttcttgcgga
     1621 tgggggtggt gttcatgatg tgctccttgt cgtgtcgccg ccctggatgg gccgcgtgta
     1681 cacgtaggag gagccatctg ccccgcgtct gacacttttt gggaaactcg ttcggccggc
     1741 ctcggcggtc aacccactga gcgacgtact ttttcggaga tttcggccag cgtggcgtcg
     1801 tcgtgcaccg gcgccgccca ggtcgggtcc accggcaacg gaacccaact cttgcagccc
     1861 gcgtactcgg gcgcacgggc cagccgaacg ggctcgacta gcgggctggc ctgcaccacg
     1921 agcacggtga gccgatgctt tgggcggaaa tccacccgat cctgctggac cgaatcagcg
     1981 gtccagatgt gaagcggcgc gatcgcgtcg agcgcatcgg gctggccgac ctcaacggcg
     2041 gcaacgactt tggcgccggc acgaagtacg acagcctcgt ctgtgctgtc cgccgccgcg
     2101 gcgtcgagca ggtcacgatg ctcgggtcgc acccgttcgg tgtggctatg cgcgacggtc
     2161 ggaaacagca ggaaacggga agccgttacc gcgaaccgct tttcgtgaat gccgcccttg
     2221 cgcagcaaca cggtctgcct gccgtcgagc agcgcatgga tcgccgcgct ccactccttg
     2281 agcgccggtt gggtcaagat tgtggtcacc tggctgcgag tcgcgcccgc acctccggcc
     2341 gccgcagcgg gggaacggtc ttcggcggct gccgtcgcgg cgccagacca gccagcagcc
     2401 gcgtggtgat ctcggtgact tcggcgaccg cggtctcgaa gggctcgacg ttggcgcccg
     2461 tcgggcgtgt cacaccgctg accttgcgga tgtactggcg cgcggccgcc tcgatctcct
     2521 cggctgtcgc cgccggctgc aggccgcgca gctccgtgat atttcggcac ataccgccac
     2581 gataggttga tctgatggcc gacgacatct tgctcatcga gactgaagac cgggtgcgga
     2641 ccctgacgct gaaccggccg cagtcgcgca acgcgttgtc ggccgagctc cgcaggcaat
     2701 tcttcaccgc gctgcgcgat gcggagggcg atggcgacgt cgacgtcgtc atcgtgaccg
     2761 gcgccgaccc ggtgttctgc gcgggcctgg atctcaagga gctgggcgat tcatcgcagc
     2821 tgcccgacat ctcgccgcag tggcagccga tgaccaagcc ggtgatcggc gcgatcaacg
     2881 gcgctgccgt gaccggcggc ctggagatcg cgctgtactg cgacatcctg atcgcctccg
     2941 aacaggcacg gttcgccgac acccacgccc gcgtcgggct cctccccacc tgggggttga
     3001 gcgtgcgcct gccacagaag gtcggtgtcg ggctggcccg ccggatgagc ctgaccggtg
     3061 actatctgtc cgcgcccgaa gcgctgcgcg ccgggctggt caccgaggtg gtgccacacg
     3121 acgaattgct gcccgcggcg cgcaaggtcg ccgcgtcgat cgtcggcaac aaccagaacg
     3181 cggtgcgtgc gctgctggcg tcgtatcacc gcatcgacgc cgcgcagacc gacgcggggc
     3241 tgtggatcga agcgatgtcg gcgaagcaat ggatgaattc agcgacgggc aacgacatcg
     3301 ccgccaaccg cgatgcggtg cttcagcggg gccgcgcaca agtgcgctcg cgcgaacctc
     3361 tttagaaaat ctctcccgaa aagtgtgcga gcggcgcggg gtggcactcc tacccgtgag
     3421 agcggcccat cgttgaggcg ctgcccaaaa gttggtgagg gaaatagtga gttccgagtg
     3481 gtacgagaac tactacaaga tgcgcgcggc catctttgaa gagtcaacag aggccgcaag
     3541 gagtgcgcgt gcgttctacc gggacatcgt cggcgctgac gccatcgtgc ggctcacggt
     3601 ggcgctgagg ctgcaggtga ctgaaggcgg gcaagattgg gcaatcgaag ttgcggagtg
     3661 ggagcgaacc gccgagttcc tggactgcta cgagacgcag ccactggatg acgaggaacg
     3721 ctactggcta ctggagttga tcatcgcgtc gtttgatgat cacctgcgca tccaggggcc
     3781 ggacgaacgg gtggcagccc gagttcggcg tcacctgatc gagtcgtttg aagcgcatcg
     3841 cgccacggtg gagtactggg catgcctggc ggaacccgcg acaactcgct tcgctatcac
     3901 accgttgatg cgcgagttgt gggagttttg ccgaacgtaa cgctgcatct gacgggtcgg
     3961 gcgaggtcgc cgtgaaggcg gatggtgttg tgccgtgctc acggagcgcg ggatccacaa
     4021 atcgcgtatc ccaacatatc aacgctcaca gcgcatttcg gcatcaacag ccacgcggtc
     4081 tgggccgcct cggcactgtg tgatcgcggt ctgcctcctt ttgtagtcgc ccgtccgggg
     4141 acgctccgaa gaagccctcg aaaaagtgtc agaccgacag ggagtggcgg ctcatacagg
     4201 tgagcgctgt ccatctgggc cgcggcaatg caaaaggagc acaacatgaa catcacccac
     4261 acccgtgacg ctcgccgaag atggcgcggc gcttcccccg gcgtgcgacg ctggttgatc
     4321 gtcctgatgc tggcgctggg cgtgctgctc acgctcaacg cgccggcagc ccatgccgcg
     4381 ccgacgttga cggcgagcga gaacccagtg ttcatcccct acagcaagga cacagaagac
     4441 atcacgctgg catggaccgt ggagccctgg cagctgctgg cgaccttgac cgtcaccgag
     4501 agcggtacgc ccgcaccggt gatcgacaag acggtgggcc caacaccgcc gcccggcggc
     4561 gagccgctga cggtcaccta cggcaagacc tacaccgctg agctcaagga tgtcatcacc
     4621 cagcaaccgc tcgtcgcgcc gctcaccatc accacggacc ggatcgacat caaaatcgac
     4681 atgggctgcc tggtgcagtg catcaccgag cccaaagtgg agccgcacgg gggctgggca
     4741 aaattcacca tcaacaccac cgagacggcg agcatgaccc tcgaggcgag caaaacgaag
     4801 cccaacgcca acggcacgtg gaacaacccc aaagatgtgg ccgcctccaa tgccacgatt
     4861 ctgccgaccc agaactggac ggcgccgctg cccaacctgg actcgaacac gacctactac
     4921 tacgtcgtgc gcgcccatga caccaacggc aacgagcaga tcaaaacggg cagcttcaaa
     4981 acgctgaccc ggcgcgtgga ggtcaccttc gccgagatcc agatgatcga cgacagcgac
     5041 gacttaggcg actgcgactg cttcttctgg ttcaacgccg gcgacgagac accgaagccc
     5101 tggggtgact tccagcatca gaagagcatc gcaagcggca cgaccgtgca cccgaacgtt
     5161 acgttcacca tcgacaacgc cccgagcgag atcctgatcc gtgcgctcgg atacgacgac
     5221 gacagcgcct tcggttcgct ctgctccgcc ggcaccggct acctgccgta caccgggaac
     5281 gcgacgtgga taacgacctc gggcatggcc gaggactgcc agcagctgac cggggaccag
     5341 gtcacggtgt cggtctccca ccagggacaa cccgctgcgc cggacgccgt tgacgagcag
     5401 ttcacggagc agttcacgat ctccgcggga ttggggccgc tcgtatataa ggtccacggg
     5461 tcctacaagg tgacatatgc gtaacccggg cgtctcgaga aagtgtcaga cgaccgggaa
     5521 cacaccttga acagcatcga cagccgcaag aagttcgtcc cgccttggac actgccggcc
     5581 gccggtgtgg ccctcgtcat cctcgttact gggtgcgcgg gcggcggcgt gaccggctcc
     5641 gtcactacct cgacgccgcc ggtgccgtcg gtcgtcgcgc tcatgcccaa tgaccacgga
     5701 tacgtgcggg tggaatcgaa gtcgggaaac atgcgctgct cgatcgctgc caacctggtg
     5761 gcctgccaaa cctctgccga caactggcct cgacatccgg acggaacgcc ttttcactgc
     5821 gcgagcgtga cagcaggtgg cgaattccat tgggtcgacg ccgatctcgg ggcactcgag
     5881 ggccgaatca cgatggacta ccaaacctac cgagcgcagg gctggacgat cagcgcggct
     5941 gctggtgaaa ccacattcac gaacgaccgc agtggtcacg gcatggtcgt cagcacagca
     6001 gacgtacaac cgttctgacg cgaacgacgc ggtgctgcaa cggggtggcg cacacatgcg
     6061 ctgaactttc gtacgctgcg accgtgaaca aagcgcacga ggcctgcggc agcgacgagt
     6121 ggcggcaggc gatacgagaa gtcatcctgc cctgggcgct gggcgaaacc gatctcggtg
     6181 acaacgtgct cgaagtcggg cccggctacg gtgcaacgac cgatgtgctg agccagtcgg
     6241 taccgcggct aacctcgatc gagatcgacg acgagctcgc ggccatgttg tcggaccgat
     6301 tcgcggatgt gccgtcggta cagatcgtgc acggcgacgc gacgtcactt gagtacaccg
     6361 acgacacctt ctccggcgcg gcgtgcttca cgatgctgca tcacgtcgcg acgaacgagc
     6421 ttcaggacct gctgttcgcc gaaatcgccc gggtgctcaa gccaggcgcc ccgctggtgg
     6481 ccagcgacag cctggccagc gacgagctcg aggcccacca cgagggcgac acctataacc
     6541 ccgtcgaccc gtcgtcgttg ccatcacggc tggccacggc cgggttcgag ggtgtcgagg
     6601 tgcgcacgaa tccgtttggt tgggcagcca tcgcgcgggt cgccaaaaaa taggtggaaa
     6661 aagtgggcga ggagcgcgtg gtcggcgctc ctatccataa ccgcagccta accagggcag
     6721 cgatcacctc aggagaacgc catgaccacc acccgcacca gattcgccgt cctaatgggt
     6781 gctgcgatcg tcggcgcggc ggcagctgcc ttatttctcg gtgcggatac cgcacgggcc
     6841 gtgcctgaac ccgccgatcg tggcggggcg gtgaccctcg attaccttcc cgcgccgcgt
     6901 gaatgcggtt cgtgcggggc aagtaatccg cagcccgata cgtcggggta ccccgatacc
     6961 cgcctcaacc cgccacccgg cctgaatccc ccgccgggtc ttggggttgg gctcggtggt
     7021 cccgataccc tgccgccggc gggagcattc aatccgcaga cagatccccc gagcaccccg
     7081 gctgggccgt agggcgcgtt cgacggaagg ctcacggcgc aacgggcacg actcgggtct
     7141 tgcttatgcc caaacagcct ggtcgaatat cagcagcacg acctttgtca tgggaagcac
     7201 agtcatgacg gcagtaccca gctgggacat cgttggccca gcggagaccg acgcggagct
     7261 cgcccgcgcc gccgctgccg gtgaccgtga agcgttcgca ggcatctatg accgctacgc
     7321 cgaccggctc cacgatttct gtatcggaat gctgcgcgac cgcgacggcg ccgccgattg
     7381 cgtccaggat gtgttctgca ccgccgcgac ccgactgccg cagctgcgag atccagacaa
     7441 gctgcgcccg tggctgtacg cgatcgcccg caacgaagcg ctgcgccgca tccgggaccg
     7501 gcgccgcgaa acaccttctg acgaactgcc tgaggccgtg tctcatgagc ctggccccga
     7561 aaccctggct gcccgcaccg aattggccga tctgatcgcc gaggccgccg gcgggctgtc
     7621 cgaccgcgac cgctcggtgc tcgagttggc ctaccgccac ggcctggatg gacccgacct
     7681 cgccgatgcc ctcggggtga gccccaacca tgccaccaag ttggtgtacc ggttgcgtga
     7741 gaccgtcgaa cgttcccttg gcgcactgct gatctcacgc cgcgcccaga acacccgggg
     7801 cgggtgccgc gaactcgccg cgatcctgga cggctgggat ggacagttca gcgtgctgat
     7861 gcgcaaacgc atttcgcgcc acattgagtc ctgtctcacc tgcgacgagg agcggcgacg
     7921 ggtggtcagc cccgtcgcgc tgctcggcgc cgctccggcg ttcatccccg cacccgcacc
     7981 gctgcgcgaa cgcaccctca gcgaaattca gctcaccgca tcggccaccg cgatagaggc
     8041 caacacgacc gatcccaagc ccgaggcctc ggcgcccggc gggaccccgc cgatcgcggt
     8101 cgagaatgtc gacgatgtcg acgacgcccg cagcgatcgc acccgccgcg tggcgctggt
     8161 gatcgggtta ttcgccgcca ccctgatggc gtcgctgggg ctgaccattg cctggctgca
     8221 agaaaagacc accaccatcg aacccgccga catgagtacg gccacccaac caatcagcgc
     8281 cacaccgagc ggccaaccga gccaaacccc aagcaaccca attccagctc cgccggtcgt
     8341 gtcggcggaa tcgaccactc cagccgtatc gacgcccccc gtcaccgaaa cttctacgcc
     8401 aacaaatgtc ttcaccgcac cgccgccgac gttagagacg gactcacccg tgtccgaacc
     8461 gtcggtgcaa ccgactccgc gcgtgtcgga gccgcccgct ccacctaccc ggcggacttc
     8521 ctccgggccg actaactcgt caccgccgcg gaccacccgt tcgcagcagc ctccgccagg
     8581 aattgtggct attcccacag ggaatccgga ccctccgaaa cggcgcgtaa cgcgaagggg
     8641 cacagggctc taacccaccc ttgcagccag cgagtctcag gcgctacggc agccgttcaa
     8701 gaaaatactc ggaaaactgt ccgggtggcg ctagatcgac gctcctacac atgacagcgg
     8761 ccatccgggc ccctgcatta cccagaatcg attctgtgaa ggaggaagat ccatggccag
     8821 tgtcagcttt tcccacatcg gctacatcac cgccatcgag ccgggcgaca ctcaccacgt
     8881 ctcgtggaat aacgctgcgg cccaacgggt gtgggcattc agcgtggatg cgatgctctc
     8941 gctgaagatc ccgcctgcaa tcggggcgtc tgcacgcgtc gagatcacgc gcgtcgagta
     9001 tcgcgaggtc aacaacggcg cgggcaacct agaaaaggag atccactggt ggatcaagaa
     9061 tacggggacg accgacgcca gctacgccat tcacatggcc acgatcaaag agtgagaaga
     9121 cgtgccatga aactgcttgt gctgtgcgac tccgccggga acatccagtc ggtggcagtc
     9181 ccgaacccgg aactgcccgg caaactgcaa ttagaagtcg agggcggacc gacggcccgc
     9241 gagctcgagg ttgacgaacg cgagatcggg cgggaggacc tgctcggcca gaagggcgcg
     9301 aaagcgcagg gacgagctct ccagaagctc cgtgagctga tgtagtacac gccgtcgccg
     9361 tacgcaacgg gcacacccgt acgtcgtgag gttatcgctg accatcaggg ccaccgtgag
     9421 gggccctgat ggttttgcga tagggcgagc cagtctgact ctgccgcagt ctgattcgcc
     9481 aagttctctc aacgttctgg tcctccaaga attgtccgcc cggcggtggt ggtggctctt
     9541 acccaggaat gcgggcatcg ggtcccaccg tcgagaagga gcacgttcat gaccaccacc
     9601 cacgccaatt acggcagcaa caacgacgac aacgccgcca tcgtcatcgg caacgacagc
     9661 caagccggcg ctggcctctt cggtgacaac aacgacgaca acaccgccgt cgccatcggc
     9721 accgatagcg gcgcttccgc aggcgcgaac gtggtcgctg tcagtttcta caacaacaac
     9781 acgggcaaca aagcccttgc catcaacggc ggtacggcac tcgccggttt caacggcagc
     9841 gacaacgaca acaacagcgc caccgccttt ggacccgaca gcctggccgc ggcaggcggc
     9901 ggcgaggtct tcgggcttac tctgggcaac ttcaacactg gcaacgaagc caccgccacg
     9961 cagggcggcg aggctttggc tggcgtcaac ggtgacttca gcgacaacaa caccgccacc
    10021 gcctccgacg ccggcagcct ggcctcggcc ggctccgtcg gcttcaacct cgacgactac
    10081 gacgctgccg cgaccggtgg gcagaccgac gtccagccat aggagtgggt tggctgagaa
    10141 aaaaataagt tggaaaaagt gtgcgaggag cgcgtggtcg tcgctcctac ctgtagccgc
    10201 agcccaaccg gggcagcaaa cccacaccaa ggaaaccggc catgaccacc acgtacgaca
    10261 cagacccgaa cacccagacg gtcgcggccg cgtgggcgtc gagcccactg ggtcaaccgg
    10321 agcgcgaact ccgacacggc ttcgccaccg ccgagccggc cgaattcgat gagccgaagt
    10381 cgcccgccaa gcgggcgatt ctcgcggcgg cgctcgcggc cggcgtcatc ggaggcgccg
    10441 tgctcggcgt ctcgctgttc gacttcagcg actcctccca gcccacggtt gtggagcagg
    10501 gagctgtccc cggacacgcc gtcgtcgtcg gcccaaacgg aaccgcacct gctccgtcca
    10561 cacaggttgt gcccgagcag aaagcggctc cgagcgtcgt cgtgccggcg cctcagcgcg
    10621 ggccgggtcc ggtcgttgtc gccacacctc cggtagcccc acctccggcg gccccgccga
    10681 gcgagccccc ggtggtcgtc gatgtcccga atccggacta cccgccgctt ccgcccaagc
    10741 cggaggatcc gaagcccgag ccgccgaatc cggtgccgcc ggtgccggac gatctggatt
    10801 tcaagctgcc ggacccgccg aagccggacc ccaagccggt cccggacgat ctggatttca
    10861 agctgccgga cccgccgaag ccagacccca agccggtccc gccggttttc ctgccggacc
    10921 ttccgctggc tccctccccg cagctcaatc cgcagccgga accgccgagc ttgccggact
    10981 tggtcctgca ggcaccggtc aagtcgtagc atcaccgcta cccaggttgg gggacgatgt
    11041 catgaccgca gcatcctatt gggacatcgt ggggccgtcc ggaagcgatg cggagctcgc
    11101 gagagcatcc gcggccggcg accggacggc tttcgcggac atctacgacc gctacgccga
    11161 ccgcttgcac gatttctgcg tcggaatgct ggctgatcgg gacggcgcgg ccgactgcgt
    11221 gcaagacgtg ttctgtattg cggcgacgcg ccttccgcag ctgcgggatc cggataagtt
    11281 gcggccctgg ctctactcga tcgcccgcaa cgaggcgctg cggcgaatcc gtgagcgtcg
    11341 gcgcgaagca ccatccgaag agctgcccga tacggcctcg ggtgaacccg gtccggagac
    11401 cttggccgcg cgcaccgaac tcgccgatct gatcgccgac gccgcgggcg ggctttccga
    11461 ccgcgaccgc tccgtgctgg agctggcgtt ccggcacggt ttggatgggc ccgacctcgc
    11521 cgaggcgctg ggggtgtctg cctccaacgc gaacacgatc gtgcaccggc tgcgcgggac
    11581 catcgaacgt tcgcttggcg cactgttggt ttcgcgccgc gtccgaaaca gcggcggctg
    11641 cgcggaactg tcggcgatac tcgacggctg ggacggccac ttcaatgtgc ttatgcgcaa
    11701 acgcatttcg cgccacatcg agtcgtgtca gacatgcgac gaggagcggc gacggcttgt
    11761 gagcccagtt gcgctgctgg gcgccgcacc agcgttcatc cccgcgccgg catggcttcg
    11821 cgagcgcaca ctgagcgagg ttcagctgac gtccgccgca agtccgatcg gcgacggcgg
    11881 agccgatgcg ggccgtggta cccgcaactc tttgttgccg gtcacggtct tcgtcgtcgc
    11941 gttgctcgcc gcgctcgggt tgaccttcgt ttggatcaac ctgcaagaga cgacgacgat
    12001 ccagagcccg atcgaggtca gcgaaaccgc gccgcccccg gctgcgccca gcccggtggc
    12061 tccaccgccg ccgccaccac cgccgacggc cagcgtgaag ccaccccctc cggcaaccgc
    12121 accgcccccc cggcggacgc cgacgattga gcaaccgtcc gcggagccca cacccgttgc
    12181 accgccgccg atttcagagc cgacgccggt cgagcctccg cccccaccgc ccccaccgcc
    12241 accgccaccg ccctggcctc cgtggcccga gtggccacag tggcaatggc cagaccccca
    12301 aggcccgcgg ccaggcggtg gtgacctcac cgggccgggt gacttggtcc tgcccggcca
    12361 gggcgggtcg aatggttcgc ggccttgttc ggcaattcag tgcccgccgt gagccactat
    12421 ccggttcccg gcgccagcag taacctcaca ggttgcgcca aggttgaggg gctaacccag
    12481 tagggggcgt catgactgcg gtacccagtt gggatctggt ggacccgcgc cacacagacg
    12541 cggaactggt tggcgccagc atggccggtg accgagcggc gttcgcgcag atctatgacc
    12601 gctacgccga ccggctacac gacttctgcg tgggaatgct gcgtgatttc gacgctgccg
    12661 ccgactgcgt ccaggagacg ttctgtctcg ccgcgacccg ccttccccaa ttgagagagc
    12721 ccgacaaatt gcgcccatgg ctgtacgcga tcgcgcgcaa cgaatcgctg cgccggctgc
    12781 gggatcgcca gcgcgaaacg ccatctgacg aattgccgga catcgagtcc gacgatgcca
    12841 gccccgacac cctggctgca cgcaccgagc tcgccgattt gattgcagag gccgcggggg
    12901 gcttgacgga ccgcgaccgc gcggtctttg acttggcttt tcgccacggc ctgaacgggc
    12961 cggacctcgc cgatgcgctt ggtgtcagcc acaccaacgc caacacaatg gtgcagaggc
    13021 tcaggggaac gatcgaacgc tccctcggtg cattgttggt atgtcgccgc cgcaacccag
    13081 gcggatgcgc ggaactcggc cagatcctcg cgggctggga cggacagttc accgtgctga
    13141 tgcgcaaacg cgtgtcgcgg catatcgagt cgtgtccaaa ctgcgacgag gaacgacgcc
    13201 gattggtcag tcccgctgca ttgctcgctg ccacaccggc cttcattccc gcgccgaaat
    13261 ggctgcgcga caagacactc ggcgacatcc agctggtctg ccacgccagc gccatggatg
    13321 gcctatcaga cgatgaggcc gcctcccacc acgacgattc gggcgcatcc gatgttgacg
    13381 gcgccagcgg agagcaaggc cgcagacgag tgttgctggc cgccttgttg atcgccgtgt
    13441 tgggtaccac tgccggactg atgttggcgt ggctgaaccg cgaacccacc tcgattcttc
    13501 ctaccgagat cagtggaacg tcacccacga gccaggccac cttcccgatc agctcatcgc
    13561 caacccacac caccgcgccc gtgccgacca ccgagcccgt cgcccctctc cccccggccg
    13621 cggacttacc ccaggtgcct ggtacagcag ccacgcccga gctgattccg tccgagcctc
    13681 cgccttactc gccgccgtcc gaaccagcac cacggacacg gccgacggct attacgcgga
    13741 gcgcgccgtc gtattcgccg acacggacgg cggatccgac ttccccgggt ggcaatctcg
    13801 gcggaggaaa ccaaggcgga agcgatcgca atcccccgcc tggcggttct gctccgtagc
    13861 agacgtcgac ctggccgcga aaattctctg gccaaaaagt gtgccagtgg cgcgcggttg
    13921 gcgctcctac ctatgagccc aggagaggag cccatcatgc acatcaacaa cagccgcaag
    13981 aagttcgtca ccgctgcgtt cgccgcgatc gccggcgcgg tggctctgac gggctgccaa
    14041 tcgccgttca caagcgctga gccagattat gaagcgctgg agagttacgt cttcgatgag
    14101 gtcaacggcg actttccgtc gcagatctcg cgcgaggtgt caggtgtcga ttgtccgcgg
    14161 caggcggaca ccgtgacgtc tggtgagtcg ttcgtttgca ccgtggatgt cgacggtcat
    14221 gccgtgcgcg tggaagtgac ggcaaagaac gaagggctcg acttcaacac cctcgacatg
    14281 gtctacgacc tcggacccgc cgcgcaggac ttcgcggatc aaatttcgaa cgaggtcgag
    14341 tttcccatca ccctgagctg cggcgaaggc ctgaaggtgg ttccgatcgg agactcgttc
    14401 gagtgcaccg ccgccgatga gtacggcgac acccgcacca tcaggatcac ggccaacccg
    14461 gacggcgaat tctgggaggt tctcgactag cgctagccgg aagccccgac atcgcggtgt
    14521 agcgtgagat cgcctgcgtc tagggttccg ccgaccgccc gcctgtgatc gacattcagg
    14581 gccgccggga ctggtccgag cggcgccagg acaccttggt gatccacacc tgacgggata
    14641 aaagccctga gggctccttt aactgcgctc tgacacagag cccaacccga ggagccgcct
    14701 ggtgacaccc tccatcgtgc tcgccgtgtt gttcgcggcc gtgctgcacg cgatctggaa
    14761 ttcgttggcg catgccgtta gcgaccggct ggtgggcttc gcgttgatcg gattggtcga
    14821 cgcggtcggc ggcgcggccc tggcggcggc cggtggtctg ccgcctgcgg gagcgtggcc
    14881 gttcatcgtc gcctccgcgc tgacccacac ggcatacaac ctcttcctgc tggcgagcta
    14941 tcagctcggg gagttcagcc agatgtatcc gctggcccga ggcacatcgc cgtgggtggt
    15001 ggctctggta tcgatcgtgg tgttgcaccg agcgctgtcc atcaccgaac tggccggtgt
    15061 gcttgcggtg tcggccggac ttatcggtct ggtgctgatc ggtggtcggc ccgggtgcaa
    15121 ggatctgccg gcactgacgg cggcgctgct cactgggctg atgatttcga gctacaccgt
    15181 gatcgacggc gttggagtca cacatgcgcc tctgctggcc tacgcggggt ggatgttctt
    15241 gcttcaggcg cctccgatcg tgatgatcgc gctgatccga cgccgcggaa agttggtcgc
    15301 tgcgatgcgt gcctgcgcgt ttcccggtct gacgggcggc gccatctcat tggtcgcata
    15361 cacgatcgtg ttgtgggcgc aactcagcgg cgccctggcg acgatcgccg cgctgcggga
    15421 gacgagcatc gtgttcggcg ccctgatcgg gtcggtgttc cttggcgaac ggctcgggcc
    15481 acgccgtgcg atcgccgccg ccgtagtgct cggcggggtg gttctgatca gcctgccgtg
    15541 accatcaacc ggtgccgggc accagccagt ggcccgagaa ggcgcgccag ccgacgaacg
    15601 ccaggcgcaa caccatgaag gtgctcaagc ctgaccagat gccgagcaga ccccaaccga
    15661 atgccagcga cagccagatc aacggcagga atcccaccag cgcgctgatc aacgtcgcgt
    15721 tgcgcatgaa ctttgcgtcg ccggcgccga gcagcacccc gtcaagcgcg aaaacgattc
    15781 cagcgatggg caattgggcc accatgaacc accacggcac gccaatcgcg tcgagcacgg
    15841 aacggtcatt ggtgaacacg ctcggaaaca ccgacgcgcc gacggcgaag atgcccgcca
    15901 gcaccgcgct ggccaccgcg gaaaaaatcg tcacccgcca tgcgacggac ttggcatgcg
    15961 ccagttcacc agctcccaac gcggcgccca ccagcgactg cgccgcgata gccagcgaat
    16021 ccagcaccag cgcaaggaaa ttccacaact gcagcaccac ctggtgtgca gcgaccgcag
    16081 ccgcgccgaa ccgggcggcg acagcacctg ccgatacgaa gcacgcctga aacgccatcg
    16141 tgcgcagcac cagatcacga cccatcacga cctgtgcccg cagcaccgcc ggttggatcc
    16201 ccagtggcac gcgctcggcg aacaacgacc gcaagaacag cagagcggcc acccactgac
    16261 ccaccaggtt ggcgaccgcc gaaccgacca actccaaccg cggcatgccg agccagccgt
    16321 acaccagcag cggacacagc accgcggaca ccgcgaaccc ggctacgacg tatcgcagcg
    16381 gtcgcgctgt gtcctgcacg ccgcgcatcc agccgttgcc cgcggccgag accaggatcg
    16441 ccggcacgcc cacgatcgcg atgcgcaccc acggcaacgc ggcttccgca atgttcccac
    16501 ccgcggccaa cgccgacacc aacggcaccg caaagatctc gaccgcagcg acgatcagcg
    16561 cgcccaggcc gagggccaac caggtggcct gcaccccttc cccaaccgcg gccgcacggt
    16621 tgcccgcccc gaaaaaccgg gccgagcggg cagtggtgcc gtaggacaag aaggtcatgt
    16681 tcgaactgag cgtgcttaga atcagcccgc cgatagccag accggccagc ggcaacgcgc
    16741 ccaaccgtcc gacaacggcg atgtcgaaaa gcagatagat cggttcggcg gccagcacgc
    16801 caagcgccgg caaggccagg cccgcgatcc tgcgagtggt agccggggcg accggcagat
    16861 cgccctcggg ttcagccaag agccgcgtac agcgccgcca ccacgtcgtc ggccgatccc
    16921 gtcgccgaat agccggcggc cagcttgtgc ccgccgccgc cgaaggcact ggcaaccgcg
    16981 gcaaggtcgt aagacttcgc acgcatcgac accgaccagt gctgcggttc gatctccttg
    17041 aacaccgcgg ccacctcggc ctgctgggtg gtgcgcacga tgtcgacgat gctctcgact
    17101 tcctcgggcc tggcattggc ccattcctgg tgtcccacaa tggcatacac cagtccgcga
    17161 ccaccggcgg cgtcgggcag aagctgagcc gacgacagca cgcgcgacag catcggaagc
    17221 cacgcgaacg gatggctgtc aagcagcgtg cggctgatcg acgcattgtc gacgccgagt
    17281 tccaccagcc gggcggccag ccggtgcgcg cgggcgctgg cccaccggaa cgatccggtg
    17341 tccgtggtca gcccggcata caggcaatgc gcgacgccgg gctcgatcgg cttaccccat
    17401 gcgtcgagca gttccgcgac gagcatggtc gtggaatctg ccgacggatc aacgtaattg
    17461 gcggtgccga agagctggtt ggacgcgtgg tggtcgatca ccagcacctc gcggcccggg
    17521 tcggcgagct cactgagcgc gccaaggcgg ttgacgctgg ggatgtcgac ggtcacgacc
    17581 aggtcggcgt cgcgacgcat ttcgtccggg ccgaccaaca ggtggccacc gggcagcgac
    17641 tgcaatgact cgggcagctc ggccggcgcc gcgaaactga cctcgaccgc cttgccggca
    17701 tgatcaagca ccagcgccag cgccagccct gcgccaatgg tgtcggcgtc gggatagaca
    17761 tggcagacca cgctgacggt ggcagccgca tccagcaggt cggctacctg ttgcgcatcg
    17821 acgcgctcgc ccatgacagg gacgtcagtc gtctggtctg ttgccgtcac ccatgtcctc
    17881 agagtcgtct cgatccccgc cggactcctc caccccggta acacggtacg ggtccgcgtc
    17941 acctgcgtgc gtggcgccct cccgaactct cgccaaatcc tcatctgcgg cgcgcgcccg
    18001 tgccagcagt tcctccatcc ggtgcgcggt gtcgggaacg gtgtcgcgtt cgaacgccaa
    18061 tgtcggggtg aaccggacgc cgagcgccgc gcccaccttg gtccgcagca ctcccgtagc
    18121 gctctccagc gccgccgcca cgccggcgta gtccggctcg tcctcgagat tgcgaccgaa
    18181 caccgtgtaa aacagcgtgg cgtcgtgcag gtcgcccgtc atcttggcgt ccgtgatcgt
    18241 cacaccggcc agccgcggat ccttgatctc gtactcgatc gccgaggcga cgatggtcga
    18301 gatgcgcttg gccagccggc gtgctcgtgc cggatcagcc acgttccttc tcgaccagct
    18361 cgtacgtctc gatcacgtcg ccttccttga tatcggagta gctcagcgtc agaccgcatt
    18421 cgtagccgtc gcgcacctcg gtgacgtcgt ccttctcgcg ccgcagcgac tgcacggtgg
    18481 cgttctccgt gacaacgacg ttgtcgcgaa gcagccgtgc cttggcgttg cggcggatga
    18541 tgcccgactg gacgaggcag ccggcgatgt tgccgacctt cgagcttcgg aagatggcgc
    18601 ggatctcggc ccggcccagt tcccgctcct cgtagaccgg cttgagcatg cccttgagcg
    18661 cgctctcgat ctcgtcgatg gcctggtaga tcaccgagta gtagcggatt tccacaccct
    18721 cgcggttggc cagctcggtg gccttgccct cggcgcggac gttgaacccg atgatgatcg
    18781 cgtccgacgc cgacgccagg ttgacgttgg tctcggtgac accgccgacg ccgcggtcga
    18841 tgacgcgcag ctcgacctcg tcgtcgacct ggatgcccag cagggcctcc tcgagtgcct
    18901 cgacggtgcc tgcgttgtcg cccttgagga tcaggttcag ctggctggtt tccttcagcg
    18961 ccgaatccag atcttccagg ctgatccgct tgcgggtgcg ggcggccagt gcgttgcgct
    19021 tgcgcgcgct gcggcggtcg gcgatctggc gggcgatgcg gtcctcgtct acgacgagga
    19081 agttatcgcc agcacctggc accgacgtga agccgatgac ctgcaccggc cgcgacggca
    19141 gtgccgcctc gacgtcttcg ccgtgctcgt cgaccatgcg gcggacgcgg ccataggcgt
    19201 cgcctgccac caccgagtct ccgacgtgca gggtgccgcg ggtgatcagc acggtggcca
    19261 ccggcccgcg gccgcggtcc aggtgcgcct cgatcgcgac accctgggcc tccatatcgg
    19321 ggttggcccg cagatccagt gccgcatcgg cggtcagcac gaccgcctcg agcaaggact
    19381 cgatgttggt gccctgcttg gcggagatgt cgacgaacat cgtgtcgccg ccgaattcct
    19441 ctggcaccaa accatattcg gtcaactgcc cgcggatctt ctgcggatcg gcgccttcct
    19501 tgtcgatctt gttgactgcc accacaattg gcacctcggc ggcctgcgcg tggttgaccg
    19561 cctccaccgt ctgcggcatc acgccgtcgt cggcggcgac caccaggatc gcaatgtcag
    19621 tggccttcgc gccgcgggca cgcatggcgg tgaacgcctc gtgacccggg gtgtcgatga
    19681 aggtgatcgg ccggatggtg ccgtcgaggt cgacctcgac ctggtaggcg ccgatgtgct
    19741 gggtgatgcc gccggcctct tcctcgcgga cgttggcctg gcggatggtg tccagcagtc
    19801 gggtcttgcc gtggtcgacg tgacccatga ccgtgaccac cggcgggcgg gtctcgaggt
    19861 cttcctcgcc gccctcgtcc tcgccgtagg tgaggtcgaa ggactccagc agctcgcggt
    19921 cctcgtcctc gggcgagacg acctgaacgt tgtagttcat ctcgctgccc agcagctcaa
    19981 gggtctcgtc acccacggac tgggtggcgg tcaccatctc gccgaggttg aacagcgcct
    20041 gcaccagcga tgccgggttg gcgtcgatct tctcggcgaa gtcggacaag gaagcaccgc
    20101 gcgccaacct gatcgtctcg ccgttgccgt gcggcaaccg cacgccgccg acgaccggcg
    20161 cctgcatgtt ctcgtattcg gcgcgtttgg cccgcttcga cttgcgg
//
LOCUS       DASRJA010000615         7027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4044308,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000615 DASRJA010000000
VERSION     DASRJA010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4044308"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFC01_27695"
     CDS             complement(<1..478)
                     /locus_tag="VFC01_27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZN83436.1"
                     /translation="MTVDIRRADDRAKTKIDWLDSKHSFSFGSHYEADNTHHGLLLVN
                     NDDIVNPGTGFDTHPHRDMEIVTWVLRGSLVHQDSTGHSGVIYPGLAQRMSAGRGILH
                     SEKNDSWKLTGSETHDEPVHFVQMWVVPDESGITPGYQQLEIDDELLSGGLVTVASG"
     gene            556..990
                     /locus_tag="VFC01_27700"
     CDS             556..990
                     /locus_tag="VFC01_27700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZN83437.1"
                     /translation="MWLTEEQQQVWRGYLAMTSRLQTAMHRQLQQDCELSLADYDVLV
                     ALSEGGPQRINELGEVLAWEQSRLSHQLRRMRGRGLVERHGSDDDRRGATVDLSDAGR
                     AALEAAAPGHAELVRAVVFDGLSASQIRALAAWIERVTSRLR"
     gene            complement(971..2095)
                     /locus_tag="VFC01_27705"
     CDS             complement(971..2095)
                     /locus_tag="VFC01_27705"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016889453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HZN83438.1"
                     /translation="MKTKGALLWELNSPFRVDEIELGDPVADEVQIRMHAAGMCHSDY
                     HLTTGATPIGLPALGGHEGAGVVTKVGKNVTGIEEGDHVILAFIPACGECQPCLKGYR
                     SLCDRGALLLGGKAIADGTSRVHAGTTEVSPMNLLGTFAPYMTVHKDSVVKIDKDVPF
                     ETAAIMGCAVPTGFGSATNVAQVQPGETVVIVGVGGIGMSALQGAVISGAKHVIAIDP
                     NQWKRDQAIKFGATHAYPSMAEAIAPIIDLTYGVMADKTIIAVGEMKGEYVEEALTLT
                     TKTGTCVVTGMGSMMDVDVKLNLFLFTMLQKTLKGNIFGGGSSHVETPKLVGLYKSGL
                     LKIDEMVTNTYSLENINDGYQDMLDGKNIRGVVKFTEADW"
     gene            2353..3207
                     /locus_tag="VFC01_27710"
     CDS             2353..3207
                     /locus_tag="VFC01_27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83439.1"
                     /translation="MVTRRTLRSQYDPVYRNVYLPKGIQLTPATRAVAAWLWSGRQAT
                     VAGLSAAALYGTQWIDARRPAELYRRNGKPVDGIVIHRDELIDDETQLVSGIPTTTPG
                     RTAFDLGRRGRRTEALIAVDALANATRLRPPDVVPLLERHRGARGLVQLREVIDLMDC
                     GAESPQETRTRLLLIDAGLPRPTTQIIVYGNFAGKKYAKVDMGYEDFQVGVEYDGEQH
                     WTNPQQYAYDIDRHAELLARGWVIIRVSAEILRYRPDVIVVRVCAALQAAGAEWPVIA
                     RILGESVA"
     gene            complement(3223..4200)
                     /locus_tag="VFC01_27715"
     CDS             complement(3223..4200)
                     /locus_tag="VFC01_27715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN83440.1"
                     /translation="MTVLAHETDDEFLLKAEPHRRELLAHCYRMTGSLHDAEDLVQET
                     YLRAWKAYGGFKGQSSLRTWLYRIATNTCLTALESRQRRPLPTGLGQPSSDPVDDIAA
                     RDEVPWLEPLPDTSTDPTDPSEIVGTRESVRLAFVAALQHLSPRQRAVLVMRDVLQWK
                     AAEVGDAIGASTAAVNSLLQRARAQLEAVGPSEDDKLAEPDSPETKDLLDRYIAAFEN
                     YDIDKLVELFTADAVWEMPPFDGWYQGPHNIVLLSKTHCPAEGPGDMRFVLTTANGQP
                     AAALYMINRDTGAHEPFQMHVLAVAPDGISHVVAFHMTSFEKFGLPASL"
     gene            4261..5100
                     /locus_tag="VFC01_27720"
     CDS             4261..5100
                     /locus_tag="VFC01_27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83441.1"
                     /translation="MATTRKERSVDGVGGVRIVYDVWTPETEPRGVVVLAHGYAEHAR
                     RYDHVAQRFGESGLVTYALDHRGHGRSGGKRVYLRDISEYTDDFQTLVGIAAAEHPGL
                     KRVVLGHSMGGGIVFAYGVEHPADYDAMVLSGPAVDAQDNVSPAMVLLAKVLGKVAPG
                     LPVEQLPTDAVSRDPEVVAAYNADPMVFHGKLPAGIAKALIAVGETMPQRAGALTAPL
                     LVVHGEQDKLIHVDGSRHLVECVGSTDVHLKVYPELYHEVFNEPEQAVVLDDVTSWIE
                     VRL"
     gene            5097..6089
                     /locus_tag="VFC01_27725"
     CDS             5097..6089
                     /locus_tag="VFC01_27725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83442.1"
                     /translation="MKGLVLIAVSLILALAGCSSQDSSQEQHPWVDDDVSFDADGLTI
                     HGTYRHQKDSGPAPAALLISESGPTDRNGDNAVVGPIGNMKQLAEQLSDHGVASLRYD
                     KIGTGKTGLGPYRPNDVVSAVYTKGADAAVRFLADQAGTDKARISVYGLGEGTVHAMT
                     LATEATPGAPKIHSLGLFQPLPGRYLDIITSRVRGDASPQTLAAWLAAVDEIRTKGTV
                     PKDLPEGLGAIVNPGNVKAVVETDKIDPVVLAARLPAGTPVLLTCSDTDSQAKCDALK
                     PLIDALRHTALKVVELRGVNHVLHDDPSDSIANLTKPGPLSPQLVTALDAFVGQ"
     gene            6105..6854
                     /locus_tag="VFC01_27730"
     CDS             6105..6854
                     /locus_tag="VFC01_27730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HZN83443.1"
                     /translation="MSDDKMLARIAALLRQAEGTDNQHEADAFMAAAQRLATATSIDL
                     AVARAHSDNRSKAQTPVQRTITIGNAGARGLRTYVQLFTVIALANDVKCDVASNSTFV
                     YAYGFAEDIDASHALYASLLLQMVKASEAYISSGAHRPTPTITARINFQLAFGARIGK
                     RLVEAREQAQYEATRGRDSQPGTAVALRDKDIELKDFYRETSQARGTWRATSATAGYS
                     SAARRAGDRAGKRARLGGSPELAGARSALET"
     gene            6851..>7027
                     /locus_tag="VFC01_27735"
     CDS             6851..>7027
                     /locus_tag="VFC01_27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04338 family metallohydrolase"
                     /protein_id="HZN83444.1"
                     /translation="MTPRDTQRAKVYAAEELVRTLFDRAAEHGNNVVEFFGTQLTLPP
                     EGRFATVTAVQRYVD"
BASE COUNT         1252 a         2286 c         2311 g         1178 t
ORIGIN      
        1 tgcctgaggc gacggtgacc aggccgccgc ttaacaactc gtcgtcgatc tcgagctgtt
       61 gatagccggg cgtgatgcct gattcgtcgg gcaccaccca catctgcacg aaatgcactg
      121 gctcgtcgtg agtttcggag ccggtcagct tccacgagtc gttcttctcc gaatgcagga
      181 tgccgcggcc ggcggacatg cgctgggcca ggccggggta gatcacgccg gagtgcccgg
      241 tggagtcctg gtggacaagc gagccacgca gcacccacgt cacgatctcc atgtcgcggt
      301 gggggtgggt atcaaacccc gtgccggggt tgacgatgtc gtcgttgttc accagcagca
      361 ggccgtggtg ggtgttgtcg gcctcgtagt ggctgccgaa cgagaacgag tgcttggagt
      421 ccagccagtc gatcttggtc ttcgcgcgat cgtcggcgcg gcggatgtcg acggtcatgt
      481 cctcactcct cggttctggc cagcctaggt ggatggcgtt gaccgcaaca acatagatga
      541 tgtgtcatat attccatgtg gttgaccgag gagcagcagc aggtatggcg gggatacctg
      601 gcaatgacga gccggctgca gaccgccatg catcgccagc tgcagcagga ctgcgaactg
      661 tcgctcgccg actacgacgt gttggtcgcg ttgtcggaag gcgggccgca gcggatcaac
      721 gagctcggtg aagtgctggc ctgggaacag agcaggctgt cacaccaact gaggcgaatg
      781 cggggccgcg gtttggtcga acgccacggc agcgacgacg accgtcgcgg cgcgaccgtc
      841 gacctcagcg atgccggccg tgcagccctc gaggccgcgg cgccgggcca cgccgaactc
      901 gtgcgcgctg tcgtgttcga cgggttgagc gcctcgcaga tacgcgcgct ggcggcgtgg
      961 atcgagcggg ttaccagtcg gcttcggtga acttgacgac gccgcggatg ttcttcccgt
     1021 ccagcatgtc ctggtagccg tcgttgatgt tctccaacga ataggtgttg gtgaccatct
     1081 cgtcgatctt gagcaaccct gacttgtaga gcccgacaag tttcggcgtc tcgacgtgac
     1141 tgctgccccc gccaaagatg ttgcccttca acgtcttctg caacatcgtg aacaggaaca
     1201 ggttgagctt tacgtcgacg tccatcatcg agcccatgcc ggtcaccacg caggtgccgg
     1261 tcttggtggt cagcgtcaac gcttcttcga cgtactcacc cttcatctca ccgactgcga
     1321 tgatggtctt gtcggccatc acgccgtagg tcaggtcgat gatgggcgcg atggcctcgg
     1381 ccatcgacgg gtaggcgtgg gtggcgccga acttgatcgc ctggtcgcgc ttccactggt
     1441 tgggatcgat cgcgatgacg tgcttggcgc cggagatgac cgcgccctgc agcgcgctca
     1501 tgccgatgcc gccgacgccg acgatcacga cggtctcgcc gggctgcacc tgggcgacgt
     1561 tggtggccga cccgaagccg gtcggcaccg cgcagcccat gatggcggcg gtctcgaacg
     1621 ggacgtcctt gtcgatcttg acgaccgaat ccttgtgcac cgtcatgtac ggggcgaagg
     1681 tgccgagcag gttcatcggt gacacctcgg tggtgccggc gtgcacgcgg ctggtgccgt
     1741 cggcgatggc cttgccgccg agcagcaggg cgccgcggtc gcacaacgag cggtaaccct
     1801 tcagacacgg ctgacactcg ccgcacgcgg ggatgaacgc aaggatgacg tggtcgccct
     1861 cttcgatgcc ggtgacgttc ttgccgacct tggtgacgac gcccgcgccc tcgtggccgc
     1921 cgagcgcagg caggccgatc ggggtggctc cggtagtcag gtggtaatcc gagtggcaca
     1981 tgcctgcggc gtgcatccgg atctgcactt cgtcggcgac tgggtcgccc agctcaatct
     2041 cgtcgacccg aaacggggaa ttgagctccc acagcagagc gcctttggtc ttcatgaatc
     2101 gcgatcatag aacacgttcc aaaacacgtg tcaagaagcc cgttgacctg gggttagctc
     2161 cagctagaca acgtcgtcgc cgaacggcca cttatgccac gttttgcgcg ctgaggcgtg
     2221 cggtaactag ccactcgggc gacggaccct gtggacaacc cccgctgagg aagcgaaacc
     2281 gtcgcacctg atcgagacgg tgggcgcgtg aacacgacgc cgtttctcgg cactgaagcg
     2341 cttgcacgcg gcatggtcac caggcgaacg ctgcgtagcc aatacgatcc ggtatatcgc
     2401 aacgtctacc tgcccaaagg tatacagctg acgccggcta cccgcgccgt cgcggcatgg
     2461 ctatggtcgg gccgacaagc gaccgtcgca ggtctgtcgg ctgcggccct gtacggcact
     2521 cagtggatcg atgcgcgacg gccggcggag ttgtaccgcc gcaacggcaa gccggtcgac
     2581 ggcatcgtca tccatcggga cgagctgatc gacgacgaga cgcaactggt cagcggcatc
     2641 cccacgacga cgcccggccg tacggcgttc gatctaggcc gccgtggccg tcgcaccgag
     2701 gcgctcattg cggttgacgc attagcgaac gcgacgaggc ttcggccccc tgatgtcgta
     2761 cctctgttgg agcgacaccg cggagcgcga ggtctggttc aactacgcga agtgatcgac
     2821 ctaatggact gcggcgccga atcgccgcag gagacccgta cacggctgct gctcatcgac
     2881 gcgggactgc cgcgaccaac gacgcagatc atcgtctacg gcaacttcgc cgggaagaag
     2941 tacgcgaaag tcgacatggg ctatgaggac ttccaagtcg gtgtggagta cgacggcgag
     3001 caacattgga ccaacccgca gcaatatgcg tacgacatcg accggcacgc ggagttgctg
     3061 gcccgcgggt gggtgatcat ccgcgtgagc gccgagatac tccggtatcg accggacgta
     3121 attgtggtgc gagtgtgtgc tgcactgcag gcggctggcg ctgaatggcc agttattgca
     3181 cgaattttgg gcgaaagcgt ggcataagtg gccgctcgac gcctacagcg aggccggcag
     3241 gccgaatttc tcgaacgacg tcatgtggaa cgccacgaca tgggatatgc cgtccggggc
     3301 gaccgcgagt acatgcatct ggaacggctc gtgcgcgccg gtatcccggt tgatcatgta
     3361 gagcgccgcg gcgggctggc cattggcggt cgtgagcacg aaccgcatat cgcccgggcc
     3421 ttcggcaggg caatgcgtct tcgaaagcag gacgatgttg tgcgggccct ggtaccagcc
     3481 gtcgaacggt ggcatctccc aaaccgcgtc agcggtgaac agctcgacca gcttgtcgat
     3541 gtcgtagttc tcgaatgccg cgatgtagcg gtcgagcaag tccttggtct ccggcgaatc
     3601 gggctcggcc agcttgtcgt cttcactggg gccgacggcc tcgagctgcg cccgcgctcg
     3661 ctgcaagaga ctgttgaccg ctgcggtcga cgcgccgatg gcgtcgccca cttcggcagc
     3721 cttccactgc agcacgtcgc gcatcaccag caccgcccgc tgccgcggcg acagatgctg
     3781 caacgccgcc acgaacgcca accgcaccga ctcgcgtgta ccgacgatct cggaagggtc
     3841 agttggatcg gtcgaagtgt caggcagcgg ctccaaccac gggacctcgt cacgcgcggc
     3901 gatgtcgtcg accggatccg aactcggctg gcccagaccc gtaggcaagg ggcggcgctg
     3961 tcgcgattcc agcgctgtca ggcacgtgtt ggtggcgatc cggtacagcc aggtccgcaa
     4021 ggacgactgg cccttgaagc cgccataagc cttccatgcc cgcaggtagg tttcctgaac
     4081 caggtcttcg gcgtcgtgca gagaaccggt catccggtag cagtgcgcca acagttcgcg
     4141 ccggtgaggc tcggccttga gcaggaactc gtcgtcggtt tcgtgagcga ggacggtcac
     4201 ctagccgagc ttacgcagcc ccagagacaa ggcgagccaa gcactcatta atctcccctg
     4261 atggccacaa cccgcaagga acgcagcgtc gacggggtcg gcggcgtgcg catcgtctac
     4321 gacgtatgga cgccggaaac cgaaccacgc ggcgtcgtcg tgctcgcgca cgggtacgcc
     4381 gagcatgccc gccgctacga ccatgtcgcg cagcgattcg gcgaatccgg cctggtcacc
     4441 tatgcgctgg accatcgcgg acacggccga tccggcggca agcgggtcta tctgcgcgac
     4501 atctccgaat acaccgacga cttccagacc ctggtcggca tcgcggccgc cgagcacccc
     4561 gggctgaagc gcgtcgtgct gggccacagc atgggtggcg ggatcgtctt cgcctacggc
     4621 gtcgagcacc ccgcagacta cgacgcgatg gtgctgtccg gcccggccgt cgacgcgcag
     4681 gacaacgtgt caccggcgat ggtgttgctc gccaaggtgc tcggcaaggt cgcgcccggc
     4741 ctgccggtcg aacagctgcc caccgacgcg gtgtcccgcg accccgaggt cgtcgccgcc
     4801 tacaacgccg acccgatggt cttccacggc aagctgcccg ccggcatcgc caaggcattg
     4861 atcgcggtgg gcgagacgat gccgcagcgg gccggggcgc tgaccgcgcc gctgctcgtc
     4921 gtgcacggcg aacaggacaa gctgatccac gtcgacggca gcaggcacct ggtcgaatgc
     4981 gtcggttcca ccgatgtgca cctgaaggtc tacccggagc tctaccacga ggtgttcaac
     5041 gagcccgaac aagccgtggt gctcgacgac gtcacgtcgt ggatcgaggt tcgcctttga
     5101 aaggccttgt tctcattgcc gtttcgctga tcctggcgct ggctggctgc tcgtcgcagg
     5161 actcgtcgca ggagcagcat ccctgggtcg acgacgacgt cagcttcgat gccgacggcc
     5221 tgaccatcca cggtacgtac cggcatcaga aggactccgg acccgcgccc gcggcgctgc
     5281 tgatctccga aagcggaccg accgatcgca acggagacaa cgcggtcgtc ggtcccatcg
     5341 gcaacatgaa gcaactcgcc gaacagcttt cggaccacgg cgtcgccagc ctgcgctacg
     5401 acaagatcgg caccggcaag accggcctcg gtccataccg accgaacgat gtcgtcagcg
     5461 ccgtctacac caagggtgcc gacgcagcgg ttcgtttcct cgccgatcag gccggcaccg
     5521 acaaggcccg catctcggtg tacggcctcg gtgaaggcac cgttcacgcg atgacgctcg
     5581 cgaccgaagc gacgccgggc gcgccaaaga tccactcgct gggcctgttt cagccgctgc
     5641 cgggccgcta cctcgacatc atcaccagca gggtgcgcgg cgatgcaagc ccccagacac
     5701 tcgcggcgtg gctggccgct gtcgatgaaa tccggaccaa ggggactgtg cccaaggatc
     5761 tgccggaggg gctgggcgcg atcgtcaacc ccggcaacgt gaaggccgtt gtcgagaccg
     5821 acaagatcga cccggtcgtg ctggccgcga gactgcccgc aggcacgccg gtcttgttga
     5881 cgtgctcgga cactgacagc caggccaagt gcgacgccct gaagccgctg atcgacgctc
     5941 tcagacacac cgcgctcaag gtggtcgaac tgcgcggcgt caaccatgtg ctgcacgacg
     6001 accccagcga cagcatcgcc aacctcacca agccgggtcc gctttctcca caactcgtga
     6061 ccgcgctcga cgcctttgtc ggccagtagc cctaatctgg cgtcatgagt gacgacaaga
     6121 tgctcgcccg catcgccgcg ctgctccgcc aggccgaggg caccgacaac cagcacgagg
     6181 ccgatgcgtt catggccgcc gcacagcggc tggccacagc gacgtcgatc gatctcgcgg
     6241 tggcccgcgc acactcggac aaccgctcca aagcgcagac gccggtgcag cggaccatca
     6301 ccatcggcaa tgccggcgcc cgcggcctgc gcacgtatgt gcagctgttc acggtgatcg
     6361 cgctcgccaa cgacgtcaag tgtgacgtcg catcgaattc gacgtttgtc tacgcgtacg
     6421 gcttcgccga ggacatcgac gccagtcacg cgctgtatgc cagcctgctg ctgcagatgg
     6481 tcaaggcgtc ggaggcctac atctcgtcgg gtgcgcaccg cccgacgccg acgatcaccg
     6541 cgcggatcaa cttccagctg gcgtttggcg cccgcatcgg taagcgactg gtcgaggcgc
     6601 gtgagcaggc gcagtacgag gcgaccaggg gccgcgacag ccagccgggt accgctgtcg
     6661 cgttgcggga caaggacatc gaactgaagg atttctaccg cgagacgtcg caggcgcgcg
     6721 gtacgtggcg ggccaccagc gcgacggccg gctactcgtc ggcggcccga cgcgcgggtg
     6781 acagagccgg caagcgggcc cggctgggcg gcagccccga actggcgggt gcccgttcgg
     6841 cattggagac gtgacgccgc gcgacacgca gcgcgcaaag gtgtatgccg ctgaggaatt
     6901 ggtgcggacg ctgttcgacc gcgcggccga gcacggcaac aacgtcgtcg agttcttcgg
     6961 cacgcagctg acgctgccac ctgaggggcg gttcgcgacg gtgacggcgg ttcagcgcta
     7021 cgtcgac
//
LOCUS       DASRJA010000616         5011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_9265, whole
            genome shotgun sequence.
ACCESSION   DASRJA010000616 DASRJA010000000
VERSION     DASRJA010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9265"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFC01_27740"
     CDS             complement(<1..445)
                     /locus_tag="VFC01_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN83445.1"
                     /translation="MPRVTDDHLAARRRQILDGARRCFAEYGYDRATVRRLEQTIGLS
                     RGAIFHHFRDKDTLFFELAREDAERMADVASREGLIQVMRDMLAAPDQFDWLATRLEI
                     ARKLRNDPVFNRGWSERSAELSAATTQRLRTQKQAGRLRDDVPSAV"
     gene            complement(456..3260)
                     /gene="acnA"
                     /locus_tag="VFC01_27745"
     CDS             complement(456..3260)
                     /gene="acnA"
                     /locus_tag="VFC01_27745"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HZN83446.1"
                     /translation="MSSKDSVNSFGARDTLKVGDKSYEIYRLDAVSGTDKLPYSLKVL
                     AENLLRTEDDTNITKDHIEAIANWDPEADPSVEIQFTPARVIMQDFTGVPCIVDLATM
                     REAVGDLGGDPDKVNPLAPADLVIDHSVIADLFGTANAFERNVEIEYERNGERYQFLR
                     WGQGAFNDFKVVPPGTGIVHQVNIEYLASVVMERDGVAYPDTCVGTDSHTTMENGLGV
                     LGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLSGERPTGVTATDVVLTVTEMLRKHGVV
                     GKFVEFYGEGVAEVPLANRATLGNMSPEFGSTAAIFPIDEVTIDYLRMTGRSEEQLAL
                     VEAYAKEQGMWHDPKREPKFSEYIELDLSDVVPSIAGPKRPQDRIALSDAKSAFRKDI
                     HNYVEENMPVEHSKLDEAVEESFPASDPAVLSFDDNDVVEPSAAVGANGRPSKPVEVK
                     SDERGEFVLDHGAVAIAAITSCTNTSNPEVMLGAALLAKNAVEKGLTSKPWVKTTMAP
                     GSQVVSDYYDKAGLWPYLEKLGFYLVGYGCTTCIGNSGPLSDEISKAINDNDLSVAAV
                     LSGNRNFEGRINPDVKMNYLASPPLVIAYALAGTMDFDFENDCLGTDKDGNEVFLKDI
                     WPSQKDITDTIASAINTEMFTKNYADVFKGDDRWRNLPTPSGNTFEWAEDSTYVRKPP
                     YFDGMPAEPEPVTDIQGARVLALLGDSVTTDHISPAGSIKPGTPAAQYLDEHGVDKKD
                     YNSYGSRRGNHEVMIRGTFANIRLRNQLLDDVSGGYTRDFTNGGEQAFIYDAAQNYAA
                     QDIPLVVLGGKEYGSGSSRDWAAKGTRLLGVRAVITESFERIHRSNLIGMGVIPLQFP
                     EGESASSLKLDGTETFDITGIEALNDGKTPKTVHVTATKEDGKKVEFDAVVRIDTPGE
                     ADYYRNGGILQYVLRNMLKT"
     gene            3422..3961
                     /locus_tag="VFC01_27750"
     CDS             3422..3961
                     /locus_tag="VFC01_27750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6676 family protein"
                     /protein_id="HZN83447.1"
                     /translation="MTGPHVIPFLPSYVPPDVDMNQVRADVADDGVSVPPADAADVPA
                     LRQVVEEARKDGIDLKIVVIDRNPPIDTPLRDIATEVGKTYPEATVLAISPSFAGTYS
                     GHFDRVTLEAGQDVAKTGNSVQSSKNFLSELTTPHFPWTPFTIVVVLAVIAAVVTTRV
                     LRVSGKRAAAAEAASDTAD"
     gene            4177..>5011
                     /locus_tag="VFC01_27755"
     CDS             4177..>5011
                     /locus_tag="VFC01_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HZN83448.1"
                     /translation="MRRTPRVRAVTLAIGMLLATPGLAVAQPGSADTLGALVAAVANA
                     NQKLQELGAAIQAQQESVNKAIVDVQTARDNAAAAQQEVEASAARVVDANTAIAGAQQ
                     KFDTFAAATYVNGPSDSYLTASDPADILSTAAAGQTLSANSQQVIINLQRARTEQVNK
                     ESAARLAKQNADKAVADAEASQQTAVSALTQVQQTFGAQQAELDKLTAERAAAQAKLD
                     EARNWSAPAPGQPAAQPAAARVSDPNANWDRAPGARDAGAGNWDSLWDPTLPAIPSAF
                     VS"
BASE COUNT          845 a         1613 c         1621 g          932 t
ORIGIN      
        1 gcaccgcgct gggcacatcg tcgcgtagcc ggcctgcctg cttctgggtg cgcagacgtt
       61 gggtggttgc cgccgacaac tccgccgagc gttcggacca accgcggttg aacaccggat
      121 cgttgcgcag cttgcgcgcg atctccagcc gggtcgccag ccagtcgaac tggtcgggag
      181 cggccagcat gtcgcgcatc acctggatca gaccttcacg cgacgcgacg tcggccattc
      241 gttcggcgtc ctcgcgcgcc agctcgaaga acaacgtgtc cttgtcgcgg aagtgatgaa
      301 agatcgcacc gcgcgacagc ccgatcgtct gctcgagccg ccgcacggtg gccctgtcat
      361 atccatattc ggcgaagcac cgccgggcgc cgtcgagaat ctggcgccgg cgcgcggcca
      421 gatggtcgtc ggttacccgg ggcatgtcta agtacttacg tcttcaacat gttccgcagc
      481 acgtactgca ggatgccgcc gttgcggtag tagtccgcct caccgggggt gtcgatgcgc
      541 accacagcgt cgaactcgac cttctttccg tcttccttcg tggccgtgac gtgcaccgtc
      601 ttcggcgtct tgccgtcgtt gagcgcctcg atgccggtga tgtcgaacgt ctcggtgccg
      661 tccagtttga ggctcgacgc agattcccct tcggggaact gcaacgggat gacgcccatg
      721 ccgatcaggt tggagcggtg gatgcgctcg aacgactcgg tgatgaccgc acgcaccccg
      781 agcagccggg tgcccttggc cgcccagtcg cgcgaggaac ccgagccgta ctccttgccg
      841 cccagaacga cgagcggaat gtcctgcgcc gcatagtttt gcgcagcgtc gtagatgaac
      901 gcctgctcgc cgccgttggt gaagtcgcgg gtgtagccgc cggacacgtc atcaagaagt
      961 tgattacgca accggatgtt ggcgaacgtg ccgcggatca tcacctcgtg gttgccgcgc
     1021 cgtgagccgt aggagttgta gtccttcttg tccacaccgt gctcgtcgag gtactgggcg
     1081 gccggggtgc ccggcttgat gctgcctgcg ggggagatgt ggtcagtggt caccgaatca
     1141 cccagcagcg cgagcactct ggcgccctgg atgtcggtca ccggctcggg ctcggccggc
     1201 atgccgtcga agtacggggg cttgcgcacg tacgtcgaat cctcggccca ctcgaaggtg
     1261 ttgccgctcg gggtcggcag gttgcgccag cggtcgtcac ccttgaacac gtcggcgtag
     1321 ttcttggtga acatctccgt gttgatcgcc gaggcgatgg tgtcagtgat gtccttctgc
     1381 gacggccaga tgtccttcaa gaacacctcg ttaccgtcct tgtcggtgcc aaggcagtcg
     1441 ttctcgaagt cgaagtccat ggtcccggcc agcgcgtagg cgataaccag cggcggcgac
     1501 gcgaggtaat tcatcttcac gtcggggttg atgcggccct cgaagttgcg attgcccgac
     1561 agcaccgctg caaccgacag atcgttgtcg ttgatggcct ttgagatctc atccgacagg
     1621 gggccggagt tgccgatgca cgtcgtgcag ccgtagccga cgagatagaa acccagcttt
     1681 tccaggtacg gccacaggcc ggctttgtcg tagtagtcgc tgacgacctg cgagccgggc
     1741 gccatcgtcg tcttgaccca cggcttggac gtcagaccct tttcgaccgc gttcttggcc
     1801 agcagcgccg cgccgagcat cacctcgggg ttggaggtgt tcgtgcagga ggtgatcgcc
     1861 gcgatcgcca ccgctccgtg atcgaggacg aactcgccac gctcgtcgga cttcacctcc
     1921 accggcttgc tcggtcgccc gttggcgccg acggccgccg acggctcgac gacgtcgttg
     1981 tcatcgaacg acagcacggc cgggtcgctg gcaggaaagc tttcctcaac cgcctcgtcg
     2041 agcttggaat gctcgaccgg catgttctcc tcgacgtagt tgtgaatgtc cttgcggaac
     2101 gccgacttcg cgtcggacag tgcaatgcgg tcctgcggac gcttgggtcc ggcgatcgac
     2161 gggacgacat cggacaggtc cagctcgatg tactccgaaa acttcggctc gcgcttgggg
     2221 tcgtgccaca tgccctgttc cttggcgtag gcctcgacca gcgcgagctg ctcctcgcta
     2281 cggccggtca tccgcaggta gtcgatggtg acctcgtcga tcgggaaaat cgctgcggtg
     2341 gagccgaatt cggggctcat gttgcccagc gttgcgcggt tggccagcgg cacctcggcc
     2401 acaccctcgc cgtagaactc gacgaacttg ccgacgacgc cgtgcttgcg cagcatctcg
     2461 gtgacggtaa gcaccacgtc ggtggcagtg acgccggtcg ggcgctcgcc ggagagtttg
     2521 aatccgacca cccgcgggat cagcattgac accggctggc cgagcatggc ggcctcggcc
     2581 tcgatgccgc cgacgcccca gcccagcacg ccgagtccgt tttccatggt ggtgtgcgag
     2641 tcggtgccga cgcacgtgtc ggggtaggcg acgccgtcgc gctccatcac cacgctggcc
     2701 aggtactcga tgttgacctg gtgcacgatg ccggtgccgg gcggtaccac cttgaagtcg
     2761 ttgaacgcgc cctggcccca tcgcaagaac tggtagcgct cgccgttgcg ctcgtattcg
     2821 atctcgacat tgcgctcgaa cgcattcgcg gtcccgaaga ggtcggcgat caccgagtgg
     2881 tcgatgacca ggtcggcggg cgccagcggg ttgaccttgt cggggtcgcc gcccaggtcg
     2941 ccgaccgcct cgcgcatggt ggccaggtcg acgatgcagg gcaccccggt gaagtcctgc
     3001 atgatcaccc gagccggggt gaactggatc tccacgctcg ggtcggcctc gggatcccag
     3061 ttcgcgatgg cctcgatgtg gtccttggtg atgttggtgt cgtcctcggt gcggagcaga
     3121 ttttcggcga gcaccttgag gctgtaggga agtttgtcgg tgccggacac cgcgtccagg
     3181 cggtagatct catagctctt gtccccgacc ttgagcgtgt cgcgggcccc gaaggaattc
     3241 accgaatctt tgctgctcac atcaactccc ggctcgatat cgccgcgacg ggctgtgtcg
     3301 gcggcgctaa caactctaac agtacgcttg tcctgcaata caccccaggg gtgctttcag
     3361 tcttgtcctc aggagcgcgt gctgccaccc ggttgggtac ccatcacgta cggtgctctt
     3421 cgtgaccgga ccgcacgtga tcccgtttct gccttcgtac gtcccgcccg atgtcgatat
     3481 gaatcaggta agggccgacg tggccgacga cggagtgagc gtgccccccg ccgacgcggc
     3541 cgacgtgccg gcgctgcgtc aggtcgtcga ggaagcgcgc aaggacggca tcgacctcaa
     3601 gatcgtcgtc atcgaccgca acccgcccat cgataccccc ctgcgcgaca tcgccacaga
     3661 ggtcggcaag acgtatccgg aagccaccgt gctcgctatt agtccgtcgt tcgccggcac
     3721 ctacagcgga cacttcgaca gggtgacgct cgaggcgggc caggatgtcg ccaagacggg
     3781 caattcggtg cagtcgtcga agaatttcct cagtgagttg acgacgccgc actttccgtg
     3841 gacgccattc acgattgtgg tcgtgctggc ggtgattgcg gcggtcgtca ccacccgcgt
     3901 gcttcgggtt agcggcaaac gcgctgccgc ggcagaggcc gcgtccgaca ccgctgattg
     3961 accaatattc ggcattcggc aaatcaaata gtcacagcgg gataacgcta cattcaatta
     4021 cagacctgta atttgtgact aaagtttcct tagcgtcaga tgtgacgtac cgtgcaatcg
     4081 gtggctgatg ttgcagttgt gatttttgtg actgttcata ggcgcccacg aatgtgccgc
     4141 cacgttcgcg tgtgagccat aggagacacg agccgaatga gacgcacccc tcgggtccgc
     4201 gcggtgacgc ttgccatcgg catgctgctt gccacgcccg gccttgccgt cgcgcaaccg
     4261 ggcagcgcgg acactctcgg cgcgctcgtc gctgcggttg ccaacgcgaa ccagaagctg
     4321 caagaacttg gcgctgccat ccaggcccag caggaaagcg tcaacaaggc catcgtcgac
     4381 gtgcagacgg cgcgggacaa cgccgccgcc gcacaacagg aagtggaggc cagcgctgcg
     4441 cgggtcgttg acgcgaacac ggcgatcgcc ggggcgcagc agaagttcga taccttcgct
     4501 gccgcaacgt atgtcaacgg gccgtcggat tcgtatctga ccgccagcga tcccgctgac
     4561 atcctgtcga ctgcggcggc cgggcaaaca ctgtcggcga attcgcagca ggtgatcatc
     4621 aacctccagc gcgcgcgcac cgagcaggtg aacaaggaat cggccgcccg gcttgcgaag
     4681 cagaacgcgg acaaggccgt cgccgacgcg gaagccagcc agcagaccgc tgtgtcggcg
     4741 ctgacgcagg tgcagcagac gttcggtgca cagcaggccg agctggacaa gttgacggcc
     4801 gaacgcgcgg ccgcacaggc caagctcgat gaggcgcgca actggtcggc acccgcacca
     4861 ggtcagccgg ccgcccagcc cgccgcggcg cgcgtcagcg acccgaatgc gaattgggat
     4921 cgcgcaccgg gtgcgcgcga tgcgggcgcc ggaaattggg actcgctgtg ggatcccacg
     4981 ctgccggcga tcccgagcgc gttcgtcagc g
//
LOCUS       DASRJA010000617        11386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2612460,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000617 DASRJA010000000
VERSION     DASRJA010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11386
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2612460"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VFC01_27760"
     CDS             <1..551
                     /locus_tag="VFC01_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZN83449.1"
                     /translation="IALGWWPLAALFLALRLAGANLDGAVARARGVSRPWGFVVNEIG
                     DRTSDLLTFAGLAVFAARQHGPGFQWLSWSVLQVLVAALAATLPTFASLAAAAAGATR
                     RNGGPLGKTERCLFVLLATAFPVIMPIVLTQLVNGSILTTVLRLRAARRELAEKRQQE
                     QERAEVAEQTADTRPILSVVAA"
     gene            548..1177
                     /locus_tag="VFC01_27765"
     CDS             548..1177
                     /locus_tag="VFC01_27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN83450.1"
                     /translation="MNAISYVLRHWMWRLVSALSGGLTVSGKWLASGGCIVVANHSSH
                     ADTAVLLAALPPKAKPVFGAAADYWFDVPVRRFIATSLAGVLPVRRSGGGSYDALLAA
                     ARPALKAGRTVVIYPEGTRSTDGTIGEFRSGALRLARECGVPIVPVAMTGTADVLPKG
                     GRWSPAPMQVRIGEPVDPTTASAAELREHVLALRDGLQPDVVRRYALAA"
     gene            complement(1184..3553)
                     /locus_tag="VFC01_27770"
     CDS             complement(1184..3553)
                     /locus_tag="VFC01_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN83451.1"
                     /translation="MSRPLPPTITVYAGGWHRTFGPGGDVVIGRDVHADVRLPQPMVS
                     RAHVVLCYLDHHWVAIDNSSTNGMFVDRERVSSVNIRDGQAIHIGDPNGPRVTFGLGH
                     DGESADFPPDDRTTIPGKRDADVKTDVVDIVTTLIRFLRPGVPAAAPPGSTTIGREVD
                     NDIVIGDLLASRHHARLVPTAQGMQIQDISSNGTFVNGQRVKDAYLSENDVVTIANVD
                     MVFANGTLVRRTEPAAKSGGLDVCDVSLTIGGNHTLLDRVSFSAKPATLTAVIGPSGS
                     GKSSLLNVMVGGARPTGGAVSFDGHNIHAEYASLRNRIGMVPQDDVVHRQLTVNQALG
                     YAAELRMSPDTTKEERQWVVRQVLEELEMTPHAETRIDKLSGGQRKRASVGLELLTGP
                     SLLVLDEPTTGLDPALDQVVMKMLRQLADAGRIVVVVTHSLAYLDVCDQVLLLAPGGK
                     TAFCGPPSELGPAMGTTDWADIFTHIGADPDAAHRRFLERAEHPVGSAGPAAPEHLAA
                     LGKPVRTGLWRQFSTIARRQVRLIRADRGYFMFLVLMPFLLGLLPLAVAGDTGFGKAA
                     LESASPNEPKQIVVLLNLGAIFMGTALTIRDLVGERVIFRREQTAGLSASAYLAAKIA
                     VFGTAAVVQSAILVLIVTAPTIGKGAPLGAVVLGAPRLELFVDIAATCVAAAIVGLFV
                     SALAQNSNQVLPLMVVTVMTQLVLAGGFIPVTNRPLDPVSWLTPARWGLAATASTVDL
                     TNTVAVIPHDSHWKHTASAWTFDIVMLGVLSVCYASVVRWKIRLASTMK"
     gene            complement(3576..4169)
                     /locus_tag="VFC01_27775"
     CDS             complement(3576..4169)
                     /locus_tag="VFC01_27775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83452.1"
                     /translation="MDDRDDMQERRSRVWSAIARVIGGPYGLPLAFLWGFAEALSWFI
                     VAEMALLLFAAAVPRRVMPCAAAVIGGSVLGVLTNAWLASRGVLLPAPLTTTRMAATA
                     FDQFSAGPSAIMNQALSGIPVKVYARAAGEHHIDLWQLAGWTLLERGLRISTVGLAVW
                     LLSKLLHPLLRRLYGVYMIIVCVLFTAGLSAVIAAWS"
     gene            complement(4212..5117)
                     /locus_tag="VFC01_27780"
     CDS             complement(4212..5117)
                     /locus_tag="VFC01_27780"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZN83453.1"
                     /translation="MLIDWYLSIVHFDRRMHLNLDLGPLHIHLYARAVFFMWVTVAIL
                     VIGGIAVFASRKRELIQKWTTWVLIAPIVGIPVWMGRGPTAALAAALAVVAVMEYARL
                     VRLSRVDTGILLALGVLYPLAAWLRPSLLGLAPIIVLACALPSVLGGDVEHGGRRTAF
                     TAFGSVWICWSLAHLVMVWPDTFLVAFAASAADVAAWCGGKGLRRFGWARKPLSPLSP
                     NKTIGGLVGAFIGAFVVLTLLGTISIGLLIAVAFGGLLGDLLESMVKRQAQVKDAGTW
                     LPGFGGLLDRIDSLLLVLPLAYLLG"
     gene            5315..7249
                     /locus_tag="VFC01_27785"
     CDS             5315..7249
                     /locus_tag="VFC01_27785"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN83454.1"
                     /translation="MEGTPFGRYQLIELLGRGGMGEVWRAHDTTIDRVVALKMLLPHY
                     AQDPDFETRFRREARAAARLDDPHVVPIHDVGEIDGRLYVTMRLINGVDLQTLLDAGP
                     LEAGRAVHIIEQVASALNDAHQAGLVHRDVKPSNILLTHNDFAYLIDFGIARVVGETA
                     LTSANTTIGTWAYMAPERFSKGEIEPSSDIYALTCVLYQCLTGQPPYPGEALEQIAVG
                     HMVAPPPRPSEERGTVPMAMDRVIATGLAKQPTDRYPSTVDMAAAARRAITEPTTSPV
                     PPVPPDSAQTQPWHANRGAPPTMPARLGAPSIAPVPPNWAPAAKPPGRRRGVLIGALA
                     AVAVLVVGGVIVGVTLTQRDNRATTVPPTTATPGERGVGAPANTGAFTGTYRADFSAP
                     TLIEGGPLEGTTPQTATWAIRSVCRPGGCVATASRVSGDGPPVTSLVFDQIEGRWFAV
                     GTGSEQCQGAPAEIWVVLTLQPRPDGTLTGEATGTTSNTCSGRQTVTFTRTGDVDVDS
                     LPDPASLPPRVVSPAEGLRGRYHSTRKFTGGFGLMEGDYQVVTNCLRTGDRCMSYFHA
                     PADFRPLVFNGGTWTLDTSNEGKCDDGRIFDLRATGQYPMPQPSQDPIPQLTGHGHWE
                     QTGACAVTTDFDETFTRTGD"
     gene            7462..7719
                     /locus_tag="VFC01_27790"
     CDS             7462..7719
                     /locus_tag="VFC01_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020726482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83455.1"
                     /translation="MNTTHNLKKVIVGALLSGGVALAGLGLTTGTAQAAPQGPGPTID
                     HSDFGVPVVECIQCGRNLPGKLDIPSINPAGKVPVAVQGVG"
     gene            complement(7853..9541)
                     /locus_tag="VFC01_27795"
     CDS             complement(7853..9541)
                     /locus_tag="VFC01_27795"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN83456.1"
                     /translation="MEGTPFGRYQLLELLGRGGMGEVWKAFDTAMNRMVALKVLPGNL
                     ADDAQYQARFRREAQAAASLDEPHIVPIHDFGEIDGRLYVTMRLIDGKTIDELVANGP
                     LAPPRAVSIVEQIAAALSAAHRIGLVHRDVKPSNILVTEDDFAYLIDFGIARAAEATK
                     LTSTGATIGTLAYMAPERFTSDRTDARADIYALTCVLHECLTGCQPFPGDSLERQITN
                     HLTMPPPRPSTMQPGISPQMDQVIATGMAKNPDHRYATTKDLAVGARAALTAPIPHAP
                     PAPFTSPPPAPSWSAPVPYPDTGPSPAPASAATQYNAPPQYNAPPQPAPWSPPAMQSP
                     SPWWHNPAVWILAGLVTVVVIVIAVVAVIVWPDGSSTNRPTTRAQPTEQRLPSAPGTK
                     SSAPGTNTTVPGSTSAPTSTQSTPTGPTQLQTSDGLSGLLATMREKFGDTMGFQLVVY
                     PAYAVLERVSPNNSHVEQDFMYRDGKWNGWGADTTTSSFDVLADLSAFDANAVAATLA
                     GAPQALGAPDGSQTYLIVSGAEGGGLELAIHSAAPGTGYMQVNADGSVKKVFPP"
     gene            complement(9603..10187)
                     /locus_tag="VFC01_27800"
     CDS             complement(9603..10187)
                     /locus_tag="VFC01_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZN83457.1"
                     /translation="MRDLHRRTVLLSAGALFGVAGSLVSGAARAFGEPGLTDVPDLAL
                     LRRTFVLANEARESGGAPYGALVAGADGKVIAEHRNTAWADGGDRTQHAELVATTSAW
                     RELGIDGMNQATLYASTEPCSMCAGASYWNGIGRVAYGMSNLRLFEFTGDDPAHTSFA
                     LPCREILCNGSRPITVVGPLLEDEAAQSHVGYWH"
     gene            complement(10199..10648)
                     /locus_tag="VFC01_27805"
     CDS             complement(10199..10648)
                     /locus_tag="VFC01_27805"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZN83458.1"
                     /translation="MGAETDGITTSVQTWSGPVRAGLHPATGGDGSDACSGDMLLQAV
                     LACAGVTMRSVATAMGIDIVDAELHATGMFDARGTLGVSRDVPIGVQEISVVAELQTD
                     ADEATLAKLGELTERYCVVGQSLAHKLSIVVNKRAATGSPRHGVVDG"
     gene            complement(10661..11353)
                     /locus_tag="VFC01_27810"
     CDS             complement(10661..11353)
                     /locus_tag="VFC01_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014359618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZN83459.1"
                     /translation="MATRERRQSSRAVRAVVLHGEGKSFCSGLDIASFMSGEGGTGIL
                     LARDDDRLANFAQRVTYDWSLVPAPVIAAIHGNCFGGGLQIALGADIRIAAPDAKLCI
                     MEIKWGLVPDMGITQTLPRLVPIDVAKELTFSGRIVSGSEGLELGLVTRTADDPLSAA
                     LSLADEIAQKSPDAVRAAKRLYDETWASNDTAAALVLETELQTGLIGKPNQIAAVVAG
                     MSGEPPVFVDPA"
BASE COUNT         2050 a         3839 c         3644 g         1853 t
ORIGIN      
        1 caatcgcgct gggatggtgg ccgctggcgg ccctgttcct cgcccttcga ctggccggcg
       61 ccaacctcga cggcgctgtc gcccgcgccc gcggcgtgag ccggccgtgg ggattcgtgg
      121 tcaacgaaat cggcgaccgc acctcggacc tgctgacctt cgccggcctg gcggtcttcg
      181 cggcgcgaca gcacggcccc ggcttccaat ggctgtcctg gtctgtgctt caggtgctgg
      241 tggccgcact ggccgcgacg ctgccgacgt tcgcatccct ggccgcagcg gccgcaggcg
      301 cgacgcggcg caatggtggc ccgctgggca agaccgagcg ttgcttgttc gtcctgctgg
      361 ccacggcgtt tccggtcatc atgccgatcg tcctgacgca gctggtgaac ggttcgatcc
      421 tcaccaccgt gctgcggctg cgtgcggcgc gccgcgagct ggcggagaag cgccagcagg
      481 agcaggagcg tgccgaggtg gccgagcaga ctgccgacac ccgcccgatc ttgtcggtgg
      541 tggcagcatg aacgcgatct cctacgtgct acggcactgg atgtggcggc tggtgagcgc
      601 gctttcgggc gggctgaccg tgagcggcaa gtggctcgca tccggcggct gcatcgtggt
      661 ggccaaccac tcctcgcatg ccgacaccgc ggtactgctg gccgctcttc cccccaaggc
      721 caagcccgtg ttcggggcgg ctgccgacta ttggttcgac gtcccggtcc gccggttcat
      781 cgcgacctcg ctggccggtg tgctgccggt gcggcggtcc ggcggcggct cgtacgacgc
      841 gctgctggct gcggctcgcc ccgcgctgaa ggccggacgc accgtcgtca tctaccccga
      901 gggcactcgc tcgaccgatg gcaccatcgg cgagttccgt tccggcgcat tgcggttggc
      961 gcgggaatgc ggtgtgccga tcgtgccggt cgcgatgacg ggcactgccg acgtgctgcc
     1021 caagggcggc cgctggtctc ccgccccgat gcaggtccgc atcggcgaac ccgtcgatcc
     1081 gaccactgcc tcggctgcgg agctgcgcga gcacgtgctg gcattgcgcg acggcctgca
     1141 gccggacgtc gtcaggcgct atgcgctggc ggcgtgagcg ctgctacttc atcgtggacg
     1201 cgaggcgaat cttccaccgg acgacgctgg cgtagcacac cgagagcact ccaagcatga
     1261 cgatgtcgaa cgtccatgcc gacgcggtgt gcttccagtg ggagtcgtga gggatgaccg
     1321 ccaccgtatt cgtcagatcg accgtcgacg cggtggcggc caagccccat cgcgccgggg
     1381 taagccacga gaccgggtcc agcggccggt tcgtcaccgg gataaagccg ccggcgagca
     1441 ccagctgtgt catgaccgtc accaccatca gtggcagaac ctgattgctg ttctgcgcca
     1501 acgccgaaac gaagaggcca acgatcgccg ccgccacaca ggtcgccgcg atgtcgacaa
     1561 acagctccaa cctcggcgcg cccagcacca ccgcgccgag cggcgcgccc ttgccgatcg
     1621 tgggcgctgt cacgatgagc accaggatcg ccgactgaac caccgcggcc gtcccgaaga
     1681 ccgcgatctt ggccgcgaga tacgccgacg ccgacaaccc cgccgtctgt tcgcgacgga
     1741 agatgacgcg ttcgccgacc aaatcccgaa ttgtgagcgc cgtccccatg aagatcgcgc
     1801 cgaggttcag caagaccacg atctgcttgg gctcgttcgg tgaggcgctc tctaaggcgg
     1861 ctttgccgaa gccggtgtcg ccggccaccg caagtggcag cagtccgagg aggaacggca
     1921 ttagcaccaa gaacatgaaa tagccgcggt cggcccgaat aagccgcacc tggcggcgag
     1981 caatcgtcga gaattggcgc catagcccgg ttcgcacggg tttccccagg gccgcaaggt
     2041 gttccggcgc ggccgggccg gcagatccga cggggtgctc ggcgcgctca aggaatcgcc
     2101 tgtgggccgc atcgggatcg gcgccgatgt gggtgaagat gtcggcccag tcggtcgttc
     2161 ccatcgccgg tcccaattca ctcggcggtc cacagaatgc ggtcttaccc ccgggcgcta
     2221 gcaacagcac ctgatcgcag acgtccaggt acgccaatga gtgcgtcacc accacgacaa
     2281 tgcggccagc gtcagcaagc tggcgcagca tcttcatcac gacctgatcg agagcggggt
     2341 caagcccagt cgtgggctca tcgaggacca gcagcgacgg cccggtcaac agttccaacc
     2401 cgaccgacgc gcgcttacgc tgtccgcccg acaacttatc gatgcgtgtt tcggcgtgcg
     2461 gcgtcatttc cagttcctcg agcacctgcc gcaccaccca ctgccgctct tctttggtgg
     2521 tgtctggcga catccgcaat tccgccgcgt agcccaacgc ctggttgacg gtgagctgcc
     2581 gatgcacgac gtcgtcctgc ggcaccatcc caattcggtt gcgcaacgac gcgtactcgg
     2641 cgtgaatgtt gtgcccgtcg aacgacaccg ccccgccggt gggccgcgct ccgcccacca
     2701 tcacgttcaa caggctggac ttccccgaac ccgatggtcc gatcaccgcc gtcagcgtgg
     2761 ctggtttggc ggagaacgaa acccggtcca gcagtgtgtg attgccgccg atcgtcagac
     2821 tcacgtcgca cacatcgagg ccaccggact tcgcggcagg ttctgtgcga cgaacgagcg
     2881 tgccgttggc gaagaccatg tccacgttgg cgatcgtgac gacgtcgttc tcactcaagt
     2941 aggcgtcctt gacccgctgg ccgttgacaa aggtgccgtt gctgctgatg tcctggatct
     3001 gcatcccctg cgcagtcggt accaggcggg cgtggtggcg tgaggcaagc aggtcgccga
     3061 tgacgatgtc gttgtcgact tcgcgcccga tggtggtcga tccgggtggc gcggctgcgg
     3121 gtacgccggg ccgcaagaaa cggatcaacg tagtgacgat gtccaccacg tctgtcttga
     3181 cgtcggcatc gcgcttcccc ggaatcgtgg ttcgatcatc cggcgggaag tccgccgatt
     3241 cgccgtcatg gccgagtccg aacgtcaccc gcggaccgtt agggtcaccg atgtggatcg
     3301 cctgcccgtc tcgaatgttc acagacgaca ctcgctcgcg atcgacgaac atgccgttcg
     3361 tgcttgagtt gtcgatcgcg acccagtgat ggtcgaggta acacagcacc acgtgagccc
     3421 gcgagaccat tggctgcggc agccgcacgt ccgcatgcac atcgcgcccg atcacgacgt
     3481 cgccacccgg cccgaacgtg cggtgccaac caccggcgta cacggtgatc gtaggcggca
     3541 gcggccgact catctgaaca atctatcggt ccgcgttacg accacgcggc aatcaccgcg
     3601 gaaagtcccg cagtgaacag cacgcacacg atgatcatgt acaccccgta gagccgtcgc
     3661 agcaaggggt gcaacagctt tgacagcagc cagaccgcca gcccgaccgt cgagattcgc
     3721 agcccacgct ccagcagggt ccagccggcc agctgccaca ggtcgatatg gtgctcaccg
     3781 gctgcgcggg cgtacacctt gacggggatg cccgacaacg cctgattcat gatcgcgctc
     3841 ggccccgcgg agaactggtc gaatgccgtc gccgccatcc gcgtcgtagt cagtggcgct
     3901 ggcaacaaca caccgcgtga cgcgagccac gcgttggtca gcacgccgag caccgaaccc
     3961 ccgatcaccg ctgccgcaca tggcatcacg cggcgcggaa cggcggcggc gaacagaagc
     4021 agcgccatct cggcaacgat gaaccaactc agcgcctcgg cgaaacccca taggaacgcc
     4081 aacggcaggc cgtacgggcc gccgattacg cgggcgatcg ccgaccagac acgtgatcga
     4141 cgctcctgca tgtcgtcccg gtcatccacg atgacccccg aaatacacac gacaagaacg
     4201 caattcgccg gtcatccgag gaggtacgcc aacgggagca cgagcagcaa cgagtcaatc
     4261 cggtcgagca gacctccgaa tcccggcagc cacgtgccag cgtccttcac ttgagcctga
     4321 cgcttcacca tcgactcgag caagtcgccg agcaagccgc cgaacgcgac cgcgatcagc
     4381 agcccgatcg aaatcgtgcc gagcaacgtg agcaccacga acgccccgat gaacgcgccg
     4441 accagtccgc cgatcgtctt gtttgggctc aacggggaca acggcttacg cgcccagccg
     4501 aagcggcgca acccttttcc gccacaccag gcggcgacgt cggcggcgga tgcggcgaag
     4561 gccaccagga aggtgtctgg ccacaccatc accaggtggg ccagggacca gcagatccac
     4621 accgaaccga aggcggtgaa cgcggtccgc ctgccaccgt gctcgacgtc accgccgagc
     4681 accgagggca gcgcacaggc caacacgatg atcggcgcga ggcccaacag cgatgggcgc
     4741 agccatgccg ccaacgggta caggacaccc aacgccagca agattccggt atcgaccctg
     4801 ctgagtctca ccaggcgcgc gtactccatc accgcgacga ctgccagagc ggcggccaaa
     4861 gcggcggtgg ggccgcgacc catccagacc gggattccga ctatcggtgc gatgagcacc
     4921 cacgtggtcc acttctggat caattcccgc ttgcgtgacg caaagacggc gattccgccg
     4981 atcaccagga tcgccaccgt gacccacatg aagaagacgg ctcgggcgta gaggtggatg
     5041 tgcagtgggc cgaggtcgag gttgagatgc atccgccgat cgaaatgaac aatcgacagg
     5101 taccagtcga tgagcatcag cggggcccag tcggtactcg gccgacaggc ctcgcgtcca
     5161 ttaagcctcc tggcagactg cggttcaggg acacgataag ccgtaccgac agatccggta
     5221 cccgaaaacg gatgtcgggc attcgacatc ggaagttcgt cgtcgtattc gattcggccg
     5281 taaccgcggt ggctgacagg tttatcgtga gccggtggaa ggcacgcctt ttggccgcta
     5341 ccagctgatt gagctgctgg gccgcggcgg aatgggcgaa gtgtggcgcg cccacgacac
     5401 caccatcgac cgcgtggtgg cgttgaagat gctgctgccc cactacgcac aggaccctga
     5461 cttcgagacg cggttccgcc gcgaagcccg cgccgcagcg cgactcgacg acccgcatgt
     5521 cgtaccgatc catgacgtcg gcgagatcga cggccggctg tatgtgacca tgcggctgat
     5581 caacggcgtc gacctgcaaa ccctgttgga cgccggaccg ctcgaggccg gccgcgcggt
     5641 ccacatcatc gagcaggtcg cgtcggcgct caacgacgcg caccaggcgg gactggtgca
     5701 ccgcgatgtc aaaccgtcca acattctgtt gacgcacaac gactttgcgt acctcatcga
     5761 cttcggcatc gcccgcgttg ttggtgaaac cgcgttgacg tccgccaaca ccacgatcgg
     5821 cacttgggcc tacatggcac ccgagcgctt cagcaagggt gagatcgaac ccagctcgga
     5881 catctacgca ttgacatgcg tgctctacca atgcctgacc ggtcaacccc cgtacccggg
     5941 cgaggcgctg gagcagatcg cggtgggcca catggtcgcg ccgccgccca ggccgtccga
     6001 ggagcgcggc acagttccga tggcgatgga tcgcgtgatc gccaccggcc tggccaagca
     6061 accgaccgac cgctatccga gcaccgtgga catggccgca gcagcccgcc gggccatcac
     6121 cgagccgacg acgagtccgg tcccacccgt cccaccggac tcggcgcaga cccaaccgtg
     6181 gcacgccaat cgcggtgcgc caccgaccat gcccgcacgg ctcggcgcac catccattgc
     6241 gccggtgccg ccgaactggg caccggccgc gaagccgcca ggccggcgcc gcggcgtctt
     6301 gattggcgct cttgccgctg tcgcggtgct ggtcgtgggt ggcgtcatcg tcggtgtgac
     6361 gctcactcag cgcgacaacc gcgccaccac cgtcccgccc accaccgcca ccccaggtga
     6421 gaggggcgtc ggcgcgccag ccaacacggg tgcctttacc ggtacgtacc gggcggactt
     6481 cagcgcacca accctcatcg agggcggacc gctcgaaggt acgacgccgc agacggccac
     6541 atgggcaatc cgttcggtgt gccgcccagg cgggtgcgtg gcgacggcct cacgcgtgag
     6601 cggcgacggt ccgccggtga cgagcctggt gttcgaccag attgaagggc gctggttcgc
     6661 cgtgggcacc ggctcagaac aatgccaggg cgcacccgcc gaaatctggg ttgtgttgac
     6721 gttgcagcca cgccccgacg gcaccctcac gggcgaagct acgggaacaa ccagcaatac
     6781 gtgctcggga cgacaaaccg tgacgttcac ccgcacgggg gacgtcgacg tcgacagcct
     6841 ccccgatccg gccagcctcc cgccgcgggt cgtgtcgccc gccgaagggt tgcgcggtcg
     6901 ctatcacagc acgcgaaagt tcaccggcgg cttcggacta atggaaggcg actaccaagt
     6961 cgtcaccaac tgtcttcgca cgggcgaccg gtgcatgagc tatttccatg cgccagccga
     7021 tttccggccg ttggttttca acggcgggac ctggacgctg gacacctcga acgagggcaa
     7081 gtgtgacgac ggcaggatct tcgacctgag ggccaccggc cagtatccga tgccacaacc
     7141 atcacaggac ccgatcccgc agctcaccgg gcacggtcac tgggagcaga ccggggcgtg
     7201 cgcggtcacc acggacttcg acgaaacatt cacgcgcaca ggcgattagc actccgtcgc
     7261 ccgatgcccg cgcagacagc aacttcacgt atctatcgaa aaacctattg agccttggtc
     7321 attggccgcg cagcggcaaa ccagctaccg cctcaagaat tcgacaagaa gagtccaagg
     7381 atgcgtttag cggccggtcc tacgttcgcg tcgtcaactc gaccgcggtc cacccgggac
     7441 cgcctgacca aaggaaacaa catgaacacc actcacaacc tgaagaaggt gattgtcggg
     7501 gcgctgctgt ccggcggcgt cgcgctggcc ggattggggc tcaccacagg cacggcccag
     7561 gccgcgccgc aaggacctgg cccgacgatc gaccactctg acttcggtgt ccccgtagtc
     7621 gagtgcatcc agtgcggtcg caacctcccc ggcaagctgg acatccccag catcaacccg
     7681 gccggcaagg tgcccgtcgc cgtccaaggc gtcggctaag aacatcgttt ggccgtccgc
     7741 cggttcgccg gtgggcgggt cgaacgcgcg ctggtggact gctgcgaatc tacgaaacat
     7801 tcacgtgcac aggcgattag cgctgcgtcg cacaggcccg cccgccgggt cgtcacggcg
     7861 gaaaaacctt cttgacgctg ccgtccgcat tgacttgcat gtaacccgta cctggggccg
     7921 cggaatggat cgccagctcc aggccgccac cctcagcgcc cgagacgatc aggtaggtct
     7981 ggctgccgtc gggcgcaccg agggcttggg gcgcaccagc caacgtggcg gccaccgcgt
     8041 tcgcgtcgaa tgcgctgaga tcggcgagca cgtcgaacga actagtcgtc gtgtccgccc
     8101 cccaaccgtt ccacttgccg tcgcggtaca tgaagtcctg ttcgacgtgg ctgttgttcg
     8161 gggagacgcg ctcgagcacc gcgtaggcgg ggtagaccac caactggaat cccattgtgt
     8221 ccccgaactt ttctcgcatg gttgccagca ggccgctcag cccgtcggac gtctggagtt
     8281 gtgtcggtcc ggtgggcgtc gactgggttg aggtcggtgc agaggtactg cccgggaccg
     8341 tcgtgttggt gccgggcgcg gatgacttcg tgcctggcgc agacgggagc cgctgttcgg
     8401 ttggctgagc cctcgtggtc gggcgattgg ttgaactccc gtcaggccaa acgatgactg
     8461 cgacgacggc gatgacaatg acgacgacgg tcactaggcc cgccaaaatc cagacggcgg
     8521 ggttgtgcca ccagggagat ggagactgca tggcgggcgg agaccaaggc gcgggttggg
     8581 gaggcgcgtt gtattgggga ggcgcgttgt attgggtggc tgcgctcgcc ggcgctgggc
     8641 tcggccccgt atcggggtaa gggaccggcg cggaccaact gggtgcaggc ggtggcgagg
     8701 taaacggtgc gggcggagcg tgcgggatcg gcgcggttag cgcggcgcgg gcaccaaccg
     8761 ccaggtcttt ggtcgtggcg tagcggtggt cggggttctt ggccatgccg gtcgcgatga
     8821 cctggtccat ctgtggtgaa atcccgggct gcatcgtcga tggccgtggc ggcggcattg
     8881 tgaggtggtt ggtgatctgg cgttccaagc tgtcgcccgg aaatggctgg cacccggtca
     8941 gacactcgtg cagcacacac gtcaacgcat agatgtcggc gcgcgcatca gtacggtcgc
     9001 tggtgaatcg ctcaggcgcc atgtacgcca aggtgccgat cgtggcaccc gtgctggtga
     9061 gcttggtcgc ctcggccgca cgggcgatcc cgaaatcaat gaggtatgcg aagtcgtcct
     9121 cggtaaccaa gatgttcgat ggtttgacgt cgcggtgtac cagtccgatg cggtgcgctg
     9181 cgctgagggc ggcggcaatc tgttcaacga tcgagacggc gcgtgggggc gccagtgggc
     9241 cgttggcgac cagttcatct atggttttgc cgtcgatcag ccgcatcgtc acgtagaggc
     9301 gtccgtcgat ctcgccgaaa tcgtggatcg ggacgatatg aggctcgtcg aggctggccg
     9361 ctgcctgggc ctcccgacgg aaccgggctt gatactgcgc atcgtcggcg aggttcccgg
     9421 gtagcacctt caaggcgacc atccgattca tggcggtgtc gaacgccttc cacacttcgc
     9481 ccatgccgcc acggcccagc agctccaaca actggtatcg gccgaaaggt gtcccctcca
     9541 ccggctaacc ataagagcca gtcgcttcac ctgctagcgg attggctacg gccccgggag
     9601 gcttagtgcc aataccccac atgggattgc gctgcttcat cttcgagcag tgggccaacg
     9661 acggtgatcg ggcgcgagcc gttgcaaagg atctcgcgac aaggcagcgc aaacgaagtg
     9721 tgcgcgggat catcgccggt gaattcgaac agtcgaaggt tcgacatgcc gtaggcgacc
     9781 ctgccgatcc cgttccagta acttgcgccc gcgcacatcg aacacggctc agtactcgcg
     9841 tacaacgtcg cttggttcat cccgtcgata ccgagctcgc gccatgcact agttgtggcg
     9901 accaactctg cgtgctgcgt ccggtcgcca ccgtctgccc acgccgtatt gcgatgttcg
     9961 gcaatcacct tgccgtcggc accggcgacc agtgcaccgt aaggggcgcc tccgctctcg
    10021 cgggcctcgt tggccaacac aaatgttcgc cgaagcagtg caaggtcggg cacatcagtc
    10081 aggccgggct cgccgaaagc ccgggctgcg cccgaaacga gcgatccagc cactccgaat
    10141 agcgccccag cgctcagcaa caccgtccgc cggtgcagat ctcgcatggt cgacgatgtt
    10201 atccgtcgac aacaccatgt cgaggagatc ctgttgccgc acgtttattg acgacgattg
    10261 agagcttgtg cgccaagctc tgacccacaa cgcagtagcg ttccgtcagc tcgcccagct
    10321 tggccaaagt cgcttcatcg gcatcagttt gcagctcggc gacaacgctg atctcctgca
    10381 cgccgatcgg cacgtcgcgg gagacaccca aagtgccacg ggcgtcgaac atcccggtcg
    10441 catgcagctc ggcgtcgacg atatcgatgc ccatggcggt ggcgacgctg cgcatcgtca
    10501 cccccgcgca agcgagcacg gcctgcagca gcatgtcgcc ggagcaggcg tcgcttccgt
    10561 ccccgccagt ggccggatgc agacccgcac ggaccgggcc cgaccaagtc tgcaccgaag
    10621 tggtgatgcc gtcggtctcg gcgcccacct cgccgtcgcg ctacgcgggg tccacgaaga
    10681 ccggcggctc ccccgacatt ccagcgacga cagcagcgat ctgattgggc ttgccgataa
    10741 gcccggtctg cagctcggtt tcgagcacca gcgcggccgc ggtgtcgttg ctggcccagg
    10801 tctcgtcgta aagacgtttt gccgcgcgga ccgcgtccgg tgatttctgc gcgatctcgt
    10861 ccgcgagtga cagcgccgca gaaagcggat cgtcggcagt ccgagtgacg agcccgagct
    10921 ccaaaccctc actcccagaa acgatgcgac cgctaaacgt caactctttc gcgacgtcga
    10981 tcggtacgag ccgcggcagt gtctgcgtga tccccatgtc gggaacgagg ccccacttga
    11041 tctccatgat gcacagcttc gcgtccggcg cggcgattcg gatgtcggca ccgagcgcaa
    11101 tctgtagccc gccgccgaag cagttgccgt ggatcgccgc gatcactggt gccggcacca
    11161 acgaccagtc gtaggtcacg cgctgagcga agttcgccag ccggtcgtcg tctcgcgcca
    11221 acagaatgcc agtgccgcct tctccggaca tgaagcttgc tatgtccagg ccggagcaga
    11281 agctcttgcc ctcgccgtgc agcaccaccg cacgaaccgc acgcgaactc tgtcttcgct
    11341 cacgcgtggc gatctatcgc ggcgtcagaa cccaggtctc gatgag
//
LOCUS       DASRJA010000618         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1202204,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000618 DASRJA010000000
VERSION     DASRJA010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202204"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFC01_27815"
     CDS             complement(<1..194)
                     /locus_tag="VFC01_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE-family protein MCE1A"
                     /protein_id="HZN83460.1"
                     /translation="MPLDRHRPPYKTAGTVFLVIATVIAAFLYLQFRGDLTDKTQLTL
                     LSPRAGLVVERGSKVTFNGV"
     gene            211..606
                     /locus_tag="VFC01_27820"
     CDS             211..606
                     /locus_tag="VFC01_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier family protein"
                     /protein_id="HZN83461.1"
                     /translation="MAARRSADPAKTRAAVSAIVEWLRDEAQPEPSRTELGEAVRLTA
                     RTLAAIAPGASVEVRVPPFVAVQCISGPEHRRGNPPNVVETDPRTWLLLASGLLDVAG
                     AAATGALQLSGARAAEIAQWMPLVVVDGG"
     gene            733..2256
                     /gene="purF"
                     /locus_tag="VFC01_27825"
     CDS             733..2256
                     /gene="purF"
                     /locus_tag="VFC01_27825"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZN83462.1"
                     /translation="MTGPQTDQEPREECGVFGVWAPGEEVAKLTYYGLYALQHRGQEA
                     AGIAVADGSQVLVFKDLGLVSQVFDEQTLAAMEGHVAIGHCRYSTSGSTTWENAQPVF
                     RNTAAGTGVALGHNGNLVNGTELAGRAREAGLLGTRGAPAATTDSDILGALLAHGAAD
                     ATLEQAALELLPTVRGAFCLTFMDENTLYAARDPYGVRPLSLGRLDRGWVVASETAAL
                     DIVGASFVRDIEPGELLAIDADGVRSSRFAPPSPKGCVFEYVYLARPDSTIAGRSVHK
                     TRVDIGRRLAQEMPVEADLVIGVPESGIPAAVGYAQESGIPYGQGLTKNAYVGRTFIQ
                     PSQTIRQLGIRLKLNPLKEVIRGKRLIVVDDSIVRGNTQRALVRMLREAGAVEVHVRI
                     ASPPVKWPCFYGIDFATPAELIANAVENEDEMLEAVRHAIGADTLGYISQSGMIAATE
                     QPASRLCSACFDGNYPIELPGETALGKNVIEQMLATAARTGIPVQAENDNVSALRRP"
BASE COUNT          450 a          838 c          822 g          407 t
ORIGIN      
        1 tcaacgccgt tgaaggtcac cttcgacccc cgttcgacga caaggcccgc gcgcggcgac
       61 agcagtgtca gctgagtctt gtcggtgagg tcgccgcgga attgcagata gaggaaggcc
      121 gcgatgacgg tggcgatcac caggaagacc gtgcctgccg ttttataggg cggtcggtgg
      181 cggtctagcg gcactttgag aatctaaggt atggccgccc gccgcagtgc cgatccggcg
      241 aagacccgcg ccgccgtgtc ggccatcgtc gaatggttgc gcgacgaggc ccaacccgag
      301 ccgagtcgca ccgagttggg cgaggcggta cggctgacgg cacgcaccct cgcggcgatc
      361 gcaccggggg ccagcgtcga ggtacgcgtg ccgccgttcg ttgcggtgca atgcatttcg
      421 ggccccgaac atcggcgcgg caacccgccg aacgttgtgg agaccgatcc gcggacctgg
      481 ctgttgcttg ccagcggtct gctggatgtt gccggcgccg ccgcaaccgg cgcgctgcaa
      541 ctctccgggg cacgggcggc cgagatcgcg cagtggatgc cgctggttgt ggtcgacggc
      601 ggctagcgga aatgtctcgg gccccgaaat cgttgtggtg tgggcgacac cctgggtcgc
      661 gattccgtga ttaaccagtg accaacgtag actgaacggg tctacccacc acgccctggg
      721 agcagccata tcgtgaccgg cccgcagacc gatcaagaac cccgcgaaga gtgtggagta
      781 ttcggcgtct gggcgccggg cgaagaggtg gccaagctca cctactacgg cctttatgcg
      841 ttgcagcacc gcggccaaga agcggcgggc atcgccgtcg ccgacgggtc acaggtcctc
      901 gtattcaagg acctcgggct ggttagccag gtcttcgacg agcagaccct tgccgcgatg
      961 gaaggccacg tcgccatcgg ccactgccgc tactccacca gcggctccac cacatgggag
     1021 aacgcccaac cggtgttccg caacaccgcg gcaggcaccg gcgtcgcgct cggccacaac
     1081 ggcaacctgg tcaatggcac cgaactggca ggccgcgccc gcgaggccgg cctcttgggg
     1141 acgcgtggcg cgccggccgc cacgactgac tcggacatcc tcggcgcgct gctggcgcac
     1201 ggcgccgcgg acgccaccct cgagcaggcc gccctcgaac tgctgccgac ggtgcgcggc
     1261 gcgttctgcc tgacgttcat ggacgagaac accctgtacg cggcgcgcga cccgtacggc
     1321 gtgcggccgt tgtcgctggg acgcctcgac cgtggttggg tcgtcgcctc cgagacggcc
     1381 gcgctcgaca tcgtcggtgc ttcgttcgtg cgcgatatcg aaccaggcga actgctggcc
     1441 atcgacgccg acggcgttcg ctccagccgc ttcgccccgc cctcgccgaa gggctgtgtg
     1501 ttcgaatacg tgtacctggc ccggccggac agcacgattg ccggtcgatc ggtgcacaag
     1561 acgcgcgtag acatcgggcg caggctagcc caggagatgc ccgtcgaggc cgacctcgtg
     1621 atcggcgtac cggagtccgg tattccagcc gcggtcggct acgcgcagga gtccggcatc
     1681 ccctacggcc aaggccttac gaagaacgcc tacgtgggcc gcaccttcat ccagccgtcg
     1741 cagaccattc gccaactggg catccggctc aagctcaacc cgctcaaaga ggtgatccgc
     1801 ggcaagcggc tgatcgtcgt cgacgattcg atcgtgcgcg gcaacaccca gcgcgcactg
     1861 gtacggatgc tgcgcgaggc gggtgccgtc gaggtgcacg tgcggatcgc ctccccgccg
     1921 gtgaaatggc cgtgcttcta cggcatcgac ttcgccaccc ctgccgagct gatcgccaac
     1981 gccgtcgaga atgaggacga aatgctcgag gccgtgcggc acgcgatcgg ggccgacacc
     2041 ctcggctaca tctcacagtc gggcatgatc gcggccaccg agcagccggc atcccggctg
     2101 tgctccgcat gcttcgacgg caactacccg atcgagctgc ccggcgaaac ggcgttgggc
     2161 aagaacgtga tcgagcagat gctggccacc gccgcgcgca ctggtatccc cgttcaggcc
     2221 gagaacgaca acgtctctgc actgcgcagg ccctagcggc gaaaccggct ctagctgcga
     2281 aataacattt gttgtaattc gctggaatta ccttggtttg tgcccaagtg tgcccaaaat
     2341 gtgcccacgc gcgtgccgag agagctttca attcagctac gtcacaggaa gggggtttgc
     2401 taacgttcac ccccccaact aatttccgta agcaagtaag actccggcgc ctgcagcgca
     2461 acaggcccgg ggaatggccg aacttaatag gagattcgac gatgccaaag aatgtat
//
LOCUS       DASRJA010000619        21624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_925601,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000619 DASRJA010000000
VERSION     DASRJA010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 21624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21624
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925601"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..648
                     /locus_tag="VFC01_27830"
     CDS             178..648
                     /locus_tag="VFC01_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83463.1"
                     /translation="MPAVLTVGVPTATADGPTGGGGGGGATFVAFTQMFRRCDFSAKT
                     HIGPTAYGRPTATVRHTGSEVIADVQIATVLPNTPYDVGLVQMPRSSATGCNPGDPGV
                     ISGVLMTDPTGAGSVTVRGPIAPGASGVWIYISRPGEFSQTPDEFYTSDFVVSL"
     gene            complement(645..1253)
                     /locus_tag="VFC01_27835"
     CDS             complement(645..1253)
                     /locus_tag="VFC01_27835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83464.1"
                     /translation="MTGATLVTSSGYLTRPSRTRGRHRKQPPPRRFFSGRRLAVGGSA
                     VLLAALVVSVGSVVKWVTHEPVSQPNARPSTTVAPSPPTPSVEAPSPPAAIPADPFAV
                     DDRGFVNSSARCDGSQTAVAIGRTPGSLVVICGDQDGRYGYLGIRLSDDAVLKAAARS
                     TPTHEFIAHNANVTYSISPAELLVTAGGAVIKQEPMIDYRVP"
     gene            1412..1714
                     /locus_tag="VFC01_27840"
     CDS             1412..1714
                     /locus_tag="VFC01_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HZN83465.1"
                     /translation="MTVYAIAQLKFSDRNAYNRYQAAFMEVFQRYDGTLLAADEAPRV
                     IEGQWDLEKVVLISFPDEAAFREWSESPDYQRISEDRRAGADTVVVLVQGLPGNGT"
     gene            complement(1724..2470)
                     /gene="yaaA"
                     /locus_tag="VFC01_27845"
     CDS             complement(1724..2470)
                     /gene="yaaA"
                     /locus_tag="VFC01_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HZN83466.1"
                     /translation="MIVLLPPSETKHVGGDGPPLRLDALSSPDLGPLREALIDELIAL
                     ADDTSVCRGALGISASQDAEIERNAVLRSAATLPAISRYTGVLYDALDVGSLRGASAV
                     RARSRLAVGSALFGLLRADDPVPAYRLSATSKLPGRPTLAARWRPLLEPVLAQIASSE
                     LVVDLRSGSYAALGRVPGAVKVDVVSEDSDGHRTVVSHFNKAHKGRLARALATTRSEP
                     DDAAQVAAVGRRAGMRVERRGNGLTVVVPA"
     gene            complement(2501..3133)
                     /locus_tag="VFC01_27850"
     CDS             complement(2501..3133)
                     /locus_tag="VFC01_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HZN83467.1"
                     /translation="MFATVTLLTLVNSVSGTPYISGGDSPRGTDCSGLVSWVSNAASG
                     RPIYGDRFNTGNIERALLSRGFKYGSQPGALNVGWNGGHTAATLPDGTPVASGEGGGV
                     RVGGGGAFQGQFSHHMYLPMAPEDVPPAPDAPMAGDPFLPPPPEMIPPPAPEAPIVLM
                     AQEAPAPEMAPPPPPADPFAPPPPPADPFAPPPPADPFAPPAPGDIAPPA"
     gene            3727..5013
                     /locus_tag="VFC01_27855"
     CDS             3727..5013
                     /locus_tag="VFC01_27855"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83468.1"
                     /translation="MPDPQFDGMQAHFEMHRVRPVYDNKCPSVPNRAAVLIHGRTVTG
                     PVAFDLRYPAPGGGTLSVQEALARSGIDTFAPSLLGYGRSTTFDHGLDDPGNASLRPF
                     PPDGECPYPEGCDRSHNPIFPLDQQGALLAPNPLGGQRHTHSSNVRFANIDVWVRDIA
                     QAIDDAITRAQPTDGKATLIGYSLGALRVGRALYAAKYPAIVNTVDRVAFLSPFFGGP
                     PEETVPPGGFVTFPMTLMQRTQIVEGGRMANAEREAACAGYNVDGSGEQAWAQLMSLD
                     ANGRNWGGDDPRRPTGVLRLPTFSTYGFNDDVAGQLTQPTLVMQGLDDVVVPGGATSA
                     SAIYDALPGAMTNKVLVQLDCATHEMLWEGCSYAVRCDAASSTPYGTQPGKPWAGAYS
                     TVAAALIEWITNGTFDGTTVGRFTVDASGVARSTGT"
     gene            complement(5015..6151)
                     /locus_tag="VFC01_27860"
     CDS             complement(5015..6151)
                     /locus_tag="VFC01_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HZN83469.1"
                     /translation="MKVPQPDPDLPHLADVVPSVLSAMGVPGFDGPIPLPDGITGACV
                     LLVDGLGAELLDTYAADAPVMAGLRGRTLQVGFPSTTVAGLAAVGTGCRSGEHGMVGL
                     SFRLPGVDVVNALGWRPHPTGRDLRDKLPPEQVQPLPTTFERAAASGVAVSVITGAKF
                     AGSGLTRAVLRGGRYVGVHALGDLAACVRSALADGGFCYGYHSEIDLLGHVYGPGSVP
                     WRMQLRQVDRLVESIVEGLPPGALLAVVADHGMVALDASEVVDIDDCEPLMDGVKAIG
                     GEPRARHVYVADGAADSVLAAWRETLAQRAWVVSRDEAIAAGWFGDRVGDDARFRIGD
                     VVAAARGAAGIMRRTAEPLLSALIGQHGSLTDAEQRVPLLLAHG"
     gene            6334..6816
                     /locus_tag="VFC01_27865"
     CDS             6334..6816
                     /locus_tag="VFC01_27865"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZN83470.1"
                     /translation="MSTLPTVELGVLGPLQVRLDGAPVAIPGAKPRAVLTMLGLHSGS
                     VVSADTLVELLWGDDPPRTASKALQTHISSLRNTLGDGFVLTEGTGWTLHTTQIDASH
                     YKLAARMGRDAIAAGDTSEAMARFEAALASGRASRPATGPPRPRHWPMTSALTIADGS
                     "
     gene            6813..7496
                     /locus_tag="VFC01_27870"
     CDS             6813..7496
                     /locus_tag="VFC01_27870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83471.1"
                     /translation="MNIGTRHGRDAEIANARALAELRTTTITSIATRGDRLALSRHRV
                     FGRDQRPEAFHMEVLGILEINADSQLGARIVFDLDAIDAAVEELDARYLAGEAAAYSH
                     TWSAVIRAYASFNRRELPATTPDWLNIDHRRGAAFAPGDVIPYIRSAWDVAADISIYI
                     VAVHRLSHLGAVVTYAANGTSGEGFDAEWREITLLTFESDLISRCEIFDEVDLGAAIA
                     RFDELKSTL"
     gene            7572..8342
                     /locus_tag="VFC01_27875"
     CDS             7572..8342
                     /locus_tag="VFC01_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN83472.1"
                     /translation="MDEVFRALADPSRRLLLDSLNARNGQNLQELCAGLSMARQSVSK
                     HLAVLEAANLVTTVWRGREKLHYLNAEPINAIADRWINQYHRARVETLADLKTALETE
                     SMSSAEFVYTTFIRTTPERLWQAITDPAFSRRYMGHALVSDFTKGSTYVWEQDGLLIA
                     HPDQVILESDPYRRLAFTFHTFVPELTAAGLDEETIARAAAEKRSKVSFDIEPVDDDQ
                     VKLTVIHGDFGPDSTVRGLISGGWPWKIANLKTALEES"
     gene            8339..9058
                     /locus_tag="VFC01_27880"
     CDS             8339..9058
                     /locus_tag="VFC01_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HZN83473.1"
                     /translation="MTATPRLVDREAFQRELDELRVREKAHTRESDAIAAARRRLPMV
                     EVDGTTPLISASGPTTLLDAFEGRSQLIAYYFMWHTGHPAAEQCEGCTWCTTSVAELS
                     YLHSRDITYAVFCQGPYDESSRYRDFMGWDMPWYSAQDSLDTLLVGRQKNLMHLVCYL
                     RDGDRVFETYWTNYRGVEAMDYSFALMDLTTYGRQEEWEDSPGGWPIRPIANNPERVR
                     ENGRPIAQWSRLEAGRSDKLS"
     gene            9086..9829
                     /locus_tag="VFC01_27885"
     CDS             9086..9829
                     /locus_tag="VFC01_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HZN83474.1"
                     /translation="MTTPPVVSAQEWEAARQEMLVKEKEVQRARDALAAQRRRMPWLA
                     VEKEYVFDGPDGKVSLLDLFDGRRQLIIYRAFVEPGTGDWPEHGCVGCSLMADHVPNL
                     AHLNARNTTFVYASRASQPELERMKAKMGWSHIPWYTITDDFHKDFDVEDWHGTNAFI
                     RDGDRVFRTYFTNGRGDEVFVNTWNFLDMTALGRQEEWEDSPEGYPQTPPYEWWSWHD
                     NYAKHEPWRWFGDPDPNDPNDQRPPRKDA"
     gene            9908..10393
                     /locus_tag="VFC01_27890"
     CDS             9908..10393
                     /locus_tag="VFC01_27890"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN83475.1"
                     /translation="MNADVRRRRVTPRATDRAGDPSPFALGLALRRAHWHAAAVMSEA
                     LRPLGIELRHFAVLLVLVNRGPTEQRDLVAATGSDKAGIMRVVDDLERKGLAVRKPVP
                     GDRRVRAVEITPKGLELFDAAHEAAEPLAKRLVAPLKPAEAKQLTDLLTRFTSVASDE
                     K"
     gene            10489..11154
                     /locus_tag="VFC01_27895"
     CDS             10489..11154
                     /locus_tag="VFC01_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HZN83476.1"
                     /translation="MDVYEAVESRRAVRGFTDQPVSREVIERVLTAAAWSPSGSNIQP
                     WNIYVVTGAPLAELKKLAVERVATGVPWDEREYEMYPPAMKSPYSDRRAAFGKERYSA
                     LGIAREDWEARQRAAIANWDCFGAPAALFCYIDRDMGPAQWADLGMYLQTVMLLLRAE
                     GLDSCPQMAWSQVRETVADVVSPAEGQILFCGMSIGYEDKTVPYTRTGRAPLYETVTF
                     VDG"
     gene            complement(11156..11806)
                     /locus_tag="VFC01_27900"
     CDS             complement(11156..11806)
                     /locus_tag="VFC01_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN83477.1"
                     /translation="MSTTITQIRDDLWQTRTESPFPGLTTHAYLWRGPSGNVLFYSPA
                     SDADFDGIGDLGGVSAQYLSHLDEAGPTLARIADRFGSRLHAPAAELDAIGRHAHVDV
                     AIEGSRHLDANGVDAIPTPGHSDGSTCYLVRGVDGDTYLFTGDTMFPTEDGTWSTFLV
                     PGRGSAAELTSSLELLGTVQPNIVISSAFGGDTAIEKVDADRWQECVAEALASVPS"
     gene            11867..12235
                     /locus_tag="VFC01_27905"
     CDS             11867..12235
                     /locus_tag="VFC01_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83478.1"
                     /translation="MQAIAPMIACGLAAASIPLAGTASAVCDSRECVPNVARNVVQGG
                     PCAPRKTFVFGLDAGGATYVCTVAGAWSPVGPLIGVRNVPMSCPALNLSAQGSDGVPF
                     WCVDMGGGALQWAHRPDTVG"
     gene            complement(12237..12902)
                     /locus_tag="VFC01_27910"
     CDS             complement(12237..12902)
                     /locus_tag="VFC01_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN83479.1"
                     /translation="MQTVEYTHGRTLDLFDDKAGPTVLMWHGAQSDARSAMRPLAQLV
                     AGHGLTVVVPDWNSHANDHGRGDLLRSAEFARQHAGAGGLVVVGWSLGGVAAAALAIH
                     AAQFEVPVTHTVCLAGAFMAEDPITAAHLPTELPAQRSPFTLLHGAADDVVPVEASRS
                     FAATLSDNDWPVEFVELPADHGSIAGTTYDRALDRYAAADDPQVLAVATDVAGRIASV
                     VER"
     gene            complement(13256..13885)
                     /gene="upp"
                     /locus_tag="VFC01_27915"
     CDS             complement(13256..13885)
                     /gene="upp"
                     /locus_tag="VFC01_27915"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HZN83480.1"
                     /translation="MATVHLIEHPLVQHKLTLMRRKDASTNSFRQLLHEISALMAYEV
                     LRDVPLHDVEIDTPLESTTGRVIDGKKLVFVSILRAGSGILDGMLTVVPGARVGHIGL
                     YRDPKTLVAVEYYFKMPGELHERDVVVVDPMLATGNSAVAAVDRLKECGPKSVKFVCL
                     LTCPEGIAAMQNAHPDVPIYTAAIDRQLDEHGYILPGLGDAGDRIFGTK"
     gene            complement(13891..15144)
                     /locus_tag="VFC01_27920"
     CDS             complement(13891..15144)
                     /locus_tag="VFC01_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="URC4/urg3 family protein"
                     /protein_id="HZN83481.1"
                     /translation="MNLITMHPDLNADTPEGAAAALRTTTVVRAHARQLLHRARDGAS
                     RWFSVHDDALQAAAVEVADMMRTRYPDLSIPYHSRWRHFDAGGVDRKAELDDRLAELD
                     TPARARAMIDLTTVSVLLDAGAGPDWRYLETDSGQRFSRSEGLAVASWHAFLGGLFSS
                     DAAHPLRVDAAGLRALDAGKLAEAFQISPSNPLVGFEGRMALLHRLGEALTAQRELFG
                     THGRPGELFDLLVEHPGPVTAHDILSHLLLSLSGIWPTHNAIGDHPLGDCWRHQAVTG
                     SGLTRGWVPFHKLSQWLTYSLLEPFQWAGVYVQGQDALTGLPEYRNGGLLLDADVLRL
                     HDPSWSERTWTVGSELVVEWRALTVALLDELAPLVRARFGTDAEQMPLACVLEGGTWA
                     AGRALAERFRGGRPPISVVSDGTVF"
     gene            complement(15141..16427)
                     /locus_tag="VFC01_27925"
     CDS             complement(15141..16427)
                     /locus_tag="VFC01_27925"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012687643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HZN83482.1"
                     /translation="MSAEPGAAATSRAKSNSASGHIRLTSHSGGLDAPPIRWGAPTAA
                     ERGPLVGTTTKRAHRNVIGTHSGSYSVYRALAVAAGALSREHKADLTDTSPTDVIGPF
                     PQWSEPGKIVSLDPWGAMVADVFATELAAGYDIRPTIAITKAHVIVPEIMDAVAKGRL
                     VPDGRILLPNGAALVTKAAIEPVWHLPGVAHRFGCSENDLRRVLFEETGGMYPELVTR
                     SDLEVFLPPIGGQTIYIFGNGGDLGDPSVELTARVHDECNGSDVFGSDICTCRPYLTH
                     AIEECILGAQRGGVGLVAYSRKEGRALGEVTKFLVYNARKRQVGGDTAEAYFARTECV
                     AGVQDMRFQELMPDVLHWLGIRKIHRLVSMSNMKYDAITASGIEVGERVNIPDALIPP
                     DARVEIDAKMAAGYFTPGPVPDADELSKPKGREFEK"
     gene            17289..18551
                     /locus_tag="VFC01_27930"
     CDS             17289..18551
                     /locus_tag="VFC01_27930"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZN83483.1"
                     /translation="MRTGEIKALSGLRIVAAVWVVLFHFRPWLEVSAPGFRSALAPVL
                     NCGAQGVDLFFMLSGFVLTWNYLDRMGESFSIRSTLHFLWLRLARVWPVYLVTLHLAL
                     AWIIFTLYVGHLPSPVADQLNAVSYVRQVLLVQLWYQPFFDGSSWDGPAWSISAEWLA
                     YLLFGVLVLAVFRISRATRARGLLWLAIVASLPPALLLLATGHFYTPWSWLPRIVMQF
                     TAGALACAAVRKLQPTDRARRIAGYLSLALGIGIVGILYFLDAHTVPKVSDSSGLVDV
                     LFVPLVVTLAIGVGSLPRLLSTRIMVYGGYISFSLYMVHELVHTAWNWFTAQFAIVLE
                     PSWSAKLIVVGVLGVAVLGAAALYHFVEEPARIWMRNMVKVRDTQPVARDAPAEARTE
                     AADPAHSKLQPLHGPRHVPAKAVAARAG"
     gene            18669..19361
                     /locus_tag="VFC01_27935"
     CDS             18669..19361
                     /locus_tag="VFC01_27935"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL lipase"
                     /protein_id="HZN83484.1"
                     /translation="MSRLVTLFVAIAVLVGLLDARSTPVRHNDIITLESSLNHIAVVG
                     DSYTTGGELGGLGTKGWTTGAWQLLARQGIAVTADVAAEGGAGYGTRGNHGSVFEDLT
                     ADAVKPDDALVVFFGSRNDQGVDPTQLSILAWGTFQLARRTAPSAKFLVIGPPWPTAD
                     PPDAVLKIRDTLKYQADVVGATFIDPINAGWFVGRPELIGKDGVHPTDAGHAYMAEKI
                     APLIGAQLTRKT"
     gene            complement(19416..21575)
                     /gene="katG"
                     /locus_tag="VFC01_27940"
     CDS             complement(19416..21575)
                     /gene="katG"
                     /locus_tag="VFC01_27940"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007529876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HZN83485.1"
                     /translation="MSESENPAIKAPTSKQPGRPMSNQDWWPNQLDLQVLHQHSPRGN
                     PMDEDFDYAKEFATVDLGALKQDVIDVMTTSQEWWPADYGHYGPLFIRMSWHAAGTYR
                     IADGRGGGGEGAQRFAPLNSWPDNVSLDKARRLLWPVKKKYGRKISWADLLVFAGDCA
                     IESMGLKTFGFGFGREDIWEPEEIFWGPEDTWLGDERYAGDRELQGPFGAVQMGLIYV
                     NPEGPNGNPDPLAAARDIRETFRRMAMNDEETAALIVGGHSVGKTHGAGNADLVGPEP
                     EGCPLQFQGLGWVNPQGTGVGKDAITSGLEGIWTATPTTWDNSFLETLYAHEWELFQS
                     PAGANQWRPKDGAGADTVPEAFGNGKTHPTMLTTDLSLRMDPAYEKITRRWLDHPEEM
                     TDAFARAWYKLLHRDMGPISRYLGPWVAEPQLWQDPVPAVDHELITDEDAKALKAKIL
                     DSGLSVSQLVSAAWASASSFRGTDLRGGANGARIRLAPQKDWEANKTADLDTVLPALE
                     KIQSEFGKKVSLADLIVLGGSAAVEKAAKDAGVEITVPFAPGRTDASQEQTDVEAFAV
                     LEPRADGFRNYIRAGEKAPVERLLVEKAYLLNLTAPELTVLIGGLRVLGNGKSKHGVF
                     TDKPGVLTNDFFVNLLDMGTVWDVSQSTENVYEGRDRQTGAAKWTATANDLVFGSNSI
                     LRSLAEVYATDDAKEKFVADFVKAWVKVMNNDRFDLA"
BASE COUNT         3861 a         7114 c         6974 g         3675 t
ORIGIN      
        1 acgaagccca gctcgggcaa caccgtcatg ctttcgacgg tacgcgagtg gattggctgt
       61 ttcgccgtcc gacgaggcgt taattactaa tctcgatggc acgcggacac gaatgaacgg
      121 caggtagcaa cgatgcgtca gcgcgttctg aacactgtgg cagcaatggt tgtggcgatg
      181 ccggcggttc tgactgtcgg cgttccaacg gcgacggccg acggcccaac gggtggcggt
      241 ggtggtggtg gggcaacttt cgtggcattc actcagatgt tccgacggtg cgatttcagc
      301 gccaagacac atatcggacc gacggcgtac ggacgcccaa ccgcgacggt gcgccatacg
      361 ggctccgagg tgatcgccga cgtgcagatc gcgacggtcc tgccgaatac gccctacgac
      421 gtggggttgg tacagatgcc gcgctcatcg gcgacgggct gcaatcccgg cgatccgggc
      481 gtgatctccg gtgtgctcat gacagacccc acaggcgcag gcagcgtcac cgttcgcggc
      541 cccattgcgc ccggcgcgag cggcgtgtgg atttacatca gtcggccggg cgagttctct
      601 cagacaccgg acgagttcta cacctccgac ttcgtcgtat cgctctaggg aacccggtag
      661 tcgatcatcg gctcctgctt gatcaccgcg cctccggcgg tgaccagaag ctcagcgggt
      721 gagattgagt acgtgacgtt tgcgttgtgc gcgatgaact cgtgtgtcgg cgtgctcctg
      781 gccgccgcct tgaggaccgc atcgtcactg agccggatgc ccagatagcc gtagcgcccg
      841 tcctggtctc cgcatatcac caccagtgac cctggtgtcc ggccgattgc gacggccgtc
      901 tggcttccgt cgcatcgtgc actggagttg acgaagcccc tgtcatcgac cgcgaacggg
      961 tcagccggta tggccgccgg tgggcttggc gcttcgaccg acggcgttgg tgggctcggg
     1021 gcgaccgtcg tagacggacg agcgttcggt tgcgaaaccg gctcgtgtgt cacccacttc
     1081 accaccgagc caaccgagac gaccagcgcc gcaaggagga cggccgatcc gccgacggca
     1141 aggcgccggc ctgagaagaa ccggcgtggt ggtggctgct ttcgatgtcg gccgcgggtg
     1201 cgggaaggtc gcgtcagata ccccgagctg gtgaccaagg tcgctcccgt catgcaactt
     1261 cgtgcactag ttgtgcggaa cattctgcac gacacgatcc gcacgacaaa cgatggctac
     1321 gccgcgtcga acaggctcga atcttgttga ttcactggac aatttgctag ctacgcattg
     1381 acgcttgcga aagtgcggct acggtggccg ggtgaccgtt tacgccatcg ctcagctgaa
     1441 gttctccgac cgcaacgcct acaaccgcta tcaggcggcg ttcatggagg tgttccaacg
     1501 ctacgacggc acgctgttgg cggccgatga ggcgccgcgg gtcatcgagg gtcaatggga
     1561 cctagagaag gtcgtgttga tcagctttcc ggatgaggcg gcgttccgtg agtggtcgga
     1621 atcaccggac tatcaacgga tttcagagga ccgccgcgcg ggagccgaca cagtggtggt
     1681 gcttgtgcaa gggttgccgg gtaacgggac gtagcggatc acgttatgcg ggaacaacaa
     1741 ccgtcaatcc attgccgcga cgctccaccc gcataccggc ccgccgaccg actgcggcca
     1801 cctgcgcggc atcgtcgggc tcggaccttg tcgtggctag ggcgcgcgcc agacgaccct
     1861 tgtgcgcctt gttgaaatgg ctcaccacgg tgcggtggcc gtcagaatcc tcggacacca
     1921 catcgacctt gacggcgccg ggcacccttc ctaacgccgc ataggaaccg gatcgcaggt
     1981 cgaccactag ctcgctcgac gcaatctgtg ccagtaccgg ttcaagcaga ggacgccatc
     2041 gcgcagccag cgtgggccga ccgggcagct tcgatgtcgc ggagagccga tacgcgggca
     2101 ccggatcgtc cgcgcgcaac aggccaaaca gcgcggagcc gaccgccagc cggcttcggg
     2161 cgcgaacggc ggatgcaccg cgcaacgatc cgacatcaag ggcgtcgtac agcacgccgg
     2221 tgtagcgact aatggcgggc agcgtggctg cgctccgcag caccgcgttg cgctcgatct
     2281 cggcgtcctg cgacgcggag atacccagcg cgccccggca taccgaggtg tcgtcggcca
     2341 gcgcgatgag ttcgtcgatc agcgcttcgc gtaacggccc gaggtccgga gagctcaggg
     2401 catccagacg cagaggcggc ccgtcaccgc cgacgtgctt ggtctccgat ggcggcagca
     2461 gcacgatcac ggtcgtgacg ctaaccgacc ccgtgccggg tcaggccggg ggagcgatgt
     2521 cgccaggcgc cggcggcgcg aacggatccg ctggtggcgg cggcgcgaac ggatctgccg
     2581 gcgggggcgg cggcgcgaag ggatcggccg gcgggggcgg cggtgccatc tccggtgccg
     2641 gggcctcctg ggccatcagc acgataggcg cctccggtgc cggcggcggg atcatttcgg
     2701 gcgggggcgg aaggaacggg tcacccgcca tcggcgcgtc aggcgcgggc ggcacgtcct
     2761 caggtgccat cggcaggtac atgtggtgag agaactgtcc ctggaaggcg ccaccgccac
     2821 cgaccctcac gccgccgcct tcaccggagg ctacgggagt gccatcgggg agtgtcgccg
     2881 ccgtgtgacc gccgttccag ccgacgttca gggcaccggg ctgcgacccg tacttgaaac
     2941 cgcgggagag cagagccctc tcgatgttgc cggtgttgaa ccggtcgccg tagatcggac
     3001 ggccacttgc cgcgttagag acccatgaca ccaggcccga gcagtccgtg ccccggggag
     3061 agtctccacc ggagatatac ggggtgcccg aaaccgagtt cacgagcgtc aacagggtta
     3121 cggtagcaaa catgcggccg gacgctacta gaggtttttg taacgcgcca aaatctgtga
     3181 cgcccttcac aactcgtgct cagcatcggt tcacttcacg aaatgtcttg ggctgccaaa
     3241 agactcaata tgacgccgcc acttacgatt tcatgaattt tcgctggtag cagcgggtag
     3301 tttcatgcgc aatcgcgcgt acgcgcaggc catatagata tggaagcgct gcgacttcgt
     3361 ggcccgtggc gcacacatac cgcacgctga agccgcgcca caagtggcag gcatcacatt
     3421 tcaagatcag cgaatgtgac ccatgccaca gcgagcacgc acgtcaaacg cccaggtcga
     3481 acaccagcga tcgccggacc gctcgtcgtg ctgacgatta ggctgcaatc gacccagtga
     3541 ggtgcggaaa tggctgtctc gatcaggctc ggacgcttca gaactgcatt ggcgctgtgt
     3601 gtatgtctcc tcatggctag cagctcgctg atctcgacca cttcctgggc ggatgactgt
     3661 gacatcactc cgatagatgt cgaaacgacg gactattcgt tgccattcga ggtgccgggg
     3721 ggactgatgc ccgatcctca gttcgacggc atgcaggcac actttgaaat gcaccgcgtg
     3781 cgaccggttt acgacaacaa gtgcccatcg gtaccgaaca gggcggcagt gctgatccac
     3841 ggccgcacgg tcactggccc tgtggcgttc gatctgcgat accccgcgcc cggtggcggc
     3901 accctaagcg tgcaggaagc gttggcgcga agcggaattg acacattcgc accgagcctg
     3961 ctcggctacg gccgctcgac gacgttcgac catgggctgg acgatcccgg aaatgcaagc
     4021 ctgagaccat ttccgccaga tggcgagtgc ccatacccgg agggatgcga ccgatcccac
     4081 aatccgatct ttccgctcga tcaacaaggc gcgttgctcg cccccaatcc tcttggcgga
     4141 cagcgtcaca cgcattccag caatgtgcgt ttcgcgaata tcgacgtgtg ggttcgagac
     4201 attgctcagg cgatcgacga cgcgatcacc cgggcacagc ccactgacgg caaggcgaca
     4261 ttgatcggct actcgctcgg cgcgctgcgc gtcggccgtg ccctgtacgc ggccaaatac
     4321 cccgcaattg tcaacacagt cgatcgagtt gcgttcttgt caccgttctt tggcggacca
     4381 ccggaagaga ctgtgccgcc agggggcttt gtcacgtttc cgatgacgct catgcagcgc
     4441 acgcagatcg tcgaaggcgg caggatggcc aacgccgaaa gggaggccgc atgtgccggg
     4501 tacaacgtcg acggcagcgg cgagcaggcg tgggctcagc tgatgagcct cgacgccaat
     4561 ggtcgcaatt ggggcggtga cgaccctcgc cggcctaccg gggtgctgcg gttgccgacg
     4621 ttctccacat acggcttcaa tgatgacgtt gccggacagt tgacccagcc gacattggtc
     4681 atgcagggtc tcgacgacgt cgttgtgccc ggcggtgcca ccagcgcatc ggcgatctac
     4741 gatgcactgc ccggcgcgat gaccaataag gttctggtgc agctggattg cgcaacacac
     4801 gagatgttgt gggagggctg ttcgtacgcg gtgcgctgcg acgcggcttc cagcacccca
     4861 tatggaactc agccgggaaa gccttgggcc ggagcgtatt ccacagtcgc ggcggcattg
     4921 atcgaatgga tcaccaacgg gacattcgac ggcacaacgg tcggcaggtt cactgtcgat
     4981 gcgagcggcg tcgccaggtc gaccggaacc tgagtcagcc gtgggcgagc agcagcggca
     5041 cccgctgctc ggcgtcggtc agcgaaccat gttgtccgat caacgcagac aacagcggtt
     5101 ccgctgtgcg ccgcatgatc ccggcggcgc cgcgtgccgc cgcaacgacg tcgccgatgc
     5161 ggaaccgcgc atcgtcgccg acccggtcgc cgaaccatcc cgccgcgatc gcttcgtctc
     5221 gcgagaccac ccacgcccgc tgcgccaacg tctcccgcca cgccgcgagc accgaatctg
     5281 ccgcgccgtc ggcgacgtag acgtggcggg cgcgcggctc gccaccgatg gccttgacgc
     5341 cgtccatcaa gggctcgcaa tcgtcgatgt ccacaacctc tgacgcgtcg agcgcaacca
     5401 tgccgtggtc ggccacgacg gcgagcagcg cgcccggcgg caggccctcg acgatggact
     5461 ccaccagccg gtcgacctga cgcagttgca tccgccacgg caccgaaccc gggccgtaaa
     5521 catgccccag caggtcgatc tcggagtggt agccatagca gaagccgccg tcggcaagcg
     5581 cggaccgcac gcacgcggcc aggtcgccga gcgcatgcac gcccacgtac cgaccgccac
     5641 gcaggacggc gcgggtcagg ccggacccgg cgaatttcgc gccggtgatg acgctgaccg
     5701 cgactccgga cgccgcggcc cgctcgaacg tcgtcggcag gggctggacc tgctccggtg
     5761 gaagcttgtc acgcaggtcg cgccccgtcg ggtgcggacg ccatccgagg gcgttgacga
     5821 cgtcgacacc cgggagccga aacgacaggc ccaccatgcc gtgctcgccg gagcggcagc
     5881 cggtgccaac cgccgccagt ccggcgacgg tcgtggaagg aaacccgacc tgcagcgtgc
     5941 gcccccgcag cccggccatc actggagcgt cggcggcata ggtgtcgagc agctcggcgc
     6001 caagcccgtc cacgagcagc acacaggcgc cggtgatccc gtcgggcagg ggaatcggcc
     6061 cgtcgaaccc ggggacgccc atcgcggaca gcaccgacgg caccacgtcg gccaaatgcg
     6121 ggagatctgg atcgggctgc ggcaccttca cgattcgagc ctgtcacacc gccgagcggc
     6181 accgaggctc ttgtagcgaa ccatcggcga aggttcacca ggatgccctc gagaatcgac
     6241 gacatccgca tttctcctgc agcgcaacga gcgcgcgagg cagctttacc ggatcttgac
     6301 cgcgtgttga atcgccttac taaccatggc gaggtgagta cgcttccaac ggtggaactg
     6361 ggggttttgg gtccacttca agtccggctt gacggcgcgc ccgtcgcgat cccgggcgcg
     6421 aaaccgcgcg ccgtcctcac gatgctcggg ctgcactctg gctcggttgt gtcagccgac
     6481 acgctagtcg agctgctgtg gggcgatgat ccgccgcgga cggccagcaa agccctgcag
     6541 acgcacatct cttcgttgcg taacaccctc ggagatggct tcgtgttgac ggagggcacg
     6601 ggctggactc tgcacacgac ccagattgac gcctcgcact acaaattggc cgccaggatg
     6661 ggccgtgacg ccatcgctgc cggagacacc agcgaagcga tggcccgctt tgaagcggcg
     6721 ttggcttccg ggcgtgcttc gcggcccgcg actgggccgc cacggccgag acactggccg
     6781 atgacttctg cactgacgat cgccgacggg tcgtgaacat tgggacccgc cacggtcgag
     6841 atgccgaaat cgcgaatgcg cgtgcgttgg ccgaactccg gacgacgacc ataacctcaa
     6901 ttgcgacccg cggggaccgc ctcgccctca gccgtcaccg cgttttcggc cgcgaccagc
     6961 ggcccgaggc gtttcacatg gaagtgctcg gcatcctcga gatcaacgcc gacagtcaac
     7021 tcggggctcg catcgtcttc gatctcgacg ccatcgatgc cgccgtcgag gagcttgatg
     7081 cccgctacct cgccggcgaa gccgccgcct actcgcacac atggtccgct gtcatacggg
     7141 cgtatgcctc cttcaatcga cgcgagctac cggcgacgac gccggactgg ctgaacatcg
     7201 accaccggcg cggggcagca ttcgcgcccg gcgacgtgat cccgtacatc cgctccgcgt
     7261 gggacgtggc tgcagacatc agcatctaca tcgtggccgt acatcggctc agccacctcg
     7321 gtgctgtggt cacctatgca gcgaatggga cctcaggaga gggtttcgac gccgagtggc
     7381 gggagatcac gcttctgacg ttcgaaagcg acctgatcag ccgctgcgaa atcttcgacg
     7441 aggtggatct cggcgccgcg atcgcgaggt tcgacgaact caaatccacc ctctgaccat
     7501 tgacacgcag ccatccagct gcctatagtc agacatgcag ccagttagtt gcatataggg
     7561 aaatgacaga gatggacgag gtgttcaggg cgttggcaga tccgagccga cgtctgctgc
     7621 tcgacagcct caatgcgcgc aacggacaga acctgcagga gctgtgcgcg ggcttgtcga
     7681 tggcgcggca gtcggtcagc aagcatctcg cagtgcttga agcggccaac ctggtcacca
     7741 cggtatggcg gggtagggaa aagctgcact acctcaacgc cgaaccgatc aacgccatcg
     7801 ccgaccgctg gatcaaccaa taccaccgtg cgcgcgtaga gacgctcgcg gacttaaaaa
     7861 ccgcattgga gaccgaatca atgagcagcg ccgaattcgt ctatacgacg ttcatccgga
     7921 cgacgccgga gcgactctgg caagcgatca ccgatccagc gttctcgcgc cgctacatgg
     7981 gtcacgcgct ggtgtccgat ttcaccaagg ggtccacgta cgtctgggaa caggatggcc
     8041 tgctgatcgc acatcccgac caggtgatcc ttgaatccga tccgtaccgc cggctggcgt
     8101 tcaccttcca cactttcgtg cccgaactca cggccgccgg actggacgaa gaaacgatcg
     8161 ccagggcggc ggccgagaag cgctcgaagg tgtccttcga catcgagccg gttgacgacg
     8221 accaggtcaa gctcaccgtc atccatggcg acttcggacc ggacagcacg gtgcgcggac
     8281 tgatctccgg tgggtggccg tggaagatcg cgaacctcaa gactgcgctg gaggagtcat
     8341 gaccgccacg cctcgactgg tcgaccgcga agcgttccag cgtgaactcg acgagttgcg
     8401 ggttcgcgag aaggcccaca cgcgcgaatc agatgcgatc gccgcagcgc gccggcggtt
     8461 gccaatggtt gaggtggacg gcaccacgcc cctcatcagc gccagcggac cgaccacgtt
     8521 gctcgacgca ttcgagggcc gttctcaact gatcgcttat tacttcatgt ggcacaccgg
     8581 tcatccggca gccgaacaat gcgaaggctg cacgtggtgc accacaagcg tcgcagagtt
     8641 gtcttacctg cattcccgcg acatcaccta cgcggtcttc tgccagggcc cctacgacga
     8701 aagcagccgc taccgcgact tcatgggttg ggatatgccg tggtactcgg cccaggactc
     8761 actcgacacg ctgctggtcg ggcgccaaaa gaacttgatg cacctcgtgt gctacctccg
     8821 ggacggcgac cgtgtgtttg aaacctattg gacgaattac cgcggcgtag aggcgatgga
     8881 ctacagcttc gcgctcatgg acctcaccac gtacggacgc caggaggagt gggaagactc
     8941 gcccggcggc tggccgatac gtccgatcgc caacaacccc gaacgagtcc gcgagaacgg
     9001 acgtccgatc gcccagtggt cgcgacttga agccggacgc tcagacaagc tgagttaagt
     9061 attgaaagcc gaagggagac aaggaatgac aacacccccg gtcgtttcgg cgcaggagtg
     9121 ggaagctgcc cgacaagaaa tgctcgtcaa agagaaggaa gtacagcggg cccgggacgc
     9181 actggccgcg cagcgccgca ggatgccatg gctggcggtg gagaaggaat acgtgttcga
     9241 cggtcccgac ggcaaggtga gcctgcttga cctgttcgat ggacgccgcc aactgatcat
     9301 ttatcgcgca ttcgtcgaac cgggcacagg cgactggccc gaacacggtt gcgtcggttg
     9361 ctctttgatg gctgaccacg ttccgaacct cgcccacctg aacgcgcgca acaccacatt
     9421 cgtctacgcc tcacgcgcat cgcagcccga gctcgagcgg atgaaggcga agatgggctg
     9481 gagccacatc ccgtggtaca ccatcaccga cgacttccac aaagacttcg acgtcgagga
     9541 ttggcacggc accaacgcct tcatccgcga cggcgaccgc gtgttccgca cctacttcac
     9601 caacggccgc ggggatgaag tgtttgtcaa tacctggaac ttcctcgaca tgaccgcgct
     9661 tggacgccaa gaggaatggg aagattcgcc ggaaggctat ccgcagaccc cgccgtacga
     9721 gtggtggagc tggcacgaca attacgccaa gcacgaaccg tggcgttggt tcggcgaccc
     9781 ggacccgaac gaccctaacg accaacggcc gccgcgcaag gacgcatagg acggcggcgg
     9841 cccgggccgg catttgagct caatcgatac cgtatcgagt gatccgaagc ttcggtatgg
     9901 tcgattcatg aacgctgacg ttcgccgccg tcgggtaacc ccgcgtgcga ccgaccgcgc
     9961 cggggatcct tctccgttcg cgctcgggtt ggcgctgcgc cgggctcact ggcacgcggc
    10021 tgcggtgatg tcggaggcgc ttcggccgct cggcatcgag ctacggcatt ttgccgtgct
    10081 gctcgttctg gtcaaccgcg ggcccacgga gcaacgtgac ctagtagcgg caacgggctc
    10141 ggacaaggca ggaatcatgc gggtcgtcga cgacctcgag cgcaaggggc ttgccgttcg
    10201 caagcccgtc ccgggggacc ggcgcgtgcg ggctgtcgag atcacgccga agggacttga
    10261 gctgttcgac gccgcccacg aggccgccga accattggcc aagcgcctgg tcgcgccgct
    10321 gaagcccgcc gaagccaagc agttgacaga cctgctcacc cgattcacct ctgtggcttc
    10381 cgacgaaaag taacccctcc cgctcatcag gccgccagcg gggaatagta tcgatcaata
    10441 cggtattgaa tattactgta aggatcgaaa ctaagaaggc aggcgatgat ggacgtctat
    10501 gaggcagtcg agagccgacg ggcggtgcgg ggattcaccg accagcccgt gtcccgagag
    10561 gtgatcgaac gcgtgctgac cgccgcggct tggtcgccgt ccggatcgaa cattcagccg
    10621 tggaacatct acgtggtcac cggcgcgccg ctggccgagc tcaagaagct cgccgtcgag
    10681 cgcgtggcca ccggcgtccc gtgggacgag cgggaatacg agatgtaccc gcccgcaatg
    10741 aagtcgccgt acagcgaccg ccgtgccgcg ttcggcaagg agcgctacag cgcactcgga
    10801 atcgcacgcg aggactggga ggcgcgtcaa cgggcggcca tcgcgaactg ggactgcttc
    10861 ggcgcacctg cggccctgtt ctgctacatc gatcgcgaca tgggcccggc ccaatgggca
    10921 gacctcggca tgtatctgca aaccgtcatg ctgctgcttc gtgccgaagg cttggacagc
    10981 tgcccgcaga tggcgtggtc gcaggttcgt gagaccgtcg cggatgtcgt ctcacccgcc
    11041 gaagggcaga tcctcttctg cggcatgtcg atcggctacg aggacaagac ggttccctat
    11101 acccgtactg gccgggcgcc gctctacgag actgtcactt tcgtcgacgg ttagctcagg
    11161 acggcacgct ggccagcgcc tcggccacgc actcctgcca tcggtcagcg tcgactttct
    11221 cgatcgcggt gtctccgcca aaagcgctgg agatcacgat gttcggctgc accgtaccga
    11281 gcaactccag gctggacgtc aactctgcag cgctgccgcg accgggcacc aagaatgtcg
    11341 accaggtgcc gtcttcggtg ggaaacatcg tgtcgccggt gaacagatac gtgtcgccgt
    11401 cgactccccg cacgagataa caggtgctgc catccgaatg gcctggcgtg ggaatggcgt
    11461 cgacgccgtt ggcatcgagg tgccgcgaac cctctatggc cacatcgaca tgtgcgtggc
    11521 ggccgatcgc atccagctct gccgcgggcg catgcaaccg ggagccgaat cggtcagcga
    11581 tacgcgccag cgtcggaccc gcttcgtcga gatgcgacag atactgggcg ctcacgccgc
    11641 caaggtcgcc aatcccgtcg aaatccgcat cggacgccgg actgtagaac aacacgttgc
    11701 cgctcggacc tcgccacagg tacgcatgtg tagtcagtcc gggaaacggt gattctgtgc
    11761 gagtctgcca aaggtcgtct cggatttgcg tgatggtggt actcatgtct atacggtgct
    11821 tcctcaacca aagttgaggt caagccgtcg cgtggaggtg gtcgcgatgc aagcgattgc
    11881 accgatgatt gcttgtggac tcgctgcggc gtcgatcccg ctcgccggca ccgcctcagc
    11941 ggtctgcgac tcgcgggaat gcgtcccgaa cgtggccaga aatgtcgtgc agggcgggcc
    12001 ctgcgcgccc cggaagacgt tcgtcttcgg gctcgacgcg ggcggtgcta cgtacgtgtg
    12061 cactgtggct ggcgcgtggt ctccggtcgg cccgctgatc ggtgtgcgga acgtgccgat
    12121 gtcgtgtccg gcgctgaacc tgtcggccca gggctccgac ggcgtcccgt tctggtgcgt
    12181 ggacatgggt ggtggggcgc tgcagtgggc acaccgcccg gacaccgtcg gataggtcag
    12241 cgctcgacga cggaggcgat ccgcccggcc acatcggtcg cgacggccag aacctgcggg
    12301 tcatcggcag ccgcgtagcg gtccagggcg cggtcgtaag tggtgcccgc aattgacccg
    12361 tggtcggcgg gcagctcgac gaattcgacg ggccagtcgt tgtcgctgag cgtcgcggcg
    12421 aaggaccgac tcgcctcgac tggcacgaca tcgtcggcag caccgtgaag gagcgtgaac
    12481 ggtgagcgct gcgcgggcaa ttcggtcggc aagtgagcgg ccgtgatcgg atcttccgcc
    12541 atgaacgcac cggccaggca aaccgtatgt gtcacaggaa cttcgaactg tgcagcatgg
    12601 atcgccagcg ccgccgcggc cacaccgccc agcgaccacc cgaccaccac aaggccgccg
    12661 gcaccggcat gctgtcgcgc gaattctgcc gatcgcagca gatccccgcg gccgtggtcg
    12721 ttggcgtgtg agttccagtc aggcaccact acggtgaggc cgtggccggc caccagttgc
    12781 gcgagcggtc gcatcgcgct gcgggcatcg gattgggcgc cgtgccacat cagaactgtt
    12841 ggacccgcct tgtcgtcgaa caagtcaagc gttcggccgt gggtgtattc gactgtctgc
    12901 acacggcgag tctgcccacg cgacgacgta cctaaccgcg tgaccggtga tcctgcatgt
    12961 aggccgccca cctctgcgcc tcgctgggta gaggcagatg cgtgtggccg cattccagga
    13021 gccaggcacc gggaccggcg gtgcgggtca accggaaacg attgcagtac cgacagtgcg
    13081 ccatgactcc atctcctttc gtaaaggggt tactcgtctt gtgtaagcac ggtatggagt
    13141 tgcgcgacct gccaaaacgg gtcgtagacc catgtgtcgc cggcccgccc ggggcatttc
    13201 cgggcgcgcg gcattggggg attagggggt gtggcggcgc aggccgcgcc gagagctact
    13261 tggtgccgaa gatgcggtcc ccggcatcgc cgagacccgg caggatgtag ccgtgctcgt
    13321 cgagctgccg gtcgatcgcg gcggtataga tcggcacatc cgggtgggcg ttctgcatgg
    13381 cggcgatgcc ctcgggacag gtcagcaagc agacgaactt gaccgacttg ggaccacatt
    13441 ccttcagccg gtccaccgcc gcgaccgccg agttaccggt ggccagcatg gggtcgacca
    13501 ccaccacgtc acgctcgtgc agctcgccgg gcatcttgaa gtaatactcc acggcgacaa
    13561 gcgttttggg gtcgcgatac aggccgatat ggccaacgcg ggcgccgggc accaccgtca
    13621 gcatgccgtc cagaatgccg ctgcctgccc gcaggatgga cacgaacacc agcttcttac
    13681 cgtcgatgac ccggccggtg gtgctttcca gcggagtatc gatctcgacg tcgtgcagcg
    13741 gcacgtcccg aagcacctcg taggccatca gggccgagat ctcatgcagc agttggcgaa
    13801 agctgttggt ggacgcgtct ttccggcgca tcaaagtcag cttgtgctgc accaaggggt
    13861 gctcgatcag atggacggtg gccatggccg ctagaagacc gtgccgtcac tgacgacaga
    13921 aatgggtgga cgtccgccgc ggaagcgctc ggcgagcgca cggcccgccg cccaggttcc
    13981 accctcgagc acacacgcga gcggcatctg ttcagcatcg gtgccaaacc gggcgcgcac
    14041 cagcggcgcc agctcgtcga gcaacgccac cgtcaaggcg cgccactcga cgaccaattc
    14101 actgcccacc gtccatgtgc gctccgacca ggacgggtcg tgcaagcgca acacgtcggc
    14161 gtcgagcagt aggccgccgt tgcggtactc gggaagtccg gtgagagcgt cctggccttg
    14221 cacgtacacg ccagcccatt gaaaaggctc cagcagcgag taggtcagcc actgcgacaa
    14281 cttgtggaac ggcacccagc cccgggtcag gcccgaaccg gtgacggcct ggtggcgcca
    14341 gcaatcaccg agcgggtggt cgccaatggc gttgtgggtc ggccaaattc ccgacaacga
    14401 gagcaggaga tgcgacagga tgtcgtgcgc ggtcacgggg cctggatgct cgaccaacaa
    14461 gtcgaagagc tcacctggcc gaccatgcgt accaaacagc tcacgctgcg cggtcagcgc
    14521 ctcgcctagc cggtgcagca gagccatgcg accttcgaag cccaccaagg gattggacgg
    14581 gctgatttgg aaggcctcgg ccagcttgcc cgcgtcgagg gcacgcagtc ctgcggcgtc
    14641 gacccgaagc gggtgcgcgg catcgctgga gaacagtccg cccaggaagg cgtgccagct
    14701 cgcgactgcg agcccctcag agcgtgagaa gcgttgccca ctatccgttt ccaggtaccg
    14761 ccagtccggg ccagcgccgg catcgagcag cacgctcacc gtggtcaggt caatcatggc
    14821 gcgggcacgg gccggcgtat ccagctccgc cagtcggtcg tccagctctg ccttgcggtc
    14881 cacgccgccg gcgtcgaaat ggcgccatcg gctgtggtac gggatgctca ggtcggggta
    14941 gcgcgtgcgc atcatgtcgg ccacctcgac cgcggccgcc tgcagcgcat cgtcatgcac
    15001 gctgaaccag cgtgaggcgc cgtctcgtgc acgatgcagc aactggcggg catgtgcccg
    15061 cacgaccgtc gtggtacgca gtgccgcggc ggcgccctcg ggggtgtcgg cgttcaagtc
    15121 gggatgcatg gtgatgaggt tcacttctcg aactcccggc ccttgggctt actcaattca
    15181 tcggcgtcgg gcaccgggcc aggagtgaag tacccggccg ccatcttggc gtcgatctcg
    15241 acgcgcgcat cgggcgggat cagcgcatcc ggaatgttca cccgttcacc gacctcgatt
    15301 ccggaagcgg tgatcgcgtc gtacttcatg ttgctcatcg acacgaggcg gtgaatcttg
    15361 cggattccca gccagtgcag cacgtcgggc atcaactcct ggaagcgcat gtcctgcaca
    15421 ccggcgacgc actcggtgcg ggcgaagtag gcctcggcag tgtcgccacc cacctgtcgc
    15481 ttgcgcgcgt tgtagaccag gaacttggtc acctcaccca atgcgcgacc ctctttacgg
    15541 gagtaggcca ccagacccac gccgccccgc tgagccccca ggatgcactc ctcgatcgca
    15601 tgcgtgagat aagggcgaca cgtgcagatg tccgaaccga acacatccga gccgttgcat
    15661 tcgtcatgca cgcgtgcggt caattccaca gagggatcgc cgaggtcgcc gccgttgccg
    15721 aagatataga tggtctgccc gccgatcggc ggcaggaaga cctcgaggtc cgaccgtgtg
    15781 acgagttcgg ggtacatgcc accggtctct tcgaacagca cacggcgcag atcgttctcg
    15841 ctgcaaccga atcggtgcgc cacgcccggc agatgccaaa caggttcgat cgccgccttg
    15901 gtcaccagcg ccgcgccgtt gggcagcagg attcggccgt cgggcaccaa ccgccctttc
    15961 gctacggcgt ccatgatctc cggcacgatc acatgcgcct tggtgatcgc gatggtcgga
    16021 cggatgtcat agccggcggc cagttcggtg gcgaacacgt cggccaccat cgcgccccac
    16081 gggtcgaggc tgacgatctt gccgggctcg ctccactgcg ggaacgggcc gatgacatcc
    16141 gtgggcgacg tgtccgtgag gtcggccttg tgctcgcgcg acaacgcgcc cgccgccacc
    16201 gccagcgccc ggtacacgct gtacgacccg ctatgcgtgc cgatcacgtt gcgatgcgca
    16261 cgcttggtgg tggtgccgac gagcggacct cgctcggctg ccgtcggcgc accccaacga
    16321 atcggcggag catcgaggcc gccgctgtgt gacgtgaggc ggatatggcc ggaggcgctg
    16381 ttcgacttcg cacgcgacgt cgcggcggcg ccgggctcag cagacatggt cgtcctccgt
    16441 caagaaggcg caagcatatc cactctgccc tctcgacgct gttcctgcag gagcgccaca
    16501 agcgccaatc gaagcagcaa acacaagtca gtcacgccgc gatccggcgg aaaatagaac
    16561 gcgttacgat cgcttcatgc tgcgggggga actccctagt cggtttgact acctgttcga
    16621 gccgctggag gccgacgagc cggccggtaa cggcggcctc ccgaaatcct cttgcggccc
    16681 gaggaggaca accgctggtc caaccgaatg gtgcacgccg gcgtcgttct agcaacgctc
    16741 gcggccgccg ctgccatggc catcgtgttg ctccagccgg cccggccagc ggaccaaatc
    16801 gtcacgccga gtgacgccac gtcacccgcg acagcgacgt cgccagcgat gtctacaccc
    16861 atgccgacga caaccacagc acccgttccc gtcctaccgg cgacggtcag cactgccccg
    16921 gctcggaccc ctgaacagac accgaccgtg cagcggcaac agccggcggt ccagccggcg
    16981 ccgacgacaa ttgccagcga accacccgcc ccgatgccgc cgccgccgac gacacgcgcg
    17041 cccatcagtg tcagccccga aacacgtgcg ccgtttccga atcaggcgcc gtctcgaaac
    17101 aacgatcagc gcagcggcgg cggcggattg ctcggcggcc tgctttagct gacggacccc
    17161 gacacgccag ttcgcaggaa ccacacagct ttccgtgcgc ggcatccggg ctatgtcgct
    17221 gctattctgc caacgtcgca gcggcatgac ccgaacgaaa cagcagagtc cctcacgaaa
    17281 agagttttgt gcgcaccgga gagatcaagg ccctgtccgg cttgcgcatc gtcgccgcgg
    17341 tgtgggtggt gttgtttcat ttccggccat ggctcgaggt atcggcgccc ggcttcaggt
    17401 ctgctctcgc ccccgtgctc aattgcggtg cacaaggcgt cgacctgttc ttcatgctca
    17461 gcgggttcgt gctgacctgg aactacctcg accgcatggg ggagtcgttc tcgatccggt
    17521 cgacgctgca tttcttgtgg ttgcggctgg ccagggtatg gccggtctat ctcgtcacgc
    17581 tgcacctcgc gctcgcgtgg atcatcttca cgctttacgt cggtcacttg ccgtcgccgg
    17641 tcgccgacca gctcaacgcg gtcagctatg tccgccaggt cctgctggtg caactctggt
    17701 accagccgtt cttcgacggg tcgagctggg acggccccgc ctggtcgatc agcgcggaat
    17761 ggctggcgta cctgttgttc ggggtgttgg tgctggcggt cttccggatc tcccgggcga
    17821 caagggcaag aggcctgctg tggctggcaa ttgtggcctc gctgccgccc gcgctgcttc
    17881 tgctggccac cggccatttc tacacgccgt ggagctggct tccgcgaatc gttatgcagt
    17941 tcaccgctgg ggccctggcc tgcgccgcgg ttcgcaaatt gcagccgact gaccgggccc
    18001 gccgcatcgc cggttatctg tccctcgcgc tcggcatcgg catcgtcggc attctctatt
    18061 tcctggatgc gcatacggtg ccgaaggtct ccgactccag cggcctcgtc gacgtgctgt
    18121 tcgtgcccct ggttgtcacg ttggcgatcg gagtcggcag tctgccgcgg ctactctcca
    18181 cccgaatcat ggtgtacggc ggatacatct cgttcagcct gtacatggtg cacgagttgg
    18241 tgcacaccgc ctggaactgg ttcacagcgc aattcgcgat cgtcctggaa ccgagctggt
    18301 cggcaaaatt gatcgtggtt ggtgtgctgg gagtcgccgt cctcggcgcc gcggcgctgt
    18361 atcacttcgt cgaggagccc gcccggatct ggatgcgcaa catggtcaag gtccgagaca
    18421 cccaacccgt cgcgcgcgac gcaccggccg aagctcgtac cgaggccgcc gatccggcac
    18481 acagcaagct ccaacctctg cacggcccgc ggcatgtgcc ggcgaaagcc gtcgccgccc
    18541 gcgcgggctg accggtggcg gagagccgaa acctactgcg cccgatggca aagcagcgat
    18601 tctgtaacgc attgcgcaca atccggtcga caccggcgtt ttcaagagtt ggcccgtaag
    18661 ctgttccggt gagtcgtctg gtcacgctct tcgtcgcgat cgctgtgctt gtcggcctcc
    18721 tcgacgcgag gtcaacaccg gtgaggcaca acgacataat cacgttggag tcgtcgctca
    18781 accacatcgc ggtggtcggg gactcctaca cgaccggcgg cgagctgggc ggtcttggca
    18841 ccaagggatg gaccaccggg gcctggcagt tattggctcg gcagggcata gccgtcaccg
    18901 cggacgttgc cgccgagggc ggggcagggt atggcacgcg cggcaaccac ggcagcgtgt
    18961 tcgaggacct caccgccgac gcggtcaaac cagacgatgc gttggtggtg ttcttcggct
    19021 cccgcaacga ccaaggcgtc gacccgacac agctgtcgat cctggcgtgg ggcaccttcc
    19081 aattggcgcg gcgcacagcg ccatccgcga aattcctggt gatcggcccg ccatggccaa
    19141 cggccgaccc ccccgatgcc gtgctcaaga tccgcgacac cctgaagtat caggcggacg
    19201 tcgtcggcgc gacgttcatt gatccgatca acgcaggctg gttcgtcggc aggcccgagt
    19261 tgatcggcaa ggacggcgtc catccgaccg acgcaggcca tgcctacatg gcggagaaga
    19321 tcgcaccgct gatcggcgca caactgactc gaaagacctg acaatgcacg acgccccgcg
    19381 agccgattga cccgcggggc gccgtaatta gtcgatcagg ccaggtcgaa cctgtcgttg
    19441 ttcatcacct tgacccacgc cttgacgaag tccgccacga acttctcctt ggcgtcgtcg
    19501 gtcgcataga cctctgcgag cgagcgcagg atcgagtttg acccgaacac gaggtcgttg
    19561 gccgtggcgg tccacttcgc cgccccggtc tggcggtcgc ggccctcgta cacgttctcc
    19621 gtcgactggg acacatccca caccgtgccc atgtcgagca ggttgacgaa gaagtcgttg
    19681 gtcagcacgc ctggcttgtc ggtgaagacg ccgtgcttgc tcttcccgtt gcccagtaca
    19741 cgcaggccgc cgatgagaac cgtcaactcc ggagccgtca ggttcagcag gtaggccttc
    19801 tccaccagca ggcgctccac cggagccttc tcgccggcgc ggatgtagtt gcggaatccg
    19861 tcggcccgcg gttcgagaac cgcgaacgcc tcgacatcgg tctgctcctg cgaggcgtcg
    19921 gtgcgtcccg gcgcgaacgg caccgtgatc tcaacaccgg cgtccttcgc cgccttctca
    19981 acggctgcag agccgcccag cacgatcaga tcggcgaggg agaccttctt gccgaactcc
    20041 gactggatct tctccagggc gggcaacacc gtgtccagat cggcggtctt gttcgcctcc
    20101 caatccttct gcggcgcaag gcgaatccgc gcgccgttgg ccccgccacg caggtcggtg
    20161 ccgcggaagc tggacgccga cgcccacgcc gccgaaacca gctgggagac agacagaccc
    20221 gagtcgagaa tcttggcctt gagcgccttg gcgtcctcgt cggtgatcag ctcgtggtcg
    20281 acagccggga ccgggtcctg ccacagctgc ggctccgcca cccaggggcc gaggtagcgc
    20341 gagatcggtc ccatgtcgcg gtgcagcagc ttgtaccacg cccgcgcgaa cgcgtcggtc
    20401 atctcctcag ggtgatccag ccagcgacgg gtgatcttct cgtaggccgg gtccatccgc
    20461 agcgacaggt ccgtcgtcag catcgtcgga tgggtcttgc cgttgccgaa cgcctccggc
    20521 accgtgtccg cgcccgcgcc gtccttcggc cgccattggt tggcacccgc agggctctgg
    20581 aacagctccc actcgtgggc gtagagcgtc tcgaggaagc tgttgtccca agtggtcggc
    20641 gtcgccgtcc aaatgccctc gaggccactg gtaatggcgt cctttccgac gcctgtgcct
    20701 tgcggattca cccaaccgag gccctggaac tgcagggggc agccttccgg ttcgggaccg
    20761 acgaggtcgg cattgcccgc gccgtgggtc ttgccgaccg agtggccgcc gacgatcagc
    20821 gcggcggtct cctcgtcgtt catcgccatc cggcggaacg tctcgcggat gtcccgcgcg
    20881 gcggccagcg gatccgggtt gccgttgggc ccttcgggat tgacgtagat caggcccatc
    20941 tgcacggcac cgaacggacc ctgcaattca cggtcgccgg cgtaccgctc gtcgccgagc
    21001 caggtgtcct cgggtcccca gaagatctcc tcgggctccc agatgtcctc gcggccgaag
    21061 ccgaagccga acgtcttcag gcccatcgac tcgatggcgc agtcgccggc gaacacgagc
    21121 aggtcggccc acgagatctt gcggccgtac ttcttcttga ccggccacag caggcggcgg
    21181 gccttgtcga ggctgacgtt gtcgggccag ctgttcagcg gggcgaagcg ctgcgcgccc
    21241 tcgccgccgc ccccccggcc gtcggcgatg cggtacgtgc cggcggcatg ccacgacatg
    21301 cggatgaaca gggggccgta gtggccgtag tcggcgggcc accactcctg cgacgtggtc
    21361 atcacgtcga tgacgtcctg cttcagcgcc ccgaggtcga cggtcgcgaa ctccttcgcg
    21421 tagtcgaagt cctcgtccat cgggttgccc cgcggcgagt gctggtgcag gacctggagg
    21481 tcgagctggt tcggccacca gtcctggttg ctcatggggc ggcccggctg cttcgacgtc
    21541 ggggccttga tcgctgggtt ctcgctctcg ctcacgtcgg tcctccgggg gcggctcgaa
    21601 acttagactc actctaaagt tctt
//
LOCUS       DASRJA010000620         6546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1767749,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000620 DASRJA010000000
VERSION     DASRJA010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767749"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFC01_27945"
     CDS             complement(<1..613)
                     /locus_tag="VFC01_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN83486.1"
                     /translation="MAKPMRGGVQLRGVSKTYGEGGAAVHALREVDLDIPAGALVAIL
                     GPSGSGKTTLLNIIGGIESADCGTIVVAGQDISGRPPRDLGAFRRAHVGFVFQFFNLI
                     STLTAYENVQVVMELTRRGDRGHVPDLLAAVGLSDRAGHFPAQLSGGEQQRVSIARSL
                     ATDPDLLLADEPAGALDVATGRRVLELLQQTGRAGRGVIMVTHN"
     gene            complement(893..1387)
                     /locus_tag="VFC01_27950"
     CDS             complement(893..1387)
                     /locus_tag="VFC01_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HZN83487.1"
                     /translation="MNTSPFVILTSLAALFSAAAGGMMYTFSTFVMRGLDRTGPVDAI
                     TAMRGINAEANANPAFLLGYFGATILALIVGVMAVVKLNQPGSWLVLVGAVFALLGAV
                     ITMVFNVPLNNHLDTVNPDGLSVADAAREWQAYFSTWTAWNHARTATSFVGALLMLVG
                     LRYR"
     gene            complement(1546..2238)
                     /locus_tag="VFC01_27955"
     CDS             complement(1546..2238)
                     /locus_tag="VFC01_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HZN83488.1"
                     /translation="MTTTDCPPLPVRITKSLLGYGVIAGPTYVLAVAAQMATRDGFDP
                     TRHAASQLANGDFGWMQVATFVVTGAMTIAAAVGIRRALGAGRLSAWASGLLGGYGAG
                     LVIAGMFRADPSDGFPPGTPPGMGEVSWHGLAHFAVAGIAFACLVAACFVLGAWFARR
                     GLGSWAWFSRITGVAFAASFMALSSGSGGATTILVFTAAVALVWAWLSAVSVKLYRSV
                     ASTPISTSTTTP"
     gene            2329..2964
                     /locus_tag="VFC01_27960"
     CDS             2329..2964
                     /locus_tag="VFC01_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN83489.1"
                     /translation="MTEAVLGRSARKRLTILSAGRDLFLSNGYQGTSVDQIAASAEVS
                     KQTVYKHFGDKHELLLAIVNTALESTVTPFLERIRALADTADLEADLTALAADYLRAV
                     LQEPVVQLRRLVVGEANRVPELAQLYYEHAPARTLAAFADCFSVLHDRGLLRVTESAV
                     AAEHFAFLIVGRSIDQALFYGGPQVLAGIDVDGDVRAGVQVFLAAYQARKG"
     gene            2975..3940
                     /locus_tag="VFC01_27965"
     CDS             2975..3940
                     /locus_tag="VFC01_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZN83490.1"
                     /translation="MDAVVGLLDGVRARGAFVLRMMMDPPWSMSIKDDAPLTLICQTH
                     GRAAIVGESSGTTWLGPGDVALTRGTEHYVFADDPATAPMVVIHPGNRCTTLSGESLK
                     FEMSLGVRTWGNSPSGATRSVICAYEGRSEVSTRLLDALPMVLVLRADDWETPLVDLL
                     AAEASHEGPGQEAYLDRLLDLLLIAVLRTWFDRDENAPTWWHAEHDPIVGPALKLIYN
                     NPAHPWTVANLAAAVGTSRAVFASRFTEQVGEPPIAFLTNWRLALAADLLRSSESTIA
                     AVARQVGYSTPFALSSAFKRSYGVSPNTYRANAAYADATLDSSAL"
     gene            complement(4331..5494)
                     /locus_tag="VFC01_27970"
     CDS             complement(4331..5494)
                     /locus_tag="VFC01_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZN83491.1"
                     /translation="MSGFGFDTLALLAVIGMAGPLLASVPRFRVPVVIGELTAGLVVG
                     RTGFGIVDHTDPTFTLLANIGFALVMFVVGTHVPVRDKTLRSEIPTSLLRAVAVGAVA
                     AVVGVVLAQAFGTGHAAVYAVLMASSSAALALPVIDSLRLEGRPVLSVTTQIAIADAT
                     SIVLLPLVINPAQAPRAALGGLAVAGAAVLLYFLLRTATRRGWRRRMHHYSRTRRLAL
                     ELRLSLITLFTLCALAVSTHVSIMLAGFALGLVVSAIGEPRRLARQLFGITEGFFAPL
                     FFVWLGASLQVRELGEHPLLILLGVGLGVGAVLAHSVGRLFGQPLTLAVLSAAQLGVP
                     VAAATLGTEEHLLAPGESSALMLGALVTVTTTSIVGVLAARRQSGDEASTPSE"
     gene            complement(5495..5869)
                     /locus_tag="VFC01_27975"
     CDS             complement(5495..5869)
                     /locus_tag="VFC01_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83492.1"
                     /translation="MAVKWLDEPEVHDYDAAAAYLSMLAEKDAVDKTVAALKSAKPVF
                     QKAKDILRAAGLQLLPETNAHVRSDLGKISDGKKLSPILLVRGSAVGAYPLQIADGYH
                     RVCASYLTDENTNIPCHLVSWQ"
     gene            6239..>6546
                     /locus_tag="VFC01_27980"
     CDS             6239..>6546
                     /locus_tag="VFC01_27980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83493.1"
                     /translation="MRRFAIATLLVVATSAPAVAGAALIDHSTIAMLGQPGGCSTSCD
                     VGGKGTGGYQKEPDTGFGSFTQAGTPGTGRITLNNPPDQVGTQSGNYPKGAGHCTGYL
                     T"
BASE COUNT         1196 a         2200 c         2097 g         1053 t
ORIGIN      
        1 cgttgtgagt caccatgatc acgccgcgtc cggcgcggcc ggtctgctgc aacaactcca
       61 gaacccgacg cccagtcgct acatccaatg cgcccgccgg ttcgtcggcc agcagcaggt
      121 cggggtcggt ggccagcgag cgggcgatcg agacccgctg ctgttcgccg cccgacagct
      181 gcgccgggaa atgaccggca cgatccgaca agcccactgc ggcaagcaga tctggcacgt
      241 ggccacgatc cccccgcctc gtcagctcca tcaccacttg cacgttctcg tatgcggtga
      301 gagtggaaat cagattgaag aattggaaca caaacccgac atgtgcccga cggaatgcgc
      361 ccagatcgcg cggtgggcgc cccgatatat cctgccccgc aaccacgatc gtgccgcaat
      421 cggccgattc gatgccaccg atgatgttga gcagtgtggt cttgcccgat ccgctcgggc
      481 ccagtatcgc taccaaggcg cccgcgggga tgtcgagatc gacctcccgc agggcgtgca
      541 cggcagcccc gccctcgccg taggtcttgc tgaccccgcg taactggact ccgccgcgca
      601 tcggtttcgc catcgcgacc tcccgcgttc ttgtcaggct agtccggcga gcagctgatc
      661 gaccccctac tcggggtccg agaaaccggc ctggccgcgg cacgagcagg ccggagagtc
      721 gcggactggg cggcgctcac gtggcggcga ccaactcgca tcctcgttga gttccgcccc
      781 aacctgtcgg gcggtcatac cgtaggtacg cggcggagcg catgacggtt caccgaatct
      841 tcggcctgtg gcgctgccag tcggaatgac cggcctggtg gcgcggggag tatcaccggt
      901 accgcagccc aaccagcatc agcaaggcgc ccacgaagct ggtggcggtg cgggcatgat
      961 tccatgccgt ccacgtcgag aagtacgcct gccactcgcg ggcggcgtcg gccaccgaca
     1021 ggccatcggg gttcacggtg tcgaggtggt tgttgagtgg cacgttgaag accatcgtga
     1081 tgacggcgcc gaggagggcg aacaccgctc cgaccaacac cagccagctg ccgggctgat
     1141 tcagcttcac cactgccata acgccgacga tcagggccag gatcgtcgcg ccgaagtacc
     1201 ccaggagaaa cgccggattg gcgttcgcct cagcgttgat gccacgcatc gcggtaatcg
     1261 catcgacagg tccagtgcgg tccaggccgc gcatcacgaa ggtggagaac gtgtacatca
     1321 ttccgccggc agcggccgag aacagcgcgg cgagggaggt caggatcaca aatgggcttg
     1381 tattcatgac cagaagtcaa tcgggaaccg cgaagacgat ccatcgttga ccgtcccgac
     1441 cgcatacgcg accgtccggg tggctaccca accggcatgc gccgtctttg agtgctcacc
     1501 ctcaacgtcg ccggatcgcg cccgacctcg tgtccttcca ggaggctacg gcgtcgtcgt
     1561 cgacgtcgaa atcggcgttg acgccaccga ccggtatagc ttcacggaca ccgcgctgag
     1621 ccaggcccac accagcgcga cggctgcggt gaacaccaga atcgttgtcg ctccaccaga
     1681 tccggatgac agcgccatga agctggcggc gaacgccacg ccggtgatgc gggaaaacca
     1741 tgcccacgaa cccaatccgc gacgggcgaa ccatgcgccg agcacgaagc atgccgctac
     1801 caggcacgcg aatgcgatgc ccgccaccgc gaaatgggcc agaccatgcc agctgacctc
     1861 gcccataccg ggcggcgtgc ccggcgggaa tccatccgac gggtcggccc ggaacatgcc
     1921 tgcgatgacg agaccggcgc cgtagccccc gagcagtccg gatgcccagg ccgacaggcg
     1981 tcccgccccg agcgcgcgcc ggattccgac ggccgccgcg atcgtcatcg caccggtgac
     2041 cacgaacgtg gcgacctgca tccacccgaa gtcgccgttc gcgagctggc tggccgcgtg
     2101 ccgggtcggg tcgaacccgt cgcgggtcgc catctgggcc gccaccgcca gcacgtaggt
     2161 ggggccggcg atgacgccgt agccgagcaa cgacttggtg atccgcaccg gaagcggcgg
     2221 acaatcggtc gtggtcatca cgggcctttc gctcgaatca aactagactg tccagtaccc
     2281 tagccgtgtt ggaactagac tgtctagtac tgcattgagg agggccgcat gaccgaggct
     2341 gtgctgggcc gttccgcgcg taagcgcctg accattctgt cggcgggccg cgatctgttt
     2401 ctgagcaacg gctaccaggg caccagtgtc gatcagatcg cggcgtccgc agaggtgtcc
     2461 aagcagacgg tctataagca tttcggtgac aagcacgaac tgctgctggc gatcgtgaac
     2521 accgcgctgg agagcactgt gacaccgttt ctcgagcgga ttagggcact cgccgacacc
     2581 gccgacctcg aagccgatct gaccgcgctg gccgccgact acctgcgcgc ggtcctgcaa
     2641 gagccggtgg tgcagttgcg acgactggtc gtcggcgagg ccaaccgggt tccagagttg
     2701 gcgcagctgt actacgagca cgcgccggcc cgcacgctgg ccgcgttcgc cgactgcttt
     2761 tccgtgctgc atgaccgggg gctgctgcgt gtcaccgaat ctgcggtcgc cgcagagcat
     2821 ttcgcgttcc tgatcgtcgg ccgatcgatc gatcaggcct tgttctacgg ggggccgcag
     2881 gtgctggcgg gtatcgacgt cgacggcgac gtccgcgcgg gggtccaggt ctttctcgcc
     2941 gcttatcagg cacgcaaggg ataagctccc gggcgtggac gccgtggtcg gcctgctcga
     3001 cggtgtgcga gcgcgaggcg cattcgtatt gcggatgatg atggatccgc cgtggtcgat
     3061 gagcatcaag gacgacgccc cgttgacgct gatctgccag acccacggcc gcgcggcaat
     3121 cgtcggtgag agcagcggca ctacatggct ggggcccggc gacgtcgcgc tgacccgtgg
     3181 caccgagcac tacgtcttcg ctgacgaccc ggccaccgcg ccgatggtcg tcatccatcc
     3241 tggcaaccgc tgcaccacgc tgagcggcga aagcctgaag ttcgagatgt cgctcggggt
     3301 gcggacgtgg ggcaacagcc cgtccggcgc gacgcgctcg gtgatctgcg cctacgaggg
     3361 ccgcagcgag gtgagcacgc gtctgctcga cgcgttgccc atggtgctcg tgctgcgggc
     3421 cgacgactgg gagaccccgc tggtggatct gctcgccgcc gaggcaagtc acgaaggtcc
     3481 cggccaggag gcatacctcg accggcttct cgacctgttg ctcatcgctg tgctgcgcac
     3541 ctggttcgac cgcgacgaga acgccccgac gtggtggcac gccgaacacg acccgatcgt
     3601 cggtcccgca ctgaagctga tctacaacaa tccggcacac ccgtggaccg tcgcgaatct
     3661 ggctgcggca gtgggaactt cgcgcgccgt gttcgccagc aggttcaccg aacaggtcgg
     3721 cgaaccaccc atcgcattcc tgaccaactg gcggctggcg ctggccgccg acctgctgcg
     3781 ctccagtgag tccacgatcg ccgcagtggc ccggcaggtc ggttacagca cgccgttcgc
     3841 cctgagcagt gcattcaaac ggtcctacgg tgtcagcccg aacacctacc gcgcgaatgc
     3901 cgcctatgcg gacgccacct tggactcctc ggcgctctga cgcagggcgg cgagtccaac
     3961 gcgtccgttc gggtctagcg ccgccaccac cgactttgct aacgtcgcac ttgttgaaac
     4021 cgcaacccgg tcggggtgca gtcggccgct ctaagccgct ggctcacaga agcatccgct
     4081 ggtgccgacc actctgatga ctttcgccta acggcacggc catgtactgg ctgcacaccc
     4141 gtaactcagc ttcaacccgc cggcggcgca cgcggggcga ggggttccgc gcttgccacc
     4201 gggacgcgca gacatcaggc ttgcattggg cggagaccgt ggctgggggt cggatgtctt
     4261 ttgcaaatgg cgatgagggg gcctggcctc ggcgttcagc agcgaagcgt ctgcctctca
     4321 tcgccgaccc ctactccgaa ggggtgctcg cctcgtcacc gctctgacgc cgcgcggcga
     4381 gcactccgac gatcgatgtg gttgtgacag ttaccagcgc gccaagcatc aatgccgacg
     4441 attcgccggg tgcgagcaaa tgctcctcgg tgcccaacgt cgcggcggcc accggaaccc
     4501 ccagttgcgc ggccgatagc accgccagcg tcagcggctg accgaacaaa cggccgacgc
     4561 tgtgggccag caccgcgccc acgccgaggc ccacgccaag aaggatcagc agggggtgct
     4621 cgccgagctc gcgcacttgc agcgacgcgc ccagccagac gaagaacagc ggcgcgaaga
     4681 acccttcggt gatgccgaac agctgacgtg ccaggcggcg cggttcgccg atcgccgaaa
     4741 caaccaggcc gagagcgaag ccggccagca tgatcgacac gtgcgtgctg acagccagtg
     4801 cgcacagcgt gaagagggtg atcaggctca gccgcagttc cagcgcgagt cggcgcgtgc
     4861 gtgagtagtg atgcatccgc ctgcgccatc cacgtcgggt ggcggtgcgc agcaggaagt
     4921 acaacagcac cgcggcaccc gcgaccgcca gcccaccgag cgccgcccga ggagcctggg
     4981 cggggttgat gaccaagggc aacaacacaa tcgaggtcgc atcggcgatc gcgatctgcg
     5041 ttgttaccga cagcactggc cgaccttcca accgcaacga gtcgatcacc gggagcgcta
     5101 gcgccgccga cgacgacgcc atcagcacgg cgtacaccgc cgcatgtccg gtgccgaacg
     5161 cctgggccag cacaacgccg acgacggccg ccacagctcc gaccgcgacc gccctgagca
     5221 acgacgtcgg aatctcggaa cgcaatgtct tgtcgcgcac cgggacatgg gtgccgacga
     5281 cgaacatcac caatgcgaat ccgatgttcg ccagcagcgt gaacgtcgga tcggtgtggt
     5341 cgacgatccc aaagccggtc ctgccgacga ccagccccgc tgtcaactct ccgatcacca
     5401 cggggacgcg aaagcgcggc accgaagcca gcagcggtcc ggccatgccg atgacggcca
     5461 gcagcgccag cgtgtcgaag ccgaacccgg acatctactg ccaggacacc aaatgacaag
     5521 ggatgttggt gttttcgtcg gtgaggtagc tggcgcacac tcggtgatag ccgtcggcga
     5581 tctgcagagg ataagcgccg accgcgctgc cgcgcaccag aaggatcggt gagagtttct
     5641 tcccgtcgct gatcttgccc aggtccgacc ggacgtgagc gttggtctcc ggcagcagct
     5701 gcagtccggc ggcgcgcagg atgtccttgg ctttttgaaa caccggcttc gcggacttca
     5761 gcgccgccac ggtcttgtcg accgcgtcct tctccgcgag catgctgagg taggccgcag
     5821 ccgcgtcgta gtcgtgaacc tcaggttcgt ccagccattt caccgccatg gccggacacg
     5881 atactcagcc agcgatccag ttgccgtgga acttcttcca gcatgtttgg caacgttggt
     5941 tgcgattgat caggagagta atcacaggtg gtgggcgttg actgcgttgg cggcaaaggg
     6001 caacccgtga acctgctgcg gacgcactgc ggacggaacc caacggcccg ccgatgcgtc
     6061 gtacctatgt cggcccgcca cctgctctcc tggtggcggg ccgattgctg cggagtggcg
     6121 aacgtgcgtg cacgctttca ctggtagttt cctgcaaatt tcccttcggt ggcgatgtgg
     6181 tgcgcgtacc gtccgattga cgtggccgat atttttcgct gatcagagag gatcaaccat
     6241 gcgtaggttt gctatcgcca cactattggt cgttgcaacg tcagcgcctg cggttgccgg
     6301 tgcagcactg atcgaccact ccacaatcgc gatgttgggc caacccggcg ggtgcagtac
     6361 ctcttgcgat gtgggcggca agggcacggg cggctaccaa aaagagcctg acactggatt
     6421 cggatccttt acccaggccg gcacccccgg taccggccgt atcaccctaa acaacccgcc
     6481 cgatcaagtt ggtacccaga gcggcaatta ccccaagggc gcaggacatt gcacgggata
     6541 cctcac
//
LOCUS       DASRJA010000621         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_926462,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000621 DASRJA010000000
VERSION     DASRJA010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926462"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VFC01_27985"
     CDS             complement(<1..719)
                     /locus_tag="VFC01_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN83494.1"
                     /translation="MESLPLPAPGLTANSITVTAVFSNALNLPAKAKVKLNGADIGEV
                     ESTHAHDFTALVTMRIRSDVPVRIGTTAQLRSATPLGDVFVALKPDPNQAPGTPLLHD
                     GDVIPVGSTTAAATIEEVLGSAALLINGGAIRRLVSIINGAGSALGGRGDKVAELLHQ
                     SGELISRLDARSAQINDALRSTSELAAALQAHQNTVNEALAAGGPATDVLADNTAALT
                     DLVEAIAEITVQLNRFPSLQGT"
     gene            complement(809..1945)
                     /locus_tag="VFC01_27990"
     CDS             complement(809..1945)
                     /locus_tag="VFC01_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005078731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN83495.1"
                     /translation="MARLASTATQKAARMTRKIMIVAAALITVAVAVIVLIPHRVSRA
                     ITVTAQFEDSVGLYVGNAVSVLGMPVGKVTSIVPNGSYVEVRLEIGDGVDLPADVQAV
                     TVSTSVLTDRHIELTPVYRDGPKLRNGDVVGLARTRTPVEFDRTLAMVDKLSLTLSGD
                     GQGHGPLADLVNIGDKITTGNGPNIKATLDELAQALRLGADNGTQTKKDIQAIATNLA
                     ELTQAAADNDSAIREFGSNIRQLSDILADEDLGTGNAGKQINKILNQAAGLLQENRDG
                     LKSTVADANTLTAAINDYRREIAEFFDVAPLAIDNVYNAIDANAGSLRIHFLPDKILF
                     NGQFAKEVCNLIGAKQLGCATGTLADYGPDFGIPGMLEVMAGTR"
     gene            complement(1900..>2454)
                     /locus_tag="VFC01_27995"
     CDS             complement(1900..>2454)
                     /locus_tag="VFC01_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN83496.1"
                     /translation="QALNNMRSLAAIVAARRDQIGSLLRSVDTITATIRDHKANLGAL
                     VLQGRDLLAEIVSRRAEVEKLFSGATALVNTTEAVLDDQPAINEMLASIRDFSRMLAE
                     HDALLRNTFQALPLPIRNLANATGSGTALDLNVPAGPLVDSWMCAISGRAKQFSLTDY
                     FTDCQPAPDPWPGWPPPQPKRLPG"
BASE COUNT          381 a          712 c          844 g          517 t
ORIGIN      
        1 gtgccctgca gtgagggaaa tctgttgagc tgcacggtga tttcggcgat cgcttcgact
       61 aaatcggtca gcgcagcggt gttatcggcg aggacgtccg tcgccgggcc gcccgcggcg
      121 agcgcttcgt tcaccgtgtt ctggtgtgcc tggagcgcgg cggcaagctc cgaggtactg
      181 cgcaaggcgt cgttgatctg cgccgatcgg gcgtcgagcc gtgatatcaa ttcgccggac
      241 tggtgcagca gctcggcgac cttgtcgccg cgcccgccga gcgccgagcc ggcgccgttg
      301 atgattgaaa ccagacgacg gatggcgccg ccgttgatca gcagcgccgc cgagccgagc
      361 acttcctcga ttgtcgcagc ggcggtggtg gagcccaccg ggatgacgtc gccgtcgtgc
      421 agcagcggtg tgccgggcgc ctggttcgga tccggcttga gcgcgacgaa cacatcaccc
      481 agtggggtgg ccgaccgcag ctgggcggtg gtgccgatgc gcaccggaac gtcggatctg
      541 atccgcatgg tcacgagcgc cgtgaagtcg tgggcgtggg tcgactcgac ctcgccgatg
      601 tcggcgccgt tgagtttgac cttggctttg gccggcaggt tcagcgcgtt agagaagacg
      661 gccgtcacgg tgatgctgtt ggccgtcagg ccaggcgccg gaagcggcaa gctttccaac
      721 ccggttccgc atccggccac gaccacggtt gcggcggccg ctaccgcgca tcggaatccg
      781 ccacgggcgg tagcacactg gcggttgatc accgcgtgcc cgccatcact tccagcattc
      841 cggggatgcc gaagtccggt ccgtagtcgg cgagtgtgcc tgtggcacat cccaattgtt
      901 ttgcccctat aaggttgcac acttctttgg cgaattgccc attgaagagg atcttgtcgg
      961 gcaggaagtg gatgcgcagc gacccggcat tggcgtcgat cgcgttgtag acgttgtcga
     1021 tggccagtgg ggcgacgtcg aagaattcgg cgatttcgcg acggtagtcg ttgattgccg
     1081 cggtgagggt gttggcatcg gcgacggtgc ttttcagtcc gtcccggttt tcctgcagca
     1141 ggcctgcggc ttgattcagg atcttgttga tctgttttcc cgcattaccg gtgccgagat
     1201 cttcatcggc cagaatgtcg ctgagctggc ggatgttgga gccgaattcg cgtatagcgc
     1261 tgtcgttgtc ggcggctgcc tgggtcaatt cggcgaggtt ggtcgcgatc gcttggatgt
     1321 ctttcttggt ttgtgtgccg ttgtccgcgc cgagccgcag cgcctgcgcc agctcatcca
     1381 gcgtcgcttt gatgttgggc ccgttgccgg tggtgatttt gtcaccgatg ttgaccaggt
     1441 cggccagggg cccgtggcct tgtccgtcac cgctaagagt gagtgacagc ttatccacca
     1501 tggccagcgt ccggtcgaac tcgactggcg tgcgtgtgcg ggccaagccc accacgtcgc
     1561 cgttcctcaa cttcggccca tcgcggtaca cgggggtgag ttcgatgtgc cggtcggtca
     1621 gcaccgaggt ggacaccgtg acggcttgaa catcggcggg aaggtcgaca ccgtcaccga
     1681 tctcgagtct cacctcgacg tagctgccgt tgggcacgat gctggtgact ttcccgaccg
     1741 gcatccccag caccgaaacg gcgtttccta catacaagcc gacactgtct tcaaactgcg
     1801 cggtgacggt tatcgcccgg ctgacgcgat gcgggatcaa cacgatcacc gcgacggcga
     1861 cagtgatcaa tgcggcggcg acgatcatga tcttgcgagt catccgggca gccttttggg
     1921 ttgcggtgga ggccagccgg gccatgggtc gggtgctggt tggcagtctg tgaagtagtc
     1981 ggtcaggctg aactgtttgg cgcgcccgct gattgcgcac atccatgagt cgacgagcgg
     2041 gcctgcgggg acattgaggt cgagtgctgt gccgctgccg gtcgcattgg ctaggttgcg
     2101 gatgggcagc ggcagggctt gaaacgtgtt ccgcaggagc gcgtcatgct cggcgagcat
     2161 ccgggagaaa tctcgtatgg acgcgagcat ttcgttgatg gctggttggt catcaagtac
     2221 tgcttctgtc gtgttgacca acgctgtggc gccggagaac agcttctcca cctcggcgcg
     2281 gcgggagacg atctccgcca agaggtctcg gccctgcaac accagtgcgc cgaggttggc
     2341 tttatgatcg cggatcgtcg cggtgatggt gtcgacgctg cgcagcagag agccgatttg
     2401 atcgcgccgc gcagcgacga tcgcggcaag cgatctcatg ttgttcagcg cttg
//
LOCUS       DASRJA010000622        12429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_359181,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000622 DASRJA010000000
VERSION     DASRJA010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12429
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359181"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..144
                     /locus_tag="VFC01_28000"
                     /pseudo
     CDS             49..144
                     /locus_tag="VFC01_28000"
                     /inference="COORDINATES: protein motif:HMM:NF028194.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(350..976)
                     /locus_tag="VFC01_28005"
     CDS             complement(350..976)
                     /locus_tag="VFC01_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN83497.1"
                     /translation="MTTSTETDITAADFAGCSDRMLGSELKPRNDRAAVKTVEGREAA
                     TVKGLYDEHAAALWRYACRLTGDPARAQDVVQETLLRAWQHPEVANDTERSARSWLFT
                     VARNMIIDESRSLRFRREVSALDISGAPERTCPDEVNAALDRMLIGEALARLTPDHRA
                     VVQRSYLQGRTTAQIAADLGIADGTVKSRLHYALRALRLALCEMGYTS"
     gene            1532..2362
                     /locus_tag="VFC01_28010"
     CDS             1532..2362
                     /locus_tag="VFC01_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN83498.1"
                     /translation="MGQFLTSRRHQLVRADLGLPPIAGRMTRGLRREEVAYLAGVSVT
                     WYTWLEQARDVTPSRQVVDSLARTLRLSHAEHVHFLALAGYSAPPPAEDPSPGTAPAH
                     VQRLLDALADYPALAIAPDWAILAWNAAYAGLYPNVATVAAADRNFLWLLFTDPYLRA
                     LMPDWEFTGMYNVASFRAQAGTRLGEPPFADLVSRLLQTSEAFRAAWESYGIETLPSR
                     QRIFRHPEVGDLHVEQHNLAPSGDPGLQVVMFTPIPPTDTAERLRRLHDMQSLRLSGA
                     "
     gene            2601..3320
                     /locus_tag="VFC01_28015"
     CDS             2601..3320
                     /locus_tag="VFC01_28015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83499.1"
                     /translation="MLLHPLGSTGAMSWASLADSLRAVGYRVAAPDLPGHGTSRETSF
                     TWESARATVRAAAEQFGPDKPVLIGLSLGAATAIYAAQREPASFAGLVLAGAGACWND
                     RCLRTGLTAAAAVGALLAAVGRREPLAHAAGFRGREIFIAARTADVAPAQLWRAARQL
                     TRFDLRRTPPPPIPCAVIVLTDDRRMPPGLQRALARYLDCPHVDVAADHDAPVCQTGR
                     FSDGVHAALVSLRPHGRGIPL"
     gene            3317..4495
                     /locus_tag="VFC01_28020"
     CDS             3317..4495
                     /locus_tag="VFC01_28020"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN83500.1"
                     /translation="MTTQVDDSLDAVVIGAGPAGLAVARELEHQHGIETLVVDRAAAP
                     AISWRTRYDNFRLNTTGFLSHLPGQRIPLTAGRWPTREDMVRYFDDYVSRQNITLELG
                     CEVNGIDRAGGGWRLDTSSGEIRTRAIVLATGNYRTPTIPCWPGLSAFTGEFVHSGSF
                     KNAWPFRDRDILVVGGGNSAADIAVQLANNGARRIWLSVRTPPHLVRRAIAGFPSDIF
                     LELFAWAPASAVDPIIGLSENLMWGDLSAYGFNRPPLGLKATVEQTGRIPTLADELID
                     AVRAGRVEVVAAVEAIESGRVILADGNSIPPGVIIAATGFSMDLDGLVGHLGVLDERG
                     KPRGGFASHIGDGMFAIGYGIPPNGPLRAIRLAATPLAREIAAHLAATHASQQVTSHS
                     "
     gene            4723..6063
                     /locus_tag="VFC01_28025"
     CDS             4723..6063
                     /locus_tag="VFC01_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN83501.1"
                     /translation="MGLVSVDKYYFRGAEGYETARRATVWNGLLPDRFPDVIVQAHDT
                     DDVVAAIRYARANGHKVGIRSGGHSWAASHLRDGGLLLDVSRLDHCTVDTDRMTADVG
                     PGKIANVFAAELDSQGLFFPTGHCEGIRLGGYLLQGGYGWHSKVLGPACENVLGLEVV
                     TADGDQIYCDPQNHPDLYWAARGSGPGFFGVVTSFKLRIHPRPSVLGTCLYMYPIELA
                     DEVFTWGRSISPEIDARVELQILTTRSFPAAGIDHPVITIASPVFAETEEEAAKALAA
                     LGTCPVVDQAMINVPYAPTNLASWYTAVMTNYPKGHRYIADNMWTSASAEELLPGIRK
                     IIETMPPHPSHFIFTGWNASPDRPDMAFSVEDEIYMALYTVWQDPADDERYRDWSGSN
                     MAAMSHLATGTALADENLGRRPAKFITDPNMARLDKVRSTYDPDGRFHSWMGRA"
     gene            6066..7274
                     /locus_tag="VFC01_28030"
     CDS             6066..7274
                     /locus_tag="VFC01_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN83502.1"
                     /translation="MAVPNALRILVIGAGVGGISIARGLLRDGHDVTVFEQRPDMRPG
                     GGAVTIWPNGSTVLEQLGVDMHGAGQLLSTVRIATSTGRPLVNIDATAIVHRLGGSVR
                     MVPRRALLDRLLEGFPVDHIRCNSRVVGAVGTRDGVRIEFEDGSSAEGDLLIGADGLH
                     SMVRDIVGAKPAKATGWCSWQGLIALPDIADKDVALQVIGEHGNLGLWPAGGCDLQWW
                     FDLRWSPDFVRPERPIEMIRANFAGWSDLVDQVLATLTEDDLAASPFPHFRHPIPRSP
                     RLGAMTLLGDAAHTMPPILAQGTNQALLDTMVLRKALSDFRKGPNCGNSDLASALRWY
                     EKTRRRRLTAVSWVTSRQMSQSESVLKPAAMISDSFGTWAMTTFLRSISHRRISAEVN
                     RDLSSRLIRT"
     gene            7307..8398
                     /locus_tag="VFC01_28035"
     CDS             7307..8398
                     /locus_tag="VFC01_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZN83503.1"
                     /translation="MSKTAKVPPAKLARVIEWTRHHLYRLHQRLTPAPAAMIELILAG
                     WTSQAITVAAQLGVADALADGPLTIDELATRVDANADALHRLLRALISRGVFRHRRDG
                     RFELNSLADTLRSDAPASMKSAARFYGSQEQRERWTLLVDSVRTGTSVVPALRGKSSF
                     DYFADEPELATLFNQTMTSVSEMTDVPVVAGYDFSAYPTIVDVGGGHGPLLATILAAT
                     PASRGVLYDLPSVVANAPNLLREHDVENRVRIEEGSFFDSVPDGGDAYVLKNIIHDWR
                     DEKALQILRNVRAAAGPRATVLLIEFVIPNHDRDFPGKWVDLEMLLNLGARERTAAEY
                     RDLLSQAGFRLTRVVQTAAPLSVVEARAA"
     gene            complement(8506..8805)
                     /locus_tag="VFC01_28040"
     CDS             complement(8506..8805)
                     /locus_tag="VFC01_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83504.1"
                     /translation="MKHLAITMTVGALVLTALGMPGAATAAPSGPSPVDDTVASLQAE
                     GYKVIVTRLSGAESSNCSVAAIRPGQQITQETPTGGRGNHARQVVSNTVYVDVRC"
     gene            complement(9145..11415)
                     /locus_tag="VFC01_28045"
     CDS             complement(9145..11415)
                     /locus_tag="VFC01_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN83505.1"
                     /translation="MLQRIALLAIAAPRRVIAVILLLMLAAGIFGIPVAKRLSSSGFQ
                     DPTSESARATQLLTDKFHQGDMQMLITVTAPDSANGRSARAVGLAIVDQLARSPNVTA
                     VASAWTAPPAAAERLVSKDGKAGLVVAGIGGGESVAQTTAQTLSDEIARDHPVGTNGV
                     IVRSGGDAMVNAQINSQLQRDLLLMESIAIPLSFVVLVWVFGGLLAAALPVAVGLMAI
                     LGSMAVLRLITFGTDVSNLAPNLSTALGLALAIDYTLLIISRYRDELDEGADPHAALI
                     RTMTSAGRTVLFSATTVALSMAALILFPMHVLKSFGYAGVATVAFAALAAVVVTPAAI
                     VLLGGRLNALDARRFARRLLHRPDPVHRPTEAQFWYRSTTFVMRRALPLGLAVVALLL
                     ILGAPFLGVKWGFPDDRVLPTSASAHQLGDQLRNDFADNSATGVTVMIPNADGLTSVD
                     LGHYAAELSSVPDVSAVSAPTGTYVAGAPIGPPSAPTGVSQGSAFLTVDSTAPLFSDA
                     SQTQLDRLHAVPGPAGRPVELTGIAQTNRDSVDAITSRLPAVLGLIAVITFGLLFLLT
                     GSVVLPLKALVLNVLSLTAAFGALVWIFQDGHLAALGTTSTGNLVANTPVLLFCIAFG
                     LSMDYEVFLVSRIREYWLRSGRTRADNDKSVALGLARTGRVITAAALVMSISFAALFG
                     AHVQFMRMLGLGLTLAVLVDATLVRMVLLPAFMHLLGRWNWWAPKPLIWLHDRIGLRE
                     GEELAERTTLPVAEPV"
     gene            11768..12388
                     /locus_tag="VFC01_28050"
     CDS             11768..12388
                     /locus_tag="VFC01_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN83506.1"
                     /translation="MTAANAARPRADGDKSNFRQRLLDALEASIAEDGYPRTTVAGIV
                     RRARTSRRTFYEHFESREACLVALLTDANADQVRQIAGAVDPHAPWRSQVRQAVEAWI
                     SSGESRSALMLSWIRDVPSLGVAARGLQRDAMENFIEMVQALGGTEEFRAAGIGPVSR
                     QRIIMLLGGLRELTAITVEEGGRVGDITEEAVDASIALLAPRVEPD"
BASE COUNT         2266 a         4058 c         3953 g         2152 t
ORIGIN      
        1 acaccacgca aaccaggaga tatgaatagt gaccctcgtc aacacgcaat gccgcctggc
       61 cgcgcggccg gtcggcctac ccaaggcaac cgactgacgt tcaccgagga gccggtgccg
      121 agtccggccg aaggtggact cctcgtgcgg gtggagtacc tgtccatcga tccggcgatg
      181 cgtacctgga tgaatgctgg gcgaactggc tgcgcagcgg caagctggtt ctcgcgctct
      241 catcctgagc aggcaggacg cgggccgatt ccgactttgg tcgagggacg gcagcagatg
      301 tgtctcgcag gatctagcac cggtgatggc ccgcctggtg gccgaaagat tatgatgtgt
      361 aacccatttc gcacagtgcg agccgcagtg ctcgtaatgc gtagtgcagc ctcgacttca
      421 cggttccatc agcaatcccg aggtcggcgg cgatctgcgc cgtcgtccga ccttgaaggt
      481 acgagcgctg aaccacggcg cggtggtccg gggtcagccg cgccagcgct tcgccgatca
      541 gcattcggtc cagcgcggca ttcacctcgt ctgggcaggt gcgctcaggt gcacccgaga
      601 tgtccagcgc gctcacctca cgacggaacc gtaggctgcg actttcgtcg ataatcatgt
      661 ttcgggcgac ggtgaatagc cacgaccgtg ccgaccgctc ggtgtcgttg gcaacctcgg
      721 ggtgctgcca tgcgcgcagc agcgtctcct gcacgacatc ctgagcgcgg gccgggtcac
      781 cggtcaaccg acaggcgtaa cgccacaaag cggcggcgtg ctcgtcgtag agccccttga
      841 cggtggcggc ctcgcgaccc tctacggtct tcaccgctgc gcggtcgttc cggggcttga
      901 gctcagaccc caacatgcgg tcgctgcagc cggcgaaatc ggctgcagta atgtcagttt
      961 cggtgctcgt cgtcatgttt tctactcatt ccgatgcgta gctacgccgt cctgtcagct
     1021 aaaccggctg cgacagtgca gcatcccgcc ggacggatag gccgcgttcg tcgactacac
     1081 gttccgcggc gagagccgcg gggtaattgg ctaggcagcc ggaaggtggc gattcctaac
     1141 accgacaatg gcattcgcga ggacccaagt gcaggtagcg ccaacgctag tagtggcgct
     1201 actagtctct atctcggcgc atcccgagca ccttgcacat ggcgttgatc aggccaaaga
     1261 gctccaaata gctccgagag ccaggcaccg attccagcta gctggaatgc aatgttgggt
     1321 gccagccggg gcgacaagtt ctggcagacg ggcaatcgtg ggcatagcga cgggaaacta
     1381 gcaattgggt tcccaaacag atctgacctc ggagcgagga cacatgacac cgagttcgcc
     1441 cgagttcggc acacccacga gtctcggcat ggcagctgac accgcgtcct accgaaatcg
     1501 ctgaggcgca acattgaagc aaaacgcggc catgggccag ttccttacaa gccgccggca
     1561 tcagctcgta cgcgccgacc tcggcctgcc cccgatcgcc ggccggatga ctcgcgggct
     1621 gcgtcgagag gaagtggcct acctggccgg tgtcagtgtc acctggtaca cgtggctcga
     1681 acaggcacgc gacgtcaccc cgtcccggca agtcgtcgat tcgctcgctc ggaccctgcg
     1741 gttgtcgcac gcggaacacg tccatttctt ggcgctggcc gggtacagcg cgcctcctcc
     1801 cgccgaagac ccgagtcccg gcaccgcccc tgctcacgtg cagcggctgc tcgacgccct
     1861 cgcggactac ccggcgttag cgatcgcccc cgactgggcg atattggcat ggaatgccgc
     1921 atacgcgggt ctgtacccga acgtggcaac agtcgccgcg gccgaccgca acttcctatg
     1981 gctgcttttc accgacccct acctgcgcgc gctgatgccg gactgggaat tcaccggcat
     2041 gtataacgtc gcgtcgtttc gggcacaggc cggtactcgg ctgggcgagc cgccgttcgc
     2101 cgacctcgtc agtcggcttc tccagacgag tgaagcgttt cgggccgcct gggaaagcta
     2161 cggcatcgaa acactgccct cacgccagcg gatattccgc caccccgaag tcggcgacct
     2221 tcacgtggaa caacacaacc tggcgccctc gggcgatccc ggtctgcagg tggtgatgtt
     2281 tacccccata ccgcctaccg acacggccga gcggctacgc cgcctgcatg atatgcaatc
     2341 cctacgtctg tctggtgcgt gaggtcgcca aagacggaca tctccgaaac cgattcagcc
     2401 cgtattcatt ctgtgcgtgc ccaagtcgta cggctgcggc agcggtcgcg ccctgaccgg
     2461 atgaaccaca aacggttctg catcgtgtta ctggcggggc accgtgacga tggagtcacg
     2521 ccgcttatgt tcagtaatca ccgcggggag taactctgtc aacatcgtct gcagtcacgc
     2581 tgaccgccgc tccaccgctg gtgctgctgc atcctttggg ttcgacggga gcgatgtcgt
     2641 gggcgtcact tgcggattcg cttcgggcag tgggctatcg cgtagcggca ccggacctgc
     2701 ccgggcacgg gacctcccgc gagacctcgt ttacgtggga gagtgctagg gcgacggtcc
     2761 gtgccgcagc cgagcagttt gggccggaca agccagtcct tatcggtctt tcgcttgggg
     2821 cggcgaccgc gatatacgcc gcccagcgtg agcccgccag cttcgccggt cttgttctcg
     2881 ccggagctgg ggcttgctgg aacgaccggt gcctcaggac agggttgacc gcggctgcag
     2941 ccgtcggagc gctattggca gcggttggcc gccgcgaacc gctcgcccac gccgcgggtt
     3001 tccggggccg cgagatcttc attgccgccc gtactgccga tgtcgcaccg gcccagctgt
     3061 ggcgcgccgc aaggcaattg acgcgcttcg acttgcgtcg cacacctccg ccaccaatac
     3121 cgtgcgccgt catcgtgctc accgatgacc ggcggatgcc gcccggcttg cagcgggcac
     3181 tcgccaggta cttggactgt ccgcatgtcg atgtggccgc tgaccatgac gcaccggtct
     3241 gccagaccgg ccgattctcc gacggcgtgc acgctgcgct cgtttcactt agaccgcacg
     3301 gaagaggaat tccactatga ccacacaggt cgatgacagc ctcgacgctg ttgtcatcgg
     3361 tgcgggccca gcaggtctcg ctgttgcacg tgagctcgaa catcagcacg gcatcgagac
     3421 gctcgttgtc gaccgtgcag cggcgcccgc catttcgtgg cgcacgcgct atgacaactt
     3481 ccggctcaac acaaccggct tcttgtcgca tcttcctgga cagcggatcc cgttgaccgc
     3541 cggccgttgg ccaacgcggg aggacatggt tcgctatttc gacgactacg tgtcgaggca
     3601 gaacatcacg ctcgaactgg gctgtgaggt caacgggata gatcgtgctg gaggcggctg
     3661 gcggctcgac acctcgtcgg gcgaaatccg aacgcgggca atcgtcctcg ccactggcaa
     3721 ctaccgcacg cccaccatcc cttgctggcc ggggttgagc gcattcaccg gcgaattcgt
     3781 ccactccggc agtttcaaga acgcttggcc cttccgcgac cgcgacattc tcgttgtcgg
     3841 cggaggcaat tcggccgctg acatcgccgt ccagctcgcc aacaacggcg cgcgccggat
     3901 ctggctttcg gtccgcacac cgccacatct cgtgcggcgc gccatcgcag ggtttccgtc
     3961 ggacatcttc ctcgagctct tcgcctgggc gcctgcgagc gctgtcgacc cgatcatcgg
     4021 gctctccgag aacctgatgt ggggcgacct gtcggcctac ggcttcaacc ggccgccgct
     4081 cgggctaaag gcgacggtag aacagacagg ccgcattccc acactggccg atgagcttat
     4141 cgacgccgta cgagccggtc gggtggaggt cgtcgccgct gtggaggcga tcgaatccgg
     4201 ccgtgtgatc ctcgccgacg gcaactccat accgccgggt gtgatcatcg ccgccaccgg
     4261 cttcagcatg gatctcgacg gtttggtcgg ccacctcggc gtgctcgacg aacgcggaaa
     4321 accacgcggc ggattcgcct cgcacatcgg tgacggaatg ttcgcgatcg gctacggcat
     4381 tccgcccaat ggcccactgc gcgcgatccg cctcgccgcc acgcctctgg cccgtgagat
     4441 cgcagcccat ctagcggcga ctcacgcgtc acagcaggtc acatctcaca gctgacttcc
     4501 agccacggcg atagccgaaa tcgcggcgat ataagtacgt ttcgatcgat ccgcgatcag
     4561 ctattgactg ggagtctgtg acatcgagca ctctcgatga acagaataca gtcgcacgtc
     4621 gtgtcactaa cagccgcggg ttgcggccga ggtgcgcagc gaagagtgcc gatgttcatc
     4681 cgaacggcgc tgacaccgct atgcgaaaat caaggggacg taatgggttt ggtttccgtc
     4741 gacaagtact acttccgcgg cgcggaagga tacgagacgg cccgtcgggc gacggtgtgg
     4801 aatggccttc tgccggaccg ctttcccgac gtgatcgtgc aagcccacga caccgacgac
     4861 gtggtcgcgg cgatccgcta tgcgcgcgcc aacggccata aagtcggcat acgttcgggg
     4921 gggcacagct gggcggccag tcacctgcgc gacggcggtt tgctgctcga cgtcagccgc
     4981 ctcgaccact gcaccgtcga cacagatcgg atgaccgcgg acgtagggcc gggcaagatc
     5041 gccaacgtct ttgcggccga gctcgactcg caagggctgt tctttcctac gggccattgc
     5101 gaaggtattc gcctcggggg ctacttgttg caaggtgggt acggctggca cagcaaggtg
     5161 ctcggaccgg catgtgaaaa cgtgcttggc cttgaggttg tcactgccga cggcgaccag
     5221 atctactgcg acccccagaa ccatcccgac ctgtactggg ccgcccgcgg ctcgggccct
     5281 ggcttcttcg gtgtagtcac ctcattcaaa ctgcggattc acccgcgccc gtccgtcttg
     5341 ggcacctgcc tgtacatgta cccgatcgag ctcgccgacg aggtgttcac ctgggggcgc
     5401 tccatcagtc ccgagatcga cgcccgcgtc gaactgcaaa tcctcaccac acggtcattt
     5461 ccggcagccg ggatcgacca ccccgtcatc accatcgcct cgccagtctt cgccgaaacc
     5521 gaagaggaag cggcgaaagc acttgccgca ctgggcacct gcccggtcgt cgatcaggcg
     5581 atgatcaatg tcccctatgc gccgacaaac ctggccagct ggtacaccgc ggtgatgacc
     5641 aactacccga aagggcaccg gtatatcgcc gacaacatgt ggacgtcggc ttcagctgaa
     5701 gaactactgc cgggcatccg caaaatcatc gaaacgatgc caccacaccc ctcgcacttc
     5761 atattcacgg gctggaacgc gtccccggac cgcccggaca tggcgttcag cgtggaggac
     5821 gagatctata tggcgctgta caccgtctgg caggatccgg ccgacgacga acggtaccgc
     5881 gactggtcag ggtccaacat ggcggcgatg tcgcacctgg cgaccggcac cgcgctcgcc
     5941 gacgagaatc tcggccgacg cccggcgaaa ttcatcaccg acccgaacat ggcgcgcctc
     6001 gacaaggtgc gctcgaccta tgatccggac ggtcgcttcc acagctggat gggccgggca
     6061 tgaaaatggc tgtaccgaac gcactccgga tcctcgtcat cggcgccggc gtgggcggca
     6121 tttccattgc tcggggacta ctgcgagacg gacatgacgt caccgtcttc gaacagcggc
     6181 cggatatgcg gcccggtggc ggtgcggtga ccatctggcc caacggctca acggttctgg
     6241 agcagctggg cgtcgatatg cacggggcag gtcagctgct gtccaccgtg cggatcgcga
     6301 catcgactgg tcgcccactg gtcaacatcg acgcgaccgc gatcgtgcac cgactgggcg
     6361 gttcggttcg gatggtcccg cgtcgagcgt tgcttgaccg tctgctggaa ggctttccgg
     6421 tcgatcacat ccgatgcaac tcccgtgttg tcggggcggt cggcacgcgc gatggggtgc
     6481 ggatcgagtt cgaagacggc agctcggccg aaggcgatct gctgatcggg gcggatggcc
     6541 tgcattcgat ggtccgagat atcgtcggcg cgaagcccgc gaaggcgacc ggctggtgca
     6601 gctggcaggg actgatcgcc cttccggaca tcgccgacaa ggacgtcgca ctgcaagtca
     6661 tcggcgagca cggaaacctt ggcctctggc ccgccggtgg ttgcgatctg cagtggtggt
     6721 tcgacttgcg gtggtcgccc gacttcgtca gaccggaacg tccgatcgag atgatccgcg
     6781 ctaatttcgc aggctggtcc gacttggtcg atcaagtact cgcgacgttg accgaagacg
     6841 atctggcggc ttcgccgttc ccgcattttc ggcatccgat tccccgctcg ccgcgcctgg
     6901 gtgcgatgac actgctcggc gatgccgcgc acacgatgcc gccgatcctc gcgcagggga
     6961 ccaatcaggc gctgctcgac acgatggtgt tgcgcaaggc actttcggat ttccggaagg
     7021 gacccaactg cggcaacagt gatctcgcga gcgcactgcg gtggtacgag aagaccagga
     7081 gacgcaggct caccgccgtg tcctgggtga cgtcgcggca gatgtcccag agcgagtcgg
     7141 tgttgaaacc ggccgccatg atctcggact catttggcac ctgggcgatg acgacgtttc
     7201 tgcgttcgat aagccaccgg cggatatccg cggaagtcaa ccgagacctg tctagccggc
     7261 tgatccgcac ctgatgatca tccctacgac tgtgaaggat cgggcgatgt cgaaaaccgc
     7321 caaggtgcca ccagcgaagc tggcccgcgt catcgagtgg actcgccacc acctgtatcg
     7381 gctccatcaa cggctgacac ccgcgccagc ggcaatgatc gagctgatcc ttgccggctg
     7441 gacgtcgcag gcgatcaccg tggcggccca actcggcgtc gccgatgccc tcgccgacgg
     7501 tccgttgacg atcgacgaat tggcgacgcg agtggacgcg aacgcagacg cactgcaccg
     7561 gttgctgcgg gcgttgatca gcaggggcgt cttccgtcat cgccgcgacg gccggtttga
     7621 gctgaattcc cttgccgaca ccctgcgttc cgatgcaccg gcgtcgatga aatcggccgc
     7681 acggttctac gggtcgcagg aacagcgcga acgctggacc cttctggtgg attcggtccg
     7741 aaccgggact tcggtcgtcc cggcattgcg cggcaagtcg agcttcgact acttcgccga
     7801 cgagcccgag cttgccacac ttttcaacca gaccatgacg agcgtttcgg aaatgacgga
     7861 tgtgcccgtg gttgccggtt acgacttcag cgcctacccc acgatcgtcg acgtcggcgg
     7921 cggccatggt cccctgctgg ccaccatcct ggcggcgacg ccggcttcgc ggggcgtact
     7981 gtatgacctg ccgtcggtcg tcgcgaacgc cccaaacctg ttgcgggagc acgatgtcga
     8041 aaaccgggtg cgtattgagg agggttcgtt tttcgacagc gttccggacg gtggtgacgc
     8101 ctacgtcctc aagaacatca tccacgactg gcgcgacgag aaggcgctgc agatcctgcg
     8161 taatgttcgc gcggcagccg gcccccgagc cacggtgttg ctgatcgagt tcgtcatccc
     8221 caaccacgac cgtgacttcc cgggaaaatg ggttgatctg gagatgctgc tgaacttggg
     8281 ggcccgcgaa cgcaccgcgg ccgagtaccg agacttgctc agtcaggccg gattccgttt
     8341 gaccagggta gtgcaaaccg ctgcacccct cagcgtggtt gaggccagag ccgcatagcg
     8401 acaacgagcg gcacacagga gcgagaatca atcgagatga caagtcgacc ggtcagctga
     8461 taacgctcgg cttggtgccc ctcgtgttat gcggtggtgt gtgattcagc aacgcacgtc
     8521 aacatagacg gtgttggaga cgacctgccg agcgtggttg ccacgacccc ccgtgggagt
     8581 ttcctgtgtg atctgttggc cggggcggat cgcggcgacc gagcaattcg acgactcggc
     8641 cccgctgagt cgggtcacga tgaccttata gccttcggct tgcaggctcg cgacggtgtc
     8701 gtcgaccggt gagggaccag agggagcggc tgtggcggca cccggcattc ccagcgcggt
     8761 gaggaccagg gcgcctacgg tcatggtgat ggctaaatgc ttcatggtcg aacctctctc
     8821 gtcagtgcac tgccgtgccc ctcgcacgcc ctgccactgt attgatagaa caatgcaaga
     8881 aacgggggaa cttgcggagc caccactatg tataactccc aagggggaca aagtattccg
     8941 tcgcatagct tgcactactc cgcaattcag tacaccccaa aagtcccgcc gtttactagc
     9001 cggtcaccgc gtaaccccgg cgagcgcaac ggcgcccggc tatccgagaa gatcgggtca
     9061 ccgctgtcgt cgcgacggta gcggcgcatt ggacgcggcc gctggatcgg acggtggcta
     9121 ccgagccggt ggaacatgct gcggtcaaac aggttccgcg acaggcagcg tggtgcgctc
     9181 ggccaactct tcaccctcgc gcaggccgat gcggtcgtgc agccatatca acggtttcgg
     9241 tgcccaccag ttccatctac ccagcaggtg catgaaggcg ggcagcagca ccatccgcac
     9301 caacgtggcg tcgaccagta cggccagcgt caggccgagc ccgagcatcc gcatgaactg
     9361 cacgtgggca ccgaacaggg cggcgaacga tatcgacatc accagggccg cggcggtgat
     9421 cacccggccg gtgcgagcca gccccagtgc gacgctcttg tcgttgtcgg cacgggtccg
     9481 ccccgatctc agccagtatt cgcggatccg agacactagg aacacttcat aatccatcga
     9541 caggccaaat gcgatgcaga acaacaacac cggcgtgttg gcgaccaagt ttccggtgga
     9601 cgtcgttccg agcgcagcca ggtgaccgtc ctggaagatc caaaccagag caccgaacgc
     9661 ggccgtcaac gacagcacat tgagcaccag cgccttgagc ggcagcacga cgctgccggt
     9721 cagaaggaac aacagtccga acgtgatcac cgcgatcagc cccagcaccg cgggcaatcg
     9781 tgacgtgatc gcgtctacgc tgtcgcggtt ggtctgcgcg atgccggtca actcgaccgg
     9841 ccggccggcc ggtccaggta ccgcatgcag ccggtccagt tgggtctgcg aggcgtcgga
     9901 aaacagtggg gccgtgctat cgacagtgag gaacgcgctg ccctgcgaca ccccggtggg
     9961 cgccgaaggt ggcccgatcg gcgcaccggc gacgtacgtg cccgtcggtg ctgaaaccgc
    10021 cgaaacatcc gggacgctgg acaactcagc ggcgtagtgc cctagatcga cagaagtgag
    10081 gccgtcggcg ttggggatca tcacggtgac cccggtggcc gagttgtcgg cgaagtcatt
    10141 gcgcaactgg tcgccgagct gatgcgccga cgccgacgtc ggcagcaccc ggtcatcggg
    10201 gaagccccat ttcaccccga ggaaaggcgc gccgagtatc agcagcagcg cgacgacggc
    10261 aaggcccagc ggaagggcgc gacgcatgac gaacgtcgtt gaccgatacc agaactgcgc
    10321 ttcggtcggt cggtgcacgg gatcgggccg atgcagcagg cgccgcgcga atcgccgggc
    10381 atccagggcg ttcagccgac cacccagcag cacaatcgcc gccggcgtga cgaccaccgc
    10441 tgcgagcgcg gcgaacgcga cggtggcgac accggcatag ccgaacgact tcaggacgtg
    10501 catcgggaac aggatcagcg ccgccatcga caacgcgact gtcgtcgccg agaaaagcac
    10561 cgtgcgccca gccgatgtca tcgtgcggat cagggcggcg tgcgggtcgg cgccctcgtc
    10621 cagttcgtcg cgatagcggc tgatgatcag caacgtgtag tcgatcgcga gggcgagacc
    10681 gagcgcggtg ctgaggttgg gtgcgaggtt cgacacatca gtgccgaagg tgatgaggcg
    10741 caggaccgcc atcgagccca ggatcgccat cagcccaacc gcaaccggga gtgcggccgc
    10801 cagcaggccg ccgaacaccc acaccagcac gacgaagctc aatggaatgg cgattgactc
    10861 catcagcagc aggtcgcgct gcaactggga gttgatctgc gcgttcacca tggcgtcccc
    10921 gcccgatcgg acgatgacgc cgttcgtccc gaccggatga tcgcgtgcga tctcatcgga
    10981 gagcgtctgg gccgttgtct gcgcgacact ctctccgccg ccgatgcccg cgacgacaag
    11041 tccggccttg ccgtccttgc tgactaggcg ctccgccgcc gccggtggcg cagtccacgc
    11101 cgaggccacc gcagtgacgt tcggcgaccg cgccagctga tcgacgatgg ctaaacccac
    11161 cgcacgggcg gaccggccat tggcgctgtc cggagcggtg actgtgatga gcatctgcat
    11221 atcgccctgg tggaacttgt cggtcagcag ctgtgtggcc ctggctgact ccgaggtcgg
    11281 gtcctggaat ccgctcgagg acaggcgttt ggcgaccggt ataccgaaga tgcccgccgc
    11341 cagcatcagc aacagaatga ccgcgatgac ccgccgcggg gctgcgatcg ccagcaatgc
    11401 gattcgttgc agcacgtcgt cctctccgta cccgcggacg cacgatcagc ggcaacttat
    11461 tcaccgtaag tcaacaagta ctacgaaatt cggccccgcc gggttcagcc gtcgacgccc
    11521 gcgcgctgga aacggccata tatttcaact ctctcaggca cgcgatgtcc gcagtgacat
    11581 cgacgaagaa acggggaatg ttctgccgca catcgacggc gaccccgccg cgcgtgacga
    11641 tgagtccgca tggcgagagc gttgttcacg gcagccgaag cgcgcccgtg acgtgtgccg
    11701 cccaggcgcc atcggctcgg tgagcggtgt cgtcgcagta cgatgcctgt gacatgagag
    11761 gtccccagtg acggcagcca acgcggcgcg accacgcgcc gacggggaca agagcaactt
    11821 ccgccagcgc ctccttgatg ccctcgaagc gtcaatcgcc gaagacggct acccgcgtac
    11881 taccgtcgcc ggcatcgtgc ggcgggcgcg gacctcgcgg cgcactttct acgaacactt
    11941 cgagagcaga gaagcctgct tggtcgccct cctgaccgac gccaacgccg accaggtccg
    12001 gcagatagcc ggcgctgtcg acccgcacgc gccgtggcga agccaggtgc ggcaggcggt
    12061 cgaggcctgg atctcgtcgg gcgaatcgcg ctccgcgctg atgttgagtt ggatccgcga
    12121 cgtgccgtcc ttgggcgtcg ccgcgcgcgg gctgcagcgc gacgcgatgg agaacttcat
    12181 cgagatggtt caggcgttgg gcggcaccga ggagttccgt gccgcgggca tcgggccggt
    12241 ttcgcgtcaa cgcatcatca tgctgctcgg cggcctgcgt gaactcaccg cgatcaccgt
    12301 cgaggagggc ggccgggtgg gcgacatcac cgaggaagcg gtggatgcgt cgatcgcgct
    12361 gctggctccg cgcgttgagc ccgattagtc ggcgacggtg atcgcctgcg tcgggcacga
    12421 gtcggcggc
//
LOCUS       DASRJA010000623         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3743399,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000623 DASRJA010000000
VERSION     DASRJA010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3743399"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFC01_28055"
     CDS             <1..618
                     /locus_tag="VFC01_28055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83507.1"
                     /translation="LAIDPEHQAALHNLAVIDLARGRVDAAARKFTDALSFAPHDAIT
                     GQAHAESARGVLWRLFDVMALAVAAHVLLFNAIDRPLGAWRRPVELAAAALILAGFGW
                     LAARKWTAQPRGIRWRLRADIRKAVVVFGVILIVMTAAGLILSAYAPAARSVLDDLRA
                     WFLFVPFAVLVVRLRNMLLRQIYPTTRRLWFRLWTARGRRRAPDA"
     gene            1005..1919
                     /locus_tag="VFC01_28060"
     CDS             1005..1919
                     /locus_tag="VFC01_28060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83508.1"
                     /translation="MSEMSRRTVLAAGGAVLTAFLAGCARSTSGGDAGIGGQSNTDPF
                     GVDPAVLDGWRNFPVTRTPRPILLLGAPLRETGYHTDDAKLAVATGRYELAATLPSTN
                     PTTSPATLPDGTFQLPIITAAQAYERLRATGDPANAPGESPAPLQITRVELGTASFQT
                     DRGALDLPAWLFHAPDSFEPLAWPAPHPDAFWRLGDLPVGGDVSDGRLAADAVTLTVT
                     MPAPHPGACPGDPIYRHDPVVLEESTVVAVGVRREIVSIAPGSRRDDCAYDLMLRVQQ
                     YTVTLAAPLGNRVLVSSAGMPAAVTVSG"
     gene            2231..3379
                     /locus_tag="VFC01_28065"
     CDS             2231..3379
                     /locus_tag="VFC01_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HZN83509.1"
                     /translation="MTAHLTMEQRQLARRLSAKGLSLREIGRQVGCSHEIVRSVVRGE
                     SKRPVRSDGWQPGPGRLTLADREEVSLGLRSGDSFSVIAIKLGKAVSTVSREVGGNGG
                     RDDYRAWRAHQRARDRARRPKTPKLSCPRLAGQVTLWLQEWWSPQQIAGRLRLDFADD
                     PMMHVSHETIYQALFVQGRGELRRELVRCLRTGRVRRRARRRPENTGQLRDMVMISER
                     PAEADDRAVPGHWEGDLLIGGDCRSAVGTLVERTSRCVLLLHLPDGRSAKGVERAMQQ
                     AISTLPEQLRRSITWDQGKEMAHHAQFTISTGIQVYFCDPHKPWQRGSNENTNGLLRQ
                     FMPKGTDLSVHTADDLARYEHSLNNRPRKTLGYMTPLERLAELLAHTR"
BASE COUNT          587 a         1176 c         1168 g          581 t
ORIGIN      
        1 ctcgcgatcg atccagagca tcaggccgct ctgcacaacc ttgccgtcat cgaccttgcc
       61 cgcggccggg tcgacgccgc cgcacggaag ttcaccgacg cgctctcgtt cgcgccgcac
      121 gacgccatca ctggccaggc gcacgcggag agcgcgcggg gtgtgctgtg gcgcctcttc
      181 gacgttatgg ccctggccgt cgcggcgcac gtgctgctat tcaacgcgat cgaccgccct
      241 ctcggcgcct ggcggcggcc ggttgaactc gcagccgccg cgctgatcct ggctggcttc
      301 ggctggctag cagcgcggaa gtggacggcg cagccgcgcg gcattcggtg gcggctgcgt
      361 gccgacatcc gcaaggccgt cgtcgtgttc ggcgtgatcc tcatcgtgat gaccgctgca
      421 ggactgatcc tcagcgcgta cgccccggca gcgcgctccg tcctggacga cctgcgggca
      481 tggttcctgt tcgtcccgtt cgccgtactg gtcgtgcgcc tacgaaacat gctgctccgc
      541 cagatctacc cgaccacccg gcggctctgg ttccggctat ggacggcgcg cggtcggcgc
      601 cgagcaccag acgcatgaca ccgtcggcgt ccggccgtca gcccttgacg gtgaattctt
      661 ataccacctc accgcggcgt tgttcggcga ggcgaggccg cgggcgccgg cgtgccattg
      721 cgaagaacca gtacatcctt atgagcgacg aggtgcgcgg gtaaagcatc acccggagtc
      781 agacacactg tccacttcgg tgcctggtct cacatgatgc atgacgaaac ggcctcgcag
      841 gatgcttaac atgatcacaa tgagtccggc ccgcgccatc atggaccgcg tcggacgcat
      901 ccacttatgt cgacatgagg ctcgccggcg ttcgacagcg gccgctacac gctaagttgt
      961 gcgcgtcaca tccgattagc cgagacatca acggaggcgc cgtgatgtcc gagatgtctc
     1021 gccgtacggt gctcgccgcc ggaggcgccg tattgaccgc gtttttggcc ggctgcgccc
     1081 gttcgacgag cggtggcgac gctggcatcg gaggccaatc gaacacagat ccgttcgggg
     1141 tcgacccggc cgtgcttgac gggtggcgga acttcccggt cacccgtacg ccgcggccga
     1201 ttctgcttct gggggcgccc ttgcgggaga ccggctacca caccgacgac gcgaagttgg
     1261 ctgtcgcgac cggacgctac gagctggccg cgacgctgcc gtcgactaat cccaccacca
     1321 gcccggcaac gctgcctgat ggcacgttcc agctcccaat catcaccgcg gcgcaggcgt
     1381 acgagcgcct ccgcgccacc ggcgaccccg ccaacgcccc aggtgaaagt ccggccccgt
     1441 tacagatcac cagagtcgag cttggaacgg cgtcgtttca gaccgaccgc ggagcgctcg
     1501 atctgcccgc ctggctgttc cacgcacctg actcgttcga gccgctggcc tggcccgcgc
     1561 cgcatccgga tgcgttctgg cgtctgggcg accttccggt cggcggggat gtcagcgatg
     1621 gtcggctcgc cgccgacgcc gtcacgctga ccgtgaccat gccggcaccg caccctggcg
     1681 cctgcccagg cgacccgata taccgccacg acccagtggt cctcgaggag agcaccgttg
     1741 tcgcggtcgg ggtgcgccgc gagatcgtct cgattgcgcc cggatcgcgc cgggacgact
     1801 gcgcatatga cctgatgctg cgcgtgcagc agtacacagt cacgctcgcc gcgccgctgg
     1861 ggaatcgcgt cctggtcagc agcgccggaa tgccggcggc ggtgaccgtc agcggatgag
     1921 gccgtgaacg aatcggacca ctgccgtcgt tggccacgat cctccacgcg gctggccgtc
     1981 gtagagggct tcgctctgcc atcgagagcc gccgctcatc gcgggctcaa ctcctactga
     2041 tccggggtgc gacacgacac agctcgcttg ttgcacgccc tagcgaaacg atcctcactt
     2101 gccgcaagtc gcgcggttgt tccgatcatg tcaagcatca gccgggacca cgacgggcgg
     2161 gttcaagggg tcaacgcacg atcttggtgg gtcagcgaga atctcgttga tctattgagg
     2221 gagatgtttg atgaccgccc atctgacgat ggagcaaagg cagttggccc gacggctaag
     2281 cgcgaagggc ctatcgctga gggagatcgg ccgccaggtt ggctgttccc atgagatcgt
     2341 gcggagcgtc gtgcgcggcg agtccaagcg cccggtgcgg tcggacgggt ggcagccggg
     2401 gccggggcgg ctgaccttgg cggaccggga ggaggtcagt ctcggcctgc gcagtggcga
     2461 ctcgttcagc gtcatagcga tcaagcttgg caaggcggtg tccactgtgt cccgggaggt
     2521 cggcggcaat ggcggccgag acgactaccg ggcctggcgc gcgcaccaac gggctcgaga
     2581 ccgggctcgt cgtcccaaga cgccgaagct gagctgtccc aggctcgccg gtcaggtcac
     2641 gctgtggctg caagagtggt ggtcgccgca gcagatcgcc ggtcggttgc ggttggactt
     2701 cgccgacgat ccgatgatgc atgtgagtca cgagacgatc taccaggcgc tgttcgtgca
     2761 gggccgcggc gagctgcgtc gcgagttggt gcggtgccta cgcaccggcc gggtcaggcg
     2821 gcgtgcccgg cgccgcccag agaacacggg ccagctgcgc gacatggtca tgatcagcga
     2881 acggccggcg gaggcggatg accgggcagt gccgggtcat tgggagggtg accttctcat
     2941 cggcggagat tgccgctcgg cggtcgggac cctggtcgag cgaacgagcc gctgcgtgct
     3001 gctgctgcac ctacccgacg gacgcagcgc aaaaggcgtc gagcgcgcga tgcagcaggc
     3061 aatcagcacc ctgcccgaac agctgcgccg cagcatcacc tgggatcagg gcaaggagat
     3121 ggcccaccac gcccaattca ccatctccac cggcatccag gtctacttct gcgacccaca
     3181 caagccatgg caacgcggct cgaacgagaa caccaatgga ctgctgcgcc aattcatgcc
     3241 caagggcacc gacctatccg tgcacaccgc cgacgaccta gctaggtacg agcacagcct
     3301 caacaaccgt ccccgcaaga cgctcgggta catgacacca ttggaacggc tcgccgagct
     3361 tcttgcgcac acccgttgaa cccgcccgaa acggcctcgc tggatacttg acgcggatct
     3421 agatcgatgt cggatcgaga ttagagcagc aggaggcagg ttcggtgagg gggttggcga
     3481 ttcgaggtct gtagcggcta agtgttcgcg gc
//
LOCUS       DASRJA010000624         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2022008,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000624 DASRJA010000000
VERSION     DASRJA010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022008"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFC01_28070"
     CDS             <1..399
                     /locus_tag="VFC01_28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN83510.1"
                     /translation="RRVHDRMDREYAQPLDVEALARDVNMSAGHLSRQFKLAYGESPY
                     SYLMTRRIERAMAMLRRGDMSVTEVCFAVGCSSLGTFSTRFTELVGMPPSAYREQTAH
                     VTEGMPSCVEKQITRPIRNREAPGTALHLA"
     gene            405..815
                     /locus_tag="VFC01_28075"
     CDS             405..815
                     /locus_tag="VFC01_28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN83511.1"
                     /translation="MDINIHSSMLPHEDPDQSLAFYRDTLGFEVRLDVGGGNMRWITV
                     GPPNQPDTNIVLYPPDATPGLTDDERRLIHEMMAKGTYAQINLATKDLDGTFERVQAG
                     DVEVVQEPTDQPYGVRDCALRDPAGNMVRIQEQR"
     gene            820..>2519
                     /locus_tag="VFC01_28080"
     CDS             820..>2519
                     /locus_tag="VFC01_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZN83512.1"
                     /translation="MSKSTRSPALHAADTHEVIRVHGARENNLKDIDVELPKRRLTVF
                     TGVSGSGKSYLVFDTIAAESQRLINETYSAFVQGFMPTLARPDVDVLDGLTTAIIVGQ
                     ERLGSDPRSTVGTATDANAMLRILFSRLGKPHIGSPQAFSFNVASISGAGAVTLERAG
                     KTVKERRSFSITGGMCARCEGRGSVNDIDLTALYDDSKSLNEGALTIPGYSMDGWYGR
                     IFRGCGFFDPDKPIRKYTKKELNDLLHKEPTKIKVDGVNLTYAGLIPTIQKSFLAKDV
                     DAIQPHIRAFVERAVTFTTCPECDGTRLSELARSSKIKKVNIADACAMQISDLAEWVK
                     GLDEPSVAPLLAALQHSLDSFVEIGLGYLSLDRPSGTLSGGEAQRVKMVRHLGSSLTD
                     ISYIFDEPTIGLHPHDIQRMNDLLLQLRDKGNTVLVVEHKPEVIAIADHVVDLGPGAG
                     AAGGEVVFEGTVDALRGSETVTGRHLDDRAELKESVRKPNGALEILRASTHNLQDVDV
                     DIPLGVLVVVTGVAGSGKSSLIHGSVAGLDGVVAIDQTAIRGSRRSNPATYTGLLEPI
                     R"
BASE COUNT          484 a          828 c          803 g          404 t
ORIGIN      
        1 cgccgtgttc atgaccgcat ggaccgcgag tatgcgcagc cgctcgatgt cgaggcgctc
       61 gcacgcgacg tgaacatgtc ggccggccac ctcagccgcc agttcaaact cgcgtacggc
      121 gagtcgccct actcgtatct gatgacgcgg cgcatcgaac gtgcgatggc gatgctccgc
      181 cgtggcgaca tgagcgtcac cgaggtgtgt tttgccgtcg gatgctcgtc cttggggacc
      241 ttcagcaccc ggttcactga gctggtcggg atgccgccca gcgcctaccg ggagcagacg
      301 gcccacgtga cggaaggtat gccgtcatgt gtggagaaac agatcaccag accgatcagg
      361 aatcgagaag cgccaggcac ggcgctccac ctagcgtgag caccatggac atcaacattc
      421 actcgagcat gctcccgcac gaagacccgg accaatccct cgccttctac cgcgacaccc
      481 tcggcttcga ggtccgcctc gacgtcggag gcggcaacat gcgctggatc acggtgggcc
      541 cgccaaacca gcctgacacc aacatcgtcc tgtacccgcc ggacgccacc cccggcctca
      601 ccgacgacga acgccgtctc atccacgaga tgatggccaa gggcacctac gcccagatca
      661 acctcgccac caaggacctc gacggcacct tcgagcgggt acaggccggc gacgtcgagg
      721 tcgtacagga gccgaccgac cagccgtacg gggtgcgcga ctgcgcgttg cgggatcccg
      781 ctggcaacat ggtccgcatc caggagcagc gctgacgcta tgagcaagtc cacacggtct
      841 cctgccctgc atgccgccga cacccacgaa gtgatccgcg tgcacggcgc gcgcgagaac
      901 aacctcaagg acatcgacgt tgagcttccg aagcgccggc tgacggtgtt caccggcgtc
      961 tcgggctcgg ggaagagcta cctggtgttc gacacgatcg cggcggaatc gcagcggctg
     1021 atcaacgaga cgtacagcgc cttcgtgcag ggcttcatgc caacgctggc gcggcccgac
     1081 gtcgacgtcc tggatgggtt gacgaccgcg atcatcgtcg gccaggagcg gctaggcagc
     1141 gacccgcgct ccacggtcgg taccgccacc gacgccaacg cgatgttgcg aatcctgttc
     1201 agtcggctcg ggaaaccgca catcggttcg ccgcaggcgt tctcattcaa cgtcgcctcg
     1261 ataagcggag caggtgcggt cacgctcgag cgagccggga agacggtgaa ggagcgccgc
     1321 agcttttcca tcaccggcgg catgtgcgcg cgctgcgagg gccgcggctc ggtcaatgac
     1381 atcgacctga ccgcgctgta cgacgacagc aagtcgctca acgagggtgc gctgacgatt
     1441 cctggctaca gcatggacgg ctggtacggc cggatcttcc gcggctgcgg cttcttcgac
     1501 ccggacaagc cgatccgtaa gtacacgaag aaggagttga acgacctcct ccacaaagag
     1561 ccgacgaaga tcaaggtcga tggcgtcaac ctgacatacg cggggctgat cccgacgatc
     1621 caaaagtcat tcctggccaa ggacgttgac gcgatccagc cgcacatccg cgcgttcgtg
     1681 gagcgggcgg tcacgttcac gacgtgtccc gagtgcgacg gcacgcggct gtccgagttg
     1741 gcccggtcgt cgaagatcaa aaaggtcaac atcgccgacg cctgcgcgat gcagatcagc
     1801 gacttggcgg aatgggtcaa gggtctggac gagccatccg tcgcgcccct gttggcggcg
     1861 ctacagcaca gccttgactc cttcgtcgag atcgggctcg gttatctgtc tctcgaccgt
     1921 ccgtcgggca cgctgtcggg cggtgaggcg cagcgcgtca agatggttcg ccacctcgga
     1981 tcgtcactga ccgacatcag ctacatcttc gatgagccga cgatcggtct gcatccccac
     2041 gacatccagc ggatgaacga cctgctgctg caactgcggg acaagggcaa cacggtgctc
     2101 gtcgtggagc acaagcccga ggtgatcgcg atcgcggatc acgtcgtcga cctcggcccc
     2161 ggcgcgggtg ccgccggcgg cgaagtggta ttcgagggca ccgtcgatgc gctgcggggc
     2221 agcgaaaccg tcaccgggcg acacctcgac gatcgggccg aactgaagga gtcggtgcgg
     2281 aaacccaacg gcgcgttgga aattcttcgt gccagtaccc ataatctgca ggacgtcgac
     2341 gtcgatatcc cgctcggtgt gctcgtcgtg gtgaccggag tggcgggctc gggcaagagc
     2401 tcgctgatcc acggatccgt tgcgggactg gacggcgtgg tggcgattga tcagacggcg
     2461 atccgcggct cccggcgcag caatcccgcg acgtacaccg ggctactcga acccattcg
//
LOCUS       DASRJA010000625         4238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3544727,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000625 DASRJA010000000
VERSION     DASRJA010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3544727"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFC01_28085"
     CDS             complement(<1..309)
                     /locus_tag="VFC01_28085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83513.1"
                     /translation="MAFLHQRRCFVITLVVALLAGCSPSDNTGSIEQASEPDDQVSPG
                     LIAFVSDREGSDALFLMRPDGSGVRRLTGELPPVSHPSWSADGQRIAFNAGSPIASDI
                     Y"
     gene            608..1504
                     /locus_tag="VFC01_28090"
     CDS             608..1504
                     /locus_tag="VFC01_28090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZN83514.1"
                     /translation="MRVVWRGTISFGLVTIPVRLYVATESKNIPSHQVHVSDGGRVQY
                     KRVCSIDGAEVPYEEIGRGYESATGEIVMLSEVDLAMLPAKTSRSIEVISFLPIQVVD
                     PIYLDRSYYVEPEPQGAKAYRLLRDALRKQGRVAIAKVTLRSREIVAALRVHGDVLVL
                     ATMLWPDEVREPSFPFQAQEDEWSTPGQELAMAEALIDSLSDEQLDPGRYRDVHRETL
                     TALIDAKATGRTPTQDIGPGAASVDLLAALRASIDAAKQRRSTADNGGRRRTATSTGA
                     AKKKPKKPAQAEREAHVPKKRG"
     gene            1586..1789
                     /locus_tag="VFC01_28095"
     CDS             1586..1789
                     /locus_tag="VFC01_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83515.1"
                     /translation="MSSCSDCGLDVDHCHGTLIMHDDRAVDCTDAACDLPDLFRHAFI
                     VDCIAVRGGCCGSDELIDWAEAS"
     gene            1849..2844
                     /locus_tag="VFC01_28100"
     CDS             1849..2844
                     /locus_tag="VFC01_28100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83516.1"
                     /translation="MTGRELDEIVRELYVLPPTNFVAARNELVRQARAAGSRDVAERL
                     QGLRRPTRSAWLVNLLARDPVAMQRLSALGRELRDAQTGLAGAELRSLAEQRRQLIAD
                     LLDRAQAHAAESGVRVTPAVLSEVEATLQAALVDLAGALTIRNGRLVRPLSHSGFGPR
                     PPLHVSEGRSTLELRPVEVPAETTAHAEGPPVEDELEARRQRRAVGDRDRWTVQIVEV
                     ADDWFVADDDVGSGSESGGPAGRELRLAEEVLAAAEAAHWQSEFDLADAEAAVEAADD
                     VVSSLDTKRIEARRDKVTAQRHLTQAQSRQRAAVNALAEARRRLDAARELAGRDE"
     gene            2954..3661
                     /locus_tag="VFC01_28105"
     CDS             2954..3661
                     /locus_tag="VFC01_28105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83517.1"
                     /translation="MDIVLIPGLWLDASSWDEVIPSLRQAGHRTHPLTLPGMESVGAD
                     RSKITLRDHVDAVVEVIDSLDANDGQLVLVGHSGGGAIAHAAVDARPDRITRVVYVDS
                     VPLGDGAVINNEFPTENGEMPLPDWSRFEEEDLVDLDDERRAKFRERAIPSPAHVATD
                     PQQLFDERRHDVPITVISCEFPSSEIQELLEQKHAYVRELAKVRDVVYVDLPTGHWPQ
                     FTRPEELGRAILASLAP"
     gene            3776..>4238
                     /locus_tag="VFC01_28110"
     CDS             3776..>4238
                     /locus_tag="VFC01_28110"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HZN83518.1"
                     /translation="MFENELVELDAAQTLAAAEANEHTLITAETRRLHLAAHWADLHP
                     GDAVVQSRLPGTEHPVQLGGDGTPTVGDFAPAELGCVLRISDGSACRLIADALDLRHR
                     LRTIWTAVQAGQVPAYQARRIASATRHLTVEQAAFVDARLAPSIGAVSWGRL"
BASE COUNT          764 a         1315 c         1426 g          733 t
ORIGIN      
        1 gtagatgtca ctcgcgatgg gcgatcccgc attgaaagcg atccgctgcc cgtccgccga
       61 ccaggaggga tgggacaccg gtggcagctc tccggtgagg cgccgtacgc ctgagccgtc
      121 gggccgcatc aggaacaagg catcggaacc ttcacggtcg gagacgaagg cgatgagccc
      181 tggcgagacc tggtcatctg gctcagacgc ctgctcgatg gaacccgtgt tgtcagacgg
      241 gctgcacccc gccagcaagg cgacgacaag ggtgatgacg aagcaacgac gctggtgaag
      301 gaacgccatg gatcgactat gggcgctttc tcccgagagt gcggcgagtg aggaggcctg
      361 aatcaattca aaagcgcaat tgtggcgtca ctcttcggtt gcccaaggag gcgtcagcca
      421 gctccgtacg ggactagatc gtcccaagaa tcgcgagtat gttcgagtgg tgccgacctt
      481 cggaacgaca gtcggcagac gttgtgccgg ggggtgaagt agatccgcgg gacccgcggt
      541 gacaatggag ggagccggta tgacggcggt attgacgggt caccaaccta gtcggaggga
      601 tacggcgatg cgggtcgtgt ggagaggcac catctctttc ggcttggtca cgatcccggt
      661 tcggttgtac gtggctactg agagtaagaa cattccctcc caccaggtcc acgtgagcga
      721 cggcggccga gtgcagtaca agcgggtgtg ttcgatcgac ggtgccgagg tgccgtacga
      781 ggagatcggg cgcggctatg agtccgcaac cggagagatc gttatgctca gcgaggtcga
      841 tctcgccatg ctgcccgcca agacctcgcg ctccatcgag gtgatctcat tcctgccgat
      901 acaggtcgtc gaccccattt atctcgaccg cagctactac gtcgagccgg aaccgcaggg
      961 tgctaaggcg taccggctgc tgcgggacgc gctgcgcaag cagggacgcg tagccattgc
     1021 gaaagtgaca ctccgcagtc gcgagattgt cgcggcgtta cgagttcacg gcgacgtgct
     1081 cgtcctggcc acgatgctgt ggcctgacga agtccgagag ccctccttcc cgttccaagc
     1141 ccaggaggac gagtggtcga cgcccggcca ggagcttgcg atggcggagg cgttgatcga
     1201 ctcgctgtcg gatgagcaat tggacccggg caggtatcgc gacgtccacc gggagacgct
     1261 gaccgctctg attgatgcca aggcgacggg gcgcacgccg acccaggaca ttgggcccgg
     1321 cgcggcatcg gtcgacctct tggcggcttt gcgcgccagc attgacgcgg ccaaacaacg
     1381 ccgatcaacg gcagacaatg gcggacgtcg ccgcaccgca acaagcaccg gcgcggccaa
     1441 gaagaagcca aagaagccag cacaggccga acgcgaagct catgtgccga agaagcgcgg
     1501 ctgacagcta actcgcgccc gcaactctgc gacacgcccg ttgcaatacc gcgtcctggc
     1561 tgatgcagat gatacgaacg acggcatgag cagctgttcg gactgtggcc tggacgtcga
     1621 tcactgccac ggcacactga tcatgcacga tgaccgtgca gtcgactgca ccgatgccgc
     1681 ttgcgacctc ccggatctgt ttcggcacgc gttcatcgtc gactgcatcg cggtacgggg
     1741 tggctgctgc gggtccgatg agctaatcga ctgggcggag gcgtcctaac cccggtttgg
     1801 gtcaaccggc aatcgccacg ttagattgac cggggaatga gtgtgcagat gaccgggcgc
     1861 gagttggacg agattgtccg cgagctctat gtgctcccac cgactaactt cgtcgctgcc
     1921 cggaacgagc tggtacgcca ggccagggcg gccggtagtc gcgacgtcgc agagcggctg
     1981 cagggcctgc gccgccccac acgcagcgcc tggctggtga atctcttggc ccgcgacccg
     2041 gttgcgatgc agcgcttgtc cgcccttggt cgtgagctgc gcgatgccca gaccgggttg
     2101 gccggtgcgg agttgcgcag cctcgccgag cagcgccgtc aactgatcgc cgatttgctg
     2161 gatcgtgccc aggcccacgc cgctgagtcg ggagtgcggg tgacccccgc tgtgctatcg
     2221 gaggtcgagg cgacgctgca ggcggctctt gtcgatcttg cgggtgcgct gacgatccgt
     2281 aatggccgtc tcgtgcgacc gctctcgcac agcggcttcg ggccgaggcc gcctctccat
     2341 gtctcggaag gcagatcaac tcttgaactg aggcctgttg aggtaccggc cgaaacaacg
     2401 gctcacgccg agggcccccc ggtcgaagat gagctggaag cccggcggca gcgtcgtgcc
     2461 gtgggcgacc gcgaccgctg gaccgtccag atcgtcgagg ttgctgacga ttggttcgta
     2521 gcggacgacg atgtgggtag cggatccgag agtggcgggc cggctggtcg tgagctgcgc
     2581 ctggcggagg aggtcctcgc tgccgccgag gctgcgcact ggcagtccga gttcgatctc
     2641 gcggacgccg aggcggccgt tgaggccgcc gacgacgtgg tgagctcact cgacaccaag
     2701 cgcatcgaag cccgtcggga caaggtcact gctcagaggc atctcaccca ggcgcagtcg
     2761 cggcaacgag cggcagtgaa cgcactggcg gaagctcgtc gtcggctcga cgccgcccgg
     2821 gaattggcgg gcagggacga gtagcgccag ccgaacacgt cagtgcccgc ggggttcagt
     2881 aggtggcgtg atcccggccc gttggcctgg ggagcttcgg ctgaccgcaa atggcttggc
     2941 tagcctctgg cgaatggaca tcgttttgat tcccggcttg tggctcgacg cctcatcgtg
     3001 ggacgaggtc atcccgtcct tgagacaagc cggacatcgc acacatccgc ttacattgcc
     3061 cggcatggag tcggtgggcg ccgaccgctc caagatcacg ctgcgtgacc acgtcgacgc
     3121 ggttgtcgag gtgatcgact ccttggacgc gaacgatggt cagctggtac tggtcgggca
     3181 ctcggggggt ggcgccatcg cccatgctgc ggtcgatgcg cggcctgacc gcatcacccg
     3241 tgtggtgtac gtcgacagcg ttccgcttgg tgacggtgcg gtgatcaaca acgagttccc
     3301 gaccgagaac ggcgaaatgc ccctgccgga ttggtcgcgc ttcgaggagg aagacctcgt
     3361 tgatcttgat gacgagcggc gtgcgaagtt ccgcgaacgt gccattccgt cgcctgcgca
     3421 tgttgcgacc gacccacagc aactcttcga tgagcgtcgc cacgacgtac cgatcactgt
     3481 catctcatgc gagttcccga gctcagagat ccaggagctg ctcgaacaga agcacgcgta
     3541 cgtacgcgag cttgcaaagg tccgcgacgt cgtgtacgtc gacctaccca ccggtcattg
     3601 gccgcagttc acccggcctg aggagctcgg acgggcgatc ctcgcgagtc ttgcaccctg
     3661 accctgaagc ggtccgggat cgggaagtgt tgtctgaatc ggtgaggtgt tgtccgttcg
     3721 gttcgacatg cccgatgcgc tgcggagtgt cggcgtctca ccatatattg aatgcgtgtt
     3781 cgaaaatgag ctggtggagc tggatgccgc gcagactctg gctgccgccg aagccaacga
     3841 acacaccctg atcaccgccg aaacccgccg cctccacctc gccgcccact gggcagacct
     3901 gcatcccggg gacgccgttg tgcagagtcg gctacccggc accgaacacc ccgtgcaact
     3961 cggcggggac ggcacaccca ctgtcggcga cttcgcaccc gccgaattgg gatgcgtact
     4021 gcgaatctcc gacgggtcgg cgtgccggct gatcgcggac gcgttggatc tgcggcaccg
     4081 actccgcacc atctggacag ccgtccaggc gggtcaggtg ccggcctatc aagcccgccg
     4141 catcgcctca gccacccggc atttgaccgt cgagcaggcc gcgttcgtgg atgcccggtt
     4201 ggcaccatcc ataggggcgg tgtcgtgggg acgactac
//
LOCUS       DASRJA010000626         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2657465,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000626 DASRJA010000000
VERSION     DASRJA010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2657465"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2777)
                     /locus_tag="VFC01_28115"
     CDS             complement(<1..>2777)
                     /locus_tag="VFC01_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HZN83519.1"
                     /translation="GQGIQSKGMGMEVRARSKAARKVWDTADKFTRDTLGFSVLHVVR
                     DNPTSLIASGVHYHHPDGVLFLTQFTQVAMATVAAAQVAEMREQGAFVEGAIACGHSV
                     GEYTALACVSGVYELEALLEVVFHRGSKMHDIVPRDHLGRSNYRLAAIRPSQIDLDDA
                     DVKAFVADISERTGEFLEIVNFNLRGSQYAIAGTVRGLEALEEEVERRREISGGKRSF
                     ILVPGIDVPFHSSVLRVGVADFRRSLERVMPRDKDPDLIIGKYIPNLVPRLFSLDRDF
                     IQEIRDLVPAEPLDEILADYDTWINEKPAELCRKVVIELLAWQFASPVRWIETQDLLF
                     IEEAAGGLGVERFVEIGVKSAPTVAGLATNTLKLPEYSHNTTEVLNSERDAAVLFATD
                     TDPEPDHEEEEAPAPVAESAPVEAAAPAAPAPAPAAPSGGPRPDDLGYDASDATMALI
                     GLSAKMRIDQIEPLDSIESITDGASSRRNQLLVDLGSELNLGAIDGAAEADLAGLKGQ
                     VTKLARTYKPFGPVLSDAINDQLRTVLGPSGKRPAYIAERVKKTWELGDGWVKHVTVE
                     VALGTREGSSVRGGELGGLHDGALADAATVDKVIDAAVLAVGARQGVSVSLPSAAGGA
                     GGVVDSAALGEFADQVTGRNGVLASAARLILGQLGLDTPVPVPEATDAELIDLVTAEL
                     GSDWPRLVAPTFDGRKAVLFDDRWASAREDLAKLWVLDETEIDTDWPRLSERFEGAGN
                     VVGTQATWWQGKALAAGRNVHASLYGRAAAGAENPGKGRYTDEIAVVTGASKGSIAAS
                     VVAQLLDGGATVIATTSKLDDDRLAFYRNLYRDNARFEAKLWVVPANMASYTDIDALV
                     SWVGNEQTESLGPQSIHLKDALTPTLLFPFAAPRVAGDLSDAGSRAEMEMKVLLWAVQ
                     RLIGGLSHIGA"
BASE COUNT          462 a          963 c          914 g          438 t
ORIGIN      
        1 tccgcgccga tgtgcgacag cccgccgatg agccgttgga cggcccacag cagcaccttc
       61 atctccatct cggcgcgcga cccggcgtcg gacagatcgc cggctacccg tggcgccgcg
      121 aacgggaaca gcagcgtcgg cgtcagggcg tccttgaggt gaatcgattg cggcccaagg
      181 ctttcggttt gctcgttgcc gacccatgac accagcgcgt cgatgtcggt gtaggacgcc
      241 atgttggcgg gcaccaccca cagcttcgcc tcgaagcgag cgttgtcgcg atacaggttg
      301 cggtagaacg ccaaccggtc gtcgtcgagc ttcgacgtcg tcgcgatcac agtcgcaccg
      361 ccgtcgagca attgggccac caccgacgcg gcgatggatc ccttggacgc gccggtgaca
      421 accgcgatct cgtcggtgta gcgacccttg cccgggttct ccgcaccagc cgcggcacgg
      481 ccgtagagcg acgcgtggac gttgcgaccc gcggccaggg ccttgccctg ccaccaggtg
      541 gcctgcgtgc caacgacatt cccggcacct tcgaagcgct cggacagtcg cggccagtcc
      601 gtgtcgatct cggtctcgtc gagcacccac agcttggcca gatcctcgcg ggcgctggcc
      661 cagcggtcgt cgaacagcac cgccttgcgg ccgtcgaatg tcggggcaac caaacgcggc
      721 cagtccgaac cgagttcggc ggtgaccagg tcgatgagct ccgcatcggt ggcctcgggc
      781 accgggacgg gagtgtcaag gccgagttgc ccgaggatca gccgagccgc cgaggccagc
      841 acgccattgc ggccggtgac ctggtcggcg aactcaccca gcgcggcgga gtccaccacg
      901 ccgccagcac caccggcagc cgacggcagc gacaccgaaa caccttggcg cgcaccgacc
      961 gcgagaaccg ccgcgtcaat gaccttgtcg acggtcgccg cgtcggcgag tgcaccgtcg
     1021 tgcagaccac cgagctcgcc gccgcgaacg ctggagccct cgcgggtacc cagcgccacc
     1081 tcgacggtca catgcttgac ccagccgtcc ccgagctccc aggtcttctt gacccgttcg
     1141 gcgatgtagg ccggacgctt gccggacggc ccgagcacag tgcgcagctg atcgttgatc
     1201 gcgtcggaaa gcacagggcc gaacggctta taggtccggg ccagcttggt gacctgtccc
     1261 ttcagcccgg ccagatcggc ctccgccgcg ccgtcgatgg caccgaggtt gagctccgag
     1321 cccaggtcca ccagcagctg gttgcgtcgc gacgacgcac cgtcggtgat cgactcgatc
     1381 gaatccagcg gctcgatctg gtcgatgcgc atcttcgccg acagcccgat caacgccatc
     1441 gtggcatcgg acgcgtcgta gccgagatcg tccgggcgcg ggccgccgga aggagcggcg
     1501 ggcgcaggcg caggcgctgc aggtgcagca gcctcgacgg gcgctgactc ggcgactggc
     1561 gcaggcgctt cctcttcctc gtgatccggc tcaggatcgg tgtcggtcgc gaacagcacc
     1621 gccgcgtcgc gctccgagtt cagcacttcc gttgtgttgt gcgaatactc gggcagcttg
     1681 agggtgttgg tggccaggcc ggcgacggta ggagccgatt tgacgccgat ctcgacgaac
     1741 cgctcgacgc cgaggccgcc cgcggcctcc tcgatgaaca gcaggtcctg cgtctcgatc
     1801 caccgcaccg ggctcgcgaa ctgccaagcg agcagctcga tgacgacctt gcggcacagc
     1861 tcggcaggct tctcgttgat ccacgtgtcg taatcggcga ggatctcgtc gagcggctcg
     1921 gcgggcacca gatcgcggat ctcctggatg aagtcgcggt ccaggctgaa cagcctcggc
     1981 accaggttcg ggatgtactt gccgatgatc aggtccggat ccttgtcgcg cggcatgacc
     2041 cgctccagcg accgccggaa gtcggcaacg ccgacgcgca gcacggatga gtggaacggc
     2101 acgtcgatgc ccggcacgag gatgaacgac cgcttgccgc cggagatctc gcggcgccgc
     2161 tcgacctctt cctcgagcgc ttccagaccg cgcactgtgc ccgcgatggc gtactgcgag
     2221 ccacgcaggt tgaagttgac gatttccagg aattcacctg tgcgctcgga aatatcggcc
     2281 acgaaggcct tcacgtcggc gtcgtcgaga tcgatctgcg acggccggat ggcggccaac
     2341 cggtagttgg accgacccag gtgatcgcgc ggcacgatgt cgtgcatctt gcttccgcgg
     2401 tggaacacga cctccagcag cgcctccagt tcgtagacac cggacacgca agcgagcgcg
     2461 gtgtactcac cgacggagtg gccgcaggcg atcgcgccct cgacgaaggc accctgctca
     2521 cgcatctcgg ccacctgcgc ggcagccacg gtcgccatcg cgacctgagt gaactgcgtc
     2581 aagaacagca cgccatcggg gtggtggtag tgcacaccgc tggcgatcag gctggtcggg
     2641 ttgtcgcgca ccacgtgtag caccgagaag cccaacgtgt cgcgagtgaa cttgtcggcg
     2701 gtgtcccaca ccttgcgggc ggccttggac cgggcgcgga cctccatgcc catgcccttg
     2761 gactggatgc cctgacc
//
LOCUS       DASRJA010000627         6149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2333922,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000627 DASRJA010000000
VERSION     DASRJA010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2333922"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2211)
                     /gene="secA"
                     /locus_tag="VFC01_28120"
     CDS             complement(<1..2211)
                     /gene="secA"
                     /locus_tag="VFC01_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZN83520.1"
                     /translation="MLSKLLRLGEGRMVKRLKGVADYVNTLSDDVEKLSDAELRAKTD
                     EFKKRVADGTDLDDLLPEAFAVAREAAWRVLDQRHFDVQVMGGAALHFGNVAEMKTGE
                     GKTLTAVLPSYLNALSGNGVHVVTVNDYLAKRDAEWMGRVHRFLGLGVGVILSGMTPD
                     ERRASYAADITYGTNNEFGFDYLRDNMAHSLADLVQRGHNFAIVDEVDSILIDEARTP
                     LIISGPADGSSNWYTEFARLAPLMEKDTHYEVDLKKRTIGVHEVGVELVEDQLGIDNL
                     YEAANSPLVSYLNNSIKAKELFQRDKDYIVRDGEVLIVDEFTGRVLIGRRYNEGMHQA
                     IEAKEHVEIKAENQTLATITLQNYFRLYDKLSGMTGTAQTEAAELHEIYGLGVVSIPT
                     NKPMVRADQSDLIYKTEEAKYIAVVDDVAERYEKGQPVLIGTTSVERSEYLSRQFQKR
                     RIPHNVLNAKYHEQEAGIIAEAGRLGAITVATNMAGRGTDIVLGGNVDFLADKRLRDQ
                     GLDPVETPEDYEAAWHEVLPQVKADAADEAKDVVAAGGLYVLGTERHESRRIDNQLRG
                     RSGRQGDLGESRFYLSLGDELMRRFNGETLESLLNRLNLPDDVPIEAKMVSRAIKSAQ
                     TQVEQQNFEIRKNVLKYDEVMNQQRKVIYEERRLILEGENLAEQAHKMLIDVITAYVN
                     GATAEGYSEDWDLEQLWTALKQLYPVGIDHHDLIDSDAVGEAGELTRDELLDALV"
     gene            complement(2287..2934)
                     /gene="raiA"
                     /locus_tag="VFC01_28125"
     CDS             complement(2287..2934)
                     /gene="raiA"
                     /locus_tag="VFC01_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZN83521.1"
                     /translation="MVIEDETAEPTPNAEVVVKGRNVEVPDHFRIYVSEKLSRLERFD
                     RTIYLFDVELDHERNRRQRKNCQHVEITARGRGPVIRGEACADSFYAALESAVCKLES
                     RLRRHKDRRKIHYGDKTPVSLAEATKIEPESFQPPASLAEENLQVDGVAVDDHEPGRV
                     VRTKEHPATPMTVDDALSQMELVGHDFFLFHDKQSDKPCVVYRRHAYDYGLIRLA"
     gene            complement(3256..3885)
                     /locus_tag="VFC01_28130"
     CDS             complement(3256..3885)
                     /locus_tag="VFC01_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HZN83522.1"
                     /translation="MLDLVLPLECGGCGAPSTRWCPACAQELAVKPDQPHVITPRIDP
                     GVPVLSLGRYAGSRREAIVAVKEHGRADLIAPLAVALRAGLQRLLAWGVVGTPLTIVP
                     APTRRSAARRRGGDPVTRMARTATAGMGGIEVVQALRLRSMVRDSVGLSSADRQRNIA
                     GRVKIIKPVAANVLVVDDIVTTGATAAESVRVLHIAGAHAAGVLAIANA"
     gene            3937..5244
                     /locus_tag="VFC01_28135"
     CDS             3937..5244
                     /locus_tag="VFC01_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HZN83523.1"
                     /translation="MQDKRESFIVDCLVNAFSDLMAADPDAFRTKFRKMAADPFAFYR
                     GSACVFYADVADRKDRWADERTSRVWIQGDLHAENFGTYMDGSGVLIFDVNDFDEAYV
                     GHFTWDLQRFAASVALMCWQKAISDEEITALIETFVRAYVRQVRWFVDVEDDSSFSLN
                     LETARGAVLSALQSARLSTRAQMLDRMTRVDEWDRVFRDSQGARQLDDAERDKVADAF
                     DRYLETIPKTKRFRGIAYDIKDVIGKTGLGIGSAGLPAYTVLIEGFNQALDNDVVLSI
                     KQGNVAAPSRVVDDPDVREYFTHHGHRTAVSQRALQAHADPLLGYTDIDGVGYVVSEI
                     SPYDADLDWSELTEPDELTEVIEQLGQAVAKVHCVSDTDSDQTLVEFQTEEAIVDVIG
                     DDTDDFVADIVAFGLEYAGTVRDDHRHFVEAFREGRIPGVSST"
     gene            complement(5248..5685)
                     /locus_tag="VFC01_28140"
     CDS             complement(5248..5685)
                     /locus_tag="VFC01_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZN83524.1"
                     /translation="MHRKQFLLGAGLGVVTAVVAACSTYGKKPEAAGESGTTTTAAPA
                     TPGGTAAPAANVIAKTADVPVGSGVIVDEVVVTQPTAGVFKGYSAKCTHKGCMVDKIA
                     DGTIDCPCHGSKFNLDGTVANGPADKPLPPENITVQGDEIVLS"
     gene            complement(5699..>6149)
                     /locus_tag="VFC01_28145"
     CDS             complement(5699..>6149)
                     /locus_tag="VFC01_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010591999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6529 family protein"
                     /protein_id="HZN83525.1"
                     /translation="EPQFFSINVAEFSSGLAVKSWLATVAMLLALFQLGSAFAMYRLI
                     PGGKAPAWIGTAHVWSGRLAVLASVPVAVHCLYALGFESYDNRVLFHSLFGCFFYGAF
                     VTKMVLLTRKGLRGWVIPIAGGLVFFGLVYVWLTSALWFFQTNGIRF"
BASE COUNT         1018 a         1968 c         2025 g         1138 t
ORIGIN      
        1 gaccaacgca tcgagcagct cgtcgcgcgt cagctcgccc gcctcaccga ccgcgtcgga
       61 gtcgatcagg tcgtggtggt cgatgccgac cgggtagagc tgtttgagcg ccgtccacag
      121 ctgctcgagg tcccagtctt ccgagtagcc ctcggccgtg gcgccgttga cgtaggcggt
      181 gatgacgtcg atgagcatct tgtgggcctg ctcggcgagg ttctcccctt cgaggatcag
      241 gcggcgctcc tcgtagatga ccttgcgctg ctggttcatc acctcgtcgt acttgaggac
      301 gttcttgcgg atctcgaagt tctgctgctc cacctgtgtc tgcgcgctct tgatcgcgcg
      361 ggagaccatc ttggcctcga tcggtacatc gtccggcaga ttcagccggt tgagcagcga
      421 ctccagcgtc tcgccgttga accgacgcat cagctcatca ccgagtgaca ggtagaaccg
      481 cgactcgccg aggtcaccct ggcggcccga gcggccgcgc agctggttgt cgatgcggcg
      541 ggactcgtga cgttcggtgc caagcacgta caggccgccg gcggcgacga cgtccttggc
      601 ctcgtcggcc gcgtcggcct tgacctgcgg cagcacctcg tgccaggcgg cctcgtaatc
      661 ctccggcgtc tcgaccgggt cgaggccctg gtcgcgcagc cgcttgtcgg cgaggaagtc
      721 gacgttgccg ccgagcacga tgtcggtgcc gcggccggcc atgttggtcg ccacggtgat
      781 cgcacccagc cggccggcct cggcgatgat gccggcctcc tgctcgtggt acttcgcgtt
      841 gagcacgttg tgcgggatcc gccgcttctg gaactggcgc gacaagtact ccgagcgctc
      901 gacgctggtg gtgccgatca agaccggctg acccttctcg tagcgctcag ccacatcgtc
      961 gacgaccgcg atgtacttgg cctcttcggt cttgtagatc agatcggact ggtcggcgcg
     1021 aaccatcggc ttgttggtgg ggatgctcac cacgccgagg ccgtagatct cgtgcagctc
     1081 ggccgcctcg gtctgggcgg tacccgtcat gccggaaagc ttgtcgtaga gccggaagta
     1141 gttctgcagc gtgatggtgg ccagggtctg gttctcggcc ttgatctcga cgtgctcctt
     1201 ggcctcgatg gcctggtgca tgccctcgtt gtagcgacgg ccgatcaaca cgcggccggt
     1261 gaactcgtcg acgatgagga cctcgccgtc gcggacgatg tagtccttgt cgcgctggaa
     1321 cagctccttg gccttgatcg agttgttgag gtagctgacc agcggcgagt tggccgcctc
     1381 gtagagattg tcgataccga gctggtcctc gaccaactcg acgccgacct cgtgcacacc
     1441 gatggtgcgc ttcttcaggt cgacctcgta gtgggtgtcc ttttccatca gcggcgccag
     1501 ccgcgcgaac tcggtgtacc agttcgacga gccgtcggcg ggcccggaga tgatcagcgg
     1561 ggtgcgggcc tcgtcgatca ggatggagtc gacctcgtcg acgatcgcga agttgtgacc
     1621 gcgctgcacg aggtcggcca gcgagtgcgc catgttgtcg cgcagatagt cgaagccgaa
     1681 ctcgttgttc gtgccgtagg tgatgtcggc cgcgtaggag gcgcgccgct cgtcgggcgt
     1741 catgccggac aggatcacgc cgacgcccag cccgaggaag cggtgcacgc ggcccatcca
     1801 ctcggcgtcg cgtttggcca ggtagtcgtt gacggtcacg acgtgcacgc cgttgccgga
     1861 cagcgcgttc aggtacgacg gcagcaccgc ggtcagggtc ttgccctcgc cggtcttcat
     1921 ctccgcgacg ttgccgaagt gcagcgccgc gccgcccatc acctggacat cgaaatgccg
     1981 ctgatcgagc acccgccagg ccgcctcgcg ggccaccgcg aacgcctcag gcagcaggtc
     2041 gtcgaggtcg gttccgtcgg cgacgcgctt cttgaactcg tcggttttgg cccgcagctc
     2101 ggcgtcggag agtttttcca cgtcgtcgga cagggtgttg acgtagtcag ccaccccctt
     2161 gaggcgcttg accatgcggc cttcaccgag acggagcaac ttcgacagca cgctatcccc
     2221 tatgggttgg atttcttgag ctcaacccat ggtaggtgac acggcctgcg ccccggccga
     2281 acagcgtcag gccagacgaa tcagtccgta gtcgtaggcg tgcctgcgat acaccacgca
     2341 gggtttgtcg ctctgtttgt cgtggaacag gaagaagtcg tggccgacga gctccatctg
     2401 ggaaagcgcg tcatcgacgg tcatcggggt cgccggatgc tccttggtgc gcaccacccg
     2461 gccgggctcg tgatcgtcga ccgccacccc gtcgacctgc aggttttcct cagcgagcga
     2521 tgcgggcggc tgaaatgact cgggctctat cttggtggcc tcggccagcg agacgggcgt
     2581 cttgtccccg tagtggatct tgcggcggtc cttatggcgg cgcagcctgc tctccagttt
     2641 gcagaccgcc gactcgaggg cggcgtagaa gctgtcggcg caggcctcgc cgcggatgac
     2701 ggggccgcga cctcgcgcgg tgatctctac gtgttgacag ttcttgcgtt ggcgccggtt
     2761 gcgctcatgg tccagttcga cgtcgaagag gtagatggtg cggtcaaatc gttccaggcg
     2821 ggacagtttt tctgagacat agatgcggaa gtgatcagga acctcaacgt ttcggccctt
     2881 gacgacgacc tcggcgttcg gagtgggttc agctgtctcg tcctcgatca ccatggtgcg
     2941 gtctgagtcc acggaatggc ttgacatgct tggcaactcg tttctctacg gagtcgcacg
     3001 cttcgcgtgc ccggcttgtc ttgggaatcg cgccggtggg cctgcaatgt cgctccgtcc
     3061 gccggcgcaa ggtgtcgagt actcacctcc taccgctggt cggcgatgct ggcgcttcgg
     3121 gttgagcgcc gccgtgaggc attcacgggt ggttggtgcc gacggtaatc gtgttcaccc
     3181 ggtcgtgcca gtaaattcgc gcatgcgttt tcagttcttc acgtccgatt gatgtcgggg
     3241 taatcgccgg agctgtcaag cgttggcgat ggcaaggacc ccggccgcgt gagcaccggc
     3301 gatgtgcagg acgcggaccg attcggcggc agtggcgccg gtggtcacga tgtcgtcgac
     3361 gacgaggacg tttgcggcta ccggcttgat gatcttgacg cgtccggcga tgttgcgctg
     3421 acggtcggca ctggacaagc cgacggagtc gcggaccata ctccgcagcc tcagcgcctg
     3481 aacgacctcg ataccgccca tgcccgccgt cgccgtccgc gccatccggg tgacgggatc
     3541 gccgccgcgg cgccgggccg cggaccggcg ggtcggcgcg ggcacgatgg tcagcggtgt
     3601 gccgaccacg ccccacgcaa gcagacgctg caggccggct cgcaacgcga cggcgagcgg
     3661 cgcgatcagg tcggcgcggc catgctcctt gaccgcgacg atcgcttcgc ggcgcgaccc
     3721 ggcgtagcgg ccgagcgaca acaccggcac accgggatcg attcgcgggg tgatgacgtg
     3781 cggttggtcg ggcttgaccg ccagctcctg agcgcaggcg gggcaccatc tggtcgaggg
     3841 ggcgccgcag ccaccgcact cgagcgggag gacgaggtcg agcacggcgc cattgtcgcg
     3901 gcgatgaccg acattgattg cgggtactcc gtggccatgc aagacaaacg cgagtcattc
     3961 atcgtcgact gcctcgtcaa cgcgttctcc gacctgatgg cggccgatcc cgacgcgttt
     4021 cggaccaagt tccgaaagat ggcggccgac ccgttcgcgt tctaccgcgg cagcgcatgc
     4081 gtgttctacg ccgacgtcgc cgaccgcaag gacaggtggg ccgacgaacg gacttcgcgg
     4141 gtgtggatcc agggcgacct gcacgcggag aacttcggca cctacatgga cggctcaggt
     4201 gtgttgatct tcgacgtcaa cgatttcgac gaggcctatg tcggccactt cacctgggac
     4261 ctgcagcggt tcgccgccag cgtcgcgctg atgtgctggc agaaggcgat atccgacgaa
     4321 gagatcacgg cgctgatcga aacgttcgtc cgcgcctacg tccgccaggt ccggtggttc
     4381 gtcgacgtcg aggacgactc gtcgttctcg ctgaacctcg agacagctcg cggtgcggtg
     4441 ttatcggcgt tgcagtcagc acgcctgtcg accagggcgc agatgctgga ccgcatgacc
     4501 cgagtagacg agtgggacag ggtttttcgc gattcgcagg gcgcacgcca gctggacgat
     4561 gccgaacgcg acaaggtggc cgacgcgttc gaccgctatc tggagacgat cccgaaaacc
     4621 aaacgcttcc gcggcatcgc ctatgacatc aaggacgtca tcggcaagac cggactcggg
     4681 atcggcagcg ccgggctgcc cgcctacacc gtgctgatcg agggcttcaa tcaggcgttg
     4741 gacaacgacg tcgtattgtc gatcaaacag ggcaatgtgg ccgcgccgag ccgggtggtc
     4801 gatgacccgg atgtgcgcga gtacttcacg caccacggcc atcgcaccgc cgtgtcgcag
     4861 cgggcactgc aggcccacgc cgatcccctg ctcggctaca ccgacatcga cggggtcggc
     4921 tacgtggtca gcgaaatttc gccctacgac gccgatttgg actggagcga gttgacggag
     4981 ccggacgagc tcacagaagt catcgagcaa ctcgggcagg cggtggccaa agtgcactgc
     5041 gtcagcgaca cagacagtga ccagactttg gtcgagttcc aaaccgagga ggccatcgtc
     5101 gacgtgatcg gcgacgacac cgacgacttc gtggccgaca tcgtcgcttt cgggctggag
     5161 tacgccggaa ccgtgcggga cgatcaccgc cacttcgtcg aggcgttccg cgaaggccgg
     5221 atcccgggag tcagttcaac ctgaccgcta gctcagcact atctcatcgc cctgcacagt
     5281 gatgttctcg ggcggcaacg gcttatcagc cggtccattg gccaccgtgc cgtcgaggtt
     5341 gaacttgctg ccgtggcacg ggcagtcgat ggtgccgtca gcgatcttgt ccaccatgca
     5401 tcccttatgg gtgcacttcg cggagtaccc tttgaacaca cccgcggtcg gctgtgtcac
     5461 cacgacctcg tcgacgatca cgcccgaccc gacaggcaca tcggccgtct tggcgatgac
     5521 gttcgccgcc ggcgcggcgg tgccgcccgg tgtggccggg gcggcggtcg tcgtcgtgcc
     5581 ggattcgcct gccgcttcgg gcttcttgcc gtatgtgctg caggccgcaa ctacggcggt
     5641 caccacgccc aggccggcgc cgaggaggaa ctgtttgcga tgcatggcag tcctttcgct
     5701 agaacctaat tccgttggtt tggaagaacc acagtgccga ggtcagccag acgtagacca
     5761 agccgaagaa caccaacccg cccgcgatcg gaatcaccca tccccgaagg cctttgcggg
     5821 tgagcagcac catctttgtc acgaaggcgc cgtagaagaa gcagccgaac agcgaatgga
     5881 acagcacccg gttgtcatag gactcgaagc ccagcgcgta caggcagtgc accgcgacgg
     5941 ggacgctggc cagcaccgcc aaccgccccg accacacatg cgcggtgccg atccacgccg
     6001 gcgcctttcc accgggaatc agccggtaca tcgcgaatgc ggaccccagc tggaacagcg
     6061 ccagcaacat ggccaccgtc gccaaccatg acttgacggc cagcccgctg gagaattcgg
     6121 ccacattgat cgaaaagaac tgtggctca
//
LOCUS       DASRJA010000628         8319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2313583,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000628 DASRJA010000000
VERSION     DASRJA010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2313583"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VFC01_28150"
     CDS             <1..469
                     /locus_tag="VFC01_28150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MspA family porin"
                     /protein_id="HZN83526.1"
                     /translation="RNRLTREWFHSGSAVYNVGGPGADAFEGTLELGYQIGFPWSLGV
                     GINFSYTTPNILLDDTNINPLSVGFNPLGSVITPNLFPGVSISADLGNGPGIQEVATF
                     SVDVAGPHGKVAVSNAHGTVTGAAGGVLLRPFARLISKAGDSVTTYGEPWNMN"
     gene            634..1278
                     /locus_tag="VFC01_28155"
     CDS             634..1278
                     /locus_tag="VFC01_28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MspA family porin"
                     /protein_id="HZN83527.1"
                     /translation="MKAISRVLIAMVAAIAALFVSTGTSHAALDNEMSLVDGQDRTMT
                     IQQWDTFLNGVFPLDRNRLTREWFHSGSAVYDVAGPGADAFEGTLELGYQIGFPWSLG
                     VGINFSYTTPNILLDDTNINPLSVGFNPLGSVITPNLFPGVSISADLGNGPGIQEVAT
                     FSVDVAGPHGKVAVSNAHGTVTGAAGGVLLRPFARLISKAGDSVTTYGEPWNMN"
     gene            complement(1275..1733)
                     /gene="mscL"
                     /locus_tag="VFC01_28160"
     CDS             complement(1275..1733)
                     /gene="mscL"
                     /locus_tag="VFC01_28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HZN83528.1"
                     /translation="MLKGFKEFLSRGNIVDLSVAVVIGTAFTALVTKFTDSIIQPLIN
                     RIGAGKDSDYGILRIGIGGGQSIDLNILLSAAINFVLVAAVVYFLVMVPYERLRKKGE
                     VEQAQDTELSLLTEIRNILASTDGQSGETVTGPGTGPSPDTAKHTSADRS"
     gene            1868..2068
                     /locus_tag="VFC01_28165"
     CDS             1868..2068
                     /locus_tag="VFC01_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83529.1"
                     /translation="MRWLWSAVAVIGLSVFAIGTASAEPTQPPAPGPTSTSDELADMV
                     LDAIQHGNPAAPTTTPVPAPPR"
     gene            complement(2074..2733)
                     /locus_tag="VFC01_28170"
     CDS             complement(2074..2733)
                     /locus_tag="VFC01_28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HZN83530.1"
                     /translation="MGDSLNPSVVTRLLAALRPDWSRTLAARRIAAGALVILAAVIAL
                     RSDPQGDHTEIVVAARDLSSGVELTADDVRLETRTAATVPDGAQSDVGAIIGTTLAGP
                     ARRGEVLTDVRVLGPRLAESVAGPDARIVPLPLSDTALLDLVRPGDVVDVLAAGPESS
                     GADAQPQLVATNAVVVLVSDKPKGAGSGSDRVVLVAVAAHAANDVAGAALLQNVTLTF
                     H"
     gene            complement(2799..3131)
                     /locus_tag="VFC01_28175"
     CDS             complement(2799..3131)
                     /locus_tag="VFC01_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HZN83531.1"
                     /translation="MPTYSYACTECGDRFDAVQAFSDSALTTCAKCNGRLRKLFGNVG
                     VVFKGSGFYRTDSRESAKSSSNGSATTSESTSSSPSEKSSEKSSSTSTNGSSSSSTTA
                     APAAAASS"
     gene            complement(3204..3794)
                     /locus_tag="VFC01_28180"
     CDS             complement(3204..3794)
                     /locus_tag="VFC01_28180"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZN83532.1"
                     /translation="MAPATKTELRAEILMTRRTLAPQLHDAEAHALCGHLPTFIGDGE
                     TVCAYVPVGSEPGSAELIDSLLRRGVRVLLPVARHDQAGLPAPLQWGEYRPGGLVQAR
                     FGLREPVEPWLPADAIASATVVLVPALAVDRAGVRLGRGAGFYDRSLPLATPTARLVA
                     VVRDDELVDRLPAEPHDVRMTHALTPRAGLVALTWE"
     gene            3890..4801
                     /locus_tag="VFC01_28185"
     CDS             3890..4801
                     /locus_tag="VFC01_28185"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HZN83533.1"
                     /translation="MTRSLVPIPHTAVVPAAGLGTRFLPATKTVPKELLPVVDTPGIE
                     LVAAEAAEAGAERLIIITSEGKDGVVAHFVEDLVLEGTLEARGKKSMLEKVRRAPALI
                     KVESVVQAEPLGLGHAIGCVEDSLSDDEDAIAVLLPDDLVLPTGVLETMSKVRAKRGG
                     TVLCAIEVEPDEISAYGVFDVEPVPDTNNPNVLRVKGMVEKPKAEDAPSPYAAAGRYV
                     LDRAIFDALRRVPRGAGGEIQLTDAVALLIKEGHPVHVVVHRGSRHDLGNPGGYLKAA
                     VDFALKRDDYGPELRRWLVERLGLIDC"
     gene            4839..6101
                     /gene="glp"
                     /locus_tag="VFC01_28190"
     CDS             4839..6101
                     /gene="glp"
                     /locus_tag="VFC01_28190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZN83534.1"
                     /translation="MRSVEEQQARVAAAAVAPRPVRVAIAEAQGLMCAEEVVTERPLP
                     GFDQAAIDGYAVRSVDVLGVGDDDSNSDEDDNREISLPVMASIDAGTRTPSRLQPRQA
                     ARVQTGAPMPTLADAVLPLRWTDGGESRVTVLRGVRSGAYVRRTGDDVQPGDVAVRAG
                     TIIGPAQVGLLAAVGRDRVLVHPRPRLSVMCVGGELVDISRTPGNGQVYDVNSYALAA
                     AGRDAGAEVNRVGIVDTDPKELREIVEGQVNRAEVVVIAGAVGGAAAEGVRSVLAELG
                     EMEVTRIAMHPGSVQGFGQLGPDGVPVFLLPANPVSALVVFEVMVRPLIRLSLGKRQP
                     MRRVVQARTLSPITSVAGRKGYLRGQLMRDQDTGEYLVQALGGAPGASSHLLATLAEA
                     NCLVVVPSEAEQIRTGEIVDVAFLAQRG"
     gene            6122..6775
                     /locus_tag="VFC01_28195"
     CDS             6122..6775
                     /locus_tag="VFC01_28195"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZN83535.1"
                     /translation="MNLWRSSSLHPGWPSPVGPLRVPAGLVRLRPVRLRDASQWSRIR
                     LADRAHLEPWEPATDVNWEVRHAVSSWPSVCSGLRSEARKGRMLPYVIELDGEFAGQL
                     TIGNVTHGALRSAWIGYWVASSATGGGVATAALALGLDHCFGPVMLHRVEATVRPENA
                     ASRAVLAKVGFREEGLLRRYLEVDGSWRDHLLVAITVEELNGSVASALVREGHASWA"
     gene            6931..7962
                     /gene="glpR"
                     /locus_tag="VFC01_28200"
     CDS             6931..7962
                     /gene="glpR"
                     /locus_tag="VFC01_28200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase receptor GlpR"
                     /protein_id="HZN83536.1"
                     /translation="MPSIPQSLLWISLVVLWLFVLVPMLISKRDAVRRTSDVALATRV
                     LNTGRAARLLRRGGPAAGHRSDPDWQPSDEDLDEDHDDELHDESAERSVVRVAAVETE
                     VDSEPDYLDVDVVDEDSGALPVGASANEAVNEEIEERQDQTDELPLDFGATETEDVQE
                     EDVHEDDEPDGTDDEYEYVADSSGLEAPSDTDLQLAESISAARKRRYESKTSAAVTVR
                     KYKFRKRMLTVMTLATIAAAAAAYTVAPVAWWGCGSLGAMTMLYLAYLRRQTRIEERL
                     RRRRAQRMARARLGVENTDDREFDVVPSRLRRPGSVVLEIDDEDPFFEHLDYAPFARD
                     IDLPRAAGQ"
     gene            8009..8081
                     /locus_tag="VFC01_28205"
     tRNA            8009..8081
                     /locus_tag="VFC01_28205"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8042..8044,aa:Ala,seq:cgc)
BASE COUNT         1375 a         2598 c         2919 g         1427 t
ORIGIN      
        1 ccgcaaccgg ttgacccgcg agtggttcca ctccggcagt gccgtgtaca acgtgggcgg
       61 cccgggtgcc gatgcgttcg agggcacgct ggagctgggt taccagattg gtttcccctg
      121 gtcgttgggt gtgggtatca acttcagcta caccacgccg aacatcctgc ttgatgacac
      181 gaacatcaac ccgctgagtg tcggcttcaa cccgctgggt tcggtcatta ccccgaacct
      241 gttcccgggt gtgtcgatca gcgccgatct gggtaacggt cccggtattc aggaggtcgc
      301 gaccttctcg gttgacgtgg cgggtccgca cggcaaggtg gcggtctcca atgctcacgg
      361 cacggtgact ggagcggccg gtggtgtgtt gctgcgtccg ttcgcccggc tgatctccaa
      421 ggccggcgac agcgtcacca cctacggcga gccctggaac atgaactaac tcccagctca
      481 gggcataaga agcagcctcc ggcattttgc cgggggctgt tttttcgtta accaccacag
      541 caacgagaga aatcagctac aacatctgta acggttgcat caccgtttgc gatcacccgt
      601 tgtaacgcca ggcccttaag agatggggaa cacatgaagg caatcagccg ggtgctgatc
      661 gcgatggtag cggcgattgc cgcgctgttc gtgagcactg gcacctctca cgcagctctg
      721 gataacgaga tgagtctggt cgacggtcag gaccggacga tgacgatcca gcagtgggac
      781 accttcctga atggtgtgtt ccccttggac cgcaaccggt tgacccgcga gtggttccac
      841 tccggcagtg ccgtctacga cgtggccggc ccgggtgccg atgcgttcga gggcacgctg
      901 gagctgggtt accagattgg tttcccctgg tcgctgggtg tgggtatcaa cttcagctac
      961 accacgccga acatcctgct tgacgacacc aacatcaacc cgctgagcgt cggcttcaac
     1021 ccgctgggct cggtcattac gccgaacctg ttcccgggtg tgtcgatcag cgccgatctg
     1081 ggtaacggcc cgggtattca ggaggtcgcg accttctcgg ttgacgtggc gggtccgcac
     1141 ggcaaggtgg cggtctccaa tgctcacggc acggtgactg gtgcggccgg tggagtgttg
     1201 ctgcgtccgt tcgcccggct gatctccaag gccggcgaca gcgtcaccac ctacggcgaa
     1261 ccctggaaca tgaactagga cctgtcggca ctggtgtgct tggcggtgtc ggggcttggt
     1321 cccgtgccgg ggccagtgac cgtctcgccg gactgcccat cggtggaggc gaggatgttg
     1381 cggatctcgg tgagcaggct cagctcggtg tcctgggcct gctcgacctc gcccttcttg
     1441 cgaagccttt cgtacgggac catgaccagg aagtagacca ctgcggcgac aaggacgaag
     1501 ttgatggccg ccgacagcag gatgttcaga tcgatagact ggccgccgcc gatgccgatc
     1561 cgcaggatgc cgtaatcgga gtctttgccg gcgccgatgc ggttgatcaa cggctggatg
     1621 atgctgtcgg tgaatttcgt gaccaacgcc gtgaacgcgg taccgatgac cacagcgacg
     1681 gataagtcga cgatgttgcc cctggacaga aactccttga agcccttcaa catgcgctcg
     1741 aatccctcct gcattcgtgt gctgcctgac aagccctgac gttagtcgcg ttgagcgtgc
     1801 ggcggatgcg gtccggtgct agcttcgccg gggaatcaag ctgtgtacca aaggaaagcg
     1861 agtggcgatg cggtggctgt ggtcggcggt ggcggttatt gggctctcgg tgttcgcgat
     1921 cggcacggcc agcgcggaac cgacgcagcc ccctgcgccg ggacccacat cgacctccga
     1981 cgagttggcc gacatggtgt tggacgcgat ccagcacggc aatccagcgg ccccgaccac
     2041 caccccggtg cctgcgccgc cgcgatgacg ggatcagtgg aaggtaagcg tgacgttctg
     2101 tagcagcgcc gcgcccgcca cgtcgttggc ggcgtgagcg gcgacggcca ccagcaccac
     2161 acggtcgctt ccgctgcctg cgcccttcgg cttgtccgac accagcacca cgacggcgtt
     2221 ggtcgctacc agctgaggtt gggcatccgc gccgctggat tcgggacccg cggcgagcac
     2281 gtccaccacg tcaccgggcc ggaccagatc cagcagcgcg gtgtcggaca gcggcagcgg
     2341 cacgatgcgt gcatccgggc ctgccaccga ttcggccagc cgcggaccca acacgcgcac
     2401 gtcggtcagc acctccccgc gacgagccgg gccggccagc gttgtaccga tgatcgcgcc
     2461 gacgtccgat tgtgcaccat cgggaaccgt tgccgccgtg cgggtttcga gacggacatc
     2521 gtccgcggtc agttcgacac ctgaggacag atcgcgggcc gcgacgacaa tttcggtgtg
     2581 gtcaccctga gggtccgagc gcagtgcgat gacggcggcc aggatgacga gcgcgcccgc
     2641 ggcgatccgg cgggcggcga gcgtacgcga ccagtccggt cgcagcgcgg ccagcagccg
     2701 ggtcacgacc gatggattga gcgagtcccc catgccgcca cgctaggcgg cgaaccgccg
     2761 cgtcggcgca agcgcgggcc gcgcctgtgg ataacgggtc agctggatgc ggccgcagcg
     2821 ggcgccgcgg tagtggagct ggagctcgac ccgttggttg aggtggagct ggacttttcg
     2881 gacgacttct cggacggcga tgagctggtg gactccgagg tggtggcgga gccgttggag
     2941 gagctcttgg ccgattcgcg gctatcggtg cggtagaagc cgctgccctt gaacaccact
     3001 cccacgttgc cgaacagctt gcgcagccgg ccgttgcact tggcgcaggt ggtcagcgcg
     3061 ctatcgctga acgcctgcac cgcgtcgaac cgatcgccgc actcggtgca cgcataggaa
     3121 taggtaggca cgaaaacctc cgaggtggtc aaacttctta gcactctacc ggctcaagtg
     3181 ctagaaccgc tatgtgggct cgatcattcc cacgtcagcg ccaccaagcc ggcccgcggt
     3241 gtcagcgcat gtgtcatccg cacgtcgtgc ggttcggcgg gcaaccggtc gacgagctcg
     3301 tcgtcgcgga ccacagccac caaccgtgcc gtcggagtgg ccagtggcag cgatcgatcg
     3361 tagaaccccg cgccccgccc cagccgcacc ccggcgcggt ctaccgcgag cgcaggcacc
     3421 aacaccactg tggcggaagc gatcgcgtca gcaggcagcc atggctcgac gggctcgcgc
     3481 aatccgaacc gcgcctgaac caggccgccg gggcggtact cgccccactg cagcggcgcg
     3541 ggcaggcctg cctggtcgtg acgggccacc ggcagcagca cccgtacgcc gcgccgcagc
     3601 aacgaatcga tcagctcggc cgagcccggt tcggagccca ccggcacgta cgcgcagacc
     3661 gtttcgccgt cgccgatgaa cgtcggcagg tgcccacaca gcgcgtgggc ctcggcgtcg
     3721 tgcagctgtg gcgccagcgt gcggcgggtc atcaggatct cggctcgcag ttcggtcttt
     3781 gtcgcaggcg ccacgcgtcc acgatgacag cctcttcgca gcagtcgcaa aacgaacgca
     3841 actgagggct tcgcggctta tcggagctaa tgccgttagt gtgtgcagga tgacacggtc
     3901 cctggttccg attccgcaca ctgcggtcgt ccctgctgcg ggtctgggga cccggtttct
     3961 gccagcaacc aagaccgtgc ccaaggagtt gctgcccgtc gttgacaccc ccggcatcga
     4021 actggtggct gccgaggcgg ccgaagcggg ggccgagcgg ctgatcatca tcacctccga
     4081 gggcaaggac ggggtcgtcg cgcacttcgt cgaagacctc gttctcgagg gcacgctgga
     4141 ggctcgcggc aagaagtcga tgctggagaa ggtgcgccgc gcaccggccc tgatcaaggt
     4201 ggagtcggtg gtccaggccg aaccgctcgg cctggggcac gccatcggtt gcgtcgagga
     4261 cagcctgtcc gacgacgaag acgcgatcgc ggtcctgctg cccgatgacc tggtgctgcc
     4321 gaccggcgtg ctggagacca tgtcgaaggt gcgtgccaag cgcggcggga cggtgctatg
     4381 cgccatcgag gtcgagcccg acgagataag tgcgtacggc gtttttgacg tcgagccggt
     4441 accggacacg aacaacccga acgtattgcg ggtcaagggg atggtggaga agcccaaggc
     4501 cgaggacgcg ccgtcgccgt atgcggcggc cggccgctac gtgctcgacc gcgcgatttt
     4561 cgatgcgctg cggcgggttc cgcggggagc gggcggtgag atccaattga cggacgcggt
     4621 cgcattgctg atcaaagaag ggcatccggt gcatgtggtg gtgcaccgcg ggtcccgaca
     4681 cgaccttgga aatcctggcg gctatctcaa ggctgcggtt gactttgcgt tgaagcgcga
     4741 cgactacggc cccgaattgc ggcggtggtt ggtggagcga ttggggctga tcgactgctg
     4801 atacgccgcg gtggccctga acggcagaaa ggcgtgcagt gcgttcggtg gaggagcaac
     4861 aggcccgggt agcggccgcc gcggtggcgc cacgaccggt ccgggtcgcg atcgccgagg
     4921 cacaggggtt gatgtgcgcg gaggaggtcg tcaccgagcg cccgctgccg ggattcgatc
     4981 aggccgccat cgacggctac gcggtgcgca gcgtcgacgt gctgggcgtc ggtgacgacg
     5041 actcgaattc cgatgaggac gacaaccgcg aaatcagctt gccggtgatg gcgtcgatcg
     5101 acgccggcac ccgcacgccg agtcggctgc agccgcgcca ggccgcgcgc gtgcagacgg
     5161 gtgcgccgat gccgacgctg gccgatgcgg tgctgccgtt gcggtggacc gacggcggtg
     5221 agtcgcgggt gacggtgttg cggggcgtgc gttccggcgc ttacgtccgc cgcaccggcg
     5281 acgacgttca gccgggcgac gtcgcggtgc gggccggcac aatcatcggt ccggcacagg
     5341 tgggcttgct cgcggctgtt ggccgggacc gcgtgctggt gcatccgcgg cctcggctgt
     5401 cggtgatgtg cgtcggcggt gagctggtgg acatttcccg tacgcccggc aacgggcagg
     5461 tctacgacgt gaactcgtat gccctggctg cggcgggccg cgatgctggc gccgaggtga
     5521 accgcgtcgg catcgtcgac accgacccca aggaactgcg ggaaatcgtt gagggccaag
     5581 tgaatcgcgc agaggtggtg gtgatcgcgg gcgccgtcgg aggtgccgcc gccgaaggcg
     5641 tccggtcggt cctcgccgaa ctcggcgaaa tggaggtcac ccgcatcgcg atgcaccccg
     5701 gctcggtgca gggcttcggc caactgggac cagacggtgt gccggtgttc ctgctgccgg
     5761 ccaacccggt cagcgcgctt gtcgttttcg aggtcatggt gcggcccctg atccggctgt
     5821 cgctgggcaa gcgccagccg atgcggcggg tcgtgcaggc tcgcacactg tcgccgatta
     5881 cgtcggtcgc aggccgaaag ggctacctgc gcggccagct gatgcgcgac caggacaccg
     5941 gcgagtacct ggtgcaggcc ctgggcggcg ctccgggtgc gtcgtcgcac ctgctggcga
     6001 cgctcgccga agcgaactgt ttggtggttg tcccgagcga ggcggagcag attcgcaccg
     6061 gcgagatcgt tgacgtcgcg ttcctggcgc agcgcggctg accgaaaccg ggcgtgctgt
     6121 catgaacctg tggcgttcga gttctttgca tccgggatgg ccgagccctg tcggcccgct
     6181 gcgagtcccc gccggcctgg tgcggttgcg cccggtgcgg ctgcgcgacg cgtcacagtg
     6241 gagccgcatc aggctggccg accgtgcgca cctggaaccg tgggaaccgg ccaccgatgt
     6301 gaattgggaa gtgcgacacg cagtttcgtc gtggccgtcg gtttgttccg gactgcgctc
     6361 ggaggcccgc aagggtcgga tgctgccgta cgtcattgag ctcgacgggg agttcgccgg
     6421 gcagctgacc atcggcaacg tgactcacgg cgcgctgcgg tcggcgtgga tcggttactg
     6481 ggtggcgagt tcggcgaccg gcggcggggt ggcgacggcc gcgctggcgc tgggtcttga
     6541 tcactgtttc ggaccggtca tgctgcaccg cgtcgaggcg acggtgcgcc cggagaacgc
     6601 ggcgagccgg gccgtgctgg ccaaggtggg cttccgcgaa gaaggactgt tgcggcgata
     6661 cctcgaggtg gacggctcct ggcgcgacca cctgctggtg gccatcacgg tcgaggagct
     6721 caacggttcg gtagcgtccg cgctggtgcg cgagggccac gcgagctggg cctgacgccg
     6781 tctagcgaac cggcaaagct gttactgctg tgacatttgt tactgatggt gcttgtcagc
     6841 tgtgaattac aggtgtgtaa ttgacttcgg cgcgccgtcc ttggatgcgc tggtcacgga
     6901 cctagcctga aggggaaagg agcaggcgac atgccaagca tcccccaatc cctcctgtgg
     6961 atctccctcg tcgttctctg gctcttcgtg ctagtcccga tgctgatcag caagcgtgat
     7021 gccgttcgcc gcaccagcga cgtagccctg gcgacgcggg tgctcaacac cggtcgcgca
     7081 gcgcggctgc tgcggcgcgg cggtccggcg gccgggcatc gcagtgatcc ggactggcag
     7141 ccatcggacg aggatctcga cgaggaccac gacgacgaac tccatgacga gtcggcggag
     7201 cgttcggtgg tgcgtgtggc cgcggtcgag accgaggtcg acagcgagcc cgactacctc
     7261 gacgtcgacg tcgttgacga ggactcgggc gcgttgccgg tgggcgcgtc ggcgaatgag
     7321 gcggtcaatg aggagatcga agagcgccag gaccaaaccg acgagctgcc attggatttc
     7381 ggggccaccg agacggagga cgtgcaggaa gaggacgtgc acgaggacga cgagcccgat
     7441 ggcacggacg acgagtacga gtacgtcgcc gattcctccg gcctggaggc gccgtcggat
     7501 accgacctgc aattagccga gtcgatcagc gccgcgcgca agcgccggta tgagtccaag
     7561 acctcggccg cggtgaccgt gcgcaagtac aagttccgca aacggatgct gaccgtgatg
     7621 acattggcga ccatcgccgc ggccgcggcg gcgtacaccg tggcacctgt cgcgtggtgg
     7681 gggtgcggat cgctgggcgc catgacgatg ctctacctgg cgtacctgcg tcgtcagaca
     7741 cgcatcgagg agcggctgcg gcggcgccgt gcccagcgga tggcgcgcgc aaggctgggt
     7801 gtggagaaca ccgatgatcg tgagttcgat gtagtcccgt cgaggctgcg gcgcccgggt
     7861 tcagtggtgc tcgagatcga cgatgaggac cctttcttcg aacacctcga ctacgccccg
     7921 ttcgcacgcg acattgacct gccgcgagca gccggccaat aggattttcg gcggcgacgc
     7981 tgcggctggt agcctgctac cggcaccagg ggctatggcg cagttggtag cgcgactcgt
     8041 tcgcatcgag taggtcaggg gttcgattcc ccttagctcc acaacatttc agcaggtcag
     8101 ggcctgtttc taaagttacg tccgcaaccg cgtaacagtt cacgtaacat cgagggttgt
     8161 ggcaagcatc cacgcccaga agaaccgcgc cgggaacacc taccgggtgc tgtggcgcaa
     8221 aggcggccgc cagcgctcgc tgacatttga aaacttgtct gccgcagagc gtttcaagac
     8281 tctgctggag gatcacggac ccgatgaagc gctgcgaat
//
LOCUS       DASRJA010000629         9110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_36505,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000629 DASRJA010000000
VERSION     DASRJA010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9110
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36505"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VFC01_28210"
     CDS             complement(<1..545)
                     /locus_tag="VFC01_28210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83537.1"
                     /translation="MRPEGSAHIRADSATLLARFSERLRDEGVAVSPDQTMRLATLVA
                     LDVPRTVDELYWCARVSLLRSTADIAAFDSVFALVFRGMVDIAESRGDSTDPDDLRIP
                     PRHHDHVVNNELRAKAASTRRFEGAESTAGEEVGRPIPTLASWEEKLTKINFAQLTAA
                     EVRALAPLLRMLWVSPPLRLSR"
     gene            complement(535..1401)
                     /locus_tag="VFC01_28215"
     CDS             complement(535..1401)
                     /locus_tag="VFC01_28215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005512172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN83538.1"
                     /translation="MPDLETLRNALDGVDYLADDGTVMALFCASRLGQPVLIEGEPGV
                     GKTEAAKALAAALQTPLVRLQCYEGIDVAEALYEWNYPRQLLAIRLAEARGIELAEDA
                     LFSNGFLIARPLLLALRHPGPRPAVLLIDEVDRADDDFEAFLLELLGEGAVTIPELGT
                     VHATHRPIVVLTSNRTRELHDALKRRCLFHWIEYPTAVRAAEIVRRRVPGSSRTLAEQ
                     AARVVAQLRGLDLQKRPGIAETIDWVAALELLGVQAVDAATVDRTLGSVLKYVEDQQL
                     ARAAGLPADHAP"
     gene            complement(1431..2348)
                     /locus_tag="VFC01_28220"
     CDS             complement(1431..2348)
                     /locus_tag="VFC01_28220"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZN83539.1"
                     /translation="MPARLELAGRAEALAAAGTPFVWATVVRAERPTSAKPGDSALVL
                     ADGQVDGFVGGDCAESTVRAQALEVLATATPRLVRITPSPEDVGVADDDGVVTVHNPC
                     LSGGSLEIFLEPSVPLPVVAVFGHAPIATALLRAHRLFGQQVLRIDKPDDPLPSGARA
                     VVVASHGNDEEAVLEAALRAEVPYVALVASRRRGPAVLAALEVDDRLRSQVRTPAGLD
                     IGARTPPEVALSILAEIVAVLHEPGSASKDEPGPVHEMTELVRDPVCGMMVAPVDASP
                     HVETPNGVVYFCGTGCRNAYLDNPLAYAQ"
     gene            complement(2393..4771)
                     /locus_tag="VFC01_28225"
     CDS             complement(2393..4771)
                     /locus_tag="VFC01_28225"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HZN83540.1"
                     /translation="MTVTADQANVGFGRRMRKEDARFLRGKGNYVDDFVLPGMLHGAV
                     LRSPYAHARLVSIDTSAAEAHPKVRAVITGALLETLKLAWMPSLSYDVVAVLATDKVR
                     FHGQEVAFVVADDRYSARDALELIDVEYEPLPPVIDARRALDPDAAVIRDDIEGKTDN
                     HIFDWEVGDKAATDAAFADAEVVVSEDILYPRVHPAPLETCGAIADMDKVTGKLTIYC
                     NTQAPHAHRTLYAIVAGLPEHKIRILSPDIGGGFGNKVGIYPGYVLAVVGSILTGRPV
                     KWVEDRAENLMSTSFARDYHMHTEIAATREGKILATRVNVLADHGAFNNTAQPTKYPA
                     GFFHIFTGSYDYPAAHCTVKGVYTNKAPGGVAYACSFRITEAAYAIERIVDCLAFELD
                     MDPAELRMKNLLHAEQFPYKSPTGWEYDSGDYPRTLEMAMDMLGYDELRREQAEKRER
                     GELMGIGISFFTEGVGAGPRKHMDILGLGMADGAELRVHPTGKAVVRISVQSQGQGHE
                     TTFAQIVSEITGIPSDDIDVVHGDTDNTPFGLGTYGSRSTPVSGAAVAVAAKRVVDKA
                     KIIAAAMMEVSPDDIEIEKGTYSVKGDPTMSKTIQEVALAAHGSLELPEGVEGHLDAA
                     CVYDPPNLTYPYGAYICVVDVDPSTGAVKVRRFVAVDDCGTRINPMIVEGQIHGGLAD
                     GVGMALMEVIAFDESGNCLGGSFMDYQLPTSMECPSWELGETVTPSPHHPIGAKGVGE
                     SATVGSPAAVVNAVIDALKPYGVRHADMPLTPAQVWRTIQGRPFRTDLAITD"
     gene            complement(4768..5256)
                     /locus_tag="VFC01_28230"
     CDS             complement(4768..5256)
                     /locus_tag="VFC01_28230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZN83541.1"
                     /translation="MQVTVKVNGEEYSREIEPRLLLVHFLRDELGLTGTHWGCDTSNC
                     GVCVVQVDGTPVKSCTMLAAMASGHEVLTVEGLEQDGELDPIQQGFMTEHGLQCGFCT
                     PGMMLTTRWLLDANPHPSDAEIREAISGNLCRCTGYENIVRAVHWAADHPRVAETEEV
                     AS"
     gene            complement(5260..6135)
                     /locus_tag="VFC01_28235"
     CDS             complement(5260..6135)
                     /locus_tag="VFC01_28235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZN83542.1"
                     /translation="MQVPAPFEYERATSVDDAIALLERHGEEARLIAGGHSILPLMKL
                     RFAAPEVLIDINDLTELERIWVAGDQLCIGALVRHADLLASAVVGEHYRILHDAERVI
                     ADPVVRNRGTVGGSLCHADPAEDLSAALGAIRADVVIMGSGGERVVPVRGLQTGPFET
                     CIAPAEIATEIRLPIRPGTGSAYCKVERRAGDYAVAAAGAVVTLDGDTISEVGIGLAA
                     VGAPHFVASRAEDAIRGRGADDEAVSLAAQIAADECNPPTDQRGPSDYKRALAGELVK
                     RALRTAIARCRGQEA"
     gene            complement(6194..6490)
                     /locus_tag="VFC01_28240"
     CDS             complement(6194..6490)
                     /locus_tag="VFC01_28240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012690031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZN83543.1"
                     /translation="MSPKLGVLALLEAKPGKGDDLGKFLEGGRDLAAAEPGTVTWYAF
                     KITDTQYGIFDTFNDDDGRQAHLGGQIPAALSQVAEELLATAPDIRTVDVLAVK"
     gene            6580..7542
                     /locus_tag="VFC01_28245"
     CDS             6580..7542
                     /locus_tag="VFC01_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN83544.1"
                     /translation="MDIAVLAVDGCFDSGLSAVVDVLGTANALRQDVPAPPTGFTVTI
                     VGLKRQVRTGNGLTVNPVLAKKMAQHPDLLVMPALGVKTPQEIIDTVRGHAALDLIRD
                     NHANGTELAGACTGTFFLAESGVLDGRLATTSWWLGPAFRSRYPAVHVDETRIIARDA
                     QITTAGAAFAHIDLALSIVQQHSPSLADLTARYLLTGDRPSQAAFAMPALLARTDPTM
                     AAFEKWVRQRLSEPIRISAAARDLGISERTLQRTTASVVGMSPLHFIQQVRLDEATFL
                     LRDTPMSMSAIAAAVGYRDAGTLRALVRKRRNSTLRALRAGRPT"
     gene            complement(7539..8978)
                     /locus_tag="VFC01_28250"
     CDS             complement(7539..8978)
                     /locus_tag="VFC01_28250"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HZN83545.1"
                     /translation="MTGDDQDDISTAREAVVDARREAAAASARLAEAAVQYADCRTAD
                     DTAAGIGSGRRSRVRPGEFVADELALLLREQPYPMRCLLARTRRLASGLPTVWEAFRC
                     GDLDPEQIRIIDRVARRVTEPATLAAIDEHAVEAAQARSPKQLQVWLLRLVVQLEPLA
                     FQQRHRRALAERRVTVVQGADGIGYVTGEMSAADAAAIDAVLAAAARNLGADDPRTDQ
                     QRRADLFADLLLGRLAFDHTDTDDEDEEEEDTAGAESDWLEVENIDFDTGELLSTKLQ
                     RIDPQGQTIGEPVEAATHRPPGQPPKIIKRPQQQRIGIVVPLASLLDASDTPGELADR
                     SGFIPGDILIEQIAAALNSDSRDEVLFTRLLTDNGGRLLDVTEIGRYPSARLAQAIKI
                     RAGSCRFPTCTVPADRCDLDHHEPVPKGVTSGRNLDPFCRRHHRGKTFAWHAAHRDHD
                     GVDWTMPDAEHYRCIDEPLPTGRASVVRP"
BASE COUNT         1533 a         3035 c         2896 g         1646 t
ORIGIN      
        1 cgggacaacc gcagtggtgg gctgacccac agcatccgta gcaatggggc cagcgcacgg
       61 acctcggccg ctgtcagctg cgcaaagttg atcttggtca gcttctcttc ccaactcgcg
      121 agcgtcggta tcggccgtcc gacctcctcc cccgccgtgc tttccgctcc ctcgaatcgt
      181 cttgtggacg ccgccttcgc tctcagctcg ttgttgacaa cgtgatcatg gtgccgcggc
      241 ggtatccgaa gatcatcggg atcagtggaa tcgcctcgag actcggcaat atcgaccatc
      301 ccgcggaaca cgagtgcgaa gacggagtcg aatgcggcga tatcggccgt agatcgcagc
      361 aagctcaccc gggcgcacca gtacagttcg tccacggtcc tcggcacgtc gagtgccacc
      421 agcgtcgcga gccgcatcgt ctggtccggc gagacggcaa cgccctcgtc ccgaagccgc
      481 tcactgaatc gggccagcag cgtcgcactg tcggcacgga tgtgcgcgga gccctcaggg
      541 cgcatgatcg gccggcaacc cggcggcccg ggcgagctgc tggtcctcga cgtacttcag
      601 taccgagccg agtgtccggt cgactgtggc cgcgtccaca gcttgcacgc cgagcaactc
      661 cagcgcggcg acccagtcga ttgtttcggc gatgcctggc cgcttctgca aatccaaccc
      721 acgcagctgc gcgaccaccc gtgccgcctg ttcagcgagc gttcgcgagc tgccgggtac
      781 ccgccgccgg acgatttcgg ccgcccgcac cgcggttggg tactcgatcc agtgaaagag
      841 acatcggcgc ttgagcgcgt cgtgcagttc ccgagtccgg ttcgacgtca acacgacaat
      901 cggccgatgg gttgcgtgca ccgtgccgag ctcggggatg gtgacggctc cttcgccgag
      961 cagctccaga aggaacgcct cgaaatcgtc gtcggcgcga tcgacctcgt cgatcagaag
     1021 caccgccggt cgcgggccag gatggcgtag tgcgaggagc agcggacgcg cgatcaagaa
     1081 cccgttgctg aacagggcat cttcggccag ctcgatgccg cgcgcctcgg caagccggat
     1141 cgcgagcagc tgtcgaggat agttccactc gtagagcgcc tcggcgacgt cgatcccctc
     1201 atagcactgc agccgtacca gtggggtctg cagcgcggcg gcaagcgcct tcgcggcctc
     1261 ggtcttgccg acccctggct cgccctcgat aagaactggt tggcccagtc tggatgcaca
     1321 gaacagcgcc atcacggtgc catcgtcagc gaggtagtcg acaccgtcga gcgcatttcg
     1381 aagcgtctcc agatccggca gaagttccgc gacgctcgcg ttaccgccga tcactgggcg
     1441 tacgccaagg gattgtcgag gtaggcgttg cgacagccgg tgccgcagaa gtacaccacc
     1501 ccgttgggcg tctcgacgtg cggcgacgcg tcgacggggg cgaccatcat gccgcagacc
     1561 ggatcacgca ccagttcggt catctcgtgc acaggtccgg gttcatcctt gctagcagag
     1621 ccaggttcgt gcagcaccgc aacgatctcg gcgaggattg atagtgcgac ttcagggggt
     1681 gttcgcgcgc cgatatcgag tcctgccgga gtgcgtacct gcgatcgcaa ccgatcgtcc
     1741 acctccagcg cggcaaggac tgccggaccg cgtcgtcggc tggccaccag cgcgacatag
     1801 ggcacctccg ctcgtaatgc cgcctcaagg accgcttcct cgtcgttacc gtgcgaggcc
     1861 actaccaccg cacgagcccc cgacggcagg ggatcgtcgg gtttgtctat ccgcagaact
     1921 tgctggccga agaggcgatg ggcgcgcagc aaggctgtgg cgatcggagc gtgaccgaac
     1981 acagctacca ccggcaatgg aaccgacggc tccagaaaga tctccaggct gccgccagaa
     2041 aggcagggat tgtgtaccgt caccacgcca tcatcgtcgg cgacaccgac atcctcgggc
     2101 gacggcgtga tgcgcaccaa ccgtggcgtg gcggtagcca gcacctccag tgcctgagcc
     2161 cgcaccgtcg actccgcaca gtcgccgccg acgaagccgt caacttgacc gtccgcgagc
     2221 accagcgccg aatcgcccgg ttttgcgctg gtcggtcgtt ctgcccggac gaccgtcgcc
     2281 catacgaagg gggtgccggc agcggcgagc gcctcagccc gtcctgcgag ctctagacgc
     2341 gccggcatcg gcccctcctc aaaccaggcc ccacctcaag atcagcaccg cctcagtcgg
     2401 tgatcgccag gtcggtacgg aacggccggc cctggatcgt ccgccacacc tgggctggtg
     2461 tcagtggcat gtcggcgtga cgtacgccgt agggcttcag ggcgtcgatc accgcattga
     2521 ccacagcagc cggcgagccg acggtggccg actcaccgac acctttcgct cctatcggat
     2581 ggtgcggcga gggggtcacg gtctctccga gctcccagct ggggcactcc atcgaggtgg
     2641 gcagctggta gtccatgaac gaccctccga ggcagttccc gctctcgtcg aacgcgatga
     2701 cctccatcag cgccatgccg acaccgtcgg cgagcccacc gtggatctgc ccttccacga
     2761 tcatcggatt gattcgggtg ccgcagtcgt caactgcgac gaaccgccgc accttcacgg
     2821 ctccagtgct cggatccacg tcaacgacgc agatgtaggc cccatacgga taggtcaagt
     2881 tgggtgggtc gtacacgcac gccgcatcca gatgcccctc gacaccctcg ggcaactcca
     2941 gtgatccgtg cgccgcaagc gctacctcct ggatcgtctt cgacatcgtc gggtcaccct
     3001 tgaccgagta ggtgcctttc tcgatttcga tatcgtccgg cgagacctcc atcatcgccg
     3061 ccgcgatgat cttcgctttg tcgacgacac gcttcgccgc gaccgcaacc gcggcgcccg
     3121 agaccggcgt ggaacggctg ccgtaggtgc ctagcccgaa aggagtgttg tcggtatcgc
     3181 cgtggacgac atcgatgtcg tcggacggta ttcccgtgat ctcggagacg atctgggcaa
     3241 aagtcgtctc gtgtccttga ccctgcgact ggaccgagat tcgtaccacg gccttgcccg
     3301 tgggatggac ccgcaactcg gcaccgtcgg ccatcccgag gcccaggatg tccatgtgct
     3361 tgcgcggccc agcgccgaca ccctcggtga agaagctgat gccgattccc atcagctcgc
     3421 cgcgctcgcg cttctcggcc tgttcccggc gcagctcgtc gtagccgagc atgtccatcg
     3481 ccatctccag cgtgcgcggg tagtcgcccg agtcgtactc ccagccggtc ggcgatttgt
     3541 acgggaattg ctccgcatgg agcaggttct tcatgcgcag ctccgcaggg tccatgtcca
     3601 attcaaaggc caggcagtcg acgatgcgct cgatggcgta cgccgcctcg gtgatccgga
     3661 acgaacacgc gtacgccacg ccgccggggg ccttgttggt gtagacgccc ttcacggtgc
     3721 agtgcgcggc ggggtagtcg taactgcccg tgaagatatg gaaaaagcca gctggatact
     3781 ttgtcggctg cgcggtgttg ttgaacgcgc catgatcagc caggacgttc accctcgttg
     3841 ccaagatctt gccttcgcgc gtcgctgcga tttcggtgtg catgtgatag tcgcgcgcga
     3901 aggaggtcga catcaggttc tcggcacgat cctcgaccca cttgacgggc cggccggtga
     3961 ggatcgaccc gaccacggcc aggacatagc ccggatagat gccgaccttg ttcccgaatc
     4021 cgcccccgat gtcgggactg aggatcctga tcttgtgctc gggaaggccg gcaacgatgg
     4081 cgtacagcgt gcggtgtgcg tgcggagcct gcgtattgca gtagatcgtg agcttgccgg
     4141 tgaccttgtc catgtcggca atggcaccgc aggtctcgag tggggcggga tgcacgcgcg
     4201 ggtaaaggat gtcctccgag accaccacct ccgcatcagc gaacgcggcg tccgtcgccg
     4261 ccttgtcgcc gacctcccag tcgaagatgt ggttatcggt cttgccttcg atgtcgtcgc
     4321 gaatgaccgc cgcatcggga tcgagggctc ggcgcgcgtc gatgaccggg ggcagcggct
     4381 cgtattcgac gtcgatcagc tccagcgcgt cccgtgccga atagcggtcg tcggcgacaa
     4441 cgaaggccac ctcctgccca tggaagcgca ccttgtcggt ggcgagcacg gctaccacgt
     4501 catacgacag cgacggcatc caggcaagct tcagggtctc cagcagtgcc ccagtgatca
     4561 ctgcgcggac tttcggatgc gcctcggctg ctgaggtgtc gatcgagacc agccgtgcat
     4621 gcgcgtacgg gctgcgcagc accgcgccgt gcagcatccc cggcagcacg aagtcgtcga
     4681 cgtagttgcc cttgccgcgc aggaatcgag cgtcctcctt gcgcatccgg cgcccgaaac
     4741 caacattcgc ctggtccgcg gtgacggtca tgacgcgacc tcctcagttt cggcaacccg
     4801 cgggtggtct gcggcccagt ggaccgcgcg cacgatgttc tcgtatcccg tgcagcgaca
     4861 cagattgccc gagatcgctt cgcggatctc ggcgtccgac gggtgcgggt tggcgtcgag
     4921 cagccatcgg gtggtcaaca tcattcctgg ggtacagaat ccgcactgca gaccgtgctc
     4981 ggtcataaac ccctgctgga tgggatcgag ctcgccgtct tgttccaggc cctcgaccgt
     5041 cagaacttcg tgcccactag ccatcgccgc gagcatggtg caactcttca ccggggtgcc
     5101 gtcgacctgg accacgcaga cgccgcagtt ggacgtatcg cagccccagt gggtgccggt
     5161 caggccgagc tcgtcgcgca ggaagtgcac cagcagcagc cgcggctcga tctcgcggct
     5221 gtactcctcg ccattcacct tgactgtgac ctgcatgatt catgcctcct gtccgcggca
     5281 acgggcgatc gcggttcgca atgcccgctt cacgagttcg ccggcgagcg cgcgtttgta
     5341 gtccgaaggc ccacgctggt cggtgggcgg gttacactcg tccgccgcga tttgggcggc
     5401 caggctgact gcctcatcgt ccgcaccgcg gccccgtatg gcgtcctcgg cccgggaggc
     5461 aacgaaatgc ggcgccccta ccgcggcgag accgatgcca acctcgctga tcgtgtcacc
     5521 gtccaaggtg acgaccgcac ccgccgcggc taccgcatag tccccggctc gccgttccac
     5581 cttgcagtac gcgcttcctg tgccgggccg gatcggcagc cgaatctcgg tggcaatctc
     5641 agccggggcg atgcacgtct caaacggtcc ggtctgaagc ccgcgtactg gcaccacccg
     5701 ctcgccaccc gagcccatga tcacgacgtc cgcgcggatc gcaccgagcg cggcggaaag
     5761 atcttcggca gggtcagcgt ggcacagcga cccgccgaca gtgcctcggt ttcggaccac
     5821 agggtcggcg atcacccgct ccgcgtcgtg aaggatccgg tagtgctcac cgaccaccgc
     5881 cgaagcgagc aagtcggcat ggcggaccag cgcaccgata cacaactggt cacctgctac
     5941 ccagatgcgc tccagctccg tcaggtcatt gatatcgatg agaacttcag gcgcagcaaa
     6001 acgcagcttc atcagcggca atatgctgtg ccctccggcg atcagccgtg cctcctcacc
     6061 atgccgctcc agcagtgcga tggcgtcgtc gacgctggtg gcgcgctcgt actcgaacgg
     6121 agccggtacc tgcattgatc cctcctcagt aggtctctcg atagggatgg cgttgctgca
     6181 cgatcggcgc cgtttacttc actgccaaca catcgaccgt gcggatgtcg ggtgcagtag
     6241 cgagcaattc ctcggctacc tggctcagtg ctgccgggat ctggccgccc aggtgtgctt
     6301 gacggccatc gtcgtcattg aaggtgtcga agatcccgta ctgggtgtcg gtgatcttga
     6361 acgcgtacca ggtcaccgtg cccggttcgg ctgcggcgag gtcgcgtccg ccctctagga
     6421 acttcccaag atcgtcaccc ttgccaggct tggcttcgag cagcgcaagt actcccaact
     6481 tcggactcat cggaactcct cccaggatgt ccctaccgta cgtatccaaa cggtccaacc
     6541 ggtagtggcg gacccgccac gttgcgtatc gtttccgacg tggacatagc ggttctcgcg
     6601 gtggacggct gcttcgactc agggctttcc gcggtggtcg acgtgctcgg gacggcgaac
     6661 gccctccgac aggatgtgcc ggcacccccg accgggttca cggtgacgat cgttggactc
     6721 aaaaggcagg tgcggacggg caacggcttg actgtgaatc ccgttcttgc caagaagatg
     6781 gcgcagcatc cggatctgct ggtgatgccg gcactgggcg tcaagacacc tcaggagatc
     6841 atcgacaccg tccgcgggca cgcggccctc gacctcatcc gggataacca cgcgaacggc
     6901 acggagctgg ctggtgcctg cacgggaacc ttcttcttgg ccgagtctgg tgtccttgac
     6961 ggacggctgg ccacgaccag ctggtggctc ggacccgcgt tccgcagccg gtatccagcg
     7021 gtacacgtgg acgagactcg gatcatcgct cgggatgcgc agattaccac cgccggtgcc
     7081 gcattcgccc atattgatct tgcactgtcg atcgtgcagc agcacagccc gagcttggca
     7141 gacctcaccg cgcggtatct gctcaccggc gaccggccgt cgcaggcagc gttcgccatg
     7201 ccggcgctgc ttgcccggac cgacccgacg atggcagcgt tcgaaaagtg ggttcggcaa
     7261 cgactttccg aacccatccg catatcggcc gctgcgcgcg acctcggtat cagcgagcgg
     7321 acgctgcagc gtacgaccgc ctccgtcgtc ggcatgtcgc cactgcactt catccagcag
     7381 gtacgcctcg acgaggccac cttcttgctg cgcgatacgc ccatgtcgat gagcgcgatc
     7441 gcggcggcgg tcggctaccg ggatgccgga actctccggg ccctcgttcg caagcgtcgc
     7501 aacagcacgc tgagagcgtt gcgggcaggc cgaccgacct aagggcgaac gaccgacgct
     7561 cgtccagtgg gtagtggttc gtcgatgcat cggtagtgtt cggcgtcggg catggtccag
     7621 tcgacgccgt cgtggtctcg gtgtgcggcg tgccaggcga aggtcttacc gcgatggtgt
     7681 cggcggcaga acgggtcaag atttcgtccc gacgtaacgc ctttgggcac tggttcgtgg
     7741 tggtcgaggt cacagcggtc ggcaggcact gtgcaggtgg ggaatcggca gctgccggcc
     7801 cgaatcttga tcgcttgggc caaccgggct gaggggtagc gaccgatttc ggtgacatcg
     7861 agcagccgac cgccattgtc ggtgagcagc cgggtgaaca agacttcgtc gcggctgtcc
     7921 gaattgaggg ctgcagcgat ttgttctatg aggatgtcgc cggggatgaa accggatcgg
     7981 tcggctagtt cgccgggtgt gtcgctggca tcgagcaggc tggccagcgg caccactatg
     8041 ccgatgcgtt gttgctgcgg tcgtttgatg atctttggtg gctgaccggg gggacggtgt
     8101 gtcgctgcct caaccggctc gccaatggtt tgaccttgag gatcgatgcg ttgcagcttg
     8161 gtgctgagga gttcgccggt gtcgaaatcg atgttctcca cttccagcca gtcggattcg
     8221 gcaccggcag tgtcttcttc ctcctcatcc tcgtcgtcgg tgtcggtgtg gtcgaaagcg
     8281 agccggccga gcagcaggtc ggcgaacagg tcggcgcggc gttgctggtc ggtgcgtggg
     8341 tcgtcggcgc cgaggttccg ggcggcagcc gccagcaccg catcgatcgc cgcggcgtcg
     8401 gccgccgaca tttcgccggt gacataaccg atgccgtcgg caccttgcac cacagtgacc
     8461 cgacgttcgg cgagggcccg gcgatgccgc tgctggaacg ccaacggttc cagttgcact
     8521 accagccgca gcagccacac ctgaagctgc ttcggagacc tagcctgtgc cgcctcgacg
     8581 gcgtgttcgt cgatggcggc caatgtggcg ggctcggtga cccgccgcgc cacccgatcg
     8641 atgatccgga tctgctcggg gtccagatcg ccgcaccgga acgcttccca cacggtgggc
     8701 aggccgctgg cgaggcggcg agtacgggcc agcagacagc gcatggggta gggctgctcg
     8761 cgcagcagca gtgccaactc atccgcgacg aactctcccg gtctgacccg actcctccga
     8821 cccgatccaa tgccggctgc ggtgtcgtcg gcagtccgac aatcggcgta ctggaccgct
     8881 gcttcggcca accgggccga cgcggcggcg gcctctcggc gcgcatcgac cactgcctct
     8941 cgggcggttg atatgtcgtc ttgatcgtcc cccgtcatgg attcatcgtc gcaaccgcca
     9001 ctgacagttt tcgaacatac gagcgacacg ctgagagatc gatcaccgat catgggatcg
     9061 cgattggtca caaccgcatc gacaccgtca tcgcggtgcg ctcggtggtc
//
LOCUS       DASRJA010000630         4082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3702797,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000630 DASRJA010000000
VERSION     DASRJA010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3702797"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFC01_28255"
     CDS             complement(<1..204)
                     /locus_tag="VFC01_28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride ion channel protein"
                     /protein_id="HZN83546.1"
                     /translation="MTRRTLEFGCAIVIIGLLAGVAGAATTLLLHAVEHLTYHYTFGT
                     LLTGVGESSPVRRAIGPMVGGALA"
     gene            235..1119
                     /gene="mshB"
                     /locus_tag="VFC01_28260"
     CDS             235..1119
                     /gene="mshB"
                     /locus_tag="VFC01_28260"
                     /EC_number="3.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-
                     D-glucopyranoside deacetylase"
                     /protein_id="HZN83547.1"
                     /translation="MVQMETPRLLFVHAHPDDETLTTGATIAHYVSRGAQVRVVTCTL
                     GEEGEVIGERWAQLAVDHADQLGGYRIAELSAALNALGVDGPIFLGGAGRWRDSGMEG
                     TAPRHRQRFVDADPREAVGALVSVIRDLRPHVVVTYDANGGYGHPDHIHAHDVTVAAV
                     AASRSNEYPGEPWTVPKLYWTVIAKSAMGAGLDALDDVPPEWIRVSVDDVPFGYPDEA
                     INAVMDVPDQLPAKVAALRAHATQVTVAPDGRSCALSNDIALPIGAIEHYILAAGTPG
                     ERDARGWETDLLAGLNLE"
     gene            1233..1646
                     /locus_tag="VFC01_28265"
     CDS             1233..1646
                     /locus_tag="VFC01_28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83548.1"
                     /translation="MGARVARGAARQHPDLTPPRPAVLTLLAIDGVLSAVAAAVFLPL
                     RIGSVPFPISALISGLVNAALVWAALQWTSAPRVAALPLWCWLLTVAGLTFGGPGDDI
                     VFGGVGVMEYAPLLLLVLGALPPALVLWRYVRSAV"
     gene            complement(1650..2684)
                     /locus_tag="VFC01_28270"
     CDS             complement(1650..2684)
                     /locus_tag="VFC01_28270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83549.1"
                     /translation="MGHLIRSCLTVGVATVTAAAVAVCPSVQEPVSSPTRASTVRLIA
                     AVQPLALAEQPPLLNVLLSSPLRLLGPAATPGTLPPPPAPIQFAIAPNLANTIDGVYT
                     AVEPWVRYGFEVATAVVRWIPYVGWFAGQIMVFYNFFESMVASGVFNFTDWLRGDGGV
                     VENLVDFGVDVGLAFVWLGLDEVAQFIPLPPFCCYPPRPPVQGPFLALDTLAGPAETA
                     DAMMAASPADISQPTEKRSAVKGEDLAEAPDEVATDPADEVAVSGEREPKIDAVKEPK
                     EPKAKADVAAQGEVRAVSTESTSESTTVSTGGKKADNGPAAKGATESSSSPTNDENKG
                     EKKDDTGKKE"
     gene            2890..>4082
                     /gene="cofG"
                     /locus_tag="VFC01_28275"
     CDS             2890..>4082
                     /gene="cofG"
                     /locus_tag="VFC01_28275"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HZN83550.1"
                     /translation="MALNPQRGADLPSPVVPVKSAAPSSAALRRVLRRARDGVALNVD
                     EAAIAMTARGEDLADLCASAARVRDAGLESAGRRGANGRLPVSYSRKVFIPVTRLCRD
                     TCHYCTFVTVPGKLRAAGMGMYMEPDEILDVARRGAELGCKEALFTLGDRPEARWDEA
                     RQWLDERGYDSTLDYVRAMAIRVLEETGLLPHLNPGVMSWSELSRLKPVAPSMGMMLE
                     TTSRRLFETKGLAHYGSPDKDPEVRLRTLADAGRLSIPFTTGLLVGIGETLVERAETI
                     HAIRQSHKEFGHVQEVIVQNFRAKDHTAMASVPDAGFDDFVATVAVTRLVMGPKMRIQ
                     APPNLVSRAECMALLGAGVDDWGGVSPLTPDHVNPERPWPALDELEAVTAEAGYDLVQ
                     RLTAQP"
BASE COUNT          636 a         1332 c         1417 g          697 t
ORIGIN      
        1 ggcgagtgca ccaccgacca tcggcccgat ggcgcgccgg accgggctgc tttcgccgac
       61 gccggtcaac agcgtgccga atgtgtagtg atacgtgagg tgttcgaccg cgtgcagcag
      121 cagggtcgtg gcggcaccgg cgacgccagc cagcaggccg atgatgacaa tcgcacaccc
      181 gaactcaagg gtgcgacgcg tcaccgggtg aatctatgtc agcgaccggg caagctggtg
      241 caaatggaga ccccgcggtt gctgttcgtg cacgcccatc ccgacgacga gaccctcacg
      301 acgggcgcga ccatcgccca ctacgtctcc cgcggcgcgc aggtgcgtgt cgtcacgtgc
      361 acgctcggcg aggaggggga ggtgatcggc gagcgctggg cccagctggc ggtcgaccac
      421 gctgatcagc tgggcggtta ccgcattgcc gagctgagcg ctgccctgaa cgcgctggga
      481 gtcgacggac cgatcttcct cggcggcgcc gggcggtggc gcgactcggg gatggagggc
      541 acggcgccgc gacaccgtca gcgattcgtc gacgccgatc cgcgcgaggc cgtcggcgcg
      601 ttggtgagcg tcatccgcga tctgcgcccg cacgtcgtcg tcacctacga cgccaacggc
      661 ggctacggcc atcccgacca catacacgcg cacgacgtca cggtggccgc tgtggccgca
      721 tcgagaagca atgagtatcc cggcgaacca tggacggtgc cgaagctgta ctggaccgtg
      781 attgcgaagt ctgcgatggg cgcgggtctc gacgcgctcg acgacgtgcc gccggagtgg
      841 atcagggtct cggtcgacga cgtcccgttc ggctaccccg acgaggcgat caacgcggtg
      901 atggacgtgc ccgaccagct gccagcgaag gtcgcagcct tgcgtgcgca cgcgacccag
      961 gtgaccgtgg ccccggacgg ccggtcctgc gccctgtcga acgatatcgc gctgccgatc
     1021 ggcgcaatcg agcactacat ccttgcggcg ggcacgcccg gggagcgcga cgcccgcggg
     1081 tgggaaaccg acctgcttgc cggcctgaac ctggaatagc gcaacgggct cgcggtaagc
     1141 tgcccgaaat cggtcgcgtg tggagggatg gcaatggacc cggacatgga tcccaacctg
     1201 cagcactggc aggaccgcgc agacaacttc cagtgggtgc tcgggtcgct cgtggcgctg
     1261 ctcgacagca tcccgacctg accccgccgc gtcccgccgt tctgacgctg ctggcgatcg
     1321 acggcgtgct ctcggccgtg gcggccgcgg ttttccttcc gttgcgaatt ggctcggtgc
     1381 cgtttccgat cagcgctctg atcagtgggc tcgtcaacgc ggcactggtg tgggcggcgc
     1441 ttcagtggac gtcggctccg cgggtcgcgg cgttgccgtt gtggtgctgg ctgctgaccg
     1501 tcgccgggct gacattcggc gggccgggcg acgacatcgt gttcggcggt gtcggggtga
     1561 tggagtacgc cccgctgctg ctgcttgtcc tgggcgcgct gccccctgcg ctggtgttgt
     1621 ggcgctacgt gcggtcggcc gtatgacggc tactccttct tgcctgtgtc gtccttcttc
     1681 tcgcctttgt tttcgtcatt ggtcggcgag ctcgacgatt ccgtcgcacc cttggccgcc
     1741 ggtccgttat cggctttctt cccgccggtt gagaccgtcg tagattccga agtgctctcc
     1801 gttgacacgg cgcggacctc accctgagcc gcgacgtcgg ccttcgcctt gggttcctta
     1861 ggctccttga ccgcgtcgat cttcggttcc ctttcgccac tgaccgcaac ctcgtcggcg
     1921 gggtcggtcg caacttcatc gggtgcctca gcgaggtcct cacctttcac cgcactgcgc
     1981 ttttcggtgg gctgcgagat gtcggcaggc gatgcagcca tcatcgcgtc ggccgtctca
     2041 gccgggcccg caagcgtgtc gagggcaagg aacggacctt gcactggagg cctcggcggg
     2101 tagcagcaga acggcggcaa cgggatgaac tgagccactt catcgagtcc cagccagacg
     2161 aatgccaggc cgacatcgac gccgaaatcg accagattct cgacgacgcc gccgtcgccg
     2221 cggagccaat cggtgaagtt gaaaacgccg ctggcgacca tgctttcgaa gaagttgtag
     2281 aagaccatga tctgaccggc gaaccagccg acatacggaa tccagcgcac caccgcggtg
     2341 gcgacctcga acccatatcg cacccacggc tcaaccgcgg tgtagacgcc gtcgatcgtg
     2401 ttcgcgagat tcggtgcaat cgcgaactga atcggcgccg ggggcggggg caatgtgccc
     2461 ggtgtcgctg cgggccccag cagccgcagc ggcgagctga gcaggacatt gagtagtggc
     2521 ggttgttccg ctagtgccag tggctgtacg gcggcgatca gtcggaccgt cgaggcccgt
     2581 gttggtgacg acacgggttc ctggacggat gggcagacgg cgacggccgc tgcggtgacg
     2641 gtcgcgacac ctaccgtcag gcatgaacga atcaaatgac ccatgagaac ccctcccgcg
     2701 agctgcggtc ctccccggct gtgaacttac gcggaggtaa aagctgtcgt gccacattcg
     2761 gcaacacaag ccaggacggg cccacaccgc agtggcgggc tcggactact gtggccgata
     2821 tgctcggcgc agtggtttca gaatgtgaga cgcgcggatg attcttgacg accgtggggt
     2881 taaaccaggg tggctctgaa cccccagcgc ggcgccgatc tgcccagccc ggtggtccct
     2941 gtgaagtccg ctgcccccag ctctgccgct ttgaggcggg tgttgcgccg ggcccgcgac
     3001 ggcgtcgcac tcaacgtcga cgaggccgcg atcgccatga ccgcccgtgg cgaggatctg
     3061 gctgatttgt gcgcgagtgc cgcgcgagtg cgcgacgccg ggctcgagtc cgccggacgc
     3121 cgtggggcca acggccggct gcccgtctcc tactcgcgca aggtgttcat ccccgtcact
     3181 cgtctgtgcc gcgacacctg ccactactgc acgttcgtca cggtgccggg caagctgcgg
     3241 gccgcgggga tgggcatgta catggagccc gacgagattc tcgacgtggc ccgccggggt
     3301 gccgaattgg gttgcaagga ggcgttgttc acactcggtg atcggccgga ggcgcggtgg
     3361 gacgaggccc ggcagtggct cgacgagcgc ggctatgact cgacgctgga ctacgtgcgc
     3421 gcgatggcga tccgggtgct cgaggagacc gggctgctgc cccacctgaa cccgggcgtc
     3481 atgtcgtggt cggagctctc gcggctcaag ccggtcgcgc cgtccatggg gatgatgctg
     3541 gagacgacgt cgcggcggct gttcgaaacc aagggcctgg cgcactacgg cagccccgac
     3601 aaggatcccg aggtgaggct gcgcaccctg gccgatgccg gcaggttgtc gattccgttc
     3661 accaccggac tgctggtcgg catcggggag acgctcgtcg agcgtgccga gaccatccat
     3721 gcaattcgcc agtcgcacaa ggaattcggg cacgttcagg aagtgatcgt gcagaacttc
     3781 agggccaagg accacaccgc catggcctcg gttcccgacg cgggcttcga tgatttcgtc
     3841 gcgacggtgg ccgtcacccg gctggtgatg ggtcccaaga tgcgcattca ggcgccgccg
     3901 aacctggtgt cgcgcgccga gtgcatggcg ttgctcggcg ccggcgtcga cgactggggt
     3961 ggggtgtcgc cgctgacgcc ggatcacgtc aaccccgagc gcccgtggcc cgcgctggac
     4021 gagctcgaag cggtcaccgc cgaggcgggc tacgacttgg tgcagcggct gaccgctcag
     4081 cc
//
LOCUS       DASRJA010000631        10871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_641146,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000631 DASRJA010000000
VERSION     DASRJA010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10871
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641146"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VFC01_28280"
     CDS             complement(<1..1241)
                     /locus_tag="VFC01_28280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HZN83551.1"
                     /translation="MSRFTEKMYRNARTVTTGMVTGEPHEPVRHTWGEVHERARCIAG
                     GLAAAGIGHGDAVGVLAGFPVEIAPTAQGLWMRGASLTMLHQPTPRTDLVIWAEDTMT
                     VIGMIEAKAVIVSEPFTAAIPVLEEKGIKVLQIADLLTSDPIDPVDVGEDDLALMQLT
                     SGSTGSPKAVQITHRNIHSNAEAMFIGAEYDVDKDVMVSWLPCFHDMGMVGFLTIPMY
                     FGAELVKVTPMDFLRDTLLWAKLIDKYKGTMTAAPNFAYALFAKRLRKQAEPGQFDLS
                     TLRFALSGAEPVEPADVEDLLDAGKPFGLRPDAILPAYGMAETTLAVSFSPCNAGLVV
                     DEVDADLLAALRRAVPATKGNTRRLASLGPLLKDLEARIIDEHGNVMPPRGVGVIELR
                     GESLTPGYITMGGFIPAQDEHG"
     gene            complement(1520..2485)
                     /locus_tag="VFC01_28285"
     CDS             complement(1520..2485)
                     /locus_tag="VFC01_28285"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZN83552.1"
                     /translation="MSASDGSTASEWVPTGSVTVRVPGKVNLYLGVGDRRDDGYHELT
                     TIFHAVSLLDEVIVRNADVLSLEMAGEGVESLPTDERNLAWRAAELMAEHVGRSPDVA
                     ISIEKSIPVAGGMAGGSADAAAVLVAMNTLWELGVPRRDLHALAAQLGSDVPFALHGG
                     TALGTGRGEELATVLARSTFHWVLAFAEGGLSTPAVFSEIDRLRDKGRGQPPRLDDPE
                     PVLAALASGDPAELAPLLGNDLQPAALSLDPGLRRTLRAGADAGALAGIVSGSGPTCA
                     FLCMSAAAAVDVGAELAGAGVCRTVRVASGPVHGARVVPAPSTSV"
     gene            2597..3394
                     /locus_tag="VFC01_28290"
     CDS             2597..3394
                     /locus_tag="VFC01_28290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN83553.1"
                     /translation="MLSPGDRFEGYVVEAVLGHGGSATVYQAHDSAGPDRAVALKVLD
                     ERHRHQAQLERLRREFEFAHQLDHAHIVRMYERGPGWLSMELVPGGTAAKLSTLPDRL
                     AALSQIADALDYIHNRGIVHCDVKPTNILVHPDFSQNGAVLIDFGVALSITSDIGYHA
                     THIEASLPYSAPELLTGHAPSEATDEYALACTAVELITGSPPFTATTQLGLIKAHLSS
                     TPPRRSREITWLPRVFDSILAKAMSKSPQNRYQSCREFVSLITRALR"
     gene            3476..3835
                     /locus_tag="VFC01_28295"
                     /pseudo
     CDS             3476..3835
                     /locus_tag="VFC01_28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019666610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(3958..5160)
                     /locus_tag="VFC01_28300"
     CDS             complement(3958..5160)
                     /locus_tag="VFC01_28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZN83554.1"
                     /translation="MGRGGIVRRAYVFRLRPTARQHAALAACVDAHRELYNAALQERR
                     DAWSHSRTRIYYGDQSAQLTAIRAARPDMDVWSFSSQQATLRRLNKAFAGFFRRVKTA
                     KPGVKPGFPRFKGKARFDSVEWPKDGDGARWLREQKRVYLQGIGQVKVHLHRQVVGRV
                     KTIQVKRCGRHWLLVLSCEDVPTNPLPATGRQAGIDVGIASFATTSEGECVDNPRWGR
                     AADDRLTAAQQRLQCAKRRSKNRDRKRETVAARHRKIANQRKDFHHKQARQLVARFDL
                     LVVEDLKIANMLRRAKVPDPDNPKQFLKNGARAKSGLNRSISDAGWGRFVSILRAKAE
                     EAGRIWIEVDPRHTSDGCEKCGYAAAENRVTQAEFVCQRCSHRAQADEHAARNILRAG
                     LALRAQAA"
     gene            complement(5139..5548)
                     /gene="tnpA"
                     /locus_tag="VFC01_28305"
                     /pseudo
     CDS             complement(5139..5548)
                     /gene="tnpA"
                     /locus_tag="VFC01_28305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5652..6596)
                     /gene="rsmA"
                     /locus_tag="VFC01_28310"
     CDS             complement(5652..6596)
                     /gene="rsmA"
                     /locus_tag="VFC01_28310"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZN83555.1"
                     /translation="MTIRLLGRTEIRHLAKEIDFRPRKSFGQNFVHDANTVRRIVSAS
                     GVNRHDHVLEVGPGLGSLTLALLDRGARVTAVEIDPILARQLPKTIAEHSHSEINRLA
                     VLNRDILTLHPPDVEEQPTALVANLPYNVAVPALLHLLAEFPTIRTVMVMVQAEVAER
                     LAAEPGGKEYGVPSVKVRFFGNVRRYGMVSPTVFWPIPRVYSGLVRIDRHEASPWPTD
                     ADFRTKVFELIDVAFSQRRKTSRNAFAEWAGSGNESANRLLAASIDPARRGETLSVAD
                     FVRLLQRSGEWEPKPTEPQEPQPQPQEQPAQQSQIRAI"
     gene            complement(6593..7720)
                     /locus_tag="VFC01_28315"
     CDS             complement(6593..7720)
                     /locus_tag="VFC01_28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HZN83556.1"
                     /translation="MNTLNKLHEARSPLLRLLVGATLLALTFAGGYAVAAHKTVKLTV
                     DGSEMTVATMKSRVIDVVKENGFDVGDRDDLYPAANEQVRQSDSIVLRRSRPLQISLD
                     GENSRQVWTTASTVDEALAQLKMTDKAPAAASRGSRVPLAGMSLPVVSAKTVQLNDAG
                     ATRTIHLAAPNVAGLLEAAGVPLKQNDTVVPPAWSPVVDNMQVQVTRIRIDKVTQRLP
                     LPPNNQQIPDATMNMSRQVVDDPGMPGTQDVTFAVSTVNGVETGRLPVANVVIAPARD
                     GVLRVGAKPGTEVPPVTNGAVWDSLSKCEAGGNWAINTGNGFYGGVQFDQNTWERQGG
                     LRYAPRADLATREEQIAIAEVTRARQGWGAWPVCSGRTGAR"
     gene            complement(7797..8645)
                     /locus_tag="VFC01_28320"
     CDS             complement(7797..8645)
                     /locus_tag="VFC01_28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZN83557.1"
                     /translation="MDQKRPAPPAPEPLSPLIDAHTHLDACGATDAASVTAIVDRAAA
                     VGVQAVVTIADDLDSARWVTEAADWDDRVYAAVALHPTRANALTDEAKTVIERLAEHP
                     RVVAVGETGMDMYWPGKLDGCAEPAAQKDAFAWHIDLAKRIGKPLMIHNRDADAQVLD
                     VLQAEGAPDTVIFHCFSSDAAMARTCVASGWLLSLSGTVSFKNAKALREAAALIPPDQ
                     LLVETDAPFLTPHPYRGAPNEPYCLPYTVRALAEVVDRPAEDIAQASTTAAIRAYGLA
                     SCRPLT"
     gene            8670..10205
                     /gene="metG"
                     /locus_tag="VFC01_28325"
     CDS             8670..10205
                     /gene="metG"
                     /locus_tag="VFC01_28325"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZN83558.1"
                     /translation="MSEPYYITTAIAYPNGDPHVGHAYEYIATDAIARFKRLDGFDVR
                     FLTGTDVHGQKMAETAAAEGIPAAELARRNSDVFQRMQERLNISFDRFIRTSDADHYE
                     ASKEIWRRMNEAGDIYLDAYKGWYSIRDERFFTEAETTVGADGVRIATETGAPVTWTE
                     EQTYFFKLSAYADKLLALYKAHPEFIEPDVRRNEVVSFVSGGLRDLSISRTTFDWGVP
                     VPDHPDHVMYVWVDALTNYLTGVGFPDTSSDLFKRFWPADLHMIGKDIIRFHTVYWPA
                     FLMSAGIELPRKVFVHGFLLNKGEKMSKSVGNVVDPLNLIDTFGVDQVRYFFLREVPF
                     GQDGSYSEEAIIGRINADLANELGNLAQRSLSMVAKNLDGIVPEPSEFTADDKALLEA
                     ADALMDRVRSHYDATAMHLALESIWSVLGAANKYFSAQEPWVLRKTDEERFRTVLYTT
                     LETVRIATLLSQPVMPESTGKLLDLLGQTDDQRTFAAIGSRLAPGTALPAPVGVFPRY
                     QAE"
     gene            10283..>10871
                     /locus_tag="VFC01_28330"
     CDS             10283..>10871
                     /locus_tag="VFC01_28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN83559.1"
                     /translation="MTDMFHLGWFLNFVADEWNGNWGDGGRDFTGDFYVEIARDLERG
                     RFDYVLLEDKLMVSTAYGGTMEHDLKHGVNPKHDPVPLAVLMANATSRLGVIPTMSTT
                     FYPPFLLARLCSTIDHIARGRFGWNVVTSGEDRSAQNFGMDKLYEHDDRYARASEYME
                     VVTKLWESWEPDAIERNTTTGTFANFKKVHTIDFDG"
BASE COUNT         1873 a         3605 c         3493 g         1900 t
ORIGIN      
        1 ccgtgctcgt cctgcgcggg gatgaagccg cccatggtga tgtaaccggg cgtcagcgac
       61 tcaccgcgta gctcgatcac gccgacgccc cgcggcggca tcacattgcc gtgctcgtcg
      121 atgatgcggg cctcgaggtc cttcagtagc gggcccaacg aggccagccg gcgagtgttg
      181 cccttcgtgg cggggacggc gcgacgcagc gcggccagca ggtcggcgtc gacctcgtcg
      241 accaccagac cggcgttgca cggggagaac gacaccgcca gcgtggtctc ggccatgccg
      301 taggcgggca ggatcgcatc gggccgcaga ccgaacggct tgccggcgtc cagcaggtcc
      361 tcgacgtcgg cgggctcgac gggctcggcg cccgacagcg cgaaccgcag cgtcgacagg
      421 tcgaactgac ccggctcagc ttgcttgcgc aaccgcttgg cgaacagcgc gtacgcgaag
      481 ttgggcgccg cggtcatggt gcccttgtac ttgtcgatga gcttggccca cagcagggtg
      541 tcgcgcagga agtccatcgg cgtgaccttc accagctcgg cgccgaagta catcgggatg
      601 gtcaggaagc cgaccatgcc catgtcgtgg aagcagggca gccagctgac catgacgtcc
      661 ttgtcgacgt cgtactcggc gccgatgaac atcgcctcgg cgttggagtg gatgttgcgg
      721 tgcgtgatct ggacggcctt cggcgagccg gtcgagccgg acgtcagctg catcagcgcc
      781 aggtcgtcct cgccgacgtc gacggggtcg atcggatccg acgtcagcag atcggcgatc
      841 tgaaggacct tgatgccctt ctcctcgagc acggggatcg ccgcagtgaa cggctcggag
      901 acgatgacgg ccttggcctc gatcatgccg atgacggtca tggtgtcctc ggcccagatc
      961 accaggtcgg tgcgcggcgt gggctggtgc agcatggtca ggctggcgcc gcgcatccac
     1021 aggccctgcg cggtcggggc gatctcgacc gggaagccgg ccagcacgcc gacggcgtcg
     1081 ccgtggccga tgcccgcggc ggccagcccg cccgcgatgc agcgcgcgcg ctcgtggacc
     1141 tcgccccagg tgtggcggac gggttcgtgg ggttcaccgg tgaccatgcc cgtcgtcacg
     1201 gtgcgggcat tgcggtacat cttctcggtg aatctgctca cgacatcctc ctagacggtc
     1261 ccagagcaaa tgcctgagag taatgctccc gctgatagag ggcgctttcg cggaggcgcg
     1321 cacacaattg gggagcggtt aggtctcgaa tcggggtctc gtctatatat ccgaccaatc
     1381 caccgccggg gttaccggag gctgaaaagg tcgcgcccga ttcctgaccg ctagctttca
     1441 ccgccgatca tcttaagcaa ctcttaagta accgccaaac tgaagcgata attgcgacct
     1501 gggtcacacc cagacgtgat caaaccgacg tcgacggcgc aggcaccact cgggcgccgt
     1561 gaacgggccc gctggcgacc ctcacagtgc ggcacacgcc ggcgcctgcc agctccgcgc
     1621 ccacgtcgac tgcggcggcc gccgacatgc acaggaacgc acacgtcggc ccggaccccg
     1681 acacaatccc ggcgagcgcg cccgcgtcgg cccccgcgcg aagcgtgcgc cgcagccccg
     1741 ggtccaggct cagcgccgcc ggttgcaggt cgttgccgag cagcggggcc aattcggcgg
     1801 gatcacccga ggccaacgcg gccagcaccg gctccggatc gtcgagccgc ggcggctggc
     1861 cccggccttt gtcgcgcaac cggtcgatct cactgaatac cgcgggcgtg gacaggccgc
     1921 cctcggcgaa ggccagcacc cagtggaagg tgctgcgggc gagcaccgtg gcgagctcct
     1981 caccacggcc ggtacccagc gcggtgccgc catgcaatgc gaacggcaca tcgctgccca
     2041 gctgggcggc cagcgcgtgc aggtcgcggc gcggcacgcc gagctcccac agcgtgttca
     2101 tcgccaccag cacggccgcc gcgtcggcgc tgccgccggc catcccgccc gcaacgggga
     2161 tcgacttctc gatggagatc gccacgtccg gcgaccgccc gacgtgctcg gccatcaact
     2221 ccgccgcccg ccaggccagg ttgcgttcgt cggtgggcag cgactccaca ccctcgcccg
     2281 ccatctccaa cgacagcacg tcggcgttgc gcacgatgac ctcgtcgagc agcgacacgg
     2341 catgaaagat ggtcgtcagc tcgtggtagc cgtcgtctcg acgatcaccg acgcccagat
     2401 acagattgac cttgcccggc acccgcacgg tgaccgaccc ggtgggaacc cactcggacg
     2461 cggtacttcc gtcggacgcg gacacggcac gacactatcg ccgggatgaa caggacacgt
     2521 acggaagtgg tcagaagggc ggccgtagcg agccatctcg ccaggtaacc gcccgggcta
     2581 accataggct gtgagggtgc tttcgcccgg cgaccggttc gagggatacg tcgtcgaagc
     2641 ggtgctcggc cacggtgggt ccgcgacggt gtaccaagcc catgactccg ccggtcccga
     2701 tcgtgcggtc gcgttgaaag tgctcgacga gcgccatcgc catcaggcgc aactcgagcg
     2761 tctgcgtcgt gaattcgagt tcgcccacca gctcgaccac gcgcacatcg ttcggatgta
     2821 cgagcgcggg ccgggttggc tttcgatgga gcttgtcccc ggcggtacgg ccgccaagct
     2881 atcgacactg cccgatcggc ttgccgcgct gtcacagatc gccgatgccc tggactacat
     2941 acacaatcgc ggcatcgtgc actgtgacgt caaacccacc aatattcttg tccacccgga
     3001 cttttcccaa aacggagctg tgctcatcga tttcggcgtc gccctttcga tcacctccga
     3061 tatcggatat cacgccaccc acatcgaggc gtccctgccg tattcggcgc cggaactgct
     3121 caccggccac gcaccgagtg aggccaccga cgagtacgcg ctggcatgca ccgccgtcga
     3181 actcatcacc ggttcaccac cgttcacagc aactacccag ctaggcttga tcaaggctca
     3241 tctaagtagc actcctccac gccgatcacg tgagattact tggctgccac gcgttttcga
     3301 ttcgatcctg gccaaggcca tgtcgaaaag cccacagaat cgctatcaat cctgccgcga
     3361 attcgtctcg ttgatcaccc gcgcactgcg ataaacatga gctaaatgcc tacgtaacca
     3421 aacttttacg gtctccgagt ttcgggtccg tgtcgccgca cctaacctct ggccaatggg
     3481 atccgctatc gagtacgaca catctgcgct agctcatgag gacgagggct accacaaagg
     3541 tttgaaaccg agacagctgc agatgatcgc catcggcggc gctatcggca ccggcctgtt
     3601 catgggcgcc ggcggccggc tgcacaccgc ggggcccggc ttgttcatcg tgtacgcggt
     3661 gtgcggtctg ttcgtcttct tcatcctgcg tgccctgggt gagcttgtgc tacaccgtcc
     3721 ttcgtcggga tcgttcgttt cctatgcgcg cgagtttctc ggcgagaagg ccgcgtatgt
     3781 cgcgggctgg atgtacttct tcaactgggc gtgcacgtcg atcgtcgacg tcacaccccc
     3841 gcagggtgct ggagatggcc cgcgagcgag tcggcttcac cggcaattac cccccgttcg
     3901 tcgtcgcgga gacaccgttg gtgacttctc cggcggctga agccgaccgg cttcttctca
     3961 cgcggcttgc gcgcgaaggg ccagtccagc ccgaaggatg ttgcgcgcgg cgtgttcgtc
     4021 agcctgggcg cgatgcgagc atcgctggca gacgaactcc gcttgggtga cgcggttttc
     4081 ggcggctgca tacccgcatt tttcgcagcc gtccgacgtg tgccgagggt cgacctcaat
     4141 ccagatacgc ccagcctctt ccgctttggc gcgcagtatt gagacgaacc ggccccagcc
     4201 ggcgtcactt atgcttcgat tcagcccgga cttcgcccgg gcaccgttct tgaggaactg
     4261 cttcgggttg tccgggtcgg gcaccttggc ccgccgcagc atgttggcga tcttcagatc
     4321 ctccaccacc aacaggtcaa agcgcgctac gagttggcgg gcctgtttgt ggtgaaagtc
     4381 cttgcgctgg ttggcgatct tacgatgccg cgccgcgacc gtttctcgtt tacggtcgcg
     4441 gttcttcgac cgccgttttg cgcactgtag ccgctgctgc gcggcggtca gccggtcgtc
     4501 agcagctcgc ccccatcgcg gattatcgac gcactcacct tcgctggtgg tggcgaaact
     4561 cgcgatcccg acgtcgatac cggcctggcg gccggtggcc ggcagcggat tggtcggcac
     4621 gtcctcacag gacaaaacaa gcagccagtg ccggccgcaa cgcttgacct ggatcgtctt
     4681 gacccgcccc acaacttggc ggtgtaggtg gaccttgacc tggccgatgc cttgcagata
     4741 cacccgcttc tgctcacgca gccagcgggc accgtcgccg tctttgggcc actccacgct
     4801 gtcgaaccga gccttgccct taaaccgcgg aaaccccggt ttgacgccgg gtttggctgt
     4861 cttcactctg cggaagaacc ccgcgaacgc cttgttcaac cggcgcagcg tggcctgctg
     4921 gctggaaaac gaccacacgt ccatatccgg gcgcgctgcc cggatcgcag tcagctgcgc
     4981 cgactgatcc ccgtagtaga tgcgggtcct gctgtgtgac catgcgtcgc gccgttcctg
     5041 cagcgccgcg ttgtagagtt cgcggtgcgc gtccacgcac gccgccaacg cggcgtgctg
     5101 gcgcgcggtc ggccgcagcc tgaaaacgta ggcccgcctc acgatgccac cgcgtcccat
     5161 tggtgctcga tgtagcggcg caccgtcgac tccgagacgt agcccaccga ggcggcgaaa
     5221 caggacggcg accacaacac cttcgccaac cctcgcagac acggaaactc cgaacgcagc
     5281 acccgtgcgg tgcggccctt gaacgcccca accacctgtg ctggggcatc ggttgacccg
     5341 accacacgaa caggtgcacg tgatcgggca tgacttcctt agccacgatc tcccagccgt
     5401 gctcgccggc gatctgttcc agcagctcgc cacaccggac cgcgacgcgg ccacccagaa
     5461 tccgacgccg atacttcggg caccacacca ggtgcaaacc cagcgagcac accccgccag
     5521 gcgtgcgacg aaaccggcgc tcggacatgc cgcaaacgtt acgaaacgac accgacaaac
     5581 tcacgaccac acgctgttca ccaggcccga ttcccccgac gactcaaagc cgccgatccc
     5641 ctcgggccag actagatggc gcggatctgc gactgctgcg ccggctgctc ctggggctgc
     5701 ggttgcggct cctgcggctc cgtgggcttg ggttcccatt ctccggagcg ctgcagcagc
     5761 cggacgaagt ccgcgaccga cagcgtctcg ccgcggcggg ccgggtcgat gctggccgcc
     5821 agcagccggt tggcggactc gttgcccgaa cccgcccact cggcgaacgc gttgcgcgat
     5881 gtcttgcggc gctgcgaaaa cgccacatcg atcagctcga acaccttggt gcggaaatca
     5941 gcgtcggtcg gccacggcga ggcctcgtgg cggtcgatac gcaccagtcc cgaatacacg
     6001 cgtggaatcg gccagaacac tgtcggagac accatgccgt agcgccggac gttgccgaag
     6061 aaccgcacct tgacgctggg cacgccgtac tccttgccac cgggttcggc ggcgagccgc
     6121 tcggcgacct cggcctgcac catcaccatg acggtgcgga tcgtcggaaa ctcggcgagt
     6181 agatgcaata gggcgggaac ggcgacgttg tacggcaggt tggccaccag ggccgtcggc
     6241 tgctcctcca cgtctggcgg gtgcagcgtc aggatgtcgc ggttgagcac ggccagccgg
     6301 ttgatctcgc tgtgcgaatg ctcggcgatg gtcttcggaa gctgccgggc cagaatcgga
     6361 tcgatctcga cggcggtcac tctcgcgccg cggtcgagca gcgccagggt caacgaccca
     6421 agccctggac ccacctcgag gacgtggtca tgacgattga cgccggaggc cgaaacgata
     6481 cggcgaacgg tgttggcgtc gtggacgaag ttctggccga aagatttgcg cggcctgaag
     6541 tcaatctctt tcgccaggtg tcgtatctcg gtccgcccga gcagtcgaat agtcagcgcg
     6601 ctccagtcct cccgctacac acgggccacg cgccccaacc ttgtcgcgcc cgagtgacct
     6661 cagcaatcgc gatctgttct tctcttgtcg ccagatccgc cctcggagca tacctcagac
     6721 cgccttgtcg ttcccaggtg ttttgatcaa attggacgcc accgtagaac ccgttgccgg
     6781 tgttgatggc ccaattacct ccggcttcgc acttcgacag cgagtcccag acagctccat
     6841 ttgttaccgg aggaacttcc gtccccggtt tcgcgccgac gcggagcacc ccgtcgcggg
     6901 ccggcgcgat aacgacattg gctactggca accttccggt ctcgactcca ttgaccgtcg
     6961 agacggcgaa tgtgacgtcc tgtgtgccgg gcatcccggg gtcgtcgacc acctgacggc
     7021 tcatgttcat ggtggcgtcg gggatttgct ggttgttcgg tggcagcggg aggcgctggg
     7081 tcaccttgtc gatgcggatc cgggtcacct ggacctgcat gttgtcgacc accggcgacc
     7141 atgcgggggg gaccacggtg tcgttctgct taaggggcac acccgcagcc tcgagcagtc
     7201 cggcgacgtt cggcgccgca agatggattg tccgggtcgc gccagcgtcg ttgagctgca
     7261 ccgtctttgc gctgacaacg ggaagggaca tacccgccag tgggacgcga ctgccgcgcg
     7321 atgcggctgc cggtgccttg tcggtcatct tcagctgggc cagcgcctcg tcgactgtcg
     7381 acgccgtcgt ccacacctgc ctgctgttct cgccgtcgag cgagatctgc aggggccggc
     7441 tgcggcgcag cacgatcgag tcggactgac gcacttgctc gtttgcggct gggtagaggt
     7501 cgtcgcggtc gccgacgtcg aagccgttct ccttgaccac atcgatcaca cgcgacttca
     7561 tcgtggcaac tgtcatctcg gagccgtcga cggtcagttt gacggtcttg tgcgcggcaa
     7621 cggcgtatcc gcctgcgaag gtcagcgcga gcaacgtcgc accgaccagc agccgaagca
     7681 acggcgagcg cgcctcgtgg agcttattca aagtattcaa aatgtggtct atccccgact
     7741 atcggacggt tgacggctag gccggttccc cccgttcgat caccgaacgg taacggttag
     7801 gtcaaggggc ggcaactcgc cagcccataa gcccggatgg ccgccgtagt acttgcctgg
     7861 gcaatgtctt ctgccgggcg gtcgacgact tcggcgagcg ccctcaccgt gtagggcagg
     7921 cagtacggct cgttcggggc accacgatac gggtgcgggg tcaaaaacgg cgcatcggtc
     7981 tccacgagca gctggtcagg cgggatcagg gcagccgcct cccgcagcgc cttagcgttc
     8041 ttgaagctga cggttccaga caggctgagc agccagccgc tggccacaca ggtgcgggcc
     8101 atggccgcgt ccgacgagaa gcagtgaaag atcaccgtgt cgggtgcgcc ctcggcttgg
     8161 aggacgtcga gcacttgggc gtcggcgtcg cgattgtgga tcatcagcgg cttgccgatg
     8221 cgcttggcga ggtcgatgtg ccaggcgaag gcgtccttct gggcggcggg ctcggcgcag
     8281 ccgtccagct tgccgggcca gtacatgtcc atgccggttt cgccgaccgc gacgacgcgc
     8341 gggtgttcgg ccagccgctc gatgacggtc ttggcttcgt cggtgagcgc attggcgcgc
     8401 gtcgggtgca gcgcgacggc ggcgtagacg cgatcgtccc aatcggccgc ctcggtcacc
     8461 cagcgggcgg aatccaggtc gtcggcgatc gtgacgacgg cttgcacgcc gacggccgcg
     8521 gcgcggtcga cgatcgccgt cacgctcgcg gcgtcggtgg ccccgcatgc gtcgaggtgg
     8581 gtgtgggcgt cgatcagcgg gctcagtggc tccggtgcgg gcggcgccgg gcgcttctga
     8641 tccacgccaa cacaataagg tgaggacaaa tgagcgagcc gtactacatc acgacagcca
     8701 tcgcctatcc caacggcgat ccgcacgtcg ggcatgccta cgagtacatc gcgaccgacg
     8761 cgatcgcgcg gttcaagcga ctcgacgggt tcgacgtgcg cttcctgacc ggcaccgacg
     8821 tgcacggaca gaagatggcc gagacggcgg cggccgaggg catcccggcg gccgagctgg
     8881 cgcggcgcaa ttccgacgtg ttccagcgga tgcaagagcg gctcaacatc tccttcgatc
     8941 ggttcatccg cacctcggat gccgaccact acgaggcgtc caaggagatc tggcgccgga
     9001 tgaacgaggc cggcgacatc tacctggacg cgtacaaggg ctggtactcg atacgcgacg
     9061 agcggttttt caccgaggcc gaaacgaccg ttggcgccga cggcgtgcgg atcgccaccg
     9121 agaccggcgc cccggtgacg tggaccgagg agcagacgta cttcttcaag ctgtcggcct
     9181 atgcggacaa gctgctggcg ctctacaagg cgcacccgga gttcatcgag cccgatgtgc
     9241 gccgcaacga ggtggtcagc ttcgtctccg gcgggctgcg cgacttgtcg atctcgcgga
     9301 ctacgttcga ctggggcgtg ccggtgcccg accaccccga ccacgtgatg tacgtgtggg
     9361 tggacgcgct gacgaattat ctgacgggcg tgggttttcc ggacacgtcg tcggacttgt
     9421 tcaagcggtt ctggccagcc gatctgcaca tgatcggcaa ggacatcatc cggtttcaca
     9481 cggtctactg gccggcattt ctgatgtcgg cgggaatcga gttgccacgc aaggtctttg
     9541 tgcacggctt cctgctcaac aagggtgaga agatgagcaa gtcggtgggc aacgttgtcg
     9601 acccgctgaa cctgatcgac acgttcgggg tggaccaggt gcgctacttc ttcttgcgcg
     9661 aggtgccgtt cgggcaggac ggcagctaca gcgaagaggc gatcatcggc cgcatcaacg
     9721 cagacctggc caacgagttg ggcaatctcg cgcaacgctc gctgtccatg gtggcgaaga
     9781 atctggacgg aatcgtgccg gagccaagcg aattcaccgc cgacgacaag gcactgctgg
     9841 aggcggccga tgcgttgatg gaccgtgtgc ggagccatta cgacgccacc gcaatgcatc
     9901 tcgccctgga atcgatctgg tcggtactgg gcgcggccaa caagtacttc tccgcacagg
     9961 agccgtgggt gctgcgcaag accgatgagg agcggttccg cacggtgctg tacacgaccc
    10021 tggagacggt ccggatcgcc acattgcttt cgcagccggt gatgccggag tcgacgggca
    10081 agctgctcga cctgctcggg cagaccgacg atcagcggac gttcgctgcg atcggaagcc
    10141 ggttggcgcc gggaaccgcg cttcccgccc cggttggggt gttcccgcgc tatcaagcgg
    10201 agtgacatag ccgctcgcgg cttaatgctg gcggtaccgc caggacacgg cggggtaacg
    10261 gctgcctact tgcattgagg gcatgaccga catgttccat ttgggctggt ttctgaactt
    10321 cgtcgccgac gaatggaacg gcaactgggg tgacggcggc cgcgatttca ccggggactt
    10381 ctacgtggaa atcgctcgcg atctcgagcg tggcaggttc gactacgtcc tgcttgagga
    10441 caagctcatg gtgtcgaccg cctacggcgg cacgatggag cacgacctca agcacggggt
    10501 taaccccaaa cacgaccctg ttccgctggc cgtgctgatg gccaatgcca ccagccgcct
    10561 aggcgtgatc ccgacgatgt cgaccacctt ttatccgccg ttcctattgg cgcggctgtg
    10621 cagcaccatc gaccacatcg cccgcggacg gttcggctgg aacgtcgtca cctccggcga
    10681 ggaccgctcg gcacagaact tcggtatgga caagttgtac gaacacgacg atcggtacgc
    10741 gagggccagc gagtacatgg aagtggtcac caagctgtgg gagtcgtggg agcccgacgc
    10801 gatcgaacgc aacaccacca caggcacatt cgcgaatttc aagaaggtcc acacgatcga
    10861 cttcgatggc a
//
LOCUS       DASRJA010000632         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_641467,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000632 DASRJA010000000
VERSION     DASRJA010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641467"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VFC01_28335"
     CDS             <1..709
                     /locus_tag="VFC01_28335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF-domain containing protein"
                     /protein_id="HZN83560.1"
                     /translation="FPTGYSTESRTRLLLDSLIITAALFEISWVLVLEDVWNAGAGSR
                     FALGLSLAYPVSDIVVVSVALLVLAKARTGQRLTLMLLTAGSLLNALSDSAFVYLTMH
                     GAYSGGGLTDVGCAAALALLAMAALVGRRTPQAEEAETWVPPRPAQWLPYAPLAVAAA
                     MCIPRYLFEPGLTAIFASSAFLMTAVLARQFVVVRHNRRLLEVVADQALRDPLGLSEP
                     SPVSRSADARVATSPAG"
     gene            687..1763
                     /locus_tag="VFC01_28340"
     CDS             687..1763
                     /locus_tag="VFC01_28340"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HZN83561.1"
                     /translation="MQLHQRDDQSVAVVSLDLNDFKSVNDSLGHPAGDALLVQVAQRL
                     VDCVRTGDTVARLGGDEFAVLLEGRAHQSRLIAHRVVQAFDERFVVDGHDLLMRPSVG
                     LAMVTADAKISTDTLLKRADTAMYAAKRSRSGDVHTFTPEMHRPDGNDCGGDGARLLD
                     QLRSAIDNVDLTLVYQPKFDLRDGAIVGVEALVRWPHPELGMLGPDHFLPLVRRHGLI
                     RSVTELVLAGALDDAADWRARGVEVPIAVNISAPSLGDLDLPGRIERALVDRDMSARS
                     LTVEITEDLLVDNVDRTRTVLDDMRARGIRVAIDDFGSGYSALSYLRDLPIDEVKLDR
                     QFITPNTCRPAIRGDRPRRDRPFA"
     gene            1744..2010
                     /locus_tag="VFC01_28345"
     CDS             1744..2010
                     /locus_tag="VFC01_28345"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN83562.1"
                     /translation="MIDLSHELGVTTVAEGVENEETADRLRDYGCEFVQGYYCSPPVG
                     AADIVDLIHGNKPCCPEDTAALGLNAGDFRAHRRIKLAGLSSRL"
     gene            2007..2750
                     /locus_tag="VFC01_28350"
     CDS             2007..2750
                     /locus_tag="VFC01_28350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN83563.1"
                     /translation="MTAPVDPNRFEEMYRDERTAHGLPAATPWDIGGPQPVVQQLVAL
                     GAVKGEVLDPGTGPGHHAIYYASKGLSATGIDASPAAIDRAEQNAQKAGVKVDFRVAD
                     ATKLEGLDDRFDTVVDCAFYHVFSTEPELQKSYARALHRATKPGARLYMFEFGAHNVN
                     GFKMPRSLSEDDFRQVFPDAGWDITYLGPTTYQGNVSVETFEMMAARNPDMAEEMNPL
                     LERLRVIEPWLVDGRVHMPFWEVHATRVD"
BASE COUNT          521 a          974 c         1038 g          599 t
ORIGIN      
        1 gtttcccacc gggtactcga ctgagtctcg aacccgcctc ttgctcgaca gcctcatcat
       61 caccgccgcg ctcttcgaga tctcgtgggt cctcgttctc gaagatgtct ggaatgcggg
      121 tgccgggagc cgtttcgcac tcggcctttc gttggcctat cccgtcagcg acatcgtggt
      181 ggtgtcggtg gctttgttgg tgcttgccaa agcacgtacc ggccagcgcc tcacgctgat
      241 gctgctgacc gcaggcagtc tgctgaatgc attgtccgac agcgcgtttg tctacctcac
      301 tatgcatggc gcctattccg gcggtggcct aacggatgtg ggatgcgcag cggcgctggc
      361 gttactcgcc atggccgcac tggtcggccg tcggacgcca caagctgaag aagccgagac
      421 gtgggtgccg ccgcgccccg cacagtggct gccatatgcg ccgctcgcgg tggccgccgc
      481 aatgtgcatt ccgcggtacc ttttcgaacc gggattgacc gcgatcttcg cgtcgtcggc
      541 gttcttgatg acggccgtgc tggcgcggca gttcgtcgtg gtacgccaca atcggcgtct
      601 gcttgaggtg gttgccgatc aggctttgcg cgatcctctc gggcttagcg aaccgagtcc
      661 tgtttcacga tcggctgacg cacgcgttgc aacttcacca gcgggatgac cagtcggtgg
      721 cggtggtatc actggatctc aacgatttca agagcgtcaa cgacagcctc gggcatccag
      781 ccggtgacgc gctgctcgta caggtggcgc aacggttggt cgactgcgtg cggacaggcg
      841 acacggtggc ccggctgggc ggcgacgagt tcgcggtcct gttggaagga agggcgcacc
      901 agtctcggct cattgcgcac cgggttgttc aggctttcga cgaacggttc gtggtcgacg
      961 ggcacgacct gttgatgcgg cccagcgtcg gcctggccat ggtgacggcc gacgcgaaga
     1021 tttcgactga caccttgctg aaacgcgccg acaccgcgat gtatgcggca aagcgctcgc
     1081 gctcgggtga cgtgcacacg ttcactcccg aaatgcaccg acccgatgga aacgactgcg
     1141 gcggcgacgg tgcccgactt ctagaccaat tgcgcagcgc gatcgacaac gtggacctga
     1201 cgctggtcta tcaacccaag ttcgacctgc gcgacggcgc gatcgtcggg gtggaggcac
     1261 tggtgcggtg gccgcatccc gagcttggca tgctcggccc tgaccacttt ctgccgttgg
     1321 tccgccgcca tggccttatc cggtcggtga ccgaactcgt gttggccggt gcgctggacg
     1381 atgccgcgga ttggcgtgca cgcggcgtcg aggtgccgat tgcggtaaat atctccgccc
     1441 cgtcgctcgg agacctcgac ctccctggcc ggatcgagcg cgcgctcgtc gaccgcgata
     1501 tgagcgcgag atcgctgaca gttgagatca ccgaagacct cctggtcgac aatgtagacc
     1561 gaacacgtac ggtgctcgac gacatgcgag cgcgcggaat ccgggtggct atcgatgatt
     1621 tcggcagtgg ctactcggcg ttgtcgtacc tgcgcgacct cccgattgat gaagtgaagc
     1681 tcgataggca gttcatcaca ccgaatactt gtcgacccgc gatccgcggc gatcgtccgc
     1741 gccgtgatcg acctttcgca tgaactcggc gtgacgacgg tcgcagaagg agtcgaaaac
     1801 gaagaaacgg ccgaccggct ccgcgactat ggctgcgaat tcgtccaggg ctattactgc
     1861 agcccaccgg tcggggcagc ggacatcgtc gacctgattc acggtaataa gccgtgttgc
     1921 cccgaggaca cggccgcgtt gggcttgaat gcaggcgatt tccgcgctca ccggcgcatt
     1981 aagttagcgg gtctatcctc gcggctatga ctgctcctgt agatcccaat cgcttcgaag
     2041 agatgtaccg cgacgagcgg acggcgcacg gattgcccgc cgccacaccg tgggacatcg
     2101 gcggtccaca gccagtggtt cagcaactcg tcgcgctggg cgccgtcaag ggcgaggtgc
     2161 tcgaccccgg caccggcccc ggacaccacg cgatttatta cgcgtcaaag gggttgtcgg
     2221 ccaccggcat cgacgcgtcg cccgccgcca tcgaccgcgc cgagcaaaac gcgcaaaagg
     2281 ccggggtaaa ggtggacttc cgggtcgccg acgcgaccaa gctcgaggga ctcgacgacc
     2341 ggttcgacac cgtcgtcgac tgcgcgttct atcacgtatt cagcaccgag cccgagttgc
     2401 agaagtccta tgcgcgggcg ctgcatcgcg ccaccaagcc cggcgcgcgg ctgtacatgt
     2461 tcgagttcgg cgcgcacaac gtgaacggct tcaagatgcc gcgatcgctc tcagaagatg
     2521 atttccgcca ggtgtttccg gacgccggct gggacatcac ctatctcggg ccgacgacgt
     2581 accagggaaa cgtcagcgtc gagaccttcg agatgatggc cgcgcgcaac ccggatatgg
     2641 ccgaagagat gaatccgttg ctggagcggc tgcgggtcat cgaaccgtgg ctggtcgacg
     2701 gtcgagtgca catgccgttc tgggaggtgc acgccacccg cgtcgactag ctggctgtag
     2761 gtcgtgtcaa gtccgggtaa cgggtgtgcg gcgttagcaa cccgggcggc ccccggtttg
     2821 ttggggtgtg tcggtgttgg ggcggtttgt gtgggtgagg agttgtttcc tcggcggtca
     2881 gcatgcgccg gtgctcgtcg ctgtccacgt cgttcgtcct cgctgtcgct gcgggcggtg
     2941 cacgctggac agctcctgcg ccgccggcgc cgatggccca ggaccgagga cagccaacac
     3001 gcgatgcttt aggtccagcg ggcggactcg taacggctgc ggtcccggac cagggcatag
     3061 gcgacgcggg tggcgcgatg cgccagggca caggcgatga tgccgccgtg tttaccgcgg
     3121 gctttcagtc cg
//
LOCUS       DASRJA010000633        10011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1057046,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000633 DASRJA010000000
VERSION     DASRJA010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10011
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057046"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2639
                     /locus_tag="VFC01_28355"
     CDS             <1..2639
                     /locus_tag="VFC01_28355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009473015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZN83564.1"
                     /translation="KTALLQYLVGRATDCRVLQTAGVESEMELAFAGLHQLCMPLVAG
                     LNDLPQPQGDALRVVFGTGSGPAPDRFLVGLAALGLLAHAAEQKPLVCIIDDEQWLDR
                     ASAQALGFVARRLEAESVGLVFAARVPTPDVAGLSELSVAGLDAADAHALLDAEVTAP
                     LDRWARDQLVIETRGNPLALLEWPRALTTQQWAGGFGLPAAVRLPGGVAESFRTRLQM
                     LPEPTRRLLLIAAADPLGDAGLVWRAAAQLGLDAEAAEPAIEVGLVEFGTRVLFRHPL
                     VRSVVYTTASLHDRRLVHDALAQVTDAQHDPDRHAWHRAHAAAGPDEKVAAELERSAA
                     RAQARGGLAAAAAFLHRATELTLDREQRAERALSAASAQAQAGGLDAARDLVSLAKAQ
                     APSGFQQARIELIEAELAYTTTRGGDAALLLLKAAQRLESIDAGLARSTYLRAMSAVF
                     FSDDVSRGVVGLQVAQAAAAAPRPTRTPNAADLLLDGFAAHYTQGYQAGAPILRRAFD
                     EFSTGMSPEMELHEQLLANFLAAQHLWDHHRWQVLSDRYLELARSLGALSELPMALTS
                     KVFWLAFAGELSAAAALSQEVQAAVEATGSSIGPYAALLVAAMRGRPGEVTDLIDAAR
                     SDLNRRRDWLGLTMADYTSAMANNAVGNYQEVIAASVRSASHRLSSSSRSRLSVRGAW
                     QALEVAEAAVRCGEADLAADAVGKLARVTSASGTDWACGVEARCRALVTDGPAAEDLF
                     RESIKRLGRTQLRPDLARAHLLYGEWLRRERRPSDARAELRVALDMFEAMDMEGFGGR
                     ARRELEATGETRRKRTVATDPQMLTAQEANIARLARDGHSNPEIGARLFISTRTVEYH
                     LQKVFTKLDIQSRGQLHRVLAD"
     gene            2903..4165
                     /locus_tag="VFC01_28360"
     CDS             2903..4165
                     /locus_tag="VFC01_28360"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN83565.1"
                     /translation="MTAHSSTSVIESASGTDDPDACASPANVGELSDLTIDEGSLDGL
                     LAAGEFAAEFDENLDEGRSHADGRRLVAEQAVLRRLAMMVARGVGPSAVFGAVAGEMR
                     RCVPAITAGLWRFEADGEITIVAAAADPATYEKWPVDTRIPDEGNNVATSVQRNGQRE
                     RTASADVADHCGAQKRQFLAEASERSNLIDALIEGRALDEWSLREVAGRLRLPMNGPF
                     VVVAADVPTVGDEPLAEIDSKLRSLDIFSAWRLLPDVQVGIVHIASDRKLGMVVSLLS
                     RLTTARVGVSATFADLRDTPRALHVARVMLRSPTDSTSSIAVFDGSILATAAVSAPAM
                     MIKSAGTVLERFSDLPEAEREALFETFRVWQDNDASINVTADRLFCHPNTVRQRLRRI
                     EKHTGRSLSRPRDVAELCLTFEVRRHLM"
     gene            4206..5018
                     /locus_tag="VFC01_28365"
     CDS             4206..5018
                     /locus_tag="VFC01_28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007538401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN83566.1"
                     /translation="MPKQIEYLQGTPNWVELQTSDQSAAKEFYGSLLGWSFDDQLLPQ
                     DGIYSMATLRGEAVAAIAPIPPTASDAMSPRWNTYIAVDDVNATSETIAPAGGQVLTE
                     PCDVGDAGRMTWVTDPTGALVGLWQASRHIGATLVNEPGALFWNELLTDKPDTAIAFY
                     EAVLGVTHVSVDTPSGTKYHSLKVAGHDVAGCVQPPMHGIPNHWHNYFAVDDADATAH
                     KAVAHGGRIAVDPFDIPWTQLLPRDVGAAVGRAAVVADPQGGLFGVMTMPPP"
     gene            complement(5059..5712)
                     /locus_tag="VFC01_28370"
     CDS             complement(5059..5712)
                     /locus_tag="VFC01_28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN83567.1"
                     /translation="MTAQLHSPVAESARSTVDPDTHACGADGGELIARYQSDVIPLRE
                     PLYRQAMRMTRNHADAEDLVQDTLMKAYAGLHTFQQDNNLRGWLFRIMRNAYITTYRK
                     ERRRPVHYMPGHFTEELQVTAIHESTTGTRSAEQHALERLGDNKIREAMCALPDQFRM
                     AVYYADVEGFSTKEIADHMQTPVGTVTSRLHRGRRLLRDLLADVAEQRGDTTVDDAA"
     gene            complement(5988..7598)
                     /locus_tag="VFC01_28375"
     CDS             complement(5988..7598)
                     /locus_tag="VFC01_28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005111312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN83568.1"
                     /translation="MFSHPIQEKAYGEVAAALPFAIDPSKITVEPGVSYLPSPIKLHD
                     YAVGVMAAFGSVVEHLGRIRGLPAQTMTLNRRLCGFHLNELQTQFLNGYALTLDTWPM
                     AADNGTYRTKDGRYVQMIGLFPHLAKGLTARLQCADTPEAIQAAVEKYTAQELEDEFA
                     GLNLALGMVRTPQEWLAHPQGQATAKLPVFGIHQQGTTKNRALGTAKFRPLEGVRVVD
                     LTNVVAGPTAGFALAEQGADVILVHSPHGDWVTPIWMSVNWGKKSILTDIKSPDGKKR
                     FIDLLASADVLISSQRPGALDRLGLSEAELREINPNLVYGSESFAPPGTPWAGRRGFE
                     QIAQAVTGTMHVHSEGLGLDAPTVTGGLMNDHITGYLLAIAVVAALAEREEKGGFWHV
                     DASLMRTSMLGNQLVGPVDDEPYAPVTVQDLIEYGVDQDSPWGTFTRFTPPVGFSHTP
                     SMALRTPPWPGTHPDTIGWDADPVGDEAPEVPHYPSKLAREGGIRNLVPNFGIEDRGD
                     GGGVVGLVSKPEALEAQLKKWAAADVSA"
     gene            complement(7784..>10011)
                     /locus_tag="VFC01_28380"
     CDS             complement(7784..>10011)
                     /locus_tag="VFC01_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZN83569.1"
                     /translation="TFVRVGAMAPDAVADMVCDTVRANADASLLTLAAKAHGNPFLVS
                     ELVGGLGEEGRLTVSGGRAVAAGDGLPRRLSATMQQRLDLLSNDAGEMVRVAALLPDR
                     FSAGLLAAMLDRPPVSLLSGLEEAVRADLLVEDGERLRFRHDLLREATRQSIPQSLRR
                     AMERQCASIMLGMGAAPAEVATQLARSAEPGDREAVSALRQAARSVSRSDAGTAADLS
                     KRALELLPGQDPQHGRLVAETVELLNRARRYEEAEELAVAALSEAASPEAEAEIRLRV
                     MTLTKHSTQRRVEENRRALQLGEINEVTRARHLAWLAYNLALHDQTGERRPAADEAAE
                     AAEGTGDLEATILADVTLALLDAADGYPGRALHPLEKACALARTSYATAARDLAAQHY
                     ALLLAAVGRVDEATAQIASGTAQVRQEGNAMAVNIWAVDAGLVHLAAGRLSAARTAVE
                     FLPPAEPAPTDTDMLRVMILAEVAARTDDRTLLQQMVNSAREAYSARSSLLGRTAAHV
                     LALAAWQRGDVHDAMRWFGDVSLVGPPVGPATLDRAVFCARIASAGGDAGLRARVLQV
                     TDLLERERPANPLFSGIAAYVRGILERDAAVLVAAAESLQSSSRPLLYAAAAEDAGDE
                     LARIGRGDEALSQLNAAFDTYTRHEALADARRVGRQLRTLGVQRRIVGQQRARTGWDS
                     LTDSELRVVNLIAGGATNRDVATQLHLSVHTVKTHVHNAFAKLGITSREQLAPLMRGA
                     D"
BASE COUNT         1670 a         3212 c         3350 g         1779 t
ORIGIN      
        1 gcaagacggc gctactccag tacctggtag ggcgggcaac ggactgccgg gtactacaga
       61 ccgccggcgt ggagtcggag atggagctgg cgtttgccgg attacatcaa ttgtgcatgc
      121 cgctggtggc cggcctaaac gacttaccgc agccccaggg tgatgcgttg cgggtcgtgt
      181 tcgggacagg ttcgggaccg gcgccggatc ggtttctggt tggcttggcc gcgctgggcc
      241 tgctggccca cgccgccgag cagaagccgc tcgtgtgcat catcgacgat gagcagtggc
      301 tggatcgcgc ttcagcacag gccctcgggt ttgtggcgcg ccggttggag gccgaatcgg
      361 tggggttggt gttcgccgcg cgcgtcccca cgccggatgt ggctgggctg tcggagcttt
      421 cagtggcagg attggacgcc gccgatgcgc acgcgctgct agatgccgag gtgaccgctc
      481 cgctggatcg ctgggcgcgt gatcagctgg tgatcgaaac ccgagggaat ccgctggcgt
      541 tgttggagtg gccgcgggcg ctgacgacgc agcaatgggc gggcggtttc gggttgcctg
      601 ccgcggtgcg gctgcccggt ggggtggccg agagctttcg gacgcggctg cagatgctgc
      661 ccgagccgac ccgcaggctg ttattgatcg cggcggccga tccgctcggc gatgcgggtt
      721 tggtctggcg ggctgccgcg cagttgggcc tcgacgccga ggccgctgag cctgccatcg
      781 aggtcggtct tgtggagttc ggcacccgag tgttgtttcg gcatccgttg gtgcgttcgg
      841 tggtctacac cacggcctcg ctgcatgacc ggcggctggt ccacgatgcc ttggcgcagg
      901 tcaccgacgc ccaacacgac cccgatcgtc atgcgtggca ccgcgcgcat gccgcggccg
      961 gacccgacga aaaggtggcc gccgaattgg agcgttcggc ggcgcgggcc caagcgcggg
     1021 gcggattggc cgcggcggca gcgtttttgc atcgggcgac cgagttgacg ctggaccgcg
     1081 aacagcgcgc cgaacgggcc ctcagcgctg catcggcgca ggctcaagct ggcgggctcg
     1141 atgcggcgcg tgacttggtg tcgctcgcaa aagctcaggc gcccagcggt tttcagcagg
     1201 cacggattga gctgatcgaa gccgagctgg cgtacaccac cactcggggc ggtgacgcgg
     1261 cactgctgct actgaaagcc gcccagcggc tggagtccat cgacgcgggg ctggccaggt
     1321 cgacctacct gcgcgctatg agtgccgtgt ttttcagcga cgacgtgtca cgcggtgtcg
     1381 tgggactgca ggttgcgcag gccgccgcgg ccgctccgcg gccaacgcgc acacctaatg
     1441 ccgctgacct gctgctggat ggcttcgccg cccactacac ccagggatat caagcgggag
     1501 cgccaatcct acgcagggcg ttcgatgagt tcagcaccgg catgtcgccc gagatggagc
     1561 tgcacgagca gttactcgcc aacttcctag ccgcccagca cttgtgggac caccaccgct
     1621 ggcaggtgct gtcggaccgc tatctcgagc ttgcccgaag ccttggtgcg ctcagcgagc
     1681 ttccgatggc cctgacatcg aaggtgttct ggctcgcgtt cgcaggcgag ctcagcgccg
     1741 ccgctgctct gagccaggag gtacaggccg ccgtagaggc gaccggcagc agtatcgggc
     1801 cgtacgcggc tctactggtg gccgcgatgc gcggccgacc cggcgaagtg accgatctga
     1861 tcgacgccgc gcggagtgac cttaaccgtc gaagagactg gttggggtta acgatggcgg
     1921 actatacgag cgcaatggca aacaacgctg tcggcaacta ccaagaagta atcgcggcat
     1981 ccgtgcggtc cgcgagtcat cgtctgtcat cgtcgtcacg cagccgactg tctgttcgcg
     2041 gtgcctggca ggctcttgaa gtcgccgagg cggccgtgcg ctgtggagag gcggaccttg
     2101 ccgccgatgc cgttggcaag cttgcccggg taaccagtgc cagcggcact gattgggcct
     2161 gcggcgtgga ggcccggtgc cgggcactcg ttaccgacgg accagccgcc gaggatctgt
     2221 tcagggaatc catcaagcgg cttggccgta cccagctgcg cccggatctg gctcgcgcac
     2281 atctgcttta cggtgaatgg ctgcgccgcg agcgccggcc cagcgacgcg cgcgccgagc
     2341 ttcgcgtcgc cctcgacatg tttgaggcga tggacatgga gggattcggc gggcgggcgc
     2401 gtcgcgaact cgaggccacc ggcgagaccc ggcgcaagcg gaccgttgct accgacccgc
     2461 agatgttgac cgcgcaggaa gccaacatcg cgcggctggc ccgcgatgga cattccaacc
     2521 ccgagatcgg cgcgcggctg ttcatcagca cccgcactgt ggagtatcac ctgcagaaag
     2581 tcttcaccaa gctcgacatc cagtcgcgcg ggcaactgca ccgcgtcctt gctgactgac
     2641 ccagcacccg tgccctggcg tagccgcaac cactcaacca gggcacgact gtggattagc
     2701 actgcggggc ttacggatgt taacggcctt acgggaccgt gatgatcacc cttacaacaa
     2761 accgttgtcg atcgccctca ctgggcgtca ccgcaccagt gagtagacga gggaaagggt
     2821 gaacaatgat gagtacagcg tttggaatgc tggcgatgct gccggtggtg ttcgtcgccg
     2881 ctgggtatgt gttggtgtcg cgatgaccgc ccactcgtct acctcggtca tcgagtctgc
     2941 cagtggcact gacgatccag acgcatgcgc cagcccggcg aacgtcggtg aactgagtga
     3001 cctcacaatt gatgagggtt cgctggacgg actcttggcc gctggggaat tcgccgccga
     3061 attcgacgag aacctggatg agggtaggtc gcacgccgat ggtcggcggc tcgtcgccga
     3121 gcaggccgtg ttgcgcagat tggccatgat ggtggcgcgg ggagtcggac cttcagcagt
     3181 attcggcgct gtggccgggg agatgcgtcg atgcgtgcct gcgatcaccg ccggattgtg
     3241 gcgcttcgaa gccgacggcg agatcacgat cgtcgccgcc gctgccgatc ctgcgacata
     3301 cgaaaaatgg ccggtggaca ctcgaattcc ggacgagggc aacaacgtcg cgacgtcggt
     3361 gcaacgcaac ggccagcgcg aacggacagc cagtgcggac gtcgcggatc attgcggggc
     3421 gcagaagcgg cagtttctgg ctgaagcgtc cgaacgttcg aatttgattg acgcgctgat
     3481 tgaggggcgt gcgctcgacg agtggagtct gcgggaggtc gccggccgtc tccggctgcc
     3541 gatgaatggt ccgtttgtgg tcgtcgccgc agacgttccc actgtgggcg atgagccgct
     3601 cgccgagatc gactcgaagt tacgtagcct tgacatcttt tcggcgtggc ggctgctgcc
     3661 ggatgtgcag gtcgggatcg tgcatatagc gtcggaccga aagctcggca tggttgtttc
     3721 cctgctatcc cggctgacaa cggcgcgggt gggggtgagt gcgacgttcg cggacctgcg
     3781 cgatacgcca cgagccctgc atgtggcaag ggtgatgttg cgcagcccga ccgattccac
     3841 atcgtcgatc gccgtgttcg acggctccat cctggccacc gcagcggtga gcgcgccagc
     3901 gatgatgatc aaatcggctg gcaccgtatt ggagcgattc agcgaccttc ccgaggcgga
     3961 gcgagaggca ttgttcgaaa cgtttcgggt gtggcaggac aatgacgcgt cgataaacgt
     4021 cacggccgac cgactgttct gccatccgaa taccgtgcgc cagcgtttgc gtcgcatcga
     4081 gaagcacacc ggccgatccc tttcgcggcc aagggatgtc gccgaacttt gtctcacctt
     4141 cgaggtgcgg cgccacctga tgtagccgcc tgcccgtgca tcagtcatta acgagaggtt
     4201 ggatcatgcc gaagcaaatc gaatacctgc aaggcacccc gaattgggtc gagctgcaga
     4261 ccagcgacca gtccgccgcc aaggagttct acggatcgct gctcggctgg agcttcgacg
     4321 accagctgct tccgcaggac ggcatctact caatggcgac gttgagaggg gaagcggttg
     4381 cggcgatcgc gccgatcccg ccgacagcat ccgatgcgat gtcaccgaga tggaacacct
     4441 acatcgccgt cgacgatgtc aacgcgacgt cagaaacaat cgcaccggca ggcgggcagg
     4501 tcctcacgga gccctgcgac gtcggcgacg ccggccggat gacctgggtc accgacccga
     4561 ccggcgcgct cgtggggctg tggcaggcca gtcgccacat cggcgcgaca ctggtcaacg
     4621 aacccggcgc actcttttgg aacgagctgc tcaccgacaa acccgatacg gcgatcgcgt
     4681 tctatgaagc agtgctcggc gtgacccacg taagcgtgga caccccttcg ggtacgaaat
     4741 accactcgct caaggtcgcg ggccacgacg tcgccggctg cgtgcaaccg cccatgcacg
     4801 gaatacccaa ccattggcac aactacttcg cggtcgacga cgccgacgcc acagcacaca
     4861 aggcggtcgc gcacggcggc cggatcgccg tggacccgtt cgacattccc tggacgcagc
     4921 tgctgccgcg agacgtgggc gcagcagttg gccgagccgc cgtcgtggcc gacccgcaag
     4981 gtggcctctt cggcgtaatg acaatgcccc caccgtagga gggctcatct gtgcggtttg
     5041 gcgccgagct cagtggcgct acgccgcatc gtcaaccgtg gtgtccccgc gttgttcggc
     5101 gacgtcggcc aggaggtcac gcaggagacg ccgtccgcgg tgcagccggg acgtcaccgt
     5161 gcccaccggg gtttgcatgt gatcggctat ctctttggta ctgaagcctt cgacgtcggc
     5221 gtagtagacg gccatgcgga actggtcggg tagcgcgcac atggcttccc ggattttatt
     5281 gtcgcccaac ctctccaatg cgtgctgttc ggcggaccgt gttccagttg ttgactcatg
     5341 aatggcagtg acctgcaact cctcggtgaa gtggccgggc atgtagtgca ccgggcgccg
     5401 ccgttccttg cggtaggtgg tgatatacgc gttcctcatg atccggaaca gccagccgcg
     5461 aaggttgttg tcctgttgga acgtatgcag gcccgcgtag gccttcatca gggtgtcctg
     5521 gacgaggtct tcggcgtcag cgtgattgcg cgtcatgcgc attgcctggc ggtagagcgg
     5581 ctcacgcagt gggatcacgt cagattggta ccgggcgatc agctcgccac cgtccgcgcc
     5641 gcatgcatgc gtgtcgggat cgacagtgct gcgtgccgat tcggcaacag ggctatgcaa
     5701 ctgtgctgtc atcgggacac caacacatag ccggcgatcc cgaacgccac cggcagcatt
     5761 gccagcattc cattgattgc gctcagcatc gttcactctt ttccgtgtgc tcatctgact
     5821 cacggctaca acggtcctcg cgttcacggc agagcactat cgaccgagga ttgatgttta
     5881 gcgccttgtg gatgtagtgc tagccgggca cgtgacgttg cactcacgtc aacgagcccc
     5941 ggacccaaat accaagggtc cggggctcgt ctaggcgaca ggcggcctca ggccgaaacg
     6001 tcggctgctg cccacttttt cagttgtgct tcgagggcct ccggcttgga aacgaggccg
     6061 acgacgccgc cgccgtcccc gcgatcctcg atgccgaagt tgggcaccag attgcgaatg
     6121 ccaccttcgc gcgccaactt tgagggatag tgcggcacct cgggtgcctc gtcgccgacc
     6181 gggtccgcat cccacccgat ggtgtcgggg tgagtgcccg gccacggggg cgttcgcagc
     6241 gccatcgacg gcgtgtggct gaacccgacc ggtggagtga accgtgtgaa cgtgccccac
     6301 gggctgtcct ggtccacccc gtactcgatc aggtcctgca cagtgaccgg cgcatagggt
     6361 tcgtcgtcga ccggcccgac gagctggttg cccagcatcg aagtgcgcat gagcgacgcg
     6421 tcgacgtgcc agaagccgcc tttctcttcc cgctcggcca gtgccgccac caccgcaatg
     6481 gccagcagat agccggtgat gtggtcgttc atcaggcccc cggtgaccgt gggagcgtcc
     6541 agcccgagac cctcggaatg cacgtgcatg gtgccggtga ccgcctgcgc aatctgctca
     6601 aatccgcggc gcccggccca cggcgtacca ggaggggcga acgactcgga cccgtaaacg
     6661 aggttgggat tgatttcccg cagctcggcc tcgctgagac caagacgatc gagtgcaccg
     6721 gggcgctggc tgctgatcag cacgtccgcg ctggccagca aatcgatgaa ccgcttcttg
     6781 ccgtcgggac tcttgatgtc ggtcaggatg gacttcttgc cccagttgac gctcatccag
     6841 attggggtca cccagtcgcc gtggggcgaa tggaccagga tgacgtccgc gccctgctcg
     6901 gccaacgcga aacccgcagt gggaccggcg acgacgttgg tcaggtcgac cacccgcacg
     6961 ccttccagcg ggcggaattt ggcggttccc agcgcgcggt tcttcgtcgt gccctgttgg
     7021 tggatgccga acacgggcag cttggccgtc gcttgtccct gcgggtgggc gagccattcc
     7081 tgcggggtac gcaccatgcc cagcgccaga ttcagcccgg cgaactcatc ctcgagttcc
     7141 tgcgcggtgt acttctcgac cgcggcctgg atcgcctccg gggtgtccgc gcactgcagt
     7201 cgggccgtca gccccttggc caagtgcggg aaaagcccga tcatctgcac ataacggcca
     7261 tcctttgtgc ggtaggtccc gttgtcggcg gccatcggcc aggtgtccag cgtgagtgcg
     7321 tagccgttga ggaattgggt ctgcagctcg ttgagatgaa acccgcacag ccggcggttc
     7381 agggtcatgg tctgggccgg caggccgcga atgcggccga gatgttcgac gaccgagccg
     7441 aatgcggcca tgacgccgac ggcgtagtcg tgcaacttga ttgggctcgg caggtaggac
     7501 acccccggct ccacggtgat cttcgacgga tcaatcgcga acggaagcgc ggctgcgacc
     7561 tcgccgtagg ccttctcctg gatgggatgg ctgaacatcg gatggttctc cttcattggt
     7621 cgaccggagc agccgctgaa tccgaactgc ggtgctgtcg gtcgaaaaag tgattggttg
     7681 tcgacctcca cggtggtcgc aggggcagcg aagaaccatc gaccacggat tgatgtttag
     7741 ggccgtgtgg atgtaccgct atacgagccc ggctagccag gcgtcagtca gcccctcgca
     7801 tgagcggcgc caattgttct cgggaggtga tgcccagctt ggcgaacgcg ttgtggacat
     7861 gggttttcac cgtatgaacg gataggtgca gctgggtcgc gacgtcgcgg tttgtcgcgc
     7921 cccccgcgat taggttcaca actctgagct cggagtcggt gagactatcc cagcccgttc
     7981 ttgcccgctg ttggccgacg atgcggcgct gtacgccgag cgtgcgcagc tggcggccga
     8041 cgcggcgtgc atcggcgagc gcttcgtggc gcgtgtaggt gtcgaatgcg gcattgagct
     8101 gactgagagc ctcatcaccg cggccgatgc gcgcgagttc gtcgccggcg tcctcggcgg
     8161 ctgcggcgta aagtagtggc cgcgaggacg attgaagcga ctcggcggca gccaccaaaa
     8221 ctgcggcatc gcgttcgagg atgccgcgga catacgctgc gatgccggag aagagcgggt
     8281 tcgcgggccg ctcgcgttcg agcaggtcag tgacctgcag gacacgtgcg cgaaggccgg
     8341 cgtcgccgcc ggcagacgca atccgcgcac agaagaccgc ccgatctagg gtcgccggcc
     8401 cgactggtgg cccgaccagg ctgacgtcgc cgaaccagcg catcgcgtca tggacgtcgc
     8461 cgcgctgcca tgccgccagc gccagcacgt gcgccgctgt ccgacccaga agagatgagc
     8521 gagcggagta ggcctcgcgc gcgctattta ccatctgctg caacagggtt cggtcatcgg
     8581 tgcgcgccgc cacctcagcc agaatcatca cacggagcat gtctgtgtcg gtcggcgccg
     8641 gctctgcggg aggcaagaat tcaaccgcgg ttcgggccgc agacaaccgg cccgcagcca
     8701 ggtggacgag cccggcatcg acggcccata tatttacggc cattgcgttg ccttcctggc
     8761 ggacttgcgc tgtgccactg gcgatctggg ccgtcgcctc gtccacccgg ccgacagccg
     8821 ccagcaggag cgcgtagtgc tgtgcggcca ggtcacgagc tgcggtcgca tagctcgtgc
     8881 gggcgagcgc acaagccttc tcgagggggt gtagggcccg tcctggatac ccgtcagcag
     8941 cgtcgagcaa ggccagggtg acgtcggcca ggattgtggc ctcgagatcc cccgttccct
     9001 cggcagcctc ggcagcctcg tctgctgccg ggcgtcgttc ccctgtttgg tcgtgcaacg
     9061 ccaggttgta ggccagccag gccagatgcc gcgcccgagt gacctcgttg atctcgccca
     9121 actgcagcgc tcggcgattc tcctccacgc gccgttgggt gctgtgctta gtgagcgtca
     9181 tgacgcgcaa gcggatctcg gcctccgctt cgggcgatgc cgcctccgag agcgccgcga
     9241 cggccaattc ttccgcctcc tcgtagcgcc tggcccggtt gagcagctcc accgtttcag
     9301 cgaccagtcg tccatgctga gggtcttggc cgggcaacag ttccaacgcg cgcttgctca
     9361 agtccgccgc cgtacccgca tcgctgcgac taaccgatcg ggcggcctgc cgcagcgcac
     9421 tgacagcctc ccggtcgccc ggttcggcgc tgcgcgccag ctgagtggcc acctcggccg
     9481 gcgccgcgcc catacccagc atgatcgacg cgcattggcg ctccatcgcg cggcgcagcg
     9541 actgcggaat tgactgccgc gtggcttcac gcagtaagtc gtgccgaaac ctcagccgct
     9601 caccgtcttc cacgagcaga tccgcgcgca ccgcctcctc gagccccgac agcaacgaca
     9661 ccggcgggcg gtcgagcatc gcggccaaca gcccagccga gaaccggtcg ggtaacagcg
     9721 cggctacccg caccatctcg ccggcatcgt tggacagcag gtccagccgc tgctgcatgg
     9781 tagcgctcag acgacgcggc agtccgtctc cggcggccac cgcacgcccg ccgctgacag
     9841 tcagccgacc ctcctcgccc aaacccccca cgagttcgct gaccagaaaa ggatttccgt
     9901 gcgcctttgc cgccagagtc agcagcgacg cgtcagcatt ggcgcggacg gtgtcgcaga
     9961 ccatgtcggc gaccgcgtcc ggcgccatcg cgccaacccg cacaaaagtc g
//
LOCUS       DASRJA010000634         6691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2198559,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000634 DASRJA010000000
VERSION     DASRJA010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198559"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFC01_28385"
     CDS             <1..284
                     /locus_tag="VFC01_28385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZN83570.1"
                     /translation="AIDGVQIIGPTTMQDRGSPVAFVVDGVHAHDVGQVLDDDGVAVR
                     VGHHCAWPLHRRFGIAATARASFAVYNTHDEVDRLVAGVRHAVEFFGRS"
     gene            285..755
                     /locus_tag="VFC01_28390"
     CDS             285..755
                     /locus_tag="VFC01_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HZN83571.1"
                     /translation="MRLEQLYQEVILDHYKHPHHRGLREPFHAQSFQVNPTCGDEVTL
                     RVALSDDGETVTDISYDGQGCSISQASTSVLTDQVIGQSVGDALKTVAAFTEMVSSRG
                     TVEGDEDVIGDGIAFAGVAKYPARVKCALLGWMAFKDALAQASEETPDERHRSA"
     gene            733..1080
                     /locus_tag="VFC01_28395"
     CDS             733..1080
                     /locus_tag="VFC01_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HZN83572.1"
                     /translation="MSDTAVPNDELLADLEEAMRDVVDPELGINVVDLGLVYDMNVEK
                     GEQGNVALIDMTLTSAACPLTDVIEDQSRTALIGSGLVNELKINWVWNPPWGPDKITE
                     DGREQLRALGFTV"
     gene            complement(1090..1371)
                     /locus_tag="VFC01_28400"
     CDS             complement(1090..1371)
                     /locus_tag="VFC01_28400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83573.1"
                     /translation="MIKKLAAATGVAASLAAVSIAIAQAAPNSHGTDNANDHAQERGI
                     SVAGVAGNGPAAVIGAVQGFAPEAAQNGLANAASHVPAAPTTTTEAAAP"
     gene            1616..2191
                     /locus_tag="VFC01_28405"
     CDS             1616..2191
                     /locus_tag="VFC01_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83574.1"
                     /translation="MVTVPASVWRGGFFRRALTLSAALGIPLGALAWIDSGLLLAGIL
                     TVFITGLIYGIWLPRRMARYWPGAKELSGDERVAVVRTVRAGERIGDPRLAQAVVDYN
                     VGMHAAAEQARPFRWIVPLVLVVGVATTVYDAVFGSWGNVVASFIYLVALLIELFWWP
                     KKRDEFLANGDRAADLATQILEKQDSERTDL"
     gene            2206..3087
                     /locus_tag="VFC01_28410"
     CDS             2206..3087
                     /locus_tag="VFC01_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZN83575.1"
                     /translation="MTAKMVAQVRRFNRTVTQRIGVLNENFLASNRSLGQNRLLWEIG
                     VDGCDIRSLRERLDLDSGYVSRLLRALETGGHVVVEPSPTDGRVRIARLTKTGRGELA
                     ALDRRSDDAAAAILQPLNDRQRTKLVTAMAEVERLLIASTVQVRECDPRHRDARFCLK
                     TYYDELAVRFPAGYDPAISPVADAEMTPPAGLLLVASLHGEPVGCGALIFYPDGVGLV
                     KRMWVAPSVRGLGLGRRLLLELEDRGRDHGVQLMRLETKDVLTEAIRMYQTSGYREVA
                     PFNDEPYADHWFEKALS"
     gene            complement(3080..3811)
                     /locus_tag="VFC01_28415"
     CDS             complement(3080..3811)
                     /locus_tag="VFC01_28415"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZN83576.1"
                     /translation="MAGVDRRTVLAAIAAGVVAGVARPPAAGAVRLVVPLARVPAPVG
                     VLIRLPGDGNQLALTVDDGASSPVVAAFGQFCRDTGTRLTFFVNGANASWTDNAAALR
                     PMVDSGQLQMGNHTWSHPYLNRISLTAVHGQIQRNADFLRNTYGTDGTPYFRPPFGVH
                     TGDTDRVAADLGYTTVTLWSGTIGDSLPESEANLVAAATRSFAPQQIVLAHANLPTIT
                     HCYDQLSQLIASRNLQTVTLSDVFS"
     gene            3963..4595
                     /locus_tag="VFC01_28420"
     CDS             3963..4595
                     /locus_tag="VFC01_28420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83577.1"
                     /translation="MSDGFDEVYVDTRCQLRGVAESLIAGPQYRASGTIRLAVRPNGF
                     AGVSFPLAVHGTELVWPDGAAPLAGPVDALAAAAGVAAAPPDGVYEIVDPLPTDAFLR
                     LDTAAADWIHRSHYAGGHAIKKVLPEAHPVLGPEHFDVAAAEDEVNYGVSAGDDYHPT
                     PYAYVAPWTARIGPFWNAPFGAVYPLDPAHDVDALALDIADFFQRGRKEL"
     gene            4595..5311
                     /locus_tag="VFC01_28425"
     CDS             4595..5311
                     /locus_tag="VFC01_28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZN83578.1"
                     /translation="MTAFNSDFSGLRALFINCTLKRSPEISNTQGLVDISTGIMQKHG
                     VEVEVIRAVDHDIATGVWPDMREHGWATDDWPAIFDKVLAADILVLAGPIWLGDNSSV
                     MKQVHERLYGGSHLLNDAGQYLYYGRVGGCLITGNEDGVKHCAQNVLYTLQHIGYTIP
                     PQADAGWIGEAGPGPSYLDPGSGGPENDFTNRNTTFMTWNLMHLARMLKSNGGVPAHG
                     NIRSGWDAGCRYDFANPEYR"
     gene            5330..5962
                     /locus_tag="VFC01_28430"
     CDS             5330..5962
                     /locus_tag="VFC01_28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN83579.1"
                     /translation="MTETVDESTLIAALRDGDEATFAQLIDQHTPSMLRVARGYVPSR
                     EIAEEVVQETWIALLKGIDNFEGRSSLRTWLFAVMINIAKRRGLRERRDADAAVAAFT
                     GGTVDPARFRTAGDPYPGHWKQGEEPSPFPDTPEGSVLGNELVDVARRELDKLPERQR
                     IVVTLRDMLGFDSSEVCELLDVSLANQRVLLHRGRAAVRQVLEDYLKDAS"
     gene            5959..6201
                     /locus_tag="VFC01_28435"
     CDS             5959..6201
                     /locus_tag="VFC01_28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZN83580.1"
                     /translation="MTGRDAMDCNELVELVTAYLDGSLDLATRARFDMHLLDCDGCEN
                     YLQQFRETVQTVGKIRDDELDPAFRSRLLDAFKDFR"
     gene            6271..6639
                     /gene="trxA"
                     /locus_tag="VFC01_28440"
     CDS             6271..6639
                     /gene="trxA"
                     /locus_tag="VFC01_28440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZN83581.1"
                     /translation="MATRDITAEEFNDTITDNDIVLVDFWASWCGPCKQFAPTFAASS
                     EEHPDVVYAKVDTEAEQQLAAAADIRSIPTLMVFKKGKLVFNQAGALPPAALEELVQR
                     VKEFDIDAAIAAQNNGQKPE"
BASE COUNT         1205 a         2142 c         2168 g         1176 t
ORIGIN      
        1 tcgccatcga cggggtgcag atcatcgggc cgacgacgat gcaggaccgg ggatcgccgg
       61 tggccttcgt ggtcgacggg gtccatgccc acgatgtggg gcaggtgctc gacgacgacg
      121 gcgtggccgt gcgggtcgga catcactgcg cctggcccct gcaccgccgt ttcggcatcg
      181 ccgcgacggc ccgcgcctcg ttcgcggtgt acaacacgca cgacgaggtc gaccgactgg
      241 tggccggcgt ccggcacgct gtcgaattct ttggcaggtc gtaagtgcgg ctggagcagc
      301 tctatcagga agtgatcctc gatcactaca agcacccgca tcaccggggg ctgcgtgagc
      361 cgtttcacgc gcagtcgttt caggtgaatc cgacgtgcgg cgatgaagtc acgctccggg
      421 tcgcattgag cgacgacggc gaaacagtga ccgacatttc ctatgacggg cagggctgct
      481 cgatcagcca ggcctcgacg tcggtgctga ccgaccaggt gatcggccaa agtgtaggcg
      541 atgcgctcaa gaccgtcgcg gcgttcaccg agatggtgtc ctcgcgcggc acggtcgagg
      601 gcgacgagga tgtaatcgga gacggcatcg cgttcgccgg tgtcgcgaag tacccggcgc
      661 gggtgaaatg cgcgctgctg ggctggatgg ctttcaagga cgcccttgcg caagccagtg
      721 aggagacgcc agatgagcga caccgcagtg cctaacgacg aactgctcgc cgacctcgag
      781 gaggcgatgc gcgacgtcgt cgaccctgaa ctcgggatca acgtcgtcga cctcggcctc
      841 gtctacgaca tgaatgtgga aaaaggggag cagggcaatg tcgcgctgat cgacatgacg
      901 ctgacctcgg cggcctgtcc gctcactgac gttatcgagg atcaatcgcg caccgcgttg
      961 atcggcagcg ggctcgtcaa cgagttgaag atcaactggg tgtggaaccc gccgtggggc
     1021 cccgacaaga tcaccgagga tggccgcgaa cagttgcgcg cgctcggatt taccgtctag
     1081 cggctttggt tagggcgccg ctgcctcggt cgtggtggtc ggcgcggcag gcacatgcga
     1141 cgccgcattg gccaacccgt tctgcgcagc ctccggcgcg aacccctgga ctgcgccgat
     1201 cactgctgcc gggccattac ccgccacgcc cgcaacgctg atgccccgtt cctgggcgtg
     1261 gtcgttcgcg ttatcagtgc cgtgggaatt cggtgcggcc tgagcgatgg cgatcgagac
     1321 tgcggcgaga ctcgcagcga cccccgtggc tgctgcaagc ttcttgatca tgtaatcgaa
     1381 gcccttccgt tgaaccaatc cgggtacgtg cctcgctatg ccggtgaggt tgagccagcc
     1441 gcaagacgca ttcacacaat gatcttgaaa ttcgttgcta tttaccatct ttcaagcgct
     1501 taacctcggc ccacagcaaa cgcagccggc aacgtcccct gggttcggcg gggattgcgc
     1561 tctgggtatt gcagtttgcc cacctctgag cgacaactgt cgtcacaatc ggactgtggt
     1621 gacggtcccg gcgtctgtgt ggcgaggcgg tttttttcgt cgcgcgctga cgctcagcgc
     1681 cgctctcgga attcctttgg gcgctctggc gtggattgat tccggactcc tgctggctgg
     1741 cattctcaca gtcttcatca ccggactcat ctacggcatc tggttacccc ggcgaatggc
     1801 acggtactgg ccaggcgcca aggaactgtc cggcgacgaa cgcgtggccg tggttcgcac
     1861 cgtccgagcc ggcgagcgca tcggcgatcc ccggctggcc caggcggtcg tcgactacaa
     1921 cgtaggcatg cacgcggcgg ctgaacaggc ccggccgttt cgatggatag tgcccctcgt
     1981 actcgtcgtc ggcgtcgcga cgactgtcta tgacgctgta ttcggctcat ggggcaacgt
     2041 cgtggcctcg tttatttatc tggtggcgct attgatcgag ttgttctggt ggccaaagaa
     2101 gcgcgacgag ttcctggcca acggcgatcg cgcggcggac ctcgccacgc agatcttgga
     2161 aaaacaggat tcagaacgca ccgatctctg actaaggtca gagatatgac cgcgaagatg
     2221 gtggcgcagg tccgacggtt caaccgcacc gttacgcagc gcatcggcgt gctcaacgag
     2281 aacttcctgg ccagcaaccg ctcgcttggt cagaaccgct tgctctggga gatcggggtc
     2341 gacggctgcg acatccgctc actgcgggaa cgcctcgacc tcgactccgg ctacgtgagc
     2401 cggctgctgc gggcgctgga aaccggcgga cacgtcgtcg tcgaaccgag cccgacggac
     2461 gggcgcgtcc gcatcgctcg actcaccaag accggacggg gcgaactcgc ggcattggac
     2521 cggcgctccg acgacgccgc cgcagcgatc ctgcaaccgc tcaacgatcg gcagcgcacg
     2581 aaactggtca cggccatggc cgaagtggaa cgcctgctga tcgcctccac ggttcaggtc
     2641 cgtgagtgcg atccgcgcca ccgagacgct cggttctgcc tcaagaccta ctacgacgaa
     2701 ctcgccgttc gcttcccggc tggctatgac cccgcgatca gcccggtcgc ggacgccgaa
     2761 atgacaccgc ccgcagggct tttgcttgtc gcgtccctac acggcgagcc cgtcggctgc
     2821 ggtgcgttga tcttctatcc cgacggcgtc ggcctggtga agcggatgtg ggtggcgccc
     2881 tccgttcgcg ggctggggct cggccgccgt ctgctgctcg aactcgagga ccgcggtcgc
     2941 gaccacggcg ttcaactgat gcggctggag accaaggacg tgttgaccga agcgatccgg
     3001 atgtatcaga cgtccgggta tcgcgaagtg gcgccgttca acgacgagcc gtacgccgac
     3061 cattggttcg aaaaggcgct cagctgaaca cgtcggacag tgtcacggtc tgcagattgc
     3121 ggctggcgat gagctggctc agttggtcgt agcagtgggt gatggtcggc aggttggcgt
     3181 gcgccaacac aatctgctgc ggcgcgaacg agcgtgtcgc ggccgcgacg aggttggcct
     3241 cgctttccgg gagcgaatcg ccgatggtgc cgctccataa cgtgacggtc gtgtatccca
     3301 gatcggcggc cactcgatcg gtgtcgccgg tgtgcacgcc aaagggcggc cggaaatagg
     3361 gagtgccgtc ggtgccatac gtgttgcgca ggaagtcggc gttgcgctgg atttggccgt
     3421 ggactgcagt caggctgatc cggttgaggt acggatgaga ccaggtgtgg ttgcccatct
     3481 gaagctggcc ggagtccacc atgggccgca gcgccgcggc gttgtccgtc cacgacgcgt
     3541 tggcgccgtt gacgaagaag gtcagccggg ttccggtgtc acggcagaac tggccgaacg
     3601 ctgcgacgac cggactgctg gcgccgtcgt ccaccgtgag ggccaactgg tttccgtcgc
     3661 ccggcagcct tatcagcacg ccgacgggcg ccggcacccg ggccagcgga accaccagcc
     3721 ggacagcgcc tgccgccggt ggcctggcga cacccgcgac gactccggcc gcgatcgcgg
     3781 ccaacacggt gcgccgatca acccccgcca cattgcaatg ataatccgtg cctgttaaat
     3841 cgcttctgcg cctttgcttc tcgtttactg agaagcggct gtacagtttc gcggccgcaa
     3901 tgggtcgatg gcgtgtaacg ctaggacccg atccgggcac tacagggcgg gaaggagtca
     3961 gaatgtccga tggtttcgac gaggtctacg tcgatacccg ctgccagctg cgcggagtag
     4021 ccgagagcct gatcgccgga ccgcagtacc gtgcgtcggg caccatccgc cttgccgtgc
     4081 gacccaacgg cttcgcgggt gtttcctttc cgctcgcggt gcacggcacc gagctggtat
     4141 ggcccgacgg cgccgcaccg ctagctggtc cggtcgacgc gctcgccgcc gccgccggcg
     4201 ttgcggccgc gccgcccgac ggcgtgtacg agatcgtcga cccgctgccg accgacgcct
     4261 tcttaaggct cgacaccgcc gcggcggact ggatccaccg cagtcactac gcaggcgggc
     4321 acgcgatcaa gaaggttctg cccgaggcgc acccggtgct cgggcctgag cacttcgacg
     4381 tcgcggccgc cgaggacgag gtcaactacg gcgtctccgc cggcgacgat taccacccga
     4441 ccccctacgc atacgtcgcg ccatggactg cgcgcatcgg cccgttctgg aacgcgccat
     4501 tcggggcggt gtacccgctg gaccccgcac acgatgtcga cgcgcttgcg ttggacatcg
     4561 ccgacttctt ccaacgcggt cgaaaggaat tgtgatgaca gccttcaaca gcgacttcag
     4621 cggccttcgg gccctgttca tcaactgcac gttgaagcgg tcgcccgaga tcagcaacac
     4681 ccagggactc gtcgacatca gcaccggaat catgcagaag catggcgtcg aggtggaggt
     4741 catccgcgcc gtcgaccacg acatcgccac cggggtgtgg ccggacatgc gcgagcacgg
     4801 ctgggccacc gacgactggc ccgccatctt cgacaaggtg ctggcggccg acattctggt
     4861 gctcgcaggc ccgatctggt tgggggacaa cagctctgtg atgaagcagg tgcacgagcg
     4921 gctgtacggg ggatcgcact tgctgaacga cgccggccag tatctgtact acggccgggt
     4981 cggtggatgt ttgattacag gcaacgagga cggcgtcaaa cactgtgcgc agaacgtgct
     5041 ctacacgttg cagcacatcg gctacacgat cccgccacag gccgacgcgg ggtggatcgg
     5101 cgaagccggg ccaggaccgt cgtacctgga tcccggatcc ggcgggccgg agaacgactt
     5161 caccaaccgg aacaccacct tcatgacgtg gaatctgatg catctagccc ggatgttgaa
     5221 gtcaaacgga ggcgttcccg cccacggaaa catccgatca ggctgggatg ccggatgtcg
     5281 ctacgacttt gccaaccccg aataccggtg agcggctacc gtgactgtgg tgaccgaaac
     5341 agtcgatgaa tcgacgttga tcgccgcgct gcgtgacggc gatgaggcca cgttcgccca
     5401 actcatcgac cagcacaccc catccatgct gcgggtggcc cgcggatatg tacccagccg
     5461 ggagatcgcc gaggaggtcg tacaggaaac ttggatcgcg ctgctgaagg gcatcgacaa
     5521 cttcgaaggc cgatcctctt tgcgcacttg gcttttcgcg gtgatgatca acatcgccaa
     5581 acggcgcggg ctacgcgaac gccgcgatgc cgacgcggcg gtcgcggcgt tcaccggcgg
     5641 cacggtcgac ccggcgcgct tccgaaccgc aggcgacccc tacccgggac attggaaaca
     5701 aggcgaggaa ccctcaccgt tcccggacac ccccgagggc tcagtcctcg gcaatgaact
     5761 ggtcgatgtc gcccgccgcg aactcgacaa gttgccggaa cgtcaacgga tcgtcgtcac
     5821 gctgcgcgac atgctcggat tcgactccag cgaggtctgc gaactgctcg acgtcagcct
     5881 tgccaaccaa cgggtgttgt tgcaccgcgg cagggccgcg gttcgccagg tgctcgaaga
     5941 ctatttgaag gacgcctcat gacaggccgc gacgcaatgg actgcaacga acttgtcgag
     6001 ctcgtcaccg cctacctcga cgggtcactc gacctggcca cccgcgcccg cttcgacatg
     6061 cacttgctcg actgcgacgg ctgcgagaac tacctgcagc agttccgcga aaccgtacaa
     6121 acggtgggca agatccgcga cgacgagctg gatccggcgt tccgcagccg cctgttggac
     6181 gcgttcaagg acttccgctg atccccgccg ggcagtttgc actgccaata tggtgggaac
     6241 atcctgattt accacagcgt taggaagatc gtggctacgc gagacatcac cgccgaagag
     6301 ttcaacgaca ccatcacgga caacgacatc gtgctggtgg atttctgggc gtcgtggtgc
     6361 ggcccatgta agcaattcgc gccgaccttc gcggcctcgt cggaggagca ccctgacgtc
     6421 gtctacgcga aggtcgacac cgaggccgag cagcagctcg ccgcggctgc cgatatccgc
     6481 tcgatcccga ctttgatggt cttcaagaag ggcaagctgg tgttcaacca ggctggtgcc
     6541 ctgcctccgg cggcgctcga agagctcgtg cagcgcgtca aggagttcga catcgacgcc
     6601 gcgatcgccg cccagaacaa cggccagaag ccggagtaaa tcgcacgcga tagcgtgcac
     6661 gggtgaactt cgtcctagta gataagccgc g
//
LOCUS       DASRJA010000635        11294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1207828,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000635 DASRJA010000000
VERSION     DASRJA010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11294
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207828"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="VFC01_28445"
     CDS             <1..157
                     /locus_tag="VFC01_28445"
                     /inference="COORDINATES: protein motif:HMM:NF037234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZN83582.1"
                     /translation="PGDPLSVVRATASAVWNAQLDGRRVVLMAGDDTARQAMDRWSMI
                     SPPNRLA"
     gene            166..351
                     /locus_tag="VFC01_28450"
     CDS             166..351
                     /locus_tag="VFC01_28450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83583.1"
                     /translation="MSTDPDQIRAQIAELLADVPDPAQEGSTFDELAESDIDTIAARL
                     EEAHDLLVRALESVEKG"
     gene            358..1167
                     /locus_tag="VFC01_28455"
     CDS             358..1167
                     /locus_tag="VFC01_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HZN83584.1"
                     /translation="MTRRARVDAELVRRGLARSRQQAAELIGAGRVSIDGMPAAKPAT
                     AISISAALTVERDDGRSWVSRGAHKLMGALDVFGLAIDTRRCLDAGASTGGFTEVLLD
                     RGAREVVAVDVGYGQLAWSLRNDPRVVVLERTNVRDLTLDAIDGPVDLVVADLSFISL
                     ATVLPALTACASDDADIVPMVKPQFEVGKDRVGAGGVVSDPGLRADAVLTVARRAAEL
                     RWHTVGVTASPLPGPSGNVEYFLHLRASTESPLQGVQLEQAVRQAVEEGPQ"
     gene            1164..2087
                     /locus_tag="VFC01_28460"
     CDS             1164..2087
                     /locus_tag="VFC01_28460"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HZN83585.1"
                     /translation="MTCERHILLVVHTGRDEATEVARRVEKVLGDNGIGLRVLSAEAV
                     DRGPVHLAPDDMRALGVEIEVVDADERAAEGCELVLVLGGDGTFLRAAELARNVEIPV
                     LGVNLGRIGFLAEAEADHIDTVLEHVIKRDYRVEERMTLDVVVRVDGQTVDRGWALNE
                     ASLEKGPRLGVLGAVLEVDGRPVSSFGCDGVLVATPTGSTAWAFSAGGPIVWPDLEAI
                     LVVPNNAHALFARPMVTSPNAAIAIEIEADGHAALVFCDGRREMIVPAGGRLEVTRCG
                     TPLKWVRLDSAPFTDRLVRKFRLPVTGWRGQ"
     gene            2087..3838
                     /gene="recN"
                     /locus_tag="VFC01_28465"
     CDS             2087..3838
                     /gene="recN"
                     /locus_tag="VFC01_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZN83586.1"
                     /translation="MLSEIRIESLGAISTATAEFDRGFTVLTGETGTGKTMVVTGLHL
                     LGGARADATRVRSGSDRAVVEGRFTTTELGEGVAERVDEILESSGADRDDDGSVIAAR
                     SVSRDGPSRAYLGGRSVPAKSLGTFTTELLALHGQNDQLRLMRPEEQRAALDRFADVE
                     SHLKRYRRVRDDWLEARHDLTDRKQCAREMAQEADRLKFGLNEIDTVAPEPGEDDALV
                     ADIRRLSELDALREAAQTARAALSGLDDDPSDMSATNGIGHAKSALEGTDDSALRALA
                     EQLAGALAVITDVSTELGDYLAELPSDASTLETKLARQGELRTLTRKYAADIDGVLQW
                     AHESRERLAQLDVSEEALAELERRVDELEAKVVSAATELTKSRTKAAKGLAKAVTAEL
                     AGLAMADAGFTVSVDHLSARADDSAPLTLPSGATVHAGHDGVDAVEFGFAAHRGTDML
                     PLSKSASGGELSRVMLALEVVLAASAEGTTMVFDEVDAGVGGRAAVQIGRRLARLARS
                     HQVIVVTHLPQVAAYADVHLVVDSGGRNNSSGVRRLDDDDRVAELARMLAGLGESDSG
                     RAHARELLDAAQNDRAD"
     gene            3970..5154
                     /gene="steA"
                     /locus_tag="VFC01_28470"
     CDS             3970..5154
                     /gene="steA"
                     /locus_tag="VFC01_28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HZN83587.1"
                     /translation="MKMSALLSRTANSRPGITGTARVDRDIDRLLRRVTPGDIVVIDA
                     LDLDRITADALVEANVAAVVNASPSISGRYPNLGPEVLVANGVTLIDETGPEVFKKVK
                     DGARVRLHEGGVYVGDRRLILGTERTDHEIHELMHEAKSGLVAHLEAFAGNTIEFIRS
                     ESPLLIDGIGIPDIDVDVNRRHVIIVADDHDATIDLKALKPFIKEYQPVLVGVGSGAD
                     VLRKAGYRPQLIVGDPEKISTETLRCGAQVVLPADADGHAAGLERIQDLGVGAMTFPA
                     AGSAADLALLLCDHHGASLIVTAGHTASIEEFFDRSRQQSNPSTFLTRLKVGEKLVDA
                     KAVATLYRSRVSGGAIAMLVLAMLIAVIVALWVSRTDVAVLDWITDYWNRFSLWVQGW
                     VS"
     gene            5165..6106
                     /locus_tag="VFC01_28475"
     CDS             5165..6106
                     /locus_tag="VFC01_28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HZN83588.1"
                     /translation="MISLRSHAISLAAVFLALAVGVVLGSGLLSNTLLSGLRDDKHEL
                     QTQINTLTDEKNALNEKLNAAGEFDAQMAPRILHDTLTGKSVVVFRTPDANDDDVDAL
                     SRMVGQAGGTVTGTVALTQEFVDANSAEKLLSVVNSPIVPAGKQLNTASVDQGSQAGD
                     LLGIAVLINKDSKAQPVDDAQRDTVLAALRDTGFITYGNQRIGPANTALIVTGGGLGD
                     DAGNRGATVARFAAGMAPHGSGTVLVGRDGSASGTAAVAVTRSDGGLSAAVSTVDDVD
                     AESGRLTAVLALGDVVNGRRPAQYGIGQGASSVTLPQ"
     gene            6273..8036
                     /locus_tag="VFC01_28480"
     CDS             6273..8036
                     /locus_tag="VFC01_28480"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZN83589.1"
                     /translation="MPALRKHPQTATKHLFVTGGVVSSLGKGLTASSLGQLLTARGLQ
                     VTMQKLDPYLNVDPGTMNPFQHGEVFVTEDGAETDLDVGHYERFLDRNLSGSANVTTG
                     QVYSSVIAKERRGEYLGDTVQVIPHITDEIKSRILAMAAPDSDGNRPDVVITEIGGTV
                     GDIESLPFLEAARQVRHEVGRENCFFLHCSLVPYMAPSGELKTKPTQHSVAALRSIGI
                     TPDALILRCDRDVPEPLKNKIALMCDVDIDGVISTPDAPSIYDIPKVLHREELDAYVV
                     RRLNLPFRDVDWTQWNDLLERVHDPQETVRIALVGKYIDLSDAYLSVAEALRAGGFKH
                     RAKVEMRWVASDDCETETGAAAALSDVHGVLIPGGFGIRGIEGKIGAISYARKRGLPV
                     LGLCLGLQCIVIEAARSVGLIAANSAEFDPKTPDPVISTMADQRDAVAGEADLGGTMR
                     LGAYPAVLEPNSIVAQAYDATEVSERHRHRYEVNNEYRDRIAESGLKFSGTSPDGHLV
                     EFVEYAADVHPFIVGTQAHPELKSRPTRPHPLFVAFIGASLEYKAEERLPGMDIPEQR
                     ANGVERPDNVAQLLQEPATRG"
     gene            8029..8649
                     /locus_tag="VFC01_28485"
     CDS             8029..8649
                     /locus_tag="VFC01_28485"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZN83590.1"
                     /translation="MAEHDFDTVDSETLYVGKIFALRVDDVRMPHGNVAKREVIEHYG
                     AVAVLALDDDKNVVMVYQYRHPVGRRLWELPAGLLDLGGEPPHITAARELIEEAGLEA
                     TEWRVLVDLVSAPGFSDESVRVYLATGITDVGRPDAHDEEADLQVKWFSLEEARRMVL
                     DGKIVNSIAIAGILAAHDVEDPSSLRTVDAPWIDRSKAFAARQAHK"
     gene            8646..9593
                     /gene="xerD"
                     /locus_tag="VFC01_28490"
     CDS             8646..9593
                     /gene="xerD"
                     /locus_tag="VFC01_28490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HZN83591.1"
                     /translation="MTTFRSALDDQLQGYLDHLTIERGVAANTLSSYRRDLRRYAEHL
                     TTRDIDDLSKVTETDVSDFLVSLRRGDPDTKTAPLSAVSAARAVVAVRGLHRFAASEG
                     LTEVDVAREVKPPTPSRRLPKSLTIDEVLALLDAAGGDSEADGPLTLRNRALLELLYS
                     TGARISEAVGLDVDDIDTHARSVLLRGKGGKQRLVPIGRPAVTALDAYLVRGRPELAR
                     RGRGTPAIFLNARGGRLSRQSAWQVLQDSAERAGIKAAVSPHVLRHSFATHLLDGGAD
                     VRVVQELLGHASVTTTQIYTMVTVNALREVWAGAHPRAR"
     gene            10140..11264
                     /locus_tag="VFC01_28495"
     CDS             10140..11264
                     /locus_tag="VFC01_28495"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN83592.1"
                     /translation="MGEHEVAVIGAGPAGIATAISLQERGVRPLVIDRASQMVASWRA
                     RYDRLKLNTGRQFSHLPGRRYPKGTPTFPTRDQVIEHFEGHAREADVQVRLNTTVHRI
                     DPGDGGGWRLGASTGDIDARHVVVATGLMHTPFMPDWLNRGGFGGEVLHSSQYRNTET
                     FRDKRVLVVGSGSSGMEIAHDLATGGAAKVWMAVRTPPNIMLRGGPGGLPGEVISLPL
                     FHAPIRLADALALKARRRFIGDLSEFGLPLPDEGPFGRLARAGAVPSLVDLEVIDAIR
                     DRSIEVVAGVQGFDGESVRLVDGRGLKPDAVICATGYHNGLDPMVGHLGVLDERGEPR
                     AGWDRPAARGLWFIGFSRRPALIGYVGKQSRRLAKKIANT"
BASE COUNT         1952 a         3682 c         3799 g         1861 t
ORIGIN      
        1 cccgggcgac ccgctatcgg tggtccgcgc caccgccagt gcggtctgga acgcccagct
       61 cgacgggcgg cgcgttgtcc tgatggccgg tgacgacacc gcgcgacagg ccatggaccg
      121 ctggtccatg atcagcccgc caaatcggct agcgtgaaca gcgagatgag taccgacccg
      181 gaccagatcc gcgcccagat tgcagaactg ctggcagatg tgcccgaccc cgcgcaggag
      241 ggctccacct tcgacgagct cgccgaatct gacatcgaca ccatcgcggc gcgacttgag
      301 gaggcccacg acctgctggt gcgtgcgctg gaatcggtcg agaagggcta accgcacgtg
      361 acgcggcgcg ctcgtgttga cgccgagctg gtgcggcgcg gtcttgcgcg ctcgcggcag
      421 caggccgccg agttgatcgg ggcgggccgc gtcagcatcg acggcatgcc cgcggctaaa
      481 cccgcaacag ccatctcgat cagcgccgcc ctcacggtag agcgcgatga cgggcggtcc
      541 tgggtgtccc gtggtgcgca taagttgatg ggcgccctcg acgtgttcgg cctcgcgatc
      601 gacacccgtc gctgcctgga cgcaggagct tccaccggag gcttcaccga ggtgctgctc
      661 gaccggggcg cccgcgaggt cgtcgccgtc gacgtcggct acggccagct ggcgtggtca
      721 ctgcgcaacg acccgcgggt ggtggtgctc gaacgcacca acgtccgtga tttgacgctc
      781 gacgcgatcg acggacccgt tgacctggtg gtcgccgatc tgtcgttcat ctcgttggcg
      841 acggtgctgc ccgcgctgac tgcctgcgcg tcagacgacg cggatatcgt tcctatggtg
      901 aagccacagt ttgaggtcgg taaagatcga gtcggcgcag gtggagtcgt gtcggacccc
      961 gggctgcgcg cagatgccgt gcttaccgtc gcgcggcgcg ccgctgaact ccggtggcac
     1021 accgtcggtg tcaccgccag cccgttgcca ggcccgtccg gaaacgtcga gtactttctg
     1081 cacctgcgcg ccagtactga gagcccgctg cagggcgtac agcttgagca ggcagtacgc
     1141 caggccgtcg aagagggccc gcaatgacgt gcgaacgcca catcctgctg gtggtgcaca
     1201 cggggcgcga cgaggccacc gaggtcgctc gtcgagtgga gaaagtgctg ggcgacaacg
     1261 gcattgggct gcgcgtgctc tccgccgaag ccgtcgaccg cgggccggtg cacctggcgc
     1321 ccgacgacat gcgtgcgctc ggtgtcgaga tcgaagtggt cgacgccgac gagcgcgctg
     1381 ccgaaggctg cgaactcgtc ctcgtcctcg gcggcgacgg aacgttcctg cgtgctgccg
     1441 agctggcccg caacgtcgaa ataccggtgt tgggcgtcaa tctcggccgc atcgggtttc
     1501 ttgccgaggc cgaggccgac cacatcgata cggtgctcga gcacgtcatc aagcgcgact
     1561 accgcgtcga ggagcgcatg acgcttgacg tggtggtacg cgtggacggc caaaccgtgg
     1621 accgcggttg ggcgctgaac gaagccagcc tggagaaagg accccgcctc ggggtgctgg
     1681 gggcggtgct cgaggtcgac ggtcgaccag tctcgtcgtt cggctgcgac ggggtgttgg
     1741 tggcgacgcc gaccgggtcg accgcgtggg cgttctccgc aggcgggccg atcgtctggc
     1801 ccgatctgga agcaattctg gtggtgccca acaacgctca cgcgttgttc gcgcggccga
     1861 tggtgacgag tccgaacgcc gctatcgcca tcgagatcga ggcggatggt catgccgcgc
     1921 tggtgttctg cgacgggcgc cgcgagatga tcgtgcctgc gggcggccgg ttggaggtga
     1981 cgcgctgcgg caccccgctc aagtgggtgc gcctggacag cgcaccgttc accgatcgac
     2041 tcgtgcgcaa gttccggttg ccggtcacgg ggtggcgcgg acagtagtgt tatccgaaat
     2101 ccgtatcgag tccctcggcg ccatcagcac cgcgaccgcc gagttcgatc gcgggttcac
     2161 cgtgttgacc ggcgagacgg gcacgggcaa gacgatggtc gtcaccggtc tacacctgct
     2221 tggcggcgcc cgcgccgacg ccacccgggt gcgctccggc tccgatcgtg cggtagtcga
     2281 aggacgtttc accacaacgg aactcggcga gggcgtggcc gagcgcgtcg acgagatcct
     2341 cgagtcttcg ggagcggacc gcgacgacga cggcagcgtc atcgccgcac gatctgtcag
     2401 ccgtgacggg ccgtcgcggg cctacctcgg tggacgtagt gtgcccgcaa aatcgctggg
     2461 cacgttcacc accgagctgc tcgcgttgca cggccagaac gatcaactcc ggctgatgcg
     2521 accggaggaa caacgggcgg ccctggaccg ctttgccgat gtcgagtcgc acctgaagcg
     2581 gtatcggcgg gtgcgcgacg actggctcga ggcacgccac gatctgaccg accgcaaaca
     2641 atgcgcgcgg gaaatggccc aagaggccga ccggctcaag ttcggactca acgagatcga
     2701 caccgtcgca ccggaaccgg gtgaggacga tgcgctcgtc gccgacatcc gccggctgtc
     2761 ggaactcgac gcgctgcgcg aggccgcgca aaccgcacgc gcggcgctct ccggcctgga
     2821 cgacgatcca tcggacatgt cggccaccaa cggcatcgga cacgcgaagt cggcgttgga
     2881 gggcaccgat gactcggccc tgcgggcgct ggcggagcag ctcgccggcg ctttggccgt
     2941 catcaccgac gtgtcgaccg aactcggcga ctatctggcc gaattgccca gcgacgccag
     3001 cactttggag accaagctgg ctcggcaggg cgagctgcgg acgctcaccc gcaaatacgc
     3061 tgcggacatc gacggtgtgc tgcagtgggc ccacgaatcg cgggaacgcc tcgctcagct
     3121 cgacgtctcc gaggaggcgc tggccgaact cgagcgacgt gtcgacgaac tcgaggccaa
     3181 ggtcgtcagc gccgcaacgg aactcaccaa gtcgcggacc aaggccgcga agggtctggc
     3241 gaaagcggtg acggccgaac tcgccgggct ggccatggcc gacgccgggt tcacggtgtc
     3301 ggtcgaccac ctttcggccc gagccgacga ctccgccccg ctcaccttgc cctcgggcgc
     3361 gacggtccac gccggccatg atggcgtcga cgctgtcgag ttcggcttcg ccgcgcaccg
     3421 cggaaccgac atgttgcccc tcagcaaaag cgcctcgggc ggtgaactgt cccgggtcat
     3481 gctggcgctg gaggtggtgc tggccgcttc ggccgagggc accacgatgg tcttcgacga
     3541 agtcgacgcc ggggtcggag gtcgggccgc ggtgcagatc ggacggcggt tggcccggtt
     3601 ggcgcgctca caccaggtca ttgtcgtcac gcatctgccg caagtggccg cctatgccga
     3661 cgtccacctc gtcgtcgaca gcggcggccg gaacaactcc agcggggtgc gccgcctcga
     3721 cgacgacgac cgcgtcgccg aactggcacg catgttggcc gggctggggg agtccgacag
     3781 cggccgggcg cacgcccgcg agctgctcga cgcagcgcaa aacgaccgcg cagattaggt
     3841 caccgacgcg atcgccctac cagcacagac gtgaggcatc gtgttgcgaa tgtgacaaaa
     3901 gggacttctg gggctgatgt tacggcgcgc ctccctgcga tggcaggcga tcctcgacag
     3961 aatcggccca tgaagatgtc agcgctgcta tcccgtactg ccaactcacg gccgggcatc
     4021 accggcaccg cccgggttga ccgcgacatc gatcggctgc tgcgacgtgt cactcccggt
     4081 gacatcgtcg tcattgacgc tctggatctg gaccggatca ccgcggacgc gctcgtcgag
     4141 gccaacgtcg ccgcggtcgt caacgcgtcg ccgtcgattt ccggccggta ccccaacctc
     4201 ggacccgagg tgctcgtcgc caacggcgtc acgctgatcg acgagacggg cccagaggtc
     4261 ttcaagaagg tcaaagacgg ggcgcgggtg cgcctccacg aaggcggcgt gtacgtcggc
     4321 gaccgtcggc tgatcttggg caccgaacga accgaccacg agatccacga gttgatgcac
     4381 gaggccaaga gcggcctggt ggcccacctt gaggcgttcg cgggcaacac catcgagttc
     4441 attcgcagtg agagcccgct gttgatcgac gggatcggca tccccgacat cgatgtcgac
     4501 gtcaaccgca ggcacgtgat catcgtcgcc gacgaccacg acgccaccat cgacctcaag
     4561 gcgctcaagc cgttcatcaa ggaatatcaa cccgtattgg tcggtgtcgg cagtggcgcc
     4621 gacgtgctcc gcaaggcggg gtaccggccg cagctcatcg tcggcgaccc cgagaagatc
     4681 agcaccgaga ccctgcgatg cggtgcccag gtggtactgc ccgccgacgc cgacggccac
     4741 gccgcaggcc tcgagcgcat tcaggatctg ggagtcggtg cgatgacatt tcccgccgcg
     4801 ggctcggcag ccgacctggc gctactgctg tgcgatcacc acggcgcgtc gctgatcgtc
     4861 accgccggcc acaccgccag catcgaggag ttcttcgatc ggtcacggca gcagagcaac
     4921 ccgtcgacat tcctgacccg gctcaaggtc ggcgagaaac tggtcgacgc caaagccgtt
     4981 gcgacgctgt atcgcagccg ggtgtccggc ggcgccatcg caatgcttgt gctcgcgatg
     5041 ctgatcgcgg tgatcgtcgc attgtgggtg tcgcgcaccg acgtggcggt gctcgactgg
     5101 atcaccgatt actggaaccg attctcgctg tgggttcagg gctgggtctc ctagcgggtc
     5161 cccaatgatt tcgctacgct cacacgcgat ttcgctcgcc gcggtgttcc tggcactcgc
     5221 cgtcggcgtc gtgctcggtt cgggcctgct gtccaacacg ctgctgtccg gtctgcgtga
     5281 cgacaaacac gagctgcaga cccagatcaa cacactcacc gacgaaaaga acgcgctgaa
     5341 cgaaaagctc aacgctgcag gtgaattcga tgcgcagatg gcgccgcgga tcctgcacga
     5401 cacgctgacg ggcaaatctg tggtggtgtt ccgcaccccc gacgccaacg acgacgacgt
     5461 cgacgcactg tcgcggatgg tggggcaggc aggtgggacg gtcaccggca cagtggcgtt
     5521 gacgcaggag ttcgtcgacg ccaactccgc ggaaaaactg ctgtcggtgg tgaactcacc
     5581 gatcgtgccg gccggaaaac agctgaacac cgcgtcggtc gaccagggtt cgcaggcggg
     5641 cgatcttctc ggaattgcgg tgctgatcaa caaggattcg aaggctcaac cggtcgacga
     5701 cgcccagcgc gacaccgtgc tggccgcgct gcgcgacacc ggcttcatca cgtacggcaa
     5761 ccagcgcatc gggcccgcca acaccgcgct gatcgtcacc ggcgggggac tgggtgatga
     5821 cgccggaaac cgcggtgcca cggttgcacg gttcgcggcg ggcatggccc cgcacggctc
     5881 cggcacggtc ctcgtgggcc gggacggctc tgcctccggc accgctgcgg tcgcggtgac
     5941 aaggtccgac ggcgggttga gcgctgcggt cagcaccgtc gacgacgtcg acgccgagtc
     6001 cggccggctc acagccgtgc tggccctggg cgacgtcgtc aacggtcgcc ggccggcgca
     6061 gtacggcatc ggccagggcg cctcatcggt aacgctgccg caataggccc ctcgctacgc
     6121 gtgggccccc gagcacgcgg gggcccaccg agtggcgtgt cagcgacggc ttgtcggcgt
     6181 cggtgttaag gtggggttcc gtgggtcggc aggccccaaa caggtcccag cctggaaagt
     6241 atcagccctg cccgtcgtca cggaggttgc tcttgccagc gctacgcaag cacccgcaaa
     6301 ccgccaccaa gcacctcttc gtaacgggcg gcgtggtttc ttcactcggc aagggcctga
     6361 cggcgtcgag cctcggccag ctgctgacgg cgcggggcct gcaggtgacg atgcagaagc
     6421 tggaccccta cctcaacgtc gaccccggca ccatgaaccc gtttcaacac ggcgaggtgt
     6481 tcgtcaccga agacggcgcc gagaccgacc tcgacgtcgg gcactacgag cgtttccttg
     6541 accgaaacct gtccggctcg gccaacgtca ccacgggaca agtgtattcg tcggtgatcg
     6601 ccaaggagcg ccgcggcgag tacctcggcg acaccgtcca ggtcatcccg catatcaccg
     6661 acgagatcaa gagccgcatc cttgcgatgg ccgcacccga ctccgacggc aatcggcccg
     6721 acgtggtgat caccgaaatc ggcggcacgg tcggtgatat cgagtcgctg cccttcctcg
     6781 aagcggcgcg ccaggtccga cacgaggtcg gccgcgagaa ttgcttcttc ctgcactgct
     6841 cgctggtgcc gtacatggcg ccctccggtg agctcaagac caagcccacc cagcactcgg
     6901 tggccgcgct gcgcagcatc ggtataacgc cggatgcgct gatcctgcga tgcgatcgcg
     6961 acgtgcccga accgctgaag aacaagatcg cgctgatgtg cgacgtcgac atcgacggcg
     7021 tgatatcgac tccggacgcg ccgtcgatct acgacattcc caaggtgctg caccgcgagg
     7081 agctggacgc ctacgtggtg cgccggctga atctgccgtt ccgcgacgtc gactggaccc
     7141 aatggaacga cctgctcgag cgcgtgcacg atccccagga gactgtgcga atcgctctgg
     7201 tgggcaagta tattgacctc tcggatgcat atctgtcggt cgccgaggca ctgcgggccg
     7261 gtggcttcaa acaccgcgcc aaggtcgaga tgcgttgggt ggcctcggat gactgcgaaa
     7321 ccgaaaccgg tgccgctgcc gcactgagcg atgtgcatgg tgtgctgatt cccggcggct
     7381 tcgggattcg cggcattgag gggaagatcg gcgccatctc ctacgcccgc aagcggggat
     7441 tgccggtgct cggcctgtgc cttggattgc agtgcatcgt catcgaggcg gcccgttcgg
     7501 tgggactgat tgccgcgaac tccgcagagt tcgatccgaa gacgccggat cccgtgatct
     7561 ccacgatggc tgatcaacgc gacgcggtcg caggtgaggc cgacctgggc ggcaccatgc
     7621 ggctgggcgc ttatccggcc gtgctggagc cgaattccat tgtggcccag gcgtatgacg
     7681 cgacggaggt atccgagcgg caccgtcacc gctacgaggt gaacaacgaa tatcgcgacc
     7741 gcatcgccga aagcgggttg aagttctctg gcacgtcgcc ggacgggcat ctggtcgagt
     7801 tcgtcgaata cgccgccgat gtccatccct tcatcgtcgg cacgcaagcg catccggagc
     7861 tcaagagccg gcccacccgc ccgcatccgc tgttcgtcgc gttcatcggc gcgtcgctgg
     7921 aatacaaggc cgaggaacga ttgccgggca tggatatccc ggagcagcgc gcgaatggcg
     7981 tagagcgtcc cgacaatgtc gcgcaactgc tgcaagaacc cgcgacccgt ggctgaacac
     8041 gacttcgata cggtcgattc cgaaactctc tacgtcggaa agattttcgc ccttcgcgtt
     8101 gacgacgtgc ggatgcccca cggcaacgtc gcgaagcggg aagtgatcga gcactacggc
     8161 gccgttgccg tcctcgcttt ggacgatgac aagaacgtcg tcatggtcta tcaataccgc
     8221 catcccgtcg gtcgacggtt gtgggaactg cccgcgggct tgctcgacct cggcggtgaa
     8281 ccgccgcaca tcaccgctgc gcgggaactc atagaagagg ctggactgga ggccaccgaa
     8341 tggcgggtgc tggtcgattt ggtgtcggcg ccaggcttca gcgacgagag tgtgcgggta
     8401 tacctcgcga ccggcatcac cgatgtcgga cgccccgacg cccacgacga ggaagccgac
     8461 ctgcaagtga agtggttttc tctcgaagag gccaggcgca tggtgcttga cgggaaaatc
     8521 gtcaattcca ttgcgatagc cggcattctg gccgcacacg acgtcgagga tccgtcatcg
     8581 ctacgcaccg tcgacgcccc gtggattgat aggtccaagg ccttcgccgc tcggcaggcc
     8641 cacaaatgac gaccttccgc tcagccctcg acgaccagct gcagggttac ctggaccacc
     8701 tgaccatcga acgcggcgtg gcggcgaaca ccttgagttc ctaccgccgg gacctgcgtc
     8761 gctacgccga acacctgaca acgcgggaca tcgacgatct gtccaaggtc accgaaaccg
     8821 atgtgagcga cttcctggtg tcactgcgcc gcggcgatcc agacacgaaa accgcaccgc
     8881 tgtccgcggt ttcggcggcc cgcgccgtgg tcgccgtgcg gggcctgcac cgcttcgcgg
     8941 catccgaggg cctcacggag gtcgacgtgg cgcgggaggt caagccgccg acgccgagcc
     9001 gtcggctgcc caagagtctg accatcgacg aggtgctcgc gttgttggat gctgccggcg
     9061 gcgacagcga ggcggatggc ccgctgacgc tacgcaaccg tgcgctgctg gagctgctgt
     9121 attcgacggg cgcccggatc tccgaggcgg tcgggctcga cgtcgacgac atcgacaccc
     9181 acgcccggtc ggtgctgttg cgcggcaagg gcggtaagca gcggctggtg ccgatcggcc
     9241 ggcccgcggt cacggcgctc gacgcgtacc tggtgcgcgg ccgccccgag ttggcccgcc
     9301 gcggcagggg gacgccggcg atcttcctca acgcgcgcgg cggtcggctg tcgcggcaga
     9361 gcgcctggca agtactgcag gactccgccg aacgggctgg catcaaggca gcggtgtcgc
     9421 cacacgtgct acggcactcg ttcgccaccc acctgctcga tggcggcgcc gacgtccgcg
     9481 tggtccagga gctgctcggg cacgcatcag tgacgacgac gcagatctac accatggtca
     9541 cggtgaacgc gcttcgcgag gtctgggcgg gtgcgcatcc gcgggcccga tagctcctag
     9601 atcgctacgt gggcttctgc gcgcggggcg gccgggacca agaggcgctg agccccgacg
     9661 tagccctcta catcctcccg agtgacgaat cccagccgga cctggagatc ggtgaagctg
     9721 aggtggaacc gatccgcgat tttatggagc tcgtcagcgt tgggatagtc gtcttgcctg
     9781 atccggcggt aatacgtgct actggagacc ttgagcgcct gatagatctc ctggttcgtg
     9841 gatccggccg tccagaaggt aattcagcag cgccataagg tgccgcccgt tttcgtcggt
     9901 gcgaggcact cgcccacgat atacccagct caatagctat gtcatcgggg gcgtcaattt
     9961 tggataacaa aaaatgattc tccacatatc aatgattttt caaaaaagaa ctggttctgt
    10021 ccgtcatacg gattacaacg agattgctct gatattgaaa gcgatgcgac tgcgatcggc
    10081 atggcgtttg ctctgggcaa cgctgaacga tccgacggtc gggatcgttg gacgaggata
    10141 tgggcgagca tgaggtagca gtcatcggtg cgggcccggc cggaatcgcg acggcgatca
    10201 gtctgcagga gcgtggcgtt cgcccgttgg tcatcgaccg tgcatcccag atggtcgcgt
    10261 cgtggcgtgc tcgatatgac cgattgaagc tcaacaccgg ccggcagttc tcgcatttgc
    10321 cgggccggcg ctaccccaag ggcaccccga ccttcccaac ccgcgatcag gtcatcgaac
    10381 acttcgaagg gcatgcccgc gaagctgatg tacaggtgcg gctgaacacc acagtgcaca
    10441 ggatcgaccc aggggacggc ggcggttggc ggctgggcgc atcgacggga gacatcgacg
    10501 cgcgccacgt cgtcgtcgca accgggctca tgcacacgcc cttcatgccg gactggctca
    10561 atcgtggcgg cttcggtggt gaggttctgc actcgtcgca ataccgcaat accgagacgt
    10621 ttcgggacaa gcgggtattg gtcgtcggtt ccggctcgtc agggatggag atcgcccatg
    10681 atctggcgac gggaggggcg gccaaagtgt ggatggcggt ccgcaccccg ccgaacatca
    10741 tgctgcgggg cggccctggc gggctgcctg gcgaggtcat ctccctgccg ctgtttcacg
    10801 cgccgatccg gttggcagac gcgctcgcgt tgaaggctcg gcgccgattc atcggcgatc
    10861 tcagtgaatt cggtttgccg cttcccgacg agggcccgtt cggtcggctg gcccgggccg
    10921 gagccgtgcc gtcactcgtt gacctcgaag tgatcgacgc gatccgcgac cggtcgatcg
    10981 aagtggtggc aggtgtccag ggctttgacg gcgagagcgt ccggctggtg gacggccgtg
    11041 ggctcaagcc cgacgcggtg atttgtgcga ccggctatca caacgggctg gacccgatgg
    11101 tcggtcacct cggcgtgctc gacgaacgtg gggaaccgcg cgccggatgg gatcgaccgg
    11161 ccgcccgcgg actgtggttc atcggcttca gcaggcgccc ggcgctgatc ggctatgtgg
    11221 gcaagcagtc acgtcggctt gccaagaaga tcgcgaatac ctagccgcgg tactccgact
    11281 ccagcacgag gtcc
//
LOCUS       DASRJA010000636         4386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2337204,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000636 DASRJA010000000
VERSION     DASRJA010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2337204"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..2212
                     /gene="uvrC"
                     /locus_tag="VFC01_28500"
     CDS             230..2212
                     /gene="uvrC"
                     /locus_tag="VFC01_28500"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZN83593.1"
                     /translation="MPDPATYRPAPGSIPVQPGVYRFRDPHGRVIYVGKAKSLRSRLT
                     SYFADISSLAPRTRQMVMTAGSVEWTVVNTEVEALQLEYNWIKEFDPRFNIRYRDDKS
                     YPVLAVTLNEEYPRLMVYRGARRKGVRYFGPYSHAWAIRETLDLLTRVFPARTCSAGV
                     FKRHRQIGRPCLLGYIDKCSAPCIERVTAEEHRQIVNDFCDFLAGKTDRLARDMEHQM
                     NQASAELDFERAARLRDNISALKRALEKQAVVFGDGTDADVVAFADDELEAAVQVFHV
                     RGGRVRGQRGWIVEKSGEPGESSQEHLVEQFLTQFYGDQVELDGAADESLSPVPRQVL
                     VPCLPANADQLETWLSQLRGSRVSLRVPRRGDKRALAETVKRNAQDALNQHKLKRAGD
                     FTARTAALQSIQDALGLAEAPLRIECVDISHVMGTDVVASLVVFEDGLPRKSDYRHYA
                     IKDAAGDGRSDDVASIAEVTRRRFYRHLHDTQHPTELAPEGKSRKFAYPPNLYVVDGG
                     APQVNAAQAALDDLGVSDVAVIGLAKRLEEVWVPSEPDPVIMPRNSEGLYLLQRVRDE
                     AHRFAIAYHRSKRSKRMTASALDSVRGLGEHRRKALVTHFGSLTRLKQASVEEITAVP
                     GIGVATARAVLDALGVAPDSEAPEPVIGNDQTRASG"
     gene            2224..3123
                     /gene="rapZ"
                     /locus_tag="VFC01_28505"
     CDS             2224..3123
                     /gene="rapZ"
                     /locus_tag="VFC01_28505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZN83594.1"
                     /translation="MHEELRDGAGDGESGIDVVLVTGLSGAGRGTAAKVLEDLGWYVA
                     DNLPPELIAQMVELGLAAGSRITQLAVVMDARSRGFTGDLDVVRNDLATRNISPRVLF
                     LEASDDILVRRYEQNRRSHPLQGNQTLAEGIAAERQMLAPIRAAADLVIDTSTLSVPA
                     LRESIERAFGGETIAQTSVTVESFGYKYGLPMDADTVMDVRFLPNPHWVDELRPHTGQ
                     HPAVRDYVLGQPGAAEFLDTYHQLLDVVIDGYRREGKRYMTVAIGCTGGKHRSVAMAE
                     ALAARLQDDELTVRVLHRDLGRE"
     gene            3120..4145
                     /gene="yvcK"
                     /locus_tag="VFC01_28510"
     CDS             3120..4145
                     /gene="yvcK"
                     /locus_tag="VFC01_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HZN83595.1"
                     /translation="MSARIVALGGGHGLYATLSAARRLTPHVTAVVTVADDGGSSGRL
                     RSELDVVPPGDLRMALAALASDSPHGRLWATIFQHRFGGSGALAGHPIGNLMLAGLNE
                     VLADPVAALDELGRILGVKGRVLPMCPMGLSIEADVSGLESDPRMFRVIRGQVAIATT
                     VGQVRRVRLLPADPPATRQAVDAIMSADLVVLGPGSWFTSVIPHVLVPQLVAALQATT
                     ARRALVLNLVAEPGETAGFSVERHIHVLAQHAPGFTVHDIIVDSSRVPGEREREQLRR
                     TANILEARVEFADVSRPGTPLHDPARLAAALEDVRVRGLTSTPNIQEPTVPTPTVNGP
                     RGDDPWR"
     gene            4142..>4386
                     /gene="whiA"
                     /locus_tag="VFC01_28515"
     CDS             4142..>4386
                     /gene="whiA"
                     /locus_tag="VFC01_28515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HZN83596.1"
                     /translation="MTAEVKDELSRLLVNSVSARRAEVASLLRFAGGLHIVSGRVVVE
                     AEVDLGIIARRLRKDIYDLYGYNAVVHVLSASGIRKST"
BASE COUNT          752 a         1338 c         1563 g          733 t
ORIGIN      
        1 cgcacagtgg agggagcagg ggtcggcatc gttgaacccc ctgtcgccaa gcggccagga
       61 tgcgagccgg tacggaccga tcgtagggaa acatccgcag ttccgccgca cggggggcgg
      121 ccgtcggcgg cgacggcgaa ctggtccagc tctgcttttt ggcacgtcgg gggaagtact
      181 cgtggaagct acgcaagtcg ggtcaccggt cggcagtaac ctggaatccg tgcccgatcc
      241 agcgacgtac cggcctgctc ccgggtcgat tcccgtccag ccgggcgtct accggttccg
      301 tgatccgcac ggccgggtga tctatgtcgg caaggccaag agcctccgca gccggctgac
      361 gtcctacttt gccgacatct ccagcctggc gccgcgcacc cggcaaatgg tgatgacggc
      421 gggcagcgtc gagtggacgg tggtcaacac cgaagtcgag gcgctgcagc tggaatacaa
      481 ctggatcaag gagttcgacc cgcggttcaa tatccgctac cgcgacgaca agtcgtatcc
      541 cgtcttggcg gtgaccttga acgaggagta tccgcggttg atggtgtacc gcggggcgcg
      601 acgcaagggc gtccgctact tcgggccgta ttcgcatgcg tgggccatcc gggaaaccct
      661 cgacctgttg acccgggtgt tcccggcacg cacgtgttcc gcgggagttt tcaagcggca
      721 taggcagatc gggcggccgt gcctgctggg ctacatcgac aaatgttcgg cgccttgtat
      781 cgaacgagtc accgctgagg agcaccggca gatcgtcaat gacttctgcg atttcctcgc
      841 cggcaagacg gaccggttgg cccgcgacat ggaacaccag atgaaccagg catccgcgga
      901 gctggacttc gagcgggcgg cacggttgcg cgacaacatt tccgccctca agcgcgcgtt
      961 ggagaaacag gccgtggtgt tcggtgacgg caccgacgcc gacgtcgtcg cgttcgccga
     1021 cgacgaactc gaagcggctg tgcaggtgtt ccatgtccgc ggcggccggg tgcgcggcca
     1081 gcgcgggtgg atcgtcgaaa aatcaggtga gccaggcgaa tccagtcagg agcatctcgt
     1141 cgagcagttc ctgacacagt tttacggcga tcaggtcgag cttgacggcg ccgccgacga
     1201 gtcgctgagc ccggtacccc ggcaggtact cgtgccatgt ctgccggcca atgccgacca
     1261 gctcgagacc tggctgtctc agctgcgagg gtcccgggtg tcgctgaggg tgccgcgccg
     1321 cggcgacaag cgcgcacttg ccgaaacggt gaagcgtaac gcccaggatg cactgaacca
     1381 gcacaaactc aagcgcgccg gggacttcac cgcgagaaca gctgcgctgc aaagcattca
     1441 ggatgcacta ggtttggcgg aagcgccgct acgcatcgag tgtgtggaca tcagccacgt
     1501 catgggcacc gatgtggtgg cgtcgttggt ggtgttcgag gacgggctgc cgcgcaaatc
     1561 cgactaccgc cactacgcga tcaaagatgc cgcgggggac ggccgctcgg acgatgtcgc
     1621 atcgatcgcc gaggtgacgc ggcgacggtt ctaccggcac ctgcacgaca cccagcaccc
     1681 gactgagctt gccccggaag ggaaatcgcg taagttcgcc tacccgccaa acttgtacgt
     1741 cgtcgatggc ggcgcaccgc aagtcaacgc ggcccaggcg gccctcgacg atctcggggt
     1801 gagcgacgtc gcggtcatcg ggctggccaa gcggttggag gaggtgtggg tgccgtcgga
     1861 gccggatccc gtcatcatgc cgcgcaacag cgaggggctt tacctgctgc agcgggttcg
     1921 tgacgaagca caccggttcg cgatcgccta ccaccgcagc aagcgctcga agcggatgac
     1981 ggcgtcggca ctggactcgg tccgcggact gggggagcac cggcgcaagg cgctggtcac
     2041 gcacttcggc tcgctgacgc ggttgaagca ggccagcgtc gaggagatca ccgcggtgcc
     2101 cggcatcggc gtggcaacag ccagggcggt gctggacgcg ctcggcgtag cgcccgattc
     2161 cgaagcgccg gagcccgtta tcggcaatga tcagaccagg gcatcaggat gacgaaccag
     2221 ggcatgcacg aggaattgcg cgacggcgcc ggcgatggtg aatcgggcat tgacgtggtc
     2281 ctggtcacgg ggttgtctgg cgcggggcga ggcaccgcgg cgaaggtact ggaggacctc
     2341 ggctggtatg tcgcagacaa cctgccaccc gagctgatcg cccagatggt cgagctgggg
     2401 ctcgcggcag gctcccgcat cacccagttg gcggtggtga tggatgcgcg gtcgcggggt
     2461 ttcaccggcg atctagacgt ggtgcgaaac gatctggcca cccgcaacat cagcccacgg
     2521 gtgttatttc tcgaggcgtc cgacgacatc ctggtgcgcc gctacgagca gaaccggcgc
     2581 agtcatccgt tgcagggcaa ccagactctg gccgaaggca ttgccgcgga acggcagatg
     2641 ctggcgccga tacgcgccgc ggcggacctg gtgatcgaca cgtcgacgct gtccgtaccg
     2701 gcgctgcgcg aaagcatcga gcgggcattc ggcggcgaga cgattgcgca gaccagtgtc
     2761 accgtcgagt cgtttgggta taagtacggg ttaccgatgg acgccgacac ggtgatggat
     2821 gtgcggttcc tgccgaatcc gcattgggtg gatgagctgc gtccgcacac cggtcagcac
     2881 ccggcggtgc gcgactacgt gctcggtcag ccgggcgccg cggagttctt ggacacctac
     2941 catcagctgc tggacgtcgt gatcgacggc tatcggcggg aagggaagcg ctacatgacc
     3001 gttgccatcg gctgcaccgg cggcaagcac cgcagtgtcg cgatggccga ggcactggcg
     3061 gcgcgactac aggacgatga gctcacggta cgggtattgc accgggatct gggccgcgaa
     3121 tgagcgcacg gatcgtcgcg ctcggtggcg gacacgggct gtatgccacg ttgtcggcgg
     3181 cccgtcggct caccccgcat gtcacggcgg tggtcaccgt cgccgacgac ggcggatcct
     3241 cggggcggct gcgcagcgag ctggatgtag tgccgccggg tgatctacga atggcgttgg
     3301 cggcgttagc atctgacagc ccacatggcc ggctgtgggc gaccatcttt cagcaccggt
     3361 tcggcggaag cggagcgctg gctggccatc ccatcggcaa tctgatgctg gcggggctca
     3421 acgaggtgct ggccgatccg gtggcggcgc tcgacgagct gggccgcatc ctcggggtca
     3481 agggcagggt gttgccgatg tgcccgatgg ggttgagcat cgaggccgac gtgtcgggtc
     3541 tcgagtcgga tccgcgaatg ttccgggtga tccgtgggca ggtggcgatc gccaccaccg
     3601 tcgggcaggt gcggcgggtg cggctgttgc ctgcggaccc gcccgccacg aggcaggccg
     3661 tggacgcgat catgtcggcc gacctggtgg tgttgggccc cggttcgtgg tttaccagcg
     3721 tgattccgca cgtgctggtg ccacagctgg tcgcggctct gcaggcgacg acggcgcggc
     3781 gcgcgttggt gctgaacctg gtggccgagc cgggggagac ggctggcttc tcggtggaac
     3841 ggcacatcca tgtgctggcg caacacgcgc cggggttcac cgtgcacgac atcatcgtcg
     3901 actccagcag agtgcccggc gaacgcgaac gtgagcagtt gcgccgcacg gcgaacattc
     3961 tcgaggcccg tgttgagttt gctgacgtgt ccagacctgg tacaccttta catgacccgg
     4021 cgaggttggc cgcggctctg gaggatgtac gtgtgcgcgg gctgacgtcg acgcccaaca
     4081 tccaggagcc gaccgtgccg actccgacag tgaacggacc gagaggtgac gacccgtggc
     4141 gatgacggcc gaggtcaagg acgagctgag ccggcttctg gtcaactcgg tgagcgctcg
     4201 ccgcgccgag gtcgcctcct tgctgcgctt cgctggcggt cttcacatcg tgtcggggcg
     4261 tgtggtggtg gaggccgaag tcgacctcgg gatcattgcg aggcggctgc gtaaggacat
     4321 ctacgacctg tacggctaca acgcggttgt gcacgtgtta tcggccagcg gtatccgcaa
     4381 gagcac
//
LOCUS       DASRJA010000637         7002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_932172,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000637 DASRJA010000000
VERSION     DASRJA010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932172"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1033)
                     /locus_tag="VFC01_28520"
     CDS             complement(50..1033)
                     /locus_tag="VFC01_28520"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN83597.1"
                     /translation="MRRREFITLLGGVAAADALATLRRAGAQAQALRVGTCGTQPRAR
                     SFLQGFDVRMRELGHIEGTNYTMDYIDLQGRPDRYGDAMQQLVARKADVLVAFGPEDC
                     LKAALAATQTIPIVMVAIDFDPVSLGYVTSLARPTGNVTGIILEQIELAKKRLQLVKD
                     AFPAIGKAMAFWDRLSADQWHATRDNAATFGFDLAGIELTDYPYDYARALAQAPPDHR
                     GFLFVMTSPLFARDRDRIVQFTLLNRLPSMGVFREHVDQGGLASYGPSRTAMSRRTAD
                     YVHRIARGAKPGDLPIERPTIFELVINLRTAKALGLEFSQAMLLRADEVIE"
     gene            complement(1030..2004)
                     /locus_tag="VFC01_28525"
     CDS             complement(1030..2004)
                     /locus_tag="VFC01_28525"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN83598.1"
                     /translation="MKRREFITLLGGAAAWPFAARAQQSAMPVIGLLHSASSDGTTDQ
                     LRGFRLGLKETGYVEGENVAIVYRFAENCIDRLPELAAELVHRRVAVIATAGHDSAFA
                     AKAATTTIPRLFIAGTDPVELGLVASLARPGGNATGINFLGAELTAKRLELLREMVPG
                     AARVAVLVNPASPATIPLREVEAAARAMGLQLQVLNASTSREIDAAFATLMRERPDAL
                     FVGGDTFFNSRRVQLANLAVRHVVPATYTLRDFPEIGGLMSYGTNLSDSYRQVGVYAG
                     RILKGAKPADLPVVQATKFELVINLQTARMLGLSVPPTLLATADELIE"
     gene            2312..2779
                     /locus_tag="VFC01_28530"
     CDS             2312..2779
                     /locus_tag="VFC01_28530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83599.1"
                     /translation="MAKREQDPIVRALVPDPTAGPPSVAVLRGFLGDSTQPDHYRLYL
                     SRTLSSYVDIPADQILHTSQLPNDGGTRVWVPRTLELRFVRTVSTEVQARFLQGSIVN
                     RHLALGGASLAAEALRAQARRLRRVDEWFSLECGHSEEDHGATCGDTCPACEE"
     gene            2815..3924
                     /locus_tag="VFC01_28535"
     CDS             2815..3924
                     /locus_tag="VFC01_28535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HZN83600.1"
                     /translation="MTPLSAIDVPDYLLARGLVSTRSIVDSDLVVHELSGRNRTFVAE
                     CTNAASYVLKQGATPEGTAAAHEGEAYRALSATRSMGPHLPQFHGYDADARVLVVEYL
                     RDAEDLANYHRHRRRPPIRTASAVGAILADLHELPVAQTGQVGYRRQRPSVLSLHRPG
                     PRMLAESSRVTLDLIRIIQGTYGFGFHLDELNADWSATNLIHQDVRFKNFVRTTSSRG
                     IDRPRLKLIDWELACLGDPRWDIGSALGDYMSLWLTSIPVTGSAPTEQSAELARCPLD
                     GIQPAIRACWDVYVKRRRLGVSADALLPVVVRFAAARLVQTALEIAQDSANLTTDIVL
                     HLQAAYNVFERPGVAAAHMFGLTSDTIGRVGGEKR"
     gene            4269..4937
                     /locus_tag="VFC01_28540"
     CDS             4269..4937
                     /locus_tag="VFC01_28540"
                     /inference="COORDINATES: protein motif:HMM:NF037672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector HopA1 family protein"
                     /protein_id="HZN83601.1"
                     /translation="MVERSGLLVWIKYEQLCLTDGALPEPGVSVSIRQSKELPRRSPG
                     FYLALGNHGMKSRDVDPVVRLYWNVIAESAPQLVELVTTRLNAADIPFRLKTINDARR
                     YTRCDAAVLYGPKRCYPAIAEIVAGIHAELAASLVPGTPAFTKRLAPGLALAEDPGTG
                     DSFGMHRCRLFAEGILNAREDGRRSTSARRDSVIAAFAAAGITTERPYLNPGSADTYQ
                     AVSE"
     gene            4934..6268
                     /locus_tag="VFC01_28545"
     CDS             4934..6268
                     /locus_tag="VFC01_28545"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HZN83602.1"
                     /translation="MTVDYLGVARGIGARLADTAIWHEGRCNWLGVRVFGNGQAGFRE
                     TVAALGGDLYSGSSGVALFLAELHAVAGGDAFAGTAMGAIEHALGRADSAEDIGPGLY
                     DGRLGIALAAARIGMLVRDSQLLERAADVADRAHRLASELGFDLLSGRAGGIVALLIL
                     AKLLDDDGLMEHATKLGDELIATVTEDGRYHPRSDVRARRPLTGLSHGAAGVGYALGE
                     LFHNTGDARYLHAAEAAFAYERAHFDPETRNWPDFRHDTGRRRGQVPPVFTTQWCHGA
                     PGIGLTRLRAYRLRDDDSCLTEAVAAIATTRDAVAVSLAKPGANYSLCHGLSGNAEVL
                     LYAEESLGSEGAEHRALAIRVAEHGIERFAAPNRMWPCGTREGVTPNLMLGLAGIGHF
                     YLRLHDSTVPSVLLMRPESFVGRAVIDGRVGEDPQPGWQRGDPEQSASVTLR"
     gene            6534..6785
                     /locus_tag="VFC01_28550"
     CDS             6534..6785
                     /locus_tag="VFC01_28550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83603.1"
                     /translation="MCDGGGSPTAVAATLPRPAAGTAVDPIALEPVDEVVITTLVDNS
                     YDTLMGDTGPARRAPLPGVAALEIGIGADQYRLAHQDRE"
BASE COUNT         1185 a         2265 c         2297 g         1255 t
ORIGIN      
        1 cacgctgcca ccgccgcgcc gccgaggagc gtgatgaact cgcgccgtct cattcgatca
       61 cctcgtcggc acgcagcagc atggcctgcg aaaactccag gccgagcgcc ttcgcggtcc
      121 gcagattgat gacgagctcg aagatggtcg gccgctcgat cggtaggtcg cccggtttcg
      181 ctccccgcgc gatgcggtgg acgtagtcgg cggtccgccg cgacatcgcg gtgcgactcg
      241 gcccgtacga cgccagtccc ccctgatcga cgtgttcgcg gaacaccccc atcgacggca
      301 gccgattgag cagcgtgaac tgcacgatcc gatcgcggtc gcgcgcgaac agcggcgacg
      361 tcatgacgaa gagaaagccg cgatggtctg gcggggcctg cgccagggcc cgcgcgtagt
      421 cgtagggata gtcggtgagc tcgatgccgg cgagatcgaa tccgaacgtc gcggcgttgt
      481 cgcgggtcgc gtgccactgg tccgccgaca gacgatccca gaacgccata gccttgccga
      541 tcgccggaaa ggcatctttc accaattgca ggcgcttctt ggcgagctcg atctgctcga
      601 ggatgatgcc ggtgacgttg ccggtcggcc gcgccaggct ggtcacatag ccgagcgaga
      661 ccgggtcgaa gtcgatcgcg accatgacga tcggaatcgt ctgggtcgca gccagcgccg
      721 ccttcaggca atcctcgggc ccgaaagcaa cgaggacgtc cgccttgcgg gcgaccagtt
      781 gctgcatggc atcgccgtag cggtcgggcc tgccttgcag gtcgatatag tccatcgtgt
      841 aattcgtgcc ctcgatgtgt ccaagctccc tcatgcgcac gtcgaagccc tgaaggaacg
      901 atcgcgcgcg cggttgcgta ccgcaggtcc cgacccgcag cgcctgggcc tgcgcgccgg
      961 cccgccgcag cgtagcgagc gcgtctgccg ccgccacgcc gccaagaagg gtgatgaact
     1021 cacgccgcct cattcgatca gctcgtcggc ggtggcgagc agcgtcggcg gcaccgagag
     1081 gccgagcatc cttgcggtct ggaggttgat cacgagctcg aacttggtcg cctgtaccac
     1141 cggcaagtcc gccggtttcg cacccttgag gatgcgaccg gcatagacgc cgacttgacg
     1201 ataggaatcc gaaaggttgg ttccgtagct catcagcccg ccgatttcgg gaaagtcacg
     1261 caacgtatat gtcgcgggga ccacgtggcg caccgccaga ttggcaagtt gcacccgccg
     1321 actgttgaaa aaggtgtcgc cgccgacgaa gagggcgtcg ggccgctcgc gcatgagggt
     1381 tgcgaaggcc gcatcgatct cgcggctggt gctggcgttg agcacctgga gctgcagacc
     1441 catggcgcgg gcagccgctt ccacttcccg taaggggatc gtcgccggac tggccggatt
     1501 gacgagcacg gcgacgcggg ccgctccggg gaccatctcg cgcaggagtt ccagccgttt
     1561 tgccgtcaat tcggccccaa gaaaattgat acccgtcgcg ttgccgcccg gccttgcaag
     1621 gctcgcaacc agaccaagtt cgaccgggtc tgtgccggcg atgaacagcc tcggaatcgt
     1681 cgtcgtcgcc gccttggcag cgaacgccga atcgtggccg gccgtagcga tcacggcgac
     1741 ccgccggtgg accaattcgg ccgccagctc cggcaaccga tcgatacagt tctcggcgaa
     1801 acggtacacg atcgctacgt tctcgccctc gacataacca gtctctttca ggcccaggcg
     1861 aaagccgcgc agttggtcag tagtcccgtc agacgacgcg ctatgcagga gcccgatcac
     1921 cggcatcgcc gactgctgcg cgcgcgccgc gaacggccaa gccgccgcgc cgccgagcag
     1981 cgtgatgaac tcacgccgtt tcaactgatc gaattgtatt cggtcctgcc agacaggggt
     2041 acaccggatt gcaggatacc gaattggcga agttcagtct ggaggtattg gcgcgatttt
     2101 gcaaccggcc gctgattggc aagcgacgat aaagagagcc cattgacccc attgccgatg
     2161 cccgggtagt gagctactca agttgttcgg tttgattagg gttcgaatcc tctgggcgcg
     2221 cttggcgaat gtgacgtcac ggccgccctt cggcgggtag cattcccgct gcgtatcccg
     2281 tgttcacatg ctcttttgag ggcggtctgc gatggctaag cgtgagcagg accccatcgt
     2341 ccgggctctg gtgcccgatc cgacggccgg gccgccgagc gttgcggtgc tccgcggctt
     2401 tctcggcgac tcgacgcaac ccgaccatta ccgcctttac ctgagcagga cgctcagcag
     2461 ttacgtcgat attcctgcgg accagattct gcacaccagc caattaccca acgacggcgg
     2521 cacccgggtg tgggtgccca gaaccctcga actgcgcttc gtgcggacgg tttctactga
     2581 ggttcaggcg cgattccttc agggatcgat cgtcaacagg catctggcgc tgggtggcgc
     2641 ctcgctggcc gcggaagccc ttcgcgcgca ggcacgacga ttgaggcgcg ttgacgaatg
     2701 gttcagcctc gagtgcggac actcggagga agatcacggc gcgacgtgtg gtgacacctg
     2761 ccccgcgtgt gaggaatagg cctgattgtt ggggcgcgtc aattgagtgc ccacgtgacg
     2821 ccgttaagcg cgattgacgt ccccgactat ctgttggccc gtggcctggt gagcacacgg
     2881 tccatcgtcg acagcgacct ggtcgtccac gagctgtcgg gccggaatcg caccttcgtc
     2941 gccgagtgca cgaacgcggc gagttacgtg ttgaaacaag gcgccacgcc agaagggaca
     3001 gccgccgctc acgaaggtga ggcgtaccgt gcgctttctg ctacccggtc catgggaccg
     3061 cacctgccgc agttccacgg gtacgacgcc gacgcccggg tcctcgtcgt cgagtacttg
     3121 cgcgacgccg aagacctggc caactatcac cgacaccggc gccgtccccc gatccggaca
     3181 gcttcggccg tgggcgcgat cctcgccgac ctgcatgagc tgcccgtcgc gcagaccggt
     3241 caggttgggt accgacggca gcgtccttcg gtgctgtctc tacaccgacc gggtccgcgt
     3301 atgctcgccg agtcgtcgcg ggtcactctc gaccttatcc gaatcatcca gggcacctat
     3361 ggattcgggt tccatcttga tgagctcaac gcagactggt cggctaccaa cctcatacat
     3421 caagacgtgc ggttcaagaa tttcgtgcgg acgacgtcgt cgaggggcat cgaccgtccg
     3481 cggctgaagt tgatcgattg ggagctggcc tgccttggcg atccgcgatg ggatattgga
     3541 tctgcgctcg gcgactatat gagcctttgg ttgacgtcca tcccggtgac tggatctgct
     3601 ccgaccgagc agtccgctga gctggcacgg tgtccgctag acggaatcca gcccgcgatc
     3661 cgcgcgtgct gggacgtcta cgtcaagcga cgccgtctcg gcgtatccgc cgacgcgttg
     3721 cttccggtcg tcgtccgctt cgccgcggca aggctggtgc agacagcgct cgagatcgcc
     3781 caggattcgg cgaacctgac caccgacatc gtcctgcacc tgcaggcggc gtacaacgtc
     3841 ttcgagcgac cgggcgtggc cgccgcgcac atgttcggcc tgacgtcgga caccatcgga
     3901 cgcgtcggtg gcgagaagcg atgaacgact attcggcgct gatggacgcg ggggtggctg
     3961 ccgttcgcat acactctgcc acctcctttt cgtggttcgg agagcgaaat cctcagttgt
     4021 ccaatacaat ccgggcagcg attagcccgt cggacgcgcg ggagtatctg ttgtattccc
     4081 tgcagctgca gctatatcgc aacttctatt gtccaggggc ccctgtcccg gcgggaatcc
     4141 taagtgtcgg aggacatcgg ggggtgtcgg tgcgggcgga tttcgtcgcc agactgtccg
     4201 ctgcgaacac gggcgtgggc tcgctcgaac cggggtggca cgttcacgcc gtcgatgcgg
     4261 atcgcgttgt ggtcgaacgg agtgggctgc ttgtctggat caagtatgag caactctgtc
     4321 tgactgatgg ggcgttgccc gagcccgggg tgtccgtgtc gatccggcag tccaaggagt
     4381 tgccgaggcg gtcacccggg ttctatctgg cgctcggcaa ccacggaatg aagtcgcgcg
     4441 acgtcgatcc ggtggtcagg ctgtactgga acgttatcgc cgaatccgcg ccgcaacttg
     4501 tggagttggt gaccacgcga ctcaacgctg ccgatattcc gtttcgtctc aaaacaatca
     4561 acgatgcccg tcgctacaca cgctgcgacg cagcggtgct ctatggtccg aagcggtgct
     4621 acccggcgat cgccgagatc gttgcgggga tccatgcgga gctggccgcg tcattggtgc
     4681 caggcacccc ggcgttcacc aaacgccttg cgccgggtct ggcgcttgcc gaagatcctg
     4741 gcaccggtga cagcttcggc atgcaccgct gtcggctttt cgctgaaggg atcctaaacg
     4801 cgcgcgagga cggccgccga tcgacctcgg cgcgacgcga ctcggtgatc gccgcgttcg
     4861 ctgctgcggg catcaccacc gaacggccct acctcaatcc cggctcggcc gacacctacc
     4921 aggcggtgtc cgaatgacgg tcgactacct cggcgtcgcg cggggtatcg gggcgcgctt
     4981 ggcggatact gcgatctggc acgaaggccg ctgcaattgg ctcggtgttc gcgtatttgg
     5041 caacggtcag gcaggtttcc gtgagacggt ggccgcgctc ggcggcgatc tctacagcgg
     5101 gtcgagcgga gtcgccctgt tcctcgccga actgcacgcc gtcgcgggag gtgacgcatt
     5161 cgccgggacc gcgatgggcg ccatcgaaca cgcactgggg cgcgctgaca gcgccgaaga
     5221 catcgggccg ggactctacg acggacggct aggaatcgcc ttggccgcgg cccggatcgg
     5281 tatgttggtc cgggacagtc aattgctcga gcgagcggcc gatgtggccg accgtgcaca
     5341 tcgcctagct tccgaactcg gcttcgacct gttgtccggg cgggccggag gcatcgtcgc
     5401 cctcctgatc ctggcgaagc tgctcgatga cgacggcctc atggaacacg ccacaaagtt
     5461 gggcgacgaa ctcatcgcaa cggtgacgga agacgggcgc taccaccccc ggtcagatgt
     5521 gcgtgcgaga cggcctctta ccggtctttc acacggcgcg gccggcgtcg gctacgccct
     5581 cggcgagcta ttccacaaca ccggagacgc ccggtacctg catgcagcgg aggctgcatt
     5641 cgcctacgag cgagcccatt tcgatccgga gacccgcaac tggccggact tccgacacga
     5701 caccggacgg cgccgcggtc aggttccccc ggttttcacc acccagtggt gccacggcgc
     5761 acccggtatc ggactgaccc ggttacgcgc gtatcgcctc cgcgatgacg actcatgcct
     5821 gaccgaggct gtcgccgcga tcgcgaccac ccgcgacgcg gttgccgtga gtctggccaa
     5881 gcctggcgcg aattactcac tctgtcacgg tctttccgga aatgccgaag tgcttttgta
     5941 cgccgaggag agcctgggct ccgaaggcgc ggagcatcga gccctcgcga ttcgcgtcgc
     6001 ggaacatggc atcgagcgct ttgcagcgcc gaaccgcatg tggccgtgcg gaactcgcga
     6061 aggtgtgaca cccaacctga tgctcggtct ggcgggaatc ggccacttct acctcaggtt
     6121 gcacgactcg actgtgccgt cggttctcct gatgcggccg gaatctttcg tcggtcgtgc
     6181 ggtcatcgac gggcgggtgg gtgaggaccc gcagccagga tggcagcgcg gcgatcctga
     6241 gcagtcggcg agcgtgaccc tccggtagtg aagcgagcgg tccaccgtcg agccgccgtc
     6301 cggccctcaa agcggaaggc tgatgccgca cgttccccag gtagctcaac accattgtcg
     6361 tggcctgcgg tgcttggttc ctacggccgg ggagcctgaa caacgctgtc ggcgtgcgcg
     6421 agcacgaaag acccgcgtac cggggcagcg aatcgcctgc ggctgcacga attaatgccc
     6481 agtgccggcg tcggggcgtt ggcccccagg ccgccggggc gcagcatggg tttatgtgtg
     6541 atggtggcgg ctcgcccacc gcggttgcgg cgaccctccc gcggccggcg gcgggaacgg
     6601 cggtggaccc catcgctttg gaaccggtcg acgaggtggt catcacgacg ctcgtggaca
     6661 acagctacga cacgctgatg ggcgacaccg gtccggcccg ccgcgcgccg ttgcccgggg
     6721 tcgctgcttt agagattggc attggtgccg accaataccg gctggcacac caggatcgcg
     6781 aataaccgcc actcgtcttg tgctgggtgc gatctccttg agcaattcaa ggcgttttcc
     6841 gctcatgctg aattcgacac tcatgaaacc ggtggcgttg ccgcccggcc gcgccaagct
     6901 ctcgacgaag ccggcggcga ccggatcgga ggcgaccggg aacacgatcg gcaccgtgcg
     6961 ggtcgccggc agcaacggcc ccagggtcgg ggcgccatgg gc
//
LOCUS       DASRJA010000638         4983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_38621,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000638 DASRJA010000000
VERSION     DASRJA010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38621"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFC01_28555"
     CDS             complement(<1..440)
                     /locus_tag="VFC01_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83604.1"
                     /translation="MRKVFLGSEAVGQGALTAHELRRWNQRIFRDVYVPKQHKLSLSD
                     RIEGAWLRSGRQGVVAGLAASALHGAQWIDDDVPIELIWDNTRSPSGLVVRNETLDDD
                     EITRVAGVPVTTRLRTAFDLGRLLDRDQAVARLDALMWAQIFSV"
     gene            complement(539..937)
                     /locus_tag="VFC01_28560"
     CDS             complement(539..937)
                     /locus_tag="VFC01_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83605.1"
                     /translation="MADDEFRVLVELGSDDDPLSLWEKLRSLDLDDDAQERLGSRVTI
                     TRDGDRMILYTATLSDAEEAERTVQALVAEDDLSAEYSIARWNGDEQEWEDPADGDPV
                     PDDAPEVAVPDPSYVILDAYKPEFLRDLGL"
     gene            complement(948..1559)
                     /gene="recR"
                     /locus_tag="VFC01_28565"
     CDS             complement(948..1559)
                     /gene="recR"
                     /locus_tag="VFC01_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZN83606.1"
                     /translation="MFEGPVQDLIDELGKLPGIGPKSAQRIAFHLLSVEPPDIDRLTA
                     VLNKVRDGVKFCAVCGNVSDEDRCRICSDARRDPSVVCVVEEPKDVQAVERTREFRGR
                     YHVLGGALDPLSGVGPEQLRIRELLNRIGERVDGVDVTEVIIATDPNTEGEATATYLV
                     RMLRDIPGLTVTRIASGLPMGGDLEFADELTLGRALAGRRAMA"
     gene            complement(1561..1893)
                     /locus_tag="VFC01_28570"
     CDS             complement(1561..1893)
                     /locus_tag="VFC01_28570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZN83607.1"
                     /translation="MQPGGTPDMSALLAQAQQVQQQLMEAQEALAAAEVHGQAGGGLV
                     QVTMKGSGEVVAVSIDPKIVDPSDVETLQDLIVGAITDASKQVTILAHDRLGPLAGGM
                     GGLGLPEL"
     gene            1993..2748
                     /locus_tag="VFC01_28575"
     CDS             1993..2748
                     /locus_tag="VFC01_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3717 family N-acetylmuramoyl-L-alanine
                     amidase"
                     /protein_id="HZN83608.1"
                     /translation="MPASLRVAAAIAAGLLVAASTLVSPASAAPANIAGMIVFLDPGH
                     NGSNDDSIGRQVPTGRGGTKNCQESGTSTDDGYPEHTFTWDTTLRIRQALTALGVRTA
                     MSRGNDTGLGPCVDERAAMANALQPNAVVSIHGDGGPPTGRGFHVLYSSPPLNQTQAG
                     PSVQFAQTMRDQLAASGFVPSTYTGSNGLDPRSDIAGLNLAQYPSILVECGNMKNPVD
                     SGLMKTPEGRQKYADAVVRGIAGFLGTQPPRTN"
     gene            complement(2777..3217)
                     /locus_tag="VFC01_28580"
     CDS             complement(2777..3217)
                     /locus_tag="VFC01_28580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN83609.1"
                     /translation="MGQVSASSTVLIDASPEVVLAAVADYATVRPKILSSQYSDYKVL
                     EGGQGAGTVASWKLQATKSRVRDVKANVDVAGHTVIEKDANSSMITNWTVAPAGPGSS
                     VTVKTSWQGAGGIGGFFEKTFAPLGLRKIQGEVLENLKKAVEDR"
     gene            3281..4660
                     /locus_tag="VFC01_28585"
     CDS             3281..4660
                     /locus_tag="VFC01_28585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN83610.1"
                     /translation="MSVEGSDARAAHAAGVQQLLASYRAIPPNATVRLAKPTSNLFRA
                     RAKTTSKGLDVSGLTGVIAVDPANRTADVAGMCTYEDLVAATLPYGLAPLVVPQLKTI
                     TPGGAVTGLGIESTSFRNGLPHESVLEMDILTGTGDVVTASRDLCSDLFHAFPNSYGT
                     LGYSVRLRIKLESVKPFVALRHLRFHSLDDLVAAMDRIIETGGYDGTPVDYLDGVVFS
                     ATEGYLTVGIQTSTPGPVSDYTGQQIYYRSIQHAGGEKHDRLTIHDYLWRWDTDWFWC
                     SRAFGAQNPRLRRLWPRRYRRSSFYWKLIAYDQRFNIADRIEKRNGRPPRERVVQDIE
                     VPIERTAEFLDWFLANVPIEPIWLCPLRLRGDDRWPLYPIRPHHSYVNVGFWSSVPVG
                     PTEGHTNKLIERKVSTLDGHKSLYSDAYYSHDEFDDLYGGETYKTVKKTYDPDSRLLD
                     LYAKAVQRR"
     gene            4657..>4983
                     /locus_tag="VFC01_28590"
     CDS             4657..>4983
                     /locus_tag="VFC01_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZN83611.1"
                     /translation="MTTFKDQTHAPVRRLTLAEILEIFAAGQLPLKFTAYDGSMAGPE
                     DAELGLDLLTPRGTTYLATAPGDLGLARAYVSGDLEPHGVHPGDPYELLKALADKMDF
                     KRPPARV"
BASE COUNT          865 a         1762 c         1500 g          856 t
ORIGIN      
        1 tcgacggaga agatctgcgc ccacatcagt gcgtcgaggc gcgccaccgc ttgatcgcga
       61 tctaggagtc ggcccaggtc gaacgccgtc ctcagacggg ttgtcacggg aacgcccgcg
      121 acccgggtga tctcgtcgtc gtccaacgtc tcgttgcgga ccaccaatcc gcttggcgag
      181 cgggtgttat cccagatcag ctcgatggga acgtcatcgt cgatccattg ggcgccatgc
      241 aacgcggatg cggccaagcc cgcgacgact ccttggcggc cggatcgcaa ccaggcaccc
      301 tcgatacggt cactgagtga tagcttgtgt tgctttggga cgtacacgtc gcggaagatc
      361 cgctgattcc agcgccgcaa ctcgtgcgcg gtgagcgccc cctgccccac ggcctcgctg
      421 ccgagaaaga ccttcctcat gccgagatgc tggcaccacg ggccgacacc tccgcgagac
      481 tgcacttatt gcgtggaatt acgagaaggg ccccgcaaca actccagttt cgcggcgtct
      541 acagacctaa gtcgcgcaga aactccggct tgtacgcatc gagaatgacg tagctcgggt
      601 cgggaaccgc aacctcggga gcatcgtcgg gcaccggatc gccgtcggcc gggtcctccc
      661 attcctgctc gtcgccgttc caccgagcga tcgagtattc ggcgctcagg tcatcctcgg
      721 cgacgagcgc ctggacggtg cgttccgcct cctcggcgtc ggacagcgtc gctgtgtaga
      781 gaatcatccg gtcgccgtca cgcgtgatcg tcacccgcga gccgagtcgc tcctgagcgt
      841 cgtcgtccag atccagcgac cgtagcttct cccacaggga aagtggatcg tcatcgctgc
      901 ccagctcaac caggaccctg aactcgtcgt ccgccatgag cgcaaaccta agccattgcg
      961 cggcggccgg ccagggcccg gccaagcgtc agttcgtcgg cgaattccag gtcgccgccc
     1021 atcggcaggc ccgacgcgat tctcgtcacg gtgaggccgg ggatgtcgcg cagcatccgg
     1081 accagatacg tcgcggtcgc ctcgccctca gtgttgggat ccgtcgcgat gatcacctcg
     1141 gtgacgtcca cgccgtcgac gcgttcgcca atccgattca acagctcacg gatccgaagt
     1201 tgttctggcc caacgccgga cagcggatcc aatgccccac ccaggacgtg gtagcggccg
     1261 cggaactcgc gggtgcgctc gacggcctgc acatccttcg gctcctcgac cacacacacc
     1321 acagaggggt cccgacgcgc atctgaacag attcggcagc ggtcctcgtc ggaaacgttg
     1381 ccgcacaccg cgcagaactt cacgccgtcg cgaaccttgt tcaacactgc ggtcagacgg
     1441 tcgatgtccg gcggctcgac gctcaacaag tgaaaagcga tccgctgtgc gctcttcggc
     1501 ccgataccgg gcagcttgcc gagctcgtcg atcagatcct ggacggggcc ttcgaacaag
     1561 tcacagctcc ggcagaccca ggccacccat gccgccggcg agcggcccga gccgatcgtg
     1621 cgccaggatg gtgacctgct tggaggcgtc ggtgatcgcg ccgacgatca gatcctgcag
     1681 cgtctcgaca tcggaggggt cgacgatctt cgggtcgatc gacactgcca ccacttcacc
     1741 gctgcccttc atggtgacct gcaccagacc gccgccggcc tgcccgtgca cctcggcagc
     1801 ggccagcgct tcctgcgcct ccatcaactg ctgctgcact tgctgagcct gagcgaggag
     1861 tgccgacatg tcgggcgtgc ctccgggttg catgacagtc cccttgcgtc ttggtctcga
     1921 cttggttgcg ctcatccaca agcggcggtt tggtctttca gcctagtcgc caggcacgct
     1981 acggtggcgg ccgtgcctgc cagcctgcgt gtcgccgccg cgatagccgc cggtctgctc
     2041 gtcgccgcat cgacgctcgt cagcccggcc tcggcggccc cggcgaatat cgccggcatg
     2101 atcgtgttcc tcgaccccgg ccacaacggc tccaacgacg actccatcgg cagacaggtg
     2161 cccaccggcc gcggcggcac caagaattgc caggagagtg ggacctcgac cgacgacggc
     2221 tatcccgaac acaccttcac ctgggacacc acgctgcgca tccgccaggc gctgaccgcc
     2281 ctcggcgtgc gcaccgcgat gtcgcgcggc aacgacaccg gcctcggccc gtgcgtcgac
     2341 gaacgcgccg cgatggcaaa cgcgctgcaa cccaacgccg tcgtcagcat ccacggcgac
     2401 gggggcccgc ccaccggccg cggcttccac gtcctctact cgtctccgcc gctgaaccag
     2461 acacaggcag ggccgtccgt ccagttcgcc cagaccatgc gtgatcagct cgccgcgtca
     2521 ggatttgtgc cgtccaccta caccggctcg aacggcctcg acccgcggtc ggacatcgcc
     2581 ggcctgaacc tggctcagta cccgtcgatc ctcgttgagt gcggcaacat gaagaatccc
     2641 gtcgactccg ggttgatgaa gacccccgag ggccgtcaga agtacgccga cgccgtcgtc
     2701 cgcggtatcg cgggcttcct tggcacccag ccgccgcgga ccaactagcc ctgctcgctg
     2761 cccaatgtcg gaccgcttac cggtcctcca cggccttctt gagattttcc agcacctcgc
     2821 cctggatctt gcgcaacccc agcggcgcga acgtcttctc gaagaagccg ccgatgccgc
     2881 ccgcgccctg ccacgacgtc ttgaccgtga ccgacgaacc cggccccgcc ggagcgacgg
     2941 tccagttggt gatcatcgac gaattggcgt ctttctcgat cacggtgtgg cccgccacgt
     3001 cgacgttggc cttgacgtca cgcacgcgcg acttcgtcgc ttgcagtttc cagctggcga
     3061 cggtgcctgc gccctgcccg ccctcgagca ccttgtaatc gctgtactgc gaggacagaa
     3121 tcttcggccg cacggtcgca taatccgcca ccgccgccaa caccacttcc ggtgaggcgt
     3181 caatcaagac ggtgctggac gcgctgacct gtcccatgag atagctctcc tgtcgtgttg
     3241 tgcggtcgca tcggctgcgg ccgcggacta gcgtatatcc gtgtcagttg aggggagcga
     3301 cgcacgggct gctcacgctg cgggcgtgca gcagcttctc gcgagctacc gggccattcc
     3361 tccgaacgcc accgtccggc tggccaaacc tacgtcgaac ctgttccggg cgcgggccaa
     3421 gacgacgagc aaggggcttg acgtctcggg gctgacgggc gtcatcgccg tcgatcccgc
     3481 caaccgcacc gccgacgtcg ccggcatgtg cacctatgag gacctcgtcg cggccaccct
     3541 cccgtacggc cttgccccgc tggtggtgcc gcagctgaag acgatcacgc cgggtggggc
     3601 ggtcactggg ctgggcatcg agtcgacttc gttccgtaac ggcctgccgc atgagtccgt
     3661 gctggagatg gacatcctca ccggtaccgg cgacgtggtc accgcaagcc gcgacctctg
     3721 ctcggatcta ttccatgcgt tcccgaattc ctatgggaca ctgggatatt cggtccgatt
     3781 gaggatcaag ctggagtctg taaagccgtt tgtggcgctg cggcacttga ggtttcactc
     3841 gttggacgac ctcgtcgccg cgatggaccg catcatcgag accggcgggt acgacggcac
     3901 accggttgat tacctcgacg gggtggtgtt cagcgccaca gagggctact tgacggtcgg
     3961 aattcaaaca agcacacccg gccccgtcag cgactacacc ggccagcaga tctactaccg
     4021 ctccatccag cacgccggcg gtgagaagca cgaccggctc accattcacg actacctgtg
     4081 gcggtgggac accgactggt tctggtgctc gcgggcgttc ggcgcacaga atccccggct
     4141 gcgccggctg tggccgcggc gctaccggcg cagcagcttc tactggaagc tgatcgccta
     4201 cgaccagcgg ttcaacatcg ccgaccgcat cgagaagcgc aacggccgcc cgccccggga
     4261 gcgcgtcgtg caggacatag aagtaccgat cgagcgcacc gccgagttcc ttgactggtt
     4321 cctggccaac gtccccatcg aaccgatctg gctgtgtccg ttgcgactcc gaggtgacga
     4381 tcgctggccg ctgtacccga tccgcccgca ccactcctac gtcaacgtcg gcttctggtc
     4441 ctcggtaccc gtcggaccga ctgaaggcca tacaaacaag ctgatcgaac gcaaggtcag
     4501 cacgctcgac gggcacaaat cgctgtactc cgacgcttac tactcccacg atgagtttga
     4561 cgacctctac ggcggggaga cttataagac cgtaaagaag acctacgacc ccgactcacg
     4621 gctgctagac ctatatgcga aggcggtgca acgacgatga cgacattcaa agatcagacc
     4681 catgcgccgg tcaggagact cacgctggcc gagatcctcg agatcttcgc ggccgggcaa
     4741 cttccgctga agttcaccgc gtacgacggc agcatggccg gacccgagga cgctgagctc
     4801 ggccttgatc tgttgacgcc gcggggcacc acctacttgg ccaccgcgcc cggcgatctc
     4861 ggcctggccc gcgcctacgt gtcgggcgac ctggagccgc acggcgtgca tcccggcgat
     4921 ccctacgagc tgctcaaggc gctcgccgac aagatggact tcaagcgtcc gcccgcgcgg
     4981 gtg
//
LOCUS       DASRJA010000639         5060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_643264,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000639 DASRJA010000000
VERSION     DASRJA010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643264"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1131)
                     /locus_tag="VFC01_28595"
     CDS             complement(112..1131)
                     /locus_tag="VFC01_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN83612.1"
                     /translation="MIAAMLDHRTDLDLVGAKVYSDAKHGKDVGVLVGRDPVGVLATA
                     DTAEAIATAADCVLYTPRTANLDEVCAILASGKNVATTAFLFHPRRLPQRDRDRLLAS
                     CEQGRASVHGSGLNPGNLSGVLPLALSGMSRRIDKITLQERADWSVYESTGITFDNMA
                     FGQPVEQISPTTNEFLAFNSSIFTEQVWLLADALNACIDEVTATVEAVAAREDHQIFD
                     HVLRTGTTAGQRWNWVGRRNGEPLIEIETLWTVGGEYPGHWPRPKDGWTLTIEGDPSM
                     RTHFFALASFTRPANMEEHVRSANVATAMQVLNAVPAVCDAAPGFATTATLPLVRSRT
                     GFGNG"
     gene            1477..1875
                     /locus_tag="VFC01_28600"
     CDS             1477..1875
                     /locus_tag="VFC01_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZN83613.1"
                     /translation="MKFSGITARRRIAGVAAGCVLGGVAAATIAAPSATAAPDCSAAG
                     VSNTVSTTIGSARAYLNAHPGANQVVGAAFSQPRDAAAGTLRGYFTANPQEYYDLRGI
                     LAPIGDTQRQCNVSVLPPELASAYSEFMAG"
     gene            1960..2391
                     /locus_tag="VFC01_28605"
     CDS             1960..2391
                     /locus_tag="VFC01_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83614.1"
                     /translation="MRSTRTAAFAASVVLVGCVAPTATAQADPAPAPPPAPKTTIDAD
                     GTYAVGKDIQPGRYSSAGPIDDGACYWKRVNGTDIIDNALSKKAQVVEIAPTDTTFTT
                     NECQPWQLTDAPLPAQAGPGDVLGQLGQILLKPAPPPPSNP"
     gene            complement(2395..2826)
                     /locus_tag="VFC01_28610"
     CDS             complement(2395..2826)
                     /locus_tag="VFC01_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZN83615.1"
                     /translation="MTATTFDPHTLLTESRLGVLATIKSNGLPQLSPVTPYYDRETGV
                     IYVSMTEGRAKTANLRRDPRAALEVTSSDGWAWATAEGAVTLTGPGSEPHGPEVEALV
                     DYYRSAAGEHPDWDEYRAVMVSDRRVLMALTVDRVYGEKIR"
     gene            2882..3367
                     /locus_tag="VFC01_28615"
     CDS             2882..3367
                     /locus_tag="VFC01_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HZN83616.1"
                     /translation="MADLHPGIAALAPLLGTWSGQGAGEYPTIESFSYLEEVTFGHVG
                     KPFLVYGQRTKATDDGRPLHAETGFLRVPSPDRVELVLAHPTGITEIQEGTLSVDGTT
                     LELELTATTIGRTSSAKDVTALSRSIRIDGDELTYTLRMGAVGQPLQHHLAATLHRTH
                     P"
     gene            3418..4659
                     /gene="lipE"
                     /locus_tag="VFC01_28620"
     CDS             3418..4659
                     /gene="lipE"
                     /locus_tag="VFC01_28620"
                     /EC_number="3.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase LipE"
                     /protein_id="HZN83617.1"
                     /translation="MTSDGRIRVPADLDAVTAVGDEDHSAVDSAAVERIWQAVRHWYS
                     AGMHPAIQLCLRHNGRVVLNRAIGHGWGNGPDDPPDAEKVAVTTETPFCVYSSAKAIT
                     TTVVHMLAERGVLSLDDRVCDYLPSYTSHGKDRTTIRHVMTHSAGVPFATGPRPDLRR
                     MDDSEYAREMLGNLKPIYRPGLVHIYHALTWGPLVREIVSAATGKNIRDVLATEILDP
                     LGFRWTNYGVAKADVALVAPSHPTGKPLPAPIAAAFRTAVGGTMHQIIPYSNTPQFLT
                     GVVPSSNTVSTADELSRFAEILRRGGELDGVRILSPETLRGATRECRRLRPDLATGLA
                     PLRWGTGYMLGSNRFGPFGRNAPAAFGHTGLVNIAIWADPERGLSAGLVSSGKPGRHR
                     EANRYPAVLDRIAAEIPRVQA"
     gene            4727..>5060
                     /locus_tag="VFC01_28625"
     CDS             4727..>5060
                     /locus_tag="VFC01_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN83618.1"
                     /translation="MASQSAASQCTIERRRVGEIEVLSVAGVVDMLTASQLEEAIAAA
                     AKDSPQGVIVDLGAVDFLASAGMGILVAARHELPAAVQLAVVADGPATSRPLKLVGIA
                     DVVGLHATL"
BASE COUNT          856 a         1685 c         1680 g          839 t
ORIGIN      
        1 cgccgagaag gccttcgtag aacgtcgcca gacccttcgg gtccttgcag tcgaccacca
       61 ccgagcgaag ttgtccgatc atgatcgctg ggttaccaat cgcgctgcgc cctaaccgtt
      121 gccgaatccg gtgcggctgc gcaccagcgg cagcgtcgcc gtcgtggcga agccgggcgc
      181 cgcgtcgcac actgcgggta ccgcgttgag aacttgcatt gcggtcgcta cattcgccga
      241 gcgcacgtgt tcttccatat ttgccggacg ggtgaagctc gccagcgcga agaagtgggt
      301 gcgcatggac gggtcgccct cgatggtcaa cgtccagccg tccttcggtc gcggccagtg
      361 cccgggatac tcgccgccca cggtccacag cgtctcgatc tcgatcagcg gttcgccatt
      421 gcggcggccg acccagttcc agcgctggcc agccgtcgta cccgtccgca gcacgtggtc
      481 gaagatctgg tggtcttcgc gggcggcgac ggcctcgacg gtggcggtga cttcgtcgat
      541 gcaggcgttc agcgcatcgg cgagcagcca cacctgctcg gtgaagatcg agctgttgaa
      601 cgccaggaac tcgttggtcg tcgggctgat ctgctcgacg ggctgaccga atgccatgtt
      661 gtcgaacgtg atgccggtgc tctcatacac cgaccagtcg gcacgctcct gcagcgtgat
      721 cttgtcgata cggcggctca tccctgacaa cgccagcggc agcacaccgg acaggttgcc
      781 cgggttcagg ccgctgccgt gcacgctggc tcggccctgc tcacaggagg ccagcaggcg
      841 gtcacggtca cgctggggca ggcggcgtgg gtggaacagg aaggctgtcg tggcgacgtt
      901 cttgccgctg gcgaggatcg cgcagacctc gtccaggttg gctgtgcgcg gggtgtagag
      961 cacgcagtcg gctgccgtcg cgatggcctc tgcggtgtcg gccgtagcga gcacgcccac
     1021 ggggtcgcgt ccgacgagca cgccgacgtc cttgccgtgc ttggcgtcgg agtacacctt
     1081 ggccccgacg aggtcgaggt cggtccggtg gtcgagcatc gcggcgatca tctcggcgcc
     1141 gaccgcgccg gtgccccact gaatgactcg atatggcggc gtcacagcgg gcaggctacc
     1201 gcccattgaa taacaattcg atcactatgc aaatcacgat caattaagag ctcccggcaa
     1261 attttgagag ttttcaagat ctccgtcccg atctcgttga tggcttagcg aaatcggccc
     1321 aatttgagtg cgatatcacc ccattgggct gtaacgcgtg tctgtgccgc cgatgcgcgc
     1381 ctgcgcggtg aatttgcacg ctttctaatc cgctacgttg gcattgggga cggggaaatc
     1441 tgcgacagaa tcgcaattca aaggaagaaa acgatcatga aattcagtgg tatcactgcg
     1501 cgccggcgca tcgccggtgt cgctgccggc tgcgtcctcg ggggcgtcgc tgcagcaacc
     1561 atcgccgcgc caagcgcgac ggcagcgccc gactgttcgg ccgccggggt gtccaacacc
     1621 gtcagtacga cgattggctc cgcgcgcgcc tacctcaacg ctcacccggg tgccaaccag
     1681 gtcgtcggag cggcgttcag ccagccgcgt gacgcggcgg ccggcactct gcgcggctac
     1741 ttcaccgcca acccgcagga gtactacgac ctgcggggca tcctggcgcc gatcggggac
     1801 acccagcgcc agtgcaacgt gtcggtgttg ccgccggagc tggcgtcggc ctactccgag
     1861 ttcatggccg gctagtttca cccacgaaaa cccgccgctg cagccctcgc tgtggcggcg
     1921 ggtttttcgt tagcaggtct tagcgactac tctggggcca tgaggtcgac cagaacagcc
     1981 gcgttcgcgg cgtccgtcgt tctcgtcggc tgcgtcgcgc ccaccgcgac cgcccaggcc
     2041 gatcccgctc cggcaccgcc gcccgcgccc aagacgacca tcgacgccga cggcacctac
     2101 gcggtcggca aggacatcca gccaggcagg tacagctcgg ccggcccgat cgacgacggc
     2161 gcgtgctact ggaagcgcgt caacgggacc gatatcatcg acaatgcgtt gtccaagaag
     2221 gcgcaggtcg tggagatcgc accgaccgat acgacgttca cgacgaacga gtgccagcca
     2281 tggcaactga ccgatgctcc gctgcccgcc caagcggggc ccggcgacgt gctcggccag
     2341 ctggggcaga tccttctcaa gcctgcgcca cccccaccga gcaatccgta acggtcagcg
     2401 gatcttttcg ccgtacacgc gatccaccgt caacgccatc aagacccggc gatcggacac
     2461 catcacggcg cgatactcgt cccagtccgg gtgctccccc gcggcgctgc ggtagtagtc
     2521 gacgagcgct tcgacctccg gaccgtgcgg ttcggaaccc ggcccggtaa gggtcacggc
     2581 gccttcagcc gtcgcccacg cccacccgtc ggaactggtg acctcgagcg cagcgcgggg
     2641 atcacggcgc aggttcgccg tcttggcacg gccctcggtc atcgagacgt agatgacacc
     2701 ggtttcgcgg tcgtagtagg gcgtcacggg ggaaagctgc ggcaggccgt tggacttgat
     2761 ggtggcgagc acaccgagcc ggctttcggt gagcagcgtg tgcgggtcga acgtcgtggc
     2821 tgtcatgtca acgggcaacc cacgaccgca tgcgcgctat tccgtccgta ccctgacgaa
     2881 cgtggccgat ctgcatcccg gcatcgctgc cctggctccc ctgctgggca cgtggtcggg
     2941 gcagggcgca ggcgaatacc cgacgatcga gtcgttcagt tatctcgagg aagtgacgtt
     3001 cggtcacgtc ggcaagccgt tcctggtcta cggccagcgg acgaaggcca ccgatgacgg
     3061 tcgcccgttg catgcggaga caggattcct tcgggtgccg tcgccggatc gtgtcgaact
     3121 cgtgctggcc catccgaccg gcatcaccga gatccaggag ggcacgctgt cggtcgacgg
     3181 aacgacgctg gagctggagt tgaccgcgac gacgatcggt cgcacatcgt cggccaaaga
     3241 cgttacggca ctgagccgtt cgatccggat cgacggtgac gaactgacct acacgctgcg
     3301 aatgggcgcc gtcggtcagc cgctgcagca ccatctggcg gcgacgctgc acaggacgca
     3361 cccgtgagcg cccgaagacg actgcgagcg agtaccggag cgagcgggag tcgaggcatg
     3421 acctctgacg gcaggatccg ggtgcccgcc gacctcgacg cggtcacggc ggtgggcgac
     3481 gaagaccatt ccgcagtcga ttccgcggcg gtcgagcgca tttggcaggc ggtgcggcac
     3541 tggtattcgg ccggcatgca tcccgcgatc cagctgtgcc tgcggcacaa cggcagggtc
     3601 gtgctcaacc gggcgatcgg ccacggctgg ggtaacgggc ccgacgatcc gcccgacgcc
     3661 gaaaaggtcg ctgtcacaac ggaaaccccg ttctgcgtgt actcctcggc caaggcgatc
     3721 accacgaccg tcgtgcacat gctggccgaa cgtggcgtgt tgtcgctcga cgaccgggtc
     3781 tgcgactacc tgcccagcta caccagccac ggcaaggacc gcaccacgat ccggcacgtg
     3841 atgacgcaca gcgccggggt gccgtttgcc accgggccac ggcccgacct gcggcgcatg
     3901 gacgacagcg agtacgcccg cgagatgctc ggcaatctga agccgatcta tcggccggga
     3961 ctggtgcaca tctaccacgc actgacgtgg ggtccgctcg tgcgcgagat cgtttcggcc
     4021 gccaccggca agaacattcg cgacgtcctt gccaccgaaa tcctcgaccc cttgggcttc
     4081 cggtggacga actacggcgt cgcgaaggcc gacgttgccc tggtggcgcc gagccatccg
     4141 acgggcaagc cgctgccggc acccatcgca gcggcattcc gcaccgcggt gggcggcaca
     4201 atgcaccaga tcatcccgta ctcgaacacg ccgcagttcc tcacgggtgt ggtcccgtcg
     4261 tccaacacgg tctcgaccgc cgacgagcta tcccggttcg ccgagatcct gcgccgtgga
     4321 ggagaactgg acggcgtgcg catcctgtca ccagagaccc tgcgcggggc gacgagggag
     4381 tgcaggaggc tgcggccgga tctcgcgacc gggctggcac cgctgcgctg gggcaccggc
     4441 tacatgctgg ggtccaaccg gttcgggccg ttcggccgca acgcgcctgc ggcgttcgga
     4501 cacaccggtt tggtcaacat cgcgatctgg gccgatccgg aacgggggct gtcggcgggg
     4561 ctggtcagca gcggcaaacc gggcaggcat cgcgaagcca accgctaccc ggccgtgctg
     4621 gaccgcatcg cagcagagat accgcgcgtc caggcatgat gtccgatgtt tggcaaccgg
     4681 tagttttggg aaccgggtgg tagccaattt ttggtcggag gtaatcgtgg cgagccaatc
     4741 agcagccagt cagtgcacaa tcgagcgccg gcgggtcggc gagatcgagg tgctgtccgt
     4801 ggcgggcgtg gttgacatgc tcacggcgtc gcagctcgag gaggcgatcg ctgcggcggc
     4861 taaggactca ccgcaaggcg tgatcgtcga tctgggcgcg gtcgactttc ttgcgtcggc
     4921 cggcatgggc atcctggtcg ccgcgcgcca tgagctgccc gcagccgtgc aactggccgt
     4981 cgtcgctgac ggtcccgcaa ccagccggcc actcaagctc gtcggaatcg ccgacgtcgt
     5041 cgggctgcac gccacattgg
//
LOCUS       DASRJA010000640        12674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3549179,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000640 DASRJA010000000
VERSION     DASRJA010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12674
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3549179"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VFC01_28630"
     CDS             complement(<1..177)
                     /locus_tag="VFC01_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83619.1"
                     /translation="MQVRTGTAKSGDLDIYYEDMGDPNDPAVLLVMGLGAQLLLWRTE
                     FCEKLVDQGLRVIRY"
     gene            complement(178..1524)
                     /locus_tag="VFC01_28635"
     CDS             complement(178..1524)
                     /locus_tag="VFC01_28635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HZN83620.1"
                     /translation="MSTTKHREVAKLDRVPLPVEAARIGATGWQITRTGARVVSTLAG
                     KGSLQQKIIKQIPKAFSDLGPTYVKFGQIIASSPGAFGEHMSREFRSLLDAVPPADPD
                     EVHKLLTEELGDEPKSLFKTFEDTPFASASIAQVHYATLHSGEEVVVKIQRPGIRRRV
                     AADLQILKRGAQIVELAKLGRRLSAQDVVADFADNLAEELDFRLEAQSMDAWVSHMHA
                     SPLGRNIRVPQVYWDLTSERVLTMERVGGIRIDDAAAIRKAGFDGTELVKALLFSVFE
                     GGLRHGLFHGDLHAGNLYVDADGKIVFFDFGIMGRIDPRTRWLLRELVYALLVKKDHA
                     TAGKIVVMMGAVGTVKPEAQAAKDLEKFATPLTMSTLGDMSYADIGRQLSTLADAYDV
                     KLPRELVLIGKQFLYVERYMKLLAPKWQMMSDPQLTGYFANFMVEVSREHTEEVEA"
     gene            1710..3011
                     /locus_tag="VFC01_28640"
     CDS             1710..3011
                     /locus_tag="VFC01_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN83621.1"
                     /translation="MATLSIEPQRTSMRRVAAASFVGSAIEYYDFYIYGTAAALVFPK
                     VFFPHLGTTVGTVASMATFAAAFLSRPLGAAFFGHFGDRLGRKSTLIATLLIMGLSTL
                     AVGLVPGAVSIGMAAPLILLTLRLVQGFAVGGEWAGAALLSAEYAPAQARGRYGMFTQ
                     MGVGSGLVMSSLLFLVVNQTIGESSRAFIDWGWRIPFLFSAVLIVIALYVRLNVAETP
                     VFAEQKARDTARVMPLAALFRSQRREVVLAAGSMIGFFTLGYMANAYLMSYAHTHVGF
                     SPKLILSVGLLGGVVVVVFNVLGAVLSDAIGRRRVIMTAFAIGVPWSFVVLPLVDTGN
                     AVLFALALAGTYAVAGTAYGPMAAFIPEIFGTRYRYSGAGLSLNLAGLVGGAVPTIVA
                     APLQATWGVAAVGAMMAVVVVASLVCTAALPETKGAALQRS"
     gene            3150..3422
                     /locus_tag="VFC01_28645"
     CDS             3150..3422
                     /locus_tag="VFC01_28645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83622.1"
                     /translation="MNTNNKSRIFTRFVAGPIAAAGILGGVAIGLAAGANADPGPSDH
                     PSGGYSSEFRSGHDSRDSYRDFRPDFWDFWFWHHHRDHSFGWHRDS"
     gene            complement(3493..4389)
                     /locus_tag="VFC01_28650"
     CDS             complement(3493..4389)
                     /locus_tag="VFC01_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN83623.1"
                     /translation="MQTRSGTAACGDVELFYEDLGDPADPPVLLIMGVGAQLPMWPDG
                     FCAQLVKCGYRVIRFDHRDTGLSTKMHGTRAKGSVYPRVLRYVVGRSSPVPYTLVDLA
                     EDVKGLLDHLGIERAHVVGASMGGMIAQVLAGAHAARVRSLGLIMTSAGTPMSSIPKW
                     RVIRLAFNAPPKNAPVEDKLATEVRNISIINGPNFLPPVDQLRRRVEELTARSDYKQG
                     MLRQFDAILGTGSLMRYTRRITAPTVVVHGSKDPMVRPRNGRNVARAIDGARYVVVDG
                     MGHDLPEPVWRPVVEALTENMA"
     gene            complement(4477..5076)
                     /locus_tag="VFC01_28655"
     CDS             complement(4477..5076)
                     /locus_tag="VFC01_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZN83624.1"
                     /translation="MPGTPPPASGERQSLIEFLRFNQDAFFSAAYGLTDEQARSAPAA
                     SALSIGGLVKHAAGVQKGWVERAAAAPDFPPKDDRPMEEIMASYADEHTMRDDETLDE
                     LLDRLEKQNEETLRVFAEADLDTPVPVPHEVPWFPQDVENWSVRWVALHLIEELTRHA
                     GHADIIRETIDGATMYELMAANEEWPETDFLKRWKPAQV"
     gene            complement(5080..5622)
                     /locus_tag="VFC01_28660"
     CDS             complement(5080..5622)
                     /locus_tag="VFC01_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZN83625.1"
                     /translation="MSTDTKDLTFRLAEQLDWHWTNQLRPRLEGLTDEEYFWQPVPDC
                     WTVHPDGSIDFTYPEPQPVPFTTIAWRLAPVIVGVFEMRNHSHFGGPPADYQSWNYAT
                     DAKTALQQLDAAYAKWIAGVRGLSEDDLEQPCGPSEGPYADYPFIALVLHINREAIHH
                     GAEIACIRDLYAHSTNKKGN"
     gene            5684..6637
                     /locus_tag="VFC01_28665"
     CDS             5684..6637
                     /locus_tag="VFC01_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HZN83626.1"
                     /translation="MSETTSRVLQLLGLLQSRRVWTGEELAERLAVTTRSVRRDVERL
                     RELGYPVHASKGHGGGYQLGAGAALPPLLLDPDEAVAMAVCLRLAAGGSVAGVGESAL
                     RALSKLDQVMPARLRSQVAAVHEATVTLPSNYSDSPVEPEVLMTLARACRDHEHVTAG
                     YVSRVGDATERRLEPYQLVTTGRRWYLLAYDRDRQDWRSLRLDRMADVRALGSTFTPR
                     QAPDAATYVRRSISASPYRYVARVRYQAPEHVVAQHFSAASVTIEADGPDACIVTAGA
                     DDPERMVFYLALPGCEFEVLEPPEVVRAVGSLAGRLRRAAG"
     gene            complement(6634..7197)
                     /locus_tag="VFC01_28670"
     CDS             complement(6634..7197)
                     /locus_tag="VFC01_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83627.1"
                     /translation="MTILDVPARNDELRDALKRAASAFKAHGPEFALAGSYALWVHGG
                     PEPVHDVDFVVAEEDAEIAAKTLEEAGFRIDRPPEDWLFKACTGEVVVDVLHRLNGVP
                     VDPTTIRSADVLEVLAIAMAVLAPTHVVTEKLSSLNEHHCDFAALLPAVRAVREQVNW
                     DQVRRDTADHDFATAFLFLTDRLGITA"
     gene            7222..7989
                     /locus_tag="VFC01_28675"
     CDS             7222..7989
                     /locus_tag="VFC01_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZN83628.1"
                     /translation="MRLATFNILHGRSVHDGAVHTDRLVDSIKQLDADILALQEVDLD
                     QPRSGKADLTAVAAEAMGAVSHRFVAAINGTPGATWMAATGREQPGTAAYGIALLSRY
                     LAESWQVVHLPRIPMRFPMYLPGPRRVMVVHEEPRAAMIATIDTPIGPLTIANTHLSF
                     VPGWNRVQLRRLFRDLRGFPGPRVLMGDLNMTRPTPRRWTGMRPLGEAPTFPADGPCN
                     QLDHILTDDSSLRVDGCSSPRLPVSDHRALVVDVSRE"
     gene            8042..8329
                     /locus_tag="VFC01_28680"
     CDS             8042..8329
                     /locus_tag="VFC01_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83629.1"
                     /translation="MAMLEDLEARVAALEASQADYRAVLAAVNALGANQREQVLQLGS
                     VETRLTRVETDLGDFRIEVADFRQETRGRFRSVDEQLAEIKDLILGRRNGL"
     gene            8356..12543
                     /locus_tag="VFC01_28685"
     CDS             8356..12543
                     /locus_tag="VFC01_28685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HZN83630.1"
                     /translation="MHVISAESTELFTGPPDAPLQLVRVTHTGGAAEVRVEGEGLATP
                     TLAITDAAGTVEVAVSVSRPVPGEQRAARVLTGDVETPFVFTVAEPGWTMYMISHFHY
                     DPVWWNTQAAYTSVWSEDPPGRCRQTNGFDLVRAHLEMARRDPEYKFVLAEVDYLKPY
                     WDTDPEDREDLRRFIADGRVEIMGGAYNEPNTNLTSPETTIRNFVHGMGFQRDVLGAD
                     PSTAWQLDVFGHDPQFPGMAADAGLTSSSWARGPHHQWGPVAGGGDPRRMQFSSEFEW
                     IAPSGRGLLTHYMPAHYSAGWWMDSSATLAEAEQATYELFATLKTVALTRNVLLPVGT
                     DYTPPNKWVTDIHRDWNSRYTWPRFVCALPREFFGAVRAELSERGVEASPQTRDMNPI
                     YTGCHVSYIDTKQANRAAEDAVLDAERFAVFAGLLGGATYPQAALAKAWVQLAYGAHH
                     DAITGSESDQVYLDLLTGWRDAWELGTTARDNALQLLSSAVDAPVVVWNPLAHKRSDV
                     VTAHLTRPVGPGTRVFDCDGAELPTHVEHGGRSISWLARDVPSVGWQSYRLVSADAPA
                     GWKPLAGNEIANEHYRLRVDPVRGGGVSSLVEVDSGRELIADGRVGNELAVYDEYPAH
                     PQAGEGPWHLLPKGQVVSSSAEPASVQTYRGPLGERVVIRGRIGDVFRYTQTVTLWRD
                     VARVDCRTTIDEFTGADRLLRLRWPCPVPGALPVSEVGDAVIGRGFGLMHDGDAGEAV
                     DSAEHPWTLDNPAYGWFGLSSAARVRVGEEVRAVSVAEVVSPVESASASLARELMVAL
                     VRAGVTATCSSADKPRYGDLAVDSNLPDMRISLGGPDQNDFTAAVLADADPVYTEELK
                     RQLYSSATARVWVPPVSPLSEQWVPDADLRGVHALPVLIVAGADLDAAVAAVVEDLAD
                     FEIAVTQEAASDLDPFDDRTVAVLNRGIPGFAVDSDGTLHTSLMRSCSGWPSGTWIDP
                     PRRTAPDGSNFQLQHWTHSFDYALVSGDGDWRQADIPARSAEFSRPMLAVAEKTTAAG
                     GLPAWGSLLEIEPAGKVALGALKAAGNPLAAGSGQAVDPADGVAVRLVETCGATTDVV
                     VRSGLRRISAASRVDLLEQPRVQGLAPDGLTLHGYEIATVLTRLNMPQVLEADHTVLA
                     LDAEAVQPLYARYWLHNRGPAALGGLPAVAHLHPQHVEADPNSQVLLRLTVSSDSSDA
                     ALHGKVRVLCPGGWTAEPGELSFVLPPGEHLETDVEVTMAGDPPPGLYPLRAELAVTG
                     SDAVSMPPAWRQVVEDVCVVSVAAAEDADVLRLISDPEPVEVAAGETARLAVTVGTDA
                     HADLTVEAHLISPWGTWEWMGPAVVGREVPARGTVELGFDVAPPVWVEPGEWWALIRV
                     GCAGRLVYSPAVKVTVR"
     gene            12540..>12674
                     /locus_tag="VFC01_28690"
     CDS             12540..>12674
                     /locus_tag="VFC01_28690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl CoA-ACP transacylase"
                     /protein_id="HZN83631.1"
                     /translation="MSIAATVGGVPVSVEDVDAREERLRASRIASSLPRRGTSEGRQL
                     R"
BASE COUNT         1977 a         4085 c         4354 g         2258 t
ORIGIN      
        1 gtaacggatg acgcgcaggc cctggtcgac gagcttctcg cagaactccg tgcgccacaa
       61 cagaagttgc gcaccaagcc ccatgacgag caggacggcc gggtcgttgg gatcgcccat
      121 gtcctcgtag tagatgtcca gatcgccaga cttggcggtt ccggtgcgaa cctgcactta
      181 cgcctcgact tcctcggtgt gctcgcggga tacctcgacc atgaagttgg cgaagtatcc
      241 ggtcagctgc gggtcggaca tcatctgcca cttcggagcc agcagcttca tgtagcgctc
      301 gacgtagagg aactgcttgc cgatcagcac cagctcgcgc ggcagcttga cgtcgtaggc
      361 gtcggccagc gtcgagagct ggcgaccgat gtcggcatag ctcatgtcgc ccagcgtcga
      421 catggtcagc ggggtggcga acttctccag atccttcgcg gcctgtgcct cgggtttgac
      481 ggtgccgacc gcgcccatca tcacgacgat cttgcccgcc gtggcgtggt ccttcttgac
      541 cagcagcgcg tacaccagct cccttaagag ccaccgcgtg cgcgggtcga tgcggcccat
      601 gatgccgaag tcgaagaaca cgatcttgcc gtcggcgtcg acgtacaagt tccccgcgtg
      661 caggtcgccg tggaagaggc cgtgccgcag cccgccctcg aagacgctga acagcagggc
      721 cttcaccagc tcggtgccgt cgaagccggc cttgcggatg gcagcggcgt cgtcgatgcg
      781 gatgccgccg acgcgttcca tcgtcagcac ccgctcactc gtcaggtccc agtagacctg
      841 cggcacgcga atgttgcggc ccagcggcga ggcgtgcatg tgcgacaccc aggcgtccat
      901 cgactgtgcc tcgagccgga agtcgagctc ctcggccagg ttgtcggcga agtcggcgac
      961 aacgtcctgc gcggacagcc gccggcccag cttggccagc tcgacgatct gcgccccgcg
     1021 cttgaggatc tgcaggtccg cggcgacgcg ccggcggatg ccgggccgct ggatcttgac
     1081 gacgacctcc tcgccgctgt gcagggtcgc gtagtgcacc tgcgcgatcg acgcggacgc
     1141 gaacggcgtg tcctcaaacg tcttgaacag gctcttcggc tcgtcgccca gctcttcggt
     1201 taacagcttg tgcacttcgt cggggtcggc gggcgggacc gcgtcgagca ggctgcggaa
     1261 ctcgcggctc atgtgttcgc cgaacgcgcc cgggctggac gcgatgatct ggccgaactt
     1321 gacgtaggtg ggcccgagat cggagaaggc cttcgggatc tgcttgatga tcttctgctg
     1381 cagcgagccc ttaccggcga gggtggagac gacccgtgcg ccggtgcggg ttatctgcca
     1441 acccgtcgcg ccgatgcggg cggcctcgac cggcaacggc acccggtcca gcttggccac
     1501 ctcgcggtgc ttagtcgtac tcatcgtgcc agtgtctcaa aacccggcgt gatgcttcga
     1561 atccgtgtgc accgggtcac acagcgctcc gcggctgagc acaatgcgca gaacctcaat
     1621 atcgcttgtt acacggctag tgcacacaag cgcgccatcg tcgccgccga tccatagctt
     1681 cggacgcaat gaacgtttga ggagcgaaaa tggcgacgct ttcgatcgaa ccgcagcgca
     1741 cctcgatgag gcgcgtcgcg gccgccagct tcgtcgggtc cgccatcgag tactacgact
     1801 tctacatcta cggcaccgct gccgcgctgg tgtttccgaa ggtgttcttc ccgcacctgg
     1861 gcacgaccgt ggggaccgtc gcgtcgatgg ccacgttcgc cgcagcgttc ctgtcccgtc
     1921 cattgggtgc cgcgttcttc gggcacttcg gcgatcgact gggtcgcaag tcgaccctga
     1981 tcgccacctt gctgatcatg ggactgtcca ccctggccgt cggtctggtt ccgggtgcgg
     2041 tgtcgatcgg tatggctgcg ccactgatcc tgttgacgct gcgcctggtg caggggttcg
     2101 cggtcggcgg cgaatgggcc ggcgccgcgc tgctgtccgc tgagtacgca ccggcgcaag
     2161 cccgcggccg ctacggcatg ttcacccaga tgggcgtcgg cagtggcctc gtcatgagca
     2221 gcctgctgtt cctcgtggtg aaccagacga tcggggagtc cagtcgcgcc ttcatcgatt
     2281 ggggttggcg gataccgttt ttgttcagtg cggtgttgat cgtcatcgct ctatacgtcc
     2341 ggctcaatgt tgccgagacg cccgtattcg ccgaacagaa ggcgcgcgat accgccaggg
     2401 tgatgccgct ggctgccttg ttcagaagcc agcggcgcga ggtggttctt gccgccggaa
     2461 gcatgatcgg cttcttcacg ttgggctaca tggccaacgc atacctgatg agttacgccc
     2521 atacccacgt tggattttcg ccgaagctga tcttgtcggt gggcctgctg ggcggtgtgg
     2581 tcgtggtggt cttcaacgtg ctgggcgccg tgttgtctga cgccattggc cgccgtcgcg
     2641 tcatcatgac ggcgttcgca atcggtgttc cgtggtcgtt cgttgtgctt ccgctggtgg
     2701 acacaggcaa tgctgtgctg ttcgcgctgg cattggcagg cacttatgcg gtcgcgggaa
     2761 ccgcctacgg cccgatggcg gcattcatcc cagagatctt cggcacccgt taccgctaca
     2821 gcggcgccgg tctgtccctc aacctcgcgg ggttggtcgg cggcgcagtg ccgacgatcg
     2881 tcgccgcccc gctgcaggcg acgtggggcg tggccgcggt cggcgccatg atggccgtcg
     2941 tcgttgtggc cagcctagtg tgcaccgccg ccctgcctga aacgaaaggc gctgcgttgc
     3001 aacgcagttg agcacaatgc gcgaaacgcg gagatcgccc aatgcgcggg tcgtgagcac
     3061 aacagcgcca cggtgagcgc ggatgggtag tttcggtggt gtgacaaggt cacaaacaac
     3121 caaacatcaa ctctgacagt agaattcaaa tgaacactaa taacaagtcc cgcatcttca
     3181 cccggttcgt tgcgggtccg atcgccgccg cgggcattct gggcggtgtc gcaatcggat
     3241 tggccgcagg cgccaatgca gaccccggtc cgtcggatca cccgagcggc ggctactcgt
     3301 cggagttccg ctccggccac gattcgcgtg attcgtaccg ggacttccgg cccgacttct
     3361 gggacttctg gttctggcac caccaccggg atcactcgtt cgggtggcac cgcgacagct
     3421 gatccgtgtt gtctcccaca cactgagccc cttctcgact tatcgggaag gggctcagtg
     3481 catttggcga tatcaggcca tgttctccgt tagcgcctcg acgacgggcc gccacaccgg
     3541 ctcgggcagg tcatggccca tgccgtcaac gacgacatag cgtgcgccgt cgatggcgcg
     3601 agcgacattg cgtccgttgc ggggccgcac catcggatcc ttcgagccgt ggacgacgac
     3661 cgtcggcgca gtgatacgtc gggtgtagcg catcaggctg ccggtgccca ggatggcgtc
     3721 gaactggcgc agcatgccct gcttgtagtc gctgcgggcg gtcagctcct cgacgcggcg
     3781 gcgcaactgg tcaacgggcg gaaggaaatt cgggccgttg atgatcgaga tgttgcgcac
     3841 ctcggtcgcg agcttgtcct cgacgggcgc gttctttggt ggagcgttga acgccaacct
     3901 gatcacccgc cacttcggga tcgacgacat cggcgtgcct gcgctcgtca tgatcaggcc
     3961 cagcgaccgg acccgagcgg cgtgggcgcc ggccagcacc tgggcgatca tcccgcccat
     4021 ggacgcgccg acgacgtgcg cgcgctcgat cccgagatgg tcgagcaggc ctttgacgtc
     4081 ctcggccagg tcgaccagcg tgtacggcac cgggctgctc cggccgacga catagcgcag
     4141 cactcgcgga tagaccgagc ccttggcccg cgttccgtgc atcttggtcg acaggccggt
     4201 gtcgcggtgg tcgaagcgga tcacccggta accgcatttg accagctggg cgcagaaacc
     4261 gtcgggccac atcggcagtt gtgcgccgac gcccatgatc agcaggacag gcgggtcggc
     4321 agggtcgccg aggtcttcgt agaagagctc gacgtccccg cacgccgccg taccgcttcg
     4381 ggtctgcacg tttccctgtt taccccgtca ccgagcgcac ggttgtgtac gcattcgtcg
     4441 agtatggacg taaacaaccg tgcggtcggc gatgaactag acctgggccg gcttccaccg
     4501 cttgaggaag tcggtctccg gccactcctc gttggcggcc atcagctcgt acatcgttgc
     4561 gccgtcgatg gtttcgcgaa tgatgtcggc atgcccggcg tggcgcgtca gctcctcgat
     4621 caggtgaagg gccacccagc gcacactcca gttctcgaca tcttgcggga accacggcac
     4681 ctcgtgcggg actggaaccg gggtgtccag atcggcttct gcgaagacgc gtagggtctc
     4741 ctcgttctgc ttctccagcc tgtcgagcag ttcatcgagg gtttcgtcgt cacgcatcgt
     4801 gtgctcgtcg gcgtaggagg ccatgatctc ctccatgggc cggtcgtctt tcggcgggaa
     4861 gtccggggcc gcggccgccc gctccaccca gcccttctgc acgccggcgg cgtgcttgac
     4921 cagtccgccg atcgacagcg cactcgccgc aggggctgac ctggcctgct catcggtcag
     4981 gccgtaggcc gccgagaaga acgcgtcctg gttgaaccgg aggaactcga tcaatgactg
     5041 ccgctcaccg gaggcgggtg ggggcgttcc tggcatcggt cagtttccct tcttgttggt
     5101 ggagtgtgcg taaagatctc gaatgcaggc gatctcggcg ccgtggtgga tggcctccct
     5161 gttgatgtgc aggacgagag caatgaacgg atagtcagcg taaggtcctt cggacggacc
     5221 acacggctgc tccaagtcat cttcggacag gcctcgcact ccggcgatcc attttgcata
     5281 ggcggcgtcg agctgttgca gagcggtctt ggcatcagtg gcgtagttcc aggattggta
     5341 gtcggccggc ggcccgccaa agtgcgagtg gttgcgcatc tcgaagacgc cgacgatgac
     5401 gggggcgagc cgccacgcga tagtcgtgaa cggcaccggc tgcggctccg ggtaggtgaa
     5461 gtcgatcgag ccgtcggggt gaaccgtcca gcagtccggc accggctgcc agaagtactc
     5521 ctcatcggtc aggccctcca gccgcggacg cagctggttc gtccagtgcc agtcgagttg
     5581 ctcggccagt cggaaggtga ggtctttcgt atcggtgctc atgagtacag actgtcagca
     5641 atataggaca gtttttgtcc tatgactgtg tcagggttgt tgggtgtccg aaacgacgag
     5701 ccgggtgttg cagcttctcg ggctgctgca gtcgcgccgg gtgtggacgg gtgaggagct
     5761 ggccgagcgg ctggccgtga cgacgcgcag cgtgcgccgc gacgtcgaac ggctgcggga
     5821 gctcggctac ccggtacacg ccagcaaggg ccacggcggc ggctatcagc tgggcgccgg
     5881 cgccgcgctg ccgccgttgc tgttggatcc cgacgaggcg gtcgcgatgg cggtgtgtct
     5941 gcggctggcc gcaggcggaa gcgtcgcggg cgtcggcgaa tcggcgctgc gcgcactgag
     6001 caagctcgac caggtgatgc ccgcgcggct gcgatcgcag gtggcggccg tgcacgaggc
     6061 gacggtgacg ctgccgtcga actactcgga ctcacctgtc gaacccgagg tcctgatgac
     6121 actggcgcgg gcctgccgcg accacgagca cgtcaccgcc ggctacgtct ctcgggtagg
     6181 ggatgccact gagcggcggc tggagcccta tcagctggtg acgacgggcc gccgctggta
     6241 tctgctggcc tacgaccgtg atcggcagga ctggcgcagc ctgcggctgg accgaatggc
     6301 cgatgtgcgg gcgctcggca gcacgttcac gccgcggcag gcgcccgatg cggcgaccta
     6361 cgtgcggcgc tcgatcagtg cgtcgcctta tcgctacgtc gcccgggtgc gctatcaagc
     6421 gcccgaacac gttgtagcgc agcacttttc cgctgcatca gtcaccatcg aggcagacgg
     6481 tcccgacgcg tgcatcgtga ccgccggcgc cgacgatccc gagcgcatgg tgttctatct
     6541 ggcgctgcct ggctgcgagt tcgaggtgct ggagccgccg gaggtggtcc gggcggtcgg
     6601 atcgctggcc gggcggctgc gccgggcggc cggttaggcg gtgatcccga gccggtcggt
     6661 gaggaacagg aaggccgtgg cgaaatcgtg gtcggcggtg tcgcgacgga cctgatccca
     6721 gttcacctgc tcacgcacgg cgcgcacggc aggcagcagg gccgcgaaat cgcagtgatg
     6781 ctcgttgagc gacgacagct tctcggtaac cacgtgggtc ggcgccagca ccgccatcgc
     6841 gatcgccagc acttcgagca cgtcggcgct acggatggtg gtcgggtcga cgggcacacc
     6901 gttgagccgg tgcagcacgt cgacgaccac ctctcccgtg cacgccttga acagccagtc
     6961 ctccggtgga cggtcgatcc ggaagccggc ctcctcgagc gtcttggctg caatttcggc
     7021 gtcctcctcg gcgacgacga agtcgacgtc gtgcactggt tccgggccgc cgtgcaccca
     7081 gagcgcataa ctgcctgcga gcgcgaactc ggggccatgc gccttgaagg ctgacgcggc
     7141 gcgcttcagg gcgtcgcgca gctcgtcgtt tcgggcgggg acatccaaaa tcgtcatcgt
     7201 gggtaagtaa tacccatgca gatccggttg gccaccttca acatcctgca cggacggagc
     7261 gttcacgatg gcgccgtaca cacggacagg ctcgtggact cgatcaagca gctcgacgcc
     7321 gacatcctcg ctctgcagga ggtcgacctc gatcagcccc gctcgggcaa ggcggacctg
     7381 accgcggtgg ccgccgaagc gatgggcgcg gttagccacc gtttcgtcgc ggcgatcaac
     7441 ggcaccccgg gggcgacgtg gatggccgct accggtcgag agcagcccgg aaccgcggcc
     7501 tacggcatcg cgttgctgtc ccggtatctc gccgagagct ggcaagtcgt gcacttgccg
     7561 cgcattccga tgcggttccc gatgtatctg cccgggccgc ggcgtgtgat ggtcgtacac
     7621 gaggagccac gggccgcgat gatcgcgacg atcgacaccc cgatcgggcc gctgaccatc
     7681 gccaacaccc acctgtcatt cgtgcccggc tggaaccggg tgcaactgcg acggctattc
     7741 cgcgacttgc ggggcttccc gggcccacgc gtattgatgg gcgacctcaa catgacgcgg
     7801 ccgacgccgc gccgctggac cgggatgcgg ccgctcggtg aggcgccgac gttccctgcc
     7861 gacgggccct gcaaccagct cgaccacatc ctcaccgacg acagctcgtt gcgggtcgac
     7921 ggatgctcgt cgccgcggct gccggtgtcc gatcatcggg cacttgtggt cgacgtttcg
     7981 cgggagtgag gcctgtggat aaccgggagc acggcggcgg cgcggccaat acgatgagtg
     8041 ggtggccatg ctagaagacc tcgaggcgcg cgttgcggcg ctggaagcct cgcaggccga
     8101 ctaccgcgct gtgctggccg cggtgaacgc attgggcgca aaccaacgcg agcaggtgct
     8161 ccagctaggt tcagtcgaga cgcggctgac gcgggtggaa acagacttag gtgatttccg
     8221 catcgaggtg gccgacttcc ggcaggagac tcggggcagg ttccgctcgg tcgacgagca
     8281 actcgccgag atcaaggatc tgatcctcgg ccgtcgcaac ggtctctaac gcgtcgacgt
     8341 cgtagtgttc ttggcgtgca cgtcatctcc gcggagtcga cggagttgtt tactgggccg
     8401 ccggacgcgc cgctgcagtt ggtgcgggtc acccataccg gcggtgcggc ggaggtccgt
     8461 gtcgagggcg aggggctggc cacaccgacg ctcgcgatca ccgacgccgc cgggacagtc
     8521 gaagtcgccg tgtcggtttc gcgtccggtc cccggcgagc agcgggccgc gcgggtgctc
     8581 accggtgacg tcgagacacc gttcgtgttc accgtcgccg agcccggctg gaccatgtac
     8641 atgatcagcc atttccacta cgacccggtc tggtggaaca cccaggctgc ctacaccagc
     8701 gtgtggagcg aggatccgcc cggccggtgt cgtcaaacca acgggttcga tctggtgcgc
     8761 gcccatctcg agatggcgcg ccgcgacccg gaatacaagt tcgtactggc cgaggtcgac
     8821 tacctcaagc cgtactggga caccgatccc gaggaccgcg aggacctgcg ccggttcatc
     8881 gccgatggcc gcgtcgagat catgggcggc gcgtacaacg agcccaacac caacctgacc
     8941 agtcccgaga cgacgatccg aaactttgtg cacggcatgg gttttcagcg tgacgtgctg
     9001 ggcgccgacc cgagcaccgc gtggcagctg gatgtgttcg ggcacgaccc gcaattcccg
     9061 gggatggcgg ccgatgccgg gctgacgtcc agctcgtggg cccgcggccc gcaccaccag
     9121 tggggtcctg tcgctggcgg cggcgatccc cggcgcatgc agttctccag cgagttcgag
     9181 tggattgcgc cgtccggccg cggtctgctg acgcactaca tgcccgcgca ctactccgcg
     9241 ggctggtgga tggattcgtc ggcgacactg gccgaggccg agcaggcgac ctacgaattg
     9301 ttcgcaacgc tgaagacggt cgcgctgacc cgtaatgtgc tgctgccggt cggcactgac
     9361 tacacgccgc cgaacaagtg ggtcaccgac atccaccgcg attggaactc gcgctacacg
     9421 tggccgcggt tcgtgtgcgc tttgcctcgg gagttctttg gcgccgttcg cgcagaactc
     9481 tcggagcgcg gcgtcgaggc gtcgccgcag acccgggaca tgaacccgat ctacaccggc
     9541 tgtcacgtgt cgtatatcga caccaagcag gccaaccggg cagccgagga cgccgtgctc
     9601 gatgccgaac ggttcgccgt gttcgcgggc ctgctcggcg gagcaactta tccgcaagcc
     9661 gcactggcta aagcatgggt gcaactggcc tacggcgcac accacgacgc aatcaccgga
     9721 tcggaatccg atcaggtgta cctcgacctg ctgaccggat ggcgggacgc ctgggagctc
     9781 ggcaccacgg cccgcgacaa cgcgctgcag ctgctgtctt cggccgtgga cgcgccggtc
     9841 gtggtgtgga atccattggc gcacaaacgg tccgatgtag tgaccgcaca tctgacccgt
     9901 ccggtcggcc cggggacgcg ggtgttcgac tgcgatggtg ccgagctgcc aacgcacgtc
     9961 gagcacggcg ggagatcgat cagctggctg gcccgcgacg tgccgtcggt gggctggcag
    10021 tcctaccggc tggtctcagc ggacgcgcct gccggttgga aaccactggc gggcaacgag
    10081 attgcgaacg agcactatcg gctgcgggtc gatcctgtac gcggaggcgg ggtcagctcg
    10141 ctagtcgagg ttgactcggg gcgcgagttg atcgccgatg gcagggttgg caacgagttg
    10201 gccgtctacg acgagtaccc ggcgcatccg caggccggcg aaggcccatg gcatctgctg
    10261 ccgaaggggc aggtcgtctc ctcgtccgcc gaaccggcct cggtgcagac ctaccgcggg
    10321 ccgctgggcg aacgggtggt gatccgcggc cgcatcgggg acgtgtttcg ctacacgcag
    10381 accgtgacgc tgtggcggga cgtggcgcgc gtcgactgcc gcaccaccat cgacgaattc
    10441 accggcgccg accgcctgct gcggttgcgc tggccttgtc cggtgcccgg cgcgctgccg
    10501 gtgagtgaag tgggcgacgc cgtcatcggc cgaggtttcg gcttgatgca cgacggtgat
    10561 gctggcgagg ccgtcgactc agcggagcac ccgtggaccc tggacaaccc ggcgtatggc
    10621 tggttcgggt tgtcctcggc ggcgcgtgtg cgcgtcgggg aagaggtgcg agcggtgtcc
    10681 gtggccgagg tggtgtcgcc ggtggaatcg gcgtcggcgt cgttggcgcg tgagctcatg
    10741 gtcgcgcttg tccgcgccgg tgtcaccgcg acgtgcagca gcgccgacaa gcccaggtac
    10801 ggcgatctgg ccgttgactc caacctgccc gacatgcgga tctcgctcgg cggcccggac
    10861 caaaacgatt tcaccgcagc agttctcgcg gacgcggacc ccgtgtacac cgaggagctg
    10921 aagcgacagc tgtattccag cgccacggcg agggtgtggg tgccgcccgt ttcgccgttg
    10981 tccgagcaat gggtacctga cgccgacctg cgcggcgtgc acgctttgcc ggtattgatc
    11041 gtcgcgggcg cggacctcga tgccgcggtg gccgccgtcg ttgaagatct cgccgacttc
    11101 gagatcgctg tcacgcaaga ggcagcgtca gacttggacc cattcgacga ccggaccgtt
    11161 gcggtgctca accgcgggat tcctggcttc gcagtcgatt ccgacggcac gctgcatacc
    11221 tcgttgatgc gctcgtgcag cggctggcca tccggtacct ggatcgaccc gccgcggcgc
    11281 accgcccccg acggctctaa ctttcagctg cagcactgga cgcattcctt cgactacgca
    11341 ctggtctcgg gcgacggaga ctggcgccag gccgacatcc ccgcccgcag cgccgagttc
    11401 tcgcgtccga tgttggcggt ggccgaaaag acaacggcgg caggcgggtt gccggcatgg
    11461 gggtcgctgc tcgagatcga gccggccggc aaggtggcgc tcggcgcatt gaaggccgcg
    11521 ggtaatccgc tcgcagcagg cagcgggcag gcggtcgatc ccgccgacgg cgtcgcggtc
    11581 cggcttgtcg aaacctgcgg cgccaccacc gacgtcgtcg tccggtccgg gcttcgtcgt
    11641 atctcggcgg cctcgcgcgt cgacctcctc gaacagcccc gggtccaggg actcgcaccg
    11701 gacgggttga ctctgcacgg ctatgagatc gccaccgtgt tgacacggct caacatgccg
    11761 caggtgctcg aggcggacca caccgtgctc gcgctcgatg ccgaagcggt gcagccactc
    11821 tatgcgcggt actggctgca caaccgtggt cccgccgcgc tgggtgggct gcccgccgtc
    11881 gcgcatctgc atccgcaaca cgtcgaggcc gatccgaatt cgcaagtgct acttcggctt
    11941 accgtgtcca gtgacagcag tgacgccgcg ctgcatggca aggtgcgggt gctgtgccca
    12001 ggcggttgga cggccgaacc gggggaactg tcgtttgtgc tgccgcccgg cgagcatctg
    12061 gagaccgacg tcgaggtgac gatggcggga gacccgccac cggggctgta tccgctgcgc
    12121 gccgagctgg ccgtgacggg aagtgacgcc gtgtcgatgc ctcccgcgtg gcgtcaggtc
    12181 gtcgaggacg tgtgcgtggt gtcggtcgct gccgccgagg atgccgacgt gcttcggttg
    12241 atcagtgatc ccgaacccgt cgaggtggca gcgggtgaga ccgcgcggct cgcggtcacc
    12301 gtcggcaccg atgcccatgc ggatctgacc gtcgaggcac acctgatcag cccgtgggga
    12361 acatgggagt ggatggggcc agccgtcgtt ggccgcgaag tgcccgcccg cggcacggtg
    12421 gaactcgggt tcgacgtcgc gccgccggtc tgggtggaac ccggtgaatg gtgggcattg
    12481 attcgagtcg gctgtgccgg ccggcttgtc tattcgccgg cggtaaaggt gacagtgcgg
    12541 tgagtatcgc ggcgacggtc gggggcgtgc cggtgtcggt cgaggatgtc gacgcccgcg
    12601 aggagcggct gcgcgcatct cggatcgcgt catcattgcc gcgacgtggc accagcgaag
    12661 gcaggcaact gcgt
//
LOCUS       DASRJA010000641        19792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3464608,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000641 DASRJA010000000
VERSION     DASRJA010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 19792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19792
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3464608"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFC01_28695"
     CDS             <1..366
                     /locus_tag="VFC01_28695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83632.1"
                     /translation="KFMPTALAAAAIGLGALMTVGAPSAHASEKSDCEGKGGTYTETQ
                     IYNQTTGKSGTNYRCCVKDTATNTTTCTSTTVTKNVRIPVTVRPGVIDTAETVEPAQP
                     IQRVPVDVLNTDAQIQQTP"
     gene            407..532
                     /locus_tag="VFC01_28700"
     CDS             407..532
                     /locus_tag="VFC01_28700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83633.1"
                     /translation="MSETTRYNLENDAVDWRDLADQIFPADALRTAPTVHPLAAV"
     gene            702..2795
                     /locus_tag="VFC01_28705"
     CDS             702..2795
                     /locus_tag="VFC01_28705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN83634.1"
                     /translation="MSVVEAISFGHYRLTDLLGRGGMGDVWRAHDTRTDRIVAVKLLP
                     RKLAHDDIFKQRFRREARAAAQLNEPHVVPIHGYGEIDGQLYVDMRLIEGRDLHDLLT
                     TGPLKPARAVMIIDQVAKALHAAHNVGLVHRDVKPSNILVADYDFAYLIDFGIARGAG
                     EATLTSIGTWVGTPYYMAPERFRDGPVDARADIYALACVLHECLTGTTPFPGSSMESQ
                     MTAHLTMPPPRPSVVVRGVPATFDAVIAKGMAKDPDQRYRTVLDLAAAARSASSTPIP
                     TLPPPPPSEEPTVREATLYAGDEDATVRFGEPGKPRPPRNRRRLAMIGAAPTVVVLAL
                     VAAVSLWVGRDKPGVSGPEIFLEPAMALGQNPFTPLASRPQPPATFPGSDPVGAPVSG
                     GTDPDRGPVSGGQVRAVRGDVPGLYGGTRNLSPCDTGLMTSSLGQNPDRGQAWAATFN
                     ITAAQIPAFLSGLTPVLLRTDTRVTSHGFVDGQASPYQAVLEAGTAVLVNNFGVPVVR
                     CGGGNPLLEPVAMTSTRYSGERWKSFTIGAVTVIRPSDNPVDAFILYDSTSGDSFARP
                     LGGNGSNDFPDPRVPTTSTLPRPPLETQTQAPPPPPMTRVTDFPTYAPPSITYAPPIP
                     TQLPRATTATTAPRETSSAPRTSYYKPPTTTLPRRDRSGVGGSTGTPSQKPPPSGSKA
                     PEPTAPAPKPPPKPL"
     gene            complement(2828..3484)
                     /locus_tag="VFC01_28710"
     CDS             complement(2828..3484)
                     /locus_tag="VFC01_28710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003916736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppA family lipoprotein"
                     /protein_id="HZN83635.1"
                     /translation="MRRRHRMVGVLIAVLLSVALPSCVKPSTFNPAAAPDNSELDRLQ
                     KILNDRPDLEVAEQQLIDLDAQIRSVIAKYSAATVVGPSTPKPDRGCVDPFTHNIGDT
                     YTIEKIYARPAPTDEQWTQITDALTPIFAAAKFTPNVPPNTAPAPGTFSQIRDDGATV
                     ELINSPNTVLFYGYSTGCRLPAAWRTAPPPPDQRPANDSGVHYPYLYGSPGGRSAPAN
                     "
     gene            complement(3481..5184)
                     /locus_tag="VFC01_28715"
     CDS             complement(3481..5184)
                     /locus_tag="VFC01_28715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83636.1"
                     /translation="MTVSIADIDRWDPGDVREVFHATRNRAEAAFEAADGIATLPAFG
                     SWGGDAAEAAKTANEQLRKDLDAHGNEALAVAKAAQRAADDMEQVKSDLAQLEADAAG
                     AGFEIDRASNAVMPGPALRGSMVDLIAAEGKREELQARLTAILAEAVRVDEELAQAIN
                     MATGATPIPDEKPAGPPLPQPGTEPADVKKWWDSLSNEQRQELTRTHAAEIGNLDGIP
                     AAVREQVNAARLPEEIAKAQALVDYMRAHPNGKPNPAQLAAWQGARDKLADLQQLDKT
                     LKDHPGVGLLLLDTTSNPKTVLAAVAKGDVDNARQVGVTVGGMTTNVRGSVDGMTGEI
                     ITQYDQAVELRQLAGVANPESVATIAWLGYEAPGMNFDVTDDALAKAGAGPLNNFYQG
                     LGATSNVPDQEITAFGHSYGSLTTSLALQQGAPVDNVVLYGSPGAELSNASQLGVAPG
                     HAFYMNGITDGVPTTIAGTHNFGPPLSEVPGFTELSSNSGQALPPPYGDGQWHERAYG
                     HSEYPRLGSNGELRMSGYNMAAILAGLPGETIAPPQNLPPPTLPLGPHGMPVPNPNYH
                     P"
     gene            complement(5197..5526)
                     /locus_tag="VFC01_28720"
     CDS             complement(5197..5526)
                     /locus_tag="VFC01_28720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83637.1"
                     /translation="MSGWIRVAAEDLHVSAATVDVHADDLHLRHATANGRIEGAQVGV
                     PMGSAAALSAAVAKWQADSTVLFGNLVGHGEALRGAAARYMGTDEDKAAAIEAAGDQA
                     TTLDLGL"
     gene            complement(5707..7737)
                     /locus_tag="VFC01_28725"
     CDS             complement(5707..7737)
                     /locus_tag="VFC01_28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83638.1"
                     /translation="MTNLLNPFLAPAPNTPDPATPVIWAVLGWVRRNFFNQAPTITYN
                     PGTTTQTGQTVIGYIGAKDPENDALTYTVTKAPAHGTLTIDQATGKFAYTPDEIDYDS
                     EQRVSFTVSVTDGKFNLLSLFKPHSDEETIEFKVQPPTVERVILNMPDGVKNPANPRY
                     SEDGKSIYFAGTPTAGGRSEIYKISIDGTHTECVTCGVAGPTIATSDPSLPVNLRKPV
                     PFYDGSGRVVVLLDNPEPRYAIFEPAGYNGIGSPARIVNVITPDGGGATLPGLPFPGG
                     IVNREREMRPSPDGTHVLFTRIVFGQTGGLQALPIVGELTQNGDHYDVTNARVVWPTG
                     EGKQWTPDGKGVVIQGGAIDAGNIDDIMVDLSGQTGDNLYPGSPFRGTRVSGNLDYDE
                     DMDFSPNMQWIAIGSTRGFDALTPMTRIVRQNFLPVYVTAIYAQYADDQVVNVSNQNW
                     AVALEDDRDRENGIPLFVQDDPTTPEVDEGDGWASRSMPSWNADGTAVTFWESGNGDE
                     HGVAPTESRIVIANLKYTTSVGPVGDTTTVFDPSAFQDLTTYVAKTTPLPPTGTYSGV
                     GGGTAVVSESFDPTTRQTTRTVVYTKYVNEDGLILTGTEKAIYGPSQSNVTYLADIDV
                     TDATGANRGKLEANAVVSPFAQTVTGYITSTLDGDTQTIPDLAKAEAAKSGA"
     gene            7778..8068
                     /locus_tag="VFC01_28730"
     CDS             7778..8068
                     /locus_tag="VFC01_28730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83639.1"
                     /translation="MTRAAAAVGAAAAAIDWVVSVLELLTVNACAVGTALDLVVVADG
                     CATVATTFAAPMEVVGDDGAVASLTVAGCVELDPVLAEVVCSGAGVAGGVLG"
     gene            8093..8437
                     /locus_tag="VFC01_28735"
     CDS             8093..8437
                     /locus_tag="VFC01_28735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83640.1"
                     /translation="MAGSVEFGWVSTTLVDVGAFDDVDSVVLVCAPPLLLMVTPGATS
                     VDEDVALLVEVDPPLVGVDPVPVVAVDEVAVPVVDVAAGPVPDSPDDVELAELVEDES
                     EDVPVVSAAANP"
     gene            8691..9290
                     /locus_tag="VFC01_28740"
     CDS             8691..9290
                     /locus_tag="VFC01_28740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN83641.1"
                     /translation="MATARKAKADDGGTRRRLLDATAQIMHDEGYAAATSRRVAAKAG
                     IRQPLVHYYFPTMDDLFLAVLRAGADVALDQMRAALTDNNPLRALWVLNSDPRRSVLN
                     TEFMALANHRKAIGVELRAYAERVRDMETAAVTLVLRAHGVDLNAYPPVVISMLIAQI
                     ARSLCNEDAVGVTLGHDEMRRFVEQQIDALAEPSTVSGS"
     gene            complement(9426..9812)
                     /locus_tag="VFC01_28745"
     CDS             complement(9426..9812)
                     /locus_tag="VFC01_28745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83642.1"
                     /translation="MESRPVWRELGVITGYDWIISGGMDSLLATLQHQPVLVGSYWFE
                     DMMTTGERGVITSACDPPLEDAGGHEYLANAILWPDNPRRRLIGFEQSWGEEPEDFAP
                     TFYMGWDLVEHLVFGLAGDVAVPRLL"
     gene            complement(9838..10575)
                     /locus_tag="VFC01_28750"
     CDS             complement(9838..10575)
                     /locus_tag="VFC01_28750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83643.1"
                     /translation="MSEANEDGYISPECFRAYVSAEIKVPNTLIAVGPYVGRAALASL
                     RPGDYFQPAIKRFATTYAFSPSQVKSAVRTVVLMHRRFGVPVLPESADLQGDNAIRLF
                     KHQRFEVVYMEGVMNILEAILRLHGEQGTKGCPRDSRVAELLEHPDKLAATVAAFAAA
                     ETAINSPAATERSEAFKQIFNFVKSGEKPRPMAPADKDGIQAEVMPLLVAAVSEALAE
                     CKSTTSHDQTEEFLRILTSPELHEEAA"
     gene            complement(10590..12038)
                     /gene="guaD"
                     /locus_tag="VFC01_28755"
     CDS             complement(10590..12038)
                     /gene="guaD"
                     /locus_tag="VFC01_28755"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HZN83644.1"
                     /translation="MPASCAIRGSFFDFVDDPWKHPGHEDRAARFVGDGLLVVKDGII
                     EDYGHFADLSRRHADLPVTHLQDRLILPGFIDGHIHFPQLRALGVFGGQLLDWIQTWI
                     FPEELKYKDRSYARTAAGYFFDALLAGGTTTAMAFTTSFPVSTEEFFDEATRRGMRVI
                     AGLAGIDQYGPDDFLITADDFYRESKRLIERYHRTGRNLYAITPRFPGVCSSEMLERC
                     RQLKQEHQDCWINTHISENPSEVRMVRSEFPDCQDYTAVHASHGLLGPKFVAGHGVWL
                     SRDEFRRFSAAGATIAFCPLSNLFLGSGLFPLGRALDPEHPVRVALGSDMGGGNALSI
                     IRVLEEAYKVGTCNNTMLDGSVDPRDQDPAEAERNILSPYRAYYLATLGGARALYLDD
                     LLGNFAKGKEADFVAIDWNAGQAAMRWRQSLIVEGAPETVDQAAQLLFGVMMVGDDRN
                     VDETWISGKRAYKSDAVAHTDLRASDHTAQGL"
     gene            complement(12154..14946)
                     /gene="ppc"
                     /locus_tag="VFC01_28760"
     CDS             complement(12154..14946)
                     /gene="ppc"
                     /locus_tag="VFC01_28760"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HZN83645.1"
                     /translation="MFFAPLAARAVQRTRVGREASEPMRADIRLLGTILGDTVREQNG
                     DEVFDLVERARVESFRVRRSEIDRAEMSRIFDGIDIHLAIPIIQAFSHFALFANVAED
                     IHRDRRRGIHVAGEPPQDGSLAAAYAKLDLAELDSSSVADALQGALVSPVITAHPTET
                     RRRTVFVTHHRITELMRQHAEGRTETNDGRSIELELRRHVLTLWQTALIRLSRLKITD
                     EIAVGLRYYAAALFEVLPQVNADVREALRARWPDADLLAEPILRPGSWIGGDRDGNPN
                     VTADVVRLATGSAAFTALARYLAELTDLERELSMSARLITVTPEVAALADVCTDTVDL
                     DEPYRRALRTIRGRLTATAAEILDHQPQDGLDLGLPPYGTPAELRTDLDAIDASLRTH
                     GAALLADDRLAHLRESVHVFGFHLCGLDMRQNSDVHEEVVGELLASAGVHPDYGSLPE
                     DKRVELLTSELSTRRQLVSDRAQLSQLARGELDVVAAAAHAVEVYGAAAVPNYIISMC
                     QSVSDVLEAAILLKEVGLLDASGAEPYCPVGISPLFETIDDLHNGALILHAMLELPLY
                     RSLVDARGGCQEVMLGYSDSNKDGGYLAANWAVYRAELTLVEVARKTGIRLRLFHGRG
                     GSVGRGGGPSYQAILAQPPGAVNGSLRLTEQGEVIAAKYAEPQLARRNLESLVAATLE
                     STLLDVEGLGNGAEPAYGVLNEVATLAQRAYAELVHETPGFVEYFKASTPVSEVGALN
                     IGSRPTSRKPTQSIADLRAIPWVLAWNQSRVMLPGWYGTGSAFQQWIAAGPESEGERV
                     AILHDLYQRWPFFRSVLSNMAEALAKSDLGLAARYAELVPDESLRRRVFDKISDEHQR
                     TIAMHKLITGQDNLLADNPALARSVFNRFPYLEPLNHLQVELLRRYRSGDDDELLQRG
                     ILLTMNGLASALRNSG"
     gene            complement(15456..16436)
                     /locus_tag="VFC01_28765"
     CDS             complement(15456..16436)
                     /locus_tag="VFC01_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HZN83646.1"
                     /translation="MMSRLRVDPYILALLLTVAVASLFPARGAVATGFGHATTIAIGL
                     LFFLYGARLSTREAVDGLKHWRLHGLVFLATFVLFPLLGLLCLLLVPWALPPALYAGV
                     MFLCCLPSTVQSSIAFTSIARGNVAAAICSASFSNLIGIVLTPLLVTVFLFTGTGGVS
                     LGAVRDIMVQLLVPFVAGQLLRRWIGGFITRHKKVLGYVDRGSVLLVVYSAFSAGVVG
                     GIWGRLSVVSLLVLFAVNAALLAVVMVGTTFAARRLRFSTEDAIAIVFCGSKKSLASG
                     LPMGTVLFPAATVGLAVLPLMLFHQLQLMVCAWLARRYGAREDAVALASA"
     gene            complement(16433..17287)
                     /locus_tag="VFC01_28770"
     CDS             complement(16433..17287)
                     /locus_tag="VFC01_28770"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HZN83647.1"
                     /translation="MTYGSALRRDVNGNSITPLIGVFDMFSASLSAQHYGGMFVSGFG
                     FAASYYGLPDIGFIAWPDMVNFVQRLRLAFPQHHLLVDIDDGYVDPEVACHVVENLER
                     IGASGVILEDQKRPRRCGHVDGKQILPLDEYLEKLNLVLETRKDLVVVARTDATEESE
                     ILRRAEALAATDADVLLVDGVRSVDWITKVRGVIGDKPLLFNQIAGGKSPRLSLTELE
                     DLGVNVAIYSTPCLFAAHTAIDNALLELRANDGRLPEVKEGDVGVAASIDLLERNISR
                     HHPKIDRV"
     gene            complement(18537..19706)
                     /locus_tag="VFC01_28775"
                     /pseudo
     CDS             complement(18537..19706)
                     /locus_tag="VFC01_28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3532 a         6276 c         6345 g         3639 t
ORIGIN      
        1 aagttcatgc ccaccgccct cgccgcggcg gcgatcggac tcggcgcatt gatgaccgtc
       61 ggcgcaccct ccgcgcatgc aagcgaaaag tccgactgcg aaggcaaggg cggcacgtac
      121 acggagacgc agatctacaa ccaaacgacc ggcaagtccg gcacgaacta ccgctgctgc
      181 gtcaaggaca cagcgacgaa taccaccacc tgcacgagca cgacggtgac caagaacgtt
      241 cggattccgg tgacagttcg gccgggggtg atcgacaccg ccgaaaccgt cgagccggct
      301 cagccgattc aacgcgttcc cgtcgacgta ttgaacaccg acgcgcagat ccagcagacc
      361 ccgtaacggg tcattccaga aaccaaatca agcgaaggat tcgacaatgt ctgagacgac
      421 gcgttacaac ctcgagaacg atgccgttga ttggcgcgac ctggcggacc agatctttcc
      481 agcggacgcg ctgcggacgg cgccaacggt ccatccattg gcagcggtct aggtcgcctt
      541 cagacgattg ttgccaacgc taaatttgcg tttgctacca atataattta cgcggattcc
      601 gcagcgaaat cggcgtgccg cctccagaat taggatagct ggcgccgccc gggctaacag
      661 ctattgtgag caatcgacgc gtcgccggcg ttgggggcgt gatgagcgta gtcgaggcaa
      721 tttcatttgg gcactaccgc ctgaccgacc tgctgggccg cggcggtatg ggcgatgtgt
      781 ggcgagccca cgacacgcgg actgaccgaa tcgttgcggt caaactgttg ccgcggaaat
      841 tggcccacga cgacatcttc aagcagcggt tccggcgtga agcccgtgcg gcagcgcagc
      901 tcaatgagcc gcacgtggta ccgattcacg gctacgggga gattgacggc cagctgtacg
      961 tggatatgcg gctgatcgaa ggccgcgatt tgcacgatct tctgaccacc gggccgttga
     1021 agcctgctcg ggccgtgatg atcattgatc aagttgcgaa ggcgttgcat gccgcgcaca
     1081 acgtgggctt ggtgcaccgt gatgtcaagc cgtccaacat cttggtcgcc gactacgact
     1141 tcgcgtattt gattgatttt ggaatcgcgc gcggcgctgg tgaagccacg ctgacctcga
     1201 tcggcacctg ggtcggcacg ccctattaca tggctccgga gcgatttcgc gacgggcctg
     1261 ttgatgcccg ggccgatatc tacgccttgg cgtgcgtact gcacgagtgc ttgaccggca
     1321 ctacgccatt tccggggtcg agcatggaaa gccaaatgac cgcgcatctg acgatgccgc
     1381 cgccaagacc gtcggtcgtc gtgcgcggag taccggcaac gttcgacgcc gtgatcgcta
     1441 aaggcatggc caaagatccc gatcagcggt accggaccgt tctggacctt gccgccgcag
     1501 cccgttcggc atcgtccact ccgattccaa cgcttccgcc accgcccccc tccgaggagc
     1561 ccaccgtccg agaggccact ctctatgcgg gcgatgagga cgcgaccgtc cgcttcggcg
     1621 agcccgggaa accccggccg ccgcgaaatc ggcggcgcct ggccatgatc ggcgcagcac
     1681 cgaccgtggt cgttctcgcg ctcgtcgccg ctgtttccct gtgggtcggc cgcgacaagc
     1741 cgggagtgag cggcccggaa atcttcctgg aaccggcgat ggcgctcggc cagaacccgt
     1801 tcaccccgtt ggcttcgaga ccgcagccgc cggcaacatt tccggggagt gatccggtcg
     1861 gtgcgccggt gtcaggcggc actgatccgg acagggggcc ggtgtccggc ggccaggtac
     1921 gagcggtacg cggcgacgtt ccgggcctgt acggcggcac ccgcaatctg agcccctgcg
     1981 acaccggcct gatgacttct tcgctcggac agaaccccga ccgaggacaa gcgtgggcgg
     2041 ccacattcaa catcaccgct gctcaaattc cggcctttct ttccggactc acgccagttc
     2101 tgctgcgcac cgacacacgg gtcaccagtc acggcttcgt ggatggacag gcgtcgccat
     2161 accaggccgt attggaagcc ggaaccgcgg tattggtcaa caacttcggt gtgccggtgg
     2221 tccgctgcgg cggcggtaac ccacttctcg agccggttgc aatgacctcg acgaggtatt
     2281 ccggtgaacg gtggaagtcg ttcacgatag gggcggtgac cgtcatccgg cccagcgaca
     2341 acccagtcga cgctttcatc ctttacgact cgacctccgg cgactcgttc gcccgtcctc
     2401 tcggcggcaa tggatccaac gacttccccg atccacgcgt cccgacaaca tcaacgcttc
     2461 ctcgaccacc tctcgaaacg cagacccaag cgccgccgcc tccgccgatg acccgcgtga
     2521 ccgacttccc gacgtatgcg ccgccgtcga tcacttatgc gccacccatt ccgacgcaac
     2581 tgccgcgagc aacgaccgcg actacggctc cacgcgagac cagctccgcg ccacggacga
     2641 gctactacaa gccgccgacg accaccctcc cgaggcgcga ccgatcaggg gtgggcggat
     2701 cgacaggtac gccgtcgcaa aagccacctc catcaggcag caaggcgccg gagcctacgg
     2761 cgccggcgcc gaagccgccg ccgaagccgc tctgacaaca cctgaaaccg gttgcggtac
     2821 aacggtttta gtttgcaggt gcgcttcttc caccgggcga gccatacaga tacgggtagt
     2881 gcacgcctga atcgttggcg ggacgttgat caggcggcgg tggtgctgtt ctccacgctg
     2941 ccgggaggcg gcagccggtc gaatagccgt agaacagcac ggtgttcgga ctgttgatca
     3001 actcgacggt cgcgccatcg tcgcggatct gcgagaacgt gccgggcgcg ggagcggtgt
     3061 tcggtggcac gttcggcgtg aacttcgcgg cggcgaagat cggcgtgagc gcgtcggtga
     3121 tctgcgtcca ctgctcgtcg gtcggagcag gccgcgcgta gatcttctcg atcgtgtacg
     3181 tgtcaccgat gttgtgcgta aacggatcga cacagccgcg atctggcttc ggagtcgacg
     3241 gtcccaccac ggtcgccgca gagtatttcg cgataacgga acggatctgg gcgtcgaggt
     3301 cgatgagctg ttgctcggcg acctcaaggt ctggccggtc gttgaggatc ttctgcaacc
     3361 ggtccagttc ggagttatcg ggcgcggcgg ccgggttgaa cgtggagggt ttcacgcacg
     3421 aaggcaatgc cacgctgagc agtacggcga tcagcacgcc gaccatccga tgtcggcgcc
     3481 tcatgggtga tagttcgggt taggaaccgg catgccgtgg ggcccgagcg gcaaagttgg
     3541 cggcggcagg ttctgtggcg gtgcgatggt ctcgccgggt aaccctgcca gtatcgctgc
     3601 catgttgtag ccactcatgc gtagttctcc gttgctacct aaccgtgggt attccgaatg
     3661 tccgtacgcg cgttcgtgcc attgcccatc accatacggt gggggcaacg cttgtccgga
     3721 gttcgacgat agttcggtaa agcccggtac ctcactcagc ggcggaccga agttgtgggt
     3781 cccggcaatc gtggtgggca ccccatcggt gatgccgttc atgtagaagg catgtcccgg
     3841 tgcgacaccg agttgcgagg cgttgctcag ctctgcgcct ggtgaaccgt agagcaccac
     3901 gttgtcgact ggagcgcctt gctgcagcgc caaactcgtc gtcaacgagc cgtacgagtg
     3961 cccgaatgcg gtgatctcct gatcgggcac attcgaagtc gcgccgagtc cttggtagaa
     4021 gttattcagc ggcccggcac ccgctttggc gagcgcgtcg tccgtgacat cgaaattcat
     4081 gccgggcgct tcgtagccca gccaggcgat cgtcgccacg gattcgggat tcgccactcc
     4141 tgccaactgt cgcagttcga ccgcttggtc gtactgagtg atgatctcgc cggtcatccc
     4201 gtcaacactg cctctgacgt tggtggtcat gcctcccacc gtgactccca cctgcctggc
     4261 gttgtcgacg tcgcctttgg ccacggcggc cagcaccgtt ttcgggttac tagtggtatc
     4321 cagcagtagc aacccgaccc cgggatggtc cttcagcgtc ttatccagtt gctgtagatc
     4381 ggcgagcttg tcgcgtgcgc cctgccatgc cgccagctgc gcgggattcg gctttccatt
     4441 cgggtgggcc cgcatgtagt cgacgagcgc ctgggctttc gcgatctctt ccggcagtcg
     4501 tgccgcgttg acttgttccc gtaccgcagc ggggatcccg tcgaggttgc cgatctcggc
     4561 ggcatgcgtg cgcgtaagtt cttggcgctg ctcgttggaa agcgagtccc accacttctt
     4621 gacgtcagcc ggctccgtac ctggctgcgg cagaggcgga ccagcaggct tctcgtctgg
     4681 aattggcgtg gcgccggtgg ccatgttgat cgcctgcgca agctcttcgt cgacccgcac
     4741 agcctcggcg aggatcgcag tcagccgcgc ctgtagctcc tcccgtttgc cttccgccgc
     4801 aatgagatcc accatcgaac cccgcaacgc agggcctggc atcaccgcgt tgctcgcccg
     4861 gtcgatctcg aaaccagcac cggcggcgtc ggcttcgagc tgtgccagat cggacttgac
     4921 ctgctccatg tcgtcggccg cgcgttgcgc cgccttagcg acggcaagcg cctcattccc
     4981 gtgcgcatcg agatccttac ggagttgctc gttcgccgtc ttcgcggcct cggctgcgtc
     5041 gccgccccat gatccgaacg cgggcagcgt cgcaatcccg tcggcggctt caaaggccgc
     5101 ttctgctctg ttgcgggtcg cgtggaagac ctcgcggaca tcgccggggt cccaccgatc
     5161 gatgtcggca attgagaccg tcacggcgac gaaccgttag agcccgagat ccaaagtggt
     5221 ggcctgatcg ccggctgcct cgatggcggc ggccttgtcc tcatccgtgc ccatgtagcg
     5281 tgctgcggcg ccccgcagcg cctcgccgtg ccccaccaaa ttgccgaaaa gcaccgtcga
     5341 gtcggcttgc cacttggcaa ccgccgcgct cagcgccgct gcagatccca ttggcacgcc
     5401 gacctgggcc ccctcgatac gaccgttggc agtcgcatgc cgtagatgca gatcgtcggc
     5461 atgcacgtcg acagtcgccg cggacacatg caggtcctct gcagcgacgc gaatccagcc
     5521 cgacacggag ccctcctcat gagcccagca aacgcctaac ctcaccagaa tactggcgcc
     5581 gcggaagggc aaggtgcacc aattttgcgg ctgtacgaat acgcgaggtg cgcacttggc
     5641 gcaccccgcg tacacgtatt gagttgttaa cggctgggaa ggcctggcca gccttcccgc
     5701 cccccgctac gcgccgctct tcgcggcctc tgccttcgcc agatccggga tggtttgcgt
     5761 atcaccgtcg agggtcgaag tgatgtaccc cgtcaccgtt tgggcgaacg ggctgaccac
     5821 agcgttggct tccaacttgc ccctgtttgc tccggtggcg tcggtgacat cgatgtcggc
     5881 gaggtacgtc acgttgcttt ggctcggtcc gtagatagcc ttttcggtgc cggtgaggat
     5941 caatccgtcc tcgttgacgt atttggtgta gaccaccgtg cgggtcgttt ggcgtgtcgt
     6001 cgggtcaaag gactcgctga ccaccgcggt gcctccgccc acgccgctgt aggttccggt
     6061 cggcggcagc ggtgtcgtct tggcgacgta ggtggtcagg tcctggaacg ccgacgggtc
     6121 gaacactgtc gtcgtatccc cgaccggccc gacgctggtc gtgtacttca ggttggcgat
     6181 cacgattcgt gattccgtcg gcgccacccc gtgctcgtcg ccgttaccgg actcccagaa
     6241 ggtgaccgcg gtgccgtcgg cattccagct gggcatgctt cgggaagccc atccgtcacc
     6301 ttcgtccacc tcgggggtgg tggggtcgtc ctgaacgaag agcggaatac cgttctcgcg
     6361 gtcgcggtcg tcctcgaggg caaccgccca attctggttc gagacgttga ccacttgatc
     6421 atccgcatac tgcgcataga tcgccgtgac gtagaccggc aagaagttct gccgcacgat
     6481 cctggtcatc ggcgtgagcg cgtcgaatcc gcgcgtgctt ccgatcgcga tccactgcat
     6541 gttgggcgag aagtccatgt cctcgtcata gtcgaggttg ccgctcaccc gagtgccgcg
     6601 gaaggggctg ccagggtaca agttgtcccc tgtttggccc gacaggtcga ccatgatgtc
     6661 gtcgatattg ccggcatcga ttgccccgcc ctgaatgacc acgcccttgc cgtcgggggt
     6721 ccattgcttg ccctcaccgg tgggccagac cacccgcgcg ttggtgacgt cgtagtgatc
     6781 accgttttgc gtcaactcgc ccacgatcgg aagggcttgc aggccaccgg tttggccgaa
     6841 gacgatgcga gtgaacagca catgcgtgcc atcaggtgac ggcctcattt cccgctcccg
     6901 gttcacaatc ccgcctggga aaggcaggcc gggcagggtg gcgccgccgc catcgggcgt
     6961 gatgacgttt acgatccgcg ccggtgagcc tatgccgttg tagccggccg gctcaaagat
     7021 cgcataacga ggttccggat tatccaggag caccacgact cgcccggagc cgtcgtagaa
     7081 gggaaccggc tttcgcaggt tgaccggcaa cgacggatct gacgtcgcaa ttgttggacc
     7141 ggcgacgcca caggtgacgc attcagtgtg cgtgccatcg atactgatct tgtagatctc
     7201 ggaccgcccg ccggcagtcg gtgtgccggc gaagtagatg gactttccgt cttccgaata
     7261 gcgcggattg gccgggttct tgacgccgtc gggcatgttc aggatcaccc gctcgacggt
     7321 gggaggctgc accttgaatt cgatggtctc ctcgtcgcta tgcggtttga acagactcag
     7381 caagttgaac ttgccgtcgg tgaccgacac cgtgaacgaa acacgctgct ccgagtcgta
     7441 gtcgatctca tcgggcgtgt aggcgaactt gcccgtcgcc tggtcgatcg tcagggtgcc
     7501 gtgcgccgga gcttttgtga ctgtgtacgt caaggcgtcg ttctccgggt ccttcgcgcc
     7561 gatgtagccg atcacggtct gcccggtctg ggtcgtagtg ccggggttgt acgtgatcgt
     7621 cggcgcctga ttgaagaagt tgcgtcgaac ccagcccagc accgcccata tcaccggggt
     7681 ggcgggatct ggtgtgttgg gcgccggcgc caggaacggg ttcagcagat tggtcacgac
     7741 ggcgttgagg aatcccacga cgccggtggc gggagtggtg actagggccg ccgcggcggt
     7801 gggtgcggca gcagcggcga tcgactgggt cgtcagcgtg ctagagctgt tgaccgtcaa
     7861 cgcttgtgcg gtcggcaccg cgctggactt ggtggtcgtc gcggacggtt gcgcgacggt
     7921 cgcgacgacc tttgcagctc cgatggaggt cgtcggcgac gacggcgccg tcgcgtcctt
     7981 gaccgtggcg ggatgtgtcg agctcgatcc ggtcttggcg gaagtggtct gctccggtgc
     8041 tggggtcgcg ggcggcgtgc tcggctgaac gggctcaacg gtctcgccgg aggtggctgg
     8101 ttcggtcgag tttggttggg tctcaacgac gctggttgac gtcggagcct tcgatgatgt
     8161 cgattcggtg gtgctggtgt gcgctccgcc gctgctgctg atggtgacac cgggcgcaac
     8221 ctcggtggac gaagacgtcg cgctactggt tgaggtcgat ccgccgcttg tcggcgtcga
     8281 cccggtaccc gtcgtcgcgg tggacgaggt ggcagttccc gtggtcgacg tggccgcggg
     8341 tccagtgccc gactcaccgg acgacgtcga gctcgccgaa ctcgttgagg acgagtccga
     8401 ggacgtgccg gtcgtatcgg ccgcggccaa tccatagccc gtcgccaccg cggtgacaag
     8461 ccacgctgcc actgccatgc cgccgtatcc gccgagcttt gcggcactgt gggctgctcg
     8521 tcgtgacgag gcggacgcga acagggggtt gacgggtgga taggtagcgg tcatgggctc
     8581 gactccggtg gcgtagcgac caatcttgat cgatcgatca aatgataagg tggcgcatgt
     8641 ttatcacgat ccgaaacttt cggtcaaccc cctcgcgact ggggtcttga atggcaacgg
     8701 cgcggaaggc gaaggcagac gacggcggaa cccgacgccg tctgctcgat gcgaccgccc
     8761 agatcatgca cgacgagggg tatgccgctg ccacatcacg ccgagtggcc gcaaaggccg
     8821 gcatcagaca gcctctcgtc cactactact tcccgacgat ggacgacttg tttctcgcag
     8881 tgctgcgcgc gggcgcggac gtcgcactag atcagatgcg cgcagcgctc accgacaaca
     8941 acccgctgcg cgccctttgg gtcctcaata gcgaccctcg gcgaagcgtc ctcaataccg
     9001 agtttatggc acttgccaac caccgcaagg caattggggt ggagcttagg gcatacgccg
     9061 agcgtgtgcg cgatatggag accgcagccg tcacgctggt gctgcgcgcc catggcgtcg
     9121 acttgaacgc ctacccgccg gtcgtgatct cgatgctgat cgcccaaatc gcacgcagcc
     9181 tttgcaacga ggatgccgtc ggagtcaccc tcggccacga cgagatgcgc cgcttcgtcg
     9241 agcagcagat tgacgcactg gctgagccat ccaccgtctc gggcagctga tctcgcctag
     9301 cccgcgtctg ctgccaacag gcccattcgt aaacccagat gcgccagcga cacatggtgt
     9361 tgacggcgcg cgcgaccaaa tgtccaaggt ttctgatcga tcgatcaata tgaagactgc
     9421 gttgctcaca gcagccgcgg aaccgcgacg tcgccggcga ggccgaaaac aaggtgctcg
     9481 acgaggtccc agcccatgta gaacgtcggc gcgaaatctt ccggctcctc gccccacgat
     9541 tgctcgaacc cgatcaggcg ccggcgcgga ttatcgggcc acaggatcgc gttggcgagg
     9601 tactcatgcc cgccggcgtc ctcgaggggt gggtcgcacg ccgacgttat gacgccgcgc
     9661 tcgccggtgg tcatcatgtc ttcgaaccaa tacgagccga cgagcacggg ttggtgttgc
     9721 agggtcgcca gcagcgagtc catgccgcca ctgataatcc agtcgtatcc ggtgatgacg
     9781 ccgagctcgc gccaaacagg tcttgattcc atgttgcggc gagcgtgtcg gtggagccta
     9841 tgccgcctct tcatgaagct ctggactcgt caaaattcgc agaaactctt ctgtttggtc
     9901 atgtgaagtc gtcgatttgc actcagccaa ggcttcgctg acggccgcga ctagcaacgg
     9961 catgacttcg gcttgaatgc cgtctttgtc agctggggcc atgggccgcg gtttttcacc
    10021 tgacttcaca aagttgaata tctgcttgaa cgcctccgac cgctcggtgg ccgcgggaga
    10081 atttatcgca gtctctgcag ccgcgaacgc cgcgaccgtc gctgccagct tgtcagggtg
    10141 ctccaagagt tccgcgaccc gggaatcgcg aggacagcct ttcgtaccct gctccccatg
    10201 caaacgcagg atcgcctcca ggatgttcat caccccttcc atgtacacga cttcgaatcg
    10261 ctggtgcttg aacagcctga ttgcgttatc tccttgcaag tccgcacttt cgggcagaac
    10321 gggaaccccg aaccgccgat gcatcaggac aaccgtcctg actgcactct tcacttggct
    10381 gggactgaat gcgtacgtgg tggcgaacct ctttatggca ggctggaaat aatcgcccgg
    10441 ccgcagcgat gccagtgcgg cacggccgac gtagggaccc accgcgatga gcgtgttggg
    10501 caccttgatt tcagcactga catatgcacg aaagcattca ggactgatat agccatcttc
    10561 attggcttcc gacattgcca cttccttgat tagagtccct gcgcggtgtg atccgatgct
    10621 ctcaagtcag tgtgcgcaac ggcgtcgctt ttgtacgccc gtttgccgga gatccaggtt
    10681 tcatcgacgt tgcggtcgtc gccgaccatc atgacgccga aaagcagctg tgccgcctga
    10741 tccacagtct ctggggcacc ctcgacgatc agcgattggc gccaccgcat ggccgcctgc
    10801 ccggcgttcc agtcgatcgc gacgaagtcg gcctccttac ccttggcgaa attccccagg
    10861 aggtcgtcga ggtaaagggc gcgagcgccg cccagcgtgg ccaggtagta ggcccggtag
    10921 ggcgacagga tgttgcgctc ggcctccgct gggtcctggt cacgcgggtc gaccgagccg
    10981 tcgagcatgg tgttgttgca cgtgccgacc ttgtaggcct cctccaggac gcggatgatg
    11041 ctgagggcgt tgccaccgcc catgtcggag cccagcgcaa cgcgcaccgg gtgctcgggg
    11101 tcgagggccc ggccgagagg gaacaggccg ctgcccagaa acaggttgga tagcggacaa
    11161 aaggcgatgg ttgctcccgc cgcggagaag cggcggaact cgtcgcggga cagccagaca
    11221 ccgtgcccgg caacgaattt ggggcccaag aggccgtggc tggcatgcac cgccgtgtag
    11281 tcctgacaat ccggaaattc cgaccgcacc atccgcacct ccgaggggtt ctcggagatg
    11341 tgggtgttga tccagcagtc ctggtgctct tgcttcagct gccggcagcg ctccaacatc
    11401 tcggagctgc agacccccgg gaagcggggc gtgatggcgt aaaggttccg gcccgtgcgg
    11461 tggtagcgct cgatgagccg cttggattcg cggtagaagt cgtcggctgt gatcaggaaa
    11521 tcgtccggtc cgtactggtc gatccctgcc aggccggcga tgacgcgcat gccgcgccgg
    11581 gtcgcctcgt caaagaactc ctcggtggac accgggaagc tggtggtgaa ggccatggcc
    11641 gtggtcgtgc cgccggccag cagtgcgtcg aaaaagtacc cggcggcggt gcgcgcgtag
    11701 ctgcggtcct tgtatttgag ttcctcgggg aagatccagg tctgtatcca gtcgagcagt
    11761 tgaccgccga agacgcccag ggcgcggagc tgagggaagt ggatgtggcc gtcgatgaac
    11821 ccgggaagga tcaggcgatc ttggaggtgc gtcaccggca ggtcggcgtg gcgccgggag
    11881 aggtcggcga agtgaccata gtcctcgatg atgccgtcct tgacgaccag gaggccgtcg
    11941 ccgacaaagc gcgcggcccg gtcctcatgg ccggggtgct tccacgggtc gtccacgaaa
    12001 tcgaagaagc tgccgcggat ggcgcaactg gccggcatca aatgtttcct cggcatccgg
    12061 tgcgaaacgg caatgcgaaa ggctcagact acgcgtcccc tggcgccatt ccgtggaggc
    12121 gaggacgtga ggcgtggcgg cggctagccg ctgttacccg ctatttcgga gcgcgctcgc
    12181 cagcccgttc atcgtcagca agatgccacg ctgtaataat tcgtcgtcat cgcccgagcg
    12241 gtagcggcgg agtaactcca cctgaaggtg gttcagcggc tccaggtacg ggaagcggtt
    12301 gaacaccgag cgcgccaggg ccgggttgtc ggccagtaag ttgtcttgac ccgtgatgag
    12361 tttgtgcatg gcgatagttc gctggtgctc atctgaaatc ttgtcaaaca ccctgcgccg
    12421 caatgattcg tccggcacca gctcggcata gcgggccgcc aggcccaggt cgctcttcgc
    12481 cagtgcctcc gccatgttcg acagcacgct gcggaagaac ggccaccgct gatacaggtc
    12541 gtgcagtatt gccactcgct cgccttcgct ttcgggcccc gccgcgatcc actgctggaa
    12601 cgccgatccg gtgccgtacc aacccgggag catgaccctc gactgattcc acgccagcac
    12661 ccacggaatg gcgcgcagat ctgcgatcga ctgagtgggc ttgcgtgacg tcggccggct
    12721 gccgatgttc agcgccccga cttcgctgac gggtgtggaa gccttgaagt actcaacgaa
    12781 acccggtgtc tcgtggacta attcggcgta agcacgctgc gccagggtgg ccacctcgtt
    12841 aagcactcca taagctggtt ccgcaccgtt acccaggccc tcgacatcca gcagcgtcga
    12901 ctccagggtg gctgccacca agctctccag attgcgtcgc gccagttggg gttcggcgta
    12961 tttggcggcg atgacctcac cctgttcggt gagacgcagc gagccgttca ccgctcccgg
    13021 tggttgcgcc aggatggctt ggtaactggg gccgccaccg cggccgacgc tgccaccgcg
    13081 accgtggaag agccgcaacc ggattcccgt tttgcgggcc acttcgacca aggtgagttc
    13141 ggcccggtag acggcccagt tggcggccag atacccgccg tctttgttgg agtcggagta
    13201 gccgagcatc acttcctggc atccgccccg agcgtctacg agggatcgat agagcggaag
    13261 ttctagcatc gcgtgcaaaa tcaaggcccc gttgtgcagg tcgtcgatcg tctcgaacag
    13321 cggtgagatt cctaccgggc aatagggttc cgcccctgag gcatccaaca ggccaacctc
    13381 tttcagcagg atcgcggcct ccaagacgtc cgacaccgac tggcacatcg agatgatgta
    13441 gttcggcact gcggccgcac cgtagacctc gacggcgtgg gcggcggcgg cgacgacgtc
    13501 cagctcgccg cgggccaact gtgacaattg cgcacggtcg ctgaccagct gccgacgggt
    13561 gctgagttcg ctcgtcaata gctcgacccg cttatcttcg ggtagcgacc catagtccgg
    13621 gtgcaccccg gccgacgcca gcagttcgcc gaccacctct tcgtgcacgt cggagttttg
    13681 ccgcatatcg aggccgcaca ggtgaaaccc gaagacgtgc acgctttctc gcaagtgtgc
    13741 cagccgatcg tcggccaata acgcggcgcc gtgcgtgcgc agcgaagcgt cgatggcgtc
    13801 gagatcggtc cgcagttcgg ccggtgtgcc atatgggggc aagcccaggt caagaccgtc
    13861 ctgcggctga tgatccagga tctcggccgc ggttgcggta agccggccgc ggattgtgcg
    13921 caaggcccgc cggtacggct cgtcgaggtc gacggtatcg gtgcaaacgt cggccagcgc
    13981 ggccacttcg ggggtgacgg tgatcagccg cgccgacatc gacagctccc gctcgaggtc
    14041 cgttagttcg gcgaggtagc gcgccagcgc ggtgaatgct gcgctgccgg ttgccagccg
    14101 gaccacgtcg gcggtgacgt tcggatttcc gtcgcgatcg ccaccgatcc acgatccggg
    14161 ccgcagaatg ggctcggcaa gcagatccgc gtctggccag cgggcacgta acgcttcacg
    14221 aacgtcggcg ttgacctgcg ggagcacctc gaacaacgcg gcagcgtaat accgcagccc
    14281 aaccgcgatc tcgtcggtga tcttcagccg ggacaaccgg atcagcgcgg tctgccacag
    14341 cgtgaggacg tgacggcgca actccaattc gatgctgcgg ccgtcgttgg tctcggtacg
    14401 tccttcggca tgctgccgca tcagctcggt gatccggtgg tgggtgacaa aaacggtgcg
    14461 ccgccgggtc tcggtgggat gggcggtgat caccggggac accagcgcac cctgcaaggc
    14521 gtccgcgacg ctggacgaat ccagctccgc cagatcgagt ttcgcgtaag cggcggccag
    14581 gctgccgtcc tggggcggtt cgccggcgac gtgaatgccg cggcggcggt cccggtggat
    14641 gtcttcggcg acgttggcga acagggcgaa atggctgaac gcctggatga tcgggatcgc
    14701 caggtggata tcgatgccgt cgaatatgcg cgacatctcc gcccggtcga tctccgagcg
    14761 tcgcacccgg aaggattcca cccgggcgcg ttcgacgagg tcgaacacct cgtcaccgtt
    14821 ctgctcgcgc acggtgtcgc cgaggatggt gcccagcagc ctgatgtccg cacgcatcgg
    14881 ctcgctcgcc tcgcgaccga cccgggtccg ctggacggcg cgggccgcga gcggtgcgaa
    14941 gaacatgtcg aagacgccga ggagtggcgt gttgctgtca ccgtgctcct cgcggcgcag
    15001 cgcgctgccg taagtcatga ttgaacccct tcagatgagc gaggacgtgt taactggcac
    15061 atggtggtcg gttgccattc gttgaagacg ccgggtatta ccattcttct ttcttcctgt
    15121 ctgtgcgatg tcgaggtttt ttgcgcggct attcgcgatg acttttcgcg atagctgagt
    15181 tggttagcgc cgcgatcgtg cgacgtattt ggatttggag tcaccgaatt tacggcgctg
    15241 ctacggatgt gtcgctcgtg taagcgatag tgggaatagg caacgatttc acggttttgc
    15301 gcattgtgct gactcggcat cggcgcgaca gagtgcggtc ggccagaact catctcttca
    15361 caggtgagct ggcgaactgc atccggcggg cccggtagtc gcgtgcgacc gggcgggcgg
    15421 ccctgccagg acccgtggac cgcccgcccg gccgctcagg cgctggccag cgcgacggca
    15481 tcctcgcggg cgccgtaccg tcgcgcgagc caggcgcaca ccatcagttg cagctggtgg
    15541 aacaacatca gcggcaatac ggccaagccc accgtggccg cggggaacag cacagtcccc
    15601 atcggcaggc cgctggccaa cgacttcttc gaaccgcaga acacgatggc gatcgcgtct
    15661 tcggtggaga accgcagcct tcgagccgcg aaggtggtcc ccaccatcac caccgcaagc
    15721 aaggcggcgt taacggcgaa cagcaccagc agcgacacca cggacagccg tccccagatg
    15781 ccgccgacca cgcccgcgct gaacgcggag tacaccacga gcaggaccga gccgcggtcg
    15841 acgtagccga gcaccttctt gtgccgggtg ataaacccgc cgatccagcg ccgcagcagc
    15901 tgaccggcca cgaacggcac cagcagctgc accatgatgt cccgcaccgc gccgagcgag
    15961 acgccgccgg tgccggtgaa caggaacact gttaccagca acggcgtgag cacgatgccg
    16021 atcaggttgg agaacgaggc gctgcagatc gccgcggcca cgttgccgcg ggcaatcgag
    16081 gtgaacgcga tcgaggactg caccgtcgac ggcaggcagc acaggaacat caccccggcg
    16141 tacagcgccg gggggagcgc ccacggcacc aacagtaggc acagcaagcc cagcaacggg
    16201 aacaggacga acgtcgccaa gaacaccagc ccgtgcagcc gccagtgctt gaggccgtcc
    16261 accgcctcac gggtggaaag ccttgccccg taaaggaaga acagcagccc gatggcaatg
    16321 gtcgttgcgt ggccgaagcc ggtggccacc gcccctctcg ccgggaacag cgaggcgacg
    16381 gccaccgtca acagcagcgc caagatgtag ggatcaaccc gcaaccggga catcagacgc
    16441 ggtcgatctt ggggtggtgc cgggagatgt tgcgctccaa caggtcgatg gaggcggcga
    16501 cgccgacgtc gccctccttg acctccggca gccggccgtc gttggcgcgc aactccagca
    16561 gcgcgttgtc gatcgcggtg tgcgcggcga acaggcacgg cgtggagtag atcgccacgt
    16621 tgacgccgag gtcctccagc tcggtcagcg acagccgcgg cgacttgcct ccggcgatct
    16681 ggttgaacag cagcggcttg tcgccgatca cgccgcgcac cttggtgatc cagtcgacgc
    16741 tgcgcacgcc gtccaccagc agcacatcgg cgtccgtggc ggcgagcgcc tcggcgcggc
    16801 gcaggatctc cgactcctcc gtcgcgtcgg tgcgggcaac cacgacgagg tccttgcgag
    16861 tctccagcac caggttcagc ttctccaggt actcgtcgag cggcaggatc tgcttgccgt
    16921 cgacgtggcc gcagcgacgc ggacgcttct ggtcctcgag gatcacgccg gacgcgccga
    16981 tgcgctcgag gttctccacc acgtggcagg ccacctcggg gtcgacatag ccgtcatcga
    17041 tgtccaccag caggtgatgc tgcgggaacg ccaggcgcag ccgctgcacg aagttgacca
    17101 tgtccggcca cgcgatgaaa ccgatgtcgg gcagcccgta gtaggacgcc gcgaagccga
    17161 atccggagac gaacatgccg ccgtagtgct gggccgagag cgatgcggaa aacatgtcga
    17221 agacgccgat gagtggcgtg atgctgttac cgttcacgtc gcggcgtagc gcgctgccgt
    17281 aagtcatgac tgaaccccct tgaaggggcg aagacgcggc aattggaccg cgggccggtt
    17341 gccgtttgtt gagggtgcca ggtactacca ttttctttct ccttgtgtgt gacgtcgacg
    17401 ttttttgcgc ggctattcgc gaatgagttt cgcgatagct tgagttggtt ttgcgttgag
    17461 atcgtgcgac gtattccgat ttggagacac cgaatttagg gcgatgctac gggtgtgtcg
    17521 gtcttgtaag cgatagccgg agtaagcaac gatttcacgg ttttgcgcat tgtgctcaga
    17581 tggtgggttc gccgaccctc acggcatttc gtaggtttgc tggcggttgt gcacctgtga
    17641 ccacaatgcg caaaagctga aattcgcgca atgcgcgagt tgtgcggaca agcgagccac
    17701 ggcgattgcg gacccctaac ttcggttgtg acacgtcaac caactggtcg acatgtcacc
    17761 agaaccgggt aagagccaaa tcgctactca cacaggagaa ttcaaatgaa cgcaactgat
    17821 ccaatccgcg cggagctgcg cgctaatgct gccgcgtatg gcactactga ggactactgg
    17881 gctagctacc tcggcattgc cgacattgaa ggaccacgtc agtaacgagg agtaggcaga
    17941 tgcacgagga gatgatggtc ggcttccgtc gacgtcgcac ccagagcagg agcggatggg
    18001 ggacgccgag gccatccctt gtcgcatcgg atgtcgtctc gtcccgccta gttgtggggc
    18061 ccgtcgctgc catcatcggc ggtgccttgg gattaacggc cgacgctaat gcggcgaacc
    18121 ccgaccagct gaacctcaat ccgcggccat gtcgaaggca ccacctcgtc atcaccgccg
    18181 tcgtgctcga agggcgtagc aaaagcgagg tggcccacac ccgaaaacag gccgtagatg
    18241 ccaatgatgt ctcgggacac ctgtcaacga tgtcccgaga catcacactc caaggtgcat
    18301 acccaagtgg ccgcctccga gcgcgcagcg ttgcattgac gcaacctctt tgccgcccaa
    18361 ccaccttgca atgaccgccg gagcggttca tccaggaaca ccgtggcacg gcactcatcc
    18421 acgttcattg gcctatccca gatggtgact aataagccca cagcgtgtcg cgcaccggcc
    18481 gcacaagttc cttgcgcgcg caggcagccc gtagcgaagg gacgtactaa ctatccctaa
    18541 gggttgcctc caaagggggg ccgcgccgcg tcgacaagcc ccgacttcac caatgcactg
    18601 tcgatgaatc gctgcaccgg ccgtgagcgg aaaaacccgg tgtggcctcc cggataccaa
    18661 cagatttcgg gtctgcccca gtgctcccag agtcgggtcg cctcgtcgcg cgggtgcacc
    18721 agcctgtcag cgatgccagc acatatgaag cggccctgca tcggcacgcg cggagtgagc
    18781 ccaagcggcg aaatcatgcg gctgatgggc ttggccaacg tgagcatctg gcgaaggtca
    18841 aggtagtcgc tcacgccggc atggcgagcg actagctcga ccagatcggc gagcgggacg
    18901 ctgacgatcg cacaagcgag gtcgtcctcg aggctggcga tcagcgaagt gagatagccc
    18961 ccgagcgaga tgccgttcag cccgatcgct gactcaggct cctgtgaccg gatccaggac
    19021 agcagccgcc ggatgtccca caccgcctga gcgacaccgt ggacgttatc gaccacgtcc
    19081 tcgctgggga acgcggcgcc tttgggcagg cctcgtccac gggggccgtg catgggaagt
    19141 acgggaagca tgacattcag gccgagatgc tgatgcagat gccaggctcg aaacagcctg
    19201 agatccaggg cggcacgacc catttccgcg ccgtgcacac acaccagcca cggtcgcggc
    19261 tcccggtgtc gcaacatcag gccgtactcg cgctcgtttg gggtgtagct caaccagcgg
    19321 tcccggccgg gatcacccgc agaaggctca tattcacttt cgaagtagat gcgctcgtag
    19381 gtgcgcccca tgctgctgac gtccctgatg gcaatctcgg tcagtggcgg gggtgccgcg
    19441 aaaaaactct cgggcacttg gagccaaccc ttttcgccat agaactcaag cgccgcgatg
    19501 acctcgagac gtatgcgctc cagtcgcgtc gcgtcgggcg ccgagcgcag caggtgaaaa
    19561 cccaagagca cgatttcgtc gctgaaggcc tgcgccgcga gtgcgagggt aggccgagcc
    19621 acgggaagct cgtcgggttg acgatccaga taggtcgccc acgaccgggc gtagaaacca
    19681 ccggtccggg tgaacggcct tagcgcctgg ccgagcggta aagcccgaac gccccaccga
    19741 ctacggttgc ctcgtcgccg gcggttggca tcgtctggcg cggtgacact tg
//
LOCUS       DASRJA010000642         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1493489,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000642 DASRJA010000000
VERSION     DASRJA010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493489"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFC01_28780"
     CDS             <1..432
                     /locus_tag="VFC01_28780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020105151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN83648.1"
                     /translation="RAVATNGITRFLEPFTIAGYIVMAAAGLSVLNVFVLGLVQRKRE
                     RAALRAIGATPRQEQAVVIAHAGLLGLLVAVLGALGGVGLTYLWSLGSPVYYGIKIDW
                     GVPELALRTGVAAVIVLVLAAAVYPVIHARRLETAEILQTS"
     gene            complement(638..1995)
                     /locus_tag="VFC01_28785"
                     /pseudo
     CDS             complement(638..1995)
                     /locus_tag="VFC01_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601751.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
BASE COUNT          414 a          850 c          752 g          394 t
ORIGIN      
        1 cgcgcggtcg ccacgaacgg aatcacccga ttcttggagc cgttcacgat cgccggatac
       61 atcgtgatgg cggccgctgg cttgagcgtg ctgaatgtgt ttgtcctggg acttgtccaa
      121 cgcaagcggg aacgggctgc gttgagggcg atcggtgcga cgccccggca agaacaggcg
      181 gtcgtcatcg ctcatgccgg acttcttggt ctactggtcg ctgttctcgg tgcacttggg
      241 ggcgtgggcc ttacgtatct gtggtcgctg ggctcacccg tgtattacgg catcaaaatc
      301 gactggggtg taccggaact cgcactgcga accggcgttg ccgcagtgat cgtgttggtc
      361 ctggcggcgg cggtgtatcc agtgatccat gcgcgacggt tggaaacagc agagatactg
      421 caaaccagtt gatgccagcc caatttcgac gcatgtgctg actgacgggc gtgccgcacg
      481 ggcaggtttt cacccccagt agaaccgcgc cgaagccggg ccacattgca gtatcggact
      541 actgcgcttg tcacgccgat tgtcggcaac gattcaattg cgaagcgaga caccgagtcc
      601 ggtggctgtc gttttgccag aacatcgcgg aaggaaagac aattggttcc gcgtgcaccg
      661 tccgcttggc cggggtactc agccggtcga tgcgcgcctc gatggccccc tgcaccgtgg
      721 cccgcacagc gacgtcggcg gcatccaccc gacagacatc gccaccgcgc tcaccggcca
      781 gcaccccgcg ctgcaccatt tcccggacca tctcctcggc gaaaaacgga ttccccgcgg
      841 cccgctcggc gatgaccgcc gccaactcgc cgaccgaggg atccgcaccg agtagctcac
      901 cgatcagtgc tgcggtgtcc gaatcaccca ggggggcaag ggctatcgtc tgctcgccgg
      961 gcacccgcgt cagcgctccc tcatactcgg ggcgggaagt gatcagcacc atcgacgggg
     1021 tgcgcggaat cacggacagg aagtcggcca gcatcgactc gctgaccgca tcgatccgct
     1081 gtgcatcctc gatgatgaac agctccggtg cggtgcgccc cagcgccgcg gtgttgatca
     1141 gcgcggtcag ccggcgccgc cgcgcatccg gttcgatggc gggcgtcggc aactcggggt
     1201 cggcgatgcc gagcagatca tcgacgccgc gaccggccgc cagtgccgtg gcctcccggg
     1261 ccgtgcggct cttgccgatg cccggcggtc ccaccacatt caccacattc accacaccac
     1321 cgcggccacc gatggtgcgg tccaccaggg cgtcgagggc cgccatctcc cagcgccgac
     1381 cgaccaggct ggcctcggcg cgcgcgacca ggccatcgcg cgggccgatc gctagcagtc
     1441 ggcacgcacg cacgggttcg tcggctccct tgatacgcac ccattccggt tcacccaaca
     1501 tcacgttgtg ctggaccagc cgggcggtgg actcgctgag catcacgcca ccaggagccg
     1561 cgaccgattc catccgctgc gccatcccga cctgcttacc gacagcggtg tagcccaacg
     1621 acccgcaccg atctcgccgg cgatcacccg gccggaattc aggcccaccc gcactcgaag
     1681 atcgacaccg tcgcgcgcgg ccacctcggc ggccaaccgc ttcgcctcct cctgaattgt
     1741 taaggccgca aggcaggcgc gaaaagcgtg atcctccaac gccactgggg cgccgaagat
     1801 cgccatcacg ccgtcgccgt ggtactcgac agtgccaccg tagcggcgca gcaccgacgc
     1861 ggaggactcc agcagctcgg ccatgatctc gcgcagtcgc tctatgtcca cggcggctgc
     1921 gatgtccatc gagcgcacca cgtcggcgaa cagcaccgtc acctgcttgt attcggccgc
     1981 tagggccgat ggcatcagtg gtgacccgca ggtgtcgcag aatcgggcgc cctcgtgtgg
     2041 cgcggcgcca cacgactggc acaccgccgg ccctaccgtc accgcatcct ccgccgatcc
     2101 cccgtccgga cggacgtccc gatccgccaa gcgtatggct tctcgggctg attagagggg
     2161 tttctgcacg tggaccgcgg tgacgtgtcg gccgtgcccg ttccgagtag ccaagcatgg
     2221 gcgcccgctg ccaactccgc cgacgcggcg cgcaccaccg aagccgtggc cgttgccgtc
     2281 tggacgagac agctcgagac cgtgactaag tagtaccgcc cggaaaccga gcgaaattgc
     2341 cgtagcccac tactgggctc gtcctacccc gatgcgagtg acgattccca tcgcgggagt
     2401 agacccgacg
//
LOCUS       DASRJA010000643         6640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3465515,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000643 DASRJA010000000
VERSION     DASRJA010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3465515"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..1802
                     /locus_tag="VFC01_28790"
     CDS             273..1802
                     /locus_tag="VFC01_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MorD protein"
                     /protein_id="HZN83649.1"
                     /translation="MADFSDAHAMAVERSCAVTAVALSGQRRQGVRLVTGQSRCFGLS
                     ASLDFVHVPYPSPPDWTRRTLTCGVALQCSPSKERLIEYRLHELSARELRALTLVEAG
                     VALGWIASHWPGLLPELRRVLPDLEVAPGDMDAKEMLNRAIALARAEQQLIIDPLLGR
                     LPSAYTRPQGLSDTLRRTFGRMPWTTTQKRLPRPYSVPVGGDGGVRNPNLPPPCRPQD
                     NDLDITPNHRPGIPYPEWNAWTKRFLPDHVAVLERAHPTRTPQPGSISTDLRKWFEEH
                     THRSMKNRLEDGSDLDVEQYVSHYTDLTTGEAIEPRIFRELLPSSRNVTTALLLDGSS
                     SLGVHGGRIFKLELACADALSRAMALARERHGIFVFSGNTRHRVEVRCLKDFADRRLV
                     PPSGLGLVTGGYTRLGAPLRHLTSRLLAQPSERRLLIVIGDGLLSDEGYEGHYAWADA
                     AHAVEEANDAGVSVYYVGVGPTRVDPLPEVFGPRRSQRIRRIEELPRVLAHVHRELVA
                     A"
     gene            1799..2587
                     /locus_tag="VFC01_28795"
     CDS             1799..2587
                     /locus_tag="VFC01_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HZN83650.1"
                     /translation="MTYYANGNEVQLFEQAYRRRLPVMLTGPTGCGKTRLVEHMALLL
                     GRPMVTISCHDDLTSSDLVGRFMVTGGDVVWTDGPLTRAVKAGAICYLDEVVEARHDS
                     LAILHSLTDHRRALYLDRADEVVQAPDTFMLVCSYNPAYRSSLKELKPSFRQRFVTLP
                     MRYLPAGREAEVIVAETGVELSTARRLVQCAIAIRTADEAFHFEPPSTRVLVTAAQLI
                     ASGTTELEAAYACILAPLSTDGAITDGLREVAAASLLAADASSP"
     gene            2600..2728
                     /locus_tag="VFC01_28800"
     CDS             2600..2728
                     /locus_tag="VFC01_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83651.1"
                     /translation="MDQPERKRKRALIVFQIFIYGYLLIMFGIQLYMSFARGWWEL"
     gene            2815..3420
                     /locus_tag="VFC01_28805"
     CDS             2815..3420
                     /locus_tag="VFC01_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83652.1"
                     /translation="MIVGAAMMGMWAPGLIGFPIFMRGVWLQRQALRAGLSVRPMIVT
                     LIGYLVLIDGCLNSLGWALDLVANHTLINRVLMVGWGNMFDAGYFWHYNELWVGGAAG
                     PGEKAWVAGLILTVFSMRVAAAIGFLQMKRWGHQWMIVTCWMGVVIWIGYVFNMTMYA
                     DVRYAGVVFPVIGWWLYDIFYITPFLAIPYLHTVNREIYSD"
     gene            3442..3870
                     /locus_tag="VFC01_28810"
     CDS             3442..3870
                     /locus_tag="VFC01_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83653.1"
                     /translation="MTTTPESIEQQKAEDLPPGTTPYYANMHKWIKRAVLVCLVALVI
                     EGAFTLPFMAVYYGYPTLSLTEICSELLKVRYSDDTLECKYPYPPLGPPEGAEGKDTA
                     QDEWGIQPVPKYHRLGFRELVRIHEEREARQADQQGAAAP"
     gene            4056..4622
                     /locus_tag="VFC01_28815"
     CDS             4056..4622
                     /locus_tag="VFC01_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isomerase"
                     /protein_id="HZN83654.1"
                     /translation="MAYKIDPDVLHKVAKQVVGLPLEGGELITHAVQMLADEYPGLID
                     PTPGRWVGSKAGGILGKVRFLYFSPREYIVIFGTPTGTQGFSGRYKHVRIHKFLMAGR
                     IDWYDLESDDTAAMTLLPGEHACMEKGQARGLTIHPGSWHIEYGRGAVVTTLPFAMVD
                     TLLVSLEVESVRRSTVEFTKLVRRRLRR"
     gene            complement(4709..5146)
                     /locus_tag="VFC01_28820"
     CDS             complement(4709..5146)
                     /locus_tag="VFC01_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN83655.1"
                     /translation="MAKLSVSVDVPLPPEKAWECASDLSRYKEWLSIHRVWRSKLPET
                     LEKGAQVESIVEVKGMPNRIKWTIVHYKPPEAMTLNGDGKGGVKVKLIGKVKPSGDGS
                     TVTFDVHLGGPALFGPIGMVVAAALKGDIRESLNKFKSVFAQA"
     gene            complement(5153..5332)
                     /locus_tag="VFC01_28825"
     CDS             complement(5153..5332)
                     /locus_tag="VFC01_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HZN83656.1"
                     /translation="MAFLDKVKNLLAKNADKVDTAIDKAGDVVDKKTQGKYTSTVDKV
                     QDAAKKAVDKSNPQT"
     gene            complement(5342..>6640)
                     /locus_tag="VFC01_28830"
     CDS             complement(5342..>6640)
                     /locus_tag="VFC01_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZN83657.1"
                     /translation="KSATKWSGASYGEHGNWLIGAPDVLLEPGSEVAEQAEQIGSLGL
                     RVLLLGASDLPVDHTNAPGTVTPVALVVLEQRVRPDARDTLDYFASQKVTIKVISGDN
                     AVSVGAVAGSLGLGGETMDARQLPDQPEQLADTLEEYTTFGRVRPDQKRAMVHALQSR
                     GHTVAMTGDGVNDVLALKDADIGVAMGSGSSASRAVAQIVLLDNKFATLPHVVGEGRR
                     VIGNIERVSNLFLTKTVYSVLLAVLVGLAGLASKIFGSDPLLFPFQPIHVTIAAWFTI
                     GIPAFVLSLAPNNERAHPGFVRRVMTSALPSGLVVGIATFTSYLVAYQGREATSAEQT
                     QASTAALITLLVTAVWVLAVVARPYQWWRVALVTVSGLAYVVIFSIPLAQKMFMLDSS
                     NMAITSMALGIGVVGAAIIEVIWWVQGALLGERRRLWRTS"
BASE COUNT         1213 a         2198 c         2027 g         1202 t
ORIGIN      
        1 tccggaagtc gacgcgttct acagcgcgac gttcttcctc ctcggcacct acggcgtgct
       61 gaccaccacg gataaccccg acacccgcga cacgatgctg gccgaactgg tcgcatccgc
      121 cgtccgcggc ctcgagctcc gacccatcga caaggaggcc tgaaatggat tggctctggg
      181 aggttcttcg ctacgtcgcg gcatggggcg gcaccggcct gatcatctgg ttctggtact
      241 ggatgttctc caacatcggc accttctgag cgatggccga cttctctgac gcacacgcga
      301 tggccgtcga gcgcagttgc gccgtcacgg ccgtcgcgtt gagcgggcag cgccgccagg
      361 gcgtccgcct ggtgaccggc cagagtcgct gcttcggctt gagcgcgtcg ctcgacttcg
      421 tgcacgttcc gtacccgtcg ccaccggatt ggacccgtcg aaccctgact tgtggtgtag
      481 cacttcaatg ctcgccttcc aaggagcgtc tcatcgagta ccggctccat gagctgtcgg
      541 cacgggaact ccgcgcgctc accctcgtcg aggccggcgt tgctctggga tggatcgcgt
      601 cgcattggcc cgggctgctg cccgaattgc ggcgcgtact gcccgacctt gaggtcgcac
      661 cgggcgacat ggatgccaaa gagatgctca accgcgcgat cgcactggca cgggcagagc
      721 aacagctgat tattgatcct ctactgggca ggctgccgtc cgcctatacg aggccgcagg
      781 gattgtccga cacattgcga cgcaccttcg gcaggatgcc ttggaccaca acgcagaagc
      841 ggctcccgcg cccttattct gtgccggtcg gcggcgatgg cggcgtgcgc aatccgaatc
      901 tgcccccacc gtgcagacca caggacaacg acctcgacat caccccgaac caccgacccg
      961 gcatcccata tcccgagtgg aacgcatgga ctaagaggtt cctaccggac cacgtcgcgg
     1021 tactcgagcg agcacatccc acccgcacac cacagccagg ctccatctct accgacctcc
     1081 gcaaatggtt tgaggaacac acccatcgct ccatgaagaa ccgcctcgaa gacggctccg
     1141 atctcgacgt cgagcagtac gtcagccatt acaccgacct gacgacaggc gaagcgatcg
     1201 agccacgcat ctttcgtgaa cttctgccca gcagccgcaa cgtcaccacg gcgctgctcc
     1261 tcgacggcag ctcctcgttg ggcgtgcacg gaggcaggat attcaaactc gaattggctt
     1321 gcgccgacgc gctttcccga gcgatggcgc tcgcccgcga gcgccacggc atcttcgtct
     1381 tcagcggcaa cacccgccac cgcgtcgaag tccgttgcct caaggacttt gcggataggc
     1441 ggctcgtgcc gccgagcggc ctcggcctgg tcaccggcgg atacaccagg ctcggcgcgc
     1501 cgttgcgcca tttgaccagt cggctcttgg cgcaaccgtc cgagcggcgc ctgctcatcg
     1561 tgatcggtga tggcctgctc tccgatgagg gctacgaggg ccactacgcc tgggccgacg
     1621 cagcgcacgc cgtcgaagag gccaacgacg ccggcgtgtc cgtgtactac gtcggcgtcg
     1681 gaccaacccg cgtcgatcca ttgccggagg tcttcggtcc ccggcgctcc caacgcatcc
     1741 ggcgaataga agaacttccc agggtattgg cccatgtcca tcgagagttg gtggccgcat
     1801 gacttactac gcgaacggca acgaggttca gttgttcgag caggcgtacc ggcggcgtct
     1861 gccggtgatg ctgaccggcc cgacgggatg cggcaagaca cgcttggtcg agcacatggc
     1921 tctgctgctc ggacggccca tggtcaccat cagctgccat gacgatctga ccagttcgga
     1981 cctcgtgggt cggttcatgg tgaccggcgg ggatgtcgtg tggaccgacg ggccactcac
     2041 cagagcggtc aaggccggag ccatctgcta tctcgacgag gtggtggagg cgcgccacga
     2101 ctcgttggcc attctgcact cgctgaccga tcaccgtcgc gcgttgtatc tggaccgcgc
     2161 cgacgaagtt gtccaggcgc cggacacatt catgctcgtg tgctcctaca accccgccta
     2221 ccgcagctcg ctcaaggaac tcaaaccgtc gttccgtcaa cggttcgtca cgcttccgat
     2281 gaggtacctg ccggccggcc gcgaggccga ggtgattgtc gccgaaacgg gtgtcgagtt
     2341 gtcgacggcc cggcggcttg tccagtgcgc gatcgcaatc cggaccgcgg acgaggcgtt
     2401 tcatttcgaa ccgccgtcga cgcgagtgct ggtcaccgct gcgcagctga tcgcctccgg
     2461 cacaaccgaa ttggaagcgg cctatgcctg catcctcgcc ccgctgtcca ccgatggggc
     2521 gatcaccgac ggcctgcgtg aagtggcagc ggccagcttg ctcgccgctg atgcctcatc
     2581 cccctaggaa ggaacggcaa tggatcaacc agaacgcaag cgcaagaggg cgctgatcgt
     2641 cttccagatc ttcatctacg gctacctgct gatcatgttc ggcatccagc tctacatgtc
     2701 gttcgcgcgc ggctggtggg aattgtgaac gtaggactgc agaccggcgg cgccgtcagc
     2761 ctcgacgatc accacgaaga gtcgcgcctg gctcagcgca gggccgacaa gtggatgatc
     2821 gtcggcgccg cgatgatggg gatgtgggca ccggggctga tcgggtttcc gatcttcatg
     2881 cgcggggtct ggttgcagcg ccaggcattg cgcgccgggt tgtcggtccg gccgatgatc
     2941 gtcaccctga tcggctacct ggtcctgatc gacggatgtc tcaacagcct cggttgggca
     3001 ttggacctgg tcgccaacca caccttgatc aaccgcgtgc tgatggtcgg gtggggcaac
     3061 atgttcgacg ccggctactt ctggcactac aacgagctct gggtcggtgg cgccgcggga
     3121 ccgggcgaaa aggcttgggt cgcagggctg atcctcacgg tgttctcgat gcgagtggcc
     3181 gcggcgatcg ggttcctgca gatgaagcgg tggggtcacc aatggatgat cgtcacctgc
     3241 tggatgggcg tcgtgatctg gatcggctac gtgttcaaca tgaccatgta cgccgacgtc
     3301 cgctacgccg gtgtggtgtt ccccgtgatc ggctggtggc tctacgacat cttctacatc
     3361 acccccttcc tcgccatccc gtacctgcac acggtcaacc gcgaaatcta ctccgactga
     3421 acaggtttaa ggagcaccgc tgtgacaaca acacccgaat ccatagaaca acagaaggcc
     3481 gaagatctac ctccgggtac cacgccctat tacgcgaaca tgcacaagtg gatcaaacgt
     3541 gcagtgctgg tttgcttggt cgcgctggtc atcgaagggg cattcacgtt gccgttcatg
     3601 gcggtctact acggctaccc gacactgagc ctcaccgaga tctgcagtga actgctcaag
     3661 gtgcgctact cggacgacac cttggagtgc aagtacccct acccaccgtt gggtcctccc
     3721 gagggcgctg aaggcaagga caccgctcag gacgaatggg gcatccagcc ggtgccgaaa
     3781 taccaccggc tcggcttccg cgagctggtg cgtatccacg aggagcgcga ggcacggcag
     3841 gccgaccagc aaggcgctgc ggctccgtga cctacaacga cctgggaaga aagccaacgt
     3901 tgttccccgg cgcgcgtgct gcgtctcacg cactttacgt tccaccatct gatgggacgc
     3961 cgtaccgctg tgcgcctgat ggcctttgcg agcaatcgag ctaataaaga gcagggcagc
     4021 tacagcacgg agaccgcggt taggcagatc agctgatggc atacaagatc gaccccgacg
     4081 tcctgcacaa ggtcgccaag caagtcgtcg gccttccgct ggaaggcggc gaactcatca
     4141 cacacgccgt ccagatgctc gccgacgaat accccggcct catcgatccg acgccggggc
     4201 gttgggtcgg cagcaaggcg ggcggcatcc tcggcaaggt ccgcttcctc tacttcagcc
     4261 cgcgcgagta cattgtgatc ttcgggactc ccactggcac acaaggcttt tcgggacgct
     4321 acaagcacgt gcggatccac aagttcctga tggcgggccg aatcgactgg tatgacctgg
     4381 aatccgacga caccgcggcc atgacgctcc taccgggcga gcacgcgtgc atggagaagg
     4441 gtcaagcgcg gggcctcact attcaccccg gttcgtggca tatcgagtac ggccgcggcg
     4501 cggtcgtgac gacgctgccg tttgcgatgg tggacacgct gctggtgtcg ctggaagtcg
     4561 aatccgtgcg acgctcgacc gtcgagttca ccaagctggt cagaaggcga ttgcgccgct
     4621 gacattcgag cgcgaacaga cgcaaaactg cccaaaattg gtcgaaaata ggcaagtttg
     4681 cgtctgctcg ccagagaagg tgactccctt acgcctgggc gaaaacggat ttgaacttgt
     4741 tcagcgactc gcggatatcg cctttgagcg cggcggcgac caccatgccg atcgggccga
     4801 acaacgcagg cccgccgaga tgcacgtcga atgtgacggt cgaaccgtcc ccagacggct
     4861 tgaccttgcc gatcagcttg accttgacgc cgcccttgcc gtccccgttg agcgtcatcg
     4921 cctcaggcgg cttgtaatgc acgatggtcc acttgatccg gttgggcatg cccttgacct
     4981 cgacgatgga ctcgacttgc gcgccctttt ccagggtttc gggcagcttg gaccgccaga
     5041 cccggtgaat cgacagccat tccttgtacc gagacaggtc agaggcgcac tcccacgcct
     5101 tctccggcgg cagtggaacg tcgacggaga cggaaagttt ggccatacgt cgttaggtct
     5161 gcgggttgct cttgtcgacg gccttcttgg cggcgtcctg caccttgtcg accgtcgatg
     5221 tgtacttgcc ctgcgtcttc ttgtcgacga cgtcaccggc cttgtcgatc gcggtgtcca
     5281 ccttgtcggc gttcttggcc agcagattct tcaccttgtc gaggaatgcc atgggtccac
     5341 cctaactagt tcgccacaat cggcgtcgtt cgcccaacaa ggcaccttgt acccaccaga
     5401 tcacctcgat aatcgccgca ccgaccacgc cgatgcctag cgccatcgag gtgatcgcca
     5461 tgttcgacga atcgagcatg aacatcttct gagctagcgg gatggaaaag atcaccacgt
     5521 aggccaggcc cgacaccgtg acgagcgcga cccgccacca ctgatacggc cgcgccacca
     5581 ctgccagcac ccacaccgcg gtcaccagca gcgtgatcag cgccgccgtc gacgcctgtg
     5641 tctgctcggc ggacgtggcc tcacggccct ggtatgccac caggtaggac gtgaacgtcg
     5701 caatgccgac cacaagcccg gacggcagcg ccgaggtcat cacccgccgc acgaagccgg
     5761 gatgcgcccg ttcgttgttc ggcgccaacg agaggacgaa cgccggaatg ccgatggtga
     5821 accatgccgc gatcgtcacg tggatcggct ggaacgggaa caagagcgga tcagacccaa
     5881 atatcttgga cgccaggccc gccagtccca ccaggaccgc gagcagcacg gagtacacgg
     5941 tcttggtgag gaacaggttg gacactcgct cgatgttgcc gatcacccgg cggccttcgc
     6001 cgacgacgtg gggcagcgtc gcgaacttgt tgtccaacaa cacaatctga gcgacggcgc
     6061 gcgacgcgga gctgcccgag cccatcgcca ccccgatgtc ggcgtccttg agcgccaaca
     6121 catcgttgac gccgtcaccg gtcatcgcga ccgtgtgccc gcgtgactgc agcgcatgca
     6181 ccatcgcgcg cttctggtcg ggacgcaccc ggccgaacgt cgtgtactcc tcgagcgtgt
     6241 cggccagctg ctccggttgg tcgggcagct gccgggcatc catcgtctcg ccccccaggc
     6301 cgagggagcc tgccacggcg cccaccgaca ccgcgttgtc gccggagatc accttgatcg
     6361 taaccttttg agaggcaaag taatccagcg tgtcgcgggc gtctgggcgg acgcgctgct
     6421 cgagcaccac cagcgccacc ggcgtgacgg tgccgggggc gttggtgtgg tcgacgggca
     6481 ggtcactcgc gccgagcagc agcacccgca ggcccagcga cccgatctgc tccgcttgtt
     6541 cggcgacctc tgaccccggc tcgagcagca cgtcgggcgc gccgattaac cagttgccgt
     6601 gctcgccgta cgacgcgcca ctccatttgg tggcggactt
//
LOCUS       DASRJA010000644        42159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4138793,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000644 DASRJA010000000
VERSION     DASRJA010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 42159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42159
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4138793"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VFC01_28835"
     CDS             complement(<1..595)
                     /locus_tag="VFC01_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010541240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5701 family protein"
                     /protein_id="HZN83658.1"
                     /translation="MSSVREQAERLIELGVDRMAGLPADRLRNLPDSSDALLVVHPDL
                     TPPSRLAPLLRVNDKPGFVVTDMVDVDEFAPIPSVKLPDAPLYLLRDLDRGDAMANWS
                     PDEALPAIIEAGRTPLTLSEGVHWLLQRPDVLARGRCFMTIGSRRRKAAGSLDARTPA
                     VWISTGTGRDGPANRDAPKVGWCWAGNRHTWLGFASAS"
     gene            705..2159
                     /locus_tag="VFC01_28840"
     CDS             705..2159
                     /locus_tag="VFC01_28840"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HZN83659.1"
                     /translation="MAVNAHGPSIGPSVAEILASHAGGTGSPTKTAARVADAIAARGD
                     DGTWLSIVPRDQLLVTAEEIEKRPGARTLPLYGVPFAVKDSIDVEGVPTTLSCPDYAY
                     VSSTTAPVVRRLLDAGALYVGKTNLDQFATGLNGTRTPYTIPRSVYGGDMISGGSSSG
                     SALAVALGQVPFAVATDTAGSGRVPAALNGVVGFKPSRGLISTVGLVPACRSLDCISL
                     MAGAIDDVDRVFDVVAARDDEDAWSRDRGPRYGGSSIRVGLPPSDELDFFGDEEMRRA
                     HLAFREQLDRTATVVDVSLKPFLAAGALLYQGPWVAERLVEFGDFLAAQPDSIHPVVR
                     EILRGGQRYTAVDAFAALQRLAELKALVARLWQHMDVLVVPTIGTTFTVDEVLAAPIE
                     TNTMLGHYTHFGNLLDLSGVAVPLGITSDGRPYSAMLLGAALTDDTVLHLAAQVLDEP
                     RQVRPSAVSTMTEEPYEPTHRSARRTVAVPARRG"
     gene            2101..2793
                     /locus_tag="VFC01_28845"
     CDS             2101..2793
                     /locus_tag="VFC01_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HZN83660.1"
                     /translation="MSQPIEVPAEPSPFRLVAGKTALIVIDMQRDFLLPGGFGESLGN
                     DVDQLLKVVPPLADLIAAARSAGIMVIHTREGHEPDLSDCPPAKLNRGEPSKRIGDEG
                     KYGRILIRGEYGHDIVDELAAIDGEVVIDKPGKGAFYATELQDVLTAAGITQLLVTGV
                     TTEVCVHTTTREANDRGYECLVVSDCVGSYFPEFQRIGLEMIKAQGGIFGWVADTAAV
                     IPALHALTTTAA"
     gene            2806..4407
                     /locus_tag="VFC01_28850"
     CDS             2806..4407
                     /locus_tag="VFC01_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HZN83661.1"
                     /translation="MTTDVTKPPADPPVDPPAVATSLPLWTRGDTNAFFGLGFNILVN
                     VLTLTGLMIGVVSVPAGDVLGTVLPALGVALILGNLYYTFLARRLARRENRDDVTALP
                     YGPSVPHMFIVVFVVMLPVYLNTKDPIQAWSAGLAWAFMIGVIVMIGAFVGPYIRKLT
                     PRAAMLGTLAGISITFISMRPAAQMWEAAWIGLPVMAIILIGFFTNVKLPFGIPVGLA
                     ALLVGTAIGWIGGFMSAPDVGQAISDIAIGIPDLRLDLLFGGLSNLAPLLGTAIPLGV
                     YNFTEAMSNVESAAAAGDNFNLRSVLLADGAGAVIGSAFGSPFPPAVYIGHPGWKDAG
                     GRANYSLASGLMIGILCFLGLFGVLDALLPVPAIVPILLYIGLLIGAQAFQAVPRLHA
                     VAVVAALLPNLAQWASGLIDNALNAAGTSAAKVGLEALNGAGVVYDGLQTLGEGAVLV
                     GLILGTMVTFILEKKFLYAGIASFVGAVLSFIGLIHAPEVAWAATPQVALGYVFFGIV
                     CVAYSFLPGAKDPVDVDESDIVAGH"
     gene            complement(4714..5763)
                     /locus_tag="VFC01_28855"
     CDS             complement(4714..5763)
                     /locus_tag="VFC01_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016881468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZN83662.1"
                     /translation="MHARSVLGCGLDGDTGEVFQRRLTPDHGEIVAWLGDLPSPVAVT
                     YEAGPTGFGLARALTAAGISCEVAAPSKLIRPAGDRVKTDTRDAAHLARLLHLGQITA
                     VTVPTAAQEAARDLVRAREDCRGDLMTARHRVSKLLLRQGIVYFGGKAWTSRHELWLR
                     AQRFDNPALQLSYEAAFDAMLACLDRRDRLDDAITAMAADSEFTPVVHRLGCIRGIAT
                     LTAFGLAVEFGDWHRLTGRSIGAYVGLVPSEHSSGDSRAQGGVTKTGNGHARRLLVEA
                     AWHHRSPYRPGAELRRRWDLASPAARARGQAANRRLHTRWVGFDARKKRSVIANVAIA
                     RELAGWCWLLAVMPD"
     gene            complement(5884..6504)
                     /locus_tag="VFC01_28860"
     CDS             complement(5884..6504)
                     /locus_tag="VFC01_28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HZN83663.1"
                     /translation="MLQTILNYAGIAVFASSGAVVGVRKGFDVFGIATLGVLTGVGGG
                     ILRDLLLDRPPYSIQHWPNITVALIAVAIATPFAHIVIRMNTVVLVLDAIGMGFFATS
                     GAAIAVDEGASWFAAGLIGMLTAIAGGMLRDVIAREVPLVMGPDDLYAIPAMLGAATY
                     VAIDYFWPQWIAIAVGSAVATALRLAGLAFHWRLPTGPPELITPYD"
     gene            complement(6514..6720)
                     /locus_tag="VFC01_28865"
     CDS             complement(6514..6720)
                     /locus_tag="VFC01_28865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83664.1"
                     /translation="MRQLVVTLAGTLIFSTRDGEEFTLRPGDVLLAEDTAGSGHQWRL
                     QGSDPWRRMYVVLANGADVPFVAD"
     gene            6939..7286
                     /locus_tag="VFC01_28870"
     CDS             6939..7286
                     /locus_tag="VFC01_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZN83665.1"
                     /translation="MNTATVVVTILLAALFTFSASIKLLGVRQSLAIRDRLGVKPLQW
                     RLIGVCELAGVAGVLVGLLWTPIGIAAAIGLALLTIGAIAYHVRVSDTVADLAPAVIG
                     VLLALTAVVLHTL"
     gene            7377..7967
                     /locus_tag="VFC01_28875"
     CDS             7377..7967
                     /locus_tag="VFC01_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN83666.1"
                     /translation="MAARAHSVDPRAERVRTRLREAAFALAHEGPVDELTVGDVVARA
                     EVSRQVFYQHFRDRDDAVATAFTVAFAAATADIDTDARARILRLFDFAAEHRSMYRNI
                     VPSAVTQRVVTAFRAELQPACVQIATQGMPVVSPIASLTPDSVSRFLVGGFMEVLRSW
                     MEDPDSTDLGARVSAALDTVNALLGLTTSSKGQQHG"
     gene            7960..9015
                     /locus_tag="VFC01_28880"
     CDS             7960..9015
                     /locus_tag="VFC01_28880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83667.1"
                     /translation="MAKHQAPERHPVRDRAAVRTTALAMIGLTLFVIAMIASYSGAFA
                     KPTLHHMAVAVAGPQQVVDGIRGQESLAVTEVGDDAAARQQVYERKTDAAFVVAPTGD
                     MKIYVAGGGGRSVANAAETVGRTIAAKTGLKPSVEDVAPASAGDPSGTIEFYAIIFIS
                     IGASVGATVLGRLMGAVRRPLTLALRTATLAGFAAVLAGAVTVYVDMILGALTAHTWH
                     LFGALWLYAMAVAGAITGVAAAFGSVASMALTAFLVVIGNAAAAGPVGRPLLSGFYST
                     FTTIVPQGSGVSLLRSVEYFGGNGAQTPLVTLAIWGAAGCLLAIIATASRVNYRAVYE
                     RFAGRTLLRPRVLSPAD"
     gene            8957..10633
                     /locus_tag="VFC01_28885"
     CDS             8957..10633
                     /locus_tag="VFC01_28885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN83668.1"
                     /translation="MSVSLAEPYYDLGSYHRPIETASHEAQLWFDRGMVWAYAFNHEE
                     AIRCFDRALELDPDLAIARWGVAYSIGPNYNKAWEAFDPADLAASLARARMELGLAAK
                     GRASAVEHGLIEALQARFPTDDPNDPDALQAGHTAYADAMVALAEAYPDDIDVQALTA
                     DALVNITAWALWDIRTGEPAPDSRVVEAKRILDGMLATPEGRKHPGVLHLYLHTMEMS
                     GTPQDALPAADLLRGLVPDAGHLEHMPSHIDVLCGNYRDSVVANQSAVQADRRFVERE
                     GPLHFYSLYRAHNLHFIVYSAMFEGNSRAAFDAAEELAGQLTPELLAIESPPMADWLE
                     AFVPLKIHVLVRFGRWDELIALPLPDDVELYSCTATTIHYGRGVAHAAKGQLPQAHAE
                     RDAFAEAYARIQDTRYLFNNTARDILAIAAEMLDGEIDYREGRFEDAFAHLRRAIELD
                     DNLPYDEPWGWMQPTRHAYGALLLEQGRVEEAAQVYAADLGLDPTLARPCQHPGNVWS
                     LHGYHECLQKLGRTDEAALIGQQLELLKARADVAITASCACRLEVAEPCH"
     gene            complement(10637..11038)
                     /locus_tag="VFC01_28890"
     CDS             complement(10637..11038)
                     /locus_tag="VFC01_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012687674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN83669.1"
                     /translation="MSTGIWRPTSTDDAVVRYQIADLDRGIEFYTNQLGFRLAQRAGP
                     IAVVARGALNLLLSAPASSGAQPLADGQTQQPGGWNRIVLYVDDIAADVDRLRNAGVT
                     FLNDVREGPGGKQVQITDPDGNSIELHEAPA"
     gene            complement(11048..11899)
                     /locus_tag="VFC01_28895"
     CDS             complement(11048..11899)
                     /locus_tag="VFC01_28895"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN83670.1"
                     /translation="MQYRTYRPAPPLADFVAYMWTMRDLAVHAHERIVPSGTLELVIN
                     LNEDALRIYDPGSGVWREYSGAVVSGAYRQYFVIDTRDHASIVGVHFKPGGAWPFLGV
                     PPGELTDRHIDLAMLWGSSAVELRERLCAAPTSQQRFALLEQTLRSCLAEAQPGHPAV
                     PFALGRLARPGVTVGAVAEDVHLSRRRFIQVFTSEVGMTPKRLSRVLRFQRASALARR
                     INAPDWARLARACGYFDQSHLINEVSEFTGTSPSRLRPASESVKELHLAVPDEVKFLQ
                     DADQASP"
     gene            complement(11917..12687)
                     /locus_tag="VFC01_28900"
     CDS             complement(11917..12687)
                     /locus_tag="VFC01_28900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HZN83671.1"
                     /translation="MRLGFDRHCAEIVTQTALLADHLNGADVTVPVPTCPGWNVSQLA
                     RHVDGGQRWADAIVATRASEPPSDAALRDLSGATNDDPAVLAASLTQAATALRATLTE
                     AGPDAQMWCPVGDGGSAFYARRFAHETALHRADAALALGVDYTLEPDVAADAVDEWMD
                     LGCLPFHFEVHPWMRELLAPGRTIGLHATDTDAHWLLDLTGDVIAWRGADEPATAEIR
                     APVTDLLLAIYRRRPVAGLEIAGDATLVDFWLERVAFG"
     gene            complement(12684..13040)
                     /locus_tag="VFC01_28905"
     CDS             complement(12684..13040)
                     /locus_tag="VFC01_28905"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN83672.1"
                     /translation="MSQQKAVVEKYTDGFRRGDLAQILSCLTDDVVWALHGAKTLVGK
                     DAFAAEADSGDGPNPELTLDRLMEEDDAVAVIGHGSVSLGGDPHAFVYSEVFTFADGL
                     VSRLDTFHIWLGGPPA"
     gene            13282..13761
                     /locus_tag="VFC01_28910"
     CDS             13282..13761
                     /locus_tag="VFC01_28910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZN83673.1"
                     /translation="MAISDDDLDRLRRCVELARVALDDGDEPFGSILVDGDGATLYED
                     RNRVKDGDQTRHPEFAIARWAVDNLTPDERARATVYTSGEHCPMCAAGHAWVGLGRIV
                     YATSSEQLTQWLAEWGAPPPPVAPLPIATVALRVDVDGPAPELADTMKALYEAKFRP"
     gene            13758..15035
                     /locus_tag="VFC01_28915"
     CDS             13758..15035
                     /locus_tag="VFC01_28915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HZN83674.1"
                     /translation="MSEPGWIEHAIWWQVYPLGFVGAYPADPPPTPDEHRLRRIVDWL
                     DHAIELGTSGIALGPVFASRTHGYDTTDHYSIDPRLGDDADYDHLIAEAHRRGLRVVL
                     DGVFNHVAGDFARPEWFRKHGKDFDVFEGHGELIALDHDNPDVVAYTVDVMTHWLRRG
                     ADGWRLDAAYAVPDRFWAQVLPRVRQEFGDAWFVGEVIHGDYIARVRESGFDSVTQYE
                     LWKAIWSSLNDGNFHELDWALLRHNEFLDDFVPMTFVGNHDVTRIASQLANTSHLEHA
                     LVILFTTGGTPSVYAGDESAYRGVKEERFGGDDAVRPEFTAPLMGVDDLGHDVFRLHQ
                     YLIGLRRRHPWLHTARTSPLRLTNTQYVYETRNSDDALLVALNIDEAPLPLSLPELGF
                     GQGEIVAGSGAPPQSVVADAEIAPHGWLIIAPR"
     gene            complement(15019..15483)
                     /locus_tag="VFC01_28920"
     CDS             complement(15019..15483)
                     /locus_tag="VFC01_28920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HZN83675.1"
                     /translation="MGKSLSSKAADPFDLQRFVDAQDRVYPTVLDELRGGRKRSHWIW
                     FVFPQLRGLGRSQTALRYGISSLEEAQAYLAHDTLGPRLRECTRLVAAIDGSSADDVF
                     GWPDNLKVRSSMTLFARATDDPDVQADFRAVLDKFYDGAEDSATVDLLSAAR"
     gene            15642..17696
                     /locus_tag="VFC01_28925"
     CDS             15642..17696
                     /locus_tag="VFC01_28925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN83676.1"
                     /translation="MLLVTSILSAAVVGAIGYQSGRSSLRASVFDALTEIRQSQTRQL
                     QAQLTDLQNSLVVFTRGSTAIEATEAFTAAFDQLNNATITPAQQQSIVDYYTKEFAPA
                     EDEQTGDDVDIDALLPTTNAQKYLQANYTTPFTDFDQALSFDDAHDGSAWSAASAKFN
                     DFFRVIVKRFEFEDALLLDTRGNVVYSAYKGVDLGTNILTGPYKAGDLTDAYNKALDS
                     HTVDYVAVTDFSDYQPADEPTAWMVSPVGPQNRVEGVLALQFPISKINRLMTMDKRWE
                     ESGMGKTGETFIVGPDDLMRSDSRLFLEDPEAFKRDVVNAGTPPDVAEESIRQHGTTL
                     VQPVATEATQRAQRGQKGTLVADDYLGHETLQAYAPVDVSGLHWSVIAKIDTSEAFAP
                     VSAFTRTLVLSTVVIIFIVCLAAMLLARLFVRPIRRLEAGAQRISSGDYDVAVPVQSR
                     DEFGDLTVAFNDMSRNLAIKEELLTEQRRENDRLLLSLMPEPVVQRYREGEETIAQDH
                     QNVTVIFADIVGLDELSAELTSDELLTIVNKLTRQFGAAAENLGVEQVRILHNGYLAS
                     CGMSVPRLDNVRRTVDFAIEMQRIIDRFNKETGNDLTLRAGIDTGTVTSGLVGRSTLA
                     YDMWGSAVNLAYQAQSGAPQPGVYVTSKVYEAMRDSRDFTSAGVITVDGEEQPIWRLG
                     QS"
     gene            17693..19093
                     /locus_tag="VFC01_28930"
     CDS             17693..19093
                     /locus_tag="VFC01_28930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZN83677.1"
                     /translation="MRSVLDAPWFYWAVGVAIGFPILLVLLTELQHALRRRGSILTRP
                     VYLLRTYILPLGALLLLLVQAMQISGEETPVRVVATVFGFVVLVLLLSGLNATVFQGA
                     PEGSWRKRIPSIFLDVARFALIAIGVGLIFAYIWGAHIGGLFTALGVSSIVLGLTLQN
                     SVGQIISGLLVLFEQPFQLGDWIQTPTARGRVVEVNWRATHIDTGRGLLIMPNSVLAA
                     ASFTNFSRPPGAHSLSVTTTFAVDDPPDDVIAMLNRVAAMVPQLRPDAAPSSVHSGGL
                     QYSTGIPLRSPADDFATKSMFLRWVWYASRRAGLHLDEAEDEFSTPERLKASLRIIAP
                     TLRLNQAEQQTLLPHARITRYGSDEYLQFAGQVPKRMSFVVNGRVRLVVTGDDGAVLP
                     VRTLEEGDFIGQTALTREPVAAAAYALEEVTVLQIEREYIEELVARKPLLLQDIGRAI
                     EERRANVRRVLAAAGE"
     gene            19100..20344
                     /locus_tag="VFC01_28935"
     CDS             19100..20344
                     /locus_tag="VFC01_28935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZN83678.1"
                     /translation="MNRRVPRPSELAPLIGFKKPQLNATKRRLASALTIDDLRRIAKR
                     RTPKAAFDYTDGAAEDELSLARARQAFRDIEFHPTILRNVEKVDTSRTVLGDRVALPF
                     GIAPTGFTRLMQTEGEIAGAHAAARAGIPFSLSTLGTSSIEDVKTANPHGRNWFQLYM
                     WKDRDRSMALVERAAAAGFDTLLVTVDVPVAGARLRDTRNGMSIPPTLTLRTVLDAVP
                     HPRWWFDLLTTEPLSFASLDRWPGTVAEYLDTMFDPTVTFEDLAWIKTQWPNKLVVKG
                     IQTLEDARAVVDLGVDGVLLSNHGGRQLDRAPVPFHLLPTVARELGESTEILLDTGIM
                     SGADIVAAIALGARFTLVGRAYLYGLMAGGEAGVDRAITILSEQVTRTMRLLGVTSLD
                     ELAPHHVTQLYRLGRAVPRAGG"
     gene            20545..21540
                     /locus_tag="VFC01_28940"
     CDS             20545..21540
                     /locus_tag="VFC01_28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HZN83679.1"
                     /translation="MKFMEKFRGKWLRRLAVGAVAAATLPGLIGFVGGSATASAFSRP
                     GLPVEYLDVFSAAMNRNIRVQFQGGGPHAVYLLDGLRAQDDYNGWDINTPAFEWYNGS
                     GLSVVMPVGGMSSFYTDWYQPSRGNGQDYTYKWETFLTQELPAWLEANKGVSQNGNAV
                     VGLSMAGSTALTYSIYYPQKFIYAASLSGFLNPSEGWWPMLIGLAMNDSGGYNAESMW
                     GPSSDPAWKRNDPMVNIAQLVANNTRIWIYCGTGTPSDLDSGTSGENLMAAQFLEGFT
                     LRTNVTFRDNYIAAGGTNGVFNFPSQGTHTWAYWGQQVQQMMPDIQRVLGAQASA"
     gene            21671..23995
                     /gene="lon"
                     /locus_tag="VFC01_28945"
     CDS             21671..23995
                     /gene="lon"
                     /locus_tag="VFC01_28945"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZN83680.1"
                     /translation="MAEAKSVPVLFLSDSIVLPGMVVPIELDDAARAAVDAARASESG
                     ELLIAPRLEDRYPSYGVLASIVQVGRVAGSSGTAAVVRGNGRAQIGAGANGPGPALWV
                     EVTEVEEAEATAETTAMAAEYKKLLLAYLQRREAWQIVDFVNQLTDPSALADMAGYAS
                     YLTQVQKRQLLETPDVAERLRVLIDWTSDHLAEVEVNDKIAEDVREGMEKTQKEFLLR
                     QQLAAIRKELGEGEPDGSDDYRARIEAAELPEKVREAALREVGKLERSSEQSPEGGWI
                     RTWLDTVLDLPWNVKTEDSTDLKAAREILDADHHGLEDVKDRIVEYLAVRARRAQRGM
                     AVVGGRGSGAVMVLAGPPGVGKTSLGESVARALGRKFVRVALGGVRDEAEIRGHRRTY
                     VGALPGRIVRAIGEAGSMNPVVLLDEIDKVGSDYRGDPSAALLEVLDPAQNHTFRDHY
                     LELDLDLSDVVFLATANVIENIPSALLDRMELIQIDGYTEDDKVAIARDFLLPRQRDR
                     AALTEDEVTVTEDALRKIAADYTREPGVRQFERLLAKALRKATTKLAENPEVLTIDEP
                     DLVDYLGRPRFMPESAERTAVPGVATGLAVTGLGGDVLYIEAGATDGEPGLQLTGQLG
                     DVMKESAQIALSYVRSHAEQLGVDPKALDRRIHVHVPAGAVPKDGPSAGVTMVTALVS
                     MATGRQVRSDVGMTGEVTLNGRVLPIGGVKQKLLAAQRAGLSTVFIPQRNEPDLDDVP
                     AEVLEALEVKPMTDVADIVALALEPAADAATVAA"
     gene            complement(24080..24400)
                     /locus_tag="VFC01_28950"
     CDS             complement(24080..24400)
                     /locus_tag="VFC01_28950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83681.1"
                     /translation="MTILREWRGELRRELVGEYVAYMRDTGLADYRATPGNLGALIAV
                     RDLDDERAEVITLSWWPSMDAIRGFAGDDVERAVYYPEDDRYLLTKPEGVQHYESHGA
                     FDAP"
     gene            24534..25559
                     /locus_tag="VFC01_28955"
     CDS             24534..25559
                     /locus_tag="VFC01_28955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN83682.1"
                     /translation="MPKRGKPLRKLGFLTIGRFDAADPGPGHEQTLSMIERAEALGFD
                     SVWLRCRHLQPGISSPVAMMAAASQRTTHIELGTAVIPLGLENPLRLAEDLATVDILS
                     GGRINPGVSVGTPMLYDNYKTTLYPETHELENFSKDRVVRLLECLRGEPVSDFEGKVG
                     IEDFTRRVQPHSAGLADRVWYGGGMSSAIWAGQQGINYMTSSVVSIEGTASRDFATIQ
                     GENIDAYRAHHPEPERARVSQGLVVIPTDSASPEQVEKYRAYAASRFDRTKAPQGARG
                     MLFSPDYVGTSDELADTLYAHAGFQRADEIAFALPFTFDEEDYVQIVTDMAEKLGPKL
                     GWTPAQA"
     gene            25589..25921
                     /locus_tag="VFC01_28960"
     CDS             25589..25921
                     /locus_tag="VFC01_28960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HZN83683.1"
                     /translation="MAYDEDLADRIRELIAAERGVEEKRMFGGLAFLINGNMSVAASG
                     KGGLMVRVPPDETEKLLARDHVEPMVMAGRETRGWLRVSGDGVKSKRQLQSWVGRGVD
                     YARSLPPK"
     gene            complement(25926..26489)
                     /locus_tag="VFC01_28965"
     CDS             complement(25926..26489)
                     /locus_tag="VFC01_28965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83684.1"
                     /translation="MYSAHQYFDQTGRYTVAGNGPLELRYSFWANRFEAKSTTGERSF
                     DEWNLHRLDKFIDWLARNQVRGDIGEIGWPSYQQMVASGIPASEASAESQRWNVLAEA
                     WFQKADAASLSVTYFAASGLQFVDHPGLPPRLPEPNAVFVHSAGNGELRDAMGMPILT
                     PEGKFQPRDIDTANSQYDVLIKHPSRD"
     gene            complement(26452..27054)
                     /locus_tag="VFC01_28970"
     CDS             complement(26452..27054)
                     /locus_tag="VFC01_28970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83685.1"
                     /translation="MSVRVRWSLVVSIGVLAGVAMYVASVARGTGAGDMAWPLQAARD
                     IIAGHDPYVQPVTVDDAGNLWPTNPVTTAMPFLPFTWAPDAVIAAVFFGAMSALLAYG
                     LTKEGKLWPLLTFASPAYVACVVYVQWAPLILATYYLPQLLPLALVKPSIALPVLLTR
                     LNLKRALACGYVRRRSSQGVDQRSARQGGVLGAPVLRPDG"
     gene            27222..27959
                     /locus_tag="VFC01_28975"
     CDS             27222..27959
                     /locus_tag="VFC01_28975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZN83686.1"
                     /translation="MRIGTSGWSYDHWAGVLYRPGLPVAKRLARYVEEFDTVELNASF
                     YRWPKDATFSGWRERLPEGFTMSVKAHRGLTHYRRLGSPEPWVERFERCWTALGDRRE
                     ALLVQLHPGMERDDARLDHFLTVMPDWIPVAMELRHPSWDDPAVYATLERHHAAYVVM
                     SGAKVATISRATADLVYVRMHGPAQDSMYHGSYPDDELRQWADRVAEWHDEGRRVLVY
                     FNNDLGGHAIRNARKLRELLAAPSRLR"
     gene            27961..29151
                     /locus_tag="VFC01_28980"
     CDS             27961..29151
                     /locus_tag="VFC01_28980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN83687.1"
                     /translation="MTTSSTFAIVGGGLAGAKAAEALRDKDFDGHIVLFAAEEHLPYE
                     RPPLSKEYLAGKKEFGDFTAASSAWYRDHHVELQLGTEVSAIDPGAHTVSLPDGSTVG
                     YDKLLLATGSHPRRPPIPGADADGVYYLRTVDDADALNSVLSEGSSLAVIGGGWIGLE
                     VAAGARDRGVNVTVVEMAELPLMAALGREVGEVFATLHREHGVDLRLGATVQEITTAG
                     GKATGLKLGDGSTVDADAVLVAVGAAPNTALAEQAGLALGDGGVLVDASLRTSDPDIF
                     AVGDIAAAEHPLFGTRIRTEHWANALKQPAVAAAGMLGKPAEYDELPYFFTDQYDLGM
                     EYVGHAPTYAKVVFRGDVAKREFTAFWLNADDRVLAGMNVNIWEGLDDIKSLIRSREP
                     LGAA"
     gene            29181..29714
                     /locus_tag="VFC01_28985"
     CDS             29181..29714
                     /locus_tag="VFC01_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83688.1"
                     /translation="MAILAAGCADEPIINTPEPPAQTGSAAPAAPSTPASNAHLANAF
                     DYVAHVGDTTGYYFTTPSGKWRCAILTHLKAGCQAASSWQSGLGVPDEPESTNSIVVD
                     RDGDPQFLALEQPEFWPNPGPAKTLPFNRILAAAGFRCNVQESGVSCQSAASGKGFTF
                     SAEGFTPQYTDVPADAP"
     gene            complement(29718..31079)
                     /gene="dctA"
                     /locus_tag="VFC01_28990"
     CDS             complement(29718..31079)
                     /gene="dctA"
                     /locus_tag="VFC01_28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HZN83689.1"
                     /translation="MPMTDVSVAPRKAWDSPLFVQLLVAIVAGIAVGWLWPAFGADLK
                     PLADGFIKLIKMLIAPIIFCTVVIGIAHVGDLKSVGRIGVKALIYFEVVTTFALLFGL
                     VIGNLVKPGAGFNIDPQTLATGAEAVAKKTGDGELPHTVEFILNIIPTSVFSAFAENM
                     LLQVLFFAVLFGLALAKFGEHGPPVVFELIEHLSSIFFTVIGWIMRLAPVAAFGAMAY
                     IIGQYGISSLGSYAKLIAACYLGAAFFILVLAAIAWFLAGVNLWQFVVYIKDELFLAL
                     GTASTEVVLPRIMVKLVDTGCSRTTTGLVVPTGYSFNLDGATLYLSICVLFLAQALGV
                     DLSLGEQVTAILVLMLTSKGMSGVPGSSFLALSATVAAIGHGAIPVAAVALLLGADRI
                     MDSMRVSVNLLGNCVATLVVARWEGQLDTDRMRKVLRGQTVEPLDEVAHAEDRKEPVA
                     TAS"
     gene            31157..31951
                     /locus_tag="VFC01_28995"
     CDS             31157..31951
                     /locus_tag="VFC01_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN83690.1"
                     /translation="MRIETGQVAVVTGGTSGIGFAIAEALVRRGVNVMIADIREDAIP
                     LAVDALAGQRGHAAGVRADVGVDADVDALADETLERFGRVDLVCNNAGVVCEQAPTWE
                     QRPATWRWLIDVKLMGVVNGVRTFAPLLIAQGSGHFLNTASAGGLMALPTLAPYAATM
                     HAVVGLTETLNAELRSVSTLLGATVLCPGLVDTALGANSVRLVPPGAATTPANAGGMP
                     AGAISPSVVAQAAIEAVEADRVHAIVGPATDVVVRQRVDGLLADLG"
     gene            complement(31948..33423)
                     /locus_tag="VFC01_29000"
     CDS             complement(31948..33423)
                     /locus_tag="VFC01_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HZN83691.1"
                     /translation="MPIDPRTPVLIGYGQVNQHDDHPGVEPVDLMVAAARAAADPRVL
                     EAVDSIRVVSVLSWRYRDPGLLLAQRLRAKDAATRYTGVGGNVPQTLVNQACLDIQTG
                     RADAVLIAGAETWRTRTRLRASGAKPDWTSQDETVPMAEGVDEGVPMAGPAELRIKLD
                     RPAFVYPMFEQALRIANGETAEEHLRRIGELWSQFSGVAAKNPHAWSREPLAPHQIWQ
                     PSPHNRMISWPYTKLMNSNNMVDQGAALVLTSVEKATYLQIPSDRWVFPYAGTDAHDT
                     YAIGERAEFYGSPAIRVAGQRAMQLAGVGVDDLDQIDVYSCFPSAVQVAANELGLPVG
                     DPNRPLTVTGGLTFAGGPWNNYVTHSIATLAERLVANPGQRGFITANGGYLTKHSFGV
                     YGAEPPSHEFRWEDVQSDVDREPTRKAVVEWSGVGTVEAWTTPFSRTGTAEKAVLAVR
                     TGDDARVLAVIPDPAEADVTIREDIAGVKVQVNSDGTATLL"
     gene            complement(33461..35755)
                     /locus_tag="VFC01_29005"
     CDS             complement(33461..35755)
                     /locus_tag="VFC01_29005"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN83692.1"
                     /translation="MSNGLLGQLRVLDLGGAESDAVGRLFADLGADVLKIESPAGSPA
                     RHAPPAVAGVSIPFALHNANKRSAVLDPDSAADRDRLVELAGTADIVVDGGGRAAAYG
                     TSCAALADRFGHLVALSVTDFGTSGPYASWQATDPVLYAMSTALSRTGPTTGTPVLPP
                     LGVASATAAVQAAWAALAAYYHRLRCGTGEYIDFSRFEAVVQSLDPPFGSEGQAAVGV
                     KRSYELWRGRPRNQQIYPIFPCRDGYVRICLLSPRQWRGMRAWLGEPERFADPKFDTI
                     AARYAASRELNEAIAELFAAGAMHDLVAEGQRRGVPIAAVLTPAQALSSEHFRAVGAL
                     TDVEAGVSVPVGPFVVDGEHVGYTTTAPSVGADEPTWATERSETAVVSGAAGRPFDGM
                     RILDLGVIVAGGELGRLFADLGAEVIKVESAAYPDGLRQTPPGQVMSRSWALTHRNEY
                     SLGLDLRHPAGAALFEKLVTSADAVFANFKPGTLAALGFSYERLRELNPRIVLAESSA
                     FGATGPWSARLGYGPLVRATTGVTWLWSADHASRNSFYDATTIFPDHVSARLTAIAAL
                     AAVVGRARTGTGAHVHISQAEAAVNQLAPAYVAEAARAANLPVADDTAVHGVYPCDGD
                     DEWCVISLRDDDDRAALSSVTGGTGVSEWTRTRDKAEVAHLLQRAGVPAAPMNRAVDV
                     LADPQITFRKLFSDMTHPLFGAPMPTETRPAPYRNIPPAELRPAPMPGEHTREICQKL
                     LAMNADEIDRLITDGVLFTHRGRP"
     gene            complement(35752..36636)
                     /locus_tag="VFC01_29010"
     CDS             complement(35752..36636)
                     /locus_tag="VFC01_29010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZN83693.1"
                     /translation="MLGFAVPQFGQSAHLDPGELARFASTAEQLGADSLWVGDRLLAA
                     VTPTVGYGGKDTIPEQFRANLDPFIALTVAATATTTARLGSSVFVAPWYPPAQLARQL
                     TSIDVISGGRLLPGFGIGWSPEEYQAAGAPFHRRGAQLDEILDALDALWTANPARHDG
                     ERWTIGESWVDLKPVQRPRPPVYLGAFTAPGLKRVGERADGWMAGVSVPDVVSLEMLN
                     WQRQTIDDAARAAGRDPSAIHTYVRVNVAEGTAVDKVADAVKLLADNGYPDAFIDLMY
                     VATGTDAHLDWVERLLTR"
     gene            36714..39095
                     /locus_tag="VFC01_29015"
     CDS             36714..39095
                     /locus_tag="VFC01_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HZN83694.1"
                     /translation="MSQMTTQALSPTLSDSELDLINAYWRAANYLSVGQIYLLDNPLL
                     REPLAPEHIKPRLLGHWGTTPGLNLIYAHLNRVIRHRDLNVIYITGPGHGGPGLVANA
                     YLEGTYSEVYSGIEEDVDGMRKLFRQFSFPGGVPSHVAAETPGSIHEGGELGYALVHA
                     YGAAFDNPDLVVACVIGDGEAETGPLAASWHSNKFLNPVTDGAVLPILHLNGYKIANP
                     TVLARIPQEELESLLYGYGYRPITVAGDDPANVHQQLATALDEAFDQIGAIQQAARLD
                     GETGRPLWPMIIMRTPKGWTGPKEVDGKKVEDTWRSHQVPLSETHSNPEHMAQLETWL
                     RSYRPEELFDEHGVFRPQLRALAPTGQRRMSANPHANGGLLLRDLDLPDFTEYAVAVD
                     NPAAGTAEATRVLGTFLRDVIARNPDRFRLMGPDETASNRLAAVFETTDKAWLAETEP
                     DDENLGPEGRVMEVLSEHLCQGWLEGYLLTGRHGLFNCYEAFVHIVDSMLNQHAKWLT
                     RNRELPWRQPIASLNYLLTSHVWRQDHNGASHQDPGFIDHVANKRPEVVRVYLPPDAN
                     TLLSVADHCLRSRDYVNVIVAGKQPALAYLDMESAIAHCTRGLGIWEWAGTATGEPDV
                     VLACAGDIPTLETLAAVDILRRELPELAVRVINVVDIMRLQPDSEHPHGLSDHEFDSL
                     FTTDKPVIFAYHGYPWLIHRLTYRRANHAQMHVRGFKERGTTTTPFDMVMLNDLDRFH
                     LVLDVIDRVPGLANRAAVLRQRMLDARLAARRYTREHGEDDPGISGWTWDWDR"
     gene            39107..39868
                     /locus_tag="VFC01_29020"
     CDS             39107..39868
                     /locus_tag="VFC01_29020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZN83695.1"
                     /translation="MSEQALSPHPHPVVGQLAALHHFRTYVDIGVVVVVLALTNLIAH
                     FTTPWASIATVPAAAVGLLILVRSRGLGWSELGLSREHCKAGAGYALAAVAVVGSVIA
                     IGLLLPWTRPMFMNNNYATVSGALIASMIIIPLQTVIPEELAFRGVLHGALNRAWGFR
                     GVAAAGSLLFGLWHIATSLGLTSSNVGFTRLFGGGLLGTVAGVVLAVAATAAAGFVFT
                     WLRRRSGSLIAPIALHWSLNGLGALAAALVWHLST"
     gene            complement(39865..40449)
                     /locus_tag="VFC01_29025"
     CDS             complement(39865..40449)
                     /locus_tag="VFC01_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HZN83696.1"
                     /translation="MTPAPVHWPGDDERKPAPGPLALVQSLVNTVELPDGADRLADPA
                     DARPWLADTGLLAPKAALTDADLEVVRAVREALRAMLVHNAGGPPPIKSALAVLRSVA
                     DGGTARASLADDGEVRLLAAGDSVRDRLVDLLLIVRDAQRDGTWKLLKACANDECQWA
                     FYDRSRNHGGTWCEMSVCGNKLKNRAFRARSKTR"
     gene            40562..41050
                     /locus_tag="VFC01_29030"
     CDS             40562..41050
                     /locus_tag="VFC01_29030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83697.1"
                     /translation="MTVPNNKISPDLLSTASDALRRTATPRPSLAARVTARLRAHQLD
                     RQLAVGVPAPAGSALAVHQARLVTVAEREAIARTLRQALDDANAGAAPLSSRIPLHPT
                     NIVAAEDLIDAITLRLHSPRPVSPLGMARLRVVLSDGSGPMYRFGRGDLSGRLGAALA
                     EL"
     gene            complement(41605..>42159)
                     /locus_tag="VFC01_29035"
     CDS             complement(41605..>42159)
                     /locus_tag="VFC01_29035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HZN83698.1"
                     /translation="LLCAGIIGYRSLLRADLPAGGRLGIYGFGGSAHLTAQVALAQGA
                     EVHVMTRGEQARELALTLGAASAQGATDRPPVALDAAILFAPVGDLVLPALEALDRGG
                     TLAIAGIYLSDIPALNYERHLFEERQVRSVTSNTRNDAREFLAFAGQHHIEVTTPEYP
                     LGRADDALSDLSEGRIAGAAVLMV"
BASE COUNT         7217 a        14021 c        13783 g         7138 t
ORIGIN      
        1 cactggccga ggcgaacccg agccaggtat gcctgttgcc agcccagcac cagccgacct
       61 tcggcgcgtc gcgattggcg ggcccgtcgc ggccggtgcc agtgctgatc cacacggcag
      121 gcgtcctggc gtccagcgaa ccggcggcct tacgtcggcg cgacccgatc gtcatgaagc
      181 aacgcccgcg ggcaagcaca tccggccgct gcaacagcca gtgcacaccc tcgctcaggg
      241 tcagcggcgt acggccggct tcgatgatgg ccggcaacgc ctcgtcgggg ctccagttgg
      301 ccatcgcgtc cccgcggtcg aggtcgcgaa gcaaatagag cggagcatcc gggagcttga
      361 ccgacgggat cggcgcgaac tcgtcgacat cgaccatgtc cgtcacgaca aaccccggct
      421 tgtcgttcac ccgcaacagc ggcgccaacc tcgacggtgg cgtgagatcg gggtgcacga
      481 ccagcagcgc atccgagctg tcgggaaggt ttcgtagccg atcggcgggc agccccgcca
      541 tgcggtcgac accgagctcg atcaggcgct cggcctgttc gcggacgctg gacacggggt
      601 ctcaacgacg cgccgcctgt cgatagttcc gacgaccagc acagctaaca caatcgacac
      661 accaccgacg cgcgaccgcc atcattctgt cgaaaattga ccggatggca gttaacgcgc
      721 atggaccgtc catcgggccg tccgtggccg agatcctggc gtcacacgcg ggcggtaccg
      781 gttcaccaac caagaccgcc gcccgtgtcg ccgatgcgat tgcggcccgc ggcgacgacg
      841 gcacctggtt gtcgatcgtg ccgcgcgatc aactgctggt caccgccgaa gagatcgaaa
      901 agcggcccgg cgcaaggaca ttgccgctct acggcgtgcc gttcgcagtc aaggacagca
      961 tcgacgtcga gggcgtgccg accacgctgt catgtcccga ctacgcctat gtttcgtcga
     1021 cgaccgcccc tgtggtgcgc cgtctgctcg acgccggtgc gctatacgtc ggcaagacaa
     1081 atctggacca gttcgccacc ggcctcaacg gcacccgcac gccgtacacc atcccgcgca
     1141 gtgtgtacgg cggcgacatg atctccggcg gctcgagttc gggttccgcg ctggccgttg
     1201 cgctcggcca agtgccgttc gcggtggcca ccgacaccgc cggctcggga cgagtgcccg
     1261 ccgcgctgaa cggggtcgtc gggttcaagc cgtcgcgcgg gctgatcagc acggtcgggc
     1321 tggtcccggc gtgcagatcg ctggactgca tcagcctgat ggccggggcg atcgacgatg
     1381 tcgatcgcgt cttcgacgtg gtggccgcgc gcgacgacga ggacgcgtgg tcgcgtgatc
     1441 gcgggccgcg ctacggcggc tcgtcgatcc gcgtgggatt gccgccgtcg gacgagttgg
     1501 acttcttcgg cgacgaggag atgcggcgtg ctcatttggc gtttcgcgag cagctggacc
     1561 gcaccgccac cgtcgtcgac gtctcgctga agccgttcct ggcggccgga gctctgctct
     1621 atcaggggcc gtgggtcgcc gagcggctgg tggagttcgg tgatttcctt gccgcccagc
     1681 cagactcgat ccaccccgtc gtcagggaga tccttcgcgg cggccagcgc tacacggccg
     1741 tcgacgcgtt cgccgcgtta cagcgcctcg ccgaactcaa ggcgctggtg gcgcggctct
     1801 ggcagcacat ggacgtgctt gtcgtgccga ccatcggcac cacgttcacc gtcgacgagg
     1861 tgcttgcagc gccgatcgaa accaacacga tgctcggcca ctacacgcat ttcggcaacc
     1921 tgctggacct gtcgggtgtg gcggttccgc tcggcatcac cagcgacggg cgtccttaca
     1981 gcgcaatgct tctgggtgcg gccctgaccg acgacaccgt gctgcatctc gccgcacagg
     2041 ttctcgacga accacgccag gtgcggccgt ccgccgtatc gaccatgacc gaggagcctt
     2101 atgagccaac ccatcgaagt gcccgccgaa ccgtcgccgt tccggctcgt cgcgggtaag
     2161 accgcgttga tcgtcatcga catgcagcga gacttcctgc tacccggcgg ctttggcgaa
     2221 agcctcggca acgacgtcga tcagttgctc aaggtcgtgc ctccgctggc cgatctgatc
     2281 gccgcggccc gctcggcggg gatcatggtg atccacaccc gcgaaggcca tgagcccgat
     2341 ctgtccgact gcccgccggc caagctcaac cgcggcgagc cgtcgaaacg cattggggat
     2401 gaaggcaagt acggtcgcat cctgatccgc ggtgagtacg gccacgacat cgtcgacgaa
     2461 ctcgcggcga tcgacggcga agtcgtcatc gacaaacccg gcaagggcgc gttctacgcc
     2521 accgaactgc aggacgtgct gaccgcggcg gggatcaccc agctactggt caccggcgtc
     2581 accaccgaag tgtgcgtgca caccaccacc cgtgaggcca acgaccgcgg ctacgaatgc
     2641 ctggtcgtat ccgattgcgt cggttcgtat ttccccgaat tccagcgcat cgggctggaa
     2701 atgatcaagg cccagggcgg catcttcgga tgggtcgcag acaccgccgc agtgataccc
     2761 gcattgcacg cattgacgac caccgccgcc tgagaggacc gcaccatgac caccgacgtc
     2821 acgaagccac ctgccgatcc gccggtagat ccgcccgccg tggccacgtc gcttccgctg
     2881 tggacccgcg gtgacaccaa cgcgttcttc ggactcggct tcaacatcct ggtcaacgtc
     2941 ttgacgttga ccggcttgat gatcggcgtc gtgtcggtgc ccgccggcga cgtgctgggc
     3001 actgtgctgc ccgcactcgg cgtggcgttg attctgggga atctgtacta cacgttcctg
     3061 gcccggcggc tcgcccgtcg cgagaaccgc gacgatgtca cggcactgcc gtacggcccc
     3121 agtgtgccgc acatgttcat cgtggtgttc gtcgtgatgc tgccggtgta cctcaacacg
     3181 aaggacccga ttcaggcgtg gtcagccggg ctggcatggg cgttcatgat cggcgtgatc
     3241 gtgatgatcg gtgccttcgt cgggccgtac atccgcaagc tgaccccgcg tgccgcgatg
     3301 ctcggaacgc tggcgggcat ttccatcacg ttcatctcga tgcgcccggc cgcgcagatg
     3361 tgggaggcgg cgtggatcgg ccttcctgtg atggccatca tcctgatcgg cttcttcacc
     3421 aacgtcaagc tgccgttcgg gatcccggtc ggtctggccg cgttgctcgt cggcaccgcg
     3481 atcggctgga tcggcgggtt catgtccgca cccgatgtgg gacaggcgat ctccgacatc
     3541 gccatcggta tcccggatct gcgcctcgat ctgctcttcg gtggcctgtc gaatctggcg
     3601 ccactgctgg gcaccgcaat cccgttgggt gtctacaact tcaccgaggc gatgagcaac
     3661 gtggagagtg ccgctgcagc gggcgacaac ttcaacctgc gcagcgtgct gctcgccgac
     3721 ggggcgggcg cggtcatcgg ctcggcgttc ggctcgccct tcccgcctgc ggtctacatc
     3781 ggccacccgg gatggaagga cgccggcggc cgcgccaact actcgcttgc cagcggtctg
     3841 atgatcggaa tcctctgttt ccttgggctt ttcggggtgc tcgatgccct actgccggtg
     3901 cccgcgatcg tgccgatcct gctctacatc ggtctgctga tcggcgcaca ggccttccag
     3961 gcggtgccgc ggctgcacgc cgtcgcggtg gtcgcggcgc tgctgccgaa cctcgcgcag
     4021 tgggccagcg ggctcattga caacgccttg aatgcggcgg gcacgtcggc cgcgaaggtg
     4081 ggcctggagg cgctcaacgg agctggtgtg gtctacgacg gacttcagac gctcggtgag
     4141 ggcgcggtgc tcgtcggcct gatcctcggc accatggtga cgttcatcct ggagaagaag
     4201 ttcctctacg ccggcattgc gtcgttcgtc ggcgcggtgt tgtcgttcat tgggctgatc
     4261 cacgcgcccg aggtggcgtg ggccgccacc cctcaagtcg cgctgggcta cgtgttcttc
     4321 ggaatcgtct gcgtcgctta ctcattcctg ccgggtgcga aggacccggt cgacgtcgat
     4381 gaatccgata tcgtcgccgg ccactgactg atatgtaggt cagtttgtca agggggcagg
     4441 gtgaagcggc ggccgcgacc gtcgtcgtgg ccgccgcttc gttgaccaga tgagtgtcgc
     4501 tgtgggtcga gcgtgtcgtg tcgacgcgtg tcagtctgaa atgcgcgggt tggttaccgc
     4561 aggacggacc ttcgaatcgg agttgatcgg cgggtctagg gtcgagcgtc atcggttgca
     4621 agacaacatg attgctcata gtcagatcgc gggtcactcc aggacgcttg ccgcccgtca
     4681 gcgtgtgttc atctggacac ttgtgggggt gggtcagtcc ggcatgactg ccagcaacca
     4741 gcaccagccg gccagttctc gggcgatcgc gacgttggcg atcaccgaac gcttcttgcg
     4801 ggcgtcgaag ccgacccagc gggtgtgcag acgccggttg gcggcctggc ctcgggctcg
     4861 ggcagccgga gaggccagat cccagcggcg ccgcagctca gctccgggcc ggtatgggct
     4921 gcggtggtgc caggcggctt cgaccaggag gcggcgggcg tgtccgttgc cggtcttggt
     4981 caccccaccc tgggcgcggg agtctcctga ggagtgctcg ctgggcacca acccgacata
     5041 ggcgccgatg gagcggccgg ttaatcggtg ccagtcgccg aattccacgg cgagcccgaa
     5101 ggccgtcaac gtcgcgatgc cgcggataca acccaaccgg tgcacgaccg gggtgaactc
     5161 actatcggcg gccatcgcgg tgatcgcgtc atcgagacga tcacgtcgat ccagacaggc
     5221 cagcatcgca tcgaaggctg cctcgtagct cagttgcagt gctgggttgt cgaaccgctg
     5281 ggcgcgtagc cataactcgt gccgactggt ccatgcctta ccgccgaagt agacaattcc
     5341 ctggcgcagc aacagtttcg agacccgatg ccgtgcggtc atcagatccc cacggcagtc
     5401 ctcgcgggca cggacgagat cacgggctgc ttcctgggcg gcggtgggaa ccgtcactgc
     5461 ggtgatctga cccaggtgca aaagccgcgc cagatgcgcg gcatcacggg tatcggtttt
     5521 gacccggtcg ccagccgggc ggatcaactt cgacggtgcg gccacctcgc agctgattcc
     5581 ggctgcggtc aaggctcggg ccaatccgaa cccggtcggg ccggcttcat aggtcaccgc
     5641 taccggacta gggagatcac ccagccaggc cacgatctcc ccgtgatccg gggtcaatcg
     5701 tcgttggaac acctcaccgg tgtccccgtc caacccacat ccgaggaccg aacgtgcgtg
     5761 cacatccaac ccgacactga accttcactg gggcctccca catctgtggc tctaccggcc
     5821 aggacccaat tcctgacggc aacccacgtt cacatgtgag cgaggcccca gcccccatag
     5881 cgtctagtcg tacggcgtga tcagttcagg cggccccgtc ggcaatcgcc aatggaacgc
     5941 gagccctgcc agcctgagcg cagtcgccac tgcagagccg accgcaatgg ctatccattg
     6001 cggccagaaa tagtcgatcg cgacgtaggt cgccgcgccc agcatggccg gtatcgcgta
     6061 caggtcgtca gggcccatca ccaggggcac ctcgcgcgcg atcacatcgc gcaacatccc
     6121 gcccgcgatg gccgtgagca tgcctatcag gccggccgcg aaccaactcg caccttcgtc
     6181 gacggcgatc gcggcgccgg aggtggcgaa gaaccccatc ccgatggcgt cgaggacgag
     6241 caccaccgtg ttcatccgga tcacgatgtg ggcgaacgga gtggcgatgg cgactgcgat
     6301 gagcgcgact gtgatgttgg gccagtgctg gatcgaatac ggaggcctgt cgagcagcag
     6361 atcccgcagt atgccgccgc cgactccggt caggactccc agcgtcgcga ttccgaacac
     6421 atcgaagcct ttacgcaccc ccaccaccgc acccgaggac gcgaacaccg cgatgcccgc
     6481 atagttgaga attgtctgca gcacgcaaac acgttaatcc gcgacaaacg gcacgtcggc
     6541 gccattggcg agcacgacgt acatccggcg ccacgggtcg gaaccctgca gccgccactg
     6601 gtgtcctgaa cccgcggtgt cctcggcaag cagcacgtct cccggccgca gcgtgaactc
     6661 ctcgccgtcg cgggtcgaga agatcagcgt gccggccagc gtgacgacga gttggcgcac
     6721 cggcgcggtg tgccatgcca gcgatccccg gtggccgtct cctcgacggt tacgtgcgat
     6781 gccgaaatcg cggtcgacgc cagatcggcg ttgcggccgg gtgcgatatc gagacggccg
     6841 acctgaacgt gtgatgcctc atccgaacct gtacaaagcc tgacgcatcg aatcatttcc
     6901 gactccatgt cgcacgcggg ctcttacgct gctgagtgat gaacaccgcc accgtagtcg
     6961 tgacgatcct gcttgcggcg ctcttcacgt tctccgccag catcaaattg ctcggcgtcc
     7021 gccaatccct ggccatccgt gaccgcctcg gcgtcaagcc gctgcagtgg cggctcatcg
     7081 gcgtgtgcga actcgccggc gtcgcaggag tgctggtcgg tctgctgtgg acgccgatcg
     7141 gcatcgccgc cgcgatcggc ctcgcgctgc tgacgatcgg tgccatcgcc taccacgtac
     7201 gggtgtccga cacggtcgcc gatctcgcgc cggccgtgat cggcgtccta ctggctctca
     7261 cggccgtcgt actgcacacg ctctgacgta gcgggcgtca cacctgctca cacgggtggc
     7321 ttagattacc taaattttcg tggtcagttg tctacgaagt ggcttacgct gcttgtatgg
     7381 cagcacgagc ccacagcgtc gatccccgcg ccgaacgggt ccgtacccgg cttcgggagg
     7441 cggcgttcgc gctggcgcac gagggcccgg tcgatgagct cacggttggc gacgtcgtcg
     7501 cccgtgccga ggtcagccgg caggtcttct atcaacactt ccgcgatcgc gatgacgccg
     7561 tcgccaccgc attcaccgtc gcgttcgccg cggcgacggc ggacatcgac accgacgccc
     7621 gggcccggat cttgcggctg ttcgacttcg ccgcggagca ccggtccatg taccgcaaca
     7681 tcgtgccgag cgcggtgacg cagcgcgtcg tcacggcgtt ccgagccgaa ctgcagccgg
     7741 cgtgtgtcca gatcgcgacg caggggatgc cggtggtctc cccgatcgcg agcctgaccc
     7801 ccgattcggt cagccggttt ctggtcggcg gattcatgga ggtgctgcgg tcctggatgg
     7861 aagacccgga ctcgaccgac ctcggcgccc gcgtcagcgc ggcgctcgac accgtcaacg
     7921 cactactcgg actcaccaca tcttcgaaag gtcaacaaca tggctaagca ccaagccccc
     7981 gagcggcacc ctgtgcgcga tcgggccgcg gttcgcacca cggcgctcgc gatgatcgga
     8041 ttgacgctgt tcgtcatcgc catgatcgcc agctactccg gtgccttcgc caagccgaca
     8101 ctgcaccaca tggccgtggc cgtggctggc ccgcaacagg tcgtcgacgg catccgcggc
     8161 caggaatcgt tggcagtcac cgaagttggt gatgacgccg ccgcccgtca acaggtctac
     8221 gagcgcaaga ccgacgccgc gttcgtcgtc gccccgaccg gcgacatgaa gatctacgtt
     8281 gccggtggcg gcgggcgcag tgtggccaac gccgccgaga ccgtcggccg caccatcgcc
     8341 gccaaaacag gcctgaagcc ctcggtcgag gatgtcgccc ctgcgtcggc aggtgatccg
     8401 tccggcacca tcgagttcta cgcgatcatc ttcatttcga tcggcgcgtc cgtcggcgca
     8461 accgtgctgg gccgtctcat gggcgccgtc cgcaggccgc tgacgctggc cctgcgcacc
     8521 gccacgctgg ccggattcgc ggcggtgctg gccggcgcgg tgaccgttta cgtcgacatg
     8581 attcttggcg cgctgacggc tcacacctgg catctgttcg gggcgctatg gctgtatgcg
     8641 atggcagtcg cgggggcgat caccggtgtg gcggccgcgt tcggctcggt cgcatcgatg
     8701 gccctgacgg ccttcctggt cgtgatcggc aacgccgcgg cagctggtcc cgtcggccgt
     8761 cccctgctgt cgggcttcta ctcgacgttc accacgatcg tgccgcaggg gtcgggcgtc
     8821 tcactgctgc gcagcgtcga atacttcggc ggcaacggtg ctcagacgcc gcttgtgacg
     8881 ctggcgatct ggggtgcggc ggggtgtctg ctcgcaatca tcgccactgc ttcgcgcgtg
     8941 aattatcgtg ctgtctatga gcgtttcgct ggccgaaccc tattacgacc tcgggtccta
     9001 tcaccggccg attgagacgg cgtcacatga ggcgcagctg tggttcgacc gcggcatggt
     9061 gtgggcgtac gcgttcaatc acgaagaggc gatccgctgc ttcgatcgcg cgctggaact
     9121 cgaccccgac ctggcgatcg cacgctgggg cgtcgcctac tcgatcggtc cgaattacaa
     9181 caaggcatgg gaggcgttcg atcccgcgga cctggccgcg tcgttggcgc gtgcgcggat
     9241 ggaactcggg ctggccgcta agggtcgcgc atcggccgtc gaacacggtc tgatcgaggc
     9301 gctgcaagcc cgcttcccca ccgacgaccc caacgacccc gacgcgctgc aggccgggca
     9361 caccgcctac gccgacgcca tggtcgccct cgccgaggcg taccccgacg acatcgacgt
     9421 gcaagcgctg accgccgacg ccctggtcaa catcacggca tgggcgttgt gggacatcag
     9481 aaccggtgaa ccggcacccg attcgcgggt ggtcgaagcc aaacggatcc tggacggcat
     9541 gttggccacg cccgaaggac gcaagcaccc tggtgtgctg catctgtatc tgcacacgat
     9601 ggagatgtca ggcactccgc aagatgcgct gcccgccgcg gaccttctgc gcgggctggt
     9661 ccccgacgcc ggccatctgg agcacatgcc cagccatatc gacgtgctgt gcggcaacta
     9721 ccgcgactcg gtggtggcga atcagtctgc ggtgcaggct gaccggcgat tcgtggaacg
     9781 cgaaggaccg ttgcacttct actcgctgta ccgtgctcac aatctacact tcatcgtcta
     9841 ttcggcgatg ttcgaaggga attcgcgggc cgcgttcgac gccgccgaag aactggcggg
     9901 tcagttgacg cccgagctct tggcgatcga gtcgccgccg atggcggact ggctggaagc
     9961 gttcgtgccg ttgaagattc acgtgctggt gcgattcggc cggtgggatg agttgattgc
    10021 cctaccgttg cccgacgacg tcgagctcta cagctgtacg gccacgacca tccactacgg
    10081 acgcggtgtc gcccacgccg ccaaaggtca actaccgcag gcgcatgcag aacgcgacgc
    10141 gtttgcggag gcttatgccc gcatacagga cacccgctac ctgttcaaca acaccgcccg
    10201 cgacatcctc gccatcgccg cagagatgct cgacggtgaa atcgactatc gcgaagggag
    10261 attcgaggac gcgtttgccc acctgcgtcg cgccatcgaa ctcgacgaca acctgcccta
    10321 cgacgaaccg tggggctgga tgcagccgac acggcacgcc tatggcgctc tgctgctcga
    10381 gcagggtcgc gtcgaggagg ccgcgcaggt gtatgccgcc gacctggggc tggacccgac
    10441 gctggcccgg ccctgccagc atccgggcaa cgtatggagc ctgcacggct accacgaatg
    10501 cctgcagaag ctgggacgca ccgacgaagc tgcgctgatc ggccagcagc tcgaactgct
    10561 gaaggcgcgc gcggacgtcg cgatcactgc gtcatgcgcg tgccggctcg aggtcgccga
    10621 gccctgtcac tgaccgctag gcaggcgctt cgtgcagctc gatcgaattg ccgtcgggat
    10681 cggtgatctg gacctgcttg ccgccggggc cttccctgac gtcgttgagg aacgtcacac
    10741 cggcgttgcg cagacggtcg acgtccgccg caatgtcgtc gacgtagagc acgatccggt
    10801 tccagccgcc gggctgctgg gtttggccgt ccgctagtgg ctgcgcgccc gagctcgccg
    10861 gcgcgctcaa cagcaggttc aacgccccgc gcgcgacgac agcgatgggc ccggcgcgct
    10921 gcgcgagccg gaacccgagc tgattcgtat agaactcgat tccgcgatcg aggtcggcga
    10981 tctggtagcg cacgacggca tcgtctgtgc tcgtcgggcg ccaaatcccg gtagacatat
    11041 ccacagacta tgggctggcc tggtcagcgt cttggaggaa tttgacctcg tccggcacgg
    11101 ccaggtgtag ctccttgacg ctctcgctcg ccggacgcag ccggctcggt gacgtcccgg
    11161 tgaactcact gacctcgttg atgagatgcg attgatcgaa gtagccacag gcccgggcaa
    11221 gccgtgccca gtccggtgca ttgatgcgac gagcgagcgc actcgcacgc tggaaccgca
    11281 acactcgact caaccgcttt ggcgtcatgc cgacctcgct cgtgaacacc tgaatgaacc
    11341 tgcgtcgact gaggtgaaca tcctctgcga ccgcgccgac ggtgacgccc ggccgggcca
    11401 gccgcccgag tgcgaagggc accgccgggt ggccgggttg agcttcggcc aaacatgatc
    11461 gcaacgtttg ttcaagcagc gcgaatcgct gctgcgacgt cggcgccgca cacaaccgct
    11521 cacgcaactc gaccgccgag ctcccccata gcatcgcgag atcgatatgc cggtcggtta
    11581 gttcgccggg cggcacaccg agaaacggcc acgctccgcc cggtttgaag tgcacaccga
    11641 cgatcgacgc gtggtcgcgg gtgtcgatca cgaagtactg ccgataggca cccgacacca
    11701 ctgcgccgga atactcccgc cacaccccgg agccggggtc atatatgcgc agtgcgtcct
    11761 cgttgaggtt gatcaccagt tcgagggtgc cgctcgggac gattcgctcg tgcgcgtgaa
    11821 ccgccaggtc acgcatcgtc cacatgtacg ccacaaagtc cgccaacggc ggcgcgggac
    11881 gatacgtgcg gtactgcacg ccttgagggt tccccctcag ccgaaagcga ctcgctccaa
    11941 ccagaaatcg acgagcgtcg cgtcgccggc gatttccaga ccggcgaccg gtcggcggcg
    12001 gtagatcgcc aacaacaggt ctgtgaccgg tgcgcggatc tcggccgtcg ccggttcgtc
    12061 ggcgccccgc cacgcgatga catcgccggt caggtcgagc agccagtgtg cgtccgtgtc
    12121 ggtggcatgc agcccgatcg tgcggcccgg tgcgagaagc tcacgcatcc acggatgaac
    12181 ctcgaaatga aaaggcagac aacccaaatc catccactcg tcgacagcgt ccgccgcgac
    12241 gtctggttcg agcgtgtagt cgacgcctaa cgcaagcgct gcgtcggcgc ggtgcagcgc
    12301 ggtctcgtgg gcgaaccggc gggcataaaa cgccgaaccg ccgtcgccga cggggcacca
    12361 catctgtgca tccggccccg cctcggtcag ggtcgccctt agcgccgtcg cggcctgcgt
    12421 aagtgaagcc gccaggacgg caggatcgtc gttcgtggcg cccgagagat cgcgcagcgc
    12481 cgcatcactc ggcggctcgg atgcgcgcgt cgcgacgatg gcatctgccc agcgttgacc
    12541 gccgtcgacg tggcgcgcca actgcgacac attccatccc gggcacgtcg gcaccggcac
    12601 ggtcacgtcc gcgccgttca gatggtcggc caacaacgct gtctgcgtga cgatctcggc
    12661 gcaatgccgg tcgaatccga gcctcatgcg ggcggcccgc cgagccagat gtgaaaggtg
    12721 tccagccggc tcaccagacc atcggcgaac gtgaagacct ccgaatagac gaacgcatgc
    12781 ggatcgccgc ccaacgagac actgccgtgc ccgatcaccg cgaccgcgtc gtcctcctcc
    12841 atcagccggt cgagcgtcag ctccggattg gggccgtcgc cgctgtcggc ctctgccgca
    12901 aacgcgtcct tgccgaccag cgtcttggcg ccgtgcagcg cccacaccac gtcgtcggtc
    12961 aagcacgaca agatctgcgc gaggtcaccc ctgcggaatc cgtcggtgta cttctcgacc
    13021 actgccttct gctgtgacac atcatcacct ccaatgggtc gactatggcc gagtgccgga
    13081 ctcatcgcag atggccgaac ctctatcgtc accacgcgtg acgatggttt ggcactacat
    13141 caagactgca ccaagtggcg ttggctgctc gtggggctcg catgtcatgt cggggccacg
    13201 tctctcagcg aggggggccg gcgtgctatc cgctcacccg gtcgcggcgg cggtagcccg
    13261 cggctgcgta aactccggcc tgtggcgatc agcgacgacg acctcgaccg cctgcgccgt
    13321 tgtgtggaac tcgcacgcgt cgcactcgac gacggcgacg aacctttcgg ctcgatcctg
    13381 gtcgacggcg acggcgccac gctctacgag gaccgcaacc gggtcaagga cggcgaccaa
    13441 acccggcacc ccgaattcgc gatcgcccgc tgggccgtcg acaacctgac tcccgatgag
    13501 cgcgcccgcg cgaccgtgta cacctccggt gagcactgcc cgatgtgcgc ggccgggcac
    13561 gcgtgggtgg gccttggccg aatcgtctac gcgacctcgt cagaacagct gacgcaatgg
    13621 ctcgccgaat ggggcgcacc gccgccaccg gtggctccgc tgcccatcgc gacggtggcc
    13681 ctgcgcgtcg acgtcgacgg acccgcaccc gagctcgccg acaccatgaa ggccctatac
    13741 gaggcgaagt tccgaccatg agtgaaccgg gctggatcga gcacgcgatc tggtggcagg
    13801 tctatccgct gggcttcgtc ggcgcatatc ccgccgatcc cccgccgacc cccgatgagc
    13861 atcggctgcg ccggatcgtc gactggctcg accacgccat cgaactgggc acgtccggaa
    13921 tcgcgcttgg ccccgtgttc gcgtcgcgca cacacggcta cgacaccacc gaccattaca
    13981 gcatcgaccc gcgtctcggc gacgacgccg actacgacca tctgatagcc gaggcccatc
    14041 ggcgcgggct gcgggtggtg ctcgacggcg tgttcaacca cgtcgccggg gatttcgctc
    14101 ggccagagtg gttccgcaag cacggcaaag actttgacgt attcgaagga cacggcgagc
    14161 tgatcgcact cgaccacgac aacccggacg tcgtcgccta taccgtcgac gtgatgacgc
    14221 actggctgcg gcgcggcgca gacggctggc gcctcgacgc cgcctacgcc gtccccgaca
    14281 gattctgggc acaggtactg cctcgggttc ggcaagagtt tggcgacgcg tggttcgttg
    14341 gcgaggtcat ccacggcgac tacatagcgc gggtacgcga gtcgggcttc gattccgtca
    14401 cgcaatacga gctgtggaag gcgatctgga gcagtctcaa cgacggcaac ttccacgagc
    14461 tcgactgggc gttgttgcgg cacaacgagt ttctcgacga cttcgtgccg atgacgttcg
    14521 tcggcaacca cgacgtcacc cggatcgcca gccagctggc caacaccagc cacctcgagc
    14581 atgcgctggt aatcctgttc acgacgggtg gcaccccgag tgtgtacgcc ggcgacgagt
    14641 cggcctaccg cggcgtcaag gaggaacggt tcggcggcga cgatgccgta cggcccgagt
    14701 tcaccgctcc cctaatgggt gtcgacgatc tcggtcacga tgtgttccgg ttgcaccagt
    14761 acctgatcgg tttgcgccga cgccacccgt ggctgcacac agccaggaca tcaccgctgc
    14821 ggctgacgaa cacccagtat gtctatgaga cccgtaatag cgacgacgcg ctgctggtag
    14881 cgctcaacat cgacgaagca ccgttgccgc tttcgttgcc cgaactcggc ttcggccagg
    14941 gcgagatcgt cgcgggatcg ggcgcgccgc cacagtccgt cgtcgcggat gctgagatcg
    15001 cgccgcacgg ctggctgatc atcgcgccgc gctgagcagg tcgacggtag cgctgtcctc
    15061 tgcaccgtcg tagaacttgt cgagcacggc acggaagtcg gcctggacat cggggtcgtc
    15121 ggtcgcccgc gcgaacagcg tcatcgacga ccgcaccttg aggttgtcgg gccagccgaa
    15181 gacgtcgtcg gccgaagagc cgtcgattgc cgccaccagc cgggtgcatt cgcgcaaccg
    15241 cggtcccagc gtgtcatgcg ccaggtaggc ctgtgcctcc tctagagagg aaatgccgta
    15301 acggagtgcc gtctggctgc ggcccaatcc ccgcagctgc ggaaacacaa accaaatcca
    15361 atgacttcgc ttgcggccgc cgcgtagttc gtcgagcacc gtcgggtaaa cgcggtcctg
    15421 cgcgtcgaca aagcgctgca ggtcgaacgg atctgcagct ttggatgaca acgatttgcc
    15481 cacagtcaca tcgttagcaa gttttgcaag ctagtctttg cgattcaatg acgacaacgg
    15541 acgagcagga attatcgagc cagccggagc ctgcgcccaa gcccagaagt cttcggcgtc
    15601 gcgtgctgtc gcgggtcagc atccaatcca agctcttggt gatgctgctg gtgacgagca
    15661 ttctgtcggc cgccgtcgtc ggcgccatcg gttaccagtc ggggcgcagt tcgctgcgcg
    15721 cgtcggtgtt cgatgcgttg accgagattc ggcaatcgca aacccgccag ctgcaggctc
    15781 agctcacgga tttgcagaac tcactggtcg tcttcacccg cggatcgacc gcgatcgagg
    15841 cgactgaggc gtttaccgct gcattcgatc agctcaacaa cgccaccatt actccggcgc
    15901 aacagcagtc gatcgttgac tactacacga aggagttcgc tccggccgag gacgagcaga
    15961 ccggcgacga cgtcgatatc gatgcattgt tgccgacgac gaacgctcag aaatacctgc
    16021 aggccaacta cacgaccccg ttcaccgact tcgatcaagc gctcagtttc gacgacgcgc
    16081 acgacggcag cgcatggtcg gcggccagcg caaaattcaa cgatttcttc cgcgtaatcg
    16141 tcaagcgctt cgagttcgag gacgcgctgc tgctggacac gcgcggcaat gtcgtctaca
    16201 gcgcctacaa gggcgtcgat ctcggcacga acattctcac cggcccatac aaggctggcg
    16261 atctcaccga cgcgtacaac aaggcgctgg actcacacac cgtcgactac gtcgccgtca
    16321 ccgatttcag cgattaccag ccggccgacg aaccgacggc ctggatggtg tcccccgtcg
    16381 gcccgcagaa tcgcgtcgaa ggcgtactgg cccttcagtt cccgatctcg aagatcaacc
    16441 ggctgatgac gatggacaag cggtgggaag agtccgggat gggcaagacc ggcgaaacct
    16501 tcatcgtcgg ccctgacgac ctgatgcgct cggactcccg gctgttcctg gaggacccgg
    16561 aagcgttcaa gcgtgatgtg gtcaacgccg gtactccgcc cgatgtggcc gaggaatcga
    16621 tccggcagca cggcacgacg ctggtgcagc cggttgccac cgaagccacc cagcgcgcgc
    16681 agcgaggaca gaaaggcacc ctcgtcgcag acgattacct cgggcatgag acattgcagg
    16741 cctacgcgcc ggtcgacgtg tcgggtctgc attggtcggt catcgccaag atcgacacct
    16801 ccgaagcctt cgctccggta tcggcgttca cgcgcaccct ggtgctctcc acggtggtca
    16861 tcattttcat cgtctgtttg gccgcgatgc tgctcgcacg cctcttcgtg cggccgatcc
    16921 ggcggctgga agccggggct cagcgcatca gcagcggcga ttacgacgtc gcggttcccg
    16981 tgcaatcgcg cgacgaattc ggcgatctca ctgtggcttt caacgacatg agccgaaatc
    17041 tggcgatcaa ggaggagttg ctcaccgaac agcgcaggga gaacgaccgg ctgttgctgt
    17101 cgttgatgcc cgaaccggtg gtgcagcgat accgcgaggg cgaggaaacc attgcgcagg
    17161 atcaccagaa cgtcacggtg atcttcgccg atatcgtcgg actcgacgag ttgtcggccg
    17221 aactgacctc cgatgagttg ctgaccatcg tgaacaagct gacacgccaa ttcggcgccg
    17281 ccgcagagaa tctcggtgtc gaacaggtac ggatcctgca caacggatat ctggccagct
    17341 gtgggatgag cgtgccgcgg ctggacaatg tgcggcgcac cgtcgacttc gccatcgaaa
    17401 tgcagcgcat catcgaccga ttcaacaagg agaccggcaa cgacctgacc ctgcgggcag
    17461 gcatcgacac cggcactgta acgagcggcc tggtcggcag gtccacgctg gcctacgaca
    17521 tgtgggggtc ggcggtcaat ctcgcatatc aggcgcagag cggggcgccc caacccggcg
    17581 tgtacgtcac gtcaaaggtg tacgaggcga tgcgcgacag ccgtgacttc acgtccgccg
    17641 gtgtgatcac cgtcgacggt gaagagcagc cgatctggcg gctgggacag tcatgaggag
    17701 cgtgctcgac gcgccgtggt tctactgggc cgtcggcgta gcgatcggct ttccgatcct
    17761 gctggtgttg ctgaccgagc tgcaacacgc actgcgtcgg cgcggcagca tcctgactcg
    17821 gcccgtttac ctgctccgca cctacatcct gccgctcggt gcattgctcc tcctgctggt
    17881 gcaggcgatg cagatctccg gcgaggagac tccggttcgc gtggtggcaa cggtgttcgg
    17941 cttcgtcgtg ctggtgctgt tgctgtcggg actgaacgcg acggtgttcc aaggcgcccc
    18001 ggaagggtcg tggcgcaagc gaattccgtc gattttcctc gacgtcgctc gcttcgcgct
    18061 gatcgcgatc ggcgtcggtc tcatcttcgc ctatatctgg ggcgcgcata tcggcgggct
    18121 gttcaccgcg ttgggcgtgt cgtccatcgt gctgggtctg acgctccaga actccgttgg
    18181 gcagatcatt tccggcctac ttgtcctgtt cgagcagccg tttcaactcg gtgactggat
    18241 ccaaacaccg acagcacgcg gccgcgtggt cgaagtgaac tggcgcgcaa cgcatatcga
    18301 caccggacgc ggtttgttga tcatgcccaa ctcggtgctc gcggccgcct ccttcaccaa
    18361 tttcagcagg ccgcccgggg cccactcgct cagcgtgaca acgacgttcg cagtcgacga
    18421 cccgcccgat gacgtcatcg cgatgctgaa tcgggtggcc gcgatggtgc cacagctgcg
    18481 gcccgatgcc gcgccttcca gcgtccactc cggcggcctg cagtacagca cagggattcc
    18541 gctgcgctca cccgccgacg acttcgcgac gaagtcgatg ttcttacgct gggtttggta
    18601 tgcctcgcgg cgggctgggc tgcacctcga cgaggccgaa gacgagttct ccactcccga
    18661 gcggctaaag gcctcgctgc gcatcatcgc tcccacgctg cggctgaacc aggccgagca
    18721 gcagacactg ctcccgcacg cccggatcac caggtacgga tccgacgagt accttcagtt
    18781 cgccggtcag gtgcccaaac ggatgagttt cgtcgtcaat gggcgagtcc ggctcgtcgt
    18841 gaccggtgac gacggcgccg tgctcccggt gcgtaccctc gaagagggcg acttcattgg
    18901 gcagaccgca cttacccgcg agcccgtcgc cgcggcagcc tatgcgctcg aagaggtgac
    18961 ggtgttgcag atcgaacggg agtacatcga ggaactggtc gcgcgaaagc cgctgctgct
    19021 gcaggatatc ggccgcgcga tcgaggagcg gcgggccaac gtccggcgag tgctggccgc
    19081 cgccggcgag taggccgcga tgaatcgacg cgtgccccgg cccagcgaac tggcgcccct
    19141 gatcggcttc aagaagccac agctgaatgc gacgaagcgt aggcttgcgt ccgcgctgac
    19201 catcgacgac ctacgccgaa ttgccaagcg ccgcacgccg aaagccgcat tcgactacac
    19261 cgatggtgcc gccgaggatg aactgtcgct ggcccgcgcc cggcaggcgt tccgcgacat
    19321 cgagtttcat ccgacgatcc tgcgcaacgt cgagaaggtc gataccagcc ggaccgtgct
    19381 cggagacagg gtggcgctcc ccttcgggat agcgccgacg ggcttcactc ggctcatgca
    19441 gaccgagggc gaaatcgcgg gcgcccatgc ggcggcccga gccggcatcc cgttctctct
    19501 gtcgacgctg ggcaccagct cgatcgaaga tgtcaagaca gcaaacccgc acggccgcaa
    19561 ctggtttcag ctctacatgt ggaaggaccg tgaccgttcg atggcgctcg tggagcgcgc
    19621 ggccgcagcg ggtttcgaca cgctgctcgt caccgtcgac gttccggtgg ccggcgcgcg
    19681 gctgcgcgac acccgcaacg gcatgtcgat cccgccgacg ctgacgttgc gcacggtgct
    19741 cgacgcggtg ccgcacccgc ggtggtggtt cgacctgctg accaccgagc cactgtcgtt
    19801 cgcgtcgctg gatcgctggc cggggacggt cgccgaatac ctggacacca tgttcgaccc
    19861 gaccgtgacg ttcgaggacc tggcgtggat caagacgcaa tggccgaaca agttggtggt
    19921 caagggaatc cagaccttgg aagacgccag ggccgtcgtc gatctcggtg tcgacggtgt
    19981 gctgctgtca aaccacggcg gccgtcagct cgaccgtgcg ccggtgccgt ttcacctgtt
    20041 gccgaccgtc gcccgcgagc tcggcgagtc gacggagatt ttgctggaca ccgggatcat
    20101 gtcaggagcc gacatcgtgg ccgcgatagc gctcggtgcc cggttcacgc tggtcggccg
    20161 cgcctatctg tacgggctca tggccggcgg cgaagcgggc gtcgaccgcg ccatcacgat
    20221 cctgtctgag caggtcacac ggacaatgcg gctgctcggc gtgacctcgc tggacgaatt
    20281 ggcgccacac cacgtgacac agctgtatcg gctcgggcgc gccgtgccgc gtgcaggcgg
    20341 ctagtcacat ctgtaactct attcccacag gtcacggcgc ataatcggct gcgatgagtc
    20401 cgcgccgagt cggaatcgtt agccaaccgc gacgtcacaa ccctgtgttg agccaccgat
    20461 cgcctccttc ggacgtgttt cattacgccc gaagcaacgg caattgcttt gtggcaagtg
    20521 atgaaaggtg ggttggttgc cgttatgaag ttcatggaga agtttcgagg taagtggttg
    20581 cgccggctgg cggtgggcgc cgttgcagcg gcgaccctgc ccgggctgat cggcttcgtc
    20641 gggggttcgg cgactgccag tgcgttctcc cggccgggtc tgcccgtcga atatctggac
    20701 gtgttctcgg ccgcgatgaa ccgaaacatc cgggttcagt tccagggcgg cggaccgcac
    20761 gcggtctatc tgctcgacgg cctgcgcgct caggacgact acaacggctg ggacatcaac
    20821 accccggcgt tcgagtggta caacgggtcc ggcctgtcgg tcgtgatgcc ggtgggcggg
    20881 atgtccagct tctacaccga ctggtatcaa ccgtcgcggg gcaacggtca ggactacacc
    20941 tacaagtggg agaccttcct gacccaggaa cttcccgcgt ggctggaagc caacaagggc
    21001 gtgtcgcaga acggcaacgc ggtggtcggc ctctcgatgg ccggcagcac ggcgctgacg
    21061 tattcgatct actacccgca gaagttcatc tacgccgcat cactgtcggg cttcctgaac
    21121 ccgtcggaag gctggtggcc gatgctgatc gggctggcga tgaacgactc cggcggctac
    21181 aacgccgaga gcatgtgggg tccgtcgtct gacccggcgt ggaagcgcaa cgacccgatg
    21241 gtcaacatcg ctcagctggt cgccaacaac acccggatct ggatctactg cggcaccggc
    21301 accccgtcgg atctcgactc gggaacctcg ggcgaaaacc tgatggccgc gcagttcctc
    21361 gaggggttca cgttgcggac caacgtcacc ttccgggaca actacatcgc ggccggcggc
    21421 accaatgggg tgttcaattt cccgtcgcag ggcacgcaca cctgggccta ctgggggcag
    21481 caggtgcagc agatgatgcc ggatatccag cgcgtgctgg gggcccaggc ctccgcctaa
    21541 gtaccaccca caaccaggga gcctgccgtc acccccgaac ggcaggctcc ctgctttctg
    21601 cgcacagcga agcgggaaca aacacggtcc gcccagggtt gagtcgatct gactgaactt
    21661 tggaggattc atggctgaag ccaaatcagt gccagtgctg tttttgagcg actcgatcgt
    21721 gctgccgggc atggtggtgc cgatcgagct cgacgacgcg gcacgcgccg ccgtcgacgc
    21781 cgcacgggcc agtgagtccg gcgagctgct catcgcgccg cgtctcgagg accgctaccc
    21841 ctcctacggc gtgctggcct cgatcgtgca ggtcggccgc gtcgccggta gcagtggcac
    21901 ggccgcggtg gtgcgcggca acggccgcgc ccagatcggt gccggcgcca atggccccgg
    21961 acccgcgctg tgggtcgagg tgaccgaggt cgaagaggcg gaagcgaccg ccgagaccac
    22021 ggcgatggcc gccgaataca agaagctgct gctggcctac ctgcagcggc gtgaggcctg
    22081 gcagatcgtc gacttcgtga accagctgac cgatccgtcg gcgctggccg atatggccgg
    22141 gtacgcgtcg tacctgaccc aggtgcagaa gcgccagctg ctggagacgc ccgacgtggc
    22201 cgagcgcttg cgcgtgctga tcgactggac cagcgaccac ctcgccgagg tggaggtcaa
    22261 cgacaagatc gccgaagacg tccgcgaggg catggagaag acgcagaagg agttcctgct
    22321 gcgtcagcaa ctcgccgcga ttcgcaagga actgggcgag ggcgaacccg atggctcaga
    22381 tgactaccgg gcccgcatcg aggccgccga gctgcccgaa aaggtccgcg aggccgcgct
    22441 gcgcgaggtc ggcaagctgg agcgatccag tgagcagagc ccggagggcg gctggatccg
    22501 cacgtggctg gacaccgtgc tggacctgcc gtggaacgtt aagaccgagg attcgacgga
    22561 tctgaaggcg gcgcgcgaga ttctcgacgc cgatcaccac gggctggaag acgtcaagga
    22621 ccgcatcgtc gagtacctgg ccgtgcgggc gcgtcgtgcc cagcgcggca tggcggtcgt
    22681 cggcggccgc ggctccggcg ccgtgatggt gctggccggt ccccccggtg tcggcaagac
    22741 gtcgctgggc gaaagcgtcg caagggcatt gggccgcaag ttcgttcgcg tcgccctcgg
    22801 tggcgtgcgc gacgaggccg aaatccgtgg gcaccggcgc acctacgtcg gcgcactgcc
    22861 cggccgcatc gtgcgagcga tcggcgaggc gggctcgatg aaccccgtcg tgctgctcga
    22921 cgagatcgac aaggtcgggt ccgactaccg cggcgacccg agcgccgccc tgctggaggt
    22981 gctcgaccca gcgcagaacc acacgttccg cgaccactac ctggagctcg acctggacct
    23041 gtccgacgtc gtgttcctgg cgacggccaa cgtgatcgag aacatcccgt cggcgctgct
    23101 ggaccggatg gagctgatcc agatcgacgg ctataccgag gacgacaagg tcgcgatcgc
    23161 gcgtgacttc ctgctgccgc gacagcggga ccgtgcggcc ttgaccgagg acgaggtcac
    23221 ggtgaccgag gacgcgctgc gcaagatcgc ggccgactac acccgcgaac cgggagtgcg
    23281 gcagttcgag cggctgttgg ccaaggcgct gcgcaaggcc accacgaagc tggccgagaa
    23341 tcctgaagtg ctcacgatcg acgagccgga tctcgttgac taccttggcc gtccgagatt
    23401 catgccggag tcggcggagc gcacggcagt ccccggtgtc gcgacgggct tggcggtcac
    23461 aggtctcggc ggcgatgtcc tctacatcga ggccggcgcc accgacggtg agccgggtct
    23521 gcagctgacc ggtcagctgg gcgacgtgat gaaggagtcc gcgcagatcg cgctgtccta
    23581 cgtccggtcg cacgccgagc agttaggtgt cgatcccaag gcgctggacc gccggatcca
    23641 cgtccacgtg cccgcaggtg cggtgcccaa ggacggcccg tcggccggtg tgacgatggt
    23701 gaccgcgctg gtgtcgatgg ccaccggacg ccaggtgcgc tccgatgttg gcatgacggg
    23761 cgaagtgacg ctgaacggcc gcgtgctacc gatcggcggg gtcaagcaga agctgctggc
    23821 cgcccaacgg gccggtctgt caacggtttt catcccacag cgcaacgagc ccgacctcga
    23881 tgacgtgcct gccgaggtgc tggaggcgct ggaagtcaag ccgatgaccg acgtggctga
    23941 catcgttgcg ctggcgctgg agcctgccgc cgacgccgcc actgtcgcgg cctgacggtt
    24001 caccgctgac gcgcaagagt ccccatttct gcgtgaaatt ggggactctt gcgtttgttc
    24061 ggcacattcc tcatcccggc taaggcgcgt caaacgcacc atgcgattcg tagtgctgga
    24121 ctccttccgg cttggtcagc aggtagcggt cgtcctccgg gtagtacacc gcccgctcga
    24181 cgtcgtcgcc ggcgaatccg cgaatcgcat ccatcgatgg ccaccaactc aatgtgatga
    24241 cctccgcgcg ttcgtcgtcc agatcgcgaa ccgcaatgag cgcgccgaga ttgcctggcg
    24301 tcgcacggta atcggcgagg ccggtgtcgc gcatgtaggc gacgtattcg ccgaccagtt
    24361 cccgacgaag ctcgccccgc cattctcgaa ggattgtcat agccaccccg attcagtggg
    24421 atttaattcg gccacggaag agttccacac tatcgacgta gccgttcgcc gcacagccgt
    24481 atcagccacc gaccggccat attgcgacgg ccgctgtacc cgagggaggg gcattgccca
    24541 aacgtggaaa gccgctgcgc aaactagggt tcctgaccat cggccgcttc gatgcggccg
    24601 acccggggcc cgggcacgag cagacgctgt cgatgatcga gcgtgccgaa gcgctcggct
    24661 tcgacagcgt ctggctgcgg tgtcggcacc tgcagccggg catctcgtcg cctgtcgcga
    24721 tgatggccgc cgccagtcag cgcaccaccc acatcgaatt gggcacggcg gtgatcccgc
    24781 tcggcctcga gaatccgctg cggctggccg aggatctcgc gaccgtcgac atcctgtcgg
    24841 gcggccggat caatccgggg gtgtcggttg gcacgccgat gctgtacgac aactacaaga
    24901 ccacgctcta cccggaaacc catgaactgg agaacttttc aaaggatcgg gtggtgcgcc
    24961 tgctcgagtg cctgcgcggc gaaccggtca gcgatttcga gggcaaggtc ggcatcgagg
    25021 acttcacccg ccgagtgcaa ccccattcgg cgggactcgc ggatcgggtc tggtacggcg
    25081 gcggcatgtc gtccgcgatc tgggcgggcc aacagggcat caactacatg accagttcgg
    25141 tggtcagcat cgaaggcacc gcgtcccgcg acttcgccac catccagggc gagaacatcg
    25201 acgcgtaccg cgcccaccat cccgagccgg aaagggcgcg cgtctctcag ggcctcgtcg
    25261 tcattcccac cgactcggct tctcccgaac aggtcgagaa gtaccgggcg tatgcggcct
    25321 ctcggttcga caggaccaag gcaccgcaag gcgcgcgcgg catgctcttc tcccccgatt
    25381 acgtcggcac gtccgacgaa ctggccgaca cgttgtacgc gcatgcgggc ttccagcgcg
    25441 ccgacgagat cgcattcgcg ttgccgttca ccttcgacga agaggactac gtccagatcg
    25501 tcaccgacat ggccgaaaag ctcgggccga agctggggtg gacgccggcg caggcgtgaa
    25561 tgtcggtcgc gcgcgctacc gtcctcgcat ggcctatgac gaagacttgg ccgaccggat
    25621 ccgtgaactc atcgccgccg aacgtggcgt cgaggagaag cggatgttcg gcgggctcgc
    25681 gttcctgatc aacggcaaca tgtcggtcgc cgcgagcggc aagggcggtc tgatggttcg
    25741 ggtgccgccg gacgagaccg agaagctgct ggcccgcgat cacgtcgaac ccatggtgat
    25801 ggctggccgc gaaacacgag gctggctgcg ggtatccggc gacggcgtca agagcaaacg
    25861 ccaattgcaa tcctgggtcg gccgcggtgt cgactacgcc aggagcctgc ccccgaagtg
    25921 acggctcagt cccgcgacgg gtgtttgatc aacacgtcgt actgcgagtt cgccgtgtcg
    25981 atatctcggg gctgaaactt gccctccggg gtcaggatcg gcatgcccat cgcgtcgcgg
    26041 agttccccgt tgcctgcgct gtggacgaat accgcgttcg gttcgggaag ccggggcggt
    26101 aggcccgggt ggtcgacgaa ctgtagaccg ctggccgcga agtaggtcac cgacaggctg
    26161 gccgcgtccg ctttctgaaa ccacgcttcg gccaacacgt tccatcgctg cgattcggcg
    26221 ctggcctcag atgctggaat gcccgacgcg accatctgtt ggtatgaggg ccagccgatt
    26281 tcgccgatat cgccgcggac ctggtttctg gccaaccaat cgatgaactt gtcgaggcgg
    26341 tgcagattcc actcatcgaa cgagcgttcg cccgtagtgg acttcgcctc aaatcgattg
    26401 gcccagaagg aatatcgcag ttcgaggggg ccgttgcctg cgacggtgta tctacccgtc
    26461 tggtcgaagt actggtgcgc cgagtacacc accttgccgc gcggatcgtt gatccacgcc
    26521 ttgggatgaa cgtctccgaa cgtatccaca ggccaatgcg cgcttcaggt tgagtcttgt
    26581 cagcagaacc ggcaacgcaa tgctcggttt gaccagggcc agcggaagaa gctgcggcag
    26641 atagtaggtc gcgagtatca ggggcgccca ctgcacatag accacacagg cgacgtaggc
    26701 cggtgaggcg aaggtcagca gcggccacag cttgccttct ttggtcagcc cataagccag
    26761 caatgccgac atcgcgccga agaagaccgc ggcgataacg gcgtcgggtg cccaggtgaa
    26821 cggcaggaac ggcatcgcgg tggtgaccgg gttcgtcggc cacaggttgc cggcgtcgtc
    26881 gactgtgacg ggctgaacgt atgggtcgtg accggcgatg atgtcgcggg cggcctgaag
    26941 gggccaggcc atgtcccctg cgcccgtgcc acgcgccacc gacgcgacgt acatcgcaac
    27001 accggcgagc acaccgatcg agacgaccag actccaccgg accctaacgg acatagtgtg
    27061 ccattcctcg atcagcgaac tgcttttagt gcatcgaacc atattgttga caaatcagct
    27121 actaaagaga tcttcgctca tgagggcggt tgacggtgct gtcgcgtttc ttgggtggtg
    27181 cagcgggtac gtcacgtcaa gtgggcaaag caggggcgca agtgcggatc ggtacctccg
    27241 ggtggtcgta cgatcactgg gccggcgtgc tgtatcggcc agggctgccg gtcgccaaga
    27301 ggctggcccg ctacgtcgag gagttcgaca ccgtcgaact caacgcgagc ttttaccgct
    27361 ggcccaagga cgccacgttc tccggatggc gagagcggct tcccgaaggc ttcaccatgt
    27421 cggtcaaggc gcaccgggga ttgacgcact accggcgatt aggttccccg gagccgtggg
    27481 tggagcggtt cgagcggtgc tggacggccc tgggcgaccg gcgcgaggcg ctgctcgtgc
    27541 agctgcatcc cgggatggaa cgcgacgatg cgcggcttga tcacttcctg actgtgatgc
    27601 ccgactggat acccgtcgcg atggagctgc gccatccgtc atgggatgac cccgccgtct
    27661 acgcgacgct ggaacgacat cacgccgcat acgtggtgat gagcggcgcc aaggtggcga
    27721 cgatttcccg ggccaccgcc gacttggtat acgtgcggat gcacgggcct gcccaggact
    27781 cgatgtatca cgggtcttac cccgacgatg agttgcgcca gtgggccgat cgcgtcgccg
    27841 aatggcacga cgaaggccgc cgagtgctcg tgtatttcaa caacgacctg ggcggccacg
    27901 cgattcgcaa cgcccgaaag ctcagggagc tgctggcggc accgagtagg ctcaggtgat
    27961 atgacaacgt cgtccacctt cgccatcgtc ggcggcggcc tcgccggcgc caaggccgca
    28021 gaagcattgc gcgacaagga tttcgacggc cacattgtgt tgtttgccgc cgaggagcac
    28081 ctgccctacg agcgcccgcc gctgtccaag gagtacctgg ccggcaagaa ggagttcggt
    28141 gacttcaccg ctgcgtcatc ggcctggtat cgcgaccacc acgtcgagct gcagctcggc
    28201 acggaggtgt ccgccatcga cccgggtgcc cacaccgtct cgctgcccga cggcagcacc
    28261 gtcggatacg acaaactctt gctcgccacc ggctcgcacc cgcgccgacc accgatcccc
    28321 ggcgcagacg ccgacggcgt gtactaccta cgcaccgtcg acgatgccga cgcactgaat
    28381 tcggtgctgt ccgaaggttc ttcgctggcc gtcatcggcg gcggttggat cggcctggag
    28441 gtggccgccg gcgcccgaga ccgcggcgtc aacgtcacgg tcgtcgaaat ggccgagctt
    28501 ccgctgatgg ccgcgctcgg tcgcgaggtc ggcgaggtgt tcgccaccct gcaccgcgaa
    28561 cacggtgtcg accttcggct gggagcaacc gtgcaggaga tcactaccgc aggcggcaaa
    28621 gcgaccgggc tgaagctcgg cgacgggtct accgtcgacg ccgacgccgt ccttgtcgcg
    28681 gtaggcgcgg cccccaacac ggctctcgcg gaacaagccg gcctcgcgct cggcgacggc
    28741 ggcgtgcttg tcgacgcgtc cctgcgcacc agcgaccccg acatcttcgc cgtcggtgac
    28801 atcgccgccg ctgagcaccc gctgttcggc acacgcatcc gcaccgagca ctgggccaac
    28861 gcgctcaagc agcccgccgt cgccgcggcc ggaatgctgg gcaagccggc cgaatacgac
    28921 gaactcccct acttcttcac cgatcagtac gaccttggga tggagtacgt cggccacgct
    28981 cccacctacg cgaaagtggt gttccgcggt gacgtcgcca agcgggagtt caccgcgttc
    29041 tggctcaacg ccgacgaccg ggtgctggcc ggcatgaatg tgaacatctg ggaaggactc
    29101 gacgacatca agtcactgat ccggtcccgt gagccgttgg gcgccgcttg atccgccgag
    29161 atgtagccgc atttttcgcg gtggccatcc ttgctgccgg gtgtgcggat gaacccatca
    29221 tcaacacacc ggagccgcca gcccagaccg gctccgccgc gccagcggcg ccgtcgacac
    29281 cggccagcaa cgcgcatctc gctaacgcgt tcgactacgt cgcgcacgtc ggcgacacca
    29341 cgggctacta cttcaccacg cccagcggca agtggcggtg cgcgattctg acgcacctca
    29401 aggcgggctg tcaggcggcg agcagctggc aatcagggct cggcgtcccc gatgagccgg
    29461 agtcgacaaa ctcgatcgtc gtcgaccgcg atggtgaccc acagttcctc gcgctggaac
    29521 agcccgagtt ctggcccaat ccgggaccgg cgaagacgtt gccgttcaac aggatcctgg
    29581 cggcagcggg ctttcgctgc aacgtgcagg agtccggcgt gtcctgtcag agcgcggcga
    29641 gcggcaaggg tttcacgttc tccgcggagg ggttcacgcc gcaatacacg gacgtgcctg
    29701 ccgacgcgcc gtaaccgtca cgatgccgtc gcgacgggtt cctttctgtc ctcggcgtgg
    29761 gctacctcgt cgagcggttc aacggtctgc ccgcgcaaca ccttccgcat ccggtccgtg
    29821 tcgagctggc cttcccaacg cgcgaccacc agtgtcgcca cacaattgcc aagcaggttc
    29881 accgagaccc gcatcgagtc catgatccga tccgccccga gcaacaacgc caccgccgcc
    29941 accgggatcg caccgtgtcc gatcgctgcc accgtcgctg acagcgccaa aaacgacgag
    30001 ccgggtacgc ccgacatgcc tttcgacgtc agcatcagca caagaatcgc ggtgacctgc
    30061 tcgccgaggc tgaggtccac cccaagcgcc tgcgcgagga acagtacgca gatcgacagg
    30121 tacagcgtgg ccccgtcgag gttgaatgaa tagccggtcg gcaccaccaa acccgttgtg
    30181 gtccgggaac atccggtgtc gaccagcttg accatgatgc gcggcagcac gacctcggtc
    30241 gacgccgtgc ccagcgcgag gaacagctcg tccttgatgt acaccacgaa ctgccacagg
    30301 ttgacccccg ccagaaacca ggcgattgcc gcaaggacga ggatgaagaa cgccgcaccc
    30361 aggtaacagg cggcgatgag tttcgcgtag ctgcccagtg atgagatgcc gtactgaccg
    30421 atgatgtagg ccatcgcccc gaacgccgcc accggcgcca gtcgcatgat ccagccgatg
    30481 accgtaaaga agatgctgct gaggtgctcg atcagctcga agaccacagg tggcccgtgc
    30541 tcaccgaact tcgccagcgc cagcccgaac aacaccgcaa agaacagcac ctggagcaac
    30601 atgttctcgg caaacgcgga aaacacggac gtcggaatga tgttgaggat gaactcgacg
    30661 gtgtgcggca gttcgccgtc cccggtcttc ttggccactg cctccgcgcc ggtcgccagc
    30721 gtctgcgggt cgatgttgaa acccgcgcct ggcttgacga ggtttccgat gaccaacccg
    30781 aacagcaacg cgaacgtggt gacgacctcg aagtagatca gcgccttgac cccgatgcgg
    30841 cccaccgact tgaggtcgcc gacgtgggcg atgccgatca cgaccgtgca gaagatgatc
    30901 ggggcgatca gcatcttgat cagcttgatg aagccgtcgg ccaggggctt gaggtcggcg
    30961 ccgaatgcgg gccagagcca gccgacagcg atgcccgcca cgatcgcgac cagaagctgg
    31021 acgaacagcg gcgagtccca ggccttacgc ggagccacgg agacatctgt cattggcatc
    31081 acgtaagtga cagcatgtgt cggaaccata agcgccgtgt taccggcgcg ctaccggcga
    31141 aatatattgg accggcatga ggatcgagac gggacaggtc gccgtggtga ccggcggtac
    31201 cagcggcatc ggcttcgcga tcgccgaggc gctggtgcgg cgtggtgtca acgtgatgat
    31261 cgccgatatc cgtgaggacg cgattccgct cgccgtcgac gcgctggccg gtcagcgcgg
    31321 gcacgcggcc ggggtgcggg ccgacgtcgg tgtcgacgcc gatgtcgacg cgctcgccga
    31381 cgagaccctc gaacggttcg gccgggtcga tctcgtctgc aacaacgccg gcgtggtctg
    31441 tgaacaggca ccgacttggg aacagcgacc ggcaacgtgg cggtggctga tcgacgtgaa
    31501 gctgatgggt gtcgtcaacg gcgtgcgtac cttcgccccg ttgctcatcg cgcagggctc
    31561 cggccatttc ctgaacaccg catcggcagg cgggttgatg gcgctgccga cgctggcccc
    31621 ctacgccgcg accatgcatg cggtcgtcgg gctgactgag acgctgaatg ccgaactacg
    31681 ttcggtgtca acgcttttag gtgcgacagt gctgtgcccc gggctggtgg acacggcgct
    31741 gggagccaat tcggtaagac tggttccgcc gggtgcggcc accacaccgg caaatgcggg
    31801 tggcatgcct gccggcgcga tcagcccttc ggtggtcgcg caagcggcaa tcgaagccgt
    31861 cgaggccgat cgggtgcatg ccatcgtcgg ccccgccaca gatgtggtgg tccggcagcg
    31921 ggtagacggg ctgctggccg acctcggcta gagcagcgtc gcggtgccgt cgctgttgac
    31981 ctgcaccttg acgccggcga tgtcttcgcg aatggtgacg tcggcctccg ccggatcagg
    32041 gatcaccgcg agcacccgcg cgtcgtcgcc agtgcgcacg gccaggacgg ccttttctgc
    32101 ggtaccggtc cggctgaacg gcgtggtcca tgcctccacc gtgccaacac cggaccactc
    32161 gacgacggcc ttgcgagtcg gttcccggtc gacgtcggat tgcacatctt cccaacggaa
    32221 ctcgtggctc gggggctccg caccgtacac gccgaagctg tgcttggtca ggtagccgcc
    32281 attggcggtg atgaagcccc gctggcccgg gtttgcgacc aggcgctcgg ccaacgtggc
    32341 gatcgaatgg gtcacgtagt tgttccacgg cccgccggcg aacgtcagac cgccggtgac
    32401 ggtcagcggc cggttcgggt cgccgaccgg cagccccagt tcgttggcgg cgacttgcac
    32461 cgcggacgga aagcatgagt agacgtcgat ctggtcgagg tcgtcgactc cgacgccggc
    32521 caactgcatc gcccgttgcc ccgcaaccct gatcgcgggc gagccgtaga actccgcccg
    32581 ctctccgatg gcgtacgtgt catgcgcgtc ggtgcctgcg tacgggaaaa cccaacggtc
    32641 tgaggggatt tgcagatacg tcgccttctc caccgaggtg agcaccaacg ccgcaccctg
    32701 atcgaccatg ttgttcgagt tcatcagctt ggtgtacggc cagctgatca tccggttatg
    32761 cggactgggc tgccagatct gatgcggcgc caacggctca cggctccacg cgtgcggatt
    32821 cttggccgcc accccgctga actgtgacca cagttcgccg atccggcgca ggtgctcttc
    32881 tgctgtttcg ccattggcga tccgcagggc ctgctcgaac atgggataga caaaggccgg
    32941 ccgatccaat ttgatccgca gctccgcggg cccggccatc ggcacgcctt catcgacgcc
    33001 ttcggccatc ggaaccgtct cgtcctggct cgtccagtcg ggcttggcgc cgctcgcacg
    33061 caatcgcgtt cgggtgcgcc aggtttcggc gccggcgatc agcactgcgt cggcacggcc
    33121 ggtctggatg tccaggcatg cctggttgac gagagtctgc ggcacgttac cgcccactcc
    33181 ggtgtagcgg gtcgcggcgt ccttggcacg caggcgttgg gccagcagca gccctggatc
    33241 gcgatagcgc cacgacagca cgctcaccac acggatcgag tcgacggcct cgagcacccg
    33301 aggatccgct gcggcgcggg cggccgccac catcaggtcg actggctcca cccctggatg
    33361 gtcgtcatgc tggttgacct ggccgtagcc gatgagtacc ggcgtcctgg ggtcgatggg
    33421 catgggtact agtttgacgg gccggcccga cagatcgata tcagggtctc ccccgatgag
    33481 tgaacaggac gccgtcggtg atcagtcgat cgatctcatc ggcgttcatg gcgagcagtt
    33541 tctggcagat ctcgcgggtg tgctcgccgg gcatcggtgc gggccgaagc tcggcgggtg
    33601 gaatgttccg gtacggcgcg ggccgggttt cggtgggcat cggcgcgccg aacagcggat
    33661 gagtcatgtc gctgaacagt tttcggaatg tgatctgcgg atcggccagc acatcgacag
    33721 cgcggttcat cggcgcggcg ggcactcccg cgcgctgtaa taggtgggct acttccgctt
    33781 tgtcacgcgt gcgcgtccac tcggacacgc cagtgccacc ggttactgag gacagcgctg
    33841 cgcggtcgtc gtcgtcgcgc agcgatatca cgcaccactc gtcgtcgccg tcacacggat
    33901 acacgccgtg cactgcagtg tcgtcggcca ccggcaggtt cgcagcgcgc gctgcctcgg
    33961 cgacataggc gggagcaagc tggtttaccg ccgcttcggc ttgcgaaatg tgcacgtgcg
    34021 caccggttcc ggtgcgggca cggccaacca cggcagccaa cgcggcgatc gcggtgagcc
    34081 tggccgagac gtggtcggga aagatcgtgg tggcgtcgta gaaactgttg cgagacgcat
    34141 gatcggcgct ccacagccag gtcaccccgg tggtggcccg caccagcggg ccgtatccca
    34201 gccgcgcact ccatgggccg gtggccccga acgcgctgct ctcggcgagc acgatacgcg
    34261 ggttgagctc gcgcaggcgt tcgtaggaaa accccagcgc cgcaagcgtc cccggcttga
    34321 agttggcgaa taccgcgtcg gcacttgtga ccagtttctc gaacagagcg gcgccggccg
    34381 ggtgtcgcag gtccagaccc aggctgtatt cattgcggtg ggtcagcgcc caggatctgc
    34441 tcatcacctg gcccggcggc gtctggcgca ggccgtcggg ataggcggcg ctctcgactt
    34501 tgatgacctc ggcgccgagg tcggcgaaca gccggcccag ttccccgcct gccacgatca
    34561 ccccgaggtc gaggattcgc atgccgtcaa acggccggcc ggccgcaccg ctcaccactg
    34621 cggtttcgga acgctctgtc gcccaggtgg gttcgtctgc accgacggac ggcgcggtgg
    34681 tcgtgtagcc gacatgctca ccatcgacga cgaacggtcc aaccggaacg gagacaccgg
    34741 cttcgacgtc ggtcagcgcc ccgaccgcac ggaagtgctc cgacgacagc gcctgcgcgg
    34801 gcgtgagcac cgcggcaatc ggcacgccgc gacgttggcc ctcggccacc aggtcgtgca
    34861 tcgcccccgc ggcgaacagc tccgcgatgg cctcgttcag ttcacgcgac gccgcgtagc
    34921 gcgccgcgat ggtgtcgaac ttcggatcgg cgaaccgctc gggttcaccg agccacgcgc
    34981 gcataccccg ccactgccga ggcgagagca ggcagatccg cacgtatccg tcccggcacg
    35041 gaaatatcgg gtagatctgc tgatttcgcg gcctgccccg ccacagctcg taggaccgct
    35101 tcacgccgac agccgcctgg ccctcggatc cgaacggcgg atcgagtgac tgcacaaccg
    35161 cctcgaatcg cgaaaagtcg atgtattcgc ctgtgccgca acgtaaccgg tggtagtagg
    35221 cggccagggc ggcccacgcg gcctgcacgg cggcggtcgc cgatgcgact cccagtggcg
    35281 gcagcaccgg tgttcccgtc gtcggcccgg tccgcgacaa cgcggtcgac atggcgtaca
    35341 gcaccggatc ggtcgcttgc catgaggcgt atggacctga cgtcccgaag tcggtgacgg
    35401 acaaggcaac cagatggccg aaccggtcgg ccagcgccgc acacgacgtg ccgtacgcgg
    35461 ctgcgcgacc gccgccgtcc accacgatgt cggcggtgcc ggccagctcg accagacggt
    35521 cacggtcggc tgcactgtcc ggatccagca cggcgctgcg cttgttggcg ttgtgcagtg
    35581 cgaacgggat gctgacgccc gccacagcgg gcggcgcgtg gcgcgccggg ctgcccgcag
    35641 gcgactcgat cttcagcacg tcggcgccga ggtcggcgaa caacctgccc acggcgtcgg
    35701 attcggcacc gccgagatcg agcacccgaa gctgcccgag caggccgttg ctcaccgggt
    35761 cagcaaccgt tccacccagt ccaggtgggc gtctgtcccg gtcgccacat acatcaggtc
    35821 gatgaacgcg tccgggtagc cgttgtcggc cagcagcttc acggcgtcgg cgaccttgtc
    35881 caccgcagtg ccttccgcga cgttgacccg cacataggtg tggatcgccg acggatcgcg
    35941 acccgccgcc cgcgccgcgt cgtcgatcgt ctgccgctgc cagttgagca tctccaggct
    36001 cactacgtcc ggcacgctga cgccggccat ccagccatca gcgcgctcac cgacccgctt
    36061 gagtccgggc gccgtgaatg cgccgaggta gaccggcgga cgcggccgct ggaccggctt
    36121 gaggtcgacc cacgattcgc cgatcgtcca gcgctcaccg tcgtgccgag ccgggttggc
    36181 ggtccacagg gcgtcgagcg cgtcgaggat ttcatcgagt tgggcgccgc ggcggtgaaa
    36241 cggcgcgcct gcggcttgat actcctccgg agaccagccg atgccgaacc cgggcagcag
    36301 ccgaccgccg ctgatcacgt cgatgctcgt caattggcgg gccagctggg cgggcggata
    36361 ccagggcgcg acgaacacgc tgctgcccag ccgcgccgtg gtggtcgccg ttgcggccac
    36421 cgtcaacgcg atgaatggat ccaggttggc gcggaactgc tcggggatgg tgtctttgcc
    36481 gccatagccc accgtggggg tgacggccgc cagcagccgg tcccccaccc agaggctgtc
    36541 ggcgccgagt tgttcggccg tcgacgcgaa ccgggcaagc tctccggggt ccaggtgggc
    36601 ggactgtccg aactgcggca ccgcgaatcc gagcacgacc gacaggctag tgctcgaagt
    36661 ttgacgattc gggtacttgt cgccctagcc gttgttcacc tgccgagacg atgatgagcc
    36721 agatgaccac ccaggccctg tcccccacgc tttccgactc tgaactcgac ctgatcaacg
    36781 cctactggcg ggccgccaac tacctgtcgg tgggccagat ctacctgctg gacaacccgt
    36841 tgctgcgcga accgctcgcg cccgagcaca tcaagccgcg cttgctcggg cattggggta
    36901 ccacacccgg gctgaacctc atttacgccc acctcaaccg cgtcatccgc caccgcgacc
    36961 tcaacgtcat ctacatcacc ggccccggtc acggcggtcc cggccttgtc gccaacgcct
    37021 acctggaggg cacatacagc gaggtgtatt cgggcatcga ggaagacgtc gacgggatgc
    37081 gaaagctgtt ccgccagttc tcatttcccg gcggggtgcc cagccacgtc gccgcggaga
    37141 caccgggatc gatccatgag ggcggcgagc tgggctatgc gctggtgcat gcctacggcg
    37201 cggcgttcga caacccggat ctggtggtcg cgtgcgtgat cggtgacggc gaggcggaga
    37261 ccggcccgct tgccgccagc tggcattcga acaagttcct caacccggtg accgacggtg
    37321 cggtgctgcc gatcctgcac ctcaacggct acaagatcgc caatccgacg gtgctggccc
    37381 gcatcccgca ggaggagttg gagtcgctgc tgtacgggta cggctatcgg ccgatcaccg
    37441 tcgccggcga cgatccggcc aacgtgcacc agcagctggc gacggcgctc gacgaggcat
    37501 tcgatcagat cggcgcaatc cagcaggcgg cgcggttgga cggcgagact ggccgcccgc
    37561 tgtggccgat gatcatcatg cgcaccccga agggctggac cgggccgaag gaggtcgacg
    37621 gcaagaaggt ggaggacacc tggcggtcgc atcaagtgcc gctgtccgag acgcactcca
    37681 accccgaaca catggcgcaa ctcgagacgt ggctgcgcag ctaccgcccg gaggagctgt
    37741 tcgacgaaca cggtgtattt cggccgcagc tgcgggctct cgcgccgacc ggccagcgca
    37801 ggatgagcgc caacccgcac gccaacggcg ggctgctctt gcgcgacctc gacctacccg
    37861 acttcaccga atacgccgtc gcggtagaca atccggcggc cggaaccgcc gaagccaccc
    37921 gcgtgctggg gacattcctt cgcgacgtga ttgcccgcaa cccggaccgg ttccggctga
    37981 tggggcccga cgaaaccgcg tcgaaccggc tcgcggcggt gttcgagacg accgacaagg
    38041 cgtggttggc cgagaccgaa cccgacgacg agaacctcgg gccggagggc cgcgtgatgg
    38101 aggtgctctc cgagcatttg tgtcaggggt ggctcgaggg ctatctgctc acgggcaggc
    38161 acgggttgtt caactgctac gaggcgttcg tgcacatagt cgactcgatg ctcaaccagc
    38221 atgccaagtg gctgacccgc aaccgggagc tgccgtggcg gcagccgatc gcttcactga
    38281 attaccttct gacgtcccat gtttggcgcc aggaccacaa cggcgcatcg catcaggacc
    38341 ccggtttcat cgaccacgtc gccaacaagc ggcccgaggt ggtgcgggtg tacctgccgc
    38401 cggatgccaa cacgctgctg tcggtggccg atcactgcct gcgcagccgt gactacgtca
    38461 acgtgatcgt ggcgggcaag cagcccgcgc tggcgtatct ggacatggag tccgcgatcg
    38521 cgcattgcac ccgcggcctc ggtatctggg agtgggcagg cacggccacc ggggagcccg
    38581 atgtggtgct ggcctgcgcg ggtgacatcc cgacgttgga gacgctggcc gccgtcgata
    38641 tccttcgccg cgagctgccg gagctggcgg tgcgcgtgat caacgtcgtc gacatcatgc
    38701 ggctgcaacc ggactccgag catccgcacg ggctgtcgga tcacgaattc gactcgttgt
    38761 tcaccaccga caagccggtc atcttcgcct atcacggcta tccctggctg attcaccggt
    38821 tgacctatcg tcgggccaat cacgcccaga tgcatgtgcg tgggttcaag gagcgcggca
    38881 ccacgaccac cccgttcgac atggtgatgc tgaacgacct ggaccggttc cacctggtgc
    38941 tcgacgtgat cgatcgggtg cccgggctgg ccaaccgggc cgcggtgttg cgccagcgga
    39001 tgctcgatgc gcggttggcc gcccgccgct acacccgcga gcacggcgag gacgaccccg
    39061 gcatctcggg ctggacctgg gactgggacc ggtagactgg cccgcaatgt ctgagcaagc
    39121 cctgtccccg cacccccatc ccgtggtggg ccagcttgcc gcattgcacc acttccgtac
    39181 ttacgtcgat atcggcgtgg tggtggtggt gctggcactc accaacctga tcgcgcattt
    39241 cacgacgccg tgggccagca tcgcgaccgt gcccgccgcc gcggtcgggc tgttgatcct
    39301 ggtgcgttcg cggggcctcg gttggtccga actagggctg agtcgtgagc actgtaaggc
    39361 cggagcgggc tacgccctcg ccgctgtcgc ggtggtcggc tcggtgatcg cgatcggcct
    39421 gctgttgccg tggacccggc cgatgttcat gaacaacaac tatgcgacgg tgtcgggcgc
    39481 gctgatcgca tcgatgatca tcatcccgct gcagaccgtg atccccgagg agctggcgtt
    39541 ccgcggggtg ctgcacggtg cgctgaatcg cgcgtggggt ttccgcggcg tggcggcggc
    39601 gggttcgctg ctgttcggct tgtggcacat cgccacgtcg ctggggctga ccagcagcaa
    39661 cgtcggcttc acccggctgt tcggcggcgg cttgctcgga accgtcgccg gggtggtgct
    39721 agccgtggcg gcgacggctg ccgcgggctt cgtgttcacc tggctgcgca ggcgcagcgg
    39781 cagcctgatt gcgccgatcg cgctgcactg gtcgctgaac gggctgggcg cgctggccgc
    39841 cgcgctggtg tggcacctgt cgacctagcg cgtcttgctc ctggcgcgga acgcgcggtt
    39901 cttcagcttg ttgccgcaca ccgacatctc acaccaggtg ccgccgtgat tgcgggatct
    39961 gtcgtagaag gcccactggc attcgtcgtt ggcgcaggcc ttgagcagct tccaggtgcc
    40021 atcgcgctgg gcgtcacgga cgatcagtag caggtccacc aaccggtcgc gcacggagtc
    40081 gccggccgcc agcaatcgca cttcgccgtc gtcggccaat gatgcgcggg ctgtgccgcc
    40141 gtccgcgacc gaccgcagca cggccagtgc cgatttaatc ggtggcggac cgcccgcgtt
    40201 gtgtaccagc atcgctcgta gcgcttcgcg tacagcgcgg acaacttcga ggtcagcgtc
    40261 ggtcagcgca gccttgggcg caagcaggcc ggtgtcggcc aaccatggac gcgcatcggc
    40321 cggatcagcg agccggtcgg ctccgtcagg cagttcgacg gtgttcacca gcgactgaac
    40381 gagcgctagc gggccgggcg ccggcttccg ttcgtcgtcg ccgggccagt ggacaggcgc
    40441 tggggtcatg tgagcagtct acccgctgca tgaccggtat aacagtttga ctggttatgc
    40501 atgaccggtg tagcgtgttt gatagtcacg aagttcaaga gcagatacag gcaagaggta
    40561 gatgaccgtg ccgaacaaca agatttcgcc ggatcttctt tcgaccgcga gcgacgcgct
    40621 gcggcggacc gctaccccgc ggccgtcact ggcggcgcgc gtgacggcgc gactgcgggc
    40681 ccaccaactc gaccggcagc tcgctgtcgg cgtgcctgcg ccggcaggaa gcgcgctcgc
    40741 cgttcatcag gcgcggttgg tcacagtggc cgagcgtgaa gccattgcac gcacgctgcg
    40801 gcaggcgctg gacgacgcga atgcgggcgc tgcgccgctg tcgtcacgga ttccattgca
    40861 ccccaccaac attgtggcag cggaggatct catcgacgcc atcacgctgc ggctgcattc
    40921 gccgcggccg gtgagcccac ttgggatggc gcggctgcgc gtcgtgctgt ccgacgggtc
    40981 tggaccgatg taccggttcg gtcgcggtga tctcagcggc cgcctcggcg ccgcactcgc
    41041 ggagctctaa acggacccgg ccaggacgtt ggtcaaatcc ctgtgcacaa cgatacgaag
    41101 gggatgggcg cgcagatgcc caccgagaag tacggcgtca ggtcaccgtt ccatgccggc
    41161 ggcggtggag ggggtggtgg cggaagtggt gcggcgacgc aagtgttcga gggcacgtca
    41221 tacacggtcc ccacgccgca ctcgactgcg ttagctctgc ccggtgagac cagcgtcagg
    41281 gatgcaagcg gcgtgagaat tgccgcgacg acgattgtca gatggacaac tttaccccgc
    41341 attttgaggc ctccctactt ccccgacctc ggcgtatctc acatcataag actcgccgga
    41401 aaccggttca atggaattga ccaccgcgta aaaccgggag cagctcgggg ttcagaagct
    41461 atgccagcca ccagcgatgt actgcttccg cgaccgcgtc gttgcgcaag ctgaccgcgc
    41521 tggagtgccc ccgacaagca actcccgacc gcgggtcttt catttcaggc ggccaagcct
    41581 gctgccaacg acagaagggt atgtctagac catcaaaacc gccgcgccgg cgatgcggcc
    41641 ctcactcaga tcggacagtg cgtcgtcggc gcgaccgagc ggatactcgg gcgtcgtcac
    41701 ctcgatgtgg tgctggcccg caaacgcgag aaactcccgc gcatcgttac gggtgttaga
    41761 ggtcaccgat cgcacttggc gctcctcgaa gaggtggcgt tcatagttca gagcagggat
    41821 gtcgctgaga tagatgcccg cgatggccag ggtgccgccg cggtcgagcg cctcgagggc
    41881 gggcagcacc agatcaccga cgggcgcgaa caggatcgcg gcgtccagtg ccaccggcgg
    41941 gcgatcggtc gcgccctgcg ccgaggccgc ccccagtgtc agcgccaact cgcgggcttg
    42001 ctcaccgcgg gtcatgacgt gcacctccgc gccctgcgcg agagccacct gggcggtgag
    42061 atgtgcactg ccgccgaacc cgtagatgcc caggcggccg cccgcgggca ggtcggcccg
    42121 caacagtgag cggtagccga tgatgccggc gcacagcaa
//
LOCUS       DASRJA010000645         4278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1819997,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000645 DASRJA010000000
VERSION     DASRJA010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819997"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..620)
                     /locus_tag="VFC01_29040"
     CDS             complement(24..620)
                     /locus_tag="VFC01_29040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN83699.1"
                     /translation="MKADASVVDKTPGRPRDPRIDAAILRATADLLVEIGYSNLTMAA
                     VAERAGTTKTALYRRWSSKAELVHEAAFPAAPTAIETPPGDIAADIHAMIAAARDVFT
                     SPLVRAAMPGLIADMAVDAELNARVMSRFTDVFTAVRTRLSDAVDRGEVHPDIDPDRL
                     VEVIGGATMLRVLLQPGEPLGEDWITQTAAIVVHGVVA"
     gene            665..1801
                     /locus_tag="VFC01_29045"
     CDS             665..1801
                     /locus_tag="VFC01_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZN83700.1"
                     /translation="MATEPAVEDVSRLEHSSRDLSTLPDVMARWLSTVMPGGVTPEIT
                     VESGVDANGMSSETIILTGRWDEGRQRRDEKWVARVAPTAQDVPVFSSYRMDHQFDVI
                     RLVGEKTDVPVPRVRWLESTGSVLGTPFFLMDYIEGQVPPDVMPYTFGNNWFADAPAE
                     RQRELQDSTVEVIAKLHSIPEPEKTFGFLDDGSEPNALRRNLNWLQSWYQFAVPDIGR
                     SELLERAFDWLEANWPADVAAEDPVLVWGDSRIGNVLYSDFRPVAVLDWEMAVLGPRE
                     MDVAWIIFAHMVFQELAGLAGLPGLPDFMREADVKATYTQSTGVELGDLHWFYVYSGV
                     IWACVFMRTSARRVRFGEIEKPDDVESLFYHGSVLKRLIGEGEA"
     gene            1801..2916
                     /locus_tag="VFC01_29050"
     CDS             1801..2916
                     /locus_tag="VFC01_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83701.1"
                     /translation="MLGPMDEFPVHQVPQPIAWPGSSDRNFYDRSYFNAHDRTGDIFV
                     ISGIGYYPNLGVKDAFLLVRRGDKQTAVHLSGAIDQDRLNQHVGNYRVEVLEPLHKLR
                     IVLDETEGIAADLTWEGLFDVVQEQPHIMRRGNRVTLDAQRFAQVGAWSGDLLIDGEE
                     IAVDPTRWIGTRDRSWGIRPIGEPEPAGRPDDPPFEGMWWLYIPMAFDDFGIVLIIQE
                     DPHGFRSLNDCTRIWKDGRVEQLGWPRVKIHYRSGTRIPTGATIDATAFDGSPVHFDV
                     ESKLPVPIHVGGGYGGDSDWLHGMWKGDNFTERLTYHMTDPAIVGRAGFGVIDHVGRA
                     VCRDADGVEREGWGLFEHGALGRHDPSGFADWLTVAP"
     gene            3033..4226
                     /locus_tag="VFC01_29055"
     CDS             3033..4226
                     /locus_tag="VFC01_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HZN83702.1"
                     /translation="MAQKVTAMDIRAATALAGQIDNVSQFCRDRQISRQTFYKYRRRF
                     GEDGLGGLQEQSRRPNSSPGQTPAAIEETVLRQRKLLLEQGRDHGPQSIVWTLERDEM
                     TVPSRSTVWRILTRHGLITPQPQKRPKSATKRFCFSRPNECWQSDWTGWHLADGTDVA
                     IAGSIDDHSRYVPALAAGLGQGTAELVWSVMLAGITECGIPAMSLADNGMVYTGRLHA
                     FEAAFEANLRALGTHTINSTPSHPQTCGKIERFWQTLKKWLRAQPTAGTIEKLNDLLQ
                     RFRSFYNHHRPHRALRGATPAEAFTATAPARPADRPLPAPVFVSRHTVGETSGKLHVA
                     PYRVNVGLRWAGHSCDVVRQGDHIAIFSGTTLIRTLTADPTRNYQPSAPNTRTYRGRE
                     PKPPP"
BASE COUNT          760 a         1408 c         1378 g          732 t
ORIGIN      
        1 gggaactcct cgagcatcga tgtctatgcc accacaccat ggacgactat ggcggccgtc
       61 tgcgtgatcc agtcctcacc gagcggttcg ccgggctgca gcagcacgcg caacatggtg
      121 gcaccgccga tgacctcgac cagtcggtcc gggtcgatgt cggggtgcac ctccccgcgg
      181 tcgacggcgt cgctcagccg cgttcggacc gcggtaaaca cgtcggtgaa gcgtgacatc
      241 acccgcgcgt tgagttcggc gtccaccgcc atgtcggcga tcagtcccgg cattgccgcg
      301 cgcacgagag ggctcgtgaa aacgtcgcgg gcggcggcga tcatcgcgtg aatgtcggcg
      361 gcgatgtcgc ctggcggcgt ctcgatggcc gtcggcgccg cagggaaggc cgcctcgtgt
      421 accagttcgg ccttgctcga ccaccgccgg tacagcgcgg tcttggtggt tccggcgcgc
      481 tcggcgaccg cggccatggt caggttcgaa tagccgattt ccacaagcag atccgcggtg
      541 gcgcgcagta tggcagcgtc gatacgcgga tcccggggcc gccccggggt cttgtcaacc
      601 actgacgcgt ctgctttcat aacgctaccc accgtatcgt aattaccggc cgaaggaaga
      661 acttgtggct actgaaccgg ctgtcgagga tgtcagccgc ctcgagcact cgagtcgtga
      721 tctcagcacg ctgccagacg tgatggcgcg gtggctgtcc accgtgatgc caggcggcgt
      781 gacacccgag atcaccgtgg agagcggcgt cgacgccaac ggaatgtcgt cggagaccat
      841 catcctgacc ggccgctggg acgagggccg gcagcgccgc gacgagaaat gggtggcgcg
      901 ggtggcgccg accgcacagg acgtgccggt gttctcgtcg tatcggatgg atcaccagtt
      961 cgacgtcatc cggctggtcg gtgagaagac cgacgtcccg gtgccccggg tgcggtggct
     1021 ggaatcgacg ggctcggtgc tcggcacgcc gttcttcctg atggactaca tcgagggcca
     1081 ggtgccgccg gacgtgatgc cgtacacctt cggcaacaac tggttcgccg acgcccccgc
     1141 cgagcgtcag cgcgaactgc aggacagcac cgtcgaggtg atcgccaagc tgcactcgat
     1201 tcccgaaccc gagaagacgt tcgggttcct cgacgacggc tcggagccga atgcgttgcg
     1261 gcgcaatctc aattggctgc aatcgtggta tcagttcgcc gtgcccgaca tcggccgctc
     1321 tgagctgctc gagcgggcgt ttgactggct tgaggcgaac tggcccgccg acgtcgccgc
     1381 cgaggatccg gtgctggtgt ggggcgactc ccgcataggc aatgtgctgt actccgactt
     1441 ccgaccggtc gccgtgctgg actgggagat ggcggtgctc ggaccgcgtg agatggatgt
     1501 cgcgtggatc atcttcgcgc acatggtctt tcaggagctc gcgggcctgg ccggcctgcc
     1561 ggggctgccc gacttcatgc gggaagccga cgtcaaggcg acgtacacgc agtcgaccgg
     1621 tgtcgagctg ggcgacctgc actggttcta cgtgtactcc ggcgtcatct gggcgtgcgt
     1681 gttcatgcgg accagcgccc ggcgggtgcg gttcggcgag atcgagaaac ccgacgacgt
     1741 ggagtcgctg ttctatcacg gctcggtgct caagcggctc atcggcgaag gagaggcgta
     1801 atgctcggcc ccatggacga attcccggtg catcaggtcc cgcagccgat cgcgtggccg
     1861 ggctctagcg accgaaactt ttacgaccgt tcgtacttca acgcgcacga ccgcaccggc
     1921 gacatcttcg tgatctcggg catcgggtac tacccgaatc tcggcgtgaa ggacgcattc
     1981 ctgttggtgc ggcggggcga taagcagacg gcggtgcatt taagcggcgc catcgatcag
     2041 gaccgcctca accagcacgt cggcaactac cgcgtcgagg tcctcgagcc actgcacaaa
     2101 ctgcgcatcg tgcttgacga aaccgagggc atcgcagcgg atttgacgtg ggaagggctg
     2161 ttcgacgtcg tccaggaaca gccgcacatc atgcggcgcg gcaaccgagt cacgcttgac
     2221 gcgcagcgct tcgcgcaggt gggcgcgtgg agcggcgatc tgctgatcga cggcgaagag
     2281 atcgccgtcg acccgacccg ttggatcggc acccgcgacc gctcgtgggg tatccggccc
     2341 atcggcgaac ccgagcccgc aggccgtccc gacgatccgc cgttcgaggg gatgtggtgg
     2401 ctctacattc cgatggcgtt cgacgacttc ggcatcgtcc tcatcattca ggaggacccg
     2461 cacggcttcc ggtcgctcaa cgactgcacc cgcatctgga aggacggccg cgtcgagcaa
     2521 ctcgggtggc cgcgggtcaa gatccactat cgctcgggca ctcgcattcc gaccggtgcg
     2581 accatcgacg cgaccgcatt cgacggcagc cctgtgcatt tcgacgtcga gtccaagctg
     2641 cccgtgccga tccacgtcgg cggcggctac gggggtgact ccgattggct gcatgggatg
     2701 tggaagggcg acaacttcac cgaacgcctg acctaccaca tgaccgatcc ggcgatcgtc
     2761 ggtcgcgcag gcttcggcgt catcgaccac gtcggtcggg ccgtctgtcg cgatgcagac
     2821 ggcgtcgagc gcgaaggatg gggcttgttc gagcacggtg cactcggccg tcacgatccg
     2881 tccggcttcg ccgattggct gaccgtcgca ccttgagtga tttcggcgcg catatccacg
     2941 ctcagcgacg tgtcctgttt cgggacatcg ctgacagttg tatgtctcgg gacatcgctg
     3001 acacctgagt agggccgcga ccaggatggt ccatggccca gaaggtgacg gcgatggata
     3061 tccgtgccgc gacggcgtta gccgggcaga tagacaacgt ttcgcagttc tgccgtgatc
     3121 ggcagatcag tcggcagacg ttttacaaat accggcgccg attcggtgag gacggtctgg
     3181 gcgggctcca agaacagtca cggcggccaa attcttcacc cggccagacc ccggccgcga
     3241 tcgaagagac ggtactgcgt cagcggaaac tgctgcttga gcagggccgt gaccatgggc
     3301 cgcagtcgat cgtgtggacg ctagagcgcg acgaaatgac tgtgccgtcg cgctcgacgg
     3361 tgtggcggat tttgacccgc cacgggctga tcacccccca gccccagaaa cgacccaaat
     3421 cggcgaccaa acggttctgc ttctcccggc ccaatgaatg ctggcagtcc gactggaccg
     3481 gctggcacct ggccgatgga accgatgtgg ccatcgccgg cagtattgat gaccactccc
     3541 ggtatgtgcc cgcactggcc gccggccttg gacagggcac tgcggagttg gtgtggtcgg
     3601 tgatgctggc cggcatcacc gagtgtggaa ttccggcaat gtcgttggcc gacaacggaa
     3661 tggtctacac cggtcggctg cacgcgttcg aggctgcctt cgaagccaac ctgcgtgccc
     3721 tgggcacaca caccatcaac tccaccccgt cccatccgca gacctgcggc aagatcgaac
     3781 ggttctggca gacgctgaag aaatggctac gcgcccaacc cactgcgggc actatcgaga
     3841 aactcaacga cctcctgcag agatttcgca gcttctacaa ccaccaccgc ccgcaccgag
     3901 cgctgcgggg tgccacgcca gccgaagcat tcaccgccac tgcaccggcc cgacccgccg
     3961 accggccgct gcccgcaccg gttttcgtca gccgccacac cgtcggtgaa acctccggca
     4021 agctgcacgt tgcgccctac cgagtcaacg tcggcctgcg ctgggccgga cacagctgcg
     4081 acgtcgtacg ccaaggcgac cacatcgcca tcttcagcgg caccaccctg atccggacac
     4141 tcaccgccga tcccacccgc aactaccagc ccagcgcccc aaatacccgg acttaccgcg
     4201 gccgcgagcc caaaccacca ccatgagtgt cagcgatgtc ccgagacata actgtcagcg
     4261 atgtcccgag acaccaca
//
LOCUS       DASRJA010000646        12366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2341308,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000646 DASRJA010000000
VERSION     DASRJA010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12366
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341308"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..257)
                     /locus_tag="VFC01_29060"
     CDS             complement(60..257)
                     /locus_tag="VFC01_29060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83703.1"
                     /translation="MDASTNARIGVDDAVEHDPTEGSHVEGGVVEQDPIYGYQSVNVE
                     AQRDSSSSLLNWNRNAPEEAP"
     gene            515..904
                     /locus_tag="VFC01_29065"
     CDS             515..904
                     /locus_tag="VFC01_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83704.1"
                     /translation="MYSAAIDALPDPHDPEFPHRVGVILAGLRKLSGSLSEAAGRSRV
                     TPSVIVALSGVRHRYDELIDTAAHGPGATLGQRLYVARERAKLSTKEAANGVGLRKDL
                     IEAVEAEEPATEEETSKIKDLIAALGG"
     gene            complement(921..1841)
                     /locus_tag="VFC01_29070"
     CDS             complement(921..1841)
                     /locus_tag="VFC01_29070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83705.1"
                     /translation="MARSAMRFVVCAGVLTTGLLMGGGGAGIAFADTGDAAGGAPEPA
                     AVTSDVPENNSDSGATGASHPDPPTSTVGNGREDVGVQSTVDGKDDEDDKKESASPGA
                     TTKKVKGSVTIPFLPISIPIPTFCDFLAAMQYQPQPTPAPGPVFKTQEEAPPVADSGG
                     GGIDPLSVGVAAEPPVLRAPMVIAPAAVPRAPAPPPVAPRGEAAGAGPTPVAATDVAA
                     GAAAPVIRGSVQPTVEPAATTLTSMSGQATRLGYPRSLRNPTAAELAVLALPGTAGLL
                     LLTLSGGVIGYRQANTARFIRTRSAARFLR"
     gene            1995..2795
                     /locus_tag="VFC01_29075"
     CDS             1995..2795
                     /locus_tag="VFC01_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HZN83706.1"
                     /translation="MTSRDHGDPGDAPSAPPPLTDVENAVRPSAAEEARTIAASTNTG
                     TLATLTTDGDPWASFVTYGLLGGVPVLCVSNLAEHGRNLAADPRASIAIVAPQREADP
                     LASGRVTLAGVVEEPAGEERAAAREAHLAGVAAAKYYIDYSDFSLWVLRVRRVRWVGG
                     YGRMDSATGEAYAAASPDPVSPRSDGAIAHLNGDHADALVAMAQALGGYPDTTEATCT
                     GADRYGLDLRIVTPRGVAYTRIGYAAPIDSIDELRSATVELTRRARQA"
     gene            2810..3385
                     /locus_tag="VFC01_29080"
     CDS             2810..3385
                     /locus_tag="VFC01_29080"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZN83707.1"
                     /translation="MSATDRPDSWQAAFDLIRTRGYEHREEPFKLASGQLSHDYIDGK
                     FAVDTGERLAIVSRAVADLAARHGIVFDAVGGLTMGADPLAHGVAMVTGKAWFSVRKE
                     QKQRGREQWIEGTRIEPGMRVMLVDDVISTGGSTEKAYERVTAVGAVVTGVIPMVDRG
                     DVATKRFAARGVPFVALVTYRDLGIEPVKDV"
     gene            3467..4834
                     /gene="lysA"
                     /locus_tag="VFC01_29085"
     CDS             3467..4834
                     /gene="lysA"
                     /locus_tag="VFC01_29085"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZN83708.1"
                     /translation="MTLLDYVPSLHKAATSRIDPAIWPLTAGVDDLGRLCVGDVALTD
                     IADEFHTPTYVLDEADFRYRLQQYRGALRGVEILYAAKSLLTTDVARWVVEEGIGLDV
                     SSAGELAIALAGGVDPSRIILHGNAKPVDELAAAATVGIGRIVVDSAMEIAYLSCVAR
                     RPQPVLIRVTPGIDIGGHPAVVTGIDDQKFGFPRTDGRALAAAQRILHQPALELVGLH
                     CHIGSQVTDATIYGEVIRRMIATMADIRRSHGVVLHELNIGGGHGVPYVSGDPALNLD
                     ELADIIDDALDWACAAERFPRPRIVVEPGRAISARAGVTLYRVVSVKSQPGGRTFVSV
                     DGGMSDNPRVALYGAKYTVTLANRHALGPTQRVTVAGRHCESGDEIARDVELPADVHP
                     GDLLAVACTGAYQHSMASNYNMVGRPPIVAVKGGRVTELVRRETTADLLARDRGWSGG
                     VVLHD"
     gene            complement(4808..5854)
                     /locus_tag="VFC01_29090"
     CDS             complement(4808..5854)
                     /locus_tag="VFC01_29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN83709.1"
                     /translation="MTTGVAVELNELTRLYGSVKALDGLSLHIEPGELVALLGPSGCG
                     KTTALRILAGLDEATSGTVSVGGKDVSNVPANKRDMGMVFQAYSLFPHLTVLDNVAFG
                     LKMRGKAKSERMSQAADMLDLVGLSAHKHKYASELSGGQQQRVALARALAIQPRVLLL
                     DEPLSALDAKVRAQLRDEIRRVQLEVGTTTLFVTHDQEEALAVADRVGVMSQGRLEQL
                     AAPADLYANPATPFVAEFVGLNNKVPAQVSGGQAHLLGTSVPALEGSIAGPGLAMVRP
                     ESVTIVADSAGTSTISSVAFLGPISRVYAALADGSVISAQMASSQAKAFSPGDPVTVG
                     VEPTGVLVVKDDAA"
     gene            complement(5851..6624)
                     /locus_tag="VFC01_29095"
     CDS             complement(5851..6624)
                     /locus_tag="VFC01_29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZN83710.1"
                     /translation="MLWVAFGLFFLFPLYAMADFSTRNLIAGGRTMQAWQNLIADEAL
                     YQSIVISLLLAGFTVVAMLLVLVPTMIWVRLRTPWAKGMVEFLCLLPLTIPALVIVVG
                     LRNVYLWVTYFLGESALTLTFVYVVLVLPFAYRAIDAALAAIDLKTLAEAARSLGAGW
                     ATTILRVVVPNIWSGILSAAFISIAVVLGEYTIASLSGYQTLQVQIVQIGKTDGPTSV
                     AASLAVLLFGFVLLLVLSLLTRGRRRATVENVVQPGVAA"
     gene            complement(6660..7505)
                     /locus_tag="VFC01_29100"
     CDS             complement(6660..7505)
                     /locus_tag="VFC01_29100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZN83711.1"
                     /translation="MLRRLRDALPLLPFLAVVVIFLIIPTVTVIVSAFFADGAFSLAR
                     IEALFSGTALAALWKSVLLSASTALIGAFLGAVLAWLIVSNPPTSMVRRAVISLCSVL
                     AQFGGVALAFAFLATIGLNGVLTLWVQQVFGWNLAGSGWLYSLTGLILVYTYFQIPLM
                     VIVFVPALEGLREQWREAAVSLGASTWRYWREVAVPLLTPAFLGSALLLFANAFAAYA
                     TAAALVSQGSPILPLLIRAALTSEVVLGQSGFAYALALEMIIVVAVVMVAYNALVRRT
                     ARWLR"
     gene            complement(7507..8634)
                     /locus_tag="VFC01_29105"
     CDS             complement(7507..8634)
                     /locus_tag="VFC01_29105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN83712.1"
                     /translation="MKRSLLAVSAASALVLAGLIACAPPEKKSEGEKTQSGVNASEAK
                     SAADFGGMDALVEAAKREGELNVIALPPDWANYGAIIKAFSDTYGIKVNSAQPDAASQ
                     DEINAANQQKGKSTAPDVFDLGQSVALANTAMFAPYTVATWDDIPAAYKDPNGAWVND
                     YGGYMSIGFDSSKVPPVTGVDDLLKPEYKGKVALNGDPTQAGSAFSGVLMVALSQGGS
                     ADDIAPGVDFFRKLKAAGSFLPVDPTPATIESGQTPVVIDWNYLNAAVTAKLPSWQVV
                     VPPNAVVAGYYYQAINKDAPHPAAARLWQEFLYGDQGQNLLAAGGVRPVRADQMLQAG
                     TIDKAVVNKIPVIDGPVTVPSPDQTEKATKYLADNWAGAIG"
     gene            8830..9474
                     /locus_tag="VFC01_29110"
     CDS             8830..9474
                     /locus_tag="VFC01_29110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83713.1"
                     /translation="MAEREASGDFDYLWDDPSDVDGVTSDDQDFQAQPAEELDPRLND
                     PGDDQWDDPDDNFWDNQPEVVDPGHFDAFDDNTWRFTPAPPPWYRTKQAVTAIIAASA
                     ALAAIVVSGVLLVFRSPGDTVNQVTSSVTPTAPTSVAPIQVASSEAPPPPPSPPPEPP
                     PETAASPVSPAPSPNPAYEPRPAKPPEIGVTRTPVTRQPISVAPQPRGSANMRR"
     gene            complement(9488..10135)
                     /locus_tag="VFC01_29115"
     CDS             complement(9488..10135)
                     /locus_tag="VFC01_29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZN83714.1"
                     /translation="MANLLHIDSSVQGDQSVSRRLTARAAERWRVTHPGGTVTYRDLA
                     VNPIPHLDAETGLARMVPAEQRTPEQDASFALSVELIDEIKRADTVLLGLPLYNFGPP
                     STIKAWIDHIVAPGLSIDTETGGGLLSGTDFVVLASRGGGYGPGTPREGWDHNQTWLP
                     HAVSLTGLQPRFIIAELTMAEVNPAMAELKPLAAESMNGALADVEKLWAASESAA"
     gene            10210..10704
                     /locus_tag="VFC01_29120"
     CDS             10210..10704
                     /locus_tag="VFC01_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZN83715.1"
                     /translation="MADLPIVQPAEHLSAPLLVHLARRMQTEAEVELAAFGLRTRHVI
                     ALTLLRDLGEQNQSDLPATLGMDPTNVVALLNELEADDLVQRRRSAQDRRRHTVSLTA
                     AGKARLKDIEDLLTGVEQRVLDPLSPEEQQTLYALLSRATASPTCAEALDTTRACMEG
                     QHQE"
     gene            10714..11121
                     /locus_tag="VFC01_29125"
     CDS             10714..11121
                     /locus_tag="VFC01_29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HZN83716.1"
                     /translation="MAEFDAVAMFAESPVAMLATVGADGAPHVVPVVFAVHGDVVYTA
                     VDAKRKSTKKLRRLANIEANPHVSLLVDHYDDDWSQLWWVRADGEAAVHYSGEEMAGG
                     YAWLRQKYLQYQRIALDGPVVTVDVKKWSSWQA"
     gene            complement(11128..11493)
                     /locus_tag="VFC01_29130"
     CDS             complement(11128..11493)
                     /locus_tag="VFC01_29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005514077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN83717.1"
                     /translation="MTSYNELAQRYIDTWNETDPSARRDAVDRLYTEDARYVDPLAVA
                     EGREAITSMIGAVQQQFAGFSFRLAGPVDGHHNQARFGWELGPAGSPAPIVGFDVAVS
                     DDSGRIQTVLGFLDKVPTG"
     gene            complement(11553..12125)
                     /locus_tag="VFC01_29135"
     CDS             complement(11553..12125)
                     /locus_tag="VFC01_29135"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HZN83718.1"
                     /translation="MPSVGMRIATLLAAVIMTAVVSSCGVPSPTPESQSTKHNAADVT
                     FAQKMIPHHQQALDMAAMVPSHTTNHELIVMAKHIALDQQAQVDTLQGLLRQWDEPAA
                     TDRMGHDGMGMDGMVDAATMGKLPTLKGADFDNLWLRSMISHHEGAVAMAEPEVAQGE
                     NPTTVKMAKIIVEWQQFEIGQMHAMLGPSE"
     gene            12186..>12366
                     /locus_tag="VFC01_29140"
     CDS             12186..>12366
                     /locus_tag="VFC01_29140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83719.1"
                     /translation="MDEPRSMSNVKPPTPSKTRSPAPSVIGTTCRRSSSTAPRDSYWL
                     SVAAPPAIATCRSPAA"
BASE COUNT         2115 a         4109 c         4087 g         2055 t
ORIGIN      
        1 gccggcgtac cagcgctgct cgggcagcca ctcggaccac ggcaggtttg cagactggct
       61 catggcgcct cctccggcgc atttcggttc caattcagca gcgacgacga actgtcgcgc
      121 tgcgcttcga cattcaccga ctggtagccg tagatcgggt cctgctcgac cacgccgccc
      181 tcgacgtggc tgccctcggt ggggtcatgt tcgactgcat cgtcgacgcc gatccgcgca
      241 ttcgtcgaag catccatcga ccttcaaacc tacccgccca tcgggaaatt gaatctgctc
      301 agcgtgtcgc caacgcatgg agcaaagtag tggatccgat ggtgtaaagg aacggttgaa
      361 acctcgcgga accgggatac cgtttgcgcg accatgtccc attggcgggt ggcattggtc
      421 gccattaatt cttcgacgaa tcgcgtggcc cttgtctgag ccgaatgatg gtgaagctcc
      481 agtaatcgtt ggtatggccg atgcggctat gcatatgtac tccgccgcaa tcgacgcgct
      541 tcccgacccg cacgatcccg agtttcccca ccgagtgggc gtcatcctcg ccgggctccg
      601 caaactctcg ggctcattgt ccgaagcggc gggccgcagc cgggtcaccc cgtcggtcat
      661 cgtcgccctc agtggtgtgc ggcaccgcta cgacgaactg atcgacaccg cggcgcacgg
      721 gccgggcgcg acgctgggtc agcggctgta cgtcgcccgc gaacgcgcca agctgtcgac
      781 caaggaggcg gccaacgggg tgggtctgcg caaggacctg atcgaggcgg tcgaggccga
      841 agagcccgcc accgaagaag agaccagcaa gatcaaggac ctgatcgccg ccctcggcgg
      901 ctagcgtcgc aggaacccgc tcagcgtagg aagcgcgcag cgctccgcgt gcggatgaag
      961 cgcgcggtgt tggcctgtcg gtagccgatg actccgccgg acagggtgag aagcagcagt
     1021 ccggccgtcc ccggcaacgc gaggaccgcg agctcggctg cggttggatt gcgcagcgag
     1081 cgcgggtacc cgagccgggt ggcctgtccg ctcatcgatg tcagggtcgt ggccgccggt
     1141 tccacggtcg gctgaacgga accgcggatc accggcgccg cggctccagc ggcgacatcg
     1201 gtggcagcga ccggcgtcgg gcctgcgcct gccgcctcac cgcgcggggc aacgggtggc
     1261 ggtgctgggg cacgcgggac ggccgcaggc gcgatcacca taggggcccg taacaccggg
     1321 ggctcggccg cgacgccgac ggacagcggg tcgatgccac cgcctccgga gtcggccacc
     1381 ggcggcgcct cctcttgcgt cttgaacacg ggacctggcg caggggttgg ttgcggctgg
     1441 tattgcatcg cggcgaggaa gtcgcagaac gtcgggatcg ggatggaaat gggcaggaac
     1501 ggaatcgtca ccgacccttt gacctttttc gtcgtcgcgc cgggagaggc gctttccttt
     1561 ttgtcgtctt cgtcgtcttt gccgtcgacg gtcgattgga cgccgacatc ctcgcggccg
     1621 ttgccgacgg tgctggtggg cgggtcggga tggctggcgc cagttgcgcc ggaatccgaa
     1681 ttgttctcgg ggacatcact ggtcaccgcg gcgggctccg gtgcgccacc tgctgcatca
     1741 ccggtgtctg cgaacgcgat gcctgcgccc ccgccgccca tcaggagacc ggtggtgagc
     1801 acgccggcac atacgacgaa tcgcattgcc gaacgcgcca tgacgtcctc actccgaaga
     1861 gaatattgcc cgttaaaaca cttaagtctg cgcaattctc gcatattcag ggcaaccacc
     1921 cggacgaaag attggtgccg aattccacga cccacgtcat tcttcatccg cgacccgttc
     1981 ggtatcgtgc cgacgtgacg tcccgtgatc acggcgaccc gggcgatgcc ccgtcggccc
     2041 ctcccccgct taccgatgtc gaaaacgcgg ttcgtccgtc ggccgccgaa gaagcccgca
     2101 cgatcgcggc gtcgaccaat acgggcacac tggcgacact gaccaccgac ggtgatccgt
     2161 gggcgtcgtt cgtcacgtat ggactgctcg gcggtgtacc ggtgctctgc gtatcgaatt
     2221 tggccgagca cggccgaaac ctcgcggccg acccgcgcgc cagcattgcg atcgtggcgc
     2281 cgcagcggga agccgatccg ctggccagcg ggcgcgtcac attggcaggc gtcgtcgagg
     2341 aaccggccgg cgaggagcgc gcggccgccc gcgaggcgca cctggccggc gtcgccgcgg
     2401 cgaagtacta catcgactac agcgacttct cgctgtgggt gctgcgggtg cgacgggtgc
     2461 gctgggtcgg cgggtacggg cggatggact cggcgaccgg cgaggcctac gccgccgcgt
     2521 caccggaccc tgtgtcgccg cgatcggatg gagcgatcgc tcatctcaac ggtgaccacg
     2581 ccgacgcgct ggtcgcgatg gcccaggcac tcggcggata tcccgacacc accgaggcca
     2641 cgtgcaccgg cgccgaccgg tacggactcg acctgcggat cgtcacgccg cgcggcgtgg
     2701 catacacccg gatcggctac gccgcaccga tcgattcgat tgacgaattg cgttcagcga
     2761 cggtcgagtt gacgcgtcgg gcgcgccagg cgtgaaatac gatcgcgata tgtctgcaac
     2821 cgaccgcccg gacagctggc aagcagcgtt cgatctgatt cgcacccggg gatacgagca
     2881 tcgcgaagag ccgttcaagt tggccagcgg gcagctcagc cacgactaca tcgacggcaa
     2941 attcgcggtc gacaccgggg agcggctggc gatcgtcagc cgtgcggtcg ccgacctcgc
     3001 cgcccggcac gggatcgtgt tcgacgctgt cggcggcctc accatgggtg cggacccgct
     3061 ggcgcacggt gtcgcgatgg tcaccggcaa ggcctggttc tcggtgcgca aggagcagaa
     3121 gcaacgcggc cgcgagcagt ggatcgaagg cacccggatc gagcccggga tgcgggtgat
     3181 gctcgtcgac gacgtgatca gcaccggggg atcgaccgag aaggcgtacg agcgcgtcac
     3241 ggcggtcggc gccgtcgtca ccggggtgat cccgatggtg gaccgcggcg acgtcgccac
     3301 gaaacgcttc gcggcgcggg gcgtgccgtt cgtcgcgctg gtcacgtatc gcgatctggg
     3361 gatcgaacct gtcaaagacg tctgagcgca tcaatattcg gtgctgacgc gtcaaaaccg
     3421 cgtcaagatc cgtgctcctg gcgtccagcg ggcgcacggt ggctgtatga cattgctgga
     3481 ctacgtgccg tcgctacaca aggccgccac gagccggatt gatcctgcga tttggccgct
     3541 gaccgcgggc gtcgacgacc tgggtcggct ctgcgtcgga gacgtcgcgt tgacggatat
     3601 cgccgacgag ttccacaccc ccacatatgt tctcgatgag gccgacttca ggtatcggct
     3661 gcagcagtac cgcggcgcct tacggggtgt cgagatcctc tacgccgcca agtcgttgct
     3721 gaccaccgac gtcgcgcggt gggtcgttga ggagggcata ggcctcgacg tcagctccgc
     3781 cggcgagctt gcgatcgcgt tggccggcgg tgtggatccg agccgaatca tcctgcacgg
     3841 caacgcgaaa ccggtcgatg aattggccgc cgccgctacc gtcggcatcg ggcgcattgt
     3901 cgtggattct gcgatggaga tcgcctatct gtcgtgcgtg gcccggcggc cgcaaccggt
     3961 gctgatccga gtgacgccgg gcatcgacat cggcggccac cctgcggtgg tgacgggcat
     4021 tgacgaccag aagttcggat tcccccgcac tgacgggcgc gctctcgcgg ccgcacaacg
     4081 catcctccac caaccggcac tcgaattggt tggcctgcat tgccatatcg gatctcaagt
     4141 caccgatgca acgatctacg gtgaggttat tcggcggatg atcgccacta tggccgatat
     4201 ccggcgcagc cacggcgtgg tcttgcacga gctcaatatc ggtggcggac atggcgtgcc
     4261 gtacgtcagc ggcgatccgg cgctcaatct ggacgaactg gcggacatca tcgacgacgc
     4321 gctcgattgg gcgtgtgcgg cagagcggtt cccgcggccg aggattgtgg tcgagccggg
     4381 gcgtgcgatc agcgcccgtg ctggtgtgac tctgtaccgg gtggtttcgg tgaaatcgca
     4441 gccgggcggt cgtacgtttg tttcggtgga tggcggcatg tcggacaacc cgcgtgtggc
     4501 gctgtatggc gcgaaataca cggtgacgct ggctaatcgg cacgcgcttg ggccgacgca
     4561 gcgggtgacg gtggcgggcc ggcactgcga atccggtgac gagatcgccc gcgatgtcga
     4621 gttgcccgcc gacgttcatc ccggcgatct gctcgcggta gcgtgcaccg gggcgtacca
     4681 gcacagtatg gcgtcgaact acaacatggt cggcaggccg ccgatcgtcg ccgtcaaggg
     4741 cggacgggtg accgaactcg tgcgccgcga gacgacggcg gacctgttgg cccgcgaccg
     4801 cggctggtca ggcggcgtcg tccttcacga ctagcacgcc ggtcggctcg accccgaccg
     4861 tcaccggatc gcccggcgag aaggctttgg cctgcgagct ggccatctgg gcactgatca
     4921 ccgagccgtc cgccagcgcg gcgtataccc gggagatcgg tccgaggaac gccaccgagc
     4981 tgatcgtcga cgtgccagcg gaatcggcga cgatggtgac cgactccggg cgcaccatcg
     5041 ccaggcccgg tcctgcgatc gacccctcca acgccggcac cgacgtaccc agcagatggg
     5101 cctggccacc cgaaacctgc gctggcactt tgttgttcag gccgacgaac tcggcgacga
     5161 acggcgtcgc gggattggcg tagaggtcgg ccggggccgc cagctgctcg agcctgccct
     5221 ggctcatcac gccgacccgg tccgcgacgg cgagggcctc ctcctggtca tgggtgacga
     5281 acagcgtcgt cgtgccgact tccagctgga cgcgccgtat ctcgtcgcgc agctgggcgc
     5341 gcaccttcgc gtccagcgcc gagagcggct cgtccagcag cagcacccgc ggctggatcg
     5401 ccagcgccct ggccagcgcg acacgctgct gctgtccgcc ggagagctcg ctggcgtact
     5461 tgtgcttgtg tgccgacagc ccgaccagat ccagcatgtc ggcggcctga gacatgcgct
     5521 cgctcttggc ttttccacgc atcttcaatc cgaaggcgac gttgtcgagc actgtcagat
     5581 gcggaaacag gctgtaggcc tggaacacca tgcccatgtc gcgtttgttg gcgggcacat
     5641 tgctgacgtc cttgccaccg accgacaccg tgcccgacgt cgcttcatcg aggccggcga
     5701 ggattcgcag cgccgtggtc ttgccgcacc cggacgggcc gagcagcgcg acgagctcgc
     5761 ccggctcgat gtgcaagctg agcccgtcca gtgccttgac gctgccatac aggcgggtca
     5821 actcattgag ttcgacggcg actccagttg tcatgcggcg actccaggtt gaacgacgtt
     5881 ctcgacagtg gcacggcggc ggccgcgcgt cagcagcgac agcacgagca gcagcacgaa
     5941 gccgaacagc agcacagcca gcgacgcggc caccgacgtc gggccgtccg tcttgccgat
     6001 ctggacgatc tgaacctgca gcgtctggta accgctcagc gacgcgatcg tgtactcacc
     6061 cagcacgaca gcgatggaaa tgaaagcggc cgagaggatt ccggaccaga tgttgggcac
     6121 cacgacccgc aggatcgtcg tcgcccagcc ggcacccaac gagcgcgcgg cctctgcgag
     6181 cgtcttcagg tcgatggctg ccagcgcagc gtcgatcgcg cgatatgcga atggcaacac
     6241 cagcactacg tagacgaacg tcagcgtcaa cgccgattca ccgaggaagt acgtcaccca
     6301 tagatagacg ttgcgcagcc cgaccacgat cacgagcgcc gggatcgtca ggggaagcag
     6361 acacagaaac tccaccatgc ccttggccca cggcgtccgt agccgcaccc agatcatcgt
     6421 cggcaccagc accagcagca tggccacgac cgtgaaaccc gccagcagca acgagataac
     6481 gatcgactgg tacaacgctt cgtctgcgat caggttctgc cacgcctgca tggtgcgtcc
     6541 gccggcgatc aggttgcggg tggagaagtc ggccatcgcg tacagcggga acaagaagaa
     6601 cagcccgaac gccacccaaa gcatagcgcg aaagaccttg ccgcccagcg ttctccgcct
     6661 caccgcagcc acctcgccgt gcgtcggacc agtgcgttgt aggccaccat caccacggcc
     6721 acaacgatga tcatctctag cgccaacgcg tacgcgaacc cggactggcc gagcaccacc
     6781 tcgctggtca gcgcggcccg gatcagtagc ggcaggatcg ggctgccctg gctgaccagc
     6841 gcagcggcgg tcgcatatgc cgcaaaagcg ttggcgaaca acaggagcgc cgagcccagg
     6901 aacgccggcg tgagtagcgg gaccgcaact tcgcgccagt agcgccacgt cgacgccccg
     6961 aggctgaccg ccgcctcgcg ccactgctct cgcagtcctt cgagtgcggg tacgaacacg
     7021 atcaccatca gcgggatctg gaagtacgtg tagaccagga tcagcccggt caggctgtac
     7081 agccagccgg agcccgccag gttccatccg aacacctgct gcacccacag cgtcagcacg
     7141 ccgttgagtc cgatcgtggc caggaatgcg aatgccagtg cgacgccgcc gaactgagcc
     7201 aagacgctgc acagtgagat caccgcgcgg cgcaccatcg aggtgggcgg attgctcacg
     7261 atcagccacg ccagcaccgc gccgaggaac gccccgatca gggccgtgct ggccgaaagc
     7321 agcacactct tccacagcgc cgccagcgcc gtgccggaga acagcgcctc gatgcgcgcc
     7381 agcgaaaacg cgccgtcggc gaagaacgcg ctgacgatca ccgtcaccgt agggatgatc
     7441 aggaagatca ccacaacagc gaggaacggc agcagcggca atgcgtcccg taaccgtcgc
     7501 aacacgtcag ccgatcgccc cggcccagtt gtccgccaga tacttggtgg ccttttcggt
     7561 ctggtccgga gacgggacgg tgacggggcc gtcgatgacc gggatcttgt tcaccacggc
     7621 cttgtcgatc gtgcccgcct gcaacatctg gtcggcccgc acgggccgta ccccgcccgc
     7681 ggccaacagg ttctggcctt ggtcgccgta gaggaattcc tgccacagcc gcgcggccgc
     7741 ggggtgcggc gcgtccttgt tgatcgcttg gtagtagtaa ccggcgacca cagcgttcgg
     7801 cgggacgaca acctgccagc tcggcagctt cgcggtcacg gcggcattca ggtagttcca
     7861 gtcgatcacc accggagtct gaccggactc gatggtcgcc ggggtcgggt cgaccggaag
     7921 gaagctgccc gccgccttga gcttgcggaa gaagtcgacg cccggtgcga tgtcgtcggc
     7981 cgaaccaccc tgtgacagcg cgaccatcag caccccggag aacgccgacc cggcctgcgt
     8041 gggatcgccg ttcagcgcga ctttgccctt gtattcgggc ttgagcagat cgtcgacacc
     8101 cgtgaccggc ggcaccttgg agctgtcgaa tccgatcgac atgtagccgc cgtagtcgtt
     8161 gacccaggcg ccgttcgggt ccttgtacgc cgccgggatg tcgtcccacg tcgccaccgt
     8221 gtaaggcgcg aacatcgccg tgttggcgag cgccaccgac tggcccagat cgaagacatc
     8281 gggcgcggtg ctcttgccct tctgctggtt ggcggcgttg atctcatcct ggctggcggc
     8341 atcgggctgc gccgagttga ccttgatgcc gtatgtgtcc gagaacgcct tgatgatcgc
     8401 gccgtagttg gcccaatccg gcggcagggc aatcacattg agctcgccct ccctcttggc
     8461 ggcctccacg agggcgtcca tcccgccgaa gtcggcggcc gattttgcct cgctggcgtt
     8521 gactccggac tgcgtctttt caccctcgct cttcttctcc ggcggggcgc aggcgatcag
     8581 gcctgcaagg acgagggcgg acgcggcgga aactgccagg agagaccttt tcattggcga
     8641 aaccttccga tgggtaagag acggcgcggt ggctctgggg ttacgccgcc gggtctctgc
     8701 ggtgaatgaa tccaacgggg cgaccgaacc ctaccgtgac gggcggcgat gtgggtgcga
     8761 gttcttcgga gtcggaccgg attacggctg gccgcgcatt tgccctggta cgagcgctag
     8821 catcactgtg tggctgaacg cgaagcctcg ggggacttcg actacctctg ggacgacccg
     8881 tccgacgtgg acggggtgac cagcgatgac caggacttcc aggcccagcc ggcggaggaa
     8941 ctggaccctc ggctgaacga cccgggtgac gatcagtggg atgacccgga cgacaacttc
     9001 tgggataacc aaccggaggt cgttgacccc ggccacttcg acgcgttcga cgacaacacg
     9061 tggcgattca cgcctgcgcc cccgccgtgg tatcgcacca agcaggccgt gaccgcgatc
     9121 atcgccgcgt ccgccgcgct ggcggcgatc gtggtgtcag gggtgctgct ggtgttccgc
     9181 agcccgggcg acaccgtcaa ccaagtgacc tcgtcagtca ccccgacagc gccgaccagc
     9241 gtcgcgccga ttcaggtggc gagcagcgag gcgccgccac caccaccgtc gccgccgccc
     9301 gaaccgccgc cggagacggc ggcctcgccg gtaagccccg ccccgagccc caacccggcg
     9361 tatgagccgc ggcccgccaa gccgccggag atcggcgtga cgcgcacccc ggtgacgcgc
     9421 cagccgatca gcgttgcgcc acagccgcgc ggctccgcaa atatgcggcg ctaagctgcg
     9481 cggctagcta ggctgcgctt tcacttgccg cccatagctt ttcgacgtct gccagtgccc
     9541 cgttcatgct ttccgcggcg agcggcttga gctcggccat cgccggattc acctcggcca
     9601 tcgtcagctc ggcgatgatg aatcgcggtt gcagaccggt cagcgacacc gcatgcggca
     9661 gccaggtttg gttgtggtcc cagccttctc gaggtgtgcc gggcccatag ccaccgccgc
     9721 ggctggcgag cacgacgaag tcggtgccgc tcagcagccc gccaccggtt tcggtgtcga
     9781 tcgacagccc cggtgcgacg atgtggtcga tccacgcctt gatcgtgctg ggcggcccga
     9841 agttgtacag cggaagaccg agcagcaccg tgtcggcgcg cttgatttcg tcgatcaatt
     9901 ccacgctcag cgcgaacgac gcgtcctgct cgggcgtgcg ctgctcggcg ggcaccatgc
     9961 gggccagccc ggtctcggcg tcgaggtgcg gaatcgggtt cacggcgagg tcgcggtagg
    10021 tgaccgtgcc gccggggtgc gtcacgcgcc agcgctccgc cgcgcgggcg gtgagccgcc
    10081 ggctgacgga ctgatcgccc tggacggacg agtcgatgtg aagcaggttg gccatcttta
    10141 gtcctccata gatagtttgt gtggcactaa ctatctatag cagacggttt attccagtaa
    10201 cctgggtagg tggctgacct gccgattgtc cagccggcgg agcatctgtc cgccccgctg
    10261 ctcgtccacc tggcccgtcg tatgcagacc gaggccgagg tcgagctcgc ggcgttcggc
    10321 ctgcggactc ggcacgtcat tgcgctcacc ctgctgcgcg atctcggcga gcagaaccag
    10381 tcggacctcc ccgcgacgct gggcatggac cccaccaatg tggtcgcact gctgaacgag
    10441 ttggaggccg acgatctggt gcagcgacgc cgatcggcgc aggatcgtcg gcgccacacc
    10501 gtgtctctga ccgccgcagg caaggcacgc ctgaaggaca tcgaggacct tctcaccggc
    10561 gtcgagcaac gggtgctgga tccgctgtca cccgaggagc agcagacgtt gtacgcgctg
    10621 ctgtcgcgcg cgaccgcgag ccccacatgc gcggaggccc tcgacaccac tcgggcgtgc
    10681 atggagggcc agcaccagga atagtctgga gcaatggccg aattcgatgc cgtggccatg
    10741 ttcgcggaat cgcccgtggc gatgctcgcc accgtcgggg ccgacggtgc gccgcacgtc
    10801 gtccccgtcg tgttcgcagt acacggcgac gtggtgtaca ccgccgtcga cgccaaacga
    10861 aagtccacca agaaactgcg ccgactggcc aacatcgaag ccaatccaca cgtgagcctg
    10921 ctcgtcgatc actacgacga tgactggtcg cagctgtggt gggtgcgggc cgacggcgaa
    10981 gcggcggtcc actacagcgg cgaggagatg gccggcggct acgcgtggct gcgtcagaaa
    11041 taccttcagt accaacgaat tgcgctcgat gggccggtcg tcaccgtcga cgtgaagaag
    11101 tggtcgtcct ggcaggcgta gttttgctca gccggtcggc accttgtcga ggaatcccag
    11161 cacagtctgg atgcggcccg agtcgtcgct gaccgcgacg tcgaagccga caatcggtgc
    11221 cggtgaccct gcggggccga gctcccagcc gaaccgggct tggttgtggt gcccgtcgac
    11281 gggcccggcg agccggaagc tgaagccagc aaactgttgt tggaccgccc cgatcatcga
    11341 cgtgatcgcc tcgcgtccct cggcgacggc tagcggatcg acgtagcggg cgtcctcggt
    11401 gtagagccga tccacggcgt cgcggcgggc gctcggatcg gtttcgttcc aggtgtcgat
    11461 gtagcgctgt gcgagttcgt tataggaagt catggctctc accctcgcgt tcggaatgga
    11521 cgtctgtcga ttacccggcg ggtagcgcaa cgtcactccg acggtcccaa catggcgtgc
    11581 atctggccga tctcgaactg ttgccattcg acgatgatct tggccatctt gactgtcgtc
    11641 ggattctcgc cctgcgcgac ctcgggttcg gccatcgcga ccgcaccctc gtgatgactg
    11701 atcatcgatc gcagccacag attgtcgaaa tcggcaccct tcagcgtggg cagcttgccc
    11761 atcgtcgccg cgtcgaccat gccgtccatt cccatcccgt cgtgtcccat gcgatccgtg
    11821 gcagccggtt cgtcccactg ccgaagcagt ccctgcaacg tgtcgacctg agcctgctga
    11881 tccagagcaa tgtgtttggc catgacgatg agttcatggt tcgtggtgtg ggacggcacc
    11941 atcgcggcca tgtcgagtgc ctgctgatga tgcgggatca tcttctgcgc gaacgtgacg
    12001 tctgccgcgt tgtgcttcgt cgactgtgac tccggcgttg gcgaaggtac cccacatgaa
    12061 ctcacgacgg cggtcataat taccgctgca aggagagtgg caatgcgcat cccgacggac
    12121 ggcatgaacc cagcctaagg actaccgtcc cgctgggcgc aacgtcacgt gcacgacgtg
    12181 ttcggttgga cgaaccccga tcgatgtcca acgtcaagcc gccgacgccg tcgaagacac
    12241 gttcgccggc gccgagtgtg atcggcacga cgtgcaggcg cagctcgtcg accgcgcccc
    12301 gcgacagtta ctggttgagc gtggccgcac caccggcgat cgccacctgt cggtcgcccg
    12361 cggccg
//
LOCUS       DASRJA010000647         6799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4033178,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000647 DASRJA010000000
VERSION     DASRJA010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4033178"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            478..702
                     /locus_tag="VFC01_29145"
                     /pseudo
     CDS             478..702
                     /locus_tag="VFC01_29145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031349784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(905..1240)
                     /locus_tag="VFC01_29150"
     CDS             complement(905..1240)
                     /locus_tag="VFC01_29150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83720.1"
                     /translation="MNRPAAPQAAAASNACPDCRAAAVRLGGISGWVCHSHPGKPKRW
                     IIRRYFGRFNPSRSNHWVFGDIASGAYLPLMAWTPIVRYRKVTGGYPLIIRADRLLEP
                     MARQKTAAA"
     gene            complement(1383..2525)
                     /locus_tag="VFC01_29155"
     CDS             complement(1383..2525)
                     /locus_tag="VFC01_29155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83721.1"
                     /translation="MANPLKRGRGFQAKGPAQDLLDSMRFEEFTKAFGDSFVRGLQTG
                     GEFYAVIRITSVSSESQSKLAASLQAEANGLVAGGSFKAEFNKSNQSESTRSEYTATM
                     YQKAGTGAEISPTVTIDEVLRRYKEFPTIAKNSPGAYEIEVATYDTIPLPLPTPEEQD
                     AFVAALADARTKKLYYIQTRNDLDFARQNPEFFEDLPPAATLTAASSVYTQLINAVTR
                     HAIELSRGHLDPPTFFDPSALSPLLAEPDPIEMRRKVPDLIDVVSLVGLRGHTIAKVR
                     EVPTRTYAQFVAWATGGSDDAYEVETRGVVPSPDQYRFIQSGVDLIGGNLGGGLGGFW
                     SWIYEQDPTSGRIGPGTDVRLTTRLVVGGGMPDDLKHLWGPDRIHL"
     gene            complement(2931..3737)
                     /locus_tag="VFC01_29160"
     CDS             complement(2931..3737)
                     /locus_tag="VFC01_29160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83722.1"
                     /translation="MTEVSAEQRQKAKTAAANAAVVANDTAVVLGGAGIVVAAAGSAT
                     GLGAPAGLLVGGILGVSSFAAWAIGNRYQRLANDPPRDDFDQVTISDAQLLEEAIQAD
                     EPQATFIRYAANQLIVADTMAALVTSLERYDGAVEAGNPDSASTQADAIQSNANLVVS
                     AHTALILLATSVNDAWSTNVGDLYTSDVSFEEVPAFLADITVAGSAGLDAVFACVTCL
                     VDGDPFADLDPSNHPLISNGELPPTSELISSDVNDATAELSDPLLSIVAF"
     gene            complement(4077..5513)
                     /gene="dctP"
                     /locus_tag="VFC01_29165"
     CDS             complement(4077..5513)
                     /gene="dctP"
                     /locus_tag="VFC01_29165"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HZN83723.1"
                     /translation="MNAQTYMRLIAAVAGLATATTACSSNVPNRAGGSVDAQVSAVLT
                     LAVGNDGAPTQLTDWINQVHDLSHGSIRIEVKQAWRLGETDYEAQTVHDVEQAKVDMA
                     EVGARAMDRVGVTSFQALLAPMLVDSYNLQASVFQAGIPTQMLDSVPAAGVVGVAVLP
                     GPMRKVMGIAKPFLTPADFVGARIADQDSALTQMTLQTLGATAVPEPSGAKLHGVDGY
                     EQQLESIAGNRYDTIAKYTTANLNLWPRPLLIIMGKAAWAKLSGTQRDILRKAADTAL
                     QPAIDASRQEEQQALPVLCKNAMTLPTALPRQLAELRTALDPVYASLRRDASTNKWLD
                     QIQALKDQLHADPESVSCAGFGEASQKAAPIDGTYSRRSGVADIVAACHATPPPGTPA
                     HTTLEVVFDHGNLTQYQQTNGGTRDIGWRGTYQVFRDTLELTEAGTTAPMTLTWSLNG
                     STLTLSNLRNEYNCLDAAVWAHAWTKLK"
     gene            complement(5617..6276)
                     /locus_tag="VFC01_29170"
     CDS             complement(5617..6276)
                     /locus_tag="VFC01_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN83724.1"
                     /translation="MKIVIADDSLLVREGLARLLAEAGVQVVATAADAPQLLRELNRV
                     AADAAIVDIRMPPTFTTEGLDAAHAIRDAFPGMGIVVLSQFLESGYASQLLGQAPEHL
                     GYLLKDRVTDVAVILDALHRVIEGECVLDPTIVTTLIRRPRDPGPLSELTARERDVLA
                     LMAEGRSNTAIAQRLFVSAKTLETHVSRIFHKLHLTESSDDHRRVLAVLAFLQQPQRP
                     G"
     gene            complement(6254..>6799)
                     /locus_tag="VFC01_29175"
     CDS             complement(6254..>6799)
                     /locus_tag="VFC01_29175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZN83725.1"
                     /translation="RLRRVAALLERPAVKQDPALAEQLPRVLAEVADGQAELRQFAHG
                     VRPAVLGTAGLAAALPTLAAHTAHLTEVSVTVGRLSPAVEAALYFACAEALSNIAKHA
                     GASLAAVDVREEDGSVIATIIDNGRGGADPTGSGLRGLADRIEALGGTLLIAARPTGG
                     TMVEARIPPRGAVGREDCHRR"
BASE COUNT         1150 a         2065 c         2274 g         1310 t
ORIGIN      
        1 aactcaagaa accccaacgg agccttgctg tcccgctacc aggataaacc ggcgtgtcgc
       61 ccgcaatacc cgccacatcg tatagttctg tccacactgg acacatgagt tacgcgcaga
      121 agatatggca accggcttag acgagaaccg cggcagctga cgctgcaccg acgtgaagtt
      181 cacgaagccc ttcaccaagc gcgctcggtt caggaagacg aacaatggaa gcggcagtac
      241 ggactgcggt ccggcatcga agcgacgatc tcccaagcgg tccgcggatt tggtcttcgc
      301 cggtcgcgct accttgggct gacgaaaacc caactgcaac atgtgttaac tgcagcagcg
      361 atcaatctgg cccgggtgga cgcctggctc cgcgacatcc ctctcgccgg cacccgcagc
      421 catcacatcg cccgattgtt agctgctgca acatagttgc ggatttgcca acggtgtcaa
      481 gtttggtcga ataatccgca ggagcggttg aacaaggaga tccgtcgccg taccgacgtg
      541 gtcgggatct tccccaaccg cgccgccatc gtccgcctcg tcgacgcggt ccttgccgag
      601 cagaccgacg agtggaccga gcaacgcaga tacatgggtt tggaactgct tgccaaggcc
      661 cgcaaggaaa cctcgaagac caacaaccac ccgaaccggt gacgcagtcc caaaccgagc
      721 tgaccgccgc ctaaactacc gacaccggat caccgatggc cgtcactcgt acaccatccc
      781 cctcaatgtg acccactggc gccattcctc gggagtgtgg ggctcccgat cggcgtgcag
      841 aagcaggtcc gcgcacaggc cgcagcgacc gcgctgcagc gtgagcaggc gcaaagtgct
      901 gcggtcaagc ggcggcggtt ttctgccgcg ccattggttc cagtagtcgg tcagcgcgaa
      961 ttatcagggg atacccgcca gtgaccttgc ggtagcggac gatcggggtc caggccatca
     1021 acgggagata ggcgccactg gcgatgtcgc cgaacaccca atggttagat ctggacgggt
     1081 tgaaccggcc gaagtaccgg cggatgatcc accgcttcgg cttgccgggg tggctgtggc
     1141 agacccatcc gctgatgccg cccaggcgta cggcggctgc ccggcagtcg gggcatgcat
     1201 tggaggctgc agccgcctgt ggcgcggcgg gccggttcaa gctcggaatc ggttcgacag
     1261 tcagtttgac gagtcagtct cgtaggttgc ggagcggtcg gtgtttggcc atccgagcga
     1321 acccgggaat cctgccgtgg tgactagagg tggcgaattg gacgtgcgac cccgtcacgg
     1381 tgtcaaagat ggatccgatc tggaccccat aggtgtttta ggtcgtcggg catgccgcca
     1441 ccaactacaa gtcgtgttgt cagccggacg tcggtgcccg ggccgatcct tccactcgtc
     1501 ggatcctgct cgtagatcca cgaccagaat ccgccgaggc caccaccgag gttcccaccg
     1561 ataaggtcga cccccgactg gatgaaccga tattggtcgg gagacggaac caccccccgc
     1621 gtttccacct cgtaggcgtc gtcgctgccg ccggtagccc acgcgacgaa ctgggcgtag
     1681 gtccgggtcg gcacctcgcg gaccttcgcg atcgtgtgac cacgcaggcc gaccaggctg
     1741 actacatcga ttagatccgg cacctttctt cgcatctcga tcgggtcagg ctcggccagt
     1801 agtggcgaca gcgctgacgg gtcgaagaaa gtcggtgggt ccaggtgtcc gcgagacaac
     1861 tcaatcgcgt gccgggtcac cgcattgatc agttgtgtat agacgctgct ggccgcggtc
     1921 aacgttgccg ccggcgggag gtcctcgaaa aactccgggt tctgtcgtgc gaagtcgagg
     1981 tcgttccggg tctggatgta gtacagcttc ttcgtgcgag cgtcggcgag ggcagccacg
     2041 aacgcgtcct gctcctcagg cgttggcagc ggcagcggga tggtgtcgta cgtggcgacc
     2101 tcaatctcgt acgcgccagg gctgttcttt gcgatcgttg ggaactcctt atagcgcctg
     2161 agcacctcat cgatcgtaac ggtgggcgag atctccgcgc cggtgccggc cttttggtac
     2221 atcgtcgccg tgtattccga ccgcgtgctc tcgctctgat tgctcttgtt gaactcagcc
     2281 ttgaagctgc cgcctgccac cagaccattc gcctccgcct gcagggacgc ggcaagtttc
     2341 gactgagact ccgaggagac cgatgtaatc cggatcacgg cgtagaactc accgccagtc
     2401 tgcaacccgc gaacgaacga gtcgccgaac gccttcgtga actcctcaaa ccgcatggag
     2461 tccaacaggt cctgtgctgg tcctttggcc tggaaccccc ggccgcgctt gagcggattt
     2521 gccaccacga cgctcgcgac aacgaaggtt gagcttgagt tgtaagaaga ggattcggaa
     2581 aactgggctt tggccgaagc agagaagaaa ccgtaccgcc cttgagcctc gaaagacatt
     2641 ccgagggtgc tcattagccg ctcgtgcgag tttacgatct caatgtgagc tgatgctgtc
     2701 tggcccaagg tcgggggagg gacctcgaac ccttccagcg ctgtgccgac tgcaaggccg
     2761 gattgactgt tgaatcccat cgcgatctct tcgtcgttaa agatgatcct gcgcacttgt
     2821 gcgatcttct cggacatctc tacactcacc tttcagcaat tgaacgcggc taacccatca
     2881 gcttcgttga cgtcgtcgag catcgcgttc gaactcccag cacgtcgaat tcaaaaggcg
     2941 acaatcgaca acagcgggtc cgagagctct gcggtcgcat cgttcacgtc gcttgatatc
     3001 agctcagatg ttggcggcaa ctcaccgttc gaaatcaacg ggtgattact ggggtccagg
     3061 tcggcgaacg ggtctccgtc gacaagacat gtgacgcatg caaagaccgc atccaggccg
     3121 gcggagcccg cgacagtgat gtcggcgagg aaggcgggga cctcttcgaa tgagacgtcg
     3181 ctggtgtaga ggtctccgac gttggtgctc cacgcatcgt taacggacgt ggccaggagg
     3241 attagggcgg tgtgcgcgct gacaacgaga ttagcgttgg actgtattgc atcggcttga
     3301 gtgctggcgc tgtctgggtt tcctgcttct actgcaccgt cgtatcgctc caacgaggtg
     3361 accagggccg ccatggtgtc ggccacgatc agttggttgg ccgcgtacct gataaacgtt
     3421 gcttgtggtt cgtcggcttg tattgcttcc tcgagaagtt gcgcgtcgct gatggtgact
     3481 tggtcgaagt catcacgtgg agggtcgttt gccaagcgct ggtagcggtt gccgatggcc
     3541 caagcggcga agctgctgac gccgaggatc cccccgacaa gtagccccgc gggagcgccg
     3601 agcccggtcg cactgcccgc ggctgcaacg actattccag cacctccgag cacgacggcc
     3661 gtgtcgttgg cgacgacggc tgcgtttgcg gctgcggttt tggccttctg gcgttgctcc
     3721 gcactcacct cagtcatgtc gttgttccat tcgtttcact gcacccggct ggggtttcat
     3781 agacaatttg ctgccaaaag cgatctcggc aaatccgata acatggcggg aatcggtcgt
     3841 ggaagcgagt tatttgctct actgggcgtg ccgcgtcaaa tcgcgagccc ggtcgagatg
     3901 gcgagcccgg tcgagatggc gagcgtggat acggtcgcga tggtgacgag gcggatcatg
     3961 atgtgctcct ttcgcggagc gtgtttgggc aggggcaacc gagctaggta gggaggagcg
     4021 cgcgttcgac tgctgctatt agggctggac gcgcgaaccc cgatcgtgct gtgcggctac
     4081 ttgagtttgg tccaggcgtg tgcccagacg gctgcgtcaa ggcagttgta ttcgttgcgc
     4141 aggttgctca gggtcagggt tgagccgttg agggaccagg tgagcgtcat cggcgcggtc
     4201 gtgccggcct cggtgagttc gagagtgtcg cggaagacct ggtaggtgcc gcgccagccg
     4261 atgtctcttg tcccgccgtt ggtctgttgg tactgggtga ggttgccgtg gtcgaagacg
     4321 acctcgaggg tggtgtgtgc cggggtgccg ggcgggggcg tggcgtggca ggcggcgacg
     4381 atgtcggcca cgcccgaccg ccgcgagtag gtgccgtcga tgggtgccgc cttctgcgac
     4441 gcttcgccga acccggcgca ggatacggat tccgggtcgg cgtgcaactg gtccttcagt
     4501 gcctggatct ggtctagcca tttgttggtg ctggcgtcgc ggcgcagtga cgcgtagacg
     4561 gggtcaagtg cggtgcgcag ctcagctagt tgtcggggaa gggcggtcgg cagcgtcatg
     4621 gcgttcttgc agagcaccgg aagggcctgt tgctcctctt ggcgggaggc gtcgatcgcg
     4681 ggctgcaggg ccgtgtcggc tgccttgcgc aggatgtcgc gctgagtccc ggaaagcttc
     4741 gcccaggctg cttttcccat gatgatgagg agcggccgcg gccacaggtt gaggttggcg
     4801 gtggtgtact tggcgatcgt gtcgtaacgg ttgccggcga tcgattcgag ctgctgctcg
     4861 tatccgtcga cgccgtgcag cttcgcaccg gacggctccg ggaccgctgt ggctccgagg
     4921 gtttgcaggg tcatctgggt cagcgcggag tcctggtcgg cgatgcgggc gccgacgaag
     4981 tcggcggggg tcaggaaggg tttggcgatg cccattacct tgcgcatcgg ccctggcaag
     5041 accgcgacgc cgaccacccc ggcggcgggc acgctgtcga gcatttgtgt cgggatgccg
     5101 gcttggaaga cgctcgcctg caggttgtag ctgtccacga gcatcggggc gagcagcgcc
     5161 tggaatgagg tgacgcccac ccggtccatg gctcgtgcgc cgacctcggc catgtcgacc
     5221 tttgcctgct cgacgtcgtg gacggtctgc gcctcgtagt cggtttcgcc gagtcgccac
     5281 gcttgcttca cctcgatgcg gattgagccg tgggacaggt cgtgcacctg gttgatccag
     5341 tctgtcagct gcgtcggtgc gccgtcgttg ccgacggcta gggtgaggac cgcgctgact
     5401 tgggcgtcga cgctgccgcc ggcgcggttg ggcacgttgc tgctgcacgc cgtcgtcgca
     5461 gtcgcgagcc cggcgacggc tgcgatgagc cgcatgtagg tctgtgcgtt catggcgcac
     5521 ctccgtttcg acgccctcga cgctttcgac gctacggatt cgccgacggc cgtccatcag
     5581 ggacaggccg cagagttcgt cgccgttcca tcggggctag cccggacgct gcggctgctg
     5641 caggaacgcc aggacggcaa gcacccggcg gtggtcgtcg ctcgattcgg tgaggtggag
     5701 cttgtggaag atgcggctga cgtgcgtctc cagtgtcttg gcgctcacga acagccgctg
     5761 cgcgatcgcc gtgttcgatc tgccttcggc cattagagcc aggacatctc gctcgcgtgc
     5821 ggtcagctcg gacagcggtc cgggatcgcg gggccgccgg atgagtgtgg tgacgatggt
     5881 cgggtccagg acacattcgc cctcgattac ccggtgcagc gcgtcgagga tgacggcgac
     5941 gtcggtgacg cggtccttga gcaggtagcc gaggtgctcg ggtgcctggc cgagcagttg
     6001 tgacgcatag ccggactcga ggaactgcga gagcacgacg attcccatgc cgggaaaggc
     6061 atcccggatc gcgtgggcgg cgtcgagccc ttcggtggtg aacgtggggg gcatccggat
     6121 gtccacgatg gccgcatccg ccgccacgcg attcagctcg cgtaacagct gcggcgcgtc
     6181 cgcagcggtg gcgacgacct ggactccggc ctcggcgagc aggcgggcca gcccctcgcg
     6241 cacgagcagc gagtcatcgg cgatgacaat cttcacggcc cactgctccc ctcggcggga
     6301 tccttgcctc gaccattgtc cccccggttg gacgagcagc aatcaggagc gtgccgccga
     6361 gggcttcaat gcggtcggcc agcccgcgca gcccggagcc cgtcgggtcg gcgccgccgc
     6421 ggccattgtc gatgatcgtg gcgatcacgg acccgtcctc ttcgcggaca tcgaccgccg
     6481 ccaacgacgc acccgcatgc ttggcgatgt tgctcagcgc ctccgcgcat gcgaagtaca
     6541 gcgcagcctc gacggccggc gacagtctgc caaccgtcac cgagacttcg gtcaggtggg
     6601 cggtgtgcgc ggcgagcgtg ggcagcgctg cggcaagacc tgctgtccca aggacggcgg
     6661 gccgcactcc gtgggcaaat tgccgcagtt cggcctggcc gtccgcgacc tcggcgagca
     6721 ctcttggcaa ctgctcggcc agggcggggt cctgtttgac ggcgggtcgt tccagaagag
     6781 cggcgaccct tcgcaggcg
//
LOCUS       DASRJA010000648         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3467433,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000648 DASRJA010000000
VERSION     DASRJA010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3467433"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..1544
                     /locus_tag="VFC01_29180"
     CDS             351..1544
                     /locus_tag="VFC01_29180"
                     /inference="COORDINATES: protein motif:HMM:NF037564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HZN83726.1"
                     /translation="MKLPSRTASLAAEGDFHMAQISRRAVLLGVVGAGLAACSKPFAQ
                     PSVPPTAPPSPGPIPTLGPAPSIDARPRWPLTGKLLKNERLARHAAVAVKVPDNRREH
                     PQTGIDKADIVFVELEGYRDASGYSGTRLVPVFHSRMANSVAPVRSIRPVDIPLLSPI
                     HALIGNTGASRWVVNYVEHYRAHLEGLLSYMNALRTGSYGIDASRVYSLNGHNYYDKA
                     VICHPKVLARQTKRFRSGPPQPYFPFASTRDEVSAKEGKRGRSIRIPYQGDSYFMGYD
                     YDKKTKRYRRSMPWGPHVLTNGRRVSTDNVLVIKAKQHYGKVFRGGGHDEPLHDIIKT
                     RGKFYYFNRGRYVTGTWRKGKVQEPFEFTLNDGSPLKMAPGQTFVELPDIGAKVRIKA
                     RSVPM"
     gene            complement(1628..>2794)
                     /locus_tag="VFC01_29185"
     CDS             complement(1628..>2794)
                     /locus_tag="VFC01_29185"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZN83727.1"
                     /translation="LVAATLASGGLTAVQELRVRAGNPVRVMLAQRLSEAGEILARLG
                     GQCMAEYKYDGMRLQAHRTADGQIELFTRRQERVSTQFPDVVELLQTGLGPREAILEG
                     EVVAYDAAAGELRPFQEVMFRRRKHGIAEAARDVPVGLFCFELLYADGDDLTQLPYLD
                     RRARLADALTLSDRLRLTTAHEVATASALDAAFEQAVADGSEGLVCKSTGSTAIYQAG
                     ARGWLWIKLKRDYRTELADTVDLVVVGAYNGRGRRRGTYGAVLLAAYNPEEELYQTVG
                     RCGTGFSDAELAALPAKLAPLIRTERPARVDSRVPADVWFEPGIVMEVLSAELTLSPN
                     HTAGWGRLKDDAGLSMRFPRFTGRWRDDKEAQDATTTEQLVQLFQTARKVSTGA"
BASE COUNT          499 a          965 c          862 g          468 t
ORIGIN      
        1 gcgccaccgg cgaggtcgcc ccagtcgatc ggtccggcgg ggttgatcgc gaacacggtg
       61 gcggcgaagc ggtaggtgcg ggccagccgc ttcggcgtga cgccgatgag ctccttgaac
      121 cgcccacagc atcgccgacg ccccgccggc atcgcgtgcc gggatggtcg agccgcgctg
      181 ccgccagcat cgagccggcg ccgatcccgg acgagcgccc ggcatgtaat gcaactcccg
      241 tggctagttg tccgataagc gacattatgt caagttgacg gcgcgaccgg acaaccgagc
      301 gtcgtgtcgg ctccgacatt tcccggcggt cgatcccggt ggcccctacg atgaagctgc
      361 cttcccgcac cgcctcttta gcggccgagg gagactttca catggcgcag atcagccgcc
      421 gcgcggtcct gttgggcgtc gtcggtgctg ggcttgcggc ctgttcaaag ccctttgctc
      481 agccgagcgt ccccccgaca gcaccaccca gtcccggccc gatacccacc ctcggcccgg
      541 caccctcgat cgacgcgcgg ccacggtggc cgcttaccgg caagctgctg aagaacgagc
      601 gcttggcgcg gcacgccgcg gttgcggtga aggtgcccga caatcgccgc gagcatccgc
      661 agaccgggat cgacaaggct gacattgtct ttgttgaact cgagggctac cgggacgcgt
      721 cgggctacag cgggactcgg ctcgttccgg tcttccactc ccgcatggcg aacagcgtcg
      781 cgccagtgcg ctccattcgg ccggtagaca tcccgctact cagcccgatc cacgcgctga
      841 tcggcaatac cggcgcttcg cgttgggtgg tcaactacgt cgagcactat cgcgcccatc
      901 tcgaaggcct gctgtcgtac atgaacgccc tccgcaccgg ctcatacggc attgatgcat
      961 cgcgggtgta cagcctcaac ggtcacaact attacgacaa ggcagttatc tgccatccga
     1021 aggtgctcgc ccggcagacc aaacgcttcc gttcggggcc gccgcagccc tactttccct
     1081 ttgcctcgac ccgcgatgag gtcagcgcca aggaaggcaa acgcggccgc tcgatccgga
     1141 tcccctacca gggcgacagc tacttcatgg gctacgacta tgacaagaag accaagcgct
     1201 atcggcgcag catgccatgg ggtccacatg tgttgaccaa tggccgtcgg gtgtcgaccg
     1261 acaatgtgct cgtgatcaag gccaagcagc attacggcaa agtcttccgc ggtggcggcc
     1321 atgacgagcc gctgcacgac atcatcaaga cccggggcaa gttctactat ttcaaccgcg
     1381 gccgctatgt caccggcacc tggcgcaaag gaaaagtaca agagccgttc gaattcaccc
     1441 tcaacgatgg aagtccactc aagatggcac ccgggcagac ctttgtcgaa ctgcccgaca
     1501 tcggtgccaa ggtacgcatc aaggcccgat ccgtaccgat gtgatctctc agaggcccgg
     1561 ctctcggatg actcgatgac gccgtccagt gaaatgtcta acgcgatgtc cggaaagtca
     1621 tccgcgctca ggcgccggtg gacaccttgc gggcggtttg aaacagctgc accaactgct
     1681 cggtcgtcgt agcgtcctgc gcctctttgt cgtcccgcca ccggcccgtg aagcgaggga
     1741 agcgcatcga cagaccggcg tcatccttca gccggcccca gccggcggtg tggttgggcg
     1801 acaacgtgag ctcagcgctg agtacctcca tcacgatgcc cggttcgaac cacacgtcgg
     1861 ccggaacgcg ggaatcgacg cgtgcgggcc gctctgtgcg aatcaacggc gccagcttcg
     1921 ccggcaacgc ggccagctca gcgtcggaaa atccggtgcc gcatcggccg accgtctggt
     1981 agagctcctc ctccgggttg tatgcggcca gcaggaccgc gccgtaggtg cctcgccgcc
     2041 ggcctctgcc gttgtacgcg ccgacaacaa ctaggtcgac tgtgtcggcg agttcggtac
     2101 ggtaatcccg cttgagcttg atccacagcc agccgcgggc gcccgcctga tagatcgccg
     2161 tcgagccggt cgacttacac accagaccct cggagccatc ggcgactgcc tgctcgaacg
     2221 cagcatcaag cgccgacgct gtggctacct cgtgcgctgt ggtcagccgc agtcgatccg
     2281 acaaggtgag tgcgtcggca agccgcgccc gacggtccag atagggcagc tgggtgagat
     2341 cgtccccatc ggcgtacagc agctcgaagc agaacagccc gacgggcaca tctcgcgcgg
     2401 cttcggcgat gccatgcttc cggcgacgga acatgacctc ctggaagggc cgcagctcgc
     2461 cggccgcggc gtcgtatgcg acgacctcgc cttcgaggat cgcctcgcgc gggccgaggc
     2521 cggtttgcag cagctcaacc acgtcgggga actgggtgct cacccgctcc tgccgccgag
     2581 tgaacagctc aatctggccg tcggccgtgc gatgggcctg cagtcgcatc ccgtcgtact
     2641 tgtattcggc catgcactgc ccgcccaacc tggcgaggat ctcgccggcc tcagacaacc
     2701 gttgggccag catcactcgc accgggttgc cagcccgtac ccgcaactcc tggacagcgg
     2761 ttagtccgcc ggatgccagc gtggccgcga cgag
//
LOCUS       DASRJA010000649         7459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_646363,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000649 DASRJA010000000
VERSION     DASRJA010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646363"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1711)
                     /locus_tag="VFC01_29190"
     CDS             complement(71..1711)
                     /locus_tag="VFC01_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HZN83728.1"
                     /translation="MTEPDREIVSPTTLAPDRRSIDNRDLPSRTDTIGAALSGVIGGP
                     VGRHALIGRQRLLTPLRVMLIIALVFLALGYSTKAACLQTTGAGTADQRVGNWQNQRA
                     YYELCYSDTVPLYTAELLNLGKFPYKSSWIETDSDGKPRKQYDGNIAVRYMEYPVLTG
                     IYQYLSMALAKTYTALSKLVSVPIIAEVVMFFNIAAFGLALAWLATVWASARLAGSRR
                     IWDAALVAGSPILIFQMFTNFDALATAFATGALLAWARRRPWLAGALIGLGVAAKLYP
                     LLLFVPLVLLGIRTGRLRQVGKTAIATVLTWLAVNLPIMVLFPRGWSEFFRLNTRRGD
                     DMDSLYNVVKSFTGWGGFDQDLGFWQPPTVLNVVTAVLFVSCCIAITYVVLTAKQRPR
                     LMQVAFLVVAAFLLTNKVWSPQFSLWLVPLAVLALPHRRILLAWMTIDALVWVPRMMF
                     LYGEQNRGLPEQWFTATVLLRDVAVMALCALVIRQIYRPELDLVRCRGQVDDPSGGVF
                     ERAPDDPPRWLPDWLRPEAGRTRVTPAPEPEPELAEVK"
     gene            complement(1708..4008)
                     /locus_tag="VFC01_29195"
     CDS             complement(1708..4008)
                     /locus_tag="VFC01_29195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZN83729.1"
                     /translation="MRGGPPSQLRDPIDVVKRALDGTPPPAPPRKPPPPPPRRPPGGG
                     GPRGGPPPAPKASFREQINWKWVRRALYVTMAVLIVLPIVTFGMAYLIVDVPKPGDIR
                     TNQVSTILASDGTELAKIVPPEGNRVDVNIDQIPVHVRDAVMAAEDRDFYSNPGFSFT
                     GFARAFKNNIFGGDLQGGSTITQQYVKNALVGDARSGIGGVIRKAKELVISTKMSSEW
                     SKDQVLQSYLNIIYFGRGAYGISAAAKAYFDKPVEQLTVGEGALLAALIQRPSGLDPA
                     VNPEGAAERWNWVLDGMVDIGALSKEDRAAQQFPPTVPPEQARSQNQTTGPNGLIERQ
                     VQQELLDLFNIDERSLNTQGLQITTTIDPKAQKAAEEAAAKYMDGQDPDMRTAIVSID
                     PKTGAVKAYYGGTDAQGFDFAQAGLPTGSSFKVFALVAALQQGMGLGYQVDSSPVEVN
                     GIKVTNVEGAGCGTCNIAEALKRSLNTSYYRLMLKLKNGPADVADAAHKAGIAESFPG
                     VDHTLSEDGKGGPPNNGVVLGQYQTRVLDMASAYATLGASGVYHKPHFVQKVVNSRGE
                     VLFDASQEDNTGEQRIDKAVADNVTSAMQPIAAYSNGHNLAGGRPSAAKTGTNQLGDT
                     GANRDAWMVGFTPSLSTAVWVGTTEGTKPLENKWGSPVYGSGIPSDIWKSTMDGALED
                     TENESFPKPAEIGGYAGVPQAPAPPPPSAPPTPSESVIQPTIEVAPGITIPIGPPTTV
                     PVVPPVPVGPAPGPVGVPPAGSPPPP"
     gene            complement(4026..4160)
                     /locus_tag="VFC01_29200"
     CDS             complement(4026..4160)
                     /locus_tag="VFC01_29200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83730.1"
                     /translation="MSALDARLMASGRVNPTRPEAFAHNLARTGRRDIGPQALPPTTG
                     "
     gene            complement(4190..4615)
                     /locus_tag="VFC01_29205"
     CDS             complement(4190..4615)
                     /locus_tag="VFC01_29205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 domain-containing protein"
                     /protein_id="HZN83731.1"
                     /translation="MRLQRQVVDYALRRRSLLAEVYSGRTGVTEVCDANPYLLRAAKF
                     HGKPSSVMCPICRKEQLTLVSWVFGDHLGAVSGSARTAEELVLLASRYDEFSVHVVEV
                     CRTCSWNHLVKSYVLGAVPPPKKSRSTRTARGRARTASE"
     gene            4728..5588
                     /locus_tag="VFC01_29210"
     CDS             4728..5588
                     /locus_tag="VFC01_29210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HZN83732.1"
                     /translation="MATRHTPGTRAKDSDRNDTCQILDSALADGQLSMDEHRTRVALA
                     TGAETLGDLQSLVSDLQTNNAPVQLPDLKKRPKVPGAASGWGMRLAVTGVLVLLGVAI
                     GWGLYGNTPSPLSFTSDPGAKSDGIGPVVLTPPTQLQSLGGLTGLMEQTRKRFGDTMG
                     YRMIIYPEYAVLDRADPNDNRRKVSYTYRGGWGDSTSTSAVSDDDVLVDLGKFDPKAI
                     VGVFRGAPQTLHIKQSDVTSSYINIDPAKDPTNPDPLTVTVYVSSDFGSGYIEMSGDG
                     SVKQINQPNS"
     gene            5702..6247
                     /locus_tag="VFC01_29215"
     CDS             5702..6247
                     /locus_tag="VFC01_29215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZN83733.1"
                     /translation="MLELAILGLLLESPMHGYELRKRLTGLLGAFRAFSYGSLYPALR
                     RMQADGLIVEDAAPEGIPKMRRARRVYQLTDAGKQRFAELVADTGPQNFSDDGFGVHL
                     AFFNRTPAEARMRILEGRRRQVEERREGLREAVARASSSIDRYTRQLHQLGLESSERE
                     VKWLNELIAAERTAQGRAEQP"
     gene            6251..7360
                     /locus_tag="VFC01_29220"
     CDS             6251..7360
                     /locus_tag="VFC01_29220"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HZN83734.1"
                     /translation="MTAVSERENVVMTENTDIRVAIVGVGNCASSLVQGVQYYQDADE
                     TATVPGLMHVRFGPYHVRDVKFVAAFDVDAKKVGFDLSEAIFASENNTIKIADVPPTN
                     VTVQRGPTLDGIGKYYADTIEVSDVDPVDVVKVLKDSKVDVLVSYLPVGSEEADKFYA
                     QCCIDAGVAFVNALPVFIASDPVWAKKFEDAGVPIVGDDIKSQVGATITHRVMAKLFE
                     DRGVQLDRTMQLNVGGNMDFLNMLERERLESKKISKTQAVTSNLQREFKTKDVHIGPS
                     DHVGWLDDRKWAYVRLEGRAFGDVPLNLEYKLEVWDSPNSAGVIIDAVRAAKIAKDRG
                     IGGPVVAASAYLMKSPPKQLPDDIARAQLEDYIKG"
BASE COUNT         1299 a         2504 c         2405 g         1251 t
ORIGIN      
        1 gagttcgagg accgaggagc gcacgaattg aagggcgtgc ccggcgagtg gcacctgttc
       61 gccgttgcgt ctacttaact tccgcgagtt cgggttcggg ctcgggcgcg ggcgtcacac
      121 gcgttcggcc cgcctccggg cgcaaccagt ccggcaacca gcgcggtggg tcatcgggcg
      181 cgcgctcgaa cactccgccc gacgggtcgt cgacctgccc gcggcaccgc accaggtcca
      241 gctcgggtcg atagatctgg cgaataacca acgcgcacaa cgccatcacc gcgacgtcac
      301 gcagcagcac cgtcgcggtg aaccactgct cgggcaggcc gcggttctgc tcgccgtaca
      361 ggaacatcat ccgcggcacc cacaccagcg catcgatcgt catccacgcc aacagaatcc
      421 ggcgatgcgg cagcgccagc accgccagcg gcaccagcca cagcgagaac tgcggactcc
      481 acaccttgtt cgtcaacagg aacgcggcca ccacaagaaa cgccacctgc atcagccgcg
      541 gccgctgctt cgccgtcagc acaacgtatg tgattgcgat acaacaggat acgaacagga
      601 cggcggtcac cacgttgagc accgtcggcg gctgccagaa cccgaggtcc tggtcgaagc
      661 cgccccagcc ggtaaacgac ttcaccacgt tgtacagcga gtccatgtcg tcgccgcggc
      721 gggtgttgag ccggaagaat tccgaccacc cccgcggaaa cagcaccatg atcggcaggt
      781 tcaccgccaa ccacgtcagt accgtggcga tcgccgtctt gcccacctga cgcagtcgtc
      841 ccgtccggat gccgagaagc accaacggca cgaacagcag cagcgggtac agcttggccg
      901 ccacgccaag cccgatcagc gcgcccgcca gccagggtcg gcgccgcgcc caggccagca
      961 gtgcacccgt cgcgaaagcc gttgccagcg cgtcgaaatt cgtgaacatc tgaaagatga
     1021 ggatcggcga tccggccacc agcgccgcgt cccagatgcg tcgtgacccc gccagtcgcg
     1081 cgctggccca caccgtcgcc aaccacgcca gcgccaaccc gaatgcggcg atgttgaaga
     1141 acatcaccac ctcggcgatg atcggcaccg acaccagctt gctcagcgcg gtgtatgtct
     1201 tggccagcgc catcgacagg tactggtaga tcccggtcaa taccggatat tccatgtacc
     1261 gcacggcgat gttgccgtcg tactgtttgc gcggcttgcc gtcgctgtcc gtctcgatcc
     1321 aactggactt gtacggaaac ttgccgaggt tcaacaactc tgcggtgtac agcggaacgg
     1381 tgtcggaata gcacagctcg tagtaggccc gctggttctg ccagttgccc actcgctgat
     1441 cggcagtgcc cgcgccggtg gtctgcagac acgccgcctt tgtcgagtag cccaacgcga
     1501 ggaacacgag cgcgatgatc agcatcaccc gcagcggcgt cagaaggcgc tggcggccga
     1561 tcagggcatg ccggcccacc gggccgccga tcacacccga aagcgcggca ccgatcgtgt
     1621 cggtgcgact gggcaggtca cgattgtcaa tgctgcgccg atcgggcgct aacgtcgtcg
     1681 gcgagacaat ctcgcggtcc ggttcggtca cgggggcgga ggagatccag ccggcggcac
     1741 tcccaccggc cccggcgcgg gtcccaccgg caccggcgga acgacaggca cggtcgtcgg
     1801 cgggccgatc gggatcgtaa tgccaggcgc gacttcgatc gtcggctgaa tgacgctctc
     1861 cgacggcgtt ggcggcgcac tagggggcgg cggcgccggt gcctgcggca cgcccgcata
     1921 gccgccgatc tcggccggct tcgggaacga ctcgttctcg gtgtcctcca gcgcaccgtc
     1981 catcgtcgac ttccagatgt ccgacggtat tcccgacccg taaaccggtg agccccactt
     2041 gttctcgagc ggcttggtgc cctcggtggt gcccacccag accgccgtgg acaacgacgg
     2101 ggtgaatccg accatccacg cgtcacggtt ggcgccggtg tcaccgagct ggttggttcc
     2161 cgtcttggcc gccgacggac gcccgcccgc caggttgtgc ccgttggagt acgcggcgat
     2221 cggctgcatc gcggacgtga cgttgtcggc cacggccttg tcgatgcgct gctcgccggt
     2281 gttgtcctcc tggctggcgt cgaacaacac ctcgcccctc gagttgacga ccttctgcac
     2341 gaagtgcggc ttgtgataca cgcccgacgc tcccagcgtg gcatatgccg acgccatgtc
     2401 gagtacgcga gtctggtact ggcccagcac aactccgttg ttcggcggtc cgcccttgcc
     2461 gtcctcggac agcgtgtgat ccacacccgg gaagctttct gcgatcccgg ccttgtgcgc
     2521 ggcgtcggcg acgtccgccg gcccgttctt gagcttgagc atcagtcggt agtagctggt
     2581 gttcagcgac cgcttcagcg cctcggcgat gttgcacgtg ccgcacccgg cgccctcgac
     2641 gttggtgacc ttgatgccgt tgacctcgac cggcgagctg tccacctgat agcccagacc
     2701 catcccctgc tgcagcgcgg ccaccaacgc gaacaccttg aacgacgatc ccgtcggcaa
     2761 cccggcctgc gcgaagtcaa acccctgcgc gtcggtgccg ccgtaatacg ccttcaccgc
     2821 accggttttc gggtcgatcg aaacgatcgc ggtgcgcatg tccgggtcct gaccgtccat
     2881 gtacttcgcc gccgcctcct ccgcggcctt ctgcgccttc gggtcgatcg tcgtggtgat
     2941 ctgcagaccc tgagtgttca gcgaccgctc gtcgatgttg aacaggtcga gcaactcctg
     3001 ttggacctgc cgctcgatca gaccgttcgg gccggtggtc tggttttgcg agcgagcctg
     3061 ctccggcgga accgtcggcg ggaactgttg cgcggcgcgg tcctcctttg acagcgcgcc
     3121 gatgtcgacc atgccgtcga gcacccagtt ccaccgctcc gcggcgccct cggggttgac
     3181 ggcgggatcc agccccgacg gccgctggat gagcgcggcg agcagcgcac cctccccgac
     3241 ggtcagctgc tcgacaggtt tgtcgaagta agccttggcc gccgctgaga tgccgtacgc
     3301 gccgcggccg aaatagatga tgttcagata ggactgcagc acttggtctt tggaccactc
     3361 gctggacatc ttcgtcgaga tgaccagttc cttggccttg cggatcacac caccgatacc
     3421 cgagcgcgcg tcaccgacca gtgcgttttt gacgtactgc tgggtgatcg tcgaaccgcc
     3481 ctgcaggtcg cccccgaaga tgttgttctt gaacgcacgc gcgaagccgg tgaacgagaa
     3541 gccgggattg gagtagaagt cgcgatcctc ggcggccatc accgcgtcac gcacgtgcac
     3601 cggaatctgg tcgatgttga cgtcgacgcg attgccctcg ggcggaacga ttttcgccaa
     3661 ctcggtgccg tcgctggcca ggatcgtgga cacctgattg gtccggatgt cgccgggctt
     3721 gggcacgtcg acaatgagat aggccatccc gaacgtgacg atcggcagca cgatcagcac
     3781 ggccatcgtg acgtagagcg cgcggcgcac ccacttccag ttgatctgct cgcggaagct
     3841 ggccttcggc gcggggggtg gccctccgcg tggtccaccg ccgccgggtg gtcgccgtgg
     3901 cggcgggggc ggtggtttcc tcggtggcgc cggcggcggt gtcccatcca gcgccctctt
     3961 gacgacgtcg atcgggtcac gcagctgcga cggcgggccg ccacgcaccg gcggcagtat
     4021 cgcggtcagc cggtcgtcgg ggggagcgcc tgcggcccga tgtcgcggcg gcccgtcctg
     4081 gcgaggttgt gggcgaacgc ctccgggcgg gttgggttga cgcgccccga cgccatcagc
     4141 cgcgcgtcca gagcggacat cactggctga ccggtcgtgg cgcccttcgc tattcactgg
     4201 ccgtgcgcgc acgcccgcgc gccgtccgag tgctgcgcga cttcttcggg ggtgggaccg
     4261 cgccgagcac atacgacttg accaagtgat tccagctgca ggtgcggcat acctccacca
     4321 cgtgtacgga aaactcgtca tatcgggacg ccaacaggac caactcctcg gcggtgcgcg
     4381 ccgatccaga cacggcgcct aggtggtcac cgaataccca cgacaccaag gtgagctgtt
     4441 ccttgcgaca gatcggacac atcaccgagc tgggcttccc atggaacttc gcggcccgca
     4501 gcaggtaggg gttggcgtca cagacctccg tgacgccggt acggcccgag tacacctcgg
     4561 ccagcaggga ccggcgccga agcgcatagt ccaccacctg tcgctgcagt cgcacggtga
     4621 ccagagtacg tcggccgtgc ggccgccgag gcatgactgc gccgccacac tccggggttg
     4681 gctatctggt cagcggcgat ggccaccgaa ctcttacgat catcgtcgtg gcgacgcggc
     4741 atacaccggg aacgcgggcg aaggacagcg accgcaatga cacctgccag atcctcgaca
     4801 gtgcgctggc ggatgggcag ctgtcgatgg atgagcaccg gacgcgggtg gcgctggcga
     4861 ccggagccga gacgctgggc gatctgcagt cattggtgtc ggacttgcag accaacaacg
     4921 ctccggttca actgcccgac ctcaagaagc ggcccaaggt gccgggcgcg gccagcggat
     4981 ggggcatgcg ccttgcggtg accggcgttc tcgtcctcct cggcgtcgcg atcggctggg
     5041 ggctgtacgg caatacgccg tcgccgctga gcttcacctc tgaccctggt gccaagtccg
     5101 acgggattgg gccggtggtg ctcactccac ccacgcagct ccagtcgctg ggcggcctca
     5161 ccggattgat ggagcagacc cgcaagcgtt tcggcgacac catgggctac cggatgatca
     5221 tctatcccga gtatgcggtg ctggatcgtg cggatccgaa cgacaaccgt cggaaggtga
     5281 gctacaccta ccgcggtggc tggggcgatt cgacgagcac ctctgctgtc tccgatgacg
     5341 acgtgctcgt cgacctcggc aagttcgacc cgaaggcgat tgtcggtgtg ttccgtggcg
     5401 caccccaaac cctccacatc aagcagtccg atgtcacaag tagctacatc aacatcgatc
     5461 cggcgaagga tcccaccaac cccgacccgc tgactgtgac cgtctacgtc tccagcgatt
     5521 tcggcagtgg ctacatcgag atgtccggcg acggaagcgt caaacagatc aaccaaccca
     5581 acagctgacg tccacagctg gtcagcggtg cgcaaccttt tgttggcgcc gaatatatcg
     5641 gcgcgatact atgggccgag gtgtcgacac gtcgtagcgg ccaagcaaag gaggtggttc
     5701 gatgctggag ctcgccatcc tgggtcttct gctcgaatcg ccgatgcacg gctacgagct
     5761 gcgcaaacgg ctgaccggcc tgctcggcgc cttccgggcg ttctcgtacg gctcgctcta
     5821 cccggcgttg cgacgcatgc aggccgacgg cctgatcgtc gaggacgccg cgcctgaggg
     5881 aatccccaag atgcgccgcg cgcggcgcgt ctaccagttg accgacgccg gtaagcagcg
     5941 cttcgccgaa ctcgtcgccg ataccggccc gcagaacttc tccgatgacg gattcggcgt
     6001 tcacctcgcg tttttcaacc gcactccggc cgaggccagg atgcggatcc tcgaggggcg
     6061 ccgccgtcag gtggaggagc gtcgtgaggg cctgcgtgaa gcagtggcgc gggccagcag
     6121 ctcgattgac cgctacaccc ggcagctgca ccagctcggg ttggagtcca gcgagcgcga
     6181 agtcaagtgg ctgaacgaat tgatcgcggc cgaacgcacg gcgcaggggc gcgccgagca
     6241 accgtgagct atgaccgcag taagtgagag ggagaacgtc gtcatgactg agaacaccga
     6301 catccgggtc gccatcgtcg gcgtgggcaa ctgcgcgtcc tcgctggtgc agggcgtgca
     6361 gtattaccag gacgccgacg agacggccac ggtgcccggt ctgatgcacg tgcgcttcgg
     6421 cccgtatcac gtccgggacg tcaagttcgt cgccgccttc gacgtcgacg ccaagaaggt
     6481 cggcttcgac ctctcggagg cgatcttcgc gtcggaaaac aacaccatca agatcgccga
     6541 cgtgccgcct accaacgtca cggtgcagcg cggcccgact ctcgacggca tcggcaagta
     6601 ctacgccgac accatcgagg tgtctgacgt cgatccggtc gacgtggtca aggtgctcaa
     6661 ggacagcaag gtcgacgtgc tggtgtccta cctgccggtt ggctccgagg aggccgacaa
     6721 gttctacgcg cagtgctgca tcgacgccgg cgtggcgttc gtcaacgcac tgccggtgtt
     6781 catcgcgtcg gatccggtgt gggccaagaa gtttgaggat gccggcgtgc cgatcgtcgg
     6841 cgacgacatc aagagccagg tcggtgcgac catcacccac cgtgtgatgg ccaagctgtt
     6901 cgaggaccgc ggagtgcagc tggaccgcac gatgcagctc aacgtcggcg gcaacatgga
     6961 cttcctcaac atgctcgagc gcgagcggct ggagtccaag aagatctcca agacccaggc
     7021 cgtcacctcc aacctgcagc gcgagttcaa gaccaaggac gtccacatcg gtccgtccga
     7081 tcacgtcggc tggctcgacg accgtaagtg ggcctacgtc cggctcgagg gtcgcgcctt
     7141 cggtgacgtg ccgctgaacc tggagtacaa gctcgaggtg tgggactcgc cgaactcggc
     7201 gggcgtcatc atcgacgcgg tccgtgccgc gaagatcgcc aaggaccgcg gcatcggcgg
     7261 cccggtggtg gccgcgtcgg cgtacctgat gaagagcccg ccgaagcagc tgcccgacga
     7321 catcgcccgc gcgcagctcg aggattacat caagggctga tacggcaatg ccggcaaaat
     7381 gcggggaccc tatcaagggt ccccgttttg catgttgctc ttgtcggggc ggctcgaggt
     7441 ttacgcagtt gaccggggg
//
LOCUS       DASRJA010000650         5065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3509246,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000650 DASRJA010000000
VERSION     DASRJA010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3509246"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFC01_29225"
     CDS             <1..251
                     /locus_tag="VFC01_29225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83735.1"
                     /translation="YLLNAVAMLVTTEPGREHGTDPDARDDAIRRKTASLSGLPVRRY
                     PNIVSSASVLAACPSPDAYFSLGLDMLIAGVTAITDEG"
     gene            383..1648
                     /locus_tag="VFC01_29230"
     CDS             383..1648
                     /locus_tag="VFC01_29230"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HZN83736.1"
                     /translation="MNENRTALRTALSEPLTLRSGITLPNRLAKAATSEHLADRRGAP
                     TNQLITAYRALARSGAGLLISGNVMVDGAALEAPRNVVIEDGRHREQLRRWAAVTDGT
                     DAKFILQLSHPGRQTMRGNRLPGRRHDVVGPSEVPLAVGGNRLFQAPRALSDAEIKAI
                     IARFARAAEIAADAGFHGVQIHAAHGYLFNQFLSPLVNQRTDRWGGSLENRMRLLVET
                     VREIRATTSARFLIAVKLNSADFQRGGFDSDDSLTVARALDKEGIDLLEISGGTYEST
                     AMVTGMPQRESTRAREAYFLEFAERFSTEVSVPIMLTGGFRTRQGMNDALDSGAIDVV
                     GLARPITYEPDLPQRLLDGTAEKSLVTPKTLGHKNIDDLLNSAWHQQQIARMGRGRAV
                     RPRRGPAVALVIALLTTARDLLLPKLAPR"
     gene            1720..2502
                     /locus_tag="VFC01_29235"
     CDS             1720..2502
                     /locus_tag="VFC01_29235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009885401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN83737.1"
                     /translation="MDLQLTGKVVVVTGAASGIGQASARVLTHEGAVVVGVDRDPIDT
                     EPGAPATAVQADLTDPATPDRVIATVLEQHGRIDALVNNAGGLHARTSFLEITDEQWL
                     ATFNLNFHAARRMSRAAVPAMVASGGGSLVHLGSDSARLPEVGNLDYAAAKLPLLALS
                     KSLATEFGPQGIRSNVVVPGPTRTPLYDRPGGFGDQAATLWGTDKETAITRMVTEIRP
                     LLTGRMGQPDDIAQVVAYLVSPLSRQVTAAEWSVDGGAQRQV"
     gene            2528..2962
                     /locus_tag="VFC01_29240"
     CDS             2528..2962
                     /locus_tag="VFC01_29240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005257986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83738.1"
                     /translation="MNSPIAEPNSPRTSPLAGTVIAENFAGWSAEIRQEFVDNAHNRQ
                     VGSVLLSETDQVKVWSIRLAPGERVPAHRHVLDYFWTALTDGISLQHNDDGTTRRVAY
                     RAGETRHFSFPGDEYILHDLYNDGPSELAFLTVEHKSNAKQD"
     gene            2986..4011
                     /locus_tag="VFC01_29245"
     CDS             2986..4011
                     /locus_tag="VFC01_29245"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZN83739.1"
                     /translation="MKVLVTGATGYLGAVAAEALAARGHQVLGLARSERSASALRERG
                     LEPVMGDFGDPVSLANAVHDARPDVVVSTASVGGASGDQAAFARDGEAVRAMREALAD
                     HGGALVFTSGSAVFGVFNGGDATDTVYDEDTRLPLPASVFAPDSAGVHPLLAAGFGAA
                     MAARVETERAVLADGDVRGVVVRPGLVYGRGGNSDLRSLIDRARKEGHAGHWGSGGTT
                     QSYVHVDDLAELFGLAVELAPHGAILHGVAGDVTQRDLARAINRMIGTDEHTDSLSLL
                     QMLGIDADTVGNIGASFTPPPSAASGISLSLNKRLSSDKTRKLLDWSPKRTDVGDDIA
                     FGSYANA"
     gene            4064..5020
                     /locus_tag="VFC01_29250"
     CDS             4064..5020
                     /locus_tag="VFC01_29250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN83740.1"
                     /translation="MTDINRTTVRIPTPSGFDIEAWFYLPEGPGPFPAVVMGHGLGAV
                     KVGGLASFAERFCREGFAAVVIDYRQWGGSEGQQPRDELNVPRQRDDYSAAIGWAAAQ
                     PNIDEHRIFVWGTSFAGMHATALAASDHRLAGAIAQCPLVDGLAGSRLVRPTRSLALF
                     ATAIIDRLGSFLGRATIYLPVNVAPGEWGIADTSDALFGKQVMHPREPIDWHNRMAAR
                     SLLSIPAHRPVRHARDINIPILLIVAEHDTQAPVGPALAMADRAPRAELHRSTGGHYD
                     VYDGGAAFGDVIEWEVDFLRRHARIEPTAPQTPSDSSSTPKP"
BASE COUNT          921 a         1825 c         1542 g          777 t
ORIGIN      
        1 gatatctgct gaacgcggtg gccatgcttg tgaccaccga gccggggcga gagcatggca
       61 cggacccaga cgcccgtgac gatgcaatcc gccgcaagac cgcgtcgttg agcgggctgc
      121 ctgtgcggcg ttatcccaac atcgtgtcat ccgccagcgt gctggccgcg tgcccgagtc
      181 cagatgccta cttcagcctc ggacttgaca tgctgatcgc cggcgtcacc gccatcacgg
      241 acgaaggtta gcccgaggca cacccggcct gacctagtag gacgggcgcc tctcatcccc
      301 gccgtttgac aactgtacta tgtacatgct tacctgtacg atgtacatgt acgacataca
      361 acgacatgaa cggcggactg agatgaatga gaaccgaacg gcactccgga cggcgctgtc
      421 cgagccactt accctgcgca gcggaatcac cctgccgaac cggcttgcga aggcggcgac
      481 cagcgagcat ctggccgacc gtcgcggggc accgaccaac cagctcatca ccgcctaccg
      541 ggcgctggcg cgctctggcg ccgggttgtt gatcagcggc aacgtcatgg tcgacggcgc
      601 ggctctggaa gcaccgcgca acgtggtgat cgaggacggc cgacaccgcg agcagttgcg
      661 tcggtgggcc gcggtgaccg acggcaccga tgcgaagttc atcctgcagc tgtcccaccc
      721 agggcggcag accatgcgcg gcaaccggct gcccgggcgt cgtcacgacg tcgtcggccc
      781 gtcagaggtg cccctcgctg tgggtggcaa ccggctcttc caggcgccgc gcgctctgtc
      841 tgacgcggag atcaaagcga tcatcgcccg cttcgctcgc gccgctgaga tcgccgccga
      901 tgccggcttc catggcgtgc agatccacgc cgcgcacggc tacctgttca accagttcct
      961 ctcgccgcta gtcaaccagc gcaccgaccg ttggggcggc tcgctggaga accgtatgcg
     1021 actgctcgtc gagacggtcc gagagatccg ggcgacgact tctgctcggt tcctcatcgc
     1081 cgtcaaactg aattccgccg atttccagcg cggcggcttc gactcagacg actccctcac
     1141 cgtcgcccgt gccctcgaca aggaaggcat cgacctgctg gagatctcgg gcggcacata
     1201 tgagagcacc gccatggtca cgggcatgcc acagcgagaa tcgacgcggg cgcgggaggc
     1261 ctacttcctc gaattcgcgg aacggttctc cacggaagtg tcggttccga tcatgctgac
     1321 gggcggattc cgcacccggc agggcatgaa cgacgcattg gacagcggtg ccattgacgt
     1381 cgtcggcctc gcccgcccga ttacttacga gccggatctg ccccaacgcc tcctggatgg
     1441 aactgccgag aagagcctgg tgacgccgaa gaccctgggc cacaagaaca tcgacgacct
     1501 cctcaacagc gcttggcacc agcagcagat cgcccggatg ggccgtggca gggccgtgcg
     1561 gccccgacgc ggtcccgcgg tagccctcgt aatcgccctg ctcaccactg cccgggacct
     1621 cctacttccc aagttggcgc cccgctgacc tcagatttcc cccgacccgg cgctgcggcg
     1681 acctgccctt accgctgaac ccatccccga aggagaacca tggacctaca actcaccggc
     1741 aaggtcgtgg tcgtcaccgg cgccgcctca ggcatcggac aagccagcgc ccgcgtgctc
     1801 acccacgaag gagccgtcgt cgtcggtgtc gaccgcgatc cgatcgacac cgaacccggc
     1861 gcccccgcca ccgcggtcca agcggatctc accgaccccg ccacaccaga ccgcgtgatc
     1921 gccaccgtgc tcgaacagca tggccggatc gatgctctcg tcaacaacgc cggcggactt
     1981 catgcccgca ccagcttcct cgagatcacc gacgagcaat ggctggccac tttcaacctc
     2041 aacttccacg ccgcccgccg catgagccgc gccgccgtgc ccgccatggt cgcgagcggt
     2101 ggcggcagcc tcgtccacct cggcagcgac tccgcgcggc tgccggaagt cggcaacctt
     2161 gactacgcgg cggccaaact tccgctgttg gcgctgtcca aatccctggc caccgagttc
     2221 ggtccccaag gcatccggtc gaacgtcgtg gtccccggac ccacccgcac accgctctac
     2281 gaccggccgg gcggcttcgg cgaccaggcc gccacgctgt ggggcacaga caaggaaacc
     2341 gcgatcaccc gcatggtgac cgaaatccga ccactgctga ctggccggat gggccagccc
     2401 gacgacatcg cccaggtcgt cgcgtacctc gtctcaccgc tctcgcgcca ggtcaccgcc
     2461 gccgaatgga gcgtcgacgg cggcgcgcag cgccaagtct gaccccacca tcaacaagga
     2521 gaatccgatg aattcgccca tcgcagaacc gaactcgccg cgtacatcac cacttgccgg
     2581 aacggtcatc gccgagaact tcgccggctg gagcgccgaa atccggcagg agttcgtcga
     2641 caacgcccac aaccgccagg tcggatcggt gctgctctcg gagaccgacc aggtcaaggt
     2701 gtggagcatc cggctcgcgc ccggtgaacg ggtcccggcg caccggcacg tgctggacta
     2761 cttctggacc gccctcaccg acggaattag cctgcagcac aacgacgacg gcaccacccg
     2821 ccgcgtcgcc taccgagccg gcgaaactcg gcacttctcc ttccccggtg acgagtacat
     2881 cctccacgac ctgtacaacg acggcccaag cgaactggcc ttcctcaccg tggaacacaa
     2941 gagcaatgcg aaacaggact gatgaactat gcgagaagga aaatcatgaa ggttcttgtc
     3001 accggcgcca cgggctacct aggtgcggtc gccgccgagg cgctcgccgc acgaggtcat
     3061 caagtcttgg gacttgcccg atccgagcgg tccgctagtg cactgcgcga gcgcggcctc
     3121 gaacccgtca tgggcgactt cggcgacccg gtgagcctgg ccaacgccgt tcacgacgcc
     3181 agacccgacg tcgtggtgag caccgccagc gtcggcgggg cgagcgggga ccaagccgcc
     3241 ttcgcccggg acggtgaggc ggtacgcgcc atgcgggaag cgcttgctga tcacggcggc
     3301 gctctggtct tcaccagtgg gtccgcggtc ttcggggtct tcaacggcgg cgatgccacc
     3361 gacaccgtct acgacgaaga cacccgctta ccgcttccgg catcggtttt cgcgcccgat
     3421 tccgcgggcg tgcacccgct gctggccgcc ggtttcgggg cggcgatggc cgctcgcgtc
     3481 gagaccgaac gggccgtcct ggccgacggg gacgtgcgcg gtgtggtggt acgacccggg
     3541 ttggtctacg gacgcggcgg caattcggac cttcggtcct tgatcgaccg ggcgcggaag
     3601 gagggccatg ccggtcactg gggaagcggt ggcacaactc agagctacgt gcacgtcgac
     3661 gatttggccg agttattcgg cctggcagtc gaactcgcgc cgcacggggc gatcctgcac
     3721 ggcgtcgccg gcgacgtcac ccagcgcgac ttggcccgcg ccatcaaccg gatgatcgga
     3781 acagacgagc acaccgacag cctctcgctg ctgcagatgc ttggcatcga cgccgacaca
     3841 gtcgggaaca tcggcgcatc attcacaccg ccgcccagcg cagcatcggg tatcagcctg
     3901 tcgctgaaca aacggctctc ctcggacaag acgcgcaagc tcctcgactg gtcgcccaaa
     3961 cgcaccgacg tcggcgacga catcgccttc ggctcatacg cgaacgcata gccggaccgg
     4021 tgcgcgcagt gatccctaca cgctgacgaa gtatggagta tcgatgaccg acatcaaccg
     4081 caccaccgtc cgcatcccga cgccgtctgg gttcgatatc gaggcctggt tctacctacc
     4141 cgaggggccg gggccgtttc cggcggtggt gatgggccat ggactcggtg ccgtcaaggt
     4201 cggcggactg gcatcgttcg ccgaacgatt ctgccgggaa gggttcgccg cggtggtcat
     4261 cgactaccgc caatggggcg gctcggaggg gcagcaaccc cgcgacgaac tgaacgttcc
     4321 acgccaacgc gacgactaca gcgctgcgat cgggtgggcc gcggcccaac cgaacatcga
     4381 cgaacaccga atcttcgtgt ggggcacctc atttgccggc atgcacgcca ctgcactggc
     4441 cgcctcagat caccgcctgg ctggagcgat cgcccaatgc ccgctggttg acggtcttgc
     4501 cggttcacgg ctggtgcgac ccacccgttc ccttgcgcta ttcgccacgg cgatcatcga
     4561 tcgcctcggg tcattccttg gccgcgcgac gatctatctg ccggtcaatg tggcgcccgg
     4621 cgaatggggc atcgccgaca catcggacgc gctgttcggc aagcaagtca tgcacccgcg
     4681 cgagccgatc gactggcaca accggatggc agcacggtca ttgctgagca tcccggccca
     4741 ccgtcccgtc cgccacgccc gcgacatcaa catcccgatc ctgctcatcg tcgccgaaca
     4801 cgacacccag gcaccggtcg gtcccgcact cgcaatggcc gatcgcgcac cccgagcgga
     4861 gcttcaccgc agcacaggcg ggcactacga cgtctacgac ggtggcgccg ccttcggtga
     4921 cgtcatcgag tgggaagtcg acttcctccg acgccacgcc cgtatcgagc caaccgcacc
     4981 ccagacaccc tcggattcaa gctcaacgcc caaaccctaa acgcccgcgg cccccgccca
     5041 taacgtcggc cattggcgcg gacag
//
LOCUS       DASRJA010000651         8172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1839362,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000651 DASRJA010000000
VERSION     DASRJA010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8172
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839362"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFC01_29255"
     CDS             <1..222
                     /locus_tag="VFC01_29255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZN83741.1"
                     /translation="DPVEVTGTSIELSYSDGDLWDWAVSWDDWVAMSALSPQSSPSEI
                     PSTAPATPTDAPQPVNGRPGGQSATRLPR"
     gene            complement(176..940)
                     /locus_tag="VFC01_29260"
     CDS             complement(176..940)
                     /locus_tag="VFC01_29260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN83742.1"
                     /translation="MTRPTASERVAVVTGASAGIGEATAKTLAAQGFHVVCVARREGP
                     INALAEQIGGTAIVADVTSDEDVTALASALDRVDVLVNNAGGARGLEPVAEADLDNWR
                     WMWEANVLGTLRVTRALLPKLIESGDGLIVTVTSIAAVEIYDGGSGYTAAKHAQGVLH
                     RTLRSELLGKPVRLTEVAPGMVKTDFSLRRFEGDEERAAKVYEGVTPLVAEDIAEVIG
                     FVASRPSHVDLDLIVVRPRDQVSGASGSRFDRQVAR"
     gene            1205..2449
                     /locus_tag="VFC01_29265"
     CDS             1205..2449
                     /locus_tag="VFC01_29265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZN83743.1"
                     /translation="MLARPHVVAPSLKVADAAAASVFGAARLRGPIARDAIAQVTQLS
                     IATVNRQVTALLDAGILRERADLAVSGAIGRPRVPVEVNHEPFLTLGVHIGARTTSIV
                     ATDLFGRTLDAVETPTPRGTQAAALASLAGSARRYLSRWHRRRALWVGVAAGGVVDGS
                     TGYLDHPRLGWSAAPVGPVLAETLGLPVSVASHVDAMAGAELLLGVRKLTGSDVARAG
                     APAPAPTSLYVYARETVGYALSIGGRVHSPASGPGTIAALPAHSELLGGTGQLESTVS
                     DEAVLIAARKARIIPAEGPASTVTAVLRAARQGNQQARALLAERARVLGEAVALLRDM
                     LNPDDLVVGGQAFTEYPEGMNLVEAAYTERSVLPARDIRVTVFGNRVQEAGAGVVSLG
                     GLYADPIGAMRRAQSRRPEATA"
     gene            2600..3472
                     /locus_tag="VFC01_29270"
     CDS             2600..3472
                     /locus_tag="VFC01_29270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83744.1"
                     /translation="MQTIGWPFRAEEALEDGALTFRELRRFHQAVYPGVWAPRGVELS
                     ATDRAKAAWLWSRRDGVIAGLSASAMLGAKWIDDDLAVELIHTNRRSPPLITVHTDEL
                     LSGETQTIRGLPVTAPARTAFDLGRRLSLKDGVQRIDALMNATDVKVDNIETVAGTHP
                     GVRGLRQLRQTLDLVDGGAESPYESLTRLLLVQAGFPRPQTQIRVLDEDGCVIARIDM
                     GWRKYLVGVDFEGAHHWTDPKSRTWDIERYHLLPELGWTDIRLTSGILHNTPQRFLDR
                     VGAALIARGCPRTW"
     gene            3581..4885
                     /gene="mshA"
                     /locus_tag="VFC01_29275"
     CDS             3581..4885
                     /gene="mshA"
                     /locus_tag="VFC01_29275"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HZN83745.1"
                     /translation="MRLATDLPRRVAVLSVHTSPLAQPGTGDAGGMNVYVLQSALQMA
                     RRGVEVEIFTRATTSTDQPVVKVAPGVLVRNVVAGPFEGLDKNDLPTQLCAFTAGVLR
                     AEATYEPGYYDIVHSHYWLSGQVGWLARDRWAVPLVHTAHTLAAVKNAALADGDSPEP
                     PLRAVGEQQVVDEADRLIVNTEHEAHQLVSLHHADPARIDVVHPGVDLGMFTPGDKRA
                     ARAALGLDVNESVLAFVGRIQPLKAPDVLLSAAAKLPGVRVLVAGGPSGSGLAAPDGL
                     LRLADELGIADRVTFLPPQTRDQLVDVYRAADLVAVPSYSESFGLVAVEAQACGTPVV
                     AAAVGGLPVAVRDGVSGTLVDGHDVDDWAAAIDALLRRGPETMRAAAVEHASTFSWTH
                     TVDALLASYSRAIGDYRARHPRRDATARRNGRRFSMRRGVRA"
     gene            4882..5379
                     /locus_tag="VFC01_29280"
     CDS             4882..5379
                     /locus_tag="VFC01_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HZN83746.1"
                     /translation="MSPVKKLIEDALDERELTFTHHAKGLIVELPGERRLKTPTMLTI
                     GEHSVRIEAFVCRRPDENTESVYRFLLKRNRRLYGVAYTLDNIGDIYLVGRIPLESVT
                     ADEIDRLLGQVLEAADSDFNTLLELGFRSSIQKEWEWRVSRGESLKNLEAFAHLIDED
                     DTGAS"
     gene            5393..6148
                     /locus_tag="VFC01_29285"
     CDS             5393..6148
                     /locus_tag="VFC01_29285"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HZN83747.1"
                     /translation="MASDSSATLILLRHGESDWNEKNLFTGWVDVDLTEKGRAEAVRA
                     GELIKELDRQPDVLYTSLLRRAINTANLALDTADRHWIPVHRNWRLNERHYGALQGLN
                     KAETKEKYGDDQFMAWRRSYDTPPPPIERGSKYSQDTDPRYADINGGPLTECLKDVVE
                     RFVPYYEQTIVPDIRAGKTVLIAAHGNSLRALVKYLDGMSDEDVVGLNIPTGIPLRYD
                     LDGDLKPLVIGGEYLDPEAAAAGAAAVAAQGAK"
     gene            6279..7457
                     /locus_tag="VFC01_29290"
     CDS             6279..7457
                     /locus_tag="VFC01_29290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN83748.1"
                     /translation="MSVVSALLLVGVVALLALAIGVGLGASLTPRILERRQRRATEQS
                     GLTVSQMLEHIVSGSPVGIVVVDTYRDVVYTNDRARELGLVRDRLLDDRAWLAAERTL
                     ATGEDREVDLSPRKRANPGRSGLSVRGHVQLLTEQDRRFAVVYVDDQSEHARMEATRR
                     DFVANVSHELKTPVGAMGVLAEALLASADDPDTVRRFAEKMLTESNRLANMVGELIDL
                     SRLQGGERLPDLEAVDVDRVVAEALSRYKVAADNADIAITTDAPTGYRVMGDESLLVT
                     AIANLVSNAIAYSPNGSSVSISRRRRGSNIEIAVTDRGIGIAREDQERVFERFFRVDK
                     ARSRATGGTGLGLAIVKHVAANHNGSIRLWSQPGTGSTFTLSIPAYPDHEDESDERED
                     "
     gene            7466..8152
                     /gene="regX"
                     /locus_tag="VFC01_29295"
     CDS             7466..8152
                     /gene="regX"
                     /locus_tag="VFC01_29295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensory transduction protein RegX"
                     /protein_id="HZN83749.1"
                     /translation="MTSVLIVEDEESLADPLAFLLRKEGFEATVVADGPAALAEFERA
                     GADIVLLDLMLPGMSGTDVCKQLRSRSSVPVIMVTARDSEIDKVVGLELGADDYVTKP
                     YSARELIARIRAVLRRGSDNDDSGIGDGILEAGPVRMDVERHVVSVNGEQITLPLKEF
                     DLLEYLMRNSGRVLTRGQLIDRVWGADYVGDTKTLDVHVKRLRSKIEADPANPVHLVT
                     VRGLGYKLEG"
BASE COUNT         1338 a         2737 c         2783 g         1314 t
ORIGIN      
        1 gacccggtcg aggtcaccgg cacctcgatc gagctgtcct actccgacgg ggacctctgg
       61 gactgggccg tcagctggga cgactgggta gcaatgtcgg cgctgtcgcc acagagctca
      121 ccgagcgaga tcccgagcac agcgcctgcc acgccgacgg acgcgcctca gccggtcaac
      181 gggcgacctg gcggtcaaag cgcgacccgc ttgccccgct gacctgatcg cgcggcctga
      241 ccacgatcag atccaggtcg acatgcgacg gccggctggc aacgaatccg atcacctcgg
      301 cgatgtcctc ggccaccagc ggggtgacgc cttcatacac cttcgcggcg cgctcctcat
      361 cgccttcgaa ccggcgtaac gagaagtcgg ttttcaccat tccgggcgca acttcggtga
      421 gccgcaccgg ttttccgagt agctcgctgc gcagcgtgcg gtgcagaaca ccctgggcgt
      481 gtttggccgc cgtgtacccg gagccaccgt cgtagatttc gaccgccgcg atcgaggtga
      541 ccgtgacgat cagtccgtcg ccggactcga tcagcttggg cagcaatgcg cgggtgacgc
      601 gcagcgtgcc cagcacgttg gcctcccaca tccagcgcca attgtcgagg tccgcctcgg
      661 ccaccggctc cagcccgcgg gccccgccgg cgttgttgac cagcacatca acccggtcca
      721 gggcgctggc cagcgcggtt acgtcctcgt cggaagtgac gtccgccaca atcgcggtgc
      781 cgccgatctg ctcggcgagt gcgttgatgg ggccctccct gcgggccaca caaacgacgt
      841 gaaagccttg ggctgcaagc gttttcgcgg tcgcttcacc gatgccggca ctggcgccgg
      901 tgaccaccgc gacccgttcg gaggctgttg gtctcgtcat ctgcccaact ttagttggcg
      961 tgctaagttc accgtgtgta ccggaatggt caggctgcag tcgccacgcg gcactgttgt
     1021 tgttgcgcac ttcgccgcgc ctgactgata ccggacgttt cctgtcctac tgagtcgcca
     1081 ggtgtggcac ggacccacca gccgacttcg acaaaggaca gatcccgtgc gcacttcctc
     1141 caccaccacc gcgctccgcc agccggcggc ttccgccgtt tcggccgccc acaccaagcg
     1201 ggcgctgctt gcgaggcccc acgtcgtcgc gccctcgctg aaggtcgccg acgcggcggc
     1261 ggcctcggtg ttcggcgcgg cgcgcctgcg cgggccgatc gcccgtgacg ccatcgccca
     1321 agtcacccag ctgagcatcg cgacggtgaa ccgccaggtg accgcgctgc tggacgccgg
     1381 gattctgcgt gagcgcgccg acctcgcggt ctccggcgcc atcggcaggc cgcgtgtgcc
     1441 ggtcgaggtc aaccacgagc cgttcctgac gctgggcgtc cacatcggtg cgcgcacgac
     1501 cagcatcgtg gccaccgacc tgttcggccg cacgctggat gcggtggaga cgcccacccc
     1561 gcgcggcacc caggccgcag cgttggcctc gctggccggc agtgcccgcc gctatctgag
     1621 ccgctggcac cgccgccgcg cgctgtgggt cggggtggct gccggtggcg tcgtcgacgg
     1681 cagcaccgga tacctcgacc acccgcggct gggctggtcc gccgcgccgg tcggcccggt
     1741 gctggccgag acgctcggcc tgccggtgtc ggtggcctcg cacgtcgacg cgatggcggg
     1801 cgccgagctg ctgctcgggg tgcgcaaact gactggatcc gatgtcgccc gcgccggggc
     1861 tccggcgccg gcgccgacca gcctgtacgt gtacgcccgc gaaaccgtcg gctatgcgct
     1921 gtcgatcggc gggcgggtgc actcgccggc cagcggtccg gggaccatcg cggcgctgcc
     1981 cgcccactcg gaactgctcg gcggcaccgg ccagctggaa tccaccgtca gcgacgaggc
     2041 ggtgctgatc gccgcccgca aggcccgcat catcccggcc gagggtccgg cgtcgacggt
     2101 gacggcggta ctgcgggcgg cccgacaggg caatcagcag gcgcgcgcat tgctggccga
     2161 gcgggcccgg gtgctcggag aagcggtcgc gctgctgcgc gacatgctca accccgacga
     2221 cctcgtggtc ggtggccagg cgttcaccga gtaccccgag ggcatgaatc tcgtcgaggc
     2281 ggcgtacacc gaacggtcgg tgctgccggc ccgcgatatt cgcgtcaccg tgttcggcaa
     2341 ccgggtgcag gaagcgggcg ccggagtggt gtcgctcggc ggcctctacg cagacccgat
     2401 cggggcgatg cggcgagccc agagccgtcg cccggaagcc actgcctgac tgaccgctcg
     2461 gcggccgccg cgagtgtgcg gtttcatacg caacacgctg cggtgggcgt atcgatatgc
     2521 acagtcgcgg actcgtgtcc tggggataac ccttcgaagt gtcggctctg ctgtcggatc
     2581 gcgatgcgac cgtcacggca tgcagacgat cggctggcca tttcgagcag aagaagcact
     2641 cgaagacgga gcgctgacgt ttcgcgagct acgacgcttc catcaggcgg tctatccggg
     2701 agtgtgggcg ccgcgcggag tggagttgtc ggcgaccgac cgtgccaaag cggcgtggct
     2761 gtggtcgcga cgcgacggcg taatcgctgg cctgtctgcc tcagcgatgt tgggagccaa
     2821 gtggattgac gacgacctgg cggtcgagct catccacacc aatcggcgat caccgccgct
     2881 aatcacggtc cacacggacg aattgctctc tggcgagaca cagacaattc gtggactgcc
     2941 cgtgacggca cctgcgcgga ccgcgtttga cctcgggcgg cgcctgagcc tgaaggacgg
     3001 tgtgcagcgc attgacgcgt tgatgaacgc gaccgatgtc aaggtcgaca acattgagac
     3061 cgtcgcgggg acacatcctg gcgtgcgagg ccttcggcag ttacgccaga cgttggattt
     3121 agtggacggt ggcgccgagt cgccctacga atcgctcact cgcctgctcc tcgtgcaggc
     3181 cggcttcccg cggccgcaga cacaaatccg agtgctcgac gaggacggct gcgtcatcgc
     3241 gcgaatcgat atgggctggc ggaaatatct cgtgggcgtc gacttcgagg gcgcccatca
     3301 ctggaccgat ccgaagagcc ggacgtggga cattgagcga taccacctgc tcccagagct
     3361 cggctggact gacatcaggc tgacgagcgg gatactgcac aacactccgc aacggtttct
     3421 tgatcgcgtg ggtgcggctc ttatcgcccg cggctgcccg aggacgtggt gactgtgcgt
     3481 atccatacgg cgtcgtcggc ctgtcgcgta tgaaaccgca cactcgccac accagtgcag
     3541 gcctgacgag cccgcggaac cacggtgtag acatgtctgt gtgcgcctag ccacggatct
     3601 gccccgccgc gtcgctgtgc tatcggtgca cacctctcca ctggcccagc cgggcaccgg
     3661 cgatgccggc ggtatgaacg tctacgtgct gcaaagtgcg ctgcagatgg cgcgccgcgg
     3721 cgtcgaggtc gagatcttca cccgcgcgac aacctcgacc gatcagccgg tggtgaaggt
     3781 ggcgcccggc gtgctggtgc gcaatgtggt ggcggggccg ttcgaggggc tcgacaaaaa
     3841 cgacctgccc acccagctgt gcgccttcac cgcgggcgtg ctgcgcgcgg aggccaccta
     3901 cgagcccggc tactacgaca tcgtgcattc gcactattgg ttgtccggac aggtcggctg
     3961 gctggcccgg gatcgctggg cggtgccgct ggtacacacg gcgcacacgc tcgccgcggt
     4021 gaagaatgcc gcgctggccg acggcgactc gcccgaaccg ccgttgcgcg ccgtcggcga
     4081 gcagcaggtc gtcgacgagg cagaccggtt gatcgtcaac accgaacatg aagcgcacca
     4141 actggtttcg ctgcaccatg ccgacccggc ccgcatcgac gtggtgcatc ccggcgtcga
     4201 cctgggaatg ttcacgccgg gggacaagcg cgccgcgcgc gccgcgctgg ggctcgacgt
     4261 caacgagtcg gtgctcgcgt tcgtcggccg catccagccg ctcaaggctc ccgacgtgct
     4321 gctgagcgcg gcggccaaac tgccgggcgt gcgggtgctg gtggccggtg gcccgtcggg
     4381 cagtggactc gccgcgccgg acggattact gcggctcgcc gacgaactgg gcatcgccga
     4441 cagggtgacg ttcctgcccc cgcagacccg cgatcagctt gtcgacgtct accgggctgc
     4501 cgatctggtc gcggtgccga gctattcgga gtccttcggg ctcgtcgccg tcgaggcgca
     4561 ggcgtgcggc accccggtgg tggccgccgc agtcggcggg ctgccggtcg cggtgcgcga
     4621 cggggtcagt ggcaccctcg tcgacggcca tgacgtcgac gactgggccg cggcgatcga
     4681 cgcgctgctg cggcgcggcc ccgaaaccat gcgcgcggcc gccgtcgagc acgcctcgac
     4741 gttctcgtgg acgcacaccg tggacgcgct gctggccagc tacagccggg cgatcggtga
     4801 ctaccgggcc cgccacccac gtcgggatgc gacggcgcga cgcaacgggc gccggttctc
     4861 gatgcggcgc ggagtgcggg cgtgagcccc gttaagaagc tcatcgaaga tgcgctcgac
     4921 gaacgcgagc tgacattcac ccatcacgcc aagggcctga tcgtcgagtt gcccggtgag
     4981 cgtcggctca agacgcccac catgctcacc atcggcgagc attcggtgcg gatcgaggcc
     5041 ttcgtctgcc gcaggcccga cgagaacacc gagtccgtgt accggttcct gctcaagcgc
     5101 aaccgccggc tgtacggcgt ggcgtacacg ctggacaaca tcggcgatat ctatctggtc
     5161 gggcgcatcc ctctggagtc ggtgacggcc gacgagatcg accgcctgct cggtcaggtg
     5221 ctcgaggctg cggactccga cttcaatacg ttgctggagt tgggttttcg gtcctcgatc
     5281 caaaaagagt gggaatggcg ggtatcccgc ggcgaatcgt tgaagaacct ggaggcgttc
     5341 gcgcacctga tcgacgaaga cgacaccggg gcgtcatgag aggatcggcg tcatggcttc
     5401 agattcgtcg gcgacgctga tcctgctccg ccacggcgag agtgactgga acgagaagaa
     5461 cctgttcacc ggctgggtcg acgtcgacct caccgagaag ggcagggccg aagcggtccg
     5521 ggcgggtgag ctgatcaagg agctggaccg ccagcccgac gtgctctaca cgtcgctgct
     5581 gcggcgggcg atcaacacgg ccaacctcgc gctggacacg gccgaccggc attggattcc
     5641 ggtacaccgc aactggcggc tcaacgaacg ccattacggc gcgctgcagg gtctgaacaa
     5701 agccgagacc aaggagaagt acggcgacga tcagttcatg gcctggcggc gcagctacga
     5761 caccccgccg ccgccgatcg aacggggcag caagtacagc caggacactg acccccgcta
     5821 cgccgacatc aacggcgggc cgctgaccga atgcctcaag gacgtcgtcg agcggttcgt
     5881 gccgtactac gagcagacga tcgtccccga tatccgggcc ggcaagacgg tgctgatcgc
     5941 cgcccacggc aactcgttgc gtgcgctggt gaagtatctc gacggcatgt ctgacgagga
     6001 cgtcgtcgga ttgaacatcc cgaccggcat cccgctccgc tacgacctgg acggtgacct
     6061 caagccgcta gtcatcggcg gcgagtatct ggaccccgag gcggctgcgg caggtgcggc
     6121 cgcagtcgcg gcccagggcg cgaagtaagg cgctcgacgg tgccgcacgg caaacactgg
     6181 gtaaacggct atcgaactcc tgcgattggc cgtgtgacat gtcccgtttt agccgcacgc
     6241 tgctgggatg acgttcaccg gatgcgtacg attttcgcgt gagtgtggta tcggcgctcc
     6301 tacttgtggg ggtcgtggca ctgctcgctc tggcgatcgg tgtcgggttg ggggcgagcc
     6361 tcaccccgcg catcctcgaa cgcaggcaac gccgcgccac cgagcagtcc ggtctgaccg
     6421 tctcgcaaat gctcgagcac atcgtctcgg gttcgccggt ggggatcgtc gtggtcgaca
     6481 cctaccgtga cgtcgtctac accaatgacc gggcccgcga gctggggctg gtccgtgacc
     6541 ggctgctcga cgaccgcgcc tggctggctg cggagcgcac gctggcgacc ggcgaggacc
     6601 gcgaggtcga cctgtcgccg cgcaaacgcg ccaatcccgg ccggtccggg ttgtcggtcc
     6661 gcgggcatgt gcagctgctg accgaacagg accgccggtt cgcggtcgtg tacgtcgatg
     6721 accagtccga gcacgcaagg atggaagcga ctcgcaggga tttcgttgcg aacgtcagcc
     6781 acgaactgaa aacgccggtc ggcgcgatgg gcgtgctggc cgaggcgctg ctggcctccg
     6841 ccgacgaccc cgacaccgtg cgccggttcg ccgagaagat gctcaccgaa tcgaaccggc
     6901 tggccaacat ggtcggcgaa ctgatcgacc tgtcgcggct gcagggcggc gaacggctgc
     6961 ccgacctgga ggccgtcgac gtcgaccgcg tcgttgcaga ggcgctctcg cgctacaagg
     7021 tcgccgccga caatgccgac atcgcgatca ccaccgacgc cccaaccggc taccgcgtga
     7081 tgggtgacga gtcgctgctg gtgaccgcga tcgccaactt agtgtccaat gcaatcgcct
     7141 actcgcccaa cggatcttcg gtgtccatca gccgccgtcg gcgcggaagc aacatcgaga
     7201 tcgccgtgac cgaccgcggt atcggcatcg cgcgcgaaga ccaggagcgg gtgttcgaac
     7261 gcttcttccg cgtcgacaag gcacggtcgc gggccaccgg cggcaccggc ctgggcctgg
     7321 cgatcgtcaa gcacgtcgcc gccaatcaca acggatccat ccggctgtgg agtcagccgg
     7381 gaacgggatc gacgttcacc ttgtcgattc cggcctaccc cgatcatgaa gatgaatcgg
     7441 acgagcgaga ggactagcaa gaccaatgac cagcgtgttg atcgtggagg acgaggagtc
     7501 cttggccgat cccctggcct ttctgcttcg caaggagggc ttcgaagcca ccgtggtggc
     7561 cgacggcccc gcggccctgg ccgaattcga gcgcgccggc gctgacatcg tcctgctgga
     7621 cctgatgctg ccgggaatga gcggaaccga cgtgtgcaag caattgcgtt cgcgctcaag
     7681 cgttcccgtc atcatggtga cggcgcggga cagcgaaatc gacaaggtcg tagggctcga
     7741 actcggcgcc gacgattacg tcaccaagcc gtactcggcc cgcgagctga tcgcccggat
     7801 tcgggcggtg ctgcgccgcg gcagcgacaa cgacgactcc ggtatcggcg acggcatttt
     7861 ggaggccggg ccggtacgga tggacgtcga gcggcacgtg gtatccgtca acggcgagca
     7921 gattacgttg ccgctcaagg agtttgacct gctggagtac ttgatgcgca acagcggccg
     7981 ggtgctcact cgcggacagt tgatcgatcg ggtatggggc gccgactatg tcggcgacac
     8041 caagacgctc gatgtgcatg tcaagcgcct gcgcagcaag atcgaggccg acccggcaaa
     8101 cccggtgcat ctggtcaccg tgcgcgggct cggctacaag ctcgaagggt aagtttctgc
     8161 ggcgaacgtg gg
//
LOCUS       DASRJA010000652         9283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1582083,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000652 DASRJA010000000
VERSION     DASRJA010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9283
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582083"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFC01_29300"
     CDS             <1..454
                     /locus_tag="VFC01_29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HZN83750.1"
                     /translation="GAVAVPGVWADDATVVVCCTLSQPASAATVASLITYEQLVSAAL
                     ITTAAENASAIADIITVLQSRGAIEQAKGALMGLVRCDADQAWKMLRRGSQEFNVKLR
                     ELSVAIVEHISGAPAEQPGAETPITPDERTREAARLMWAALSEAPLPQ"
     gene            complement(443..1537)
                     /locus_tag="VFC01_29305"
     CDS             complement(443..1537)
                     /locus_tag="VFC01_29305"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HZN83751.1"
                     /translation="MTPIRYTAPGRINLIGEHTDYNLGFALPIALPERTVVTFLPDDG
                     DAVGVRSDQEDGDVRIPLDTAPGDVPGWAGYVAGVVWALREAGHHISGGTMAIASDVE
                     MGSGVSSSAALECAVLGALTTATGLRIDRIELARIAQRAENDYVGAPTGMMDQLASLC
                     GEPRKALMIDFREPIVRPVAFDPDASDVALLLVNSRAPHQHAGGEYAARRASCERAAA
                     DLGVSSLREVQDRGVAALAAVSDPVDARRARHILTDNQRVLDVVVALEKSDYSAIGRL
                     FNESQASMRDDFEITTEHIDLIADAAVQSGALGARMTGGGFGGCVIALAPAAKTEHIA
                     DSIRKAVAAAGYNAPTISRTRAGQGATLLR"
     gene            complement(1677..2771)
                     /gene="galT"
                     /locus_tag="VFC01_29310"
     CDS             complement(1677..2771)
                     /gene="galT"
                     /locus_tag="VFC01_29310"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HZN83752.1"
                     /translation="MTDPATRWPLADGRELLLFALPGHTPAPVPDERPLPERGNQQSE
                     LRFDRQTGQWVIIAALRQDRTYKPPPDQCPLCPGPSGLTSEIPAPSYDVAVFENRFPS
                     LSGASSAPFHLPAVGGDLFLSAPGHGRCEVICFSSDHTGSFSQLELPHARLVVDAWRH
                     RTADLMARPGIAQVFCFENRGEEIGVTLTHPHGQIYGYPYLPPRAAVMLREARAYADR
                     TGGNLFTDILAREVADGSRIIARTELFTAFVPFAARWPVEVHIYPNRFVHNLVDLTDA
                     ELDGFAQMYLDVLSRFDRMYPTALPYISALHQYANTSEQREGYFHTELMSVRRSATRL
                     KYLAGSESAMDAFISDVTPESVAQRLRELA"
     gene            complement(2768..3556)
                     /locus_tag="VFC01_29315"
     CDS             complement(2768..3556)
                     /locus_tag="VFC01_29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HZN83753.1"
                     /translation="MLAAERRYAILRALNAGGAVRVADLAAELDVSEMTVRRDLDALD
                     AQNLLRKVHGGAVTRHNRGEEPGSSVKAAQQRAEKAAIAAVAAETVEDGMTIAISAGT
                     TTTELARRLRRRSSITVVTNSISVFEVLTDPAADAADAPQVYLSGGVRTPSDALVGPI
                     ADAAIGSFRVDGTYLGVHGFDPEAGLTTPNIAEAQTNRTLIGIGAQLTVLADHTKYRE
                     VGTNVFARMRRVDNLIVDDGLTIGDRGIIEQHVGRLTIAPVERQ"
     gene            3670..5385
                     /locus_tag="VFC01_29320"
     CDS             3670..5385
                     /locus_tag="VFC01_29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZN83754.1"
                     /translation="MTTTTTTLAAENVLRLNLGWLDYVLVAIYFVFVLGIGFIARSQI
                     STSLDFFLSGRRLPAWVTGLAFISANLGAVEIMGMSANGAQVGLSTMHYYWIGAIPAM
                     LFLGVVMMPFYYGSKVRSVPEFMRRRFGPGAHLVNAISFATAQVLIAGVNLFLLATVI
                     NALLGWPRWISLIVAALVVLSYITLGGLSAAIYNEVLQFFVILAALVPLTVFGLIRIG
                     GWNGLKDKVIDTVTDGKVTASVHEQVTTWPGQALSGFESPVWSVIGIVFGLGFVLSFG
                     YWTTNFVEVQRAMASSSISAARRSPIIGSFPKMFVPFVIIVPGMIAAAAIGDMIRLKT
                     TGEGDITYNDAMLLMIRDILPNGLLGVAVAGLLASFMAGMAANISAFNTVFSYDIWQD
                     YVVKDRPDNYYILVGRIATIGATILAIFTALIAAGFSNLMDYLQTLFGFFNAPLFATF
                     ILGMFWKRMTSTAGWVGLVSGTLSAIFIFILSKVGVFSLPGQGVPFVAASVAFVVDII
                     VSVAVTMVTTPKPANELVGLVYSETPREAFHDPESVGKAWYYRPVPLAAVSLAMVIVL
                     NIVFH"
     gene            5398..5682
                     /locus_tag="VFC01_29325"
     CDS             5398..5682
                     /locus_tag="VFC01_29325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83755.1"
                     /translation="MRHLFDVRNIIGALLGIYGVVLTIAGFAPGLLGDHHSGATDNPV
                     DLYVGTDANWWVGLALIVVAIVFIGWALLRPVEVPETPPQATDSEQPQVE"
     gene            complement(5670..6563)
                     /locus_tag="VFC01_29330"
     CDS             complement(5670..6563)
                     /locus_tag="VFC01_29330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018603146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZN83756.1"
                     /translation="MVGHGLVVLLAVLAAVFLAIGIVVRQRATMDVPQEHGVSSVMFL
                     TLLRRPLWWAGTGAAVAGYVFQALALAKGSLLLVQPILVSALLFALPLSARLAHRRVT
                     RGEWVWAVVLTTALAVFVVLAKTRPGDYEASLTTSTLVAVICTAAVCACVIVATRTMG
                     WKRAVLLAFAVGVLFGVVAVLTKLVMHVLTHEGVRAVFATPVVYLLAVIAVFATFLQQ
                     SAFHAGSLQTSVPTMLVLEPVIAVLLGAVVLGENLDVSHLDAVVITISTVAMAAATIA
                     LGRDEGAYEEQLEAAVVRHST"
     gene            6691..7032
                     /locus_tag="VFC01_29335"
     CDS             6691..7032
                     /locus_tag="VFC01_29335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZN83757.1"
                     /translation="MNVRRIILLVGAVVLLVGVIGLLMPVSVPGPDNGSIGCGNGLVQ
                     DSSAAREADNNNPVNLPILNEIIPHTDYVTQCQSAVSGRRAWTIPVAVVGLVVLAGSF
                     FVGGRAGVRTP"
     gene            complement(7036..7311)
                     /locus_tag="VFC01_29340"
     CDS             complement(7036..7311)
                     /locus_tag="VFC01_29340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83758.1"
                     /translation="MKRQVLPYVTVEVDDRLRRRLRRAGERLRVNVRAYLLSAADDVD
                     AAGDKVRDLCDRAVNRVDSVVATVNDRLGADEPKQLQLHLVSSREAS"
     gene            complement(7374..7766)
                     /locus_tag="VFC01_29345"
     CDS             complement(7374..7766)
                     /locus_tag="VFC01_29345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83759.1"
                     /translation="MKKLGVYAITAVGATAVSMAFFGSGVAAADDYSGQTYGDASKAI
                     SDAGKKAVIASRAGDTLTDDNCIVTHSQSAPWIKGDDFAPVTDTVLVYLNCNATVATA
                     KDPGNSAASPEGKAAMQKAQEDKSSGSG"
     gene            8046..8318
                     /locus_tag="VFC01_29350"
     CDS             8046..8318
                     /locus_tag="VFC01_29350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83760.1"
                     /translation="MRSGLTARRLAATAGIGAIIAMGALSACAKEEKAPETTTPTTTT
                     TTTTSEATPAPPPPAPTEKSINPTGGNLFTPPVKAPPAPTGVPGDN"
     gene            complement(8359..9003)
                     /locus_tag="VFC01_29355"
     CDS             complement(8359..9003)
                     /locus_tag="VFC01_29355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN83761.1"
                     /translation="MPQQTDRATRPKLTSRGAATRNRIVLAAAELMHVQGVAATTIDD
                     VLAASASSKSQFYQHFDDKSELVYEVITMRADEILSWQRLRLEKVDSFRGLYHWRDAM
                     VQRCALRRGLWGCELGSLAAELSDTDDKARASLSEHFTEWRGLLAAAVERMRDNGALV
                     ADVDAKALATGLLAAVEGGYLLSQTGRDPRLMQSALNMAIGHVASFATAGTALR"
     gene            complement(9025..>9283)
                     /locus_tag="VFC01_29360"
     CDS             complement(9025..>9283)
                     /locus_tag="VFC01_29360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83762.1"
                     /translation="RYSGFSIGYNVCVAAFGGTTPYAVTWLTAETGNNVAPAYYVVVA
                     AIVSLLTILTIRETARRPLPQGVADEVFQSGTDVRGAATAG"
BASE COUNT         1551 a         3074 c         2989 g         1669 t
ORIGIN      
        1 cggcgccgtg gcggtgcccg gtgtctgggc cgacgatgcg acggtcgtcg tgtgctgcac
       61 gctcagccag cccgccagcg ccgcgaccgt agcgagcctg atcacgtacg aacaactggt
      121 ctccgcagca ctgatcacca ccgcggccga gaacgcctcg gccatcgcgg acatcatcac
      181 ggtcctgcag tcccgcggcg ccatcgagca ggcgaagggc gcgctgatgg gcctggtccg
      241 ctgcgacgcg gaccaggcgt ggaagatgct gcgccgcggc agccaggagt tcaacgtgaa
      301 gctgcgggag ctctccgtgg cgatcgtcga gcacatcagc ggcgctcctg ccgaacagcc
      361 cggcgccgaa acgccgatca cacccgacga gcgcactcgc gaggccgccc gcttgatgtg
      421 ggccgcgctg tcggaggctc cgctaccgca gtagtgtcgc gccctggccg gcccgggtgc
      481 ggctgatcgt cggggcgttg taaccagcgg cggcaacggc tttccgtatc gagtcggcga
      541 tgtgctcggt tttcgctgcg ggcgcgaggg ctatcacgca gccgccgaac ccgccgccgg
      601 tcatccgcgc acccagcgcg cccgactgca ccgcggcgtc ggcgatcaga tcgatgtgtt
      661 cggtggtgat ctcgaagtcg tcgcgcatcg acgcctgcga ctcgttgaac agtcggccga
      721 tcgcggagta atcggacttt tcaagcgcga caacgacatc cagcacgcgt tggttgtcgg
      781 tcaggatgtg gcgggcacgc cgcgcatcga cagggtcgga caccgcggcg agagccgcca
      841 cgccgcggtc ctgcacctct cgtagcgacg acaccccgag gtcggccgct gcccgttcgc
      901 acgacgcccg gcgcgccgca tactctccac ccgcatgttg gtgtggcgca cgcgaattga
      961 caagcagcag cgcgacatcg gaagcgtcag ggtcgaacgc caccggtcgc acgatcggct
     1021 cccgaaagtc gatcatcagc gccttgcgtg gttctccgca cagcgacgcg agttggtcca
     1081 tcattccggt gggtgcgccg acataatcgt tctcggcccg ctgcgcgatg cgcgccagct
     1141 cgatccgatc gatgcgaagg cctgtcgcgg tcgtgagcgc gccgagtacc gcgcactcca
     1201 gtgccgccga agacgatacg ccggagccca tctcgacgtc gctggcgatc gccatggtcc
     1261 cgccggagat atggtggccg gcctcgcgta acgcccacac cacaccggcc acatagccgg
     1321 cccagccggg gacgtcgccg ggcgcggtgt cgagcggaat ccgaacgtcg ccgtcctcct
     1381 ggtcgctacg caccccgacg gcgtcgccgt cgtcgggtag gaaggtcacg accgtgcgct
     1441 cgggcagggc gatcggtagc gcgaaaccca ggttgtagtc ggtgtgttcg ccgatcaggt
     1501 tgatccggcc cggtgccgta tatctgatcg gagtcatccg ccgacctcgc aggctcggcc
     1561 ggcgcattcg gtgctgatga ttcgctcgca agcgtcatcc atgcctcgac tccccgctcg
     1621 ctccggtcct cgctcgcacc tcgctgcgat cctcactcgc ggtcgtcttc gggtgctcat
     1681 gcgagttccc gcaaccgctg cgcgacgctt tccggggtga catcgctgat gaaggcgtcc
     1741 atcgcggact cggaaccggc caggtacttc aacctggtcg cgctgcggcg caccgacatc
     1801 agctcggtat ggaagtagcc ttcacgctgt tcggatgtgt tggcgtattg gtgtagggcc
     1861 gaaatatagg gcagcgcagt gggatacatg cggtcgaacc ggcttagcac gtcgagatac
     1921 atttgcgcga agccgtcgag ctcggcgtcg gtcaggtcaa ccaggttgtg cacgaaccgg
     1981 ttcgggtaga tgtgtacctc gacgggccac cgggcggcga acggtacgaa cgcggtgaac
     2041 agctctgtcc tggcgatgat gcggctgccg tcggccacct cgcgggcgag gatgtcggtg
     2101 aacaggttgc cgcccgtgcg gtcggcgtaa gcgcgcgcct cgcgcaacat cacagcggca
     2161 cgcgggggca gatacgggta gccgtagatc tggccgtgcg gatgggtcaa cgtcacgccg
     2221 atctcctcgc cgcggttctc gaagcagaac acctgcgcga tgccggggcg ggccatcaga
     2281 tcggcggtgc ggtgtcgcca tgcgtcgacg acgaggcgcg cgtgcggcag ttcgagctga
     2341 ctgaacgagc cggtgtgatc gctggagaaa cagatcacct cacatcggcc gtgacccggc
     2401 gcggacagga acaggtcacc gccaaccgcc ggaaggtgga acggcgcgct gctcgcaccg
     2461 gacaggctgg ggaaccggtt ctcgaacacg gccacgtcgt agctgggcgc ggggatttca
     2521 ctcgtcaggc cggatggtcc ggggcacagc gggcattggt cgggcggcgg tttgtaggtg
     2581 cggtcctgac gcagcgccgc gatgatcacc cattggccgg tctgccggtc gaatcgcaac
     2641 tcggactgtt gatttccgcg ctctggcagc gggcgttcgt caggtaccgg cgccggggtg
     2701 tgccccggaa gcgcgaacaa caacagctct cgcccgtcgg ccagcggcca cctggttgcg
     2761 ggatcggtca ttgtcgttcc accggggcga tggtcagccg cccgacgtgt tgttcgatga
     2821 tgccccggtc tccgatggtc agcccgtcgt cgacgatcag gttgtccacc cggcgcatcc
     2881 gagcgaacac gttcgtgccc acttcgcggt acttcgtgtg atcggcgagc acagtgagtt
     2941 gtgcaccgat cccgatcagg gtccgattgg tctgcgcctc agcgatattc ggggtcgtca
     3001 ggccagcctc cggatcgaac ccgtgcactc cgaggtatgt gccgtccacc cggaaggacc
     3061 cgatggcggc gtcggcgatc ggccccacga gcgcatccga cggagtgcgc acaccgccgg
     3121 acagatatac ctgcggtgcg tcggcggcat ccgcggcggg atcggtcagc acctcgaaga
     3181 cactgatcga gttcgtcacg acggtgatcg atgaccgacg ccgcagtcgg cgagccaact
     3241 cggtggtagt ggtgcccgca ctgatcgcga tcgtcatccc gtcctcaacg gtttcggccg
     3301 cgaccgccgc gatcgcggcc ttctcggcgc gctgttgagc cgccttcacc gacgaccccg
     3361 gttcttcgcc gcggttgtgc cgggtgaccg cgccgccgtg caccttgcgc agcagattct
     3421 gcgcatcgag agcgtcgaga tcgcgacgga cggtcatctc cgacacgtcg agttcggcgg
     3481 ccaggtcggc gacccggacc gcaccaccgg cgttcaacgc ccgcaggatg gcatatcggc
     3541 gttcagcggc gagcatggga acgcacaata tcaaacaaat tttctcgatt tgggtcagtt
     3601 ggctgggtct ggactgttag tttctggttg cttctgaccg aacttgtgct gcctccgatg
     3661 aaggagttga tgaccacgac caccacgaca ctggcggcag aaaatgtgct ccgcctcaac
     3721 ctcggctggc tcgactacgt acttgtcgcg atctacttcg tcttcgtcct cggcatcggc
     3781 ttcatcgccc gaagccagat ctcgacgagc ctggacttct tcctgtccgg ccgccggctg
     3841 cccgcctggg tgaccggcct ggcattcatc tcggcgaacc tcggcgccgt cgagatcatg
     3901 ggcatgtcgg ccaacggtgc ccaggtcgga ctgtcgacca tgcactacta ctggatcggc
     3961 gccatcccgg ccatgctgtt cctcggcgtg gtgatgatgc cgttctacta cggctccaag
     4021 gtacgaagcg tgcccgagtt catgcgacgc cgcttcggcc ccggcgccca tctggtgaat
     4081 gcgatcagct tcgccaccgc ccaggtgctc atcgccggcg taaacctgtt cctgctggcg
     4141 acggtcatca atgcactact cggctggccg cgctggatct cgctgatcgt cgccgccctc
     4201 gtggtgctca gttacatcac actcggcggg ctgtcggccg cgatctacaa cgaggtgctg
     4261 cagttcttcg tgatcctggc ggcgctggtt ccgctcacgg ttttcgggct gatcaggatc
     4321 ggcggctgga atggcctgaa ggacaaggtc attgacaccg tgaccgacgg caaggtgact
     4381 gcctcggtgc acgagcaggt caccacgtgg cccggtcagg cgttgtccgg tttcgagagc
     4441 ccggtgtggt cggtgatcgg catcgtcttc gggctcgggt tcgtgctgtc cttcggatac
     4501 tggacgacca acttcgtcga ggtgcagcgt gcgatggcct cgagttcgat ctccgcggcg
     4561 cgtcgctccc cgatcatcgg ttcgttcccg aagatgttcg ttccgttcgt gatcatcgtg
     4621 cccggcatga tcgccgcggc cgcaatcggc gacatgatcc ggctgaaaac gactggcgaa
     4681 ggtgacatca cctacaacga tgcgatgttg ctgatgatcc gcgacatcct gccgaatggc
     4741 ttgctgggtg tcgctgtcgc gggtctgctg gcttcgttca tggcgggcat ggcggccaac
     4801 atctcggcgt tcaacaccgt gttcagctac gacatctggc aggactatgt ggtcaaggac
     4861 cggcccgaca actactacat cctggtaggt cgtatcgcca cgatcggggc gacgatcctc
     4921 gccattttca ccgcgttgat cgcggccggt ttctccaacc tgatggacta cctgcagacg
     4981 ctgttcggct tcttcaacgc tcccctgttc gccacgttca tcctcggcat gttctggaag
     5041 cggatgacct cgaccgcggg ctgggtcggc ctcgtctccg gaaccctttc tgccatcttc
     5101 attttcattc tgtccaaggt cggagtgttc agccttcccg ggcagggtgt gcctttcgtc
     5161 gccgcctcgg tggcgtttgt cgtcgacatc atcgtcagcg tggccgtcac catggtgacc
     5221 acgccgaagc cggcgaatga gttggtggga ctggtgtact ccgagacacc aagagaggcc
     5281 ttccacgacc ccgagagcgt aggaaaggct tggtactacc ggcccgtccc gctcgccgcg
     5341 gtttcgctcg ccatggtcat cgtcctcaac atcgtcttcc actgataagg aactgtgatg
     5401 cggcatttgt ttgacgttcg caacatcatc ggcgcactgc tgggaatcta cggtgtcgtg
     5461 ctcaccatcg ccggctttgc accaggcctg ctcggggatc accacagtgg cgccaccgac
     5521 aaccccgtcg acctgtacgt cggcaccgac gccaactggt gggtgggcct cgcgttgatc
     5581 gtcgtggcga tcgtgttcat cgggtgggca ttgctgcgcc cggtcgaggt gcccgaaaca
     5641 ccgccgcagg caacagattc ggagcaacct caggtggagt gacgcaccac cgccgcctcg
     5701 agctgctcct cgtaggcgcc ctcgtcgcgg cccagcgcga tggtggccgc cgccatcgcg
     5761 accgtgctga tcgtgatgac cacggcatcg aggtggctga cgtcgaggtt ttccccgagc
     5821 accaccgcgc cgagcagcac ggcgatgacg ggttcgagca ccagcatcgt gggcactgac
     5881 gtctgcagcg agccggcgtg gaaggcggac tgctgcagga acgtcgcgaa gacggcgatc
     5941 accgcaagca gatacaccac cggcgtggcg aatacagcgc gcacgccctc atgggtcagc
     6001 acgtgcatca ccagcttggt gagtacggcg acgacaccga acagcacacc caccgcgaag
     6061 gcgagcaaca ccgcccgctt ccatcccatg gtgcgggtgg caacgatgac gcatgcgcag
     6121 accgcggccg tgcagatgac ggcgaccaaa gtggatgtcg tcagcgaggc ctcatagtcg
     6181 ccaggccggg tcttcgccaa cacgacgaac accgctagcg ccgtcgtcag caccaccgcc
     6241 catacccatt caccgcgggt gacgcggcgg tgcgccaacc gggcgctcaa gggcaatgcg
     6301 aacagcaggg cggacaccag gatcggctgc accaacagca gcgagccctt cgcgagcgcc
     6361 aacgcctgaa agacgtagcc cgcgacggcc gcccctgtgc ctgcccacca cagcggccgg
     6421 cgaagcagag tcaagaacat caccgagctc acgccgtgtt cttgggggac gtccatggtg
     6481 gcgcgttggc gcacgacgat gccgatcgcc aggaacaccg cggcgagcac ggcgagcagt
     6541 acgaccagac catgccctac caaagcgtcg agctcctttc gggccacggc ccccacacgg
     6601 aacaagaaca acatattcgc cggcacagcg cccagcgcag gccggtctca acatggtttc
     6661 gaaccgggcc gagcgggcat agaacagcac atgaacgtgc gacggataat tttgctggtg
     6721 ggggcggtcg tgctgctggt cggcgtcatc ggactgctca tgcccgtgtc ggtgccgggc
     6781 cctgacaacg gcagcatcgg gtgcggcaat ggccttgttc aggattcatc cgccgcgcgc
     6841 gaggcggaca acaacaaccc tgtcaacctg ccgatactca acgaaatcat cccgcacacc
     6901 gactacgtca cccagtgtca atcggcggtg tcgggtcgac gagcgtggac cattccggtg
     6961 gccgtcgtgg gcctggtggt gctagccggc tcgttcttcg tcgggggccg ggccggcgtg
     7021 cggacgccgt agcggctagc tggcttcgcg gctgctgacg aggtgcagct gcagctgctt
     7081 gggttcgtcg gcaccgagcc ggtcattgac ggtcgcgacc accgagtcga cccggttcac
     7141 ggcccggtca cacagatcgc gcaccttgtc accggccgca tcgacatcgt cggcggcgct
     7201 gagcagatac gccctgacat tgacccgcaa ccgttcgccg gcacggcgca accgacggcg
     7261 cagccgatcg tcgacctcga cggtgacgta gggcaatacc tgacgtttca tctcagcgcc
     7321 tccccttcgt ggatgcctgg catcacccta ctggggtggc gctgaccgag ctatcagccg
     7381 gacccgctgg acttgtcctc ctgcgccttc tgcatggcgg ccttgccctc ggggctcgcg
     7441 gccgagttgc cgggatcctt ggcggtcgcc accgtggcat tgcaattcag gtagacgagc
     7501 accgtgtcgg tcacgggcgc gaaatcgtcg cccttgatcc atggcgccga ctgcgaatga
     7561 gtcacgatgc agttgtcgtc cgtcaatgtg tcaccggctc ggctggcgat gacggccttt
     7621 ttgccagcat cgctgatggc cttcgacgca tcgccgtagg tctggccgct gtagtcgtcg
     7681 gccgcggcga cgccgcttcc gaagaacgcc atggacacgg ccgtcgcacc gacagcggtg
     7741 atggcgtaaa cacccaattt cttcacgtgc gacctccgtg tgatgaagct gtggttgtgc
     7801 tttgcggaca ctgagtgtct tgtgcacagg agattagagt cgcgggctgg aaggacgctg
     7861 ggaagaacga tggcacttgc tgctaatccc cgtgcggcct tgacagggcg tctcgatttg
     7921 gaccgattcg tctcccccgc aatattggga gtacccgaca attgtggcgt atcgtgcagt
     7981 ttccgcgttg acgaggacgt tttctggacg atatagttca gttctcgaaa cagataggac
     8041 tcatcatgag gtcaggcctt acggctcgtc gtctcgccgc caccgccggc attggcgcga
     8101 tcatcgccat gggcgcgctc tccgcctgcg cgaaggagga gaaggcgccg gaaaccacca
     8161 cgcccactac gaccaccaca acgactacgt cggaggccac ccctgctccg ccgccgcccg
     8221 cgccgacgga gaagtcgatc aacccgaccg gtggcaacct gttcacgcct ccggtgaagg
     8281 cgccgccggc gccgaccggc gtccccggcg acaactaacc gtcggactgg tcgctgcggt
     8341 ccacttcgcg gccgcccgtc accgcaatgc tgtgccggcg gtcgcgaagg atgccacatg
     8401 gccgatggcc atgttcagcg ccgactgcat gagcctcggg tcacggcccg tctgcgacag
     8461 cagatagcct ccctccaccg ccgcaagtag ccccgtcgca agggctttcg cgtcgacgtc
     8521 ggcgacgagc gcgccgttgt cgcgcatccg ctcgaccgca gccgcgagca ggccgcgcca
     8581 ttcggtgaaa tgctcggaca gcgatgccct ggctttgtcg tccgtgtcgg acaattcggc
     8641 tgccagagaa ccgagttcgc aaccccacag cccgcgtcgc aacgcacacc gctgcaccat
     8701 cgcgtctcgc caatggtaaa ggccacggaa agaatccacc ttctccagcc gcaggcgctg
     8761 ccacgacaga atctcgtcgg cgcgcatggt gatgacctcg taaaccaact ccgatttgtc
     8821 gtcgaagtgt tggtagaact gggatttact cgacgcgctg gccgccagca cgtcgtcgat
     8881 cgtggtcgct gcgacgccct gcacgtgcat cagttcggcg gcggcaagga cgatgcggtt
     8941 gcgggttgct gcaccgcgtg atgtcaactt gggccgcgtc gcgcggtcgg tctgctgcgg
     9001 cacgccctaa ctgtaactcc gtcgctatcc ggccgtcgca gccccgcgaa catcggtgcc
     9061 cgattggaac acttcgtccg ctacaccttg cggcagcggc cgccgtgcgg tctcgcggat
     9121 agtcaggatc gtcagcagcg acacgatcgc ggccaccacg acgtagtagg cgggcgccac
     9181 gttgttgccg gtctcggcgg tcagccaggt gaccgcgtag ggcgtggtgc cgccgaacgc
     9241 ggcgacgcag acgttgtagc cgatggagaa gccgctgtag cgc
//
LOCUS       DASRJA010000653         5958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1386972,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000653 DASRJA010000000
VERSION     DASRJA010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386972"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..705)
                     /locus_tag="VFC01_29365"
                     /pseudo
     CDS             complement(1..705)
                     /locus_tag="VFC01_29365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
     gene            complement(914..1139)
                     /locus_tag="VFC01_29370"
                     /pseudo
     CDS             complement(914..1139)
                     /locus_tag="VFC01_29370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256165.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1543..1746)
                     /locus_tag="VFC01_29375"
     CDS             complement(1543..1746)
                     /locus_tag="VFC01_29375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83763.1"
                     /translation="MRRFGGYAAIRVLPDEVFDIPTWTHPKVAPDRHAQVAKALYSLP
                     GELVGRRAGCPRRCAHSETVLAW"
     gene            complement(1776..2036)
                     /locus_tag="VFC01_29380"
     CDS             complement(1776..2036)
                     /locus_tag="VFC01_29380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83764.1"
                     /translation="MNPMNFHRLRGNWKALSAALGVGVLITMGAATLAYPVSAVGTSS
                     DSWKADTTITLTPTPAPPSFVPKVKTSQCDFESPHALVYGCS"
     gene            complement(2372..2635)
                     /locus_tag="VFC01_29385"
     CDS             complement(2372..2635)
                     /locus_tag="VFC01_29385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83765.1"
                     /translation="MGPMNFHRLRGNWKALSAALGVGVFVTMGAVTLAYPTMEGGASS
                     DSWNAEANLTLVPATRVQPSFAPKLIADPCTHAAPHARAHGVC"
     gene            complement(3758..3991)
                     /locus_tag="VFC01_29390"
     CDS             complement(3758..3991)
                     /locus_tag="VFC01_29390"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN83766.1"
                     /translation="MGTARNCRADLLVIGAGMAGLTAAARAARDGANVVLVERAQALG
                     GSAVFAQYVWTTPTFEGLQALDPGGDPELGRAP"
     gene            complement(3991..4482)
                     /locus_tag="VFC01_29395"
     CDS             complement(3991..4482)
                     /locus_tag="VFC01_29395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83767.1"
                     /translation="MINLAVPRVRVVPDSSPAHAPLDELRCGRPVFVLDAVGRDDEGD
                     LVFAGVLATPKVPLCVVRHRSGFVRGRVRADAAGPDDGPGRRRADSADGVWKIAHRIV
                     TDDWSMWADCTEKVARLGTHTGLASKDNPSYTAWGQGFIARSNERPSMKRPIVETLRE
                     TVA"
     gene            complement(4479..4999)
                     /locus_tag="VFC01_29400"
                     /pseudo
     CDS             complement(4479..4999)
                     /locus_tag="VFC01_29400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006865949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            complement(5064..5249)
                     /locus_tag="VFC01_29405"
     CDS             complement(5064..5249)
                     /locus_tag="VFC01_29405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83768.1"
                     /translation="MSTRCNQQLTFDIPPYSAGAAKCARPLSANGTQMIVAITENDPE
                     RQLRPVRELAGSRVAAR"
     gene            5446..>5958
                     /locus_tag="VFC01_29410"
     CDS             5446..>5958
                     /locus_tag="VFC01_29410"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN83769.1"
                     /translation="MGIVGVTAQPGDGVRASTRTPVMARVPLFERLGAAARGGVTLVC
                     APAGSGKTMLLRSWIDHANLGDHTGWVSVEHDERDAQRFWRSVIDELRGTVGADAFMD
                     ELTPTPFFDGEAVVDRLVAELGSLKEPVVLLIDDLHELRSTEALAQLKMLLSRRPPLL
                     SVVLASRHDPR"
BASE COUNT         1055 a         1881 c         1987 g         1035 t
ORIGIN      
        1 aaccggacgc gccgacgatc atgccggcga cgatcatcgc ggtgttgttc agcgccaatc
       61 ccgctgccgc ggctgagagt ccggtcagcg cgttgagcag cgagatgacc accggcatgt
      121 cggcaccgcc gatcggcagc accaccatca gccccagtac accggcggcg gccagcagcc
      181 cgaccatccg ccacagccac gccccgccgc cgggatgggc ggcggggccg atgacgacgg
      241 ccgccaccac cgcgccggcg agcagcacca ggttcaacag ctgctgcgcc ttgccgatgc
      301 cgattgggcg gccgggcagg atttcctgca gcttgccgaa cgcgatcagc gagccccaga
      361 acgacaccga accgatgatt gcggcgagca acgaagcgac cacgatgtgc acgcggtcgg
      421 cgcctcgcca tgcttgaacg ccgaaaaacc ctcggtgtca atgaattccg ctagcgcgat
      481 cagtgcgacg gtgccgcctc cgacgccgtt gaacagcgcg tggcggtcat cttcgtcagc
      541 cgggcagggg cacgcccagc acaccacacc gaccagcagg ccggcgatga tcagcgccca
      601 gttctcggtg tgccggatcg cgatcagcgt ggcgatcgcc gcgatcgcca taccgccggc
      661 ggcgacccag ttgccccgcg cggcggtctt gggcccggtc aggccgccag cgaacccacc
      721 ggtggtccgt cgctgcacgt ccaggcaatc gttggcatcg ttcctcctct cgcgggagcg
      781 ccctgctccc acgacagaag gtcgcgagcg gtgccgctat tacaccgacc ctgagtcccc
      841 gcctcgcgcc aaacatgcag cgtcccaaac aacacctgca tccgcccgtt cacacttcgg
      901 aataattgcg gggtagggat aaatcgatga tgaacagctc gacgcggtgc agtttgcgca
      961 tctcgtcttc gtaggtggcg tccagccgct gctgcgcgta ggcgtttgaa gagtttgtcg
     1021 gcgcgttcgg tgtggacgct gtgatgacgc cgaacggcga tgtgccctaa ggcgttggcc
     1081 agtaacgttt ttccgacgcc gaccgggccc atgatgagca cgttgtaagc gtcggcgagg
     1141 ccttcggacg gcgaaatcgg tgctggcgcg gacgagccat gacgccgctg gcggtgccgg
     1201 caggaacggt aactgctcgc cgtcggtggg gagcacatgt ggtcgatgaa cccgacgtcg
     1261 atggcttcgg tgtacagcgc gcgccgacaa gcgtcgtcga ccgcatcggc gccgtggcgg
     1321 cgtaccagcc cgagtaggcg gtagacctgg cgcttcttgg tccacggcag cggatgctcc
     1381 aacaccgccg cggcgtacgc gccgacatga tcaccatgtg cggatgcctt gcgctgcagg
     1441 gtgttcagat cccgcatcgc atcggccaac acctcggcgg acaggtcggc gggatcggtg
     1501 tgccggcacc cgggtgccat gaccgggtgg accttgatca gttcaccacg ccaatacagt
     1561 ttcactgtgt gcgcatcgac gcgggcatcc agcccggcgt ccgaccagct cgccgggaag
     1621 gctgtagagc gccttggcca cctgcgcatg ccgatccggg gccaccttgg gatgagtcca
     1681 ggtcgggatg tcgaacacct catcgggcag cacccgaatc gccgcataac cgccgaatcg
     1741 cctcataacc accgacgatc tagcgggcga gtccgctagg agcagccgta gacgagagcg
     1801 tgcggcgatt cgaagtcgca ttgcgacgtc ttgaccttcg ggacgaagga gggcggcgca
     1861 ggtgtcggcg tcagcgtgat agtggtgtct gctttccagc tatctgagct agtgccgacc
     1921 gcactgaccg ggtaggcaag agttgccgcg cccatcgtga tgaggacgcc gacgccgagc
     1981 gctgccgaaa gcgccttcca gttgccgcgg agacggtgga agttcatggg attcatcgat
     2041 tctctccttg attgagtgtt cgcggtatgc ggaggttgtc attgccgtag cacgtgacac
     2101 caggcgtccc gggtggcgag ccgatcgtca ctgacgactc actggacaaa ctgttcagtt
     2161 gattcagttg tctttttcct gggcgcgatc gggctagccc cgcggcctgt cgggttgacc
     2221 gcagagcagg tcgactctgt cggatatcac tggctatcga gaagcaagag gtgatcagca
     2281 cgaactgtcg taatgtcatt cgcgctcagc tgacggctcc agtggcgatc gcgcggtccg
     2341 aaaattcgcc attccatagg gggaagccca cctagcagac gccgtgagcg cgagcgtgcg
     2401 gtgcggcgtg ggtgcatggg tcggcgatga gcttcggggc gaaggagggc tgcacacgtg
     2461 tcgccggaac cagcgtgaga ttagcttctg cattccagct atctgagcta gcgccgccct
     2521 ccatggtcgg gtaggcgaga gttaccgcgc ccatcgtgac gaagacgccg acgcccagcg
     2581 ctgccgaaag cgccttccag ttgccacgaa gacggtggaa gttcatggga cccatcgatt
     2641 ctctccttca tcgagtgttc gcggtatgcg aggtttttgg cgactgcaat cacctcgcat
     2701 gcggtggtgg atgtcacaat gaacggtgag gatgatgtaa gtcactacct gtccgagtat
     2761 ttcggaagct gcaggggtgg cgtaggccca ggcgcggtca gcggtgtcct gactcacatc
     2821 cgaaagggct actcgctacc ggttagtggg gtggcgtgta gcggaagcgc cgcggcgatt
     2881 cccgccccct cgtttggctt ttgtcccgcg gaggcggttt gcaacttgct gcctgcggtg
     2941 ttggggagcg agcttgggga agagaactgg gtgaagtcgg tggcacgagc gcaacatcgt
     3001 gaaaaggctg gcggctgaac gtcgttgacc tgcagctagg tgaccgccca ccctgggcag
     3061 accgcgacgg cttactgcgc tgggcatcgc gcaaggcggt gtcgaaaatc gaagtacgtg
     3121 gctggcgttc gccggcaaga gacgaattgg aggtgatccg gatcgttagg ccgccgacag
     3181 cggcacatca agcattatgg acgctcagca gcttgtgccc cgagaatcgt tgccggccac
     3241 gcaaaccgtg agcgcgggaa tcggcagaca agccccgcgg cgtgcagcag aaccacctta
     3301 acggcaattg agcgacgcga agaggcctgc gaggccatca gccacgacgc cgagagcggt
     3361 gtccgccttc catacaccgc tgtattgcgg cggcgccgtg ccgtgtgctc agcccggacg
     3421 tccgcccgag ttgcggcagc ctgcaacaag ccgatcttca ggtgcaacgg gcacgtggtc
     3481 actgtgggct gttctctggc cggaactgcc tctgaccctg cgaccgttgg gaactcaaga
     3541 tgcggctgag cagtagacgg tgtacggcag aggcgagcga tcgtgtcccg ccgcttatga
     3601 tcgattagca gcgcgccgac tcagcgaacg ccgacgtgag ggcggcatca tgtggcagcg
     3661 tcgatgtatc gacgagcgat gcacgggcga cgacaggcaa gatcagagtg atctgcggga
     3721 cggatccgcg tgattgcgca aacgtccgga gtcgccgtca gggggcacgg cccaactccg
     3781 gatccccgcc tggatccagc gcctgcaggc cctcgaatgt tggcgttgtc cagacatatt
     3841 gagcgaatac cgcggatccg cccagggctt gagcgcgttc aacgagaact acgttggcgc
     3901 catcgcgcgc cgcccgcgcc gcagcagtga ggcccgccat acctgcaccg atgaccagca
     3961 ggtcggctct acagtttctc gctgtaccca tcacgccacg gtctctcgca aggtctcgac
     4021 tattggtcgc ttcatcgatg gcctctcgtt gcttcttgcg ataaagcctt gaccccaggc
     4081 ggtgtaggag gggttatcct tcgacgccag acccgtgtgg gtgccgaggc gagccacctt
     4141 ctccgtacag tcggcccaca tgctccagtc gtcggtcact atccggtgcg caatcttcca
     4201 aacaccgtcc gcgctgtctg cccgtcgccg tcccggaccg tcatcgggtc ccgccgcatc
     4261 cgcgcgcacg cggccgcgga cgaaccctga cctgtgccgg acgacacaca acggcacctt
     4321 cggcgtggcg agcactcccg cgaagaccaa gtcgccctcg tcgtccctgc ctacggcgtc
     4381 caggacgaaa accggacggc cgcagcgcag ctcatcgagc ggcgcgtgcg ccggcgaaga
     4441 gtccggtacg acgcgcacac gtgggacggc gagattgatc atgatgcgtc cgcgaggcgg
     4501 cggaatcgac tccggtggaa aacgagaggg gcggtggcgt tcgacagacc ggtctgctgg
     4561 atgcgcagca caacgatcag gtggtcgccg acggtgaact cggactccat cgagcagtcg
     4621 aaccaggcac acgactcgcg cagatggatt gagcccgacg acaagacaac tacgtcgact
     4681 ccgtcgaacc gctcgccgtc cttgcgggca agctgtcggc aggcgcgctc gtggtgctcg
     4741 cctaggacgc tcaccccgag gttcggcgct tctcgcagac gcggccaggt gctcgaagtc
     4801 ctctggatgc acaccgagat cagtggcggg tggagcgaga ccggggtgaa gctgctgacc
     4861 gccatcccac gggatgccgg tcgagttggg cgcacaccgc caggacaccg ctcgggaatg
     4921 cgccgaacac ggtgcgcagt tcggtcgggg ctcccgcggt ggcgtcgctg ccagctcgaa
     4981 cgggatcgtt ggtgctcatc gcggcgaccg gtagccccac gagcagcctg cacgtctctt
     5041 cagcatgtgg acgctgggcg agatcaccga gcggcgaccc ggctccccgc cagctcgcgc
     5101 accggcctga gctggcgctc ggggtcgttc tcggtaatcg cgacgatcat ctgggtgccg
     5161 ttagccgaga gcggccgcgc gcactttgct gcccctgccg agtagggcgg aatgtcgaag
     5221 gtcagctgtt gattgcacct tgtcgacacc ggcgctcccc tccgcgggtc agcagacaaa
     5281 ccagctgaca tatcaggacg ctgatgatat atcatgcatg agtgttggtt ttaggtcaag
     5341 cactgccaga ggagttccca tgcccgctgg cgcgctcctc gagggatcat ccttccgctg
     5401 aactgcgtcg atcaagagga cctgtgagct agtcggggtg gaggcgtggg catcgtgggc
     5461 gtcaccgcac aacccggcga tggcgtgagg gccagcaccc ggacacccgt gatggcgcgc
     5521 gtaccgttgt tcgagcgcct gggggccgcc gctcggggcg gtgtaacgct ggtgtgcgcg
     5581 ccggccggca gcggcaagac gatgttgctg cggtcctgga ttgaccacgc caacctgggt
     5641 gatcacaccg gatgggtgtc ggtcgaacac gacgagcgcg acgctcagcg cttttggcgg
     5701 tcggtgatcg atgagctgcg gggcacggtt ggcgcggatg ccttcatgga tgagttgaca
     5761 cccaccccct tcttcgacgg tgaggcggtg gtcgaccgtc tggtggccga gctgggctcg
     5821 cttaaggagc cggtcgttct gctgatcgac gatctgcacg agcttcgctc caccgaggcg
     5881 ctggcacagc tgaagatgct gctttcgcgg cgaccgccgc tgcttagcgt ggttttggcg
     5941 agccgccacg acccgcgg
//
LOCUS       DASRJA010000654        16299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3187817,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000654 DASRJA010000000
VERSION     DASRJA010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16299
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3187817"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFC01_29415"
     CDS             <1..396
                     /locus_tag="VFC01_29415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HZN83770.1"
                     /translation="DVGRTASPHVWAIGDVASWRHPVGHQVRVEHWSNVADQARAMVP
                     AMLGKDVPATVTVPYFWSDQYDVKIQCLGEPEATDTVHVVEDDGRKFLAFYERDGVVA
                     GVVGGGMPGKVMKVRNKIAAGAPIADVLG"
     gene            complement(401..1687)
                     /locus_tag="VFC01_29420"
     CDS             complement(401..1687)
                     /locus_tag="VFC01_29420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN83771.1"
                     /translation="MNNSHDPLSVLHGDELPVQPDPGFAARLRTRLESAVSLPNRTQG
                     VVMTGTDTAIAELNEPAASVASAPPRPAALPYLAVANAREAIAWYVDTFGASVVGAPF
                     EMDDGRIGHAELAIGGGVLYLADEYPELGLKAPDPQATSVSLMLHVADTDATLERARD
                     RGAHVQLEPYENYGARNATIVDPFGHRWMLSGPATGAATPIQHGDVGYVSVWTPDAER
                     AAAFYGHILGWTYDPSTHQVTNTTQHIGIFSVDSRQGMLCCYAVADLQGARQSILDGG
                     GAVGEPQQFDFGTVLDATDPAGMVFAVFQPAAGEPRPALNGSGPGELSYITYEVTDST
                     AFKAFYSRVLFWTFEPGRIDDGWGVQQTHPMAGVAGGSTAHVTVPMWTVEDVDAAVAR
                     VREAGGRVIEEPSQQAYGKMAQCTDDQGTRFYLGEF"
     gene            complement(1684..2208)
                     /locus_tag="VFC01_29425"
     CDS             complement(1684..2208)
                     /locus_tag="VFC01_29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN83772.1"
                     /translation="MSAEPDGNEGPRALLALYDDALPVVYGYFVRRCADRGTAEDLTS
                     ETFLAAMDAARKDSPPPMTVPWLLGVARHKLADHYRRRHDRFSIPVAELPEPVDPADN
                     WDAALDRIVAEGVLARLPEQHRTVLALRYMDDCSVPECAELIGRTVHATEALLVRARR
                     AFRSHYPEREGGKS"
     gene            complement(2248..2874)
                     /gene="mftR"
                     /locus_tag="VFC01_29430"
     CDS             complement(2248..2874)
                     /gene="mftR"
                     /locus_tag="VFC01_29430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245092.1"
                     /note="MftR, the mycofactocin system transcriptional
                     regulator, is an uncharacterized TetR family DNA-binding
                     transcription factor. Its role is inferred by context. It
                     occurs as part of the biosynthesis locus for mycofactocin,
                     a partially characterized electron carrier derived from
                     the terminal Val-Tyr dipeptide of the precursor peptide
                     MftA, through a radical SAM enzyme-mediated process;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0036042 - long-chain fatty acyl-CoA
                     binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system transcriptional regulator"
                     /protein_id="HZN83773.1"
                     /translation="MRPVSQHRVGRRRSTTWDHISNVAIDLFAAHGFDEVSVDDVAEA
                     SGIARRTLFRYYASKNALPWGDFDAHLDHMRDLLNHSEPDVPIRAALRAALLAFNTFD
                     ETETARHRQRMRVILETAALQAYSMTMYAGWRAVVAEFVAKRLGTKAEDLVPQTVAWT
                     MLGAALSAYEHWLADESVPLMQALGDAFDTVSGGLSALDTKASRKNSQ"
     gene            2965..3090
                     /gene="mftA"
                     /locus_tag="VFC01_29435"
     CDS             2965..3090
                     /gene="mftA"
                     /locus_tag="VFC01_29435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305005.1"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes
                     in Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin precursor MftA"
                     /protein_id="HZN83774.1"
                     /translation="MEPNQEVGTEAGAATAATSGEALVTETLVEEVSIDGMCGVY"
     gene            3075..3380
                     /gene="mftB"
                     /locus_tag="VFC01_29440"
     CDS             3075..3380
                     /gene="mftB"
                     /locus_tag="VFC01_29440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305006.1"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ,
                     a cofactor that derives entirely from a Tyr and a Glu in
                     the precursor PqqA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="HZN83775.1"
                     /translation="MRGLLIVSAPAAAFDPDLRWRLHHQVAVRPEPFGALLYHFGTRK
                     LTFLKNRTIVEVVNSLSDHPDARSACRAAGIDDAQQAPYLHALGVLAQSKMLVTGEA"
     gene            3377..4546
                     /gene="mftC"
                     /locus_tag="VFC01_29445"
     CDS             3377..4546
                     /gene="mftC"
                     /locus_tag="VFC01_29445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215207.1"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron
                     carrier mycofactocin from the terminal Val-Tyr dipeptide
                     of the precursor peptide MftA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="HZN83776.1"
                     /translation="MTSVAPVPRLVEQFERGLDAPICLTWELTYACNLACVHCLSSSG
                     KRDPRELTTQQCKDIIDELERMQVFYVNIGGGEPTVRPDFWELVDYATAHHVGVKFST
                     NGVRITREVAERLAASDYVDVQISLDGATAEVNDAVRGQGSFAMAIRALENLAEAGFS
                     DAKISVVVTRHNVDQLDEFGALAARYGAMLRITRLRPSGRGADVWDELHPTAEQQVQL
                     YDWLVAKGDRVLTGDSFFHLSGLGEPGALAGLNLCGAGRVVCLIDPVGDVYACPFAIH
                     DRFLAGNILSDSGFDNVWKNAPLFRELREPQSAGACSSCGHYDSCRGGCMAAKFFTGL
                     PLDGPDPECVEGYGAPALARERVKPKPSVDHSRRGPVMLKLLTKPPVRLCNESPV"
     gene            4550..5743
                     /gene="mftD"
                     /locus_tag="VFC01_29450"
     CDS             4550..5743
                     /gene="mftD"
                     /locus_tag="VFC01_29450"
                     /EC_number="1.4.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167825.1"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the
                     non-exchangeable NADH of TIGR03971 family SDR-type
                     oxidoreductases; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="HZN83777.1"
                     /translation="MARDQWFETVAFCQQAAKKRLPKSVYSALLGASERGVTVADNVD
                     AFAELGFAPHVIGALEKRDLSTTVMGQDISLPVMISPTGVQAVHPDGEVAVARAAAAR
                     GTAMGLSSFASKPIEEVIAANPKLFFQVYWLGGRDAIAARVERAREAGAVGLIVTTDW
                     SFSHGRDWGSPKIPEEMNLRTTVRMLPEGLTRPRWFLDWAKTMRPPDLRVPNQAPRGE
                     PGPPFFAAYGEWMATPPPTWEDIAWLRELWGGPFMLKGVMRVDDAKHAVDAGVSAISV
                     SNHGGNNLDGTPASIRALPAIADAVGDQIEVLLDGGIRRGSDVVKALALGARAVMIGR
                     AYLWGLAANGQAGVENVLDVLRGGIDSALMGLGRASVHDLVAGDVLVPPDFSRALGVP
                     PASNA"
     gene            5877..6620
                     /gene="mftE"
                     /locus_tag="VFC01_29455"
     CDS             5877..6620
                     /gene="mftE"
                     /locus_tag="VFC01_29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009937382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="HZN83778.1"
                     /translation="MNSAYHRRVAFPSELANLTSRQLQTTSPALIIPVGSVEQHGPHL
                     PLDTDTRIATAVARLLADRLADANESNWALAPAIGYGASGEHEGFPGTVSIGTSALRL
                     LLVEFGRSASQWASRLVFVNGHGGNVEALAAAVALLRYEGRDVAWCSCTAANADAHAG
                     HTETSVLLHISPADVWVDERLPGNREPLAQLMPQMRQGGVAAVSELGVLGDPTTATAE
                     EGERILAEMVDACLLRITRWAPDRDGMLT"
     gene            6617..8029
                     /gene="mftF"
                     /locus_tag="VFC01_29460"
     CDS             6617..8029
                     /gene="mftF"
                     /locus_tag="VFC01_29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209156.1"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="HZN83779.1"
                     /translation="MTGPRLPDGFAVQVDRRVKVLGEGSALLGGSPTRLLRLAPAAQT
                     MLTGGRLEVHDAVSAQLARTLLDATVAHPRPATGPSHRDVTVVIPVRDNTSGLLRLVG
                     TLRGMRVVVVDDGSATPVQQSDFADMHCDIQVLRHTRSKGPAAARNTGLVACTTDFVA
                     FLDSDVVPRRGWLEALLGHFCDPAVALVAPRIVGLRQADNLVARYEAVRSSLDLGLRE
                     APVVPFGTVSYVPSAAIICRRAVIKDLGGFDETLKTGEDVDLCWRFIEAGARLRYEPI
                     ALVAHDHRTQMREWFVRKAFYGESAAPLSIRHPGKTAPLVISGWTLVVWILLAMGSGI
                     GYLASMVVAAVTGRRIAKSLSTVETEPKEVAVVAAHGLWSAALQLASAICRHYWPIAL
                     IAAVLSQRCRHVVLVAAVVDGVVDWITRNGNADDDTKRVGLLSYVLLKRLDDIAYGAG
                     LWTGVVRERHAGALKPQIRT"
     gene            8037..9464
                     /gene="mftG"
                     /locus_tag="VFC01_29465"
     CDS             8037..9464
                     /gene="mftG"
                     /locus_tag="VFC01_29465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system GMC family oxidoreductase
                     MftG"
                     /protein_id="HZN83780.1"
                     /translation="MHSDVLIVGAGSAGSVLAERLSADPQCRVTVVEAGPGPSDPRVL
                     SQINGLHLPIGDASSVVRRYPTTLTDDPKRLAQIMRGAVVGGSGAVNGGYFCRGLPMD
                     FDSWGLPGWSWADVLPHFRAIETDLDFDNPLHGSDGPILVRRVDEFDGCTASFVRSAG
                     EAGYRWIDDLNGATPEEPLPPGVGAVPLNINGGTRVGPGGAYLQPAMDRTNLTLLTNT
                     RVRRVRLTGGRAVGVECVGPDGSGDLTADRIVLSAGAIGSAQLLMLSGLGPREVLRAA
                     EVPVVADLPVGVACVDHPEWVLPVDWSETHGLPPLEAVLTTDTLEIRPYTAGFGAMVS
                     GRRDDPGDHPHIGVALMQPESRGRVAVTSADPDTPPLIEHRYDHAPADVAQLDAGAEL
                     ARELVGATAQLGRVSWSTSQHLSSTAPMGLDGDAHAVVDQQCRVRGVENLWVVDGSVL
                     PGITSRGPHATIVMIGHRAAEFIVG"
     gene            complement(9360..11030)
                     /locus_tag="VFC01_29470"
     CDS             complement(9360..11030)
                     /locus_tag="VFC01_29470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN83781.1"
                     /translation="MVDTLARSDQDIDASAATLSKRARFWLLTVAALDVLLVISSMVA
                     LNAALPDIALQTSASQTQLTWVVDGYTLVLACLLLPAGALGDRYGRRGALLVGLAIFA
                     VASLSPTIFDSPVQIIVARAVAGIGAAFIMPATLSLLTAAFPKSERNKAVGIWAGVAS
                     SGAIVGFMGTGLLLKFFSWQSIFYGFAAAALGLLLCTCTIASSRDETATPLDWLGAVL
                     IGGAVAVFVFGVVEAPVRGWTHPIVWGCMLAGVALAGAFAAVELRRKHPLLDVRLFGR
                     PDFATGSIGITLLFFANFGFFFVEMQYMQLILGYSPLQTAFALTPLAVPIMVLGATMH
                     LYLPRIGLRVAVSLGLFLIAAGLFSMRFLDAGSTYLDLVWPLLITSAGIGLCVAPTTS
                     AIMNAVPTEKQGVASAVNDTTREVGAAVGIAVAGSVLAAQYHNLLSPGVAEFPQHVRE
                     SALDSLAHALAVAAQMGPQGARLAELAESAFIDSMDLSLLVVSIVLAVAAAFVAVWAP
                     GRDGRQFGFLRRRPLADDELGRPVPNHHDRGVRPAAGDPRQDRPVDDP"
     gene            11121..11789
                     /locus_tag="VFC01_29475"
     CDS             11121..11789
                     /locus_tag="VFC01_29475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN83782.1"
                     /translation="MTAIGNDPRPARSRARLLDAATALLRSGGPSAVTIDAVTRSANV
                     ARATLYRHFSSANDLLAAAFTGLLQPAPMPPAEGSLRDQLIAVVVGWAESIAEVPTTI
                     TAMTWLASGPDAGAYSEVRQTDSDAVGTLRARIIQMYSAPFDAIFDSPQAAAELGDVD
                     RVQAIALLIGPLILGRLSTLADFDYRTCAGAAVDGFLATHAKARNVTSASSGSAGASG
                     RRQK"
     gene            complement(11737..12987)
                     /locus_tag="VFC01_29480"
     CDS             complement(11737..12987)
                     /locus_tag="VFC01_29480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN83783.1"
                     /translation="MSTPTMDDAAKVLADPTAYADDARLHAALTHLRANNPVAWVDNP
                     PYRPFWAVTKHADIMAIERDNTLWINEPRPLLATADADDMAQEMLQAGGGLRTLIHMD
                     DPQHRVIRAIGADWFRPKAMRDLKIRVDELAKIHVDKMVAAGGECDFVHEVAVHFPLY
                     VILSLLGLPESDFPRMLKLTQELFGGDEEEYKRGTTLEDQNAVLLDFFGYFTSLTASR
                     REHPTEDLASAIANARIDGQPLSDLETMSYYVIIATAGHDTTSATIAGGLRALIDNPG
                     ELQRLQQNPQLLGTATEEMIRWVTPVKEFMRTATADTTVRDVPIAEGESVYLAYVSGN
                     RDEEVFDDPFRFDIGRDPNKHVAFGYGVHFCLGAALARMEINSFFSELVPRLESIELA
                     GEPELASTTFVGGLKHLPIRYSLR"
     gene            13104..14696
                     /locus_tag="VFC01_29485"
     CDS             13104..14696
                     /locus_tag="VFC01_29485"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HZN83784.1"
                     /translation="MPARSLHRLLALAFVLLLAVGCSRGGGESPKTAAPTHQSDPAVV
                     QTADGTLRGIVAPDHRLFAGIPYAAPPIGPLRWQPPDPAAPWQGVRDATRVGPRCLQD
                     PGGDLELGRQTDEDCLTLNVWTPPVDPSRGQRPVMVWIHGGSFVNGSGGIYDSRWLAA
                     RGDMVVVTVNYRLGATGFLAHPALGPAGDVGNYGLADQQAALRWVRDNIADFGGDPAK
                     VTLAGESAGGISVCDHLVAPDSAGLFRAAIIQSAPCQAQLALPAAEKSSLDYAATVGC
                     ADPQSAAQCLRALPAGKLRDPVWYFKIGDDALSGPVTDTRSLPVDPMAAFADGRSARV
                     PVLIGTNHDEFTLFVALQYLRLGREYTTDQYPQLLRETFGDYAAAVEEHYPLQRYSSV
                     PLAYSAAVTDGVFACVSDRMAKAMARTQPVYAYEFNDRNAPTSEPMRTLPFPVGASHS
                     LELRYLFDIGDAQPLNAAQQVLSDQMIDYWAHFVHTADPGSQWPAFGPDEKRLSFQSD
                     GSRVTTDYDKTHQCAFWASLKG"
     gene            14702..15055
                     /locus_tag="VFC01_29490"
     CDS             14702..15055
                     /locus_tag="VFC01_29490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN83785.1"
                     /translation="MPTPDPKSYADQWVRAWNGHDIEAVLDHFHDDVVFTSPVAVRVV
                     PESGGVVHGKPALRDYWTAALAKQPDLQFDVVGVYRGESTLVINYRNHRGELVNEVLT
                     FDGDKVREGHGTYLA"
     gene            complement(15052..15360)
                     /locus_tag="VFC01_29495"
                     /pseudo
     CDS             complement(15052..15360)
                     /locus_tag="VFC01_29495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055627.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
     gene            15379..15528
                     /locus_tag="VFC01_29500"
     CDS             15379..15528
                     /locus_tag="VFC01_29500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83786.1"
                     /translation="MRTAAYTKISQDAAGERAGVARQLEDCLAFADRLGWDGGCGGSG
                     PNVLC"
     gene            complement(15797..16270)
                     /locus_tag="VFC01_29505"
     CDS             complement(15797..16270)
                     /locus_tag="VFC01_29505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83787.1"
                     /translation="MKVHKVIAGSVLAAGLGVAGMFGAGTASALGFAVDPGAGDGFSV
                     GTGATADVSEGNTGVAISVFGTASATTQGNASGNNLLAIDGSSTASGNATGNNVVSVL
                     GASSVSGNAHDNTLVNVGGVVTASGNASSATSLSACGTSFTGQAAHVTVSPGICG"
BASE COUNT         2754 a         5243 c         5498 g         2804 t
ORIGIN      
        1 gacgtcggtc gcacggcgtc gccgcacgtg tgggcgatcg gcgatgtggc ctcgtggcgt
       61 cacccggtgg gacaccaagt gcgcgttgag cattggagca acgtcgccga ccaggcccgc
      121 gcgatggtgc cggcgatgct cggcaaggat gtcccggcca ccgttacggt gccgtacttc
      181 tggagcgacc agtacgacgt caagatccaa tgcctcggtg aaccggaagc caccgacacc
      241 gtgcatgtcg tcgaggacga cggccgcaag ttcctcgcgt tctacgagcg cgacggcgtg
      301 gtcgccgggg tggtcggcgg cggcatgccg ggcaaggtga tgaaggtccg caacaagatc
      361 gccgcgggcg ccccgatcgc cgacgtcctc ggctgagcga tcagaactcg ccgaggtaga
      421 agcgggtgcc ctggtcgtcg gtgcactgcg ccatcttccc gtaggcctgc tgcgacggct
      481 cctcgatgac cctgccgccg gcctcgcgca cccgggctac cgcggcgtcg acgtcttcga
      541 ctgtccacat cggcacggtc acatgcgcgg tgctgccgcc cgcgacacct gccatcggat
      601 gggtctgctg aacgccccag ccgtcgtcga tgcgcccggg ctcgaacgtc cagaacagca
      661 cgcggctgta gaacgccttg aaggcggtcg agtcggtgac ctcgtaggtg atgtacgaaa
      721 gttcgcctgg cccactgccg ttgagggccg ggcgtggctc gcctgctgct ggctggaaga
      781 ccgcgaacac catacctgca gggtcggtcg cgtccagcac ggtgccgaag tcgaactgct
      841 gtggttcgcc gactgcgccg ccgccatcaa ggatcgactg ccgggcgccc tgcaggtcgg
      901 cgacggcgta gcagcagagc atgccctggc gactgtcgac gctgaagatc ccgatgtgtt
      961 gggtggtgtt ggtgacctga tgggtcgacg ggtcgtaggt ccacccgagg atatggccat
     1021 agaacgcagc ggcgcgctcg gcgtcgggcg tccacaccga gacgtagccg acgtcgccat
     1081 gttggatcgg cgtcgcggct ccagtcgccg gtccgctgag catccagcga tgcccgaacg
     1141 ggtcgacgat cgtggcattg cgcgccccgt agttctcgta cggctccagc tgtacgtgcg
     1201 caccgcggtc gcgggcacgt tcgagcgtcg cgtcggtatc ggcgacgtgc agcatcaggc
     1261 tcaccgaagt cgcctgcggg tcaggcgctt tcagtccgag ctccgggtac tcatcagcga
     1321 ggtagagcac gccgcccccg atcgccagct cggcgtgacc gatacggccg tcgtccatct
     1381 cgaacggtgc cccgaccacc gaagcgccga aggtgtcgac gtaccaggca atggcctcgc
     1441 gcgcgttcgc cacggccagg tatggcagcg cggcggggcg cgggggagcc gacgcaaccg
     1501 aagcggcagg ctcgttgagt tcggcgatgg cggtatcggt gccagtcatg acaactcctt
     1561 gcgttcgatt gggaagagac actgcggatt ccaggcgcgt gcgcaggcgg gccgcgaacc
     1621 ccggatcggg ctggaccgga agctcgtcgc catgcagcac ggacagcgga tcgtggctgt
     1681 tgttcatgac ttccctcctt cccgctccgg gtagtgagat ctgaaggcgc ggcgggcgcg
     1741 caccagcagc gcctcggtgg cgtgcacggt gcgcccgatc agctccgcgc actcgggcac
     1801 cgagcagtca tccatgtagc gcagcgccaa gaccgtgcgg tgctgttcgg gcagtctggc
     1861 aagcacgccc tcggcgacga tgcggtccag cgcggcgtcc caattgtccg ccgggtcgac
     1921 cggctcgggc agttcggcca ctgggatgct gaaccggtca tgccgacgcc ggtagtggtc
     1981 ggctagcttg tggcgcgcca ccccgagcag ccacggcacg gtcatcggcg gcggtgagtc
     2041 cttgcgagcg gcgtccatcg ccgccagaaa ggtttccgac gtcaggtcct cggccgttcc
     2101 gcggtcggcg cagcgcctga cgaaataccc gtacacaacc ggcagcgcat cgtcgtacag
     2161 cgccagcagg gcccgcggac cctcgttgcc atccggttcg gcgctcacac ccttatcgtc
     2221 gccgtcggag gccaaactcc gacgccccta ttgcgaattt tttcgcgagg ccttggtgtc
     2281 cagcgccgac agcccgccac tgaccgtgtc gaacgcatcg cccaacgcct gcatgagcgg
     2341 caccgactca tcggcgagcc aatgctcgta ggccgacaac gccgccccga gcatcgtcca
     2401 cgccacggtc tgcggcacca gatcttcggc cttggtgccc agccgctttg cgacgaattc
     2461 ggcgacgacc gctcgccagc ccgcgtacat cgtcatcgaa tacgcttgca gcgcagcggt
     2521 ctccaggatg acccgcatcc gctgccggtg ccgggcggtt tccgtttcgt caaaggtatt
     2581 gaacgccaac agcgctgcgc gcagcgcggc ccgaatcggg acgtcgggtt ccgaatggtt
     2641 gagtaggtcg cgcatgtggt ccaggtgcgc gtcgaagtcg ccccacggca gcgcgttctt
     2701 ggaggcgtaa tagcgaaaca gggtgcggcg tgcgatgccc gatgcctcgg cgacgtcgtc
     2761 gacgctgacc tcgtcgaacc cgtgagcggc gaacaggtcg atggccacgt tggaaatgtg
     2821 gtcccacgtc gtggagcgcc gtcggcccac ccggtgctgg gaaaccggcc gcatagaccc
     2881 cacccttcca tttcggcact cgatgccata ttattgcgac ctcggtcaca ttgtcgagac
     2941 ccaacgcgac gaaaggtgct gttcatggag cctaaccaag aggtagggac cgaggccgga
     3001 gccgcaacgg cggcgacatc aggcgaagca ctcgtcaccg agaccctggt cgaagaggtg
     3061 tcgatcgacg ggatgtgcgg ggtctactga tcgtgtcggc gcctgcggcg gcgttcgatc
     3121 cggacctgcg ctggcggctg catcaccagg tggcggtgcg gcccgagccg ttcggtgcgc
     3181 tgctgtacca cttcggcacg cgcaagctga cgttcctgaa gaaccggacg atcgttgagg
     3241 tggtcaactc gctgagcgac caccccgacg cccgctcggc atgccgcgcc gccggaatcg
     3301 acgacgcgca gcaggcgccc tatctgcacg cgctcggcgt gctggcccag tccaagatgc
     3361 tcgtaacggg agaagcatga cgtctgtagc accggttccc cgcctcgtcg agcagttcga
     3421 gcgtgggctc gacgcgccga tctgcctgac ctgggaactc acctacgcct gcaacctggc
     3481 ttgtgtgcac tgcctgtcat cgtcgggtaa acgcgatccg cgcgagctga cgacccagca
     3541 gtgcaaggac atcatcgacg agctcgagcg catgcaggtg ttctacgtca acatcggcgg
     3601 cggcgaaccc actgtgcgcc cggatttttg ggaacttgtg gactacgcca ccgctcatca
     3661 cgtcggcgtg aagttctcca ccaacggggt acgcatcacg cgcgaggtgg cggagcggct
     3721 ggccgcaagc gattacgtcg acgtgcagat ctcccttgac ggtgcgacgg ctgaggtcaa
     3781 tgacgctgtg cgcggccaag gttcgttcgc gatggcgatt cgtgcgttgg agaatctggc
     3841 cgaagccggc ttctccgacg ccaagatctc cgtcgtggtg actcggcaca acgtggacca
     3901 gctagacgaa ttcggcgctc tggcagcgcg ctacggcgcg atgctacgga tcacccggtt
     3961 gcgtccgtcc ggtcgcggtg ccgacgtgtg ggacgaactt catccgaccg ccgagcagca
     4021 ggtgcagctt tacgactggt tggtggccaa gggcgaccgc gtgctcaccg gtgactcctt
     4081 ctttcatctg tcgggcctgg gcgagcccgg cgcactcgct ggactgaacc tgtgcggcgc
     4141 cggccgcgtc gtgtgcctca tcgacccggt aggtgacgta tacgcttgcc cgttcgccat
     4201 ccatgaccgg tttctggcgg gaaacatcct gtcggacagc ggttttgaca acgtctggaa
     4261 gaacgcgccg ctgtttaggg agctgcgcga accgcagtcg gccggtgcgt gcagcagctg
     4321 cgggcactac gacagctgcc gtggcggctg catggccgcc aagttcttca ccggtctgcc
     4381 gctcgacggg ccggacccgg aatgcgttga gggttatggc gctcccgcgc tcgctcgaga
     4441 acgagtcaaa cccaagccaa gcgtcgacca ctcccgccgt gggcccgtga tgctgaagct
     4501 gctcaccaaa cccccggtcc gactctgtaa cgaaagcccg gtgtaagtca tggcacgcga
     4561 ccaatggttc gagacggtgg cgttctgcca gcaggcggcc aagaagcggc taccgaagtc
     4621 ggtgtacagc gcgctgctgg gcgcaagtga gagaggcgtc acggtcgccg acaacgtcga
     4681 cgccttcgcc gagctcggct tcgcgcccca cgtcataggc gcgctggaga agcgtgatct
     4741 gtcgacaacc gtgatgggac aagatatttc gcttccagtg atgatctcgc cgacaggagt
     4801 ccaggcggtc catcccgatg gtgaggtcgc cgtggcccgc gccgcagccg cgcgcggaac
     4861 ggcgatgggt ctgtcatcgt tcgccagcaa gccgatcgaa gaggtcatcg ccgccaaccc
     4921 caagttgttc ttccaggtgt actggctcgg cggccgcgac gcgatcgcgg cgcgggtgga
     4981 acgcgcccgg gaagcgggcg ccgtcggcct gatcgtgacg accgactgga gcttctcgca
     5041 tggccgggac tggggcagcc ccaagatccc ggaggagatg aacctgcgga ccacggtgcg
     5101 gatgctgccg gaggggctga cccgtccgcg ctggttcctg gattgggcca agaccatgcg
     5161 gccaccggac ctgcgggtac ccaaccaggc accgcgcgga gagcccgggc cgccgttctt
     5221 tgccgcctac ggtgaatgga tggccacccc gccgccgacc tgggaggaca tcgcctggct
     5281 gcgtgagctg tggggcgggc cgttcatgct gaagggcgtg atgcgcgtcg atgacgcgaa
     5341 acatgctgtg gacgcgggtg tttcggcgat ctcggtgtcc aaccacggcg gcaacaatct
     5401 cgatggaacg cccgcgtcaa ttcgagcact gcctgcaatt gccgacgccg tcggcgacca
     5461 gatcgaagtg ttgctggacg gtggcatccg gcgtggcagc gacgtggtga aggcgctggc
     5521 gctcggtgcg cgggcggtca tgatcggtcg cgcgtacctg tggggcctgg cggccaacgg
     5581 ccaagccggt gtagagaacg tgctcgacgt cttgcgcgga ggcatcgact cggcgttgat
     5641 gggtctgggc cgagcgtcag tccatgatct tgtcgccggc gacgtcctcg tcccgccgga
     5701 cttctcccga gccctcgggg tgccccccgc gtccaacgcc tgaccggcaa accgggccgg
     5761 cgcgaacgcc ctggtactgg cgtggcggag ggggctggcg tgattggtct gaggtgccag
     5821 aggaaaaatc acggaaatca attgctgcgc atgcgccaac aattggcgca cgccaggtga
     5881 attcggccta ccatcggcgg gtggccttcc ccagcgagct cgcgaactta acgtcgagac
     5941 agctgcagac cacgtcgccc gcgttgatta tccctgtcgg ttcggtcgaa cagcacggcc
     6001 cccacttgcc gctggacacc gatacccgga ttgccaccgc cgtcgctcgc ttgcttgctg
     6061 accggctcgc cgacgcgaat gaatccaact gggcgctggc acccgcgatc ggatacggcg
     6121 caagcgggga acacgagggc ttccctggca ccgtctcgat cgggacgtca gcgctgcggc
     6181 ttttgctggt ggagttcggc aggtccgcat cgcaatgggc gtcgcgcctc gtcttcgtca
     6241 atggacacgg cggaaacgtc gaggcgctgg cggccgcggt tgctttgctt cggtacgagg
     6301 gccgcgacgt ggcctggtgt tcgtgcaccg cggcgaacgc cgatgcacac gccggtcaca
     6361 ccgaaacgtc tgtattgcta catatttcgc ccgccgacgt atgggtcgac gagcgcctcc
     6421 ccggcaaccg tgagccgctg gcgcagttga tgccacagat gcgccagggc ggtgtcgccg
     6481 cagtgagcga actcggcgtg ctcggcgacc cgacgaccgc aacggctgag gaaggtgaac
     6541 ggatcctcgc cgagatggtc gacgcgtgtc tgcttcgtat cacccgctgg gcaccggatc
     6601 gcgacgggat gctgacatga cgggaccgcg gctgcccgat gggttcgccg tccaggtgga
     6661 tcgccgggtg aaggtgctcg gcgagggctc cgcgctgctc ggcggttcac ccacgcggct
     6721 gctgcggctg gccccggcag cgcagaccat gctcaccggt ggccggctcg aggtgcacga
     6781 cgcggtcagc gcacagctcg cgcgcacctt gctcgacgcc acggtcgccc atccgcgacc
     6841 agccactggc ccgtcacacc gagacgtcac cgtcgttata cccgtgcgcg acaacacttc
     6901 tggactgctg cgcctcgtcg gcacgctgcg cggcatgcgt gttgtggtcg tcgacgacgg
     6961 ttcggccaca ccggtgcaac agagcgactt cgccgacatg cactgcgaca ttcaggtgct
     7021 tcgccatacc cgtagcaagg ggcccgcggc ggcgcgcaac accggcctgg tggcgtgtac
     7081 caccgacttc gtggccttcc tcgactccga cgtggtgccg cggcgcggct ggctggaggc
     7141 gctgctcggg cacttctgtg accccgctgt cgcgctggtt gcaccgcgga tagtggggct
     7201 gcgtcaggcc gacaacctcg tggcccgata cgaggcggtg cggtcatcgc tcgatctggg
     7261 tctgcgggag gccccagtcg tgccctttgg cacggtgtcc tacgtcccca gcgcggccat
     7321 catctgccgc cgcgccgtga tcaaggacct gggtgggttc gacgaaacgc tcaagaccgg
     7381 cgaggacgtc gacctgtgct ggcgcttcat cgaggccggc gcccggctgc gctacgaacc
     7441 catcgcgctg gtcgcgcacg atcaccgcac ccaaatgcga gagtggttcg tgcgcaaggc
     7501 tttctacggc gaatcggcgg ccccactgtc tatccgccat cctggcaaga ccgcgccact
     7561 ggtgatctcc ggctggacgc tggtggtgtg gatcctgctc gccatgggat cgggcatcgg
     7621 atacctggcg tcgatggtcg tcgccgccgt caccgggcgt cgcatcgcga agtcattgag
     7681 caccgtcgag accgagccca aggaagtggc ggtggtcgcc gcgcacggcc tctggtcggc
     7741 cgccctgcag ctggcatccg cgatctgccg gcactactgg ccgatcgcgt tgatcgcggc
     7801 ggtgctgtcc cagcgttgcc gccatgtggt gctggtggcg gctgtcgtcg acggggtggt
     7861 ggattggatc acccgtaacg gcaacgccga cgacgacacc aagcgcgtcg gcctgctcag
     7921 ctacgtactg ctgaagcgtc tcgacgacat cgcgtacggc gccgggctgt ggaccggcgt
     7981 ggtgcgcgaa cggcacgccg gcgccctcaa accccagatc cggacctgac gccgcgttgc
     8041 acagtgacgt gctgatcgtc ggtgccggaa gcgctggatc tgttctggcc gaacggcttt
     8101 ccgccgaccc gcagtgccgg gtgaccgtcg tcgaggccgg acccgggccg tccgacccgc
     8161 gggtgctcag ccagatcaat gggctgcacc tgccgatcgg tgacgccagc tcagtggtac
     8221 ggcgataccc gacgacgttg accgacgacc ccaagcgctt agcgcagatc atgcgcgggg
     8281 cagtagtggg tggctcgggt gccgtgaacg gcggctactt ttgtcgcggt ctaccaatgg
     8341 atttcgacag ttgggggttg ccgggttggt catgggcaga cgtgctaccg cactttcgtg
     8401 cgattgaaac cgaccttgac tttgacaatc cattacacgg gtcagacggt ccgatcctcg
     8461 tccgtcgtgt tgacgaattc gacgggtgca cagcgtcgtt cgtacgatca gcaggtgaag
     8521 ccgggtaccg gtggatcgac gacctcaacg gcgccacgcc ggaagagccg ttgccgccag
     8581 gtgtcggcgc ggtgccgctg aacatcaacg gcgggacccg ggtaggcccg ggtggcgcgt
     8641 acctgcaacc tgcgatggac cgtaccaacc tcacgctgct gaccaatacc agggttcgac
     8701 gagtgcggtt gaccggtggc agggccgtcg gcgtggagtg cgtcggaccc gacgggtccg
     8761 gcgatctgac cgcggatcgg atcgtgctgt ctgccggggc aattggctct gcgcagctgc
     8821 tgatgttgtc cggcttgggt ccgcgggagg tgctgcgagc agcagaggta ccggtggtgg
     8881 cggacctgcc cgtcggtgtg gcatgtgtcg atcatccgga atgggtcttg ccggtcgact
     8941 ggtcagaaac ccacgggctg ccgccgctgg aagcggtgct gacgaccgac actctcgaga
     9001 tccggccata cactgcagga ttcggtgcca tggtcagtgg gcggcgggat gatccgggcg
     9061 accatccgca catcggtgtg gcgctcatgc agccggagtc acgtggccgc gtggccgtga
     9121 cgtcagcaga tccggacacg cctccgctga tcgaacaccg ctatgaccat gcgcctgctg
     9181 acgtcgccca gctcgatgcg ggagccgagc tggcgcggga attggtcggt gcgacagcac
     9241 aactgggacg ggtgtcatgg tcgacgtcgc aacacctttc gagcaccgcg ccgatgggtc
     9301 tcgacgggga tgcgcatgcc gtcgtcgacc agcagtgccg tgttcgcgga gtcgagaacc
     9361 tatgggtcgt cgacgggtcg gtcctgccgg ggatcaccag ccgcgggccg cacgccacga
     9421 tcgtgatgat tgggcaccgg gcggccgagt tcatcgtcgg ctagtggcct ccgcctgagg
     9481 aagccgaact gccgaccgtc gcgacccggt gcccacacgg cgacgaacgc cgcggcgacg
     9541 gccaacacga tggacaccac tagcaacgac aggtccatcg agtcgatgaa cgcagactcg
     9601 gcgagttccg ccaatcgcgc tccctgcgga cccatctgag ccgcgacggc gagggcatgg
     9661 gcgagggagt cgagtgccga ctcgcgaaca tgctggggaa actccgcgac gccgggcgag
     9721 agcagattgt ggtattgggc ggcgagcaca gatcctgcga ctgcgatgcc gacggcggcg
     9781 ccgacttccc gcgtcgtgtc gttgaccgcc gacgcgaccc cctgcttctc ggtgggcacg
     9841 gcgttcatga tcgccgaagt cgttggtgcg acacataaac cgatgcccgc gctggtgatc
     9901 aggagcggcc agacgaggtc gaggtacgtt gacccggcat caaggaagcg catggagaac
     9961 aggcccgcgg cgatgaggaa caatccaagc gacacggcga cccgaaggcc tatcctcggc
    10021 agatagagat gcattgtcgc gccgagaacc atgattggta ccgccaatgg cgtcagagcg
    10081 aaggccgttt gaagcgggct gtagccgagg atcagctgca tgtattgcat ctcgacgaag
    10141 aagaatccga agttggcgaa gaacagaagc gttatgccga tcgagccggt ggcgaaatcg
    10201 ggcctgccga acagccttac gtcgagaagg ggatgcttgc gccgcagttc gaccgccgcg
    10261 aacgcgccgg ccagcgcgac acccgccagc atgcagcccc acacaatcgg atgtgtccag
    10321 ccgcgcaccg gcgcttcgac tacgccgaac acgaacaccg cgacggcacc gccgatgagc
    10381 accgctccaa gccagtccag cggcgtagcg gtttcgtcac gcgacgaggc gatcgtgcag
    10441 gtgcaaagaa gcaatccgag tgccgcggcc gcgaacccgt aaaagatgga ctgccacgag
    10501 aagaacttca gcagcagtcc ggtgcccatg aacccgacaa tcgccccgga gctcgcgaca
    10561 ccggcccaga tgccaacggc cttgttgcgc tcggacttcg ggaacgcagc ggtcaacagc
    10621 gacagcgtcg cgggcatgat gaatgccgcg ccgatacccg cgaccgctcg ggcgacgatg
    10681 atctgaactg gactgtcgaa gatggtcggc gacagcgagg cgaccgcaaa aatggcgagg
    10741 ccgacgagca gagcgccgcg gcggccgtac cggtcgccga gcgcacccgc aggcagaagc
    10801 agacacgcga gcaccagggt gtagccatcg acgacccagg tcagttgcgt ttggctggcg
    10861 gaggtctgta gcgcgatgtc aggaagcgcc gcgttgaggg cgaccatcga cgagatgacc
    10921 aacaacacat cgagcgcagc gaccgtcaac aaccagaagc gtgcgcgctt ggacaaggtg
    10981 gcggcgctgg cgtcgatatc ctgatcagac cgtgcgaggg tgtcgaccat tggcgtcgct
    11041 ccttcggtgg atactttcga gactaacagt ctcggtccga gaccgatagt cttgttccct
    11101 cgttggaggt gatgtaaacg atgaccgcca taggtaacga tccacggcct gcccgctcac
    11161 gggctcgttt gctcgacgcc gcgacagcgt tattgcgatc gggcggccca agcgcggtca
    11221 ccatcgatgc ggtgacccgt agcgccaacg tcgcgagggc gacgctgtac cgccatttct
    11281 ccagcgccaa cgatctgctt gcggccgcgt tcacgggcct gctgcagcct gcgccgatgc
    11341 cacccgctga aggctccttg cgtgatcagc tgattgcggt ggtggtggga tgggctgagt
    11401 cgattgccga ggtgcccacc accatcacgg ccatgacgtg gctggcgtcg ggaccggatg
    11461 caggagcgta ttccgaagtg cgccagaccg attcagacgc cgttggtact ttgcgggcca
    11521 ggatcatcca gatgtactcg gcgccgttcg acgccatctt cgacagtccg caggccgctg
    11581 ccgagctcgg cgacgtggac cgtgttcagg caatcgccct gctcatcggg ccgctgatcc
    11641 tcggaaggct ctccacgctt gccgatttcg actaccgcac gtgcgccggg gccgccgttg
    11701 acggttttct ggccacccac gcgaaggcga gaaacgtcac ctcagcgagt agcggatcgg
    11761 caggtgcttc aggccgccga caaaagtagt ggatgccagt tcgggctcac cggccaactc
    11821 gatcgactcc agccggggca ccagctcgga gaagaagctg ttgatctcca tccgggccag
    11881 cgccgctccc aggcagaagt gcacgccgta gccgaatgcc acgtgtttgt tgggatcccg
    11941 gccgatgtcg aacctgaatg ggtcatcgaa gacttcttca tcgcggttgc ctgaaacgta
    12001 agcgagatat acggattcgc cttcagcgat cgggacatcg cgcactgtcg tatcggccgt
    12061 cgccgtccgc atgaactcct tgaccggcgt cacccagcgg atcatctctt cggtggcggt
    12121 gcccagcagt tgcggattct gctgcaaccg ttggagctcg ccagggttat cgatcagcgc
    12181 ccgaagtccg cccgcgatcg tggcgctcgt ggtgtcatgt cccgcagtgg cgatgatgac
    12241 gtagtaggac atcgtctcca ggtcggacaa tggttggccg tcgatacggg cgttggcgat
    12301 cgccgacgcc agatcctcgg tgggatgttc gcggcgcgac gcggtcaacg aggtgaagta
    12361 gccgaagaag tcgagcaaca ccgcattctg gtcttccagc gtggtgccgc gcttgtactc
    12421 ctcctcgtcg cctccgaata gctcttgggt gagcttgagc atccgtggga agtccgactc
    12481 gggcaggccg agcagcgaca ggatcacata cagcggaaag tgcacggcga cctcgtggac
    12541 gaaatcgcat tcgccgccgg ccgccaccat cttgtcgacg tgaatcttgg cgagttcgtc
    12601 gacacgaatc ttcaagtcgc gcatggcttt cggacggaac cagtcggcgc cgatggcacg
    12661 gatcacccgg tgctgcgggt catccatatg gatcaacgtg cgcaggccgc cgccggcctg
    12721 aagcatttcc tgcgccatat cgtctgcgtc cgcggtggcc agcagcggcc ggggctcgtt
    12781 gatccacaga gtgttgtcgc gctcgatggc catgatgtcg gcgtgcttgg tgacggccca
    12841 gaatggccgg tacggcggat tgtcgaccca ggcgaccggg ttgttcgccc gcagatgggt
    12901 cagcgccgca tgcagtcgcg cgtcgtcggc ataggccgtc ggatcggcca agacctttgc
    12961 ggcgtcatcc atcgtcggcg tgctcattcg ggctcctcac tgcgagctga cagaacttga
    13021 cgggtgtcaa gttcgcagtc tatgtgaccg ccgccacttt ggggaagaga ggtgtggatc
    13081 taccaccact aggctctaac gtcgtgccgg cccgatcgct tcaccgcctg ttggcgcttg
    13141 ccttcgtctt gttgctggcg gtggggtgta gtcgcggcgg cggtgagtct ccgaagacgg
    13201 cggccccgac gcatcagtcg gatccggcgg tggtgcagac tgccgacggg acgctgcggg
    13261 gcatcgtcgc gccagaccac cggctgttcg ctggaatccc ttatgccgca ccgccgatcg
    13321 gtcccctgcg atggcagccg ccggatcctg cggcgccatg gcagggggtg cgcgacgcca
    13381 cccgcgtcgg accacgctgc ctgcaggatc cgggcggtga cctggaatta gggcggcaaa
    13441 ctgacgagga ttgcctgacc ctcaacgtct ggacgccgcc ggtcgacccg agccgcggcc
    13501 agcggccggt gatggtgtgg atccacggcg gctcgttcgt caacggcagc ggcgggatct
    13561 atgactcgcg ctggctggca gcccggggcg acatggtcgt cgtcaccgtc aactaccggc
    13621 ttggcgcgac cgggttcctg gcgcacccgg ctctcggtcc cgcgggtgac gtcggcaatt
    13681 acggcctggc cgaccagcag gcggcgctgc gctgggtgcg cgacaacatc gccgacttcg
    13741 gcggtgatcc ggccaaggtc acgctggcgg gtgagtccgc cggcggcatc tcggtatgcg
    13801 accacctggt tgcgccggat tcggctgggt tgttcagagc ggcgatcatc caaagcgcac
    13861 cgtgccaggc gcaactcgca ttgccggcgg cggagaagag cagtctggac tatgcggcta
    13921 cagttggctg cgccgatccg caatccgcag cgcagtgtct gcgagcgttg cccgccggca
    13981 agctacgaga cccggtctgg tacttcaaga ttggcgatga tgccctgagc ggtccggtga
    14041 ccgatacgag atcgttgccg gtggacccca tggccgcctt cgccgacggg cgttctgccc
    14101 gcgtgccggt attgatcggc accaaccacg acgaattcac gttgttcgtg gcgctgcagt
    14161 atttgcggct gggccgggaa tacacgaccg atcagtaccc acaattgctg cgcgagacct
    14221 tcggcgacta cgccgccgcg gtcgaggagc attacccctt gcagcgctat agcagtgtgc
    14281 cccttgcata ttcggcggcc gtcacggacg gagtgttcgc ctgtgtgtcc gatcggatgg
    14341 ccaaggcgat ggcgcggact caacccgtct acgcctacga gttcaacgac cgcaacgcgc
    14401 cgacctccga gccgatgcgc acgctgccat ttccggtggg cgccagccat tctctcgagt
    14461 tgcgctacct tttcgacatc ggcgacgcgc agccgctgaa cgccgcgcag caggtgttgt
    14521 ccgaccagat gatcgactac tgggcgcatt tcgtgcacac cgcagatccc ggctcgcagt
    14581 ggccggcgtt cggtcccgac gaaaagcggt tgtcgtttca gtctgacggg agcagagtga
    14641 ccaccgacta cgacaaaacg caccagtgcg cattctgggc gagcctgaaa ggttgactgg
    14701 catgccgact cccgacccga aaagctatgc ggatcagtgg gtgcgggcgt ggaatggaca
    14761 cgatatcgag gcggtgcttg accactttca cgacgacgtc gtgttcacgt cgccggtagc
    14821 cgtccgggtg gtgcccgaaa gcgggggagt ggtgcacggc aagcccgcat tgcgggacta
    14881 ctggaccgct gcgttggcca agcagcccga cctgcagttc gacgtcgttg gcgtctaccg
    14941 cggcgagtcc acgttggtga tcaactaccg caatcaccgg ggcgaactcg tcaacgaggt
    15001 gctgaccttc gacggcgaca aagtgcgcga aggccacgga acgtatctgg cttaggccgg
    15061 cgtcacccgc gtcatgatgt cggcgtggac gacctctgtg cctgacttgt cgacgacgct
    15121 gacgggcacg acgatctccg cgccctcggt gatcgaggcg aagtccggtg gctccagccg
    15181 ggcctgtgcc cgcagcgacg tcgtcgcctt ctcgcggtac tgcacattca tcgccttcgg
    15241 gatccagtgg tggctggtcg gcacggtggc ctccataagc attcccatcg cgatctccgc
    15301 ggcgttgcac gacgcgatcg catgcacggt gtgcagatgg ttgtagacga agaacattcg
    15361 ggtgatgctt taggccacat gcggacagcc gcctacacca aaatcagcca ggacgccgcc
    15421 ggtgagcgtg ctggcgtggc ccgccagttg gaagactgtc tggcattcgc cgaccggctg
    15481 ggctgggatg gcggctgcgg cggcagcggc cccaacgtcc tctgttgagc cgacagaacc
    15541 gtgcaatcat ggcggcagcc gccgctcggc gtacctctga tctgaaatag tcggcccgcc
    15601 accaaggagc gggtggcggg ccgaacacta gatcagacgg tgaatggtgt tatccggttc
    15661 cgccctcgac ttgattggcg acgtgaagca atccggtggc cagttcgcgc tcctcgtcgg
    15721 cagtcaggtg caggcagggg caggcaggca aaacggggag ccccgcacga gactccccgt
    15781 tcggttgagc ggtctgctac ccgcagatgc cggggctgac cgtgacgtgc gcggcctggc
    15841 cggtgaacga tgttccgcac gccgacaggc tggtggcgga actcgcgtta cctgaggccg
    15901 tgaccacgcc gccgacgttc acgagggtgt tgtcgtgggc gttaccggat accgaagacg
    15961 ctcccaacac cgagacgacg ttgttgccgg tggcattacc actcgccgtg gagctgccgt
    16021 cgatggcaag caggttgttg ccgctggcgt tgccctgcgt ggtggccgag gccgtcccga
    16081 agacgctgat cgcaaccccg gtattgccct cgctcacatc ggccgtggcc ccggtgccaa
    16141 cgctgaagcc atcgcccgcg ccgggatcaa cagcgaagcc gagagcggaa gccgtcccgg
    16201 caccgaacat tccggccacg cccagacctg cggcgagaac cgatcctgct attaccttgt
    16261 gaaccttcat gtcgcaattt ccttactgtg agagttgga
//
LOCUS       DASRJA010000655         3601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2258369,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000655 DASRJA010000000
VERSION     DASRJA010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258369"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="VFC01_29510"
     CDS             <1..1203
                     /locus_tag="VFC01_29510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZN83788.1"
                     /translation="GAALPLLFFALAGRRVAERVASFRRRQREIRIAAGVVTILLAVA
                     LVLNLPAALQRAIPDYTSKLQETVGGDEQLREKLNLGGLVNDQNRELSNCTNGAPELE
                     SCGTAPDITGITGWLNTPNGSAIDLKSLRGKVVLVDFWAYSCINCQRAVPHIVDWYAA
                     YKDAGFEVIGVHTPEYAFERVPANVASGARDLGITYPIALDPSYSTWTNYRNRYWPAK
                     YLIDANGTVRHIKFGEGDYAQTETLIRQLLSDAKPGVTLPKPTEAADTTPPPDLTPET
                     YLGVGKVVNYGGNGRYDEGSATFGYPGRLPDDTFALQGDWALDYQGATANAPNSSIKL
                     NYRARNVYLVVGGTGTVTVIRNGESKDIPVSGPPTLRQIVGDDEIERMLVEVRLSQGL
                     QAFSFTYG"
     gene            complement(1205..1699)
                     /gene="msrB"
                     /locus_tag="VFC01_29515"
     CDS             complement(1205..1699)
                     /gene="msrB"
                     /locus_tag="VFC01_29515"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZN83789.1"
                     /translation="MGVTRRRFLVGVGAASATILTAAACTRSATASPAGDLEVTHTDA
                     EWKQLLNGDQYDVLRRAGTERPYSSPLNEEHRRGTFSCAGCALELFSADTKFDSGTGW
                     PSFWQPLDGAVLERPDDSIGMTRTEVLCRRCGGHLGHVFDDGPPPTGLRYCMNGVAMT
                     FRPA"
     gene            1843..2547
                     /gene="msrA"
                     /locus_tag="VFC01_29520"
     CDS             1843..2547
                     /gene="msrA"
                     /locus_tag="VFC01_29520"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZN83790.1"
                     /translation="MRLTAKMGGLAALAVLVALGFVLFPASASTTIPAPVLDEPAGSP
                     GASETAVLAGGCFWGVQGVFQHVKGVSQAVSGYAGGEAATAQYETVGTGTTGHAESVQ
                     ITYDPSQVSFGQLLHVFFGVVHDPTELNRQGPDVGEQYRSAIFPQNDVQKKVADAYIA
                     QLNQARVFDGPVVTRTDKDSGFFPAEDYHQDYVNSHPTAAYIAINDMPKLADLRQQFP
                     ELYRDQPVLVHVVTHE"
     gene            complement(2534..3379)
                     /gene="sigI"
                     /locus_tag="VFC01_29525"
     CDS             complement(2534..3379)
                     /gene="sigI"
                     /locus_tag="VFC01_29525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigI"
                     /protein_id="HZN83791.1"
                     /translation="MSDSLSVEAAWRSHRAYLVNLAYQMLGDVGAAEDVAQEAFLRLS
                     RADPSQIDDVRAWLTVVAGRLCLDQLRSARARHEQPDVSGALDVTPSLEADPADRVTL
                     DDEVRTALLEVLRRLSPGERVAFVLHDVFQMPFEEIAETVGRPVGTCRQLARRARAKF
                     AGASPRPADVTEVEHRIVAEKFITACANGDLQSLAAVLDPTVWGVATFVGGGPPQVNH
                     GRDTVAPNLLRFLGHGMTLVTGPAGEVLGFAGHALSVVLVLTVRGDRVIKIEATVDPF
                     AATRG"
     gene            3461..>3601
                     /locus_tag="VFC01_29530"
     CDS             3461..>3601
                     /locus_tag="VFC01_29530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZN83792.1"
                     /translation="MNAILVTGATGNVGRPLVNQLAAAGVEVRAVSRHPDTARFPDGV
                     TVV"
BASE COUNT          661 a         1237 c         1126 g          577 t
ORIGIN      
        1 ggcgccgcgc tgccactgct gttcttcgcg ctggccggtc gccgtgtcgc cgaacgtgtg
       61 gcgtcgttcc gccgccgcca gcgtgaaatc cgcatcgccg caggcgttgt cacgatcctg
      121 ctcgccgtgg cgctggtgct caatctgccc gccgcgctgc agcgtgccat ccccgactac
      181 acgagcaagc tgcaggaaac cgtcggtggc gacgagcaac tgcgcgagaa gctcaacctc
      241 ggcggcctcg tcaacgacca gaacagagaa ctgtccaact gcacgaacgg cgcgcccgag
      301 ttggaaagct gtggcacggc gccggacatc accggcatca ccggctggct gaacaccccc
      361 aatggctccg ccatagacct caaatcctta aggggcaagg tcgttctggt ggacttctgg
      421 gcgtactcgt gcatcaactg ccagcgtgcg gtcccgcata tcgtcgactg gtacgccgcg
      481 tacaaggacg ccggcttcga ggttatcggc gtgcacacac cggagtacgc gttcgaacgc
      541 gtgcctgcga acgtcgccag cggcgcccga gatctcggca tcacctatcc gatcgcgctc
      601 gaccccagct actcgacgtg gaccaactac cgcaacaggt actggcccgc caagtacctg
      661 atcgacgcga acggtaccgt ccggcacatc aagttcggcg agggtgacta cgcacagacg
      721 gaaaccctga ttcgccaact gctttcggac gccaagcccg gcgtcacgct gccgaagcca
      781 accgaagccg cagacacgac tccaccaccg gacctgacgc ccgagacgta tctgggtgtc
      841 ggcaaggttg tcaactacgg cggtaacggc aggtatgacg agggcagcgc cacgttcggc
      901 tatccgggcc gtctgcccga cgacaccttt gccctccaag gcgattgggc gctggattat
      961 cagggcgcga ccgccaatgc cccgaactcg agcatcaagc tgaactaccg tgcccgcaac
     1021 gtctatctgg tggtcggcgg aaccggcacc gtcacggtca tccgcaacgg tgagtccaag
     1081 gacatcccgg tcagcgggcc gccgacgctg cgccagatcg tcggcgacga cgagattgag
     1141 cgcatgctgg tagaggtgcg gttaagccaa gggctgcagg cgttctcgtt cacctacggc
     1201 tgaatcatgc tgggcggaag gtcatcgcaa caccgttcat gcagtagcgc aggccggtgg
     1261 gtggtggacc gtcgtcgaaa acatggccga ggtgaccacc gcagcgtcga cagagcacct
     1321 ctgttcgtgt catgccgatg ctgtcgtcag ggcgctcgag tacggcaccg tccagcggct
     1381 gccagaagct cggccacccg gtgccgctgt cgaatttggt gtccgccgag aacaactcca
     1441 gtgcgcaacc ggcgcagctg aaagtgccac gccggtgttc ctcgttgagc ggactgctgt
     1501 atggccgctc ggtgccggct cggcgcagaa cgtcgtactg atcgccgttc agcagttgct
     1561 tccactcggc atcggtatgg gtgacctcga ggtcgccggc cgggctggcc gtcgccgaac
     1621 gcgtgcacgc ggcagccgtg aggatcgtgg ccgacgccgc gcccacaccg accaggaatc
     1681 gccgtctcgt cacgcccatc ccacccggcc tccgtcaacg tgttgtcctc gggcgaatct
     1741 aaaccgcaat ggcgtcctta cccgttgcag tagaaagcag tgtcacagtt tagtaaggaa
     1801 tgatccgacc gcgtgcgcaa cgaacgtcgc taaccttgcc ggatgaggtt gaccgccaag
     1861 atgggcggac tcgccgccct ggccgtgctg gtggcactgg gtttcgtgct gttccccgca
     1921 agcgcctcga ccaccattcc ggctcccgtg ctcgacgaac ccgcaggcag cccgggcgcc
     1981 agcgagacgg cggtgctggc gggcggctgc ttctggggtg tccagggcgt gtttcaacac
     2041 gtgaagggtg tgtcccaggc ggtgtccggc tacgccggtg gcgaagcagc gaccgcccag
     2101 tatgagacgg tcggcacggg taccaccggg cacgccgagt ccgtgcaaat cacctacgac
     2161 cccagccagg tgagctttgg acagctgctc cacgttttct tcggcgtcgt ccacgacccg
     2221 accgagctga accggcaggg ccccgacgtc ggtgagcagt atcgctcggc gatctttccg
     2281 cagaatgacg ttcagaagaa ggtcgccgac gcctacatcg cgcagctcaa ccaggcgagg
     2341 gtcttcgatg ggcccgtcgt cacgcgcacc gacaaggact ccggcttctt ccctgccgag
     2401 gactaccacc aggactacgt caactctcat ccgacggcgg cgtacatcgc gatcaacgac
     2461 atgcccaagc tagccgacct gaggcaacag tttccggagc tgtaccgcga ccaacctgtc
     2521 ctggtgcacg tggtcaccca cgagtagccg caaaaggatc gaccgtcgct tcgatcttga
     2581 tgactcgatc accgcgcacc gtcagcacca gcaccacgga aagcgcatgg cccgcgaagc
     2641 cgagcacctc gcccgcaggc ccggtgacca gagtcatacc gtgaccgagg aaccgaagga
     2701 ggttgggcgc caccgtatcc cgtccgtggt tgacctgcgg cggcccgcca ccgacaaacg
     2761 tcgccacccc ccacaccgtg ggatccagca ccgcggccag ggactgcagg tcgccgttcg
     2821 cgcacgcggt gatgaacttc tcggcgacga tccggtgctc gacctccgtg acatcagccg
     2881 gcctcggcga tgcgcccgcg aacttcgccc tagcccgtcg cgcaagctga cgacatgtgc
     2941 cgaccggcct gccaaccgtc tcggcgatct cctcgaacgg catctggaac acgtcgtgca
     3001 ggacgaacgc cacgcgctca ccgggactca gccgccgcaa cacctccagc agcgcagtgc
     3061 ggacctcgtc gtcgagcgtg acgcgatcgg cggggtcggc ctccagcgac ggggtgacat
     3121 cgagagcccc tgacacgtcg ggctgttcgt ggcgggcgcg cgcggaccgc agttggtcaa
     3181 ggcagagccg gcccgcgacg acggtcagcc aggcgcggac gtcgtcgatc tgtgacgggt
     3241 cggcgcgtga aagccgcagg aatgcctcct gcgcgacgtc ctcggccgcg ccgacatcgc
     3301 cgagcatctg gtacgcgagg ttcaccaggt atgcccgatg gctgcgccac gccgcttcga
     3361 ccgacaacga gtcgctcatg cagtaatgac gaaacggcga agcgaaaagt tacccgggcc
     3421 gctgtgtaac ttttgccgtc gcggcgtcgt ccttgtctcc atgaatgcaa ttctcgtcac
     3481 gggcgcaacc ggcaacgtcg gccgcccact ggtcaaccaa ctcgcagcag ccggagtcga
     3541 ggtgcgcgcc gtcagccggc atcccgacac cgcgcggttc cccgacggcg tcacggtggt
     3601 g
//
LOCUS       DASRJA010000656         8895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1213717,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000656 DASRJA010000000
VERSION     DASRJA010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213717"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="VFC01_29535"
     CDS             <1..148
                     /locus_tag="VFC01_29535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN83793.1"
                     /translation="GGLVKDASNYNNTPVVLVGIFAIGISGLVIDGLLRAVERRAVPW
                     RGKI"
     gene            148..1176
                     /locus_tag="VFC01_29540"
     CDS             148..1176
                     /locus_tag="VFC01_29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZN83794.1"
                     /translation="MKFKALIVVLAASALALAGCSVDRSGDRADKPTIRIGYQSFPSG
                     DLVVKNNKWLEEALPNYNIKWTKFDSGADVNTAFIAKELDFGALGSSPVARGLSAPLN
                     IPYKVAFVLDVAGDNEALVARNGSGVNTIADLKGKRIGTPFASTAHYSLLAALSQNGL
                     SANDVQLVDLQPQAILAAWDRGDIDAAYSWLPTLDQLRKTGKDLITSRQLAKNGKPTL
                     DLGAVRDEFATAHPDVVDIWRQQEARALKVIHDDPAAAAKAIAAEIGLSPGEVAGQLK
                     QGVYLTPEQVVSPEWLGSDAKPGNIAVNLQSASQFLADQKQIPSAAPLQTFEAAIYTK
                     GLPGAITQ"
     gene            1160..1939
                     /locus_tag="VFC01_29545"
     CDS             1160..1939
                     /locus_tag="VFC01_29545"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN83795.1"
                     /translation="MPSPSDGTIRISNVKHRYGRGRDEVTALGPVDLTVDPGSFLVLV
                     GASGCGKSTLLRLLAGFESPSEGTVAVSGAAPTPGVTAGVVFQQPRLFPWRTVGGNVD
                     LALKYANVPRERRAERRDELLARVGLEGTVKRKIWEISGGQQQRVAIARALAAETPLF
                     LLDEPFAALDALTRERLQEDVRQVSAESGRTTVFVTHSADEAAFLGSRIVVLTRRPGQ
                     VALDLPVDLPRTGVDPDELRRSAEYGELRAEVGRSVKAAAA"
     gene            complement(1943..2053)
                     /locus_tag="VFC01_29550"
                     /pseudo
     CDS             complement(1943..2053)
                     /locus_tag="VFC01_29550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
     gene            2193..2846
                     /locus_tag="VFC01_29555"
     CDS             2193..2846
                     /locus_tag="VFC01_29555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN83796.1"
                     /translation="MSEATANGMSRRDELLTVATKLFAARGYHGTRMDDVADAVGLNK
                     ATVYHYYASKSLILYDIYKGAADFTVDALHDDPTASARETIYHFTRRLLVGIAGNIER
                     AAVYFQEGPYITEWFTEDQVAYIREKETQVYEHVRDVIDRGIASGEFYDCDSHVLALG
                     YIGMTLGSYRWLRPQGRRTAQEIAEEFSTALLRGLIRDETVRAESPLGVDTGQLPTG"
     gene            2843..3583
                     /locus_tag="VFC01_29560"
     CDS             2843..3583
                     /locus_tag="VFC01_29560"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HZN83797.1"
                     /translation="MRQPTRDGSRRVGGGGRRATARAGGVGPAEHEKAYLKSALEHYG
                     VPVPAIRAVAKGVRKLIPDLSHDELVDVVEALWSAPVHERRMVAVELLDAYADRLVPA
                     DMVLLERLLRESRTWALVDGLAGSVVGPLVERAPTAAAVLDRWAVDDDFWIRRSALLA
                     LLESLRRGGGDFERFGKYADAILDEKEFFIRKAIGWVLRDTARKRPDVVFEWLLPRAA
                     RASGVTLREACEPLSAEQCAAVEAARRG"
     gene            complement(3585..4403)
                     /locus_tag="VFC01_29565"
     CDS             complement(3585..4403)
                     /locus_tag="VFC01_29565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN83798.1"
                     /translation="MTTTKVDFSSVGWRSVEWTNLVTLYLRACESRSPLPILGDHAAA
                     EAVDRIDYDFKRVHRAAQPWANQFLVALRAKKLDDWAAAFLRRHPDAVVLHLGCGLDT
                     RAFRINPPPTVQWFDLDQPGVIELRRKLYDETDTYRMIGSSVTDPGWLDQIPTGRPTL
                     VVAEGLLMYLTEREVWELLERLTDRFGSGELLFDTLSPAGPRLSKIATKGIVKWGIRD
                     ARAIEAWGLGLRFVEQVSAVAGYESIPFTPQRLVYRLMYATPMRNYDVVNRFAF"
     gene            4526..6322
                     /locus_tag="VFC01_29570"
     CDS             4526..6322
                     /locus_tag="VFC01_29570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN83799.1"
                     /translation="MKSNLLSRRDLDFLLYEWLRIDELTKRERFAEHSRETFDGVLDL
                     CEQLATRYFAPHNKKSDANEPSFDGDKVTVIGEVKDAWDAFAQAELMAMSMDQRLGGA
                     QLPATIAEAGFAWFSAANVSTTGYLMLTMANANLLAKFGTAEQVEAFLKPMLAGRFSG
                     TMALSETQAGSSLADILTRAEPQDDGTYRLFGSKMWISGAEHELTENIVNLVLAKIPG
                     GAPGTKGISLFVVPKFLVDADGSVGERNDVTLAGLNHKMGQRGITNTVLNFGEGRFTP
                     GGKPGAVGYLVGEPHRGLAYMFTMMNEARLGVGMGAVSLGYTGYLKSLEYARGRPQGR
                     PVLAKDPSAPQVPIIEHADVKRMLLAQKAYVEGGMGLLLYCAKLVDLAHSAESEEERD
                     STGLLLDILTPVGKSWPSQWCLEANNLAIQVHGGYGYTREYDVEQHYRDNRLNPIHEG
                     THGIQSLDLLGRKVTQRGGASLAALGDAVAATVATANAAGGEAAELAAQLDATWQRLV
                     AVTTAMFGSGDVEAALANSVVYLEAFGHIVVAWMWLEQLLAANGRTGDFYDGKRQAAR
                     YFFRYELPKTAPQLDLLESLDRTTLEMRDSWF"
     gene            6449..7471
                     /locus_tag="VFC01_29575"
     CDS             6449..7471
                     /locus_tag="VFC01_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZN83800.1"
                     /translation="MVITNAFRRPRLSAALAATAAAGLVLTACGGVQTSTGAGSGGGN
                     YPNGTVEMYVGASPGGSSDLISRAVSKGLNDNLHASFPVINREGANGALAAAEVSKAK
                     PDGSTIAIQNASLFAITPLAVSPDEVTNIDDFDVVYGVSRDDYVLVTNPASGFKTLKD
                     LENANRVARYGTTGVGTGAQLAAALLFKSTNVESQAVPFDGGAPALAAVLGNQIDVAS
                     LQVGEAIENIQSGKLVPLSVFGPERIEYLPNVPTAKEQGLDVEVAQYRFMTVPKGTPK
                     EVQDKLIEGMQATFKTEDYKAFNKQNSLTPMEQPGGEVLAQLQADQKRYADMVKQYGI
                     SLRDEG"
     gene            7475..8005
                     /locus_tag="VFC01_29580"
     CDS             7475..8005
                     /locus_tag="VFC01_29580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HZN83801.1"
                     /translation="MNDGPAHTRDILAEIEAEVTHELEEERPQPGGPAYQTVGALIGI
                     AIGVGGAVLACGYGLGSLHEPGPGLWPFVVSVLIAALSIVLLVVGRGLTDSEAFTRSS
                     VLPVVGVATFIAFGLLMPLTGFEIPALALCVIWLRFLGGESWRNTIVISVLTTAVFYF
                     LFLYGLRIPLPHLIAF"
     gene            8011..>8895
                     /locus_tag="VFC01_29585"
     CDS             8011..>8895
                     /locus_tag="VFC01_29585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZN83802.1"
                     /translation="MDFNAFLNGFVVVVEPHNLLYCLIGVLIGMVIGVLPGLGPAATI
                     AILLPITYTIDPVSAIIMLAGIYYGAQYGGTVTSVLLRLPGEASSVVTVFDGFALAKQ
                     GKAGTALGIAAIGSFVGATISIIGLTLLAPVVAGIALDFGPPEYAALALLGVLLVATI
                     GSGNKLKALIAGAVGLLLATVGRDNFSGASRFTFDSLQLADGIDFVVVAMGLFGVGEI
                     LYNLEQRHRRVQPPAKVGNVWPSRKDIKQSSGAIGRGSFIGFVLGVLPGGGAVLSSIV
                     AYAMEKRRSKTPERFGRGA"
BASE COUNT         1561 a         2897 c         2947 g         1490 t
ORIGIN      
        1 cggcggcctt gtcaaagacg cgagtaacta caacaacacc cccgtcgtgc tggtcggcat
       61 cttcgccatc gggatctccg gccttgtaat cgacggtctg cttcgcgccg tggaacggcg
      121 cgctgtcccc tggagaggaa agatctagtg aaattcaagg ccctaattgt ggtgctggcg
      181 gcttcggcgt tggcgctggc gggctgctcg gtcgaccggt caggggaccg agccgacaag
      241 ccgacgattc gcattggcta ccaaagcttt ccgagcggcg acctggtcgt gaagaacaac
      301 aagtggctcg aagaggcgct gccgaactac aacatcaagt ggacgaagtt cgactccggc
      361 gccgacgtca acaccgcgtt catcgccaag gaactggact tcggcgcgct cggctccagc
      421 cccgtcgcgc gcgggctgtc ggcgccgctg aacatcccct acaaggtcgc gttcgtgctc
      481 gacgtcgcag gcgacaacga agcactcgtg gcgcgcaacg gcagcggcgt caacacgatt
      541 gccgatctca agggcaagcg gatcgggacc ccgttcgcgt cgacagcgca ttacagcctg
      601 ctggccgcgc tgtcacagaa tgggttgtcg gccaacgacg ttcagctagt cgatctgcag
      661 ccgcaggcca tcctggcagc atgggaccgc ggcgacatcg acgccgccta ctcctggctg
      721 cccaccctcg accagctgcg taagaccggc aaggacctga tcaccagccg ccagctggcc
      781 aagaacggta agcccacgct ggacctcggc gcggtgcgtg acgagttcgc caccgcgcac
      841 cccgacgtcg tcgacatctg gcgtcagcag gaagcccgcg cgctcaaggt cattcacgac
      901 gatcccgccg cagccgcaaa ggccatcgcc gccgagatcg ggctgagccc gggcgaggtg
      961 gcgggccagc tcaagcaggg cgtctacctg acgccggaac aggttgtctc tccggaatgg
     1021 cttggctccg acgcgaagcc gggcaacatc gcggtcaatc tgcagagtgc gtcacagttc
     1081 ctcgccgacc agaagcagat tccgtcggcc gcgccgttgc agacgttcga ggccgcgatc
     1141 tacaccaagg ggttgcccgg tgccatcacc cagtgacggc accatccgga taagcaacgt
     1201 caagcaccgc tacggccgcg gccgcgacga ggtcaccgcg ctgggacccg tcgacctgac
     1261 cgtcgacccg ggatcgtttc tggtcctggt cggcgcgtcc ggctgtggca agagcacgct
     1321 gctgcggctg ctggccggct tcgagtcgcc cagcgagggc acggtcgcgg tctctggtgc
     1381 cgcgccgacg ccaggggtta cggctggcgt ggtgttccag cagccgcggt tgttcccgtg
     1441 gcgcacggtc ggcggcaacg tcgacctcgc actcaagtac gccaacgtgc ctcgcgagcg
     1501 gcgggccgaa cggcgcgatg agctgctggc gcgggtgggt ttggagggca ccgtcaagcg
     1561 caagatctgg gagatcagcg gcgggcaaca gcagcgcgtc gccatcgcgc gggcgctggc
     1621 cgccgagacg ccgctgttcc ttctcgatga gccgttcgcg gcgctcgacg cgctcacccg
     1681 cgagcggctg caggaggacg tccgtcaggt cagcgcggag tccggccgca caacggtgtt
     1741 cgtcacgcac agtgcagacg aagcggcttt tcttggttcg cggatcgtgg ttctgacgcg
     1801 acggccggga caggtggcgc tggatctgcc ggtcgacttg ccccgcacgg gcgtcgaccc
     1861 cgatgaattg cgtcgctcag ccgaatacgg tgagctgcgc gcggaagtgg ggcgctcggt
     1921 gaaggcggct gccgcttaag gcttagaccg ggggataggc cggcggtgga tacacgcccc
     1981 gcaggatcca ggcgaaccag ttgaagccgt attccagctc gtcgctcgcg tcatagtccg
     2041 ggttcggatc cacgtcatca cctctcgcgt accggctggt tctcgctgaa gtctagaccg
     2101 gacggcacag ccgcgacagt ccgaagacct aaactgtcca accagttgat tgatgcccgg
     2161 acgacgtccg ggcctgcata tagtgagtcg ccatgtccga agccaccgcc aacgggatgt
     2221 cccgccgcga cgagctgctg accgtcgcca cgaagctgtt cgccgcgcgg ggatatcacg
     2281 gcacccggat ggacgacgtg gccgacgcgg tcggcctgaa caaggcgacg gtgtatcact
     2341 actacgccag caagtcgctg attctgtacg acatctataa gggcgcagcc gacttcacgg
     2401 tcgacgctct gcacgacgac ccgacggcgt ccgcgcgcga aaccatctac cacttcaccc
     2461 gacggctact cgtcggcatc gcgggcaaca ttgaacgtgc ggccgtgtac ttccaagagg
     2521 gcccctacat caccgagtgg ttcaccgagg accaggtcgc ctacatccgc gagaaggaaa
     2581 cccaggtcta cgaacacgtg cgggacgtga tcgaccgcgg tatcgccagc ggcgagttct
     2641 acgactgcga ctcgcatgtg ctggcgctgg gctacatcgg gatgacgctg ggctcctacc
     2701 ggtggctgcg accacagggt cggcgcaccg cgcaggaaat cgccgaggag ttcagcaccg
     2761 cactcttgcg cggcctgatc cgtgacgaaa ccgtgcgcgc ggagtcgcca cttggcgtgg
     2821 acaccggtca gctgccgacg ggctgagaca acccacccgt gatggatctc gacgggttgg
     2881 tggcggaggt cgacgggcaa ctgcgcgcgc gggtggtgtc gggccggccg aacacgagaa
     2941 ggcgtacctc aagagtgcgc ttgagcacta cggggtgccg gtgccggcga tccgcgcggt
     3001 cgccaagggc gtgcgcaagc tcattcccga cctttcgcac gacgagcttg tggacgttgt
     3061 cgaggcgctc tggagcgcgc ccgtgcacga gcgccgcatg gtcgcggtgg aactgctcga
     3121 cgcttacgcc gaccgactcg tgccggccga catggtgctg ctcgaacggc tgctgcggga
     3181 atcccggacg tgggcgctgg ttgacgggct tgcggggtcg gtggtcggcc cgctcgtcga
     3241 gcgcgcgccg accgctgcgg cggtgctcga ccggtgggcg gtcgacgacg acttctggat
     3301 ccgccgctcg gcgctgttgg cgttgctcga atcactgcgc agaggcgggg gcgacttcga
     3361 acggttcggc aagtacgccg acgcgattct ggatgagaag gagttcttca ttcgcaaggc
     3421 gatcggttgg gtgctgcgcg acaccgcgcg caaacgcccg gacgtggtct ttgaatggct
     3481 gctgccgcgt gccgcgcgtg catcgggtgt gacgttgcgc gaggcgtgcg agccgctctc
     3541 cgccgaacag tgcgcggccg tcgaggcggc ccgccgcggc tgactcagaa cgcgaaccgg
     3601 ttgaccacgt cgtagttgcg catcggcgtc gcgtacatca gccggtacac caaccgctgc
     3661 ggcgtgaacg gaatcgactc gtagcccgcg accgcggaga cttgctcgac gaagcggagg
     3721 ccaaggcccc acgcctcgat cgcgcgggcg tcgcgaattc cccacttcac gattcctttt
     3781 gtggcgatct tcgacagtcg cggaccggcg ggtgagagcg tgtcgaaaag taattcacca
     3841 ctgccgaatc ggtcggtcag ccgctcgagc agctcccaga cctcgcgctc ggtgaggtac
     3901 atgaggagcc cttcggcgac gacgagcgta ggtcggccgg tcgggatctg gtccagccat
     3961 ccgggatcgg tcaccgacga gccgatcatg cgataggtgt ctgtctcgtc gtacaacttc
     4021 cgcctgagct caatgacccc gggctgatcg aggtcgaacc actgcacggt cggcggcggg
     4081 ttgattcgga acgcccgggt gtccaggccg cacccgagat gcagcaccac cgcatctgga
     4141 tggcggcgca ggaaggcggc cgcccagtcg tcgagtttct tggcgcgcaa cgccaccagg
     4201 aactgattcg cccacggctg tgctgcccga tgcactcgtt tgaagtcgta gtcgatgcgg
     4261 tcgacggctt ccgccgcggc gtgatcaccc aaaatcggca gtggcgaacg gctttcgcat
     4321 gcgcgcaggt acagcgtgac caggttcgtc cactccaccg aacgccagcc cacggagctg
     4381 aagtcgacct tggtcgtcgt catcgtttcc tcctcttggc cagcatgcca cgcgcccaga
     4441 ctgcggccag atcgccgaat tggtgatttg cgcgatctca ctgggcccac gcgaagccgg
     4501 ggacagtctc ggcgatgatg gacatgtgaa gtccaacctg ctgtcgcggc gcgacctcga
     4561 cttcctgctc tacgaatggc tgcgcatcga cgagctcacg aagcgcgagc ggttcgccga
     4621 gcactccagg gagaccttcg acggcgtcct cgatctgtgc gagcagctgg ccacccggta
     4681 cttcgcgccg cacaacaaga agagcgacgc gaacgagccg agcttcgacg gcgacaaggt
     4741 caccgtcatc ggcgaggtca aggacgcgtg ggacgcgttc gcccaggctg agttgatggc
     4801 gatgtcgatg gaccaacggc tgggaggcgc gcagctgccg gccaccatcg ccgaagccgg
     4861 attcgcctgg ttctccgcag ccaacgtgag cacgacgggc tacctcatgt tgacgatggc
     4921 caacgccaac ctgctggcca agttcggcac ggccgagcag gtcgaggcgt tcctcaagcc
     4981 gatgctcgcc ggccggttct ccggaacgat ggcgctgtcc gaaacccagg cgggctcgtc
     5041 gctggccgac atcctcaccc gcgccgaacc gcaggacgac ggtacctatc ggctgttcgg
     5101 atccaagatg tggatatccg gggccgagca cgaactcacc gagaacatcg tcaacctggt
     5161 gctggccaag atccctggcg gggcgccggg cacgaagggc atctcgctgt tcgtcgtgcc
     5221 caagtttctg gtcgacgccg acggctcggt gggtgagcgc aacgacgtga cgcttgccgg
     5281 gctgaaccac aagatgggcc agcgcggcat caccaacacg gtgctgaact tcggcgaggg
     5341 caggttcaca cccggcggaa agcccggcgc ggtcggctat ttggtcggcg aaccgcaccg
     5401 cgggctggca tacatgttca ccatgatgaa cgaggcacgg ctcggggtcg ggatgggtgc
     5461 ggtgtcgctg ggctacacgg gctacctcaa atcgctggaa tacgcgcgcg gacgcccgca
     5521 gggccggccg gtgctcgcca aggacccatc agcaccacag gtgccgatca tcgaacacgc
     5581 cgacgtgaaa cgaatgttgt tggcgcagaa ggcctatgtc gaaggcggga tggggttgct
     5641 gttgtactgc gccaagctcg tcgaccttgc gcacagcgcc gagtccgagg aggaacggga
     5701 ttcgaccggc ctgctgctcg acatcctgac gccggtcggc aagagctggc cgtcgcaatg
     5761 gtgtctggag gccaacaacc tggccatcca ggtgcacggc ggatacggct acacccgcga
     5821 atacgacgtc gagcagcact accgcgacaa ccggctcaac cccatccacg agggcaccca
     5881 cggcatccag agcctggacc tattgggtcg caaggtaacc caacgcggcg gcgccagcct
     5941 ggccgcgctc ggcgacgccg tggctgcgac ggttgccacc gccaacgccg ccggcgggga
     6001 ggcggccgag cttgccgcac agctcgacgc gacatggcag cgcctggtgg ccgtgaccac
     6061 cgcgatgttc ggctccggcg acgtcgaggc cgcgctcgcc aacagcgtgg tctatctgga
     6121 agccttcggc cacatcgtcg tcgcgtggat gtggctggaa caactgctgg ccgccaacgg
     6181 ccggaccggc gacttctacg atggcaagcg gcaggcggcg cggtacttct tcagatacga
     6241 actgcccaag acggcgccgc aactggacct gctggaaagc ctggaccgca cgaccttgga
     6301 gatgcgagac agctggtttt aatgcggtaa aggtatcgac ccatgcgata tcaggcttgg
     6361 acaggtatcg ccaacgctct ttagtttatt gagtgacggc cgccacatcg gcgcccttta
     6421 gtcagccaat acacaggagt gaacgcgcat ggtcatcacc aacgccttcc gccgccctcg
     6481 cctcagcgcc gccctcgcgg cgaccgccgc cgcgggcctg gtcctgaccg cctgcggtgg
     6541 tgtgcagacc agcaccggtg ccggcagcgg cggaggcaac tatccgaacg gcacggtcga
     6601 aatgtacgtc ggcgcctcgc ccggcggctc aagcgacctc atcagccgcg cagtatccaa
     6661 gggcctgaac gacaacctgc acgcgtcgtt ccccgtgatc aaccgcgaag gcgccaacgg
     6721 ggcgctggcc gcggccgagg tctccaaggc caaacccgac ggttcgacca tcgcgatcca
     6781 gaacgcgtcg ctgttcgcca ttacgccgct ggccgtcagc cccgatgagg tcaccaacat
     6841 cgatgatttc gacgtcgtct acggcgtctc ccgggacgac tatgtgctcg tcaccaaccc
     6901 ggcaagcggc ttcaagacgc tcaaggacct cgagaacgcc aaccgcgtgg cccgctacgg
     6961 cactacaggc gtcggcacgg gcgcgcagct cgccgccgcg ctgttgttca agtcgaccaa
     7021 cgtcgaatcg caggccgtgc cgttcgacgg cggcgcgccg gctctggccg cggtgctggg
     7081 caatcagatc gacgtggcct cgctacaagt tggcgaggcg atcgagaaca ttcagtccgg
     7141 caagctcgtt ccgctgtcgg ttttcggtcc cgagcgcatc gaatacctac ccaacgtgcc
     7201 cacggcgaaa gagcagggtc tggacgtcga ggtcgcgcag taccggttca tgacggtgcc
     7261 gaagggaact ccgaaagagg tccaggacaa gctcatcgaa ggcatgcaag ccaccttcaa
     7321 gaccgaggac tacaaggcgt tcaacaagca gaacagcctt acgcccatgg agcagccggg
     7381 cggcgaggtg ctggctcagc tgcaagcgga tcagaaacgc tatgccgaca tggtcaagca
     7441 gtacggcatc agcctgcgtg acgaaggctg accgatgaac gacggacccg cgcacacgcg
     7501 cgacatcctg gccgagatcg aggccgaggt cacccacgaa ctggaggagg agcgcccgca
     7561 gccgggtgga cccgcctatc agacagtggg cgccttgatc ggaatcgcga tcggggtcgg
     7621 cggcgcggtg ctggcctgcg gctacgggct cgggtcgctt cacgagcccg gccctggcct
     7681 gtggccgttc gtcgtgagcg ttctgatcgc cgcattgtcg atcgtcctgc tggtagttgg
     7741 tcgcggactc accgatagcg aggcattcac gcggagcagc gtgctgccgg tggtcggagt
     7801 tgccaccttc atcgcgttcg gtctgttgat gccgttgacg ggcttcgaga ttccggcgct
     7861 cgcgctctgc gtgatctggt tgcggttcct cggtggcgag agctggcgca acaccatcgt
     7921 catcagcgtg ctgacgacgg ccgtcttcta cttcctgttc ctgtacgggc tgcgcatccc
     7981 gctgcctcac ctcatcgcct tctgaaagtc atggacttca acgcatttct gaacgggttc
     8041 gtagtcgttg tcgagcccca caacctgctc tactgcctga tcggcgtgct gatcggcatg
     8101 gtgatcggcg tgctgcccgg cttgggccca gccgccacca tcgcgatcct gctgccgatc
     8161 acctacacca ttgatccggt gtcggcgatc atcatgctgg ccggcatcta ttacggcgcg
     8221 cagtacggcg ggaccgtcac ttcggtgttg ttacgcctgc ccggtgaagc gtcgtcggtt
     8281 gtcaccgtgt tcgatggctt cgccctggcc aaacagggca aggcgggaac cgcgctcggc
     8341 atcgccgcta tcggttcctt tgtaggcgcg accatttcga tcatcggcct gacgttgctc
     8401 gcgccggtgg tggcggggat cgcgctggac ttcggtccac cggagtacgc cgccctcgcg
     8461 ttgctcggtg tactgctggt cgcgacgatc ggcagtggga acaaactcaa ggcgctgatc
     8521 gccggagccg tggggcttct gctcgccacc gtggggcgtg acaacttcag cggcgcatcc
     8581 cggttcacgt tcgatagcct gcaactcgcc gacggcatcg acttcgtcgt cgtggcgatg
     8641 ggcttgttcg gcgtcgggga gattctctac aacctcgagc agcgacatcg gagggtccaa
     8701 ccgccggcca aggtgggcaa tgtctggccg tcgcgcaagg acatcaagca atcgtcaggc
     8761 gccatcggtc gcggctcatt catcgggttc gtgctcggag tgctgccggg cggtggtgcg
     8821 gtgctgtcct cgatcgtggc ctacgccatg gagaagcggc gctccaagac accggagcgg
     8881 ttcgggcgcg gcgcc
//
LOCUS       DASRJA010000657         3180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4034784,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000657 DASRJA010000000
VERSION     DASRJA010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4034784"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="VFC01_29590"
     CDS             <1..1256
                     /locus_tag="VFC01_29590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZN83803.1"
                     /translation="GSDTQLAQMARLVEDAQNGKAQAQRLADRISGVFVPIVIALSVA
                     TLGFWIGTGGSVAAAFTAAVAVLIIACPCALGLATPTALMVGTGRGAQLGILIKGPEV
                     LESTRRIDTIVLDKTGTVTTGTMTLLDVITASGEQPDEVLRLAGALEDSSEHPIATAI
                     ATAARDKLGELPSVVDFKSLEGLGVQGVVDDHKVLVGRRQLLDLPDELAKAMREAESE
                     GRTAVVVGWDGTARGVLVVADTVKPTSAEAVRRLRALGLTPIMLTGDNEAAARTIARQ
                     VGIDEVFAEVLPRDKVEAVKRLQADGRVVAMVGDGINDAPALAQADLGLAMGSGTDVA
                     IEASDLTLVRDDLRAVPDAIRLSRKTLTTIKGNLLWAFAYNVAALPLAAAGLLNPMIA
                     GAAMAFSSVFVVSNSLRLRRFTASR"
     gene            complement(1588..2145)
                     /locus_tag="VFC01_29595"
     CDS             complement(1588..2145)
                     /locus_tag="VFC01_29595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83804.1"
                     /translation="MRLDKTPIFSRVKVTAAGSINTTETTHLLGSEVIAWTSLEPSDD
                     VFAEVDVRGVLVSGSELHWMAGDAEVQMIELPHYAGMPEELIKQARPVASPSGSKAYS
                     DSPDGWPPLIWASETLHRAFKYTDYDTAVEIFLCALSVAPDDLRPMLLAGLKANHARV
                     LRHEYPQPWRTQKKPDKDMLNVLRL"
     gene            2380..2742
                     /locus_tag="VFC01_29600"
     CDS             2380..2742
                     /locus_tag="VFC01_29600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83805.1"
                     /translation="MPAGREPEIAYSGPYGEAKFQAGGRRPTQRALFALFDGGGRGDC
                     DVLSLAGGTYLGMSVTPLIERAWLVVRVARQLHRTGEVTQADVLAALRVDDGGGPGSS
                     QLASRPLRPGGCPGRSPG"
     gene            complement(2693..3180)
                     /locus_tag="VFC01_29605"
                     /pseudo
     CDS             complement(2693..3180)
                     /locus_tag="VFC01_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308603.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
BASE COUNT          536 a          975 c         1090 g          579 t
ORIGIN      
        1 ttggctccga cacgcagctg gcgcagatgg cgcgtctcgt tgaggacgcg cagaacggca
       61 aggcccaggc ccagcggttg gcggacagga tttccggcgt gttcgtgccg attgtgattg
      121 ccttgtcggt ggcaacgctg gggttctgga tcggaacggg cggatcggtg gcggcagcgt
      181 tcacggccgc agtggcggtg ctgatcatcg cgtgcccatg cgcgcttggg ctggccacgc
      241 cgaccgcgtt gatggtcggc accggcaggg gcgcccaact gggcatcctg atcaaggggc
      301 cggaagtgct cgagtccacg cgtcgcatcg acaccatcgt cttggacaag acgggcacgg
      361 tgaccaccgg cacgatgacg ctgctggacg tgatcacggc gagcggtgaa caacccgatg
      421 aggtgttgcg gttggcgggg gcgctcgagg attcgtccga gcacccgatc gccacggcga
      481 tcgcgaccgc cgcgcgggat aagctcggcg agcttccgag cgtggtcgat ttcaagagcc
      541 ttgaggggct cggcgtgcag ggcgtcgtgg atgatcacaa ggtcttggtc gggcggcggc
      601 agctgctcga tctgcccgac gaactcgcaa aggccatgcg ggaggccgag tctgaaggca
      661 ggacggcagt cgtggtcggc tgggatggca cggcacgcgg agtgcttgtc gtggccgaca
      721 cggtgaagcc gacgtctgcg gaggctgtca ggcgattgcg ggctctgggc ctgacgccga
      781 tcatgctcac cggtgacaac gaggcggccg cccggacaat cgcgagacag gtcggtatcg
      841 acgaggtgtt cgccgaggtg ctgccccgtg acaaggtgga ggccgtcaag cgactgcagg
      901 ccgatggccg ggtggtcgca atggtcggcg acggtatcaa tgacgccccc gcgctggcac
      961 aagcggatct cggcctcgca atgggcagcg gcaccgacgt cgctatcgag gcgagcgatc
     1021 tcacattggt gcgtgatgat ctgcgcgcag tccccgacgc aatccggctg tcgcgcaaga
     1081 cattgacgac gatcaagggc aacctgcttt gggcgttcgc ctacaacgtc gctgcgctgc
     1141 cccttgccgc ggcgggcctg ctcaacccga tgatcgccgg cgctgcaatg gctttcagct
     1201 ccgtgtttgt cgtcagcaac agcctaagac tgcgccgatt caccgcctcc aggtagctat
     1261 gggtcgtcgt cgtcctgcgg cctgaggtat ttctcggggt ggtggtagtc gttgactcgt
     1321 gattgtccgg tatcgaggtg tggtggtggg atccattcgc cgcaccgccg ccgacaaact
     1381 atttacacgt actcggtcgc gggctcgtcg ccccagccac gccggagcgg tcccgaggtc
     1441 gatgcgccag ccgcgcgcgt gtagcaccgc cgcctggcac ctgacgatca cgacaagcag
     1501 caacgtcgta ggcgcgccaa cacgatggcc agcagacccg agtgcagttt ccaccactgc
     1561 ctcacgcggg acagcgtaga gacggggtca tagtcgaaga acattcagca tgtctttgtc
     1621 cggctttttt tgtgtacgcc acggctgggg atactcatgg cgcaacactc tggcgtgatt
     1681 tgccttcagg cccgccagca gcatcgggcg taggtcgtcc ggtgccaccg atagagcgca
     1741 caggaagatc tcgaccgccg tgtcgtagtc ggtgtacttg aatgcgcggt gcagggtttc
     1801 agacgcccag atgagtggtg gccatccatc cgggctgtcg gaatacgctt tcgagccact
     1861 tggactcgct acggggcgtg cttgctttat gagttcttct ggcatgcccg cgtaatgcgg
     1921 caattcgatc atttgaactt cggcgtctcc cgccatccaa tgaagttcag accccgaaac
     1981 gagaacgccg cgcacatcga cttcagcgaa gacatcatca ctcggttcca ggctcgtcca
     2041 ggcaattacc tctgacccca acagatgtgt tgtctcagtt gtgttgatgg aaccggcagc
     2101 ggtgactttg acgcgggaga atatcggcgt tttatcaagg cgcatcggtg tctccttgac
     2161 tcccctcgta agacgggtgt accagacggc gggatgttaa cagggatcac ggacagcgcc
     2221 gctatcaacg aactactaac ggggctatag cttgtcggtg ggctggtaaa gacgacgcgc
     2281 cagggcgacg cgtgtgcaga cacttttgcc aggtcagcgg ctatagtcag ccctagcgca
     2341 ccggcgtttg accaccccca cgggaatgcc gagcacgaca tgcctgcggg ccgcgagccg
     2401 gagatcgcgt actccggccc atacggcgaa gccaagttcc aggccggggg tcgccgccct
     2461 acccagcgcg cgctgttcgc gctgttcgac ggcggcggtc gcggcgattg cgacgtcctc
     2521 agcctggcgg gcggcaccta cctcggcatg tcggtaacgc cgctgatcga gcgggcctgg
     2581 ctcgtggtga gggtcgcccg gcagctccac cggacgggtg aggtgaccca ggccgacgtt
     2641 ctggcggcgc tgcgcgtgga cgacggcggc gggccgggca gcagccagct cgccagccgc
     2701 cctcttcgac caggcggatg tccgggccgc agcccagggt gagctcggtg atgttggttt
     2761 ggccgtcgtt ggcccagccc tcgacgtgat ggacctgaca ccagtagccc ggcgcggtgc
     2821 agcccgggcg ggtgcagccg cgatcgagcg cgtgtaacac gatgcgctgc cccggagtgg
     2881 cgaagcgttt ggcacggccg cagtagagcg gctcgcgcgt gtgtttgtcg tagatcacca
     2941 agtagtgatg ggcctgggaa gcctgcttga tcacatcgcg catcggcaac aacgtgccgc
     3001 cacggtgacc gcaacaccgg cggcggactc gaggtcctgc agcgtggtgg acacgatgat
     3061 ggtggccggc agcccgttgt gcttgcccaa ttcccccgac gccaatgcgg cacgccccat
     3121 ggctttcagc gcgtcgtggt tgcgctgccc ctgggaccgc gtatcactgt gcacggcgtc
//
LOCUS       DASRJA010000658         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3815224,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000658 DASRJA010000000
VERSION     DASRJA010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3815224"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFC01_29610"
     CDS             complement(<1..385)
                     /locus_tag="VFC01_29610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZN83806.1"
                     /translation="MTAVRILVTGATGYVGSRLVTPLLDEGYDVVAATRNPDRLADFG
                     WDGHVSGVTLDAHDETSAKAAFANAGPIDVVYYLVHGIGQPDFRAADNRAAANVARAA
                     RDAGVRRIVYLGGFVPDDDDLSEHLT"
     gene            complement(523..1056)
                     /locus_tag="VFC01_29615"
     CDS             complement(523..1056)
                     /locus_tag="VFC01_29615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZN83807.1"
                     /translation="MSRMPEPLILPIRGRAPQLHAESWVAPTASVIGQVTLAARASVW
                     YGATLRAEAEPIEIGFGTNIQDGVTIHVDAEFPVSIGAGVSVGHNAVLHGCVIEDDAL
                     IGMGAVILNGATIGKGSLIAAGALVPQGVVIPPRSLVAGVPGSVRRDLSDAEIANNRH
                     NAQVYQRLIELHRDAVN"
     gene            complement(1081..1611)
                     /locus_tag="VFC01_29620"
     CDS             complement(1081..1611)
                     /locus_tag="VFC01_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN83808.1"
                     /translation="MPVTQDAGSALLRVLYEEHSAELWRYVLRLTRDAHQAHDVVQET
                     LLRAWRHPEITEEGTRSPRAWLFTVARNVIIDDRRTARFRNEVAMPEGADIREAVDLQ
                     DTNATIDRLMVRHALAQLSADHRAVLWRSYYLGWTLARIAEDLQIAEGTVKSRLHYAL
                     RALGRTLQQGREPDGG"
     gene            1819..>2015
                     /locus_tag="VFC01_29625"
     CDS             1819..>2015
                     /locus_tag="VFC01_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009953891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN83809.1"
                     /translation="MRIIVTGGNSGIGKATAGALAAAGHSVTIACRTIPKAEQAAAEM
                     AGNVEVCHLDLADLSSVRRFA"
BASE COUNT          356 a          662 c          644 g          353 t
ORIGIN      
        1 tggtcagatg ctcggacagg tcgtcgtcat cggggacgaa tccacccaga tacacgattc
       61 gccggacccc ggcgtccctg gccgccctcg ccacattggc agccgctcgg ttgtccgctg
      121 cccggaaatc cggttggcca atgccgtgca cgaggtagta cacgacgtcg atcgggccgg
      181 cgttggcgaa cgccgccttc gccgatgtct cgtcgtgcgc gtccaacgtg acgccgctga
      241 cgtggccgtc ccagccgaag tcggccagtc ggtcgggatt ccgggtggcg gcgacgacgt
      301 cgtacccctc gtcgagaagg ggggtgacga gccgcgagcc aacataaccg gtcgcaccgg
      361 taaccaggat tcgcacggct gtcaccgtac ggagataccc gaacccggtg aatcgaaatc
      421 ccaattatta caggaatctc atcatcgact gctcgctcag aaacctttgc gcccagaggc
      481 cttgtgcggg tgaggaattc gtggcaaaga ccagatcggg agctaattga cggcatcgcg
      541 gtgcagctcg atgagccgct gatacacctg cgcgttgtgc cggttgtttg cgatctcggc
      601 gtccgacaga tcgcggcgga cactgcccgg cacaccggcg accagcgacc gcggcggaat
      661 gacaactccc tgcgggacga gtgcgccggc cgcgatcagt gagcctttgc cgatggtcgc
      721 gccgttgagg atcaccgcgc ccatcccgat cagcgcgtcg tcttcgatga cgcagccgtg
      781 cagcaccgcg ttgtggccga cgctgacccc ggcgccaatg ctgaccggga actccgcgtc
      841 gacgtggatt gtgacgccgt cttggatgtt ggtgccgaac ccaatctcga ttggctcggc
      901 ctctgcgcgc agtgtcgcgc cgtaccaaac gctcgccctg gcggccagcg tgacctgacc
      961 gatcacactc gcggtgggcg cgacccagga ttcggcgtgc agctggggtg cacgtccccg
     1021 gatgggcagg atcagcggct caggcatgcg ggacatcgta tggatgccgc aacctcccgg
     1081 tcatccgccg tctggttctc ggccttgctg cagggtccgc cctagcgccc gcaacgcata
     1141 atgcagcctc gacttcacgg tgccttcggc gatctgaaga tcctcggcga tccgcgccag
     1201 cgtccagccc aggtaatacg agcgccagag cacggcgcgg tgatcggcgg acaactgagc
     1261 caacgcgtgc ctgaccatca gccggtcgat ggtggcgttt gtgtcctgca gatcgacggc
     1321 ctcgcggata tcggcaccct ccggcatagc tacttcgttg cggaaccgag cggtgcggcg
     1381 gtcgtcgatg atgacgttgc gcgcgacggt gaagagccag gcccgtggcg atcgggtgcc
     1441 ttcttcggtg atttcggggt gccgccatgc gcgtagcagc gtttcctgca ccacgtcgtg
     1501 cgcttggtgc gcgtcacggg tcaaccgcag gacgtagcgc cacagctcgg ccgagtgttc
     1561 ttcgtagagc acgcgcaaca gcgcactgcc cgcatcttgt gtcactggca agtgatcggg
     1621 aagtacagcc ggcacctctt caactgtcac cgtcgcagac ggtcgcgccg tccccgctgc
     1681 cacccgtcaa cgaacccccg ctaccaggta ttcgcatcgg ttcgaaatcg agtacgcaca
     1741 tactcacgtc cattgcggcg acgcggagca cactcaattc acagataaat cggtccacgc
     1801 tgaaggaacg ggaccacatt gcgcatcatc gtcaccgggg gcaacagcgg catcggtaaa
     1861 gcgaccgctg gggcgctggc cgctgcaggc cacagcgtga cgatcgcgtg ccgaaccatt
     1921 ccgaaggccg agcaggccgc cgccgagatg gcaggcaacg tcgaggtgtg tcatcttgat
     1981 ctggccgact tgtccagcgt gcgacggttc gccga
//
LOCUS       DASRJA010000659         4594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3468752,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000659 DASRJA010000000
VERSION     DASRJA010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3468752"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFC01_29630"
     CDS             complement(<1..344)
                     /locus_tag="VFC01_29630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN83810.1"
                     /translation="MTLEVVGARPAFSEEPLAGSTADRLARLPAIVEELRRHDAAAER
                     DRVLQYGAVERLRHAGLLSLRVPARYGGPGGSARDVFGVVIQVARGSSNVAQALRPHY
                     GFSERLLSNRAT"
     gene            complement(360..1376)
                     /locus_tag="VFC01_29635"
     CDS             complement(360..1376)
                     /locus_tag="VFC01_29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN83811.1"
                     /translation="MSSPRFGVWAPVYGNHGARNHPADKPDAGYRRNRDLLVRAERAG
                     FDSTLLAQHVIHPSNVEDDVLETWSTLAALAEATSRIELIGAIKPLLINPLVFAKIAA
                     NIADISGGRLAINVVSGWFLPELEALGLDAADHDDRYVYTRKWLDTVLDLWAGKQVDI
                     TGHGGHPALVRPTPDDLPQVYVGGESEPARALAAQHADVFFINGRPRSDTIDVIEDLR
                     ARPRDRAPLRFGLSAFVIARETEAEAIAELNHLQSLVDAERRPEISSGTDPKTQMYKV
                     LAGTNRVGSNGGTLAGLVGSYGQVIERIEAFHTAGVELFMLQFQPLDAELDRFADKII
                     PHFR"
     gene            complement(1373..2704)
                     /locus_tag="VFC01_29640"
     CDS             complement(1373..2704)
                     /locus_tag="VFC01_29640"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZN83812.1"
                     /translation="MTQTVDTSASERELLNGLEDGSLTEIEVAWSDPFGHAQGKRIPA
                     AQFLDRAKGHGFAFCEASLGWNTDGTVIDGLGLTNWDGGYPDVFAVPDFGTFRLLPWR
                     PGVGHVISDIVAHERNPSLLDPRAVLKRVLARLAGLGYTAKVGVELEFYLLNPDGSPF
                     QNDIHAYSLENANALDPLMTDLNETLGGFTRLEAIQTEYGPGQVETNLIYTDALEAAD
                     DAARLKYAAKEVARRHGKIASFMAKPFSEHSGSSQHLHISLWRNGTAAFASDDGTEND
                     LALHAIAGLLEHLPSITLFGAHSVNAYRRFVPDSFAPATVTWSRDNRSAAIRSLIEAD
                     PAATRIELRSGASDANPYWLVASALAAVVAGLEAGRRPPQPEGGNLYFTGTPLPESLG
                     IALALSTQDDTIIDILGTESVHDFAAIARSEWQQYSNHVSDWERQRYLATS"
     gene            complement(2701..4134)
                     /locus_tag="VFC01_29645"
     CDS             complement(2701..4134)
                     /locus_tag="VFC01_29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN83813.1"
                     /translation="MSETTTTPEALGESPAPVRQLKGNLGVAAIVFMVVAAAAPLGVI
                     GGVVPLGIASGNGAGFPATFVVSTVILLFFSIGFTALTPYVEEAGAFFSYVRKALGFP
                     IGIGIAFVALVSYVAIEAGVYGLLGPAGASIFELFHGPTLPWWAFAAVAFAVTTYLGY
                     RNIELSSRVLAVLLTAEIAIVAVLDFVIVVQGGDHGLSNGIVNASAIFSGSVGIGLLF
                     ALISYVGFEATAIFRDEARTPERTIPRATYAALILIGVFYAVTSWALISGWGDAESVQ
                     RATDSGSTFLADTAERYIGLVGTDIITVLYFTSLFACILSFHNVASRYVFALSQRDVL
                     PASLSYAHGRHGSPHLASLWISGIVAVSIVLAVAFNLDPAAQFYTWFAGATTVGFVVL
                     LIATSVAVLVFFARDRRGHSHWRVRIAPALGLVGLAAGLILIVANLKDLVGGSAVLGW
                     VIVGLLVVAFALGVVVGARVGDKATQS"
     gene            4295..>4594
                     /locus_tag="VFC01_29650"
     CDS             4295..>4594
                     /locus_tag="VFC01_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HZN83814.1"
                     /translation="MSSSVRAVGGKGKRPLRVGATPVPHAEILDFVREPLAACDIDLQ
                     IEIFESFDEPNDLLAAGRLHANFFQYLPFLDDFNRRTGNRLVPVAPVHIEPFGLYS"
BASE COUNT          826 a         1502 c         1538 g          728 t
ORIGIN      
        1 tcggtggcgc ggttactcaa cagacgttcg gagaatccgt aatgcggtcg aagtgcctgc
       61 gccacattag aactgccgcg agcgacctgg atgacgacgc cgaacacgtc gcgcgcgctg
      121 ccgccgggtc cgccgtagcg ggcgggcacg cgcaggctga gcaggccggc gtggcgaagc
      181 cgctccaccg cgccgtactg cagcacgcgg tcccgctcgg cggcagcgtc atgacggcgc
      241 aactcctcga caatcgcggg cagccgggcc agccggtctg ccgtcgagcc ggcaagcggc
      301 tcttcggaaa acgcggggcg cgccccgacc acttcaagcg tcactgttgt ctcctgcgcc
      361 tagcgaaaat gcggaatgat cttgtccgcg aaccggtcta gctccgcgtc cagaggctgg
      421 aactggagca tgaacaattc gacgcccgcc gtatggaacg cctcgatgcg ttcgatgacc
      481 tggccatagc tgccgaccag gccggccagc gtgccgccgt tggacccgac cctgttggtg
      541 cccgccagca ccttgtacat ctgcgtcttc gggtcggtcc ccgatgagat ctcgggccgt
      601 cgctcggcat cgaccagcga ctgcaaatgg ttcagttcgg cgatcgcttc ggcctcggtc
      661 tcgcgggcga tcacgaaggc ggacaagccg aaccgcaatg gggcgcggtc acgcggacgg
      721 gcgcgcaggt cctcgatgac atcgatcgtg tcgctgcgcg gccgcccgtt gatgaagaac
      781 acgtcggcgt gttgggcggc cagcgcgcgg gcgggttctg actctccccc gacatacacc
      841 tgcggcaggt cgtcgggggt cgggcggacc agcgcgggat ggccgccatg gccggtgata
      901 tccacttgct tgccggccca caggtccagc accgtgtcga gccatttccg cgtgtacacg
      961 tagcggtcgt cgtggtcggc ggcgtcgagg ccaagcgcct cgagttccgg cagaaaccac
     1021 ccgctgacca catttatggc caatcgtccg ccggagatgt cggcgatgtt ggccgcgatc
     1081 ttggcgaaca ccaacgggtt gatgagcagc ggcttgatcg cgccgatgag ctcgatgcgg
     1141 ctggtggctt cggcgagcgc ggccagcgtc gaccacgttt cgagcacatc gtcctcgaca
     1201 ttgctcggat ggatgacgtg ctgtgcgagc agggttgagt cgaagccggc ccgctcagcc
     1261 cggaccagca ggtcgcggtt gcgccggtat ccggcatccg gcttatcggc cggatggtta
     1321 cgcgcgccgt ggttcccgta gacgggcgcc cacacgccga accgtgggga gctcacgaag
     1381 tcgccagata gcgctggcgt tcccagtcgc tcacgtggtt cgagtactgc tgccactcgc
     1441 tgcgagcgat ggccgcgaag tcgtgcacgg actctgtgcc gagaatgtcg ataatcgtgt
     1501 cgtcctgggt ggagagggcg agcgcgatgc caagcgactc gggcagcggg gtgcccgtga
     1561 agtacagatt gcccccttcg ggctgcggcg gccggcgccc tgcctcgagt cccgcgacca
     1621 ccgccgcaag cgccgaagcc accaaccagt acgggttggc gtccgatgcg ccgctgcgca
     1681 gctcgatgcg ggtcgcggcc ggatcggctt cgatcagcga acggatcgcc gcgcttcggt
     1741 tgtcgcggct ccacgtcacg gtcgcgggcg cgaacgagtc agggacgaaa cgtcggtagg
     1801 cgttgaccga atgcgctccg aacagcgtga tcgacggcag atgctcgaga aggccggcga
     1861 tcgcatgcaa cgcgagatcg ttctcggtgc cgtcgtccga tgcgaacgcc gctgtgccgt
     1921 tgcgccacaa cgagatatgc aggtgctgcg agctgccgga atgttccgag aacggcttcg
     1981 ccatgaagct cgcgatcttg ccgtgcctgc gggccacttc cttggcggca tacttcagcc
     2041 gtgcggcgtc gtcggcggcc tccagcgcgt cggtgtagat gaggttggtc tccacctggc
     2101 ctgggccata ctccgtctgg atggcctcca atctggtgaa gccgcccagt gtttcattca
     2161 gatcggtcat cagtggatcg agcgcgttgg cgttctccaa cgagtatgcg tggatatcgt
     2221 tctggaacgg cgacccgtcg gggttcagca gatagaactc cagctcgacg ccgaccttgg
     2281 cggtgtagcc cagcccggcc agccgggcca atacccgttt cagcaccgcc cgcgggtcga
     2341 gcagagacgg attgcgttcg tgcgcaacga tatccgagat gacgtggccg acgccgggcc
     2401 gccacggcag aagccggaac gtgccgaagt caggcaccgc gaaaacatcg ggatagccgc
     2461 cgtcccaatt ggtcaacccc aagccatcga tgacggtgcc gtcggtgttc catcccaacg
     2521 aggcttcaca gaacgcgaac ccatggccct tggcgcggtc aagaaactgc gcggcgggga
     2581 tccgcttacc ctgcgcgtgg ccgaacggat cgctccacgc cacctcgatc tcggtcagcg
     2641 aaccgtcctc gagaccgttg agcagttccc tttcggatgc cgacgtgtcg accgtctgcg
     2701 tcatgactgc gtcgccttgt cgccgacccg ggcgccgacc acgacgccca acgcgaacgc
     2761 gactaccagg agcccgacga tgacccaacc cagcacggcc gaaccgccga ccaaatcctt
     2821 gaggttggcc acgatcaaga tgagcccggc ggccagtccc accagcccga gggccggcgc
     2881 gatgcggacc cgccagtgcg agtggccgcg ccggtcgcgg gcgaagaaca ccagcaccgc
     2941 caccgacgtg gcgatcaaca gcaccacgaa tccgactgtc gtggcccccg cgaaccaggt
     3001 gtagaactgc gcggccgggt cgaggttgaa cgcgacggcc aacacgatgc tcaccgcgac
     3061 gattccagag atccacagcg acgccagatg cggggaaccg tgcctgccat gcgcatagct
     3121 caacgacgcg ggcagcacgt cacgctgtga cagcgcgaac acatatcggg acgccacgtt
     3181 gtggaacgac aggatgcagg cgaacaggct ggtgaagtag agcaccgtga tgatgtcggt
     3241 gccgacgagg ccgatgtagc gttcggcggt gtcggcgagg aacgtcgagc ccgagtcggt
     3301 cgcccgctgc accgattccg cgtcgcccca gcccgagatc agcgcccagc tggtgacggc
     3361 gtagaacacg ccgatgagga tcagtgcggc ataggtggca cgcgggatgg tgcgctcggg
     3421 ggtgcgggcc tcgtcgcgaa agatcgccgt ggcctcgaaa ccgacgtagc tgatcagggc
     3481 gaaaagcagt ccgatgccca ctgatccgga gaagatcgcg ctggcgttga cgatgccgtt
     3541 ggacaggccg tggtcaccgc cctgcacgac gatgacgaag tcgagcacgg cgacgatcgc
     3601 gatctcggcg gtcagcagca ccgccagcac ccggctggac agctcgatgt tgcggtagcc
     3661 gaggtaggtg gtcaccgcga acgcgaccgc cgcgaacgcc caccacggca gcgtggggcc
     3721 gtggaacaac tcaaagatgc ttgcgcccgc gggcccgagt agcccataga caccggcctc
     3781 gatcgccaca tagctcacca gcgcaacgaa cgcgatgccg atgccgatcg gaaacccaag
     3841 ggccttgcgg acatacgaga agaacgcccc ggcttcctcg acgtacggcg tcagcgcggt
     3901 gaaaccgatc gagaagaaga gcaggatcac ggtggacacc acgaaggtgg ccgggaagcc
     3961 cgcgccgtta cccgaggcga taccgagcgg caccacgccg ccgatcacgc cgagtggcgc
     4021 cgccgcggcg acgaccatga agacgatggc ggccacgccg aggttgccct tcagctggcg
     4081 taccggtgcc ggtgactccc ccaaagcttc tggggtggtg gtggtttcgg acatgcgtga
     4141 ccgtcctgtc ggtggtagtt aggacggacg ataggaggaa cgccatgggc ggcaccacat
     4201 ctggagtcac cgtgagtcat atgttgtcac aattggacac attcgctgat ccgccgggct
     4261 tgcggccaaa gcatgcgtcg gtagtgttcg ggcggtgagc agctcggtgc gcgccgtcgg
     4321 cggcaagggc aagcggccgc tgcgcgtggg cgccacgccg gtgcctcacg ccgagatcct
     4381 cgacttcgtt cgcgaaccac tcgccgcgtg cgacatcgac ctgcagatcg agatcttcga
     4441 gagtttcgac gagcccaacg atctgctggc cgcgggccgg ctacacgcca actttttcca
     4501 atacctgccg ttcctcgacg atttcaaccg tcgtaccggc aaccggctgg tgccggtggc
     4561 gccggtgcac atcgagccgt tcgggctgta ctcc
//
LOCUS       DASRJA010000660         4065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3533604,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000660 DASRJA010000000
VERSION     DASRJA010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3533604"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VFC01_29655"
     CDS             complement(<1..786)
                     /locus_tag="VFC01_29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN83815.1"
                     /translation="MRAVVLKGGALHVRETADPVPGKGQLLLRPLSAAICASDVHYMD
                     HPNSAPRFVWDSDRDTIMGHEFIGEVVGHGPDCSGDFPIGSRVTSLPLLIREGAEPHV
                     IGHDPDAPGAFGELMLVSEVLARTVPDGVPNDAVAVVDAFAVGEFYVRSSAIGPGELP
                     LVIGAGAIGLSAVAALVARGVGPIIVSDYSAGRLEYAKKFGADVLVNPAERSPYEVWR
                     EVARDNGIQKPQVIFECVGAPGLLASIIGSCEFLARLFAAGGWH"
     gene            complement(788..1588)
                     /locus_tag="VFC01_29660"
     CDS             complement(788..1588)
                     /locus_tag="VFC01_29660"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN83816.1"
                     /translation="MTDFADKYGRWAVIAGASEGIGASLADQLAARGLDLVLIARNEA
                     LLNEVAARARDEHGVQTRVVVQDLTDPDAAANVAEATDGLDAGLLIYNAGASDRVTTF
                     LETEVEYSVKQIKLDCIGPISLVHHFGTAMRGRGRGGIVLVASLACLAGSATLAVYSA
                     VKAFQHNFAEGLWSELRPHGVDVCCTPLGMTYTPAFQRMGIDYDPAAHMLPADVAREI
                     IDNIGNGPVHVVGENNRAAASAVWTIDRRALVEMMSTASLDFASRTNT"
     gene            complement(1585..2025)
                     /locus_tag="VFC01_29665"
     CDS             complement(1585..2025)
                     /locus_tag="VFC01_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN83817.1"
                     /translation="MDADTEQIADVLIRYATGIDSKDWPLLRSCWTDEIDVDYQQLGH
                     FTSPDALTDVMRQLHENMGPTYHRLSNFVINVEGDQATVRSYVHAVLMLQPDDDTNWV
                     DALGHYDDVFVRTADGWRINKRVSRTARTLAGGDFATAALKGQS"
     gene            2090..2740
                     /locus_tag="VFC01_29670"
     CDS             2090..2740
                     /locus_tag="VFC01_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN83818.1"
                     /translation="MTEIARAAAADQGQRRASFQQARSHETKRSLVQAAMALWRANGY
                     ARTTVADICRAAGVSKALFYFYFPRKEDVLFEVGVLSTDAAHGVATEMLAKPYDVSGV
                     IAAVLASLENTMRSNPPELIIEAILEGYRQALAGHDHLERTSLIFAEVFERAQRDGKL
                     PPHLDVAHVANVAQSLVSEGARNWAAGAYGDRSFAEVVGRDIAALVDGYSAASAAS"
     gene            complement(2713..3204)
                     /gene="tpx"
                     /locus_tag="VFC01_29675"
     CDS             complement(2713..3204)
                     /gene="tpx"
                     /locus_tag="VFC01_29675"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HZN83819.1"
                     /translation="MAQITLRGNQINTIGELPAVGSPAPGFNLTGTDLGEVSISQFSG
                     APVVLNIFPSIDTPVCATSVRTFNERAIASGVSVLCVSKDLPFAQKRFCGAEGIESVT
                     TASAFRDGFGEDYGVTITDGPMAGLLARAIVVIGPDGNVAYTELVPEIAQEPNYDAAL
                     AAL"
     gene            3281..3727
                     /locus_tag="VFC01_29680"
     CDS             3281..3727
                     /locus_tag="VFC01_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZN83820.1"
                     /translation="MVNGVSTQQIALAPEISTWPDDNPQLIGSTCGKCGATAFPVQQR
                     CPKCSAGEMSDVLLPRRGTLVAWTTQGFPPGAPYKGPAGNDFIPFGVGLVQLGLGQDA
                     VIRVEGRLTENDPAKLQFGQQVELTMVPFTTDDEGNEIVTFAFQPV"
     gene            3749..>4065
                     /locus_tag="VFC01_29685"
     CDS             3749..>4065
                     /locus_tag="VFC01_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZN83821.1"
                     /translation="MNDVAIIGVGLHPFGRFEKTAMEMGAEAIQSALTDAGVDWKDIQ
                     FGFGGSYEVSNPDAVTRLVGLTGITFANVFNACATAATAIQQTADTIRLGKYDIGIAI
                     GMD"
BASE COUNT          725 a         1325 c         1326 g          689 t
ORIGIN      
        1 gtgccagccc cccgctgcga acagccgagc caggaattca cacgatccga tgatgctcgc
       61 aagtaggccc ggcgcgccga cgcattcgaa tatcacctgc ggcttctgaa ttccgttgtc
      121 cctggccact tcccgccaca cctcgtaggg tgagcgctcg gccggattca ccaggacgtc
      181 tgcgccgaac ttcttggcgt actccagtcg tccggcgctg tagtccgaca cgatgatggg
      241 gccgacgccg cgcgcgacca gggccgccac cgcggacagg ccgatggcac ccgcaccgat
      301 gacgagcggc agttcgcccg gtccgatggc agaactgcga acgtagaact cccccacggc
      361 gaatgcgtcg acgaccgcta cggcgtcgtt cgggacaccg tcgggcaccg tgcgcgcaag
      421 cacctccgac acgagcatga gctcgccgaa tgcccccggc gcgtcgggat cgtgtccgat
      481 gacatgcggt tcggcgccct cgcggatcag cagcggcagg ctggtgacgc gggacccgat
      541 cgggaagtcg cccgagcaat ccgggccgtg gccgacaacc tcgccgatga actcgtggcc
      601 catgatggtg tcgcggtcgg agtcccaaac gaagcgcggc gccgagttcg gatggtccat
      661 gtagtgcacg tccgacgcgc agatggcggc gctgagcggc cggagaagca gctgcccctt
      721 accgggcacc ggatcggccg tctcgcggac gtgcagcgcg ccgcctttga ggactactgc
      781 gcgcatctca ggtgttcgtc ctggacgcga aatccaatga ggcggtgctc atcatctcga
      841 ccagtgcgcg ccggtcgatg gtccagacag ccgatgcggc agcgcgattg ttctcgccga
      901 cgacgtggac cgggccgttg ccgatgttgt cgatgatctc gcgcgccaca tccgcgggaa
      961 gcatgtgcgc agcggggtcg tagtcgatgc ccatgcgctg gaaggcgggg gtgtaggtca
     1021 tgccgagcgg ggtgcagcag acgtccacac cgtgtgggcg gagctcggac cacaacccct
     1081 cggcgaagtt gtgctggaac gccttgaccg ccgaatagac cgcaagggtt gccgaacccg
     1141 cgaggcaggc cagcgacgcg acgagcacga tgccgccgcg gccacgcccc cgcatcgcgg
     1201 ttccgaagtg gtgcaccagg ctgatgggcc cgatgcagtc gagcttgatc tgcttgaccg
     1261 agtactcgac ctcggtttcg aggaaggtcg tcacccggtc tgaggcgcct gcgttgtaga
     1321 tcaacagccc ggcgtcgagg ccgtcggtag cctcggccac gttcgcagcc gcgtccggat
     1381 cggtgaggtc ctggacgacg acgcgcgtct ggactccgtg ctcgtcacga gcccgggcgg
     1441 ctacctcgtt gagcaacgcc tcattgcgag caatgagcac gagatccagt ccgcgcgcgg
     1501 ccaattggtc ggccaggctg gcgccgatgc cttcggacgc gcctgcgatc acggcccatc
     1561 ggccgtactt atccgcgaaa tcggtcatga ctgtcccttc agcgccgcgg tggcgaagtc
     1621 gccaccggcg agtgttcgtg ccgttctgga cacccgcttg ttgatgcgcc agccatcggc
     1681 ggtacgcacg aagacgtcgt cgtaatggcc aagggcgtca acccaattgg tgtcatcgtc
     1741 gggttggagc atcagtacgg cgtggacata ggagcgcacc gtcgcctgat ccccctcgac
     1801 attgatgacg aagttcgaca gccggtgata cgtcggcccc atattctcgt gcagctgcct
     1861 catcacgtcg gtgagcgcgt cggggctggt gaagtggccc agctgttggt agtcgacgtc
     1921 aatctcgtcg gtccagcagc tacgcaacag cggccagtcc ttggagtcga ttccggtggc
     1981 gtagcggatc agcacgtcgg cgatctgctc tgtgtcggca tccacacggc gaggctattt
     2041 cataagttag actcgggtca actatggatc caatgggaaa cggttgcaga tgactgagat
     2101 agcccgggcc gcggctgctg accaaggtca gcgccgggcc tcgttccagc aggcccgatc
     2161 gcatgagacg aagcggtcac tggtgcaggc ggcgatggcg ctatggcgcg cgaacgggta
     2221 tgcgcgcacg acggttgccg acatctgccg cgcggcgggc gtatcgaagg cgttgttcta
     2281 cttctatttc ccgcgcaagg aagacgtgct gttcgaggtc ggcgtgctgt ccacggatgc
     2341 ggcgcacggc gtggcaaccg agatgctggc caagccgtac gacgtatcag gcgtcatcgc
     2401 cgccgtgctc gccagtctcg agaacacgat gcgaagcaac ccacctgagc tcatcatcga
     2461 ggcgatcctc gagggctatc ggcaggccct agcaggccac gaccacttgg agaggacgtc
     2521 gctgatcttc gcggaggtgt tcgagcgggc gcagcgggac ggcaagctgc ctccacacct
     2581 cgatgtcgca catgtcgcga acgtcgcgca gtcactcgtc agcgagggcg cgcgcaactg
     2641 ggcggccggc gcctacggcg accggtcgtt cgccgaggtc gtcggtcgcg acatcgcggc
     2701 gctcgttgac ggctacagcg ccgctagcgc cgcgtcgtag ttgggctcct gagcgatctc
     2761 gggcaccagc tctgtgtacg cgacgttgcc atcgggaccg atcacgacga tggcccgcgc
     2821 gagcagcccg gccatcggtc cgtcggtgat ggtgacgccg tagtcctcgc cgaagccgtc
     2881 gcggaacgcc gatgccgtcg tcacgctttc gattccctcc gcgccgcaga atcgcttttg
     2941 cgcgaacggc agatccttcg aaacacacag aactgacaca ccgcttgcga tcgcgcgctc
     3001 gttgaacgtc cgcacgctgg tcgcacacac aggtgtatcg atcgacggga aaatgttgag
     3061 caccacggga gcgccgctga attgcgagat gctcacctca ccgagatccg tgccggtcag
     3121 gttgaaaccg ggagccggcg acccgacggc cggcagctcg ccgatggtgt tgatctgatt
     3181 tccccgcaag gttatctgtg ccatgggctc agtctgtcaa gcccggcgac agaccgcaca
     3241 gcggggccgt tctctctaga cgagaacgcc atttccgatt atcgtcaacg gcgtgtcgac
     3301 acagcagata gcgctcgcgc cggagatttc cacctggccc gacgacaatc ctcagctcat
     3361 cggaagcaca tgcggcaagt gcggagccac cgcctttccc gtccagcagc gctgcccgaa
     3421 gtgcagcgcc ggggaaatgt ctgacgtgct gctaccgcgg cgcggcaccc tcgtcgcatg
     3481 gactacccag ggctttcccc ccggcgctcc ctataagggc ccggccggca acgacttcat
     3541 cccgttcggc gtcggcctgg tgcaacttgg cctgggccaa gacgctgtga tccgtgtcga
     3601 gggccggctg accgagaacg atccggcaaa gttgcagttc ggccaacagg tcgagctgac
     3661 catggtgccg ttcaccaccg acgacgaggg caacgaaatc gtcacgttcg ccttccagcc
     3721 cgtctgagtc gagaggacac ttcgaaacat gaacgacgtc gcgatcatcg gcgtgggact
     3781 acacccgttc ggccggttcg aaaagacggc gatggagatg ggcgccgagg cgattcagtc
     3841 cgcgctcacc gacgcgggcg tcgactggaa ggacatccag ttcggcttcg gcggcagcta
     3901 tgaggtgtcc aacccggacg cggtcacccg gctcgtcggc ctgaccggga tcaccttcgc
     3961 caacgtgttc aacgcatgcg cgaccgcggc caccgctatc cagcagaccg cggacaccat
     4021 tcggttgggc aagtacgaca tcggcattgc catcggaatg gacaa
//
LOCUS       DASRJA010000661         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_566077,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000661 DASRJA010000000
VERSION     DASRJA010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566077"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFC01_29690"
     CDS             <1..274
                     /locus_tag="VFC01_29690"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HZN83822.1"
                     /translation="MDYNIFSSVNSLIGILILILIIAAVVLVVRAMVAPRAEPRSEAP
                     IVQQPVVQQPATTSGLAPGWYPDQNDPSMTRYYDGREWTSQTQPRT"
     gene            385..>2678
                     /gene="ponA2"
                     /locus_tag="VFC01_29695"
     CDS             385..>2678
                     /gene="ponA2"
                     /locus_tag="VFC01_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase/D,D-transpeptidase PonA2"
                     /protein_id="HZN83823.1"
                     /translation="MSEGRPTGVTVRRLAGCCILAGVVAAGLMFPLVGGIGLMSNRAA
                     DVVASGSAQLIAGEIPQVSTMVDTEGNPIAWLYTQRRFEVATDQIANAMKLAIVSIED
                     KRFAEHNGVDWPGTLTGLTGYLRGDLDTRGGSTIEQQYVKNYLLLVTAQTDAERRAAV
                     ALTPARKLREIRMALTLDKTFSKAEILTRYLNLVYFGNGAFGIQDAAQTYFGIDASQL
                     NWQQAAMLAGVVQSTSLLDPYTNPKGALDRRNVVLDTMIENLPQYANELRTARATPLG
                     VLPQPNGLPGGCLAAGDRGFFCDYVLDYLSRAGINKEQVARGGYLIRTTLDPKVQSSV
                     KSAIDKIASPTLDSVASVMSVIKPGKDAHRVMAMASNRTYGLNDDAHESMQPLPYSLI
                     GDGAGSIFKIFTTAAALDMGMGTDAQLQVPGSFQARGLGSSETPGCPKETWCVKNAGN
                     YRGSMNVTDALATSPNTAFAKLIQQVGVSRAVDMAVRLGLRSYALPGTARAYDPESNE
                     SLADFIKRQNIGSFTLGPFQLNALELANVAATLASGGVWCPPSPIDKIFDRNGNEVSF
                     DSQPCDQAVPEGLANTFANALSKDTSSPGTAAGAAGSVGWDLPMSGKTGTTESHRSAG
                     FVGFTNQYAAANYIFDDSNNPSGICSFPLRKCYGGDLYGGSEPARTWFEAMKPIATDF
                     GDVHLPPTDPRYVDGAPGSRVPSVTGLKVDAARQRLKEAGFQVADQATPVNSGAPYGA
                     VVGTSPSGQTIPGSIITINISNGIP"
BASE COUNT          518 a          796 c          885 g          479 t
ORIGIN      
        1 gatggactac aacattttca gttcggtgaa ctcgctgatc gggatcctga tcctcatcct
       61 catcatcgcg gcggtcgtgc tggttgttcg agctatggtt gcgccgcggg ccgagccgcg
      121 cagcgaagcg cccatcgtgc agcagcccgt cgtccaacaa ccagcgacca caagcggttt
      181 agcgccgggg tggtatccgg accagaatga cccgagtatg acgcgatact acgacggtcg
      241 ggaatggacc tcgcagaccc aacctcgaac ctgatggctt aacctgccgg caccggcgcg
      301 acaccgtacg actagggcgc cgctgcggct ccagatttcc tgtaatacac cgaattcgag
      361 cgccgccaag aataatctga atacatgtcg gaggggcggc cgaccggggt aaccgtcagg
      421 cggttggcgg ggtgctgcat cttggccgga gtcgtggcgg ctgggttgat gtttccgttg
      481 gtcggcggca tcggcttgat gtccaatcgc gccgcggacg tagtcgccag cggttcggcg
      541 caacttatcg cgggtgagat accgcaggtg tcgacgatgg tcgacaccga aggtaacccg
      601 atcgcatggc tgtacaccca gcgccggttt gaggtggcga ccgatcagat cgcgaacgcg
      661 atgaagctgg cgatcgtttc gatcgaggac aagcggttcg ccgagcacaa tggcgtggac
      721 tggccgggca cactaaccgg gctgaccggg tacttgcggg gcgatctcga tacccgcggt
      781 ggctcgacga tcgagcagca gtatgtcaag aactatctgt tgctggtgac ggcacagacc
      841 gacgccgaga ggcgggccgc cgtcgcgttg acaccggcac gcaagttgcg tgagatcagg
      901 atggcgctga cgctcgacaa gacgttcagc aaggctgaga ttctgacccg ctacctcaac
      961 ctggtgtatt tcggcaacgg cgcgttcggc atccaggacg ccgcgcagac ttacttcggc
     1021 attgacgcct cccagctgaa ctggcagcaa gcggcgatgt tggcgggggt ggtgcagtcg
     1081 acaagtctgc tcgaccctta cacgaatccc aagggtgcgt tggaccgccg aaatgtcgtg
     1141 ttggacacca tgatcgagaa tctgccccag tacgccaacg agctgcgtac cgccagggcg
     1201 acaccgttgg gtgtgctgcc ccagccgaat gggcttcccg gtggctgtct cgcggccggc
     1261 gatcggggtt tcttctgcga ctatgtgctg gattatcttt cgcgggccgg catcaacaag
     1321 gagcaagtgg ctcgcggcgg ctatctgatc cgcacgacgc tggatccgaa ggtgcagtcg
     1381 tccgtcaagt cggcgatcga caagatcgcg agcccgacgc tcgacagtgt ggccagcgtg
     1441 atgagcgtga tcaagcccgg caaggacgcg catcgcgtca tggcgatggc cagcaaccgg
     1501 acgtatggcc tcaacgacga cgcccacgag tcgatgcaac cgcttccgta ctcgctcatc
     1561 ggcgatgggg cgggctcgat cttcaagatc ttcaccacgg cggccgcact cgacatgggc
     1621 atgggcaccg acgcccaatt gcaggtgccg ggctcattcc aggcgagggg cctcggcagc
     1681 agcgagacgc caggctgtcc gaaagagacg tggtgcgtga aaaacgctgg gaactatcgc
     1741 ggctcaatga atgtcaccga tgcgctggcc acgtcgccga acaccgcgtt cgccaagttg
     1801 atacagcagg tcggggtgtc gcgcgccgtc gacatggcgg tgcggcttgg gcttcggtcc
     1861 tatgcgcttc ccggaacggc gcgtgcctac gaccccgaaa gtaatgaaag cttggctgat
     1921 ttcatcaaga gacagaacat cggctcgttc acattgggtc cgttccagct caatgcgctc
     1981 gagctggcca atgtggcggc aacgctggca tcgggcggcg tgtggtgccc gccgagtccg
     2041 atcgacaaga tattcgaccg gaatggcaac gaggtgtcgt tcgacagtca gccgtgcgac
     2101 caggcagtgc ccgagggact ggcgaacacc ttcgcgaatg cgttgagcaa ggacacgtcg
     2161 agtccgggca ccgcggctgg cgccgcagga tcggtgggct gggatctgcc gatgtcgggc
     2221 aagaccggca ccaccgagtc gcatcgatcc gcgggatttg tcggcttcac gaatcagtac
     2281 gcggcggcga actacatctt cgacgactcc aacaacccgt ccggaatctg ttcatttcca
     2341 ttgcggaagt gctacggcgg tgacctgtac ggcggtagcg agcccgcacg gacatggttc
     2401 gaggcgatga agccgatcgc gaccgatttc ggcgacgtgc atctgccgcc gacggatccg
     2461 cgctacgtcg acggtgcgcc ggggagtcgg gtgcccagcg tgacgggcct gaaggtggat
     2521 gctgcgcgcc agaggttgaa ggaggccggt tttcaggtcg ccgaccaggc gacacccgtg
     2581 aacagcggcg cgccttatgg agccgtggtc gggaccagcc cgagcggtca aacgattcct
     2641 gggtcgatca tcacgatcaa catcagcaat ggcattcc
//
LOCUS       DASRJA010000662         4158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_87165,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000662 DASRJA010000000
VERSION     DASRJA010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87165"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..333)
                     /locus_tag="VFC01_29700"
     CDS             complement(7..333)
                     /locus_tag="VFC01_29700"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZN83824.1"
                     /translation="MYTDDIEHDVVGFPGSPTRGKDGARAFYDELTANFRTEGEEVLH
                     RFATDDAVVLDQVMTGIVIGSMLGLPGNGRRISFRILHVFEFRDGLVSRENVWLDNAA
                     IVSQLS"
     gene            complement(439..966)
                     /locus_tag="VFC01_29705"
     CDS             complement(439..966)
                     /locus_tag="VFC01_29705"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN83825.1"
                     /translation="MDRPAPISTAAERILRADDGMQLRSARIAATGMDTNRKLQCALR
                     AALVDDGSMIRNGRSFVCGRPSGRRPYVVHVLPLPRTAADEIESDATALVLIVDPDKE
                     PEPAAALLRRLFGLTVGEAEVAVRLTGGASLGDIADELSVSYQTVRTHLQHIFDKTDT
                     HRQAELVRLLLTVGP"
     gene            905..1114
                     /locus_tag="VFC01_29710"
     CDS             905..1114
                     /locus_tag="VFC01_29710"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN83826.1"
                     /translation="MPSSARRMRSAAVLIGAGLSTRAIADRLTLSVRTVEGHIYRAMA
                     KTGAADRDELAAMLPSTHDSNKLIR"
     gene            complement(1021..2565)
                     /locus_tag="VFC01_29715"
     CDS             complement(1021..2565)
                     /locus_tag="VFC01_29715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZN83827.1"
                     /translation="MANRLVGFVVPLAAVAGWQALKSMGLLDYEYLPSPRELLAAVVD
                     LARSGELLIDVTHTLGVAALAAAISMAVGAALGLAIGLLRMVRRYSMASVDFLRTIPA
                     VALVPVAILSFGPVRTAEIALATYAALWPIVLHTAGGVAAVHPRQYDVARMLRLGGVT
                     TVRKIVIPAAVPAWLVGARMAVTIALLVAIVVEMLMSSHGLGGGLTESMHALAPARMW
                     AYAVVCGVLGVVLNAGLRRAVRLALPGPPAQSVGDQPTPTPPVTALRGLLPVAALLVV
                     WQLTTSDASLSFPPPDTWLSAIGRMQHDGVLAPAVLHTLGTYALGLLCAVVIGGVLGA
                     AIGSSRLIDRALTPTIDFMAAVPGAALVPVAVLLLGPGQLSGVVAVALIVSWPILLNT
                     ATAMRAIPAVRLEMSRTIGLSGPQRWGKVMVPSLIPGMMLGVRVAASLAVIITLLVDI
                     FGTGTGLGRLLVESQQRFDASAAWGLLLIVGLFGYLMSLLLSWVEGSIAANSSRSAAP
                     VLAIAR"
     gene            complement(2558..3343)
                     /locus_tag="VFC01_29720"
     CDS             complement(2558..3343)
                     /locus_tag="VFC01_29720"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN83828.1"
                     /translation="MARAVNRILYKVNSVGVRLPAADGPRQILRDVSFEVCAGEIIGI
                     VGRSGAGKTTLLRVLGGLVDSSSGTVVFDGEEVRQPPSGAVMVFQDYGNALLPWRTVA
                     RNAGLGLEGRVARAERRRRVADALSMVGLSDRGTDYPWRLSGGMAQRVQLARALTLDP
                     KVLLMDEPFGALDAITKAALQDVLLSVQQETAATVVFITHDLDEAIYLSDRVLVISGS
                     PGTITVAVDTELPRPRDQLATRELPRYLRVRHLLGQALRAVDG"
     gene            complement(3328..>4158)
                     /locus_tag="VFC01_29725"
     CDS             complement(3328..>4158)
                     /locus_tag="VFC01_29725"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN83829.1"
                     /translation="AAQAKGIFERNGLRVELTEGTDLPVFMAAMAKGQYDIVMSVPTL
                     VMIGVEKGIDLQIISSLQRSSVQRPNAVWITRDPAIESLGQLKGRTIAVPSLTGIIID
                     ATVYLLSRDGVARNDVKFVQTPFPTMGDQLAAGRVDAAVASIPFNGAIAARGFRVHDD
                     VVVDAVRDASGGTVETAMTSVWAASRSFGHEHAETVSAWRKSLSTAIELLDGNPGEAR
                     QMMQDWLRIPPRVLDGAPLPDWDVAITPQELGPYITISKAVGSTRGDPDVNALVWQGP
                     "
BASE COUNT          779 a         1454 c         1265 g          660 t
ORIGIN      
        1 ttgcggtcag ctcagttgac tgacgatcgc cgcgttgtcc agccagacgt tctcgcggct
       61 gacgagcccg tcccggaact cgaagacgtg caagatccgg aagctgatgc gacggccatt
      121 gcccggcaga cccaacatcg agccgatcac gatccccgtc atcacctgat ccaagaccac
      181 cgcgtcgtct gtcgcgaagc gatgcaacac ttcctcgcct tcggtgcgga agttcgcagt
      241 caactcgtcg tagaacgcgc gagccccgtc ctttccgcgc gtcgggcttc ccggaaatcc
      301 gacgacgtcg tgctcgatgt catcggtgta cacagcgccg caccctcgac atcgcccgca
      361 ccctccgcgc gcagatgccg agagacggcg ttgaacatct cggcactgtt catcggccac
      421 ctccccgggc ggttaactct acggtccgac cgtgagcagt agccggacga gttcggcttg
      481 tcggtgggta tccgtcttgt cgaagatgtg ttgcaggtgg gtacggactg tctgatagga
      541 caccgacagt tcatccgcga tgtcccctag gcttgcaccg ccagtcaggc gcacggcgac
      601 ttccgcttcg cccaccgtga ggccgaagag tcgtcgcaac agcgctgcgg ccggttcggg
      661 ttctttgtct ggatccacga tcagtactaa tgccgttgcg tcgctttcta tctcgtcggc
      721 cgccgtccgt ggcagcggta gcacgtgaac cacgtagggc cgccgacccg aggggcgccc
      781 acagacaaac gatctgccgt tgcgaatcat ggagccgtca tcgacgagcg cggcacgcag
      841 tgcgcactgc agtttccgat tcgtgtccat accagtcgcc gcaatgcgtg ccgagcgcaa
      901 ctgcatgccg tcgtcggcgc gaaggatgcg ttcggcagca gtgctgatcg gcgctggcct
      961 gtccactcgc gcgatcgctg atcgcctgac cctgtcggtc cgcacagtcg aagggcatat
     1021 ctaccgcgca atggctaaga caggtgcggc tgaccgcgac gaattggccg cgatgctgcc
     1081 ctccacccac gacagcaaca aactcatcag gtagccgaac agcccgacga tcagcagcag
     1141 tccccacgcc gccgacgcgt caaagcgctg ctgactctcg accagtaggc gcccgagacc
     1201 cgttcctgtg ccgaagatgt cgacgagcaa ggtgatgatc accgccagcg acgccgcgac
     1261 tcgcaccccc agcatcatcc ccggtatcag cgacggcacc atcaccttgc cccaccgctg
     1321 cggccccgac agtccgatcg tccgcgacat ctccaaccgg acggcaggaa ttgcgcgcat
     1381 ggcggtcgcg gtgttcagca ggatcggcca tgacacgatc aacgcgaccg ccaccactcc
     1441 gctgagctgg ccaggaccaa gcagcaacac cgcgacggga accagcgccg cgccaggtac
     1501 cgcggccatg aaatcgattg tgggagtgag cgcgcggtca atcagtcgcg acgagccgat
     1561 cgcggcgcca aggacgccac cgatgacgac cgcgcacagc aggccaagcg cataggtgcc
     1621 gagcgtatgc aataccgccg gcgccagcac accgtcgtgt tgcatccgac cgatggcact
     1681 gagccatgtg tccggcgggg gaaacgacag agaggcatcg gatgtcgtca gttgccagac
     1741 gaccagcagc gccgcgacag gcagcagtcc gcgcagcgcc gtaacgggcg gtgttggcgt
     1801 tggctgatct cccactgact gcgcgggcgg accgggaagg gcgaggcgaa ccgcgcgtcg
     1861 cagccccgcg ttgagcacca ctccgagaac tccgcagacc accgcgtagg cccacatccg
     1921 tgcgggtgcg agcgcgtgca tcgactcggt gaggccgccg ccaaggccgt gcgaggacat
     1981 gagcatttcg accacgatcg ctaccagcaa cgcgatggtc acggccatcc gcgcgccgac
     2041 gagccacgcc ggaaccgcgg ccgggatcac gatctttcgc actgtcgtca ctccgccgag
     2101 gcgcagcatc cgcgcgacgt cgtactgccg tggatgcact gccgccacac cgcccgcggt
     2161 gtgcagcaca atcggccaca gcgcggcgta tgtggcgagc gcgatctcgg ccgtccgaac
     2221 cggtccgaag gacaagatcg ccacaggcac cagcgccacg gctggaatcg tccgcaagaa
     2281 gtccaccgac gccatcgagt agcgccgcac catccgcagc aagccaatgg ccagcccgag
     2341 tgccgcacca accgccatgc tgatcgccgc cgccagggcc gcgacaccga gcgtgtgagt
     2401 gacgtcaatg aggagttcgc cactgcgcgc caaatccact accgccgcca agagttcgcg
     2461 cggcgacggc aggtactcgt agtcgagcaa gcccatcgac ttcagtgcct gccagcccgc
     2521 cacagcggcg agtggcacca cgaatcccac caaacggtta gccatcgacg gctctcaggg
     2581 cttggccaag gagatggcgg acccgcagat accgcggcag ctcgcgggtc gccaattggt
     2641 cacgcggacg gggcagttcg gtgtcgaccg cgacggtgat ggtgccgggg ctgccgctga
     2701 tcaccagcac gcggtcactg aggtagatcg cctcatcgag atcatgggtg atgaacacca
     2761 ccgttgccgc ggtctcctgc tggacactca gcaggacgtc ttgcaacgcg gccttggtga
     2821 tcgcgtccaa cgcaccgaac ggctcgtcca tcagcaagac cttcggatcc agcgtcagcg
     2881 ccctcgcgag ctgcacccgt tgcgccatgc cgcccgaaag ccgccacgga tagtcggtgc
     2941 cgcggtcgga gaggccgacc atgctcaacg cgtcggctac ccgcctgcga cgttcggcgc
     3001 gtgctacccg gccttcaagg cccaacccgg cattacgggc gaccgtgcgc cacggcaaca
     3061 gcgcgttgcc gtaatcctga aacaccatga ccgcgccact cggcggctgc cgtacctcct
     3121 cgccgtcgaa aaccaccgtg ccgcttgagg agtcgacaag cccgccgagc acgcgaagca
     3181 gtgtcgtttt gcccgcgccg gagcggccga cgatgccgat gatctcgccg gcgcacacct
     3241 cgaacgagac atctcgcaag atctgccgtg gcccgtcggc ggccggaagc cgaacgccaa
     3301 ccgagttgac cttgtagagg atccggttca cggcccttgc cacaccagcg cgttcacgtc
     3361 ggggtcgccg cgcgtcgaac cgacggcttt ggaaatcgtg atgtagggcc ccaattcctg
     3421 cggcgtgatt gccacgtccc agtccggcag cggagctccg tcgagaactc gtggcggaat
     3481 cctcagccag tcctgcatca tctgccgtgc ctcgcccgga tttccgtcga gcaattcaat
     3541 tgcggtgctt aatgatttgc gccatgccga taccgtctcg gcgtgctcat gaccgaagct
     3601 gcgcgacgcg gcccataccg aggtcatcgc cgtctcaaca gtgccgccgc tcgcgtcgcg
     3661 caccgcatcg accacgacat cgtcgtgcac tcgaaacccc cgagccgcaa tggcaccgtt
     3721 gaacgggatc gacgccacgg cggcatcgac gcgccccgcc gccagttggt cgcccatcgt
     3781 cggaaacggc gtctggacaa acttgacgtc gttgcgcgcc acaccgtcgc ggctcaacag
     3841 atacaccgtc gcgtcgatga tgattccggt cagcgagggc accgcgatgg tccggccctt
     3901 cagttgtccc agcgactcga tcgcggggtc cctggttatc cacaccgcgt tgggccgctg
     3961 taccgacgat cgctgcaggc tcgaaatgat ctgcaggtca atacctttct cgacaccgat
     4021 catgacgagt gtcgggacgc tcatcacgat gtcgtactgg cccttggcca ttgcggccat
     4081 gaagaccggc aggtcggtac cctcggtgag ctcgactcgc agaccgttgc gctcgaagat
     4141 gcctttcgcc tgggccgc
//
LOCUS       DASRJA010000663         8269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3470006,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000663 DASRJA010000000
VERSION     DASRJA010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3470006"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFC01_29730"
     CDS             complement(<1..232)
                     /locus_tag="VFC01_29730"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305688.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZN83830.1"
                     /translation="MTSTAHLVRAPRGPELSCKGWQQEAALRMLMNNLDPEVAEHPED
                     LVVYGGTGRAARSWEAFDAIVRTLRNLENDETM"
     gene            352..1149
                     /locus_tag="VFC01_29735"
     CDS             352..1149
                     /locus_tag="VFC01_29735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013139371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN83831.1"
                     /translation="MSSTSPAVGRALDVLLHLASRPGPVQGSALIREIGMPRSTAYHL
                     LEVLIERGFVVHLPDQRAYALGFSAFEVGSAYLRHEPLEHIARPILKRLVAAVGETAH
                     LGILYGAESVYLLKEQPQSASVPVTLVTDVGVRLPGHLTANGRSILAHLSAAQVRALF
                     PSSASFIHRTDRGPNSLAELRRILQKERAQGWAEEAGMITEGLHSVAACAFDHAGRPV
                     AAFSATRRDDRPNTTLPALVEAVRGAARQLTQALSGRPPDGWFASQG"
     gene            complement(1146..2123)
                     /locus_tag="VFC01_29740"
     CDS             complement(1146..2123)
                     /locus_tag="VFC01_29740"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HZN83832.1"
                     /translation="MQSTQQTTWPAYPPAVRGHETMHRIRVQPTDVGIAGFVDGGTLL
                     EWIERVAHATATQWCGGQCVAASVGNLHLDRPISVGEVVELQACLVYTGRSSIHILVT
                     IHSSDAGRAKVGQTAQCPIVFVALDSAGNTVEVRSWTPVTMLELQRHRQARVRIRMRK
                     RIECAMAAQSYTSDGTAPCSTLQVRTERTHALSGGRVMRWIDEAAYRCGARWAGADVI
                     TSYLAGIRFHRPIAIGDVIEVSARIIHTGPRSIHVSVHVTATDTSDGQSHLVAHGLVV
                     VVSLDERGQARPIPAWEPGSAEDHRLDLHARELIELRQFIEPFTTVRQS"
     gene            2332..2628
                     /locus_tag="VFC01_29745"
     CDS             2332..2628
                     /locus_tag="VFC01_29745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83833.1"
                     /translation="MTQLVIVAMAVGVGLVVAMLAWRARRLQRDDPEARYRKDIQALK
                     RRNWTESTRVYSDDIWSAGAVPDSPYSKSKKAAAWVALGAVGGCGGCGGCGCGG"
     gene            2616..3140
                     /locus_tag="VFC01_29750"
     CDS             2616..3140
                     /locus_tag="VFC01_29750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83834.1"
                     /translation="MRGLARRGRRDTGFERSSAAVAELELPATVFKTCPWEPRDLIKT
                     GLRQWLRCCAAALRDDQVIGMPSRAADEAWHGLILCTARYQAFCAEAYGQFMHHHPVG
                     GAPAHIEAVADPMDEQLRRTVIAWELVALPGEPCVLWDLDSRVGVEEPWGLDTARISE
                     IQDAVTKLLPSVGN"
     gene            3194..4570
                     /locus_tag="VFC01_29755"
     CDS             3194..4570
                     /locus_tag="VFC01_29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN83835.1"
                     /translation="MHTGQRQTGEAPTRSVAGNVIRGSLGNLIEWYDWYAYAAFSIYF
                     ASVFFPSGNQTAQLLNTAGIFAVGFLVRPLGGWMFGRYADRFGRRAALTLSVTLMGVG
                     SLGIAILPGYAQIGALAPILLVAARLLQGLSLGGEYGTSATYLSEVATPHRRGFYSSF
                     QYVTLTSGQLLALGVQIVLQQVLTAEQMHAWGWRIAFVVGAAAAVTVMWLRRTMDESE
                     NFALAVKESAADTERGSLRVLLCYPRECLTVVGLTLGGTIAFYTFTTYMQKFMINTAG
                     LPKEQVTRINFVALLIFVCIQPAFGALSDRIGRRPLLIAFGIGATLCTVPLLTAVSQT
                     GSALPAFGLMMIGLIIVSGYTSINAVVKAELFPARIRALGVGLPYALTVAIFGGTTEY
                     VALGLKNVGHESWFFFYVAGCGLISLLVYVFMGESSRHSQLEKETRELHRGAPEQAKV
                     DEVVADPA"
     gene            4581..5246
                     /locus_tag="VFC01_29760"
     CDS             4581..5246
                     /locus_tag="VFC01_29760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016882655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN83836.1"
                     /translation="MQVLLVEDDEGVASALAELLGQAGATVIRTARGADALHRVKGCD
                     LVLLDLGLPDMDGLDVLRTLRRASAVPVIIMTARDGDGAVVLGLRAGADDYLVKPVRK
                     AVLMARIDAVMRRIRRADGDTAAVPGPVVAGPVTIDRERREVSLAGTPQSLTRTEFQL
                     LNTLAARQGEAVSREELMLEVWGTAVVGRSRSLDFFIGQIRAKLVGLPLHTVRGFGFR
                     LDT"
     gene            5248..6630
                     /locus_tag="VFC01_29765"
     CDS             5248..6630
                     /locus_tag="VFC01_29765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN83837.1"
                     /translation="MGLRVRAVLVVLSLLAIAALAVPLALSLADRRTAALAAERDRQL
                     AALADAAAVPDAPLQRLVDRYFEVYGEGLLITDSDGRTLASRGLDITDSDVAMAVNHA
                     LVDAPASRWTRVLPWDRRHRLAADGVRNDGELVGAVVVAVDTGVAAREIATGWLWVAV
                     GCLGLLILAAGVAWSLTRWVLRPLNGLERAAAEMTEGVAGPPADVTGPPELRHFTSTF
                     NTMAQVVRASLDRQRRLIADASHQLRNPLTAVRLRADSLEDYVAEAGYSTYSSMTAEL
                     DRFENLLEQLLRLARAEQVSGSRRVGLSTAVSESTDLTDVIEERVAFWRPILDSEEQQ
                     LQYRPDDAHLSVQLARHDVEQLLDVALENALRYAGAGSTVTVSATQTGETANLLVSDD
                     GCGLPGEDLARAAARFWRGKDDGSGTGLGLAIAAEIAAGHGGAIAVERASEGGLLVRY
                     RLPIAGDSTC"
     gene            6648..7571
                     /locus_tag="VFC01_29770"
     CDS             6648..7571
                     /locus_tag="VFC01_29770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZN83838.1"
                     /translation="MSRRAFLLATAAVAAACSIRTEPGGHLRLAAGDPGGLYLAFAEI
                     LAKQLHTRYPDIPVDVLPTEGTVENLARLRSGDVDLGLALADVAERDRAGGSVGSAPQ
                     AVARVYENYLQVIVRESAVAKQLSDLQGMQVSIGAARSGGAATSDVLFEAAGLRGRVE
                     MLNYRLRDGLDRLADGSVDALVWSGGVPTPAITDLDDKLPLRILDISQLAVSMSRLAG
                     YPYAVRRVPTGGYVPPGIRSIGVPDLLLCRQHLAADLVAAVVDVLATDAPRLVPPYVQ
                     GLQYLAPPSMIQTGVIPLHWAAVGAYRKLHG"
     gene            7686..>8269
                     /locus_tag="VFC01_29775"
     CDS             7686..>8269
                     /locus_tag="VFC01_29775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83839.1"
                     /translation="MELNRESSGDRSRRGEPELVVYYPALRVRLGVDFVIVSFMFIPI
                     GALTAGSLWLLFLCANQLLARAGWGWGHLLLIQHEASWLPVAGAGLVLLPMTLLSGWF
                     MVRAAAHSLRKARRTWWLRLTSKGFQVNDRVFTPRRFEWHEIDKFMLVAPSADIGYAV
                     VGPPKTVAEAVRGGSAQSPAFRVGFDCSPGRRRKP"
BASE COUNT         1300 a         2570 c         2858 g         1541 t
ORIGIN      
        1 gcatggtctc gtcgttctcc aggttgcgca gcgtgcgcac gatcgcgtcg aaggcctccc
       61 agctgcgcgc ggcccgtccg gtgccgccgt aaacgacgag atcctcggga tgctcggcga
      121 cctcggggtc gaggttgttc atcagcatcc gcagcgcggc ttcctgttgc caacccttac
      181 aagacaactc ggggccgcgg ggggcccgga cgaggtgcgc ggtggacgtc atggcttctc
      241 ttttccctgg tcaaaactct gtggtgattc caggacaccg caccggggcc cattgcggta
      301 gtcggatttc ggggataatg tctcgtatcc gagacgcagg gaggttggca gatgtccagc
      361 acgtcaccag cagtcgggag ggccctcgac gtgctgctcc acctggcgtc ccggccgggg
      421 ccggtgcagg gctcggcgct gatccgagag atcggcatgc cgcgatcgac tgcgtatcac
      481 ctgctcgagg tgctgatcga gcgcggcttc gtcgtgcatc tacccgatca gcgggcgtat
      541 gcactggggt tctcggcatt cgaggtcggt tcggcgtatc tgcgccacga accgctcgag
      601 cacatcgccc ggccaatcct caagcggttg gtcgcggctg tcggcgagac agcccacctc
      661 ggaatccttt acggcgccga gagtgtttac ttgctgaagg agcaaccgca gtccgccagt
      721 gtccccgtca ccctggtcac cgatgtcggc gtgcgactgc ctgggcatct gacggccaac
      781 ggccgatcga tcctcgcgca tctgtcggcg gctcaggtgc gggcgctgtt tccgtcgtcg
      841 gcctcgttca ttcaccgcac cgaccgcggg cccaattcgc ttgcggagct tcggcgaatc
      901 ctgcagaaag aacgcgcgca gggatgggcc gaggaggcgg gcatgatcac tgaggggctg
      961 cactcggtcg ccgcgtgcgc cttcgaccat gccgggcgtc cggttgccgc attcagcgcg
     1021 acgcgtcgcg acgatcgacc gaataccaca ctgcccgcgt tggtggaggc cgtccgcggc
     1081 gcggcgcggc aactgaccca ggcgttgtcc ggacgcccgc cggacggctg gttcgcgtcg
     1141 caaggctaac tctgccgaac ggtcgtgaag ggctcgatga actgccgcag ctcgatcagc
     1201 tccctggcat gcagatcgag tcgatggtct tcggcggaac cgggctccca ggccggaatc
     1261 ggccgggctt ggccgcgctc gtcgagtgac acgacgacga ccagcccatg cgccacgagg
     1321 tgagactgcc cgtcgctggt gtcggtagcg gtgacgtgga cgctgacgtg aatgctgcgc
     1381 ggtcccgtgt ggatgattcg tgcggagact tcaatgacgt caccgatggc gatgggccgg
     1441 tggaagcgga tcccggcgag gtatgaggtg atgacgtcgg ctccggccca acgcgcaccg
     1501 catctgtacg ccgcctcgtc gatccatcgc atgactcggc cgcccgagag ggcatgggtg
     1561 cgttcggtac gcacctgcaa cgtcgagcac ggggcagtgc cgtctgaggt gtagctctgg
     1621 gccgccatgg cgcattcgat acgtttgcgc atccggatgc ggactcgcgc ctggcgatgt
     1681 cgctgaagtt caagcatcgt caccggtgtc cagctgcgaa cctcgacggt gttaccggcg
     1741 gagtcgagtg cgacgaaaac aattgggcac tgcgcggttt gaccgacctt cgcccggccc
     1801 gcgtcgctgg agtggatggt gacgaggatg tgaatactgc tgcgccccgt gtagacgagg
     1861 caggcctgga gttcgacgac ctcgccgacg cttatgggtc ggtcgaggtg gaggttgccg
     1921 acggacgcgg cgacgcactg gccgccacac cactgtgtgg cggtggcgtg ggcgaccctt
     1981 tcgatccatt cgagaagtgt gccgccgtcc acgaatccgg cgatgccgac gtctgtgggc
     2041 tggactcgga tgcggtgcat tgtctcgtgg ccgcgaacag ctggtggata tgccggccag
     2101 gtggtctgct gtgtcgactg catttgttgc ccccctgctg cagtagatgc agtgatcatg
     2161 agagcgaggc cacagtgcgg ggaaggggaa acggtccgat cgccgcgttt cctagcctga
     2221 tccacaggtt tcgcaaaccg gaggatgccc aatctgtgga ttgtgctagg aaacggcgca
     2281 actcggattt ttctcttccg cacggtgtgg cgcccctcac atgatcggca aatgacacaa
     2341 ctggtgatcg tcgcgatggc cgtcggggtg ggactcgtcg tggcaatgct ggcgtggcga
     2401 gcccgccgtc tgcagcggga cgacccggaa gcgcgatacc gcaaagacat tcaggcgttg
     2461 aagcgccgga attggaccga gtccacacgc gtctactcgg acgacatctg gtcggctggg
     2521 gcggttcccg actccccgta ttcgaagtcc aagaaggctg ccgcctgggt ggccctgggc
     2581 gcggtgggcg gctgcggtgg ttgtggaggt tgcggatgcg ggggctagcg aggcgtggcc
     2641 ggcgtgatac ggggttcgaa cggtcgtctg ccgcggtggc agaactggag ctgcccgcta
     2701 ccgtgttcaa gacctgcccg tgggagcccc gcgatctcat caagactggt ctgcggcaat
     2761 ggctgcgctg ctgtgcggcg gcgctgcgcg acgaccaggt gatcgggatg ccttcgcgcg
     2821 ccgcggatga agcatggcac ggcttgatcc tgtgcacggc gaggtatcag gccttttgcg
     2881 ccgaggccta tggacagttc atgcaccacc atccggtcgg cggcgccccc gcgcacatcg
     2941 aggcggtcgc cgacccgatg gacgagcagc tgcgtcgcac cgttatcgcg tgggagctgg
     3001 tcgcattgcc tggcgagccg tgtgtgctgt gggatctcga cagccgtgtc ggtgtagagg
     3061 aaccgtgggg tctcgacaca gcgcgcatca gcgaaatcca ggatgcggtc accaaactcc
     3121 ttcccagcgt ggggaattga gaatctgtgg atagtgcagg gaaatgcaac atcgagcaat
     3181 aggggtgggg actatgcata cagggcaacg acagaccggc gaagcgccga caaggtcggt
     3241 ggcaggcaac gtgatccgcg gatcgctggg caatttgatc gagtggtacg actggtacgc
     3301 ctacgcggcg ttcagcatct acttcgcaag cgtgttcttc ccctcgggga atcagactgc
     3361 gcagctgttg aacacggcag gcatcttcgc ggtcggcttc ctggtccggc cgctcggcgg
     3421 ctggatgttc ggtaggtacg ccgaccggtt cggacgccgg gcggcgttga cgctatcggt
     3481 gaccttgatg ggtgtgggtt cactgggcat cgccattctg ccgggctatg cacagatcgg
     3541 cgcgctggcg ccgatcctgc tggtcgccgc ccggctgctg cagggactgt cgcttggcgg
     3601 cgaatacggt acatctgcaa cgtatttgag cgaagtcgcc accccgcatc gacgcggctt
     3661 ctactcgtcg ttccagtacg tgaccctgac cagcggccag ctgctcgcgc tgggtgtcca
     3721 gatcgtgctg cagcaagtgc tcacagcgga gcagatgcat gcctggggct ggcggattgc
     3781 gttcgtcgtc ggagcggccg cggccgtgac cgtgatgtgg ctgcgccgca ccatggacga
     3841 gtcggagaac tttgcgctgg ccgtgaagga gagcgctgcg gacaccgagc gcggcagcct
     3901 gagggtcctg ctgtgctacc cgcgcgagtg cctgaccgtc gtgggtctca ccctcggcgg
     3961 caccatcgcg ttctacacat tcaccacata catgcagaag ttcatgatca acacggcagg
     4021 cctgccgaaa gagcaagtga cgaggatcaa cttcgtcgcc ctgctcatct tcgtctgcat
     4081 ccagccggcg tttggtgcgc tgtctgatcg gatcggtcgc cgaccgctgc tgatcgcgtt
     4141 cgggatcggc gcaacgctgt gcacggtgcc gctgctgacc gcggtgtcac agaccggctc
     4201 cgcgctgccc gcgttcggcc tgatgatgat cggactgatc atcgtgtcgg gctacacgtc
     4261 gatcaatgcg gtggtgaagg ccgagctgtt ccccgcccgg atccgggctc tcggtgtcgg
     4321 cttgccctac gcgctgacgg tcgccatctt cggcggcacc acggagtacg tcgcgctggg
     4381 gctgaagaac gtcggccacg agagttggtt tttcttctac gtcgccggct gcgggctgat
     4441 ctcgctattg gtctacgtct tcatgggcga gtcgtcccgg cactcgcagc tggagaagga
     4501 gacgcgggaa cttcacagag gcgcgccaga gcaggccaaa gtcgatgagg ttgtggccga
     4561 ccctgcttag catcagcgcc atgcaggtgc tacttgtcga ggacgacgag ggggtcgcgt
     4621 cggcgctggc cgagcttctc ggtcaggccg gcgcgacggt aatccgaacc gcccgcggcg
     4681 ccgacgccct ccatcgcgtc aagggctgcg atctggtgct gttggacctg gggctgcccg
     4741 acatggatgg cctggacgtg ctgcgcacgc ttcggcgcgc atcggcggtg ccggtgatca
     4801 tcatgaccgc ccgtgacggc gacggcgcgg tggtgttggg ccttcgcgca ggcgccgacg
     4861 actatctcgt taaaccggtc cgaaaagccg tgctgatggc gcgaattgac gccgtcatgc
     4921 gacgcatacg ccgcgccgac ggcgacaccg ccgccgtgcc cggcccggtc gtggcggggc
     4981 ccgtcaccat cgaccgcgag cgccgggagg tgtctctcgc gggcacaccg cagtcgctca
     5041 ccaggaccga gttccagctt ctcaacaccc tggccgcccg tcagggcgag gctgtcagcc
     5101 gtgaggagct gatgctcgag gtttggggca cagcggtggt cggccggtcc aggtcgttgg
     5161 acttcttcat cggacagatc agggcgaagc tcgtcggact tccgctccac acagtgcgcg
     5221 gcttcggctt ccggctggac acctgatatg ggtctgcgag ttcgcgccgt cctcgtcgtg
     5281 ctatcgctgc tggcgatcgc cgcactcgca gttcctctgg cgctcagttt ggcggaccgc
     5341 cgaaccgcag cactcgccgc ggaacgagac cggcaacttg ccgcactggc ggatgcggcc
     5401 gctgtgcccg acgccccgtt gcagcgtctg gtcgatcgct atttcgaggt gtatggcgaa
     5461 ggcctgctga tcaccgattc ggacggtcga actctggcgt cgcgcggact ggatatcacc
     5521 gatagcgacg tcgcgatggc cgtcaaccac gccctcgtcg acgcaccggc gtcgcgatgg
     5581 acccgtgtct tgccgtggga ccgacgccac aggctggccg ccgacggcgt acgcaacgat
     5641 ggggaactgg tcggcgccgt cgtcgtggcg gtcgataccg gcgtcgcagc ccgcgaaatc
     5701 gccaccggct ggctgtgggt ggcggtcggc tgtttgggcc tgctcatcct cgccgcgggg
     5761 gtcgcatggt cgctgaccag atgggtgctg cgtccgctga atgggctgga gcgcgcggcc
     5821 gccgagatga ccgaaggggt cgccgggcct cccgcagacg tgacgggtcc acccgaactg
     5881 cgccacttca cttcgacgtt caacacgatg gctcaagtgg tgcgggcctc cctggaccgt
     5941 caacggcgac tcatcgccga tgcctcgcat cagttgcgaa atccgctcac cgccgtgcga
     6001 ttgcgtgcgg acagcctcga ggactacgtc gccgaggcgg gttattcgac atacagttcg
     6061 atgaccgcgg agctggaccg cttcgagaac ctgttggagc aactgctgcg cctggcgcgc
     6121 gcggagcagg tcagcggcag ccgtcgagtc ggcctatcta ccgccgtgtc cgagtccacg
     6181 gatctgacgg atgtgatcga agaacgcgtg gcgttctggc ggccgatcct cgacagcgaa
     6241 gagcagcagt tgcagtaccg cccggatgat gcccaccttt cagtgcaact ggctcgccat
     6301 gacgtggagc agttgctcga cgtcgcgctg gagaacgcgc tgcgctacgc cggggcaggc
     6361 agcacggtga cggtgtcggc cacgcagacc ggtgagaccg ccaacctact cgtcagcgac
     6421 gacggctgcg ggctgcccgg tgaggatttg gcaagggccg cagcacgatt ctggcggggg
     6481 aaagacgatg gctcgggtac cggccttggg ctggccatcg ccgccgagat cgccgcgggc
     6541 cacggtgggg cgattgcggt ggagagagcc tcagaaggcg gcctgttggt ccgctaccgc
     6601 ctgccgatcg ccggtgactc gacgtgttga cccgtcgatc ggccgaaatg agccgtcgcg
     6661 cattccttct cgcgacggcc gcagtggccg ctgcgtgcag catccgaacc gaacccggcg
     6721 gacatctacg gttggcggcg ggagacccgg gtggcttgta tctggcattc gcggagatcc
     6781 tcgccaagca gctgcacacc cgctatcccg atatccctgt cgacgtcctg ccaaccgagg
     6841 gcacggtgga aaacctggcg cggctccgct cgggggacgt cgatctgggc ctcgcactgg
     6901 ccgatgttgc cgaacgggat cgagctggcg gttccgtggg aagcgcccct caggcggtgg
     6961 cccgggtgta cgagaattat ctgcaggtga ttgtgcgcga atccgctgtt gcgaaacaac
     7021 tctccgacct tcagggaatg caggtctcga tcggcgcagc gcgatccggc ggtgcggcga
     7081 ccagcgacgt gctgttcgag gcggccggtc ttcgcggccg cgtggagatg ttgaactatc
     7141 gcctgcggga tgggctggat cggctcgccg acggcagcgt cgacgcactg gtgtggtcgg
     7201 gcggagtgcc gacgccggcg attaccgacc tggacgacaa gctgccactt cgcatcctcg
     7261 acattagcca gctggcggta tcgatgagcc ggcttgccgg ctacccatac gccgtccgcc
     7321 gtgtgcccac cggcgggtac gtgccacccg gcatacgttc tatcggagtg cccgacctcc
     7381 tgctgtgccg ccagcatctc gctgccgacc tagtcgcggc ggtcgtggac gtactggcaa
     7441 cggacgcacc gcgtctggtg cctccctacg tccaaggtct gcagtatctc gccccgccgt
     7501 cgatgatcca gactggtgtc ataccgctgc attgggcggc tgttggcgcc taccgcaagc
     7561 tgcacggctg agcgtgaatc tgtgaatcct gcaagggttt tcgagccaag actggtagcc
     7621 gttgattgcc ggtgtggcct gccgccatga tgggcacaca acaggcaaca gttgggggca
     7681 acaatatgga actgaaccga gagtcgagtg gcgatcggtc acgccggggc gagccggaac
     7741 tggttgttta ctacccggcg ttgagggtgc gattgggcgt cgacttcgtc atcgtcagct
     7801 tcatgttcat cccgattggc gcgctcaccg ctggcagttt gtggttgcta ttcctctgtg
     7861 cgaatcaact tttggcacgc gcgggctggg gttgggggca cttgctgttg attcagcacg
     7921 aggcgtcctg gctccccgtc gccggtgccg gcctcgtctt gcttcccatg acgttgctgt
     7981 ccggttggtt tatggtgcga gccgcagcgc attcgttgag gaaggcccgg cggacgtggt
     8041 ggctgcgact gacgagcaag gggttccagg tcaacgaccg agtgttcaca ccacgccgct
     8101 ttgaatggca cgagatcgac aagttcatgc ttgtcgcgcc gtcagccgac atcgggtacg
     8161 cggtggtagg ccccccgaag acggtcgccg aggccgtgag gggcggcagc gcccagtcgc
     8221 cggcattccg cgtcggcttt gactgctcgc ccggccgtcg ccggaagcc
//
LOCUS       DASRJA010000664         7499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3753463,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000664 DASRJA010000000
VERSION     DASRJA010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3753463"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1466
                     /locus_tag="VFC01_29780"
     CDS             315..1466
                     /locus_tag="VFC01_29780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HZN83840.1"
                     /translation="MAERGLRFGLLGPLQIVVDGSALALGTPKQRAVLAALLIERNRP
                     VGIDSLANAAWQDALPGRAGASIHTYVSNLRRLLSNAGVDPRTVLVKAAPGYRLDVGD
                     DHVDLGRFTIAKTAGVHAASVGRFEEASHQLSAALNEWRGPVLQDLHDLQFFAAFAIG
                     LAEEKVVVQIARAEAEIACGREDGVIAELEVLAGEHPYREPLWAQLITAYYLAGRQSD
                     ALDTYRRLKSTLADDLGIDPAAPLRTLHQRILRQESLDVRRAAQTTAEKTIVATGGRT
                     PAHAGSAVARLRDASGQCYVLRAAATRIGRRADNDIVLADPKVSRYHAVIIDTGTSYL
                     ITDLSANGLEVQQQRVRTSATLADGDQIRIGLNNFTFEVSAEAAPSAIS"
     gene            1683..1985
                     /locus_tag="VFC01_29785"
     CDS             1683..1985
                     /locus_tag="VFC01_29785"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN83841.1"
                     /translation="MKFKKAIAGSVLGLALGIGVATAGAATAQADEGSYIDDLSSAGF
                     YGDSGEALAVGYAVCDLVGEGYSQDELFDAVYVTTDESITASDAQYIVESAEIFLC"
     gene            2117..3664
                     /locus_tag="VFC01_29790"
     CDS             2117..3664
                     /locus_tag="VFC01_29790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014984963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83842.1"
                     /translation="MNGDLTPALIETDVWTDDMVAAAGIPIVDVPFVTIGGGIGSFVT
                     FDTLRIYGVPAAAIASLGPQAVPWSSYEHLVRVSQIPRRERIRSDSASTPDNIWGFPS
                     YAVREAFGARSLSGFIKPLWNVAVEPIFANYYTPRAGQVFESLEREHQRVGYAQSVRI
                     GDVRMVRRRSGGGYFTILTPPPGSSPTKRVAFRSTYAHIAVGYPGLNMLPELQEFRER
                     YRDPSKVVNAYESHEHIYQSLRRRPGTVMIRGSGIVASRVLQRLIDDRDKYRLRTTIL
                     HLFRTYVAEPHGSSIFKRRKGKDGWAYQGFNYPKSVWGGQLKAKMRKLEGEDRKQAYK
                     EMGGTNTPVRKNWQEQLRRGRTEGWYHVMAGTARDLRPTRDGLTTTVVPDPSQPLPYP
                     PPTHPFDVTVNYVIDCTGLEADIREHRLLADLLDHTGAGRNPLGRLDVDRTFELIGTR
                     SGTGRIYASGTATLGGYFPGVDTFLGLQIAAREIADDLAAHGFCRRMGPMRSVHQWWL
                     WARNQRI"
     gene            3671..4114
                     /locus_tag="VFC01_29795"
     CDS             3671..4114
                     /locus_tag="VFC01_29795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006867360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83843.1"
                     /translation="MLPTLNGRIQTRVLGLGIIGFFVALVITPVLPTGDLSLTQSYRV
                     TLSVLLAVVLVGVPWELLYHGLQQFRWEKDWPTLFGLLTAVSEGIVVWLLVRYTTIVV
                     PEDLRPSLTAYLIQFIITWLAFWLVVNGPMRVLFHRWRFAGGRFI"
     gene            4111..5277
                     /locus_tag="VFC01_29800"
     CDS             4111..5277
                     /locus_tag="VFC01_29800"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZN83844.1"
                     /translation="MNSPQIGVIPGGHLVAVANGTVVVLPHRERSALSSESQAAQTWS
                     ALYALIVEAAARDGDRYGRSFAENAAKWAADRGGHAEFGALTPVQGGYALILHGGVAA
                     IIEHEGGRDHYRGSRGCPTEVSEIGSVANRAALYVTDDALPTIDLPAERGICSLIEGV
                     VEGAGAVLWLTGTKPAARPVAKAAHHVELTPPKVFETFKPNDKAPPRRAPLPIKSIPH
                     SGAATPNRPPVGVGSRPVHGIKCARGHFNDPRVAFCRLCGLRMNQTKALYEGERPPLG
                     FLVLDDGTTLVLAADLVIGRDPRNSPSVRTGATPVPLSDAAGRLSRAHAEIRLIEWDV
                     TVVDLASKNGTFVKPPGHEQWLRLTPRRPHLLTAGAEVQIGGRTITFESAHAHL"
     gene            complement(6146..7381)
                     /locus_tag="VFC01_29805"
                     /pseudo
     CDS             complement(6146..7381)
                     /locus_tag="VFC01_29805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000691.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
BASE COUNT         1429 a         2460 c         2294 g         1316 t
ORIGIN      
        1 cgtgccgccg tacgtgccgc accaggagat caacgccagt cctgacgcgc ctttgtcgtg
       61 cgtggtggtg cgtagcgatc aggaaccggt ggtcgtcaat ctggatttac ccgacatcga
      121 acccgctccc gagcacgtgc aatgggtcga cgacatacac ccgccgcaat aagcctcgcc
      181 acccgcacac cgtcgccgat ctcgctggat tcggtgtcta caccgacccc accggccgat
      241 aatcggccga cagaagtgca acaactgcgg gcaagttagg agagcggttc aacctcaggg
      301 agggtaacga aagaatggcg gagaggggtc tcaggtttgg cctcctggga ccgctacaga
      361 tcgttgtcga cggcagcgct ttggcgttgg gaacccccaa gcaacgcgcg gtcctggcgg
      421 cgctacttat cgagcgcaat cggccagtcg gtatcgattc gctggccaac gccgcgtggc
      481 aggatgcgct tcctggacgg gcgggtgcca gcatccacac ctacgtctcg aacctgcgga
      541 gactgctgag caacgcaggc gtcgacccga gaacggtgtt ggtcaaagca gcgccgggtt
      601 atcggctcga cgtcggcgac gatcacgtcg atctcgggcg attcaccatc gcgaaaactg
      661 cgggagtgca tgccgcgtcc gtcggccggt tcgaagaggc aagccaccaa ctgtcagcag
      721 cgctaaatga atggcgcgga cccgttctgc aagatctgca cgaccttcag ttcttcgcag
      781 cgttcgccat cggactcgcc gaggagaagg ttgtcgtcca aatcgcccgc gctgaagctg
      841 aaatagcgtg cggtcgagag gacggggtca tcgccgagct ggaagtcctc gccggcgagc
      901 acccctatcg cgagccttta tgggcgcagt tgatcactgc ctactatctc gccggacgtc
      961 aatccgacgc actcgacacc tatcgccgat tgaaatcgac cctcgccgac gatctcggca
     1021 tcgaccccgc cgccccgcta cgaaccttgc accagcgcat tctgcgccaa gagtcgctgg
     1081 acgtacggcg ggcagcgcag acgaccgccg aaaagacaat cgtcgctacc ggcggacgca
     1141 caccggccca cgccgggtcg gcggtcgccc ggctgcgcga cgcttcggga cagtgctatg
     1201 tgttgcgggc ggcggccacc cgaatcggcc gccgcgccga caatgacatt gttctcgccg
     1261 acccgaaagt cagccgctac cacgcagtca tcatcgacac cggcaccagc tatctcatca
     1321 ctgacctatc cgccaacggg ctcgaggttc aacaacagcg tgtccgaacc tccgccacac
     1381 tcgcagatgg cgatcagatc cgaatcggct tgaacaactt cacatttgaa gtctccgctg
     1441 aggctgcacc ctcggccata tcgtgatacc gagttggtcc tagatcgaca tcgcggacca
     1501 atcactctcg tcaacaacgg atctcgactg gcacagaacc gtctgacccg ccttcgcgcc
     1561 catctacatg aacacgtttg agagagctgc tgcggctttg aggatctttt gaggccgccg
     1621 attcattctc acttccacca aaggacggcg cagtgccatc caccaacaga aaggaagcga
     1681 aaatgaaatt caagaaggcg atcgcaggat ctgtgctcgg gctggctctc ggtatcggtg
     1741 tagcaaccgc aggagcggcc accgctcaag ctgacgaagg cagctatatc gacgacctgt
     1801 ccagcgctgg gttctacggc gacagcggcg aagcacttgc agtcggctac gcggtctgcg
     1861 acctcgttgg cgaagggtac agccaggacg aactattcga cgcggtctat gtcaccacgg
     1921 acgagtcgat caccgcgtcg gatgcccagt acatcgtgga gtcggcggag attttcctct
     1981 gctgaccaga cacctcgggc gatgcgcccc cggctacggg cgggggcgct gcccccgttc
     2041 taatcggcgg ccgccaatcc gctcttaacg cataagcggt gcacccatac tgattcagca
     2101 cgcctaggga gcaaacatga atggagatct gacaccggcg ttgatcgaga cagatgtctg
     2161 gaccgacgac atggtggcgg ctgccgggat cccgatcgta gacgtgccct ttgtcaccat
     2221 cggcggcggc attggctcgt tcgtaacgtt cgacacactg cgcatatacg gggtgccggc
     2281 ggcggcgata gcgtcgctgg gtccccaggc ggtcccgtgg tcgagctacg aacacctcgt
     2341 ccgcgtgtct cagattccgc gtcgcgaacg gatccgctcg gattcggcct cgacgcccga
     2401 caacatctgg ggattcccgt cgtacgccgt tcgggaggcg ttcggtgccc gatcgttgtc
     2461 gggattcatc aaacccctct ggaacgtagc ggtcgaaccg atcttcgcga actactacac
     2521 gccgcgcgcc gggcaggtat tcgaatcgct ggaacgcgaa caccagcgtg tgggttacgc
     2581 gcagtcggtg cgcatcggcg acgtgcggat ggtccggagg cgaagtggcg gcggctactt
     2641 caccatcctc accccaccgc cgggatcgtc accgacgaaa agggtcgcgt tccgcagcac
     2701 gtacgcacac attgcggtgg gctaccccgg cttgaatatg ctgcccgagc tgcaggaatt
     2761 ccgcgagagg taccgcgatc cgtcgaaggt cgtcaacgcc tacgagtccc acgagcacat
     2821 ctaccagtcg ttgcggcgtc gcccggggac ggtgatgatc cgcggcagcg gcatcgtcgc
     2881 gtcgcgggtc ttacagcgcc tgatcgacga ccgcgacaag taccggctac ggaccacgat
     2941 cctgcacctg ttccgcacct acgtcgccga gcctcacggg tccagcatct tcaagcgccg
     3001 aaaaggcaag gacggctggg cataccaagg attcaattat ccgaagtcgg tctggggagg
     3061 ccaactcaag gcgaagatgc gcaagctcga gggcgaggac cgcaagcagg catacaaaga
     3121 aatgggcggg accaatacgc cggtccgcaa gaactggcag gaacagctac ggcggggccg
     3181 caccgagggc tggtatcacg tgatggccgg gacggcgcga gacctgcgtc cgacccgcga
     3241 tggtctgacg accacggtcg ttcccgaccc cagccagccc ctgccgtacc cgcctccgac
     3301 gcacccgttc gacgtcacag tgaactatgt catcgactgc accggactgg aggccgacat
     3361 ccgggagcat cgactgctcg ccgatctgct cgaccacacc ggtgcgggcc gtaatccgct
     3421 cggccggctc gatgtcgacc gcacgtttga gttgatcggt acccgcagcg gcaccggacg
     3481 gatctacgcg tcaggcaccg ccacactcgg cggctacttc ccgggggtgg ataccttcct
     3541 cggcctgcag atcgcggccc gggagatcgc cgacgatctc gccgcgcacg gtttctgccg
     3601 ccggatggga ccgatgcggt cggttcacca atggtggttg tgggccagaa accagaggat
     3661 ctgaggtcgc atgcttccga cgctgaacgg gcgaatacaa acccgtgtgc ttgggctggg
     3721 catcatcgga ttctttgtcg cgcttgtgat taccccggta ttgccaaccg gcgacctgtc
     3781 actgacgcaa agctatcggg tgacacttag cgtcctgctc gcggtcgtcc tggtaggagt
     3841 gccgtgggag ctgctgtatc acggtttgca gcagtttcgg tgggagaagg attggccaac
     3901 cttgttcggg ctgctcaccg cggtcagcga gggcatcgtg gtttggttgc ttgtccgcta
     3961 caccacgatc gtggttccgg aagatttacg accgtcattg acggcctact tgatccaatt
     4021 catcattacg tggttagcgt tctggctcgt cgtcaacggt ccgatgcggg tgctatttca
     4081 ccgttggcgt tttgcgggag ggcggttcat atgaacagtc cccaaatcgg ggtgatcccc
     4141 ggtggtcacc ttgtggcggt cgcgaacggc acggtggtcg tcctgccgca ccgcgagcgg
     4201 tccgcgctga gtagcgagtc gcaggccgca cagacatggt cggcgctcta tgcgctgatc
     4261 gtggaagctg cggcaagaga cggcgatcgg tacgggcgct cgttcgcgga aaatgccgcc
     4321 aagtgggcgg ccgatcgagg cggtcacgcg gagttcggtg cgctgacacc cgtccagggc
     4381 gggtatgcgc tgatcctgca cggcggcgtt gcggccatta tcgagcacga gggtgggcgg
     4441 gaccactatc gcggcagccg cggctgcccg actgaagtct ccgaaatcgg ttcggttgcg
     4501 aaccgagccg cgctgtacgt cacagacgac gccttgccaa cgatcgactt gccggccgaa
     4561 cgtggaatct gctcgctcat agagggtgtc gtcgaaggcg ccggtgccgt tctgtggctg
     4621 acaggaacaa aacccgctgc ccggcccgtc gcgaaagcgg cccatcatgt cgagctcacg
     4681 ccaccaaagg tcttcgaaac gttcaagccc aacgacaagg caccaccgcg gcgcgctccc
     4741 ctacccatca aaagcattcc gcattccggg gcggctactc caaaccgccc acctgtcgga
     4801 gtcggcagcc gtccagttca cggaatcaaa tgcgcgcgtg gacatttcaa cgatccgcgg
     4861 gtcgcgttct gccgcctatg cggactgcga atgaaccaga ccaaggctct ctacgaaggg
     4921 gaacggcccc cacttggatt cttggtcctc gacgacggca cgacgctcgt cctggctgcc
     4981 gatctggtca tcgggcgtga cccgcgaaac tctccatcgg tccgcactgg agccacgccg
     5041 gttcctttat ccgatgcggc gggtcgactg tcgcgggcgc atgccgagat tcggctcatc
     5101 gaatgggacg tcacagtcgt cgacctcgct tcgaaaaacg gcacgttcgt gaagccgccg
     5161 ggacacgaac aatggcttcg gctgacgccg cggcgaccgc atctgctcac cgcgggagct
     5221 gaggtgcaaa tcggcggtcg cacaattact ttcgagtcgg cacacgccca cctgtaacga
     5281 ccgatcgtcg gtattagcga agcttcactc gcagcaccgc gttgttcggt tcgtcgctca
     5341 gccacagtgt gtcgtctagc aacacaatgc ccacgattcc cttgccgatt cccgtgaccg
     5401 cgctcccctt agcctcctgg cccttgaggt ccagccattt cagctcgttg gacaccggaa
     5461 acagcaccca cagaccgtta gtgcccgccg aaaagtagct cccacccttg agcggtatcg
     5521 gctcgcccac cgcgaaggat ccctcgtcga tcggggtgac gtcgccggtg gatccaacgt
     5581 agatggtgcc atcaacgacc tctatgccac ccagctcgtc gttgagcttc aagggctggc
     5641 cgagaacggt gcccgtgcta tcgatcgtgg tgatggtgct gtcgcctgaa ttgaccacgt
     5701 agagaaagtg ctcgccgaaa ctcatcgctg cgggggctgc cccaacctgg atcggcgcgc
     5761 cgatgagcgc ctgggtttgc gtgtcgatcc gcgtcacggt gttctcggcg gtgtgtgacg
     5821 cccacaggta gccgccacca ccggcgatcg ccgcgatgtc tcctggcccg gcagctatcg
     5881 ggtcgctgac actttcggtc gccaggtcca cgcgagtgac tgcgtcggtg tagttccaca
     5941 cccagaccgc gtcctgggtg acgaccagtt cgtttggtac gccgccgacc tcgatctggt
     6001 tggcggttcc cgtcgacggg tcgatcttgg agatcgtggc gtcattattc gcggtccaga
     6061 cgaacccgtc gccgacttcg atgtcgtagg gttccttgcc taccaggatg ggatcgcctt
     6121 ccaacacgcc gatgggctgg ggcccctcgg ggccacggtc acgcaaagcg aaccagcccc
     6181 cgacgccggc gaccgccagc accagcacgg ccaccagcgc aacgatcagc ccggtgcgcc
     6241 gccgccgggg tgggctgaca atcggcaacg gttgcgaggg cggctgctgc agacctgccg
     6301 gcggatgcgg cggcccactg ggcgtccaca cccccggtgg tgggacgggc ccggccgcag
     6361 gcggggggtt ggcataaagg gtcggcgccg acaacaccgg ccccggcgtc ggcgcgggac
     6421 gcggcggcgg cgtgaccgga cgcggcggcg gttgcgccaa cagcgtggac ggctggccgg
     6481 ccaccgtcga gggcggaagg gtcgccaact gactccggtg cagaatatct gcggcccgat
     6541 cctgttcggg tgtggcaaga gcttcatggg cagccaacgc cagatccccc gccgccgcat
     6601 agcgatcctc cggatccttg gccataccac gcgcaaccac ctgatcgaag gccaccgaga
     6661 gccccggccg aagcgcactg ggccgcggga tggactgggt cagatgcgcg gtcatcaacg
     6721 tgacgtcatt gccgcgatac ggcggagctc cggtcaagca ctcgaagagc acacacgcca
     6781 aggcataaat gtcggcgcgg taagtcacct gcgactcggt gaaccgctcc ggagccatat
     6841 acctaaacgt tccgatggca ctgcccatct gcgtcagctt ttcgtcggtc gtggcactgg
     6901 caataccgaa atcgaccaga taagcaaagt catcatgagt gatcaggatg ttttccggtt
     6961 tcacatcacg atgcatcacc ccggcggcgt gcgcagcatc gagcgccgag gcgatctggc
     7021 ggataatcgc caccgtccgc ggcggcgcca gcggcccata tcggctcaac atcgtcgcca
     7081 cgtccatgcc ctcgatcagg cgcatatcca catacagctg cccgtcgatc tcgccgaagt
     7141 cgtggatggg caccacatgc ggttcctgca accggcccgc cgtgcgggct tcgcgctgca
     7201 tccgcttgcg aaacaccgga tcccccgaga acgccgccga catcaacttc aacgccacca
     7261 cacgttcctt gacggtgtcc tcggcctcat acacatcacc catgccaccg cggcccaaca
     7321 gccgacgcag gtgatagtgc ccaaaccgcg aacccgcccg cgaatcctcg gtgtcgctca
     7381 tcgccgactc ctcaggtggt tagccagctc taacgctaaa tcctgcccag gcccaccgca
     7441 gcaaaaccgc actcacacaa catcggccac cgcatcacgc aaattgacct gcgaaccac
//
LOCUS       DASRJA010000665         8011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3389213,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000665 DASRJA010000000
VERSION     DASRJA010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3389213"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFC01_29810"
     CDS             complement(<1..698)
                     /locus_tag="VFC01_29810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HZN83845.1"
                     /translation="MADRTAGLRSAAAHAEAFLATLDERPVAARADAAAIRDALGGPL
                     PDHGDDPAAVIDGLAAGAEPGLVATAGPRHFGFVIGGALPAALAADWLVSAWDQNAAF
                     HSLSPAAAAIEETTSAWTLELLGLPEGASVGFVTGAQGAITTCLAAARHAVFARARWD
                     VERDGLIGAPPVRILCGEQAHATIYTSLRLLGLGAETAIRIPVDDQGRMDPDALAKAL
                     AGNNSPTIVCAQAG"
     gene            complement(713..1903)
                     /locus_tag="VFC01_29815"
     CDS             complement(713..1903)
                     /locus_tag="VFC01_29815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZN83846.1"
                     /translation="MVLGGDPGRVPSDVTLENWQSAAQLHWTFQHIAEFLPTAVISRG
                     TGPVANLPSAPSELSDIPLLDRTNGKRTTVGDVMAATVTDGWIVTQHGKVVAEHYYSG
                     MTADTSHLLMSVSKSLIAMVAGALVSDGALNVDAELTTYVPVLANSGYAGSTVRHLLD
                     MRSGIAFSEDYLNPLADVRLLEQAIGWAPRTVPDLPTTMYDFLLTLKQKAPHGGPFQY
                     RSCETDVLGWICEAVSGIRMPELMSQLLWSKLGADNDATIGVDSVGTGMFDGGINACL
                     RDLARFGSLFLNGGVSLTGERVVSLSWIADTFSGGVDSRAAFAASPDDNRMPGGMYRN
                     QFWFPYPGNNVLLCLGIHGQMIYVNRAANLVAAKLSSWPLPQDATKLFPTIAAFDEIA
                     ATLS"
     gene            complement(1949..3625)
                     /locus_tag="VFC01_29820"
     CDS             complement(1949..3625)
                     /locus_tag="VFC01_29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HZN83847.1"
                     /translation="MSDRPVRARRPSTATKKATKKDTAKKSAGRSTPAKRPTKAAAAK
                     TTRRARATAKPREVSIGTDAAFASGSPPRRRLKNISEVRHFFRTNDVPILFFGATPFN
                     LLGLDRWVRNFSYITYYDAWDGAHPRVFTPAHKPYREFESGEEINNWLLTSAEVRAHI
                     DAATPRGVRPKVAMVFFNSETEDICKELGYDLILPAASLRQHLDSKIVTTRLGNEAGA
                     PSVPNVLTRVDDWAGLQAVAQKARLGDELVVQTPYGDSGKTTFFVSSEADWRKHSADI
                     VGEEIKVMRRINNRPVAVEAVLTRCGTVVGPFMSELIGYKRLTPSRGGWCGNEMFPEV
                     LTGESRRTATQLVRRLGDRLAKEGYRGFFEVDVLVDTDTNDVYLGELNPRISGASSIT
                     NVTAGAYADVPLFLFHILEYMNVDFDLDVDEINERWEELASADVWSQMVMKETDDIVQ
                     RLTATPRTGQYSLDASGGLVFRRSALDWHQLQNEREAFFLRIYGPGDYRWKGADLGVL
                     VTKGRLQVDSGQGKSSLAIRARHLIDSIRAEYAGEPIAEPATARTDVGVK"
     gene            4015..4533
                     /locus_tag="VFC01_29825"
     CDS             4015..4533
                     /locus_tag="VFC01_29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN83848.1"
                     /translation="MDKQSADHHAGDCVGELLDQAMDLTARFLSDRADLSASAAFAMN
                     RVCREGPIRLTALAAKEGVSQPSMTQLIQRLERLDLVARLADPDDGRAALIGITERGQ
                     ALLNDRKRMRRQRLKALMATLTPEEESALLLSARVALPVLRRLVENADCPADGEAAQQ
                     ATTETAGRKVLG"
     gene            4530..4970
                     /locus_tag="VFC01_29830"
     CDS             4530..4970
                     /locus_tag="VFC01_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HZN83849.1"
                     /translation="MKAVPVGRALKRLWIPVLLVVVLAVSGLVVSRLHKMFASQDLNA
                     NAGAGIEIVQFNPKVMVYDVYGSPGTTAQISYFDTDANVHSITAPLPWSVTLSTTLPT
                     VSASLMAQTDGDQIGCRVTVNGTVREEQSANGVNAQTYCLVKSA"
     gene            4967..7861
                     /locus_tag="VFC01_29835"
     CDS             4967..7861
                     /locus_tag="VFC01_29835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN83850.1"
                     /translation="MTTTTTAAGHEKRPVIAHSLRILAVPIILFWIAVAVLVNVLAPQ
                     LEVVGELHSAPMAPEDAPSMVAMKLMGANFKEFDSNSTIMIVVEGQKPLGPDAHAYYD
                     EIIRKLRQDPKHIQHIQDFWGDTLTAAGAQSADGKASYVMLNLAGEQGQTLANEGVES
                     VRKVIEETKAPPGVQAYVAGPAALTDDLHVIGNASLAQITLFTLIAIAGMLLVVYRSI
                     RTTLIQLFLTFIGLLTARGVISVLASHDAFGLTTFAGNILTMLAIAAATDYGIFIFGR
                     YREARASGLDRDDAYYTTFKSVAPVIVGSGLTIAGATYCLSFARLPYFSTMGAPVAIG
                     MVVVVASAVTLGPAVLFLGSRVGLFEWKRPPKGRFWRRVGTIVVRWPAPVLVASAFVV
                     LIGLVAVPGYKPAYNDRYYLPADAPTNIGFAAADRHFTQARMNPDILMVEADHDMRNP
                     ADMLVLNKISSNVMHTEGIAMVQNITRPLGIPIQHSSIPFQTSVQGQTNNMNLPFQRD
                     QLANQLKTIDETNRSIDILEQQYQLSLEQTKLTQDSAAKSQALLQTTEELRDNIANFD
                     DQFRPLRNYFYWEPHCFDIPLCNATRNLFDALDGIDRVTDQTGAVQVNTDQLADLAPK
                     LTALLPQTIASMKASRDLSLASYNSQKALLDQLEASNNTALAMGAAFDGAKNDDLFFL
                     PPEAFDNPDFKRGLVMFLSPDGKSARMFITHESDPATVDGIKRVEGERKAAQEAMKMS
                     SLSNAKIYLGGVAATYKDMADGARYDLLIAVVSSLTLIFMIMLILTRSAVAAFVIVGT
                     AASSIAASFGISVLLWQDLFGIQVQWLVMLMSVIILLAVGSDYNLLLVSRFKDEIHAG
                     LKTGIIRSMAGTGGVVTSAGLVFAATMAAMMGSKLVVLAQMGSTIAIGLLVDTFVVRS
                     FLMPSIATLLGRWFWWPQVVYPRGDYHFLPPQPRRRATDDPDTVALPAQA"
BASE COUNT         1406 a         2549 c         2605 g         1451 t
ORIGIN      
        1 ttgccggctt gtgcacacac gatcgtgggt gagttgttgc cggccaacgc ttttgcgagc
       61 gcatcggggt ccatgcggcc ttggtcgtcg acgggtatcc ggatcgcggt ctccgcacca
      121 agccccagca gccgcagcga cgtgtagatg gtggcgtgcg cctgttcgcc acacaggatg
      181 cgcaccggcg gtgcgccaat cagcccgtcg cgctccacat cccagcgcgc acgtgcgaac
      241 acggcgtggc gtgcggccgc caaacacgtg gtgatggcgc cctgggcacc ggtgacaaag
      301 ccgacgctcg cgccctccgg caatccgagc aactcaagcg tccacgctga cgtggtctct
      361 tcgatggcgg ccgcggcagg ggagagcgaa tgaaaggcag cattctggtc ccacgccgac
      421 accagccagt cggcggccag cgcggcgggc aacgcaccgc cgatgacgaa tccgaagtgg
      481 cgggggccgg cggtcgcgac gaggcccggt tcggcgcctg ccgcgagccc gtcgatcacc
      541 gcggcgggat cgtcaccatg atcgggcagc gggccgccga gcgcgtcacg gatggccgcg
      601 gcatcggcgc gggcagccac gggccgctcg tcgagtgtgg cgagaaacgc ctccgcgtgc
      661 gctgcggcag agcgaaggcc tgcggtgcgg tcagccatga gccgacccta cgtcaggaca
      721 gggtggcggc gatctcgtcg aatgccgcga tcgtggggaa cagcttggtc gcgtcctgcg
      781 gcagtggcca gctcgacagc ttggccgcta cgagattcgc cgcccggttg acgtagatca
      841 tctggccgtg aatccccaag cacagcaaca cattgttgcc ggggtagggg aaccagaatt
      901 ggttgcggta catgccgccg ggcatccggt tgtcgtcggg gctggccgcg aacgccgccc
      961 gcgagtcgac gccgccggag aatgtgtcgg cgatccacga cagcgacacc acgcgctcgc
     1021 cagtcagcga cacgccaccg ttgaggaaca gcgacccgaa ccgggccaga tcgcgcaggc
     1081 aggcgttgat gccgccgtcg aacatgccgg tgccgaccga gtcgacaccg atggtggcgt
     1141 cgttgtccgc accgagtttg ctccacagca gctgcgacat cagctcgggc atccgaatcc
     1201 cgctgacggc ttcgcagatc cagcccagca cgtccgtctc gcacgatcgg tactggaacg
     1261 ggccgccgtg gggcgccttc tgtttcaacg tgagcaggaa gtcgtacatg gtggtcggca
     1321 gatccggcac cgttcgcggt gcccagccga tggcctgctc gagcagccgc acgtccgcca
     1381 gcggattcag ataatcctca gaaaacgcta tgcccgaacg catgtcgagc agatggcgca
     1441 ccgtcgagcc ggcatacccc gagttcgcca aaaccggaac ataggtcgtc agctccgcgt
     1501 cgacgttgag cgcgccgtcg ctgaccaatg ctcccgccac catcgcgatc aacgacttgc
     1561 tcaccgacat cagcaggtgt gaggtgtcgg cggtcatgcc gctgtagtaa tgctccgcga
     1621 ccaccttgcc gtgctgggtg acgatccagc cgtctgtcac ggtggccgcc atcacatcgc
     1681 cgacggtggt ccgcttgccg ttggtgcggt cgagcagcgg gatgtccgac agctcgctgg
     1741 gtgccgaggg caggttggcc accgggccgg tcccgcgcga gatcacggcc gtcggcagga
     1801 actcggcgat gtgctggaac gtccagtgca gctgggccgc cgactgccag ttctccaggg
     1861 ttacgtcgga cggcacgcgt ccaggatcgc cgccgagaac catggcgacg actgtagtgg
     1921 cccccgcgcg ccaccccgat gctcagccct acttcactcc cacatccgtg cgtgctgtcg
     1981 cgggctcggc gatcggttca cctgcgtatt cggcccggat cgagtcgatg aggtggcgcg
     2041 cccggatcgc cagcgacgat ttgccctggc cgctgtcgac ctgcagccgg cccttggtca
     2101 ccagcacgcc gaggtcggcg cccttccacc tgtagtcgcc gggtccgtag atgcgcagga
     2161 agaaggcctc gcgttcgttc tgcagctggt gccagtccag cgcggaccgt cggaacacca
     2221 ggccaccgct ggcgtcgagc gaatactgtc cggtgcgcgg tgtggcggtg agccgttgca
     2281 cgatgtcgtc ggtctccttc atcaccatct ggctccacac gtcggccgac gccagctcct
     2341 cccagcgttc gttgatctcg tcgacgtcga ggtcgaagtc gacgttcatg tattcgagga
     2401 tgtggaagag gaacagcggc acgtcggcat aggcgcccgc ggtgacgttg gtgatcgacg
     2461 aggccccgct tatccgcggg ttgagctcgc cgaggtacac gtcatttgtg tcggtgtcga
     2521 ccaggacgtc gacctcgaag aacccccggt agccctcctt ggccagccgg tctccgaggc
     2581 ggcgcaccaa ttgcgttgcc gtgcggcggc tttcaccggt gaggacttcg gggaacatct
     2641 cgttgccgca ccagccgccg cgggacgggg tcagccgttt gtagccgatc aactcggaca
     2701 tgaacgggcc cacgaccgtg ccgcatcgag tgagcaccgc ctcgaccgcg accgggcggt
     2761 tgttgatgcg gcgcatcacc ttgatctcct cgccgacgat atcggcgctg tgcttgcgcc
     2821 aatccgcctc cgaggagacg aagaacgtgg tcttaccgga gtcgccatac ggcgtctgga
     2881 cgacgagttc gtcaccgaga cgggcctttt gggcgacggc ctgcaggccg gcccagtcgt
     2941 cgacgcgggt cagcacattg ggcacgctgg gcgcgccggc ctcgttaccc agccgggtgg
     3001 tgacgatctt ggagtccagg tgctgacgca ggctggcggc gggcaggatc aggtcgtaac
     3061 ccagctcctt gcagatgtct tcggtctcgc tgttgaagaa caccatcgcg accttgggcc
     3121 gcacaccgcg cggtgtcgcg gcgtcgatgt gggcccgcac ctcggcgctg gtcagcagcc
     3181 agttgttgat ctcctcgccg ctctcgaact cgcggtacgg cttgtgggcc ggtgtgaaca
     3241 cgcgcggatg cgcgccgtcc cacgcgtcgt aataggtgat gtacgaaaag tttcggaccc
     3301 agcggtcgag gcccaaaagg ttgaacggcg tggcgccgaa gaacaggatc ggcacgtcgt
     3361 tggtgcggaa gaagtgccgc acttcggaga tgttcttcag ccgccgccgc ggcggggaac
     3421 cggatgcgaa ggccgcgtcg gtcccgatgc tgacctcgcg cggcttggcc gtggccctgg
     3481 cacggcgcgt ggttttcgcg gctgcggctt tggtggggcg tttcgctgga gttgatctgc
     3541 cggcggactt cttcgcggtg tcttttttcg tggctttctt ggtggctgtc gaaggccttc
     3601 tcgctcggac cgggcgatca ctcatggcgc gcacctcgct ctcgtcggat accccgacag
     3661 taggccgctg ggcagcggaa tggaggtggt tggcgcaggc tgcacgatcc ataactggac
     3721 gtatatagct atccatttcc tccagctatg acggcagcgc ttagcgcacg gtcgagatgc
     3781 tcgagtgcta gctgaactat gggaatagct gtgcagcaat cgctttcgca agattgcgtc
     3841 gctatcttcg tcggcgatgg gcattcgcag agctgacggc gcacccggtc gcactctgcg
     3901 cgcttggcgt actcgatgac tgtgccggag cggtgctccc ctgtgaaacg gctgtgaagt
     3961 ggcaaaacct ggtctcagat tgccgctata taggtcaggt gatatagata cggtgtggac
     4021 aaacagtcgg ctgatcacca cgccggcgac tgcgttggcg agctcctcga ccaggcgatg
     4081 gacctgaccg cgcggtttct gagcgatcgt gccgatttga gcgcatctgc cgcgtttgcg
     4141 atgaacaggg tgtgccgcga aggaccaatt cggcttacgg cactggccgc aaaggaaggc
     4201 gtcagccagc cgtccatgac tcagctgatc cagcggctgg agcggctgga cctggtggca
     4261 aggcttgcag atcccgacga tgggcgcgcc gccctcattg gcatcaccga acgtggtcag
     4321 gcgctgctca acgaccggaa gcggatgcgc cggcaacggc tgaaggcgtt gatggcgact
     4381 ctgacgccgg aagaggaatc cgccctgttg ctttcagcgc gggtcgcact cccggtactc
     4441 cgccggcttg tcgagaacgc ggactgtccg gccgacggtg aagcggcgca acaggccacg
     4501 accgagacgg cagggcggaa ggtgttgggg tgaaggctgt tccggtgggt cgcgcgctga
     4561 agcgcctgtg gatccccgtg cttctggtgg tcgtgttggc ggtctcaggc ctggtcgttt
     4621 cacgactgca caagatgttt gcgtcccagg acttgaatgc caacgccggc gcgggtattg
     4681 aaatcgtcca gttcaacccg aaggtcatgg tctacgacgt gtacggctca cctggaacca
     4741 cagcccagat cagttatttc gacacagacg cgaacgtgca ctcgatcacc gcgccgctgc
     4801 cctggtcggt caccctgtcg acgacgttgc cgacggtcag cgccagcctc atggcgcaga
     4861 ccgacggcga tcagattgga tgccgcgtca ccgtgaatgg aaccgtccgt gaggagcaat
     4921 ccgccaatgg ggtcaacgcc cagacgtact gcttggtgaa gtccgcatga caacaaccac
     4981 caccgctgcc gggcacgaga agcggcccgt catcgctcac agtcttcgga tccttgcggt
     5041 gccgatcatc ctgttctgga ttgccgtcgc cgtgctggtg aacgtgctcg caccgcaact
     5101 cgaggtcgtc ggtgagctgc actcggcccc aatggcgccc gaggacgcgc cgtcgatggt
     5161 cgcgatgaag ctgatgggcg ccaacttcaa ggaattcgat tccaacagca cgatcatgat
     5221 cgtggtggag ggccagaaac cactcggccc cgacgcgcac gcctactacg acgagatcat
     5281 ccgcaagctg cgccaggatc ccaagcacat ccagcacatt caggacttct ggggcgacac
     5341 cctgacggcg gccggggcgc agagcgccga cggcaaggcg tcctacgtga tgctgaacct
     5401 cgccggagaa cagggccaga cattggccaa cgaaggtgtg gagtccgttc gcaaggtcat
     5461 cgaggagacg aaagctccgc cgggagttca ggcctacgtc gcgggccccg ccgcgctcac
     5521 cgacgatctg cacgtcatcg gcaacgccag cctggctcag atcaccctgt tcaccctgat
     5581 cgcgatcgcg ggcatgttgc tggtcgtcta tcggtcgatc aggaccacgt tgatccagct
     5641 attcctgacg ttcatcggat tgctgacggc gcgtggagtg atatcagtcc ttgccagtca
     5701 cgacgcattc ggactgacga ccttcgccgg aaacatcctg acgatgttgg cgatcgcggc
     5761 ggccaccgac tacggcatct tcatcttcgg ccgctaccgc gaagcccgcg cgtccggtct
     5821 ggatcgagat gacgcctact acaccacttt caagtccgtc gctcccgtca tcgtggggtc
     5881 gggcctgacg atcgccgggg caacatactg cctgagtttc gcgcggttgc cctacttcag
     5941 cacgatgggc gcacccgtcg cgatcggaat ggtcgtggtg gtggcgtccg cggtcacact
     6001 ggggcctgcc gtccttttcc tgggcagccg tgtcgggttg ttcgaatgga agcgtccgcc
     6061 aaagggaagg ttctggcggc gggtcggcac catcgtggtc cgttggcccg caccggttct
     6121 ggtggccagc gcgttcgtgg tgctgatcgg cctggtcgcc gttccgggat acaagccggc
     6181 gtataacgac cgctactacc tgcctgccga cgccccgacc aacatcggat tcgcagccgc
     6241 cgaccggcat ttcacgcagg cgcggatgaa ccccgacatc ctgatggtcg aggccgatca
     6301 cgatatgcgg aaccccgccg acatgcttgt gctgaacaag atttcaagca atgtcatgca
     6361 caccgaaggc attgcgatgg tgcagaacat cacgcggcca ttgggaattc cgatccagca
     6421 cagctcgatt ccgttccaga cgagcgtcca gggccagacc aataacatga acctgccgtt
     6481 ccagcgggat caattggcca atcagctcaa aacgatcgat gagacgaacc gctcgatcga
     6541 catcttggag cagcagtacc agctctccct cgaacaaacc aagctcacac aggactcggc
     6601 ggccaagtcg caggccctcc tgcaaaccac cgaagaacta cgagacaaca tcgcgaactt
     6661 cgatgaccag ttccggccct tgcgcaatta cttctactgg gaaccgcatt gcttcgacat
     6721 ccccttgtgc aacgcaacac ggaacctgtt tgatgcgctc gatgggatag accgagtgac
     6781 ggaccagacg ggggcggtgc aggtcaacac cgaccagctg gcggacctcg ctccgaagct
     6841 caccgccctc cttccacaga cgatcgcgtc gatgaaggcc agcagggacc tgtcgctggc
     6901 gtcgtacaac tcgcagaagg ctttgctcga ccagctggag gcctccaaca acactgccct
     6961 tgcgatgggc gcggccttcg acggggcgaa gaacgacgac ctgttctttc tgcccccaga
     7021 ggcattcgac aaccccgatt tcaagcgcgg gctggtgatg ttcctgtcgc cggacggcaa
     7081 gtcggctcgc atgttcatca cgcacgagag cgatccggcg acggtcgatg gcatcaagcg
     7141 ggtggagggg gagaggaagg cagctcagga agccatgaag atgtcctcct tgtcgaacgc
     7201 caagatctac ctcggtgggg tcgcggcgac ttataaggac atggctgacg gcgccaggta
     7261 cgacttgctg atcgccgtgg tgtcgtcgtt gacgctgatc ttcatgatca tgctcatcct
     7321 cacccgcagc gcggtggccg ccttcgtcat cgtgggcacg gcggcaagtt cgatcgccgc
     7381 gtccttcggc atctcagtgc tgttgtggca ggacttgttc ggcatccagg tgcagtggct
     7441 cgtcatgctg atgtcggtca tcatcctgtt ggctgtcggg tcggactaca acctgctgct
     7501 ggtgtcccga ttcaaggacg agatccacgc ggggctgaag acaggcatca ttcgctcgat
     7561 ggccggtacg ggcggcgtgg tgacgtccgc gggtctggtc ttcgcggcca cgatggcggc
     7621 gatgatgggc agcaagctgg tcgtgctggc gcagatgggg tccacgatcg cgatcggcct
     7681 actggtcgac acgttcgtcg tccgctcctt cctgatgcca tcgattgcaa cgcttttggg
     7741 ccggtggttc tggtggccgc aggtcgtcta tccccgcggc gactatcact tcctgccacc
     7801 gcaaccccgg cgccgggcca cggacgaccc cgacacggtg gcgctgccgg cgcaggcgta
     7861 aaggaaaccg cctatgcggc agactgcttg atcgccacga ggaagtcgga gacgagccgg
     7921 ttggtggccc gccgccagac ggcagcaagg tcgatcgtcg tcgaccccga cacccggatg
     7981 actttcaccg aacttctgag ctggtgggcg c
//
LOCUS       DASRJA010000666        17676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1781394,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000666 DASRJA010000000
VERSION     DASRJA010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17676
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781394"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3888
                     /locus_tag="VFC01_29840"
     CDS             <1..3888
                     /locus_tag="VFC01_29840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN83851.1"
                     /translation="VRLADVAHTLNHHRAQHSKFATVTASDSAQAVAGLRALAGQYPA
                     PGVVGPHDGTCRSGTVFLYSGQGSQWAGMGRRLLAEEPAFGAAVAELDPVFTEQVGFS
                     LREVLEAGQTVVGIDRIQPVLVGVQLALTALWRSYGVNPDAVIGHSMGEVTAAVVAGA
                     LSPADGLKVIATRSRLMSRLSGQGAMALLELDSDAAEKLIADYPGITVAVYASPHQTV
                     IAGPPEQVDAVIAVVDARDRLARRVDVDVASHHPIIDPVLPDLRAELADLKPHSPRIP
                     VIVTTGHRAADGTNVFDAEHWAANLRNPVRFAQAVATAGANHAIFVEVSPHPLLTYAI
                     SDTLEETHHHAIGTLQRDTNDTMTFHTNLNATHTIRPRELAHPAEPHLLIPTTPWHHT
                     RHWITTPEAPETLHHNGVAVPSAAGSANTGGAYHEWLYQMAWPIRGLAATEKSSAGSW
                     LVLAEPDAASELGQLLGPDSRALAQSALAKDGDIAALRDALSGSDNVVFAPSVLSARL
                     DITEGYRLFNEVRRLVATLVGMTSPPKLHIVTRNAQPIGEGDRANPAHAVLWGLGRTL
                     ALEHPEIWGRIIDVDDSAPPELIAQHLLSEVRTSDGDDQVVYQGGVRHVPRLERQPAP
                     AVSLTRLEGDTSHLVIGATGNVGPYLIRQLADMGAATIVAVSRRGGTQLAALASELAS
                     GGTELIEVAADATDEAAMSALFTRFGTDLPPLDGIYLAALAGGEALISEMTDEDVNTM
                     FRSKLDVAAVLHKLSLKRPVRRFVLFSSITGILGSRLLGHYTAANTFLDTFAYARRAL
                     GLPATVVDWGLWKSWADAQPATTAAGLQPMPNDVAIRMLAMVLSPDARVQSAVVGADW
                     DRLAEAYRMRGSLRVVDHLLADAAPGQPNIDDVPAPLPGTLLGEQVATTAHEQLWRAR
                     LVPEAKPYPGGHRVRGVEVVPVSVLLQTLSAAAAQCGASTVCDIRFEYPIVADGQRVI
                     HVVADGESLTVSSSPGADTPANRWTRHVSAHIAERLSDDAPGGADIGEVEDVPGFDGL
                     SIAELQRAWGIEGQPFGWSVGTCGSTPGGVRAEVSLPEASTVALLDAAVHLARLADST
                     NPQLMLPAAVESIRSAAEFADQGGLVQVSRRGDSGDELIVDVVAKALDGTTTLEIRGL
                     RYAAMESAPAHAAPETSSAPVEMPAWSEMSPEKMLGELQIRLCRILARELGMPEAAVN
                     TDTPFPELGLDSMMAMNLLRDAKQLVQIDLSATMLWNHPTISSFAAYVTELLAPEEQP
                     EEEHIDVTSESISVLDALFESVESAPAGSESGI"
     gene            3888..9296
                     /locus_tag="VFC01_29845"
     CDS             3888..9296
                     /locus_tag="VFC01_29845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HZN83852.1"
                     /translation="MKTTFDRISAMTADQRGALADQFDKASRIAAAEPVAVVGIGCRF
                     PGGVAGPEAYWEFLANGTDAVTEVPSDRWDGDAFYDPDPMAPGRMPTKWGAYLSDVSG
                     FDADFFGISPREAVAMDPQQRVLLEVAWEGLEHAGIAPDNLGETRTAVMMGVYYTEYQ
                     NASAGNPDTIDPYSATGNAHSVTVGRISYLLGLRGPAVAVDTACSSSLVSVHLACQSL
                     RMRESDLALAGGVSLNLRPETQLALAKWGMLSPHGRCHAFDSRADGFVRGEGAGVVVL
                     KRLTDAVRDGDRVLGVVRGSAVNQDGRSNGLTAPNAPSQRDVITRALRSADVAAGSVN
                     FIETHGTGTALGDPIEFDALAAVYGRAGVPCALGAVKTNFGHLEAAAGIAGFIKAVLA
                     LQRAHIPPNLNFKQWNPAIDHASTRLFVPTESAPWPEHDGPRRAAVSSFGMGGTNAHV
                     VLEQGPDPVPVSAEDSGPAVTTLVVSGKTAQRVASAAGVLAEWMEGAGSGVPLTDVAY
                     TINHHRSRYSTLATISARDRRQAVAGLRALAAGQPAPGVVPSHDGSSGPGTVFLYSGQ
                     GAQWPGMGRQLLADEPAFAVAVADLEPVFVEKVGFSLRQVLDAGEPLVGIARIQPVLV
                     GMQLALTELWRSYGVEPDAVIGHSMGEVTAAVVAGALSPAEGLDVIATRSRLMSRLSG
                     QGAMALVELDAAAAERLIADHPDVTVAVYASPRQSVIAGPPDQVDALVASVDAQGLLA
                     RRIEVDVASHHPTIDPILPELRTALSYLAPNTPKIPLISTTGYIGATPLFGADYWLAN
                     LRNPVRFSQAVATAGAEHSTFIEISPHPLLSHAISETLAAATPNKSVHVGATVNRDNP
                     ETVSFHTQLAAVRPPVDGIAQSNGSAGRLADIPPTPWLHSKYWVTDRSVGREYTGAHP
                     LLGMHVELPSGGAHVWQSDVGTELNPWLDDHKVHGQPVMPGAGFAEICLAAGCEALGL
                     PVGSVEVKRLEVEQMLPLDGRTRLTTQFIRDGDGSDRVEIHSRSIGGNWTRHAVARVD
                     IAQHDVRPGALGSPPEGGTTASPADFYTAMRRTGAHHGQAFAALTRIVRAPGGWADTE
                     IVLPDEATPYRGSLLHPVMLDAALQSLAAAMPTESLSDSTEVTYLPVAMESIRVFGEV
                     GRRARCRAELVSIVEDSGDALGRVTLVDDTGTPTAEVTGVYVRRVQRRTVPLPLSQKV
                     FETSWVQTPPAPLASAPTGSWLVLTDNAESAALAKEFTASFASVTRRINTADLADESA
                     VLEAFAETAADPELPPAGVVVLIGSRSFDETDVDGALGHARDLVWSMSTAVRAIINGW
                     HGKAPRVWMVSRNGLAVDGEYGNPGIGTLRGLVRVLAYEHPDLRTTLLDLDATEDAVA
                     TLVTELEASDNDDVIAWRGEHRYVERLSRAALPGPWGDPVVRRDGSYIVTGGLGGIGM
                     TVARWLVDSGAGRLVLNGRSAPSDEMRNHLAELGTRAEVVTVLGDVAEPGTADRLVAA
                     AEQTGRPLRGVIHSAAVLDDELVVGLTKDSLDRIWTPKAAGAVRLHGATASSQLDWWV
                     GFSSVVSLLGSPGQASYACANAWLDALVEWRKAAGLPATAINWGQWADVGLSRAMQFS
                     ALDPMSPAEGVDALESVVASGITRVGVARLRLDRAAAAFPEIHQIGFFTNLVAELGDL
                     DADDDWGGTDALREMDPADVNRVVIARLRKRISAIMGFSGDGAVEIGQPLTELGMDSL
                     MAVRMRNTIRGDFGVEPPVALLLQGATLADLALDLIRQLGLAEQENTERHNAVRDRAQ
                     QRAAARQRAASRRKVGQR"
     gene            9293..13720
                     /locus_tag="VFC01_29850"
     CDS             9293..13720
                     /locus_tag="VFC01_29850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZN83853.1"
                     /translation="MTSGIDFTSAANSLPPNAIAVVGMAGKFPGADSVSAFWRNLRAG
                     VESIVDLSEDELITAGVGEKALANRSYVRRAALLPGIEEFDAEFFGFTPQAARMMDPQ
                     HRLFLQTVFHALEDAGYDPLQIDNTVGVFGTSSASGYLLHNLMSHYDPNLVIGQGASF
                     DMVNLSLQNDKDHLATRVAHQFNLRGPALAVQTACSSSLVAVHLACQSILNGECDIAL
                     AGGSSLRVPHRVGYWYEQGSMVSPTGHCRPFDVRSDGTIFGSGVGVVVLKPLQDAVDD
                     GDRIHAVIRGSALNNDGSTKMTYAAPNAVGQAEVIAEAHAVADIDASTVSYVETHGTG
                     TPLGDPIEIEGLRQAFEVSTETRPGPCYVGSVKSNIGHLETAAGIAGLIKAILCLEHR
                     AIPATLHYTSPNPELHLDRGPFVVRSEDGAWEWDGVLRAGVSSFGVGGTNAHVVLEEP
                     PSVPAAQTRPGPQVLLLSARTAESLQQSRTALATELSGVDEVSLPDVAYTLTRRRKEP
                     VRLAAVVNDQEHAATVLGATEHDNVFVGAAIPQAESHSDRVAFVFPGQGAQHIGMARG
                     LHECEPIFAEQFDACAAAFGDEMGFDLRAEVFDGAGRNLERTDRAQPALFAVEYALAK
                     LVESYGVRASVLAGHSIGEYVAATVTGVFDLPTAVKAVSMRARLMHASARGVMVAVAL
                     SPDAIAEYLGSDVDLAAVNDPGSCVVAGSEERIRDFQARLAENGIVARRVRTAHAFHS
                     RLMDPVIPEFNAFMSRLTLRDPQIPLLSNITGTLMSGGEATNPATWARQIRATVRFSD
                     ELDELLADPTRVLVEVGPGGALTGSAVRHPKWSDGHRAVRLMRHQAQNRSDHDTFLLA
                     LGQLWSAGVEVDWSPRLTRRSRLVSLPGYPFERQRHWVQHNAGAAWGAGATPTNGTAA
                     APSAAGNTSAPTSSTSQMESTLQRIWSQCLGIGSIDRTANFFELGGDSLIAISVAMTA
                     SKEGLDLTPQDLYENQTVAALARVLVARYAEGGLGRQSLSDAVNPPLPPNVAHFLEHG
                     LRDVGRWRIPVILHLRPDVDVEDVRSALTAVTNHHDALRLRVVERAGTRDQGVAEPDA
                     FTELVTRALPEGIAAGSPQERDAVLAILDEQIREHQLMSAPLTATYIRGLPGGHSYLT
                     ISLHGIAGDETSRDILLTDIFTALNQRVAGQDIVLQPATTSWREWSQRCAGLATHPAV
                     METRDYWLATATKATLRVAGPEASEPPGTDDLTRLSAALTLSETGEIDDARRRLRLPV
                     EEILLAALGRTVAGTVGEGAVAVDLGGRGRSVLKPDVDLQRTVGWFTTIYPIVLRCAT
                     TQEASARQLLDDVRGTLNAVPHYGIGYGLLRYMYAPTARLLGATRGPDILFTHVGTIP
                     DLPSEQSDDAPVQFDADTALPVRDSVPGLGHAIELRVYRSSGVLHLDWWYDTRRLGPT
                     DMESLARQFSSALMELTREALAENEIDSGGDELALVDLS"
     gene            13744..14739
                     /locus_tag="VFC01_29855"
     CDS             13744..14739
                     /locus_tag="VFC01_29855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN83854.1"
                     /translation="MSSTEKAVIVSGISKTFGSVVAVRDISFDIGVGEVLGLLGPNGA
                     GKTTTVDVLSTLTRPDHGVAVIAGHDVVSDPAGVRRSIMLTGQHVSLDDMLTGRENLV
                     MFGRLQGLKKGEARSRAKELLEQFDLEDAGDRNVGTYSGGMKRRIDIACGLVVRPEVV
                     FLDEPTTGLDPRSRQAIWELVGSFKKQGIATLLTTQYLEEADLLSDRIVVIDHGTVIA
                     EGTADQLKERTGGTYCEIVPCHMDDIPAMAEALGSLLPESNRAALTDASDRIAMPAPD
                     GPTTLMQALNLVKEANIELVDIALRRPSLDEVFLALTAHDTRNTSEPRVEAEAVS"
     gene            14736..15554
                     /locus_tag="VFC01_29860"
     CDS             14736..15554
                     /locus_tag="VFC01_29860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN83855.1"
                     /translation="MTDTTTAELTVRPTASTIQQWWVLTVRMLTPTLRNGELATQIVG
                     SIVFTIGYYIPLKQIMGAAQGMSSYAQYLTPLIVLQAIWFAAVSAAFRSATDSVQGIN
                     RRFKAMPIASLTPLASRMSASMYRCCVALTVSVACGHVIGFRFHDGVVHAVGFCVLVL
                     LIGAALAFIGDLIGVATQNPEATAPLMLVPQVTLGLASTGLQPVERFPEWIQPFVRNQ
                     PLSQFVYALQALAGDSTSAAPEVRWSVIGPALAWVAGMILISVGLHMRVSKRRP"
     gene            15554..16444
                     /locus_tag="VFC01_29865"
     CDS             15554..16444
                     /locus_tag="VFC01_29865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN83856.1"
                     /translation="MSVASQDKKTIAVGRAAGTQVETRTHPAPGRHRLPRPRENSPRR
                     LIPHTWALTARILRRWSRDPATVVQSLVFPAGFLVALDIVLGDGVEQVTGHSALYGSV
                     PLVATVGATSGAIIGAVGLMRERSDGLLSRLWVLPIHRASGVMARLTADAIRIVVITT
                     VVLGVGLMLGFRFKQGIGEAALWLFIPALFGVAFSSAVITLALYSSNTIVPQATEIIC
                     AMLFFFSTGFVPLDQFPSWIQPIVEHQPISYVIETMKGLSLGGPVLAPLVATLLWSAG
                     IAALCAVPMAIGYRRASKRG"
     gene            16484..>17676
                     /locus_tag="VFC01_29870"
     CDS             16484..>17676
                     /locus_tag="VFC01_29870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZN83857.1"
                     /translation="MFPSSVLRKLARSEEMFAETHNFVGLGAHVEGPVDVDALSDAFD
                     ALLQAHPALAGHLEQDPDGRYQIVVDDLLHPGIEVIELDDPAAKTPPLYFDQTASLAH
                     FRLTIRDGQAQPTLYIHHSLADGHHQFSLVEELFSYYTDLVTTGTTRPLDVHPVPDPL
                     EVVLADRGVQKVNRSGLERLLTAMFVYDLPPSRRTASEVKPAWPQLVPMAFCRLSERE
                     TDDLVAFCRVHKVGLNGLLSAVVLMAEWRLRATPKIPVPYVYPVDLRYVLSPPVSATA
                     CTNPVGIATYLAEIDHNTDVVDLARDIADTFRKDLSEGVIQQSFLHFSPQYVGNPPGL
                     PDVVMFTDNGVVPPIRTPPEMKITASHGEFYFGVSAGIEIYTSKIFAGQLMIEYHSHG
                     PEPEKS"
BASE COUNT         3030 a         5557 c         5839 g         3250 t
ORIGIN      
        1 gtgaggctgg ccgatgtggc acataccctc aaccaccacc gcgcccagca cagcaagttc
       61 gccaccgtga ccgcatctga cagcgcgcag gcggtcgccg gtttgcgtgc gctcgccgga
      121 cagtacccgg cgccgggcgt agttggtccc cacgacggga cgtgccggtc gggcacggtg
      181 ttcctctatt cgggtcaggg ttcgcagtgg gccggaatgg gccggcggtt gctggccgag
      241 gaaccggctt ttggggccgc agtcgccgaa ttggatccgg ttttcaccga gcaggtcgga
      301 ttttcgctga gggaggttct ggaagcgggt cagacggtgg tgggtatcga ccgaatccag
      361 ccggttctgg tcggcgtcca gttggcgctt actgctctgt ggcgttccta cggggtgaat
      421 cccgatgcgg tgatcgggca ctccatgggg gaggtgaccg cggccgttgt ggccggtgcg
      481 ctcagccctg cggacgggct caaggtgatc gcaaccaggt cgcggttgat gtcgcggttg
      541 tccggccagg gcgcgatggc actgttggaa ctggactcgg atgccgccga gaagctgatc
      601 gccgactacc ccggcatcac cgttgcggtg tatgcctcac cgcatcagac cgtgatcgcc
      661 gggccacccg aacaggtcga tgccgtgatc gcggtcgtcg acgcccggga ccggctggcc
      721 cgtcgggtcg acgtcgatgt ggcgtcacac caccccatca tcgatccggt gctgccggac
      781 ctgcgcgccg aacttgctga cttgaagccg cattcgccgc gcatcccggt cattgtcacc
      841 accggtcacc gagcagccga tggcacgaat gtgttcgacg ccgagcactg ggccgccaac
      901 ctgcgcaacc cggtgcggtt cgctcaggcg gtggccacgg ccggtgcgaa ccacgcgatc
      961 ttcgtggagg tgagcccgca cccgctactc acctatgcga tctccgacac gctggaggaa
     1021 acgcatcacc acgccatcgg aacactgcag cgtgacacca acgacacaat gacgttccat
     1081 acgaacctca acgccaccca cacgatccgc ccccgcgagc tggcgcatcc ggccgaacct
     1141 catctgctca ttcccacgac gccatggcac cacactcggc actggatcac cacgcccgaa
     1201 gcgccagaga cgctgcatca caacggagtt gccgtgccat ccgctgcggg atcggccaat
     1261 acgggcggtg cgtatcatga atggctctat cagatggcgt ggccgattcg aggactggcc
     1321 gcgaccgaga agtcaagcgc aggttcatgg ctcgtgcttg ccgagccgga tgccgcgagt
     1381 gagctaggcc agctgctcgg accggattcc cgcgcgctcg cacagtccgc gctggcgaaa
     1441 gacggtgaca tcgccgcact tcgtgatgcg ctgtccggct ccgataacgt ggtctttgcg
     1501 ccgtcggtgc tgtccgcacg ccttgacatc acagagggtt accggctgtt caacgaggtg
     1561 cggcggcttg tcgcaacgct tgtcgggatg acgtcaccgc cgaaattgca catcgtcact
     1621 cgcaatgcgc aaccgatcgg cgaaggagat cgggctaatc ccgcgcacgc ggtgctctgg
     1681 ggtctgggtc gcacgctggc gcttgagcac ccggagatct ggggacgcat catcgacgtc
     1741 gacgactcgg ccccgccgga gttgatcgcg caacacctac tttccgaggt gcgaacctcc
     1801 gatggcgacg accaagtcgt ttatcagggc ggtgtccgcc atgtgccgcg cctcgagcgg
     1861 caacccgcgc ctgcggtgtc gctgacgagg ctggaggggg acaccagcca cttggtgatc
     1921 ggtgccaccg gcaacgttgg gccgtatctg atccgacagc tcgccgacat gggcgccgcg
     1981 acgatagtgg cggtatcccg ccgtggtgga acgcaattgg cggccttggc ttcagaactc
     2041 gcatcgggcg ggaccgagct catcgaagtt gccgcggacg ctacggatga agccgcgatg
     2101 agtgcactct tcacgcggtt cgggacggac cttccgccgc tggacggaat ctacctcgcc
     2161 gcgttggccg gcggggaggc gctgatcagc gaaatgaccg acgaggacgt caacaccatg
     2221 ttccggtcca agctcgacgt cgcggcggtg ctgcacaagc tgtccctgaa gcgtccggtg
     2281 cgccgattcg tgttgttctc ctcgatcacg ggcattctcg gctcacgatt gctcggccat
     2341 tacaccgcgg cgaatacgtt tctcgacacg ttcgcgtacg cgcgccgagc attggggctc
     2401 ccggcaacgg tcgtcgactg gggcctgtgg aaatcgtggg cagacgcaca acccgcaacg
     2461 acggccgccg ggcttcaacc gatgccgaac gacgtcgcca tccggatgct ggctatggtg
     2521 ctcagcccgg atgcccgtgt gcagtccgcg gtggtgggcg ccgactggga tcggctcgcc
     2581 gaggcttacc gcatgcgcgg ctcgctgcgc gtcgtcgacc atctgctggc cgacgccgca
     2641 cccggccaac cgaatatcga cgatgtgccc gcgcctttgc ccgggacact actgggcgag
     2701 caggttgcca ccaccgcgca cgaacaactg tggcgggcgc gactggtgcc cgaagccaaa
     2761 ccctatcctg gcggccatcg ggtccgcggt gtcgaggtcg ttccggtatc ggttctgctg
     2821 caaacacttt cggccgccgc ggcacaatgc ggcgcatcga cggtctgcga cattcgattc
     2881 gagtatccga tcgtcgccga cgggcaacgg gtgattcacg tggtcgccga cggcgaatcg
     2941 ctcacggtgt cctccagtcc gggtgcggac accccggcaa accgctggac cagacatgtc
     3001 agcgcgcata tcgccgagcg gctctccgac gacgccccgg gtggtgctga catcggcgag
     3061 gttgaagacg ttccaggctt cgacggcttg tcgatcgccg agcttcagcg ggcgtggggc
     3121 attgaagggc agccgttcgg ttggtcggtc ggtacatgcg ggtcgacgcc cggcggggtg
     3181 cgcgccgagg tctccctgcc agaggcatcg acggtcgcgc tcctcgacgc cgccgttcac
     3241 ctcgcccgcc tggcggacag caccaaccca cagctgatgc tccctgccgc cgtcgaaagc
     3301 atccgttccg cagccgaatt cgccgatcag ggagggctcg tacaggtgag ccgccgcggc
     3361 gactccggtg acgagctcat cgtcgatgtc gtcgcgaagg cactcgacgg cacgacgacc
     3421 ttggagatcc gcgggctccg gtacgcggca atggagtcgg ctcccgccca tgccgcaccc
     3481 gaaacatcgt ctgccccagt cgaaatgccc gcatggtcgg agatgtcacc ggagaagatg
     3541 cttggcgaac tgcagatcag gctgtgcagg atcctggccc gcgagctcgg gatgcccgaa
     3601 gcggcggtga atacggatac cccgttcccc gaattgggcc tggattcgat gatggcgatg
     3661 aatctcctca gagatgccaa gcagttagtg cagatcgact tgtcggccac gatgctgtgg
     3721 aaccatccga cgatctcgtc gttcgcggca tacgtgacgg aactacttgc ccccgaggaa
     3781 caacctgagg aagaacacat cgatgtgaca tcagagtcga tcagtgtgct cgatgcgttg
     3841 ttcgaaagtg tggaatcggc tcctgccggt agtgagagcg gtatttgatg aagacgacct
     3901 tcgacaggat ttcggccatg accgccgacc agcgcggcgc gctggcggac cagttcgata
     3961 aggcgtcccg catcgcggcg gcggaaccgg tggcggtggt cggaatcggg tgccgtttcc
     4021 ccggtggtgt ggccggtccc gaagcgtatt gggagttcct ggccaacggc acggatgcgg
     4081 tcacggaggt gccgtcggat cggtgggacg gcgatgcgtt ctatgacccg gatccgatgg
     4141 cgcctgggcg gatgccaacg aagtggggcg catacctgtc cgacgtgtcg ggtttcgacg
     4201 ccgacttctt cggcatctcc ccgcgcgagg cggtggcgat ggatccgcag cagcgagtgc
     4261 tgttggaagt ggcctgggaa ggcctcgagc atgccggtat cgcgcccgac aacctcggcg
     4321 aaacccgtac cgcggtgatg atgggcgtgt actacacgga gtatcagaat gcttcggcgg
     4381 gaaatccgga caccatcgat ccgtattccg caacaggcaa cgcgcacagc gtcactgtcg
     4441 ggcggatctc ctacttgctt ggcctgcgtg gtccggcggt ggcggtcgat accgcctgct
     4501 cgtcgtcgtt ggtgtcggtg catctggcct gccagagtct gcggatgcgg gagagtgacc
     4561 tggcccttgc cggcggggtc agcctcaact tgcgaccgga aacccaactt gcacttgcca
     4621 agtggggaat gctttctcct cacggccggt gtcatgcctt cgattcgcgg gcggacggtt
     4681 tcgtgcgtgg tgagggtgcc ggggtcgtgg tgctcaagcg gctcaccgac gcggtgcgtg
     4741 atggtgaccg cgtgcttggg gtggtgcgcg gctcggcggt caaccaagac ggtcgctcca
     4801 acggcctgac cgcacccaac gcgccctcac agcgcgatgt catcacccgc gctttacgct
     4861 cagccgatgt cgctgcgggt tctgtgaatt tcattgaaac ccacggcacc ggaacggctt
     4921 tgggcgatcc gattgagttc gacgcgttgg cggctgtcta cgggcgtgca ggtgtgccgt
     4981 gcgcgctggg tgcggtgaaa acgaacttcg gtcatctgga ggcggcagcc gggatcgccg
     5041 gcttcatcaa ggccgtgctg gcgctgcagc gcgcgcacat accaccgaat ctcaacttca
     5101 agcaatggaa tccggcgatc gaccatgctt cgacgcggct attcgtgccg acggaatccg
     5161 caccttggcc cgagcacgat ggccctcgcc gggcggcggt gtcgtccttc ggcatgggtg
     5221 gcacgaacgc acatgtggtt ctcgaacagg ggccggatcc cgtgccggtg tcggccgagg
     5281 actcgggtcc tgccgtgacg actctcgtgg tctcgggcaa gaccgctcag cgcgtcgcgt
     5341 cggcggccgg cgtgttggcc gagtggatgg agggcgcagg ctccggtgtg ccgctcaccg
     5401 atgtggcgta caccatcaat catcaccgca gccgttacag cactctcgcc acgataagtg
     5461 cgcgcgatcg ccgtcaggcc gtcgcgggtc tgcgcgcgct ggccgccggg cagcccgcgc
     5521 caggagtcgt cccttctcat gacggctcaa gcggaccagg cacggtgttc ctgtattccg
     5581 gtcagggagc gcaatggccc ggcatgggtc ggcagctgct cgccgacgag ccggcattcg
     5641 cggtggccgt cgccgatctt gagccggtct tcgttgagaa ggtcgggttt tcgctacggc
     5701 aggtgctcga tgcgggcgag ccgctggtcg gcatcgcgcg gattcagccg gtgctggtcg
     5761 gcatgcagct ggcgctgacg gagttgtggc gctcgtacgg ggtggaaccc gatgcggtga
     5821 tcggacattc gatgggggag gtgaccgcag ctgtggtggc cggggcactg agtcccgctg
     5881 aaggtttgga tgtcattgcc acccggtcgc ggttgatgtc gcggttgtcc ggccaaggcg
     5941 cgatggccct ggtggaattg gatgctgcgg cggcggagag attgatcgcc gaccatcccg
     6001 acgtcaccgt ggcggtgtat gcgtccccgc gtcagtccgt gatcgctgga ccacctgacc
     6061 aggtcgatgc gctggtcgcc tcggtggatg ctcaaggcct gctagcgcgc cgaatcgagg
     6121 tggacgtcgc atcccaccat ccgacgattg acccgatcct gcccgaattg cggaccgcgc
     6181 tttcatacct ggcgcccaac acgccgaaga tcccattgat cagcacgacc gggtacatcg
     6241 gcgcgacacc gctattcggc gccgactact ggctggccaa cttgcgcaat ccggtgcggt
     6301 tcagtcaggc ggtcgcgacc gcaggcgccg aacacagcac gttcatcgag attagcccgc
     6361 atccgctgct gtcccatgcc atctcagaga ccctggcggc tgcgacgccg aacaagagtg
     6421 tccacgtggg ggcgacggtc aaccgggaca accccgaaac cgtgtctttc catacacagc
     6481 tcgccgcggt tcggccgccg gtcgacggga tcgcgcaatc gaacggcagc gcgggccgac
     6541 tcgcggacat tccgcccacg ccgtggcttc attcgaagta ctgggtcacc gaccgctcgg
     6601 tgggacggga gtacacgggt gcccatccgc tgcttggcat gcatgtcgag ttgccttcgg
     6661 gcggtgcgca cgtgtggcag tccgacgtcg ggaccgagct gaacccgtgg ctcgacgatc
     6721 acaaggtgca cggccagccg gtaatgccag gagcgggatt cgccgaaatc tgtttggcgg
     6781 ccggctgcga agccctgggc ctaccggtcg gcagcgtgga ggtgaagcgg ctcgaggtcg
     6841 agcagatgct gcccctggac ggccggactc ggttgacaac acagttcatt cgagacggag
     6901 acggcagcga ccgcgtcgaa atccactcgc gttcgatcgg cggcaactgg actcggcacg
     6961 cggtcgcacg ggttgacatc gcgcaacatg acgtccggcc gggtgccctc ggttcaccgc
     7021 ccgagggcgg gacaacggcg tcgcctgctg atttctacac cgcgatgcgg cgcaccggtg
     7081 cccatcacgg tcaggcgttc gccgcgctga cgcggatcgt tcgcgctccc ggcgggtggg
     7141 ctgataccga gattgtgctg ccggacgagg ccactcccta ccggggaagc ctcctgcatc
     7201 cggtgatgct cgatgcggcg ctgcaaagcc tggctgccgc gatgccaacc gaatcccttt
     7261 cggattcgac cgaagtcacg tatctgcctg tcgcgatgga gtccatccgg gtgtttgggg
     7321 aggttggcag gcgtgcacgg tgccgtgccg agctcgtgag catcgtggaa gacagcgggg
     7381 atgcgctggg ccgggtcacc ttggtggacg acacggggac acccactgca gaagtcaccg
     7441 gtgtctacgt acggcgcgtg caacgtcgga cggtgccatt accgctgtcc cagaaggttt
     7501 ttgaaacttc ctgggtccag acgccgccgg ctccgttggc tagtgcaccg acgggtagtt
     7561 ggctggtgct tactgacaac gccgaatcag cagccttggc aaaggagttc acggcgagtt
     7621 tcgcctccgt gacccgacgg atcaacacag cggatctcgc cgacgaatcc gcggtgctgg
     7681 aggcctttgc tgagacggcg gcggatcctg agctgccacc ggcaggagtg gtggtgctca
     7741 ttggaagccg ttcgttcgat gagactgacg tcgacggagc gcttgggcac gcgcgggatc
     7801 tggtgtggtc gatgtcgaca gccgtgcgcg cgatcatcaa cggctggcac ggcaaggccc
     7861 cgcgggtgtg gatggtgagc cgcaacggtc tggccgtgga cggcgagtac ggcaacccgg
     7921 gcatcggcac cctgcgcggt ctcgttcgcg tcctcgccta cgaacacccg gatttgcgta
     7981 ccaccctgct cgatctggac gcgacggagg atgcggtcgc aacgctggta accgagctgg
     8041 aggcgtcgga caacgatgac gtgatcgcct ggcggggcga gcatcgctac gtcgaacggc
     8101 tctcgcgcgc cgcgcttccc gggccctggg gcgacccggt ggtgcgccga gatggctcat
     8161 acatcgtcac cggcgggctc ggcggtattg gtatgacggt ggcgcgctgg ctcgtcgata
     8221 gcggagcagg ccgtctcgtc ctcaacggtc gttcggctcc atcggacgag atgagaaacc
     8281 acctggccga gctgggaacc cgcgctgaag tcgtcacggt gctcggcgat gtggccgagc
     8341 cgggaaccgc tgaccgactt gtcgccgctg ccgagcagac cggccggccg ttacggggag
     8401 taatccactc ggccgcagtc ctcgacgacg aactcgtggt gggcctgacc aaggacagtc
     8461 tggaccgcat ctggacgccc aaggcggccg gcgccgtgcg actgcacgga gcgaccgcga
     8521 gcagccagct cgactggtgg gtcggcttct cttcggtggt gtcgctgctg ggctcgccag
     8581 gccaggcgtc ctatgcgtgc gccaacgcgt ggctcgatgc tctggtcgag tggcgcaagg
     8641 ccgccggact gccggccacg gcgatcaact gggggcaatg ggccgacgtg ggtctctccc
     8701 gggcgatgca attcagcgct ctggacccga tgtcgcctgc cgagggcgtc gatgcgctgg
     8761 agtcggtggt cgccagcggg atcacaagag tgggcgtggc acggctgcgc ctcgatcgtg
     8821 ccgccgcggc atttcccgag atccaccaga tcggattctt caccaacctc gtcgctgaat
     8881 tgggcgacct tgacgcagat gacgactggg gcggcaccga cgcgctacgg gaaatggatc
     8941 ccgcggacgt caaccgtgtc gtcatcgcgc gcctgcgcaa acggatctcg gcgatcatgg
     9001 gcttctccgg tgacggtgcc gtcgagatcg gccagccgtt gactgagctc ggcatggatt
     9061 ccctgatggc ggtgcgaatg cggaacacga ttcgcgggga cttcggcgtg gagccgccgg
     9121 tcgcccttct cctgcagggt gccacacttg ccgacctcgc actcgacctg atccgtcaac
     9181 tcggcctggc cgagcaggaa aacaccgaac gccacaacgc agttcgcgat cgggcacagc
     9241 aacgagccgc agcgcgccaa cgagcggcgt cgcgtcgaaa ggtaggacag cggtgaccag
     9301 cggtatcgac ttcacatcgg cggcgaactc gttgccgccc aacgcgattg cggtagtcgg
     9361 catggctgga aagtttccgg gggccgactc tgtgtccgca ttctggcgca acctccgcgc
     9421 aggtgtggaa tcgatcgtcg acttgtcgga ggacgagctc atcaccgcag gagtcggcga
     9481 gaaagctctg gccaatcgct cctatgtacg gcgcgccgcg ctcctcccag ggatcgagga
     9541 gttcgacgcc gaattcttcg gcttcacgcc gcaggccgcc cggatgatgg atccgcaaca
     9601 ccggctgttc ctgcagacgg ttttccatgc tttagaggac gccggttatg acccgttgca
     9661 gatcgataac acggtcgggg tcttcggaac gagttccgcc agtgggtatc tgctccacaa
     9721 cctgatgtcg cactacgacc cgaatttggt gatcggacag ggtgcgagct tcgacatggt
     9781 gaacctgtcg ctgcaaaacg ataaggatca tctggcgacc cgggtggctc accagttcaa
     9841 tctgcgaggt ccggcccttg cagtgcagac ggcttgttcg tcgtctctag tcgcggttca
     9901 cttggcctgc cagagcattc tgaatggtga atgcgacatt gcactcgccg gcggctcttc
     9961 ccttcgggtg ccgcaccgtg tcgggtactg gtacgaacag ggatcgatgg tctcgccgac
    10021 ggggcattgc cgaccgttcg acgtcagatc ggatggcacc atcttcggta gcggtgtggg
    10081 tgtcgtcgtc ctcaagccgt tgcaggacgc cgtcgacgac ggcgaccgga tacacgccgt
    10141 gatcagaggt tcggcgctca acaacgacgg ttcgaccaag atgacctacg cggcacccaa
    10201 cgccgttggt caggcagagg ttattgccga ggcgcatgcg gttgcggaca tcgatgcgtc
    10261 gacggtgagc tatgtcgaga cgcatggcac cggcacgccg ctgggggacc cgatcgaaat
    10321 cgagggactg cgccaggcat tcgaggtgtc caccgaaacg aggcccggcc cttgctatgt
    10381 cgggtcggtc aagtcgaaca tcggtcacct cgagaccgca gcaggaatcg ccggtctgat
    10441 caaggcgata ctctgcctgg aacaccgggc gataccggcg acgttgcact acaccagccc
    10501 caaccccgaa ctgcacctcg accgtgggcc attcgtcgtg cgcagcgagg acggcgcatg
    10561 ggagtgggac ggtgttcttc gtgcgggcgt cagctcgttc ggtgtgggtg gcaccaatgc
    10621 gcacgtggtc ctcgaagagc cgccatcagt gccggcagct caaacccggc ccgggccaca
    10681 ggtgttgttg ttgtcggctc gaaccgccga atccctgcaa caatcgcgta cggccttggc
    10741 aacagagctg tccggcgtgg atgaggtgag cctgccggat gtcgcctaca ccctcacgcg
    10801 gcgaaggaaa gaacctgtcc ggctggctgc ggtggtcaat gatcaggagc acgcggcaac
    10861 ggtgcttggg gcaaccgagc acgacaacgt gtttgtgggc gcagccatcc cgcaggccga
    10921 atcacactcg gatcgagtgg ctttcgtttt ccccggccag ggtgcacagc acatcggcat
    10981 ggcacgcggt cttcacgagt gtgagccaat tttcgccgag cagttcgatg cctgcgccgc
    11041 tgccttcggt gacgagatgg gtttcgacct gcgtgcggag gtgttcgacg gagcgggacg
    11101 caacctcgag cgaacggatc gggctcaacc tgctctgttc gcagtggaat acgcgttggc
    11161 gaagctggtc gagtcctacg gcgttcgagc atcagtgctc gccggacaca gcatcggcga
    11221 gtatgtcgca gcaactgtta cgggtgtctt cgaccttccg acggcggtca aggcggtgtc
    11281 gatgcgcgcg cgtctgatgc atgcgtcggc gcgaggtgtc atggtggctg tcgctctgag
    11341 cccggacgcc atcgctgaat acctgggctc agatgtcgat ctcgccgcgg tgaacgatcc
    11401 gggaagctgc gtggtagcgg gatcggaaga gcgcatccgc gatttccagg ctcgtctcgc
    11461 cgagaacggt atcgtggctc gccgggtccg cacggcgcac gcgttccatt cgcggttgat
    11521 ggatccggtg attccggagt tcaacgcctt catgtcccgg ctgacacttc gcgatccgca
    11581 gatcccgttg ctgtcgaaca tcaccggaac gctgatgtcc ggcggtgagg caaccaatcc
    11641 cgcaacgtgg gcccgtcaga tacgggctac ggtacggttc tcagacgaac tcgacgagct
    11701 gcttgccgat ccgacccgtg tactggtgga ggttggtcct ggcggcgctc tgaccggctc
    11761 ggcagtgcgc catccgaagt ggtccgacgg acaccgcgcc gtgcgcctca tgcggcatca
    11821 ggcccagaac aggagcgacc acgacacgtt cctgctagca ctcgggcagt tgtggtcggc
    11881 gggtgtcgag gtcgactggt caccgcggct gactcgccgc tcgcgcttgg tctcgcttcc
    11941 tggttatccc tttgaacgtc aacgacattg ggtccagcac aacgccggcg ccgcgtgggg
    12001 ggcgggtgca accccgacga acgggacggc ggcggcaccg tccgctgcag gcaacacatc
    12061 cgcgcccacc agcagcacat cgcagatgga atcgacgttg cagcgcatct ggtcgcaatg
    12121 cctcggtatc gggtcgatcg accgcaccgc caacttcttc gaactcggcg gtgattcgtt
    12181 gatcgcaatc agcgtcgcga tgacagcctc caaggaggga ctggatctca caccacagga
    12241 cctctacgag aaccagaccg tcgccgcact ggccagggtg ctggttgccc gctacgcaga
    12301 gggcgggctg ggccgccagt cgctgagcga cgcggtgaac ccgccgctcc caccgaacgt
    12361 cgcgcatttc cttgagcatg ggctgcgcga tgtcggtcgc tggcggatcc ccgtgatcct
    12421 gcacctgcgt cccgatgtcg acgtggagga cgtcagatcg gcgctcaccg ccgtgacgaa
    12481 tcatcatgac gcgctgcggt tgcgggtcgt cgagcgagcg ggaacgcggg atcagggcgt
    12541 cgcggaaccc gatgcgttca ccgaactggt cacccgcgca cttcccgaag gtatcgcggc
    12601 cggaagcccg caagagcggg atgcggttct ggcgattctc gacgagcaga ttcgcgagca
    12661 ccaactgatg agcgcgccgt tgaccgccac atacatccgt ggcctgccgg gcggacactc
    12721 gtacctcacg atcagcctgc acgggatcgc gggggacgag acatcacgtg acatcctgct
    12781 cacggacatc ttcaccgcac tcaatcaacg ggttgcgggt caagacatcg tgttacagcc
    12841 cgccacaacg tcgtggcggg agtggtcgca acggtgtgcc gggctcgcta cacaccctgc
    12901 ggtcatggaa acccgcgact actggctggc gacggccacg aaagcgaccc tccgcgtggc
    12961 gggcccggaa gcgtcggaac cgccagggac agacgacctg acgcgtctgt ccgcagcgct
    13021 caccctttcg gaaacgggcg agatcgacga cgcccggcgg cggcttcgcc tgcccgtcga
    13081 agaaatcctg ttggccgcct tgggccgcac ggttgcgggc accgtcggcg agggtgccgt
    13141 cgccgtcgac ctcggtggtc ggggtcggtc cgtactcaag cccgacgtcg acctgcagcg
    13201 aacggtgggc tggttcacca ccatctatcc gattgtcctg cgctgcgcga caactcagga
    13261 ggcgagcgcc aggcagctgc tggacgacgt tcgcgggacc ctgaacgccg tcccgcacta
    13321 cggaattggg tatggccttt tgcggtacat gtacgcgcca acagcacgcc tgcttggcgc
    13381 gacccgggga cccgacattc tgttcacgca tgtgggaaca attcccgacc ttccgtccga
    13441 gcagtccgac gatgccccgg tgcagttcga cgccgatacg gcgttgccgg tgcgcgactc
    13501 cgtgcccggc ctcgggcacg cgatcgagct tcgggtatat cgctcttcgg gtgtgctgca
    13561 cctggattgg tggtacgaca ctcggcggct cggcccgacg gacatggaat cactcgcccg
    13621 gcagttctcg agcgcgctga tggaattgac aagggaagcg ctcgccgaaa atgaaatcga
    13681 ttccggtggt gacgaactgg cccttgtgga cttgtcgtga ccggcaccgg ttaggagacg
    13741 atgatgtcca gcactgagaa ggcggtgatc gtctcaggta tcagcaaaac cttcggttcg
    13801 gttgtggccg ttcgcgatat cagcttcgac atcggggtcg gcgaggtgct cggcttgctg
    13861 ggacctaacg gtgcggggaa gactacgacg gtggacgtcc tgtcgacgct gacccgaccg
    13921 gatcacggtg ttgcggtgat cgcggggcac gatgtcgtca gcgatcccgc cggagtgcgg
    13981 cggtcgatca tgctcaccgg tcagcatgtg tcgctcgacg acatgctgac cggtcgggag
    14041 aacctggtga tgttcggtcg cctgcagggg ctgaagaagg gggaggcgcg atctcgcgcg
    14101 aaggaacttc tcgaacagtt cgacctcgag gatgctggtg accgcaatgt cggtacatac
    14161 tccggtggga tgaagcggcg catcgatata gcgtgcggct tggtggttcg ccccgaagtg
    14221 gtgttcctgg atgaaccgac gactggcctc gatcccagga gccgacaggc gatttgggag
    14281 ctggtgggct cattcaagaa acaggggatc gcgaccctgt tgacgacgca gtacctcgag
    14341 gaggctgatc tgctcagtga ccggatcgtc gtgatcgacc acgggacggt catcgccgag
    14401 ggaacagcag accaactcaa ggaacgcact ggcggaacct actgtgaaat cgttccgtgc
    14461 cacatggacg acatccctgc catggccgag gcgcttggct cgctgctacc agagagcaac
    14521 cgggctgcgc tgaccgacgc atccgaccgc atcgcgatgc cggcgccgga cgggccgacc
    14581 acgcttatgc aggcgctgaa cctggtgaag gaggccaata tcgagctggt tgacatcgct
    14641 ctgcgtcggc cctcgcttga cgaggtattt ctggcgctca cggcacatga cactcgcaat
    14701 acgtccgagc cacgcgtcga agcggaagcg gtctcgtgac cgatacgacc actgccgagc
    14761 tcactgtccg gccaacggca tccaccatcc agcagtggtg ggtgctgacg gttcggatgc
    14821 tcacgccgac gctgcgcaat ggggagctgg ccacccagat cgtaggatcc atcgtcttca
    14881 ccatcggcta ctacataccg ctcaagcaga tcatgggtgc ggcgcagggc atgagtagct
    14941 atgcgcagta cctgacgccg ctgattgtcc ttcaagcgat ctggttcgct gcggtgtcgg
    15001 cagcgttccg atcggcaaca gactcggttc agggcatcaa ccggcggttc aaagccatgc
    15061 cgatcgcctc attgacccca ctcgcatctc gcatgtcggc cagcatgtac cggtgctgcg
    15121 tcgcattgac ggtctctgtg gcgtgtggcc acgtcattgg atttcggttt cacgacggcg
    15181 tggtgcacgc cgtcggcttc tgtgtgttgg tactactgat cggtgcggcg ctggcgttca
    15241 tcggagactt gatcggtgtt gcgacgcaga atcccgaggc gaccgcgccg ttgatgctgg
    15301 tgccgcaggt tacgctcggc ctggcatcga ccggcctgca accggtcgaa cggtttcccg
    15361 agtggatcca accgtttgtt cggaaccaac cgctgtccca gttcgtctac gcgttgcagg
    15421 cattggccgg ggacagcaca tcagcagccc cagaggtgcg gtggtccgtc atcggcccgg
    15481 cactggcctg ggtggccggc atgatcctga tctccgtcgg gctgcacatg cgggtttcca
    15541 aaaggcggcc ctgatgtctg tcgcatcgca ggacaagaag accatcgccg tcggtcgagc
    15601 ggcaggcaca caggtcgaaa cgcgtactca ccccgctcca ggccggcacc ggctgccccg
    15661 gccccgggag aactcgcctc ggcgcttgat tccgcacact tgggcgctca cggcgcgaat
    15721 acttcgccgg tggagccgcg atcccgcaac ggtggtgcaa tcgctggtct ttcccgccgg
    15781 attcctagtg gcacttgaca tcgtccttgg cgacggtgtg gagcaggtca ccgggcacag
    15841 cgccctctat ggcagtgtcc cgctggtcgc aacggtcggc gccacgtccg gtgcgatcat
    15901 cggtgcggtc ggcctcatgc gtgagcgtag cgacggcttg ttgtcccggc tttgggtgct
    15961 acccatccat cgcgcatcgg gcgtgatggc caggctgacg gctgatgcca tccggatcgt
    16021 ggtgatcacc acggtcgtgc tgggtgtggg gctgatgctc ggtttccggt tcaagcaagg
    16081 gatcggtgaa gcagcgctgt ggctgttcat ccctgctttg ttcggagtcg ctttctcgtc
    16141 ggccgtcatc acgctcgcgc tgtattcgtc caacaccatc gtgccgcagg cgaccgaaat
    16201 catctgcgca atgctgttct tcttcagtac ggggttcgtt ccgctcgacc aatttccgag
    16261 ctggatacaa ccgatagtcg agcaccagcc gatcagttat gtcatcgaga cgatgaaggg
    16321 cttgtctctc ggcggcccgg tgttggcacc gctagtcgca acgttgctct ggtcggcggg
    16381 tatcgccgcc ctatgcgccg taccaatggc gatcgggtac cgaagggcca gcaagcgtgg
    16441 atgatcgccg agtcgtgttc acccactggc agggagtctg agagtgtttc cttcatcagt
    16501 tcttcgtaag ctggcgcgca gcgaggaaat gttcgcggag acacacaatt tcgttggtct
    16561 cggcgcgcac gtggaaggcc ccgtcgacgt tgacgcgttg tctgatgcgt tcgacgcgct
    16621 gcttcaggca catccggcgc tcgcaggcca cctcgaacag gacccagacg gccgttacca
    16681 gatcgtcgtc gacgaccttc tccatcccgg aatagaggtg atcgagctcg acgatccggc
    16741 agccaaaacg ccgccactgt acttcgacca aaccgcatca ctggctcatt tccggctgac
    16801 gatccgcgat ggacaagcac aaccgacgct ctacatccat cacagcctcg ccgacggcca
    16861 ccatcagttc agcctcgtcg aagaattgtt ttcctactac acagatctcg tcacaacggg
    16921 aacgacgcgt ccgctcgacg tgcaccccgt gccggacccg ttggaggtcg tgttggccga
    16981 ccgcggtgtg cagaaagtaa accggtcagg tctcgagcgg ttgttgacgg cgatgttcgt
    17041 ctatgacttg ccgccatccc ggcgcacggc gtctgaggtc aaacccgcgt ggccgcagct
    17101 cgttccgatg gcgttctgtc gcctaagcga gcgcgagacc gacgacctag tcgcgttctg
    17161 ccgggtgcac aaggttggtt tgaacggtct actctctgcg gtggtgctaa tggcggagtg
    17221 gcgacttcgc gcaacgccga agatcccggt tccctatgtc tatcccgtcg atcttcggta
    17281 cgtgctgtca ccaccggtgt ccgcgaccgc gtgcaccaac ccggtcggga tagccaccta
    17341 tctcgccgag atcgaccaca acacggatgt cgtcgacctc gcccgcgaca tcgccgacac
    17401 ttttcgaaaa gatctgtcgg aaggggtgat tcagcagtcc ttccttcact tcagccctca
    17461 gtacgtcggc aatccgcccg ggcttcccga cgtcgtcatg ttcaccgaca acggggtggt
    17521 cccaccaatc cgtacgcccc ctgaaatgaa aatcaccgca agccacggtg agttctactt
    17581 cggtgtcagc gccggaatcg agatctacac cagcaagatt ttcgccggcc aactgatgat
    17641 cgaatatcat tcacacgggc cggaaccgga gaagtc
//
LOCUS       DASRJA010000667         7847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_88612,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000667 DASRJA010000000
VERSION     DASRJA010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88612"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="VFC01_29875"
     CDS             complement(<1..1526)
                     /locus_tag="VFC01_29875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZN83858.1"
                     /translation="MLEGCILAGSRQKSAVATTNSSVPGAPNRISFAKLREPLEVPGL
                     LDVQTESFEWLIGSERWRNNASLRGDAKPVGGLEEVLSELSPIEDFSGSMSLSFSDPR
                     FDEVKAPVDECKDKDMTYAAPLFVTAEFINNNTGEIKSQTVFMGDFPMMTEKGTFIIN
                     GTERVVVSQLVRSPGVYFDETIDKSTEKTLHSVKVIPGRGAWLEFDVDKRDTVGVRID
                     RKRRQPVTVLLKALGWTNEQITERFGFSEIMMSTLEKDNTAGTDEALLDIYRKLRPGE
                     PPTKESAQTLLENLFFKEKRYDLARVGRYKVNKKLGLHAGEPITTSTLTEEDVVATIE
                     YLVRLHQGDKAMTVPGGVEVPVEVDDIDHFGNRRLRTVGELIQNQIRVGLSRMERVVR
                     ERMTTQDVEAITPQTLINIRPVVAAIKEFFGTSQLSQFMDQNNPLSGLTHKRRLSALG
                     PGGLSRERAGLEVRDVHSSHYGRMCPIETPEGPNIGLIGSLSVYARVNPFGFIETPYR
                     "
     gene            2108..2323
                     /locus_tag="VFC01_29880"
     CDS             2108..2323
                     /locus_tag="VFC01_29880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83859.1"
                     /translation="MCGVRLDSRERECGLDWGLLGFRRGDGFVDGPGLVGGRQRGFLG
                     LRGHICVVGLDNGVNGVLGVVVVDGLT"
     gene            complement(2338..2703)
                     /gene="frdD"
                     /locus_tag="VFC01_29885"
     CDS             complement(2338..2703)
                     /gene="frdD"
                     /locus_tag="VFC01_29885"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013807753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HZN83860.1"
                     /translation="MSTSAPHRRSSEPFLWLLFSAGGMAAALVLPVLLFLFGVAFPLG
                     ILDAPDHAHLLAVVQNPLTKLVLFGVSVLALFHWAHRFRFTLEHGLQLGRFDRVIALC
                     CYGTAVLGSAAAGWVLLTA"
     gene            complement(2700..3080)
                     /locus_tag="VFC01_29890"
     CDS             complement(2700..3080)
                     /locus_tag="VFC01_29890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015627497.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; FrdAB are the catalytic
                     subcomplex consisting of a flavoprotein subunit and an
                     iron-sulfur subunit, respectively; the catalytic subunits
                     are similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HZN83861.1"
                     /translation="MHAYRQPISRFWWAKRRSYLLFMLREISCAFVAWFVVYLLLLVH
                     ATSAGPDSYARFLEWSANPLVVAVNAVALIFLLLHAVTWFGLAPRAIVPHIRGRRVPA
                     RAVLAGHYAAWLVVSAVLAWVVLS"
     gene            complement(3089..3832)
                     /locus_tag="VFC01_29895"
     CDS             complement(3089..3832)
                     /locus_tag="VFC01_29895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HZN83862.1"
                     /translation="MADRIVMEVTRFRPETDSEPVFEAYEVPLTREWAILDGLNYIKD
                     QLDGTLTFRWSCRMGICGSCGMTINGDPKLGCATFLADYLPGPVRVEPMRNFPVIRDL
                     VVDISDFMAKLPRVKPWIIRDDELSVDDGEYLQTPAELDEFKQFSMCINCMLCYAACP
                     VYALDPEFLGPAAIALGQRYNLDSRDEGAADRSDVLAAAEGAWACTLVGECSTACPKG
                     VDPAGAIQRLKLTAATHSVKELLFPRGAR"
     gene            complement(3835..5574)
                     /gene="frdA"
                     /locus_tag="VFC01_29900"
     CDS             complement(3835..5574)
                     /gene="frdA"
                     /locus_tag="VFC01_29900"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HZN83863.1"
                     /translation="MTASYNIVIVGGGGAGLRAAIAIAEMNPQLSIAIVSKVYPMRSH
                     TVSAEGGAAAVAGEDDSLDEHAYDTVSGSDWLCDQDAVEAFVAEAPRELLQLEHWGCP
                     WSRKPDGHIAVRAFGGMKKLRTWFAADKTGFHLLHTLFQRVLAYPDIVRYDEWFATTL
                     LVDDGRVRGLVAVELSTGRIETILADAVIVCTGGCGRVFPFTTNGNIKTGDGMALAFR
                     AGAPLKDMEFVQYHPTGLPFTGILITEAARAEGGWLLNKDGYRYLQDYDLGKPTPEPK
                     LRSMELGPRDRVSQAFVHEQEKGRTVETPYGHVVYLDLRHLGAELIDTKLPFVRELCC
                     NYQHIDPVTELVPVRPVVHYMMGGVHTDIHGATPISGLYAAGETACVSINGANRLGSN
                     SLPELLVFGARAGHAAAEYAATVGGASSAVQAQARSEEQRLEHELSRRGDGNERIADI
                     RTEMQATLETAAGIYRDGPSLTKAVDEIRVLQERFARVGIDDHSHTFNTELTALLELS
                     GMLDVAQTIVESALHREESRGAHQRTDFPKRDDEHFLAHTLVHREPDGTARIDHLPVT
                     ITRWPPGERVYGR"
     gene            complement(5584..5826)
                     /locus_tag="VFC01_29905"
     CDS             complement(5584..5826)
                     /locus_tag="VFC01_29905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003407763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83864.1"
                     /translation="MAATTTHFPKQKAPCGRMAEGDRHLESDDDGLAFDDLKYTCGCR
                     ETRHVYHDGCVRIRTIRHDGKVLRDERCGEHEAFEV"
     gene            6077..>7847
                     /locus_tag="VFC01_29910"
     CDS             6077..>7847
                     /locus_tag="VFC01_29910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016882611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN83865.1"
                     /translation="MASSDPLPTQADVHAGVPAELVAAGFEDPEEIGRGGFGVVYRCS
                     QRELDRVVAVKVLTADLEPDNLERFVREQVAMGKLSGHPNIVAIFQVGTTATGRPFIV
                     MQYHPHGALEAKIHHNGPVGWADALHIGVKVAGALETAHRRGTLHRDVKPANILLTEY
                     GEPQLTDFGIARIIGGFETGDGAVMGSPAYTAPEVLLGESPDATSDVYSLASTLFCAG
                     TGHAVFERRKGEQMVAQFLRITKHPMPSLRDSGLPTDVCAVIEQAMSRDIEDRPATAE
                     AFGEQLRDVQRMHGLPVDDMAVPIPAPTIRYNPPRSSGTPSGSYPARTLTPPVPATRF
                     QPPVSTKALVDRARLIDVLRAGHGKKLTVIHGPTGFGKSTLAAQWAKLLTAEGVAVAW
                     LTVDHDDNNVVWFLAHLIEAIRAVTPALATELGEVLQEHDDEAERYVLTSLINEIHET
                     GTRMTLVIDDWQRVTDPATIGALLYLLVYASDDLTVVVTSRNQSGLPMSRMRMLDQLL
                     EIDATALRFDVAESENFLVDLGGLDLDHADVEELTAKTDGWVAALQLASLSLRGRDDP
                     VPLIDTMTGRHHAISAFLAENVLD"
BASE COUNT         1414 a         2525 c         2492 g         1416 t
ORIGIN      
        1 ttgcggtagg gcgtctcgat gaagccgaac gggttcaccc gcgcgtacac cgacagcgag
       61 ccgatcagac cgatgttcgg gccctcaggg gtctcgatcg ggcacatccg gccgtagtgg
      121 ctggagtgga cgtcgcggac ctcgaggccg gcgcgctcac gggacagacc gccagggccc
      181 agcgccgaca ggcggcgttt gtgggtcaga cccgacagcg ggttgttctg gtccatgaac
      241 tgcgacagct ggctggtgcc gaagaactcc ttgatcgccg ccacgacggg acggatgttg
      301 atcagggtct gcggcgtgat cgcctcgacg tcctgagtgg tcatgcgctc gcgcacgacg
      361 cgctccatgc gggacaggcc gacccggatc tggttctgga tcagctcgcc gacagtgcgc
      421 aggcgacggt tgccgaagtg gtcgatgtca tcaacctcga ccggaacctc gacaccgccg
      481 ggaaccgtca tcgccttgtc gccctgatgc aggcgcacca ggtactcgat ggtggcgacg
      541 acgtcctctt cggtcagcgt ggaggtggtg atcggctcgc cggcgtgcag gccgagcttc
      601 ttgttcacct tgtagcgacc cacgcgggcc aggtcgtagc gcttctcctt gaagaacaag
      661 ttctccagca gcgtctgcgc ggactccttg gtcggcggct cgcccggacg cagcttgcgg
      721 tagatgtcga gcagcgcctc gtcggtgccc gcggtgttgt ccttctccag cgtgctcatc
      781 atgatttcgg agaagccgaa gcgctcggtg atctgctcgt tggtccagcc gagcgccttg
      841 agcagcacgg tgacgggctg acggcgcttg cggtcgatgc gcacgccgac ggtgtcgcgc
      901 ttgtcgacgt cgaactccag ccacgcgccg cggccgggga tcaccttgac gctgtgcagc
      961 gtcttctcgg tggacttgtc gatggtttcg tcgaagtaca caccgggcga gcggaccagc
     1021 tgggacacca cgacacgctc ggtgccgttg atgatgaagg tgcccttctc ggtcatcatc
     1081 gggaagtcgc ccatgaagac cgtctggctc ttgatctcac cggtgttgtt gttgatgaac
     1141 tcggccgtga cgaacagcgg ggccgcgtac gtcatgtcct tgtctttgca ctcgtcgacg
     1201 ggggccttta cctcgtcgaa gcgcggatcg gaaaagctca gcgacatcga gccggagaag
     1261 tcctcgatcg gcgagagctc cgagagcacc tcttcgaggc cgcccaccgg cttcgcatcg
     1321 ccacgaaggc tggcgttatt ccgccagcgt tccgagccga tcagccattc gaacgactcg
     1381 gtctgaacgt caagtagccc cggaacctcg agaggctcac ggagcttggc aaatgaaatt
     1441 cggttgggtg ctccgggaac ggagctatta gtggtagcta ctgcggactt ttggcgggac
     1501 cctgccaaga tgcatccttc cagcacctca tgcgaccgtt cggatgccgg ttacaccgga
     1561 cctgtagtcg ctatctgcgt cggttcggct ggcctctcaa gaccacaaac ttcccgaacg
     1621 cacggcacgc gactagatca ctgagcgggg ctcaggctag gacatcggtg tcaggtgcgg
     1681 gtttaggtgg gcaggatgca gccagcgcaa cgtccaacag taccgcagga cggcgtattc
     1741 ctcaaccact gcatgcagaa gtcaccgggc gctggctggc gtcctcaacc cgtgcataca
     1801 tgctgcccaa cagactggcc cgtcgaaccc ttcccgtcaa gagataacac ggtgtttggt
     1861 gtggagtttt ccgttaattc tgggtttgcc tgcagcagcg gcgaggcgct tgggtccgcc
     1921 gcacagctgg cgaagttgaa ggccgccggc aactccagaa aggtccgcag gacaaggttc
     1981 aaccaaggtg agctgggagt cggcccgggc agaacgtagc cgtgcgcgcc acccgcgtcg
     2041 gtcgggacgt cgatgacgac ctcgcccggc tcgccgaagt cgatgttgag gagcgtcggc
     2101 cccgtgggtg tgtggcgtcc ggctcgacag ccgcgaacgc gagtgtggtc tcgactgggg
     2161 tctgcttggc tttcgccgcg gcgacggctt cgtcgacggg cccggccttg ttggcgggcg
     2221 gcaacgtggc ttcctcggtc tgcgcgggca catctgcgtc gttggtctcg acaacggcgt
     2281 caacggcgtc cttggcgtcg tcgttgtcga cggcctaact tgatgccgcg gccggcgcta
     2341 cgcggtgagc aatacccaac cggccgctgc ggatcccaat actgctgtgc cgtaacagca
     2401 cagggcgatc acacggtcga accggcccag ctgcagccca tgctcgagcg tgaatcggaa
     2461 gcggtgcgcc cagtgaaaca gggcaagcac agagacgccg aatagcacaa gtttggtcag
     2521 cggattctgt acgactgcta gcaggtgcgc atggtcgggc gcgtcgagga tccccagcgg
     2581 gaacgccacc ccgaaaagaa acaacagcac gggcagcacg agcgcggccg ccataccccc
     2641 ggcgctgaac aggagccaca ggaatggctc agatgaccga cgatgcgggg cggatgtgct
     2701 catgacaaga ctacccacgc gagaaccgcc gatacgacga gccacgccgc gtaatgcccc
     2761 gcaagaaccg cgcgagcagg aacgcggcgt cctcgtatgt ggggcacaat cgctcgcggc
     2821 gccaaaccga accatgtaac agcgtgcagc agaaggaaaa tcaatgcgac ggcgttgacc
     2881 gcaaccacga gcggattcgc actccactcc aggaatcgtg cgtacgaatc cggtcccgcg
     2941 ctagtcgcat gcaccaagag cagcaggtac accacaaacc aggccacgaa ggcgcaactt
     3001 atttcgcgaa gcatgaacag caggtaagac cgccgcttcg cccaccagaa cctgctgatg
     3061 ggttgacgat aagcgtgaat cgacgtcgtc atcgtgcgcc ccgtgggaag agcaattcct
     3121 tcaccgaatg tgttgccgcg gtcagcttga gacgctgtat cgcacccgcc ggatcgactc
     3181 ccttcggaca agccgtcgaa cattcaccca ccaacgtgca cgcccacgcg ccctcggccg
     3241 cggcgagcac gtcgctccgg tccgctgcgc cttcgtcgcg cgagtccagg ttgtaacgct
     3301 gacccagagc gatcgccgct ggaccgagaa actccgggtc cagcgcgtac accgggcatg
     3361 ccgcgtagca caacatgcag ttgatgcaca tgctgaactg cttgaattcg tccaattcgg
     3421 ctggggtctg caagtattcg ccatcatcga cggacagttc gtcgtcacga atgatccagg
     3481 gcttgactcg cggcagtttg gccatgaagt cactgatatc gacgaccaga tcgcggatca
     3541 ctgggaagtt tcgcatcggc tccacccgca ccggcccggg caggtaatcg gcgaggaacg
     3601 tcgcgcaccc caatttcggg tctccgttga tcgtcatgcc gcagctgccg cagataccca
     3661 tccggcacga ccatcggaag gtcagcgttc cgtcgagctg gtccttgatg tagttcagac
     3721 cgtcgaggat cgcccattca cgggtgaggg gaacttcgta ggcttcgaaa accggctcgg
     3781 aatcggtttc cggccgaaac cttgtgacct ccatgacaat tcggtccgcc atcgctacct
     3841 cccatacaca cgttcgcccg gcggccagcg cgtgatcgtg accgggaggt gatcgatccg
     3901 cgccgtcccg tcgggttctc ggtgaaccaa cgtgtgggcc aagaagtgtt cgtcgtcgcg
     3961 cttggggaag tcggttcgct ggtgcgcgcc acgcgattct tcccggtgca aggcagattc
     4021 gacgatcgtc tgtgcaacgt cgagcatccc cgacaactcg agcagcgccg tcagctcggt
     4081 gttgaacgtg tggctgtgat cgtcgatgcc gaccctcgcg aatcgctcct gcaacactcg
     4141 gatctcgtcg accgccttgg tgaggctggg tccgtcacga tagatcccgg cggctgtctc
     4201 caacgtcgcc tgcatctccg tgcgaatgtc agctatgcgt tcattgccgt cgccacggcg
     4261 agagagctca tgctccagcc gctgctcctc gctgcgtgcc tgagcctgca ccgccgacga
     4321 ggcgccgccg accgtcgccg cgtactccgc cgcggcgtgg ccggcgcgcg ctccgaacac
     4381 cagcagctcg ggaagcgagt tcgaccccag ccggttggcg ccattgatgc tgacgcacgc
     4441 cgtctcgccc gccgcgtaga gcccggaaat cggcgtagcg ccatggatat ccgtgtgaac
     4501 gccgcccatc atgtagtgca ccacgggccg aacggggacc agctcggtca ccgggtcgat
     4561 gtgctggtag ttgcagcaca gttcacgcac gaacggcaac ttggtatcga tcagctcggc
     4621 gccaaggtgt cgcaggtcaa ggtagacgac gtgtccgtag ggggtctcga ccgtccgtcc
     4681 cttttcctgc tcgtgcacga acgcctgaga cacccggtct cggggtccga gctccatgct
     4741 gcgcagcttc ggctcgggcg tgggtttgcc gaggtcgtag tcctgcaggt agcggtagcc
     4801 gtccttgttc agcaaccaac caccctccgc tcgcgctgcc tcggtgatca agattccggt
     4861 gaacggcaat ccggtggggt ggtactggac gaactccatg tccttcagcg gcgcgcccgc
     4921 cctgaacgcc agggccatcc cgtcccccgt cttgatattc ccgttggtgg tgaacggaaa
     4981 cacgcgccca catccgccgg tgcagacgat caccgcgtcg gcgaggattg tctcgatgcg
     5041 ccccgtcgag agctcgacgg cgacaagacc gcgcacccgg ccgtcgtcga cgagcagggt
     5101 cgtcgcgaac cactcgtcgt aacgcacgat gtccggatat gcgagaacgc gctgaaacag
     5161 tgtgtgcagc aggtgaaatc cggtcttgtc ggcagcaaac cacgtacgta gcttcttcat
     5221 accgccgaat gctcgaactg caatgtgccc gtccggctta cgactccacg gacaacccca
     5281 atgttcgagt tgcaacaact cacgcggggc ctcggccacg aaggcttcaa cggcgtcttg
     5341 gtcgcacagc cagtcgctcc cggaaacggt gtcgtatgcg tgttcgtcga ggctgtcgtc
     5401 ttcgcccgct accgccgctg caccgccttc tgcggagacg gtgtggctgc gcatcggata
     5461 caccttggag acgatcgcaa tgctcagctg cggattcatt tcggctatcg ctatcgcggc
     5521 gcgcagcccc gcgccacccc caccgactat cactatgttg tacgacgccg tcatcgcagt
     5581 ggcttacacc tcgaaggctt cgtgctcgcc gcagcgctcg tccctcaaca ccttgccgtc
     5641 atgccttatc gtccggatgc gcacgcatcc gtcgtggtag acatgccggg tctcgcggca
     5701 gccgcaggtg tatttgaggt cgtcgaaggc cagcccgtcg tcgtcacttt cgagatgacg
     5761 atcgccctcg gccatccgcc cgcagggtgc cttctgcttg gggaaatgcg tggtcgtcgc
     5821 ggccatcgtc ccgcctcctc agatgtcggg cgagtgatct agatcacatc aggatatgcc
     5881 tggacaagag tgcgcgcggg taatcgtcat gaccggcttg tcagggttcg tcggcgccgt
     5941 tgggctcggc cggagcgtaa gccgtttccc gcaatcgatc ggggccattc atccgccgtc
     6001 gaccggcgaa cgttcggttt atgctcaggt caggtgccat ggcgcggcca tggcgccagc
     6061 gatatcgagg cgaccgatgg ccagcagcga cccgctgcct acgcaggcgg atgtgcacgc
     6121 cggggtcccg gcggagctgg tagctgccgg ttttgaggat cccgaggaaa tcggccgcgg
     6181 cgggttcggc gtcgtatatc gctgcagcca acgcgagctg gaccgcgtcg tcgcggtaaa
     6241 ggtgctaacc gcggatctcg aacccgacaa cctggagcgc ttcgtacgtg aacaggtggc
     6301 gatgggcaag ttgtccggac acccaaacat cgtcgccatc ttccaggtcg gaacaaccgc
     6361 caccggcagg ccattcatcg tgatgcagta ccacccgcac ggtgcgttgg aggccaagat
     6421 ccaccacaac ggaccggtgg gctgggccga cgcgctgcac atcggcgtga aggtcgcggg
     6481 cgcgttggaa accgcacacc gacgtgggac gctgcaccgc gacgtcaagc ccgccaacat
     6541 cctgctcacc gaatacggcg aaccgcagct gaccgacttc gggatcgccc gcatcatcgg
     6601 tgggttcgag accggcgacg gcgcggtcat gggttcgccg gcatataccg ccccggaggt
     6661 actgctcggc gagtcaccgg acgcgacctc ggatgtctac agtttggcgt cgacgctgtt
     6721 ctgcgccggc accggccacg cggttttcga gcgccgcaag ggtgagcaga tggtcgctca
     6781 gtttctccgg ataaccaagc accccatgcc gagcctgcga gactcgggcc tgcccaccga
     6841 cgtatgcgcg gtgatcgagc aggcgatgtc gcgcgatatc gaggatcggc cggcgaccgc
     6901 ggaggcgttc ggcgagcagt tgcgcgacgt gcagcggatg cacggcttgc cggtggacga
     6961 catggcagtg cccataccgg caccgacgat ccggtacaac ccaccgcggt cgtccggcac
     7021 gccatcgggt tcatatccgg cacggacgtt gacgccgcct gtaccggcga cccggttcca
     7081 gccgccggta tcgacgaagg cgttggttga ccgggcgcgg ctgatcgacg tcctacgcgc
     7141 cggacacggc aagaagctca cggtgattca cggcccaact ggctttggga agagcaccct
     7201 tgccgcccag tgggcgaagc tgctgaccgc cgagggcgtc gcggtggcat ggctgaccgt
     7261 tgaccatgac gacaacaacg tcgtctggtt tctggcccac ctgatcgagg ccatccgtgc
     7321 ggtcacgcct gcgctggcga ccgagctcgg cgaagtgttg caggagcacg acgacgaggc
     7381 cgagcgttac gtcctgacct cgttgatcaa cgagatccat gagacgggca ctcgcatgac
     7441 gctggtgatc gatgactggc agcgcgtcac cgacccggcg accatcggcg cgctgctgta
     7501 cctactcgta tacgcctctg atgatttgac cgtggttgtg accagccgca accagagcgg
     7561 cctgccgatg agccggatgc ggatgctcga ccaacttctt gaaatcgacg ccaccgcatt
     7621 gcgtttcgat gtggctgaat cggagaactt tctggtcgac ctgggtgggc tggacctgga
     7681 ccatgcggac gtcgaggagc tcaccgccaa gactgacggc tgggtggccg cactgcagct
     7741 agcatcgctg tcgctgcgcg gccgcgacga tcccgtcccg ctgatcgaca cgatgaccgg
     7801 ccgccaccac gcgatcagcg cgtttctggc cgagaacgtg ctcgaca
//
LOCUS       DASRJA010000668         6794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2055227,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000668 DASRJA010000000
VERSION     DASRJA010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2055227"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1660)
                     /locus_tag="VFC01_29915"
     CDS             complement(<1..1660)
                     /locus_tag="VFC01_29915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase family protein"
                     /protein_id="HZN83866.1"
                     /translation="MTKIYNDPADFSGEALSGFAGAFPKYVQQVPGGLVRAGPPSPGK
                     VAVVIGGGTGHYPAFAGWVGPGMADGAVVGDVFASPSTQQIVSVARAADAGGGIFLSY
                     GNYAGDVLNFDAARDQLIAAGVPTRSVVVTDDLASAPPEDRPLRRGTAGDLIVIKIAS
                     AAAESGYDLDGVAAVADRVNKRTASLSVAFSGCTPPGSGAPLFTLAPGQMAVGLGLHG
                     EPGISEQPILSAADLAELLVERVLVERPENATQAAVLVNGLGATRYEELFVLWHEIAP
                     RLEAADVEVIDPEVGELVTSLDMAGASLTIGWLDDETCALWSAPANTPAYRKITMEPV
                     PAARPIQPAVARAIPAASEASRLIAKSIVAALESAAESLDQHEADLNQLDAVAGNGDH
                     GRVMVRGSRAAAAAAQRAVAEDAGAATTLLLAADAWSDRAGGTSGALWGAALRAAARV
                     MSDDTVSPHTATTALRAALDAITAYGNARLGDKTLVDAFLPFVESFEQDMQAGLPLGA
                     AWVNAADAAARAADATASLTPRLGRARPLAARSLGHIDAGARSFA"
     gene            2230..2538
                     /locus_tag="VFC01_29920"
     CDS             2230..2538
                     /locus_tag="VFC01_29920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83867.1"
                     /translation="MKKIIFTSLAAGALASATLGLAGAASAAPSGPPPVDDTISQLQA
                     KGFNVLVSRVDSDGVDRCTLNAVRPGQTFSRTDSGVPGAQDDLVTTVTDKTVFVDVVC
                     "
     gene            complement(2617..3897)
                     /locus_tag="VFC01_29925"
     CDS             complement(2617..3897)
                     /locus_tag="VFC01_29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HZN83868.1"
                     /translation="MSSNINRRLFLTRAGLVMGGAVAGPSFIAACGGGQGGGGATDKF
                     KVGAVLELSGESATGGQIAQRGYQLWADTVNKAGGLSIGDKKYEVDLIVQDCKSDPAT
                     GADATSRLVSEEGVNAIFGAYTSGVQLAMDPICAKYRVPCIAGSAESPNVWKGKPEFT
                     FGVIPAVDTTAARSIQAIVDNANPKPATAAVVGANEPFSDNTAEGFRTGVQDAKLNLV
                     HYSLFPPNADLAPVAGVVAAQRPDIVAVGGHDVLLVDFVKAMAATGYTPKAIIEHYGI
                     TDASFAQALGRQADGVMGISVWLPTASFKDDLFGSAGDYAKTFEEQHGSPPDYTAAGC
                     SAAGQVLQAAVEKLGQPPSLSEEARVKLNELIAGTDLQTFYGPIKFASEGDHFHNNTA
                     LSPMLVQIQGGQVKAIAPPDAAEAKIIYPLAPLG"
     gene            complement(3949..4692)
                     /locus_tag="VFC01_29930"
     CDS             complement(3949..4692)
                     /locus_tag="VFC01_29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN83869.1"
                     /translation="MSERQGSDSRDGVVTVEGLDAGYGAVQILHQVSMTARTGEVTCI
                     FGPNGCGKSTLLKAMVGVIDPWAGKITIDDDDLTHLPSHRTLGRGLAMMPQGGGVFPQ
                     LSVRDNLRIGGYTIRSRKQLDQRIDELLEEFPRLRERYDVQAGQLSGGEQMILSIARA
                     LILRPRFLLFDEPSAGLSPKLVGDVLVRAAELAKRGVGVIMIEQNIREALRVADRMYV
                     LVAGRNRFEGTPADVEDDRELMHLYLGGR"
     gene            complement(4685..5401)
                     /locus_tag="VFC01_29935"
     CDS             complement(4685..5401)
                     /locus_tag="VFC01_29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN83870.1"
                     /translation="MTVLAVDDLHRSFGGIRAVDGVTFGVEQAEIVGIIGPNGSGKST
                     LFNLLTGALKPDGGSISLFGQNVTRLAPYKIARTGLSRTFQIPALFVNMTVRENLWTA
                     AVQFDWDNARANADAVLERLELTHVADDLASTLSGGQQRLLEMGRVLMQKPKVALLDE
                     VAAGVHPRLRQIMLDAIRTLRDAGTTFLIIEHDMELAQDICDRIIVMDAGKIVAQGSF
                     EEISHDPHVMEAYLGVSADE"
     gene            complement(5398..6423)
                     /locus_tag="VFC01_29940"
     CDS             complement(5398..6423)
                     /locus_tag="VFC01_29940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN83871.1"
                     /translation="MSRPASGGNPYLNLGVLALVVAALCLLPMMTTPYYIRVATGIAL
                     WAGIALSWNVICGYAGYISFGHVAFFGIGAYTTAILMQPNRGWNFWATLPVAAVIAGA
                     VAALVGWPALRLKGAYFAIATWALAEAVREFTTVVDFTGGSGGLKTPIRLDDNFFFYT
                     MLGAAAIAYAMCYLLLERSRFGFQVKAVRDNESAARAQGINTTLVKIEAFVLSAMIPA
                     VLGGINAYWITFINPASVLNTIITDQLVVMVLVGGLGHAWGPALGATAMFLLQEQLRV
                     SYGETTAYIIVVGLLVMAIVLFLPDGLVSLGQRARRMRLVRQLLGRVRDDVAPRRTEN
                     QTEEAIQ"
     gene            complement(6485..>6794)
                     /locus_tag="VFC01_29945"
     CDS             complement(6485..>6794)
                     /locus_tag="VFC01_29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN83872.1"
                     /translation="RISSLVYAIYTGITAIAGAMLGAIYAMTPEVGLRYTLFAFFVVV
                     LAGLGSVVGVMAAGLFLGVLQSLVTTYVGADYTLLVVFGVLFVALLLFPQGISRRGLA
                     "
BASE COUNT         1143 a         2163 c         2363 g         1125 t
ORIGIN      
        1 tggcgaacga cctcgcgccg gcgtcgatat ggccaaggct gcgtgcggcc agggggcggg
       61 cgcggccgag tcgaggggtc agcgacgcgg tcgcgtcggc ggcgcgggcg gcggcgtcgg
      121 cggcgttcac ccacgcggcg ccgaggggta gtccggcctg catgtcttgt tcgaagctct
      181 cgacgaaggg caggaacgca tcgacgaggg tcttgtcgcc gagccgcgcg ttgccgtagg
      241 cggtgatcgc gtcgagggcg gcgcgaaggg cggtggtcgc agtgtggggc gaaacggtgt
      301 cgtcggacat cacgcgggcg gcggcgcgca aggctgcgcc ccacagcgcg ccggaggtcc
      361 caccggcgcg gtcactccac gcgtcggcgg cgagcagcag cgtggtggcg gcaccggcgt
      421 cctcggcgac ggctcgttga gcggcggcgg ctgcggcgcg cgaaccgcgg accatcaccc
      481 ggccgtggtc gccgttgccg gcgacggcgt ccagctggtt gaggtccgcc tcgtgctggt
      541 cgagtgactc ggctgccgat tccagcgctg ccacaatgct tttggcgatg agtcgagatg
      601 cctcggacgc tgccggaatg gcccgggcga cggctggctg gatcggtcgg gccgccggga
      661 ccggctccat cgtgattttg cggtaggcgg gtgtgttggc aggagcggac cacagtgcgc
      721 aggtttcgtc atcaagccag ccgatggtca gcgatgcgcc cgccatgtcg agactggtga
      781 cgagttcgcc gacttccggg tcgatcacct cgacgtcggc ggcttcgagc cggggtgcga
      841 tctcgtgcca gagcacaaag agttcctcgt agcgggtggc gccaaggccg ttgacgagaa
      901 cggctgcctg cgtagcgttt tcgggtcgct cgacgaggac gcgctcgaca agaagctcgg
      961 cgaggtcggc ggcgctgagg atgggctgct cggagattcc gggctcgccg tgcaggccga
     1021 ggccgacggc catctggccg ggtgcgagcg tgaacagggg cgccccggag ccggggggtg
     1081 tgcagccgga gaaagcgacg ctgagggaag cggtgcgttt gttgacgcgg tctgccaccg
     1141 cggcgacgcc gtcgaggtcg tacccggatt cagcggcggc cgaggcgatc ttgatgacga
     1201 tgaggtcgcc ggcggtgccg cgtcttagcg gccggtcttc gggtggggcg gaggcgaggt
     1261 cgtcggtgac gacgacggat cgagtgggca cgcctgcggc gatgagttgg tcgcgggcgg
     1321 cgtcgaagtt gagcacatcg ccggcgtagt tgccgtagct gaggaagatg ccgccgccgg
     1381 cgtcggcggc gcgggcgacc gagacgattt gttgggtaga gggggaggcg aagacgtcgc
     1441 cgacgaccgc cccgtcggcc atgccgggac cgacccagcc ggcgaaggcg ggatagtggc
     1501 cggtaccgcc gccgatgacc acagcgacct tgccggggct gggagggcca gcgcggacta
     1561 aaccgccggg cacctgctgc acgtacttgg gaaatgcgcc ggcgaatccc gacagcgcct
     1621 cgccggagaa gtcggccgga tcgttgtaga tcttggtcat cgacaccttc tggatcagcg
     1681 gttgttcgcg attctatggg gagggtggtg ggcggggacg gccagcttta ggcgctggcg
     1741 cggtaagccg cgcggcggcc acccgctgcc ccgggtcact actcagtatt tgccatgccg
     1801 tgacgccgaa ttcgagtagt ccggtacgca tggcgacacg gcgttcacgg catagcttgg
     1861 gcgcatcaga gaaaaactga cgcggcgcca aacatgattg aaccggatcg aataccggac
     1921 cgttgaactc gacagggacg ccgctaactg aatcgcccga cccgggcgca acaacaggat
     1981 agcctgacag caaatccacc gccgcaggcg cgtggggccc tggtcaacca tgtaatcgcc
     2041 gatttgaagc cgacggttcc gtcagtttga ccgttttgca ttgggagaca actaaaaccg
     2101 caggatttgc gacgattgct ccctatgggc ggatcgacgc agagatcaca gaaactctgt
     2161 aacgaatgac tgacgaagat gcgtagcaaa tgtccatcat tcgtcacgga acacccggaa
     2221 ggtactgcca tgaagaagat cattttcacc tcactcgcag cgggcgcgtt ggcatccgcc
     2281 acactcggtc tggccggagc cgcttccgcc gcgccgtccg gcccaccgcc ggtcgatgac
     2341 accatcagtc aactgcaggc gaaaggcttc aacgtcctcg tgagccgggt cgacagcgat
     2401 ggggtggacc ggtgcacgct caacgcggtt cgcccgggcc agacgttctc gcgcaccgac
     2461 tctggtgtgc ctggagccca agacgacctt gtcaccacgg tgactgacaa gaccgtcttc
     2521 gtcgacgtcg tctgctgaga caacacacgg gcgcgggaca acgggatcgc gtcagcatcg
     2581 ttgaactatc cggcgtccga cagcacgtga gctggttcag cccagcgggg caagcggata
     2641 gatgatcttt gcctctgccg cgtccggcgg cgcgatcgcc ttgacctgcc cgccctggat
     2701 ctggaccagc atcgggctga gcgcggtgtt gttgtggaag tggtcgccct cggatgcgaa
     2761 cttgatcggt ccgtagaagg tctgcaggtc ggtgccggcg atcagctcgt tcagcttgac
     2821 ccgggcctcc tcggaaagcg acggcggctg acccagcttc tcgacggcag cctgcagcac
     2881 ctgcccggcg gcgctgcacc cggcggcggt gtaatccggc ggcgagccgt gctgctcctc
     2941 gaatgtcttc gcgtagtcgc cggccgagcc gaacaggtcg tccttgaagg acgcggtcgg
     3001 caaccacacc gagattccca ttacgccgtc ggcctggcgg ccgagcgcct gcgcgaagga
     3061 ggcatcggtg atgccgtagt gctcgatgat cgccttcggc gtgtagccgg tcgcggccat
     3121 cgctttgacg aagtcgacca gcagaacgtc gtgcccgccg accgccacaa tgtcgggtct
     3181 ctgcgccgca accacgccgg ccaccggcgc cagatcggcg ttcggcggga acagcgagta
     3241 gtgcacgagg ttgagcttcg cgtcctggac tccggtacgg aagccctcgg cggtgttgtc
     3301 ggagaacggc tcgttggcgc cgacgaccgc cgctgtggct ggcttggggt tggcgttgtc
     3361 cacgatcgcc tggatcgagc gggccgccgt ggtgtcgacg gcggggatga cgccaaaggt
     3421 gaactcgggc ttgcctttcc agacgttggg cgactccgct gagccggcga tgcacggcac
     3481 ccggtacttg gcgcagatcg ggtccatcgc gagctgcacg ccgctggtgt acgcgccgaa
     3541 tatggcgttc acgccctcct cggaaacaag ccgagatgtc gcatcggcgc ccgtggcggg
     3601 atcgctctta cagtcctgca cgatcaggtc gacttcgtac ttcttgtcgc cgatggacag
     3661 tccgcccgcc ttgttcaccg tgtcggccca cagctggtac ccgcgctggg cgatctgccc
     3721 accggtcgcg gattcgccgg acagctcgag cacggcgccg accttgaatt tgtcggtcgc
     3781 cccgccgccg ccctgcccgc cgccgcacgc ggcgatgaag ctcgggccgg caaccgcgcc
     3841 gcccatcacg aggccggccc gggtaagaaa cagtcgacgg ttgatgttgc tggacatgat
     3901 tactcctatc ggggttccct tgggccgcgg gccgcagcgg tgcatcgttc agcggccccc
     3961 taaatagagg tgcatgagct cgcggtcgtc ttccacgtcg gcgggcgtgc cttcgaagcg
     4021 gttccgtcct gccaccaaga cgtacatccg gtcggccacc cgcaacgcct cgcggatgtt
     4081 ctgctcgatc atgatcacgc cgacgccgcg cttggcgagt tcggcggcgc gcaccaggac
     4141 gtcgcctacg agcttggggg acagccccgc cgacggctcg tcgaacagta ggaaccttgg
     4201 gcgcaagatg agcgcgcggg cgatcgacag gatcatctgc tcgccgccgg agagctggcc
     4261 ggcctgcacg tcataccgct cgcgcagtcg cgggaactcc tcgagcagct cgtcgatccg
     4321 ctggtcgagt tgctttcggg agcggatcgt atagccgccg atacgcaggt tgtcgcgcac
     4381 tgacagctgt gggaacacgc cgccgccttg cggcatcatc gcgagcccgc ggccgagggt
     4441 gcgatgagac ggcaggtggg tgaggtcgtc gtcgtcgatc gtgatcttgc ccgcccatgg
     4501 gtcgatcact cccaccattg ccttcagcag ggtgctcttg ccgcagccgt tcggcccgaa
     4561 gatgcaggtg acttcgccgg tgcgagctgt catcgacacc tggtgcagga tctgcacggc
     4621 gccatatccc gcgtcgaggc cctcgacggt gacgacgccg tcgcgggagt cgctgccctg
     4681 gcgttcactc atccgccgac acccccagat acgcctccat gacgtggggg tcgtgcgaga
     4741 tctcctcgaa gctgccctgc gccacgatct ttccggcgtc catcacgatg atccggtcgc
     4801 agatgtcctg cgcgagttcc atgtcgtgct cgatgatcag gaacgtcgtg cccgcgtctc
     4861 gcagggtgcg tatggcgtcg agcatgatct ggcgcaggcg gggatgaacc ccggcggcga
     4921 cctcgtcgag cagcgcgacc ttcggcttct gcatcagcac ccgacccatc tccaacagcc
     4981 gctgctgccc gccggacaag gtgctcgcga ggtcgtcggc aacgtgggtg agctccagcc
     5041 gctccagtac ggcgtccgcg ttggccctgg cgttgtccca atcgaactgc accgcggcgg
     5101 tccaaaggtt ttcccgcaca gtcatattca cgaacagcgc tgggatctgg aaggtgcggc
     5161 tgaggccggt gcgtgcgatc ttgtaagggg cgagccgggt gacgttctgg ccgaacaaag
     5221 agatagagcc gccgtcgggc ttcagtgcgc cggtgagcag gttgaacagt gtgctcttcc
     5281 cgctgccgtt gggtccaatg atgccaacga tctccgcctg ctcaacaccg aacgtgacac
     5341 cgtcgaccgc gcggatgccg ccgaaggagc ggtgaaggtc gtcgaccgcg agcaccgtca
     5401 ctgaatggcc tcctcggtct ggttctccgt tcgccgcggt gccacgtcgt cgcggacacg
     5461 gccaagaagc tggcgtacca accgcattcg tcgtgcgcgc tggccgaggc tgaccagtcc
     5521 gtcgggcagg aacagcacga tcgccatcac cagcaggccg acgacgatga tgtatgcagt
     5581 cgtctcgccg tagctgaccc ggagctgctc ctgcaacagg aacattgcgg tcgcgccgag
     5641 cgccggaccc cacgcgtggc cgagcccgcc gacgagcacc atcaccacga gctggtcagt
     5701 gatgatggtg ttgagcacgc tggccgggtt gatgaacgtg atccagtagg cgttgatgcc
     5761 gccgagcacg gccgggatca tcgcgctcag cacgaaagcc tcgatcttga ccagcgtggt
     5821 gttgatgccc tgcgcgcgtg ctgcggattc attgtcgcgc accgccttta cctggaagcc
     5881 gaaacgggac cgctccagca gcaggtagca catggcgtat gcgatcgccg cggcgccgag
     5941 catcgtgtag aagaagaagt tgtcgtcgag gcggatcggc gtcttgagtc cgcctgagcc
     6001 accggtgaag tcgacaactg tggtgaattc gcgtactgcc tcggccagcg cccaggtcgc
     6061 gatggcgaag tacgcgccct tcagccgcag cgccggccag ccgacgagcg ccgcgaccgc
     6121 accggcaatc acggccgcga caggaagggt ggcccagaag ttccagccgc ggttgggctg
     6181 catgaggatc gcggtggtgt atgcgccgat gccgaagaag gccacatggc cgaagctgat
     6241 gtagccggcg tagccgcaga tcacgttcca ggacagcgca attccggccc acagcgcgat
     6301 gccggtggcc acccgaatgt agtacggcgt cgtcatcatc ggcaacagac agagggcggc
     6361 gacgacgagg gcaaggacgc cgaggttgag atacgggttg cccccggagg cgggtctgct
     6421 catgcgacga cctcgctggc tcggccgtcg cattccgtgc cgatgattcg ctcgcaagct
     6481 tcgctcatgc gagcccccgc cgcgagatgc cttgcgggaa caggagaagc gcgacgaaca
     6541 gcaccccgaa gaccaccagc agtgtgtagt cggcgcccac atacgtcgta accaacgact
     6601 gcaacacgcc gaggaacaga ccggcggcca tgactccgac caccgagccg agcccggcca
     6661 gcacgacgac gaagaatgcg aacagcgtgt agcgcaggcc gacctcgggg gtcatggcgt
     6721 agatggcgcc gagcatcgcg cctgcaatcg cggtgatgcc ggtgtagatg gcgtacacca
     6781 ggctgctgat gcgt
//
LOCUS       DASRJA010000669        24600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3711923,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000669 DASRJA010000000
VERSION     DASRJA010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 24600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24600
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3711923"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..677
                     /locus_tag="VFC01_29950"
     CDS             81..677
                     /locus_tag="VFC01_29950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83873.1"
                     /translation="MRGVDSFHVWSLLRACGRRNRLVRTSDRIELLIIALGVLIALAA
                     TAFAGALGTAVHDARSQAYVAEAQTRHTVIAKAIADSTTALGVRDNAVTRVHARWQVD
                     GAKHTDSFTIDGLVKTGDPLTIWVDRDGNRVDAPTPTSQAGVDAVAAAYAAWLTVVLT
                     VTGLVWWARSGLDRRRMSGWERDLRSLVYGGGSGNRKV"
     gene            729..1268
                     /locus_tag="VFC01_29955"
     CDS             729..1268
                     /locus_tag="VFC01_29955"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZN83874.1"
                     /translation="MAAPQTPSAFTDFVNVLVLVGLPVGPANRTLSRHAIDSSPDGVT
                     VNVFDDLARLPRYRDAFESERKPDAVVDLLLAAAEADAVLLVTSYRGRIPSMAHNAID
                     WLTGQWRPGALHDKPLAVVGQSAGCYSGVWSRRVEDVRGGLARRVIEPLTVPALPDVV
                     RQLADELHGGSAASAISRP"
     gene            complement(1283..1672)
                     /locus_tag="VFC01_29960"
     CDS             complement(1283..1672)
                     /locus_tag="VFC01_29960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HZN83875.1"
                     /translation="MVDEARRRSQDLQLRLADRITSFAGSMNFVWIHAALFAVWMLLL
                     ERSPWPTLTLVVSLEAIFLSTFVMIGQNRQASFQQAKADHDFEAQELELKTNTELTRQ
                     IHVLTQELHRRIIGPPSERRDSKHEPS"
     gene            complement(1950..2531)
                     /locus_tag="VFC01_29965"
     CDS             complement(1950..2531)
                     /locus_tag="VFC01_29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003894623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HZN83876.1"
                     /translation="MHRIWSSAVVSGLLAVVLGAVILAWPGPSILVTSGVFGAYLVVS
                     GVAMVVLGFSMPVASGASRFLSVISGVASVVLGILAFKHFGEGYAVLLLAIWIGVGFI
                     VRGVTVLATAIADKQFPGRGWAIFFGIVSVLAGFVVLAYPFDSIVTLALVAGIWLVVL
                     GVVEVISGIGMRSDVKKVQKVTGAGPQPHAVAQ"
     gene            complement(2887..3339)
                     /locus_tag="VFC01_29970"
     CDS             complement(2887..3339)
                     /locus_tag="VFC01_29970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83877.1"
                     /translation="MSDLFAEIGASMTMNSQDTEGATAQVSPSGATFAVGALLLTSAI
                     LTFLVGVFALITHELVASGPGYQYTFQMTGWGWVNILTGLVLAVVALVVFLNPVQARV
                     AAIAATCLAIAVSFLWLPYYPPGAIVLIALDIVVIWGVATWNTSRETA"
     gene            4198..5202
                     /locus_tag="VFC01_29975"
     CDS             4198..5202
                     /locus_tag="VFC01_29975"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN83878.1"
                     /translation="MLSASANSITDPDALARGGADAEKSAPFPSGVIPLLGPLEHRAL
                     RTRNHDDAGPNPAPTEASISHFAELVARAVVAGYRNHQNGQSVGDVSPSPFSIETLLD
                     GRVADEWTLWEVANQLRLPTSGPFVVVAAEVSSVGGEALPGIESKLRSLDVYSAWQLL
                     PDLHVGVVHIHAQQQLDKVLALVSRLAVAPVGVSARYDDLRDTPQALHFAKVMLRSRR
                     DQNSPVALFDGSILAAAAVSAPTVMVKSARAVLDGLADLTDEERDVLLETFRAWQDNE
                     ASADTAAKVLFCHPNTVRQRLRRIEKRTGRSLSRPRDVAELCLALEVHHTLIRPMHAS
                     "
     gene            5347..5949
                     /locus_tag="VFC01_29980"
     CDS             5347..5949
                     /locus_tag="VFC01_29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HZN83879.1"
                     /translation="METLLESRLWKFAMVWGALSAVLGGLILAWPGTSILVASTLFGV
                     YLLVSGFAGVAMAFTLPESAGMRVMLFISGVLSVILAVLCLRNFGNPYPVLLLSIWIG
                     VSFIVQGFSIVAVAISYKALPARGWYGVLGVLSVIAGGVVLAWPWDSIAVLTLYAGIW
                     LVVMGIVQIVQGFVMRNDSKTVREVGDVVRQHFHEDRKAS"
     gene            complement(6254..7177)
                     /locus_tag="VFC01_29985"
     CDS             complement(6254..7177)
                     /locus_tag="VFC01_29985"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN83880.1"
                     /translation="MTNLSVHLAESADRYPDASALVHDETTTSYSALANDIARFADYL
                     SYGGLQPGDRVGVMLPNGPEFAVVFYGVLHAGGVVVPLNSALHARVVEFYLTIAGARM
                     VFVPPRHAIASTVAAVTAGTQPVEIGRHGIASLTAGFAGRVEPVSRAACDAAVVLPVG
                     ETNGAHTVELTHGELADSQAEAAGLLLSLGPNDVVMGCLPMSERSGMTCGLLATMSSG
                     AALVLPAIDPAIDPGTALETIAAERVTVLEGVPAMYVALLDAAHHSDEDFSSLRVCVS
                     AGGPVPADIVRRIEDRFECVVVDPAELNAHA"
     gene            7364..8071
                     /locus_tag="VFC01_29990"
     CDS             7364..8071
                     /locus_tag="VFC01_29990"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZN83881.1"
                     /translation="MIPLSDGIATRRFPIVNVALIAANFAVFLFYELPNPDSAVSQAA
                     FYPCDVSSACHMGGLPWGLGWITAMFMHAGWDHILGNMLFLAIFGKNVEDVFGRLGYL
                     LFYFAGGFAAAMMQSGMTLLFGTASDAQIPTLGASGAIAAVLGAYFVLYPKSRVLTLV
                     LWFPVRIPSWLYLGGWFLYQFFEGNRSLVHPTGSGGSGVAFFAHVGGFVFGVIAARVL
                     VAIGPRTVNHGVAVQRG"
     gene            8077..8337
                     /locus_tag="VFC01_29995"
     CDS             8077..8337
                     /locus_tag="VFC01_29995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83882.1"
                     /translation="MTDLVAHIREIIDEHAKRDSQGDEGLACGCGAMDVADHSTHVAQ
                     AIVKRLGLRREVVDDARKAVKDKIRYASALVDEELTVLEGAE"
     gene            8397..8537
                     /locus_tag="VFC01_30000"
     CDS             8397..8537
                     /locus_tag="VFC01_30000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83883.1"
                     /translation="MHIAILTFERYNELDSLITGVLNRARETYLAEAPEHGGKPLLVF
                     EP"
     gene            complement(8596..9498)
                     /locus_tag="VFC01_30005"
     CDS             complement(8596..9498)
                     /locus_tag="VFC01_30005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HZN83884.1"
                     /translation="MTTFNQKVFVLEASGDGRLFSINPDGSGKTVIVTGCPVPDGVAV
                     DVDAGHIYWTNMGMPPANDGSIERVDVDGGNRTTIVPIGGTYTPKQLHLDAVGHKLYW
                     GDREGMRVMRCDLDGSNVETLVQTGQSDDDRRDEMNWCVGVTVDHVGGHLYWTQKGPS
                     DAGLGRILRAGIDIPAGESADRRSDVEVVFKELPEPIDLEIDHTARILYWTDRGDAPD
                     GNTVNRAPLDVIGRSEPEIVATHLMEGIGIALDPAHNRMFITDLGGNVWVAALDGSGH
                     RPIRALQGNLTGIAYAEVPDGTST"
     gene            complement(9679..10629)
                     /locus_tag="VFC01_30010"
     CDS             complement(9679..10629)
                     /locus_tag="VFC01_30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZN83885.1"
                     /translation="MTMPKSVQHIAIVGTGTIGASWATHYLAHGFDVTATDPAPGAEA
                     ALRSYVDGAWKAVTTLSLAPGASPDRLKFTSDLPAALADADFVQENAPERPDLKVKLF
                     ADIDEATPPDAIIASSSSGITMSVMQAECRRPERTVIGHPFNPPHIIPLVEVVGGTKT
                     DPETVYDAMDFYASIGKKPIHLKKELPGHVANRIQSALYREVVYLIGQGVLDVADADD
                     AVSWGPGLRWGVMGPNLLWHLGGGQGGIQHFMDTLMPRMAAGWPGHGNPDLTPELQQQ
                     IVKGVLEEADGQSLDELAAQRDQMLFDLIATRAKHEPTRG"
     gene            complement(10660..11442)
                     /locus_tag="VFC01_30015"
     CDS             complement(10660..11442)
                     /locus_tag="VFC01_30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HZN83886.1"
                     /translation="MTSTEVSTELVLTERHDGVLTITINRPARKNAISREVAVQLATA
                     LDLLDTDPTLSVGVLTGAGGTFSAGMDLKAFANGQTPILPGRGFGGLTQAVVRKPLIA
                     AVEGWALGGGFEMVLACDVIVAAEDAKFGLPEVKRGLIAGEGGVIRLPRRIPYHVALT
                     VLLTGEPMSAAHAKQYGLVSELTASGSALAGAQALAQRIVANAPLALARVKEVLRETQ
                     GLNDSEAFKRQADSASDLLTSEDAHEGALAFAEKRAPAWRGR"
     gene            complement(11439..12677)
                     /locus_tag="VFC01_30020"
     CDS             complement(11439..12677)
                     /locus_tag="VFC01_30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZN83887.1"
                     /translation="MTTQTTQSVFAGLKVVEIASFIAAPAAATMLSDFGADVIKVEPP
                     GIGDPQRLLSSVPPSPAAPGNYSWHLANRNKRGMAIDLKSKGGTEILKRLIEWADVFI
                     TNFPHGTREGLHLGYEEVSGWNPRIIYADITGFGDAGPDAKLPGFDLTAFWSRSGLLA
                     STRDAGAAPTSPPWGSGDYTTATAIYAAITTALYHRERTGRGANVGTSLLATGVWATG
                     TLVSAALAGGKPYELHDRTAPVNAMTNPYQSADGRWFMLAARQSAWPALANAIERSDL
                     LSDPRFADATAIGQNASQLADLLDVEFRTQPLAHWKKALDEARIPYGVVQSAAEAAED
                     PQLRAANIVMPIEGARDLEYTVNNPITLRGLATVPAKGAPEHGEHNTEILAELGFSAD
                     EINQLREQKAIPVAPEQEER"
     gene            complement(12690..13910)
                     /locus_tag="VFC01_30025"
     CDS             complement(12690..13910)
                     /locus_tag="VFC01_30025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN83888.1"
                     /translation="MTAKVELPTTPRPYLGDGLLPADFYSYEELLSDSEREKVARIRE
                     FFRTEAAPIVDDYWLRAEFPHELVKGFAKLGLLDWADPDSSEPAPSNLMTGITAMEMA
                     HADPSLSVFFGVHSGLAMGSILTCGSDEQKSHWLPAMSRFEKIGAFALTEPHGGSDVA
                     GGMETTARRDGDEWVINGAKRWIGNATFADIVVVWARDIETNRVLGFVVEKGTPGFTA
                     TKIENKFALRIVQNADIVLDECRVPEANRLQNANSFRDTAEILRRTRSGVAWQAVGVQ
                     FAAYELALQYAKERTQFGRPIGKFQLVQDLLVKMLGNATACAGMMVRLAQLQHEGVYR
                     DEQSALAKAYCTVQMRETVHWARELFGGNGIVLDYNIGRFVADSEALYSYEGTREMQT
                     LIVAKSVTGLSAFV"
     gene            14233..15087
                     /locus_tag="VFC01_30030"
     CDS             14233..15087
                     /locus_tag="VFC01_30030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN83889.1"
                     /translation="MAPRGNALGDFLRARRDQVRPEDVGLVPGGHRRAPGLRREELAM
                     LAGISAEYYLRLERGRAQHPSTQILEALARALQLDAKARQHLHDLAGPTEPDTGELDA
                     GVRPLAELVDQFLMPAVVANRYLDVVAANPLARALSPEFTPGQNFLCWRLLDPAAREL
                     YVDWDEATDSAVSGFREFSGLCPVGDPRLKALLAQLSAASPRFIELWGRANVGYHRWG
                     IHHMRHPQVGEIFLYRNRLNAPGPSGDHVIMYPAEPGSPSALALEELRSLSMPELGRT
                     GAAAAKSD"
     gene            15366..15575
                     /locus_tag="VFC01_30035"
     CDS             15366..15575
                     /locus_tag="VFC01_30035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83890.1"
                     /translation="MIELSEQTVIEQLVARLTSRYPSIAESTVSSVVRDVHARFDGRP
                     LRDFVPLFVERHAQSELERLSATPG"
     gene            complement(15562..15846)
                     /locus_tag="VFC01_30040"
     CDS             complement(15562..15846)
                     /locus_tag="VFC01_30040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83891.1"
                     /translation="MATPSMPTGAAGQGSDSLQAAYFRGTLADQRAVLAVHITRQHNK
                     LQSMTAAGTNVLAITQVRHQLRENEAEVRDLDRMIEAIDRRFSASWITQA"
     gene            complement(15892..16989)
                     /locus_tag="VFC01_30045"
     CDS             complement(15892..16989)
                     /locus_tag="VFC01_30045"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83892.1"
                     /translation="MPPQSTHKTLTSLRQGSMIHVYDQDFDQKVAEPFHEAYMAHTST
                     SPNYQILASLDLGRRQVALEGVELVQRQIENAMQLRDAIDNHPLLSRYMECLKTSDLI
                     PDEFRPSGIAQPLRSGLRNMMAAWDADEFVLDPSRITLSIGRTGYDGDTFKREQLMDR
                     HGVQINKTSRNTVLFMTNIGTTRSSVAFLVEVLVKIARELDEHISEMGLGERSRFEQR
                     VRRLTARAGVQPDFSGFHPVFRDQSSIDDTPEGDVRRAFYLSYNDSNCEYLSSDEIFE
                     KLDAGIDVVSATFVTPYPPGFPVLVPGQVFSREILTFIRDLDTPEIHGYQPDFGYRAY
                     TDNAIEMCRPTEATITNGHHPVPAESTLTGD"
     gene            17033..17302
                     /locus_tag="VFC01_30050"
     CDS             17033..17302
                     /locus_tag="VFC01_30050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83893.1"
                     /translation="MEHFLVDRLIIGIGGQLGFELTLVFVVAQLVSQIASRRHRGPIA
                     VYRVEPGEREPCFVPEEHQIRLDGQAFLHDALDVVDDAVEGAVGQ"
     gene            complement(17192..17704)
                     /locus_tag="VFC01_30055"
                     /pseudo
     CDS             complement(17192..17704)
                     /locus_tag="VFC01_30055"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            complement(18796..19581)
                     /locus_tag="VFC01_30060"
     CDS             complement(18796..19581)
                     /locus_tag="VFC01_30060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN83894.1"
                     /translation="MTRYEHLIYEQRGPVTIITINRPERMNAIGPQTHRELVDAWDRF
                     RADDHAFVGVLTGAGDDAFCAGGDLKAAFAGELEVDASPADGVLGPSRWTNIYKPTIA
                     AVNGVAYAGGLEWACWTDMAIADEHATFGVTCRRWNIGLADGGTQRLTRILGYRRAID
                     LIVTGRVIDAREAERIGLVNEVVPKGSCLQRAVELAEAIATLPQPALRTDLEAARRGL
                     GRPLDDGLRIEAECFNRSIVEPETVEGLRRFNERDHPDLARSH"
     gene            complement(19578..20744)
                     /locus_tag="VFC01_30065"
     CDS             complement(19578..20744)
                     /locus_tag="VFC01_30065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN83895.1"
                     /translation="MSTPTKASVADDAEIVRRIFAHIDAGTTDVGEAWREPVQNYLDP
                     IRFTHELEMLRSFPSVYLPSAAIPNAGDHAERVTFGVPLFGVRGKDGRARVFRNSCRH
                     RGMALVEGPGCSHALVCRYHGWTYRLDGTLSHVPHPEAFPDLDMPTRGLVEVDSREVD
                     GLIVIGPLSPESGRDTDTAMAALTDGSPWRDKLLPAERLFAVRPALRDMNWKVLVEQF
                     LEGYHIRTTHKDTFFPIQYDDLNVVEAFGPNSRITFPYKNIERLRDRPESTWNVGHRV
                     TYVYHLFPNVMVATFPNQVLVITIDPIDVDHTTVTIYAMVTPHVAERVSANPEDAAGA
                     RNLLNEGGVEDNEMSEGVQRGLHAGANTFVEFGTHESAIGRFHATLDARLAHSA"
     gene            20805..21605
                     /locus_tag="VFC01_30070"
     CDS             20805..21605
                     /locus_tag="VFC01_30070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZN83896.1"
                     /translation="MPIVDERRAHVADLIGDQSLSARSVLATALLGADQPHLTVGELV
                     AMASLFGISDGAARTCLWRMVSNGELTSDDGMYALAGGLLERRERVDEAARFAATRQW
                     DGTWELAVVSLERRSAADRLALRKAASALHLAELREGVWIRPDNLDPDRLPTLRAVLD
                     RQCVHFHGAASDIPGETVRSLFNLDDWTADARRFSRAVHDEVDAEAVAADDFVYWFAL
                     SVAVVRHLQLDPLLPVEFLPEQWPGHDLRSAYRRFDEVFKQRLNTAFR"
     gene            complement(21621..22241)
                     /locus_tag="VFC01_30075"
     CDS             complement(21621..22241)
                     /locus_tag="VFC01_30075"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN83897.1"
                     /translation="MHVSRAVSVGGRRTQAERRARTRAALLEAAARGFSHYGYGNVVL
                     ERVANEAGYTRGALYHLFQGKEDLALAVVEWVAESWEREVWQPAQRKGEAVDVLVALV
                     RGHIVFCRRDIARVMMALRVEFAGRDHPVGQKVGEIGKGLALRFGALITAGRRDGSIP
                     AGPPARILATAAIGAVEGLAIEVAGVRGDELIAERMMRGILGLEAR"
     gene            22279..22755
                     /locus_tag="VFC01_30080"
     CDS             22279..22755
                     /locus_tag="VFC01_30080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83898.1"
                     /translation="MKLPNAEDGTTQRARRCSVEEPPEGHAHYDYADAFEVTLADGDP
                     RSAEQVVRDGLARAPSALRAVILIAHRRVLRLNVGPDFWPIVASEPDMVRLEAAGSLI
                     AGALVARRIGGRTARLTTFVTYQGPFARIVWPIVAPLHRRVAPYLMERAARPAESV"
     gene            complement(22733..>24600)
                     /locus_tag="VFC01_30085"
     CDS             complement(22733..>24600)
                     /locus_tag="VFC01_30085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HZN83899.1"
                     /translation="GYRELFDASISDPAAFWADAAKAVTWTREPQQILDDSNPPFYRW
                     FPDGELNTCANALDRHVDGGRAEQPALIYDSPVTGSQRTYTYRELLDQTARFAAALRG
                     LGVDKGDRVVIYMPMVPEAAIAMLACARLGAVHSVVFGGFAANELAARIDDAQPAVIV
                     SASCGVEPTRTVDYKPMLDAALEVAEHAPPACVILQREQRPCEPLAGRDHDWHELMAD
                     AQPVDAVPVAATDPLYVLYTSGTTGKPKGIVRDNGGHAVALLWSMRNIYDTHPGEVYW
                     AASDVGWVVGHSYIVYGPLMLGATTVLYEGKPVGTPDAGAFWRVASEHGVKAVFTAPT
                     AIRAIKKEDPEGLHLSKYDLSALKYLFQAGERLDPGTYGWASDILGIPVVDHWWQTET
                     GWAIAANPMGIEPLPIKPGSATVPMPGYDVHILRPDGSECDANEEGAICVRLPLPPGT
                     LPTLWGADDRYVSSYLSAFDGFYLTGDGGYFDEDGYLFVMGRTDDVINVAGHRMSTGS
                     IEAVLAGHPAVAECAVIGVADEIKGQVPRGFVVLKAGATAEGLADELIDAVRENIGAV
                     AYFKLVDVVPALPKTRSGKILRKTMRGIADGRDEPVPSTIEDAAVLDALKPILRA"
BASE COUNT         4287 a         7841 c         7815 g         4657 t
ORIGIN      
        1 accgaccacg tcggcgtcac caataacgac gccgcgtcca gctcagcggt cccgagccgg
       61 tgacgagtag gtgaatggtc atgcgaggcg tcgactcctt ccatgtgtgg tcgctactgc
      121 gtgcgtgcgg ccgccgcaat agactggtac gcacgagcga ccgcatcgaa ctattgatca
      181 tcgcgctcgg tgtcctgatc gctctggccg ccaccgcatt tgccggcgca ctggggacgg
      241 ccgtgcatga tgctcgcagc caggcgtacg tcgcggaagc acagacacgg cacaccgtga
      301 tcgcgaaggc catcgcggac agcacaaccg cgttgggcgt ccgcgacaac gcggtgacac
      361 gcgtacacgc ccgttggcaa gtcgacggcg ccaaacatac tgacagtttc actatcgatg
      421 gtttagtgaa aacaggtgat ccgttgacga tttgggtcga ccgtgatggc aaccgcgtcg
      481 atgcacccac cccgacgtcg caggccggcg tggacgcggt cgctgcagcc tacgcggcct
      541 ggctaacggt ggtcctcacc gtaaccggat tggtctggtg ggcgcgctcg ggcctcgacc
      601 gtcggcgcat gtcgggctgg gaacgcgatc tgcggagcct cgtttacggc ggcggcagcg
      661 gaaaccgcaa agtttgatag ctctgctcca gtacgttcca aatcgaatcc ggaaaaggca
      721 gcaaaacgat ggcagctcca caaacaccga gtgcattcac ggacttcgtc aacgtgttgg
      781 ttttggtcgg tctccccgtg ggtccggcca accgtacact tagccgacat gcgatcgaca
      841 gctcgcccga cggtgtaacg gtgaatgttt ttgacgatct agcgcgcctg ccccgctacc
      901 gcgatgcctt tgaatccgag cggaagccgg acgcggtcgt cgacttgctt ttggccgctg
      961 ctgaggcaga tgcggtgctc ctcgttacga gttaccgcgg gcgaattcca tcgatggcgc
     1021 ataacgcaat cgactggctg acaggtcagt ggcggcccgg cgcactgcat gacaagccgc
     1081 tcgccgtcgt cggccaatcc gccggatgct acagcggggt ctggtcgcgc cgagtcgagg
     1141 acgtgcgcgg aggtttggcc cgccgtgtga tcgaaccact gacagtgccg gccctgcccg
     1201 acgtagtcag gcagctcgca gacgaactgc acggaggcag cgctgctagt gcgatttctc
     1261 ggccctgacc gtttggtccg atctacgatg gctcatgctt ggagtcgcga cgctcggacg
     1321 gcggcccaat gatccgtcgg tgcaattcct gtgtcagtac gtggatttgg cgagtgagct
     1381 cggtgttggt cttcagctca agttcctgag cctcgaagtc atggtcggcc ttggcctgtt
     1441 ggaaagacgc ctgccggttc tggccgatca tcacaaaagt cgacaagaag atcgcctcca
     1501 gcgacacgac cagggtcaag gtcggccacg ggcttcgctc caggagcagc atccagacgg
     1561 caaacagcgc cgcgtggatc cacacgaaat tcatcgaacc ggcaaaggag gtgatccgat
     1621 cggcaaggcg tagctgaagg tcttggcttc gtctgcgcgc ctcgtcgacc accgcgggat
     1681 ggtggacctg tccgctgcgc aatagccggc gtggaaccag ttgggggcgg cgccgacggt
     1741 cttcgtcctg tgtagtcatc gtaattcctt cctgcccaag tcaactggct cgctcagcta
     1801 ccttgtacgt cggtgcgccg gtgagcgaag gcgcgtctga ggattcaccg tcatgatcgc
     1861 gggccgggtc gccctggcag atgtgccagt gacgatccgg cccgcgatgg cccagctatc
     1921 cgtgcattga acacggcgtc gctaacgggt cattgtgcaa cggcgtgcgg ctgcgggcca
     1981 gcgcccgtga ccttctggac tttcttgacg tcgctgcgca tgccgatgcc ggagatgacc
     2041 tcgaccacgc caagcacgac cagccaaatg ccggcgacca acgccaacgt cacgatcgag
     2101 tcgaatgggt atgccaacac cacaaaaccg gcaagaacgc tcacgatgcc gaagaagatc
     2161 gcccacccgc ggccggggaa ctgcttgtcg gcaatcgcgg tggccagcac cgtcacgcct
     2221 cggacaatga atccgacacc gatccagatc gccaggagca gcaccgcata tccttcgccg
     2281 aagtgcttga acgccaagat cccgaggacg accgacgcga caccgctgat gacgctcaag
     2341 aatctactag cccccgaggc gacaggcata ctgaacccca ataccaccat cgcaacgccc
     2401 gacaccacaa ggtaagcgcc gaacactccc gacgtcacca ggatcgacgg gcccggccat
     2461 gccaggatca ccgcaccgag gaccaccgcc agtaggccgg acacgaccgc tgacgaccag
     2521 attcgatgca tgaaatttgc agcgggactg tctgtttgac tcactttctt ctccttttct
     2581 gtgtactcac gcacgcgatg ttttggttgt ctgagccatc actgagtgag aacgttgttt
     2641 gttgacgatt actacgatcg gccacgcggc aagcccagca ccatcgcccg cggattgatg
     2701 tttaaggctg tatggatgta gtaccaagcg ggtcggtgca tgaacgggta tcaagcacgc
     2761 cacagtggtt gtcggattct gttgtgtctc tgtagaaaag cagagccccg atgacaacca
     2821 tcgcagtgac ttgtcatcgg ggctcgccta accgtttttc cggtgttgtg gctgaaggtc
     2881 gatatctcat gccgtttcgc gtgacgtgtt ccacgtcgcg acaccccaaa taaccacgat
     2941 gtccagggca atcaggacaa ttgcgccggg tgggtaatac ggcagccaca ggaaactgac
     3001 tgcaatggcc aagcacgtcg ctgcgattgc cgcaacccgt gcctgcaccg gattgagaaa
     3061 gacgacaagt gcgactacgg cgagcaccag tcccgtcaga atgttcaccc atccccaccc
     3121 cgtcatttga aacgtgtact ggtagccggg tcccgaagcc accaattcgt gggtgataag
     3181 cgcgaagaca ccgacaagaa aggtcaatat cgcgcttgtc aagagcagcg cgcccacggc
     3241 gaacgttgca ccgctcggtg acacttgcgc ggtcgcaccc tcggtgtctt gtgaattcat
     3301 ggtcatgctg gctccgatct ctgcgaacaa atcactcatg ctgcaagcgc tttgagcggc
     3361 cttgcggcgg acgattcaat cgtcggccgg gtgcgcgtgg gcgtcgatgt cactgcggac
     3421 agaatcgcgc gccgccaaat gtcatcaccg acaccacagg tcaggtctcc gagagaagca
     3481 cgtcgcgggc acctcggagt gcccacgtag gtctgagcgc cgaattcatc ggcgacttcg
     3541 gtcatggaca tcccgccgac acagagccga tcgagctcgt tgacggtcgt ggtcaacggc
     3601 cagatcgccg gatcgatgag tggcgagggg gcgccccgcg acgagggcaa ggtctcgagg
     3661 acggtcatcg aacgcccatg cctgcgccgc cgcacgtcca tcctgcgttc ctgctggacc
     3721 gcaacagttg tcttccagca acagccttca tggtgctccc ctcacgactc cttcggatgg
     3781 ccatctcacg agggttcaag gcgtcgaagc ggtccgccga tgtcgccacg gacagcacag
     3841 cgcaatcatt ggtgtgcccg atcacatcgc cagttacctc gagtcgtaga cgatagagat
     3901 gactggaaca cgtcacgcga aacggcatga atcgcagccc gccgatcgcg cacggcgcgg
     3961 ccagccgcag gtcgcggcct gcgacagctc ataaggccgc actaatagcc agcccgtcaa
     4021 atcctggccg tccttagcgc tacatccacg tggctttaaa catcaatcct cgaccgatgg
     4081 tgccgcgctg cacgtgcatg gaccgtatta gttgtccacc aatcacgttc tccgtcgagc
     4141 gtcggcccgg gccacggcac ccaccgcgcc agtgagtaca cgaggaaaga gcaaacaatg
     4201 ctgagtgcat cggcgaacag cattaccgac cccgacgcgc tagcacgagg cggggccgac
     4261 gcggagaaga gtgctccttt tccatctggc gtgatcccgc tgcttggacc gctcgaacac
     4321 cgcgctctgc ggactcggaa tcacgacgat gccggtccaa acccagcccc caccgaagcc
     4381 agcatcagtc acttcgccga acttgtcgca agggcggtgg tggccggata ccgcaaccat
     4441 cagaacgggc agtcggtcgg tgacgtgtca ccgagtccct tctcgatcga gaccttgctg
     4501 gacgggcgag tggctgacga gtggacctta tgggaagtgg ccaaccaact tcgtctaccg
     4561 accagcggcc cgttcgtggt cgttgccgcc gaggtttcgt cggtgggcgg cgaagcgctg
     4621 cccggcatcg aatcgaaact gcgcagcctt gacgtgtact cggcgtggca gctgctgccc
     4681 gatctgcacg taggcgtcgt gcacattcac gctcagcagc aactggacaa agtcttggcg
     4741 ttggtgtctc gattggccgt agcgcccgtc ggcgtgagcg cccgctacga cgatttgcgt
     4801 gatacgccac aggcgttgca tttcgcgaag gtgatgctgc gcagccgacg cgaccagaac
     4861 tcaccggtgg cgctgttcga cggctccatc ttggccgctg cagcggtaag cgccccaacg
     4921 gtgatggtca agtcggctcg cgccgttctc gatggtcttg ccgatctgac agacgaggag
     4981 cgggacgttc tcctcgagac attccgggcg tggcaggaca acgaagcatc ggcggatacc
     5041 gcggcgaagg tgcttttctg ccatcccaac accgtgcgcc aacggttacg gcgtatcgag
     5101 aagcgcaccg gccgttcgct ttcgcggcca agagatgtag ctgagctgtg tttggcgttg
     5161 gaagtgcatc acacgctcat ccggccgatg cacgccagtt gaccgcgatg ttcgccgtga
     5221 caccggagac cgtcatgtgc tgtccgcaac aacagcgaca ccacaagagc gctgattgac
     5281 catttaatgg tccggcgtaa acccgcgccg ttactgacat tccatattcg agacgtgagg
     5341 agcgtcatgg aaactttact tgaaagcaga ctctggaaat tcgcgatggt atggggagcg
     5401 ctatctgctg tcctgggcgg cctgatcctc gcatggccgg gcacatcaat cctcgtggcg
     5461 tcgacgctgt tcggcgtcta cctgctggtg tcgggcttcg ccggagtggc aatggcgttc
     5521 acattgcccg agtccgccgg tatgcgggta atgctgttca tcagcggtgt gctgtcagtg
     5581 atcctggcgg tcttgtgctt gcgcaacttc ggcaacccat atccggtctt gctgttgtcg
     5641 atttggatcg gtgtcagctt tattgtccaa ggcttctcga tcgtggccgt cgcgatcagc
     5701 tacaaggcgc tgccggcccg cggatggtac ggggtacttg gtgtcttgtc ggtgatcgcc
     5761 ggcggtgtgg tgctcgcgtg gccgtgggac tcgatcgctg tgcttactct ttatgccggc
     5821 atctggctgg tcgtgatggg gatcgtccag atcgttcagg gattcgtgat gcgcaacgat
     5881 tccaagaccg tccgggaggt tggagacgtg gtgcgccaac actttcacga agacaggaag
     5941 gcaagctgat tcggtgacag tcaaccgtca ctgagaagac acaattgaat tggcttgggc
     6001 ggtccgcagc gctgcggatc gcccaagccg ccgttgggat taactgtcgg tgttccaccc
     6061 gtcgcgtatt cccgtaacag ttccccgaat accgccggcg acgcccatgg cccacgccat
     6121 ccccggccga cccgaaactg actgactgcc tttgtgccag ccgtcgcgga tcccatcggg
     6181 ggctccctga gcaccactga ctgcaccctc gacagcggcc ccaacgacct tgccagctaa
     6241 ccgagacatc gcctcacgca tgcgcattca actcggccgg gtccacgacg acgcattcga
     6301 acctgtcttc aatacggcgc acgatgtccg cgggtacagg cccgcccgcc gatacacaga
     6361 cccgcagtga cgagaaatcc tcgtcggagt ggtgggccgc gtccagcaat gccacataca
     6421 tcgccggcac gccttcgagc accgtgacgc gttcggcagc aatcgtttcc agtgcagtcc
     6481 ctgggtcaat cgcagggtcg atcgcgggca acacgagcgc cgcgccggag gacatggtgg
     6541 ccagcaggcc gcatgtcata cccgaccgct cagacatcgg tagacatccc atgacaacgt
     6601 cgtttgggcc gagggacagt agcagtcctg cggcctcggc ctggctgtcg gcaagctctc
     6661 cgtgggtcag ctcaaccgta tgagcaccgt tcgtctcgcc gacaggtaag acgacggctg
     6721 cgtcgcacgc ggcacgactc actggttcga cacgaccggc gaatccggct gtcaatgacg
     6781 cgatcccgtg tcttccgatc tcgacgggtt gcgtaccggc agtgaccgcg gcgacggtac
     6841 tggcaatcgc gtgtcgcggc gggacgaaca ccattctcgc gccggcgatc gtgagataga
     6901 actccaccac acgcgcgtgc agcgccgagt tcagcggtac tactacgccg ccggcatgca
     6961 ggacgccgta gaacaccacc gcgaactcgg gcccgttggg cagcatgacg cccacccggt
     7021 cacccggctg caggccgcca tagctgaggt agtcagcgaa ccgcgcgata tcgttggcta
     7081 gcgccgagta gctcgtcgtc gtttcgtcgt gcaccagggc actggcatcg gggtatctgt
     7141 ccgcggactc ggctagatgc acactcagat tggtcactgc gactcccacg tcgatgactc
     7201 gggttgcccc atcatcggac caccagactg cgatgtcgat gggcgtggcg acaacgcgga
     7261 cgccctaccg gtgttccggc gaacaccgtc atagcggcat cgccgccata ccctcaatgt
     7321 ccatggtgtc gaacgatatc gagccgcagg tactggaggc cgcatgatcc ccttgtccga
     7381 tggcattgcg acccggcgct ttccaatcgt caacgttgcg ctgatcgctg ccaacttcgc
     7441 ggtcttcctg ttctacgaat tgccgaaccc cgattcggcg gtcagccagg cggcgttcta
     7501 tccctgcgat gtcagcagcg cctgtcacat gggcggccta ccttggggac tcggctggat
     7561 cacggcgatg ttcatgcatg ccggctggga ccacatcctc ggaaacatgt tattcctggc
     7621 catctttggc aaaaacgtcg aagatgtgtt cgggcgcctc ggctacttgt tgttctactt
     7681 tgccggagga ttcgcggcgg cgatgatgca gtctggaatg actctgctct tcggcacagc
     7741 gtccgacgca cagataccga ccctcggcgc cagcggcgca atcgcggccg ttctcggcgc
     7801 ctatttcgtg ctctatccca agtcacgtgt cctcacgctc gtcttgtggt tcccagtcag
     7861 aatcccgtcg tggctttacc tcggcggttg gtttctctac caatttttcg agggcaatag
     7921 atcactggtt catcccaccg gcagcggcgg cagcggtgtc gcgttcttcg cccacgtcgg
     7981 cggtttcgtc ttcggtgtga tcgccgcaag ggttctggtc gcgatcggcc cacgcacggt
     8041 caaccacggc gtcgcggtcc agcgggggta ggagatatga cggatctagt cgcgcatatc
     8101 cgcgaaatca tcgacgagca cgccaagcgt gatagccagg gcgatgaggg gctcgcttgt
     8161 ggctgtggag cgatggacgt agcagaccac tccacccacg tcgcgcaagc gattgtcaag
     8221 cggcttggat tgaggcggga agttgtcgac gacgccagga aggctgtcaa agacaagatc
     8281 cgctacgcca gcgcattggt cgacgaagag ctgacagtgc tcgaaggtgc tgagtgatcc
     8341 cttgtatgca tgacaattct ttgatcgtac tgtgtcaggg ctcatacgct tgagtcatgc
     8401 atatcgccat cctcacgttc gagcgctaca acgaactcga ctcgctgatc acaggcgttc
     8461 tcaacagggc tcgcgagaca tatttggccg aagcacccga acacgggggt aaaccgctgc
     8521 tggtgttcga accatagtga ggcgtcgccg tgagcccacc gaagccggaa ccgatcacga
     8581 cgacgcgatg cgtgctcacg ttgaggtccc atcgggtacc tcggcgtacg caatgcccgt
     8641 gaggttgcct tgcaatgcgc ggatgggtcg gtgtccggat ccgtccagag cggccaccca
     8701 gacgttgccg cccaggtcgg tgatgaacat ccggttgtgc gctgggtcca gcgcaatgcc
     8761 gataccttcc atcagatggg tcgccacgat ctccggttcg ctgcggccga tgacgtccag
     8821 cggcgcacgg ttgacggtgt tgccgtccgg cgcgtcgcca cggtcggtcc agtacaggat
     8881 ccttgcggtg tggtcgatct cgagatcgat tggctcgggc agctctttga acacgacctc
     8941 gacatcgctg cgacggtccg cggattcgcc tgcgggaata tcgattccgg cgcgcaggat
     9001 ccggcctagt ccggcgtcgc tcggaccctt ctgggtccag tacagatgcc cgccgacgtg
     9061 gtcgacggtg acgcccacac accagttcat ctcgtcgcgg cggtcgtcgt cactctgccc
     9121 ggtctggacc aaggtctcca cgttcgaccc gtccaggtcg cagcgcatca cccgcatgcc
     9181 ttcccggtcg ccccagtaca acttgtggcc cacggcgtcg agatgaagct gcttgggggt
     9241 gtacgtgccc ccgatgggaa cgatcgtggt gcggttgccg ccgtcgacgt ccacacgttc
     9301 gatcgagccg tcgttggccg gcggcatccc catatttgtc cagtagatat ggccggcatc
     9361 cacatcgact gcgacaccgt cagggacggg gcagcccgtg acgatgaccg tcttgccgga
     9421 gccgtcggga ttgatggaaa acaggcgtcc gtcgccgctg gcttcgagca cgaacacctt
     9481 ttggttgaag gtagtcatca ttaggtcctt ttcgttgtaa acagcttggt gtcgaggcat
     9541 tcggtgtacg ggacgagctg agcgtgatgt cggcgatcag cgccgcttgg tggcgcgaca
     9601 tcagcacgta acctgcgatg gctaatgttg ttggcatcat tgacgacacg ccgtacatca
     9661 cgctcagcat ggttgctcct aaccgcgggt tggttcgtgt ttggcgcgcg tggcgatcag
     9721 atcgaacagc atctggtctc gttgggcggc gagttcgtcg agcgactggc cgtccgcttc
     9781 ctcgaggacg cccttgacga tctgctgctg aagctccggg gtgagatccg ggtttccatg
     9841 tccgggccac ccggcggcca tgcggggcat cagcgtgtcc atgaaatgtt ggattccgcc
     9901 ctgaccgccg ccgagatgcc acagcagatt gggccccatc actccccagc gcaggccggg
     9961 accccacgac accgcatcat cggcgtcggc gacgtctagc acgccctggc cgatcagata
    10021 aaccacttcc cggtagagcg ccgactggat gcggttggcc acgtggccgg gcagctcctt
    10081 cttcaggtgg atgggcttct ttccgatcga ggcgtagaag tccatcgcgt cataaaccgt
    10141 ctccgggtcg gttttcgtcc cgccgacgac ctccactagg gggatgatgt gtggtgggtt
    10201 gaaggggtgg ccgatgacgg tacgttccgg gcgccggcac tcggcctgca tgacgctcat
    10261 cgtgatgccc gacgaactcg acgcgatgat cgcgtcgggc ggcgtcgcct cgtcgatgtc
    10321 ggcgaacagt ttgactttca ggtcagggcg ctcgggcgcg ttctcctgaa cgaagtcagc
    10381 gtcggcgagg gccgccggca ggtccgacgt gaacttcagg cggtccgggg aagcccccgg
    10441 cgcgagactc aatgtcgtca cggctttcca ggcgccgtcg acgtaggacc gcagcgcggc
    10501 ctcggcgccc ggtgccggat cggtggccgt gacatcgaat ccgtgcgcga gatagtgtgt
    10561 cgcccaactc gcgccgatag ttcccgtacc cacaattgcg atgtgctgca ccgactttgg
    10621 catagtcatg attgcttctc cttcaattca ggttggttgt tagcgcccgc gccaggcggg
    10681 tgcacgcttc tcggcgaatg ccagcgcgcc ttcgtgtgcg tcctcgctgg ttagcaggtc
    10741 cgatgcggaa tcggcttgcc gcttgaacgc ctcagagtcg ttgaggccct gagtttctcg
    10801 caggacttcc ttcactcgtg cgagcgcaag cggtgcgttg gcgacgatgc gctgagcaag
    10861 cgcctgcgcc ccggccaagg cggaccccga tgcggtcagc tcgctgacga gtccgtactg
    10921 cttggcgtgc gcggccgaca tcggctcgcc ggtgagcaac accgtgagcg cgacgtggta
    10981 agggattcgc cgcggcagac ggatcacacc gccctctccg gcgatgagcc cccgtttcac
    11041 ttccggcaga ccaaatttgg cgtcctcggc cgcgacgatc acatcgcatg ccagcaccat
    11101 ctcgaagccg ccgccgagcg cccatccctc gacggccgcg atcagcggct tgcgcaccac
    11161 cgcttgggtg aggccgccga atccgcgacc ggggaggatc ggagtctgtc cgttggcgaa
    11221 tgccttgaga tccatgcctg cgctgaacgt cccgccggcg ccggtgagca cgcccaccga
    11281 cagcgttggg tctgtgtcga ggagatcgag ggcggtcgcc agttgcacag cgacctcgcg
    11341 gctgatggcg ttcttccgcg ccggcctgtt gatggtgatc gtcagaacac cgtcgtgacg
    11401 ttcggtgagc accaactccg tcgatacttc ggtcgaagtc atctctcctc ctgttctggc
    11461 gcaacgggaa tggccttctg ctcccgtagc tgattgattt catccgcgct gaatcccagt
    11521 tctgcgagga tttcggtgtt gtgctcgccg tgctcgggag cgcccttggc tggcaccgtg
    11581 gccagcccgc gaagagtgat cgggttgttg accgtgtact cgaggtccct ggcgccttcg
    11641 atcggcatga cgatgttggc cgcgcgcagc tgaggatcct cggccgcctc tgcggccgac
    11701 tggacgacgc cgtaggggat gcgggcctcg tcgagcgctt tcttccagtg cgccaacggt
    11761 tgcgtcctga attccacgtc gagcaggtcg gccaattgtg aggcgttctg gccgatggct
    11821 gtcgcgtcgg cgaagcgtgg atcggacaac aggtcggacc gctcgatcgc gttggccaat
    11881 gccggccatg ccgactgcct ggcggcaagc atgaaccagc ggccgtccgc gctctggtac
    11941 ggattggtca tcgcgttcac cggtgcggta cggtcatgca gctcgtaggg cttgccgccg
    12001 gcaagtgccg ccgacaccag ggtgcccgtc gcccatacgc ccgtcgccag cagcgacgtg
    12061 ccgacgttgg cgccccggcc cgtgcgttcg cggtggtaca gcgcggtcgt gatcgcggcg
    12121 taaatcgcgg tggcggtggt gtaatcgccg ctgccccatg gcggcgacgt cggtgcggca
    12181 ccggcgtcgc gggtcgaggc gagcagcccg ctgcgtgacc agaacgcggt caggtcgaaa
    12241 cctggaagct tggcatccgg tccggcatct ccgaacccgg tgatgtcggc gtagatgatc
    12301 cgtggattcc agcccgacac ttcttcgtag cccagatgca ggccctcgcg ggtgccgtgc
    12361 gggaagttgg tgataaacac atcggcccat tcgatgaggc gcttgaggat ctccgtcccg
    12421 cccttcgact tcaggtcgat cgccatcccg cgcttgttgc gattggccaa gtgccagctg
    12481 taatttcccg gcgctgccgg actcggcggc accgagctca gcaaccgctg ggggtcaccg
    12541 attcctggtg gttcgacctt gatgacgtcg gcgccgaagt ccgacagcat cgtggcagcg
    12601 gccggggcgg cgatgaagct cgctatctca acgactttca aaccggcgaa cacggattgc
    12661 gttgtctgtg tggtcatttt catcatcctt tagacgaacg cgctcaggcc ggtgaccgac
    12721 ttggcaacga tcagcgtttg catctcacgg gtgccctcat aggagtagag ggcctcggag
    12781 tcggccacga agcgaccgat gttgtagtcg agcacaatgc cgttgccgcc gaacaactcc
    12841 cgcgcccagt gcacggtctc gcgcatctgg accgtgcagt aggccttcgc cagcgcggac
    12901 tgctcgtcgc ggtaaacacc ctcgtgctgc agctgggcca ggcgcaccat catgccggcg
    12961 catgcggtgg cgttgccgag catcttgacc agtaggtcct gaaccagctg gaacttgccg
    13021 atgggtcggc cgaactgggt gcgttccttg gcgtactgca gagcgagttc gtatgcagcg
    13081 aactggacgc ctaccgcctg ccacgccaca ccgctacgtg tccggcgcag gatctcggcg
    13141 gtgtcccgga acgagttcgc gttctgcagc cggtttgcct caggaacccg gcactcgtcg
    13201 agcacgatgt cggcgttctg cacgatccgc agcgcgaact tgttctcgat cttggtcgcg
    13261 gtgaaccctg gcgtgccttt ctccaccacg aagcccagca cccggttggt ctcgatatcg
    13321 cgagcccaca ccacgacgat gtcggcgaat gtggcgttcc cgatccagcg tttggcgccg
    13381 ttgatgaccc actcgtcacc gtcgcgccgg gcggtggtct ccatgccgcc cgccacgtcg
    13441 gagccgccgt gcggttcggt caaggcgaat gcgccgatct tctcgaagcg gctcatcgca
    13501 ggcagccagt gactcttctg ctcgtcggat ccacaggtga ggatggaccc catcgccagc
    13561 cctgaatgca ccccgaagaa caccgagagc gacggatcgg cgtgcgccat ctccatcgcg
    13621 gtgatgcccg tcatcaggtt gctcggcgcg ggctccgacg aatcgggatc ggcccaatcc
    13681 agcaggccga gcttcgcgaa gcccttgacg agttcatgcg ggaactcggc ccgcaaccag
    13741 tagtcgtcga cgatcggcgc ggcctccgta cggaagaact cgcggatacg ggcgaccttc
    13801 tcccgctccg aatccgacag cagctcctcg taggaataga aatcggccgg cagcagcccg
    13861 tcccctagat agggccgcgg cgtcgtcggc aactccactt tggcggtcat ggcgtcctct
    13921 cttgtctgtc tcgtttcagc catgcgttca ccatcgggcc ggtgcagatg aggcggggat
    13981 gccgtgttga tcctgtgtac tggcaggaca caggttcccg tgaagtggcc gaaagggcgt
    14041 tgccctgcgg tttccgccct tagagcgcga cccaggaagg actcgattac cgctagccga
    14101 ggtattgaaa tccctgcagg tttctattgc agcgaagatg aaccggaggt tcgcggctgt
    14161 cgattcacaa cctcgcgcag ctagtgcccg acgtcgcggt ggacacctcg cttcccggga
    14221 taggctcatg ccgtggcgcc gagaggcaac gccttgggtg atttcctgcg tgcgcggcgg
    14281 gaccaggttc gtcccgagga tgtcgggctg gttcccggcg gacatcggcg cgcgccgggt
    14341 ttgcgtcgcg aggaactggc tatgctggcc ggcatcagcg cggagtacta cctgcgactc
    14401 gagcggggcc gcgcccaaca tccatccacc cagattcttg aggctctagc gcgggcgctg
    14461 cagctggacg ccaaagccag gcagcatctc cacgacctgg cagggccgac agagccggac
    14521 acgggagagc tggatgcagg ggtccggccg ttagccgaac tagtcgatca gtttttgatg
    14581 ccggccgtcg ttgccaacag atatctagat gtcgtggcgg caaatccgct cgcgcgtgcg
    14641 ctgtcaccag agttcacacc aggacagaac tttctgtgtt ggcgcctatt agaccccgct
    14701 gcccgggagc tctacgtcga ttgggacgag gcgaccgatt ccgcggtgag tggtttccgc
    14761 gaattcagcg gtctgtgtcc cgtgggagat cctcgcctca aggcgttgct tgctcaattg
    14821 tccgctgcca gtccacgttt catcgaactg tgggggcgcg cgaatgtcgg ttaccaccgg
    14881 tggggaatcc accacatgcg tcatccccaa gtcggggaaa tatttctgta ccgcaaccgg
    14941 ctcaacgcac caggtcccag tggcgaccac gtaatcatgt accccgccga gcctggtagc
    15001 ccctcggctc tggcactaga ggaattgcga tccctgtcaa tgcccgagct tggccgtacc
    15061 ggcgctgcgg cggccaagtc cgattgacag gtatttgccg ggactgcctt acgatcgggc
    15121 gctagggcgg tgcagctgca agcaatccgc ttaattcttc ggtgggcggg acaacaggca
    15181 cgtcccattc acgcttgcgt cacgatgacg ggaatgcttg gcgccgttat gctttcgttg
    15241 gtagcccgcg cggcgaagct gatgctgtgc aagttcacca cctccggcca actagtggcg
    15301 atgcctgcca cttggcaccg ccggaataag ttgacggttt ggtgaacagg cgtcaacctt
    15361 cgggggtgat cgagttgagt gaacagacgg tcatcgagca gctagtcgcg cgtctgacca
    15421 gtcgatatcc gagcattgcg gagtcgacgg tgtcgtccgt cgttcgtgac gtgcacgcca
    15481 ggttcgacgg cagaccgctg cgcgacttcg tcccgctgtt cgtcgagcgc cacgcgcaaa
    15541 gcgagctcga gcggctatcc gctacgcctg ggtgatccac gaggccgaga atcgccggtc
    15601 tatcgcctcg atcatgcgat cgaggtcgcg gacttccgcc tcgttctcgc ggagctgatg
    15661 cctcacctgc gtaatcgcca gcacattcgt ccccgccgca gtcatcgact gcagcttgtt
    15721 gtgctgccgg gtgatgtgca ccgcaagcac cgcacgctgg tccgccagcg tgccccggaa
    15781 gtaggcagcc tgcaaggaat ccgagccctg cccggccgcc cctgtgggca tgctgggagt
    15841 agccatcggc agaacttagc tgacgacggc gggtcgggct actcgaccga ttcagtcacc
    15901 cgtcagcgtc gactcggccg gcaccgggtg atgcccgttg gtgatcgttg cctccgtcgg
    15961 ccgacacatc tcgatcgcgt tatccgtata ggcccggtag ccgaaatcgg gttgatagcc
    16021 gtggatttcg ggcgtatcga ggtcgcggat gaacgtaagg atctcgcggc tgaacacctg
    16081 tcccggcacc agcaccggga agcccggggg gtagggcgtc acgaacgtcg ccgacaccac
    16141 gtcgataccg gcgtccagct tctcaaagat ctcgtcgctg ctcaggtact cgcagttgga
    16201 atcgttgtag gacaggtaga acgcacgacg aacgtcgccc tcgggggtgt cgtcgatgct
    16261 gctttggtcg cggaagaccg ggtggaagcc gctgaagtct ggctggaccc cggcgcgggc
    16321 cgtcagccgt cgcacccgct gctcgaaccg gctgcgttcg cccagcccca tctccgagat
    16381 gtgctcgtcg agctcgcggg cgatcttcac cagcacctcg accaggaacg ccaccgagct
    16441 gcgggtggtg ccgatgttgg tcatgaacaa cacggtgttg cgtgacgtct tgttgatctg
    16501 cacgccgtgg cgatccatca actgctcgcg cttgaacgtg tcgccgtcgt atcccgtgcg
    16561 gccgatcgac aacgtgatgc gcgaggggtc gagaacgaac tcgtcggcgt cccacgccgc
    16621 catcatgttg cgcagcccag accgcagcgg ctgcgcgatg cccgacgggc ggaactcgtc
    16681 cgggatcaga tccgacgtct tgaggcactc catgtagcgg ctcagcagcg gatggttgtc
    16741 gatcgcgtcc cgcagctgca tggcgttctc gatctggcgc tgcaccagct cgacgccttc
    16801 cagcgcgacc tgcctgcggc caaggtccag cgacgccagt atctggtagt tcggcgacgt
    16861 cgaggtgtgc gccatgtagg cctcgtggaa cggctcggcc accttctggt cgaagtcctg
    16921 gtcgtagacg tggatcatgg agccctgccg cagcgacgtc agggtcttgt gggtggactg
    16981 gggtggcata gacacgcacc cgtgccttgg ccgggtccgg aatcagccgg cgatggagca
    17041 tttcctcgtc gatcggctga ttatcggcat cggcggccag ctgggcttcg aactcacgct
    17101 tgtattcgtt gtcgcccaac ttgtctcgca gattgcgagc cgccgtcatc gcggtccgat
    17161 tgcggtatac cgggtggaac cgggcgaacg cgaaccatgc ttcgtcccag aggaacacca
    17221 gatccggctt gatggccagg cattcctcca tgacgcgctg gacgtcgtag acgatgccgt
    17281 cgaaggtgca gttggtcagt gacatcatct tgacgcggtc cagcttgccc gcgcgtttga
    17341 gtgcaagcag tttcgacttg atctcctgca gcggcaccgc gccgtacatc gaatacgacg
    17401 gcagcgcata cgcttccaga tagatgacct ctgcgccggc cagcatcatg ccgtagtggt
    17461 gcgactggtg gcagttgcgg tcgagcagca cgatgtcgcc gggcgccacc agcgactggg
    17521 tgacgatctt gttggccgtc gaggtgccgt tggtgacgaa gtaggtgtgc cgtgagccgt
    17581 acgcttcggc cgccagctgt tgcgcgtcac gcagcggacc ggtgggctcg agcagcgagt
    17641 cgaggccacc gcacgtggcc gacgtttcgg ccatgaacac gtcgaggccg tagaagccga
    17701 ccatgtcctt gatccagtgc gagttgacga tcgacttgcc ctgcgagatc ggcagcgcgt
    17761 ggagacgccg gtcggccggt ggctgtactg cttgagagcg gagaagaacg gggtgcggta
    17821 gcgcgccgcg acgccctgca ggatcgacag gtgcagctcg agcacgcctt cccgggcgtg
    17881 gaacacccgg cggaaatgct ggccgagccg gcctgcgatg tcctcgacct cgatctcggt
    17941 catcagatac aggtccagct caggccgcag cttcgccagc gaagtggcca ggatctcggc
    18001 gcgctcgtcg ggcgatttgt ggtcgtcgag ttcatcggag atcttggtgt cgacgaactc
    18061 cgcgagcgcc gacaggtcgc ggatcgactg gtgcgagaag cggcgacgga tgacgactgc
    18121 ttgcagattg acgttgagcc gtgcggcgat cagcgcctca tcgccggacg acaccacaac
    18181 gagctcgtag acgaactcgt cgtctggacg acgccagctg cgcacctctt tgcgcagcgc
    18241 gcgctcctgc gcctccgtca tcgactcgac taccagcacc tcgaaatacg gcttgtcgcg
    18301 ttgtgccacg ggctggtgct ccagctgacg cgggtcggtt ccgaacatgt cgtgttcgtc
    18361 caggccggcg tgctcgacgt cgccggtgcg gaacgactcg gtggtcagcg ctcggttgat
    18421 ccgggtaacc gcgtgtgcga acttgtcgta gctacccgcc gagaacagcc gctggaccct
    18481 ggcgaactgc ggcgaacctg gatatgccca gtacggttcg agcggtgtca ggtggtcaag
    18541 agttctctgc agatcgcgac gtactcttcc ctgagctcgc ccttcgtcgt cggccgggtg
    18601 aggcggtcgg cagcctcttc caaccggcac caggcgtcgc tccgcaattg ccatacactg
    18661 ttgtactcac gcgaattatc gctcacagca gaccctttcg tcgggtcaag cgtggcagag
    18721 caattaccac cggcaggcgg gcgctaggcg aacgctatgc gaccatcgcg taacgagggc
    18781 tcgctgggaa gttgcttagt gcgagcgggc gaggtcgggg tggtcgcgct cgttgaatcg
    18841 gcgaaggccc tcgacggttt cgggctccac gatcgaccgg ttgaagcact ccgcctcgat
    18901 gcgcaagccg tcgtcgagcg gccggcccag gcccctgcgt gcggcttcga ggtcggtgcg
    18961 cagcgcgggc tgggggaggg tggcgatggc ctcggcgagc tcgacagccc gctgcaggca
    19021 gctccctttc ggcacgactt cgttgacgag gcctatgcgt tcggcttcgc gggcgtcgat
    19081 cactcgcccg gtgacgatca ggtcgatcgc ccggcggtac ccgaggatgc gggtaagccg
    19141 ttgcgtacca ccgtcggcca gcccgatgtt ccagcgccgg caggtcaccc cgaacgtggc
    19201 gtgctcgtcg gcgatcgcca tgtcggtcca gcacgcccac tccagtccgc ctgcatacgc
    19261 gacgccattg accgccgcga tggtgggctt gtagatgttc gtccaccgcg acgggccgag
    19321 cacaccgtcg gcgggactgg cgtcgacctc gagctccccg gcgaacgccg ccttcaagtc
    19381 accgccggca cagaacgcgt cgtcaccggc accggtgagt acgccgacga acgcgtggtc
    19441 gtccgcccgg aagcggtccc aggcatcgac cagctctcga tgtgtctgcg gcccaatggc
    19501 gttcatgcgc tccgggcggt tgatagtgat gatcgtgacc ggtccgcgct gctcgtagat
    19561 cagatgctca taacgggtca tgcggagtgt gccaatcgcg catcgagagt ggcgtggaag
    19621 cgtccgatcg cgctctcatg cgtgccgaac tcaacaaagg tgttcgcccc ggcgtgcaaa
    19681 ccgcgctgca cgccttcgga catctcgttg tcttccacgc cgccctcgtt gagcaggttg
    19741 cgcgcccccg cggcgtcttc ggggttggcc gacacgcgct cggccacgtg gggcgtgacc
    19801 atggcataga tcgtgacggt ggtgtggtcg acgtcgatcg ggtcgatcgt tatcaccagc
    19861 acctgattcg ggaacgtcgc gaccatgaca ttcgggaaca agtgatacac gtaggtcact
    19921 cggtgaccga cattccaggt ggattctggg cggtcacgca gtcgttcaat attcttgtac
    19981 gggaacgtga ttcgactgtt cggtccgaac gcctccacaa cgttgaggtc gtcgtactgg
    20041 atcggaaaga aggtgtcctt atgcgtcgtg cggatgtgat agccctccag gaactgctcg
    20101 acgagaacct tccagttcat gtcgcgcaga gcaggccgta cggcgaaaag ccgctccgcg
    20161 ggcagtagct tgtcgcgcca tgggctgccg tcggtcagcg ccgccatcgc ggtatcggtg
    20221 tctcgccccg attcgggcga cagcggaccg atgacgatca gcccgtccac ctcacgactg
    20281 tccacctcga ccagtccgcg ggtcggcatg tcgaggtcgg ggaacgcctc gggatggggc
    20341 acgtgcgaca gcgtgccgtc caggcggtac gtccagccgt gatagcggca gaccaacgcg
    20401 tgtgaacagc cgggtccctc gacgagggcc atcccgcggt ggcggcagga attgcggaac
    20461 acgcgtgccc gaccgtcctt gccacgtacc ccgaacaacg gcacaccgaa ggtgacgcgc
    20521 tcggcgtggt cgccggcgtt ggggatcgcc gcggacggca ggtagacgct cggaaacgag
    20581 cgcagcatct cgagctcatg ggtgaatcgg attgggtcca aatagttctg gaccggctcg
    20641 cgccaggcct cgcccacatc ggtcgtgccc gcgtcgatgt gcgcgaagat gcggcgaacg
    20701 atctccgcat cgtcggcgac cgacgccttt gtcggagtgc tcatactgtg cgagtatcac
    20761 actgtgaacg tcacgcgtca acaactttgt gagatgatgg gtcggtgccc atagtcgatg
    20821 agcgccgagc ccacgtcgcc gacctgatcg gcgaccagtc gctgagcgct cgatccgtcc
    20881 tggcgacggc cttactcggc gccgaccaac cccacctcac ggtcggcgag ctcgtcgcga
    20941 tggcctcact tttcggcatc agtgacggcg ccgcccgcac ctgcctgtgg cgcatggtct
    21001 ccaacggcga actcaccagc gacgacggca tgtacgcctt ggccgggggc ctgctcgagc
    21061 ggcgtgaacg ggtcgacgag gcagctcggt tcgccgccac tcggcagtgg gatggcacgt
    21121 gggaattggc ggtcgtctcg ttggaacgcc ggtcggccgc tgaccgtctc gcgctgcgca
    21181 aggccgcatc agcgctgcac ctcgctgaac tacgggaagg cgtctggatt cgaccggaca
    21241 acctcgaccc cgaccgcctg ccgacactgc gcgcagtgct ggaccgacag tgtgtccact
    21301 tccacggcgc cgctagcgac atccccggcg agacggtgag atcgcttttc aatcttgatg
    21361 attggacggc cgacgcgcgg aggttcagcc gcgccgtgca tgacgaggtc gacgcggaag
    21421 ccgttgcggc cgacgatttc gtctattggt tcgcgctgtc agtcgccgtc gtccgccatc
    21481 ttcagctcga ccctctcctg cccgtcgagt tcctgccgga gcaatggcca ggacatgatc
    21541 tgcgcagcgc ctaccgtcgc ttcgacgagg tcttcaaaca acgcctgaac acagcgttcc
    21601 gttgaccgcc cgcgcgctac ctacctggcc tcgagcccga ggatgccgcg catcattcgt
    21661 tcggcgatca gttcatcgcc gcggacgcct gccacttcga tcgcgaggcc ttccaccgcg
    21721 ccgatcgccg ccgtggcgag gatgcgcgcc ggcgggccgg ccgggatcga tccgtctcgc
    21781 cgccccgcgg tgatcaaggc gccaaaccgt agagctagcc ccttaccgat ctcgcccacc
    21841 ttctgcccga ccggatgatc tcggcccgcg aactcgaccc gcagcgccat catgacgcgg
    21901 gcgatatcgc gtctgcagaa cacgatgtga ccgcgcacca gagcgaccag tacgtcgacc
    21961 gcctcaccct tgcgctgcgc gggttgccat acctcccgct cccaactttc agccacccat
    22021 tcgacaaccg ccaacgcgag gtcctccttg ccttggaaca ggtgatagag ggcgcctctg
    22081 gtgtatccgg cctcattggc cacgcgttca aggacgacat tgccgtatcc gtagtgcgaa
    22141 aaccctctag ccgctgcctc gagcagagca gcgcgagtcc gtgctctgcg ctcggcctgc
    22201 gttcgccggc cgcccaccga aacagctcta gatacgtgca tgtatgtatg ttagtctagc
    22261 gacgggagga ggtccggtat gaaactccca aatgccgaag acggcaccac tcaacgggcg
    22321 cgacgatgtt cggtcgagga accgcccgaa gggcacgcgc attacgacta cgcggacgcg
    22381 ttcgaggtga cgctcgctga tggcgatccg cgttcggctg agcaggtggt tcgcgacggg
    22441 ttagcgcgcg caccgtcggc tttgcgggcg gtgatcctga tcgcgcatcg tcgggtactg
    22501 aggctcaatg tgggccccga cttctggccg attgtggcct cagaaccgga catggtccga
    22561 ctcgaggccg caggatcgct gattgccggt gcacttgtcg cgcgccgaat cggcgggcgc
    22621 acagccaggt tgaccacttt cgtcacctac cagggaccgt tcgcgcggat agtctggccg
    22681 atagttgctc cgctgcatcg gcgggtggcc ccgtacctga tggagcgcgc ggctaggccc
    22741 gcagaatcgg tttgagcgcg tcgagcactg ccgcatcctc aatggtcgac ggcaccggct
    22801 catcgcgccc gtcggcgatg ccccgcatgg tcttgcgcaa gatctttccc gaccgggtct
    22861 tcggcagcgc gggaacgaca tcgacgagtt tgaaatacgc taccgcaccg atgttttcgc
    22921 gcaccgcgtc gatcagttcg tcggcaagcc cctcagccgt cgcaccggcc ttgagcacga
    22981 cgaagcctcg cggcacctgg cccttgatct cgtcggcgac gccgatcacc gcgcactcgg
    23041 cgacggccgg atggcctgcc agcaccgcct cgatcgatcc cgtcgacatc cggtgtcccg
    23101 caacgttgat cacgtcgtcg gtgcggccca tcacgaacag gtagccgtcc tcgtcgaaat
    23161 agccgccgtc gccggtgagg tagaagccgt caaacgccga cagatacgac gacacatagc
    23221 ggtcgtcggc accccacaac gtcggcaggg tgccgggcgg cagcggcaac cgcacgcaga
    23281 tcgcgccctc ctcgttcgcg tcgcactccg agccgtccgg gcgcaggatg tgcacgtcgt
    23341 agccgggcat cggcacggtg gcggagccgg gcttgatcgg cagcggttct atgcccatcg
    23401 ggttggcggc gatcgcccag cccgtttccg tctgccacca gtggtccact accgggatgc
    23461 cgagtatgtc cgacgcccag ccgtaggtgc cggggtcgag gcgttcgccg gcctggaaca
    23521 gatacttcag cgcagatagg tcgtacttgc tcaggtgcag gccttcggga tcttccttct
    23581 tgatcgcgcg gatcgccgtc ggcgcggtga acaccgcctt gacgccatgc tcggaggcga
    23641 cccgccagaa cgcgcccgcg tccggggtgc caacgggttt gccctcatag agaacggtcg
    23701 tcgcgcctag catcagcggg ccgtacacga tgtaggagtg gccgacgacc caaccgacat
    23761 cgcttgccgc ccaatacact tcacccggat gcgtgtcgta gatgttgcgc atgctccaca
    23821 gcagcgctac cgcatgcccg ccgttgtcgc ggacgatgcc tttcggcttg ccggtcgtgc
    23881 ccgaggtgta cagcacgtac agcgggtcgg tcgcggcgac gggaaccgcg tcgacgggtt
    23941 gcgcgtcggc catcagctcg tgccagtcgt gatcgcggcc cgccagcggc tcgcaggggc
    24001 gctgctcgcg ctggaggatg acgcacgccg gcggggcgtg ctcggcgact tcgagtgctg
    24061 cgtcgagcat cggcttgtag tcgacggtcc ttgtcggctc gacaccacac gaggccgaca
    24121 cgatgacagc tggttgtgcg tcgtcgatac gcgccgcgag ttcgttcgcc gcgaacccgc
    24181 cgaacaccac cgaatgcacc gcaccaagcc gtgcgcatgc gagcatcgcg attgccgcct
    24241 cgggcaccat cggcatgtag atgacgacgc ggtcgccctt gtcgacgccc aagccgcgta
    24301 gcgctgcggc gaagcgggcc gtctggtcaa gcagctcgcg gtaggtatag gtgcgctgcg
    24361 atccggtcac gggggagtcg tagatcagcg ccggctgctc ggcgcggccg ccgtcgacgt
    24421 ggcgatccag cgcgttggcg caggtgttca gctcgccgtc gggaaaccac cggtagaacg
    24481 gcggattgga gtcgtcgagg atctgttggg gctcgcgggt ccaggtgacg gccttggcgg
    24541 cgtcagccca gaaagccgcg ggatcggaga tgctggcgtc gaagagttcg cggtacccgg
//
LOCUS       DASRJA010000670         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1499827,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000670 DASRJA010000000
VERSION     DASRJA010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499827"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VFC01_30090"
     CDS             complement(<1..580)
                     /locus_tag="VFC01_30090"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HZN83900.1"
                     /translation="MVEFSIIAFLLFILTGGLVDFSIGFYQWNTASKGLQQGARLAAV
                     SDPVDSSLKNWDGLDVSGTVPGGAFPAFTRVCSGATQSCSGGGTFSSVAMETIVYGRG
                     QTTCGVVAADKTPAMCDVFTRVRPNNVIVTYQQTGLGFAGRPGGPVPTITVELTGLTF
                     NFAFLNGLLGFAPIPLPPMKTTATGEDMSTNWN"
     gene            complement(633..1232)
                     /locus_tag="VFC01_30095"
     CDS             complement(633..1232)
                     /locus_tag="VFC01_30095"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HZN83901.1"
                     /translation="MARSMPSSSASSSLVRYRIPSPGTSSSCIDEGRAIMVRKPLLRM
                     QRFGCDREGAALVEFTVVLPFLVFLGLGAFEFGSALYGHHVITTGLRDAARYLARRDD
                     PMAADTAAKQLAVYGEIGGSTKRVSWWSVGDVTVSVSTIANPVDPDTGARTYRGPDPI
                     KIVRVATSATYPGLGLFAVLGMGPTLAINTFHEERVIGD"
     gene            complement(1141..2508)
                     /locus_tag="VFC01_30100"
     CDS             complement(1141..2508)
                     /locus_tag="VFC01_30100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83902.1"
                     /translation="MLIGTAVLAVDASRLSILATTLQKGADALALAGGAELDRKPTAI
                     ARANAAIANMVANQHKFAAASPATIVTSSVRYLKSLPANDISVIDSAHDTADPALARF
                     VEVTVTPQTLNTILPASFFGGAISVTATARAVAGLDAAVCQFTPLFICNPYEGTGISI
                     WDAVSNPTYRRRQLKLQLGAGGTSQYFPGNYGWLDSPNFGSGSNGANALRDALAMVQP
                     QTCFVQNGVSQKTGNISSADAAINTRFDLWSGPYNNAHNNSAYRPAQNVRKGYVPGHG
                     SNGVCNAEATEPNVNKLGQDDSFPHASGRMGTGDWDFDGYWSTNFGSTSKPTDATGVV
                     YSNTNRPARYDVYRHEIAANLTGLAAQSGGATGETGTPACYSGGGLSDEPDRRIIYGA
                     IIDCGGLNVHGNSGGPLAVTVFAKFFLTEPVAADGTIHAELVGFVEPGTVSNTVARDI
                     VQLYR"
     gene            complement(2648..>2849)
                     /locus_tag="VFC01_30105"
     CDS             complement(2648..>2849)
                     /locus_tag="VFC01_30105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83903.1"
                     /translation="AVAHNIAVQTINPWPRYVGNSRIEIDGERLHVGVKRYKANKSIP
                     PRGLSTQTISVTPNNGVSITGN"
BASE COUNT          524 a          863 c          939 g          523 t
ORIGIN      
        1 agttccagtt tgtgctcata tcctcgccag tcgcggtggt cttcatgggc gggagaggga
       61 tcggcgcaaa tcccaagagg ccgttgagaa aggcgaagtt gaaggtgagg cccgtgagct
      121 cgaccgtgat ggtcggtacc ggcccgcccg gccgacccgc gaagccgaga cccgtctgct
      181 ggtaggtgac gatcacattg ttagggcgaa cgcgtgtgaa cacatcgcac atcgccggtg
      241 tcttgtcggc cgcaaccact ccgcaggtgg tctggccgcg gccatagacg attgtctcca
      301 tggcgacgct gctaaaggtg ccgccgccac tgcaggattg cgtcgcgcca ctgcacacgc
      361 gggtaaaggc tgggaatgcg ccgcctggca ctgtgcccga cacgtcgagc ccgtcccaat
      421 tcttcaggct cgagtcgact ggatcggaga ccgcggccag gcgcgcgccc tgctggaggc
      481 ccttgctcgc ggtgttccac tggtagaacc cgatcgagaa gtcgacgagg cccccagtga
      541 gaatgaacaa aaggaaggca atgatcgaga attcgaccat ggaggcacca cgcgcgtcgc
      601 gcctgaagga tcttaaaagg ttgaacattg tctcagtcgc caatcacgcg ctcctcgtgg
      661 aaggtgttga tggctagcgt cggccccatc cccagcaccg caaacaagcc gagcccagga
      721 taggtggcgc tggtcgcaac gcgcacgatc ttgatcggat cggggccgcg ataagtgcga
      781 gcgccggtgt ccgggtccac cgggttggca atcgtgctga cactcaccgt gacgtcgccg
      841 acgctccacc aggacacgcg cttcgtgctg ccaccgatct cgccgtagac cgcgagctgc
      901 ttggccgcgg tgtccgcagc catcgggtcg tcacggcgcg ccaaataccg ggccgcgtcg
      961 cgcagcccgg tggtgatcac gtgatggccg taaagagcgc tgccgaactc gaaggcgccg
     1021 agaccgagaa agacgaggaa cggcagcacg accgtgaact cgaccagcgc ggcaccctct
     1081 cggtcgcaac cgaaccgttg catgcgcagg agaggctttc gaaccatgat agcccgcccc
     1141 tcatcgatac agctggacga tgtcccgggc gacggtattc gataccgtac caggctcgac
     1201 gaagccgacg agctcggcat ggatcgtgcc atcggctgcg accggttcgg ttagaaaaaa
     1261 cttggcaaag acagtgaccg caaggggtcc gccgctgttg ccgtgcacgt tgaggccgcc
     1321 gcagtcgatg atggccccgt agatgattcg acggtcgggt tcgtcactca gtccgccgcc
     1381 gctgtaacag gcaggcgtgc ccgtctcgcc ggtagcgcca ccgctctggg ctgcgaggcc
     1441 cgtcaggttt gccgcgatct catgacggta cacgtcgtag cgagccggcc ggttggtgtt
     1501 ggagtagacg acgcccgtgg cgtccgtcgg cttgctggtg ctaccgaaat tggtcgacca
     1561 gtagccgtca aaatcccaat ccccggtacc catacgaccg ctcgcgtgcg ggaagctgtc
     1621 gtcttggccc agcttgttga cattgggttc ggtggcttcg gcattgcaca caccattgct
     1681 gccgtggccc gggacatatc ccttgcgtac gttttgcgca ggacgataag cggagttgtt
     1741 gtgggcgttg ttgtagggtc cgctccacag gtcgaagcgg gtattgatag ccgcgtcggc
     1801 gctggagata ttgcccgtct tttgcgaaac tccgttctga acgaagcacg tttgtggctg
     1861 caccattgcc agtgcatcgc gcagcgcgtt ggcgccattg ctgccgctgc cgaagttcgg
     1921 agagtcgagc cagccatagt tgcctgggaa gtattggctg gtgccaccgg cgccgagctg
     1981 aagcttcagc tgccgacggc ggtatgtcgg attgctcacc gcgtcccata tcgagatgcc
     2041 ggttccttca tagggattgc agatgaacag cggcgtaaat tgacacaccg ccgcatcgag
     2101 tccggcgacc gcgcgtgccg tcgcggtgac cgagatggcg ccgccgaaga aggaggccgg
     2161 caggatggtg ttcagcgtct gcggcgtgac cgtcacctcg acgaagcgcg ccagcgccgg
     2221 atcggcagtg tcgtgcgcgc tatcgatgac gctgatgtcg tttgccggca gactcttcag
     2281 ataccgcacg ctcgaggtga caatcgtcgc cgggcttgcg gctgcgaact tgtgttgatt
     2341 ggccaccatg ttggcgattg cggcattggc gcgcgcgatg gcggtcggct tgcggtcgag
     2401 ctcggcgccg ccggccaagg cgagcgcatc ggcacccttc tgcagggtgg tcgcaagaat
     2461 gctcagacgc gaggcgtcga ccgccaggac cgccgtgccg attaacaccg gcaggatgat
     2521 ggagacataa atgagaacgg cgccgcgctc gtccctgcca aatcgacaga gcgagcgaga
     2581 ccgacgcgtc tcacatggcc ggcgcttgtg cacgacatga gccaaggcgg aaaggatctc
     2641 cagcggacta gttgccggtg atagacacgc cattgttcgg cgtgacgctg atcgtctgcg
     2701 tggacagccc gcggggcggg atgctcttgt tggccttgta gcgcttgacg ccgacgtgca
     2761 accgctcgcc atcaatctcg attcgcgaat tgccgacata gcgtggccag gggttgatgg
     2821 tctgcacagc aatgttgtgg gccaccgcg
//
LOCUS       DASRJA010000671        18069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_89106,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000671 DASRJA010000000
VERSION     DASRJA010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18069
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89106"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..906
                     /locus_tag="VFC01_30110"
                     /pseudo
     CDS             176..906
                     /locus_tag="VFC01_30110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003919674.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(1104..1382)
                     /locus_tag="VFC01_30115"
     CDS             complement(1104..1382)
                     /locus_tag="VFC01_30115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HZN83904.1"
                     /translation="MTSDWVPSRVPLDPQSGRILDTAQGILIGLRRCRSEEAFQELLV
                     AAQRHQVPVFAMAWALVHLTCGGNESPETFNAEQSAARHEWGRLLAVQ"
     gene            complement(1534..1989)
                     /locus_tag="VFC01_30120"
     CDS             complement(1534..1989)
                     /locus_tag="VFC01_30120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN83905.1"
                     /translation="MSDSHDLPYPEQGLVLTHFLTVRDVARSRDFYADIFGGRVVLEE
                     NPAIVKVANSWIILNPGGGPTPDKPDVILAPRESGDPVSSFLNVRVADIATFYTDAKS
                     KGAQFLTEPLDRKAEIRCYLRDPDGYLIEIGQATGMLHGVYADRHAEDS"
     gene            3121..3486
                     /locus_tag="VFC01_30125"
     CDS             3121..3486
                     /locus_tag="VFC01_30125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83906.1"
                     /translation="MNASQTDSDQYALSTHVAADHGLYHVSIDAGHNHVAYADIRPTS
                     SNIELSIDVATGNPSPRLRSRLIDAVFDLEVMHTPGTLLATLPLGDVDLLDGVARHCV
                     NIHTRAAGHSCLVDGRLLE"
     gene            complement(3602..4957)
                     /locus_tag="VFC01_30130"
     CDS             complement(3602..4957)
                     /locus_tag="VFC01_30130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019929734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HZN83907.1"
                     /translation="MCDTPAAGAVRCHAEIRTDDSLNPLATVGPAGYGPTDLRNAYGI
                     TASGTATIAIVDAYGYANAEKDLATYRAQFGLPPCTSASGCFRKLNQAGQPSPLPPDQ
                     ISWSQETALDLDMASAICPDCKLLLVEANTNSLNDLSTAVTTAGRMGAHAISNSYGAA
                     ESKTFATYEPRYNIPGVAVTVSTGDAGYGVQFPASSPHVVAVGGTSLTSASNSRGWSE
                     TVWNGTGSGCSTVFAKPSWQVDSGCANRTAGDVAAVADPNTGVAVYAPGSTGASAWYV
                     FGGTSAGAPIIAAAYAINGGSVNYAADAYSQATALNDVTAGSNGSCGTYLCTAAGGYD
                     GPTGLGTPKGATAFGGATTSPPPPAGQPTLAVTVSGNGRVVSNPAGINCAGDGLVCSA
                     TYPTGTSVTLTATATNTKRDMFTGWSGGGCAGATPTCTVAMSSNQSVQATFQKQRQPR
                     A"
     gene            5305..5553
                     /locus_tag="VFC01_30135"
     CDS             5305..5553
                     /locus_tag="VFC01_30135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83908.1"
                     /translation="MVTMRAEAAQYLKDAGGLKLVVYGADGREVAAMFVRGSVSYDEE
                     EATFVAVAADGQTAGELFVDDMVGYDENIDAFVIRTAQ"
     gene            complement(5716..8571)
                     /locus_tag="VFC01_30140"
     CDS             complement(5716..8571)
                     /locus_tag="VFC01_30140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN83909.1"
                     /translation="MSNHLAERPTKTDGVAKWIRRLCVPIAIFWVAVAALTNVLVPQL
                     EKVGEEHNVALSSPSSPSLQAFQRIGKVFGEFDSDSAAMVVLEGDQPLGADAHRYYDE
                     LVKRFNEDTKHVQHVQDFWGDPLTAAGSQSPDGKAAYVQVFLSGNQGEALSLESVDAL
                     RDIIAKTQAPPGIHAYVAGSAAQIADQFEVGNESTVFVTLLTVGVIAVMLLITYRSPV
                     TMILALITVLIEMSAARGIVSFLANVGVIGLSTYSTNILTLLVIAAGTDYMIFLLGRY
                     HERRNEGMDREAAFYDMYRGTAHVILGSGLTIAGAVACLYFTRLPYFQSLGIPAAIGV
                     LVALAASLTLAPAVLVIGSRFGLLEPKRKTAKRGWRRIGTAIVRWPGPILIATMTLAF
                     IGLAALSGYKVSYDVSRYMPASAPSNVGYAAAERHFSKARLNPELLMIETDHDLRNPT
                     DMILLERVAKAVFHTDGVAQVQSITRPLGTPLDNTSIPFQISANSAAQINNLPYQQAR
                     AADLQKQVGVINDSINVLRQQYALQQQSSAVTHEQTVAFQQTVVVAQELRDKIANFDD
                     FFRPLRNYFYWEPHCFDIPICWALRSLFDALDGINELTDQLANVSGSIAKLDELQPKL
                     LALIPPQIQNQETNRDLTMTNHATTAGIYDQTAAALQNSTQLGAAYNASKTDDSFYLP
                     PEAFSNPEFQRGLKLFLSPDGKAARMIITHDVDPATPEGISHIDAMRHAAQEAVKGTP
                     LAGSKIYIGGTASTYKDIADMAHYDLIIAGISALCLIFLIMLFITRSLVAALVIVGTV
                     ALSLGASFGVSVLVWQHLFGIDLYWVILPLAVILLLAVGSDYNLLLVSRFKEEIHAGI
                     NTGIIRSMAGSGSVVTAAGLVFAFTMASFIFSDLVVLGQIGTTIALGLMFDTLIVRSF
                     MTPSIAALLGRWFWWPVRVRQRPIPQKFGEDRQLSLF"
     gene            complement(8568..9014)
                     /locus_tag="VFC01_30145"
     CDS             complement(8568..9014)
                     /locus_tag="VFC01_30145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HZN83910.1"
                     /translation="MQRFSIGSRLMRRWMLLVAVFVVALAGFTVYRLNGIFGSKDVTS
                     TPSNAANDIVPFNPKHVVMEVFGPPGTVATITYVDVNAQPQRVDAATLPWAYDTTTTQ
                     PAVFVNVSAQGDSDSIGCRIKIDDVVKDERTVNTLNAFTYCLDKSG"
     gene            complement(9128..9721)
                     /locus_tag="VFC01_30150"
     CDS             complement(9128..9721)
                     /locus_tag="VFC01_30150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN83911.1"
                     /translation="MIRSSDGNADCPWSPREAELLAVTLELLQEHGYDRLTLDAVATT
                     ARASKATLYRRWPTKAELVMAAFIEGTRQVAVDPDTGTLRGDLLAIGEQICAHVSTHA
                     GTIRAVLVEVGRNAELEAIMQEQFLAQRKALMADVLSRAVDRGEIDGAAITEDLWDVL
                     PGYLIYRSVLAGRAPSSRTVRDLVDNVLIPSLTSHKP"
     gene            complement(9766..10782)
                     /locus_tag="VFC01_30155"
     CDS             complement(9766..10782)
                     /locus_tag="VFC01_30155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZN83912.1"
                     /translation="MQVDVMTVPQPLDKVGDLARRTQTAGFSGLLFTETGRTAYLNAA
                     VASQAAPGLELSTGVAVAFPRSPFVTAATAWELQEATGGKFRLGLGTQVRTHVVRRYG
                     MEFERPGPRLRDYVRAVKACFAAFRSGTLDHHGEFYDLDFITPQWSAGPIDAPDPKVD
                     VAAVNPWMLRMAGEVADGVHVHPLGEPGYLARHVVPNVAEGAAKSGRSVSDIAVIVPV
                     MTIVGDNDEERHNERELVRASMSFYGSTPNYAFIWDDAGFEGTTARIREKQKAGDFRG
                     MAAQITDEHIATFATESTWDGLADTLIDKYGEIATRIVLYNALSDQGGFERYGAIARR
                     ISEA"
     gene            10777..11634
                     /locus_tag="VFC01_30160"
     CDS             10777..11634
                     /locus_tag="VFC01_30160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005256475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HZN83913.1"
                     /translation="MHAGTVRRRMFHLAGAVGASALVAACAGGGSTKTDGTSAASTST
                     QSTADAASCLTAAPAETAGPYPGDGSNGPNVLIESGVVRSDIRSSFGAYSGTADGVPV
                     TIDLTMQDITNNCAAGTGMAVYIWHCDRGGDYSLYSQAAANQNYLRGVQVADQNGRVS
                     FTSIFPACYAGRWPHIHFEVFDSLEAAVAGENAQLTSQIALPEQSCTAVFGYEAGYSA
                     SVQNMSQVTLSSDNVFGDGWDAELASVTGDPAGGMTIALTIGVGEKSASTSPNGGPPP
                     GPPPQRPAS"
     gene            complement(11871..13031)
                     /locus_tag="VFC01_30165"
     CDS             complement(11871..13031)
                     /locus_tag="VFC01_30165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HZN83914.1"
                     /translation="MRIGVGVSTAPDPRKAAVEAAEHARDELAGEAPSLGVLLGSRAH
                     TERAVDVLDAVQVIVEPPALIGCVAQAIVAGRHEIEDGPAVAVWLASGLAAETFSLDF
                     VRTGSGGLLAGYRFDRTTHDLYLLLPDPYTFPSNVLVKHLNTDLPGTAVVGGVVSGGL
                     GPHDTRLFRDRDVLTSGAVGVRLPGAHCVPIVSQGCRPIGNAYIVTGADGPVITELGG
                     RPPVQRMREIIEGLHPDEQELVGHGLQIGIVVDERLAAPGQGDFMIRGLLGADPSTGA
                     IEIGEAVEVGATVQFQVRDAAGADKDLRLAVERTRAQLSGRPAGALLFTCNGRGRRMF
                     GIADHDASTIEDLLGGIPMAGFFAAGEIGPIADRNALHGFTASIALFVDDTD"
     gene            complement(13063..15468)
                     /locus_tag="VFC01_30170"
     CDS             complement(13063..15468)
                     /locus_tag="VFC01_30170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN83915.1"
                     /translation="MGSAPGAPADREIVLTDEFQHALALLAEGEHLFLTGKAGTGKST
                     LIRRFMAETDRSVVVVAPTGIAALNVDGYTIHRLFGFRATTTLHDVRRGDYRPGRFTK
                     TLASLDTLIVDEASMVRADVFDMVGAALERFGPSPGQPFGGVQIVLVGDLYQLPPVVT
                     EGESDFFSSTYETPYFFSANSFRRDDFPTVALTTVFRQLGDDRMTAILNEIREGVLLG
                     HAQEQLDARTNPDFVPPDDEFWLTLAPTNRLVSARNRQQLERLPGDEVVHHARVSGDL
                     SLFDPPADDVLRFKVGAQIMMLNNDQGGRWVNGTIGRIVDIDQDRYGLVVAVEFPDGE
                     CEEVTPYTWEATRPVVDGGALRREVVGTFTQLPFKLAWAITIHKSQGQTLDRLIVDLT
                     GGMFSTGQLYVALSRCTSMNGLVLKRTVLPKDLKVDRRIARFLRSAVRDDRVRRYCAI
                     AILTVGEEGRMSRPRPVELAVAFDDGTALSTVVNPQRDLADARAAYGITVSDVLLAPT
                     LREAWAVIAPMLAGYTPVGFGIDETLGLIDFELKRLGHVTAMPLGLQVSGASDTAPAA
                     LGRARAALDAHARSGVDDGGASPFDEPEPSESVSGLLVSRDDDAPTPAADHLPGLSAI
                     LRVSRDVSAIVLRGMSARQVQGLAETPWIAAARQSVADQLCAAASRATLTDAVVERLQ
                     EAGKLLGVDIVSGTCVVERHDIGAALVPGARICFTGTAQDASGRIVERDEMERLAQKA
                     GLAPVKSVTKTRCEVLVTAEAGSQSGKARKAQEYGKPVFPADEFFAWLAGKGIVDAAA
                     SRT"
     gene            15646..16233
                     /locus_tag="VFC01_30175"
     CDS             15646..16233
                     /locus_tag="VFC01_30175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007171417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1942 domain-containing protein"
                     /protein_id="HZN83916.1"
                     /translation="MRIRKLAAPVGVAAVAIGMAAGTVIPASAANNIKPFGQPESLND
                     PATGAPMITYTVHGLNPSSDAVFHNGQLYEAEVTVDGGIPITPMFNARAESGQNYRVI
                     GGGAPGKLYFDVVGDTPNSVVYNDGVQDLLVWVSATPISSGAPDVTGESTEIIGGAPA
                     EAPEAGPAAPEVVPAPEEVNPFETPEQRAAREGGR"
     gene            16399..16827
                     /locus_tag="VFC01_30180"
     CDS             16399..16827
                     /locus_tag="VFC01_30180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1942 domain-containing protein"
                     /protein_id="HZN83917.1"
                     /translation="MKIQKLAAPVTAAAIMISVAVGTASAASNIKPFGQGETLLDPVT
                     GAPTITYTVDGLNPSSDAVPHNGQLYEATVTAQGGMPMVWMFNARAESGANYRVIGGG
                     IPGKLYFDVVGDVPNSVVYNDGVQDLLTWIPGPPEGGTRP"
     gene            16885..17346
                     /gene="cynS"
                     /locus_tag="VFC01_30185"
     CDS             16885..17346
                     /gene="cynS"
                     /locus_tag="VFC01_30185"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HZN83918.1"
                     /translation="MTRNEITQQIIAARLAKGLSWQQLADAIGKPVVWTTAALLGQHP
                     IPAEAGRALVDVLGLDESVVPVLAAVPMRGGLPTAVPTDPTIYRFYEVLQVYGPAIKE
                     LIHEQFGDGIMSAINFSVNVEKKPHPAGDRVVVTLDGKFLPYDWTAADTAG"
     gene            17475..>18069
                     /locus_tag="VFC01_30190"
     CDS             17475..>18069
                     /locus_tag="VFC01_30190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZN83919.1"
                     /translation="MTSTRRVRGACLAAVFAVLAVLGGVVVSTTPDCADHCHTLTAAP
                     VPQAASQPAPPQPAAVWTAPSSGSDHVSPAGPVLVTATAGTITDVTMSSDSGKVIPGV
                     LTPDGTVWKPTTQLGYGRTYTMSITARGPNGMPSRQTSSFTTLTPGNQTEVYLETTAG
                     GLLQDGATYGVGMVVVAKFDEPIDNKANAERHLVVTTN"
BASE COUNT         3318 a         6058 c         5579 g         3114 t
ORIGIN      
        1 atcgaatccc gacccgcacc gccttcccac aaacccgggg tgtcacggag tacggtgatc
       61 ttcgcgatcg tggacctgct tggcaaggcg cagcctcccg ttaccggtcg tgcggattac
      121 tcgggggtgt ccgcgcgggc atcgaagccg acaaggactc cccgcttgag ccgttatggg
      181 gacgacattg tcagcaaaaa cgccgagcct gcggccacac ttcgaggacg tgcaggccca
      241 ctacgacctg tccgatcaat tcttcgagtt gttcttggac ccgacgcgga cgtacagctg
      301 cgcgtacttc gagcgcgacg acatgacgct ggaagaggcc cagatcgcca agatcgacct
      361 ggcgctgggc aaacttggct tgcagcctgg catgacgctg ctcgacgtcg gctgcggttg
      421 gggggcggcg acgctgcggg cgttggacaa atacgacgtc aacgtcgtcg gcctgacgct
      481 gagcaagaac caggctgact acgtcgagca gctactcgca gcatcggata gcccgcgttc
      541 caagcaggtg ctgctcgagg gctgggagca gtttgaggag ccggtggacc ggatcgtggc
      601 gatcggcccg ctcgaacatt tcggctatga ccgctacacc gcttttttca acagggctta
      661 tgacctgttg cccgacgatg gcacgatgct gctgcacacc atcgtgcagt tgagcaccga
      721 agagaacgaa aaactcaaac tgccagtgac gatgcggcat ctgcgctgga ggctcataag
      781 gacgaggcga tcgcgatcca gtccgaagag gtctacgacc ggtacatgaa gtacctcacc
      841 ggctgcgccg agttgtttcg cgacggctac accaatttgt gccagttcac gttggataag
      901 cgatagtccc ggccctcggg gcttattggg gcttcttgtt gcctccggtg caacgatgaa
      961 tccatgatcg acctatcgga gaggtgatcg gacaaccgcc agagccgttt gcgcgaaggt
     1021 gacgatccgt cgaccaaccc tcggtccagg tgaaagccgg gtgaccacag gacgcttcga
     1081 cacgattcat acgacgagtg agttcattga actgccaaca atcggcccca ttcatggcgc
     1141 gcggccgatt gctcggcgtt gaaggtttcc ggcgattcgt tcccgccgca ggtcagatgg
     1201 accagcgccc acgccatggc gaagacgggg acctggtggc gttgggcggc aacgagcagc
     1261 tcttgaaacg cttcttccga acgacaccgc cgaaggccga tcaagatccc ctgcgcggtg
     1321 tcgaggatgc gcccgctctg cgggtctaga gggacccggg agggaaccca atctgacgtc
     1381 atgtctttcc tatctgcaga gcgcggatgc acaaggagcg cgctttagca tcattttgtg
     1441 ccagccagcc gttgggccag accatggcac gtatcggtat gtagccgtga tcgccttgcg
     1501 acagcaccgt ggcaatcgac caacgtgaat gtgctagctg tcctcggcat gcctgtcggc
     1561 gtagacgccg tggagcatgc cggtggcctg tccaatctcg ataaggtatc cgtcggggtc
     1621 gcgcagatag caccgtatct ccgctttgcg gtcaagtggc tcggtaagga attgtgctcc
     1681 tttggatttg gcgtcggtgt agaacgtcgc gatgtcggcg acccgcacat ttaagaatga
     1741 ggaaacagga tcaccagact cgcggggagc caggatgaca tcgggtttgt ccggtgtcgg
     1801 gccgccgccc gggttcaata tgatccagct gttggcgacc ttaacgatgg ccgggttctc
     1861 ctccagcacc acccgtccac cgaagatgtc ggcgtagaaa tcgcgcgacc gcgccacgtc
     1921 gcgcacggtc aggaagtgtg ttagcaccag cccctgttcg ggatagggca ggtcgtgcga
     1981 gtcggacatg attcccctcc atcaagttgg tgttctgcgg cggacgggtc gcccctgcgg
     2041 cgcccgtcca gcggccgagt tcgccgactg cgggcatgtc ttcaaggcta cggtggcgct
     2101 gccgagcgcg agaaaccgat cggccaggtc cagagacaac tgaggactgg acgatctgct
     2161 gccacgaacc gtcgcggtgc tcaacgactc cggcggacac tctgcagggt ccggcgccaa
     2221 tgctgacggg atcgcctgta tccaaactgt gcccaagacg tgcccagatc aggaccgctt
     2281 acgcccgaag atcccgccac aatcgacgcc atgctgcttc agattcacgt tcagccagcg
     2341 cccgaccgat tgtgcgaaac ctgcggtggt ccatcggagt ggatgtcgca gtcattaacc
     2401 ccgggaaacg gcggagcggc gcgcgaaatg tcgccgtgga cggcacggtg cgcggacctg
     2461 tggtgccaga gccgcagacc aaggcaccgt aagacacgcg cgtctgctga ttgctgagcc
     2521 ggtcggtccg ctgggccgac ggtggtgcta gtcacgggtt cgtgtcggtt gcctgtcagt
     2581 tgtgctgttc aggcgcggcg ggagcgggcc ggtcaacgca ctagccgcac actgccgttg
     2641 tctacacctc tgcagccaga ccgccggaaa cgaccaccat gcacaccgat gcacacgacg
     2701 cgagccgcga agagcgccgc gcgcagcttg agcgacgacg cgcagccgtc gcaaaccaat
     2761 tgcgtctcct ggcaatcgaa ttagcggaca tcgacagaca acttgacgag atcaaccact
     2821 cggagcgttg aaagtcacac cgtttgaaca ccggtcggcg gtctcgtcgc ccggagccgc
     2881 ggccgggagt gcgcgtatcc gcagcaaggc gatttcgttc agcacgtccc ggattcggtc
     2941 ggcacggtga gtccaacagt tctatcgcct ggacggcatg atctggcttg ggttaggtac
     3001 ttcctcccgg gcagaaatgg tgggaccgta tctacagacc tccgaagtgt ttaatacgac
     3061 acccgatcgg cgtcaccgct gcggaggcta aatatcaatt gccgcacacc tggagaagcg
     3121 atgaacgcat cccagacgga ctccgaccaa tacgccctct cgactcacgt cgccgccgac
     3181 cacggcctgt accacgtcag catcgacgcc ggtcataacc acgtcgcgta tgccgacatt
     3241 cgtcccacct cttccaacat cgagctcagc attgacgttg ccacggggaa cccgtcccct
     3301 cgtctgcgca gccgcctcat cgacgcggtg ttcgacctcg aggtcatgca cacacccggg
     3361 acattgttgg ccaccttgcc cctcggtgac gtcgacctac tcgacggggt ggcccgacat
     3421 tgcgtcaaca ttcacaccag ggcggctgga catagctgcc tcgttgacgg acgcctcttg
     3481 gaataacgaa ccggccctct catcccgaca acaagtacgg gcgcggattt gcggtccgcc
     3541 tgtcatcggc tgtcgcaggc agacggccct tgagctatgc ccggcgcgac cttgccgaga
     3601 cttaggctct gggctgccgt tgcttctgga acgttgcctg cacggattgg ttggaactca
     3661 ttgcgaccgt gcaggtgggt gtggcaccgg cgcaaccgcc accggaccag ccggtgaaca
     3721 tatctcgctt cgtattggtc gctgtcgccg tgagcgtcac gctggtaccg gtggggtagg
     3781 ttgccgagca caccaaaccg tcgccagcgc aattgattcc agcggggttg ctgacgacgc
     3841 ggccattccc agaaactgtg acagcgaggg ttggttgccc ggcaggtggc gggggcgagg
     3901 tggtggcgcc gccgaaggca gtagcccctt tgggagtgcc caggcccgtc ggcccgtcgt
     3961 atcctcccgc ggctgtgcag aggtaggttc cgcagcttcc attcgatccc gccgtgacgt
     4021 cgttcaaagc ggttgcctgc gaataggcat cggctgcgta gttcaccgaa ccgccattga
     4081 tcgcgtaggc ggcagcgatg atcggcgcgc cggcgctcgt cccgccgaag acgtaccacg
     4141 cgctggcgcc cgttgagccc ggcgcataca cggcgactcc ggtatttgga tctgcgacgg
     4201 ccgcgacgtc gccggcggtg cgattggcgc agccgctatc gacctgccat gacggcttcg
     4261 cgaaaacggt gctgcagccg ctcccggtgc cattccacac agtctcgctc caaccacgcg
     4321 agttgctcgc ggaggtaaga ctcgtgccgc cgacggcgac cacgtgcggt gacgacgccg
     4381 gaaactgaac accgtatccg gcgtcgccag tgctcactgt tacggcgacg cccgggatgt
     4441 tgtacctggg ctcgtatgtc gcaaacgttt tggactcggc cgcaccgtag ctattgctga
     4501 tcgcgtgtgc gcccattcgc cccgccgtcg tgaccgctgt gctcaggtcg ttcaaactgt
     4561 ttgtgttggc ctcgaccaag agcagtttgc agtccgggca gatagcgctg gccatgtcca
     4621 ggtcgagtgc cgtctcctgg ctccacgaaa tctggtccgg cggcaggggt gacggctgtc
     4681 ctgcctgatt gagctttcga aagcacccgc tagcggatgt acaaggcggc agcccgaatt
     4741 gcgctcgata tgtggccagg tccttttcgg cgttcgcgta cccataggca tccacgatcg
     4801 cgatagtcgc tgtcccactt gcggtgatgc cgtaggcgtt ccgcaggtct gtcgggccgt
     4861 agccggcggg ccccacagtc gcaagtgggt tgaggctatc gtccgtacgg atctccgcat
     4921 ggcaccgcac ggcacctgcg gcgggcgtgt cacacacgtg tttcgacttg cccgggggca
     4981 acggaggcgg agccgcgtcc gctggtgggg cggccaagac cgcacaacac aagacaacgc
     5041 acaccgcatg gaatagcttg cgcacaacgg ccccccaaca gtccaacgaa tcgttttctg
     5101 gatagtgcac acccaaagtc acaacgacag gcacagcgct gcagtcgcaa cacgatcgac
     5161 accggaaggt gacccatcga ggcatctcgg cagctgaaac gccgccatcc ataccaagtt
     5221 tcgagaattg gtatggcgtg tttcgcggcg tggtttcggg aataccctgt ggaaccaaag
     5281 ccattggcag agaggaatcc agtaatggtc acgatgcgtg ccgaagcggc gcagtacctc
     5341 aaggatgcag gaggcttgaa gcttgtcgtt tacggagcgg atgggcgcga ggtcgcggcg
     5401 atgttcgtgc gaggttcggt gtcatacgac gaggaggagg ccaccttcgt tgcagtcgcg
     5461 gcggacggcc agacggctgg agaactattc gtcgatgaca tggtcggtta cgacgagaac
     5521 atagacgcct tcgtcatccg caccgcgcaa tagcatcacg ccccagcctc gcccgagagg
     5581 acgcagcaca ggtgcgcaac gcctatgcct ggttgatggc cgcggacacg ggctcggaat
     5641 gcaagcggga cctcaaggat ctcgacgccg cgcgtgctcg ctctggcccg ttagtcgcgg
     5701 gcgtcgaaga ggcgactaga acagcgacaa ctgtcgatcc tcgccgaact tctgaggaat
     5761 cggccgctgg cgcacccgca ccggccacca gaaccaccgt ccgagcaacg ccgcgatcga
     5821 cggcgtcatg aacgaccgga cgatcagcgt gtcgaacatc aggcccagcg cgatcgtcgt
     5881 gccgatctgg cccagcacga ccaggtcgct gaagatgaac gacgccatgg tgaacgcgaa
     5941 caccagcccc gccgcagtca ccaccgatcc gctgcccgcc atcgaccgga tgatgccggt
     6001 gttgatgccg gcatggatct cttccttgaa ccgggagacc agcagtaggt tgtagtccga
     6061 ccccaccgcc agcagcagga tgaccgccaa aggcaatatc acccaataca agtcgatgcc
     6121 gaacaggtgc tgccacacca acaccgacac accgaacgac gcgcccaacg acaacgccac
     6181 cgtgcccacg atcaccagag cggcgacgag gctgcgcgtg atgaacaaca tgatcaggaa
     6241 aataaggcag agcgccgaga tgcccgcgat gatcaggtcg tagtgcgcca tgtctgcgat
     6301 gtccttgtag gtggacgccg taccaccgat gtagatcttc gaccccgcca acggcgttcc
     6361 cttgaccgcc tcctgggcgg cgtgccgcat cgcatcgatg tgcgaaatac cctcgggcgt
     6421 agccggatcc acgtcatgcg tgatgatcat gcgcgcagcc ttgccgtccg gcgacaggaa
     6481 cagcttcagc ccgcgctgga actccgggtt gctgaacgcc tccggcggta gatagaagga
     6541 gtcgtcggtc ttcgacgcgt tgtacgcggc acccagctgc gtggagttct gcagcgccgc
     6601 cgcggtttgg tcgtagattc ccgccgtggt ggcgtggttc gtcatcgtca aatcgcggtt
     6661 ggtctcttga ttttggatct gcggcgggat cagcgcgagc agcttcggct gcagctcatc
     6721 cagtttggcg atactccccg aaacgttggc caactgatcg gtcaactcgt tgatgccgtc
     6781 gagtgcatcg aagagggatc gcagcgccca gcagatcggg atgtcgaaac agtgtggctc
     6841 ccagtagaag tagttgcgca gcggccggaa gaagtcgtcg aagttggcga tcttgtcgcg
     6901 caactcctgc gccacgacga cggtttgttg gaatgccacg gtctgttcgt gcgtcacggc
     6961 gctggactgc tgctgcagtg catattgctg tcgcaggacg ttgatcgagt cgttgatgac
     7021 acccacctgc ttttgcaggt cggcggcgcg ggcctgctgg tagggcaagt tgttgatctg
     7081 cgccgcactg ttcgcactga tctgaaacgg gatcgacgtg ttgtcaaggg gcgtgcccag
     7141 cggccgcgtg atcgattgca cctgagcgac gccatcggtg tggaacacgg ccttggccac
     7201 ccgctccaac aggatcatgt cggtgggatt tcgtagatcg tggtcggttt cgatcatcag
     7261 cagttcggga ttcagccgcg ccttcgagaa gtgacgctcc gcggcggcgt atcccacatt
     7321 ggacggggca ctggccggca tgtaccggct gacgtcgtaa ctcaccttgt agccggatag
     7381 cgcggctaat ccgatgaagg ccagcgtcat cgtggcgatc aggatcggcc ccggccatcg
     7441 aacgatcgcc gtcccgatcc gccgccagcc tcgcttggcc gtcttgcgct tgggctccag
     7501 cagtccgaag cggctgccga tcaccagcac ggcgggcgcc agcgtcaacg aggcggccag
     7561 cgcgacgagc acaccgatcg ccgccgggat gcccaggctc tggaagtacg gcaaccgggt
     7621 gaagtacagg caggccaccg cgccggcgat ggtcagcccg gagccgagta tcacgtgcgc
     7681 ggtcccgcgg tacatgtcgt agaaggcggc ctcccggtcc atgccttcgt tgcgtctctc
     7741 gtgatagcgg ccgagcagga agatcatgta gtcggtaccg gcagcgatga ccagcagcgt
     7801 gaggatgttc gtcgaatacg tcgacaggcc aatgactccg acgttcgcca ggaacgagac
     7861 gatcccgcgt gcggctgaca tctcgatgag caccgtgatg agcgccagga tcatggtgac
     7921 cggcgagcga taggtgatga gcaacatcac cgcgatcacc ccgacggtca acagggtgac
     7981 aaacacggtg ctttcgttgc ccacctcgaa ctgatctgca atctgggctg ccgagccggc
     8041 gacgtaggcg tggattcccg gcggtgcctg cgtcttggcg atgatgtcgc gcagcgcgtc
     8101 gaccgactcc aacgacaacg cctcgccctg gttgccggac aggaacacct ggacgtacgc
     8161 ggccttcccg tcggggctct gcgagcccgc ggcggtgagc gggtcgcccc agaagtcctg
     8221 tacgtgctgg acgtgcttgg tgtcctcgtt gaaccgcttg accaactcgt cgtagtagcg
     8281 gtgcgcgtcg gcaccgaggg gctggtcacc ctcgagcacc accatcgcgg cactgtcgga
     8341 atcgaactca ccgaacacct tgccgatgcg ctgaaacgcc tgcagcgacg gcgaactggg
     8401 ggagctcaac gccacgttgt gttcttcacc gactttttcg agctgcggca ccagcacgtt
     8461 cgtcaaggcg gcgacggcca cccagaagat cgcgatcggc acgcagagcc gacgaatcca
     8521 cttggcgacc ccgtccgtct ttgttgggcg ttcggcgagg tggttgctca tccggacttg
     8581 tccaagcagt aggtgaaggc gttcaacgtg ttgaccgttc tctcgtcctt gacgacgtcg
     8641 tcgatcttga tccggcagcc gatcgagtcg ctgtcgccct gggcggagac gttgacgaag
     8701 acggccggtt gggtggtggt tgtgtcgtag gcccacggca gcgtcgcggc gtcgacccgc
     8761 tgtggctggg cgttcacgtc cacgtaggtg atggtcgcga ccgtgccagg ggggccgaat
     8821 acctccatga cgacgtgctt cgggttgaac ggaacgatgt cattcgcggc gttactcgga
     8881 gtggacgtca cgtccttgga gccgaagatg ccgttcagcc ggtacaccgt gaagcccgcc
     8941 agggcgacga cgaacaccgc gaccagaagc atccaccgtc gcatcaaccg gcttccgatc
     9001 gaaaaccgct gcacccgtgg ccctttcgac gagcggtgac acgttgacgg acggtacgac
     9061 acctcgccag ccaatatcaa ctaggttgac agtacggtac gggaccggac cggacaaccc
     9121 gtacgcctca gggtttgtga ctcgtgaggc tggggatgag cacgttgtcg acgaggtctc
     9181 gcaccgtacg gcttgacggg gcccggccag cgaggaccga gcggtagatg aggtagcccg
     9241 gcagcacatc ccacaggtcc tcggtgatgg cggcgccgtc gatctcgccg cggtcgacgg
     9301 cccgggagag gacgtctgcc atgagcgcct ttctctgggc gaggaattgc tcctgcatga
     9361 tggcctcgag ctcggcattc cggccaacct cgacgagcac cgcccgaatg gtgcccgcgt
     9421 gggtgctgac gtgagcgcag atctgctcgc ctattgccaa cagatcgccg cgaagcgtgc
     9481 cggtgtccgg gtcgaccgcg acctgccggg tgccctcgat gaacgccgcc atcaccagtt
     9541 cggccttggt cggccatcgt cggtagagcg tggccttgct cgcgcgtgcc gtcgtggcca
     9601 ccgcgtcgag ggtcaaccgg tcgtagccgt gttcctggag cagctcgagc gtcaccgcga
     9661 gcaactcggc ctcccgcggc gaccacgggc aatccgcgtt gccatcggaa ctccggatca
     9721 cgggaacctc catggagcca gtcaacttgt gcgaacgagt atcggtcatg cttccgatat
     9781 gcgccgcgcg atggcgccat atcgctcgaa gcccccctga tctgagagtg cgttgtaaag
     9841 cacgatgcgc gttgcgattt ctccgtactt gtcgatcagc gtgtcggcca agccatccca
     9901 cgtcgactcc gtggcgaacg tcgcgatgtg ttcgtcggtg atctgcgcgg ccatccccct
     9961 gaagtcgccc gccttctgct tctcccggat acgcgccgtc gtcccctcga accccgcgtc
    10021 gtcccagatg aacgcgtagt tgggcgtgct cccatagaag ctcatactgg ctcgcaccag
    10081 ctcgcgctcg ttgtgccgct cctcatcgtt gtcacctacg atcgtcatca ccggcacaat
    10141 caccgcaatg tccgacaccg accgccccga cttcgccgct ccctcagcca cattcggcac
    10201 gacatggcgc gcgagataac ccggttcacc cagcggatgt acatgcacgc cgtccgcgac
    10261 ctcgcccgcc atccgcagca tccacggatt caccgccgcc acatcgactt tcggatccgg
    10321 cgcgtcgatc ggccccgcac tccactgcgg cgtgatgaag tccaggtcat agaattcgcc
    10381 gtggtgatcc agcgtcccgc ttcggaatgc cgcgaaacac gccttcaccg cccgcacata
    10441 gtcccgcagc cgcggacctg gacgctcgaa ctccatcccg taccgcctca ccacatgcgt
    10501 gcgcacctgc gtgccaagac ccaggcggaa cttcccgcct gtcgcctcct gcagttccca
    10561 cgccgtcgcc gccgtcacga acggactccg cggaaacgcc accgcgacac ccgtcgacag
    10621 ttccagtcct ggcgcggcct gcgatgccac cgccgcgttg agatacgcgg tacgacccgt
    10681 ttcggtgaac agcagaccgg agaagcccgc ggtctgcgta cgccgggcga ggtcgccgac
    10741 cttgtccaac ggctgcggaa ccgtcatcac gtcgacctgc acgctggaac agtacgccgc
    10801 cggatgttcc acctcgccgg tgccgtgggc gcctccgctc ttgtggcggc ctgcgcaggc
    10861 ggcggctcca ccaagaccga cggcacctcc gctgcgagca cctcaaccca gagcacagcc
    10921 gatgcggcca gctgcttgac cgccgcgcca gccgagaccg cgggccccta tcccggtgac
    10981 gggtcgaacg ggccgaacgt tctcatcgag tcgggcgttg tccgcagcga catccgatcg
    11041 agcttcggcg cctactccgg cacagccgac ggtgtccccg tcacgatcga cctgacaatg
    11101 caggacatca ccaacaactg cgccgctggc accggcatgg cggtgtacat ctggcactgc
    11161 gaccgcggtg gcgattactc gttgtacagt caggccgccg cgaaccagaa ctacctgcgc
    11221 ggggtccagg tcgctgatca gaacggccga gtgtcgttca cgtcgatctt ccccgcttgc
    11281 tacgccggcc ggtggccgca catccacttc gaggtgttcg attcgctcga agccgccgtc
    11341 gccggcgaaa acgcccagct gacttcacaa atcgcgctgc cggagcagtc gtgcaccgcg
    11401 gtgttcgggt acgaggcggg ctactcggcc agcgtgcaga acatgtcgca ggtgacgctg
    11461 agctccgaca acgtcttcgg cgacggttgg gacgccgaac tcgccagcgt gacaggcgat
    11521 ccggccggcg gcatgaccat cgcactcacc atcggcgtag gtgagaaatc ggccagcaca
    11581 tcgccgaatg gcggtccacc gcctgggccg ccgcctcagc gtccggcgag ctgagcttgg
    11641 acgcagactt ctcgtgtgga cgacttcatt gtggacgctc atcgtcgcgt ggaatgcgga
    11701 cggcttgcca acagcgccga cccttgaggg acaacgattc ccgtcacacg gcgaccggcc
    11761 tgtcgctgaa aagtcgctac gagctgggca tttcgcatag tgcaccgcca actgcaactg
    11821 gtgttacaga cgtgacccac acaccggcgc accagcgtcg aggcagaggc tcagtccgtg
    11881 tcgtcgacaa acagcgcgat cgacgcggtg aatccatgca acgcgttgcg gtccgcgatc
    11941 gggccgatct ccccggcggc gaagaaacca gccatcggaa tcccgccaag taagtcctcg
    12001 atcgtcgacg catcgtggtc ggcgatcccg aacatccgtc gcccacgccc gttgcaggtg
    12061 aacaacagcg cgccggccgg acgcccggac agctgagccc tcgtccgctc cacggccagc
    12121 cgcaggtcct tgtcagcccc cgccgcgtca cgcacctgga actgcaccgt cgcgccgact
    12181 tcgacagcct cgccgatctc gatcgccccg gtcgaggggt cggcgccgag aagcccgcgg
    12241 atcatgaagt cgccctgacc cggagccgcc aggcgctcgt cgacgacgat cccaatctgc
    12301 agaccgtggc cgaccaattc ctgctcgtcg gggtgcaacc cctcaatgat ctcgcgcata
    12361 cgctgcaccg gcggtcggcc gcccaactcg gtgatcactg ggccgtccgc gccggtgacg
    12421 atgtacgcat tgccaatcgg ccggcagccc tgcgacacga tcggcacaca gtgcgcgccg
    12481 ggcaggcgca caccgactgc gccggaggtg agcacgtcgc ggtcgcggaa cagccgggtg
    12541 tcatggggtc ccagcccacc gctcaccacg ccgcccacca ccgcagttcc cggcaggtcg
    12601 gtgttgaggt gcttgacgag cacgttcgac gggaatgtgt acggatccgg cagcagcaga
    12661 tagagatcgt gcgtggtccg gtcgaaccgg tacccggcga gcagaccacc cgagccggtg
    12721 cggacgaagt ccaacgagaa cgtctcggcg gccagacccg acgccagcca caccgccacg
    12781 gcgggcccgt cctcgatctc gtggcggccg gcaacgatcg cctgggccac gcaaccgatc
    12841 agcgccggcg gctcgacgat cacttgcacc gcgtcgagaa cgtccacagc ccgctcagtg
    12901 tgcgctcgtg atccgagcag cacacctagc gacggcgcct cgcccgcaag ctcgtcgcgc
    12961 gcgtgctctg cggcctccac cgcggccttc cgtggatcgg gcgcggtgga aacgccaact
    13021 ccgatccgca cacttccatg atgcgcctcg ataagccgat tgctacgtcc tcgacgccgc
    13081 cgcgtcgacg atccccttgc ccgcgagcca cgcgaagaac tcgtccgccg gaaagaccgg
    13141 cttcccatac tcctgagcct tgcgtgcctt tccggactga ctgcccgcct ccgccgtcac
    13201 cagcacctcg caccgtgtct ttgtcaccga cttgaccggc gccagaccag ctttctgcgc
    13261 cagccgctcc atctcgtcgc gctcgacgat ccgcccgctg gcgtcctgcg ccgtgccggt
    13321 gaaacagatt cgtgctcccg gaaccagggc cgcgccgatg tcgtgacgct ccactacgca
    13381 agtaccagag acgatgtcga cgccgagcag cttgccggcc tcctgcagcc gttccaccac
    13441 cgcatcggtc agcgttgccc gggacgccgc cgcgcataac tgatcggcaa ccgattgtcg
    13501 cgccgccgcg atccacggcg tctcggcaag accctgcact tgccgggccg acattcctcg
    13561 caacacgatc gcgctgacat cccggctcac ccgcagtatc gccgacaagc cgggcagatg
    13621 gtcggccgct ggcgtcggcg catcgtcatc gcggctgacc agcagccccg ataccgattc
    13681 agatggttcc ggttcgtcga atggcgacgc accgccgtcg tcaacgcccg atcgggcatg
    13741 cgcgtccagt gcggctcgtg cccgccccag cgcggcaggc gccgtatctg aagctccaga
    13801 aacttgtagc cccaacggca ttgcggtcac atggcccagt cgtttcaact cgaagtcgat
    13861 caggcccagc gtctcgtcga tgccgaaccc gaccggtgtg tatcccgcca gcatcggcgc
    13921 gatcaccgcc caggcctccc gcagcgtcgg tgccaacagc acgtcggaca ccgtgatccc
    13981 atacgccgca cgagcatccg ccaaatcgcg ctgcggattg accaccgtgg acagcgccgt
    14041 cccgtcgtca aaggccaccg ccaactccac cggccgcggc cgcgacatcc gcccctcctc
    14101 gccgaccgtg aggatggcga tcgcgcaata ccgccggacc cgatcatcgc gcaccgcgct
    14161 ccgtaggaac cgcgcgatcc ggcggtcaac cttcaaatcc ttcggcagca cagtccgttt
    14221 cagcaccagc ccgttcatcg acgtgcaccg gctgagcgcg acatacagct gaccggtcga
    14281 gaacatcccg cccgtcaggt ccacgatcaa ccggtccagc gtctggccct ggctcttgtg
    14341 gatcgtgatc gcccacgcca gcttgaacgg caactgcgtg aacgttccga ccacctcacg
    14401 gcgcagggcc ccgccgtcaa ccaccgggcg cgtcgcctcc cacgtgtacg gggtgacctc
    14461 ctcgcactcc ccgtcgggga attcgacggc gacgacgagc ccgtagcggt cttggtcgat
    14521 atcgacgatc cggccgatcg tgccgttgac ccagcgacca ccctggtcgt tgttgagcat
    14581 catgatctgc gcgcctacct tgaaccgcag gacgtcgtcg gcgggcggat cgaacagcga
    14641 cagatcgcct gatacccgcg catggtggac gacttcgtca cctggcagcc gctcaagctg
    14701 ctgacgattg cgcgccgaca ccagccggtt cgtcggcgcc agcgtcagcc agaattcgtc
    14761 gtcgggcggt acgaagtcgg gattcgtgcg ggcgtcgagc tgttcttggg catggcccag
    14821 cagcactcct tcgcgaatct cgttgagaat ggcggtcatt cgatcgtcac caagctgccg
    14881 aaacaccgtc gtcaacgcca cggtggggaa gtcatcacgc cggaaactgt tggcggagaa
    14941 gaaatacggc gtctcatacg tcgacgagaa gaagtccgat tcaccttcgg tgaccactgg
    15001 cggcaactgg tacagatcac caacaagcac gatctgcacg ccgccgaacg gctgacccgg
    15061 cgacggccca aaccgctcca gcgccgcgcc caccatgtcg aatacgtccg cccgcaccat
    15121 cgacgcctca tcgacgatca acgtgtccag cgacgccagg gttttcgtga accgtcccgg
    15181 ccgatagtcg ccacgacgca cgtcgtgcag cgtcgtcgtc gcgcggaacc cgaacagccg
    15241 gtgaatcgtg taaccgtcga cgttgagcgc ggcgatgccg gtgggtgcga ccaccacgac
    15301 gctgcggtcg gtctcggcca tgaagcggcg gatcagcgtc gacttaccgg tccccgcctt
    15361 ccctgtcagg aacaggtgct cgccttcggc cagcagcgcc agcgcgtgtt ggaactcgtc
    15421 ggtgaggacg atctcccggt cggcgggagc gcctggtgca gatcccaccc gacaaacgta
    15481 gctgcgcccg ggcccctagc cgacgttgcg aacggacgca ccgtcacgac gctgacggac
    15541 gggttgcatt gacaccggac gaggtcgaag cattttgcgt tttgaatcgg agtcgccggg
    15601 gtatgttttc gcttcgtgga tagcgtcgaa acgaggggag caagcgtgag gatccggaaa
    15661 ctagcggcac ctgttggggt cgccgcggtc gcgatcggta tggctgccgg cacagtcatc
    15721 cccgcatcag cggccaacaa catcaagccg ttcggtcagc cggagtcact gaacgatccc
    15781 gctaccggtg ccccgatgat cacttatacg gttcacgggc tgaatccgag ttcggacgcc
    15841 gtgttccaca acggccagtt gtacgaagca gaagtgacag tcgacggggg cattcccata
    15901 accccgatgt tcaacgcccg cgctgagagc gggcagaact accgcgtcat cggcggagga
    15961 gcgcccggca agctctactt cgacgtcgtg ggcgacactc cgaacagcgt cgtctacaac
    16021 gacggtgtcc aggatctgct tgtttgggtc tcagcgacgc cgatctccag tggtgccccg
    16081 gacgtgacag gcgagtcaac cgagatcatc ggcggcgcac ccgctgaggc ccctgaagct
    16141 ggacccgccg cccccgaggt cgtcccggct cccgaggaag tcaacccctt cgagactcct
    16201 gaacaacgcg cagcgcgaga gggtggccgc taagcgctag cccagttgtc gctcggtacg
    16261 cgggcggcct gacgaactgc cgaccccttt tcatacagca gaccgccgaa gcgaccggcg
    16321 cgcggattgc gtttccgacc tcggttctgc gggtaccggt tgcggtatac accaggctga
    16381 acgaggggag tcacggtgat gaagatccag aaactagcgg cgcctgtgac cgccgccgcg
    16441 atcatgatca gcgttgctgt cggcacggcg tcggcggcca gcaacatcaa gccattcggc
    16501 cagggggaga ccctgctgga tcccgtcacc ggcgccccga caatcactta cacggttgac
    16561 gggctgaatc cgagttccga cgcggtgccc cacaacggcc agttgtatga agcaaccgtg
    16621 accgcccagg gcgggatgcc catggtctgg atgttcaacg cccgcgctga gagcggggcc
    16681 aactaccgcg tcatcggcgg cgggatcccc ggcaagctct acttcgacgt cgtgggcgac
    16741 gtgccgaaca gcgtcgtcta caacgacggg gttcaggatc tcctgacttg gattcccggc
    16801 cctcccgagg gtggtacacg cccctagcgc tacggcagct gtatctggtg cgcgccgacc
    16861 gcctggtgcg ccgatgatgg ctgcatgact cgaaacgaga tcacgcagca gatcatcgcc
    16921 gcccggctag ccaagggcct gagctggcag caactcgcgg acgccatcgg caagcccgtc
    16981 gtctggacca ccgccgccct gctcggccag cacccgatcc cggccgaggc gggtcgcgcg
    17041 cttgtcgacg tccttggcct cgacgaatcc gtcgtgccgg tgctcgccgc cgtgcccatg
    17101 cgcggcggtc ttcccaccgc ggtgcccacc gacccgacca tctaccgctt ctacgaagtc
    17161 cttcaggtct atggccccgc catcaaggag ctcatccacg agcagttcgg cgacggcatc
    17221 atgagcgcga tcaacttcag cgtcaacgtc gagaagaagc cgcacccggc cggcgaccgt
    17281 gtcgtcgtca ccctcgacgg gaagttcctg ccctacgact ggacggctgc ggacacggcg
    17341 gggtgaagca ctgaattcgc ccccgcaaca agacgattac gaatccgcac agctcagccg
    17401 agttgcgcgg accccaagac gctctgtcta atattgatcc gtcccggccc tcgccgacct
    17461 acaacggaga acagttgacg tctactcgcc gtgttcgcgg tgcctgcctt gcggccgtat
    17521 tcgccgtcct tgcggtgctc ggcggcgtcg tcgtctccac cactcccgac tgcgccgacc
    17581 actgccacac gctgaccgcc gcccccgttc cgcaagcggc atcgcagccc gcgcctccgc
    17641 agcctgccgc ggtgtggacc gcaccgtcgt cgggctcaga tcacgtcagc cccgcgggtc
    17701 ccgtactggt cactgccacc gccggcacga tcaccgacgt cacgatgagc agcgactccg
    17761 gcaaggtgat ccccggcgtg ctcacccctg acgggacggt gtggaagccg acgactcaac
    17821 tcggctacgg ccgcacctac accatgagca ttaccgctcg cgggcccaac ggcatgccgt
    17881 cgcggcagac gtccagcttc actacgctga cccccggcaa ccagaccgag gtgtatctgg
    17941 aaacgaccgc cggaggcctg ctgcaggacg gcgcgacata cggcgtcggc atggtcgtcg
    18001 tcgcgaagtt cgacgaaccc atcgacaaca aggccaacgc cgaacgtcac ctcgtcgtca
    18061 ccaccaacc
//
LOCUS       DASRJA010000672         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4058593,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000672 DASRJA010000000
VERSION     DASRJA010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4058593"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1545)
                     /gene="murA"
                     /locus_tag="VFC01_30195"
     CDS             complement(292..1545)
                     /gene="murA"
                     /locus_tag="VFC01_30195"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZN83920.1"
                     /translation="MSERFVVTGGNRLSGEVAVGGAKNSVLKLMAAALLAEGTSTITN
                     CPDILDVPLMAEVLRGLGATVELDGGVVRVTSPDEPKYDADFAAVRQFRASVCVLGPL
                     VGRCKRAKVALPGGDAIGSRPLDMHQAGLRQLGARCNIEHGCVVAEAEHLRGAEIQLE
                     FPSVGATENILMAAVVAEGVTTIHNAAREPDVVDLCTMLNQMGAQISGAGTPTMTITG
                     VDRLYPTEHRVIGDRIVAATWGIAAAMTRGDISVTGIDPQHLQLVLHKLHDAGATVTQ
                     TDDGFRVVQYERPKAVNVATLPFPGFPTDLQPMAIALASIADGTSMITENVFEARFRF
                     VEEMVRLGADARTDGHHAVVRGIPQLSSAPVWSSDIRAGAGLVLAGLVADGDTEVHDV
                     FHIDRGYPLFIENLISLGAEIERIA"
     gene            1617..2183
                     /locus_tag="VFC01_30200"
     CDS             1617..2183
                     /locus_tag="VFC01_30200"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZN83921.1"
                     /translation="MAVHLTRIYTRTGDDGTTGLSDFSRVSKNDARLAAYADCDETNA
                     AIGVAIALGNPDEQIRKVLQQIQNDLFDAGADLSTPVVENPEHPPLRITQSYIDRLES
                     WCDEFNEPLPALNSFVLPGGTPLSALLHVARTVARRAERSAWDAVNTHKDSVSVLPAK
                     YLNRLSDLLFILSRVANPDGDVLWQPGG"
     gene            complement(2229..2675)
                     /locus_tag="VFC01_30205"
     CDS             complement(2229..2675)
                     /locus_tag="VFC01_30205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2550 domain-containing protein"
                     /protein_id="HZN83922.1"
                     /translation="MSASMMVMVALVGVLAVFSVALSYRLWKLRQVGGTAAILRDVPA
                     VGGHGWRHGVMRYRGGEAGFYRLSSLRWWPDRRLSRRGLEIVSRRSPRGDEFDIMTDA
                     IVILELRDTSPERRRGYEVALDRGALTAFQSWLESRPSPRARRRTF"
     gene            complement(2687..3052)
                     /locus_tag="VFC01_30210"
     CDS             complement(2687..3052)
                     /locus_tag="VFC01_30210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HZN83923.1"
                     /translation="MAELNIDIVAVDREIWSGQGTFLFTRTTSGEIGILPNHIPLVAQ
                     LVDDAMVRVEREGEDDLRIAVDGGFLSVTEHGVTVLAESAEFDSEIDEAAAQRDAESD
                     DPATAAKGRARLRALGQID"
     gene            complement(3082..>3808)
                     /locus_tag="VFC01_30215"
     CDS             complement(3082..>3808)
                     /locus_tag="VFC01_30215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922237.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZN83924.1"
                     /translation="NIFRFTQAGSEVSTLLGRMPSAVGYQPTLADEMGELQERITSTR
                     GRSITSMQAVYVPADDYTDPAPATTFAHLDATTELSRAVFSKGIFPAVDPLASSSTIL
                     DPSVVGDEHYRVAQEVIRILQRYKDLQDIIAILGIDELSEEDKQLVQRARRIERFLSQ
                     NMMAAEQFTGQPGSTVPVKETIEAFDRLTKGEFDHVPEQAFFLIGGLEDLAKKAKSFG
                     VELEGGGKNNGSDKSDEGEKKDD"
BASE COUNT          726 a         1273 c         1156 g          653 t
ORIGIN      
        1 gagaaactga tcaaagcaag acaccaaaaa ctggcatcaa aaaacaccgc accccaacac
       61 ggggagtcag aatgcggcaa aaaacaacaa caaaacaaaa accaccaaac acactattga
      121 gttctcaaac aacacacccg cgctgcaggc aaccctgcca ctgtactcca cttggacaga
      181 gacgtcaaac cgcgtcctcc agtcctattg gcctggggcc gttggcttct cagtaaagag
      241 tacgcctttg tcgggctcga ccgaaaggcg gcctcgcacg cctcctggcg tctacgcgat
      301 gcgctcgatt tctgcgccca gactgatcag gttctcgatg aacagcgggt agccgcgatc
      361 gatgtggaac acgtcgtgaa cctcggtgtc gccatcggcg accaggccag ccagcaccaa
      421 tcccgcgcca gcccggatat ccgacgacca aaccggtgcg ctggacagtt gcgggatgcc
      481 gcgtaccact gcgtggtggc cgtccgtgcg ggcatcggcc cccagccgga ccatctcctc
      541 gacgaaccgg aaccgggcct cgaagacgtt ctcggtgatc atcgacgtgc catcggcgat
      601 cgacgccagc gcgatggcca tcggctgcag atcggtcggg aaccccggga acggcagcgt
      661 cgcgacattg accgccttgg gccgctcgta ctgaaccacc cggaagccgt cgtcggtctg
      721 cgtgacggtg gcgccggcgt cgtgcagctt gtgcagcacc agctgcaggt gctgagggtc
      781 gatgcccgtc accgaaatgt ccccgcgcgt catcgcggcg gcgatgcccc atgtggcggc
      841 gacgatgcgg tcgccgatca cccggtgctc ggtgggatag agccgatcga cgcccgtgat
      901 cgtcatggtc ggcgtccccg ccccggagat ctgggcgccc atttggttga gcatcgtgca
      961 caggtcgacg acgtcgggct cgcgcgctgc gttgtgaatc gtcgtgacgc cctcggcgac
     1021 caccgcagcc atcaggatgt tctccgtcgc gcccaccgac gggaactcca attgaatttc
     1081 cgcgccgcgc aaatgctctg cctcggcgac gacacagccg tgctcgatgt tgcagcgcgc
     1141 acccaactgc cgcagaccgg cttggtgcat atccaacgga cgcgacccga tggcgtcgcc
     1201 gcccggtagc gcgaccttgg cccgcttgca gcgtccgacc aacggtccca gcacacacac
     1261 agatgcccgg aactgacgga ctgcggcgaa atccgcgtcg tacttgggtt catcgggtga
     1321 ggtgacccgc acgacaccac cgtcgagttc gacggtggcg cccagaccgc gcagaacctc
     1381 cgccatcagc ggcacgtcca ggatgtccgg acagttggtg atcgtgctgg ttccctcggc
     1441 caggagcgca gccgccatca gcttcaatac gctgtttttg gcccccccga cagcaacttc
     1501 gcctgataac cggttcccgc cggtcaccac gaatcgctcg ctcacgcggg ttagtgtaaa
     1561 cagcggcacc ggacggtgtc agtttcccag gccactctgc ccggtacggt ttgaccatgg
     1621 cagttcatct gacccgcata tacacccgga ccggcgacga cgggaccacc ggtttgagcg
     1681 atttcagcag ggtctcaaag aatgacgccc gactggccgc gtatgccgac tgcgacgaaa
     1741 ccaatgccgc catcggcgtc gcgatcgcat tgggaaaccc cgatgagcaa attcgcaagg
     1801 tgctacagca gattcagaac gacctcttcg atgcgggcgc tgacctgtcg acgccggtcg
     1861 tagagaatcc cgaacatccg ccgctgcgca tcactcagag ctacatcgat cggttggagt
     1921 catggtgtga cgagttcaac gaaccgctgc cggcactgaa ttcgtttgtg ctgccaggcg
     1981 gtacgccgct ctcggcgctg ctgcacgtgg ctcgcacggt ggcgcgaagg gcggagcgat
     2041 ccgcatggga cgccgtcaac acacataagg actcggtcag cgtgctaccg gctaagtacc
     2101 tcaaccggct gtcagacctg ctgttcattc tgtcgcgagt agccaacccc gatggtgatg
     2161 tgttgtggca acccggcgga tgagcgcttg cgcgaagacc gaaaggccgg gcccggcaat
     2221 cgattgaatc aaaaggttcg gcgccgtgcc cgtggggacg gccgcgactc gagccacgat
     2281 tgaaacgccg ttaacgcacc ccgatccaac gccacttcat atccgcggcg gcgttccggg
     2341 ctggtgtcgc gcagttccag aatgacgatc gcgtcggtca tgatgtcgaa ttcgtcgccc
     2401 cgaggcgacc gtcgcgagac gatctcaagc ccgcggcgac tcagcctgcg gtcgggccac
     2461 cagcgcaggc tggacagccg atagaaccca gcttccccac cgcggtaacg cattacgccg
     2521 tggcgccacc catgcccgcc gaccgcggga acatcccgca agatcgccgc ggtgccgccg
     2581 acttggcgca acttccacag ccgataacta agtgcgaccg aaaagaccgc cagtacaccg
     2641 accagcgcga ccatcaccat catggacgcg ctcatcggcg ggcccgctag tcgatctggc
     2701 ccagggcgcg aagcctcgct cggcccttgg cggcggtagc cggatcgtcg gattcggcgt
     2761 cgcgctgtgc ggcggcttcg tcgatctccg aatcgaattc ggcgctttcg gccaggacgg
     2821 tcacgccgtg ctcagtgacg gacaaaaagc ctccgtcgac ggcgatgcgc agatcgtcct
     2881 caccttcccg ctcgacccgc accatcgcgt cgtcgaccaa ttgcgcgacc aacgggatgt
     2941 ggttgggcag gatgccgatc tcgccggagg tggtgcgggt gaacaggaat gtgccctggc
     3001 ccgaccagat ctcgcgatcg acagcgacga tgtcgatgtt cagttcggcc atctcacatc
     3061 acctttcggt gcgggcaagg cctagtcatc cttcttctcg ccctcgtccg acttgtcgga
     3121 gccattgttc ttgccgccgc cctcaagctc cacaccgaag ctcttggcct tcttcgccag
     3181 atcctccagg ccgccgatca agaagaaggc ctgctctggc acgtggtcga attcaccctt
     3241 ggtgagccgg tcgaacgcct cgatggtctc cttgaccgga acggtcgaac ccggctgacc
     3301 ggtgaactgc tcggccgcca tcatgttctg cgacaggaag cgctcgatgc ggcgggcacg
     3361 ctgcacaagc tgcttgtcct cttcggacag ctcgtcgata ccgagaatcg cgatgatgtc
     3421 ctgaaggtcc ttgtaccgct gcaggattcg gatgacttcc tgagcgacgc ggtagtgctc
     3481 gtcaccaacg accgaggggt cgaggatcgt cgagctggac gccagcgggt cgacggccgg
     3541 gaagatgccc ttcgagaaca ccgcacggct cagctccgtc gtcgcatcca ggtgcgcgaa
     3601 cgtcgtcgcc ggcgcgggat cggtgtagtc gtcggcgggc acgtagaccg cctgcatcga
     3661 cgtgatggag cggccacgcg tcgaggtgat gcgctcctgc agctcgccca tctcgtcggc
     3721 cagcgtcggc tggtaaccca cggcggacgg catacggccg agcagcgttg acacctcgga
     3781 gccggcctgc gtgaaccgga agatgttg
//
LOCUS       DASRJA010000673        14398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2388184,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000673 DASRJA010000000
VERSION     DASRJA010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14398
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2388184"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFC01_30220"
     CDS             complement(<1..674)
                     /locus_tag="VFC01_30220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN83925.1"
                     /translation="MAVKNSPSGMVTLLKPLGSAIEMNLAGAIRTRRRGYRGWTGAVN
                     TDYDPLDPATAAQPHEAYRALHRSGRVHYNPKRATWILSRLNDVRAALRDTDQVTSTR
                     GVTRVRMAADLLVLTDGDEHARLRKQVQPAFTRGALENWHEIADKLAAELVSEVLAEP
                     GCDVVQRLAIPMPMRMIAAILGVPDTDVADFRRWSENSVRIINFSPTPKGVVDIANSM
                     RAVIALR"
     gene            1341..1883
                     /locus_tag="VFC01_30225"
     CDS             1341..1883
                     /locus_tag="VFC01_30225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN83926.1"
                     /translation="MAREDWLVGRDRGSEAADRIYAAASELVSREGFEALTIEALAAE
                     VHCSPATIYRHAGGKAAIREAVTMRTSSRIVGLVRQAIKGLEGTERVVTAILVALEHI
                     RADPLGDLMMGSIRPSHEGEWLTASPGVARLAQEVIGSDDPLAAQWLLRVTLALWYWP
                     AEDRDIEYALVKRFVGPSFS"
     gene            2282..3958
                     /locus_tag="VFC01_30230"
     CDS             2282..3958
                     /locus_tag="VFC01_30230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016934807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN83927.1"
                     /translation="MAFVITQRCCNDASCVSVCPVDCIRPSPDDPQFSTAEMLHIDPK
                     ACVDCGACVPVCPTSAIDYEVDLPEPLKRYIDINAAYFERHPLHADPRRLDERPSRPP
                     KELGTLRVAIVGAGPAACYAADELLARADVEIELLDRLPTPYGLIRGGVAPDHSATKD
                     VGLTFGARFVTDAVGLHLNVEVGKHITHEELMRHHHAVIYAVGAFEERRLDIPGEDLP
                     GSHPASEFVAWYNGHPDYADMTFDLSGQRAVVVGNGNVALDVARILLLDTDELQNTDI
                     ADYALDALRRSNIREVVLLGRRGPLQAAYSSSEFLAFNHLPGVNVVLDERELNLDPTS
                     RAQFEDPALEPALALKVRLAEEFAHRPLDPHNKRAVFRYCVSPAEILGTDRVEGVRIT
                     ANELADIDGAIKAVATDQTEEIEASMVLRSVGFRARQLPGLPFDEHRGIIPNDCGRVI
                     AADGQPIPGVYVAGWIKRGANGTIGTNKHCARETVANIMEDFISGRLRRPDGDRRSLQ
                     QLLEVRQPKLISGDGWHAIDQAERRRGQSSGRPRVKFTDPAEMVEIARKS"
     gene            complement(4226..5122)
                     /locus_tag="VFC01_30235"
     CDS             complement(4226..5122)
                     /locus_tag="VFC01_30235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83928.1"
                     /translation="MPNSQGFHPDLRVIARVLPWGPYTRRTLPVIRALTRLQALRFSK
                     YVDVAGLPSGISVRLHRPQHTDNAGAALLWIHGGGYVIGSAAQDDRLCRRFANRLGVP
                     VVAVDYRLAPKHPYPAALEDCYEAFAWLTRLPGIDPARVAIGGASAGGGLAAALALLA
                     RDRGELVPLFQLLVYPMVDDRSSDRPSIRDRRYRLWSPNSNRLGWTAYLNGADPQVAV
                     PSRHDDLSGLPPAWIGVGTLDPLHDENVEYARRLKNAGVACDLDIVTGAFHGFDIVAP
                     AAGVSRAFFDRQCEALQKAFSS"
     gene            complement(5122..6042)
                     /locus_tag="VFC01_30240"
     CDS             complement(5122..6042)
                     /locus_tag="VFC01_30240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015354420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN83929.1"
                     /translation="MSARKWTEADVPDQTGRVAIVTGANTGLGYDTARVLAERGAMVV
                     LAVRDPEKGKAAQDRILNTAPRADVSIQQLDLGSLRSVRAAAEDLSAAYPRIDLLINN
                     AGVMIPPKQVTPDGFELQFGTNYLGHFALTGLLLKNLISVDGSRIVVVSSNAHKLGGA
                     IHFDDLQWERRYNRGAAYAQSKLANLMFCYELQRRLAAADVPTIAVAAHPGYTDSELI
                     RHVWKPVVPLMRLFGPVVGQDPAKGALPQLLAATDPQVRGGQYWGPRGLFELKGYPKL
                     VTASAKARDEAVQKRLWTVAKELTGVTFPV"
     gene            6784..6927
                     /locus_tag="VFC01_30245"
     CDS             6784..6927
                     /locus_tag="VFC01_30245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83930.1"
                     /translation="MPVQGSYGNAWLIESNVIPAGYFAVVASSGPDSTDNAVARRMHK
                     SPG"
     gene            complement(7397..8701)
                     /locus_tag="VFC01_30250"
     CDS             complement(7397..8701)
                     /locus_tag="VFC01_30250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN83931.1"
                     /translation="MFSETRYAMNGDLRVAYRASPPGPRDIVFVCNPITSCEVFPELP
                     SAQAWFEAMTSLGRVIYFDVPGTGVSDPATWSLELWADSITAVLDDLGSSEAVLVAID
                     GSFVQTALFVAVNPSRTSALVVLEGYADPLAERTQGLDLEETVAAYVAMWGTGEFQHL
                     SNPDMPWNEEIRAAWARHERCAVSPGAVAQMVTQIAELNVRPLLPAIRVPTLVVHHAD
                     DQVIPPEWGKYIADHISGAKYVELPGRNLYHFVEPWRDSFQEIAEFLTGQQADVADDR
                     VLATVLFTDIVGSTRRAAEIGDRDWHALLDAHDAVVRSQLARFRGREVNTSGDGFLAM
                     FDGPQRAIRCAMAIRDAVQALGIEVRAGLHTGECEVRGEDIGGIAVHIGARVSALAGP
                     NDVLVSSTLRDLVIGSGLEFEDRGAHALKGVPGEWHLFAVAS"
     gene            complement(8782..9219)
                     /locus_tag="VFC01_30255"
     CDS             complement(8782..9219)
                     /locus_tag="VFC01_30255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83932.1"
                     /translation="MKSMTLAIATAALTAAASVVAPPAQAMMSPGNYDVNTNRYERAS
                     WVWFISPCQPPDDSCLDISAIPRLKFYAYYDGDAHLVNGQYTLNVDVPDGLQCLPGYA
                     MPTHETWTWDQVSLFGTIESNYDVGCFNGPPGSQFWTFALQRL"
     gene            complement(9273..10637)
                     /locus_tag="VFC01_30260"
     CDS             complement(9273..10637)
                     /locus_tag="VFC01_30260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN83933.1"
                     /translation="MSVDDIRAADNDRKKNRYYFDRHTPEYRLQFEKITEEMQSKCPV
                     AWSETYNGHWVAADSKHVFELARCPAVSNHHDLTGETPYQGITIPKAQRATVVRGGIL
                     EMDEPEHSTYRGALNPYLSPAAVKRWKPFVDEIVRAALDEKIESGRIDFVDDLANIVP
                     AVLTLAMMGIELKKWPVYSEPAHLSVSTPEHSPDAPRVAELNRQMGIDMINTMNEVRE
                     NPRPGLVNALLQLRIDGEPAPDLEILGNLGLIIGGGFDTTTALTAHALEWLSEHPNKR
                     ELLSRERDTLLDPATEEFLRYFTPAPGDGRTFSEDIEVEGQQFKEGERLWVSWAMANR
                     DPAVFDSPNEIILDRKGNRHFSFGIGVHRCVGSNVARTVFKSMLTAVLDRMPDYVCDP
                     EGTEHYETIGVIQGMKHLPATFTPGKRLGPGLDETLEKLQRICDEQELARPITERKES
                     AVID"
     gene            complement(10651..10845)
                     /locus_tag="VFC01_30265"
     CDS             complement(10651..10845)
                     /locus_tag="VFC01_30265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN83934.1"
                     /translation="MKVFVDSARCQGHTLCSMIAPDSFELSDIDGTSSPVNEVVPPDQ
                     EDAVREAVHSCPEQAITIEE"
     gene            complement(10858..11718)
                     /locus_tag="VFC01_30270"
     CDS             complement(10858..11718)
                     /locus_tag="VFC01_30270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZN83935.1"
                     /translation="MGRVQGKVAFVTGAGRGQGRSHAIRLAEEGADIIAVDRCEDFAT
                     IGYPMATPEDLEETAQFVEKTGQRIVTAKVDVSDAGQLKSAVDNGVSELGGLDIVVTS
                     AGIAGMKGSGDMHAWVDVMNTNLIGTLNAIHVALPHLREGASIIATGSTAALMDVSKN
                     DNPGTDPGGAAYLHSKRLLSQYVHNLATELAPRGIRANVIHPTNCNTPMLQSEPMYRS
                     FRPDLEHPTKADAEPVFYVQQAMKVPWIEPEDISNMVLFLASDEARYITGTQQRVDAG
                     GYLKWYDYHI"
     gene            complement(11736..12434)
                     /gene="fabG"
                     /locus_tag="VFC01_30275"
     CDS             complement(11736..12434)
                     /gene="fabG"
                     /locus_tag="VFC01_30275"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZN83936.1"
                     /translation="MKTAVVTGGGSGIGQAVARRLRADGMDVATIDLNPSDEAFSFTA
                     DVTDRDAVEEALAAIRAQLGPVTVLVNAAGLEKFKRFTELKFEDWQRVIDVNLNGVFH
                     CIQAVLPDMIEAGWGRIVNISSSSTHSGQPYMSPYVAAKSAVNGLTKSLALEYGPAGI
                     TVNAVPPGFIDTPMLRKSAERGYLFIDKNIESTPVRRMGRPEDIAAACAFLISEEAGY
                     ITGQILGVNGGRNT"
     gene            12685..14169
                     /locus_tag="VFC01_30280"
     CDS             12685..14169
                     /locus_tag="VFC01_30280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN83937.1"
                     /translation="MQETPTILSDAASAGTAQRRLLIDGKLLDTDRTFPSINPATGEV
                     LGYAPDATVQDAEAAVAAARRAFDTTDWSTNTELRIRCLEQLHRALVDHRDELAALTT
                     AEVGATPALIAGAQLDQPVEIVRYYADLLKNYSMTEDLGNIESRGMQHHRWVEKEAAG
                     VVAAIIAYNYPNQLALAKLAPALAAGCTVVLKGAPDTPLITLAIGELIANHTDIPAGV
                     VNVLSGAAPGVGAVLTTSPDVDMVSFTGSTPTGRRIMAAASDTLKRVFLELGGKSAAI
                     VLDDADFNTAALFSAFSMVTHAGQGCALTSRLLVPRKHKDEIVELIKNNFAMVRYGDP
                     TDPKIYMGPLISEKQRDKVDGMVKRAVEAGATLVTGGEKVDPGFFYTPTLLADVDPDS
                     EIAQEEVFGPVLVVIAYEDDDDAVRIANNSIYGLSGAVFGSQDRALAVARRIRTGTFS
                     INGGNYFSPDSPFGGFKQSGIGREMGIAGLEEFLESKTFATVVS"
     gene            14166..>14398
                     /locus_tag="VFC01_30285"
     CDS             14166..>14398
                     /locus_tag="VFC01_30285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN83938.1"
                     /translation="MRPLEGIRVLEVAMYGFVPSAGAVLGEWGADIIKVEHAVTGDPQ
                     RGLRQTGPLRVEGDPNPNIEHANRGKRSIGLDMS"
BASE COUNT         2646 a         4415 c         4637 g         2700 t
ORIGIN      
        1 cgtagtgcga tgactgcacg cattgaattc gcaatgtcca caacaccttt gggtgtcggt
       61 gagaaattaa tgatccgaac gctgttttct gaccatcgcc ggaagtcggc aacgtcggtg
      121 tctggcacgc ccaggatcgc ggcgatcata cgcatcggca tcggtatggc cagcctttgt
      181 accacgtcgc atccaggttc ggcgagcacc tcggacacca gttcggctgc gagcttgtcg
      241 gcaatttcgt gccagttttc cagcgcacca cgcgtgaagg cgggctggac ttgcttacgt
      301 agccgagcgt gttcgtcacc gtctgtgagc actaagagat cagccgccat tctgactcgc
      361 gtgacccccc gggtgctggt gacctgatct gtgtcgcgta atgcggcgcg gacgtcattc
      421 agtcggctca ggatccaggt ggcgcgcttc gggttgtagt gcacacgccc gctgcgatgc
      481 agcgctcggt aggcctcgtg gggctgagcc gcggtggcgg ggtccagcgg gtcgtagtcg
      541 gtgttaaccg cgccagtcca acctcgatag ccccgccggc gcgtgcggat cgccccggcg
      601 agattcatct ctatggcgct gccaagcggt ttgagtaatg taaccatgcc actcgggctg
      661 ttcttcaccg ccatgaccac tgcacctttc gcgaattgct gaggagcgca aaagtctcgg
      721 gccgaccggc ggcaccgtgt gggtcggcgc aaagagcgtt gtgcgaacga atgagcgtct
      781 cctaagactc gccgacgcgc gagcatgcag gacgggtcgg cggtgcccac caatagtgat
      841 ggcacttcac cgtcatgcca tcaggttcgg cgctggcgca tctgacgtca acgatgcgac
      901 acgaagttga aagtctcatt cggacgccag ccttggaaag ctttcgcatc tgccccatct
      961 gaccagcggc ttcgttagta gaaccccggt ccaaaattct tactgcgatg aagccgtttc
     1021 gtctgatcgc tcaaagaacg gtggccggtg cgcaagggat tgcgctgcgg aatcggaatc
     1081 cccgggcccg cgcacttgcc gctgggagga aaggggctcg acggccctct ctgagctgcg
     1141 cctgaagtcg tcattgcccg gtgtatcgag ttctgatctg tgagcatcgc actcacgcgt
     1201 gcgggtcgaa atcgagaggc caatggcggg gtattctgat catttctcac gagaggcgta
     1261 tgaactgcgc attaatttag tgaagtactc aacagaattc tcaacgcgag agggcgagtt
     1321 cggcgggacg gatgggtgtc atggcgcgtg aggattggct ggtggggcgc gaccggggca
     1381 gcgaagccgc cgaccggatc tacgctgcgg catcagagct ggtctcgcgc gaaggattcg
     1441 aggcattaac catcgaggca ctggctgccg aggtgcactg ttcgccggcc acgatctatc
     1501 gtcacgccgg cggcaaggcc gctatccgtg aagctgtgac gatgcgcacc tcctcacgaa
     1561 tcgtcgggtt agtgcgtcag gcgatcaagg gtttagaggg tacggagcgc gtagtgacgg
     1621 cgatcttggt tgcgttggag cacatccggg ccgacccgct cggtgacctg atgatgggtt
     1681 cgatccggcc gagccacgaa ggcgaatggt tgacagcgtc gccgggcgtt gcccggctgg
     1741 cccaggaagt gatcggctcc gacgaccccc tggccgcgca gtggctgctg cgggtaaccc
     1801 tggcgctgtg gtactggccg gccgaggacc gtgacatcga atacgcgctt gtaaaacgct
     1861 ttgttggccc gtcgtttagc tagagcaagc agccgaaacc ccgcggtttt ccgcgtaggc
     1921 ggcactcggt ggcatctagc tctgtctgaa tccatctgca actgctcttc gcaccacgag
     1981 agaaaatctc acggacttct cagccatcgt tgcaggtgga gtcggtacga gctgtcgggc
     2041 acgcggctta ccaggtgttg aagcgctttc aacttcatgg catatcgttg acgatgtcgc
     2101 ttggcgttca taaggctgag ttgatggtga tggcgcagcg tccaagcaag gctgtcttcg
     2161 gcggtcggtg gcggcagacg atggtcggag tgggcagcta cgccgtgacg attcagccgt
     2221 ggcgatgcgg ttggcagaca gatcaacgag cgttggctcg cccgaagttg gttgtcagat
     2281 tatggctttc gtgatcacgc agaggtgctg caacgatgcg agctgcgtgt cggtgtgccc
     2341 ggtcgactgc atccgcccct ctccggatga cccccaattc agcacggccg agatgctgca
     2401 catcgatccg aaagcctgcg tcgactgcgg cgcatgcgtg cctgtctgcc caaccagcgc
     2461 gatcgactac gaggtcgatc tacctgaacc gttgaagcga tacatagaca tcaacgccgc
     2521 ctatttcgag cggcacccgc tgcacgcaga tcctcggcga ttggacgaga ggccctcacg
     2581 tccacccaag gaactgggga ccctacgggt ggcgatcgtc ggcgccggac cggccgcatg
     2641 ctatgcggcc gacgaactgc tggcccgagc ggatgtcgag atcgagttgc tggacaggct
     2701 gcccacgccg tacgggctca ttcgcggcgg cgtggcaccg gatcactccg cgaccaagga
     2761 tgtgggcctg accttcgggg cgaggttcgt gacagacgct gtgggtttgc acctcaacgt
     2821 cgaagtcggc aagcacatca cgcacgaaga gctgatgcgc caccaccacg ccgtcatcta
     2881 cgcggtgggc gcattcgagg agcgccggct cgacataccc ggtgaagatc tgcccggaag
     2941 ccatcccgca tccgagttcg tcgcctggta caacgggcat cccgattacg ccgacatgac
     3001 attcgatctg tccggacagc gggcggtggt ggttggaaac ggcaacgtcg ccttggatgt
     3061 ggcgcgaatc ttgctcctcg acaccgatga attacagaac accgacatcg cagactatgc
     3121 gctggatgca ttgcggcgca gcaacattcg ggaggtcgtg cttctgggac gacgcgggcc
     3181 actgcaggcg gcctactcat catcggagtt tctcgccttc aaccacctgc cgggggtgaa
     3241 cgtcgtgctc gatgagcgcg agctgaacct cgacccaacc agccgagccc aattcgaaga
     3301 tcccgctctg gaaccggcgc tcgccctaaa ggtgaggctc gccgaggagt tcgcgcaccg
     3361 tccgctggac ccgcacaaca agcgcgccgt attccgctac tgcgtttccc cggccgaaat
     3421 tctcggtacc gaccgggttg agggagttcg aatcactgcc aacgagctcg ccgacattga
     3481 cggcgcaata aaggccgtcg cgaccgacca gaccgaggag atcgaggcat caatggtgct
     3541 gcgctcggta ggcttccgag cccggcaact acccggcctg ccgttcgatg agcatcgcgg
     3601 aatcatcccc aacgattgcg gtcgggtgat cgccgcagac gggcagccga tcccaggcgt
     3661 ctacgtcgcc ggatggatca agcgtggggc taatggcacg atcggtacca acaagcactg
     3721 cgcccgcgag acggtcgcaa acatcatgga ggacttcatc tccggcaggc tccgccgccc
     3781 cgacggcgat cggcgctcgc tgcagcaact gctcgaagtg cgtcagccga agctgatttc
     3841 gggtgacggc tggcacgcca tcgaccaagc cgaacgccgc cgtggtcaat cctccggacg
     3901 tccgcgggtc aagttcaccg acccagccga gatggtcgag atcgccagga agtcttgaca
     3961 gttcgtcgtt cggacgaatg atcgatgtct tgatcaggcc gacgaccgcg cttgctcgag
     4021 tgcctcaatc gaggttgcat gcgtttctcg tcacagggtc tcctgtcgca agagacgaaa
     4081 cacggaccca gtgtcggcgc gaggaggatc agagatgtcg acagcgttgg gaggggcgtt
     4141 gtcaccaagt tccttggctc tccaccctgt tcgtcagctt agggctgggc ctactactcg
     4201 gatcatgaac ggcccctaga aagctctagc ttgagaatgc tttttgcaga gcttcgcact
     4261 gccggtcgaa gaatgcgcgt gataccccgg cggccggtgc gacgatgtcg aatccgtgga
     4321 aagcgccggt gacgatgtca aggtcgcatg cgacacccgc gttcttgagg cggcgcgcat
     4381 actcgacgtt ctcgtcatgc agcggatcga gggtgccgac gccgatccac gcgggcggta
     4441 ggccagagag atcgtcgtgc cgcgagggca cggcgacttg cggatcggcg ccgtttaggt
     4501 aggcggtcca tcccaaccga ttgctgttgg ggctccagag ccgataacgc cgatctctga
     4561 tgctggggcg atcgctggag cgatcatcca ccatcggata caccaggagc tgaaatagcg
     4621 ggaccaattc gccgcggtcg cgagccaaca gcgcaagcgc cgccgccaag ccgccgccgg
     4681 cgcttgcgcc gccgatggcg acccgcgcgg gatcgatgcc gggcaagcga gtcagccacg
     4741 caaacgcttc atagcagtcc tccagggccg ccggataagg gtgttttggc gcgaggcggt
     4801 aatcgacggc gacgacgggg acacctagcc ggttggcgaa gcggcggcac agccggtcgt
     4861 cctgcgctgc cgatccgatg acgtatccgc caccgtgaat ccacagcagc gcggcgcccg
     4921 cgttgtcagt gtgctgaggc cggtgcagcc gcacgctgat tccgctcggt aggcccgcca
     4981 catcgacata tttcgagaaa cgcagtgcct gtaatcgcgt cagcgcgcga ataactggca
     5041 gagtgcggcg agtgtagggg ccccatggca gcacgcgtgc aatcacccgc aagtcggggt
     5101 gaaacccttg gctgttcggc atcagacggg gaaggtgacg ccggtgagtt ccttagctac
     5161 ggtccacagt cgcttctgga cggcctcgtc gcgggctttg gcactggctg tcaccagctt
     5221 cggatagccc ttgagttcga atagtccgcg cggaccccag tactggccgc cgcgcacctg
     5281 cggatcagtg gccgccagta actggggcag tgcccccttc gccggatcct gtcccacaac
     5341 tggaccgaac agcctcatca acggtacgac cggtttccac acatggcgta tcagctcgga
     5401 gtcggtatac ccaggatggg cggccaccgc gatggtgggc acatcggcgg cagccaaccg
     5461 ccgctgcagt tcatagcaga acatcaggtt ggccagcttc gactgagcat aggcggcccc
     5521 gcggttgtac cggcgctccc actggagatc atcgaaatgt attgcaccgc cgagcttgtg
     5581 ggcgttgctg ctaacgacga cgatccgtga accatcgacg gaaatgagat ttttcagcag
     5641 aagcccggtg agcgcgaaat gaccgagata gttggtgccg aactgcaatt cgaagccgtc
     5701 cggtgtgacc tgcttcggcg gaatcatcac tccggcgtta tttatcagca ggtcgatgcg
     5761 tggatatgcg gcggacaagt cctcagccgc ggcgcgcaca gatcgcaggg atccgaggtc
     5821 gagctgctga atagaaacgt cagcgcgagg cgctgtgttc aggatccggt cctgtgcagc
     5881 cttacccttc tctgggtcgc gaacggcgag caccaccatg gctccacgct cggctaggac
     5941 acgagcggtg tcgtagccca ggccggtatt ggcgcccgtg acaatcgcta cccgtccggt
     6001 ctggtcgggc acatccgcct cggtccactt tcttgctgac atgggagatg atcgctctct
     6061 gtcgttgaga ggcaaatatc ctcgtcgttg acgctttccg atgggcagca acggatcgcc
     6121 accgccatgc cttcaggctg ggtggtgggc tatgcgacgt caacaatgtg gcaagaagtt
     6181 gaaaggccgg ctcagacgct ggccggcgcc tctctcgcac tcacaccctt cgaacagcgc
     6241 gttcgcagat taagtagggc cagaagttct cactgcgata agacaatttt gcagaggctc
     6301 ctgctcgccg gggcgccgcg gtgccccgcg cgactaagcg tgactagttt gacgagacgg
     6361 cttgctacga cagtcgccag atcgaacgca accctgcgaa ggtatcggcc agcgagtacc
     6421 gctgatcgga gcgtgggagt gcatgccgga tcccacatcc tcaaaggcgg gtcagggcgc
     6481 gttgaggact taagcgctgg tcaatcacta cctcgtcagt ggatcgccgg acatcgacag
     6541 cggggatata gagtccgccg tgcaccacat aaccgaacac ggctatgggc tcggtggcgg
     6601 gtcgcagttg ctcatcctcg ccaatcccgt tgagtcggaa gccattcagt cctagcgcga
     6661 cgacgaagac caacaacaac ggtccaggtc gcgaagtacg acttcattcc gagtgccgtt
     6721 gtgccgccgt accttacgga ccagacaatc gttgggcaga agccgcccga caacctagcg
     6781 ggtgtgcccg tgcaaggcag ctacggcaac gcctggttga tcgagtcgaa cgtaattccg
     6841 gcagggtatt tcgccgtcgt ggcgtcgagc ggaccggact cgactgacaa cgccgtcgcg
     6901 cgccggatgc acaagtcacc aggatgacaa gggcttcggc atatcccctg gcccgtggca
     6961 gcaatatccg ctggtggaga gcttctttca gcgcgggctg ggtgcgggta cacggcatcg
     7021 cggcgcggcg gtcgtcgtgc agcttgccgc agccgttctg tgcacgccgc cgtcaatcaa
     7081 gctgggaggg gttcgcgatg acctgggttc cgcaggttcc gaatgaccca tcaattaccc
     7141 gcagccgccg tgcgggtggg ttcgtgaccc gtggcggatc agcgatgacg acagcgagtt
     7201 gggtattccg cgaagcaagg acatgcagag cacgaatgcg caacgacgat cggcctgtcg
     7261 cccgcagtga atcctgagcg cagaggcgat cggcacagcg gtcgcacgcc gggtgcggta
     7321 tgtccggcaa tagtcgcgga agcctcgggt tgcgttgtag cccttcagat gatcggtacc
     7381 cgaaatgggt tcacatctac gatgcgacgg cgaataggtg ccattcgccg gggacacctt
     7441 tgagggcgtg tgcgccgcgg tcctcgaact ccagccctga cccgatcacc aggtcgcgca
     7501 gggtgctgga aacgagcacg tcgttcggtc ctgcgagggc gctgacgcgc gccccgatgt
     7561 gcacggcgat gccgccgatg tcctcgccac gaacctcgca ttcgccggtg tgcaacccgg
     7621 cgcgcacctc aatgccgagc gcctgcaccg cgtcgcggat tgccatggcg cagcggatcg
     7681 ctcgctgcgg gccgtcgaac atcgccagaa agccgtctcc ggatgtgttc acctcgcggc
     7741 cccgaaagcg ggcgagctgc gaccgcacca cggcatcatg tgcgtcgagc agcgcatgcc
     7801 agtcacggtc gccgatctcg gcggcccggc gcgtcgagcc cacaatgtcg gtgaacaaca
     7861 cagtggccag aacccgatca tcggccacgt cagcctgctg gccggtgagg aattccgcga
     7921 tctcctgaaa cgaatcgcgc caaggctcga cgaagtgata cagattgcgg cctggcagct
     7981 caacatactt cgcgcccgaa atgtgatcag cgatgtactt gccccactcg ggcgggatca
     8041 cctggtcgtc ggcgtggtgg acgacgaggg taggcacgcg gattgccggg agcagaggtc
     8101 gcacattcaa ctcggcaatc tgagtcacca tttgcgcaac ggcccctgga cttaccgcgc
     8161 aacgttcgtg ccgtgcccag gctgcccgaa tttcctcgtt ccacggcatg tccggatttg
     8221 atagatgctg gaactcaccc gtaccccaca tggcgacata ggcagcgacg gtttcctcta
     8281 agtcaagtcc ttgtgtgcgt tcagcaagcg gatccgcgta gccctcgaga accaccagtg
     8341 cacttgtccg ggacgggttt accgctacga aaagcgccgt ctgcacgaac gagccgtcaa
     8401 tcgcgacgag gaccgcttca ctacttccga gatcgtcgag gaccgcagtg atgctgtcgg
     8461 cccatagctc taggctccac gttgcgggat cggacacccc cgtgccgggt acgtcgaagt
     8521 agatcactcg accaagagaa gtcatcgcct cgaaccaggc ttgggcagac ggcagctcgg
     8581 ggaagacctc acaactcgtg atggggttac agacgaacac gatatcgcga gggcctggcg
     8641 gcgatgcacg ataggcgacg cgcaagtccc cgttcatcgc atagcgcgtc tccgagaaca
     8701 cggcggaagt gtaagccaaa accgcatgca gtttttgatg tcaggcaacg ccgcttggcg
     8761 gcttcgtgcc gccgccgggt tctagagtcg ctgcagcgcg aacgtccaga attgggagcc
     8821 cggtgggccg ttgaagcagc cgacgtcata attcgactcg atggtgccga acagcgagac
     8881 ctggtcccac gtccatgtct catgcgtcgg catcgcgtag ccaggaaggc actgcaatcc
     8941 gtcgggcacg tccacgttca acgtgtattg gccgttgacg aggtgcgcgt ctccgtcgta
     9001 gtaggcatag aacttcagcc gcggtatcgc gctgatgtcc aagcagctgt cgtccggggg
     9061 ttgacagggc gagatgaacc acacccagga agcccgctca taccggttgg tgttcacgtc
     9121 gtaatttcct ggggacatca tggcctgagc gggcggggcg accaccgacg ccgcagcggt
     9181 cagcgcggcg gtcgcaatcg cgagtgtcat cgacttcatc ctcgtccctt cgtcgacccc
     9241 aacccgatgg ctgccaatca aaccactcgg catcaatcga tgacggcgga ttccttacgt
     9301 tcggtgatcg gccgggccag ctcctgctcg tcgcagatgc gttgcagctt ctcgagcgtc
     9361 tcgtcgagtc cgggacccag acgtttccca ggggtgaacg tggcaggcag atgcttcatg
     9421 ccctgaatga cgccgatggt ctcgtaatgc tcggtgccct cggggtcaca tacgtagtcg
     9481 ggcatccggt cgagaaccgc ggtgagcatc gacttgaaca cagtgcgggc gacattggag
     9541 ccgacgcagc ggtgcacgcc gatgccgaag ctgaagtgcc ggttgccctt tcggtcaagg
     9601 atgatttcgt tgggggaatc gaacaccgcc gggtcgcggt tggccatggc ccatgaaacc
     9661 cagagtcgct caccttcttt gaactgctgg ccctcgacct cgatgtcctc ggagaacgtg
     9721 cgcccgtcgc ccggtgccgg cgtgaagtag cgcaggaact cttcggtcgc cgggtcgagc
     9781 agggtgtcgc gctcgcgact gagcagctca cgcttgttgg ggtgttccga cagccactcc
     9841 agggcgtgcg cagtcagcgc tgtggtggtg tcgaaaccgc cgccgatgat caagccgaga
     9901 ttgccgagga tttccaggtc cggtgcgggc tcgccgtcga tccgcagctg cagcagcgca
     9961 ttcaccagcc cggggcgcgg gttctcccga acctcgttca tggtgttgat catgtcgatg
    10021 cccatctgcc ggttgagctc ggcgactcgc ggggcatcag gggagtgctc gggcgtggat
    10081 accgacaggt gagcaggctc gctgtacacc ggccacttct tcagctcgat gcccatcatc
    10141 gccaacgtga gcaccgcggg cacgatgttg gcgaggtcgt caacgaaatc gatgcggccc
    10201 gattcgatct tctcgtcgag cgccgcacgc actatctcgt cgacgaacgg cttccaccgc
    10261 ttcaccgccg ctggggagag gtagggattc agcgcacccc ggtaggtgct gtgctcgggt
    10321 tcgtccatct ccaggatgcc gccgcgtacc acggtggcgc gctgcgcctt cgggatggtg
    10381 atgccctgat acggcgtctc gccggtcagg tcgtgatgat tggaaaccgc tgggcagcgg
    10441 gccaattcga agacatgctt gctgtcggcg gccacccagt gcccgttgta cgtctccgac
    10501 cacgcgacgg ggcacttcga ctgcatttcc tcggtgatct tctcgaattg cagccggtac
    10561 tccggggtgt ggcgatcgaa gtaatagcgg ttcttcttgc gatcgttgtc ggcggcgcgg
    10621 atgtcgtcga cgctcaagat gttgtctccc ttattcttcg atcgtgatgg cctgctcggg
    10681 gcacgagtgc acggcctcgc ggaccgcgtc ctcctgatcg ggtggaacga cctcgttcac
    10741 cggcgatgac gtgccgtcaa tgtcgctgag ctcaaaggaa tctggggcga tcatcgagca
    10801 cagcgtgtgg ccctggcagc gcgcagaatc aacgaaaacc ttcatgcaac actccgatca
    10861 gatgtggtag tcgtaccact tcaagtagcc gccggcgtcc acgcgctgtt gggtgcccgt
    10921 gatgtagcgg gcctcgtctg aggccaggaa caggaccatg ttgctgatgt cctcgggctc
    10981 gatccacggc accttcatcg cctgctggac gtagaacacc ggttcggcgt cggccttggt
    11041 cgggtgctcg agatcggggc ggaacgacct gtacatcggc tcgctctgca gcatcggggt
    11101 gttgcagttg gtcgggtgga tgacgttggc gcggatgccc cgcggtgcga gctcggtcgc
    11161 aaggttgtgg acgtactgcg acagcagccg cttcgaatga aggtaggcag cgccaccggg
    11221 gtcggtgccg gggttgtcgt tcttggagac gtccatcagc gccgcggtcg aaccggtggc
    11281 gatgatcgac gctccctcac gcaggtgcgg cagtgcgacg tggatggcgt tcagcgtgcc
    11341 gatcaggttg gtgttcatca cgtcgaccca ggcgtgcatg tcgccggagc ccttcatgcc
    11401 cgcgattccg gccgacgtga cgacgatgtc gagcccaccc aattcggaga cgccgttgtc
    11461 caccgcggat ttgagctgtc ctgcatcgct gacgtcgacc ttggctgtca cgatgcgctg
    11521 cccggtcttc tcgacgaact gggctgtctc ctcgagatcc tccggggtgg ccatcgggta
    11581 gccgatggtc gcgaagtctt cgcaccggtc gaccgcgatg atgtccgcac cctcctcagc
    11641 cagccgtatc gcgtggctgc ggccttggcc gcgtcctgcg ccggtgacga atgcgacttt
    11701 cccttgaacg cgtcccattc gaagcattcc tttcgttatg tgtttcggcc gccgttgacg
    11761 cccaagatct ggccggtgat gtaaccggct tcctcagaga tcagaaatgc gcacgccgcg
    11821 gcgatgtcct cgggcctgcc catccgacgc accggcgtcg actcgatgtt cttgtcgatg
    11881 aagaggtagc cgcgctcggc ggacttgcgc agcatcgggg tgtcgatgaa gccgggtggc
    11941 acggcgttga cggtgatgcc cgcgggtccg tactcaagag cgagcgattt agtcagcccg
    12001 ttgaccgccg atttggcggc cacgtacggc gacatgtacg gctgtcccga gtgggtgctc
    12061 gacgaggaga tgttgacgat gcggccccag ccggcctcga tcatgtcggg cagcaccgcc
    12121 tggatgcagt ggaagacgcc gttgaggttg acgtcgatga cccgctgcca gtcctcgaac
    12181 ttcagctcgg tgaaccgctt gaacttctcg agtccggcgg cgttcacgag gacggtgacc
    12241 ggacccagct gagcccgaat tgccgccagc gcctcctcaa ccgcgtcacg gtcggtgacg
    12301 tctgcggtga aggagaacgc ttcatcggac gggttcaggt cgatggtggc gacatccatg
    12361 ccgtcggcgc gcagccgtcg agcgacggct tgtccgatgc cggagccgcc acccgtaaca
    12421 accgcggtct tcacgcgcag gcctcagtta tcaagaaccg tccttccgat tatgagaacc
    12481 gtactctcgg agtgtgcgtt atcgcaagac agcagaggag cctcgttcgc actggtgaga
    12541 cgtttggagg tggtttgtct tagcgttagt tgcaggccag cgggagttct gcacatctgt
    12601 ttgagttctc tgtaggcgaa tgtatgattc gcggcggatg agaatgaagt ttccatcaca
    12661 tgtggatgct tgctgaggag gatcgtgcag gaaacgccca ccatcttgag cgacgcagcc
    12721 agtgccggga cggcgcaacg gcgcctcctg atcgacggaa agctgctcga caccgatcgg
    12781 acgtttcctt ctatcaaccc cgccacgggt gaggtcctcg gctatgcgcc cgatgcgacc
    12841 gtccaggatg ccgaggccgc cgtcgccgcc gcgcgacggg ccttcgatac gacggattgg
    12901 tcgaccaata ccgagttgcg catccgctgc ctggaacagc ttcaccgcgc gctggtcgat
    12961 caccgcgacg agctcgcggc actgaccacc gccgaggtcg gcgcgacgcc ggcactgatc
    13021 gccggtgcgc agctggatca gccggtcgaa atcgttcgct actacgcgga tctgctgaag
    13081 aactattcga tgaccgaaga tctcggaaac atcgagagcc gcggcatgca gcaccaccgc
    13141 tgggtggaga aggaggcggc gggcgtcgtc gccgcgatca tcgcctacaa ctatccgaat
    13201 cagttggcgc tggccaagct ggcgccggcc ttggcggcag gctgcacggt cgttctgaag
    13261 ggggcgccgg acaccccgct gatcacgctc gcgatcggtg agctgatcgc caaccacacc
    13321 gacattccgg cgggcgtagt gaatgtgctc agcggcgcgg ctcccggggt cggtgccgtg
    13381 ctgaccacca gcccggacgt cgacatggtc tcgttcaccg gttccacacc gaccggcagg
    13441 cggatcatgg ccgccgccag cgacacgctc aagcgtgtct tcctcgaact gggcggcaag
    13501 tcggcggcga tcgtgctcga cgacgctgac ttcaacaccg ccgcgttgtt ctcggcgttc
    13561 tcgatggtga cccacgccgg ccaggggtgc gcgctgacgt cgcggctact cgtgccccgc
    13621 aagcacaagg acgagatcgt cgagctgatc aagaacaact tcgcgatggt ccgctacggc
    13681 gatccgaccg atcccaagat ctacatgggt ccgctgatca gcgagaaaca gcgcgacaag
    13741 gtcgacggga tggtcaagcg tgccgtcgag gcgggcgcga cgctggtgac cggtggcgaa
    13801 aaggtcgacc ccggcttctt ctatacgccg acgctgctcg ccgacgtcga ccccgacagc
    13861 gagatcgccc aagaagaggt gttcggcccg gtgttggtag tgatcgccta cgaggacgac
    13921 gacgacgcgg tgcgcatcgc gaacaactcc atctacggcc tttctggtgc ggtgttcggc
    13981 agccaggacc gtgcgctggc ggtggcccgt cgcatcagaa ccggaacctt ctcgatcaac
    14041 ggcggcaatt acttcagtcc cgacagcccg ttcggtggtt tcaagcagtc gggcatcggg
    14101 cgcgagatgg ggattgcggg gctcgaggag tttctcgaaa gcaagacgtt cgcgacggtc
    14161 gtctcatgag gccgctggaa ggtatccgcg tcctcgaggt cgccatgtac gggttcgtcc
    14221 cgtcggcggg cgcggtgctg ggcgagtggg gcgccgacat tatcaaggtc gagcacgccg
    14281 tcaccggtga tccgcagcgc ggcctgcgcc agaccggtcc gctgcgcgtc gagggcgacc
    14341 cgaacccgaa catcgagcac gccaaccgcg gcaagcgcag catcgggctg gacatgtc
//
LOCUS       DASRJA010000674         7775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3838876,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000674 DASRJA010000000
VERSION     DASRJA010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3838876"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..940)
                     /locus_tag="VFC01_30290"
     CDS             complement(23..940)
                     /locus_tag="VFC01_30290"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN83939.1"
                     /translation="MRRAKIQAEDGTILRAYLHSPGPGAHPGIVMCPGFGGVKPHIDP
                     YAALFAEAGFAVLNFDNRGWGTSDGEPRQELNVFKHLTDLRDAITFAESQPEFDSEKG
                     FGIWGSSFSGGLAIVTAANDPRIRCVVAQIPNVSGHRNAVKLFTAEQIREIRRRAATD
                     RLARLAGEPPMTVPMFSEDPDELCAFPGEFPEEYREAIESGIWNNETTIRSLENFLEF
                     EPAGWLPYVTPKPLLMILAEHDRCTFTEVQREVYETAPEPKKLLSYDGGHFDAYTTFF
                     EQTAPPARDWFVEHLGAPSNRHVSESVST"
     gene            complement(942..1781)
                     /locus_tag="VFC01_30295"
     CDS             complement(942..1781)
                     /locus_tag="VFC01_30295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016882411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN83940.1"
                     /translation="MSKDDLQADVFIGSALPFEQGAGTFSATTSTLVSGKSDAVLIDA
                     QHIRTDVAALAEFIQRTGKRLTTIYVTHGHADHWYGAGDLLATYPDARVVATAPVVEY
                     INQAAETEAQQWAAMFGERVAKPTAVPEALDGLTIELEGHELRIVEVGQGDIKPSTVL
                     YIPDLEAVVAGDVVYNQIHAMLGLSGPNGWQRWLQSLDAIEKLSAKMVVAGHRKPESS
                     DYEVSRMLDETRSYISDFADAAQQLDNADQLVQQMKTKYPDFGNRWTLHFSAQSWFSR
                     KRA"
     gene            complement(1774..2646)
                     /locus_tag="VFC01_30300"
     CDS             complement(1774..2646)
                     /locus_tag="VFC01_30300"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83941.1"
                     /translation="MTTAQKQTYAEAPTKVVGTADGFDYAYRDVGTGTVPLVLLQHFR
                     GNLDNWDPALIDALASHRRVVAFDNVGVGGTKGTTPNTVEQMAADAVSFLDAMEFDRV
                     DILGFSIGSFVAQELALMRPASVRNLILASAAPRGAAGMHGWAPWVIDAVGTPQLDPA
                     GYLKVFFAESASSQQAGQQALQRMFTKASGFDQPTTWQTRQAQYDAVCAWGIPNHALL
                     QRLSAIEHRVFIANGESDPMILPHFSYLLAGLIPGAQVKIYPDSAHGFLFQHHAEFAS
                     DVEQFLCINGGPDE"
     gene            2780..3595
                     /locus_tag="VFC01_30305"
     CDS             2780..3595
                     /locus_tag="VFC01_30305"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83942.1"
                     /translation="MSLTRIGDGPPPIALPSWVAGTYLLVGWERVALMTTSPAPAVVL
                     VHGAFADASGFGGVIRELQAAGHTVVAPPNPLRSLAFDAAAIAAHAGAIDGPVVLVGH
                     SYGGAVITQASAHLDNVAGLVYLAAFSLDVGESCASVQQPFPPSLLASTSYPTTFDAP
                     GAPQGPDLYINKERFRETFCADAPVDIAETLFATQRPLSLAALTENATAAGWKTTPSW
                     YLVSENDNAIPPGAERFMAERMGATTKSLAGSHTVFIAQPVAVAQFITEALAA"
     gene            3592..4002
                     /locus_tag="VFC01_30310"
     CDS             3592..4002
                     /locus_tag="VFC01_30310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83943.1"
                     /translation="MTTHSTAVDHPSRRLVVALPQTYEEARERYESLVPEVDFARFSA
                     FASWPATVDFAKIYAPHGFMRYYRSDITAALSGSPSSWKTTQYLMGNHTIAERMFRHD
                     PSVMLHARELDELLAGLITLLGGDAPPHLGTEDV"
     gene            3999..4592
                     /locus_tag="VFC01_30315"
     CDS             3999..4592
                     /locus_tag="VFC01_30315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN83944.1"
                     /translation="MKGLLSDKTAVVTGGSSGIGLATAQRFIDEGASVIVTGRRRTEL
                     DAAAAQLGPHAHAVTGDVTVAADLERRSEAAARLGGIDILFANARVARSAPLGSITKE
                     DFQHLFDINVKGVVFTVQTLLPLIREHGSIILNSLNDADSTLDDDQFKAQRSQGIPLG
                     RLATTDEVADAVVLLASGLSSFTTGAALPVDGGYNQI"
     gene            4610..5914
                     /locus_tag="VFC01_30320"
     CDS             4610..5914
                     /locus_tag="VFC01_30320"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HZN83945.1"
                     /translation="MTATEDANKALVIEAFDALFNRRDYSHAQALWSPDYIQHSAHIA
                     PGRTGLFDLVKSLPAELRYEHQLVLANGDHVILHGRFSNHGQPVNWVVADIVRVADGK
                     LAEHWDVIQDEVAETESQSGLPMFGDTFPAAPSPAPPSLLLQAFVEYLRGMPTVVDTA
                     RTRDFNDGHAALEGTSTYPSSLGGVPAVTVEPLVASPERTVVYFHGGGYVSGSPPDRY
                     LPLAGAVALAAQARVHTVDYRLAPETRFPGAFDDCVAAYRWTVGDGGADPETVAVLGD
                     SAGGNLALAVTVAARDENLPLPSCVAVISPFADLTLSGASMELRKLADPYVTRELLES
                     MAAEYLDGADAADPRCSSVFADLHGLPPLLIQVGEDEILFDDATRIRDAAAAGGVDTT
                     FEPWPHGIHVWPVYISAGLPESALAIEHLAAFLKLHARREVS"
     gene            5911..7017
                     /locus_tag="VFC01_30325"
     CDS             5911..7017
                     /locus_tag="VFC01_30325"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HZN83946.1"
                     /translation="MNEIQPLLQPYRMGEFELANRVVMAPLTRCRATNPGLLPTELHV
                     DYYAQRASAGLIITEGTWISRDSVGWHDVPGLFTDAQVRAWAAVTDAVHHRGGLIFVQ
                     LWHTGSSSHPDFFSGTAPLAPSAVNPVVSSPTPSGNKPTVTPRAMTRDDIRTTINDYA
                     VAAANAMRAGFDGVQLQAGFNYLISQFLNPRTNVRTDGYGGSIENRARLLFDVLDAVG
                     ERIDIGRVGVKAGPAWGERGEFVSTSDTLATSEYVVDRLNDYPLAHWLLMGAMADLSG
                     GPLAGLQGDGMFSHFRPRYRGTLISNVGMTRERGNQLIGDGLADLVAFGETFIANPDL
                     PARFAALAPIQRSDRALHYTSGPRGYTDYPPYQP"
     gene            7027..7578
                     /locus_tag="VFC01_30330"
     CDS             7027..7578
                     /locus_tag="VFC01_30330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HZN83947.1"
                     /translation="MDDFTSADQALVVAHHVVETIGDDDLHRPTPCREWDVQALADHL
                     IDTIARLGAAAELKTTVPDGGSIDGRIQQLTQPLLAEWRRRGLADEVVFSGRTLPAHL
                     ALGILSLELLVHGWDFAVALERPFHVSDAHAAHVLGLASQTLNAQSRAAAGFDPPVPV
                     PADASPLVQLIAFTGRNPLQLDR"
BASE COUNT         1385 a         2567 c         2374 g         1449 t
ORIGIN      
        1 acagcggcat gcgtcctcct acttatgtac ttacagattc gctgacgtgc cggttcgacg
       61 gtgcccccag gtgctcgacg aaccagtcgc gagccggggg agcggtctgc tcgaaaaatg
      121 ttgtgtacgc gtcgaagtgc ccaccgtcgt agctgagcag cttcttgggc tcaggggcgg
      181 tttcgtatac ttcccgctga acctcggtga aggtgcatcg atcgtgttcg gcgaggatca
      241 tcagcagcgg cttgggcgtc acatagggca gccagcccgc cggctcgaac tcgaggaaat
      301 tctccaacga gcgaatcgtc gtctcgttgt tccagatccc tgattcgatt gcttcgcggt
      361 actcctcggg aaactccccc gggaatgcgc acagttcgtc ggggtcttcg gaaaacatcg
      421 gcaccgtcat cgggggctct ccggccaggc gagcgagtcg gtcggtcgcg gctcgacggc
      481 ggatctcgcg tatctgctcg gcggtaaaca gcttgaccgc attgcggtga ccactgacgt
      541 tcgggatctg cgccaccacg caccggatcc gcgggtcatt ggcggcggtg acgatcgcga
      601 gtcctcccga gaaactcgat ccccaaatgc caaatccctt ctcggagtcg aattccggtt
      661 gtgattcggc gaaggtgatg gcgtcgcgaa ggtcggtgag gtgtttgaag acgttcagct
      721 cctgccgagg ttcgccgtcg gaggtgcccc agccccgatt gtcgaaattg agcacagcga
      781 aaccagcttc ggcgaacaag gcggcgtacg ggtcgatgtg cggtttcact ccgccgaaac
      841 cggggcacat gacgatcccg ggatgagcgc ccgggccggg tgagtgcagg tatgctcgca
      901 ggatcgtgcc gtcttcggcc tgaattttcg cacgccgcat ctcaagcccg ctttcttgag
      961 aaccatgact gtgccgaaaa gtgcagtgtc cagcggttgc cgaaatcggg gtatttcgtc
     1021 ttcatctgtt gaaccagttg gtcggcattg tcgagctgct gggcggcgtc ggcgaaatca
     1081 ctgatgtacg aacgcgtttc atcgagcatg cgactcacct cgtagtcgga ggactcgggt
     1141 ttgcggtggc cggcgacgac catcttcgcg gagagtttct caatggcgtc caggctttgg
     1201 agccaacgct gccaaccatt gggtccactc aatccgagca tcgcgtggat ctggttgtag
     1261 accacgtcac cggcgacgac tgcctcgaga tcgggaatat agaggaccgt gctgggcttg
     1321 atgtcgccct ggccgacttc gacgattcgc agttcgtgtc cttctagctc gatcgtcaaa
     1381 ccgtcgaggg cctcgggcac cgcagtcggc ttggccaccc gttcgccgaa catcgccgcc
     1441 cactgctgcg cttcggtctc tgccgcctgg ttgatgtact cgacgaccgg ggccgtcgcg
     1501 accacacgcg cgtcggggta ggtggccaac aaatcgcctg cgccgtacca gtggtcggcg
     1561 tggccgtggg tcacatagat ggtcgtcagc cgcttgccgg tgcgctggat gaactctgcg
     1621 agcgcggcca cgtccgtgcg gatgtgctgt gcgtcgatca gtacggcgtc ggacttgccg
     1681 gacacaagag tggatgttgt cgccgagaac gttcccgcac cctgctcgaa gggcagagcc
     1741 gagccgatga acacgtcggc ctgtaggtcg tctttactca tctggacccc cgttaatgca
     1801 gagaaactgt tctacgtcgg aagcgaattc agcatggtgt tgaaatagga acccgtgcgc
     1861 ggagtcggga tagatcttga cctgcgcgcc cggaataagt ccggcgagga ggtaggaaaa
     1921 gtgcggcagg atcataggat cgctctcgcc gttggcgatg aacacgcggt gctcaatggc
     1981 gctgaggcgt tgcagcagtg cgtgattcgg aatgccccag gcgcacacag catcatattg
     2041 ggcttgtcgt gtctgccagg tcgttggctg gtcgaagcca ctggctttgg tgaacatgcg
     2101 ttgcagggct tgctggcccg cctgttgact cgacgctgac tccgcgaaga aaaccttcaa
     2161 gtagccggcc gggtcgagtt gaggcgtgcc gacggcgtcg atcacccatg gtgcccaacc
     2221 gtgcatcccc gcggcgcccc tgggcgccgc agaggccagt atcagattgc gcaccgacgc
     2281 gggtcgcatc agggcgagct cctgcgcaac aaagctgcca atcgagaagc cgaggatgtc
     2341 gactctgtcg aactccatcg cgtctaaaaa cgagacggcg tccgcagcca tctgctcaac
     2401 ggtgttcgga gttgttcctt tcgttccgcc gacaccgacg ttgtcgaatg cgacgacgcg
     2461 gcgatgagat gccagggcat cgatgagcgc agggtcccag ttgtcgaggt tgccgcgaaa
     2521 gtgctgaagc aataccagcg gcacggtgcc ggtgccgacg tcacggtagg cgtagtcgaa
     2581 accgtccgcg gtaccgacaa ccttcgtcgg cgcctctgcg tacgtctgct tttgtgccgt
     2641 tgtcactggc gcctccgggg tcggtacgta tgcggattcg gagaatggct tgacttcaaa
     2701 gctggcattc agcgagatgg cggtccttca cctattcagg tgaggaccgt tgaccgttgc
     2761 gccgcagcct tccccatcga tgtccctcac ccgaataggt gacggaccgc cgccgatcgc
     2821 actgccatct tgggtggcgg gcacgtatct gctcgtcggc tgggaaaggg tcgcattgat
     2881 gacgacgtca cccgcacccg ctgtcgtcct ggttcatggc gccttcgccg atgcatcggg
     2941 cttcggcgga gtgatccgtg agctgcaggc cgcagggcac acggtggttg ccccgccaaa
     3001 cccgttacgc agtctggcct tcgatgcggc cgccattgcc gcgcacgccg gcgcgatcga
     3061 cggcccggtg gtactggtag gtcattccta cggcggtgcc gtcatcaccc aggcctccgc
     3121 gcacctggac aacgtcgccg gcctggtcta cctggcggcg ttcagccttg acgtcggcga
     3181 gagttgtgcc agcgttcaac agccgttccc gccgtcgcta ttggcgagca cttcctaccc
     3241 cacgacattc gacgcacctg gagcaccgca ggggcccgac ctctacatca ataaagaacg
     3301 gttccgcgaa acattctgcg ccgacgcgcc cgtcgacatc gcagagactt tgttcgcgac
     3361 ccagcgaccg ctgtcactgg ccgccctgac cgaaaacgcc accgcggcag ggtggaagac
     3421 gacaccgtcc tggtacctgg tgtccgagaa cgacaacgcc atccctcccg gcgcggagcg
     3481 gttcatggcc gaacgcatgg gcgccaccac caagtcgctg gccggttcgc ataccgtgtt
     3541 catcgcccag cccgtcgcgg tcgcacagtt catcaccgag gccctcgccg catgaccact
     3601 cacagcaccg cggtcgacca tccctcacga cggcttgtcg tggcgttgcc ccagacctac
     3661 gaggaggcgc gcgagcgcta cgaatcgctg gtccccgagg ttgatttcgc ccggttctcg
     3721 gcatttgcct cgtggccggc cacggtcgac tttgcgaaaa tctatgcgcc tcatggcttc
     3781 atgcggtact accgcagtga catcaccgcc gccttgtccg gctcgccgtc gtcgtggaag
     3841 accacgcaat acctgatggg caatcacacg atcgccgagc ggatgttccg gcacgacccg
     3901 tccgtcatgc tgcacgcgcg cgaactcgac gagctcctcg ccgggctgat cacattgttg
     3961 ggcggcgacg cgcctcctca cctcggcacg gaggacgtgt gaagggtcta ctcagtgaca
     4021 agaccgcggt ggttaccggg ggaagcagcg gtatcggatt ggcgacggcg caacgcttca
     4081 tcgatgaggg cgccagcgtc atcgtcaccg gccggcgccg caccgaactc gacgcagcgg
     4141 cggcgcaact cggtccgcac gctcatgccg tgacgggcga cgtcaccgtt gcggccgatc
     4201 tcgagcgacg gtccgaagcg gcggcgcgac ttggtgggat cgacatcctg ttcgccaatg
     4261 cccgcgtcgc ccgcagtgcc ccgctggggt ccatcaccaa ggaagacttc cagcacttgt
     4321 tcgacatcaa tgtcaaaggc gttgtcttca cggtgcagac gctgctccca ctgatcagag
     4381 agcacggctc gatcatcctg aattccctca acgacgcgga ctccactctc gacgacgacc
     4441 aattcaaagc acaacgcagc cagggcatcc cgctgggacg actagccacc accgatgaag
     4501 tcgccgatgc cgtggtgctc ctggcgagcg gtctcagcag cttcaccacc ggggccgcgc
     4561 ttcccgttga cggcggctac aaccagatct gaatctccag aaaggcgaca tgaccgcaac
     4621 cgaagacgcc aacaaggctc tggtgatcga ggcgttcgac gctctgttca accggcgcga
     4681 ttactcccac gcccaagcgc tctggtcacc ggactacatc cagcacagcg cccacatcgc
     4741 acccgggcgc accggtctgt tcgacctcgt caagagcctg cccgcagaat tgcgctacga
     4801 gcatcaactc gtcctcgcga atggcgacca cgtcattctg cacggccggt tctccaatca
     4861 cggccaaccg gtcaactggg tggtggccga catcgtccgc gtcgccgacg gcaagctcgc
     4921 cgaacattgg gacgtcatcc aggatgaggt cgctgaaacc gaatctcaaa gcggcctacc
     4981 catgttcggc gacactttcc ccgcagcgcc gtcaccggca cctccgtcgt tgctgttgca
     5041 ggcgttcgtc gaatacctgc gcggcatgcc aacggtcgtc gatacggcga ggacccgaga
     5101 cttcaacgac gggcatgccg ctctggaagg aacctccact taccccagta gtctgggcgg
     5161 cgtccccgcg gtcaccgtcg aaccactcgt ggcgtcgccc gagcgcaccg tcgtgtactt
     5221 ccacggcggc ggctacgtca gcggttcgcc tccggatcga tacctgccgc tggcgggcgc
     5281 cgtcgcgctc gccgcgcagg ccagagtgca caccgtcgac taccggctgg ctcctgaaac
     5341 tcgtttcccc ggggcgttcg acgattgcgt cgccgcctac cggtggaccg tcggagacgg
     5401 tggagccgat cccgagaccg tcgccgtgct gggggactct gcgggcggca acttggcgct
     5461 ggccgtcacg gtcgccgccc gcgacgagaa cctgccgctg ccttcgtgcg ttgcggtgat
     5521 ctcacccttt gcggacctca cactctctgg ggcttcgatg gaattgcgaa agctcgcgga
     5581 cccctacgtc acccgcgaat tgctggaatc catggcagcc gaatatctcg acggtgccga
     5641 cgcggccgat ccccgctgtt catccgtttt cgcagatctg cacggtctgc cgccgctgct
     5701 gatccaggtg ggcgaagacg agatcctgtt cgacgatgca acgcgcattc gcgacgccgc
     5761 cgcggccggg ggagtcgaca ccacgttcga gccgtggccc catggcatcc acgtctggcc
     5821 cgtctacatc tccgccggac taccggaatc cgcgctcgcg atcgaacacc tggcggcctt
     5881 cctgaaactc cacgcccggc gcgaggtctc gtgaacgaga tccagcccct gctgcagccg
     5941 taccggatgg gtgagttcga gctcgccaac cgtgtcgtca tggcaccgct gacacgttgc
     6001 cgcgcaacga atcccggcct gttgccgacg gaactgcacg tcgattacta cgcccagcgt
     6061 gcctcagccg gattgatcat caccgaaggc acctggatca gtcgcgactc cgtcggctgg
     6121 cacgacgttc caggactgtt caccgacgcc caggtgcgtg cctgggcggc ggtcaccgac
     6181 gccgtgcacc accgaggcgg cctcatcttc gtgcagctct ggcacacggg ctcgtcgtca
     6241 catcccgact tcttctccgg cacagcacca ttggcgccgt cggccgtcaa tcccgtagtg
     6301 agttcaccca cgccatcggg taacaagcca accgtgaccc cgagggccat gacgcgagac
     6361 gacattcgca ccacgatcaa cgactatgcc gtcgcggctg ccaacgcgat gcgcgccgga
     6421 ttcgacgggg tgcagttgca ggcgggattc aactatctga tcagccagtt cctgaacccc
     6481 cgtaccaatg tgcgtaccga cggttacggc ggctcgatcg aaaaccgggc acgtctgctg
     6541 ttcgacgtgc tcgatgctgt cggcgagcgt atcgacatcg gaagggtggg cgtcaaagcc
     6601 ggtccggcgt ggggtgagcg tggtgagttc gtctcgacca gtgacacatt ggccacctcc
     6661 gagtacgtgg tcgaccggct caacgattac ccgttggcgc actggttatt gatgggcgcg
     6721 atggccgatc tcagcggtgg cccgctagcg ggactccagg gcgacggcat gttcagccac
     6781 ttccggcccc gctaccgtgg caccctcatc tccaatgtcg ggatgacccg cgaacgggga
     6841 aaccagctca tcggcgacgg gctggccgat ctcgtcgcat tcggcgaaac gttcatcgcc
     6901 aacccagacc tgcctgcacg ctttgccgcc ctcgcgccga tccaacgatc cgaccgcgcc
     6961 ctgcactaca cgtcgggtcc ccgcggatac accgactatc cgccctacca gccctagcga
     7021 ggacccgtgg acgacttcac cagtgctgac caggcattgg tcgtggcaca ccacgtcgtc
     7081 gaaacgattg gcgacgacga cctgcaccgc cccacgccct gtcgcgagtg ggatgtgcag
     7141 gccctcgccg accatctcat cgacaccatc gcccgcctcg gcgccgccgc tgagctaaag
     7201 accacggtgc cggacggggg ttccatcgac gggcgcatcc agcagttgac gcaaccgctc
     7261 ctggctgaat ggcggcgccg aggattggcc gacgaagttg tgttcagcgg gcggacgctg
     7321 cccgcacatc ttgcgctcgg catcctgtcc ctcgaactat tggtgcacgg ctgggatttc
     7381 gccgtggctc ttgagcgccc gttccacgtc tcggacgccc acgccgctca tgtcctcggt
     7441 ctggcatctc agacactcaa cgcgcaaagc cgcgccgcgg cgggattcga cccacccgtg
     7501 ccggtgcccg ctgacgccag tcctctcgtc caactcatag ccttcactgg ccgcaacccg
     7561 ctgcagctgg accggtaatg acatcggcga atatcaccag ccaagacaag cgcctgtagt
     7621 aaggacgccc gcgtatctga gcagtttgtt tccaagagat aagacagcgt cagaagttgg
     7681 tcgttgcggg acatcgctga gctgggggtt cgctcgtagg tttctcatca cgaactcagc
     7741 gatgagttcc ccaagacaaa aggccaacga ttccg
//
LOCUS       DASRJA010000675         5157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_131685,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000675 DASRJA010000000
VERSION     DASRJA010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131685"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFC01_30335"
     CDS             complement(<1..346)
                     /locus_tag="VFC01_30335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN83948.1"
                     /translation="MTDNAYLAGLRLTGKKVVVVGGGTVAQRRLPLLIANGADVHVIT
                     SSATPAVEGMSGITLTLREFRDGDLDGAWYAIAATDDPDVNAAVVAEADRRRLFCVRA
                     DAAREGSAVTPAS"
     gene            complement(372..770)
                     /locus_tag="VFC01_30340"
     CDS             complement(372..770)
                     /locus_tag="VFC01_30340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZN83949.1"
                     /translation="MVNTLRRAVADTSVLIGVEAVRFDADFLRDYELGVSTVTIGELR
                     LGVLQAGDPAIAARRLSTFQLAQRFEPLAIDEAVAEAWALLVSQLRAASRKAPINDTW
                     IAATAIAHKVPVVAQDADYDHMPNLEVIRI"
     gene            complement(764..1018)
                     /locus_tag="VFC01_30345"
     CDS             complement(764..1018)
                     /locus_tag="VFC01_30345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014983193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HZN83950.1"
                     /translation="MAAIPARDLRNRTAEVLRRVEAGEEIEVLKDNRPVARIVPFRNR
                     RAWVPAAEIAHELARLGPDTTGLAEELRATLTDTTEDLRW"
     gene            complement(1067..2434)
                     /locus_tag="VFC01_30350"
     CDS             complement(1067..2434)
                     /locus_tag="VFC01_30350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HZN83951.1"
                     /translation="MSTPAVVIAAPASGSGKTTVATGLMGALRRVAPFKVGPDFIDPG
                     YHALAAGRPGRNLDPVLVGDQLIGPLYRHGSAGADIAIVEGVMGLFDGRISDEVAGTA
                     QGSTAHVAALLGAPVILVVDARGQSHSIAALLHGFSTFDQSVRIAGVILNRIGSPRHE
                     EVLRQACAHAGVPVFGAIPRADELAVPSRHLGLVTAVEYGEQARAAVDAMTELVGRHV
                     NLDAVAAAATSRVSGEPWDPAVAEPAAENVTVALAAGKAFSFGYTEHGELLRAAGAEV
                     VEFDPLTDPLPPQTAALVLPGGFPEQFGTDLSSNDVVRQQIHELAASGAPIHAECAGL
                     TYLVDDLDGHPMCGVLSGSARFTDQLTLGYREAVAVADSPLHTVGARAMGHEFHRTTV
                     TFADTYPPAWVYAGRDVPTVRDGAIDRGVHASYLHTHPAAHPGAVRRFASRAAQQRVA
                     PAIND"
     gene            complement(2431..3045)
                     /gene="cobO"
                     /locus_tag="VFC01_30355"
     CDS             complement(2431..3045)
                     /gene="cobO"
                     /locus_tag="VFC01_30355"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZN83952.1"
                     /translation="MPQGQPLTIPDDGLSTRARRNAPLLAVHTGAGKGKSTAAFGMAL
                     RAWNQGFDIAVFQFVKSAKWKVGEEAAFRELGRLHDEHGVGGPVEWHKMGAGWSWSRK
                     HGTDVDHAAAAADGWAEIARRLAEQRHDLYVLDEFTYPLKWGWVDVDEVIAVLTSRPG
                     TQHVVITGRDAPQPLLDAADLVTEMTKVKHPMDVGRKGQKGIEW"
     gene            complement(3055..4869)
                     /locus_tag="VFC01_30360"
     CDS             complement(3055..4869)
                     /locus_tag="VFC01_30360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit D family protein"
                     /protein_id="HZN83953.1"
                     /translation="MTAAYPFSAIVGHDRLRLALLLCAVRPEIGGVLIRGEKGTAKST
                     AVRALAQVLAGASLVELPIGATEDRVVGSLDLQKVLRDGEHAFSPGLLARAHDGVLYV
                     DEVNLLHDHLVDVILDAAAMGRVHVERDGVSHSYEARFVLIGTMNPEEGELRPQLLDR
                     FGLTVDVQASRNVDVRADVIRQRMAFEADPSGFAARYAEVDAELARRVAAARARVNSV
                     LLPDNELRRIAALCAAFDVDGMRADLVVARTAVAHAAWRGADEVAEEDIRVAAELALP
                     HRRRRDPFDDPGLDPDLLDEAMAQAGESADEPEPDPDPPSGGESMSEPETSVPQRNSS
                     SAPRPSAAPSPVFRTRAIVVPGVGEGAPGRRSRARNRTGKAIAATEDWQAGHGVHVFG
                     TLLAAAGRQRRPGRPRPEADDVRRAIREGREGNLVIFVVDASGSMAARDRMSAVSGAT
                     LSLLRDAYQRRDKVAVITFRQQAAQLLLPPTSSVHIASRRLARFDTGGKTPLAQGLLA
                     ARDVVVREKARDRARRSLVVVLTDGRATGGPDPLGRARSAAALLVAEGAAALVVDCET
                     SHVRLGLAEQLARYLGAPAVRLAQLRADGLADVVRDAA"
     gene            complement(4866..>5157)
                     /locus_tag="VFC01_30365"
     CDS             complement(4866..>5157)
                     /locus_tag="VFC01_30365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN83954.1"
                     /translation="WLEGPDGEVISTLRLLEEHPGGHKGFRIGRVCTKRSARGHGHTN
                     RLLQAALAEVGDYPCRINAQTYLAEMYTGHGFVRDGEEFLDDGIPHVPMVKP"
BASE COUNT          832 a         1815 c         1714 g          796 t
ORIGIN      
        1 acgacgccgg ggtgaccgcc gagccctccc gggccgcgtc ggcgcgaacg cagaatagcc
       61 gacgccggtc ggcctcggcg accacggccg cgttgacgtc cgggtcgtcg gtggccgcga
      121 tcgcatacca ggctccgtcg aggtcgccgt cgcggaactc cctgagcgtc aacgtgattc
      181 ccgacatgcc ttcgacggcg ggggtggcgc tgctggtgat gacatgcacg tcggcaccgt
      241 tggcgatcaa tagcggcagg cggcgctgtg cgaccgtgcc gccgccgacg acgacgacct
      301 tcttgccggt caggcgaagg ccggcgaggt aggcgttatc ggtcacccgg cgagcttaga
      361 ggttgggcgc atcagatcct gatgacctcg aggttgggca tgtggtcgta gtcggcgtcc
      421 tgagcgacga ccgggacctt gtgggctatg gcggtggcag caatccacgt gtcgttgatc
      481 ggcgctttgc gactcgcggc gcgaagctgg gataccagaa gcgcccatgc ctccgctaca
      541 gcctcatcga tagcaagcgg ttcgaatcgc tgtgcgagct gaaaggtaga caggcgccgc
      601 gccgcgatgg ccgggtcgcc cgcctgtagt acaccaagcc gtagttcgcc gatagtcacc
      661 gtcgaaacgc caagctcgta atccctcagg aaatcggcgt cgaaacggac tgcctcaaca
      721 ccgatcagca ccgaggtgtc ggcgacggcg cgacggagtg tgttcaccaa cgcaagtcct
      781 cggtcgtgtc ggtgagtgtg gcgcgcagtt cctccgcaag ccctgtggtg tctggcccga
      841 gccgggccag ctcatgtgca atttcagccg caggcaccca cgcacgacga ttcctgaacg
      901 ggacgatccg cgcgacgggg cggttgtcct tgagcacctc gatctcctcg ccagcttcga
      961 cgcggcgaag gacctcggcg gttcggtttc gcaggtcacg tgcgggaatg gcagccatgc
     1021 gacaagtgta gcgagccagc tacgcacgta gcaagcaatc gcgcgctcag tcgttgatag
     1081 ccggcgcgac acgctgttga gccgcgcgcg acgcgaacct cctgacagcg cccgggtgtg
     1141 cggccggatg ggtgtgcaga tatgacgcgt gaactccgcg gtcgatggcg ccgtcgcgga
     1201 ccgtgggcac atcacgccct gcgtacaccc acgcaggtgg ataggtgtcg gcgaatgtga
     1261 ctgtggtgcg gtgaaattca tgacccatgg cgcgagcgcc gacggtgtgc agcggcgagt
     1321 cggccaccgc gaccgcctcc cggtagccga gcgtgagctg gtcggtgaac cgtgccgatc
     1381 cggacaacac cccgcacatc ggatgaccat cgaggtcgtc gaccagatat gtcaggcccg
     1441 cgcattcggc gtgtatcggc gcgccgctgg cggccagttc gtggatctgt tggcggacaa
     1501 catcgttgga cgacaggtcg gtgccgaact gttcggggaa gccaccgggc agcaccagcg
     1561 cggcggtctg cggtggcagc gggtcggtca acgggtcgaa ttcgacgacc tcggcgcccg
     1621 cggcgcgcag caattcaccg tgctcggtgt agccgaagga gaacgcctta ccagccgcca
     1681 gcgcgacggt gacgttctcg gcagcgggct cggcgacggc ggggtcccat ggctcgccgc
     1741 tcactcgact ggtggccgcg gcggcgacgg cgtccaggtt tacatggcgg ccgacgagtt
     1801 cggtcatcgc gtcgacggcg gcccgcgcct gctcgccgta ttcgacggcg gtgaccaggc
     1861 cgaggtgtcg tgacggcacg gccagctcgt ccgcgcgcgg gatcgcgccg aacaccggca
     1921 caccggcgtg cgcgcaggcc tgccgcagca cctcctcgtg gcggggcgac ccgatccggt
     1981 tgagaatcac tcctgcaatg cgcaccgatt ggtcgaacgt cgaaaacccg tgcagcagag
     2041 ccgcaatgct gtggctctgg ccgcgagcgt cgacaaccag aatcaccggg gcgccaagga
     2101 gcgcggcgac gtgggcggtg gacccttgag cggtcccggc cacctcgtca ctgatgcgcc
     2161 cgtcgaacag gcccatcacg ccctcgacga tcgcgatgtc cgcgccggcg ctgccatgtc
     2221 ggtatagcgg accgatgagt tgatccccca ccagcaccgg gtccaggttg cgccccggcc
     2281 tgcctgccgc cagcgcgtgg tagccggggt cgatgaagtc cgggccgacc ttgaacggcg
     2341 cgacccgccg cagcgcaccc atcaaacccg ttgcgacggt ggtctttccg ctgcccgacg
     2401 cgggcgcggc aatgacgacc gccggcgtgc tcaccactcg atgcccttct ggcccttgcg
     2461 gccgacgtcc atcgggtgct tgacctttgt catctccgtc accagatcgg cggcgtcgag
     2521 cagcggctgc ggcgcgtcac gaccggtgat cacgacatgc tgggtacctg gccgtgacgt
     2581 cagtacagcg atgacttcgt cgacatccac ccagccccat ttcagcggat aggtgaactc
     2641 gtcgaggacg tagaggtcgt gccgctgctc agccaatcgg cgcgcgatct cagcccagcc
     2701 gtccgcggcg gccgccgcgt ggtccacgtc agtgccgtgc ttgcgcgacc acgaccagcc
     2761 cgcgcccatc ttgtgccact ccacaggacc gccgaccccg tgctcgtcgt gcaatcggcc
     2821 aagttcacga aacgcggctt cctcgcccac tttccacttg gcgctcttga caaactgaaa
     2881 gacggcgatg tcaaacccct ggttccaggc ccgcagcgcc atgccaaacg ccgcggtcga
     2941 tttgcccttg cccgcgccgg tgtgcacggc gagcagcggg gcgttacgcc gggcgcgggt
     3001 cgacaggcca tcgtcgggaa tagtcagcgg ttgaccctgt ggcatcgaaa cctcctaggc
     3061 ggcgtcgcgc acgacgtcgg ccaacccgtc ggcgcgcagt tgtgccaacc gcaccgcagg
     3121 cgcgccgagg tagcgggcca gctgctcggc caaacccagt cgtacgtgcg aagtttcgca
     3181 gtccaccacg agggcggcag cgccctcggc aaccagcagt gccgcggcgg accgcgcgcg
     3241 tcccagcggg tcgggcccac ccgtggcgcg cccatcggtg agcaccacga ccaggctgcg
     3301 ccgcgcccgg tcgcgcgcct tctcgcgcac caccacatcg cgggcggcca ataagccttg
     3361 cgccagtgga gttttcccgc cggtgtcgaa ccgggccagc cgtcggctcg cgatgtgcac
     3421 cgacgacgtc ggcggcaaca acagctgcgc agcctgctgc cggaacgtga tcaccgccac
     3481 cttgtcccgg cgctggtatg cgtcccgcaa cagcgacagc gtcgcaccgc tgaccgctga
     3541 catccgatcc cgggcggcca tcgacccgga ggcgtcgacg acgaagatga ccagattgcc
     3601 ttcgcgccct tccctgatcg cccgacgcac gtcgtccgct tcaggacgcg gcctgccggg
     3661 gcggcgctgg cgaccggcgg cggccagcag cgtcccgaac acgtgcacgc cgtggcccgc
     3721 ctgccagtct tcggtcgcgg caatcgcctt acccgtgcga ttgcgggcac gcgaccggcg
     3781 tcccggcgcc ccttcgccga cgccgggcac cacgatcgcc cgtgttcgaa acaccggcga
     3841 cggcgctgcg ctcggccggg gagcagagct cgaattacgt tgcgggaccg atgtttcagg
     3901 ttcagacatc gattcgccac cactcggcgg atcgggatcc ggctctggct cgtcggcgga
     3961 ttcccccgcc tgagccatcg cctcgtcgag caggtccggg tccagtccgg ggtcgtcgaa
     4021 cgggtcacgc cgacgtcggt gcggcaatgc gagttccgcg gccacccgga tgtcctcttc
     4081 cgctacctca tctgcgccac gccacgcggc atgcgcgaca gcggtgcgcg ccaccaccag
     4141 atcggcccgc atcccgtcga cgtcgaaggc cgcgcacagc gcggcgatgc gtcgcaattc
     4201 gttgtccggc aacagaacag agttgacacg tgcgcgcgcg gcagcgacac ggcgcgccag
     4261 ctcggcgtcc acctcggcat atcgagcggc gaacccggac ggatcggctt caaacgccat
     4321 ccgctgccgg atcacgtcgg cgcggacgtc gacgttgcgg gaggcctgca cgtcgacggt
     4381 cagcccgaac cggtcgagaa gctgcggccg cagttcgccc tcttcgggat tcatcgtgcc
     4441 gataagcacg aatcgcgcct cgtaactgtg cgagacgccg tcgcgctcga cgtgcacacg
     4501 gcccatcgcg gcggcgtcca ggatcacgtc gacgaggtga tcgtgcagca gattgacctc
     4561 gtcgacgtac aacacgccgt cgtgtgcgcg ggccagcagg cccggcgaga atgcatgctc
     4621 gccgtcgcgc agcaccttct gcaggtccag cgacccgacc acccgatcct cggtcgcacc
     4681 gataggcagt tcgacaagcg acgcgccggc caacacctgc gccagcgccc gtacggcggt
     4741 tgacttcgcg gtgcccttct ctccgcggat cagcacgccg ccgatctcgg gccgcaccgc
     4801 gcacaagagc aacgccagcc gcaaccggtc gtgtccgacg atcgcgctga acggataggc
     4861 cgccgtcacg gcttcaccat cggcacgtgc gggatgccat cgtcgaggaa ctcctcgcca
     4921 tcgcgaacga atccgtgccc ggtgtacatc tccgccaaat acgtctgtgc gttgatgcgg
     4981 cacggatagt cgccgacctc ggccagcgcg gcctgcagca gccggttggt atgcccgtgg
     5041 ccgcgggcgc tgcgcttggt gcagacccga ccgatgcgga accccttgtg cccgcccgga
     5101 tgttcctcca gcagccgcag cgtcgagatg acttcaccgt cggggccttc caaccag
//
LOCUS       DASRJA010000676         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1542542,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000676 DASRJA010000000
VERSION     DASRJA010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542542"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="VFC01_30370"
     CDS             <1..171
                     /locus_tag="VFC01_30370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZN83955.1"
                     /translation="FATALMNKDIGLAMDAVRSTGSSAPLGSHAAEIYAKFAADHADK
                     DFSAVIEMIRKG"
     gene            complement(260..1600)
                     /locus_tag="VFC01_30375"
     CDS             complement(260..1600)
                     /locus_tag="VFC01_30375"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HZN83956.1"
                     /translation="MFDSEFSTADDATVVAAIEEATRAEAAAGARRLAAIAEVARRRV
                     VDDDERANWAFDGWASAAAEVAAAMTVGHRRASAQMRIAVALRDHLPRVAALYMQGML
                     SSRVVSAITWRTQLVEDADALALIDAALAEGATRWGPLSEDRLDQAIDAWILRYDPAA
                     VRRSESSTRTRDFSIGDLDDPAETTSVWGRLLATDAAVLKRRVAQMVQSVCDDDPRTI
                     GERRSDAVGAIAAGNSRLACQCGSETCPAGDAPSSSNVVIRVLAEQSAVNAATAGNDA
                     SQEQTSEAATVAPPAALIVGEGVVPTPLLSQLIRGGAKVRPITMPSEEPEPHYRPSAE
                     LAEFVRMRDLYCRAPGCDVPADRCDIDHTIPYPVGPTHASNLKCLCRTNHLMKTFGGW
                     HDIQLPDGTVIWISPSGRKYITKPGSRLFFETWDTTTADLPPRRSWCRTPATAA"
     gene            1756..2571
                     /locus_tag="VFC01_30380"
     CDS             1756..2571
                     /locus_tag="VFC01_30380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN83957.1"
                     /translation="MTFPVRVAVQIQPADTPDYATWRQAVLHAEEIGVDVIFGYDHFH
                     APFIESIADAKPVLAEVQPDVNNFEGWTALASWGEITTRAELGLLVAGIGYRNPDLLA
                     DMARTVDHISGGRAILGVGAGWYEKDYTTYGFDYGTIKSRADHFDEGLLRIERRFDLL
                     QPAPLRKIPILIGGSGPKRTLPAVARHADIWHTFLPIDSYREASARVAELAAGFGRAD
                     SDIERSTLWTDPQSADAYLAAGATLFHTEVRASEGKYDLSTVEKLVDWRDTKR"
BASE COUNT          471 a          887 c          856 g          468 t
ORIGIN      
        1 ttcgcgaccg cactcatgaa caaggacatc ggcctggcca tggatgcggt caggtcgacc
       61 ggttcgtcgg cgccgctggg cagccacgcc gccgagatct acgccaagtt cgccgccgat
      121 cacgccgaca aggacttcag cgcggtcatc gagatgatcc gcaagggtta gaacggtgcg
      181 ggcgcttcgt tgcgcgcgcg ttcggccttg atccgtgcgg cgtattcggc agcgcgggta
      241 cgtcgtcgtc tgggcatcat caggccgcgg tcgccggtgt tcggcaccac gatcgacggg
      301 ggggaaggtc ggcggtcgtg gtgtcccagg tctcgaaaaa cagccggcta cccggtttgg
      361 tgatgtactt cctgccgctg ggcgagatcc aaatgaccgt gccatcaggc aactgaatgt
      421 cgtgccatcc gccgaatgtc ttcatcaaat ggttagttcg acacagacac ttcaggtttg
      481 acgcgtgggt cggcccgacc ggatagggga tggtgtggtc gatgtcgcaa cgatcggccg
      541 gcacgtcgca gcccggcgcg cggcagtaga gatctcgcat tcgcacgaac tcggccaact
      601 ctgccgacgg tcgataatgc ggttccggct cttcgctggg catggtgatc ggacggacct
      661 tggcgccgcc gcgaatcagc tgcgacagca acggtgtcgg cacgacgccc tctccgacga
      721 tcagcgctgc gggcggtgcg accgtagccg cctcggacgt ctgttcctgt gatgcgtcat
      781 tgcctgcagt tgccgcgttg accgctgatt gttcggcaag cacccggatc accacgttag
      841 aggaactcgg tgcatcgcct gcggggcaag tttccgaacc gcattggcac gcgagacggg
      901 aattgcctgc tgcaatagcg cccaccgcgt ccgagcggcg ctcgccgatg gtgcggggat
      961 cgtcgtcaca gacgctctgc accatctgtg cgacacgacg cttcagcacc gccgcgtcgg
     1021 tagccaatag ccgaccccac accgatgtgg tttcggcggg atcatcgaga tcaccgatcg
     1081 agaagtccct agtacgcgtg gatgattcag agcggcgcac cgccgcgggg tcgtatcgca
     1141 ggatccacgc gtcgatcgcc tgatcgagcc ggtcctccga gagcggaccc catctggtcg
     1201 ctccctccgc aagggcggcg tcgatgagag caagcgcgtc ggcgtcttcc acgagttgtg
     1261 tgcgccacgt gatcgcggag accacccgcg agctgagcat gccctgcata tacagcgccg
     1321 caacgcgggg aagatggtca cgcagcgcga ccgcgatccg catctgcgcc gaggcccggc
     1381 gatgtcccac cgtcatggcc gccgccactt cggcggccgc tgaagcccag ccgtcgaacg
     1441 cccagttcgc gcgctcgtca tcgtcaacga ctcggcgccg ggcgacctca gcgatggcag
     1501 ccagccgacg agccccggca gcagcttctg ctcgcgtggc ctcctcgatg gcggcgacga
     1561 ccgtcgcatc atccgccgtc gagaactccg agtcgaacac acttgcgatt atgccaatgg
     1621 ccgccgaccg ccgagaaagt tatccacagt cacggattag tccacagatg cagtccctca
     1681 acacgtttgg gcttgacaac gggtctggtc gtggcggccc ggaataaatc gcggcgagca
     1741 ttgcttgtcg ggtgaatgac ctttcccgta cgcgttgccg ttcaaattca gcccgccgac
     1801 acccctgact acgctacctg gcggcaggct gtcctgcatg ccgaggagat cggggtggac
     1861 gtcatcttcg gctatgacca cttccatgcg ccgttcatcg agtcgatcgc cgacgcgaag
     1921 ccggtgctgg ccgaggtgca gcccgacgtg aacaacttcg agggttggac cgcgctggca
     1981 tcctggggcg agatcaccac acgcgccgaa cttggcctgc tggtggcggg gatcggctac
     2041 cgcaatcccg atctactcgc ggacatggcg cgcaccgtcg accacatcag cggcggccgg
     2101 gccatcctcg gcgtcggcgc tggctggtac gagaaggatt acaccactta cggtttcgac
     2161 tatggaacga tcaaatcgcg ggcggatcat ttcgatgagg ggttgctgcg catcgagcgc
     2221 cggttcgacc tgttgcagcc ggcaccgctg cgcaagatac cgatcctgat cggtggatcc
     2281 ggccccaagc gcaccttgcc cgccgtcgca cgccatgccg acatctggca caccttcctg
     2341 cccatcgact cctaccgcga ggccagtgcc cgcgtcgccg aattggccgc cggattcggg
     2401 cgcgccgaca gcgatatcga gcgctcgacg ctgtggacgg atccgcagtc ggcggacgcc
     2461 tacctggccg ccggcgccac gttgttccat accgaggtcc gcgcgtcgga gggcaaatac
     2521 gacctcagca cggtggagaa gctggtcgac tggcgcgaca cgaagcgctg agcgcccgct
     2581 tcagcgttgc tcgcgaaccc ctagcacgtc gcggaacagc gcctgccgaa gcgccagttc
     2641 ccgtggatca ctgaacgggt agagacccat ccggttcatc ac
//
LOCUS       DASRJA010000677        14929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_740050,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000677 DASRJA010000000
VERSION     DASRJA010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14929
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740050"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFC01_30385"
     CDS             complement(<1..232)
                     /locus_tag="VFC01_30385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZN83958.1"
                     /translation="MTVTSEISAVVAELRREVCDLHAQLTKYQLVIWTAGNVSARVSG
                     HDLMVIKPSGMDYDALTPDDMAVCDLYGNLVDG"
     gene            complement(229..1896)
                     /gene="araB"
                     /locus_tag="VFC01_30390"
     CDS             complement(229..1896)
                     /gene="araB"
                     /locus_tag="VFC01_30390"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HZN83959.1"
                     /translation="MHRQKYTIGIDFGTLSGRAGVVRVDDGEELASAEHIYEHGVLTQ
                     SLPVGGVRLPAQWALQVPEDYRRVLKIAVPEAISRAGIDPPDVIGIGTDFTACTMIPV
                     LADGTPLCETAAFADRPHAYAKLWKHHSPQPQADRINALAAERNEPWLKRYGGFISSE
                     WEFAKALQILEEDPEVYDAIDRWVEAADWIVWQLCGSYVRNACSAGYKGIRQDGRYPT
                     RDFLTALHPKFADFVDAKLDHPIGALGDAAGVLTEQAAQWMGLPAGLPVAVGNVDAHV
                     TSAAANSLEPGQLLAIMGTSTCHVMNSDVLQDVPGMCGVVDGGIVAGYWGYEAGQSGV
                     GDIFAWFVDNCVPGSIHEQARDRNVSVHDLLTEQAAAQRVGQHGLVALDWHSGNRSVL
                     VDHQLSGVIVGQTLATTCADLYRALLEATAYGTRMIVETFVNNGVPVTEVVVGGGLLK
                     NRLLMQIYADVVGLPLSTVNSAQAPALGAAIHAAAAAGAYQDVPAAAKQMGGRTRNAY
                     LPIVDNVARYDRLYAEYVKLHDWFGRGNAMMRTLREISTPATAGASR"
     gene            complement(1905..2924)
                     /locus_tag="VFC01_30395"
     CDS             complement(1905..2924)
                     /locus_tag="VFC01_30395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZN83960.1"
                     /translation="MTAEPIPRRPVMADVARLAGVSHQTVSRVINGSASIRPSTRERV
                     ERAIADLGYHPNTAARALVTRRSKIIGIIGSNTALYGPASIQFSIQDAARAAGYFSSL
                     VTLAAVTHDELRGALNYLSRQSVEAIVMIVTQQEALAVVRSEATTIPLIVVEGELSGR
                     GLSVGVDQMEGARLATQHLIDLGHRDIVHVSGPLTWTEARARRAGYVEVMRAARLPVR
                     ADLEGDWSPARGYAIGRELVKRNDFTAVFVANDQMAIGILHAFAQGGIAVPDDVSLIG
                     FDDVPEAAYLNPALSTVRQDFHAVGQRAIELLTAVLDGVSPATPLLAPELVVRDSTRP
                     HTRTK"
     gene            complement(2921..3919)
                     /gene="yjfF"
                     /locus_tag="VFC01_30400"
     CDS             complement(2921..3919)
                     /gene="yjfF"
                     /locus_tag="VFC01_30400"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HZN83961.1"
                     /translation="MSTPTLRRHPIAPAVAERIRGRYLSPIASVILFVALFTTVIVRY
                     DFASPAQVFLNLLVDNAHLIVLAVGMTFVILTGGIDLSVGSVVALSTVILAKTLQSGW
                     PAPIAIATVLIVGPLLGLLMGTVIEFFELQPFIVTLAGMFLARGLCYVISVNSLPIKD
                     PVLRALGLNYVYLYEDKFIRWTVVIALIVVVAAVFVLHETRFGRTVYAVGGSRQSAQL
                     MGLAVTRTQISVYVISGFCAALAGLLLGVQKLSGYSLNGVGLELDAIAAAVIGGVLLS
                     GGVGFVLGSLIGVLVLGIIQVFVTAENLDSYWTRIMTGILLLVFVLVQRVLVRNTR"
     gene            complement(3916..4989)
                     /locus_tag="VFC01_30405"
     CDS             complement(3916..4989)
                     /locus_tag="VFC01_30405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN83962.1"
                     /translation="MMRRLTASPLLWPTLALIALLGLNVVLTPAFLTVRIEDGHLYGS
                     LIDILRNGAPTMLVALGMTLVIASRGIDLSVGAVVAISGALACAHIASAADPGSVATI
                     VTAMSIALGVAVVLGLWNGMLVSIFGIQPIIATLVLMTAGRGIALLITEGQIVTVTSE
                     PFKVLGAGYALGLPVAILTSLTVFAVVGLITRRSALGMLLESVGVNPAASRLAGVRYR
                     TIVFAVYVFSALCAGIAGLMIASNISAADANNAGLWIEMDAILAVVIGGTSLLGGRFS
                     LTGTILGALIIQTLTTTVYTAGITPETTLVFKAVVVIAVCLLQAPRFRRLLRRRRRAA
                     LQPAVPEPEVAEPAQRADKVAAS"
     gene            complement(4989..6521)
                     /locus_tag="VFC01_30410"
     CDS             complement(4989..6521)
                     /locus_tag="VFC01_30410"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZN83963.1"
                     /translation="MAPTSTAEPQAPVVEMCDISISFPGVKALDGVSFRLLPGEVHAL
                     MGENGAGKSTLIKALTGVYDIDTGTITVAGVPSRFTGPRQAQDAGISTVYQEVNLCTN
                     LTVAENILLGREPRRLGRIDHRALNRRAHELLSQLELDIDPRSTLGAHPIAIQQLVAI
                     ARATAVSARVLILDEPTSSLDAEEVAELFRVIRQLRDGGTAVLFVSHFLDQVYEISDR
                     MTVLRNGRRVGEYLTADLDRHALIAAMLGRELDVLEEIADAEPGAHRDDTVPPIVRAV
                     DVCRRPRVDPITLDIFPGEVVGLAGLLGSGRTEFARLLFGADKATSGQIAVDGKPARL
                     RSPRAAIAHKFAFTSENRKGEGIVGELSVRDNLVLALQARRGFARPLSAKAKSELVHR
                     YMEALDVRPRDPDVLIKNLSGGNQQKVLLARWLITRPTLLILDEPTRGIDIGAKAQIQ
                     KLVTDLATDGMAVLFISAELDEVARISDRVAVMRDGHNIAQIDGGCAVSELTRLIASG
                     SH"
     gene            complement(6616..7596)
                     /locus_tag="VFC01_30415"
     CDS             complement(6616..7596)
                     /locus_tag="VFC01_30415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN83964.1"
                     /translation="MKKILATSLGALIALAMVGCGSGPAPGGGGGSKDGQITMGFSQV
                     GAESGWRTANTKNIQESAAAAGIDLKFSDANGKQENQISAIRSFIQQHVDVIAFSPVV
                     RTGWDAVLLEAKNAGIPVILTDRAVDTQETDVYKTFVGADFVAEGRSAGDWVVKQYAT
                     APGPVNIVELEGTTGADPAIDRSKGFSEAIAANPNLKVIASQTGDFTRSGGKQVMEAF
                     LKANPKIDVVFAQNDDMGLGAIEAIEAAGLKPGQDVKIVAVDATHDGMQALADGKFNF
                     VVECNPLLGPQLMDLAKKVVAGETVAPRVVVPDKTFDQQQAVAALPDRKY"
     gene            7932..9023
                     /gene="chvE"
                     /locus_tag="VFC01_30420"
     CDS             7932..9023
                     /gene="chvE"
                     /locus_tag="VFC01_30420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HZN83965.1"
                     /translation="MRKLIWLAALLAMLTTLALSGCGRSTDTAAGGNGDKGTIGIAMP
                     TKSSERWVADGKNMVDQFQSLGYMTDLQYGDDVVQNQVSQIENMITKGVKLLVVAPID
                     GSSLTTTLQHAADAKIPVISYDRLIKGTPNVDYYATFDNFKVGVLQGSYIADKLGLAQ
                     GKGPFNIELFAGSPDDNNATYFFDGAMSVLKPYIDSGKLVVKSGQTSFDQIATLRWDA
                     GLAQSRMDNLLSQAYTTARVDAVLSPYDGISRGVLSALKSAGYGNAAKPLPIVTGQDA
                     ELASVKSIVAGEQTQTVFKDTRELAKAAVQEASAVLTGGKPEVNDTNTYDNGIKVVPS
                     YLLQPVSVDKSNYQSVLIGSGYYTEAQVQ"
     gene            9041..10549
                     /gene="mmsA"
                     /locus_tag="VFC01_30425"
     CDS             9041..10549
                     /gene="mmsA"
                     /locus_tag="VFC01_30425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZN83966.1"
                     /translation="MRGITKEFPGVTALSEVDLTVKAGAIHAICGENGAGKSTLMKVL
                     SGVYPHGDYSGDILFDGHACEFKDIRASERAGIAIIHQELALVPYLSIAENVFLGNEQ
                     SRFGVIGWDQTLIEAQALLDRVGLRESPQTRVADIGVGKQQLVEIAKALSKKVRLLIL
                     DEPTAALNDSDSRHLLDLIVDLREHGITSIIISHKLNEVMHVADTITILRDGRTIETI
                     AVNDDLAEDRIVRGMVGRDLTHRFPPRADREIGDIAFSVSNWTVLHPIDQHRKVVDDV
                     SLNVRAGEIVGIAGLMGAGRTELAMSVFGRTYGKYVSGKVFKGDREISTRTVSDAIRN
                     GVAYATEDRKHLGLNLMDSIAQSITLPSLRKISRRGVVNQHEEAGVGERFRASMRIKA
                     SSVSAVTGKLSGGNQQKVVLSKWLFTDPDVLILDEPTRGIDVGAKYEIYTIINELAAR
                     GKAVLVISSELPELLGICDRIYTLSQGRLTGEVAQADATQETLMRHMMKGRA"
     gene            10550..11800
                     /gene="mmsB"
                     /locus_tag="VFC01_30430"
     CDS             10550..11800
                     /gene="mmsB"
                     /locus_tag="VFC01_30430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HZN83967.1"
                     /translation="MTTTVEQSSQKPEAGQQPGEASPTPPGLRERVSSFLMSHVRQSG
                     MVVALAAIVLLFQVWTGGILLKPLNVTNIVQQNGYILVLAIGMVVVIISGHIDLSVGS
                     IAGFIGAMSGVLMVRNDMPWLLAVGLCVGIGALIGAWQGFWIAYVGIPSFIVTLGGML
                     VFRGATQYLLEGQSIAPMPHGLEQVSSGFLPEMGTNAVYHWPTVILGAVIMVAAVVVQ
                     IRRRRSQLRYGQEVSATPLFVAKCAAIVAALAVFTLLLASYRGVPIVGIILVVLTLIY
                     AFVMRNTVLGRQVYAVGGNSAAARLSGIRNQRVTLLVFVNMGVLAAIAGLLFAARLNS
                     ATPQAGINFELEAIAAAFIGGASSSGGVGTVFGAIVGGLVLGVLNNGMSIMGIGSDVQ
                     QVIKGLVLLAAVGFDVYNKRKGGR"
     gene            complement(11797..12825)
                     /locus_tag="VFC01_30435"
     CDS             complement(11797..12825)
                     /locus_tag="VFC01_30435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZN83968.1"
                     /translation="MGERVERRPANMFDVAKAAGVSHQTVSRVLNDSASVKESTRSRV
                     NAAIDELGYHRNLAARALATHRSKTLGVICFDSTLYGPASMVYAIEHAARSAGYFVSV
                     ASESTIDSTTLPRALNRLAEQSVEGVVVIAPLSETRHALSRLRGEMPVVAVDGTGGLG
                     LPMVSVDQFDGARQVTRHLLEQGASTVWHVTGAEGWTETDARLAGWRQELAAAGRTIP
                     EPLHGNWSAASGYQAGIALAGRADVEAVFVANDQMALGLLRAFAEAGRAVPEDVLVAG
                     FDDVPEAEFYSPPLTTVRQDFATVGRSSVEMLIARIEDSDSGPIDDQPTLVPAQLVIR
                     QSTQRQSA"
     gene            12917..13909
                     /locus_tag="VFC01_30440"
     CDS             12917..13909
                     /locus_tag="VFC01_30440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZN83969.1"
                     /translation="MQEVMTVARLHGVRDIRTHTESVPAAAAGEELVRVTAVGLCGSD
                     LHWFTEAGIGDARLVRPVVPGHEFAGVIEGGPRHGQHVAVDPALPCRRCEMCLGGYRN
                     LCPDVVFAGHGGQDGGLQQYLVWPSWALHPLPDGLSGVDGAMLEPLGVALHALDLGHL
                     RIGSTVAVVGAGPIGLLLMQAAKGAGAVRVVVVEPLAHRRDAAVRLGADAVLSVGEAA
                     DDVGADAVFELAGTDAAVEIALRAARPGARVVLGGIPDGDRTSFQASLARRKGLTLVL
                     VRRMNEAYPRAIRLAAQGLIDVSSIVTQRYPLERAAEAFAVAADRQGLKVVIEP"
     gene            complement(13896..14852)
                     /locus_tag="VFC01_30445"
     CDS             complement(13896..14852)
                     /locus_tag="VFC01_30445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HZN83970.1"
                     /translation="MQFRPWPVLGEQYELTLEAAGRSVRAVITEVAAALRHLSVDEVE
                     ITAGYDDDIPPPFGCGIVLMPWPNRVRDGRWTYDGRTLQLDITEPARGNALHGLLRNA
                     PYQLVARTACSVTLRAQIFPQNGYPFRLDTTVCYTLVPDGLHVTHTVRNVGSSCAPVA
                     VGAHPFLAIGDVPMDRLTLTVSADRHIDVDDRLNPVGVTPVQGTDWDLREGRLVATLD
                     LDDAWSGLTMSGGGSTHWLSAPDGRTVSLWADEQFGYVQVFITREFPRRGGLITAVAI
                     EPMTAPADAFNSGEGLQWIQPGARWSMSWAIRYHDECHLKAR"
BASE COUNT         2667 a         4711 c         4891 g         2660 t
ORIGIN      
        1 ggccgtccac caggttgccg tacaggtcgc acaccgccat gtcgtcgggc gtgagggcgt
       61 cgtagtccat gccggacggc ttgatcacca tcaggtcgtg accggacacc cgcgccgaga
      121 cgttgccggc ggtccagatc accaactgat acttcgtcaa ctgtgcgtgc agatcgcaaa
      181 cctcgcggcg cagttcggcg acgacagcgc tgatttcgga tgtgacggtc atcgggacgc
      241 tccagctgtt gcgggggtgg atatctcgcg cagcgtgcgc atcattgcgt tgccgcggcc
      301 gaaccagtcg tgcagcttga cgtattcggc atacagccgg tcgtagcgcg cgacgttgtc
      361 aacgatgggg aggtaagcgt tgcgggtacg gccgcccatc tgctttgcag cggcaggcac
      421 gtcctggtat gcgccggcgg ccgcggcggc gtgaatcgca gcgcccagcg cgggcgcctg
      481 tgccgaattg acggtcgaca acggcaatcc cacgacgtcg gcgtagatct gcatcagcag
      541 gcggttcttg aggaggccgc caccgacaac gacctcggtc accggcacac cgttgttcac
      601 gaaggtctcg acgatcatcc tcgtcccgta ggccgtcgcc tccagcagcg cccggtacag
      661 atcggcacac gtggtggcca gggtctgccc gacgatcacc cccgacagct ggtgatcgac
      721 cagcaccgaa cgattgccgc tgtgccagtc cagggcgacc aatccatgct ggccgacgcg
      781 ctgtgccgcg gcctgctcgg tcagtaggtc gtggacggac acgttgcgat cccgggcctg
      841 ctcgtggatg gacccgggga cacagttgtc gacgaaccac gcgaaaatgt cgccgactcc
      901 ggactgaccc gcctcatacc cccaataccc ggcgacgatg ccaccgtcga cgacgccgca
      961 catccccgga acgtcttgga ggacatccga attcatgacg tgacaggtgg atgtgcccat
     1021 gatggccagc agttggccgg gttcgaggga attggccgcg gccgaagtga catgcgcgtc
     1081 gacgttgccg acggcgacgg gcagcccggc gggcaggccc atccactggg cggcttgttc
     1141 ggtcagcact ccggcggcat cgccgagtgc tccgatcgga tggtcgagct tggcgtcgac
     1201 gaaatcggcg aacttgggat ggagcgcggt caggaagtcc cgggtggggt agcgcccgtc
     1261 ctggcggatg cccttatagc cggcgctgca cgcgttgcgc acgtaagacc cgcacagctg
     1321 ccagacgatc cagtcggcgg cctcgaccca gcggtcgatg gcgtcgtaga cctcgggatc
     1381 ctcctcgagg atctgcagcg ccttcgcgaa ctcccactcg ctggatatga atccgccgta
     1441 tcgcttgagc cacggctcat tccgttcggc ggcgagggcg ttgatccgat cggcctgtgg
     1501 ctgtggagag tgatgcttcc acagcttggc gtatgcatgc gggcggtcgg cgaaggccgc
     1561 cgtctcgcac aacggtgtgc cgtcggcgag caccgggatc atggtgcagg cggtgaaatc
     1621 ggtgccaatg ccgatcacgt cgggcgggtc gatgccggct cggctgatcg cctctggtac
     1681 agcaattttc agtacacgtc ggtaatcctc gggcacctgc agcgcccatt gggcgggcag
     1741 gcgcacgcca ccaaccggca gcgactgagt caatacgccg tgttcgtaaa tgtgctcggc
     1801 gctggccagt tcctctccgt catctactcg aacgacgccg gcgcgtccgg agagagtgcc
     1861 gaaatcgatt ccgatcgtgt acttctggcg gtgcatcaag atcctcactt ggtcctggta
     1921 tgcgggcgag tgctatcgcg aacgacgagt tccggagcga gcagcggagt ggccggcgac
     1981 accccgtcga ggacggcggt gagaagttcg atcgcgcgtt ggccgaccgc gtggaagtcc
     2041 tgtcgcaccg tgctcagcgc gggattgagg taggcggcct cgggaacgtc gtcgaacccg
     2101 atcaggctga cgtcgtcggg cacagcgata ccgccctgtg cgaacgcgtg caggatgccg
     2161 atcgccatct ggtcgttggc aacgaagacg gccgtgaagt cgttgcgctt gaccaattct
     2221 cggccgatcg cataaccgcg agccgggctc cagtcacctt ccaggtcggc ccgcaccggc
     2281 agccgggcgg cgcgcatgac ctcgacgtag ccggcccggc gggcacgggc ctcggtccag
     2341 gtcaacgggc cgctgacatg gacgatatcg cggtgaccga ggtcgatgag atgctgtgtc
     2401 gccagccgtg cgccttccat ttggtcgaca ccgacgctca gtccgcgccc cgacagctca
     2461 ccctcgacga cgatcaacgg gatggtggtg gcctccgacc gcaccacggc gagcgcctcc
     2521 tgttgggtga cgatcatgac gatcgcctcc accgactggc ggctgagata gttcagcgct
     2581 ccgcgcaact cgtcgtgggt gaccgcggcc agcgtcacca ggctggagaa gtagcccgcg
     2641 gcgcgcgctg cgtcctgaat cgagaactga atgctggccg gcccgtaaag cgcggtgttg
     2701 ctgccgatga tcccgatgat cttggatctt ctggtgacca acgcgcgcgc ggccgtattc
     2761 gggtgatatc cgagatcggc gatcgcgcgc tccacccgtt cccgcgtgct gggccggatg
     2821 ctcgccgagc cgttgatgac ccgcgacacc gtctgatgcg agacgcctgc gagtcgggcg
     2881 acgtcggcca tcacgggtcg gcgcggaatg ggttctgccg tcatcgggtg ttcctgacca
     2941 ggacgcgctg tacgaggacg aagaccagca gcaggatgcc cgtcatgatc cgggtccagt
     3001 acgaatcgag attctcggcg gtgacgaaca cctggatgat gccgaggacg agcactccga
     3061 tcagcgaccc cagcacgaaa ccgactccgc cggagagcaa cacaccgccg atcaccgccg
     3121 ctgcgatcgc gtcgagctcg aggccgacgc cgttgagact gtagccggac agcttctgca
     3181 cgccgagcaa caacccggcc aacgcggcgc agaaaccgct gatcacatac accgagatct
     3241 gggtacgcgt gacggccagc cccatcaact gggccgactg ccggctgccg ccgaccgcgt
     3301 atacggttcg cccgaaccgg gtttcgtgca acacaaagac cgcggctacc acgacgatca
     3361 gtgcgatgac caccgtccag cggatgaact tgtcctcata caggtacacg tagttcagcc
     3421 cgagcgcccg caacaccgga tccttgatcg gcaggctgtt gacgctgatg acgtaacaca
     3481 gcccgcgcgc gaggaacatg ccggcgagcg tcacgatgaa tggctgcagc tcgaagaact
     3541 cgatcacggt gcccatcagc aagccgagca gtggaccgac aattagcacg gtcgcgatcg
     3601 cgatcggtgc gggccaacca gattgaagcg tcttcgcaag gatcaccgtg gacagtgcga
     3661 cgaccgagcc gacgctcaga tcgatcccgc cggtgaggat cacgaacgtc atcccgacgg
     3721 ccagcacgat caggtgagcg ttgtcgacga gcaggttcaa gaacacctgg gcggggctgg
     3781 cgaagtcgta gcgcacgatg acggtggtga acaacgccac gaagaggatc acggaggcaa
     3841 tgggggagag atagcgtccg cgtatgcgct cggccaccgc cggggcgatc gggtggcgac
     3901 gcagggttgg ggtgctcacg atgccgccac cttgtccgct cgctgtgccg gttcggcgac
     3961 ttcgggttcc gggactgcgg gctgcagcgc ggcgcgtcgc cgtcgtcgaa gcagtcggcg
     4021 gaaccgcggt gcttgcagca ggcagaccgc gatcactact accgccttga agaccagcgt
     4081 cgtctccgga gtgatgcccg cggtgtagac ggttgtcgtc agggtttgaa tgatcaacgc
     4141 gcccaggatc gtccccgtca ggctgaagcg gccgccgagc agggacgtgc ccccgatcac
     4201 caccgcgagg atggcgtcca tctcgatcca cagtcccgcg ttgtttgcgt ctgcggcaga
     4261 gatgttggag gcgatcatca accccgcgat gccggcgcac agcgcgctga acacgtacac
     4321 ggcgaatacg atcgtgcggt accgcacgcc ggccagccgg ctggcggcgg gattgacccc
     4381 gacggactcc agcagcatgc caagtgcgct gcgccgagtg atcagtccga caacggcgaa
     4441 tactgtcagg ctcgtcaaga tcgccaccgg tagaccgagg gcgtagcccg cgccgaggac
     4501 cttgaatggc tcgctggtta cggtgacgat ctggccttcc gtaatcagca gcgcgatgcc
     4561 gcgaccggcc gtcatcagca caagggtggc gataatcggc tgaatcccga agatggacac
     4621 cagcattccg ttccacaggc ccagaacaac ggcgacgccc agcgcaatcg acatagcggt
     4681 gacgatggtg gccacgctgc ccgggtctgc cgcggaggca atgtgcgcgc atgcgagcgc
     4741 cccggagatc gccaccaccg caccgaccga caggtcgatg cctcgagagg cgatgacgag
     4801 agtcattccg agcgcgacga gcatggtcgg tgcgccgttg cgcaggatgt cgatgaggct
     4861 gccgtagagg tggccgtctt cgatccgaac ggtcaagaac gcgggagtga gtacgacgtt
     4921 gaggcccagc aacgcaatca gtgccaaggt cggccacagc agcggcgagg cggtcaggcg
     4981 acgcatcatc aatggctccc gctggcgatg agccgggtga gctccgaaac cgcgcagccg
     5041 ccgtcgatct gggcgatgtt gtgtccgtcg cgcattacgg cgacgcgatc actgatccga
     5101 gccacctcat cgagttcggc cgagatgaac agcactgcca tgccgtcggt ggccagatcg
     5161 gtcaccagtt tctgaatctg cgccttggcg ccgatgtcga tgccgcgagt gggctcgtcg
     5221 aggatgagca gtgtcggccg ggtgatcagc cagcgggcca gcagcacttt ctgctgattt
     5281 ccgccgctga ggttcttgat gagcacatcg gggtcgcggg gacggacgtc gagcgcttcc
     5341 atatagcgat gcacgagttc gcttttggcc ttcgccgaca aaggtcgggc gaagccccgg
     5401 cgtgcctgca acgccaacac caggttgtcg cgaacgctga gttcgccgac gatgccctca
     5461 ccctttcggt tctccgaggt gaacgcgaac ttgtgagcga ttgcagcgcg cggtgagcgc
     5521 aatcgggccg gtttgccgtc gacggcgatc tgcccgctcg ttgccttgtc cgcgccgaac
     5581 agcaagcggg cgaactcagt tcggcccgaa ccaagcagcc cggccaggcc gaccacctcg
     5641 ccggggaaga tgtcgagggt tatcggatcg acccgcggcc tgcggcagac atccacggcg
     5701 cgcacgattg gcgggacggt gtcgtcgcgg tgtgcgcccg gctcggcgtc ggcgatctcc
     5761 tcgagtacat ccagctcccg gcccaacatt gctgcgatca gcgcatggcg atccaggtca
     5821 gcggtgaggt attcaccgac cctgcgaccg ttgcgcagca ccgtcattcg gtccgagatc
     5881 tcatacacct gatcaaggaa gtgcgaaacg aacaggaccg ccgtcccgcc gtcgcgaagc
     5941 tgccgaatca cccggaagag ttcggcaact tcctctgcgt ccagactcga cgtcggttca
     6001 tcgagaatca gcacacgcgc cgaaacggcg gtggcgcgcg cgatggcaac gagttgctga
     6061 atggcgatcg gatgggcgcc gagcgttgag cgcgggtcga tgtcgagttc cagctgcgac
     6121 agcagttcgt gggcgcgacg gttcagcgca cgatggtcga ttcggccgag cctgcgcggc
     6181 tcgcgcccca gcaggatgtt ctctgcgacg gtgagattcg tgcacagatt gacttcctga
     6241 tagacggtgc tgatcccggc gtcctgcgcc tgccgtggcc cggtgaaacg gcttggcacg
     6301 cccgccaccg tgatggtgcc cgtgtcgatg tcatacacgc cggtgagcgc cttgatcaga
     6361 gtcgatttac cggcaccgtt ctcacccatg agggcatgca cttcgccggg cagcagccgg
     6421 aacgagacac cgtccagcgc cttgactcca ggaaagctga tgctgatgtc gcacatctca
     6481 accaccggag cctgcggctc tgcggtggat gtcggtgcca ttgcctactc gctttccggt
     6541 ggtggaactt ggcgccgccc cgggttttgg ggttgggcca ctccggggcg gcgccaaggg
     6601 tcttcagagg tgttgtcagt acttacggtc cggcagcgca gcgacggctt gctgctggtc
     6661 gaacgtcttg tcgggcacca caactcgcgg agccaccgtt tcgcctgcca ctaccttctt
     6721 ggccaggtcc atcagttgcg ggccaagcag cggattgcac tcaacgacga agttgaactt
     6781 gccgtcggcc agggcctgca tgccgtcgtg ggtggcgtcg accgcgacga tcttgacgtc
     6841 ttgccctggt ttgagcccgg ccgcctcgat ggcttcgatc gcgccaaggc ccatgtcgtc
     6901 gttctgggcg aacacgacat cgatcttcgg gttggccttg agaaaggcct ccatcacctg
     6961 cttgccgccg gaccgggtga aatcgcctgt ctgcgaggcg attaccttca ggttggggtt
     7021 ggccgcgatg gcctccgaaa agcccttgct cctgtcgatg gcggggtcgg cgccggtggt
     7081 gccttcgagt tcgacgatat tgaccggccc tggcgccgtc gcatattgct tcacgaccca
     7141 gtcgccggcg ctgcggcctt ccgcgacgaa gtcggcgccg acgaaggtct tgtagacgtc
     7201 ggtctcctga gtatcgaccg cgcggtccgt gagaatcacc ggaatgccgg cgttcttggc
     7261 ctcgagcagc actgcgtccc agcctgtccg caccaccggg ctgaaggcga tgacatcgac
     7321 gtgttgctgg atgaacgaac ggatcgccga aatctggttc tcctgtttcc cattggcgtc
     7381 ggagaacttc aggtcgatgc cggcggccgc cgcggactcc tgaatgttct ttgtgttggc
     7441 tgtgcgccag ccgctctccg cgccgacttg agaaaagccc atggtgatct gcccgtcctt
     7501 tgatccgcca ccgccaccgg gggcgggtcc gctgccgcag cccaccatcg ccagggcgat
     7561 cagcgctccc agcgacgttg caaggatttt cttcaccgcg actcctcatt tgtcggttgt
     7621 cgaaatcggt cgctgcgggc caggtcggca tgggttcgta ccgcacctgc gggtgtgacc
     7681 ggcgttacaa agccaggatg ttagcgttaa caaaccgcgg gtgtcaacgg tctgcgccaa
     7741 ccaccgccgc cgctaggccg tcagctgttc gcgagctgcg cgcctgacaa ctccgcacgt
     7801 cagaggccag atctcaaccg gttgttgaca agccagtggc gtggctcaca tactaacgct
     7861 ccgagatgtt aacgctcaca ttctcgatcg gctgcgtccc gacgaagccg tgtccactca
     7921 ggaggatttt cgtgaggaaa ttgatctggc tcgccgccct attggcgatg ctgaccacgc
     7981 tggctctctc gggctgtggc cggtccaccg acaccgccgc gggcggcaac ggagacaagg
     8041 gcaccatcgg tatcgcgatg ccaaccaaat cgtcagaacg ctgggtcgcc gacggcaaga
     8101 acatggtgga ccagttccag tcgttgggtt acatgactga tctgcagtac ggcgacgacg
     8161 tcgtccagaa ccaggtgtcg cagatcgaga acatgatcac gaagggcgtg aagcttctcg
     8221 tcgtcgcgcc gatcgacggt tcgtcactga ccacaacgct gcaacatgcg gcggacgcga
     8281 agatcccggt gatcagctac gaccgactga tcaagggcac accgaacgtc gactactacg
     8341 cgacgttcga caacttcaaa gttggtgtgc tgcagggcag ctacatcgcc gacaagctcg
     8401 gtctcgccca aggcaaaggc ccgttcaata tcgaattgtt cgccggttct cccgacgaca
     8461 acaacgcaac atacttcttc gacggcgcca tgagcgtgct caagccgtac atcgacagcg
     8521 gcaagctcgt cgtcaagagc ggccagacct ctttcgatca gatcgccaca ctgcgctggg
     8581 atgccggatt ggcccagtcg agaatggata acctcctcag tcaggcctac acgacggcca
     8641 gggtcgacgc ggtgctgtcg ccctacgacg ggatctcccg tggcgtcttg tcggcgctga
     8701 agagtgcggg ctacgggaac gcggcaaaac cgctgcccat cgtgaccggt caggacgcgg
     8761 agttggcgtc ggtgaaatcg atcgtcgcag gtgagcaaac ccagacggtc ttcaaggaca
     8821 cccgtgagct ggcgaaggcc gctgtgcagg aagccagcgc ggtcctcacc ggcgggaagc
     8881 cggaggtcaa cgacaccaat acatacgaca acggcatcaa ggtggtgccc tcgtatctgc
     8941 tccaaccagt cagcgttgac aagtcgaact accagtcggt gctgatcggt tcgggttact
     9001 acaccgaagc ccaggttcag tgacctcaac gcttcttgag atgcggggca tcaccaagga
     9061 gttccccggt gtcaccgcac tctccgaggt ggatctgacg gtgaaagccg gtgccatcca
     9121 tgcgatctgc ggcgagaacg gcgcgggcaa gtcgacgttg atgaaggtcc tcagcggtgt
     9181 gtatccgcac ggggactaca gcggcgacat tctgttcgac ggacacgcat gcgagttcaa
     9241 ggacatccgg gccagcgagc gcgccggtat cgcgatcatc caccaggagc tcgcgctggt
     9301 gccctacttg tcgattgccg aaaacgtgtt cctgggcaac gaacaaagtc gtttcggcgt
     9361 gatcggttgg gaccaaacgc ttatcgaggc gcaggcgctg ctcgaccggg tgggcctacg
     9421 ggagagtccg caaacccggg tcgctgacat cggagtaggt aaacaacagc ttgtcgagat
     9481 cgccaaggcg ctgtcgaaga aggtacgtct gctgatcctc gacgaaccga ctgcggcgct
     9541 gaacgattcc gacagccgcc atctgctcga tctcatcgtg gatctgcgcg agcatgggat
     9601 cacctcgatc atcatctcgc acaagctaaa cgaggtgatg cacgtcgcgg acaccatcac
     9661 catcctgcgc gacggacgca cgatcgagac gatcgccgtc aacgatgacc tcgccgagga
     9721 tcgcatcgtc cgcggaatgg tcggccgcga tctcacccat cgttttccgc cccgtgcgga
     9781 ccgcgagatt ggcgacatcg ccttctcggt atccaactgg accgtccttc acccaatcga
     9841 tcagcatcgc aaggttgtcg acgacgtctc gctcaacgtg cgcgccggcg agatcgtggg
     9901 catcgcgggt ctgatgggcg caggccgaac cgaactcgcc atgagcgtgt tcggccgcac
     9961 gtacggaaag tatgtgagcg gcaaggtgtt caagggcgac cgggagatca gtacccgcac
    10021 cgtctcggat gcgatccgga acggggtcgc ttacgccacc gaggatcgta agcacctggg
    10081 cctgaacctg atggacagca tcgcccagag catcacgttg ccgtcgttgc ggaagatcag
    10141 caggcggggg gtggtcaatc agcatgagga agccggtgta ggtgagcggt tccgcgcttc
    10201 gatgcggatc aaggcatcat ccgtgtcggc cgttacgggc aagctgtccg gtggcaacca
    10261 acagaaggtc gtgttgagca agtggctgtt caccgatccc gatgtgctca ttcttgacga
    10321 gccgacccgc ggcatcgacg ttggcgccaa gtacgagatc tacacgatca tcaacgaact
    10381 cgccgcacga ggtaaggctg tgctggtcat ctcatcggaa ctgcctgagc tgcttggtat
    10441 ttgcgatcgc atatacacac tcagtcaggg ccggctgacc ggcgaagtgg cacaagccga
    10501 tgcgactcag gagaccctca tgcgccacat gatgaaagga cgggcctagg tgaccaccac
    10561 cgtcgagcaa tcgtctcaga agcccgaggc agggcaacag cccggtgagg catcaccaac
    10621 accaccgggc ctgcgtgagc gggtgtcgtc attcctcatg tcgcacgtgc gccagtccgg
    10681 catggtggtg gcgctggcgg caatcgtgtt gctgttccag gtctggaccg gcggcattct
    10741 gctcaagccg ctgaatgtca ccaacatcgt ccagcagaac ggctacatcc tcgtcctggc
    10801 gatcggcatg gtcgtcgtga tcatctcggg ccacatcgac ctatccgtcg gatcgatcgc
    10861 aggcttcatc ggggcgatgt ccggcgtcct gatggtgcgc aacgacatgc cgtggcttct
    10921 ggccgtcggg ctgtgcgtgg ggatcggcgc gctcatcggt gcctggcagg gattttggat
    10981 cgcctacgtc gggataccgt cgttcattgt caccctcggc ggaatgctcg tcttccgcgg
    11041 cgccacccag tatctgctcg agggacaatc catcgccccg atgccgcacg gcctggaaca
    11101 agtcagcagt ggtttcctgc cggagatggg aaccaacgcc gtctaccact ggccaacggt
    11161 catcctgggt gccgtcatca tggtggccgc ggtggtggtg cagatccgtc gcaggcgctc
    11221 ccaacttcgg tacggccaag aggtctcggc cacaccgcta ttcgtggcga aatgcgcggc
    11281 gatcgtcgcc gcgctcgcgg tgttcacgct attgctggcc agttaccggg gagtgccgat
    11341 cgtcgggatc atccttgtgg tgctgactct gatctacgcg ttcgtcatgc gcaacacggt
    11401 gctcggacgt caggtgtacg ccgtcggcgg aaactccgca gcagcacgcc tttccgggat
    11461 ccgcaaccaa cgtgtcacct tgctggtctt cgtcaacatg ggcgtcctcg ccgctatcgc
    11521 aggactgctg tttgcggcgc gactgaactc cgccacgccg caggcgggca tcaacttcga
    11581 actcgaggcc atcgccgcgg cgttcatcgg cggcgcgtcg tccagcggcg gtgtcggcac
    11641 tgtattcggt gcgatcgtcg gcggattggt gctcggggta ttgaacaacg gcatgtcgat
    11701 catgggcatc ggtagcgacg tccagcaggt catcaaggga ctcgtcttgc tcgccgcagt
    11761 tggattcgac gtctacaaca agcgtaaggg cggacgctag gccgactgcc gttgggtgct
    11821 ctgccgtatg acaagttggg cgggcactag agtcggctga tcgtcgatag gcccgctgtc
    11881 gctgtcttcg attcgggcga tgagcatttc gacactgctg cggccgaccg ttgcgaagtc
    11941 ctggcggacg gtggtcagcg gcggcgagta gaactcggct tctggaacat cgtcgaagcc
    12001 ggccaccagt acgtcttcgg gtacggcgcg accggcttcg gcaaatgcgc gcaacaggcc
    12061 gagcgccatc tgatcgttgg cgacgaagac tgcctcgacg tcggcgcgtc ctgcgagtgc
    12121 gattccggct tgatatcccg atgcagcgga ccaattgccg tgcagcggtt cgggaatggt
    12181 ccgcccggcg gcggccagct cctggcgcca cccggccagg cgggcgtcgg tttccgtcca
    12241 gccttcggca ccggtcacgt gccacactgt cgacgcgcct tgctcgagca ggtgccgggt
    12301 aacctgtcgc gccccgtcga attggtccac gctgaccata ggcagtccca gtccgccggt
    12361 gccgtcgacg gccacgacgg gcatctcgcc gcggagccgg gacagggcgt ggcgcgtttc
    12421 ggaaagcggc gcgatcacca ccacaccctc gaccgactgt tcggccagcc ggttcagcgc
    12481 cctgggcaac gtggtggagt cgatggtcga ttcactggcc acgctgacga aatagccggc
    12541 gctgcgggcg gcgtgctcga tggcgtagac catgctcgct ggtccgtaca gagtgctgtc
    12601 aaagcagatg acacccaacg tctttgaccg atgagtcgcg agggcgcgcg ccgcgagatt
    12661 gcggtgatag cccaattcgt cgatggcggc gttgacccgg ctgcgggtgg actccttcac
    12721 cgaggccgag tcgttgagca cgcgtgacac ggtctggtgg gaaaccccag ccgctttcgc
    12781 gacgtcgaac atgttggccg gtcggcgctc gaccctttcc cccatcggag tgacctcctc
    12841 ttgtgctcgc atagtagtcc ggatgtgagc gctcacgcgg ctttgacgga ccgaatgtta
    12901 gcgctaacat ttgggcatgc aggaagtgat gaccgtcgcg cgactccacg gcgtgcgtga
    12961 catccggacg cacaccgagt cagttccggc cgcggccgca ggagaggaac tggtccgcgt
    13021 caccgccgtc ggcctgtgcg gatccgactt gcactggttc accgaagccg gcatcggcga
    13081 cgcccggctc gtccggccgg tggtgcccgg acatgaattc gccggagtta tcgagggcgg
    13141 cccgcgtcac ggccagcacg tggcggtgga tccggcattg ccgtgccggc gctgcgaaat
    13201 gtgtctcggc ggctatcgca acctctgccc cgatgttgtc ttcgccggtc acggcggcca
    13261 ggatggcgga ctgcagcaat acctggtctg gccctcatgg gcgcttcatc cactgcccga
    13321 cggactgagc ggggtcgacg gtgcgatgct cgagccgctc ggtgtcgcgc tgcacgctct
    13381 ggacttgggc cacttgcgaa ttggatcgac cgtcgccgtc gtcggcgccg ggccgatcgg
    13441 ccttctgttg atgcaggcag cgaagggggc cggggccgtc cgcgtcgtcg tggtcgaacc
    13501 gcttgcgcac cgccgagacg ccgcagtgcg gcttggcgcc gacgccgtgc tcagtgtggg
    13561 tgaggcggcc gacgacgttg gcgccgacgc ggtgtttgaa cttgccggca cggacgctgc
    13621 ggttgagatc gcgctgcggg ctgctcggcc aggcgccagg gtggtgctgg gcggaattcc
    13681 cgacggtgac cgcacgtcgt tccaagcgtc cctggcgcgc cgcaaggggc tcacgctcgt
    13741 cttggtcagg cgcatgaatg aggcttatcc acgggcgatt cggctggcgg cgcaagggtt
    13801 gatcgacgtc tcatcgatcg tgacgcagcg gtacccgctc gagcgcgccg cggaagcgtt
    13861 cgccgtggct gccgaccggc aaggcctcaa ggtcgtcatc gagccttgag gtggcattcg
    13921 tcgtggtacc ggatcgccca cgacatggac caacgagcgc cgggctggat ccactgcagc
    13981 ccctcgccgc tgttgaaggc gtcagcaggt gcggtcatcg gttcgatggc gaccgcggtg
    14041 atcaacccgc cgcggcgagg aaactctcga gtgatgaaga cctgcacgta gccgaattgt
    14101 tcgtccgccc aaagcgatac ggtgcgccca tcgggcgcgc tgagccagtg ggtgcttccg
    14161 cccccgctca tcgtcagacc agaccatgca tcgtcgaggt ccagtgttgc caccagccgg
    14221 ccctcgcgca ggtcccagtc ggtgccctgg accggcgtca cgccgaccgg attgagccgg
    14281 tcgtcgacat cgatgtggcg atcagcagac acggtcaagg tgagcctgtc catggggaca
    14341 tcaccgatcg ccaggaaggg gtgagcgccg accgcgactg gggcgcaaga ggatccgacg
    14401 ttgcgcacgg tgtgcgtgac atgcaacccg tccgggacga gggtgtagca caccgtggtg
    14461 tccagccgga atggatagcc gttttgcgga aagatctggg cccgcagggt cactgagcat
    14521 gcagtgcgcg cgacgagctg gtaaggggca ttgcgcagga ggccatgcag tgcattgccg
    14581 cgcgcgggtt cggtgatgtc gagttgcagc gtccgcccgt cataagtcca ccgcccgtcg
    14641 cgcacgcggt tcggccacgg catcagcacg attccgcagc cgaacggcgg gggaatgtcg
    14701 tcgtcgtagc cggcggtgat ctccacctcg tcgactgaca gatgccgtag tgcggcggcg
    14761 acctcggtga tgaccgcacg cacggatcga cccgcggcct cgagggtcag ctcgtactgt
    14821 tcgccgagca cgggccaagg ccggaattgc atgcccaaat gttagcgctc acattggcac
    14881 tgcctggcga aagaccggcc ttcgcgcacc gcggtgacta cattcccta
//
LOCUS       DASRJA010000678        17283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1783726,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000678 DASRJA010000000
VERSION     DASRJA010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 17283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17283
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783726"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VFC01_30450"
     CDS             <1..989
                     /locus_tag="VFC01_30450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HZN83971.1"
                     /translation="QLGTKWRERFASFDDTPVASASIGQVHKAVWSDGREVAVKIQYP
                     GADEALRADLKTMQRMVSVIKQLSPGADVQGVVDELIERTEMELDYRLEADNQRAFAK
                     AYEGHPHFVVPHIVASAPKVVIQEWIEGIPLSHIIREGTTEQRDLMGRRLFELTYDAP
                     RRLEMMHGDAHPGNFMLLPGDKMGVIDFGAVAPLPGGIPVEIGLSTRYALNDDYDNLL
                     TTMQKIGFIQKGEQVSKREMDDMMRQYVEPLEVEVFHYTRKWLQKMTAVNMDRSVAQI
                     KAARQMDLPAKLAIPLRVIASNVAIACQLDAHIPVKALAAELIPGFAEEPAA"
     gene            complement(1011..1292)
                     /locus_tag="VFC01_30455"
     CDS             complement(1011..1292)
                     /locus_tag="VFC01_30455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HZN83972.1"
                     /translation="MSVETCRERTMPSVPCHVGDPNLWFAENPLDLERAKALCADCPI
                     RRECLSAALERQEPWGVWGGEIIERGAIIARKRPRGRPRKDTVESPAAA"
     gene            complement(1438..1635)
                     /locus_tag="VFC01_30460"
     CDS             complement(1438..1635)
                     /locus_tag="VFC01_30460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83973.1"
                     /translation="MLSNNAFTGVGVAGMSRTLHVAEAAATAHPAFWAYDVAGAAAAI
                     PPQPKRRTTAATGKSVDRGTT"
     gene            complement(1737..3854)
                     /locus_tag="VFC01_30465"
     CDS             complement(1737..3854)
                     /locus_tag="VFC01_30465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HZN83974.1"
                     /translation="MSVEASTSRDELIADLDDEQREAVLAARGPVCVLAGAGTGKTRT
                     ITRRIAHLVAAGHVAPHQVLAVTFTQRAAGEMRGRLRVLDDGVGTGSVQAMTFHAAAR
                     RQLSYFWPRVVGNTGWQLLDTKFAVVAQAANRASLSTSTDDVRDLAGEIEWAKASLIS
                     PEGYAAAVAQAGRDIPFDAAKIATVYAGYEKLKARHDGMALLDFDDLLLHTAAAIEND
                     DAVAQEFRDRYRCFVVDEYQDVTPLQQRVLDAWLGSRDDLTVVGDANQTIYSFTGATP
                     RYLLDFSRRFPEAAVVRLERDYRSTPQVVSLANRVIAAARGRMAGSKLHLVGQRDPGP
                     NPTFTEHTDEVAEAAAVAKKIKNLIESGTAASEIAVLYRINAQSEVYEEALTEAGIPF
                     QVRGGEGFFSRQEIRQALLALQRASEKDAARDSEKLPDIVRAVLEPLGLTAEPPPGTR
                     ARERWEALGALAELVDEEVALRPSLDLRALLTELRQRADARHPPTVQGVTLASLHAAK
                     GLEWDAVFLVGLADATLPISHALAHGPDSEPVEEERRLLYVGITRARTHLALSWALAR
                     APGGRQSRRPSRFLNGIAPQSPSDATPNRPRRPRGATPRCRVCNNTLTTPPAIMLRRC
                     ESCPSDIDDELLAELKDWRLRISKEMSVPAYVVFTDNTLIAIAETLPTDDAALVAIPG
                     IGARKLEQYGPDVLAMVKGRQRS"
     gene            3937..4176
                     /gene="mrx1"
                     /locus_tag="VFC01_30470"
     CDS             3937..4176
                     /gene="mrx1"
                     /locus_tag="VFC01_30470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycoredoxin Mrx1"
                     /protein_id="HZN83975.1"
                     /translation="MSALTMYTTSWCGYCFRLKKVLKAEGIRFKEVDIEADPAAAQFV
                     ASVNEGNQTVPTLKFADGSTLTNPSGAEVKAKLAS"
     gene            complement(4173..5102)
                     /gene="nudC"
                     /locus_tag="VFC01_30475"
     CDS             complement(4173..5102)
                     /gene="nudC"
                     /locus_tag="VFC01_30475"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HZN83976.1"
                     /translation="MTRGFQLRNVPLLSRVGADRADALRTDVDAAIAGWPDALLLRVD
                     RRNQVLISGGQVVLGTAAKLGDKPPENAVFLGRLVDGRHVWAIRAALEGPEDPHAETE
                     VLDLRRAGQIFDEVSAQLVATATALLNWHDNARFSAVDGAPTKSIKGGWARVNAANGH
                     EEFPRIDPAVICLVHDGHDRAVLARQTVWPERLFSLLAGFVEAGESFETCVAREIAEE
                     IGLTVTDVRYLGSQPWPFPRSLMVGFHALGDPEQEFSFNDGEIAEAAWFTRAEVREAL
                     DHGDWSSDSESRLLLPGSISIAREIIESWAALG"
     gene            complement(5099..6202)
                     /locus_tag="VFC01_30480"
     CDS             complement(5099..6202)
                     /locus_tag="VFC01_30480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HZN83977.1"
                     /translation="MATGKLRSRLAAIEENLTARPDAALTDILRIPEPFVSPTQRIVR
                     RIVYATLALLAAVLIVYLDRHGYRDVRDEIGHGQASGELSFLDCFYYATVSLSTTGYG
                     DITPVTPSARLVNVLVITPLRIAFLIVLIGTTVETLTTQSRQALKIQRWRSRVRNHTV
                     VVGYGTKGRTAVAAMVGDEVAPADIVVVDENVSALERARSAGLVTVHGDATKSEVLRL
                     ASAQHAKSIIVATDDDASAVLVTLTARELAPKAKIIAAAREAENQHLLQQSGADSTVV
                     SSETAGRLLGIATQTPSVVEMMEDLLTPDAGFALAEREVSPKEEGGSPRHLTDIVLGV
                     VRNGELLRVDAPEVDALELGDRLLYIRSAEAER"
     gene            6269..6643
                     /locus_tag="VFC01_30485"
     CDS             6269..6643
                     /locus_tag="VFC01_30485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016341756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83978.1"
                     /translation="MQQIASPAPAYRLAALLLGSGVLHLVMPKPYDYIVPTELPGSQR
                     LYTYASGVAELASGALLLSPRTRRLGALSAVAVFVGVFPANINLVRLWRDKPLFMRLG
                     AIARLPLQIPMVTSALKVYRNS"
     gene            complement(6640..9873)
                     /locus_tag="VFC01_30490"
     CDS             complement(6640..9873)
                     /locus_tag="VFC01_30490"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZN83979.1"
                     /translation="MTPRYSPAELASALGLFAPTEEQSAVIAAPPGPLVVIAGAGAGK
                     TETMAARVVWLVANGYASPGEVLGLTFTRKAAGQLLRRVRMRLARLAGAGLVPGCGDM
                     ADDPATVSTYHAFAGNLLREHGLLLPVEPDARLLSETELWQLAFRVVCEHPEHLDTDK
                     TPAAVTAMVLRLAGQLAEHLVDTTQLRDTHVELERLVHGLPAGPFQRDRGPSQWLLRM
                     LATQTERTQLVPLIDALHARMRTEKVMDFGMQMASAARLASAFPQVGQQLRERYRIVL
                     LDEYQDTGHAQRVALSSLFGRGVDDGLALTAVGDPIQSIYGWRGASATNLPRFTTDFP
                     RPDGTPAPTLELRTSWRNPPRALHLANAVSAEARRRSVAVRALRPRPDAQPGVIRCAL
                     LTDVAAERDWVADHLARIYHGARRDGSAVPTAAVLVRRNSDTAPMADALAVRGVPVEV
                     VGLAGLLAVPEVADLVAMLRLVADPTAGAAAMRVLTGPRWRLAGRDVAALWRRAVDLD
                     DRPTRDATTEQIVAQAAADADGACLADAICDPGPADAYSTAGHSRIVALGRELTALRA
                     HLTHPLPELVAEVRRVIGIDTEVRAARPVASGWSGTEHLDAFADVVADFAARPGATMA
                     NLLAFLDAAEEVENGLAPAEMSVANDRVQILTVHAAKGLEWHVVAVPHLSGRVFPSTA
                     STRTWLTDAGDLPPLLRGDRATVSEHGVPVLDTSDVNDRKRLSDKISDHKRRLEQRRI
                     DEERRLLYVALTRAEDTLLLSGHHWGASETKPRGPSEFLCELKDIIETSDTAGNPCGV
                     IEHWAPVPADGEPNPLRDKAIEALWPTDPVGVQRKHMDSGAALVTQAMTGALGANTED
                     IDGWAADVDALLAERDRAADPQVPPLPAQLSVSTLVELGRDPDGVPQRLRRRLPTRPD
                     PHALLGTAFHDWVQRFFHAERLFDLDDLPGAVDRDAGDTEDLAELQAAFAVSPWASRT
                     PIDVEVPFDMVLGGRVVRGRIDAIFTDDDGGVTVVDWKTGEPPATPEAKQHAAIQLAV
                     YRLAWAQLHGCPIDQVRAAFHYVRSGRTVTPDALPDADDLAALLDAA"
     gene            complement(9870..13004)
                     /locus_tag="VFC01_30495"
     CDS             complement(9870..13004)
                     /locus_tag="VFC01_30495"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZN83980.1"
                     /translation="MSAPQTELTPTALTEPGMRGVVRVLGGAGTGKSSLLIRAATAHI
                     AAGSEPESVLLLTGSARLGSHARAAIISALLSAGSRAVVREPLVRTVHSYAFAVLRLA
                     AQRNGDPPPRLITSAEQDGIIRELLAGDVEDGDAPPRAGGAPTPAWPGQLRPALSTVG
                     FANELRDLLARCTERGVDPVALQRIGRAHGRPEWLAAGQFAQAYEQIMLLRSAVGMAA
                     PQATVPALGAAELVGAALEAFATDADLLASERSRIKLLLVDDAQHLDPQAARLVRVLS
                     AGAELTVIAGDASQSVFGYRGADPSLLRGDEPAITLTQSLRCAPAIADAITAVARRLP
                     GADAAREVSGAEHRAGSLKVRIAATPHAESALIADALRRAHLIDGVPWSQMAVIVRSV
                     PRLGATLARALVSAGVPVEAPGPSGTLADQPAVRALLTVLDVTAYGLDGDRALSLLTG
                     PIGRVDPVSLRQLRRALRRADGRQPPREFTDLLTDALDNETISLSTDLDRPLRRVRAV
                     IAAARRSVEDGQDPRYTLWQAWHRSGLQRRWLAASERGGLAGVQAHRDLDAVTALFDV
                     AEQYVARTAGASLRGLVDHVEAMGLAPTPRDQQSETDAVAVLSAHAALGREWEFVVIA
                     GLQEGLWPNTVPRGGVLGTQRLVDVMDGVATDVERGLSSRAPLLAEERRLLIAAMGRT
                     RSRLLVTAVDSDSGDESMLPSPFCDELSALATDPDPEPPEPIHAPRVLAPSALVGRLR
                     AVVCAAPDAVEDMVRACAATQLARLAHSAVAGADPAQWYPMTPLSTDEPLWNGDDHVV
                     TLSPSTLQTLTDCPLRWLLERHGGTDGRDVRSAVGSLLHALVAEPGKTENQMLNELEK
                     LWSRLPFDSQWFSDNELARHRAMLSTFLQWRAQTRATLTEVGTEIDVDGTVGPGVRVR
                     GRLDRLERDTAGRLVVVDIKTGKTPVSKDDAQRHAQLAMYQLAVAEGLLSQGDTPGGG
                     RLVYLGKTSAGGAAEREQDPQTEDTRAEWLEQIRQAAAATQGPGFLARVNDGCTHCPV
                     RAMCPAQTAAGRRP"
     gene            13068..13850
                     /locus_tag="VFC01_30500"
     CDS             13068..13850
                     /locus_tag="VFC01_30500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83981.1"
                     /translation="MTDLLHVHRYGPARPPQILAVHGLTGHGQRWQTLATRHLPEFAI
                     VAPDLIGHGRSSWAAPWNINANVSALARLLDAEADGPVVVVGHSFGGAVALNLAAARP
                     DLVAALVLLDPAVALDGGWMRDIADDMFASPDYTDREEARLEKANGSWGEVDPDELDR
                     ELDEHLIEQPNGRAGWRISIPAMLSYWSELARPISLPRSGIPVTLVIATKTDPPYVTD
                     ELLTGLRERPDFTFVEFDCDHMVPQARPAEAAAVIRKQLERG"
     gene            13852..14148
                     /locus_tag="VFC01_30505"
     CDS             13852..14148
                     /locus_tag="VFC01_30505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HZN83982.1"
                     /translation="MAAVTDEQVETVRALVASIPSGRVSTYGDIAEVAGLSSPRIVGW
                     IMRTDSSDLPWHRVIRASGRPAPHLTTRQLELLRAEGVLASDGRINLAEVRHEF"
     gene            complement(14215..15069)
                     /locus_tag="VFC01_30510"
     CDS             complement(14215..15069)
                     /locus_tag="VFC01_30510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02569 family protein"
                     /protein_id="HZN83983.1"
                     /translation="MTVERPPDHVLAAFGLTGAQPVALGSSWEGGWRCGEVVLSMVAD
                     HARAAWSAKIRETLFVDGVRLARPVRSTDGRYVVAGWRADTYVAGTPEPRHDEVVSAA
                     VRLHEATAKLERPRFLTQPPVAPWSDVDVFIAADRAAWEERPLHSLPAGARVSPGTPD
                     GQRSIELINQLAALRRPTKSPNQLVHGDLYGTVLFAGTAAPGITDITPYWRPSSWAAG
                     VVVVDALAWGEADDGLIERWSPLPEWPQMLLRALMFRLAVHALHARSTAAAFPGLART
                     AALVRLVL"
     gene            complement(15502..16680)
                     /gene="moeZ"
                     /locus_tag="VFC01_30515"
     CDS             complement(15502..16680)
                     /gene="moeZ"
                     /locus_tag="VFC01_30515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/sulfurtransferase MoeZ"
                     /protein_id="HZN83984.1"
                     /translation="MSTPLPPLVEPAGELTREEVARYSRHLIIPDLGVDGQKRLKNAK
                     VLVIGAGGLGSPTLLYLAAAGVGTIGIVEFDVVDESNLQRQIIHGQSDIGRSKAESAR
                     DSVREINPLVEVRLHEFRLEPDNAVELFEQYDLILDGTDNFATRYLVNDAAVLAGKPY
                     VWGSIYRFEGQVSVFWEDAPDGLGLNYRDLYPEPPPPGMVPSCAEGGVLGILCASIAS
                     VMGTEAIKLITGIGEPLLGRLMVYDALDMTYRTIKIRKDPATPKITELIDYEEFCGVV
                     SEAAAEAAADSTITPRELKELLDSGKPLALIDVREPVEWDINRIEGAELIPKGAFESG
                     EALAKLQVDRTPVFYCKTGVRSAEVLAIVKKAGFSDAMHVQGGIVAWGKQLEPDMVMY
                     "
     gene            complement(16766..>17283)
                     /locus_tag="VFC01_30520"
     CDS             complement(16766..>17283)
                     /locus_tag="VFC01_30520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HZN83985.1"
                     /translation="EDGIDTTTFGGDDGFARMVSETLANPKSWTHNPQFAFTRIDSGE
                     PDFRVSLSSPMTVREGCGYDIQLEASCYNPAYADQPRVFINEARWVRGAVPFQGDVGS
                     YRQYLVNHEIGHAMGYQRHEGCGQDGGLAPIMMQQTFSTTNNDAARFDPESVKPDGKT
                     CRFNPWPYPIA"
BASE COUNT         2733 a         5858 c         5878 g         2814 t
ORIGIN      
        1 cgcagctggg caccaagtgg cgcgagcggt tcgcctcgtt cgacgacacg ccggtggcgt
       61 cggccagcat cggccaggtg cacaaggcgg tgtggtccga cgggcgcgag gtcgcggtca
      121 agatccagta ccccggcgcc gacgaggcgc tgcgcgccga cctgaagacc atgcagcgca
      181 tggtcagcgt catcaagcag ctgtccccgg gcgccgacgt tcagggcgtg gtcgacgagc
      241 tgatcgagcg caccgagatg gaactggatt accggctgga agccgacaac cagcgcgcgt
      301 tcgccaaggc ctacgagggc catccgcact tcgtcgtccc acacatcgtg gccagcgcgc
      361 cgaaagtggt gatccaggaa tggatcgagg gaattccgtt gtcccacatc atccgtgagg
      421 gcacgacaga gcagcgcgac ctgatgggcc gtcgcctttt cgaattgact tatgacgcac
      481 cgcgtcggct cgagatgatg cacggtgacg cgcatcccgg aaacttcatg ctgctgcctg
      541 gcgacaagat gggcgtcatc gacttcgggg cggtggctcc gctgcccggc gggattcccg
      601 tcgaaatcgg tctttcgacg cgctacgcgc tcaacgacga ctacgacaac ctgctgacga
      661 ccatgcaaaa gatcggcttc atccagaagg gtgagcaggt gtccaagcgc gaaatggacg
      721 acatgatgcg ccaatacgtg gaacccctgg aggttgaggt gttccactac acgcgcaagt
      781 ggctgcagaa gatgaccgcc gtcaacatgg acaggtcggt ggcgcagatc aaggcggctc
      841 ggcaaatgga tctgccggcc aagctggcga ttccgctgcg cgtcatcgct tccaacgtgg
      901 cgatcgcctg ccagctggat gcccacattc cggtgaaagc gcttgcggca gaactgatcc
      961 cgggcttcgc cgaggaaccg gcggcgtaac acgggaagac ccggcagcgc ttacgcggct
     1021 gccgggcttt ccacggtgtc cttgcgtggc cgcccacgcg gacgcttgcg cgcgatgatc
     1081 gcgccgcgtt cgatgatctc gccaccccaa acgccccacg gctcttggcg ttccagcgcc
     1141 gcggacaggc attcgcggcg gatcgggcaa tccgcgcaca gcgccttggc gcgctcgagg
     1201 tcgagcgggt tttcggcgaa ccacagattg gggtcaccga catgacacgg caccgacggc
     1261 attgttcttt cacggcatgt ctcgactgac atgtccagtc ctctgcttcc tggtcgttgt
     1321 ttcgtcggcc atctgtcttt cgatggatcc gtgaccaggt cttcggtgtc cgaatgacta
     1381 aaggccacgg atccttgact gcgggtccgt ggcctttcgg ctaaatcgtg ggctttccta
     1441 tgtggtgccc cgatccacgg acttaccagt cgcggcggtg gtgcggcgct tgggctgcgg
     1501 cggaatggca gcggcggcac ccgcgacgtc gtacgcccaa aacgcgggat gcgccgtggc
     1561 agcggcctcg gcgacgtgaa gggtgcgcga catgccggca acgcctacgc cggtaaacgc
     1621 gttattgctc aacatgattt gggcaccctc ctttcgactc gcggtgtgat cttgaggcta
     1681 agggtaacag gccacactga caagcgattt tctgaccagc ggttttggca tgatctttac
     1741 gacctttggc ggcctttgac catcgccagc acgtcagggc cgtactgctc gagcttgcgc
     1801 gcgccgatgc cggggatcgc gaccagcgcg gcatcgtcgg tcggtagcgt ttcggcgatc
     1861 gcgatcaacg tgttgtcggt gaacaccaca taggccggca cgctcatttc cttcgaaatg
     1921 cgcagccgcc aatccttcag ctcggcgagc agctcgtcgt cgatgtcgga cgggcagctt
     1981 tcgcagcggc ggagcatgat cgccggcggc gtggtcagcg tgttgttgca gacccggcag
     2041 cggggcgtgg cgccgcgtgg tcgtcggggc cggttcggcg tggcgtcgct tggtgattgc
     2101 ggcgcgatgc cgttgaggaa gcgtgacggc cgtcgactct gccggccgcc cggcgcccgt
     2161 gccaacgccc agctgagcgc caaatgcgtt ctggccctgg tgattccgac atagagcaac
     2221 cggcgctcct cctccaccgg ctcgctgtcg ggaccgtgcg ccagcgcgtg ggagatgggc
     2281 agcgtcgcgt cggccaggcc gaccaggaac accgcatccc actcgagtcc cttggcggcg
     2341 tgcagcgatg ccagggtgac gccctgcacc gtcggtggat ggcgtgcgtc ggcgcgttga
     2401 cggagttcgg tgagcagcgc gcgcaggtcc agcgacggcc gcagcgccac ttcctcgtcg
     2461 acgagctcag cgagcgcacc caacgcctcc caccgctcac gggcgcgcgt gccgggcggc
     2521 ggttccgcgg tgagacccag cggttcgagg acggcgcgca cgatgtcggg caatttttcg
     2581 gagtcgcgtg ctgcgtcctt ctcggaggcg cgctgcaacg ccagcagtgc ttggcggatt
     2641 tcctggcgac tgaagaagcc ttcgccgccg cgcacctgaa atgggatacc ggcctcggtg
     2701 agcgcctctt cgtagacctc cgattgcgcg ttgatgcgat acagcaccgc gatctcggag
     2761 gccgcggtgc cggactcgat caggttcttg atcttcttgg ccaccgcggc ggcctcggcg
     2821 acctcgtcgg tgtgttcggt gaacgtcgga ttcggcccgg ggtcgcgctg ccccacgagg
     2881 tgcagcttgc tgccggccat tcggccgcgg gcggcggcga tcacgcggtt ggccagcgac
     2941 accacttgcg gggtggaccg gtagtcacgc tcgagccgca ccaccgcggc ctcaggaaac
     3001 cgccgggaaa agtcgagcag gtagcgcggt gtggcgcccg tgaacgagta gatggtctgg
     3061 ttggcgtcgc cgacgacggt gaggtcgtcg cggctgccga gccatgcgtc gagcacccgc
     3121 tgctgcagcg gtgtgacgtc ctggtattcg tcgacgacga agcagcggta gcggtcgcgg
     3181 aattcctgtg cgaccgcgtc gtcgttttcg atcgcggccg cggtgtgcaa cagcaggtcg
     3241 tcgaagtcga gtagcgccat cccgtcgtgg cgggccttta gcttttcgta cccggcgtac
     3301 acggtcgcga tcttcgcggc gtcgaacggg atgtcgcggc cggcctgggc aaccgcggcg
     3361 gcgtagcctt ccgggctgat caaagatgcc ttggcccatt cgatttcgcc ggcgaggtcg
     3421 cgcacgtcgt cggtgctggt ggacagactc gcgcggttag cagcctgggc cacgaccgcg
     3481 aacttcgtgt cgagcagctg ccatccggtg ttcccgacga cgcgcggcca gaagtacgac
     3541 agctggcggc gggccgcggc gtgaaacgtc atcgcctgca cggagccggt gcccactccg
     3601 tcgtcgagca cgcgcaaccg gccccgcatc tcccccgcgg cgcgctgcgt aaacgtcacc
     3661 gcgagcacct ggtgtggcgc gacgtgtccg gccgccacaa ggtgggcgat gcggcgggtg
     3721 atggtgcggg tcttgccggt cccggcgccg gccaacacgc acaccggacc acgcgcggcg
     3781 aggacggctt cgcgctgctc gtcgtcgagg tcagcgatga gctcgtcgcg cgacgtagac
     3841 gcctcgaccg acatgcggtc catcttggca gggcgctgtg acaaaagccg ggcgcggcgc
     3901 gggcatgttg gcggtatccg ttacgttgaa cgccttatga gcgctctgac catgtacacc
     3961 acgtcgtggt gtggctactg cttccgcctg aagaaggttt tgaaggcgga ggggatccgg
     4021 ttcaaggagg tcgacatcga agccgacccg gccgcggcgc aattcgtggc atcggtcaac
     4081 gaaggcaatc agacggtgcc gacgctgaag ttcgccgacg gctctacgct gaccaacccc
     4141 agcggggccg aggtcaaggc caagctcgcc agttaaccca gcgcggccca ggattcgatg
     4201 atttccctgg cgatcgagat cgagccgggc aacaacagcc gcgactctga gtcactgctc
     4261 cagtcgccgt gatcgagcgc ttcgcggacc tcggcgcggg tgaaccacgc cgcctcggcg
     4321 atctcgccgt cgttgaacga gaactcctgc tccggatcgc cgagggcgtg gaaaccgacc
     4381 atcagcgacc gcggaaaagg ccaaggctgg ctgcccagat agcggacgtc ggtcacggtc
     4441 agcccaattt cttccgcgat ctcgcgcgcg acgcaggtct cgaacgactc gcctgcctca
     4501 acgaaaccgg ccagcagcga gaaaagccgc tcgggccaca cggtctgccg ggccagcacc
     4561 gcgcggtcgt ggccgtcgtg caccaggcag atcaccgcgg ggtcgatgcg gggaaactcc
     4621 tcgtgcccgt tggccgcgtt gactcgcgcc cagccaccct tgatcgactt ggtcggcgca
     4681 ccgtcgaccg cgctgaaccg tgcgttgtcg tgccaattca gcagggcggt ggccgtcgcg
     4741 accagttggg cgctgacctc gtcgaagatc tgtccggcac ggcgtaggtc gagcacctcg
     4801 gtttcggcgt gggggtcctc cgggccctcc agcgcggcgc gaatcgccca cacgtgcctg
     4861 ccgtcgacca ggcggcccag gaatacggcg ttctccggcg gtttatcgcc cagcttggcg
     4921 gccgtaccga gcaccacctg gccaccggag atcagcacct ggttacgccg gtcgacccgc
     4981 agcagtaagg cgtcgggcca gccggcgatg gcggcgtcga cgtcggtgcg cagcgcatcg
     5041 gcgcgatcgg caccgactcg ggacagcagc gggacgttgc ggagttggaa cccgcgcgtc
     5101 atcgctcggc ttccgcgctg cggatgtaca gcagccggtc accaagctca agtgcgtcca
     5161 cctcgggggc gtccacgcgc agcagctcgc cgttgcgcac gacgcccagc acgatgtcgg
     5221 tcaggtggcg cggcgaaccg ccctcttcct tggggctgac ttcgcgttcg gcgagcgcga
     5281 acccggcgtc cggggtcaga aggtcttcca tcatctcgac aacgctcggg gtctgcgtcg
     5341 ctatgcccag cagccggccc gcggtctccg acgacaccac cgtggagtcc gcgccggatt
     5401 gctggagcag gtgctgattc tcggcttcgc gagcggcggc gatgatcttg gccttcggcg
     5461 cgagctcgcg cgcggtcagc gtgaccagca ccgcgctggc gtcgtcatcg gtcgcgacga
     5521 tgatcgattt cgcatgctgg gcgctggcca gccgcaacac ctcggatttg gtggcgtccc
     5581 cgtgcacggt caccagccca gcgctcctcg cgcgctccag ggcggacacg ttctcgtcga
     5641 cgacgacgat gtccgcgggc gccacctcgt cgccgaccat cgcggccacc gccgtcctgc
     5701 ctttggtgcc gtatccgacg acgacggtgt ggttgcgcac tctgctcctc catcgctgga
     5761 tcttcaatgc ctggcgcgac tgcgtggtca gcgtctccac ggtggtgccg atcaagacga
     5821 tcaggaaggc gatccgcagc ggtgtgatca ccaggacgtt gaccagcctc gcggacggcg
     5881 tgaccggggt gatgtcgcca tatccggttg tcgataacga gaccgtcgcg tagtagaagc
     5941 agtcgaggaa cgatagctcg ccgcttgcct ggccatgccc aatctcgtcc cgcacatccc
     6001 ggtatccgtg ccggtccagg tacacgatca gcaccgccgc aagcagcgcc agcgtcgcgt
     6061 agacgatccg gcggacgatg cgctgcgtcg ggctgacgaa cggctccggg atccgcagga
     6121 tgtcggtcag cgccgcatcg ggccgggcgg tcaaattctc ctcgatcgct gcgaggcggc
     6181 tccgcaactt acctgtggcc acgacaactc gtcatcggaa ggacccgttg cacgacacaa
     6241 tgtaatcatg gccgctgtcc cacccgaact gcagcagatc gccagccccg cgccggccta
     6301 ccgcctagca gcgctgttgc tcggctcagg tgttttacat ctcgtaatgc ccaagccgta
     6361 cgactacatc gttcccacag aactgccggg cagccagcgg ctttacacct atgcctcagg
     6421 cgtggctgag ctcgcaagcg gcgcgctgct gctttctccg cgcactcgaa ggctgggcgc
     6481 actgtcggcg gtggccgtgt tcgtcggggt gtttcccgcc aacatcaact tggtgcggct
     6541 gtggcgcgat aagccgttgt tcatgcggtt gggcgcgatc gcgcggctgc cgctgcagat
     6601 cccgatggtc acctcggcgc tgaaggttta ccgcaactcc taggcggcgt ccagaagtgc
     6661 agccaggtcg tccgcgtccg gcagcgcgtc gggagtgacc gtccgcccgg accggacgta
     6721 gtgaaatgcg gcgcgcacct ggtcgattgg gcacccatgc agttgcgccc acgccagccg
     6781 gtaaaccgcg agctgaatcg ccgcgtgctg cttggcttct ggggtggctg gaggttcgcc
     6841 cgtcttccag tccaccaccg tgacgccgcc gtcgtcgtcg gtgaagatcg cgtcgatgcg
     6901 gccgcgcacc acgcgtccgc cgaggaccat gtcgaacggg acctccacgt cgatcggtgt
     6961 gcgggacgcc cacggcgaca ccgcgaaagc ggcctgcagc tcggcgagat cttcggtgtc
     7021 gccggcgtcg cgatcgacgg cgccgggcag gtcgtcgagg tcgaacagcc gctcggcgtg
     7081 gaagaaccgc tgcacccaat cgtgaaatgc ggtgcccagc aaggcgtgcg gatccggccg
     7141 ggtcggcaga cgccggcgta gccgttgcgg gacgccgtcg gggtcgcgac cgagctcgac
     7201 cagtgtgctg accgacagtt gagcgggaag cggtggcact tgcgggtcgg ccgcgcggtc
     7261 acgctcagcc agcagggcgt cgacgtcggc cgcccatccg tcgatgtcct cggtgttcgc
     7321 gccgagagcg cccgtcatcg cctgcgtcac cagcgccgcc ccgctgtcca tgtgtttcct
     7381 ctgcacaccg acggggtcgg tcggccagag cgcttcgatc gccttgtcac gcaacggatt
     7441 cggctcacca tcggctggta ccggtgccca gtgttcgatg acgccgcagg ggttgcctgc
     7501 ggtgtccgac gtttcgatga tgtccttgag ctcgcagagg aattcggagg gaccgcgggg
     7561 tttggtctcg gaggcgcccc aatggtgacc cgacagcagc agggtgtcct cggcgcgggt
     7621 gagcgccacg tacaacagcc tgcgctcctc gtcgatgcgg cgctgctcga gacggcgttt
     7681 gtgatcggag atcttgtcgg acaatctctt tcgatcgttg acgtcggatg tgtcgagcac
     7741 cgggacgccg tgctcggata ccgtggcgcg gtcgccgcgc agcagcggcg gcagatcgcc
     7801 tgcgtcggtg agccacgtgc gtgtcgatgc cgtcgacggg aacacccgcc cggacaggtg
     7861 cggcaccgca accacgtgcc actccaaccc cttggccgcg tgcacggtga ggatctgcac
     7921 ccggtcgttg gcgacggaca tttcggcggg cgccaggccg ttctccacct cctcggcggc
     7981 atccagaaac gccagcagat tggccatcgt cgcgcccggc ctggcggcga agtcggccac
     8041 cacgtcggcg aacgcatcca ggtgctcggt gcccgaccag cccgatgcga ccggcctcgc
     8101 ggccctgacc tcggtgtcga tgccgatcac ccggcgcacc tcggcgacga gctcgggcag
     8161 cgggtgcgtc aggtgggcgc gcagggcggt cagctcgcgg cccagcgcga cgattcggct
     8221 gtgtcctgcg gtcgaatacg catcggccgg gccgggatcg cagatcgcgt cggctaggca
     8281 ggccccgtcg gcgtccgcgg cggcctgcgc gacgatctgc tcggtggtgg cgtcgcgcgt
     8341 cggccggtca tcgaggtcta cggcacgtcg ccacagggcg gcgacatcgc ggcctgccag
     8401 tcgccaccgc ggccccgtga gcacccgcat ggcggcggct cctgcggtgg gatcggcgac
     8461 caaacgcagc atcgccacca ggtcggcgac ctcgggtacc gccagcaacc cggcaaggcc
     8521 gacaacctca accgggacac cgcgtaccgc aagcgcgtcg gccatcggcg cggtgtcgga
     8581 gttgcgacgt accagcaccg ccgcggtcgg gactgcagac ccatctctgc gggcgccgtg
     8641 gtaaatccgc gccaaatggt ctgcgaccca atcccgttcg gcagcgacgt ctgtcagcaa
     8701 tgcgcaccgg atcacacccg gctgggcgtc aggccgcggc cgcagcgccc gcaccgccac
     8761 cgaacggcgc cgcgcctcgg ccgacaccgc gttcgcgaga tgcagcgccc gcggcggatt
     8821 gcgccagctt gtgcgcaact cgagcgtggg cgcaggcgtt ccgtccggcc gcgggaagtc
     8881 ggtggtgaac cgtggcaggt tcgtcgccga cgcgccgcgc cacccgtaga tcgactggat
     8941 cggatcgccg acggccgtca aggccaggcc gtcatcgacg ccgcggccaa acagcgatga
     9001 cagcgccacg cgctgcgcgt gcccggtgtc ctgatactca tccagcagga cgatgcgata
     9061 ccgttcgcgc agctgctggc cgacttgcgg gaacgccgac gccaatcggg cggccgaggc
     9121 catctgcatg ccgaagtcca tcaccttttc cgtccgcatg cgcgcatgca gtgcgtcgat
     9181 cagcggaacg agttgggtgc gttcggtttg ggtggccagc atgcgcagca gccactggct
     9241 gggtccgcgg tcacgctgaa agggcccggc cggcagcccg tgcaccagcc gctccagttc
     9301 gacgtgggtg tcgcgcagtt gcgtggtgtc cacgaggtgt tcggccagct gccccgcgag
     9361 ccgcagcacc atcgcggtca ccgcggccgg agtcttgtcc gtgtcgaggt gctcgggatg
     9421 ttcgcagacc acgcgaaagg ccaactgcca cagctctgtt tcgctgagca gtcgggcgtc
     9481 gggctcgacc ggcagcaaga ggccgtgctc gcgcagcagg ttgccggcga acgcatggta
     9541 ggtgctgacg gtcgctgggt cgtcggccat gtcgccgcag ccgggaacga ggccggcacc
     9601 ggcgagacgc gccagtcgca tgcggacgcg ccgcagcagc tgacccgcgg ccttgcgcgt
     9661 gaacgtcagg ccgagcacct cgccggggct tgcgtatccg ttggcgacca gccatacgac
     9721 gcgggccgcc atggtttccg tcttgcccgc gccggcacct gcgatgacca cgagcggccc
     9781 tggcggggcc gcaatcaccg cggactgctc ttccgtgggc gcgaaaaggc caagcgccga
     9841 agctaattcg gccggactat agcgtggtgt cacggtcgtc ttccggcagc ggtctgggcc
     9901 gggcacatgg cccgcaccgg gcagtgtgtg cagccgtcgt tgacccgggc gaggaacccg
     9961 ggtccctgag tggctgccgc cgcttggcgg atctgctcga gccattccgc gcgggtgtct
    10021 tcggtctgcg ggtcctgttc gcgttcagcg gcaccgcccg ctgaggtctt gccgagatag
    10081 accaagcggc ctccacccgg cgtatcacct tgtgacagta ggccttctgc gacagcaagc
    10141 tggtacatgg ccagctgagc gtgccgttgc gcgtcatcct tgctgacggg ggtcttgccg
    10201 gtcttgatgt cgaccacgac caaccggccc gcggtgtcgc gttcgagtcg gtccaggcgg
    10261 ccacgcaccc gcactccggg gccgacggtg ccgtcgacgt caatctcggt gccaacctcg
    10321 gtgagcgtgg cgcgggtttg cgcgcgccac tgcaagaatg tcgagagcat cgcgcggtgt
    10381 cgcgccagct cgttgtccga aaaccactgc gaatcgaacg gtagcctgct ccagagcttt
    10441 tcgagttcgt tgagcatctg gttctcggtc ttgcccggct cggcgaccag ggcgtgcagc
    10501 agggacccga ccgcggagcg gacatctcgc ccgtcggtgc cgccgtggcg ctcgagcagc
    10561 cagcgcagtg ggcagtcggt cagcgtctgc agcgtcgatg gcgacagtgt cacgacgtgg
    10621 tcgtcgccat tccacagcgg ctcatcggtg gatagcggcg tcatagggta ccactgggct
    10681 ggatcggcgc cagcgacagc ggaatgcgcg agccgagcta attgcgttgc cgcgcaggcc
    10741 cgtaccatgt cttcgaccgc atccggtgct gcgcacacca ccgcccgcag ccgaccgacc
    10801 agagccgacg gggccagcac gcgaggcgcg tggatcggtt cgggcggctc aggatcggga
    10861 tcggtcgcca acgccgaaag ctcatcgcag aacggcgacg gaagcatcga ttcgtcgcca
    10921 ctgtcgctgt ccacggcggt caccagcaag cggctgcggg tccgccccat ggcggcgatc
    10981 agcagcctgc gttcctcggc cagcagcggt gccctgctcg acaagccacg ctcgacatcc
    11041 gtcgcgacgc cgtccatcac gtcgacgagc cgctgagtac ccaagacgcc accgcgggga
    11101 acggtattgg gccacagccc ttcctgcagt cccgcgatga cgacgaactc ccattcccgg
    11161 ccaagcgccg cgtgcgcgct gaggaccgcg accgcatcgg tctctgattg ctgatcacgg
    11221 ggcgtcggcg caagccccat cgcttcgacg tggtcgacca ggccgcgcag cgacgcgccc
    11281 gcggtccgcg cgacatactg ctcggccacg tcaaacagcg cagtcaccgc gtcgaggtcg
    11341 cggtgggcct gcacgcctgc caggccgccg cgctcactgg ccgccagcca gcgccgctgc
    11401 aggccggacc gatgccaggc ctgccacagc gtgtagcgcg ggtcttgtcc gtcttccacg
    11461 ctgcggcgcg cggctgcgat caccgcacgc acccgccgca gtggtctgtc cagatctgtt
    11521 gagaggctga tggtttcgtt gtcgagcgcg tcggtgagca agtcggtgaa ctcgcgcggc
    11581 ggctggcggc catcggcgcg acgcaacgca cgtcgcagtt gacgcagcga caccgggtcg
    11641 actcgaccga tcggcccggt cagcagcgac agcgcccggt cgccgtcgag accgtacgcc
    11701 gtcacgtcga gcaccgtcag cagcgcccgc acagcgggct gatcggccag cgtaccgctc
    11761 ggccccggcg cctccaccgg cactcccgca gagacaaggg cacgcgccag cgtcgcgccc
    11821 agccgcggca ccgaccgcac gatcaccgcc atctgcgacc acggcacgcc gtcgatcagg
    11881 tgtgcgcggc gcagtgcgtc ggcgatcaac gccgattctg cgtgcggcgt cgcggcgatc
    11941 cgaaccttga gcgagcccgc ccgatgttcg gcaccgctta cctcgcgggc ggcgtcggcg
    12001 cccggtagcc gccgcgcgac cgcggtgatg gcgtcggcga ttgccggtgc gcaccgcagc
    12061 gattgggtga gcgtgatggc gggctcgtcg ccgcgcaaca gcgacggatc ggcgccgcgg
    12121 taaccgaaca ccgactggct ggcgtctccg gcgatgacgg tcagctcggc accggccgac
    12181 agcacgcgca ccagccgagc agcctgcggg tcgagatgct gcgcgtcgtc gacaagaagc
    12241 agcttgatcc tgctgcgctc cgaagcgagc agatctgcgt ccgtcgcgaa cgcttccagc
    12301 gcggcaccga ccagttcggc ggcgccaagt gcgggcacgg tcgcctgcgg cgccgccatg
    12361 ccaacggcgg agcgcaggag catgatctgt tcgtaggcct gtgcgaactg gcctgcggcc
    12421 agccattcgg gccggccgtg ggcgcgcccg atacgttgca gcgcaacggg atccacaccg
    12481 cgctcggtgc agcgagccaa caggtcgcgc aattcgttgg cgaacccgac ggtgctcaac
    12541 gcgggccgca gctggccggg ccacgcaggc gtgggggcac ctcccgctcg cgggggagcg
    12601 tcgccgtcct ccacatcgcc tgcgagcagt tcgcggatga tgccgtcctg ctcggcactg
    12661 gtgatcagcc gcggcggcgg gtcgccgttg cgctgcgccg ccagccgcag caccgcgaac
    12721 gcatacgagt gcacggtgcg gaccagcggc tcgcggacca cggcgcgact gccggcagac
    12781 aacaaggccg atatgatggc cgctcgggcg tggctgccga gccgggccga acccgtgagc
    12841 agcagtaccg attctggctc gctgcccgcg gcgatatggg ccgtcgcagc ccgaatcaac
    12901 aagctgctct tgcccgtacc cgcgccgccc agcacgcgca ccacgccgcg catacccggt
    12961 tcggtgaggg cggtcggggt cagttcggtc tgcggtgcgg acatgagggc atgacaccac
    13021 gggggtccga caagcagccg tcgtcggctg ctggcagcat ggcggccgtg accgacctgc
    13081 tgcacgtgca ccgctacggc ccggctcgcc caccgcagat cctggccgtc cacggcttga
    13141 ccgggcatgg ccagcgctgg cagacgctgg cgacgcgaca tctgcccgaa ttcgcgatcg
    13201 tcgcaccgga cctgatcggg cacggacggt cgtcgtgggc ggcgccgtgg aacatcaatg
    13261 cgaatgtcag cgcgctggcg cgcctgctcg acgcggaggc ggacggaccc gtggtagtgg
    13321 tcgggcattc gttcggcggc gcggtagcgc tgaatctggc tgccgcacgc ccggatctgg
    13381 tcgcggcgct ggtgctgctc gatcccgccg tggcactgga cggcggctgg atgcgcgata
    13441 tcgcggacga catgttcgcc tcgccggact acaccgaccg tgaggaagcc cgactggaga
    13501 aggcgaacgg ctcatggggc gaggtcgatc cagacgaact ggaccgcgag ctcgatgagc
    13561 atttgatcga gcagcccaac gggcgggcgg gatggcggat cagcatcccg gcgatgttgt
    13621 cgtactggag cgagctggcg cgcccaatta gcttgccccg cagtggaata ccggtcacgc
    13681 tcgtgattgc cacgaagacc gacccgccgt acgtgacgga tgaattgctc accggactcc
    13741 gggaacgccc cgacttcaca ttcgtcgagt tcgactgcga ccacatggtt ccgcaggcgc
    13801 ggcctgccga ggctgcggcg gtgatccgca agcagctgga gcgcggctga gatggccgcg
    13861 gtcaccgacg aacaggtcga gaccgtgcgc gcgctggtcg cgtcgatccc gtccggccgg
    13921 gtgtcgacct acggtgacat cgctgaggtg gcagggcttt ccagtccgcg cattgtcggc
    13981 tggatcatgc gtaccgattc gtcggatctg ccgtggcatc gggtgatcag ggcatccggc
    14041 cggcccgcgc cgcacctgac gacgcggcag ttggagctgc tacgggccga gggcgtgctg
    14101 gccagcgacg gccggatcaa cctggccgaa gtgcgccacg agttctgagc gcgaacagac
    14161 gcaaagtcgc acgatttggt cgcggtttat gcgattttgc gtctgctcgc gcttctagag
    14221 caccagccgg accaaggcgg ccgtccgggc cagacccggg aacgccgcgg ccgtcgaccg
    14281 cgcatgcagt gcgtgcacag cgagccggaa catcaacgcg cgcaacaaca tctgcggcca
    14341 ctccggcagc gggctccagc gctcgatcag cccgtcgtcg gcttcgcccc atgccagcgc
    14401 gtccacgacc accactccag ccgcccagga cgacggccgc cagtacggcg tgatgtcggt
    14461 gatgccgggt gcggcggtgc ccgcgaaaag cactgtgccg tagagatcgc cgtgcaccaa
    14521 ctggttgggg ctcttggtcg gtcggcgcag cgcggcaagc tggttgatca actcgatcga
    14581 gcgctgcccg tcgggcgtgc ccggcgacac ccgggctccc gccggcagcg agtgcagcgg
    14641 ccgctcctcc cacgccgccc ggtcggcggc gatgaacacg tcgacgtcgg accacggcgc
    14701 aaccggcggc tgggtcaaga aacggggccg ctccagtttg gccgtcgcct cgtgcagccg
    14761 caccgccgcc gacaccacct cgtcatggcg cggctcaggc gtacccgcga cgtatgtatc
    14821 ggcccgccag cctgccacca cgtaacggcc gtcggtcgag cgcaccggcc gggccagccg
    14881 cacaccgtcg acgaacaacg tctcgcgaat tttggccgac cacgcggcgc gggcgtggtc
    14941 ggcaaccatc gacagcacga cctcgccgca gcgccagccg ccctcccagc tcgacccgag
    15001 cgcaaccggc tgcgcgccgg tcagtccgaa cgccgccagc acatgatccg gcggccgttc
    15061 gacagtcacc acctcagcgt aagcgctgac tccgctaaca cggctcaacc atcgcgatac
    15121 cgcgaaatcg tggcggaacg tttggcgatg agacttgtcg gagtcgtgtg caactgtgct
    15181 tgtgagatga cgcggcatac caccttcaag tactgccttg ccccgacggc cgaacagcaa
    15241 tgtgttctag cacgccatgc gggggcggcg cggttcgcct acaaccagtc cctacgcatt
    15301 gtgaaggatg cgctggatgt ccgttgaacc gacgcggagg tggatgtgcc gtttaccgcc
    15361 attgacctca tcaatgcatt caacgcgtgg aagaagtcgg aagccgcggg aactgacgtt
    15421 catcccgaga gatcggcatg aaccgaagac gcccgagaag gccggtgccg gaccggtcgc
    15481 ccggcacgct ttaaccaggt gtcagtacat caccatgtcg ggctcgagtt gcttgcccca
    15541 cgcgacgatt ccgccctgca catgcatcgc gtcggagaag cctgccttct tgacgatggc
    15601 gagcacctcg gccgagcgca cgcccgtctt gcagtagaag accggggtgc ggtcgacctg
    15661 aagcttggcg agcgcctcgc ccgattcgaa cgcgcccttg gggatcagct ctgcgccctc
    15721 gatgcggttg atgtcccact cgaccggttc acgaacgtcg atcagcgcca acggcttacc
    15781 ggaatccaga agctccttca actcacgcgg cgtgatcgtg gagtcggcag cggcttcggc
    15841 ggccgcttcg gacaccacgc cgcagaactc ctcgtagtcg atcagctcgg tgatcttcgg
    15901 cgtggccggg tccttgcgga tcttgatggt gcggtaggtc atgtcgagcg cgtcgtagac
    15961 catcagccgg cccagcagcg gctcgccgat gcccgtgatc agcttgatcg cttcggtgcc
    16021 catcaccgag gcgatcgacg cgcacaggat gcccagcacg ccgccctcgg cgcacgacgg
    16081 caccatgccg ggtggcggcg gctccgggta caggtcgcgg taattcaggc cgagtccgtc
    16141 gggcgcgtcc tcccagaaca ccgacacctg gccctcgaag cggtagatcg acccccacac
    16201 atacggcttg ccggccagca cagcggcgtc gttcaccaga tagcgggtcg cgaagttgtc
    16261 ggtgccatcg aggatcaggt cgtactgctc gaacagctcg acggcgttgt ccggctccag
    16321 ccgaaactcg tgcagccgca cctcgacgag cgggttgatc tcgcgaaccg agtcccgtgc
    16381 gctctccgct ttggaccgac cgatgtcgga ctggccgtgg atgatctggc gctgcagatt
    16441 ggactcgtcg acaacatcga actcgacgat gccgatggtg cccacgcccg cggcggccaa
    16501 gtacaacagc gtcggcgagc cgagcccacc ggcaccgatg accaacacct tcgcgttctt
    16561 cagccgtttc tgcccgtcca ctcccaggtc cgggatgatg aggtgacggc tgtagcgcgc
    16621 gacttcctcg cgcgtcagct cgccggccgg ttctaccagc ggcggcaacg gagtcgacac
    16681 cgaaagtctc cttatttgac ggtcactgca gtacccacca caacggtagt ccggtcgcga
    16741 tgcttcccga cggcgagtcg gcgcgctagg cgatcgggta cggccagggg ttgaagcggc
    16801 aggtcttgcc gtcgggtttg accgactccg ggtcgaaccg ggcggcgtca ttgttggtgg
    16861 tcgagaacgt ctgctgcatc atgatcggcg ccagcccgcc gtcctgcccg catccttcgt
    16921 gtcgctgata gcccatcgcg tgcccgattt cgtgattgac caggtactgc cggtaggagc
    16981 cgacgtcccc ctggaacggg acggcgccgc ggacccagcg ggcctcgttg atgaacaccc
    17041 gaggctggtc ggcgtaggcg gggttgtagc aggaggcctc gagttggatg tcgtagccgc
    17101 agccctcccg cactgtcatc ggcgagctga gcgacacccg gaaatcgggt tcgccgctgt
    17161 cgatccgggt gaacgcaaac tgcgggttgt gggtccagct cttggggttg gccagggttt
    17221 cgctgaccat ccgggcgaag ccgtcgtcgc cgccgaatgt cgtggtgtcg atgccgtcct
    17281 cga
//
LOCUS       DASRJA010000679         2987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3473735,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000679 DASRJA010000000
VERSION     DASRJA010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3473735"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VFC01_30525"
     CDS             complement(<1..684)
                     /locus_tag="VFC01_30525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN83986.1"
                     /translation="MTTQISHFIDGKRTAGQSKRTADVMNPNTGEVQAKAPMASSADV
                     DAAVAGAVEAQKEWAAWNPQRRARVLMKFIDLVNKNVDELAETLSLEHGKTLADSKGD
                     IQRGIEVIEFAVGIPHLMKGEYTEGAGTGIDVYSMRQPLGVVAGITPFNFPAMIPLWK
                     AGPALACGNAFILKPSERDPSVPVRLAELFIEAGLPPGVFQVVHGDKEAVDAILEHPE
                     IQAVGFVGSS"
     gene            complement(715..1128)
                     /locus_tag="VFC01_30530"
     CDS             complement(715..1128)
                     /locus_tag="VFC01_30530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83987.1"
                     /translation="MILRAGMWFLTLVELAVGLVATLAPRVFYDYVPWVDLAPPFAEH
                     LMRDYGAMNLALGLVTAVAAITMDRVMVRTSLAAYLMFAVPHLLFHVTHHDHYTASQA
                     IGETTALTIAVLLPLALLVLSWAPCTPCRRDTRMS"
     gene            complement(1125..1931)
                     /locus_tag="VFC01_30535"
     CDS             complement(1125..1931)
                     /locus_tag="VFC01_30535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZN83988.1"
                     /translation="MTTRILVTGGTGTLGRVVVEHLQLAGADVRVLSRGRRPRSADVE
                     HVRGDVRSGGGLDSAVAGVDTIVACVDPVEQLVAAAKRAGSPHLVYISIVGVDQVPFR
                     YYRQKLADEQLIAASGLPWTVLRATQFHDLIAVVMRMLTKPPVMAIPAGVSDQPVDVR
                     DVGPRLARLALGEPAGRAPDFAGPQVRTFADLARSYLEMAGKRRPIMPVRFPGKVFRA
                     FRAGGHLAPGHAAGTITFEEYMADQLASGHLPYADAINHYLKPHRRKAPQ"
     gene            complement(1936..2841)
                     /locus_tag="VFC01_30540"
     CDS             complement(1936..2841)
                     /locus_tag="VFC01_30540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HZN83989.1"
                     /translation="MTDFAAEFETHRSQLFGLAYRLLGSASEAEDAVQNTYLRLHSAD
                     RNAIRSLPAWLTKALTNLCLNELTSARARRETYTGPWLPEAVFTADGALGPLETVEQR
                     DAVSIAMLALMERLTPAERAAFVLREAFAYSHADIADILDTSEANARQLHRRARQRLD
                     QRVPRFRPDPAHWRALVERFLDAARGGDVPGLAAMLADNVSSTADGGGQVGAARRVVT
                     GRDRVARYLAGAFSRPIADVRLDFGAEINGEPAVLGFVGSTLAGVLFFEITDDRVVAL
                     RIVANPDKLRFLGTQLSHPVGLPGS"
BASE COUNT          498 a          982 c         1027 g          480 t
ORIGIN      
        1 ggagctgccg acgaaaccga cggcctggat ctcggggtgt tcgaggatcg cgtcgacggc
       61 ctccttgtcg ccgtgcacga cctggaacac accgggcggc aggccggcct cgatgaacaa
      121 ctcggccagc cggaccggca ccgaagggtc acgctcgctg ggcttgagga tgaacgcgtt
      181 gccacacgcc agcgcgggcc cggccttcca cagcgggatc atcgccggga agttgaacgg
      241 cgtgatgccg gcgaccacgc ccagcggctg gcgcatcgag tagacgtcga tacccgtgcc
      301 tgcgccctcg gtgtattcgc ccttcatcag gtggggaatg ccgaccgcga actcgatcac
      361 ctcgatgccg cgctggatgt cgcccttgga gtcggcgagg gtcttgccgt gctcgagcga
      421 cagtgtttcg gcgagctcgt cgacgttctt gttgaccagg tcgatgaact tcatcaacac
      481 gcgggcgcgg cgctgcgggt tccacgccgc ccactccttc tgcgcctcga ctgcgccggc
      541 gactgcggcg tcgacgtcgg ccgacgacgc catcggcgcc ttggcctgca cctcgccggt
      601 gttggggttc atcacgtcgg cggtgcgctt ggactgtccg gcggtgcgct tgccgtcgat
      661 gaaatgcgaa atctgtgtgg tcatgagtct gtccctcgcc cgaatggctg aatactagga
      721 catcctagta tctctccggc agggtgtgca aggggcccaa ctgagcacca acagcgcgag
      781 cggaagcagc accgcgatcg tcagcgccgt cgtttcgccg atggcctgcg aggccgtgta
      841 gtggtcgtgg tgggtgacgt ggaacagcag gtgcggcacg gcgaacatca ggtacgcggc
      901 cagcgaggta cgcaccatga cgcgatccat cgtgattgcg gcgacggcag tgaccaatcc
      961 cagtgcgagg ttcatggcgc cgtagtcgcg catcaggtgt tcggcgaacg gcggcgccag
     1021 gtccacccag gggacatagt cgtagaacac ccgcggcgcc agcgtcgcca ccaaccccac
     1081 cgccagctcg accaaggtga ggaaccacat cccggcccgc aggatcattg cggcgccttc
     1141 cgtcggtgag gtttgaggta gtggttgatg gcgtcggcgt agggcaggtg tcccgaggcg
     1201 agctgatcgg ccatgtactc ctcgaacgtg atcgtgcccg cggcgtgccc cggcgccagg
     1261 tgcccgccgg cgcggaaggc gcgaaacacc ttgccgggga accgcaccgg catgatcggc
     1321 cgtcgcttgc cggccatctc gaggtagctg cgcgcgagat ccgcgaaggt gcgaacctgc
     1381 gggccggcga aatcaggtgc gcgtccggcc ggttcaccga gggcaagacg ggccagtcgc
     1441 gggccgacgt cgcgcacatc gaccggttgg tcgctcactc ccgcgggtat cgccatcacc
     1501 ggcggtttgg tcagcattcg catgacgacc gcgatcaggt cgtgaaattg cgtggcgcgc
     1561 aatacagtcc acggcaggcc ggacgcggcg atcagttgct cgtcggcgag cttctgccgg
     1621 tagtaccgga acggcacctg gtcgacgccg acgatcgaga tgtacaccag atgcggcgag
     1681 cctgcccgct tggcggcagc gaccagctgc tctacggggt ccacgcacgc gacaatggtg
     1741 tcgacgcccg cgacggctga atccagcccg ccgccactac gcacgtcacc cctgacatgt
     1801 tcgacatcgg cgctgcgggg ccgccgtccc cggctcagca cgcgcacatc ggcaccggcg
     1861 agctgcaggt gctccaccac gacgcggccg agcgtgccgg tgccgccggt gaccaggatt
     1921 cgggtcgtca catagttatg aaccgggcag ccccaccgga tgtgacagct gcgtgccaag
     1981 gaatcgaagt ttgtcgggat tggcgacgat ccgcagcgcc accacccggt cgtcggtgat
     2041 ctcgaagaac agcacaccgg ccaatgtcga gccgacgaat ccgagcaccg ccggctcgcc
     2101 gttgatctcc gcgccgaagt cgagccgcac gtcagcgatc ggtctgctga acgcgcccgc
     2161 cagataacgg gccacccgat cgcgcccggt cacaactcgg cgcgcggcac ccacctgacc
     2221 gccgccgtcg gcggtggagc tgacgttgtc ggccagcatc gccgccaggc cggggacatc
     2281 accgccgcga gcggcatcca aaaagcgttc gacgagcgcg cgccagtgtg cgggatccgg
     2341 ccggaaccgc ggcactcgtt ggtccagtcg ctgtcgtgcg cgtcggtgca actggcgcgc
     2401 gttggcctcc gacgtgtcga ggatgtctgc gatgtcggcg tggctgtagg cgaatgcctc
     2461 gcgcaagaca aacgcggcgc gctcagcggg ggtaagccgc tccatcagtg cgagcatcgc
     2521 gatggatacg gcgtcgcgct gctcgacggt ttctagcggg ccgagtgcgc cgtcggcggt
     2581 gaagacggcc tcgggaagcc aagggccggt ataggtttcg cgacgcgcgc gggccgaggt
     2641 gagttcgttg aggcacaggt tcgtcagcgc cttggtcagc cacgcgggca gtgaccggat
     2701 tgcattccgg tcggcggagt gcaggcgcag atacgtgttc tgtaccgcgt cctcggcttc
     2761 gctcgccgag cccagcagcc ggtacgccag cccgaacagc tgcgagcgat gcgtttcgaa
     2821 ttccgcggcg aagtcggtca ctgagtcaag agtgccacgc cgcgctggtg aggacgtgac
     2881 gctcaaggaa ttccagttga tcggcgacga ccgcctcatg ctgcggcccg tcgtagatgt
     2941 cgaagtgatc tacgccggga tacaaccgca gctctccgcg tggcgcg
//
LOCUS       DASRJA010000680        10009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3778865,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000680 DASRJA010000000
VERSION     DASRJA010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10009
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3778865"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VFC01_30545"
     CDS             <1..687
                     /locus_tag="VFC01_30545"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZN83990.1"
                     /translation="SSYKLLIGSILPRAIAWVSTQSTTGVGNVAPVSFFTVVGRFPPV
                     LSITLQPRSDGVTLKDTFVNIRDTNEFVVNIVSIGLADAVHRSAYEFDSDVDEFEALG
                     LKKAASEMISAPRIGEAPISFECVVDRIIPMPPLPDHVVWGRVKRIHIRDDLYLPNGR
                     IDVGALGVFGRLAGEYTMVDNIFTTPLPEELVEELTALRASRLDGRATDYSPIDTAEW
                     SPSGSTKAAS"
     gene            687..1481
                     /locus_tag="VFC01_30550"
     CDS             687..1481
                     /locus_tag="VFC01_30550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN83991.1"
                     /translation="MTQSTNGFRVTRTPADTAGAPTVLLIHGFLDDATVWDGLVECLA
                     GEVRAVRYDLPGFGTRSGSVADASVVTLESLAAEAGDIVAGIDGPVIVVGQSLGTQIA
                     ELVAAQHPDHVHGLVLLTPVPLGGTQLPDEVVAPFRALGGDRVAQRSLRAELSPHLDE
                     KQLDWLTDIAAPVAREVTDHYVDVWNNGIGDAPPTSAYRGPVMIIRGGADGFATEQLV
                     DAISPRFVQPELKVIDKGGHWVHAEYPGTVATMILDFIDAIEEVAA"
     gene            1478..2329
                     /locus_tag="VFC01_30555"
     CDS             1478..2329
                     /locus_tag="VFC01_30555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN83992.1"
                     /translation="MNRKLRYRTFVTKQIPQSGRGPLPDGKTRMWSPITSTLILGRHD
                     AVLIDPPLTTTQAAEVGDWIAASGRQLRQVYITHGHGDHWFGAIPLLQRFPGVAVQAT
                     SGTAKLMEAQNDPGFRAEFWDRVFPGQLPGGELDVSIVDEHGFEFDGVALIPVEVGHT
                     DTDATTMLHVPDMGLLVAGDVVYNGVHLYLTESGGVAGIDEWLAALDTAERLRAATVI
                     AGHKDPRAFDNPSQIQATRRYLTDARRLLESSETAEEFYREMLRLHPGRINPGALWGA
                     AITLLPS"
     gene            complement(2366..3085)
                     /locus_tag="VFC01_30560"
     CDS             complement(2366..3085)
                     /locus_tag="VFC01_30560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN83993.1"
                     /translation="MADLLSAVLHAHGGLDRWRQLIRVEATIVTGGGLWAIKGQPQDP
                     LPRRMSAALHHEWASVQPFGGDDQKTDFTPERVAIEKLDGRVVSERAGPRASFDGHEF
                     STPWDPLHRAYFNGYALWTYLTTPFLLTLDGVSVHEIASVMEDGQTWRGLQAEFPPEI
                     ATHSSVQEFYFGDDDLLRRHDYRVDVAGGFPAVQYVYDIVEADGIKLPSKRRAYRADQ
                     HGNAIPDQLMVAIDLSDISFH"
     gene            complement(3133..3759)
                     /locus_tag="VFC01_30565"
     CDS             complement(3133..3759)
                     /locus_tag="VFC01_30565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZN83994.1"
                     /translation="MHIAILTFEGYNELDSLIALGVLNRVRRTDWRVSIASPSPRVRS
                     MNGVVIESHVSLGEASVLDAVIVGSGIRTREVVADQALMAQLRLDPSRQLVGAQCSGT
                     LVLAKLGLLDGVPACTDSTSKPWVHEAGVEVLDQPLFAKGNMATAGGCLSSAYLAGWM
                     IAHLDGVDAAADALHYVAPVGEKEEYVECAMRNITPYLPENRRARLRV"
     gene            3822..5156
                     /locus_tag="VFC01_30570"
     CDS             3822..5156
                     /locus_tag="VFC01_30570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZN83995.1"
                     /translation="MVQARYKEVVDALAADIRSGKIPPGTRLPTHRELASREGLAVVT
                     ATRVYAELAAMGLVSGEAGRGTFVREAALSGRDDIDQRAFADDVVDLNFNYPSIPRQA
                     DLLRNALRGLASSGDLEALLRYQSHGGRPHERASAALHLEQRGLTVPADQVLIVSGAQ
                     HGLAITAMAMLRPGDVIAVDALTYPGFKVLAQIHGLELAPLPARVDGPDLDALAQLCM
                     TRRVRAVYTMPTLHNPLGWVSSEQSRKKLVEIARKHGLLIIEDAAYAYLVEEPPTPVA
                     ALAPESTIYVSGLSKNVATGLRFGFVAAPMQYIEGLTRAIRATTWNTPALVTAIATGW
                     LNDGTVDRLEAEKRADARHRQAVAREVLSGLDLVGHPASYFLWLPLPEDARADRVAMA
                     LMRENISISTAEPFATTKSPPHALRLALGSVPLDVLAQSLNQVKQAIDAYTY"
     gene            complement(5188..6018)
                     /locus_tag="VFC01_30575"
     CDS             complement(5188..6018)
                     /locus_tag="VFC01_30575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019931860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN83996.1"
                     /translation="MTGERSYPQGVPCWVDTEQSDVDAARDFYANLFGWTFADAIPAD
                     ATGRYLIASLDGKDVAAIGPAQGDAPVEWTTYIAVEDADAAADSVRATGGRVTLEPVD
                     AGPGGRLAACADPQGARFRLWQPRRRLGAQLVNAPGSWNFSDLHTGDAAAATKFYSPL
                     FGWEFDDIGFATMIRRPGYGDHLAATVDPGIRERQEGISAPPGFADAIAWLAPLASGD
                     DHWHVSFTVASRDESAATAEELGAVVISSDDTEWTKSALIRDPQGAELTLSQFTPPNP
                     "
     gene            6336..6806
                     /locus_tag="VFC01_30580"
     CDS             6336..6806
                     /locus_tag="VFC01_30580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HZN83997.1"
                     /translation="MTTTTPKYMAVQGAALLVATGFLVIGILGFIPGVTTDYGLLDWT
                     GHHSGAKLFGVFAVAGVHNVMHLAFGVVGMILTRSYAAARAYLIGGGLAYLALWVIGL
                     FIDGSDLVPLNGASNLLHYAIGGVMVILGLTLGGQRDPTKRRQRRRSPKSAPGL"
     gene            complement(6820..7758)
                     /locus_tag="VFC01_30585"
     CDS             complement(6820..7758)
                     /locus_tag="VFC01_30585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HZN83998.1"
                     /translation="MTRVAAIDCGTNSIRLLIADKVDGRLQDVHREMRIVRLGQGVDA
                     TGQFAPDALARTKAALVDYAELLRSFEVPKVRMVATSAARDAANRDVFFAMTAEVLGA
                     VVPGAVAEVITGTEEAALSFHGAVDELDPDSAPFVVVDLGGGSTEVVLGSRDVDASFS
                     ADIGCVRLTERCLHSDPPTAAEIAAAREAVRDGLGEALRVVPVERARTWVGVAGTMTT
                     LSALAQKMTTYDSEAIHRSRVGFGDLLQVCDELIAMTRQQRAALGPMHEGRVDVIGGG
                     AIIVEELASVLGHRAGIDELVVSEHDILDGIALSIA"
     gene            complement(7755..8246)
                     /locus_tag="VFC01_30590"
     CDS             complement(7755..8246)
                     /locus_tag="VFC01_30590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HZN83999.1"
                     /translation="MVDAADLDAVARQLGREPRGVLEIAYWCPNGEPGVVKTAPKLPD
                     GTPFPTLYYLTHPVLTAAASRLESSGLMREMTERLQQDPELAAAYRRAHESYLAERDA
                     IESLGTTFSGGGMPDRVKCLHVLIAHSLAKGPGVNPLGDEALAVLADEPAMAGILEKG
                     KWT"
     gene            complement(8239..8886)
                     /locus_tag="VFC01_30595"
     CDS             complement(8239..8886)
                     /locus_tag="VFC01_30595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HZN84000.1"
                     /translation="MPEAKRPDPKRRSPAGRPKGTSPARRQPRATESKSTLEAEPKEK
                     PKETIRESIAASAEQQSEQRFGSAARRAAILAAVICVLTLTIAGPVRTYFAQRTEMKQ
                     LEATEKQLRAQIAGLEEQKVKLADPVFVAAQARERLGFVMPGEIPYQVQLPANAAVPG
                     TPGGGPAATKSGDPWYTSLWHTIADAPHGVSPTIAPDAPPGPLPLPPAAPSAPGG"
     gene            complement(8890..>10009)
                     /gene="eno"
                     /locus_tag="VFC01_30600"
     CDS             complement(8890..>10009)
                     /gene="eno"
                     /locus_tag="VFC01_30600"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZN84001.1"
                     /translation="YGGKGVEQAVQGVLNEIAPELVGLDATDQRTIDQRLLDLDGTPD
                     KSRLGANAMLGVSLAVAKGAAESAGLPLFRYIGGPNAHILPVPMMNILNGGAHADTGV
                     DVQEFMVAPIGAPSFKESLRWGAEVYHSLKSVLKKQGLATGLGDEGGFAPDVAGTKAA
                     LDLILSAVEATGLKVGSDVALALDVAATEFFTDGKGYTFEKETRTAEQMSDFYAGLID
                     SYPLVSIEDPLSEDDWDGWVALTTAIGDRVQIVGDDLFVTNPERLEDGIEKGAANALL
                     VKVNQIGTLTETLDAVALAHSSGYRTMMSHRSGETEDTTIADLAVAVGSGQIKTGAPA
                     RSERVAKYNQLLRIEETLGDAARYAGDLAFPRFSVEAK"
BASE COUNT         1705 a         3380 c         3154 g         1770 t
ORIGIN      
        1 tcgagctaca aacttctcat cggcagcata ttgccgcgcg cgatcgcctg ggtcagcacc
       61 cagtcgacca ccggagtggg caatgtcgcg ccggtgtcct tcttcactgt ggtcggacgc
      121 ttcccgccgg tgctctcgat cacgttgcag ccgcgctccg acggggtgac tctcaaagac
      181 acgttcgtca acatccgcga caccaacgag ttcgtcgtca acatcgtctc gatcggcctg
      241 gcagacgccg tgcaccgctc tgcctacgaa ttcgatagcg acgtagacga gttcgaggcc
      301 ctcggcctga aaaaggccgc gtccgagatg atctcggcac cccgcatcgg cgaggccccg
      361 atctccttcg aatgtgtggt cgaccgcatc atcccgatgc cgccgctgcc tgaccatgtc
      421 gtgtggggac gagtcaagcg catccacatc cgcgacgacc tctacctgcc caacggtcgt
      481 atcgacgtgg gcgcactggg ggtattcggg cggctcgcgg gcgagtacac catggtcgac
      541 aacatcttca ccacgccact gcccgaagaa ctcgtcgaag agctcaccgc gctgcgcgcg
      601 agccgcctcg atgggcgcgc cactgactac tcgccgatcg acacggctga atggtcaccg
      661 tcgggctcga cgaaggcggc gtcataatga cccagtcaac caacgggttt cgcgtcaccc
      721 gcaccccggc agacacggcc ggcgcgccca cggtgctgtt gatccacggc ttcctcgacg
      781 atgcgacggt gtgggacggt ctggtcgaat gcctggccgg tgaggtccgt gccgtgcgct
      841 acgacctacc cggattcggc acgcgaagcg ggtccgtcgc cgatgcgtcc gtcgtcacgc
      901 tggaatcgct tgcggccgag gccggcgaca tcgtcgcggg aatcgatggt ccggtgatcg
      961 tggtcggcca aagcctcggc acgcagatcg ccgagctggt ggccgcgcag caccccgacc
     1021 acgttcacgg gctggtgctg ctcactcccg tcccgctcgg cggcacgcag ctccccgacg
     1081 aggtggtcgc cccgttccgg gcgcttgggg gtgaccgcgt tgctcagcgc agcctgcgcg
     1141 ccgaactgtc gcctcacctc gacgaaaaac agctggactg gctgaccgac atcgccgccc
     1201 cagtcgctag agaagtcacg gaccactatg tcgacgtctg gaacaacggg atcggcgatg
     1261 cacctccgac gagtgcatac cgcggtccgg tgatgataat ccgcggcggc gccgacggtt
     1321 tcgccaccga acagctcgtc gatgccatca gcccgcgctt cgtccaaccc gaactcaagg
     1381 tcatcgacaa gggcggccac tgggtgcatg ccgagtaccc cgggaccgtc gcgacgatga
     1441 tcctcgactt cattgatgcg atcgaagagg tggcggcgtg aaccggaagt tacgttacag
     1501 aacctttgtc acaaaacaga ttccgcaatc gggccgaggg ccgctgcccg atggcaagac
     1561 ccgaatgtgg tcgccgatca cctcgacgct gatcctggga cgacacgacg cagtgctcat
     1621 cgacccgccc ctcaccacaa ctcaagcggc agaagtcggc gactggatcg cggcgtcggg
     1681 acgccaactg cgtcaggttt atatcaccca cggccacggg gaccattggt tcggcgcaat
     1741 tcctctgctg caacgattcc cgggagtcgc cgtgcaagcg acgagcggaa ccgccaaact
     1801 gatggaggcg cagaacgatc caggatttcg cgccgagttc tgggatcggg tgttccccgg
     1861 acaactaccc ggtggagaac tggacgtctc catcgtcgac gagcacggct tcgaattcga
     1921 tggcgtcgcg ctgattccgg tcgaggtcgg tcacaccgat accgacgcga cgacgatgtt
     1981 gcatgtaccc gacatgggtc tgctcgttgc aggtgacgtc gtctacaacg gtgtgcatct
     2041 gtacctgacc gaatctggtg gtgtcgccgg catcgacgag tggctagcgg ccctcgacac
     2101 cgccgaacga ttacgcgccg caacggtgat cgccggtcac aaagatccac gcgcctttga
     2161 caacccatca cagatccagg cgacacggcg ctatctcacc gacgcgcgcc gactgctcga
     2221 gtcgtcggaa acagccgagg agttctaccg ggagatgctg cgtctgcacc ctggtcgaat
     2281 caaccccgga gcgctgtggg gggcggccat cacgctgctg ccgtcgtgac agaggcgctc
     2341 ttgggtggca ggcagtgagc accgatcaat gaaagctgat gtcgctcaga tcgattgcga
     2401 ccatcagctg atccggaatc gcgttgccgt gttggtcggc acggtaggcc ctgcgtttcg
     2461 acggcagttt gatgccgtcc gcctcaacga tgtcgtagac gtattgaacg gcaggaaagc
     2521 ctccggccac gtcgacgcga tagtcgtggc ggcgcagcag atcgtcgtct ccgaaataga
     2581 actcctgcac cgaactgtgc gtggcgatct caggtggaaa ctccgcctgc aggccgcgcc
     2641 acgtttggcc gtcctccatc accgaagcga tctcgtgcac agatacgccg tccagcgtca
     2701 gcaggaacgg cgtggtgaga taggtccaca gcgcgtagcc gttgaagtag gcgcggtgca
     2761 gcggatccca cggcgtagag aattcatgcc cgtcgaacga cgcgcgaggg ccagcccgtt
     2821 cggaaactac tctgccgtcg agcttttcga tcgctacacg ttcaggagtg aagtcggtct
     2881 tctggtcatc gccgccgaac ggttgtaccg aagcccattc gtggtgcaac gccgcggaca
     2941 ttcgccttgg cagcggatct tggggctggc ctttgatcgc ccacaggcct cctccggtga
     3001 cgatcgtcgc ttcaacgcgg atgagttggc gccaacggtc aagcccgccg tgcgcgtgga
     3061 gtactgcgct gagcaagtcg gccatgcgaa ccctttctgt cgctaacggg cccgagcgcg
     3121 agtacgtcga cgctacaccc gaaggcgggc ccgccggttt tcgggcaagt agggcgtgat
     3181 attccgcatt gcgcattcga cgtactcctc cttctccccg accggcgcga cgtagtgcag
     3241 ggcatctgcc gccgcgtcga caccgtccag gtgtgcgatc atccaccccg cgagatacgc
     3301 cgaggacagg cagccgcccg ccgtggccat attgcctttg gcgaaaagtg gttgatccag
     3361 cacctcaacg ccggcctcat gaacccacgg tttggacgtc gagtcggtgc aggctggaac
     3421 tccgtcaagc agcccgagct tggccagtac cagcgtgccg gaacactgcg cgccaaccag
     3481 ttggcgcgat gggtcgagcc gcaactgcgc catcagggct tgatccgcga cgacttcgcg
     3541 ggtccgaatg ccgctgccca cgatcaccgc atccaagacg ctggcttcgc caagcgagac
     3601 atgtgactcg atgaccacgc cattcatcga gcggactcgt ggactggggc tggcgatcga
     3661 gacccgccag tctgtcctgc gaacccggtt gagaacgccg agtgcgatca gtgagtcgag
     3721 ttcgttgtag ccctcgaacg tgaggatggc gatgtgcatg gcccgatcgt atgaccgcca
     3781 acccagtacg atcaaagaat tgtcatgcat acaatcattg agtggtccag gcccgatata
     3841 aagaagtcgt cgacgcgctg gccgccgaca tccgctccgg caagatcccg ccaggtacgc
     3901 ggttgccgac tcatcgcgag cttgcatccc gtgaagggct ggcggtggta accgcgaccc
     3961 gggtctacgc ggagctcgcg gcgatgggcc ttgtcagtgg cgaagccggt cgaggcacct
     4021 ttgtccggga ggcggccctg tcgggtcgcg acgacatcga ccagcgcgcg ttcgccgatg
     4081 acgtcgttga cctcaatttc aactatccgt ccataccccg acaggccgac ttgctccgaa
     4141 atgcgttgcg agggcttgcc tcctccggag atttggaggc cctgctgcgt taccagtctc
     4201 acggcggccg gcctcacgag cgagcctcgg ctgcgctcca cctcgaacag cggggactta
     4261 cggtgcctgc ggatcaggtg ttgatcgtca gcggtgcgca gcacggattg gccatcacag
     4321 cgatggcgat gctgcgaccc ggcgacgtga ttgcggtcga cgctttgaca tatccggggt
     4381 tcaaggtgct cgcccaaata cacggcttgg agttggcgcc gctacccgca cgtgtggacg
     4441 gtcctgatct cgacgcgctc gcccagctgt gcatgacgcg gcgagtgcga gcggtctaca
     4501 cgatgccgac tctgcacaac ccgctcgggt gggtgtcctc cgagcagtcc cgtaagaagt
     4561 tggtggagat tgcccgaaaa cacggacttc tgatcatcga ggacgccgcg tacgcgtact
     4621 tggtggaaga gccacctact ccggtggcag cgcttgcgcc ggagtcgaca atctacgtct
     4681 ccgggctgtc caagaacgtg gctaccggcc ttcgcttcgg gtttgtagct gcgccaatgc
     4741 aatacatcga aggcctcacc cgcgccatcc gcgcaacgac ctggaacaca cccgcgctgg
     4801 tgaccgcgat cgcgaccgga tggctcaatg acggcaccgt tgatcgccta gaagcagaga
     4861 agcgtgccga cgcgcggcac cgccaagcgg tggcccgcga ggtgctcagt gggctcgacc
     4921 tcgtcgggca tccagcctcc tatttcctgt ggctgccctt gccggaggac gcccgagccg
     4981 accgggtggc tatggcgctt atgcgcgaga acatctcgat atccacggcg gaacctttcg
     5041 cgacgaccaa gagcccacca cacgccctca ggttggcgct cgggtcggta ccgctagacg
     5101 tcctggctca gtcactaaac caagtgaaac aggcgatcga tgcctacacg tattgagcga
     5161 cagcgtgttg ggcgaacagt gacgccgtca cgggttcggc ggagtgaact ggctcagcgt
     5221 cagctccgcg ccctgcggat cgcgaatcag cgcggacttc gtccactcgg tatcgtccga
     5281 cgaaatcacc acggcgccaa gctcttcggc cgtcgccgcc gattcgtcgc gtgacgcgac
     5341 cgtgaagctg acgtgccagt gatcatcgcc actcgccagc ggcgccaacc atgcgatggc
     5401 gtccgcgaag cccggcgggg cggagatgcc ctcctgccgc tcccggattc cggggtcgac
     5461 ggtcgcggcg agatggtcac cataaccggg tcgcctgatc atcgtcgcga agccgatatc
     5521 gtcgaattcc caaccgaata gcggcgagta gaacttggta gctgccgccg cgtcgccggt
     5581 gtgcaggtcg ctgaagttcc agctgccggg ggcgttgacg agttgcgcgc ccagccgccg
     5641 tcgcggctgc cacagccgga atcgcgcgcc ctgcgggtcg gcgcaggccg ccagccgacc
     5701 tcccggcccg gcgtcgacgg gctcgagcgt gacgcggcca ccggtcgcgc gcacggaatc
     5761 cgccgccgcg tcggcgtcct cgaccgcgat atacgtcgtc cactcaaccg gcgcatcgcc
     5821 ctgcgccggc ccgatcgccg ccacgtcttt gccgtcgagc gacgcgatca agtacctccc
     5881 cgtcgcgtcg gctggtatcg cgtcggcaaa cgtccacccg aacaggttcg cgtagaagtc
     5941 acgggcggcg tccacgtcgg attgttcggt gtcgacccag cacggaactc cttggggata
     6001 gctgcgctca ccggtcattg gcttcacttt cctttatttc caaagtgatg cgaactattg
     6061 ttatgtcgtc atatgttgat atgcgtccgt ggttacaaac tctgctccca ccctctcagg
     6121 tggtcagagc aacagaggag ttgatcgacg atgcccgtgt cacagatcaa ggccggttcc
     6181 ggctgtagct gtcattgcaa tgcgtgcgag ggtggccatc actgcggcaa cccgcccaac
     6241 tgcaacatca gaaggtagct aactcgaaca tctccgatta gcccggcgtc gcccggggta
     6301 tttctcctgc gtaaataagc gcgggaggtg aggcgatgac gacgacaacc cccaagtaca
     6361 tggcggtgca gggggcggcg ctgctggttg cgactggctt cctggtcatc gggatcctcg
     6421 gcttcatccc gggtgtgacg accgactacg ggctgctgga ctggaccggg caccattccg
     6481 gcgcgaagct gttcggggtg ttcgccgtgg cgggtgtgca caatgtcatg cacctggcgt
     6541 tcggcgtcgt cgggatgatc ttgacccgca gctatgccgc ggcgcgggcc tacttgatcg
     6601 gcggcgggct cgcctacctc gccctgtggg tcatcggatt gttcatcgac ggctccgatc
     6661 tcgttccgct caacggtgcc agcaacttgc ttcactacgc gatcggcggg gtcatggtga
     6721 tcctcggcct cacccttggc ggtcagcgcg acccgaccaa gcgtcgtcaa cgccgccgtt
     6781 cgccgaaatc tgcaccaggg ctgtaaatct cgcttgccgc tacgcgatcg acagcgcgat
     6841 gccgtccagg atgtcgtgct cactgaccac gagctcgtcg atgcccgccc ggtggcccaa
     6901 aacggatgcc agctcctcga cgatgatcgc gccgccgccg atgacgtcca cgcggccctc
     6961 gtgcatcggt cccagcgcgg cgcgctgctg acgggtcatc gcgatcaact cgtcgcacac
     7021 ctgcagcagg tcaccgaacc ccaccctgga ccggtggatc gcctccgagt cgtaggtcgt
     7081 catcttctgt gccagcgccg acagcgtggt catggtgccc gccaccccaa cccaggtgcg
     7141 ggcccgctcg acaggcacca ctcgcagcgc ctcgcccagc ccatcgcgga ccgcttcgcg
     7201 cgcggcggcg atctccgcgg ccgtcggtgg atcggagtgc aggcaacgct cggtcagccg
     7261 cacacatccg atgtcggcgg agaaactcgc gtcgacgtcg cgcgagccga gcaccacctc
     7321 cgtggaccca ccgccgaggt cgaccacgac gaaaggtgct gagtcagggt ccaattcgtc
     7381 gaccgcaccg tggaacgaca acgcggcctc ctcggtgccg gtgatcacct cggcgacagc
     7441 acctgggacc actgcgccca aaacctcggc ggtcatcgcg aagaacacgt cgcggttggc
     7501 ggcgtcgcgg gcggccgacg tcgccaccat ccgcaccttg ggcacctcaa agctgcgcag
     7561 cagttcggcg taatcgacca gcgccgcctt ggtccgcgcc aacgcgtccg gcgcgaattg
     7621 ccctgtcgcg tcgacgcctt gacccaaccg cacgatccgc atctcacgat gcacgtcctg
     7681 caatcggccg tcgaccttgt cggcgatcaa gagccggatc gagttggtgc cgcagtcgat
     7741 cgccgccacg cgcgtcaagt ccacttccct ttctccagga ttccggccat tgccggctcg
     7801 tccgccaaca ccgccagcgc ttcgtcgcca agcggattca cgcctggacc cttggccagc
     7861 gagtgcgcga tcagcacatg cagacacttc acccggtcgg gcatgccacc tccggagaac
     7921 gtcgtgccga gtgactcgat cgcatcacgc tcggccaggt acgactcgtg cgcccgccga
     7981 taagcggcgg ccagttccgg atcctgttgc agccgttcgg tcatctcccg catcagaccc
     8041 gacgactcca gtcggctggc cgcggccgtc agcaccgggt gcgtcaggta gtacagcgtc
     8101 ggaaacggcg ttccgtcagg cagtttcggc gcggtcttga ccactccggg ctcgccgttc
     8161 ggacaccagt acgcgatctc gagcacgccg cggggctcgc ggcccagttg ccgcgcgacg
     8221 gcgtcaagat ccgctgcgtc aaccaccggg tgcactgggc gcggcaggcg gcagtggaag
     8281 tggtccaggt ggcgcgtcgg gtgcgatggt cggcgaaaca ccatgcggcg catcggcgat
     8341 ggtgtgccat agcgacgtgt accacgggtc gccgctcttc gtcgccgcgg gcccgccacc
     8401 gggcgtaccg ggcacggccg cgttcgccgg cagctgcacc tggtacggaa tctctcctgg
     8461 catcacgaat ccgagccgtt cgcgcgcctg cgccgcaacg aacaccggat cggcgagttt
     8521 gaccttttgc tcttcgaggc ctgcgatctg cgcgcggagt tgcttttcgg tggcctcgag
     8581 ctgtttcatc tcggtgcgct gcgcgaaata ggtccgcacc ggtcccgcga tggtcagggt
     8641 gagcacacag atcaccgcgg cgaggatcgc cgcgcgcctg gccgccgacc cgaaccgttg
     8701 ctcggactgc tgctcggccg acgcggcaat ggattcgcgg atggtctcct tcggcttctc
     8761 cttgggctca gcctccaagg tcgactttga ctcagtcgcg cgcggctgac ggcgggcagg
     8821 cgacgtcccc ttcgggcggc ccgcagggga ccgtcgcttc gggtcgggcc gtttcgcctc
     8881 gggcacgggt tatttggcct ccaccgagaa ccgcgggaag gccagatcac cggcatagcg
     8941 ggcggcgtcg ccgagggtct cctcgatgcg cagcagctgg ttgtacttgg caacccgctc
     9001 gctgcgcgcc ggcgcaccgg tcttgatctg gccgctcccg accgcgacgg cgaggtcggc
     9061 gatcgtggtg tcctcggtct cgccgctgcg gtggctcatc atcgtccggt agccgctcga
     9121 gtgcgccagc gccaccgcat ccagcgtctc ggtgagcgtc ccgatctggt tgaccttcac
     9181 caaaagcgcg ttggccgcgc ccttttcgat gccgtcttcg aggcgctcag ggttggtgac
     9241 gaacaggtcg tcgccgacga tttggacgcg gtcgccgatc gcggtggtca gcgccaccca
     9301 gccgtcccag tcgtcctctg acagcgggtc ttcgatggac accagcggat acgagtcgat
     9361 cagtccggcg tagaagtccg acatctgctc ggcggtgcgg gtctccttct cgaaggtgta
     9421 accctttccg tcggtgaaga attcggtcgc ggcgacgtcg agcgccagcg ctacgtcgga
     9481 acccaccttc aagcccgtcg cctcgacggc actcaggatc agatcgagcg ccgccttggt
     9541 gcccgccacg tcaggcgcga agccgccctc atcgccgagg ccggtggcca gcccctgctt
     9601 cttgagcacc gacttcagcg agtggtagac ctcagcgccc cagcgcaacg actccttgaa
     9661 gctcggcgcg ccgatcggcg cgaccatgaa ctcctgcacg tcgaccccgg tgtcggcgtg
     9721 tgcgccgccg ttgaggatgt tcatcatcgg caccggcagg atgtgcgcgt tgggcccgcc
     9781 gatgtagcgg aacagcggca gccccgccga ctccgccgcc cccttggcca ccgcgagcga
     9841 cacgccgagc atggcgttgg cgcccagccg cgacttgtcc ggggtgccgt cgagatcgag
     9901 cagccgctgg tcgatcgttc gctggtcggt ggcgtccagc ccgaccagct ccggggcgat
     9961 ctcgttgaga acgccttgca cggcctgctc gacgcccttg cccccgtac
//
LOCUS       DASRJA010000681         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4083607,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000681 DASRJA010000000
VERSION     DASRJA010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4083607"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFC01_30605"
     CDS             <1..389
                     /locus_tag="VFC01_30605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN84002.1"
                     /translation="RSVSEEFERMGDLIAAADAAAHAAMTYRRQGKRGSALACSTSAD
                     ALAVRCGGACTPALRRASEALPLTDREAEIVMLMSEGLSYRAVAERLTLSVRTVESHM
                     YRAMLKTGTATRDELVALLPRHDALQ"
     gene            809..>2776
                     /locus_tag="VFC01_30610"
     CDS             809..>2776
                     /locus_tag="VFC01_30610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HZN84003.1"
                     /translation="MAVELSEHKRAHQPNEPIAILGIGCRLPGGVSSPDDLWQLLSEE
                     RDAVSEFPTDRGWNLDVLFGPDPDAPGATYVRAGSFLSNVGDFDAAFFGIGPREAQAM
                     DPQQRLLLEITWEALERASIDPTSLHGSDTAVFIGVTAQEYGPRIYEEAGGFSGHLAT
                     GTSVCVASGRVAYTLGLQGPALSVDTGCSSSLVAVHLAVQSLLSGECHLAVAGGATVV
                     CSPSSVVGLGRQGALAQDGRSKPFAASADGFGLAEGAAVLVLARLSHARALGYPVLAL
                     VRGTAVGQDGASNVLSAPSGPAQQRVIRQALRAADLTAGDIDVVEGHGTGTRVGDPIE
                     AKALQETYGEAHSSARPLLVGSVKSNIGHTQWAAGVAGMIKVIQSIRHGVVPATLNLD
                     SPTPQVDWSSGTIKLVGTACPWPDQAGRPRRAGVSSFGISGTNAHVIIEEAPAKRPTV
                     ESAPHPPAAMPWVLSAKSVQAVADQAARLRQFVEQRSDLDLHDLAYSLAATRTLFDYR
                     AVAVGTDRDELLSGLAAIATGTPAPNVVTGKAAATGGTVFVFPGQGSQWAGMAVELLD
                     SAPVFADQMQLCVAAFAEFVDWSLLEALRGEIGSPSLDRVDVVQPVLFAVMVSLAAQW
                     RALGIQPDAVIGHSQGEIAAAYVAGALSLREA"
BASE COUNT          460 a          869 c          941 g          506 t
ORIGIN      
        1 tacgttcggt gtcagaagaa ttcgagcgca tgggtgatct cattgccgcc gcggacgccg
       61 ccgcccacgc cgccatgaca tatcgtcgcc aaggcaaacg agggtccgca ctggcatgct
      121 cgacgagtgc agacgcccta gctgtcaggt gcggcggcgc ctgcacgccg gcgcttcgtc
      181 gcgccagcga ggccttgccg ctgaccgacc gcgaagcaga gatcgtgatg ctcatgagtg
      241 agggattgtc gtatcgagcg gttgccgagc gactgaccct ttccgtccgt actgtcgaga
      301 gtcacatgta ccgcgccatg ttgaagaccg gaacggccac ccgcgacgaa ctcgtcgccc
      361 tgctaccccg ccacgacgca ctgcagtagt acggcccgag gccgcggaga gtgcagtgct
      421 ccactactgc atccgtcata gcgcatcgga gcgacgattc cctcgcggag cgcgacaccc
      481 ggatgcggtg gccgtcgttt cgctaagacg gagtggaggg cacggcgatg agtaatggaa
      541 gcacatctgc ggggccgcgc catcgcacga cggggtccgt cacgcatccg tctgttagcc
      601 cgtatctcac cgctggtgtc gccgacgcca gcgcggcggt ccgaacgtcg gtggagacgt
      661 cggctgacaa cgcgtccacc gtcgagcagc atccccccgg ccaagtcgac aagaaaccga
      721 aacgcgccgc ggccaaagga gagcaagagc ccggacacga tcaatcggca acgcgaagcg
      781 cgtcgcagtg atgttcgatg cggcaccgat ggccgttgag ctcagcgagc acaagcgcgc
      841 gcatcagccc aacgagccga tcgccattct gggcatcgga tgtcgcttac cgggcggcgt
      901 cagctcaccg gatgacctgt ggcagctgct atccgaggaa cgtgacgccg tctcggagtt
      961 tcccaccgac cgcgggtgga atctcgatgt gctattcggg cctgaccccg atgcgcccgg
     1021 cgctacatac gtgcgcgcgg ggtcgtttct gtcgaatgtc ggtgatttcg acgcggcgtt
     1081 cttcgggatt ggtccgcgtg aggcccaagc catggacccg cagcagcggt tgcttctcga
     1141 aataacgtgg gaagcattgg agcgggcgag catcgatccg acttcgcttc atggcagtga
     1201 caccgccgtg ttcatcggtg tgactgcaca ggagtacggc ccccgcatct acgaggaggc
     1261 cgggggattt tccgggcatc ttgcaacggg tacttcggtc tgcgtcgcat cgggacgagt
     1321 tgcctacacg cttgggctac agggcccagc gctgtcggtc gacaccgggt gctcgtcatc
     1381 tttggtcgca gtacatcttg ccgtgcagtc gttgctctcg ggtgaatgtc acttggcggt
     1441 tgcgggcggt gcaacggtgg tgtgctcgcc cagcagtgtt gtcggactcg gccgacaggg
     1501 cgcgctcgcg caggacggcc gatccaagcc gttcgctgca tcggcggacg ggttcggcct
     1561 cgccgaaggc gccgcggtgc tggtgctggc ccgactgtcg catgcgcgcg cactcggata
     1621 tccagtactt gcgctcgtcc ggggcaccgc ggtcggacag gatggggcct ccaatgtgct
     1681 gtcggccccc agcggtccag cccagcagcg ggtaattcgt caagccctga gggcagcgga
     1741 cctgactgca ggtgacatcg acgtggtcga ggggcacggg actggcactc gagtcggcga
     1801 tccgatcgaa gcaaaagcgt tgcaggaaac ctacggcgag gcccactcct cggcccggcc
     1861 actgctggtc ggctcggtca agtcgaacat cggtcataca cagtgggcgg ccggcgtcgc
     1921 cgggatgatc aaggtcatac agtcgattcg gcacggcgtg gtgcctgcga ccttgaatct
     1981 ggattcacct acgccgcagg tggattggtc gtccggaact atcaagttgg tcggtaccgc
     2041 atgcccgtgg cccgaccaag ccggtcgacc gcggcgtgcc ggggtgtcgt cgttcggcat
     2101 cagcgggacg aatgctcacg tcattatcga agaggcgccg gcgaagcgtc cgactgtgga
     2161 gtcggcccct catccccccg cagcgatgcc gtgggtgttg tcagccaagt cggtgcaggc
     2221 ggtggctgat caagcggccc ggttgcggca attcgtcgag caacgcagcg acctcgatct
     2281 gcatgacctg gcgtactcac tcgccgccac tcggacacta ttcgactacc gcgcggttgc
     2341 ggtcggaacc gaccgtgacg agttgttgag cggattggcg gcgattgcca ccggcacacc
     2401 ggcaccgaat gtagttactg gtaaggccgc tgccaccggg gggacggtct tcgtctttcc
     2461 cggccagggg tcgcagtggg cgggcatggc ggtggaactc ctggactctg ctccggtgtt
     2521 cgccgatcaa atgcagctct gcgtggccgc tttcgccgag tttgtcgatt ggtcgctgct
     2581 cgaggctttg cgtggcgaga tcggctctcc gagcctcgac cgggtggatg tggtgcagcc
     2641 cgtgcttttc gcggtgatgg tgtcgctggc ggcgcaatgg cgagcgctag gaatccagcc
     2701 cgatgccgtc atcggccatt cgcagggcga gatcgccgcc gcctacgtcg cgggcgccct
     2761 gtctttgcgg gaggcc
//
LOCUS       DASRJA010000682         8485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3193594,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000682 DASRJA010000000
VERSION     DASRJA010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3193594"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VFC01_30615"
     CDS             <1..1086
                     /locus_tag="VFC01_30615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZN84004.1"
                     /translation="RAMAVMASGVGDRLWPNDVRLAAPPNPVVAVPEELPELDREWRA
                     AFDAAGARLTDAGARIVTVDIAPFLAAAKLLYDGALVSERYAAVGEFIGAHPDAALDP
                     TVRRIISAARDIPAHRLVRDRREVERLHAVAMAALDGVDALVVPTAPMHPRIEEVAAD
                     PVGVNSRMGTYTNFCNLFDLCGVAVPAGTAGEAQFGITVLARAFEDAVALDIAALVSN
                     DQAPADVWPLAAADSVELVVFGAHLKGGPLVHQLTDLGARWTGEVTTAPRYRMSVLPT
                     TPAKPAITRVPDGAMGAALLGHRWLLSPAALGRFLAALPAPMQLGKVEFDDGTWRTAF
                     GCDGAAATGVDISAYGSWPAAIAAGAV"
     gene            complement(1096..1284)
                     /locus_tag="VFC01_30620"
     CDS             complement(1096..1284)
                     /locus_tag="VFC01_30620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84005.1"
                     /translation="MSSNYVDDRGRRSIPFRYVWPSELDLMARIAGMVLVDRWEDWDR
                     KPFTHESTRHVSVWRKPD"
     gene            complement(1281..1688)
                     /locus_tag="VFC01_30625"
     CDS             complement(1281..1688)
                     /locus_tag="VFC01_30625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN84006.1"
                     /translation="MYEPDGYFGEDVAATYDEATADMFDESIAGPAVELLAELAGEDR
                     VLEFGIGTGRIALPLAQRGVEVHGIDLSRAMVSRLHAKPGGAAIGVTIGDFADARVGV
                     PALRPLPPAKGWFRSRSVTSTGPMTYTTAPHRR"
     gene            complement(1788..2246)
                     /locus_tag="VFC01_30630"
     CDS             complement(1788..2246)
                     /locus_tag="VFC01_30630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84007.1"
                     /translation="MRNTLVTRLPMMGLAGAALLAFGLSAASPALADTDAGTATVRVP
                     AVMWHGDPPPPGMVWDVTLNGDKGGELAKQHTSGGRPDPAFTFNNVPAGQIYTASIRY
                     NNTNENLHCHGQTSAMERQPAQPSSAGQTIDLSPVVMIFDDASGRCHASP"
     gene            complement(2412..4415)
                     /locus_tag="VFC01_30635"
     CDS             complement(2412..4415)
                     /locus_tag="VFC01_30635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014878112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZN84008.1"
                     /translation="MTTVAPSVRSSAVWPVLVGIGVLAGAVAAGIGALSLADALTATG
                     LPNPGPATTYGLPFVRAAGEIAAVLAAGSFLFAAFLVPPQTNGVLDAAGYRALRLGTA
                     ASAVWTVCAALLVPMTVSDVSGHPLRENLNPVDIWSAASLVDTASAWRWTAFIAAAVT
                     LASLPVLRWGWTPVLLAGALITLVPLGLTGHSSAGGAHDLATNSLLIHLTAGALWAGG
                     LLALLAHAMRSGEHADLAARRFSALALWCFVAMAGSGVVNALVRIQLPELVRSEYGWL
                     VIGKLTALCVLGLIGWRQRRSGLVALKTDQEARAPLIRLALVEAAVFGATFGIAVGLG
                     RTPPPPMLTEPSPVEVAIGYGFDGPPTLARVLFDWRFDLIFGTAAIVMALLYLVGVRR
                     LRRRGDAWPTGRIVAWLCGCAVLLFTTSSGLGRYMPAMFSMHMVAHMLLSMLVPILLV
                     LGAPMTLALRALPTAGRGEPPGPREWLLAALHSRVSRFLTHPIVATVVFVAGFYGLYL
                     GGVFDAAVSNHAAHMLMNIHFLLSGYLFYWVVIGIDPTPRQIPQLGKVAMVFASLPLH
                     AFFGVVLMGMQTVLGETFYRSLGLSWHTDLLGDQRLGGGIAWAAGEVPLVVVMIALLI
                     QWRRSDQRVAKRLDRAADRDDDAELAAYNAMLAELARRDTPQR"
     gene            complement(4438..6786)
                     /locus_tag="VFC01_30640"
     CDS             complement(4438..6786)
                     /locus_tag="VFC01_30640"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase"
                     /protein_id="HZN84009.1"
                     /translation="MKISADQFAPFTATDDALVLASVSSPAEEELLNDWLERQRREHP
                     DSNVDVLRLPADDDPPPGTLARLVEELDADADRSVVPVRVFWVPGGLPTRSKIVSFLS
                     GRDTYRPPEILQKRILRKDPSRARVVAGEPAKVSELRQQWNETTVAENPREFARFVIR
                     RAILAIERVELRLLGPEYKSPRLIKPEILASSRFRDGLERIPGATVEKAGEMLDELST
                     GWSRFSVDLIPSLGRAIFSRGFDPNVDYDRAEVETMRRGLERHPAVLLFSHRSYLDGV
                     IVPVAMQENRLPPVHTFAGINLSFGFMGPLMRHSGVIFLRRKLDDPLYKYVLRQYVGY
                     IVEKRFNLSWSIEGTRSRTGKMLPPKLGLLAYVADAYLDGRSDDILLQPVSISFDQLH
                     ETAEYARYARGGEKTPEGLSWMYNFIKAQGERNYGKIYVRFPEAVSMREYLGAPHGPM
                     TTDEAAKRLALQKMAFEVSWRILRATPVNATGLVSALLLTTRGVALTLDQIHHTLQDS
                     LDYLERKQAPMTNSALRLRTADGVRAALDALSNGHPVTAVEGGRETVWRIAPEDEHEA
                     AFYRNTVIDAFMETSIVELALAYAWRVESDRLEAFWSQAMRLRDLLKFDFYFADSTAF
                     REHVAEEMSWHEDWEAHVAAGGDQIATLLRAKKPLIASAMLRPFFEAYEIVSDVLRDA
                     PAEIGEKELTKKALGVGRQYVAQNRVRSNEAVSALLFATARQVVADQHLLKPADDLVE
                     RRTAFRDELRAVLSDMDRVEQIAREQFYAREMEFRRLRTETT"
     gene            complement(6783..8441)
                     /locus_tag="VFC01_30645"
     CDS             complement(6783..8441)
                     /locus_tag="VFC01_30645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase/lysophospholipid
                     acyltransferase family protein"
                     /protein_id="HZN84010.1"
                     /translation="MTSANGQPNPRTMRLPGSVAEIEGSPPGPEVGAFFDLDGTLVAG
                     FTGVIMTQDRLRRRQMSVGEFIGMVQAGLNHQLGRSEFEDLIGKGARMLRGNSLEDID
                     ELAERLFVQKIVGRIYPEMRELVRAHMARGHTVVLSSSALTVQVEPVARFLGIANVLS
                     NKFELDDNGCLTGEVKTPIIWGPGKARAVQAFAAKNGVDLSKSYFYADGDEDVALMYL
                     VGNPRPTNPAGKLAAVAAKRGWPVLRFTSRSGSNPVSQLRTAAGIASMVPIAAGALGL
                     GLLTRNKRTGVNFFTSTFGRTLLTVTGVNLNVLGEENLTVQRPAVFIFNHRNQADPMI
                     AGALVKDNFTSVGKKELENDPIVGTMGKIMDAAFIDRDDPKKAIEGLKKVEELARKGL
                     SILIAPEGTRLDTTEVGPFKKGPFRIAMSAGIPIVPIVIRNAEVIAARDSSTFNPGTV
                     DVVVYPPIPVEDWTHDNLTDRIDEVRQLYLDTLNDWPHDELPTPALYTRKKAPAKKSA
                     TKASAKKTPAKAAKATVKKAAEKKPVKKAAAKKTTKKAAAKGRP"
BASE COUNT         1469 a         2833 c         2728 g         1455 t
ORIGIN      
        1 cgcgcgatgg cggtgatggc ttccggcgtc ggggatcgac tgtggccgaa cgatgttcgg
       61 cttgcggcgc ccccgaatcc cgtcgttgcg gttcccgagg agctgcccga gttggaccgg
      121 gagtggcgcg ccgcgttcga cgccgccggc gcccggttga ccgacgcggg tgcacgcatc
      181 gtcaccgtgg acatcgcacc gttcctggcg gcggccaagc tgctgtacga cggcgcattg
      241 gtgagcgaac gctacgccgc cgtaggcgaa ttcatcggcg cgcatccgga cgccgcgctg
      301 gatccgacgg tgcggcgaat catcagtgcg gcgcgcgata tcccggcgca tcgactggtg
      361 cgcgaccgcc gtgaagttga gcggttgcat gccgttgcga tggccgcgct cgacggcgtc
      421 gacgcgctcg tggtgccgac cgctccgatg catccccgca tcgaagaggt ggccgccgat
      481 cccgtcggcg tcaactcgcg catgggcacc tacaccaact tctgcaatct gttcgacctg
      541 tgcggggtgg ccgtccctgc gggtacggcg ggggaagctc agttcggaat cacggtgcta
      601 gcgcgtgcgt tcgaggatgc ggtggcattg gacatcgcag ctctggtgtc gaacgaccaa
      661 gcgcccgccg atgtttggcc gctggcggcg gccgactcgg tcgagctggt ggtcttcggg
      721 gcgcacctga agggcggacc gctggttcac cagctcaccg acctcggtgc gcggtggacc
      781 ggcgaggtca cgacggcgcc gcgctaccgg atgtcggtgt tgccgaccac tcccgccaag
      841 ccggcgatca cgcgggtacc tgacggcgct atgggcgcgg cgctactcgg gcatcggtgg
      901 ctgttgtcgc ccgccgcgct gggccggttc ctagcggcgc tgccggcgcc aatgcaactc
      961 ggcaaggtcg aattcgacga cggcacatgg cgaactgcgt tcggctgcga cggcgccgca
     1021 gccacggggg tggatatcag cgcctacggc agctggccgg cggccatcgc ggcaggcgcc
     1081 gtttgacgcc gctcgtcagt cgggcttccg ccacaccgat acgtgacgtg tgctctcatg
     1141 cgtaaagggc ttgcggtccc aatcttccca gcgatcgacc agaaccatgc ccgcgatgcg
     1201 tgccattagg tccagctcgg acggccacac ataccggaac gggattgagc gccggccgcg
     1261 gtcgtcgacg tagttggaac tcatctcctg tgtggcgcag tcgtatacgt cataggccca
     1321 gtcgacgtca ccgaccgcga acggaaccac cccttggccg ggggcagcgg acgcaatgcg
     1381 ggcacgccga cgcgtgcatc ggcgaagtca ccgatcgtca caccgatggc ggctccgccc
     1441 ggctttgcat gtagccgact gaccattgcc cgggacaggt cgatgccgtg cacctcgacg
     1501 ccgcgctgtg cgagtggcag cgcgatacgc cctgtcccaa taccgaattc aagcacccga
     1561 tcctcgccgg ccagttccgc caagagttca accgccggac cggcgatgct ctcgtcgaac
     1621 atgtctgccg tcgcttcgtc gtaagtcgcg gcgacgtctt cgccgaaata gccgtctggt
     1681 tcgtacataa acgaacgacg gtacgcggca agaggccagt tatcacatgc atttatcgcg
     1741 cacggcgtgc aataactggc cacccgacgg gctagggcga gctgtcgcta ggggctggcg
     1801 tgacacctgc cactggcatc gtcgaagatc atcacgaccg gcgagaggtc gatggtctgc
     1861 ccagcgctcg agggctgcgc cggctggcgt tccattgcgg acgtctgtcc atggcaatgc
     1921 agattctcat tggtgttgtt atacctaatg ctggcggtat agatctgccc ggcaggcacg
     1981 ttgttaaagg tgaacgctgg atccgggcgt ccaccggagg tgtgctgctt ggcaagctca
     2041 ccgcccttgt ctccgttcaa cgtgacatcc cagaccatgc ctggcggcgg cggatcgcca
     2101 tgccacatca cggcagggac gcgcacagtg gccgtcccag cgtcggtgtc agccagcgct
     2161 ggtgacgccg cgctgagtcc gaaggccaat agcgcggcgc cggccaaccc catcatcggg
     2221 agccgcgtca caagtgtgtt cctcattggt gtgtcctttc gcatggacaa gtacgggcga
     2281 acatgtcacg cgcccaaact atgagtcgga cgaatcgatg gctcgcttgt gtgcacaacc
     2341 cgcaatgacg gcgattccca gtctttttcg cattgtgccc agtcggtcct gggcgcatca
     2401 acgcacgtcc gctaacgctg cggtgtatcc cgccgtgcca gctcggcgag catcgcgttg
     2461 tatgcggcca actccgcgtc gtcgtcgcgg tcggcggcgc ggtcaaggcg cttggccacc
     2521 cgctggtcgc tgcgtcgcca ctggatcagc aaggcgatca tcaccacgac aagtggcact
     2581 tcgccagccg cccaagcgat tccgccaccc agtcgttgat cgccgagcag gtcggtgtgc
     2641 cagcttagcc ccagtgaccg atagaacgtc tcgcccagaa ctgtctgcat gcccatcaac
     2701 acgacaccga agaacgcgtg caacggcagc gacgcgaaca ccatcgcgac cttgcccaac
     2761 tgcggaatct gccgaggtgt cgggtcgatg ccgatcacca cccagtagaa gaggtatccg
     2821 ctgagcagga aatggatgtt catcagcatg tgcgcggcat ggttgctcac cgctgcgtcg
     2881 aacacgccgc ccaggtaaag cccgtagaag cccgcgacga acaccacggt ggccacgatc
     2941 gggtgggtga ggaaccgcga cacacgcgaa tgcaacgccg ccagcagcca ttcccgcggc
     3001 ccaggcggct ctccccgtcc cgcggtcggc agcgcgcgca gcgcaagcgt catcggcgcg
     3061 cccaacacca gcaggatcgg caccagcatg gacagcagca tgtgtgccac catgtgcatc
     3121 gaaaacatcg ccggcatgta gcggcccagc cccgacgacg tggtgaacaa cagcaccgcg
     3181 cagccgcata gccacgcgac aatgcgcccc gtcggccacg cgtcgccacg gcggcgcaac
     3241 cggcgcaccc cgaccaggta cagcagcgcc atgacgatcg ccgcggtgcc gaagatgagg
     3301 tcgaaccgcc agtcgaacaa cacccgcgca agcgtcggcg gcccgtcaaa gccgtaaccg
     3361 atcgccacct cgacaggact cggctccgtc agcattggcg gcggcggtgt gcggcccaac
     3421 ccgaccgcga taccgaacgt cgctccgaag acagccgcct cgaccagtgc cagccggatc
     3481 agcggcgcgc gggcttcctg atcggtcttc agcgccacca gcccgctgcg acgctgacgc
     3541 catccgatca ggccgagcac acacagtgca gtgagcttgc cgatcaccaa ccacccgtat
     3601 tcggagcgca caagctcagg cagttggatc cgcaccagcg cgttgaccac accgctgccc
     3661 gccatcgcca cgaagcacca cagcgccagc gccgaaaacc gccgggccgc gaggtccgcg
     3721 tgttcccccg aacgcatcgc gtgagccagc aacgccagca ggccgcccgc ccataacgcg
     3781 cccgcggtga gatgaatgag caggctgttc gtggccagat cgtgtgcgcc gccggccgac
     3841 gaatgtccgg tgaggcccag cggtacgagc gtgatcaatg cccccgccag cagcaccggc
     3901 gtccaccccc accgaagcac cggaaggctg gcgagcgtga ccgcggccgc gatgaatgcg
     3961 gtccagcgcc acgccgaggc ggtgtcgacg aggctggccg ccgaccagat gtcgaccgga
     4021 ttgaggtttt cgcgcagcgg gtgccccgac acatccgaca ccgtcatcgg cacgagcagc
     4081 gccgcacaga ccgtccacac cgccgacgcg gccgtaccga gccgcagcgc gcgatacccg
     4141 gccgcgtcga gcacgccgtt ggtctgcggc ggcaccagaa acgccgcgaa caggaacgat
     4201 cccgcggcca gcacggctgc gatctcgccc gccgcccgca cgaacggcag cccgtaggtg
     4261 gtcgcaggcc cggggttggg cagcccggtg gcagtgagcg cgtcggccag cgacagcgcg
     4321 ccgatcccgg cggccacggc cccggccagc actccgatgc cgaccagcac gggccacacc
     4381 gcgctgctgc gcacgctcgg ggccaccgtg gtcatgcgcc cagcgtatgg cgtgtagcta
     4441 ggtggtctcg gtgcggaggc ggcggaactc catctcacgc gcatagaact gttcgcgagc
     4501 gatttgctcg accctatcca tgtcacttag gacggcgcgg agctcgtcgc ggaacgcggt
     4561 gcgccgctcg acaaggtcat ccgcgggttt cagcagatgc tgatcggcga ccacttgccg
     4621 cgcagtcgcg aacagcagcg cagagacggc ctcgttgctg cgcacccggt tctgcgcgac
     4681 gtactgtcgg ccaacgccaa gggctttctt ggtcagttcc ttctcgccga tttccgcggg
     4741 cgcgtcgcgc agcacatccg acacgatctc gtacgcctcg aagaacggcc gcagcatcgc
     4801 gctggcgatt agcggcttct tggcgcgcag cagcgtggcg atctggtcac cgcccgcggc
     4861 gacgtgggcc tcccagtcct catgccacga catctcctcg gccacatgct cgcgaaacgc
     4921 ggtggagtcg gcgaagtaga agtcgaactt caggagatcc cgcagccgca ttgcctgcga
     4981 ccagaacgct tcgagccggt cggattccac ccgccacgca tacgcgagag ccaactcgac
     5041 gatcgacgtt tccatgaacg catcgatgac ggtgtttcgg tagaaggccg cctcgtgctc
     5101 gtcttcgggc gcgatcctcc agacggtctc cctgccgccc tcgacggccg taacggggtg
     5161 gccgttggac aacgcatcca gcgccgcgcg gacgccgtca gctgtgcgca accgcaacgc
     5221 gctgttggtc attggggctt gcttgcgttc cagataatcc aacgagtcct gcagggtgtg
     5281 atggatctgg tcaagcgtca gcgcgacacc gcgggtcgtc agcaacaggg ccgacacaag
     5341 ccccgtcgcg ttcaccggtg tcgcccgcag gattcgccat gacacttcga acgccatctt
     5401 ctgcaacgca aggcgtttgg cggcttcgtc ggtggtcatc ggcccgtgtg gcgcacctag
     5461 gtactcgcgc atcgacaccg cttcgggaaa gcggacgtag atcttgccgt agttgcgctc
     5521 gccctgcgcc ttgatgaagt tgtacatcca cgacagaccc tcgggcgttt tctcgccgcc
     5581 ccgcgcgtac cgcgcatact cggccgtctc gtgcaattgg tcgaagctga tcgacaccgg
     5641 ttgcaagagg atgtcgtcgc tgcgcccgtc gaggtaggcg tcggcgacgt aggcgagcag
     5701 accgagcttg ggcggcaaca tctttccggt gcgcgaccgg gtgccctcga tcgaccagct
     5761 gaggttgaac cgtttctcga cgatgtagcc gacgtactgt cgcagcacgt acttatacag
     5821 cggatcgtcg agcttgcgtc gcaagaagat gacgccggag tggcgcatca gcgggcccat
     5881 gaagccgaac gacaagttga tgcccgcgaa cgtgtgaacc ggaggtaatc ggttctcctg
     5941 catcgccacc ggcacgatga cgccgtcgag ataggaccgg tgcgagaaca acagtacggc
     6001 gggatgacgt tccaatccgc gtcgcatcgt ttcgacttct gcgcggtcgt agtcgacgtt
     6061 gggatcgaag ccgcggctga agatggctcg tcccaaggac gggatcaggt cgacagaaaa
     6121 ccggctccag ccagtcgaga gctcgtcgag catctcgccg gccttctcca ccgtcgcgcc
     6181 gggaatgcgt tccagaccgt cgcgaaaccg agacgacgcc aggatctcgg gtttgatcag
     6241 tcgcggcgac ttatactcag ggcccagcag gcgcaattcg acacgctcga tcgccaggat
     6301 cgcgcggcga atgacgaatc gcgcgaattc cctggggttt tcggcgacgg tggtctcgtt
     6361 ccactgctgg cgaagttctg acaccttcgc cggctcacca gccaccactc gggcccgcga
     6421 cgggtccttg cgcaaaatcc gcttctgcag gatctccgga gggcgatacg tatcccggcc
     6481 cgacagaaac gagacgatct tggaccgcgt gggcagaccg ccggggaccc agaacacgcg
     6541 caccgggacg accgaccgat ctgcgtctgc gtccaactcc tcgacgaggc gcgccagggt
     6601 tcccggcggt gggtcgtcgt cggcaggcaa ccgcagcacg tcaacgttgg agtcggggtg
     6661 ctcgcgccgc tggcgttcca gccagtcgtt aagcagttcc tcttcggccg gcgacgacac
     6721 cgacgccagc accagcgcgt cgtcagtggc tgtgaacggc gcgaactgat cggcggagat
     6781 tttcacggcc gacctttcgc ggcagccttt ttcgtcgttt tcttagcggc cgcctttttc
     6841 acgggcttct tctctgcggc tttcttgacg gtcgccttgg ccgccttggc cggcgtcttc
     6901 ttcgctgacg ccttcgtcgc ggatttcttc gcaggcgcct tcttacgggt gtacagcgcc
     6961 ggtgtcggca gctcgtcgtg cggccagtca ttgagcgtat ccaggtacag ctgacggacc
     7021 tcgtcgatgc ggtcggtgag gttgtcgtgg gtccagtcct cgacggggat cggcgggtac
     7081 accactacgt cgacggtgcc ggggttgaat gtgctcgagt cgcgggccgc gatgacctcg
     7141 gcattgcgaa tcacgatggg cacgatcggg attccggcgg acatcgcgat gcggaacggt
     7201 cccttcttga acgggcccac ctccgttgtg tccagccgcg tgccctccgg tgcgatcagg
     7261 atcgacagcc ccttgcgtgc cagctcctca accttcttca gtccttcgat cgccttcttg
     7321 gggtcgtcgc ggtcgatgaa ggcggcgtcc atgatcttgc ccatcgtgcc aacgatggga
     7381 tcgttctcga gctccttctt tccgaccgac gtgaagttgt ccttgaccaa tgctcccgcg
     7441 atcatcggat cggcctggtt gcggtggttg aagatgaaga cggcaggccg ctgcacggtc
     7501 aggttttcct cgcccaacac gttgaggtta accccggtga cggtcaacaa ggtgcggcca
     7561 aaggtcgaag tgaagaagtt gacgcccgtg cgtttgttac gcgtcagcag gcccagcccc
     7621 agcgcgcccg cggcgatcgg caccatggac gcgataccgg cggcggtgcg cagttgcgag
     7681 accggattgc ttccactgcg gctggtgaat cgcagcacgg gccaaccgcg tttggcggcc
     7741 accgctgcga gcttgcccgc gggattagtt ggccgcgggt tgccgacgag gtacatcagc
     7801 gcgacgtctt cgtcgccgtc ggcgtagaag tagctcttcg acagatcgac gccgttcttt
     7861 gcggcgaatg cctgtaccgc cctggccttt ccggggcccc agatgattgg cgtcttcacc
     7921 tcgccggtca ggcagccgtt gtcgtcgagc tcgaacttgt tgctcaacac gtttgcgata
     7981 cccaggaacc gggcaacagg ctcaacttgc accgtcaacg ccgacgagct gagcacgacg
     8041 gtgtggccgc gggccatgtg ggcacggaca agttcgcgca tctcgggata gatgcggccg
     8101 acgatcttct gcacgaagag acgttcagcc agttcgtcga tgtcctccaa tgagttgccg
     8161 cgcagcatgc gcgcaccctt gccgatgaga tcctcgaact cggaacgacc gagctgatga
     8221 ttgaggccgg cctgcaccat gccgatgaat tcgcccactg acatctggcg acggcgcagc
     8281 cgatcttggg tcatgatcac accggtgaac ccggcgacga gggtgccgtc gaggtcgaag
     8341 aacgcgccga cctcggggcc aggtggactg ccttcgattt cggcgaccga ccctggcagc
     8401 cgcatggtcc gtggattggg ctgaccgttg gccgaagtca ttgcgccgca cttccgttcg
     8461 gtgacacagg ggtgggttct agggc
//
LOCUS       DASRJA010000683         6725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2349263,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000683 DASRJA010000000
VERSION     DASRJA010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6725
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2349263"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VFC01_30650"
     CDS             complement(<1..791)
                     /locus_tag="VFC01_30650"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZN84011.1"
                     /translation="MPDSTRREFLKVQLGFGFAGLAFPLRSSDIWPAAPASRHLKGAR
                     AQAVIQLHLGGGLSHLDTFDPKPEAPVEVRGPFGTVKSKLQGEPLSDLLRRTAEIADK
                     IAVIRSVTHTEADHDRGTHTMLTGFQPSPAITYPSLGAVVAHELGGRNDLPPYVCVPS
                     AGSFMGTGYLSAAHAPFSVGDNPASRRFQVRDLNAPKDVDEARRARRKELLHELDAGF
                     SELGAGDVLVANEAFYRQAYAMIESPAARAAFDLASEPDAMKDRYG"
     gene            1215..3245
                     /locus_tag="VFC01_30655"
     CDS             1215..3245
                     /locus_tag="VFC01_30655"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZN84012.1"
                     /translation="MQRLATTLAVMAIAATATAQFTVTVPNGTASVEGNTSNAFPWGR
                     GGTGLRHVSIYDSTNFTLQGITTPIIISQLRYRPNASASSLASSYPSACTVQLSTCPI
                     DQSGVVAGLPLSTYVGANVTTCFSGPMSWPAAAAVPGPCPFLITVPFSTNFVYDPTAG
                     DLTIDTDIPIQAFTGTSLQLDVQSTGSLSSRAYISTGYPTGATAITQNHGVVCEITYV
                     PAAGLYPAFTGTPRTGPVGQLVNFTDQTYTSDPGGVTSYAWDVNGDSVTDYTTQNCSH
                     TYTAEGNYNVTLTVTSAMFGTQSLTKTNYIVIDAVDASFTSAVLTATLVAFTDTSTGN
                     PTSWAWDFTNDGIVDSNLQNPAFLYPAPGQYTCKLTVTDAFSNDTVTQNLGIGIIPVP
                     GFGSTFSSATATRGLWFQSPTRFSIITASVPDETANGTQNVAIYRLAAAPPVFSATAT
                     GGLEFYANLQPSGTPIPCVVSYDVGEFVGVLGACGSTTMLNSYATPAGPFASSVLGNP
                     TTLTRFGTQFNINTAGPNNPYWQEPAGAISRVVLGVTACAAIPYGVGSPSGLGPAAPK
                     MRGTALPFIGQTAVHTVTNNDALVLQLMVGGFGRLSIPVPPFGTILIGSVDLIDVMNG
                     GVPAGPGNTTWSFAIPALPALVGQSINWQNANIVIPTGEWSMSNGVEWWIGN"
     gene            3499..5526
                     /locus_tag="VFC01_30660"
     CDS             3499..5526
                     /locus_tag="VFC01_30660"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZN84013.1"
                     /translation="MQRFSFLLAALTVAATASAQFTITVPNGTATVEGNTSNAFPWGR
                     GGTGLRHVSIYDSSNFTLQGVTTPIIISQLRYRPNTNTSSLASNYPIGGTVQLSTCPI
                     DQSGVVAGLPLSTYVGANVTTCFSGPMSWPAAAATAGPCPWLITVPFSTNFVYDPNQG
                     DLTIDTDIPIQAFTGTSLQLDVQSTGSLSSRAYISTGYPTGATAITQNHGVVCEITYV
                     PATGLHPQFSATPRTGPVGQLVQFTDQTYTSDPGGVTSWAWDVDGDSVTDYTTQNPSH
                     TYTTEGNKTVTLTVTSAMFGSASITKTNYIVIDAVDASFTSSVLTATLVAFTDTSTGN
                     PTSWAWDFENDGIVDSNLQNPAFFYPAPGQYTCRLTVADAFSNDTTTLTFGIGIIPVP
                     AFGSTFSGQLTRGFWFQSPTRFSVVSASVPDETANGTQNVAIFRLAAAPPVFSATATG
                     GLEFVALSQPSASPIPCVVSFDVGEYVGVLGACGTATMINSYATPAGPFASSVLGNPT
                     TLTRFGTQFNINTSGANQAYWQEPGGQISRVVLGVTACAAIPYGTGSPSGLGPAAPSM
                     RASALPFVGQTAVHQVTQSDALVLQLMVGGFGRLSVPVPPFGTILIGSLDLIDVMNGG
                     APVGPGTYPWSFAIPPNPVLVGQSINWQNAHLVIPTGEWAMSNAIEWWINN"
     gene            5690..6385
                     /locus_tag="VFC01_30665"
     CDS             5690..6385
                     /locus_tag="VFC01_30665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84014.1"
                     /translation="MDETRLSRQFLRVFLVFLILTALLAISIVLFEDRNQTRWRILGT
                     SSVISVASVAAMACAAFRERGQLRWLGNVGMSLCFAAAAGIVVAIWDFVHDETYGRFL
                     GCLSTVAGFTALGQLLWLMPLIQPHRWVQKATTYTIAVLTMLICLQIAARITDAAWDR
                     LWVAIAIVVTLQVLAHPILWQLGRRAGGAAQRRLLLSHERDDIWTDGAGRRYSVRPLL
                     GDMAAGDLAGDQG"
     gene            complement(6386..>6725)
                     /locus_tag="VFC01_30670"
     CDS             complement(6386..>6725)
                     /locus_tag="VFC01_30670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84015.1"
                     /translation="VRLVVREGGLLVRGGGVDTVVTLELMAQAVAACLGYEAFLHGGG
                     VRVGMVVACRKFAIARSRIEVGERLDIHVTRLRGTDDASTFEAEVHDGRGALVSSAVM
                     TLVHGDKPPD"
BASE COUNT         1105 a         2380 c         2166 g         1074 t
ORIGIN      
        1 ccgtagcggt ccttcatcgc gtcgggttcg ctggcgaggt cgaacgccgc gcgcgcggca
       61 ggcgactcga tcatcgcgta ggcttggcga tagaacgctt cgttcgcgac gagcacgtcg
      121 ccggcgccga gctcggagaa gcccgcgtcg agctcgtgca gcagctcctt gcgtctcgcg
      181 cgccgcgcct cgtcgacgtc cttcggcgcg ttgaggtcgc ggacctggaa gcgccgggac
      241 gcgggattgt cgccgaccga gaacggagcg tgcgcggcgc tcaggtagcc ggtgcccatg
      301 aagctgcccg cggacggaac gcagacgtag ggcggcaggt cgttgcgccc gccgagctcg
      361 tgggcgacca cggcgccgag gctcgggtag gtgatcgcgg ggctcggttg gaacccggtc
      421 agcatcgtgt gcgtgccgcg gtcgtgatcc gcttcggtgt gcgtcaccga ccggatcacc
      481 gcgatcttgt ccgcgatctc cgccgtgcgg cgcagcaggt ccgacagcgg ctcgccttgc
      541 agcttggact taacggtgcc gaacgggccg cgcacctcga cgggcgcctc gggcttcggg
      601 tcgaaggtgt cgaggtggct cagcccgccg ccgagatgca actggatcac cgcctgggcg
      661 cgcgctccct tcaggtgacg cgacgcgggc gcggcgggcc agatgtcgct cgatcgcagc
      721 ggaaacgcga gacccgcgaa gccaaagccg agctggacct tcaggaactc gcggcgagtg
      781 gagtcgggca tgattggtca ggagtggcga ccgccgcatt ctaggcagcc ctcctcggac
      841 gagaggcggc ctcgttcgtt tggtgccagg tccaggaact ggaggacacg gcgcaaggag
      901 gcacaagcgg ctggggcatc tccgcaggca cgtgcaaccg acagtgccag gtccactcga
      961 atccaccagt gccgtctctt gccctggctt cgcgagcccg gtgcgcttgg cacgtcgatg
     1021 actgcggcga ttcgcgccaa ggggtgcgcg tgagcaggca tgttcggtcc tgcgcatctg
     1081 ctcgcgccgc gacgcgcgta tgacatttcg aggattgcat gggggtgcca ttagttccca
     1141 ttgcggctac ccttctgcgc acctgaccgc ccccatggcg gggacgcacc aaaaaccact
     1201 caaagggaaa atccatgcaa cgtctcgcaa cgacgcttgc cgtgatggca attgctgcca
     1261 cggcgacagc gcagttcacc gtcacagttc cgaacggcac cgccagtgtc gaaggcaata
     1321 ccagcaacgc gttcccctgg ggccgcggtg gcacgggcct gcgccacgtg agcatctacg
     1381 acagcacgaa cttcacgctg cagggcatca cgacgccgat catcatcagc caattgcgct
     1441 accgcccgaa cgccagcgcg tcgtcgctcg cgtcgagcta cccgagcgcg tgcacggtgc
     1501 agctgtcgac ctgcccgatc gaccagagcg gagtcgtcgc gggcctgccg ctcagcacct
     1561 acgtgggcgc caacgtcacg acgtgcttca gcggtccgat gtcgtggccc gcggccgccg
     1621 cggtgccagg cccgtgcccc ttcctgatca cggtcccgtt cagcacgaac ttcgtctacg
     1681 acccgacggc gggcgacctg acgatcgaca cggacatccc gatccaggcg ttcacgggca
     1741 cgagcctgca gctcgacgtg cagtccacgg gctcgctgag cagccgcgca tacatcagca
     1801 ccggttatcc gacgggcgcg acggcgatca cgcaaaacca cggcgtggtg tgcgagatca
     1861 cctacgtgcc cgcggcgggg ctctatcccg ccttcacggg gacgccgcgt accggcccgg
     1921 tcggccagct cgtcaacttc accgaccaga cctacaccag tgacccaggt ggcgtcacgt
     1981 cgtacgcctg ggacgtgaac ggcgactcgg tcaccgacta cacgacccag aactgctcgc
     2041 acacctacac ggccgagggc aactacaacg tcacgctgac cgtgacgagc gccatgttcg
     2101 gcacgcagtc gctgaccaag accaactaca tcgtgatcga cgccgtcgac gcgagcttca
     2161 cgtccgcggt cctgactgcc acgctcgtgg ccttcaccga cacgtcgacc ggcaatccga
     2221 ccagttgggc gtgggacttc acgaacgacg gcatcgtcga cagcaacttg cagaacccgg
     2281 cgttcctcta tccggcgccc ggccagtaca cgtgcaagct gacggtcacg gacgcgttca
     2341 gcaacgacac ggtgacgcag aacctcggca tcggcatcat cccggtgccc ggcttcggct
     2401 cgacgttcag cagcgcgacg gcgacgcgcg gcctctggtt ccagtcaccg acgcgcttct
     2461 cgatcatcac ggccagcgtg cccgacgaaa ctgccaatgg cacgcagaac gtcgcgatct
     2521 accggttggc cgcggcgccg ccggtcttct ccgccacggc taccggcggc ctggagttct
     2581 atgcgaacct ccagcccagc gggaccccca tcccgtgtgt ggtcagctac gacgtaggtg
     2641 agttcgtcgg cgtgctcggc gcctgcggct cgacgacgat gctgaactcc tacgccacgc
     2701 cagcgggtcc gttcgcgtcc tcggtgctcg gcaacccgac cacgctgacg cgctttggca
     2761 cgcagttcaa catcaacacg gccgggccca acaaccccta ctggcaggag cctgccggcg
     2821 ccatcagccg cgtggtcctc ggcgtcacgg cgtgcgccgc gatcccctac ggggtcggct
     2881 cgccctcggg cctgggcccg gcagccccga agatgagagg gactgcactg ccgttcatcg
     2941 gtcagaccgc cgtgcacacg gtcacgaaca acgacgcgct cgtgctgcag ctgatggtcg
     3001 gtggcttcgg tcgtctgtcg atcccggtcc cgccattcgg cacgatcctg atcggcagcg
     3061 tcgacctgat cgacgtcatg aacggcggcg tgccggccgg gccgggcaac actacgtggt
     3121 ccttcgcgat cccggcgctc ccggccctcg tgggccagag catcaactgg cagaacgcca
     3181 acatcgtgat cccgaccggc gagtggtcga tgtcgaacgg cgtcgagtgg tggatcggca
     3241 actagtcgcg acctgaccag cccggcgaag cgcccgccaa gggcgctggc agtgagaacg
     3301 aggccgctcc ctcggacgaa ggggcggcct cgcttcgttt ggcccgttcg gtcctgcgca
     3361 tcacctgggt gttgaacgtg cgcgagggga atctctggtc tttcatcccc ctgcctcaca
     3421 ggtaccgttc ctcgcttcgc gcgacccctc ggcggggtcg cttcaggaat ctgactgtcc
     3481 ccaaaccaag agaacaccat gcaacgtttc tcattcctgc ttgcagcgtt gactgttgct
     3541 gcgaccgcct cggcccagtt caccatcacc gttccgaacg gaactgccac tgtcgaaggc
     3601 aacaccagca acgcgttccc gtggggtcgc ggcggcaccg gcctgcgcca cgtgagcatc
     3661 tacgacagct cgaacttcac tctgcagggc gtcacgacgc cgatcatcat cagccagctc
     3721 cgctaccgcc cgaacacgaa cacgtcgtcg ctcgcatcga actacccgat cgggggcacg
     3781 gtgcagctgt cgacctgccc gatcgaccag agcggagtcg tcgcgggctt gccgctcagc
     3841 acctacgtgg gcgccaacgt cacgacgtgc ttcagcggtc cgatgtcgtg gcccgcagcc
     3901 gccgcgacgg ccggtccgtg cccgtggctg atcacggtcc ccttcagcac gaacttcgtc
     3961 tacgacccga accagggcga cctgacgatc gacacggaca tcccgatcca ggcgttcacg
     4021 ggcacgagcc tgcagctcga cgtgcagtcc acgggctcgc tgagcagccg cgcatacatc
     4081 agcacgggct atccgacggg cgcgacggcg atcacgcaaa accacggcgt ggtgtgcgag
     4141 atcacctacg tgccggcgac gggcctgcac ccacaattca gcgcgacgcc gcgcaccggc
     4201 ccggtcgggc agctcgtcca gttcaccgac cagacctaca cgagtgatcc aggtggcgtc
     4261 acgtcgtggg cttgggacgt ggacggcgac tcggtcaccg actacacgac ccagaacccc
     4321 tcgcacacct acacgaccga gggcaacaag accgtcacgc tgaccgtgac gagcgccatg
     4381 ttcggctcgg cgtcgatcac caagaccaac tacatcgtga tcgacgccgt cgacgcgagc
     4441 ttcacgtcct cggtcctgac ggccacgctc gtggccttca ccgacacgtc gaccggcaat
     4501 ccgaccagct gggcgtggga cttcgagaac gacggcatcg tcgacagcaa cttgcagaac
     4561 ccggcgttct tctatccggc gcccggccag tacacgtgcc ggctgacggt cgcggacgcg
     4621 ttcagcaacg acacgacgac gctgaccttc ggcatcggca tcatcccggt gccggccttc
     4681 ggctcgacgt tcagcggcca gctcacgcgc ggcttctggt tccagtcacc gacgcgcttc
     4741 tcggtcgtct cggccagcgt gcccgacgaa accgccaatg gcacgcagaa cgtcgccatc
     4801 ttccggttgg ccgcagcgcc accggtcttc tcggccaccg ctaccggcgg tctcgagttc
     4861 gtggcgctga gccagccgag tgcgagcccc atcccgtgtg tcgtcagctt cgacgtcggt
     4921 gaatacgtcg gcgtgctcgg cgcctgcggc acggcgacga tgatcaactc gtacgccacg
     4981 ccggcgggtc cgttcgcgtc ctcggtgctc ggcaacccga ccacgctgac gcgcttcggc
     5041 acgcagttca acatcaacac gagcggggcc aaccaggcct actggcagga gccaggcggc
     5101 cagatcagcc gcgtggttct cggcgtcacc gcgtgcgcag cgatccccta tggaacgggc
     5161 tcgccgtcgg gcctcggccc ggcagcgccg tcgatgagag cgagtgcact gccgttcgtc
     5221 ggccagaccg ccgtgcacca agtcacgcag agcgacgccc tcgtgctgca gttgatggtc
     5281 ggtggcttcg gtcgcttgtc cgtcccggtc ccgccatttg gcacgatcct gatcggcagc
     5341 ctcgacctga tcgacgtcat gaacggcggc gcaccggtcg ggccgggcac ctacccgtgg
     5401 tccttcgcga tcccgccgaa cccggtgctc gtcggccaga gcatcaactg gcagaacgcc
     5461 cacctcgtga tcccgaccgg cgaatgggcg atgtcgaacg cgatcgagtg gtggatcaac
     5521 aactagacgc gagctgacca gccggggcga agcgcatcgc ttcgcgctcg gcactggcag
     5581 tgagaacgag gccgctcctc cagacgaggg gcggcctcgt tcgttgttgg cgtgaggtgc
     5641 aaaacggatc agcgcgggag gcgcccgtgc tccctacaat gcgcgcgcga tggacgaaac
     5701 caggctctcg cggcagttcc tgagggtgtt cctcgtcttc ctgatcctca cggcgctgct
     5761 cgcgatcagc atcgtgttgt tcgaggacag gaaccagacc cggtggcgca tcctcggcac
     5821 ttcgagcgtg atctcggtgg ccagcgtcgc cgcgatggcg tgcgccgcct tccgcgagcg
     5881 cggccaactg cgctggctcg gcaatgtggg catgtccctc tgcttcgccg cggccgctgg
     5941 catcgtggtg gcgatctggg acttcgtgca cgacgagacg tacggcaggt tcctcggctg
     6001 cctctcgacc gtggcgggct tcacggcgct cggccagctg ttgtggctga tgccgctcat
     6061 ccagccgcac cgctgggtgc agaaggcgac tacgtacacg atcgccgtgc tcacgatgct
     6121 gatctgcttg cagatcgccg cgcgcatcac agacgccgcg tgggatcgcc tctgggtcgc
     6181 catcgcgatc gtggtgacgc tgcaggtcct cgcgcacccg atcctgtggc agctggggcg
     6241 acgggccggc ggcgccgccc agcgccgtct cctgctgagc cacgagcgcg acgacatctg
     6301 gaccgacggc gcgggtcgcc gctactcggt gcgccccctt ctgggcgaca tggcggcggg
     6361 cgatctggcg ggcgatcagg gctgatcagt ccggcggctt gtccccgtgc acgagcgtca
     6421 tcacggcgct gctgacgagc gcgccgcggc cgtcgtgcac ctcggcctcg aacgtactcg
     6481 cgtcgtcggt gccgcgcagg cgtgtgacgt ggatgtcgag ccgttcgccc acctcgatcc
     6541 gcgagcgcgc gatcgcgaac ttgcggcagg ccacgaccat gccgacgcgc acgccgccgc
     6601 cgtgcaggaa cgcttcgtaa ccgaggcacg cggccacggc ctgcgccatg agctcgagcg
     6661 tgacaacggt gtcgacacca ccaccgcgca ccaggaggcc gccttcgcgc acgacgaggc
     6721 ggacg
//
LOCUS       DASRJA010000684         7164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_375518,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000684 DASRJA010000000
VERSION     DASRJA010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375518"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1407
                     /locus_tag="VFC01_30675"
     CDS             40..1407
                     /locus_tag="VFC01_30675"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HZN84016.1"
                     /translation="MTASYAVVGGGISGLVSAYRLRVAAGPDATITLFDPADRLGGVL
                     RTERIGGQPLDVGAEAFIARRPEVPALLAELGLAGRQIGTTGARPLIYSQRRLHPLPS
                     DTLQGIPAHPQGLAGLVDEATIAWMLDERTRPFSWRPGADPSVAELVGDRFGDQVVTR
                     SVDPLLAGVYAGSAATIGVRSAAPTLAAALDMGARNLTDAVREALPPPLTGSVFGAVD
                     GGYGVLVDELVRRAGIRWAQVAIVQVSRAPHGWDVVDDEGQRWHADAAVLAVPAPRLP
                     KIIEGVAPRTAAAARRIAVASTALVALALPGGTPLPEQSGVLVASGERLRAKAITLSS
                     RKWGRRGNVELVRLSYGRFGDSAETNAARSAGDEDLLEWASQDLATVFGVNVEPVDCH
                     VHRWIDAMPQYGPGHPDLVTDLRAGLPPTLAVAGGYLDGIGVPACVAAATRAAASLIT
                     ARVAR"
     gene            1413..2108
                     /gene="hemQ"
                     /locus_tag="VFC01_30680"
     CDS             1413..2108
                     /gene="hemQ"
                     /locus_tag="VFC01_30680"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HZN84017.1"
                     /translation="MAKLDFDTLNATIRYLMFSVFAVRPGALGDDRAGVVDEMATFLK
                     QQEERGVVVRGVYDVAGMRADADYMFWTHAETLEALQATYSDFRRTTTLGRASTPVWS
                     SVALHRPAEFNKSHIPAFLAGEEPGNYVCVYPFVRSLDWYLLPDDERRRMLSEHGMAA
                     RGYKDVRANTVPAFALGDYEWILAFEAPELHRIVDLMRDLRATDARRHVREETPFFTG
                     PRVGVEELVTKLP"
     gene            2284..3207
                     /locus_tag="VFC01_30685"
     CDS             2284..3207
                     /locus_tag="VFC01_30685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84018.1"
                     /translation="MRNVAWLIGVASAIGFAAWLIGGWGGKSTVEVVDDLGLVGFALF
                     ATVCSGFAALKSSGRQRVAWICFSIGTGGWVVGSALWAYYELWAGVAPFPSLADAGYL
                     VLPVAVCVGLVLFPVGYTGQSQTRLVLDGLIFAGALFEVAWVLVLRTIYETGGASRFA
                     VGLSLAYPVTDLVIITVAVMVLARARTGQRTTLTLLAVGIVSMALSDSAFVYLNAHGV
                     YSTVNLIDLGWAAGLVLIGVAALISCGFAPATDAATPQPPNQVSMWLPYVPTCWPPWF
                     ACRRSSPYPDWAPLSSRPSCWSPSCWCGSSS"
     gene            3168..3902
                     /locus_tag="VFC01_30690"
     CDS             3168..3902
                     /locus_tag="VFC01_30690"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZN84019.1"
                     /translation="MLVTIVLVRQFIVIGENRRLLATVADQALRDPLTGLANRALFND
                     RLTHAVQLHQRDQQSVAVLSLDLDDFKLVNDSMGHPAGDALLVLIAERLMHCVRTSDT
                     IARLGGDEFAVLMEGKPEHARLIAHRVVQAFDDPFDIDGHDLLIRPSVGLAVAAADGP
                     EVSADELLKQADIAMYSAKRSRTGGVHTFTPDMHLIEPGGTRGVRGREWRRRHRRGHG
                     ATARRTSTRDRPARLEPRLPAEVRPS"
     gene            3865..4623
                     /locus_tag="VFC01_30695"
     CDS             3865..4623
                     /locus_tag="VFC01_30695"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN84020.1"
                     /translation="MSLVYQPKFDLRDNDIVGVEALVRWPHHDRGLLGPDHFLPLVRE
                     HGLMRAVTELVLAQALDDVASWHAHGFTVPVAVNIFAPSLGDLELPRQIARALSERNL
                     RPDALTVEITEDLLLDNLARTRTVLDRLRESGVRIAIDDFGSGYSALWYLRDLPVDEV
                     KLDRQFIVPVLVDPRAAAIVRAVIDLAHVLGVTTVAEGIEDALTAIRLLEYGCEVAQG
                     YYYSPPVAAQAMLEMLSSRQQDDHRAASTAVPTT"
     gene            4710..6356
                     /locus_tag="VFC01_30700"
     CDS             4710..6356
                     /locus_tag="VFC01_30700"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HZN84021.1"
                     /translation="MSEAKTGQELLFDALRTKGTAFTREERRKFGLLGLLPTAEKTLD
                     QQVEHCWNEFSTRREGLDRHIYLRALQDRNETLFYRVLRDHIAETMPIVYTPTVGEAC
                     QRFSEIYRRPRGLFVSYPDRADLREVLRNRPHREVDVIVVTDGQRILGLGDQGIGGMG
                     IPIGKLSLYTLIGGIEPARTLPIVLDVGTDNVELLEDPQYLGWRHRRIDDDDYYAFID
                     EFVTAVHEELPDVLLQWEDFATAHALPILDRYRDKLLTFNDDIQGTAAVTLGALHGAA
                     KVAGRPVSQQQVVMLGAGSAGMGVLDMVRREMVAQGLSDADASSRIWVVDIQGLLTDD
                     RTDLTAEQQKFAQPGNRVADWGLSGAAQLADVVHHVDVGVLLGLSTAAGAFTEEIVRD
                     MAGKTERPIIFPLSNPTSRAEAHPAELDQWTDGRALIATGSPFAPLHRDGVERPIAQC
                     NNVYIFPAMGLAVTAAQATRVTDEMMRVAAATLGDASPALGDPNAPLLPAWTDVPDVA
                     VRIAHAVGLQAVADGVAPQRSAEQLTERIAQVRWTAEYRA"
     gene            6648..>7164
                     /locus_tag="VFC01_30705"
     CDS             6648..>7164
                     /locus_tag="VFC01_30705"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZN84022.1"
                     /translation="MPKIAWVTAIATVLAVALTAWMIGGWVSDHKVAVVSVFVILGLL
                     VIAVLVRHFIVVSKMRQLLLMAADQALRDPLTGLANRALFHDRLTHAMQLHQRDNQSV
                     AVLSLDLDDFKMVNDNLGHPAGDALLVLAAERILGCVRASDTVARLGGDEFAVLLEGR
                     AEDSHLVAHRVV"
BASE COUNT         1140 a         2255 c         2442 g         1327 t
ORIGIN      
        1 agccgtgatc accgacgtcg tggcgttggt gcattcgctg tgactgcgtc gtatgccgtt
       61 gtcgggggcg gaatttcggg tctggtgtcg gcataccgcc tgcgcgtcgc ggcggggccg
      121 gacgcgacga tcacgctgtt cgacccggcc gaccggcttg gtggtgtgct gcgcaccgag
      181 cggatcggcg gccagccgct ggacgtcggc gccgaagcgt tcatcgcacg taggcccgag
      241 gtgcctgcac tgctggccga attgggtctg gccggtaggc agatcggcac caccggcgcg
      301 cgaccgctga tctatagcca gcgcaggctg cacccgctac caagcgacac tctgcagggc
      361 atcccggcgc acccgcaggg gctggccgga ttggtcgacg aggccacgat cgcatggatg
      421 ctcgacgaac ggacccgccc gttctcgtgg cggcccggcg cggacccgtc ggtggccgaa
      481 ctcgtcggtg accggtttgg tgatcaggtg gtgacccgct cggtcgatcc actgctcgcg
      541 ggcgtgtacg ccggatcggc ggccaccatc ggggtgcggt cggccgctcc gacgctggca
      601 gcggcgctgg acatgggcgc ccgcaacctg accgatgcgg tgcgtgaggc gctaccaccg
      661 ccgttgaccg ggtcggtttt cggggccgtc gacggcggat atggggtgct ggtggacgag
      721 cttgtccgtc gcgccggcat ccggtgggcg caggtggcca tcgtccaggt cagccgcgcg
      781 ccgcacggct gggacgtagt cgacgacgag gggcagcgct ggcatgcaga cgcggcagtg
      841 ctggccgtcc ccgcgccgcg cctgccgaaa atcattgagg gcgtggcccc ccgcaccgcg
      901 gccgccgcgc gacgcatagc ggtcgcatcc acggcgctgg tggcgctggc gttgccgggc
      961 ggcaccccac tgcctgagca gtcgggtgtc ctggtggcga gcggagaacg gttgcgcgcc
     1021 aaggcaatta ccttgtcaag ccgcaaatgg gggcggcgtg gcaacgtcga gctggtgcgg
     1081 ctgtcgtacg gccggttcgg cgactccgcc gaaaccaacg cagcccgcag tgccggcgac
     1141 gaggacctgc tggagtgggc gtcgcaggac ctggccaccg tgttcggcgt caacgtcgag
     1201 cccgtcgact gccatgtgca ccggtggatc gacgcgatgc cgcagtacgg acccggccat
     1261 cccgacctgg tgaccgatct gcgcgccgga ttgccgccga cgctggccgt agcaggcggc
     1321 tatctggacg gcatcggggt gcccgcctgc gtggccgcgg cgacgagggc ggcggcgtcg
     1381 ctgatcaccg cgcgcgtggc acgatagaag acatggccaa gctcgatttc gacacgctca
     1441 atgcgaccat ccgatacctg atgttttcgg tgttcgccgt ccggccgggg gcgttgggtg
     1501 acgaccgtgc tggggtcgtc gatgagatgg ccactttcct caaacagcag gaggagcgtg
     1561 gcgtcgtggt ccgtggtgtc tacgacgtcg cgggcatgcg cgccgacgcc gattacatgt
     1621 tctggaccca cgccgagacg ctcgaggcgc tgcaggcgac ctattcggat ttccggcgga
     1681 cgaccacgct cgggcgggcc agcacaccgg tgtggagcag cgtcgcactg caccgtccgg
     1741 ccgagttcaa caagagccac atcccggcgt tcctggcggg cgaggagccc ggcaactacg
     1801 tctgcgtgta cccgttcgtc cggtcgctgg actggtacct gctgcccgac gacgaacgcc
     1861 gccggatgct ctctgagcac ggcatggcgg cccgcgggta caaggacgta cgcgccaata
     1921 ccgtgcccgc cttcgcgctc ggcgactacg agtggatcct ggctttcgag gcgcccgagt
     1981 tgcaccgcat cgtcgacctg atgcgcgatc tgcgggccac cgacgcgcgc cgtcacgtgc
     2041 gcgaagaaac accgttcttt acgggcccgc gcgtgggggt cgaagaactt gtcaccaaac
     2101 tcccgtgaca cgtttgctga tttcgcgcgt cgacgtgtga taattgagcg ccgtcctcaa
     2161 gctggcaatg ccattgcgca tgcagctacc acgaaaaact gcgaggcccc gctgttcggc
     2221 aaatactggt gatgagtcgg ggttgcgtgt cgtagagccg cagattttgc tagcgtctga
     2281 tcggtgcgga acgtcgcatg gctcattggt gtcgcctcgg cgatcggatt cgccgcctgg
     2341 ctgatcggtg gctggggcgg taagtcgacg gttgaagtcg tcgacgacct cggcctggtc
     2401 gggtttgccc tgttcgccac cgtgtgctcg ggattcgcgg cgttgaagtc gagtggcagg
     2461 cagcgcgttg cgtggatctg cttcagcatc gggaccggcg gatgggtggt cggttcggcg
     2521 ctgtgggcgt attacgagtt gtgggccggt gtggcgccgt ttccgtcgct ggcggatgcc
     2581 gggtatctgg tcctcccggt ggccgtctgt gtaggcctgg tgctcttccc ggtcggatat
     2641 acgggccaat cgcagacccg gctggtgttg gacgggctga tcttcgcggg cgcgctgttc
     2701 gaggtggctt gggtgctcgt gctgcggacg atctacgaaa cgggcggcgc aagccgtttc
     2761 gccgtaggcc tgtccctggc atatcccgtg acggacctgg tgatcatcac cgtggcggtg
     2821 atggtgctcg ctcgcgcccg aaccggtcag cggaccactc tgacgcttct ggccgtgggc
     2881 attgtgtcga tggcactgtc ggatagcgcc ttcgtctacc tgaacgccca cggcgtctac
     2941 agcaccgtca acctgatcga cttggggtgg gcggcggggc tggtgctgat cggtgtggcc
     3001 gcactgatca gttgtggctt cgcgccagct acggatgcag ctaccccaca gccgccgaac
     3061 caggtgtcga tgtggttgcc gtacgtgcct acctgctggc caccgtggtt tgcgtgccgg
     3121 cgttcatctc cgtacccgga ctgggcccca ttatcttcac ggccttcgtg ttggtcacca
     3181 tcgtgctggt gcggcagttc atcgtgatcg gcgagaaccg acggctgctg gcaacggtgg
     3241 ccgatcaggc gttgcgcgat ccactcaccg gtctggccaa ccgggcactg ttcaacgacc
     3301 ggctgacgca cgcggtgcag ctgcaccaac gcgatcagca atcggtcgcg gtgttgtcgt
     3361 tggacctcga cgacttcaag cttgtcaacg acagcatggg gcatcccgcc ggcgacgcgt
     3421 tgctggtcct gatcgccgag cgattgatgc actgcgtacg gaccagcgac acgatcgcac
     3481 gtctcggcgg tgacgagttc gcggtgttga tggagggcaa gcccgaacac gcgcgactga
     3541 tcgcgcatcg ggtggtccag gcgttcgacg acccgttcga catcgacgga cacgacttgt
     3601 tgatacggcc gagcgtcgga cttgccgtgg ctgcggccga cgggccggag gtatccgccg
     3661 acgagttact gaaacaggct gatatcgcga tgtattcggc gaagcgctcg cggaccggtg
     3721 gtgtgcacac cttcaccccg gacatgcatc tcatcgaacc gggaggaact agaggcgtca
     3781 gagggcggga atggcggcgt cggcaccggc gcggccacgg tgcgactgct cggcgaactt
     3841 cgacgcgcga tcgaccagct cgacttgagc ctcgtttacc agccgaagtt cgaccttcgt
     3901 gacaacgaca ttgtcggtgt cgaggcattg gtccgctggc cgcatcacga tcgcggtttg
     3961 ctcggcccgg accatttcct tcctctggtt cgcgagcacg gactgatgcg cgcggtcacc
     4021 gaacttgtgc tggcccaagc gctcgacgat gtcgcgagct ggcatgcaca tggcttcacc
     4081 gtgccggttg cggtcaacat ctttgcgccg tccctgggag acctggagct gccacgacag
     4141 atcgcgcgtg cgctctccga gcgcaacctg cgacccgatg cgctgaccgt cgagatcacc
     4201 gaggatcttc tgctggataa tctcgcccgc actcgcactg tgctagatcg gttgcgagag
     4261 agcggtgttc gcatcgcgat cgacgacttc ggcagcggct attcggcgct ctggtacctg
     4321 cgtgacctgc ctgtagacga agtgaagctc gacagacaat ttattgtgcc ggtgctcgtc
     4381 gacccgcgcg ccgccgcgat cgttcgtgcg gtcatcgacc tcgctcacgt gctgggggtg
     4441 acgactgtcg ctgagggcat cgaagacgcg ctgacggcga tcagactgct cgagtacggc
     4501 tgcgaggtcg cgcaggggta ttactacagt ccaccggtgg cagcccaggc gatgctcgag
     4561 atgctttcgt cgcgacagca ggacgatcat cgcgcggcat cgaccgcagt gccaacgact
     4621 tgacgcggta gtcgttctgt accggagcgc tgcgggatgg aagagttggt cgcgagaatc
     4681 ccgtaactgc gccggaggaa ggactccagt tgtctgaggc gaagaccgga caggaactgt
     4741 tattcgacgc gttgaggacg aagggcacgg cgtttacccg tgaggagcgc cgcaagttcg
     4801 gcctactggg gctcctgccc actgccgaga agactctcga ccagcaagtc gagcactgct
     4861 ggaacgagtt ctccactcgc cgtgagggtc tcgaccggca tatctacttg cgcgcgttgc
     4921 aggatcgcaa cgagacgctc ttttaccggg tgctgcgcga ccatatcgcc gagacgatgc
     4981 cgatcgtcta caccccaacg gtcggcgaag cctgccagcg cttcagcgaa atctaccggc
     5041 ggccacgggg actgttcgtg tcctatccgg accgggccga cctgcgcgag gtgctccgca
     5101 accgccccca tcgcgaagtc gacgtcatcg tggtgacgga cgggcagcgg atcttgggcc
     5161 ttggcgacca gggcatcggc gggatgggta tacctatcgg caagctgtcg ttgtacaccc
     5221 tcatcggcgg aatcgaaccg gcccgcaccc ttccgatcgt gctcgatgtg ggcaccgaca
     5281 acgttgaatt gctggaagac ccgcagtatc tcggctggcg ccaccgcagg atcgacgacg
     5341 acgactacta cgcgttcatc gacgaattcg tcacggcagt gcacgaggaa ctgccggatg
     5401 tgctgctgca gtgggaggac ttcgccaccg cacacgcgct gccgatcctc gaccgctatc
     5461 gcgacaaatt gctcacgttc aatgacgaca tccagggcac cgctgccgtc accctgggcg
     5521 cgctacatgg cgcggcgaag gtggccggcc ggccagtgtc gcagcaacag gtcgtcatgc
     5581 tcggggcggg ctccgcaggc atgggcgtcc tcgacatggt gcggcgggag atggtggcgc
     5641 agggactgtc cgatgcggac gcatcgtcgc ggatctgggt cgtcgacatc cagggcctgc
     5701 tcaccgacga tcgcacggac ctcaccgccg aacagcagaa gttcgcccag ccgggaaacc
     5761 gcgtcgcgga ctggggcctg tccggagccg cacaactggc cgacgtcgta catcacgtgg
     5821 atgtcggtgt gctgctgggg ctttcgacgg cggccggtgc attcaccgag gagatcgtgc
     5881 gcgacatggc tggcaagacc gaacgaccga ttatctttcc gctctccaac ccgacgagcc
     5941 gcgcagaggc ccatccggcc gaactggacc agtggaccga tgggcgtgcg ctgatcgcga
     6001 cgggttcacc gttcgcaccg ctgcaccgcg acggcgtcga acgcccaatt gcccagtgca
     6061 acaacgtcta catcttcccg gcgatggggc tggcggtcac cgcggcgcag gccacccggg
     6121 tcaccgacga gatgatgcgc gtcgccgccg cgacgctcgg ggacgcctca cccgcgctcg
     6181 gcgatccgaa tgcgccgctg ctgcccgcgt ggacggacgt gccggacgtg gccgtccgca
     6241 tcgctcacgc ggtcggattg caggcggtcg cggacggcgt cgccccccaa cgcagcgccg
     6301 aacaactgac cgagcgcatc gcacaggtgc gctggacggc cgagtatcgc gcctgatgcg
     6361 ggatctgcgg gcccccgacg cgcgccatca tgtccgcgaa gagagccgct cttcccggtc
     6421 agcgggttgg tgccgaagaa cgcgtataca aagtgctgta gacgtttgct gcacccgctt
     6481 cgctgtcctc tactcctttt cgtgactcgt gctgcggggc aacgtccgct atacgaaatc
     6541 acttatcaac gtgtgcgtaa aagcactgat ccgattgctt cggtgagcgg gcttcgggca
     6601 cggcgcttcc tactatgcgt gtcgagaaat gttgctagcc tctgacgatg ccaaagatcg
     6661 cgtgggtcac ggctattgcc actgtgctag ctgttgcgct caccgcgtgg atgatcggtg
     6721 gctgggtcag cgatcacaag gtcgctgtgg tctccgtctt tgtcattctg ggcctgctcg
     6781 tgatcgccgt gttagtgcgg cacttcatcg tggtcagcaa aatgcggcag ctgctgctga
     6841 tggcggccga tcaggcgtta cgcgacccgc tcaccggtct tgcgaatcgt gcgctgttcc
     6901 acgaccgcct cacgcacgca atgcagttgc accaacgcga taaccagtcg gtcgcggtct
     6961 tgtccttgga tctcgatgat ttcaagatgg tcaatgacaa cctcggtcat cctgccggcg
     7021 acgctctgct ggtgctggcc gcggaacgga ttctcggctg cgtgagggcc agtgacacgg
     7081 ttgcccggct gggtggcgac gaattcgccg tgctgctgga ggggcgagca gaagactcac
     7141 atctggttgc gcatcgggtg gtgc
//
LOCUS       DASRJA010000685        33791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3325324,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000685 DASRJA010000000
VERSION     DASRJA010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 33791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33791
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3325324"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VFC01_30710"
     CDS             complement(<1..864)
                     /locus_tag="VFC01_30710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN84023.1"
                     /translation="MHIGDHAIGGAQSPALIVAGGRTVSYGELYARSQRVAALLYDAG
                     LRRGDGVALVLPNRPEFLEITWGCQLSGLYYSAINTHFTSDEVAYIIEDSEAKAVFVD
                     TGADIRISVGGDGRDWSSYEEVLATAGDPPPISDGSEMLYSSGTTGRPKAVRRPLPED
                     GRGSWAQKVLEYTLTQRYGMSTSSVYLSPAPLYHAAGVNYTMAVQRVGAASIVMPRFD
                     ADEVLRLIETYRVTHAQFVPTMFVRMLKLPEAVRERYDVSSLQCVIHAAAPCPVDVKH
                     RMMKWFGPIIHE"
     gene            complement(872..1738)
                     /locus_tag="VFC01_30715"
     CDS             complement(872..1738)
                     /locus_tag="VFC01_30715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN84024.1"
                     /translation="MDLNETRERIIYEKNGPVAKITLNWPEKANAQDQKQVEEIDDAL
                     RDADRDYDIKVLVLKANGPGFCSGHAIGNNAVDYPEFVEGFKATGMPWKPQTDLFVKP
                     ILNLWEFSKPTISQVHGYCVGGGTHYGLVTDIVIASEDAYFSYPPLQGFGMPSGECSI
                     EPWVFMNWRRAAYYLFLAEVIDAKKALEVGLVNEVVKREELDARVDAIARHIAQAPLT
                     TLLATKANLKRAWELMGMRVHWQSSNDLVALASVSKDVQELIQQVFKDKILPSEMAKR
                     QAAASTDGSSAT"
     gene            complement(1747..1983)
                     /locus_tag="VFC01_30720"
     CDS             complement(1747..1983)
                     /locus_tag="VFC01_30720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZN84025.1"
                     /translation="MGDFLIRIPRVSVAVSEAELVELLVTDGQHVEEGTPIYVIATEK
                     AEQEIEAGASGTVQWTGEVGTTYDIGVEIGVIKT"
     gene            complement(1976..2974)
                     /locus_tag="VFC01_30725"
     CDS             complement(1976..2974)
                     /locus_tag="VFC01_30725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZN84026.1"
                     /translation="MDEKEMTMREALHLALDQAMEADDRVFLLGEDIADPGASGPTAG
                     LSTKYGHDRVLDTPISEAAIVGAAIGAAIDGLLPVAEIMIMDFIGIAADQLINNAAKL
                     RFMTAGRTTAPITVRTQVYGGLATGATHSQTLEAWFMHIPGMKVIVPSTPRDGKGLLT
                     SAIFDEDPCLFVETIRLQGQKGLVPTDPGFAIPLGHAEIKRSGTDVSIISYGRCVLES
                     LAAADALQEQGVSAEVVDLRTLVPLDIDTIVESVRRTRRAVVVHDAVQFAGPGAEIAA
                     TLQSECFSELTAPIERVGARFVPTPAAPTLEAQLYPSSARISAAAQRTLEKAGARG"
     gene            complement(2986..3966)
                     /locus_tag="VFC01_30730"
     CDS             complement(2986..3966)
                     /locus_tag="VFC01_30730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZN84027.1"
                     /translation="MTYPADVQRRMYALMVLMKAADDRLSKGIGTGEFMCVYWPSRGQ
                     EAIAAAMGVTLRRDDQLVTTYRGLHDLIGKGVPLEEIYGEMMGRTVGASRGKGGTMHI
                     AKPEVGVMLSTGIVGAGPPVGVGLAMAAKRKGLDRVTVVSFGDGATNTGSFHEAANMA
                     ALWALPMVFVCQNNQYAEMTPTADTMKLETVAERAAGYGMPGVRVDGNDPLAVATALD
                     EALSRARRGDGPTFVECVTFRFRGHYFGDRMPYIPKEQLAEAMAADPVPRFRRQLIDT
                     GVCSDGELNGIDGGALIDVEAALKTVLSAEAPSIDELDRDVYATPISYPV"
     gene            complement(3963..4283)
                     /locus_tag="VFC01_30735"
     CDS             complement(3963..4283)
                     /locus_tag="VFC01_30735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84028.1"
                     /translation="MPKGIMYVETMPVSPDKEAEYHKWYNETHLAQIVSVEGIVSARR
                     FAPTDGKGPFIAIYELDCDDLDAAVHRLGELGASGNMTGLENLAMDPKPIPRVYREIG
                     SYES"
     gene            complement(4286..5152)
                     /locus_tag="VFC01_30740"
     CDS             complement(4286..5152)
                     /locus_tag="VFC01_30740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZN84029.1"
                     /translation="MGRVTGKVALISGAARGQGRSHARLLAAEGADIIAVDICEDIET
                     NEYPLARPEDLDETARLVEKEGQRAYTEIADVRDRAALSAAIDRGVAEFGHLDIVVAN
                     AGICPMTAGLPPQAFVDAVDVDLVGVMNLIHASMKHLHAGASIIATGSVAAFMATAVN
                     TSGMDGGPGGAGYAFAKQVVAHYVNDLARTLAPEQIRVNAVHPTNVNTDMLHSPPMYR
                     AFRPDLQNPTREDAEVVFPVIQAMPIPYVEPEDISEAVVFLASDAARYVTGQQLRVDA
                     GGFLKIKPWSGV"
     gene            complement(5166..6305)
                     /locus_tag="VFC01_30745"
     CDS             complement(5166..6305)
                     /locus_tag="VFC01_30745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020108407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZN84030.1"
                     /translation="MSKSASRSAVVGVGYSKILRDKGIDGRPLAVEASRAAIADAGLR
                     VRDVDAVFEYAGDEQAMDIARLVGIENMVVYGDFTPRGPSGLAAAMGAHMAVVSGTCE
                     VALVVRSVTREWGQMSGRALPKAAGPWQWEFPYGAAGGIIPVMGMKKQRRMATLGTKE
                     EEYGWISLNGRRWSSLNERAVMRDILSMDDYLGSRYVSEPLRLLDCDYPVTGACAVVI
                     TTAERAADAQKKPVWIDAIAMGTGWKPDFTYGDDFLFGGTRPCADTMWKRSSVTAADV
                     DVAELYDGFTHLAMAWVEAMGFCGIGEFGDWVDGGNTIGPGGALPLNTSGGQLAEGRL
                     HGLGFLAEGVAQLRGECGQRQVPDAQVAAIANGFGPQCGSLVLTV"
     gene            6421..7563
                     /locus_tag="VFC01_30750"
     CDS             6421..7563
                     /locus_tag="VFC01_30750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN84031.1"
                     /translation="MDFSYPPEVEQFRKELRAWLAANLTEEVVAAGRRRGRDPDTFEM
                     LRAWDAAVADAGWGAVSWPQEYGGRGATVLEQLAYAEETIHARAPVPLNVIGMNNIAP
                     AIMQYGSDAQKRELLPRMVRADDIWCQGMSAPEAGSDLASLRTRAVLDGDEYVVNGQK
                     IWTSLGHRADWCQLYVRTDPDAPKHKGISCLIVDMALPGIEARPLVTLNGDADFAEVF
                     FNDVRVPADALLGPLNGGWQVATTTLSHERAGAARLYAQLEVQLHDLVADLAEANLLD
                     DAATLQRLGEVAVRIKYLEVLCQRSISATLHGGDALGSASLAKSVWAEVGQELATLAF
                     DVLGPLGGHRRWLDRRLTSRSLTIAGGTTQINKSVTATRVLGLPRR"
     gene            7560..8624
                     /locus_tag="VFC01_30755"
     CDS             7560..8624
                     /locus_tag="VFC01_30755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN84032.1"
                     /translation="MNLELTDEQLALRDTVRRFLTEKASVTTHVRPMLDDQTGSTREV
                     WSGLTALGATGVLVPQEHGGLGLTMVEAGVVAEELGAALYPGPWLSSAVAATRALTRT
                     GSEAGSLLAGIADGSTIVTVGALSGERPEVENGILRGKISALPDAVAADVVLVLADDS
                     GDTGLFTVDAISAGVSVMERTHVDPTRKLFDVTFDDVAAPRLASPSADDIQALIDDVL
                     IAYAADALGAATAVMNMAVEYAKVRRQFDQPIGSFQAVQHLCVDMYETVELARSGVLH
                     ALWAADAAPPAERHLAAVRAKAFAGQLATVADTAIQVFGGIGYTWEHDAHLYLKRLLG
                     WSTFLGGSDRYLQEVGKALL"
     gene            8621..9841
                     /locus_tag="VFC01_30760"
     CDS             8621..9841
                     /locus_tag="VFC01_30760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZN84033.1"
                     /translation="MTQVLRGVRVIELASWTYVPSAGAALADWGADVIKVEDVKGGDP
                     GRALVIGGFTRENARANVDFILEIGNRGKRSIAIDIKSDTGREYFGRLLATADVFLTN
                     WLPAALERARLTVDEIRAFNPNIIIARGTGTGVRGPDANKGGFDAATYLSRGGVSYTM
                     TPFGTETPAVQGPGFGDLQGGITLAGGVCAALFHRERTGEPSVVDSSLLAQAMWTIAP
                     SISVADLFDIDGIPGAPPGLAINPLVNRYKTRDNRWIQLVFLQPDRFWAGFCERIGLP
                     ELASDERFVPSSNLIANATEACALVGEAIASQDLDHWRKALADEPGVWAALATPKETL
                     NDPQVTPNGYTVANVDDKGSEYQIVAAPVQFNETAPPPARAPEHGQHTEEILLELGLD
                     WDDISAAKDSSAIL"
     gene            9851..10684
                     /locus_tag="VFC01_30765"
     CDS             9851..10684
                     /locus_tag="VFC01_30765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN84034.1"
                     /translation="MEQRTLESVIYEREPPIARIILNKADRANTKDAQLVQEVDACLH
                     EADRDKDIKVVILKANGKGFCGGHVARWGPDENPYPDFGDTFEDLYKGTADLFLWPTL
                     YLWEFPKPTISAIHGYCMGGGIYLGLLTDFSVASEDAYFQMPLAQSLGEPGGHTMIEP
                     WLMMNWHRTMDWLLLAPTLSAQEALEWGLLNKVVPRDELEATVEEMARKIGQIPLTTL
                     MAVKNNVKRAWELMGMRVHLQVSHILTNMVGAASDVQARRAELMQSGLKPRDFVEGEQ
                     T"
     gene            complement(10741..12414)
                     /locus_tag="VFC01_30770"
     CDS             complement(10741..12414)
                     /locus_tag="VFC01_30770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZN84035.1"
                     /translation="MVNWDKSVDLLIAGSGGGGMVAALAALDSGIEPLVVEKQAVIGG
                     ATGMSGGMVWLPNNPLMVAEGIPDSHEDGLAYFDDVVGDIGAASSPERREMFLTAGNE
                     MIDFLVGKGVRIARCPGYSDYYPNHKGGNAAGRSIEGIPYDAAALGDWSDKLQRMDKN
                     WNRGFVVKTSELVIVQYFNRSPRAFAVAMRVFLRTRVARLRRRDILTNGASLIGQMLK
                     SLIDQRGEPPIWTNTAMDDLIVEDGRVVGARLTRDGTSLNVQARKGVLLAAGGFSRNA
                     EMRRRYSGDQPNEAQWSLANPGDTGEVLQTAMRLGAKTDLLDEAWWLPTVFVADPRAR
                     AIGSGRQRPGAIYVDGTGKRFCNESNSYVEVGKAMYANKAVPCWQVFDEGYLGRYVSG
                     ANPFQKRTIPEAVIEQGAVKRADTIAELARQIDVPAQALEQTVQRFNGFAVKGLDPDF
                     GRGQSAYNICLGDPGYKPNAALGPIDHAPFYATQVYPADVGTCGGVITNEHAQVLDEQ
                     DRVIDGLYATGNITATVMGRTYLGAGGSIANTMIFGYVAARHAAGCGGQ"
     gene            complement(12408..13757)
                     /locus_tag="VFC01_30775"
     CDS             complement(12408..13757)
                     /locus_tag="VFC01_30775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZN84036.1"
                     /translation="MNWTPLPVPWAVQAVDRIPKQRYYDKEFYALEAELFWPRVWQMA
                     CRLEEIPSRGDFVEYEILDQSVIVVRLDDDNVRAFYNSCRHRGMKIVEGCGSRRNFVC
                     PFHGWCWGLDGANTFVLRPEIFDESNLAADDLSLAPVRCELWGGCAWINLDDSAPPLR
                     ECIEPFASRHDEWKVGALRTEWWKSCLLPVNWKLATEAFMEGYHVPQTHPQLLPSSHT
                     PDPSIHPIIETSLHFMRTLGSGMGGMTHENDVRIAEGLQHMSLPDDPTEAMAAWRGAV
                     NDAVTKWHRARDCDFPDLNDLDRRRVIDPIGFCFPHYFLLPQYSSASSYRIRPLGPEE
                     TLFEIWSLTRYPADRSPGRPTPPEPLAPDDPSWPTIPAQDFSNLPRQQKGLHAKGFEY
                     MRLSNQVEGLISNFERVIDGFLSGVPYERLVPAIQRTNTTIDVPVADLGLSERAPAW"
     gene            13821..14063
                     /locus_tag="VFC01_30780"
     CDS             13821..14063
                     /locus_tag="VFC01_30780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HZN84037.1"
                     /translation="MDTWIDDLARALGEEPLTAAETTELLDAARDVAHRVERKWTPLS
                     TFMVGCAVGRAGGADRGEMLSATLRKLASLLPPEES"
     gene            14195..14869
                     /locus_tag="VFC01_30785"
     CDS             14195..14869
                     /locus_tag="VFC01_30785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN84038.1"
                     /translation="MIRRPADERPRVGREETREALIEATAQIMLEEGYAAATSRRVAA
                     KAGVKPALVHYYFPSMDDLFVAVLRDKAENNLVRQRQAIAEAKPLHALWLLNSATDAQ
                     LFTEFLAMANHRKAIRSEISAYAMRFRDLEEGVVTLALKARGVDLELFPPVVMSMIMG
                     GLARMVLHEQGLGITHGHEEATAFIERCLERFEMPLWDEKQPGSAQLSDPARADGKEV
                     TGNTIA"
     gene            14983..15411
                     /locus_tag="VFC01_30790"
     CDS             14983..15411
                     /locus_tag="VFC01_30790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmpS family transport accessory protein"
                     /protein_id="HZN84039.1"
                     /translation="MLRFFKRIWVLLVILLVVVIAAFVVDRFRGFFGHTVLTKPGAGL
                     VNDPEPFNPKVVKYEIFGTGGVATINYLDLDAQPQKAENAPLPWELTLTTTAPAASPN
                     IIAQSDGGSITCRISVDGVIKDERTTDGVNAQTFCLVKSA"
     gene            15408..18317
                     /locus_tag="VFC01_30795"
     CDS             15408..18317
                     /locus_tag="VFC01_30795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN84040.1"
                     /translation="MSNTHVGAHPMIPRFIRLFSVPIFLGWIGIVVLVNVIAPQLEKV
                     GEAHAVSLAPNDAPSLEAMQRIGKTFQEFDSNSSAMVVLEGDQPLGDAAHKYYDGLID
                     KLEADKKHVEHIQDFWGDPLTASGAQSADGKAAYVQVYMAGNQGESLANESVQAVRDI
                     VDETPPPPGVKAYVTGPAPLTNDQHHAGDKSIQLITMITIAVIFVMLLFVYRSIVTVL
                     LVLVMMFIELAAARGIVAALGYYDLIGLSTFAVNLLTTLAIAAGTDYAIFLVGRYHEA
                     RENGEDRETAFYTMYHGTAHVILGSGLTIAGATFCLHFTRLPYFQTLGIPLAIGMLVA
                     VAAALTMAPALLTICSRFGLFDPKRAMKTRGWRRVGTAVVRWPGPILAASVALALVGL
                     LALPSYQPSYNDRNYLPKDIPANQGYSAADRHFPQARMNPEMLLVETDHDVRNSADML
                     VIDRIAKGIFHIPGVGRVQSITRPEGTPIEHTSIPFLISMQGTTQQMNQDYMQKVMAN
                     MLKQANDMQTSIDTMERMQGITVQMAATTHSMVEKMKNMTVDVAELRDNIANFDDFFR
                     PLRNYFYWEPHCYDIPICWSLRSIFDTLDGIDTMTDDIQAIVPDMEKLDTLMPQMVAL
                     MPSMIETMKSMQHITLTMYQSQKSQQDQMQAMQENQGAMGEAFDASKNDDSFYLPPEV
                     FDNADFKRGMKMFVSPDGKAVRFIISHESDPATPEGIKLIPQIKNAAFEAIKGTPLEG
                     SKIYLAGTASTYKDMQEGSNWDLLIAGISSLCLIFIIMLLLTRAVIASAVIVGTVLLS
                     LGTSFGLSVLIWEHILGQPLHWMILAMSVIILLAVGSDYNLLLVSRFKEEINAGIKTG
                     IIRSMAGTGAVVTSAGLVFAFTMMSMVVSDLVVVGQVGTTIGLGLLFDTLVIRSFMTP
                     SIAAMMGKWFWWPQNVRNRPRPVPWPKPPRHSTETPVSVGVGASNVDDNPTGPIR"
     gene            18325..18663
                     /locus_tag="VFC01_30800"
     CDS             18325..18663
                     /locus_tag="VFC01_30800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN84041.1"
                     /translation="MKRTRLASIAAAAIAPALVFAAPAHADPDTDFGKELHTYGIYGQ
                     KDYNAWIAKIACKRLYNGVDQDADKSAKFIFLQLQKGSTTEQAWQFLGAALRTYCPEK
                     LPILDAAAQQ"
     gene            18674..19114
                     /locus_tag="VFC01_30805"
     CDS             18674..19114
                     /locus_tag="VFC01_30805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5078 domain-containing protein"
                     /protein_id="HZN84042.1"
                     /translation="MLIKASVAAAAVSVAAIAVPAVASADATDDYPIPNRILKTTCTV
                     DQYMAAVRDSDPVYYERYMIDYNNKSPEVQQAARDRIYWFFSLDYAGRRQYSEDTATN
                     AFYEQMAWNWPNWAKLFFNNKGVAAHGTDTCAQYPPSDPAVWNW"
     gene            19111..19749
                     /locus_tag="VFC01_30810"
     CDS             19111..19749
                     /locus_tag="VFC01_30810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004571502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN84043.1"
                     /translation="MTPAPRRTLNDAVTQFWSIAAPLYDLPVLQHWVYRPAQDEVIAV
                     LRDRGARKVADIACGTGILAARIADELDAGEVYGVDMSDGMLAQARARSTEVQWRKGP
                     AEHLPFEEDTLDAVVTTSAFHFFDQPAALREFHRVVAPGGLAAVATLSPRTPLRLHRL
                     SANRLNPAHNPSPEEMRELFEGAGFRVHDQHRVRRPAWTRVLSDLITIGIKP"
     gene            complement(19754..21277)
                     /locus_tag="VFC01_30815"
     CDS             complement(19754..21277)
                     /locus_tag="VFC01_30815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84044.1"
                     /translation="MTSTSFGWVLLDGQGPDAATLDHDTFDVQTGDDGASGDTSQHAA
                     AVRGAQAIATASGHQVGTVHVTWTDEVEANAAALMKSLSDLGFHNVHPVSLRNAAQAW
                     GTELGRQSEHTSTGLCILEPDAATIMVVPTEAGTVRTAITDTRETTEDLVESLRTVFR
                     KDGWLPESLYLVGAHTDLDDVARPILEALPIPVSDSVDTQLALARGAALTTVSEVDAD
                     SPRTEPTPEPPWRVSVTKESTTSAAETVMAPAVSPVDTDSTQAIETSRTEPPRSGQRS
                     LVLDAKKLSLMAAAVAGLGAALSLTAGSALNVENTSMRAAEPAASGASVTSASVHPVP
                     APTPTPQAPPVQPLAAEPPPPPPLPPEMLVPPAEPLAVAVPEPAPAVVPQQTVTVPTA
                     VPVVPAPVVAPAPAEHLVAPVAPPPAAPVVAPPAPPPAAPLAAPAAPLPAEALVAPVA
                     PPPAAPVVMPPAAGPAPGPPQAPVAPPAAPPVPPEPTEPPPQDPMTVVSNLLFGSLP"
     gene            21907..23277
                     /locus_tag="VFC01_30820"
     CDS             21907..23277
                     /locus_tag="VFC01_30820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84045.1"
                     /translation="MRIEIPTDEIACWWEETKISTHLRLAAGICLLTTGLLIGSAGGA
                     IATADTESTGSTSQSQGADPSTQSVNTASAPTASSTEPATTKTPQNAVRTALLGVLQK
                     IRTLGKVQQQPVKKPVADPATADSKPDGTEGDVGAAVTEPVAQSDVVASDSNATEPET
                     YGAASESTDPAPAIDPVPPVPLGPPPVVPASNVLEPVTNAIARVASAALAVPFVLASL
                     PTSPTPVADVIAAVQNILIAVNDAIAPIVQLPSDFVSLLVAAGMSASPVDSIGNSAGA
                     RLSAATGTAPVLPFMPMVPPVPPNSPVGVMPLLGDVTAPLTLGGLAAAGLSADLSLSG
                     TAPLTADGAGPTGALSLLEHTVRAFLAPASLSALAALALPGVAGLLIVCAAGMRVGYR
                     QAKAALAVRTTGISRFARQGPLGVVRSGSLVTLHPRSSGPQHSRPRRVVRPDVSRAAP
                     LLEQVA"
     gene            23296..24783
                     /locus_tag="VFC01_30825"
     CDS             23296..24783
                     /locus_tag="VFC01_30825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003914553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN84046.1"
                     /translation="MTREISRQAFVRGALGGVVAGALLGSCRSTAPEPPASPSAASPS
                     PPASVAGPQDWSALDEAIDGLVVLPSSADYAAAKSVFNARFDDSTPAAVVVVKSVDDV
                     QRAAEFAARNNVKIAARSGGHSYIGASTASGAMIIDLRQLAGEITYDDGRGLATISAA
                     VQLDSVQTALAAHGRSLPSGSCPTVGVAGLTLGGGLGSDARRSGLTCDALVSASVVLP
                     SGEAITASADDHADLFWALRGGGGGNFGVVTSFTFRTFPIADRDVVTLVFPEGSTEQA
                     ILGWHDWLHDADRAIWGMVNITVGTDAGGCTVVLATPAGDGRSGASDLSSVIGVRPVS
                     NTSRTLSRMDFVHYFEGGAQAARPRAFVAGSDIIGAMTSAAAESIVAATSAWPPDAGS
                     ATAVIESLSGAVRDVAADDTAFPWRRQAACIQWYTEPPSQAAIETADKWLAGAHAAVH
                     AHSVGGYVNYVEPDTPAARYFAGNLDRLTAIGRKYDPGGLMNSGM"
     gene            24814..25461
                     /locus_tag="VFC01_30830"
     CDS             24814..25461
                     /locus_tag="VFC01_30830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN84047.1"
                     /translation="MNTPGSFRITRSVGAAAAAAWALPIAFIEPPSAAADPCPDVEVV
                     FARGTTEPPGVGGVGQAFVDSLRSQLGARSVGVYAVDYPATRDFVRSAPAGARDASTH
                     IQAMASNCPNIRMVLGGYSQGAGVIDMSTTAMPPQVADHVAAAALFGAPRSAFADSLS
                     PGPLPAIGPLYAAKTIDLCVPNDPICSNGWDMRAHGAYVETGMVNQAATFAASRL"
     gene            25827..26717
                     /locus_tag="VFC01_30835"
     CDS             25827..26717
                     /locus_tag="VFC01_30835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN84048.1"
                     /translation="MSPRHIAVSIGAAAVATSVLQAAPIPLAQAAPCPDAEVVFARGT
                     TEAPGPGPTGDAFIDSLRSRVAGKSVGVYAVDYPATTDFPTAVEGISDARTHILATAA
                     SCPKTKMVLGGFSQGAAVMGFVTASVVPDGVSATEVPAPMPPEVANHIAAVALFGKPS
                     TRFMRAINDPDVTIGPQYVGKTIDLCVDNDLVCDMTGRSFSAHNQYVEAGIVDQGATF
                     VANQLQASWAADAAVAPTEGATPSPQTPPAPLPLSAAAGAAAPPAGIAGPTPHLPTGP
                     VPPGPPATPVAAPPPAGPVI"
     gene            27131..28105
                     /locus_tag="VFC01_30840"
     CDS             27131..28105
                     /locus_tag="VFC01_30840"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HZN84049.1"
                     /translation="MTQLKSRICAFVVALISVFSMLSVPVAAAEAANITDIQHVNDRW
                     DKVSVYSPSMNTVIVNDVFKAPKSGAPVFYLLPGIDGGDDIAGGGIASGGTSWFGMTD
                     IQGFFGNKNVNVVSPLGGAFSWFTNWVADPGKQYQTYMTQELPPLINKQYNTNGRNAV
                     GGLSSTGGTAIDYAVQAPGLYRAVGSYSGLLNPADQPQQVSMTLMGGGQSADAMWGPA
                     GGPLWVAHDPSKNVAKLKGVAVYAAASASGGVGTVDRLPEDFGPNFTGGLIERIVADS
                     TKVFADSAAAAGVPINYVVRPDGSHTWGLFESEMQESWNTTVGPALGA"
     gene            complement(28109..29434)
                     /locus_tag="VFC01_30845"
     CDS             complement(28109..29434)
                     /locus_tag="VFC01_30845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZN84050.1"
                     /translation="MPLTTPAPDARTAADNTPASRDRAVDVARLAALVVVMFGHCALL
                     LATIDSGGLRIGNLLGELPAIAPITWIVQVMPLFFLAGGAAGAYGWHTGTPWGTWLFT
                     RSQRLCRPVFWYLAAWSVGLLVARMTLGAESAAGLGRECVALLWFLGVYLVVLAFVPA
                     LTRLRTGVGVAVLVASLITAAVAIDGIRIAVGTSTAGSANFVIVWLIPVVIGVAYARR
                     LIGPRAALAAAAAAFVAQVVLAMVGPYEISLVVTGMERMSNVSPPTLLLALHCTWMSC
                     AFVATAGAVSRWAARPRIWRLVVVGNGGAMTLYLWHVPAIAVATFALNAVGLDAYDVD
                     APGFWALLALRAVVFAAVMAVAFRLLSPLEHRRLPWWDAPVGATGAHATAAGALICVS
                     GVALLLLAKHGLSDFAGWTELGCFVIAAGAARLSASVPAKNSTHQAVDG"
     gene            29538..30320
                     /locus_tag="VFC01_30850"
     CDS             29538..30320
                     /locus_tag="VFC01_30850"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008126 - acetylesterase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HZN84051.1"
                     /translation="MRILNVAVLVAAVAVFGSSGVAAAAPKDYCADLKGGIAGATCVI
                     QLSDAGYNVDISFPTDYPDQKSVADYITQTRDGFLNVAKSGAPRDTPYELNITSTNYG
                     SAIPPRGTQALVLKVYQNVGAAHPQTMYQAFNWDQSYRKPIVWDTAGDDKKTPLWRVD
                     DPLKTVAPIVQAELQKQMAPPVPAVPPAQPGQPTATPPPAPTAPPVAIAPTALYDPMN
                     YQNFAVTNDGVIFFFSQGQLLPEAAGATQVLVPRAAIDPMLA"
     gene            30341..31045
                     /locus_tag="VFC01_30855"
     CDS             30341..31045
                     /locus_tag="VFC01_30855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015627608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HZN84052.1"
                     /translation="MPRRVGHDSKVQLVGRNLLANGRGRWLALAASVVVAAGMFYAQD
                     TKRPCCAENAAVAPTSQAPAPLPAAAPSVASPTEAELLAASAPVTAGQDFQFALPAGI
                     APEEGLQIHTIWVARAISVLFPEIKTIGGYRQDALKWHPNGLAIDVMIPNYHSDEGIE
                     LGNQIAGFALANAKRWGVIHVIWRQGFYPGIGAPSWTADYGNETANHFDHVHIATDGG
                     GYPTGKETYYIGSMKL"
     gene            31375..31689
                     /locus_tag="VFC01_30860"
     CDS             31375..31689
                     /locus_tag="VFC01_30860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84053.1"
                     /translation="MTVVSFIAIHPKPGGNWEELQKQLKKGSDLARKHGAENVTALWN
                     VAGGTATGTVILLSSCADWTSYGKIQDAMLADPEMQALMADPNSPIARWDTYVSQTIP
                     DI"
     gene            complement(31760..32080)
                     /locus_tag="VFC01_30865"
     CDS             complement(31760..32080)
                     /locus_tag="VFC01_30865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84054.1"
                     /translation="MTTSTTRKVTGFKYAATVLGAFAVLAIAPATAYAVDNDPAPGGG
                     GCTYTDADGYDIPIDNGQDVFVDGKIVSCRDGKIVVTTAPKRNVVRPPVLKGGTLPVL
                     TKQP"
     gene            complement(32240..>33791)
                     /locus_tag="VFC01_30870"
     CDS             complement(32240..>33791)
                     /locus_tag="VFC01_30870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZN84055.1"
                     /translation="QAQAIAIAERQFLAMVNHPGIVKIYNFVEHPGFDGRPIGYIVME
                     YLGGTTLQAILAKQRAEAPQGEPNPMMPVEQALGYVLEVMPALSYLHSLGLVYNDLKP
                     ENIMLTEDNVELIDMGAVSGIGDFGYIYGTKGFQAPEIVRTGPTVATDIYTVGRTLAK
                     LTVDMPDDPYAETLPEPDDVPLFQRYESFYRLLVRATNPRPAQRFSSADEMATQCKGV
                     LREVLAEQTGVPRPGTSVLFTMHGSEFGADLAPQRTDVFVDGRLHPVRLTARDVARAL
                     PTPAPTEDRESDWRMDWDDGISALDSGNLRAALKCFQRVVAAVPGEAAPKVASAATAE
                     LMLGTEDARNAERLRQSCERYYRTLWRTDHSMVSAAFGLARQLAARGDRPVAIDVLDE
                     VPLTSRHYGEAQLTSVLMLLDGRPIAEITEADLRDSARRVGQLSETEPRALQVRALVL
                     GTALDWLRSGATPAATDPILSQVFDQEGLRTGLEEALRELARHSPRRRHRYTLVDLAN
                     SIRPPSWM"
BASE COUNT         6022 a        10987 c        10858 g         5924 t
ORIGIN      
        1 ctcgtggatg atcgggccga accacttcat catgcggtgc ttgacgtcga ccgggcaggg
       61 cgccgccgcg tggatcacac actggaggct ggacacgtca tagcgctccc tgacggcctc
      121 gggcagcttg agcatccgca cgaacatcgt tgggacgaac tgcgcatgcg ttacccggta
      181 agtttcgatc aggcgcagca cctcgtcggc gtcaaatctc ggcatcacga tcgacgccgc
      241 gccgacccgc tgcaccgcca tcgtgtaatt gaccccagcc gcgtgataga gcggcgccgg
      301 cgacaagtac acgctcgagg tgctcatgcc gtaccgctga gtcagcgtgt actccaacac
      361 cttctgcgcc catgaccccc tcccgtcctc cgggagaggc cttcggaccg ccttgggccg
      421 accggtggtc ccggacgagt agagcatctc ggaaccgtcg gagatcggcg gaggatcccc
      481 tgcggtggcc agcacttcct cgtaggagct ccaatcccgc ccgtcgccac ctacactgat
      541 gcggatgtca gcgcctgtgt cgacgaacac cgccttggcc tcggagtctt cgatgatata
      601 ggcgacctcg tctgaggtga agtgggtgtt gatcgcgctg tagtagagcc cggaaagctg
      661 gcagccccag gtgatttcga ggaactcggg ccggttcggg agcaccagcg caaccccgtc
      721 gccgcgccgc agtcccgcgt cgtacagaag cgcggcaacc cgttggcttc tcgcgtataa
      781 ctcgccgtac gaaacggtcc ggccacccgc gacgatcaag gccggtgact gggcccctcc
      841 gatcgcgtgg tcgccaatat gcacggcgga cctaggtggc tgacgagccg tcggtcgatg
      901 cggcggcctg acgtttcgcc atctccgacg gcagaatctt gtccttgaac acctgctgga
      961 tcagctcctg cacgtccttg ctgaccgatg cgagcgccac gaggtcgtta gagctctgcc
     1021 agtgcacccg catgcccatc agctcccacg cgcgcttgag attggccttg gtcgccaaca
     1081 gtgtcgtcag cggtgcctgg gcgatgtggc gcgcgattgc gtcgacccgc gcgtcgagtt
     1141 cctcacgctt gaccacctcg ttcaccagcc cgacttccaa cgccttcttg gcgtcgatga
     1201 cctcggcgag gaacaggtaa taggccgcgc ggcgccagtt catgaagacc cacggctcga
     1261 tcgagcactc gcctgatggc atgccgaatc cctgcagcgg cgggtaggag aagtaggcat
     1321 cctcggaggc gatcacgatg tcggtgacga gaccgtagtg agtgccacct cccacgcagt
     1381 atccgtggac ttgagagatc gtcggcttgg agaactccca caggttcagg atcggcttga
     1441 cgaacagatc ggtctgcggc ttccacggca tgcccgtggc cttgaatccc tcgacgaact
     1501 ccggatagtc caccgcgttg ttgccgatgg cgtggcctga gcagaacccg ggcccattgg
     1561 ccttgaggac cagaaccttg atgtcgtagt cgcggtcagc gtcccgcagc gcgtcatcga
     1621 tctcctcgac ttgcttctgg tcctgggcat ttgccttctc gggccagttc agcgtgatct
     1681 tcgcgacggg accgttcttt tcgtagatga tccgttcgcg ggtctcgttg agatccattc
     1741 cctctcctag gttttgatga cgccgatctc gacgccgatg tcatacgtgg tgccgacttc
     1801 gccggtccat tggacggtgc ccgatgcacc ggcctcgatc tcctgttccg ccttttccgt
     1861 tgcgatcacg tatatcggcg tgccctcctc gacatgctga ccgtccgtca cgagcaactc
     1921 gacaagctcg gcctcggata cggctaccga tacccgcggg atgcggatga gaaagtcacc
     1981 cacgcgcgcc ggccttttcg agcgttcgtt gcgcggccgc ggagattcgc gcggatgacg
     2041 ggtagagctg cgcttccaga gtcggggctg ctggcgtcgg gacaaacctg gctccgacgc
     2101 gctcgatggg agcggtcaac tcgctgaaac actcggactg cagcgtcgcc gcgatttcgg
     2161 ctcccgggcc ggcgaattgg accgcgtcat gcaccacgac ggcccggcgg gtgcgtcgca
     2221 ccgattcgac gatggtgtcg atgtcgagcg gaacgagtgt gcgcagatcg accacctcgg
     2281 cgctgacacc ctgctcctgc agcgcgtcgg cagcagcaag cgattcgagc acgcagcggc
     2341 cgtagctgat gatgctgacg tcggtacccg accgcttgat ctcggcgtgg ccgagcggaa
     2401 tagcgaaccc gggatcggtt ggcacaagac ctttttggcc ctgcaatcgg atcgtttcga
     2461 cgaacaagca gggatcctcg tcgaagatcg ccgacgtcag caggcccttg ccgtcgcgcg
     2521 gggtggacgg cacgatgacc ttcatcccgg gaatgtgcat gaaccaggcc tcaagcgtct
     2581 gcgaatgcgt tgcgcccgtt gccaatcccc catacacctg ggtgcggacg gtgatgggcg
     2641 ccgtcgtccg gcccgcggtc atgaagcgca gcttggcggc gttgttgatc aactggtcgg
     2701 ctgcgatgcc gatgaagtcc atgatcatga tctcggccac cggcagcagg ccgtcgatcg
     2761 ctgcaccgat ggcggcaccg acgatcgcgg cctccgagat cggtgtgtcc agcacgcggt
     2821 cgtggccgta tttcgtcgac agccccgcgg tcgggccgga cgcaccgggg tcggcgatgt
     2881 cctcaccgag caggaaaacc cggtcgtcgg cctccatcgc ctggtcgagc gcgaggtgca
     2941 gcgcctcacg catcgtcatc tctttctcgt ccatgcgcgt cgtcctcaga ccgggtagga
     3001 gatcggggtc gcgtacacgt ctcggtcgag ctcatcgatc gacggcgcct cggcgctcag
     3061 caccgtcttc agtgccgcct caacgtcgat gagcgcacct ccgtcgattc cgttcagctc
     3121 gccgtcgctg cacacgccgg tgtcgatcag ctgacggcgg aaccgcggta cggggtcggc
     3181 cgccatcgct tctgccagtt gctctttggg gatgtagggc atccggtcgc cgaagtagtg
     3241 gccgcggaag cgaaacgtca cgcattcgac gaacgtgggg ccgtcgccac ggcgtgcccg
     3301 cgacaacgct tcgtccagtg ccgtcgctac ggccagcgga tcgtttccgt ccacacggac
     3361 acccggcatg ccgtagccgg ccgcccgctc ggcgaccgtc tcgagcttca tcgtatccgc
     3421 ggtcggcgtc atctcggcgt attggttgtt ctgacataca aacaccatgg gcagggccca
     3481 cagcgcagcc atgttcgccg cctcgtggaa tgagccggtg ttggttgcgc cgtcgccgaa
     3541 gctgaccacg gtgacccggt cgagcccctt gcgtttggcg gccatcgcca gcccgacacc
     3601 caccggcggt cccgcgccga cgatcccggt cgacagcatc acgcccacct cgggtttagc
     3661 gatgtgcatg gtcccgccct tgccgcggct cgcgccaacg gtgcggccca tcatctcgcc
     3721 gtagatctct tccagcggaa cgcctttgcc gatgagatcg tgcagtccgc ggtaggtggt
     3781 caccagctgg tcgtcgcgcc gcaacgtgac tcccattgcg gcggcgatcg cctcctgccc
     3841 gcgcgacggc cagtacacgc acatgaactc gccggtgccg atgcccttgg acagtcggtc
     3901 gtcagcggcc ttcatcaaca ccatcaacgc gtacatccgc cgctgaacgt ccgccgggta
     3961 cgtcatgatt cgtacgaccc gatctcgcgg tacacgcggg gaatcggctt cggatccatg
     4021 gcgaggtttt ccaggccggt catgttgccg ctggcgccga gctcgcccaa ccgatgcacg
     4081 gccgcatcga ggtcatcgca gtcgagttcg tagatcgcga tgaacggacc cttgccgtcc
     4141 gtcggcgcga agcgacgggc cgagacgatc ccctcgaccg acacgatttg cgccagatgg
     4201 gtttcgttgt accacttgtg gtactcggct tccttgtccg gcgagaccgg catggtctcc
     4261 acgtacatga tccccttggg catgctcaca cccccgacca cggcttgatc ttcaagaagc
     4321 cacccgcgtc gacccggagc tgttggccgg tgacgtagcg ggccgcgtcg ctggcgagaa
     4381 acaccaccgc ctcactgatg tcctccggtt cgacataggg aatcggcatt gcctgaatca
     4441 ccgggaacac cacctccgcg tcctcgcgag tcggattctg caagtcgggt cggaaggcgc
     4501 gatacatagg gggactgtgc aacatgtcgg tgtttacatt cgtcgggtgg accgcattca
     4561 cgcggatctg ctcgggagcc agagtccgcg cgaggtcgtt gacatagtgt gcgacaacct
     4621 gtttggcgaa cgcatacccc gcaccgccgg gtccgccgtc catcccgctg gtgttgaccg
     4681 ctgtcgccat gaaggcggcg accgagcccg tcgcgataat cgacgcgccg gcatgtaggt
     4741 gcttcatgct ggcgtgaatg agattcatta cgccgaccaa atccacgtcg acggcgtcga
     4801 cgaacgcctg cggcggcagt ccggcggtca tcgggcagat gccggcattc gcgaccacga
     4861 tgtccagatg gccgaactcg gcaacacccc gatcgatcgc cgcggacagc gcagcacggt
     4921 cacggacgtc ggcgatctcg gtgtatgcgc gctggccctc cttctccacc aaccgggccg
     4981 tctcatcgag gtcctcgggc cgcgcgagcg gatactcgtt ggtctcgatg tcttcgcaga
     5041 tgtccacggc gatgatgtcg gcgccctcgg ctgcgagcag ccgcgcgtgt gatcgtccct
     5101 gaccgcgcgc ggctccgctg atcagcgcca ccttgcccgt caccctgccc atcgccggtc
     5161 cttcctcaca ccgtcaacac caacgacccg cactggggtc cgaaaccgtt cgctatggcc
     5221 gcgacctggg cgtcgggcac ctgacgctgg ccgcactcgc cacgcaattg ggcaaccccc
     5281 tcggccagga acccgaggcc gtgcagccgg ccttctgcca gttggccgcc gctggtgttc
     5341 agcggcaacg caccgccggg cccgatcgtg ttgccaccgt cgacccaatc gccgaactcg
     5401 ccgatcccgc agaagcccat cgcctccacc caggccatcg cgagatgagt gaacccgtcg
     5461 tacagctccg cgacgtcgac gtcggccgcg gtcaccgacg agcgcttcca catggtgtcg
     5521 gcgcacggtc gcgtgccgcc gaacaagaag tcgtcgccgt aggtgaagtc gggtttccac
     5581 ccggtcccca tcgcgatggc gtcgatccac accggtttct tctgcgcgtc ggccgcccgc
     5641 tccgcggtgg tgatgacgac ggcgcaggca ccggtgaccg gatagtcgca gtcgagcaac
     5701 cgcagtggtt ccgagacgta gcgggagccg aggtagtcat ccatggacaa gatgtcccgc
     5761 atgactgcgc gttcgttgag cgacgaccat cgtcggccgt tcagcgaaat ccagccgtac
     5821 tcttcttctt tcgtgccgag cgtggccatc cgccgctgct tcttcatccc catcaccggg
     5881 atgatgccgc cggccgcccc gtatgggaac tcccattgcc atgggcccgc tgccttgggc
     5941 agcgcccgcc cgctcatctg gccccactcg cgggtcaccg agcgcacaac caaggccact
     6001 tcgcacgtcc ctgacaccac cgccatgtgc gcgcccatcg ccgcggccaa acccgagggg
     6061 ccgcggggtg tgaaatcgcc gtacaccacc atgttctcga taccgaccag gcgggcgatg
     6121 tccatggcct gctcgtcgcc tgcgtattcg aacaccgcgt cgacgtcgcg cacccgcaaa
     6181 ccggcgtcgg caatcgcggc ccgcgaggcc tcgacagcaa gcggccgccc gtcaatgccc
     6241 ttatctcgga ggatcttcga gtaaccgaca cccacgacgg ctgaccggct ggccgacttg
     6301 ctcactcaga agcaccatcc acgacgttgt gcggcgatat tgcagccaac tctaactgca
     6361 gcggtagcta tactacctaa aattataaag aaaccatcgg accgatcctg acaggacacc
     6421 gtggacttct catacccgcc ggaggtcgag cagttccgca aggagctgcg cgcgtggttg
     6481 gccgcgaacc tgaccgagga ggtggtggcg gccgggcggc ggcgcggccg tgaccccgat
     6541 acattcgaga tgctgcgtgc gtgggacgct gcggtggccg acgccggctg gggtgccgtc
     6601 tcctggcccc aggagtacgg cgggcgcggg gccaccgtgc tcgaacaact cgcctacgca
     6661 gaggaaacca tccacgcgcg agcgccggtg ccgctcaacg tgatcgggat gaacaacatc
     6721 gcgccggcga tcatgcagta cggcagcgat gcccagaagc gcgagctgct ccctcgcatg
     6781 gtgcgcgccg acgacatctg gtgccagggc atgtcggcgc cggaggctgg ctccgacctc
     6841 gcctcgctgc gtacccgtgc ggtgttggac ggcgacgagt acgtggtcaa cgggcagaag
     6901 atctggacgt cgctcggaca tcgcgccgat tggtgtcagc tctacgtgcg caccgatccg
     6961 gacgccccca agcacaaggg catctcgtgc ctgatcgtcg acatggcgtt gcccggtatc
     7021 gaggcgcgcc cgctcgtgac gctgaacggc gacgccgact tcgccgaggt gttcttcaac
     7081 gatgtgcggg tgcccgccga cgcgttactg ggcccgctga acggtgggtg gcaggtcgcg
     7141 acgaccacgc tgagccatga gcgcgccgga gcggcccggc tgtatgcgca gctagaggtg
     7201 caattgcacg atctggtggc cgatctggcc gaggccaatc tgctcgacga cgcggcaacc
     7261 ctgcaacggc tgggcgaggt ggcggtgcgc atcaaatatc tggaggtgct ctgccaacga
     7321 tcgatctcag cgacgctgca cggcggcgac gcgctggggt cggcgagcct ggccaagagc
     7381 gtgtgggccg aagtcggcca ggaactcgcc accctggcct ttgacgtgct cggcccgctc
     7441 ggtggtcatc ggcgatggtt ggaccgtcgg ctcacgtcgc gctcgctgac catcgcgggc
     7501 ggcaccaccc agatcaacaa gagcgtcacc gcgacgcgag tgttagggct gccgcggcga
     7561 tgaacctgga gctcaccgac gaacagctgg ccctgcgtga cactgtgcgc cgctttctga
     7621 ctgaaaaagc aagtgtgaca acccatgtgc ggccgatgct cgatgatcag accggcagca
     7681 cccgtgaggt gtggagtggg ttgaccgcgc ttggggccac cggagttctg gtcccgcaag
     7741 aacacggagg cttgggcctg acgatggtcg aggcgggggt tgtcgccgag gaactcggtg
     7801 ccgcactgta tccggggccg tggctatcga gcgcggtcgc cgcgacgcgc gcactgacgc
     7861 ggacgggtag cgaagccggc tcactgctgg ccggtatcgc cgacggatcc accatcgtca
     7921 cggtcggcgc gctaagcggt gagcgtcctg aggtcgagaa cgggattctt cgcggcaaga
     7981 tctcggcgtt gccggacgcg gtcgctgccg acgtcgtgct ggtcctggca gacgacagcg
     8041 gggatacagg actgttcacc gtcgacgcga tttcggcggg cgtgtccgtg atggagcgga
     8101 cccacgtgga tccgacgcgc aagctattcg acgtcacgtt cgacgatgta gccgcgccgc
     8161 ggctcgcctc cccgtcggcg gatgacatcc aggcgttgat cgacgacgtg ctgatcgcct
     8221 acgcggccga tgcgctgggc gcggcgacag cggtgatgaa tatggccgtc gaatacgcca
     8281 aggtgcgccg gcagttcgac cagcccattg ggagttttca ggcggtgcag cacctctgtg
     8341 tcgacatgta cgagacggtc gaactcgcgc gcagcggggt gctgcacgcg ctgtgggcgg
     8401 ctgacgccgc accacccgcg gagcgccatc tggccgcggt gcgcgccaag gcattcgcgg
     8461 ggcaactggc gacggtcgcc gacaccgcga tccaggtctt cggcggcatc ggctacacgt
     8521 gggagcacga cgcacacctg tatctcaagc gcttgctcgg ctggagcacc ttcctcgggg
     8581 gctccgaccg atatctacaa gaggtcggaa aggcgttgct atgacacaag tgctgcgcgg
     8641 agtgcgcgtc atcgaactcg catcgtggac gtacgtgccg tctgccggtg cggcgcttgc
     8701 ggattggggt gcagacgtca tcaaggtgga ggacgtgaag ggcggcgatc ccggccgcgc
     8761 gctggtgatc ggcgggttca cccgggaaaa cgcccgcgcc aacgtcgatt tcatcctcga
     8821 gataggaaac cgcgggaagc gcagcatcgc gatcgacatc aagtccgaca ccggccgcga
     8881 gtacttcgga cgcctgctgg ccaccgcgga cgtgttcctg actaactggc tacctgccgc
     8941 actggagcga gcgcggctca cggtcgacga gatacgggcg ttcaacccga acatcatcat
     9001 tgctcgcggc accggtaccg gggtccgcgg gcccgatgcc aacaagggcg gattcgacgc
     9061 ggccacctat ctatcccgcg gcggcgtgtc gtacaccatg acgccgttcg gcaccgagac
     9121 gccggccgtg cagggtcccg gtttcggcga tctgcaaggc ggcatcacgc tggccggcgg
     9181 tgtatgcgcc gcactcttcc accgggaacg caccggcgag ccctcggtcg tcgactcttc
     9241 tctactggcg caggcgatgt ggacgatcgc gccgtcgatt tcggtggccg acctgttcga
     9301 tatcgacggc ataccgggtg caccgcccgg tctggcgatc aacccactcg tgaatcgcta
     9361 caagacgcgg gacaacaggt ggatccagtt ggtgttcctg cagccggatc gcttctgggc
     9421 gggcttctgc gagcggatcg gcttgccgga actcgcatcc gacgaacgct tcgtgccgtc
     9481 gagcaatctc atcgccaacg ccaccgaggc gtgtgcgctc gtcggggagg cgatcgcaag
     9541 ccaagacctc gaccattggc gcaaggcgct cgcagacgaa cccggtgtct gggctgcgct
     9601 cgcgacgccg aaggagacgc tgaacgaccc ccaagtcacg ccgaacggct ataccgtcgc
     9661 caatgtggac gacaaaggca gcgagtacca gatcgtggcc gcaccagtgc aattcaatga
     9721 aacggctccg ccgccggcac gcgctcccga acatggacag cacaccgagg agatcctgtt
     9781 ggagctcggc ctcgattggg acgacatctc ggcggccaag gacagcagcg cgattctgta
     9841 ggagaagacc atggagcagc gcaccctcga atcggtgatc tacgagcgtg aaccgccgat
     9901 cgcgcggatc attctgaata aggccgatag ggcgaatacc aaggacgcgc agctggtcca
     9961 agaagtcgac gcctgcctgc acgaggccga tcgcgacaag gacatcaagg tcgtcatcct
    10021 caaggccaac gggaagggct tctgcggcgg gcacgtcgcg cgctggggcc cggacgaaaa
    10081 cccctatccg gatttcgggg acaccttcga ggacctgtac aagggcactg ccgacttgtt
    10141 tctgtggccg acgctgtatc tgtgggagtt ccccaagccc accatctctg cgatccatgg
    10201 ctattgcatg ggcggcggca tctacctcgg cctgttgacg gatttctccg tcgcttcgga
    10261 ggacgcgtac ttccagatgc cgttggcgca gagcctcggc gagcccggcg ggcacacgat
    10321 gatcgagccg tggctgatga tgaattggca tcgcaccatg gactggttgc ttttggcgcc
    10381 aacgctttcc gcgcaggagg ctctggagtg ggggctactg aacaaggtgg tgccgcgcga
    10441 cgagctcgag gcaaccgtcg aggagatggc ccgcaagatc ggacagatcc cgttgacaac
    10501 gctgatggcg gtcaagaaca acgtcaagcg ggcctgggag ttgatgggga tgcgcgtgca
    10561 ccttcaagtg agccacatcc tcaccaacat ggtcggcgcg gcctcggacg tgcaggcgcg
    10621 ccgcgcagag ctcatgcaat caggtttgaa gccaagagat ttcgtcgagg gcgagcagac
    10681 gtaaaactgt ccaaataccc cagaaaatgg gtactttagc gtctgctcgc gctaggaaag
    10741 ttattgcccg ccgcagcctg cggcgtggcg ggcggcaacg tagccgaaga tcatcgtgtt
    10801 agcgatgctg ccgccggcgc ccagatacgt gcggcccata accgtggccg tgatgttgcc
    10861 cgtcgcatag agaccgtcga tcacccgatc ctgttcgtcg agcacctgtg cgtgctcgtt
    10921 ggtgatcact cccccgcatg tgccgacgtc ggcggggtac acctgggtgg cgtagaacgg
    10981 cgcatggtcg atcgggccga gcgcggcatt cggcttgtat ccggggtcac ccaagcagat
    11041 gttgtacgcc gactggccgc gtccgaagtc ggggtcgagg cccttgaccg cgaaaccgtt
    11101 gaagcgctgg accgtctgct ccaacgcttg tgcggggaca tcgatctggc gggccagctc
    11161 ggcgatcgtg tcggcacgct tgacggcgcc ctgctcgatc accgcttcgg ggatagtgcg
    11221 cttctggaac gggtttgcgc ccgagacgta tctgccgaga tagccctcgt cgaagacctg
    11281 ccagcacggc acggccttgt tggcgtacat cgccttgccg acttcgacat acgagttcga
    11341 ctcgttgcag aaccgcttgc cggttccgtc gacgtagatg gcccccggcc gctgccgccc
    11401 cgagccgatg gcgcgggcgc gggggtctgc gacgaaaacc gttgggagcc accaggcctc
    11461 gtccagtaag tcggtcttcg cgcctagccg catcgccgtc tgcagcactt caccggtgtc
    11521 gccggggttc gcgagcgacc actgtgcctc attgggctgg tcgccgctgt accgacggcg
    11581 catttccgcg ttgcgactga agcctcccgc tgcgagcagg acgcccttac gcgcctggac
    11641 gttgagggag gtgccgtccc tagtgagtcg ggcgccgacg acgcgaccgt cctcgacaat
    11701 gagatcgtcc atcgcggtgt tcgtccagat tggcggctca ccgcgctgat cgatcaggct
    11761 cttcagcatc tgaccgatga gtgacgcacc gttggtcagg atgtcgcgac gccggagtcg
    11821 ggcaaccctg gtgcgcagga agacccgcat ggccactgcg aacgctcgtg gtgagcggtt
    11881 gaagtactgc acgatgacca gctcgctggt cttcaccacg aatccgcggt tccaattctt
    11941 gtccatccgc tggagcttgt cgctccagtc ccccaaggct gccgcgtcgt acgggatgcc
    12001 ctcgatcgaa cgacccgctg cgttgccgcc cttgtgattc gggtagtaat cgctatagcc
    12061 ggggcatcgc gcgatgcgaa cgcctttacc gacgaggaag tcgatcatct cgttgccggc
    12121 ggttaggaac atctcgcgac gttccggcga ggacgccgca ccgatgtcgc cgacgacgtc
    12181 gtcgaagtac gccaggccgt cctcatgcga atcgggtatg ccttcggcaa ccatcagcgg
    12241 gttgttcggc agccacacca tgccgccgga catcccggtc gccccgccga taacggcctg
    12301 tttctcgact accaggggtt cgatccccga gtcgagagcc gccagcgcag cgaccattcc
    12361 gccgccgccg cttcccgcga tgagcaggtc gacggacttg tcccaattca ccatgctggg
    12421 gccctctcgg aaagcccgag atcggcgacg ggtacgtcga tggtggtgtt cgtgcgctgg
    12481 atcgcgggca ccaggcgctc gtacggcacg ccggacagaa agccgtcgat gacccgctcg
    12541 aagttggaga tcaggccttc gacctggttc gacagccgca tgtattcgaa ccctttggcg
    12601 tgcaagccct tctgctgacg cggcaggttg gagaagtcct gcgccggaat ggtcggccag
    12661 ctcgggtcat cgggggccag cggctcgggc ggcgtgggcc gtcctggcga gcggtcagcg
    12721 ggatagcggg tgagcgacca gatctcgaag agcgtctctt cgggcccgag cggccggatc
    12781 cggtacgacg acgcgctgct gtattgcggc agaaggaagt agtgcgggaa gcagaacccg
    12841 atcgggtcga tgacgcggcg acgatcgagg tcattcaggt ccgggaagtc gcagtcgcgt
    12901 gcccggtgcc acttcgtgac cgcgtcgttg accgcgccgc gccaagccgc catcgcctcg
    12961 gtgggatcgt cagggagcga catgtgctgc agcccctcgg cgatccggac gtcgttctcg
    13021 tgggtcatcc cacccatgcc ggacccgagg gtccgcatga agtgcaggct ggtctcgatg
    13081 atgggatgga ttgacgggtc cggggtgtgc gacgacggca gaagttgcgg gtgcgtctgc
    13141 gggacgtggt agccctccat gaaggcttcg gtcgccagct tccagttgac cgggagcaga
    13201 cacgacttcc accactcggt gcgcagcgcg cccaccttcc attcgtcgtg ccgggacgcg
    13261 aacggctcga tgcactcgcg caatggcggt gcgctgtcgt ccaggttgat ccacgcgcag
    13321 ccgccccaga gctcacaacg caccggggca aggctgagat catcggctgc gaggttgctt
    13381 tcgtcgaaga tctcgggtcg caagacgaat gtgttggcgc cgtcgagacc ccaacaccac
    13441 ccatggaatg ggcagacaaa gttgcgacga gatccgcaac cttccacgat cttcatcccg
    13501 cgatggcgac aagagttata gaaggcgcga acattgtcgt cgtcgagccg aacgacgatg
    13561 actgactggt cgaggatctc gtactcgacg aagtccccgc gcgacggaat ctcttcgagt
    13621 cggcacgcca tctgccacac gcggggccag aacaactcgg cctcgagcgc gtagaactct
    13681 ttgtcgtagt agcgctgttt ggggatccgg tccacagcct ggaccgccca cggaacggga
    13741 agcggagtcc agttcacgag ttcgtatctt agcaaaaata gcaacttcgc cccgggcatt
    13801 cgtgcgcgaa tactagagcg atggacacct ggatcgatga ccttgcccgc gcccttggcg
    13861 aggaaccact gaccgctgcc gagacgaccg agctgctgga cgcggcccgc gatgtcgcgc
    13921 atcgagtcga gcgcaagtgg actccgctct ccacgttcat ggtcggctgc gccgtcggac
    13981 gcgcgggcgg ggcggaccgc ggcgagatgc tcagcgcgac attgcggaag ctggcatcac
    14041 tgctcccgcc ggaagagtct taggccgacg ggatcagcgg agaccggcac acgttggcga
    14101 ccggatccca accgtgggct aatcgcccgg gcgactgcgc cgacgaattg gcgtagcggt
    14161 tgttgggcgg tcgcccaacc gttagtctgc cttcatgatc aggcgacccg ctgacgagcg
    14221 gccgcgagtc ggccgggagg aaacgcggga ggcgctgatc gaggcgaccg cccaaatcat
    14281 gctcgaagag ggttatgcgg cggcgacgtc gcggcgggtg gcggccaagg ccggcgtcaa
    14341 gcccgcgctg gtgcactact acttcccgag catggacgac ctgttcgtcg ccgtgctgcg
    14401 cgacaaggcc gagaacaatc tggtacgcca gcgccaggcg atcgccgagg cgaaaccgct
    14461 gcacgcgctg tggctgctca acagtgcgac cgacgcccag ctgttcaccg agttcttggc
    14521 gatggccaac catcgcaagg ccatccgcag cgagatctcg gcatacgcaa tgcgtttccg
    14581 cgatctggaa gagggcgtgg tgaccctggc gttgaaggcc cgcggtgtcg acctcgagct
    14641 gtttccgccg gtggtcatgt cgatgatcat gggcggcctg gcccgcatgg tgctccacga
    14701 gcaaggactc ggcatcaccc acggacacga ggaagccacc gccttcatcg agcggtgcct
    14761 cgagcgcttc gaaatgccgt tgtgggacga aaagcagccc ggatcggccc agctcagtga
    14821 cccagcccgc gccgacggca aggaggtgac cggcaacaca atcgcttgag atattccgtt
    14881 tcgcgatgag aagttatacc ggctaagtta tagccgcgat tacgtaagac aggcagagaa
    14941 gagggcgtac atgcgtaccg agggtcgggg gcgaatcgtc tgatgttgcg tttcttcaag
    15001 cgcatttggg tactgctggt gatcctgctg gtggtggtca tagcggcgtt tgtggtggac
    15061 cgcttccgtg gattcttcgg tcacaccgtg ctcaccaagc cgggcgcggg cctggtcaat
    15121 gaccccgaac cgttcaaccc caaggtggtt aagtacgaga tcttcggtac ggggggcgtg
    15181 gcgaccatca actatctcga tctcgacgca caaccgcaga aggccgagaa cgcgccgttg
    15241 ccgtgggagc tgaccttgac gacgacggcg cccgccgcca gcccgaacat cattgcccag
    15301 agcgacggag gcagcatcac ctgccggatc agcgtcgacg gcgtgatcaa agacgagagg
    15361 accaccgacg gcgtgaacgc ccagaccttc tgcttggtga agtcggcatg agcaacacac
    15421 acgtcggggc gcacccgatg atcccgcgct ttatccgcct gttctcagtg ccgattttcc
    15481 tgggctggat tgggatcgtt gtgctcgtca acgtcatcgc ccctcagttg gagaaggtcg
    15541 gcgaggcaca cgcggtgtca ttggcgccca acgacgcacc gtcgctggag gcgatgcaac
    15601 gcatcggtaa gactttccag gagttcgact ccaacagctc ggcgatggtc gtgctcgagg
    15661 gcgaccaacc tctgggcgat gccgcgcata agtattacga cggcctgatc gacaagttgg
    15721 aggccgacaa gaagcacgtc gaacacatcc aggacttctg gggcgatccg cttaccgcct
    15781 cgggtgcgca gagcgcagac ggcaaggccg cctacgtcca ggtctacatg gcgggcaacc
    15841 agggcgaaag cctggcgaac gagtccgtgc aagcagtccg tgacatcgtg gacgagaccc
    15901 caccgccacc cggggtcaag gcttatgtca ccggtcctgc gccgctcacc aacgaccagc
    15961 accacgccgg cgacaagagc attcagctca tcacgatgat caccatcgcc gtcatcttcg
    16021 tgatgctgct gttcgtctac cgatccatcg tcaccgtcct actcgtcttg gtgatgatgt
    16081 tcatcgagct ggcggccgcc cgcggaatcg ttgccgccct tggctattac gacttgatcg
    16141 gcttgtcgac gttcgccgtc aacctgctca ccacgctggc gatagccgca ggaacggact
    16201 atgcgatctt cctcgtcggc cgctatcacg aggcgcggga gaacggcgag gaccgcgaaa
    16261 ccgccttcta caccatgtat cacgggacgg cccacgtcat tttggggtcg ggcctgacca
    16321 tcgcgggtgc gacattctgc ctgcacttca cccgactgcc ctacttccaa acgctgggca
    16381 ttccattggc gatcggcatg ctcgtcgctg tcgcggcggc gctgacgatg gcgcccgcat
    16441 tgttaaccat ctgcagccgc ttcggcctgt ttgatccgaa gcgggcgatg aaaactcgcg
    16501 gttggcgccg tgtaggtacg gccgttgtgc gttggccggg accgatcctt gcggcgtcgg
    16561 tggctttggc gctggtcggt ctgctggcgc ttccgtctta ccaacccagc tataacgacc
    16621 gtaattatct gcccaaggac atcccggcca atcagggtta ctcggcggcc gaccggcact
    16681 tcccgcaggc ccggatgaat ccggagatgc tgctggtcga gaccgaccat gatgtgcgca
    16741 actcagccga tatgttggtc atcgaccgaa ttgccaaggg catcttccac attcctggcg
    16801 tcggcagggt gcagtccatc acccggccgg agggcactcc gatcgaacac acgtcgatcc
    16861 cattcctgat cagcatgcag ggcaccaccc agcagatgaa ccaggactac atgcagaagg
    16921 tcatggccaa catgctcaag caggccaacg acatgcagac gtccatcgac acgatggaaa
    16981 gaatgcaagg catcacggtg cagatggccg ccaccacgca cagcatggtc gagaagatga
    17041 agaacatgac cgtcgacgtg gctgaattac gcgacaacat cgccaatttc gatgacttct
    17101 tccgtccgct gcgcaactac ttctactggg aaccgcactg ctacgacatc ccgatctgct
    17161 ggtcgctgcg gtcgatcttc gacactctcg atggcatcga cacgatgacc gatgacatcc
    17221 aggcgatcgt gccagacatg gaaaagctgg acacgctcat gcctcagatg gtcgcgttga
    17281 tgccgtcgat gatcgagacg atgaagagca tgcagcacat cacgctgacg atgtaccaga
    17341 gccagaagag tcagcaggac cagatgcagg ccatgcagga gaaccagggc gccatgggcg
    17401 aggcgttcga tgcatccaag aacgacgatt cgttctatct gccaccggaa gtcttcgaca
    17461 acgcggactt caagcgtggc atgaagatgt tcgtgtcacc ggacggaaaa gccgtgcggt
    17521 tcatcatctc tcacgagagt gatcccgcga cccccgaagg catcaagctc atcccgcaaa
    17581 tcaagaatgc cgcgttcgag gccatcaagg gcacgccgtt ggagggctcg aagatttatc
    17641 tggcgggtac cgcgtccacc tacaaggaca tgcaggaggg ttcgaactgg gaccttctga
    17701 tcgccggaat atcctcgctc tgcttgattt tcatcatcat gctgttgctc actcgagcgg
    17761 tgatcgcgtc ggcggtcatc gtcggcacgg tgctgttgtc gctgggcacg tcgttcgggt
    17821 tatccgtgct gatctgggag cacatcctcg gccagccgtt gcactggatg atcctggcga
    17881 tgtcggtgat catcctgctg gcggtgggtt ccgactacaa cctgttgttg gtatccaggt
    17941 tcaaagagga gatcaacgcc ggaatcaaga cgggcatcat ccggtccatg gccggcaccg
    18001 gtgcggtggt gacatctgcc ggtctggttt tcgcgttcac gatgatgtcg atggtggtca
    18061 gtgacctcgt ggtggtcggc caggtcggca caacgatcgg tctcggcctg ttgttcgaca
    18121 cgctggttat tcgctcgttc atgacgccgt ccatcgccgc catgatgggc aagtggttct
    18181 ggtggccgca gaacgtgcga aaccggccga ggccggtgcc atggcccaaa ccgccacggc
    18241 actccaccga gactccggtc agcgtcggcg ttggcgcgtc taatgttgac gacaacccga
    18301 caggcccgat cagataggta tgtcatgaag cgaacaagac tggcctcgat cgccgccgcc
    18361 gcgattgcac ccgcgctggt gttcgccgcg ccggcgcacg ccgaccccga taccgacttc
    18421 ggcaaggaac tacacacgta cggcatctac ggccagaagg actacaacgc gtggatcgca
    18481 aagatcgcgt gtaagcggct ctacaacgga gtcgatcagg atgccgacaa gtcggcgaaa
    18541 ttcattttcc tccaactgca gaagggcagc accacggaac aggcgtggca gttcctcggc
    18601 gccgcgctgc gtacctactg ccccgaaaag ctgccgatcc tcgacgccgc ggcgcaacag
    18661 taaggagtca gatatgttga tcaaagcaag tgtcgcggcg gccgccgtga gtgttgccgc
    18721 gattgccgtt cccgccgtcg cctccgcgga cgcgaccgac gactatccga tcccgaatcg
    18781 gatactcaag accacgtgca ccgtcgacca gtacatggcg gccgtacgcg acagcgaccc
    18841 ggtgtactac gagcgctaca tgatcgacta caacaacaag tcccctgaag ttcagcaggc
    18901 cgcccgcgac cgaatctact ggttcttctc gctggactat gcggggcgcc ggcaatattc
    18961 ggaggacacg gcgaccaacg ccttctacga gcagatggcg tggaactggc cgaactgggc
    19021 gaagctgttc ttcaacaaca agggtgtcgc cgcgcacggc accgacacct gtgcccagta
    19081 cccgccgagc gacccggccg tctggaactg gtgacgcccg cgccgcgccg aactctgaac
    19141 gacgcggtca cgcagttctg gagtatcgct gcgccactct atgacctgcc ggttcttcag
    19201 cattgggtgt atcgaccggc acaggacgag gtgatcgcgg tgttgaggga ccgcggtgcc
    19261 cgcaaggttg ccgatatcgc ttgtggcaca gggattctcg ctgcgcgaat cgcggacgaa
    19321 ttggacgctg gcgaggtcta tggcgtcgac atgtccgacg gtatgctcgc ccaggcacgg
    19381 gcgcgctcca cagaggtgca gtggcgcaag ggacctgccg agcatctgcc attcgaggaa
    19441 gacacgttgg acgcggtcgt gacgacctcg gcgttccact ttttcgacca acctgccgcc
    19501 ctgcgtgagt tccaccgcgt agtggctccc ggcggtctcg cagcggtagc gaccttgagt
    19561 ccacgaactc cgctccgcct gcatcggctg tccgccaatc ggctcaaccc cgcacacaac
    19621 ccgtcacccg aggagatgcg ggaactgttc gaaggcgccg gttttcgcgt ccacgatcaa
    19681 caccgcgtcc gacgacccgc ctggacgcgg gtgctgtcgg acctgatcac catcggcatc
    19741 aagccctgac ggattagggc aagctaccga acagcaggtt cgagacgacc gtcatggggt
    19801 cctgcggagg cggttcggtg ggttccggtg gcacgggcgg cgcggccggt ggtgcgactg
    19861 gtgcctgcgg aggtccgggt gccgggccag cggcaggagg catcacaacg ggtgcggcgg
    19921 gtggcggagc aaccggcgcg acaagggctt cggctggcaa cggagcagcc ggggcagcaa
    19981 gaggtgcggc gggtggcgga gcaggcggcg cgacaacggg tgccgcgggt ggcggagcaa
    20041 ccggcgcaac aaggtgttcg gctggcgccg gcgcgacaac gggtgccgga acaacgggca
    20101 ccgcggtcgg aacggtaacc gtctgttgcg gaaccacggc gggcgctggt tcaggcactg
    20161 cgacggcaag gggctcggcg ggaggcacga gcatttccgg cggcaatggt ggcgggggtg
    20221 gcggctcggc ggccaacggt tggacgggcg gggcttgcgg cgtcggagtt ggcgcgggaa
    20281 ccggatggac gctggcagac gtcaccgaag cacccgaggc agctggctct gcggcccgca
    20341 tcgaggtgtt ttcgacgttc agcgccgaac ccgccgtgag cgacagtgcg gccccgagac
    20401 ccgccacggc ggcggccatg aggctcagct tcttcgcatc caacaccagc gaccgctggc
    20461 cgcttcgcgg cggctccgtg cgggacgtct cgatcgcctg tgtcgaatcg gtgtcgacgg
    20521 ggctgaccgc cggggccatc accgtctcgg cggctgacgt ggtcgactct ttcgtaaccg
    20581 acacgcgcca aggcggctcg ggcgtcggct cagtccgagg cgaatccgca tcgacctcgc
    20641 tgaccgtcgt cagcgctgcg ccgcgggcca atgcgagctg ggtgtcgacc gaatcggaga
    20701 cgggtatcgg cagagcctcg aggatcggcc gggcgacatc gtcgagatca gtgtgtgcgc
    20761 cgacaaggta aaggctctcg ggcaaccaac cgtctttacg gaaaaccgtc ctcaacgact
    20821 caaccaggtc ctcggtggtc tcccgcgtgt cggtgatcgc ggtgcgcacc gtcccggcct
    20881 cggtcggaac gaccatgatc gtcgctgcat cgggctcgag aatgcacagg ccagtcgatg
    20941 tgtgttcgct ttgacgcccg agctcggtcc cccaagcctg ggcagcattg cgcagcgaaa
    21001 ctgggtgaac gttgtggaag cccaagtcgg acaatgactt catcagcgcc gcggcgttcg
    21061 cctcgacctc gtcggtccac gtcacgtgga ccgtgcccac ctggtgaccg cttgccgtcg
    21121 cgatcgcctg cgcgccgcgc acagccgccg cgtgctggga cgtgtcccca gatgcgccgt
    21181 cgtcgccggt ctggacatcg aatgtgtcgt ggtcgagggt ggcggcgtcg gggccttgtc
    21241 cgtcgagcag cacccacccg aaactggtgg acgtcatcga cagtcccaac actgtctgca
    21301 ccttttggcc ccactctcgt cgtcgaatcg acgtcgccgc tgcgggtcaa gcgcccaccc
    21361 gaagcgagca tagtttgctc gccctcgcac gtcatctcgt acggagaaga cgcgcaacac
    21421 cagcgctgtg gcgtttatcg atgggccgac cagggacgtt accacgcgac gcgcggcgcg
    21481 aaattgacgg ccaccaacgg aaactcgcgc ggtacggcta gcccggcaag cgttgctcgt
    21541 ttcactcgga cgtgtttacc gcctgcgtat cgctttcatt tcgttgcgtg cgggacaacc
    21601 tggcaatcac gcggacagtt gggccacgat ggagcgatca gccgaccaat aggcgtccaa
    21661 agcaaatgaa cgcggctgaa cgcaactccg ccgttggacg attccatatc acctatacgg
    21721 gataatggaa ttacccgcag acagcgttga cgctcttccc ggcagcccct gaccaaaatt
    21781 tgcgcgcgag tcagtttcag accgaaggac tggttgcagg gctttgaccg aaatgttttg
    21841 ctaccaagat cagtccgcaa gatttgcggc tgatttggaa tagctattga cccaaactgt
    21901 gcgacaatgc gcatcgaaat acctaccgac gagatcgctt gctggtggga ggagaccaaa
    21961 atttcgacgc atctgcgcct cgcagcgggc atctgcctgc ttaccacggg actcctcatt
    22021 ggcagcgccg gcggagcaat cgccacagcc gacaccgaat cgaccggttc gaccagccag
    22081 agccagggtg ccgacccgtc gactcaaagc gtcaacacag ccagcgcgcc gaccgcgagc
    22141 agtaccgaac cggcaactac taagaccccg cagaatgcgg tccgcaccgc tctactaggt
    22201 gttctccaga agattcgcac gctcgggaaa gtccagcagc agccggtgaa gaaaccggta
    22261 gcagaccctg ccactgccga tagcaagccc gacggcacgg aaggcgatgt cggcgctgcc
    22321 gtcaccgaac ccgtcgcaca gtcggatgtg gtcgcctccg attcgaacgc gaccgagccc
    22381 gagacttacg gcgccgcgtc ggaatcaact gaccccgcgc cagccatcga cccggtgccg
    22441 ccggtgccgc tagggccacc cccagtcgtt ccggcgtcaa acgtgctcga gccggtgaca
    22501 aatgcgatcg cgagagtcgc tagtgcagca ttggcggtgc ctttcgtgct cgcatcgctt
    22561 cccacttccc cgacgccggt cgccgatgtc atcgcggccg tgcaaaacat cctcatcgcg
    22621 gttaacgatg cgatcgcacc catcgtgcaa ctgccgtccg atttcgtctc gctgctcgtc
    22681 gcagccggaa tgagcgccag cccggtcgac tcgatcggta acagtgctgg cgctcgtttg
    22741 tccgccgcaa ccggcacggc gccggtgctc ccctttatgc ccatggttcc gccggtaccg
    22801 ccgaattccc ccgtcggggt catgccgctg cttggcgacg tcaccgcacc cctcacgctc
    22861 ggcgggctcg ccgcagccgg gttgagcgca gatttgtcgc tttccggtac ggcaccgctg
    22921 acagccgacg gcgccggtcc gacgggcgcg ctgtcgttac tcgagcacac cgtcagggcc
    22981 ttcctcgccc ccgcgtcgct gtcggcgctc gccgctctcg cgttgccggg cgtcgccggg
    23041 ctgctgatcg tttgcgcggc ggggatgcgg gtcggatacc gccaagccaa ggcggcgttg
    23101 gcagtccgaa cgaccgggat ttcgcggttc gcacgccagg ggcccctggg tgtcgtccgt
    23161 tcgggctcct tggtcacctt gcatccgcgc tcatctggcc cccagcattc gcgcccgcga
    23221 cgtgttgtcc gtcctgacgt gtcccgcgcg gcgcctcttc tcgaacaggt cgcctagcac
    23281 ccagccatac cggccatgac gcgggagatc tcacgtcagg cattcgtccg cggcgcgctc
    23341 ggcggtgtcg tcgccggtgc actgctcggg tcgtgccgct caacagcgcc agagccgccg
    23401 gcctcgccct cggccgcaag cccgtcgccc ccggcctcgg tggccggccc acaggactgg
    23461 agtgctctcg acgaagcgat cgacggtctg gtcgtcctgc catccagcgc cgactacgcc
    23521 gcggcgaaga gcgtcttcaa cgctcgattc gacgactcca caccggccgc ggtcgtcgtc
    23581 gtgaaatccg tcgacgacgt gcagagggca gcggagttcg cggccagaaa caacgtcaag
    23641 atcgccgcgc gtagcggcgg gcattcctac atcggcgcct caacggcctc cggtgcaatg
    23701 atcatcgatt tgcgccaact ggctggcgaa atcacctacg acgatggtcg cgggcttgcg
    23761 acgatttccg ctgctgtaca actggattcg gtgcagacgg cgctcgcggc gcacggacga
    23821 tcgcttccca gcggcagctg cccgaccgtc ggcgtcgccg gtttgacgtt gggtggtggg
    23881 ctcggctccg atgccaggcg ttcgggcctg acgtgcgacg cgctggtgtc ggcgtccgtg
    23941 gtattgccca gcggcgaagc gatcaccgct tcggcggacg accatgccga tctgttctgg
    24001 gcgctgcgcg gtggcggggg tggcaatttc ggtgttgtca cgtcgttcac gttccgaacg
    24061 tttcctatcg ccgaccggga cgtggtcact ctcgtgtttc ccgagggttc gacggagcag
    24121 gcgatcctcg gctggcacga ctggctgcac gatgccgacc gcgcgatctg gggcatggtc
    24181 aacatcaccg tgggtacgga cgctggcggc tgcacagtcg tcttggcgac gccggctggc
    24241 gacgggcgta gcggagcgag tgacctcagc agcgtgattg gtgtgcggcc tgtcagcaac
    24301 acgagccgaa ccctcagtcg catggacttc gtccactact tcgaaggcgg cgctcaggca
    24361 gcacggccgc gtgcattcgt cgcgggttcc gacatcatcg gggcgatgac gtctgccgcc
    24421 gcggagtcga tcgtcgccgc gacgtcggcg tggccacccg acgccggttc cgcgacggcg
    24481 gtgatcgaat cgctgtccgg cgccgtacgc gatgtcgcgg ccgacgacac cgcgtttcca
    24541 tggcgacgtc aggcggcatg cattcagtgg tataccgagc cgccatcgca ggcggcgatc
    24601 gaaaccgcgg acaaatggct ggccggtgcg catgcggcgg tgcacgcgca ctctgtcggc
    24661 ggctacgtca actacgtcga accggacacc ccggcagcgc gctatttcgc cggcaacctg
    24721 gaccgactta cggccatcgg gcgaaagtac gacccgggcg gtttgatgaa ttccggtatg
    24781 tgacatccgt cggtcgcggt gcgacatacc aatatgaaca ctccaggctc cttcagaatc
    24841 acacgcagtg tcggggctgc ggcagcggcg gcatgggcgc tgccgattgc ttttatcgaa
    24901 ccgccgtccg cggccgccga tccgtgccca gacgtcgagg tcgtgttcgc gcgaggcacc
    24961 accgagccac ctggggtcgg tggcgtcgga caggcgtttg tcgattcgct tcgatcgcaa
    25021 cttggcgcac gctcggttgg ggtgtacgcg gtcgattacc cagccacccg ggacttcgtc
    25081 cgcagcgcgc ctgccggtgc acgtgatgcg agcacccata tccaggccat ggcttcgaac
    25141 tgcccgaaca tccgcatggt gctcggcgga tactcccaag gcgcgggggt gatcgacatg
    25201 tccacgacgg cgatgccacc gcaggtcgcc gatcatgtcg ctgccgcagc attgttcggg
    25261 gctccgagga gcgcctttgc cgactccctc tcccctggcc cgctgcccgc gatcggcccg
    25321 ctctatgcag ccaagaccat cgacctgtgc gtgcccaatg atccgatctg ctccaacggc
    25381 tgggacatgc gggcgcatgg cgcatacgtc gagaccggaa tggtcaatca ggccgcgact
    25441 ttcgctgcga gccgactcta ggacgggcga aaaatgccga tgctcaacac aatcctggac
    25501 gccttcgccc agttccgacc agcaagttct tagagacttg ccagaaaagg gtaacggttc
    25561 gtctgcaggc tacggcgaat tcacctgcaa gccacttcgt tccgaacgac ctcgtaacac
    25621 tctgcgactg tcgcgcgaca tagttgacac cgctgggagt cgggaggtcg ggcatggcga
    25681 agccgcagta cgtcgctacc gctgtcgtgc tggcagcatg gggcaaactc aagggcgcga
    25741 gctcccttga cgagggcacc cgaatcgaag ctgagtgttt tcccccccga cgcgcacggg
    25801 gcgtcgcggt gtgtcagcat aggtccgtga gtccacgcca catcgctgtc tcgatcggcg
    25861 ctgcggcagt ggcgacttcg gttttgcagg ctgcgccgat accgctagcc caggcggcgc
    25921 cctgccccga cgccgaagtg gtattcgccc gtggcaccac cgaggcgccc ggtcccggtc
    25981 ccacgggaga cgcgtttatc gactcgctgc gctcgcgcgt cgcgggcaag tcggtcgggg
    26041 tgtacgcggt cgactaccct gccaccaccg acttcccaac cgctgtcgag ggcatctcgg
    26101 acgcgcgcac acacatcctt gcgaccgcgg ccagttgtcc taagaccaag atggtgctgg
    26161 gcggattctc tcaaggcgcg gcggtcatgg gcttcgtcac cgcgagcgtt gtgcctgacg
    26221 gggtttcagc tacagaagtg cctgcgccga tgccgcccga ggtcgccaac cacattgcgg
    26281 ctgttgccct cttcggaaaa ccgtcgacac gcttcatgcg tgcgatcaat gacccggacg
    26341 tcacaatcgg cccgcagtac gtgggcaaga ccatcgactt gtgtgtagac aacgacctgg
    26401 tttgcgatat gaccggcaga agtttctccg cgcacaacca gtacgtcgag gccgggatag
    26461 tcgaccaggg cgcgactttc gtcgcgaatc aactccaggc gagttgggcc gcggacgcgg
    26521 cggtggcacc tactgagggt gcgactccgt caccgcagac accgccagcg ccgctgccac
    26581 tttcggctgc cgcaggcgcg gctgctcccc ccgccggaat tgcaggcccc acaccgcatc
    26641 tgcccacggg gcctgttcct cccgggccgc ccgcgactcc agtggcagcc cctccgcccg
    26701 ctgggcctgt catctgacgt aaacaacccg acgctcgccg atgtcgggac gagcgcgata
    26761 cggacagatc cggtgcctcg ctaccgatcc caacaaactg cggcgaattc ggctgcgctg
    26821 cagcaatacc gcggtggcgg acggcgacct tgcggtgtgc tgtgtagcga ctgggtattc
    26881 gccgtgtaaa ccctgcgtga cgttccacca aaggcgtgcg cgcaatttct gccgtctttg
    26941 cttgaatccc aatgactttg acctgcctcc acgtcactgc ggatcgcaaa tttggcatgc
    27001 gccgacactg ccggcgttgc tgaccacact cgactgttcc gggcggccgt acgtcggcac
    27061 ctcgcgaaac acgttggcgc gcgccgatga aaagcttgcc tgatggttcg ctggcctaca
    27121 taatcacccg gtgacccaac tgaagagtcg tatttgtgcg ttcgtcgtcg ccttgatctc
    27181 cgtgttttcg atgctgtccg ttccggtagc tgcagccgag gccgccaaca tcaccgacat
    27241 ccagcacgtc aacgaccgct gggacaaggt ctcggtctac tccccatcga tgaacaccgt
    27301 gatcgtcaac gacgtcttca aggcacccaa gtcaggtgcg cccgtcttct acctgctgcc
    27361 gggcatcgac ggtggcgacg acatcgccgg cggcggtatc gcttccggcg gcacgagctg
    27421 gttcggcatg accgacatcc agggcttctt cggcaacaag aacgtcaacg tcgtctcccc
    27481 gctcggcggc gcgttcagct ggttcacgaa ctgggtcgcc gatcccggca agcagtacca
    27541 gacctacatg acccaggaac tgccgccact gatcaacaag cagtacaaca ccaacggccg
    27601 caacgccgtc ggcggcttat ccagcaccgg tggcaccgcg atcgactacg ccgttcaggc
    27661 gccgggactg taccgggccg tcggctccta cagcggtctg ctcaaccccg cggatcagcc
    27721 ccagcaggtt tcgatgacgc tgatgggcgg cggacagagc gccgacgcca tgtggggccc
    27781 agcgggcggc ccgctttggg tggcccacga tccatccaag aacgtcgcga agctcaaggg
    27841 tgtcgctgtc tacgccgccg cgtccgccag cggcggggta ggcaccgtcg accggctgcc
    27901 cgaagacttc ggaccgaact tcaccggtgg gctgatcgaa cgcatcgtcg ccgacagcac
    27961 gaaggtgttc gccgattccg cggcggccgc cggcgtaccg atcaactacg tcgttcggcc
    28021 cgacggctcc cacacctggg gcctgttcga atcggagatg caagagtcct ggaacaccac
    28081 cgtcggaccg gcgctcgggg cctagggcct agccatcaac ggcttggtgc gttgagttct
    28141 tagccggaac agacgcgctc agccgcgcgg cgcccgccgc gatcacgaag catccgagct
    28201 cggtccaccc tgcgaaatcg ctgaggccgt gcttggccag cagcaggagc gcgacgcccg
    28261 aaacgcaaat cagcgcaccg gcggcagtgg catgggcgcc agtggctccc actggcgcat
    28321 cccaccacgg cagccgccga tgctcgaggg gtgacagtag tcggaaagcc acggccatga
    28381 cggccgcgaa gacaaccgcc cgcagcgcaa gaagggccca aaaccctggc gcatcaacgt
    28441 catacgcgtc gagaccgacc gcgttcagcg cgaaggtggc caccgcgatc gccgggacgt
    28501 gccagaggta gagcgtcatg gctcccccgt tgcccaccac gaccaggcgc cagatgcgcg
    28561 gccgggcggc ccaccggctg actgcgcccg cggtagccac gaacgcgcac gacatccagg
    28621 tgcagtgcag cgcgagcaac agcgttggcg gcgacacgtt cgacattcgc tccatgccgg
    28681 tcacgacgag ggatatttcg tagggtccaa ccatcgcgag caccacctgc gcgacgaatg
    28741 cggcagcggc agcggccagt gcggcgcgtg ggccgatcag tcgacgcgcg tacgcgacgc
    28801 cgatcacgac ggggatcaac cacacgatca cgaagttggc gctgccagcc gtggacgtgc
    28861 cgacggcgat ccggatcccg tcgatcgcca ccgcagcggt aatcagcgac gcgacgagaa
    28921 cagcaactcc aacgcccgtc cgcagacgcg tgagggccgg gacgaatgcc agcaccacga
    28981 ggtacacgcc gaggaaccac agcagcgcca cgcactcgcg cccgagtccc gccgcggatt
    29041 cggcgccaag ggtcatccgc gccacgagga gaccgaccga ccatgccgcg aggtaccaga
    29101 agacgggccg acacaggcgt tgcgacctcg tgaaaagcca tgtgccccac ggggttccgg
    29161 tgtgccaacc gtatgcgccg gcggcgccgc ccgcgaggaa gaacagcggc atgacctgga
    29221 cgatccaggt gatcggggcg atcgcgggta gctcaccgag gaggttgccg atgcgcagtc
    29281 caccggagtc gatcgtggcc agcagcaacg cgcaatgccc gaacatgacg acgacgagcg
    29341 cggcaagtcg ggcgacgtcg accgcgcggt cgcggctggc cggggtgttg tcggcggccg
    29401 tgcgggcgtc cggcgcaggc gtggtgagcg gcatgatccc agcatgccgg gtgggggcac
    29461 catgcggatg agtagtacta cgcgctaatc tctcgtccgc ggcgtagtgc cgcagtttgc
    29521 ccggagggaa tttcgacatg cgcatcctga atgtggccgt gctcgtcgcg gcggtcgccg
    29581 tcttcggctc gtccggtgtg gctgcggccg caccgaagga ctactgcgcc gatctcaagg
    29641 gcgggattgc gggcgcgacg tgcgtcattc agttgtccga cgccggctac aacgtcgata
    29701 tcagctttcc gaccgactac cccgatcaga agtcggtggc cgactacatc actcagacgc
    29761 gcgacgggtt cctcaacgtg gccaaatcgg gagcgccgcg cgacaccccg tacgagctga
    29821 acatcacctc gaccaactat gggtcagcga taccgccgcg tggcacgcag gccctggtgc
    29881 tcaaggtcta tcaaaacgtc ggcgccgcgc atccgcagac gatgtaccag gcgttcaact
    29941 gggatcagtc gtatcgcaag ccgatcgtct gggacacagc gggagacgac aagaaaaccc
    30001 cgctgtggcg ggtcgacgat ccgctgaaga ccgtggcccc gatcgtgcag gccgagttgc
    30061 agaagcaaat ggcaccgccg gtgccggccg tgccgcccgc gcagccggga cagccaacag
    30121 cgaccccgcc acctgcgccg acggcaccgc cggtggcgat tgcgccgact gccttgtatg
    30181 acccgatgaa ttaccagaat ttcgctgtga ccaatgacgg cgtcatcttc ttcttcagcc
    30241 aggggcagct gctgccggag gcggccgggg ccacccaggt gttggtccct cgcgccgcga
    30301 tcgacccgat gctggcctaa cgctcaaccg ttactgaaga ttgccacgcc gcgtcggaca
    30361 cgacagtaag gtgcagctcg tgggcaggaa cctgttagcc aacggccggg gccgctggct
    30421 ggcgctcgcg gcctccgtcg tggttgctgc cggcatgttc tatgcccaag acacaaagcg
    30481 tccgtgctgc gccgagaacg cggcggtagc gccgacgtcc caggcgcctg cacctctgcc
    30541 agccgccgcg ccgtctgtcg ccagtccgac ggaggcggaa ctgttggctg cgagcgcccc
    30601 tgtcactgct ggccaggatt tccagttcgc gttacccgct ggcatcgcgc ctgaagaggg
    30661 attgcagatc cacacaatct gggtggcccg tgccatcagc gtgctgtttc ctgagatcaa
    30721 aaccatcggt gggtaccggc aggatgcgtt gaagtggcat cccaacgggt tggcgattga
    30781 cgtgatgatc ccgaactatc acagcgacga gggaatcgag ctcggcaacc agatcgccgg
    30841 gttcgcattg gcgaacgcga aacggtgggg cgtgatccac gtgatctgga ggcagggctt
    30901 ctacccgggc atcggcgcgc cgagttggac ggcggattac gggaatgaaa ctgctaacca
    30961 cttcgaccat gttcatatcg ccaccgacgg cggcggatac ccgaccggca aggaaacgta
    31021 ctacatcggg tctatgaagc tctgaacggt cttgggccgc caccctctcg aatggcgggc
    31081 caactcaccc gaattcggtt gcggcacatc aagtgtccgc agcagtagcc gttgcacgga
    31141 agtggccgat ttcgcgcgac tttcatgttt ggtcgcaaag gggccgaccc ggcttggcgg
    31201 ccctgatcag ggttttcgtg cctctggcag ccggggcccc acgtcgattc cgttgagggc
    31261 gcaacgacga gttcccttgc atccccatat gaggtatgtg cacgtcagtt gcagactatg
    31321 ctccgcccac gggaggaagg gcttacgcct gcaacacatt aggggggaac caagatgact
    31381 gtcgtctcgt tcattgctat tcacccaaag ccaggtggca attgggaaga gctccaaaaa
    31441 cagctgaaga aaggaagtga cctcgcccgg aagcatggcg ctgagaacgt gaccgcgttg
    31501 tggaacgtgg ccggcggaac cgccaccgga acggtgatct tgctctctag ctgtgccgat
    31561 tggacgagtt acgggaagat tcaggacgcc atgttggctg atcccgagat gcaggctctc
    31621 atggccgatc cgaacagtcc gatcgccagg tgggacacgt atgtcagcca gacgatccct
    31681 gacatctaga agctgcgccc cacgataggt tcggcccgcc accccttcga gtggcgggcc
    31741 gaacgattgc tttgggtgat cagggttgtt tggtcagaac cggcagggtg ccgcccttga
    31801 ggaccggcgg ccgcaccaca ttgcgcttgg gcgcagtggt gacgacgatc tttccgtcgc
    31861 ggcagctgac gatcttgcca tcaacgaaga catcctggcc gttgtcgatc gggatgtcat
    31921 aaccgtcagc gtcggtgtag gtacagccgc caccgccggg ggccgggtcg ttgtcgacgg
    31981 cgtaggcggt cgccggggcg atggcgagca cggcgaaggc gccgaggacc gtcgccgcgt
    32041 acttgaagcc cgtgaccttg cgggtggttg aagtggtcat tgtggattcc tctcgtggat
    32101 ggctttggga ctgcggtggt ttggttgttg agaggaatac tgctggttaa gcggtggctt
    32161 cgtaatcgcc cagaagccag agtccactgc tgcgatggga agagtttcct atccgccagg
    32221 taaggacggc gcagaaggct tacatccagc tcggcggtct gatcgaattc gccaggtcga
    32281 ccagggtgta gcgatggcgc cggcgcggcg agtgccgcgc cagttctcga agggcctcct
    32341 cgagacccgt gcgcaatccc tcctggtcga acacctggct gaggatcggg tcggtggcgg
    32401 caggcgtcgc gccggatcga agccagtcga gggcggtacc gagcacaagc gcgcgcacct
    32461 gcagggcgcg cggctcagtc tcggacaact gaccgacgcg cctcgccgaa tcgcgcaggt
    32521 cggcctcggt gatttcggcg atgggccgcc cgtcgagcag catcaacacc gacgtcagct
    32581 gggcttcacc gtagtggcgc gaggttaacg ggacctcgtc gagcacatcg atcgcgacgg
    32641 gccgatcgcc gcgcgcggcg agttggcgag ccaagccgaa cgccgcactc accatggaat
    32701 ggtcggtgcg ccacaacgtc cggtagtacc gctcgcacga ctgccgcaat cgttcggcat
    32761 tccgtgcgtc ttcagtaccg agcatcagct cggccgtggc ggccgacgcc accttcggcg
    32821 cggcttcgcc gggtaccgcg gcgaccaccc gctggaaaca cttcagtgct gcccgtaggt
    32881 ttccggaatc cagcgcggag atcccgtcgt cccagtccat ccgccaatcg ctttcccggt
    32941 cctcggtcgg ggccggcgtc ggcagtgcac gggcgacatc gcgtgcggtc agccggacgg
    33001 ggtgcaggcg cccgtcgacg aacacgtcgg tgcgctgcgg cgccaggtcc gctccgaact
    33061 ccgagccgtg catggtgaag agcaccgacg tccctgggcg cggcacaccg gtctgctccg
    33121 cgagaacttc acgcagcact cccttgcact gggtcgccat ctcgtctgct gacgagaacc
    33181 gctgcgccgg acggggattc gtcgcgcgca ccagcagccg gtaaaacgat tcataccgtt
    33241 ggaacagggg cacgtcgtcg ggttcgggca acgtctcggc gtaggggtca tcgggcatgt
    33301 cgacggtcag tttggccagc gttcggccga cggtgtagat atcggtcgcg accgtcggac
    33361 cggttcgtac gatctcgggt gcctgaaatc ccttggtgcc gtagatgtat ccgaagtctc
    33421 cgatacccga caccgcgccc atgtcgatca gctcgacgtt gtcctcggtg agcatgatgt
    33481 tctccggctt gagatcgttg taaaccaggc cgagcgagtg caggtaggac agcgcaggca
    33541 tgacctcgag cacataaccg agcgcctgct ccaccggcat catcggattg ggttcacctt
    33601 gcggcgcttc cgctcgctgc ttggccagga ttgcctgaag cgtggtaccg ccgaggtact
    33661 ccatcacgat gtagccgatc ggacgtccat cgaagcccgg gtgttcgacg aagttgtaga
    33721 tcttgacgat gccgggatgg ttcaccatcg cgaggaactg acgttcggcg atggcgatcg
    33781 cctgcgcctg c
//
LOCUS       DASRJA010000686         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_855069,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000686 DASRJA010000000
VERSION     DASRJA010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855069"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="VFC01_30875"
     CDS             complement(<1..163)
                     /locus_tag="VFC01_30875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HZN84056.1"
                     /translation="MRAIAAVLAIAVTTLASCSSTEPAPAPDLSRDLAGKVTADGMYT
                     HLRKLQDIAD"
     gene            complement(160..1668)
                     /locus_tag="VFC01_30880"
     CDS             complement(160..1668)
                     /locus_tag="VFC01_30880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HZN84057.1"
                     /translation="MVRKTLAVLTVAIAVAALGGCGRELRPQPAQPTATQTTAATPAT
                     DFAATLQKRMTADAMIAHLKKLQEIANAHDGNRALGTSGYDASVDYVVNMLRDKGFDV
                     QTPEFEVHLPYADEPLLTVNGDKVAAKPLEFTKGTPPDGVTGPLVPARVEDTPGCTAS
                     DYDGLPVAGAVVLVDRGKCPFGAKQTVAAERGAVALIVANNEDGDGMGGTLGEETDVK
                     IPVISVTKATGERLRAAPGPTTIKLNAGVRNEKTRNVIAQTKTGSTANVVMIGAHLDS
                     VAEGPGINDNGSGVAAVLETALQLGNSPQVTNAVRFGFWGAEEHGLLGSRNYVQTLNE
                     DALKDVALYLNFDMLGSPNPGYFTYDGDQSTPPNDNNGVPRVPEGSAGIERTLVAYLK
                     GADKPAEDTSFDGRSDYDAFTLAGVPAGGLFSGAEDKKTAAQAERWGGQADEPFDPNY
                     HKATDTLDHIDRTALEIMGGGVAYAVGLYAQDQSGRNGVPIRDDRTRHVLES"
     gene            complement(1732..>2373)
                     /locus_tag="VFC01_30885"
     CDS             complement(1732..>2373)
                     /locus_tag="VFC01_30885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN84058.1"
                     /translation="QASTAYGAAPLPPEKAAMGVAIFGVPVLMVLAAMTVTGEYRSGM
                     IRTTFMATPNRTLVLAAKAIVAALFSAIYAAAMTIAAVVVARLMAPPLVGSNLSFVDA
                     WRLVGAIALYAALTAVLGVGVAALLRHSAGAVAVLLLWPLLVEPVLGNLPSISSEVGP
                     YLPFANLFRFIDVQWLYPVYDMPWGEVGSLVYFAIVVAVIYAAAIVVVNRRDA"
BASE COUNT          372 a          800 c          784 g          417 t
ORIGIN      
        1 cgtcggcgat gtcctgcagc ttgcgcaggt gcgtgtacat gccgtccgcg gtgacctttc
       61 cggcaaggtc gcgggaaaga tcgggcgcgg gtgcgggttc ggtggacgag cacgacgcga
      121 gcgtcgtcac cgctatggcc aggaccgcgg cgatggccct catgattcga ggacgtgccg
      181 tgtgcgatcg tcgcgaatcg gcacgccatt gcggccgctc tggtcctggg cgtacaagcc
      241 cacggcgtat gccactccgc ctcccatgat ctcgagcgcc gtgcggtcga tgtgatccag
      301 cgtgtcagtt gccttgtgat agttcggatc gaatggctcg tcggcctgcc cgccccagcg
      361 ctcggcctgc gcggccgtct tcttgtcctc ggccccggaa aagaggccgc ccgcaggcac
      421 tccggccagg gtgaaggcgt catagtccga ccgaccgtcg aaggaggtgt cttcggccgg
      481 cttatcggcg cccttcagat aggcgaccag cgtgcgttcg atgcccgcgg agccttcggg
      541 cacccgcggc acgccgttgt tgtcgttcgg cggggtggac tggtcgccgt cgtaggtgaa
      601 gtagccgggg ttgggtgagc cgagcatgtc gaagttgagg tagagcgcaa cgtctttgag
      661 cgcatcctcg ttgagtgtct gcacgtagtt gcgggagccg agcagtccgt gctcctccgc
      721 gccccagaaa ccgaaccgca ccgcattcgt cacctgcggc gaattgccaa gctgcagcgc
      781 ggtttccagt acggccgcca ctcccgagcc gttgtcgttg atgccgggcc cctcggcaac
      841 gctgtcaagg tgcgcgccga tcatcacgac gttggcggta gatcccgtct tggtctgggc
      901 gatcacgttg cgtgtctttt cgttccgcac gccggcgttg agcttgatgg tggttgggcc
      961 cggcgcggca cgcagccgct caccggtggc cttggtgacg ctgatgaccg ggatcttcac
     1021 atcggtctcc tcgcccagcg tgccgcccat tccgtcgccg tcctcgttgt tggcgacgat
     1081 cagcgccacg gcgccgcgtt cggcggcgac ggtctgcttc gccccgaacg ggcacttgcc
     1141 gcggtccacc agcaccaccg caccggcgac cggcaggccg tcgtagtcgg atgcggtgca
     1201 acccggagtg tcctcgacac gggcgggcac cagcggcccg gttacaccgt cgggaggggt
     1261 gcccttggtg aactccagtg gcttcgcggc gaccttgtcg ccgttgactg tgagcaatgg
     1321 ctcgtcggcg tacggcagat ggacctcgaa ctccggggtc tgcacgtcga aacccttgtc
     1381 ccgcaacatg ttcaccacgt agtcgacgct ggcgtcgtag ccggaggtgc cgagtgcgcg
     1441 attgccgtcg tgggcgttgg cgatctcttg cagcttcttt aggtgcgcta tcatcgcgtc
     1501 ggcggtcatc cgcttctgca gcgtcgcggc aaagtccgtc gctggcgttg ctgcggtggt
     1561 ctgggtagcg gtcggctgcg caggttgggg gcgcagctcg cgaccgcaac cgccgagcgc
     1621 cgcgacggcg atcgccaccg tcagcaccgc cagggttttg cgcaccatcg ccaccaccgt
     1681 agtccgacta accaggttgg cggaccgagg ccatgacatt tcgatccccg gtcaggcgtc
     1741 gcgccggttc accacgacga tcgcggcggc gtagatcacc gcgacgacga tcgcgaagta
     1801 caccaacgag ccaacctcac cccacggcat gtcatagacc gggtacagcc actgcacgtc
     1861 gatgaaccga aacaggttcg cgaacggcag atacggcccg acctccgaac tgatgctggg
     1921 caggttgccc agcacgggct cgacgagcag cggccacagc agcagcaccg cgaccgcacc
     1981 cgccgaatgt cgcagcagcg ccgcgacccc gactccgagc accgcggtca gcgccgcgta
     2041 cagcgcgatc gcgccgacca gtcgccaggc gtccacgaag gacaggttcg acccgacaag
     2101 cggaggggcc atcaggcgcg cgaccaccac tgcggcgata gtcatcgctg ccgcgtatat
     2161 agcggaaaac agcgctgcta caatggcttt cgctgccaac accagggtgc gattcggcgt
     2221 ggccatgaac gtcgtgcgga tcatcccgct gcggtattca ccggtcaccg tcatcgcggc
     2281 aagcaccatc agcaccggta cgccgaatat cgctaccccc atcgcggcct tctccggtgg
     2341 cagcggcgcc gcaccgtatg ccgtggacgc ctg
//
LOCUS       DASRJA010000687         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1504739,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000687 DASRJA010000000
VERSION     DASRJA010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504739"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VFC01_30890"
     CDS             <1..521
                     /locus_tag="VFC01_30890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HZN84059.1"
                     /translation="SGIVSYAEIVSSISAKSAGPGTRANIDEFTETTSTALQVVGGAQ
                     RGKAVMIINPADPPVLMRNTVYCLVDGDADHHAIENDVLAMVEKVNAYVPGYRLKQRV
                     QFETFSSERPLYIPETGKFSGTRVTVLLEITGAAHYLPAYAGNLDIMTSAAKATAERI
                     AAHSTETAGAAS"
     gene            518..1537
                     /gene="dmpG"
                     /locus_tag="VFC01_30895"
     CDS             518..1537
                     /gene="dmpG"
                     /locus_tag="VFC01_30895"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HZN84060.1"
                     /translation="MTHDLYISDVTLRDGMHAVRHQYSVEQAVTIATALDAAGVDSIE
                     VAHGDGLAGSSCIYGFGAHTDLEWIEAVAGAVQRAKVATLLLPGIGSVHNLKDAHRAG
                     ATVVRVATHCTEADISAQHIAAARELGMDTVGFLMMSHMTPPAALAEQAKLMEGYGAT
                     CVYVVDSGGAMTMRDVAERVDALRQRLSPTTEIGIHAHHNLSLGVANSIAAVEHGANR
                     VDASLTGMGAGAGNAPLEVFVAAATKLGWYHGCDLHALEDAADDIVRPLQDRPVRVDR
                     ETLTLGYAGVYSSFLRHAEAAAERYDIDARTLLEEAGRRRMVGGQEDMLVDIALDLTT
                     DKQSA"
     gene            complement(1534..1983)
                     /locus_tag="VFC01_30900"
     CDS             complement(1534..1983)
                     /locus_tag="VFC01_30900"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN84061.1"
                     /translation="MYAVTRVWFSMSRDGLLPKWFAAVSPKHRVPVRVTWIVGIGSGL
                     IAGFLPIAEAAELTNIGILLAFAVVCTAVIVLRYRSPDIERGFRTPGMPVIPLIGVAS
                     SIWLTTYLTTVTWLRFAVWLVIGLVIYGLYSYRHSHSNTAASGKPTT"
     gene            complement(1943..>2338)
                     /locus_tag="VFC01_30905"
     CDS             complement(1943..>2338)
                     /locus_tag="VFC01_30905"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN84062.1"
                     /translation="IGLNLPEWMLGAPGSDPDQPGRVVDLFALLLCLLIAFILTRGVR
                     SAARVETLLVWLKVGVVVLIVVLGVFYVNTDNYTPFLPFGWGGVFAGASVVFFAVFNA
                     DLGRRHSRDRDGYVDVHVRRDPGLVLDVP"
BASE COUNT          466 a          787 c          743 g          342 t
ORIGIN      
        1 agtcggggat tgtgtcctac gccgagatcg tgtcgtcgat ctcggcaaag tccgccggcc
       61 ccggaacccg cgccaacatc gacgagttca ccgaaacaac ctccaccgca ctgcaagtcg
      121 tcggcggcgc ccagcgtggt aaggcggtga tgatcatcaa ccctgccgat ccaccggttc
      181 tgatgcgcaa caccgtctat tgcctcgtcg acggcgatgc cgaccaccac gcgatcgaga
      241 atgacgtgct ggccatggtc gagaaggtca acgcctatgt gcccggctac cggctcaagc
      301 aacgcgtcca gtttgaaacg ttcagttccg aacgcccgct gtatatcccg gagactggca
      361 agttcagcgg tacgcgggtg acggtcctgc tcgagatcac gggcgccgcg cattatctgc
      421 cggcgtacgc gggcaatctc gacatcatga cgtcggcggc caaggccacg gccgaacgca
      481 tcgccgctca ttccaccgaa accgccggag cagcatcatg actcacgatc tctacataag
      541 cgacgtcacc ctacgcgacg gcatgcatgc cgtgcgccat cagtacagcg tcgaacaggc
      601 ggtcacgatc gctacggctc tcgacgccgc cggagtggac tccatcgagg tcgcccatgg
      661 cgatggcctg gccggatcga gttgcatcta cggcttcggc gcgcataccg atctggaatg
      721 gatcgaagcg gtggcgggag cggtgcagcg ggccaaggtt gccacactgc tcctgcccgg
      781 tataggcagc gtgcacaacc tcaaggatgc gcaccgcgca ggcgccaccg ttgtgcgcgt
      841 cgccacgcac tgcaccgagg ccgacatctc ggcgcagcac atcgccgccg cccgcgagct
      901 cggcatggac accgtgggct tcttgatgat gagccacatg accccaccgg ccgccctggc
      961 cgaacaggcc aagctcatgg aaggctatgg cgccacctgc gtctacgtcg tcgattccgg
     1021 cggtgccatg acgatgcgcg acgtggccga gcgcgtggac gcgctacgcc agaggctctc
     1081 ccctacaacg gaaatcggta tccacgccca ccacaatctg tcgttgggcg tcgccaactc
     1141 gatcgccgca gttgagcatg gcgccaatcg tgtcgatgcc tcgctgaccg gcatgggcgc
     1201 aggcgcgggc aacgcgccgc tggaggtatt cgtcgccgcc gccaccaagc tcggctggta
     1261 tcacggctgc gacctgcacg ccctcgaaga tgccgccgac gacatcgtcc gcccgttgca
     1321 ggaccgaccc gtgcgcgtcg accgcgaaac gctgacgctg ggctacgccg gggtgtactc
     1381 cagcttcctg cggcacgccg aagcggccgc cgagcgctac gacatcgacg cacggacgct
     1441 gcttgaggaa gcaggccggc gccgcatggt cggcgggcaa gaagacatgc tcgtcgacat
     1501 cgcgctcgac ctcacgactg acaagcagtc ggcttaagtg gtcggcttac cgcttgcggc
     1561 cgtgttcgag tgcgaatggc ggtaactgta gagcccgtag atcaccagtc cgatcaccag
     1621 ccacacggcg aaccgcagcc aggtgaccgt cgtgaggtag gtggttagcc agatcgacga
     1681 cgcgaccccg atcagcggga tgaccggcat gccgggagtg cggaagccgc gctcgatatc
     1741 gggcgaccgg tagcgcaaca cgatgaccgc ggtgcacacg accgcaaacg caagcaggat
     1801 cccgatgttg gtcagttcgg cggcctcggc gatcggcagg aagccggcga tcaggcccga
     1861 gccgatgccc acgatccagg tgacgcgaac gggcacccgg tgcttggggc tgacggcggc
     1921 gaaccacttg ggcagcagcc catcacggga catcgagaac cagacccggg tcacggcgta
     1981 catgaacgtc cacataaccg tcgcgatccc gagaatggcg ccgaccgaga tcagcgttga
     2041 agacggcgaa gaacaccacc gatgcgccgg cgaacactcc gccccagccg aacggcagga
     2101 acggcgtgta gttgtcggtg ttcacgtaga acacgccgag cacaacgatc aggacgacga
     2161 ctccgacctt gagccagacg agcagggtct ccacgcgagc ggcgctgcgc acgccgcggg
     2221 tgaggatgaa ggcgatcaac aggcacagca gcaatgcgaa caggtcgacc acgcggcccg
     2281 gctggtcagg gtcgctgccc ggcgccccga gcatccactc gggcaagttc agcccgat
//
LOCUS       DASRJA010000688         8393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3758608,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000688 DASRJA010000000
VERSION     DASRJA010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8393
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3758608"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFC01_30910"
     CDS             <1..280
                     /locus_tag="VFC01_30910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN84063.1"
                     /translation="KRGDWIENGWKRDPQEYLQAGNIWTTCEPEEPILPGVIDVLGDD
                     FIMFASDYPHWDGEWPESTKHLRTRADISEETRAKIGGRNAQRFYSLN"
     gene            complement(370..1527)
                     /locus_tag="VFC01_30915"
     CDS             complement(370..1527)
                     /locus_tag="VFC01_30915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HZN84064.1"
                     /translation="MRSRAAILHDVGGPWSVEDFELDPPRAGEVLVEMAAAGLCHSDD
                     HILKGDMSVSNEVAREFGLIPMFPMIGGHEGAGTVLEVGDGVSDLVVGDHVVMSFVAV
                     CGHCRWCASGMEYLCDEGAATMAPGMPTDQTFRHHTADGKPLGHLSKVGAFAKHTVVS
                     QHSIVRIEPHLPLVPSALLSCAIPTGYGSAVNRANVRGGDTVVVVGAGGIGTAAIQGA
                     RINGAAQIIAVDPVEFKQKAALTFGATHSVATVADATDLVRDLTYGVMADSVVVAPSL
                     IAGEDVNSYLKLIRKGGTCVLTGMTAQTTRSAKIALQDFILWNKNLVGTVFGSCNPRA
                     DIARLARLYQTGQLQLDEMITKRYRLDDINAAYDELLNGKLIRGVIDFAIS"
     gene            complement(1524..2723)
                     /locus_tag="VFC01_30920"
     CDS             complement(1524..2723)
                     /locus_tag="VFC01_30920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014941546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN84065.1"
                     /translation="MTVSTDSHDQPISLDADPYAFFAHKRKTEPVWRGTIMDTAMAPP
                     EMLPAEEWTLFDFNSVFAAFRDDELFGSEQYNNTIGLVFGPTILGMHGKQHHDHRSLI
                     SKAFRQSALERWEPAVIDPICDHLVDEIKDNGAADLITAITLEFPTRVTAALLGLPEQ
                     DLDLFRRLSFDLISIYIDIEAGLKASVELQTYFQQQVDQRRSKMTNDVIGDLVGAEID
                     GEKLTDEAIISFLRLLLPAGLETTFRSSSNLLYLLLTHPEQLEAVARDRDLIPAAIEE
                     GIRYETPLVSVPRTTTRPVEINGVPIGEGAQVHLCMGSANRDETRWPNADRFDVHRAR
                     RAHVSFAGGIHSCLGMHLARLETRAMLNSLFDRVTDLQLVPDEDSKIVGMPFRSPKTL
                     PVTFRPA"
     gene            2814..3170
                     /locus_tag="VFC01_30925"
     CDS             2814..3170
                     /locus_tag="VFC01_30925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZN84066.1"
                     /translation="MQRRPVCAIEDLPPGSMKLVQAGKFGVGVYNIDGSVFAIANYCS
                     HEGAPLCAGYVVGTNEFAPDVPGQLRRVREGRIARCPWHNWEFDITTGVNLADPQKRV
                     RTYEVDVSDGQVFLTA"
     gene            3167..4219
                     /locus_tag="VFC01_30930"
     CDS             3167..4219
                     /locus_tag="VFC01_30930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN84067.1"
                     /translation="MTVIDTNVQPHFRYNAEIRKYLAPPHKLRSIPDVEQQWYQAPGG
                     DYRQDLYGDGYPGSDPDTVARHLFTEGGVDYAILNPLTRGNIADYLLNSRICAAVNDW
                     LLDRWLEPDSTGKFLGTIRVNPEDVKGAVAEIERLADHPKMVQVGIPLQSREPYGKPM
                     FEPIWEAAAAHNLPVAVHINGGNGVDHAPTFAGHAYTYPGYAAFMPLNSFVHLATLII
                     EGTFGRIPGLRFVFADGGYDILTPLIWRLDTFWMSMRDQTPWVDRYPSEYLRDHVWFC
                     SSALDGPTDASQAERWLDFTDKADLLMYGSSYPHWSTSRPDDVTAGLNATQREKVLWR
                     NASDLYGVTATEGSLA"
     gene            4216..5373
                     /locus_tag="VFC01_30935"
     CDS             4216..5373
                     /locus_tag="VFC01_30935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN84068.1"
                     /translation="MTTVEERTNDFVAQDIAVTIVDTDVHPLPVSAEVLKSYAPAEWK
                     DKLWPTGNAVSPVPHFYDTPDSYKTMSLRVDAAPPGGGVAGSDPDFAAKQLLIDAGVS
                     IAILEPMCDAQLPHAEHVLKATHNDWLADVWLGDNNWHGRWRGAVSVTAQDPHAAARE
                     IERWAGHPYMAEVLMTPQTRGIPFGSPHFDPLYEAAARNGLPVATHLMGQTPFELIPI
                     YPVGNPAHWHDFFASWPLLYVSHLMSLVFDGAFDRHPDLRVVFVEGGFTWAMPVMSRM
                     DRIWEQRKGDLPHVRRKPSEYVREHVRFTTQPLEDVNVGMYREYLEMMDLGDCLMFST
                     DYPHWSYDAPDWAIRRFPADQRERIMRGNATALYGLPATVKALPGEFPATA"
     gene            5351..5776
                     /locus_tag="VFC01_30940"
     CDS             5351..5776
                     /locus_tag="VFC01_30940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84069.1"
                     /translation="MNSQPPPDVSDLVRDYDKCWSSLDFVALADLWERDDPQPIYVGD
                     EYAAPLIGNDELDRHWARVAGRLKAASVSSTLHEYDVVDDTVVRALLLSRWSLTGRES
                     DVQRNGTSWITWLLVRRGERLRIFHQMESQVYLTDSEGE"
     gene            5776..6207
                     /locus_tag="VFC01_30945"
     CDS             5776..6207
                     /locus_tag="VFC01_30945"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZN84070.1"
                     /translation="MELNIRPVPIPDPVSQFYWDSAKDGKLAVQGFEGTDILQHPPSP
                     VPEVPGGPGDDVGDPIAVEVSGRGTLFAFTILHQPFHPGFVDAVPMIIGLTELDDAPG
                     VKILTNIVEADPAELRCGLPMEVVFEQREEFALPQFRPRRD"
     gene            complement(6204..6926)
                     /locus_tag="VFC01_30950"
     CDS             complement(6204..6926)
                     /locus_tag="VFC01_30950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN84071.1"
                     /translation="MRTALITGGSGGIGKACARKLVELGYDVILTARREEPLRAAAEE
                     LGARYVVADATDPVKFEVAVQAAGDIDLVVHAAGVLGGTYARKQTFEQWRATMSANLD
                     SCFVVTSAALPRMKAGSRFVFISSSAAHEPMMARTAYSASKAGMNAFALALAQEVDRD
                     GINVHIVTPGPVETDMLQDVPFEMYAIRAADVAEAVAWLDTVDPSVDVPEIRLHAITR
                     GPSARQPVVPLEAERRVPKRTH"
     gene            complement(6937..7746)
                     /locus_tag="VFC01_30955"
     CDS             complement(6937..7746)
                     /locus_tag="VFC01_30955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZN84072.1"
                     /translation="MTTLGIGPEARHRLSARRTAEIVADELRRQIIDGELSDGDLLPR
                     QEVLVEQFNVSLVSLREALRILETEGLVSVRRGNRGGAVVHAPAKASAAYMLGLLLQS
                     DYVPLADLGTALQELDPMCAALAAKRPDRGETLVPKLKELNDAMAEHIEDGAHFTEIG
                     GQFHDEIVRGCGNHTMIAVVGSLETLWSSHLQWWADETAARGEYPALSKRRIALGIHT
                     KIADAIEAGDAERARKLSARHLADTQAYFMAEDPERRIVALSPQALARRQR"
     gene            7844..>8393
                     /locus_tag="VFC01_30960"
     CDS             7844..>8393
                     /locus_tag="VFC01_30960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN84073.1"
                     /translation="MRLSGASPTPLDDLRVVEISDCIAGAYCGKLCVDAGADVVKVES
                     AGGDPLRRFTATGAGVPKGTDAPLFSYLNSGKRSVTAASDDLLDRADIVITTQREFDP
                     QPLLDRSPGCVVVTITDFGLTGPWSDRAATEFTLQAWSGLTGFRGDPSGPPISIGGEL
                     GEYMAGACAAYGALALRRRVRRG"
BASE COUNT         1465 a         2679 c         2755 g         1494 t
ORIGIN      
        1 gaagcgcggc gactggatcg agaacggctg gaaacgcgat ccgcaggaat accttcaggc
       61 cggcaacatt tggacgacat gcgaaccgga ggaaccgatc ctgcccggag taatcgacgt
      121 gctcggtgac gacttcatca tgttcgccag cgactaccca cactgggacg gcgaatggcc
      181 ggagagcaca aagcatctgc gcacccgtgc cgacatcagc gaggaaaccc gcgcgaagat
      241 cggcggacgc aacgcccagc gtttctactc gctcaactga tcgcgaaggt gcgccgaaac
      301 tgtagttaac gtgccgatca ctcgaacttt ctccgcgata actacagtct cgcggagctg
      361 cggactcgct caactgatcg cgaagtcgat gacgccgcgg atcagcttcc cgttcagcag
      421 ttcgtcgtag gcggcattga tgtcatcgag ccggtaccgc ttggtgatca tctcgtccag
      481 ttgcagctga ccggtctgat acaagcgagc cagcctggcg atgtccgctc tcgggttgca
      541 cgacccgaat acggtgccga ccaggttctt gttccacaga atgaagtcct gcagcgcaat
      601 cttcgccgaa cgcgttgtct gcgcggtcat tccggtgagt acgcaggtgc cgccctttcg
      661 tatcagcttg agatacgagt tgacgtcctc gcccgcgatg agcgacgggg cgacgaccac
      721 gctgtcggcc ataacgccgt aggtcaggtc acggaccagg tcggtggcgt cggccacggt
      781 ggcgacgctg tgcgtggcgc cgaacgtcag cgccgccttc tgcttgaact cgacggggtc
      841 gaccgcgatg atctgcgccg cgccgttgat ccgcgcaccc tggatcgccg cggtgccgat
      901 cccgcctgcg ccgaccacca cgaccgtgtc gccgccacgg acattcgccc ggttgacggc
      961 cgacccgtag ccggtcggga tggcgcacga cagcagggca ctgggcacca gcggcaggtg
     1021 cggctcgatc ctcacgatcg agtgttggga caccacagtg tgtttcgcga acgcgccgac
     1081 cttggagagg tggccaagcg gcttgccgtc ggcggtgtga tgacgaaagg tttgatcggt
     1141 cggcatgccg ggcgccatcg tcgcggcgcc ctcatcgcag aggtactcca tgccggatgc
     1201 gcaccagcgg cagtgcccgc acaccgcgac gaacgacatc acgacgtgat cgccaaccac
     1261 gagatccgat acgccgtccc caacctcaag cactgtgccg gcgccttcgt ggccgccgat
     1321 catgggaaac attgggatca ggccgaactc gcgagcgacc tcgttggaga ccgacatatc
     1381 gcccttgagg atgtggtcgt cggagtggca caggccggcg gcggccatct cgacgagcac
     1441 ttcgccggcg cgtggcggat cgagttcgaa gtcttcgacc gaccacggcc cgcccacatc
     1501 gtgcaggatc gcggcgcggc ttctcatgcc gggcggaaag tgacgggaag ggtcttcggc
     1561 gagcggaacg gcatgccgac gatcttgctg tcctcgtcgg gaaccagctg caggtcggtg
     1621 acgcgatcga acaggctgtt cagcatcgcc cgggtctcca gccgtgccag gtgcatgccg
     1681 aggcaactgt gaatgccgcc ggcgaacgag acgtgcgcgc gccgggcgcg gtggacgtca
     1741 aatctgtccg cgttgggcca gcgcgtttcg tcgcggttgg cggagcccat gcacaggtgg
     1801 acctgcgcgc cctctccgat gggcacaccg ttgatctcga cgggtctggt ggtggtgcga
     1861 ggaacgctga cgagcggcgt ctcgtaccga atgccttctt cgatcgcagc cgggatcaaa
     1921 tcacggtcgc gcgcgacggc ctcgagctgc tcgggatgcg tgagcaacag atacagaagg
     1981 ttgctcgacg accggaacgt cgtctcgaga cctgcgggca aaagcagccg caggaacgag
     2041 atgatcgcct cgtcggtgag cttctcgccg tcgatctcag cgccgaccag atcgccgatg
     2101 acgtcgttgg tcatcttgct gcgtcgctgg tcgacctgct gctggaaata ggtctgtagt
     2161 tcgacggatg ccttcaaccc ggcctcgatg tcgatgtaga tcgagatcag gtcgaacgac
     2221 aaccgccgga acaggtctag gtcttgctcc ggcagtccca gcagcgcggc ggtgacgcgt
     2281 gttgggaact cgagggtgat ggcggtgatc aggtcggcgg cgccgttgtc tttgatctcg
     2341 tcgaccaggt ggtcacagat cggatcgatc accgcgggct cccaccgttc gagcgcgctc
     2401 tgccggaagg ccttggagat caggcttcgg tggtcgtggt gctgcttgcc gtgcatgccc
     2461 agaatcgtcg ggccgaagac gagtccgatc gtgttgttgt attgctccga tccgaacagc
     2521 tcgtcgtcgc gaaaggccgc gaaaacgctg ttgaagtcga atagcgtcca ttcctcggcg
     2581 ggcaacatct ccggcggggc catcgcggtg tccatgatcg tgccgcgcca caccggctcg
     2641 gtcttgcgct tgtgcgcgaa gaatgcgtac ggatcggcgt cgaggctgat cggctgatcg
     2701 tgcgaatcgg tgctgaccgt cacctgtgcc tcccggagca gccgcatcta gcggctgtgc
     2761 cccctatctt tactatttta ggtattctag gtcaaggaaa ccgaagggac atgatgcagc
     2821 gtcggccggt gtgtgccatc gaggatctgc cgccggggag catgaagctg gtgcaggcgg
     2881 gcaagttcgg tgtgggcgtc tacaacatcg acggctcggt gtttgcgatc gcgaattact
     2941 gttcgcacga gggtgctccg ttgtgcgcgg ggtacgtcgt cggcaccaac gaattcgcgc
     3001 ctgacgttcc gggacagctg cgccgcgtcc gcgaaggccg gattgcgcgc tgcccctggc
     3061 acaactggga gttcgacatc acgacggggg tgaacctcgc cgatccgcag aagcgggtcc
     3121 gcacgtatga ggtcgacgtt tccgacgggc aggtgtttct gaccgcatga ctgtcatcga
     3181 cacgaacgtc caaccgcatt tccgctacaa cgcggagatc cggaagtacc tggcgccccc
     3241 gcacaagttg cggtcgatcc cggacgtcga gcagcagtgg taccaggcgc ccggcgggga
     3301 ttaccggcag gacctctatg gcgacggcta tccgggttcc gaccccgaca ccgtcgcgag
     3361 gcatctgttc actgagggtg gagtcgatta cgccatcctc aacccgttga cgcgcggcaa
     3421 catcgccgac tacctgctca acagccgcat ctgtgcggcg gtgaacgact ggctgctcga
     3481 tcgctggctc gagccggatt cgacgggcaa gttccttggc acgatccggg tcaatcccga
     3541 ggatgtgaag ggcgcggtcg ccgagatcga gcggctggcc gaccacccga agatggtgca
     3601 ggtcggcatc ccactccagt cgcgggagcc ctacggcaag ccgatgttcg agccgatctg
     3661 ggaggccgcg gccgcccaca acctgccggt cgccgtgcac atcaacggcg gcaacggcgt
     3721 cgatcacgca ccgacgttcg caggccacgc ctacacctat cccggctacg cggcgttcat
     3781 gccgctgaat tcgttcgtac acctggccac actgatcatc gagggaacct tcgggcgaat
     3841 cccgggtttg cggttcgtgt tcgccgacgg tggctacgac atcctgacgc cactgatttg
     3901 gcgactcgac acgttctgga tgtcgatgcg cgaccaaacc ccatgggtgg accgctatcc
     3961 cagcgaatac ctgcgcgacc acgtatggtt ctgctcatcg gcgctcgatg ggccgacgga
     4021 tgcgagccag gccgagcgat ggctggattt caccgacaag gccgacctgc tgatgtacgg
     4081 gtcgagttat ccgcactggt cgacgtcgcg gcccgacgat gtgaccgccg ggctcaacgc
     4141 cacgcagcgg gaaaaggtgt tgtggcgcaa cgcaagtgac ctctacgggg tcacggcaac
     4201 cgaaggaagt ctcgcatgac gactgtggag gaacgcacga acgacttcgt ggcgcaggac
     4261 atcgccgtca cgatcgtcga caccgacgtg catccgttgc cggtgtcggc agaggtgctc
     4321 aagtcctatg cgcccgcgga gtggaaggac aagctgtggc cgaccggtaa cgcggtctcg
     4381 ccggtgccgc acttctatga cacgccggac tcgtacaaga cgatgtcgct tcgagtcgac
     4441 gcggctccac ccggcggagg cgtcgcgggt agcgatcccg atttcgcggc caagcaactg
     4501 ctgatcgacg ccggggtgag catcgcgata ctcgagccga tgtgcgatgc gcagctgccg
     4561 catgccgaac acgttctcaa ggccacccac aacgactggc tggccgatgt gtggctcggt
     4621 gacaacaatt ggcacgggcg ctggcggggc gcggtcagcg tcaccgcgca ggatccgcac
     4681 gctgccgccc gggagatcga gcgctgggcc ggacacccgt acatggccga agtgttgatg
     4741 acgccgcaga cacgcggaat cccgtttggc agcccgcatt tcgatccgct ctacgaagcg
     4801 gcggcgcgca acggtcttcc ggtcgccacg cacctgatgg gccagacacc gttcgaattg
     4861 attccgatct acccggtggg caatccggcg cattggcacg acttcttcgc atcgtggccg
     4921 ttgctgtatg tgtcgcatct gatgagcctg gtgttcgacg gtgccttcga ccggcatccc
     4981 gacctgcgcg tggtgttcgt ggagggcggc ttcacctggg ccatgccggt gatgtcgcgg
     5041 atggaccgga tctgggagca gcgcaagggc gatctgccgc atgtgcgacg caagccatcg
     5101 gagtacgtcc gtgaacatgt gcggttcacc acgcagccgc tcgaggatgt caacgtcgga
     5161 atgtaccggg aatacctgga aatgatggac ctcggcgact gcctgatgtt ctccacggac
     5221 tacccgcact ggagttacga cgcccccgac tgggcgatca ggcggttccc cgccgatcag
     5281 cgggagcgga tcatgcgggg caacgcgaca gccctgtacg gcctgcccgc gacggtcaag
     5341 gctctcccgg gtgaattccc agccaccgcc tgacgtgtcg gacttggtcc gggactacga
     5401 caagtgttgg tcgagcctgg atttcgtcgc gctggccgat ctgtgggagc gtgacgatcc
     5461 tcagccgatc tacgtcggtg acgagtacgc cgcaccgctg atcggcaacg acgagctgga
     5521 ccgacactgg gctcgcgtcg caggccgact gaaggcggcg tcggtgtcgt cgacgctgca
     5581 tgagtacgac gtcgtcgacg acaccgtcgt gcgggccttg ttgctcagcc gctggagcct
     5641 gaccggcagg gagtccgatg tccaacgaaa cggcaccagc tggatcacgt ggctgctggt
     5701 tcgtcgcggt gagcgacttc ggatcttcca ccagatggag tcgcaggtgt acctgaccga
     5761 cagcgaaggg gagtgatgga actcaacatc cgacctgtcc cgattcccga cccggtgagc
     5821 cagttctatt gggacagcgc caaagacggc aagctggcgg ttcaggggtt cgagggcacc
     5881 gacattctgc agcatccacc gagcccggtg ccggaagtac ctggcggccc tggcgatgac
     5941 gttggtgatc cgatcgccgt cgaggtcagc gggcgcggca ccctgttcgc gttcacgatc
     6001 ctgcaccagc cctttcaccc cggttttgtc gacgccgtgc cgatgatcat cggactcacc
     6061 gaacttgacg atgcgccggg cgtgaagatc ttgacgaaca tcgtcgaggc cgaccccgcg
     6121 gaattgcgct gcgggctacc gatggaagtc gttttcgaac agcgagaaga gttcgcactg
     6181 ccgcagtttc ggccccgccg cgactagtga gtccgcttcg gcacccggcg ttccgcctcg
     6241 agcggcacca ccggttgacg cgccgacggc ccgcgcgtga tcgcatgcag ccggatctcg
     6301 ggcacgtcga ccgacgggtc gaccgtgtcc agccacgcga cagcctcggc tacgtcggcg
     6361 gcgcgaatcg catacatctc gaatgggacg tcctggagca tgtcggtctc gaccggcccc
     6421 ggcgtcacga tgtggacgtt gatgccgtcg cggtcgacct cttgcgccaa ggccagggcg
     6481 aacgcgttca tacccgcctt tgaggcggag taggccgtcc ttgccatcat cggctcgtgc
     6541 gccgccgatg acgagatgaa cacgaaccgc gagcccgcct tcattctggg cagggcggcc
     6601 gacgtgacga cgaaacacga atccaggttc gcggacatcg tcgcgcgcca ctgctcgaaa
     6661 gtctgcttgc gcgcgtaggt tccgccgagt acgcccgccg cgtggacgac gaggtcgatg
     6721 tcgccggccg cttgtaccgc gacctcgaac ttcaccggat cggtcgcgtc ggccacgaca
     6781 taccgagcgc ccaactcctc ggcggccgcg cgcagcggtt cctcgcgtcg cgcggtgagg
     6841 atgacgtcgt agccgagctc gaccagcttg cgggcgcagg cctttccgat gccgccgcta
     6901 ccgccagtga tcaaggccgt tcgcacgcaa cacctttcag cgctggcgtc gcgccaacgc
     6961 ctgcggcgag agcgctacga tgcggcgctc gggatcctcg gccatgaaat aggcttgggt
     7021 gtccgcgaga tggcgcgccg agagtttgcg agcgcgctcg gcgtcgcctg cctcgatcgc
     7081 atccgcgatc tttgtgtgaa tgcccagcgc gatgcgacgc ttgctcagag ccgggtactc
     7141 gcctctggcc gcggtctcat cggcccacca ctgcaggtgg ctgctccaca gcgtctccag
     7201 gctgccgacc accgcgatca tcgtgtggtt gccgcagccg cgcacgatct catcgtggaa
     7261 ctggccgccg atctccgtga agtgggctcc gtcctcgatg tgttcggcca tggcgtcgtt
     7321 gagttccttg agtttgggaa ccagcgtctc gccacggtcc ggacgcttgg cggcaagggc
     7381 cgcacacatc ggatcgagct cctgcagcgc ggtaccgaga tcggccagcg gcacgtaatc
     7441 gctttgcagc agcaggccga gcatgtaggc cgcgctagcc tttgcgggcg catggacgac
     7501 cgcgccgccg cggttgcccc ggcgcacgga caccagtccc tcggtctcca ggattcgcaa
     7561 ggcttcccgc aacgacacca ggctgacgtt gaactgctcg acgagcacct cctggcgggg
     7621 cagaaggtcg ccgtcggaca gctcgccgtc gatgatttgc cgccgaagct cgtcggccac
     7681 gatctcggcc gtgcggcggg ccgacagtcg gtggcgggcc tcagggccga ttcccagtgt
     7741 tgtcatgtct tagcaatgtt aacagtgata ggccttctac cagggggcta cggcatcgct
     7801 atccgacaaa agttactaag atgataaaga aaccagggag gatgtgcggt tgagcggcgc
     7861 ctcgcctacc ccgctggacg atctgcgcgt cgtcgagatc agcgactgca tcgccggcgc
     7921 gtactgcggc aagctttgcg tcgacgccgg cgccgacgtc gtgaaggtgg agtcggccgg
     7981 cggagatcca ttgcgccgct tcactgcaac cggtgccggc gtgccgaagg gcacagatgc
     8041 accgctgttc agctatctca actccgggaa gcgcagcgta acagccgctt ccgacgactt
     8101 gctcgaccgc gccgacatcg tgatcaccac gcagcgggag ttcgatccgc agccgctcct
     8161 cgaccggtcg ccggggtgcg tcgtggtgac catcactgat ttcggactga ccggcccatg
     8221 gtcagaccga gccgcgaccg agttcacact gcaggcctgg tcaggtttga ccggtttccg
     8281 gggagaccct tccggcccgc cgatctcgat cggcggcgag ttgggggagt acatggccgg
     8341 cgcgtgcgcc gcctacggag cgctggcatt gcggcgacgc gtccgccgcg gcg
//
LOCUS       DASRJA010000689         2699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3153339,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000689 DASRJA010000000
VERSION     DASRJA010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3153339"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VFC01_30965"
     CDS             complement(<1..660)
                     /locus_tag="VFC01_30965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HZN84074.1"
                     /translation="MSVQLADIIDVLDAAYPPRLAQDWDSVGLVCGDPGETVETVTVA
                     VDATAAVVAEVPDRGLLLAHHPLLLRGVDTVAASTAKGALVHQMIRTGRALFTAHTNA
                     DSASPGVSDALADVLGLTVEEVLEPISVGAGLDKWIVFVPADSADAVRDAMSAAGAGS
                     VGTVERVPEDRVEVTAPTRLRAHVLAAMRAAHPNEEPAFDIYALATVPAGVGLGRVGL
                     LP"
     gene            complement(657..1679)
                     /gene="cobC"
                     /locus_tag="VFC01_30970"
     CDS             complement(657..1679)
                     /gene="cobC"
                     /locus_tag="VFC01_30970"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018599448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2231c family pyridoxal phosphate-dependent
                     protein CobC"
                     /protein_id="HZN84075.1"
                     /translation="MASQSRAAARYHGDQAAAPGMLDFAVNVRAAEPPSWLVDRLAAR
                     LADVGRYPSAADECRAVHAVAARHGRSVDDVVLLAGVAEGFALLANLRPRLAALIAPS
                     FTEPKAALAAAGVPFRHVVLSPPFGLAGACVPEEADLVVVGNPTNPTSVLHPRDEILA
                     LRRPGRILVVDEAFADAVPGEPESLAGEAFPDVVVLRSLTKTWALAGLRVGYAVGPAE
                     VLDRMAARRPQWPLGTLQLEAIAACCSPEAVAEAEGGARRLTTLRTAMVAGLTSIGLD
                     VIDGCAPFVLFTVPDAQLMRKHLDGKGIAVRRCDTFVGFDGEFLRAAIRPHWPVLADA
                     IVEALR"
     gene            1713..2390
                     /locus_tag="VFC01_30975"
     CDS             1713..2390
                     /locus_tag="VFC01_30975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HZN84076.1"
                     /translation="MTETVRSAVAARPQLVIFDLDGTLTDSAQGIVSSFRHALAAIGA
                     EVPDGDLASRIVGPPMHHTLREMGLGADADAAIAAYRADYTTRGWAMNRLFDGIDTLL
                     ADLRATGVRLAVATSKAEPTARRILAHFGLDGHFEVIAGASLDGSRATKSDVVAHALA
                     QLRPLPDRVLMVGDRSHDVEGAAEHGIDTVVVGWGYGRQDFADPHGVSASAHVFTIED
                     LREVLGV"
     gene            2383..>2699
                     /locus_tag="VFC01_30980"
     CDS             2383..>2699
                     /locus_tag="VFC01_30980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HZN84077.1"
                     /translation="MSEPLHITFICSGNICRSPMAEKMFAHQIKDRGLADAVRVSSAG
                     TGGWHAGDPADRRASHVLREHGYPTTHCAAQIADEHLAADLVVALGRNHVRMLRELGI
                     PPT"
BASE COUNT          444 a          986 c          900 g          369 t
ORIGIN      
        1 cggcaacaaa ccgactcggc ccagcccgac accggcgggg acagtggcca acgcatagat
       61 gtcgaacgcg ggctcctcat tgggatgtgc ggcgcgcatc gctgccagca catgcgcccg
      121 cagccgtgtc ggcgcagtca cctcgacccg gtcctcaggc acccgctcga cggtgccgac
      181 gctgcccgcg cctgccgcgg acatcgcgtc ccgcaccgcg tcggcgctgt cggcgggcac
      241 gaagacaatc cacttgtcca aaccggcgcc caccgagatc ggctccaaca cctcttcgac
      301 agtcagcccc aacacgtcgg cgagcgcatc cgagactccg ggcgacgccg aatcggcatt
      361 cgtatgggcc gtgaacaatg cccggccggt gcgaatcatc tggtgcacga gggcgccctt
      421 ggccgtgctg gcggcgacgg tgtcgacccc gcgcagcagc agggggtggt gcgcgagcag
      481 cagcccgcga tcgggcactt cggcgacgac ggcagcggtg gcgtcaacgg ccacggtgac
      541 ggtctcgacg gtttcgccgg ggtcgccgca gaccaggccc accgagtccc agtcctgtgc
      601 cagccggggc ggataggccg catcgagtac gtcgatgatg tcggctaact gcacgctcat
      661 cgcagcgcct cgacaatcgc gtcggccagc accggccagt gcggacgtat tgcggcgcgc
      721 aggaactcgc cgtcgaaccc gacgaacgtg tcacagcgcc gcacggcgat gcccttgccg
      781 tccaagtgct ttcgcatcag ttgggcgtcc ggcacggtga acagaacaaa gggtgcgcag
      841 ccgtcgatga catccaggcc gatgctagtc aatccggcca ccatcgcggt gcgcagtgtc
      901 gtcagccgcc gcgccccgcc ctcggcctcg gcgacggcct ccggtgagca acaggcggcg
      961 atcgcctcga gctgcagcgt gcccaacggc cactgcgggc gccgcgcagc catccggtcc
     1021 agcacctcgg cggggcccac cgcgtagccg acgcgcagcc cggccagcgc ccacgtcttg
     1081 gtcaggctgc gcagcaccac gacatcggga aacgcctcgc cggccaatga ctccggttca
     1141 ccggggaccg cgtcggcgaa cgcctcgtcg accaccagga tgcggcccgg cctgcgcaac
     1201 gccaggatct cgtcgcgagg gtgcagcacc gaggtcggat tggtcgggtt gccgaccacg
     1261 accaggtcgg cctcctccgg cacgcaggca ccggccagtc cgaacggcgg cgacagcacc
     1321 acatgccgaa acgggacacc ggccgcagcg agggccgctt tgggttcggt gaatgacggg
     1381 gcgatcagcg ccgccagccg gggacgcagg ttcgccaaca gcgcgaagcc ctcggccacg
     1441 ccggcgagca gcacgacatc gtccacgctt cgcccgtgcc tggcagcgac agcatgcacc
     1501 gcgcggcact catcggcggc gctcggatac cgtccgacgt cggccagccg ggccgccaac
     1561 cggtcaacca gccacgacgg cggctccgcg gcgcgcacat tgacggcgaa gtccagcatt
     1621 ccgggcgctg cggcctggtc gccgtggtag cgggcggcgg cacgggattg actggccact
     1681 ccacgacagt agacgtcggc gacaatggag tcgtgacaga aaccgtgcgc agtgccgtcg
     1741 cggcgcgccc gcagctggtg attttcgacc tcgacggcac cctcaccgac tcggcgcagg
     1801 gcattgtgtc cagcttccgg cacgcactgg ccgcaatcgg cgcggaggtg cccgacggtg
     1861 acctggccag ccgtattgtc gggcccccga tgcatcacac gctgcgcgag atgggtctcg
     1921 gagcggacgc cgacgctgcc atcgccgcct atcgtgcgga ctacaccacc cgcggctggg
     1981 cgatgaacag gctcttcgac ggcatcgaca cgctgctcgc cgacctgcga gccaccgggg
     2041 tgcggttggc ggtggctacg tcgaaggccg aaccgactgc gcggcggatc ctcgcgcatt
     2101 tcggactcga cgggcacttc gaggtcatcg ccggcgccag tctcgacggc tcacgcgcca
     2161 ccaaatccga cgtcgtcgcg catgcgctcg cccaactgcg cccgctgccc gaccgtgtgc
     2221 tgatggtcgg cgaccggtcg cacgacgtcg aaggcgccgc cgaacacggc atcgacaccg
     2281 tcgtggtcgg ctggggctac ggacgtcaag acttcgccga cccgcacggg gtgagcgcgt
     2341 cggcgcacgt cttcaccatc gaggaccttc gcgaggtgct cggtgtctga gcctctgcac
     2401 atcaccttca tttgctctgg caacatctgc cggtcgccga tggccgagaa gatgttcgcc
     2461 caccagatca aggatcgtgg cctggccgac gcggtgcgag tcagcagcgc cgggaccggg
     2521 ggctggcatg cgggcgatcc cgccgaccgg cgggcctcgc atgtgcttcg cgaacacggc
     2581 tatcccacca cccactgcgc cgcccagatc gctgacgagc accttgcggc cgacctagtc
     2641 gtcgcccttg gccgcaacca cgtgcggatg ctgcgcgaat tggggattcc cccaaccaa
//
LOCUS       DASRJA010000690         5220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_787659,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000690 DASRJA010000000
VERSION     DASRJA010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787659"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3207)
                     /locus_tag="VFC01_30985"
     CDS             complement(<1..3207)
                     /locus_tag="VFC01_30985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZN84078.1"
                     /translation="MGRDDEPDSLAAIFSADREIGRDLAAVDWARTPLGRPAGWPQSL
                     QTAVSILLSSRFSMWMAWGPDLTFFCNAAYRRDTLGRKYPWALGRPASEVWAEIWDDI
                     GPRIDWVLATGEATWDAALLLFLERSGYSEESYHTFSYSPLRDELGQVVGMLCVVSED
                     TARVIAQRRMATLRDLGSDPSVVRTEDETLAYAARQLGRNRRDLPFTLTYLVDDGGQA
                     RLAGWSGIASGHPAAPAVLPADGSAVWPLAEPARGDDVLVELDGDRYPNLPTGDWHEP
                     PTQALVMPLLRQGASPAGFLVAAINRYRSFDEDYRDFLTLVAGHIAAGIGSARSYQAQ
                     QRRAEELAELDRAKTTFFSNISHEFRTPLTLIMAPVAELRGNTSRVDETGRQELEVVY
                     RNSLRLAKLVNTLLDFSRIEAGRAQARYEPVDLSAVTAELASVFRSAIDGAGLAFTVD
                     CPPLGEPVYVDLDMWEKVVLNLLSNALKFTFDGSVSIAVRREDTHAVVTVSDTGIGVS
                     ASEMPRLFERFHRIEAARARSNEGSGIGLALVKELVALHSGSIDADSLEGEGTTFTVR
                     LPFGIAHLPADEIASVGREFAPSGIAEPYVAEALRWLPSEANTTPPPHEVLTADTAVG
                     NSFAVSGPQVRVLIADDNADMRQYLSRLLRSAGYVVDAVTDGRQALDAVRATLPDLVV
                     SDVMMPRLDGLALVAALRADTRTAAVPVLLLSARAGEEASIEGLQAGADDYLVKPFAA
                     AELVARVRANVELARLRSHHSRWRTALVDSLQEAFFVCDEHGVVIEINAAFTDILGYG
                     PELLPFEPTYPWWPDADTEPDANRQVSEAFATLLEHGHGSGTLPVNHRDGHRVWITFS
                     FNHTDDPETGRRVVVGTFRDVTAEHYLAQRETALGELNQQLAQADTLDDALSAAAEEL
                     RRVWQARRVLAATFAVGAADSDVAPDGAPQIDFAGEPTRWSELPVHVRQRLVSLRDGD
                     LLTTDTTEPGTAAIALQHPHGVLVLYLELDAQRPFTAEDSTLLTVLAGRVGQGLQRVY
                     QFDQQRETALALQNAILGPALLPNGFAVRYQPAARPLQV"
     gene            complement(3382..3741)
                     /locus_tag="VFC01_30990"
     CDS             complement(3382..3741)
                     /locus_tag="VFC01_30990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN84079.1"
                     /translation="METRQDVGDSAVVVAVAGDIDTATVDTLVKNLGTALNAALDHPR
                     RLLIVDLSDVTYFGSAGLNAVLGCYETGLAEGVAVRLVATAAEVIRPLQVTQLDAVIR
                     PYPTVPDAIAGPDDDRS"
     gene            complement(3762..4436)
                     /locus_tag="VFC01_30995"
     CDS             complement(3762..4436)
                     /locus_tag="VFC01_30995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS and ANTAR domain-containing protein"
                     /protein_id="HZN84080.1"
                     /translation="MATSDLAPSFRPGNPVGDGEPLNVGAFRFWFVGQRWEWSDEVAR
                     MHGYEPGAIVPTTELLLSHKHPDDRQHVQELLDHALHHGGSFSSRHRFIDTSGREHAV
                     IVLADRMLNDRGAVVGTEGYYIDLTDTVHQAGKKALDDALPELLEARAAIEQAKGALM
                     VVYRIDADQAFAMLQWRSQETNTKLRSLAAQIVAELDTVQYQPDTLRRQFDHLLLTVH
                     KRIPTR"
     gene            4707..>5220
                     /locus_tag="VFC01_31000"
     CDS             4707..>5220
                     /locus_tag="VFC01_31000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HZN84081.1"
                     /translation="MGHAVAGVLVVLALGAATRFLETQVPQWTTGTPFAKVAKSIEFP
                     VYAIALGLIGNAALSKLALRDALSKGFRTEFFIKTGLVLLGASINLKVLVTAAGPAII
                     QALLLISIVFGFTWWLGGRLGLEDKLRALLASAVSICGVSAAIAAAGAVQAKREQLAY
                     AASLVIAFALP"
BASE COUNT          894 a         1699 c         1718 g          909 t
ORIGIN      
        1 cacctgcagc ggccgcgcgg ccggttgata tcgcaccgca aatccgttgg gcaacaacgc
       61 cggtccgagt atcgcgttct gcagggccaa cgcggtttcg cgctgttgat cgaactgata
      121 cacgcgctgc agaccctggc cgacgcgccc tgcgagcacc gtcaacaggg tgctgtcctc
      181 ggcagtgaac gggcgttgcg cgtccagctc gaggtacagc accaggactc catgcgggtg
      241 ctgcagcgcg atcgccgccg tccccggttc tgtcgtgtcg gtggtcagca ggtcgccgtc
      301 gcgcagcgaa accagccgct gacggacgtg tacgggcaac tctgaccacc gcgttggctc
      361 accggcgaag tcgatctgcg gtgcgccgtc tggggccacg tcggagtctg ctgcaccgac
      421 ggcgaaggtg gctgccagca cgcggcgggc ctgccagacg cggcgcagct cctcggccgc
      481 ggcgctcagc gcgtcatcga gggtatcggc ttgagcaagt tgctgattca gctcgcccag
      541 cgcggtctca cgctgggcga gataatgctc tgcggtgacg tcgcggaatg tgccgaccac
      601 gacgcgacgc ccggtctcgg gatcatcggt gtggttgaaa ctgaacgtga tccacactcg
      661 atgtccgtcc cgatggttga cgggcagggt gccgctgccg tggccgtgct ccagcagcgt
      721 ggcgaaagcc tcgctgactt gccggttggc gtcgggttca gtgtcggcgt cgggccacca
      781 cgggtaggtc ggttcgaacg gcaataactc tgggccgtac cccaagatgt cggtgaacgc
      841 agcattgatc tcgatgacca cgccgtgctc gtcgcagacg aagaacgctt cctgcaatga
      901 atcgacaagc gctgtgcgcc agcgcgaatg gtggctgcgc agccgtgcca actccacatt
      961 ggcacggacg cgagcgacca actccgcggc agcgaacggc ttgacgaggt agtcgtcggc
     1021 acccgcctgt aatccttcga tggaggcttc ttctcccgcg cgcgccgata gcagcagaac
     1081 tggtaccgcg gcggtgcggg tgtccgcccg gagcgccgcg acaagggcga ggccgtcgag
     1141 tcggggcatc atcacatcgc tgacgacgag gtcgggcagg gtcgcgcgga ccgcgtcaag
     1201 ggcttgccgg ccgtcggtca ccgcgtcgac gacatagccg gcgctgcgca gcagccgcga
     1261 caagtattgg cgcatatcgg cgttgtcgtc tgcgatcaaa acccgcactt gcggaccgga
     1321 cacggcgaag gagttgccca ccgcggtatc cgcggtcaat acctcatgcg gcggcggtgt
     1381 ggtgttggcc tcgctgggga gccatcgcaa cgcttcggcc acatacggtt cggcgatgcc
     1441 ggatggcgcg aattcgcgcc ctactgaggc gatctcgtcg gcgggcagat gcgcgatacc
     1501 gaacggcagg cggacggtga atgtggtgcc ctccccttcg aggctgtcag cgtcgatgct
     1561 tccactgtgc agcgcgacca gttctttgac gagggccaag ccgatgccac tgccctcatt
     1621 ggagcgggcg cgggccgcct cgattcggtg aaagcgttcg aacaaccgcg gcatctccga
     1681 ggcggacaca ccgattccgg tgtcgctgac cgtgacgacc gcgtgggtgt cctcccggcg
     1741 cactgcgatc gagacactgc cgtcgaaggt gaatttcaac gcgttggaga gcaggttgag
     1801 caccaccttc tcccacatgt ccagatccac gtacaccggt tcacccaacg ggggacagtc
     1861 cacggtgaat gccagtcccg ccccgtcgat ggctgaccgg aaaacgctgg ccagctcggc
     1921 ggtgaccgcc gacaggtcca ccggctcgta acgagcctgg gcccggcctg cctcgatgcg
     1981 ggagaagtcc agcagtgtgt tgaccagttt ggccagccgc agcgagttgc ggtagacgac
     2041 ctcgagttct tggcggcccg tctcgtcgac ccgagatgtg ttgccgcgca gctcggctac
     2101 tggggccatg atcagcgtca gcggggtacg gaattcgtga ctgatgttcg agaagaacgt
     2161 tgtcttggcg cggtcgagct cggcgagttc ctcggcgcgc cgctgctggg cctgataact
     2221 acgcgcactg ccaatgcccg ccgcgatgtg tcccgcgacc agcgttagga agtcgcggta
     2281 atcctcgtcg aacgagcggt agcgattgat cgcggccacc agaaaccctg ccggtgaagc
     2341 gccctgccgc agcagcggca tcaccagcgc ctgcgtcggc ggctcatgcc aatcaccagt
     2401 cggtaggttc gggtagcggt cgccatccag ctcgacgagc acgtcatcac cgcgcgcagg
     2461 ctcggccagc ggccacaccg ccgacccgtc cgcaggcagc accgcggggg cggcgggatg
     2521 cccggacgca atcccgctcc atcccgcgag ccgcgcctgg ccaccgtcat cgacgagata
     2581 ggtcagcgtg aacggcaggt cccgccgatt acggccaagc tggcgggcgg cgtacgccag
     2641 cgtctcgtcc tcggtgcgca ccacactcgg atccgagccg agatcgcgca gtgtcgccat
     2701 ccggcgttgc gcgatcactc gtgccgtgtc ctcgctgacg acgcacagca tgccgaccac
     2761 ctggccgagt tcgtcgcgca gcggactata ggagaacgtg tggtaggact cctccgaata
     2821 gcccgaccgt tccaggaaca gcagcagcgc cgcatcccag gtggcctcgc cggtggcgag
     2881 cacccaatcg atgcgtgggc cgatgtcgtc ccagatttcg gcccacacct cactggccgg
     2941 tcgtcccaac gcccacggat atttgcggcc cagagtgtcg cggcggtagg cggcgttaca
     3001 gaagaacgtc aggtccggtc cccatgccat ccacatcgaa aaccgcgacg acagcaggat
     3061 actgaccgcc gtttgcaggc tttgcggcca acccgccggt cgtccgagcg gcgtgcgtgc
     3121 ccaatcgaca gcggcgagat cgcgaccgat ctctctatcc gcgctgaaga tggcggccag
     3181 cgagtcgggt tcgtcgtccc tgcccatcgg aactccccac gtcactggct cgccccgaag
     3241 ggagtggggc agcggtgatc ctcgtgtttc aacagccgtg caacggtgat tgcaagagcc
     3301 gatataccca ccccggcggg ctgtcactcc ccgattcggg ctagatatcg cggccacgcg
     3361 cagccggctt cgagcgcgca atcagctccg gtcgtcgtcc ggacctgcta tggcgtccgg
     3421 cacggtggga taaggccgaa tgaccgcgtc gagttgggtc acttgaaggg ggcgtatcac
     3481 ttcggcggcg gtggccacca accgcaccgc gacgccctcg gcgagcccgg tctcataaca
     3541 tccgaggaca gcgttgaggc ccgcgctgcc gaaataagtc acgtcgctca agtcgacgat
     3601 cagcagcctc cgcggatggt ccaaagccgc attgagtgcg gtgccgaggt tcttgaccaa
     3661 cgtgtcgacg gtggccgtgt cgatatcacc cgcgactgca accacaaccg cggagtcgcc
     3721 gacatcctgc ctggtttcca acgtcgccaa ccctatccgg cttagcgcgt tggtatgcgc
     3781 ttatgaacgg tgagaagcag gtgatcgaac tgccgacgca gggtgtctgg ttgatattgc
     3841 accgtgtcga gttcggcgac tatttgtgcg gcaagtgacc gaagcttggt gttcgtctcc
     3901 tgcgagcgcc actgcagcat cgcgaacgcc tggtcggcgt cgatacgata aacgaccatc
     3961 aaggcgccct tcgcctgttc gatcgccgcg cgcgcctcca agagttcggg cagggcgtcg
     4021 tcgagcgcct tcttacccgc ctgatgaacc gtgtcggtca ggtcgatgta atacccctcg
     4081 gttccgacga cggcgccgcg gtcgttcagc atccggtcgg cgaggacgat gaccgcgtgc
     4141 tcacgacccg aggtgtcgat gaaccgatgc cggctggaga aggacccgcc gtgatggagc
     4201 gcatgatcga gaagttcttg gacgtgctgc cggtcgtcgg gatgcttatg ggaaagaagc
     4261 agctcggtcg tcggcacgat cgcgcctggc tcatagccgt gcatgcgagc cacttcatcg
     4321 gaccattccc accgctggcc cacgaaccag aagcggaagg cgccgacgtt caacggctcg
     4381 ccgtctccga cgggattgcc cggtcggaag ctgggcgcca gatccgatgt ggccatgctc
     4441 tatcccacca caaagtgcga tgtgcggtgg gcggtctgag ttcggttcgc gctgatcgca
     4501 acggatgacc ttgaaatcgg ctgtgaatac gttgcgcgca tgaccgatga gccccgtggg
     4561 cgaagagtaa cgatcggcga agactggctg gccgtcatcg tcggccttgc gctactggcc
     4621 ctcgtactcc tcggcgcgat ccctggcagc gtgatcccga gacggaaccg accaccgaga
     4681 cgaagccgga cgcctcgtcg tcgggcatcg gccatgcggt cgccggtgtg ctcgtggtcc
     4741 tcgcgctcgg cgcagcaacc cggttcctgg agacacaggt gccgcagtgg acgacgggca
     4801 cgccgttcgc caaggtcgcg aagtcgatcg aattcccggt ctacgcaatc gctttgggct
     4861 tgatcggcaa cgccgcgttg agcaagctgg cgctgcgcga cgccttgtcg aaaggattcc
     4921 gcaccgaatt cttcatcaag accggcctgg tgctgttggg tgcgtcgatc aacctcaagg
     4981 tgctcgtcac cgcggcggga ccggcgatca tccaggcgct gctgctgatc tccatcgtgt
     5041 tcggcttcac gtggtggctc ggcgggcggc tcggactcga ggacaagctg cgcgccctgc
     5101 tggcatcggc agtatcgatt tgcggtgtca gcgcggcgat tgcggcggcc ggcgccgtgc
     5161 aagccaagcg tgaacaactg gcctacgccg cgtcgttggt tatcgccttc gcgctgccat
//
LOCUS       DASRJA010000691         6026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1261925,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000691 DASRJA010000000
VERSION     DASRJA010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261925"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFC01_31005"
     CDS             <1..582
                     /locus_tag="VFC01_31005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZN84082.1"
                     /translation="RMVGRRMYGGTSTYLPLKVNQVGVIPVIFASSLIYIPHLITQLI
                     RSGSGGQGNSWWDRFVGNYLTNPADPVYIAIYFGLIIFFTYFYVSITFNPEERADEMK
                     KFGGFIPGIRPGKPTADYLRYVLSRITLPGSIYLGVIAVLPNLFLEIGNTGAVQNLPF
                     GGTAVLIMIGVGLDTVKQIESQLMQRNYEGFLK"
     gene            579..1127
                     /locus_tag="VFC01_31010"
     CDS             579..1127
                     /locus_tag="VFC01_31010"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZN84083.1"
                     /translation="MRVVLLGPPGAGKGTQAQKLSEKLGIPQISTGDLFRKNISEGTP
                     LGVEAKRYLDAGDLVPSELTNKLVEDRIEQPDATDGFILDGYPRSVEQAKALDEMLKN
                     HDTKLDVVLEFAVSEQELLERLKARGRADDTEEVIHNRMQVYRDETEPLLEYYSHNNL
                     QTVEAVGALDEVFARALRALGR"
     gene            1157..1954
                     /gene="map"
                     /locus_tag="VFC01_31015"
     CDS             1157..1954
                     /gene="map"
                     /locus_tag="VFC01_31015"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZN84084.1"
                     /translation="MIGLPGLRNRKVVAQRSAGELDAMEAAGSLVARALLAVRQAAAP
                     GVSTLALDEIAESVVRDGGGVPSFLGYHGFPASICSSVNDRVVHGIPSATETLAPGDL
                     VSIDCGAILDGWHGDSAITFGIGTLIPVDEALSAATREAMEAGIAAMLPGNRLTDVSH
                     AIEAETHAAEDRYDRKFGIVAGYGGHGIGRQMHMDPFLPNEGPPGRGPYLAPGSVLAI
                     EPMLTLGTTKTVVLEDEWTVVTADGSRAAHWEHTVAVTDDGPRILTL"
     gene            2207..2701
                     /locus_tag="VFC01_31020"
     CDS             2207..2701
                     /locus_tag="VFC01_31020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN84085.1"
                     /translation="MMRVLYDEHAAALWRYALRLTGDQARAEDVVQETLLRAWRHPEV
                     TDDVERSARAWLFTVARNLIIDERRSARYRNESGTSDLEQVADRAAPDEVDSALDRLL
                     LSTALSQLSEEHRAVIRRSYYQGWTTAQIANDLQIAEGTVKSRLHYAVRALRLNLQEM
                     GVTR"
     gene            2698..3438
                     /locus_tag="VFC01_31025"
     CDS             2698..3438
                     /locus_tag="VFC01_31025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZN84086.1"
                     /translation="MTQFGFPLGGDLLDGDRYVTWDAAYVLGSLTSNERHEYQAHLET
                     CKRCRSAVAEISGIPALLAMLDLEDVRALDEETSETPPLRPEVLDSVLDKVRWRRRRS
                     RWLTSAAVGVAAALLAVGAVIAIRPEIVGLENYTPEQTTQAMEMAKVSDTPINASISL
                     TGFGWGTRIDMACSYGSWGQAGTIPPQNLGMVVVGRDGSHTQIATWLGLSGATALPSA
                     TTPMQKDEIAAVQLVSSENGKVLLEKNL"
     gene            3511..5241
                     /locus_tag="VFC01_31030"
     CDS             3511..5241
                     /locus_tag="VFC01_31030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZN84087.1"
                     /translation="MARRHRVVDHIVAYLAASGVDYFFGVDGANIEDLYDAAFFRDDI
                     TAVLAKHEFSAATMADGFSRSGAGLGVVAATSGGGCLNTVPGLGEAYASRVPVLALIG
                     QPPTTLDGKGSFQDTSGRNGALNAEALFSAVSVYCRRVVRPGDIVTALPEAIAAARTG
                     GPAVLLLPKDIQQAETGPNGVGFEPAATQQGDPGVIAYALRGVDGPITIIAGEQIARD
                     GARVELEQLRAMLRARIATVPDAKDVAGTPGMGSSSSLGVAGVMGHPGVAAALADSAL
                     CLLVGTRLSVTSRAGLDEALAAVPVVSIGSAAPYVACTHVHTADLRGSLSMLIAALSG
                     HGRPLGVRVPDAVPHTELRPPRLSGPGVRYRDAMTVLDAALPDGTDIVVDAGNIGASA
                     IHYLPVRRDGRFIVALGMGGMGYSFGAGVGVAFGRASARANGDRVRRRTVVIAGDGSF
                     LMHGMEIHTALQYRLPVTFLLFDNHAHAMCVTREQLFYDDLYSYNRFGPSRFGAGLAT
                     MFPGLQSVDVDEIGQLSDAVRTALDVDGPSVVSVECSADEIPPFANFLGAAAKPSISK
                     ESRSNVTTRA"
     gene            5222..5887
                     /locus_tag="VFC01_31035"
     CDS             5222..5887
                     /locus_tag="VFC01_31035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN84088.1"
                     /translation="MSLPALDDITAHRGTIEPIDDLVRIETSPREKATPIIMEMMRSV
                     YPHDEVFGEFCTVNSYIDCPPDELFDYMADTRCLEEWTYSLRGFTPTEEPGLWLAFDR
                     LGSETKIFTRTVANRDAMTVDYHCAWDQGKHLWMIYLMRIVDAQMVFDKPGSVVLWTN
                     CHHPFYDHNGYPETAPPERPVWVGDFWDMFGPGHLLELKNLKAIAEYRHHNGLPVTPA
                     WMR"
     gene            5895..>6026
                     /locus_tag="VFC01_31040"
     CDS             5895..>6026
                     /locus_tag="VFC01_31040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HZN84089.1"
                     /translation="MTVSLVDVSTYLPGEPIGAEYYAQFAESDDLRDNVMFRAPKFRH
                     "
BASE COUNT         1076 a         1926 c         1977 g         1047 t
ORIGIN      
        1 cgcatggtgg gtcgacgcat gtacggcggc acgtcgacgt atctgccgct caaggtcaac
       61 caggtcggcg tcatcccggt catcttcgcg tcgtcgctga tctacattcc gcacctgatc
      121 acgcagctga ttcggagcgg cagcggcggt caaggcaaca gctggtggga ccggttcgtc
      181 ggcaactacc tgaccaaccc cgccgacccg gtatacatcg ccatctactt cgggctgatc
      241 atcttcttca cgtacttcta cgtgtcgatc acgttcaatc ccgaggaacg cgccgacgag
      301 atgaagaagt tcggcggctt catccccggc atccgccccg gcaaacccac cgccgactac
      361 ttgcgctatg tgcttagccg gatcaccctt ccaggctcga tctatctggg cgtgatcgcc
      421 gtgctgccga acctgttcct cgaaatcggc aacacgggcg ccgtgcagaa cttgccgttc
      481 ggcggtactg cggttctgat catgattggc gtcggattgg atacggtcaa acagatcgag
      541 agccagctga tgcaacgcaa ctacgaaggg ttcctgaagt gagagtcgtt ctactcggac
      601 cgcctggcgc gggcaaaggc actcaggctc agaagctgtc cgagaagctc ggcattccgc
      661 agatctcgac gggggacctg ttccgcaaga acatcagcga gggcaccccg ctgggtgtcg
      721 aggccaagcg gtacctcgat gcaggcgatc tggtgccgtc ggagctgacg aacaagctgg
      781 tcgaagaccg gatcgagcag cccgatgcca ccgacggctt catcctcgac ggttaccccc
      841 gctcggtgga acaggccaag gcgctcgacg agatgctgaa gaaccacgac accaagctgg
      901 acgtcgtgct ggagttcgcc gtctccgagc aggagctgct cgagcggctc aaggcccgtg
      961 gccgcgccga cgacaccgaa gaggtcatcc acaaccggat gcaggtgtac cgcgacgaga
     1021 ccgagccgct gctggagtac tacagccaca acaacctgca gacggtcgaa gccgttggcg
     1081 cgttggacga ggtgttcgcc cgtgccctca gggcgctcgg tcggtgaacc gaccgttcaa
     1141 cgggagcaaa tagtcgatga tcggccttcc ggggctgcgc aaccgcaagg tcgtcgcgca
     1201 gcgtagcgcg ggtgaactcg atgcgatgga agcggccggg tcgctggtcg cgagggcgct
     1261 tctggccgtc cggcaggcgg cggcgcctgg ggtatcgacg ctggcactcg atgagatcgc
     1321 cgaatccgtc gtccgcgacg gtggcggtgt gccgtcgttc ctcggctacc acggcttccc
     1381 ggcgagcatc tgctcatcgg tgaatgaccg tgtggtgcat gggattccgt ctgcaaccga
     1441 aaccctcgcg ccgggagatc tggtatccat cgactgcggg gcgatcctcg acggctggca
     1501 cggcgactcg gcgatcacgt tcggcattgg aacgctgatc cccgtcgacg aagcgctgtc
     1561 cgcggccacc cgggaggcaa tggaagccgg aatcgccgcg atgctgcccg gcaaccggct
     1621 caccgacgtc tcgcacgcca tcgaggccga aacgcacgcg gccgaggacc gttacgaccg
     1681 caagttcggc atcgttgccg gctacggcgg ccacggcatc ggcaggcaga tgcatatgga
     1741 ccctttcctg cccaacgagg gtccccccgg acgcgggcca tacctcgcgc ctggctcggt
     1801 gcttgccatc gaacccatgc tcaccctcgg caccacgaag accgttgtgc tggaggacga
     1861 atggaccgtc gtcaccgccg acggatcccg cgccgcgcac tgggagcaca ccgtcgccgt
     1921 gacggacgac gggccgcgaa ttctcacgct gtagtacgcg taactctccg ccgaacgtgg
     1981 gttaccggca cgtcgcaggc ctccaaggcg tgtcttaagc ccacattcgg tgctcgccgt
     2041 catgaagatt gcttgtacgg tcgtgcactg cgctgaaatc acagccatgg gcgcaatctt
     2101 caccattcgg gctctgccca ggccacgggc ggcgtagtaa tgaacccgaa caccaccaac
     2161 gtcgtatcta agacggaggt tggtagtgga cgatcccgag gccgccatga tgcgggtgct
     2221 ctatgacgag cacgccgctg cactgtggcg ttatgccctg cggctaactg gggatcaagc
     2281 gcgtgcggag gacgtagtgc aggagacgct gcttcgggca tggcgccatc ccgaggtgac
     2341 cgacgacgtc gagcggtcgg ccagggcgtg gctgttcacc gtggcgcgta acctgatcat
     2401 cgacgagcgt cgcagcgcgc gataccgcaa cgaatccggc acatccgacc tcgaacaggt
     2461 cgccgaccgt gccgcacccg acgaggtgga ctccgcattg gaccgattgc tgctgagcac
     2521 ggcgttgagc cagctatcgg aggagcatcg tgcggtgatt cgccggtcct actaccaggg
     2581 ctggacgacc gcacagattg ccaatgacct acagatcgcg gagggcacgg tgaaatcccg
     2641 gcttcactac gcggttcggg cgttgcgact caatctgcag gaaatggggg tgacacgatg
     2701 acacaattcg gttttcccct aggcggtgat ctgttggacg gcgatcgcta cgtgacgtgg
     2761 gacgcggcct atgtcctcgg ctcactgaca agcaatgaac gccacgagta ccaggctcac
     2821 ctggaaacct gtaagcggtg ccgatcggcg gtcgcggaga tcagcggcat cccggcgctg
     2881 ctggcgatgc tcgacctcga ggacgtgcgc gcgctggatg aggagacgtc cgaaaccccg
     2941 ccgcttcggc ccgaggtgct cgattcggtc ctggacaagg ttcggtggcg gcgccgtcgg
     3001 tctcgctggc tgacctcggc cgcggttggg gtagccgccg cgttgctggc agtcggggcg
     3061 gtgatcgcca ttcgtcccga gatcgtgggg ctggagaatt acaccccgga gcagaccacc
     3121 caggcgatgg agatggccaa agtttcggat actccgatca acgcgtcgat ctcactgacc
     3181 ggttttggct ggggcacccg gatcgacatg gcctgctcct acggaagctg gggtcaggcc
     3241 ggcaccattc ctccgcagaa cctgggaatg gtcgtggtgg gccgcgacgg cagccacacc
     3301 cagatcgcaa cgtggctcgg attgtcaggt gcgactgcgc tgccgagtgc caccactccg
     3361 atgcagaagg acgaaatcgc tgcggtgcaa ctggtttcgt cagaaaacgg aaaggtcttg
     3421 ctcgagaaga acctgtgatc cgcgccaccc agtgaaccca acttcgttgc gagtcgtgtc
     3481 actggtgggg cggggagaag cgggtgagaa atggccagaa ggcatcgagt tgtcgatcac
     3541 atcgtcgcct accttgcggc gagcggcgtc gactacttct tcggtgtcga cggtgccaat
     3601 atcgaggatc tttacgacgc cgcattcttc cgtgatgaca tcaccgccgt cctggccaag
     3661 catgagttct ccgcggccac catggccgac ggcttcagcc gcagcggtgc cgggctcggc
     3721 gtggtcgccg cgacttccgg cggcggctgc ctgaatactg tgcccgggct gggcgaagcg
     3781 tacgcgagcc gggtgccggt gctggcgttg atcggtcagc caccgaccac actcgacggc
     3841 aagggcagct tccaggacac cagtggtcgc aacggcgcgc tcaacgccga ggcgttgttc
     3901 tccgcggtgt cggtgtattg ccgtcgcgtg gtgcggcccg gggacatcgt cacggcgctg
     3961 cccgaggcta tcgctgccgc gcgcaccggc ggacccgcag tgttgttgct gcccaaggac
     4021 attcagcaag ccgagaccgg gcccaacggc gttgggtttg aaccggccgc gacacagcag
     4081 ggcgaccccg gggtgatcgc gtatgccctg cgcggcgtcg acgggcccat cacgatcatc
     4141 gccggtgagc agatcgctcg tgatggcgcg cgcgtcgaac tcgagcagct gcgggcgatg
     4201 ctgcgcgccc ggatcgccac ggtgcccgac gcgaaggacg tcgcgggcac accaggcatg
     4261 ggatcgtcgt cgtctctggg tgtcgccggc gtaatgggcc acccaggcgt ggcggccgcc
     4321 ctcgcggaca gcgccttgtg cctcctggtg ggcacccgcc tgagcgtgac gtcacgcgcc
     4381 gggctggacg aggcgctggc ggcggttccc gtggtgtcga tcggatcggc ggcgccctac
     4441 gtggcatgca cccacgtgca cacagccgat ctgcgcggct ccttgtcgat gttgatcgcg
     4501 gcattgtcgg gtcacggcag gccgctcggg gtgcgggtgc ccgacgcggt gccgcacacc
     4561 gaattgcggc cgccgcggct cagtggtccc ggggtccgct accgggatgc gatgaccgtg
     4621 ctcgacgcgg cgctgccgga cggcaccgac atcgtcgtcg acgcgggaaa catcggcgca
     4681 tcggcgattc actacctgcc ggtgcgacgc gacggccgct tcatcgtcgc gctcggcatg
     4741 ggcggcatgg gatacagctt tggagccggc gtcggcgtgg cgttcgggcg agcgagcgct
     4801 cgcgccaacg gcgaccgagt caggcggcga acggtcgtca tcgccggcga cggctccttc
     4861 ttgatgcacg gcatggagat tcacacggca ctgcaatacc gcctgccggt gacgttcctg
     4921 ctcttcgaca accatgcaca cgcgatgtgc gtgacacgcg agcagctgtt ctacgacgac
     4981 ctgtacagct acaaccggtt cgggccaagc cgtttcggcg ccgggctggc cacgatgttc
     5041 cccggcttgc agtcggttga tgtcgacgag atcggccagt tgtccgacgc tgtgcgcacc
     5101 gcgctggacg tcgacggccc gtcggtcgtg agtgtcgagt gctcggccga cgaaatcccg
     5161 ccgttcgcaa actttctcgg cgccgccgcg aaaccctcga tatctaagga gagccgctcc
     5221 aatgtcacta cccgcgcttg atgacatcac cgcacaccgg ggcacgatcg aaccgatcga
     5281 cgatctggtg cgcatcgaaa cgtcgcctcg ggagaaggcc acgcccatca tcatggagat
     5341 gatgcggtcg gtgtacccgc atgacgaggt gttcggtgaa ttctgcacgg ttaatagcta
     5401 tatcgactgt ccgcccgacg agttgttcga ctacatggcc gacacccgct gcctcgaaga
     5461 gtggacgtac agcctgcgcg ggttcacgcc gaccgaggaa cccggtctgt ggctagcctt
     5521 tgaccgatta ggttcggaga caaagatctt cacccgtacg gtggccaaca gggacgccat
     5581 gacagtcgac taccactgcg catgggacca gggcaagcac ctgtggatga tctacctgat
     5641 gcggatcgtc gacgcgcaga tggtgttcga caagccgggc tcggtcgtgc tgtggaccaa
     5701 ctgccatcac ccgttctacg accacaacgg ttacccggaa accgcgccgc ccgagcggcc
     5761 ggtgtgggtc ggcgatttct gggacatgtt cggccccggc catctgcttg agctgaagaa
     5821 cctcaaggcg atcgccgaat accgccacca caacggcctg ccggtcaccc cggcctggat
     5881 gagatgagaa tgttgtgaca gtcagcttgg tcgacgtgtc gacatacctg ccgggggaac
     5941 cgatcggcgc cgagtactac gcccagttcg cggagtccga cgacctgcgc gacaacgtga
     6001 tgttccgcgc accgaagttc cgccac
//
LOCUS       DASRJA010000692        16296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1462717,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000692 DASRJA010000000
VERSION     DASRJA010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 16296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16296
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462717"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..647
                     /locus_tag="VFC01_31045"
     CDS             54..647
                     /locus_tag="VFC01_31045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZN84090.1"
                     /translation="MPRSRVTGVVLAAGNSRRLGTPKQLLPFDETTVLGATIEVARGC
                     PFDQLIVTLGGAATAVREAVNLEGIDTVIADDHESGCSSSLRAALSRVDLRAEGIVVM
                     LGDQPGVSREAVAKLIDSGRGAPIAVCRYSDGIGHPFWLGRPVFGEVAELHGDKGVWR
                     LIESGRFDVRQVPTDGTVPLDVDTWDDYQRLLGSVAR"
     gene            644..1552
                     /locus_tag="VFC01_31050"
     CDS             644..1552
                     /locus_tag="VFC01_31050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZN84091.1"
                     /translation="MTEPVAGLFSDVDEVISRFDAHDYLLDAGTASAIYLAAVLGRPL
                     LLEGEPGVGKTTAAKTLAAVLDAPLLRLQCYEGLTAAEALYDWNYQRQLLSIRLAEAR
                     GEAIGESDLYTETYLVDRPILQCVRYRGPGAPVLLIDEIDRADDEFEALLLEFLGEAA
                     VTVPELGTFVAQRPPIVVLTSNRSRDLHDALRRRCLYHWIDYPDPVRAAAIVRRTVPG
                     ATAALIQHAAQFVGETRVLDMDKPPGIAETIDWVAALVALGVADLVAGDARTSAVVSL
                     GALAKTPDDRGVVAEAFADYMEGICR"
     gene            1549..2208
                     /locus_tag="VFC01_31055"
     CDS             1549..2208
                     /locus_tag="VFC01_31055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN84092.1"
                     /translation="MKITNEFTVSAPAEKAWEVLTDLQQVVPLMPGAQLVGQEGDDYL
                     GKVKVKVGPVTSEFSGKAHFVERDGKTHRGVVDARGKEARGTGNAAATITFGVHEAGD
                     RSRVTVDTDLKIVGKLAQFGGGMLQQVSEKLLGEFVDSLEAKLAAGDAEEDAARTAPT
                     NGQVARPAPEPAPIDLIELAGGSAIKNYGPAVFGAFLLAAVVFTLGRLRIARKALRGK
                     R"
     gene            2205..3395
                     /locus_tag="VFC01_31060"
     CDS             2205..3395
                     /locus_tag="VFC01_31060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZN84093.1"
                     /translation="MTAPFLLRGVDLAAFAVALVNRLHSGGVVVSASGPTGFVDALRR
                     NWPRTRSQLYWVARLTLVNRVEDLAAFDAVFESVFADAVLGVDPPGLKQSMGTAAAVH
                     PGVPGRASTLADGGLPWTTRPASVTAVDARTADIGIPDALPSRIVARADEPFERFDPD
                     DLRLLGEWLEQAAAHWPRRRSQRRELHRHGKRVDLRETMKASRATGWETLRVARTRNR
                     EYPRRVVLVCDVSRSMQPYATIYLHLMRAAALRQNGIRPEVFAFSTSLTRLTAALSHR
                     SPEVALAKANAKVTDRYGGTHLGRCVGELVSASHGNALRGAVVIIASDGWDSDPPDVL
                     ERALIRVRRRAQKLIWLNPRSAQPDFRPLAASMAAALPYCDVFMPAHSVTGLRELFAV
                     LADQ"
     gene            complement(3404..4102)
                     /locus_tag="VFC01_31065"
     CDS             complement(3404..4102)
                     /locus_tag="VFC01_31065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZN84094.1"
                     /translation="MDTTTAVSAAATQLALMPDFLDPIKLIGYFGTWALVGLLVVIFV
                     ESGVLFPVLPGDTLLFVAGMLAAGTAAAGQANFKLWQLLVFIPIAAILGGQVGYFIGR
                     YLGTAMFKPNARFLKQRYLDEAHIFFEQRGPFAIVLARFVPIVRTLAPITAGAARMGY
                     GAFTLFNVIGAVVWGVGLTLLGYGLGQFEIIQKLLEPIFILIALVSVMPMFIEWYKRR
                     RAAKRTAGEPAAEA"
     gene            4162..5178
                     /locus_tag="VFC01_31070"
     CDS             4162..5178
                     /locus_tag="VFC01_31070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZN84095.1"
                     /translation="MATTHRAVHVASANAPLTLVDVETTPPGPGHVRITVAACGVCGT
                     DHGFVAGGFPNMTWPLTLGHELAGTIAELGDGVEGFAVGDRVAVGWFGGNCNRCIPCR
                     QGKFMQCENGEVPSWHYPGGYAESVTAPATALARIPDALSFVEAAPMGCAGVTTYHAL
                     RKTKAVAGDRVAVLGIGGLGHLGVQWARAMGFETIAIARGADKEANARELGAHHYIDS
                     RAEDVPAALQALGGASVVLATAANSAAMAQTVGGLAPEGELVIVGVTADPLPIAPLQL
                     ISPGLSVSGHPSGTSRDIEETMHFAVLSGVRAWIEERPLAEAAEAYAAMDEGRARYRM
                     VLTM"
     gene            5235..6272
                     /gene="fbaA"
                     /locus_tag="VFC01_31075"
     CDS             5235..6272
                     /gene="fbaA"
                     /locus_tag="VFC01_31075"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HZN84096.1"
                     /translation="MPIATPEVYAEMLGRAKENSFAFPAINCVGSESINAALKGFADA
                     GSDGIIQFSTGGAEFGSGLGVKDMVTGAVALAEFAHIVADKYPITVALHTDHCPKDKL
                     DSYVTPLLAISQKRVAEGRNPLFQSHMWDGSAVPIDENLDIARELLKEASAAKIILEI
                     EIGVVGGEEDGVAHEINDKLYTTPEDFEKTIDALGAGEHGRYLLAATFGNVHGVYKPG
                     NVKLRPDILEQGQKVAAAKLGLPEGAKPFDFVFHGGSGSLKSEIEDSLRYGVVKMNVD
                     TDTQYAFTRPLAAHMFTNYDGVLKVDGEVGNKKAYDPRSYLKKAEASMSERVVEACKD
                     LQSAGRSVSGG"
     gene            6283..6567
                     /locus_tag="VFC01_31080"
     CDS             6283..6567
                     /locus_tag="VFC01_31080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84097.1"
                     /translation="MKRGILIACCTVAIPMGTLAVAAPAGANTCPWGTVPRFDGVCTQ
                     GQSGGTPPPGLTVPPQGADIVTMPGQFPTVNGIPCTDEHFGTCYAMSQNG"
     gene            complement(6564..7373)
                     /locus_tag="VFC01_31085"
     CDS             complement(6564..7373)
                     /locus_tag="VFC01_31085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HZN84098.1"
                     /translation="MSNPSGPDDPDEATEPAAEAVVETDETQPAEAVTDPEHEPATEI
                     LLSPEAQAQLPAEETDKPAERRFTAPSGFDGSTQKIDTPAEPATEVMPPVPTEAMPQA
                     KPVAPQVIPPRTDPPKPPRNRRSWGWVVALLLVIAALVAVAILGTVLLTRGSTPKVSE
                     EDKVRTTIQNFDVAVQNGDLATLRSITCGTTRDNYVNYDDRAWTDTHARVAAAKQYPV
                     VASIDQVVVNGDHAEANVTTFMAYAPQTRSTRSFDLQFRDDQWKICQAPDS"
     gene            7433..7846
                     /locus_tag="VFC01_31090"
     CDS             7433..7846
                     /locus_tag="VFC01_31090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 domain-containing protein"
                     /protein_id="HZN84099.1"
                     /translation="MTRMGDLLGPDPVLLPGDSAAEAELAASENPSIVAAAHPSASVA
                     WAALAEDALADDRAVTAYAYARTGYHRGLDQLRRNGWKGFGPVPYSHEPNRGFLRCVA
                     ALAKAADSIGETDEYQRCLDLLDDCDPAARSEFGL"
     gene            complement(7848..8741)
                     /locus_tag="VFC01_31095"
     CDS             complement(7848..8741)
                     /locus_tag="VFC01_31095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZN84100.1"
                     /translation="MGAGHDHSHADTRVSRMVIAAGILTAFFALELTTALLINSLALL
                     SDAGHMLTDLVAMFMGLTAVLLARHGSTSPKRTYGWHRAEVFTAVANAVLLLGVAAFI
                     LFEAVERLGNAPEIPGVPMIVVALAGLVANAVVVLLLRSHSKESLAVKGAYMEVVADT
                     VGSIGVLIAGIVTVTTGWPYADVVVAVFVAVWVLPRAIALARAALRILSESSPSHIDV
                     EELRSALCAVEGVTEVHDLHVWTLVPGKDMVTAHLTSTRNSASVLDDARAVLTARGLD
                     HATVQVEPPDDAADCKCEAEY"
     gene            complement(8872..9651)
                     /locus_tag="VFC01_31100"
     CDS             complement(8872..9651)
                     /locus_tag="VFC01_31100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZN84101.1"
                     /translation="MTIRPLHQSVRPSPIFLAIVALTVVGAVLAWLAESAAEPLAYVG
                     VFTFVIAGFLVSLCVHEFAHAYTAWRYGDRDVEARGYLTLNPLKYSHPLLSLGLPALF
                     IALGGIGLPGGAVYVRTSWMTKRQKTLVSLAGPATNLVFAIVLLVVTRLLWQQEHIVF
                     WGGMAFLGFLQVTAFVLNILPVPGLDGYSALEPHLSPQTQRALAPAKGWAFFVLLLLL
                     FTPQVNRWFFGVIFWLFDASGVPRGLIGWGMNLTRFWSAWF"
     gene            complement(9653..10582)
                     /locus_tag="VFC01_31105"
     CDS             complement(9653..10582)
                     /locus_tag="VFC01_31105"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN84102.1"
                     /translation="MSDIRVGIFAPVGQFAAPAEQRDAMLHRIADSGVDHVAVNDHVS
                     FFVGAGADALIDAATVLTRYPGVPCYVALYLLPLRHPVLVARQLASIAEHAPGRLVFG
                     VGIGGEDRHEFEICGVDPSTRGRRMDESLELLRALSDGKPVSFDGEFFKVRDAQILPA
                     PSSPIPITVGGRSDAAVRRAGRFGDGWLGIWVSPQRYGTVVEQVAEHAKTAGRNPSRF
                     QHALNVWCGFGATREAARAPLATGMQTFYQTPFEPFERYSPYGTPEDVAEFLYPYAKA
                     GCSVFNVIPCASDHETAVAGVAELRRLLRAVAS"
     gene            complement(10641..11228)
                     /locus_tag="VFC01_31110"
     CDS             complement(10641..11228)
                     /locus_tag="VFC01_31110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HZN84103.1"
                     /translation="MTTRDVLLVFGGRRVPRPLSDLPMVGLDDIADCRRIVVVGSDAD
                     LAAVLTHLLRADRLDVEVAHVRRPWHARRARTGQAQRIPLIRDETGTVIVGAAYWLPA
                     DEDARTIHGEAVTDDNLLFDGDVTGVRIEPTPTMPGLRASVLSSRMRPNRWLTGRAAQ
                     LGTDAALVVRDGVPAPRPVRRSTFYRHTEGWLRVR"
     gene            11310..12605
                     /locus_tag="VFC01_31115"
     CDS             11310..12605
                     /locus_tag="VFC01_31115"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZN84104.1"
                     /translation="MPAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVV
                     LPSGENFALHLIPSGILTPGVTNVIGNGVVVDPGVLLTELKGLEDRGVDTERLLISAD
                     AHLLMPYHVAIDKVVERYAGSKKIGTTGRGIGPCYQDKIARIGIRVADVLDEALLAQK
                     IEAALELKNQVLVKIYNRKALDAAEVVENLLAEAEGFKHRIADSRYLLNTALEKGETV
                     LLEGSQGTLLDVDHGTYPFVTSSNPTAGGAAVGSGIGPTRITTVLGILKAYTTRVGSG
                     PFPTELFDANGEYLSKTGGEFGVTTGRRRRCGWFDAVIARYATRVNGITDYFLTKLDV
                     LSSLETVPICVGYTVDGKRTDEMPMTQSDIGRAEPIYEELPGWWEDISNAREFDDLPA
                     KARDYVLRLEELAGAHVSCIGVGPGRDQTIVRRDILAAR"
     gene            12602..13237
                     /locus_tag="VFC01_31120"
     CDS             12602..13237
                     /locus_tag="VFC01_31120"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN84105.1"
                     /translation="MTDDPLLVDPDYDKHGGFPNFEAAEPGPGFARFLTAMRLAQDLA
                     VSADPDSDTWDLAADRAEELVKLLGPFEAAEGVGPANRVPSLPGAGSLLMPPYRVTRF
                     EPDGVELSVQFSRFSVGGNYAVHGGVLPLLFDSVFGMVIHAAGRPISRTAFLHVDYRK
                     VTPIDTPLTARGWVREAEGRKAFVNASLHDPEDNVLAEANGLMIRLLPGQP"
     gene            13280..13900
                     /locus_tag="VFC01_31125"
     CDS             13280..13900
                     /locus_tag="VFC01_31125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84106.1"
                     /translation="MSTPRAAAVAGVLFALLFATALVLIRLALPKGEEPGAQWLQTGS
                     TNLKIAATIMPFAGIAFLWFMGVVRDGLGRYEDKFFSTVFVGSGLLFLAMMFVAAGVG
                     AGLERSRAVGDPDVATFGQMVLLTVSKTYALRMAAVFMISLATIWLKTGLMPRWLVAV
                     SYLAAVGLLVASDITMWLVLAFPVWVLVVSLLLLARAGVIDIDRDD"
     gene            complement(13897..14088)
                     /locus_tag="VFC01_31130"
     CDS             complement(13897..14088)
                     /locus_tag="VFC01_31130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZN84107.1"
                     /translation="MKVVADRDVCISAGNCVMAASAVFDQDDDGIVVLLVDEVPEGEE
                     DHVREAVRLCPSQALRMAD"
     gene            complement(14085..15290)
                     /locus_tag="VFC01_31135"
     CDS             complement(14085..15290)
                     /locus_tag="VFC01_31135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN84108.1"
                     /translation="MTQAPELPPLHMRRVAFDPTPALSEIRETDGVRTMVNSFGMTVY
                     LVTRHEDIKDVLSDHERFSNGRPPGFVLPGATEIPEEEVTSARSGNLLSLDPPEHQRL
                     RRMLTAEFTIRRMKLLEPRIAEIVDAQLESMEKAGPPVDLVTNFALPIPSLVICELLG
                     VPYDDRDDFQQRSASQLDLSIPIPERLELQRQSRAYMRSLVERSRQQPGEDILGMLVR
                     EHGEELTDDELIGIASLLLLAGHETTSNMLGLGTLALLRHPDQLAAVRDNPDAIGPAV
                     EELLRWLSIVHTAIPRITTTDVEIAGVPIRAGELVFVSLPSGNRDPDFIDSPELLDIR
                     RGAPGHLAFGHGVHHCLGAPLARMEMRIAFPALLRRFPALALAEDFADVPFRSFHFIY
                     GLKSLEVTW"
     gene            complement(15300..16094)
                     /locus_tag="VFC01_31140"
     CDS             complement(15300..16094)
                     /locus_tag="VFC01_31140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN84109.1"
                     /translation="MIDTVLGSVRVQVRGTGEPMMFWPSLLMTGDMWAAQADHFGNEH
                     QVILVDPPGFGASEKLTAPFTFDQCARCIVDILDGLDLQRTHFVGNSWGGMIGGTFAA
                     TYPDRIGSAVLMNCTASPAGTRQKIEYGLLLRMAGLLGGIRPPLTRSVLKAFLGPTTF
                     TTRPDVVAFVRDTVQRVDLRSGGWAVRSVVPRRPDQRALLSKVTTPVLVVAGVEDATF
                     PLPETIAMAEAIPNAAIAVLEGVAHLAALENPSLVNKLIGEFVFRG"
     gene            complement(16102..>16296)
                     /locus_tag="VFC01_31145"
     CDS             complement(16102..>16296)
                     /locus_tag="VFC01_31145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HZN84110.1"
                     /translation="ERLGRDAEEIGESPAAAFRRLRLEMLAAERAAFIAERDSGQIDD
                     EVLREVLRGLDLEEATLNRG"
BASE COUNT         2788 a         5331 c         5538 g         2639 t
ORIGIN      
        1 cttgccgcac ccgcgcgcaa cgcggccacg gcacacatgc cgagagacaa agcatgccac
       61 ggtcacgggt gaccggtgtc gtgctggccg ccggcaactc gcgccgactg ggcacgccga
      121 aacagctgct gccatttgac gaaacaacgg tgctgggcgc gaccatagag gtcgcccggg
      181 gctgcccgtt cgaccagttg atcgtgacgc tcggcggtgc cgccaccgca gtgcgtgagg
      241 cggtgaatct cgagggcatc gacaccgtga tcgccgatga ccacgaatcg ggctgttctt
      301 cgtccctgcg ggccgctctg tcgcgggtgg atctgcgtgc cgaggggatc gtggtgatgt
      361 tgggcgatca gcccggcgta tcgcgcgaag cggtggccaa actcatcgat tcaggccgcg
      421 gtgccccgat cgctgtgtgt cgatactccg acgggatcgg ccatccgttc tggctgggcc
      481 gcccggtgtt cggcgaagtc gccgaactgc acggcgacaa gggcgtgtgg cgactgatcg
      541 agtcaggccg gttcgacgtg cggcaggtcc cgaccgacgg cacagttccg ctggacgttg
      601 acacctggga cgactaccag cggttgcttg ggtcggtggc acgatgaccg agcccgtcgc
      661 cggtctgttc tccgatgtcg acgaagtgat cagccggttc gatgcacacg actacctcct
      721 cgacgccggc acggcgtcgg caatctacct cgccgcagtg ctcggccgtc cgctgctgct
      781 ggaaggagaa cccggtgtcg gcaagaccac tgcggcgaaa acccttgcag cagttcttga
      841 tgcgccgttg cttcgattgc agtgctatga ggggttgacc gctgccgagg cactgtacga
      901 ctggaattac cagcgccagc tgctcagcat ccggctggcc gaggcgcgcg gcgaagccat
      961 cggcgagtcc gacctctaca ccgagacata cctggtggac cggccgattc tgcaatgcgt
     1021 gcgctaccgc gggcccggcg cgccggtcct gctgatcgac gagatcgacc gtgcggacga
     1081 cgaattcgag gcgctgctgc ttgagtttct cggcgaggcg gcagtgacgg ttcccgagct
     1141 gggcaccttt gtggcccagc gcccgccgat cgtcgtgctc acctcaaacc gaagccgtga
     1201 tctgcatgac gcactgcggc ggcgatgcct ctaccactgg atcgactatc ccgatccggt
     1261 gcgagcggca gcgatcgtgc gcaggacggt gccgggggcg acggcagcac tgattcagca
     1321 cgccgcccag ttcgtcggcg agacacgggt tctcgacatg gacaaaccgc ccgggatcgc
     1381 agagaccatc gactgggtgg cggccttggt tgcgctcggc gttgccgacc tggttgccgg
     1441 tgacgcgcgc acttcggcgg tcgtcagcct tggcgcgctg gccaagacgc ccgatgaccg
     1501 tggcgtcgtc gccgaggcat tcgctgacta catggaagga atctgccgat gaagatcacc
     1561 aacgagttca ctgtgagtgc cccggcagaa aaggcttggg aggtgctgac cgatctacag
     1621 caggtggttc cgctgatgcc gggtgcacag ctggtcggcc aggaaggtga tgactacctc
     1681 ggcaaggtca aggtgaaggt cggaccggtg accagcgagt tcagcggcaa ggcacacttc
     1741 gtggagcgag acggcaagac gcaccgcggc gtggtggacg cccgcggcaa ggaggcgcgc
     1801 gggaccggaa atgccgccgc caccatcact ttcggtgtgc acgaggccgg ggaccgcagc
     1861 agggtcacgg tcgacaccga cctcaagatc gtcggcaagc tcgcccagtt cggcggcggc
     1921 atgttgcagc aggtatcgga gaagctgctg ggcgagttcg tcgactcgtt ggaagccaag
     1981 cttgccgccg gcgatgccga agaagacgcg gcgcgcacgg caccgaccaa tgggcaggtc
     2041 gcaaggccgg cgccggagcc ggcgccgatc gacctgatcg agctggcggg cggcagtgcg
     2101 atcaagaatt acggcccggc ggttttcggc gctttcctgt tggcggctgt cgtcttcacg
     2161 ctgggtcggc tgcgaattgc gcgaaaggcg ctgcgcggga agcgatgacc gcgccgttcc
     2221 ttcttcgtgg cgtcgacctg gccgccttcg ccgttgcctt ggtcaaccgg ctgcactccg
     2281 gaggtgtggt ggtgtccgcc agcggcccaa cgggattcgt cgacgcgctg cgtcgtaact
     2341 ggccaagaac gcgctcgcag ctctactggg tggcgcggct gacgctggtg aaccgggtgg
     2401 aggacctggc cgctttcgat gcggtgttcg agtcggtgtt cgcggacgcc gtgctgggtg
     2461 tggatccacc gggcctgaag cagagcatgg gaacggccgc cgccgtccac cctggtgtgc
     2521 ccggccgcgc gagcaccctg gccgacggtg gcctgccgtg gaccacgcgc ccggcgtcgg
     2581 tcactgccgt cgacgcccgt accgccgaca tcgggatccc cgacgccctg ccgagccgga
     2641 tcgtcgcacg cgcggacgaa ccgtttgagc ggttcgatcc cgacgacctc cgtctgcttg
     2701 gcgaatggct ggagcaggcg gcggcccact ggccgcggcg acgcagccag cgccgagagt
     2761 tgcaccggca cggcaaacgg gtcgatctcc gggagacgat gaaggcgtcg cgcgcgaccg
     2821 gctgggagac gctgcgggtg gcgcgcaccc gcaaccgcga gtacccccgc cgggtggtcc
     2881 tcgtgtgcga cgtcagtcga tcgatgcaac cgtacgccac catctatctg catctcatgc
     2941 gagcagcggc cctgcggcag aatggaattc gccccgaggt gttcgcgttc tcgacgtcgc
     3001 tgacgcggct gaccgctgcg ttgtcgcatc gctcaccgga ggtggcgttg gccaaggcca
     3061 atgcgaaggt caccgaccgc tacggcggaa cgcatctggg ccgctgcgtc ggggaactcg
     3121 tttcggcatc ccacggcaac gcattgcgtg gcgcggtggt gatcatcgcg tccgacgggt
     3181 gggacagcga tccgcccgac gtgctcgaac gggcgctgat ccgggtgcgt cgccgggcgc
     3241 agaagctgat ttggctcaac ccacgctccg ctcagcccga cttccggccg ctggccgcgt
     3301 ccatggcggc ggcgttgccg tactgcgacg tgttcatgcc cgcgcactcg gtgacgggtc
     3361 ttcgcgagct gttcgctgtg cttgccgacc agtaggcggg cagctaggcc tcggctgcgg
     3421 gctccccggc ggttctcttg gcggcccggc gccgcttgta ccactcgatg aacatcggca
     3481 tcacggacac cagcgcgatg aggatgaaga tcggctcgag cagcttctgg atgatctcga
     3541 actggcccag cccgtacccg aggagtgtca ggccgacgcc ccagaccacc gcaccgatga
     3601 cattgaaaag cgtgaatgcg ccgtagccca tccgggcggc gcctgccgtg atcggcgcca
     3661 gggtccgcac gatcggcacg aagcgggcga gcacgatggc gaacgggcca cgctgctcga
     3721 agaagatgtg ggcctcgtcg aggtagcgct gcttgaggaa tcgcgcgttc ggcttgaaca
     3781 tcgcggtacc cagatagcgg ccgatgaagt atccgacctg tccgccgagg atggcggcga
     3841 tcgggatgaa gaccagtagc tgccacagct tgaagttggc ttgacctgcg gcggccgtcc
     3901 ccgcggcaag catgcccgcc acgaacagca gcgtgtcgcc tggtagcacg gggaacagca
     3961 cgcccgactc gacgaaaatc accaccagca gccccaccag cgcccaggta ccgaagtagc
     4021 cgatgagctt gatcggatcc aggaaatccg gcatgagcgc cagctgggtc gcagccgccg
     4081 agacggcggt ggtggtgtcc atggcccccc aagataccgg cgacgccctg aagggacgct
     4141 gtgccgggtt tatggtcggc tatggccacc actcatcgag ctgtgcacgt tgcgtccgcc
     4201 aatgcgcccc tgaccctcgt cgacgtcgag accacgccgc ccggccccgg ccatgtccgc
     4261 atcaccgtcg ccgcctgcgg ggtatgcggt accgatcacg gtttcgtcgc tggtggtttt
     4321 ccgaatatga cgtggccgtt gacgctggga cacgagctcg cgggaacgat cgcggaactg
     4381 ggcgacggtg tcgagggatt cgctgtcggc gaccgggtgg cggtcggctg gttcggcggc
     4441 aactgcaacc ggtgcatccc gtgccgccag ggcaagttca tgcaatgcga gaacggagag
     4501 gtgcccagct ggcactatcc cggcggctac gccgagtcgg tcacggcgcc ggccaccgcg
     4561 ctcgcccgaa ttcccgacgc actgtcgttc gtcgaagcgg caccgatggg ctgcgccggg
     4621 gtgacgacgt accacgcgct gcgcaagacc aaggcggtcg ccggggaccg ggtcgcggtg
     4681 ctgggcatcg gcgggctggg ccacctcggc gtgcagtggg ccagggcgat gggcttcgag
     4741 acgatcgcga tcgcccgcgg tgcggacaag gaagccaacg cccgggaact gggtgcccac
     4801 cactacatcg attcgagggc cgaggatgtc cccgccgcat tgcaggccct cggcggcgcg
     4861 tctgtcgtgc tcgcgacggc cgccaattcg gccgcgatgg cgcagaccgt cggcggcctg
     4921 gcgcccgagg gcgaactggt catcgtcggc gtcacggccg accccctgcc gatcgcgccc
     4981 ctccagctga tcagccctgg gttgtccgtg agcggtcacc cgtccggcac gtcgcgggat
     5041 atcgaggaga ccatgcattt cgccgtcctg tccggcgtgc gcgcgtggat cgaagagcgg
     5101 ccgctggccg aggcagccga ggcgtacgcg gcgatggacg agggccgtgc gcgctaccgc
     5161 atggtcctga cgatgtgagc ccgcctcgtt cttgagatac ttcaacaaac ctgtagcagc
     5221 cgggaggaac tgaaatgccc atcgcaacgc ccgaggtcta tgcggagatg ctgggtcgag
     5281 ccaaggagaa ctccttcgcg ttcccggcga tcaactgcgt cggatcggag agcatcaacg
     5341 ccgcgctcaa gggtttcgcc gacgcgggca gcgacggcat catccagttc tccaccggcg
     5401 gagccgaatt cggttcgggt ctcggcgtca aggacatggt gacgggcgcc gtcgcgctgg
     5461 ccgagttcgc ccacatcgtc gcggacaagt atccgatcac cgtcgcgctg cacacagacc
     5521 actgcccgaa ggacaagctg gacagctacg tcacgccgct gctggccatt tcgcagaagc
     5581 gggtcgccga gggccgcaac ccgctgttcc agtcgcacat gtgggacggg tcggcggtgc
     5641 ccatcgacga gaacctcgac atcgctcgcg agctgctcaa ggaggcctcg gccgccaaga
     5701 tcatcctcga gatcgagatc ggcgtggtcg gcggcgagga agacggcgtc gcccacgaga
     5761 tcaacgacaa gctctacacc acgcctgagg acttcgagaa gaccatcgac gcgctcggcg
     5821 caggcgagca cggaaggtac ctgctggccg cgacgttcgg caacgtgcac ggtgtgtaca
     5881 agccgggcaa cgtcaagctg cgccccgata tcctcgagca gggccagaag gtggccgcgg
     5941 ccaagctcgg attgcccgag ggcgccaagc ctttcgactt cgtgttccac ggcgggtccg
     6001 gctcgctgaa gtcggagatc gaggactcgc tgcgctacgg cgtggtgaag atgaatgtcg
     6061 acaccgacac ccagtacgcg ttcacccgcc cgctcgctgc gcatatgttc accaactacg
     6121 acggcgtgct gaaggtcgac ggcgaggtcg gtaacaaaaa ggcctacgac ccgcgaagtt
     6181 acctgaagaa ggccgaggcg agcatgtcgg agcgcgtcgt cgaggcgtgc aaagacctgc
     6241 aaagcgcggg tcgttcagtt tcgggaggct agggtgagca ctgtgaagcg tggaattctg
     6301 atcgcctgct gcacggtcgc gattcccatg ggaacgctcg ctgtcgccgc accggccggc
     6361 gccaacacct gcccctgggg caccgtgccc cgattcgacg gggtgtgcac gcagggacag
     6421 tccggcggga cgccgccacc cggcctcacc gttccgccgc agggcgccga cattgtcacc
     6481 atgccgggcc agttcccgac ggtcaacggc atcccgtgca cggacgagca cttcggcacc
     6541 tgctacgcga tgtcgcagaa cggctagctg tcgggggcct ggcagatctt ccactgatcg
     6601 tcgcggaact gcagatcgaa gctgcgcgtc gaacgcgtct gcggcgcata ggccatgaac
     6661 gtcgtgacat tggcctccgc gtggtcgccg ttgacgacca cctggtcgat gctcgcgacc
     6721 accggatact gcttcgctgc agcgacccgg gcgtgggtgt cggtccatgc ccggtcgtcg
     6781 tagttgacgt agttgtcgcg cgtggtgccg caggtgatgc ttcgtaacgt ggccaggtcg
     6841 ccgttctgga ccgcgacgtc gaagttctgg atcgtcgtgc ggactttgtc ctcttcggac
     6901 accttcggcg tcgagccgcg ggtcaacagc accgtgccga gaatcgcgac ggccaccaag
     6961 gcggcgatga ccaacagcag cgccaccacc cagccccagc tacgacggtt gcgcggcggc
     7021 tttggagggt cggtgcgtgg cggaataact tgcggcgcaa ccggtttcgc ttgtggcatg
     7081 gcctcggttg gcaccggcgg catcacctcg gtggcaggct cggccggcgt gtcgatcttc
     7141 tgtgtcgagc cgtcgaatcc tgagggcgcg gtgaaccgtc gctcagcagg cttgtccgtc
     7201 tcctcggccg gcagttgggc ctgagcctcg ggcgacaaca ggatctcggt ggcgggctcg
     7261 tgctccgggt cggtcacggc ttcagcgggc tgcgtctcat ccgtctcgac gacggcttcc
     7321 gccgctggct cggtggcttc gtccggatcg tccggccctg atgggttcga catccctgct
     7381 gcgcttcctc cctctacgag ctaccggtgc agcctaacgg cacaatgagg ccatgacgcg
     7441 catgggagat ctcctcggac cggatccggt actgctaccg ggcgacagcg cagcggaggc
     7501 cgaactcgcc gcctccgaga acccgtccat cgtcgccgcc gcgcatccgt cggcgtcggt
     7561 ggcgtgggcc gcactcgcgg aggacgcgct cgccgacgat agggccgtca ccgcctacgc
     7621 gtacgcccgc accggctatc accgcggcct ggatcagttg cgccgcaacg gatggaaggg
     7681 cttcggcccg gtgccctaca gccacgagcc caaccgcgga ttcctgcggt gtgtggcagc
     7741 gttggcgaag gccgccgact cgatcggcga gacggatgag tatcagcgct gcctggatct
     7801 gctcgacgac tgcgatcccg cggcgcggtc cgaattcggg ctgtaggtca gtactccgct
     7861 tcgcacttgc agtccgcggc gtcgtccggc ggctccacct gaaccgtcgc gtgatccagc
     7921 cctcgcgccg tcaacactgc gcgggcgtcg tcgagcaccg acgccgaatt ccgggtgctc
     7981 gtcaggtgcg cggtcaccat gtccttgccc ggcaccagcg tccacacgtg caggtcgtgc
     8041 acctcggtca cgccctcgac cgcacagagc gccgagcgca gctcctcgac gtcgatatgg
     8101 ctcggcgacg attcggacag aattcgcagc gcggcacggg ccagtgcgat cgcgcggggc
     8161 agcacccata ccgcgacgaa caccgcgacg accacgtcgg cgtaaggcca ccccgtcgtc
     8221 acggtgacga tgcccgcgat cagcacaccg atgctgccga cggtgtcggc gacgacctcc
     8281 atgtaggcgc ccttgacggc caggctctcc tttgagtgcg accgcagcag taacacgacg
     8341 acggcgttgg ccaccagccc tgccagtgcg accacgatca tcggcacacc ggggatctcg
     8401 ggtgcgttgc cgagtcgttc gaccgcctcg aagaggatga acgcggcgac accgagcagc
     8461 agcaccgcgt tcgcgacggc ggtgaacacc tcggcgcggt gccagccata ggtccgcttc
     8521 ggcgacgtgc tgccatgacg ggccaacagc accgccgtca ggcccatgaa catcgcgacc
     8581 aggtcggtga gcatgtggcc ggcgtcggag agcagggcaa gcgaattgat cagcagcgcc
     8641 gtggtgagct cgagggcgaa gaacgcggtc aggattcccg cagcgatgac catccgggaa
     8701 acacgggtat cggcatggct gtgatcgtgt ccggcaccca tgtctgcaat ctatgcgtaa
     8761 acacgcatat gtcgcaagtg gccccacgcc gcgcgagaag acgcaaaagt gtccaatttt
     8821 gggccttttc ggggatcttt gcgtcttctc gcgagagaaa agtggccgcc gtcagaacca
     8881 ggcggaccag aagcgggtca ggttcatgcc ccacccgatg agcccacggg gcacaccgga
     8941 ggcgtcgaat agccagaaga tgaccccgaa gaaccaccgg ttgacctgcg gggtgaacag
     9001 caggagcaac aggacgaaga acgcccaccc ttttgcgggg gccagcgcac gctgcgtctg
     9061 cgggctcagg tgcggctcca gcgcgctgta tccgtcaagt ccggggacgg gcaggatgtt
     9121 gagcacaaac gcggtcacct gcaggaagcc caggaacgcc ataccgcccc agaacactat
     9181 gtgctcctgt tgccagagca gacgagtcac cacgagcaac acgatcgcga acaccaggtt
     9241 ggtggccgga ccggccaggc tgacgagggt cttctgccgc ttcgtcatcc acgacgtgcg
     9301 cacgtacacc gcaccgcccg gcaggccgat accgcccagc gcgatgaaca gtgccggcag
     9361 cccgagcgac aacagcgggt gcgagtactt cagcgggttc agggtcagat agccgcgcgc
     9421 ctcgacgtcg cggtcgccgt agcgccacgc ggtgtaggcg tgggcgaact cgtgtacgca
     9481 aagcgacacc aggaagccgg cgatgacgaa cgtgaacacg ccgacgtacg ccagcggctc
     9541 agcagcgctt tcggccagcc aggcaagcac tgcgccgacg acggtgagcg cgacgatcgc
     9601 aaggaagatc gggctgggcc gcaccgattg gtgcagcgga cggatggtca cttcacgaag
     9661 ctaccgcgcg cagcagtcgt cgcagctccg caactccggc taccgcagtt tcatgatcac
     9721 tggcgcacgg aatcacgttg aacaccgagc agccggcctt cgcataggga tagaggaact
     9781 cggcgacgtc ctcgggtgtc ccgtacgggg aataacgctc gaagggttcg aacggcgtct
     9841 ggtagaaggt ctgcatacct gtggcgagtg gcgcgcgtgc ggcctcgcgg gtggcgccga
     9901 aaccacacca gacgttgaga gcgtgctgga agcgactcgg gttcctgccc gcggtcttag
     9961 cgtgctccgc gacctgctcc accacggtgc cgtaacgctg cggggaaacc cagatgccca
    10021 gccagccatc gccgaaccgg cctgcgcgcc gcaccgcggc gtcggaacgg ccgccgacgg
    10081 tgatcgggat cggcgacgac ggggccggaa ggatctgggc gtcgcgcacc ttgaagaatt
    10141 cgccgtcgaa gctgaccggc ttgccgtcgg acagcgcgcg caacagctcc agcgactcgt
    10201 ccattcggcg cccgcgggtg ctggggtcca caccgcagat ctcgaactcg tgcctatctt
    10261 cgccgccaat cccgacgccg aacaccagcc tgcccggtgc gtgctcggcg atgctggcaa
    10321 gctggcgtgc caccagcacc gggtggcgca gcgggagcag gtaaagcgcg acataacagg
    10381 ggaccccggg gtagcgggtg agcacggtcg cggcatcgat cagtgcatct gcgccagcgc
    10441 cgacgaaaaa gctgacgtgg tcgttgacgg ctacatggtc gacaccggaa tcggcgatgc
    10501 ggtgcagcat cgcgtcgcgc tgctcggcgg gagccgcgaa ctgcccgaca ggggcgaaga
    10561 tgcccactcg aatatcactc atgcggcaag cgtgaccccc caactttcag cagagcttgc
    10621 gaagcgctga aatcgcctgg tcagcgcacc cgcagccagc cctcggtgtg ccggtagaac
    10681 gtcgatcgcc gcaccggcct cggggcgggc acgccgtcgc gcaccaccag ggcggcgtca
    10741 gtgccgagct gtgcggcgcg gccggtcagc cagcggttgg gccgcatcct gctcgacagc
    10801 acgcttgccc gcagaccggg catcgtcggc gtcggctcga tccgcacccc ggtcacgtcg
    10861 ccgtcgaaca gcaggttgtc atcggtgacc gcttcgccgt ggatcgtgcg ggcatcttcg
    10921 tcggccggca gccagtacgc ggcgccgacg atgacggtcc ccgtctcgtc gcggatgagc
    10981 ggaatccgtt gcgcctgacc ggttcgcgcg cggcgcgcat gccagggtcg gcgaacgtgg
    11041 gcgacctcca cgtcgagccg gtccgctcgt aacaagtggg tcagcaccgc cgcgaggtcg
    11101 gcgtccgagc ccaccaccac gatcctgcgg cagtcggcga tgtcgtcgag gccgaccatc
    11161 ggcaggtcgc ttagcggccg gggcactcga cggccgccga aaaccaggag gacatcgcgg
    11221 gtggtcaacg cgaactcctc aggtcggctg ggatagggtg ggtcaccggc tgccacagtt
    11281 ttaaggcaga tagagcggga gactatgtca tgccggcaat cgtgctcatc ggtgcccaat
    11341 ggggggacga gggcaaagga aaggcgaccg acctgctcgg tggccgcgtc caatgggtgg
    11401 ttcgttacca aggcggcaac aacgccggcc acaccgtggt gttgccgagt ggcgagaact
    11461 tcgcccttca cctgattccg tcgggaatcc tcactccggg cgtcacgaac gtgatcggca
    11521 acggcgtcgt cgtcgatccc ggggtgttgc tcaccgagtt gaagggcctc gaggatcgcg
    11581 gcgtcgacac cgagcggctg ctgatctccg ccgacgcgca cctgttgatg ccgtatcacg
    11641 tggccatcga caaggtcgtg gaacgctacg cgggcagcaa gaagatcggc accaccggcc
    11701 gcggtatcgg cccgtgctac caggacaaga tcgcacgcat cgggatccgt gtcgccgacg
    11761 ttctcgacga ggcgctgctg gcccaaaaga tcgaggccgc actggaactc aagaaccagg
    11821 tgcttgtcaa gatctacaac cgcaaggcgc ttgacgccgc cgaggtggtc gagaacctgc
    11881 tggccgaagc cgagggattc aagcaccgca tcgccgacag ccgctacctg ctcaacaccg
    11941 cactcgaaaa gggtgagacg gtgctgctgg aaggttcgca gggcacactg ctcgacgtcg
    12001 accacggcac gtatcccttt gtcacgtcgt cgaatccgac cgcgggtggc gccgccgtcg
    12061 gttcgggcat cggcccgacg cgcatcacca cggtgctggg aatcctcaag gcgtacacca
    12121 cccgagtggg ctcgggcccg ttcccgaccg agttgttcga cgcgaacggc gagtacctgt
    12181 cgaagaccgg cggcgagttc ggggtgacga cgggacggcg tcgccgctgc ggatggttcg
    12241 acgcggtcat cgcccgctat gccactcgcg tcaacgggat caccgactac ttcctgacca
    12301 agcttgacgt gctgtccagc ctcgagacgg taccgatctg cgtcggctac accgtggacg
    12361 gcaagcgcac cgacgagatg ccgatgaccc agagcgatat cggccgcgcg gaaccgattt
    12421 acgaagagct gcccggctgg tgggaggaca tctccaacgc ccgcgagttc gacgatctgc
    12481 ctgccaaggc acgcgactac gtgttgcggc tggaagagct tgccggagca catgtttcgt
    12541 gcatcggcgt cgggcccggc cgggatcaga ccatcgtgcg ccgcgacatc ctggcggcac
    12601 ggtgacggac gatccacttc tcgtcgaccc cgattacgac aagcatggtg ggttcccgaa
    12661 cttcgaagca gccgagcccg gcccgggatt cgcacggttt ctgaccgcga tgcgactggc
    12721 gcaggatctt gcggtgtcgg cggaccctga cagcgacacg tgggatctgg ctgccgatcg
    12781 cgccgaggaa ctcgtgaagc tgctcggtcc gttcgaggcg gccgaaggcg tcgggcccgc
    12841 caaccgcgtc ccctcgctgc ccggcgcggg cagcctgctg atgccgccgt atcgggtgac
    12901 caggttcgaa cccgatggag tcgaattgag cgtgcagttc agccggttct cggtcggcgg
    12961 aaactacgcg gtgcacggcg gcgtgctgcc gctgctgttc gactcggtgt tcggcatggt
    13021 catccatgcg gcagggcggc cgatcagccg caccgccttc ctgcacgtcg actaccggaa
    13081 ggtgacgccg atcgacacgc cgctgaccgc gcgtggctgg gtgcgggagg ccgagggccg
    13141 caaggcgttc gtcaacgcat cgctgcatga tccggaggac aacgtgctcg cggaggcgaa
    13201 cgggttgatg atcagattgc tccccggcca gccgtagccg tgccacgatg acggcgtgac
    13261 tgaacgtcaa cccagccggt tgtcgacgcc gcgagcggcg gcggtggccg gtgtgctgtt
    13321 cgcgctgttg ttcgccaccg cgctggtcct catccggctg gcgctaccga agggcgaaga
    13381 gccgggcgcg cagtggctgc agaccggcag cacgaatctc aaaatcgcag cgacgatcat
    13441 gccgttcgcg ggcatcgcgt tcctgtggtt catgggggtg gtgcgcgacg ggctcgggcg
    13501 ctacgaggac aagttcttct cgacggtatt cgtgggcagc gggctgttgt tcctcgcgat
    13561 gatgttcgtt gcggccgggg tgggtgcggg tctggagcgc agccgtgcgg taggtgatcc
    13621 cgacgtggcg acgtttgggc agatggtgtt gttgactgtc tcgaagacct atgcgctgcg
    13681 gatggcggcg gtgttcatga tctcgctggc gaccatctgg ctgaagacgg gactgatgcc
    13741 gcggtggctg gtcgcggtga gttaccttgc cgcggtcgga cttctggtcg ctagcgacat
    13801 cacgatgtgg ctcgtactgg cgtttccggt gtgggtgttg gtggtcagcc tgctgctgtt
    13861 ggcgcgggct ggcgtcatcg acatcgaccg tgacgattag tctgccatcc gcaaagcctg
    13921 tgacggacaa agccttacgg cctcacgaac gtggtcctct tccccctcgg gcacctcgtc
    13981 gaccagcagc acgacgatcc cgtcgtcgtc ctgatcgaag acggcgctgg cggccatcac
    14041 gcagtttccc gcgctgatgc acacgtctcg gtcagccacg actttcacca tgtcacctcc
    14101 agcgatttca acccgtagat gaagtgaaat gaccggaagg gcacgtcggc gaagtcttcg
    14161 gccagcgcca gcgcggggaa acggcgcagc aacgccggga acgcgatccg catctccatg
    14221 cgtgccagcg gggcgcccag gcagtggtgc acaccgtggc cgaacgccag atgccctggc
    14281 gcgccccgac gaatgtcgag cagctcgggc gaatcgatga agtccgggtc gcgattgccc
    14341 gacggcagcg acacgaacac cagctctccg gcccgaatcg gcacgccggc gatctcgacg
    14401 tcggtggtgg tgatccgcgg aatcgcggtg tgcacgatcg acagccagcg cagcagctcc
    14461 tcgacggcgg gtccgatcgc atccgggttg tcgcgcaccg cggccagctg gtcgggatgg
    14521 cgcagcagtg ccagcgtgcc caggcccagc atgttcgacg tcgtctcgtg gccggcgagc
    14581 agcaataggc ttgcgatgcc gatgagttcg tcgtcggtca gctcttcgcc gtgttcgcgc
    14641 accagcatgc cgaggatgtc ctcgccgggc tgttggcgtg accgctcgac cagtgagcgc
    14701 atgtaggcac ggctctggcg ctgcaattcc agccgctcgg gaatgggaat ggacaggtcg
    14761 agctggctgg cgctgcgctg ctggaagtcg tcgcggtcgt cgtacggcac accgagcagc
    14821 tcgcagatca ccagtgacgg gatcggcaac gcaaagttcg taaccaaatc gactgggggt
    14881 ccggcctttt ccatcgattc caattgcgcg tcgacgatct cggcgatgcg cggctcgagc
    14941 agcttcatcc tccggatggt gaattcggcg gtcagcatgc gccgcaaccg ctgatgctcc
    15001 ggcgggtcga ggctcaatag attgcctgac cgtgcgctgg tcacttcctc ctcggggatc
    15061 tccgtcgcgc cgggcagaac gaaaccaggc gggcggccgt tggagaaccg ttcgtggtca
    15121 gacagcacgt ccttgatgtc ctcgtgccgg gtgaccaggt acaccgtcat gccgaacgaa
    15181 ttgaccatgg tgcgcacacc gtcggtctcg cggatctcgc tgagcgctgg ggtcggatcg
    15241 aacgcgaccc gccgcatatg cagcggcggc agctcgggtg cttgggtcat gaatcgacgc
    15301 tacccgcgaa acacaaactc cccgatcagc ttgttgacaa gcgaagggtt ctccagcgca
    15361 gccagatgcg ctaccccttc gagcacggcg atggccgcat tggggatcgc ctcggccatc
    15421 gcgatcgtct cgggcaacgg gaacgtcgca tcctcgacac cggcgaccac caacaccggc
    15481 gtcgtcacct tcgacagcag cgcgcgctgg tcgggacggc gcggcaccac gctgcgcacc
    15541 gcccagccgc cagagcgcag gtcgacgcgc tgcaccgtgt cgcggacgaa cgccacgaca
    15601 tcgggccgtg tcgtgaacgt cgtcgggccg aggaacgcct tgagcaccga tcgggtcagc
    15661 ggcggccgga tcccgccgag caggccggcc atccgcaaca ggaggccgta ctcgatcttc
    15721 tgccgtgtgc cggccggcga cgcggtgcag ttcatcaaca ccgcgctgcc gatgcgatcg
    15781 ggataggtgg ccgcgaaagt cccgccgatc atgccgcccc aagagttccc gacgaagtgc
    15841 gtacgctgca ggtcgaggcc gtcgaggatg tcgacgatgc agcgcgcaca ctgatcgaaa
    15901 gtaaaaggcg ccgtgagctt ttcgcttgcg ccgaagcccg gaggatcgac gaggatcacc
    15961 tggtgctcgt taccgaaatg atcggcctgc gcggcccaca tgtcgcccgt catcagcaag
    16021 ctgggccaga acatcatcgg ctcgccggtg ccccggacct gcacccgcac cgatcccagc
    16081 acggtgtcga tcattcgcga ttcagccacg gttcagtgtc gcctcttcga ggtcgagccc
    16141 gcgcaggact tcgcgcagca cttcgtcgtc gatctgaccg ctgtcgcgtt cggcgatgaa
    16201 cgccgcgcgc tcggccgcca gcatctccag ccgcaatcgc cggaacgccg ctgccgggct
    16261 ttcgccgatc tcctcggcat cgcggcccaa ccgctc
//
LOCUS       DASRJA010000693        26050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3476093,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000693 DASRJA010000000
VERSION     DASRJA010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 26050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26050
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3476093"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="VFC01_31150"
     CDS             complement(<1..1567)
                     /locus_tag="VFC01_31150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN84111.1"
                     /translation="MTATDTQPVDVPEDDWRGRVKDSQNDDLPIDESLPRRREARALL
                     FSLLRPYRVTVALLAIVVVVENVARLSVPLLVQRGIDHGIPPILSGGTAHTLMVVVGV
                     LCGVVLIQATSRMFFLQRSGRIGQKVLLELRRRVYRHFQRLDIAFHERYTSGRVVSRS
                     TNDVEAIQDMLETGFDSLITAVLTLIGTAILLVTLDVRLGLMCLAAFPVLVALVWWFR
                     DESAKTYRRVRESAALVIVQFVETMTGIKAVQAYRREPRNQEIFEDIADRYKADNEKT
                     FRLLAVFMPGVKLVGNITTGVVLLYGGYRVLHGQMTIGTLAAFLLYLRMFFEPMQEIS
                     QFFNTFQSASSALEKLAGVLAERPGIKEPTHPVALDKVRGDVAFHDVQFSYVKDRPVL
                     PDLSFSVPAGQTVALVGTTGAGKTTIAKLIARFYDPTSGAVTLDGIDLRDISQSELRR
                     HVVMVTQENFMFDGTVADNIRFGRPDATDEQVRSAAAAVGADRFIDALPEGYDTDVAK
                     RGGRLSAGQRQLVA"
     gene            complement(1564..3501)
                     /locus_tag="VFC01_31155"
     CDS             complement(1564..3501)
                     /locus_tag="VFC01_31155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN84112.1"
                     /translation="MSDTPTTLRLAPAIARPPARERTGSDLWRLMPYLLPYRARWIAM
                     VLIAVASLVATVAIPLMTKAVIDGPVRHQDQHGLWVLGAAAMGVGVSEAVLWFIRRWL
                     VARATMGVEADIRKDLYARLQILPMSFHGRWQSGQLLSRIMNDLSTIRRFMSFGMLFL
                     LLNTIQIAVVTAILLAMYWPLGVVVLASIVPITLTVLHFQQEYTKLSRLAQDQSGHVA
                     THVEESALGVRVVKSFGREDHVYDRFDKQLTNLYDTQVSRVSVSAKFWTLLEVIPNLT
                     LIVVLGFGAYAAGHGYVTMGTLVAFITMMLSLVWPIASLGFLLSMTQESFTAANRIAE
                     IFDAPREIADGPSHEAPRGGRLELVDVGFRFPDAAVDDWALRHVNATVEPGETLALVG
                     STGSGKSVLAALLSRLYDVTEGEILIDGQDIRELSLPALRQAVATAFEDPTLFSMSVA
                     ENLRLGRPDATDEQMAQAIEVAAAQFVYDLPFGLDTRIGEQGMSLSGGQRQRLSLARA
                     ILSAPKILVLDDTLSALDVHTEAVVEEALRRVLHHVTGIVVAHRASTVLLADKVALLQ
                     DGTITHIGTHAQLLATVPQYRYLLAADDELDDRAERKCAWEDDESRERLGKAYEEQEA
                     LKKEAAASDENRFVTSEAEGR"
     gene            complement(3550..5436)
                     /locus_tag="VFC01_31160"
     CDS             complement(3550..5436)
                     /locus_tag="VFC01_31160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN84113.1"
                     /translation="MSIETVARQTLYRQAHARGGDIRSLIDRGLLRRIWRFADRHHRR
                     LGGFVAVSVVSALLAVATPILAGKVVDAIVRGGPAATIVLLAAVIAAVAFAETVVSLV
                     TRWLSSNIGEGLILDLRTAVFDHVQRMLVAFFTRTRTGALVSRLGSDVIGAQRAFSDT
                     LSGVVSNIVTLTLTLAVMLSISWQITLLSLFLVPLFVVPARRIGRTMARLSREAAINN
                     ATMNTQMTERFSAPGATLVKLFGSPSHESREFEARADRVRDIGVRTAMLQSTFMNSLT
                     LMSALALALVYGLGGVLAIGGHLQAGAIVSLALLLTRLYAPLTALANAHVEIASALVS
                     FERVFEVLDLVPLIRERPDAVAVPDGPVSVEFDDVRFSYPSADKVSLASLEEVAVLDD
                     RGGDEVLHGISFTARHGQMVALVGSSGAGKSTTASLLARLYDVDSGAVRLNGVDVRDL
                     TFASLKDTVGMVTQDGHLFHESIRSNLQLAAPGATDDALWCALERARLADVVGEMPDG
                     LDTVVGERGYRLSGGQRQRLTIARLLLGQSRVVVLDEATASLDSASEAAVQHALDEAL
                     ADRTALVIAHRLSTVRAADMILVVEGGRIVERGTHDQLLAHGGRYAELYDTQFGAQAD
                     PVAA"
     gene            complement(5489..6037)
                     /locus_tag="VFC01_31165"
     CDS             complement(5489..6037)
                     /locus_tag="VFC01_31165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZN84114.1"
                     /translation="MIHTYLQLAGLFASLSLLSIGGGNAVLPEMHLQAVKGHHWMTDS
                     QFADIFSISQTAPGPSILIVTLVGYGAGLAVGGVPGAIVGGLIATAAMLLPAASLMYA
                     VTLFWQKAQKSKWRIAVEKGFAPLTVGLIMATSLVMSRAADHDWRAYALTAICTLIFI
                     RTKTNPLIVVFAAGVLGYFGVV"
     gene            complement(6034..6597)
                     /locus_tag="VFC01_31170"
     CDS             complement(6034..6597)
                     /locus_tag="VFC01_31170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZN84115.1"
                     /translation="MSEVEAGPGRVSLFELAWTFNHIALASFGGGLSAWSREVLVVEK
                     QWMGEEEFLSAMTMCRILPGANQVNMAVFAGSKMRGLPGAAAALFGLCLMPVALIMVA
                     GFFYFRFKEIPAVKGVLHGASAAAVALTLAMVIQTGKKCLTGVVPALLFAAAFFLNGL
                     LRWPLPVVLAILAPLSLIWAWPRNRAA"
     gene            complement(6590..7579)
                     /locus_tag="VFC01_31175"
     CDS             complement(6590..7579)
                     /locus_tag="VFC01_31175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZN84116.1"
                     /translation="MLPLSPRLIVEPDDGLDPVREFINSAQNSLLIKQFTFTEPSLIA
                     AVIERKNAGVDVRVMLNAARSGGDRANDETYQQFQDAGISVKWSSSKFYVTHEKSIVV
                     DEKVALVATFNLMEKYFTLTRDYGVITENPYHVAQIVEVFNADWDERDWDCSAYEGLL
                     WSNSNSRYHMAQFIDTATERLDIQHPKYVDAVILERLAAAADRGVKVHVLCGGKHGIS
                     EWDILDTFASLRTLKRFGVKVHKQKNLRVHAKLIIVDNAHALVGSMNIDRSAFDLRRE
                     LGIMTSDPAVVGRLKAVFDSDWELSHHYEAPDPLDPSQHHEDDFPHDHDLMHE"
     gene            7731..8441
                     /gene="prrA"
                     /locus_tag="VFC01_31180"
     CDS             7731..8441
                     /gene="prrA"
                     /locus_tag="VFC01_31180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PrrA"
                     /protein_id="HZN84117.1"
                     /translation="MACMDSGAASPRVLVVDDDPDVLASLERGLRLSGFDVATAVDGA
                     EALRSATETRPDAIVLDINMPVLDGVSVVTALRAMDNDVPVCVLSARSSVDDRVAGLE
                     AGADDYLVKPFVLAELVARVKALLRRRGATATFSSETITVGPLEVDIPGRRARVNGAD
                     VDLTKREFDLLAVLAEHKTAVLSRAQLLELVWGYDFAADTNVVDVFIGYLRRKLEAGG
                     APRLLHTVRGVGFVLRTQ"
     gene            8446..9792
                     /locus_tag="VFC01_31185"
     CDS             8446..9792
                     /locus_tag="VFC01_31185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZN84118.1"
                     /translation="MNVLSRFIRRTPSLRARVVVATAIGAAIPVLIVGAVVWVGITND
                     RQERLDRRLDEAAGFAIPFLPRGLDEIPKSPNDKDAVITIRRPDGQVTSNSDVVLPAL
                     SPGYEDTYVNGVRYRVRTVEIRGPQGPMAVAVGDTYDATIADTNNLHRRVIIICFFAV
                     GAAAVFAWVLAAFAVQPFKQLAQQTRQIDAGDEPPNIEVRGATEAVEIADAVKGMLQR
                     IWEAQDRTKAALASARDFSAVSAHELRTPLTAMRTNLEVLSTLDLPEEQRKEVVNDVI
                     RTQSRIEATLGALERLAQGELSTSDDHVPVDITELLDRAAHDAMRVYPDLDVSLVPAP
                     TVIIVGLPAGLRLAVDNAIANAVKHGGARRVQLSAVSSRAGVEIAIDDDGVGIPEEER
                     NVVFDRFSRGSTASHSGSGLGLALVAQQAELHGGTASLEDSPLGGARLLLRLPGPR"
     gene            9901..10197
                     /locus_tag="VFC01_31190"
     CDS             9901..10197
                     /locus_tag="VFC01_31190"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HZN84119.1"
                     /translation="MANYIALISWTEQGVKAYGNTLDRAEAAAELAGKLGGSLSQIFW
                     TMGEYDVVGVLEAPDDESATAFALALSSQGNVRTSTMRAFDAGEMRQILAKVPG"
     gene            complement(10232..10594)
                     /locus_tag="VFC01_31195"
     CDS             complement(10232..10594)
                     /locus_tag="VFC01_31195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZN84120.1"
                     /translation="MSTKPEIEFPDGPAPAELLINDLVVGDGAEAVPGGTVEVHYVGV
                     EYDTGEEFDSSWNRGESIEFPLRGLIQGWQDGIPGMKVGGRRQLTIPPEQAYGPAGGG
                     HRLSGKTLIFVIDLLATR"
     gene            10618..10866
                     /locus_tag="VFC01_31200"
     CDS             10618..10866
                     /locus_tag="VFC01_31200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HZN84121.1"
                     /translation="MPKDQDILAQVNKLVAEEQELRSKLQHHEIDESEEHQRLRAVEV
                     ELDQCWDLLRQRRALRETGGDPREARVRPPGEVEGYLG"
     gene            10870..12405
                     /locus_tag="VFC01_31205"
     CDS             10870..12405
                     /locus_tag="VFC01_31205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN84122.1"
                     /translation="MTEPDVVIVGGGHNGLVAAAYLARAGRRVQVLERLDHVGGAAVS
                     AHAFDGIDARLSRYSYLVSLLPRRIVDDLGARVRLVRRRYSSYTPDPATSGRTGLLIG
                     PQSTFGAVGAADDEAGFAEFYRRCRVVTSRLWPTLLQPLCTRDEARRHVGDDAAWRFV
                     VDEPIGRAITEAVSNDLVRGVMATDALIGTFARADDPTLMQNVCFLYHLLGGGTGDWD
                     VPVGGMGAVSGALAAAAAGYGAEILTGADVYAIDPDGEVRYRRDGQDHRVHADVVLAN
                     VTPVVLAKLLGEPEPTMAPGAQVKVNLMLRRLPRLRDGSVTPEQAFGGTFHINETFSQ
                     LDTAYTRADHGVVPDPLPCEIYCHSLTDPSILSDRLRDSGAQTLTVFGLHTPHSLVAG
                     KDPDRTRDSLTSAVLTSLNSVLAEPIQDVLMEDSAGRLCIEAKTTLDLEQTLGMTGGN
                     IFHGALSWPFADDDEPLETPAQRWGVATAHDRIMLCGSGSRRGGAVSGIGGHSAAMAV
                     LES"
     gene            complement(12543..12959)
                     /locus_tag="VFC01_31210"
     CDS             complement(12543..12959)
                     /locus_tag="VFC01_31210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN84123.1"
                     /translation="MSSNGLADEVWRAMASLVHDNRDGWKRAVVEASGLPFSRIRILK
                     RLSRQPMTVKQVARAATIDAPAATVAVNDLEDRGLVVRETDPTNRRCKVVSLTDAGRE
                     MVRKIDKVDDPAPTALAALDDTQLKELKAIIDLLGK"
     gene            complement(12949..14430)
                     /locus_tag="VFC01_31215"
     CDS             complement(12949..14430)
                     /locus_tag="VFC01_31215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZN84124.1"
                     /translation="MNETVVRSSPASRGPQSARRKGIILVSCCLSLLIVSMDATIVNV
                     AIPNIRADLHATASQLQWVIDIYTLVLASLLLLSGATADRFGRRRTFQIGLTVFALGS
                     LLCSLSPNVQILILARMLQAIGGSMLNPVAMSIITQVFTGRVERARAIGVWGGVVGIS
                     MALGPIVGGALIELVNWRAVFWINLPICAAAIVLTAIFVPESRSVTMRDVDPVGQGLG
                     MTFLFGTVYVLIEGPARGWTDARNMVVAIVALLAFVAFLRYESRRRDPFIDLRFFRSI
                     PFASATMIAVCAFAAWGAFLFVMSLYLQSERGFSAMHTGLIYLPVAVGALIFSPLSGR
                     MVGRFGARPSLLIAGTLITAATLLLTQLTVTTAVWQLLAIFAVFGIGFSMVNAPITNA
                     AVSGMPTDRAGAASAVASTSRQVGVSVGVALCGSVVGSALATTGVDFAVAARPLWLIC
                     AGLGLVIIVLAVYSTSARAARSAERLAPLIAGPDVRYEAADVK"
     gene            complement(14498..15805)
                     /locus_tag="VFC01_31220"
     CDS             complement(14498..15805)
                     /locus_tag="VFC01_31220"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HZN84125.1"
                     /translation="MADNPSSGEEHATLSYPGGELDLDIVGATEGADGIALGSLLSKS
                     GYTTFDEGFVNTASTKSAITYIDGDAGILRYRGYPIEQLAEKSNFIEVSYLLIYGELP
                     DKDQLEKFTTQIQRHTLLHEDLKRFFDGFPRNAHPMPVLSSAVNALSAYYQDSLDPFD
                     DAQVELSTIRLLAKLPTIAAYAYKKSVGQPFLYPDNSLTLVENFLRMTFGFPAEPYEV
                     DPELVRALDMLFILHADHEQNCSTSTVRLVGSSQANLFTSISGGINALWGPLHGGANQ
                     AVLEMLDKIRQSDGDTHDFVKKVKNREDGVKLMGFGHRVYKNYDPRARIVKEQADKIL
                     GKLGGDDELLDIAKALEEVALTDDYFVERKLYPNVDFYTGVIYRAMGFPTRMFTVLFA
                     LGRLPGWIAHWREMHDEGSGKIGRPRQVYTGYTERDYKALDGR"
     gene            complement(15901..16548)
                     /locus_tag="VFC01_31225"
     CDS             complement(15901..16548)
                     /locus_tag="VFC01_31225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN84126.1"
                     /translation="MAEVRGEPALGLRERKKQRTRATLIDAAVELCERQGFERTTVDQ
                     IAAIADVSPRTFSRYFPTKDAIALALIDEALELTAAELARQPHDISHFEALRRSYVAM
                     YNGTKTTPPGGLSVDRMMCTVRIVLSSAALRQAAVEFRPHPVTIELAKRMGVELYDRR
                     LRLVAAVWASIIMTALSDLGEGTDWTQVGIDDIITRLEATFAEFMGEIENARHPV"
     gene            complement(16618..17895)
                     /locus_tag="VFC01_31230"
     CDS             complement(16618..17895)
                     /locus_tag="VFC01_31230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN84127.1"
                     /translation="MRKFFADTTPLRTPDFRRLWLTGIVTVIGGNLTIFAVPVQLYAL
                     TQNSAYVGLSGLFALVPLIVFGLLGGAWADAMDRRTLLIIASIGLAVSSVMLWLQAAL
                     ALNNVWVVLCLLSVQQAFYAINSPTRSAAIPRMIPGDQLAAANSLNFTVFQFGAIVGP
                     LLAGVMLHWVDLSTLYLIDAITCIAPIWATFRLAPMPPTNGGNGPSGYGFTAVLDGFR
                     YLAGNRVVLMSFVVDLIAMILGMPRALFPQMAHESFGGPVEGGTTMALLAAAMSAGAV
                     AGGVFSGWFPRIQRQGLAVVVSIVVWGGAMIGFGLAGGLAHGHTGALLWIALAFLAIG
                     GAADMVSAAFRSTILQQAASDDLRGRLQGVFTVVVAGGPRLADAVHGAAAAVVGTTVA
                     AAGGGVLVVVGVVIAALTVPAFIRYRVAEGDKV"
     gene            complement(17895..18557)
                     /gene="pdxH"
                     /locus_tag="VFC01_31235"
     CDS             complement(17895..18557)
                     /gene="pdxH"
                     /locus_tag="VFC01_31235"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HZN84128.1"
                     /translation="MATRDDEHLVRMRVEYGSVEKDGSPDLDADWLADGWVALLRKWL
                     ADAEGAGIAEPNAMVVGTVDERGRPVTRAVLCKSVDETGISFYTNYDSAKGEQLAANA
                     YASATFPWFLLGRQVHVRGPVTKVSAEATAAYWANRPRGSQLGAWASHQSKPIASRAA
                     LLEQLADVTERFADHDEVPVPPNWGGYLIAPEVVEFWQGRENRVHNRIRLSEGRIERL
                     QP"
     gene            18641..19759
                     /locus_tag="VFC01_31240"
     CDS             18641..19759
                     /locus_tag="VFC01_31240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase 2"
                     /protein_id="HZN84129.1"
                     /translation="MTAVPEDFVEGLEGVVAFTTEIAEPDKDGGALRYRGVDIEDLVN
                     QQVTFGDVWALLVDGKFGHSLPPAEPFPLPIHSGDVRVDVQAGLAMLAPIWGYPPLLD
                     IEDETAREQLARASVMALSYVAQSARGIYQPAVPQRRIDECSTVTERFMTRWQGDPDP
                     KHVEAIDAYWVSAAEHGMNASTFTARVIASTGADVAAALSGAIGAMSGPLHGGAPARV
                     IPMIEEAEKTGDARAVVAGILDRHEKLMGFGHRVYRAEDPRARVLRATAKRLEAPRYE
                     VAAALEQAALAELRERRPDRAIETNVEFWAAVILDFAQVPTKMMPAMFTCGRTAGWCA
                     HILEQKRLGKLVRPSAIYVGPGPRSPEAVEGWDQVSKS"
     gene            19756..20370
                     /locus_tag="VFC01_31245"
     CDS             19756..20370
                     /locus_tag="VFC01_31245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HZN84130.1"
                     /translation="MTDSVATFASAARSFAALVHEIPASAWDGPGLGEWDLRSLVGHA
                     SRSLITVSTYLQQPADREDITTPQEYYVRVNPATLGLAPAGVVERGRQAGRDLGDDPA
                     ARVDELLSQVLGELSGVDDPLITVIGGAGIRLQTYLPTRVFELAVHGVDIARAVGISY
                     ALPTDVLEEATVLAARIAGAEGHGEAVLLALTGRDGLPPSFSIV"
     gene            20411..20860
                     /locus_tag="VFC01_31250"
     CDS             20411..20860
                     /locus_tag="VFC01_31250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN84131.1"
                     /translation="MAIEVQPSVSPHLTVGDGAAAIDFYVKAFGAVELGRVPGPDGKL
                     MHAALQINGSTVLLNDDFPEYNDGKSSTPTALGGTPVTIHLTVTDVDSKFQQAVDAGA
                     TVVSALEDQFWGDRYGVLRDPFGHHWSLGQPVREVSPEEIAEAMKQH"
     gene            complement(20927..23554)
                     /locus_tag="VFC01_31255"
     CDS             complement(20927..23554)
                     /locus_tag="VFC01_31255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HZN84132.1"
                     /translation="MRAAAYVGRVGGLAVALGVGVAVVTGYGLPVAWADTPSSEPSST
                     ESSSTDTSTSTEASAPAESAEKPTATEPTQPSSTEPTSSTGSTTTQAPPGVVVSTGGA
                     QSASKSKDDEETTEPTKKPDSGKAADVTAESTKTSAPDSKVEAVEVKVDTDPEPELKS
                     GAEARIAVSSFAVQTMSSTADTESAAVTTALTPPTVPSLRPWPTAFDPGTLVTYVGGL
                     VSSVVSAFLSPFAAGAPTPPADPPSLWTLLAWVRRELFNGSPTINPVINPQTNGLITG
                     NLGAVDPDDDPLTYTVIGRPHNGGTLEIDDDGNFIYRPMNAMAAVGGTDSFTVVVSDE
                     TAGLHVHGPLGLLKFVPILGNFINPGGGDAVAQTITVNVDPVAGVNLSFPPTFHWGVA
                     HAGFQAEGGPGSPVDPNSDWYKWVHDPINQLLGISNGVPENGPGTYVKYDSDAALAET
                     ELGMNTFRMSIEWSRIFPNSTASVDISDEGGVITEDDLEALDELANQAEVQHYSDVFD
                     SLIAHHLEPLITVNHFTLPAWIHDPITTRPLAQLGLPAPDAGWLSPDTPVEFEKYAAY
                     VAWKYKDQVDNWTVLNEPVPPVLTEFLAIPSLVPNWPPGLIRPDLASTFLVNEAKGYV
                     AAYDAIHAWDDTAFVGFANNMIPARPANPVNPLDVQAADAWNNFYNRWFPNAVVNGWV
                     DANLDGIQTPDELHPEMAQKVDFIGVQYYGSQPMQGFGLAPIPGFPWLKGLPLRCAAS
                     EPTCSDFNQPTDPGGFREVLEIAASYGKPLWVTENGIADAGDSKRPPYIVSHIGVVQD
                     LVTHGMDIRGYTYWSFVDNLEWVEGYDLEFGLYGSDPTTPELERTPKQASIDAISGIA
                     ETNSLPLSVLAMYIPGLTV"
     gene            complement(23613..25274)
                     /locus_tag="VFC01_31260"
     CDS             complement(23613..25274)
                     /locus_tag="VFC01_31260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN84133.1"
                     /translation="MPHVITQSCCSDGSCVYACPVNCIHPSPDEPGFATAEMLYIDPA
                     ACVDCGACVSACPVGAIAPDTKLKPKQLPFIELNAAFYPKRESKLPPTSKLAPVIEAP
                     FVEARAGGPLTVAIVGSGPAAMYAADELLTQQGVRVNVFERLPTPYGLVRAGVAPDHQ
                     STKRVTKLFDRIARRRGFRFYLNVEVGSDLTHHELLEHHHAVLYAVGAPNDRRLDIEG
                     IGLPGTGTATEVVAWINGHPEFTDLPVDLSHERVVIIGNGNVALDVARILTTDPDALA
                     RTDISDHALAALRTSRVQEVVIAARRGPADSAFTLPELIGLTSTCEVVLDAADHDLVL
                     RDLAEESDPLTRNKLEILSKLGDASAPVTRPRIRLAYQLTPKRVVGDRCADGVEFTVT
                     GTDEVHLVSAGLVLTSIGYRGKPIRGLPFDESAAVVPNDGGRVIGAPGSYVAGWIKRG
                     PTGFIGTNKSCAAQTVHNLVADYNHGLLADPIGKPAALDKLARTRQPAVVDAEGWKAI
                     DAAEIARGGEDRPRDKFTTFADMLAAAAAAPTPRVHQRLLAGLLR"
     gene            complement(25282..>26050)
                     /locus_tag="VFC01_31265"
     CDS             complement(25282..>26050)
                     /locus_tag="VFC01_31265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HZN84134.1"
                     /translation="EIGMWRQANVAKVGLHFESILIRGLMDYSFWTPNGSPEYRYCLH
                     EAVEECNHTMMFQEMVNRIGADVPGMPRMLKWVQPLIPLVAGPLPIPFWFGILAGEEP
                     IDHTQKNVLREGKSLHPIMERVMAIHVAEEARHISFAHEYLRKRIPHLPRRKRFWLSI
                     YVPLVMRILCSAIIVPPRAFWKEFDIPRSVRKEVFFRSPESRQMLRDMFGDVRMLCKD
                     TGLINPIAKLVWRICKIDGRPSRYRSEPQRQHVVAAA"
BASE COUNT         4599 a         8572 c         8536 g         4343 t
ORIGIN      
        1 acgccaccag ctgccgctgc cccgccgaaa gccggccgcc gcgcttggcg acgtcggtgt
       61 cgtaaccctc gggcagtgca tcgatgaatc ggtccgcgcc gacggcggcc gccgctgagc
      121 gcacctgctc atcagtcgcg tcgggcctgc cgaaccggat gttgtcggcg actgtgccgt
      181 cgaacatgaa gttctcctgc gtgaccatca cgacgtggcg tcgcagctcg ctctgcgaaa
      241 tgtccctcag gtcaatgcca tccagcgtca cggcacccga cgtcgggtcg tagaaccggg
      301 cgatcagttt ggcgatcgtg gtcttgcccg caccggtggt gcccaccagt gcgacggtct
      361 gacccgccgg aaccgagaag ctcaggtcgg gcagcaccgg tcggtccttc acgtaggaga
      421 actgcacgtc gtggaacgca acgtcgccgc gcaccttatc gagggcgacg gggtgcgtcg
      481 gctccttgat gcccggccgc tctgcgagca cgcctgccag cttctccagc gccgacgacg
      541 cggactggaa cgtgttgaag aactgcgaaa tctcctgcat cggttcgaag aacatccgca
      601 gatacagcag gaacgccgcc agcgtgccga tggtcatctg gccatgcagc acccggtagc
      661 caccgtagag cagcacgacg ccggtggtga tgttgccgac cagcttcaca ccgggcatga
      721 acacagccag cagccggaac gtcttctcgt tgtccgcctt gtagcgatcg gcgatgtctt
      781 cgaagatctc ctgattgcgc ggctcgcgac ggtaggcctg caccgccttg atgccggtca
      841 tcgtttcgac gaactgcacg atcaccagcg cggcgctctc ccgcaccctg cggtaggtct
      901 tggccgactc gtcgcggaac caccacacca gcgccaccag aaccgggaac gccgccaggc
      961 acatcaggcc cagccgaacg tcaagtgtca caaggagaat ggcggtgccg atcagcgtga
     1021 gcacggcggt gatcaggctg tcgaaaccgg tctccagcat gtcttggatg gcctcgacgt
     1081 cgttggtgga ccggctcaca actcggcccg acgtgtaccg ctcgtggaat gcgatgtcga
     1141 ggcgctggaa gtggcggtac acccgccgcc gcagttcgag cagcaccttc tgcccgatcc
     1201 ggccggagcg ctgcaggaag aacatccggc tggtggcctg aatcagaacc actccgcata
     1261 gcacgccgac gaccaccatc agcgtgtgcg ctgtaccgcc ggacaggatc ggcgggatgc
     1321 cgtggtcgat gccgcgctgt acgagtagcg gaactgacag gcgcgcaacg ttttccacca
     1381 cgacgacgat cgcgagcagc gcgaccgtca cacggtacgg ccgcagcagc gagaacagca
     1441 gcgcccgagc ctcacggcgc cgcggcaggc tttcgtcgat gggcaggtcg tcgttctggc
     1501 tgtccttgac ccggccgcgc cagtcgtcct cggggacgtc gactggttgg gtgtctgtcg
     1561 cggtcatcgg ccctcggcct ccgatgtcac gaaacggttt tcatcggaag cggccgcctc
     1621 ctttttcaag gcctcctgct cctcgtacgc cttgcccagc cgttcgcggc tctcgtcgtc
     1681 ctcccatgcg cacttgcgtt cggcgcgatc gtcgagttca tcgtcggcgg ccagcaggta
     1741 gcggtactgc ggcacggtgg ccaacagttg ggcgtgcgtg ccgatgtgcg tgatagtgcc
     1801 gtcttgcagc agcgccacct tgtcggccag caggaccgtc gacgcccgat gcgcaacgac
     1861 gatgccggtg acgtggtgca gcacccggcg caaggcctcc tcgacgacgg cttcggtgtg
     1921 cacgtcgagc gcggacagcg tgtcgtcgag cacgaggatc ttgggcgcgg agagaatggc
     1981 ccgcgccagc gacaaccgct gccgctgccc gccggacaga ctcatgccct gctcgccgat
     2041 gcgggtgtcc aacccgaacg gcaggtcgta gacgaactgc gcggcggcga cctcgatggc
     2101 ctgcgccatc tgctcgtcgg tagcgtccgg acgccccagt cgcaggttct cagcgaccga
     2161 catcgagaac agcgtcgggt cctcgaacgc ggtggccacc gcctgccgca gcgcaggcag
     2221 gctgagctcg cggatgtctt ggccgtcgat gaggatctcg ccctcggtga cgtcgtacag
     2281 ccgcgacagc aacgcggcca gcaccgactt gcccgaaccc gtcgagccga ccaacgccag
     2341 cgtctccccc ggctcgacgg tggcgttgac gtgccgcagc gcccagtcgt ctactgcggc
     2401 atcggggaaa cggaagccga cgtcgaccag ctcgagccgc ccgccgcgcg gcgcctcatg
     2461 cgacggcccg tcggcgattt cccgtggggc gtcgaagatc tcggcgatcc ggttggcggc
     2521 ggtgaacgac tcctgcgtca tcgacagcag aaagcccagc gatgcgatcg gccacaccag
     2581 tgacagcatc atcgtgatga acgcgacgag cgtgcccatc gtgacgtagc catggcctgc
     2641 ggcgtacgcg ccgaagccga gcaccacgat cagcgtgagg ttgggaatga cctccagcag
     2701 ggtccagaac ttcgccgaca ccgacaccct gctgacctgc gtgtcgtaga ggttggtcag
     2761 ctgcttgtcg aaccggtcgt agacgtgatc ctcgcggccg aacgacttca ccactcgcac
     2821 accgagagca gattcctcga catgcgttgc gacgtggccg gactggtcct gcgccagccg
     2881 cgacaacttg gtgtactcct gctggaagtg cagcaccgtc aacgtgattg gcacgatcga
     2941 ggccaacacg accacaccga gcggccagta catcgcaagc aggatcgcgg tcacgacggc
     3001 gatctggatg gtgttgagca gcagaaacag catgccgaat gacatgaagc ggcgaatggt
     3061 gctcaggtcg ttcatgattc gcgacagcag ctggccggac tgccagcggc cgtggaacga
     3121 catcggcagg atctgcagcc gcgcgtagag gtccttgcgg atgtcggcct cgacgcccat
     3181 ggtggcgcgg gcgaccagcc agcggcggat gaaccacagc accgcctcgg agacgccgac
     3241 gcccatcgcg gctgcgccga gcacccacag gccgtgctga tcctgatgtc gcaccggccc
     3301 gtcgattacg gccttggtca tcagcggaat ggccacggtg gcaacgaggc tggcgaccgc
     3361 gatgagaacc atcgcgatcc accgcgcccg gtacggcagc aggtagggca tcagccgcca
     3421 gagatccgaa ccggtacgtt cgcgggccgg cggtcgcgcg atcgccggcg ctaagcgaag
     3481 cgtggttggg gtgtcagaca cgagacctca attgtcccat ggctcgcaaa cggttaacgc
     3541 gcgcgcagtt cacgctgcaa cagggtccgc ttgcgcgccg aactgggtgt cgtacagctc
     3601 ggcgtagcgc ccgccgtgcg ccagaagctg atcgtgcgta ccgcgttcga cgatgcggcc
     3661 gccctcgacg accaggatca tgtctgcagc gcgcaccgtg gacagccggt gggcgatcac
     3721 gagagcggtc cggtcggcca gcgcttcgtc cagcgcgtgc tgcacggcag cctcggatgc
     3781 ggagtccagc gacgccgtcg cctcatcaag caccaccacc cgcgactggc ccagcaggag
     3841 gcgggcaatc gtcagccgct gccgctgccc gccggacaaa cggtatccgc gctcaccgac
     3901 aaccgtgtcc agcccgtcgg gcatctcgcc gactacatcg gccagccgcg cacgttccag
     3961 cgcacaccag agcgcgtcgt cggtggcgcc gggcgcggcc agctgcaggt tggaccggat
     4021 cgattcatgg aacagatgcc cgtcctgagt gaccatgccg acggtgtcct tcagcgacgc
     4081 gaacgtcagg tcgcggacat cgacgccatt gaggcgcacc gcgcccgagt cgacgtcata
     4141 gagccgggcc agcagcgagg cggtcgtcga cttgcccgct ccggaggagc cgacgagtgc
     4201 caccatctgg ccgtgccgcg cggtaaatga aatgccgtgc agcacttcgt ctccgccacg
     4261 atcgtcgagc accgcgacct cttcgagcga ggccagcgac accttgtcag ccgacggata
     4321 ggagaaccgg acgtcgtcga actcgaccga aacaggcccg tcaggaacgg ccaccgcatc
     4381 gggccgctcc cggatcagcg gcaccaggtc gagcacctcg aagacccgct cgaagctgac
     4441 cagcgcgctg gcgatctcga catgagcgtt ggccagtgcg gtcaacggcg cgtacagccg
     4501 ggtcagcagc agcgccagcg acacgatcgc gcctgcctga aggtggccgc cgatggcgag
     4561 aacgccgccg aggccgtaca ccaacgccag cgccaacgcc gacatcaacg tcagcgagtt
     4621 catgaacgtg gactgcagca tggccgtgcg gacgccgata tcgcggactc ggtcggcgcg
     4681 cgcctcgaat tcacgcgact cgtgcgacgg gctgccgaac agcttgacca atgtggcgcc
     4741 gggcgccgaa aaccgttcgg tcatctgggt gttcatggtc gcgttgttga tcgccgcctc
     4801 ccgcgacagc cgcgccatcg tgcgcccgat gcgtcgcgca ggcacgacga acagcggcac
     4861 taggaacagc gacaacagcg tgatctgcca cgagatggag agcatgaccg ccagcgtcaa
     4921 cgtcagcgtg acgatgttgg acacgacacc ggacaacgtg tcggagaacg cgcgctgtgc
     4981 gccgatcacg tcgctgccga gccggctgac caacgcgccg gtgcgggtgc gcgtgaagaa
     5041 cgcgaccagc atgcgctgca cgtggtcgaa caccgcggtg cgcaggtcca agatcaggcc
     5101 ctcgccgatg ttggacgaca gccaccgcgt gacgagcgag acgacggtct ccgcgaacgc
     5161 gaccgcggca atgacggccg ccaaaagcac gatcgtcgcc gccggtcccc ctcgaacgat
     5221 ggcgtcgacg accttacccg ccagtatcgg ggtcgcgacg gccagcagtg cgctcacaac
     5281 actgaccgcg acgaagccgc ccagcctgcg gtgatgccga tcggcgaagc gccagatgcg
     5341 gcgcagtaat ccgcggtcga tgagcgagcg aatatcgcca ccgcgcgcat gcgcctgccg
     5401 gtacagggtt tgccgggcaa ccgtttcgat actcatgaaa tcaccgtaga acctcaacaa
     5461 tggatgaggt caacgacttc ccgcggcctc agaccacgcc gaaatacccc agtacgcccg
     5521 cggcgaaaac cacgatcagc gggttcgtct tggtgcggat gaagatcaac gtgcagatcg
     5581 cggtcagcgc gtaggcccgc cagtcgtggt cggcggcccg gctcatcacc aacgaggtgg
     5641 ccatgatcag gccgaccgtc agcggcgcga agcccttctc gacggcgatt cgccacttcg
     5701 acttctgcgc cttctgccag aacagcgtga ccgcgtacat caacgacgcc gccggaagca
     5761 gcatcgcggc ggtggcgatc agtcctccga cgatggctcc tgggacgccg ccgactgcga
     5821 gccccgcgcc gtagccgacc agcgtcacga tcaggatgct cggaccgggt gcggtttggg
     5881 atatcgagaa gatgtcggcg aactggctgt cggtcatcca gtgatgaccc ttgacggcct
     5941 gcagatgcat ctcgggcagt accgcgtttc cgccgccgat cgacagcagc gacagcgatg
     6001 cgaacaggcc ggcgagttga agataggtgt ggatcatgcg gcccggtttc tcggccacgc
     6061 ccagatcaga ctcagcggcg cgagaatggc caacaccacc ggaagtggcc accgcagaag
     6121 gccattcagg aagaacgccg ccgcgaacaa caacgccggc actacgcccg tgaggcattt
     6181 cttcccggtc tggatgacca ttgccaacgt cagcgcaaca gcggcggccg aggcgccgtg
     6241 cagcacgccc ttcacggcgg gtatctcttt gaaccggaag tagaagaatc ccgccaccat
     6301 gatcaaggcg accggcatca ggcacagccc gaagagcgcc gccgccgccc cgggcaggcc
     6361 acgcatcttc gacccggcga agaccgccat gttgacctga ttggcgcccg gcaggatccg
     6421 gcacatcgtc atcgccgaca ggaactcttc ctcacccatc cactgcttct cgacgaccag
     6481 cacctcgcgc gaccaggcgg acagcccgcc gccgaacgag gcaagcgcga tgtggttgaa
     6541 cgtccacgcg agttcgaaca gggagacccg accgggcccg gcctcgacct cactcatgca
     6601 tgaggtcgtg gtcgtgcggg aagtcgtctt cgtggtgctg ggacggatcc agcggatcgg
     6661 gcgcttcgta atggtgcgaa agctcccagt cgctgtcgaa gaccgctttc agccgcccca
     6721 cgactgccgg gtcgctagtc atgatgccga gttcgcggcg caagtcgaat gcgcttctgt
     6781 cgatgttcat cgagccgacc aatgcgtgcg cattgtcgac gatgatcaac ttcgcgtgca
     6841 cccgcaggtt cttctgtttg tgcaccttga cgccgaaccg tttcaacgtc cgcagtgatg
     6901 cgaaggtatc gagaatgtcc cattcgctga tgccgtgctt gccgccgcag agaacgtgga
     6961 ctttgacacc gcggtcggcg gcggccgcga ggcgttcaag aatgactgcg tccacgtatt
     7021 tcggatgctg gatatcgagc cgttccgtgg cggtgtcgat gaattgcgcc atgtggtagc
     7081 gcgaattgga attgctccac agcaggcctt cataagctga gcaatcccaa tcgcgctcgt
     7141 cccagtcggc gttgaacact tcgacgatct gcgcgacgtg gtagggattc tcggtgatga
     7201 cgccgtaatc gcgggtcagc gtgaagtatt tctccatgag attgaacgtg gcgacgagtg
     7261 cgactttttc gtcgaccacg atcgactttt cgtgcgtcac gtagaacttc gaactcgacc
     7321 acttcaccga gatcccggcg tcctggaatt gctggtaggt ttcgtcattg gcgcgatcac
     7381 cgccggatcg tgccgcattg agcatcaccc gcacgtcgac gccggcgttc ttccgctcga
     7441 tgaccgccgc gatcaaactc ggttcggtga acgtgaattg cttgatcaat agcgaattct
     7501 gggccgaatt gatgaattcg cgtaccgggt ccaagccgtc gtcaggctcg actatcagac
     7561 gcggagagag cgggagcatg atttcgggat gctagcaccg ggacaatagc gtcgctttgc
     7621 tatcgcgtga acgttcagct gcccgtgaca cggcggtgat aagaccgccg ccacggtgat
     7681 accgaggtca gtcgggtacc tgccacatcc gcgacacgac ttcaggcaag attgcgtgca
     7741 tggacagtgg agcggcttcg ccccgcgtgc tcgtagtcga cgacgacccc gacgtgctcg
     7801 cgtcgctcga gcgtggcctg cggctgtccg gtttcgacgt cgcaacggcc gtcgacggcg
     7861 ccgaggcgct gcgcagcgcc accgagacgc ggccggacgc gatcgtgctg gacatcaaca
     7921 tgccggtgct cgacggcgtc agcgtggtga ccgcgctgcg ggcgatggac aacgacgtgc
     7981 cggtctgtgt gctttccgcg cggagctcgg tcgacgacag ggtggccggc ctggaggcgg
     8041 gcgccgatga ctacctggtc aagccatttg tgctggccga attggtcgcg cgggtcaagg
     8101 cactgctgcg caggcggggc gcgacggcga cgttttcgtc ggaaaccatc accgtcggcc
     8161 cgttggaggt cgacatcccc ggcaggcgtg cgcgcgtcaa cggcgccgac gtcgacctga
     8221 ccaagcggga gttcgacctg ctcgcggtgc tggccgagca caagaccgcg gtgttgtcgc
     8281 gggcccagct gctcgagttg gtctggggct acgacttcgc ggccgatacg aacgtcgtcg
     8341 atgtgttcat cggctacctg cgacgcaagt tggaggccgg cggtgcgccg cggctgcttc
     8401 acaccgttcg tggcgtgggg tttgtgttaa ggacgcaata gtccgatgaa cgtgctgtcg
     8461 cggttcatcc gccgcacacc ctcgctgcgg gcccgggtgg tggtcgccac cgcgatcggc
     8521 gcggcgattc cggtgctcat cgtgggagcg gtggtctggg tcggcatcac caacgaccgc
     8581 caggaacgct tggaccgcag gctggatgag gccgcgggtt tcgcgatccc gttcctgccc
     8641 cgcgggctcg acgaaatccc caagtcgccc aacgacaagg acgcggtcat cacgattcgc
     8701 cggcccgacg gccaggtcac ctccaattcg gacgtcgtac tgcccgcgtt gtcgcccggt
     8761 tacgaagaca cctatgtcaa cggcgtccga taccgcgtac gcaccgtcga aattcgcggt
     8821 cctcaggggc cgatggccgt cgccgtcggc gatacctacg acgcgaccat cgccgacacc
     8881 aacaatctgc accgccgggt gatcatcatc tgcttctttg ctgtcggcgc ggccgcggtg
     8941 ttcgcctggg tgctggccgc attcgcggtc caaccgttca aacagctggc gcaacaaacg
     9001 cggcagatcg acgcgggtga cgaaccgccg aacatcgaag tgcgcggcgc taccgaggcc
     9061 gtcgagatcg ccgacgccgt caagggcatg ctccagcgca tctgggaagc gcaggaccgt
     9121 accaaggccg cgctcgcgtc ggcgcgcgac ttctccgcgg tgtcggcgca tgaactgcgc
     9181 acaccgctga ccgcgatgcg caccaacctc gaagtgctct ccacgctcga cctgccggag
     9241 gagcaacgca aagaggtggt caacgacgtc atccgcaccc agtcgcgcat cgaggcgacg
     9301 ctcggtgcgc tggaacggct tgcgcagggc gaactgtcga cctccgacga ccatgtgccg
     9361 gtcgacatca ccgaactgct cgaccgcgcc gcgcatgacg cgatgcgggt gtatcccgat
     9421 ctggacgtgt cgctggtgcc ggcgccgacg gtgatcatcg tcgggctgcc tgcagggctt
     9481 cgattggccg tcgacaatgc gatcgccaac gcggtcaaac acggcggcgc gaggcgggtg
     9541 cagttgtcgg cggtcagctc gcgcgccggc gttgagatcg caatcgacga cgacggcgtc
     9601 ggcatccccg aagaagagcg caacgtggtg ttcgaccggt tctcccgcgg atcgacggcg
     9661 tcgcactccg gctctgggct gggcttggca cttgtcgctc agcaggccga attgcatggc
     9721 ggcacagcgt ctttggagga cagcccgctc ggcggagcgc gccttctgct gcggctgccc
     9781 ggaccgcgct aatccgccgc gcagtcgcca agactgctgt cctacacaag tagcggcttg
     9841 ccagcgcagc ggcggcgggc gttgaattgc ggtgttggac cacgaaacgg gggcggtcac
     9901 atggccaact acatcgcgct aatcagctgg acagagcagg gcgtcaaggc atacgggaac
     9961 acgctcgacc gagcagaagc cgctgccgaa ttggcgggca agctgggtgg ttccctaagc
    10021 cagatcttct ggacgatggg cgaatacgac gtcgtcggcg tccttgaggc gcccgacgac
    10081 gaaagcgcaa cggcgttcgc gcttgccctc tcgtcgcagg gaaacgtgcg cacatcgacc
    10141 atgcgtgcgt tcgacgccgg cgagatgcgg caaatcctcg ccaaagtgcc cggttagcga
    10201 gtgccgagca ggttcgatcg cgaggatcgc gtcatcgggt ggccagcagg tcgatgacaa
    10261 aaatcaaagt tttgccggag agccggtgcc cgccgccggc cgggccgtat gcctgttccg
    10321 gcgggatggt cagctgacga cggccgccga ccttcatccc ggggatgccg tcctgccagc
    10381 cctggatcag gccgcgcagc gggaactcga tggactcgcc gcggttccac gagctatcga
    10441 attcctcgcc ggtgtcgtac tcgacgccga cgtaatgcac ctcgacggtg ccgccgggta
    10501 ccgcctcggc tccgtcgccc accaccaagt cgttgatgag aagttcggcc ggggcgggtc
    10561 cgtcgggaaa ctcgatctct ggcttggtac tcatcgcact caccgtagtc tggattcgtg
    10621 cccaaggacc aagacattct cgcgcaggtc aacaagctgg tcgccgagga gcaggagctg
    10681 cgatccaagc tgcagcacca cgaaatcgac gaatccgagg agcatcagcg gctacgcgcc
    10741 gtggaagtcg aactcgacca gtgctgggat ctgctgcggc aacggcgggc gctgcgggag
    10801 accggtggcg atccacgaga ggcgcgggta cgcccgcccg gtgaggtcga gggctacctt
    10861 ggctgaccgg tgaccgagcc cgacgtcgtc atcgtcggcg gcgggcacaa cggcctggtc
    10921 gctgccgcgt atctggcccg tgctggccgc cgcgtgcagg tgctcgaacg gctggaccac
    10981 gtcggaggcg ccgcagtgtc cgcgcacgcc ttcgacggca tcgacgcgcg gctgtcgcgc
    11041 tactcgtatc tggtcagcct gttgccgcga cgcatcgtcg acgaccttgg cgcgcgggtg
    11101 cggctggtgc gccgccgcta ctcgtcgtat acacccgatc cagcgacgtc gggccggacg
    11161 ggactgctga tcggaccgca gtcaacgttc ggcgccgttg gcgccgcgga tgacgaggcg
    11221 ggattcgcgg agttctaccg acgctgccgg gttgtcacct cccggctgtg gccgacgttg
    11281 ctgcagccgc tgtgcacccg cgacgaggcg cgccggcatg tcggcgacga cgccgcgtgg
    11341 cggttcgtgg tcgacgagcc gatcggacgc gcgatcaccg aagcggtttc caacgatctg
    11401 gttcgcggtg tgatggcgac cgacgcattg atcggcacgt tcgcccgcgc cgacgacccg
    11461 acgctgatgc aaaacgtctg cttcctgtat cacctgctgg gcggcggcac cggcgactgg
    11521 gatgtcccgg tcggtggcat gggcgctgtc agcggtgcgc tggccgcggc ggcggcaggg
    11581 tacggcgctg aaatcctcac gggcgcagat gtttatgcca tcgacccgga cggagaagtg
    11641 cggtaccgcc gcgacggcca ggaccatcgg gtgcacgccg atgtcgtgtt ggccaacgtg
    11701 acaccggtcg tgctcgcgaa gttgttgggt gagccggagc cgacgatggc cccgggcgcg
    11761 caggtcaagg tcaacttgat gctgcggcgg ctccctcggc tgcgcgacgg cagcgtcaca
    11821 cccgagcagg cgttcggcgg cacgttccac atcaacgaaa cgttcagcca gctcgacacc
    11881 gcctacaccc gagccgatca cggtgtggtg ccggatccgc tgccgtgcga gatctactgc
    11941 cattcgctga ccgacccgag catcttgtcg gatcggttgc gcgactccgg tgcgcagacg
    12001 ttgacggtgt tcggcctgca caccccgcac agcctggtcg ctggcaagga tcccgatcgc
    12061 acgcgggact cgctgacgtc tgcggtgctg acttcgctga attccgttct tgccgaaccg
    12121 attcaagatg tactgatgga agactcagcg ggccggttgt gtatcgaggc caagacgact
    12181 ctggatttgg agcagacgct ggggatgacg ggcggcaaca tcttccacgg cgcgttgtcg
    12241 tggccgttcg ccgacgacga cgagcctttg gagacacccg cgcagcggtg gggtgtggcc
    12301 accgcccacg accggatcat gttgtgcggc tcgggatctc gacgtggtgg ggcggtgtcg
    12361 ggcatcggtg gtcacagcgc cgcgatggcg gtgctggaga gctaggacat cacatcgttt
    12421 cgcagctgaa ccacataccg agaactgccc gcgtaggcag cgacaatggt ggtgtcgctc
    12481 gctaggcggg cactctcaaa tgtctctcgg gtgccgctgc tgacacattc ccgcggccac
    12541 cgctatttcc cgagcaggtc gatgatcgcc ttcagctcct tgagctgggt gtcgtcgagc
    12601 gccgccaggg ccgtgggtgc cggatcgtcg accttatcga tcttgcggac catctcgcgg
    12661 ccggcgtcgg tcaatgaaac gaccttgcac cgtcgattgg tcggatcggt ttcgcgcacg
    12721 acgagcccgc ggtcttcgag atcgttgacc gctaccgtgg ccgcaggcgc atcgatggtc
    12781 gccgcgcgcg ccacctgctt gacggtcatc ggttgccgac tgagccgctt gaggatgcga
    12841 attctgctga acggcagtcc cgaagcctcg acgaccgctc gcttccaccc gtcgcggttg
    12901 tcgtgaacca gcgaggccat cgcccgccaa acctcgtcgg ccagaccatt acttgacatc
    12961 ggcagcctcg tagcgcacat caggaccagc gatcaacggc gccagccgct cagccgagcg
    13021 cgccgcccgc gccgacgtgg agtacacggc aagaacaatg atcaccaggc cgagtcctgc
    13081 acagatcaac cacagtggcc tggccgcaac agcaaagtcg acgcccgtcg tggccaacgc
    13141 cgaacccacc accgaaccgc atagcgccac accaacactc acgcccacct gccggctcgt
    13201 cgacgcgacc gccgacgcgg cgccggcccg atcggtcggc atgccactga ccgccgcgtt
    13261 ggtgatcggc gcgttcacca tcgagaagcc gatgccgaac accgcgaaga tcgccaacaa
    13321 ctgccacacc gcggtcgtca cggtcagctg ggtgagcaac agcgtcgccg cagtgatcag
    13381 agtcccggcg atcaggagcg acggcctggc gccgaagcgg ccgaccattc ggcccgatag
    13441 cggcgaaaag atcagcgcgc caacggcaac gggcaagtag atcagtccgg tgtgcatcgc
    13501 cgagaagccc cgttcgctct gcaggtacag cgacatcacg aacaggaacg cgccccaagc
    13561 cgcgaacgca cacaccgcga tcatcgtcgc ggacgcgaac ggaatgctgc ggaagaaccg
    13621 gagatcgatg aagggatcgc ggcggcgcga ctcgtaacgc aagaacgcga cgaacgcgag
    13681 tagtgcgacg atggcgacga ccatgttgcg ggcgtcggtc caaccccgcg ccgggccttc
    13741 gatcagcacg tagacagtgc cgaacaggaa tgtcatgccc aggccttggc caacggggtc
    13801 gacgtcgcgc atcgtcaccg accgagattc gggaacgaag atggccgtca gcacgatcgc
    13861 ggccgcgcag atcggcaggt tgatccagaa cacggctcgc cagttgacga gttcgatcag
    13921 cgctccaccg acgattgggc cgagcgccat cgagatgccg acgaccccgc cccagacgcc
    13981 gatcgcacga gcgcgttcca cccgcccggt gaacacctgc gtgatgatcg acatggcgac
    14041 cgggttcagc atcgaacccc cgatggcctg cagcatgcgc gcgagaatca ggatctgaac
    14101 attcggcgac aggctgcaca gcagcgagcc gagcgcgaag accgtcagcc cgatctggaa
    14161 ggtgcgcctg cgcccgaacc ggtcggcggt ggcgccggac agtagcagca gcgacgccag
    14221 caccagcgtg tagatgtcga tcacccactg cagctgtgac gcggtcgcat gcaggtcggc
    14281 ccggatgttc ggaatcgcga cgttgacgat cgtcgcgtcc atcgacacga tcagcaggct
    14341 caggcagcag gacaccagga tgatgccctt gcgccgagcg ctctggggcc cccgcgaagc
    14401 tggggacgat cggacgacag tttcattcac acaactattg tgaaactaca actgtctccg
    14461 aggcaagcgc ggacgccgtg agctgcgtcg cagtacgtca gcgcccgtcg agtgccttgt
    14521 agtcccgctc ggtgtagccg gtgtagacct ggcgcggccg gccgatcttg ccgctgccct
    14581 cgtcgtgcat ctcgcgccag tgcgcgatcc agcccggcag ccggcccagc gcgaacagca
    14641 cggtgaacat ccgcgtcggg aagcccatcg cgcggtagat cacgccggtg tagaagtcga
    14701 cattcggata cagcttgcgc tcgacgaagt agtcgtcggt cagtgcgacc tcttcgaggg
    14761 ccttggcgat atcgagcaat tcgtcgtcgc cgccgagctt gccgagaatc ttgtcggcct
    14821 gctccttgac gatgcgggcg cgcgggtcgt agttcttgta cacccggtgc ccgaagccca
    14881 tcagcttgac gccgtcttcg cggttcttga ccttcttcac gaagtcgtgg gtgtcgccgt
    14941 cggactgccg gatcttgtcg agcatctcca gcaccgcctg gttggcgccg ccgtgcagcg
    15001 ggccccaaag cgcgttgatg ccgcccgaga tcgacgtgaa caggttggcc tgcgacgagc
    15061 cgaccagccg caccgtcgac gtcgaacagt tctgctcatg atcggcgtgc aggatgaaca
    15121 acatgtccag cgcccgaacg agttcgggat cgacctcgta gggctcggcc gggaagccga
    15181 acgtcatccg caggaagttc tccaccagcg tcagcgagtt gtccgggtag aggaacggct
    15241 ggccgaccga cttcttgtac gcatacgccg cgatcgtcgg cagcttggcc agcagccgga
    15301 tggtggacag ctccacctgc gcgtcatcga acgggtcgag cgaatcctgg tagtacgcgc
    15361 tgagcgcgtt gaccgcgctg gacagcaccg gcatcgggtg cgcattgcgc gggaagccgt
    15421 cgaagaagcg cttcaggtcc tcgtgcagca gcgtgtgccg ctggatctgg gtggtgaact
    15481 tctcaagctg atccttatcc ggcagttcgc cgtagatcag cagatagctg acctcgatga
    15541 agttcgactt ctcggccagc tgctcaatcg ggtagccgcg atagcgcagg atgccggcgt
    15601 caccgtcgat gtaggtgatg gcgctcttcg tggaggcggt gttgacgaag ccttcgtcga
    15661 aggtggtgta tccgctcttg gacaacagcg agcccagcgc gatgccgtcg gctccctcgg
    15721 tggcgccgac aatgtccaaa tcgagttcgc cacctgggta ggagagggtg gcgtgctctt
    15781 caccactgga ggggttatcg gccacgggaa tcccttcgtc gctgccgtca atgcggaggt
    15841 ctgccctaca aaaggtagtc ccattccagc agtcgtgccc gcgcgggggc aggcagttag
    15901 ctagacgggg tgccgagcgt tctcgatttc acccatgaac tcggcgaacg tcgcttccaa
    15961 tcgcgtgatg atgtcgtcga tgccgacttg cgtccagtcc gttccttcgc caaggtcact
    16021 cagggcggtc atgatgatcg acgcccagac ggcggcgacc agccgtagcc ggcggtcata
    16081 gagttcgacg cccattcgct tggccagctc gatggtgacc ggatgcgggc ggaattcgac
    16141 ggcggcctgt cgtaatgcgg ccgaggacaa gacgatccgc acggtgcaca tcatccggtc
    16201 gaccgacagc ccccctggcg gtgtggtttt cgtgccgttg tacatggcga catacgagcg
    16261 gcgcagcgct tcgaagtggc tgatgtcgtg gggttgacga gcaagttcgg ccgcggtcaa
    16321 ctcgagtgcc tcatcgatca atgccagcgc gatggcgtcc ttggtcggga agtaccggct
    16381 gaacgtgcgg ggcgaaacgt ctgcgatggc ggcaatctgg tcgacggtcg tgcgctcgaa
    16441 accctgccgc tcgcacagct cgacggcggc gtcgatcagg gtggcgcggg tgcgctgttt
    16501 cttgcgttca cgcaatccga gggccggctc cccccttact tcagccacga aggggatgct
    16561 aactccgcac gctatgaatt ggattagaaa gtggcaaagt cagacacttg gcgtcgctca
    16621 cactttgtcg ccttcggcca cccggtagcg gataaacgca ggcaccgtca gagcggcgat
    16681 caccacacca accaccacca gcacaccacc acccgcggcg gctaccgtcg tgcccaccac
    16741 tgctgcggcc gcgccgtgca ccgcgtccgc caaccgggga ccgccggcca cgacgaccgt
    16801 gaacactccc tgcagccggc cgcgcaagtc atcggatgcg gcctgttgca ggatcgtcga
    16861 ccggaacgcg gccgacacca tgtctgccgc accgccaatg gccaagaacg ccaatgcaat
    16921 ccacagcaac gccccggtgt ggccatgcgc aagcccgcct gccaggccga agccgatcat
    16981 ggccccgccc cacacgacga tcgaaacgac caccgcaagc ccctgacgct ggatgcgggg
    17041 aaaccagccg gagaacactc cgcctgcgac ggcgcccgcc gacatcgcgg cggcaagcag
    17101 cgccatcgtg gtgccgccct cgaccgggcc cccgaaactt tcgtgcgcca tttgcgggaa
    17161 caaagctcgt ggcatgccga ggatcatcgc gatgaggtcg acgacgaacg acatcagcac
    17221 cacccggttg ccggccaggt agcgaaagcc gtcgagcacc gcggtgaatc cgtagcccga
    17281 tgggccgttg ccgccgttgg tgggtggcat cggcgcgagc cggaaggtcg cccagatcgg
    17341 cgcaatgcac gtgatcgcgt cgatcagata cagcgtggac aggtccaccc agtgcagcat
    17401 cacgccggcc agcagcggtc cgacgatcgc gccgaactgg aagaccgtga aattcagtga
    17461 gttggcggcg gcgagttggt cgccggggat catgcgaggg atcgccgccg accgagtcgg
    17521 gctgttgatc gcatagaacg cctgctgcac cgacagcagg cacagcacca cccagacgtt
    17581 gttcagggcc agcgcggcct gcagccacag catcaccgac gacactgcga gcccgatcga
    17641 cgcgatgatg agcagcgttc gccggtccat cgcgtcggcc cacgcgccgc ccagcagtcc
    17701 gaacacgatc agcggaacca gtgcgaataa cccggacagc ccgacgtacg cggagttctg
    17761 cgtgagcgcg tacagctgga ccggcaccgc gaagatcgtc aggttgccgc cgatgaccgt
    17821 gacaatcccg gtcagccaca accgccggaa gtccggtgtg cgcagcggtg tggtgtcggc
    17881 gaagaacttc cgcactacgg ctggagccgc tcgatgcggc cctctgataa gcgaatcctg
    17941 ttgtgtaccc ggttttcccg gccctgccag aattcgacga cctcgggcgc gatcagatag
    18001 ccaccccagt tcggcggcac cggcacctcg tcgtggtcgg cgaatcgttc ggtcacgtcg
    18061 gccaactgtt ccagcagcgc cgcgcgtgag gcaatcggtt ttgactggtg cgacgcccat
    18121 gcgccgagct gcgagccgcg cggccggttg gcccagtagg cggcagttgc ctcggcggac
    18181 accttggtca cggggccgcg cacgtggacc tgacggccaa gcagaaacca cgggaacgtt
    18241 gccgacgcgt acgcgttcgc ggcaagctgc tcgcccttgg cggagtcgta gttggtgtag
    18301 aaggagatgc cggtttcgtc cacgctcttg cacaacacgg cacgagtcac aggccttccc
    18361 cgctcgtcga ccgtcccgac caccatcgcg ttcggctcgg caatgccggc gccctcggcg
    18421 tcggctaacc acttgcgcag cagggcgacc caaccgtcgg caagccagtc cgcatcgaga
    18481 tccgggctgc cgtccttctc gacggatccg tactcgacac gcattctcac taggtgttcg
    18541 tcgtcacggg ttgccacgcg acaacgctac gcttccgctg ttcgccgact tgttaccgac
    18601 cggtagacaa ggccgactac ccgggtgcaa gaatcggccc atgactgcgg taccggaaga
    18661 cttcgtcgag gggctcgaag gcgtcgtggc cttcaccacc gagatcgccg agcccgacaa
    18721 agacgggggc gcgctgcggt accgcggcgt cgacatcgaa gatctggtca accagcaggt
    18781 gacgttcggc gacgtgtggg cgctgctggt cgacggcaaa ttcggccaca gcctgccgcc
    18841 cgccgaaccg ttcccgctgc ccatccacag cggcgacgtc cgcgtcgacg tgcaggccgg
    18901 gctggccatg ctggcgccca tctggggcta ccccccgctc ctcgacatcg aggacgagac
    18961 cgcgcgcgag cagctggccc gcgcgtcggt gatggcgctg tcctatgtcg cacagtccgc
    19021 gcgcggcatc taccagccgg ccgtcccgca gcggcggatt gacgaatgct ccaccgtcac
    19081 agagcggttc atgacgcgct ggcaaggcga ccccgacccc aagcatgtcg aggccatcga
    19141 cgcctactgg gtgagcgcgg ccgagcacgg gatgaacgcc tcgacgttca ccgcacgggt
    19201 gatcgcgtcg acgggtgcgg atgtggctgc ggcgctgtcg ggcgcgatcg gtgcgatgag
    19261 cggcccgctg cacggcggcg ccccggccag agtgatcccg atgatcgagg aggccgagaa
    19321 gaccggcgac gcgcgtgcag tggtcgcggg catcctggac cgccacgaaa agctgatggg
    19381 cttcgggcac cgcgtctacc gcgccgaaga tccgcgggcc cgggtgctgc gcgccaccgc
    19441 caagcgactg gaggcgccgc gctacgaggt agcggccgcg ctcgagcagg ccgcgctcgc
    19501 cgaattgcgg gagcgccggc cggatcgcgc catcgagacc aacgtcgagt tctgggcagc
    19561 ggtgatcctg gacttcgcgc aggtgccgac gaagatgatg cccgcgatgt tcacctgtgg
    19621 ccgcaccgcc ggctggtgcg cgcacatcct ggagcagaag cgactcggca agctcgtccg
    19681 cccgtcggcg atctacgtcg gccctggtcc acgaagcccc gaagctgtcg aggggtggga
    19741 ccaagtcagc aagtcgtgac tgactccgtc gcgacattcg cttcggcggc acgcagtttc
    19801 gccgcgctgg tgcatgagat ccccgcctcg gcatgggacg gcccaggtct tggtgaatgg
    19861 gatctgcgct cgctggtcgg acatgcgtca cgttcgctca tcacggtctc cacgtatctg
    19921 cagcagcccg cagaccgcga ggacatcact acgccgcagg agtactacgt gagggtgaac
    19981 cccgcgacgc tcggtctcgc gccggccggg gtcgtcgagc gcggcagaca ggcggggcga
    20041 gatctcggag acgatccggc cgcgagggtc gatgagctgc tgtcgcaagt gctcggcgag
    20101 ctgtcaggtg tcgatgaccc gttgatcacc gtcatcggcg gcgcgggcat ccggctgcag
    20161 acgtatttgc cgacgcgggt gtttgagctg gcggtgcacg gggtggatat tgctcgtgcc
    20221 gttggcattt cgtatgcgct gccgaccgac gtgctcgagg aggcgaccgt gttggctgcc
    20281 cgcatcgcgg gcgccgaagg gcacggtgag gcggtgctgc tggcgttgac cggccgcgat
    20341 ggcttgccgc cttcgttctc gattgtctga gttcacccac ttgtcggtgg ggcgtgcttg
    20401 ggtggacggc atggcaatcg aagtacaacc ctcagtttcc ccgcacctga ccgttggcga
    20461 tggcgccgct gcaattgact tctatgtcaa ggcttttggc gctgtcgagt tgggccgggt
    20521 gcccggtccc gacggcaaac tcatgcacgc cgcactgcag atcaacggct ctacggtgct
    20581 gctcaacgac gacttccccg agtacaacga cggcaagtcg tcgacgccga ccgcgctcgg
    20641 cggtacaccc gtgaccatcc acctgacggt caccgatgtc gactcgaagt tccagcaggc
    20701 cgtcgatgcc ggcgcgaccg tcgtcagtgc actcgaggac cagttctggg gcgaccgcta
    20761 cggcgtgttg cgtgatccct tcggtcatca ctggtcactg ggtcagccgg tgcgcgaggt
    20821 gagcccggag gaaatcgcag aggcgatgaa gcagcactaa ctgcccggag gaccacacct
    20881 tctcggggcg tcatcaattg ttgacgctcc gagaggcggg accggcctac acagtcaggc
    20941 ccgggatgta catcgccagc acgctcagcg gcaggctgtt ggtctccgcg atcccgctga
    21001 tcgcgtcgat gctggcctgc ttcggcgtgc gctcgagttc gggcgtcgtc gggtcggacc
    21061 cgtacagccc gaactcgagg tcgtagccct cgacccactc gagattgtcc acgaacgacc
    21121 aataggtgta gccgcggatg tccatgccgt gggtgaccaa gtcttggacc accccgatgt
    21181 ggctgacgat gtacggcggt cgcttggagt cgcccgcgtc ggcaatgcca ttctcggtga
    21241 cccacaacgg ctttccgtat gaggctgcaa tctccaggac ctcgcggaaa ccgccgggat
    21301 cggtgggttg gttgaagtcg ctgcacgtcg gctccgaggc cgcgcatcgc agcggaaggc
    21361 cctttagcca ggggaaaccg gggatcggcg cgaggccgaa gccctgcatc ggttgtgacc
    21421 cgtagtactg caccccgatg aagtcgacct tctgcgccat ctcgggatga agctcgtcag
    21481 gcgtttgtat gccatccaga ttggcgtcga cccacccatt tacgacagcg ttcgggaacc
    21541 agcggttgta gaagttgttc cacgcatcgg ccgcctgtac atcgagggga ttcaccggat
    21601 tggccggccg ggccgggatc atgttgttgg caaagcccac gaaagcggtg tcgtcccagg
    21661 catgtatcgc gtcgtacgcg gcgacgtagc ccttggcctc gttgacgagg aacgtcgagg
    21721 cgagatcggg ccggatcagg cccggcggcc agttcggaac caggctcggt atcgccagaa
    21781 actcggtgag taccggcggc acgggctcgt tgagcaccgt ccagttgtcg acctggtcct
    21841 tgtacttcca cgccacatac gcggcgtact tctcgaattc gaccggcgtg tccggcgaaa
    21901 gccatccggc gtcaggcgcg ggcagcccga gctgggccag cggccgggtg gtgatcgggt
    21961 cgtgtatcca ggcgggcaac gtgaagtggt tgacggtgat caacggttcg aggtggtgcg
    22021 cgatcaagga atcgaacacg tcgctgtaat gctggacctc cgcctggttg gccagctcgt
    22081 ccagggcctc caaatcgtcc tcggtgatta ccccgccttc atccgagata tcaacggatg
    22141 ccgtggaatt cgggaaaata cggctccact cgatactcat ccggaacgtg ttcatgccga
    22201 gttcagtttc cgcgagcgcc gcatcactgt cgtacttgac gtaggtgccc ggcccgttct
    22261 ccggcactcc gttggagatt cccaacagct ggttgatcgg gtcgtgcacc cacttgtacc
    22321 aatcggaatt cggatccacg ggtgaaccgg ggccaccctc ggcctggaag ccggcatgtg
    22381 ccacgcccca gtggaaggtc ggcgggaatg acaggttgac gcccgcaacc gggtcgacgt
    22441 tgacggtgat cgtctgcgcc actgcgtcgc cgccgccggg attgatgaag ttacccagaa
    22501 tcggcacgaa cttgagcagt cccagcgggc cgtgcacatg caacccggct gtctcgtcgc
    22561 tgacaaccac ggtgaatgag tcggtccccc cgaccgcagc catcgcattc atggggcggt
    22621 agatgaagtt gccgtcgtcg tcgatctcca gcgttccgcc gttgtgtggc cggccgatga
    22681 cggtgtacgt cagcgggtcg tcgtccgggt cgaccgcacc gaggttgccg gtaatcagcc
    22741 cgttcgtttg cgggttgatc accgggttga tggtgggcga tccgttgaac aactcgcggc
    22801 gtacccatgc cagcagcgtc cacagcgatg gcggatcggc gggcggagtc ggggcgcccg
    22861 ccgcgaacgg gctcaggaac gcgctgacga cgctcgagac caagccgccc acgtaggtga
    22921 ccaaggtgcc cgggtcgaag gccgttggcc aaggcctcag ggacggaacg gtcggcggcg
    22981 tcagtgccgt cgtcaccgca gcagattctg tgtccgcggt cgacgacatc gtctgtaccg
    23041 caaaggaact aacggcgatc cgggcctctg cccccgactt gagttccggc tccggatcgg
    23101 tgtcgacctt cacttcgaca gcctcgacct tgctgtcggg tgcagacgtc ttggtcgatt
    23161 ccgccgtcac gtcggctgcc ttgccggagt ccggcttctt cgtcggctcc gttgtctcct
    23221 cgtcgtcctt ggacttcgaa gcgctctgtg cgccgccggt gctgactacg acaccgggcg
    23281 gggcttgggt cgtcgtcgac cctgtcgacg acgttggctc cgtcgacgat ggctgcgtcg
    23341 gttccgtcgc cgttggcttc tcggctgatt ctgccggggc ggatgcttcg gtggatgtcg
    23401 acgtatcggt ggacgacgac tccgtcgatg acggctccga ggacggcgta tccgcccacg
    23461 ccaccggcaa cccgtacccg gtgaccaccg caaccccgac ccccagggct acggctaacc
    23521 cccctacccg accgacgtag gcagcagcgc gcatggacac tgcatacgcc cgttgtcgcg
    23581 gaggtagggc gtatttcgag aaagtgcgcc aattagcgaa gcaacccggc gagcagtcgc
    23641 tggtgtactc gtggcgtggg cgccgccgcg gcggccgcca gcatgtcggc gaaagtggtg
    23701 aacttgtcgc gcggccggtc ctcgccgccg cgggcgatct ccgcggcatc gatggccttc
    23761 cagccctccg cgtccaccac cgcgggctgg cgggtgcgcg ccagtttgtc caacgctgca
    23821 ggcttcccga tggggtcggc cagcaggccg tggttgtagt cggcgaccag gttgtgcacg
    23881 gtctgcgccg cacacgactt gttggttccg atgaaaccgg tcgggccgcg tttgatccag
    23941 cccgcgacat agctgcccgg cgctccgatt acacggccgc cgtcgttcgg cactaccgct
    24001 gcggactcgt cgaacggcag cccgcgaatc ggcttgccgc ggtagccaat tgaggtcaac
    24061 accagaccgg ccgacaccag atggacctcg tcagtgccgg tcacggtgaa ttccacaccg
    24121 tcggcacatc ggtcccccac cacacgtttc ggcgtcaact ggtacgccag ccggatacgc
    24181 ggccgcgtca ccggcgccga cgcgtcaccg agcttgctca ggatctccaa cttgttgcgg
    24241 gtcaacggat ctgactcctc ggccagatca cgcaacacca ggtcgtgatc ggcggcgtcc
    24301 agcaccacct cgcacgtcga cgtcaggccg atcagctccg gcagcgtgaa agccgaatcg
    24361 gcgggaccgc ggcgggcggc tatcacgacc tcttggactc gcgaggtccg tagcgccgcc
    24421 agcgcatggt cggagatgtc ggttcgggcc agcgcgtccg ggtcggtcgt caggatccgc
    24481 gcgacgtcca acgcgacgtt gccgttgccg attatgacca cccgttcgtg gctcaaatcg
    24541 actggcagat cggtgaattc gggatggccg ttgatccacg ccaccacttc ggtcgcggtg
    24601 ccggtgcccg gcagcccgat gccttcgatg tcgagtcgac ggtcgttggg cgcccccacc
    24661 gcgtagagca ccgcgtgatg gtgctcgagc agctcgtggt gagtcaggtc cgatccgacc
    24721 tcgacattga ggtagaaccg aaacccgcgc cgcctggcga tcctgtcgaa cagcttcgtc
    24781 acccgcttgg tgctctggtg gtcgggggca accccggccc gcaccaaccc gtacggcgtt
    24841 ggcagccgtt cgaagacatt gacccgcacg ccctgctggg tgagcagttc gtcggcggca
    24901 tacatcgcgg caggcccgga gccgacgatg gccaccgtca gcgggccgcc cgcgcgtgcc
    24961 tcgacgaacg gtgcctcgat caccggcgcc agcttggacg tgggcggcag cttcgattcg
    25021 cgcttcggat agaacgcggc gttcagctcg atgaacggca gctgcttggg cttgagcttg
    25081 gtgtccggtg cgatggcccc gaccgggcac gcgctgacgc acgcgccgca gtccacacac
    25141 gccgccggat cgatgtagag catctcggcg gtggcgaagc ccggctcgtc cggtgacgga
    25201 tgaatgcagt tcaccgggca ggcgtagaca caggacccgt cgctacagca cgactgggtg
    25261 attacgtgcg gcatatgact cctaggcggc ggccaccacg tgctgccgct gcggctcgct
    25321 gcggtagcgg ctgggccggc cgtcgatctt gcagatccgc cacaccagtt tggcgatcgg
    25381 gttgatcagg ccggtgtcct tgcagagcat ccggacgtcg ccgaacatgt cgcgcagcat
    25441 ctgccgggac tccggcgagc ggaagaacac ttccttgcgc accgagcgcg gaatgtcgaa
    25501 ttccttccag aacgccctcg gcggaacgat gatcgccgag cacaggatcc gcatcaccag
    25561 cggcacgtag atcgacagcc agaaccgctt gcgccgcggc aggtgcggaa tccgcttgcg
    25621 caggtactca tgcgcgaacg agatgtggcg agcctcctcg gccacgtgga tggccatcac
    25681 ccgttccatg atcggatgca gcgacttgcc ttcgcgcagc acgtttttct gcgtgtggtc
    25741 gatgggctcc tcgccggcga ggatgccgaa ccaaaacggg atcggcagcg ggccggccac
    25801 caacgggatc aacggctgga cccacttcag catccgcggc atgccgggca cgtcggcgcc
    25861 gatgcgattg accatctcct gaaacatcat ggtgtggttg cactcttcga ccgcctcgtg
    25921 caggcagtac cggtactccg gtgagccgtt cggcgtccag aacgagtagt ccatcaggcc
    25981 acggatcagg atcgactcga agtgcagacc gaccttggcg acgttggcct ggcgccacat
    26041 accgatctcg
//
LOCUS       DASRJA010000694         3298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1178736,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000694 DASRJA010000000
VERSION     DASRJA010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178736"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VFC01_31270"
     CDS             <1..956
                     /locus_tag="VFC01_31270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN84135.1"
                     /translation="NGITLKVVVEGSGPLLLLLHGFPQSGYLWRHQIDELVAAGYQVA
                     VPDQRGYGGSDKPAEVEAYDLLQLSADAVGIADALGHETFTLVTHDWGAIVGWHVALL
                     YPQRVNAVFALSVPPTIGTPVGALTRQENFGDNFVYTVYFQQPGVAEAELDADVRKSI
                     RMLYYSVSGDAPAFGFMRPKPASSKMLDGLVDPDPLPSWLTDEDLDQYCEDYRDGFRG
                     PINWYRSIDRGIELTRHLAGTKITQPSHFMIGSRDPMNLLLADPLANLEHNAPNLRGN
                     VVLEGAGHWLPIERPKEVNTALLDFLAGLASDPPSLSITKE"
     gene            964..2448
                     /locus_tag="VFC01_31275"
     CDS             964..2448
                     /locus_tag="VFC01_31275"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN84136.1"
                     /translation="MASRTGGFAPPLNQIAFSVVDLRLTERWFREGFGLLPAGGSRAL
                     MRGPVAANVQGLPRAASTCWWLLGRNSWFQLELFQFERPIANLKPADFRPCDIGYTRI
                     GIWVDDFDATLKRLAGLGSQPLTEPSGERGERRACVRNPDGVYVEIMEDDPLPHVNQR
                     GRADCPSAIRSVTVSVHQLSDAAAFFETGVGLKPSTVALHTPEHEALWGLAGATADSR
                     LFDGGEVLIEVVQYLDPAGKPRPSDYHLSDQGILNIAFGARRKADFNAVYRRAEAFGA
                     KPNCRPVHLPGAGSVVYVNDPHGLSVEILRTKSGLADRIFGFEPRPMHKRPAPDTRRI
                     EHRVNINAPVDVVWEAITDQDSMAEWIGFDPVTVRKEGWTQRHGAGSERLMQGPRGVG
                     RIVEQVIATSSQQSLRYRVIESSPLTCHQGEITLKQSDGHTELHWVIRFRPKLAGTGA
                     LLQKVLQAKLLSMLDGRLRPYIETSTAAGVFTTGGSENAETGVR"
     gene            2445..3298
                     /locus_tag="VFC01_31280"
                     /pseudo
     CDS             2445..3298
                     /locus_tag="VFC01_31280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305615.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
BASE COUNT          630 a         1077 c         1014 g          577 t
ORIGIN      
        1 ccaacggcat cacactgaag gtggtcgtgg aaggcagtgg cccgttactg cttctgctgc
       61 acggctttcc gcaaagcgga tacctgtggc gccaccagat cgacgagttg gtcgccgcgg
      121 gctatcaggt tgccgtgccc gatcaacgcg gctacggcgg cagcgacaaa ccggccgaag
      181 ttgaagccta cgatctgctg cagctgtccg ctgatgcagt cggtatcgcc gatgcactcg
      241 gacacgagac gttcaccttg gtcacccacg actggggcgc gatcgtcggc tggcacgtcg
      301 cgttgctgta tccgcaacgg gtcaatgcag tgttcgcgct gagcgtgcca ccgactattg
      361 ggacacccgt cggcgccctg acgcgccaag agaatttcgg cgacaacttc gtctacaccg
      421 tctatttcca gcagcccggt gtcgcggagg ctgagctgga cgccgacgtg cgcaagtcga
      481 ttcgcatgct ctattactcg gttagcggtg acgcgccggc gttcgggttc atgcgtccca
      541 aaccggccag ctcaaagatg ctcgacggct tggtcgaccc tgatccgctg ccgagctggc
      601 tcaccgacga ggacctcgac cagtattgcg aggattaccg cgacgggttc cgcgggccga
      661 tcaactggta tcgcagcatc gaccgcggca tcgagctcac ccgtcacctc gcgggcacca
      721 agatcacgca gccgtcacac ttcatgatcg gcagccggga cccgatgaac ctgctactcg
      781 cggatccgct tgccaacttg gaacacaacg caccaaacct gcgaggcaac gtcgtcctcg
      841 aaggtgccgg acactggctt cccatcgagc gaccaaagga agtcaacacg gcgttgctcg
      901 acttcctcgc cgggctcgcc agcgatccgc cgtccctctc catcacaaag gaatgagacc
      961 gagatggcct caaggaccgg cggcttcgcg ccgccgctga accagattgc cttttcagtg
     1021 gtcgatttgc gactcaccga acgttggttc cgcgaaggct tcggcctgct accggccggc
     1081 ggcagccgcg cgttgatgcg cggcccggtg gcggctaacg tgcagggcct accccgggcg
     1141 gcctcgacgt gctggtggtt actgggccga aattcctggt ttcagctcga gctgttccaa
     1201 ttcgaacgcc cgatcgccaa cctgaagccg gcggatttcc ggccctgcga catcggctac
     1261 acccgcatcg gtatctgggt cgacgatttc gacgcgacct tgaaaaggct tgccggactt
     1321 ggcagtcagc cgctgactga gccgtcgggc gagcggggcg agcggcgcgc ctgcgttcgc
     1381 aatcccgacg gcgtctacgt cgaaatcatg gaagacgacc ccctgccgca cgtcaaccaa
     1441 cgcgggcgcg ccgattgccc gtcagcaatc cggtcggtga cagtgtcggt tcaccagtta
     1501 tccgatgctg cagcgttttt cgagacaggc gtcggcctca agcccagcac cgtggccctt
     1561 cacacgcccg aacacgaggc gctgtggggg ctcgcgggcg ccacggcgga cagcaggctg
     1621 ttcgatggcg gcgaggtgct gatcgaggtg gtgcagtacc tcgatcccgc tggaaagccg
     1681 cgacccagtg actaccacct gtcagaccag ggcatcctca acatcgcgtt cggggcgcgc
     1741 cgcaaggcgg acttcaatgc cgtctatcga cgggccgaag cattcggggc caagcccaat
     1801 tgccgacccg ttcacctacc cggggcaggc tctgtggtct acgtcaacga tccgcatggc
     1861 ctctcggtcg agatcctgcg aaccaagtca ggactggccg accggatctt cggattcgag
     1921 cctcgaccca tgcacaagcg acccgccccg gatacccgca gaatcgaaca ccgtgtcaac
     1981 ataaacgcgc cggtcgacgt cgtgtgggag gcaatcaccg atcaggacag catggccgaa
     2041 tggatcggtt tcgacccggt cacggtgcgc aaggaaggct ggacgcaacg gcacggcgcc
     2101 gggtccgaac gcctgatgca aggcccgcgc ggagtcggcc ggatcgttga gcaagtgatt
     2161 gccacgagct cacagcaaag cctgcgctac cgagtcattg aaagttcacc gctgacatgt
     2221 caccaaggcg agatcacgct gaagcaatcc gacggtcaca ccgaattgca ttgggtcatc
     2281 cgatttcggc ccaagctcgc cggtaccggc gcgcttctgc agaaggtatt acaagccaag
     2341 ttgctaagca tgctcgacgg acggctcagg ccgtacatcg aaacatccac ggctgctggt
     2401 gttttcacga caggaggttc ggagaacgca gaaacgggtg ttagatgagc gcgaatccac
     2461 ccggcccccg caatgtgggc atattgatca tcggggccgg cttctccggg cttggcgccg
     2521 ccatcaccct ccaaaaggac ggctacaccg actttctggt gctcgagcgg ggagacgacg
     2581 tcggcgggac ctggcgggtg aacacctacc ctggcgcggc ctgtgatgtg ccgtcccacc
     2641 tgtactccta ctcgttcgcg ccgaaccgta cgtggacccg ttcgtactcc aaacagcccg
     2701 aaatcgaggc atacatccag caggtggctc gacggcatcg ggtgctggac agacaccggt
     2761 tcggctgcga ggttgacgag gtgcgctgga atgcccagga ggcgcggtgg cacgtgagca
     2821 cgtcggcggg ggtcttcacg gcgaatttcg tggtctcggc agtgggtccg ctgaccgagc
     2881 cggcactgcc ggacatcgac ggtctcgacg agttcgaggg cgagatcttc cacaccgcgc
     2941 ggtggaacca cgacttcgac ctgaccggca agcgcgtcgc cgtcatcggc acgggagcat
     3001 cggccatcca ggtcgtcccc gaactggcca aaactgtgcg gcagctggac gtgtaccagc
     3061 gcaccgcccc atgggtcctt ccccgtttgg accgggatta cacggccgcg gaccgcctag
     3121 ccttcgcgca cgttcccggc tatcagcgct tagtacgatg cgcgatgtac ttgagcaagg
     3181 agctcatcgg tttcgccttg acctatgcac ccaaaatgct tgtgccggta cagaaactag
     3241 ccaagccaca tcgagcgcgc gattgccgat ccccagctgc gcgccaaagt caccccgc
//
LOCUS       DASRJA010000695         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_94247,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000695 DASRJA010000000
VERSION     DASRJA010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94247"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFC01_31285"
     CDS             complement(<1..328)
                     /locus_tag="VFC01_31285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84137.1"
                     /translation="MFETLKLIVDAIRGALPALTKARKEKRLAQVGAALFLLYVKGNE
                     IVATGYRIVDFLENYVDRVQNGNAYTKAHAGRWISSTLQPLLRGQYHDLRDLDIAMGK
                     LQIELQI"
     gene            457..1779
                     /locus_tag="VFC01_31290"
     CDS             457..1779
                     /locus_tag="VFC01_31290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HZN84138.1"
                     /translation="MKTVPTLRVVSTTDTVEQARQMGVELPTEMQVTLANIGVVAREG
                     LLAMSVAAGMAVMQTLFDAEIAAVCGPKSRHDAERTASRHGRENGSVVLGGRRVPVTR
                     PRARTVGGHEVPLTTYALFAGEDQLSKVVLERMLAGLATRRHPAAAEPVGEAVEGTAT
                     ATSRSAISRRFVVATKTALAELLARDLGPLDIKVLMVDGEHIADHCCVVALAITADGT
                     KVPVGLWEGSTENKTVAKHLLADLVSRGLNTDDGLLVVIDGAKALSAAVTEVFGAKAA
                     IQRCTVHKRRNVGQHLPESERNWVDVKLVRALNNPDAAAGLRDAKALATALERKHPGA
                     AASLREGLPELFTVARLGITGTLATTLTSTNPIESMISIARTTNRNVTRWRDGQMVLR
                     WTAAGMINAQRSFRRVKGYKQMPQLVAALRRHAHPATPRTTGTVGTAA"
BASE COUNT          405 a          710 c          700 g          343 t
ORIGIN      
        1 cgatttggag ctcaatctga agcttgccca tggcaatgtc aagatcacga agatcgtggt
       61 actgtccacg cagcaagggt tggagtgtgc ttgagatcca acgccctgca tgtgcttttg
      121 tataagcgtt gccattttgg actctgtcga cgtagttctc aaggaaatcc acaatgcgat
      181 atccggtggc aacgatctcg ttgcccttaa cgtagagaag aaacaatgcg gcccccactt
      241 gtgcgagccg cttttctttg cgggctttgg ttaaggcggg taaggcgccg cgaatcgcat
      301 caacgattag cttcagtgtc tcaaacatag agactcctcg atgaggagcg tgtcccgatc
      361 ttcgtagaag ttgagtggcg gtcccccacc tgaggccttc cgagtggtag gtctccggtg
      421 aacccgccaa ggatcacgcg aaggaagggg accgctgtga agaccgtacc gactctgcgc
      481 gtggtgtcga cgaccgacac cgtcgaacag gcccggcaga tgggtgtgga actgccgacc
      541 gagatgcagg tgacgctggc caacatcggc gtggtggctc gggaagggct gctggctatg
      601 agtgtggccg ccgggatggc ggtgatgcag acgttgtttg acgccgagat cgccgcggtg
      661 tgcgggccga agagccgcca cgacgccgag cggaccgcgt cgcggcacgg ccgggagaac
      721 gggtcggtgg tgctgggcgg ccggcgggtt ccggtgaccc ggccccgggc ccgcaccgtg
      781 ggcggccacg aggtgccgct gacgacatac gccctgttcg ccggtgagga ccagctgtcg
      841 aaggtggtgc tggagcggat gctggccggc ctggccaccc gccgccaccc ggcggcggcc
      901 gagccggtcg gcgaagccgt tgagggcacg gcgaccgcga cgagccggtc ggcgatctcg
      961 cgccggttcg tcgtggccac caagaccgcc ctggccgagc tgctggcccg cgacctgggc
     1021 ccgctggaca tcaaggtgtt gatggtcgac ggggagcaca tcgccgacca ctgctgtgtg
     1081 gtcgcgttgg ccatcaccgc cgacggcacg aaggtgccag tcggtctgtg ggaaggctcg
     1141 acggagaaca agaccgtggc caagcacctg ctggccgacc tggtgtcccg cggcctgaac
     1201 accgacgacg ggctgctggt ggtcatcgac ggcgccaagg cgctgtcggc ggcggtcacc
     1261 gaggtgttcg gcgccaaggc cgccatccaa cgctgtactg tccacaagag acggaatgta
     1321 ggccagcatc tgcccgagtc cgagcgcaac tgggtcgacg tcaagctcgt ccgcgcactg
     1381 aacaaccccg acgcagcggc cgggctgcgc gacgccaagg ccctggccac cgcgttggag
     1441 cggaagcacc ccggtgccgc cgcctcgctg cgcgaagggc tgcccgagct gttcaccgtg
     1501 gcccggctcg gtatcaccgg cacgctcgcg acgacgttga ccagcaccaa ccccatcgag
     1561 tcgatgatct ccatcgcccg caccaccaac cgcaacgtca ctcgttggcg cgacggccag
     1621 atggtgctgc gctggaccgc cgccggcatg atcaacgcgc aacgctcgtt ccgccgcgtc
     1681 aagggctaca agcagatgcc gcaactcgtc gcggcgctgc gccgacacgc ccaccccgcc
     1741 accccacgaa ccaccggaac cgtcggcact gccgcataag atcagaacgg gccgtcattc
     1801 aaattccacg atccccgtga catcctcgcg tccttcgcgc cggcgatcat ctggctggcg
     1861 gcccagccga cgaggccgac cgttcccgac gccgcccatc agccggacac aaaacctaga
     1921 acccacgtga cgccctggca agactacgca tcgggcgcct aaggacaggc ccccgtttac
     1981 cccccgaaaa cccctgcgta cggctgacaa tcggtgacaa gtagcgatca tcggcttgac
     2041 gcgtttacac aggtcaatgc acgccttcga cagccaatga catgcctggg atggtcacgg
     2101 ttggtaggaa gaggtcgtcg gttcaaatcc ggccaccccg accaggcaaa ccagcacg
//
LOCUS       DASRJA010000696        18508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2653608,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000696 DASRJA010000000
VERSION     DASRJA010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18508
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2653608"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFC01_31295"
     CDS             complement(<1..518)
                     /locus_tag="VFC01_31295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HZN84139.1"
                     /translation="MTQAPDREKALELALAQIDKNFGKGSVMRLGEEVRQPISVIPTG
                     SIALDVALGIGGLPRGRVVEIYGLESSGKTTVALHAVANAQAAGGIAAFIDAEHALDP
                     DYAKALGVDTDALLVSQPDTGEQALEIADMLIRSGALDVIVIDSVAALVPRAEIEGEM
                     GDSHVGLQARLM"
     gene            752..1642
                     /locus_tag="VFC01_31300"
     CDS             752..1642
                     /locus_tag="VFC01_31300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN84140.1"
                     /translation="MTAPEDIRIPSHGEQLAAYLYRPESTQGDVPCVVMAHGFSATRD
                     DGLPGYAEAFRDAGLAVVVFDYRHFGASTGEPRQLLDIGRQHEDYRAVITWARELDGI
                     DPDRIVLWGSSFSGGHVLAVAAADPRIAAVISQAPFTDAIPTLMHVPLKNAVRLTVAG
                     IRDQLGGWRGRALRLAPAVGEPGTLAAMTEPDAKPGFDAIVPPESLWRNEFAARLMLR
                     FPFYRPGLKSQQLTMPLLVCICENDTTTPPASTIKAAERAPRGELRRYPYGHFDIYND
                     PKVKADQIDFLRRVVAGVTA"
     gene            1708..2037
                     /gene="hypA"
                     /locus_tag="VFC01_31305"
     CDS             1708..2037
                     /gene="hypA"
                     /locus_tag="VFC01_31305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005244680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HZN84141.1"
                     /translation="MHEMAITQSVVDAVCEHAAGRRVHSVKLEIGALCAVVPDSMQFC
                     FELATEGTVADGARLDLDMQPGAARCRTCAKEFELHDLILLCPCGSADVEVLAGRDLK
                     ILSMEVS"
     gene            2040..2828
                     /gene="hypB"
                     /locus_tag="VFC01_31310"
     CDS             2040..2828
                     /gene="hypB"
                     /locus_tag="VFC01_31310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HZN84142.1"
                     /translation="MCATCGCGDDSAVITLAGERHHDHPHDHSHEHTHVHTETISLEQ
                     KVLAKNDLIAEQNRAWLAERDILALNITSSPGAGKTTLLERTIGELASHRPVSVIEGD
                     QETLLDAERIRAAGARAVQVNTGAGCHLDAEMVRRALETLDPKPQSLLFIENVGNLVC
                     PALFDLGEHSKLVVTSVTEGADKPLKYPHMFAAAGLVVLNKIDLMPYVDFDLETFEKL
                     ARSINPAAAILPLSATTGEGTAAWHDWIELQANSRMSQRSVDKT"
     gene            2928..3983
                     /locus_tag="VFC01_31315"
     CDS             2928..3983
                     /locus_tag="VFC01_31315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012691718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HZN84143.1"
                     /translation="MPTEAAVKAEEALIHVLWINAGLSCDGDSVALTAATQPSIEEIA
                     LGALPGLPKIAVHWPLIDFECGPNGGADDFLAWFFKAERGELEPFVLVVEGSIPNEQI
                     KEEGYWCGFGNNPATGQPMTTSEWLDRLAPKATAVVAVGTCATYGGIHAMAGNPTGAM
                     GVPDYLGWDWKSKAGIPIVCVPGCPAHPDNMAETLTYLLYMATEQAPMIPLDEALRPQ
                     WLFGQTVHEGCDRAGYYEQGDFADEYGSPKCIVKLGCWGPVVKCNVPKRGWINGIGGC
                     PNVGGICIGCTMPGFPDKFMPFMDEPPGAHVSSTMSGLYGSVIRSLRHVTGRTVDKEP
                     RWRHPGTKLETGATRTW"
     gene            4072..5868
                     /locus_tag="VFC01_31320"
     CDS             4072..5868
                     /locus_tag="VFC01_31320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HZN84144.1"
                     /translation="MTTTIPEPSHVKRDPGQLVEMAWDPITRIVGSLGIYTKIDFDNR
                     EVVECHSTSSIFRGYSLFMKGKDPRDAHFITSRICGICGDNHCTCSVYAQNMAYGVRP
                     PHLGEWLINLGEAAEYMFDHNIFQENLVGVDYCEKMVSETNPSVLAKAENTQAPQADA
                     HGYRTIADIMRALNPFTGDFYREALQVSRWTREMFCLMEGRHVHPSTLYPGGIGTTAT
                     VQLMTDYMTRLMRYVEFMKKVVPMHDDLFDFFYEALPGYDQVGLRRTLLGCWGSFQDP
                     EHCNFAYKDMENWGRKMFVTPGVVVDGKLVTTSLLDINLGLRILLGSSYYDDWSDQEM
                     FVKTDPLGNPVDRRHPWNQHTNPHPQKRDMEDKYSWVMSPRWFDGKDHLALDTGGGPL
                     ARLWATALANLVDIGYVKSTGHSVQINFPKTALKGPVSLEWKIPQYGSNTIERNRART
                     YFQAYAAACALHFAEKALIEIRAGRTKTWEKFEVPDEGIGCGFTEAVRGVLSHHMVIR
                     DGKIANYHPYPPTPWNASPRDSYGTPGPYEDAVQGQPIFEENDREHFKGIDIMRTVRS
                     FDPCLPCGVHMYLGKGKTLDLLHSPTQSATGD"
     gene            5872..6762
                     /locus_tag="VFC01_31325"
     CDS             5872..6762
                     /locus_tag="VFC01_31325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HZN84145.1"
                     /translation="MAHRPDETQDDAQWRSAGDRIQTLLDASAAGGAAAHERAEQLVR
                     EVTDLYGAGLERMMQIAVAANPELAEAFTGDDLVASLLLVHGLHPHTVERRIADALDS
                     VRPYLGSHGGDVHLLEVDGDVVRLRFSGSCKSCPSSAVTLELAVEDAVRAAAPEIFSI
                     EVVAAEKDPSSGMIPAESLLTRLRSNGHRSTAWQPAPELGQLAPGEVGGFRVSGTVVL
                     ACRVGENVFAYQDRCANCTESLAGAQLDGAVLRCPRCGTGFDAVHAGASVDGTVHLDP
                     IPLLVRDGVLSMAVAETVAP"
     gene            6759..7391
                     /locus_tag="VFC01_31330"
     CDS             6759..7391
                     /locus_tag="VFC01_31330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HZN84146.1"
                     /translation="MTGSAYDVLARIRANRQAPEPEERCEMCSEPIADEHQHVVNVEG
                     RQLMCVCRGCYLLFTDTHAQLRYRAVPDRYLAFPDFALDRRRWEALQIPVGLAFFFRN
                     SALGRMVAFYPGPAGATESELDMEAWNDIRAADPRLDTLADDTEALLVRVPDDDTESP
                     DCHLLPIDACYEFVGRLRMLWRGFDGGGDVRAYMDDFFSAVGDRSKVVVR"
     gene            7388..8032
                     /locus_tag="VFC01_31335"
     CDS             7388..8032
                     /locus_tag="VFC01_31335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HZN84147.1"
                     /translation="MTEVTFAVLDVAPEPYAVTPILMARVGVAAVGDEPIHAIALRCQ
                     VRIDPLRRGYSDAEADGMIDLFGPRERWGSTQHTFLWQHATAMVPGFSGATQINLPLE
                     CTYDFEVASAKYLHALRDGMVPLQFLFSGTIFVQGPRGFSVQQVPWDREDRYDMPVSA
                     WRDLIQQHYPNAGWIRLHRDTIDALAAYRSRGALLSFDDAITSLLPATTTGDVR"
     gene            8029..9372
                     /locus_tag="VFC01_31340"
     CDS             8029..9372
                     /locus_tag="VFC01_31340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84148.1"
                     /translation="MTANWDGARAVADAVLYEGYLLYPYRANSRKNQSRWQFGVLGPR
                     GAEAAGIGENDSLSAQLLVQPDGAASVSGVVRFLQLQHRHAERALDDGRFEPVDELVA
                     GARSWLSWDEAVAREVPFGPSDLTKLPQQYAIPIAVGGGSDVEIVDGGRLVRTRRTIH
                     AELTIVAEHVGGLVRLTLSIRNSAAPAADKDDAIALSLIGTHLVAEVTDGEFVSLLEP
                     PASAAAAVANCHHHRCFPVLAGPPGEHDLMLVSPIILYDHPEIAEQSEGALYDSTEID
                     EILTLRVMTMTDEEKAQARATDPRAAEIIDRCDSMSPEAMLNLHGVLRNPHAETDGPG
                     LIPEIPEGVQWWDPLADTAVRPEIDAVLVNGVRVTRGSRVRLRPSRRADAQDLFFADR
                     IARVTSVHEDVDGDQHVGVVLEDDPAADLHDWYGRYLYFAPDEVEPLNERSASWK"
     gene            9510..9980
                     /locus_tag="VFC01_31345"
     CDS             9510..9980
                     /locus_tag="VFC01_31345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HZN84149.1"
                     /translation="MLVAGIGNIFLGDDGFGPEVMRHVPSRPADPRVRLMDYGIRGMH
                     LAYDLLDGCRALVLVDALPSRGAPGTVHVLEADHETLSSAIGLDAHAMDPAAVFASLR
                     ALGSTPPYTVVIGCEVDSVEERIGLSDAAAAAIPDAVRAIENVVAQLLTTERVG"
     gene            9984..10235
                     /locus_tag="VFC01_31350"
     CDS             9984..10235
                     /locus_tag="VFC01_31350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HZN84150.1"
                     /translation="MCLGIPGQVVRMLEGYGGQLALIDVAGEHRKVNVGMLPEETFAP
                     GDWVIIHMGFVVEKTDKAGADAAMAGLELMGRGGDEASP"
     gene            10232..12517
                     /gene="hypF"
                     /locus_tag="VFC01_31355"
     CDS             10232..12517
                     /gene="hypF"
                     /locus_tag="VFC01_31355"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HZN84151.1"
                     /translation="MTRCRLEARVAGVVQGVGFRPFVYTTAVTLGLAGSVRNDTSGAV
                     IEVEGDDRDIGDFLTRLADRPPPLAVIESIETRYIPVTGGTEFTISDSSRSDGARTLA
                     SPDVAMCAECAAEQRDPANRRYRHAFINCTNCGPRFTIIASLPYDRSSTAMAGFAMCV
                     DCAREYADPADRRFHAQPVCCPNCGPTVRYRDRDGRETEGGAGLQAARLLLRNGGVLA
                     VKGIGGYHLACDAADEQAVAELRRRKRRGDKPFAVMVPDLGVARVIAVVDDASARVLS
                     GPQRPIVLVPRLPGATVADAVAPHNPDLGVMTAYAPLHALLFGVAGDEPGPTALVMTS
                     GNLGGEPICFTDDEALDRLSRLADGWLMHNRDILVPCDDSVVRVVDGVELPIRRSRGY
                     APLPVALPVGLPPTLAVGADLKNTFAVAERKYAWLSQHIGDMDDLATLSAFGSAEHHL
                     EALTGVAPEVVVADSHPGYRSTAWAHRNAGDRPVRTVQHHHAHVAAVMAEHGLDGAKQ
                     VLGFAFDGTGYGPDGAVWGGEVLLADYKGYQRRAQLKYVPLAGGDVSVLRPYRMALSH
                     LWAAGLPWDDDLPPVRACPPRERKALAHQLTTGVGSVPTSSMGRLFDAVSALVGVRQV
                     VAYEAQAAIELEGISRDADGGTTRYEFDVDASAAPTVINPAPVLAAIVSDMRAGIRAT
                     VIGARFHRAVAELIVDLAGAEDPVPQTIALTGGVFQNALLLRLARNGLVAKGFDVITH
                     RHVPPNDGGIALGQLMVGNAG"
     gene            12522..13277
                     /locus_tag="VFC01_31360"
     CDS             12522..13277
                     /locus_tag="VFC01_31360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HZN84152.1"
                     /translation="MTAPSRQGLPTGHALFARYAFPPNELGYCGPADADVLLHGDDPA
                     EVAAHAKAFDGAWPYLRAIADAVGIADALDAEVVRSYWVGGDLLNRVDPTALLDRLRT
                     AFAGQVTGLLADLDRSVGVLAHHSFHVFVVYPWVRFLDRDPTTALQILQHCRIRWGTV
                     DSVDDEHLVMVSRPLTFLEDTLGLGHPTAERVRWSKSGASLITAPTPGDTVSAHWDWV
                     CSTLTDTEGDALADATCTTLDLVNSARERGRDK"
     gene            13274..14011
                     /locus_tag="VFC01_31365"
     CDS             13274..14011
                     /locus_tag="VFC01_31365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase assembly protein HupF"
                     /protein_id="HZN84153.1"
                     /translation="MTAIAQPGESFVDTELSADLAAAALDLARRFHDGATLWVISPQW
                     EPHAHHVAVEFVHPVIMGKRALPSVALVEPDPVAQARVASQPGDLLLAVASADEPAVV
                     DAMRRAPAWGALTLWIGSGPRPPAGAANHILWVDSDDPMVPATGRFVLMYHLLWELTH
                     VCFEHPGLLKSRGDDSTEDVCVTCSDEGRLGEVVLAPADATGQALVRTANGEEWVDVT
                     VLGDVAPNDLLLVHAGAAITRLSEVSR"
     gene            14008..14688
                     /locus_tag="VFC01_31370"
     CDS             14008..14688
                     /locus_tag="VFC01_31370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZN84154.1"
                     /translation="MTTHESESTSFLYPFIESEETDAASLLDDLASSARGKATESARL
                     QRESLDEYDEGLTAAGTGMAERFVRGGRLYTFGNGGSSTDAATLASLFSRPARGRPVA
                     AWSLAADNAVVTALGNDVGFELIFKRQIIAHARERDVAIALSTSGNSEDLMTAITEAK
                     QRGLLTIGFSGHDGGQMAAADDLDFCFTVHSQSIHRIQESHAMLGFRLWSVTQNHMAR
                     QPHRAEHS"
     gene            14685..15785
                     /gene="hypE"
                     /locus_tag="VFC01_31375"
     CDS             14685..15785
                     /gene="hypE"
                     /locus_tag="VFC01_31375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HZN84155.1"
                     /translation="MSTTEATTSAQREDRVLERIDKFRQRRPRLLDDVVTLAHGAGGK
                     SSAALVDAVFLEAFRNDELEQLGDAAVLRTASGEQLAFSTDSYVVSPRRFPGGSIGHV
                     AVHGTINDLAVSGARPQWLSAAFVIEEGFPIAELREIVADMSEAAAKAGVQIVTGDTK
                     VVGKGAADGVYISTAGVGVIPVGRQLSRDLVQPGDKVVLSGTIGEHGMAVMLARGDLA
                     IDADIASDTAPVHELVEILLAAAPSTRWMRDATRGGVGTVANELVKDSPFALILDEDK
                     LPVASQVLGACDMLGIDPLYVANEGKFVAIVPADEADAAIAALHDHPQGADAAVVGEI
                     MPEPHGIVALRTSFGGSRIVDMLVGDPLPRIC"
     gene            15799..16071
                     /locus_tag="VFC01_31380"
     CDS             15799..16071
                     /locus_tag="VFC01_31380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005244643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HZN84156.1"
                     /translation="MCLGIPGQVVDIVDSEQSLAKVDVNGVRRNISVRLLADDNLQVG
                     DWVLVHVGFAMAKIDEREAAMTLDQVQKMGENYQQEIDAFNESEIA"
     gene            16082..17209
                     /gene="hypD"
                     /locus_tag="VFC01_31385"
     CDS             16082..17209
                     /gene="hypD"
                     /locus_tag="VFC01_31385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HZN84157.1"
                     /translation="MKFVDEFRDPAAARALVKSITELAGTDEFKFMEVCGGHTHTIYR
                     HGIEHLLPESVELVHGPGCPVCVIPMGRVDDAMWLAEQPGVIFTTFGDMMRVPGSKGN
                     LIEAKARGADVRFVYSPLDALKVALDNPDRHVVFFAVGFETTAPSTAVTLVRARALGV
                     RNFSVFCNHVTIVPPIKAILESPDLRLNGFLGPGHVSTVVGLRPYRFVPQVYGKPMVV
                     AGFEPLDILASVHMLLQQIRDGRCEVENQYTRVVRPEGNTQALKLMAETFELRPHFEW
                     RGLGFISQSALKVHPDFAEYDAEIKFDMPGVRVADPKACQCGEVLKGVIKPWECKVFG
                     TACTPETPIGTCMVSPEGACAAYYNFGRLHRETAQLLGQRA"
     gene            17250..17582
                     /locus_tag="VFC01_31390"
     CDS             17250..17582
                     /locus_tag="VFC01_31390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84158.1"
                     /translation="MTTPGDHTSEEPQEERGIPGSRDTGSDKPSGGPTDRPSGTYEGD
                     ESVPDHGDEGGKPDFQTGTTNEPPGDAKPAAPPYEGRKEAADVGDDSAEEAGARTGGA
                     TRPSTDNR"
     gene            complement(17584..17775)
                     /locus_tag="VFC01_31395"
     CDS             complement(17584..17775)
                     /locus_tag="VFC01_31395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HZN84159.1"
                     /translation="MRLTEFNELVDGQFGPVRGASLLVDHVLTRFGRTAAQAIEDGVD
                     PRDVWRALCTDFDVPRDQW"
     gene            complement(17785..>18508)
                     /locus_tag="VFC01_31400"
     CDS             complement(17785..>18508)
                     /locus_tag="VFC01_31400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HZN84160.1"
                     /translation="MRALTARSWRQGLRQRSAARVEIGLPAVDPGPLRRLIATLPALE
                     VPRPDWPQEAVIVGPLHFEPTSSVLEIPGGSGPVVVVAPSTAMTGARGMAALALETLT
                     PGETLPEGARVVVSRLDGPEVTVPPWAVVGLGRQDELLAHADLVICGGGHGLVAKALL
                     AGVPMVVVPGGGDQWEIANRLVRQGSAQLVRPLTSESLVAAVSEVLSTPRYRDAAKRA
                     GASVAEVDDPVRVCHEALAASA"
BASE COUNT         3315 a         6151 c         6004 g         3038 t
ORIGIN      
        1 ctcatcagcc gcgcctggag acccacgtgg ctgtcgccca tctcgccctc gatctcggcg
       61 cggggcacca gcgcggccac cgagtcgatc acgatgacgt cgagcgcgcc cgaccggatg
      121 agcatgtcgg cgatctccaa cgcctgctcg ccggtgtcgg gctgggagac cagcaacgcg
      181 tcggtgtcga cgccgagcgc cttggcgtag tcggggtcga gcgcgtgctc ggcgtcgatg
      241 aacgcggcga tgccgccggc ggcctgtgcg ttggccaccg catgcagcgc gacggtggtc
      301 ttacccgagg attccaggcc atagatctcg accacgcggc cgcgcggcag cccgccgatg
      361 ccgagcgcca cgtccagggc gatcgatccg gtggggatga ccgagatcgg ctggcgcacc
      421 tcttcgccga gccgcatcac cgagcctttg ccgaagttct tgtcgatctg cgcgagggcc
      481 agctcgaggg ctttctcgcg gtcaggtgcc tgcgtcatgg gggtgggtct ccgtcctcgt
      541 caggtgttcg gtgatcgctt tcgatcagtt ggccgtgacg ctagaggccg ccaccgacaa
      601 gtcggcctga ccagcatcta gccgtcgaac actgcccaca gtagacgaac acgtgttcga
      661 ctcaaggaga cacgccgtac gaatgcaagc cgaccggaaa tgatcgcgcg atccccacaa
      721 accagcaggt caccggcgta ccttcggcca tatgaccgcg ccagaagaca tccgcattcc
      781 gtcccatggc gaacagctcg ccgcctacct ctaccgcccc gagtcgaccc aaggcgacgt
      841 gccctgcgtc gtcatggccc acggtttctc cgccacccgc gacgacgggc tgcccggcta
      901 cgccgaggcc ttccgcgacg ccggacttgc cgtcgtcgta tttgactacc ggcatttcgg
      961 ggcgtcaacc ggcgagccac gccagctgct cgacatcggc cgtcagcacg aggactaccg
     1021 cgcggtcatc acgtgggctc gcgagctcga cggaatcgac ccggatcgaa tcgtcttgtg
     1081 gggctcatcg ttcagcggcg gccacgtcct ggccgttgcg gcggccgatc ctcgcatcgc
     1141 agcggtgatc tcccaggcac ccttcaccga cgcgattccc acgctcatgc atgtgccact
     1201 gaagaatgcc gtccgtctca cggttgcggg gatacgggat cagttgggcg gctggcgcgg
     1261 ccgtgccctg cgtctcgcgc ctgccgtggg tgagccaggt acgttggcgg cgatgacgga
     1321 acccgacgcc aagcctggat tcgacgcaat tgttccgccg gagtcattgt ggcggaatga
     1381 attcgccgcg cgactcatgt tgcggttccc gttctaccgg cctggcctca agtctcagca
     1441 gctgacgatg ccgctactgg tctgcatatg tgagaacgac accacgacac cgcccgcatc
     1501 gacgatcaag gcggccgaac gggcgccacg cggtgaactc cgccgctatc cctacggtca
     1561 tttcgacatc tacaacgacc cgaaggtcaa ggccgatcag atcgacttcc ttcggcgggt
     1621 ggtcgccgga gtgaccgcgt aacaacgctg gaccgttcgg cgccgaccgt cctatcgtgg
     1681 ggttgagccg gcccgaaacg gggcaccatg cacgaaatgg cgataacgca aagtgttgtc
     1741 gacgcggtgt gtgagcacgc cgcaggtcgc cgagtgcaca gcgtcaagtt ggagatcggc
     1801 gcgctgtgcg ccgtcgtgcc cgactcgatg cagttctgct tcgagctcgc taccgagggc
     1861 accgtggccg acggagcgcg tctcgacctc gacatgcagc caggtgccgc ccgctgtcgc
     1921 acatgtgcca aagaattcga gctgcacgac ttgatcttgt tgtgcccctg cggcagtgcc
     1981 gatgtcgagg tgctcgccgg gcgagatctc aagattctct cgatggaagt gagctgacca
     2041 tgtgtgcaac gtgcggctgc ggcgacgatt cagcggtgat caccctcgcc ggcgagcgtc
     2101 atcacgacca tccgcacgat cactcgcacg agcacaccca cgtccatacc gaaacgatct
     2161 ccctggagca gaaggttctg gcgaagaacg acctcatcgc cgagcagaat cgcgcatggc
     2221 tggcggagcg cgacatcctt gcgttgaaca tcaccagctc gcccggcgcc ggcaagacga
     2281 ccctgctcga gcgaacgatc ggagagctgg caagccaccg gcccgtcagc gtgatcgaag
     2341 gcgatcaaga aaccctgctc gacgccgaac gcatccgggc cgccggtgcc cgcgcggtac
     2401 aggtcaacac aggcgcgggt tgccacctcg acgccgagat ggtccggcgt gcgctcgaga
     2461 cccttgatcc gaagccgcag tcgttgctgt tcatcgaaaa tgtcgggaat ctggtgtgcc
     2521 cggcgctgtt cgatctcggc gagcacagca aactcgtggt cacctccgtc accgagggcg
     2581 ccgacaagcc gctgaagtat ccccatatgt ttgctgcggc cggattggtc gtgctgaaca
     2641 agatcgattt aatgccttac gtagactttg atcttgaaac tttcgagaag cttgctcgat
     2701 caatcaatcc ggccgcggcg attttgccgc tgtccgctac gaccggtgag ggtacagcgg
     2761 cctggcatga ctggatcgaa cttcaggcaa acagccgtat gtcacaacga tccgttgaca
     2821 agacatagtc ccgggagtaa accgagaaat tagcgctgga gaagcgggcg ctacgtccga
     2881 gttgcgacgt agggagcccc agcccggctc ccggaaagct gtgacagatg ccaacagagg
     2941 cagcagtcaa agcagaagag gcattgatcc acgttctctg gatcaatgcc ggtttgagtt
     3001 gtgacggcga ttccgtggcg ctgactgccg ccacacaacc cagcatcgag gagatcgcgc
     3061 tcggcgcgct tcccggtctg cccaaaatcg ccgtgcattg gcccctcatc gacttcgagt
     3121 gcgggccgaa cgggggcgcc gacgatttcc tggcctggtt cttcaaggcc gagcgcggtg
     3181 aactcgagcc gtttgtgttg gtcgtcgaag ggtctatccc caacgagcag atcaaggaag
     3241 aagggtattg gtgcgggttc ggcaacaacc cggccaccgg acagccgatg acgaccagcg
     3301 aatggttgga ccggctggcg cccaaggcca cagcggtcgt cgcggtcggc acctgcgcca
     3361 cctacggcgg aatccacgcg atggcaggta atccgaccgg ggccatgggt gtgcccgact
     3421 atctggggtg ggactggaag agcaaggccg gtatcccgat cgtgtgcgtt cccggttgcc
     3481 cggcccatcc ggacaacatg gccgagacgc tcacctacct gctgtacatg gcgaccgagc
     3541 aggcaccgat gattccgctg gatgaggcgc tgcggccgca atggttgttc ggtcaaaccg
     3601 tacacgaggg gtgtgaccga gccggctact acgagcaagg cgatttcgcg gacgaatacg
     3661 gctcgcccaa atgcattgtg aaacttggct gttggggccc cgtcgtaaag tgcaatgtgc
     3721 cgaagcgcgg gtggatcaac gggatcggcg gctgcccgaa tgtcggcgga atctgcatcg
     3781 gctgcactat gcccggcttc ccagacaagt tcatgccgtt catggatgaa ccacccggcg
     3841 cccacgtctc ctcgacgatg tcgggactgt acggatcggt gatccgtagc ctgcggcatg
     3901 tcaccggacg gaccgtcgac aaggagccgc gctggcggca tccgggcacg aaactcgaaa
     3961 ccggagcgac tcgcacctgg taggtgcgga tactccggcg cgcacggcgg tccacgtctc
     4021 agacgtccaa tcacgctgtg gtgcagctga tcaacagaga gaagaagttc gatgacaacg
     4081 accattcccg agccgtcaca cgtgaagcgc gatcccgggc aactcgtgga gatggcgtgg
     4141 gatccgatca cccgcatcgt cggcagcctc ggcatctaca ccaagatcga cttcgacaac
     4201 cgcgaggtgg tcgaatgcca cagcacctcg tcgatcttcc gcggctactc gctgttcatg
     4261 aagggcaagg atccacgcga cgcacacttc atcaccagcc ggatctgcgg catctgcggt
     4321 gacaatcact gcacgtgctc ggtctacgca cagaacatgg catacggtgt gcggccacca
     4381 cacctcggtg agtggctgat caatctcggc gaagccgcgg aatacatgtt cgaccacaac
     4441 atcttccagg agaacctggt cggggtggac tactgcgaaa agatggtctc cgagaccaac
     4501 ccgagtgtcc tcgccaaggc ggagaacacc caggcaccgc aagcggacgc gcacggctac
     4561 cggaccatcg ccgacatcat gcgggcactc aacccgttta ccggcgactt ctatcgggag
     4621 gctctgcagg tcagccgatg gacacgagag atgttctgcc tcatggaggg caggcacgta
     4681 catccctcga cgctttatcc cggcggaatc ggcaccaccg caacggttca gctgatgacc
     4741 gactacatga cccggctgat gcgctacgtc gagttcatga agaaggtcgt gccgatgcac
     4801 gacgacctct tcgacttctt ctacgaggcg ctgcccggtt atgaccaagt gggtctgcga
     4861 cgcacgctgc tgggctgctg gggctcgttc caggatcccg agcactgcaa cttcgcctac
     4921 aaggacatgg agaactgggg ccgcaagatg ttcgtcacgc cgggcgtcgt cgtcgacggc
     4981 aagctcgtca cgacgtcgct gctggacatc aacctgggcc tccgaatcct tttgggcagc
     5041 tcgtattacg acgactggtc cgatcaggag atgttcgtca agaccgaccc gctgggcaat
     5101 ccggttgatc ggcgccaccc gtggaaccag cacaccaacc cccacccgca gaaacgggac
     5161 atggaggaca agtacagctg ggtgatgtca ccgcggtggt tcgacggcaa ggatcacctg
     5221 gcactggaca ccggcggtgg cccgctggcg cggctgtggg caacggcgct ggccaacctc
     5281 gtcgacatcg gctacgtgaa gtccacgggt cacagcgtgc agatcaactt cccgaagacc
     5341 gccctcaagg ggccggtttc gctcgagtgg aagatcccgc agtacggcag caacacgatc
     5401 gagcgcaacc gcgcccgaac gtatttccag gcctatgccg cagcctgcgc gctgcatttc
     5461 gcggagaaag cgctgatcga aatccgtgcc ggccgaacca agacgtggga gaagttcgag
     5521 gtgcccgacg agggcatcgg ctgcggattc acagaagcgg tgcgcggggt gctgagccac
     5581 cacatggtga tccgcgacgg caagatcgcg aactaccacc cctatccccc aacgccgtgg
     5641 aacgccagcc cgcgcgacag ctacggcaca ccggggccat acgaggacgc ggtccagggc
     5701 caaccgatct tcgaggagaa cgacagggag cacttcaagg gcatcgacat catgcgcacg
     5761 gtacgcagtt tcgacccgtg cctgccgtgc ggtgtgcaca tgtatctggg caaggggaag
     5821 accctggacc tgctccactc ccccacccaa tccgcgaccg gggattagcg gatggcccat
     5881 cgcccggacg aaacccagga tgacgcgcag tggcgctcgg cgggcgatcg gatacagaca
     5941 ttgctcgacg ccagcgccgc tggtggagca gccgcgcacg aacgtgccga gcagcttgtc
     6001 cgtgaggtaa ccgacctcta cggcgcaggc ctcgagcgaa tgatgcagat cgccgtcgcc
     6061 gccaatcccg aactcgcaga ggcgtttacc ggtgacgacc tggtggccag cctgctgctc
     6121 gtgcacgggc tgcacccgca caccgtggaa cggcgcatcg ccgacgcact cgacagcgtg
     6181 cggccgtacc tgggatcaca cggcggcgat gtccacttgc tcgaggtcga tggcgatgtc
     6241 gtgcggctgc ggttctcggg cagttgcaag agctgcccgt cgtccgcggt gacgctggaa
     6301 ctggcggtcg aggatgcggt gcgcgccgcc gcgcctgaga tcttctcgat cgaagtggtt
     6361 gccgcggaaa aggacccgag ctccgggatg attccggcag agtcactgct gacccggttg
     6421 cgcagcaacg ggcatcggtc gacagcgtgg caaccggccc cggagctcgg ccagctcgcc
     6481 ccaggagaag tcggcggctt ccgcgtatcg ggaacggtcg tgctggcgtg ccgggtcggt
     6541 gagaacgtct tcgcctacca ggaccggtgc gccaactgca cggaatcctt ggccggggcc
     6601 caactcgatg gtgcggtgtt gcggtgtcca cggtgcggca ccggcttcga cgcggtgcac
     6661 gccggcgcga gcgtcgatgg cacagtccat ttggacccca taccgctgtt ggtgcgcgac
     6721 ggtgtgctgt cgatggcggt cgctgaaacg gttgcgccat gaccggcagc gcatacgacg
     6781 tactggctcg catccgcgca aaccggcaag cacccgagcc cgaggagcgg tgcgagatgt
     6841 gttcggagcc catcgccgac gagcaccagc acgtggtgaa tgtcgaaggc agacagctga
     6901 tgtgcgtgtg ccgcggctgc tacctgttgt tcaccgacac tcatgcccag ttgcggtacc
     6961 gtgcagtgcc cgatcgctac cttgcctttc cggacttcgc gctggaccgc cgcaggtggg
     7021 aggcgctgca gatcccggtg gggttggcgt tcttcttccg caactcggca ctcggccgca
     7081 tggtggcgtt ctatcccggg ccggccggtg caacggagtc cgaactcgac atggaggcct
     7141 ggaacgacat ccgcgccgcc gaccctcggc tggacacgtt ggccgacgac accgaggcgc
     7201 tgctggtccg ggtgcctgac gacgacaccg agtcgccgga ttgccacctg ctgccgatcg
     7261 acgcctgcta cgaattcgtc gggcggttgc ggatgttgtg gcgcggcttc gacggcggcg
     7321 gggacgtgcg cgcctacatg gacgacttct tcagcgctgt cggcgatcgc agcaaggtgg
     7381 tcgtccgatg accgaggtga cattcgcagt cctcgacgtg gcgcccgaac cctacgcggt
     7441 caccccgata ctcatggccc gggtgggcgt tgccgccgtc ggggacgagc cgatacacgc
     7501 gatcgcgttg cgttgtcagg ttcgcatcga cccgctgcgt cgcgggtatt ccgacgccga
     7561 agccgacgga atgatcgatc tgttcggacc gcgtgaacgc tggggctcca cccagcacac
     7621 gttcttgtgg cagcacgcga ccgccatggt gcccggcttc agcggggcca cccagatcaa
     7681 cctcccgctc gaatgcacct acgacttcga ggtggcctcg gccaagtacc tgcacgccct
     7741 gcgcgacgga atggttccgc tgcagttcct cttcagcgga acgattttcg tgcagggccc
     7801 gcgcggattc tcggtccagc aggtgccgtg ggaccgggag gaccgctacg acatgccggt
     7861 gtcggcatgg cgcgatttga ttcagcagca ctaccccaac gccggatgga tacggcttca
     7921 tcgtgacacc atcgatgcgc tggcggccta ccgttcgcgg ggggccctgt tgtcgttcga
     7981 cgatgcgatc acctcgctgc tcccggcgac cacgaccgga gacgtccgat gaccgcgaat
     8041 tgggacgggg cccgcgccgt cgccgacgct gtgctgtacg agggctacct tctgtacccc
     8101 tatcgggcta actcgcgaaa gaaccagtcc cgctggcagt tcggggtact cgggccgcgc
     8161 ggagccgagg ccgccgggat tggtgagaac gacagccttt cggcgcagct gcttgtgcag
     8221 cccgacggcg ccgcgtcggt gtcgggcgtt gtgcgtttcc tgcagttgca gcatcgccac
     8281 gcggaacgcg ccctcgatga cggccgattc gagccggtcg acgaactggt cgcgggtgcg
     8341 cgttcgtggc tgagttggga cgaggccgtg gcacgggaag tcccattcgg gccgtccgat
     8401 ctcaccaaac tcccgcagca gtacgcaatt ccgatcgccg tcggtggcgg cagcgatgtc
     8461 gagatcgtcg atggcggtag actggttcgc acccggcgca cgattcacgc cgagctcacc
     8521 atcgtcgccg aacatgtcgg ggggcttgtt cgcctcaccc tctcgatacg caacagcgct
     8581 gcgcccgcgg cggacaagga cgacgcgatc gccctgtcgt tgatcggcac acacctggtt
     8641 gccgaggtca ccgacgggga attcgtgtca ctgctcgaac cccccgcgtc ggccgccgcc
     8701 gcggtagcca actgccatca ccaccggtgc ttccctgtgc tcgccggtcc gccaggcgaa
     8761 cacgatctga tgctggtgtc gccgatcatc ctctacgacc atcccgagat cgccgaacaa
     8821 agcgaaggcg cgctgtacga ctcgacggag atcgacgaga tcctcaccct gcgggtcatg
     8881 acgatgaccg acgaagaaaa agctcaggct cgggccaccg atccgcgcgc cgcagagatc
     8941 atcgaccggt gcgactcgat gtcgccggag gcgatgctga atctgcacgg ggtgctgcgc
     9001 aacccgcacg cggagaccga cggccccgga ttgatacctg aaatccccga gggcgtccag
     9061 tggtgggatc ccttggccga caccgcggtc cgccccgaga tcgacgctgt gctggtcaac
     9121 ggcgtccggg tgacgcgggg tagccgtgtg cggttacggc cgtcacggcg ggccgacgcc
     9181 caggatctgt tcttcgcgga caggatcgca agggtcactt ccgtacacga ggacgtggac
     9241 ggcgaccagc atgtcggcgt cgtactggag gacgaccccg ccgccgacct gcacgactgg
     9301 tacggccgct atctgtactt cgcaccggac gaagtggaac cactcaatga aaggagtgcg
     9361 tcatggaagt agtgggttgg gtcgccgttg gcatcgccgc ggttgtggtg atcgccggag
     9421 gttacctcgg actccgttcc ataccggatg cccgccggta catgaagatt cgccgcatgt
     9481 agccaaggta ggtacaaacc acgcccgaca ttctggtagc cggcatcggc aacatcttcc
     9541 tcggtgacga cgggttcggc cccgaggtga tgcgccacgt cccgtcgcgc cccgccgacc
     9601 cgcgagtccg gctgatggac tacggaatcc gaggcatgca cctggcctac gacctgctcg
     9661 acggctgccg cgcgctggta ctcgtcgacg ccctgccgag ccggggagca ccgggcaccg
     9721 tccacgtatt agaagcggac cacgaaacgc tgtcgtccgc aattggactc gatgctcacg
     9781 cgatggatcc cgccgcagtg tttgcaagcc tgcgcgccct gggcagcacc ccgccataca
     9841 ccgtcgtcat cggctgcgag gtcgatagcg tcgaagagag aatcgggctg tccgacgccg
     9901 ccgccgccgc catccccgac gcggtgcgcg ccatcgaaaa tgtagtggcg caactactca
     9961 ccaccgagcg cgtgggctga accatgtgtt tgggcatccc agggcaggtg gtgagaatgc
    10021 tggagggcta tggcggccag ctcgccctca ttgacgtcgc cggcgagcac cgcaaggtca
    10081 acgtcggcat gctgcccgaa gagacgttcg cacccggcga ctgggtgatc atccacatgg
    10141 gtttcgtcgt cgaaaagacc gacaaggccg gagccgacgc cgcaatggcc ggcctcgaac
    10201 tcatgggccg cggcggggat gaggcctcgc catgacccgg tgccgccttg aggctcgcgt
    10261 ggccggcgtc gttcagggcg tcggcttccg gcccttcgtc tacaccacgg cggtgaccct
    10321 ggggttggcc ggatccgtgc gcaacgacac gtcgggtgcg gtcatcgagg tcgagggtga
    10381 cgaccgcgac atcggcgact tcctgacccg gctcgcagac cgaccgccac ccctggctgt
    10441 catcgaatcc attgaaacgc ggtatatccc ggttaccggc ggtaccgaat tcaccatctc
    10501 ggattcctca cgatccgacg gcgcccgcac actggcctcc cccgacgtcg cgatgtgcgc
    10561 cgaatgcgcc gcagaacaac gcgaccctgc caaccggcgc tatcggcacg cgttcatcaa
    10621 ctgcaccaac tgcggaccgc ggttcacgat catcgcctcg ctgccctacg accggtcctc
    10681 gaccgcgatg gcggggttcg caatgtgtgt cgactgcgcc cgcgagtatg ccgatcccgc
    10741 cgaccgacga ttccacgccc agcccgtgtg ctgcccgaac tgcggaccta ccgtgcgcta
    10801 ccgggaccgc gacggccgcg aaaccgaagg cggtgcggga ctccaggcag cccgactgct
    10861 actgcggaac ggcggcgtac ttgcggtcaa gggcatcggc ggctaccacc tcgcctgcga
    10921 cgccgcggac gaacaagccg tggcggaact tcggcgacgt aagcgccgcg gcgacaagcc
    10981 attcgccgtc atggtgcccg atctgggagt ggcccgcgtc atcgcggtcg tcgacgacgc
    11041 gtctgcgcgc gtgctgtcgg gcccgcagcg cccgatcgtt ctggtacccc gactccccgg
    11101 cgccacggtc gccgatgcgg tggccccgca caaccccgac ctcggcgtca tgacggcata
    11161 cgcgccgctg cacgcgcttc tgttcggtgt ggccggtgac gagccgggtc cgacggcgct
    11221 ggtgatgacc tcgggcaacc ttggcgggga acccatctgt ttcaccgacg acgaagcgct
    11281 cgaccggctt tcgcgcctgg ccgatgggtg gctgatgcac aaccgggaca tcctggtgcc
    11341 gtgtgacgac tccgtcgtgc gagtggtcga cggtgtcgag cttccgatcc ggcgctcgcg
    11401 cggttacgct cctcttcccg tcgcgctgcc cgtggggctg ccgccgaccc tggcggtggg
    11461 agcagacctc aagaacacgt tcgccgtcgc cgaacgcaag tacgcgtggc tcagccagca
    11521 catcggcgac atggacgacc tggccaccct gtcggcgttc gggtctgcgg agcatcatct
    11581 ggaggcgttg accggcgtcg caccggaagt cgttgtcgcc gactcccatc cgggctatcg
    11641 atcgaccgcg tgggcgcacc gaaacgcggg cgaccggccg gtgcggacgg tacagcacca
    11701 ccacgcgcac gtcgcggcgg tgatggccga gcacggcctc gacggcgcca agcaggtact
    11761 cggtttcgcg ttcgatggca ccggctacgg acccgacggc gcggtctggg gcggtgaggt
    11821 gctgctcgcg gactacaagg gctaccagcg gcgggcgcag ctgaaatatg tgccgctggc
    11881 cggaggcgac gtcagcgtgc tacgccccta ccggatggcg ctgtcgcacc tatgggcagc
    11941 cgggctgccc tgggacgacg atctgccccc tgtgcgagca tgcccgccgc gcgagcgaaa
    12001 ggcactggcg catcagctga ccaccggggt gggcagtgtt ccgacatcga gcatgggcag
    12061 gctcttcgat gcggtgtcgg cgctggtcgg tgtccggcaa gtcgtcgcct atgaggccca
    12121 ggccgcaatc gaattggagg gaatttcgcg cgacgccgac ggaggcacga ctcgttatga
    12181 gttcgatgtc gatgcgtcag cggcgccaac ggtgatcaac ccggctcccg tgctggccgc
    12241 gatcgtcagc gacatgcgtg ccggcatacg ggcaactgtg atcggcgcgc gtttccaccg
    12301 cgcggttgcc gagctgatcg tcgatctcgc aggcgcggag gatccagtcc cccagaccat
    12361 cgcactgacc ggtggcgtgt tccagaacgc gctgctgctg cgactggcac gaaacggcct
    12421 tgtggccaag ggctttgacg tgattactca ccgtcatgtt ccgccgaacg acggcggcat
    12481 cgcgctgggt caactcatgg tcggaaacgc ggggtgatca gatgaccgca ccgtctcgac
    12541 aaggccttcc caccggtcat gcgttgttcg cgcgttatgc gttcccgccc aacgagctgg
    12601 gctactgcgg gcccgccgat gcggacgtgc tgctccacgg agacgatcca gcagaagtcg
    12661 ccgcgcacgc caaagcgttc gacggcgcgt ggccgtatct gcgggccatc gccgacgcgg
    12721 tgggcattgc cgatgcgctc gacgccgaag tggtgaggag ctactgggtc ggcggagacc
    12781 tgctgaaccg cgtcgacccc accgcgctgc tggatcggct tcgcaccgca ttcgcgggtc
    12841 aggtcaccgg ccttctcgcc gacctcgatc gatcagtcgg tgtgctggcc caccacagct
    12901 tccatgtgtt cgtggtttac ccatgggtgc ggttcctcga ccgcgacccc accacggcac
    12961 tgcagatact gcagcactgc cgaatacgtt ggggtacagt cgactccgtc gacgatgagc
    13021 acttggtgat ggtgtcgcgc ccgctcacgt tcctggagga caccctcggc ctcggccacc
    13081 caaccgccga gcgggtgcgg tggagcaaga gcggcgcgtc gctgatcacc gcgccgacgc
    13141 caggcgacac cgtgtccgcg cactgggatt gggtctgctc caccctcacc gacaccgaag
    13201 gtgacgcgct agccgacgcc acgtgcacca cgctggacct cgtgaactct gcccgcgaaa
    13261 gggggcgaga caaatgaccg caattgcaca accgggtgaa tcctttgtcg acacagagct
    13321 atccgcggat ctggcggccg cagcgctgga cctggcccgc cgattccacg acggcgccac
    13381 cctctgggtg atctcacccc agtgggaacc gcacgcacac cacgtcgcgg tcgagttcgt
    13441 tcacccggtc atcatgggca agcgggcgct gccgtccgtt gcgctggtcg aaccggatcc
    13501 ggtggcgcag gcacgcgtgg ccagccaacc cggcgatctg cttctggcgg tggcatcggc
    13561 cgatgagccc gctgtggtgg acgccatgcg acgagcgccg gcgtggggcg ccctgacgct
    13621 gtggatcggc tccgggccgc ggcccccggc cggcgccgcg aaccacatcc tctgggtcga
    13681 ctcggacgac cctatggtgc cggccaccgg gcggttcgtg ttgatgtacc acctgctttg
    13741 ggaactgacc cacgtctgct tcgaacaccc tgggctgctg aaatcccgcg gtgacgactc
    13801 cactgaggac gtctgcgtca cctgtagtga cgagggacgg ctcggcgaag tagtactggc
    13861 acccgccgac gcgaccggcc aagcgctcgt ccgcaccgcc aacggtgagg aatgggtcga
    13921 cgtcaccgtc cttggtgacg tcgcacccaa cgatctcctt ctggtgcacg cgggagcggc
    13981 gatcacacga ctgagcgagg tgagccgatg accacacacg aatcagagag caccagcttc
    14041 ctgtacccat tcatcgaatc ggaggagacc gacgcggcaa gcctgctgga cgacctcgcc
    14101 tcttcggcgc gcggcaaggc cacggaaagc gcccgcctgc aacgtgagtc gctcgacgag
    14161 tacgacgagg ggttgaccgc cgcgggaacc ggcatggccg agcggttcgt gcgcggcggc
    14221 aggctctaca cgttcggcaa cggcggcagc tccaccgacg ccgcgacgct cgcgtcgctg
    14281 ttcagccgcc ccgcacgggg gcggcccgtc gcggcgtggt cgctggcggc cgacaacgcg
    14341 gtggtgaccg cgctaggtaa cgacgtcggg ttcgagctga tcttcaagcg acagatcatc
    14401 gctcacgcgc gcgagcgcga tgtcgcgatc gccttgtcga catcaggcaa ctccgaggac
    14461 ctgatgacgg cgatcaccga agccaagcag cggggcctgt tgacgattgg cttttccggc
    14521 cacgacggcg gccagatggc cgcggccgac gatctcgact tctgcttcac cgtgcacagc
    14581 caaagcatcc accgcatcca ggagagccac gccatgctcg gcttccggct gtggtcggtg
    14641 acccaaaacc acatggcacg tcaacctcac agggcggagc attcatgagc accacggaag
    14701 cgacgacctc cgcccagcgc gaggaccgtg tgctggaacg gatcgacaag ttccggcagc
    14761 gccggccacg gctgctggac gacgtggtga cgctcgcgca cggggcgggc ggcaagtcgt
    14821 ccgcggcgct ggtcgacgcg gttttcctcg aggcgttccg caacgacgaa ctcgaacaac
    14881 tcggcgacgc agccgtcctg cgaaccgcat cgggcgaaca actcgcattc tcgaccgact
    14941 cgtatgtcgt ttcgccgcgg cggtttccag gcggatccat cggccacgtc gccgtgcatg
    15001 gcacgatcaa cgatctcgcg gtgtcgggtg cgcgtccgca atggctgtcg gcggcgttcg
    15061 tcatcgaaga gggtttcccg atcgccgaac tccgcgaaat cgtcgcggat atgagcgaag
    15121 ccgccgcgaa ggcgggcgtg cagatcgtca caggcgacac caaggtcgtc ggcaagggcg
    15181 ccgccgacgg tgtctacatc tccaccgcag gcgtcggcgt gatcccggta ggcaggcaac
    15241 tgtcgcgcga cctcgtccaa ccgggcgaca aggtagtgct gtccgggacg atcggcgagc
    15301 acgggatggc cgtcatgctg gcccgcggcg accttgccat cgatgccgac atcgcctccg
    15361 acaccgcgcc ggtgcacgag ttggtcgaaa tactgcttgc ggcagcgcca tcgacgcgat
    15421 ggatgcgcga cgctacgcgt ggtggcgttg gcaccgtggc caacgaactg gtcaaggact
    15481 ccccgttcgc gctcatactg gacgaggaca agctgccggt ggcgtcacag gtgcttggag
    15541 cctgcgacat gctcggcatc gacccgctgt acgtggcgaa cgagggcaaa ttcgttgcca
    15601 tcgttccggc cgacgaggcc gacgcggcga ttgccgcatt gcacgaccat ccgcaagggg
    15661 ccgatgccgc ggtggttggc gagatcatgc cagaaccgca cggaatcgtt gcgctccgaa
    15721 cgtcgttcgg cggcagccgg atcgtcgaca tgctcgtcgg tgacccgcta ccgcgcatct
    15781 gctgaaagga gattgaccat gtgtcttggg ataccgggcc aagtggtcga catcgtcgac
    15841 tccgaacagt ccctggccaa ggtcgacgtc aacggcgtcc gaagaaacat cagcgtgcgc
    15901 ctgctggccg acgacaacct gcaggtcggt gactgggtgc tcgtgcacgt cggctttgcg
    15961 atggcgaaga tcgacgagcg cgaggccgcg atgacgctcg accaagtgca gaagatgggc
    16021 gagaactacc agcaggaaat cgacgcgttc aacgagtccg aaatcgcctg agaggcttga
    16081 catgaagttc gtagacgaat tccgcgaccc ggcggcagca cgtgcgttgg tcaagtccat
    16141 cacagaattg gcaggcacgg acgagttcaa gttcatggag gtgtgcggcg gtcacacgca
    16201 caccatctac cggcacggca tcgagcacct gctacccgaa tccgttgagc tggtgcatgg
    16261 accggggtgt ccggtgtgcg tgattccaat gggccgtgtc gacgacgcga tgtggctggc
    16321 cgagcagccc ggcgtcatct tcaccacgtt cggcgacatg atgcgggtgc ccggatcgaa
    16381 gggcaacctc atcgaggcga aggcccgagg cgccgacgtg cgcttcgtgt actctccgct
    16441 cgatgcgctc aaggtggcgc tcgacaaccc cgaccgccat gtggtgttct tcgcggtcgg
    16501 attcgagacg acggcgccgt ccaccgcggt caccctcgtt cgggcgcgcg cgctcggtgt
    16561 ccgcaacttc agcgtgttct gcaatcacgt caccatcgtt ccgccgatca aggccatcct
    16621 cgagtcaccc gatctgcggc tcaacgggtt cctcggacct ggccacgtat ccaccgtggt
    16681 gggcctgcgg ccgtaccggt tcgtgccgca ggtctacgga aaacccatgg tggtggcggg
    16741 gtttgagccg ctcgacatcc tcgcgtcagt gcacatgctg ctgcagcaga ttcgcgacgg
    16801 gcgctgcgag gtcgaaaacc agtacacgcg cgtggtacgc ccggagggca acacacaggc
    16861 gctgaagctg atggccgaga cgttcgagct gcgcccccat ttcgagtggc ggggactggg
    16921 tttcatctcg cagagtgccc tgaaggttca tcccgacttc gccgagtacg acgccgagat
    16981 caagttcgac atgccgggag tgcgcgtcgc cgaccccaag gcatgccagt gtggcgaagt
    17041 tctcaagggc gtgatcaaac cgtgggagtg caaggtgttc ggcacggcgt gcaccccgga
    17101 gacgccgatc ggcacatgca tggtgtcccc cgaaggtgcc tgtgcggcct actacaactt
    17161 cggccggcta caccgagaga cggcccagct gctgggtcag cgtgcctaac aactagcgtc
    17221 gccgcaacgc gcggcggaga ggagatgtca tgacgacccc gggcgaccac acatctgaag
    17281 aaccacaaga agaacgggga attcctggat cgcgggacac cggatccgac aagccgtcgg
    17341 gcgggcccac ggatcggcca tcgggcactt acgagggcga cgagtccgtg ccagatcacg
    17401 gcgacgaggg cggcaaaccg gacttccaaa cggggacgac gaacgaaccg ccaggcgatg
    17461 ccaagcccgc cgccccgccg tacgaaggcc gcaaagaggc cgctgacgtc ggcgacgaca
    17521 gcgccgagga ggccggagca aggaccggtg gagccacccg cccctccacc gacaatcgct
    17581 gaatcaccac tgatcgcgcg gcacatcgaa gtcggtgcac aacgcccgcc acacgtcgcg
    17641 cgggtcgacg ccgtcctcga tcgcctgcgc ggcggtgcgc ccgaatctgg tcagtacatg
    17701 gtcgacgagc agggaagcgc cgcgcaccgg tccgaactgt ccgtcgacca gttcattgaa
    17761 ttccgtcaac cgcacgtcgc caacctacgc cgacgccgcc agcgcttcat ggcacacccg
    17821 taccggatcg tcgacctcgg cgacgctggc gccggctcgt ttcgccgcgt ccctgtaccg
    17881 cggcgtcgac aacacctcac tgacggccgc caccagcgat tccgacgtca atggccgcac
    17941 cagttgcgca ctaccttggc gcacaagccg attcgcgatc tcccactgat cgccgccgcc
    18001 gggcaccacg accatcggca ccccggccag cagcgccttg gccaccaagc cgtgtccacc
    18061 accacagatg accagatcgg cgtgagcaag cagttcgtcc tgccggccga ggccgaccac
    18121 tgcccacggc ggcaccgtaa cctcaggtcc gtcgagtcgc gacaccacca cgcgcgcacc
    18181 ttcgggcagc gtctcccccg gcgtcagcgt ctctagcgcc agagcggcca tcccccgggc
    18241 gcccgtcatc gccgtcgacg gcgccaccac cacaacggga ccggaccccc ctgggatctc
    18301 cagcacggac gacgtcggct cgaagtgcaa cgggcccacg atcactgcct cttgcggcca
    18361 atccgggcgc ggcacctcca gagccggcag ggtggcgatg agacgccgca acggtccggg
    18421 atcaactgcg ggcaaaccga tttcgactct ggccgccgac cgctgccgca ggccctggcg
    18481 ccaggatcgt gcggtgagcg ctcgcatg
//
LOCUS       DASRJA010000697         5452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1787254,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000697 DASRJA010000000
VERSION     DASRJA010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787254"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1980
                     /locus_tag="VFC01_31405"
     CDS             346..1980
                     /locus_tag="VFC01_31405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN84161.1"
                     /translation="MTRSMLGVASGMMPDSSWARFGLNLARRPGVVTKQVELFGRELA
                     SIAAGTSDRAPAKPDKRFSDPAWQSNPLTKRSMQAYLAATELVDSLFADAELDWRDAE
                     RIRFMLDVIMEGLAPSNSPLISPLGWKALIDTGGLNVVRGMRRFVRDMAVPPRIPSMV
                     EPDAFTVGEDLAVTPGAVVYRNEVFELIQYTPQTEKVWAVPLLMVPPVINKFYILDIA
                     PGRSMVEYLLRQGHQVFAISWRNPTAAQRSWGCDTYGAAILDALEAIQRVTGSQSAHV
                     QASCSGGILAAMTAAHLSATGEAERIASLTLMVTVLDQGREGLAAAVDETAAKLAIAF
                     SARKGFLDGRALAEVFAWLRPTDLVWRYWVNNYLQGRSPAPFDVLFWNSDTTRMPAAL
                     HRDMLLMGQHNALAEPGAVRMLGTPVDLGTVTADTYVVAGIADHISPWQACYRSARML
                     GSKDLRFVLSSSGHIAALVNPPGNPKASYRAGNVDERDPTRWAASAETHSDSWWPDFS
                     AWLAQRGGEQKPAPTALGGDGLAALGPAPGSYVHVR"
     gene            2178..2996
                     /gene="phaZ"
                     /locus_tag="VFC01_31410"
     CDS             2178..2996
                     /gene="phaZ"
                     /locus_tag="VFC01_31410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006112 - energy reserve metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxyalkanoate) depolymerase"
                     /protein_id="HZN84162.1"
                     /translation="MNDENLVAVLGQQIRVDIRWGTGVPLVLCNGIGASLEVLDPFVA
                     QLDPDTTVVRFDVPGSGASPDSPFPYRFPYLAAVLGTLLKKIGIIDRRAGISQVDVLG
                     LSWGGGLAQQFAFQNPRRCRRLILVSTGTGSLMIPGKPPVLAKMLTARRFLDHRYAAS
                     IAPEIYGGTARSDPMMIKRVFDRQLMAGSRVGYLHQLLAGAGWTSVFMLPLVRQPTLI
                     VAGSDDPIIPLVNARIMNRLLPNSALHIHDGGHVELITNAAEQARVIEEFRRKA"
     gene            3394..4560
                     /locus_tag="VFC01_31415"
     CDS             3394..4560
                     /locus_tag="VFC01_31415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN84163.1"
                     /translation="MQRFGDPSFDLFKLSDEHNELRAVLRDLCEKEIAPYAADVDESS
                     RFPEEALKVLNDTGFSAVHIPEEYGGQGADAVAGCIVVEEVARVCASSSLIPGVNKLG
                     TIGLILSGSDELKKQVLPTLASGDAMASYALSEREAGSDPASMRTRARADGDDWVLNG
                     SKCWITNGGKSTWYTVMAVTDPEKGANGISAFVVHEDDEGFTVGPKERKLGIKGSPTT
                     ELYFENCRIPGDRIIGEPGTGFKTALASLDHTRPTIGAQAVGIAQGALHAAIAYTKER
                     KQFRRPVSDFQGVQFMLADMAMKVEAARLMVYSAAARAERGEAALSFISSAAKCFASD
                     VAMEVTTNAVQLFGGAGYTVDFPIERMMRDAKITQIYEGTNQIQRVVMARALLA"
     gene            4701..>5452
                     /locus_tag="VFC01_31420"
     CDS             4701..>5452
                     /locus_tag="VFC01_31420"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN84164.1"
                     /translation="MADADPQATQRDVLTGIPAELSISGFDDVVEIGHGGFGVVYRCV
                     QPLLDRDVAIKVLTGDLDPDNLDRFLREQRAMGRLSGHPHIVTIFQVGTTAGGRPYIV
                     MPCHRKGSLEALIRQHGPMDWCETLRIGVKLAGALEAAHRAGILHRDVKPGNVLLTEY
                     GEPQLTDFGIARIAGGFQTGTGVIIGSPAFTAPEVLAGAAPTSASDVYSLGATLFCIL
                     TGHAAFERRSGEQVVAQFLRITSQPIPDLREQG"
BASE COUNT          971 a         1712 c         1793 g          976 t
ORIGIN      
        1 caaataccgg gtcgggcggg ccgtcgcccg gcggggacgc gcgatcggcg ccgatcggct
       61 tgacgtggag ttggccctgc tgctgtgccg ctggtacggc agtgcggtta ctttgcagcg
      121 ccccggatag caaccgccag cgggttagtt atcgccgcgc cacaacctac ccgcgggtat
      181 tggtcccacg gggacgaagg caatgctgcc aagcacccct aacctcagcc ttgcgcacgg
      241 caccgacgcc gacgaagtga aatccaaagc aaccgaaggc gatagtcgat gccaagccca
      301 gacgttcagc acccggctga aattgctaca ccacatgatt tgctgatgac ccggtcgatg
      361 ctcggcgtcg caagcgggat gatgccagac agctcgtggg ccaggttcgg cttgaatctg
      421 gcgcgccgcc cgggcgtcgt caccaaacag gtggagttgt tcgggcgcga gctcgcctcg
      481 atcgcggcgg gcacatccga tcgcgctccg gccaagcccg acaagcgatt cagcgatcca
      541 gcatggcaga gcaatccgtt gacgaagcgt tcgatgcagg cgtacttggc cgccaccgag
      601 ctcgtggaca gcctgttcgc cgacgcggaa ctcgattggc gcgacgctga acgaatccga
      661 ttcatgctcg acgtcatcat ggaagggctc gcaccgagca acagcccatt gatcagcccg
      721 ttgggctgga aggcgctcat cgacacaggc ggtctcaacg tcgttcgagg gatgcggcgc
      781 ttcgtgcgcg atatggctgt gccgccgcgg attccgtcga tggtggagcc cgacgcattc
      841 acggtcggag aggatctcgc cgtcacaccg ggagcggtcg tgtaccgcaa cgaggtgttc
      901 gaactcatcc agtacacacc tcaaaccgag aaggtgtggg cggtgccgct gctgatggtg
      961 ccgccggtga tcaacaagtt ctacatcttg gacatcgccc ccggtcggag catggtggag
     1021 tacctcctgc gccagggtca ccaggtcttc gccatctcgt ggcgcaatcc gactgcggcg
     1081 caacggtctt ggggctgcga tacgtacggt gccgcaatcc tcgacgcgtt ggaggctatc
     1141 cagagagtga cggggtcgca gagcgcccat gttcaggcgt cgtgctcggg aggcatcctg
     1201 gcggccatga cggcggcaca tctcagtgcg acgggtgagg ctgagcgcat cgccagcctg
     1261 accttgatgg tgaccgtcct ggatcaggga cgggagggct tggccgctgc cgtcgacgag
     1321 acggccgcca aactcgcgat tgcgttctcg gcgcgcaaag gcttcctcga cggccgcgca
     1381 ctggccgagg tgttcgcctg gttgcgtccc accgatctgg tttggcggta ctgggtcaac
     1441 aactatctgc agggccggtc accggctccg tttgacgtgc tgttctggaa ctccgacacc
     1501 acccgcatgc ccgccgcctt gcaccgcgac atgttgttga tgggacagca caacgcgttg
     1561 gccgaacccg gcgcggtgcg catgctcggc accccggtgg acctcggcac ggtgacggcg
     1621 gatacctacg tcgttgcggg gatcgccgac cacatttcgc cctggcaggc ctgctatcgc
     1681 agcgcgagga tgttgggaag caaagatctg cggttcgtcc tttcatcgag cggacacatc
     1741 gccgcgctcg tcaatccccc tggcaacccg aaggcgtcct accgcgcggg aaacgtcgac
     1801 gaacgcgacc caacacgttg ggcggcgtcg gcggaaacgc acagcgattc ttggtggccc
     1861 gacttctccg cgtggttggc gcagcgcggc ggcgagcaga agccggcgcc cacggcgctt
     1921 ggcggtgacg gtttggcggc gctgggtccg gcgccgggca gctacgttca tgtccgttaa
     1981 ccgcctcgcg gtgttgccga tccgagtcct tggggcattg gagtcaagct gcgcctgcgt
     2041 cagctgcgca gcctcggcga gcgccgaagt cttcgccgcc gcgagatcgc acctgctccg
     2101 ggtggcggcc ggtccgccgc ccccgaccga cacggagggc attgccgatg tcatcgtgct
     2161 ctccccgggg cacaagcatg aacgatgaaa accttgtcgc ggtactcggt cagcagatcc
     2221 gcgtcgacat ccggtggggc accggggtcc cgttggtgct gtgcaacgga atcggggcaa
     2281 gccttgaagt tctcgatccg tttgtcgcgc aactggatcc ggacaccacc gtggtgcggt
     2341 tcgacgtgcc gggctccggc gcctcgccgg attcaccgtt tccgtaccgg ttcccatact
     2401 tggccgcagt gctgggaacg cttctgaaga aaattgggat catcgatcgg cgagccggta
     2461 tatcgcaggt tgacgtcttg ggactgtcgt ggggcggagg gctcgcccag cagttcgcct
     2521 ttcagaaccc gcgccggtgc cgcaggctca tactcgtatc cacaggtacc ggttcgctga
     2581 tgattcccgg caaaccgccg gtgctggcga agatgttgac agcgaggcga ttcctggatc
     2641 atcgctatgc cgcttcgatc gcgcctgaga tctatggcgg gaccgctcgc agcgacccga
     2701 tgatgatcaa gcgcgtcttc gaccgccagc tgatggctgg ctcgcgcgtc gggtatttgc
     2761 accagctgct tgccggtgca gggtggacca gcgttttcat gctgccgctg gtccggcagc
     2821 ccaccttgat cgtcgcgggt agtgacgatc ccatcatccc gctggttaac gccaggatca
     2881 tgaaccggct cttgccgaat tctgcgctac atattcacga cggtggccat gttgaactca
     2941 tcaccaacgc ggccgagcag gcacgggtga tcgaagagtt ccggcgcaag gcctaatccc
     3001 gaaccctgtc aatctaattc gacagcgagt tcaaggcgcc gaggttgccg ggcgatggca
     3061 ggtgccagca caggcgagcg cgaccgccag gtgcgaatgg cgtgtacaac gctgacaccg
     3121 actgggatga acagtgggga caacaccagc aggaacgtaa ggatgtgcgg gatgtacgac
     3181 atcgtgatcg gtcttcctct ccgccaagca gattgggaga agctagcccg ccaggtggac
     3241 gtgcggcttc gtcgtcgggc aaccaactct tcgcgcgagg ttgtgcggtg gcgacaatga
     3301 cggattccat tggcgctggg ccacaaccat tcctgacatt ttcgtgttgc aggcacgatg
     3361 gcgacgggcc gaaatacatc taatctgctc cacatgcaac gcttcggtga tccatcgttc
     3421 gacctcttca agctgtccga cgagcacaat gaactgcgcg ccgtgctccg cgatctgtgc
     3481 gagaaggaga ttgccccgta cgccgctgac gtcgacgaga gctcgcggtt tcccgaggaa
     3541 gcactcaagg tcctgaatga caccgggttc tcggcggtgc acattcccga ggagtacggc
     3601 ggccagggcg ccgacgcggt agcgggttgc attgttgtcg aggaggtcgc tcgggtgtgc
     3661 gcatcctcct cgctgatccc cggcgtcaac aaactcggca cgatcgggct gatcctcagt
     3721 ggctcagacg aattgaagaa gcaggtgctg ccgaccctgg catcgggcga cgcgatggcg
     3781 tcctacgcgc tatcggaacg cgaagccggc agcgatcccg cgtcgatgcg cacccgcgcc
     3841 cgcgccgacg gcgacgactg ggtgctcaac ggctccaagt gctggatcac caacggcggc
     3901 aagtcaacct ggtacacggt gatggcggtg accgatccgg agaagggcgc caacggcatc
     3961 tcggcgttcg tcgtccatga ggacgacgag ggcttcacgg tcggccccaa ggagcgcaag
     4021 ctcggcatca agggctcacc gaccaccgaa ctgtatttcg agaactgccg cattcccggc
     4081 gaccgcatca tcggcgagcc gggcaccggg ttcaagaccg cgctggccag cctcgatcac
     4141 acccgaccga cgatcggcgc gcaggccgtc ggcatcgcgc agggagcgtt gcacgcggcg
     4201 atcgcataca ccaaggaacg caagcagttt cgcaggccgg tgagcgactt tcagggcgtc
     4261 cagttcatgc ttgccgacat ggcgatgaag gtggaggctg cgcggttgat ggtgtacagc
     4321 gcggccgccc gcgccgagcg tggcgaggcg gcgctgagct ttatctcgtc ggccgcgaag
     4381 tgctttgcct ccgacgtcgc gatggaggtc accaccaacg ccgtccagct gttcggcggt
     4441 gcgggctaca ccgtcgactt cccgatcgaa cggatgatgc gcgacgcaaa gatcacgcag
     4501 atctacgagg gcaccaatca gattcagcgc gtcgtcatgg cgcgggcatt gctggcgtga
     4561 ctcctcgggc acgtgcggcg actgctcgtg gtgattggta gacctttgag cgtcgatcgc
     4621 gtcgtcagtg ggcggatcgg cgatatcgtg tcattacagc aacggaggcg atgccccccg
     4681 gctgaggata gtgaccgtcg atggccgacg ccgacccgca ggcaacccag cgggatgtgc
     4741 tcacaggcat ccccgcggaa ctgtccatct ctggattcga tgatgtcgtg gaaatcgggc
     4801 acggcggatt cggggtggtc tatcgctgcg ttcagccgtt gctggatcgc gacgtcgcca
     4861 tcaaggtgct gaccggcgac ttggaccccg acaatctcga ccgattcctt cgcgagcagc
     4921 gcgcgatggg ccggctatcc gggcatccgc acatcgtgac catattccag gtcggcacca
     4981 cagccggggg gcggccgtac atcgtcatgc cctgccaccg caagggctcg ctggaagcgc
     5041 tgatccgcca gcacggtccg atggattggt gtgagaccct gcgcatcggg gtgaagctgg
     5101 cgggtgcgct cgaagccgcc catcgcgcag gcatactgca ccgggacgtg aagccgggca
     5161 acgtcctgct taccgagtac ggcgaacccc agttgaccga cttcggtatc gccaggatcg
     5221 ccgggggttt ccagacgggg accggagtaa tcatcggttc accggcgttc accgcgcccg
     5281 aagtgctcgc gggtgcagcc ccgacgtccg catcggacgt gtactcactt ggcgcgacgc
     5341 tgttctgcat actgaccggt cacgcagcgt ttgagcgtcg cagcggcgag caggtggtcg
     5401 ctcagttcct ccggatcacg tcacaaccga taccggatct gcgcgagcag gg
//
LOCUS       DASRJA010000698        31743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3781525,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000698 DASRJA010000000
VERSION     DASRJA010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 31743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31743
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3781525"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2329
                     /locus_tag="VFC01_31425"
     CDS             <1..2329
                     /locus_tag="VFC01_31425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN84165.1"
                     /translation="PQTTSVPLAARPTHSYPSPPPQPPTSRPPVPSHPQQPVYPSGPQ
                     PYSSGPQPRYPSGPAAGYPSGPSTYPPSTSQPQPARPAAVAHPSISSPSLESVTQLGP
                     TAAPRTSEGNLATSMLKILRPGGPAKAPAGSTKIGRATDNDIVIPDVLASRHHATLIP
                     GPSGTEIRDERSINGTFVNGARVDSAMLKDGDTVTIGNVDLVFRGGTLVRRTETEAAT
                     RTGGLEVHGVTWTIEGNKTLLDNISVAARPGMLTAVIGPSGAGKSTFARLVAGYTHPT
                     TGTVAFEGHDVHAEYASLRSRIGMVPQDDVVHGQLTVRQALMYAAELRLPPDTNKADR
                     EKVVYEVLEELEMTKHLDTRVDKLSGGQRKRASVALELLTGPSLLILDEPTSGLDPAL
                     DRQVMTMLRQLADAGRVVLVVTHSLTYLDVCDQVLLLAPGGKTAFYGPPDEIGPSMGT
                     TNWADIFSTVAGDPDGANKRFIAQHGPPPPQPAAQKPADLGKPTKTSLRRQFSTIARR
                     QMRLIVSDRGYFIFLAVLPFIMGVLSLSVPGDVGFGVPVPAIKGGEAPNEPGQILVLL
                     NVGAIFMGTALTIRALIGERAIFLREQAVGLSTSAYILAKVFVFAGFALLQSGIVVAI
                     NIWGKKWGPGAVTSGAVIPPRTLELYVDVAACCVGAAMTGLALSALAKSNEQIMPLLV
                     IAIMSQLVFQGGMIPVTGRIVLDQLSWVTPARWGFASTASTIDLLRLVPGPLTPQDSH
                     WRHTPGAWWFNMAMLGAICVGYISFVRWKIRLKGG"
     gene            complement(2331..3515)
                     /locus_tag="VFC01_31430"
     CDS             complement(2331..3515)
                     /locus_tag="VFC01_31430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN84166.1"
                     /translation="MTSTAEVGSPVVARARGMRDLVRTEAAESERLRTLTPAIVDEMW
                     ASGLMSSFNPIPAGGVEPSFTEMIETWIEMAWQDGSFGWCGIANLPSTFAAGAYLPDD
                     GFAEVFTAHDNRITMGGQFFPNGQGQAVDGGYRLSGSWSFGSGTGHSQYIAAGFMPMD
                     NGEVRWISEGLPDMRVAIVPRDDITFTDGWFVQGLKGTGSYDYNAVDVFVPQYRTFPL
                     FARDPHRGSSPATRMGLMPVTAAGHASWALGVAKSMLDDVEELAATKFRMSDMASLAS
                     RPTFQKGLAHHVASWRAARLLVLEEFTRAEAAVAAGDDLTPALRADMRVAAVYATDVA
                     RACGEWAHLVAGTSSIREGSRLERAFRDLYTGTQHAFISEKVAIDAAQIWLGIIEDQP
                     GL"
     gene            complement(3542..3898)
                     /locus_tag="VFC01_31435"
     CDS             complement(3542..3898)
                     /locus_tag="VFC01_31435"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN84167.1"
                     /translation="MSTRRIIETWVERFNAGDAAGISALYAKDAVNHQIPLTPIVGRA
                     AIEEFHRETFAGGPLTCTPINLVVDGEWGALEWTDPDGFRGCGFFHVRDGLIAQQRGY
                     WDSAQLAEVHPNMHGG"
     gene            complement(3921..4835)
                     /locus_tag="VFC01_31440"
     CDS             complement(3921..4835)
                     /locus_tag="VFC01_31440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN84168.1"
                     /translation="MRAVIYDPDARANLRLADVDEPVVADSEALIEVRATALNFGEVH
                     FIDRMRKPGEVPGWDAAGVVLQAAANGSGPPVGTRVVGFDGAGGWAQRRAVSTENLAV
                     LPDSVDFGQAAALPVAGVTALQALRRLGPVVGRRVLVTGASGGVGRFAVQLAARAGAH
                     VIAAVGSAARGAGLADLGAAEVVVGLADVTEPVFGVLDNVGGQLLAEAFSLVADGGSA
                     QSIGMASNQPTTIDFEAQRQIPGHRRLEPFTVRTPFAPDLAYLVELLADGEIDPQIGL
                     RTSWTDVSEAAEALLDRRVAGKAVLDVD"
     gene            complement(4871..6145)
                     /locus_tag="VFC01_31445"
     CDS             complement(4871..6145)
                     /locus_tag="VFC01_31445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN84169.1"
                     /translation="MAATWDTIDRYVVISTDTHAGADLYDYKPYLPTRLHDEFDAWAK
                     TYESPFDDLIVATAKRNWDHELRMSEMDDDGVAAEVLLPNTIPPFFPTTPNITISLPT
                     TRDEFEKRWAGVQAHNRWQIDFCALAPARRRGLIQIFPNDIDLALDEIRWGVEKECFG
                     GVLLPPVSPGDPTVAPLFHTRYEPIWALCEDLDLTVVQHAGPGSPAMPMDQPASNAVL
                     VSEMAMWAQRTVSHLVLAGVFERHPTLRFVPTEQGTMWLQQQMMLLDAMVPSMKTEAH
                     NRTYGMFGGSSIDQLTLAPSDYAKRNCYLASELMPFESSMIDYMGADHIMWGSDYPHE
                     EGFAPHSKLAIRWALHDRSEDVCRKILAGNAGRLYRFDLEALAPVAAEIGPTVAEVHT
                     PLGDTGYVAPAAFGYRPFEGGLALKRLAPARS"
     gene            complement(6211..6639)
                     /locus_tag="VFC01_31450"
     CDS             complement(6211..6639)
                     /locus_tag="VFC01_31450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN84170.1"
                     /translation="MPAPRWVARANKVGLNRLTKFIAPWAPGWAVVIHRGRKSGRSLR
                     TPLWAFRRRGGYVIALTYGSSADWVRNVLSAGGCELVSRRRHYQLGAPRLYRDENASD
                     MPAFIRFMLRRVIKAPEFLSLDVVGELPTEISSHSGVVSS"
     gene            6727..7308
                     /locus_tag="VFC01_31455"
     CDS             6727..7308
                     /locus_tag="VFC01_31455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN84171.1"
                     /translation="MARIGRPRGKTDTRNVILAAARHLFADAGYDKTSVRDVATAAGV
                     DPAMIRHYFGSKAELFRATMGWPFEPAEIAARVTGGGRGEIGARLTRVFFEAWEQPNS
                     RAPLLAILRGAATHDESANLVRQFIQGQVYSHIAAALAEPDAELRIDLAMAQLLGIAY
                     LRHILRVEPVASTPVEDLIQRVAPAITRHLTPP"
     gene            complement(7378..7941)
                     /locus_tag="VFC01_31460"
     CDS             complement(7378..7941)
                     /locus_tag="VFC01_31460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZN84172.1"
                     /translation="MGDNETVWVEREFDGHDFRDDDLSQLRTERVVFTECDFSGVDMS
                     ESEHVGSAFRNCTFRRTSLLHSTFRHCSFLGSVFTECRIRPIKLVEVDLTLTVLGASD
                     LRAVDLSDCRLREASLVEADLRKAVLRRADLSGARLQNAKLDEADLRGARVDPTLWTT
                     ASLRGAKVDIEQAMAFAAAHGLDIHGE"
     gene            complement(7934..8515)
                     /locus_tag="VFC01_31465"
     CDS             complement(7934..8515)
                     /locus_tag="VFC01_31465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HZN84173.1"
                     /translation="MAYERSERRNTASTKIVISGGFGAGKTTFVGAVSEIMPLRTEAL
                     VTQQSEGVDGLEATPQKNTTTVAMDFGRITLADDLVLYLFGTPGQRRFWFMWDDLVLG
                     AIGAIILVDVRRLQDSFAAVDFFEARNLPFLVAVNEFDGAPRHPAHAVRKALALREDI
                     PVITVDARDRHSTKAALIAVTEYALNTLTPVRG"
     gene            complement(8496..8870)
                     /locus_tag="VFC01_31470"
     CDS             complement(8496..8870)
                     /locus_tag="VFC01_31470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HZN84174.1"
                     /translation="MDHWERDDTDEPQLVRPYTLTAGRTDSRIYLPLEAPIETVADPP
                     KPPRWPGNDVRGQILTRCAESPSVAEIAAVLSLPLGVARVLIGDLVTQGYLRVHTTLG
                     DSTTVDERRELIGRTLRGLRAL"
     gene            complement(8878..9294)
                     /locus_tag="VFC01_31475"
     CDS             complement(8878..9294)
                     /locus_tag="VFC01_31475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HZN84175.1"
                     /translation="MTYPPRSTQRDSLDWLVSKFAREVSGVSHAVLVSADGLLMAASE
                     HMPTERADQLAAVASGLASLSTGASQLFDGGYVLQSVVEMENGYLLLMRVGDGSNLAT
                     LATRNCDIGQIGYEMAILVERVGAMAQAARRAPQHS"
     gene            complement(9291..12164)
                     /locus_tag="VFC01_31480"
     CDS             complement(9291..12164)
                     /locus_tag="VFC01_31480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN84176.1"
                     /translation="MTAFSQLKQADGTLVDFQPKKQPPAKRPSRWSIANWPVRWKVFA
                     IVLVPLVLAGTFGGLRIYSSATEATDLRRAADRAEMVPAIVNYMASLDAAMLASSTGG
                     DTQAALSKFDASRQELQRRLTDTDIAPDVSKGVSTMIGAGQGLIDKVTANSVGLRDRV
                     QAYTPILLTAEDAINGSVRVDDERIRAETLGLSRAVGARGQMMIQQLLVSLGGELPDP
                     ELRMSMNTVAGTEPSTLFGMSQVLGVGSPEAQKLQEEFVKRMTIMSDPAAVLVNNPDL
                     NASIQVTDQIATKIIDDTSSAVTGAVERETAAQRTGAIRDSAIVLGAMLLALLLVILV
                     ARSLVRPLRQLRDSALQVAHDDLARELDRVRAGGEPGHVQPIPVYTSEEVGQVAHAVD
                     ELHEQAVLLAGEQSRLQLQVSDMFETLSRRSRSLVDQQLSLIDRLERNEEDPERLESL
                     FRLDHLAARMRRNGANLLVLAGAKVPREQADPVPVSAIINAAASEVEDYTRVVTATVP
                     DSEVVGALAGDLVHLLAELLDNALRYSPPISQVRVSAVHTGNGGLVIEVSDIGIGMTE
                     PDLRVANTRLQSGGEVNPYTARHMGLFVVGRLAKQHGLVVRLQSTIAGEPNSGTTAGV
                     FVPSELLAVAGAPDQFGGPEYAVPADAHAGIHTALALDEEHEYTFDEPGHEYTNGHPE
                     VPIELLPQRNPGASGIADVPASLAQPEKGDDEWPAEELWPEDSMPVHTAEPNPWEQPA
                     AQEPEADRAPIDTSAFFASRAQAVSNGVPTPPRGEQPRPEAPSQHEPPAVAEQPQPEP
                     SRHAVRESSPTAGADDAIYQKMLSEWLVDPTEIAKSDDLNWESVWDHGWSAAAAADEA
                     PVTEHTEEGLPVREPGARLVPGAVDSAVPDAPSNGGHHFNGGAHRSDDDADEVASAAG
                     FGTGGGPIPPRDPDAVRASISNHFGGVHAGRSHAQETRGTDNE"
     gene            12478..12636
                     /locus_tag="VFC01_31485"
     CDS             12478..12636
                     /locus_tag="VFC01_31485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84177.1"
                     /translation="MTEPEKFEVDPSGDPNPGVADHALPDDAKGPHPLADPANDPTPG
                     VPGHAKDD"
     gene            complement(12755..12946)
                     /locus_tag="VFC01_31490"
     CDS             complement(12755..12946)
                     /locus_tag="VFC01_31490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84178.1"
                     /translation="MSAVVVVALVIVAVIIAAGIVVLVIVLREAWPSRGEWAEEDRAA
                     GEDPRRGLAEDFWAGPPGM"
     gene            13040..13504
                     /locus_tag="VFC01_31495"
     CDS             13040..13504
                     /locus_tag="VFC01_31495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HZN84179.1"
                     /translation="MFRLMFYSPRIAPNTGNAIRMVAATGCELHLVEPLGFDLSEPKL
                     RRAGLDYHDLASVSVHRDLPAAWEAVKPKRVYAFTAHATTSFADVSYQPGDVLMFGPE
                     PTGLDAATLADPHISAQVRIPMLVGRRSLNLSNAAAVAVYEAWRQNGFTGAV"
     gene            complement(13505..14146)
                     /locus_tag="VFC01_31500"
     CDS             complement(13505..14146)
                     /locus_tag="VFC01_31500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZN84180.1"
                     /translation="MTLNLSVDEVLTTTRSVRKRLDFEKPVPREVLTECLDLALQAPT
                     GSNAQGWQWVFVDDPEKKKALADIYSTNATPYLDLPKPEYGDVRDEQRPRVYGSAKYL
                     NEHLHEAPVMLIPCLEGRPDGAPAGMSASFWGSLLPAVWSFMLALRSRGLGSAWTTLH
                     LLGDGEKQAAELLGIPFDQYSQAGLFPIAYTKGTDFRRADRLPAEQVTHWDTW"
     gene            14170..14586
                     /locus_tag="VFC01_31505"
     CDS             14170..14586
                     /locus_tag="VFC01_31505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HZN84181.1"
                     /translation="MTVELTQEVSDRLSSDPYGWLTTVAKSGQPVPRLVWFYFDGTDV
                     IVYSEPNAAKVRHIKAHPRVSLNLDSDGNGAGIIIVGGNAMVDADGADPLQDEQYRAK
                     YGELAASFGFSEEFLAAYNTRLKISIDKVWTTPTEG"
     gene            complement(14592..17885)
                     /locus_tag="VFC01_31510"
     CDS             complement(14592..17885)
                     /locus_tag="VFC01_31510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009956978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HZN84182.1"
                     /translation="MSWFTGPPSWTEMERVLNGKPRRAGWPVDQQVGDGGDSPAWSRK
                     RGEYQPLDVERPVSSVPYAELHAHSAYSFLDGASTPEELVEEAARLDLRAIALTDHDG
                     LYGVVRFAEAAKELEMQTVFGAELSLGGGDRTEDPDPPGPHLLVLARGPEGYRRLSRQ
                     LATAHLAGGEKGKLRYDYDALTEAAGGHWHILTGCRKGHVRQALSTGGPEAAGRALAD
                     LVDRFGAGRVSIELTHHGHPLDDERNVALAELAPRFGVNVVATTAAHFAAPSRGRLAM
                     AMGAIRARNSIDEAAGYLAPLGGSHLRSGEEMARLFRGAPEAVTAAAELGEQCAFGLA
                     LIAPQLPPFDVPEGHTEDSWLRHLVMEGARGRYGPPERAPRAYAQIEHELKIIEQLTF
                     PGYFLVVHDITRFCRDNNILAQGRGSAANSAVCYALGVTNVDPIANELLFERFLSPAR
                     DGPPDIDIDIESDLRENAIQYVYDRYGRDYAAQVANVITYRGRSAVRDMARALGFSQG
                     QQDAWSKQIGQWGNLSESTHVEDVPESVIDLALQISNLPRHMGIHSGGMVICDRPIAD
                     VCPVEWARMENRSVLQWDKDDCAAIGLVKFDLLGLGMLSALHYCIDLVREHKGIDVDL
                     AKLDLSEPAVYEMLQKADSVGVFQVESRAQMATLPRLKPRVFYDLVVEVALIRPGPIQ
                     GGSVHPYIKRRNGVEPVTYDHRSMAAALRKTLGVPLFQEQLMQLAVDCAGFSAAEADQ
                     LRRAMGSKRSTERMRRLRGRFYDGMRERHGITGDVADRIYEKLEAFANFGFPESHSLS
                     FAALVFYSSWFKLHHPAAFCAALLRAQPMGFYSPQSLVADARRHGVTVHGPDVNASLP
                     HATLENEGLEVRLGLGAVRHIGDELAEQIVEDRKANGPFDSLLDLTGRIQLSVPQTEA
                     LATAGALGCFGITRREGLWAAGAAATQRPDRLPGVGSSSHIPSLPGMTELELAAADVW
                     ATGISPDTYPTQFLREDLEAIGVVPADQLLAVPDGTRVLVAGAVTHRQRPATAQGVTF
                     INLEDETGMVNVLCAPGVWARHRRLAQTASALLIRGQVQNATGAVTVVADRIGKLSMS
                     VGSKSRDFR"
     gene            17926..18804
                     /locus_tag="VFC01_31515"
     CDS             17926..18804
                     /locus_tag="VFC01_31515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN84183.1"
                     /translation="MISAFASGQHGMGTKRSRSGHLPINGLNLYHEVYGELGKSKVPP
                     LLLIPGAFMATDSMKSWVSAFAGKRAVIVFDQQGHGRTPDTSRKMSYEQFADDAAELL
                     RALKVERADVMGYSQGGGVALQLALRHPTLVDKLVSLSATYRQDGWYPSVLKGIEGLT
                     AEAFAGTPVEKAFKEHTPDAKAYGAYLKKMKVLNIQDQNISDAQMRSMSAKTMVIVGD
                     ADGVKPEHAVAMFKLRGGGDEEAAATGTLQKVPAARLVILPAMSHIGISAETAVLVPM
                     VSAFLDDVPPTTPDLF"
     gene            18871..19569
                     /locus_tag="VFC01_31520"
     CDS             18871..19569
                     /locus_tag="VFC01_31520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HZN84184.1"
                     /translation="MTKQTATALPPVVDQDTWRAALDDLRRREKAATRELDAIAAQRR
                     RLPMVEMPDYTLIGKDGTVRLADVFDGHSQLIVYNHMWEDGAEWQCGGCTGLTAGWTR
                     LEFLANYDARMVIVTNGPIEEALAYKQKVGNKMEWYSSSESSFGADVDAPPGGGFAVN
                     VFLRDGDKVYRTWHTNGRGTEQLTYTFGLIDLLPWGRQEEWQDSPDGWPSRPTYSGWL
                     DSPDIARVYGRTGG"
     gene            complement(19566..20450)
                     /locus_tag="VFC01_31525"
     CDS             complement(19566..20450)
                     /locus_tag="VFC01_31525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZN84185.1"
                     /translation="MKLVVGYLATPGGADAMALAARLARTLGADVEACIILPPDRVLP
                     AASTRDYETKLGEQAEKWIAEAIASVPDDLTVHGHVSFDDSFADGLIREAARLEADLI
                     VVGGSGGGLAGPYSLGSVVNELLHSAPLPVVVAARGTRDTKIDRVREVTCALGQRQGA
                     DLLLEKAVRFSKAAGTPLRLVSLVALDPIFGQLRGDEEAVRERALTHAQQVLDTAKSS
                     LPEDFPVAATAVEGATVEEAVRKLEWHDGDLIMVGSSRLSAPKRLFLGSTAAKMLRVL
                     DTPMMVVPRDQFGAQDLP"
     gene            complement(20543..21412)
                     /locus_tag="VFC01_31530"
     CDS             complement(20543..21412)
                     /locus_tag="VFC01_31530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZN84186.1"
                     /translation="MPIDPNAIGAKTDAQLFKWTDRDTLLYALGVGAGIDDLAFTTEN
                     SHDTPQQVLPTYAVIACLPFAAAAKIGSFNFAMLLHGSQEIRLFKPLPPAGKLSVVAE
                     VADIQDKGEGKNAIVMLKATGTDPDTSEVIAETLTTAVIRGEGGFGGQPGQRPVAPEI
                     PDREPDARIALPTREDQALIYRLSGDRNPLHSDPWFAQNLAGFPKPILHGLCTYGVAG
                     RALVSELGDGDATKVSQVGARFTSPVFPGETLTTSIWRTESGHAVFRTEASNPDGSDA
                     RLVLEDGTAEYAA"
     gene            21503..23509
                     /locus_tag="VFC01_31535"
     CDS             21503..23509
                     /locus_tag="VFC01_31535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN84187.1"
                     /translation="MRYVVTGGTGFIGRRVVSQILGRHADAEVWVLVRRGSLTRFERL
                     AGEWGPRAKALVGDLTASSLGLTDDAVAELGTVDHVVHCAAIYDITAKEADQQAANVE
                     GTRAVIDLARRLDATLHHVSSIAVAGTYHGEFTEDDFDVGQDLPTPYHQTKFEAEMLV
                     RSAAGLRYRVYRPAVVVGDSRTGEMDKVDGPYYFFGLLAKLALLPRFTPMVLPDTGRT
                     NIVPVDFVVDALVELMHADGRDGQTFHLTAPKTIGLRGIYRGVAEAAGLPPLRGSLPG
                     ATAKPFLRVTGRAKVLRNMAATQLGIPGEILDVVDLAPTFTSVNTTEALRGTGITVPE
                     FASYAPRLWRYWAEHLDPDRARRDDPAGPLVGKHVIITGASSGIGRASAIAVAERGAT
                     VFALARNAEALDDLVAEIRAVGGQAHAFTCDVTDATSVEHTVKDILGRFGHIDYLVNN
                     AGRSIRRSVIASTDRLHDYERVMAVNYFGAVRMVLALLPHWRERRFGHVVNVSSAGVQ
                     ARNPKYSSYLPTKAALDAFADVVSTETLSDHITFTNIHMPLVKTPMIAPSRRLNPVPP
                     ISPEHAAAMVVRGLVEKPARIDTPLGTLADLGNYLTPKLSRRVLHQLYLGYPDSAAAR
                     GLVPEPSTAPAPSRRPRSPAQRVTPLRVPGPIKRAVRLVPGVHW"
     gene            complement(23515..23904)
                     /locus_tag="VFC01_31540"
     CDS             complement(23515..23904)
                     /locus_tag="VFC01_31540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN84188.1"
                     /translation="MTGRVVHFEVPFSDGDRARNFYKQIFGWQLNEMPEMRYTIVSTG
                     PSAESGMPAEPGYIGGGMFEREPAFPQGPVITIDVDSIDDALAKIVSLGGEPVGEKTP
                     VGEMGFAAYFKDTEGNIMGLWETASSA"
     gene            23995..25059
                     /locus_tag="VFC01_31545"
     CDS             23995..25059
                     /locus_tag="VFC01_31545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HZN84189.1"
                     /translation="MTVGGEGPGGPLPVFVDVDTGVDDAMALVYLFASEDAEVVGIAS
                     TSGNVPVQQVCRNNLGLLDLCRITGVPVSKGAEQPISTPLRTAEDTHGPEGLGYAELP
                     PTDGQLTAHDAAEAWVRAVHAYPGELVAIATGPLTNLALALRLEPALPKLLRRLVIMG
                     GAFDYRGNTTPVAEWNISVDPEAAAEVFAVWGAAWGLEPPKHIPIVLGLNLTENVAMT
                     PTILSRLATAAESSSVAMSVLDDRGTRSVASNPLIRVLEDAMRFYFEFHFDQGEGYLA
                     HLHDPLSAAVALDPDLVQYRPAAIDIELTGTLTRGMTVADWNSHWGREPNALIGIDVD
                     PGVFFDRFIARVGRFAQRLG"
     gene            complement(25127..25549)
                     /locus_tag="VFC01_31550"
     CDS             complement(25127..25549)
                     /locus_tag="VFC01_31550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016893308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HZN84190.1"
                     /translation="MKTLYTAEALTTGEGRDGHGRTSDGKLDLDLAIPKEMGGSGNGT
                     NPEQLFAVGYAACFHSALRLVGRQENADVSDSAVGARVSLGQIDNGGFGLAVELEVTL
                     PNLDHDAAQQLTEKAHQVCPYSNATRGNIDVKLVVTDD"
     gene            complement(25546..25992)
                     /locus_tag="VFC01_31555"
     CDS             complement(25546..25992)
                     /locus_tag="VFC01_31555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN84191.1"
                     /translation="MAMLSLDRQLCFALYSASRAMTAAYRPILTELNLTYPQYLVLLV
                     LWEEDRVTVGRLGERLQLDSGTLSPLLKRLEANGFVRRERSLDDERLVDVTLTPAGRR
                     LERKAQCIPEQLFSSTGMTEQEAADLRDAVRHLTDALNASQRKESA"
     gene            complement(26036..26596)
                     /locus_tag="VFC01_31560"
     CDS             complement(26036..26596)
                     /locus_tag="VFC01_31560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN84192.1"
                     /translation="MTDIIDPTKTFRLLEQRIAETTNPRHLLMLNRVLQHAMGEAKPD
                     LDLVMSTLCPDPRYIAWGAPEDFSPVGRQAVRAFYDETIVKGGQFCLELDIDRIVVDD
                     DTVVTEGNFRSISYGADAVKRGFPADDPAGFYLLQLRMLVVWPFDAEGFIKGEESYSA
                     ITTPDFFTKLDASQVPQAFRDYIATR"
     gene            complement(26653..28191)
                     /locus_tag="VFC01_31565"
     CDS             complement(26653..28191)
                     /locus_tag="VFC01_31565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HZN84193.1"
                     /translation="MDWPAVAALAAAGLAPTTPVAVTLANRVIACSASARAAGVRRGL
                     RRRESQARCPELYVVTADPARDARHFEVVTAAVDDLVPRAEVLRPGLLVLSVRGAARY
                     FGSEQAAAERLVDAVAAAGAECQVGIADQLSTAVFAARAGRIVEAGKDALFLSALSIR
                     QLATEPSLAAPGREDLADLLWRMGIRNIGQFAALSRSDVASRFGADAIAAHRFARGEP
                     TRGPSGREPPAELDAVMNCDPPIDRVDAAAFAGRSLASTLHRSLEAAGVGCTRLAIHA
                     VTTSGQELERVWRCAEPLTEDATADRVRWQLDGWLNKRHPDDRPTAPVTLIRLRPVEV
                     VSAEALQLPLWGGIGEEDRLRARRALVRVQGLLGPEAVQVPVLSGGRGPAERITFTPL
                     GDEPVPRADPSQPWPGQLPEPSPTVLLDDPVELLDADGNPVRVTGRGLFSADPWLLAG
                     PGREDRLAWWTGPWPVDERWWDSEQAKAGRTARAQVLLDGNPAQALLLCYRQRRWYVE
                     GIYE"
     gene            complement(28234..28905)
                     /locus_tag="VFC01_31570"
     CDS             complement(28234..28905)
                     /locus_tag="VFC01_31570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84194.1"
                     /translation="MSLEQRLLDRADQVEHLRRKIAAVSSKVENGRRGPAPSIDPLPD
                     SESLLPLPEALAELLPTSLPRGTVAVLSGARSLALSMVAAVTAEGGHAAIVGQPDVGL
                     LAAVEMGADLSRIAVIPEPGADPVEVAAVLMDGMDLVVLGLGGRTVPAGRARAVVARA
                     RQKGCTLLVTGGDWQGASNRLEARVCGYEITGPGRPGIGRISKVRLAMRARGRSFGTV
                     RAVGG"
     gene            complement(29036..30622)
                     /gene="guaA"
                     /locus_tag="VFC01_31575"
     CDS             complement(29036..30622)
                     /gene="guaA"
                     /locus_tag="VFC01_31575"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZN84195.1"
                     /translation="MESASPRPVLVVDFGAQYAQLIARRVREARVYSEVIPHTASVED
                     IKDKDPQAIVLSGGPSSVYEEGAPQLNPAVFDLGVPVFGICYGFQAMAQALGGTVAKT
                     GTSEYGRTDLKVSGGQLHSDLPGTQPVWMSHGDAVTAAPDGFDVVATSAGAPVAAFED
                     RARRLAGVQYHPEVMHSPHGQQVLSRFLHEFAGIGATWTPANIADSLVEQVRNQIGNG
                     RAICGLSGGVDSAVAAALVQRAIGDRLTCVFVDHGLLRAGERAQVQRDFVAATGANLV
                     TVDVADRFLEALAGVTNPEGKRKIIGRQFIRAFEGAVRDIVAGDWGHLPLAGDGSEVE
                     FLVQGTLYPDVVESGGGTGTANIKSHHNVGGLPDDLKFKLVEPLRLLFKDEVRAVGRE
                     LGLPEEIVARQPFPGPGLGIRIVGEVTAARLDTLRRADAIAREELTAAGLDNQIWQCP
                     VVLLADIRSVGVQGDGRTYGHPIVLRPVSSEDAMTADWTRVPYEVLERISTRITNEVA
                     EVNRVVLDVTSKPPGTIEWE"
     gene            complement(30675..31292)
                     /locus_tag="VFC01_31580"
     CDS             complement(30675..31292)
                     /locus_tag="VFC01_31580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN84196.1"
                     /translation="MAPLVAPRRPANGSQLRADRTRALVIGETIRCILDEGFAAASAK
                     HITERAGVTWGVVQYHFGDRDGLLMAVVDKGFAELLEALRELPAAAENVRDRTEFIVD
                     AAWEAFSSPTSMAALEILIATRAMRDPGASEHLAALGTAMAKLGKKIGEGLDARHAAA
                     IGNLIWATLRGLVVNALVIPEPLDTRRERRALVEMITSYVDGATV"
     gene            complement(31298..>31743)
                     /locus_tag="VFC01_31585"
     CDS             complement(31298..>31743)
                     /locus_tag="VFC01_31585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HZN84197.1"
                     /translation="SLADGLGVELDDVRERHETWVTPQSIECAMMTVPAGGVAAVRFA
                     VEGVRAGEPVITMEHVNRLTDVAAPDWPYPPDGRPGVHRVTVAGDPGVEINAHVGLGG
                     VDHNQGGVIATAARAVNMIEPVCTAPSGLLAARDLPAAHIRGVMW"
BASE COUNT         5337 a        10740 c        10330 g         5336 t
ORIGIN      
        1 gccgcagacc acatcggttc cgctcgcggc gcggcccacc cacagctacc cgagcccacc
       61 accgcagccg ccgacgtcac ggccgccggt tccgtctcac ccgcagcagc cggtgtaccc
      121 gtccggaccg cagccgtact cgtcggggcc gcaaccgcgc tacccatcgg ggcctgcggc
      181 gggatacccg agcggcccgt cgacgtatcc gccgagcaca tcacagccgc agccggcccg
      241 gccggcagcc gttgcgcacc cgtcgatctc ctcccccagc ctggagtcgg tcacccagct
      301 gggcccgacg gccgcgccgc gcacgtccga gggcaacctc gcgacgagca tgctgaaaat
      361 cttgcgaccc ggcggccctg cgaaggcccc agcggggtcg accaagatcg gccgagcgac
      421 cgacaacgac atcgtcatcc ccgatgtgct ggcgtcgcgc catcacgcga cgttgatccc
      481 cggtccgagc ggcaccgaga tccgcgacga gcgcagcatc aacggcacat tcgtcaacgg
      541 cgcgcgcgtg gactcggcga tgctgaagga cggcgacacc gtcacgatcg gcaatgtcga
      601 cctggtgttc cgcggcggaa cgctggtgcg ccgcaccgaa accgaggccg cgacacgcac
      661 cggcgggctc gaggtgcatg gcgtcacgtg gaccatcgag ggcaacaaga cgctgctgga
      721 caacatctcg gtcgccgcgc gccccggcat gctgacggcg gtcatcgggc cgtcgggtgc
      781 gggcaagtcg acgttcgcac ggttggtggc cggctacacc cacccgacga cgggcacggt
      841 ggcattcgaa ggacacgacg tccacgccga gtacgcctcg ctgcggtccc ggatcgggat
      901 ggtgccgcag gacgacgtcg tgcacggcca gctgaccgtc cggcaggcgc tgatgtacgc
      961 ggcggaattg cggctgccac ccgatacgaa caaggcagac cgcgaaaagg tcgtctacga
     1021 ggtactcgag gaactcgaga tgaccaagca tctcgacaca cgcgtcgaca aactctcggg
     1081 cgggcagcgc aagcgtgcat cggtggcgct ggagctactg accggaccgt cgctgctgat
     1141 cctcgatgag ccgacgtccg gcctggaccc ggcgctggac cggcaggtga tgacgatgct
     1201 gcgccaactc gccgacgccg gccgcgtggt gctggtggtc acgcactcgc tgacctacct
     1261 cgacgtatgc gaccaggtgc tgctgctcgc ccccggcggc aagaccgcgt tctacggtcc
     1321 gcccgacgag atcggtccgt cgatgggcac caccaactgg gccgacatct tcagcacggt
     1381 cgccggcgac ccagacgggg ccaacaagcg gttcatcgcc cagcacggcc cgccgccacc
     1441 gcagccggcc gcgcagaaac cggcagatct cggcaagccc acgaaaacca gcctgcggcg
     1501 ccagttctcg acgatcgctc gtcgccagat gcggctgatc gtgtccgatc gcggttactt
     1561 catcttcctg gcggtgctgc cgttcatcat gggtgtgctg tccctgtcag tgccgggaga
     1621 tgtcggcttc ggtgtccccg ttcccgcgat aaagggtggc gaggcgccca acgagccggg
     1681 gcagatcctc gtcctgctca acgtgggcgc catcttcatg ggtaccgcgt tgacgatccg
     1741 cgcactcatc ggcgagcggg cgatcttcct gcgtgagcaa gcggttgggc tgtccacatc
     1801 cgcgtacatc ttggccaagg tcttcgtctt cgccgggttc gcgttgctgc agtcgggcat
     1861 cgtcgtcgcc atcaacatat gggggaagaa gtggggtccg ggggccgtga ccagcggcgc
     1921 ggtgataccc cctcggaccc tggagctgta cgtcgatgtc gcggcctgct gtgtgggagc
     1981 ggcgatgacc ggcttggcac tctcggccct ggccaagtcg aacgagcaga tcatgccgct
     2041 gctggtgatc gcgatcatgt cgcagctggt gttccagggc ggcatgattc cggtgaccgg
     2101 tcgaatcgtc ctcgaccagt tgtcgtgggt cacgccggcg cggtggggat tcgccagtac
     2161 cgcgtcgacg atcgatctgc tcagactcgt ccccggcccg ctgacgccac aggactcgca
     2221 ttggaggcac accccgggcg catggtggtt caacatggcc atgctcgggg cgatctgcgt
     2281 cggctacatc agcttcgtgc ggtggaagat tcgccttaaa ggcggctgag tcacagacct
     2341 ggctgatcct cgatgatgcc gagccagatc tgggccgcgt cgatggccac cttctcactg
     2401 atgaacgcgt gctgcgtgcc ggtgtagagg tcgcggaagg cgcgctcgag ccggctgccc
     2461 tcgcggatcg agctggtgcc cgcgacaagg tgtgcccact ccccacaagc ccgcgccacg
     2521 tcggtcgcgt agacggcggc cacccgcata tccgcgcgca acgccggggt gagatcgtca
     2581 cccgcggcaa cggcggcctc ggcccgggtg aactcttcga gcacgagaag gcgagcggcc
     2641 cgccacgatg cgacgtggtg cgccaagcct ttctggaatg tcggacggct ggccagcgac
     2701 gccatgtcgc tcatccggaa cttcgtggcg gccagctcct cgacgtcgtc gagcatgctc
     2761 ttggccactc cgagtgccca cgatgcgtgg ccggcggcgg tgacgggcat cagacccatg
     2821 cgggtcgccg gcgacgagcc gcggtgcggg tcgcgggcga acagcgggaa cgtccggtat
     2881 tgcggcacga acacatcgac cgcgttgtag tcgtaggatc cagttccctt gagcccctgc
     2941 acaaaccagc cgtcggtgaa ggtgatgtcg tcgcgcggca cgatcgccac ccgcatgtcg
     3001 ggtaggccct cgctgatcca gcgcacctcg ccgttgtcca tcggcatgaa tccggccgcg
     3061 atgtactgcg aatggccggt gcctgagccg aagctccacg acccgctcag ccggtagccg
     3121 ccgtcgacgg cttgtccttg gccgttggga aagaactggc cgcccatggt gatccggttg
     3181 tcatgagcgg tgaacacttc ggcgaagccg tcatcgggca gataggcccc cgcggcgaat
     3241 gtcgacggca ggttggcgat gccgcaccac ccgaacgagc catcctgcca agccatttcg
     3301 atccacgttt cgatcatctc agtgaacgag ggctccaccc cgcccgccgg tatcgggttg
     3361 aacgacgaca tcaacccact tgcccacatt tcgtcgacga tggccggggt cagtgtgcgc
     3421 agtcgttcgg actcggcggc ctctgttcgt acgaggtcgc gcatgccacg cgcccgcgcg
     3481 acgaccggac ttcccacttc agctgtcgac gtcatggtcg atgaccgtac gctgtcgcgt
     3541 ctcatccgcc atgcatattc gggtgaacct cggcgagctg cgcgctgtcc cagtagccgc
     3601 gctgttgcgc gataagcccg tcgcgcacat ggaagaagcc gcagccgcgg aagccgtcgg
     3661 gatcagtcca ctcgagcgcg ccccactcac cgtcgacgac gaggttgatc ggcgtgcatg
     3721 tcaaaggccc gcccgcgaac gtctcccgat ggaattcctc gatggccgcg cggcccacga
     3781 taggtgtgag cgggatctga tggttgacgg cgtccttggc gtacagcgct gagatcccgg
     3841 cggcatcacc ggcgttgaac cgctccaccc aagtttcgat gatgcgacgc gtgctcatcg
     3901 cacgaagcta cgtgcggcca tcagtccacg tccaataccg ccttgccggc gacccggcgg
     3961 tcaagcaacg cttccgccgc ttccgacaca tcggtccacg acgtccgcag gccaatctgc
     4021 ggatcgatct cgccatccgc cagcagctcc acgagatacg cgaggtcggg cgcgaaaggc
     4081 gtgcgaacgg tgaacggctc caatcgcctg tgccccggga tctgccgctg ggcctcgaag
     4141 tcaatcgtgg tgggttggtt cgatgccatc ccgatggact gcgccgagcc gccgtccgcg
     4201 accaggctga acgcttcggc cagcagctgc ccaccgacgt tgtcgagcac accgaacacc
     4261 ggctcggtga cgtccgccag accgaccacc acctcagccg cgccgaggtc cgccaggccg
     4321 gccccgcgcg ccgcggatcc gactgcggcg atcacgtgtg cgccggcccg tgcggccaac
     4381 tgcaccgcga agcggccgac cccgcctgag gcgccggtga cgagcacccg tcgcccgacc
     4441 accgggccga gcctgcgcag cgcctgcaat gcggtcactc cggccaccgg cagtgcggca
     4501 gcctgcccga agtccaccga atccggaagc accgcaaggt tttccgtcga cacggcgcgt
     4561 cgctgggccc agccaccggc tccatcaaaa ccaacgactc gggtgccgac gggcggaccc
     4621 gaaccgttgg ccgcagcctg caggaccacg cccgcggcat cccatccggg tacctcgccg
     4681 ggcttgcgca tccgatcgat gaagtgcacc tcgccgaagt tcagcgcggt cgcgcgcacc
     4741 tcaatgagcg cctcggaatc cgcgacaact ggctcgtcga catcggccaa tcgcagattc
     4801 gcgcgggcat ccgggtcgta gatgactgct cgcatgccac cgtcaaaccc gaccgagcga
     4861 cggttattcc ctaactgcgt gccggggcaa gccgcttgag ggcaaggccg ccttcgaacg
     4921 gccggtagcc gaacgcggcc ggcgcgacat aacccgtgtc tcccaacggc gtgtgcactt
     4981 cggcgacggt cggaccgatc tcggcggcga ccggtgcaag ggcttccaag tcgaatcggt
     5041 agagccgccc cgcgttgccg gccaggatct tgcggcagac atcttcggac cggtcatgca
     5101 gcgcccaccg gatagccagc ttcgagtgcg gcgcaaagcc ttcctcgtgc gggtaatcgc
     5161 tgccccacat gatgtgatcg gcgcccatgt agtcgatcat cgaggactcg aacggcatga
     5221 gctcggacgc cagatagcag ttccgcttcg cgtagtcgct cggcgccagg gtcagctggt
     5281 cgatcgacga accgccgaac atcccatagg tgcggttgtg cgcctcggtt ttcatgctgg
     5341 gcaccatggc gtcgaggagc atcatctgct gctgcagcca catggtgccc tgctccgtcg
     5401 gcacgaaccg cagcgtcggg tggcgctcga acaccccggc aaggacgagg tgcgagaccg
     5461 tgcgctgcgc ccacatcgcc atctcggaga ccaacactgc gttggaggcg ggctggtcca
     5521 ttggcatcgc cggactaccg ggaccggcgt gctgaacaac agtcaggtcg aggtcctcgc
     5581 agagggccca gatcggttca tagcgagtgt ggaacagcgg tgcgacggtc ggatcacccg
     5641 gtgagaccgg cggcagcagc accccgccga aacactcctt ctcgacgccc caccggatct
     5701 cgtccagtgc cagatcgatg tcgttgggga agatttgaat caaccctcgg cgccgcgccg
     5761 gcgccagtgc gcagaagtcg atctgccagc ggttgtgggc ttgcacaccg gcccaacgct
     5821 tttcgaactc atcacgcgta gtcggcaggc tgatggtgat gttgggcgtg gtcgggaaga
     5881 acggcgggat ggtgttgggc agcagcacct cggcggctac cccgtcgtca tccatctcgg
     5941 acatccgcaa ttcgtggtcc cagttacgtt ttgcggtggc gacgatcaag tcatcgaacg
     6001 ggctctcata ggtctttgcc caggcatcga attcgtcgtg tagtcgagtc ggcaggtacg
     6061 gcttgtagtc gtacagatcg gccccggcgt gggtgtccgt tgaaataacg acgtagcgat
     6121 cgatcgtgtc ccaagtagca gccattgcct catccttcta ccgagagagg cgggcgcagg
     6181 caggattgcc cagtttggcg tgcggaggcg ttacgacgaa acgaccccgc tgtgcgatga
     6241 gatttcggtc ggcagctcgc cgaccacgtc gaggctgaga aactccggcg ccttgatgac
     6301 ccggcgaagc atgaaccgaa tgaacgccgg catgtccgag gcgttctcat cgcggtacag
     6361 ccggggggcg ccaagctgat agtgccgccg ccggcttacg agttcgcagc cgccagccga
     6421 cagaacattg cgcacccaat ccgcactgga tccgtacgtc agcgcgatca cgtatccgcc
     6481 gcggcgccga aatgcccata acggggtgcg caacgaccga cccgatttgc ggccccggtg
     6541 gatgacgacc gcccaccctg gcgcccatgg agcgatgaac ttcgtgagcc ggttgagccc
     6601 aaccttgttg gcgcgggcaa cccatcgtgg tgctggcatg tccaaaactc ctcacttgtg
     6661 gaatttctag acacgacgct aactcgaggc cgtccgaaag tcaacgactg tagaatttct
     6721 ggcactatgg ccaggatcgg gcgaccgcgc ggtaagaccg acacccgcaa cgtgatcctg
     6781 gcggccgcgc ggcatctttt cgccgacgcc ggttacgaca agacttcggt gcgcgacgtc
     6841 gccaccgcgg ccggggtcga tcccgcgatg atccggcact atttcggcag caaggccgaa
     6901 ctttttcgcg cgacgatggg atggccgttc gagcccgctg agatcgcagc tcgcgtcacc
     6961 ggtggcggtc ggggcgagat cggcgcgcgg ctcacgcggg tgttcttcga agcgtgggag
     7021 caaccgaatt cgcgtgcgcc gctgctggcg atacttcgcg gcgcggccac ccacgacgag
     7081 tcggcgaacc tggtgcgcca gttcattcag ggccaggttt actcgcacat cgcggcggcc
     7141 ctcgcagagc cggatgccga gctgcggatc gatctggcga tggcgcagct gctgggcatc
     7201 gcctacctgc ggcacatcct gcgggtggaa cccgttgcgt cgacgccggt ggaggatctc
     7261 atccaacggg tcgcaccggc aatcacccgg catctgacgc cgccataatg agccccgaaa
     7321 ctgctgggag atcgcgtttt tcggcgcgag gacgatctgg ccgcagtttc ggcgcagtca
     7381 ttccccgtgg atatcgaggc cgtgcgcggc ggcgaaggcc atggcctgct cgatgtcgac
     7441 cttcgcgccg cgcaaggagg cggtcgtcca cagcgtcgga tccacccgcg cgccgcgcag
     7501 atccgcctcg tccagcttgg cgttctgcag ccgggcgccg ctgaggtcgg cgcgtcgcag
     7561 caccgccttg cgcaggtcgg cctccaccag gctggcttcc cgcagtcggc agtcggacag
     7621 gtcgacagcg cgcagatccg acgcaccgag cacggtcagc gtgaggtcca cctccacgag
     7681 cttgatcggc cggatgcggc attcggtgaa caccgaacct aggaagctgc agtgccgaaa
     7741 cgtgctgtgc aacagagaag tccggcgaaa ggtgcaattg cgaaacgcgg agccgacatg
     7801 ctcggattcg gacatgtcga caccgctgaa gtcgcactcg gtgaatacga cacgctcggt
     7861 gcgaagctgg ctcagatcgt cgtcgcggaa gtcgtggccg tcgaattccc gctcgaccca
     7921 gaccgtttcg ttgtcaccca cgtaccggcg tgagcgtgtt gagtgcgtat tcggtgacgg
     7981 cgatcagcgc cgccttcgtc gaatggcggt cgcgcgcgtc gaccgtgatg accgggatgt
     8041 cctcacgtag cgccagcgcc ttgcgcaccg catgagcggg atgtcttggt gcgccgtcga
     8101 attcgttgac ggcgacgagg aagggcaggt tgcgcgcttc gaaaaagtcg acggcggcaa
     8161 agctgtcctg cagtctgcgg acgtcgacaa ggatgatcgc gccgatggcg ccgaggacca
     8221 ggtcgtccca catgaaccag aaccggcgct ggcccggcgt gccgaacaga tacagcacca
     8281 ggtcatcggc cagtgtgatg cggccgaagt ccatcgcgac ggtggtggtg ttcttctgtg
     8341 gggtggcctc gagaccgtcg accccttccg actgttgcgt caccaacgct tcggtacgca
     8401 gcggcatgat ttccgacacc gccccaacga acgtcgtctt accggcgccg aagccaccgg
     8461 agatgacgat cttcgtcgag gcggtgttcc gcctctcaga gcgctcgtag gccacgcagc
     8521 gtccttccta tcaattcgcg acgttcgtca acggtcgtgg agtcaccgag ggtggtgtgc
     8581 acccgcagat aaccttgcgt cacaaggtcg ccgatcagca cgcgcgccac gccgaggggc
     8641 aaggataaga ctgcggcgat ctcagcgacc gacgggctct cggcacaccg cgtcaggatc
     8701 tggccgcgca cgtcgtttcc cggccagcgc ggcggtttcg gtgggtccgc caccgtctcg
     8761 atcggtgcct ccagcggcaa gtagatgcgg gaatcggtcc ggccggccgt cagggtatac
     8821 ggccgaacca gctggggttc gtccgtatcg tcccgttccc aatggtccat aggcggctca
     8881 cgagtgctgc ggggcgcgtc gtgccgcttg ggccatcgcg cccactcgct ccaccaaaat
     8941 ggccatctcg taaccgatct ggccaatgtc gcagttgcgg gtggccagag tcgccaggtt
     9001 ggagccgtcg ccgacacgca tcagcagcag atagccgttc tccatctcga cgaccgactg
     9061 cagcacgtag ccgccgtcga acagctgcga ggcacccgtc gacaggctcg ccaggccgga
     9121 ggccaccgcg gcgagctggt cggcgcgttc ggtcggcatg tgctcactgg ccgccatcag
     9181 caggccgtcg gcggacacca agacggcgtg cgagacaccg gatacttcgc gggcgaactt
     9241 ggagaccagc cagtcgagcg aatcgcgctg cgtcgaacgt ggcggatagg tcattcgtta
     9301 tcggttcctc ttgtttcttg agcgtgtgat cgcccggcat gcacgccacc gaaatggttg
     9361 ctgatgcttg cacggacggc gtccggatcg cgtggcggaa tcggtcctcc accagtgccg
     9421 aacccagccg ccgatgctac ctcgtcggcg tcgtcgtcgc tcctgtgagc accaccgttg
     9481 aagtggtggc cgccgttgga gggggcgtcg gggaccgcgg aatcgaccgc acccggcacc
     9541 aaccgcgcac cgggctcacg caccggcagg ccttcctcgg tgtgctcggt aaccggcgcc
     9601 tcgtctgcgg ccgcggctgc cgaccagccg tgatcccaca ccgattccca gttcaggtcg
     9661 tcgctcttgg cgatctcggt ggggtcgacg agccattcgg acagcatctt ctgatagatc
     9721 gcgtcatcgg cgcctgccgt cggtgacgat tcgcgcacag cgtggcgcga cggctcgggc
     9781 tgcggctgct cggccacggc cggcggctcg tgttgcgagg gcgcctccgg ccgcggctgc
     9841 tcgcccctcg gcggggtggg cacgccgttg ctgacggcct gcgcgcggga tgcgaagaag
     9901 gccgacgtgt cgatcggagc gcggtcggct tcgggttcct gggcggcggg ctgctcccaa
     9961 ggatttggct cagctgtgtg caccggcatc gagtcttccg gccacagctc ctcagcgggc
    10021 cactcgtcgt cccctttctc cggctgagcc agcgaggccg ggacgtcggc aattccactg
    10081 gcgcccggat tgcgctgcgg caacaactcg atcggcactt cggggtggcc gttggtgtac
    10141 tcgtggccgg gctcgtcgaa cgtgtactcg tgttcctcgt ccagggccag ggcggtgtgg
    10201 atgcccgcgt gcgcgtcggc gggtacggcg tactcggggc caccgaactg gtcgggcgcc
    10261 ccggccacgg ccagcaactc ggacggcacg aacacgccag cggtggtgcc cgaattcggt
    10321 tcacccgcaa tggtgctttg caaccggacc accagcccgt gctgcttggc gagccgcccg
    10381 accacgaaca ggcccatgtg ccgcgcggtg taggggttga cctcgccgcc cgactgcaga
    10441 cgggtgttgg ccacccgcag atccggttcg gtcatgccga tgccgatatc gctgacctcg
    10501 atgaccaagc cgccgttgcc ggtgtgcacc gccgacaccc ggacttggga gatcggcggc
    10561 gagtagcgca gggcgttgtc gagcagttcg gcgagcaggt gaacaaggtc gccggcgagg
    10621 gcgccgacca cttcgctgtc gggcaccgtc gcggtgacca cccgggtgta gtcctccacc
    10681 tcggaggctg cggcgttgat gatcgccgac accggcaccg ggtcggcctg ctcgcggggc
    10741 accttggcgc cggccagcac caacaggttg gctccgttgc ggcgcatccg ggcggcgagg
    10801 tgatcgaggc ggaacaggct ttccagccgc tcgggatcct cctcgttgcg ctcgaggcgg
    10861 tcgatcagcg agagctgctg gtccaccagc gaccggctgc gccgcgacag cgtctcgaac
    10921 atgtcgctca cctgcagctg cagccgggac tgctcgccgg ccagcaggac ggcctgctcg
    10981 tgcagctcgt cgacggcgtg cgcgacctgt ccgacctctt cggaggtgta aacggggatc
    11041 ggctggacgt gcccgggctc accacccgca cggacccggt cgagctcgcg ggcgaggtcg
    11101 tcgtgcgcca cctggagcgc gctgtcgcgc agttggcgta gcggacgcac cagcgagcgc
    11161 gccaccagga tcaccagcag cagcgccagc agcatcgcgc cgaggacgat cgcggagtcg
    11221 cggatcgcgc cggtgcgctg cgcggcggtt tccctctcca ctgcgcctgt caccgcgctc
    11281 gacgtgtcgt cgatgatctt ggtcgcgatc tggtcggtga cctggatcga tgcattcagg
    11341 tccgggttgt tgaccagcac ggctgccggg tcggacatga tcgtcatccg cttgacgaac
    11401 tcttcttgca gtttctgagc ctcgggtgat ccgactccca ggacctggct catgccgaac
    11461 agcgtggacg gctcggtgcc ggccaccgtg ttcatcgaca tgcgcagctc gggatcgggc
    11521 agctcaccgc cgaggctgac cagcagctgc tgaatcatca tctggccgcg ggcgccgacg
    11581 gcccggctca ggcccagcgt ctcggcgcgg atgcgttcgt cgtcaacccg aaccgagccg
    11641 ttgatcgcgt cctcggccgt cagcaggatc ggcgtgtacg cctgcacgcg gtcccgcagc
    11701 ccgacggagt tggccgtgac cttgtcgatc agtccctggc ccgcgccgat catcgtgctg
    11761 acgcccttgc tgacgtccgg cgcgatgtcg gtgtccgtca gccgtcgctg caattcctgc
    11821 ctgctggcgt cgaacttgga gagcgccgcc tgggtgtctc ccccggtcga actcgccagc
    11881 attgcggcgt cgagtgacgc catgtagttc acgatcgcgg gcaccatttc ggcgcgatcg
    11941 gcggcgcgcc gcaaatcggt tgcttccgtc gcgctggagt agatgcgcaa tccgccgaac
    12001 gtcccggcga gaaccaacgg caccaaaacg atcgcgaaca ctttccagcg aaccggccaa
    12061 tttgcgatcg accatcgcga gggtcgcttg gcaggtggct gcttctttgg ttggaaatcg
    12121 acgagcgtgc cgtcggcctg cttcagctgc gagaacgcgg tcatcggcgt ccgccgctac
    12181 tatcggccgc ctgctcgcaa ttcagtaatg aagcgtcgtt cataagactt cctgctgatt
    12241 tcgccctcca cgggcgggcg ttctacgcct ggcaattggt cgagtatgcc agccatgtgc
    12301 agctgtttcc acaattctta ctgaacagac agagttcata cccgctccga caagctgttt
    12361 cgtgctatcc gagcaaattt ccgccagcgt cgacgcatta ctgaggcgtg ccaaacgtcg
    12421 gccaagcgca cggtcgctgt gttcgaatta accaacgcca cgatgagagg ggacatcatg
    12481 accgagcccg agaagtttga ggtagatccg tcgggtgacc cgaatcccgg cgtagccgac
    12541 catgcgttgc cggatgacgc caaagggcct catccgctgg ctgatcctgc caacgatccc
    12601 actcccggtg tgcccggcca cgcaaaggac gactgaggct ttgggcccaa accccgccga
    12661 atgagttcaa gttgatgtct actcgccgcg gatcgacatg cgccaaacag gacttggccc
    12721 gcaacccggc acggcttgac taggggaacc ctgtctacat tccgggtggt ccagcccaaa
    12781 agtcttcggc caggccgcga cgagggtcct cgcctgccgc ccgatcctcc tcggcccact
    12841 caccacgact gggccacgct tcgcgcagaa cgatcaccag caccacgatg cccgcagcga
    12901 tgatcaccgc cacgatcacc agcgcgacca caaccacggc cgacatgagc tgaggctacg
    12961 cccgtcggtc gcacaaaacc ctctctcgac ctcaacagac gtagcctgcc caagacgcgc
    13021 ttgctgagag gatgccgtga tgtttcggct gatgttctat tcgccgcgga tcgcgcccaa
    13081 cacaggcaat gccatccgga tggtggccgc aacgggatgc gaattgcatc tggtcgagcc
    13141 gctgggattc gacctgtccg aaccgaagtt gcgccgcgcc ggcctggact atcacgacct
    13201 ggcgtcggta agcgtgcacc gcgacctgcc cgccgcctgg gaggccgtta agccaaagcg
    13261 ggtgtacgcg ttcaccgcgc atgccacgac gtcgttcgcc gacgtcagct accaaccggg
    13321 cgacgtgttg atgttcggtc ctgaacccac cgggcttgat gcagcgacgc tcgccgaccc
    13381 acacatcagc gcgcaagtgc gcatcccgat gcttgtgggt cgacggtcac tgaatctgtc
    13441 gaacgccgca gccgtcgccg tctatgaggc ctggcgccag aacgggttta ccggggcggt
    13501 gtgactacca cgtgtcccag tgcgtgacct gctcggcggg cagccggtca gcccgacgga
    13561 agtcggtgcc cttcgtatag gcgatcggga agaggccggc ctggctgtac tggtcgaacg
    13621 ggatgccgag caattcggcg gcctgcttct cgccgtcgcc gagcagatgc agcgttgtcc
    13681 atgccgatcc aagtccccgc gaccgcagtg ccagcatgaa gctccacacc gccggaagca
    13741 gcgatcccca gaacgacgcg ctcatgcccg ccggtgcgcc gtccggacgt ccctccaagc
    13801 acggaatcag catgaccggc gcctcgtgaa ggtgttcgtt gaggtacttt gcggagccgt
    13861 acacgcgggg ccgctgctcg tcgcgcacat cgccgtactc gggcttggga aggtctaggt
    13921 acggcgtcgc attcgtgctg tagatgtcgg cgagcgcctt cttcttttcg ggatcgtcga
    13981 cgaacaccca ttgccagccc tgggcgttgg agcccgtcgg cgcctgaagc gccaagtcca
    14041 gacactcggt cagcacctca cgcggcaccg gcttctcgaa atccagccgc ttgcgcaccg
    14101 agcgtgtggt ggtcagcact tcatcgacgg acaagttcaa ggtcattttc cgagactaca
    14161 ttcgtcgtta tgacagtcga actgacgcaa gaggtttccg acaggctctc atccgatccc
    14221 tacggctggt tgacgacggt ggcgaagtcc ggccagcccg tcccccggct ggtgtggttc
    14281 tacttcgacg gcaccgacgt catcgtctac tccgaaccca acgccgcgaa ggtccgacac
    14341 atcaaggccc atccgcgggt gagcctgaat cttgactccg acggcaacgg cgccgggatc
    14401 atcatcgtcg gcggcaatgc catggtcgac gccgacggcg ccgatccgtt gcaggatgag
    14461 cagtatcggg cgaagtacgg cgagcttgcc gccagcttcg gcttcagcga ggaattcctc
    14521 gccgcgtaca acacccggct gaagatcagc atcgacaagg tctggacgac accgaccgag
    14581 ggttagtcgg ctcagcggaa gtcgcggctc tttgagccaa ctgacatgga gagcttgccg
    14641 attcggtcgg cgaccacggt gaccgcaccg gtggcgttct gcacctgacc gcggatcagt
    14701 agggcggacg ccgtctgcgc cagcctgcgg tgtcgcgccc ataccccggg cgcgcacagg
    14761 acgttgacca tcccggtttc gtcttcgagg ttgatgaacg tgacgccctg cgcggtagcg
    14821 ggccgctgtc gatgcgttac cgccccggcc accagcaccc gagttccgtc gggcaccgct
    14881 agcagctggt cagcgggcac gacgccgatg gcctcgagat cctcccgcag gaactgcgtg
    14941 ggataggtgt ccggtgaaat accggttgcc cacacatccg cggctgccag ctccagctct
    15001 gtcatgccgg gcaaagacgg gatgtgcgac gacgacccaa cgcccggcaa ccggtcgggt
    15061 cgctgggtgg cggccgcgcc ggccgcccac agtccttcgc ggcgggtgat cccgaagcag
    15121 cccagcgccc ccgcggtggc cagcgcctcg gtctgcggca cggacagctg gatgcgtccg
    15181 gtcagatcga gcagagaatc gaacggcccg ttggcctttc ggtcttcgac gatctgctcg
    15241 gccaactcgt cgccgatgtg gcggaccgct cccaagccca gccgcacctc gaggccttcg
    15301 ttctccagcg tggcatgcgg aaggcttgcg ttgacgtcgg gtccgtgcac tgtcaccccg
    15361 tgcctgcggg cgtcggccac cagcgactgc ggcgagtaga agcccatcgg ctgcgcgcgt
    15421 agcagtgccg cacagaacgc cgcaggatga tgcagcttga accacgacga gtagaacacc
    15481 agcgcggcaa agctcaatga atggctttcc gggaaaccga aattggcgaa ggcctccagc
    15541 ttctcgtaga tccggtcggc cacatcgccg gtgatgccgt gccgctcacg catgccgtcg
    15601 tagaacctgc cccgcaatcg gcgcatccgc tccgtggagc gtttggatcc cattgcccgg
    15661 cgcagctggt cggcctcggc ggcggagaag ccggcgcagt cgaccgccag ctgcatgagc
    15721 tgttcctgaa acagcggaac ccccaatgtc tttcgcagtg cagcagccat cgaccgatgg
    15781 tcgtaggtga caggttcgac gccgttgcgc cgcttgatgt aggggtgcac cgacccgccc
    15841 tggatcggcc ccgggcggat cagcgcgacc tcgaccacca ggtcgtagaa cacccgcggc
    15901 ttgagccgcg gcagcgtggc catctgcgca cgcgactcca cctggaacac cccgacggag
    15961 tcagccttct gcagcatttc gtacaccgcg ggctcggaca gatccagttt cgccaggtcg
    16021 acgtcgatgc ccttgtgctc gcgcaccagg tcgatgcagt agtgcagcgc cgagagcatg
    16081 ccaaggccga gcaggtcgaa cttcaccaaa ccgattgccg cacagtcgtc tttgtcccac
    16141 tgcagcacgc tgcggttctc cattcgcgcc cactccaccg ggcatacatc ggcgatcggg
    16201 cggtcgcaga tcaccatgcc gcctgaatga atgcccatgt gccgaggcaa gttggagatc
    16261 tgcaacgcca ggtcgatcac cgactcggga acgtcttcga catgagtcga ttcggacaag
    16321 ttcccccact gaccgatctg cttgctccaa gcgtcctgct gcccctgcga gaaccccagc
    16381 gcgcgggcca tgtcgcgcac cgcgctgcgg ccgcggtagg tgatcacgtt ggcgacctgg
    16441 gcggcgtagt cacggccgta gcggtcatag acgtactgaa tcgcgttctc gcgcaagtcg
    16501 gattcgatgt cgatgtcgat gtcgggcggc ccgtcgcgcg ccggggacag gaaccgctcg
    16561 aacagcaact cgttggcgat cgggtcgacg ttggtgacgc cgagcgcata gcagaccgcc
    16621 gagttggccg ccgagccccg cccctgggcc aggatgttgt tatccctgca gaaccgggtg
    16681 atgtcgtgca ccaccaggaa gtagcccgga aaggtcagct gctcaatgat tttcagttca
    16741 tgttcgatct gcgcgtaggc ccgtggcgcc cgctccggcg ggccgtacct cccgcgggcg
    16801 ccctccatga ccagatggcg cagccagctg tcctcggtat gtccctccgg aacgtcgaac
    16861 ggcggcagct gcggggcgat cagcgccagc ccgaacgcgc actgctcgcc gagctcggcc
    16921 gcggccgtca ccgcctcggg ggcccccctg aacagccgcg ccatctcctc ccccgagcgc
    16981 agatgcgaac cacccagtgg cgcaaggtaa ccggccgcct cgtcgatcga attgcgggcc
    17041 cggatcgcac ccatggccat ggccagccga ccccgcgacg gtgcggcgaa gtgggctgcg
    17101 gtggtggcga cgacattgac accgaaccgc ggcgccagct ccgccagcgc aacgttgcgc
    17161 tcgtcgtcga ggggatgacc atgatgggtc agctcgatgc tgacccggcc ggcaccgaac
    17221 cggtccacca ggtcggcaag cgccctgccg gcagcctccg ggccacccgt cgataatgct
    17281 tgccgtacat gacctttgcg acaaccggtc aagatgtgcc agtgtccgcc ggccgcctcg
    17341 gtcagcgcgt cgtagtcata gcgcagcttg cctttctcgc cgcctgccag atgcgcggtc
    17401 gccagctgtc gcgacagccg tcgatacccc tccggccctc tggccagcac cagcaggtgc
    17461 ggtccgggcg gatcgggatc ctcggtacgg tcaccaccgc ccagtgacaa ctccgcaccg
    17521 aacaccgtct gcatctcgag ttctttggcg gcctccgcga accgcaccac cccgtagaga
    17581 ccatcgtggt cggtgagcgc gatggcccgc agatcgagcc tggccgcctc ctcgacgagt
    17641 tcctccggag tactggcgcc gtcgaggaag ctgtacgccg aatgcgcgtg caactcggca
    17701 tacgggaccg acgagacagg gcgctccaca tcgagcggct gatactcccc tcgcttgcgc
    17761 gaccaggccg gactgtcgcc accgtcaccc acttgctggt ctacaggcca tccggcacgg
    17821 cgcggtttgc cgttgagcac ccgctccatt tccgtccagc tcggcggccc ggtgaaccag
    17881 ctcaccgatc cagtctatcg aacatatgtt ctagtagcca gaccgatgat ttccgccttc
    17941 gcgtccggtc aacacggcat gggaacgaag cgttcgcgca gcgggcatct gccgatcaac
    18001 ggcctgaacc tctaccacga ggtgtatggc gagctcggca agtcgaaagt gcctccgttg
    18061 ctgctcattc ctggcgcgtt catggccacc gattcgatga agtcgtgggt gtccgcgttc
    18121 gcgggcaaac gcgccgtgat cgtcttcgat cagcaagggc acggccgcac cccggatacc
    18181 tcgcgcaaga tgtcgtacga gcagttcgcc gacgatgccg cggagttgtt gcgcgccttg
    18241 aaggtcgagc gcgcggatgt gatgggctac tcgcaaggcg gcggggtcgc gctgcagctc
    18301 gcgctccgtc acccgacgct cgtcgacaag ctggtatcgc tgtccgccac ctaccgccag
    18361 gacggctggt acccgtcggt gttgaagggc attgagggtc tcaccgccga agcgtttgcc
    18421 ggcacgcccg tcgagaaagc gttcaaggag cacacgccgg acgccaaggc gtacggcgcc
    18481 tatctgaaaa agatgaaggt cttgaacatc caggaccaga acatcagcga cgcgcagatg
    18541 cgttcgatgt ctgcgaagac catggtcatt gtcggtgatg cggatggcgt gaaaccggag
    18601 cacgcagtag cgatgttcaa gctgcgcggc ggcggcgacg aggaagcggc ggcgactggg
    18661 acgttgcaga aggttccggc cgcgcgcttg gtgattctgc cggcgatgtc gcacattggc
    18721 atctctgcgg agacagcggt gctggtaccg atggtcagtg cgtttctcga cgatgtgccg
    18781 ccgacgacgc cggatctctt ctaggcgcga tgatttccac gcccgcggcc ggtcaatact
    18841 tcagacgaca attcaagcct tggaggaacc atgaccaagc agaccgccac cgccctaccg
    18901 ccggtggtcg accaagacac ctggcgcgcc gcactcgatg acctgcgtcg acgggagaaa
    18961 gccgccaccc gcgaactgga tgccattgcc gcccagcggc gccgtctgcc catggtcgag
    19021 atgccggatt acacgctgat cggcaaggat gggacggttc ggttggccga cgtgttcgac
    19081 ggacattcgc agctcatcgt ctacaaccac atgtgggagg acggagccga gtggcagtgc
    19141 ggcggatgca ccggcttgac ggcagggtgg acccgactgg agttcctcgc caattacgac
    19201 gcacgcatgg tcatcgtcac caacgggccc atcgaagaag ccttggcata taagcagaag
    19261 gtcggcaaca agatggagtg gtactcgtca tcggagagtt cgttcggcgc cgacgtcgac
    19321 gcgccgccgg gtggcggctt cgccgtcaac gtattcctcc gcgacggcga caaggtctac
    19381 cgcacttggc acaccaacgg ccgcggcacc gagcaactca cctacacttt cggcctcatc
    19441 gacctgctgc cgtgggggcg gcaggaagaa tggcaggact cccccgacgg ttggccatcg
    19501 cgaccgacct actccggctg gttggactcc cctgacatcg cgcgagtgta cggacggacc
    19561 ggcggctaag gcaggtcctg cgccccgaat tggtctcgcg gcacgaccat cattggagtg
    19621 tccagcaccc gcagcatctt ggcggcggtc gagccgagga agaggcgctt gggcgcactg
    19681 agccggctcg acccgaccat gatcaggtcg ccgtcgtgcc actccagttt gcgcaccgcc
    19741 tcctcgaccg tcgctccctc gacggccgtc gctgcgactg ggaagtcttc cggcagggag
    19801 cttttcgcgg tgtccagcac ctgctgggca tgcgtcagtg cacgctcgcg aaccgcttcc
    19861 tcgtcgccgc gcaactggcc gaatatcgga tcgagcgcca ccagcgaaac caaccgcagc
    19921 ggtgtaccgg ccgccttgct gaaccgaacc gctttctcca acaacagatc cgcgccctgg
    19981 cgctgtccga gtgcgcaggt gacctcacgg acccgatcga tcttggtatc acgggtgccg
    20041 cgtgcggcga ccacaacggg caggggcgcc gagtgcaaca gttcgttgac cacagagccc
    20101 agcgagtaag gaccggccag cccgccgccc gatccgccca ccacgatcag gtcggcctcg
    20161 agccgcgcgg cctcccggat cagcccatcg gcaaatgagt cgtcgaaact cacgtggccg
    20221 tgcacggtga ggtcgtcggg caccgacgcg atcgcctcgg cgatccactt ctcggcctgc
    20281 tcacccagtt tcgtctcgta atcacgcgta ctcgctgccg gcagcacccg gtcgggcggc
    20341 aggatgatgc acgcctcgac gtcggcgccg agcgttcggg ccagccgggc agcgagtgcc
    20401 atcgcgtctg cgccacccgg cgtcgccaga tatccaacga ccaacttcat agccaactca
    20461 ccgccctctc gcaccacacc gtgcgctgtg gcccatcctg tcaagatgcg gcggccgtta
    20521 ggccactttg gcgatgaccg gctcaggcgg cgtactcggc ggtgccgtcc tcgagcacca
    20581 acctggcgtc ggagccgtcc gggttggacg cctcggtgcg gaacacggcg tgccccgact
    20641 cggtgcgcca gatcgacgtg gtgagcgtct cgcccgggaa caccggtgag gtgaaccgcg
    20701 cgccgacttg gctgaccttg gtggcgtcgc cgtcgccaag ctcgctcacc agcgcgcgac
    20761 cggcgactcc atatgtgcag agcccgtgca ggattggctt cggaaacccg gccaagttct
    20821 gcgcgaacca gggatcgctg tgcagcggat tgcgatcacc ggacaaccgg tagatcagcg
    20881 cctggtcttc acgcgtcggc agcgcgatgc gggcatcggg ctcgcggtcg ggaatctccg
    20941 gggcgacggg ccgctgtccc ggctgcccgc cgaagccgcc ctcgccgcga atcaccgccg
    21001 tagtcaacgt ttcagcgatg acctccgatg tgtcggggtc ggtgccggtc gccttcaaca
    21061 tgacgatcgc attcttgccc tcacccttgt cctgaatgtc ggcgacctcg gcgacgacgc
    21121 tgagcttgcc ggccggcggc aagggcttga acaaccggat ctcctgcgat ccgtggagca
    21181 gcatcgcgaa gttgaaggag ccgatcttgg ccgccgcggc gaacggaagg caggcgatga
    21241 cggcgtaggt cggcagcacc tgctgcgggg tgtcgtggct gttctcggtg gtgaacgcga
    21301 ggtcgtcgat gcctgcaccg acaccgagcg catagagcag cgtgtcgcgg tcggtccatt
    21361 tgaagagttg ggcgtctgtt ttcgcgccta tggcgttcgg gtcgatcggc atagtcggac
    21421 gatatcgggt cgatccggcc gcagtcgacg accacgtcac acgttgttga cctttgcacg
    21481 gcaacgggtg accatggctc gcatgcgcta tgtcgttacc ggcggtaccg ggtttattgg
    21541 ccggcgggtg gtgtcgcaga ttttgggccg tcacgctgac gccgaggtgt gggtgctggt
    21601 ccgacggggc tcgctgaccc gcttcgagcg gctcgccggc gagtgggggc cacgggcaaa
    21661 agccctggtc ggcgacctca ctgcgtcgag cctcggcctg accgacgacg cggtcgccga
    21721 gctgggcacc gtcgaccacg tcgtccattg cgcggcgatc tacgacatca ccgccaagga
    21781 ggccgaccag caggcggcca acgtggaggg cacccgagcc gtcatcgacc tggcccgtcg
    21841 gctggacgcc accctgcacc atgtgtcgtc gatcgccgtc gccggcacgt atcacggcga
    21901 gttcaccgag gacgacttcg acgtcgggca ggatctgccg acgccgtatc accagacgaa
    21961 gttcgaggcg gagatgttgg tccgttcggc ggcgggactg cgctaccgcg tgtaccggcc
    22021 cgcggtcgtg gtcggcgatt cccgcaccgg agagatggac aaggtcgacg gcccctacta
    22081 cttcttcggt ctgctggcga agctggccct gttgccgagg ttcaccccga tggtgttgcc
    22141 ggacaccggc cgcacgaaca tcgttccggt cgacttcgtg gtggacgcgt tggtcgagct
    22201 gatgcacgcc gacggtcgcg atgggcagac cttccacctc accgccccga aaaccattgg
    22261 gctgcgcggc atctaccgag gagtcgccga agcggcaggg ttgccgccgc tacgcgggtc
    22321 gctgcccggt gcaaccgcga agccgttcct ccgtgtgacg ggacgcgcca aggtgctgcg
    22381 caacatggcc gccacccagc tcggcatacc gggtgaaatc ctcgacgtcg tcgaccttgc
    22441 gccgaccttc acgtcggtca atacgacaga agcgttgcgc ggcaccggga taacggtgcc
    22501 cgagttcgcg tcatatgcgc cgaggctgtg gcggtattgg gccgagcacc ttgaccccga
    22561 tcgggcccgc cgcgatgatc cagctggtcc gctggtcggc aagcatgtga tcatcaccgg
    22621 cgcgtcgagt ggcatcggcc gcgcctcggc gatcgcggtt gccgaacgcg gcgcgacggt
    22681 attcgcgttg gcgcgcaacg ccgaagcgct tgacgacctc gtcgccgaga tccgggcagt
    22741 cggcgggcag gcacacgcgt tcacatgtga cgttacggat gcgacgtcgg tcgagcacac
    22801 cgtcaaggac atcctgggcc ggttcgggca catcgactat ttggtgaaca atgcgggaag
    22861 gtccatccgg cgctcggtga tcgcgtccac cgaccggcta cacgactacg aacgcgtgat
    22921 ggcggtgaac tacttcggcg cggtccggat ggtgctggcg ctgctgccgc attggcgcga
    22981 acggcggttt ggacatgtgg tgaacgtgtc cagcgcaggc gtgcaggcgc gcaacccgaa
    23041 atacagctcg tatctgccga cgaaggccgc gctggatgcg ttcgccgatg tggtgtcgac
    23101 cgagacgctg tcggaccaca tcacgttcac caatatccat atgccgctgg tgaagacgcc
    23161 gatgatcgcg ccgtcgcgcc gtctcaaccc ggtaccgccg atcagccccg aacacgccgc
    23221 ggccatggtg gtgcgtggcc tggtggaaaa gccggcgcgg atcgataccc cgctcggcac
    23281 gctcgccgac ctcggcaact atctgacgcc gaagctgtca cggcgtgttc tgcaccagct
    23341 ctacctcggc taccccgatt cggcggccgc ccgggggttg gtgcctgagc cgtcgacggc
    23401 acctgccccg tcgcggcgac ccaggagtcc ggcgcagcgg gtcacgccgt tgagggtgcc
    23461 gggaccgatc aagcgtgccg tgcggctagt gccaggcgtg cactggtagc ggcatcaggc
    23521 cgacgaggcg gtctcccaca ggcccatgat gttgccctcg gtgtctttga aatacgcggc
    23581 gaatcccatt tcaccgacgg gcgtcttctc gccaacaggc tcaccaccga gcgatacgat
    23641 cttcgcgagc gcgtcatcga tgctgtccac atcgatcgtg atcaccgggc cctgcggaaa
    23701 ggcaggctcg cgttcgaaca tgcctccgcc gatgtatccg ggttcggccg gcatccccga
    23761 ttcggctgac gggccggtcg agacgatcgt gtacctcatc tcgggcatct cgttgagttg
    23821 ccaaccgaag atctgtttgt agaagtttct agcgcggtca ccgtcactga agggcacctc
    23881 gaaatgcacc acacgaccag tcatttccgt ctcctcgtgg cagcggtctg agtaatcggt
    23941 tgcgagtcac agtctaggcg tgcggtgacc gtgccacggc cgtatcgtga cgggatgacc
    24001 gtggggggtg aggggcctgg cggcccgcta cccgtcttcg ttgatgtcga tacgggcgtc
    24061 gacgatgcga tggcgctcgt gtacctgttt gccagcgagg acgccgaagt tgttggcatc
    24121 gcgtcgacat cgggaaacgt tccggttcaa caggtttgcc ggaataacct cgggttgctg
    24181 gacctgtgtc ggatcaccgg tgtgccggtg tcgaagggcg ctgagcagcc gatcagcacg
    24241 ccgctgcgca ccgctgagga cacccacggc cccgagggct tgggctatgc cgagttgccg
    24301 ccgaccgatg gtcaactcac cgcccacgac gccgccgagg cgtgggtgcg tgccgtgcac
    24361 gcctaccccg gtgaactggt ggcgatcgcc accgggccgc tgaccaacct tgcgctggcg
    24421 ctgcgcctcg aaccggcact gcccaagctg ctgcggcggc tggtgatcat ggggggcgcg
    24481 ttcgactatc gcggcaacac cacgccggtg gccgagtgga atatcagcgt cgaccctgag
    24541 gcggcggctg aggtgtttgc cgtgtggggc gcagcgtggg gactggagcc gccaaagcac
    24601 atcccaattg tgttgggact caacctgacc gagaacgtcg ccatgacgcc gacaatcttg
    24661 agccggctcg cgacggccgc ggagtcctca tcggtagcga tgagcgtgct cgacgaccgc
    24721 gggacgcggt cggtggcatc caatccgctg atccgcgtgc tcgaggacgc tatgcggttc
    24781 tacttcgagt tccactttga tcagggcgag ggctacctcg cacatctgca cgacccgctg
    24841 tctgcggccg tcgccctgga ccccgacctg gtgcagtacc gccccgccgc gattgacatc
    24901 gagttgaccg gcacgctgac ccgcggcatg acggtcgcgg actggaatag ccactgggga
    24961 cgagaaccca acgcgctcat cggcattgac gtcgatcccg gcgtgttctt cgatcggttc
    25021 atcgccaggg tcgggcggtt cgcgcagcga ctgggctgaa cgtcgaaact gccgggagat
    25081 cgcgaaattt gccgagatcg cgatctgccc gcagtttcga aaggactcag tcgtcggtga
    25141 cgacgagctt gacgtcgatg ttgccgcgag tggcgtttga gtaggggcac acctggtgcg
    25201 ccttctccgt cagctgctgc gcggcgtcgt ggtccaggtt gggcagcgtc acctcgagtt
    25261 ctaccgcgag accgaagccg ccgttgtcga tctggccgag cgaaacccgc gctccgacag
    25321 ctgaatccga gacgtcggca ttctcctggc ggccgaccaa ccgcaacgcg gagtgaaagc
    25381 aggcggcata gccgacggcg aacagctgct cgggattggt gccgttgccg ctgccaccca
    25441 tctccttcgg aatggcaagg tcgagatcga gcttgccgtc ggaggtgcgg ccgtgaccgt
    25501 cacgcccttc gccggtggtc agggcttcgg cggtgtagag ggtcttcatg cggattcctt
    25561 tcgttgcgat gcattgagtg catcagtgag gtgacggacc gcgtcgcgca ggtccgcggc
    25621 ttcttgttcg gtcattccgg tcgagctgaa tagttgttcc gggatgcact gggccttgcg
    25681 ctcgaggcga cgccctgcgg gggtgagcgt cacgtcgacc agccgttcgt cgtccagcga
    25741 cctctcgcga cgaacaaacc cgttggcctc caaccgcttc agcagtggcg agagcgttcc
    25801 ggagtcgagc tgcagccgct caccgagccg gccgacggta acgcgatcct cttcccacag
    25861 caccagcagc acaaggtact gcggataggt gaggttcagt tccgtgagga tcgggcggta
    25921 cgcggccgtc atcgcccggg acgccgagta cagggcgaaa cagagctggc ggtcgaggct
    25981 caacatggcc atgccagaac tatagctcac aactcagttg tgcacaactg acctgctagc
    26041 gggtggcgat gtagtcgcgg aacgcctgcg gtacctggga tgcgtcgagc ttcgtgaaga
    26101 agtcgggtgt cgtgatagcg gaatacgact cttcgccctt gatgaaacct tctgcgtcga
    26161 acggccagac gaccagcatg cgtaactgca agagatagaa gcccgctgga tcatcagcgg
    26221 gaaagcctct cttcaccgcg tcagcgccgt aggagataga gcgaaaattg ccctcggtga
    26281 caacggtgtc gtcgtcgacg acgattcggt cgatatccag ctccaaacag aactggccac
    26341 ccttgacgat cgtctcgtca tagaacgcgc ggaccgcttg ccgtcctacc gggctgaaat
    26401 cctcgggagc gccccaggcg atgtagcgcg ggtcggggca gagggtcgac atcaccagat
    26461 cgagatccgg ctttgcctcg cccatcgcat gttggagcac ccgattcaac atgagcagat
    26521 ggcgcggatt ggtcgtctcg gcgatccgct gctcgagcag cctgaacgtc ttggtcgggt
    26581 cgatgatgtc ggtcatcttg tcctcctggc aggcggacag aatttttcca cgcagaccac
    26641 ttcgcgatac cgctattcgt agatgccttc gacgtaccac cgccgctgcc ggtagcacag
    26701 cagcaaggcc tgcgccgggt tcccgtcgag cagcacctgc gccctcgcgg tccggccggc
    26761 cttggcttgc tcggaatccc accaccgctc gtcgaccggc catggcccgg tccaccaggc
    26821 cagccggtcc tcccttcccg gccccgccag cagccatggg tcggcggaaa acagcccgcg
    26881 accggtcacc cgtaccgggt tgccgtcggc gtcgagcagc tcgaccgggt cgtcgagcag
    26941 cacggtcggc gacggctccg gaagctgacc gggccacggc tggctcggat cggccctcgg
    27001 caccggctcg tcgcccagcg gtgtgaaggt gatgcgctcg gcaggtccgc ggccgccgct
    27061 gagcaccggc acctgcaccg cctcggggcc gagcagcccc tggacccgca ccaacgcccg
    27121 gcgggcgcgc aaccgatcct cctcaccgat gccaccccac agcggcagtt gcagcgcctc
    27181 ggccgacacc acctccaccg gccgtagccg gatcagcgtc accggtgcag tcggccggtc
    27241 gtccgggtgg cgcttgttca gccagccgtc cagctgccag cgcacccggt cggcggtggc
    27301 gtcctcggtc agcggttcgg cgcaacgcca aacccgttcc agctcttgac cactggtggt
    27361 gacggcatgg atggccaacc gggtgcatcc gacgccagcg gcctcgaggc tgcggtgcag
    27421 ggtgctggcc agcgaccggc cggcgaatgc ggcggcatcg acccgatcga tcggtggatc
    27481 gcaattcatc accgcgtcga gttcggccgg tggttcgcgt cccgacggtc cgcgggttgg
    27541 ctcgccacgg gcaaaccggt gcgcggcgat ggcgtcggcg ccgaaccggg acgccacatc
    27601 gcttcgggac aatgcggcga actggccgat attgcggatt cccatgcgcc acaacagatc
    27661 tgccagatcc tctcggccgg gtgcggccag gctcggctcg gtggccagtt gccggatcga
    27721 cagtgcggac aggaacagtg cgtccttgcc cgcctcgacg atccgtcccg cgcgtgcggc
    27781 gaagacggcg gtggacaact ggtcggcgat gccgacctgg cactcggccc cggccgcagc
    27841 cactgcgtcg accagtcgct cagcggcggc ttgttcggac ccgaagtagc gggccgcacc
    27901 gcgcacggac aacaccagca ggccgggccg cagcacctcg gcccgcggca ccaggtcgtc
    27961 gaccgcggcc gtcacgactt cgaaatggcg ggcatcgcgc gcgggatcgg cggtgacgac
    28021 gtacagttcc ggacaccgtg cctgagattc cctgcggcgc aggcctcgcc gcacgccggc
    28081 cgcacgggcc gacgccgagc aggcgatcac ccggttggcc agtgtgaccg caaccggcgt
    28141 cgtgggcgca aggcccgccg ccgccaacgc ggcgaccgca ggccagtcca tgcaccagat
    28201 ggccaacact cgcgaggtcg aaccggaaga cactcatccg cctaccgcac gaacggtgcc
    28261 aaaggatcgt cccctggccc gcatggccag ccgcaccttg ctgatgcggc cgatacccgg
    28321 gcgaccgggg ccagtgatct cgtagccaca gaccctggcc tccagccggt tggacgcccc
    28381 ttgccagtcg ccaccggtga caagcagggt gcagcccttc tgccgcgccc gggccaccac
    28441 cgcccttgcc cggcctgctg gcactgtccg gccacccagg cccagcacca ccagatccat
    28501 gccatccatc agcacggcgg ccacctcgac cggatcggcg ccgggttcgg ggatgaccgc
    28561 gatcctgctc agatccgcgc ccatttcgac ggccgcgagc aggccgacgt cgggctggcc
    28621 gacgatcgcg gcgtgcccac cctccgccgt caccgccgcg accatgctca gcgccagcga
    28681 ccgggcgccc gacagcaccg ccaccgtccc acgcggcaac gaggttggaa gcagttcggc
    28741 cagcgcttcg ggcaacggca gcaaagattc tgagtccgga agcgggtcga tcgacggtgc
    28801 tgggccacga cgcccgtttt cgaccttgct agataccgcc gcgatcttgc ggcgcaggtg
    28861 ttctacctga tcagcacggt cgagcaggcg ctgttccagg ctcatggcta ctgtcacagc
    28921 ggcacctccc gcatcatcag aaccacgctg ttttcgaatg tatgttcgat atcccgagta
    28981 aacacccacc caccgacagc gtcaagcggg ctaacggttc atcgagcgaa gaggactact
    29041 cccactcgat ggtgccgggt ggcttgctgg tgacatccag cacgacccgg ttgacctcgg
    29101 cgacttcgtt ggtgatgcga gtggagatac gttccaggac ctcgtagggc acgcgcgtcc
    29161 agtcggcggt catcgcgtcc tcactggata ccggacgcaa cacgatcggg tggccgtagg
    29221 tccgcccgtc gccttgcacg ccgaccgacc ggatgtcggc cagcagcacc accgggcact
    29281 gccagatctg gttgtccagg cccgcggcgg tcagttcctc gcgggcgatc gcatcggcgc
    29341 ggcgcagcgt gtccagccgg gcggcggtga cctcgccgac gatccggatg cccaggccag
    29401 ggcccgggaa tggttggcgt gcaacaattt cctcgggcag gcccagttct cggccgaccg
    29461 cacggacctc gtccttgaac agcagccgca gcggctcaac gagtttgaac ttcaggtcgt
    29521 ccggcaggcc gccgacattg tggtggctct tgatgttggc tgttcctgtc ccgccgccgg
    29581 actccaccac atccggatag agcgtgccct gcaccaggaa ttcgacctca gaaccatccc
    29641 ccgcaagcgg gaggtgcccc cagtcccccg cgacgatgtc tcgtactgcg ccctcgaacg
    29701 cgcgaatgaa ctggcggccg atgatcttgc gcttgccctc cgggttggtc accccggcca
    29761 gcgcctcgag gaaccggtcg gcgacgtcga cagtgaccag attggcgccg gtagccgcga
    29821 cgaagtcgcg ctgcacctgc gcccgttccc cggcgcgcaa cagcccgtgg tcgacgaaca
    29881 cgcaggtcaa ccggtcgccg atggcgcgct gcaccagcgc cgcggccacc gccgagtcca
    29941 cgccacccga gagtccgcag atggcgcggc cgttgccgat ctggttgcgc acctgctcga
    30001 ccagcgagtc ggcgatgttc gcgggcgtcc acgtcgcccc aatgcccgcg aactcgtgca
    30061 ggaagcggct cagcacctgc tggccgtgcg gcgagtgcat cacctcgggg tgatattgca
    30121 cacccgcgag cctgcgcgca cgatcctcga atgccgcgac cggcgctccg gcgctggtgg
    30181 ccaccacgtc gaaaccgtcg ggagcggccg tgacggcgtc gccgtgactc atccacaccg
    30241 gctgggtccc cggaagatcc gaatgtagct gtcccccaga aactttcagg tcggtgcggc
    30301 cgtactcgct ggttccggtc ttggcgaccg tcccgccgag cgcctgcgcc atggcctgaa
    30361 acccgtagca gatgccgaac accggcaccc ccagatcgaa caccgccgga ttcaactgcg
    30421 gcgcgccctc ttcgtacacg ctggacggtc cgcccgacag cacaatggcc tgcggatcct
    30481 tgtccttgat gtcctcgacg ctggcagtgt gcgggatgac ctcggaatac accctggcct
    30541 cgcggacgcg gcgggcgatc agctgcgcat actgtgcgcc gaagtcgacc accaaaacgg
    30601 ggcggggcga tgctgattcc accggttcag tctagaggcg gcgatcgacg ccaacgcgtc
    30661 ccagcgcaca tcggtcacac cgtggcgccg tcgacgtaac tggtgatcat ctcgacgagt
    30721 gcgcgacgtt cgcgacgtgt gtccaggggt tcggggatca ccagcgcgtt caccaccaga
    30781 ccgcgcagcg tggcccagat caggttgccg atcgctgcgg cgtgccgggc atccagccct
    30841 tcgccgatct tcttgccaag cttggccatg gcggtgccga gcgcggccag gtgctcgctg
    30901 gcaccagggt cgcgcatggc gcgggtggcg atcaggattt ccagtgccgc catcgaagtg
    30961 ggactggaga acgcctccca cgccgcatcg acgatgaatt ccgttcggtc gcggacgttt
    31021 tcggctgcag cgggtagttc gcgcagtgcc tcgagcagct cggcaaagcc cttgtcgacg
    31081 acggccatca gcagcccgtc gcggtccccg aagtggtact gcaccacccc ccatgtcacc
    31141 ccggcccgct cggtgatgtg tttggcgctg gcggcggcga acccctcatc gagtatgcag
    31201 cggatcgtct cgccgatcac caacgcgcgg gtgcgatcgg cgcgcagttg gctgccgttc
    31261 gcgggccggc ggggcgcaac taggggtgcc attcgaatca ccacatcaca ccgcggatgt
    31321 gcgcggcggg aagatcgcgc gcggccagta gaccgctggg cgcggtgcac accggttcga
    31381 tcatgttcac ggcgcgggcc gcggtggcga tcacgccgcc ctggttgtga tcgacgccgc
    31441 ccaggccgac gtgcgcgttg atctcgacac ccggatcgcc cgcgacggtg acgcggtgga
    31501 cgccggggcg tccgtccggc ggataaggcc agtcgggagc cgcgacgtcg gtgagccggt
    31561 tgacgtgttc catggtgatg acgggctcgc cggcacgcac accctcgact gcgaaccgaa
    31621 cagcggccac ccctcccgct ggcacggtca tcatcgcgca ctcgatggat tgtggcgtca
    31681 cccaggtttc gtggcgttcg cgaacatcgt cgagctcgac accgagaccg tcagcgaggc
    31741 ttc
//
LOCUS       DASRJA010000699        13802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_94938,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000699 DASRJA010000000
VERSION     DASRJA010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13802
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94938"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFC01_31590"
     CDS             <1..441
                     /locus_tag="VFC01_31590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HZN84198.1"
                     /translation="NLQTDIEDPGERLQAIARSDSRAKEHSRAIGPTLVLDWTQLITR
                     AMFGFVLGVAGHTSLTHTAIHNVIISNVAGPRNKLYFLGAEIKALYPLGPIFHGSGLN
                     ITVMSLSGKLNVGIISCQQLVNDLWGLADRFEPELNELLGCRNP"
     gene            complement(442..942)
                     /locus_tag="VFC01_31595"
     CDS             complement(442..942)
                     /locus_tag="VFC01_31595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN84199.1"
                     /translation="MRYRDQPTIEVTQRVRCDVATAWKYVTDITLPARCSAELQSVEW
                     LDDADGVAVGARFRGRSQHDAFGNWETVCEVVEVEDQRRWVWNVIGPGGVGATWGFEV
                     EPASDGVLIRQWGRMGPGPSGLTAGIAAQPEKEARIVARRLSEWQQNMQANLDWIRSQ
                     VEGRLT"
     gene            1019..2032
                     /locus_tag="VFC01_31600"
     CDS             1019..2032
                     /locus_tag="VFC01_31600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN84200.1"
                     /translation="MIEPRLPGAAEFAVAAERIGVDSLWVPEVWGYDALTGLAYLAAK
                     TSTIKLGTFVVQLGSRSPAMLATSALSLQELSDGRFLLGVGTSGPGVMEGWHGVRFRK
                     PVETTRETIEIVRIVSRGDRLEHPGEIYPLPLPDSSGNALRPLVNPRHVPVYVAAMGP
                     RNLRLAGEMADGWLGNAFIPEAAEVFFGPLREGAERAGRTLAELDLVAPVAVEIHDDE
                     ASADAAARRHADGYAFTIGAMGAGGRNFYNDAFARLGYGDEVSRVAALWQDGRRDEAR
                     QAVPIDLGRLTNLVGTEGGIAERVRKYRAAGITTLLAKLEGDYPYQLTTLERLVEIVA
                     DSG"
     gene            complement(2069..2539)
                     /locus_tag="VFC01_31605"
     CDS             complement(2069..2539)
                     /locus_tag="VFC01_31605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84201.1"
                     /translation="MDLPSWGLGLSLIVLTVAIHTTAVVLMAFGLEPRIRVRLDKHQP
                     HVLRAIPTVIAHIGAVALILAALHGLEGVLWALAYWWLGALDSLTDASTYSLASMTTF
                     ELPGLTVPSRFNMLSVLEAVNGALLFGISTAFLFAVMQVHWQLLSHRHERWNDH"
     gene            2775..3566
                     /locus_tag="VFC01_31610"
     CDS             2775..3566
                     /locus_tag="VFC01_31610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HZN84202.1"
                     /translation="MGEFGIAGLWLFLAIALVTSVLVAAGSVWLADRTLTKPVSEGHN
                     GSLSPFLTCVGLVYGALLGFTIVVAWEQFSSAEANVSNEASTLTTMYRQTVGMPVPEQ
                     TQMRELLRNYAKAVEGPEWQNAIRAGTGTEGARDALNDMYRVLGGEQSSTASNPISQK
                     FLDQLTTLASERNQRILDSKPRIPGLLWTGLIFGGVVLLGLGGFMRLGSARGHLVLLG
                     AVAVLLGLLLFIVYWLDHPFGNQLGVTSAPFEQSLSVFDSIDRGT"
     gene            complement(3567..3794)
                     /locus_tag="VFC01_31615"
     CDS             complement(3567..3794)
                     /locus_tag="VFC01_31615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84203.1"
                     /translation="MRSGRLLVAAGAVGVVAITGAPSALAEPVATCTSDVVDGVEVDY
                     CVGNPNANTVTGVPGVNVDFEFGLGIAFGFG"
     gene            complement(3801..4202)
                     /locus_tag="VFC01_31620"
     CDS             complement(3801..4202)
                     /locus_tag="VFC01_31620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN84204.1"
                     /translation="MRSTEIVNAFWDDVWNAHDPDAVDKFVADDVVIEAGGQEIAGKD
                     NVKNWVKEFLDHVNDLHVDTIETFQNEDGSRVTSRWVLTGANNGVLGTEPNGKPVALT
                     GTAVWTVDDGKLQRGWVEQASFELYHYLLAK"
     gene            5106..6464
                     /locus_tag="VFC01_31625"
     CDS             5106..6464
                     /locus_tag="VFC01_31625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZN84205.1"
                     /translation="MSLIHGWLPVTVQVITAVVLVVAIGWRSRRWRMISVPVGLFVGV
                     ALTGAVSWFIADQGLADHQSPLGLWVWITLTGLAAVVVVLGWRDVHWWRRGVSVLAVP
                     LCVLCTALTVNMWVGYFPTVGSAWDGMTGAPLPGQTDRATVAALQQQHRAPDWKTIVS
                     VRIPDHASGFKHRDELVLLPPAWYASSPPPPLPVVMMIGGEFGNPAAWLYAGDAQSTI
                     VDFASAHGGNAPVLVFPDSSGTFSNDTECVNGVRGNAADHLTKDVVPYIISNFGVSRD
                     PANWGIVGWSAGGTCAVTLSVMHPELFRAFVDIDGQLGPTAGTKQQTIARLFGGDADA
                     WAAFDPRTVIAKHGAYSGMSGWFAVSVDTPTVYRAPTDAGPLPADDTDPKPEDHAAIA
                     NSLCQSASSHGIECAVVSNPGKHDFPAAASAFAAALPWLAGKLGTPGVPPIAMPGAPR
                     PR"
     gene            complement(6539..7426)
                     /gene="aqpZ"
                     /locus_tag="VFC01_31630"
     CDS             complement(6539..7426)
                     /gene="aqpZ"
                     /locus_tag="VFC01_31630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HZN84206.1"
                     /translation="MTAPTMFHRLAAEMLGTFWLVLGGCGSAVFATSVFLPEGVSVGI
                     GLLGVAIAFGLTVLTGVYAFGTISGGHFNPAVTLGAALAKRVEWKTLPAYWTVQVIGG
                     LLAGLVIYLIANGKQGFTPTGNMAANGFGAHSPGGYSLTAVIVTEIVLTLVFLLVILG
                     STDDRAPKGFAGLAIGLTLTAIHLVSLPISNTSVNPARSMGVAFFNGNGAPAQLWVFW
                     LAPLIGAAIAGIAYPYLFGKREQLAGRPVRDDRLETDDSGQHRPAEAWSRGGDPQPRR
                     RPPPAPGSWPPQLPRREPR"
     gene            8352..9956
                     /locus_tag="VFC01_31635"
     CDS             8352..9956
                     /locus_tag="VFC01_31635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN84207.1"
                     /translation="MTVGEAQVWGLRVKRTAVAFGAVLLFVALVVFSGPFGGDHHSAP
                     QPSVPSASGDGSSAATQQEQPAPGAHPEGSSGGALPPAQGVQAFSGGTEPRAQPMPPY
                     QSENHPLSWPQFLLNGVLVILSLGLFTVASTTLWWMLHAWRSPDALAATGFRRRAAGA
                     PRSFSLLLPARHEQDVLGDTIDALARLDHPKYEVIVIIGHDDPETECVAREAAARYPR
                     IVRVVMDYHLPKNKPKALNTALRKCRGDIVGVFDAEDEVHPGLLRLVEARFEESGADV
                     VQAGVQLMNVQTSWWSLRNCLEYYFWFRSRLHFHAEKRFIPLGGNTVFTRTELLREAG
                     GWDPDCLAEDCEIGVRLSTRGAVVAVAYDPQAVTREETPGSIRALVKQRTRWDQGFLQ
                     VLRKGEWRKLPSPRQRWLARYTLAMPLLQAATGVLMPLSLASMFLLKVPMPTALVTFL
                     PLAPTLVTMAVESAALGEFGREFGIRIRFRDQARLLLGTVPYQLLLAAAAVRSVWREG
                     RGQGGWEKTTHTNAHRTTTPKRPVPA"
     gene            9953..13105
                     /locus_tag="VFC01_31640"
     CDS             9953..13105
                     /locus_tag="VFC01_31640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZN84208.1"
                     /translation="MSRSSRLNPSIPILGILLILTATLRLINLTGSPQRLDDEGTYMA
                     QAYAVVKWGELAHYTYWYDHPPAGWLQLALWTAISGPDFGGNAVAAGRYLMVIVAVIT
                     AALLWALARRIEMSRWASAAAVAFFALCPLAISLSRMVYLDNLAVAWVLGALVLLCSP
                     QYRLSAMFGAATCFGIAVLTKETMLLLLPMFAWLVWTRTAPATRRYALAVFVALFGVV
                     VSTYVLMAVLRGELVPGPGHVSLWDGIRFQLWERTTGGELDDPGSLKRNVVNKWLQFD
                     PGLPFLAVPISLAALFVKRLRPFAVGLVILFATILRPGYLPGPFILSALPLTALLAAG
                     TGEVALRYLFRAVEQRSVGLRRFRVPALAAGALIVSIAVSLWLPTYHRLMESDDDAPM
                     RQAQQWIQRNVPKTDRLIVDDAFWLDLIRDGRDRRNVVWASKVDTDKQVQGWAPHGWT
                     DYEWVVSTVSLRAGMPPNGVFADAVAHSQPAATFGSGSERVDVLRVDNGAPTSKPPSP
                     AAPAFGGQLAAHLAGATKPDVLAVLQSRTVDQRVLATLAVIAATQPVQVEDISTIEGE
                     DNTGTARRDITLRGPRDRLQSVAAFFERQVGAFAVQSVHLTATGLRVRFPLRTKDIGL
                     GTVPAPLQGGPAALRVADLRRSRPAEQLNLVRIDGTAAGSIDTSDDANPSSYRSMPAG
                     TYVVMTNRPGGGDPVIRQVLTLHPGVTYTLALFSAAESSQLAVQLAPDGPPTGPTPDS
                     AVRMLHAASAAGSVYLALVAPGAVEPIVLANQAGYGLITGYAALPAGQYEAVVSSNGR
                     EWRYPVELVGGEPVTLLLTDGPDGPVLRMLRDVPEAPAALNPPTLTMPASGSAVEKTT
                     GKTHATERSDGRRIAVVLCAAAIMGAAVLMARARPTRRPGQQNDAALHEAVRPPRREV
                     PRRARPVDDTARLPRGLVKPSARPRRPRQHNDVALHQAVRARGPGGEVARRARPVNDT
                     ARLDLDVDHTVRLPIGAVKLSAPLRWPRPKNAAALHPAVRRRGHARPVDDTARLALDV
                     DNTPRLPTVADKPSVPREPR"
     gene            13102..>13802
                     /locus_tag="VFC01_31645"
     CDS             13102..>13802
                     /locus_tag="VFC01_31645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HZN84209.1"
                     /translation="MKRQLRTASVIALVLALMAGAGWWIANAIDPRLDAADIAGLRED
                     TARIAGQKFLDEYVEPDGRVVRRDEGGDVVSEGQAYGMLIAVAVGDETRFRSIWKWTK
                     THLRRADGLLAWRWADNEVTGVNSAADADLDAARSLVLAGRRFDAPELTMDGRRLGVA
                     ILETETVAVGTAAAPSSDVNPPGTWVAGSGRTLVGGNWAMTAPYIVNPGYFSPRAEWE
                     LFEASADRRWTDITRT"
BASE COUNT         2331 a         4347 c         4665 g         2459 t
ORIGIN      
        1 aacctccaga ccgacatcga ggatccgggc gaacggctgc aggccatcgc cagatcggat
       61 tcacgtgcaa aggaacacag ccgggccatc ggtccgacgc ttgtgctcga ctggacacaa
      121 ctcatcaccc gggcgatgtt cggcttcgta ttgggcgtgg ctggtcacac ctcgctgacg
      181 cataccgcga tccacaacgt gatcatctcg aatgtggcgg ggccgcgaaa caagctgtat
      241 ttcttgggcg ccgaaatcaa agcgctgtat ccgctcggcc cgatctttca tggctcggga
      301 ctgaacatca cggtgatgtc gctcagcggc aagctcaacg tcggcatcat ctcgtgccaa
      361 caactggtga atgatctgtg gggcctggcc gaccgtttcg agcccgaact aaacgaactg
      421 cttggctgtc gaaacccata gtcaggtcag gcggccctcg acctgcgacc ggatccagtc
      481 gaggttggcc tgcatgttct gctgccattc ggacagccgc cgcgcgacga tcctggcctc
      541 tttctccggc tgcgcggcga tcccggccgt cagccccgac ggtcccggcc ccatccggcc
      601 ccactgccgg atcagcacgc cgtcgctggc cggctccacc tcgaaccccc aggtcgcgcc
      661 cacaccgccg ggacctatga cgttccatac ccagcggcgc tgatcctcga cctcgacgac
      721 ctcacacacc gtctcccagt tgccgaacgc gtcatgctgg ctgcggcctc ggaagcgggc
      781 tccgaccgcg acgccgtcgg cgtcgtcgag ccattcgacc gattgcagct ccgccgagca
      841 ccgcgcgggc agagtgatgt cggtgacgta tttccacgcg gtggcgacat cgcagcggac
      901 acgctgggtc acttctatcg tgggctgatc gcggtagcgc accgttgcag catgtcaagg
      961 atgcgcgacg gggcgtccgg aacggcagga aagcggggca acggtgaagg tcggcaccat
     1021 gatcgagccc cggttgccgg gcgcagcgga gttcgccgta gccgccgagc ggatcggtgt
     1081 ggattcgttg tgggtgccgg aggtatgggg ctacgacgca ttgaccgggc tggcgtacct
     1141 ggcggcgaag acgtcgacga tcaagctggg aacctttgtg gtgcagctgg gttcgcgcag
     1201 tcccgccatg ctggcgacgt ccgcgctgag cctgcaggaa ctgtctgacg gccgattcct
     1261 gttgggtgtc ggcacgtcgg ggcccggggt gatggagggc tggcacggcg tccggttccg
     1321 caagccggtg gagacgaccc gagaaaccat tgagatcgta aggatcgtca gccgcggaga
     1381 ccggctcgag catcccggcg agatctatcc gcttccgctg ccggacagca gtggcaatgc
     1441 gctgcggccg ctggtgaatc cccggcatgt gccggtctac gtcgcggcga tggggccgcg
     1501 aaacctgcgg ctcgccggtg agatggccga cggctggctc ggcaatgcgt tcattcccga
     1561 ggccgccgag gtgttcttcg gtccgttgcg cgaaggcgcc gagcgcgctg gacggacgtt
     1621 ggcggaactg gatctcgtgg caccggtcgc tgttgagatt cacgacgacg aagcgtcagc
     1681 cgacgctgcc gcgcgcaggc acgcggacgg atacgcgttc accatcggcg cgatgggtgc
     1741 gggcggtcgc aacttctaca acgacgcgtt cgcccggctg gggtacggag acgaagtgag
     1801 tcgggtcgcc gctctgtggc aggacgggag acgggacgaa gcccggcagg cagtgccgat
     1861 cgacctcggc aggctgacga atcttgttgg tactgaaggc ggtatcgctg aacgggtgcg
     1921 caaataccgg gcggcgggaa tcacgacgct cttggccaaa ttagagggcg actacccgta
     1981 tcagttgacg acgctcgagc gactggttga gatcgttgcc gattcaggat gagccgtctt
     2041 caggcgctgc ggaccagtgc acgccgcatc aatggtcatt ccaccgttcg tgtcggtggg
     2101 agagcagctg ccagtggacc tgcatcacgg cgaaaaggaa cgccgtgctg atgccgaaca
     2161 gcagtgcgcc gttcaccgct tctaacacgc tcaacatgtt gaaccgggac ggcacagtca
     2221 accccggcaa ttcgaacgtc gtcatgctgg ccagggaata ggtcgaagcg tccgtaagcg
     2281 agtcgagcgc gccgagccac caatacgcca atgcccacaa cacgccctcc agcccgtgca
     2341 gagcggcgag tatcagcgcg acggctccga tgtgggcgat aacggtagga atcgctcgca
     2401 gcacatgagg ctggtgtttg tcgagccgga cccgaatcct cggttcgagt ccgaaagcca
     2461 tcagcactac cgcggtggtg tggatcgcga cggtcagcac gatgagcgaa aggcccaggc
     2521 cccaactcgg aagatccact cgttaccctt accaccggcc actcctgcag acggccacaa
     2581 cggccgaagt ggattgcgcg tacaggtgct cggtgcgagg agcatgctcg gtgggcccag
     2641 gagcaagacc ttgccgggct cacggccgat tcagcagccg aagcgactat gtgcgggtgc
     2701 tgaagggtat ccagaatgat tgttgtcagg ccgcccggcg cgggtggtcg gcgccttacg
     2761 ggtggaggta ggccatgggc gagttcggga tagcgggtct ttggctgttc ctggccatag
     2821 cccttgtcac gtcggtgttg gtcgcggccg gcagtgtgtg gctggcggat cgcaccctca
     2881 cgaaaccggt gagcgaaggg cacaacgggt ccctgtcgcc gtttttgacc tgtgtcggac
     2941 tcgtttacgg ggctctgctc gggttcacga tcgtggtcgc gtgggagcag ttctcctccg
     3001 cagaggccaa cgtgtccaac gaggcgtcca ctctgacaac gatgtaccgg cagaccgtcg
     3061 gcatgcccgt ccccgagcag acccagatgc gggaactgct ccgcaattac gcgaaggcgg
     3121 tagagggccc tgaatggcag aatgcgatac gcgccggcac tggaaccgaa ggcgcgcgtg
     3181 acgcgctcaa tgacatgtac cgggtgctcg gcggtgagca gtccagcacc gcgtcgaatc
     3241 cgataagtca gaagtttctc gaccagctga ccactttggc gtcggagcgg aatcagcgaa
     3301 tcctggacag caaaccgcga atacccggct tgctgtggac cgggctgata ttcggcggtg
     3361 tcgtactgct gggactcggc ggtttcatgc gcctggggag cgcgcgcggt catctcgtcc
     3421 tgctgggcgc ggtcgccgtc ctgctcggct tgctgctgtt catcgtctac tggctcgacc
     3481 atcccttcgg gaaccagctg ggcgtcactt ccgcaccgtt cgaacaatca ctgagcgttt
     3541 tcgactcaat cgaccgtggg acatgatcag ccaaagccaa aggcgatccc caggccgaac
     3601 tcaaagtcca cgttcacgcc cggcacacca gtgacggtgt tcgcgttggg gttgcccacg
     3661 cagtaatcca cctcgacacc gtcgacaacg tcgctggtgc acgtcgccac gggttcggcc
     3721 aaggcgctgg gtgcgccggt gatggcgacg acgccgactg cgcccgccgc caccaagagt
     3781 cggccggacc gcattagccg ttacttcgcg agcaggtaat ggtagagctc gaacgaggcc
     3841 tgctcaaccc agccgcgctg cagctttccg tcgtccacgg tccagaccgc ggtgccggtg
     3901 agcgcgacgg gtttgccgtt gggttcggtg ccgaggacgc cgttgttggc gcccgtcagc
     3961 acccatcggg acgtgacgcg gctgccgtcc tcgttctgga aggtctcgat ggtgtcgacg
     4021 tgcaggtcat tgacgtggtc gaggaactcc ttgacccagt tcttgacgtt gtctttgcca
     4081 gcgatttcct ggccgcctgc ctcgatgacg acgtcgtcgg ccacgaactt gtcgacggca
     4141 tcggggtcgt gcgcgttcca gacgtcgtcc cagaaggcgt tgacaatctc cgttgatcgc
     4201 atgtctcaac gatccccctc gcaagcactg cccgcaggtc tttcgaccgc atgtggggca
     4261 gcagtttcaa tggatgacga cgctggactg agcggacatc ctgtcgcgtg cccaggggcc
     4321 tcaggtttgt gaaagcaact ggaccttaag gggtttgact ggctgccgac gtgcgtatgg
     4381 ttcaacgacc cgcggcgcta gtcaccgtcg ccgtgcccat ggcgggagcc accgtggcca
     4441 ggccacggcc ccctcggacc cgggttgtag taggggggtg gctgaagaat cggtgggagc
     4501 cacgggatga gcggcggtgg aaccacaaag ggcggcggag cggctagtgc gggtggcggc
     4561 ggcggggcgg gcagaacctt cagccgcgcc gtcgcgatcg gcgcaggtgc cgcttccacc
     4621 catagcggtc gaggtcgcgc ggcggccgat gaattcggtt gtgcgacatt gcttttgtga
     4681 gggtgtgcgg cacaggctct ggggaaagcg cttcacctac ggagtgcttt ccagtttgcg
     4741 gatcttgaga cgtcgtaatc ccaagtttcc cctgccagat aggcacctcc gtgcatttac
     4801 ccgcacacaa taacgatcac ccgtgaacga tgcgggctaa cgagcgtgtt gtagtgacgg
     4861 ttgatcgcgt cacgggacag gcgcgtcgga cggggcccct ttagtcacgc gaatagcgtc
     4921 tactaacagc tcaacgacaa acctcgtcgg cgtaccgtgg actctgccaa cgtggcgcca
     4981 tcgagccggt ggtgtcgcag aagtggcaaa ccggacaggg agtccgcctg ctgagtcaca
     5041 gcggccggtg ccgatcgccg ggccgttgta gcccaggcgt ttcgatcatg gaggactgac
     5101 ccggaatgtc tttaatacat ggctggctgc cggtgacggt gcaggtgatc accgcggtgg
     5161 tgctggtggt ggcgatcggc tggcgatctc gccggtggcg catgatctcg gttcccgtgg
     5221 ggcttttcgt cggcgttgct ctgaccggag cggtgagctg gttcatcgcg gatcaagggt
     5281 tggccgacca tcaatcgccg ctgggtctgt gggtgtggat cacgttgacc gggctggccg
     5341 cggtggtggt cgtgctggga tggcgcgacg tccactggtg gcgccgcggt gtctccgtcc
     5401 tggcggttcc cctatgcgtg ctgtgcacgg cgctgactgt gaatatgtgg gtgggttact
     5461 tccccacggt cggttcggca tgggacggaa tgaccggtgc gccgctgccc gggcagaccg
     5521 accgtgccac ggtcgccgcg ctgcagcaac agcacagggc gccggattgg aaaaccatcg
     5581 tgtccgtgag aatccccgat catgcatcgg gattcaaaca ccgtgacgag ctcgtcctgc
     5641 tgccgccggc gtggtatgcc agctcgccac cgccaccgtt gccggtggtg atgatgatcg
     5701 ggggtgagtt tgggaatccg gccgcatggc tctacgccgg cgatgcccaa agcacgatcg
     5761 tcgacttcgc gtcggcgcac ggcggcaatg cgccggtgct ggtgttcccc gattccagcg
     5821 ggaccttcag caatgacacc gagtgcgtga acggtgtacg cggcaacgcc gcggatcacc
     5881 tcaccaaaga cgttgtgccg tacatcattt ccaatttcgg ggttagccgg gacccggcca
     5941 actggggtat cgtcggctgg tcggcggggg gtacctgcgc ggttacactg agcgtgatgc
     6001 atccggagct gttccgcgcg tttgttgaca tcgacggcca gctgggtccc actgcgggca
     6061 ccaagcagca gactattgcc cggctgttcg gcggcgacgc cgatgcctgg gcggctttcg
     6121 atcctcggac cgtgattgcc aaacatggtg catacagcgg aatgtcgggc tggttcgcgg
     6181 tctcggttga cactcccacg gtgtaccgcg cgccgacgga cgccggtccg cttcccgcag
     6241 acgacacgga tccgaaaccc gaggaccatg ccgccatcgc caatagtctg tgccaatcgg
     6301 ccagcagtca cggcatcgag tgcgcggtgg tgtccaaccc tggtaagcac gacttcccag
     6361 cagcggcgtc ggcgttcgct gcggcgttgc cctggctcgc cggaaagctc ggaacacccg
     6421 gggtgccccc gatcgcaatg ccgggcgccc cccgaccccg gtgatgtgac gtctcggtca
     6481 agtcgcacaa caccattgag cctccccgcg gtttgacatt tgtatcgcgc agctgtgatt
     6541 accgcggctc gcgacgcggc agttgcggtg gccatgatcc tggagccggg ggtggtcgtc
     6601 gacgaggttg aggatctcca cctcgggacc acgcctccgc cggtcggtgc tggcctgagt
     6661 cgtcggtttc caacctgtca tcgcggaccg ggcgcccggc gagttgttca cgcttgccga
     6721 acagatacgg gtacgcaatt ccggcgatcg cagcgccgat gagcggtgcg agccagaaca
     6781 cccagagctg cgcgggggcg ccgttaccgt tgaagaacgc gacgcccatc gaccgcgccg
     6841 ggttgaccga cgtgttggag atggggaggg agaccaggtg gatcgccgtg agggttaggc
     6901 cgatcgccag cccggcaaat cccttgggcg cccggtcatc ggtggatccc aggatgacaa
     6961 gcaggaacac aagagtcagg acgatctcgg tgacgatcac tgccgtcagt gagtagccgc
     7021 caggagagtg cgcgccgaat ccgttggccg ccatgttgcc ggtcggggtg aagccttgct
     7081 tgccattggc gatgaggtag atcaccaggc cggcgagtaa accgccgatc acctgaacag
     7141 tccagtaggc cggcagagtc ttccactcca cccgtttcgc aagcgccgcg cccagcgtga
     7201 ccgcgggatt gaaatgcccg ccggagatcg tgccgaacgc gtagacaccc gtgagtacgg
     7261 tcagaccgaa ggcgatagcc actccgagta gacctatccc gaccgaaaca ccctcgggga
     7321 ggaacaccga tgtggcgaac accgcgctgc cgcagccgcc caagaccaac cagaaggtgc
     7381 cgagcatttc cgctgccagt cgatgaaaca tggttggagc agtcatgtgc cgccgccttc
     7441 gccgttgatt tggtgtgcct tggcttgagc gtggcacaaa agctcgtcac aatcccaggc
     7501 cccttccagg gccccggtgg ggcgcctgaa cggagcaaac cctgagcagc accgcacccc
     7561 cggcggacac gcccaggggt gggaattgtg tgatggcggg tggggaacga aatgacggac
     7621 tatccctcaa acctggctat tgcgtgaccg ctgacaataa gatattgacg cgcggggaat
     7681 cggtgcagac gcgctggcgc ggctgcgtag tggctcactg cgcacgaccg gttgacaaga
     7741 ctgcgcgact agatctggac acggtggccg ttaaacctgc ggctccgccg caggggccga
     7801 gaccgcagaa caatgcttca ctgcatgacg cggtgcgggc gcattggcgt gaggtggttc
     7861 gccgcgtacg acccgttgac aacactgcgc gactggatct cgatcttgac aacactgcgc
     7921 gagtagatcc cgagacggcg gccatcaagc ctccggctcc gccgcggcgg ccgagacccc
     7981 agaacgatgc ttcactgcat gacgcggtgc gggcgcattg gcgtgaggtc gtttgccgcg
     8041 tacgaccggt tgacaacact gcgcgactcg atctggacgc tgacaaacct gcgcgactag
     8101 atctggatgc tgtcaacacc gcgggactag atccggacac ggtggccgtt aagccttcgg
     8161 ctccgccgcg gcggccggga cagcagaacg atgctgaacg gcgcgaagcg gtgcgggcgc
     8221 gcgggcccgg cggtgacgtg gttcgccgcg cacgaccggt tgacagcgct gccccgcttc
     8281 ggaggggcgc cgctacgcca tcggttccgc tctggcggcc gggacagccg aacgaaactg
     8341 cgttgcacgc ggtgactgtt ggcgaggcac aagtttgggg gctccgcgtc aagcggactg
     8401 ccgtggcgtt cggtgccgtt ctcctcttcg tcgcgttagt ggtcttcagt gggcccttcg
     8461 ggggcgacca ccattccgcg ccgcagccgt cggtgccctc agccagcggt gacgggtcat
     8521 ctgccgcgac gcagcaagag caaccggcac cgggtgcgca tcccgaaggg tcatccggcg
     8581 gcgcactgcc accggcccaa ggcgtgcaag cgttttcagg cggcactgag ccgcgcgcac
     8641 agccgatgcc gccttaccag agtgagaatc atccgttgtc gtggccgcag ttcctcctta
     8701 acggcgtgct ggtgatcctg tcgctggggt tgttcacggt cgccagtacg accctgtggt
     8761 ggatgctgca cgcgtggcgc agtcctgacg cgttggcagc gacgggtttt cggcgccgag
     8821 cagcaggtgc acctcgatcg ttctcattgc tgttaccggc tcgccacgaa caggatgtcc
     8881 tcggggacac gatcgacgcg ctggctcggc tcgaccaccc caagtacgag gtgatcgtga
     8941 tcatcggtca tgacgacccg gagacagaat gtgtcgcgcg tgaggcggcg gcacgctacc
     9001 cgcgcatcgt cagggtggtc atggactacc acctgcccaa gaacaagccg aaggcgctca
     9061 acaccgcact gcgcaaatgc cgcggtgaca tcgtcggtgt ctttgacgcc gaggacgagg
     9121 tgcatccggg gctgctgcgt ctggtcgaag cgcgtttcga ggaatccggc gcagatgtgg
     9181 ttcaggcggg cgtgcagttg atgaacgtcc agacgagttg gtggtcgctg cgcaactgcc
     9241 tcgagtacta cttctggttc cgctcccgcc tgcatttcca cgcggagaaa cggttcattc
     9301 cgctcggggg caacaccgtg ttcactcgca ccgagctgct gcgggaagcg ggcggctggg
     9361 accccgactg tctggccgag gactgcgaga tcggcgtgcg gctgtccacc cggggtgcgg
     9421 tcgtggccgt cgcctatgac ccgcaagcgg tcacccggga agagaccccc ggttcgatcc
     9481 gtgccctcgt gaagcagcgc acgcgctggg atcaaggttt tctgcaggtg ctccgcaagg
     9541 gggagtggcg caagctgcct agccccagac agcgctggtt ggcccgctac accttggcga
     9601 tgccattgct gcaggcggca acgggcgtgc tgatgccgct gtcgctggcg tcgatgttcc
     9661 tgctgaaggt tccgatgccg acggcgttgg tgacgttcct gccgctggcg ccgacgctcg
     9721 tcaccatggc ggttgagtct gccgcgctcg gtgagttcgg acgggaattc ggcatccgaa
     9781 tacgtttccg tgatcaagcg cgactattgc tgggcaccgt tccataccag ctgctacttg
     9841 cggccgccgc cgtgcggtcg gtgtggcgtg agggccgcgg gcaaggaggg tgggagaaga
     9901 ccactcacac caacgcccac cgcaccacca cacccaagcg gccggttccg gcatgagccg
     9961 ttcatcccgc ctgaatccct cgatcccgat actcggaatc ctgttgatac tcaccgcgac
    10021 actgcggttg atcaacctta cggggtcgcc ccaacgactt gacgacgaag gcacgtatat
    10081 ggcccaggcc tacgccgtgg tcaaatgggg agaactcgcc cactacacct actggtacga
    10141 ccatccgccg gccggctggc tgcagttggc gctgtggacg gcgatttcag gtcccgactt
    10201 cgggggcaac gcggtcgctg ccgggagata cctgatggtc atcgtcgccg tgatcaccgc
    10261 cgctctgctg tgggctctgg cgcgccgaat cgaaatgtcg cggtgggcaa gcgcggccgc
    10321 ggtcgccttc ttcgcgctgt gtccgttggc aatctcgttg agccgcatgg tatatctgga
    10381 caatctcgcc gtcgcgtggg tgctgggcgc cctggtgctt ctgtgctcac cccagtaccg
    10441 gctgtcggcg atgttcggcg ccgcaacatg tttcggcata gcggtgttga ccaaagagac
    10501 catgctgctg ctcctgccga tgttcgcctg gctggtctgg accaggaccg caccggcgac
    10561 ccgccgctac gcactcgccg tgttcgtcgc tctgttcggc gtggtggtca gcacgtacgt
    10621 cttgatggcc gtcctgcgtg gtgagctggt ccccgggccc ggacacgtca gtctgtggga
    10681 cggcatcagg ttccagttgt gggagcgcac gaccggcggc gagctcgatg atcccggttc
    10741 gctcaaacgt aatgttgtta acaaatggct ccaattcgac ccgggcctgc cgtttctggc
    10801 cgtcccgatc tctctggccg cactgttcgt caagcggctg aggccgttcg cggtcgggtt
    10861 ggtgatactc ttcgccacga ttctccgtcc cggatacctg ccggggccgt tcatcctgtc
    10921 cgcgctgccg ttgacagcgc ttctggccgc gggcaccggc gaggtggcgc tgcgctatct
    10981 gttccgcgcc gttgagcagc gatcggttgg cctgcggcgc ttccgtgttc cggcgctcgc
    11041 cgccggggcg ctgatcgtgt ccatcgccgt gtcgctctgg ctgcccacct atcaccgctt
    11101 gatggagtcc gacgatgacg cgccgatgcg gcaggcacag cagtggatcc agcggaacgt
    11161 gccaaagacc gaccgtctga tcgtcgacga tgcgttttgg ctggacctga tccgggacgg
    11221 ccgcgaccgg cgcaatgtcg tgtgggcctc caaggtggac accgacaagc aggtgcaggg
    11281 ctgggcgccc cacggctgga ccgactacga gtgggtcgtg tcgacggtgt ccttgcgtgc
    11341 cggcatgccg ccgaacggtg tgttcgctga cgcggtcgcg cactcacaac cagcggcgac
    11401 gttcggctcc ggttcggagc gagtcgacgt gctccgtgtc gacaatggcg cacccacctc
    11461 gaaacccccg tcgcccgcgg cgccggcgtt cggagggcaa ctcgcggcac acctcgccgg
    11521 tgccaccaag ccggatgtac tcgcggtcct gcagtctcgg accgtggatc aacgcgtgct
    11581 tgcgacgctg gcagtgatcg ctgcgacgca acccgttcag gtcgaggaca tttcgactat
    11641 cgaaggagag gacaacaccg gaacggcccg acgcgatatc acgctgagag gcccgcgcga
    11701 ccggctacaa agtgtggcgg cattcttcga acgccaagtg ggcgcgttcg cagtgcagtc
    11761 cgtgcatcta accgccacgg gactgagagt tcgatttccg ttgcgcacca aggatatcgg
    11821 tctgggcaca gttccggcgc cgctgcaggg cggtccggcc gcgctgcggg tggcggacct
    11881 gcgccgcagc cgaccggccg agcaactcaa cctcgtgcgc atcgacggca ccgcggccgg
    11941 ctcgattgac acgagcgacg acgcgaatcc gtcgagctat cggtcgatgc ccgcggggac
    12001 gtacgtggtg atgacgaacc gtcccggcgg aggtgacccg gttatacgtc aggtgctcac
    12061 gctccatcca ggggtgacct acacgctcgc actgttctct gcggccgaat ccagccagct
    12121 cgcagtgcaa ctcgcaccgg acggcccgcc caccggcccc acgcccgact ccgccgtacg
    12181 catgctgcac gcggccagtg cagcgggatc cgtctacctg gcgctggtag cacccggcgc
    12241 ggtcgagccg atagtgctgg cgaaccaagc tgggtacgga ctgatcaccg ggtatgccgc
    12301 gctgcccgcg gggcagtacg aagcggtggt gagttcaaac ggccgggaat ggcggtaccc
    12361 cgttgaactc gtcggtggcg agccggtgac tctgctgctc acggacgggc ccgacggtcc
    12421 cgtgctgcgg atgctgcggg atgttccgga ggctcccgct gcgctgaacc ccccgacgtt
    12481 gacgatgccc gcgagcggaa gtgcggtgga gaaaaccaca gggaaaacgc atgcgactga
    12541 acgctcggac ggaaggcgga tcgcggttgt actgtgcgcc gccgccatca tgggcgcagc
    12601 ggtcctgatg gcgagggcgc gaccgacgcg gcggccggga cagcagaacg atgccgcact
    12661 gcacgaggct gtgcggccgc cccggcgtga ggtgccacgt cgcgcacgac cggtcgacga
    12721 cactgcgcga ctacctaggg gactcgttaa gccttcggct cggccgcgcc ggccgagaca
    12781 gcacaacgat gttgcactgc accaagctgt gcgggcgcgc gggcccggcg gtgaggtggc
    12841 tcgtcgcgca cgaccggtta acgacactgc gcgactagat ctcgatgttg accacactgt
    12901 gcgactacct attggcgccg ttaagctttc ggctccgctg cggtggccga gaccgaaaaa
    12961 cgctgctgcg ctgcaccctg ctgtgcggcg gcgcgggcac gcccgaccgg ttgacgacac
    13021 tgcgcgacta gctctcgatg tcgacaacac tccgcgacta cctacggtgg ccgataagcc
    13081 ttcggttccg cgggagcccc gatgaagagg caactgcgca ccgcgtcggt gattgcgctc
    13141 gtgctggcgt tgatggccgg cgccggctgg tggatcgcga acgccatcga tccgcggcta
    13201 gatgcggccg acatcgccgg cctgcgggag gacacggcgc gtatcgccgg ccagaagttc
    13261 ctggacgagt acgtcgaacc cgacggacgg gtcgtgcgac gtgacgaggg cggtgacgtc
    13321 gtaagcgagg gccaggcgta cgggatgctc atcgctgtgg cggtcggtga cgagactcgg
    13381 ttccggtcga tatggaagtg gacgaagact cacctgcgcc gcgccgacgg cctgctggcc
    13441 tggcggtggg ccgataacga ggtcaccggt gtcaacagcg ccgcggatgc cgatctcgat
    13501 gcggcgcggt cgttggtgtt ggccggccga cgcttcgatg cgccggagct caccatggac
    13561 ggcaggcggc tgggcgtggc gatcctggag accgagaccg tggccgtcgg caccgcggcc
    13621 gccccttcga gcgatgtgaa tcctccgggt acctgggtgg ccggctcggg gcgcacgctg
    13681 gtcggcggaa actgggctat gacagcgccg tacatcgtca accctgggta cttcagtcct
    13741 cgcgccgaat gggaactctt cgaggcatcg gccgaccggc ggtggacgga catcacccgc
    13801 ac
//
LOCUS       DASRJA010000700         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_94995,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000700 DASRJA010000000
VERSION     DASRJA010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94995"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..613)
                     /locus_tag="VFC01_31650"
     CDS             complement(134..613)
                     /locus_tag="VFC01_31650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HZN84210.1"
                     /translation="MRPKTLVSTVGAVFATAALGGLASRPAESAWYARLKKPSFQPPR
                     QAFPIVWPILYADIAAVSASTLDHLHDHDHEQARLYAALLGANLVLNAGWTWLFFTRR
                     QLGVSALAAATLAASSADLTRRAVAARGSSGAVLGLYPAWCAFATLLSTRIWQLNRD"
     gene            700..1860
                     /locus_tag="VFC01_31655"
     CDS             700..1860
                     /locus_tag="VFC01_31655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN84211.1"
                     /translation="MTEILILGAGYTGMAATTGLVGRLKRRDDVHITLVNPQTRFTER
                     LRLHQTASGQALADLQIPDQLDGTGVDFVQGWVTGIDADAQTVRIDDTYTLRYDTLIY
                     ALGSVADTETVPGVDEFAYTLNSAQDATILADHLDRLADGTVVVAGGGLTGVESAAEI
                     AEQHPELDVVLLSRQVPGSMMGEKARPRLHAGLDRLGVQVHSGVEIVKVMSDGVALDD
                     GEVVDAQAVLWTTGVRVSPIAAAAGLEVDDRGRIVTDESLRSVTHPSVYAIGDAAAIH
                     QGYGVMHGTCQSGIPTALHAAASIVRELKGKQPKKFRFGYVHQPVSLGRNDAVIQFTH
                     ADDTPGRLYLAGPLAVAYKETVSSSPWTTYRLLKALPALGAATWRRGGGSTR"
     gene            1857..2750
                     /locus_tag="VFC01_31660"
     CDS             1857..2750
                     /locus_tag="VFC01_31660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HZN84212.1"
                     /translation="MNTDQQTFADHRNLLFSIAYRILGSAADAEDVVQDAWFKWSADD
                     RSQVSDPKAYLARIASNLSIERLRSTRHQRETYVGPWLPEPILTQPDAAEDVAKAESV
                     SMAMLVVLETLSPLERAVFVLKEVFDFSYAEIGDAVERSEAAVRQAAHRAREHVQARR
                     PRFETDRVKKREATERFFAATTGGDINALMDLLAPEVTLWTDGGGKVRQAMRPIIGAA
                     NVARWIAGTIKRPYEGVEIADMTAELVDINGGPGIVLSGTGRIIATLTVDLDADGRIV
                     TVHNVANPDKLHAVADGVKRI"
BASE COUNT          527 a          953 c          935 g          452 t
ORIGIN      
        1 gacgagtacc aacgcaatcg cccagtacac cgcgtcttcg aggaatagca gaactcggtc
       61 ggcgaaaagt tgacggtctt cttcgtcctc ttcgtcttct tgtgactgct ttttcctcgc
      121 caccgcggcc tcctcagtcg cggttgagtt gccagatacg ggtcgacaag agtgtcgcga
      181 acgcacacca cgccggatac agcccgagga cagcacctga ggaaccgcgc gcggcgaccg
      241 cccgtcgagt gagatctgcg ctgctggccg ccaacgtcgc tgcagccagc gctgacacac
      301 cgagttggcg acgagtgaag aacagccacg tccagccggc gttcaggacg aggttggcgc
      361 ccagcagtgc ggcatacaag cgggcctgct catgatcatg atcgtgaagg tggtccaggg
      421 tggacgccga taccgcggcg atgtctgcgt aaaggatcgg ccacacgatc ggaaacgcct
      481 ggcgcggcgg ctggaacgac ggcttcttca accgcgcata ccaggctgac tcggcgggcc
      541 ggctcgccaa accgcccaga gcagctgtgg cgaaaactgc tccaaccgtg ctgaccagtg
      601 tctttgggcg catgccgcta tttacccgca agtcgatgtc acgaatcgcg gccgtgcttg
      661 cgtctcgtgt gtatcagccg ccaaacgaaa ggcagaacga tgaccgagat cctgattctg
      721 ggcgccggat acaccggaat ggccgcgaca acgggcctgg tcgggcgcct gaaacgccgc
      781 gacgacgtcc acatcaccct ggtcaacccg cagacccggt tcaccgagcg actgcgcttg
      841 caccagaccg cctccggcca agctctggcc gacctccaga tccccgatca gctcgatggc
      901 acgggcgtgg acttcgtgca gggctgggtg accggcatcg acgccgacgc gcagacggtt
      961 cgcatcgacg acacctacac gctgcgctac gacaccctca tctacgcgtt gggcagtgtc
     1021 gccgatacgg agacagtgcc gggcgtcgac gaattcgcat acacgctgaa cagcgcgcaa
     1081 gatgcgacga ttctggccga ccatctggac cgactcgccg acggcacggt cgtggtggcc
     1141 ggcggcgggc tgaccggggt cgagtccgcg gccgagatcg ccgagcagca ccccgaactc
     1201 gacgtcgtgc tgctgagccg gcaggtgccc ggatcgatga tgggtgagaa ggcgcggccc
     1261 aggctgcacg cgggtctgga tcggctcggt gttcaggtgc attcgggcgt cgagatcgtc
     1321 aaggtgatgt ccgacggcgt cgcgctcgac gacggcgaag tcgtcgatgc gcaggcggtg
     1381 ttgtggacga cgggcgtgcg ggtctcgccg atcgctgccg ccgcgggcct cgaggttgac
     1441 gaccgtggcc gcattgtcac cgacgagtcc ctgcgatccg tcacgcatcc cagcgtctat
     1501 gccatcggtg acgcggccgc catccatcag ggctacggtg tcatgcacgg cacctgccag
     1561 agcggcatcc cgaccgcgct gcacgcggcg gcgtccattg tgcgcgagct gaaaggcaag
     1621 cagcccaaga agttccgctt cggttacgtc catcaaccgg tgagcctcgg ccgtaacgac
     1681 gccgtcatcc agttcaccca cgccgacgac accccaggac ggttgtactt ggccggcccg
     1741 cttgctgtcg cgtacaaaga gacggtgagc tccagcccat ggaccacata tcgactgctc
     1801 aaggcgctgc ccgcactagg agcggcgacc tggcggcgcg gcggcgggtc gacgagatga
     1861 ataccgacca gcagacgttc gccgatcacc ggaacctgtt gttctcgatc gcctatcgaa
     1921 tcctcggctc ggccgcggac gccgaagacg tcgtccagga cgcgtggttc aagtggtcgg
     1981 cggacgaccg ctcacaggtt tccgacccga aggcctacct cgcccgtatt gcgtcgaacc
     2041 tgtcgatcga acggcttcgc tcgacgcggc accaacgcga aacctatgta gggccatggc
     2101 ttccggagcc gatcctcacc cagcccgacg ccgccgagga cgttgcgaag gccgaatcgg
     2161 tgtcgatggc catgctggtt gtgctggaga ccctcagccc gttggagcgg gcggtgttcg
     2221 tgctcaagga agtgttcgac ttcagttacg ccgagatcgg tgacgcggtc gaacggtccg
     2281 aggccgcggt gcgtcaggcc gcgcaccgcg cccgcgagca cgtccaggcc cggcggcccc
     2341 gattcgagac cgaccgcgtc aagaagcgcg aagccaccga gcggttcttc gccgcgacga
     2401 cgggtggtga catcaacgcc ctgatggacc tgctcgcccc ggaggtcacg ctgtggactg
     2461 acggcggcgg caaggtgcgg caggcgatgc gcccgatcat cggcgcggcg aacgtggccc
     2521 gctggatcgc cggaacgatc aaacggccct acgagggcgt cgaaatcgcc gatatgacag
     2581 ccgaactcgt tgacatcaac ggcggcccgg gcatcgtgct gagcggtacc ggccgcatca
     2641 tcgccaccct cacggtcgac ctggacgccg acggccgcat cgtcaccgtc cacaacgtcg
     2701 cgaaccccga caagttgcat gcggtggccg acggagtcaa gcgcatctag cgtggcgtga
     2761 tgaccacgca atggcaggtg cgcaccggca gcgccgcgga cctggatctt gtgggcccgc
     2821 tgtgggtggc ggtccatcac cagcacaccg aaaccatgcc gcagctg
//
LOCUS       DASRJA010000701         5560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4044648,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000701 DASRJA010000000
VERSION     DASRJA010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4044648"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFC01_31665"
     CDS             complement(<1..292)
                     /locus_tag="VFC01_31665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84213.1"
                     /translation="MAADRLARWPAFPYDVTARLSLWAGVVIVAALWGWGAWERRWIA
                     DDGLIVLRTVRNLLAGNGPVFNAGERVESNTSTIWTYLIALGGWIGGPVRLEL"
     gene            complement(306..1220)
                     /locus_tag="VFC01_31670"
     CDS             complement(306..1220)
                     /locus_tag="VFC01_31670"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HZN84214.1"
                     /translation="MTEGPAPDKGPPRNIATGLVKAMRPRQWVKNLLVLIAPVAAFGS
                     DVKFDVGDVAIKALIAFVAFSLAASCIYLINDARDVEADRQHPTKRFRPIAAGVVPEW
                     LAYSVAVVLGIAALAVSFLATPNLAVVIAIYIVMQLAYCFGLKHQPVLDICIVASAYL
                     IRAIAGGAAANIPLSQWFLLGMTFASLFMVGGKRYAELQLAERTGAKIRKSLESYTAS
                     YLRFVWTLSATATVLCYGLWAFERDGNYASWYAVTIIPFTIAILRYAVDVDGGLAGEP
                     EEIALKDRVLQLLAVAWIGTIGAALFLS"
     gene            complement(1259..1810)
                     /locus_tag="VFC01_31675"
     CDS             complement(1259..1810)
                     /locus_tag="VFC01_31675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZN84215.1"
                     /translation="MAEPVREDDAAVPPRGEEAALIAVQHAVADRPGVLAGARALSHF
                     GEHSIGWVAVAALGALLQPRRRRAWLAAGVGAFVAHAGAVLIKRVVKRERPHHPAVAV
                     NVGTPSRLSFPSAHATSTTAASILLARTTGLPLPVLLVPPMALSRLVLGVHYPSDVLT
                     GVAVGAVVAKAVGAVADRVGGDG"
     gene            complement(1803..3740)
                     /locus_tag="VFC01_31680"
     CDS             complement(1803..3740)
                     /locus_tag="VFC01_31680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN84216.1"
                     /translation="MSDIPSGALDATESKAISLLARVILPRPGEPLDVRKLYIEESET
                     NARRAHAPTRTTLEIGGESEVSFATYFNAFPASYWRRWSTLDSVVLRVELTGSARVDV
                     YRSKATGARITVGGAPVSSGESEEPSAVEFEIDLSPFEDGGWIWFDITTNSKVTVHTA
                     GWYAPVPAPGRANVAVGIPTFNRPADCVNALAALTSDPLVDEVIGAVIVSDQGTKKAV
                     DHPGFAAASAPLGNRLTIHNQPNLGGSGGYSRVMYEALKNTDCEQILFMDDDIRIEPD
                     SILRALAMNRFAKVPTLVGGQMLNLQEPSHLHVMGEMVDSENFMWTNAVNTEYDHNFA
                     KYKLNDEDEYRSRLLHRRIDVDYNGWWMCMIPRQVAEELGQPLPLFIKWDDADYGLRA
                     GEHGYPTVTLPGVAIWHMAWSDKDDAIDWQAYFHLRNRLVVAALHWDGNVTGLIASHF
                     KATCKHLLCLEYSTVAIQNKAMDDFLAGPEHIFSILESALPDVRKMRQEYPDAVVLPS
                     ATALPTPSDKRWRKKVRIPTSPLAISFRLTRGVIHQLKAHDPEHHVRPQINVATQDAR
                     WFSLSRVDGVTVTTADGRGVVYRQRDRAKMFALLRESVRRQVRLARKFNRMRKVYREA
                     LPVLASKQKWETVLLESSANG"
     gene            complement(3737..4942)
                     /gene="glf"
                     /locus_tag="VFC01_31685"
     CDS             complement(3737..4942)
                     /gene="glf"
                     /locus_tag="VFC01_31685"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HZN84217.1"
                     /translation="MLPMTARFDLFVVGSGFFGLTIAERVATQLDKRVLVVDRRPHIG
                     GNAYSEPEPQTGIEVHKYGAHLFHTSNQRVWDYVRQFTDFTGYQHRVFAMHNGQAYQF
                     PMGLGLVSQFFGKYFTPDEARQLIKEQAAEINTEDAQNFEEKAISLVGRPLYEAFLKN
                     YTAKQWQTDPKELPASNITRLPVRYTFDNRYFNDTYEGLPADGYTAWLKNMAADDRIE
                     VRLDTDWFEVRDALRAENPDAPVVYTGPLDRYFDYAEGRLGWRTLDFEVEVLDTGDFQ
                     GTPVMNYNDGDVPYTRIHEFRHFHPERAYPTDKTVIMREFSRFADDDDEPYYPINTEA
                     DRALLAAYRARAKAETASSKVLFGGRLGTYQYLDMHMAIASALNMYDNTLAPHLRDGA
                     PLHDESTSA"
     gene            5110..>5560
                     /locus_tag="VFC01_31690"
     CDS             5110..>5560
                     /locus_tag="VFC01_31690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84218.1"
                     /translation="MPNRRRRRLSTAMSAVAALAVASPAAVIAVSDLADSTKPPTQHR
                     QFVQASMVTDLPNELMSALSQGLSQFGINLPPMPTSLLTGSGASTPTTLTSPGLGTSP
                     LTTPGLGTSPLTTPGLGTPPLTAPGGLGTTPLTAPSLGTDPAAAVPAL"
BASE COUNT          975 a         1835 c         1782 g          968 t
ORIGIN      
        1 cgagctccag acgcaccggt ccgccgatcc agccgccgag ggcgatcaga tacgtccaga
       61 tcgtcgacgt gttggactcg acacgctcgc ccgcgttgaa gaccggaccg ttgccggcca
      121 acaggttccg cacagtgcgc agcacgatca gcccatcgtc ggcgatccag cgccgctccc
      181 aggcgcccca cccccacagc gccgcgacaa tcactactcc ggcccagagg ctgagcctgg
      241 ccgtcacgtc atacgggaac gccggccagc gggccaagcg gtccgccgcc atattcctga
      301 cccgttcagc tgaggaagag ggcggcacca atggttccga tccacgccac cgcgagcagc
      361 tgcagcaccc ggtccttcag tgcgatctcc tccggctcgc ctgctaggcc accgtcgacg
      421 tcgacggcat accggaggat cgcgatcgtg aacggaatga tcgtaaccgc gtaccaggac
      481 gcgtaattgc catcccgctc gaaggcccac agcccgtagc aaaggacggt cgcggtcgcc
      541 gacagcgtcc acacgaaccg cagataggac gccgtgtagc tctccagcga cttgcggatc
      601 ttggcgccgg tccgctcggc gagttgcagt tcggcgtagc gcttgccgcc caccatgaac
      661 aacgacgcga acgtcatgcc aagcaggaac cattgggaca gcgggatgtt cgccgcggcg
      721 ccacccgcga ttgcccgaat caggtacgcc gacgcgacga tgcaaatgtc cagcaccggt
      781 tgatgtttca gcccgaagca gtatgccagc tgcatgacga tgtagatggc gatcaccact
      841 gccaggttcg gcgtggccag gaaggacacc gcgagggcgg cgatgccgag caccaccgct
      901 acggaatagg ccagccactc gggaaccacg cccgcagcga tcgggcggaa gcgcttcgtc
      961 gggtgctgcc ggtcggcctc gacatcgcgc gcatcgttga tcaggtagat gcaggaggct
     1021 gccaggctga acgcgacaaa tgcgatcaac gccttgatcg caacgtcccc cacgtcgaat
     1081 ttgacgtcgc tgccgaacgc ggccaccggc gcgatcaaca cgagcaggtt cttcacccac
     1141 tgccgaggtc gcatcgcctt gaccagaccc gtggcgatgt tgcggggcgg gcccttgtcc
     1201 ggtgcgggtc cttcagtcat gtcttgggcg ctctccgcgc tgctctcact cgagatgctc
     1261 atccatcccc tccaacccga tcagcgaccg ccccgaccgc cttcgcgacg accgcgccga
     1321 ccgcgacgcc ggtgagcacg tcgctgggat agtgcacacc aagcaccaag cgggacaacg
     1381 ccatcggcgg taccagcagc accggcaacg gtaggcccgt ggtgcgtgcc agcaagattg
     1441 acgctgcggt ggtcgacgtc gcatgcgcgg atgggaagct caaccggctc ggcgtgccga
     1501 cgttgaccgc gaccgccggg tgatgcggcc gttcccgctt gaccacccgc ttgatcaaca
     1561 ccgcgccggc gtgggccacg aacgcaccca cgccggcggc aagccacgcc ctgcggcgtc
     1621 ggggttgcag cagtgcaccg agcgcggcca cggcgaccca gccgatgctg tgctcgccga
     1681 aatgcgaaag cgcacgcgcg ccggcaagca cgccgggccg gtcggccacc gcatgctgaa
     1741 cggcgatcag cgcggcctcc tcaccgcgtg ggggcaccgc ggcgtcgtcc tcacggactg
     1801 gctcagccat tggccgacga ttccagaaga accgtctccc acttctgctt gctggccagc
     1861 accggcagag cctcgcggta gaccttgcgc atgcgattga acttgcgggc cagccgcact
     1921 tgccgtcgta ccgactcgcg aagcagcgca aacattttcg cgcggtcacg ctgccggtac
     1981 accacacccc ggccgtcggc ggtggtgacg gtgaccccgt cgaccctgga cagtgagaac
     2041 caccgggcgt cctgagtcgc gacgttgatc tgcgggcgca cgtggtgctc ggggtcgtgg
     2101 gccttgagct gatggatgac gccgcgggtc agccgaaacg agatcgccag tgggctggtg
     2161 gggatccgca ccttcttgcg ccaccgcttg tccgacggcg tcggtagtgc agtcgcactc
     2221 ggtagcacca ccgcgtcggg gtactcctga cgcatcttgc gcacgtcggg cagcgcggac
     2281 tccaggattg agaagatgtg ttccgggccg gccaggaaat catccatcgc cttgttttgg
     2341 atcgcgacgg tcgaatactc aaggcagaga aggtgtttgc acgttgcctt gaagtggctg
     2401 gcgatcaagc cggtgacgtt gccgtcccaa tgcagggccg cgacgaccaa ccggttgcgc
     2461 aggtggaagt aagcctgcca gtcgatcgca tcgtccttgt cgctccacgc catatgccag
     2521 atcgccacgc cgggcagcgt gaccgtggga tagccgtgct cacccgcgcg caacccgtaa
     2581 tccgcatcgt cccacttgat gaacagcgga agcggctgac cgagttcctc cgcgacctgc
     2641 cgcgggatca tgcacatcca ccagccgttg tagtcgacgt cgatgcggcg gtgcagaagc
     2701 ctgctgcggt actcatcttc gtcgttgagc ttgtacttgg cgaagttgtg gtcgtattcg
     2761 gtgttgaccg cgtttgtcca catgaagttc tcggaatcga ccatctcgcc catcacgtgc
     2821 aggtgcgacg gctcctgcag gttgagcatc tggccgccga cgagcgtcgg caccttcgcg
     2881 aaccggttca tcgccaacgc acgcaggatc gagtccggct cgatgcggat gtcgtcgtcc
     2941 atgaacagga tctgttcaca gtcggtgttt ttgagcgcct cgtacatcac ccggctgtag
     3001 ccacccgagc cgccgaggtt cggctgattg tggatcgtca gacggttgcc cagcggggcc
     3061 gaggccgcgg cgaacccggg gtggtcgacg gccttcttgg tcccctgatc cgacacgatt
     3121 accgcaccaa ttacctcgtc caccaacgga tccgaggtca gcgcggccag cgcgttgacg
     3181 cagtcggcgg gccggttgaa ggtcgggatg ccgactgcga cgttggcgcg gcccggcgct
     3241 ggcaccggcg cataccagcc ggcggtgtgc acggtgacct tggaattggt ggtgatgtcg
     3301 aaccagatcc agccgccgtc ctcgaacggt gataggtcga tctcaaactc gacggctgac
     3361 ggttcctcag attccccgct ggagactggg gcgccgccaa cggtgatccg cgcacccgtg
     3421 gccttggacc ggtagacgtc gacgcgggca ctgccggtca actcgacgcg aagcacaacc
     3481 gagtcgagcg tcgaccaccg ccgccaatag ctggcgggga aggcgttgaa gtacgtcgcg
     3541 aacgaaacct cggactcccc accgatttcg agcgtcgtgc gggtcggcgc atgcgcacgc
     3601 ctggcgttgg tttccgattc ctctatatat agcttgcgga cgtcgagcgg ttcgcccggc
     3661 cgaggcagga tgactcgggc gagaagactg atcgccttgg attccgtggc gtcgagcgcg
     3721 ccggacggga tgtcgctcat gcgctggtgc tttcgtcgtg gagtggcgcg ccgtcgcgaa
     3781 ggtgcggcgc gagggtgttg tcgtacatgt tcagtgcgct ggcgatggcc atatgcatgt
     3841 cgaggtactg gtaggtcccc agccggccac caaatagcac cttcgatgat gccgtctcgg
     3901 ccttggcccg cgcacggtac gcggccaaca aggcccggtc ggcttcggtg ttgatcgggt
     3961 agtagggctc atcgtcgtcg tcggcgaacc gggagaactc ccgcatgatc accgtcttgt
     4021 cggtcggata cgcccgctcg ggatggaagt gccggaactc gtggatgcgg gtgtagggca
     4081 cgtcgccgtc gttgtagttc atcacagggg tgccttggaa atcaccggtg tcgagcacct
     4141 cgacctcgaa atcgagtgtg cgccagccca accgaccctc ggcgtagtcg aagtagcggt
     4201 ccagcggccc ggtgtagacc accggagcgt cggggttttc ggcgcgcagc gcgtcgcgga
     4261 cctcgaacca gtcggtgtcc agtcgcacct cgatgcggtc gtcggcggcc atgttcttca
     4321 gccacgcggt gtagccgtcg gcgggcagcc cttcgtaggt gtcgttgaag tagcggttgt
     4381 cgaacgtgta gcgcaccggc agccgtgtga tgttgctcgc cggcagttcc ttgggatcgg
     4441 tctgccactg ttttgcggtg tagttcttga gaaacgcctc gtacagtggg cgcccgacca
     4501 ggctgatcgc cttttcttcg aagttctgcg cgtcttcggt gttgatctcg gcggcctgct
     4561 ccttgatcag ctggcgagcc tcgtcgggcg tgaagtactt gccgaagaac tgcgacacca
     4621 ggccaaggcc catcgggaac tggtaggcct ggccgttgtg catcgcgaag acgcggtgct
     4681 ggtaaccggt gaagtcggtg aactgccgca cgtagtccca cactcgctgg ttgctggtgt
     4741 ggaacaggtg cgcgccgtac ttgtgcactt cgataccggt ctgcggctcg ggttcggagt
     4801 aggcgttgcc gccgatatgc gggcgtcggt cgacgacgag aacgcgcttg tccagctgtg
     4861 tggccacgcg ctcggcaatc gtcagaccga aaaatccgga acctacgaca aagaggtcaa
     4921 aacgagcggt catcggcagc aagggtatcc gaccgcgcct gctcgccccg aatccaacga
     4981 gagcgcaagg ttgcgatcgg ctcacaattc cgtatcgcca cttctacctc atcagtcaca
     5041 ccagtaccgt cgatctcgtt acacccaaga gcacgtacca gacgtagtcc atatattgag
     5101 gagacttccg tgccgaaccg acgtcgccgc aggctttcga cagccatgag cgcagtcgcc
     5161 gccctggcag tcgcgagtcc agcagcagta atcgccgtgt ccgacttggc cgacagcacc
     5221 aagccgccga cgcagcaccg ccagttcgtc caggcgtcaa tggtcaccga cctgcccaac
     5281 gaactgatgt cggcgctgtc gcaggggctg tcgcagttcg ggatcaacct gccgccgatg
     5341 ccgacgagtc tgctcaccgg ttcgggcgcg agcactccga cgacgctgac ttcaccgggc
     5401 ctgggaacat cgcccctgac cacaccgggg ctgggaacat cgccgctgac cacccccggg
     5461 ttgggaacgc caccgctgac cgcacccggc ggcctgggta caactccgtt gactgcaccc
     5521 agccttggca ccgacccggc ggccgccgtt cccgcgctcg
//
LOCUS       DASRJA010000702         8337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2550014,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000702 DASRJA010000000
VERSION     DASRJA010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550014"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFC01_31695"
     CDS             complement(<1..432)
                     /locus_tag="VFC01_31695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZN84219.1"
                     /translation="MRWLALVTALALVCAPAAWAGDGRLDWPLRPRPAVLRTFDAPSP
                     NWNRGHRGVDLAGVPGQPVYAAGAGTVVFAGELAGRTLVSIAHSGGQRTTYEPVRPSV
                     RVGQLVDAGAVIGELEAGHDGCAAAACLHWGAMWGPASRADY"
     gene            713..1546
                     /gene="rpsB"
                     /locus_tag="VFC01_31700"
     CDS             713..1546
                     /gene="rpsB"
                     /locus_tag="VFC01_31700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZN84220.1"
                     /translation="MAVVTMKQLLDSGTHFGHQTRRWNPKMKRFIFTDRNGIYIIDLQ
                     QTLTYIDKAYEFVKETVAHGGSIMFVGTKKQAQESIAEEATRVGMPYVNQRWLGGMLT
                     NFSTVHKRLQRLKELEAMEQTGGFEGRTKKEILMLTREKNKLERSLGGIRDMQKVPSA
                     IWVVDTNKEHIAVGEARKLGIPVIAILDTNCDPDLVDYPIPGNDDAIRSAALLTKVIA
                     SAVAEGLQARAGVGKGDGEAEAEGAEPLAEWEQELLAGATATATAETPAAETNTETTE
                     G"
     gene            1548..2363
                     /gene="tsf"
                     /locus_tag="VFC01_31705"
     CDS             1548..2363
                     /gene="tsf"
                     /locus_tag="VFC01_31705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZN84221.1"
                     /translation="MANYTAADVKRLRELTGAGMLDSKNALVEADGNFDKAVELLRIK
                     GAKDVGKRAERATAEGLVAAKDGALIELNSETDFVAKNAEFQAVADQIVAAAAAAKAN
                     DVDALKAAKIGDTTVEQTIADLSAKIGEKLELRRAAYFDGTVETYLHKRAADLPPAVG
                     VLVEYTGSDKDAAHAVALQIAALKAKYLTREDVPEDVVANERRIAEETARNEGKPEQA
                     LPKIVEGRVTGFYKDVVLLDQPSVSDNKKTVKALLDAAGVTVTRFVRFEVGQA"
     gene            2403..3806
                     /locus_tag="VFC01_31710"
     CDS             2403..3806
                     /locus_tag="VFC01_31710"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN84222.1"
                     /translation="MSRIHAFGDDALGDLDAVGLVEKLRGGEVSPQELVEAAIARTEA
                     VNPTLNGLAYQGFGRARTRASARGYGGFFDGVPSFIKDNVAVAGMPTMEGTDAWQPNP
                     ALLDGDFARSYLATGLMPLGKTQLSEFGFSASAEHPRLGPVRNPWNPDHTAGASSSGS
                     GAFVAAGVVPIAHANDGGGSIRIPASCNGLVGLKPSRGRLPLDKAHRQMPLRIVANGV
                     VTRSVRDTAAFYREMERVSRNPKLPAIGDITHPGKQRLHVAVCTQSIAREASPEIREL
                     TLKSAALLEELGHKVTQIDNPVPPQFMDDFLLYWSFLAFALVRGGRRTFGASFDRTRL
                     DNLTLGLERHSGSRLHRLPLALARLSRVRRLTSRLSNTYDVVLTPTLADEPPRIGHLD
                     PTVDYQQIIDRLIEWVAFTPLQNATGEPAISLPLAESAAGLPIGMMFAAPIGQEARLL
                     ELAYELEDARPWAQIHP"
     gene            3803..4567
                     /locus_tag="VFC01_31715"
     CDS             3803..4567
                     /locus_tag="VFC01_31715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN84223.1"
                     /translation="MTRDYILNQGFADERKRLAGMESLWDPGSQALLDELGIGGGWRC
                     LEVGAGGGSMVQWMADRGASVLAIDIDTRFVDSLASDAIEVRRVDLRTDPLPQDEFDL
                     VHSRLVLEHLSDRRQILDRLAATLRPGGWMVIEDYDWTCFGFEGESPGFSDIAEVILG
                     FMAKAGFERDYGRRVVADIEAAGLTDVRGEGRARLIGPSSPGFDFFRLTFESLRGAIV
                     DAGLLSAEEADAAAEQFNEDVRLLTPLMMAGIGRRA"
     gene            4688..5851
                     /locus_tag="VFC01_31720"
     CDS             4688..5851
                     /locus_tag="VFC01_31720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN84224.1"
                     /translation="METSAPTTVRSPEIATASGNTFVCLVRFALANIRRRPERFVLSV
                     LGIALAIACVTVVRTISASFAITGADSVTDVLGGAQLWAVPAAGVHYDSNAQALVADG
                     AAPVITLPAGWTATRVLSGVTDINGSTVSLRGTDEIPSDQAILGADLASRLDLQPGDR
                     LVVGGQRLVVTVVGSGQSITVTTPLALSIVGDNGWWTIFAPAGDEKRRDLGQIFGGAV
                     GLESTSDPSVKPDPNGHGLIYDTVGGSGPLTFEQKFSALFSAKVTGSTLGLISTIGLG
                     LGFVIAVSSFLAAVQERRREFGIMSSIGLADEVLYFFLVESGIVFITAYLVGVIGAGA
                     AVALVIPGIATPTAWLQASGMVAAFLPAMAIVGALIPVHRLLQQRPVSLLGDH"
     gene            5851..6417
                     /locus_tag="VFC01_31725"
     CDS             5851..6417
                     /locus_tag="VFC01_31725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN84225.1"
                     /translation="MLSRGLSYGWLAARRRIREMVLPVVTTATGAFLVVIVFGMSDGI
                     RAQSASLGHAEEISRAVVLIAVTVLLVGVVEVAVATTRTVAHRTRELGVLGANGVPRT
                     PVVAALLVEPVIAAVLGALAGALLAAVTGIVLGAAGLVATGVSVEGLMLGAAISVGVS
                     IVAALATSIVPTWNAASRPPIRSLTAGG"
     gene            6420..7142
                     /locus_tag="VFC01_31730"
     CDS             6420..7142
                     /locus_tag="VFC01_31730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN84226.1"
                     /translation="MTAIDETAATTIHTDEPRPVIEISDVWKLHKLGDEVVRALVAAE
                     LQVMPGEFVCLMGPSGSGKSTLLNIIGGLDRPTKGTVKLAGRDTSLLTESQFAALRHD
                     TIGFIFQSYNLIPFLSAVENVELPLMFEPYDRKSLRKRALELLELVGLGHRIHHQPTK
                     MSGGEQQRTAIARSLISSPTLVLADEPTANLDHRTGETVVRMLRDLCSTLGVTVVAST
                     HDPTVADEASRVVRMKDGQIVN"
     gene            7167..>8337
                     /locus_tag="VFC01_31735"
     CDS             7167..>8337
                     /locus_tag="VFC01_31735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN84227.1"
                     /translation="MTQELEAAPPSSQRDALMTTELVPEQVLPKVLGTFGLTATYVFI
                     ICWITGSSVMAGGGWTAIPMWILGIITFLVPAALAVAELGNLWPGQGGVYIWAFRTMG
                     ERWSFLGGYLSWVPVVLNTASSPAIVLQFMLLAFHAELGLTVSIVVQLVILWTVVGLA
                     LAKLAANQRIMNITFVVYGVLTLTIFICGVIYAAGHGSATPFSLHDSLVPNFAGAGFL
                     YGTVLLYLVGVETPFNMGAEFLSVKRSATKMITWGSAALIAIYLLTTLGTMMVLPGDQ
                     IDPVTGVIGNLGTSGFPGLMEVCAVILAVIVFVAMTTYQVAYSRLIFVSGLERHLPRI
                     FTHLNPRTRNPVTAVLIQGVLSSLILVGLYSQSSMANVTVYLQGGLSVVWLISGFF"
BASE COUNT         1400 a         2787 c         2719 g         1431 t
ORIGIN      
        1 gtagtccgcg cgcgaggccg gaccccacat cgccccccaa tgcaggcacg ccgcggcggc
       61 gcaaccgtca tgtcccgcct ccagctcgcc gatcaccgcc ccggcgtcaa cgagctgacc
      121 aacgcggacc gacggtcgca ccggctcata cgtcgtccgc tgcccgccgg aatgcgcgat
      181 cgataccagc gtccgcccgg ccagctcccc ggcgaacacc accgtgcccg cgccggccgc
      241 atataccggc tgccccggca ccccagccag gtcgacgccg cgatggccgc ggttccagtt
      301 cggcgacggc gcgtcgaacg tccgaagaac agctggtcgg ggccgcagag gccagtccag
      361 cctgccgtcg ccggcccatg ccgccggcgc gcagaccagt gcgagggccg ttaccaatgc
      421 cagccagcgc atacccccag ctcagcgcgt cgacgccaca gaatgccagt ccacgccgcc
      481 aagctgtgga tggcggccgc ggtgtggaaa aagcacccct atccagcgtg taaactgcta
      541 accgcagctc gctagagcgg gctgacttcg cgtgtctgca ccacgacccg tttttctagg
      601 tcgtatccgg atgccgtcgg tcccgtccca gacgggtcgg cactccagca ggcaccaggg
      661 ctcggcgtca cccggcgccg cgcgtcaacc gacaacaaaa gaggcaataa ccatggccgt
      721 agtgaccatg aaacagctgc tggacagcgg cacccacttc gggcatcaga cccgtcgctg
      781 gaatcccaag atgaagcggt tcatcttcac cgaccgcaac ggcatctaca tcatcgatct
      841 gcagcagacg ctgacctaca tcgacaaggc gtacgagttc gtcaaagaga ctgtcgctca
      901 cggcggcagc atcatgttcg tcggcaccaa gaagcaggct caggaatcca tcgccgaaga
      961 ggcgacccgc gtcggcatgc cctacgtgaa ccagcgttgg ctgggcggca tgctcaccaa
     1021 cttctccacc gtgcacaagc ggctgcagcg cctcaaggaa ctcgaggcga tggagcagac
     1081 cggtggcttc gagggtcgca ccaagaaaga gatcctgatg ctgacgcgcg agaagaacaa
     1141 gctcgagcgc agcctcggcg gtatccgcga catgcagaag gtgccgtcgg ccatctgggt
     1201 cgtcgacacc aacaaggagc acatcgccgt cggcgaggcc cgcaagctgg gcatccccgt
     1261 catcgcgatc ctcgacacca actgcgatcc agacctcgtc gactacccga tcccgggcaa
     1321 cgacgacgcg atccgctcgg ccgcgctgct caccaaggtg atcgcgtccg cggtcgccga
     1381 gggcctgcag gcccgcgctg gcgttggcaa gggcgacggc gaggccgagg ccgagggcgc
     1441 cgagccgctc gccgaatggg agcaggagct gctggccggg gccaccgcca ccgccaccgc
     1501 cgagaccccc gccgccgaaa ccaacaccga gaccacggaa ggctgaaatg gctaactaca
     1561 ccgctgccga cgtcaagcga cttcgggagc tgaccggcgc cggcatgctc gattcgaaga
     1621 acgcgttggt tgaggccgac ggcaacttcg acaaggccgt cgagctgctg cgcatcaagg
     1681 gcgccaagga cgtcggcaag cgggccgagc gtgccaccgc cgagggcctg gtcgccgcca
     1741 aggatggcgc gctgatcgaa ctcaactccg agaccgactt cgtcgcgaag aacgcggagt
     1801 tccaggcggt cgccgaccag atcgtcgcgg ccgctgccgc cgccaaggcg aatgacgtcg
     1861 acgcgttgaa ggccgccaag atcggtgaca ccaccgtcga gcagacgatc gctgacctgt
     1921 ccgccaagat cggcgagaag ctcgagctgc gtcgcgccgc ctacttcgac ggcaccgtcg
     1981 agacctactt gcacaagcgg gccgcggacc tgccgcccgc ggtcggtgtg ctggtcgagt
     2041 acaccggctc ggacaaggac gccgcgcacg cggtcgccct gcagatcgcg gcgctgaagg
     2101 ccaagtacct cacccgtgag gacgtgccgg aagatgtcgt cgccaacgag cggcgcatcg
     2161 ccgaggagac cgcccgcaac gagggcaagc ccgagcaggc gctgcccaag atcgtcgagg
     2221 gtcgcgtgac cggcttctac aaggacgtcg tgctgctcga tcagccgtcg gtgtccgaca
     2281 acaagaagac cgtcaaggcg ctgctcgacg cggcaggcgt gaccgtcacc cggttcgtcc
     2341 gcttcgaggt cggccaggcg taaccctcgt gctccggcgg tacgacttgg taccgtcggt
     2401 gcatgagtcg catccacgcc ttcggcgacg acgcccttgg cgaccttgac gctgtcggtc
     2461 ttgtcgagaa gctccgtggt ggcgaggtgt cgccgcagga gctcgtcgag gccgccatcg
     2521 cccgcaccga agccgtcaac ccgacactga acgggctggc gtaccagggg ttcggccgcg
     2581 cccgaacccg ggcgtcggcg cgcggttacg gcggtttctt cgacggcgtg ccgtcgttca
     2641 tcaaggacaa tgtcgccgtc gcgggtatgc cgacgatgga gggcaccgac gcgtggcaac
     2701 ccaacccggc actgctcgac ggcgacttcg cacggtcgta cctcgcgacc ggcctgatgc
     2761 cgctgggcaa gacgcagttg tccgaattcg gtttcagcgc ttcggccgaa cacccgcgtc
     2821 tcggcccggt ccgcaacccg tggaatcccg accacaccgc cggtgcgtcg tcgtcgggat
     2881 cgggtgcgtt cgtcgcggcg ggcgtggtgc cgatcgcaca tgcgaacgac ggcggcgggt
     2941 ccatccgaat ccccgcttcc tgcaacggat tagtgggcct caagccgtca cggggccggc
     3001 tcccactgga taaggcacac cggcagatgc cgctgcggat cgtcgccaac ggtgtcgtga
     3061 cgcgctcagt gcgcgacacc gcggccttct accgggagat ggagcgggtg tcgcgaaacc
     3121 cgaagctgcc ggccatcggc gacatcaccc accccggcaa gcagcggctg cacgtagccg
     3181 tgtgcacgca atccattgcg cgggaagcca gcccggagat tcgggagctg acgctcaagt
     3241 ccgccgcact gctggaggaa ctgggtcaca aagtcactca gatcgataac ccggtgccgc
     3301 cgcagttcat ggacgacttc ctcctgtatt ggtcgttcct ggcgttcgcc ttggtacgcg
     3361 gtgggcgccg cacgttcggc gcgagcttcg atcgcacgcg gctggacaac ctgacgctgg
     3421 gcctggagcg ccattctgga agcaggcttc atcggctgcc gctggcgctg gcgcggctgt
     3481 ctcgggttcg ccggctcacg tcgcggctgt ccaacaccta cgacgtcgtg ctcacaccta
     3541 ccctggccga cgagccgccg cggatcggac atctcgatcc caccgtggac taccagcaga
     3601 tcatcgacag gctcatcgag tgggtggcgt tcacaccatt gcagaatgcg accggagagc
     3661 ccgcgatctc gctgccgctt gcggaatccg cagccggact gccgatcggg atgatgttcg
     3721 ccgcgccaat cggtcaggaa gcccgactgc tggagcttgc ctacgaactc gaagacgccc
     3781 ggccgtgggc gcagattcac ccatgacccg cgactacatt ctcaaccaag gtttcgccga
     3841 tgagcgaaag cggttggccg gcatggaatc tctgtgggat cccgggtcgc aggcgctgct
     3901 cgacgaactc ggcattggcg gcggttggcg ctgcctcgag gtcggcgccg gcggcggatc
     3961 gatggtgcaa tggatggcgg accgcggcgc cagcgtcctg gcgatcgaca tcgatacccg
     4021 attcgtcgat tcgctagcct cggatgcgat cgaggtacgc cgcgtcgact tgcgtactga
     4081 tccgctgcca caggacgagt tcgatctcgt tcactctcgg ctagtgctcg agcacctctc
     4141 tgaccgacgt cagatcctcg accgcctggc cgcgacgctc cgccccggcg gatggatggt
     4201 catcgaggac tacgactgga cgtgcttcgg cttcgagggt gagagcccgg gattcagcga
     4261 tatcgccgaa gtcattctgg gtttcatggc gaaggccgga ttcgaacggg actatgggcg
     4321 ccgtgtggtc gctgatattg aggccgcggg actcaccgac gttcgcggcg agggtcgggc
     4381 ccggctgatc ggcccgtcgt cacctggctt cgatttcttt cggttgacct tcgagagcct
     4441 tcgtggagcc atcgtggacg cagggctgct gtcggccgag gaagctgacg ccgcggcaga
     4501 gcaattcaat gaggacgtcc ggctactcac cccgttgatg atggccggaa tcggccgccg
     4561 cgcctgaccc gacaacgaaa tccgttgcgt cggtgcaata gcgctgtaac gcgagcacgt
     4621 ccgagttaac gccgtggaca ttccgcgcag cggtgcgtga aactttccgg ccccaccatc
     4681 cctgtttgtg gagacatctg cgcccacaac cgtccggtca ccggagatcg ccaccgcctc
     4741 gggcaacaca tttgtgtgcc tcgtccggtt cgcgctggcg aacatccgcc gtcgtcccga
     4801 acggttcgtg ttgtccgtgc tcggcatcgc gcttgcgatc gcgtgcgtca ccgtcgttcg
     4861 gacgatctcg gcgagtttcg cgatcaccgg cgccgattcc gtcaccgacg tgttgggtgg
     4921 cgcccaactg tgggcggtgc ctgccgctgg cgtgcactac gacagcaatg cccaggccct
     4981 tgtcgccgac ggtgccgcgc cggtcatcac ccttcctgcc ggctggaccg cgacccgtgt
     5041 cttgtccgga gtcactgata tcaacggcag tacggtgtcg ctgcggggaa ccgacgaaat
     5101 acccagtgat caagccattt tgggcgctga cctagccagc cggctcgacc tgcagcccgg
     5161 cgaccggctc gtggtgggcg ggcagaggct cgtcgtgacg gtggtaggca gtggccagtc
     5221 gatcaccgtg accacaccgc ttgccctgtc gatcgtcggt gacaacgggt ggtggacgat
     5281 cttcgcgccg gccggcgacg agaagcgccg cgacctcggg cagatcttcg gcggcgcggt
     5341 cggactggag tcgacgtcgg acccgtcggt caagccggat cccaacgggc acggcctcat
     5401 ctacgacacc gtcggcggct ccgggccgtt gaccttcgag cagaagttct cggcgctgtt
     5461 ctccgccaag gtgacgggct cgacgctcgg cctgatctcg accatcggtc tcggcctcgg
     5521 tttcgtgatc gcggtgtcgt cgttcctggc cgctgtccag gaacgaaggc gcgagttcgg
     5581 catcatgtcc agcatcgggc tggccgacga agtgctttac ttcttcctcg tcgagtcggg
     5641 gatcgtcttc atcaccgcgt atctggtcgg cgtgatcggc gcgggagccg ctgtcgcgct
     5701 ggtgattccc ggcatcgcca caccaaccgc gtggctgcag gcctcgggca tggtcgcggc
     5761 gttccttccc gcgatggcga tcgtcggcgc cctgattccg gtgcaccggc tgctccaaca
     5821 acgtccggtt tcgctcctgg gggaccactg atgctaagta gaggtttgtc ctatggatgg
     5881 ctggcggcgc gccggcggat ccgcgagatg gtgctgcctg tcgtcacgac cgccacgggt
     5941 gcgtttctgg tggtgatcgt gttcggcatg tccgacggga tccgcgcaca gtcggcgtcg
     6001 ctgggccacg cggaggagat cagccgcgcc gtcgtgctga tcgcagtgac cgttctgctg
     6061 gtgggcgtgg tcgaggtggc cgtggcgacc acccgcaccg tcgcccatcg cactcgcgaa
     6121 ctcggggtgc tcggcgccaa tggtgtgccg cgcacacccg tggtggccgc gctgctggtg
     6181 gagccggtga tcgcggcggt gctgggcgcg ctggccggtg cgctactggc cgcggtgacc
     6241 gggatcgtgc ttggcgcagc tggtttggtc gcgacgggag tgtcggtcga ggggctgatg
     6301 ctcggcgcgg cgatctcggt cggcgtcagc atcgtcgcgg cgctggccac cagcattgtg
     6361 cccacctgga acgccgcctc gcggccaccg atccgctccc tgaccgcagg aggctagaga
     6421 tgaccgcaat cgacgaaacc gcagccacca cgattcacac cgacgaaccc aggccggtga
     6481 tcgagatctc ggatgtgtgg aagctgcaca agctcggcga tgaggtggtc cgtgcgctcg
     6541 tcgccgccga attacaggtg atgccaggtg aattcgtgtg cttgatggga ccgagcggca
     6601 gcggcaagtc gaccctgctc aatatcatcg gtgggctcga ccgaccgacc aagggaacgg
     6661 tgaagctcgc ggggcgcgac acgtcgctgc tgaccgagag ccagttcgcg gcgctgcgcc
     6721 acgacaccat cgggttcatc ttccagagct acaacctgat tccatttctg tcggcggtgg
     6781 agaacgtcga actgccactg atgtttgaac cctacgaccg gaagtcgttg cgcaagaggg
     6841 cactcgaact actcgagctc gtcgggctcg gacatcggat tcaccaccag ccgacgaaga
     6901 tgtcgggcgg agaacagcag cgcaccgcga tcgcacggtc gctgatcagt agcccaacgc
     6961 tcgttctggc cgacgagccg acggccaatc tcgaccaccg caccggggag acggtggtgc
     7021 gcatgttgcg cgacctgtgt tccacgctgg gcgtcaccgt tgtcgccagc acgcacgacc
     7081 ccacggtggc cgacgaagcg agccgtgtcg tccggatgaa agacggacaa atcgtcaact
     7141 gacccaacgg cattgggaga gtcattatga cccaagaact cgaggcggcc ccgccgtcgt
     7201 cacaacgcga cgcgctcatg acaaccgagc tcgtccccga gcaggtgttg cccaaggtcc
     7261 tgggcacctt cggactgacc gccacctacg tcttcatcat ctgctggatc accgggtcgt
     7321 cggtgatggc cggtggtggc tggacagcga tcccgatgtg gatcctcggc atcatcacct
     7381 tcttggtgcc cgccgccctg gcggtcgccg aattggggaa cctgtggccc ggccaaggtg
     7441 gcgtgtacat ctgggcgttc cggaccatgg gggagcgatg gtcgttcctc ggcggttacc
     7501 tgtcctgggt gcccgtcgtt ctgaacaccg cgtcctcgcc tgcgatcgtg ctccagttca
     7561 tgctcctggc cttccacgcg gagctcgggc tcacggtgag catcgttgtg cagctggtga
     7621 tcctgtggac agtcgttggg ttggcgctcg cgaaactggc cgccaatcag cggatcatga
     7681 acatcacgtt cgtggtctac ggcgtgctca ccttgacgat cttcatctgc ggtgtcatct
     7741 acgccgccgg gcacgggtcc gcaaccccat tcagcctgca cgactcgctg gtgccgaact
     7801 tcgcgggcgc tggcttcctg tacggcacgg tgctgctcta cctggtcggc gtcgagacgc
     7861 cgttcaacat gggcgcggag ttcctgtcgg tcaagcgcag cgccaccaag atgatcacgt
     7921 ggggatcagc ggcgctgatc gcgatctacc tgttgacgac gctcggcacg atgatggtgc
     7981 tgccgggcga ccagatcgac ccggtcaccg gtgtcatcgg aaacctgggc acctccggct
     8041 tccccggtct gatggaagtc tgcgctgtga tcctcgctgt catcgtcttc gtggccatga
     8101 cgacctatca ggtggcgtac tcgcggttga tcttcgtctc cgggctggag cgccatctgc
     8161 cgcgaatctt cactcacctc aacccgcgga cccgcaaccc ggtcaccgcg gtgctgatcc
     8221 agggcgtgct gtcgtcgctg attctcgtcg gcctgtactc gcagagcagc atggccaacg
     8281 tgacggtgta tctgcagggt ggcctgagcg tcgtgtggct gatctccgga ttcttct
//
LOCUS       DASRJA010000703         4470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2794569,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000703 DASRJA010000000
VERSION     DASRJA010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2794569"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFC01_31740"
     CDS             complement(<1..332)
                     /locus_tag="VFC01_31740"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HZN84228.1"
                     /translation="MLTAAVAALNRGEMIVVEDDRARTNEGDLVMAAARMTDQQMAFY
                     LRYGSGIVCVPMTEQRADALDLPLMTQRNSDPDGTAFTISADHVATGTGISAADRALT
                     VRTLAGATT"
     gene            complement(326..1171)
                     /locus_tag="VFC01_31745"
     CDS             complement(326..1171)
                     /locus_tag="VFC01_31745"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZN84229.1"
                     /translation="MALAFDIVGKRFGPTAVSWTEADAQIYALGVGAGAPDPLVDLEF
                     TTECAELGKQRVLPTFAIGLMQQHGLEIDLGDVSASQRLHAEQSLVMLAPLAASGTVH
                     VTRRVTDIWDKRSGALVNFESTAVDPVTDEPIAIAVSRSFIRGEGGFGGDSGPSAPSP
                     IPDRAPDLEIEMATMPNQALLFRQAGDRNPLHVDPDVAQRGGFARPILHGLCTYGFAA
                     RALLRHVCDNDPARFGRITGRFSAPTYPGDVLTVAMWLSDRTASYQVRSGATVVIDRG
                     EFHAC"
     gene            complement(1172..1870)
                     /locus_tag="VFC01_31750"
     CDS             complement(1172..1870)
                     /locus_tag="VFC01_31750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008263165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN84230.1"
                     /translation="MSEPWPVAGVPAPTAGSGTIRERVCRVLRERILTGVLAPGARID
                     LDAVAVEFDTSRTPVREACLELALDGLVRVAPRTGIIVLGATPEAILENFALMAALLG
                     VAAEWAAQRITPEQLKRVRALKVEVAIAMRSGDDAATENWLFHREVNKACGSPRLLAM
                     LGMAGRMIPHGFLDALPEDVPCSLEEHDALVRALANHDGAEARRVTEKHLQRAAEQLR
                     AKVSELEVQAEAGA"
     gene            1966..2868
                     /locus_tag="VFC01_31755"
     CDS             1966..2868
                     /locus_tag="VFC01_31755"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZN84231.1"
                     /translation="MTQTTRPLTTVAPLRSLLFCPATSARRVRKMLELAPDAAVLDLE
                     DAVTDDNKTAARQQARAGIAEIAPIRSYVRVNHPSTGRTQGDIDAVVQPALDGIVLPK
                     VEDVDTIQDADGWITAAERQNGVEPGCTRLLVLIESARGIADAREILQASARIETAVF
                     GAHDYMLDLGIDGLDHSESAEELLYARSALVIAARAARVSQPLDGPFIAFGDTDRFVE
                     MCRQARRMGFRGKMLIHPNQVALSHLGFAPTDAEIATANRMIEQFAEAEAEGAAAIVV
                     DGRLVDYPIVARARRIIEAAAELQ"
     gene            2865..4058
                     /locus_tag="VFC01_31760"
     CDS             2865..4058
                     /locus_tag="VFC01_31760"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN84232.1"
                     /translation="MTRSLDGIRVIDAASLAAGPLVATYLGELGAEVIKIEQPGTGDA
                     IRSWGAQKNGHGLMWKSVGRNKRTATLDLRKPAGQQLFCELAAVADVVIINTRPSTLE
                     RWGLSYESLATVNPGLVMLHVTGFGAGGPNSDRPGFGTLGEAMSGFAHTTGEQDGPPT
                     LPSFMLADGVAALTGTYAVLAALYHRDMHGGTGQLIDLNLVEPLARLLEQSVLTYDQL
                     GIVPTRAGNKWDISAPRNTYRTKDDQWIAVSGSAPTVAKRIFKAIGRAELADDPDFGD
                     PQQRLKNADAVDAAVAEWIIARTLDEAMTVFELEEVAAAPVYDAPRLLADPHLQARKT
                     YAPVDDPDLGSIRMQAPVPRLSQTPAVIDHTGRGLGADNAYVYGEILALTEEEIERLR
                     SDNVI"
     gene            4192..>4470
                     /locus_tag="VFC01_31765"
     CDS             4192..>4470
                     /locus_tag="VFC01_31765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003877980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZN84233.1"
                     /translation="MALLLRPRRSALYLPGNNSRALEKAKTLSADVLIFDLEDAVGPD
                     AKADSRTRVIEAISSGSYRPRELVVRVNGSGTVWHDDDLAAVARSAADG"
BASE COUNT          845 a         1505 c         1351 g          769 t
ORIGIN      
        1 gtggtggcgc cggcgagcgt ccttaccgtc agcgcgcggt cggccgccga gatacctgtc
       61 cccgtcgcga cgtgatcggc gctgatggtg aacgccgtcc cgtccggatc ggagttgcgc
      121 tgcgtcatca gcggcaggtc cagtgcgtca gccctttgct ctgtcattgg tacacagacg
      181 attccgctgc cgtagcgaag ataaaatgcc atttgttggt cggtcattcg ggcggctgcc
      241 ataacgaggt caccctcatt ggttcgagca cgatcgtctt cgacgacgat catctcgccg
      301 cggttgagcg ccgccaccgc cgccgtcagc atgcgtgaaa ctcgccgcgg tcaatcacta
      361 cggtggcacc gctgcggacc tgatatgacg ctgtgcggtc actaagccac atcgccaccg
      421 taaggacatc gccgggatac gttggcgcgg agaagcgccc agtgattctc ccgaacctag
      481 ccgggtcgtt gtcacagaca tgccgcaaca gcgcgcgtgc cgcaaagccg taggtgcaca
      541 gcccatgcaa tatcggccgc gcaaaacccc cgcgctgggc gacatctggg tccacgtgga
      601 gtggattgcg gtcacccgct tgccggaaca gcaatgcctg gttaggcatc gtcgccatct
      661 ctatttctag gtctggcgcg cggtcgggga tcgggctggg tgcactgggt ccgctatcac
      721 cgccaaatcc accttctcca cggataaacg atcgcgagac cgcaatcgct atcggttcat
      781 ccgttactgg gtctactgca gtggattcga agttcacgag cgccccgctg cgcttgtccc
      841 atatgtcggt gacacgtcgg gtgacatgaa cggtaccgct ggcggccaat ggcgccaaca
      901 tgaccagtga ctgttcggca tgtaatcgct gggaggcgct gacatctccg aggtcgatct
      961 cgagcccatg ttgttgcatc aacccgatcg cgaaggtagg caagacacgt tgcttgccaa
     1021 gctcagcgca ttcggttgta aattctagat cgaccagcgg atccggcgca ccggcgccga
     1081 cgccgagtgc gtagatttgc gcgtcagctt cggtccagga aacggccgtt ggaccgaacc
     1141 gcttgcccac gatatcgaag gcgagtgcca tttacgcacc agcctcggcc tgtacttcca
     1201 gctcggagac cttggcgcgc aactgttctg cggcacgctg aaggtgcttc tcggtaaccc
     1261 ttcgagcctc ggcaccgtca tgattggcaa gtgcacggac cagtgcgtcg tgctcctcga
     1321 ggctgcaggg gacgtcctcg ggcaacgcat ccagaaagcc gtgcgggatc atccgacccg
     1381 ccatgcccag catggccagg agccgcggcg aaccgcacgc tttgttcact tctcggtgga
     1441 aaagccagtt ctctgtggcg gcgtcgtcac cggaacgcat ggcaatcgct acttccacct
     1501 tcagcgcgcg cacccgcttc agctgctcgg gtgtgatccg ctgcgccgcc cactcggcgg
     1561 ccactccgag tagtgcggcc atcagggcga agttctcgag gatcgcctcc ggagtcgcgc
     1621 ccaacacgat tatccccgtg cgcggggcaa cccggaccag cccgtcgagc gccaattcga
     1681 ggcaggcctc gcgcaccggg gtccggctcg tatcgaattc gacggcgacg gcatccaggt
     1741 cgatccgggc tccaggtgct agcaccccgg tgaggatgcg ttcacgcagc acccggcaga
     1801 ctcgctctcg gatcgttcca ctgcctgcgg ttggcgccgg cactccagcg accggccacg
     1861 gctcgctcat cgcccctcct tcggcatccc tagagtatat cgatggtcat ctgatatatc
     1921 tatatcacgg gggccgaagc gtcgcccctc caacgagaga gctacatgac tcaaactacg
     1981 agaccactca cgacagtcgc tccgctgcgc tccttgctgt tctgcccagc cacttctgcg
     2041 cgtcgtgtcc gcaagatgct cgagctggct cccgacgccg ccgtgcttga cctcgaggat
     2101 gccgttaccg acgacaacaa gaccgcggcc cgccagcagg cacgcgctgg catcgccgaa
     2161 atcgccccga tccgcagcta tgttcgggtc aaccatccgt cgaccgggcg tacacagggt
     2221 gacatcgatg ccgtcgtgca acccgctctc gacggcatcg tgctgccaaa ggtcgaggat
     2281 gtcgacacca tccaagacgc cgacggctgg atcaccgccg cggagcgaca aaacggcgtc
     2341 gagcctggct gcacccggct actggtgctc atcgagtctg cccgaggcat cgccgacgcc
     2401 cgcgagatcc tgcaagccag cgcgcgcatc gagacggccg tcttcggagc acacgactac
     2461 atgctcgatc tgggcatcga tggactcgac cactccgaaa gcgcagagga actcctctac
     2521 gcccgttcgg cactggtcat cgccgcgcgc gccgctcgcg tctcgcagcc gctggacggg
     2581 ccgtttatcg ccttcggcga cacagaccga tttgtcgaaa tgtgccgaca ggcccggcgg
     2641 atgggctttc gcggcaagat gctcatccac cccaaccagg tggctttgag ccaccttggc
     2701 ttcgccccaa ccgacgcgga aatcgcaacg gcaaaccgca tgatcgaaca attcgccgaa
     2761 gccgaggccg aaggtgccgc ggccatcgtc gtcgacggcc gactggtgga ctacccgatc
     2821 gtcgcgcgtg cccgtcgcat catcgaggcg gcagcggagc tgcaatgacg cgatcactcg
     2881 acggcatccg cgtcatcgac gccgcatcac ttgcggccgg tccgctggtc gcgacctacc
     2941 ttggcgaact cggcgccgaa gtcatcaaaa tcgaacagcc cggcaccggc gacgccatcc
     3001 gcagctgggg cgcgcagaaa aacggccatg gactgatgtg gaaaagcgtc gggcgcaaca
     3061 agcgcaccgc gacgctcgac ttgcgcaagc ccgccggcca gcagctattc tgcgagcttg
     3121 cggcggtggc agacgtcgtc atcatcaaca cacgtccctc gacgctcgaa cggtgggggc
     3181 tcagctacga gtcgctcgcc accgtcaatc ccggtctggt catgctgcac gtcaccggtt
     3241 tcggcgcggg cgggcccaac agtgatcgcc ccggattcgg cactctcggc gaagcaatga
     3301 gcggcttcgc ccacaccacc ggcgaacagg atggaccgcc gaccttgccg tcgttcatgc
     3361 tcgctgacgg tgtagccgcg ttgaccggta cctacgcagt tctcgcagcg ctgtaccacc
     3421 gcgacatgca tggtggaaca ggtcagctga tcgacttgaa tctcgtcgag ccgctggcgc
     3481 gcctgcttga acagtctgta ctgacctacg accaactcgg gatcgtgccg acccgcgccg
     3541 gaaacaagtg ggacatcagc gcaccgcgca acacctaccg cactaaggac gaccagtgga
     3601 ttgcggtgtc tggcagcgca ccgaccgtcg ccaaacggat tttcaaggcc atcggccgag
     3661 cagagttggc cgacgatccc gacttcggcg atccccagca gcgactgaag aacgccgatg
     3721 ccgtcgatgc cgcagttgcc gagtggatca tcgcgcgaac cctagacgag gcgatgaccg
     3781 tcttcgaact cgaagaagtt gccgccgcac cggtatacga cgcgcccagg ctgcttgccg
     3841 atcctcatct gcaggcccgc aaaacctacg cgcccgtgga tgatccggat ctgggcagca
     3901 tccggatgca ggcgccggtg ccgcggctga gccagacacc agcggtcatc gaccacacag
     3961 gaagagggct cggcgccgac aacgcctacg tttacggcga aatcctggca ctaacggaag
     4021 aggaaatcga acgactccgg agcgacaacg tcatctgagc ggcgacccgc gtggccgcgc
     4081 agccaccgcg cctttgcaga ggagcgacga ggattcgacc ggatcgacca ccgcactcgt
     4141 aggcgcagta atgttctgcc ctttgatctg acgacagaaa gcgattagtc catggcactc
     4201 ctgctgcgcc cgcggcgatc cgcgctctac ttgcccggca acaacagccg ggcgttggag
     4261 aaggccaaaa cgctgtcggc agacgtgttg atcttcgacc tggaagacgc cgttggcccc
     4321 gacgccaaag ccgactcgcg gacgcgggta atcgaggcga tctcctccgg tagctaccga
     4381 cctcgtgagc ttgtggtgcg cgtcaacggc tcgggcaccg tgtggcacga cgacgatctg
     4441 gcggcggtag ctcgctcagc cgccgacggg
//
LOCUS       DASRJA010000704         7461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4045130,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000704 DASRJA010000000
VERSION     DASRJA010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4045130"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..832
                     /locus_tag="VFC01_31770"
     CDS             56..832
                     /locus_tag="VFC01_31770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN84234.1"
                     /translation="MSTVITGATLTGRVAIVTGASSGIGEATAFRLATLGAKVAVAAR
                     RKDNLDALVARIIAAGGTALALPLDVTDRSAVNAAAQHVADELGPVDLVFNNAGVQLI
                     SPIEDVRFDDWQRQIDLNITGVMNVIGAFVPQLIGSAAQGKAADLITTSSIAATRVLE
                     KFSVYSGTKAYISQLTRLLRVELGRKMVRVSTIEPGMVDTELPLHVTDPDATRLMADL
                     INDIDVLSAADVAETVAFIASVPRHVNLTEITILPTQQAV"
     gene            850..1584
                     /locus_tag="VFC01_31775"
     CDS             850..1584
                     /locus_tag="VFC01_31775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010843884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN84235.1"
                     /translation="MSTNPKTVLITGASSGIGEAAALRLADDGHRLFLGARRTERLEA
                     LVERIETAGGTASLRRLDVTDAADVQQFVTDAADTYGGVDVMINNAGVMPLSALAENK
                     VDEWNRMIDVNIRGVLHGISAALPVMRSQGSGHIVNVASIGAHEVEPTAAVYCATKFA
                     VWAISEGLRKEQAGDIRVTVISPGVTESELAESISDEQARAAMREYRALAIPASAIAD
                     AIAFAIGQPPEVDVNEIIVRPAASTH"
     gene            1903..3333
                     /locus_tag="VFC01_31780"
     CDS             1903..3333
                     /locus_tag="VFC01_31780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016880897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN84236.1"
                     /translation="MEMLLAGEWRDAGDRATETVLSPYDGRPVGSVPLATIDDAAAAV
                     DYAEIAARVQRETPAHERVAVLLRAADLADQRAEDIAQTISAETGKPISEARGEASRS
                     GAIIRLAAHEGSQLYGSTLPLDANPGTGLEKIGFTVRQPCGIVVAITPFNYPALLVLH
                     KIAPALAAGNAVVLKPARSTPLTALKLAQCFVDAGLAPGALSVLTGAGGTLGDRLVTD
                     PRVRKVSFTGSTATGTHIAAVAGIKKLSLELGASCPVIVLPDADIEMAASAVAAGGYI
                     NAGQVCISVQRVIVDERVEAEFLDALVPKVDAITLGDPAQEDTRLGSLISETEATRVA
                     SAIQTAGDSGAKVLTGGDREGAVITPAVVAAVDPQSPFSRDELFGPAVAVSSAPDIAA
                     AIAIANDNEYGLGAGIFTSDVTATVRAMRQIDAGNIHINWTPLWRADLMPYGGLKGSG
                     IGKEGVRSAVTEMTEEKTIVIHGRPW"
     gene            3353..5308
                     /gene="iolD"
                     /locus_tag="VFC01_31785"
     CDS             3353..5308
                     /gene="iolD"
                     /locus_tag="VFC01_31785"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012687763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HZN84237.1"
                     /translation="MISTSHKSVRLTAAQAIVKYLAAQYSLADGARRRLIPAALGIFG
                     HGNVAGLGQALDELSDELPFIQGRHEQYVAHIATAYAKATRRHATLAVTASIGPGALN
                     LVTAAGLATVNRLPLLLLPGDTYATRHQGPVLQQLEHASQADATVNDAFRPVSRFFDR
                     ITRPEQLLTALPQAMRVLTSPTDTGAVVISLPQDIQSHAYDFPVEFFEPRDWKIRRPL
                     PDPDEITQVAALIREANRPVLIAGGGIHYSDGHAALEQFAETAGIPVVETFGGKGAVT
                     RDAWWQVGGVGLEGNFAANSLVKQSDLIISVGTRLTDFTTGSQSIFENDSVQFASLNV
                     VDADTRKQGAVGILADARLGLQALTQALRDHTTSAAWRDTVVAAKADWAPLRAAALDA
                     DIAVRREEHPDAPVTDAVLTQAQLIGLLQEHAQSGDTIIAAAGGPPGDLQKVWDATGN
                     RRCHLEFGFSCMGYELPAAMGVRLAHGDDGSRIVTFIGDGTFLMAPTELVTAAQERLD
                     ITIVVSENHGYQVIHRLQMNRSGREFGNEFRYRTDGDIIDGTAERKAPRLEGDYLDVD
                     LRKIAEGLGATAIRATTAVEVRDALADTRDTRGPVVLVVPTVPHVDLPGGDVWWDVAP
                     AEVSGQEWVQALRAEYEQARGAQRWFG"
     gene            5305..6087
                     /locus_tag="VFC01_31790"
     CDS             5305..6087
                     /locus_tag="VFC01_31790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005243490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZN84238.1"
                     /translation="MSATPLDGGALRRRLANGEITVGTFVGMASATATEVCAAAGADW
                     VLIDLEHGSGSEDVISDSVLAAASYGVPAIVRVESGERIRIGRVLDQGATGVMVPRLD
                     KPDEVATVLSHLRYPPHGDRGVATYNRSCRWGLDRSPLTDPTQNTLGVIQIETLAALD
                     AVDEIAALDGVDVLFIGPLDLSFALGVPLRFDDPEFVAATGRVLDAAAAHRKTAGILA
                     ANAAMATRYVERGFRFVAIGSDSTMLAAALSDAFTAARTTHT"
     gene            6111..7331
                     /locus_tag="VFC01_31795"
     CDS             6111..7331
                     /locus_tag="VFC01_31795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009473183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN84239.1"
                     /translation="MSTTNRRIGVGLISVGWMGQLHSRAYRSVPIVYPELGINPELVI
                     AADSAPDRAEFARDVLGYTRATTDYREVLADAEVDVVSICAPNFLHAEIGIAAAEAGK
                     HFWIEKPVGRSYAETSAIASAVTKAGVITSIGYNYRHAPAVEHARSLIAAGKLGWITN
                     VRAAFFSGYANEPKGALSWRFNRELAGSGVLADLFSHASDLCQYVVGPIAEITALTST
                     VYTERPKLAMGSGTHFDVIEGGEMGTVENEDYAAALVRFGADAQGSGAVGTIEASRVI
                     VGPACGYQIEVYGTDGSVKWDFERMNELQVRIGRAADEPGYITVNANPKHGDYSRFQP
                     GPGNAMGFDDLKTIEAKKFLLAVAGGVAANSNVHDAVSAAAIVTAAEESAATGQWQKI
                     EPVAGTTASRITAG"
BASE COUNT         1299 a         2599 c         2406 g         1157 t
ORIGIN      
        1 tcggcgctgt tgtctcggat gcacacaccc aaacactctc gaagggacga accacatgtc
       61 cacagtcatc acgggggcca cgctgacggg gcgcgtcgcc atcgtcaccg gggcctcgag
      121 tggcatcggg gaggccacgg ctttccggtt ggcgaccctc ggcgccaagg tcgccgtcgc
      181 ggcgcgtcgc aaggacaacc tcgatgctct agtggcgcgc atcatcgccg ccggcggcac
      241 cgcactcgcc ctgcccctcg atgtcaccga ccgatcagcc gtcaacgctg ccgctcagca
      301 cgttgccgac gagctcggcc cggtcgatct ggtattcaat aacgctggcg ttcaactgat
      361 ctcaccgatc gaagacgtca ggttcgacga ctggcagcgc cagatcgacc tcaacatcac
      421 cggcgtgatg aacgtcatcg gcgcgttcgt cccgcaactc atcgggtccg ccgcgcaggg
      481 taaagccgcc gacctcatca ccacctcctc gatcgcggcc acccgggtgc tggagaagtt
      541 ctccgtgtat tccggaacca aggcctacat cagccagctg accaggctgc tgcgcgtcga
      601 actcggccga aagatggtgc gcgtctcgac gatcgaaccg gggatggtgg ataccgaact
      661 gccgcttcat gtcaccgacc ccgacgccac ccggttgatg gccgacctca tcaacgacat
      721 cgacgtgctc agcgcagccg acgtagccga gaccgtcgcc ttcatcgcct cggtacctcg
      781 ccacgtcaac ctcaccgaaa tcaccatcct gcccacccag caagccgtct agcgaaaggg
      841 aacaccacca tgagcacgaa cccgaagacc gtcctgataa ccggcgccag cagtggaatc
      901 ggcgaagccg ccgcactgcg gctcgccgac gacggccacc ggctcttcct cggcgcccga
      961 cgcaccgaac ggctcgaagc cttggtggag cgaatcgaaa ccgccggcgg gactgcgtcg
     1021 cttcgacgtc tggatgtcac cgacgccgct gacgtccaac aattcgtcac cgatgccgcc
     1081 gatacctacg gcggggtgga cgtcatgatc aacaacgcag gagtcatgcc gctgtccgcc
     1141 ctggcggaga acaaggtcga cgaatggaac cggatgatcg acgtcaacat ccgcggtgtg
     1201 ctccacggga tcagcgccgc ccttcccgtg atgcggtccc aaggaagcgg acacatcgtc
     1261 aacgtggcct ccatcggcgc ccacgaggtg gaaccgaccg cggccgtcta ctgcgccacc
     1321 aagttcgcgg tctgggccat ttcggaaggg ctgcgcaagg agcaagccgg cgatatcagg
     1381 gtcaccgtca tttcgccggg agtcaccgaa tccgaactcg ccgaatcgat ctcagatgaa
     1441 caagcccgcg ccgcaatgcg ggaataccgg gcgcttgcca ttcctgcatc ggcgatcgcc
     1501 gatgccatcg cgttcgccat tgggcaaccc cccgaggtgg acgtcaacga gatcatcgtg
     1561 cggcccgccg ccagtaccca ctagcccgca gcggctgctg ccctgcaggg ctttcacgaa
     1621 ccaccgatat tcatcaccca ggagcctgaa caaagtcacc tcgcttgatg ggaaattgac
     1681 gcgattctcc cattgattga ggtctagatc acagcaacac tctatgtgtg ccgcctcaac
     1741 gccgaccggc ttgccggcca tgaggcagtg caggtggtcg ccgaccggtg atcgatgcga
     1801 cgttgtgcgc gtcgtcttcg aggcgacgat gtagcgattc ggttgcggcg ctggcgattc
     1861 gataccaccg acgcatttgt gttttgaaca aggaggcttg tgatggagat gctgcttgcc
     1921 ggcgaatggc gcgacgcggg tgaccgtgca accgagacgg tgctgtcccc gtacgacggc
     1981 cgaccggtcg gttcggtgcc gctggccacc atcgacgacg ccgcggcggc cgtcgactac
     2041 gccgagatcg cggcacgggt gcagcgggag accccggcac atgagagggt ggcggtgctg
     2101 ctgcgggcgg ccgatctggc cgatcagcgc gccgaggaca tcgcgcagac gatcagcgcg
     2161 gagaccggca agccgatcag cgaggcgcgc ggtgaggcaa gtcgctccgg tgcgatcatc
     2221 cggctggccg cgcacgaggg cagccaactc tacggctcga cgctgccgct ggacgcgaac
     2281 cccggaaccg gtttggagaa aatcggtttc acggtgcgtc agccgtgcgg aatcgtcgtg
     2341 gcgatcaccc cgttcaacta cccggcgctg ctggtgctgc acaagatcgc cccggcactg
     2401 gccgcgggta acgcggtcgt gctcaaaccg gcccgctcga caccattgac cgcgctcaag
     2461 ctcgcgcagt gcttcgtcga cgccggcctt gcgccgggtg cgctctcggt actcaccgga
     2521 gccggcggca ccctgggcga ccggctggtc accgacccgc gggtacgcaa ggtctcgttc
     2581 accggctcga cagccaccgg gacacacatt gccgcggtcg ccgggatcaa gaaactgtct
     2641 ctggagctgg gcgcctcatg cccggtgatc gtgctgcccg acgccgacat cgagatggcc
     2701 gcatccgcgg tggccgccgg gggttacatc aacgccggcc aggtgtgcat ctcggtgcag
     2761 cgggtgatcg tcgacgaacg cgtggaagcc gagttcctcg acgcgctggt gccgaaggtg
     2821 gacgccatca ccctgggcga ccccgcccag gaggacaccc ggctggggtc gctgatctcc
     2881 gaaaccgagg ccacccgagt ggcctcggcg atccagaccg ccggcgattc cggcgcgaag
     2941 gtcctgacgg gcggcgaccg cgagggtgct gttatcaccc ccgccgtggt ggcggccgtc
     3001 gacccgcagt caccgttctc ccgcgacgaa ttgttcggac ccgcggtcgc ggtgtcgtcg
     3061 gcccccgaca tcgccgccgc gatcgcgatc gccaatgaca acgagtacgg cctgggcgca
     3121 ggcattttca cctccgatgt gacagcgacg gtacgcgcga tgcgccagat cgacgccggc
     3181 aacattcaca tcaactggac cccgctgtgg cgggccgacc tgatgcccta tggcgggctg
     3241 aagggcagcg gcatcggcaa ggagggtgtc cgctcggctg tcaccgaaat gaccgaagaa
     3301 aagaccatcg tcatccacgg ccgcccgtgg tgaaccccga aggaaaccag ccatgatcag
     3361 cacctctcac aagtcggtcc ggctcaccgc cgcccaggcc atcgtcaaat acctcgccgc
     3421 ccagtacagc ctcgccgatg gtgcccggcg ccgcctaatc cccgccgcgc tgggcatctt
     3481 cgggcacgga aacgtcgccg ggttgggtca ggccctcgat gagctgtccg acgaattgcc
     3541 gttcatccag ggccgccacg agcagtacgt cgcgcacatc gccaccgcgt acgcgaaggc
     3601 cacccgtcgg cacgccaccc tcgcggtcac cgcgtccatc ggcccgggcg ccctgaacct
     3661 ggtcaccgcc gccggtctgg cgacggtcaa ccggctgccg ctgctgttgt tgccaggaga
     3721 cacctacgcc accaggcacc agggccccgt gctgcagcaa ctcgaacacg ccagccaggc
     3781 cgacgccacc gtcaacgacg cattccggcc ggtgtcgcgg ttcttcgacc ggatcacccg
     3841 cccggagcaa ttgctcaccg cgctgccgca ggcgatgcgg gtgctcacca gccccaccga
     3901 caccggcgcg gtagtgatct cgctgccgca ggacatccaa tcgcacgcct acgacttccc
     3961 cgtcgagttc ttcgaacccc gggactggaa gattcgccgc ccgttgcccg accccgacga
     4021 gatcacccag gtcgccgcgc ttatccgcga ggcgaaccgg ccggtgctga tagccggcgg
     4081 tggcatccac tactccgacg gccacgccgc gctggagcag ttcgccgaga ccgcgggcat
     4141 cccggtggtg gaaaccttcg gcggcaaggg cgcggtcacc cgcgacgcat ggtggcaggt
     4201 cggtggcgtc ggccttgaag gcaacttcgc cgccaattcc cttgtcaaac aatcggattt
     4261 gatcatcagc gtgggcaccc gactgaccga cttcaccacc ggctcgcagt cgatcttcga
     4321 aaacgactcg gtgcagttcg cgtcgctgaa cgtcgtcgac gccgacaccc gcaagcaggg
     4381 cgcggtcgga atcctcgccg acgcccgcct cgggctgcag gcgctcaccc aggcgctgcg
     4441 cgaccacacc accagcgcgg cgtggcgcga cacggtcgtc gcggcgaagg ccgattgggc
     4501 gccgctccgc gctgccgcgc tcgacgcgga catcgccgtt cggcgtgagg agcaccccga
     4561 cgcccccgtg accgacgccg tgctgaccca ggcgcagctg atcgggttgt tgcaggaaca
     4621 cgcccaaagt ggcgacacca tcatcgccgc cgccggcggg ccccccggcg acctgcagaa
     4681 ggtgtgggac gccaccggca accggcgctg ccacctcgaa ttcgggttct cctgcatggg
     4741 ttacgaattg cccgccgcca tgggcgtccg cctggcccac ggcgacgacg ggtcgcggat
     4801 tgtcacgttc atcggcgacg gcaccttcct gatggcgccg accgaactgg tcaccgctgc
     4861 gcaggaacgc ctcgacatca cgatcgtggt gtcagagaat cacggctacc aggtgatcca
     4921 ccgtctacag atgaaccgca gcggacgtga gttcggcaac gagttccgtt accgcaccga
     4981 cggcgacatc attgacggga ccgccgagcg caaggctccc cgccttgaag gtgactacct
     5041 ggacgtggat ctgcgcaaga tcgcagaagg cctcggtgcg accgccatcc gtgccaccac
     5101 cgcggtcgag gtgcgcgatg cattggccga cactcgcgac acccgcggcc cagtggtcct
     5161 ggtggtgccg acggtgccgc acgtcgacct accgggcggg gatgtgtggt gggacgtggc
     5221 ccccgcggag gtgtccgggc aggaatgggt gcaggcgctg cgcgccgagt acgagcaggc
     5281 ccgcggcgcg cagcggtggt tcggatgagc gccactccct tggacggtgg cgcgctgcgg
     5341 cgccggttgg ccaacggcga gatcacggtc ggcacattcg ttggcatggc gtcggcgacg
     5401 gccaccgagg tctgcgccgc cgccggggcc gactgggtgc tcatcgacct cgaacacggt
     5461 tccgggtccg aggacgtgat ctctgacagc gtgctcgccg cggcgagtta cggggtgccg
     5521 gcgatcgtgc gggtggagtc gggtgagcgg atccggatcg gccgcgtgct cgaccagggc
     5581 gcgaccgggg tgatggtgcc gcggctggac aagcccgacg aggtggcgac cgttctttcg
     5641 cacctgcggt acccgccgca cggtgatcgg ggcgtggcca cctacaaccg gtcctgccga
     5701 tggggattgg accgttcgcc gctgaccgat cccacccaga acacgctcgg cgtgatccag
     5761 atcgagacgc tcgctgcgtt ggacgcggtc gacgagatcg ccgccctgga cggtgtggac
     5821 gtgttgttca tcggtccgct ggatctgtcc ttcgcgctcg gtgtgccgct gcggttcgat
     5881 gaccccgaat tcgtcgccgc caccgggcgg gtgctcgacg ccgcggccgc gcaccgcaag
     5941 accgccggga ttctcgccgc gaacgccgcc atggcgacac gttacgtcga gcgcggattc
     6001 cgtttcgtgg caatcggttc cgactcaacc atgctggccg ccgcgctgag cgatgcgttc
     6061 accgcggccc gcaccaccca cacctgacaa ccagaaaggc agaacaccaa gtgagcacaa
     6121 ccaaccgccg catcggcgtc gggctgatca gtgtcggctg gatggggcag ctgcattccc
     6181 gcgcctaccg cagcgtgccg atcgtctacc ccgagctcgg catcaacccc gagctggtga
     6241 tcgccgccga cagcgccccg gaccgggctg agttcgcccg cgacgttctc ggctacaccc
     6301 gtgcgacgac cgactaccgc gaggtgctgg ccgacgccga ggtggacgtg gtgtcgatct
     6361 gtgcccccaa cttcctgcac gccgaaatcg gcatcgcggc agccgaagcc gggaaacact
     6421 tctggatcga aaagccagtg gggcgcagct acgcggagac ctcggccatc gcgtcggccg
     6481 tcacgaaggc gggtgttatc acctcgatcg gctacaacta tcgccacgcc cccgcggtgg
     6541 aacacgcccg cagcctgatc gcggccggca agctgggctg gatcaccaac gtccgcgccg
     6601 ccttcttctc cggatacgcc aacgagccca agggtgcgct gtcgtggcgg ttcaatcggg
     6661 aactcgccgg ctcgggtgtg ctggccgatc tgttcagcca tgccagcgac ctgtgccagt
     6721 acgtcgtcgg cccgatcgcc gagatcaccg cgctgaccag caccgtctac accgaacgtc
     6781 cgaagctcgc aatgggttcg gggacacatt tcgatgtgat cgagggcggt gagatgggca
     6841 ccgtcgagaa cgaggactac gccgcggcgc tggtccggtt cggcgccgac gcccagggat
     6901 cgggtgcggt cggcaccatc gaggcatccc gggtgatcgt gggtcccgca tgtggctacc
     6961 agatcgaggt gtatggcacc gacgggtcgg ttaagtggga cttcgagcgg atgaacgagc
     7021 tacaggtgcg catcgggcgt gccgccgacg agcccggcta catcaccgtc aacgccaacc
     7081 ccaaacatgg tgactactcg cgctttcaac ccggaccggg caacgcgatg ggcttcgacg
     7141 acctcaagac cattgaagcc aagaagttcc tgctggccgt cgcgggcggt gtggccgcga
     7201 attccaatgt gcacgacgcg gtctcggctg cggcgatcgt caccgccgcc gaagagtccg
     7261 ccgcgaccgg gcagtggcag aagatcgaac ccgttgccgg caccacggca tcccgcatca
     7321 cggccgggtg aacgatgtcg accgcttctc acgaacctgc cgtgacgaca tcggtcccga
     7381 tccagacgtt cgtcgcgttg ggcgcgatcg accccgagct ggtggagccg tttctggcgc
     7441 cccatcaacg gcttgtgctg a
//
LOCUS       DASRJA010000705         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1870315,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000705 DASRJA010000000
VERSION     DASRJA010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870315"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2198
                     /locus_tag="VFC01_31800"
     CDS             <1..2198
                     /locus_tag="VFC01_31800"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HZN84240.1"
                     /translation="HEALRRRLASVISGLGSSLPFGQWPDHPLIRYKEPAANDARLRE
                     LLGLDPADTTPRPQLLNLLKLEAPLAVQTRTEPGLFPINKFSSVPLLIRVVRFDAGAS
                     AGYDEFRRIMVVPNIQVLDLVTTPPGPSVRVTGVDVASTVNGGFIPLAPDGIVVIAAG
                     TIESTRLAKLTFQASLAGAAGDRIGTNLMAHLRSNITLRLPMASLGLTAADLADKPVP
                     VSALFAKGRIAVGNRNRYFHLQITASAGPGGGDNSESLLFKKVPDIDFLQTMKAATAT
                     HVVVTLRGIGEMMPGNPSNTVGLALFDSDGNRPAAWVTVGNVRAFVNDPAAPPATYQT
                     TPLSNETKQDADIWEAMDSWSDELAVALAGGQEFEILTPSGAKKLPTTATAADVKAAF
                     PHRAPRRDTLGSTHHEAGTLRMGNAADAVSDDLGRLHGTPNCYFAGPCLFPTIGSPNP
                     MLTGTAVARRTADYLAGKLPPARAEAPVLAQPPTVPTDGPGWETLFDGSDASFRRWRR
                     AGGNAGGKDRFGIDRPACDLRLVAGQIATLGNGDHALLYYQPKAFANFILRVQFHIVD
                     GSFHNGGVFVRVRRPDLDLPAVLAQRADALGRPWRTNRAWTAVFSGFEVQIDDNAKTQ
                     GLRRNRTGAIYDVPAGDPGEPSDQMYSPGPSLQPGVWFEFDITVQNNHYEVRLGRADG
                     SPKVTVSTFDNMDLNRGLSSAADPDGGFIGLQAHNDGRVYYRHIRIKTL"
BASE COUNT          428 a          769 c          783 g          399 t
ORIGIN      
        1 tgcacgaggc cctccgccgc aggctggcgt cggtcatcag cggtctcggc agctcgctgc
       61 cattcgggca gtggcccgac catccactga tccggtacaa agaacctgcc gcgaacgacg
      121 cccggctgcg tgagcttctc ggactcgacc cggccgacac cacgccgcgg ccgcagctgc
      181 tgaatttgct gaagctcgaa gccccgctgg cggtgcagac gcggacggaa ccgggtctat
      241 tcccgatcaa caagttcagc tccgttcccc tgttgatccg cgtagtccgg ttcgacgcgg
      301 gcgcctcggc ggggtatgac gagttccggc gcatcatggt cgttccaaat atccaggtgt
      361 tggacctagt gaccacgccg cccggcccct cggttcgggt gacaggtgtg gacgtggcgt
      421 cgacggtcaa tggaggcttt atcccgctcg ccccggacgg gatcgtggtc atcgcggcgg
      481 ggacaatcga gtccacccgc ctcgcgaagc tgacatttca agcgtcgctg gcgggggcgg
      541 caggtgaccg aatcggtacg aatctgatgg ctcatctgcg atcgaacatc acgctccgac
      601 tacccatggc cagcctcggt ctcactgcag cggatctcgc agataagccg gtgccggtgt
      661 cggcgctgtt cgcgaagggc cgcatcgccg tcggcaaccg caaccgctac tttcatctgc
      721 agatcacggc gtcggcgggg ccgggagggg gcgacaattc cgaatcactc ctgttcaaga
      781 aggtgcccga catcgacttt ctgcagacca tgaaggcggc gacggccact cacgtggtgg
      841 tcacgctgcg cggcatcggc gagatgatgc cgggcaaccc ctcgaacaca gtggggcttg
      901 cgcttttcga cagcgatggc aatcggcccg ccgcttgggt gacggtcgga aacgtcaggg
      961 cgttcgtgaa cgacccggca gcaccgccgg cgacttacca gaccacgccg ctcagcaacg
     1021 agacgaagca ggacgccgac atctgggagg cgatggacag ctggagcgat gagctcgccg
     1081 tcgcgctggc aggaggccag gagttcgaga tcctgacccc gtccggcgcc aagaagctgc
     1141 cgaccaccgc gacggcggcc gacgtgaagg ccgctttccc ccaccgcgcg ccccgtcgcg
     1201 atacgctcgg ctccacgcat cacgaggccg gaacgttgag aatgggcaac gccgccgatg
     1261 cggtgagcga cgatctgggc cggttgcacg gcacgccgaa ctgctacttc gcggggccgt
     1321 gcctgttccc taccatcggt tcacccaacc ccatgttgac gggaacggcc gtcgcgcggc
     1381 ggaccgccga ctatctggca ggaaagctac cgcctgcacg ggcagaagct ccagttctgg
     1441 cgcaaccacc gaccgttcca accgatggcc ctggctggga aacgttgttc gatgggagcg
     1501 acgcctcgtt ccgccggtgg cgccgcgcgg gtggcaatgc agggggaaag gatcgcttcg
     1561 gcatcgatcg tcccgcgtgt gacttgcgac tggtggcggg ccagattgcg acgctgggaa
     1621 acggcgatca tgctctgctc tactaccagc ctaaggcgtt cgcgaacttc atcctgcgcg
     1681 tgcagttcca catcgtcgac ggcagctttc acaatggcgg tgtgttcgtc cgagttcgcc
     1741 ggcccgatct cgacctgccc gctgtgctcg cgcaacgtgc ggacgctctc ggacggccgt
     1801 ggcgtacgaa ccgggcgtgg accgcggtct tcagcggctt cgaggtgcag atcgacgaca
     1861 acgcgaaaac acagggcctc aggcgaaacc gcaccggggc gatctatgac gtcccagctg
     1921 gtgatccggg cgaaccctcc gaccagatgt attctcccgg accgtctttg cagccgggcg
     1981 tatggttcga attcgacatc accgttcaga acaaccacta tgaggtaagg ctggggcgtg
     2041 cggatggctc tccaaaagtc accgtctcga cgttcgacaa catggacttg aaccgggggc
     2101 tttcaagcgc ggcggatccc gacggcgggt tcatcggcct acaagcgcac aacgatggcc
     2161 gggtttacta ccggcacatt cggatcaaga ctctgtagac gcggtccaaa aataggcgag
     2221 ccgggcaccg cctgctagag gacggcggcc gacagccgag cggcagtatt cgagcgagcc
     2281 ttgctgagcg cggggcggcg gtgtcgggtt gagtggtgat cttgaagcgg cgtccgcgga
     2341 tggccccgca gagcccgagc tggcgcatca ggcgctgca
//
LOCUS       DASRJA010000706        12187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3564443,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000706 DASRJA010000000
VERSION     DASRJA010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12187
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3564443"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VFC01_31805"
     CDS             <1..814
                     /locus_tag="VFC01_31805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZN84241.1"
                     /translation="AAYAGHVDEARRAIEAALAASQRCAANLLVVWTITTLGFLEVSL
                     GNYDAALTAVAPLLSSLDAAPDGTEIPFASFVPDAVEAMIALGRIADPEPLVSALERN
                     GARLDRAWMLAIGARCRAMLLAANDDVGAAYRAAEAAMAHHDRLPMPFERARTQLLVG
                     QLQHRQKNRQAATATLREALTTFEELGTPLWAERARAELKRACGIRTRAELTASEQRV
                     AELAATGITNREMAAALYISPKTVEANLSRIYRKLNIRSRAELGRIIGKTEQ"
     gene            861..1139
                     /locus_tag="VFC01_31810"
     CDS             861..1139
                     /locus_tag="VFC01_31810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84242.1"
                     /translation="MRPHYLVEWYRSAIADEPLDHSVARLIQCAASMSAAGSPVNLLA
                     TLAVPTDEIVLAVFDSASELSVVEVCRRAGCPAQRLTAAVDARFWPSA"
     gene            1185..2123
                     /locus_tag="VFC01_31815"
     CDS             1185..2123
                     /locus_tag="VFC01_31815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN84243.1"
                     /translation="MGNSVDTRPRSVREQAKRHAHEVRTHMEERRQQQDDGLTGWLNK
                     RAGDWELSGPDEATMQRQKYFWNFLVDHWFRMEFDGWENLPDPPVLLVGIHSGAPFVW
                     DAWTVGVQWWRRFGQDRPLHGTAHDVLMAMPLVGRYFRAMGVLPAAPDSMATALAEGR
                     DVAVWPGGEVDSLRPWTERDRANLAGRKGFVKMAIRAGVPIVPIATVGGADAMPVLIR
                     GDQLSRSLRLDKLLRLKVFPIAASLPWGIAPAALPQFPLPAKIRTRLMPAVELDHDPK
                     RADDDEYLEEKYWEVQDSIQQGMDALARKRALPLFG"
     gene            2366..2869
                     /locus_tag="VFC01_31820"
     CDS             2366..2869
                     /locus_tag="VFC01_31820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84244.1"
                     /translation="MIAVLIVVAVVLGVGVLGVYFVYRTVSNSVSDAVGGGELNCPSA
                     EDISTLVGSEVNGPTGGNIVVASGCYYLPKGPDDTIEVIIVSGSKLIADDEIRSFESE
                     GRAANVDVRSIDVGDRGHAWASSTKSAAIAVGEQSLVSVEVQGKDFTAIPDKTDAAIA
                     ILERVLH"
     gene            complement(2943..3743)
                     /locus_tag="VFC01_31825"
     CDS             complement(2943..3743)
                     /locus_tag="VFC01_31825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZN84245.1"
                     /translation="MRLATWNVNSIRARVDRVTDWLERADVDVLAMQETKCSDEQFPA
                     MPFLAAGYEVVHCGFNQWNGVAIASRVGFDDVQVGFDGQPVWSDKPDVEAAAEARALG
                     ATCNGVQVWSLYIPNGRFVGSPHYYYKLEWLAALRATAQAWLTDDPSAQIALVGDWNI
                     APTDEDVWSPEFYANSTHVTPPERAAFQAIVDTQFADVVRPFTPGPAVYTYWDYTQLR
                     FPKNRGMRIDFILGSPALADRVTHAEIVREERKGKAPSDHAPVLVELT"
     gene            complement(3747..4628)
                     /locus_tag="VFC01_31830"
     CDS             complement(3747..4628)
                     /locus_tag="VFC01_31830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZN84246.1"
                     /translation="MPEVGTRVVVRYRLPAGSAPPLTDVVGQLLDVGPTLRVRTKRGD
                     VVAIAADDVVAIKALAAAPVRTGDIRNLEHAAALAWPGAEHQWLGGWLLRFGHGRTRR
                     ANSAVPLNVSTSDDVSEIIDWYAAHGVAPLVAAPDRLFRIPPTVSTGAENLVMTGDVA
                     VQGSSRVTIATAPDDDWLRLYEREVPVDVLTAVIDGEVAFATVGDASVGRAAVTTAPD
                     GTRWVGLSAVHVVEAARRRGLARELCGALLGWGGDRGATRAYVQVLADNTAATQLYES
                     MGFTVHHRSRYLDGRSL"
     gene            complement(4628..5221)
                     /locus_tag="VFC01_31835"
     CDS             complement(4628..5221)
                     /locus_tag="VFC01_31835"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZN84247.1"
                     /translation="MAVVPIRIVGDPVLHTATEPVTVNADGSLPDDVVQLIKDLYETM
                     DAANGVGLAANQIGVSKRVFVYDCADERGRTTRRRGVVVNPVLETTEVPETMPDPEND
                     DEGCLSVPGESFPTGRAKWARVTGLDADGTPITIEGTDLFARMLQHETGHLDGFLYLD
                     RLVGRHARSAKRSVKSHGWGVPGLTWMPGKDPDPFGH"
     gene            5358..5666
                     /locus_tag="VFC01_31840"
     CDS             5358..5666
                     /locus_tag="VFC01_31840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HZN84248.1"
                     /translation="MDGAIARTEQSGDDSDLTDGLTRREHDILAFERQWWKYAGSKED
                     AIKELFSMSATRYYQVLNALVDRPEALAADPMLVKRLRRLRASRQKARAARRLGFDVT
                     "
     gene            5709..6176
                     /locus_tag="VFC01_31845"
     CDS             5709..6176
                     /locus_tag="VFC01_31845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HZN84249.1"
                     /translation="MNQRNSSGLPLRAMVMVLLFLGVVFLLVGFQAMGSGDSEGDQSS
                     SIATSTITTTSSATTSPSPAPARADVRVYNISSTEGAAETTANRLREAGWNVTETGNL
                     SLPEVTATTVFFSDAPGEHEAADEVGKLLQAPVQPRLPELTEQPPGVVVAVTG"
     gene            6187..6894
                     /locus_tag="VFC01_31850"
     CDS             6187..6894
                     /locus_tag="VFC01_31850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HZN84250.1"
                     /translation="MLKTVAAAAFFAVPVVALSACSPNEPIATSPGTTPSVWTGSPSP
                     SASPGEAEGGHGSEGQAASGEKLTAQLKLADGTAVATADIEFSGGFATVTVETTTPGK
                     LTPGFHGLHIHAVGKCEASSVAPTGGAPGDFNSAGAHFQKSGHTGHPASGDLSSLQVR
                     EDGTAKLVTTTDAFTAEDLLGGAKTAIIIHEKADNFANIPPERYQQANGAPPPDETTL
                     ATGDAGKRVACGVIGTG"
     gene            6875..8008
                     /locus_tag="VFC01_31855"
     CDS             6875..8008
                     /locus_tag="VFC01_31855"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HZN84251.1"
                     /translation="MLSAPASSHIDFAGSPRPTLGVEWEFALVDAETRDVSNEAAEVI
                     VELGENNPHVHKELLRNTVEIVTGICDSVPQAMEDLRSTLVTARKIVRDRGMELFCAG
                     THPFAKWSPGSLTDAPRYAELIKRTQWWGRQMLIWGVHVHVGISSAHKVMAINTSLLN
                     HYPHLLALSASSPFWDGEDTGYASNRAMMFQQLPTAGLPFHFQTWAEWEGFVRDQKKT
                     GIIDHMNEIRWDIRPSPHKGTVEVRIFDGVSNIRELSALVALTHCLIVDLDHRLDAGE
                     RLPTMPPWHVQENKWRAARYGLDAVIILDEDSNERLVTDDLDELLNKLEPVAKSLNCA
                     DELARVSDIYRLGGSYQRQRRVAEETDGDLRAVVDALVGELEL"
     gene            8011..8643
                     /locus_tag="VFC01_31860"
     CDS             8011..8643
                     /locus_tag="VFC01_31860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZN84252.1"
                     /translation="MPITPMFPLEVAMLPGEELPLRIFEPRYTALVQDCLAADDPAFG
                     VVLISAGREVGGGDTRSDVGALAHITECADFGDGRYRLRCVMAERIRVLEWHPDDPYP
                     RAMVEVWPDEPGPAVDVEAIRDVEDRMAALFERIARVRGAEVNPRDIVAGADASGDAA
                     MWLYALASRVPMGQADRYAVLSAPTVAARTAALRDAVETVTAMVEFELSE"
     gene            complement(8644..9486)
                     /locus_tag="VFC01_31865"
     CDS             complement(8644..9486)
                     /locus_tag="VFC01_31865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN84253.1"
                     /translation="MDRDTYREQSLHTWGQMAPGWEHRREWLMQMTAPVSDWLVGKLD
                     PHGGQTILEIAAGTGDLGFRVAEHVGQLGRVISTDFAREMIDVARRQGRARGLANVEY
                     RVLDAESMDIDDDTVDGVVCRFGYMLMADPAVALMETRRVLRSGGPLAFAVWRTADAN
                     PWAAVPGMTLVQRGHMPAPEPGAPGIFALAEPARISELVTETGFGEPELEEVALDFPF
                     QDFDDLWDFLVHLAGPLAQVIIALPEDERSATRAAIEANAEPYRNEDDSYTFPAAAWC
                     VLTR"
     gene            complement(9605..10513)
                     /locus_tag="VFC01_31870"
     CDS             complement(9605..10513)
                     /locus_tag="VFC01_31870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZN84254.1"
                     /translation="MTRTDDDSWDITEGVGQTALGVAWSRSQESTSECPLFIDPYAQL
                     FLDAAVERGWQQPPSYMLERIRSISAYAASRTKWFDEFFIAAGAHGIGQAVILAAGLD
                     ARGWRLPWVDDTVLYEIDQPKVLEFKAQTLAQHDATPSVSRYVAVPVDLRHDWPKALR
                     DAGFDTTVPTAWAAEGLLPYLPAAGQDLLFERIGDLSAEGSRIGVESFGRGFFDRDYL
                     ASRREQLRRLREEAGEDPDANVPDTQDLWYVEDRTDVAEWLTEHNWEVTAVEAVELMT
                     RYGRCSPDQDDAVAPRTVFVEGHLVC"
     gene            complement(10500..10862)
                     /gene="mnhG"
                     /locus_tag="VFC01_31875"
     CDS             complement(10500..10862)
                     /gene="mnhG"
                     /locus_tag="VFC01_31875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HZN84255.1"
                     /translation="MSALDIVTAVLVLGGSTLALTAAIGVVRFPDTLTRMHAATKPQV
                     LGLLLVLVGAAIRLRGHADVGMVILTGLFTVITAPVVANRVGQLAYREQNIRDDLLTK
                     DEMLEFAENRESGGDDQN"
     gene            complement(10859..11146)
                     /locus_tag="VFC01_31880"
     CDS             complement(10859..11146)
                     /locus_tag="VFC01_31880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HZN84256.1"
                     /translation="MTIVWVIAAVMLTAAAFITMFRLLAGPSTLDRLVALDTLVAVTM
                     CAIGTWAAFSLDTTVTYGLTALALISFVGSVSVARFRVPDTDKPDAVRKQR"
     gene            complement(11253..11792)
                     /locus_tag="VFC01_31885"
     CDS             complement(11253..11792)
                     /locus_tag="VFC01_31885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HZN84257.1"
                     /translation="MRRVALRAWVLFWLMLVWILLWGTLSAANVLSGLAIGLVITVLL
                     PLPVVPIEGRVHPLSVLRLVTTVGYYLVLSSVQVAWLAIKPGPPPFSAVLRAHVAVKS
                     DLVLALAVNIFNLIPGSIVLEIDQARRMLYMHVIGVGSDRAVNRFYRQVAKVERLLVS
                     AFERESDWQPAPREEADPA"
     gene            complement(11789..>12187)
                     /locus_tag="VFC01_31890"
     CDS             complement(11789..>12187)
                     /locus_tag="VFC01_31890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84258.1"
                     /translation="LVTTVGYYLVLSSVQVAWLLVGGGVLTSLLTLYVIVRVWTKAFW
                     RSREDAPEGHLAGAMPLALLDEPEDIQFVDRDQVGRMPIGMVVPTGALIAVGLTLTVM
                     AGPIFGYAERAADEVLDRGQYVSAVLGSRE"
BASE COUNT         2109 a         4013 c         3983 g         2082 t
ORIGIN      
        1 ggccgcctac gccggccatg tcgatgaggc tcgccgcgcc atagaagcgg cccttgctgc
       61 aagtcagcgt tgcgctgcaa accttttggt ggtctggacg atcaccacgc tgggattcct
      121 ggaagtgtca ctcggcaact acgacgcagc cttgacagca gtggcacccc tgctgtccag
      181 cctcgatgcg gcgcccgacg gaacggagat tccgttcgca tcgtttgtgc cggacgccgt
      241 cgaggcgatg attgcactcg gacggattgc cgatccggag ccgctggtga gcgcgcttga
      301 acgtaacggt gcccggctcg accgggcatg gatgctggcc atcggcgccc ggtgccgcgc
      361 catgctcttg gcggcgaatg atgacgtcgg cgccgcatac cgggccgctg aggccgcgat
      421 ggcccaccac gatcgtctgc cgatgccgtt cgaacgtgca cgcacgcagt tgctggtcgg
      481 tcagttgcag cacaggcaaa agaatcggca ggccgcaacg gccacactcc gggaggcgct
      541 gacgaccttc gaggagctcg gcacgccgct gtgggcggaa cgcgccaggg cagagttgaa
      601 acgggcctgc ggaatccgca cccgggccga actcaccgca tccgaacagc gcgttgccga
      661 actggcggcg acggggatca ccaatcgtga gatggcggcc gcgctgtaca tcagcccaaa
      721 gacagtcgag gccaacctgt ctcgcatcta ccggaagctc aacatccgct cgcgggctga
      781 gttggggcga atcatcggca aaaccgagca atagggaaac acccgattca gcggccctcc
      841 gactgtctta ctgtgacgcg atgagaccgc attacctggt cgagtggtac cggtccgcaa
      901 tcgccgacga gccgctcgac cacagcgtcg cacgcctgat ccaatgcgct gcgtccatgt
      961 ccgccgctgg gtcgccggtg aacctcctcg cgacgcttgc tgttcccacc gacgagatcg
     1021 tcctggcggt attcgattcc gcgtccgaac tgtcggtcgt cgaggtatgt cgtcgggcag
     1081 gctgtcccgc tcagcgactt actgctgctg tggacgcccg gttctggccg tcggcatagg
     1141 ctcgccgacg ttaagcggtg cgtcgattgg gtaccccggg gcgcatgggt aacagcgtcg
     1201 ataccaggcc ccgcagcgtc cgcgagcagg cgaaacggca cgcccacgaa gtgcgcacgc
     1261 acatggagga acgccgccag cagcaagatg acggactcac cggctggctg aacaagcgcg
     1321 caggtgactg ggagctcagc ggtcctgacg aggcgaccat gcagcgccag aagtatttct
     1381 ggaacttcct cgtcgaccac tggttccgga tggagttcga cggctgggag aacctgcccg
     1441 atccaccggt gcttctggtg ggcattcact cgggcgcgcc gttcgtatgg gacgcgtgga
     1501 ctgtcggggt gcagtggtgg cggcggtttg ggcaggacag gccgctgcat ggcaccgcgc
     1561 acgacgtgct gatggcgatg ccgctcgtcg gccgatactt ccgggcgatg ggagtgctgc
     1621 cggcggcgcc ggactcgatg gccactgcgt tggcggaggg gcgagatgtc gccgtctggc
     1681 ccggcggtga ggtcgactcg ctgcgcccgt ggaccgaacg cgaccgcgcg aacctggccg
     1741 gccgcaaggg ttttgtgaag atggcgatcc gcgccggggt accgatcgtg ccgatcgcga
     1801 ctgtcggcgg tgccgacgcg atgccggtgc tgatccgcgg tgatcagctg tctcgatctt
     1861 tacggctgga taagctgttg cggctcaagg tctttccgat tgcagcctcg ctcccgtggg
     1921 gtatcgcacc tgccgcgttg ccgcagttcc cgctgcccgc caagatacgc acccggctga
     1981 tgcctgccgt cgagttggat cacgatccca aacgtgccga cgacgacgag tacctcgaag
     2041 agaagtattg ggaggtacag gacagcatcc agcagggcat ggacgcgttg gcgcgcaagc
     2101 gggcgttgcc attgttcggc tgagatcgtc aaagttcacc gaagtagctg tgatgcactg
     2161 cgcgtgcggc taggctgata gcggccacag tcgccatcgg gggtgtgcag gtgaggcatc
     2221 aatgagcatg caaccgcaag ggccgggttg gtggcgggct tccgacggcc gatggtaccc
     2281 accgcaaccg cgtccagcct atccaccgca accgtttggc gcgggacccc cagtgccaca
     2341 gaagaagtcg ggcggtcacg gcgtgttgat cgccgttctg atcgtcgtcg ccgtcgtcct
     2401 cggagtggga gtgctgggcg tctacttcgt gtacaggaca gtctcgaact ctgtctcgga
     2461 cgccgtgggc ggcggagagc tgaattgccc ttctgcggaa gatatctcaa cacttgtcgg
     2521 ttcggaggtg aacggaccca cgggcggaaa catcgttgtg gcaagcggat gctattacct
     2581 gccgaagggc cctgatgaca cgatcgaagt gatcatcgtg tcgggatcca agctcatcgc
     2641 cgacgacgaa atcaggtcgt tcgagagcga gggtcgagcg gcgaatgtag atgtccgatc
     2701 aatcgatgtc ggcgaccgtg gccacgcttg ggcctcgtca accaagagtg cagcgatcgc
     2761 ggtcggcgaa caatcgctcg tgagcgtgga ggttcagggg aaggacttca ctgcgatccc
     2821 cgacaagacc gacgcggcga tcgcgatcct ggagagggtt ctacattgac ccgctgatcg
     2881 ttgactctgc ggtcagggcg cgacctactc gcatttttgc gccctggccg cagagtcgat
     2941 ggttatgtca actccaccaa cactggcgcg tggtcgctcg gggccttgcc tttgcgctcc
     3001 tcgcgcacga tctcggcgtg cgtgacgcga tcggctaatg ccggcgagcc gaggatgaag
     3061 tcgatccgca tgccgcggtt tttcgggaac cgcagctgcg tgtaatccca gtacgtgtac
     3121 accgcaggcc caggcgtgaa aggccgcacc acatcggcga attgagtgtc gacgatcgcc
     3181 tggaacgccg cacgctccgg tggtgtgaca tgcgtgctgt tcgcatagaa ctctggactc
     3241 cacacgtcct cgtcggtggg tgcgatgttc cagtcgccga cgagcgcgat ctgcgccgac
     3301 gggtcatctg tcagccacgc ctgcgccgta gcacgaaggg cggcaagcca ttccagcttg
     3361 tagtagtagt gcggggagcc gacgaatcgc ccgttcggta tgtacaggct ccaaacttgc
     3421 acgccgttac acgtcgcgcc gagcgcacgt gcttccgccg cggcctcgac atccggcttg
     3481 tcgctccata ccggctggcc atcgaagccg acttgaacgt cgtcgaaacc gacccgcgac
     3541 gcgatggcca ccccgttcca ttggttgaac ccgcagtgca cgacctcgta gccggccgcc
     3601 agaaacggca ttgcagggaa ctgctcgtcg ctgcacttgg tctcctgcat ggccagtacg
     3661 tcgacgtcgg cgcgctcgag ccagtcggtg acccggtcaa cgcgggcgcg gatcgagttg
     3721 acgttccacg tggccaaccg catggcttac aggctacggc cgtcgagata tcgcgagcgg
     3781 tggtgaacgg tgaagcccat cgactcgtac agctgtgtcg ccgcggtgtt gtcggcgagc
     3841 acttgcacat aagcgcgcgt cgcaccccga tcgccgcccc aacccaacag cgccccacaa
     3901 agttcgcggg ccaggccgcg acgccgtgct gcctcgacga cgtgaacagc tgacaacccc
     3961 acccagcgtg tgccgtcggg cgccgtggtc acggcggccc ttccaactga cgcatcgccg
     4021 accgtcgcga atgccacctc gccgtcgatc actgctgtca acacgtcgac cggcacctcg
     4081 cgctcataca gccgaagcca gtcgtcgtcg ggcgcggttg cgattgtgac ccgagacgaa
     4141 ccctgcaccg ccacatcgcc ggtcatgacc aggttttcgg cgccggtcga tacggtgggc
     4201 gggatgcgga acaaccgatc cggtgcggct accagcggcg ccaccccatg ggccgcatac
     4261 cagtcgatga tttcgctgac atcgtcgcta gtcgaaacat tcagcgggac agctgaattc
     4321 gcgcgccggg tgcggccgtg gccgaagcgc agcaaccaac ccccaagcca ctgatgttca
     4381 gcacccggcc atgctagtgc cgccgcatgc tcgaggttgc ggatgtcgcc ggtccgtacc
     4441 ggagccgcgg caagggcttt gatcgctacc acgtcgtcgg ccgcgatcgc caccacgtca
     4501 ccgcgtttgg tgcgcacccg cagcgtcggg ccgacatcca acagctgacc gaccacgtcc
     4561 gtcagcggtg gcgccgagcc ggcagggagc cggtagcgga ccacgacgcg cgtgccgacc
     4621 tcaggcacta gtggccgaac ggatcgggat ccttgccggg catccaggtc agccccggca
     4681 cgccccagcc gtgcgacttc accgaccgct tcgcgctgcg cgcgtgccta ccgaccaacc
     4741 tgtccagata caggaagcca tccagatgtc cggtctcgtg ctgcagcatc cgcgcgaaca
     4801 ggtcggtgcc ttcgatcgtg atcggggtgc cgtcggcgtc caagccggtg acgcgcgccc
     4861 atttggcccg ccccgtcggg aacgattcgc cgggaaccga caggcagcct tcgtcgtcgt
     4921 tctccgggtc aggcatggtc tcgggcactt cagttgtctc aagcaccgga ttgacaacaa
     4981 ctccccgccg ccgcgtcgtt cgaccgcgct cgtccgcgca gtcgtagacg aacacgcgct
     5041 tcgaaacgcc gatctggttc gcggccagtc ctaccccgtt ggcggcgtcc atcgtctcgt
     5101 acaggtcctt gatcaactgc acaacgtcat ccggcagcga accgtcggcg ttgacagtca
     5161 ccggttcggt cgcggtgtgt aagacgggat ctcccacgat tcggatgggt acgacggcca
     5221 tcgggaaagc ttaagtcggc aacctttgtg agccgactcg cggggttggg tgacgtctta
     5281 gcctccaaac ccgtgattga atgttcgccg aaccgtcggg atttcatttc gagtgtgggg
     5341 aagggtccag gggcaatatg gacggcgcga tcgcgcgtac tgagcaatcc ggggacgact
     5401 ctgatctcac cgatgggctg actcgacgcg agcacgacat cttggcgttc gagcgccagt
     5461 ggtggaagta cgccgggtcc aaggaagacg ccatcaagga gctcttctcg atgtcggcca
     5521 cccggtacta ccaggtgctg aatgcgttgg tggatcgccc ggaggcgctg gcagccgacc
     5581 cgatgctggt caaacggctg cggcggctgc gggccagtcg gcaaaaggcc agggcggcgc
     5641 gtcggctcgg cttcgacgtc acctgaggtt ttctggcggc accggccgtc tcgatacagt
     5701 gagtggtaat gaaccagcga aattcctccg ggctgcctct gcgcgccatg gtgatggtgt
     5761 tgctgttcct gggcgtggtg ttcctgctgg tcgggttcca ggcgatgggc tccggcgact
     5821 ctgagggcga ccagtcgtcg tcgatcgcca ccagcacgat caccacgacg agttcagcga
     5881 cgacgtcgcc atccccggcg ccggcgaggg ccgatgtgcg ggtctacaac atctcgtcga
     5941 ccgaaggcgc cgccgaaacc accgccaacc ggctgcgcga agccggctgg aacgtcaccg
     6001 agaccggcaa cctgagcctg cccgaggtca cggccaccac ggtgttcttc agtgacgcgc
     6061 ccggcgagca cgaggccgcc gacgaggtgg gcaagttgct gcaggcgccg gtgcagccgc
     6121 ggctccccga gctgaccgag caaccgccgg gcgtggtcgt ggcggtcacc ggctaggctc
     6181 ttgcccatgc tgaagaccgt cgctgctgct gccttcttcg ccgttcctgt cgtcgcccta
     6241 agcgcgtgca gcccgaacga acccatcgcc acctcacccg gcaccacgcc atcggtctgg
     6301 accggctcgc cgtccccgtc cgcgtcgccc ggtgaggccg aaggtggaca cggctccgag
     6361 ggacaagccg cgagcggcga gaagctcact gcgcagttga aactcgccga cggcaccgcg
     6421 gtcgccaccg ccgacatcga attctcgggc ggcttcgcca ccgtcacggt ggagacgacc
     6481 acaccgggca agctgacgcc gggcttccac ggcctgcaca tccacgccgt cggtaaatgc
     6541 gaagccagct ccgtcgcccc gaccggcggc gcgccgggtg acttcaattc cgcgggtgcg
     6601 catttccaga agtccggtca cacaggccat cccgcgagcg gcgacttgag ctcgcttcag
     6661 gtccgcgagg acggcaccgc gaagttggtg acgacgaccg acgcgttcac ggccgaggat
     6721 ctgctcggcg gcgccaagac cgcgatcatc atccacgaga aggcggacaa cttcgccaac
     6781 atcccgccgg agcgctatca gcaggcgaac ggcgcgccgc cgcccgacga gaccaccttg
     6841 gccaccggcg acgccggaaa gcgcgtggcg tgcggtgtta tcggcaccgg ctagcagcca
     6901 catagacttc gccggctcgc cccggccgac gcttggcgtc gagtgggagt tcgcgctcgt
     6961 cgacgccgaa acccgcgacg tcagcaacga ggccgcggag gtgatcgtcg agctcggcga
     7021 gaacaacccg cacgtccaca aggaactgct gcgcaacacc gtcgagatcg tcaccggcat
     7081 ttgtgattcc gtgccgcagg caatggagga tctgcgctcg acgctggtga ccgcacgcaa
     7141 gatcgttcgc gaccggggca tggagctgtt ctgtgcaggg acgcatccgt tcgcgaaatg
     7201 gtcgcctggc agcctcaccg acgcgccgcg ttacgccgag ctgatcaagc gcacgcagtg
     7261 gtggggccgc cagatgctga tctggggtgt gcacgtccac gtcggtatct cgtcggcgca
     7321 caaggtgatg gcgatcaaca cgtcgttgct caaccattat ccgcatctgc tggcgctttc
     7381 ggcgtcttcg ccgttctggg acggcgagga caccggctat gcctccaacc gcgcgatgat
     7441 gttccagcag ctgccgacgg ccgggctgcc gttccacttc cagacctggg cggagtggga
     7501 aggctttgtg cgcgaccaga agaagaccgg catcattgac cacatgaatg aaatccgttg
     7561 ggacatccgg ccttcgcccc ataaagggac ggtggaggtg cggatcttcg acggggtttc
     7621 caacattcgt gaactctcgg cgcttgtggc attgacccac tgcctcatcg tcgacctgga
     7681 tcaccgactc gacgccggcg agcggcttcc gaccatgccg ccctggcatg ttcaggaaaa
     7741 caagtggcgc gcggcacgct acgggctgga cgcggtcatc atcctggacg aagacagcaa
     7801 cgagcgactg gtcaccgacg atctcgacga gctgctgaac aagcttgagc cggtggcgaa
     7861 gtcgctgaat tgcgccgacg aacttgcccg ggtctccgac atctaccggt tgggcggttc
     7921 ataccaacgt cagcgccggg tcgccgagga gactgacggc gatctgcggg cagtggtcga
     7981 cgcgttggtt ggcgagctgg aactctagcg gtgcccatca cgccgatgtt cccgctggag
     8041 gtggccatgc tgcccggcga ggagctgccg ttgcggatct tcgaaccccg ctataccgca
     8101 ctggtgcagg actgcctggc ggctgatgac cctgcgttcg gtgtggtctt gatttcggct
     8161 ggccgggagg tgggcggtgg tgacacccgc agcgacgttg gcgcgctggc gcatatcacc
     8221 gagtgcgccg acttcggcga cgggcgttac cggctgcgct gcgtgatggc cgagcggatt
     8281 cgggtgctcg agtggcatcc cgatgatccc tacccgcggg cgatggtgga ggtctggccc
     8341 gacgagcccg gcccggcggt cgacgtcgag gcgatccgtg atgtcgagga tcggatggcc
     8401 gcgttgttcg agcgaatcgc gagggttcgc ggcgccgagg tcaatccacg ggacatcgtc
     8461 gcgggtgccg acgcatcagg tgatgccgca atgtggttgt acgcgttggc ttcccgcgta
     8521 ccgatgggac aggctgaccg gtatgcggtg ctctcggcgc ccacggtcgc cgcgcggacc
     8581 gcagcgcttc gcgacgcggt cgagaccgtc accgcgatgg tcgagtttga gctttcggag
     8641 tagctaacgc gtaagcacgc accatgctgc cgccggaaag gtgtatgaat cgtcctcgtt
     8701 tcggtagggc tcggcgttcg cctcgattgc cgctcgcgtt gccgaccgtt cgtcctcggg
     8761 cagcgcaatg atcacctgcg ccagcgggcc ggcgaggtgg accagaaagt cccacaagtc
     8821 gtcgaaatcc tggaagggga agtcgagggc gacctcctcg agctccggct caccgaaacc
     8881 ggtctccgtg acaagctcgc tgattcgggc cggctccgcc aacgcaaaga tccctggtgc
     8941 tcccggttcg ggcgctggca tgtgacctcg ctgcacaagg gtcatcccgg gcaccgccgc
     9001 ccacgggttg gcgtcggcag tccgccagac ggcgaaagcc aacgggccgc cgctgcgcag
     9061 cactcgccgt gtctccatca gggccaccgc cgggtcggcc atgagcatgt agccgaagcg
     9121 gcataccact ccgtcgacag tgtcgtcgtc gatgtccatg ctctcagcgt cgagcactcg
     9181 gtactcgacg ttcgccaggc cgcgggctcg gccctggcgt cgggcgacat cgatcatctc
     9241 tcgtgcgaag tcggtggaga ttacgcggcc tagttggccg acgtgttccg ccacccggaa
     9301 gcccaggtcg cctgtcccgg cggcgatctc gaggatcgtc tggccgccgt gagggtcgag
     9361 tttgccgacg agccagtcgc tgaccggtgc ggtcatctgc atcagccact cgcggcggtg
     9421 ctcccaaccg ggggccatct ggccccacgt gtgtaggctc tgctcccgat atgtatcgcg
     9481 atccattcgc gtgctcccct ggatgtcccc gagagtgcgg ttgttgacgc tctcactcgc
     9541 gatttgcgtc aacaatcgca ctctcggcga gggggatcat cgttttcccg catttcgttc
     9601 gcggtcagca gaccagatgg ccctcgacga acaccgtgcg gggggcgacc gcgtcgtcct
     9661 ggtccggtga gcagcggccg tatcgcgtca tcagttcgac tgcctcgacc gccgtgacct
     9721 cccagttgtg ctcggtcagc cactcggcca catcggtgcg gtcctcgacg taccacagat
     9781 cctgggtgtc gggaacgttg gcgtcgggat cctcgcccgc ctcttcgcgc aagcgccgca
     9841 attgttctcg acggctggcc agatagtccc gatcgaagaa gccgcggccg aacgactcga
     9901 ctccgatgcg gctgccctcc gcgctgaggt caccgatccg ttcgaacagc aggtcctgtc
     9961 cggcggcggg caggtagggg agtaggcctt cggccgccca tgccgtcggc accgtcgtat
    10021 cgaatccggc gtcgcgcaac gctttcggcc aatcatgcct caagtcgacc ggcactgcga
    10081 cgtagcgaga cacactgggg gtggcgtcgt gctgggccag cgtctgggcc ttgaactcaa
    10141 gcaccttggg ctgatcgatc tcgtagagca ccgtgtcgtc cacccacggc agccgccagc
    10201 cccgggcatc caggcccgcg gccaggatga cggcttggcc gatgccgtgg gcgccggccg
    10261 cgatgaagaa ttcgtcgaac cacttggtgc gcgacgcggc gtacgcggag atcgaccgga
    10321 tccgctcgag catgtacgac ggcggttgct gccagccgcg ctcgaccgcg gcatcgagaa
    10381 acagctgcgc ataagggtcg atgaacagcg ggcactcgga cgtcgactcc tgcgaccgcg
    10441 accaagccac accgagcgcg gtctggccta cgccttcggt gatgtcccag ctgtcgtcgt
    10501 cagttctggt catcgccacc gctttcccga ttttcggcga actcaagcat ttcgtccttg
    10561 gtcaggaggt catcgcgaat gttctgctcc cggtacgcga gttgtccgac gcgattggcc
    10621 accaccggcg cggtgatcac ggtgaacaaa ccggtgagga tcaccatgcc gacgtcggca
    10681 tggccgcgca gccggatcgc cgcgcctacc agcaccagca acaggcccag cacctgaggc
    10741 ttggtcgccg cgtgcatccg ggtcagggtg tcgggaaagc ggaccacgcc gatcgcggcg
    10801 gtcagcgcga gcgtcgagcc gcccagcacc agcacagcgg tgacgatgtc cagtgcgctc
    10861 atcgctgttt ccgtaccgcg tcgggtttgt cggtgtcggg cacccggaat cgcgcgacgc
    10921 tgaccgagcc gacgaaactg attaacgcca gcgcggtcag gccatacgtc acggtcgtgt
    10981 cgaggctgaa cgcggcccat gtgccgattg cacacatcgt caccgccacg agcgtgtcca
    11041 gcgcgacgag cctgtccagc gtgctcggcc cggcgagcaa ccggaacata gtgatgaagg
    11101 ccgcggccgt gagcatgacc gcggcgatca cccagacaat tgtcatgcct cggctcccgc
    11161 tcgctccggt cctcgcttcg ggcgggagct tgcggttcgc cccatcgagc ccggtctcgc
    11221 tgcgatcctc actcgcggcc gccttcgggt cgtcatgcgg gatcagcctc ctcccgcggt
    11281 gccggctgcc agtcggattc gcgttcgaac gcggacacca gcagccgctc cactttggcg
    11341 acctggcggt agaaccggtt cacggcacgg tcggagccga caccgatgac gtgcatgtag
    11401 agcattcggc gggcctggtc gatctccagc acgatcgagc cgggaatgag attgaagatg
    11461 ttgaccgcaa gtgcgagcac gagatccgat ttgaccgcga cgtgggcccg cagcacggcc
    11521 gagaatggcg gcggcccggg tttgatcgcc agccacgcca cctgaaccga ggacagcacc
    11581 aggtagtagc caaccgtggt gacgagccgc agcactgaga gcgggtggac cctgccctca
    11641 atcggcacaa ccggcagcgg cagcagcacc gtgatgacca gtccgatcgc caggccggag
    11701 aggacgttgg cggcagaaag cgtgccccac aacaggatcc acaccagcat cagccagaac
    11761 agcacccacg cccgcagcgc cacccgtctc attctctgct ccccagtact gcgctgacgt
    11821 actggccgcg gtcgagcacc tcgtcggcgg cacgctccgc atacccgaag atcggacccg
    11881 ccatcaccgt aagcgtcaag ccaactgcaa tcagcgcgcc cgtcggcact accatcccga
    11941 tcggcatccg ccccacttgg tcgcggtcga cgaactggat gtcctcaggt tcgtcgagga
    12001 gcgccaaagg catcgcgccg gcgaggtggc cctccggagc atcttcccga gaccgccaga
    12061 acgcctttgt ccatacccgg acgatcacgt agagggtcaa caggctggtg agcacgccgc
    12121 cgccgaccag cagccacgcc acctgaaccg aggacagcac caggtagtag ccaaccgtgg
    12181 tgacgag
//
LOCUS       DASRJA010000707         9268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2918434,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000707 DASRJA010000000
VERSION     DASRJA010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9268
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2918434"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1299)
                     /locus_tag="VFC01_31895"
     CDS             complement(193..1299)
                     /locus_tag="VFC01_31895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN84259.1"
                     /translation="MTRFAFEVLRNLGVAVTAAVTALAVVLLVHAVTVNEPAFAASTA
                     RARATADLSSYWVERVAAKVLPSVVTLQIGDDDNYVVGSGVILDANGLIMTNYHVVAA
                     ASQAPRGSVMRTAVTLNDGRTAEFDIVAADPQIDIAVVRARHLSGLTPISAGSSATLR
                     VGQPVVAVGSPLGLAGTVTEGIISALNRPVCPRVLADHGVVAYEAIQTDAPINPGNSG
                     GALVDLNGRLIGINSAGSTLGNAETAALTPQGSIGLGFAIPVQPAMRIAAELIATGHA
                     SHGWLGARVSSDMATRGARIVDVAAGSPAAAAGLTPGALVTEAGDHVIDSGNALIAAV
                     QSRAPGTSIALAFSDTAGNHRAVEVTLGTDEGRD"
     gene            complement(2256..3437)
                     /locus_tag="VFC01_31900"
     CDS             complement(2256..3437)
                     /locus_tag="VFC01_31900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HZN84260.1"
                     /translation="MSQLPIDPDANGSRGAGMPCSNCDHGRDRPICRNKHTCQTHGPA
                     QNGEAVVATISLGGHAGEIATCPDGNQIYVIVDDSVKVINRLHHIVGSYRTGPHPKSL
                     LVSADGTRVYVTGYDGSTSVITTADNTVKTFKLERSNAEVVSPDGSHIYRAHSGIVGD
                     TGGSWISVVNAEGATVAVIPVDRHATDIALSPDGRRLYISSRRGASYLDARGLISVID
                     TSTYSVVDKIAVELAADSMTMSFDGTRLYSTHYHKNAISIIDLRTCAVSRWMFGDAPI
                     DIAVSPDDAIACVTNLQSLTVFNAAANIVKSAVVGALPRGTCLSADGKRAYVLDFVRR
                     TILALDTADNVVVGSADVDGHPEAMALAPDGEFLYLTDYPNGVVTVISTALLKRTAPG
                     R"
     gene            4115..4843
                     /locus_tag="VFC01_31905"
     CDS             4115..4843
                     /locus_tag="VFC01_31905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN84261.1"
                     /translation="MESGGPAGTRLRVVVAEDDTLLREGLASLLERSDFEVVGQAGDA
                     IDLLPLVREKSPDLVVVDIRMPPTNTTEGLDAARQIRLESPDIGILVLSAHVEVEHAM
                     DLLEGGHKAGYLLKSRVGDVADFIDSLKRIARGGTVVDPELVKELVSERRRHDPLAVL
                     STREREVLALMAEGRSNAGIGRELWVTEGTIEKHVSSILTKLDLPETANDHRRVRAVI
                     AFLDAPPASRESAAATSASPTPRR"
     gene            complement(4934..5350)
                     /locus_tag="VFC01_31910"
     CDS             complement(4934..5350)
                     /locus_tag="VFC01_31910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN84262.1"
                     /translation="MGSTVCVRDTGGMASGVEELRCVIVDDNPVFIEVATRLLERGGI
                     SIIGAASTIAEAIQRVEELRPDVTLVDVDLGGESGFHLAVKLHQPNSHAGPKIILTSA
                     HSEQDYADMIAASPAVGFLAKADLSPDAIHQLLAAS"
     gene            5735..6265
                     /locus_tag="VFC01_31915"
                     /pseudo
     CDS             5735..6265
                     /locus_tag="VFC01_31915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            6262..7044
                     /locus_tag="VFC01_31920"
     CDS             6262..7044
                     /locus_tag="VFC01_31920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN84263.1"
                     /translation="MRLGRSPSAYVGADPSHSIARARRHRAPRGAGAQLRDTIVSAAT
                     ELLIETGYAKAVSIRSVAERVGVTSPAIYLHFADKDALLDAVCAQYFQKLDGQMRSAA
                     AGCTSTLEVLHALGMAYVRFALKSPVLYRIATMGDPRSPSDVDTVLANSAFVHLRAIV
                     QTMIDDGTYPQRDAHKMALELWTVVHGVAALLVAKPHWLLDDVEELTDKVLRAMCYGQ
                     IAAGIVGYDKSASDAIDHLMRLKSESSPFGASNSRTRPPAGS"
     gene            complement(7016..8842)
                     /locus_tag="VFC01_31925"
     CDS             complement(7016..8842)
                     /locus_tag="VFC01_31925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN84264.1"
                     /translation="MGHYIANVRDIEFNLFEVLNLGAVLDSGGYGDLDVDTARTMLEE
                     VARLAEGPVAESFAAADRNPPVFDPERHTISVPGELAKSVQAIKDAEWWRVGIAEGIG
                     GVPAPAPLVWAVHEMLLCANPSAPFWWALGPAMAHALFIEGNDQQKRWAAMGLERGWA
                     ATMVLTEPDAGSDVGAGRAKAIAQPDGTWHIEGVKRFISGGDVGDTAENIFHLVLARP
                     EGAGPGTKGLSLFYVPMYLFDPDTLELGPRNGVFATGLEHKMGLKSSPTCELTFGAHG
                     IPAVGYLVGDVHNGIAQMFTVIENARMTIGVKSSGTLSTGYLNALAFAKERVQGADMT
                     QMTDKTAPRVTIINHPDVRRSLMTQKAYAEGLRALYLYAAAHQNDDVAHHVSGADPSM
                     AHRVDDLLLPVVKGVGSERAYELLTESLQTFGGSGFLQDYPIEQYIRDAKIDSLYEGT
                     TAIQALDLFFRKIVRDRGEALAHVAAQISHTIDSCDAELKTQAEQLRTALEHVQAMTA
                     TLTGYLMAAAEQPAEIYKVGLGSVRFLLAVGDLIIGWRLLVQANVAQTALAATKGDEG
                     FYRGKIATAKFFAANVLPNLAVLRGIVENLDDEIMSLPEAAF"
     gene            complement(8973..>9268)
                     /locus_tag="VFC01_31930"
     CDS             complement(8973..>9268)
                     /locus_tag="VFC01_31930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HZN84265.1"
                     /translation="IVFETALEPRLYVDPSLVRTDLLRRTDTSTYCNYKGYATYWAAV
                     VGDTVVDDVAWSYEDPPPECLPIKGYLSFDAARAEVLAELPAAGQAPDCGCEV"
BASE COUNT         1732 a         2888 c         2935 g         1713 t
ORIGIN      
        1 tgatggtgtg gggggagccg aattttccgg gggcaccggc cttatgggtt tgaaggaccg
       61 agtcgacgcc ctgggcggcc gtatcgattt acacagtccc cgaggagggg gaaccacatt
      121 gcatgtcacg cttccgctca accgggccga acattgaaaa gttcagggct cgggtagcgc
      181 cgagggccgc gtttagtctc gcccctcgtc ggtgccgaga gtgacctcaa cggcgcggtg
      241 gttacccgca gtgtcgctga acgccagggc aattgaggtg ccaggcgccc ttgactgcac
      301 cgcggcgatt agggcattgc cgctgtcgat gacgtggtca ccggcttcgg tcaccagcgc
      361 gccgggagtg agcccagccg ccgcggcggg gctaccggcc gccacgtcga cgattcgagc
      421 accgcgagtg gccatgtcgc tgctcacccg cgcgcccagc cagccatgcg acgcgtgacc
      481 tgtggcgatt aattcggcgg caatacgcat ggcgggttgg accggaatcg cgaaacccag
      541 gccgatcgac ccttgaggtg tcaatgccgc ggtctcagcg ttgcccaatg tggacccggc
      601 cgagttgatg ccgatgagcc gaccgttcaa gtcgaccagt gcgccgcccg aattgcctgg
      661 gttgattggc gcgtcggttt ggattgcttc gtatgccacc acgccgtggt cggcaagtac
      721 gcgtgggcat accggccggt tgagggcgct gatgatgcct tcagtgacgg tgcccgccag
      781 gcccagcggc gatcccacgg ccactactgg ctgaccgacg cgtagggtcg ccgaggagcc
      841 tgcagagatc ggggtaaggc ccgacaaatg gcgggctcgc accaccgcga tgtcaatttg
      901 cggatcggcc gcgacaatgt cgaactcggc tgtgcggccg tcgttcagcg tgacggcggt
      961 tcgcatcacc gacccgcgcg gggcctggct tgccgccgcg acgacatggt agttggtcat
     1021 gatcagccca ttagcgtcga ggatgacgcc agagcccacg acgtaattgt cgtcgtcacc
     1081 gatctgcagt gtcacgacac tgggcaagac cttcgcggcg acccgctcga cccagtagga
     1141 agagaggtcg gccgtcgccc gcgcacgcgc agtgctggcc gcgaacgcag gttcgttgac
     1201 agtgaccgca tgaaccagca gaaccacggc cagagcggtc accgcggccg tgaccgccac
     1261 tccgagattc cgcaaaacct cgaaggcaaa ccgagtcact ctttgcctct gcgtggtcac
     1321 ggggatcgcc cgatgagacg gggatctccg ggcgggcttg cgatctggct gtgtagccac
     1381 ggtatgccgg ccagcatgct gagctcgaga tctgcgcatc atccggccct catcacccgt
     1441 cgtggacaac ggcggcaatt ggcactgaca tcggtttctc ctcaatttca ctttcgcttg
     1501 tgcgcgccgt gcgcccgcac gtcgaagtcc gagcgacgca acgagatcgg ctgtcgctca
     1561 gaatgccggg cgtcgaatgt gtctgtcctc accggcaacg ccaatcgcga tacccggcta
     1621 gcgggtagcg tggaatgcgc agcatcgctg acaaacgccc aaaaggtgac tcaggcaagg
     1681 aataacattc gccgcgccga tggctccgac gtgcagccga cggatttgga ccggtacgcc
     1741 gaaccggtca acgccgtgag ctattgcgag tctctcgcag tcagtctcgc cgaaagcctt
     1801 gtgtcaaaac ggcattgaaa acgaaacaat ccaaaaccga tgaggtgtgg gggactcctc
     1861 tagcgcaggc cgccgggtcc tgacggcagt ggtcctctcg gcgccaccgg cggggctggc
     1921 ggggctgccc cagccgtagg gtccttatcg catatcccac gggcccgagg gggctgccat
     1981 tgcttgtcgc agggataggg ggttgctgga tccgcgacgg ctggcggcgc gccgataagc
     2041 acagttgccg ccgaagctgc caccagtgca gctcccacat agcgccgtat tctcatggtt
     2101 ttccttgtcg agtcgttgat ggctggctgt gatgtctccg cggcgagaat gcttagcatt
     2161 gccgctacgt ccagaatccg cgttgccaca acagatgtca tcgccgctaa cgcccatccg
     2221 gacatcgggc tagcagtaag aagggaaggg cgttactacc ttcccggcgc cgttcgcttc
     2281 aacagtgccg tcgagatcac ggtcacgacg ccgttcggat agtccgtgag gtaaaggaac
     2341 tcgccatcgg gtgcgagcgc catcgcttcc ggatgtccat cgacatcagc actaccgacg
     2401 actacgttgt cagcggtgtc caacgccaga atggttcttc ggacgaaatc gagtacgtag
     2461 gcccgctttc cgtcggcact gagacaagtc ccgcgaggca aggcccctac gaccgcgctc
     2521 ttgacaatgt tggcggcggc gttgaagacg gtgagggact gcagattggt cacgcaggcg
     2581 attgcgtcgt cggggcttac ggcaatatct atcggagcgt caccgaacat ccaccggctc
     2641 acggcgcatg ttcgaaggtc gatgatggat attgcgttct tgtggtagtg ggtggaatac
     2701 agcctggtgc catcgaagct catcgtcatg ctgtccgccg ccaactccac cgcaatcttg
     2761 tcgacgacac tgtaggtgga cgtatcgatc accgagatca agccgcgcgc gtcgagatac
     2821 gaagcgcccc ttcgagacga gatgtatagc cggcggccgt ccggacttaa cgcgatgtcg
     2881 gtagcgtgtc ggtcgaccgg tatgacagcg acagttgcac cctcggcgtt gacgaccgag
     2941 atccagctgc ctccggtatc cccgacgatc ccgctatggg ccctgtagat gtggctccca
     3001 tcagggctga caacttcggc gttgctccgt tccagcttaa aggttttcac tgtgttgtcc
     3061 gcggtcgtga tgaccgacgt cgacccgtcg tacccggtga cgtagacgcg cgtcccgtcg
     3121 gcactcacga gaaggctctt cgggtgaggg ccggtgcgat acgagccgac gatgtggtgc
     3181 aaccggttga tgaccttgac cgaatcatcc acgatgacgt agatctggtt tccatcgggg
     3241 caagtcgcga tttctcccgc gtggccgcca agcgagatcg tcgcgacgac cgcctcgcca
     3301 ttttgggcgg ggccatgggt ttggcaggtg tgtttgtttc ggcagattgg gcgatcgcgg
     3361 ccgtgatcgc agttggagca cggcatccca gcgcctctgc tgccgttggc gtcaggatcg
     3421 atgggcaact ggctcatggg ttctgcctac cgggcttcgg ccggtatcgg cgagctgatc
     3481 atcgactcac caccgttacg tgtctgcgcg acacgatcac tagctcgcga gcaccgctgt
     3541 ctcgagtcac ggtcaaggcg tcggggtaaa gcgccgtcac atatccgcag aagccccgtg
     3601 gactagcgtc ggagacgtga acgcgcgagc cgcgagccgg cgtgatgccc accaccgcgg
     3661 agccgctgac cacgtcacga acgtccatcc tgaaagcatt gccctcccag cttgaaattt
     3721 cgtcagcgct gacgccaatc accgcatcga gatagcgcca acttgccgta ctggttaaca
     3781 ggtatcggct tggtcaatgc gcatctattg cggcgtagag ccggtgctgg gcggcagcgt
     3841 cacttgccat gccgtgactg ctgcagcggt tgctgcctag gcgagctgct accgttggca
     3901 cagcacactc ggtgctagcc aagggcggat tcaaaggcta cgagacatcg cttccgcggc
     3961 cgcgcattgc gctgccccgt tggctgggat tacccccggg tacctccagg gacggcatcc
     4021 cagccacctc caattcttag gattagcact tccgcagtga cgtcagtgct gggatcgtta
     4081 aggaatggac caaagaagca cggactctac cgcgatggag agtggcggcc ccgcggggac
     4141 gcggttgcgt gtggtggtcg ccgaagacga taccctgctg cgcgaaggac ttgcgagtct
     4201 gctcgaacgc tcggacttcg aggttgtggg ccaggctggc gatgctattg acctgctgcc
     4261 gctggtgcgc gagaagtcac ccgatttggt cgtcgtggac atccgcatgc cgccgacgaa
     4321 caccaccgaa gggctcgacg ccgcgcgcca gattcgactt gagtctcccg acatcggcat
     4381 tttggtgctg tcggcgcacg tcgaagtcga gcacgcgatg gatctcttgg aaggcgggca
     4441 taaggccggc tatttgctca agagccgggt gggggacgtg gccgacttca tcgacagcct
     4501 caagcgcatc gctcgagggg gcacggtcgt cgacccggaa ctcgtcaagg agttggtgtc
     4561 tgaacgacgc cgtcacgatc cgctggccgt gctgagcaca cgcgaacgcg aggtcctcgc
     4621 cctgatggca gagggacgtt ccaatgcagg catcggacgc gaactgtggg tgaccgaagg
     4681 caccatcgaa aagcatgtga gcagcatcct caccaaactc gacctgcccg aaaccgccaa
     4741 cgaccaccgt cgggtccgcg cggtgattgc gtttctggac gctcctccgg ctagtcgtga
     4801 atccgccgcc gctacatcag cgtcgccgac acctcgtcga taacaaccgt tgcggtgcgc
     4861 cggcagccag cgatcgatct caaggaaacg gcagcgagcg catcggcatc gagcaagggc
     4921 cgcccgcacc gtcctagctg gcggcgagca actggtggat ggcatccggc gacaggtcag
     4981 ctttggctaa gaaccctacg gcggggctgg cagcaatcat gtcggcatag tcctgttcag
     5041 agtgcgccga ggtcaggatg attttaggac cggcgtggga attgggctga tgcagcttca
     5101 cggcgagatg aaaaccgctt tcgccgccca gatccacgtc gacgagggtg acatcggggc
     5161 gcaactcttc cacccgctgg attgcctcag cgattgtcga ggcggcaccg ataatcgaga
     5221 tgccgccacg ctccagcagt ctcgtggcta cctcgataaa tacggggttg tcatcgacga
     5281 ttacgcagcg caactcctct actccagacg ccatccctcc agtgtcgcgc acgcacacgg
     5341 tgctgcccat tggggctagc tgcaaaacgt tagtaggtct cgtcgttcta ttttcgccat
     5401 ggagaggtca caccgagttg tatggccggc gcttacgcca ccgcccgaat agatgctgac
     5461 cgattggcgt cagctcggca tcaccgccac cacctataca cgcttgcagc caacaggtaa
     5521 ttcggtagcg actcggcgat cgtttgcatg atcaacaagt ccttaccggc tagcagctat
     5581 tcgccactgt tgctgacggc cggtgttgac gggcgctggc cccggcgaca tcagtgcccg
     5641 gcgaaggcag tctgagacag tgcaaacgga tactggggtt cgaccgcagc gcggcagaga
     5701 gcggagcatg gacgtgcccg attctctgca cgaacggttc gagcgtgatg tcgttccact
     5761 ccgggagtcg cttcaccttc atgctttacg gctgacgggg aaccgcgacg acgcggagga
     5821 tttactgcag gacaccatga tgaaggcgta tgccggattt cacttgtttc gacaggggtc
     5881 ttatctcaag gcatggctgc accgaatcct caccaacaca tacatcaacg gctaccgcaa
     5941 gaagcagcgg gagccaaagc agtatgcgac cgcggagctc accgatgagc agctcagcga
     6001 gatctcccgc cgggggccga cagggctgcg ctcagcagaa gagcaggcgt tggaatcgtt
     6061 gccgaacaag aagatcaagg ccgcgatgct ggcactgccc gacgagtttc ggctggcggt
     6121 gtattacgcg gatatcgagg gcttgccctg tcgagagatc gctgaaatca tgcagacccc
     6181 aggtgggacc gtcggctcgc gaatacatcg cggacgacgc cggctgcgcc atctgctggg
     6241 cagctccgcc gacacggtcg catgagactt ggccgttcac cgtctgctta cgtaggcgcc
     6301 gacccgtcgc actcaattgc tcgagctcgc cgccaccggg caccacgcgg agccggtgcc
     6361 caactgcgcg acacgattgt cagcgccgca accgagttgt tgatcgagac cggatacgcg
     6421 aaagcggttt ccattcgttc ggtcgctgag cgggtcgggg tgacctcgcc ggcgatctat
     6481 cttcacttcg cggataagga cgccttgctc gacgccgtct gcgcacagta tttccaaaag
     6541 ctcgacggac agatgcggtc cgcggccgct gggtgcacca gcacgctgga ggtgctgcac
     6601 gccctcggaa tggcctatgt ccgcttcgcg ttgaaatccc cagtgctgta ccggatcgcg
     6661 acaatggggg accctcgttc gcccagtgat gtcgacaccg tcttagccaa ctccgcgttt
     6721 gtccacctgc gcgcaatagt ccagacgatg atcgacgacg gcacctaccc gcagcgggac
     6781 gcacacaaaa tggcgcttga actgtggacg gttgtccacg gtgtcgcggc gctgctcgtc
     6841 gccaaaccgc actggctgct agatgatgtc gaggaactga ccgataaagt tcttcgcgcc
     6901 atgtgctacg gccaaatcgc tgcaggcata gtcggatacg acaagtccgc gagcgatgcg
     6961 atcgatcatc tcatgaggct caagtctgaa tcgtcgcctt tcggcgcaag taactctaga
     7021 acgcggcctc cggcaggctc atgatctcgt cgtcgaggtt ctcgacgata ccgcgcagca
     7081 cggcgagatt cggcagcacg ttcgcggcga agaacttggc cgtcgcgatc ttgccccgat
     7141 agaacccttc gtcgcccttc gttgcggcaa gcgctgtttg cgcaacgttg gcctgcacaa
     7201 gcaggcgcca tccgatgatc agatcgccga ccgcgagcag gaaccgcacc gacccgagcc
     7261 cgactttgta tatctccgcg ggctgctcgg ccgccgccat gaggtagccc gtcagcgtgg
     7321 ccgtcatcgc ctggacatgt tcgagggcgg tgcgcagctg ctcggcctgt gttttcagct
     7381 ccgcgtcgca gctgtcgatc gtatggctga tctgtgcggc aacatgtgcc agcgcctcgc
     7441 cgcggtcgcg aacgatcttg cggaagaaca ggtccagagc ctggatcgcg gtggtgccct
     7501 cgtacagcga gtcgatcttg gcgtcgcgga tgtactgctc gatcgggtag tcctgcagga
     7561 agcccgagcc gccgaaggtc tgcagcgact cggtgagcag ttcataggcg cgttcggagc
     7621 ccacgccctt gacaacaggc aacagcaggt cgtccacacg gtgcgccata gacgggtcag
     7681 cgcccgaaac atggtgagcg acatcgtcgt tctggtgcgc agcggcgtac aggtacagcg
     7741 cccgcaggcc ctcggcatag gccttctgcg tcatcaggct gcggcgcaca tccggatggt
     7801 tgatgatcgt cacgcgcggc gccgtcttgt cggtcatctg cgtcatatcg gcgccctgca
     7861 cacgctcttt cgcgaacgcc agcgcgttca ggtagccggt ggacaatgtg cctgacgatt
     7921 tgacgccgat cgtcatgcgt gcgttctcga tgaccgtgaa catctgcgcg atgccgttgt
     7981 gaacgtcgcc caccaggtat ccgacggcgg gtatgccgtg cgcgccgaac gtcaattcgc
     8041 atgtggggga ggacttcagg cccatcttgt gttcgagtcc ggtggcaaag acgccgttgc
     8101 gcgggccgag ctcgagcgta tcggggtcga acaagtacat cggcacgtag aacaggctca
     8161 gccccttggt gccgggccca gctccttcgg gtcgggccag caccaggtgg aagatgttct
     8221 cggcggtatc gccgacgtcg ccgccggaga tgaaccgctt cacaccctcg atgtgccacg
     8281 ttccgtctgg ttgagcgatg gccttggcgc ggccggcacc cacgtcggaa ccggcgtcgg
     8341 gctcggtcag caccatcgtc gccgcccagc cccgctccag gcccattgcc gcccaacgct
     8401 tctgctggtc gttgccttcg atgaacagtg cgtgagccat tgctggaccc aatgcccacc
     8461 agaagggggc cgacggattg gcgcacagca gcatctcgtg gacggcccac accagaggcg
     8521 cgggcgccgg cacgccgccg atcccctcgg cgatgccgac ccgccaccac tcggcgtcct
     8581 tgatcgcctg caccgacttg gccaattcgc cgggcacgct gatggtgtga cgctccgggt
     8641 cgaaaaccgg cgggttgcgg tcggcggcgg cgaacgactc ggcgaccgga ccctccgcca
     8701 gccgggcgac ctcctcgagc atcgtgcgcg cggtatcgac gtcgaggtcg ccgtagccgc
     8761 cggagtcgag aacagcgccg aggttcaaca cctcgaacag gttgaactcg atgtcacgga
     8821 cattggcgat gtaatgcccc aaggctgttc cctccgctca gcaaacttag gaaagtgtgg
     8881 cggagcaggt aaacgtacgc aaccgtaacc acgcgcaacc tacggtgacg gaaccgtagg
     8941 gtacgaggtc acaccatcgg gtgtgacagg catcacacct cgcagccgca gtcgggcgct
     9001 tgccctgcgg ccggcagttc ggcaagcacc tcggcacgcg ccgcgtcgaa gctgagataa
     9061 cccttgatgg gcaggcattc gggcggcgga tcctcatagc tccacgcgac atcgtcgacg
     9121 acagtgtcgc cgaccacggc ggcccagtac gtcgcgtagc ccttgtagtt gcaataggtg
     9181 ctggtgtccg tgcgccgcaa caggtcggtg cggacaagag atggatcgac atagaggcga
     9241 ggttcgagcg cggtttcgaa cacgatca
//
LOCUS       DASRJA010000708         3728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3240116,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000708 DASRJA010000000
VERSION     DASRJA010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3240116"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFC01_31935"
     CDS             complement(<1..513)
                     /locus_tag="VFC01_31935"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HZN84266.1"
                     /translation="MASESTVVNHRFELTAGTHMCCMYRGPAERDELLEPYLAEGLWA
                     DDTCFVAVGDEDPRRLRRLQTTGGPGRLIIRTAAEPAFDKDFFDIDRIIAFWEKTITD
                     ALGVEGVAFVRLSAEATWWLTQIPGQEDLLRYEARLNDFAARHPVSVLCLYDIEELGA
                     GVVFDAMKIHP"
     gene            691..2154
                     /locus_tag="VFC01_31940"
     CDS             691..2154
                     /locus_tag="VFC01_31940"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZN84267.1"
                     /translation="MKAVVYQGPRDVRVSDVPDAKIERPTDALVRITSTNICGSDLHM
                     YEGRTDFEPGRTLGHENMGEVVEVGTGVEKIRVVDWVVVPFNITCGFCENCERGLTNY
                     CLTIQPDPKAAGAANGFADMGPHDGAHEAAAPVVAGLLRLINNRRRTGQCLEAPELPA
                     AAGDRGAGERADLASSPAHPCGWMRPLESNVSGCGTDAPGATGRAPRKGVIVQVGYHS
                     ITWGGVVGHPQGVTSAKDLYYLTYGSVEQAVRDIAAAGYTGTEMFDGDLAAYADRPDE
                     LRGLLAEAGVELVSVYTGAGFVYPDVLPDELDKVRRACEFAATFGAERLVVGGGARRA
                     AGTTPEDYDRLASALDQVHEVARQHGLTASYHPHLTTVVESPEELEQLLPRTQIAFCP
                     DTAHLAAGGGDPAALIRRYGDRVAHVHLKDVALDPVEFLPLGQGSLDFRDILSAVREA
                     GYDSWLIVELDAYAGDPLEAAKISKAYLDQLLSIQVA"
     gene            2250..3362
                     /locus_tag="VFC01_31945"
     CDS             2250..3362
                     /locus_tag="VFC01_31945"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZN84268.1"
                     /translation="MATLAGCYIPGQTTTAPPDQAAAEKADQALQELEGQVLSKGPNG
                     EDPSPATAADLTPEQLDKIKTMKATAAIVMHYAGNDWATAQIAGLRSEFGNLGIEVVA
                     VTDANFKPDKQVSDLETVMSKKPNIIVSIPTDPVATATAYKAAAAGGAKLVFMDNVPK
                     GFKAGTDYVSAVSADNFGNGVVSAHLMAKALGAKGKIGLIFHEADFFVTRQRYEGFKK
                     TVQSDYPEMQIVEEQGIAGPDFAGDAQGVANAMISKNSDLVGIWAVWDVPAEGVMAAV
                     RASGRGDLRIATEDLGKNVAIALAKGELVVGLGAQLPYDQGVTEARLGALAVLGEKTP
                     PYVALSALPVQHDNVLEAWKTVYHEDPPEDLVSSFK"
     gene            3377..>3728
                     /locus_tag="VFC01_31950"
     CDS             3377..>3728
                     /locus_tag="VFC01_31950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007296998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN84269.1"
                     /translation="MTDVQAAPAPVAETDVPAVEMREISKAFDSNVVLAGVDFEVAQG
                     EVHALAGGNGAGKSTLMKILQGVYSKDSGVIRINGREVEFSSIHDAKAAGIGMVFQEF
                     SLVPSLTVAQNIYLT"
BASE COUNT          707 a         1175 c         1198 g          648 t
ORIGIN      
        1 tggatggatt ttcatcgcgt cgaagacgac gcctgcgccg agctcctcga tgtcatacag
       61 acacagaacg gacaccggat gacgtgccgc gaagtcgttt aaccgcgcct cataacgcaa
      121 caagtcctcc tggcccggaa tctgggtcag ccaccaggtc gcctctgcgc tcagccggac
      181 gaatgcgacg ccctcgaccc cgagcgcgtc ggtgatggtc ttctcccaga aggcgatgat
      241 ccgatcgatg tcgaagaagt ccttgtcgaa cgcgggttcg gccgcggtcc gtatgatcag
      301 gcgtccgggt ccgcccgtcg tctggaggcg gcggagccgc cgcgggtctt cgtcgccgac
      361 ggccacgaaa catgtgtcgt cggcccacag gccctcagcg aggtagggct ccaacagctc
      421 gtcgcgctcc gcagggccgc gatacatgca gcacatgtgg gtcccggcgg tcagttcgaa
      481 tcgatgattc accacagtgg attcgctggc catatcgcct ccgcgttcag tctgtcacca
      541 gccggacgcc gttggaaagc gcacgaaggc ccggttcgct tgtgcgccga gaccaagttg
      601 cgccgggaat ttcgacctcg cgtgccaaag acatccgccg gttcggctgg aatcgtcaac
      661 ttcgacggaa gagtccggaa ggacgtgacc atgaaggctg tcgtgtacca ggggcctcgt
      721 gacgtccgtg tcagcgatgt ccccgacgcg aagatcgaac gccccaccga cgcgctggta
      781 cgcatcacga gcaccaacat ctgtggatcc gatctgcaca tgtacgaagg gcgcaccgac
      841 ttcgagcccg gccggaccct tgggcacgag aacatgggcg aggtcgtcga ggtcggtacc
      901 ggagtggaga agatcagggt cgtcgactgg gtggtggtcc cgttcaacat cacctgcgga
      961 ttctgcgaga actgcgaacg cgggttgacg aactactgcc tgaccatcca gcccgacccc
     1021 aaagcggcgg gcgccgcgaa cgggtttgcc gacatgggac cgcacgatgg cgctcatgaa
     1081 gctgctgcgc ccgtcgtcgc cggcctgctc cgcctgatca ataatcgccg ccgcaccgga
     1141 caatgcctcg aggcacccga gctgcccgca gcggcagggg accgtggagc tggggagcgc
     1201 gcggatttgg ccagttctcc ggcacatcct tgtggctgga tgcggccgtt ggagagtaac
     1261 gtcagtgggt gtgggacgga cgccccgggg gccactggca gagctccgag gaaaggggtc
     1321 atcgtgcagg ttggctacca ttcgatcact tggggcggtg ttgtgggcca tccgcaaggc
     1381 gtcaccagtg ccaaagatct ctactacttg acctatggct cggtggaaca agccgtccgc
     1441 gatattgctg cggctggcta caccggcacc gagatgttcg acggcgatct tgccgcgtac
     1501 gccgaccgtc cggatgagtt gcgcggccta ctggccgagg ccggcgtcga actagtaagc
     1561 gtttacaccg gagccggatt cgtctatccc gatgttctgc ctgacgagtt ggacaaggta
     1621 cgtcgggcct gcgagttcgc ggccaccttc ggcgcagaac gactcgtggt tggcggcggc
     1681 gcccgacggg ctgccggcac cactcccgag gactatgacc gtctggccag cgccctggac
     1741 caggtccacg aggtcgcccg ccagcacgga ctcacggcgt cgtaccaccc gcatctcacc
     1801 acggtcgtcg aaagtcccga agagctcgag cagctgctgc ctcgtactca aatcgccttc
     1861 tgccccgata ccgctcatct ggctgccggt gggggcgatc cggcggctct catccgccgc
     1921 tacggggacc gcgtcgctca cgtacatctc aaagacgtgg cgcttgatcc cgtcgagttc
     1981 ttgccgcttg gtcagggctc gctggatttc cgcgacattc tctccgccgt ccgtgaggcc
     2041 ggctacgaca gctggctgat cgttgaactc gacgcctatg ccggcgatcc actggaggcc
     2101 gccaagatca gcaaggccta cctcgaccaa cttctctcca tccaggtcgc gtagctaggc
     2161 ggcgagctcc accagggcag acgcgatatc taacaacgga ggttctcttg agaggaacgc
     2221 tcgctcgagt cggcgtcgcc attgttgctg tggcgacctt ggccggctgt tacatcccag
     2281 gccagaccac taccgccccg cccgatcagg cagcggccga gaaggccgac caggctcttc
     2341 aggagttgga gggccaggtg ctcagcaaag gtccgaacgg tgaggatcct tccccggcta
     2401 cggcggccga cttgacacct gagcaactcg acaagatcaa gacgatgaag gcgacagccg
     2461 ccatcgtcat gcactatgcg ggcaacgatt gggcgacggc tcagatcgcc ggcctccgct
     2521 ccgagttcgg caacctcggc atcgaggtcg ttgcggtcac cgacgccaac ttcaagccgg
     2581 acaagcaggt gtccgacctg gagacggtga tgagcaagaa acccaacatc atcgtgtcga
     2641 taccgacaga cccggttgcg acggctacgg cgtataaggc ggcggcggca ggcggggcga
     2701 agctcgtgtt catggacaac gtccccaagg gattcaaagc cggcacggac tacgtctcgg
     2761 ccgtgtccgc ggacaacttt ggcaacggtg tcgtatccgc ccatctgatg gccaaggctc
     2821 tgggcgccaa gggcaagatc ggcttgatct tccacgaggc cgacttcttc gtcaccaggc
     2881 agcgctacga gggctttaag aagacggttc agagcgacta cccagagatg cagatcgttg
     2941 aggagcaagg catcgcaggc cctgacttcg ccggtgacgc gcagggtgtc gcgaacgcca
     3001 tgatcagcaa gaattccgac ctggtcggta tctgggcagt ctgggacgtg ccagctgagg
     3061 gtgtcatggc cgcggtacgg gcttctggcc gtggcgatct gcggatcgcg accgaggact
     3121 tgggtaagaa cgtcgcgatc gcgctcgcca agggcgagct ggtcgtcggg ctgggcgccc
     3181 agcttcccta cgaccagggc gtcaccgagg caaggctggg tgccttggcg gtgctcggtg
     3241 aaaaaacgcc gccctatgtc gctttgagcg cactgccggt acagcacgac aacgtcctcg
     3301 aggcctggaa gaccgtctac cacgaggacc cgcccgagga ccttgtcagc tcgttcaagt
     3361 aaggagtcag cggcaaatga cagacgtgca agctgccccg gccccggtcg ccgagaccga
     3421 tgtgcccgca gtggaaatgc gtgagatcag caaggccttc gacagcaatg ttgtcctggc
     3481 cggtgtggac ttcgaggtcg cgcaaggtga ggtgcacgct cttgcgggcg gaaacggggc
     3541 cgggaagtcg acgctgatga agatcctgca gggggtctac agcaaggatt ccggcgtgat
     3601 ccgcatcaac ggccgcgaag tggagttctc ctccatccac gacgccaagg cggccggcat
     3661 cggcatggtg ttccaggaat tcagcctggt acccagcctg accgtcgctc agaacatcta
     3721 cctcacca
//
LOCUS       DASRJA010000709        22078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_272182,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000709 DASRJA010000000
VERSION     DASRJA010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 22078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22078
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272182"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFC01_31955"
     CDS             complement(<1..285)
                     /locus_tag="VFC01_31955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZN84270.1"
                     /translation="MTAQKKAAPEFVPVVIVGAGPAGIAAATMLGQYGVECLVLDRHE
                     TVYPLPRAVHADDEIYRILARLGVADEFAAHSRQALGLRLLSPDMRVLAEL"
     gene            complement(282..1202)
                     /locus_tag="VFC01_31960"
     CDS             complement(282..1202)
                     /locus_tag="VFC01_31960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020102039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZN84271.1"
                     /translation="MTISILRTADAWWVQTTSGAARVDTDATTTAELLADRAAIDVAA
                     HSTDTVPVESLALLSPITTSCRVVAQATNYASHVKDVGRDPAKTPLTFFRKASGSVTG
                     PFGDIVKPEHVKLLDYEAEIGLVFGRELPVGTEVTDANLADFVAGLVVTNDISARDVQ
                     LPKMQFFESKSYPTFTPVGPALVLVSAEELKRFGELRLRLSVNGEVRQDMLVDGDILF
                     PPVQALQALAKFQKVDAGDILLTGTPVGTALTTHGVTPKGDIDLGAFLKAQFEKNPAY
                     LQDGDVVETTIATDDGAIDLGVQRNVVVYK"
     gene            complement(1239..2378)
                     /locus_tag="VFC01_31965"
     CDS             complement(1239..2378)
                     /locus_tag="VFC01_31965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016895408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN84272.1"
                     /translation="MSEDVIGAHNGLHSEQGALPGEHRGRSANPIIKVHDVHWLEFEK
                     PDLVKAEAFAHSFGFATALRTKDELHLRGSDAGAPCVLIRRGQREKFLGPAFVAAEQS
                     DLVRLADATGSTVRPLPDTLGGVTVDLVDPSGLPVRVVSGTHQLEALTTAQTPHVFNF
                     GHEKVRTNAMQRPPRVPTNVQRLGHIVVQTPKYIEALNWYLDTFGMIVSDFSYYPGQR
                     ERGPILSFVRCDRGSVPADHHTLAMALGPVKRYMHSAYEVADLDALAAGGEYLREQGY
                     FRSWGIGRHIEGSQIFDYWRDPDGFLVEHYADGDMFDNTLEPGWAAMHASGLYQWGPP
                     PSKDFLGLGSARETAAELALMVDALRHDNEFDLSALRGLFAVGNS"
     gene            complement(2913..4166)
                     /locus_tag="VFC01_31970"
     CDS             complement(2913..4166)
                     /locus_tag="VFC01_31970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HZN84273.1"
                     /translation="MATILAYTSPALGHLFPFSALLEELARRGHEIHLRTLSTGVQLG
                     RSLGFTTEAIDPRIEALVHEDWKASNPRAALKMAVDMFCRRAAYEVPDFTDAITRTRP
                     DALLVDINTWGAMSVADAGDIPWACFSPYTPPLQSHGVPTFGLGLAPLTGVLGRVRDA
                     ALRPIVMGMIERAVLPHGNRIRASAGAPPIASADEFMRRAPLMLVASGKPFEYPQTDW
                     GDAVQMIGPCMYEPAMDSVPDWLEAIDRPIVLVTTSSQQQADMKLVVTAMTALADQPV
                     HVVATLPAGLPSDVTITPNATVLEFVPHGPVLDRAVCAVTHGGMGATQKALARGVPVC
                     VVPFGRDQFEVARRVEVAQCGTRLPAKKLTARRLSAAIRQAMTMTDGARRVAAGFVAT
                     GGVPRGVDLFEQRVLTCSSSADKKA"
     gene            4416..4682
                     /locus_tag="VFC01_31975"
     CDS             4416..4682
                     /locus_tag="VFC01_31975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZN84274.1"
                     /translation="MSTENSFTVAREAVTQHIIYLVGKDGEIATLTADSSLQSQGLDS
                     LKVMSLVFKIEEHYDIVLDEGDADDLRTVDDLAALVVRRIEEQS"
     gene            4679..6460
                     /locus_tag="VFC01_31980"
     CDS             4679..6460
                     /locus_tag="VFC01_31980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HZN84275.1"
                     /translation="MRGAPAFRPKYSTLIEMLAHAARSDVSLFFVDRKEQDLEVPMAR
                     VRQRAMSIAADLMARGVKKGDRVALVLPTCPEFVECLFGVICAGAIPVPLYPPVRLGK
                     LGEYHSKTAAMLRAVDAALVVTDERIRRLLGTSVQLAAPRLGCVTASSLDGENSVEIG
                     ATADTVALIQFSSGTTQDPKPVALTHANLLSNLASIEHYLIADGAEQPVGVTWLPLYH
                     DMGLIGNLLSAFYMQCRMALLPPELFVAEPGAWLRAISRHRGTVTAAPNFAFGLCLKR
                     IRDEELDGVDLSSWTVSLNGAEAVSAAVQQRFIERFTRWGLRPSALTPCYGMAEASLG
                     VTFKPRGTLFRTLGVDADKLAREGVVAPGSKELVSVGPPLAGVEVEIRDSAGIPLPPG
                     QVGRIFVRGASVMAGYFGRTDLTDQAIHDGWLDTGDLGFTQDGELFVCGRHKDIIVVR
                     GANHAPEEFEAALESLPGVRTGCAVAVGFVPAGHDDEALAMLVETTSDGPSTLADDVA
                     SRVQERTQIRPAHVEVLAPGTLPRTSSGKLRRREARTQWLTGTLLPPKKVSSARILAA
                     AAHGELSHARARFARRAANTPPVITKR"
     gene            6553..7608
                     /locus_tag="VFC01_31985"
     CDS             6553..7608
                     /locus_tag="VFC01_31985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HZN84276.1"
                     /translation="MRPTLLGIASAHPPFVVTAQDSWELLFQHMSPPIRFGKRIVEST
                     GVQKRHIIWDPDALRDACGLQTGDRMAAHAEAVMDVASRSVGQAIEGFDKRRIGSFVM
                     ACSTGYVNPGPDLLIAKQLELRSDLRRTFIGHMGCYAALNVIKVAMDSMAARPDELVI
                     CNCTELSSAHVRDAPMPDEDPMESLITQALFGDASVTMLMGSAPDGAGIQFLRTHTEQ
                     LYDQDQMMSLNIGNQSFWMTLAAGVPAALQDNIEGFLVKLLQPLGLSASDVSHWGIHP
                     GGPKILRVLGKQLALQPAQLRASWDVLANAGNCASATVLLVLENILRVDKPRRGEYGV
                     LLTFGPGLTIEGAVIRF"
     gene            7691..9952
                     /locus_tag="VFC01_31990"
     CDS             7691..9952
                     /locus_tag="VFC01_31990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN84277.1"
                     /translation="MLENIARLAIRAPRRIIAVALLVMVGAAVFGIPVANDLSPGGFD
                     DPTAGSAQAAHLLADTFHQGDTQMLITVSSGGGAQGGAARAVGTDIVAQLHNSPYVTQ
                     VVSPWTAPPSAAKSLVSKDGNTGLIVAGITGGEKGAQQHADTLSTEVVHDRNGVTVRA
                     GGDAIAGTQITHQTQNDLKLMESIAIPLSFVVLVWVFGGVVAAALPLAVGGFAIFGSM
                     AAMRALTFATDISIFALNLSIAMGLALAIDYTLLILSRFRDELADGADRDEALRRTMA
                     TAGRTVLFSSMTVALSMSVMALFPMYFLKSFAYAGVAVVALAALAAIVVTPAAIALLG
                     PRLDSFNVHRLLRRLFRRPEPLRRPVERTFFYRLAKFAMRQAIPILLAGAALLIALGL
                     PFSQAKWGLPDDRVLPESSSARQVGDQLRTDFAENLGTNVTIVIRDASDISTAEADRY
                     ASQLSQVNAVSSVSSPAGTFVAGARVGPPSAPTGVSDGSEFLTVTSSAPMYSEASQTQ
                     LDGLAAVPGPAGRHALLTGTAAINHDTARAVSSRLPIVLTVIAAITFVLMFRLTGSVV
                     LPLKTLLLNGLSLTATFGALVWIFQDGHLGALGTTATGTLVATMPALLFCIAFGLSMD
                     YEVFLVSRIHEYWLASDQSPAANDESVAQGVAHTGRVITAAALIMSISFAAMIAAQVS
                     FMRMFGLGLTLAILGDATFVRMLLVPAFMRVMGEFNWWAPKPLARLHERFPITSEFTT
                     PSAPPAEGLSYSR"
     gene            9964..10680
                     /locus_tag="VFC01_31995"
     CDS             9964..10680
                     /locus_tag="VFC01_31995"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HZN84278.1"
                     /translation="MWSAVFFLSLVVAMDPVRVGITTLLLSRPRPVVNLFVFWLGGIT
                     AGIAVSLVVLLFLRDLALPVLRVAISAISSPTAAHIQVVIGILAVLIAARFWARRRVP
                     AAVTGADAAGPLLHPNTVTAPGRLSLRERLEGGSLAMVFVAGLALATPPVEYLAVLIT
                     ILASRATVAAQLGAALMFTLVAFTVVEVPLIGYLTTPAKTLDVVRRLNEWIHSRRQVI
                     PCVLIGVVGAVLMATGMGKV"
     gene            complement(10742..11311)
                     /locus_tag="VFC01_32000"
     CDS             complement(10742..11311)
                     /locus_tag="VFC01_32000"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN84279.1"
                     /translation="MATDNNRGLRSDASRNYSRILDAAAQAFEQEGPTVSLDEIAARA
                     GVGVATLYRRFRTREELIAAVAEHVFAADVATAIVEGGPDPWTDLVATLSATVDAFAA
                     HPVLVGLARESAVFDLSAKSEYLAAIERVLNRARDAGVIRPELTVDDVTAIVVMTLTM
                     LRRGGRGNGRRYLALLVDGMRPVGHRLPR"
     gene            complement(11338..11529)
                     /locus_tag="VFC01_32005"
     CDS             complement(11338..11529)
                     /locus_tag="VFC01_32005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HZN84280.1"
                     /translation="MKVFADRDVCIGAGMCVMTAAMFDQDEDGIVMVDAEEVPAEEQR
                     RVRNAISLCPSGALQLIAD"
     gene            complement(11537..12733)
                     /locus_tag="VFC01_32010"
     CDS             complement(11537..12733)
                     /locus_tag="VFC01_32010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN84281.1"
                     /translation="MSQTRAIPPYLMRDGVEPSAGLRGIRESAAVHATFTAFGEPAYL
                     VTRYADIKAALADHERFSNRRTTTFGGLTAEQNGIDAHDPPGALLATDPPQHHRLRRM
                     LAPMFTIRQMNHLESRIVEIVDEHLDAMEEAGAPADLVASFALPVPSLVICELLGVPY
                     ADRDGFQRRSLRQLDMALAPVERQTVADESRSYMRSLVERARRAPGDGLIGTLVREHG
                     DEVSDDELAGIGALLLVAGHETTSNMLALGTVVLLQHPAQLAAVRDDPAAVGPAVEEL
                     LRYASVVQTAIPRIATTDVEIGGVSIPGGSLVLLSLPSANHDPRFIETADSLDIRRGA
                     PGHLAFGHGVHHCLGAPLARMEMRIALPALLRRFRGLKLAEDLTEISFKPTSLVYGAR
                     SLSVTW"
     gene            complement(12913..13140)
                     /locus_tag="VFC01_32015"
     CDS             complement(12913..13140)
                     /locus_tag="VFC01_32015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84282.1"
                     /translation="MKTLTLAIATAVAAASAGLVSLATTPAAMNQADCPYGWYWDDYR
                     QNCLPPGLPKDPPNGCLTGSGTHANGTICIN"
     gene            13635..15023
                     /locus_tag="VFC01_32020"
     CDS             13635..15023
                     /locus_tag="VFC01_32020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZN84283.1"
                     /translation="MAYQTVNPYDNQIVATFDDLTDEQAMSLLTQAQKTFESWSQTSF
                     PERTAVIKRAAAIFRERSDEFARLLTLEMGKLYAEALGEVELSAQILDYYAENAEAFL
                     APEPLDTASSDDNAMLVSAPLGVILGVQPWNFPYYQLARVAGPNLMAGNVVIVKHASN
                     VPQAAAAFEKLLLEAGAPKGAYTNVYATKEQINLLIDDPRVRGVALTGSEGAGAVVAA
                     RAGKNLKKSTLELGGSDAFIALADCDLDKTVEWALHGRVFNGGQCCVASKRIIVVDEI
                     ADEFLAKFQEAMSELKAGDPFDEATTLAPMSSQRAADDLYGQINAAVEAGAKAISCGD
                     LVPTTGAFVQPTILTDITPDNPAYYQEFFGPVALFFRVKDEDEAVRLANDSRFGLGGS
                     VFTSDVARGIEIAKRIDTGMVNINHPARLKADLPFGGVKISGYGHELAHLGIEEFINK
                     KLINVVPIDAPA"
     gene            15059..16759
                     /locus_tag="VFC01_32025"
     CDS             15059..16759
                     /locus_tag="VFC01_32025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZN84284.1"
                     /translation="MPNFTVGKYLAARLEQIGLKHYFMVPGDYNLVLLDELLENENIE
                     QIGCCNELNAAYAAEGYARVNGAGAVLTTFNVGAFSALNGVAGAYAERLPVIVVSSGP
                     NTNDVGSNHVLHHTMDNGDLSDQYEIFRKVTCKAVRIVHPDTAPALIDEAISAALRER
                     KPAYIEIPCNLSAAPCSEPAPFDTLPAWEPSNARMLTDAVGRASDLVQQAKKPILLAG
                     VHLRAHGAIDAFRELAEALGCAVAVMPNAKGFFPEDHPQYVGIYWGEVSSPQCQAIVD
                     WADLVVAAGAVFNDYTTVGWSAQPARGRLIGAEPRKVQLPDAEYHGVALAEFLSDLAK
                     KVPANAATLTQYHRLHRTTSAQVAVADVDPTAPLSRVEMWRQIEQDLDEKSTLLVETG
                     DSWLNGMYTRLPGGARFEIEMQYGSIGWSVPATFGYAKGLEPDRRLVSVIGDGSFQLT
                     AQEVSNMIRHGQELMIFLVNNRGYVIESAIHDGPYNYYKNWDYAGLIDVFNAGEGAGL
                     GLVASTAGELAEAISKAHGHKGGPVLIECRIPNDDASPELISWGSKVASVNGRPDPRF
                     "
     gene            complement(16911..17798)
                     /locus_tag="VFC01_32030"
     CDS             complement(16911..17798)
                     /locus_tag="VFC01_32030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN84285.1"
                     /translation="MVRLGSTESPRAEQVCAARTQSGPRALQDLLDAARAAVQTVRRA
                     AAVQANEAAMMMSTVAMAPLRMLGGSYDTASSSLTVKALTAPTTRPVLLVHGFAGTKS
                     SWALVANALKARGVAVEAMSYPPVGTSVQQLAERLVAEVERILVATGTDKVHLVGHSL
                     GGVVIAQAIADSRLTGRVDTVITLGSPFGGSPWAGVLPFLEIVRELRAGSPVLNRLAS
                     APLPDGIRWLSLTAALDIIVPGVRAVPSHAQAETITFEGVGHLGMLVSRQVIACIAAE
                     LCTRRTVAEERMPVLPNVS"
     gene            18678..19658
                     /locus_tag="VFC01_32035"
     CDS             18678..19658
                     /locus_tag="VFC01_32035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN84286.1"
                     /translation="MKAAVVRALGQPPEYADFPDPKARDGAIVATVEAAALKNLDRAL
                     VSGTHYNSARLPLPGIAGVDGVARLDDGRLVYANALPAQGMMAEKTLINPASAVELPE
                     GIDPVVAAAVPNPALSAWLSFEHRAHMREGQHVLVLGATGVTGSLAVQLAKAAFGAGR
                     VVAAGRNAARLEWLQTVGADEVIPLETEDLATCIAAQHRMQPFDVVLDYLWGPPAEQA
                     LAALGNVDPNATSHVTRFVQIGDMAGSSITLPAGILRSAGIELVGMGFGSVPAAAWAR
                     AAADHLPRLFDMVADGRLHLQTERRPLSQVEDVWTQKEPSGTRVVFVPGQ"
     gene            complement(19679..20866)
                     /locus_tag="VFC01_32040"
     CDS             complement(19679..20866)
                     /locus_tag="VFC01_32040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020111758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN84287.1"
                     /translation="MRRPADVPVYEPDLYATQAIVDPHPHYARLRRLGPVVWLAKQRV
                     YALPRYAECKAVLRDDTTFISGEGVGLNRVFNRLSRGTTLNSDGDEHDQRRKLVAHRL
                     LPRALRTMSDVVTSQAEQLVAEAVRRQHVDGVTDLATALPLSVVPDLVGWPEDRRHHL
                     LRWGGATFDVLGPLNRFSLKSVPASMQMLRFSKNVVRRRSVIRGSMGHDVLTAADAGN
                     LRHSECSALMIDYLVPSLDTTISGIASALALFASHPEQWDMLRAEPSLLPNVINEVLR
                     HESPLRAFSRKSLQPTEIAGVGIPAGSRFLVLYASANRDELEWTEPDAFDIRRNATRH
                     LGFGHGTHACAGQGLARLEMQAMLAALMQQVSRIELAGEPTWTINNIIRCYEHLPLRL
                     IPA"
     gene            complement(20859..>22078)
                     /locus_tag="VFC01_32045"
     CDS             complement(20859..>22078)
                     /locus_tag="VFC01_32045"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HZN84288.1"
                     /translation="PHVPVSQLQATRGSGDRPELIRNAEVMEAVQIAPDRVQVAFTDR
                     VSGEEHRVETRYVLGCDGANSLVRAAIGASMEDLKFQQRWLVVDVATTAELNQGESVH
                     QVCDPHRAATYMRIGDTRYRWEFRLLPDEVADDYSTTAALYPLIQPWVEGVAPEQLQL
                     VRVAEYTFRAQVANRWRRGNMFILGDAAHLTPPFIGQGMGAGLRDATNLAWKLAGAVN
                     GWLPESALDTYEQERIPHARYMIRYALAVGAAMTAGGEVGNFLRRAVVPRLHLIPGVR
                     KKIADSATPALHSSELVVKSSGRRELAGRLCPNPVLVDGKRFDEVVGARFAVVTSAPL
                     DPSQQDELTRRGAVVVTAHPGTELDLWLRAGRATAAVVRPDRTVLCASRNLSEVCNAV
                     PGFASAADTRADA"
BASE COUNT         3969 a         7005 c         7061 g         4043 t
ORIGIN      
        1 gagctcggcc agcacccgca tgtccgggct gagcagccgc agccccagcg cctgccggga
       61 gtgcgccgcg aattcgtcgg cgacacccaa ccttgccagg atgcgataga tctcgtcgtc
      121 ggcgtgcacc gcacgcggaa gcggatagac cgtttcgtgg cggtccagca caaggcattc
      181 cacgccgtac tggcccaaca tcgttgcggc ggctatgccg gcggggccgg cgccgacgat
      241 gactactggc acgaactccg gcgcggcctt cttctgtgcg gtcatttgta caccaccacg
      301 ttgcgctgga cgccgaggtc gatggcgccg tcgtcggtgg cgatggtggt ctcgaccacg
      361 tcgccgtctt gcagatacgc gggatttttc tcgaactgtg ccttcaggaa tgcgcccagg
      421 tcgatgtcgc ccttcggggt aacgccgtgt gtggtaagcg cggtgccgac cggggtgccg
      481 gtcagcagaa tgtcgccggc atcgaccttc tggaacttcg ccagcgcctg cagtgcctgc
      541 accggcggga agaggatgtc accgtcgacc agcatgtcct gccgcacttc accattcacc
      601 gacagccgca ggcgcaactc accgaaacgc ttgagctcct ctgcggagac cagcaccagt
      661 gcaggaccga ccggcgtgaa cgtgggatag gacttcgact cgaagaactg cattttgggc
      721 agctgcacat cgcgcgccga gatgtcattg gtgaccacca agccggccac gaaatcggcc
      781 aggttggcgt cggtgacctc ggtgcccacg ggcagctcgc gaccgaacac cagaccgatc
      841 tcggcctcgt agtccagcag cttgacgtgc tctggcttca cgatgtcacc gaacgggccg
      901 gtgaccgagc ctgacgcctt gcggaagaag gtcaggggcg tcttggcggg gtcgcgaccg
      961 acgtccttga cgtgcgaggc gtagttggtg gcctgagcga ccacgcggca cgacgtcgtg
     1021 atgggagaca gcaacgcaag gctctccacc ggcacggtgt cggtgctgtg cgcggcaacg
     1081 tcgatggccg cacgatcagc cagaagttcg gcggttgtgg tcgcgtcggt gtcgacgcga
     1141 gccgccccgg acgtggtttg cacccaccac gcgtcggcgg tgcgcaggat cgagatggtc
     1201 ataatggcgt tcttctttct ttcggttggg tattcggatc aggaattgcc tacggcgaac
     1261 aaaccgcgta gggcggacaa atcgaattcg ttgtcgtggc gtaatgcatc gaccatcagg
     1321 gcaagttcag ctgcagtctc gcgcgctgat ccgaggccca ggaagtcctt gctcggcggc
     1381 ggtccccact gatacaggcc ggaggcatgc attgcggccc aaccgggctc cagggtgttg
     1441 tcgaacatgt caccgtcggc atagtgctcg accaggaaac cgtccgggtc acgccagtag
     1501 tcgaagatct ggctgccttc gatgtgacgg ccgatgcccc acgagcggaa atacccctgc
     1561 tcgcgcagat attcgccgcc ggcggccagt gcgtcgagat ctgcgacctc gtacgcggaa
     1621 tgcatgtagc gcttgaccgg acccagcgcc atggccaggg tgtggtgatc ggcggggacg
     1681 gagccgcgat cgcagcggac gaagctcagg atcgggccgc gctcgcgctg gccggggtag
     1741 taggagaagt cgctgacgat catgccgaac gtgtcgaggt accagttgag ggcctcgatg
     1801 tacttcgggg tctgcaccac gatgtgtccg agccgctgca cattggtggg cacccgcggc
     1861 gggcgctgca tcgcgttggt ccggaccttc tcgtggccga agttgaacac gtgcggcgtt
     1921 tgcgcggtgg tcagcgcctc gagctgatgg gtgcccgaca ccacgcgcac cggcagcccg
     1981 ctcgggtcga ccagatcaac ggtcacgccg cccagggtgt ccggcagcgg gcgcacggta
     2041 gacccggtgg cgtcagccag ccgcaccaga tcggactgct cggcggccac gaaggccggc
     2101 ccgaggaact tctcgcgctg accacggcgg atcagcacac acggcgcacc ggcgtcgcta
     2161 ccgcgcaggt gcagctcgtc ctttgtgcgc agcgccgtgg cgaaaccaaa ggagtgggca
     2221 aacgcctcgg ccttgaccag gtcaggcttc tcgaactcca gccagtgaac gtcgtgcacc
     2281 ttgatgatcg ggttggccga gcgcccccga tgctcgcctg ggagcgcgcc ctgctcgctg
     2341 tgcaagccgt tgtgcgcgcc gatgacatct tcactcatcg ctgacctcct aagactatac
     2401 cgatgaacgg catgatgctc ctttactgaa atagcctttc tgcaaaggca tttcgctatg
     2461 acgcggtact cgcgtatcgc attgcaccaa gcatgcccgc catagaaaga catgtcgttg
     2521 gggctgctcc cagttgatgc acccggctag ccggtattca cgatctttct gacgattcgt
     2581 cactaatgcg ctgctcttca atgcgtttcg tatgcgatac gacgatcaac ccacgaacgt
     2641 ccctcggcat cgccacgctc ccggccccta tgcagcttcg gcgctagtcg caccagcctt
     2701 cgggggttag gtccaacgac ttctaggcac aagcacgcga cgctaatggc tacaggggca
     2761 cctggatcac gcacagtagg cggcgcatct gggggcgcct ttcaccgcgt acacatgcgc
     2821 gacgacgata tgagaggcct actaccccgc taacgcgtat tgccgtcttc tgacgaagtc
     2881 tcagtaatgt agtgcgtcac aacctcttcc ggtcatgctt tcttatccgc tgatgagctg
     2941 caggtcaaca cccgctgctc gaagaggtca accccacggg gcacgccgcc ggtcgccaca
     3001 aatcccgccg cgacccgacg tgcgccgtca gtcatggtca tcgcctgtcg aattgcagcc
     3061 gacagccgcc gtgcggtcag cttcttcgcc ggcaggcgag tgccacactg ggctacctcc
     3121 acgcgccggg cgacctcgaa ctgatcacgc ccaaatggca ccacgcatac cgggacgcct
     3181 cgggcaagag ccttctgagt cgcgcccata ccgccatggg ttaccgcaca caccgcccga
     3241 tccaaaacgg gtccgtgcgg tacgaattcg aggaccgttg cgttcggtgt gattgtgacg
     3301 tcgcttggta gcccggcagg tagcgtcgcg accacgtgta ccggctggtc cgcgagtgcg
     3361 gtcatggctg tgaccacgag cttcatatcg gcttgttgct gcgatgatgt cgtgaccaga
     3421 acgatcgggc ggtcaatcgc ttccagccag tcgggcacag aatccatggc cggttcatac
     3481 atgcaggggc cgatcatttg gacggcatcg ccccagtccg tctgtggata ctcgaacggc
     3541 ttcccgcttg ccaccagcat cagtggagcc cgccgcatga actcatccgc cgatgcgatc
     3601 ggcggtgctc ctgcgcttgc gcggattctg ttgccgtggg gcaagaccgc cctctcgatc
     3661 atgcccatca cgatcggtcg cagcgccgca tcgcggaccc ggcccagcac gccggtcaat
     3721 ggagccagac ccagcccgaa ggtgggcacg ccatgagatt gcagaggcgg cgtgtaggga
     3781 gagaaacacg cccacgggat gtcaccggcg tcggcgaccg acatagcacc ccaagtattg
     3841 atgtcgacga gcaacgcgtc ggggcgcgtt cgcgtgatcg cgtcggtgaa gtcgggtact
     3901 tcgtaggcag cgcggcggca gaacatgtcc accgccatct tcaatgcagc gcgcggattt
     3961 gaagccttcc agtcttcatg aaccagcgcc tcgattcgcg gatcgatcgc ctcggtggtg
     4021 aacccgagac tacggcccag ctgcacaccc gtggacaggg tgcgtagatg gatctcgtgg
     4081 ccgcggcgtg ccaattcctc gaggagcgcg ctgaacggga acaggtgtcc cagcgccggg
     4141 gatgtgtaag ccaagatagt tgccatcggt accacgtctt ctcggtgggt gtgagcatcg
     4201 tgcgtctcac attgtcacga gtgagcagtg ccgcgtcggc accgctggcg cccatcgatg
     4261 tttggtgcca gcaccacccc tcagccgcgc gagtcgcgaa accggctgta ccgcagcagc
     4321 actacgtatt gcggccggcg gctggtgctg gcccccaatg catatggttg ccagcggtgc
     4381 cgacatggaa ggcctcaccc gcgacactga tgaccatgtc gacagagaac tctttcactg
     4441 tagcgcgtga ggctgttacc cagcacatca tctacctcgt cggtaaagac ggcgaaatag
     4501 ccactctcac agccgattcc tccctgcaga gccagggtct ggattcgctg aaggtcatgt
     4561 cgctcgtctt caagatcgaa gagcactacg acatcgtcct ggacgagggc gacgcagatg
     4621 atctgcggac cgtcgatgat cttgcggctc tagtcgtccg acgcattgag gagcaatcat
     4681 gagaggcgcg cccgcgttcc gtccgaagta ctcgactctg atcgagatgc tcgcccacgc
     4741 ggcgcgctcc gacgtgagcc tgttcttcgt agaccgaaaa gaacaggatc tcgaagtccc
     4801 aatggctcgc gtgcgtcagc gcgcgatgtc gattgctgcg gacctgatgg ctcggggcgt
     4861 aaagaagggt gatcgcgtcg cgctcgtgct gccgacctgc ccggagttcg tcgagtgctt
     4921 gttcggcgtg atatgcgcgg gagccattcc ggtgccgctg tatccgcctg ttcggctcgg
     4981 caaactcggt gagtatcact cgaagacggc cgcgatgctt cgggcggtgg atgccgccct
     5041 ggttgtgact gacgaacgca tccgccggct gcttggcacc tcggtccagc tggccgctcc
     5101 tcgcctgggc tgtgtgaccg catccagcct tgatggcgag aactcggtcg agataggcgc
     5161 cactgcggac acggtagcac tgatccagtt ctcctcgggc acaacgcaag accccaaacc
     5221 tgtcgcactc acgcatgcga acctgctgtc gaaccttgcc tccatagagc actatctcat
     5281 cgcggatggc gccgagcagc ctgtgggcgt cacctggctg ccgctttacc acgacatggg
     5341 attgatcggc aatctgcttt ctgcgttcta catgcagtgc cgaatggccc tattgccgcc
     5401 ggaactgttc gtcgcagaac ccggcgcgtg gctccgggcc atctcacgtc atcgagggac
     5461 ggtcacggcg gcacccaatt tcgcgttcgg tctgtgcctc aaacgcattc gcgacgagga
     5521 actcgacggt gtcgacctgt cctcgtggac ggtctcgctc aacggagcag aagcggtcag
     5581 cgccgcggtg cagcagcgat tcattgaacg gttcacacgc tggggcttgc gcccatcggc
     5641 attgacaccg tgctacggga tggcagaagc ctcgctgggc gtgacgttca agccaagggg
     5701 cacgttgttc cgcaccctcg gcgtggacgc cgacaagcta gcgcgcgaag gcgtcgtcgc
     5761 ccccggctcc aaggagctgg tgagcgtggg ccccccgctg gccggcgtgg aggtggagat
     5821 ccgcgactcc gctggaatac cgttgcctcc cggccaggtt ggccgcatct tcgttcgcgg
     5881 ggcgagcgtc atggccggct acttcggccg gaccgatctg accgatcaag caatccacga
     5941 cggctggctg gatacgggcg atctcggttt cactcaggac ggcgagctgt ttgtctgcgg
     6001 tcgtcacaaa gacatcatcg tcgtgcgcgg ggccaaccac gcgcccgaag agttcgaggc
     6061 agccttggag tcactgccgg gcgttcgcac cggatgcgct gtcgctgtgg gattcgttcc
     6121 cgcggggcac gatgacgagg ctctggccat gcttgtcgag accacgtcgg acggcccgtc
     6181 gactctggcc gacgatgtgg cgtcacgagt ccaggaacgc acacagatcc ggcccgcaca
     6241 tgtggaggtc ctggcacctg gcacgttgcc gcgtaccagc agcggaaagt tgcgacggcg
     6301 cgaggcccgt acccagtggc tgacaggcac gctgttgccg ccgaagaagg tctcatccgc
     6361 aaggattttg gccgcggcgg cccacggtga gctttcccac gcgcgcgcga ggttcgcgcg
     6421 gcgggcggcc aacacgccgc cggttatcac gaagcgatag ggagccaggc catgtacgtt
     6481 gcggacacca ccattgacat accctctggg ggtgcgccct caccggcgca tcagacggct
     6541 aacgccgttc gcatgcggcc caccctgctg ggcatcgcct cggcgcatcc gccgttcgtg
     6601 gtgacggcgc aggacagctg ggagcttttg tttcagcaca tgtcgccacc aattcggttc
     6661 ggaaagcgca tcgtcgagtc aaccggggtc caaaagcgtc acatcatctg ggatccggat
     6721 gctctgaggg acgcctgtgg cctgcaaacc ggtgaccgga tggccgccca tgccgaagcc
     6781 gtcatggacg tggcgtctcg ttcggtcggc caagcgatcg aggggttcga caaacgccgc
     6841 atcggaagct tcgtgatggc atgttcgacc ggttacgtca accccgggcc cgacctcctg
     6901 atagcgaaac agcttgagct gcggtccgat ttgcggcgga cgttcatcgg ccatatgggt
     6961 tgctatgcag cgctcaacgt catcaaggtc gccatggaca gcatggccgc acggcccgac
     7021 gagctggtga tctgcaactg caccgaactg agttccgccc atgttcgcga tgctccgatg
     7081 cccgacgagg acccgatgga atcgctgatc acgcaggcgc tgttcggcga tgcaagcgtg
     7141 accatgctga tgggatccgc accggacgga gccgggatcc agttcctgcg cacgcacacc
     7201 gaacagctct acgaccagga ccagatgatg agcctgaaca tcggcaacca gtcattctgg
     7261 atgacgctgg ccgccggcgt accggccgcg ctccaagaca acatcgaagg atttctcgtc
     7321 aaactcctgc agccgctagg actgtcggcc agcgatgtaa gccactgggg aatccaccca
     7381 ggcggtccga agattctgcg ggtgctggga aaacagttgg cgttgcagcc ggcgcaattg
     7441 cgggcaagct gggacgtgct tgcgaacgcc ggaaactgcg cctcagccac agtgctcctg
     7501 gtactcgaaa atatccttcg agtcgataag ccgcgccgcg gagagtacgg cgtgctgttg
     7561 accttcggac cgggactcac catcgagggc gccgtaattc ggttctgaat cagatcactc
     7621 gcgtcgcagc ccatcgtagg aagcgctgat cgtgggaaag cacaccaaag ggatttgggg
     7681 tagaggactt gtgttagaga atattgctcg gctggccatt cgagcgccgc ggcggatcat
     7741 cgccgtcgcg ctgctcgtca tggtcggcgc ggcggtgttc ggcatacccg tggcgaatga
     7801 cttgtcgcca gggggttttg acgatccgac cgcaggttcc gcacaggccg ctcatctatt
     7861 ggccgatacg ttccatcagg gcgacaccca gatgctgata accgtcagct ccggcggcgg
     7921 tgcgcaaggc ggtgccgctc gcgccgtcgg taccgacatc gtggcgcagc tgcacaactc
     7981 cccctatgtg acacaggtgg tctcgccctg gactgcaccg ccgtccgcgg cgaagtcgtt
     8041 ggtcagcaag gacggcaaca ccgggctgat cgtcgcgggt atcaccggcg gtgagaaggg
     8101 tgcacagcag catgccgaca ccctcagcac cgaagtggtc cacgaccgca acggcgtgac
     8161 ggtgcgcgcc ggcggcgacg ccatagccgg tacccagatc acccaccaga cccaaaacga
     8221 cctcaaactc atggaatcca tcgcgatccc attgagcttt gtggtgctgg tgtgggtgtt
     8281 cggcggtgtc gtggcggcgg cgctgccctt ggcggttggc ggcttcgcga ttttcggttc
     8341 tatggcggcg atgcgcgcgc ttacgttcgc gacggacatc tcgatcttcg cgctgaatct
     8401 gtccatcgcg atggggctgg cgctggcgat cgactacacg ctgctgattc tcagccgatt
     8461 ccgcgatgag ctcgccgacg gcgcagaccg cgacgaggct ctgcggcgca ccatggccac
     8521 cgccgggcgg actgtgctgt tctcatcgat gacggtggcg ttgtcgatgt cggtgatggc
     8581 gctgttcccg atgtactttc tgaagtcatt cgcgtatgcc ggcgtggcgg tggtagctct
     8641 cgcggctctc gcagcgatcg tggtgactcc ggcggccatt gcactgcttg gcccacgatt
     8701 ggattcgttc aatgtgcacc ggctgctgcg tcgacttttt cggcggccgg aacccctccg
     8761 gcgtccggtt gagaggacct tcttttatcg gttagcgaaa ttcgctatgc gccaggcgat
     8821 cccgatcctt ctcgccggtg ccgcactgct gatcgcactg ggtttgccgt tctctcaagc
     8881 gaagtggggt ctgcccgacg atcgcgtatt gccggaatcg agctcggcgc gccaggtcgg
     8941 cgatcagctc cgtaccgatt tcgcggagaa cctgggtacg aacgtcacga tcgtcatccg
     9001 cgacgcttcc gatatcagca ccgcggaagc ggaccggtac gcctcgcagc tctcgcaggt
     9061 gaacgcggtc tcatcagtct cgtcgccggc tggcacattt gtcgcaggcg cacgcgtggg
     9121 accgccctcg gcgccgaccg gggtcagcga cggcagtgaa ttcttgaccg tcacaagcag
     9181 cgcgccgatg tactctgagg cctcgcagac gcagctcgac gggttggctg ctgtgcctgg
     9241 tccggcaggt aggcacgcgc ttctgaccgg gactgcagcg atcaaccatg acaccgcacg
     9301 tgccgtcagc tctcggctgc ccatagtcct cacggtcatt gccgcgatca cgtttgtgct
     9361 gatgttccga ctcaccggca gtgtggtgtt gccgctgaaa accttgctgc tcaacggctt
     9421 gtcgctgacg gcaacgttcg gcgcactggt gtggatattc caggacggtc atctgggcgc
     9481 gttgggtacc accgccaccg gcacgctggt ggccaccatg cccgcgctgt tgttctgtat
     9541 cgcgttcggc ctgtcgatgg actatgaggt gttcctggtg tcccggatcc atgaatactg
     9601 gctggcgtcc gaccagagtc cggccgccaa cgacgagagt gtggctcaag gggtcgccca
     9661 cacgggccgg gtgatcactg cggccgcatt gatcatgtcg atctcgttcg cggcaatgat
     9721 cgctgcccag gtgtcgttca tgcggatgtt cggtttgggg ttgacattgg cgattctggg
     9781 agacgcaacg ttcgtgcgca tgttgctcgt cccggcgttc atgcgtgtga tgggcgagtt
     9841 caactggtgg gcacccaaac cgcttgcgcg gctgcacgag cggtttccga tcactagtga
     9901 attcacaacg ccatcagcgc cgccggccga gggtttgtcc tactcccgct gagggtgacg
     9961 aatatgtgga gcgccgtctt cttcctgtca cttgttgtgg cgatggaccc ggttcgcgtc
    10021 gggatcacga cgctcttgct ttcccggcca cgtcccgtag tcaatttgtt cgtcttctgg
    10081 ctcggcggca tcacggccgg catcgcggtc tcgttggttg tgctgctgtt tctacgcgat
    10141 ctcgcgcttc ccgtactgcg agttgcgatt tccgccataa gcagtccgac cgcggcgcac
    10201 atacaggtgg tcatcgggat tctcgccgtg ctgatagcgg cgcgcttctg ggcacgcagg
    10261 cgcgtgcccg cggcagtgac aggtgccgat gccgcagggc cgctgctgca tccgaatacg
    10321 gtgacggcgc ccggccgact ctcgctccgg gagcggctcg aaggcgggtc attggcgatg
    10381 gtgttcgtcg ccggtcttgc actcgcgacg ccacccgtcg agtacctggc agtgctcatc
    10441 acgatcctgg cctcacgagc aaccgtcgct gcacaacttg gtgcggcgct catgttcact
    10501 ctcgtcgcgt tcacggtcgt cgaggttccg ctcatcggct acctcacgac gccggcgaag
    10561 acgctggacg tggtgcggcg actgaacgaa tggatacact cgcgccggca ggtcatcccc
    10621 tgcgtcctca tcggtgtagt cggtgccgta ctcatggcta ccggcatggg caaggtctga
    10681 cgagcgggcg aatcagcggc tgttgcagcg cttcccgcgg ctcaacgttg ccggggcggc
    10741 gtcagcgcgg gagacgatgt ccgaccggtc gcatgccgtc gacaagcagg gccaggtagc
    10801 ggcggccatt tccgcgtccg cccctacgca gcatggtcag ggtcatgacg acgatcgcgg
    10861 tgacgtcatc gactgtcagt tcgggccgga tcacgccggc gtcgcgagcg cgattgagga
    10921 cccgttcgat tgctgccaga tactcgcttt tggcgctcag gtcgaagacc gcggattcgc
    10981 gggccaggcc gaccagaacc gggtgcgcgg cgaaggcgtc aacggtggca ctcagcgtgg
    11041 cgacaaggtc tgtccacggg tctggacctc cctcgacgat ggccgttgcg acgtctgcgg
    11101 cgaaaacatg ctcggcgaca gcggctatga gctcttcacg tgtgcgaaag cgccggtaca
    11161 gtgtcgcgac gccgactccc gcgcgggcgg cgatctcatc cagtgagacg gtcgggccct
    11221 cctgctcgaa ggcctgggcg gcggcgtcga ggatgcgact gtaattgcgc gaggcatccg
    11281 accgtaaacc ccggttgttg tccgtcgcca tgttggcacc ataacaacgc gacaaggtca
    11341 gtccgcgatg agctgaagcg caccggacgg gcacaaactg atcgcgttac ggacgcgccg
    11401 ttgctcctcg gccggcacct cttcggcgtc gaccatgacg atgccatcct cgtcttggtc
    11461 gaacatcgcg gcggtcatta cgcacatgcc cgcgccgatg cacacgtctc ggtcggcaaa
    11521 gaccttcatg acagcctcac caggtcaccg acaacgaccg ggcgccgtag accagacttg
    11581 ttggcttgaa ggagatttcg gtgaggtcct cagccagttt caagccgcgg aatcggcgta
    11641 gcaatgccgg taacgcgatc cgcatctcca tacgggccag cggtgcgcca aggcaatggt
    11701 ggacgccgtg cccgaacgcg agatgcccgg gtgcgccacg acgaatgtcg aggctatcgg
    11761 ccgtctcgat gaagcgcgga tcgtggttgg ccgacggcaa cgacagcaac acgagagagc
    11821 cgccgggaat gctcaccccg ccgatctcaa cgtcggtggt ggcgatccgc ggaatggcgg
    11881 tctgcacgac ggacgcgtaa cggagcagct cctcgacggc cgggcctacc gcggcaggat
    11941 cgtcgcggac ggccgcgagc tgcgcggggt gctgaagcaa gaccacggtt cccagcgcca
    12001 acatgttcga tgtggtttcg tgaccggcga caaggagcag cgcgccgatc ccggcaagct
    12061 cgtcgtcgct gacttcgtcc ccgtgttcgc ggaccagcgt gccgattaga ccgtcgccgg
    12121 gcgcgcggcg cgcccgctcg acaagcgacc gcatgtagga acggctttca tcggccacag
    12181 tctggcgttc gactggtgcc agtgccatgt cgagctggcg cagactgcgc cgctggaagc
    12241 cgtcgcggtc ggcatacggc actccaagca actcgcaaat caccagcgac ggaacgggga
    12301 gcgcgaagct cgcgacgaga tcagccggtg cgccggcctc ttccattgcg tcgaggtgct
    12361 cgtcgacgat ttcgacgatg cgcgattcca ggtgattcat ctgccgaatc gtgaacatcg
    12421 gtgcgagcat tcgacgcagg cggtgatgct gcggcgggtc ggtggccaac agcgcccccg
    12481 gcggatcgtg tgcatcgatc ccgttctgtt ccgcggtcaa gccaccgaaa gtagtcgttc
    12541 tacggttgga aaatcgctcg tgatcggcga gcgcggcctt tatgtctgca tagcgtgtca
    12601 cgaggtatgc cggttccccg aatgccgtaa aggtggcgtg cactgcagcc gactcccgga
    12661 ttccacgaag gccggcggac ggttcgacgc cgtcgcgcat caagtacggc gggatcgctc
    12721 gcgtttggct cataccacca ctttacgtaa gtgatagaca actatcaatt agcgctagca
    12781 cccatctctc agtctcgtgg tccgagcggg acatcacgaa gtagcgatgg actaatcaca
    12841 cgccggcaga agggttttag accgcgccgc cgtctagccg cagcagtagg ctgcggcctt
    12901 ccggtcgacc tatcagttga tgcaaatggt gccgttggcg tgtgtgccac taccggtaag
    12961 gcacccattg ggaggatctt tgggcaggcc agggggtagg cagttttgcc tatagtcgtc
    13021 ccagtaccag ccgtatgggc agtcggcctg gttcatcgcg gccggggtgg tggccagcga
    13081 gaccaatccg gcgctcgccg cagcgacggc agtcgcgatt gccaatgtca gggtcttcat
    13141 attcgtcgtt ctccttgcac tcagcgtccg cgatccgaag ctgttctgtc ggatgcggaa
    13201 aacgcccctg ccgacgattg tcggcggcga accgactcgc cgcctccatg ccatccgcag
    13261 atccatctac cggctagcac gagttggatc aacgccgtcc gacagcgaaa cagtctgtgg
    13321 cacagggcga tagatgattg ggggcggtga gcgccgatat ggccgcacag tggctcggct
    13381 gccaaatatc ttgtatgaca gtcaatactg acgattcatc agcagaaaca ggcggatctc
    13441 agaatacggg ttggccggta tatcaatttg gcggtggccg taacgacatg tcgatgtgaa
    13501 acccggatgc tggcaatcag accgcgacag agcaatgaac tgcgcgtgat cggttgtgaa
    13561 gaccgagatc tcatgaagaa aggacgacgt ccagttcggc ggacgatccg ccgggaacag
    13621 atggaggaaa caccatggca taccagaccg ttaacccgta cgacaaccag atcgtggcca
    13681 cgtttgacga cctcaccgat gagcaagcga tgagtcttct tacgcaggcg cagaagacat
    13741 ttgagtcatg gagccagacg tcgttccccg aacgcaccgc cgtcatcaag cgcgccgcgg
    13801 cgatcttccg cgaacggtcc gacgagttcg cccggctgct taccctcgag atgggcaagc
    13861 tctacgctga ggccttgggc gaagtcgagc tcagcgcaca gatcttggac tactacgccg
    13921 agaacgccga ggcgttcctg gcccccgagc ccctcgacac cgccagctcc gatgacaacg
    13981 ccatgctggt cagcgcaccg ctgggagtca tcctgggggt tcagccctgg aacttcccgt
    14041 actaccagct ggcgcgggtg gcggggccca acctgatggc aggcaacgtg gtcatcgtca
    14101 agcacgcctc caacgtgccg caggcggcgg ccgccttcga aaagctgttg ctggaggccg
    14161 gcgcaccaaa gggcgcctac accaatgtgt acgccaccaa ggagcagatc aacttgctga
    14221 tcgacgaccc gcgcgtgcgc ggcgtcgcgt tgaccggcag cgagggtgcc ggcgcggtcg
    14281 tggccgcccg ggcgggcaag aacctcaaga agtccaccct ggaactgggc gggtcggatg
    14341 ccttcatcgc gctggccgac tgtgacctgg acaagactgt cgagtgggcg ttgcacggcc
    14401 gcgttttcaa cggcgggcag tgctgtgtgg cctcgaagcg gatcatcgtc gtcgacgaga
    14461 ttgccgacga gttcctggct aagtttcagg aggcgatgtc cgagctgaag gccggcgatc
    14521 cgttcgacga ggcgacgacg ctggccccga tgtccagcca gcgtgccgcc gacgacctgt
    14581 acgggcagat caacgccgca gtggaagcgg gcgcgaaggc gatttcatgc ggcgatttgg
    14641 ttccgacgac gggcgcgttc gtgcaaccga caatcctgac cgatatcacc ccggacaacc
    14701 cggcctacta ccaagagttc ttcgggccgg tggcgctgtt cttccgggtc aaggacgagg
    14761 acgaagcagt acggctggcc aacgactcac gcttcgggtt gggcggatcg gtcttcacca
    14821 gcgacgtcgc gcggggcatt gaaattgcca agcgcatcga caccggcatg gtgaacatca
    14881 accaccccgc ccgcctcaag gccgacctgc cgttcggcgg ggtcaagatc tcgggctacg
    14941 gccacgaact cgcccacctc ggcattgagg agttcatcaa caagaagctc atcaacgtcg
    15001 tcccgatcga cgctccggcc tgatcactcc ctgaccaaga gaagaaggaa taacaaacat
    15061 gcctaacttc accgtgggga aatacctcgc tgcccggttg gagcagatcg gcctgaagca
    15121 ctacttcatg gtccccggcg actacaacct ggtcctgctc gatgagctgc tggagaacga
    15181 gaacatcgag cagatcgggt gctgtaacga actcaatgcg gcctacgccg ccgagggcta
    15241 tgcgcgggtc aacggcgccg gagccgtgct gaccaccttc aacgtgggtg cgttctccgc
    15301 actgaacggc gtggccggcg cctacgccga acgccttcca gtgatcgtgg tttcctcggg
    15361 acccaatacg aatgacgtgg gctccaatca cgtccttcac cacaccatgg acaacggcga
    15421 cctgtcggat cagtacgaga ttttccggaa ggtgacctgc aaggcggtgc ggatcgtgca
    15481 ccccgacaca gcacctgccc ttatcgatga ggcgatcagc gccgcgctgc gcgagcgcaa
    15541 gcccgcctac atcgagatcc cgtgcaacct gtcggctgcg ccgtgctctg agcctgcgcc
    15601 gttcgacaca ctgccggcct gggagcccag caacgcgcga atgctgaccg acgccgtcgg
    15661 ccgagccagc gatcttgtgc agcaggcgaa gaagccaatt ctgttggccg gggtgcacct
    15721 gcgcgcgcac ggtgcgatcg acgccttccg cgagctggcc gaggcgctgg gctgcgcggt
    15781 agcggtgatg ccgaacgcca agggcttctt ccccgaagac catccgcaat acgtcggcat
    15841 ctactggggc gaggtgagca gcccgcagtg tcaggccatc gtcgactggg ccgacctcgt
    15901 cgtggccgca ggggcggtgt tcaacgacta caccacggtg ggttggagcg cgcagccagc
    15961 ccgcgggcgg ctcatcggcg ccgagcccag gaaggtgcag ttgcccgacg ccgaatacca
    16021 cggagtggcg ctggccgagt tcttatccga tctcgccaag aaagtgccag ccaatgccgc
    16081 gacactgacc caataccacc ggctccaccg gaccacgtcc gcccaggtgg cggtcgcgga
    16141 cgtcgacccc acggcgccgc tgagccgtgt cgagatgtgg cgccagattg agcaggatct
    16201 cgacgagaag tccaccctgc tggtggaaac gggtgactcc tggctcaacg ggatgtacac
    16261 ccggctgccc ggcggtgccc gattcgagat cgagatgcag tacggctcga tcggctggtc
    16321 ggtgccggcg accttcggct acgcgaaagg ccttgagccc gaccgccgct tggtttcggt
    16381 tatcggcgat ggttccttcc agctgactgc acaggaagtg tcgaacatga tccgccacgg
    16441 ccaggagctc atgatcttcc tggtcaacaa ccgcggatac gtgatcgagt cagcgatcca
    16501 cgacggtccg tacaactact acaagaactg ggactacgcc ggtctcatcg acgtgttcaa
    16561 cgcaggagag ggcgctgggc tcggcttggt cgccagcacc gctggagaac tggccgaggc
    16621 gatcagcaaa gcacacggcc acaagggcgg gccggtgttg atcgagtgcc gaatccccaa
    16681 tgacgacgcc agccctgagc tcatctcttg gggatccaag gtggctagcg tcaacggtcg
    16741 accggacccg cggttctaga ggaacgaagc cgacaggccg tttctcatcg acgacgatcc
    16801 tcccgccagg cgctcggcgg gaggatcgtc gctcgttcgg cttcgccatc aggtgaaaac
    16861 cgttacgtcc ccgtgtaacg cttagcggct cagtgccgtc tagtagggct ctaagagacg
    16921 ttcggcagta ccggcatccg ctcttcagcg acggtccgcc gtgtgcacaa ttcggctgct
    16981 atgcaagcga ttacctgtcg actcacgagc atcccgagat ggccgacacc ttcaaaggtg
    17041 atggtttccg cctgagcgtg cgacggcact gcgcgcaccc cgggcacaat gatgtcgagt
    17101 gctgccgtca acgacagcca ccggatcccg tcgggcaacg gggcggacgc gagccggttc
    17161 aacactggcg agccggcgcg aagttcccgc acgatttcga ggaacggcag cacacccgcc
    17221 cacggcgacc ctccgaatgg cgagccgagg gtgatgaccg tgtcgactcg cccagtcaga
    17281 cgactgtcgg cgatggcctg tgcaatgacg acaccgccca gactgtgtcc aacgagatgc
    17341 accttgtcgg tgccggttgc caccaagatt ctctccacct cggccaccag tcgctcagcc
    17401 agctgctgca ccgatgttcc gaccggcgga tagctcatcg cttcgacggc cacgcctcgg
    17461 gctttgaggg cgttggcgac caaggcccag cttgacttgg tgcccgcgaa cccatggaca
    17521 agtagaacag gccgggtcgt gggcgcggtg agagctttca cggtcaggga actgcttgca
    17581 gtgtcgtagc taccgccgag catccggagg ggtgccatcg ccaccgtcga catcatcatg
    17641 gcggcctcgt tcgcctgcac cgccgcagca cgccgcaccg tctggaccgc cgcgcgggca
    17701 gcgtcgagta ggtcctggag ggccctcggg ccgctttgcg tgcgcgccgc gcagacctgt
    17761 tctgcccgtg ggctttccgt cgatcctagt cgcaccatgc cctacccctt tgcggtgtga
    17821 tgggtattac ctcgagcatg gcggttggcg gatcgggtgt cttcaatgcc agcagcagtt
    17881 tgctgtgcgg gcaagcagga gccgtctcta cgagctagcg ggtaggtgtg cacaagcaca
    17941 ttgcgtagca acgtttttta tcgccgtgca cattcgggca tgaccgcccg caagagtgcg
    18001 aagtgctatc ccctgttcgg ccaagcgtca gaaagccggc gtgttgtcag aggtttcggt
    18061 cgcgcgctgc agcccagagc tcacacttgc gctgcgccca ttccaccgtg aagccggtga
    18121 ctacagcggt ggtcagggtg acgattgaaa tcatcatcat gactcctcac tacggcggac
    18181 gttggtatat acatcgtcac gccgaaaggg cccgaagtct tcggtgttgg cgcccatcta
    18241 tggtggctgc agcccgcaat cccggcttgg ggctagccgg tagtcatgtt ctttgcagat
    18301 acaagtaaac aagcgcatca gcgccgtcag catcattcgg caccccccgc ggtacatgtt
    18361 ggcagatgct cgagccgagt cgatatcgtg acggatcagc acaattacat tgcagtacag
    18421 catgtttcgc tgcatatctg cagccacaac tcagcgacca cttcgtctcc gagagtaatt
    18481 gcgtgctagc agatatctca acgggtgtta gccccaaagc aatacggctg ccgacgatgc
    18541 atgatgacgt caagacgaga cacaatgctc actcgaagga cagtgatgta caggcacatg
    18601 aaattcgctg cggccagcgc tggggcacta gccctattcg gtatgggtgg cgcggcggcc
    18661 gttgacggaa ggcacgaatg aaagccgcag ttgtaagagc cctaggtcag ccacccgagt
    18721 atgccgattt ccccgaccca aaggcccgcg atggtgcaat cgtggcgacg gtcgaagccg
    18781 cggcgctgaa gaacctcgac cgggctcttg tgtctggcac gcactacaac agcgcacgcc
    18841 tgccgctgcc cggcatcgcc ggtgtcgacg gggttgcccg gctcgacgat gggcgattgg
    18901 tctatgccaa cgcgctgcct gcgcagggaa tgatggccga gaagaccctc atcaatcccg
    18961 cctcggccgt cgaattgccg gagggcatcg atcctgtggt tgccgcggcg gtgcccaatc
    19021 ccgccctgtc cgcgtggttg tcgttcgagc accgcgccca tatgcgcgag ggccaacatg
    19081 tgctggtact gggggccacc ggcgtcacgg ggtcactggc cgtgcaactg gccaaggcgg
    19141 cattcggtgc cggccgggtc gttgcggccg gccggaacgc agcgcggctc gagtggctgc
    19201 aaaccgttgg cgctgacgag gttataccgt tggaaacaga ggatttagcg acatgcattg
    19261 ccgcacaaca tcgaatgcaa ccctttgacg tggtacttga ctacctgtgg ggtccccctg
    19321 ccgagcaggc gttggcggcg ttgggcaacg tcgacccgaa cgcaacgtcc cacgtcaccc
    19381 gtttcgtgca gataggggat atggcagggt cgagtatcac ccttccggcc ggaatcttac
    19441 gcagcgcggg aattgagttg gtgggcatgg ggttcggcag tgtgccggcc gccgcatggg
    19501 ctcgcgcagc tgctgaccac ctgccgcgcc tattcgatat ggttgccgac ggtcggctgc
    19561 atctgcagac cgaacggcga ccgttgagtc aggtcgagga cgtctggacg cagaaggaac
    19621 cctccggcac ccgagtggtt ttcgtcccag gacagtagtc gcgggcagct tgagacgttc
    19681 aggccgggat cagcctgagt ggcaggtgct cgtaacagcg gatgatgttg ttgatcgtcc
    19741 atgtcggctc gccggcgagt tcgatccggc tcacctgctg catcagcgca gcgagcatcg
    19801 cctgcatctc gagtcgggcg agcccctgcc cggcgcaggc atgcgtgccg tgcccgaagc
    19861 cgagatggcg cgtggcgtta cgccggatgt cgaaggcatc gggttctgtc cactccagct
    19921 cgtcgcggtt cgccgacgcg tacaacacta gaaaccgcga gccggccgga ataccgacac
    19981 ccgcgatttc agtcggctgc agcgactttc ggctgaacgc ccgcaacggg gactcatgcc
    20041 gcagcacctc attgatcaca ttgggcagta gggagggctc cgcacgcagc atgtcccact
    20101 gttccggatg ggaagcgaag agtgccagtg cactggcgat cccgctgatc gtggtgtcga
    20161 gcgacggaac gagataatcg atcatcagcg ccgagcattc gctgtggcga aggttgccgg
    20221 catcggcggc ggtgaggacg tcatgaccca tgcttccgcg gatcaccgac cgtcggcgca
    20281 ccacgttttt agagaaccgc agcatctgca tgctggccgg caccgacttg agggaaaatc
    20341 ggttcagcgg gccaagcaca tcgaatgtgg ctccgcccca acgcagcagg tggtggcggc
    20401 ggtcctccgg ccagccgacg agatcgggca ccacagagag cggcagcgcc gtcgcgagat
    20461 ccgtgacacc gtcgacgtgt tgacgtctca cggcctccgc gaccagctgc tcggcttgac
    20521 tggttacgac gtcactcatc gtccgcaagg cccgcggcag gagccggtgc gcgacgagct
    20581 tgcgccgctg gtcgtgttcg tcgccgtcac tgttgagcgt tgtgccgcgg gacagccggt
    20641 taaagacccg gttcagtcca accccttcgc cggagatgaa cgttgtgtcg tcccgtagca
    20701 ccgccttgca ctcggcgtag cgcggcagag cgtagacccg ctgcttggcc agccacacca
    20761 ccggaccgag tcgccgtagc cgcgcatagt gcgggtgggg gtcgacgatc gcctgcgtcg
    20821 catacaggtc cggctcgtag accgggacgt cggcgggcct acgcatcagc gcgggtatcg
    20881 gcggctgacg cgaaacccgg taccgcgttg cagacttcgg ataggttacg gctggcgcac
    20941 agcacagtcc gatccggtcg caccacggcg gccgtggcgc ggcccgcccg cagccagagg
    21001 tccagttccg tgccgggatg cgcggttacg accaccgcgc cgcggcgggt cagctcgtcc
    21061 tgctgggacg ggtcgagcgg tgcggacgtg accaccgcga acctggcgcc gacgacttcg
    21121 tcaaaccgtt tgccgtctac cagaacagga ttcgggcaca accggcccgc caactcccgc
    21181 cgtccggatg atttcacgac cagctcactg ctgtgcaagg cgggtgtcgc gctgtcggcg
    21241 atctttttcc gcactcccgg gatcagatgc agccgcggaa ccaccgctcg gcggaggaag
    21301 ttgcccacct cgccacccgc ggtcatcgcc gctcccaccg cgagcgcgta ccgaatcata
    21361 taccgcgcgt gcggtatacg ctcctgctca tacgtatcca gggcggactc cggcaaccat
    21421 ccgtttaccg cgccggcaag cttccacgcg aggttcgtcg cgtcgcgcag gccagcgccc
    21481 atgccctggc cgatgaacgg aggtgtcagg tgggcggcgt cgccgaggat gaacatgttg
    21541 ccacggcgcc agcggttcgc cacctgggcg cggaacgtgt actcggcgac gcgcacgagc
    21601 tgcagctgtt ctggcgcaac gccttccacc caaggttgga tcagcgggta gagcgccgcc
    21661 gtagtgctgt agtcgtcggc cacctcgtcc gggaggagtc ggaactccca ccggtagcgg
    21721 gtatcgccga tccgcatgta ggtggccgct cgatgtgggt cgcagacctg gtggacgctc
    21781 tccccctggt tcaactccgc ggtcgtcgcc acgtcgacca ccagccaacg ctgctggaac
    21841 ttcaagtcct ccatggatgc gccgatcgct gcgcggacca ggctgttcgc cccgtcgcag
    21901 ccaagcacat atctcgtctc cacccggtgc tcttcgccgc tgacccgatc ggtgaacgcg
    21961 acctgcactc ggtcaggggc gatctggacc gcttccatca cctcggcatt gcggatgagt
    22021 tcggggcgat ctcccgaccc gcgcgtggcc tgcagctgcg agaccggaac atgcgggt
//
LOCUS       DASRJA010000710         8690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_948169,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000710 DASRJA010000000
VERSION     DASRJA010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8690
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948169"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFC01_32050"
     CDS             complement(<1..372)
                     /locus_tag="VFC01_32050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZN84289.1"
                     /translation="MELVTIGDAAEMLALNASALRYYEDRGLVTPERRGGKRMYSREQ
                     LRRLAFIQLMQRLGIRLDAASAVLDEPSDQWRDVVREQIAAIEDLIARANGARDFLEH
                     ALACPADHPVDECPHMIEALDK"
     gene            418..1035
                     /locus_tag="VFC01_32055"
     CDS             418..1035
                     /locus_tag="VFC01_32055"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZN84290.1"
                     /translation="MAPKPRILDPSTNNPFAHPSGWLGRMAGRFMLWTNKQDDVVHVL
                     AVQPGNDVLEIGYGPGGLIRLLAERTDAASIRGVDPSPEMRDQASVHNRNAVRTGRVA
                     LDLGTADRTGLADASVDRAVSVNNVAIWPDLEAAVDELHRVLRPGGIVVIAWHGGTSP
                     SRIARSLRLSEGELGRIEHVLRDRFGEVSRKQLASLDVFTAVRSG"
     gene            1077..1646
                     /locus_tag="VFC01_32060"
     CDS             1077..1646
                     /locus_tag="VFC01_32060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84291.1"
                     /translation="MRHYHLHGRRSGRSGGWQQAEQPDASDAADWFAGRLPDGWFAGD
                     PTVVVDREEITVIGRLPVADGEESEAKASGRVARFREETRPERMRIADEAEARYGRKV
                     AWGIEIGAVGDENYQRILFTHIAVPVMTRLKQPERQVLDTLVDAGVARSRSDALAWSV
                     RLVGEHADEWLGKLREAMKNVDDLRAEGP"
     gene            complement(1649..3814)
                     /locus_tag="VFC01_32065"
     CDS             complement(1649..3814)
                     /locus_tag="VFC01_32065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN84292.1"
                     /translation="MSTRTVHTFCRYCLASCGVEVTVEDNRVTKISADKQNPHTWQDF
                     CAKGRTANQLVEHPRRILNPMRRVGDSYVEAGWDEAINDIAARMNALIEADGPDAIGV
                     YYGNPSGFSSSNIIFMNAWLDAVGTHNRYFVGSVDQNAMHVVASAMYGSMLMAPVSDI
                     DNCDYFLLVGTNPAVSAWNWLETVPGGWRRALERQKQGATIVVVDPLRTETAEKADVH
                     LAVRPGQDWALLLGMVKVILDEGLEHRQDCIGLATGVDDLRMLVGDADLDDLASRCDI
                     PRVQIEQVARDFAAARAAMGVTRTGVSLHLAGTVAEWLGHVLNVITGRMDRPGGRRYE
                     PGYVDALRLSEMAKARPHKSRLAGREMVAGAHAVSELPDEITTPGPGQIKAMLINCGN
                     PVVSGPDGAKLDEALAQLDLLVAIDFVQRESHRHAHWLLPAVHWLERDDLLAFTSNMH
                     DEPYVQYGVKAVDPPPGARQEWRIFVDLAIAMRKPLFGAKGLNGFIKATRRLARLTRR
                     PALEFGPHWIDRLVVATGRKVNGRRIKWRDVMAHPHGWVLGPREFGHFRDALRTDDKK
                     VHAAPPEFVSRTRELLAEPAPQAPSEYPFQLANRRNRHSMNSWLNELPGLHPSGKHNE
                     VVIHPDDAVALGISDGDRVKVFSAVGAIELEASVNERPRRGVVVIDHGWGSRIFDPRG
                     GSQPESYGANRNLLIDGGPIDPLSQTAALSSSYVGVERV"
     gene            complement(3831..5741)
                     /locus_tag="VFC01_32070"
     CDS             complement(3831..5741)
                     /locus_tag="VFC01_32070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN84293.1"
                     /translation="MTAGPMARPIRGVVQAPTERSRDFKGSAIRLVKRLTPQRGLTAM
                     VILLGVGGIAIGVIGPRILGHATDLLFNGVIGRQLPAGLTKEQAIAAARARGDNTFAD
                     LLSGMNVVPGHGVDFGAIGRTLLLALGLYLVAALMVWLQARLLNVAVQRTMVALRADV
                     EQKVHRLPLRFFDSRQRGEVLSRVTNDVDNIQQSLSMTISQLLTSLLTVLAVLVMMLT
                     ISPLLTLLTVVTVPLSLWVTRAIARRSQRLFVAQWANTGRLNAHIEETYSGFTLVKTF
                     GHRARALEQFQERNDDVYRTSFGAQFFSGLVGPATTFIGNLSYVAVAVVGGVQVATGQ
                     ITLGSIQAFIQYVRQFNQPLTQVAGMYNTLQSGVASVERVFDLLDAEEESPDPDVTLP
                     SLDGGPRGRVEFQHVNFAYLPRTPVIEDLSLVAEPGSTVAIVGPTGAGKTTLVNLLMR
                     FYDVDSGRILVDGVDISSVSRSSLRSRIGMVLQDTWLLGGTIYDNIAYGRPDASEDEV
                     IAAAKAAYVDRFVHTLPDGYQTNVSDDGGNISAGEKQLITIARAFLARPQLLVLDEAT
                     SSVDTRTELLIQHAMGELRRDRTSFIIAHRLSTIRDADVILVMEAGRIVERGSHEELL
                     ARHGEYWAMTQA"
     gene            complement(5738..7480)
                     /locus_tag="VFC01_32075"
     CDS             complement(5738..7480)
                     /locus_tag="VFC01_32075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN84294.1"
                     /translation="MLWALLRRYTGPYRSLLGIVAGLQLISTLASLYLPTVNAAIIDD
                     GVAQGNLRRIVELGGVMLAVTGLQVVCAIGAVYFGSRAAMGVGRDLRSTIFGHVTSFS
                     AEETARFGAPSLLTRTTNDVQQIQQFVQLTCTMLITAPIMSIGGIFMAIHQDAGLSWL
                     LLVSVPVLALANYWIVSHLLPIFRRLQRQIDGINRVMREQLSGLRVIRAFAREPFERN
                     RFGEANQALSDTALEAGRWQALMLPVTTLVINISSVALIWFGGLRIDDGKMQVGSLIA
                     FLSYFMQILMAVLLATFMLVLLPRASVCAERITEVLSTSPGIASPRNPVRLDAVRGEI
                     RLDSATFSYPGADRPVLQDVSLTAGAGSTTAIVGSTGSGKSTLISLICRLYDVTDGSV
                     RVDGVDVREYDTEQLWSAIGLVPQRGYLFSGTVADNLRYGKADATDEEMWGALRVAAA
                     DDFVRAHPDGLSMPVAQGGINFSGGQRQRLAIARAVVRKPAIYLFDDAFSALDVHTDA
                     RVRASLREVSAEAVVVIVSQRISTVATADQVVVVDDGQVVGIGSHETLLTTCPTYAEF
                     ADSQSLGAGAGGHG"
     gene            7675..8181
                     /locus_tag="VFC01_32080"
     CDS             7675..8181
                     /locus_tag="VFC01_32080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HZN84295.1"
                     /translation="MIPVFAACSDSSGSTSPSVPSSPPPSQNASHGPMFPECGGISDQ
                     VLAEQTRVTGLVNTARNSVGCQWLQGGGILGPHFSFTWYRGSPIGRERKTEELSRASV
                     EDINIDGHEGFIAIGEDPTLGNNLCEVGIQFQDDFIEWSISFSEQPFPDPCDVAKELT
                     RQSIVNAK"
     gene            8178..>8690
                     /locus_tag="VFC01_32085"
     CDS             8178..>8690
                     /locus_tag="VFC01_32085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HZN84296.1"
                     /translation="MSRSFGRRALAGLIAAVSVLTLLTGCTKTVEGTAAKSGSGDVPR
                     NDDSAKQYPNLLKECEVLTEDILAKTVGADPLDIQSTFVGAVCRWQAANPAGLIDITR
                     FWYEQGSLDNEKKVAQSLNYQIENRSIAGVPSIVMKPNDPNGACGVASDAGGVVGWWV
                     NPQTPGIDACG"
BASE COUNT         1523 a         2912 c         2810 g         1445 t
ORIGIN      
        1 tttgtcgagc gcctcgatca tgtgggggca ttcgtcgacc ggatgatcgg ccgggcaggc
       61 cagcgcgtgt tcgaggaagt cgcgcgcgcc attcgcccgg gcgatgaggt cctcgatggc
      121 ggcgatctgc tcgcgtacca cgtcgcgcca ctggtcactc ggctcgtcga gcacggccga
      181 ggccgcgtcc aggcggatgc cgagtcgctg catcagctgg atgaacgcca accggcgcag
      241 ttgttcacgg ctgtacatgc gctttccgcc gcggcgctca ggcgtcacca accctctgtc
      301 ctcgtagtag cgcagtgccg acgcattcag tgcgagcatc tcggcggcat cgccgatcgt
      361 cacgagctcc atctcttcat ttgacttcaa cccggcttga agtgccagcg tggagtcatg
      421 gcaccgaagc ccagaatcct cgacccgtcg accaacaacc cgttcgcgca tccgagcggc
      481 tggcttggcc gaatggcagg tcggttcatg ctgtggacga acaaacagga cgacgtggtg
      541 cacgtgctcg cggtccaacc gggcaatgat gttctcgaaa tcggttatgg gcccggcggt
      601 ctcatccggc tcctcgccga gcgcaccgac gcggcgagca tccgcggcgt cgacccgtcg
      661 ccggagatgc gcgatcaagc cagcgtgcac aaccgaaatg ccgtccgcac gggccgggtc
      721 gcgctcgatc tcggtaccgc cgaccgcacc ggtctggcgg acgccagcgt cgaccgcgcg
      781 gtctccgtga acaacgtggc gatctggcct gatctcgagg cggcggtgga tgagctgcac
      841 cgggtcctac ggccgggcgg catcgtcgtc atcgcctggc atggcggcac gtcgccgtcc
      901 cggatcgcca ggtcgctgcg cctgtccgag ggcgagctcg gccgcatcga gcacgtgctg
      961 cgagatcgtt ttggcgaggt ctcccgcaag cagctggcca gcctcgacgt attcacggcc
     1021 gttcgctcgg ggtgaaagcg gtaccaccag gcttgtaacg gtgtaatcat gtaatcatgc
     1081 gacactatca ccttcatggc cgacgctctg gccgctccgg cggttggcaa caggccgagc
     1141 aacccgatgc aagcgacgcc gccgactggt tcgccggccg gctccccgac ggctggttcg
     1201 ccggcgaccc gacggtcgtc gtcgaccgcg aggaaattac cgtcatcggc cgcctacctg
     1261 tagcggacgg cgaggaaagc gaggccaagg cttcaggcag agtcgcccgc ttccgtgaag
     1321 agacccgtcc cgagcgcatg cgcatcgccg acgaggctga agcccgctac ggccgcaagg
     1381 tcgcgtgggg catagaaatc ggggcagttg gtgacgaaaa ttatcagcgg atcctgttca
     1441 cccacatcgc cgtacctgtc atgacgcggc tcaagcaacc cgaacgtcag gtgctcgaca
     1501 cgctggtcga cgccggggtc gcgcggtcgc gctccgacgc gctggcctgg tcggtccggc
     1561 tcgtcggtga gcatgccgac gaatggctcg gcaagctgcg cgaggcgatg aagaatgtag
     1621 acgatctgcg cgcggagggc ccgtagctct acacgcgttc gaccccgacg tacgacgagc
     1681 tcaacgcggc ggtctgcgac agcggatcaa tgggtccgcc gtcgatcagc aggttgcggt
     1741 tggcgccgta ggactccggc tgcgatccgc cgcgcgggtc gaaaatcctt gagccccagc
     1801 catggtcgat gacgacgaca ccgcgccgtg gccgctcgtt gaccgacgcc tcgagttcga
     1861 tcgcaccgac ggcggagaac accttcaccc ggtcgccgtc gctgatgcca agcgcgaccg
     1921 cgtcgtcggg atgaatgacc acctcgttgt gttttcctga cgggtgcaga ccgggcaatt
     1981 cgttgagcca tgagttcatc gaatggcggt tgcgccggtt cgccagctgg aacgggtact
     2041 cgctaggtgc ctgcggggct ggttcggcga gcagctcgcg ggttcgtgat acgaattccg
     2101 gtggcgccgc gtgcactttc ttgtcgtcgg ttcgcagcgc gtccctgaag tgcccgaact
     2161 ctcgcgggcc caacacccag ccatggggat gcgccatcac gtcacgccat ttgatcctgc
     2221 ggccgttgac ctttcgaccc gtagccacga ccagtctgtc gatccagtgc ggcccgaatt
     2281 cgagcgcggg cctgcgggtg agccgcgcga gccggcgggt ggccttgatg aagccgttga
     2341 gtcccttggc gccgaacagc ggcttgcgca tggcgattgc cagatcgacg aagatgcgcc
     2401 attcctgtcg ggcacccggt ggcgggtcga ccgccttgac gccgtactgc acatacggct
     2461 cgtcgtgcat attgctggtg aacgcgagca ggtcgtcgcg ttccagccag tgcacggcgg
     2521 gcagcagcca gtgcgcgtgg cggtggctct cgcgctgcac aaagtcgatg gccaccaaca
     2581 gatccagttg cgccaacgcc tcgtcgagct tcgcgccgtc gggccccgag accaccggat
     2641 tgccgcagtt gatcaacatc gccttgatct ggccgggccc tggagtcgtt atctcatcag
     2701 gcaattcgct gaccgcgtgc gcgccggcga ccatctcgcg gccggccaac cggctcttgt
     2761 gcggtctcgc cttggccatc tcggataacc gcaacgcgtc gacgtagccc ggctcgtacc
     2821 tgcggccgcc gggtcgatcc atccggccgg tgatcacatt gagcacgtga ccgagccact
     2881 cagcaactgt gcccgcaagg tgcagggaaa ccccggtccg cgtcaccccc atcgctgcgc
     2941 gtgcagcggc gaaatcgcgg gccacctgct cgatctggac ccgcgggatg tcgcagcgcg
     3001 aagccaagtc gtcgaggtcc gcgtcaccga ccaacatgcg caaatcatcg acgcccgttg
     3061 ccagaccgat gcagtcctgg cggtgctcga ggccttcgtc gaggatcact ttgaccatgc
     3121 ccaggagcaa cgcccagtcc tgtcccggcc ggaccgcgag atggacgtcg gccttctcag
     3181 cggtctcggt tcgcaacggg tcgacgacga cgatcgtggc accctgcttc tgccgctcca
     3241 aagcgcgtcg ccatccaccc ggcacggtct ccagccaatt ccatgcgctc acagcgggat
     3301 tggtgccgac gagcaggaag tagtcgcagt tgtcgatatc ggacacaggg gccatcagca
     3361 ttgagccgta catcgcggac gcgacgacgt gcatggcgtt ctggtccacc gagccgacga
     3421 agtaccggtt gtgggtgccg acggcgtcca gccacgcgtt catgaagatg atgttcgacg
     3481 aggagaagcc ggacggattg ccgtaataga cgccgatcgc gtcgggaccg tcggcctcga
     3541 tgagcgcgtt catccgtgcg gcgatgtcgt tgatggcctc gtcccacccg gcctcgacat
     3601 aggagtcgcc gacgcggcgc atcggattca agatccgtcg agggtgctcg acgagctggt
     3661 tggcggtgcg gcccttggcg cagaagtcct gccaggtgtg gggattctgc ttgtcggcag
     3721 agatcttggt gacccgattg tcctcaaccg tcacttccac accacacgag gccaggcagt
     3781 accggcagaa ggtgtgcacc gtgcgggtac tcatgcggtc atcctgcacc tcaagcctgc
     3841 gtcatggccc agtattcgcc gtgacgagcc agcagctcct catggctgcc gcgctcgacg
     3901 attctgcccg cctccatcac caagatgacg tctgcatcgc ggatcgtcga aagccgatgc
     3961 gcgataatga aactcgtgcg atcgcggcgc agctcgccca tagcgtgctg aatcagaagc
     4021 tcggtgcggg tgtctaccga gctggttgcc tcgtcaagga cgagcagttg cggccgggca
     4081 agaaaggcac gggcgatggt gatcagctgc ttttcgcctg cgctgatgtt gccgccgtcg
     4141 tcactgacgt tcgtctggta gccgtcgggc aacgtgtgta caaaccggtc gacgtaggcg
     4201 gccttcgcgg ccgcgatcac ctcgtcctcg ctggcatccg gccgtccata agcgatgttg
     4261 tcgtagatcg tcccgcccag cagccaggta tcctgcagca ccatcccgat ccgggagcgc
     4321 agcgaggacc ggctgaccga cgagatgtcc acgccgtcaa cgagaatgcg gccggagtcg
     4381 acgtcgtaga accgcatcag caggttcacc agcgtcgtct tgcccgcgcc ggtcggcccg
     4441 acgatcgcca cggtgctgcc cggttcggcc accagagaca ggtcttcgat caccggtgtc
     4501 cttggtagat aggcgaaatt cacgtgctgg aactcgaccc ggccgcgcgg accgccgtcc
     4561 agcgacggca gcgtcacgtc cgggtcgggc gactcttcct cggcgtcgag cagatcgaaa
     4621 acccgctcca cgcttgccac cccggattgc aacgtgttgt acatgccggc gacctgagtc
     4681 agcggctggt tgaactggcg gacgtactgg atgaacgcct ggatgctgcc cagcgtgatc
     4741 tggcccgtcg cgacctgcac gccgccgacc accgccaccg ccacgtaact caggttgccg
     4801 atgaatgtgg tggccggccc caccaatccc gaaaagaact gcgcgccaaa gctggtgcgg
     4861 tagacgtcgt cgttgcgctc ctggaactgc tcgagcgccc tggcccgatg accgaaggtc
     4921 ttgaccagcg tgaagccgct gtaggtctcc tcgatgtgcg cgttgagccg gccggtgttg
     4981 gcccactgcg cgacgaagag ccgctgcgag cggcgggcga tcgcccgcgt cacccacagc
     5041 gacagtggaa ccgtcacgac ggtcagcagc gtcagcagcg gcgaaatggt cagcatcatc
     5101 accagcaccg ccaacactgt cagcagcgag gtcaacagct ggctgatcgt catcgacaat
     5161 gactgctgga tgttgtcgac gtcgttggtg acccggctca gcacctcacc acgctgacgc
     5221 gagtcgaaga agcgcagcgg cagccggtgc accttctgct cgacatccgc ccgcagcgcc
     5281 accatcgtcc gctgcaccgc gacgttgaga agccgggcct gcagccaaac catcagcgcg
     5341 gcaacaaggt acagccccaa cgccagcagc agcgttcggc cgatcgcgcc gaagtccacg
     5401 ccgtggccgg gtaccacatt catgcctgac agcaggtcgg cgaaggtgtt gtcgccgcgt
     5461 gcgcgtgcgg ccgcgatcgc ctgttccttc gtcagcccgg cgggcaactg gcgcccgatc
     5521 acgccgttga acaacaggtc ggtcgcgtgg ccgaggatgc gcgggccgat cacgccgatc
     5581 gcgatgccgc cgacacccag caggatcacc attgcggtca gcccgcgctg cggggtgagc
     5641 cgcttgacca atcggattgc cgatcccttg aagtcgcgtg accgctccgt cggcgcctgc
     5701 acgacgccgc ggatcggacg cgccatcggt ccggcggtca tccgtggcct cctgctccgg
     5761 cgccgagcga ctgcgagtcg gcgaactcgg cgtatgtcgg gcatgtagtc agcaacgtct
     5821 cgtgtgatcc gatacccact acttggccgt catcgacgac gacgacctga tcggccgtcg
     5881 caaccgtcga aatgcgctgg gacacaatca ctaccaccgc ctcggccgac acttcgcgca
     5941 gcgaggcgcg gacccgtgca tcggtatgca cgtcgagcgc ggagaacgca tcgtcgaaca
     6001 ggtagatcgc cggcttgcgg accacggccc gcgcgatcgc caaccgttgc cgttgtccgc
     6061 ccgagaagtt gatgccgccc tgagcgaccg gcatcgacag cccgtcgggg tgggcgcgga
     6121 cgaagtcgtc ggccgccgcc actcgcaacg cgccccacat ctcctcgtcg gtcgcatcgg
     6181 ccttgccgta gcgcaggttg tcggcgaccg tgccggagaa caggtaaccg cgctgcggca
     6241 ccaggccgat cgccgaccac agctgctcgg tgtcgtactc gcgcacgtcg acgccgtcca
     6301 cccgcaccga gccgtcggtg acgtcgtaaa gccggcagat cagcgatatc agcgtcgact
     6361 tgcccgaccc ggtcgacccg acgatggccg tcgtactccc ggctccagcc gtcagcgaaa
     6421 catcctgcag cacaggtcga tcggcgccgg gataactgaa cgttgcgctg tccaagcgga
     6481 tctcgccgcg gacagcgtca agtcgcaccg gattccgcgg gctggcgatt cccggcgagg
     6541 tggacagcac ctcggtgatc cgttcggcgc acaccgacgc gcgcggcagc agcaccagca
     6601 tgaatgtcgc cagcagcacc gccatcagga tctgcatgaa gtaggacagg aacgcgatca
     6661 gcgagcccac ctgcatcttt ccgtcgtcga tccgcaagcc gccgaaccag atcagcgcaa
     6721 cgctggagat gttgatgacc agcgtggtga ccggcagcat cagcgcctgc catcggcccg
     6781 cttccagcgc ggtgtccgac agtgcctggt tcgcctcgcc gaaacggttg cgctcgaagg
     6841 gttcccgagc gaacgcccgg atcactcgca ggccggagag ctgctcgcgc atcacccggt
     6901 tgatgccgtc gatctgacgt tgcagccggc ggaagatcgg cagcaggtgc gacacgatcc
     6961 agtagttggc cagcgccagc accggcacgc tgaccaacag cagccacgac aagcccgcgt
     7021 cctgatgtat cgccatgaag atgccgccga tcgacatgat cggcgcggtg atcagcatcg
     7081 tgcaggtcag ctggacgaac tgttggatct gctggacgtc attggtggtt cgggtaagca
     7141 gcgacggggc gccgaacctc gccgtctcct cggccgagaa gctggtgaca tggccgaaga
     7201 tcgtcgagcg caggtcgcgt ccgacgccca tcgcggcgcg cgacccgaag tacaccgcgc
     7261 cgatcgcgca caccacctgc agtccggtca cggcgagcat gacgccgccg agctcgacga
     7321 tccttcgcag attgccctgg gcgacgccgt cgtcgatgat cgcggcgttg accgtgggca
     7381 ggtacagcga cgcgagcgtg ctgatcagct ggagccccgc gacgatgccg agcaacgagc
     7441 gatacggtcc cgtgtaccgt cgcagcaacg cccacagcat cccgttactg tcgcacaccc
     7501 ctcgcacgga gctgtgttcg gcagggccac ctgagaattc gataaatgag ctggtcaacc
     7561 ggcctgtcgg tggttgaggt attgggtgcc gctacagtgc atggcgtgac cccgagcaag
     7621 tgcgcgaccg gtaggccgcg gtccgctaag gcgctggccg tggcggccgc cgccatgatt
     7681 ccggtgtttg cggcatgctc cgacagctcg ggatcgacgt cgcccagcgt tccgtcctca
     7741 ccaccgccct cacagaacgc gtcgcacgga ccgatgtttc cggagtgcgg cggcatcagc
     7801 gaccaagtgc ttgccgaaca gacccgggtc accggcctgg tcaacacggc gcgcaactcg
     7861 gtcggctgcc aatggctgca gggcggcggc atcctgggtc cgcacttctc gttcacctgg
     7921 tatcgcggca gcccgatcgg ccgcgaacgc aagaccgagg agctgtcgcg ggccagcgtc
     7981 gaggacatca acatcgacgg gcacgagggc ttcatcgcca tcggtgagga cccgacgctc
     8041 ggcaacaact tgtgcgaggt cggcatccag ttccaggacg acttcatcga gtggtcgatc
     8101 agcttttccg agcagccgtt ccctgacccc tgcgatgtgg ccaaggaact gacccgccaa
     8161 tcgattgtga atgcgaaatg agccgctctt tcggccgcag ggcgctggcc ggcctgatcg
     8221 ccgccgtgtc tgtgctgacg ctgctgaccg gctgcaccaa gaccgtcgag ggcaccgccg
     8281 ccaagtcggg atccggtgac gtgcctcgca acgacgactc ggccaagcag taccccaacc
     8341 tgctgaagga atgtgaggtg ctgaccgagg acatcctcgc caagaccgtc ggcgccgatc
     8401 cgctggacat ccagagcacg ttcgtcggcg cggtgtgccg ctggcaggcg gccaacccgg
     8461 cgggcctgat cgacatcacc aggttctggt acgagcaggg cagcctcgac aacgagaaga
     8521 aggtcgccca aagcctgaac tatcagatcg agaaccgctc gatcgcgggc gtgccgtcga
     8581 tcgtgatgaa gcccaacgat ccgaacgggg catgcggggt cgccagcgac gccggcgggg
     8641 tggtcggttg gtgggtgaac ccgcagacgc cgggcatcga cgcgtgcgga
//
LOCUS       DASRJA010000711         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_661690,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000711 DASRJA010000000
VERSION     DASRJA010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661690"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="VFC01_32090"
     CDS             <1..156
                     /locus_tag="VFC01_32090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma"
                     /protein_id="HZN84297.1"
                     /translation="EGIAAETRLGQAGVILVAVDGGGADYRILVRSSFARYLADWLLD
                     AAEEYTL"
     gene            153..2198
                     /locus_tag="VFC01_32095"
     CDS             153..2198
                     /locus_tag="VFC01_32095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HZN84298.1"
                     /translation="MTRHLIDPLMQPFELKNLTLRNRVVSTSHEPSYTEDGMPKARYR
                     LYHEEKAKGGIGLTMMGGSAVVAPDSPAAFGNLHLYKDEIVPWLRELAYGVHEHGTAV
                     MCQITHLGRRTSNYDGDWLPVVYPSPLREPAHRAFPKVAEEWDMDRIVRAYADATARC
                     REAGLDGVEIEAYTHLLDAFWSPLTNHRDDEFGGSLENRMAFPLRVIRAVREAAGPDF
                     AVGIRMSFDEEMPGGLGREEAVAIAHQVVAEGIDFFSIIRGYMASDEALSRVIPPMGT
                     PVAPHLEFAGEIKREVGVPVMHASRINDVATARHAIRDGLLDLVGMTRAHIADPHIVA
                     KIQAGEEDRIRPCVGASLCLDEIYQARDAKCIHNPATGREDRLPHHVAAATGTPRRAV
                     VVGAGPAGLEAARVLGERGHRVVVLEASDAAGGQIRLASSSPRRRDLIGIIDWRLAEC
                     KHFDVDIRYNTYAEADDVLAEDPDVVIVATGGIPNTEFLTEGAQLVSDGWDVLSGAFR
                     VKGDVLLFDDNGQHPGLDAAEVLARAGAKIEFVTPERVLAPDVGGVNYPGYFKVFAEH
                     DVRVTLNERLTAVRRKDGRLEADLYNEYAHVTRQRIVDQVVVEHGTLPSDELYFSLVP
                     DSSNLGEVDQQALLDLRPQEVRRNPDGRYQLFRIGDAVASRNIHAAVFDAYRLCLAL"
     gene            2258..>3254
                     /locus_tag="VFC01_32100"
     CDS             2258..>3254
                     /locus_tag="VFC01_32100"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HZN84299.1"
                     /translation="MPQQVQGVIARSKGAPVELVDITIPDPGPNDVVVRIQACGVCHT
                     DLTYRDGGINDEFPFLLGHEAAGIVESAGSAVTHVQVGDFVVLNWRAVCGNCRACRRG
                     RPWYCFDTFNASQPMTLTDGTELTPALGIGAFAEKTLVHEGQCTKVDPNADPAVVGLL
                     GCGVMAGLGAAMNTGNVSRGDSVAVIGCGGVGDAAIAGARLAGASPIIAIDRDPKKLQ
                     WATELGATHTIDASQADAVEAVQELTDGFGADVVVDAVGRPETWKQAFYARDLAGTVV
                     LVGVPTPDMTLEMPLIDFFSRGGALKSSWYGDCLPERDFPALVDLYEQGRLPLDKF"
BASE COUNT          564 a         1068 c         1086 g          536 t
ORIGIN      
        1 gagggcatcg cggcagagac gaggctcggt caggccggcg tcatcctggt ggcggtcgac
       61 ggcggcggcg ccgactaccg catcctcgtc cgctcgtcgt tcgcccggta cctcgccgat
      121 tggctgctcg acgcagccga ggagtacaca ctatgactcg ccacctcatc gacccgttga
      181 tgcagccctt cgagctgaag aacctcacgc tgcgcaaccg ggtggtcagt acgtcgcacg
      241 agccgtccta caccgaggac gggatgccca aggcgcgata ccgcctttac cacgaggaga
      301 aggcgaaagg cggcatcggc ctgaccatga tgggcgggtc cgcggtcgtg gcgccggaca
      361 gccctgcagc gttcggcaac ctccatctgt acaaggacga gattgtccct tggctgcgtg
      421 aactggccta cggcgttcac gagcacggca cggcagtcat gtgccagatc acccatttgg
      481 gccgcaggac cagcaactac gacggcgact ggctgcccgt ggtctatccc tccccgctcc
      541 gcgaacccgc ccaccgagcc ttcccgaagg tggccgagga atgggacatg gatcgcatcg
      601 ttcgggccta cgccgacgcc accgctcgct gccgcgaagc cggcctggac ggggtcgaaa
      661 ttgaggccta cacccacctg ctcgatgcgt tctggtcgcc gctgaccaat caccgcgacg
      721 acgaattcgg cggcagcctc gagaaccgga tggccttccc gcttcgggtg attcgcgcgg
      781 tccgtgaggc cgcaggcccc gacttcgccg tcggcatccg gatgtccttc gacgaggaga
      841 tgccgggagg actcggccgg gaagaggcgg ttgcgatcgc gcaccaagtc gtcgctgagg
      901 gcatcgactt cttcagcatc atccggggat acatggcaag tgacgaggcg ctgtcccggg
      961 tgattccgcc gatgggcaca cccgttgcgc cgcatctgga gttcgccggc gaaatcaaac
     1021 gcgaggtggg ggtgccggtg atgcacgcct cccgcatcaa cgatgtggcc accgcccggc
     1081 acgccatccg ggacggactg ctcgatctgg tcgggatgac ccgggcgcac atcgccgatc
     1141 cgcacatcgt cgcgaaaatc caagcgggag aagaggaccg gatccgaccg tgcgtgggcg
     1201 ccagcctctg cctggacgag atctaccaag cccgcgacgc caagtgcatc cacaaccctg
     1261 cgacaggccg tgaagaccgg ttgccgcacc acgtggctgc ggccacgggg acaccgcgca
     1321 gggcggtcgt cgtcggagcg gggccggccg ggttggaggc ggcgcgggtc ctcggtgagc
     1381 gggggcatcg cgtggtcgtg ctggaggcca gcgacgccgc aggcggccag atccggctcg
     1441 cgtcctcgtc gccgcggcgc cgcgacctga tcgggatcat cgactggcgg ctggccgagt
     1501 gcaaacactt cgacgtcgat atccggtaca acacctacgc cgaggccgac gacgtgctcg
     1561 ccgaggatcc ggacgtcgtc attgtcgcca ccggtggtat cccgaacacc gaattcctca
     1621 ccgagggagc gcaactcgtt tctgacggct gggacgtgct gagtggcgcc ttccgggtca
     1681 agggcgatgt gctgctcttc gacgacaacg gtcagcaccc tggcctcgat gccgcagagg
     1741 tactcgcacg cgcaggcgcc aagatcgaat tcgtcacgcc ggagcgggtg ttggctcccg
     1801 atgtcggcgg cgtcaactac cccggttact tcaaggtgtt cgccgagcac gacgtgcgcg
     1861 tcacgctcaa cgagcggctg accgcggtgc gccgcaagga cggccggctg gaagccgacc
     1921 tgtacaacga atacgcacac gtcactcggc aacgcatcgt cgatcaggtc gtcgtcgaac
     1981 acggcacgct gccgtcggac gagctgtact tctcgttggt gccagactcg tcgaatctcg
     2041 gtgaggtgga ccagcaagcg ctgctcgatc tacgcccgca agaggtgcga cgcaatccgg
     2101 atggtcgcta tcagctgttc cggatcggag atgcggtcgc cagcaggaat attcacgccg
     2161 cggtgtttga cgcctaccgg ttgtgtctcg cgctgtgaag ctctagcaac cacacatcta
     2221 tcagttccca ttccctacga cgcagtgagg atttttcatg ccacaacagg ttcagggcgt
     2281 catcgcccgc agcaagggcg cgcccgtcga gctcgtcgac atcaccattc cggatcccgg
     2341 cccgaacgat gtggtcgtgc gcatccaggc gtgcggggta tgccacacag atctgactta
     2401 ccgcgacggc ggtatcaacg acgaattccc gttcctgctc ggtcatgagg cggccggcat
     2461 cgtcgagtcg gccggaagcg cggtcacgca cgtccaggtc ggcgacttcg tcgtactgaa
     2521 ctggcgagcg gtgtgcggca actgccgggc gtgccgccga ggccggccat ggtattgctt
     2581 cgacaccttc aatgccagcc agccgatgac gctgaccgac ggcaccgagt tgacaccagc
     2641 gctcggcatt ggcgcgttcg ccgagaaaac gcttgtgcac gaaggacagt gcacgaaggt
     2701 ggatccgaat gccgaccccg ccgtcgtcgg gttgctcggc tgcggtgtga tggccggcct
     2761 gggcgcggcg atgaacaccg gcaacgtctc gcgcggagac tccgtcgcag tgatcggctg
     2821 cggcggggtg ggcgacgcgg cgatcgccgg agcacggctg gctggcgcgt ccccgatcat
     2881 cgcgatcgac cgcgacccca agaagttgca gtgggcaaca gaattgggcg ccacccacac
     2941 aatcgatgcc tcgcaggcgg acgccgtcga ggcagtgcag gagctgaccg acgggttcgg
     3001 tgccgacgtg gtcgtcgacg cggtgggccg cccggaaacc tggaagcagg cgttctacgc
     3061 ccgcgacctg gccggcactg ttgtgctggt aggtgttcca acgccggaca tgacgctgga
     3121 gatgccgctc atcgacttct tctcgcgtgg cggcgcgctg aagtcgtctt ggtacggcga
     3181 ctgcctgccc gagcgtgact tcccggcgct cgtcgatctg tatgagcagg gccgcctgcc
     3241 gctggacaag ttcg
//
LOCUS       DASRJA010000712         7703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1851689,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000712 DASRJA010000000
VERSION     DASRJA010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7703
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851689"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1106)
                     /locus_tag="VFC01_32105"
     CDS             complement(69..1106)
                     /locus_tag="VFC01_32105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZN84300.1"
                     /translation="MIHECVDQYACLAANINFNLEGLRIGFWQLTIDGLSWGAIYALV
                     AVGYTLVFGVLRLINFAHSEIFMLGMFGAYFALDVIMGFTPSGNAYNKGIALTVLYLG
                     IAMLFAMLVSGSAAVGLEFVAYRPLRRRNARSLTFLITAIGASFVMQEFVHFILPHII
                     KGYGGSNAQQPIILVQPKTQFTIFGATVSNVTIVILVAALILALLTDYAINRTKFGRG
                     IRAVAQDSTTATLMGVSRERIIMTTFLIGGLLAGAAALLYTLKVPQGIIYSGGFLLGI
                     KAFSAAVLGGIGNLRGALLGGLLLGIMENYGQAVFGTQWRDVVAFVLLVLVLLFRPTG
                     ILGESLGKARA"
     gene            complement(1205..2419)
                     /locus_tag="VFC01_32110"
     CDS             complement(1205..2419)
                     /locus_tag="VFC01_32110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HZN84301.1"
                     /translation="MRGRVARNVFALGSAGLVVLGIAGCNQSKPEEGGTAPTDLKIVE
                     QVQIDEQGKEIKADQSAAAADPAGDGKATCPPVSIAMAGALNGPDAALGINIKNGVQM
                     AIDKHNAANQGCQVQLKPFDTEGDPQKATAIAPQIVDDQYTIGLVGPAFSGETKATGT
                     VFDQAGLVATTASATNPTLSENGWKTFFRGLANDAVQGPAVANYMKNTLGYKKICVID
                     DSTDYGLGLASAVRTTLGPVADSGCNISVKKGDKDFSAAVTQVKGASPDAVFFGGYYA
                     EAAPLVQQLKDGGVTATFVSADGTKDPQFVSQAGEASKDAILSCPCGPATGTFADEYK
                     KKFNTDAGTYSAEGYDLGTILVKGIDSGAITRPALLDFVKNYDGQGVARKYQWTPTGE
                     LTTNLIWIFKVQ"
     gene            complement(2596..3207)
                     /locus_tag="VFC01_32115"
     CDS             complement(2596..3207)
                     /locus_tag="VFC01_32115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HZN84302.1"
                     /translation="MTGSPTDADKPRRVLVAEDEALIRLDLAEMLREEGYEIVGEAGD
                     GQEAVDLAESLRPDLVIMDVKMPRRDGIDAASEIAGKRIAPIVILTAFSQRDLVERAR
                     DAGAMAYLVKPFNITDLIPAIEVAVSRFSEIAALENEVATLSDRLETRKLVERAKGLL
                     QTKQGMSEPEAFKWIQRAAMDRRTTMKRVAEVVLETLDAPEKA"
     gene            3287..3360
                     /locus_tag="VFC01_32120"
     tRNA            3287..3360
                     /locus_tag="VFC01_32120"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3321..3323,aa:Leu,seq:caa)
     gene            complement(3494..4351)
                     /locus_tag="VFC01_32125"
     CDS             complement(3494..4351)
                     /locus_tag="VFC01_32125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84303.1"
                     /translation="MRTAAISCLVTCGAMVAGPGIAGGAVASADLLGIDLDIFDIFCH
                     NDKKCDHDNDRRGVPGVGSNVPEVRINGIPGTEVPKRGLPGATGGGGGVPRSGGTNRP
                     SNLPPVPTAPGSREIVIRAGPAAPAPAAPAPAAPAPAAPAPAGPAPAGPAPAAPAPAA
                     PVVVPPIVVPPVILSPMVPPAPVIGPVVTPPPPEVHPPSLTPSPRTDRPAPVGEFGPK
                     GIPATFRAGYADYLRAATVGEIAAVAVPGTAGILVITTLGGLVGFRQARAAQALPPAN
                     IANVARFLQ"
     gene            complement(4401..5840)
                     /locus_tag="VFC01_32130"
     CDS             complement(4401..5840)
                     /locus_tag="VFC01_32130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HZN84304.1"
                     /translation="MDIAIVAIYLFGMIAFGFWGKRRATTQSDFLVAGRRLGPMLYSG
                     TMAAIVLGGASTIGGVGLGYKFGISGMWLVFAIGAGILLLSLLFAGRIQRLRVYTVSQ
                     MLELRYGPGSSVLSGVVMWGYTLMLSVTSTIAYSTIFGALFDIGKVPAIILGGGVVII
                     YSTLGGMWSITLTDFVQFLVKTIGIFFILLPIALYKTGGVSGLAEKLPADATSFTAIG
                     GETILTYFVIYTFGLLIGQDIWQRVFTARSPGVARWAGAASGVYCLAYAVAGAVIGMA
                     AKVLLPALESRDDAFAQFVEQQLPPVLAGLVLAAALAAVMSTSSGALIATATVFSQDI
                     VARLRHQDIEAGSEHDHIRNNRLYVFGFGVVMVVIACVLQDVVAALTVAYDILVGGLL
                     VPILGGLVWRRGTNVGAVAAMAVGTVATLATMFFVGDIYANEPIYVGLASGLLVFVAG
                     SLLSKPTDPEVLAEWDRRSRGQEEAPVPV"
     gene            complement(5856..7433)
                     /locus_tag="VFC01_32135"
     CDS             complement(5856..7433)
                     /locus_tag="VFC01_32135"
                     /EC_number="4.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-guanidino-2-oxopentanoate decarboxylase"
                     /protein_id="HZN84305.1"
                     /translation="MTEHRNGGAAVVETLAAHGVDTIFGIPGTHNLEIYRHLPTTGIR
                     AVTPRHEQGAGYAAEAYARVTGRPGVVVTTSGPGLTNVMTAASTAYAESQPMLVLSSG
                     LPTGAEDRDLGQLHEAKNPLGAMDQLVRWSRRVHSADEAAAAVTEAFGSFIGGRPRPV
                     HIEVPFDVLEQQWSGQPRIASKAAPPQADPDAIRRAAEVLSAAGQPMIIAGGGAVDAQ
                     SELTALAEALGAPVATTVNGKGVVDEAHPLSVGASIRLRAVQKAAADSDALLVVGTEL
                     GDSDLWDGQIRGRNVIRCDIDAAQLNKNCPADHSLLGDAAATVAALRATLPAVSQASP
                     ERAATLRAACVQEAMVDAGAYAEINAAVRAALPADGVLTGDSSQVTYFGSVHFFDMPA
                     PRRFCYSPGYATLGYGLPAGLGAAIGRPGTPVAVLLGDGALMFSVQELITLVEQRLPV
                     PVIVVDNGGYQEIKEQEAARGIPPIGVELRTPDLAALAVAMGARGVRTTSTAELTELV
                     GDALGADGPTLIHFDIR"
     gene            complement(7430..>7703)
                     /locus_tag="VFC01_32140"
     CDS             complement(7430..>7703)
                     /locus_tag="VFC01_32140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HZN84306.1"
                     /translation="ERMLARIGDAPVYVSVDIDVLDPAHAPGTGTPEAGGMTSRELLH
                     CLRNLVGVNVVGADIVEVAPAYDHAEITGIAAAHVAYELISVLAKS"
BASE COUNT         1344 a         2557 c         2530 g         1272 t
ORIGIN      
        1 cacccgggac aggccgtccc accactcccg tagacggtcg cccggcgcga gaactttgtc
       61 cgtcacgctc atgcccgtgc cttcccgagg ctctccccga gtatcccggt cgggcggaac
      121 aacagcacca gaacgagcag cacgaacgcc accacgtcgc gccattgggt gccgaacacc
      181 gcctgcccgt agttctccat gatgcccagc agcagcccac cgagcagggc gccgcgcaga
      241 ttgccgatgc cgccgagcac cgcagcggag aatgccttga tacccaacaa gaatccgccc
      301 gaatagatga tgccctgcgg caccttcagg gtgtagagca gcgccgcggc gccggcgagc
      361 agcccgccga tgagaaacgt cgtcatgatg atgcgttcgc gcgacacccc catcagcgtc
      421 gcggtggtcg aatcctgcgc aaccgcccgg atgccgcggc cgaactttgt ccggttgatc
      481 gcgtaatccg taagcaacgc taagattagc gccgccacga ggatgacgat ggtgacgttg
      541 gagaccgtcg caccgaagat ggtgaactgc gtcttgggct gcaccaggat gatcggttgt
      601 tgggcgttgc tgccgccata gcccttgatg atgtgcggca ggatgaagtg cacgaactcc
      661 tgcatcacga acgaggcacc gatcgcggtg atcaggaatg tcagcgagcg ggcgttgcgt
      721 cttcgcagcg gccggtatgc gacgaactcc agcccgaccg ccgccgaacc agacaccagc
      781 atcgcgaaca gcatcgcgat gcccaggtac agcaccgtca gcgcgatgcc cttgttatac
      841 gcgttgccac tcggtgtgaa gcccatgatg acgtcgagcg cgaagtaggc gccgaacatg
      901 cccagcatga agatctcgga gtgggcgaag ttgatcagcc gcagcacacc gaacaccagc
      961 gtgtagccga cggccaccag cgcgtagatc gctccccacg agaggccgtc gatcgtcaac
     1021 tgccagaagc cgattcgcag gccctcgagg ttgaagttga tgttggcggc caggcaggcg
     1081 tactggtcga cgcactcgtg gatcatccgg tgagcggctc cttgcgattg tgcttttgca
     1141 aagacgtgtc tttagcgaaa acgcgtccga gcctttcggt ctcggacgcg cctcgcttca
     1201 gcggttactg aaccttgaag atccagatca ggttggtggt gagctcaccg gtgggggtcc
     1261 actggtactt acgcgccaca ccctgaccgt cgtagttctt cacgaagtca agcaacgcgg
     1321 gccgggtgat ggcgcccgaa tcgatgccct tgaccaggat ggtgcccagg tcatatccct
     1381 ctgcgctgta ggtgcccgca tcggtgttga acttcttctt gtactcatcg gcgaacgtcc
     1441 cggtggccgg accgcacggg caggacaaga tcgcgtcctt agatgcttca ccggcctggc
     1501 tgacgaactg cggatccttc gtgccgtcgg cggacacgaa cgttgccgtc acgccaccgt
     1561 ccttgagctg ctggacgagc ggcgcggctt cggcgtagta cccgccgaag aagacggcgt
     1621 ccgggctggc gcccttgacc tgggtcaccg cagcggagaa gtccttgtca cccttcttca
     1681 ccgagatgtt gcaccccgaa tcggccaccg ggcccagcgt cgtgcgcacc gcagaggcga
     1741 ggccaagccc gtagtcggtg ctgtcgtcga tcacgcagat cttcttgtag cccagggtgt
     1801 tcttcatgta gttggcgacc gccgggccct gaacggcatc gttggccagg ccgcggaaga
     1861 acgtcttcca gccgttctcg ctgagtgtgg ggttcgtcgc cgacgcggtg gtggcgacaa
     1921 gaccggcctg atcgaatacg gtgccggtgg ccttggtctc gccggagaac gccgggccga
     1981 cgaggccgat cgtgtactga tcgtcgacga tctgcggggc gatcgcggtg gccttctgcg
     2041 gatcaccctc ggtgtcgaac ggcttcagct gcacctggca gccctggttg gcggcgttgt
     2101 gcttgtcgat ggccatttgg acgccgtttt tgatgttgat gccaagcgcg gcgtcgggcc
     2161 cgttgagcgc gcccgccatc gcgatcgaca ccggcgggca ggtggccttg ccgtccccgg
     2221 cgggatcggc ggcagcggca ctctggtcgg ccttgatctc cttgccctgc tcgtcgatct
     2281 gcacctgctc gacgatcttc agatcggtcg gcgccgtgcc cccttcttcc ggcttggact
     2341 ggttgcagcc ggcaatgccg agcacaacca aacccgcact accgagagca aatacattcc
     2401 gtgccacgcg accgcgcacg cttcacctcc gggtcaatgt tttcatcggc atcgacgcac
     2461 cagcccaggt tgagtggggc ggcgatgctt cgggacgacc atagccaacg catcgccgtg
     2521 tcgcgtgatg ttggaaaacg cgcaccgccg accggctgct aaatcgttac caacggcgcg
     2581 tcggaaacgc agtatttacg ctttttccgg cgcatcgagg gtttccagca ccacctcggc
     2641 gactcgtttc atcgtggtcc ggcggtccat cgctgcgcgc tgaatccact tgaacgcctc
     2701 gggctcgctc atcccctgct tggtctgcag caaacccttg gcgcgctcga ccaacttgcg
     2761 cgtctccaat cggtccgaca gtgtcgcgac ttcgttttcc agggcggcga tctcgctgaa
     2821 ccggctcacc gccacctcga tcgcggggat gaggtcggtg atgttgaacg gcttgacgag
     2881 atacgccatc gcgcccgcgt cacgggcgcg ctccaccagg tcgcgctggc tgaacgcggt
     2941 caggatgacg atcggcgcga tccgcttgcc ggcaatttcc gacgcggcgt cgatgccatc
     3001 gcgacgcggc atcttgacgt ccatgatcac cagatccggt cgtaggctct cggccagatc
     3061 gaccgcctcc tgcccatcgc cggcctcgcc gacgatctcg taaccttctt cgcgcagcat
     3121 ctcggccaag tccagccgga tgagcgcctc gtcttcggcg acgagaacac ggcgcggctt
     3181 gtcggcgtcg gtcggtgacc cggtcatggg tcacattgtg gcggtcgcac gcccgatcag
     3241 cgaccgcggg gcatcctcta tggtgggaag ccgcgagaaa caccacgccc tcgtatccca
     3301 attggcagag gaactggatt caaaacccag acagtgtgag ttcgagtctc accgagggca
     3361 caagcgattg agccgagcga cacgggagct cgctcacgcg tccgaggcga ttgagcttgc
     3421 tgacgagctg gttcgcagaa ccagcgagga acacaagcga gcgggttcga agagcgcgcg
     3481 acgaacacca acgtcattgc aggaatcgcg caacattcgc gatattcgcg ggcggcagcg
     3541 cctgcgctgc gcgagcctgc cggaacccca ccaggcctcc cagcgtggtt atcaccaata
     3601 tgcccgctgt acccggcacg gccacggccg caatttcgcc taccgtcgcg gctcttaggt
     3661 agtcggcgta cccggcgcgg aatgttgccg gtatcccttt ggggccgaac tcaccaactg
     3721 gcgccggacg gtcggttcgc ggggacgggg tcagcgaggg cgggtgcacc tccggcggcg
     3781 gcggggtcac caccggaccg atgacgggtg cgggcggcac catcggcgaa aggatcaccg
     3841 gcggaacgac gatcggcgga acgaccaccg gcgccgcggg agccggggcc gcgggagccg
     3901 gacccgcggg agccggaccc gcgggtgccg gagccgcggg tgccggggcg gcgggagccg
     3961 gggcggcggg agccggcgca gcgggccccg cccggattac gatttcgcgc gaacctggcg
     4021 cggtcggcac cggcggcagg ttcgacggcc tgttcgtgcc gccgctgcgg gggacgccgc
     4081 cgccacctcc agtcgcacct ggaagaccgc gctttggtac ttcggtacca ggtatcccgt
     4141 tgatgcggac ctcgggcacg ttgctaccaa ccccgggaac gccacggcga tcgttgtcgt
     4201 ggtcgcactt cttatcgttg tggcagaaga tatcgaagat gtcgaggtcg atgccaagca
     4261 ggtcggccga tgccaccgca ccaccagcga taccgggacc cgcgaccatc gctccgcagg
     4321 tcaccaggca gctgatcgcc gctgtgcgca tccacgacag tctcatctca atggcattct
     4381 tgcacgcccg cagagtttgc tcacaccgga acaggtgctt cttcctgtcc gcggctgcgt
     4441 cgatcccatt cggccagcac ctcgggatcg gtcggcttgc tgagcaggct tcccgccacg
     4501 aacaccagca ggcccgacgc gagcccgacg tagatcggct cgttggcgta gatgtcgccc
     4561 acgaagaaca tcgtggccag ggtggcgacg gtgccgacgg ccatcgccgc caccgccccg
     4621 acattggtgc cgcgccgcca gaccagaccg cccaggatcg gcaccagcag gccgccgacc
     4681 aggatgtcgt aggccacggt cagcgcggcg accacatcct gcaagacgca cgcgatgacc
     4741 accatgacga caccgaatcc gaagacatag agccggttgt tgcggatgtg gtcgtgctcg
     4801 ctacccgcct cgatgtcttg gtgacgcagc cgggcgacga tgtcctgact gaacacggtc
     4861 gcggtggcga ttagcgcgcc gctcgacgtc gacatcaccg cggcgagcgc agcagccagc
     4921 acgaggccag ccagcacagg tggcagctgc tgctcgacga actgcgcaaa ggcgtcgtct
     4981 cgggactcca gcgcgggtag cagtaccttg gccgccatcc cgatcaccgc accggccacc
     5041 gcgtaggcca ggcagtacac accggacgcc gcgccggccc agcgggccac tcccggactg
     5101 cgcgcggtga acacgcgctg ccagatgtcc tggccgatca gcaggccgaa cgtgtagatg
     5161 acgaagtagg tcaggatcgt ctcgccgccg atcgcggtga acgacgtggc gtcagctgga
     5221 agcttctcgg ccagaccgga aacgccaccg gtcttgtaca gcgcgatcgg cagcaggatg
     5281 aagaagatgc cgatggtttt gacgaggaac tgcacgaaat cggtgagcgt gatcgaccac
     5341 atgcccccca gcgtggagta gatgattacc acgccgccgc cgaggatgat cgccggcacc
     5401 ttgccgatgt cgaacagcgc gccgaagatc gtcgaatagg caatcgtcga agtgaccgac
     5461 agcatcagcg tgtagcccca catcaccacg cccgacagca cggaagagcc agggccgtaa
     5521 cgcaattcga gcatctggct gacggtgtag acgcggagcc gctggatccg gcccgcgaac
     5581 agcaggctca gcagcaggat gcccgcgccg atcgcgaaca ccagccacat gcccgagatg
     5641 ccgaatttat agccaaggcc gaccccgccg attgtcgagg cgccaccgag cacgattgcg
     5701 gccatggtgc ctgaatacag catcgggccg agtcggcggc cagccaccag aaagtcggat
     5761 tgcgttgtgg ctctgcgctt tccccagaag ccaaacgcga tcattccaaa cagatagatg
     5821 gcgacaatcg ctatgtccat gagtctggtg tcctttcagc ggatgtcgaa atggatgagg
     5881 gtcgggccgt cggcgccgag agcgtcgccg accagttcgg ttagttcggc cgtcgaggtg
     5941 gtacgcaccc cgcgcgcgcc catagcgacc gccagggcgg ccagatccgg ggtgcgtagt
     6001 tcgaccccga tcggtgggat gccgcgtgcg gcttcctgtt ctttgatctc ctgatagccg
     6061 ccgttgtcta cgacgatcac cggtaccgga agccgttgct ccacaagcgt tatcagctcc
     6121 tgaacggaga acatcagtgc gccgtcaccg agcagcacgg ctaccggggt cccaggccgg
     6181 ccgattgcgg cgccgagtcc ggcgggcagc ccgtaaccca gggtggcgta gccgggtgag
     6241 tagcagaacc ggcgcggtgc gggcatgtcg aagaaatgca ccgagccgaa ataggtgacc
     6301 tgcgaactgt ccccggtgag cacgccgtcg gcgggcagcg ccgcgcggac cgcggcgttg
     6361 atctcggcgt aggcccccgc gtcgaccatc gcctcctgga cgcatgctgc ccgcagcgtc
     6421 gcggcccgct caggcgaggc ctgcgagaca gcgggcagcg tggcgcgaag tgcggcaacg
     6481 gtggcggcgg cgtcgcccag cagagaatgg tcggccgggc agttcttgtt cagttgggca
     6541 gcatcgatgt cgcagcggat cacgttccgg ccgcgaatct ggccgtccca caagtcggag
     6601 tcgccaagct cggtgcccac caccagcagc gcgtcgctgt cggcggcggc cttctgcacg
     6661 gcgcgcagcc ggatcgacgc cccgaccgac agcggatgcg cctcgtcgac gacgcccttg
     6721 ccgttgacgg tggtggcgac gggcgcgccg agcgcctccg cgagtgccgt cagctcgctc
     6781 tgagcgtcga cggcgccgcc gccggcaatg atcatcggct ggccggcggc cgacagcacc
     6841 tcggccgccc gtcgtatcgc gtccggatcg gcttggggag gagcggcttt cgaggcgatc
     6901 cgtggttggc ccgaccattg ctgctcgagc acgtcgaagg gaacctcgat gtgaacgggc
     6961 cgaggacggc cgccaatgaa gctgccgaac gcctcggtca ccgcggcggc cgcctcgtcg
     7021 gcgctgtgca cccgtcggct ccagcgcacc agctgatcca ttgcgcccaa ggggttcttg
     7081 gcctcgtgca gctggccgag gtcacggtcc tcggcgccgg tgggcaggcc cgacgacagc
     7141 accagcatcg gctgtgattc cgcgtaggcc gtcgacgccg cagtcattac gttggtcagc
     7201 cctgggccgc tggtggtcac cacgacaccc ggccgtccgg tgactcgtgc atacgcctcg
     7261 gcggcgtaac cggcaccctg ctcgtggcgc ggggtgaccg cccggatccc ggtcgtcggc
     7321 aggtggcgat aaatctccag gttgtgtgtg cccgggatgc cgaagatggt gtcgacgcca
     7381 tgtgcggcaa gggtttccac gacggccgca ccgccgttgc gatgctcggt catgacttcg
     7441 ccagcacgga gatcagctcg tacgccacgt gcgcggcggc gatgccggtg atctcggcgt
     7501 ggtcgtacgc cggggcgacc tccacgatgt ccgcgccgac gacgttgacg cccaccaggt
     7561 ttcgcaagca gtgcaacaac tcgcggctgg tcatcccgcc ggcttcgggg gtaccggtgc
     7621 cgggggcgtg tgccgggtcg agcacgtcga tgtcgacgga gacgtagacc ggggcgtcgc
     7681 cgatacgggc cagcatccgc tcg
//
LOCUS       DASRJA010000713         3247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1467694,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000713 DASRJA010000000
VERSION     DASRJA010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467694"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..642)
                     /locus_tag="VFC01_32145"
     CDS             complement(223..642)
                     /locus_tag="VFC01_32145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZN84307.1"
                     /translation="MPAANADNKRLNDGVVANVYTIQHQAGCTNNVTISPQLQLAAEW
                     HANDVLNNRALDGDIGSDGSTPQSRADAAGFKGAVAETVAINPALAISGIEILNQWYY
                     RPDYMAIMSNCANSKMGVWSVNSLDRTVVVAVYGQPA"
     gene            complement(716..2230)
                     /locus_tag="VFC01_32150"
     CDS             complement(716..2230)
                     /locus_tag="VFC01_32150"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZN84308.1"
                     /translation="MVEFSGHHARFPTITNQLYQLAAGTVTSDQLVRRSLDAIDASQP
                     TLNAFRVVLRERALADAAAADRKRAAGKDLPLLGIPIAVKDDVDVAGVPTRFGADGHI
                     REAQQDSEVVRRLRDAGAVIVGKTNTCELGQWPFTSGPAFGHTRNPWSSEHTPGGSSG
                     GSAAAVAAGLVAAAIGSDGAGSVRIPAAWTHLVGIKPQRGRISTWPLPEAFNGITVNG
                     VLARTVTDAALVLDAASGNVPGDLHQPPPVRVSDFVAQAPGPLKIAMSTKFPYAVFKA
                     KLHPEIRAAMETVAGQLEELGHTVVRADPDYNLRMSWNFLSRSTSGLLDWVDRLGPNV
                     NLDKRTLANTRTGWLLSQNTLRKARAREAQSQRRIGWTFNLFDVVLAPTTALPPPRVT
                     DFDHRGGLSTDRAMIRACPVTWPWNLLGWPSINVPAGFTYGGLPIGVQLMGPANSEPL
                     LISLAAELEALNGWVMRQPDVWWDRRDRQPAPELVSEAALENLAADEPVPGEVA"
     gene            2294..3139
                     /gene="recO"
                     /locus_tag="VFC01_32155"
     CDS             2294..3139
                     /gene="recO"
                     /locus_tag="VFC01_32155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZN84309.1"
                     /translation="MRLYRDRAVVLRQHKLGEADRIVTLLTRDHGLVRAVAKGVRRTR
                     SKFGARLEPFAHIDVQLHPGRNLDIVTQVQAIDAFATDIVSDYGRYTSACAVLETAER
                     LAGEERAPVPALHRLTVAALRAVADGSRPRELVLDAYLLRAMGIAGWAPALTECARCA
                     APGPHRAFHVAAGGSVCVHCRPSGSATPPQGVLDLMAALNDGDWEHAQESPPSYRSQA
                     SGLVAAHLQWHLERQLRTLPLVERAYRVDRTVAEQRVSVFGQDVHRGDETGGQDHLPT
                     AASGA"
     gene            3090..>3247
                     /locus_tag="VFC01_32160"
     CDS             3090..>3247
                     /locus_tag="VFC01_32160"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969879.1"
                     /note="catalyzes the formation of undecaprenyl
                     pyrophosphate from isopentenyl pyrophosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HZN84310.1"
                     /translation="MKRAAKTTYPQLPPAPDDYPTFPDTSTWPVVFPDLPRRTNGRFA
                     RPPQHTSKA"
BASE COUNT          512 a         1081 c         1106 g          548 t
ORIGIN      
        1 agtgtggtaa gtgcggtgcg gatcacgggg gtaccttcgt tcgttcgggt tgcctttccg
       61 gcgatttgga atgacggtat gggcccggga ctggttgcag aatggtgccc aaaccccctt
      121 ctgatcgccc tcgtgcgggt actgcgcggc agatccgttt aggggtttag gggttagcaa
      181 ccgttacgga taaccctcgt tgagtgcaca ctcgacgaac gtctaggccg gctggccgta
      241 aaccgcgacg acgaccgtgc ggtccaggct gttcaccgac cacacaccca tcttcgagtt
      301 ggcgcagttc gacatgatcg ccatgtagtc cggccggtag taccactggt tcaggatctc
      361 gatgccgctg atcgcgagtg cggggttgat ggcgacggtt tcggcgacag cacccttgaa
      421 cccggcggcg tcggcgcgac tctgtggcgt cgacccgtcg gatccgatgt cgccgtcgag
      481 cgcacggttg ttcagcacgt cgttcgcgtg ccactccgca gccagctgca actgcggact
      541 gatcgtcacg ttgttggtac agcctgcttg atgctggatg gtgtagacgt tggcgacgac
      601 gccgtcgttg agccgcttgt tgtcggcgtt ggcggcgggc atacccgcaa ccgccgcgcc
      661 gatcaccgcg acaaccggta gcacgcggca cgccacaacc ttcacgctgg ggagattacg
      721 cgacctcgcc gggcaccggc tcgtcagcgg caagattctc cagcgcggcc tcggaaacca
      781 gttccggcgc cggctggcgg tcgcgacgat cccaccagac gtcgggctgc cgcatgaccc
      841 aaccattgag cgcttccagt tccgcggcca gtgagatcag cagcggctcg ctgttggccg
      901 ggcccatcag ctgaacgccg atcggcagcc cgccgtaggt gaacccggcg ggcacgttga
      961 tcgacggcca tccgagtagg ttccaaggcc aggtcaccgg gcatgcgcgg atcatggcgc
     1021 ggtcggtcga cagcccgccg cggtgatcga agtcggtcac cctgggcggc ggcaacgcgg
     1081 tcgtcggcgc caggacaacg tcgaaaagat tgaacgtcca cccgatccgt cgctgcgatt
     1141 gcgcttcacg ggcgcgggcc ttgcgcaacg tgttctgcga cagcagccac ccggtccgcg
     1201 tattagccag cgtccgcttg tccaggttta cgttcgggcc gaggcgatcc acccaatcga
     1261 gcaacccgga cgtcgaccgg gacaggaagt tccacgacat ccgcaggttg tagtcggggt
     1321 cggcccgcac gacggtgtgc ccgagctcct cgagctgacc cgcaacggtc tccatcgccg
     1381 cccggatctc cggatgcagc ttggccttga agactgcgta cgggaacttc gtcgacatcg
     1441 cgatcttcag cgggccggga gcctgcgcga cgaaatccga cacccgcacc ggcggcggct
     1501 gatgcaggtc accgggcacg ttgcccgatg ccgcgtccag caccagcgcc gcatccgtca
     1561 ccgttcgggc caacaccccg ttgacggtga tgccgttgaa cgcctcgggc agcggccagg
     1621 tggagatccg tccgcgctgc ggtttgatgc cgaccagatg cgtccacgcc gcgggaattc
     1681 gcacactgcc cgcgccgtcc gagccgatgg ccgccgcgac caggccagcc gccacggccg
     1741 cggcgctgcc acccgacgaa ccaccaggcg tgtgttcgct cgaccacggg ttgcgggtgt
     1801 gcccgaacgc cgggccgctg gtaaacggcc actgacccaa ctcgcaggtg ttggtcttgc
     1861 cgacgatgac cgcccccgcg tcgcgcagtc gacggaccac ttcggagtcc tgctgcgcct
     1921 cccggatgtg gccgtcggca ccgaaccggg tgggcacgcc ggcgacgtcg acgtcgtctt
     1981 tgacggcgat cggtattcct agcaacggaa ggtctttgcc cgccgcacgt ttgcggtcgg
     2041 ccgccgccgc gtcggcgagc gcgcgctctc gcaatacgac gcggaacgcg ttgagcgtcg
     2101 gctggctcgc atcgatggca tcgagtgatc gccgtaccag ttgatccgac gtcacggtgc
     2161 cggctgcgag ctgataaagc tggttggtga tagtaggaaa tcgggcgtgg tgtccggaaa
     2221 actcgaccat cggttacgag aatatcggcg ctgcatccca gcaactgtca gatcacggtg
     2281 agagactttc ttaatgcggc tgtatcggga ccgggcggtg gtgctgcgcc agcacaagct
     2341 cggcgaggcc gaccggatcg tcaccctgct cacccgcgac cacgggttgg tgcgcgcggt
     2401 ggccaagggg gtgcgacgca cacgcagcaa gttcggtgcg cggctggagc cgttcgcgca
     2461 catcgacgtt cagctgcatc ctggccggaa cctcgacatc gtcacccagg tgcaggccat
     2521 cgacgcgttc gccaccgaca tcgtcagcga ctacggccgc tataccagtg catgcgccgt
     2581 gttggagacc gcggagcggc tggcagggga ggagcgggcc ccggtgcccg cgctgcatcg
     2641 gctcacggtg gccgcgttgc gggcggtggc cgatggcagc cgtccccgcg agctcgtgct
     2701 ggacgcttat ttgttgcgcg ccatgggaat cgcgggctgg gcgccggcgc tgaccgaatg
     2761 cgcccgctgc gccgcgccgg gtccgcaccg ggcgttccac gtcgcggccg gaggaagtgt
     2821 gtgcgtgcac tgccgcccgt ccgggtcggc gaccccgccg cagggggtgc tggacctgat
     2881 ggccgcgctc aacgacggcg actgggagca cgcgcaggag tcgccgccgt cgtatcgcag
     2941 ccaggccagc gggctggtgg ccgcgcatct gcagtggcat ctggaacgtc agctgcgaac
     3001 attgccgctg gtcgagcggg cctaccgggt cgatcgcacg gtcgctgagc agcgtgtgtc
     3061 ggtgttcggg caggatgtgc accgtggtga tgaaacgggc ggccaagacc acctacccac
     3121 agctgcctcc ggcgcctgac gactatccga cgtttcccga tacgtcgacg tggccggtgg
     3181 tgttcccgga cctgccgcgg cggaccaacg gcaggttcgc ccgccccccg cagcacacct
     3241 cgaaggc
//
LOCUS       DASRJA010000714         6643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_970387,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000714 DASRJA010000000
VERSION     DASRJA010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970387"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VFC01_32165"
     CDS             <1..859
                     /locus_tag="VFC01_32165"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308964.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HZN84311.1"
                     /translation="DERYARASEYMDVVTKLWESWEPDAVERNTTTGTFANFKKVHTI
                     DFDGKYYKVRGPLNTAPSPQYRPTIAQAGASPPGRELAARHADTIVAPANGIESMRSY
                     REDIHARMKAHGRDPSECKLLFLVSPIVADTHDEAQAKAQRWFTDPLYIEYMLAEISS
                     ITEIDFAQFDLDEPLPELSTNGERGALQSFVSRGKDKTLRELVTNTGVIGGNLQFCGT
                     PAEVADEMGEVMKQVGGDGFLITSPVMRLNRRYVTEITDGLVPELQRRGLVRKEYTTT
                     MLRDHLREF"
     gene            922..2100
                     /locus_tag="VFC01_32170"
     CDS             922..2100
                     /locus_tag="VFC01_32170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN84312.1"
                     /translation="MTDHNTTKVVVIGGGYSGTMAANHLRMRGDVDITLVNPRPKFVE
                     RIRLHQFVAGNYDATVDYGTLLGEGIKLVVDAATRIDTAFRTVELASGRALDYDYVIY
                     AVGSTAVAPASVPGAAEFAHPVGELEYAHWLRDSLDELHPDAPVTVVGAGLTGTETAA
                     ELAEAGRRVTLVCGGALVPSLSAAGRRSVAKWLAKHGVAVLENDVVTEVRADAVVFAD
                     GAVRPSALTIWTAGFGVPDLAVQSGLRTDGIGRLLTDETLTSVDDDRIVASGDAAAPS
                     GVPLRMSCQAAVPLGAQAANTVLSRIAGTAPNTVDIAFVGSNVSLGRRAATIQWAHKD
                     DTPTKFYTGGRLGGAIKEAVCKGTLWGIRREASKPGSVFWFKGGKRPSQPALEMVAES
                     "
     gene            2094..2996
                     /locus_tag="VFC01_32175"
     CDS             2094..2996
                     /locus_tag="VFC01_32175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HZN84313.1"
                     /translation="MVTVTDEHAERFTHLRPLLFTIVYEILGSATESDDVLQDSYLRW
                     ADVDLATVRDTKSYLAQLVTRQALNALRAGARRREDYIGPWLPEPLLLDDRDASADVV
                     LAESVSMAMLVLLETLTPDERAVFVLREVFGFDYDEIAGAVGKSATTVRQVAHRAREH
                     VHARRKRFEPVDTDTTTKLTEQFMTAAATGDMEGLLAMLAPNATWTADSGGKATAARR
                     PIVGAEKVARAIMGLFRYGQRMPDMRFEMVNCNNAPAVVVYTGDHLEGVFLVEIIDGK
                     ITQFYAMRNPDKLTAIAVPREISR"
     gene            3017..4267
                     /locus_tag="VFC01_32180"
     CDS             3017..4267
                     /locus_tag="VFC01_32180"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HZN84314.1"
                     /translation="MRIERLGDLGSAPAVLRAVAYAGARLGLPPPAALIGEWFDSGAV
                     IAPSLKAVAVEDVFAVPAGESGDAVGGGWFGYLSYPDAGVDGRGPRIPEAAGGWSDHV
                     LRQDRDGQWWYESLSGAPLSAWVTEALRSRVPAREHGIAWGDADRDAHRRGVLECLEA
                     IAAGEIYQACVCTQFKGRLDGAPIDFFVEAVERTTPARAAYVAGSWGAVASLSPELFL
                     QRSGETVTSSPIKGTLPRHADPLALRASVKDVAENIMIVDLVRNDLGRVALTGTVTVP
                     ELLVVRPAPGVWHLVSTVSARVGVGVPMGSVLDATFPPASVTGTPKLRARQLLRGWEP
                     HRRGVYCGTVGLASPVAGCELNVAIRTVEFDASGNAVLGVGGGITADSDPDREWDECL
                     HKAAPFIDIADSAQRVSRPTVSAR"
     gene            complement(4209..4946)
                     /locus_tag="VFC01_32185"
     CDS             complement(4209..4946)
                     /locus_tag="VFC01_32185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN84315.1"
                     /translation="MALPALVLVHGGGHAADCWEPTIDELHRQAPELTVLAVDLPGRR
                     GKPGDLYTLTIADFVESVVADIEEAGLGDIVLAGHSMAGLTVPGVLTKVGSSRVREMI
                     LATAFVPPEGKALADTLTGLFKRIARRAASSGKLTPTPRAMARFGFFNGMTRAQRRFM
                     VGKLYLEPPGILAENVSRRDMPDDVPRTWILTTRDRALSQKSQRRSIAAIGGVQTIIE
                     MDTCHDLMVSEPKRLAEILVERCRLYQ"
     gene            complement(4959..5795)
                     /gene="rsmI"
                     /locus_tag="VFC01_32190"
     CDS             complement(4959..5795)
                     /gene="rsmI"
                     /locus_tag="VFC01_32190"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZN84316.1"
                     /translation="MTTGRLLIGATPLGQPSDASARLVKALASADVIAAEDTRRVRNL
                     AQALEVKPAGKVVSLYDANEATRVPALVDEIQAGATVLLVSDAGMPLISDPGYRLVTA
                     CIEAGAPVHCLPGPSAVTTALAVAGLPSERFCFEGFAPRKHSARLAWLKTLAAERRTC
                     VFFESPRRLADTLKDAVDVLGADRKAVVCRELTKTHEEIVRGTLGELAEWANDKVLGE
                     ITVVLAGATPKADLATLIDEVNALVDDGVRVKDACTQVVEANPGAPSRRELYDAVLRS
                     RQ"
     gene            5820..>6643
                     /locus_tag="VFC01_32195"
     CDS             5820..>6643
                     /locus_tag="VFC01_32195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HZN84317.1"
                     /translation="MTAPADELVAKGRAVPVISPAPLVPVADFGPVDRLQGWAMTAVV
                     TALAAVTRFLNLGSPTDAGTPIFDEKHYAPQAWQLLRNHGVEDNPGYGLVVHPPVGKQ
                     LIAIGEALFGYNGLGWRFSGAVCGVIVVLLVVRIGRRISRSTVVGGIAGLLLIADGVS
                     FVASRTALLDGFLELFVVAAFGCLMVDRDQVRERMHIALLEGRIGETPWGPRLGVRWW
                     RFGAGVCLGLACATKWSGLYFIVFFGFMTLAFDIAARRQYRVPRPWVGALRRDLGPS"
BASE COUNT         1028 a         2165 c         2337 g         1113 t
ORIGIN      
        1 cgacgagcgg tacgcgaggg ccagcgagta catggacgtg gtgaccaagt tgtgggagtc
       61 gtgggagccc gacgcggtcg aacgcaacac caccacaggc acattcgcga acttcaagaa
      121 ggtccacacc atcgacttcg atggcaagta ctacaaggtg cgtggcccgc tgaacaccgc
      181 gccgtcaccg cagtaccgtc cgaccatcgc gcaggccggc gcgtccccac ccggacgtga
      241 gctggcagcg cggcacgccg acacgatcgt cgcgcccgcc aacggcatcg agtcgatgcg
      301 gtcctatcgc gaggacatcc atgcccgcat gaaggcccat ggccgcgacc cgtcggaatg
      361 caagctgttg ttcctggtgt ccccgatcgt ggccgacacc cacgacgagg cacaggccaa
      421 ggcgcagcgg tggttcaccg atccgctcta catcgagtac atgctggccg agatctcgtc
      481 gatcaccgag atcgacttcg cccagttcga tctcgacgag cccctgcctg agttgtcgac
      541 gaacggcgag cggggcgccc tgcagagctt cgtttcccgc ggcaaggaca agacacttcg
      601 cgagttggtc accaacaccg gagtcatcgg gggcaaccta cagttctgcg gtacacctgc
      661 cgaagtggcc gacgagatgg gtgaggtgat gaaacaggtt ggcggcgatg gcttcttgat
      721 caccagcccc gtcatgcggc tcaaccgccg ctacgtcacc gagatcaccg acggcctcgt
      781 tcccgagttg cagcgacgcg gtctggttcg caaggagtac acgaccacga tgctgcgcga
      841 ccacctgcgc gagttctgac atggctgtgt gacgcgcgtc acgtcctgtc acatctcagc
      901 gatcagcggt gtcttcaggg catgactgat cacaacacca ccaaggtcgt cgtcatcgga
      961 ggcggctact ccgggacgat ggcggccaac catctccgca tgcggggcga cgtcgacatc
     1021 accctggtca acccgcggcc gaagttcgtc gagcggatcc ggctgcatca gttcgtggcc
     1081 ggcaactacg acgcgacggt cgactacggc acgctgcttg gcgagggcat caagctggtc
     1141 gtcgacgccg cgacgcgcat cgacaccgcc ttccgcaccg tggagctggc gtcgggccgt
     1201 gccctggact atgactacgt catctacgcg gtcggcagca ccgcagtggc accggcttcg
     1261 gtgcccggcg ccgccgaatt cgcccatccc gttggggaac tggagtacgc gcactggctg
     1321 cgggacagcc tcgacgagct ccatcccgac gctccggtga ccgtcgtcgg cgccgggttg
     1381 accggcaccg agaccgccgc tgagttggcc gaagcgggcc gccgggtaac gctggtgtgt
     1441 ggcggggcgc tcgttccgtc gctgagtgca gcgggccgcc ggtccgtggc caagtggctg
     1501 gccaagcacg gcgtcgccgt gctggagaat gacgtcgtga ccgaggtgcg cgcggatgcg
     1561 gtggtgttcg ccgatggtgc ggtgcggccg agcgcgctca cgatttggac ggccggattc
     1621 ggtgtgcccg acctagccgt tcagagcggt ttgcgcaccg acgggatcgg ccggctgctc
     1681 accgacgaga cgctgaccag cgtcgacgac gatcgcatcg tggcgagcgg ggacgccgcc
     1741 gcgccgtcgg gtgtgccgct gcgaatgagc tgccaggccg cggttccgct cggggcgcag
     1801 gcagccaaca ccgtgctcag ccgcatcgcg ggcaccgcgc cgaacacggt ggacatcgcg
     1861 ttcgtgggct ccaacgtcag ccttggccgg cgcgccgcca ccatccagtg ggcccacaag
     1921 gacgacaccc cgacgaagtt ctacaccggc ggccggcttg gcggagccat caaggaagcg
     1981 gtctgcaagg gcaccctgtg gggtatccgc cgcgaggcca gcaagcccgg ctcggtgttc
     2041 tggttcaagg gcggcaagcg tccgtcgcag ccggcgctcg agatggtcgc ggaatcgtga
     2101 cggttaccga cgaacacgcc gagcggttca cccacctaag gccgctgctg ttcacgatcg
     2161 tctacgaaat ccttggttcc gcaacggaat ccgacgacgt gctgcaggac agctatctgc
     2221 ggtgggccga cgtcgacctc gcgacggtac gcgacaccaa gtcgtatctc gcgcaactgg
     2281 tgacccgcca agcgctcaac gcgcttcggg cgggcgccag gcgtcgcgag gactacatcg
     2341 ggccgtggct gcccgagccg ctgttgctcg acgaccgcga cgcctccgcc gatgtggtgc
     2401 tggcggagtc ggtgtcgatg gcgatgctcg tgctgcttga aacgctcact cccgacgaac
     2461 gggcggtgtt cgtgctgcgt gaggtgttcg gcttcgatta cgacgaaatc gccggggcgg
     2521 taggcaaatc cgcgacgacg gtgcgtcaag tggcgcaccg cgccagggag catgttcatg
     2581 cgcggcgcaa gcgattcgag cccgtagaca ccgacaccac aacgaagctc accgagcaat
     2641 tcatgactgc ggcagcgacc ggagacatgg aagggctgct ggcgatgctg gcgcccaatg
     2701 cgacgtggac ggccgacagc ggcggcaagg cgaccgcggc ccgccggccg atcgtgggtg
     2761 cggagaaggt cgccagggcg atcatgggac tgttccgcta tgggcagcgc atgccggaca
     2821 tgcggttcga gatggtgaac tgcaacaacg cccctgcggt cgtggtctac accggagacc
     2881 acttggaagg cgtcttcctg gtcgagatca tcgacggcaa gatcacgcag ttctacgcga
     2941 tgcgcaaccc ggacaaattg acggcgatcg ccgtcccgcg ggagatcagc cgctagcgct
     3001 ctgtcacgct tggtcggtgc ggatcgagcg gctcggcgat ttggggagcg cccctgcggt
     3061 gctgcgggcc gtcgcctacg ccggcgccag gctcgggttg cctccgcccg ccgcgctgat
     3121 cggtgagtgg ttcgactcgg gggcggtcat cgccccgtca ctgaaggccg tcgccgtcga
     3181 ggacgtcttc gccgtgcccg cgggggaatc gggtgacgct gtcggcggag gatggttcgg
     3241 ctacctgtcg tacccggacg cgggcgtcga cggtcgcggt ccacggatcc cggaggcggc
     3301 cggcggctgg tccgaccacg tgctgcgtca ggaccgcgac gggcagtggt ggtatgaaag
     3361 tctctccggt gcaccacttt ccgcgtgggt gaccgaggcg ttgcgttcgc gagttcctgc
     3421 ccgcgagcac ggcatcgcgt ggggcgacgc cgaccgagat gcgcaccgcc gcggtgtgct
     3481 cgagtgtctg gaggcgatcg cggcgggtga gatctaccag gcgtgtgtgt gcacgcagtt
     3541 caaggggcgc ctcgacggag cgccgatcga cttcttcgtc gaggcggtgg agcgcaccac
     3601 gccggcgcgg gcggcctatg tggccggttc gtggggcgcg gtggcgtcgc tgtcgccgga
     3661 actcttcctg cagcgctctg gggagacagt gacctcgagc ccgatcaagg gcacgctgcc
     3721 gcggcacgcg gatccgttgg cgttgcgggc gtcggtcaag gatgtcgcgg agaacatcat
     3781 gatcgtcgac ctggtccgca acgacttagg tcgcgttgcg ttgacgggga cggtgacagt
     3841 gccggagctg ctggtggtgc gaccggcgcc gggggtgtgg catttggtgt cgactgtgtc
     3901 ggcgcgcgtc ggggtcggcg tgccgatggg atcggtcttg gatgcgacgt tcccgcctgc
     3961 gtcggtgacc ggcacgccga agctgcgggc ccgtcagctg ttgcgcggct gggagccgca
     4021 ccgccgcgga gtatattgcg gcacagtcgg tttggcgtcc ccagtcgcgg gttgtgaact
     4081 gaacgtcgcg atccggacgg tggagttcga cgcgtcaggg aacgcggtcc tcggcgtcgg
     4141 cggaggcatc accgccgact cagatcccga ccgcgaatgg gacgagtgcc tgcacaaggc
     4201 cgccccgttc attgatatag ccgacagcgc tcaacgagta tctcggccaa ccgtttcggc
     4261 tcgctgacca tcaggtcgtg gcacgtgtcc atctcgatga tggtctgtac cccgccgatc
     4321 gccgcgatgc tcctgcgctg cgacttctgt gaaagcgcgc ggtcacgcgt tgtcaggatc
     4381 caagtgcgtg gcacgtcgtc gggcatatcg cgccgggata cgttctcggc caggatgcct
     4441 gggggttcga ggtagagctt gcccaccatg aatcggcgtt gggcgcgggt cattccgttg
     4501 aagaagccga accgagccat cgcgcggggt gtcggggtca atttgccgct cgacgcggcc
     4561 cgtcgggcga tccgcttgaa cagcccagtg agcgtgtccg ctagggcctt gccttcagga
     4621 ggcacgaatg cggtcgcgag gatcatctcg cgcaccctgg acgatccgac cttggtgaga
     4681 acgccgggca cagtcagccc agccatcgag tgcccggcaa gcacgatatc gcctagaccg
     4741 gcctcctcga tgtcggcgac gacggactcc acgaagtccg caatggtgag tgtgtagagg
     4801 tcgccgggct ttccgcggcg gcccggtagg tcgaccgcga gcacggtcag ttctggtgcc
     4861 tgccggtgga gttcgtcgat cgtcggctcc caacaatcgg ctgcgtgccc gccgccgtga
     4921 acgagaacca aggccggcag cgccatggcc ggaccccctt attgccgtga gcgcagaacc
     4981 gcatcgtaga gctcacgtcg cgacggcgcg ccgggattcg cctccaccac ctgtgtgcac
     5041 gcgtccttca cgcggacacc gtcgtcgacc aaggcgttca cttcatcgat cagcgtcgcg
     5101 agatcagcct ttggcgtcgc ccctgccagc acgacggtga tctcgccgag cactttgtcg
     5161 ttcgcccact ccgcgagctc gccgagcgtg ccccgaacga tctcctcgtg cgtcttggtc
     5221 agctcgcggc agaccaccgc cttgcggtcc gcaccgagca cgtcgaccgc gtccttgagg
     5281 gtgtccgcca gccgtcgcgg tgattcgaag aacacgcagg tgcgccgttc ggcggccagc
     5341 gtcttgagcc aggccaggcg cgccgagtgc ttgcgcggcg cgaacccctc gaagcagaac
     5401 cgctccgacg gcagcccagc caccgccagc gccgtcgtca ccgccgaagg gcccggcagg
     5461 caatgcaccg gcgccccagc ctcgatacac gcggtcacca gccggtaacc gggatcgctg
     5521 atcagcggca tcccagcgtc gctgaccagc aggaccgtcg ccccggcctg gatctcgtcg
     5581 accagcgccg gaacccgggt cgcttcgttg gcgtcgtaca ggctgacgac cttgcctgcg
     5641 ggcttgacct cgagcgcctg ggccaggttg cggacccggc gggtgtcttc ggcggcgatc
     5701 acatctgcgc tcgccagggc cttgaccagc cgggcggatg cgtccgacgg ttggcccagc
     5761 ggcgtggcgc cgatgagcag tcggccggtg gtcatgcggg acagcctacg atcgctgtcg
     5821 tgaccgcccc ggccgatgaa ctcgttgcca aggggcgcgc ggtccccgtc atcagtcccg
     5881 cgcccctggt gcccgtcgcc gacttcgggc cggtcgaccg gctgcagggc tgggcgatga
     5941 cggccgtcgt cacggcgctg gccgcggtga cgcgtttcct gaacctcggc tcgccgaccg
     6001 atgcggggac gccgatcttc gacgagaagc actacgcacc gcaggcgtgg cagctgctgc
     6061 gcaaccacgg cgtggaggac aacccgggct acgggctggt ggtgcacccg ccggtgggca
     6121 agcagttgat cgcgatcggc gaggcgctgt tcggctacaa cgggctgggc tggcggttct
     6181 ccggcgcggt gtgcggcgtc atcgtcgtac tgctcgtcgt gcggatcggg cggcgcatca
     6241 gccggtcgac ggtcgtcggc gggatcgcgg gtctgctgtt gatcgccgac ggcgtgagct
     6301 tcgtcgcgtc gcgcaccgcg ctgctcgatg gatttctgga gctgttcgtg gtggcggcgt
     6361 tcggctgttt gatggtcgat cgcgatcagg tgcgcgagcg gatgcacatc gcgctgttag
     6421 agggccgcat cggcgaaacg ccgtgggggc cgcgactggg tgtgcggtgg tggcggttcg
     6481 gcgcaggcgt gtgcctcgga ttggcttgtg caacaaagtg gtccggcctg tacttcatcg
     6541 tgttcttcgg gttcatgacg ctggccttcg acatcgccgc gcggaggcag tatcgggtgc
     6601 cgcggccctg ggtcggggcg ttgcggcgcg acctcggccc gtc
//
LOCUS       DASRJA010000715         8584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2942117,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000715 DASRJA010000000
VERSION     DASRJA010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8584
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2942117"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFC01_32200"
     CDS             complement(<1..277)
                     /locus_tag="VFC01_32200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN84318.1"
                     /translation="MTELKPDLGRVGVWTFGSPGPEQAVEIEKLGYGSLWVGGSPAAD
                     LSFVEPILDQTETLQVATGIVNIWSAEANTVAESYHRIEAAHPGRFLL"
     gene            complement(293..1132)
                     /locus_tag="VFC01_32205"
     CDS             complement(293..1132)
                     /locus_tag="VFC01_32205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN84319.1"
                     /translation="MTDTQKPNLGRFGSFGRGVTPDQAKDIEALGYGAVWVGGSPPAE
                     LDWVEPILEKTTTLQVATGIVNIWTADAGPVAESFHRIDKAYPGRFLLGIGVGHPEAG
                     HEYRKPIDALNDYLDKLDEYGVPKNRRVVAALGPKVLKLSADRTAGAHPYLTTPEHTA
                     EARKLIGADAFIAPEHKVVLTTDADKARTVGRKALEIYLNLTNYLNSWKRLGFSDEDV
                     AKPGSDRLVDAVVAYGTVDAIAARLKEHLDAGADHVPVQVLTSDDKLVPALAELAGPL
                     GLQ"
     gene            complement(1162..2649)
                     /locus_tag="VFC01_32210"
     CDS             complement(1162..2649)
                     /locus_tag="VFC01_32210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN84320.1"
                     /translation="MGDSNKCLPNELRTLFLFEALTDEQLKMLCDNGHIQAFEPGPIV
                     TEGDPATCLYVLLDGELVMSKRSGGVDIETTRTSQRGVYCGAWSAYIPGAESAYAASV
                     RVTKPSRFFVLDADAFARFMQAEFPMAVHLLEGHLVGGLRQRQIIGQREKLLALGQLS
                     AGLTHQLNNPAAATARAVADLRDRVAGMRHKLAMLADGKFTPEALRVLVSIQDEVAEQ
                     VAKSKSVELTALEASDREEQIGDWLEDRGLSGAWEYAPTFVDAGLDTDWLERVSASVD
                     EVDATASLQGAIGWLRYTIETELLMNQIAEASKRISALLAGAKQYSQMDRAEYQSADI
                     HELLHSTVMMFGDKVGKDGAVKMVKDLDKSLPELLCYPGDLNQVWTNIIDNAIQAMDG
                     RGTLTLRTMRENEEMIRVEICDDGPGIPADIIEKIFNPFFTTKPFGEGTGLGLDLARR
                     IVVEKHHGDLRVQSEPGHTKFIVLLPLVAPAPELPTPIELPATAE"
     gene            complement(2659..4356)
                     /locus_tag="VFC01_32215"
     CDS             complement(2659..4356)
                     /locus_tag="VFC01_32215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN84321.1"
                     /translation="MTGPVSAPRKPVILTVDDDPAVSRAVARDLRRHYGERYRIVRAE
                     SGPDALETLNELKLRGDTVAVFVADYRMPQMSGIEFLEEAMDIYPLARRVLLTAYADT
                     HAAIDAINVVDLDHYLLKPWDPPEEKLYPVIDGLLEAWHATGDRAIPHTKVIGHRWNE
                     RSWEVRQFLARNQHSFRSFMADEAKGKQLLDAAGLDGYQLPVVITEQGETLVEPTDAE
                     LAGMLGLSTSPSLEMYELAVIGGGPAGLAAAVYGASEGLKTVLIEETTTGGQAGRSSK
                     IENYLGFPTGVSGAELTTAARRQAERFGAEVITTRKAVRLHAGDSGTARTIEFEDGSD
                     VGAQAVILATGVEYRELQVNGCWNDPDNSACNYVGRGVFYGASVSDSSECKGEDVYIV
                     GGANSAGQAAMYMSRSAKSVTLLVRGPSLETSMSYYLIRQIEKNDNITVRVCTEVVDT
                     LGEDDHLTGLVLFDKQSGEKETVTCDRLCCFIGAAPRTDWLDVVAKDDHGFILSGPDL
                     KDVSGWTLDRPPHHLETSVPGVFVAGDVRAESAKRVAAAVGEGSMAVMLVHRYLAEA"
     gene            4603..4824
                     /gene="infA"
                     /locus_tag="VFC01_32220"
     CDS             4603..4824
                     /gene="infA"
                     /locus_tag="VFC01_32220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZN84322.1"
                     /translation="MAKKDGAIEVEGRVVEPLPNAMFRIELENGHKVLAHISGKMRQH
                     YIRILPEDRVVVELSPYDLSRGRIVYRYK"
     gene            4852..4965
                     /gene="rpmJ"
                     /locus_tag="VFC01_32225"
     CDS             4852..4965
                     /gene="rpmJ"
                     /locus_tag="VFC01_32225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZN84323.1"
                     /translation="MKVNPSVKPICDKCRVIRRHGRVMVICSDPRHKQRQG"
     gene            5147..5521
                     /gene="rpsM"
                     /locus_tag="VFC01_32230"
     CDS             5147..5521
                     /gene="rpsM"
                     /locus_tag="VFC01_32230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZN84324.1"
                     /translation="MARLVGVDLPRDKRMEIALTYIYGVGRTRSQEILEATGIDRNLR
                     TKDLTDDQVTHLRDYIEGNLKVEGDLRREVQADIRRKIEIGCYQGLRHRRGLPVRGQR
                     TKTNARTRKGPKRTIAGKKKAK"
     gene            5525..5938
                     /gene="rpsK"
                     /locus_tag="VFC01_32235"
     CDS             5525..5938
                     /gene="rpsK"
                     /locus_tag="VFC01_32235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008260256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZN84325.1"
                     /translation="MPPAKKAAPKKGAAKTRRREKKNVPHGAAHIKSTFNNTIVSITD
                     PQGNVIAWASSGHVGFKGSRKSTPFAAQLAAENAARKAQEHGVKKVDVFVKGPGSGRE
                     TAIRSLQAAGLEVGAISDVTPQPHNGCRPPKRRRV"
     gene            5954..6559
                     /gene="rpsD"
                     /locus_tag="VFC01_32240"
     CDS             5954..6559
                     /gene="rpsD"
                     /locus_tag="VFC01_32240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZN84326.1"
                     /translation="MARYTGPATRKSRRFGTDLVGGDQSFEKRPYPPGQHGRARVKDS
                     EYRQQLQEKQKARFTYGVMEKQFRRYYEEAVRRPGKTGEELLQILESRLDNVVYRAGL
                     ARTRRMARQLVSHGHFTVNGVKVDIPSYRVSQYDIIDIKDKSLNTLPFEVARETAGDR
                     PIPAWLQVVGERQRILVHQLPERAQIQVPLAEQLIVEFYSK"
     gene            6635..7687
                     /locus_tag="VFC01_32245"
     CDS             6635..7687
                     /locus_tag="VFC01_32245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZN84327.1"
                     /translation="MLISQRPTLAEEVKAENRSQFVIEPLEPGFGYTLGNSLRRTLLS
                     SIPGAAVTSIRIDGVLHEFTTVPGVKEDVTDIILNLKGLVVSSEEDEPVTMYLRKQGP
                     GEVTAGDIVPPAGVTVHNPEMHIATLNEKGKLEVELVVERGRGYVPAVQNKASGAEIG
                     RIPVDSIYSPVLKVTYKVEATRVEQRTDFDKLILDVETKSSITARDALASAGKTLVEL
                     FGLARELNVEAEGIEIGPSPAEADHIASFALPIDDLDLTVRSYNCLKREGVHTVGELV
                     ARTESDLLDIRNFGQKSIDEVKIKLHQLGLSLKDSPATFDPSEVAGYDVATGTWNSDA
                     GYDLDTDQDYAETEQL"
     gene            7714..8328
                     /gene="rplQ"
                     /locus_tag="VFC01_32250"
     CDS             7714..8328
                     /gene="rplQ"
                     /locus_tag="VFC01_32250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZN84328.1"
                     /translation="MPKPTKGPRLGGGSAHQKAILANLATSLFEHGRIKTTETKARAL
                     RPYAEKLITHAKKGALHNRREVLKKIRDKDVVHTLFAEIGPFFADRNGGYTRIIKVEN
                     RKGDNAPMAVIELVREKTVTDEANRARRAAASKKAAPAKAAPAAEVVEEAPAEETVDE
                     VKAEDAAIEEAQTAEAAEEVAEAADEASAEEPTAEAEDKADDAK"
     gene            8261..>8584
                     /locus_tag="VFC01_32255"
     CDS             8261..>8584
                     /locus_tag="VFC01_32255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84329.1"
                     /translation="MPTRPRPRSQRLKPRTRPTTPSSEAVNEPAIDSGGGFVRLRLDI
                     AYDGTEFAGWALQAGQRTVAGVLDDALSTVFRVPVQLRAAGRTDAGVHATGQVAHVDV
                     PTDALP"
BASE COUNT         1554 a         2797 c         2783 g         1450 t
ORIGIN      
        1 cgagcaggaa gcggccgggg tgagcggcct caatgcggtg atacgactcg gcgaccgtgt
       61 tcgcttccgc cgaccagatg ttgacgatgc cagtggcgac ctgcagcgtc tccgtctggt
      121 cgaggatcgg ctcgacgaac gacaagtccg cggccggcga tccgccaacc cacaacgagc
      181 cgtatccgag tttctcgatt tcgacggcct gctccggccc gggcgatccg aaggtccata
      241 cccctacgcg gccaaggtcg ggcttgagtt cagtcatgca agtctccttc gattactgca
      301 ggcccagcgg cccggcaagt tcggcgagcg cgggcaccaa cttgtcatcc gacgtcagca
      361 cctgcaccgg cacgtgatcg gcgcccgcgt ctaggtgctc cttgagccgt gccgcgatgg
      421 cgtccaccgt cccgtaggcg acgacggcat ccaccagacg atcactaccc ggttttgcga
      481 cgtcttcatc actgaacccg agacgtttcc agctgttgag gtaattcgtc aggttgaggt
      541 aaatctcgag cgccttgcgg ccgaccgtgc gggccttgtc cgcgtcggtg gtcaacacca
      601 ccttgtgctc gggcgcgatg aacgcgtcgg ctccgatgag cttgcgcgcc tcggccgtgt
      661 gctcgggcgt cgtcagatac ggatgcgcac cggcagtgcg atccgccgac agcttcaaaa
      721 ccttcgggcc gagggcggcg acgacgcggc ggttcttcgg cacgccgtac tcgtcgagct
      781 tgtccaggta gtcgttgagc gcatcgatgg gcttgcggta ctcatgcccc gcctcggggt
      841 ggccgacgcc gatacccagc aggaagcggc cggggtacgc cttgtcgatg cggtggaacg
      901 actcggcgac ggggccggcg tccgcggtcc agatgttgac gatgccggtg gcgacctgca
      961 gcgtggtggt cttctcgagg atcggctcga cccaatccaa ttcggctggc ggcgaaccgc
     1021 cgacccacac ggcgccgtag cccagcgcct cgatgtcttt cgcttgatcg ggagttactc
     1081 cgcgtccgaa agatccgaat cggccgaggt tgggtttctg ggtatcagtc atggtccggt
     1141 caacccgcgg cgcggcagcg gctattccgc tgtggccgga agttcgatcg gtgtcggtaa
     1201 ttcgggtgcc ggcgccacca atggcagcag gacgatgaac ttcgtgtgcc caggctcgga
     1261 ctgcactcgc aggtcgccgt ggtgcttctc cacgacgatc cgacgggcca gatccagccc
     1321 gaggcccgtg ccctcgccga acggcttggt ggtgaagaac gggttgaaga tcttctcgat
     1381 gatgtccgcg ggaatgccgg gaccgtcgtc gcagatctcg acgcgaatca tctcttcgtt
     1441 ctcgcgcatc gtgcgtagcg tcagcgtgcc gcgaccgtcc atcgcctgga tcgcgttgtc
     1501 gatgatgttc gtccagacct gattgagatc gccggggtag caaagcaatt cgggcagcga
     1561 cttgtcgaga tccttgacca tcttgaccgc gccgtccttg ccgaccttgt cgccgaacat
     1621 catcacggtg ctgtgcagca actcgtggat gtcggcgctt tgatactccg cccggtccat
     1681 ctgggagtac tgcttggccc cggccagcag cgcggagatc cgcttgctgg cctcggcgat
     1741 ctggttcatc aacagctcgg tctcgatggt gtagcgcagc caaccaatgg cgccctgcag
     1801 cgacgccgtc gcgtcgacct cgtcaaccga ggccgagacg cgttcgagcc agtcggtgtc
     1861 gaggccggcg tcgacgaatg tcggggcata ctcccatgcc cccgacaaac cgcgatcctc
     1921 cagccagtcg ccgatctgct cctcgcggtc cgatgcctcc agcgcagtca gctcgaccga
     1981 cttggacttc gcgacctgct cggcgacctc gtcctggatg ctgaccagca cgcgcagggc
     2041 ctcgggggtg aacttgccgt cggccagcat cgccagcttg tggcgcatgc cggccacgcg
     2101 gtcgcgcagg tccgcgaccg cgcgcgccgt cgctgcggcc gggttgttca gctggtgggt
     2161 gagcccagcc gacaactggc cgagcgccag cagcttctcg cgctggccga tgatctggcg
     2221 ctgtctgagg ccgccgacca aatgcccttc gagaaggtgc acggccattg ggaactcagc
     2281 ctgcatgaag cgggcgaacg cgtcggcgtc cagcacgaag aaccgtgatg gcttggtcac
     2341 tcggaccgat gctgcgtagg cactttctgc gccgggtatg tatgccgacc atgcgccgca
     2401 gtacacaccg cgctgcgacg tgcgggtggt ttcgatgtcc acgccgcccg agcgcttgga
     2461 catcaccagt tcgccgtcga gcaggacgta caagcacgtc gcgggatcgc cttcggtcac
     2521 gatggggccg ggctcgaaag cctggatatg gccgttgtca cacagcatct tcagctgctc
     2581 gtcggtcagg gcctcgaaca ggaacagcgt ccgcagttcg ttaggcagac atttgtttga
     2641 atcgcccatc atccgctcct aggcctcggc caaataccgg tgcaccaaca tcaccgccat
     2701 cgaaccttcg ccgacggctg cggccacccg tttggcggat tcggcgcgca catcacctgc
     2761 aacaaacaca ccgggcacac ttgtttccaa atggtgcggc ggacggtcca gtgtccaacc
     2821 gctgacgtcc ttgagatccg gtccggacag gatgaatccg tggtcatcct tcgccactac
     2881 gtcgagccaa tcggtgcgcg gcgcggcgcc gatgaagcag cacagccggt cgcaggtcac
     2941 cgtttccttc tcgccactct gcttgtcgaa caacaccagg ccggtgaggt ggtcgtcctc
     3001 gccgagcgtg tcgaccacct cggtgcagac tcggacggtg atgttgtcgt tcttctcgat
     3061 ctgccggatc aggtagtagg acatcgacgt ctccagcgac gggccccgca cgaggagcgt
     3121 caccgatttc gctgagcggg acatgtacat cgccgcctgc cccgccgagt tcgcgccacc
     3181 cacgatgtag acgtcctcgc ccttgcattc cgatgagtcg gacaccgagg cgccgtagaa
     3241 cacgccgcga cccacgtagt tgcaggcgga gttgtccggg tcgttccagc acccgttcac
     3301 ctgcagctcg cggtactcga cgccggtggc gaggatgacc gcctgggcgc caacgtcgct
     3361 gccgtcctcg aactcgatcg tgcgcgccgt tccgctgtcc ccggcgtgca gcctgaccgc
     3421 cttgcgggtg gtgatgacct cagcgccgaa ccgttcggcc tgacgtcggg ccgcggtagt
     3481 gagctcggcg cccgagacac cggtggggaa gccgaggtag ttctcgattt ttgagctgcg
     3541 gccggcttgt ccaccggttg tcgtctcctc gatcaacacg gttttcaggc cctcggaggc
     3601 gccgtacacc gcggccgcca ggccagcggg tccgccgccg atcaccgcga gctcgtacat
     3661 ctccagcgac gggctggtcg acaggccgag cattccggcg agctcggcgt ccgtcggctc
     3721 gacgagcgtc tcgccctgtt cggtgatgac caccggcagc tgatagccgt ccaggccagc
     3781 ggcgtccaac agctgcttgc ccttggcctc gtcggccatg aacgagcgga acgagtgctg
     3841 gttgcgcgcc aggaactgac gtacctccca cgacctctcg ttccagcggt gtccgatcac
     3901 cttggtgtgc ggaatggccc ggtcaccagt ggcgtgccag gcctcgagga gcccgtcgat
     3961 caccgggtac aacttctcct ccggcgggtc ccacggcttg agcaggtagt ggtccaggtc
     4021 gacgacgttg atcgcgtcga tcgcggcatg cgtgtcggcg taggcggtca gcagcacgcg
     4081 acgggcgagg gggtagatgt ccatcgcctc ttcgaggaac tcgatgccgc tcatctgcgg
     4141 catccggtag tcggcgacga acaccgcaac ggtgtctccg cgcagcttga gttcgttgag
     4201 ggtttccagg gcgtcgggcc cggactcggc ccgaacgatt cggtagcgct cgccatagtg
     4261 gcggcgcagg tcgcgagcga cagcgcggga aaccgcgggg tcgtcgtcga cggtcagtat
     4321 tacgggtttt cggggggcag agactggtcc agtcatcggt tataagtatg ctcccgctgt
     4381 ccaccggata tgtcggcccc ttggccgccc tggcctggca cttcagcgat tttggggcag
     4441 gccgccgagc aggtagtatg gaccaacggt gcggtttccg cgccgactct tgcgtgcccc
     4501 tgttctggaa tttctggtta ccggctcacg ttttgtagtc ggacgaaggc tgcgccaaca
     4561 ggcgccaacg atctacagga cacggaggat ctccgaaaga taatggccaa gaaagacggt
     4621 gccatcgagg tcgagggccg ggtggtcgaa cctctgccca atgcgatgtt ccgcattgag
     4681 ctggagaacg gacacaaggt ccttgcccac atcagcggca agatgcggca gcactacatc
     4741 cgcatcctgc ccgaggaccg cgtcgtggtg gagttgtctc cctacgacct gtcccggggt
     4801 cgcattgttt accggtacaa gtaactagac gagaacagga tcgaccgagc cgtgaaggtg
     4861 aacccgagcg tcaagcccat ttgcgataag tgcagggtga tccgtcggca tgggcgggtc
     4921 atggtgatct gctcagatcc gcgccacaag cagcgccagg gttaaagcgc acttggcgat
     4981 gagccgcttg cgcgaagagc gcaggcgggc cgacaactta atgacgaaat cccagtacca
     5041 ctgagcggat tcgctgctca tccacgtccg gcacggaggc cggaccccat cagacgaggg
     5101 aacggactgg gaccagacct ccgcatagaa gaaggaacac tgcctgatgg cacgcctcgt
     5161 gggcgttgat ctcccgcgcg ataagcgcat ggagatcgcc ctgacctaca tctacggcgt
     5221 cggccgtacc cgctcccagg agatcctgga agccaccggc atcgaccgga acctgcgcac
     5281 caaggacctg accgacgatc aggtcaccca tttgcgcgac tacatcgaag gcaacctcaa
     5341 ggtggagggc gatctgcgcc gcgaggtgca ggccgacatc cgccgcaaga tcgagatcgg
     5401 ctgctaccaa ggcctgcggc atcgtcgcgg cctgccggtg cgcggccagc ggaccaagac
     5461 caatgcgcgc acccgcaagg gcccgaagcg caccatcgcc ggcaagaaga aggccaagta
     5521 accgatgcca ccagccaaga aagccgcccc gaagaagggc gccgccaaga cccgtcgcag
     5581 ggaaaagaag aacgtcccgc acggcgctgc gcacatcaag agcacgttca acaacacgat
     5641 cgtgtcgatc accgatccgc agggcaacgt catcgcctgg gcgtcgtcgg gccacgtcgg
     5701 cttcaagggt tcgcgcaagt cgaccccgtt cgccgcgcag ctggccgccg agaatgccgc
     5761 ccgcaaggcg caggagcacg gcgtgaagaa ggtcgatgtg ttcgtcaagg gcccgggctc
     5821 gggccgtgag accgcgatcc gatcgctgca ggccgccggc ctcgaggtcg gcgcgatttc
     5881 cgatgtcacg ccgcagccgc acaacggctg ccgtccgccg aagcggcgtc gggtctagga
     5941 ggagacagaa cttatggctc gttacaccgg acccgcgacc cgcaagtcgc gccgattcgg
     6001 caccgacctc gtcggtggcg atcagtcgtt tgagaagcgt ccctacccgc ccggccagca
     6061 cggccgtgcc cgcgtcaagg acagcgagta ccgccagcag ctgcaggaga agcagaaggc
     6121 tcgcttcacc tacggcgtga tggagaagca gttccgccgc tactacgaag aggcagtgcg
     6181 tcgccccggc aagaccggcg aggagctgct ccagatcctg gagagccggc tggacaacgt
     6241 ggtttaccgc gccggcctgg cccggacccg gcggatggcc cgtcagctgg tcagccacgg
     6301 tcacttcacc gtcaacggtg tcaaggtcga catcccgagc taccgggtgt cgcagtacga
     6361 catcatcgac atcaaggaca agtcgctcaa cacgctgccg ttcgaggttg cgagggagac
     6421 cgcgggcgac cgcccgatcc cggcgtggct gcaggttgtc ggcgagcgtc agcgcatcct
     6481 ggtgcaccag ttgccggagc gtgcgcagat tcaggtgccg ctcgccgagc agctgatcgt
     6541 cgagttctac tcgaagtaaa gacttctcag ccgtccggat gacggctggg acacgcggca
     6601 tcaaatagtg ggtgccgtga aggaggaaaa aaacatgctg atctctcagc gacccaccct
     6661 ggccgaagag gtcaaggccg agaatcgctc ccagttcgtc atcgagccgc tggagcccgg
     6721 attcggttac acccttggca attcgcttcg gcgcacgctg ctgtcgtcca tcccgggcgc
     6781 ggcggtcacc agcattcgca tcgacggtgt gctgcacgag ttcaccaccg tccccggcgt
     6841 caaggaagac gtcaccgaca tcatcctgaa cctcaagggt ctggtggtgt cctccgagga
     6901 ggacgaaccg gtcaccatgt acctgcgcaa gcagggcccc ggcgaggtca ccgcgggcga
     6961 tatcgtgccg ccggcgggcg tgacggtgca caaccccgag atgcacatcg cgacgctcaa
     7021 cgaaaagggc aagctcgagg tcgagctcgt cgtggagcgc ggccgcggct atgtgcccgc
     7081 cgtgcagaac aaggcatcgg gcgccgagat cggccgcatt ccggtcgatt ccatctactc
     7141 gccggtgctc aaggtcacct acaaggtgga ggccacccgt gtcgagcagc gcaccgactt
     7201 cgacaagctg atcctcgatg tcgagaccaa gagttcgatc accgctcgcg acgcgctggc
     7261 gtcggcgggt aagacgctgg tcgaattgtt cggtctggca cgcgaactca acgtcgaggc
     7321 cgagggcatc gagatcgggc cgtcgccggc cgaggccgac cacatcgcct cgttcgcgct
     7381 gccgatcgat gacctggacc tcacggtgcg gtcgtacaac tgcctcaagc gcgagggtgt
     7441 gcacacggtg ggcgagctgg tcgcccgcac ggagtctgat ctgctggaca tccgcaattt
     7501 cggccagaag tccatcgacg aggtgaagat caagctgcac cagctgggtc tgtccctcaa
     7561 ggacagcccg gccacgtttg atccgtccga ggtcgccggc tacgacgtcg ccaccggcac
     7621 ctggaacagc gacgccggct acgacctgga caccgaccag gactacgccg aaaccgaaca
     7681 gctctaatca cgcaccgtct ataggagtct ccaatgccca agcccaccaa gggccctcgc
     7741 ctcggcggtg ggtccgccca ccagaaggcg attctggcca acctggccac gtcgcttttc
     7801 gagcatggcc ggatcaagac caccgagacc aaggcccggg cgctgcggcc gtacgcggag
     7861 aagctgatca cccacgccaa gaagggtgcg ctgcacaacc ggcgtgaggt gctcaagaag
     7921 atccgcgaca aggatgtggt gcacaccttg ttcgccgaga tcgggccgtt cttcgccgac
     7981 cgcaacggcg gctacacccg catcatcaag gtcgagaacc ggaagggcga caacgccccg
     8041 atggcggtta tcgagctggt gcgggagaag acggtgaccg acgaggccaa ccgggcccgt
     8101 cgcgccgccg cttcgaagaa ggccgcgcct gcgaaggcag ctccggctgc cgaggttgtg
     8161 gaggaagctc cggctgagga gaccgtcgac gaggtcaagg cggaggacgc cgccatcgag
     8221 gaggcgcaga ccgctgaggc agccgaagag gttgccgaag ctgccgacga ggcctcggcc
     8281 gaggagccaa cggctgaagc cgaggacaag gccgacgacg ccaagtagcg aagctgtgaa
     8341 cgaacccgcc atcgattccg gtggcgggtt tgttcgtctt cggctcgata tcgcttacga
     8401 cggaacggaa ttcgcgggct gggccctgca ggccgggcag cgcacggtgg caggcgtact
     8461 ggacgacgcg ctgtcgacgg tgttccgggt acccgtgcag ttgcgtgcgg cgggccggac
     8521 cgacgccggc gtgcacgcaa cgggtcaggt ggcccacgtc gacgttccaa ctgatgcgct
     8581 gccc
//
LOCUS       DASRJA010000716         6471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_382437,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000716 DASRJA010000000
VERSION     DASRJA010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382437"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VFC01_32260"
     CDS             <1..875
                     /locus_tag="VFC01_32260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HZN84330.1"
                     /translation="DLGETNDLFIEHAVEMGCEALSGALEDAGMRPSDVDLIVTTTVT
                     GIAVPSLDARIAGRLGLRPDVRRVPIFGLGCVAGAAGVARLHDYLRGAPDDVAVLVSV
                     EVCSLMFPAAEPTMATLVGSALFGDGAAAVVAVGDRRALLDPAAGPDVLATRSRMYPD
                     SLDIMGWDVGASGFRLVLSPDLPDLIERYFADDVTGFLADHGLTIDDIGSWVSHPGGP
                     RVLEAINAALSLDDAALELSWRSLSEIGNLSSASVLHILRDARAKPPPSETPGLLMAM
                     GPGFCAELVLLRWH"
     gene            878..1381
                     /locus_tag="VFC01_32265"
     CDS             878..1381
                     /locus_tag="VFC01_32265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HZN84331.1"
                     /translation="MVWYALLIAAVAVERVAELVVSQRNLSWSRARGGVEFGARHYPA
                     MVALHTGLLAGCLAESFYRPFLPALGWPMLVVVLAAQGLRWWCIATLGRQWNTRVVVV
                     PDASRVTNGPYRFFSHPNYVAVVVEGIALPLVHTAWVTALVFTVLNAAVLRTRIGVEN
                     TALARLR"
     gene            1378..2394
                     /locus_tag="VFC01_32270"
     CDS             1378..2394
                     /locus_tag="VFC01_32270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN84332.1"
                     /translation="MIDLLVAGGGPAGLATAILGARAGLEVVVIERRHGPVDKACGEG
                     LMPHAVSLLHRLGVEAPGKPFRGITYIDDRRSVTGLFRGGPGRGVRRTALHAGLLEAA
                     TAAGVRIEHGDVGPVSQNATSVYCSGFHARYLAAADGLHSPIRTALGLARPSGGPRRW
                     GIRRHHLMAPWSDCVEVYWSDDAEAYVTPVANDCVGVAVLTSRKGGFDEHLDSFPALQ
                     ERVRGAGHGPDRAAGPLRQKVSHRSAGRVLLVGDAAGYVDALTGEGLGIAFGAAQLLV
                     ESVVADRPQDYDRRWRRMSRRYRLMTAAVLHASAMPALRPRIVPAAAQFPTVFRGVVN
                     LLAD"
     gene            complement(2380..3717)
                     /locus_tag="VFC01_32275"
     CDS             complement(2380..3717)
                     /locus_tag="VFC01_32275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN84333.1"
                     /translation="MSQIGSAALPPGPPLPRWVQTILFMRYWPRFVAACGRRYGKVFT
                     IRAATVPPLVYLSDPAEIKKVFAGDPSIFHAGEANAMLGGLLGDTSVLVIDDDVHRDR
                     RRLMLPPFHREAVAKQTEVMTEIAAANIADWQVGRPFAVAPKMSEITLEVILRTVIGA
                     SDPARLAALRAVMPKLLNVGQWASLAIARPELQRRWPWRSLWDRIQEADQLLYAEIAE
                     RRADPHLDERTDVLAMLVRATDEDGRTLSDRELRDQLMTLLVAGHDTTATALSWALER
                     LTRHPAILDKAVKAADAGDAAGDEYLDAVAKETLRIRPVVFDVGRVLQKPVELAGYQL
                     PAGVMVVPGIGLVHSDSELYPDADRYDPDRMVGATLSPTTWFPFGGGNRRCLGATFAM
                     VELRVVLREILRRVELATTTAPAETQKVKHVIMVPRNGGRIQVRAFRQALIRQ"
     gene            complement(3754..5292)
                     /locus_tag="VFC01_32280"
     CDS             complement(3754..5292)
                     /locus_tag="VFC01_32280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HZN84334.1"
                     /translation="MSVRPSRRSLLKGAVVLAALPLAGFATERRLGPIPADPFSLGVA
                     SGEPAPDGMVIWTRLAPRPLADDGLGGMPARVVDVEWEVAADDRFRRIEQRGITTAGP
                     DAAHSVHVELTGLRPGTDYYYRFRADGYLSPAGRTRTAPEPGSLAPLTMCVASCSNYQ
                     QGFFTAYRRLADEQPDLVVHLGDYQYEYAAGNGNVRDHVGPETVTLANYRQRYAQYKT
                     DPDLQAAHAAAPWLAVFDDHEVANNWADEVPDEPDPAFVARRAAALQAYYENMPLRGS
                     SAPRGIDMQLYRRLNWGALAMFHMLDTRQYRTDQPCGDELRSDCLERLNPAASLTGAD
                     QEQWLLNGFQQSRVQWDVLGQQVFFSQVDLTPGPGRGFNPDAWDGYTANRDRIVAGLA
                     HSPVRNAVVLTGDVHSHWAAQVHERSDDPSSRAVATELVTTSISSGGDGSDSRDEIAA
                     VLPENPHIRYFSGRRGYIRARITAEELRADFRVLPYVSRPGAAVQTGASFVVPDRDAA
                     LYAM"
     gene            complement(5383..>6471)
                     /locus_tag="VFC01_32285"
     CDS             complement(5383..>6471)
                     /locus_tag="VFC01_32285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HZN84335.1"
                     /translation="LDAAVDNASRQRDEALVVARCEYNPSPACPQTHITGVPGSGPET
                     RTATEFLADTQRQLDDAVANRDRLAPGLDNEITTREQALEQARAAATKDVDHGLGARW
                     MAMNDHTVASPGAMLLRLVTISFFALLLLLPLLLRLWRGQTSQDRGAAARAEVERAEL
                     DADTAIAVKRAEVRAAVETLWAEQQLASARMAVEAQMEIDREEHRRRVIEAVEAPVQA
                     ESERVTEPTPALPAAESDNLPALAESDAVESRRESTSLIPEVTKTAARWLRPFVPPII
                     ASAIDTTTRPLRSARQVFEETEEIHFSLKRTHKVSVHSEEHGSVATETAAPQTVVSSV
                     ERQQTMTDREGHPELRGADGLRQLPPAE"
BASE COUNT          973 a         2103 c         2241 g         1154 t
ORIGIN      
        1 gcgacctcgg cgaaaccaac gacctgttca tcgagcacgc cgtggaaatg ggctgcgaag
       61 cgttgtcggg tgcgctcgag gacgcgggta tgcggcccag tgacgtcgat ctgatcgtca
      121 cgacgacggt gaccggtata gctgtgccgt cgctggacgc ccgcatcgca gggcggttgg
      181 ggctgcggcc cgatgtgcgt cgcgtgccga tcttcggcct cggctgtgtg gcgggcgccg
      241 cgggtgtggc gcggctgcac gactacctgc gtggtgcacc cgacgacgtc gctgtgttgg
      301 tgtccgtcga agtgtgctcg ctgatgtttc cggcggccga gccgaccatg gccaccctcg
      361 tgggcagcgc tctgtttggt gacggcgcgg ccgcggtggt ggcggtcggc gatcgtcgcg
      421 ccctgctaga ccccgcggcc gggcccgatg tgcttgcgac gcgcagccgg atgtatccgg
      481 actcgctgga cataatgggt tgggacgtag gcgcttccgg cttccggctg gtgctgtctc
      541 ccgatctgcc cgacctcatc gaacgctatt tcgccgacga cgtcactggc ttcctggccg
      601 accatggcct gacgatcgac gacatcggct cgtgggtgtc ccaccccggc ggcccaaggg
      661 tgctcgaggc gatcaacgcc gcgctcagcc tcgacgacgc cgcactcgaa ttgtcgtggc
      721 gctcattgag cgaaatcggc aacctgtcgt cagcatccgt actgcacatc ctgcgtgacg
      781 cgcgtgccaa gcctccgcca agtgaaacac cgggcctact gatggcgatg ggccccggat
      841 tctgcgcgga actggtgctg ttgcgctggc actgagcatg gtctggtatg cgctgttgat
      901 cgccgcggtg gcggtcgaac gagtcgcgga actagttgtc tcccaacgta atctgtcgtg
      961 gagccgcgct cgcggcgggg tcgagttcgg tgcgcggcac tatccggcga tggtggcgct
     1021 gcacaccggt ctgctggcgg gatgtctcgc ggagagcttc taccgtccgt tcctccctgc
     1081 gcttggctgg ccgatgttgg tggtcgtgct cgctgcgcag gggttgcgtt ggtggtgcat
     1141 tgcgacgctc ggtcgccaat ggaacacccg cgtcgtcgtc gtccccgatg cgtcgcgggt
     1201 gaccaacggc ccgtaccggt tcttctcgca cccgaactat gtggcggtcg ttgtcgaagg
     1261 aatcgcgctg ccgttggtgc acaccgcgtg ggtcacggct ctcgtgttca ctgtgctcaa
     1321 cgctgctgtg ctgcggaccc gtatcggcgt ggagaacacc gcactggcga ggctgcgatg
     1381 atcgacctgc tcgtcgcggg cggcggtccg gccgggctgg ccaccgcgat cctcggtgcg
     1441 cgtgccgggc ttgaagtcgt cgtcattgaa cgacgccatg ggcccgtcga caaggcatgc
     1501 ggcgaaggcc tgatgccgca tgcggtttcg ctgttgcatc gcctcggcgt agaggcgccg
     1561 ggcaagccgt ttcgcggcat cacgtacatc gacgaccgac gcagtgtgac cgggctgttc
     1621 cgcggcggcc ctggacgcgg cgtgcgcagg accgcattac atgccgggct attggaagcg
     1681 gcgacggctg ccggtgtgcg catcgagcat ggcgacgtcg ggccggtgag tcagaacgca
     1741 acatcggtgt actgcagtgg ttttcacgcc cgctacctgg cggccgccga cggattgcac
     1801 tcgccgatcc gcaccgcact cggcctggcg cggccgtccg gtggcccgcg caggtggggc
     1861 atccgccgtc atcatttgat ggcgccatgg agcgactgcg tcgaggtgta ctggtccgac
     1921 gacgcggagg catacgtcac tccggtggcc aacgattgtg tcggcgtcgc ggttcttacc
     1981 tcgcgcaagg gcggtttcga cgagcacctg gactcgtttc ccgcgctgca agaacgggtg
     2041 cgcggtgcgg gccacggccc tgaccgcgcg gcgggtccgt tgcggcagaa ggtgagccac
     2101 cgatcggcgg ggcgggtgtt gctggtcggc gacgccgccg ggtacgtcga cgcgttgacc
     2161 ggagaaggcc tgggcatcgc attcggcgcc gcgcagctat tggtggaatc cgtagtggca
     2221 gaccggccgc aggactatga ccgccgctgg cgccggatgt cgcgccgcta ccggctgatg
     2281 acggcggccg tactgcatgc aagcgccatg cccgcgttgc gtccgcggat tgtgcctgcg
     2341 gcagcacagt ttccgactgt gttccgcggc gtcgtcaacc tactggcgga ttagcgcctg
     2401 ccggaacgct cgaacctgaa tgcgtccgcc gttgcgcggc accatgatta cgtgcttgac
     2461 cttctgggtt tcggcgggtg ccgtcgtcgt ggcgagctcg acgcgccgca gtatctcgcg
     2521 aagcaccacc cgcagttcga ccatcgcgaa ggtcgcaccg aggcaacggc gattgccgcc
     2581 gccgaacggg aaccacgtcg tggggctcag cgtcgcaccg accatccggt ccgggtcgta
     2641 ccggtctgcg tcggggtaca gctcggagtc gctgtgcacc aagccaattc cggggacgac
     2701 catcaccccg gcgggcaact gatagccggc cagttcgact ggcttttgca acacgcgacc
     2761 gacgtcgaac accaccgggc ggattcgcag cgtctccttg gccaccgcgt cgagatactc
     2821 gtcgccggcg gcatctccgg catcggccgc ctttaccgcc ttatcgagga tggccgggtg
     2881 ccgggtcaac cgttccagcg cccacgacag cgcggtcgcg gtggtgtcgt ggcccgcgac
     2941 gagcaacgtc atcagctggt cgcgcagttc acggtcgctt agcgtgcgcc cgtcttcgtc
     3001 ggtggcgcgc accagcatgg ccaacacgtc ggtgcgctca tcgagatgag ggtcggcgcg
     3061 gcgttcggcg atctcggcgt agagcaactg gtcggcttcc tggatgcgat cccacagcga
     3121 ccgccacggc cagcggcgct gaagctctgg gcgcgcgatc gccaacgacg cccactggcc
     3181 gacgttgagc agcttcggca tgacggcccg cagcgctgcg agtcgcgcgg ggtcgctggc
     3241 accgatgact gtccgcagga tcacctcgag cgtgatttcg gacatcttcg gcgcgaccgc
     3301 gaatggtcgg cccacctgcc agtcggcgat gttcgccgcg gcgatctccg tcatcacctc
     3361 ggtttgttta gccactgcct cgcggtggaa cggcggcagc atcaagcggc gccggtcgcg
     3421 atgcacgtcg tcgtcgatca ccagcacaga ggtgtcgccg agcaggccgc ccagcatcgc
     3481 gttcgcctcg ccggcgtgaa agatgctcgg gtcgccggcg aacaccttct tgatctcggc
     3541 gggatcggac agatacacca gcggcggcac cgtcgcggcg cgaatcgtga acaccttgcc
     3601 gtagcgacgc ccgcaggccg ccacgaaccg cggccagtac cgcatgaaca gaatcgtctg
     3661 gacccacctg ggaagcgggg gccccggcgg caacgctgcc gaccctattt gactcatgcc
     3721 taacaggcta caaggggcgc gcggtgttgt gaactacatc gcatacagcg ccgcatcccg
     3781 atccggcacc acgaacgacg ccccggtctg taccgcagcg cccggacgcg agacgtaagg
     3841 cagcactcga aagtcggcgc gcagttcctc cgcggtgatg cgggcacgga tgtagccgcg
     3901 gcgaccgctg aagtaacgga tgtgtggatt ttcgggcagg accgcggcga tctcgtcacg
     3961 tgagtcagaa ccgtcgccac cggagctgat cgaggtggtg accaactcgg tcgcgaccgc
     4021 gcgtgatgag gggtcatcgg acctctcgtg aacctgggcc gcccaatgcg aatggacgtc
     4081 gccggtgagt accaccgcgt tgcgcaccgg cgagtgggcg agccctgcga cgatgcggtc
     4141 gcggttggct gtgtagccat cccaggcgtc gggattgaag ccgcggcccg gaccgggggt
     4201 caaatcaact tgggagaaga acacctgttg tcccaacaca tcccattgca ctcgagattg
     4261 ctgaaaaccg ttgagcagcc actgttcctg gtcggctccg gtcagcgagg cagccgggtt
     4321 caggcgttcg aggcagtccg accggagttc gtcgccgcat ggttggtcgg tgcggtactg
     4381 ccgagtgtcg agcatgtgga acatggccag tgcgccccag ttgagacggc ggtagagctg
     4441 catgtcgata ccgcgcggcg ccgatgaccc gcgcagcggc atgttctcgt agtacgcctg
     4501 cagcgccgcg gcgcgccgag ccacgaatgc ggggtcgggt tcgtccggca cctcgtcggc
     4561 ccagttgttg gccacttcgt ggtcgtcgaa caccgcgagc cagggggctg ctgcgtgggc
     4621 ggcctgcaga tcgggatcgg tcttgtactg ggcgtatcgc tgtcggtagt tggccagcgt
     4681 caccgtttcc ggaccgacgt gatcacgcac gttgccgttg cctgcggcgt actcgtattg
     4741 gtaatcacca aggtgcacaa cgagatcggg ttgctcatca gccagtcggc ggtatgcggt
     4801 gaagaagcct tgctggtaat tggagcacga cgcgacgcac atcgtcagcg gcgctagcga
     4861 gcctggctcg ggcgccgtgc gggtgcggcc cgccggcgag aggtagccat cggcgcgaaa
     4921 ccgatagtag taatcggtgc cgggccgcag cccggtgagt tcaacatgca cgctgtgtgc
     4981 cgcatcgggt ccggctgtcg tgatgccgcg ctgctcgatg cgacggaacc ggtcgtcggc
     5041 ggcgacttcc cactcgacat cgaccacacg tgccggcatg ccgccgaggc cgtcgtcggc
     5101 caaggggcgc ggcgctaatc gcgtccaaat caccatgccg tcaggcgcgg gctcgccgga
     5161 tgccacaccg aggctgaacg ggtcagcggg aatcgggccg agacgacgct cggtggcgaa
     5221 tcccgcgagt ggcagtgcgg cgaggacaac cgcgcccttg agcagcgagc ggcggctcgg
     5281 acgtacagac acggcgcagt gtcatggcgg cgtctgtcca atcggtgacg agaagccata
     5341 cggtgtccgc acccagtgat gcggtttcgc aaacaggcgg aattactccg cgggtggcag
     5401 ctggcgaagg ccgtcggctc cgcgcagttc ggggtgtcct tcccgatccg tcatcgtttg
     5461 ttgacgctcc acagatgaga cgactgtctg aggcgctgcc gtctcagtgg ccaccgaccc
     5521 gtgctcttcc gagtggacgc tcaccttgtg tgtgcgcttg agcgagaagt gaatctcctc
     5581 cgtctcttcg aacacctggc gcgcactgcg cagcggcctg gtcgtggtgt cgatcgcgct
     5641 cgcgatgatc gggggcacga acggacgaag ccaccgtgcg gcggtcttgg tgacctccgg
     5701 aatcaacgag gtgctctcgc gacgcgactc cactgcgtcg ctttcggcca gcgctggcag
     5761 gttgtccgat tccgccgcgg gcagcgctgg cgtcggctcg gtgacccgct ccgactcagc
     5821 ctgcaccggc gcctccaccg cttcgatcac tctgcggcgg tgttcctccc ggtcgatctc
     5881 catctgcgcc tcgacagcca tgcgggcact ggccaattgc tgctcggccc acagcgtttc
     5941 gacggcggcg cgcacctcgg cacgtttgac agcgatcgcg gtgtcggcat cgagctcggc
     6001 gcgttcgacc tcggcgcgcg cagcggcgcc acgatcctga cttgtctgac cacgccacag
     6061 cctgaggagc agcggcaaaa ggagcagcag cgcgaagaac gagatcgtca ccagccgcaa
     6121 cagcatcgcg ccggggctcg cgacggtgtg gtcgttcatc gccatccagc gcgcgccgag
     6181 gccgtggtcc acgtccttcg tcgcggccgc gcgcgcttgc tccagtgcct gctcacgagt
     6241 ggtgatttcg ttgtcgagcc ccggcgccag gcgatcccgg ttggcgacgg cgtcgtccag
     6301 ctgtcgctgg gtgtctgcga gaaattctgt cgccgttcgt gtttcgggcc ccgacccggg
     6361 aaccccggtg atgtgcgtct gcgggcaggc cggcgatggg ttgtactcac agcgggcgac
     6421 gacgagcgct tcgtcgcgct ggcgactggc attgtccacc gcggcatcca g
//
LOCUS       DASRJA010000717         9423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_229094,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000717 DASRJA010000000
VERSION     DASRJA010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9423
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229094"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFC01_32290"
     CDS             <1..352
                     /locus_tag="VFC01_32290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZN84336.1"
                     /translation="RLRAAVRRGTAASDTDEPVIETSSFTVDLAAKKVTKNGTEVHLT
                     PTEWGMLEMLVRNRGKLVGREELLKEVWGPAYAKETHYLRVYLAQLRRKLEDDPSRPV
                     HLLTEAGMGYRFQE"
     gene            826..2829
                     /locus_tag="VFC01_32295"
     CDS             826..2829
                     /locus_tag="VFC01_32295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN84337.1"
                     /translation="MSSPTSVLKRLVVGRPFRSDRLGHTLLPKRIALPIFASDPLSSV
                     AYATQEILLILTLGGLAYLYLTPWIAAAVVLLLAVVTLSYRQVVLAYPSGGGSYEVAS
                     TNLGPRAGLVVAAALLVDYVMTVAVSVAAGVDNVISAIPDLNPYRVAVNIGFIVLLTA
                     MNLRGVRESGRAFAVPTYAFIAGVMLMIAIGLFRTLIGDAPLAESANYGIHAEQAGLN
                     TLAISLLALRAFSSGCTALTGVEAISNGVPAFRKPKSLNAARTMTAMGSLAITMFAGV
                     TAIALISHVRVVEHACDLIGFPGDCNNDAQRTVIAQIAAAVFGNNSVMFFYIQAATAL
                     ILILAANTAYNGFPLLTSILAQDRYLPRQLHTRGDRLAYSNGIILLAVIAGGLIYAFD
                     GSTTRLIQLYILGVFTSFTLCQTGMVAHWNRALRDADTPAQRLRIHRARAINAFGACF
                     TGVVLVVVMITKFTHGAYLVVIAIPVLCVLMQSIHRHYAAVRAELRTADEDEPTLPSR
                     IHAIVLISGWHKATQRALMFAKATRPDTLTALTVNVDDSETRALVREWEELNVDIPLK
                     VIESPYREITRPVVNYIKKQRQSGPRDVVNVYIPEYVVGRWWENLLHNQSSLRLKGRL
                     LFEPSVMVTSVPWQLHSSAHRDLARVEHVAGEIRRGIDRSAIS"
     gene            complement(2916..3200)
                     /locus_tag="VFC01_32300"
     CDS             complement(2916..3200)
                     /locus_tag="VFC01_32300"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN84338.1"
                     /translation="MWSVLPRALIAGAAMSLVLASPAAADPASYLHEVQPVYNNFSAE
                     QLLSEGLRVCKVTRSGMNAPQAVQMVQNDIGASVAAAGDIVAAAVVHLDC"
     gene            3437..3931
                     /locus_tag="VFC01_32305"
     CDS             3437..3931
                     /locus_tag="VFC01_32305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017207224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN84339.1"
                     /translation="MTKGVTADVDPLALERQVCFALAITNRAVLAVYRPLLEPLGLTH
                     PQYLVMLTLWDNAKTNGEPLSVKDIAATLQMDSATLSPMLKRLQAQGLITRSRSETDE
                     RTTHVELTTSGRNLRRQALKVPPAVVERLGVELTELENLHEVLTNINTAALAAGALDS
                     ELDR"
     gene            3928..4341
                     /locus_tag="VFC01_32310"
     CDS             3928..4341
                     /locus_tag="VFC01_32310"
                     /inference="COORDINATES: protein motif:HMM:NF036408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5313 family protein"
                     /protein_id="HZN84340.1"
                     /translation="MSPDAQRRRPDPLRWVWYTYGGTLGPRYRDWVLRDLTGRTRWER
                     QLIRAAVQLAPLVVVVLLVLGSGWIAWVGIGCGIVLALIYSMAYFDPAVEHRLAKHGY
                     PSGTAHRILSERDQAKHPDRMRRYMQTYRDNANCH"
     gene            4371..6128
                     /locus_tag="VFC01_32315"
     CDS             4371..6128
                     /locus_tag="VFC01_32315"
                     /EC_number="2.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC and FAD-binding oxidoreductase
                     domain-containing protein"
                     /protein_id="HZN84341.1"
                     /translation="MATLVSVNVGMPTDVEWQGRVVRTGVFKRPVSGSRMVRRLNIDG
                     DGQGDLAGHGGEQRAVLVYQTESYRYWAEVLGRDDLEPGHFGENFTVDGLPDDEVCIG
                     DRYRIGDAVFEVTQPRVTCYRVGIRLGEPRIAAWLVSHRRPGFYCRVLQEGVVEAGQA
                     IEKIASGPEAVSVTEIDALLYLPSHPQQALQRALRIPALSPGWQESLRSLSDQADRDG
                     ASGNSGLSAAAGTPPPAWRGFRSLRVTDVRAESDTVFSVKLTEPDGAPLPSWLAGQSI
                     ALRIHLNGDDVPLYRNYSLSNRPGDRDYRIAVKQEPGGVASGYLHNHLRVEDRLEVAA
                     ARGGFYLSDGDRPVVLMSAGVGVTPVLAMLHAIAERQPDRQVWWVHASRDGAEHPFAA
                     ETTELLQRLSAVHVHIAYSRPRPSDRLGVDYTIEGRLTTEALRMMALSPEADAYVCGP
                     DSFMRDMTAALTEIGIEKVHTETFGAQGALTPGIAASTSSPPHLPAGPPGAGPDVSFS
                     RSGLTAKWSDRYPSLLDFAEACDVPVRWSCRTGVCHNCETVVLAGTVHYDPDPVEPPA
                     DGNVLICCSRPTSTVVLDL"
     gene            6204..7919
                     /locus_tag="VFC01_32320"
     CDS             6204..7919
                     /locus_tag="VFC01_32320"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HZN84342.1"
                     /translation="MPGKACARRVGGLAVALGVAAAALTGWNSGIAWADESGDAAGAT
                     ASSTPSDTDSATGNSSPAQEAAKSGDTETTTTNDDDVRTAPPGVVVGTGGLIEEPSTD
                     PTPEPSETKPTTKKGSDPAEAKPTKKKTPPPESRVTDAKDDATPTGAAARDVATDTVT
                     QRSDTTEPPGTASLRIASTQSLVATQTPQLTTEPTAKPMPPNLVTVVSRIVTGVVNAI
                     LSPFAAINTPGAPADTPSIWALLAFARREFEKAFTSPSLTDKPAEPNTTSQTEPEQRT
                     LILTAFPAETDAILSRTTLDPNPTVVVDGHHYYLGTLGGKKVIVAMTGIGMVNATRTT
                     ETALDHFTPESGISIDAVVFSGVAGGSGRTEIGDVAVPARWTSDDGETWHAVDAGMLA
                     TANTLDVDLLSSDTLGDPACYCGLLAGPRIDLGREPDLHVGGDGSSDDNNNGTAFPAI
                     PLGGDVFGPQPCRAPDYSLLSTGNFFQAVVPFLAFGLLSNITGFLTDTPPAVDAVDQE
                     TAAAQQVADAHGIPFLGIRGMSDGPGDPLNLPGYPFSFLVYKQIAADNAAIVTEAFLQ
                     NWDDT"
     gene            complement(7931..8401)
                     /locus_tag="VFC01_32325"
     CDS             complement(7931..8401)
                     /locus_tag="VFC01_32325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN84343.1"
                     /translation="MAEPAAIDSRLTVKRDTTATRQQVWDVLANGWNYSQWVVGNTRM
                     RAVDPRWPAPGSTIHHTIGVWPVVLNDETEVESSTPLEELVLLAKGRPFGGARIVLRL
                     FDTQTGCRIEMAEVPVGGPLNLLPRRLALAAAYPRNRECLARLAALAERREQPE"
     gene            complement(8447..9310)
                     /locus_tag="VFC01_32330"
     CDS             complement(8447..9310)
                     /locus_tag="VFC01_32330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN84344.1"
                     /translation="MTSEPPPIEGVRRSFVEARGVRFHVTESGPADGRPVLALHGWPQ
                     HHWAYNDLLADPPPGLRIIAPDLPGYGWSGPAPHKWAKEDVASDVLALMDALGLDRVL
                     LVAHDWGGYIGYLMVLGAPERFDGYLALNMAYPWVTARDVLPHIWRWLKYQPLIAFLG
                     VPVQRYTNFLHLAFKVGSALDPETARVYVDRFRDPVVARSARDSYRTFLLRELPRGAR
                     HPETRRATVPIRALFGVHDTAIHVSLVSPEHANADDYTLETVDATHFIIDERPDLVRA
                     RLVALADETKR"
BASE COUNT         1677 a         3122 c         2973 g         1651 t
ORIGIN      
        1 tcggctgcgg gccgcggtac gccggggcac tgccgcgtcc gacaccgacg agccggtgat
       61 cgaaacctcg tcgttcaccg tcgacctcgc cgcgaagaag gtgaccaaga acggcacaga
      121 ggtccacctg acccccaccg agtggggcat gctcgagatg ctggtgcgca accgcgggaa
      181 gctcgttggc cgcgaggagt tgctcaagga agtgtggggc ccggcctacg ccaaggaaac
      241 ccactatctg cgagtgtatt tggcgcagtt gcggcgcaag ctcgaggacg acccgtcacg
      301 cccggtccac ctgctgaccg aagctgggat gggttatcga ttccaggagt gatcccggtc
      361 cgtctctgca cggttgtaca tcgcagtcca gagcgagtag cacgatagtg cctcgacaac
      421 cacacgaccg atgaccgtgt gtcggtacac cgtgtcggcg ggcgcgtcgg tgtcccagct
      481 tccatggtcg ttcctggtca ccagtccacg agccccgagg agtctcaacg ccgccgccaa
      541 tccgcgccgg ccagcgtggg cgtgggtgcc cagatctgca cgggtcattg gcccgcgggt
      601 gagcgcgtcg agtacttcaa gcacgtgcgc gtggcttact agctcaagca tgtccggcac
      661 agagtccggt tcaccgatgg cgggcattcg ccgcacggta gatatcaggc ctgcagcggt
      721 aaatatcgcc ttacggcatc cgtacgtcgg cttgctcgac ttttacgaaa tctatacgcc
      781 tccggatggg tgctctccgg cgccgcgaca ttaggctcgc tgatcgtgtc gtcaccgaca
      841 tccgtgctga aacgcctggt cgtcggccga ccgttccgca gtgaccgact cggccatacc
      901 ctgctgccga aacgcatcgc actgccgatc ttcgcgagcg acccgctgtc ctccgtcgcc
      961 tacgcgaccc aggagatcct gctgatcctg accctcggcg ggcttgccta cctctacctc
     1021 acgccgtgga tcgcggcggc ggtggtgctg ctcctcgccg tcgtgacgtt gtcgtatcgc
     1081 caagtggtgc tggcctaccc cagcggtggc ggttcctacg aagtggcgtc gacgaacctg
     1141 ggtcctcgtg cgggactggt ggtggcggcc gcactgctcg tcgactacgt gatgacggtg
     1201 gcggtctcgg tggccgcggg cgtggacaac gtcatctcgg cgatccccga tttgaacccg
     1261 tatcgcgtcg cggtcaacat cggtttcatc gtcttgctga ccgcgatgaa tctgcgggga
     1321 gtgcgcgagt ccggtcgagc gttcgccgtg ccgacctacg ccttcatcgc cggggtgatg
     1381 ctgatgatcg caatcggctt gttccgaacg cttattggtg atgcgccgct agccgagagt
     1441 gcgaattacg gaatccacgc cgaacaggcg ggcctgaaca ccctggcgat ctccttgctg
     1501 gcgctgcgcg ccttctcgtc tggctgcacg gcgctcaccg gggtagaagc catctccaac
     1561 ggagtgccag cgttccggaa gcccaagtct ctcaacgccg cccggacgat gaccgccatg
     1621 ggcagtctcg cgatcacgat gttcgccggc gtcaccgcga ttgcgttgat atcgcacgta
     1681 cgggtggtcg aacacgcctg tgatctcatc ggatttcccg gcgactgcaa taacgacgca
     1741 cagcgcacgg tgatcgccca aatagccgcg gcggtgttcg gcaacaacag tgtgatgttc
     1801 ttttacattc aggccgcgac ggcgctgatc ctgatcctgg cggcgaacac cgcttacaat
     1861 ggctttcccc tcctcacctc gatcctcgcc caggaccgtt acctgccccg ccagctgcac
     1921 acccgcggtg accgactggc gtactccaac ggcatcatcc tgcttgcggt catcgccggc
     1981 gggttgatct acgcgttcga cggctcgacg acgcggttga tccagctcta catcctcggc
     2041 gttttcacgt cattcacgct ctgccagacg ggcatggtgg cgcattggaa tcgggcattg
     2101 cgcgacgccg ataccccagc acagagactc cgtatccacc gcgcgcgggc catcaacgca
     2161 ttcggcgcct gtttcaccgg tgtggtcttg gtggtggtca tgatcaccaa gttcacgcac
     2221 ggcgcctatc tcgtggtcat cgcgattccg gtgctttgcg ttctgatgca gagcattcat
     2281 cggcactatg cggcggtgcg tgccgagcta cgcaccgcag acgaagacga gccgacgttg
     2341 ccgagcagga tccacgccat cgtactgatc tcgggctggc acaaagcgac tcagcgtgcc
     2401 ctcatgttcg cgaaggcgac gaggcccgac actctcaccg cgctgaccgt caatgtcgac
     2461 gactctgaga ccagggccct ggtgcgggaa tgggaggaac tcaacgtcga catcccgctc
     2521 aaggtcatcg agtcgcccta ccgcgagatc acccgtcccg tcgtcaacta catcaagaaa
     2581 cagaggcaga gcggtccgcg cgacgtggtc aatgtgtaca tacccgaata cgtcgtcggc
     2641 cgctggtggg agaacctgct gcacaaccag agttcgttgc gcctcaaggg tcgattgttg
     2701 ttcgagccga gcgtgatggt gaccagcgtg ccctggcagc ttcactcgtc ggcgcaccgc
     2761 gaccttgccc gtgtcgaaca cgtcgcgggc gaaatccgca gaggcattga ccgttccgcg
     2821 atcagctagt cgcgttcaag gcctactccg gctggcaaga cacaaagtcg caatacacgc
     2881 gatttctggt ctcactcgcg caggagctgt ccacttcagc agtcaaggtg aaccacagcg
     2941 gctgcgacga tatcgccggc cgcggccacc gacgccccaa tgtcgttctg caccatctgg
     3001 acggcttgcg gggcgttcat gccgctgcgg gtgaccttgc agaccctgag accctcggac
     3061 agcaactgct ctgcgctgaa gttgttgtag acgggttgca cttcgtgaag gtagcttgcc
     3121 gggtcagccg ctgcgggcga ggcaagcacg agcgacatcg ccgcgccggc gatcagggct
     3181 cgaggcagga cggaccacat gtcagaggtg aactccagta cgtttgctgt tccgactgcc
     3241 gacgttaccg gcattcaccg ctgatcgggc tgcttttggt ccgttccacg gcgtgatggt
     3301 ggcactcacc agaacctcct tcaacccccc gattcctctg tcggggtcgt atgcgggtcc
     3361 aacggctggg cccgaggaaa tgtgatctat aggacactaa tggatagtgc cctaactatg
     3421 tgggtacgat ggagccatga ccaaaggagt caccgcggac gtcgatccgc tcgcattgga
     3481 gcgccaggtg tgtttcgcgc tcgccatcac caaccgcgcg gtgctcgcgg tgtaccggcc
     3541 gttgctcgag ccgctcggac tgacccatcc gcaatatctc gtgatgctga cgctgtggga
     3601 caacgcaaag accaacggcg agccactgtc cgtcaaggac atcgccgcga cattgcagat
     3661 ggattcggcc actctgtcgc cgatgctcaa acggctacag gcccaggggc tgatcacccg
     3721 gtcacgcagt gagaccgatg aacgcaccac ccacgtggag ctgacgacca gcggccgcaa
     3781 tctgcgccgc caggcgctca aggtcccccc ggcggtggtc gagcgcctcg gcgtcgagct
     3841 gacggagctc gagaatctgc acgaggtact cacgaatatc aacaccgccg cattggcggc
     3901 cggtgcgctc gactcggagc tggaccgatg agccccgatg ctcagcggcg ccgccctgat
     3961 ccgctgcgct gggtctggta cacctacggc gggacgctcg ggccgcgcta ccgcgactgg
     4021 gtgttgcgtg acctcaccgg gcgcacccgg tgggagcgcc aactcatccg cgccgcggtg
     4081 caactggcgc cgctggtcgt ggtggtgttg ctcgtcctcg gatccggctg gatcgcctgg
     4141 gttggcatcg ggtgcgggat cgttctggcg ctgatctact cgatggccta cttcgatccg
     4201 gcggtggagc accgtctggc caagcacggc tatccgtccg gcaccgcaca ccggattctc
     4261 agcgagagag accaggctaa gcatccggat cggatgcgcc gctacatgca gacgtatcgt
     4321 gacaacgcca actgccactg aggtcgtcag cacgccctgg gggtgaagac atggccactc
     4381 tcgtctcggt gaatgtcggg atgccgacgg atgtggagtg gcaaggccgc gtggtgcgga
     4441 ccggggtctt caagcgacct gtgagcgggt cgcggatggt gcgccgcctc aacatcgacg
     4501 gtgacgggca gggtgatctc gcgggtcacg gtggcgagca gcgcgccgtt ctggtctatc
     4561 agacagagtc gtatcgctat tgggcagaag tgttgggccg ggacgatctt gagccaggcc
     4621 atttcggcga gaatttcacg gttgacggcc tgcccgacga cgaggtgtgt atcggcgacc
     4681 gctatcgcat cggcgacgcc gtcttcgagg tgacacaacc gcgggtcacc tgctatcgcg
     4741 tcggaatacg actcggcgag ccgaggatag ccgcatggct ggtgtcgcat cgcaggccgg
     4801 gcttctactg ccgggtgctg caagagggtg tggtggaagc cggccaggcc atcgagaaga
     4861 tcgcgtcggg cccagaagcg gtgtcggtca cggagattga cgcattgctg tacctgccga
     4921 gccatccgca acaggcgttg cagcgcgcgt tgcgcattcc cgcgttgagt cccggctggc
     4981 aggagtcgtt gcggagtctc tcggatcaag ccgaccgcga cggtgccagc ggcaacagcg
     5041 ggctcagcgc ggccgccggg acaccgccgc cggcgtggcg tggcttccga tcgttgaggg
     5101 tgaccgatgt ccgggcggag tccgacacag tgttttcggt gaaacttacc gagccggatg
     5161 gtgccccgtt gccgagttgg cttgcgggac aatccattgc gctgcgcatc cacttgaacg
     5221 gcgacgacgt gccgctctat cgcaattact cgctgtcgaa ccggcccggt gatcgggatt
     5281 accggatcgc ggtcaagcaa gagcccggcg gtgtcgccag cggctacctg cacaaccatc
     5341 tgcgggtcga agatcgtctc gaggtcgccg ccgctcgcgg cgggttctac ctgagcgacg
     5401 gagaccggcc ggtggtcctg atgtcggccg gcgttggggt caccccggtg ttggccatgc
     5461 tgcacgcaat cgccgaacga caaccagacc gccaagtgtg gtgggtgcat gcgtcgcgtg
     5521 atggcgctga gcatcccttc gcagccgaaa ccaccgagct gctccagcga ttgtctgccg
     5581 tgcatgtgca tattgcctac agccggccgc gcccttcgga tcgactcggg gtcgactaca
     5641 cgatcgaggg acggctgacc acagaggccc tgagaatgat ggcactgtca ccagaggcgg
     5701 atgcctacgt gtgcggcccg gattccttta tgcgcgacat gactgccgca ctaaccgaga
     5761 tcggaatcga gaaggtccac accgaaacgt tcggcgccca aggcgctttg acgcccggca
     5821 tcgccgcctc gacatcatcg ccgccacacc tacctgccgg cccgcccggt gcaggcccgg
     5881 acgtgtcgtt ctcgcgaagc ggcctcaccg ccaagtggag tgaccggtac cccagcctgc
     5941 tggacttcgc cgaggcgtgc gacgtgcccg tccgctggtc atgccgcacc ggggtgtgcc
     6001 acaactgcga aaccgtggtc cttgcgggca cggtgcacta cgacccggat ccggtcgagc
     6061 ctccggcaga cggcaacgta ttgatctgct gttcgcggcc cacctcgacc gtggtcttgg
     6121 acctgtgacc gacgaacact ggcaaactgc ggagttcctc gcattccggc gcgccgattg
     6181 acctcacgcg gtatacatgc cgcatgcctg gcaaggcatg cgcaagacgg gtcggcggcc
     6241 tggccgtcgc cttgggcgtc gccgctgccg ccctgaccgg ctggaacagc ggcatcgcct
     6301 gggccgacga gtccggcgat gccgcagggg cgactgcctc cagcactccg tccgacacgg
     6361 actcagcgac aggcaactcc agcccggcgc aagaagccgc aaagtcgggc gacaccgaga
     6421 cgacgaccac caacgatgac gacgtgcgca cggcgcctcc gggcgtcgtt gtcggcacgg
     6481 gcgggctcat cgaggagcca tcgacagacc cgacgccgga gccgagcgag acgaagccca
     6541 caaccaagaa ggggtccgac ccggctgagg ccaagcccac aaagaagaaa acgccacccc
     6601 cggaatcccg ggtcaccgac gccaaggacg acgcgacacc gaccggagcc gcggcgcgcg
     6661 acgtggccac cgacaccgtt actcaaagat ccgatacgac ggaaccgccg ggaaccgcgt
     6721 cactgcggat cgcatcgaca cagtcactag ttgccacgca aactccacaa ctcacgacag
     6781 agccgacggc aaagcctatg ccgcccaacc tcgttactgt cgtgagcagg attgtgacgg
     6841 gagtcgtcaa cgcgatcctg agcccgttcg ccgcgatcaa caccccggga gcgcccgccg
     6901 acaccccatc gatatgggcg ttgctggcgt tcgcgcgacg cgagttcgag aaggcattca
     6961 cgtcaccatc gctgaccgac aaaccggccg agcccaatac aacgagtcag acggaacccg
     7021 aacagcgcac gctgatcctg accgcgtttc cggccgaaac ggacgcgatc ctgtcccgca
     7081 ccacactcga cccgaacccc accgtcgtcg tcgacgggca ccattactac ctcggcacgc
     7141 tgggcggcaa gaaggtcatc gtcgcgatga ccggcatcgg catggtgaac gccacccgca
     7201 ccaccgaaac cgcgctcgac cacttcacgc ccgagtccgg aatctccatc gacgcagtgg
     7261 ttttctccgg cgtcgccggc ggctccggac gcaccgagat cggcgatgtg gctgtgccgg
     7321 cccggtggac ctccgatgac ggagaaacat ggcacgcggt cgatgccggc atgctcgcca
     7381 ccgccaacac actcgacgtc gacctgctca gcagcgacac cctcggcgat cccgcctgct
     7441 attgcggtct tctcgcaggc ccgcggatcg acctggggcg cgagcccgat ttacatgttg
     7501 gcggcgacgg ttccagcgat gacaacaaca acggcacggc gttccccgcc atccccctcg
     7561 gcggcgacgt gttcggcccc caaccctgcc gcgcaccgga ttactcactt ctgtccactg
     7621 gcaacttctt ccaggcagtc gtgcccttcc tcgccttcgg gctgctcagc aacatcacgg
     7681 ggttcttgac ggatacgcct cctgctgtgg atgcggtcga ccaggagacc gcggcggcgc
     7741 aacaggtcgc cgacgcacac ggcatcccgt tccttggcat tcgcggcatg tccgacggac
     7801 ccggcgatcc gctgaacctg ccgggctatc ccttctcgtt cttggtctac aagcagatcg
     7861 cagcggacaa cgccgccatc gtcaccgaag ccttcctaca gaactgggac gacacctgat
     7921 ctgtgcacac tcactccggc tgttcccggc gttcggcgag cgcggccaac cgtgccaggc
     7981 attcacggtt ccttggatat gccgccgcca gggccagtcg gcgcggcagc aggttaagcg
     8041 ggccacctac cggcacctcg gccatctcga tgcggcagcc ggtttgagta tcgaacagtc
     8101 tcaagacaat tcgggcacca ccgaaaggcc tgcccttggc aagcaatacc agttcctcga
     8161 gcggcgtgga ggattcgacc tcggtctcgt cgttgagcac caccggccac acgccaatcg
     8221 tgtggtggat cgtgctgcct ggcgcgggcc atctgggatc caccgctcgc atgcgagtgt
     8281 ttccgaccac ccactgtgaa tagttccagc cgttggccag cacgtcccac acctgctgcc
     8341 gggtggcggt ggtgtcgcgc ttcacggtga gtcggctgtc gatcgccgct ggttctgcca
     8401 tacccgcggg ctacccgacg gcgacggcgc caaaccgccc cgatagtcag cgcttggttt
     8461 cgtcggcgag tgcgaccagc ctcgcccgca ccaggtcggg ccgctcatcg atgatgaaat
     8521 gggtcgcatc gaccgtttcc agcgtgtagt cgtcggcgtt tgcgtgttcg ggcgacacca
     8581 gcgacacgtg gatggcggtg tcatggacac cgaacaacgc gcgaatcgga acggtggcac
     8641 gccgtgtctc gggatgcctt gcaccccttg gtaactcgcg tagcaagaag gtccggtagc
     8701 tatctcttgc cgagcgcgcg accaccggat cgcggaaccg gtccacgtaa acgcgtgccg
     8761 tttcgggatc gagcgctgag ccgaccttga aggcaagatg gaggaagttg gtgtagcgct
     8821 gcaccggcac gccgaggaat gcgatcagcg gctgatactt cagccagcgc cagatgtgcg
     8881 gcagcacgtc ccgcgcggtc acccacgggt atgccatgtt cagcgcgaga tagccgtcga
     8941 aacgctccgg cgcgcccagg accatcaggt atccgatgta accgccccag tcgtgtgcga
     9001 cgagcagcac gcggtcgagt ccgagcgcat ccatcagagc gagaacgtcg ctggcgacgt
     9061 cctccttcgc ccacttgtgc ggggccgggc cggaccaccc gtagccgggc aggtcaggcg
     9121 cgatgatccg caggcccggc ggtgggtcgg ccaacagatc gttgtacgcc cagtggtgct
     9181 gcggccagcc atggagtgcg aggacagggc gaccgtcggc cgggcccgat tccgtgacgt
     9241 gaaagcgcac tccccgcgct tcgacgaacg agcgtcgaac gccttcgatg ggcggcggtt
     9301 cggaggtcat gaatcggact ctacgtaacc ggtgccgcgc taccgtcggc gtatgggtgg
     9361 atatcgcgac ctgttcaatg ccagcctcgc cgatccgacg gcgttttggg ccgacgccgc
     9421 acg
//
LOCUS       DASRJA010000718        25235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3202586,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000718 DASRJA010000000
VERSION     DASRJA010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 25235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25235
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3202586"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VFC01_32335"
     CDS             <1..548
                     /locus_tag="VFC01_32335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN84345.1"
                     /translation="QITERKAIGIDDYMRTNVPHIYAIGDVTGKLQLAHVAEAQGVVA
                     AETIGGADTLPLGDYRMMPRATFCQPQVASFGLTEEQARDEGYDVKVAKFPFTANAKA
                     HGMGDPSGFVKLIADGKYGELIGGHLIGHDVSELLPELTLAQKWDLTANELARNVHTH
                     PTMSEALQECFHGLVGHMINF"
     gene            545..814
                     /locus_tag="VFC01_32340"
     CDS             545..814
                     /locus_tag="VFC01_32340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84346.1"
                     /translation="MRAIWVAGIGGLVVGHILWLAGISVAIATRTINSWVLIVAGVSF
                     VAGAIAAYFGWRSYQRKSEIWAAFLWALPISPVLLSLTVLGVTYL"
     gene            877..2565
                     /locus_tag="VFC01_32345"
     CDS             877..2565
                     /locus_tag="VFC01_32345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN84347.1"
                     /translation="MKSVAVLGGGVGGLSAAHELAERGLKVTVYEMRDAFGGKARSMP
                     VPGSGTGGRKDLPGEHGFRFFPSFYKHIIDTMGRIPYGQGTVAGNLIETTKILLAQGG
                     DRNELPAPTVPPSSVNDVVAALKFMREFVFGLGIPISEFLDYTKGLLTLLVSCDGRRY
                     GEWEEMSWWDFMDAGHRTEAFNKFLGDGITRCLVAARGREMSARTGGMISLRLMLNSG
                     APVDRVLKGPTSEVWIDPWVEHLRGLGVELRGGHTVGGIHCDGREITGVTVTGPHGDE
                     LITADYYIAAMPKEKLELLISPQMCAADPHLAALGRLQTRWMNGAMFYLDTDVPLEHG
                     HIIFIDSEWSLTAISQAQFWKRVVDLKQRGDGRVEGILSVDVSEWRTPGSTGKVASAC
                     TKDEICDEVLKQMQDHIKDRKLDKANVVTCFLDPAISFPNPSQAANAEPLLINTPGSW
                     ADRPEAWTAIPNFFIAADFVRTDTDLATMEAANEAARLAVNAILKATGSKEGCELWKF
                     PEPCLLAPFRLMDRLLWRLGRKPPKLPVRMTSTGEVEPTGPVARTVLAVTRRLR"
     gene            complement(2624..5401)
                     /locus_tag="VFC01_32350"
     CDS             complement(2624..5401)
                     /locus_tag="VFC01_32350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN84348.1"
                     /translation="MPVSGPSLDDAAVGDLLTAAKTRPSALLIQGEAGIGKTTFWQFA
                     VDRARERGFRVLTARASQAETVHAYATVGDLVADVEPTLMARIPDLQHRALDRVLMRA
                     SGEGPEANQQMVAAAFMSVIEALSANAPPLIAVDDVHWLDSSSRAVMAFAVRRLKNRV
                     GVLFTEREEHDTVNALSWLQLATGDEIGKVRVSPLGLHELHALIKNRIGRSFPHPTMV
                     RIAEVSGGNPLYALELARAIDAEGGGEHQVFPRSLANLVRMRIARLDGRALDVLVAAA
                     CLPQPTVDMLVGVTGTTVEEVVELLEEPESAGIIALDGNRVSFTHPVLARGVYANAAP
                     ARRRYWHRAWSEALSEPESKARHLALAATSSDQETLAALDAAAAVVRGRGAPAAAAEL
                     LDLAIGLGGDTPMRRFAAAEHHLRSGDAARAQEVLKPGIDQMEPGPFRALMMFGYAVI
                     LMHTEGYSQAADVLYEAIDDATGDPTVLVEVLMMLAFAQINEGIYETAPQHARRALAE
                     AEAAAQPVLISQALASWAYVSFHCGSGYDETAMTRALELEDHAGDVPIPFRASAVDAL
                     LMAWTGRLEESATALDALTTQLVRRGGEIEMLFVAYNSVLVNVWLGRYVEARHFAEDT
                     FRRVEEIGGSAIRGLGLAMRGWVAVHAGHEQEARADLTAALESAARSESSSWWVDLAL
                     TGLARLEVSAGRHAEALNVLAPLLAVADTVPNTEISKMEYLPDAIEAMVISGRFEEAE
                     VLIDRLERDGRELGRAWMVLVAARCRGMWLAASGDVEAAVRVVAQAADAHRGLRMPLE
                     HARTQLLLGQLYRRLRQKQAAAAALDQALRTFEQLDNPLWAARVHAERARVNVRPGHG
                     VDLTPSEQRVAELAAEGLSNREIAAAVYVSAKTVESVLTRVYRKLGVRSRAMLGRRML
                     ELAPRDETLDN"
     gene            5552..5962
                     /locus_tag="VFC01_32355"
     CDS             5552..5962
                     /locus_tag="VFC01_32355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84349.1"
                     /translation="MTAARRWLLLANGVFLVVVGGVQVVFELLAYYKGVGPYGYIFEE
                     SPYAIGGVEAHGLAFIIGVLLLAVAVRDLRRFWHILSAVVHTLLGAANLVFWNSFIVF
                     GMVPMGAAATVVHFVLVAAHVAAFVVSRGTARER"
     gene            5940..6623
                     /locus_tag="VFC01_32360"
     CDS             5940..6623
                     /locus_tag="VFC01_32360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84350.1"
                     /translation="MGLLESVERIYHRFAAYPCPANLWVCEQCGPEWSTEDIRALPLR
                     SVSSLPQLVAVHVMSLDVDGLRHFFPRLMELMLQKPSPVFDFRLADLKDRLRGWRPEE
                     VGCVRDLADAVWAELLVGYPPALGYFSDCPSALDLLDWCELPLVKYLGLLLTVDGLPA
                     ARHVADLIDAVFTMREPFESASKSTVSEWITDPSVGERLQAAFFTAPIEEAAWQLSAA
                     HELWTVCAR"
     gene            6684..6992
                     /locus_tag="VFC01_32365"
     CDS             6684..6992
                     /locus_tag="VFC01_32365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84351.1"
                     /translation="MAASKYAVISFAGAVLAGCALALAANAQADDNCDPFLLSMTPQP
                     VLACQAPDVAPPPDQPAVAPVNDVDVPLPAEVPPPAEPGPFGDVQAPAPLLLPDGSVR
                     "
     gene            complement(7053..7349)
                     /locus_tag="VFC01_32370"
     CDS             complement(7053..7349)
                     /locus_tag="VFC01_32370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HZN84352.1"
                     /translation="MATDYDAPRVTEPDADESLEELTSRRKNDAATAVIDVDDGDAVD
                     SFDLPDADLTGEELTVRVVPKQADEFVCSSCFLVQHHARLALRKDGQLICTDCV"
     gene            complement(7420..7962)
                     /locus_tag="VFC01_32375"
     CDS             complement(7420..7962)
                     /locus_tag="VFC01_32375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN84353.1"
                     /translation="MSAPARIPPGGFKELGPINWAIAKLGARGIRAPKFHLFNVLGQH
                     KLLFLAWLPFSGYLLYAGKLSRKDAELVILRVGHLRDCEYELQQHRRLAKSRGVDAEL
                     QARIFEGPDADGLNDRQRVLITATDEFVITRSMSPETWAALSGIYNRAQLIEFCTLAG
                     QYDALAATMATLKIPLDFPD"
     gene            complement(7959..9374)
                     /gene="ramB"
                     /locus_tag="VFC01_32380"
     CDS             complement(7959..9374)
                     /gene="ramB"
                     /locus_tag="VFC01_32380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate metabolism transcriptional regulator
                     RamB"
                     /protein_id="HZN84354.1"
                     /translation="MAKTFVGSRVRQLRSERGFSQAALAQMLEISPSYLNQIEHDVRP
                     LTVAVLLRITEVFGVDATFFASQDDTRLVAELREVTMDRDLDIDVDLPEIADMVGSHP
                     ALARAMVNLHRRYRLTTTQLAAATEDRNSDGSGTGSITMPHEEVRDYFYERQNYLHEL
                     DTAAEDLTIRMRMHRAELARELSDRLTRVHGVHIIRRIDLGDTVLHRFDPTTKTLEIS
                     GHLSSGQTVFKMAAELAYLEFGDLIDKLVDEGKFTSEESTTLARLGLANYFAAATVLP
                     YRQFHDVAENFRYDIERLSAFYSVSYETIAHRLSTLQRPSMRGVPLSFIRVDRAGNMS
                     KRQSATGFHFSSSGGTCPLWNVYETFANPGKILVQVAQMPDGRNYMWVARTVERRASR
                     YGQPGKTFAIGLGCELRHAHRLVYSEGLDLSGDNATPIGAGCRVCERDNCPQRAFPAL
                     GRALDIDEHRSTVSPYLVQQS"
     gene            9478..10293
                     /locus_tag="VFC01_32385"
     CDS             9478..10293
                     /locus_tag="VFC01_32385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HZN84355.1"
                     /translation="MAGIAGSTGLAKVMMPVPDPHPDVFDREWPLRVADIDREGRLRF
                     DAATRHIQDIGSDQLREMGFEETHPLWIVRRTMIDLIKPVEFQDMLRMRRWCSGTSNR
                     WCEMRVRIDGRKGGLMESEAFWININRETQGPARISDDFIEGLQRTTNVDRLRWKAYL
                     KAGSREDAAEIREYPVRVSDIDIFDHMNNSVYWGVIEDYLFSTPELLHKPIRVTIEHD
                     AAVALGDKLEIISHVHPAGSTDKFGAELTDRTVRTLTYAVGEETKAVAAIFAL"
     gene            10559..11845
                     /gene="aceA"
                     /locus_tag="VFC01_32390"
     CDS             10559..11845
                     /gene="aceA"
                     /locus_tag="VFC01_32390"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HZN84356.1"
                     /translation="MSTVGQPKTPEEIQKDWDTNPRWKGITRTYTPADVVALQGSVVE
                     EATLARRGAEVLWNQLHDMEFVNSLGALTGNMAVQQVRAGLKAIYLSGWQVAGDANLS
                     GHTYPDQSLYPANSVPQVVRRINNALLRADQIAKVEGDTSVENWLAPIVADGEAGFGG
                     ALNVYELQKAMIAAGVAGSHWEDQLASEKKCGHLGGKVLIPTQQHIRTLTSARLAADV
                     ADAPTLVIARTDAEAATLITSDVDERDQPFITGERTAEGFYRVKNGLEPCIARAKAYA
                     PYSDLIWMETGTPDLELAAKFAEGVKSEFPDQMLAYNCSPSFNWRKHLDDATIAKFQK
                     ELGAMGFKFQFITLAGFHALNYSMFDLAYGYARNQMSAYVELQEREFDAEERGYTATK
                     HQREVGAGYFDRIATTVDPTSSTTALAGSTEEGQFH"
     gene            11925..12785
                     /locus_tag="VFC01_32395"
     CDS             11925..12785
                     /locus_tag="VFC01_32395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HZN84357.1"
                     /translation="MTNSIERVGVIGAGQMGAGIAEVSVRAGVEVLVYEPTEELTTAG
                     KTRITKSLERGVSTGKITERERDQALANLKFTTELKDLADRQLVIEAVIEDEAVKGEI
                     FAELDEIIADRDAVLASNTSSIPIMKIAAATKNPSRVLGLHFFNPVPVLPLVELVNTL
                     VTSDAAIARTEKFASDVLGKQVVRCSDRSGFVVNALLVPYLLSAIRMVEAGVATVEDV
                     DKAVVAGLSHPMGPLRLSDLIGLDTMKLIADSMFEELKDPHYAPPPLLLRMVEAGLLG
                     KKSGQGFYKY"
     gene            complement(12856..13716)
                     /locus_tag="VFC01_32400"
     CDS             complement(12856..13716)
                     /locus_tag="VFC01_32400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84358.1"
                     /translation="MVRPRLIVATEAIASGDLTRRDLMRSYTKIHRNVYAPKGIALTA
                     LDRAHAAWLWSERKAILAGHSAAALLGSKWLPPDSPGELARVRRPAPQGIRVYSGEIR
                     EDEICDVGGINCTTVARTAYDLGRRLPLETGVIRIDALLNATNVPVHQVYSVAQRYPG
                     ARGICRFRETLDLVDGGAESPQETRLRLLLVQSGISRPVTQIPVANPKGRKRRIDMGW
                     PEWMVGAEYDGEHHWTNPDDHAEDIERLEFLAAKGWLIVRVSARQLRYQRPEVVQRVR
                     DALIRRGCPL"
     gene            complement(13848..14693)
                     /locus_tag="VFC01_32405"
     CDS             complement(13848..14693)
                     /locus_tag="VFC01_32405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HZN84359.1"
                     /translation="MSKSADLPKLLKFRPGDKVADIDARATPGFKGAKADGVKLLDER
                     IGRLAELQEMLYANHKAADDTRSVLLVLQGMDAAGKGGIVKHVVGSVNPMGVRYTAFG
                     VPTEEERKHNYLWRIRRALPPAGHIGVFDRSHYEDVLIVRVHNLVPPEVWGKRYDEIN
                     RFEQKLVDDGTTIIKVAMFVSPDEQKKQLLERLEDPTKYWKYNPGDIDERKQWPKYQE
                     AYQTVLDRTSTEYAPWYVVPCDRRWYSRLAVTELLLHALEGLKLSWPPADFDVEAEKK
                     RLAEA"
     gene            complement(14686..15396)
                     /locus_tag="VFC01_32410"
     CDS             complement(14686..15396)
                     /locus_tag="VFC01_32410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN84360.1"
                     /translation="MAHQTSPVAVKTDGRKRRWHQHKVERRNELVDGTLEAIRRRGSN
                     VSMDEIASEIGVSKTVLYRYFVDKNDLTTAVMMRFAQTTLIPNMAAALSSNLDGFELT
                     REIIRVYVETVAAEPEPYQFVMANNSASKSKAVATSEQIIARMLAVMLRRRMVEVGMD
                     TGGVEPWAYHTVGGVQLATHSWMNDRRMSADDLIDYLTMLSWNALRGIVEVGGSLEKF
                     REQPHPSPVLPPQLLAHE"
     gene            15501..16832
                     /locus_tag="VFC01_32415"
     CDS             15501..16832
                     /locus_tag="VFC01_32415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HZN84361.1"
                     /translation="MAHRQDVGTRALPTAPRPSFAESLAGADTVADAERRRGLRRMKL
                     VALGFLVGATVVFLLCTWAQSNGAAAWVGYVRAAAEAGMVGALADWFAVTALFKHPLG
                     IPIPHTAIIKRKKDQLGEGLGAFVRENFMSPQVVETKLRDAQVAGRLGKWLSEPQHAQ
                     RVATETSNVLRVLVEMLRDEDVQHVLDRMIVKRVAEPQWGPPIGRVLATLLAERRQEA
                     LLQLLADRAFQWSLNAGDIIERVVERDSPTWSPRWVDHLVGDRIHRELMDFTDKVRRN
                     PDHELRRSATRFLFEFADDLQHDEATIQRAENVKEQLMARDEVAKAAETAWAAAKRII
                     LESVDDPSSALRTRIADSVVHIGESLRDDADLRDKVDNWIIRAAQHLVAEYGVEITAI
                     ITETIERWDADEASRRIELHVGRDLQFIRINGTVVGSLAGLIIYSVAQLLF"
     gene            16927..17385
                     /locus_tag="VFC01_32420"
     CDS             16927..17385
                     /locus_tag="VFC01_32420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN84362.1"
                     /translation="MPQDENLAAVVSNAAQNAAQDIGSFIRTQREAAQVSVRQLAEKA
                     GVSNPYLSQIERGLRKPSADVLQQIAKALRVSAEVLYVRAGILEPSEKSEVRDAIVTD
                     TAITERQKQVLLDIYTSFCQQNEAVSEEPTTEATTDQSPTLEIQQFNESP"
     gene            17399..18004
                     /locus_tag="VFC01_32425"
     CDS             17399..18004
                     /locus_tag="VFC01_32425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin-binding hemagglutinin"
                     /protein_id="HZN84363.1"
                     /translation="MAKKTPAKNQPTVDDLKAPLLAAVGAADLALERVNEIVATLRER
                     AGEARTDTQTRVEESRARLTKLQEDLPTQFDELRGRLSSEELRKFAETYADAAQTTYN
                     KLVERGEAALERIRTTPALEEGRERVERYSDQAVELTQEALGNVATQTRAVGERAAKL
                     VGVELPKKAAKAAPAKKAPAKKTAAKKAPAKKAPAKKVTQK"
     gene            18076..18357
                     /locus_tag="VFC01_32430"
     CDS             18076..18357
                     /locus_tag="VFC01_32430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2516 family protein"
                     /protein_id="HZN84364.1"
                     /translation="MELQGLVGYVLFVLQIAVVVTAVYAFVHAALQRPDAYTAADKLT
                     KPVWLVILGIGVLLALVLGITGVAIAAVAAGVYLVDVRPKILEIQGKSR"
     gene            18362..18772
                     /locus_tag="VFC01_32435"
     CDS             18362..18772
                     /locus_tag="VFC01_32435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2599 domain-containing protein"
                     /protein_id="HZN84365.1"
                     /translation="MRIVFAAATAAVTLALIVAPVASADPVPGPPYVDHVQWSKWADL
                     SSLRVYPTDAARQASLDPGTDAQAGEAWTEVLTLSPDADIPGMQAQFLCHWHYAELVE
                     PGKVSWNLEPWRNEVSEEQMVAAGCNPGGTEEPF"
     gene            18772..19452
                     /gene="deoC"
                     /locus_tag="VFC01_32440"
     CDS             18772..19452
                     /gene="deoC"
                     /locus_tag="VFC01_32440"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HZN84366.1"
                     /translation="MRRYSRAGVAALVDHTLLKPEATEADIVALLHEADELDVFAVCV
                     SPTMVATAKSFKTGDYHIASVVGFPSGKHVSAIKAEEARLAVAAGADEIDMVIDVGSA
                     LEGDFDAVKADISAVFDAIPSHAILKVIVESAALLALADEGRLADVCRIAEDAGADFV
                     KTSTGFHPSGGASVRAVEVMARTVGERLGVKASGGIRTADDAVAMLNAGATRLGLSGT
                     RAVLDGLD"
     gene            complement(19449..20342)
                     /locus_tag="VFC01_32445"
     CDS             complement(19449..20342)
                     /locus_tag="VFC01_32445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HZN84367.1"
                     /translation="MTDPWARPANQGPPVAPPPQFPQGPPPGQPAPPSAAPPQAAPPQ
                     KDSSLPGKVKNLFRDPLSIVLVVVIVIALVAAGLLAGELYARSRADSVVAKATECVTQ
                     DSASVSFGALPPFLMQHVSGHYTNIHIETAGNQIRQAKGMKVNVVIKDVRLEDTATSG
                     GSIGSLVANVTWSSQGIKETIQNAIPLVGSFVTGVTTNPSAGTIELQGALGNITAKPA
                     VVDNGISLQVTELTGLGFALPRETVQPALDAFSAQLTKNYPLGIHADSVQVTDTGVVS
                     QFSTQNATIPKQNEDPCFANL"
     gene            complement(20339..21154)
                     /locus_tag="VFC01_32450"
     CDS             complement(20339..21154)
                     /locus_tag="VFC01_32450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZN84368.1"
                     /translation="MRIALAQIQSGAEPTANLGLVEDYTRRAADAGASLVLFPEATMC
                     RFGVPLAPIAEPLDGSWASAVRAIAERAGIVVVAGMFVPADDGRVTNTLIATGPGIDT
                     HYDKIHLYDAFGFAESDTVAPGHEPAMITVGGVNVGITLCYDVRFPELYVELAQRGAQ
                     LITVHASWGTGPGKLEQWTLLARARAIDATGIVAAVDQAYPGDELASAGPVGVGGSLV
                     ASAVGEVLASAGADPQLLVSDLDLDAAQKARETIAVLHNRSEFTRVDKAESLR"
     gene            complement(21159..21950)
                     /locus_tag="VFC01_32455"
     CDS             complement(21159..21950)
                     /locus_tag="VFC01_32455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014876910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN84369.1"
                     /translation="MTGFPSTTRPVGAITRGTTGHNRLRRSDRWLVHSRRVRTALQAA
                     ADPLVVDLGYGALPVTTLELAARLRVVRADVRVVGLEIHPERIVPPRDGVEFALGGFE
                     LAGRRPVLVRAFNVLRQYPVEDVPAAWSVMQAALAPGGLIVDGTCDELGRRCCWVLLD
                     VDGPISLTLACDPFAIERPSDLAERLPKVLIHHIVAGQPVHTLLMSADRAWASVAGHG
                     VFGPRVRWRAMLDLLRDEGVPVEPPRRRMRDGVLTVPWSMVAPLG"
     gene            complement(21925..22440)
                     /locus_tag="VFC01_32460"
     CDS             complement(21925..22440)
                     /locus_tag="VFC01_32460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HZN84370.1"
                     /translation="MSRRMDYTIEFDAPTEKIYQDFTNREYWQTLMDSYRFLTPQSQI
                     RSFTSTERGTDIVFVQNLPRMYLPPIARTVVPVDMIITRKQHFDAYDHDKNRAKGTYS
                     ASIPHGPGSFGGQYFLTETDAGSKLRLASICKVHIPLVGGVLEDLILHHIKQLFDAEE
                     AFTADWISKHH"
     gene            complement(22463..22972)
                     /locus_tag="VFC01_32465"
     CDS             complement(22463..22972)
                     /locus_tag="VFC01_32465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HZN84371.1"
                     /translation="MPRSFDMATEYGGSVEQVHLAFGDEQYWLARLADSGADDYALDF
                     LTVEGDGIDIKTTQRLRADRLPAVVTQFHRGDLVLVREEKWSPIRDGQSTATLDLAII
                     DAPATLTGSAVLVPAKSGGGSRLEFNATVQVNVPLVGGKIENFIGGQLVDLLIAEQRF
                     TTVWITENA"
     gene            22999..24045
                     /locus_tag="VFC01_32470"
     CDS             22999..24045
                     /locus_tag="VFC01_32470"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZN84372.1"
                     /translation="MTGLDLGGAAVADDVPLAPLTTLRIGPVARRLITCTSTEQILAA
                     LRALDDVLVLAGGSNVVLADDLEDLTVVLLANTEITVEGNIVRAEAGAVWDDVVVTSL
                     AHGLGGLECLSGIPGSVGATPVQNVGAYGAEVADTIRRVRLLDRHTGEDRWVSPDALQ
                     FGYRTSILRHSAAAIVLEVEFALDADGRSAPLRYGELAGALDAEPGSRADPARVREAV
                     LSLRTRKGMVLDDADHDTWSVGSFFTNPVVSTDEFDRVARRTDGPVPNYPAPNGVKLA
                     AGWLVERAGFGKGYPGDGARARLSTKHALALTNRGDATTEDVIALARTVRDGVRTTFG
                     IDLTPEPILIGAAL"
     gene            24061..>25235
                     /locus_tag="VFC01_32475"
     CDS             24061..>25235
                     /locus_tag="VFC01_32475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZN84373.1"
                     /translation="MSPADPLPSMNRRRILTALAVGVVAPGALAACGSRISKPSADAP
                     AAPAGPSISYQPANATTDVAPVTPVRVEVKDGWFQRVALTNPDGKVVAGALNRDRTVF
                     NTTEPLGYGVSYTWAGSVVGHDGKAVPVEGNFTTINPSTQVNGQFQLADGQTVGVAAP
                     VIIQFDASITDKASVEKALKVTTEPPVEGSWAWLPDEVGGSRAHYRTKEYYPPGTKVS
                     VDAKLYGVPFGDGAYGAQDISLNFAIGRRQVVKAEASTHRIQVLDEQGAVIMDFPCSY
                     GEADLPRNITRSGIHVVTEKYEDFYMTNPAGGYSNAHERFAVRISNNGEFIHANPASS
                     GAQGNTNVTNGCINLSTSDAQQYFNSAIYGDPVEVTGTSIELSYSDGDLWDWAVSWD"
BASE COUNT         4428 a         8195 c         8244 g         4368 t
ORIGIN      
        1 tgcagatcac cgagcgcaag gccatcggga tcgacgacta catgcgcacc aacgttccgc
       61 acatctacgc gatcggcgac gtcaccggca agctgcagct ggcccacgtc gccgaggcgc
      121 agggtgtggt ggccgccgaa accatcggtg gcgcagacac tttgccgctt ggtgactatc
      181 ggatgatgcc gcgtgcgacg ttctgccagc cgcaggtggc cagcttcggt ctcaccgagg
      241 agcaggcacg cgacgagggc tacgacgtca aggtcgccaa gttcccgttc accgccaacg
      301 ccaaggcgca cggcatgggc gacccgagtg gcttcgtgaa gctgatcgcc gacggcaagt
      361 acggcgagct gatcggcggc cacctcatcg gccatgacgt gtcggagctg ctgcccgagc
      421 tgacccttgc gcagaaatgg gacctgacag ccaacgagct ggcccgaaac gtccacaccc
      481 acccgacgat gtcggaggca ctgcaggaat gcttccacgg cctcgtcggc cacatgatca
      541 acttttgaga gcgatctggg tcgccggcat cggcggattg gtggtcggcc acatcctgtg
      601 gctggccggg atctcggtcg cgatcgcgac acgaacgatc aacagctggg tgctgatcgt
      661 cgctggggtg tcgtttgtcg ccggcgccat cgccgcgtat ttcggctggc ggtcgtatca
      721 gcgaaagtcg gagatatggg ccgcgttcct gtgggccctg ccgatctcgc ccgtgttgtt
      781 gtcgctgacg gtgctcggcg tcacgtatct gtaggtttgc cgacgacacg cgcgcagatg
      841 ctggccttga tgcgttgacg cgcttacttt cgaccgatga aatctgttgc ggtgttgggg
      901 ggcggtgtcg gcgggctgag cgcagcgcat gagctcgccg agcgagggct gaaggtcacc
      961 gtctacgaga tgcgagacgc gttcggcggc aaggcccgat cgatgcccgt gcctggctcg
     1021 ggtaccggag ggcgcaagga ccttccgggc gagcatggat tccggttctt ccccagcttc
     1081 tacaaacaca tcatcgacac gatgggacgc atcccctacg ggcagggaac tgtcgccggc
     1141 aatctcatcg agaccaccaa gatattgctg gcgcagggcg gagaccggaa tgagctgccc
     1201 gccccgacgg ttccgccgtc gtctgtcaac gatgtggtgg ccgcgctgaa gttcatgcgc
     1261 gagttcgtgt tcggtctcgg catcccgatc agtgaattcc tggactacac caagggcttg
     1321 ctgacgctgc tcgtttcctg cgacgggcga cgctatggcg agtgggaaga gatgagttgg
     1381 tgggacttca tggatgccgg gcaccgcact gaggctttca ataagtttct cggcgacggg
     1441 atcacgcgct gtttggtagc cgcgcgcgga cgtgaaatgt cggcgcgcac tggtggaatg
     1501 atctcgttgc ggttgatgct caactcgggt gcgccggtag accgggtgct caaaggcccg
     1561 acgagcgagg tgtggatcga cccgtgggtc gagcatctgc gcggcttggg cgtcgagttg
     1621 cgcggcgggc acacggtcgg cggcatccac tgcgacgggc gggagatcac cggcgtcacc
     1681 gtcaccggtc cccacggtga cgaactgatc accgccgact actacatcgc ggcgatgccg
     1741 aaggagaagc tggaactgtt gatctccccg cagatgtgcg ccgccgatcc gcacctcgct
     1801 gcgctgggcc gactgcagac ccggtggatg aacggcgcga tgttctatct ggatacagac
     1861 gtgccgctgg agcacggtca cattatcttc atcgactccg agtggtcgct cacggcgatc
     1921 tcgcaggcac agttctggaa gcgcgttgtc gacctcaagc agcgtggtga cggaagggtg
     1981 gaaggcattc tgtccgttga tgtttcagag tggcggaccc ctggaagcac cggcaaggtc
     2041 gccagcgcct gcacaaaaga tgagatttgc gacgaggtgt tgaaacaaat gcaggaccac
     2101 atcaaggata ggaagctgga caaggccaat gtcgttacgt gctttctcga tccggcgatc
     2161 tcgtttccca acccgtcgca agcggccaac gccgagccat tgctcatcaa caccccgggg
     2221 tcatgggcgg atcggccgga agcctggacg gcaatcccga acttctttat tgccgccgac
     2281 ttcgtgcgca ccgataccga cttggcgacg atggaagccg cgaacgaagc agcccgcctc
     2341 gcggtcaacg caatcctcaa ggcgacgggg tcgaaggagg gatgtgagct ctggaagttt
     2401 ccggagccat gtctccttgc gccgtttcgc ctcatggacc ggctgctgtg gcggctcggt
     2461 cgaaagcctc cgaagctacc cgtgcgcatg acgtcgacgg gggaagtgga accgaccggt
     2521 ccagtcgcgc gcacagtact ggcggttacg cgccggctac gttaacttct tctgctgcaa
     2581 cggcatgggg atccctaggt cgcggtggcc actgcacgct cgctcagttg tctagcgttt
     2641 catcgcgcgg cgcgagttcg agcatccgtc gccccaacat ggcgcgcgag cggacgccca
     2701 gcttgcggta gacgcgggtc agaacgctct cgacagtctt ggcgctgacg tagaccgccg
     2761 cggcgatctc gcggttactc agcccctcgg cggccaactc cgcgacccgc tgttcgctcg
     2821 gtgtcaggtc gaccccgtgg cccgggcgga cgttgacgcg tgcacgctcg gcatgaaccc
     2881 gtgccgccca cagcgggttg tccagctgct cgaaggtccg cagggcttgg tcgagcgcag
     2941 cagcggcagc ctgcttctgg cgcagccggc gatacagttg tccgagcagg agttgggtgc
     3001 gggcatgctc gagtggcatc cgcagccccc ggtgcgcatc cgccgcctga gccacgactc
     3061 gaaccgccgc ctcgacgtcg ccggatgcgg ccagccacat gccgcggcac cgtgcggcaa
     3121 cgagcaccat ccacgcacgg ccgagttcgc ggccgtcgcg ctcgagcctg tcgatcaaca
     3181 cttcggcctc ctcgaagcga ccgctgatca ccatcgcctc gatggcgtcg ggaaggtatt
     3241 ccatcttgga gatctcggtg ttgggcaccg tgtcggcgac cgccagcagc ggcgccagga
     3301 cgttcaaagc ctcggcgtgt ctgcctgccg atacctccaa gcgggcaagg cccgtcagcg
     3361 ccaaatcgac ccaccacgag gatgattcgc tgcgcgctgc agattcgagg gccgcagtca
     3421 gatctgcacg ggcttcttgc tcgtgtccgg cgtgcaccgc cacccaaccg cgcatggcca
     3481 gcccgagacc gcgtatcgcg gaaccgccga tctcttcgac ccgtcggaac gtgtcctcgg
     3541 cgaagtgtcg tgcctcgaca tagcgtccca gccaaacatt caccagcaca ctgttgtagg
     3601 ccacaaacaa catctcgatt tcgccgccgc ggcgcaccag ctgcgtagtc aatgcgtcca
     3661 aagcggtcgc tgattcctcg agccggccgg tccacgccat caacaacgcg tcaaccgcgc
     3721 tcgcgcgaaa cggtattgga acgtcaccgg cgtggtcttc gagttcgagc gcgcgcgtca
     3781 tggcggtctc gtcgtatccc gaaccgcagt ggaagctgac gtaggcccac gacgccagcg
     3841 cttggctgat cagcacaggc tgcgccgccg cttcggcctc ggccagcgca cgccgggcat
     3901 gctgaggcgc agtctcgtag atgccctcgt tgatttgagc gaatgcgagc atcatcagca
     3961 cctcgaccag tacggtcggg tcgcccgtcg cgtcgtcgat cgcctcgtaa agcacgtcgg
     4021 cggcctgtga atagccttcg gtgtgcatga ggatcacggc gtacccgaac atcatcaacg
     4081 cccggaaggg gccaggttcc atctggtcta tcccgggctt gagcacttcc tgtgcgcggg
     4141 cagcgtcgcc ggagcgcaga tggtgctcgg ccgccgcgaa ccgccgcatc ggggtgtcgc
     4201 caccgagacc aatggcgaga tcgagcagtt cagcggccgc tgccggggct ccgcgccctc
     4261 ggaccaccgc ggccgcggcg tcgagtgccg ccagagtttc ctgatcgctg ctggtggcgg
     4321 ccagcgccag atgccgcgct ttggattccg gctcgctcag cgcctccgac caggctcgat
     4381 gccagtagcg gcgccgcgcg ggcgcggcgt tggcatagac cccccgggcc aacacgggat
     4441 gggtgaacga cacgcggttg ccgtcaagcg cgatgatgcc tgcgctttcg ggttcctcca
     4501 gcagctcgac gacctcctcg acggtcgtgc cggtcacacc gaccagcatg tcgactgtcg
     4561 gctgcggcag acacgctgcc gccaccaaaa cgtcgagagc gcggccgtcc aggcgagcga
     4621 tccgcatccg caccagattg gcgagactgc gcgggaaaac ctggtgttcg ccgccgccct
     4681 cggcgtcgat cgcacgggct agttccaatg cgtagagcgg gttgccgccg gagacttcgg
     4741 cgatgcgcac catcgtcgga tgcggaaacg agcgaccgat tctgttcttg atcaaggcat
     4801 ggagctcatg cagacccaaa ggcgacactc gcactttccc tatttcgtcg ccggtggcca
     4861 gctgcagcca ggacagcgcg ttgacagtgt cgtgttcttc gcgctcggtg aacaagacgc
     4921 cgacgcggtt cttgagccgg cgcaccgcaa acgccatcac cgcccgactc gaagaatcca
     4981 gccaatgcac gtcatcgacc gcaatcaatg gcggagcgtt ggccgacaat gcctctatta
     5041 cggacatgaa tgccgcggcg accatctgct gattggcttc cggaccctcg ccacttgccc
     5101 gcatcagcac gcggtcgagc gctcggtgct gcaggtccgg gatgcgggcc atgagcgtcg
     5161 gttctacatc ggcgacaagg tctccgaccg tcgcgtaggc gtgcaccgtc tctgcctggc
     5221 tcgcgcgcgc ggtcaacaca cgaaacccgc gctcgcgcgc ccgatcaacg gcgaactgcc
     5281 aaaaggtcgt cttgccgatg ccggcctcgc cctgaatgag cagagccgac ggccgtgtct
     5341 tcgccgcggt cagaaggtct ccgaccgcgg cgtcgtccag cgaaggtccc gacaccggca
     5401 ttctgcgatt atggcaatca gcaaacccgc ggaacacgca gaccgaggag acacgccaga
     5461 attagccagc cattcattga gttaagtccc ccgcggagcg ggcccctagc gaccaaattt
     5521 cgctaggtag ctgtctacct ataggcagac gatgacggca gcgcgacggt ggcttctgtt
     5581 ggccaacgga gttttcctgg tcgtggtcgg cggtgtccaa gtggtcttcg aactgctcgc
     5641 ctactacaag ggcgtcgggc cgtacggcta catcttcgag gagtcgccct acgccatcgg
     5701 cggggtggaa gcgcacggac tggccttcat catcggcgtg ctgctgttgg cggtagccgt
     5761 tcgggacctg cgacggttct ggcacatcct ttccgcagtg gtgcacacgc tgctcggcgc
     5821 ggcgaatctt gttttctgga acagctttat cgtgttcggc atggtgccga tgggggcggc
     5881 ggccacggtg gtgcacttcg tgctcgtggc cgcgcacgtc gccgcttttg tggtgtctcg
     5941 tgggactgct cgagagcgtt gagcggatct accaccggtt cgccgcgtac ccgtgcccgg
     6001 cgaacctgtg ggtatgcgag caatgcggcc cggaatggtc gaccgaggac atccgcgcct
     6061 taccgttgcg gtcggtgtcc tccctgccac agctcgtcgc ggtccatgtc atgtctctcg
     6121 atgtggacgg tttacggcat ttcttcccgc ggttgatgga gttgatgctg cagaaaccaa
     6181 gtccggtgtt cgacttccgg ctggccgact tgaaggaccg gctgcgcggt tggcggcccg
     6241 aggaagtcgg gtgtgtccga gacctcgccg acgccgtttg ggccgagctg ctggtcggct
     6301 acccacccgc gctcggatac ttctcggatt gcccgtcggc gctcgatctc ctcgactggt
     6361 gcgagctgcc cctcgtgaag tacctcggtt tgttgctgac cgtcgatggt ttgcccgccg
     6421 caaggcacgt cgccgatctc atcgacgcgg tgttcacgat gcgagagccg ttcgaatctg
     6481 cttctaagtc aacggtttcc gagtggatca cagatccctc ggtcggcgag cgtctgcagg
     6541 cggcgttctt cacggcgcct atcgaggagg cagcgtggca gctttccgcc gcccacgagc
     6601 tgtggactgt ttgtgcgcgg tagcggtcgg gagcccgctg cgttatcgtc tgctgcaccg
     6661 cagacgacct tggaggaagc tacgtggcag cgtcgaagta cgccgtaatc tcttttgccg
     6721 gtgcagtcct ggccgggtgt gcgttggctc tggcggccaa tgcccaggcc gacgacaact
     6781 gtgatccgtt cctgctgtcg atgactccgc agcctgttct cgcatgtcag gctcccgacg
     6841 tcgcaccgcc gcccgaccaa cctgcggtcg ctccggtgaa cgacgtcgat gtgccgctgc
     6901 ctgccgaggt gcctcccccc gcggagcccg ggccgttcgg cgacgtccag gctcctgcgc
     6961 cgcttctgtt gccggacggg tctgtccgct agcgggctcc ttcgacgccc ataccgtagg
     7021 gcatccgcgc aggccgcgcg tggtctacaa gatcagacgc agtcggtgca gattagttgg
     7081 ccatctttgc ggagagccaa ccgagcgtgg tgctgcacca ggaagcagct cgagcagacg
     7141 aattcgtcgg cctgcttggg gactacgcgg acggtgagct cctcaccggt gaggtcggcg
     7201 tcggggaggt cgaacgaatc gaccgcgtcg ccgtcgtcga catcgataac cgcggtcgcc
     7261 gcgtcgttct tgcggcggct ggtcagctcc tcaagtgatt cgtcggcatc gggctcggtg
     7321 acgcggggag cgtcatagtc agtggccatg cggcatgccc cttcctaatc aagtctggta
     7381 aatcgccaaa cgcagacagg aagcggattg ttccctacgt cagtcaggaa agtccagcgg
     7441 aattttcagc gtcgccatgg tggcggcaag ggcgtcgtat tgcccagcaa gagtgcagaa
     7501 ttcgatgagc tgcgcccggt tgtagatgcc cgacagcgcc gcccacgttt cgggtgacat
     7561 cgagcgggtg atgacgaact cgtcggtcgc ggtgatcagc acccgctgcc ggtcgttgag
     7621 cccgtcggcg tccgggccct cgaagatcct ggcctgcagt tcggcgtcga cgccacggct
     7681 cttcgccagc cgccgatgct gttgcagctc gtactcgcag tcgcgcaggt gtcccacccg
     7741 aaggatgacc agttcggcgt ccttgcgcga gagcttgccc gcatacagca gataacccga
     7801 gaacggcagc cacgcgagga acagcagttt gtgctgtcca agcacattga acaggtgaaa
     7861 cttcggtgcg cggatgccgc gggcgccgag cttggcgatc gcccagttga tcggcccgag
     7921 ctccttgaaa ccgcccggcg ggatacgtgc gggcgcgctc atgactgctg caccagatag
     7981 ggcgacaccg tgctgcggtg ctcgtcgatg tcaagagcgc ggcccagcgc ggggaacgcc
     8041 cgctgcgggc agttgtcgcg ttcgcaaact cggcagccgg cgccgatcgg cgtcgcgttg
     8101 tcacccgaca agtccagtcc ctccgaatag accagccgat gcgcgtgccg gagttcacag
     8161 cccaggccga tcgcgaacgt cttccccggc tgaccatagc gcgacgcccg gcgctccact
     8221 gtgcgcgcca cccacatgta gttgcgtccg tcgggcatct gcgcgacctg caccaggatc
     8281 ttgcccgggt tggcgaacgt ctcgtagacg ttccacagcg ggcaggtacc acccgacgac
     8341 gagaagtgaa aaccggtggc ggactgccgc ttcgacatgt tgccggcccg gtcgacgcgg
     8401 atgaacgaca acggcacacc acgcatcgac ggccgttgca gcgtcgacaa ccgatgcgcg
     8461 atggtctcat agctgacgga gtagaacgcc gacagccgct cgatgtcgta gcggaagttc
     8521 tcggccacgt cgtgaaactg ccgatacggc aacaccgttg cggccgcgaa gtaattggcc
     8581 agccctagcc gggccagggt cgtcgactcc tcgctggtga acttgccctc gtcgaccagc
     8641 ttgtcgatca ggtcgccgaa ctcgaggtag gccagttcgg cggccatttt gaacaccgtc
     8701 tgtcccgacg agaggtgacc gctgatctcc agggtcttgg tagtggggtc gaagcggtgc
     8761 agcaccgtgt cgccgaggtc gatgcgccga atgatgtgca ccccgtgcac cctggtcagc
     8821 cggtcggaga gctcgcgagc gagttcggcg cggtgcatgc gcatccggat ggtgaggtcc
     8881 tcggcggcgg tgtcgagctc gtgcagatag ttctgccgtt cgtagaagta gtcgcgcact
     8941 tcctcgtgag gcatcgtgat cgacccggtg ccgctgccgt cggagttcct gtcctcggtc
     9001 gcggcggcca gctgcgtggt ggtcagccgg tagcggcggt gcaggttgac catcgcacgg
     9061 gccaacgccg ggtgtgagcc gaccatgtcg gcgatctcgg ggaggtcgac gtcgatgtcg
     9121 aggtcgcggt ccatcgtgac ctcgcgaagc tcggcgacca ggcgggtgtc gtcctgggat
     9181 gcgaaaaacg ttgcgtcgac accgaacacc tcggtgatgc gcagcagcac ggcgacggtc
     9241 agcggacgca cgtcgtgctc gatctggttg aggtagctcg gtgagatctc cagcatctgg
     9301 gcgagtgctg cctggctgaa gccgcgttcg ctgcgcagtt gtcgcacccg ggagccgaca
     9361 aacgttttcg ccacgtcaga cagcctaaca acgctgcgaa gccagccttc gcattgttgg
     9421 cagattgcgg gctatggcag gatcgggccg gttggagtaa caggggagat cacggacatg
     9481 gcgggaatcg caggtagcac cggtttggcg aaggtgatga tgcctgtacc cgatccgcac
     9541 cccgacgtct tcgacaggga gtggccgctg cgggtcgccg acatcgaccg cgagggcagg
     9601 ctgcgattcg acgcggccac ccgtcacatc caggacatcg gctcggacca actgcgcgag
     9661 atggggttcg aggaaaccca tccgctgtgg atcgtccggc gcacgatgat cgacctgatc
     9721 aagcccgtcg agttccagga catgctgcgg atgcggcggt ggtgctccgg cacatcgaac
     9781 cggtggtgcg agatgcgggt gcgcatcgac ggccgcaagg gcgggctgat ggagtccgag
     9841 gcgttctgga tcaacatcaa ccgcgagacc cagggcccgg cgcgcatctc cgacgacttc
     9901 atcgaaggcc tgcagcgcac gacgaacgtc gaccggctgc ggtggaaggc ttatctgaag
     9961 gccggcagtc gcgaggacgc cgccgagatc cgcgaatacc cggtgcgcgt cagcgacatc
    10021 gacatcttcg accacatgaa caactccgtg tactggggcg tcatcgagga ctatctcttc
    10081 agcacgccgg aactgctcca caagccgatc agggtgacca tcgagcacga tgccgcagtg
    10141 gcactgggcg acaagctgga gatcatttcg cacgttcacc cggcaggctc gaccgataaa
    10201 ttcggcgccg aactgacgga tcgcactgtt agaacgctca catacgcggt cggcgaggag
    10261 acaaaagccg tcgcagcgat cttcgccctc tgactggttc aggtttaaca gtacgagcgt
    10321 actgaccagc gaaaattgcg ttactggccg gtagctactg aaccggtaca gcggatagcc
    10381 atattcgcaa acttagcaag ttaacggtag gtgttggcga aagttggcaa gtgaaacgag
    10441 tggaccagcg gttatgcctt ggtgcattct cgacttagca cacaagtgaa ggtgctggag
    10501 cgttaacaac tgttgacggt tggagcgact ccagcgactc gcaagagaag gagcaccaat
    10561 gtcgaccgtt ggccagccga agaccccgga agagatccag aaggactggg acaccaatcc
    10621 gcggtggaag ggcatcaccc gcacctacac ccccgccgac gtggtggctc tgcagggttc
    10681 ggtcgtcgag gaggccacgc tggcccgccg cggcgccgag gtgctgtgga atcagctgca
    10741 cgacatggag ttcgtcaact cgctcggtgc gctgaccggc aacatggccg tgcagcaagt
    10801 gcgcgccggc ctgaaggcca tctacctgtc gggttggcag gtcgcgggcg acgcgaacct
    10861 gtccggccac acctacccgg accagagcct gtatccggcc aactcggtgc cgcaggtggt
    10921 gcgccgcatc aacaacgcgc tgctgcgcgc cgaccagatc gccaaggtcg agggcgacac
    10981 gtcggtggag aactggctgg ccccgatcgt cgccgacggt gaggccggct tcggtggcgc
    11041 gctcaacgtc tacgagttgc agaaggccat gatcgcggcc ggtgtcgcgg gctcgcactg
    11101 ggaggaccag ctggcgtcgg agaagaagtg tggtcacctc ggtggcaagg tgctgatccc
    11161 gacccagcag cacatccgca ccctgacctc ggcccgcctg gcggccgacg tcgccgacgc
    11221 gccgaccctc gtgatcgccc gtaccgacgc cgaggcggcg acgctgatca cctccgacgt
    11281 cgacgagcgc gatcagccgt tcatcacagg tgagcggacc gccgagggct tctaccgggt
    11341 gaagaacgga cttgagccgt gcatcgcccg cgccaaggcc tacgcgccgt actccgatct
    11401 gatctggatg gagaccggca cgccggacct ggagctggcg gcgaagttcg ccgagggcgt
    11461 caagagcgag ttcccggacc agatgctggc ctacaactgc tcgccgtcgt tcaactggcg
    11521 caagcacctc gacgacgcga cgatcgcgaa gttccagaag gagctcggcg cgatgggctt
    11581 caagttccag ttcatcacgc tggccggctt ccacgccctg aactactcga tgttcgatct
    11641 ggcctacggc tacgcccgca accagatgag cgcctacgtc gagctgcagg agcgcgagtt
    11701 cgacgccgag gagcggggtt acaccgcgac gaagcaccag cgcgaggttg gcgccggcta
    11761 cttcgaccgg atcgcgacca cggtggaccc gaccagctcg acgaccgcgc tcgccggttc
    11821 gaccgaagag ggtcagttcc actgatcctg tagggattga cagcgcaggc cccgcccaga
    11881 agtgttgggc ggggcctgac gcatgtgcga gaggaagttc agcagtgacc aattccattg
    11941 aacgagtagg cgtcatcggc gcgggccaga tgggcgcggg catcgccgag gtgtcggtgc
    12001 gggccggtgt tgaggtgctc gtgtatgagc ccaccgagga gctgacgacg gcgggcaaga
    12061 cgcggatcac caagtcgctc gagcgtggcg tgagcaccgg caagatcacc gagcgcgagc
    12121 gcgatcaggc gctggccaac ctgaaattca cgaccgagct caaggacttg gcggatcgcc
    12181 agctcgtcat cgaggcggtg atcgaggacg aagcggtcaa gggcgagatc ttcgccgagc
    12241 tcgacgagat catcgccgat cgcgacgcgg tgctggcgtc gaacacgtca agcatcccga
    12301 tcatgaaaat cgctgcggcg acgaagaatc cgagccgcgt gctgggtttg cacttcttca
    12361 atccggtgcc ggtgctgccg ctcgtcgaac tcgtcaacac gctcgtcacc tccgacgccg
    12421 cgatcgcacg tacggagaag ttcgccagtg atgtgctggg taagcaggtg gtgcgctgca
    12481 gcgaccgctc cgggttcgtc gtcaatgcgc tgctggtgcc gtatctgttg tcggcaattc
    12541 gcatggtcga ggcgggcgtc gcgaccgtcg aggacgtcga caaggccgtt gtcgcagggc
    12601 tgtcgcatcc gatgggacca ctgcggttat cggacctcat tggtctcgac accatgaagc
    12661 tgatcgccga ctcgatgttc gaagagctga aggacccgca ttacgcgccg ccgccgctac
    12721 tgctgcggat ggtggaggcc ggtctgctcg gcaagaagtc gggccagggc ttctacaagt
    12781 actgaccgag ctcccgtccg agcgcacggt tttgtactca aacgtcgagt gtgcccgcaa
    12841 acaacagtgc gctcgtcaga gcggacaccc tcggcggatc aacgcgtccc gtacccgttg
    12901 caccacctcc ggtcgttggt agcgcaactg cctggcgctg acacggacta tcagccaacc
    12961 ttttgcggcc aggaactcga ggcgctcgat gtcttcggcg tgatcgtcgg gattcgtcca
    13021 gtggtgctcg ccgtcgtact cggctccgac catccactcg ggccatccca tgtcgatgcg
    13081 acgctttcgg cccttcggat tggcgaccgg gatttgggtg acaggccgtg agatgcccga
    13141 ctgcacgaga agcagtcgca gccgcgtctc ctgcggtgac tcggcaccac cgtcgacgag
    13201 gtccaacgtc tcacggaacc tgcagatgcc gcgggcgcct ggatagcgtt gcgcgaccga
    13261 ataaacttgg tggacaggga cattcgttgc attgagtagt gcatcgatcc gaatgacacc
    13321 ggtttcgagt ggcaatcgcc gaccgaggtc gtaggcagtc cgggccaccg ttgtgcagtt
    13381 gatgccgccg acgtcacaga tttcatcttc gcggatttcg ccgctgtaca cccggattcc
    13441 ttgcggtgct ggccgtcgga cccgcgccaa ctccccgggc gagtcaggag gaagccactt
    13501 gctgccaagc agtgccgcag ccgaatgacc tgccagaatc gccttgcgtt ccgaccacag
    13561 ccatgcggcg tgcgctcgat cgagagctgt gagcgctatg cccttcggcg catagacatt
    13621 ccgatggatc tttgtgtagc tgcgcataag gtctcgtcgc gtcaggtcgc cgctggcgat
    13681 agcttcagtc gcaacgatga gacgtggtcg caccatctca tcgtcgggca accgcgggcg
    13741 tgcccctcac acaagttgtc gtggctgtgg ataacgaccg tcgtcgagcg cacgcttgtt
    13801 gtacagaaac gtcgggtaga cccgcaaata accgtacgct cggcgattca agcctcggcg
    13861 agccgcttct tctcggcctc gacgtcgaaa tcggctggcg gccaagacaa cttgagtccc
    13921 tcgagcgcgt ggagaagcag ctcggtaacc gccaaccggc tataccacct gcggtcgcac
    13981 ggcacgacgt accagggcgc gtattcagtc gacgtacggt caaggacggt ctggtaggcc
    14041 tcctggtact tcggccactg cttgcgctca tcgatgtcgc ccgggttgta cttccaatac
    14101 ttggtgggat cttccaggcg ctcgagcagc tgcttcttct gctcgtcggg cgagacgaac
    14161 atcgcaacct tgatgatcgt cgtgccatca tcgacgagct tctgctcaaa tctgttgatc
    14221 tcgtcgtagc gcttgcccca tacctcgggt ggcaccaagt tgtgcacccg cacaatcaag
    14281 acgtcctcgt agtgcgagcg gtcgaacacc ccgatatggc cggcgggcgg gagtgcgcgc
    14341 cgtatccgcc acaggtagtt gtgtttgcgc tcttcttcgg tcgggacgcc gaatgccgtg
    14401 tagcggacgc ccatcggatt caccgaaccc acaacatgtt tgacgatgcc gcccttgccc
    14461 gcggcgtcca tgccctgcaa caccagcagc accgaacggg tgtcgtcggc cgccttgtgg
    14521 tttgcgtaca gcatctcctg cagttcggcc agccggccga tgcgctcgtc cagcagtttc
    14581 acgccgtccg ccttggcgcc cttgaacccc ggcgtggcac gggcgtcgat gtcggccacc
    14641 ttgtcaccgg ggcgaaactt caacagcttg ggcagatctg cagacttact catgagccaa
    14701 caattgtggc ggcaacaccg gcgacgggtg cggctgttcg cggaacttct ccagcgatcc
    14761 gccgacctcg acgatgccgc gcagcgcgtt ccacgacagc atcgtcagat agtcgatcag
    14821 gtcgtcggcg ctcatccgtc ggtcgttcat ccacgagtgc gtggccagct gaaccccgcc
    14881 gacggtgtgg taggcccacg gctcgacgcc accggtgtcc atcccgacct cgaccatgcg
    14941 gcggcgcagc atcaccgcga gcatgcgcgc gatgatctgc tcggacgtag ccaccgcctt
    15001 gctcttgctc gccgagttgt tcgccatcac gaactggtag ggctccggct cggcggccac
    15061 ggtctcgaca tacacgcgga tgatttcgcg ggtcagttcg aagccgtcca agttcgacga
    15121 cagggccgcg gccatattcg ggatcagcgt ggtctgcgcg aaccgcatca tcacagcggt
    15181 cgtgaggtcg ttcttgtcga cgaagtaccg gtacagaacc gtcttcgaca ccccgatttc
    15241 ggaggcgatc tcgtccatgc tgacgttgct gccgcgccgt cggatggcct ccagggtgcc
    15301 gtctaccagc tcattacggc gctccacctt gtgttggtgc cagcgccgtt tgcgtccatc
    15361 tgtcttgacc gccaccggag aagtctgatg tgccactatc gccgagatcc gttccctagt
    15421 ccttcctgat actacggccg atcttgttct gaaggcttgc aggacccggc cccgacgccg
    15481 ttgtagcgga caatgtaggg gtggcacaca gacaggacgt cggcacgcgc gcgctgccga
    15541 cggcgccgag gcctagcttc gcggagtcgt tagcgggcgc ggacaccgtc gccgatgccg
    15601 agcgtcgccg cgggctgcgg cggatgaagc tcgtcgcgct gggcttcctg gtcggcgcca
    15661 ccgtggtttt cctgctctgc acctgggccc agtccaacgg agccgcggca tgggtgggct
    15721 acgtccgggc cgccgctgag gccggcatgg tgggcgcgct ggccgactgg ttcgcggtca
    15781 ccgccctatt caaacatcca ctcggcatcc cgatcccaca caccgcaatc atcaagcgca
    15841 agaaagacca gctcggcgag ggcctgggcg cctttgtgcg ggagaacttc atgtcgcccc
    15901 aagtcgtcga gaccaagctg cgcgacgccc aagtggcagg ccgactcggc aagtggctct
    15961 cggagccgca gcacgcgcag cgggtcgcca ccgagacgtc gaacgtcctg cgcgtcctgg
    16021 tggagatgct gcgcgacgag gacgtgcagc atgtgctgga ccggatgatc gtcaagcgtg
    16081 tcgccgaacc gcagtggggg ccgccgatcg gccgggtgct ggccacgctg ttggccgagc
    16141 gtcgccagga agcgctgcta cagctgctcg cggaccgggc ctttcagtgg tcgctcaacg
    16201 ccggcgacat catcgagcgc gtggtcgaac gcgactcgcc cacctggtcg ccgcgctggg
    16261 tcgaccacct cgttggcgac cgtatccacc gcgagctgat ggacttcacc gacaaggtgc
    16321 gccgcaaccc cgaccatgaa ctgcgcaggt cggccacccg cttcctgttc gaattcgccg
    16381 acgatctgca gcacgacgag gccacgattc agcgcgccga gaacgtcaag gaacagctga
    16441 tggcccgcga cgaggtcgcc aaggccgccg agaccgcgtg ggccgccgcc aagcggatca
    16501 tcctcgagtc ggtcgacgac ccatcgtcgg cgctgcgcac ccgcatcgcg gactcggtgg
    16561 tgcacatcgg cgagtcgctc cgagacgacg ccgacctgcg cgacaaggtc gacaactgga
    16621 tcatccgtgc agctcaacac ctggtcgccg aatatggggt ggaaatcaca gcgatcatca
    16681 ccgagacgat cgagcgctgg gacgccgacg aggccagtcg ccgcatcgaa ctgcatgtgg
    16741 gccgtgacct gcaattcatc cggatcaatg gcactgtggt ggggtcgctg gccggactga
    16801 tcatctattc ggtagcccag ctactcttct gacctgcgct aactagtgct tgcaaaagtt
    16861 agcaccccgt cgtacggtta attgcgtcgc acatcggata cccgacgcaa ccgagggggc
    16921 cacgccatgc cgcaggatga aaatcttgcc gccgtcgtgt ctaacgctgc ccagaacgcc
    16981 gcgcaggaca tcggcagttt catccgtacg caacgcgagg cagcacaggt atccgtgcgg
    17041 cagttggccg agaaggccgg cgtcagcaat ccctacctca gccagatcga gcggggattg
    17101 cggaaaccct cggccgacgt gctgcagcag atcgccaagg cgctacgggt gtccgcggaa
    17161 gttctctacg tgcgggccgg gatcctcgag cccagcgaga agagcgaagt ccgcgacgcc
    17221 atcgtcaccg acacggcgat caccgagcgg cagaagcaag tgctgctcga catttacacg
    17281 tcgttttgcc aacagaacga agccgtcagt gaggagccga cgactgaagc gactacagac
    17341 caatcaccca cgctagaaat ccagcaattc aacgaaagtc cctgaaagga atccaacaat
    17401 ggcgaagaaa acgcccgcca agaaccaacc caccgttgac gacctcaagg ccccgctgct
    17461 cgctgccgtg ggcgctgctg acctggccct cgagcgggtc aacgagatcg ttgccaccct
    17521 gcgtgagcgt gccggcgagg cccgcaccga cacccagacc cgcgtcgagg agagccgcgc
    17581 tcggctgacc aagctgcagg aggacctgcc cacgcagttc gacgagctcc gcggtcggct
    17641 gtcgtccgag gagctgcgca agttcgccga gacctacgcc gacgccgcgc agaccaccta
    17701 caacaagctc gtcgagcgtg gcgaggccgc cctcgagcgg attcgcacca cccccgcgct
    17761 ggaagagggc cgtgagcgcg tcgagcgcta ctcggaccag gccgtcgagc tgacccagga
    17821 ggcgctgggc aacgtggcga cgcagacccg tgcggtcggc gagcgtgccg ccaagctggt
    17881 cggcgtcgag ctgccgaaga aggccgccaa ggccgctccg gccaagaagg ccccggctaa
    17941 gaagaccgcc gccaagaagg cgccggccaa gaaggccccg gccaagaagg tcacccagaa
    18001 gtaaataagg gctcaggtca cccctcgggg tgactcagag tcgacccggg gacgtaaccc
    18061 gcttaggcta gctatgtgga gcttcaaggc ctggtgggtt acgtcctctt cgttttgcag
    18121 atcgcggtcg tggtgacggc ggtctacgcg ttcgttcacg ccgcgctgca gcgccctgac
    18181 gcctacactg ccgccgacaa gctgaccaag ccggtgtggc tggtgatcct gggcatcggc
    18241 gtgctgctcg ccttggtgct cggtattacg ggcgtggcga tcgccgctgt ggcagcgggc
    18301 gtctatctcg tcgacgtccg gcccaagatc ctggaaatcc aaggcaagtc gcgctagccg
    18361 cgtgcgcatt gtcttcgccg ccgcgacggc ggctgtcacc ctggccctca tcgtcgcgcc
    18421 cgtcgcgtct gccgatccgg tgcccggccc gccgtatgtc gaccatgtcc agtggtcgaa
    18481 gtgggccgac ctgtccagcc tgcgcgtcta cccgaccgat gccgcgcgcc aagcctcgct
    18541 ggaccccggc accgacgccc aggccggcga ggcgtggacc gaggtgctga ccctttcgcc
    18601 cgacgccgac atacctggca tgcaggccca gttcctatgc cactggcatt acgccgaact
    18661 cgtcgaaccc ggcaaggtca gctggaacct cgaaccgtgg cgaaatgagg tctccgaaga
    18721 gcagatggtc gcggcgggct gcaatccggg cggcacagaa gaaccgtttt agtgcggcgc
    18781 tacagccgtg ccggggtcgc ggcgctcgtc gaccacacac tgctcaagcc agaggccact
    18841 gaggccgaca tcgtcgcgct gttgcacgaa gccgacgaac tcgacgtctt cgcggtctgc
    18901 gtatcgccga cgatggtggc gacggccaag tcgttcaaaa cgggcgacta tcacatcgcc
    18961 tcggtcgtgg gattcccgtc aggcaaacat gtttcggcga tcaaggccga ggaggcccga
    19021 ctggctgtcg cggccggggc cgacgagatc gacatggtca tcgacgtcgg atcggcgttg
    19081 gaaggcgact tcgacgcggt gaaggccgat atctcagcgg ttttcgatgc gataccaagt
    19141 cacgccatct tgaaggtgat cgtggaatcc gccgccctgc ttgccttggc cgacgaaggg
    19201 cgactggccg acgtgtgccg catcgccgag gacgcaggcg ccgatttcgt caagacctcc
    19261 accggatttc accccagcgg gggcgcctcg gtacgcgctg tcgaggtgat ggcgcgcacg
    19321 gtcggagaac ggctgggcgt caaggccagc ggtggcatcc gcaccgcgga cgatgccgtc
    19381 gcgatgctca acgcgggcgc cacccggctc ggactatccg gcacccgcgc ggtgctcgac
    19441 gggctggatt agaggttcgc gaagcagggg tcttcgttct gcttcgggat cgttgcgttc
    19501 tgcgtcgaaa attggctcac cacaccggta tccgtgacct gcaccgagtc cgcatggatg
    19561 ccgagcgggt agttcttggt cagctgcgcg ctgaatgcgt ccagcgcggg ctggacggtc
    19621 tcccgcggaa gggcgaagcc gaggccggtc aattccgtca cctgcagcga gatcccgttg
    19681 tcgacgaccg ccggcttggc agtgatgttg ccgagcgcgc cctgcaactc gatggtccct
    19741 gcggacggat tggtcgtcac accggtgacg aacgagccga cgagcgggat cgcattctgg
    19801 atggtctcct tgatgccctg cgacgaccac gtgacgttcg cgaccaacga tccgatcgag
    19861 ccaccggaag tcgcggtgtc ctcgagccgg acgtccttga tgaccacgtt gaccttcatg
    19921 cccttggcct gacggatctg gttgccggcg gtctcgatgt ggatgttcgt gtagtggccc
    19981 gacacgtgct gcatcaggaa cggtggcaga gcaccgaacg aaaccgacgc gctgtcctgt
    20041 gttacgcatt cggttgcctt ggccactacc gagtccgccc ggctgcgcgc atacagctcg
    20101 cctgccagca ggccggccgc caccagagcg atgacgatga ccacgacgag gacgatggac
    20161 agcgggtcgc ggaagaggtt cttgaccttc cccggcagcg acgagtcctt ctgcggaggt
    20221 gcggcctgcg gaggtgcggc cgacggtggt gccggctggc ccggcggtgg gccttgcggg
    20281 aactgtggcg gcggtgcgac gggcgggccc tgattggccg ggcgagccca cggatcagtc
    20341 acctcagcga ttctgcctta tcgacgcgcg tgaactctga gcggttatgc aacaccgcga
    20401 tcgtctcgcg ggccttctgc gccgcatcga ggtccagatc ggacaccaac aactgcggat
    20461 ccgccccggc cgatgccaac acctctccga ccgcggacgc gaccagactg ccgccgacgc
    20521 caaccggccc ggcggaggct agttcgtctc cgggataggc ctggtccacg gcggcgacga
    20581 tgcccgtcgc gtcgatggcg cgggcccggg ccaacagcgt ccactgctcg agcttgccgg
    20641 gtccggtgcc ccatgacgcg tgcacggtga tgagttgcgc tccgcgctgg gccaattcga
    20701 cgtagagctc ggggaagcgg acgtcgtagc acagtgtgat cccgacgttc acgccgccga
    20761 ccgtgatcat cgccggttcg tgcccgggtg cgacggtgtc ggactccgcg aagccgaacg
    20821 cgtcgtagag gtgaatcttg tcgtagtgcg tgtcgatgcc gggaccggtg gcgatcaggg
    20881 tgttggtcac cctgccatcg tcggcaggaa cgaacatgcc ggcgacgacg acgatgcccg
    20941 cgcgctcggc aatcgcgcgg accgccgacg cccacgaccc gtccagcggc tctgcgatcg
    21001 gggccagcgg tacaccgaac cggcacatcg tcgcctccgg gaacagcacc agcgaggcgc
    21061 ctgcgtcggc ggcgcgtcgc gtgtagtcct cgaccaggcc gaggttcgcc gtgggttctg
    21121 caccgctctg gatctgcgcg agggcaatcc gcatgcggtc agcctagtgg cgcgaccatc
    21181 gaccagggca cggtaagcac gccgtcgcgc atccgccggc gcggcggctc gaccgggacg
    21241 ccctcgtcgc gcagcaagtc gagcatggcc cgccagcgca cccgcggccc gaacacgccg
    21301 tggcccgcga cgctggccca cgcgcggtcc gcggacatca gcagtgtgtg gacgggctgc
    21361 ccggcgacga tgtggtggat cagcaccttg ggcaaccgct cggccagatc cgacggtcgc
    21421 tcgatagcga acggatcgca tgccagcgtc aggctgatcg gcccgtcgac gtccagcagc
    21481 acccagcagc agcggcggcc caactcatcg caggtgccgt cgacgatcag tccgcccggc
    21541 gccagcgccg cctgcatcac cgaccacgcg gctggcacgt cttcgaccgg gtattgacgc
    21601 aacacgttga acgcgcgcac caaaaccggt ctgcgcccgg ctaattcgaa gccgccaagc
    21661 gcgaattcca cgccgtcgcg cggcggcacg atgcgttcgg ggtgaatctc cagcccgacg
    21721 acacgaacgt cggcccgtac cacccgcagc cgcgccgcca gctccagcgt cgtcaccggc
    21781 agcgcgccgt agccgaggtc gaccaccagc ggatcggccg ccgcctgcag cgcggtgcgc
    21841 acccgtcgcg agtgcaccag ccagcggtcg ctgcgacgca accggttatg gcccgtggtg
    21901 ccgcgggtga tggcgccgac gggcctagtg gtgcttggaa atccagtcag cggtgaacgc
    21961 ctcctcggcg tcgaagagct gtttgatgtg gtgcaggatc agatcttcca gcacgccgcc
    22021 gaccaagggg atgtgcacct tacagatgct ggccagccgc aacttgctgc cggcgtcggt
    22081 ctcggtcagg aagtactggc cgccaaagct gccggggccg tgcggaattg aggccgagta
    22141 ggtgcctttg gcccggttct tgtcgtggtc gtaggcgtcg aaatgctgct tgcgggtgat
    22201 gatcatgtcg accggcacga cggtccgcgc gatcggcggc agatacatcc tcggcaggtt
    22261 ctgcacgaag acgatgtcgg tgccacgctc ggtcgacgtg aaggacctga tctgcgactg
    22321 cggcgtcagg aaccggtacg agtccatcag ggtttgccag tactcccggt tcgtgaagtc
    22381 ctggtagatc ttctcggtcg gtgcgtcgaa ctcgatggtg taatccatgc gacgcgacat
    22441 ggaacataat cttacttacg ggttacgcgt tttcggtgat ccacaccgtc gtaaagcgct
    22501 gttctgcaat tagtaggtcg acgagctggc cgccgatgaa gttctcgatc tttccgccga
    22561 ccagcggcac gttgacctgg acggtggcgt tgaactccaa ccgcgagccg ccgcccgatt
    22621 ttgctgggac gagcacggcg ctgccggtca gtgtcgcggg cgcgtcgatg atcgcgaggt
    22681 cgagggtcgc ggtggactgc ccgtcgcgga tcgggctcca tttctcctcg cgaacaagga
    22741 ccaggtcgcc tcggtggaac tgggtcacca cggcaggtag ccggtcggcg cgcagccgct
    22801 gcgtggtctt tatgtcgatg ccgtcgcctt cgacggtcag aaaatccagc gcgtagtcgt
    22861 cggcgccgga atcggccaac cgcgccaacc aatactgttc gtcgccgaat gccaggtgaa
    22921 cctgttcgac gctgcccccg tattcggtcg ccatgtcgaa tgaacgcggc atagctggcc
    22981 aggctaccgt tacgccccgt gaccggtctg gatctggggg gcgcggccgt cgccgacgac
    23041 gtgccgttgg cgccgctgac cacgctgcgc atcggacccg ttgcacgccg gttgatcaca
    23101 tgcacgagca ctgagcagat cctcgccgcg ctgcgggcgc tcgacgatgt gctggtgctt
    23161 gccggtggat cgaatgtcgt actggccgat gacctcgaag acctcactgt cgtgctgctc
    23221 gcgaacaccg aaatcaccgt cgagggcaac atcgtgcgcg ccgaagccgg cgcggtgtgg
    23281 gacgacgtcg tggtcacctc gctggcccac gggctcggcg ggttggagtg cctgtcaggc
    23341 atccccggat cagtgggcgc gacgcccgtg cagaacgtcg gagcctacgg cgccgaggtc
    23401 gccgacacca tccgccgcgt gcgactgctc gaccgccaca ccggcgaaga ccgctgggtg
    23461 tcgccggacg cactccaatt cggttatcgc acaagcattc tcagacactc ggcggccgcg
    23521 atcgtgctcg aggtggagtt cgcgttggac gccgacgggc gcagcgcgcc gctgcgctac
    23581 ggcgaactgg ccggcgcgct ggacgccgag cccgggtccc gcgccgaccc cgcacgcgtg
    23641 cgggaggccg tgctgtcgct gcggacccgc aaaggcatgg tgctcgacga tgccgaccac
    23701 gacacctgga gcgtcgggtc gttcttcacc aaccccgtcg tcagcaccga tgaattcgac
    23761 cgggtagcgc gccgcaccga cgggccggtg ccgaactacc cggcacctaa cggcgtgaaa
    23821 ctggccgcgg gctggctggt ggagcgggcc ggcttcggca agggctaccc cggcgatggc
    23881 gcgcgggcac gactgtccac caagcatgcg ctggcgctga cgaaccgcgg cgatgccacg
    23941 accgaggacg taatcgcgct ggccaggacg gtacgcgacg gtgttaggac gacattcggg
    24001 atcgatctca cacccgagcc gattctgatt ggggccgctc tctaggtacc ctgggtccac
    24061 gtgagcccag ccgatccctt gccgtcgatg aaccggcggc gcattcttac cgccctggcg
    24121 gtaggagtcg ttgcaccggg cgcactcgcc gcctgcggta gcaggatcag caaaccgtcc
    24181 gcggacgcac cggccgcacc cgccgggccg tcgatcagct accagcccgc caacgccacc
    24241 accgacgtgg cacccgtcac gccggtgcgc gtggaggtca aggacggctg gttccagcgc
    24301 gtcgcgctga ccaatcccga cggcaaggtc gttgcggggg cgctcaaccg cgatcgcacc
    24361 gtcttcaaca ccaccgaacc gctcggctac ggcgtttcgt acacgtgggc cgggtcggtg
    24421 gtcggccatg acggtaaggc cgtcccggtc gagggcaact tcaccaccat caacccgagc
    24481 actcaggtca acggtcaatt ccagctcgcc gacggtcaga cggttggggt cgccgcgccg
    24541 gtcatcattc agttcgacgc gtcgatcacc gacaaggcat ccgtcgagaa ggcgctcaag
    24601 gtcacaacgg agccgccggt cgagggcagc tgggcgtggc tgcccgacga ggtcggcgga
    24661 tcgcgggcgc actaccgaac caaggagtac tacccgccgg gcaccaaggt cagcgtcgac
    24721 gcgaagctct acggcgtgcc attcggcgac ggcgcctacg gcgcgcagga catctcgctg
    24781 aacttcgcga tcggccgccg ccaggtggtc aaggccgagg cctcgaccca ccgcatccag
    24841 gtgctcgacg aacagggcgc ggtgatcatg gacttcccgt gcagctacgg cgaggccgac
    24901 ctcccgcgca atatcacccg cagcggaatc cacgtcgtca ccgagaagta cgaggacttc
    24961 tacatgacca acccggccgg cggctacagc aatgcgcacg agcggttcgc ggtgcggatc
    25021 tccaacaacg gcgagttcat ccacgccaac ccggccagct ccggtgcaca gggcaacacc
    25081 aacgtcacca atggctgcat caacctgtcg acgagtgacg cccagcagta cttcaacagc
    25141 gcgatctacg gcgacccggt cgaggtcacc ggcacctcga tcgagctgtc ctactccgac
    25201 ggggacctct gggactgggc cgtcagctgg gacga
//
LOCUS       DASRJA010000719        10702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3966134,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000719 DASRJA010000000
VERSION     DASRJA010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10702
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3966134"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFC01_32480"
     CDS             <1..335
                     /locus_tag="VFC01_32480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN84374.1"
                     /translation="GGTLGVAIVGSVFASIYSSRLSDSAVLAALPAQARGTMERSMAA
                     AQQVIGQLPQGVIPDVRAAVNTAFLDGLQIGSLVSAGIALGAAIVVAWLLPARARQTT
                     LQPEAVSL"
     gene            332..1189
                     /locus_tag="VFC01_32485"
     CDS             332..1189
                     /locus_tag="VFC01_32485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN84375.1"
                     /translation="MSTYTDTWTDGHGPIQYFTRSDGSRLRYFTAGSGPVLVLMHTIR
                     TQLDYFHRVIPQLWDSFTVYALDLPGMGWSDIVPGARYEEPNLRAAVVDFVTGLDLHD
                     VILAGESLGGALALQASVDLKDRVSRVVAFNPYDYPSGLERGNLFARFIISSVRMPWL
                     GPLFAAMENRLIMKRVMRGGVVDNATLPDDFIVELRKSGRRKGYTHVARAVYRSLRGF
                     VKARDRYRDVAVPVTLVYSDQDWSRPAEREHVANLLTEVERITLPDTGHFSALERPNE
                     VARILRRSN"
     gene            1226..2731
                     /locus_tag="VFC01_32490"
     CDS             1226..2731
                     /locus_tag="VFC01_32490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZN84376.1"
                     /translation="MDADYIVVGTGSAGSVVANRLSADPSASVVVLEAGPRDKDKFIH
                     IPAGFAKLFRSDMDWDYLTEPQKELDGREIYWPRGKVLGGSSSMNAMMWVRGFPADYD
                     EWAEHAGEQWSYATVEKYFNRIESGPLVISPQRSPRTSTAAWLQAARECGHEGFVETR
                     VTQRRGARWSTADAYLKPALQRKNLTLHTEATVTRILFDGTRAAGVEFEQLGARRVVK
                     ARREVVLCAGAINSPQLLMLSGIDARDQLAAHGIDVVHELPGVGANLLDHLVVPIGFD
                     VPNDTLFTAEKPVQLVNYLVRRRGMLTSNVGEAYGFVKSRPELELPDLELVFAPAPYF
                     EEGIGDPYDRHAVVMGPILLKPHSTGTITLRSADPKDKPIIDPAYLTDAAGADRSALM
                     AGLRMCADIARSAALKGIIGEVARPLGATDLDDETLERALNSLSHTLYHPAGTCRMGA
                     DDGSVVDTELRVRGVDGLRVADASVMPTIIRGHTHAPSVLIGERAADLIRG"
     gene            complement(2728..4182)
                     /locus_tag="VFC01_32495"
     CDS             complement(2728..4182)
                     /locus_tag="VFC01_32495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HZN84377.1"
                     /translation="MLVLNGPASIADVVALADRRDRVSVSPEVLKAVAHAHEVAAELG
                     TRVAIYGRTTGVGANRATSVSPTDTEYGMRLLRSHAVDAGDPLDDRTVRAMLAVRLVQ
                     LCVPGAGLDPRILAGLEAMLNDDALPELRQYASIGTGDLAPLAGTALTLIGERPASKP
                     LTPMEPWGADSALPFMSSSALTVGRGCLALDELSLLERASSVIYMLSFLALDGNPEAL
                     SSVAAQASAAPYVSTIADRLRSLLATKGGPNRRPARIQDPYGLRVYPVAQATVLASSH
                     SLEEQLERTLNSAQENPLFDIDDDAVVHHGAFYQASLSLELDGTTLALALAAPITHSR
                     IRMLNDPDTNGGNPFLAADAAGSSGLMMVEYVAAGAIAEIRNAAQPASAGTLVLSRGA
                     EEDATFASQGAQQLERSIAAYRVLLCCELVGAVRLLRQRGLDDQFSGAVGDALALADG
                     LPDNTEDRDLRGDIASAESLLDDMGRLVPPDSRP"
     gene            4345..5028
                     /locus_tag="VFC01_32500"
     CDS             4345..5028
                     /locus_tag="VFC01_32500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN84378.1"
                     /translation="MAEPSTRTVERALALLATICDRGGANLADSARECDLAPSTALRL
                     LRTLENTGFVAKEEAGGTYRPGSRLIQLGAQALSNESLIDFAKPEMEKLVDDTGESAY
                     LSVEGHAGTALYISIVEGTHSVRHASWVGRTVPVDISAAGQALRGRVPDEGYVIVERG
                     VEADVTAIASPIYSEARIVAALSLVVPSYRLTASQAAEYGRTLAKTAAELSAGLSRRP
                     KSPLPEGQS"
     gene            5025..6128
                     /locus_tag="VFC01_32505"
     CDS             5025..6128
                     /locus_tag="VFC01_32505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN84379.1"
                     /translation="MITFDNVSKVYPDGTVAVDKLTITAPNGKITTLVGPSGCGKTTS
                     MRMINRLIEPTSGSITLDEVDTQTLDRVALRRRIGYVIQNAGLFPHRTVVDNVAALPR
                     LLGGGKKETRSAAMELLERVGLDAKFAKRYPWQLSGGQQQRVGVARALATDPPFLLMD
                     EPFSAVDPIVRSQLQDEFLRLQADISKTIVMVTHDIDEALKLGDQVVVLRQGGTLAQA
                     ATPAELLASPNDAFVADFVGSARGYRALGFHTSDDLLKLNDEPTITVGQPVSTITSQG
                     RWVLVVDEKRAPLGWVDTEGVRGDSVQDSDVNLCATSATIGDPLRDLLNSALSSPSGR
                     CVVTDESGVLLGTASDHDVIDAIRRAKEARTAA"
     gene            6125..6769
                     /locus_tag="VFC01_32510"
     CDS             6125..6769
                     /locus_tag="VFC01_32510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN84380.1"
                     /translation="MNWISSNIDRIVSLTLSHVWLTLAPTVIGLLIALPLGWWAHRFK
                     RGQAAIVGTAGLLYTIPSLALFIILPTILGTKILDPINIVVALTLYTLALLVRVVADG
                     LDSVPHDTVAAAEAMGYRAWQRLVLVELPVAVPVIAAGLRVAVVSNVSIVTMAALLGI
                     PQLGSLFTQGFQLRLYVPLLTGIVLCVVLAVVFDGLIIWLNRLLTPWRQRTVTS"
     gene            6742..7494
                     /locus_tag="VFC01_32515"
     CDS             6742..7494
                     /locus_tag="VFC01_32515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN84381.1"
                     /translation="MAAKDGDLVNIMSWFTDPSNWTGSGGIPAQVGYHLFYSAIALLA
                     ALVIAVPLGILIGYTGRGEALVAGSANALRALPSLGLLVLLFLIISPVVSGKLVYVLP
                     TIIVLVLLAVPPILTGTYAGIQTADPDAVGAARGMGFTKRQILLRVQLPCALPLMVSG
                     VRSSTLQIVSTATIAAYLGLQGLGRFILDGRANASFAEMAGGAILVAILALVLEFTFA
                     WLGRVIVSPGLRRTVSKSSSPISTTVLEPATT"
     gene            7505..8428
                     /locus_tag="VFC01_32520"
     CDS             7505..8428
                     /locus_tag="VFC01_32520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN84382.1"
                     /translation="MKKRTLLSALAVTSVLALSACGGGGGDPLSGGGNNTGSSGSEVI
                     VGSADFTESQLIASIYSQALQSAGVPTKEQFNIGSREVYIQALKDGSIDLVPEYTGAL
                     LSYLDPKSTAATSEAVTAELKGKLPSGTSMLTPSPAEDKDVVAVTQATADKYKLKTIS
                     DLKPVAGELVLGGPPEWKTRTQGVVGLRDVYGLNFKDFVSLDAGGTLTMTALTNGQIQ
                     AGDLFSTDPGLHANHLVALEDDKSLFAAENVVPLIKSGKQNDTVTKTLDAVSAKLTTD
                     DLIDMNAEAAKGTNLSDIAKKWLADAGIAKS"
     gene            complement(8449..9435)
                     /locus_tag="VFC01_32525"
                     /pseudo
     CDS             complement(8449..9435)
                     /locus_tag="VFC01_32525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856384.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            9528..>10702
                     /locus_tag="VFC01_32530"
     CDS             9528..>10702
                     /locus_tag="VFC01_32530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZN84383.1"
                     /translation="MTMNAKRRRAHDKLAALPGVRAVRRPTRPDGDQEFDLFYVRTGR
                     KSAHPLVVIPGGPGAASIALYRGLRRRAAVEGIDVLMIEHRGVGMSRHDDRGADLPPE
                     ALTINQVVDDVAAVLDDAQVDKAVVYGTSYGTYVAAGVGVRHPDRVYGMILDSPVLSA
                     HDIDAVREETRRLLWDGDAAETADLAPKVRQLVDQEMLTPEAAQLAAAVYGFAGPAVL
                     RRQLDLLLAGRRGLWSVLARGARFLFERKAPYRHEPDLVGRIGYRELNYAAVPDGKPL
                     DPAVAYHEISADTAEFETEPFNLSDEMPRFTWPTVVISGGRDLITPPAVARSIASLIP
                     GAVLVNLPTAGHSVLDLRERAALEIIKATYDGTSAGLPARAKKLDSTPGGLGVRALV"
BASE COUNT         1850 a         3558 c         3452 g         1842 t
ORIGIN      
        1 tgggcggcac gcttggtgtc gcgattgtcg gcagcgtgtt cgcgtccatc tactcgagcc
       61 ggctgtccga cagcgcggtg ctggcagcgc tgcctgccca ggcccgcggg acgatggaac
      121 gctcgatggc cgcagcacaa caggtcattg gtcaactacc gcagggtgtg attcccgacg
      181 ttcgcgccgc cgtcaacacc gcgttcctcg acggcctgca gatcggatcc ctggtcagcg
      241 ccggtatcgc gctgggagcc gcgatcgtcg tcgcctggct gctgcccgca cgggcccggc
      301 agacaacgct ccagcccgag gcggtgtcgt tatgagcacc tacaccgaca cctggaccga
      361 cggccacggg cccatccagt acttcacccg atccgacggc tcccgcctgc ggtacttcac
      421 cgcaggcagc ggcccggtgc tggtcctgat gcacacgatc cgtacgcagt tggactactt
      481 ccatcgcgtc atcccgcagc tgtgggactc gttcaccgtc tacgcactgg acctgcccgg
      541 catgggctgg tcggacatcg tgccaggggc gcgctacgaa gagcccaacc tgcgtgctgc
      601 ggtggtcgac ttcgtcaccg gcctcgacct gcacgacgtc atcctggccg gcgaatcgct
      661 cggcggcgcg ctcgcgctgc aggcgtctgt cgacctcaag gacagggtca gccgggtcgt
      721 tgcgttcaac ccctacgact atccaagcgg cctcgaaagg ggcaacttgt tcgctcggtt
      781 catcatcagc agtgttcgca tgccgtggct gggtccgctg ttcgccgcca tggagaaccg
      841 gctgatcatg aagcgtgtga tgcgcggggg tgtggtcgac aatgcgacgc tgccggatga
      901 cttcatcgtc gagctccgaa agagcggacg ccgcaaggga tatacccacg tcgcgcgagc
      961 cgtctatcgc agcctcaggg gattcgtcaa agcccgcgac cgctaccgcg acgtggcggt
     1021 gccggtgacg ttggtctaca gcgaccagga ctggtcacgg ccggcggagc gcgaacacgt
     1081 cgcgaacctg ctgacggagg tcgagcggat caccctgccc gacaccggcc acttctcggc
     1141 cctggaacgg ccgaacgagg tggccaggat tctgcggcga tcgaattgac cctgcgacac
     1201 ctgccggact cttagactcc cgcaggtgga cgccgactac atcgtcgtgg gaaccggctc
     1261 ggccggatcg gtcgtggcca accggctcag cgcggacccc tccgcgtcgg tggttgtgct
     1321 ggaagcaggg ccgcgcgaca aggacaagtt catccatatc cccgccggct tcgcgaagct
     1381 gtttcgcagc gacatggact gggactacct gaccgagccg cagaaggaac tcgacggccg
     1441 cgaaatctat tggccgcgag ggaaagtgct cggcggatcg tcgtcgatga acgcgatgat
     1501 gtgggtccgc ggcttccccg ccgactacga cgagtgggcc gagcacgcag gcgagcagtg
     1561 gagctacgcc actgtcgaga agtacttcaa ccggatcgaa agcggtccgc tggtcatctc
     1621 gccgcagcgc agcccccgca catcgacggc cgcctggctg caggccgcgc gcgagtgcgg
     1681 tcacgagggt ttcgtcgaaa cccgcgtcac gcaacgcagg ggagcgcgct ggagcaccgc
     1741 cgacgcgtac ctcaaacctg cactgcagcg aaagaacctg accctgcaca ccgaagccac
     1801 cgtcacgcga atactgttcg acgggacacg cgcggcgggc gtcgaattcg aacagctcgg
     1861 cgcgcgccgt gtcgtcaagg cccggcgcga ggtggtgttg tgcgccgggg cgatcaacag
     1921 cccgcaacta ctcatgttgt cggggatcga tgctcgcgat cagctcgccg ctcatggcat
     1981 cgacgtagtg cacgaactgc ccggggtcgg cgccaatctg ctggaccacc tcgtcgttcc
     2041 gattgggttc gacgtgccga acgacacctt gttcacagct gagaagccgg tgcagttggt
     2101 caattacctg gtgcgacggc ggggcatgct gacctcgaat gtgggggagg cgtacggctt
     2161 cgtcaagagc cgcccagagc tcgaactgcc cgatctggag ctggttttcg cgcctgcgcc
     2221 gtacttcgag gaaggcatcg gcgatcctta cgaccgccac gccgttgtca tggggccgat
     2281 cctgctcaag ccgcacagca cgggcacgat caccctgcgg tcggccgacc caaaggacaa
     2341 gccgatcatc gacccggcct acctcaccga cgccgctggc gctgaccgct ctgcgctaat
     2401 ggccgggctc cggatgtgtg ccgacatcgc cagatcggcg gcactgaagg gcatcatcgg
     2461 cgaggtggcg cggccgctcg gtgcgacgga cctcgacgac gaaacactag agcgggcgct
     2521 gaactcgttg tcgcacacgc tctatcaccc ggccggcaca tgccggatgg gcgccgacga
     2581 cggtagcgtg gtcgacacgg agctgcgggt gcgcggtgtg gacgggctgc gcgtcgccga
     2641 tgcgtcggtg atgcccacga tcatccgcgg ccacacccat gcgcccagcg tgctgatcgg
     2701 tgagagggcg gccgatctga tccgcggcta aggccggctg tctggcggaa cgagtcgacc
     2761 catgtcgtcc agcagtgatt ccgccgaagc gatatccccc cgcagatcgc ggtcttcggt
     2821 gttgtccggc agaccgtcgg cgagcgccag cgcgtcgccc acggcgccgc tgaactgatc
     2881 atcgagcccg cgctggcgca aaagtcgcac cgcgccaacg agttcgcagc acagcaacac
     2941 ccggtacgcg gcgattgatc gctcgagttg ctgcgcgccc tgggaggcga aagtggcgtc
     3001 ctcttcagca ccccgcgaca gcactaacgt gccggcggat gcaggctgag cggcgttgcg
     3061 gatctcggcg attgctcccg ctgccacgta ttcgaccatc atcagcccgg atgacccggc
     3121 cgcatcggcg gccaagaagg ggttgcctcc gttggtatcc gggtcgttga gcattctgat
     3181 ccgagaatgc gtgatcggtg cggcaagtgc gagcgccagc gtggtgccgt ccaattccaa
     3241 tgacaacgac gcttgataga aggcaccgtg gtgaaccact gcgtcgtcgt cgatgtcgaa
     3301 cagagggttt tcctgcgcgc tgttgagcgt cctctcgagt tgttcctcca gcgaatgcga
     3361 cgacgccagc acggtcgcct gcgctaccgg gtacacccgc aacccatacg gatcctggat
     3421 gcgagcgggc cggcggttcg gcccgccctt ggtcgccagc agggagcgca ggcgatctgc
     3481 aatggtcgag acgtacggcg ctgctgacgc ctgcgccgcg accgacgaaa gcgcctcggg
     3541 attgccgtca agcgcaagaa agctcagcat gtagatgacg ctcgacgctc tttcgaggag
     3601 tgaaagttcg tccagcgcaa gacaaccccg cccgaccgtc agtgcgctgc tgctcatgaa
     3661 cggtagggcg ctgtcggcgc cccacggctc catcggcgtc agtggcttgg acgcggggcg
     3721 ttctccgatc aacgtcaacg cggtgccggc aaggggagcg aggtcgcccg tcccgatgga
     3781 cgcgtattgc cgaagttcgg gcagggcgtc gtcgttgagc atcgcctcga gtccggccaa
     3841 gatcctggga tcgagtccgg cgccgggcac gcatagttga accaggcgaa ccgcgagcat
     3901 cgctcgcacc gttctgtcgt caaggggatc gccggcgtcg accgcgtggc tgcgaagaag
     3961 tcgcatcccg tattcggtat cggtcggcga tacggacgtg gcccgattcg cgccgacacc
     4021 cgtcgtccgt ccgtagatcg cgacccgggt gcccagctcc gctgccacct catgcgcgtg
     4081 ggcgacagcc ttcagaactt ccggcgaaac cgagacccga tcgcggcggt ccgcgagcgc
     4141 cacgacatcg gcgatactcg cgggtccgtt aaggacaagc atgaaattct cctgatcctc
     4201 tatcgctggc gagcgatacg tcctatcatc accgaaacca cacattgccg ctagatggaa
     4261 tttgaatttt actcaatgga aatccggaga gggctgatcg caacaaaccc ctcgtagatt
     4321 ccatcccggc gagaggaggt cacggtggcc gaaccgtcca cgcggaccgt ggaacgcgca
     4381 ctcgccctgc tcgcgaccat ctgcgacagg ggcggcgcga atctggccga cagcgcccgg
     4441 gagtgtgatc tcgcgccgag tacggcactt cggctcctcc gcacgctgga gaacacaggg
     4501 ttcgtcgcca aagaggaggc cggtggtacg taccgccccg gcagtcgcct gatccaactc
     4561 ggcgcgcaag cgctgtccaa tgagtcgctg atcgacttcg ccaagcctga gatggagaag
     4621 ctcgtcgacg atacgggaga gtccgcgtac ctgagtgtcg agggccacgc aggcacggcg
     4681 ctctacatca gcatcgtcga gggcacccat tcggtccggc acgcaagctg ggtcggtcgt
     4741 accgttccag tcgacatctc cgcggcggga caagcgctgc gcggccgtgt gcccgacgag
     4801 ggatacgtga tcgtcgaacg cggcgtcgag gccgacgtca ccgcgattgc ctctccgatc
     4861 tattccgagg cgcgcatcgt cgcagccctg agcctcgtcg tcccgagcta ccggctcacc
     4921 gcatcccaag cagccgaata cggccggacg ctcgccaaaa cggcggccga gctgtccgcc
     4981 ggattgagtc ggcgcccgaa atcacctctg ccagaaggac aatcatgatc acattcgaca
     5041 atgtctccaa ggtctatccc gacggcaccg tcgcggtcga caagctgacg atcaccgcgc
     5101 caaacggcaa gatcaccacc ctggtcggtc cgtcgggctg cggcaagacc acctcgatgc
     5161 gcatgatcaa ccggctgatc gaaccgacgt cgggcagcat cacactcgac gaagtcgaca
     5221 cccagaccct cgacagggtc gccctgcgcc gccgaatcgg ctacgtcatc cagaacgccg
     5281 gtctctttcc gcaccgcacc gtcgtcgaca atgtggccgc cctgccgcgg ttgctcggcg
     5341 gcggcaagaa ggaaacccgt tcggcggcaa tggaactgct cgagagagtc gggctcgacg
     5401 ccaagttcgc caagcgctac ccgtggcagc tctccggcgg tcaacagcag agagtcggag
     5461 tggcgcgggc gttggcgacg gatccgccgt tcctgctgat ggacgaaccc ttcagcgccg
     5521 tcgacccgat cgtgcggagc cagttgcagg acgagttcct acggctgcag gccgacatct
     5581 cgaagaccat cgtcatggtc acccacgaca tcgacgaagc gctcaaattg ggtgatcagg
     5641 tggtcgtgct tcggcagggt gggacgctgg ctcaagcggc gaccccggcc gaactgctgg
     5701 cctcgccgaa cgatgccttc gtcgcggact tcgtcggctc cgctcgcggg tatcgggcgc
     5761 tcgggttcca cacctccgac gacctcctca aactcaacga cgagccgact atcactgtgg
     5821 ggcagccagt ctcgacgatc acctcgcaag gccgctgggt cctcgtcgtc gatgagaagc
     5881 gggcgccgct cggctgggtc gacacggaag gcgtgcgcgg cgactccgta caggacagtg
     5941 acgtcaacct ctgtgccact tcggcgacta tcggcgaccc gctgcgtgac ctgttgaact
     6001 cggcgctgtc cagtccgagc ggacgctgtg tggtcaccga cgagtcgggt gtgctgctcg
     6061 gcaccgcatc cgatcacgac gtcatcgacg cgatccgccg cgcgaaggaa gcgaggacag
     6121 cggcatgaac tggatctcgt ccaatatcga ccgcatcgta tcgctgaccc tcagccacgt
     6181 ttggctcacc ctggcgccga cggtcatcgg gctcctcatc gcgctcccgc tcggctggtg
     6241 ggcacatcgg ttcaagcgtg gccaggccgc catcgtcgga accgctggac tgctctacac
     6301 catcccctca ctcgcgctgt tcatcatctt gccgaccatc ctcgggacga agattcttga
     6361 tccgatcaac atcgttgtcg cgctgacgct ttacaccttg gctctgctgg tgcgcgtcgt
     6421 tgcggacgga ttggactcgg tgccacacga caccgtggcc gccgccgagg ccatgggtta
     6481 ccgggcctgg cagcggcttg tgctcgtcga gctgcctgtc gccgttccgg tcatcgccgc
     6541 gggcttgcgc gtcgcggtcg tgtccaacgt cagcatcgtg accatggcgg cgctgctcgg
     6601 aatcccgcag ctcgggtcgc tgttcacgca gggctttcag ctgcggctct acgttcctct
     6661 tctcaccggc atcgtgttgt gcgtggtgct tgcggttgtt ttcgacggcc tgatcatctg
     6721 gctgaacaga ctcttgaccc cgtggcggca aaggacggtg acctcgtgaa catcatgtcg
     6781 tggttcactg atccatcgaa ttggactggc tcaggcggta tcccggccca ggttggctat
     6841 cacctcttct actcggcgat cgcgctgctg gcagccctgg tcatcgcggt gccgctgggc
     6901 attctcatcg gctacaccgg acgcggtgag gcgctggtgg cggggtcggc caacgcgttg
     6961 cgggcactcc cgtctttggg gctgctcgtg cttctcttcc tgatcatctc gccggtcgtg
     7021 tccggcaagc tcgtctacgt gctgcccacc atcattgtgc ttgtgctgct tgcggttccg
     7081 ccgatcctga cgggaacgta cgctggcatt cagaccgccg accccgacgc cgtgggcgcg
     7141 gcccgtggga tgggcttcac gaaacgccag atcctgttgc gggtgcagct gccgtgcgca
     7201 ttgccgctga tggtctcggg ggtgagaagc tccacacttc agatcgtttc gacggccacc
     7261 atcgccgcgt atctcggtct gcagggtcta ggccgcttca ttctcgacgg cagggcgaac
     7321 gcaagctttg ccgaaatggc aggcggagca atccttgtcg cgatcctcgc gctcgtcctc
     7381 gagttcacct tcgcgtggct gggccgcgtc atcgtctcgc ccggcctgcg ccgcaccgta
     7441 tcgaaatcca gctcaccgat atccacgacc gtcctcgagc cggccaccac ctaggagaat
     7501 caccatgaag aaacgcacac tgctgtcggc gctggccgtc acttccgttc tggcactgtc
     7561 cgcatgcggc ggtggcggcg gcgatccgct ctccggcgga ggaaacaaca ccggatcctc
     7621 gggttcggaa gtcattgtgg ggtctgcgga cttcaccgaa agccagttga tcgccagcat
     7681 ctactcgcag gcgctgcaat ccgcaggcgt tcctaccaag gagcagttca acatcggcag
     7741 ccgcgaggtc tacatccagg cgctcaagga cgggtcgatc gacttggttc ccgagtacac
     7801 cggtgcgctg ctgagttacc tcgaccccaa gtcgacggct gcgacgagcg aggcggtaac
     7861 ggccgaactc aaaggcaagt tgccgtccgg aacgtccatg ctgacgccgt ctccggcgga
     7921 ggacaaggat gtcgtggccg tgacacaggc caccgccgac aagtacaagt tgaagaccat
     7981 ctcggacttg aagccggtcg ccggcgaact cgtgctcggg gggccgccgg agtggaagac
     8041 gcgcacgcag ggcgtcgtcg gcctgcgcga cgtgtacggc ctcaatttca aggatttcgt
     8101 cagtctcgac gccggtggca cactgacgat gacggcgctg accaacggtc agatccaggc
     8161 cggagacctg ttcagcaccg atccaggtct gcacgcgaac catcttgtcg cgcttgagga
     8221 tgacaagagc ttgttcgccg cggagaacgt tgttccgctg atcaagtccg ggaagcaaaa
     8281 cgacaccgtc accaagacgc tggacgccgt ctcggcgaag ctcaccaccg acgacctgat
     8341 cgacatgaac gctgaggcgg ccaagggcac caacctgtcc gatatcgcga agaagtggct
     8401 cgccgacgcg ggcatcgcca agagctgacc tccgcgattt gccttcaact gggcgtcggc
     8461 ggccaggcgt gcgtcgggta tgccctcagt cgcagtttcg gtggccgctg cgatcgcctc
     8521 cgctgcgtcc agtactgctg ccttcgcgat atacgccgtc gcggccagtt caccgagttg
     8581 cgtctggtac agcggatcgt cggtcgggcg ctcggacagc gtatggctga agctgcgatc
     8641 ccgcgatcgc agcagcgcga caccgtcgtc caccacgctc gcgaggatgc ccgcgacgac
     8701 cgcatggatg aacagctgca gcgacgcgta ctgcaccgtc ggcaccgggt cggcgtcata
     8761 cggcgtgtcg gcccgcacct cgttgcccga caccgccacg ctggtgaaca ccgtggtgcc
     8821 ggtgcggcgc tgcccaaagc cgtcccagtc gtcgatgagg cggacgcctt cgcggtcggc
     8881 cggcaccacc accgtggcga ccgagtcgtg gtcagtggtg gtggcgacgg tcaggtagtc
     8941 ggagaacagc gtgccagtgc tgtagtactt ctcgccgctc agccgatagc cgccaggaga
     9001 actggggtcg ggcagcagcc gggtgttgaa caccaggctg ccgacggcca gcgagccctt
     9061 ttcgctgaac gcgttgccga agatctttcc ctccgcgacc ttgctcaacc actgctgcga
     9121 agctgggtca tgcgcggtgc gcagccgctc ctcgacgaac cagaagtgtg tccggaagat
     9181 atgggccaca atgggatcgg catgcgcgac gtcgatcgcc gcggagaata gttgcggcac
     9241 agtgaatccc gcgccgccca gatctgcggg cagtcgcagc gcgccgaaac ctgcgcgctt
     9301 gagtgcggca acttggtcaa atgggttctc gtaattgaga tctcggtcct ttgcgccgga
     9361 accgatgccg gccagcaggt cggcccattc atccgaaccg ggcaggatgc gagtcgcgtg
     9421 gtccacggag gtcactggcc tcgttttacc gacactacat cgcgatacgc actgcttttg
     9481 gatcatcccg aacctaacga cggtcgaccg gggcagaatg agtggtgatg acgatgaacg
     9541 ccaagcgtcg gcgggcccac gacaagctgg ccgcgttgcc aggcgtgcgt gcggtgcgac
     9601 ggcccacccg gccggacggc gaccaggagt tcgacctgtt ctacgtgcgc accggccgca
     9661 aatcggcgca tccgctggtc gtcatccccg gcggccccgg cgccgcctcc attgccctct
     9721 accgcggctt gcggcgacgc gcggccgtgg agggcatcga cgtgctcatg atcgaacatc
     9781 gcggcgtggg gatgtcccgc cacgacgaca ggggcgctga cctgccgcct gaggcgctga
     9841 caatcaacca ggtggtcgat gacgtggccg ctgtcctcga cgacgcccag gtggacaagg
     9901 ccgtggtcta cggcacctcg tacggcacct atgtcgccgc cggagtcggt gtgcggcacc
     9961 cggaccgggt gtacgggatg attctcgact cacccgtgtt gtccgcccac gacatcgacg
    10021 ccgtgcggga ggagacccgc cggctgctgt gggatggcga cgccgcagag accgctgacc
    10081 tggcgcccaa ggtgcgccag ctggtggacc aggagatgtt gaccccggag gccgcccagc
    10141 tggccgcggc ggtgtacggc ttcgcaggcc ccgcggtgct gcgccgacaa ctcgacctcc
    10201 tgctggccgg gcgccggggg ttgtggtccg tgctggcccg gggcgcccga ttcctattcg
    10261 aacgcaaggc gccgtaccgt cacgagccgg acctggtcgg caggatcggg taccgcgaac
    10321 tgaactacgc cgcggtcccc gacggaaagc cgctcgaccc cgccgtcgcg tatcacgaaa
    10381 tatcagcaga tacagcggaa ttcgagaccg aaccgttcaa cctcagtgat gagatgccca
    10441 ggttcacctg gcctacggtc gtgatttccg gtggccgcga tctgatcacc ccgcccgcgg
    10501 tcgcccgcag catcgcgtca ctgatcccgg gcgcggtact ggtcaatctc cctaccgccg
    10561 gacattccgt cctcgacctt cgcgagcgtg cggcgctgga gatcatcaag gccacctacg
    10621 acggaacgag cgcaggcctg cccgcgcgcg ccaagaagct ggactcgacg ccgggtggcc
    10681 tcggggtgcg ggcgctggtc tg
//
LOCUS       DASRJA010000720         4248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_952074,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000720 DASRJA010000000
VERSION     DASRJA010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952074"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VFC01_32535"
     CDS             complement(<1..1049)
                     /locus_tag="VFC01_32535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HZN84384.1"
                     /translation="MARDEGTRAVLVTESGPVKVETTRPRLRTGRRRIGDGLVEIPIR
                     EDIEPASAILTNPVLAESKRDCGSCGQPVGRTVAGRPGASEGVCPHCGTPFSFSPQLE
                     GGELVADQYEVQGCIAHGGVGWIYLAIDRNVSDRWVVLKGLLQPGGQQAQAIAVAERQ
                     FLAMVNHPGIVKIYNFVEHPGFDGRPIGYIVMEYIGGTTLQAILAKQRAETPKGQPKP
                     MMPVEQALGYLLDVMPALSYLHSLGLVYNDLKPDNIMLTEDNLELIDMGAVSGIGDYG
                     YIYGTKGFQAPEIVKTGPTVATDIYTLGRTLAKLTVDLPNDRYAESLPDPGEVPLFER
                     YESFYRLLVRATNPRP"
     gene            complement(1051..1992)
                     /locus_tag="VFC01_32540"
     CDS             complement(1051..1992)
                     /locus_tag="VFC01_32540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZN84385.1"
                     /translation="MRAIPTVLALAVVLLVGCSTVQPLVEVSPSLTTPPMPSGAEVAP
                     SVSGAAVESCDATASLRPSTEPGPTVEAIRRRGRVIVGIDQNNNPLSFRDPVTGELEG
                     FLVDISREIVRDLVGDPDKADFRLVTEPERIPAVQNQSVDILTKATTITCPRAEQIAF
                     STVYFEGSQRLLVPRGSPVRGPADLAGKRVCSGLATTSIATVARVAPAATLLGVPNMD
                     DCLVALQQGQADAASTDDVLLAGMVVQDPNLEIVGPSLEAEPYGIGINKSQDDLVRAV
                     NASLERIRRDGTWAALFRKWLTALGPAPSPPEPKYRD"
     gene            complement(1992..3299)
                     /locus_tag="VFC01_32545"
     CDS             complement(1992..3299)
                     /locus_tag="VFC01_32545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019972873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84386.1"
                     /translation="MHSDELAHQRTRTLDESLLVDPEESDRPVVDFLRATPGRIALVA
                     VVLVVALLAVGATASKTVFDRQGQLETLRSHTEPLADAAQRIYGALSYANTTAATAFL
                     SGGVEPRDVRDRYDAAIGQASAGMVTASNGVSPNDIHSLTLLTDISNQLAVYTGIVAT
                     ARAKNRAGSPIGVAYLSESSALMQQGILPAAERLYASQSDAVIASGRSVGPPRVVIAA
                     AVVVLVLLVLSQAALARRSHRRLNPGLVLASLLMAVLVVWLTVAGLVSTHAATSARTH
                     GGGPLDTAVSARILAQQARAEEVLGLLKRGSDPLSDIRFDERTAQIGWLLDEHRVDGA
                     ADALHGWMRSHDEIRRKLAGGDYGGAVAIARDDAPQHSTVQFTRFDTALRDEIARLRD
                     RQRDGIAQAYTALNGLPVGAAVISVLAALAVAAGIAPRLSEYH"
     gene            complement(3476..4159)
                     /locus_tag="VFC01_32550"
     CDS             complement(3476..4159)
                     /locus_tag="VFC01_32550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84387.1"
                     /translation="MAGIICVPKPPKSVPLQQKCLNHDVRCSETAPLIGPVAAIAATL
                     CDAWRRSGAWSGEVGRKRPCHGEPIPIYLRFQLHQATHLEANHLGLLVAYDQQRGKPA
                     EERHVTHERKRLVLGPRSEPLRYRYHRVIRPKTRSRLDARLHADLPDQQVRSLLCAQL
                     SAVQAAAELDAGGPGSFSDTLHRSSTGIREWSLRILGFGRRLTVLHQVKIHIGHLRWF
                     HHHPADAWS"
BASE COUNT          719 a         1410 c         1411 g          708 t
ORIGIN      
        1 ggacgaggat tcgtcgcacg caccagtagc cggtagaacg actcgtagcg ctcgaacagc
       61 ggcacctcgc cggggtcggg caatgactcg gcgtagcggt cgttgggcag gtcgacggtc
      121 agcttggcca gcgtacggcc caaggtgtag atgtcggtcg ccaccgtggg gccggtcttg
      181 acgatctcgg gtgcctggaa tcccttggtg ccgtagatgt atccgtagtc accgatgccc
      241 gacacggcgc ccatgtcgat cagctcgagg ttgtcctcgg tgagcatgat gttgtccggc
      301 ttgaggtcgt tgtagaccag gcccagcgag tgcagatagg acagcgccgg catgacgtcc
      361 agcagatagc cgagcgcctg ctccaccggc atcatcggct tgggttggcc cttcggcgtt
      421 tccgctcgct gcttggccag gatcgcctgc agcgtggtgc cgccgatgta ctccatcacg
      481 atgtagccga tcggacgacc gtcgaaaccc gggtgttcga cgaagttgta gatcttgacg
      541 atgccgggat ggttcaccat ggcgaggaat tggcgttcgg cgacggcgat cgcctgcgcc
      601 tgctgtccgc ccggctggag tagacccttg agtaccaccc accggtcgct cacgttgcgg
      661 tcgatcgcca gatagatcca gccgacgccg ccgtgggcga tacagccctg aacctcgtac
      721 tggtcggcca ccaattcgcc gccctccagt tgtggcgaga acgagaacgg cgtgccgcaa
      781 tgcgggcaca caccttcgct ggccccaggc cggccggcaa cagtccgccc gaccggctgc
      841 ccgcagctgc cgcagtcccg tttcgattcg gccagaaccg gattggtgag aatcgcactg
      901 gcaggctcga tgtcctcacg aatcggaatc tcgaccaggc cgtcgccgat gcgccgccgc
      961 ccggtgcgca gccgagggcg ggtggtctcg accttgaccg gccccgattc ggtcaccagg
     1021 acggcgcgcg ttccctcgtc tcgagccatg tcaatcccga tacttcggtt ccgggggact
     1081 cggcgccggg ccgagcgcag tgagccactt ccggaacaac gccgcccacg tcccgtcgcg
     1141 tcgtatccgc tccaggctgg cattgaccgc tcgcaccaga tcgtcctggg acttgttgat
     1201 tccgataccg tacggctcgg cctccaggct cggcccgacg atctccaggt tgggatcctg
     1261 tacgaccatg ccggccagaa gcacatcgtc ggtgctggcc gcgtccgcct gaccctgctg
     1321 aagcgcaacc aggcagtcgt ccatattcgg aaccccgaga agggtggccg ccggcgcgac
     1381 ccgggccacg gtggcaatgg atgtcgtcgc cagcccggag cagacccgct tgccggccag
     1441 gtcggccggc ccccgcaccg gcgaaccccg tggcaccagc aaccgttggc ttccttcgaa
     1501 gtacaccgtg gagaaggcga tctgctcggc acggggacag gtgatcgtcg tcgccttcgt
     1561 gaggatgtcg acgctctggt tttgcaccgc gggaatacgc tccggctccg tcaccaggcg
     1621 gaagtccgct ttgtcgggat cgccgaccag gtcccggacg atctcgcggg agatgtcgac
     1681 gaggaagccc tccagttcgc cggtcaccgg atcgcgaaaa ctgagcggat tgttgttctg
     1741 gtcaatgccg acaatcactc ggccgcggcg ccggatcgcc tccaccgtgg gcccgggctc
     1801 ggtggacggg cgcaaactcg cggtggcatc acaactctcg accgccgcgc cggacacgga
     1861 cggcgccacc tcggccccgc tgggcatcgg cggcgtggtg agagatggcg acacctcaac
     1921 gagtggctgc accgtggaac accccacgag caggacgacg gcaagcgcga gaaccgttgg
     1981 gatcgcgcgc atcagtggta ctcactcagt cgtggtgcga taccggcagc caccgccagc
     2041 gccgccagca cactgatgac cgctgcaccg accgggagcc cattcagcgc ggtgtaggcc
     2101 tgtgcgatcc cgtcgcgctg tcgatcgcgc agtcgcgcga tctcgtcccg taatgcagtg
     2161 tcgaaccggg tgaactgcac cgtggagtgc tgcggcgcgt catctcgcgc gatcgccacg
     2221 gcgccgccat agtcgccgcc ggccagcttg cgtcggatct cgtcatggga gcgcatccag
     2281 ccgtgcagcg cgtccgccgc cccgtcaacc cggtgctcgt cgagcagcca ccctatctgt
     2341 gcggtccgct catcgaatct gatgtcggac agggggtcgg agccccgttt gagcagtccg
     2401 aggacttctt ccgcccgggc ctgctgagcg aggatccgcg cgctaaccgc ggtgtccagc
     2461 ggcccaccgc catgcgtccg cgcactggtg gcggcatgag tcgacaccag cccggccact
     2521 gtcaaccaca ccacgagcac cgccatcagt aacgacgcca gcaccagacc ggggttgagc
     2581 cgacgatggc tgcgccgagc cagcgccgcc tgcgagagca ccagcaacac cagcacgacg
     2641 acggccgccg cgatcacgac acgaggcgga ccgacggagc gtcccgacgc gatgacggca
     2701 tcggattgac tcgcgtacaa ccgttcggcg gccggcagga tgccctgctg catcaacgcc
     2761 gacgattcgc tgaggtatgc gactccgatc gggctaccgg cgcggttctt ggcccgggca
     2821 gtggccacaa tcccggtgta caccgccagc tggttggaga tgtcagtgag cagcgtcagc
     2881 gagtgaatat cgttgggcga cacgccgttc gaggcggtga ccattccggc agacgcctgt
     2941 ccgatggcag cgtcgtagcg atcgcggacg tcgcgcggtt cgacgccgcc ggaaaggaat
     3001 gcggtggccg cggtcgtgtt ggcatacgag agagcgccgt agatccgttg tgccgcatcg
     3061 gccagcggtt cggtgtgcga tcgcagggtc tccagctggc cttgccggtc gaaaaccgtc
     3121 ttcgacgcgg tcgcaccgac ggcgagcagc gccacgacca gaacgacagc caccaacgca
     3181 atccggcccg gtgtggctct gaggaagtcg acgaccggcc ggtctgattc ctcgggatcg
     3241 acgagcaggg actcgtcgag tgtgcgcgtc cgctggtgcg ccagctcatc gctgtgcact
     3301 cgacatcacc tcatcgcacg ttgggcctgg accagcctct atccagtgcc gatggatggc
     3361 acggtagccc tcggaaacgc attgcggggg tggttgccac actcagcatc ccgctcgcat
     3421 cgggcccttc cgttcctgcg gcccttgccg cgctggatgc ggcagagacc acgcgtcacg
     3481 accaagcgtc ggcgggatga tggtggaacc atcggaggtg accgatatgg atctttactt
     3541 gatgcagcac ggtcaggcga cgaccgaaac cgaggatcct gagcgaccac tcacggatgc
     3601 cggtcgagct gcggtgcagc gtgtcgctga aagagcccgg gccgccggcg tccagttcag
     3661 cggctgcctg cacagcggaa agctgcgcgc agagcagact gcgcacctgc tggtcaggga
     3721 gatcggcgtg gagccgagcg tcgaggcgcg accgggtctt gggccgaatg acccggtgat
     3781 accggtagcg cagtggctcc gaacgcggac cgagcacgag tcgcttgcgc tcgtgggtca
     3841 catgcctttc ctcggcaggc ttgcctcgtt gctggtcgta ggcgacgagc aggcccaggt
     3901 ggttggcttc cagatgggtg gcctggtgaa gctggaaccg aaggtagatc gggatgggtt
     3961 ctccgtggca tgggcgcttc cgcccgacct cgcctgacca tgcaccagaa cggcgccatg
     4021 catcacagag agtggccgcg atggcggcga cgggtccgat cagcggtgcc gtctcgctgc
     4081 agcgaacatc atggttcagg catttctgct gcagcggaac cgatttcggt ggtttcggca
     4141 cgcagatgat cccggccaca tgtggccgcg gcccggccgt tagacaccga caacctctcg
     4201 agactcagcg ccgagcgggg accatggcca gtcacttcgt atagtcca
//
LOCUS       DASRJA010000721        13770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3766928,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000721 DASRJA010000000
VERSION     DASRJA010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13770
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3766928"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..80)
                     /locus_tag="VFC01_32555"
     CDS             complement(<1..80)
                     /locus_tag="VFC01_32555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HZN84388.1"
                     /translation="MTVRLEQLYQEVILDHYKHPHHRGLR"
     gene            complement(77..1330)
                     /locus_tag="VFC01_32560"
     CDS             complement(77..1330)
                     /locus_tag="VFC01_32560"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HZN84389.1"
                     /translation="MTASAQTLDLTKIRADFPILKRVMRGGQQLAYLDSGATSQRPLQ
                     VLDAERDFLITSNGAVHRGAHQLMEESTDAYEQGRADIAAFVGADPDELVFTKNATEA
                     INLVAYVLGDNRFEHAVGPGDVIVTTELEHHANLIPWQELARRTGATLKWYGVTEDGR
                     IDLDSLTLDERVKVVAFSHHSNVTGALAPVGELVARAKAVGALTLLDACQSVPHQPVD
                     FHALGVDFAAFSGHKMLGPNGIGVLYGRGELLNAMPPFITGGSMIETVHMEATTYAPA
                     PQRFEAGTPMTSQVVGLAAAARYLQEIGMDAVEAHENELVAAALTGLSGIDGVRIIGP
                     KSLENRGSPVAFVVDGVHAHDVGQVLDDDGVAVRVGHHCAWPLHRRFGIAATARASFA
                     VYNTPDEVDRLVAGVRHAVEFFGRD"
     gene            complement(1332..2099)
                     /gene="sufC"
                     /locus_tag="VFC01_32565"
     CDS             complement(1332..2099)
                     /gene="sufC"
                     /locus_tag="VFC01_32565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005057836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HZN84390.1"
                     /translation="MTTLEIKDLHVSVVSKEDGTDIPILKGVNLTVKSGETHALMGPN
                     GSGKSTLSYAVAGHPKYTVTSGSITLDGEDVLEMSIDERARAGLFLAMQYPIEVPGVS
                     MSNFLRTAATAVRGEAPKLRHWVKEVKGAMEGLDIDPSFSERSVNEGFSGGEKKRHEI
                     LQLELLKPKIAILDETDSGLDVDALRVVSDGVNRYAETVHGGLLLITHYTRILRYIQP
                     QFVHVFVGGRIVESGGPELADELDANGYERFTQAVGA"
     gene            complement(2096..3280)
                     /gene="sufD"
                     /locus_tag="VFC01_32570"
     CDS             complement(2096..3280)
                     /gene="sufD"
                     /locus_tag="VFC01_32570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HZN84391.1"
                     /translation="MVAQDLTTAVEGVNKGEIFTSFDVDAFEVPHGRDEIWRFTPLKR
                     LRGLHDGSATATGQATVSVDAPDGVWVDTTTRDADSRLGQAGAPADRVAAQAFSSFEQ
                     ATVVEIPRDTQIREPIEIAVTGPGEGATAYGHLQVRVGELAEAVVVIDQSGSGTYADN
                     IEFVVGDAARLTVVSIADWADDAVHVSTHHAALGKDAVLRHVAVTLGGEVVRLTARTR
                     FCAPGGDAELLGLYFADDGQHFESRLLVDHAQPNCRSHVTYKGALQGDPESNKPDAHT
                     VWVGDVLIRAEATGTDTFETNRNLVLTDGARADSVPNLEIETGEIVGAGHASATGRFD
                     DEQLFYLQARGIPEDQARRLVVRGFFGELIQKIAVPAVRERLTDAIEHELEITESRTA
                     TS"
     gene            complement(3277..4722)
                     /gene="sufB"
                     /locus_tag="VFC01_32575"
     CDS             complement(3277..4722)
                     /gene="sufB"
                     /locus_tag="VFC01_32575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HZN84392.1"
                     /translation="MTTTPEVHKVGEPLSQDEAIASLSRYGYGWVDSDVAGASAQRGL
                     SEAVVRDISAKKNEPEWMLEVRLRALRTFNKKPMPNWGSNLEGIHFDNIKYFVRSTEK
                     QAATWDDLPEDIRNTYDKLGIPEAEKQRLVSGVAAQYESEVVYHQIREDLEALGVIFL
                     DTDTALKEHPEIFKQYFGTVIPAGDNKFSALNTAVWSGGSFIYVPKGVHVDIPLQAYF
                     RINTENMGQFERTLIIVDEGAYVHYVEGCTAPIYKSDSLHSAVVEIIVKPGGRCRYTT
                     IQNWSNNVYNLVTKRARAEAGATMEWVDGNIGSKVTMKYPAVWMTGEHAKGEVLSVAF
                     AGEGQHQDTGAKMLHLAPNTSSNIVSKSVARGGGRASYRGLVQVNKGAHGSRSSVKCD
                     ALLVDTISRSDTYPYVDIREDDVTMGHEATVSKVSEDQLFYLMSRGMTEDEAMAMVVR
                     GFVEPIAKELPMEYALELNRLIELQMEGAVG"
     gene            complement(4719..5504)
                     /locus_tag="VFC01_32580"
     CDS             complement(4719..5504)
                     /locus_tag="VFC01_32580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN84393.1"
                     /translation="MVSVTLRAPLLYRVSEFRHNSVVKFRPDTDRHTRGAIVQLLLES
                     GPITAGEIGEQLGISAAGVRRHLDALLEAGDALSSAAAAWQHHGRGRPAKRYRLTAEG
                     RAKLQHTYDDFAIAAMRQLREIGGDDAVRTFARRRIDSILSGVSEGSGDVEAKADRVA
                     TALTKAGYATTTTPVTGPIHGIQLCQHHCPVSHVAEEFPELCETEREAFAEILGTHVQ
                     RLATIVNGDCACTTHVPLTPEKPESLRRTARAEATTSKQGAST"
     gene            5529..7241
                     /gene="mptB"
                     /locus_tag="VFC01_32585"
     CDS             5529..7241
                     /gene="mptB"
                     /locus_tag="VFC01_32585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HZN84394.1"
                     /translation="MALRLRSLSTSIAGWHGDERAVGSPLNDAELIAFRRTRLFGATG
                     TVLMTIGALGVGARPVVQDPTFGVRLLNLPSRIQTVSLTMTTTGAVMMALAWLMLGRF
                     TLGERRMTRSQLDRTLLLWVLPLLIAPPMYSRDVYSYLAQSEISLQGLNPYRVGPATG
                     LGLDHVFTLSVPNMWRETPAPYGPLFLWIGQGISALTGENIVEAVLCHRLVVLLGVGL
                     IVWATPRLARRCGVAEVSALWLGAANPLLIMHLVAGIHNEALMLGLMLAGTEFALRGI
                     GAVGPLLPKPWAWPHGRDEWGRWLPMANLLLGVVLITLSSQVKLPSLLALGFVAVALA
                     YRWGGTFKAFVIAGGSLTAVSVVVMALIGWASGLGFGWVFTLGTANVVRSWMSLPTLL
                     ALGTGQVGILLGLGDHTTAVLGLTRAIGVFAIAIVVTFLLLAVLRGRLHPVGGLGVAL
                     GMTVLLFPVVQPWYVLWAVIPLAAWATRQQFRTATIAVTLVVGIFGPTANGDRFTLFQ
                     ILLAVVASTVIVLILIGLTYYRLPWRKLPEAVPPRVVEAPPIVEPAIPAPQAAKPGAY
                     AESP"
     gene            7238..8170
                     /locus_tag="VFC01_32590"
     CDS             7238..8170
                     /locus_tag="VFC01_32590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN84395.1"
                     /translation="MTSDSTVPVRLRGVSKRYGSTTAVSELDLDVHAAEVLALLGPNG
                     AGKTTTVEMCEGFIKPDAGSIEILGLDPVAENARVRERIGVMLQGGGAYPAARAGEML
                     NLVAAYAANPLDPQWLMHTLGLTEAARTTYRRLSGGQQQRLALACAVVGRPELVFLDE
                     PTAGMDAHARIVVWELINGLRRDGVTVVLTTHQLTEAEELADHIVIIDHGVAVATGTP
                     AELMRSGAENQLRFRAPRMLDLSLLVSALPESYKATETAPGEYLVEGHIDPQVLATVT
                     AWCARLDVLATDMRVEQRSLEDVFLDLTGRELRP"
     gene            8167..8940
                     /locus_tag="VFC01_32595"
     CDS             8167..8940
                     /locus_tag="VFC01_32595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN84396.1"
                     /translation="MTNLFAPGTFSPDPRPATVQKMLAAQFGLELKLLLRNGEQLLLT
                     MFIPITLLVGLTLLPLGSFGPNRAATFVPAIMALAVISTAFTGQAIAVAFDRRYGALK
                     RLGATALPVWGIIAGKSLAVVTVVFLQSILLGAIGFALGWRPSILGLVLGAAIIALGT
                     AGFAALGLLLGGTLRAEIVLAVANLLWFVFAGLGALTLEGGMVPHGVQWVARLTPSGA
                     LTEALTQAMTLSVDWFGIAVLAAWGGVAALCALRWFRFT"
     gene            complement(8908..9540)
                     /locus_tag="VFC01_32600"
     CDS             complement(8908..9540)
                     /locus_tag="VFC01_32600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84397.1"
                     /translation="MARPVLYPLRPVETATVRFTAAPHHRRRVTIDHRPLTDVTQEML
                     LDWFTHLGGTMSYGGEVIDRYLAWHPIDHIRWELSRPAPRGGAAEGARFRIVEAFGGR
                     PEFRVDVVDRVEKLDETGIRLVQRLAGIPIFQLEHTWSVGSDSVHYVSVMDIGARSAL
                     LSPVNSVLCRRFPDEMLRAWVKHNIEEVGQLEYLLPQLKSSGKAEPAQRA"
     gene            9628..10203
                     /locus_tag="VFC01_32605"
     CDS             9628..10203
                     /locus_tag="VFC01_32605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN84398.1"
                     /translation="MRRRRGDELDTAIRDAVLKLLATNGPAGVTMEAVAAAARTSKPV
                     LYRRWPDSGSLLRDTLLRMATAAIPHEDTGSYRGDMLAILRGWAALFSGPAAPAVQAL
                     IAAMAHDHELAAAFREDVIGWRKKEMAALLSRGIARGDVRADVPVEIARELGQSVLWH
                     RLLVTGDPIDDDLVVQLVDEVLVPFVSPRSS"
     gene            10417..11328
                     /locus_tag="VFC01_32610"
     CDS             10417..11328
                     /locus_tag="VFC01_32610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HZN84399.1"
                     /translation="MPSLRVQRVIAAAVILTQGGIAVTGAIVRVTASGLGCPTWPQCF
                     PGSFTPVPHAEIAVIHQVVEFGNRMITFLVVLTAALAVLAVTRARRRREVLIYAWLMP
                     ASTVVQAVIGGITVLTGLLWWTVAIHLLASMTMVWLAVLLYVKIGEPDDGISTPVAPK
                     PLRQLTAMTAVVLAAQLVSGTLVTGAGPHAGDKSITQPVPRLQVEITTLVHLHSSLLV
                     AYLTLVVGLGFGLLAVHAPRSVMLRLGVLLALVLVQGLVGTVQFFTGVPAALVAVHVL
                     GAAACTAATAALWASMRQRTKPEPLEG"
     gene            complement(11278..12180)
                     /locus_tag="VFC01_32615"
     CDS             complement(11278..12180)
                     /locus_tag="VFC01_32615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84400.1"
                     /translation="MLAGCPALPEGDGDDDGLVGALRQRGLHARWLSWDDPQTVDADL
                     VILRATWDYIDRLDEFLAWTTRVRNLLNPPQVVVWNTDKRYLADLADAGVPTVPSWFY
                     ARGEQIRIPDADEVVVKPAIGAGSVGALRFSDPDEARAHAAALQAESRTVLVQPYDPR
                     IEDGETALVFLGGRQSHAFTKGPILPPPGQAPVFDESGTYADETLTPADPDFEVWDVG
                     HAALAAAAAHLRIGVNDVLYARVDIIGDRRDAQVLELELVEPSLGWRQLDEATRTRQQ
                     REFALGVESALERLGLGPLSHRRP"
     gene            12241..13212
                     /locus_tag="VFC01_32620"
     CDS             12241..13212
                     /locus_tag="VFC01_32620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HZN84401.1"
                     /translation="MKAIEVAETGGPEVLTYVDKDQPSPDPGEVLIKAEAIGVNFIDT
                     YHRSGQYPRELPFILGGEVCGTVEAVGEDVAALSVGDRVVTAQANGAYAEYALAPADF
                     CAYVPEGVSADVAAASLLKGMTAHYLIKSTYPVQQGDAVLVHAGAGGVGLILTQWATS
                     LAARVITTVSTPEKAALSRKAGAVEVLDYPDDPEEFGAKIKDLTDGGVAAVYDGVGAA
                     TFDASLASCAVRGTVALFGASSGPVPPFDPQRLNTAGSLFLTRPTLVHHTRTPDEFAW
                     RAGELLDAIASGTITVTVSEHYRLEDAEQAHRDLQGRKTVGSVVLVP"
     gene            complement(13209..>13770)
                     /locus_tag="VFC01_32625"
     CDS             complement(13209..>13770)
                     /locus_tag="VFC01_32625"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZN84402.1"
                     /translation="LSAHLAGATIAFYVLVYTLLLKRRTSQNVVWGGAAGCMPVMIGW
                     SAVTGTIQWPALLMFAIIFFWTPPHTWALAMRYKEDYQAAGVPMLPAVATERQVTKQI
                     LVYTWLTVLATLVLALATGWLYASVAILAGAWFLVMAHQLYAGVRRGEPVKPLRLFLQ
                     SNNYLAVVFCALAVDSALALPTLFTA"
BASE COUNT         2156 a         4610 c         4553 g         2451 t
ORIGIN      
        1 tcacgcagcc cccggtgatg cgggtgcttg tagtgatcga ggatcacttc ctgatagagc
       61 tgctccagcc gcaccgtcag tccctgccga agaactcgac cgcgtgccgc acgccggcca
      121 cgagccggtc gacctcgtcc ggcgtgttgt agaccgcgaa cgacgcccgc gccgtcgcag
      181 cgatgccaaa ccggcgatgc agcggccatg cacagtggtg acccacccgc accgccacac
      241 cgtcatcatc gagtacctgc cccacgtcgt gagcgtggac gccgtcaacc acgaatgcga
      301 ctggcgaacc gcgattttca agtgatttcg gcccgatgat ccgcacccca tcgatgccgg
      361 acagcccagt caatgcggcc gcgaccaact cgttctcgtg cgcttcgacg gcgtccatcc
      421 cgatctcttg cagatacctc gcggcggcgg ccagtccgac gacctgtgag gtcatgggtg
      481 tgcccgcctc gaaccgttgg ggcgcaggag cgtaggtggt ggcctccatg tggacggtct
      541 cgatcatcga gccgccggtg atgaacggtg gcatcgcgtt cagcagctcg ccgcgaccgt
      601 agagcacgcc aatgccgttg gggcccagca tcttatgtcc cgagaacgct gcgaagtcga
      661 cgccaagcgc gtggaaatcg accggctgat gcggcaccga ctggcaggca tccagcaggg
      721 tcagcgcacc gaccgccttc gctcgcgcga ccagctcgcc caccggggcc agcgcgccgg
      781 tgacattcga atggtggctg aacgcaacta ctttcacgcg ttcgtctagt gtcagcgagt
      841 ccaggtcgat gcggccgtct tccgtgacgc cgtaccactt caacgtggca ccggtgcgac
      901 gggccagctc ctgccaaggg atcaggttgg cgtggtgctc cagctcggtg gtgacgatga
      961 catcgccggg cccgacggcg tgctcaaacc tgttgtcgcc cagcacgtag gcgaccagat
     1021 tgatcgcctc ggtggcgttc ttggtgaaca ccaactcgtc cggatcggca cccacgaacg
     1081 ccgcgatgtc cgcacgcccc tgctcgtatg cgtcggtgga ttcctccatc aactggtggg
     1141 ctccgcggtg cactgcgccg ttggaagtga tgaggaagtc ccgctcggcg tcgagcacct
     1201 gaagcgggcg ctgcgacgtc gcaccggaat ccaggtacgc caactgctgt ccgccgcgca
     1261 tcacccgttt caggatcggg aaatccgccc tgatcttggt gagatccaac gtctgcgccg
     1321 acgcggtcat gtcaggctcc gaccgcctgg gtgaagcgct catatccgtt ggcgtcaagc
     1381 tcatcggcga gctcgggacc gcctgattcg acaatgcgac cgccgacgaa tacgtgcacg
     1441 aactgcggct ggatgtagcg caggatgcgg gtgtagtgag tgatcagcag caacccgcca
     1501 tgcaccgttt ccgcgtagcg gttcaccccg tcgctgacga cccgcagcgc gtccacgtcc
     1561 agaccggagt cggtctcgtc cagaatcgca atcttcggct tgagcagttc gagctgaagg
     1621 atctcgtgtc gcttcttctc accaccggaa aagccttcgt tgacactgcg ttcggagaac
     1681 gacgggtcga tatcgaggcc ctccatcgcc cccttgacct ctttgaccca gtgccgcagc
     1741 ttcggcgcct cgccgcgtac ggcggtggcg gcggtgcgca ggaagttcga catcgacacc
     1801 ccgggcacct cgatggggta ctgcatcgcc aagaacagtc cggcacgagc gcgctcgtcg
     1861 atactcatct cgaggacgtc ctccccgtcc agcgtgatcg aacccgacgt caccgtgtac
     1921 ttcggatggc ctgccaccgc gtaggacagc gtcgactttc cggacccgtt gggacccatc
     1981 aaagcgtgcg tctcccccga tttcacggtg agattgacgc ccttgaggat cggaatgtcg
     2041 gtcccgtctt ccttggagac gacgctgacg tgcaggtcct tgatttccag tgtggtcatg
     2101 aggtggctgt tctcgattcc gttatctcta gttcgtgttc gatggcatcg gtgaggcgct
     2161 cgcgcaccgc gggaacggcg atcttctgaa tcagctcgcc gaagaagcca cggacgacga
     2221 ggcgacgggc ctggtcctcg gggatgccgc gggcctgcag ataaaacagc tgctcgtcgt
     2281 cgaaacgtcc ggtggcactg gcgtgtccgg cgccgacgat ctcgccggtc tcgatctcga
     2341 ggttcggcac cgagtcggcg cgggcgccgt cggtgagcac caggttgcgg ttggtctcaa
     2401 atgtgtcggt gccggtggcc tcggcccgga tcagcacgtc gccaacccac acggtgtgcg
     2461 cgtcgggctt gttcgactcc ggatcaccct gcagcgcacc cttgtaggtc acgtgtgagc
     2521 gacagttcgg ctgagcgtga tcgaccagca gtcgcgattc gaagtgctga ccgtcgtcgg
     2581 cgaagtagag gccgagcaat tcggcgtcgc cgccgggcgc gcagaaccgg gtccgcgccg
     2641 tcagccgcac cacctcgccg ccgagtgtca ccgcgacgtg gcgcagcacc gcgtccttgc
     2701 ccagcgccgc gtgatgcgtg ctgacgtgca cggcgtcgtc ggcccagtcg gcgatcgaca
     2761 cgaccgtcag ccgggccgca tcgccaacca cgaattcgat gttgtcggcg taggttccgc
     2821 tgccactctg atcgatgacg acgacggcct cggccagctc gccgacccgc acctgcaggt
     2881 gtccgtacgc cgtcgcaccc tcgccgggcc cggtgacggc gatttcgatc ggttcgcgga
     2941 tctgcgtgtc gcggggaatc tcgacgacgg tcgcctgctc gaacgacgag aacgcctgag
     3001 cggcaacgcg gtcggcgggc gcaccggcct gcccgagccg cgagtccgcg tcgcgggtgg
     3061 tggtgtcgac ccagacaccg tcgggcgcat cgacgctgac ggtggcttga ccggtggccg
     3121 ttgcggagcc gtcgtgcagg ccgcgcagcc gcttcaatgg ggtgaaccgc cagatctcgt
     3181 cacggccgtg cggcacctcg aaggcgtcga catcgaacga cgtgaagatc tcgcccttgt
     3241 tgactccctc gactgcggtt gtcaggtcct gtgccactat ccgaccgcgc cttccatctg
     3301 cagctcgatc aaccggttga gctccagcgc gtactccatc ggcagttcct tggcgatcgg
     3361 ctcgacgaag cctcgcacca ccatggccat cgcctcgtcc tcggtcatgc cgcggctcat
     3421 caggtagaac agctgatcct cgctgacctt ggacacggtg gcctcgtggc ccatcgtcac
     3481 gtcgtcctca cggatgtcga cgtacgggta agtgtcgctg cgactgatcg tatcaacaag
     3541 cagcgcatcg catttcacgc tcgagcgcga cccgtgcgca cccttgttga cctgaaccag
     3601 gccgcggtag gacgcgcgac cgccgccgcg ggccaccgac tttgacacga tgttgctcga
     3661 cgtgttcggc gccaggtgca gcatcttcgc gccggtgtcc tggtgctgac cctcaccggc
     3721 gaacgccacc gagagcacct cgcccttggc gtgctcgccg gtcatccaca cggccgggta
     3781 cttcatcgtg accttggacc cgatgttgcc gtcgacccac tccatggtgg cgccggcctc
     3841 ggcacgagcg cgcttggtga ccaggttgta gacgttgttg gaccagttct ggatggtcgt
     3901 gtaacggcaa cggccaccgg gcttcacgat gatctcgacc acggccgagt gcagcgaatc
     3961 gctcttgtag atcggcgcgg tacagccctc gacgtagtgc acgtaggcgc cctcgtcgac
     4021 gatgatcagc gtgcgctcga actggcccat gttctcggtg ttgatccgga agtaggcctg
     4081 cagcgggatg tcgacgtgca cgcccttcgg cacgtagatg aacgaaccac cggaccacac
     4141 agcggtattc agggcggaga acttgttgtc gcctgccggg atcacggtgc cgaagtactg
     4201 cttgaagatc tccgggtgtt ccttcagtgc ggtgtcggtg tcgaggaaga tgacgccgag
     4261 cgcctccagg tcctcgcgga tctggtggta gacgacctcc gactcgtact gcgcggcaac
     4321 gcctgacacc agccgctgct tctcggcctc ggggatgccg agcttgtcgt aggtgttgcg
     4381 gatgtcctcc ggcaggtcat cccacgtcgc agcctgcttc tcggtggagc gcacgaagta
     4441 cttgatgttg tcgaagtgaa tgccctcgag gttggagccc cagttcggca tcggcttctt
     4501 gttgaacgtc cgtagcgcgc gcagccggac ctcgagcatc cactccggct cgttcttctt
     4561 cgccgagatg tcacgcacca ccgcttcgga cagcccgcgc tgcgcgctgg cgcccgcgac
     4621 gtcggagtcc acccagccgt agccgtaccg gctcagcgag gcgatggcct cgtcctggct
     4681 gagcggttcg cccaccttgt ggacctcggg agtggtcgtc atgtcgacgc tccttgcttg
     4741 cttgtggtgg cttcggcgcg ggctgtgcgc cgaagtgatt ctggcttctc aggtgtgagc
     4801 gggacgtggg tggtgcacgc gcagtcgccg ttgacgatcg tcgccagccg ctgcacgtgg
     4861 gtgcccagga tctcggcgaa cgcctcccgc tcggtctcgc acagttcggg gaactcctcc
     4921 gcgacgtgtg acaccgggca gtggtgctgg cacagctgga tgccgtggat cggcccagtg
     4981 accggtgtcg tggtggtcgc gtagccggcc ttggtcagcg ccgtcgcaac cctgtcggcc
     5041 ttggcttcaa cgtcgcccga cccttcgctt actcccgaca ggatgctgtc gatccggcgc
     5101 cgcgcgaagg tgcggacggc gtcgtcgccg ccgatctccc gcagctggcg catcgcggca
     5161 atcgcaaagt cgtcgtaggt gtgctgcagc ttggccctgc cctcggcggt gagtcggtac
     5221 cgcttggcag gccgtccgcg tccgtggtgc tgccacgccg ccgcggcgct ggacagcgcg
     5281 tcgcctgcct ccagcagcgc atccaggtgc ctgcggacgc ccgcggcgga gatgccgagc
     5341 tgctcaccga tctcaccagc ggtgatgggt ccggattcca gcaagagctg gacgatcgcg
     5401 ccacgggtgt ggcgatctgt atccggacgg aatttcacaa cactattgtg acggaattcc
     5461 gaaacccggt aaagcaaggg tgcccttagc gtgacagaga ccatacgggt ggcggctacg
     5521 ctgctggggt ggcgctccgc ctgcggtccc tgagcacgtc gattgccgga tggcatggcg
     5581 atgaacgggc cgttgggtcc ccgctgaacg acgccgagct gatcgcgttt cggcgcaccc
     5641 gcctgttcgg cgccaccggc acggtgttga tgaccatcgg cgcgctgggc gtcggtgctc
     5701 gcccggtcgt gcaggacccg acgttcgggg tgcggctgct gaatctgcca tcacgtattc
     5761 agacggtgtc gctgaccatg acgaccacag gcgccgtgat gatggcgctg gcatggctga
     5821 tgctcggccg cttcacgctg ggcgagcggc gcatgacgcg cagccagctg gaccgcacac
     5881 tgctgctgtg ggtgctgccg ctgctcattg cgccacccat gtacagcagg gacgtctact
     5941 cctacctcgc gcagagcgag atctcgctgc aaggcctcaa cccgtatcgg gtcggcccgg
     6001 cgaccgggct gggccttgac cacgtgttca cgctgtcggt cccgaacatg tggcgagaga
     6061 ctcccgcgcc gtacggcccg ctgttcctgt ggatcggtca gggcatttcg gcgctcacgg
     6121 gcgagaacat cgtcgaagcg gtgctgtgtc atcgcctggt ggtattgctg ggcgtcggcc
     6181 tgatcgtctg ggcgacgccg cggctggccc gccgctgcgg agtcgccgag gtgagtgcgc
     6241 tgtggctggg cgcggcgaac ccgctgctga tcatgcatct ggtcgcaggc atccacaacg
     6301 aggcgctgat gctgggcttg atgctggcag gcaccgagtt cgccctgcgc gggatcggag
     6361 ccgtcggacc gctgctcccg aagccctggg cctggccgca tggccgcgac gagtggggac
     6421 ggtggctgcc catggccaac ctcctcctcg gtgtcgtgtt gatcacgttg tcctcacaag
     6481 tcaagttgcc gtcgctgctc gccctcggct tcgtggcggt ggcgctggca taccgctggg
     6541 gcggcacatt caaagcgttc gtgatcgcag gcgggtcgct gactgccgtc tccgtggtgg
     6601 tgatggcgct gatcggctgg gcgagcggcc tcgggttcgg ctgggttttc acgctcggca
     6661 ccgccaacgt cgtccgcagc tggatgtcgc tgcccacgct gttggcgctg ggcacaggac
     6721 aggtcggcat cctgcttgga ctgggcgatc acacgaccgc ggtgctcggc ctgacgcgtg
     6781 cgatcggcgt gttcgcgatc gcaatcgtgg tgacgttcct gctgctcgca gtgttgcgcg
     6841 gccggttgca tccggtgggt ggacttggcg tcgcgctggg catgacggtg ctgctcttcc
     6901 cagtggttca gccgtggtac gtgctgtggg ccgtcatccc gctcgccgcg tgggccacga
     6961 ggcagcagtt ccgcaccgcg accatcgccg tcacgctcgt ggtcggcatc ttcggcccga
     7021 cggccaacgg cgaccgattc acgcttttcc agatcctgct cgcggtggtg gcgagcacgg
     7081 tgatcgtgct catcctcatc ggactgacct actaccgcct gccctggcgc aagctgccgg
     7141 aggctgtccc cccgcgggta gtggaagcgc ctcccatcgt cgaacctgca ataccggcgc
     7201 cgcaggccgc caagcccggc gcttacgctg aatccccgtg acctccgact cgaccgtccc
     7261 cgtgcgcctg cgcggggtca gcaagcggta cgggtcgacg acggcggtct ccgaactcga
     7321 cctcgacgtc cacgcggcgg aagtgctcgc gctgctgggc cccaacggcg cgggcaagac
     7381 gaccacggtg gaaatgtgcg agggcttcat caagcccgac gctggcagta tcgaaatcct
     7441 cgggctggac ccggtggccg agaacgcccg cgtgcgtgag cgaatcggag tgatgctgca
     7501 aggcggtggc gcctatcccg cggcgcgggc cggcgagatg ctgaatcttg ttgcggctta
     7561 tgccgccaac ccactcgacc cgcagtggct gatgcacacg ctcgggctga cggaggcggc
     7621 ccgcacgacg tatcgacggc tgtcgggcgg ccagcagcag cggctggcgc tggcgtgcgc
     7681 ggtggtgggt cgccccgagt tggtgttcct cgacgagccc acagcgggca tggatgcgca
     7741 tgcgcgaatc gtggtgtggg agttgatcaa tgggctccgc cgcgacggtg tcacggtggt
     7801 gttgacgacc catcagctga ccgaagctga ggagctggcc gaccatatcg tgatcatcga
     7861 tcacggcgtc gcggtggcca ccgggacacc tgcggagctg atgcgcagcg gcgccgagaa
     7921 ccagctgcgc ttccgcgcgc cacggatgct cgacctctca ctgctggtgt cggcgctgcc
     7981 cgagagctac aaggcgaccg agaccgcacc gggtgaatac ctggtcgagg gccacatcga
     8041 ccctcaggtg ctggccaccg tgaccgcgtg gtgcgcgcga ctcgatgtgt tggccaccga
     8101 catgcgagtc gagcagcgca gcctggagga cgtgttcctc gatctgaccg gccgggagct
     8161 tcgaccatga cgaatctgtt tgcgccaggg acattctcgc cggatccccg tcccgcgacg
     8221 gtgcagaaga tgctggccgc gcagttcgga ctggaactga agctgctgct gcgtaacggc
     8281 gagcagttgc tgctcaccat gttcattccg atcacgctgc tggtcgggct gacgctgctg
     8341 ccgctgggct cgttcgggcc gaaccgtgcg gcgacgttcg tgccggcgat catggcgctc
     8401 gcagtgatct cgacggcctt caccggacag gccatcgcgg tggcgttcga ccgccgttac
     8461 ggggcgctga aacggctggg cgcgaccgcg ctgccggtct ggggcatcat cgccggcaag
     8521 tcgctggccg tcgtcacggt ggtgttcctg caatcaattc tgttgggcgc catcggcttt
     8581 gccctgggct ggcggccgag catcctcggg cttgtcctcg gggcagcgat catcgcgctc
     8641 ggcaccgcgg gcttcgcggc gctgggcctg ctgctcggcg gcacgctgcg cgccgagatc
     8701 gtgcttgcgg tcgccaacct gttgtggttc gtcttcgcgg gtctcggcgc gctgacgctg
     8761 gagggcggga tggtgccgca cggcgtgcag tgggtggccc gcttgactcc gtcgggtgcg
     8821 ttgaccgagg cgctgacgca ggcgatgact ctgtccgtgg actggttcgg gatcgcggtg
     8881 ctcgcagcgt ggggcggcgt cgccgcgcta tgcgcgttgc gctggttccg ctttacctga
     8941 cgacttcagc tgcggcagga ggtattccag ctggcccacc tcttcgatgt tgtgcttcac
     9001 ccaggcgcgc agcatctcgt cgggaaatcg ccggcataac acgctgttga ccggtgaaag
     9061 cagcgccgac cgcgccccga tgtccatcac cgagacgtaa tgcacgctgt cggaaccgac
     9121 agaccacgtg tgttcgagct gaaatatcgg aatgcccgcc aaccgctgca ccaaccgaat
     9181 gccggtctcg tcgagcttct cgacacggtc gacgacgtcg accctgaact ccggtcgccc
     9241 accgaaggct tcgacgatcc gaaagcgagc gccttcggca gcaccaccgc gcggcgccgg
     9301 gcgggaaagc tcccagcgga tgtggtcgat cggatgccac gccagatacc ggtcgatgac
     9361 ctcgccgccg taggacatgg tgccgccgag gtgggtgaac cagtcgagca gcatctcctg
     9421 cgtcacgtcg gtcagcggcc gatgatcgat cgtcactcgt cggcgatgat gcggggcagc
     9481 ggtgaaccgc accgtggcag tctcgaccgg gcgcagcgga tagagcacgg gacgggccat
     9541 tcaaactcct aagatacgcg atcgtttctt atcggctacg ctagactccg gctttaagat
     9601 acgcaagcgt ttcttcaagg gagatctatg cggcggcgcc ggggcgacga actggatacg
     9661 gcgatccggg acgcagttct caaactattg gcgaccaacg gtccagccgg cgtgaccatg
     9721 gaggccgtcg ccgcggcggc tcgcacgagc aagccggtcc tctaccggcg atggccagac
     9781 agcggaagcc tgctgcgaga taccttgctg cgcatggcaa ctgcggccat cccccatgag
     9841 gacaccggca gctatcgcgg cgacatgctc gccatcctcc ggggctgggc agccttgttc
     9901 agcggccctg ccgccccggc agtgcaagcg ttgatcgcgg cgatggcgca cgatcacgag
     9961 ctggccgccg cgttccgtga ggacgtgatc ggctggcgca agaaggagat ggcggcactg
    10021 ctttcgcgtg gcatcgcccg cggcgacgtg cgcgcggacg tccccgtcga gatcgcccgc
    10081 gaactcggcc agagtgtgct gtggcatcgg ctgctcgtca ccggcgatcc gatcgacgac
    10141 gacctcgtcg tacaactcgt tgacgaagtc ctggtgccgt tcgtctcgcc gcgaagttca
    10201 tgaccactgc gcctggcgcc gaccgatgac atcctcgatg ccgcaccgca atctggtact
    10261 gaaaatcgcg ctcacggtcg ttgcctcggg cggatcacga ccctgtgggc aatcttcagg
    10321 aacccttacc gctactacgg gctgtagttg cggtgctcta cgatcgaggc cgtgcccgtc
    10381 ggacgactct tcctgcggct ggtcgacctg ctgcccatgc cgagcctgcg ggtgcagcgc
    10441 gtcatcgccg cggccgtcat cctgactcag ggcggcatcg ccgtcaccgg cgcgatcgtc
    10501 cgcgtcaccg cgtcgggtct cggctgcccg acctggccac aatgcttccc gggcagcttc
    10561 acgccagtcc cccatgccga gatagctgtc atccatcagg tcgtcgagtt cggcaaccgg
    10621 atgatcacgt tcctcgtggt gctgacggct gcgctggcgg tgctggccgt cacgcgggcc
    10681 cgtcgccgtc gcgaggtgct gatctatgcc tggctgatgc ccgcgtcgac ggtggtgcag
    10741 gccgtgatcg gcggcatcac cgtgctgacc ggattgctct ggtggacggt cgccattcac
    10801 ctgctggcct cgatgaccat ggtgtggctg gccgtgttgc tgtacgtgaa gatcggggaa
    10861 cccgacgacg gcatatcgac gcccgtcgcg ccaaaaccgt tgcgacagtt gaccgcaatg
    10921 accgccgtcg tgctggccgc acagctcgtg agcggcacgc tggtcaccgg cgcgggaccg
    10981 cacgccggcg acaagagcat cacccaaccc gtgccgcggc tgcaggtcga gatcaccacg
    11041 ctggtgcact tgcactcgtc gctgctcgtc gcctacctca cgcttgtcgt cggtctgggc
    11101 ttcggtctgc tagccgtgca cgcaccccgc tccgtcatgc tgcggcttgg cgtgctgctg
    11161 gcgctggtac tcgtccaggg cctcgtcggc accgtgcagt tcttcaccgg tgtacctgcc
    11221 gcgctggtgg ccgttcatgt gcttggcgcg gcggcctgca ccgccgccac cgcggcgcta
    11281 tgggcgtcga tgcgacagcg gaccaagccc gagccgctcg agggctgact cgacgccgag
    11341 cgcgaattcc cgctgttgcc gggttctcgt cgcctcgtcc agttgccgcc aacccagcga
    11401 cggctccacc agctccagct cgagaacctg cgcgtcgcgt cggtcaccga ttatgtcgac
    11461 gcgcgcatac aacacatcgt tgacgccgat gcgaaggtga gcggccgcgg ccgcaagcgc
    11521 ggcatggccg acatcccaga cctcaaagtc gggatcggcc ggggtaagcg tctcatccgc
    11581 gtaggtcccg gactcgtcga acaccggcgc ctgtcccggc ggcggcagga tcgggccctt
    11641 ggtgaatgcg tgcgattgcc gcccgcccag gaacaccagc gccgtctcgc catcctcaat
    11701 tcgggggtcg tagggctgca ccagcaccgt gcggctctcg gcttgcaacg ccgcggcgtg
    11761 ggcgcgagcc tcgtcaggat cggagaacct cagggcgcca acggatcccg ccccgatggc
    11821 cggcttgacc acaacctcgt cggcgtcggg gattcggatt tgctcgccgc gcgcgtagaa
    11881 ccagctcggc accgttggca cgccggcgtc agccaggtcg gccagatagc gcttgtcggt
    11941 gttccacacg acgacctgcg gcgggttgag cagattgcgc acccgggtcg tccaggccag
    12001 gaactcgtcg agccgatcga tgtagtccca tgtcgcgcgc aggatgacca gatcggcgtc
    12061 aacggtctgc gggtcatccc aggaaagcca gcgggcatgc agtccgcgtt ggcgcagcgc
    12121 cccgaccagc ccatcgtcgt cgccatcgcc ctcaggcagt gccggacaac cggccaacat
    12181 gatgcgcgga tgaaagacgt cgggacgagc aagctttacc acgttggcag gatagtcagt
    12241 atgaaggcca ttgaagtcgc cgagaccggc ggacccgaag tcctgaccta cgtcgacaaa
    12301 gaccagccct cccccgaccc cggcgaggtg ctgatcaagg ccgaagccat cggcgtgaac
    12361 ttcatcgaca cctaccaccg gtccgggcag tatccacgcg agctgccgtt catcctcggc
    12421 ggcgaggtgt gcgggacggt cgaggccgtc ggcgaggacg tcgccgcact gagcgtcggg
    12481 gaccgcgtcg tcactgcgca ggccaacggc gcgtacgccg aatatgccct tgccccagct
    12541 gatttctgcg cctacgtgcc cgagggcgtc agcgccgatg tggccgccgc atcgctgctc
    12601 aagggcatga ccgcgcatta cctgatcaag tcgacgtacc cggtgcagca gggcgacgcg
    12661 gtactcgtac atgccggagc ggggggtgtg ggcctgatcc ttacgcagtg ggccaccagc
    12721 ctcgccgcgc gcgtgatcac caccgtgtcg acaccggaaa aggccgcgct gtcgcgcaag
    12781 gccggtgccg tcgaggtact cgattacccc gacgacccgg aggaattcgg ggccaagatc
    12841 aaagacctca ccgacggcgg tgtcgccgcg gtgtacgacg gtgtcggcgc ggcgacattc
    12901 gacgcgagcc tggccagttg cgcggtccgc ggcacggtcg cattgttcgg cgcatccagc
    12961 gggcccgtcc cgccgttcga cccgcagcgg ctcaacacgg ccggttcact gttcctgacc
    13021 cggcccacgc ttgtgcacca cacccgcact cccgacgagt tcgcttggcg cgcaggagag
    13081 ttgctcgacg cgatcgcctc aggcaccatc accgtcacgg tcagcgagca ctaccgcctg
    13141 gaagatgccg agcaggcgca ccgcgacctg cagggccgca agaccgtcgg ctccgtcgtg
    13201 ctggtgccct aggcggtaaa cagcgtcggc agcgccagag ccgaatccac cgccagcgca
    13261 cagaaaacca cggcgaggta gttgttggac tgcaggaaca gtcgcagcgg cttcaccggc
    13321 tcaccgcggc gcacgccggc gtacagctga tgggccatca ccaggaacca ggcaccggcc
    13381 agtatcgcca ccgacgcgta cagccagccc gttgcgagcg ccagcaccaa cgtcgccaac
    13441 accgtcagcc aggtgtagac caggatctgc ttggtgacct gccgctcggt ggcaaccgca
    13501 ggcagcatcg gcaccccggc cgcctggtag tcctccttat agcgcatcgc cagcgcccag
    13561 gtgtgcggcg gtgtccagaa gaagatgatc gcgaacatca gcagcgccgg ccattggatg
    13621 gtcccggtga ccgcggacca gccgatcatc acaggcatgc acccggccgc gccgccccag
    13681 accacgttct gtgaggtgcg gcgcttgagc agcagcgtgt acaccagcac gtagaacgcg
    13741 atggtggccc cggccagatg cgcggacagc
//
LOCUS       DASRJA010000722        12275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1470783,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000722 DASRJA010000000
VERSION     DASRJA010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12275
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470783"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1528)
                     /locus_tag="VFC01_32630"
     CDS             complement(44..1528)
                     /locus_tag="VFC01_32630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZN84403.1"
                     /translation="MSDVPELDADGLPEELSAFDQILHRGEAHPRTRSGIMTVELLDT
                     TPDWDSYRARFDTASRKVLRLRQKVVMPTLPTVAPRWVIDPDFNLDFHVRRLRVPEPG
                     TMRQVFDLAEVAAQSPLDISRPLWTATLVEGLEGGRAAQMVHLSHAVTDGVGGVEMFA
                     NLYDLERDPPPQAPPPLPVPQDLSPNDLMRQGINRLPITIAGRVRGMLFGAAHVVGEM
                     VRDPISRMGDVVEYAMSGARVVGPVAPPSPVLRRRSLSSRSEAIDIEFGALHKAAKAA
                     GGSINDAYLAGLCGALRLYHEAKGVPIEALPMAVPVNLRSEADPAGGNRFAGVNLAAP
                     VGLRDPEVRIKNIRSQMTRKREERAIDMVGAIAPVLTLMPDSVLESMAGSIVNSDVQA
                     SNVPVYAGDTFIAGAKVLRQYGVGPLPGVAMMVVLISRGGYCTISTRYDRASVIDSDL
                     WARCLVTGFDEVLALGGDGRAVPATFTIDALEPTPVSPNGSAAQ"
     gene            1895..1967
                     /locus_tag="VFC01_32635"
     tRNA            1895..1967
                     /locus_tag="VFC01_32635"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1928..1930,aa:Arg,seq:tct)
     gene            3242..4495
                     /locus_tag="VFC01_32640"
     CDS             3242..4495
                     /locus_tag="VFC01_32640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84404.1"
                     /translation="MKIRKSTTLMAGACTLCIAAASGTVEPQVQLAAHQESTVRVVSQ
                     DYTLAATVDPLAIAQLPFQNYFTIASTLGNLAAFIPPVNAATGAFFQGQWDKIPPLLQ
                     KAIADEIAAIGAVIQLPATIIAHDLAVITGAVGGTSSAALGNAVTQSDVATTAATPAD
                     TASGLLAIAQLPFQNYFTIASTLGNLAAFVPPVNAATGAFFQGQWDKIPPLLQKAFTD
                     ELAAINNVLQLPATIITTDLAVISGAFGAGLGATALTTGTSSIEMQALTAALSTDTNT
                     TTGTGTGDKNGSGTNDTVSNNTGAKNEDAGTNDTDTDTNDKNGSGINKTNTSTDTSST
                     TTGTTTTGATNTGTNDKNGSGTNDKNGSGINDTSTTNNGSGTKSVSATNSGSGATNAS
                     TTKSGAGTDDNGAGAKKPGNDTGAK"
     gene            4817..6187
                     /locus_tag="VFC01_32645"
     CDS             4817..6187
                     /locus_tag="VFC01_32645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE-PPE domain-containing protein"
                     /protein_id="HZN84405.1"
                     /translation="MSKTAANRVSRLARGVAAVGVATTVAGVAATQPASISASLVDLA
                     ALIVAGSSTNPTGAGVEDFFQGEFNAPPYNTGDDLVHVNFLTGPFGINLALQDHVGED
                     NAVLSSGWGAANASFLLMALQAQQDPVLSQTLFILDNNVARPDGGFGTRYPWFALIGV
                     NPFPSPTHTDALGVVDVGYQYDYNSNAPADVLNPVAAVNSLVAYLYHRLNQNEVDLPV
                     NADGSPSVTCGTANTCGITDNGAVLECPDAQCGAIPVGDRVAAYVTQRGNTTYVTYTS
                     DGLPLTNLIRDFVPFGDVIADLTAPVLTAIVDSAYPNGNPIPADPSQYQPATPFSSLS
                     QLATTIPSAIQQTLTAATGSEPKAASTTTLTADATTSDAKILTTTDEESKPLTNVVRD
                     SEKAVPQSTGNDGTSSKTSDPVASIDKSTREEPQETTQSEATQSGATSNEPKDDSGNS
                     AEADKS"
     gene            6207..6809
                     /locus_tag="VFC01_32650"
     CDS             6207..6809
                     /locus_tag="VFC01_32650"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005522367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN84406.1"
                     /translation="MATPTDYWNHNTAYHPWLLSIAAEHRGDVLDIGCGDGLLAQRLA
                     AVSRSVTGIDPDPAAITRAADRLASHRHVTVSQQGFQDFRAGECRFDLITLVATLHHM
                     DLRASLVKARDMLNPTGEIAVVGCPTNKTVRDWVWSAMCVPAARTCSWLHSETRNIGV
                     IVTEPQEGLDDIRRTVDDLIPGASVRRALYYRYLLRWRRR"
     gene            complement(6824..7504)
                     /locus_tag="VFC01_32655"
     CDS             complement(6824..7504)
                     /locus_tag="VFC01_32655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HZN84407.1"
                     /translation="MKRFVFGVLALSFVLAGCGGAASGASQAPKLVPDTALTASLLSA
                     DELNTMMGASGMTPHTPVSQMGDHRNLLPNLNCLGVWQVNEAPIYDSSHWKSVRQQLV
                     RVPDADEWNFLVVQSVVSYRTADAAREFFSESTDRWSKCTNHTLNIQLNGQALPRWHS
                     GDLTKTDTQLAMQYTRSTGDQTRSCQRALSVTANLILDIQACKPQQESPITQAADVAD
                     KIESKLPR"
     gene            complement(7509..8033)
                     /locus_tag="VFC01_32660"
     CDS             complement(7509..8033)
                     /locus_tag="VFC01_32660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84408.1"
                     /translation="MKYVGERVVAASVVAFGLGLTLAPSALADDVTVDDSGAGAVQVA
                     AGPTSENASPAAVQACGQFAEVLDGSSTYYGDFADAFEGSDYADPAVSSSNTVGRTAL
                     RQSAAVSMQAAQTPGLDPAIADPMRIWSLGATKLLVKMGLRIPGDSLNSTATEMNNQA
                     VKVQEACAAAGTHA"
     gene            8428..9111
                     /locus_tag="VFC01_32665"
     CDS             8428..9111
                     /locus_tag="VFC01_32665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MspA family porin"
                     /protein_id="HZN84409.1"
                     /translation="MLNRFAILASVCVAIPVAVAPLAQADPPPPPDPAVPVNATNPLP
                     PEGAVPSGPPGILDTPDGWHLEVVGSNETQLPVAPLTTAISSREYLVAGTFTGKITGG
                     GKTKLTGGSFEAGEQIGCGIISDETEINPGASFTPGIGIPFTGNATGAFGISLQGKVY
                     LKPGTVTTVAIDKKKFKGTTTRITITGVRIKTDQCAGQSFIRSYATLTSSTDNTDDVI
                     TYLGVTKAV"
     gene            9140..9802
                     /locus_tag="VFC01_32670"
     CDS             9140..9802
                     /locus_tag="VFC01_32670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MspA family porin"
                     /protein_id="HZN84410.1"
                     /translation="MLNRFATLAACSLIPLGTAPIALADPPADDVGPPPDTGVVASAE
                     PGVVTTPDGWVLTVVAKDETQLPVAPLTTAVSSREYLVGGTFTGTVTGSGKSSLSGGT
                     LEAGYQIGCGIELGQVRLIGQVGIGTSGSSITGGLIPTGVTFPISGTIEIHAKPGTVS
                     TVTVDKKDYKAAPVRVTLKDTHIKVDGCVGQSFLRSYATLTSATADTDDIVAYYGVTK
                     AV"
     gene            9811..10506
                     /locus_tag="VFC01_32675"
     CDS             9811..10506
                     /locus_tag="VFC01_32675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84411.1"
                     /translation="MVQHRKRNAVANIATAGLLAIGAAVAFSATAAADPPPPEPLPVP
                     GEPEPPAPPPGPTVPVIGAPLGPSGFGVLAQNGQPANPAALGAPPVNGLDQTTVLGQN
                     AVPSAPGADPGVVPNLNVFNNAYGPQQCLVPSAPGKCEQFDVAPGDENADVSRREWLG
                     RYIDMYRAGMLKGGLLGQMPQEQLGEPLPGTAPAPGTNVPPGLVQFQPDPPAPPGTPP
                     PPGAPPLVPPPPG"
     gene            10641..11843
                     /locus_tag="VFC01_32680"
     CDS             10641..11843
                     /locus_tag="VFC01_32680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN84412.1"
                     /translation="MQTDVISDTRAVVVSTPVVWFMALAAALGTSTIYPLQPAIADVA
                     GSLHASFATIGVALACGPLGYLAGLALLVPLVDRFAPRYVLSAQFGALAIALAVNAVA
                     GTPWLLGLIVGVIGAGSSVGAQLSSVAGRFANPRRRATVLGIVTSGISAGILAGRIAG
                     GWLNDEIGWRGMFLVFAAACAIVAALAFRVLPGAPGSVTSGYLASLVRLPGLYFRVAP
                     LRLAAGRGTLWFFAFCAVWAGLGVALSQPPFSYSSERIGLYAFAGLLGIVATRVAGAW
                     TDRVGARRIVLVGLVLALTATATLGACLSNAAVTLACLAVFDAGLFAAQVANQSTVLA
                     IDPAAPAQFNSAYMLVYFIGGTLGTAFGAAAVGWFGWAATTAIAAGAIAIAMLITVAA
                     RPKSRSLG"
     gene            11848..>12275
                     /locus_tag="VFC01_32685"
     CDS             11848..>12275
                     /locus_tag="VFC01_32685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN84413.1"
                     /translation="MSDPVLVQVDDRVALITVNDPERRNAVTAEISAALRAAVDGAEA
                     NLDVHALIVTGAGKAFCAGADLTALGAATEDGLRGIYDGFLAVANCALPTIAAVNGAA
                     VGAGLNLALAADVRIAGPAALFDPRFQKLGIHPGGGATWML"
BASE COUNT         2237 a         4131 c         3765 g         2142 t
ORIGIN      
        1 ggcagccgca tggtccgtgg attgggctga ccgttggccg aagtcattgc gccgcacttc
       61 cgttcggtga cacaggggtg ggttctaggg cgtcgattgt gaacgtcgct ggcacggctc
      121 gaccgtcgcc gccgagggcg agcacctcat cgaagccggt caccaggcag cgagcccaca
      181 ggtcggagtc gatgaccgac gcgcggtcgt agcgtgtgct gatcgtgcaa tagccgccgc
      241 gtgagatcag cacgaccatc atcgctacac ctggcaacgg gccaacaccg tactgccgca
      301 agactttcgc gcccgcgatg aaggtatcgc ccgcgtatac cggcacgttg ctggcctgga
      361 cgtcggagtt gacgatcgac ccggccatcg attcgagcac ggaatccggc atcagcgtca
      421 gcaccggtgc gatggcgccg accatgtcga tcgcccgctc ctcgcgcttg cgcgtcatct
      481 gcgaacggat gttcttgatc cgcacttccg ggtcgcgcag gccaaccggc gcagcgagat
      541 tgacaccggc gaatcggttg ccgccggccg gatctgcctc agagcgaagg ttgaccggca
      601 ccgccatcgg cagcgcctcg atcggcacgc ccttggcttc gtggtacagg cgcaaggcac
      661 cgcacaggcc ggccaggtag gcgtcgttga tcgagccgcc agcggctttg gcggccttgt
      721 gcaaagcgcc gaattcgatg tcgatcgcct cgctgcgcga ggagaggctg cgacgtcgca
      781 gcaccggcga cggcggagcg acgggaccga cgacacgggc accggacatc gcgtactcga
      841 cgacgtcgcc cattctcgat atcgggtcgc ggaccatctc gccgacaacg tgcgctgcac
      901 cgaacagcat gccgcgcacc cgtcccgcga tcgtgattgg aagccggtta atcccttggc
      961 gcatcaggtc gttgggcgac aggtcctggg gaaccggaag cggcggaggc gcctgcggag
     1021 gcggatcgcg ctcgaggtcg tagagattgg cgaacatctc gacaccgccg accccgtcgg
     1081 tgacggcgtg gctgaggtgc accatctgtg cggcgcggcc gccttcgagg ccctcgacga
     1141 gggttgcggt ccacagcggt cgggagatgt cgagtggtga ctgtgcggct acctcagcca
     1201 ggtcgaacac ctggcgcatc gtgccgggct cggggacccg cagccgtcgc acgtgaaagt
     1261 cgagattgaa atcgggatcg atcacccatc gcggcgccac ggtgggcagc gtcggcatga
     1321 cgaccttctg ccgcagccga agaaccttgc gtgacgcggt gtcaaacctc gcccggtagc
     1381 tgtcccaatc cggggtggtg tcgagcagct cgacggtcat gatgccggag cgggtgcgcg
     1441 ggtgtgcttc gccccgatgc agtatctggt cgaacgcgct cagttcctcc ggaagtccgt
     1501 ccgcgtcgag ctccggcacg tcgctcatgc tcgtgctccc ctccttacgc cgaagtgctg
     1561 ccaccacgct agtccgctgc ccggcggcac ggagcgcctt tatatagctg ttgcggtcgc
     1621 aggcttcgcg cggcggagcg gcgccattca tgcggaagtt cggcgggcgc gatggctatg
     1681 gtccagcggg cgccgggctc agcacaccgt ttgaggactg caagcccgct gggggccagc
     1741 ggcgaactgc gcgcggcagc tcgcaaaatg cggccgcatc caacactcct gctagccggc
     1801 cattcttgct gggcgtaatc cggagagaga ccgacgcgcc atgagcatct ggccggccat
     1861 cggtccgcgc tgattcggct agggtgggtc cgctgccccc gtagctcagc ggacagagca
     1921 gccgccttct aagcggtagg tcgcaggttc aattcctgcc gggggtactg ccgggggcgc
     1981 tctctgacat gtctacggac atcagaatag ttgcgaaccc gcgttaggtc cggaaccttt
     2041 tcctcttcgg gcttttggtg ctccggtcga gttggtctta ctggggtcgc gggttagccg
     2101 cggaggagct caccggatgg cgagatgccg tgagctatca gccaatagca atgccagcgg
     2161 tgacgagcat ggccgcctca gcgctcgcgg cgagaagccc cttgttgatc atcgaccttg
     2221 cccggaccaa gggaagtagc tgtcctcgac tcgcggtgta atcgcagcat tcttcaagcg
     2281 gcgcaacggc aaacccgcaa ctactgacat cgccgcaaag tggcggggga cggtcgcttc
     2341 acgtttagtc ggcgctaggt tgctacgaaa actcgagggg ggccgccgga agtgagcaaa
     2401 accgctgcga atcgcgtgag cagactggcg atgggtgtcg cggccgccgg aattgcgacg
     2461 accgtcgccg ccgtagaccg gaagccaacc atcaagaaag agctcattac ggtggcggag
     2521 cgaaagtgtt gaccaatgtg gtgcgggaca gccgggacga cactcctgca atctcttccg
     2581 ttaccacgtt tgacaacaca acgaacggcg agcagcggga gatgtcgtaa tcgcatcgct
     2641 ttcgcccacc atccctccca gaccaattgg ccccatcgag ttgattcgcg aaatcgatgg
     2701 actgtatcga cagtgtaaca attttacttc ccaattggac gacgaactgg cgttccattg
     2761 tgccgcctta ataccatcgc cgtaaaaccg ccccggggtt aggaaaagcg cgcgccgaac
     2821 cgatatcggg ggacgaacaa ttggcggtaa atagacccga cttgctgaat acctcattcg
     2881 cggtttagcg acgggcggcg caattgattg cggctgagct ataccgacat atccgcgccg
     2941 ctccggcatg ccgctcggga ttcgccatgg ccgggacatg ccatggtgtc cgttgcggga
     3001 caggggaaca cacgcgcggc cacggccgag cacgttcgcc atgcgcaccg ccaaggaccg
     3061 cctcggtcat ttggtgaacg gcaggcaaaa tccagtttgc gcgtttgcat ccttcgctgg
     3121 tcgaagccat ccgtcgccaa aacgacggtg gaagggaccg ggtaattgct agtgaatcga
     3181 gccctcctca gttattttcg gcgtgtccaa ttctcgcctc tttctaccca caggggttca
     3241 cgtgaaaatt cgcaagtcca ctacgctgat ggctggagcc tgcacgcttt gcattgcggc
     3301 agcctcggga acagtcgaac cgcaagtcca gctggccgcc catcaggaaa gcaccgtccg
     3361 tgtggtctcc caggactaca cgctggcggc gacggtggat ccactagcga tcgcccagct
     3421 cccgttccag aactacttca ccatcgcgtc aacgctcgga aacctggcag ccttcattcc
     3481 gccggtcaac gcggccactg gcgcgttctt ccagggccag tgggacaaga tccccccctt
     3541 gctgcagaaa gccatcgccg acgagatcgc cgcgatcgga gctgtgattc agctaccggc
     3601 gacgatcatc gcccacgacc tcgccgtcat cactggtgcg gtcggcggca cgagcagcgc
     3661 tgcgctcggc aacgccgtga cccagagcga cgtggccaca accgccgcaa caccagctga
     3721 taccgcgagt gggctattgg cgatcgccca actcccgttc cagaactact tcaccatcgc
     3781 atcaacgctc ggaaacctgg cggcgttcgt ccctccggtc aacgcggcca ctggcgcgtt
     3841 cttccagggc cagtgggaca agatcccccc cttgctgcag aaagccttca ctgacgagct
     3901 cgccgccatc aacaacgtgc tccagctgcc tgccacgatc attacgaccg acctcgcggt
     3961 catttccggt gccttcggcg ccggcttggg cgctaccgcc ctgaccacgg gcacgagcag
     4021 catcgaaatg caggccctca cggcagcgct cagcaccgac accaacacca ccaccggcac
     4081 cggcaccggc gacaagaacg gttcgggcac caacgacacc gtcagcaaca acaccggcgc
     4141 gaagaacgag gacgccggca cgaacgacac tgacaccgac accaacgaca aaaacggttc
     4201 cgggattaac aaaacgaaca ccagcaccga cacctcgtca accaccaccg gcaccaccac
     4261 caccggcgcc actaacaccg gtaccaacga caaaaacggc tccggcacca acgacaagaa
     4321 cggctccggc atcaacgaca cgagcaccac caacaacggc tccggcacaa agagcgttag
     4381 cgccaccaac agtggctccg gcgccaccaa cgcgagcacc accaagagcg gggccggcac
     4441 cgacgacaac ggagccggcg ccaaaaagcc cgggaacgac actggcgcaa agtaacgatc
     4501 accgtgcacg cctgacggca aacagctata aggtcggcgt tgtggaccgt acggtccgca
     4561 acgccgatct cgtgtgtgat agaccctcgc tttccaaagt tgcggcacat ttggattagc
     4621 gatgggctcg ccgcggctca accaagaaac gctgcacccg ctcctcctgc cctacggctt
     4681 ctccgctgcg atatacggcc aatgggcttc tgggtgcgag tgacaaatcc gacccgctca
     4741 tcggatagcc ggcaactccc ctggcgccca ctcgccgcgg tatgttgcta ctgagagccc
     4801 ggagggggag ccggaagtga gcaaaaccgc agcgaaccgt gtgagcaggc tggcgagggg
     4861 cgtggcggcc gtcggcgtcg cgaccaccgt ggcgggcgtt gctgcgactc aacccgcctc
     4921 gatctcggcg tcgctcgtcg acctggcggc gctgatcgtc gccggcagct caacgaatcc
     4981 gacgggagcg ggtgtcgagg acttcttcca aggggagttc aatgcgccgc cgtacaacac
     5041 cggcgatgac ctcgtgcacg tcaacttcct caccggcccg ttcggcatca acctggccct
     5101 gcaagaccat gtcggcgagg acaacgccgt gctctcctcg ggctggggcg ccgccaacgc
     5161 cagttttctg ctcatggcgc tgcaggccca gcaggatccc gtcctgtcgc aaaccctctt
     5221 catcctggac aacaacgtcg cccggcccga cggcggcttc ggcactaggt atccgtggtt
     5281 cgctttgatc ggcgtcaacc ccttcccgtc gccgacgcac accgatgccc tcggggtcgt
     5341 cgacgtgggc taccagtacg actacaactc gaatgcgccg gccgatgtgc tcaacccggt
     5401 cgccgccgtc aattcgttgg tcgcctacct ctaccaccgc ctcaaccaga acgaggtgga
     5461 tctgcccgtc aacgccgacg gatctccttc ggtgacttgc ggcaccgcaa acacatgtgg
     5521 catcactgac aacggcgcgg tgctcgaatg tcccgacgca cagtgcgggg cgataccggt
     5581 cggcgaccgc gtggccgcct atgtcacgca gcgcgggaac actacctacg tcacctacac
     5641 cagcgacggc ctgccgttga ccaacctgat acgtgatttc gtgccgttcg gcgacgtcat
     5701 cgccgatctg accgcgcccg tgctgaccgc gatcgtcgac tcggcctacc ccaacggcaa
     5761 tcccatcccc gcagacccga gccagtatca gccagcaaca ccattttcgt ctttgagcca
     5821 gctcgcgacg acgatcccga gcgccatcca gcagacactg acggcggcca ctggttccga
     5881 gccgaaggcg gcgtcgacga ccaccctcac cgccgacgcg acaacatccg acgcgaagat
     5941 cctcaccacg accgatgagg agagcaagcc gttgaccaac gtggtgcgag acagcgaaaa
     6001 ggccgtgccg cagtcgaccg gcaatgacgg cacttcatca aagacgtcgg atcccgttgc
     6061 cagcattgat aagtcgacga gagaagagcc gcaggagacg acacagtccg aggcgacgca
     6121 atccggcgcg acctccaacg agccgaaaga cgattccggt aattcagcag aggccgacaa
     6181 gtcctgaagc tggcacagcc ttaggtatgg cgacgccaac cgactattgg aaccacaaca
     6241 ccgcctacca tccgtggctg ctgtcgatcg ccgctgaaca ccgcggcgat gtcctcgaca
     6301 tcggttgcgg cgacggcctt ctcgcccagc ggctcgcagc ggtgtcgcgg tcggtgacgg
     6361 gtatcgaccc cgatcccgcg gcgatcaccc gcgcggccga ccggctggca tcacatcggc
     6421 acgtcacggt ctctcaacaa ggcttccagg acttccgggc cggcgaatgc aggtttgacc
     6481 tcatcacgtt ggtggcgaca ctgcatcaca tggatctgcg cgcctccttg gtcaaggcgc
     6541 gcgacatgct gaacccaacc ggagagatag ccgtggtcgg atgtccaacg aataagactg
     6601 tgcgcgactg ggtttggtcg gcgatgtgcg tgcccgctgc gcggacttgc tcctggctac
     6661 actcggagac tcgcaacatt ggcgtcatcg tcacggagcc gcaggagggt ctcgacgaca
     6721 tccgccgcac ggtcgacgac ctgataccgg gggcgtcggt gcgacgggcg ctgtactacc
     6781 ggtatctcct gcgctggcgc agacggtagc tcgaactccg ctcttatctg gggagtttcg
     6841 actcgatctt gtcggcgacg tcagcggctt gcgtgatcgg agactcctgc tgcggcttgc
     6901 acgcttgaat gtcgaggatc aagttcgcgg tgacagagag cgctcgctgg cacgacctgg
     6961 tctggtcgcc tgtacttcgt gtgtactgca tcgcaagctg tgtgtcggtc ttggtcaggt
     7021 cgccactatg ccacctgggc aacgcctggc cgttaagctg gatattgagc gtgtgattgg
     7081 tgcacttcga ccatcggtcc gtcgactcac tgaagaactc gcgcgccgcg tccgcagtgc
     7141 ggtaggagac gacggattgc acgacgagga aattccactc gtcggcgtcc ggtacccgaa
     7201 ccagttgttg gcgcaccgac ttccagtgac tggaatcgta gatcggggct tcgttgactt
     7261 gccaaacgcc caagcagttg aggttgggca ggaggtttcg atgatcaccc atctgcgaca
     7321 cgggcgtatg cggcgtcatt ccgctcgccc ccatcatcgt gttcaactca tcggcgctca
     7381 ggagcgacgc ggtaagtgcg gtgtccggca ccaacttcgg tgcttgcgat gcgcccgaag
     7441 cggcgccacc gcagccggcg agcacaaagc tgagggctag caccccaaag acaaagcgtt
     7501 tcaccgaatt acgcgtgcgt cccggccgca gcgcacgcct cttggacctt gacggcttgg
     7561 ttgttcatct cggtggcggt gctgttcagg ctgtcgccgg gaatccgcag ccccattttg
     7621 acgagcagct tggttgcgcc gagtgaccag atccgcatcg gatcggcgat cgccggatcc
     7681 aatcccggag tctgggcggc ctgcatggat acagcggccg attgccgcag cgcggtccgt
     7741 cccaccgtat tgctgctgct gacggcggga tccgcgtagt cggacccttc gaacgcgtca
     7801 gcgaagtccc cgtagtaggt cgaagagccg tcgagcacct cggcgaactg tccacatgcc
     7861 tgcaccgccg cgggactcgc attttccgat gtgggacccg cggcgacctg gaccgcgccc
     7921 gcccctgaat cgtcaacagt gacgtcatca gcgagagcac tcggagcgag cgtcaagcca
     7981 aggccgaacg cgacgacgga tgcggcaaca actcgctcgc ctacgtactt caacgtttcc
     8041 tccaagttag tcggtctcgc agggttgcca ggtgtccctt cacccgttgg caggccgtca
     8101 caccggccag ccacacccac gtcgacgagc cccgtccatt ctcgagggca gtaacccgaa
     8161 ctacggtcaa tgggtgagat gtcctaacgg tgaacaaatg cctaacggtc gctttccact
     8221 tgccgtccat tacctgtcct cgaactgtga acgtgcctgg ggctcagtgt gttacacgcc
     8281 acaccccgta gacgcccact ggatgggaat gcggcgtgaa ctgtttgtgg tccgttcacc
     8341 gtttggcaac ccactcatta gatgctccct cgcgacagat ccttttggat ccttggattc
     8401 cgacccacga gaggcgctat cccaaccatg ttgaaccgct ttgccattct ggcatccgtc
     8461 tgcgtcgcga taccggtcgc ggtcgcgccc ctcgcgcagg ccgatccccc tccgcccccg
     8521 gatcccgcgg tgccggtgaa cgccaccaat ccgctgccgc cggagggtgc tgttccgtcg
     8581 ggacctcccg gaatcctcga tacgccggac ggctggcacc tcgaagtggt cggaagcaat
     8641 gagacgcagc tcccggtcgc cccgctgacc accgcgatct cgtcgcgtga gtacctggtg
     8701 gcaggcacct tcacaggcaa gatcacgggc ggcggcaaga ccaagctcac cggcggttcg
     8761 ttcgaggctg gagagcaaat cggttgcggg atcatctccg acgagacgga gatcaacccc
     8821 ggcgcaagct tcacgccggg catcggaatt ccgttcaccg gcaacgcgac gggcgcgttc
     8881 gggatcagcc tccagggcaa ggtctacctg aagcccggca cggtgaccac agtcgcgatc
     8941 gacaagaaga agttcaaggg cacgacgact cgcatcacga tcaccggcgt gcggatcaag
     9001 accgaccagt gcgccggcca gtcgttcatc cgctcgtacg ccacgctgac gagctcgacc
     9061 gataacaccg acgacgtcat cacctacctc ggcgtcacca aggccgtcta actcctgcga
     9121 actcgaacgg aacacaacca tgttgaatcg cttcgccacg cttgccgctt gctcgctgat
     9181 cccgctcggt accgcaccca ttgcattggc cgacccgccg gctgatgatg tcggaccgcc
     9241 tccggacacc ggagtcgtcg cctcggctga gcccggcgtt gtgacgacgc ccgatggctg
     9301 ggtgctgacg gtggtggcga aagacgagac ccagcttccg gtggcaccac tgacgaccgc
     9361 ggtctcgtcc cgtgaatacc ttgtcggcgg cacgttcacg gggacggtca cgggcagcgg
     9421 caagtcgagc ctcagcggtg gcacgctcga ggccggttac cagatcggct gcggcatcga
     9481 actcggccaa gtcaggctca tcggtcaggt cggtatcggc acctccggtt cctcaattac
     9541 cggtggcctc attccgacgg gcgtcacgtt cccgatctcc gggacgatcg agatccacgc
     9601 caagcctggc accgtctcca ccgtcaccgt ggacaagaag gattacaagg ccgcaccggt
     9661 gcgtgtcacc ctcaaggaca cccacatcaa ggtcgacggt tgcgtcggtc agtccttcct
     9721 gcggtcctac gcgacgctga cgagcgcgac agcagacacc gacgacatcg tcgcctacta
     9781 cggcgtgacc aaggcggtct gatcgcaggc atggttcagc acagaaagag aaacgcagtc
     9841 gcgaatatcg cgacggcggg tctgctcgct atcggagcgg cagtcgcctt ctcagcgacc
     9901 gccgctgcgg accctccgcc gcccgagccg ctgcctgttc ccggagaacc cgagccccca
     9961 gctcctccac ctggcccgac agtgccggta atcggcgcgc cgctggggcc gagcgggttc
    10021 ggggtgctgg ctcaaaacgg acagccggcc aaccctgccg cgctcggtgc gcccccggtg
    10081 aacgggctcg atcaaacgac ggtgctgggc cagaatgcgg ttccgtcagc accgggcgcc
    10141 gatcctggcg tggtgccgaa cctcaatgtg ttcaacaacg cctacgggcc gcagcaatgt
    10201 ttagtgccct cggcgcccgg caagtgtgag cagttcgatg tcgctcccgg cgacgagaac
    10261 gctgacgtca gccgtcgcga gtggctcggc cggtacatcg atatgtatcg cgccgggatg
    10321 ctcaagggcg gactgctggg ccagatgccg caggaacagc tcggtgaacc gctgccgggt
    10381 accgctcctg caccggggac caacgttccg cccggactgg tgcaattcca gcccgacccg
    10441 ccagcaccgc cgggcacacc tccgccacct ggtgcgccgc cgctggtgcc tccgccgccg
    10501 ggctgagggg ttttcagcga caacacatct gagcacgacg gggtcacatc cgcgagggtg
    10561 tggccctgtt tgcgtcggct ccaccgtcgt gtccgaattc cgccagcatc ggggacgctc
    10621 gccttcttag cgtcggtggc atgcaaacgg acgtcatctc cgacactcgc gccgtggtcg
    10681 tctcgacacc tgtcgtctgg ttcatggcac tcgccgccgc gctgggcact tccaccattt
    10741 atccccttca acccgcaatc gcggacgtgg cgggctcgct gcatgcatca tttgcgacga
    10801 ttggcgtcgc tctggcatgc ggaccgcttg gatacctcgc cggtctggcc ctgctcgtac
    10861 ccctcgtcga tcggtttgcg ccacgctatg tgctgtcggc gcaattcggt gcactcgcca
    10921 tcgcgttggc cgtcaacgct gtcgccggca caccatggct actcggcctc atcgtcgggg
    10981 tcatcggcgc tggatcgtcg gtaggcgcgc aattgagttc ggtcgcggga cgattcgcga
    11041 atccgcggcg acgcgccaca gtcctcggca tcgtaacctc ggggatctca gctggcatcc
    11101 tcgccggtcg aatagccggc ggctggctga atgacgagat cggctggcgt ggaatgtttc
    11161 tcgtcttcgc agccgcatgc gccatcgtgg cggccctagc tttccgcgta ctgcccggcg
    11221 caccgggaag tgtgacctcg gggtacctcg cgtcgcttgt caggctcccg ggcttgtatt
    11281 tccgcgtcgc gccgctgcga ctcgcagccg gccgtggcac cctctggttt ttcgcgttct
    11341 gtgcggtgtg ggcgggtctt ggcgtcgcgc tgtcgcagcc gccgttctcc tactcatcgg
    11401 agcgcatcgg actttacgcg ttcgccggac tgctcggcat cgtcgccacc cgtgtcgccg
    11461 gcgcgtggac agacagggtc ggcgcccgcc ggattgtcct ggtcggactc gtccttgcgc
    11521 tgaccgcaac ggcgacgctg ggcgcttgcc tgtccaacgc cgctgtcaca ttggcctgcc
    11581 ttgccgtgtt cgacgccggt ctgttcgccg ctcaggttgc caatcaaagc accgttctgg
    11641 cgatcgaccc ggccgcaccg gcccaattca acagcgccta catgctcgtt tacttcatcg
    11701 gcggcacgct gggcaccgcg ttcggcgcag cggccgtcgg gtggttcgga tgggccgcaa
    11761 ccaccgccat cgctgctggc gccattgcaa tcgccatgct catcacggtg gccgcacgcc
    11821 caaagagccg ctcactaggc tgaggccgtg tccgatccag tactggtcca ggtcgacgat
    11881 cgcgtcgccc tcatcacggt gaacgatcca gagcgtcgca acgccgttac tgcggagatc
    11941 tcggccgctc tgcgagccgc cgtcgacggc gcagaagcca acctcgatgt gcacgcgctc
    12001 atcgtgaccg gcgcaggcaa agcgttctgc gcaggcgcag atctcaccgc gcttggcgcc
    12061 gccacggagg acggcctgcg cggtatctat gacggttttc tcgcggtcgc caactgtgcg
    12121 ttgccgacga tcgcggcggt caacggagcc gccgtcggcg ccgggttgaa tctggcactg
    12181 gccgccgatg tccggatcgc cggtccggca gcgcttttcg acccgcggtt ccagaaactc
    12241 ggcatccatc ccggcggcgg cgccacgtgg atgct
//
LOCUS       DASRJA010000723         9204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3483770,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000723 DASRJA010000000
VERSION     DASRJA010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9204
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3483770"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFC01_32690"
     CDS             <1..681
                     /locus_tag="VFC01_32690"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HZN84414.1"
                     /translation="AVLVEERVAMRRELAALVARSPFGQGAAWPVVQTVQRDGICVEV
                     IAPAPGLDAELGSAAGQLALRLADELGVVGVLAVELFETVDDRLLVNELAMRPHNSGH
                     WSMDGARTGQFEQHLRAVLDYPLGDTSPIAPVAVMANVIGAQQTPTMSMDERLHHLFG
                     RIPEAKVHLYGKGERPGRKIGHVNILGAASGSLDDDAYVADVRERATRAAHWLSHAEW
                     TDGWSEHA"
     gene            763..1296
                     /gene="purE"
                     /locus_tag="VFC01_32695"
     CDS             763..1296
                     /gene="purE"
                     /locus_tag="VFC01_32695"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZN84415.1"
                     /translation="MTNARVGVIMGSDSDWSVMEEAAHALAEFDVPFEVGVVSAHRTP
                     GRMLDYARTAAGRGIEVIIAGAGGAAHLPGMVASATPLPVIGVPVPLAKLDGLDSLLS
                     IVQMPAGVPVATVSIGGARNAGLLAVRILGSGDPQVRSKVEAFQEGLAQTVLKKDTEL
                     RNRLLGGLTSPSNVTGE"
     gene            1309..2472
                     /locus_tag="VFC01_32700"
     CDS             1309..2472
                     /locus_tag="VFC01_32700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZN84416.1"
                     /translation="MAIGNPSFDAFKLPDEHNELRAVLRDLCEKEIAPYAADVDEKAR
                     YTDEALAALTASGFAAIHIPEEYGGQGGDSVAACIVIEEVARVCASSSLIPICNKLGT
                     MGLLIRGSEELKKQVLPSIASGEAVASYALSEREAGSDAASMRTRARHEGDEWVLNGA
                     KCWISNGGHSTWYTVMAVTDPDKGANGISAFVVHKDDPGFVVGAKEHKMGIKGSPTTE
                     LYFEDCRIPGDRIIGDPGTGFKTALATLDHTRPTIGAQAVGIAQGALDAAIAYVKERK
                     QFGKAIADFQAVQFMLADMAMKLEAARLMVYTAAARAERGEPNLGFISSASKCFASDV
                     AMEVTTDAVQLFGGYGYTTDFPVERFMRDAKITQIYEGTNQIQRVVMSRALLK"
     gene            complement(2571..2681)
                     /locus_tag="VFC01_32705"
     CDS             complement(2571..2681)
                     /locus_tag="VFC01_32705"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HZN84417.1"
                     /translation="MRHEQPGKWISHCHNAYNLGAGMAFFVEYAGFSRLT"
     gene            complement(2905..3618)
                     /locus_tag="VFC01_32710"
     CDS             complement(2905..3618)
                     /locus_tag="VFC01_32710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03089 family protein"
                     /protein_id="HZN84418.1"
                     /translation="MRQLTTLTTALLDPLMAADPMGPRITYYDDATGERIELSTVTLA
                     NWAAKTGNLLRDELGAGPGSRVAVLLPAHWQTAAVLFGIWWVGAEVVLNGDADVALCT
                     ADRLSDADDAVGAGEVAVLSLDPFGKPAADLPVGVTDYATSVRVHGDQISPERHPGPA
                     LDGRSVAEVLEAAQNRATAQGFTSSDRVMSTAGWTSADELTENLLSIFAAGASLVQVA
                     NPDEAALDRRRAMEKVTRG"
     gene            complement(3606..5081)
                     /locus_tag="VFC01_32715"
     CDS             complement(3606..5081)
                     /locus_tag="VFC01_32715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZN84419.1"
                     /translation="MPARLTRVIAVAVALAIVVGTGVAWGKIRSFESGINHVSPIALG
                     EGGEDGAIDILLVGLDSRTDAHGNPLSEEELATLRAGDDESTNTDTIILVRIPNNGKS
                     ATAISIPRDSYVQTPGGNKMKINGVYGSAYFEKKTELVEQKGVDPAQAEPEATEAGRE
                     ALIKTVANLTGVTVDHYAEIGLLGFALITDALGGVNVCLKDAVYEPLSGADFPAGWQK
                     LDGPQALSFVRQRHDLPRGDLDRVTRQQAVMASLAHDVISSKTLSSPATLNRLEDAVQ
                     RSVVLSDGWDIMEFAQQLQKLAAGSVAFATIPILEEAGWSDDGMQSVVRVDPTQVKEW
                     VASLLQEQDEGKTEQLAYTPSKTTVDVINDSDINGLAAAVSQVLTSKGFAAGSTGNND
                     GGHVTGSQVQAAKADDLGAQAVSKELGGLPIVENAQVSPGSARVVLAADYTGPGSGLD
                     GSDPTLSTVDPAAAGTTDSTDSEPAPSPILTAGANDPECVN"
     gene            5149..6021
                     /gene="rfbD"
                     /locus_tag="VFC01_32720"
     CDS             5149..6021
                     /gene="rfbD"
                     /locus_tag="VFC01_32720"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HZN84420.1"
                     /translation="MASRIVISGAAGMVGRSLAAVARRQGRDVLALTSAEWDITDPTA
                     AERFIQSGDVVINCAGYTKVDAAEADPQRAHAVNAVGPETIAHACAHAGADLIHISTD
                     YVFSGTARRPYEIDDETGPLSVYGRTKLAGELMVLAAMPDAHIVRTAWIYEGADGSDF
                     AAVMRRSAAGDGEVDVVADQVGSPTYVGDLVDALLQIADDGSIREPVLHAANDGAVSR
                     FEQAQAVFEAVGADPDRVRPVGTDRHPRPAPRPAYSALSARRSAEAGLTPLRPWRDAL
                     AAAMSSAGPLPSTP"
     gene            6061..6915
                     /locus_tag="VFC01_32725"
     CDS             6061..6915
                     /locus_tag="VFC01_32725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177952.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZN84421.1"
                     /translation="MVTVTYSPGPHLDRFLATLSHATERPVNVILADNGSTDGSPENA
                     VERYPNVRLLRTGGNLGYGTAVNRAVAEITPESEFVVVANPDVQWGPGSIDVLLEAAE
                     RWPRAGSLGPLIHDPDGTVYPSARHVPSLIRGGMHAVIGPFWKSNPWTAEYRQERTEP
                     SERPVGWLSGSCLLLRRSAFDEVSGFDERYFMYMEDVDLGDRLGKAGWQNVYVPSAEI
                     LHDKGHATGRDPARNLAAHHTSTYTFLADRHPQRWQAPLRWTIRAALAARAGLVVRNS
                     RRKQRRGR"
     gene            6915..7994
                     /locus_tag="VFC01_32730"
     CDS             6915..7994
                     /locus_tag="VFC01_32730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HZN84422.1"
                     /translation="MVNPAQVDAVVLVGGLGTRLRPLTLSAPKPMLPTAGLPFLTHLL
                     SRIAEAGIEHVVLGTSYKAAVFESEFGDGSKLGLQIDYVVEDEPLGTGGGIANVSGML
                     RHDTAVVFNGDVLSGADLRALLDSHRDSGADVTLHLVRVGDPRAFGCVPTDSEGVVTA
                     FLEKTQDPPSDQINAGCYVFKREVIERIPKGRALSVEREVFPGLLSDGLRVCGYVDST
                     YWRDMGTPEDFVRGSADLVRGIAPSPALAGQRGEKLVHDGAGVAPGALLIGGTVVGRG
                     AEIGAGARLDGAVIFDGVRIGAGAVIERSIIGFGARIGPRALIRDGVIGDGADIGARC
                     ELLRGARVWPGVTIPDGGIRYSTDV"
     gene            complement(7976..8527)
                     /locus_tag="VFC01_32735"
     CDS             complement(7976..8527)
                     /locus_tag="VFC01_32735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZN84423.1"
                     /translation="MSLHESAVAALTRWRAPDPAQDTLRHAMLAFLAARPDGCLRECA
                     PGHVTGSALVLDHSGTHALLTLHPRFGRWLQLGGHCEQTDTDIVAAALREATEESGID
                     DLTIDPEPAALHVHPVTCSLGVPTRHLDMQFIVHAPANAEIAVSDESLDLRWWPLDAL
                     PDDCDFGLTQLADSARKRQTSVE"
     gene            complement(8524..>9204)
                     /locus_tag="VFC01_32740"
     CDS             complement(8524..>9204)
                     /locus_tag="VFC01_32740"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HZN84424.1"
                     /translation="SDNGSNARTLLRPGEDDLFWLGTEEALALGRSQAQLLRRSVRRF
                     SDEPVAPELVERAVAEALTAPAPHHTRPVRFVWLQDPDTRLRLLDRMKDQWRADLTGD
                     GRPPEAVERRVSRGQILYDAPELVIPFMVPDGAHSYPDADRTAAEHTMFTVAVGAAVQ
                     ALLVALAVRGVGSCWIGSTIFAADLVRAELELPADWEPLGAIAIGYAEEASGPRDPVD
                     PDGLLVRK"
BASE COUNT         1451 a         2967 c         3233 g         1553 t
ORIGIN      
        1 gcggtgctgg tcgaggagag ggtcgccatg cggcgtgagc tggcggcact ggttgcacgg
       61 tcaccgttcg gacagggcgc ggcatggccg gtggtgcaga ccgtgcaacg cgacggcatc
      121 tgcgtcgagg tgatcgcgcc cgcgccgggt ctggacgccg aattgggcag tgcagcaggg
      181 caactcgcat tgcggctggc cgacgagctc ggcgtggtcg gcgtgctggc ggtagagctg
      241 ttcgaaaccg tcgacgacag gctcctggtc aacgaactgg cgatgcgtcc gcacaactcg
      301 gggcattgga gcatggacgg cgcgcggacc ggtcaattcg aacagcacct gcgggcggtg
      361 ctcgactatc cgctcggcga cacatcaccg atcgcgccgg tcgccgtgat ggccaatgtg
      421 attggcgcgc aacagactcc gaccatgagc atggacgagc ggctgcatca cctgttcggc
      481 cggatcccgg aagccaaggt gcatctgtac ggcaagggtg aacggcccgg ccgcaagatc
      541 ggacacgtca acattctcgg cgcggccagc ggctcgctcg acgacgacgc gtacgtcgca
      601 gatgtgcgag aacgcgcgac ccgggccgca cactggttgt cgcacgccga gtggaccgac
      661 ggatggagtg aacatgcata gtcggacgac gatcaagcgg cgaggtacga gccgcgttcg
      721 tgggggcacc tcccacgcga agcgtagggg gagagtccga ccgtgactaa cgcgcgggtc
      781 ggcgtgatca tgggcagcga cagcgactgg tccgtgatgg aggaagccgc tcatgcgctg
      841 gccgaattcg acgtgccgtt cgaggtcggc gtagtgtccg cgcatcgcac gccaggccgc
      901 atgctcgact acgcccggac cgccgcaggt cgcggcatcg aagtgatcat tgcgggcgca
      961 ggcggcgcgg cacacctgcc cgggatggtc gcgtcggcga cgccgctgcc cgtaatcggt
     1021 gtgccggtgc cgctggccaa gctcgacggg ctggactcgc tgttgtcgat cgtgcagatg
     1081 cctgccggtg tgccagttgc gacagtgtcg atcggcgggg cccgcaacgc cggattgctg
     1141 gcggtccgca tcctcgggtc gggcgatcca caggtgcgct ccaaggtgga ggcattccag
     1201 gaagggttgg cgcagacggt gctgaaaaag gacaccgagc tgcgcaaccg cctgttagga
     1261 ggtttaacct cgccaagtaa cgttaccggc gagtagcgag gagtcgacat ggccatcggt
     1321 aacccgtcat ttgacgcttt caagctcccc gacgagcaca atgagctacg cgccgtgctg
     1381 cgtgatctct gcgagaagga gatcgccccg tacgcggccg acgtcgatga gaaggcccgg
     1441 tataccgacg aagccctagc agcgctaacc gcctccggct tcgccgcaat ccacattccc
     1501 gaggagtacg gcgggcaggg cggcgactcg gtggcggcgt gcatcgtcat cgaagaggtg
     1561 gcgcgggtgt gcgcgtcgtc gtcgctgatc ccgatctgca acaagctggg cacgatgggc
     1621 ctgctgatcc gcggctcgga ggaactcaag aagcaggtgc tgccgtcgat cgcgtccggt
     1681 gaggccgtcg cgtcctacgc gctgtccgag cgtgaagccg gcagcgatgc ggcgtcgatg
     1741 cggacccgag cccggcacga gggcgacgag tgggtgctca acggcgccaa gtgttggatc
     1801 tccaacggcg gccactcgac gtggtacacg gtgatggccg tgacggatcc ggacaagggt
     1861 gccaatggca tttcggcgtt cgtcgtgcac aaggacgatc ccggcttcgt cgtgggtgcc
     1921 aaggagcaca agatgggcat caagggatcg cccaccaccg agctgtactt cgaggactgc
     1981 cgcattccgg gcgaccgcat catcggcgac cccggtaccg gcttcaagac ggcactcgcc
     2041 acgctcgacc acacccggcc gacgatcggt gcgcaggcgg tcggcatcgc gcagggcgcg
     2101 ctcgatgcgg cgatcgcata tgtcaaggag cgcaagcagt tcggcaaggc gatcgccgat
     2161 ttccaggccg tgcagttcat gcttgccgat atggcgatga agctggaggc cgccaggctg
     2221 atggtgtaca ccgccgcggc gcgtgccgaa cgcggtgagc ccaacctcgg cttcatctcg
     2281 tcggcgtcga agtgcttcgc atccgacgtc gcgatggagg tgaccaccga cgccgtccag
     2341 ttgttcggcg ggtacgggta caccaccgac ttccccgtcg agcggttcat gcgcgacgcg
     2401 aagatcaccc agatctacga gggcaccaac caaattcagc gcgtcgtcat gtcgcgtgcg
     2461 ctgctgaagt agtaggtgct gcggccggct atgtgtatga aagcgcccgc gtagcgagcg
     2521 actgggcggc tgtcgcttcc tacgcggaca tttcttgggt atgtagttgg ctatgtgagt
     2581 cgcgaaaaac ccgcgtactc gacgaagaac gccatccccg ccccgaggtt gtaagcgttg
     2641 tggcagtggc tgatccactt gcccggttgt tcgtgtcgaa ttcgacctcg acggtctgct
     2701 tgggcggcac cagcaccgtg tctcgcgggg ccttgggcac gccgcggttc accacctgga
     2761 acatgtggcc gtgaaagtta acgggtgtcg caccctctgg gtgcgatttc gcacgcgctg
     2821 cctcgacacc agcagcggcg ccaacagcac tcaacaccgg ggattggcgc cgaccgagca
     2881 cgttctcagc gtggcacttc cctactagcc gcgcgtgact ttctccatcg cacgacgtcg
     2941 atcgagcgcg gcttcgtcgg ggtttgccac ttgcacaagt gaggcaccgg cagcgaagat
     3001 cgacagcagg ttctcggtga gttcgtcggc gctcgtccag ccggccgttg acatcacccg
     3061 atcgctggac gtgaaaccct gtgcggtagc ccggttttgg gcggcttcga ggacctcggc
     3121 caccgaccgg ccgtccagcg ccggtcccgg atggcgttcc ggcgagatct gatcgccgtg
     3181 cacgcggacc gacgtcgcat agtcggtgac gccgacaggc aggtcggcgg ccggcttgcc
     3241 gaacgggtcc agcgacagca ccgcgacctc gcccgcaccg acggcgtcgt cggcgtcgga
     3301 cagtcggtcg gcggtgcaca gcgcaacgtc ggcatcgccg ttcagcacga cttccgcacc
     3361 gacccaccag attccgaaca acaccgcggc cgtctgccag tgcgcgggga gcagcacggc
     3421 gacccggctg cccggtccgg cgcccaattc gtcgcgcaac agattgccgg tcttggcggc
     3481 ccagttggcc agggtgaccg tcgacagctc gatgcgctcc ccggtggcgt cgtcgtagta
     3541 ggtgatccgc gggcccatcg ggtcggccgc catcagcgga tccagcagtg ccgttgtgag
     3601 cgtggtcagt tgacgcactc cgggtcgttg gcgccggccg tgaggatcgg ggacggcgcg
     3661 ggttcggagt cggtcgagtc cgtcgtgccc gcggccgccg ggtcgacggt ggacagtgtc
     3721 ggatcgctgc cgtcgaggcc ggaaccgggt cccgtgtagt cagcggccag caccacacgc
     3781 gccgagccgg gtgacacttg cgcgttctcg acgatcggca gcccgccgag ctccttggaa
     3841 accgcttgtg cgccaaggtc gtcggccttg gccgcctgca cctggctgcc cgtgacgtgg
     3901 ccgccgtcat tgttgcccgt cgaacccgcc gcgaagccct tgctggtcag cacctgtgag
     3961 acggcggcgg ccagtccgtt gatatcggag tcgttgatca catcgaccgt ggttttgctt
     4021 ggcgtatatg ccaattgttc ggttttgccc tcatcctgct cttgcagcag gctggccacc
     4081 cactccttga cctgtgtggg atcaacccgc accacgctct gcatgccgtc gtcgctccag
     4141 ccggcctctt cgaggatcgg gatggtggcg aacgcgacgc tgcccgcggc cagcttctgc
     4201 agctgctggg cgaattccat gatgtcccaa ccatcggaca acaccacgga tcgctggacg
     4261 gcgtcctcga gccggttcaa tgtcgcgggg ctggacagtg tcttgctgga gatcacgtcg
     4321 tgggcgagcg aggccatcac ggcctgctgt ctggtgaccc ggtcgaggtc gccgcggggc
     4381 aggtcgtgac gttgccggac gaagctcagc gcctgcggac cgtccagttt ctgccagccg
     4441 gcagggaagt ctgctcccga aagaggttcg tacacagcat ctttcaggca gacgttgaca
     4501 ccgccgaggg cgtctgtgat cagtgcgaag ccgagcagcc cgatctccgc gtagtggtcg
     4561 acggtgacac cggtcaggtt cgcgaccgtc ttgatcaacg cttcgcgtcc ggcttcggtg
     4621 gcctccggtt cggcctgtgc cggatccacg cccttttgtt cgacgagctc ggtcttcttc
     4681 tcgaaatagg ccgagccgta gacgccattg atcttcatct tgttcccacc gggcgtctgg
     4741 acgtaggagt cgcgggggat ggagatggcc gttgccgact tcccgttgtt ggggatgcgc
     4801 accaggatga tcgtgtcggt gttggtggat tcatcgtcgc cggcccgcag cgttgccaat
     4861 tcctcttcgg acagcgggtt gccgtgggcg tcggtgcggc tgtccaagcc gaccagcaga
     4921 atgtcgatcg cgccgtcctc gccgccctcg ccgagcgcga tcggtgagac gtggttgatg
     4981 ccggattcga atgaccggat cttgccccac gcaacgccgg tgccgaccac gatggccagc
     5041 gccaccgcca cagcgatgac acgggtcagg cgagcgggca tctgcccagg ctacttgcga
     5101 gacaggcata gaccgggtag cgccggtcgg cgtgccagac tcgttactgt ggctagccga
     5161 atcgttatct ccggcgccgc cggcatggtg gggcgctccc tggccgccgt ggcgcgtcgg
     5221 cagggccgtg acgtgctggc gttgacgtcg gcagagtggg atatcaccga ccccacggcg
     5281 gccgagcgtt tcatccagtc cggcgacgtc gtgatcaact gcgcgggcta caccaaggtt
     5341 gacgccgccg aggcggatcc gcagcgcgcc cacgcggtca acgccgttgg gcccgagacc
     5401 atcgcgcacg cctgtgcgca cgccggcgca gacctgatcc acatctcgac ggattatgtg
     5461 ttttccggca ctgcgcgccg tccatatgag atcgacgacg agaccggtcc gctcagcgtc
     5521 tacggacgga ccaagttggc gggtgagctg atggtgctcg ccgccatgcc cgacgcgcac
     5581 atcgtgcgga ccgcgtggat ctacgaaggc gccgacggaa gcgatttcgc cgccgtgatg
     5641 cgccgatcgg ccgccgggga cggcgaggtc gacgtggtgg cggaccaggt cggctcaccg
     5701 acgtatgtcg gtgacctcgt cgacgcgttg ctgcagatcg ccgacgacgg atcgatccgt
     5761 gagccggtgc tgcacgccgc gaacgacggt gcggtcagcc gcttcgagca ggcccaagcg
     5821 gtgttcgagg cggtcggcgc cgatccagac cgggttcgcc cggttggcac ggaccgccat
     5881 ccccggccgg caccgcggcc ggcgtactcg gccttgtccg cgcgccgttc cgccgaggcg
     5941 ggactgacgc cgcttcgacc gtggcgggat gccctggccg ccgcgatgtc ctcggctggc
     6001 ccgctaccct ctacgccgtg agtgagggac tggtcggatc tggtgagtga cgaattagtt
     6061 gtggtcacgg tgacgtattc accggggccg catctcgatc ggttcctggc gacgctgtcc
     6121 catgccaccg agcgacccgt gaacgtgatc ctggccgaca acggttcaac cgacgggtcg
     6181 ccggagaacg cggtcgagcg gtatcccaat gttcggctgc tgcgtaccgg cggcaatttg
     6241 gggtacggca ccgcggtgaa ccgcgcggtc gctgaaatca cgccggagtc agagtttgtc
     6301 gtggtcgcca acccggacgt gcagtggggg cccggcagca tcgacgtctt gctggaagcc
     6361 gccgaacgct ggccgagggc ggggtcgctg ggaccgctga tccatgaccc cgacggcacg
     6421 gtctatccgt cggcgcgcca cgtgcccagc ctgattcggg gcggcatgca cgccgtcatc
     6481 ggcccgttct ggaagtccaa cccgtggacg gccgaatacc gacaggagcg aaccgagccc
     6541 agcgaacgcc cggttggctg gctgtccgga tcatgcttgt tgctgcggcg ctccgcgttc
     6601 gatgaggtgt ctggattcga cgagcgctat ttcatgtaca tggaagacgt cgacctgggt
     6661 gatcgcctcg gcaaggcggg ctggcagaac gtatatgtgc cgtccgcgga gattttgcac
     6721 gacaagggcc acgccacggg ccgcgacccg gcccgcaact tggcggccca ccacaccagc
     6781 acctacactt tcctggcgga tcggcatcca caacggtggc aggcgccact gcggtggacg
     6841 ataagggccg cgctcgccgc gcgtgcgggc ctggtggtcc gcaattcccg gcgcaagcag
     6901 aggagggggc gctagtggtc aatccggcgc aggtggacgc ggtcgtactg gtcggtggac
     6961 tcggcacccg gctgcggccg ttgacgctgt cggcgcccaa gccgatgctg ccgaccgcgg
     7021 gcctgccgtt cctgacccac ttgctgtcga gaatcgccga ggccggcatc gagcatgtcg
     7081 tgctgggcac gtcgtacaag gcggcggtgt tcgaatcgga gttcggcgac ggatccaagc
     7141 tcggcctgca gatcgactat gtcgtcgagg acgagccgct cggcaccggc ggcggtatcg
     7201 ccaacgtgtc ggggatgctg cgccacgaca ccgcggtggt tttcaacggc gatgtgctct
     7261 ctggcgctga tctgcgggca ctgctggatt cccaccgcga cagcggtgcc gacgtcaccc
     7321 tgcacttggt gcgcgtgggc gatccccgcg cgttcggctg cgtgcccacc gattccgagg
     7381 gagtggtgac ggccttcctg gagaagacac aggacccgcc gagcgaccag atcaacgcgg
     7441 gctgctacgt gttcaagcgc gaggtcatcg agcggattcc gaagggccgg gcgctgtcgg
     7501 tggagcgtga ggtgttcccg ggtctgctgt ccgacgggct acgggtgtgc ggctacgtcg
     7561 attcgaccta ctggcgcgac atgggcacgc ccgaagactt cgtgcgcggc tcggccgacc
     7621 tggttcgtgg catcgcgccg tcgcccgcgc tggccggtca gcgcggcgag aagctcgtcc
     7681 acgacggcgc gggcgttgcg cccggcgcgc tgctgatcgg cggcacggtc gtcgggcggg
     7741 gcgccgagat cggtgcgggc gcccgactgg acggtgcagt gatcttcgac ggtgtgcgaa
     7801 tcggcgcggg cgcggtgatc gagcgctcga tcatcggctt cggtgcccgc atcgggccgc
     7861 gcgcattgat tcgcgacggc gtgatcggcg acggtgccga catcggcgca cggtgcgagc
     7921 tcctgcgcgg tgcgcgggtg tggcccgggg tcaccattcc ggatggcggc atccgctact
     7981 cgaccgacgt ctgacgcttt cgcgccgagt cggcaagctg cgtaaggccg aagtcgcaat
     8041 catcgggtaa cgcgtccagc ggccaccagc gcaggtccaa agactcgtcg ctgacggcga
     8101 tttcggcgtt cgccggcgcg tgcacgatga actgcatgtc gaggtgacgc gtgggcacgc
     8161 ccagcgagca ggtgacgggg tgcacgtgca atgcggccgg ctcgggatcg atcgtgaggt
     8221 cgtcgatgcc ggattcctcg gtggcttcgc gcaacgcggc ggcgacgatg tcggtgtcgg
     8281 tctgctcgca gtgcccgccg agctgcagcc accggccgaa ccggggatgc agcgtcagta
     8341 gcgcgtgggt gccgctgtgg tcgaggacga gcgcggagcc ggtgacatgg cccggtgcac
     8401 actcgcgcag gcagccgtcg ggccgcgcgg cgaggaacgc cagcatcgcg tgccgcagcg
     8461 tgtcctgagc gggatcgggt gcgcgccacc gtgtcagcgc tgcgacagcc gactcgtgca
     8521 ggctcacttg cgcaccagta gcccgtcagg atcaaccgga tcgcgcgggc cggaggcctc
     8581 ctcggcgtag ccaatcgcta tggcgcccaa cggttcccaa tcggctggca gctccagctc
     8641 ggcacgtacc aggtcggccg cgaagatggt ggagccgatc cagcagctgc cgacgccacg
     8701 cacggcaagc gcgaccagca gcgcctgcac ggcggcgccg accgccaccg tgaacatcgt
     8761 gtgctcggca gcggttcgat cggcgtcggg ataactgtgc gcaccgtcgg gcaccatgaa
     8821 cgggatcacg agttcgggtg cgtcgtagag gatttggccg cggctgacac gacgttcgac
     8881 agcctccggc ggcctgccat ccccggtcag gtcggcgcgc cactgatcct tcatccgatc
     8941 gagcaaccgc aggcgcgtgt cggggtcctg caaccacacg aaccgcaccg gccgggtgtg
     9001 gtgtggcgct ggcgcggtga gcgcttcggc gaccgcgcgc tcgacgagct ccggcgcaac
     9061 gggctcgtcg ctgaatcggc gcaccgatcg gcgcagcagt tgtgcctggc tgcggccgag
     9121 cgcaagagct tcctcggtgc ccagccagaa caggtcgtct tcacccggcc gcagcagcgt
     9181 gcgcgcattg gatccgttgt cgga
//
LOCUS       DASRJA010000724         9279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3528222,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000724 DASRJA010000000
VERSION     DASRJA010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3528222"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            545..1795
                     /locus_tag="VFC01_32745"
     CDS             545..1795
                     /locus_tag="VFC01_32745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84425.1"
                     /translation="MIPASSKKRQQLLEQSNGWPVPGNDPMSDRPRWYHQLVDPTLQN
                     MFNAVNKDDLVIHDAVTGSGGERFLNNLTQHQWQDDGLAAGGLFDWVGESAAHDSTGR
                     AAETAHALEEYTSNKHNQLLNLPDTQGQSLGQVNPELTRDWARAFSPYMDDMVGMNTG
                     DSNGLFAPLDPDSSVEPANTRHLMSVLYSDHPPLDGGADAGAAKTASQILSESSQAHI
                     NSYLTAAAESVADRNAGEDNFAMQSAGKLQAAMDLGAYDERFDTTHDKFEAQKQAHEM
                     QSKLFDLGKDVIGEFPGGSTVGGLGGLMKDVIVGSDPVMPGVQSVAGRDMFPVQLHMA
                     QTLAQAGVGDPHFLEVVRGHLDANGHFNIPREDGTPDYRYFKNDINNYLGSVGQPGVI
                     NDMTQNYWNTYTQAIINAAPPQQP"
     gene            1822..2673
                     /locus_tag="VFC01_32750"
     CDS             1822..2673
                     /locus_tag="VFC01_32750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84426.1"
                     /translation="MIAATLSSGCDTNTTKSPSTASNSPAQLYPNWPPLLNDFRFHWS
                     AAPGIDITEGPAMVVRAYLESYNTAWFTLNLDNLFPGFRRVTPENQEPEGDFLWQLVQ
                     IRPLGSGYTKTAKDARPHFGYQALHFLELTPIENGFRAIVCSGDFAHFVESTARPGKF
                     VSIGDNEETAQPDGEGYSGVYPTQIELTQRDPRIAPNPPPQRGPAPAPVQDVFGDWYF
                     TGASSSYWGPSSDRTARDFPPPELKQRCDVAMPTPAAERIAMMTGFKDQPPPHGEAIP
                     GWPAKVQ"
     gene            2707..3069
                     /locus_tag="VFC01_32755"
     CDS             2707..3069
                     /locus_tag="VFC01_32755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN84427.1"
                     /translation="MTDVREQGLQVFRQLMPGAEVNLRDGKFGDELLEIGIDNVFGRL
                     WARDGLSRRDRSLVTLGILIALRATDELKAHVQIARNNGLTEDEIAEVIYHASGYAGF
                     PAADTANKIARDVFDETS"
     gene            complement(3146..3218)
                     /locus_tag="VFC01_32760"
     tRNA            complement(3146..3218)
                     /locus_tag="VFC01_32760"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3183..3185),aa:Thr,seq:cgt)
     gene            complement(3275..4483)
                     /locus_tag="VFC01_32765"
     CDS             complement(3275..4483)
                     /locus_tag="VFC01_32765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZN84428.1"
                     /translation="MAGVFSRLVGQDAVEAELVAAAQAARGDSAHNGAATGTMTHAWL
                     ITGPPGSGRSIAALCFAAALQCTSDGVPGCGQCRACTTTMAGTHADVRRIIPEGLSIG
                     VDEMRNIVQIASRRPGTGRWQIVLIEDADRLTEGAANALLKVVEEPPPSTVFLLCAPS
                     VDPEDIAITLRSRCRHVALVTPPVDAIARVLIETDGLPEDEASWAASVSGGHVGRARR
                     LATDELARDRRQRALGLARDAATPSRAYAAAEELVATAEAEARALTEDRNEAETEELR
                     TALGAGGTGKGTAGTLRGATGAIKDLERRQKSRQTRASRDALDRALIDLATYFRDALL
                     VSSGADSVQANHPDMTDRVAAMAAHASPEKLLRCIEAVLECREALAVNVKPKFAIDAM
                     VATVGRALRN"
     gene            4565..6199
                     /locus_tag="VFC01_32770"
     CDS             4565..6199
                     /locus_tag="VFC01_32770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN84429.1"
                     /translation="MTTEAIPIGRISAFVRWFVRTPWPVFTLGMLQADIIGALLVLGF
                     LRFGLPPEDRIQLQDLPAFNLAIFLGYLFLSFTIGSYLTLRMLIPVMRWQRRDTLLGA
                     RDPAATEMARTRALKMPFYRSVINVTNWLLGSIVFIVASWPVASKSAPVVAVATGLGA
                     TATAIIGYLQSERVLRPVAVAALRGGVPENFRAPGVIQRQVLTWVLSTAVPILAIVLA
                     VVASKVEILTAPADRLTTPILLLAIAALVIGLFGTVLVAMSIADPLRQLRWALGEVQR
                     GNYNAHMQIYDASELGLLQAGFNDMVRDLAERQRLRDLFGRYVGEDVARRALERGTEL
                     GGQERDVAVLFVDLVGSTHLASTRPAAEVVNLLNEFFRVVVDTVNRHGGFVNKFQGDA
                     ALAIFGAPIDHPDASGAALAAARELHDELVNVLGQTEFGIGVSAGRAIAGHIGAQARF
                     EYTVIGDPVNEAARLTELAKLEPEHVLASAIAVSGALDAEALCWDVGEIVELRGRTAP
                     TQLARPLNLVSSSEADNVPSGAEREVVVEESRGFRA"
     gene            complement(6238..9030)
                     /gene="topA"
                     /locus_tag="VFC01_32775"
     CDS             complement(6238..9030)
                     /gene="topA"
                     /locus_tag="VFC01_32775"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HZN84430.1"
                     /translation="MADGDRGSGRTGAVRRLVIVESPTKARKIAGYLGSNYIVESSRG
                     HIRDLPRAAADVPAKFKSEPWARLGVNVDEDFEPLYIVSPDKKGTVTELKGLLKDVDE
                     LYLATDGDREGEAIAWHLLETLKPRIPVKRMVFHEITEPAILAAAEDPRDLDNDLVDA
                     QETRRILDRLYGYEVSPVLWKKVAPKLSAGRVQSVATRIIVQRERERMAFRSAGYWDV
                     TAELDASVSNSEATPPTFTAKLNSVDGRRVATGRDFDSLGELKKPDEVLVLDEARAGA
                     LASGLRGAQLAVSSVEQKPYTRRPYAPFMTSTLQQEAGRKLRFSSDRTMSIAQRLYEN
                     GYITYMRTDSTTLSQSAINAARNQARQLYGEEYLHPSPRQYTRKVKNAQEAHEAIRPA
                     GDVFQTPGQLHSQLDTDEFRLYELIWQRTVASQMADARGTTLSLRISGQSSDGQQVVF
                     SASGRTITFAGFLKAYVESIDEQAGGEADDAESRLPNLEQGQRVDAKELTADGHTTSP
                     PARYTEASLIKALEDLGIGRPSTYSSIIKTIQDRGYVHKKGSALVPSWVAFAVTGLLE
                     QHFSRLVDYGFTAAMEDELDEIASGNEQRTTWLSNFYFGGEHGVPESIARSGGLKKLV
                     GVNLEGIDAREVNSIKLFDDEEGRPVYVRVGKNGPYLERMVAGDDGEPKPQRANLKDE
                     LTPDELTLDLAEKAFATPQEGRSLGVDPETGHEIVAKDGRYGPYVTEVLPEPESDDDD
                     GAGSGAKKPKKPTGPKPRTGSLLRSMSLDTVTLDDALRLLSLPRVVGVDPNTSEEITA
                     QNGRYGPYLKRGTDSRSLATEEQMFDITLDEALKIYSEPKRRAGQRASTPPLRELGND
                     PATDKPMVIKDGRFGPYVTDGETNASLRKGDDVLSITDERAAELLADRRARGPVKRTK
                     KAAKKKAPAKKAAKKS"
     gene            complement(9165..>9279)
                     /locus_tag="VFC01_32780"
     CDS             complement(9165..>9279)
                     /locus_tag="VFC01_32780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005072604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84431.1"
                     /translation="GAHPALRISGRRRLSRLVETVGEPPGDVEAFSLWPRM"
BASE COUNT         1602 a         3161 c         2871 g         1645 t
ORIGIN      
        1 gaagaagtca ccggaagagg tcggcaagga agactccgag gcgctgcaga acgggcagct
       61 gacgcccaag cagcatcagc gtttggtcga gaacaccacc ctgagccgcg aacagcagac
      121 ggccctcgac tgacactccc gccggagcaa atgtcatatt tggagggctt ctcgcgtgcg
      181 ttcggcgaca gacgccggcg gagatcaaag ccatcatgga tgaggccggc ccggatggtg
      241 gtcgagtcgc cgacgtattt caactcgcct ccaatccgaa catcaagacc gggctgccag
      301 agactgagcc gccgtcgacc agtaatccgg cgagcggcgg caagtacgca tttgccggac
      361 ggcatccaaa aggttctcga tggacccgtc ttgacgccac taagcttcgg cgaaccgacg
      421 cacaaccctg acggctccat cggtcgacct ccactgacga ccgcctctca gccgacggct
      481 ggcctcaacg atctggctga catcatccac cgaggaaacc gagacctgca ggtaggcagc
      541 ggtcttgatt ccggcctctt cgaaaaaacg ccagcagctg ctcgaacaat ccaatggctg
      601 gccggttccg ggcaatgatc cgatgtccga tcgcccgcgc tggtatcacc aattggttga
      661 tccgacgttg cagaacatgt tcaacgcggt caacaaggac gacctggtga tccacgacgc
      721 agtcaccggc tctggtggtg aacggttctt gaacaacctg acccaacatc aatggcaaga
      781 tgacgggctg gcggcaggcg gactattcga ttgggtgggc gaatccgcgg ctcacgattc
      841 gacggggcgc gcggccgaaa ccgcccatgc tttggaggag tacacgagca acaagcacaa
      901 ccagctgttg aatcttcctg atacacaagg tcagtcgctc ggacaggtta atccggagct
      961 gacgcgcgac tgggcgaggg ccttctcccc ctacatggac gacatggtcg gcatgaatac
     1021 cggtgacagc aacgggctat tcgctccgct cgatcccgac agctcggtag aacccgcgaa
     1081 tacgcgacac ctcatgagtg ttttgtattc ggatcacccg ccgttagacg gaggcgccga
     1141 cgccggcgct gcaaagaccg caagtcagat actctccgag agctcgcaag ctcacatcaa
     1201 cagttatctg accgctgctg cagagtcagt cgcggaccgg aacgcgggcg aggataactt
     1261 tgcgatgcag tcggccggta agctccaagc cgcaatggac ttaggtgctt acgatgagcg
     1321 tttcgacacg acgcacgaca aattcgaggc gcagaagcaa gcgcatgaga tgcaatccaa
     1381 actgttcgac ctcggcaagg acgtcatcgg cgagtttcct ggcgggtcaa cggtcggcgg
     1441 cttaggcggg ttgatgaagg acgtcattgt cggctcagat ccagtgatgc ctggtgtgca
     1501 gagcgtggcg ggtcgcgaca tgttcccagt gcagctgcac atggcgcaga cactcgcgca
     1561 ggcaggcgtg ggcgaccccc atttcctcga agtcgttcga ggtcatcttg atgcgaacgg
     1621 acacttcaac attccgcggg aggacggaac accggactac cgatatttca agaacgacat
     1681 caacaactat ctaggctcgg tgggtcaacc gggtgtcatc aacgacatga cccagaacta
     1741 ctggaacacc tacacgcaag cgattatcaa cgcagcaccg ccgcaacagc catgacgcgc
     1801 cgtatcttaa cgatcattgc ggtgatcgcg gccactctgt ccagcgggtg cgacaccaat
     1861 acgacgaaat cgccttccac agcatccaac tcacccgcgc agctgtatcc gaactggcca
     1921 ccactgctca acgacttccg gtttcactgg tccgcggcac cggggatcga tatcaccgag
     1981 ggcccggcga tggtcgtccg tgcgtacctg gagtcttaca acacagcgtg gttcaccctg
     2041 aaccttgaca acctgttccc gggtttccgc agggtcactc ccgaaaatca ggagccagag
     2101 ggcgatttcc tgtggcaact agtccagata cggccgctgg gaagcggata caccaagacg
     2161 gcgaaggatg cgcgcccaca tttcggctac caagcgcttc attttctgga gcttacgcct
     2221 atcgagaatg gtttccgcgc catcgtgtgc agcggcgact tcgcgcattt cgtcgaaagt
     2281 actgcgcggc cgggaaaatt cgtctccatt ggcgacaacg aggagactgc tcaaccggat
     2341 ggagagggtt acagcggcgt ctatcccacc caaattgagc tcacccagcg tgacccacgc
     2401 attgcgccga atccgccccc tcagcgaggt ccggcgccag cgcccgttca ggacgtgttc
     2461 ggcgattggt acttcacggg agcaagctcg tcgtactggg gaccttcgag tgaccgcacg
     2521 gctcgtgact ttccgccgcc cgaactcaaa cagcgatgcg acgtcgcgat gccgacgccc
     2581 gcagccgaac gcatcgcgat gatgaccggc ttcaaggacc agccgccacc acacggcgaa
     2641 gctattcctg gttggcccgc gaaagtccag tgagccagtc ctggtcgtca tgccaatctg
     2701 gatggcatga cagatgtgcg tgaacaaggt ctacaggtct tccgccagct gatgccggga
     2761 gcggaggtca acctgcgcga cggcaagttc ggcgacgagc tactcgagat cggcatcgac
     2821 aacgtgttcg gccgcttatg ggcacgtgac ggactgagcc gtcgcgaccg cagccttgtc
     2881 acgctcggca tcctcatcgc cctgcgcgcc accgacgaac tcaaggccca cgtccagatc
     2941 gcccgcaaca acgggctcac cgaagacgag atcgccgagg tcatctacca cgccagcggc
     3001 tacgccgggt tccccgccgc cgacaccgcg aacaagatcg cccgcgatgt gtttgacgaa
     3061 acatcgtgac ggatttcctt ctgcggagcg tcaacatctg atgacgacct ggacaagcac
     3121 acgtcctccg agaagcgcaa agccgagcca cctaggagaa tcgaactcct gacctattca
     3181 ttacgagtga atcgctctac cgactgagct aaggtggcgc gcccgggctt gccgggcggg
     3241 acgagtctac ggcaggcccc accggcagcc caagtcagtt gcgcagcgcc cgcccgaccg
     3301 tggccaccat cgcatcgatc gcgaacttcg gcttcacatt caccgccagc gcctcgcggc
     3361 actccagcac cgcctcgata caccgcagca gcttctccgg cgacgcatgc gcggccatcg
     3421 cggcaacccg gtccgtcatg tccggatggt tcgcctggac cgagtccgcg ccagacgaaa
     3481 ccagcagcgc gtcgcgaaag tacgtcgcga gatcgatcag ggcccgatcc agcgcgtccc
     3541 gcgacgcccg cgtctgccgc gacttctgcc gtcgctcaag gtctttgatc gcgcccgtcg
     3601 caccgcgcag cgttccggcg gtgcccttgc cggtgccgcc cgcacccaat gccgtacgca
     3661 actcctcggt ttcggcctcg ttgcggtctt cggtcagcgc ccgcgcttct gcctcggccg
     3721 tcgccaccaa ctcctcggcc gcggcgtacg cccgcgatgg cgttgccgcg tcacgcgcca
     3781 accccaacgc ccgctgcctg cggtcgcgcg ccagctcatc ggtcgccagc cgccgcgccc
     3841 gcccgacgtg gcccccactg accgacgccg cccacgaagc ctcgtcctcc ggcaacccgt
     3901 ccgtctcgat cagcacccgc gcaatcgcgt cgaccggtgg cgtgaccagc gctacatgcc
     3961 ggcaccgcga acgcagcgtg atcgcgatgt cctcgggatc caccgacggc gcgcacagca
     4021 gaaaaaccgt cgacggcggc ggctcctcga ccaccttgag cagcgcattc gccgcccctt
     4081 cggtcagccg atcggcatcc tcgatcagca cgatctgcca ccgcccagtc cccggccgcc
     4141 gcgacgcgat ctgcacgatg ttgcgcatct cgtccacgcc gatcgacaac ccctcgggga
     4201 tgatccggcg cacatcggcg tgggtcccgg ccatcgtcgt cgtgcaggcc cggcattgcc
     4261 cgcaaccggg gacgccgtcg gaggtgcatt gcagcgccgc agcgaagcac agcgccgcga
     4321 tcgagcgccc cgaccccggg ggcccggtga tcaaccacgc gtgtgtcatg gtcccggtcg
     4381 cggcgccatt gtgagccgaa tcaccgcgcg cggcctgcgc cgctgcaacc aattcggctt
     4441 ccacggcgtc ttgacctact agacgcgaga acacaccggc catcgagata acagtaatgc
     4501 tgcgaaccga cacgtccgtc ccacagcgtc attttccgtc cgcccggccc gatacggttg
     4561 acctgtgacc accgaggcca ttccgatcgg gcgaatcagc gcattcgtcc gttggtttgt
     4621 gcgcacgccg tggccggtgt tcacgctcgg catgctgcag gccgacatca tcggcgcgct
     4681 gctcgtgctg ggctttctgc gcttcgggct gcctcccgag gaccgcatcc agctacagga
     4741 tctgcccgcc ttcaacttgg cgatcttcct gggttacctg ttcctgtcgt tcaccatcgg
     4801 ctcgtatctg accctgcgga tgctcatccc agtgatgcgc tggcagcgcc gcgacacgct
     4861 gctcggcgcg cgggacccgg ccgccaccga aatggcccgc acgcgagcgc tgaagatgcc
     4921 gttctaccgc tcggtgatca acgtgacgaa ctggctgctg ggctcgatcg tgttcatcgt
     4981 cgccagctgg ccggtagcca gcaaatccgc gccggtggtg gcggtcgcca ccgggctggg
     5041 cgccaccgcg acggcgatca tcggctatct gcagtccgaa cgggtgctgc ggccggtggc
     5101 cgtggccgcg ctgcgcggcg gcgtaccgga gaacttccgc gcgccgggcg tcattcagcg
     5161 ccaggtgctg acgtgggtgc tgtccaccgc cgtgcctatc ctcgcgatcg tgctggcggt
     5221 ggtcgccagc aaggtcgaga ttctcaccgc gcccgccgac cgtctcacca cgccgatcct
     5281 tctgctggcc atcgccgcac tggtgatcgg cttgttcggc accgtgctgg tcgccatgtc
     5341 gatcgccgac ccgctgcgcc aattacgttg ggccctgggc gaagtgcagc gcggtaacta
     5401 caacgctcac atgcagatct acgacgccag cgaattgggt ttgctgcagg ccggattcaa
     5461 cgacatggtg cgcgacctcg ccgagcggca acggcttcgc gacctgttcg gccgttacgt
     5521 cggcgaagat gtggcccgtc gcgcgctgga gcgcggcacc gaactcggtg gccaggaacg
     5581 cgacgtcgca gtgcttttcg tcgatctggt cgggtcgacg cacctggcgt cgacgaggcc
     5641 tgcggccgag gtcgtgaacc tgctcaacga gttcttccgc gtcgtcgtcg acaccgtgaa
     5701 ccgccacggc gggttcgtca acaagttcca gggcgacgcg gcgctggcca tcttcggcgc
     5761 gccgatcgac caccccgacg cctcgggcgc ggcgctggcc gccgcccgcg agctgcacga
     5821 cgaactggtg aatgtgttgg gccagaccga attcggtatc ggcgtgtccg cgggacgggc
     5881 gatcgccggt cacatcggcg cccaggcccg cttcgagtac accgtgatcg gcgatccggt
     5941 gaacgaggcc gcccgcctca ccgagctcgc caagctggag cccgagcacg tgctggcgtc
     6001 ggcgatcgct gtcagcggtg cgctcgacgc cgaggccttg tgctgggacg tcggcgagat
     6061 cgtcgagctg cgcggccgca ctgctccgac tcagttggcc cgtccgctga acctggtatc
     6121 gagcagcgag gccgacaacg tcccgagcgg cgccgaacgt gaagttgtcg tcgaagaatc
     6181 gcgcggattt cgcgcataag ctcacgctcg gcgcgcaagg cccgctgagc aacggcgcta
     6241 gctcttcttt gcggccttct tggccggcgc ctttttcttg gccgctttct tcgtccgctt
     6301 gacggggcca cgcgcacggc ggtcggccag cagttcggcg gcgcgctcgt cggtgatcga
     6361 cagcacgtcg tcgcccttgc gcaggctggc gttggtctca ccgtcggtga cgtacggccc
     6421 gaaccggccg tccttgatca ccatcggctt gtcggtcgcc gggtcgttgc ccagctcgcg
     6481 cagcggcggg gtcgacgccc gttgtcccgc acgccgtttc ggctccgagt agatcttcag
     6541 cgcctcgtcg agcgtgatgt cgaacatttg ctcctcggtc gccaacgagc gagaatcggt
     6601 gccgcgcttc agatatgggc cgtagcggcc gttctgcgcg gtgatttcct cactggtgtt
     6661 gggatcgacg ccgacgaccc ggggcagcga cagcaatcgc agcgcgtcgt cgagcgtcac
     6721 agtgtcgagg ctcatcgacc gcagcagtga gccggtgcgc ggcttgggac ccgtcggctt
     6781 cttgggcttc ttcgccccgc tgcctgcgcc gtcgtcgtca tcagactcgg gctcgggcag
     6841 cacctcggtg acgtacgggc cgtagcggcc gtccttggcg acgatctcgt gaccggtctc
     6901 gggatcgacg cccagcgatc gcccctcttg cggtgtggcg aaggcctttt cggcaagatc
     6961 gagcgttagc tcgtcgggtg tgagctcatc cttgagattg gcccgctgcg gcttgggctc
     7021 gccgtcatcc ccggcgacca tccgctccag gtacggcccg ttcttaccaa cgcgcacata
     7081 gacgggtcgg ccctcttcat cgtcaaacag cttgatggag ttgacttctc gggcgtcgat
     7141 gccctcaagg ttcacgccga caagcttctt cagcccgcct gaacgcgcga tcgactcggg
     7201 cacaccgtgt tcgccgccga aatagaagtt gctcagccac gtggtgcgtt gctcgttgcc
     7261 cgacgcgatc tcgtcgagct cgtcctccat cgccgcggtg aagccgtaat cgaccaatcg
     7321 gctgaaatgc tgctcaagca ggccggtgac cgcgaacgcc acccacgacg ggaccagcgc
     7381 gctgcccttc ttgtggacat aaccgcggtc ctggatggtc ttgatgatcg acgagtacgt
     7441 cgacggccga ccgatgccca gatcttccag cgccttgatc agcgacgcct cggtgtagcg
     7501 cgccggcggc gacgtggtgt gcccgtcggc ggtgagttcc ttggcgtcga cccgctggcc
     7561 ctgctcgagg ttcggcagcc ggctctcggc gtcgtcggcc tcaccgccgg cctgctcgtc
     7621 gatgctctcg acgtaggcct tcaggaagcc agcgaacgtg atggtgcggc cgctcgcgga
     7681 aaacaccacc tgctgcccat cgctggactg cccggagatg cgcagcgaca gcgtcgtgcc
     7741 cctggcgtcg gccatctgcg aggcgacggt gcgctgccag atcagctcgt agagccggaa
     7801 ctcgtcggtg tccagctggc tgtgcagctg gcccggcgtc tggaacacgt cgcctgcggg
     7861 ccggatggcc tcgtgcgcct cttgggcgtt cttgaccttg cgggtgtact gccgcggcga
     7921 cggatgcagg tactcctcgc cgtacagctg gcgggcctga ttgcgcgccg cgttgatcgc
     7981 cgactgcgac agtgtcgtcg agtcggtacg catataagtg atgtagccgt tctcgtagag
     8041 ccgctgggcg atgctcatcg tgcggtccga ggagaaccgc agcttgcggc ccgcctcctg
     8101 ctgcagcgtc gacgtcatga acggcgcata cggtctgcgg gtgtacggct tctgctcgac
     8161 cgacgacacc gccagctgcg cgccgcgcag cccggaagcc agcgcacccg ctctggcctc
     8221 atcgagcacc agcacctcgt cgggcttctt gagctcgccc agggaatcga agtcgcgtcc
     8281 ggtggccacc cgccggccgt cgaccgagtt cagcttggcc gtgaacgtgg gcggcgtcgc
     8341 ctccgagttg gagacgctgg cgtcgagttc ggcggtgacg tcccaatagc cggcgctgcg
     8401 gaacgccatc cgctcgcgct cgcgctggac gatgatgcgg gtcgcgacgg attgcacgcg
     8461 cccggccgac agcttcggcg cgaccttctt ccacagcacg ggactgacct cgtagccgta
     8521 cagccggtcc aagatgcggc gggtctcctg cgcgtcgacc agatcgttgt ccagatcccg
     8581 cgggtcttcg gcggcggcca ggatggccgg ctcggtgatc tcgtggaaca ccatccgctt
     8641 gaccgggatg cgcggcttca gcgtctccag caggtgccag gcgatggcct cgccctcccg
     8701 gtcgccgtcc gtggccagat agagctcgtc gacatccttg agcaggccct tcagctcggt
     8761 gacggtgccc ttcttgtcgg ggctgacgat gtagagcggc tcgaagtcct cgtcgacgtt
     8821 gacgccgagc cgtgcccacg gctccgattt gaacttggcg ggcacgtcgg cggccgccct
     8881 gggcagatcc cgaatgtgac cacgggagga ctcgacgatg taattggagc ccagataacc
     8941 ggcaattttg cgtgctttgg taggcgactc gactatgacg agtcgccgca ctgcaccggt
     9001 gcggccgctg ccgcggtccc cgtcagccaa cttgccttcg ctccactttc tatgttgccg
     9061 tccgccgccg gattcatgcg ttcttcacta tccgtcgccg ttggcaactg acaatttcgc
     9121 accccggacg tgccgacgca aactgaccct ccggcgccag acccctacat ccgtggccag
     9181 agcgagaacg cctctacgtc gcccggaggt tccccgacgg tctctaccag gcgcgatagg
     9241 cgtcgccggc cactgatccg cagcgccgga tgagctccc
//
LOCUS       DASRJA010000725         3801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3726961,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000725 DASRJA010000000
VERSION     DASRJA010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3726961"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFC01_32785"
     CDS             complement(<1..648)
                     /locus_tag="VFC01_32785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84432.1"
                     /translation="MNSVLTVVGQRDPKLEGLVRASGRVTTVIWLADLNALLDPKASQ
                     PDLVLIDIRRLSAVPSQLATLKRQHPATNVILLTSTLDPAVMLEAMRAGVNECIAEPL
                     QQDDIDAAIARLVGLQTKAVAGPIFAFVGAKGGVGTTTTAVNVATTLTKLSSAGALLI
                     DLHLAYGDAAVFLGADARFSLLDALENLHRLDAQFLKSLVSRTVSGLDLLASADRP"
     gene            complement(660..1970)
                     /locus_tag="VFC01_32790"
     CDS             complement(660..1970)
                     /locus_tag="VFC01_32790"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HZN84433.1"
                     /translation="MTRHAGSSSRLGDQRGAVLIQVAVAMLALLALASFVFDYGVMWV
                     SRGQAQNAADAGALSGAISLAFNSPTDQAAARARAIAMAQANNVWNQAPSVTPADVTF
                     PACPPGIPGPQDQCVRVNVFRNQARGNPLPTFFAKLSGVPNQGAVATATAQVVTGDTT
                     ACLRPWAVVDRWNEFGPEGPAPLPTSTYDKYSTGQGNTPPQENDVYIPPSLSGPGTGY
                     SLPADRGRQFGIKMGPSGGNEISSGWFRTLDLPRADTTQLGNNTVQTNILTCNGMPAS
                     FANPATVCPASIPNNWTDTAYWAARGCYRVQTGATVGSTRNSIEALMAQDPSARWSNG
                     DITGSNWDPPSSSPRVVPVGVMDIDWYLSHDPTGNNGVLRMVNIFGFFIEGMGDVDRN
                     TGAITLAANGQSVIGRVMTIPATAVGNSTLPGNASFLVTVRLVR"
     gene            complement(1967..3424)
                     /locus_tag="VFC01_32795"
     CDS             complement(1967..3424)
                     /locus_tag="VFC01_32795"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HZN84434.1"
                     /translation="MSTLIKQIRPARAGVVAVTLILAGQCQLPMSAQQASVAPPAPPA
                     TAPAQATAVTPARPQFDRVLVTAGRSTVVPTDFDVTRIAVTDPAVADAVVVQPREVLV
                     DGKKPGTVSLILWSSTQRKQYDIVVEPAITGLEQQLQALFPGEDISVGVSEEAIVLSG
                     HVSSTSIMLRAAEIATASASKSKIINLLQVPGGSESQQVMLQVRFAEVNRRALTELGL
                     NLFVNRERWAARSTTQQFSAPNFDDEKPGGVVFSDFLNLMFFDKKEGIGGVMRALQQR
                     GGFQSLAEPNLIAYNGQEASFLAGGEFPVPVVQAGSTNAVTIVFKEFGIRLNFKPTIA
                     GDLIRLKVRPEVSTLDFANGVTLQGFRIPALTSRRAETDVELRDGQSFAIAGLLDNIR
                     QDDKAAIPILSQIPVLGNIFKSKAERAEETELMVLITPRLIRALNPDEVPPLPTSIRP
                     FIKKGDIGQQLDGAGGVVDAPRKSKDASPAVRREQ"
     gene            complement(3421..>3801)
                     /locus_tag="VFC01_32800"
     CDS             complement(3421..>3801)
                     /locus_tag="VFC01_32800"
                     /inference="COORDINATES: protein motif:HMM:NF028286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HZN84435.1"
                     /translation="VVVSNVQVLTAGTRYDQEKAKDGAPIPSTVVTLLVKPEDGERVA
                     LAASAGQIMLTLRNPLDQDQTATAGVRTTGLFGQAAPAPVKTPTVHRGPAKPPAPVVA
                     TNPTPAAYTVEAFRGAKRTQEIVR"
BASE COUNT          662 a         1206 c         1207 g          726 t
ORIGIN      
        1 tggtcgatcc gccgacgcca gcagatcgag tcctgaaacc gtgcgggaga cgaggctctt
       61 caggaattgc gcgtccagcc gatgcaggtt ttcgagcgca tcgagtagcg agaatcgcgc
      121 atcagcgcca aggaagaccg cggcgtcgcc gtacgccagg tgcagatcga tgagtaacgc
      181 accggccgac gaaagcttcg tgagcgtcgt cgcgacgttc actgcggtgg ttgtcgtccc
      241 gaccccgccc ttggcgccga cgaacgcgaa gatcggtccc gcgactgcct tggtctgcag
      301 tccgacgagg cgagcgatcg ccgcatcgat atcgtcctgt tgcagcggtt ccgcgatgca
      361 ctcgttcacc cccgcgcgca tggcttcgag catcacggcc gggtcgagcg ttgacgtcag
      421 caggatgacg ttcgttgccg gatgctggcg cttgagcgtg gcgagctgcg acggaaccgc
      481 cgacaggcgg cggatgtcga tcagcaccag gtctggttgt gaagccttcg ggtcgagaag
      541 cgcattcagg tcggccagcc agatgaccgt cgtcacccgg ccggatgcgc gaaccagccc
      601 ctcgagcttc ggatcgcgct gaccaacaac tgtcaataca ctattcacgg tgtcctctct
      661 taacgaacga gtctgacggt cacgagaaac gaggcgtttc ccggcagtgt ggaattgcca
      721 acggcagtcg ccggaatcgt catgacccgg ccgatgaccg actggccatt ggctgccagt
      781 gtgatcgcgc ccgtgtttcg atcgacgtcg cccatgcctt cgatgaagaa gccgaagatg
      841 ttgaccatcc gcagaacgcc gttgttgccg gtcgggtcgt ggctgagata ccagtcgata
      901 tccatcacgc caaccggaac gacccttgga ctactcgaag gcgggtccca gttgctcccc
      961 gtgatgtcgc cgttcgacca tcgcgcactc gggtcctggg ccatcagcgc ttcgatcgag
     1021 ttgcgtgtcg aaccgacggt cgcgccggtc tgcacgcgat aacatccgcg ggcggcccag
     1081 tacgcggtgt cggtccagtt attgggaatg gacgccgggc aaacggtcgc cggattcgca
     1141 aagctggcgg gcattccgtt gcaggtcaga atgttggtct ggaccgtgtt gttgcccaac
     1201 tgcgtggtgt cggcgcgagg cagatcgagc gtcctgaacc agcccgacga aatctcgttt
     1261 ccgcccgatg gtcccatctt gattccgaat tgccggccgc ggtccgccgg cagcgagtag
     1321 cccgtgccgg gaccggataa cgacggtgga atgtagacgt cgttctcctg cggaggagtg
     1381 tttccctggc cggtcgagta cttgtcgtag gtcgaggtcg ggagtggtgc tggtccctcg
     1441 ggtccgaatt cgttccatcg atcgacgaca gcccacggcc tcaggcatgc ggtcgtatcg
     1501 ccggtcacaa cttgcgcggt cgcggtggcg accgctccct gattcggaac accactcagc
     1561 ttcgcaaaga atgtcggcaa tggattgccg cgagcctggt tgcgaaacac gttcactctc
     1621 acacactggt cctgcggtcc cggtattccc ggtgggcacg cagggaacgt cacgtccgca
     1681 ggcgtgacac tgggcgcctg gttccacacg ttgttggcct gagccattgc gatggcccga
     1741 gcccgcgccg ccgcttgatc tgtcgggctg ttgaacgcga gtgagattgc gccggacaat
     1801 gcccccgcat ccgcagcgtt ctgcgcctga ccgcggctga cccacatcac cccgtagtcg
     1861 aagacaaagg aggcgagtgc gagcagggcg agcatcgcca ctgcaacctg aatcagcacc
     1921 gcaccacgct ggtcgccgag acgcgatgaa gagccggcgt gtctggtcac tgctccctcc
     1981 tcaccgcagg tgatgcgtct ttgctcttgc gcggtgcatc cacgacgccg ccggcgccgt
     2041 cgagttgctg accgatgtcg cccttcttga taaagggccg aatgctggtc ggcagcggcg
     2101 gtacttcatc cggattcaat gcgcggatga gacgtggcgt gatgagcacc atcaactcgg
     2161 tctcttccgc ccgctcggcc ttgctcttga aaatgtttcc gagcaccggg atctggctca
     2221 gaatcggaat cgcggccttg tcgtcctgac ggatgttgtc gagcaggccg gcaatcgcaa
     2281 atgactggcc gtcccgcagc tcgacgtccg tctccgcgcg tcggctcgtg agcgccggga
     2341 tgcggaagcc ctggagcgtc acgccattcg cgaagtcgag ggtgctgacc tcgggccgta
     2401 ccttaagtcg gataaggtct ccggcaatcg tcggtttgaa gttcaatcga atgccgaact
     2461 ctttgaagac aatcgtgact gcgttagtgc ttcccgcctg caccaccggc acaggaaatt
     2521 caccgcccgc gaggaagctc gcctcctggc cgttgtaggc aatcaggttc ggctctgcga
     2581 gactctgaaa gccaccacgc tgttgaagcg ctcgcatcac gccgccaatg ccttccttct
     2641 tatcgaagaa catcaggttg agaaaatcgc tgaacacgac accacccggc ttttcgtcgt
     2701 cgaagttcgg tgcggagaac tgctgcgtgg tcgaccgtgc ggcccaccgc tcgcggttga
     2761 cgaaaaggtt taagccgagc tcggtgaggg cgcggcggtt gacctcggcg aagcgcacct
     2821 ggagcatcac ctgctggctc tcggagccgc ctggcacctg cagcaagttg atgatcttgc
     2881 tcttcgaggc tgacgcggtt gcgatctcgg cagcccgcag catgatgctc gtgctcgaga
     2941 catggccaga cagcacaatc gcttcctcgc tgacaccgac cgaaatgtct tcaccgggga
     3001 acagcgcctg cagctgctgt tcgagcccgg tgatcgccgg ctcgacgacg atgtcgtatt
     3061 gcttgcgctg cgtcgatgac cagaggatca ggctcaccgt ccccggcttt tttccatcga
     3121 ccagcacctc gcgcggctgc acgaccaccg catcggcgac agccgggtcg gtgaccgcga
     3181 tgcgcgtcac atcgaaatct gtcggcacga ccgtggaccg gccggcggtc acgagaacgc
     3241 gatcgaactg cggacgtgcc ggagtcacgg cggtggcctg tgccggcgcc gttgccggcg
     3301 gtgccggcgg cgcgactgac gcctgctgtg ccgacatcgg cagctggcac tgcccggcca
     3361 gaatcagcgt gactgccacc acacccgcgc gtgccgggcg aatctgcttt atcaaagtgc
     3421 tcatcgaaca atctcctggg ttcgcttggc gcctcggaac gcctcaacgg tgtaagcggc
     3481 cggcgttgga ttcgtggcaa caactggtgc cggcggtttc gccggaccgc ggtgcacagt
     3541 cggcgtcttc actggcgccg gtgccgcctg gccgaacaga cccgtcgtcc gcacgccggc
     3601 ggtcgcggtc tgatcctgat cgagggggtt tcgaagcgtc agcatgatct ggccggcgga
     3661 cgcggcgagc gcgacgcgtt cgccatcttc gggtttcacg agcaaggtca ccaccgtcga
     3721 cggaatcggt gcgccgtcct tcgccttctc ctggtcgtag cgggtgccgg ccgtgagcac
     3781 ttgaacattg ctgacgacga c
//
LOCUS       DASRJA010000726         7911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2380682,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000726 DASRJA010000000
VERSION     DASRJA010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2380682"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFC01_32805"
     CDS             <1..484
                     /locus_tag="VFC01_32805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN84436.1"
                     /translation="SGAGKTTLLMAMAQAPKNPLQAAFFAEDAHLFATTVRDNLLVAR
                     GDATDDELRNALRRVGLGEWLDGLRQGLSTVLVGGASAVSAGQRRRLLLARALISTVP
                     TVLLDEPTEHLDAADGSRLLTELLVPGALFPADRTVVVATHHLPDDIVCPVFNLRTGE
                     "
     gene            495..974
                     /locus_tag="VFC01_32810"
     CDS             495..974
                     /locus_tag="VFC01_32810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HZN84437.1"
                     /translation="MSEPPPPPQYGPYPGSYPPPPPQPYAGYAPAPTGPKNGLGIAAL
                     VVAIIGLVFCWTVVGGVILGFTAVLIGFVARGRVKRGQATNGGVAIAGIVLGFLAIIV
                     SLIFIPIWYSVFEEVGGTDYVDCLNKAGNDTDAIQKCADQFRDRVENQFSVSVTPHS"
     gene            complement(971..2356)
                     /locus_tag="VFC01_32815"
     CDS             complement(971..2356)
                     /locus_tag="VFC01_32815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HZN84438.1"
                     /translation="MSESHVNFAPRLRVVVHVDSPAARWLGALAVLSLFCWLILLLAR
                     EHDRDWQAAGRLAWSLTLLSAVALIARGIFLGRPVTAAHAWLALAALLAGLCAHVLVF
                     DFLGNLVIVSAGGLLMWPTAVRPNPADLPRIWALVERTKGDPLAPFAMQDLKCYHFSA
                     DGTAAIAYRTRLGYAVVSGDPIGDDTRFAELVADFAAMCHSRGWRVVVLGCSQQRLEL
                     WADATVLGQSLRAVPIGRDVVIDVATFEMVGRKYRNLRQAVQRTQNFGVTTEVVSEQG
                     LDEPRLAELTEVLLASPKGARIERGFSMCLDGALEGRYPGVQLIVARDKDGRVQGFHR
                     YATAGQGSEVSLDVPWRRRGAPNGIDERLSVDMIAWARDNGAQRLSLAFAAFPEIFND
                     KNRGKISSLIYTAIHLGDALIALESLYRYLRKFHALGDRRYVMASLTQIVPLLVVLLS
                     LEFLPRRRRLT"
     gene            complement(2376..3245)
                     /locus_tag="VFC01_32820"
     CDS             complement(2376..3245)
                     /locus_tag="VFC01_32820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HZN84439.1"
                     /translation="MSTADFEELLAVLDLKQVADDTFIGSHPSKNPVRTFGGQMVAQS
                     FVAGSRTVSEKLPPSSISAHFIAGGDPEKDLEFHVVRLRDERRFANRRVDVMQDGQLL
                     TTAMISYLSGGRGIEHGVEPPELPAPESVPPVDELLRGYEDVVPHFVNALRPIDWRYT
                     NDPTWVMRDKGEKLTYNRVWMKAEGAMPDDPVLNCAALVYSSDTTVLDSIITTHGLSW
                     GYDRIFAVTTNHSVWFHRPVRFDEWVLYSTSSPVAADSRGLGTGHFFDRSGQLLATVV
                     QEGIVKYFPGASR"
     gene            complement(3270..4688)
                     /gene="pyk"
                     /locus_tag="VFC01_32825"
     CDS             complement(3270..4688)
                     /gene="pyk"
                     /locus_tag="VFC01_32825"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZN84440.1"
                     /translation="MNRRGKIVCTLGPATSTDETVRALVEAGMDVARLNFSHGDYADH
                     EANYKRVRAASDLTGHAVGILADLQGPKIRLGRFKDGPTFWANDETVRITVDDCEGTH
                     DRVSTTYKRLAEDATPGDRVLVDDGNVGLIVESIDGTDVVCKVTEGGAVSNNKGMSLP
                     GMSVSAPALSEKDIQDLEFALQVGVDLIALSFVRSPADIELVHEIMDRVGRRVPVIAK
                     LEKPDAIDNLEAIVLAFDAIMVARGDLGVELPLEEVPLVQKRAIQMARENAKPVIVAT
                     QMLESMIENSRPTRAEASDVANAVLDGADAVMLSGETSVGKFPFEAVKTMARIIHAVE
                     ENSVAAPPLAHVPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVRRLARLHTPLPV
                     LAFTSLPEVRSQLALTWGTETFIVPHIASTDGMIRQVDKSLLELGRYKRGELVVIVAG
                     APPGTVGSTNLIHVHRIGEEDV"
     gene            complement(4716..6224)
                     /locus_tag="VFC01_32830"
     CDS             complement(4716..6224)
                     /locus_tag="VFC01_32830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HZN84441.1"
                     /translation="MADPTGFLKIPKVEAAKRPVDERVGDWREVYERQDPHQRAGEVS
                     QQAGRCMDCGIPFCHSGTAGCPLGNLIPEWNDLVRRGRWDAASDRLHATNNFPEFTGR
                     LCPAPCEAACVLSIAEEQTGGSVTIKRIEQTIVDQAWMDGIVEPQPAAISTGKKVAVV
                     GSGPAGLAAAQQLTRAGHDVTVYERDDRIGGLMRYGIPEYKLEKRTLNQRLAQMRAEG
                     TRFVTETEVGVDLPVERLRKQYDAVVLAVGALRARDNEVEGRHLKGVHLAMEHLVPAN
                     KECEGDGASPITAKDKHVVIIGGGDTGADCLGTAHRQGATSVTQLDYNPEPPEYRDES
                     RSPWPMWPIVLRTRLSPAHAEGGARHYEVAVQRFIGDERGQLRAMEIAEVKVERDAGG
                     RRRITPVGDSLEIPCELALLAIGFDGVEHMPLLDGLGLTLNRRGALPCGSDWQTDAPG
                     VFVCGDAHRGASLIVWAIAEGRSAAYAVDAYLMGESDLPEPVKPGALPLAVV"
     gene            complement(6217..>7911)
                     /locus_tag="VFC01_32835"
     CDS             complement(6217..>7911)
                     /locus_tag="VFC01_32835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HZN84442.1"
                     /translation="LVNATDIQIKMAQGAKPGEGGQLPGFKVYPNIAKTRHSTPGVGL
                     ISPPPHHDIYSIEDLAQLIHDLKNANSEARIQVKLVSSVGVGTVAAGVSKAHADVVLI
                     SGHDGGTGAAPLTSLKHAGVPWEIGLADTQQTLVLNGLRDRITVQCDGGMRTARDVIV
                     AALLGAEEYGFATAPLVVSGCIMMRVCHLDTCPVGVATQNPELRARFNGKPEFVENFF
                     RFIAEDVRHYLAELGFRSIDEAVGHAEVLDTVEGVAHWKTQGLDLTPVFTVPRDASQR
                     RRIRDQDHGLDEALDRTLIQLAEGALEDAHPVRLELPVRNVNRTVGTLLGAEVTRRYG
                     AAGLPDDTIHVTLTGSAGQSIGAFLPPGITMELIGDANDYVGKGLSGGRIIVRPPDDV
                     LFLPEDNVIAGNTLLFGATSGEVYLRGRVGERFAARNSGALTVVEGVGDHACEYMTGG
                     RVVVLGKTGRNMAAGMSGGIAFVLGLDPARVNTETVELQRLEPEDLTWLRDVIARHAA
                     YTGSTLATSVLSDWPRRSAQFTKVMPRDYQRVLEATLVAKREGRDVDTAIMEASRG"
BASE COUNT         1281 a         2666 c         2612 g         1352 t
ORIGIN      
        1 cagcggtgcc ggcaagacca cgctgttgat ggcgatggcg caagcgccca agaacccttt
       61 gcaggcggcg tttttcgccg aagacgcgca tctgtttgcc accactgttc gtgacaatct
      121 gctggttgcc cgcggtgacg caaccgacga cgaactgcga aatgcgttgc ggcgggtagg
      181 ccttggcgag tggctcgacg ggttgcggca gggcctgtcg accgtgcttg tcggcggtgc
      241 gtcggccgtg tccgccgggc aacgcaggcg gctgctgctg gcgcgggcgc tgatctcgac
      301 ggtgccgacc gtgctgctcg acgaaccgac cgagcacctc gacgctgccg acggcagccg
      361 gctgctgacc gaactccttg tccccggcgc gcttttcccg gcggatcgca ctgtcgtcgt
      421 cgccacccac cacctgcccg acgacattgt ctgtcccgta ttcaatctca ggaccggcga
      481 gtagcctcac cggcatgagc gaaccgccgc caccacccca atatgggccg tatcccggca
      541 gctacccgcc gccaccgccg cagccctacg ccgggtacgc cccggcgccg accgggccga
      601 agaacggtct cggcatcgca gccctggtgg tcgcgatcat cgggctggtg ttctgctgga
      661 ctgtggtcgg gggcgtcatc ctgggcttta ccgcggtcct catcgggttc gtcgcgcgcg
      721 gtcgcgtcaa acgcggccaa gccaccaacg gaggcgtcgc gatcgccgga atcgtgttgg
      781 ggttcttggc gatcatcgtc tcactcatct tcattccgat ctggtactcg gttttcgaag
      841 aggtcggtgg gacggactac gtcgactgcc tgaacaaggc cggcaacgac acggacgcga
      901 tccagaagtg cgccgaccag ttccgcgacc gtgtcgaaaa ccagttctcg gtctcggtca
      961 caccgcactc ctaggtcagg cggcggcgcc tcggcagaaa ttccagcgac agcagcacga
     1021 caagcagcgg cactatctgc gtcagcgacg ccatcacgta gcggcggtcg ccgagcgcat
     1081 ggaacttgcg caggtagcgg tacaacgatt cgagggcgat cagagcgtcg cccaggtgga
     1141 tcgcggtgta gatcaggctg gagatcttgc cgcgattctt gtcgttgaag atctccggga
     1201 atgccgcgaa cgccaacgac agccgctgtg cgccgttgtc gcgggcccac gcgatcatgt
     1261 cgaccgagag ccgctcgtcg ataccgttgg gcgcgccgcg gcgccgccac ggcacgtcca
     1321 gtgacacctc gctaccctgc ccggcggtcg cataccggtg aaagccctgc acccgtccgt
     1381 ctttatcgcg ggcgacgatc aactgcacgc cgggataccg gccctcgagg gcgccgtcga
     1441 gacacatcga gaacccgcgc tcgatgcgcg cacccttcgg ggacgccagc agcacctccg
     1501 tcagctccgc aagcctcggc tcgtcgaggc cctgctcgga caccacctcg gtggtgacac
     1561 cgaagttctg cgtacgctgc acggcctgac gcaggttgcg gtacttgcgc ccgaccattt
     1621 cgaacgtcgc cacgtcgatg accacgtcgc gcccgatcgg caccgcccgc agcgactggc
     1681 cgagcaccgt cgcatcggcc cacaactcca gccgctgctg gctgcaaccc agcacgacca
     1741 cgcgccagcc gcgcgagtgg cacatcgcgg cgaagtccgc gacaagctcg gcgaagcggg
     1801 tgtcgtcgcc aatcgggtca ccgctgacca ccgcatagcc cagccgggtg cggtaggcga
     1861 tggccgctgt gccgtcggcg ctgaagtgat aacacttcag atcctgcatg gcgaacggcg
     1921 ccagcggatc acccttcgtc ctttccacca gcgcccaaat ccgtggcagg tcagcgggat
     1981 tcggccgcac ggcggtcggc cacatcaaga gcccaccggc cgacacgatg accagattgc
     2041 cgagaaagtc gaacaccagc acatgcgcgc agaggccggc cagcagcgcc gccagcgcca
     2101 gccaggcgtg tgccgcggtg acgggtcggc ccaggaagat gccacgggcg atcagcgcga
     2161 ccgcggacag aagcgtcagc gaccacgcca gccggcctgc cgcctgccag tcgcgatcgt
     2221 gctcgcgcgc aagcagcagt atcagccagc agaacaggga cagcaccgcc agcgcgccga
     2281 gccagcgggc ggcaggcgaa tccacgtgga cgacgacccg caacctcgga gcaaaattga
     2341 cgtgcgattc gctcacggca acgagattac gcccgctacc tgcttgcacc agggaaatac
     2401 ttgacgatgc cctcctgcac gaccgtggcc aacaactgcc cggatcggtc gaagaaatgc
     2461 ccggtgccca gcccgcgtga atccgccgcc accggcgagg atgtggaata caacacccac
     2521 tcgtcaaagc gcaccggccg gtgaaaccac acggaatggt tagtagtgac ggcaaatatc
     2581 cggtcgtagc cccacgacag accgtgtgtg gtgatgatcg agtcgagcac cgtggtgtcc
     2641 gacgagtaca ccaacgccgc acagttcagc accgggtcgt cgggcatggc gccctcggcc
     2701 ttcatccaca cccggttgta cgtgagcttc tcccccttgt cgcgcatcac ccaggtcgga
     2761 tcgttggtgt atcgccagtc gatcggtcgt agtgcgttga cgaaatgcgg gacgacgtcc
     2821 tcgtatccac gcagaagttc gtccacgggc ggcaccgact cgggggcggg cagctcgggc
     2881 ggttcgacgc cgtgctcgat gccgcggccg ccggacaggt aggagatcat cgcggtggtc
     2941 agcagctggc cgtcctgcat cacgtcgacg cggcggttgg cgaaccggcg ttcgtcgcgc
     3001 agtcgcacga cgtggaactc gaggtccttc tcggggtcgc cgcccgcgat gaagtgggcg
     3061 gagatgctgc ttggcggcaa cttctcagac acggtacggc tgcctgcgac gaacgactgc
     3121 gcgaccatct ggccgccgaa ggtgcgcacc gggttcttac tgggatgcga cccgatgaac
     3181 gtgtcgtcgg ctacttgttt gaggtccagt accgccagca gctcctcgaa atctgccgtt
     3241 gacactcgac ctccttgatc gtcgtccgac tagacgtctt cctccccgat ccggtgcacg
     3301 tggatcagat tcgtggagcc tactgtgccg ggcggcgcgc ccgcgacgat gaccaccagc
     3361 tcgcctcgct tgtagcggcc gagctccagc aatgacttgt cgacctggcg gatcatgccg
     3421 tcggtgctcg caatatgcgg cacgatgaac gtctcggtgc cccaggtcag cgccaattgg
     3481 ctgcgcacct cgggcaggga cgtgaacgcc agcaccggca gcggcgtgtg cagccgcgcc
     3541 agcctgcgca cggtgtcacc ggactgggtg aacgcgacga gcgccttggc gtccagccgc
     3601 tcaccgatgt cgcgggcggc ataggagatg acgcctcgtt tggttcgcgg cacatgcgcg
     3661 aggggcggcg cggcgaccga attctcctcg accgcatgga tgattcgggc catcgtcttg
     3721 acggcttcga acgggaactt gcccaccgac gtctcgccgg agagcatcac cgcgtcggcg
     3781 ccgtcgagca ccgcgttcgc gacgtcggat gcctcggcgc gcgtcggccg ggaattctcg
     3841 atcatcgact ccagcatctg ggtggcgacg atgacgggtt tggcgttctc ccttgccatt
     3901 tggatggcgc gcttctgcac cagcggcact tcctcgagcg gcaactcaac gccgagatca
     3961 ccgcgggcca ccatgatcgc gtcgaacgcc agcacgatcg cctcgagatt gtcgatggcg
     4021 tccggctttt cgagcttggc gatgacgggt acccgtcgtc ccacgcggtc catgatctcg
     4081 tgcaccagct cgatgtcggc cggcgaccgg acaaacgaga gcgcgatgag gtcgacgcca
     4141 acctgcagcg cgaactccag atcctggatg tccttttccg acagcgcggg tgccgagacg
     4201 ctcatgccgg gcagcgacat acctttgttg ttgctgaccg caccgccctc ggtgaccttg
     4261 cagacgacgt cggtaccgtc gatgctctcg acgatcagac ccacgttgcc gtcgtcgacc
     4321 agcacccggt ctcctggcgt ggcgtcctcg gcgagccgct tgtaggtggt ggacacccgg
     4381 tcgtgcgtgc cttcgcaatc atcgacggtg atgcgcaccg tctcgtcgtt ggcccagaag
     4441 gtggggccgt ccttgaacct gccgagccgg atcttcggac cctgcaggtc ggcgaggatg
     4501 ccgacggcgt gcccggtcag gtcggaagcg gcgcgaaccc gcttgtaatt ggcctcgtga
     4561 tcggcgtaat cgccgtggct gaagttgagc cgtgcgacgt ccatcccggc ctcgaccagc
     4621 gccctgacgg tttcgtcggt ggaggtggcc gggcccagcg tacagacgat ttttccgcgt
     4681 ctattcacga ctgagaagca tagtcgtgaa tcggttcaga cgacggccag gggaagcgct
     4741 ccgggcttga ccggctcggg cagatccgac tcacccatca ggtaggcgtc aacggcgtat
     4801 gccgcgctgc ggccctccgc gatggcccac acgatcagcg acgcaccgcg gtgggcgtcg
     4861 ccgcagacaa acacgccggg cgcgtctgtc tgccagtccg atccgcacgg caaagcgccg
     4921 cggcggttga gtgtcaaccc gaggccgtcg agcagtggca tgtgttcgac gccgtcgaac
     4981 ccgatcgcca gcaacgccaa ttcacaggga atctcaagcg aatccccaac tggggtgatg
     5041 cgccggcggc ccccggcgtc gcgttcgacc ttcacctcgg cgatctccat ggcccgtagc
     5101 tgaccacgct cgtcgccgat gaatcgctgc acggcgacct cgtagtggcg cgcgccgccc
     5161 tcggcatgcg cgggcgacag ccgggtgcgc agcacgatcg gccacatcgg ccacggtgac
     5221 cgcgattcgt cgcggtactc cggcggctcg gggttgtaat ccagctgcgt caccgacgtc
     5281 gcgccctgcc ggtgtgcggt gccaaggcag tccgcgcccg tgtcgccacc gccgatgatc
     5341 accacgtgct tgtccttggc cgtgatcgga cttgccccgt cgccctcgca ctccttgttg
     5401 gcgggcacca ggtgctccat cgccaggtgc acacccttca gatggcggcc ttcgacctcg
     5461 ttgtcgcggg cgcgcagggc gcccactgcg agcacgacgg cgtcgtactg cttcctgagt
     5521 cgctcaacgg ggaggtcgac accgacctcg gtttcggtga cgaaacgggt tccctcggca
     5581 cgcatttggg ccagccgctg gttgagtgtg cgcttctcca gcttgtattc ggggatgccg
     5641 tagcgcatca ggccgccgat gcggtcgtcg cgctcgtaga ccgtgacgtc gtggccggct
     5701 cgcgtgagtt gttgcgcggc agccagtccc gcgggtcctg aaccgacaac ggcgaccttc
     5761 ttgccggttg agatcgccgc gggttgcggt tcgacgatgc cgtccatcca ggcctggtcg
     5821 acgatcgtct gctcgatgcg cttgatcgtg acgctgccac cggtctgctc ctcggcgatg
     5881 gacagcacgc aggcggcctc gcacggcgcc gggcacagcc ggccggtgaa ctcgggaaag
     5941 ttgttcgtgg cgtgcaggcg gtcactggcg gcatcccagc ggccacgcct gacgaggtcg
     6001 ttccactcgg ggatgaggtt gccgagcggg cagccagcgg ttcccgagtg gcagaacggg
     6061 attccgcaat ccatgcagcg gccggcctgc tgcgatacct cgccggcgcg ttggtgtgga
     6121 tcctggcgct cgtagacctc gcgccagtcg ccgacgcgct cgtcgaccgg tctcttcgcg
     6181 gcctcgacct tgggtatctt cagaaatccg gttgggtcag ccacggctgg cctccatgat
     6241 cgcggtgtcg acgtcccggc cttctcgctt ggccaccaag gtggcctcaa gtacccgctg
     6301 gtagtcgcgg ggcatgacct tggtgaattg cgcactgcgc cttggccagt cggacagcac
     6361 cgaggtggcc agagtgctgc cggtgtaggc ggcgtgcctg gcgatgacgt ctcttaacca
     6421 ggtgaggtcc tcgggctcga ggcgctgcaa ctccaccgtt tcggtgttga cccgggccgg
     6481 atcgaggccg agcacgaacg cgataccccc ggacatgccc gccgccatgt tgcggccggt
     6541 cttgccgagc accaccaccc tgccgccagt catgtattca cacgcatggt cgccgacgcc
     6601 ctcaacgacg gtcagcgcgc ccgagtttcg agcggcgaac cgctccccta cccggccgcg
     6661 caggtagacc tcgccggaag tggcgccgaa cagcagggtg ttgcccgcga tcacgttatc
     6721 ctcgggcagg aacagcacgt cgtcgggcgg ccggacgatg atgcggccac ccgaaaggcc
     6781 cttgccgaca tagtcattgg cgtcgccgat cagctccatg gtgatgccgg gcggcaggaa
     6841 cgcgccgatc gactggcccg cggagccggt gagcgtgacg tgaatggtgt cgtcgggcag
     6901 ccccgcagcg ccgtagcggc gggtgacctc ggcgcccagc agggtgccga ccgtgcggtt
     6961 cacgttgcgc acgggcagct cgagtcgcac cggatgcgca tcctcgagcg cgccctcggc
     7021 gagctggatc agcgtgcggt ccaaggcttc gtcgaggccg tggtcctggt ctctgatgcg
     7081 acgccgctgg ctcgcatctc tgggcacggt gaacaccggg gtcaggtcga ggccctgggt
     7141 cttccagtgt gcgacgccct cgacggtgtc gagcacctcg gcgtggccga cggcctcgtc
     7201 gatgctgcgg aacccgagct cggccagata atgccggacg tcttcggcga tgaaccggaa
     7261 gaagttctcg acgaactccg gcttgccgtt gaatctggcc cgcagttccg ggttttgggt
     7321 ggccaccccg acaggacagg tgtcgaggtg gcagacccgc atcatgatgc agcccgacac
     7381 cacgagcggg gcggtggcga agccgtattc ctccgcaccg agcagcgccg cgacaatcac
     7441 gtcgcgcgcg gtgcgcatgc cgccgtcgca ctgcacggtg atgcgatccc gcagcccgtt
     7501 gaggacgagt gtctgctggg tgtcggccag gccgatctcc catggcacgc cggcatgttt
     7561 gaggcttgtc agcggtgcgg cgccagtgcc gccgtcgtgg cccgagatca gcacgacgtc
     7621 ggcgtgtgcc ttggaaacgc cggccgcgac cgtgccgaca ccgacggagc tgaccagctt
     7681 cacttggatg cgggcctcgg agttggcgtt cttcaggtcg tggatcagct gggcgaggtc
     7741 ctcgatcgaa tagatgtcgt ggtgcggtgg cgggctgata agaccgacgc cgggggtcga
     7801 gtggcgggtc ttggcgatgt tcggatacac cttgaagccc ggaagctggc ctccttcacc
     7861 gggtttggcg ccctgcgcca tcttgatctg gatgtcggtg gcgttgacca g
//
LOCUS       DASRJA010000727        24791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3385724,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000727 DASRJA010000000
VERSION     DASRJA010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 24791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24791
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3385724"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFC01_32840"
     CDS             complement(<1..703)
                     /locus_tag="VFC01_32840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN84443.1"
                     /translation="MRVLLTGAAGFIGTRVAVALTAAGHEVVAVDGMLKSAHGPNAQP
                     PPDVQQVDVRDAAALAPLLNGVDVVCHQAAVVGAGVDTADAPSYCSHNDFATAVLLAE
                     MFAAGCQRLVLASSMVVYGQGRYECAEHGLVDPVSRTRADLDAGVFEHRCPLCGQPVR
                     WRLVGEDAPLRPRSLYAASKTAQEHYALAWAESTGGSVVALRYHNVYGPGMPRDTPYS
                     GVAAIFRSELEAGDVP"
     gene            complement(700..1476)
                     /locus_tag="VFC01_32845"
     CDS             complement(700..1476)
                     /locus_tag="VFC01_32845"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HZN84444.1"
                     /translation="MLGVIGGSGFYTFFGSDARTINLDTPYGEPSAPITVGTVGEHEV
                     AFLPRHGVKHEYSPHAVPYRANMWALRALGVRRIFGPCAVGSLTPELGPGAMVVPDQL
                     VDRTSGRDDTYFDSGGIHVGFADPYCPTLRAAAAGLPGVVDGGTMVVVQGPRFSTRAE
                     SRWFASQGFTLVNMTGYPEAVLARELEMCYAAIALVTDLDAGIDAGTAVRAVDVFAEF
                     ERNLVPFKKLVHEAIDQVTSERTCTHCLPHDGVELPFDLP"
     gene            1510..2379
                     /locus_tag="VFC01_32850"
     CDS             1510..2379
                     /locus_tag="VFC01_32850"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HZN84445.1"
                     /translation="MATFAQWVEGARPRTLPNAIAPVIAGTGGAAWLHAACWWKALLA
                     LVVAVAMIIGVNYANDYSDGIRGTDDVRAGPLRLVGSKLAAPRAVLTAAVVSLGIAAV
                     AGVVLAWLSAPWLIAVGALCIAGAWLYTGGSKPYGYLGLGEVAVFVFFGLVAVLGTQY
                     TQALRVDWVGLALAVAMGSLSSAVLVANNLRDIPTDKESGKITLAVRLGDARTRLLYQ
                     ALLVVAFALTLVLMLATPWCAVGLVALPLAVRAAGPVRKGLGGKELIPVLRDTGLTML
                     VWAVAVALALAFG"
     gene            complement(2540..2884)
                     /locus_tag="VFC01_32855"
     CDS             complement(2540..2884)
                     /locus_tag="VFC01_32855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN84446.1"
                     /translation="MTTAKALYQRWIDELWTGKSVAAELVSDDFVAHWPDREVRGPGE
                     LQEIVDETQKMFTDLMFVIEVEPFVERDMVAARWIGTGATKEGPARFTGNDILRFTDD
                     RFVEYWTGTSTA"
     gene            complement(2972..3475)
                     /locus_tag="VFC01_32860"
     CDS             complement(2972..3475)
                     /locus_tag="VFC01_32860"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HZN84447.1"
                     /translation="MTVGVGLHSGTPFALSIGTVLMAVIFAGGHRIGAHFNPAITLAA
                     AIWGRIPFGEAGAYWLAQLAAGLCAVITTRLVVGSGQSEAVMEMMLSGRALFAALGAE
                     LLFVFSCIESPRAAPNTLWHFAIGVAVVAGTVDFAALFGSVSMVNQVIAGAFTGIAFL
                     TFGSAAR"
     gene            complement(3835..4071)
                     /locus_tag="VFC01_32865"
     CDS             complement(3835..4071)
                     /locus_tag="VFC01_32865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84448.1"
                     /translation="MKLTYLTALFGAAAAAVAIFAAPVAAADTGQTCSGNGSGTICQS
                     PGNAQINDAPPPVNFYPYGGEAFLLGGGGFRVGR"
     gene            complement(4270..5469)
                     /locus_tag="VFC01_32870"
     CDS             complement(4270..5469)
                     /locus_tag="VFC01_32870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN84449.1"
                     /translation="MTTTPRHSLPQRLREGRVGQAPFGPAGATSTDRQLHNRFRGAAL
                     FVAALAVAVVSAGMGGVAALNVYPHQAVRSTMTTAAASQPAGAIEQIAAKVLPSVVQL
                     QSDLGDHTELGSGIILTPDGLIMTNAHVVSAAQNADPAGPGGARTLVSLADGRAVPFA
                     VVAADPTSDIAVVRAEATSGLTPITFGSSADLRVGEQVAAVGSPLGLDGTGTSGIISA
                     LNRPASTSTSDSASQGAVIDAIQTDAPINPGNSGGALVNANGQLIGMNSANASFGGAS
                     GESGSIGLGFAIPVDEAKRIADELIATGTACHGSLGAQVTDVTNAYGARIVGVTSGGP
                     AAAAGLAAGAVITKVDEQVINNTDALVAGVQSKAPGTTIALNYLDPSGSPRSVQVLLG
                     TDQGQQS"
     gene            complement(5707..6069)
                     /locus_tag="VFC01_32875"
     CDS             complement(5707..6069)
                     /locus_tag="VFC01_32875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003938125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN84450.1"
                     /translation="MSESNHYHRVLEDLNPQSRVLRQMIPEVYRGFAEMNTAALASGA
                     LDRKIKELIGLAIGVVHRCDGCIASHAKGAARAGASEQEAAEAIGVSILMQGGPATVY
                     GARAYEAFCEFAAATEKV"
     gene            complement(6167..6403)
                     /locus_tag="VFC01_32880"
     CDS             complement(6167..6403)
                     /locus_tag="VFC01_32880"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN84451.1"
                     /translation="MTNLPLNGIKVIDSTGVQAGPACTQMLAWFGADVLKVERTTGGD
                     VTRHQLRDIPDVDALYFTMLNSNKAVVGDQYQNR"
     gene            complement(6437..8155)
                     /gene="oxc"
                     /locus_tag="VFC01_32885"
     CDS             complement(6437..8155)
                     /gene="oxc"
                     /locus_tag="VFC01_32885"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HZN84452.1"
                     /translation="MSTEITATADTAAELTDGYHLVVDALTLNGVRTVYGVVGIPITD
                     VARVAQASGIRYVGFRHESDAGHAAAAAGFLTKKPGICLTVSAPGFLNGLVALANATT
                     NCFPMVQISGSSERHIVDLKRGDYEELDQLAAAQPFAKAAYRVNRVEDIGRGVARAIR
                     TAVSGRPGGVYLDIPAAVLGEVIDAAAAAASLRRVVDAAPRQLPAPEAVDRAIGLLAN
                     AQRPLVVLGKGAAYSQADKEIRAFVEATGVPFLPMSMAKGLLPDDHPQTAAKAKSLAL
                     RRADVVMLVGARMNWLLGHGEAPQWNPDTQFIQVDIEPAELDSNQPIAAPLVGDIGSV
                     MQALIERTQPDEVRAPAAWRDELAERSGQNVAKMSQRLTADPHPMQFYSALRAIRDVL
                     HHNPHVYVVNEGANALDFTRNVVDMSVPRHRLDSGTWGVMGIGMGYAIAAAVESGGPV
                     VAIEGDSAFGFSGMELETICRYELPIVVIVLNNGGVYRGDGINTVSTDPSPTTLMLSA
                     RHDRLIEAFGGKGYQVTTPAQLTIALTGALMSGRPTLIDCVIDPTDGTESGHLTRLNP
                     TGVGKK"
     gene            complement(8145..8999)
                     /locus_tag="VFC01_32890"
     CDS             complement(8145..8999)
                     /locus_tag="VFC01_32890"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HZN84453.1"
                     /translation="MARKSHGGDMSPEAPIRLDILSASTTAGSQDRQSVRDLLLVALT
                     VASGAVDAISYFGLGKIFSAFMTGNLVFLGFGIAEIEGPDVVPVIVALSMFTVGAYFG
                     LRFTTLRSNESGLWSPAVTVLLVLVAVAEASFLVAWLSTAEHPSTGIVDVLIALFSLA
                     MGIQTAAVRSLGVQGIFTTAGTFTLVAFAGTFAGSRSRSEMPRLVGVLLGLVAGAVAG
                     GLLFLHARSYAPALPLVITVLVILAGRSMQRSRAALGETHGGANQATPEVKAHSASRT
                     RRASAHEH"
     gene            9436..10209
                     /locus_tag="VFC01_32895"
     CDS             9436..10209
                     /locus_tag="VFC01_32895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84454.1"
                     /translation="MTVDNHSRARLEPVAVKVPEIAASFWVLKLLTTAMGEAASDYLL
                     TTMNLIGLGVGLAGFALALWVQLRTRRYNAFAYWAAVMMIAVFGTMAADTLHHQLGVS
                     FGLSTLVCALAVAATFWAWYRVEHTLSIHSITTRRREVFYWLTVSFTFALGTAVGDLT
                     ASSLHFGFVGSIVLFAVVMAVPALGYWRFRLNSVIAFWWAYIVTRPLGASVADWLSKP
                     AKSGGLAYGDGPVAAMLLAFALMLVTVAAVSRRAPTFEA"
     gene            10206..11792
                     /locus_tag="VFC01_32900"
     CDS             10206..11792
                     /locus_tag="VFC01_32900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HZN84455.1"
                     /translation="MTVPFSWQLPYAWPRTPVLARNVVCTSQPLAAQAGLAMLAEGGS
                     AIDAALAAAVTLTLVEPVSNGIGSDAFAIVWDGRQLHGLNASGRSPAAWTQEYFQGRP
                     VPARGWNSVTVPGAVSAWVELHAKFGKRPFARIFEPAIGYGRDGFLVSPTIASQWAAQ
                     VPDLRSQPGFGEAFMPGSRAPSPGELFRLPEHAATLEKIAATNGEAFYRGELAETMAA
                     HSAANGGAMIAADLAAHRADWVGTITTDYRGYTIHELRPNGQGIVALVALGILEHFDM
                     ASLPVDSADSVHLQIEALKLAFADALAYVADIDHMQVRPGQLLHKEYLKQRSTLIDRT
                     QAQDVTAGAPPKGGTVYLTAADASGMMVSMIQSNYMGFGSGVVVPGTGISLQNRGTGF
                     VTTPGHPNLVGPNKRPYHTIIPGFMTKDGAPVMSFGVMGGTMQPQGHLQVVVRIADYG
                     QNPQAACDGPRFRWVQGMQVSVEGGFPPATLEGLRRRGHDIVTVDDYNQFGSCQAIWR
                     LDDGYLAASDPRRDGQAAGL"
     gene            12107..14497
                     /locus_tag="VFC01_32905"
     CDS             12107..14497
                     /locus_tag="VFC01_32905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HZN84456.1"
                     /translation="MTNGKTHISGGLLGQGEAGRPAAGRGPNAGGQFSQEDYHHPAAG
                     WGAARSVAKVLAHSRELVAGTRAIQLMNHENGGFDCPGCAWPDDTKGLHLDICENGIK
                     HVTWEMTRKRVDREFFASHTVSDLSGWTDFALEDQGRLTEPMVYDAESDRYVPISWPD
                     AFGLVGSTLRRLDSPHQASFYTSGRLSNEATFLYQLWVREFGTNNLPDCSNMCHEASG
                     RAMTASLGTGKGTCDLDDWENADLLLVMGVNAASNAPRMLTSLAEAHRRGAQIVHVNP
                     LIEAASRRTIVPHEIVAMAAFHATKIGTMNVQPRVGGDLALLRGVAKVVLERAQSDPK
                     AIDREFVARYTTGFEAYRSLVESTGWSEIVQQSGVEEATIRRLAEVYLGADRTVVAWC
                     LGLTQQEHGVDTVREIVNLLLLRGNIGREGAGPSPVRGHSNVQGNRTCGIDHRPPKQL
                     LDRLEEVCAITPPREHGLDTVGTIKAMLAGDVKVFVSMGGNFSLATPDTAATFAALRN
                     CELTVQVSTKLNRSHLVHGRKALILPCLGRTEKDVQRTGEQGVTVEDSMSMVHLSFGM
                     KSPASPRLKSECGILAGMARATLPASATPWEYYVEDYDRIRDTMAKVFDGFEDFNRRV
                     RLPLGFRIRQPARERVFLTASGSAEFSAAPMPDVVPPPGRLTLSTVRSHDQWNTTIYS
                     DDDRYRGLKNLRTALFMNADDMREHGLDEFDLVDITSFARDGSTRSVYGYRAVAYDTP
                     RGSVFGYMPELNLLCALADHSEQSGQPLTKQLIVEVSPATAGPATTPTGAPAAGRS"
     gene            complement(14616..14831)
                     /locus_tag="VFC01_32910"
     CDS             complement(14616..14831)
                     /locus_tag="VFC01_32910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84457.1"
                     /translation="MRIRLTYITPLFGTAAAAVAILAAPIAAADTGQSCSADGSGTIC
                     QSPGNAQINDAPPPVNYYPYGGEAFLL"
     gene            complement(15003..16280)
                     /locus_tag="VFC01_32915"
     CDS             complement(15003..16280)
                     /locus_tag="VFC01_32915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016887629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HZN84458.1"
                     /translation="MSVTTPIRTDRSTAPHPRAALIRSALITTVFIMPALVLRVVGLH
                     PNPVLALCVYGVAVVSASFLLAWAAEAAQIDVSGGLAIAVLALIAVLPEYAVDLYYAY
                     TAGHAPDYVQYAAANMTGSNRLLMGLGWPVVVLVALAVARKLNNGKTSALQLDPGNRV
                     ELGFLLIAGVVAFGIPVSGHIDITIGVALLGWFGFYLYRLTRGDVEEPELIGTAAAIG
                     GLPDRVRRTTVVALFAVAAAVILLSAEPFAESLIGAGTELGIDRFLLVQWLAPLASEA
                     PEFVIAILFAARGKGTAAIAMLISSKVNQWTLLVGSLPVAYMIGGGGTVLQLDGRQIE
                     EVLLTATQTMMGVALILALRFHRYTAWALLALFAVQFPITSTAGRLILCGVYAALAIA
                     GFIVNRRHLTATLKSLFDDAARHDRSADQGQSS"
     gene            complement(17032..17985)
                     /locus_tag="VFC01_32920"
     CDS             complement(17032..17985)
                     /locus_tag="VFC01_32920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HZN84459.1"
                     /translation="MTNVAEYGFEGRFEDWAEDARYFEYSKAANPTGSGHTSKIPIRA
                     FSRELYSDGPTQIIPLDLSHELGIAHGPATSPALVANFVRIRAGEHIATDPNATSQLY
                     YVISGRGFSAVNGRLVQWEKGDFLTLPARSQATFYAHSDAALYWVHDEPLLRHLGAEA
                     AEPRFNATKFRREDAVGRLNEIASRPGANDKSRVSVLLANAEQEQTLTITHVLWAMFG
                     LLPPDQVQRPHRHQSVALDLILDAEPGCYTLLGSRLDERGDIVNPTRVDWEAGGAFVT
                     PPGLWHAHFNESGAPAHLIPVQDAGLQTYLRSLDIRFSDRR"
     gene            18301..19290
                     /locus_tag="VFC01_32925"
     CDS             18301..19290
                     /locus_tag="VFC01_32925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012835859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HZN84460.1"
                     /translation="MNVLLVGTIEPSLAKAVGAKYKALQLPNAATRRRFLAKHGGSIT
                     AIVDGGPPGVDANLMEALPNLGAIVHQGAGYDTIDIDAARRLGIGVSNTPDVLNDTVA
                     DTAIGLMLATMRGLCTADNFVRSGRWPLDGNYPLGRDLSGSRVGILGLGRIGSAIAQR
                     LVGFDCSIAYHSRRQVPGTPFRYVTSPVELAESVDVLVVATVGGPGTKHLVNRTVLEA
                     LGPYGYVINIARGSVVDQDALVELLVARRLAGAGLDVFADEPYVPTELCGLDNAVLLP
                     HIGGATVRSVSMMRELVLRNLDQYLNNGTLTTPVVPSNRRARNGIHKCSGFRP"
     gene            19325..20965
                     /locus_tag="VFC01_32930"
     CDS             19325..20965
                     /locus_tag="VFC01_32930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD7 family fatty acid--CoA ligase"
                     /protein_id="HZN84461.1"
                     /translation="MAAPTETPIHAANQYRPPAVSGLFDLLDQHAHDRPDARALVAGS
                     DRTPISYGELAALVKDVANRLANSGLRRGDAVGVVGANNPDFVVALLGATRAGLVVAP
                     LDPALPRSEMTTRLERLGAQALLVGRPALDAEPVAPLGIPTWNLEVHIAAHRTPAVTL
                     DAGALRRSEPKSGLSERDALVLFTSGTTAQAKMVPLTHENVAASVRAICVSYELGPDD
                     ATVAVMPLFHGHGLIAALLASLASGGCVLLPERGRFSARSFWGDMRAASATWFTAVPT
                     IHQILLQRSASDYPGANAVPLRFVRSCSAPLNTAIARAMERTFGAPVLSAYGMTETTH
                     QAASQTVRGNVKEGSVGSATGVSLRVVGPDGRRCPAGTEGEVWVQGPTVARGYLRDPA
                     ETAHNFVDMWFRTGDLGAIDEEGYLFVTGRIKNIINRGGEKISPEHVEAVLARCRGVT
                     EAAVFAIPDATYGERVGAAVVVPQDESFSSDQILQECRGTLAAFEMPERLEVVAALPH
                     TAKGALDRRAVAEQYVRCARSDDDPLCRRRPIEELVEG"
     gene            complement(20909..21841)
                     /locus_tag="VFC01_32935"
     CDS             complement(20909..21841)
                     /locus_tag="VFC01_32935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZN84462.1"
                     /translation="MLFRQLEYFVAVARERHFARAAEVCYVSQPALSASIAKLERELN
                     VTLINRGHNFEGLTMEGERLVVWAKRILAEQEAFKAEVAAVQSGITGTLRLGTEPTAS
                     TTQTLPVAAFCAAHPLAKVQVESRLSTTELCRQLLDFELDAAIAHFDSEEQSCLRAVP
                     LYEERYILLAPDDLPVPPDPTMTWADAAQLPLVLLTPDMRIRRVIDCAFAQSGVTVTP
                     QVETDSVASLYAQVGVGAWASIVPHTWLRAMPVVGRARAVRLVEPDVSAQISVATHAA
                     TPGSVVARAFVSAAEGLALDEFFDRTAPTERIVI"
     gene            22049..22702
                     /locus_tag="VFC01_32940"
     CDS             22049..22702
                     /locus_tag="VFC01_32940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN84463.1"
                     /translation="MSRVRVVVADDDLLLREGLASLLDRSGFEVVGQAGDGRELLAMV
                     RETTPDLVVADIRMPPTHTTEGLDAARVIREEFPDIAIMVLSAHVDVEHAMELLASGR
                     SIGYLLKNRVTDVTDFLDTLERIANGASVVDPALVSELVSARRRDDPLAALSSREREV
                     LALMAEGRSNAGIAHRIWVTEGTVEKHVRSILTKLDLAETGDDHRRVLAVITFLETR"
     gene            complement(22670..23056)
                     /locus_tag="VFC01_32945"
     CDS             complement(22670..23056)
                     /locus_tag="VFC01_32945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZN84464.1"
                     /translation="MRCLIVDDSADFVEAACGLLECEGITVVGVASTSADALRLFAES
                     RPDVTLVDINLGGESGFELAEQLHRTGGPSPSPVILISTHAAQDYTDMIETSPAVGFL
                     SKSALTCGAIHDLVGESARLEEGDHR"
     gene            23295..24272
                     /locus_tag="VFC01_32950"
     CDS             23295..24272
                     /locus_tag="VFC01_32950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN84465.1"
                     /translation="MKTTTLGRSGLKVSRIAFGTWQLGGDWGEFDENAAVAAIRRARE
                     LGVNFFDTAQAYGFGASEAILGKALRDEFSRDRDELVIATKGGLRQTDSGLVRDASPE
                     WLCRGVDASLTALGTDHIDLYQVHWPDPTVPAAETAGALSDMITEGKIGHVGVSNYNS
                     AQMAEFSATLPVETLQPPYHLFRREIEDEVLPYCRDNNVGVLVYGPLAHGLLTGTLSV
                     HTAFEDGDWRGTSKVFRGGTYRRNLGAVRELEKFAANRGITVSQLAIAWTLANPAVHV
                     AIVGARQVGHIEDSLRAVDISLTDSDLDEIELIMEEATPVAGPSPEGMP"
     gene            24269..>24791
                     /locus_tag="VFC01_32955"
     CDS             24269..>24791
                     /locus_tag="VFC01_32955"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN84466.1"
                     /translation="MTGPAVTVAVLGTGTMGAPIARNLLHAGFDVRVWNRTVAKAAMV
                     GVDAHRTSTPGEAAAGADVLITMLADGAAVEQVMTGPTGALSKLGAGAIWIQMSTVGA
                     EWTDRLAGLVARHDVAFVDAPVSGSSAAADKGELVILASGAITIRSRVEPIFDVLGHQ
                     TLWLQSTGDGSRLK"
BASE COUNT         4424 a         8004 c         8034 g         4329 t
ORIGIN      
        1 ttggtacgtc gcctgcttcg agttcagagc ggaaaatcgc cgccaccccg gaatagggtg
       61 tgtcgcgtgg catgccgggg ccgtagacat tgtgataccg cagcgcgacc accgatccgc
      121 ccgtcgactc ggcccaggcc agcgcgtagt gctcttgcgc ggtcttgctc gccgcgtaca
      181 gactgcgcgg ccgaaggggc gcgtcctcgc cgaccagccg ccaccgcacc ggctgtccgc
      241 agagcgggca gcgatgctcg aacaccccag cgtccagatc ggcacgggtg cgcgaaaccg
      301 gatcgacgag cccgtgttcg gcacattcgt accggccctg cccgtagacc accatcgacg
      361 aggccaacac caaccgctgg cagcccgccg cgaacatctc cgcgagcagc accgccgtgg
      421 cgaagtcgtt gtgactgcag taggacggcg cgtcggcggt atccaccccg gcgccgacga
      481 ccgcggcctg atgacagacc acgtcgacgc cgttgagcag tggcgctaac gcggcggcgt
      541 cacgaacgtc gacctgctga acatccggcg gcggttgcgc attggggccg tgcgcgctct
      601 tgagcatccc gtcgacggcc accacctcgt gaccggcggc ggtcagcgca acggccaccc
      661 gcgtcccgat gaatccggcg gcgccggtca gcagcaccct cacggcagat cgaaaggcag
      721 ctcgacgccg tcatgcggca ggcaatgtgt gcaggtgcgc tcgctggtca cttgatcgat
      781 ggcctcgtgc accagcttct tgaacggcac caggttgcgc tcgaactcgg cgaatacatc
      841 gacagccctg acggccgtcc ctgcgtcaat gcccgcatcc aggtcggtca ccaaagcgat
      901 agcggcgtaa cacatttcga gttcgcgggc cagcaccgcc tcgggatagc cggtcatgtt
      961 gaccagggtg aagccctggc ttgcgaacca gcggctctcg gcgcgcgtgg aaaaccgcgg
     1021 tccctggacc accaccatcg tgccgccgtc gaccacaccc ggcaggcccg cggccgccgc
     1081 ccgcagcgtc gggcagtacg gatcggcgaa cccgacgtgg attccacccg agtcgaagta
     1141 ggtgtcgtcg cggccgctgg tgcgatccac caattggtcg ggcaccacca tggcgccggg
     1201 cccgagctcg ggggtgaggc tgccgaccgc gcatggcccg aagatccggc gcacacccag
     1261 cgcccgcaac gcccacatgt tcgcgcggta gggcacggca tgcggggagt actcgtgctt
     1321 gacgccatgc cggggcagga acgcgacctc gtgctcgcct acggtgccga cagtgatcgg
     1381 ggcgctcggc tcgccgtacg gggtgtcgag gttgatggtg cgggcgtcgg aaccaaagaa
     1441 cgtgtaaaaa ccgctgccgc cgatgactcc caacactccc ccattttgcg ccgtgcgacg
     1501 atgacgctcg tggccacttt cgcgcaatgg gtcgagggcg cccgcccgcg aacgttgccc
     1561 aacgcgatcg ccccggtgat cgcaggcact ggcggcgcgg cctggctgca cgccgcgtgc
     1621 tggtggaaag cgctgctggc gctggtcgtc gcggtcgcga tgatcatcgg cgtcaactac
     1681 gccaacgact actccgacgg cattcggggc accgacgacg tccgggccgg cccgttgcga
     1741 ctggtgggct cgaaactggc cgcgccgcgt gcggtgttga cggccgccgt ggtgagcctc
     1801 ggtatcgcgg cggtggccgg ggtggtgctg gcgtggctca gcgcgccgtg gctgatcgcg
     1861 gtcggcgcat tgtgtatcgc cggggcatgg ctctacaccg gtggctcaaa gccgtatggc
     1921 tatctggggc tcggcgaagt cgcggtgttc gtgttcttcg gcctggtcgc cgtgttgggc
     1981 actcagtaca cccaggcgtt gcgggtggat tgggtggggc tggcgctcgc ggtggcgatg
     2041 ggctcgctgt cgtcggcggt gttggtggcg aacaatctgc gcgacatccc gaccgacaag
     2101 gagtccggca agatcacctt ggccgtgcgg ctgggcgacg cccgtacgcg gctgctgtat
     2161 caggcgctgt tagtggtcgc gttcgcgctg acgttggtgc tgatgctcgc gacgccgtgg
     2221 tgtgcggtcg gattggtggc cttgccgctg gcggtgcggg cggccggccc ggtgcgaaag
     2281 ggactcggcg gcaaggaact tatcccagtg ctgcgcgaca ccgggctgac gatgctggtg
     2341 tgggccgtcg cggtggcact ggctctggcg ttcggctaag cgatatcggc actcgcgacc
     2401 cgacgagtcc gccgcgtggc cagttattgc acgccttttc gcgatatgcg tgacataact
     2461 ggccactcac ggtgcaggtc gtggcccgcc gcgtctgctg gcgccgttgt ttttgccgta
     2521 tgggatcgcg ctgcggacgc taggcggtgg aggtgcccgt ccagtactcg acgaatcggt
     2581 cgtcggtgaa ccgcaggatg tcgttgccgg tgaaccgcgc cggaccctcc ttcgtcgccc
     2641 ccgtgccgat ccagcgggcg gccaccatgt cccgctcgac gaatggctcg acctcgatga
     2701 cgaacatcaa gtcggtgaac atcttttgcg tctcgtcgac gatctcctgc aactcgccgg
     2761 gaccgcgaac ctcacgatcc ggccagtgcg caacgaaatc gtcggagacc aactcggctg
     2821 cgacggactt gcccgtccac aactcgtcga tccatcgctg gtacagcgcc tttgcggtag
     2881 tcatacgcga cgtctacccc gccacgacgc aatctatgcg atcgcaacga gatgacgaac
     2941 gtcgcaccca agatgtgcag ctcgacacag atcatcgggc cgcggaaccg aaggtcagaa
     3001 aggcgattcc ggtgaaggcg cccgcgatca cctgattcac catggacacg ctgccgaaca
     3061 acgcggcaaa gtcgaccgtg cccgccacca ccgcgacgcc gatcgcgaag tgccacagag
     3121 tattcggcgc cgcgcgcggc gactcgatgc agctgaacac gaacagcaac tcggctccca
     3181 acgccgcgaa cagagcgcgc ccgctcaaca tcatttccat cacggcttcg gattggccgg
     3241 aaccgacgac gagtcgggtg gtgatcaccg cgcataaccc ggcggcaagc tgtgccaacc
     3301 aataggcacc cgcctcaccg aacggaattc ggccccatat cgccgcggcc aacgtgatcg
     3361 cgggattgaa atgcgcaccg atccggtggc cgccggcgaa gatcacggcc atcaggaccg
     3421 taccgatgct cagagcaaac ggtgtgccgc tgtgaagacc cacaccgacg gtcatcaccg
     3481 ggccaaacgt tcccagtgct tcgacgacgt acttgttcag cgtcctagac ttttcgccga
     3541 cgcttggcgt gtaggctcat ccagccagtg cggggcggcg gtcatggagt acaaccctgc
     3601 ggctttacgc cccaatggcg tcaccgctga ccgccatata ccatggctga ccgaccacgc
     3661 cctgggagta ttacgtcgag gactacgacc gaaatccgcg ctaccggtcg gtgagctacg
     3721 actccagccg cggcagcctg atcggttaca tgcccgagct gatcaaagcc agcagagcaa
     3781 tcagccgttt actaaacacc tcgtcgtcga tgtcaccctg ccagcggctg acccctaccg
     3841 tccgacgcga aagccgccgc ctccgagcag gaatgcttct ccgccatagg gatagaagtt
     3901 gacgggcggg ggggcgtcgt tgatctgcgc gttgccgggc gactggcata tggttcccga
     3961 tccattcccg gagcaggtct gcccggtatc ggccgcggca actggtgcgg cgaaaatcgc
     4021 cactgcggcc gctgcggctc cgaacaatgc cgtcaagtag gtcagtttca tggtgtactc
     4081 ccgtcattgg gtagcccgaa tctggactaa ttccaggatg gccctcggcc tacctcacgt
     4141 cgtccctgct ggcagccatc cacactccct gctgactgga atctttcgta ccgacagccg
     4201 tcagtgccga atatcgattc ggattctggg acaagtggat tcagcgtcgg catcagtcga
     4261 cagcagactt catgactgct gcccctgatc cgtgccgagc aagacttgca cactacgggg
     4321 gctaccggac gggtccagat aattcagtgc gatagtcgtg ccgggcgcct tcgactgcac
     4381 tccggcgaca agtgcgtcag tgttgttgat cacttgctcg tctacctttg tgatcaccgc
     4441 accggcggcc aggcctgccg ccgcagcggg acccccactt gtcactccga caatccgggc
     4501 accgtaggca ttcgtgacat cggtcacctg tgcacccagg gacccgtggc aggcggtgcc
     4561 ggtggcgatg agttcgtcgg cgatgcgttt ggcctcgtcg acgggaatcg cgaaaccaag
     4621 gccgatcgaa ccactttcac cggaagcacc gccgaacgac gcgttcgccg agttcatccc
     4681 gatcagctgg ccgttcgcat tgaccaacgc cccgccggag ttgccgggat ttatcggagc
     4741 gtcggtttgg atggcatcga tcaccgctcc ctggctcgcg gagtcagacg tgctagtcga
     4801 cgccggccgg ttcaacgcgc tgatgatgcc cgaagtgccg gtgccatcca ggccgagcgg
     4861 cgaacccacc gcggcgacct gctcgccgac gcgaaggtcg gcggacgagc cgaacgtgat
     4921 cggagtgagt ccagacgttg cctcagccct cacgacggcg atatcactcg ttggatcggc
     4981 ggcgacaacg gcgaagggca ccgcgcggcc gtcggcaagg ctcaccaggg tgcgtgcacc
     5041 acccgggccg gccgggtctg cattctgtgc cgccgacacg acatgggcat tggtcatgat
     5101 cagcccgtcg ggcgtcaaga tgatgcccga acccagttca gtgtgatcgc ccagatcgct
     5161 ctgcagttgc acaacgctgg gtagcacctt tgcggcaatc tgttcgatcg cgccggccgg
     5221 ctgtgacgct gccgcggtcg tcatggtgct ccggacggcc tggtgcggat acacgtttaa
     5281 tgcggcgact ccgcccatac ccgcggacac gaccgcaacc gccaacgctg ccacgaagag
     5341 tgccgcgcct cgaaaacggt tgtgcagctg tcgatctgtc gaggtcgccc cggcgggccc
     5401 aaatggggcc tggccgactc ggccttctcg aagtcgttgg ggaagcgagt gcctcggcgt
     5461 ggtcgtcacg tctccgcggc cggagggtag cgtgaacacg gtttgtagaa tcgcggtcgt
     5521 cggtgcctgt tgtcgctaac gctggcgccc attgccgctt gctgccagcg tcaaatcagt
     5581 catacccgcc agctgcaaat aagtgactcc tcggctcaac cgaaggagca cctgcagccc
     5641 gaatgggggg tcgagcgccc gcatctgtca gaactgcaag tcacgacgat gccacaaggt
     5701 ggacccctag accttctcgg tagcggcggc gaactcgcag aatgcttcgt aggcgcgggc
     5761 accgtaaacg gtcgcgggcc caccctgcat gaggatgctt acaccgatcg cctcggcggc
     5821 ctcctgttcc gatgcccctg cacgagcggc ccctttcgcg tgcgaggcga tacaaccatc
     5881 gcaccgatgc acgactccga tggccaggcc gatgagctct ttgatcttgc ggtccaaggc
     5941 gccggatgcc agtgctgcgg tgttcatctc ggcgaaaccg cgatatacct ccgggatcat
     6001 ctgccgcaac acacggcttt gcgggtttag gtcttcgagc acgcggtgat agtggtttga
     6061 ctcgctcatg gacgtttcct tatctgtgtt gttcaggcgt tgtgtttaga aagccgcggc
     6121 aagatccgag cgggctgatc caagcttctc cgtgacttct ttaccctcag cggttttggt
     6181 attgatcgcc aacgaccgcc ttgttgctgt tcagcatcgt gaagtacaaa gcgtcgacgt
     6241 cgggtatgtc gcgcagctga tggcgggtca cgtcaccgcc cgtcgtccgc tcgaccttga
     6301 ggacgtcggc gccgaaccac gcgagcatct gagtgcacgc cgggccggct tgaacgccag
     6361 tggaatcgat gaccttgatt ccgttcagtg gcaggttagt catgatgggt tcccttctag
     6421 agggggatct ttggtgtcac ttcttcccga caccggtcgg gttgagacgg gtgaggtgac
     6481 cgctttcggt gccgtcggtg ggatcgatca cgcaatcgat gagagtgggg cgtcccgaca
     6541 tcagcgcacc ggtgagggcg atggtgagtt gcgcgggcgt ggtcacctgg tagcccttgc
     6601 caccgaacgc ctcgatcagg cggtcgtgtc gggcactcaa catcagggtt gtcggggacg
     6661 gatccgttga tacggtattg atcccgtcgc cgcggtagac cccgccgttg ttgaggacga
     6721 tgacgacgat gggcagctcg tagcgacaga tcgtctccag ctccatgccg ctgaatccga
     6781 acgcgctgtc accctcgatc gccacgacgg gaccgccgct ttccacggct gcggcgatcg
     6841 catagcccat gccgatgccc atcactcccc acgtaccgct gtcgagacga tgtcggggca
     6901 ctgacatgtc gaccacgttg cgcgtgaagt cgagcgcgtt tgcgccttcg ttgacgacat
     6961 aaacgtgagg attgtgatgc agcacatcgc ggatggcccg cagcgcgctg tagaactgca
     7021 tcgggtgggg atcagcggtc aggcgctgcg acatcttggc gacgttctgc cccgagcgtt
     7081 cggcgagctc gtcccgccat gctgccggag ctctgacttc gtcgggctgg gttcgctcga
     7141 tcagcgcctg catgaccgag ccgatgtccc caaccagcgg tgcggcaatc ggttggttgc
     7201 tgtcgagctc ggccggttcg atgtcgacct ggatgaactg cgtgtcggga ttccactgcg
     7261 gcgcttcgcc gtggcccagc agccagttca tcctggcgcc gaccagcatc accacatccg
     7321 ctcgccgcag ggcgagcgac ttggctttgg ccgccgtctg cggatggtcg tcaggcagca
     7381 ggcctttcgc catcgacatc ggcaggaacg gcacgccggt cgcctcgacg aatgcccgga
     7441 tctccttgtc cgcctgcgag tatgcggcac ccttgcccaa taccaccagc ggccgctgcg
     7501 cgttggccag cagaccgatc gcgcggtcca cggcttcagg ggcgggcagc tgtcgggggg
     7561 ccgcgtcgac gacccggcgt agtgacgcgg cggccgcagc ggcatcgatg acctctccga
     7621 gcaccgcggc tgggatatcc agatagaccc cgccggggcg accggacacg gccgtccgaa
     7681 tcgctcgggc gacgccgcgg ccgatgtctt cgacccggtt cacccgatag gcggccttgg
     7741 cgaacggctg cgccgccgcc agctgatcga gctcctcgta gtcgccccgc ttgaggtcga
     7801 cgatatgtcg ctcgctcgac cccgaaatct ggaccatcgg aaagcagttc gtggtcgcat
     7861 tggcaagcgc gacgaggccg ttgagaaagc ccggagcgga taccgtcagg cagatacctg
     7921 gcttcttggt caagaaccct gctgccgccg ccgcgtggcc cgcatcgctc tcatgccgaa
     7981 agccgacgta gcggatgccc gacgcctgcg cgacgcgcgc aacgtcggtg atcgggatgc
     8041 cgaccacgcc gtagacggtc cggacgccgt tgagtgtgag ggcatcgaca accaggtggt
     8101 atccgtcggt gagctcggca gccgtgtcgg ctgtcgctgt aatttcagtg ctcatgagcg
     8161 gaggccctcc tggtgcgact ggcgctgtgc gcctttactt ctggcgttgc ctgattcgca
     8221 ccgccgtgtg tttcgccgag agctgcccgc gacctttgca tcgaccgccc cgccaggatc
     8281 accaggacag tgattaccag cggtagagcc ggcgcgtagc tgcgcgcatg caggaacagc
     8341 agacctcccg ccaccgcgcc cgcgaccaac cccaagagca cgccgacgag gcggggcatc
     8401 tcggacctcg atcgagaccc tgcgaaggtg cctgcgaatg cgaccagggt gaatgtgccg
     8461 gccgtggtga aaatcccttg cacgccgagt gatcgaaccg ccgcggtctg gatccccatg
     8521 gcgagcgaga acagggcgat gagcacgtcg acaatcccgg tcgaggggtg ttcggccgtt
     8581 gacaaccacg ccacaaggaa gctggcctcg gcaacggcca cgaggaccaa caggacggtc
     8641 accgccgggg accacaaacc agattcgttg gagcgcagcg tcgtaaaccg caggccgaag
     8701 taggccccca cggtgaacat cgatagcgcg acgatgacgg gtacgacatc gggcccctca
     8761 atctccgcga taccgaaccc caggaatacg aggttcccgg tcatgaacgc cgagaagatc
     8821 ttgcctagac cgaaatagct gatggcgtct accgcacccg aagcgactgt caacgctacg
     8881 agcagcagat cgcgcacgga ttggcgatct tgactgccgg cggtggtcga cgcggacaga
     8941 atgtctaatc tgatcggcgc ttcaggcgac atgtccccgc cgtgggattt tcttgccatg
     9001 acagcagctt gtcgtctcgg cgtcgaaatg tcgtcaccgc tgtcccacat ccgcaggccc
     9061 ctgtcagccg gagcaccccg tacctgccag cgtgagtgac aaacgctggc tgacacggac
     9121 gcgactctgc gctcctcccg aaaaatattt tgccgcaaag caactcacta gggcgcatag
     9181 caccattcct cggcgacagt tgcccggatg aagcgcctgg agccacgaga acaccctaag
     9241 gaattcacgc tgacgggcag acgaggttcc ggctaacggg cgggctcgaa gtcgtgtcag
     9301 cggtgatggc atcggtttgg agcaacggga ttctgaaagt acccagcggg gcggagcagc
     9361 ccgtgcctgg atcgggcccg cttgggcgcc cagcttcaaa cctgaaaccg ccccaaccgg
     9421 cagcgagagg aaccgatgac ggtcgacaac cattctcgcg cacgtctgga acctgtcgcg
     9481 gtgaaggtgc ccgagatagc cgcgtcgttc tgggttctca agctgctgac gaccgcgatg
     9541 ggtgaagcgg cgtcggacta tctgctcacc acgatgaacc tcatcggcct cggtgttggc
     9601 ctagcaggtt tcgcactcgc cctgtgggtt cagcttcgga caaggcggta caacgccttc
     9661 gcctactggg cggcggtgat gatgatcgcc gtgttcggca ccatggccgc cgacaccctc
     9721 caccaccaac tcggggtctc gtttgggctt tccaccctgg tctgcgcgct tgccgtcgcg
     9781 gcgaccttct gggcgtggta tcgcgttgag cacacactgt ccatacacag catcaccaca
     9841 cgacgccgtg aggtgttcta ttggctgaca gtgtcgttca cgtttgccct cggaaccgcc
     9901 gtcggcgacc ttacagcaag ctcactgcac tttggattcg tcggatcgat cgtcctgttc
     9961 gctgtcgtca tggccgttcc ggctttgggc tattggcgtt ttcgcctcaa cagcgtcatt
    10021 gcgttctggt gggcatatat cgtcacccgg ccactcggtg cctctgttgc ggattggttg
    10081 agtaagccgg ccaagtccgg cggcctcgcc tacggcgacg gccctgtcgc cgccatgctg
    10141 ctggcgttcg cgcttatgct ggtgaccgtt gcagccgtca gtcggcgagc tcctacattc
    10201 gaagcatgac cgtgccgttc tcctggcaac tgccgtacgc gtggccgagg acacccgttc
    10261 tcgcgcggaa cgtcgtctgc acatcgcaac cgctcgccgc gcaggcgggc ctggcgatgc
    10321 tggccgaagg cggtagcgcg attgatgcgg ccctggctgc cgcggtcaca ctgacgctgg
    10381 tcgagccagt gtcgaacggc atcggctcgg acgcgtttgc aatcgtctgg gacggaaggc
    10441 aattgcacgg gctgaatgcc tccggccgat cacccgccgc atggacgcag gaatacttcc
    10501 agggccgccc cgtgccggca cggggatgga actcggtgac ggtgccgggc gcggtgtcgg
    10561 cgtgggtgga gctgcacgcc aagttcggca aacgtccgtt cgcacggatc ttcgagccgg
    10621 cgatcggcta cggccgcgac ggattcctcg tgtcgccgac gatcgcctcg cagtgggcgg
    10681 cgcaggtgcc ggatctgcgc tcgcagcccg gcttcggcga ggcgttcatg ccgggcagtc
    10741 gggcgccctc gcctggggag ctgtttcgcc ttccagagca cgccgcgaca cttgagaaga
    10801 tcgccgcgac gaacggcgaa gcgttctacc ggggcgagct cgccgagacg atggcggcac
    10861 actcggccgc gaacggtggg gccatgatcg ccgccgacct cgccgcgcac cgcgccgact
    10921 gggtcggcac gatcaccacc gactaccggg gctacacgat tcacgagctc cggccgaacg
    10981 gtcagggcat cgtggcgttg gtggcgctgg gcattctcga gcacttcgac atggcttcgc
    11041 tcccagtcga ttcggcggac agcgtgcacc tgcagatcga ggcgctgaag ctcgcgtttg
    11101 ccgacgcgct tgcctacgtc gccgacatcg atcacatgca ggttcggccg ggacaacttc
    11161 ttcacaagga atacctgaag cagcgctcga cgctgatcga ccggacccag gcgcaagatg
    11221 tgacagcggg agctccaccc aagggcggca ccgtatacct caccgctgcc gacgcttccg
    11281 ggatgatggt ttcgatgatc cagtccaact acatgggctt cggctcgggt gtggtggtgc
    11341 ccggcaccgg aatatcgctg cagaaccgcg gcacgggctt tgtcacgaca cccgggcacc
    11401 ctaacctggt cggcccgaac aaacggccgt accacaccat cattccgggc ttcatgacca
    11461 aggacggcgc acccgtgatg agcttcggtg tgatgggcgg aaccatgcaa ccgcaggggc
    11521 atctgcaagt ggtagtgcgc atcgccgact acggccaaaa cccgcaggcg gcatgcgacg
    11581 gcccgcgctt ccgctgggta cagggcatgc aagtgagcgt cgaggggggc ttcccgccgg
    11641 cgacgctgga ggggctgcgc cgtcggggcc acgacatcgt cacggtcgat gactacaacc
    11701 aattcggcag ctgccaggca atctggcggc tcgatgacgg ttacctcgcg gccagcgatc
    11761 cgcgccgcga cggtcaggcc gcaggcctct aatttgggcc gcaaatcgac tgtcaaacct
    11821 ggcggtacta cgaacgtttc cctcggaccg ggagccaccc ttccgatccg gctgtacagc
    11881 caggttccgg ctcagcacac ctgtatcgcc tgggcggaca cccatcggtg attgccgcta
    11941 gctggggtcc ttggcttacc cgcctatcgc gttagacgcc gtgcagattg aagctagcca
    12001 cggcgtcatt gggcctgcga agctgaagga tcagttctcg cgcgtcggct ggaccgtcaa
    12061 atgcagcggc gcgtcgaccg gctggaactc gtaggaaggg catccggtga cgaacggcaa
    12121 gacgcatatt tcgggtggcc tgctcgggca gggtgaggct ggacgtcccg cggcggggcg
    12181 gggtccgaac gccggtggtc agttctccca agaggattac caccatcccg ctgcgggctg
    12241 gggcgcggcg cgcagcgtgg ccaaggtcct ggcgcacagc cgcgaactgg ttgccggcac
    12301 ccgggcaatc cagctgatga accacgagaa cggcggcttc gactgcccgg gatgcgcatg
    12361 gccggatgac accaagggtc tacacctgga tatctgcgag aacggcatca agcacgtcac
    12421 ctgggagatg acccgcaaga gggtcgatcg tgaattcttc gccagccaca ccgtgtctga
    12481 cctgtcgggg tggacggatt tcgcgctcga ggaccagggc cgactgaccg aaccgatggt
    12541 ctatgatgcc gagtcggaca ggtacgtgcc gatctcgtgg ccggacgcgt tcgggctggt
    12601 gggctccacg ctgcggaggc tggacagtcc acatcaggcg tcgttctaca cgtccggtcg
    12661 gctcagcaac gaggcgacgt ttctgtacca gctgtgggtg cgggagttcg gaacaaacaa
    12721 cctgcccgac tgctcgaaca tgtgccacga ggccagcggc cgagcgatga ccgcgtcgct
    12781 ggggacgggt aagggcacct gtgacctcga cgactgggag aacgcggacc tgctgctggt
    12841 gatgggggtc aacgccgcat ccaatgcgcc tcggatgttg acctcgctgg ccgaggccca
    12901 tcgccgtggc gcgcagatcg tgcatgtcaa cccgctgatc gaggctgcca gtcgccgcac
    12961 catcgtgccg cacgagatcg tcgcgatggc ggcatttcac gccaccaaga tcggaacgat
    13021 gaacgttcag ccccgggtcg gtggcgactt ggcgttgctg cgcggggtcg cgaaggtggt
    13081 tctcgagcgc gcgcagagcg accccaaagc cattgaccgg gagttcgtgg cgcgctacac
    13141 aacgggtttc gaggcctacc ggtcgctcgt cgagtcgacg ggctggtcgg agatcgtgca
    13201 gcagtccggt gtcgaggaag cgacgatccg acggctggct gaagtctatc tgggtgccga
    13261 ccggaccgtg gtggcctggt gtctggggtt gacgcagcag gagcacgggg tcgacaccgt
    13321 gcgcgagatc gtgaatctgc ttctgctgcg cggcaatatc ggccgtgaag gggccggccc
    13381 gtcaccggtg cgtggccaca gcaacgtcca gggcaaccgc acctgcggta tcgatcaccg
    13441 cccgcccaag caactgctgg atcggctcga agaggtgtgc gcgatcaccc caccgcgtga
    13501 gcacggcctg gacaccgtcg gcaccatcaa ggcgatgctc gccggcgacg taaaggtgtt
    13561 cgtcagcatg ggcggcaatt tcagcctcgc cacgccggac accgcggcaa cgttcgcagc
    13621 gctgcgcaac tgcgagctca ccgttcaggt cagcaccaag ctcaaccgca gccatctggt
    13681 gcacgggcgc aaggcgctga tccttccctg cctcggccgc accgagaagg atgttcagcg
    13741 gaccggcgag cagggcgtca cggtcgagga ctcgatgagc atggtgcact tgtcgtttgg
    13801 gatgaaaagc cctgcgtcgc cgcgtctgaa gtcggagtgc gggatcctgg ccggcatggc
    13861 ccgggcgacg ctgccggcct cggctacgcc ttgggagtat tacgtcgagg attacgaccg
    13921 tatccgcgac acaatggcca aggtgttcga cggcttcgag gacttcaacc gtcgtgttcg
    13981 cctaccgctg ggatttcgca tccggcagcc ggcaagggag agggtgttcc tcaccgcatc
    14041 cggatccgcg gagttctcag ccgcaccgat gcccgatgtc gtgccgccac ccggacgatt
    14101 gacgctgagc accgtgcgct cgcacgatca gtggaacacc accatctact ccgacgacga
    14161 ccgctatcgc ggcctcaaga acctgcgaac ggcgttgttc atgaatgccg acgacatgcg
    14221 cgaacacggg cttgacgagt tcgatctcgt cgacatcacc agcttcgccc gcgacggcag
    14281 cacccgctcg gtatacgggt accgcgccgt ggcttatgac accccgcgcg gcagtgtatt
    14341 cggctacatg cccgagttga acctgctatg cgccctcgcc gaccacagtg agcagagcgg
    14401 gcaaccgttg accaagcagc tcatcgtcga agtcagtcca gcgacggcgg gtccggccac
    14461 cactccaacg ggtgcacccg cggctggccg gtcctgatcg gtgtccactg gatcgtgtca
    14521 cgggtcgggg acccgaccag tccgccggtt cttgcggatt cagccccgcc gagccgcccg
    14581 gtcgtcgcct accgtccgac gcgacagccg ccgcctcaaa gcaggaatgc ttctccgccg
    14641 taggggtagt agttgacggg tggaggggcg tcgttgatct gcgcattgcc tggtgactgg
    14701 cagatggttc ccgatccatc cgcggagcac gactgcccgg tatcggccgc cgcaattggg
    14761 gcggcgagga tcgctaccgc ggcagctgcc gttccgaaca gcggtgtgat gtaggtcagt
    14821 ctgatgcgca ttggtgtctt ctttcgatga atggtcccga cctggctagt ccaacgatgg
    14881 cctttgatcg agcgcttgtc gcccctgctg acccccatcg acgtacctac tgactggaac
    14941 atcttcgtat gggctagcag ccggcaccca agcgaattga ccgccttgcc acacagcggt
    15001 tctcatgaac tctggccctg atccgcactg cggtcatgtc gagcagcgtc atcgaagagt
    15061 gacttaagcg ttgcggtgag gtgtcggcgg ttgacgatga atccagcgat ggccaacgcg
    15121 gcatacacgc cgcacaggat cagccgcccc gcggtggagg tgattgggaa ctgcacggcg
    15181 aaaagtgcca gcagcgccca ggccgtatag cggtggaacc gcagcgccag gataagagcc
    15241 acccccatca tcgtttgcgt cgcggtcagc aggacttcct cgatctggcg gccgtcgagc
    15301 tgcaagacgg tcccaccgcc gccgatcatg taggccaccg gcaacgaccc caccagcaat
    15361 gtccactggt tgaccttcga tgaaatcagc atcgcaatcg cggccgtgcc cttgccgcgg
    15421 gcggcgaaca ggatcgcgat gacgaactcg ggggcctcgg aggcaagcgg tgccagccac
    15481 tgcaccagca ggaaccggtc gatgcctagt tcggtgccag cgccaatcag gctctcggcg
    15541 aacggttcgg cgctcagcag aatgaccgcg gcggccaccg cgaacaaggc gaccaccgtc
    15601 gtgcggcgga cacgatcggg caggccgccg atggccgcgg ccgtgccgat cagctctggc
    15661 tcctcgacgt cgccgcgggt cagtcggtag aggtagaagc cgaaccaccc caacagcgcc
    15721 acaccgatgg tgatgtcgat gtgcccgctg accggaatac cgaacgcgac gacgccagcg
    15781 atcaacagaa atccgagttc cacgcggttg cccggatcca actgcagcgc tgaggttttg
    15841 ccgttgttca gcttgcgcgc cacggcgagc gcgaccagca ccacgacggg ccagcccaga
    15901 cccatcagca gccggttcga tccggtcatg ttcgccgcgg cgtattggac gtagtcggga
    15961 gcatggccgg cggtgtaggc gtagtacaga tcgacggcgt actcgggcag caccgcaatg
    16021 agtgccagca ccgcgatcgc aagaccaccc gacacgtcga tctgggcggc ctcggccgcc
    16081 caggccagca ggaaactcgc cgacaccacc gccacaccgt aaacacacag cgcgagaacc
    16141 ggattcgggt ggagccccac aacacgcagc accaacgcgg gcatgatgaa cacggtggtg
    16201 atgagcgctg aacggatcag ggcggcgcgg ggatgaggcg cggtggatcg gtcggtgcgt
    16261 atcggcgtcg tgacggacat gggtgtcctt cgttcggcga ccggatggaa cgcacggcga
    16321 gactccggtc ggcaacagtg gtgtcgtccg aaggtctcgc tcgccggacc agaagtccgg
    16381 tggatgaccg ggcggcaaga ccgccagtgt gtcgaccatc cgctcgcgcc gcgtcgaggc
    16441 gacggtgagg aactactccc cttcgccgac gaccgtacac accgatccgc accggagcaa
    16501 tgtagggagt gcatcaaata aatacggccg gacaacggaa atttgctgct tattgtcccg
    16561 aaatctggaa gttcggttgt ccgaccttct ttctacggca aacctgacgg ttccggggtt
    16621 gcgccgccgt tcccgacatt aaaacgctga ctcggcagcc cggcagcagc accgcgcgtc
    16681 ggtcatctcc ggcgacatca gtacacccag atgtccgatc accagggcag ccatcccggc
    16741 caggcggcgg agaagccagc ccgccaagtt aggtgatcac acccacgggc gacttcgatt
    16801 gcaccgcggc gatcaatgcg tcggcgtcat tgatcacgtc gtcagctacc taagaccccg
    16861 gcacccgtga gaggcgctcc gggtggcgat gagttcgtcg gcgatgcgtt tggtcgtcgg
    16921 cgcgatggcg tcgacagacc caggccgctg tcaacgccac aagcagaagg tcgtgcaatt
    16981 ggtagtctct gatcgatccg gcttcgatgt gttgcacggc atttcgccaa atcaccggcg
    17041 gtcggagaac cgaatgtcca ggctgcgcag ataggtctgc agccccgcat cctgcaccgg
    17101 gatgaggtgc gcgggtgcac cggactcgtt gaagtgcgca tgccacagtc ctggtggcgt
    17161 gacgaacgcg ccgcccgcct cccaatcgac gcgggtcgga ttgacaatgt cgccgcgctc
    17221 gtccaaccgg ctgcccagca gcgtgtagca gccgggctct gcgtcgagga tcagatcgag
    17281 cgcgaccgac tggtggcggt gggggcgctg cacctgatcc ggtggcagca ggccgaacat
    17341 cgcccacagc acgtgggtga tggtcagcgt ctgttcctgc tcagcgttgg ccagcaggac
    17401 actgaccctg ctcttgtcgt ttgccccagg gcgcgacgcg atctcgttaa gccggcccac
    17461 cgcatcctcg cgccggaact tcgttgcgtt aaaacgtggt tcggcagcct ctgcgccgag
    17521 atgacgaagc agcggctcgt cgtgcaccca gtacagcgca gcgtcggagt gggcgtaaaa
    17581 ggtggcttgg ctacgggcgg gcagggtcag gaagtcaccc ttctcccact gcacaagtcg
    17641 accgttgacc gccgagaaac cccggccgct gatcacgtaa tacagctgcg aggtggcatt
    17701 cggatcggtg gcgatgtgtt cacccgcacg gattcgcacg aagttcgcca ccaaagccgg
    17761 gctggtcgct ggtccatgag cgattcccag ttcatgcgac aagtccaacg gaatgatctg
    17821 agtgggtccg tcggaataca actcgcggct gaatgccctg atgggaatct tcgaagtgtg
    17881 gccggacccg gtcgggttcg cggccttgga gtactcgaag taccgcgcat cctcggccca
    17941 gtcctcgaag cgcccttcga acccgtactc ggcgacattt gtcattggcg caggaatcgt
    18001 cggatccaac gcatcctcat cggttgcggg ttggacgaga tctgcagtca cggttcctcg
    18061 tttcgtcggg cgcgtcgctg agtggttacc ttcgtcggtt cagcgcaatg gcctcaagtc
    18121 agcattgcgc tcgaccggca gaaccacatc ggcgccagca agtaagtact gagtaccgct
    18181 ggcaggagcc atcaatgggc gtcagcggtg acgacgtgag gtcgctgagc gcggagtatg
    18241 gcggagagag cacgaatcgc gctggctaaa aaccgcagcg ccgaatccat gaggccaggt
    18301 atgaacgtcc ttctagtagg cacgatcgag ccgtcgctgg ccaaggccgt tggagcgaaa
    18361 tataaagcac tgcaactgcc taacgcggcc acccgtagac gctttctcgc caagcacgga
    18421 gggtcgatca ccgccatcgt cgacggtggg cctcctggag tggacgccaa tctgatggag
    18481 gcgctgccca acctcggcgc catcgtgcac cagggagccg gctacgacac catcgacatc
    18541 gacgcggcgc gccgactcgg catcggggtc agcaacactc ccgacgtgct caatgacact
    18601 gttgccgaca cagccattgg cctgatgctg gcgacgatgc gcggcttgtg tacggcagac
    18661 aactttgtcc gctccggccg ttggcctctc gacggcaatt atccgctggg gagagacctc
    18721 agcggttcac gagtcggtat tctcggtctc ggccgaattg gatcagccat cgcgcagcga
    18781 ctcgtcggct ttgattgctc catcgcctat cacagtcgcc gccaagtacc cggcaccccg
    18841 ttccgctacg tcacctcgcc ggtcgagctg gctgagtcag ttgacgtgct tgtggtcgcg
    18901 accgttggcg gcccaggaac caaacacctt gtgaaccgga cggtgttgga agctctcgga
    18961 ccgtacggat atgtcataaa cattgcccgc ggcagcgtcg ttgatcagga cgcgctagtt
    19021 gaactgcttg ttgcgcgccg gttggcgggc gccggactgg acgtcttcgc cgacgagcct
    19081 tatgtaccaa cggaattgtg cggattagac aatgccgtgc tgctaccgca catcggcggg
    19141 gccacagtcc gaagcgtctc gatgatgagg gagctggtgt tgcggaatct cgaccagtac
    19201 ctcaacaacg ggacgctgac aacgccggtc gtgccatcga ataggcgcgc acgtaacggg
    19261 attcacaagt gctctggttt caggccgtga ggccacaccg atagacaaca aggaggtggc
    19321 ggtcatggcc gctccgaccg aaacgccgat tcatgccgcg aaccagtacc gaccgccagc
    19381 ggtcagcggg ctgttcgacc tgctcgacca gcatgcgcac gatcgaccgg acgcacgggc
    19441 cttggtggcc ggaagcgatc gaacccccat ctcctatggc gaactcgccg ctctggtcaa
    19501 ggacgtggcc aatcggctgg cgaactcggg gctgcgccgg ggggatgcgg tgggcgttgt
    19561 cggcgccaac aatcccgact tcgtcgtcgc actacttggc gcaacgagag ccgggttggt
    19621 cgtcgctcct ctggatcccg cgctgccccg gtcagagatg accacacgcc tcgaaaggtt
    19681 gggagcccag gcgctgctcg tcggccgacc cgcactcgac gctgagccgg tagcaccctt
    19741 gggcatcccg acttggaatc tcgaagtaca cattgccgcg cacaggaccc cggccgtcac
    19801 actcgatgcg ggtgcactcc gacgctcgga gccgaaatct ggactctccg aacgtgacgc
    19861 gctcgtcctg ttcacatcgg gtaccaccgc ccaagcgaag atggtcccgc tgacgcacga
    19921 aaacgtggcc gcctcagtcc gcgcgatttg cgtctcatac gagctgggtc ccgacgatgc
    19981 gacggtcgca gtgatgccgc tgtttcatgg tcacgggctg atcgcggcgc tgctggcgtc
    20041 tctggccagc ggcggatgcg tgttgctgcc cgagcgtgga cggttctcgg cgcgttcgtt
    20101 ctggggcgac atgcgcgccg cgagcgccac gtggttcacc gcggttccca ccatccacca
    20161 gatcctcttg cagcggtcgg cgagcgatta tcccggcgcg aacgcggtcc ccttgaggtt
    20221 cgtgcgcagc tgcagcgccc cgctcaacac ggccatagcg cgggcgatgg agcgtacgtt
    20281 cggcgccccc gtcctgtccg cgtacgggat gaccgaaacc acgcaccaag cggccagcca
    20341 gaccgtccgc ggaaacgtga aagagggatc ggttggctcc gccaccgggg tgagcctccg
    20401 cgtcgtcggc cccgacgggc gtaggtgccc agccggcacc gaaggggaag tgtgggtgca
    20461 aggtcccacg gtcgcccgtg gttacctccg cgacccagcc gagaccgcgc acaacttcgt
    20521 cgacatgtgg ttccgcactg gtgatctggg cgcaatcgac gaagaaggat acctatttgt
    20581 gacggggcga atcaagaaca tcatcaaccg cggcggcgaa aagatctcgc ccgagcacgt
    20641 cgaggccgtc cttgcgcgct gtcgcggtgt cactgaggcg gccgtgttcg cgatccccga
    20701 cgcgacctac ggggaacgag tcggcgcagc ggtggtcgtg ccccaggacg aaagcttcag
    20761 ctcggaccag atcctgcaag agtgccgcgg cacgctggct gctttcgaga tgcccgaacg
    20821 gctcgaagtc gttgcggcct tgccccacac cgccaagggc gcgctcgaca ggcgcgcggt
    20881 agccgagcaa tacgtgcgat gtgcgcggtc agatgacgat ccgctctgtc ggcgccgtcc
    20941 gatcgaagaa ctcgtcgagg gctaagcctt ctgccgcgct gacgaatgca cgggccacca
    21001 ccgagcccgg tgtcgccgcg tgagtcgcca ccgagatctg cgcactcacg tccggctcga
    21061 ccagccggac ggcccttgcc ctgccgacca ccggcatcgc gcgcaaccag gtatgcggca
    21121 cgatgctcgc ccatgctccc acgccaacct gtgcgtacag ggaggcgacc gagtccgtct
    21181 ccacctgcgg ggtcaccgtg accccgcttt gcgcgaacgc gcagtcgatg acccggcgaa
    21241 tcctcatatc cggcgttaac aacacgagcg ggagttgggc ggcatccgcc cacgtcatgg
    21301 tggggtccgg cggaactggt aggtcgtcgg gtgctaacaa gatgtatcgc tcttcgtaca
    21361 gtggaaccgc tcgcaagcag gactgctcct cggagtcgaa atgagctatg gcagcgtcca
    21421 attcgaagtc aagcagctgg cggcacagtt cggtcgtcga caaccgagac tcgacctgca
    21481 ctttggccag tgggtgcgcc gcgcagaacg cggctacggg aagggtctgg gtggtcgaag
    21541 cagtcggttc tgtccccagc cgcagggtcc cggtgatgcc cgactggacg gcggccacct
    21601 ccgccttaaa cgcctcctgt tcggcgagaa tccgcttcgc ccagaccaca agccgttcac
    21661 cctccatcgt gagaccctca aaattgtggc cgcggttgat cagggtgacg ttcagctccc
    21721 gctcgagctt cgcgatcgac gccgagagcg cgggttgcga gacgtaacag acctcggccg
    21781 cccgggcgaa gtgtcgttct cgagcgaccg cgacgaagta ctccagttgt cggaacaaca
    21841 cgagcgacgg cctcccttgc aggttggtct acgggtcatc gttgcccctc agtgaacgaa
    21901 cgtcgccacc gctgtcggca atcaatgccc cctgctagct gcaaacttgc tcgatccgcg
    21961 ctgtgcgggc cgcctggccg ggcagtattg gtcaaggacc ggtccgccac gttcgggaca
    22021 gcacaagcct cgggccgggc agaaggtggt gagtcgagtg cgcgtggtag tggccgacga
    22081 cgacttgttg cttcgggagg gcttggccag cctgctggat cgctcggggt tcgaggtcgt
    22141 gggacaggcc ggtgacggtc gggagttgct cgcgatggtc cgcgagacca cgcccgattt
    22201 ggtggtggcc gatatccgaa tgccgcccac ccataccacc gagggactcg atgcggcgcg
    22261 ggtgatccgc gaggagttcc ctgacatcgc catcatggtg ctgtcggcgc acgtggatgt
    22321 cgaacatgcg atggaattgt tggccagcgg gcgcagcatc ggttacctgc tcaagaatcg
    22381 ggtaaccgat gtgacggatt ttttggacac acttgagcgc atcgccaacg gcgcgtcggt
    22441 ggttgatccc gcgttggtgt cggagttggt ctcggcgcgg cggcgcgatg atccactggc
    22501 ggcactgagt tcccgggagc gtgaggtgtt ggcgttgatg gccgaggggc gttccaatgc
    22561 cggtatcgcc catcggattt gggtgaccga aggcaccgtg gaaaagcatg tgcgcagcat
    22621 cttgaccaag ctggacctgg cggagaccgg cgacgatcac cgccgggtgt tagcggtgat
    22681 caccttcctc gagacgcgct gactctccta cgagatcgtg gatggcgccg cacgtcagag
    22741 cggacttgga caggaacccg acggctgggc tggtctcgat catgtccgtg tagtcctgcg
    22801 cggcgtgggt ggagatcagt atcaccgggg acggtgaggg cccccccgtc cggtggagtt
    22861 gctcggcgag ttcgaagccg ctctcgcccc cgaggttgat gtcgaccagc gtcacgtcgg
    22921 gccgcgattc ggcgaaaagc cgaagcgcgt cagcactagt cgacgccacg ccgacgaccg
    22981 tgatgccctc gcactccagc aatccgcacg cggcctccac gaaatccgca ctgtcgtcga
    23041 cgatcagaca gcgcatgggg tcactcgcag cagcatttaa tcagcttcgc agtgcgggcc
    23101 accgtccgca ttcccactac ctgcaaaatt ttgcgctcaa caacagcggt tgcagctagc
    23161 gggtacgtca aattccccct accagggggc aactgatggg tatcagcgtt gccgacacag
    23221 gctggcgcgc aggcaatcct ggaggacacc aacgcagacg cagatctgcc gatgctcgat
    23281 gagattggaa cagcatgaaa accacaacac tgggccgtag cggcctgaaa gtctcgcgca
    23341 tcgccttcgg cacctggcag ctcggcggcg actggggcga gttcgacgag aacgccgctg
    23401 tcgccgcgat ccgccgggcc cgcgagctgg gcgtcaactt cttcgatacc gcccaggcct
    23461 acggcttcgg agcgtccgag gccattctgg gtaaagcgtt gcgcgacgaa ttcagccgtg
    23521 accgcgacga gctcgtgatc gcaaccaaag gcggtctgcg ccaaactgat tccggcctcg
    23581 tccgcgatgc aagtccggaa tggctatgcc gaggtgtgga cgcaagcctc accgcgctgg
    23641 gtaccgacca catcgacctg taccaggtgc actggcccga ccccaccgtg ccggccgccg
    23701 agacggcggg cgcgctgagt gacatgatca ccgaaggaaa gatcggccat gtcggcgtgt
    23761 ccaattacaa ctccgcgcag atggccgagt tctccgcgac attgccggtt gaaactctgc
    23821 agccgccgta tcacctgttc cgccgtgaga tcgaggacga ggtcctgccg tactgccgcg
    23881 acaacaatgt cggtgtgctc gtctacgggc cgttggcaca cgggctgctg accggaacgc
    23941 tgagcgtgca caccgctttc gaggacggcg attggcgcgg caccagcaag gtcttcaggg
    24001 gcggcacgta ccgtcgcaac ctgggtgcgg tgcgtgagtt ggaaaagttc gccgccaacc
    24061 gcggcattac cgtcagccag ttggccatcg cgtggacgct ggcgaaccca gccgtgcacg
    24121 tcgcgatcgt cggtgcccgg caggtcggtc acatcgaaga cagcctccgt gccgtcgaca
    24181 tctctctcac cgactcggat ctcgatgaga tcgaattgat catggaagag gccacacccg
    24241 ttgctggacc gtccccggag ggcatgccgt gaccgggccc gcggtgaccg ttgccgtgct
    24301 gggcaccggg acgatgggcg ctccgatcgc ccgcaacctg cttcatgccg ggtttgacgt
    24361 gcgcgtgtgg aaccgcactg tggccaaggc tgccatggtg ggcgtcgatg cacaccgcac
    24421 gtccacccct ggggaagccg ccgccggcgc cgacgtgttg atcaccatgc tcgccgacgg
    24481 cgccgccgtc gagcaggtca tgaccggccc gacaggtgcg ctgtccaagc ttggggcggg
    24541 tgcgatatgg atccagatga gcacagtcgg tgccgaatgg accgaccgtc tcgccggcct
    24601 ggtggccagg catgacgtcg cgttcgtcga cgcgccggtc tctggtagct ccgccgccgc
    24661 ggacaagggc gaactggtca tcctcgcctc cggggcaata acgatccgtt cacgggtaga
    24721 gccaatcttc gacgtgctgg gacatcaaac gttgtggcta cagagcacag gtgacggcag
    24781 ccggctcaag c
//
LOCUS       DASRJA010000728         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1711994,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000728 DASRJA010000000
VERSION     DASRJA010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711994"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..136)
                     /locus_tag="VFC01_32960"
     tRNA            complement(64..136)
                     /locus_tag="VFC01_32960"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(101..103),aa:Arg,seq:acg)
     gene            complement(169..257)
                     /locus_tag="VFC01_32965"
     tRNA            complement(169..257)
                     /locus_tag="VFC01_32965"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Ser,seq:gct)
     gene            344..1393
                     /locus_tag="VFC01_32970"
     CDS             344..1393
                     /locus_tag="VFC01_32970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZN84467.1"
                     /translation="MTARLRPELADLPAYTPGKTVPGAIKIASNETVYGPLPSVRAAI
                     EKAADNINRYPDNGYVELKDRLAKHVDFAPEHISVGCGSVSLCQQLIQITSTVGDEVL
                     FGWRSFEIYPLQVRTAGATPVRVALTGYTYDLDAMLAAITDRTRLIFVCNPNNPTSTV
                     VDPDALTRFVEAVPSHILIALDEAYVEYIRDGLVPDSFGLVRKHSNVVVLRTFSKAYG
                     LAGLRIGYVVGDPDIITALGKVYVPFTATSISQAAAVASLDAADELLARTDAVVAERA
                     RVTAALREAGYTVPPSQANFVWLPLPGRAQEFANDSANARVIVRPYGDDGVRVTVAAP
                     HENDAFLAFAQRWAR"
     gene            complement(1390..1938)
                     /locus_tag="VFC01_32975"
     CDS             complement(1390..1938)
                     /locus_tag="VFC01_32975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HZN84468.1"
                     /translation="MPTDELESAEESLAVLEHVLHGIAKEDESKQTPCREFDVAKLTD
                     HLMNSITTIGGHAGAEFPERNRDDSVERQVILAARPALDAWQKRGIDGTVPFRDGEAP
                     AKMMAGVLSIEFLVHAWDYAKATGREVTAPDSLSDYVLGLAKTIISPQGRTSVGFDDP
                     VDVAADARALERLIAYTGRQPN"
     gene            1979..2416
                     /locus_tag="VFC01_32980"
     CDS             1979..2416
                     /locus_tag="VFC01_32980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84469.1"
                     /translation="MRTRVWVAGAALILAGLTAGCERTTPGTVAMTTQPGPPIASSPG
                     TPSTVPGLPGIPIPDIQIPGFPRNTDVPVVPAPPNALTMTCGEFNKLDEATRLAVIRE
                     ILAQENNPLGPNGESVGQMLVEAACQFLPEATVSEVLMGTAPR"
     gene            2436..>2859
                     /locus_tag="VFC01_32985"
     CDS             2436..>2859
                     /locus_tag="VFC01_32985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN84470.1"
                     /translation="MGDAYESVTVDIEGHVAQVTLVGPGKGNAMGPAFWAEMPEVFAK
                     LDADHDVRAIVLTGSGKNFSYGLDLPAMGGSLSGVLGGGGSARPRADFHQTILRMQGA
                     INAVADCRTPTIASVHGWCIGGGVDLISAVDIRYASADA"
BASE COUNT          484 a          982 c          910 g          483 t
ORIGIN      
        1 cgactgcaga aacgacatag gctcggaccc ccaacagtgc aggtcaaagc ctatgaatcg
       61 tagcgcgccc gaagagattc gaactcccaa ccttctgatc cgtagtcaga tgctctatcc
      121 gttgagctac gggcgcatgt cttcaattgt gagccgacga gtcagccgcg gaggcgagag
      181 gatttgaacc tccggtcgcc tttgagggcg acaactcatt agcagtgagc cccattcggc
      241 cgctctggca cgcctcctcc ggcgttttac cgccgatccg acagagtaca cagaggcatt
      301 aggcgaaggc aaagcgcagg tgagcgaccc ataaactggt cgtgtgaccg cccgtctgcg
      361 ccccgagcta gccgatctgc ccgcatacac cccaggcaag acggtgccgg gcgcaatcaa
      421 gatcgccagc aacgagactg tgtacggacc cctgccgagc gtccgggccg cgatcgagaa
      481 ggccgccgac aacatcaacc gctatccgga caacgggtac gtcgagctca aggaccggct
      541 agccaagcac gtcgacttcg cacccgagca catctccgtg gggtgcgggt cggtcagcct
      601 gtgccagcag ctcatccaga tcacctcgac cgtcggcgac gaggtgctgt tcggctggcg
      661 cagcttcgag atctacccgc tgcaggtgcg caccgccggc gccacgccgg tgcgtgtggc
      721 gctgaccggc tacacctacg acctcgacgc gatgctcgcc gcgatcaccg accgcacccg
      781 gctgatcttc gtgtgcaacc ccaacaaccc gacgagcacc gtcgtcgacc ccgacgcgct
      841 gacccgcttc gtcgaggccg tcccatcgca cattctgatc gcgctcgatg aggcctacgt
      901 cgagtacatc cgcgacgggt tggtccccga cagcttcgga ctggttcgca agcacagcaa
      961 tgtcgttgtg ctgcggacgt tttcgaaggc gtacggcctt gccggcctgc ggatcgggta
     1021 tgtggtcggc gatccggaca tcatcaccgc gctgggcaag gtgtacgtgc cattcacggc
     1081 gacgagcatc tcgcaggccg cggccgtcgc gtcgcttgac gccgccgacg agctgctggc
     1141 ccgcaccgac gccgtcgtcg ccgaacgtgc ccgcgtcact gccgcattgc gcgaggccgg
     1201 atacaccgtg ccgccgtcac aggccaactt cgtctggctg ccgctgcccg gccgtgccca
     1261 ggagttcgcg aatgactcgg cgaacgcccg cgtcatcgtc cgtccttacg gcgacgacgg
     1321 cgtgcgggtc accgtcgccg cgccccacga gaacgacgcg ttcctggcgt tcgcccagcg
     1381 ctgggctcgc tagttcggct ggcggccggt gtaggcgatc agtcgctcca gcgcgcgggc
     1441 gtcggcggcc acatcgaccg gatcgtcgaa gccaacgctg gtgcggccct gcgggctgat
     1501 gatcgttttc gccagaccca gcacgtagtc ggacaacgag tcgggagccg tcacctcgcg
     1561 gccggtcgcc ttcgcgtaat cccaagcgtg gaccaggaat tcgatcgaca acacgccggc
     1621 catcatcttc gcgggcgcct cgccatccct gaacggcacc gtgccgtcga tgccacgttt
     1681 ctgccaggcg tctagcgcgg gccgcgccgc gagaatcact tgccgttcca ccgaatcgtc
     1741 gcgattcctc tcggggaact cggccccggc gtggccgccg atcgtcgtga tcgagttcat
     1801 caagtggtcg gtcagcttgg ctacgtcgaa ttcacggcac ggtgtctgct tcgattcgtc
     1861 ctctttcgcg atgccgtgca gcacgtgctc cagcacagcc agcgactcct ctgcactttc
     1921 aagttcatcg gtgggcatgc gcctacgcta cgactctctt gcgcctatcg ttcacgggat
     1981 gaggacgcgc gtatgggtgg ccggggccgc attgattctt gccggcctga ccgccggctg
     2041 cgagcgcacc acacccggca cggtggcgat gaccacccag ccgggcccgc cgatcgcgtc
     2101 cagcccagga acgccgtcga cggtgccggg gctgcccggc atcccgatac cggacattca
     2161 gattcccggg ttccccagga acaccgacgt tccggtggta ccggcgccac cgaacgcgct
     2221 gaccatgaca tgcggcgagt tcaacaagct cgacgaggcc acccggctgg ctgtgatccg
     2281 cgagatcctg gctcaggaga acaatccgct gggtcccaac ggcgagtccg tcggacagat
     2341 gctggtcgaa gctgcctgcc agttcctgcc cgaagcaacg gtcagcgagg tgctgatggg
     2401 aacggcgcct cgctgactgg ctaaccttgc tggccatggg cgatgcatat gaatccgtaa
     2461 ccgtggacat cgagggtcac gtcgcacagg tgacgcttgt cggtcccggc aagggcaacg
     2521 cgatggggcc ggcgttctgg gcggagatgc ccgaggtgtt cgccaagctc gacgccgacc
     2581 acgacgtgcg cgcgatcgtg ctgaccggct ccggcaagaa cttcagttac gggctggacc
     2641 tgccggcgat gggcggctcg ctgtcgggcg tgctcggcgg cggcggatcg gcccgtccgc
     2701 gggcggactt tcaccagacg atcctgcgga tgcagggcgc gatcaacgcc gtggccgact
     2761 gccgcacccc gaccatcgcg tcggtgcacg gctggtgcat cggcggcggc gtggatctga
     2821 tctcggccgt cgacatccgg tatgccagcg cggacgcga
//
LOCUS       DASRJA010000729         4293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1335092,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000729 DASRJA010000000
VERSION     DASRJA010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335092"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFC01_32990"
     CDS             complement(<1..438)
                     /locus_tag="VFC01_32990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84471.1"
                     /translation="MANIKNRPGVASPSRQNNVLPLAGHAKFSQPPSRKPARPRTGGV
                     TPTGLEEWTARGRTLYSTLNRTQWEIGAWFNGGHRYGQRKAVVTAIGFDYHTAKNYGW
                     VERKVERSRRRDLSFTHHEKVAALLPDDQVRWLDIAVRDKLSTT"
     gene            complement(445..672)
                     /locus_tag="VFC01_32995"
     CDS             complement(445..672)
                     /locus_tag="VFC01_32995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84472.1"
                     /translation="MPGEDDQLLTPSETASWLAVSPQWLSIGRHRGWGPPFERLTPKV
                     VRYRRGSVRAWLAEREHRRTADYAASDSVSA"
     gene            complement(1085..2299)
                     /locus_tag="VFC01_33000"
     CDS             complement(1085..2299)
                     /locus_tag="VFC01_33000"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HZN84473.1"
                     /translation="MTKLTEKSVAKLRAPDPTGKQKLHWDTELRGFGVLVSGVSTSKS
                     YVVQHALRDGRKRRVTIGPVNVVSITEARERAREILGDFYQGRDPKVVRRGDPTLASA
                     LDTYLETRTDLRPRSAESYRDLIERHLSAWLDTPLRDITRDVVEDRLKKIARDVAADS
                     GTSGHATANGTMRALRAVYNFAADRAPPSDPLPQNPVRLKKVWFPVKKRTGHLTPEQL
                     PEFYAAVDALQNRVGADYLKLILFTGLRRREAAALKWQNVDLKKAVLSLPASSMKGKR
                     PLDLPLTDFVRDLLIARRTIGADKWVFPSASASGHVKEPRFFLDATGFDVTVHDLRRT
                     FVTVAEGCDLSHYALKALVNHALADDDVTSGYIQMTPERLREPAQKVCDRLMQLCKIK
                     GPTGANVAKLKK"
     gene            complement(2569..3159)
                     /locus_tag="VFC01_33005"
     CDS             complement(2569..3159)
                     /locus_tag="VFC01_33005"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZN84474.1"
                     /translation="MTIFGYARVSTDGQTLAAQDAQLHAAGCAKVYAEKASGAKTDRP
                     ELAKVLRRLEAGDVLVVTRLDRLARSTRDLLNILDTVGKVGAGFKSLGDTWADTTTPH
                     GGLMLTVLGGLAEFERGLILARTSDARRRAMAKGVKFGRPPALTAHQRKEALQRLANG
                     ESHADVARSFAVSRTTISRLVPSPFASEATSAGAVV"
     gene            complement(3851..>4293)
                     /locus_tag="VFC01_33010"
     CDS             complement(3851..>4293)
                     /locus_tag="VFC01_33010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84475.1"
                     /translation="AVRSTAANDVDLDMIFAAIEAHRRAEAEFSAALGRLSDLENELP
                     EERRRSEINMGVLAIVSKDDSRWIEIQRTVMATCKITDAAATNLLNTRPTTLAGVVAV
                     LRYAYDFERRGLEFPGGYDDGDLSECPWGVQWSVYFHRNLAQAI"
BASE COUNT          711 a         1366 c         1349 g          867 t
ORIGIN      
        1 ggtcgtgctc aacttgtcgc gcaccgcaat gtccagccaa cggacctgat cgtccggcag
       61 gagcgcggcg actttctcgt ggtgggtaaa tgaaaggtcc cggcgccggg acctttcgac
      121 cttgcgttcg acccagccgt aatttttggc cgtgtgatag tcgaagccga tagccgtcac
      181 cacggctttt ctttgcccgt agcgatgacc cccattgaac caggcaccga tctcccattg
      241 ggtacggttt aacgtcgaat agagggtgcg accgcgagct gtccattcct ccagcccggt
      301 cggcgttacg cctccggtgc gcggacgtgc tggctttcgg ctaggtggct ggctaaactt
      361 cgcgtgtccc gccaggggca acacgttgtt ctgccggctc ggggatgcca ccccgggccg
      421 gttctttatg tttgccatgg ccggtcacgc tgacaccgaa tcggaagcgg cgtagtcggc
      481 ggtgcggcgg tgctcgcgtt cagccagcca ggcccgaacg ctgccgcgtc gatagcggac
      541 gaccttcggc gtcagacgtt cgaatggcgg accccatccc cggtgccgac cgatgctcag
      601 ccactgcggt gacaccgcca gccaggaggc ggtttcgctg ggggtcagca gctgatcgtc
      661 ttcacctggc acggaggcaa ggacgtgggc tcggcgatca aggtgatgcc ggtttgggat
      721 tggagcttgg aatttgggag ccggtggtcg cttggacttg gctgaagcca tggtcggtcc
      781 tcacccacga gccaacaccc cacgcagacg cgtggttgct gtgctcggtg tgccgttagc
      841 gacggcggag tgaggccagc gaccctaccc gtagggtccg gtcatccgag gcgttgtcct
      901 cgggtctcgg gtccccgcaa tcctgtggcg ttgtctgcgc caccatgcgg acggcccttt
      961 aggcggagtt cggtgtgcca tgctacaggc accgaccgtc aggcttatct ccgcctgtcg
     1021 cctgacgtgt cgtcattggt gtaacttata aactgtgccc ggagatgatt tccagtccct
     1081 ctttttattt tttcaacttc gcaacattgg cacccgtcgg tcccttgatc ttgcacagct
     1141 gcatcagccg gtcgcacacc ttctgtgccg gttcgcgcag ccgctccggt gtcatctgga
     1201 tgtagccgct cgtcacgtcg tcgtccgcca gcgcatggtt gaccagtgcc ttgagtgcgt
     1261 aatggctcaa gtcgcaacct tccgcgacgg tgacgaatgt tcgccgcaga tcgtgtacgg
     1321 tgacgtcaaa gccggtcgcg tccaagaaga acctcggctc cttgacgtgc cccgacgcgc
     1381 tggccgacgg gaacacccac ttgtcggcgc cgatggtgcg gcgagcgatc agcaagtcac
     1441 gtacgaagtc ggtgagcggc aagtccaacg gccgtttgcc cttcatgctc gacgcgggca
     1501 ggctgaggac ggccttcttc aagtcgacgt tctgccactt gagcgccgcc gcctcccgtc
     1561 gccgcaggcc ggtgaacaag atcaatttca ggtaatcggc accgacccgg ttctgcaacg
     1621 catcaactgc ggcgtagaac tcgggcagct gctccggtgt caggtgcccg gtccgcttct
     1681 tcaccgggaa ccacaccttc ttcagccgca cagggttctg ggggagggga tcggacggtg
     1741 gtgcccggtc ggcggcaaaa ttgtacaccg cacgcaaggc ccgcatcgtg ccgttggcgg
     1801 tggcgtgccc cgaggtgccg ctgtcggccg caacgtcccg ggcgatcttc ttcagccggt
     1861 cctcgaccac atcgcgggtg atgtcgcgca acggggtgtc cagccatgcg gacaggtgcc
     1921 gctcgatcag gtcgcggtaa ctctccgccg atcgcggtcg caggtcggtt cgggtttcca
     1981 ggtacgtgtc gagcgcagac gccagcgtgg gatcgccgcg ccgcaccacc ttggggtcac
     2041 gcccctgata aaagtcgccg aggatttccc tggcccgctc cctggcctcg gtgatcgaca
     2101 cgacgttgac cgggccgatg gtgaccctcc gcttgcgacc gtcgcgcagc gcgtgctgca
     2161 cgacgtacga cttgctggtg ctcacaccgg agaccaggac accaaacccg cgcagctcgg
     2221 tgtcccagtg cagcttctgc ttcccggtag ggtcgggtgc cctgagcttg gcgacggatt
     2281 tctcggtcaa cttggtcatg actaaagctc ccccagttcg cgttgcatgc tgcgaacctc
     2341 gccgtactgc cgccgagcgg tgattgggtt gacgccgacc agggcagcgg cggcgacgaa
     2401 ttcgttggcg gtcatcgtca ccgggcagta catcactgcg gtgcgcaggt taggtcacca
     2461 gctcgcggct aatgcgctgc accgtgccgc tgccaacacc atgcagctcg gcagtcttga
     2521 gcaggatggc ggtgtgcgtc cggcgaacct cggcccgcgc ctatcaaact ataccacggc
     2581 gcctgcgctt gtggcctcgc tggcgaaagg gctggggaca agccgcgaga tcgtcgtccg
     2641 cgagacagcg aagctccgcg ccacgtcagc atgtgattcg ccattggcaa gccgctgcag
     2701 ggcttccttt cgctggtggg ccgtcagggc cgggggccta ccgaatttca cgcccttggc
     2761 catagcgcgg cgcctggcgt cgcttgtacg ggcgaggatg agcccgcgct caaactcggc
     2821 cagcccgccg agcaccgtca gcatcaggcc gccgtgcggc gtggttgtgt cggcccaggt
     2881 gtcgcccagc gacttaaagc cggcgcccac cttgccgacg gtgtcgagga tgttgagcag
     2941 gtcgcgggtg gaccgcgcca ggcggtcgag gcgtgtcacc accagcacgt cgcccgcctc
     3001 gagtcgccgc agcaccttgg ccagttcggg acggtcagtc ttggcgcccg aggccttctc
     3061 cgcgtagacc ttggcgcagc cggcggcgtg tagctgggcg tcctgtgccg ccaaggtttg
     3121 cccgtcggtg ctcacccgcg catagccgaa aatcgtcatt tcgccctccc acttttgcaa
     3181 atagcggcag ccacaatcga gtcaatttga attttttgga caatttacac ctggactagc
     3241 cgttgaattc ttcgcagcgc accgaaggct agggcccggc ggcggtatgg gcaaagaagg
     3301 gcaaaccctt atcaggtgaa ttaccgattt ttggctgtag tttgtgtgtt agtctctcaa
     3361 gcctcaagct cgggaggcgt gccatgttcg aatgcgctat ttgcgtggcc gtttttacac
     3421 ttgtcgtcgt tgcgatagtc ggcgtaagcg ccgtgatggg ataggaagaa ggctcgtact
     3481 ggcgaatcga caacaccagc aggtaagctc gggtcactac gtcccatcgc aggttccacc
     3541 tccagtcggt tagcgcggaa atcgacctct acagacaggt gacctgcggt tccctgcgat
     3601 ggcctagcct aagcgggctg gggtttaagc atgccgtcca aggtcaccga tccgcgcccc
     3661 gcacccgctg cggccacgcc tcccgccgtg cctggcgctc tctgtaggca ggcctgctcc
     3721 cgtggcaggc cgcagccgct cgggctgctc agtgccgggg tttcgcctgc tgcggcctgt
     3781 tggcaggcgg gcgaccaacg ttcgccattt agctttagga ttgagacggg cgctgtccga
     3841 caatcatagc ctagatcgcc tgggcgagat tgcggtggaa atagaccgac cactgcactc
     3901 cccaaggaca ttcgctcaaa tcgccgtcgt cgtagccgcc ggggaattcc aaaccgcgcc
     3961 gttcgaaatc gtaagcgtac cgcaagactg ccaccacacc agcaagcgtc gttgggcgag
     4021 tattgagaag attggtcgcg gctgcgtccg tgatcttgca agttgccatc acggtcctct
     4081 ggatctcgat ccaccgggag tcatccttcg aaacaattgc gaggacgccc atgttgattt
     4141 cacttcgacg ccgctcctcg ggaagctcat tctctaggtc ggaaagccgt cccaaggcag
     4201 ccgagaactc ggcttccgcc ctgcggtggg cctcgatggc agcaaagatc atatccaaat
     4261 ccacgtcgtt cgcggctgtg cttctcacgg cgt
//
LOCUS       DASRJA010000730         3383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1230947,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000730 DASRJA010000000
VERSION     DASRJA010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230947"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFC01_33015"
     CDS             <1..257
                     /locus_tag="VFC01_33015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN84476.1"
                     /translation="NDGYNWKGDPNATWTGQDIPQQPPGSPPTAAPPAAVPAPIAVAP
                     YDPATGTYVGPDGKVYTQSDLANTAPEEKTWQTMLIPPGS"
     gene            224..844
                     /locus_tag="VFC01_33020"
     CDS             224..844
                     /locus_tag="VFC01_33020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mammalian cell entry protein"
                     /protein_id="HZN84477.1"
                     /translation="MADNADPAGELTLLLDDDDAVDIEDDGAGCSADDADAAPGPSAH
                     EPISAARRAMKLALAVAVALAVLVGWLGFRAHQSEQVHAQRSQFLQAARQCALNLTTI
                     DWQKAESDVHRILDGATGQFHDDFAKRAEPFIQVVKQAKSTTVGTVTDAGLESQTADS
                     AQVLVAVTVTTSNTGAPQPDPHSWRMRISVQEVGDQAKVSNVEFVA"
     gene            841..1497
                     /locus_tag="VFC01_33025"
     CDS             841..1497
                     /locus_tag="VFC01_33025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84478.1"
                     /translation="MRLLTSRRAEDPTDTDLVDDAAAELEFVTGQDETEVTERATDAD
                     GSDHDDQTRIARRRIRWSKAIAYGLIPGLALLLASAAGYLKWQDGTTREAQAAGAESV
                     RSAIDSTVALLSYRPDTVEKDLDAAKGRLTGTFLNAYTSLTRDVVIPGAKQKQVSAVA
                     TVPAAASSSATPTHAVVLLFVDQTVTVGQSAPTNTASSVRVTLDKVDGRWLISQFDPV
                     "
     gene            1548..1901
                     /locus_tag="VFC01_33030"
     CDS             1548..1901
                     /locus_tag="VFC01_33030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HZN84479.1"
                     /translation="MGALFATAATLSAATLGLAPNARADVVAYLVNVHVRPGYNFPNA
                     DAALGYGYGICDKVAAGRGFPDVMADVRADFNTSDDYQASYLISQAVNELCPAQIWQV
                     RNSAAHYQSPPGVTP"
     gene            1918..2607
                     /locus_tag="VFC01_33035"
     CDS             1918..2607
                     /locus_tag="VFC01_33035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZN84480.1"
                     /translation="MAYRSLTLPAFAIAAALVLGTPAAHADNDDNTLVPNNKRLNDGV
                     VANVYTVQHQAGCTNDVKINPQLRLAAEWHTTDVLNNRNLDGDIGSDGSTPQSRAEAA
                     GYRGKVAETVAINPALAISGVDIINQWYYNPAYFAIMANCANTQMGVWSENSLDRTVV
                     VAVYGQPDQSVENRPFGPEIGLPPGVVLAENSPLDPNPDYDGSDEIEYGINWFPWILR
                     GVYPPPAYPPN"
     gene            complement(2716..2877)
                     /locus_tag="VFC01_33040"
     CDS             complement(2716..2877)
                     /locus_tag="VFC01_33040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84481.1"
                     /translation="MSQYLSAFAILLMVVSPVLIPAIIALVHRTTAGVRAVANRRRAL
                     AAAVAADAV"
BASE COUNT          676 a         1079 c         1071 g          557 t
ORIGIN      
        1 tcaacgacgg ctacaactgg aagggcgacc ccaacgccac ctggaccggc caagatatcc
       61 cgcagcagcc gcccggttca ccgccgacag ccgcgccgcc cgctgcggtc cctgcaccga
      121 tagccgtcgc cccgtatgac ccggcaaccg gcacgtacgt cggcccggac ggaaaggtct
      181 acacgcaatc cgacctcgcc aacactgcac cggaggagaa gacatggcag acaatgctga
      241 tcccgccggg gagctgacac tgctcctcga cgacgacgac gccgtcgaca tcgaggacga
      301 cggcgcgggg tgctccgcag acgacgcaga cgccgcgccg ggcccgtcgg cccacgaacc
      361 catatcagcc gcgcggcggg ccatgaagct ggcgttggcg gtcgccgttg cattggcggt
      421 tctggtggga tggttgggat ttcgcgccca ccagtcggag caagtgcacg cacagcggag
      481 tcaatttctc caggcggcca ggcagtgcgc gctgaacctg accaccatcg actggcagaa
      541 ggccgaaagc gatgtgcacc gaatcctgga cggtgccacg ggacagtttc acgacgactt
      601 cgccaagcgg gcagagccat tcattcaggt ggtcaagcaa gccaaatcga caaccgttgg
      661 tacggtcacc gacgccggac tggaatcgca aaccgctgac agcgcccagg tgctggtggc
      721 tgttaccgtg acgacctcga ataccggtgc accacagccg gatccacact cgtggcggat
      781 gcgcatctcg gtgcaggaag tcggcgacca ggccaaagtt tccaacgtgg agttcgtcgc
      841 atgagattgc taacgtcccg ccgagccgag gacccgacag acaccgactt ggtggacgac
      901 gcagccgccg agctggaatt cgtaaccggg caggacgaga ccgaggtcac cgaacgggcc
      961 acggacgccg acggatccga ccacgacgac cagacgcgga ttgcaaggcg tcgcattcga
     1021 tggtcgaaag cgatcgccta cggattaata ccgggcttgg cgcttctgct ggcgtctgcc
     1081 gcgggttatc tgaaatggca ggatggcacc acccgcgagg ctcaggcagc cggcgcggaa
     1141 tccgtgcggt ccgccatcga cagcacagtt gcgctgctgt cgtaccggcc cgacaccgtg
     1201 gagaaggacc ttgacgctgc gaagggcaga ctgaccggaa cattcctgaa cgcctacaca
     1261 tcgctgactc gtgacgtggt gattccaggg gccaagcaga agcaggtatc cgcggtggcc
     1321 actgtcccgg cggcggcgtc ctcgtctgcg accccaacgc acgccgtggt gctgctgttt
     1381 gtcgaccaga ccgttaccgt cggccaaagc gcgccgacca acaccgcatc cagcgttcgg
     1441 gtcaccctcg ataaggtcga cggccgttgg ctgatctcgc aattcgaccc ggtgtgatcc
     1501 aaatggtgaa acggcaatcc gcaacagccc gaaccccggg taagtcggtg ggagcgcttt
     1561 tcgccactgc ggccacgttg agtgccgcga cgctggggct ggctccgaac gctcgcgccg
     1621 acgtcgtggc ctacctcgtc aacgtgcatg tacgacctgg ctacaacttc cccaacgctg
     1681 atgcggccct tggctatggc tacggcatct gcgacaaggt ggctgctggc cgcggcttcc
     1741 ctgacgtcat ggccgacgtg agggccgact tcaacacgtc cgacgactac caggcctcgt
     1801 atctgatctc gcaggcggtc aacgaacttt gccccgcgca gatctggcag gtgcggaact
     1861 cggcagcgca ttaccagtcg ccccccggcg tgaccccctg acagaaagga gacgaacatg
     1921 gcgtaccggt cactgacact gcctgccttt gcgattgctg ccgccctggt gctgggtacg
     1981 cccgccgcac atgccgacaa cgacgacaac acgctcgtac ccaacaacaa gcggctcaac
     2041 gacggcgtgg tcgccaacgt ttacaccgtt cagcatcagg cgggctgcac caacgacgtc
     2101 aagatcaatc cgcaactgcg actggcggct gagtggcaca cgacggacgt gctgaacaac
     2161 cgcaacctcg acggcgatat cgggtcggac gggtcaacgc cgcaaagcag ggccgaggcg
     2221 gccggctacc ggggcaaagt ggcggaaacg gtggcgatca atccggcgtt ggcgatcagc
     2281 ggagttgaca tcatcaacca gtggtattac aacccagcct atttcgccat catggccaac
     2341 tgcgccaaca cccagatggg ggtgtggtcg gaaaacagcc tggatcgcac cgttgtcgtg
     2401 gccgtatacg gacagcccga tcagtccgtc gagaacagac cgtttggacc ggaaattggg
     2461 cttccacctg gggtggtcct cgcggaaaac tctccgctcg atcccaatcc ggactacgac
     2521 ggcagcgacg agatcgagta cggcatcaac tggttcccgt ggatcctgcg cggtgtctac
     2581 ccgccgccgg cgtacccgcc gaattagggg tccgggtcgc caacgaggga cgtgtgtatt
     2641 gcacttgttg cgcaacatct ttcgctgcgt ccctcgcaaa gcgctgcgat cagatcgcca
     2701 aagacattcg ccgatctaca ccgcatcggc tgcaactgcg gccgcgagcg cccggcgccg
     2761 gttggcgaca gcgcgaactc cggcggtggt gcggtgaacc aaggcgatga tcgccgggat
     2821 gagcaccggg gacacgacca tgagaaggat ggcgaaagcg cttaggtact gcgacatggg
     2881 tgcactcaca tttctgtatg aatcgggtgg gctgagactt gaatccgaag gggcgctctc
     2941 tccgcgtccc ttccgtgatc cggttgtgtc ttaagtgtct cgaggtccgg ccgtgtgtgc
     3001 gtctcccgat cggacgattt tcctctcccg aaaggggcaa agcgagccgc agcgccggtg
     3061 tcgaattgac attgccggcg cgattggccg gagtacggtg agatagctgc agcgaaggca
     3121 acggcgatgg gaacgctgat accgggtggc atgaatagtt tgctctggcg gtgggattcg
     3181 atccgctccg ccagcggtcc tcgtgtgcgg gtggactagc gatgagcaat cccgacgaca
     3241 cacaacgcgg tctcgctacg ggaatcgtcg ccgagctcga ggtggagggc taccacgacg
     3301 ctgagcccgt ggggcgtggc gggttcggtg tcgtctatcg ctgtcggcaa ccgtcactcg
     3361 accgtgtcgt cgcgatcaaa atc
//
LOCUS       DASRJA010000731        10726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2643210,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000731 DASRJA010000000
VERSION     DASRJA010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10726
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2643210"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2534)
                     /locus_tag="VFC01_33045"
     CDS             complement(<1..2534)
                     /locus_tag="VFC01_33045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN84482.1"
                     /translation="MGPSTGLFDVGGQRLAAPPVRGRAGELKVIGALVAALARGRGGV
                     LVIEGPPGIGKSRLLTEVMALADKGGVRTLFGEAFEYQQTVPFFVLFMATLRADPPVG
                     DADALRRLGGSADLRYWVVHDLADAIDAAAAQTPLAIVLEDIHWADNSTLLALRSLAS
                     PRPDVAVLWVLTARSGAGGPGVQETLSVLQRAGAAVVRVAAMSASAVADMVCDAVRAN
                     ADESLLNLAAKAHGNPFLVSELVGGLDEEGRLTVSGGRAVASGHGLPRRLGAGMQQRL
                     DVLSSDAGEVVRVASVLPDRFSAGLLAAMLQRQPASLMSALAEAVRADLLVEDGEQLR
                     FRHDLLREATRQSLSQSLRRAMERQSASIMLGMGAAPAEVAAQLVRSAEPGDREAIDA
                     LRQAAQSVGRSDAGAAADLSKRALELLPADDAEHGPLVAETVGLLNRASRYQEAEGLA
                     VAALSEAASPQEEAEIRLRRATLIKHSAQWRVEENRRALQLGDISEVTRARHLGWLAY
                     NLTFHDQRGQRRAAATEAAAAAASTGDLEATILADVTLAVLDGGEGYAGRAVGRLEEL
                     CALARASGVTAAHDLAALHYPLLLAAVGRLDDAAARIADGTQQARREGNAMALDMWTL
                     FDGIVHLTAGRLSSARAATESLPPPQPTAVTELNMMRMVILADVAARTDDRTLLLQMV
                     NDARDAYSTASSTVGRGTAHVLALAAWQRDDVHDAMRWLGGDITLFGTPLWPLVLDRV
                     ILGARVASAGGDAGLRARVLQAADVLERERPAIPLFSGVAGHARGILERDVQAVVAAA
                     DVLHSSSRPLLYAAAAEDAGDELARTDRGDEALDQLNAAFDTYLRH"
     gene            complement(2927..3691)
                     /locus_tag="VFC01_33050"
     CDS             complement(2927..3691)
                     /locus_tag="VFC01_33050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN84483.1"
                     /translation="MGLLDEKVVLITGTGGGLGRVAAQTFAREGAKVVGCDIKVDANN
                     ETVELIGRAGGEMTGIAPIDLTDPDQAQKMIEDAVAAYGGIDVVCNNAGIQYFGPMPD
                     FSIDDWRATITGELDIPFFVSKFAWPHLVQRGGGVIINIASHAGMIAAETPPWVGHAA
                     AKAGVIAMTRQLALEGARHGIRAVAISPGPFLTPASDRDLGNNQAARDAITSKTLLKR
                     FGQPEEIAELAAFLASDRASYITGANYPVDGGATAW"
     gene            complement(3861..4778)
                     /locus_tag="VFC01_33055"
     CDS             complement(3861..4778)
                     /locus_tag="VFC01_33055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZN84484.1"
                     /translation="MARTVQFSEYGGTDVLAAVDAPALTAGPGQVRIEVRAAGINPID
                     WKIMRGFMREIIPLTLPAGLGSDVAGVVDQVGTGVTEFQVGDEVMGRSLTPAISESAL
                     ADPANLITKPADIGWDVAGSVAGAGGTAWAVLDQLGIKAGETLLVHAAAGGVGTFAVQ
                     LAVARGARVIGTASERNFEYLRSIGAEPVCYGDGLLERVRAVAPDGVDAVLDASGRGE
                     IPLSIELAGGPDRVLTLVAFDAADTGIQVFVGATEDDWDPALREIVSLLEQRRLTVPI
                     WRTFPLAETAAALDASSAGHLNGKIVVLP"
     gene            4913..5377
                     /locus_tag="VFC01_33060"
     CDS             4913..5377
                     /locus_tag="VFC01_33060"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZN84485.1"
                     /translation="MAQERWLNENQQRFWRLYVDVQHHLEGVLHRELLTGSGLSSAEF
                     AVLVPLSETPAEKLRARDLCRELGWDRTRLSHLVARMQQRGLVRRSGCADDARGSVVE
                     LTHEGRRAITDAAPAHTDAVRRYLIDLLDDDEIASMTDVFQRVLEGLDPQRD"
     gene            complement(5952..9041)
                     /locus_tag="VFC01_33065"
     CDS             complement(5952..9041)
                     /locus_tag="VFC01_33065"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZN84486.1"
                     /translation="MAVVDDLGHSGMDVPPPSEDFGRQPPQDAAAEQAVLGGMLLSKD
                     AIADVLERLRPGDFYRPAHQNVYDAILDLYGRGEPADAVTVAAELDRRGLLRRIGGAP
                     YLHTLISTVPTAANAGFYAGIVAEKALLRRLVEAGTRVVQYGYAGAEGADVTEIVDRA
                     QAEIYDVTEGRTSEDFVALEDLLQPTMDEIDAIASNGGIARGVPTGFVELDEVTNGLH
                     AGQMIIIAARPGVGKALALNTPLPTPTGWTTMGDVAVGDWLLGADGEPTQVVAATDIM
                     FGRPCYEVEFSDGTVIVADALHQWPTGYGVRTTAELRWGMDTIAAAGSMARYDGRGAT
                     TLMAPVLQIEAVHRVRTVPVRCVQVDNGAHLYLAGPGMVPTHNSTLGLDFLRSCSIKN
                     QMASVIFSLEMSKSEIVMRLLSAEAKIKLSDMRSGRMSDDDWTRLARRMSEISEAPLY
                     IDDSPNLTMMEIRAKARRLSQKAGLKLIVVDYMQLMTSGKKYESRQQEVSDFSRSLKL
                     MAKELDVPVVAISQLNRGPEQRTDKKPMISDLRESGCLTANTRILRADNGAEVTFGEL
                     MRTGERPLVWSLDERKRLVARPMTNVFPSGHKEVFKLRLASGREVEATANHPFMTFDG
                     WTPLGELKVGDRLAVPRRVPEPVHTERMDESEIILLAHMIGDGSCVKRQPIRYASIDE
                     ENLAAVATAAKHFGVTAIRDDYPAARATTMRLPAPYRLTHGKRNPIAAWLDGLGLFGK
                     RSYEKFVPQKVFALSNDQIALFLRHLWATDGSVRWDAKVQQGRVYYASSSRQLIDDVM
                     QLLLRLGINSRIYRARKAGYRDSWHLNIFGCENQRKFLKFVDVNGEKFFAAREVLSHL
                     KQVEPKANSDTIPAEVWRRVRDALTESDMSHRDFAELMGTKFCGSTLWKHAVGRKRLH
                     KAAALLDDSELHELTTDDVFWDRIIGITSIGEHDVYDGTVSGTHNFVGNLVSLHNSLE
                     QDADMVILLHRPDAFERDDPRGGEADLILGKHRNGPTKTITVAHQLHLSRFTNMAKA"
     gene            complement(9526..9981)
                     /gene="rplI"
                     /locus_tag="VFC01_33070"
     CDS             complement(9526..9981)
                     /gene="rplI"
                     /locus_tag="VFC01_33070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZN84487.1"
                     /translation="MKLILTAEVDHLGTSGDIVEVKDGYGRNFLLPRGLAIVASRGAE
                     RQAEEIRRAREAKAVQGLEHAKELKSAIEGLGSVELSVKAAGDSGKLFGSVTAADVVA
                     AIKKAGGPNLDKRTVQLSKAHIKFVGTHPVVIRLHPDVDASVSLSVVAS"
     gene            complement(9998..10252)
                     /gene="rpsR"
                     /locus_tag="VFC01_33075"
     CDS             complement(9998..10252)
                     /gene="rpsR"
                     /locus_tag="VFC01_33075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZN84488.1"
                     /translation="MAKATKRRPAPEKPIKTRKCVFCSKKGQTIDYKDTQLLRTYISE
                     RGKIRARRVTGNCVQHQRDIAIAVKNAREVALLPFSASTR"
     gene            complement(10291..>10726)
                     /locus_tag="VFC01_33080"
     CDS             complement(10291..>10726)
                     /locus_tag="VFC01_33080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZN84489.1"
                     /translation="FTVAATPRTFDRNSNEWKDGEALFMRCNIWREAAENVAESLTRG
                     ARVIVTGRLKQRSFETREGEKRTVVEVEVDEIGPSLRYATAKVNKASRSGGGGGGFGS
                     GGGGGSRGGGSGGGSEQPKQDDPWGSAPASGSFSGSDDEPPF"
BASE COUNT         1871 a         3732 c         3214 g         1909 t
ORIGIN      
        1 tcgtggcgca ggtaggtgtc gaatgcggca ttgagctgat ccagcgcctc atcaccccgg
       61 tcggtgcgtg cgagttcatc gccggcgtcc tcggcagctg cggcgtacag aagcggccgc
      121 gacgacgagt gcagcacgtc ggcggcagcc accactgcct ggacatcacg ttcgagtatc
      181 ccgcgggcat gcccggccac ccccgagaac agcgggatcg ccggccgctc acgctcgagc
      241 acgtcggcgg cctgcaggac acgtgcgcgc aagccggcat caccgccggc cgacgccacc
      301 cgcgcaccca agatcacccg atccaagacc aggggccaga gtggtgtccc gaacagcgtg
      361 atgtcgccgc ccagccagcg catcgcgtca tggacgtcgt cgcgctgcca cgccgccagc
      421 gccagcacgt gcgccgttcc ccgacctacc gtagatgagg cagtggaata ggcgtcgcgc
      481 gcgtcattga ccatctgcag caacaacgtt cggtcatcgg tgcgcgccgc cacgtcggcc
      541 agaatcacca tgcgcatcat gttcagctcg gtcacggctg tcggctgtgg gggcggcagg
      601 gattcggtcg cggcgcgggc gctggacaac cggcccgcag ttaggtggac aatcccgtca
      661 aacagcgtcc acatatcaag ggccatcgcg ttgccttccc ggcgggcttg ctgtgttccg
      721 tcggcgatcc gggccgcagc gtcgtccaac cggccgaccg ccgccagcag gagcgggtag
      781 tggagtgcgg ccaagtcatg agctgcggtc acaccactcg cgcgggcgag cgcgcaaagc
      841 tcctcgaggc ggcctacggc gcggcccgcg tacccctccc caccgtcgag cacagcgagg
      901 gtgacatcgg ccaggatcgt ggcctcgaga tcaccggtgg atgccgccgc cgcggcagcc
      961 tcggtggccg ccgcgcgccg ttgcccccgt tggtcgtgga acgtcaggtt gtaggccagc
     1021 caccccagat gccgggcccg ggtgacctcg ctgatgtcgc ccaactgcag cgctcgacga
     1081 ttctcctcga cgcgccactg tgcactgtgc ttgatgagcg tcgcgcggcg cagccgaatc
     1141 tcggcctcct cttggggcga cgccgcctcc gagagcgccg caacggccaa cccttcggcc
     1201 tcctgatagc ggctggcccg gttgagcaac cccaccgtct cggcgaccag tggcccgtgc
     1261 tcagcgtcgt cggcgggcaa caactccaac gctcgcttgc tcaggtccgc ggccgcaccg
     1321 gcatcgctgc ggccaaccga ttgggcggct tggcgcagcg cgtcgatcgc ctcccggtcc
     1381 ccgggctcgg cgctgcgcac cagctgcgcg gccacctcgg ccggcgccgc gcccataccg
     1441 agcatgatcg acgccgattg gcgctccatg gcgcggcgca gcgactgcga caatgactgc
     1501 cgggtggcct cgcgcagcaa gtcgtgccga aaccgcaact gctcaccgtc ttcgacgagc
     1561 agatccgcgc gcaccgcctc ggcgagcgcc gacatcaacg acgcgggctg gcgttgcagc
     1621 atcgcggcca gcaggccggc cgagaaccgg tccggcagca ccgaggccac ccgcaccacc
     1681 tcgcccgcat cgctggagag cacgtcgagt cgctgctgca taccggcgcc cagccggcgc
     1741 ggcagtccgt gtccgctggc caccgcacgc ccgccgctga cagtgagccg gccctcctcg
     1801 tccagacccc ccaccagctc gctgaccagg aagggatttc cgtgcgcctt ggcggccaga
     1861 ttcaacagcg actcatcggc gttcgcgcgc accgcgtcgc agaccatgtc ggcgaccgcg
     1921 ctcgccgaca tcgcggccac ccgcacaacg gcggcacccg cgcgttgcag caccgacaag
     1981 gtctcctgca cccccggccc gccggccccg ctgcgggcgg tcagcaccca cagcaccgcc
     2041 acatccggcc gcggactggc cagcgaccgc agcgccaaca acgtgctgtt atcagcccag
     2101 tggatgtcct ccagcacaat cgccagcggg gtctgcgccg ccgccgcatc aatcgcgtcg
     2161 gccagatcgt gcaccaccca atagcgcaga tccgccgagc cacccagccg gcgcaacgcg
     2221 tcggcgtcgc ccaccggcgg atcggcccgc agcgtggcca tgaacagcac gaaaaacggc
     2281 accgtctgct gatactcgaa ggcctcaccg aacaaggtgc gcaccccgcc cttgtcggcc
     2341 agcgccatca cctcggtcag caaccgactc ttcccgatac ccggcgggcc ctcgatgacc
     2401 agcacgcccc cgcgcccccg cgccagcgcc gccaccaacg cgccgatcac cttcaactcg
     2461 cccgcccggc cccgaaccgg cggggctgcg agccgctggc cgccgacatc aaaaaggccg
     2521 gttgaaggcc ccacgaggtg tcccgtctac cgttcgccga actgccactg cagtgcagtc
     2581 attgtaggca tctgcccacg tcatcgaccc gcattgctgt cgatcgctcg gcatgcgcgc
     2641 gatctaggca gcccgtcggc gcgggcagcc aaaaaccagc ggcgacgacg acgaacggcg
     2701 ggtgatcgcg cgggcaagca gaaccgccag ttcatcggct cgccgccacg tcacggtgat
     2761 gcgccggctg agtcgcatca gtgcacgcag cgagcagtca gcattcgcca ggatcgcgat
     2821 tcccgcgcca tcgctgccga gttcgcgtca cttcgacaag atctggtcac cgacgatccc
     2881 aaggctcgga cgcgccatca cctcgcattg gccgtgcctg tggctcctac caagcggtcg
     2941 cgccgccgtc gaccgggtag ttggcgcctg tgatgtacga cgcccggtca gaagcgagga
     3001 aggccgcgag ctccgcgatc tcctcgggtt gaccaaaccg cttgagaagc gtcttgctgg
     3061 ttatcgcgtc ccgggccgcc tggttgttgc cgaggtcgcg gtcgctggcc ggggtcagga
     3121 aaggcccggg gctgatcgcc accgcgcgga tcccatgccg cgcgccttca agcgctaatt
     3181 gtcgcgtcat ggcgatgacg ccggctttcg ctgcggcgtg cccgacccac gggggagtct
     3241 ctgcggcgat catgcctgcg tgggacgcga tgttgatgat gacaccgcca ccgcgctgga
     3301 ccaggtgcgg ccacgcgaac ttcgacacga agaaggggat gtcgagttcg ccagttatgg
     3361 tcgcccgcca gtcatcgatg gagaaatcgg gcatcggacc gaagtattgg atgcccgcgt
     3421 tgttgcagac cacgtcgatc ccgccgtagg cggccacggc atcctcaatc atcttctgcg
     3481 cctggtcggg gtcggtgagg tcgatcggcg cgatgccagt catctcaccg ccggcgcgac
     3541 cgatgagttc gaccgtctcg ttgttggcat caaccttgat gtcgcacccg acaaccttgg
     3601 cgccttctcg cgcaaaggtc tgtgccgcga cgcggcccag acctcctccg gtgccggtga
     3661 tcagcacgac cttctcgtcg agtaatccca tagcttcctc ctgatctgaa ctgcgcgttg
     3721 atggattcgt ggctggcgct gcaacggtgg tggcaatcgg cctgcacgcc agcgctacac
     3781 cggcaatgcg gctcgtcagc gtcggccctt gcgcttgccc gttacccgtg gtttggcatc
     3841 acgggtaccc gacgtcgggg tcacggcagg acgacgatct ttccgttgag gtgcccggca
     3901 ctgctggcat cgagtgctgc tgccgtctct gccagaggaa aggtccgcca gatggggacg
     3961 gtaagccgcc gctgctcgag aagggagacg atctcgcgca gcgcagggtc ccaatcatcc
     4021 tctgttgcgc cgacgaagac ttgaattccg gtgtcggcgg cgtcgaacgc gacgagtgtc
     4081 agaacacggt cggggccacc ggcgagttca atcgagagcg gaatctcgcc acggccggac
     4141 gcatcaagga cggcatcgac cccgtctggc gcaacggcgc ggacacgttc gaggaggccg
     4201 tcgccgtagc agacgggctc ggcgccaatc gatcgcaggt actcgaaatt gcgctcactc
     4261 gccgtaccaa ttacccgtgc cccgcgcgcg accgcgagct gaaccgcgaa cgtacccacc
     4321 ccaccggcgg cggcatgcac gagcaacgtc tcgcccgcct taatgcccag ttggtcaagc
     4381 accgcccacg cggtgccgcc cgcgccggcc acgctgccgg ccacgtccca gccgatgtcc
     4441 gcgggtttgg tgatgaggtt ggccggatct gcaagggcgg actcggagat ggcgggcgtc
     4501 agcgagcggc ccatcacctc atcgcccacc tggaattcgg tgacccccgt acccacctga
     4561 tcgaccacac cggcgacatc ggaccccagg cccgcgggca gcgtcaacgg gatgatctcg
     4621 cgcatgaagc cccgcatgat cttccagtcg atgggattga taccggcggc ccgcacctcg
     4681 atccggacct gcccgggccc ggcggtcagc gcgggcgcat cgaccgcagc gagcacatcc
     4741 gtgccgccgt attccgagaa ctgcactgtt cgcgccatct ctacatttcc aatctgattc
     4801 gaatcgaata gttgttgcca cgttccaagg gtcgaacccc caggatgtag acgcgtcaac
     4861 aaatctagca ccttgatgtt gatgcgtcaa caaaattcgg tatgctgtca ccatggcgca
     4921 ggagcgatgg ctcaatgaga atcaacagcg cttctggcgg ctgtacgtgg atgtccagca
     4981 tcatctggag ggggtactgc accgcgagtt acttacgggc tccgggcttt ccagcgcgga
     5041 gtttgccgtg ctcgtacccc tgtcggagac accagcggag aaacttcggg ccagagattt
     5101 gtgccgagaa ctcggatggg atcggacacg gttgtcacac ttggttgcca gaatgcagca
     5161 gcggggactc gtccgccgaa gcgggtgcgc cgacgatgcc cgcggatccg tggtcgagct
     5221 cacccacgag gggcgccgag cgattactga cgctgccccc gcgcataccg acgccgttcg
     5281 ccgatacctt atcgacctgc tcgacgacga cgagatcgcc agcatgactg atgtcttcca
     5341 gcgagtactg gaagggcttg acccacaacg agattagggc gcgggtggtt cagtacatgg
     5401 agaccctgct cacgcaggct cgcaccctcg cgcgcaaggc tcactagggt attcgctaag
     5461 ccacttcaga tagtcttgca gcgcttcgtc gaatgtgtat tggggacgcc acccggtatc
     5521 caacatcgcg gtctccggcc gtccgcccgc cgactacggc ggcgatccca cccgcgaatc
     5581 gctgcgcttc gacgtgcccc acgagccacc catcgaggcc gcgacctccg atgcaacggg
     5641 caaccgccac catcagagtc tgagcgtccg gttgcctgga caagcacgca cacagcaact
     5701 ctgtcggcgt gatgccattc gctgcggcga gccggcgaca caacgagtcg aacgactcac
     5761 ccgcaccggc ccgatcctgg ccggaagcca gtcatcggag ctgtcggtgc ggccaaaaat
     5821 tattcaacta cgaactctag ttaaaacaac ggggtgccgc ttgctaatct tcgctgcatc
     5881 tcgacaggca ccttttactt cttttattga ggttaattat catcatcatc agctagcatt
     5941 aattaaggag actatgcctt ggccatgttg gtgaatcgcg acaaatgcag ttggtgcgca
     6001 acggtgatcg tcttcgtcgg accgttgcgg tgcttgccca agatcaggtc ggcttcgcca
     6061 ccgcgcggat cgtcacgctc gaacgcatcc ggacggtgca acaggataac catgtccgca
     6121 tcctgttcca gcgagttatg taaactaact aagtttccga cgaaattgtg tgtgccgctt
     6181 acggttccat cgtagacgtc gtgctccccg atgctcgtga tcccgatgat tctgtcccag
     6241 aagacgtcat ctgtggtgag ctcgtggagc tctgaatcgt ccagtagtgc ggctgcctta
     6301 tgcaagcgtt tgcgacccac tgcatgcttc cacagcgttg aaccacagaa cttggtcccc
     6361 atcaactcgg cgaaatccct atgcgacata tcactttcgg tcaacgcatc tcgaactcgg
     6421 cgccacacct ccgccggaat ggtgtcagag tttgcctttg gctctacctg cttcaggtgc
     6481 gacaacactt cccgagccgc aaagaacttc tccccgttga cgtctacgaa cttgaggaac
     6541 ttgcgctgat tttcgcagcc gaagatatta aggtgccagg agtcgcggta tccggccttg
     6601 cgggcacgat agatccgact gttgattccc aaccgcagca gcaactgcat gacgtcgtcg
     6661 atcaactgac gactgctcga ggcatagtag acgcgccctt gctggacctt ggcgtcccag
     6721 cgcactgagc cgtcggtcgc ccacaagtgg cgcaggaaca atgcgatctg atcgttcgac
     6781 aacgcgaaaa ctttttgtgg cacaaacttt tcgtagcttc tcttgccgaa gagacctagt
     6841 ccatcaagcc atgccgcgat cgggttgcgc ttcccatgtg tcaggcgata tggcgctggc
     6901 aaccgcatcg ttgtcgctcg cgcggcgggg taatcgtcgc ggatcgcggt caccccgaag
     6961 tgcttggccg ccgtagccac cgctgccaag ttttcttcgt cgatgctcgc gtacctgatc
     7021 ggctgccgct tcacacatga gccgtcgcca atcatgtggg ctagcaatat gatctcggac
     7081 tcgtccatcc gctccgtatg aacgggctcc ggcacccgcc gcggcaccgc cagacgatcg
     7141 ccaaccttca actcgcccaa cggagtccag ccatcaaacg tcatgaacgg atggttggcg
     7201 gtggcctcca cctctcggcc cgaggccagt cgaagtttga agacttcctt gtgtccgctc
     7261 gggaacacat tcgtcatcgg ccgagcgacc agccgcttgc gctcgtccag cgaccacacc
     7321 aacggccgct cacccgtgcg catcagctcg ccaaacgtca cctcagcacc gttgtccgcc
     7381 cgcaagattc gcgtattcgc cgtcaagcag cccgactcac gaaggtccga gatcatcggc
     7441 ttcttgtccg tgcgctgttc cggcccccgg ttcagctggc tgatcgcgac cacgggtacg
     7501 tcgagttcct tggccatcag cttgaggctt cgcgagaagt ccgacacctc ttgctggcgc
     7561 gactcgtact tcttgcccga ggtcatcagc tgcatgtaat cgacgacgat cagcttcagc
     7621 ccagccttct gagaaagccg gcgcgccttc gcgcggatct ccatcatcgt caggttcggc
     7681 gagtcgtcga tgtacagcgg cgcttcgctg atttcgctca tccgccgcgc caatcgcgtc
     7741 cagtcgtcgt cgctcatgcg acccgatcgc atgtcggaca gcttgatctt cgcctcggct
     7801 gacagcagcc gcatgacgat ctccgacttg ctcatctcca ggctgaaaat gacgcttgcc
     7861 atctggttct tgatcgagca cgaccgcagg aagtcgagac ccaacgtcga gttgtgcgtc
     7921 ggcaccatcc ccggaccggc cagatagagg tgcgccccgt tgtccacctg cacgcagcgc
     7981 accggaaccg tgcgcacccg atgtaccgcc tcgatctgca gcaccggtgc catcaacgtc
     8041 gtggcacccc gcccgtcgta ccgcgccatc gaccctgccg ccgcgattgt gtccatgccc
     8101 caacgcagtt ctgcggtagt ccggaccccg tagcctgtcg gccattgatg cagcgcgtcc
     8161 gcgacgatca ctgtgccatc ggagaactcg acctcatagc acggccgtcc gaacataatg
     8221 tccgtcgcag ccaccacctg cgtcggctct ccatcggcgc ccaacagcca atccccgacc
     8281 gcaacatcac ccatggtggt ccaacccgtc ggcgtcggaa ggggcgtatt gagtgccaac
     8341 gccttcccaa caccaggtcg cgccgcgatg atgatcatct gacccgcgtg cagaccattg
     8401 gtcacctcgt cgagttcaac aaagcctgtc ggaacgccgc gcgcgatacc tccgttcgac
     8461 gcgatggcgt cgatctcgtc catcgtcggc tgcagcaggt cttccagggc cacaaagtct
     8521 tccgacgtgc gtccctcggt gacgtcgtag atctcggcct gcgcgcgatc gacgatctcg
     8581 gtgacgtccg cgccctctgc gcccgcgtag ccgtactgca ccacccgggt gcccgcctcg
     8641 accaaccggc gcagcagcgc cttctccgcg acgatgcccg cgtaaaagcc cgcgttcgcc
     8701 gcggtcggca ccgtggagat cagcgtgtgc agatacggcg ccccgccaat ccggcggagc
     8761 aaccctcgac gatccaactc ggcggccact gtcaccgcat ccgcgggctc gccgcggccg
     8821 tacagatcga ggatcgcgtc gtagacgttc tgatgcgcgg gccggtagaa gtcaccggga
     8881 cgcagccgct ccaatacgtc cgcgatggcg tccttgctca gcagcatgcc tccgaggacc
     8941 gcctgttcgg ccgccgcgtc ctggggtggc tgacgaccga agtcctcact gggtggcgga
     9001 acgtccatac ccgaatggcc gaggtcgtcc acgacagcca ttgcgcgttc cacctcccct
     9061 cacctgatcg aacgtatgtt cgtctacgct ctttcgagcg taagtcagac gaccgacatc
     9121 tgccacctcg cgaaccgcaa gactgccttg cgacggccaa cgctagacgt tgctgggagc
     9181 cctacaaaac ggccttgtta atggacttgt gtatggggtg tggatatccg tgggcagcca
     9241 tgttgaggcg ttggggagaa cctgtggact aacgactttc tttggtgcat ccccgcagat
     9301 aagcgcacca caaactacgt gcgtatctgt ggatgacaat tccctcggcg tgtcggccga
     9361 ggttgcgatc tcgggcgtgt tgtgtttcgg gtaagggccg aacaggttaa cagctcgtta
     9421 gcttcgctgc gacccgaatg tggggcaaag ttcgccagag ggtacagcaa cgaccgggtg
     9481 gagatcgatc atggccttca cccggtcgaa ttgacgctgc ggtaactaac tcgcgacgac
     9541 gctcagggac accgaagcgt ccacatccgg gtgcagccgg atgacgaccg ggtgcgtacc
     9601 gacgaacttg atgtgcgcct tggacaactg aaccgtccgc ttgtccagat tcggtccgcc
     9661 ggccttcttg atcgccgcaa cgacgtccgc cgccgtcacc gaaccgaaca gcttgccgct
     9721 gtcgcccgcc gccttcaccg acagctcgac cgagcccagg ccctcgatcg cgctcttgag
     9781 ctccttggcg tgctcgaggc cctgcaccgc cttggcctcg cgggcccgac ggatctcctc
     9841 ggcctgccgc tccgcgcccc gtgacgcgac gatcgccagt ccacgcggca gcaggaagtt
     9901 gcggccgtag ccgtccttga cctccacgat gtcgccagac gtcccgaggt ggtcgacctc
     9961 tgcagtgaga atcagtttca ttttttttcg tccctcccta ccgcgtcgac gcgctgaacg
    10021 gcaggagcgc cacctcgcgg gcgttcttga ccgcgatggc gatgtcgcgc tggtgctgca
    10081 cgcagtttcc cgtcacacgg cgggcacgga tcttgccgcg ctcgctgatg taggtgcgca
    10141 acagctgggt gtccttgtaa tcgatcgtct gccccttctt ggagcagaac acgcacttgc
    10201 gcgtcttgat cggcttctcg ggcgccgggc gccgtttggt ggccttggcc atgtgtgtat
    10261 ctctttctgg aattgctgat tagttgtaag tcagaagggc ggttcgtcgt cgctgccgga
    10321 gaacgaaccg gacgcgggcg cactgcccca cgggtcgtcc tgcttgggct gttcggagcc
    10381 gcccccggag cctcccccac gggaacctcc gccgccaccg ctgccgaatc cgccgccacc
    10441 gccgccgctg cggctcgcct tgttcacctt ggcggtcgcg tagcgcaacg acgggccgat
    10501 ctcgtcgacc tcgacctcga ccacggtgcg cttctcgccc tcacgggtct cgaaggaacg
    10561 ctgcttgagc cggccggtga cgatcacccg ggcgccgcgc gtcaggcttt cggcgacatt
    10621 ctccgcggcc tcgcgccaga tgttgcagcg catgaacagc gcctcgccgt ctttccattc
    10681 attggaattc cggtcgaagg tccgcggggt tgcggccacg gtgaag
//
LOCUS       DASRJA010000732         4910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2072667,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000732 DASRJA010000000
VERSION     DASRJA010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2072667"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..89)
                     /locus_tag="VFC01_33085"
     CDS             complement(<1..89)
                     /locus_tag="VFC01_33085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HZN84490.1"
                     /translation="MRVLVQRVTSASVTVDGDVVGAISPDGQGL"
     gene            153..557
                     /locus_tag="VFC01_33090"
     CDS             153..557
                     /locus_tag="VFC01_33090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84491.1"
                     /translation="MTPGTWMRRLPPAAVTDVPVLVVTVLAWLTVALFWAHVAAGLVL
                     VALVALHLQTRHRLPWRGAPPRHQLAYGMFIVAATAMAASGLLRLAGVPPQYVWHGGI
                     SYTVLGLKALHVWSIRRALRARVRRRKGIQHE"
     gene            550..1629
                     /locus_tag="VFC01_33095"
     CDS             550..1629
                     /locus_tag="VFC01_33095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009477554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZN84492.1"
                     /translation="MSEERFDTVLIGAGQAGLAVGYYLTRQRRNFAIVDAGERVGHSW
                     DRRWESLRLFTPAHFTYLPGMRFPGPRGHVPSKSEMADYLRSYAARFALPVRMAWRVD
                     ELSRDDDGYVINSAGRRLRADSVVVATGPAMRPQVPDVADQLDPATVSLHSTDYRNPD
                     QLRDGTVLIVGAGNSGAEIALDVAPAHGVILAGRDTGRLPVSIGGPLYRVMNLVLTAE
                     SRLGRRLMANVNSGKGTPLVRVHPRDLAEAGVERAPRVVGQVEGHPRLEDGRVLGVAN
                     VIWCTGYRPDYSWIALPHFPHQEQPAHRRGAVVGNPGLYFVGLPFLYRMASSLVAGVG
                     PDAKHIARLIARSRAADVDLSGYTP"
     gene            1646..2242
                     /locus_tag="VFC01_33100"
     CDS             1646..2242
                     /locus_tag="VFC01_33100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN84493.1"
                     /translation="MKLNSIERAFVNNPVRAAHQHRRESAWFRRLAGGDLSGQHVLEV
                     GCGQGVGAEVILDRLGAEQVTAFDLDGSMVERARRRLHARPVSLSVGDVCAIEQASGA
                     FDTVVDFGIIHHVPNWQESIAEIGRVLRPGGLLLFEEIPRHMLDTWVFRTFTVHPREN
                     RFEADEFAAELARHGLHGTGRIEPHFGGALFVGAARKT"
     gene            complement(2239..2850)
                     /locus_tag="VFC01_33105"
     CDS             complement(2239..2850)
                     /locus_tag="VFC01_33105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HZN84494.1"
                     /translation="MWKRTTLIAAGALAVLAISTGCSSSNESEHPAGHSSSAAAAPTS
                     SAAAEAHNDADVMFAQHMIPHHQQAVEMSDMLLAKQGIDPRIIELAKQIKAAQGPEIQ
                     QMQGWLTQWGTPSMPPMSGHDMPSMQGMMSDTDMTALKDAQGVDAGKLFLTQMIAHHE
                     GAIAMAQTEIKDGQYPAAVAMAHAIVTTQQHEIDTMKGILTTL"
     gene            2909..3115
                     /locus_tag="VFC01_33110"
     CDS             2909..3115
                     /locus_tag="VFC01_33110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006554281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HZN84495.1"
                     /translation="MSTTSVTVMGMSCGGCASSVREEIGDIRGVSTVDVDLASGTVII
                     TSEGPVDSVAIKTAVEGAGYQLAR"
     gene            3115..4005
                     /locus_tag="VFC01_33115"
     CDS             3115..4005
                     /locus_tag="VFC01_33115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84496.1"
                     /translation="MNAAQKIAAFVAGLVMVFTVAIWVGRTVGPEGGVDVRQAPPHED
                     SHAAHDAGAPEPLGGLAVVQDGYTLDLTEDRGRAAKDVPLRFRILDGNGAPVTRYVEK
                     HEKLLHLIVVRSDLAGFQHVHPVLESDGTWRVPVDLSRAGDYRIFADFTPSGGPALTL
                     GANLHVGGEYDPQPLPPVAATTVVDGYTVALSGAPKANEPSMLTLAVSRNGKPVTDLQ
                     PYLGAYGHLVALRAADLGYLHVHPMGEPGDGITAAGPEIAFHATFPSAGAYRLFLDFQ
                     HDGVVRTAAFTVTVEDGEAT"
     gene            4002..>4910
                     /locus_tag="VFC01_33120"
     CDS             4002..>4910
                     /locus_tag="VFC01_33120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZN84497.1"
                     /translation="MTSVELSIDGMTCASCAHRIEKKLNKIDGVTATVNFATEKARVE
                     YGTEVTPEQLVATVDEAGYQAHLPEEPVEAAAEQDPTASLLQRLLISAALTIPVIAMA
                     MVPALQFTYWQWLSLALAAPVVVWGAWPFHRAAWANLKHGTATMDTLISMGTLAALGW
                     SVYALFWGTAGMPGMKHAFELTIARTDGTGNIYLEAAAGVTTFILAGRYFEARAKRRA
                     GAALEALLHLGAKDVSVLRSGVEQRIPIDQLSVGDEFVVRPGEKIATDGVVVDGNSAV
                     DASMLTGESVPVDVGPDDQVVGATVNV"
BASE COUNT          813 a         1516 c         1675 g          906 t
ORIGIN      
        1 aggccttggc cgtccgggct gatcgcaccg acgacgtcac catcgactgt gacggatgcc
       61 gacgtgaccc gttgcacgag aactcgcatg accgcagatg ctgccagacg gcatttgact
      121 tcaagttgac ttgaagcacc acactcgggc ccgtgacccc cggaacgtgg atgcgtcggc
      181 ttccaccggc agccgtgacg gacgtgccgg tgctggtcgt gacagtgttg gcatggctga
      241 ccgttgcgct gttctgggcg catgtggcag caggactggt gctcgtcgcg ctcgtcgcgc
      301 tgcatctgca aacacgacac cgactgccct ggcgcggtgc cccgccgcgc catcagctcg
      361 cctacgggat gttcatcgtc gcggcgacgg ccatggcggc gagcggtctg ttgcgcttgg
      421 ccggcgtacc accgcaatac gtatggcacg gcgggatcag ttacacggtg cttgggctta
      481 aggcgctgca tgtctggtcg attcgacgcg ccttacgggc gcgcgttcgc cgccggaaag
      541 gaatccaaca tgagtgagga gcggttcgac accgtgctta tcggcgccgg ccaggcgggg
      601 ttggccgtcg ggtattacct gacgcgacaa cgtcgcaact tcgccatcgt cgacgctggc
      661 gagcgcgtcg gccattcatg ggaccggcga tgggagtcgc tgcgtttgtt caccccggca
      721 cacttcacat atctgccggg catgcggttc cctggcccac gcggccatgt gccgtcaaag
      781 agtgagatgg ctgactactt gcgttcatac gcggctcggt tcgcactccc agtccggatg
      841 gcctggcggg tcgatgaact cagccgcgat gacgacggtt acgtgatcaa ctcggcaggc
      901 cggcgtctgc gggcggacag cgtcgtggtc gcgacggggc cggcaatgcg accccaggtg
      961 cccgacgtgg ccgaccaatt ggatccggcc accgtgtcgt tgcattcgac ggactatcgc
     1021 aatcccgacc agttgcgcga cggaacagtg ctgattgtcg gtgccggaaa ctccggcgcg
     1081 gagatagcac ttgatgtggc gcccgcacac ggcgtcatac tcgccggccg tgacaccggc
     1141 cggctgccgg tgtccatcgg tggcccgctc taccgggtga tgaatcttgt gctaaccgcc
     1201 gagagccgcc tcggccgacg actgatggcg aatgtcaact cgggcaaggg cactccgctg
     1261 gtccgggtgc atccgcgcga tctcgcggaa gcaggcgtcg agcgtgcgcc gcgggtcgtc
     1321 ggtcaggtcg agggtcaccc ccgcctggag gatgggcgcg tgctcggtgt tgccaacgtc
     1381 atttggtgca ccggataccg gcccgattac tcgtggatcg cgctgccgca cttcccgcac
     1441 caagaacaac ctgcgcatcg gcgaggcgca gtcgtcggga atcccgggct ctacttcgtc
     1501 gggctgccgt tcctctaccg gatggcgtca tcgctggtcg ccggtgtcgg gccggatgcg
     1561 aaacacatcg caagactcat cgcacggagt cgggcggcag acgtagacct ttcgggctat
     1621 accccatagg ggtaccgtcc gcgtcatgaa gctcaactcc atcgaacgcg catttgtgaa
     1681 caaccccgtg cgtgccgcac atcagcaccg ccgggagtcg gcctggtttc ggcggctggc
     1741 gggcggcgac ctgtccggcc agcacgtgct cgaggtcgga tgcgggcagg gcgtcggcgc
     1801 cgaagtgatc ctcgatcgac tcggtgccga gcaggtgaca gcctttgatc tcgacggctc
     1861 gatggtcgag cgggcgcgtc gacgactgca cgcccgtccg gtgtcgttgt cggtcggcga
     1921 cgtgtgcgcg atcgaacagg catcgggcgc attcgacacc gtggtggatt tcgggatcat
     1981 ccaccacgtg ccgaactggc aagagtcgat cgccgagatc ggacgcgtat tgcggcccgg
     2041 aggtctgctg ctgttcgagg agatcccccg ccacatgctg gacacctggg tgttccgcac
     2101 attcaccgtc catccgcgtg aaaaccggtt cgaggcagac gaattcgccg ccgagctggc
     2161 gcgtcacgga ttgcacggaa ccggtcgaat cgagccgcat ttcggcggcg cactattcgt
     2221 cggcgcggcc cgcaagacct agagcgtcgt caaaatgccc ttcatcgtgt cgatctcgtg
     2281 ctgctgggtg gtgacgatcg cgtgagccat cgcgacggcg gctggatact gaccatcctt
     2341 gatttcagtc tgcgccatgg cgattgcgcc ttcgtgatgc gcgatcatct gcgtcaggaa
     2401 cagcttgccg gcgtcgacgc cctgcgcgtc tttcagtgcg gtcatgtccg tgtcggacat
     2461 catgccctgc atgctcggca tgtcatggcc ggacatcggc ggcatcgacg gagtacccca
     2521 ttgagtcaac cagccctgca tctgctgaat ctcgggaccc tgggcggcct tgatctgctt
     2581 ggccagctcg atgatccggg ggtcgatgcc ctgcttggcg agcagcatgt cgctcatctc
     2641 gaccgcctgc tggtggtggg gaatcatgtg ctgggcgaac atcacgtcgg cgtcgttgtg
     2701 ggcctcagca gcggcgctac tggtcggagc ggcggccgcg ctcgacgagt gtccggccgg
     2761 gtgctcggac tcgttggagc tgctacagcc ggtcgagatg gccagcactg ccaaggcgcc
     2821 ggcggcgatc agggtcgttc gcttccacat agtcatgggt ccctttctac acattgcata
     2881 ccctaggggg gtatggtaca catggttcat gagcacaacc tcggtcaccg tcatgggtat
     2941 gagctgcggc ggatgcgcct catccgtgcg tgaagagatc ggcgacatcc gtggcgtcag
     3001 cacggtcgac gtggatctag cgagcggcac ggtgatcatc accagcgaag gcccggtcga
     3061 tagcgtcgcg atcaagaccg ccgtcgaggg agccggctac caactggcac gctgatgaac
     3121 gccgcacaga agatcgccgc tttcgtcgcc ggcctggtga tggtgttcac cgtcgcgatc
     3181 tgggtcggta ggaccgtcgg acccgagggc ggggtggatg tgcgacaggc gccgccacac
     3241 gaagacagcc acgccgcgca cgacgccggc gcccccgagc cgctcggtgg gctggcggtg
     3301 gtccaggatg gctacacgct tgacctgacg gaagaccgtg gccgggccgc aaaggacgtg
     3361 ccgctgcggt ttcggattct cgatggcaat ggcgcgccgg tgacgcggta cgtcgaaaag
     3421 cacgagaagc tgttgcatct gatcgtggtt cgcagtgacc tggccggctt tcaacatgtg
     3481 catccggtgc tggaatcgga tggaacgtgg cgcgtgccag tcgatctcag ccgagcaggc
     3541 gactaccgca tcttcgccga cttcacgcct tcgggtggcc cggcgctgac actgggagcc
     3601 aatcttcacg tcggcggcga gtacgacccg cagccattgc cgcccgtcgc ggcgacgacg
     3661 gtggtcgacg gatacacggt ggcgctgagc ggagcaccaa aggccaatga gccttcgatg
     3721 ttgacgttgg cggttagccg taacggcaag ccggtcaccg acttgcagcc gtatctgggt
     3781 gcctatggcc acctggtggc gttgcgtgcg gcggatctcg gatacctgca tgtccatccg
     3841 atgggtgagc ccggcgacgg catcaccgcc gctgggcccg agatcgcgtt ccacgcaacc
     3901 tttccgagtg cgggcgccta ccggctgttc ctcgatttcc agcatgatgg agtggtgcgc
     3961 accgcagcgt tcaccgtgac cgtcgaggat ggagaagcga catgacgtcg gtcgagctct
     4021 cgatcgacgg catgacctgc gcgtcgtgcg cacatcgaat cgagaagaaa ctcaacaaga
     4081 tcgatggggt gaccgcgacc gtcaacttcg ctacggagaa ggcccgcgtc gagtacggca
     4141 ccgaggtcac acccgaacag ttggtggcga cagtcgatga ggcgggctac caggctcatt
     4201 tgcccgagga gcctgtcgaa gccgcggccg agcaagatcc gaccgcctcg ctgctgcaac
     4261 gactgctgat ctccgcggcg ctgacgattc cggtgatcgc aatggcgatg gtgccggccc
     4321 tgcagttcac gtactggcaa tggctgtcgc tggcgctcgc cgcacccgtc gttgtttggg
     4381 gagcatggcc gttccaccgg gcggcgtggg cgaacctgaa gcacggcacc gcgacgatgg
     4441 acacgctgat ctcgatgggc acgctcgccg cgttgggctg gtcggtctac gcgttgttct
     4501 ggggcaccgc gggcatgccg gggatgaagc atgcgttcga gctgacgatt gcccggacgg
     4561 acggaaccgg caacatctat ctggaagccg ctgcgggagt gaccacgttc atcctcgccg
     4621 gacgctattt cgaggctagg gccaaacgcc gggcgggtgc ggcgctggag gcgctgctgc
     4681 atctcggcgc caaggatgtc tcggtgctga gaagcggtgt cgagcaacgt attccgattg
     4741 accagttgtc cgtcggcgat gagttcgtgg tgcgtccggg cgagaagatc gcgaccgacg
     4801 gcgtcgttgt cgacggcaat tcggcagtgg acgcgtcgat gctgaccggt gagtcggtgc
     4861 cggtggatgt ggggccagac gaccaggtgg tcggggcgac ggtcaatgtc
//
LOCUS       DASRJA010000733         8761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_668273,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000733 DASRJA010000000
VERSION     DASRJA010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668273"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="VFC01_33125"
     CDS             complement(<1..1418)
                     /locus_tag="VFC01_33125"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN84498.1"
                     /translation="MWRRRPSTAAFAFAANVMGGLVHSARSLAAVADGVAASYMSRTA
                     TLEPRMTPTKLDDLAAQARRDFARMNFPAANWVPPTSGPDGQPMLDVLVIGAGMCGQT
                     AAFALMREGVRNLRLVDRAERGREGPWGTYARMDTLRSPKHLTGPDLGFPALTFRAWY
                     EAQHGAEGWQRLYKVATRDWLDYLLWVRDTVEIAVENGTEVTRLDPGSDAVRVELVRA
                     GRRETIYTRKVVLAGGRDGSGAPHWPAFPSLPDRRQAGDHVFHSSDAIDLARFKGGRV
                     GVLGASASAFDNAAVALESGAAEAHLFVRRPHLPQINKSKWTVFPGFLHGYPRLDDRA
                     RWQIYTYIFCEAVPPPHESVLRCDRHPGFAMHFAEPWNDVLPCPDAVKVITARTEYRF
                     DAVILATGFAVDLRLRPELAHFSDKIALWADRVSAEAAALHPAEARHPYLGPGFELTE
                     RAGGSMPGLSHLYCYNAGSTMS"
     gene            1475..2941
                     /locus_tag="VFC01_33130"
     CDS             1475..2941
                     /locus_tag="VFC01_33130"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HZN84499.1"
                     /translation="MANFAYGGLRQSYAALWKAMQIPTGVRVQATDARVRAIAEAKPR
                     YDQVANATGVPWYVIGIIHEMEGGLNFSTHLHNGDPLTARTVQVPAGRPPTGVPPFQW
                     EESAIDALTLEAFHSVKLWTIERIAYQLEAYNGWGYRSRNTGVNTPYLWSATNNYQKG
                     KFVRDGVFDANAVSGQIGAMALLRRLIDVIGINVPAEDDVPLPDTPSLPTEPDLGTQP
                     YPGMAVRLNHPDPRIVMLLQRRLNQLGCWPSIDAKGKTVPLNVDGDFGQLTASAVKLF
                     QARSQDGEGKPLSVDGVVGLTTWTALFGTPSVAPVTASVPAQGLLRRVITVAANEEQQ
                     QVREQPLGSNRGPRVDQYLRAAGIDPTTGSYPWCAGFIVWCFDNAARQLGLASPVPRT
                     AGVHDMWQKAGRAGFRRISHETAIGDPSQVQPGQVFFIDTGQGHGHTGLVVGREGSEF
                     QTIEGNTNELGGREGVGVFARRRPVRQATLGFVEFAAR"
     gene            3035..3283
                     /locus_tag="VFC01_33135"
     CDS             3035..3283
                     /locus_tag="VFC01_33135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84500.1"
                     /translation="MQEEVALMHEVYDRERAAALVKEARDMIAALKCETGEIAVQMSR
                     VRHDPDDGDAPHQRLPKATLAPSIQRTPNRKPFWKFWS"
     gene            3328..3453
                     /locus_tag="VFC01_33140"
     CDS             3328..3453
                     /locus_tag="VFC01_33140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84501.1"
                     /translation="MRDKLTWILVFAFIAAGGSTYSAYKVYQMTDGRPAPTKNKR"
     gene            3546..3998
                     /locus_tag="VFC01_33145"
     CDS             3546..3998
                     /locus_tag="VFC01_33145"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZN84502.1"
                     /translation="MKVADILERKGSVIITIKPTETIAALSQRLRERRIGAAIVSNDG
                     QSVDGVISERDIAYGLCVHKGELHAMPVSALMTKAVITCAPSDELGIVASTMLSRNVR
                     HLPVMDGERLIGMVSIRDVLNFRVDQLQRETGALRAFAMQTDREAQDR"
     gene            complement(4089..4307)
                     /locus_tag="VFC01_33150"
     CDS             complement(4089..4307)
                     /locus_tag="VFC01_33150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84503.1"
                     /translation="MFAPGFGNDKITDFDADPSGGQDLIDLSALDIAYDDLSVTAVSG
                     GVKITYEEDSITLKGAKLSSISSSDFIF"
     gene            complement(4509..>8761)
                     /locus_tag="VFC01_33155"
     CDS             complement(4509..>8761)
                     /locus_tag="VFC01_33155"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HZN84504.1"
                     /translation="LLGLPLDLATVNLARGRDTGVPTLNAARREFYNGTGDSQLKPYT
                     SWVDFAINAKNPLSVVNFIAAYGTHQSILDAGTAAEKRAAAELLVFGGAGAPADRLDF
                     LNSTGAWANLANGVTTSGVDAIDFWIGGLAEKQMPFGGLLGSTFNFVFETQLEALQNG
                     DRFYYLTRTAGLNFLTQLEQNSFASMIMRNTDATHLPADVFSTPAFILEVDQTRQFNE
                     GLGSDDPLGDNPFIALVVRDNPQTAGVDTNYLHYTGEDHVVLGGTEGNDTMISGIGDD
                     TLWGDGGNDRLEGGDGADQILGGDGDDILTDLGGDDIIQGGDGNDVINGGNGINLLLG
                     GFGKDFIMAGEDLSEIFGGDGDDFLYGSPTTEAVIGNEGSDWIETGTADGSPGDNFDP
                     NALDLIDGHDVFIGSGGVDEFMGEGGDDIMVGGPGADRMEGMSGYDWVTYKNDTLGVT
                     VDLTLEAFDETPIPPSNAAVMDRFDQLEGLSGSDFSDILLGDDADAVEIQAAGNRGSV
                     LTSEGIARIAGLQDILGAGVTSFDGGNIILGGGGSDIIQGRGGNDIIDGDALLNVRIG
                     IFDEAGNEIGTANSMVGPVTSSLPALNGQQLSALMLAGTVNPSQLQIVREILMTGGQN
                     DVDTAVFSGPRANYQIGEPDAEGRRVIIDTVGTDGVDIIRNIERLQFADEIVDLVNTG
                     NSPPQGQLEILGLPATEDSVLTATLGTATDPDNPNGGIITGSIAYVWQMEEDAGTGIF
                     VDILRADEFGQDVAASGPTFTPSNDEVGLLIRVKATYLDADGVIETVFSAPTGAVINV
                     NDAPTGAPVISDASPTEGLALSVSTASIIDLDGIDPAVVFTFQWQELIGSNWTDIAGA
                     TDGIFVPQQDQVGRQLRVAVTYTDDQGTTETVLSAATGIVGDEIFSDVGAETINGTEG
                     DDVIFSGLGIDIVNGLGGNDEIHGEAAGDTLNGGDGNDLIFGEGGADQISGGNGNDTL
                     DGGVAGDTINAGDGNDTITGGAGADVLQGGNGNDLFLYTMGEGADSVDGGAGTDTLRI
                     LGTDGAETLNVVFNGTALTTVENGTVTGVEAVTADLLGGVDTLTYAGSTAAVTVDLAA
                     NSASGFLSLRDVENVTGGGGADTLTGSVAANVLNGGGGNDTLSGGGGDDTINGGGGND
                     TIIWSAGDGRDIINGGAAAGTDTFVLNGNAEVEEFRIETSAAYLIRVPGAALAATTQI
                     VVSRNGVVAAELANIDEISVNSGAGDDTFIIAGSFTGTDLNLNTITIEGSSGNDTVDV
                     SGRGSEHRVVFKSNGGNDTFIGPMNSGDVVELLPGTTVNETTVTNNGDGTCTIESGTQ
                     KFTANSSAAVFQGLGNDNPDPGEDPDPGEEPTPDPTIYGTEGRDCLNGTDGDDHIAGL
                     EGRDKIYAGKGDDILDGGTGYDRLHGGEGDDLYIVDHC"
BASE COUNT         1459 a         2850 c         2877 g         1575 t
ORIGIN      
        1 tggctcatgg tcgagcctgc gttgtagcag tacaggtgcg acagtcccgg catcgaccca
       61 cccgcccgct ccgtcagctc gaagccaggc ccgaggtatg ggtgtcgcgc ctccgccgga
      121 tgcagggccg ccgcttctgc cgaaacgcga tcggcccaca gcgcgatctt gtcggagaaa
      181 tgcgccagct cgggtcgcag ccgcaggtcc accgcgaatc cagttgcgag gataaccgca
      241 tcgaacctgt attcagtgcg tgccgtgatg actttgaccg cgtcggggca gggcagcacg
      301 tcgttccacg gttcggcgaa atgcatcgcg aagcccgggt ggcggtcgca gcgcagcacc
      361 gactcatgcg gcggcggcac cgcttcgcag aagatgtagg tgtagatctg ccaccgcgcc
      421 cgatcgtcga ggcgcggata gccatgcaag aagcctggaa acaccgtcca cttcgacttg
      481 ttgatctgcg gcagatgcgg ccggcgcacg aacagatgtg cctcggccgc gcctgactcc
      541 agggcgacgg cagcattgtc gaaggcggag gcgctggcgc ccaggacacc gacccggccg
      601 cccttgaagc gggccaagtc gatggcgtcg gaggaatgga acacgtggtc gcccgcctgg
      661 cgccggtcgg gcagcgaggg aaaagccggc cagtgcggcg cgccggatcc gtcgcggccg
      721 cccgccagca ccaccttgcg ggtatagatc gtctctcgcc ttcctgccct caccagctcg
      781 acacgcaccg catccgagcc gggatcgagc cgcgtgacct cggtgccgtt ctcgacggcg
      841 atctccaccg tatcgcgcac ccacagcagg tagtcgagcc agtccctggt ggcgaccttg
      901 tagagccgct gccatccctc ggcgccgtgc tgcgcctcgt accaggcgcg aaacgtcagc
      961 gcgggaaagc cgaggtccgg ccccgttagg tgcttgggcg agcgcagcgt gtccatgcgc
     1021 gcgtaggtgc cccacgggcc ttcgcggccg cgctcggcgc gatcgaccag gcgcaggttg
     1081 cgcacgccct cgcgcatcag ggcaaaagcc gccgtctggc cgcacatgcc agcgccgatc
     1141 accaggacat cgagcatcgg ttggccgtcc ggccccgacg tcggcggcac ccagttggcg
     1201 gcggggaagt tcatgcgcgc aaaatcgcgc cgggcctggg cggcgagatc gtctagcttg
     1261 gtgggcgtca tgcggggctc gagcgttgcg gttcttgaca tatagcttgc ggcaaccccg
     1321 tcagccacgg ccgccaggct acgtgcggaa tgcacgagcc cgcccatgac attggccgca
     1381 aaggcaaaag cagcggtgga tggacgacgg cgccacactt ccgcctcgcc ccgactcagt
     1441 taccgtccgc agccgaccgg gcgaggggga agtcatggcc aattttgcct acggcgggct
     1501 gcgccaatcc tatgcagcgc tgtggaaagc aatgcagatt ccgacggggg ttcgggtgca
     1561 ggccaccgac gcccgcgtgc gcgccatcgc cgaggccaag ccgcgctacg accaggtcgc
     1621 caatgcgacg ggcgtgccct ggtacgtaat cggcatcatc cacgagatgg aaggcgggtt
     1681 gaacttttcc acccacctgc acaatggcga cccgctgacc gcccgcacgg tgcaggttcc
     1741 ggccggccgg ccgccaaccg gcgttccacc cttccagtgg gaggaaagcg ccatcgatgc
     1801 gctgacgcta gaggcgtttc attccgtcaa gctgtggacg atcgagcgta tcgcctacca
     1861 gctcgaggcc tacaacggtt ggggctatcg ctcgcgcaac accggcgtca atacgccgta
     1921 tctgtggagt gccaccaaca actatcagaa gggtaagttc gttcgcgacg gggtgttcga
     1981 tgcgaacgcc gtcagcggac agattggcgc catggcgctg ctgcgccgcc tgatcgacgt
     2041 catcggcatc aacgtgccgg ccgaggacga cgtccccctt cccgacacac catccctgcc
     2101 aaccgaaccg gatctgggca cgcagcccta cccgggcatg gccgtgcgcc tcaatcaccc
     2161 cgaccctcgc atcgtgatgc tgctgcagcg gcggctcaat cagctcggtt gctggcccag
     2221 catcgatgcc aagggcaaga ctgtgccgct caacgtggac ggcgacttcg gacagctcac
     2281 ggcatccgcc gtcaagttgt tccaggcgcg ctcacaggac ggtgagggca agcccctgtc
     2341 ggtcgacggc gtggtcgggc tgaccacctg gacggcgctg ttcggcacgc cgagcgtcgc
     2401 tccggtcacc gccagcgtgc ctgcgcaggg cctgctacgg cgggtgatca cggtggccgc
     2461 caatgaggag cagcagcagg tgcgcgagca gccgctgggc tccaaccgag ggccgcgcgt
     2521 cgaccaatat ctgcgcgccg ccggcatcga tccgacgacc ggcagctacc cttggtgtgc
     2581 gggcttcatc gtctggtgtt tcgacaacgc ggcgcgccag cttgggcttg cctcgccggt
     2641 gccgcgtacg gccggcgtgc acgacatgtg gcagaaggcc ggccgggcgg gtttccggcg
     2701 catctcacac gaaaccgcaa tcggtgatcc gagccaggtg cagccaggcc aggtcttctt
     2761 catcgacacc gggcagggcc acgggcacac gggtttggtc gtcggccgcg aaggcagcga
     2821 gttccagact atcgagggta ataccaacga gctgggcggc cgcgaaggtg ttggcgtgtt
     2881 cgcgcgccgg cgcccggtgc ggcaggcgac cctggggttc gtggaatttg ccgcgcgctg
     2941 acgattgatt gccgcaatct gaccgcgaat cgtcaggtag cgtctcactt gatttcggtg
     3001 cgttgccacg agccccacgc ccacggcgtg gacggtgcag gaggaggtcg cgctgatgca
     3061 tgaggtctac gaccgggaaa gagccgccgc gctggtcaag gaggcgcgtg acatgatcgc
     3121 cgccctcaaa tgcgagaccg gagagattgc ggtgcagatg agccgcgtgc ggcacgatcc
     3181 ggacgacggt gatgcgccac accagaggct gccgaaggca acacttgccc cctccattca
     3241 gcgcacaccg aaccgaaagc cgttctggaa gttctggagc tagcgcagcc gatgttcgca
     3301 aactgcacgt tcccggcagg aggctagatg agagacaagc tgacctggat tttggtgttt
     3361 gcgttcatcg ccgcgggcgg cagtacctac agcgcctaca aggtctacca gatgacggac
     3421 ggcaggcccg cccctacgaa aaacaagagg tagacgtctg ttattgacga cggcggttgc
     3481 cggcagcggc cggattggca aagataggtg cctcttacag gtaggatctt cgctggagta
     3541 gtaccgtgaa ggtagccgac attctggaac gcaagggctc cgtaatcatt accatcaagc
     3601 cgacggagac gattgcggcg ctgtcgcagc gcctgcgcga acgccgtatc ggcgcggcca
     3661 tcgtctccaa cgatggacag agcgtggacg gagtaatatc cgaacgcgac attgcctatg
     3721 gcctgtgcgt gcacaagggc gagttgcacg ccatgccggt ttcggcgctg atgaccaagg
     3781 ctgtgatcac gtgcgccccc agcgatgagc tcggcatcgt agcctccacc atgctgtcgc
     3841 gtaatgtgcg gcacctgcct gtcatggacg gcgagcgcct gatcggcatg gtgagcatcc
     3901 gcgacgtgct gaacttccgc gtcgaccagc tgcagcgcga aaccggcgcg ctgcgtgcct
     3961 tcgcgatgca gaccgaccgc gaggcgcagg accgctagtg cgcgcgctcg cgcaaaccaa
     4021 gccagtcccc taacaaaaaa atgcagccct ggatttccca aggccgcatt ttttcttgcc
     4081 ttgcggcttc agaagatgaa gtcgctgctg ctgatggacg acagcttggc gccctttagc
     4141 gtgatggaat cttcctcgta ggtgatcttc acaccgcccg acacggccgt cacagaaagg
     4201 tcgtcgtagg cgatatcgag ggccgacaga tcgataagat cctgaccgcc tgacggatcg
     4261 gcgtcgaagt ccgtgatctt gtcgttgccg aagcccggcg cgaacacgaa tgtgtcgttg
     4321 ccgccggcgc ccgtcagcca atcgtcgctg gagccgccct cgatgacgtt gtcgagcttg
     4381 ttgccgacac ccttgaaagc gccgtcgccc gtatagatca ggttctcgac attgtcgtcg
     4441 agccagtaga agcggaggct ggtctcgacc gtatccgtcc cgccgttccg atcctcccgg
     4501 atgtcatctc agcagtggtc gacgatgtat aggtcgtcgc cttcgccgcc gtgaagcctg
     4561 tcatagcctg tcccgccgtc gaggatgtcg tctcctttgc cagcgtagat cttgtccctg
     4621 ccctcaaggc cggcgatatg gtcgtcgccg tcggtgccat tcaggcaatc acgcccttcg
     4681 gtaccgtaga tggtcgggtc cggtgtcggc tcctcgcccg gatcgggatc ctcacccgga
     4741 tccgggttgt cgttgcccaa gccttggaaa accgcggcgc tggagttggc cgtgaatttc
     4801 tgggtgccgg attcgatggt gcaggtgccg tcgccgttgt tggtcacggt cgtctcgttg
     4861 accgtggtcc cgggcagcag ctcgacaacg tccccactgt tcatggggcc gatgaacgtg
     4921 tcgttgccgc cgttcgactt gaacaccacc cggtgctctg atccgcggcc ggacacgtcc
     4981 acggtgtcgt ttccgctcga gccctcgatg gtgatggtgt tcagattgag gtcggtgccc
     5041 gtgaagctgc ccgcaatgat gaacgtgtcg tcaccggcac cgctgttgac cgagatctcg
     5101 tcgatattgg caagctctgc ggcgacgacg ccgttgcgcg aaacgacaat ctgcgtggtg
     5161 gcagcaagcg cggcgccagg gacgcgaatc aggtaagccg cgctggtctc gatcctgaac
     5221 tcctcgacct cagcgttgcc attgagaacg aacgtgtcgg tgccggcggc ggcaccgccg
     5281 ttgatgatgt cgcgtccgtc gccggccgac cagatgatcg tatcgttgcc gccgccgccg
     5341 ttgatggtgt cgtcgccgcc gccgccgctc agggtgtcgt tgccgccacc gccgttcaac
     5401 acgttcgctg caacgctacc ggtgagagtg tcggcgccgc cgccgccggt gacgttctcg
     5461 acgtcacgca gcgagaggaa gccagacgcg ctgttggccg ccaggtcgac cgtgaccgca
     5521 gccgtgctgc cggcataggt cagtgtgtcg acgccgccga gcaagtcggc cgtcaccgcc
     5581 tcgacaccgg tgaccgtccc gttctcaacc gtggtcagcg cggtaccgtt gaagaccacg
     5641 ttgagcgtct cggcgccatc ggtcccgagg atgcgcaggg tgtcggtgcc ggcaccgccg
     5701 tccaccgagt ctgcaccctc acccatcgtg tagaggaaca gatcattgcc gttgccgccc
     5761 tgaagcacgt ccgcgcccgc accaccggtg atggtgtcgt tgccatcgcc agcattgatg
     5821 gtgtcgccgg ccacaccgcc atcgagcgtg tcgttgccgt tgccgccgga gatctgatcg
     5881 gcgccgccct caccgaagat gaggtcgttg ccgtcgccgc cgttcagggt gtcgcctgca
     5941 gcctcgccgt gaatctcgtc gttgccgcca aggccgttga cgatatcgat ccccaagccg
     6001 gagaagatga cgtcgtcacc ctccgtgcca ttgatggtct ctgcgccgac atcggagaag
     6061 atctcgtcgc cgacgatgcc ggtcgccgcc gagagcacgg tctccgtcgt gccctggtcg
     6121 tcggtatagg tcaccgccac gcgcagctgc ctgccgacct ggtcctgctg cgggacgaag
     6181 atgccgtcgg tcgcgccggc aatatcggtc cagttcgaac cgatcagttc ctgccactgg
     6241 aaggtgaaga cgacggccgg atcgatgccg tcgaggtcga tgatgctggc cgtcgatacg
     6301 cttagcgcca gcccctcggt gggactcgcg tcgcttatga ccggcgcgcc ggtcggtgcg
     6361 tcattgacgt tgatgacagc cccggtcggt gccgagaaca ccgtctcgat gacgccgtcg
     6421 gcatcgaggt aggtcgcctt cacacggatc agcaggccga cctcgtcgtt gctcggcgta
     6481 aaggtcggtc ccgatgcagc cacgtcctgg ccgaactcgt ccgcgcgcag gatgtcgacg
     6541 aagatgcccg tccccgcatc ctcctccatc tgccacacgt aggcgatcga cccggtgata
     6601 atgccgccgt tgggattgtc ggggtcagtt gccgtcccca gcgtggctgt gagcacggag
     6661 tcttcggtgg cgggcagccc cagaatctcg agttgcccct ggggcgggct gttgccggtg
     6721 ttcaccaggt cgacgatctc gtcagcgaat tgcaggcgct cgatgttgcg gatgatgtcg
     6781 acgccgtccg tgccgaccgt gtcgatgatg acccggcggc cctcagcatc gggctcgccg
     6841 atctgatagt tggcgcgcgg accggagaac actgccgtgt ccacatcgtt ctgcccgccg
     6901 gtcatcagga tttcgcgcac gatctgcagc tggctcgggt tgaccgtgcc cgccagcatc
     6961 agcgcgctca gctgctggcc gttcagcgcc ggcaggctac tggtgaccgg gccaaccatg
     7021 ctgttggcgg tgccgatctc gttgccggct tcatcgaaga tgccgatgcg cacgttgagc
     7081 aatgcatcgc cgtcgatgat gtcgttgccg ccgcggccct ggatgatgtc gctgccgccg
     7141 ccgccgagga tgatgttgcc gccgtcgaac gaggtgacac cggcgccaag gatgtcctgc
     7201 agcccggcga tgcgggcgat gccctcgctg gtgagcacgc tgccgcggtt gccggcggcc
     7261 tggatctcga cggcgtcggc atcgtcaccc agcaggatgt cgctgaagtc ggagccggac
     7321 aggccttcca gctggtcgaa ccgatccatc accgccgcat tggagggcgg gatcggcgtc
     7381 tcgtcgaagg cttccaaggt caggtcgacg gtgacaccca gcgtatcgtt cttgtaggtg
     7441 acccagtcgt agcccgacat gccttccatg cggtcggcgc cggggccgcc gaccatgatg
     7501 tcgtcaccgc cttcgcccat gaactcgtcg acgccgccgc tgccgatgaa cacgtcgtgt
     7561 ccgtcgatca gatcgagcgc gttcgggtcg aagttgtcgc ccggcgagcc gtccgcggta
     7621 ccggtctcga tccagtcgct gccctcgttg ccgatcacgg cctcggtggt cgggctgccg
     7681 tataggaagt cgtcaccgtc gccgccgaag atctccgaga gatcctcgcc cgccatgatg
     7741 aagtccttgc cgaagccgcc gagcagcagg ttgatgccgt tgccgccgtt gatgacatcg
     7801 ttgccgtcgc cgccctggat gatgtcgtcg ccacccagat cggtcaggat gtcgtcgccg
     7861 tcgccaccga ggatctggtc ggcgccgtcc ccgccttcca accggtcgtt gccaccgtcg
     7921 ccccagaggg tgtcgtcgcc gatgccggag atcatggtgt cgttgccctc ggtaccgccg
     7981 agaacgacgt ggtcctcacc tgtgtagtgc aggtagttgg tatcgacacc cgctgtctgg
     8041 ggattgtcgc gcaccaccag ggcgatgaag ggattgtcgc ctagcgggtc gtcgctgccg
     8101 aggccttcgt tgaactgcct ggtctggtcc acctcgagga taaaggccgg ggtcgagaac
     8161 acgtcggccg gcaggtgggt ggcgtccgtg ttgcgcatga tcatgctggc aaacgagttt
     8221 tgctcaagct gggtgaggaa atttaggccg gccgtgcgcg tcagatagta gaagcggtcg
     8281 ccgttctgca gtgcctcgag ctgggtctca aacacgaagt tgaaggtcga gccgagcagt
     8341 ccgccgaacg gcatctgctt ctcggcaagc ccgccgatcc agaagtcgat ggcatcaacg
     8401 ccagacgtag tcacgccgtt ggcgaggttg gcccatgctc ccgtgctgtt caggaagtcg
     8461 aggcgatcgg cgggggctcc cgcaccgccg aacaccagca gctcggctgc cgcgcgcttc
     8521 tcggccgcgg tcccggcatc caggatgctc tggtgggtgc cgtaagcggc aatgaaattg
     8581 accaccgaga gcgggttctt cgcgttgatg gcgaagtcga cccagctggt atagggcttg
     8641 agctggctgt cgccggtgcc gttgtagaac tcgcgccgcg ccgcgttcag tgtcggcacg
     8701 cccgtgtcgc ggccgcgggc caggttgacg gtggccaggt cgagcggaag gccgagcagg
     8761 t
//
LOCUS       DASRJA010000734        27285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3770647,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000734 DASRJA010000000
VERSION     DASRJA010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 27285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27285
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3770647"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFC01_33160"
     CDS             <1..266
                     /locus_tag="VFC01_33160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN84505.1"
                     /translation="QVRELAALTRSSPVLSAWTLQIYLRYEAAIADLVAPRLGSAAPD
                     DPRPRLLGALAMASIRIALDDWLAHGGSLPARVRTALSSVRIE"
     gene            complement(267..713)
                     /locus_tag="VFC01_33165"
     CDS             complement(267..713)
                     /locus_tag="VFC01_33165"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN84506.1"
                     /translation="MTIDEVDEKFATEFADRFAETWRTPDLAKHEALWSDDIVLIQPM
                     MGTLRGKHACRESFQRLFALVPDLHADVHRVGHGKHEVFIEFTLSGTYGGKPIAWDAV
                     DRFTFTNGLIAERVSYFDSAPLLGKLVARPAGWDRLVRIGSQLFRR"
     gene            complement(814..1200)
                     /locus_tag="VFC01_33170"
     CDS             complement(814..1200)
                     /locus_tag="VFC01_33170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZN84507.1"
                     /translation="MRTTEGHLLGQMGMRDVVDTDERLIIEMDNRPDLTNIRGALQGG
                     LVATLIDIAAGRLAEHHVGPDQSVTTADMNVHFLAPVVVGPARAEATIVRAGKRLVVT
                     AVDVTDVGRDRLAARATLSFAVLDPR"
     gene            1286..2143
                     /gene="map"
                     /locus_tag="VFC01_33175"
     CDS             1286..2143
                     /gene="map"
                     /locus_tag="VFC01_33175"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZN84508.1"
                     /translation="MPVRTALRPGVLSPTLPVPRTIAPPEYAWKPTVKEGSEPWVQTP
                     EVIAKMRISGRIAAGALAEAGKAVAPGVTTDELDRIAHAYMIDHGAYPSTLGYKGFPK
                     SCCTSLNEIICHGIPDSTVVEDGDIVNIDVTAYIDGVHGDTNATFLAGDVSEEHRLLV
                     ERTQEATMRAIKAVKPGRQLSVVGRVIESYANRFGYNVVRDFTGHGIGTTFHNGLVVL
                     HYDQPAVETVLEPGMTFTIEPMINLGALDYEIWDDGWTVATKDKKWTAQFEHTLVVTD
                     DGAEILTQL"
     gene            2223..3704
                     /locus_tag="VFC01_33180"
     CDS             2223..3704
                     /locus_tag="VFC01_33180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HZN84509.1"
                     /translation="MTPSGALLVAGTTSDAGKSMVVAGLCRLLARKGIRVAPFKAQNM
                     SNNSAVTVEGGEIGRAQAMQARAAGLAPSVRFNPILLKPGSDRTSQLVLRGHVVDTVS
                     AHDYFTHRDRLAAVVADELRQLRSEFDAVICEGAGSPAEINLRATDLANMGLARAAEL
                     PVILVGDIDRGGLLAHLYGTVAVLEPDDQRLISGFVVNKFRGDPSLLAPGLEQLEALT
                     GRPTYGVIPYTDGLWLDTEDSVSVLAHRVVGDPQPPRGADWLRVAAIRLPRISNSTDI
                     EALACEPGVVVRWVTDPAHLADADVVVIPGSKSTVADLTWLGEKGLADGITVHARQNK
                     PVLGICGGFQMLCKTIDDPVESKAGVVEGLGLLDADIVFAPDKTLRHHDSPLCGYEIH
                     HGQVARHGDGDWHGVGIRSGRVYGTHWHGLLDNNDLRRQWLTEVGTPGFVVADDVDVA
                     ARRDAQLDLMADLLTAHLDVDAVVAVLESGPPQRPMIVTGLRH"
     gene            complement(3685..4719)
                     /locus_tag="VFC01_33185"
     CDS             complement(3685..4719)
                     /locus_tag="VFC01_33185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZN84510.1"
                     /translation="MATMRDVAGRAGVSAKTVSRVVNNDRYVSADVRERVERAIEELQ
                     YVPNMLAVTFRSGRDAAIGVAVPGVADPFFASIIGAVEREASRRGVAVIVTAVGWEPS
                     HERQSIEAVLKRQVAGMIICPVGRDMSYLQPWQKRTPLVFVDREPGRLTADTVLQDDV
                     GGGHQAGRHLISHGHRSIAFMGDDTYTGLLRLKGLQNALDEAGLPQRSDLTHVGAIDA
                     GTLTAELRRMLAASDPLSAVFSSNARVTIALVTALQALRRKDIGLVGFGDFPTAAALK
                     PAVTVIHQDGDEMGRFAADRLFTRLDQPTRRLRRRTVLPVSLVTRTSCAMPGEKVRAG
                     HQLVVSGAAP"
     gene            4894..5931
                     /locus_tag="VFC01_33190"
     CDS             4894..5931
                     /locus_tag="VFC01_33190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HZN84511.1"
                     /translation="MQPQLLPPNVVPHWYAGGSALAAWRGLPSVGERSPEEWVGATVA
                     RFGAPNLGPARLADGTLLRDAVSADPLGWLGRDDAEPGDTGVLVKLLDAGQRLPVHVH
                     PTREYAFQHLGCAYGKTEAWFVLEADDGAAVWVGWREEVGRDRLMKLVEAQDAEAMLA
                     LMHRVPVRPGDGVLVPGGAPHAIGAGILLVEAQEPTDQSILLERTNTTASDDEIFLGL
                     TREVALSVVETTARTNVSALTRHTDKHVAGLVQVLTEQAEPFFRMALLTAGVDMPAGF
                     AVAVVLSGAGTLAAAHSETVDVERGQTLVVPASSGDWHLDGDVQLLVCRPGTTWPPKP
                     TPNIKQQEGAA"
     gene            5928..6692
                     /locus_tag="VFC01_33195"
     CDS             5928..6692
                     /locus_tag="VFC01_33195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN84512.1"
                     /translation="MTALLEARAVSRSFGHVRALDRADFDISPGEVVGLIGDNGAGKS
                     TLIKALSGSLELDEGEIYFDGKPVRLSSPRQANDLGIEVVYQDLALAPHLNPVQNVFL
                     GREIPAKGVLGHLGFMDEKEMRRQAAASFRALGATVRSLSSPVGSMSGGQRQGIAIAR
                     AIAWADKVVILDEPTAALGVVQTKNVLESVKRVRDKGIAVVFISHSMPHVLEVCDRIQ
                     VLRLGRRVATYRGRSTTVETLVGAMTGALDAKDGGA"
     gene            6765..7838
                     /locus_tag="VFC01_33200"
     CDS             6765..7838
                     /locus_tag="VFC01_33200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN84513.1"
                     /translation="MSTVANQQDAVADRADELDSAQSLLKRVVGLQAFWILGVLIVIC
                     LFFTVIAGDRFLSTGNFSLISQNVAVWAVLGVGMTFVIITSGIDLSVGSVLVFSSVVA
                     AKVMEAMGGSGIGVAAVGIVAAILSGMAWGVVNGALVAIAKVPALIVTLGTLSVALGL
                     AQVLTGGIDIRSVPSELTDFSAYTKILGIPGLPFVALIVIVLGGILLHKTRFGRYTYA
                     IGSNEEAARRTGIKVSRHLVLVYGLAGTLAGLGALLSLAQYGTTTIAGQSLTNLNVIA
                     AVVIGGTSIFGGYGTIFGTVVGLFIPAVLQSGFVIIGVQPFWQGVAVGSVLIAAVYVD
                     QTRRAAAMRSARSRGLFKRKGSK"
     gene            7850..8899
                     /locus_tag="VFC01_33205"
     CDS             7850..8899
                     /locus_tag="VFC01_33205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN84514.1"
                     /translation="MNRKRVMPVAGALGAVVLTLSMASCTSSKPQSSGSGETSNGVTP
                     AAAATTVTAPPKASKNYNIQLLQGVIGDQFYITMQCGAQEEAAKLGVTLNSQGPQKFD
                     PTLQKPVLDSIVAARPAALLVAPTDVQAMQQPLEQAAAAGIKVVLVDTTTNDPSYAVS
                     SIASDNEGGGKAAFDAIKQLHPEGGKVMVMNVDPGISTTDARAKGFEDAVKADGKFTY
                     VGVQYSHDDPATAAQLIGAQLQKDPDLVGVFATNIFSAEGSATGVKQAGKSGQVKIVG
                     FDAGPNQIQALRDGTVQALVAQEPGIIGKFGVDEAVTALDGGQNTKKVQTGFTIITQE
                     NLNGEGGAAAYKSSC"
     gene            complement(8901..9551)
                     /locus_tag="VFC01_33210"
     CDS             complement(8901..9551)
                     /locus_tag="VFC01_33210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZN84515.1"
                     /translation="MTGSDAVNLRVDGLLLDCDGVLVDSHNAAAVAWNQWAQRWAPGF
                     DFHRDIEHGRRIRDVVAELISTPSDVAEAAADLIQLELDHAADVVPIPGARELLESIP
                     QGSWAVVTSGGRTIATARMVSAGLVPAAVLVTGEDVERGKPFPDPYLLAARRLGLSPQ
                     RCAVFEDAPAGIAAAQAAGVETIIGVGTAATAGVTVAVADLRGVRFDGQRLQISSM"
     gene            9576..10304
                     /locus_tag="VFC01_33215"
     CDS             9576..10304
                     /locus_tag="VFC01_33215"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN84516.1"
                     /translation="MADFAGRGVLVTGASGGIGAATVRRLVSQGATVYAGGRNVEQLT
                     KLCDETGAAPVPFDLTSEDEIRAAVSGLDLWGVVNCGGFGGEIATPQDTDIAVFDKVI
                     SINARGSLLVIKHATPAMITRGVGGSIVNVSSQASLVALSGHISYGSSKAALDNITRV
                     AALELGRHNIRVNSVNPTVVMTDMSAFYWGRPDVGPPFLEQMPLGRWATEDEIAAPIC
                     FLLSDDASMITGVTLPIDGGYSCR"
     gene            10345..10995
                     /locus_tag="VFC01_33220"
     CDS             10345..10995
                     /locus_tag="VFC01_33220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84517.1"
                     /translation="MIRRAVVALLFVALTGCSHAISGTATWPGARLETVVLTAADFPT
                     GVQYDRLTRAPGQPDGAGGPPAMLSNPEGCSDGLTRAIADSAERGPGSAAEYVVGYDG
                     ARIVMTVLTWPLDLDRLAAIADRCAHFQTFFDPTAPGIPMTTTRLATPRHDALVYQQT
                     MRLNNVDNSVYFTFENIGTMAVFGIAFPTPNPSITVKATLPQTFLDIAAKQADRAR"
     gene            11047..11610
                     /locus_tag="VFC01_33225"
     CDS             11047..11610
                     /locus_tag="VFC01_33225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN84518.1"
                     /translation="MPSTMVTVDGFPVPVDVAGPDKGSVVVVLGAAHHATAAYEAVCQ
                     RLHTASLRTIVIGPDPRLTAKSVVGILDALDVRWALLVGDRIGGELAWELAATRLDRF
                     IGLVVIDRGHPRVADLTGQIRDEHCPPVEMNTTALVSTPAARAVAKASQRYVYGDFRL
                     VDLLGRRNAQESTAQLAAEIVMRTSTW"
     gene            complement(11624..13387)
                     /gene="ngg"
                     /locus_tag="VFC01_33230"
     CDS             complement(11624..13387)
                     /gene="ngg"
                     /locus_tag="VFC01_33230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine synthetase"
                     /protein_id="HZN84519.1"
                     /translation="MTATDPAQDHSEAITLGLHDASPQHMVDAMADDVVVELGWGRLI
                     FGQTFADPNKLAEVLRQEGQGRRDICMYAREPQVLVALAPAELFIDPSHTYRLRFSED
                     ELDLSPTGFTVRTLQRADDADAMNRVYLRCGMVSAPVDVIWDNHLHRDPVDYLVAVRD
                     DGTIVGTVTGVDHRRLFSDPEDGSSLWTLAVDPTSSLPGVGAALTRALAAIYRDRGRA
                     YMDLSVAHDNAAAIALYEKLGFARVPVMAVKRKNAINEPLFTHPPETVEDLNPYARII
                     ADEAMRRGIWVEVLDAEAGEMRLSHGGRGVVTRESLSEYTSAVAMARCDDKRLTRRLV
                     AEAGIAVAKGRLATFDEQDYAFLDEVGDVVVKPTRGEQGKGITVGVDNPDELDAALAR
                     AREQHPDVLIEQRASGDDLRLVVIDHKVVAAALRRPAEVIGTGHHTVRELIDAQSRRR
                     AAATGGESRIPVDDVTEATVREAGWSFDDVLPEGRRLRVRRTANLHQGGTIHDVTAEV
                     NPDLCSVAVTAADAIGIPVTGIDLLVPDVTGTEYVFIEANERPGLANHEPQPTAQAFV
                     DFLFPQSPGLPQAWTPDQPPS"
     gene            complement(13384..15183)
                     /locus_tag="VFC01_33235"
     CDS             complement(13384..15183)
                     /locus_tag="VFC01_33235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HZN84520.1"
                     /translation="MCGATGEVRLDGQTPDITAVSAMAQAMAPRGPDAAGVWSQGRVA
                     LGHRRLKIIDLSEAGAQPMVDSELGLAVCWNGCIYNYKQLRRELSEHGYRFFSHSDTE
                     VLLKAYHHWGDRFVDRLFGMFAFAIVERDSGRVLLGRDRLGIKPLYLTEDAHRIRFAS
                     SLPALLAGGDVDTRIDAVALHHYMTFHSVVPPPRTILRGVTKVPPASLVAIEPDGRRT
                     TTTYWEPDFSRRDDRADWSEQDWEEAVLAALRVAVDRRLVADVPVGCLLSGGVDSSLI
                     VGLLAEAGQHGLATFSIGFESVNGVKGDEFRYSDIIAERFETDHHQIRIGTDRMLPAL
                     DGAIGAMSEPMVSHDCVAFYLLSQEVAKHVKVVQSGQGADEVFAGYHWYPPMADATSL
                     EGSVASYRKAFFDRDSDGVAGLVTPAYLADGDPGEQFVTEHFAHAGAATGVDRALRLD
                     TTVMLVDDPVKRVDNMTMAWGLEGRVPFLDHELVELAATCPPELKTAHEGKGVLKQAA
                     RKVIPADVIDRPKGYFPVPALTHLEGPYLDLVRDALYAPAAKERGLFRPEAVDRLLAD
                     PNGRLTPLRGNELWQIALLELWLQRHGITGAAA"
     gene            complement(15237..16808)
                     /locus_tag="VFC01_33240"
     CDS             complement(15237..16808)
                     /locus_tag="VFC01_33240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN84521.1"
                     /translation="MPEGHEHLTEDERHLAKLGYVQELQRSWSGFSNFAISFSIISIL
                     AGCFTSFGLGWNNGGPMAIAWGWPLVSIFILIIGLCMSELVSAFPTSGGIYWWASKLG
                     GVKAGFYTGWLNLIGLIAILASVAYGCATFLDLTLGTFSETWLNGYSLTRTFILFVVI
                     LAISALINIFSSHLLAIINNISVWWHVAGAAAVIVILWAIPEQHASFGEVFAKTINNS
                     GMFGGSTSGWGFLLFVLPIAAILTQYTITGYDASAHLSEETMSAASGAAKGIWQSIFY
                     SAIGGWILLLSFLFAVQDSDAVSAGGGAVVTIFNQAMSSKWVALILFISTAGQFFCTT
                     ACQTSASRMLFAFSRDRAVPGHQLWSKISAKRVPANGVMVTAVLAAIITLPALVEVDI
                     NGAPVPVAFFAVVSIGVVGLYLCFAVPIYYRWKAGDSFEQGSWNLGNHWKWMAPIAII
                     EILITSAVAMFPTSSGGVPWGDAFEWKFVNYTPLLVGGVLVLLWIYWHASVKKWFTGP
                     IKQVDETGEELEGVS"
     gene            17055..18629
                     /locus_tag="VFC01_33245"
     CDS             17055..18629
                     /locus_tag="VFC01_33245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZN84522.1"
                     /translation="MADNKRAVGSVTYRDAEHGYFEKRKLRRHAAFWSLWALGVGAVI
                     SGDFYGWNLGLDAGGFGGLLIAGIVITIMYYGLCYSIAEMSPALPHTGGAYSFSRSAM
                     GPWGGFITGLAENMEYVITPAVVVGAMGFLSHDIVFDLTGADGWWNSPVLWWAIYYIV
                     FVGINILGIEITMRFTVVITAAALGILGFFFLAVLFTGSFDAGLLTNIPPDEGGSSFL
                     PKGIAGIFPALPFAIWFYLAIEELPLAAEESHDPKRDIPKATIWGLNTLVLFAVLTLF
                     LNTGVGGGAAAIGTSATPLFDGFKAVFGEGTAASLLALVGLIGLIASFFTIIYAYGRN
                     TYSLSRAGYFPQSLSITHPTRNTPYVALIAGAVVGYILSLVIYFLGQSENALAGKIVG
                     ALLYMAVFGAVISYFMQCLAFIMLRRKLPNIARPYRSPVGVWGAAIAGAIALVALVSL
                     FLNPDYRPGVVGTLVYFVIGVIYFAVAGRHKLVLSPEEEFALSHGQYGVNLEQEGYGA
                     MSATEIATDESPTRSP"
     gene            18645..20162
                     /locus_tag="VFC01_33250"
     CDS             18645..20162
                     /locus_tag="VFC01_33250"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HZN84523.1"
                     /translation="MSLVLGVDVGSQSIKVVLVDAGGGVVATGSAALSMTHQHDGWAE
                     QDPRMYCTALRDAVRAATSDVDPARIIAMGLGSQVDGVVACDADGEPLRPAIIWLDKR
                     ATEQCDRLVAAVGEDTLAERTGLVADASHSAPKMMWIRDHEPLVWQRTATLAPVGSYL
                     LHRLTGSHAQDAANASSTMVYDVAHGDFDESLCTAAELDPSMLAPVRRSTDVVGTLLP
                     DMASEFGLPASCAVVVGTGDEHAASVGAGAIEAGVVVDVTGTAEPVTTVSATPVRDPA
                     GLVETHGHAVPGAWLIENPGFVSGGSTLWLAGLLGVPQSEVFPLACKAPPASEGVLFL
                     PALSGATAPRWNDRMRGGLLGLAMNHTPAHVARAVLEGCAFALRDIVERLDALGLGRG
                     EVRIVGGGARDNLWAAIKASVLGRPVRRVLTEEATAVGAAMVAGVGAGLFADFAAAMV
                     AVRLAPVAIDPDPEATKTYADAYRRYLAAFDAVESALAAPAVSSARPTEDSVRST"
     gene            20159..21520
                     /locus_tag="VFC01_33255"
     CDS             20159..21520
                     /locus_tag="VFC01_33255"
                     /inference="COORDINATES: protein motif:HMM:NF025065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZN84524.1"
                     /translation="MITVPDPAALLASLRADGQPGLPDIDVRIGASVSDSAIADSLAR
                     FIGRDPIILSDGGPYRTAAGAVVDRLAGHLGCRVIQLGTGTVRADEETVEQSVAEVGE
                     HRLLVSVGSGTLTDIAKVTAQRTGNHHVAIQTACSINGFIADRSVLVIAGAKRTVHSR
                     WPDVLVADTDILSAAPWQLNLAGVGDLSTVPNAVAEWRLAARLGHGPPYAPAVVDDVL
                     AAHPVLPALARAARDAEPAGIADLARLLAVSGLSMGIVGSTAPASGTEHAVSHLMEMA
                     RSRQGRPAAAHGMQVTVATRLAVRVWQLVDATIRSADAKVRVADETTSRDAVARAFAE
                     FDPQTAAECWTAYSAKRNWLLANQSAVEALVSDWDAFVHSLALASPEQFDEISHASGL
                     PTLAQDLGAGYDDDLLFWALRNSHLLRERISIVDLADLLGVWSDDTARSIVADAAKAG
                     AKE"
     gene            21531..22223
                     /locus_tag="VFC01_33260"
     CDS             21531..22223
                     /locus_tag="VFC01_33260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HZN84525.1"
                     /translation="MSEFVFMLTHGDRTVETAADIVPQLADTGLRYIGFKDVGADASR
                     QRELTSLAHDAGFEVMLEVVSTSKEDELSSLHAAAAAGVDWVLGGTHASDGAGILPDG
                     VRYCPFPGKVVDHPSVLLGTIDEIASDAERLTALDHVSGLDLLAYRHPTADHESLIRA
                     VVARSSGPVIVAGSIASIDRVRAVSEAGAWGYTIGSAIFDGLLPGAPSVPDQVRRVLE
                     ATAAEAPRETRT"
     gene            22234..22983
                     /locus_tag="VFC01_33265"
     CDS             22234..22983
                     /locus_tag="VFC01_33265"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN84526.1"
                     /translation="MSGRTLLADRLRDELLEEITSSQLQPGTKLPTEGQLAKRFNVSR
                     ATVREAVRGLVEAGYVTRRRGSGSYVAERRRMPHGLDSTLSYLAMIETAGAHAGMRIL
                     QAAFEPCRQEDAPLQLGPRDTVLAVERVRTADDQPVIYSLDRIPARLLRTDLDLQHLD
                     PSLFALLSSSGHAADHATATLRAVASTSQTAKVLGVRRGRPLLYIEEVDYGRDGTPVM
                     LSREWHVSEAFDVRINRKASTLQALPASTTA"
     gene            23093..24451
                     /locus_tag="VFC01_33270"
     CDS             23093..24451
                     /locus_tag="VFC01_33270"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZN84527.1"
                     /translation="MTGMLSQSELEALVSAGDIDTVIVAFCDMQGRLTGKRVSARLFV
                     EDVAAHGAECCNYLLAVDVDMNTVDGYAISSWETGYGDMVMTPDFSTLRLIPWLPGTA
                     MVMADLSWTDGNPVTQAPRSILNRQIDRLAESGLVPYVGTELEFMVFDTGFREAWAAG
                     YRGLAPATDYNVDYAMHASTRMEPLLRDIRLGMDGAGMYCEGVKGECNLGQQEIAFRY
                     DHARVTCDNHTIYKNGAKEIADKYGKSLTFMAKFDEREGNSCHIHISLRGDDGSAVFA
                     DSDDELGMSPMFRSFIAGQLATMRELTLFYAPNINSYKRFVEGSFAPTAIAWGLDNRT
                     CALRVVGQGHAMRMENRAPGGDVNQYLAVSALIAGGLYGIDRELELPEALEGNAYTSG
                     AERLPTTLAEAAALFGKSEVAREAFGDDVVEHYLNNARVELAAFNSAVTDWERVRGFE
                     RL"
     gene            24432..25178
                     /locus_tag="VFC01_33275"
     CDS             24432..25178
                     /locus_tag="VFC01_33275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZN84528.1"
                     /translation="MALSGSRPVLGLTTYLQQAQTGPWDVRASFLPAIYIEGVNLAGG
                     VAALLPPQPVDAEIASRILDGLDGLIITGGRDVVPEGYGQQPHPATDEAVADNRARDA
                     LEYALVDGAIRRGMPLLGICRGAQVINVALGGTLHQHLPDVLGHTRHQQGNAVFSTSA
                     VRTVPGTRLAALIGESSDAQCYHHQAIDRLGKGLIVSAQDSDGVIEAVEIPGDNFVLA
                     VQWHPEERLDDLRLFAAVVEAAAAYAKENA"
     gene            25175..26539
                     /locus_tag="VFC01_33280"
     CDS             25175..26539
                     /locus_tag="VFC01_33280"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN84529.1"
                     /translation="MTVSELINPATEEVLRTVEQTDDAGVDDAVARAKAAQREWARRA
                     PAERAAALRAFAATVDAHVGELAELEIVNSGHPIGNAEWEAGHVRDVLQYYSATPERL
                     SGKQIPVAGGLDITFNEPLGVVGVITPWNFPMTIAAWGFAPALAAGNAVLVKPAEWTP
                     LTTMRLGELAVDAGLPADLFQVLPGKGSVVGERFVSHPDVRKIVFTGSTEVGTRVMAG
                     AAAQVKRVTLELGGKSANIVFDDCDLEKAAATAPYGVFDNAGQDCCARSRILVQRNVY
                     DRFMELLEPAVKGVVVGDPRNKDTEMGPLVSKPHWNTVASYVPDDAPVAFRGDAPSGP
                     GYWFPPTVLTPARTDRTVTEEIFGPVVTVLPFDDEADAIALANDTPYGLSGSIWTDNL
                     SRALRVSRAVESGNLSVNSHSSVRYNTPFGGFKQSGLGRELGPDAPLSFTETKNVFYA
                     IGDA"
     gene            26536..>27285
                     /locus_tag="VFC01_33285"
     CDS             26536..>27285
                     /locus_tag="VFC01_33285"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HZN84530.1"
                     /translation="MMDLTQRLAGKVAVITGGAGGIGLATGRRMHAEGATIVVGDIDP
                     TAGKAAADELDGLFVPVDVSDEAAVDNLFDTAADTYGSVDIAFNNAGISPPDDDVIET
                     TELPAWQKVQDINLKSVYLSCRAALRHMVPVKKGSIINTASFVAVMGSATSQISYTAS
                     KGGVLAMSRELGIQYARSGIRVNALCPGPVNTPLLQELFAKDPERAARRLVHVPMGRF
                     AEPEELAAAVAFLASDDSSFITGSTFLVDGGI"
BASE COUNT         4590 a         8901 c         9187 g         4607 t
ORIGIN      
        1 cccaggtacg cgagcttgcg gcgttgacac ggtcgtcgcc ggtgctcagc gcgtggacgc
       61 tgcagatcta cctgcgctac gaggccgcga tcgcggatct ggtcgccccg cgactcggca
      121 gcgccgctcc cgacgacccg cggccgcggc tgctcggcgc gctcgcgatg gcatcgatac
      181 ggatcgcgct cgacgactgg ctggcccacg gcggctcgct accggcgcgg gtgcgcacgg
      241 cactgtcgtc cgtgcgcatc gagtgatcag cgccgaaaca gctgcgagcc gatccgaacc
      301 agccggtccc acccggccgg gcgcgcgacc agcttgccga gcaacggtgc ggagtcgaag
      361 tacgaaaccc gctcggcgat aaggccattg gtgaacgtga aacgatcgac cgcatcccag
      421 gcgatcggct tgccgccata cgttccgctc agcgtgaatt caatgaacac ctcgtgcttg
      481 ccgtgcccaa cgcggtgcac gtcggcgtgc aggtcgggaa ccaaggcgaa cagtcgctga
      541 aacgactcgc ggcaggcatg cttgccgcgc agggtgccca tcatcggctg gatcagcacg
      601 atgtcgtcgc tccacaacgc ttcgtgcttg gccaggtccg gagtgcgcca ggtctcggcg
      661 aaacgatcgg cgaactccgt cgcgaacttc tcgtcaacct cgtcgatggt catggcacat
      721 gaatgtagcg acgcggggga gtgctgcggt ccgatcagtg cggttgaggt gtgcgacgcg
      781 tcctaatctg tggtctggcc gtccgtttgt tcgtcagcgc ggatccagca ccgcgaagct
      841 caacgtcgca cgcgcagcca gccgatcgcg gccgacgtcg gtgacgtcga cggccgtcac
      901 gacgagccgc ttgccggcgc ggacgatcgt cgcctcggcc cgtgccggcc ccacgacgac
      961 gggcgcgagg aagtgcacgt tcatgtccgc ggtggtgaca ctctgatcgg ggccgacatg
     1021 gtgttccgcc aaccggcctg cggcgatgtc gatcagcgtc gcaaccaagc cgccctgcag
     1081 ggctccgcgg atattcgtca gatcgggccg attgtccatc tcgatgatca ggcgttcgtc
     1141 ggtatcgacc acatcgcgca tgcccatctg cccgagcaga tggccttcgg tggttctcat
     1201 cggcctcgat tcgctgagca tggctaacaa ggtagcatca ttctcgaaat cagagattgc
     1261 cattctcata gctatcctag gcgtcatgcc tgttcgcacc gcccttcgtc ccggcgtgct
     1321 gtcaccgacg ctgccggtgc cccgcacgat cgcacctccg gagtacgcgt ggaagccgac
     1381 cgtcaaagag ggcagcgagc cgtgggtgca gacgcccgag gtgatcgcga agatgcgtat
     1441 ctccggccgc atcgccgcgg gcgccctggc cgaggcgggc aaggccgtcg ctcccggcgt
     1501 gacgaccgac gagctggacc gcatcgccca cgcgtacatg atcgaccacg gcgcgtaccc
     1561 ctcgacgctt ggctacaagg gcttcccgaa gtcctgctgc acgtcgctca acgagatcat
     1621 ctgccacggc atacccgact cgaccgtggt cgaggacggc gacatcgtca acatcgacgt
     1681 cactgcctac atcgacggtg tgcacggcga caccaacgcc accttcctgg ccggcgatgt
     1741 gtccgaggag catcgcctgc tggtcgagcg cacgcaggaa gcgaccatgc gcgcgatcaa
     1801 ggccgtcaaa cccggcaggc agctgtcggt cgtgggccgc gtcatcgagt cgtatgcaaa
     1861 ccgcttcggc tacaacgtcg ttcgcgactt caccggacat ggcatcggca ccaccttcca
     1921 caatgggcta gtggtgctgc actacgacca gcccgccgtg gagacggtgc tcgagccggg
     1981 tatgacgttc accatcgagc cgatgatcaa cctcggcgcg ctggattacg agatctggga
     2041 cgacggctgg accgtcgcca ccaaggacaa gaagtggacc gcgcagttcg agcacacgct
     2101 ggtggtcacc gacgacggcg cggagattct gacccagctg tgagacttcc agcgcgagca
     2161 gacgcaaaac tgccctattt ggcgcgaaaa aggacagttt tacgtctgct cggcatagaa
     2221 aagtgacccc ctcgggcgcg ctgttggttg ctggcaccac gtcggatgcc ggcaagtcga
     2281 tggtcgtcgc aggactgtgc cggctgttgg cgcgcaaggg cattcgtgtc gcgccgttca
     2341 aggcgcagaa catgtcgaac aactcggcgg tcaccgtcga aggcggagag atcggccggg
     2401 cacaggccat gcaggcccgc gcggcggggc tggcacccag cgtccggttc aacccgatcc
     2461 tgctcaagcc cggcagcgac cgcacctcac agctcgtgct gcgcggccac gtcgtcgaca
     2521 cagtgagtgc acacgattac ttcacccacc gtgaccggct cgctgccgtc gtggctgacg
     2581 aattgcgcca gctgcgaagc gaattcgacg ccgtgatctg tgaaggtgct ggctcgcccg
     2641 ccgagatcaa cctgcgcgca accgatctgg cgaacatggg gctggcccgt gccgcggagc
     2701 tgcccgtcat ccttgtcggc gacatcgacc gcggcgggct gctggcccac ctgtatggga
     2761 ccgtcgcggt cctcgaaccc gacgaccagc gactcatttc cggattcgtg gtcaacaagt
     2821 tccgcggcga cccctcactg ctggcacccg gactggagca gctggaagcg ctgaccggcc
     2881 ggccgacgta cggcgtgatt ccctacaccg acggactgtg gctcgacacc gaggattcgg
     2941 tgtcggtgct cgcacaccgc gtcgtcggtg acccgcagcc gccccgcggc gcggactggc
     3001 tgcgcgtggc cgcgatccgg ctgccgcgca tctcgaactc gaccgacatc gaagcgctgg
     3061 cgtgcgagcc gggcgtggtg gtgcgctggg tgaccgatcc cgcccacctg gctgacgccg
     3121 atgtggtcgt gattcccggc agcaagtcca cggtcgccga cctgacctgg cttggcgaaa
     3181 aaggcttggc cgacggcatc accgtgcatg cccggcaaaa caagccggtc ctgggcatct
     3241 gcggcggctt ccagatgctc tgcaagacca tcgacgaccc agtggaatcc aaagccggcg
     3301 tcgtcgaagg cctcggcctg ctcgacgccg acatcgtgtt cgcgccggat aagacgctgc
     3361 gccaccacga cagcccgcta tgcggctacg agattcacca cggccaagtc gcgcgccacg
     3421 gcgacggcga ctggcacggt gtcggaatcc gcagcggccg cgtctatggc acgcactggc
     3481 atggactgtt ggacaacaac gacctccgcc gccagtggct gacagaggtc ggaacgcccg
     3541 ggttcgtcgt cgccgacgac gtcgacgtgg cggcgcgccg cgacgctcag ctggacctga
     3601 tggccgatct gctcaccgcg catcttgatg tcgatgccgt cgtcgcggtg ttggaaagcg
     3661 gtccgccaca acggccaatg atcgtcacgg ggctgcgcca ctgacgacga gctgatgtcc
     3721 ggcgcgtacc ttctcgccag gcattgcgca cgaggtgcgc gtcaccagcg aaacgggcag
     3781 cacggttcgc ctgcgcagtc gccgagtggg ctggtccaac cgcgtgaaga gacggtcggc
     3841 ggcgaaacgt cccatctcgt caccgtcctg gtggatcacc gtaacggcgg gcttcaatgc
     3901 agccgccgtc gggaagtcgc caaaacccac tagtccaata tccttgcggc gcaacgcctg
     3961 cagcgccgtc acgagggcga tcgtgacgcg cgcgttcgag gagaacacag cgctcagcgg
     4021 gtcggatgct gcgagcatgc ggcgcagctc cgctgtgagc gttcccgcgt cgatagcgcc
     4081 cacgtgtgtg agatcgctgc gctgtggcag ccccgcctcg tccagtgcgt tctgcaaccc
     4141 cttcagccgc agtaatccgg tgtatgtgtc gtcgcccatg aacgcgatgc tgcgatgacc
     4201 gtgcgaaatc agatgccggc ccgcctgatg tccgccaccg acgtcgtcct gcaggaccgt
     4261 atcggcggtc aaccgtccgg gttctcgatc gacgaacacc aacggtgtgc gcttctgcca
     4321 tggctgcaag tacgacatat cgcggccgac cggacagatg atcatgcccg ccacctggcg
     4381 cttcaatacc gcttcgatcg actgccgttc gtgcgacggc tcccagccga cggcggtcac
     4441 gatcaccgcg acaccccgcc ggcttgcctc gcgctcgact gcgccgatga tgctggcgaa
     4501 gaacgggtcg gccacgccgg gcaccgcaac gccgatcgcg gcgtcgcgtc cgctacggaa
     4561 ggtgacggcg agcatgttcg gcacgtactg gagttcttcg atcgcccgct cgacccgttc
     4621 gcggacgtcg gcggagacat agcggtcatt gttgaccacg cgcgagaccg tcttggcgct
     4681 cacccccgcc cggccggcaa cgtcgcgcat ggtcgccatc gagctcccct tctcggtcgt
     4741 tcagcggtca gtatgtcacc gatgtcagac cgaacggtgg agtttctctg acaccggtga
     4801 catagccgcg aatgtggcgt actgtccggc acaagcccat cgcaacgtga cccaggtaac
     4861 agacccgcca gacgaggagg ggccaacacc gtcgtgcaac ctcagctcct gcctcccaac
     4921 gtggtaccgc actggtatgc aggcggatcg gcgctggccg cctggcgcgg tctgccctcg
     4981 gtcggtgagc gctcgccgga ggagtgggtt ggcgcgacgg tggcacgctt cggtgccccg
     5041 aacctcgggc ctgcccgcct ggccgacggc acgctgcttc gtgatgcggt cagcgccgac
     5101 ccgctcggct ggctcggccg cgacgacgcg gaaccgggtg acaccggcgt gctcgtcaag
     5161 ctcttggatg ccggacaacg cctgccggtg cacgtgcatc ccacccggga gtacgccttt
     5221 caacacctgg gctgcgccta cggcaagacc gaggcgtggt ttgtcctgga agccgacgac
     5281 ggcgccgcgg tgtgggtcgg gtggcgcgag gaggtcggcc gcgaccggct catgaagctg
     5341 gtggaggcgc aggatgccga ggcgatgctg gcgctcatgc atcgtgttcc ggtgcgaccg
     5401 ggcgacggcg tgctggtgcc aggcggagcc ccgcacgcca tcggtgcggg cattctcctt
     5461 gtggaggcgc aggagcccac cgatcagtca attctgttgg agcgcaccaa caccacggca
     5521 tccgacgacg agatttttct cggcctgacc cgcgaggtcg cgctgtcggt ggtcgagacc
     5581 acagcacgga cgaacgtctc tgcgctcacg cggcacaccg ataagcacgt ggccggcctc
     5641 gtgcaggtgc tgaccgagca ggcggagccc ttcttccgca tggcattact gacagcgggt
     5701 gtcgacatgc ccgccggatt cgcggtcgcc gtggtgctgt ccggtgcagg cacactcgcg
     5761 gcggcgcaca gcgaaaccgt cgacgtcgaa cgcggccaga cactcgtcgt cccagcgtct
     5821 tccggggatt ggcacctcga cggtgacgtg cagctgcttg tctgtaggcc gggcaccacg
     5881 tggccgccaa agcccacgcc gaacatcaag caacaggaag gcgcagcatg accgccctgc
     5941 tggaggcgcg cgccgtctcg cggagcttcg gccacgtccg tgcgctggac cgcgccgact
     6001 tcgacatcag tcccggcgag gtcgtcggct tgatcggcga caacggcgca ggcaagtcga
     6061 cgctcatcaa ggccttgtcg ggcagcctcg aattggatga gggcgagatc tacttcgacg
     6121 gcaagccagt gcgtttgtca tctccacgac aggcgaacga cctcggcatc gaggtggtgt
     6181 accaggatct ggcgctggct cctcatctca atcccgttca gaacgtgttc ttgggcaggg
     6241 aaatcccggc caagggcgtt ctgggccacc ttggtttcat ggacgagaag gagatgcgtc
     6301 gccaggcggc tgcgtccttc agggcgctcg gggccacggt gcgttcactc agctcgcccg
     6361 tcgggtcgat gtccggtgga cagcgccagg gcatcgccat tgccagggcc atcgcatggg
     6421 cggacaaggt cgtcatcctc gacgagccaa cagcggcgct gggtgtcgtc cagaccaaga
     6481 acgttctcga gtcggtaaag cgggtgcgtg acaagggaat tgcggtggtg ttcatcagcc
     6541 actcgatgcc gcatgtgctc gaggtgtgcg accgcatcca ggtgctgcgg ctcggccggc
     6601 gggtcgctac gtaccggggc cgcagcacga cggtcgagac gcttgtgggt gccatgacgg
     6661 gcgccctcga tgcgaaggac ggtggggcat gagtaccgaa gacgacgacg agtgaggatc
     6721 gcggcgagga acgagcgagg accggaggga ggaggagtcg aggcgtgagc accgtggcca
     6781 accagcagga cgccgtggcg gacagggcag acgagctcga cagtgcccag tcactgctga
     6841 aacgcgttgt ggggcttcaa gcgttctgga tcctcggggt gctgatagtc atctgcttgt
     6901 tcttcaccgt cattgccggc gaccgtttcc tgtcgaccgg caacttctcg ctcatctcac
     6961 agaacgtcgc tgtctgggcg gtgctcggcg tcgggatgac gttcgtgatc atcacctcgg
     7021 gcatcgacct gtctgtcggc tcggtgctgg tgttctcgtc cgtggtcgca gccaaggtga
     7081 tggaagcgat gggcggcagt ggaattggtg ttgccgctgt cgggatcgtc gctgcgatcc
     7141 tgtccggtat ggcgtggggt gtcgtcaacg gcgcgctggt cgccatcgcg aaagtgcccg
     7201 cactgatcgt caccctcggc acgctgtcgg ttgcgctcgg actggcgcag gtcctcactg
     7261 gcggtatcga catccgctcg gtccccagcg aactgaccga cttcagcgcc tacacaaaga
     7321 tattgggcat ccccggcctg ccgttcgtcg cgctgatagt gatcgttctc ggtggcatcc
     7381 tgctgcacaa gacgagattc ggccgttaca cctatgccat cggcagcaac gaggaggctg
     7441 ctcgtcgcac gggcatcaag gtgtcgcgac atctggtgct cgtgtacggg ttggccggca
     7501 cgctcgccgg attgggcgcg ctgctctcgc tcgcccagta cggcaccacg acgatcgccg
     7561 gccagtcgtt gaccaacctc aacgtcatcg ccgcggttgt catcggcggc acatcgattt
     7621 tcggcggcta cggcacgatc ttcggcaccg tcgtcggcct gttcatcccc gccgtgctgc
     7681 agtccggctt cgtcatcatc ggcgtccaac ccttctggca gggcgtcgcg gtcggctcgg
     7741 tgctcatcgc cgccgtctac gtcgaccaga cccggcgtgc cgcggccatg cgcagtgcac
     7801 ggtctcgagg cctcttcaaa cgcaaaggat cgaaatgaaa ggaaagccag tgaaccgtaa
     7861 gcgcgtaatg ccggtcgcgg gtgctcttgg ggcagtggtg ctcacgctgt cgatggcgag
     7921 ctgcacgtcc tcgaagccgc agtcgagcgg ttcgggcgag acgtcgaacg gggtgacgcc
     7981 ggcggcggca gcgaccaccg tcaccgcgcc gccaaaagcc agcaagaact acaacatcca
     8041 gcttctgcag ggtgtgatcg gcgaccagtt ctacatcacg atgcagtgcg gcgcgcagga
     8101 agaggcggcc aagctcggcg tcacgttgaa ctctcagggc ccgcagaagt tcgatccgac
     8161 gctgcagaag ccggtcctgg actccatcgt cgccgcccgc ccggccgcgc tgctggtcgc
     8221 gcccaccgat gtccaggcca tgcagcagcc cctcgagcag gccgccgcgg cgggcatcaa
     8281 ggtggtgctc gtcgatacca cgacgaacga tccgtcatac gcggtgtcgt cgatcgccag
     8341 cgacaacgag ggcggcggca aagcggcctt cgacgccatc aagcaactgc accccgaggg
     8401 cggcaaggtc atggtcatga acgtcgatcc cggcatatcg accaccgacg cgcgcgcgaa
     8461 gggcttcgag gacgcggtca aggccgacgg caagttcacc tacgtcggcg tgcagtacag
     8521 ccacgacgat ccggcgacgg cagcccaact catcggcgcg caactgcaga aggatcccga
     8581 cctcgtcggc gtcttcgcga cgaacatctt ctccgccgag ggctcggcca ccggagtcaa
     8641 acaggcgggc aagagcggcc aggtgaagat cgtcggtttc gacgccggcc cgaaccagat
     8701 ccaggcgctg cgggacggca cggtgcaagc gctggtcgcg caggagccgg ggatcatcgg
     8761 caagttcggg gtcgacgagg cggtgacggc gctcgacggc ggtcagaaca ccaagaaagt
     8821 gcagaccggc ttcaccatca tcacgcagga aaacctcaac ggcgaaggcg gtgcggcggc
     8881 gtacaagtcg agctgctgac ctacatactg ctgatctgca aacgctgtcc gtcaaagcgg
     8941 acgccgcgca gatccgcaac ggccacagtg acaccggcag tcgcggcggt gcctacgccg
     9001 atgatggttt cgacacccgc tgcttgcgct gccgcgatgc ccgcgggcgc gtcctcgaac
     9061 accgcgcacc gttgaggaga tagtcctaag cgccgcgctg cgagcaagta ggggtcgggg
     9121 aagggttttc cgcgttcgac gtcctcgccg gtgaccagta cggcggctgg gacgagtccg
     9181 gccgacacca tacgcgcggt ggcaatggtg cggccgccgg aggtgacgac cgcccagctt
     9241 ccctgaggta tggactcgag cagttcgcgt gcacctggta tcggcaccac gtcggcggca
     9301 tggtccagct ccagctgaat caggtcagcg gcggcctcgg cgacatcgct tggcgtgctt
     9361 atcaattcag cgacgacatc ccgaattcgg cggccatgct cgatgtcgcg gtggaagtcg
     9421 aaacccggtg cccagcgttg cgcccactga ttccatgcga cggccgcggc gttgtgagag
     9481 tcgacgagga caccgtcgca gtcgagtagc agcccgtcga ctctgagatt caccgcgtcg
     9541 ctcccggtca cgaggaagca gagtatgaca ttggtatggc tgatttcgcg gggcgcggag
     9601 tgcttgtaac gggcgcgagc ggcggcatcg gggcggcgac ggtccgccgg ctcgtcagtc
     9661 agggcgcgac agtttatgca ggcggacgaa acgtcgagca gctgaccaag ctgtgcgatg
     9721 agacgggcgc tgcgccggtt ccgttcgatc tcacttctga agacgagatc cgcgccgcgg
     9781 tatccggact cgacctgtgg ggcgtggtga actgtggtgg gttcggcggg gagatagcga
     9841 cgccccaaga caccgatatc gcggtgttcg acaaggtaat cagcatcaac gcgcggggga
     9901 gcctgctggt catcaagcac gcgacaccag caatgatcac gcgcggcgtg ggtggctcga
     9961 tcgtgaacgt gtccagtcaa gcgagcctgg tggcgttgtc gggccacatt tcctacggat
    10021 cgtccaaggc ggcgctcgac aacatcaccc gggtggccgc gctggaactg ggcaggcaca
    10081 acatccgcgt caacagcgtc aatccgaccg tggtgatgac cgacatgtcc gccttttact
    10141 ggggacgccc cgatgtcggt ccgccgttcc tggagcagat gccgttgggg cggtgggcca
    10201 ccgaggacga gatcgccgcg ccgatctgtt tccttctcag cgacgacgcg tcgatgatca
    10261 ccggggtcac actgccgatc gatggcggct attcctgtcg ctaacgtccg acgggctccg
    10321 cgttcgcgtc ggtagcctga aatcatgatc aggcgagccg tggtggcgct gctcttcgtg
    10381 gcgctcaccg gctgctcgca cgcgatctcg ggcaccgcga cgtggcccgg cgccaggctg
    10441 gagacggtgg tgctgaccgc cgcggacttc ccgaccggcg tgcagtacga ccgcctcact
    10501 cgcgcacccg ggcagcccga cggcgcaggc ggtccacccg ccatgctgtc caatccggaa
    10561 ggctgttcgg acgggctcac gcgcgccatc gccgactcgg ccgagcgtgg gcccggcagc
    10621 gcggcggagt atgtcgtggg ctacgacggc gcgcggatcg tgatgacggt gttgacgtgg
    10681 ccgctggatc tcgacaggct ggccgccatc gccgaccgct gcgcccattt tcaaacgttc
    10741 ttcgacccga cggcgcccgg aattccaatg acgaccacac ggctggcgac accgcgccat
    10801 gacgcgctgg tgtatcagca gaccatgcga ctgaacaacg tcgacaacag cgtctacttc
    10861 acgttcgaga acatcggcac gatggccgtg ttcggcatcg cgttccccac accaaatccc
    10921 tccattaccg tcaaagccac gctgccgcag acgtttctgg acatcgctgc aaagcaggcc
    10981 gaccgtgcac gttaagtccc gcgaaaatgg acccgccagg cgaccatttc gctgtagttt
    11041 gaccaaatgc cttccaccat ggtgaccgtc gacggctttc ctgtgccggt cgacgtggcg
    11101 ggcccggaca agggctccgt cgtggtggtg ctcggcgcgg cgcatcacgc gacggccgcg
    11161 tatgaagcgg tatgtcagcg actgcacacc gcgtcgttgc ggacgatcgt gatcggcccc
    11221 gatccgcggc tgaccgccaa atcggtggtc ggcatcctcg atgcgctcga cgtgcggtgg
    11281 gcgctactgg tcggcgaccg catcggcggc gaactggcct gggagcttgc cgccacccga
    11341 ctggaccggt tcatcgggct ggtcgtgatc gaccgcggtc atccgcgcgt cgccgacctg
    11401 accgggcaga tccgcgacga acactgccca ccggtcgaga tgaacacgac cgcgctggtc
    11461 agcacgcccg ccgcgcgggc cgtcgccaag gccagccagc gctacgtata cggcgacttc
    11521 cggctggtcg acctgctcgg caggcgcaac gcgcaggagt cgacggcaca gctggccgcc
    11581 gagatcgtca tgcgcaccag cacctggtaa cggctgctag cgactaactg ggcggctgat
    11641 ccggtgtcca ggcctggggg aggccgggac tttgcgggaa caggaagtcg acgaacgcct
    11701 gcgcggtcgg ttgcggctcg tggttggcca gtcccggccg ctcgttggcc tcgatgaaca
    11761 catactcggt cccggtgacg tcgggaacga ggaggtcgat gcccgtcacc gggatgccga
    11821 tcgcgtcggc cgccgtcacc gccaccgagc acaggtccgg gttcacctcg gctgtgacat
    11881 cgtggatggt gccgccttga tgcaggttcg ctgtccggcg cacgcgcagc cgtcggccct
    11941 cgggcagcac gtcgtcgaac gaccagccgg cctctctgac ggtggcctcg gtgacgtcgt
    12001 cgacggggat tcgggattcc cctccggtcg ccgccgcgcg gcgccggctc tgggcgtcga
    12061 tcagctcacg cacggtgtgg tggccggtgc cgatgacttc cgcggggcgt cgtagcgcgg
    12121 cggccaccac cttgtggtcg atcacgacca agcggaggtc gtcgccggat gcgcgctgct
    12181 cgatgaggac gtcgggatgt tgctcacgcg cgcgcgccag cgccgcatcg agttcgtcag
    12241 gattgtcgac gccgacagtg atgcccttgc cctgctcacc gcgggtcggc ttgaccacga
    12301 cgtcgcccac ctcatcgagg aacgcgtaat cctgctcgtc gaaggtggcc agccgacctt
    12361 tggccacggc gattcccgct tccgcgacga ggcgtcgggt gagtcgttta tcgtcgcacc
    12421 gggccatcgc caccgcggat gtgtactccg acaaggattc tcgggtgacg acgccgcgtc
    12481 cgccgtgcga cagccgcatc tcacccgctt cggcgtcgag cacctccacc cagatgccgc
    12541 ggcgcatggc ttcgtcggcg atgatgcgcg cgtacggatt tagatcctcg actgtctccg
    12601 gcgggtgagt gaacagcggt tcgttgatcg cgttcttgcg tttgaccgcc atcaccggca
    12661 cacgcgcgaa acccagcttc tcgtacagcg cgatcgcggc ggcgttgtcg tgtgccacgg
    12721 acagatccat gtaggcgcgt ccgcggtccc ggtagatcgc ggcgagcgcg cgtgtcaacg
    12781 ccgcgccgac accgggcagg ctcgacgtcg ggtcgacggc cagcgtccac aggctggagc
    12841 cgtcctcggg atcggagaac agccgccggt gatcgacacc ggtcacggtg cccacgatgg
    12901 tgccgtcgtc gcgcaccgcg acgaggtagt cgaccgggtc gcggtgaagg tggttgtccc
    12961 agatgacgtc gacgggggcg gacaccatcc cgcagcgcag gtacacgcgg ttcatcgcgt
    13021 cggcgtcatc ggcgcgttgc agggtgcgca cggtgaaccc ggttggcgac aagtccagtt
    13081 cgtcttcgga gaaccgcaac cggtaggtgt ggctcgggtc gatgaacagt tcggcgggcg
    13141 ccagggcgac cagcacctgc ggttcgcggg cgtacatgca gatgtcgcgc ctgccttgcc
    13201 cctcctggcg cagcacctcc gcgagcttgt tcggatcggc gaaggtctga ccgaaaatca
    13261 gtcggcccca accgagttcg acgacgacgt cgtcggccat ggcgtcgacc atgtgctgcg
    13321 gcgacgcgtc atggaggcca agcgtgatgg cctcggaatg gtcctgcgct ggatctgtcg
    13381 cggtcatgcc gcggctcccg tgatgccgtg ccgctgcaac cacagctcga gcagcgcgat
    13441 ctgccacagc tcgttgccgc gcagcggcgt gagccggccg ttgggatcgg cgagcagccg
    13501 gtcgacggcc tccggtcgga acaggccgcg ctccttggcg gccggggcgt acagcgcgtc
    13561 acgcacgagg tccagatacg gaccttccag atgggtcaac gcgggcaccg ggaagtaccc
    13621 cttgggtcgg tcgatgacgt cagccgggat caccttgcgc gcggcctgct tgagcacgcc
    13681 tttgccctcg tgcgcggtct tcagctcggg cgggcaggtg gcggccagct cgaccagctc
    13741 gtggtcgagg aacggcaccc ggccttccag accccacgcc atggtcatgt tgtcgacgcg
    13801 cttgaccggg tcgtccacca gcatcacggt cgtgtcgagc cgcagcgcgc ggtcgacgcc
    13861 ggtcgcggcg cctgcgtggg cgaagtgctc ggtgacgaac tgttcgccgg gatccccgtc
    13921 ggcgaggtag gccggtgtga cgagcccggc gacaccgtcg ctgtcccggt cgaagaaggc
    13981 cttgcggtaa ctggccaccg aaccctcgag cgaggtggcg tcggccatcg ggggatacca
    14041 gtggtagccg gcgaacacct cgtcggcgcc ctgcccggac tgcacgacct tgacgtgttt
    14101 ggcgacttcc tggctgagca gatagaaggc gacgcagtcg tggctgacca tgggctcgct
    14161 catcgcgccg atcgcgccgt cgagcgcggg cagcatccgg tcggtgccga tccggatctg
    14221 atggtggtcg gtctcgaagc gctcggcgat gatgtcggag tagcggaact cgtcgccctt
    14281 gacaccgttg accgactcga agccgatgga gaacgtcgcg agaccgtgtt ggcccgcctc
    14341 cgcgagcagc ccgacgatga ggctcgaatc gacgccgccc gacagcaggc atccgacggg
    14401 cacgtcggcg accaggcggc gatccaccgc cacccgcaga gcggcgagaa ccgcttcctc
    14461 ccaatcctgt tcggaccagt cggcgcggtc gtcgcgacgg ctgaagtcag gttcccaata
    14521 tgtggtggtc gtgcgacgcc cgtcgggctc gatcgccacc agcgacgccg gcggcacttt
    14581 ggtgacgccg cgcagaatcg tccgaggcgg cgggacgacc gaatggaagg tcatgtagtg
    14641 gtgcagcgcg acggcgtcga tgcgggtgtc gacgtcgccg ccggcgagca gtgcgggcag
    14701 tgacgacgcg aaccggatcc ggtgcgcgtc ctcggtcagg tacagcggtt tgatgcccag
    14761 ccgatcacgg cccagtagca cccggccgct gtcacgttcg acgatcgcga acgcgaacat
    14821 gccgaagaga cggtcgacga accggtcacc ccagtggtgg taggccttga gcaaaacctc
    14881 ggtgtcgctg tgcgagaaga accggtagcc atgctcgctc agttcgcgcc gcagctgctt
    14941 gtagttgtag atgcaaccgt tccagcagac ggccagtccc agctcggaat cgaccatcgg
    15001 ctgggcgccc gcctcggaca gatcgatgat cttcaggcga cggtggccga gcgcgacccg
    15061 gccctgtgac cacacccccg cggcatcggg cccccggggg gccatcgcct gcgccatggc
    15121 cgagacggct gtgatgtcgg gcgtttggcc gtcgaggcgc acctcgccgg tggctccaca
    15181 cactcagccg accctacccg ctggcgggcc ttgtcaaacc tctagctgtg tagtggctag
    15241 gagacgcctt ccagttcttc gccggtttcg tcgacctgct tgatcgggcc ggtgaaccac
    15301 ttcttcaccg acgcgtgcca gtagatccac agcagtacaa gcacgccgcc caccaacagc
    15361 ggggtgtagt tgacgaactt ccactcgaag gcatcacccc acgggacacc gcccgacgac
    15421 gtcgggaaca tggcgacggc cgatgtgatg aggatctcga tgatcgcgat cggggccatc
    15481 cacttccagt ggttgccgag gttccagctg ccctgttcga acgagtcgcc ggctttccac
    15541 cggtagtaga tcggcacagc gaagcacagg tacaggccga cgacgccgat cgacaccacc
    15601 gcgaagaacg caacgggaac gggtgcgccg ttgatgtcga cctcgaccag cgcgggcagc
    15661 gtgatgatcg ccgcgagcac ggccgtgacc atcacaccgt tggccggcac ccgtttcgcg
    15721 ctgatcttgg accacagctg gtgacccggc acggcgcggt cacgactgaa cgcgaaaagc
    15781 atgcgcgacg cgctggtctg gcacgccgtg gtgcagaaga actggcccgc cgtggagatg
    15841 aacaggatga gcgccaccca cttcgaactc atcgcctggt tgaagatcgt caccaccgcg
    15901 ccaccgcctg cggacaccgc gtcggagtcc tggaccgcaa agaggaagga cagcaacagg
    15961 atccagccgc cgatggccga atagaagatc gactgccaga tgcccttggc ggccccgctg
    16021 gccgcgctca tggtctcctc ggacaggtgc gccgacgcgt catagccggt gatcgtgtac
    16081 tgcgtcagga tcgccgcgat cggcagcacg aacagcagga aaccccagcc ggacgtcgag
    16141 ccgccgaaca tgccgctgtt gttgatcgtc ttggcgaaca cctcgccgaa gctggcgtgc
    16201 tgttccggaa tcgcccacag gatcacgatc acggccgccg cgcccgccac gtgccaccac
    16261 accgagatgt tgttgatgat ggcgagcagg tgggacgaaa agatgttgat caatgctgag
    16321 atcgccaaga taacgacgaa cagaataaag gtgcgcgtca ggctgtagcc gttgagccag
    16381 gtttcactga acgtgccgag cgtcaggtcg aggaacgtcg cgcagccgta cgcgaccgac
    16441 gccaggatgg cgatcagccc gatcaggttc agccagccgg tgtagaaccc ggccttgacg
    16501 ccgccgagtt tgcttgccca ccaatagatt ccgcccgatg tcgggaacgc cgaaaccagc
    16561 tccgacatac acaggccgat gatcaggatg aagatcgaaa ccagcggcca gccccaggcg
    16621 atcgccatcg ggccgccgtt gttccagccc agcccgaacg aggtgaagca gcccgcaagg
    16681 atcgagatga tcgagaacga gatcgcgaag ttggagaagc ccgaccagga ccgctgcaac
    16741 tcctgcacgt agccgagctt ggcgagatgc cgttcgtctt cagtcaaatg ctcatggccc
    16801 tctggcacga cggttctcct tcgaggggat gggacagcgg aatttcccta cggacaatag
    16861 gccgctattg gtctggtgtc ctacctttgg ggatgacggc cgcgtaaacc ttgtccgcgt
    16921 gccgtccggt aggccaccta gctggtgttt cgggtgtttc cgaactgact ccgttccgat
    16981 attgacgtgc gcattcgccc tcattattgt ccaagttgtc tgacaacttg cgccaaatag
    17041 ccgaggaggg cgccatggcg gataacaagc gagcagtcgg atcggtcacc tatcgggacg
    17101 cagaacacgg gtatttcgag aagcgcaagc tgcgccgtca cgccgcgttc tggtcgctct
    17161 gggcccttgg tgtcggtgcg gtcatctcgg gagatttcta cggttggaac ctcggccttg
    17221 acgccggggg cttcggcggt ctgctcatcg caggcatcgt gatcacgatc atgtactacg
    17281 gcctgtgcta cagcatcgcc gagatgtcgc cggccctgcc gcacaccggc ggcgcctact
    17341 cgttctcccg gtcggcgatg ggcccgtggg gtggcttcat caccgggctg gccgagaaca
    17401 tggaatacgt catcacccct gcggtcgtcg tgggcgcgat gggattcctt tcgcacgaca
    17461 tcgttttcga cctgaccgga gccgacgggt ggtggaacag cccggtgctg tggtgggcga
    17521 tctactacat cgtcttcgtc gggatcaaca tcctcggcat cgagatcacc atgcgcttca
    17581 ctgtggtgat cacagcggcg gcgctcggca ttctcgggtt cttcttcctt gctgtgcttt
    17641 tcacggggtc gttcgacgcc ggcctgctca cgaacatccc gccggacgaa ggcggcagca
    17701 gcttcctgcc caaaggcata gccgggattt tccccgcctt accgttcgcg atctggttct
    17761 acctcgcgat cgaggaactg ccgctggccg ccgaggagtc tcacgacccg aagcgagaca
    17821 tccccaaagc gaccatctgg gggcttaaca cgctggtgct gttcgccgtg ctcaccctgt
    17881 tcctgaacac gggcgtcgga ggcggagccg cggcgatcgg cacgtcggcg accccgttgt
    17941 tcgatggctt caaggccgtg ttcggggagg gcacggcggc gtctctgctc gctttagtcg
    18001 ggctgatcgg gctgatcgcc agcttcttca cgatcatcta cgcctacggc cgcaacacat
    18061 attcgctgtc tcgcgcaggg tatttcccgc agagcctgtc gatcacacat ccgaccagaa
    18121 acacgccgta cgtcgcgctg atcgcaggcg cggtcgttgg gtacatcctc tcgctggtga
    18181 tctacttcct gggccagagc gaaaacgcac tcgcaggcaa gatcgtcggt gcgttgctgt
    18241 acatggccgt gttcggtgcg gtgatctcgt acttcatgca gtgcctggcg ttcatcatgc
    18301 tgcgccgcaa gctgccgaat atcgcacggc cgtaccgcag tccggtgggt gtctggggcg
    18361 ccgcgatcgc gggcgcgatc gcactcgtag cgctcgtctc gctcttcctg aatccggact
    18421 accggcccgg cgtcgtcgga acgctcgtgt acttcgtgat cggggtgatc tacttcgcgg
    18481 tcgcgggacg gcacaagctg gtgctgtcgc cggaagaaga gttcgcgctt tcgcatgggc
    18541 agtacggcgt gaacctggaa caggagggct acggcgcgat gtccgcgacc gagatcgcga
    18601 cagacgaatc gccgacccgc tcaccgtgat ctgaggaaaa ggccgtgtct ctggtcctgg
    18661 gagtcgatgt cggctcgcag agcatcaagg tcgtcctcgt cgacgcgggt ggcggcgtcg
    18721 tggccaccgg ttctgccgcg ctgagcatga cccaccagca cgacggctgg gcggagcagg
    18781 acccgcgtat gtactgcacc gcactgcgcg atgcggtgcg cgcggcgacc tccgacgtcg
    18841 acccggcgcg gatcatcgcg atgggtcttg gcagccaggt cgacggcgtc gtcgcatgtg
    18901 acgccgacgg tgagccgctg cgcccggcga tcatctggct ggacaaacgg gccaccgagc
    18961 agtgcgatcg gttggtcgcc gcggtcggag aggacacgtt ggccgagcga acgggtctgg
    19021 tggccgatgc ttcgcacagt gcgccgaaga tgatgtggat ccgcgaccat gaacccctgg
    19081 tgtggcagcg caccgcgacg ttggcgcccg tcgggtcgta cctgctgcac cggctgaccg
    19141 gctcgcacgc ccaggacgct gcgaacgcat cgtccaccat ggtgtacgac gtcgcccacg
    19201 gcgacttcga cgaatctctg tgcacggcag ctgaattgga tccgtcgatg ctcgctccgg
    19261 tacgccggtc gaccgacgtg gtcggcaccc tgctgcccga catggcatcg gagttcgggc
    19321 tgccggccag ttgcgccgtt gtcgtcggca ccggagacga gcacgccgcg tcggtggggg
    19381 ccggcgcgat cgaagccggc gtggtcgtcg acgtcaccgg caccgccgaa ccggtgacga
    19441 cggtgtccgc gacgcccgtg cgagacccgg ccggtctcgt cgagacgcac ggccacgcgg
    19501 tgcccggcgc atggctgatc gagaatcccg gattcgtcag cggcggaagc accctctggc
    19561 tcgccgggct gctcggggtg ccgcagtccg aggtctttcc tctggcctgc aaggcgccgc
    19621 cggcaagcga aggcgtgctg ttcctgccgg ccctgtcggg ggcgaccgcg ccgcggtgga
    19681 acgaccggat gcgtggcggc ctgctcggcc tcgcaatgaa tcacacgccg gcccacgttg
    19741 cgcgggcagt gctcgaaggt tgcgcatttg cgttgcgcga catcgtcgaa cgcctggacg
    19801 cgctcggcct cggtcgaggc gaggtgcgga tcgtcggcgg cggcgcgcgc gacaacctat
    19861 gggcggcgat caaggccagt gtgctcggca gaccagtgcg gcgcgtgctg accgaggagg
    19921 ccaccgcggt cggcgccgcc atggtcgccg gcgtgggcgc cggcctcttc gccgatttcg
    19981 ccgcggcaat ggtcgcggtc cggctcgctc cggtcgccat cgaccccgac ccggaggcga
    20041 ccaagaccta cgccgacgcc taccgccgct atctcgccgc gttcgacgcc gtcgaatccg
    20101 ctctggcggc accggccgtt tcgtcggctc ggccgaccga agattcggtt cggtcgacat
    20161 gatcaccgtt cccgaccccg cggcgctgct ggcgagcctg cgcgccgacg gtcaaccggg
    20221 actgcctgac atcgacgtac gcatcggagc gtcggtgtcg gacagcgcga tagcagacag
    20281 cctcgcaagg ttcatcggcc gagacccgat tatcctgagc gacggcggac cgtaccgcac
    20341 agcggcgggc gcagtggtag atcggctagc cgggcacctc ggctgccggg tcatccagct
    20401 cggaacgggg accgtccgcg ccgacgagga aaccgtcgaa caatcggtgg ccgaagtagg
    20461 ggagcaccgg ttgcttgtct ctgtcggatc cggaaccctc acagacatcg cgaaggtcac
    20521 cgcgcagcgc accggcaacc atcacgtcgc gatccaaacc gcctgcagca tcaacggttt
    20581 catcgcggac cgcagcgttc tcgtcatcgc aggcgcgaaa cgcactgtcc actctcggtg
    20641 gcccgatgtg ctggtcgccg acaccgacat cctcagcgcg gcgccgtggc aattgaatct
    20701 cgctggggta ggcgatcttt cgacggtccc caacgcggtc gcagaatggc ggctcgccgc
    20761 gcggctcggt cacggaccgc cgtacgcgcc cgccgtcgtc gacgatgtcc ttgccgcaca
    20821 tccggttctg cctgcacttg cgcgcgccgc gcgcgacgcc gagccagcgg gtatcgccga
    20881 tctggcccgg ctgctggcgg tcagtggact gtcgatggga atcgtcggtt cgacggcacc
    20941 ggcatcgggc accgagcatg cggtctcgca cttgatggag atggcgcgca gccgtcaggg
    21001 acggccggcc gcggcgcacg gcatgcaggt cacggtcgcg actcgactgg ccgtgcgggt
    21061 ctggcagctc gtcgatgcca cgatccggtc ggcggatgcg aaggtgcggg tggccgacga
    21121 gacaacgagc cgagacgcgg tggcacgggc gttcgccgag ttcgatccgc agacggccgc
    21181 cgagtgctgg accgcgtact cggcgaagcg aaactggctg ttggcaaacc aatccgcggt
    21241 cgaggcactg gtttcggatt gggacgcgtt cgtgcactca ttagcgctcg cgtcaccgga
    21301 acagttcgac gagatcagcc acgcgagcgg tttgccgacc ctggcacagg acctcggtgc
    21361 cggatacgac gacgacctgt tgttctgggc gctgcgcaat tcccatctgc tgcgcgagcg
    21421 gatctcgatc gtcgacctcg ccgacctcct cggcgtgtgg tcagacgaca cggcgcggtc
    21481 gatcgtggcc gacgccgcga aggccggggc gaaggaatag gagcactgcg gtgtcggaat
    21541 tcgtgttcat gctcacccac ggcgaccgga ccgtcgagac cgcggccgac attgtgccgc
    21601 aattggcgga taccggtctg cggtacatcg gattcaagga cgtcggcgca gacgcgtcga
    21661 gacagcgtga gctgacatcg ctggcccacg acgccgggtt tgaggtgatg ttggaggtgg
    21721 tctcgaccag taaggaggac gagctgtcgt cgctgcacgc ggctgctgcg gccggggtcg
    21781 actgggtgct cggcggaacg cacgcctccg acggagcggg catcctgccg gacggcgtgc
    21841 gctactgccc cttcccaggc aaagtcgtcg accatccaag cgttctgctc ggcaccatcg
    21901 acgaaatcgc aagcgacgcc gaacggctga ccgctctgga ccacgtcagc gggcttgacc
    21961 tgctggcgta tcgccacccg accgccgatc acgaatcgct gatccgtgcg gtggtggcgc
    22021 gctcgagcgg cccggtgatt gtcgcggggt caatcgcgag catcgatcgg gtgcgtgccg
    22081 tgagcgaggc cggcgcgtgg ggatacacaa tcggcagcgc catcttcgac gggctgctgc
    22141 ccggcgcgcc gtcggtgccc gaccaggtcc gtcgcgtcct cgaggcgacg gccgccgagg
    22201 cgccccgcga gacccggacg taggctcggc agagtgtccg gccgaacgtt gctcgccgac
    22261 cggttgcgcg acgagttgct ggaggaaatc accagttcgc agttgcagcc ggggaccaag
    22321 ctgccgaccg agggccaact cgccaagcgt ttcaacgtat ctcgtgccac ggtgcgtgag
    22381 gcggtgcgcg ggcttgtcga ggctggttac gtcacgcgtc gtcgcggttc gggcagttac
    22441 gtcgccgagc gtcggcgcat gccccacggt ctcgactcga cactgagcta cctcgcgatg
    22501 atcgagactg cgggcgcgca tgccgggatg cgcatcctgc aggcggcgtt cgagccgtgc
    22561 cgtcaggagg acgcgccgct tcaactcggt ccgcgcgaca cagtgttggc ggtggaacgg
    22621 gtgcggacag ccgacgacca gccggtgatc tattcgctcg accgcatccc cgcgcgcctg
    22681 ctgcgcaccg acctcgatct gcagcacctg gacccgtcac tgttcgcgct gctgagctcc
    22741 tcggggcacg cggccgatca cgcgaccgcg acactgcgcg cggtcgccag taccagccaa
    22801 accgcgaagg tgctcggggt gcggcgcggt cggcccctgc tctatatcga ggaggtcgac
    22861 tacggccgtg acggcacccc ggtcatgctg tcgcgggagt ggcatgtgtc cgaagccttc
    22921 gatgtccgga tcaaccgcaa ggccagcact ctgcaggcgc tccccgcgtc gacaacggca
    22981 taacccgata attgagttct aatggtctga tgccctacct ttaaggtggg tttttggtaa
    23041 atatgcaagg tcaggcaggc gtgaggcggt ttccaatggt tgacttctcg tcatgactgg
    23101 catgctgtcg caatccgagc tcgaagcgct ggtgtccgct ggcgacatcg acaccgtcat
    23161 cgtcgcgttc tgcgacatgc agggccgact gaccggcaag cgggtgtcgg cccggttgtt
    23221 cgtcgaggac gtcgccgcgc acggcgcgga gtgttgtaac tatctgctcg ccgtcgacgt
    23281 ggacatgaac acggtcgacg gctacgcgat ctccagctgg gaaaccggtt acggcgacat
    23341 ggtgatgacg cccgatttct ccacgctgcg gctgatcccg tggttgccgg gcaccgcgat
    23401 ggtgatggcc gacctgtcct ggaccgacgg caatcccgtg acgcaggcgc cgcgcagcat
    23461 cctgaaccgc cagatcgaca gactcgccga gagcgggctg gtgccctacg tcggcaccga
    23521 gttggagttc atggttttcg acaccggctt tcgcgaagcg tgggcggccg ggtaccgcgg
    23581 cctggcgcct gccaccgact acaacgtcga ctacgcgatg cacgcttcga cccggatgga
    23641 gccgctgctg cgcgacatcc ggctcggaat ggacggcgcc ggcatgtatt gcgagggcgt
    23701 caagggtgaa tgcaatctcg gtcagcagga gatcgcgttc cgctacgacc acgcgcgggt
    23761 cacctgcgac aaccacacca tctacaagaa cggtgccaag gagatcgccg acaagtacgg
    23821 caagagcctg acgttcatgg cgaagttcga cgagcgtgaa ggcaacagct gtcacatcca
    23881 catctcgttg cgtggtgacg acggcagtgc ggtgttcgcc gactccgacg acgagctcgg
    23941 catgtccccg atgttccgca gcttcattgc cggccagctg gcgacgatgc gcgagctgac
    24001 gttgttctat gcgccgaaca tcaactcgta caagcgattt gtggagggca gcttcgcgcc
    24061 cactgcgatc gcgtggggcc tcgacaaccg gacctgcgca ctgcgtgtcg tcggacaggg
    24121 acacgccatg cggatggaga accgggcgcc cggcggcgac gtgaaccagt accttgccgt
    24181 gtcggcgctg atcgccggcg ggctgtacgg catcgaccgg gagctggagc tgccggaagc
    24241 gttggagggc aacgcttata ccagcggtgc ggaacggctg ccgaccacgt tggcggaggc
    24301 ggcggcgttg ttcggcaagt ccgaggtggc gcgggaggcg ttcggcgacg acgtcgtcga
    24361 gcactacctc aacaacgcac gcgtggaact ggccgcgttc aattcggcgg taacagattg
    24421 ggaaagggtg cgtggctttg agcggctcta gaccggtcct gggtctgacg acctatctgc
    24481 agcaggcgca gacggggccg tgggatgtgc gggccagctt tctgcccgcc atctacatcg
    24541 aaggggtgaa cctggcgggc ggcgtcgctg ccttgcttcc gccgcaacct gtggacgccg
    24601 aaatcgcctc gcgcattctg gatgggctgg acggcctgat catcaccggt gggcgcgatg
    24661 tcgtcccgga aggctatggg cagcaaccgc atccggccac cgacgaggcg gttgccgaca
    24721 accgtgcgcg ggacgcgttg gagtacgcgt tggtcgacgg tgcgatccgg cgcggcatgc
    24781 cgctgcttgg catctgccgt ggggcgcagg tgatcaacgt cgcgctcggc ggcacactgc
    24841 atcagcatct gcccgatgtg ctcggccaca ctcgccacca gcagggcaat gcggtgtttt
    24901 ccacgtcggc ggtgcgcacc gtgcccggta cgcggctggc cgcgctgatc ggcgaatcgt
    24961 cggatgcgca gtgctatcac caccaggcca tcgaccggct cggcaagggg ctgatcgtaa
    25021 gcgcccagga ctccgacggt gtcatcgagg ccgtcgaaat ccccggtgac aacttcgttt
    25081 tggctgtgca gtggcatccc gaagaacgac tggacgacct gcgactgttc gccgcggtgg
    25141 tagaagcggc ggcagcgtat gccaaggaga atgcgtgact gtttccgaac tgatcaaccc
    25201 ggcaaccgaa gaggtgctgc gcactgtcga gcagaccgac gatgctgggg tcgacgacgc
    25261 cgtcgcacgc gccaaggccg cacaacggga gtgggcgcgg cgggcgcccg ccgagcgggc
    25321 ggcggcgttg cgtgccttcg ctgcgacggt cgacgcgcat gtgggcgagc tcgcggagct
    25381 ggagatcgtc aactccgggc atccgatcgg caacgccgaa tgggaggccg gtcacgtccg
    25441 cgacgtgctg cagtactact cggccacccc ggagcgattg tccggcaagc agattccggt
    25501 ggcgggcggg ctggacatca cgttcaacga accgctcggc gtcgtcggcg tgatcacgcc
    25561 gtggaacttc ccgatgacga tcgctgcctg gggcttcgcg cctgcactgg cggccgggaa
    25621 cgcggtcctg gtcaaaccgg cggagtggac gccgttgacc acgatgcggc tcggcgaact
    25681 ggcggtcgat gcggggctgc ccgccgacct gttccaggtg ttgcccggca aggggtcggt
    25741 ggtgggggag cgcttcgtgt cccatcccga tgtccgcaag atcgtgttca ccggctccac
    25801 cgaggtcggc acgcgcgtga tggccggcgc ggccgcacag gtcaagcggg tcaccctgga
    25861 gttgggtggc aagagcgcca acatcgtgtt cgacgactgc gacctggaga aggcggcagc
    25921 gacggcgccg tacggtgtgt tcgacaacgc cggccaggac tgctgcgcac gcagccgaat
    25981 cctggtgcag cgcaatgtct atgaccggtt catggagctg ctcgagcccg cggtcaaggg
    26041 cgtcgtggtc ggtgatccgc gcaacaagga caccgagatg ggtccgctgg tgtcgaagcc
    26101 gcattggaac accgtggcgt cgtacgtgcc cgacgatgcg cccgtcgcgt tccgtggcga
    26161 cgcgccgtcg ggtccgggct actggttccc gccgaccgtg ctgacgccgg cgcgtacgga
    26221 tcgcaccgtc accgaggaga tcttcggtcc ggtggtgacc gtgctgccgt tcgacgacga
    26281 agccgacgcc atcgcgctgg cgaatgacac gccctacggg ctgtccggtt ccatctggac
    26341 cgacaacctg tcccgcgcgc tgcgggtgtc gcgggccgtg gaatccggga atctgtcggt
    26401 caattcgcac tcgtcggtgc gctataacac gccgttcggc gggttcaagc agtcgggact
    26461 gggccgagag ctcggcccag acgcgccgtt gtcctttacc gaaaccaaga acgtcttcta
    26521 cgcgatcgga gatgcctgat ggatctgacc caacgactgg ccggcaaggt cgcggtgatc
    26581 accggtggcg ccggagggat cggactggca acgggtcggc gcatgcacgc cgagggcgcc
    26641 acgatcgtgg tcggcgacat cgatccgacc gcgggcaagg cggcggccga cgaactcgac
    26701 ggattgtttg tgcccgttga cgtctcggat gaagcagccg tcgacaacct gttcgatact
    26761 gccgccgaca cgtacgggtc cgtcgacatc gcgttcaaca acgctggcat ctcaccgccc
    26821 gacgacgacg tcatcgagac caccgagctg cccgcgtggc agaaggtgca ggacatcaac
    26881 ctcaagtcgg tgtacctgtc ctgccgtgcc gcgctgcggc acatggtgcc cgtcaagaag
    26941 ggttccatca tcaacaccgc ctcattcgtc gcggtgatgg gctcggcgac ctcccagatc
    27001 tcctacacgg cctcaaaggg cggggtgctg gcgatgtcgc gtgaactcgg gatccaatac
    27061 gcgcgcagcg ggattcgtgt caatgcgctg tgtcccggtc cggtgaatac accgctgcta
    27121 caggaactgt tcgccaagga tccggagcgg gcggcgcgca ggctggtgca cgtgccgatg
    27181 ggccggttcg ccgaaccgga ggagctggcc gctgcggtgg cgttcctggc gagtgacgat
    27241 tcttcgttca tcaccggctc gacgttcctg gtggacggcg ggatc
//
LOCUS       DASRJA010000735         9343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3771048,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000735 DASRJA010000000
VERSION     DASRJA010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3771048"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VFC01_33290"
     CDS             <1..175
                     /locus_tag="VFC01_33290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN84531.1"
                     /translation="HPRFTRHFTTQLPKDTEITHLDGVGHIPMYEAPRRVADLIVGFV
                     DRYATPPPRKAIG"
     gene            complement(172..1395)
                     /locus_tag="VFC01_33295"
     CDS             complement(172..1395)
                     /locus_tag="VFC01_33295"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZN84532.1"
                     /translation="MAKVKVEGTVVELDGDEMTRIIWKMIKEQLILPHLDVNLDYYDL
                     GIEHRDATDDQVTIDAAEAIKRHGVGVKCATITPDEARVKEFNLKKMWLSPNGTIRNI
                     LGGTIFRAPIVIKNVPRLVPGWTKPIIIGRHAFGDQYRADNFKVDKPGTFTVMFTPDD
                     GSEPIVHDVIEIPEDGGVMMGMYNFKQSIQDFARSTFNYGLQQKYPVYLSTKNTILKG
                     YDGMFKDEFQRIFDEEYKEQFDANGLTYEHRLIDDMVASCLKWEGGYVWACKNYDGDV
                     QSDLVAQGYGSLGLMTSVLMSPDGKTVEAEAAHGTVTRHYRQHQQGKPTSTNPIASIF
                     AWTRGLEHRGKLDNTPDVIKFANALEDSVVGTVEQGQMTKDLALLIGEDQPWQTSEEF
                     LDTIAGNFKKALDAA"
     gene            complement(1404..3641)
                     /locus_tag="VFC01_33300"
     CDS             complement(1404..3641)
                     /locus_tag="VFC01_33300"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZN84533.1"
                     /translation="MSAEQPTIIYTLTDEAPLLATYAFLPIIRTFTEPAGINVETSDI
                     SVAARILAEFSDYLTEEQRVPDNLGQLGELTQDPETNIIKLPNISASVPQLAAAIKEL
                     QEKGYAVPDYPAQPKNDEEKELKERYGKILGSAVNPVLREGNSDRRAPKAVKEYARRH
                     PHSMGEFSQASRTHVATMKGGDFYHGEKSMTLDKARKVRMELKTKSGETIVLKPETAL
                     DEGDVIESMFMSKKALVAFYEAEIEDAYKTGVMFSLHVKATMMKVSHPIVFGHCVKVF
                     YKDAFEKHQEVLDELGVNVNNGMVDLYNKIEKLPSFQREQIIQDIHDVHEHRPELAMV
                     DSAKGITNFHSPSDVIVDASMPAMIRLGGKMYGADGRTKDTKAVNPESTFSRIYQEMI
                     NFCKTHGQFDPTTMGTVPNVGLMAQKAEEYGSHDKTFEIPEDGVADIVDIDTGEVLLS
                     QNVEEGDIWRMPVTKDAAIRDWVKLAVTRARASGMTVLFWLDTERPHEVELRKKVKAY
                     LEDHDTEGLHIQIMPQVWAMRYTLERLIRGQDTIAATGNILRDYLTDLFPILELGTSA
                     KMLSIVPLMAGGGMYETGAGGSAPKHVHQLVEENHLRWDSLGEFLALGACFEDLGDKT
                     GNKRAALLGTTLDAAIGKLLENDKGPSRKTGELDNRGSQFYLALYWAQELAAQSEDQE
                     LAEHFAKLAKTLAEKEEAIVSELAEVQGDSVDIGGYYYPDREKTEAVMRPSKTLNETL
                     AAAHS"
     regulatory      3903..4021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            4063..5409
                     /locus_tag="VFC01_33305"
     CDS             4063..5409
                     /locus_tag="VFC01_33305"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HZN84534.1"
                     /translation="MSTPEDPTAHWSFETKQVHAGQTPDVATNARALPIYQTTSYTFN
                     STEHAAALFGLAETGNIYTRIMNPTQDVVEQRIAALEGGVAALFLASGQAAEHFAILN
                     LANAGDHIVSSPRLYGGTYNLFHYTLPKIGIEVSFVENPDDLESWRAAVKPNTKAFFG
                     ETISNPQNDILDIPNIAAVAHDAAVPLIVDNTVATPYLIQPLAHGADIVVHSATKYLG
                     GHGAAIAGVIVDGGTFDWTNGKFPGFTTPDPSYHGVVFAELGPPAFALKARVQLLRDL
                     GSAAAPFNAFLVAQGLETLSLRVERHVANAQKVAEFLAGHPDVISVNYAGLPTSPWYE
                     LGKKLAPKGTGAVLAFELAGGIDAGKAFVNALTLHSHVANIGDVRSLVIHPASTTHAQ
                     LTPEEQLATGVTPGLVRLAVGIEGIDDILADLEQGFAAAKPFSASTAADPNTAAAF"
     gene            5409..6530
                     /locus_tag="VFC01_33310"
     CDS             5409..6530
                     /locus_tag="VFC01_33310"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZN84535.1"
                     /translation="MTVIDVSTATLPAEGEVGYVDIGSLTLENGAVIDDVTIAVQRWG
                     ELSPNRDNVVVVLHALTGDSHVTGPTGPGDETEGWWEGVAGPGAPIDTDRWCAIATNV
                     LGGFRGSTGPSSSAPDGKPWGSRFPLVSVRDQVEADIAALAALGITEVAAVVGGSMGG
                     ARALEWIVSHPDAVRSALVLAVGARATADQIGTQSTQVAAIKSDPDWQNGDFHGTGRS
                     PDLGMEIARRFAHLTYRGEHELDNRFGNDPQGDEDPATGGRYSVQSYLEYQGRKLLKR
                     FDAGTYVALTEALSSHDVGRGRGGVENALRGCPVPTVVGGITSDRLYPLRLQEELAEL
                     LPGCTELNVVDSIYGHDGFLLESEAVGKLIRKTLELAER"
     gene            6527..7258
                     /locus_tag="VFC01_33315"
     CDS             6527..7258
                     /locus_tag="VFC01_33315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN84536.1"
                     /translation="MTHSRQQRSLSFGAEAAAYERGRPSYPPESIDWLLPADAHDVLD
                     LGAGTGKLTTRLVERGLDVVAVDPIPEMLELLSSSLPDTPALLGTAEEIPLPDDSVDA
                     VLVAQAWHWFDPELAGKEIARVLRPGGRLGLVWNTRDERLGWVKDLGRIIGHEDDPLS
                     HKVTLPEPFVDVERHHIEWTSYLTPQALIDLVASRSYCITSPEKVRTHTLDRVRELLT
                     THPALANTNGLALPYVTVGIRATLA"
     gene            complement(7289..7588)
                     /locus_tag="VFC01_33320"
     CDS             complement(7289..7588)
                     /locus_tag="VFC01_33320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84537.1"
                     /translation="MANHTPDTTKKNVFSPTRTFLAAGGLALGLGSFALFASAGTAAA
                     DGLDATPMPPAVIDDDGFHNYDTSSYPAAPSWPAPASYPADSSWPGSSSHPCCSR"
     gene            7891..>9343
                     /locus_tag="VFC01_33325"
     CDS             7891..>9343
                     /locus_tag="VFC01_33325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN84538.1"
                     /translation="MRLVEAALSGKGSSGIVICGAAGVGKSRVAREALYSAASRGCVT
                     HWAVGTSCARTLPLGAFTTWVPSDGADTIQLVRGVIESLTAAAPGTTVVVGVDDVHLL
                     DDLSIFVVHQIVQRRAAKVVLTVRDGEPIPAGTQELWKAGQFERLDLQPLSPDETTTL
                     LSATLGGSVDAQAAGRLWELVRGNVLYLRHIVEQEVADGRLKQLHGFWQWTGDPNMPC
                     GLVELVESRIGALPADAGAVIDTLAIGEPIKLTALKRIAGPDAVEEADTRGLITLDHY
                     DTGIEVRVAHPLYGEVRRKRAAPTRLRRLRGLVAAELAAADDRDDIQVVIRRATLSVD
                     SDLPPDVGLLCNAAHGAIWLADLALADRLAEAAVRAGAGPEAQFLRAHALSWLGRGKE
                     AEAVLADVPITQLTDTDHARLTYLRASNMLWALADPARAKSIIDEASRTTAGQARSWI
                     DALLTVYWFAMDRPDAATLAATNLALDNLPPIVG"
BASE COUNT         1548 a         3052 c         2982 g         1761 t
ORIGIN      
        1 gcatccacgg ttcacccggc acttcaccac acagctgccg aaggacaccg agatcacgca
       61 cctcgatggc gtcgggcaca tcccgatgta cgaggcgcca cgccgggtcg ccgatctgat
      121 cgtcggcttc gtcgaccgct acgcgacgcc gccgccgcgc aaggccatcg gctaagcggc
      181 gtccagcgcc ttcttgaagt tgcccgcgat ggtgtcgagg aactcctcgc tggtctgcca
      241 cggctgatcc tcgccgatca acagcgccag atccttggtc atctgaccct gctcgacggt
      301 cccgacgacg ctgtcctcca gcgcgttggc gaacttgatc acgtcgggag tgttgtccag
      361 cttgccgcga tgctcgagcc cgcgggtcca cgcgaagatc gacgcgatcg ggtttgtcga
      421 tgtgggcttg ccctgctgat gttggcgata atgccgggtg accgtgccgt gcgcagcctc
      481 cgcctcgacg gtcttgccgt ccggcgacat cagcaccgac gtcatcaggc ccagtgagcc
      541 atagccctgc gcgaccagat ccgactgcac gtcgccgtcg tagttcttgc acgcccacac
      601 ataaccgccc tcccacttca ggcaggaggc gaccatgtcg tcgatcaacc ggtgctcgta
      661 cgtcagcccg ttggcgtcga actgctcctt gtactcctcg tcgaagatgc gctgaaactc
      721 gtccttgaac atgccgtcgt agcccttgag gatcgtgttc ttcgtcgaca ggtagacggg
      781 gtacttctgt tgcagcccat agttaaacgt cgagcgggcg aagtcctgga tggactgctt
      841 gaagttgtac atgcccatca tcacgccgcc atcctcaggg atctcgatga cgtcgtgcac
      901 gatcggctcg ctgccgtcgt ccggggtgaa catcaccgtg aacgttccgg gcttgtccac
      961 cttgaagtta tcggcgcggt attggtcacc gaacgcgtga cggccgatga tgatcggctt
     1021 ggtccatcca ggcaccagtc gcggcacgtt cttgatcacg atcggagcgc ggaaaattgt
     1081 gccgcccaag atgtttcgga tggttccgtt cggcgacagc cacatcttct tgaggttgaa
     1141 ctccttgacg cgggcctcgt cgggcgtgat cgtcgcgcac ttgacgccaa cgccgtgacg
     1201 cttgattgcc tcagcggcgt cgatggtgac ctggtcgtcg gtagcatcac ggtgctcgat
     1261 gccgaggtcg taatagtcca agttcacatc gaggtgcggc aagatgagct gctccttgat
     1321 catcttccag atgatccgag tcatctcatc accgtcgagt tctacgacgg tgccttcgac
     1381 cttgaccttg gccatgtaca gcgtcagctg tgcgctgccg ccagcgtctc gttgagcgtc
     1441 ttgctcggcc gcatcaccgc ttcggtcttc tcgcggtccg ggtagtagta gccgccgatg
     1501 tccaccgaat cgccttgcac ctcagcgagt tcggacacga tagcctcttc cttttcggcc
     1561 agcgtctttg ccagtttggc gaagtgctca gccagctcct gatcctcgga ctgcgccgcg
     1621 agctcctgcg cccagtacag cgcgaggtag aactggctgc cgcggttgtc gagctcaccg
     1681 gtcttgcgcg acgggccctt gtcgttctcc agcagcttgc cgatcgccgc gtcgagcgtc
     1741 gtgcccaaca gcgcggcccg cttgttgccg gtcttgtcgc ccaagtcctc gaagcacgcg
     1801 cccagggcga ggaactcgcc gagcgaatcc caccgcaggt ggttctcctc gaccagctga
     1861 tggacatgct tgggcgccga cccgcccgcg ccggtctcat acatgccgcc gccggccatc
     1921 aacggcacga tggacagcat cttggcgctg gtgcccagtt ccaggatcgg gaacaggtca
     1981 gtcaggtagt cgcgcaggat gtttccggtc gccgcgatgg tgtcctggcc gcggatcagc
     2041 cgctccagcg tgtaccgcat ggcccagacc tgcggcatga tctggatgtg gaggccctcg
     2101 gtgtcgtggt cctccaggta ggccttgacc ttcttgcgta gctccacctc gtgcggacgt
     2161 tcggtgtcca gccagaacaa caccgtcatg ccggaagcac gcgcgcgggt gaccgccagc
     2221 ttcacccagt cccggatcgc cgcgtccttg gtgacgggca tgcgccagat gtcgccctct
     2281 tcgacgttct gcgacagcaa cacctcgccg gtgtcgatgt cgacgatgtc ggcaacgccg
     2341 tcctcgggga tctcaaaggt cttgtcgtgg ctgccgtatt cctcggcctt ctgcgccatc
     2401 aggccgacat tcggcacggt gcccatcgtc gtcgggtcga actggccgtg ggtcttacag
     2461 aagttgatca tctcctggta gatcctcgag aacgtggact cggggttgac cgccttggtg
     2521 tcctttgtcc gtccatcggc gccgtacatc ttgccgccca ggcggatcat cgcgggcatc
     2581 gacgcgtcca cgatcacgtc gctcggtgag tggaagttgg tgatgccctt ggccgaatcc
     2641 accatggcca actcgggacg gtgctcgtgc acgtcgtgga tgtcctggat gatctgctca
     2701 cgttggaatg acggcagctt ctcgatcttg ttgtagaggt cgaccatccc gttgttgacg
     2761 ttgacaccca gttcgtcgag cacttcctgg tgcttttcga aggcgtcctt atagaagacc
     2821 ttcacgcagt ggccgaacac gatgggatgg ctgaccttca tcatcgtcgc cttgacgtgc
     2881 agcgagaaca tcacgccggt cttgtaggcg tcctcgatct cggcctcata gaacgcgaca
     2941 agggccttct tgctcatgaa catgctctcg atgacgtcgc cctcgtccag cgccgtctcg
     3001 ggcttgagca cgatcgtctc gccactcttg gtcttcagtt ccatccgcac tttgcgggcc
     3061 ttgtccagcg tcatcgactt ctcgccgtgg tagaagtcgc cacccttcat cgtggccacg
     3121 tgcgtgcgcg acgcctggga gaactcgccc atgctgtgcg gatgccggcg cgcgtactcc
     3181 ttgacagcct tgggtgcacg gcggtccgaa ttaccttcgc gcagcaccgg attcaccgcg
     3241 ctgcccaaaa tcttgccgta gcgctccttg agctccttct cttcgtcgtt cttcggttgc
     3301 gcgggatagt cgggaaccgc gtagcccttc tcctgcagct ccttgatcgc ggccgccagt
     3361 tgcggcaccg acgcgctgat gttcggcagc ttgatgatgt tggtctccgg gtcctgcgtg
     3421 agctcaccca gttggcccag gttgtccggc acgcgctgct cttcggtcag gtagtcggag
     3481 aactcggcca ggatgcgtgc ggccacggag atgtcactgg tttcgacgtt gatgcccgcc
     3541 ggctcggtaa aggtccgaat gatcggcaaa aaggcgtagg tcgcaagcag cggcgcttcg
     3601 tcggtcagcg tgtagatgat ggtcggctgc tcggcgctca tgtcgttatc tcccggcgtc
     3661 tgtctcggtc ccgtgttgtt tcagcggcta tcagtatcac gtcgtgtatg acgctacgcg
     3721 cgccccaccg gtccagcgct gagcagtctg cttggctact gaccagtagc ttctgcgtcg
     3781 cgacagatgg gtagtttctc ccgatgtctg cggcccgtct ccgcggtgac gatggcggcg
     3841 accgatcgag aacaaaacac gtccttggca cccagccagc ggcatgggat atccttctcc
     3901 tcggtcatga gtgccagcgt caagccccgg cttgctggcc ggcaaccctc caaccgcggt
     3961 ggggtgcccc gggtgatgac caggttgagt agccgctagc ggttaccggc aagcgcgggt
     4021 ccgttgtgac gggccccaga gacagatgga ggtccattcc gtatgagcac gccagaagac
     4081 ccgacggcgc actggtcatt cgagaccaaa caggtccacg caggccagac gccagacgta
     4141 gccaccaatg cgcgggcgct gccgatctac cagaccacgt cgtacacgtt caacagcacc
     4201 gagcacgccg ccgccctgtt cggcctggcc gagaccggca acatctacac ccgcatcatg
     4261 aacccgacgc aggacgtcgt cgagcagcgc atcgccgcgc tcgagggcgg cgtggcggcg
     4321 ctgttcctgg cgtccggcca ggccgccgag cacttcgcga tcctcaacct cgccaacgca
     4381 ggcgaccaca tcgtgtcgag cccccggctc tacggcggca cctacaacct gttccactac
     4441 acgctgccca agatcggcat cgaggtcagc ttcgtcgaga accccgatga tctggagtcc
     4501 tggcgagccg ccgtcaagcc caacaccaag gccttcttcg gcgagaccat ctccaatccg
     4561 cagaacgaca tcctcgacat cccgaacatc gcggctgtcg cgcatgacgc cgctgtgccg
     4621 ctgatcgtcg acaacaccgt cgccaccccg tacctgattc agccgcttgc ccacggcgcc
     4681 gacatcgtcg tgcattcggc gacgaagtac ctcggtgggc acggcgcggc gatcgccggc
     4741 gtgatcgtcg acggcggcac gtttgactgg accaacggca agttccccgg cttcacaacg
     4801 ccggacccga gctaccacgg tgtggtgttc gccgaactag gcccgcctgc gttcgcgttg
     4861 aaagcccggg tgcagctgct gcgcgatctc ggctcggccg ctgcgccgtt caacgcgttc
     4921 ctcgtcgcgc agggattgga gacgctgagc ctgcgcgttg aacgtcatgt ggccaatgcg
     4981 caaaaggtgg cggaattcct ggccgggcat ccggacgtga tctcggtgaa ctacgccggg
     5041 ttgccgacct cgccgtggta cgagctggga aagaagctgg cccccaaggg aaccggtgcg
     5101 gtgctggcgt tcgagttggc cggcggcatc gacgcgggga aggcgttcgt caacgcgctg
     5161 acgctgcaca gccatgtcgc caacatcggt gacgtgcggt cgctggtgat ccatccggcg
     5221 tcgacaacgc atgcgcagct cacccctgag gagcagctgg ccaccggggt gacgccgggg
     5281 ctggtgcggc tcgcggtcgg catcgagggc atcgacgaca tcctggccga cctggagcag
     5341 gggttcgccg cggccaagcc gttcagtgcg tcaacggcgg cggatccgaa cacggcggcg
     5401 gcgttctgat gacggtgatc gacgtgagta ccgcgacgct gccggccgag ggtgaagtcg
     5461 gctatgtcga catcggctcg ctgacgttgg agaacggcgc cgtcatcgac gacgtcacga
     5521 tcgccgtgca gcggtggggc gagctgtcgc ccaatcgcga caacgtcgtc gttgtgctac
     5581 acgcgctgac cggcgattcg cacgtcaccg gtccgaccgg tcctggtgac gagaccgaag
     5641 gctggtggga aggcgtggcc ggacccggcg cgccgatcga caccgaccgg tggtgtgcga
     5701 tcgccaccaa cgtgctgggt ggctttcgcg ggtcgacggg tccgagctcg tctgcacccg
     5761 atggaaagcc ttggggctca aggtttccgc tggtgtcggt gcgtgaccag gtcgaggccg
     5821 acattgccgc actggccgcg ctgggcatca ccgaggtcgc cgcggtcgtc ggcggctcga
     5881 tgggcggtgc cagggcgctg gagtggatcg tcagtcatcc cgacgcggtg cgctcggcgc
     5941 tggtgctggc ggtcggggca cgcgccaccg ccgaccagat cggcacgcag agcacgcagg
     6001 tcgcagccat caagtccgat ccggactggc agaacggcga cttccacggc acgggtcggt
     6061 cgccggacct cggcatggag atcgcgcgac gcttcgcgca tctgacatat cggggcgaac
     6121 acgaactcga caaccggttc ggtaacgacc cccaaggtga cgaggacccg gcgacgggtg
     6181 ggcgctactc ggtgcagagt tatctcgagt atcagggccg caaactgctg aagcgcttcg
     6241 acgccggcac ctatgtggca ctgaccgaag cgctgtcgag tcacgacgta ggccgcgggc
     6301 gcggcggcgt ggagaacgcg ctgcgcggct gccccgtgcc gacggtcgtc ggcggcatca
     6361 cgtcggatcg gctgtacccg ttgcggctcc aggaggaatt ggcggagctg ttgcctggtt
     6421 gtaccgagtt gaacgtggtc gactcgatct acggacacga cggattcctg ttggagtccg
     6481 aggcggtggg caagttgatt cgcaagacgt tggagctggc cgagcggtga cccactcgag
     6541 gcagcagcgt tcactgtcgt tcggcgcgga agccgcggca tacgaacgcg gccgaccgtc
     6601 ctatccgccc gagtccatcg actggctgct gccggcggac gcccacgacg tgctcgacct
     6661 gggcgcaggc acgggcaagc tgacgacgcg gctcgtcgaa cgcgggctcg atgtcgtcgc
     6721 cgtcgacccg atcccggaaa tgctcgagct gttgagcagc tcgctgcccg atacgccggc
     6781 gctgcttggc accgccgagg agatcccgct gcccgacgac agcgtcgacg cggtgctggt
     6841 ggcccaggcg tggcactggt tcgatcccga gctggccggc aaggagatcg cacgcgtgct
     6901 gcggccgggt ggtcgactcg gtttggtctg gaacactcgc gacgaacgtc tgggctgggt
     6961 caaggatctg ggccgcatca tcggccacga ggacgatccg ttgagccaca aggtgacgct
     7021 gcccgagccg ttcgtcgatg tcgagcgcca tcacatcgag tggacgagtt acctgacgcc
     7081 gcaagcgctt atcgaccttg tcgcatcgcg cagttactgc atcacatctc cggagaaggt
     7141 gcgcacccac accctggatc gggtccgcga actgctgacc acccatcctg cgctggccaa
     7201 caccaacggg ctcgcactgc cgtacgtcac ggtgggtatc agggcgacgc tggcctgatt
     7261 acgcagcttc cttgaccggg ctacgttttc accgcgaaca acacggatgt gacgagctcc
     7321 ccggccacga cgagtccgcc gggtaagacg cgggcgccgg ccaagacgga gccgccgggt
     7381 aagacgacgt gtcgtagtta tggaagccgt cgtcgtcgat gaccgctggt ggcatgggcg
     7441 tggcgtcgag tccgtcagca gcggcggtgc cggcgctggc aaacagcgcg aaactaccga
     7501 ggccgagcgc caagccgcct gcggcaagga atgtccttgt ggggctgaag acgttcttct
     7561 tcgtcgtgtc gggcgtgtga ttagccattt gttcgcctcc tagatccgcc taccgagcac
     7621 tcggtgtgga ctggtttgat cgtcgcgccg cgctacgtcg agcaaataag tagcgcacca
     7681 cgtgatttcg acgacccccg acgatcccga ctacgtgcga cgacagttaa ggccgccgct
     7741 tccctagcca gcgggcttat ttgatcggtc ccactacttg cgttccatag gtcatcacgc
     7801 tcgtttgtcg atgccctgcg cgaccgcgac cagcgctcct acgctgtatc tgtgcggttg
     7861 ccatggccgt tgaccggacg cttggaggag atgcgactcg tcgaagccgc actgtccggt
     7921 aagggttcgt cggggattgt gatctgtggc gcggcagggg ttggcaagag tcgcgtcgcg
     7981 cgcgaggcgc tctactcggc agcgtcgcgt ggatgcgtaa cccactgggc ggtcggcacg
     8041 tcctgtgcac ggacactccc cctgggtgct ttcacgacgt gggttccgtc agacggcgcc
     8101 gacaccattc agttggtccg cggcgtgatc gagtcactga ctgcggccgc gccaggcacg
     8161 acggtggtgg tcggtgtcga cgacgtgcac ttgcttgatg atttatcgat attcgtggtg
     8221 catcagatcg tccagcggcg cgccgcgaaa gtggtactca cggtacgcga cggtgagccg
     8281 attcccgctg ggacacaaga actctggaaa gcgggccagt tcgagcggct cgatctgcag
     8341 ccgttgtcgc ccgacgagac gacgacgttg ttgtcggcca cactgggcgg atcggtggac
     8401 gcgcaagccg ccgggcgact ctgggagctc gttcgcggca acgtcctcta tctgcgccac
     8461 atcgtggagc aggaggtcgc cgacggccgg ctcaagcagc tgcatggctt ctggcaatgg
     8521 accggagatc caaacatgcc gtgcggcctg gtggaactgg tcgaatcccg tatcggagcc
     8581 ttgcccgccg acgccggcgc agtcatcgac acactcgcca tcggcgagcc gatcaagctg
     8641 acagcgctca aacgcatcgc cggtcccgac gcggtcgaag aagccgacac ccgtggcctg
     8701 atcaccctcg accattacga caccggcatc gaggtccgcg tggcgcaccc gctctacggc
     8761 gaggtgcgca ggaaacgcgc cgcaccgacg aggctgcgtc gactacgcgg gctggtcgcc
     8821 gccgaattgg ccgctgccga cgaccgcgac gacatccaag tcgtcatccg ccgcgccaca
     8881 ttgagcgtcg actccgacct gccacccgat gtcggcctgc tctgcaatgc cgcccacggc
     8941 gccatctggc tcgcggatct ggccctcgcc gaccggctcg cggaagcagc ggtgcgagcc
     9001 ggagccggac cagaagctca gtttctgcgc gcacacgcat tgtcgtggct cggccgcggc
     9061 aaggaagccg aagcggtgct cgccgacgtt ccgatcaccc aattgaccga cacagaccat
     9121 gcccgactca cctatctgcg agccagcaac atgctgtggg cgctcgccga cccggcgcgg
     9181 gcgaagtcga tcatcgacga agcgtcgcgc accacagcag gccaggctcg cagctggatt
     9241 gacgccctcc tcacggtgta ctggtttgcg atggaccgac cggatgcggc gacgctggct
     9301 gctaccaacc tcgcgctgga caacctgccg ccgattgtcg gca
//
LOCUS       DASRJA010000736         6575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3207444,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000736 DASRJA010000000
VERSION     DASRJA010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3207444"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFC01_33330"
     CDS             <1..214
                     /locus_tag="VFC01_33330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84539.1"
                     /translation="DRDELQQWVTHLDAHGVPHSGIIDTGFGPTVVFRNPDNMQLEFH
                     VHPDTGALQLSDADSPEAQQILSSAQ"
     gene            complement(234..998)
                     /locus_tag="VFC01_33335"
     CDS             complement(234..998)
                     /locus_tag="VFC01_33335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN84540.1"
                     /translation="MYDLPPEIDVQADGALRIITLNRPDSLNSVNDALHTGLAHIWAR
                     LGEDRDARAAVITGAGRAFSAGGDFAYLEELRQDADLRAKTILHGREIVLGMARCRIP
                     VVAAVNGPAVGLGCSLVALSDIVYMKEDAYLADPHVQVGLVAADGGPLVWPLQMSLLH
                     AKEYALTGTRISAQRAAELGLANHVVEDPLAEALACAKKIMELPRQAVESTKRLLNIH
                     LERSVLAVLDYANTAEEQSFQTEDFRNIVTRLNAKK"
     gene            complement(1000..2088)
                     /locus_tag="VFC01_33340"
     CDS             complement(1000..2088)
                     /locus_tag="VFC01_33340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN84541.1"
                     /translation="MDFRDSPEEAAFRDRLRGWLTEQKGKFPTSGDDYWAKQGEWHQA
                     LYEAGFFGTSWPKEYGGQDLPPVYDVIVDEEIAKAGAPARPSLGYLVVAFGHHGSKEL
                     QQRFLPGMINGTERWCQGFSEPGAGSDLASLTTTATRDGDEYVIHGHKIWTSYSDVAD
                     WCLLLARTDKDMPKHRGLSAFVVSMHQPGVEQRPLKMISGVTKEFGQVEFDGARVPAE
                     NMVGAPGDGWKIAMTVVSHEREPSTLGFSARYGKLVRQLASRVDGPAPEELSWAWVQT
                     EMLRLHVRRRLSEQLDGITHGPQGSLDKLLMTWVEQSVGHAALATVGTSDPELFGAYM
                     YSRAQSVMGGTSQIQKNIIASRILGLGV"
     gene            complement(2075..2890)
                     /locus_tag="VFC01_33345"
     CDS             complement(2075..2890)
                     /locus_tag="VFC01_33345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN84542.1"
                     /translation="MDVRLTSEQQQLREAAAKLADDLGPGSVADLDDASRVARLEKAV
                     EATGFRTLRSDGASGVEVAIVAEEFARGLVDVPFLGPVLVDDLRRALGREPSAPTAER
                     ESVDLTKSVAGVVESPPELGELSKEDAGRWRALALAVTCADLVGAARGAHALACEYAK
                     VREQYGATIGSYQAVGHLLAEGLALIEGSISVLRHAAWAVDELPAAEAIESAKVAKIY
                     CARAALTVCETSIQVHGGIGNTWECLAHVYLRRVLTATELWPVKLEELSIGLS"
     gene            complement(2875..4272)
                     /locus_tag="VFC01_33350"
     CDS             complement(2875..4272)
                     /locus_tag="VFC01_33350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN84543.1"
                     /translation="MSQPIALAFEEREYTLPQLDALTSGMATTLENRGIRAGDRVALM
                     SSNRPEFVIALRAIWSLGASAVLLSPAWKRTEVESALALTSPAHAVGDHPVLAELMQM
                     LSLDEPIDPAHRTFETPGADSDALFVFSSGTTGMPKAVRHTHGSFAVAVRHWRDALQL
                     TSADRMQIMTPPSHILGLLNIVMALDTGAWIRLHRRFDIDLMLHHIEKDRITIEMAVA
                     PIALALAAHPKLESYDLSSLRYIMWCATPVTQSVAEAVTDRTGVSWVTAYGTSELPVI
                     ACNEIEGARLDTVGRPVPGVRLRIVSLDDGEPLSPGAVGEIQVRSDSVMAGYLPSEAT
                     AQAFTDGWYRTGDVGYLDADGWLRITDRAKEMIKVRGFQVAPAEIEAVLHGHAAVDDC
                     AVFGVPDPANGEAIVAAVKTCRPVEADELIALVADRLASYKRPSQVVFVAEIPRLPSG
                     KVLRRVLKERLWTSV"
     gene            4385..4702
                     /locus_tag="VFC01_33355"
     CDS             4385..4702
                     /locus_tag="VFC01_33355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZN84544.1"
                     /translation="MTAEPVPTAPAGDGSGQVTIVLDRKKVSVPRVADETLLESARRA
                     GLSPPFSCEAGNCGTCMGKLVEGSATMRVNDALEQDEIDDGYILTCQAVPDTESISVT
                     YDD"
     gene            complement(4690..5745)
                     /locus_tag="VFC01_33360"
     CDS             complement(4690..5745)
                     /locus_tag="VFC01_33360"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN84545.1"
                     /translation="MNQGPLSGIRILEVGVMLAGPYATMLLADLGAEVIKIEPPGGEI
                     SRQVSDSYFASLNRNKQSVCLDLRSEAGRQRLGELVANSHALLVNMKPSAIMRLGLTY
                     EALRTFNERIVCVAMTGFGLDGGDDPAFDYVIQAGTGVASMTGHPDGPPTLPGYSSAD
                     NSTGLAAALGLLAQIVSGRGGQVDVSLRDVMFSQLNYRASAYLNDGAAPQRYPFGAHS
                     YYVPAQLFPTADGYLALFITHDAFWKAFADEAKIDGFPTMAERAAKRDEVLEVVTAAL
                     ASDTAASWQSRLQPLGIPVAAVRTLPEALEATPEALVTAGDFRLVGSPIRIAGYEPDY
                     RPPPLLDEHGVVDGQSS"
     gene            complement(5742..>6575)
                     /locus_tag="VFC01_33365"
     CDS             complement(5742..>6575)
                     /locus_tag="VFC01_33365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003896277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN84546.1"
                     /translation="PNDFDCLTMVSLMGARTSRIELGTAVVPLQAQHPIALARQALSV
                     HAGTGGRLALGVGPSHHWIVRDMLGIPYDKPAAYTRDYLEVLNQALAGPGDVDVENDT
                     FTVHNPTVLGAETPMPVLVAALGPVMLQIAGELADGTVLWMADERAIGDHIAPRITKA
                     ADNAGRKAPRIVAGIPVCLCANSEIDAAKERANRILAEAETSPNYQKLLDRGDARDVG
                     DLTAAGDEEAILARFRRFADAGVTDLSVRLLPIGENRDELIESKYRTREVISQLANEV
                     R"
BASE COUNT         1146 a         2290 c         2040 g         1099 t
ORIGIN      
        1 ggatcgagac gagctgcagc agtgggttac gcacctggac gcccacggtg taccgcactc
       61 gggaatcatc gacaccggat tcggccccac cgtggtcttc cgcaatcccg acaacatgca
      121 gctcgaattt catgtacacc cagacacggg cgcgctgcag ctaagcgatg cggattcccc
      181 agaggcgcaa caaatcttga gcagcgctca gtaggcgccg cagcgaacac gaattacttc
      241 ttggcgttga ggcgggtgac gatgttgcgg aagtcctcgg tctggaacga ctgctcctcg
      301 gcggtattgg catagtcgag caccgccaac accgaccgtt cgaggtgaat gttcaacagc
      361 cgcttggtgc tttcgaccgc ctgtcgtggc aactccatga tcttcttggc acatgccagc
      421 gcctcggcca gcgggtcctc caccacatgg ttcgcaagtc caagctccgc ggcacgctgc
      481 gcggagatac gcgtccccgt caacgcgtac tccttggcat gcagcaggct catctgcagc
      541 ggccagacaa gcggcccgcc atctgcggcc accaagccga cctgcacatg cggatcggca
      601 aggtacgcat cctctttcat gtagacgatg tcgcttagcg ccaccaagct acagcccaag
      661 ccgacggccg gcccattcac cgctgccacc accggaattc ggcagcgggc catacccagc
      721 acgatctcac ggccatgcag gatcgtcttg gcacgcagat ctgcgtcctg ccgcaactcc
      781 tccagatacg cgaagtcgcc gcccgcggag aacgccctgc ccgccccggt aatcaccgcc
      841 gcacgcgcat cccggtcctc acccaagcgc gcccagatgt gcgcgaggcc cgtatgcagt
      901 gcatcgttga cggagttgag cgaatcgggc cggttcagcg tgatgatgcg cagcgcccca
      961 tcggcctgga cgtcgatttc cggtggcagg tcgtacaatt cagactccta atccgagtat
     1021 gcgcgaagcg atgatgttct tctgaatctg gctggtgccg cccatcacgc tctgcgcacg
     1081 gctgtacatg taggcgccga acagctcggg gtcgctagtg cccacggtcg ccagcgcggc
     1141 atggccgacg gactgctcga cccacgtcat cagcaacttg tcgagcgatc cttgtggccc
     1201 gtgcgtgatg ccgtcgagtt gctcggacag ccgcctgcgc acgtgcagcc gcagcatctc
     1261 ggtttgcacc cacgcccacg acaactcttc cggcgccgga ccgtcgaccc gggatgccaa
     1321 ctggcgcacc agcttcccgt atcgagcaga aaatcccagc gtcgacggct cccgctcatg
     1381 gctgacgacg gtcatcgcga tcttccagcc gtcccccggc gcacccacca tgttctcggc
     1441 aggcacccgg gctccgtcga actccacctg cccgaattcc ttggtgacgc cgctgatcat
     1501 cttcagcggc ctctgctcga caccgggctg gtgcatcgag acgacgaatg ccgacaaacc
     1561 gcgatgcttg ggcatgtcct tatcggtccg cgccaacagc agacaccagt cggcgacgtc
     1621 ggagtagctg gtccagatct tgtgcccgtg gatgacgtat tcgtcgccgt cacgggtggc
     1681 cgtagtggtc agcgacgcca ggtcggaccc cgcacccggc tcggaaaagc cctggcacca
     1741 ccgttcggtg ccgttgatca tgccgggcag gaatcgctgt tgcagttcct tgctgccgtg
     1801 atggccaaaa gcgacgacca gatagccgag gctgggccgc gcgggagcgc ctgccttcgc
     1861 gatctcctcg tcgacgatga catcgtagac cggcggtaag tcttggccgc catactcctt
     1921 cggccatgac gtgccgaaga agcctgcctc gtagagcgct tgatgccact cgccctgctt
     1981 ggcccagtag tcgtcgcccg acgtcgggaa cttgcccttc tgttcggtca gccagccgcg
     2041 cagcctgtcc cggaacgcgg cctcctccgg cgaatcacga aagtccaatg gacagctcct
     2101 ccagcttcac gggccacagt tcggtggccg tcagcacccg gcgcaggtat acgtgtgcga
     2161 ggcattccca ggtattgccg atgccgccat ggacctggat cgacgtctca cagacagtca
     2221 atgctgcacg cgcacagtag atcttggcga ctttcgccga ctcgatggcc tcggcggcgg
     2281 gcaactcgtc aacggcccac gcggcgtgcc gcaaaacgct gatcgagccc tcgatgaggg
     2341 caaggccctc ggccagcagg tgcccgacgg cctgataaga cccgatggtg gcgccatact
     2401 gctcgcgaac cttggcgtac tcacaggcca gcgcatgcgc accgcgcgcc gcaccgacca
     2461 ggtcggcgca ggtgacggcc agcgccaggg cacgccagcg accggcgtcc tccttggaga
     2521 gctcgcccag ctctggcggc gactcgacca ctccggcaac gcttttcgtc aggtcgacgg
     2581 actcgcgctc cgcggtcggc gcggatggct cacgaccgag cgcccgcctg aggtcgtcga
     2641 cgagcaccgg cccgaggaat ggcacgtcga ccaggccccg tgcgaactcc tcggcgacga
     2701 ttgccacttc gaccccagat gccccatcgg aacgcagcgt ccgaaatccc gttgcctcga
     2761 ccgccttttc cagccgggcg acccgactcg catcgtcgag atctgcgacc gaacccggtc
     2821 cgagatcgtc ggccagcttg gccgcggcct cccgcagctg ctgctgttcg ctcgtcagac
     2881 ggacgtccac aaccgctcct tcaacactcg gcgcaacacc tttcccgatg gcaggcgtgg
     2941 tatttcggcc acgaaaacga cctggctagg tcgcttatag gacgccagcc ggtccgcgac
     3001 gagcgcgatg agctcgtccg cctcgaccgg gcggcaggtc ttaaccgcgg ccacgatggc
     3061 ctctccgttc gcagggtccg gcacgccgaa caccgcacag tcgtcaacag ccgcgtgccc
     3121 gtgcagcacg gcctcgatct cggcgggggc gacctgaaat ccccgcacct tgatcatctc
     3181 cttggctcgg tcggtgatcc gcagccagcc gtcggcgtcc aggtatccga catcaccggt
     3241 ccggtaccag ccatccgtaa acgcctgcgc cgtcgcctca ctcggcagat agccagccat
     3301 caccgaatcc gaccgcacct ggatctcgcc gaccgcgcct ggcgacagcg gctcgccgtc
     3361 gtccagcgac acgatgcgca agcgcacccc tggcacaggc cgaccaacgg tgtcgagccg
     3421 cgcgccctcg atctcgttgc acgcgatgac cggcaattcg ctggtgccat acgcggtcac
     3481 ccaactcacg cccgttctgt cggtgaccgc ctcggcgacg ctctgcgtaa cgggcgtcgc
     3541 gcaccacatg atgtagcgca gcgacgacag gtcatagctc tccaacttcg gatgggcggc
     3601 cagcgccaaa gcgattggtg caaccgccat ctcgatggtg atgcggtcct tctcgatgtg
     3661 gtgcagcatc aggtcgatgt cgaaccggcg gtgcaatcga atccaggcgc cggtgtcgag
     3721 cgccatcacg atgttgagca gtccgaggat gtgtgacggc ggcgtcatga tctgcatccg
     3781 gtcggccgac gtcagctgca gggcgtcacg ccagtgccgc accgcgaccg cgaatgaccc
     3841 atgggtgtgg cgcaccgctt tgggcatgcc cgtcgtcccc gagctgaaca cgaacagcgc
     3901 atcggaatct gcaccgggcg tctcgaacgt ccgatgcgcg gggtctatcg gctcgtccag
     3961 tgacaacatc tgcatcaact cggcgagtac cggatggtcg ccgacggcgt gcgccggcga
     4021 ggtgagcgcc aaagcgctct cgacctccgt gcgcttccac gccgggctga ggagcaccgc
     4081 cgatgcgccc agcgaccaga tggcccgcag ggcgatcacg aactcgggac gattcgatga
     4141 catgagggcg acgcggtctc cggcccggat cccacggttt tccagggtgg tcgccatgcc
     4201 gctggtcagc gcgtcgagct gcggcagtgt gtattcacgc tcctcgaaag cgagcgcgat
     4261 cggctgactc acacgctgtc ctcactcgat gcggctggta caccctgcgc tactgagaat
     4321 agcgtatcac ttatcgagaa tggtattctc ggaatatcag aaccgatgtg ccaggagagg
     4381 cttgatgacc gcagaaccgg tgccgaccgc gcccgccgga gacggtagtg ggcaggtaac
     4441 gatcgtgctc gatcgtaaga aggtgtcggt gccccgggtg gcagacgaaa cgcttctgga
     4501 aagtgcccgg cgcgccggcc tgtccccgcc gttttcgtgc gaagccggca actgcggaac
     4561 ctgcatgggc aagctcgtcg aaggcagcgc gaccatgcgc gtcaacgacg ccctcgagca
     4621 ggatgaaatc gacgacggat acatcctcac ttgccaggcg gttccggaca ccgagtccat
     4681 cagcgtcacc tacgacgact gaccatcgac gacgccatgc tcgtcgagta acggtggcgg
     4741 ccggtagtcc ggctcatatc ccgcgatccg gatcggactg ccaaccaacc ggaaatcgcc
     4801 cgccgtgaca agcgcctctg gcgtggcttc caacgcctcc ggcagcgtcc gcacggccgc
     4861 gacgggtatg cccaggggct gcagccgtga ctgccaactc gcggcggtgt cggaggccag
     4921 cgcggcggtg acgacttcga gcacctcgtc gcgcttcgcc gcgcgctcgg ccatcgtcgg
     4981 gaagccatcg atcttcgctt cgtcggcgaa ggccttccag aacgcatcgt gggtgatgaa
     5041 cagcgcgagg tacccatcgg ccgtcggaaa cagctgcgcc ggcacgtaat acgaatgcgc
     5101 gccgaacggg taccgctgcg gcgctgcgcc atcattcagg taggccgacg cccgatagtt
     5161 cagctgagag aacatcacgt cccggagcga gacatcgacc tgtccgccgc ggcccgagac
     5221 gatctgcgcg agcagcccca gcgcggcggc cagcccggtc gagttgtcgg cagacgagta
     5281 gccgggcagc gtcggtgggc cgtcgggatg gcccgtcatc gacgcgacgc ccgttcccgc
     5341 ctggatgacg taatcgaacg ccgggtcgtc gccgccgtcc agcccgaagc cggtcatcgc
     5401 gacgcagacg atgcgttcgt tgaacgtccg cagcgcctcg taggtgagac cgagccgcat
     5461 gatcgccgac ggcttcatat tcaccagcag cgcatgcgaa ttcgcgacca gctcgcccag
     5521 ccgctgccgt cccgcctccg accgcagatc cagacacacg ctctgtttgt tgcggttcaa
     5581 actggcgaag tagctgtcgc tgacctgccg cgagatctcc ccgcccggcg gctcgatctt
     5641 gatgacctcg gcgccgaggt cggccagcag catcgtcgcg tacgggccgg ccagcatcac
     5701 gccgacctcg aggatccgga taccggaaag cggtccttgg ttcatcgcac ttcgttggcg
     5761 agctgagaga tcacttctcg cgtacggtat ttcgactcga tcaactcgtc gcggttctcg
     5821 ccgatcggca gcaggcgcac cgacaggtcg gtgaccccgg cgtcggcgaa gcgccggaac
     5881 cgcgcgagga tcgcctcctc gtcaccggcc gcggtcaggt cgccgacatc acgggcgtcg
     5941 ccgcgatcga gcagcttctg atagttgggc gacgtctctg cctcggcgag gatgcggttc
     6001 gcgcgctcct tcgcggcgtc gatctcggag ttggcgcaca agcacaccgg gatgccggcg
     6061 acgatccgcg gcgccttgcg gcccgcgttg tccgctgcct tggtgatccg cggcgcgatg
     6121 tggtcgccga tcgcacgctc gtcggccatc cacaacaccg tcccgtcggc gagttcgcct
     6181 gcgatctgca gcatcacggg tccgagcgcg gcgaccagca ccggcatcgg cgtctccgcg
     6241 ccgagcacgg tcgggttgtg cacggtgaac gtgtcgttct cgacgtccac gtcgccgggt
     6301 ccggccagcg cctggttgag cacttcgaga tagtcgcggg tgtatgcggc cggcttgtcg
     6361 taggggatgc ccagcatgtc gcggacaatc cagtggtgcg acggcccgac acccagcgcc
     6421 agccgcccgc cggtccccgc atgcaccgac agcgcctggc gcgccaatgc gatcggatgc
     6481 tgcgcctgca gtggcaccac ggccgtgccg agctcgatgc gcgacgtgcg cgcacccatc
     6541 agcgacacca tggtgaggca gtcgaagtcg ttggg
//
LOCUS       DASRJA010000737        15624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3552610,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000737 DASRJA010000000
VERSION     DASRJA010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 15624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15624
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3552610"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFC01_33370"
     CDS             complement(<1..329)
                     /locus_tag="VFC01_33370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HZN84547.1"
                     /translation="MSLTASSGTAVVSPAERLEGLFEELAELAGQRNAIDGRIVEIIA
                     EVDRDELWGATGARSVAALVAWKIGSSSANAHTIATVARRLEEFPRCAQGMREGRLSL
                     DQVGVIAA"
     gene            complement(502..672)
                     /locus_tag="VFC01_33375"
     CDS             complement(502..672)
                     /locus_tag="VFC01_33375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84548.1"
                     /translation="MGEFVTGEAARERFRALAEQVEAAYAEMRSACCPRVCRSPIAMT
                     FVGRMMGPRWPC"
     gene            891..1142
                     /locus_tag="VFC01_33380"
     CDS             891..1142
                     /locus_tag="VFC01_33380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84549.1"
                     /translation="MNIKRLIGSVAIAGIVGASTLGIGAGIANAKPAQSNSGAATVQP
                     VDWHGGGHGYGRGHGYGHGYGYGWPGFRWHPWGWGWDRW"
     gene            complement(1147..2628)
                     /locus_tag="VFC01_33385"
     CDS             complement(1147..2628)
                     /locus_tag="VFC01_33385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009478444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HZN84550.1"
                     /translation="MLSLLVRSTAPPMWLGLIVAGAAILVETLLVVVLRSLAPMDTFG
                     VVYLLGALVVAIGWGTGLAAVTSVVSALCFGYFREWPNDSFDPADLRNWVVIGVFLVV
                     ALMANTLAGVARARAAEADLTAERQAALRRVATAVAKGLPPHDVFSAVTTELTQIFST
                     GNALLVRYESDGTAQVLAVCVEAGLPKMPVGERFSHGGDGITGKVWRNRRAARMDSHD
                     DVEGSAAARVRTLGLHSGVGAPIVVDGGLWGAAVVASAVPAAFPLDTEARLTEFADLV
                     AMAVANAQARSDLHASRTRIVTAADDARRRLERDLHDGAQQRLVSLALKARTTELSLP
                     AGQDAVGAQLDEIVQGLGDVSEELRAISRGIHPAILSKGGLGPALRSLACHSAVAVEL
                     ELDVPGRLPDQVEVAAYYVVAEALTNAARHAHAELVKVRVKAADGRLDLVVEDDGAGG
                     ADPANGSGLIGLVDRVEAVGGHLWVNSVAGVGTSLAATIPCAA"
     gene            2771..3010
                     /locus_tag="VFC01_33390"
     CDS             2771..3010
                     /locus_tag="VFC01_33390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84551.1"
                     /translation="MDIRLTRHLVVTVLLAATAVVVAWQVLFTGPGMGDNRYTPVQVS
                     TTGNGGPAMQTQPGPVTRVVSIQQDPLQSGAPNAQ"
     gene            3000..3596
                     /locus_tag="VFC01_33395"
     CDS             3000..3596
                     /locus_tag="VFC01_33395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN84552.1"
                     /translation="MHSNTATPEFETIRARFERDAIPLLDAFYPQAMKMTRNRTEAED
                     LLQETAAKAFAGFHGFRDGTNLGGWLYRILVNTHISDYRKQQRRPAQWLTDEITDGQL
                     LAEARHSSSGLRSAEDHVLDQLGDNDVRQALQQLPEKLRTAVYYADIEGRRFKEIAEL
                     TEAPIGTVMSRLHRGRHRLRFLLAEVARDRGYALSDVA"
     gene            complement(3597..4319)
                     /locus_tag="VFC01_33400"
     CDS             complement(3597..4319)
                     /locus_tag="VFC01_33400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84553.1"
                     /translation="MSTPTYFPGYAVPQGTYNAAPVRHSRARVLLIWFAAIAAVAIAL
                     VCVTLTISKPPARYMCPPSCGHPPTGTPVETNPRFTASDGAFSVSYPAEGTAYKITTK
                     PNGVTADLQAGDGGTLQLFSEPAANRSPEQIAKSLIRRTFPDARTAYKIPNAMVGYHP
                     GYGEAADCWPQGANSSYMKMRVMVMVAVKNDLALVAAAVGPFRQFGPDFGSGKPSGAN
                     LQIALDMGKYVNSFSWRGDPPR"
     gene            complement(4316..5521)
                     /locus_tag="VFC01_33405"
     CDS             complement(4316..5521)
                     /locus_tag="VFC01_33405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZN84554.1"
                     /translation="MTTPSNDDAVPQTECRICKTDVPAGEYCGLCGCHLTPRRGEGPD
                     WLRIKDFGAAPGEHLLQPSLASSLFPHLPPRSRTVFRIGLAVVLVALVVFALLRLPAA
                     LVTVASLGFPFLFLLYLHESDGFQDIPTRTWALTAVLGIGLGVGWVLLTGALVARSYG
                     IALGAGVVGARMLKMGVGIPLGGVILMLMPAVIVRLLRPPTRESLDGFMIGALGSLMF
                     TAAATLTRLAPQFGTGVVSRRPMDSLIVEAGIRGVAVPLTAAAAGGLIGAALWFKRPP
                     SKEKQHPGVVRALLVSFAVAVLAVYLGLGLVDVSHFPQVWMLVLYLAVALLALFLLRV
                     GLHLALLHEAHDEIQSDEPLLCPHCGHVVPDMAFCPACGVATRASSRSSRDARREVRP
                     VRTDQGPGS"
     gene            complement(5518..7104)
                     /locus_tag="VFC01_33410"
     CDS             complement(5518..7104)
                     /locus_tag="VFC01_33410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HZN84555.1"
                     /translation="MVARHFGTLALIAVGVFVLVSDLRPSVSGSAGLIGGPYAYLLAS
                     STDLGPSHRADTQLIASLRDSSRPDALFGWADDNGLSVRWRPGETWAVVEGAAEKVAN
                     AFDVEVHDYRGLRGQVFYASPQQPTIPESLRDEVAEFGRILGYTPHHTSRPSILPLDV
                     PDRGLTPAALLTTYNANKLAAEGNTGKGQTIVFFAFDGFEQADLDTFATTYQLPKFTP
                     EVVGGKPEEARGETTMDLEVAHAVAPDARKVVWNARPTVEGDGGYEKIGQMLEDADRQ
                     YPGAVWSFSIGWGCDKLITAADLAPTRSALKTAHSHGTTAFNASGDLAGLECKGGDDW
                     SSPPGSSDVGLDSVASLPEMVDVGGTTLSTDANGVWQAEQAWFDVPLSQGTGGGVSSL
                     FDRPDWQRTVMSDKEPTRRLTPDVAAVADPFTGVRIVFDQQQLVGGGTSQSAPIWAGL
                     AAVMNQYLLANGGQVLGDLNPLLYRTAAGAPLPGFRDVVLGGNAVDDATPGYDLVTGL
                     GTPDIDNLVRNLLIVQKATQ"
     gene            complement(7142..7408)
                     /locus_tag="VFC01_33415"
     CDS             complement(7142..7408)
                     /locus_tag="VFC01_33415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84556.1"
                     /translation="MGRMARRVAAVAGGAAIVTMVGLTASCAKEEEKAPETTTTTTTT
                     TTTSATPAPPPPAPTEKSINPTGGNLFTPPVKAPPAPTGVPGDN"
     gene            7593..9227
                     /locus_tag="VFC01_33420"
     CDS             7593..9227
                     /locus_tag="VFC01_33420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HZN84557.1"
                     /translation="MYQQVLDPVAHSLAWSSLIAALPLLLLFVMLGVLRVTAWVASVV
                     SLALAIVLAILVYGMPLGQALLAGTEGAAFGFFPILWIVINAIWVYQMTVETGHFDVL
                     RRSFARVSDDQRVQAIIIAFSFGALIEALAGFGTPVAVTSVMLMALGFRPLKAAVLAL
                     TANTAPVAFGAMATPILTLGKVTELPSDTLGAMVGRQTPILALFVPLALVAIVDGRRG
                     LRETWPVALVCGIVFAIGQYATSNFISVPLADVVASLLSAGAVVAMVRVWHPRREQQP
                     AVVAGGAADEPTPEFAQRVANPDSRAEVARAYAPYAIIIAIFVICQISAVKNLLDKAT
                     VKFHWPGLHVVSPKGKPVSLTEFTLNLLTTPGTQMLVAGVLTMIALKLSVPRAVKAYG
                     VTLVQLRFAIATVMLVLALAFVMNLSGQTITLGTWMAAAGGAFALLSPVLGWLGVAVT
                     GSDTSSNSLFGALQVTAANQAGLSDVLMAASNSSGGVLGKMISPQNLAIAAAAVGMNG
                     KEGDIFRRVIVWSVVFLALLCVLSGLQASVLSWMVP"
     gene            complement(9257..9619)
                     /locus_tag="VFC01_33425"
     CDS             complement(9257..9619)
                     /locus_tag="VFC01_33425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN84558.1"
                     /translation="MPDQDAIATALAYQDGIAVLAVGGDIDLATVPALEAAIDEALVA
                     KPTGLVIDLSEVGFLASAGLQALVATHNNVSESAQFAVVANSAATSRPIQLTGLDHIF
                     ELYPTLDEALTAVRTNAE"
     gene            9746..10645
                     /locus_tag="VFC01_33430"
     CDS             9746..10645
                     /locus_tag="VFC01_33430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN84559.1"
                     /translation="MSESTTDFVGTATTHAAELVGEDLLPQLVQHLRQNRTALREEWA
                     RRITEAELLTAMSPEEIFSEATTVYDSYVEVLETGSVEALQDYARDLSERIIPRGVET
                     DEVVGIVLLLRDVLARSLFEKYQTDFDLLNRVLDAYEPAANRIANTVAVGFVQERERI
                     IRQQQEAIRELSTPVLQVREQLLILPIIGVLDSQRARQVTEQLLRAIRANRAKVVVID
                     ITGVPTIDSTVANHLVQTVDASGLMGASVIITGLSSDIALTLVTIGLDLSKMNAVGDL
                     QGGIEEAERLLGYEVTRTGEQNG"
     gene            10668..11057
                     /locus_tag="VFC01_33435"
     CDS             10668..11057
                     /locus_tag="VFC01_33435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN84560.1"
                     /translation="MPVPILKQGPILIASVQAALTDSDAERLRHDLMERVSLYRAHGI
                     IVDVTAIDVMDSFAARSLRTIAHMTRLRGADTVIVGLQPEVAFAMVQLGLMFDDMYTA
                     LDLEEGLALLNRHLESRKPADGRDGGG"
     gene            11047..11457
                     /locus_tag="VFC01_33440"
     CDS             11047..11457
                     /locus_tag="VFC01_33440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HZN84561.1"
                     /translation="MGAEFVVDVNNSDDIVIARQAGHELARQLGFSLTDVTMIATAIS
                     EIARNITSYAGRGEVRVGVQYRDGRQALVVRAEDDGPGIVDIERALEDGYSTGRGLGL
                     GLPGARRLMDRLVVESAPGKGTVVEMWKWIPASA"
     gene            11450..12076
                     /locus_tag="VFC01_33445"
     CDS             11450..12076
                     /locus_tag="VFC01_33445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZN84562.1"
                     /translation="MRRKGWIGPIEWAAARRPLPGEEVCGDFPIAVDVGGTAALFGVA
                     DGLGHGEAAETAALRAEDVVKSCRTEPLDVLLQRCHRGLTDTRGAAITLARIDFATDS
                     LSWIGIGNVTADLVAKSPGGVQIRASALLAGGIVGYRIPHALATHQVSIGPGDLLVIA
                     TDGILEDHVQGIDFAASAMTIAEHILDAYSRESDDALVLAARHRGVSS"
     gene            12073..14259
                     /locus_tag="VFC01_33450"
     CDS             12073..14259
                     /locus_tag="VFC01_33450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZN84563.1"
                     /translation="MTRAADFHAQYAAALRAYLAAEDEDTLSVGHELGRRALQEQISM
                     LEIIENHSRLVSGGDTAVGLQFLLQALAALDVATRGFIDGTRRYEQQRARAENLADRD
                     EFRTALVNSLQEGFFVADRTGAVIEMNDAFADITGYGPEGLPYAWQHPWMADDEGSGD
                     RLAELLAQGTLETEAPIRHRDGHIVWVAMSVNAVTSPGTDGDTGHTYVGTIRDVTAAR
                     AAAERERAVARLATAVSVAKSVGEVLSTMLDECHTTLGVRHVAAIMWLAGEHDPTVHL
                     AGTPPVLQWHDLDAPLRQSLQDARAWLPLTVEPVGWATETGTAHGIVATLSRDTSLWL
                     EFGEPRKVSAQDRQLVTALVGHLSLAMQHVRQFEAARDTSLTLQRSMLAPTELPAGFA
                     VRYEPAVPPLEIGGDWYDVLPIGDDCIGIIVGDCVGRGLSAAAVMGQLRSSARALLLT
                     GASPATLLEHLDSVAAFIPDAFCTTVFLAILDVRAETLTYSSAGHVPAVVAAPDSPPQ
                     LLTDARSVPLAVHSDEPRPQATAALTAGSTLLLYTDGLVERRDEPIDAGIARIADVMA
                     QTMELPVDAVADAILDSLAPAVGYDDDVAIVLYRHSGCELAIEIDAIPARLTDVRRQL
                     IAWLRSASVPEPLAGDIVLVVNEACTNSAEHAYRGQEPGTMRVEARRDDQHIHIKVAD
                     SGSWKTPPADPGTRGRGLLLMRTLSENVDVDGTSHGTTVAMSFAVG"
     gene            14396..14818
                     /locus_tag="VFC01_33455"
     CDS             14396..14818
                     /locus_tag="VFC01_33455"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN84564.1"
                     /translation="MTNLPGTDARRPDPLQIRLPAIPASLAVVRAHLRSWLPTAAVNA
                     ATADDVLLAVGEAASNAVEHAVLGAGRDVQLEVTAQATDTGLALTVRDNGRWHALPSS
                     TRGQRGHGSRLMRALVDTVTITPTPQGTTVEMHKELHS"
     gene            14994..15305
                     /locus_tag="VFC01_33460"
     CDS             14994..15305
                     /locus_tag="VFC01_33460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN84565.1"
                     /translation="MTNSQFQLFAAEAEQPPVVSATGDIDLANVNEFSDVLNRAAADS
                     DAITVDLSDVTYCDSAAVRALFAVAATTKLGLIVPASGTIVTLLSISGLDRVATVVTR
                     D"
     gene            15359..>15624
                     /locus_tag="VFC01_33465"
     CDS             15359..>15624
                     /locus_tag="VFC01_33465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination regulator"
                     /protein_id="HZN84566.1"
                     /translation="MQRILERMQADLIDALGLAITVHDKRRDERPAVLASLGIGADFA
                     DAQLSGIGGPVPDALAYQVPVLSLDLWTDDRWPELTLATLCDRAP"
BASE COUNT         2719 a         5278 c         5129 g         2498 t
ORIGIN      
        1 gccgcgatga cgccgacctg atccagcgaa agtcggccct cccgcatgcc ctgcgcacag
       61 cggggaaact cctccagccg gcgcgccacg gtggcgatcg tgtgggcgtt ggccgatgac
      121 gatccgatct tccaggccac caacgccgcc accgaccgcg cacctgtagc accccacaac
      181 tcgtcgcgat cgacctcggc gatgatctcc acaatgcgcc catcaatcgc attgcgctga
      241 ccggccaact ccgccaactc ctcgaacaac ccctcaagac gctcggcagg gctcaccaca
      301 gccgtgccag acgatgcggt caaggacata acgacatcat cgcagccggg tccgacaaat
      361 ctgggcggcc cgatccgcgc caggtttgtg ccgtcatcat ctgccacgag gtgctcggca
      421 gccgctgcga ccccgccgac aagtggcaca tcaagatcca ccaccgcctc atgggccgcc
      481 aaccgctgat tactgcagcc ttcagcacgg ccacctcggc cccatcatgc gcccaacgaa
      541 cgtcatcgcg atcggtgatc gacacacccg agggcagcac gccgatcgca tctcggcgta
      601 ggcggcctcg acctgctcgg ccagcgcgcg aaaccgctca cgcgccgcct ccccggtaac
      661 gaattcgccc ataccccaac gctatcgacc acacctgcca accagtggcc gccaagcatc
      721 aaccacccca acctgtggac gaaatcccat ctggggataa cccgaccgac acaccagaaa
      781 aatgtcggac ctacctgcta gcggggggcg cgttttgggc gccaaccggg acgacgcctg
      841 ccgccgcgcg gagaagcctg gaggacatgg gatttcgaag gagtcgcgta atgaacatca
      901 aacgattgat tggcagtgtc gcgatcgcag gcatcgtcgg cgcgtccacg cttggcatcg
      961 gtgcgggaat cgccaacgcc aagccggcgc aatcgaattc cggagctgcg acagttcagc
     1021 cggtcgactg gcacggcggc ggccatgggt acggccgggg tcatggttat ggacacggct
     1081 acgggtatgg ctggcccggt tttcggtggc acccgtgggg ctggggctgg gatcgctggt
     1141 gagcccctag gccgcgcacg gaatcgtcgc ggcgagcgag gtgccgaccc cggcaacact
     1201 gttcacccac agatgaccgc cgacggcctc gacgcggtcg accagcccga taagaccgga
     1261 accgttcgcc gggtcggcgc caccggcccc gtcgtcctcg accaccaggt cgaggcggcc
     1321 gtcggcggcc ttcaccctca ccttgacgag ttcagcgtgc gcatgtcgcg ccgcgttggt
     1381 aagcgcctct gcgacaacgt agtacgcggc cacctccacc tgatcgggca gccgaccggg
     1441 aacatcaagt tcgagctcga cggccaccgc cgagtggcac gccagcgatc gcagcgccgg
     1501 accgagtccg cccttggaca ggatcgcggg atgaatgccg cgggagatcg cacgcagctc
     1561 ctctgacacg tcaccgaggc cctgcacgat ctcgtcgagt tgtgcgccaa ccgcatcctg
     1621 accggctggc aaggacagct cggtggtgcg ggccttgaga gcaagggaca ccaggcgttg
     1681 ttgcgccccg tcgtgcaggt cccgttccag ccgcctgcgg gcgtcatccg cagcggtgac
     1741 gatccgagtc cgggatgcgt ggagatctga ccgagcctgt gcgttagcga cggccatggc
     1801 caccagatct gcaaactccg tcagccgcgc ctcggtgtcg agtggaaagg cagcgggcac
     1861 agcggaagcg acaaccgccg ccccccacaa cccgccgtca acgacgatcg gggcgcctac
     1921 gccgctgtgc aatccaagag tgcgaacgcg cgcggctgcc gaaccctcta cgtcgtcgtg
     1981 gctgtccatc cgtgcggcgc ggcggttgcg ccagaccttt cccgtgatac cgtcgccgcc
     2041 gtgtgaaaag cgttccccta caggcatttt tggcagtccg gcctcgacgc agaccgccag
     2101 tacttgagcc gtgccgtcgc tctcatagcg aactaggagc gcgttccccg tggagaagat
     2161 ctgcgtaagt tccgtagtga ctgccgagaa cacgtcgtgg ggcggcaacc ccttcgccac
     2221 cgctgtcgcg acgcggcgga gcgcggcctg ccgctcggcg gtcagatcgg cttctgcagc
     2281 cctggcccgc gccaccccgg ccagcgtgtt cgccatcaac gcgaccacaa ggaacacgcc
     2341 gatcaccacc cagttgcgca ggtcggcggg gtcgaaggaa tcgttcggcc attcgcggaa
     2401 gtagccgaag cacaacgcac tcaccacgga tgtcaccgcc gccaggcccg taccccatcc
     2461 gatcgcgacg accagtgccc cgagcaggta cacgacaccg aaggtgtcca tcggtgccag
     2521 gctcctgaga acgaccacca gaagcgtttc caccaagatc gccgcgcccg cgacaatcag
     2581 ccccagccac atcggcggcg ccgtcgaacg caccagcaac gacaacaccc gtcctggcta
     2641 gctcccaacg ctgcggtgcc gctgtgccca gcctgtgcag ccgctgtagc tgtcaagtgc
     2701 cgctagcggt aatcacaaat gggcgccagc gtcgaagacg cctcctgcag aagccgcgat
     2761 ggtgtcaaac atggacatcc gcttgacgcg ccatctcgtc gtgaccgtcc ttctcgccgc
     2821 gaccgcagtg gtggtcgcgt ggcaggtgct gttcaccggc cccggcatgg gggataaccg
     2881 gtatacgccg gttcaggttt ccactaccgg caatgggggg ccagccatgc agacacaacc
     2941 gggcccggtc acgagagttg tgagcataca acaagatccg ttgcaatcag gagcgccgaa
     3001 tgcacagtaa caccgcaaca ccggagttcg agaccatccg cgcgcgcttc gagcgcgacg
     3061 cgattccctt gctcgacgcg ttctacccgc aggcgatgaa gatgacccgc aaccgcaccg
     3121 aggccgaaga cctgctgcag gaaaccgcgg ccaaggcgtt cgccgggttt cacggctttc
     3181 gcgacggcac caacctgggc ggctggttgt accggattct ggtcaacact cacatcagtg
     3241 actaccgcaa gcaacagcgg cggcccgcgc aatggttgac cgacgagatc accgacggcc
     3301 agctgcttgc cgaggcccgt cactcgtcga gtgggctgcg ctcggccgag gaccacgtgt
     3361 tggaccagct gggcgacaat gacgtcagac aagcactaca acagctaccc gaaaagctgc
     3421 gcacggctgt ctattacgcc gatatcgagg gccgccggtt caaggagatc gcggagctca
     3481 ccgaagcccc gatcgggacg gtgatgtcga ggctgcaccg cgggcggcac cgactgcgct
     3541 tcctgctggc cgaagttgcg cgggaccgcg gctacgccct cagcgatgtc gcgtgatcac
     3601 cgcggtgggt cgccacgcca ggagaagctg ttgacgtatt tgcccatgtc caaagcgatt
     3661 tggagattgg cgcccgacgg cttaccgctg ccgaagtccg gaccgaactg acggaacggg
     3721 cccaccgccg cggcgaccaa cgccaggtcg ttcttgacgg ccaccatcac catcacccgc
     3781 atcttcatgt agctgctgtt ggcgccctgt ggccagcagt cggcggcctc gccgtagccg
     3841 gggtggtatc caaccatcgc attggggatc ttgtacgccg tcctggcatc cggaaacgtc
     3901 cgtctgatca ggcttttggc gatctgttcc ggagaccggt tcgccgcagg ctcgctgaac
     3961 agctgcagcg tgccgccatc gccggcctgc agatcggccg tcaccccgtt gggtttggtg
     4021 gtgatcttgt acgccgttcc ctcggcggga tacgacaccg agaacgcgcc gtcggatgcg
     4081 gtgaaccgag gattggtctc gaccggggtg cccgtcggtg ggtgcccgca gctgggcggg
     4141 cacatgtatc gcgcaggtgg cttgctaatc gtcagcgtca cgcagacgag cgcgatggcc
     4201 accgccgcga tcgcagcgaa ccagatcagc agcacgcgag cacgcgaatg tcgcaccggc
     4261 gcagcgttat acgtgccctg cggcaccgca tagcctggga aatacgtcgg cgtactcatg
     4321 agcctggccc ctggtcggtg cgcacgggtc ggacctcacg ccgtgcatcg cgcgacgatc
     4381 gcgacgatgc ccgcgtcgcg acaccacagg ccggacagaa cgccatatcg ggaacgacat
     4441 gtccgcagtg cgggcacagc aggggctcgt cggactggat ctcgtcgtgc gcctcgtgca
     4501 gcagcgccaa gtgcagtccg acgcgcagca gaaacagcgc cagcagagca acggccagat
     4561 acagcaccaa catccacacc tgcgggaagt gggagacgtc gaccaggcca aggcccaggt
     4621 acaccgcgag caccgccacc gcgaacgaca ccagcagcgc gcgcacgaca ccgggatgct
     4681 gcttctcctt gctcggcgga cgtttgaacc acagtgcggc accgatcaac ccgcccgccg
     4741 ctgccgcggt gagcggcacc gccacccccc tgatgccggc ctcgacgatc aagctgtcca
     4801 tcggtcgcct gctcaccaca ccggtaccga actgcggggc cagccgagtc aacgtcgctg
     4861 ccgcggtgaa catcagcgaa ccaagggccc caatcatgaa gccgtccagc gattcccggg
     4921 tcggtggtcg aagcaaccgc acgatcaccg ccggcatcag catgaggatc accccgccga
     4981 gagggatgcc gacacccatc ttcagcatgc gggcgcccac cacgcctgca cccaacgcga
     5041 ttccgtacga gcgggcaacc aaggcacctg tgagaagcac ccagccgacc ccgagcccga
     5101 tgcccagcac cgcggtgagc gcccatgtgc gcgttgggat gtcctggaag ccatcggact
     5161 cgtgcaggta gagcaggaac aggaagggga aacccagcga ggcgaccgtg acaagcgcgg
     5221 cgggcaaccg caacagcgcg aacaccacca gcgccacgag caccacggcc agcccgatcc
     5281 ggaacacggt gcgggatcgt ggtggcagat gtgggaacag cgagctggcc aacgacggct
     5341 gtagcaggtg ttcgccgggc gcagcgccga agtccttgat gcgcaaccag tccgggccct
     5401 ctccgcgccg gggggtgaga tggcagccgc acagcccgca atactcgccg gccgggacat
     5461 ccgtcttgca gatccggcat tccgtctggg gcacggcgtc gtcattcgac ggggtggtca
     5521 ttgcgtcgcc ttctgaacga tgagcagatt gcgcacaagg ttgtcgatgt ccggggtgcc
     5581 gagcccggtg accaggtcgt agccgggggt ggcgtcgtcg acggcgttgc cgcccaacac
     5641 cacatcgcgg aagccgggca gcggcgcgcc tgcggcggtg cgatacagca gtgggttgag
     5701 gtcgcccagg acctgcccgc cgttggcgag caggtactga ttcatcaccg ccgccaaacc
     5761 ggcccaaatc ggtgcggact gcgacgtgcc gccgccgacg agttgttgtt ggtcgaacac
     5821 gatccgcacc ccggtgaacg ggtcggcgac cgcggcgaca tcgggcgtca gccgacgcgt
     5881 cggctccttg tccgacatca ccgtacgctg ccagtccgga cggtcgaaca aagatgacac
     5941 gccgccgccg gtgccttggg acagcggcac atcgaaccag gcctgttcgg cctgccacac
     6001 gccgttggca tcggtcgaca gcgtggtacc gccgacgtcg accatctccg gcagcgaggc
     6061 gaccgagtcc agcccgacat cggaggaccc cggcggcgac gaccagtcgt caccgccctt
     6121 gcactccaac ccggcgaggt cgccgctggc gttgaatgcc gttgtgccat gcgaatgcgc
     6181 ggtcttcagc gccgagcgcg tcggcgcgag gtcggctgcg gtgatcagct tgtcgcagcc
     6241 ccagccgatc gaaaaactcc acaccgctcc gggatactgg cggtccgcgt cctcgagcat
     6301 ctgcccgatc ttctcgtagc cgccgtcgcc ttccacggtg gggcgggcgt tccacacgac
     6361 cttgcgagcg tcgggcgcaa cggcgtgggc gacctcgagg tccatggtcg tttcgccacg
     6421 tgcctcctcc ggcttcccgc cgaccacttc gggggtgaac tttggcagtt gataggtggt
     6481 ggcgaaggtg tcgagatcgg cctgctcgaa gccgtcgaac gcgaagaaca cgatcgtctg
     6541 acccttgccg gtgttgcctt cggcggccag cttgttggcg ttgtaggtgg tgagtagcgc
     6601 tgccggcgtc agacccctat caggcacatc cagcggcagg atcgacggcc gcgaagtgtg
     6661 gtgcggcgtg tagcccagga tcctgccgaa ctcggcgact tcgtcgcgca gtgactcggg
     6721 gatcgtcggc tgctgggggg acgcatagaa cacctgcccc cgcaggccgc gatagtcgtg
     6781 cacttcgaca tcgaacgcat tcgccacttt ttcggcggcg ccctcgacga cggcccacgt
     6841 ctcacccggc cgccaacgca ccgacagccc gttgtcgtcg gcccatccga aaagcgcgtc
     6901 gggccggctc gaatcgcgca gcgaagcgat caactgggtg tcggcccgat gcgacggccc
     6961 gaggtcggtg gaactggcca gcaggtacgc atacggtccg ccgatcaggc ccgcgctgcc
     7021 ggagaccgag ggacgcagat cggaaacgag cacgaacacc ccgacagcga tgagcgccag
     7081 cgtgccgaaa tgccgggcta ccacaactgc cagcgtacgt gcgagctggc ccgatgcggt
     7141 cctagttatc gccggggacg ccggtcggcg ccggcggcgc cttcaccgga ggcgtgaaca
     7201 ggttgccgcc cgtcgggttg atcgacttct ccgtcggcgc gggcggcgga ggggccggcg
     7261 tggccgatgt agtcgtcgtg gtggtcgtgg tggtcgtcgt ctccggcgcc ttttcctctt
     7321 ccttggcgca ggaggcggtc aagcccacca tcgtcacaat cgcagcacca ccggcaaccg
     7381 ccgcaacacg gcgagccatc cgacccatga tttgtccttc ccgacctgtg ccagcggttt
     7441 tccaagatca atctggccaa cggaactcta atccaatgtt tgatcaactg cacagtgatt
     7501 gcgcagcggc ccctcaggcg aggattgaag atcaccgctt cgggttacac ccccgtagtc
     7561 gtgtgtccag ccgtgcccgc gaggtggtcg ccgtgtacca acaggtgctc gatccggtag
     7621 ctcattccct ggcgtggagt tcgctgatag cagcgctgcc actgctgttg ctattcgtga
     7681 tgctgggcgt gctgcgggtg accgcgtggg tggcctcggt ggtctcgctg gcgctcgcga
     7741 tcgtgctcgc gatcctggtg tacggcatgc cgttgggtca ggctctgctc gccgggacgg
     7801 aaggcgccgc gttcgggttc ttcccgatcc tgtggatcgt gatcaacgcg atctgggtct
     7861 accaaatgac cgtcgagaca ggacatttcg atgtgctgcg gcgttcgttc gcccgggtca
     7921 gtgacgatca gcgggtacag gcgatcatca tcgcgttctc gttcggcgcg ctgatcgagg
     7981 cgctggccgg gttcggcact ccggtggcgg tgacgtcggt gatgctgatg gcgctggggt
     8041 tccggccgct caaggcggcg gtcctcgcgc tgacggccaa caccgcgccc gtcgcgttcg
     8101 gcgcgatggc caccccgatc ctgacgctgg gcaaggtgac cgaactgccg agcgacacgc
     8161 ttggcgcgat ggtgggtcgg cagacgccaa tcctggcgct gttcgtgccg ctggcgctgg
     8221 ttgccatcgt cgacggtcgt cgtggtctgc gggagacctg gccggtggcg ctggtgtgcg
     8281 gcatcgtgtt cgcgatcggg cagtacgcga catccaactt catctcggtg ccgctggccg
     8341 acgtggtggc gtcgctgctg tcggcgggcg cggtggtcgc gatggtgcgc gtctggcatc
     8401 cgcgccgcga gcagcagccg gcggtcgtcg cgggtggcgc cgccgacgaa ccgacgcccg
     8461 aattcgcgca gcgcgtcgcc aatcccgaca gccgcgcgga ggtcgcacgc gcatacgccc
     8521 cctacgcgat catcatcgcg atcttcgtga tctgtcagat ctccgcggtg aagaacctgc
     8581 tcgacaaggc gaccgtcaag ttccattggc cgggcctaca tgtcgtgagc cccaagggaa
     8641 agccggtgtc gctgaccgag ttcacgttga acttgttgac cacaccgggc acccagatgc
     8701 tcgtcgcggg cgtgctcacg atgatcgcgc tcaagttgtc ggtgcctcgg gcggtcaagg
     8761 cgtacggcgt cacacttgtc cagctgcggt tcgcgatcgc gaccgtgatg ctggtgctgg
     8821 cattggcttt cgtgatgaac ctgtccggtc agacgatcac gctcggcacg tggatggccg
     8881 ctgccggagg cgcgtttgcg ttgctgtcgc cggtgctcgg ctggctcggg gtcgccgtca
     8941 ccggttcgga tacgtcgtcg aattcgctgt tcggcgccct gcaggtgacc gccgcgaatc
     9001 aggccgggct gtccgatgtg ctgatggcgg cctcgaatag ctcgggcggg gtgctcggca
     9061 aaatgatctc gccgcagaac ctggccatcg ccgcggccgc ggtcggcatg aacggcaagg
     9121 aaggcgacat cttccgccgc gtcatcgtgt ggagcgtggt gttcctggcg ctgctgtgcg
     9181 tgctgtcggg actgcaggcg tcggtgctgt cctggatggt gccctgaaca gcggacgaaa
     9241 ctaactgtcc gccaggctat tccgcgttcg tccggactgc ggtcagcgcc tcgtccagcg
     9301 tcgggtacag ctcgaagata tggtcaaggc cggtcagctg gatcgggcgg ctcgtcgcgg
     9361 cactgttcgc aacgaccgcg aattgcgcgg actcgctcac gttgttgtgc gtcgccacca
     9421 gcgcctgaag gcccgccgac gcaaggaagc cgacctcgga aaggtcgatg accagccccg
     9481 ttggcttcgc gacgagcgcc tcgtcgatgg cggcttccag tgccgggacc gtggccagat
     9541 cgatgtcacc accgaccgcg agcaccgcga tcccatcctg gtaggcgagc gcggtcgcga
     9601 tcgcgtcctg gtcaggcatg cgccgagcct atcgttccat cgccgtccgc gattacctac
     9661 ttcgccgctt ccgtggtcta cccgggcgct aatgtcagca caggactcgt gcaccaacac
     9721 cgcgcgagtg aagttgggag gccgaatgtc tgaatcgacg acggatttcg tcggcaccgc
     9781 gaccacacac gccgccgaac ttgtggggga agatctgcta ccccagctcg ttcagcacct
     9841 tcgccaaaac cggaccgcgc tgcgtgagga atgggcacgc cgaatcaccg aggccgaact
     9901 gctgaccgca atgtcgccgg aggagatctt ttccgaggca accaccgtgt acgacagcta
     9961 cgtcgaggtg ttggagacgg gtagcgtcga ggcgctgcag gactacgcgc gcgacctctc
    10021 cgagcgcatc atcccgcgag gcgtggaaac ggacgaagtg gtcggcatcg tgctgctgct
    10081 gcgcgacgtg ttggcgcgct cgctgttcga gaagtaccaa accgacttcg acctgctgaa
    10141 ccgagtgctc gatgcctacg agcccgccgc caaccgcatc gccaacacgg tggccgtcgg
    10201 cttcgtgcag gaacgcgagc gcatcattcg ccagcagcag gaggccatcc gcgagctgtc
    10261 cacacccgtt ctgcaggtgc gcgaacagct gttgattctg ccgatcatcg gtgtgctgga
    10321 cagccagcgc gcccgccagg tcaccgagca gctgctgcgc gcgatccgcg ccaaccgcgc
    10381 caaggtggtc gttatcgaca tcactggtgt gccgaccatc gactccaccg tggccaacca
    10441 cctggtgcag acggtcgacg cgtcgggcct gatgggtgcc agcgtgatca tcaccggtct
    10501 gtcgtcggac atcgcactga ccctggtgac catcggcctc gacctgtcca agatgaacgc
    10561 cgtcggcgac cttcagggcg gtatcgaaga ggccgagcga ctcctcggct acgaagtgac
    10621 ccgcaccggc gaacagaacg gatgaccggc cccatcgcca cctcctcatg ccagtaccca
    10681 tcctgaaaca gggcccgatc ctcatcgcct cggtgcaggc cgcactcacc gattccgatg
    10741 ccgagcggtt gcgccacgac ctgatggagc gggtcagcct ctaccgcgcg cacggcatca
    10801 tcgtcgacgt caccgcgatc gacgtgatgg attcgttcgc cgcccggtcg ctgcgcacga
    10861 tcgcgcacat gacgcggctg cgcggcgcgg acaccgtgat cgtgggcctg cagcccgagg
    10921 tggccttcgc gatggtccag ctggggctga tgttcgacga catgtacacc gcgctggatt
    10981 tggaagaggg acttgcgctt ctgaatcggc acctggaatc ccgcaagccg gcggatgggc
    11041 gcgacggtgg gggctgagtt cgtagtcgac gtcaacaact ccgacgacat cgtcatcgcc
    11101 cgccaggccg ggcacgaact ggcccgccaa ctcggcttct cgctgaccga cgtcacgatg
    11161 atcgcaacgg cgatctcgga aatcgcgcgc aacatcacca gttacgccgg ccgaggcgag
    11221 gtgcgcgtgg gagttcagta ccgcgacggc cgacaggccc ttgtggtccg cgccgaggac
    11281 gacggccccg gcatcgtcga catcgagcgc gcgctcgagg acggctattc gacaggacgg
    11341 gggcttggcc taggcctgcc tggcgcgcga cgattgatgg accggttggt cgtggaatcg
    11401 gctcccggta aaggcacggt cgtcgaaatg tggaagtgga tcccggccag tgcgtagaaa
    11461 agggtggatc ggaccgatcg agtgggccgc ggcacggcgc cccctccccg gcgaggaagt
    11521 ctgcggcgac ttcccgatcg cggtcgacgt cggcggcacc gccgctctgt tcggggtggc
    11581 ggacgggctg ggtcacgggg aagccgccga gaccgcagca ttgcgcgccg aggacgtggt
    11641 gaagagttgc cgcacggagc cactcgacgt gctgcttcag cgatgtcatc gcggcttgac
    11701 cgataccagg ggcgcggcaa tcacgttggc ccgcatcgac tttgcgaccg actcgctgag
    11761 ctggatcggt attggcaacg tgacggccga ccttgtcgcc aagagccccg gcggtgtcca
    11821 gatccgcgcg tccgcgctgc tggccggcgg catcgtcggc tatcgcatac cccacgcact
    11881 cgccacgcat caggtgtcga tcggccccgg cgacctgctc gtcatcgcga ccgacggcat
    11941 tctcgaagat cacgtccagg gcatcgactt cgccgcgtcg gcgatgacga tcgccgaaca
    12001 cattctcgac gcttacagcc gggaatccga tgacgcgctg gtgctcgccg cgcgtcatcg
    12061 aggcgtctcg tcatgacgcg tgccgccgac ttccacgccc agtacgccgc cgcgctgcgc
    12121 gcctaccttg ccgcggagga cgaggacacc ttgtcggtcg gccatgagct cggccgccgc
    12181 gccctgcagg aacagatcag catgctggag atcatcgaga accattcgcg gctggtttcc
    12241 ggaggcgata ccgcggtggg gttgcagttc ctgctgcagg cgctggcggc gcttgacgtg
    12301 gcgacgcgcg gattcatcga cggcacaagg cgatacgagc aacagcgagc ccgcgccgag
    12361 aatctcgccg accgtgacga gttccgcacc gcgttggtga actcgctgca ggaggggttt
    12421 ttcgtcgccg atcgcaccgg ggcggtcatc gagatgaacg acgcgttcgc ggacatcacc
    12481 ggctacggcc ccgagggtct gccgtacgca tggcagcacc catggatggc cgacgacgag
    12541 gggtccggtg accgcttggc cgagttgctc gcgcagggca ccctggagac cgaggcgccg
    12601 atccggcatc gcgatggcca catcgtgtgg gtggcgatga gcgtcaacgc cgtcacgtcg
    12661 ccgggcactg acggggacac cgggcacacg tacgtcggga ccattcgcga cgtcaccgcg
    12721 gcgcgggcgg cggctgaacg ggagcgtgcc gtggcgcgcc tcgcgacggc ggtcagtgtg
    12781 gccaagagcg tcggcgaggt gctgtcgacc atgctcgacg agtgccacac gaccctgggc
    12841 gtgcggcatg tggcggccat catgtggctg gcgggcgagc atgacccgac cgtccacctg
    12901 gcaggaacgc cgccggtgct gcaatggcac gatctcgatg caccgctgcg ccagtcactg
    12961 caggacgccc gcgcctggct accgctgacc gtggaacccg tcgggtgggc gaccgaaacc
    13021 ggcaccgcac acggcatcgt cgccacgctg tcgcgggaca cctccctgtg gctggaattc
    13081 ggtgaacccc gcaaggtcag cgcgcaagac aggcaactgg tcaccgcgct cgtcggccac
    13141 ctcagcctcg cgatgcagca tgtgcgccag ttcgaggccg cccgcgacac gtcgttgacg
    13201 ttgcaacggt cgatgcttgc gccgactgag ttgcccgccg gattcgccgt gcgatacgag
    13261 cccgcggtgc cgccgctgga gatcggcggc gactggtacg acgtgctgcc catcggcgac
    13321 gactgcatcg gcatcatcgt cggggactgc gtcggtcgcg ggctctccgc ggccgcggtg
    13381 atgggtcagc tgcgcagctc ggcccgcgcg ctactgctga ccggcgcatc gccggcgacg
    13441 ttgctcgagc acctggattc ggtggcggcg ttcatcccag atgcgttctg caccacggta
    13501 tttctggcga tcctcgatgt ccgggccgaa acgctgacct acagcagcgc cggtcacgtg
    13561 cctgcggtcg tcgccgcccc cgattcgcca ccgcagttgt tgacggacgc gcggtcggtg
    13621 ccgctggccg tacacagcga cgagccgcgc ccacaggcca ccgcggcatt gaccgccgga
    13681 tcgaccctgc tgctctacac cgacggactt gtcgagcggc gtgacgaacc gatcgatgcg
    13741 ggaatcgctc gcatcgccga cgtcatggcg cagacgatgg aattgcccgt cgacgcggtt
    13801 gccgacgcga ttctcgacag cctggctccc gccgtcggtt atgacgacga cgtcgcaatc
    13861 gtgctgtatc gccactcggg ttgcgagctc gcgattgaaa tcgacgcgat tccagcacgg
    13921 ctgaccgacg tgcgacgtca gctcattgcc tggcttcgca gcgcgagcgt gccggagccg
    13981 ctggccgggg acatcgtgct ggtggtcaac gaggcgtgca ccaacagcgc cgagcacgcg
    14041 taccgcggcc aggagccggg aacgatgcgt gtcgaggcga gacgcgacga ccagcacatc
    14101 cacatcaagg tcgccgactc ggggtcgtgg aagaccccac ccgccgaccc tggcaccagg
    14161 ggcagggggc tgctgttgat gcggacgctc agcgagaatg tggacgtcga cggaacatcg
    14221 cacggaacca ccgtcgccat gagcttcgcg gttggataac gacatgtcgt gcacgacgtg
    14281 gatgttcacg tgatatgaca acaacagcaa atttcggctg tcgaggcgag gcaggcgatg
    14341 cgggaagatt cgcatgcgtt caggcggggt cgccgctaga catccggagg tgctggtgac
    14401 aaaccttccc ggtaccgacg cgcgccggcc cgaccccttg caaatccggc tgcccgcgat
    14461 accagccagc ctcgccgtcg ttcgcgccca cctgcgcagc tggctgccga ccgcggcggt
    14521 caacgccgcc accgccgacg atgtcctgct tgccgtcggt gaggcggcct ccaacgccgt
    14581 cgagcacgcc gttctcggcg ccggccgtga cgtccagctg gaggtcacgg cacaggcaac
    14641 cgacaccggt ctagcgttga cggtcaggga caacggccgc tggcacgctt tgccatcgag
    14701 cacgcggggc caacgcgggc acggcagcag gctgatgagg gccctcgtcg acacggtcac
    14761 gatcacgccc accccgcaag ggaccactgt ggaaatgcac aaggagctgc attcatgact
    14821 ttccgaagac gactgcgagt gaggatcgca gcgaggcacg agtccccgaa gacgaccgcg
    14881 agtgaggatc gcagcgcgga acgcgcgagg accggagcga gcgggagtcg aggcaacagg
    14941 cgaggaccgg tgggcccacg cccgaagggc aggggacgag cgggagtcga gcgatgacca
    15001 acagtcagtt ccagctgttc gctgcggagg ccgagcagcc gccggttgtc agtgccaccg
    15061 gcgatatcga tctggcgaac gtcaacgagt tctccgacgt cctgaacaga gcggccgccg
    15121 acagcgacgc catcactgtc gacctgtcgg acgtgaccta ttgcgacagc gcggcggtgc
    15181 gggcgttgtt cgccgtcgcg gctaccacca aactcgggct gatcgtgccg gcatcgggaa
    15241 cgatcgttac gctgctcagc atctccggcc tcgaccgcgt ggcgaccgtg gtgacccgcg
    15301 actgaccatg accccgccgt actttcaacc acgccaacac ccacaactgg tagcgctgat
    15361 gcagcgcatc ctcgagcgga tgcaggccga cctgatcgat gcgctcggcc tggccatcac
    15421 cgtccacgac aagcgtcgcg acgaacggcc ggcggtgctc gcttcgctgg ggattggcgc
    15481 tgacttcgcc gatgcccaac tgtcggggat cggcgggccg gtgcccgatg cgctcgccta
    15541 tcaggtaccg gtgctgagcc tcgatctgtg gaccgacgat cgttggcccg aactgaccct
    15601 tgcgaccctg tgcgaccgtg cgcc
//
LOCUS       DASRJA010000738         4253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3487651,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000738 DASRJA010000000
VERSION     DASRJA010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3487651"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1119)
                     /locus_tag="VFC01_33470"
     CDS             complement(139..1119)
                     /locus_tag="VFC01_33470"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HZN84567.1"
                     /translation="MLAGAAREVTDHLFRSSNPQEDRLALLYLDQIYLRRAAGVPAGV
                     VSASLDHGARLGRALVGWIAADGYAGTLGRPYSAPVGESLWRPSPPNFGTAISPYWSE
                     VRPMVLRSADEVTPVAHVPYSTSVGSPFWQQANTTYQTGLALTQAQRETAMFWRDNPH
                     TSGLPSGHWMQIVRQVCEQQQLSLARSVETYARVGVALHDAFLNCWTWKYRYNLIRPV
                     DYVRRYINPNWLTWVATPPFPEYTSGHSVASAAAAHVLTNLFGDLAFTDQNAIPEWGT
                     RQFGSFREAAQQAATSRLHGGIHYPMAIEAGLDQGAAIGSLVVNRLQTRR"
     gene            complement(1530..>4253)
                     /locus_tag="VFC01_33475"
     CDS             complement(1530..>4253)
                     /locus_tag="VFC01_33475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZN84568.1"
                     /translation="GSESNADFISNPYNFVLFAFEENNPRFGQPAWSGVGGGLPDNSW
                     GTTIVDLSRYAQPGDKIRLRWDMSTDYCFGTDAGWYVDNVKVYTCPVRTVSGTVTGNG
                     QPLGGINVTAYRWSTDSFYWEPTQSSVTGPDGRYALSLLSGTYRIGFIDPQGPYGSVF
                     FDGAGNLDQANDVVVGSDDVATINARMVLNHPITGKASVDGVDMPNVAVTAWRQVPDG
                     SGSTVWDAVKFTTTGFDGTYTLYVPDGTYRVGFATWQGRWPPIYYDGASSVERATDIV
                     VAGAGVPNINAAFLSVIPESGPAITGAVTVVGTGAPVGGITVSAWRDTVVGPVLVRSK
                     ETGPDGTYALYVPEGTYRVDFKHPFNRYSAVFYGDVFVPTEGAPNINAQLVENHYISG
                     TLTADAVPDLPPGPPPTAITASRWNAPINAWEQVSSAFSAPDGSYVLYVPDGTYRIGF
                     APVGPYYPAYYDGAEALAEANDVIMAGGNVSNINAHLRRIVFASPWPAATSLSGAGQD
                     AWGPQVAAGPDGAVTAVWYRRDGRNSPVQVATLAPNEYWSPPTDLSLPGEDASDPQVA
                     MGARGTAVVVWRGWDGSHYRVQASSRGENGKWSTPTSLSKPSEDAWDPQVAMGPHGMA
                     VVAWRRSDGASHQVQASTRVAYGPWSLPVMLSTVGGEAWDPRVAVGLDGTATAVWSRS
                     DGSNERVQATTRAPNGSWSSPVTLSEAGADALNPQVIVGPDGAATAVWRDGGSDRIQT
                     SSRPAKGVWSTSVTLSGVSGNAYDPQLAVDPDGIVTAVWSWWNGDHGTVQTASRLPGK
                     TWSRPKTLSSCCEDAKAPRVAAGPDGSATALWRATPDGLNLHMVAATRTPDGSWSAPI
                     VVSEESLRAYGAQVVAGSDGTVAAVWERRDGADGRVQAVVLMPVPESRRKK"
BASE COUNT          743 a         1413 c         1335 g          762 t
ORIGIN      
        1 taagtgaatc ttctccaccg atcgttcttg cactcgtctt ttgttgacgg gcgcgtcgca
       61 agcatcgccg agtaggttct cggcaggatt acaaaactgt ggccggcgtc aggggcgcag
      121 gatgtcaaag aggcaatctc accgacgcgt ctgcagacgg ttgaccacca gcgacccgat
      181 ggccgcgccc tggtcgaggc cagcttcgat ggccatcggg taatgaatgc cgccgtggag
      241 ccgggacgtg gcggcctgct gcgccgcctc ccggaaggag ccgaattggc gcgtgcccca
      301 ctcggggata gcgttctgat cggtgaaggc gagatcaccg aagaggttgg tgagcacgtg
      361 ggccgccgcg gccgacgcga ccgagtgccc cgaggtgtac tcggggaacg gcggcgtggc
      421 cacccaggtc agccagttgg gattgatgta acggcgcacg taatccacgg gccggatgag
      481 gttgtaacga tacttccagg tccagcagtt caagaaggca tcgtgcaggg cgaccccgac
      541 gcgggcgtag gtttcgactg agcgcgccag ggagagttgc tgctgctcgc acacctggcg
      601 gacgatctgc atccagtggc cggatggcag ccccgacgtg tgcgggttgt cccgccagaa
      661 cattgccgtc tcacgctgcg cctgggtaag cgcgaggccg gtctggtagg tggtgttggc
      721 ctgctgccaa aagggtgaac ccacgctggt tgagtacggc acgtgcgcga cgggagtcac
      781 ctcgtcggcg cttcgcagca ccatcgggcg gacctccgac cagtacggtg agatggccgt
      841 gccgaagttc ggcggactgg gtcgccacag tgactcgccc acgggtgcgc tgtacggtcg
      901 ccccagcgtg ccggcgtacc cgtctgcggc gatccaaccc accagggcgc gaccaagacg
      961 tgcgccgtgg tccagcgagg cgctcaccac accggctggc acgcccgccg cccggcgcaa
     1021 ataaatctga tccaggtaga gcaaggctag tcgatcctcc tgcggattgg aggacctgaa
     1081 gagatggtcg gtgacctcgc gcgccgcgcc cgccaacacg cacgaagggt caatccttcc
     1141 cggcggtgtc tgcggcagcg gccggagtcc attcaactgg ccaaccaggc tgcgcaggct
     1201 gctcgaggag ggcgcaaccg actcgtacat ggcgaccgag agatagcagt agatccgcgc
     1261 cgcattcgtc ggagtgctgt cgttcgcccc ccagacgacg ttgtaaacct cctggagcca
     1321 ggcgtgagct gtgtcaccag ggagtaactc cgggtcgcgt gccgaatcag cagcccacgc
     1381 gcttggccaa cctgtcacgg cggagccagc tatgccaacc acgccagcac cggttagtct
     1441 caagaaactg cgccgactcc attgcactgt tggatctgat gcagccatgg gaaatctgtt
     1501 taggtgggac acatcggttt tcgtcatttt catttttttc tcctgctctc aggcactggc
     1561 atcagcacga ccgcctgcac ccgcccgtcg gcaccgtcgc gccgctccca aaccgcggcg
     1621 acggttccat ctgaccccgc tacgacctga gcgccgtatg cgcgcaggct ctcctcagac
     1681 acgacgatcg gagcggacca cgatccgtcc ggagtccgcg tcgctgccac catatgcaaa
     1741 ttgaggccgt ctggcgtcgc gcgccaaagt gcagtggcgc tgccgtccgg gcccgcggcg
     1801 acccgcggag ccttggcatc ctcgcaacag ctcgaaaggg tttttggcct cgaccacgtc
     1861 ttacccggca gccgagacgc agtctgaacc gtgccatggt cgccgttcca ccaactccat
     1921 acagcggtga cgatcccgtc tggatccact gccagctggg ggtcatacgc atttccgctg
     1981 acgcctgaga gcgtaactga ggtggaccag actcctttcg cgggccgcga ggaggtctgg
     2041 attcgatcac tgcctccgtc ccgccacacg gctgtggctg cgccatccgg tcctacgatc
     2101 acctgtgggt tgagggcgtc cgcgccggct tcggaaagag tcaccggtga cgaccatgat
     2161 ccgttaggcg cccgggtggt cgcctgcacc cgttcattgc ttccatcgga tcgcgaccac
     2221 acggcggtag cggtaccgtc caatcccacg gctacccgcg gatcccaagc ctcgccgcca
     2281 accgtcgaga gcatgaccgg caacgaccac ggtccgtacg ccacgcgggt actcgcctgc
     2341 acttggtgac tggctccgtc cgatcggcgc caagccacga ctgccattcc atgcggcccc
     2401 atggccacct gtggatccca tgcatcctcg ctgggcttcg aaagactggt cggcgtcgac
     2461 cacttcccgt tctcgccgcg gctgctcgcc tgcacccggt aatgggatcc atcccaaccg
     2521 cgccagacca ccaccgccgt gccccgcgct cccatcgcca cctgaggatc cgaggcatcc
     2581 tcgcctggca gcgacaggtc cgtcggcggc gaccagtatt cgttaggcgc cagggtggcc
     2641 acctgtacag ggctgttgcg tccgtcccgt cggtaccaaa ccgcggtgac cgccccatcc
     2701 ggacccgcgg ctacctgcgg accccaggca tcctgaccgg cgccggacag gctggtcgcc
     2761 gccggccacg gtgaggcaaa cacgatgcgc cgcagatgcg cattgatgtt cgatacgttc
     2821 ccgcccgcca tgatgacgtc attggcctcg gcgagcgcct cggctccgtc gtagtacgcg
     2881 gggtaatacg ggccgacggg ggcgaacccg attctgtaag tgccgtccgg gacatagagc
     2941 acgtagctcc catcgggcgc gctgaatgct gagctgactt gctcccacgc gttaattggt
     3001 gcattccacc gcgatgcggt tatagcggtg ggaggcggcc ctggaggaag atccggcaca
     3061 gcgtccgccg tgagcgtgcc gctgatgtag tggttctcga cgagttgtgc attgatgttc
     3121 ggcgccccct cggtcgggac gaagacgtct ccgtaaaaga ccgcgctata ccgattgaag
     3181 gggtgtttga agtcgacgcg gtacgttccc tcgggtacgt acagcgcgta cgtgccgtcg
     3241 ggccctgtct ccttcgacct caccaacacc ggccccacga ccgtgtccct ccaggcgctg
     3301 actgtgatac cgccgacggg tgcacccgtg cctacgacgg tgacggcgcc cgtgatcgcg
     3361 gggcctgact cgggtatcac gctgagaaag gcggcgttga tgttcggcac acccgctccg
     3421 gcaacaacga tgtcggttgc tcgctcgacg ctgctggctc cgtcgtagta gatggggggc
     3481 caccggccct gccatgtcgc gaaccccacg cgatacgtgc cgtccggcac gtacagtgtg
     3541 taggtgccgt caaatcccgt cgtcgtgaac ttcaccgcat cccacacggt cgatcccgac
     3601 ccgtccggaa cctgccgcca ggccgtgacc gccacgttgg gcatgtctac gccgtcgacg
     3661 ctagccttcc cggtgatggg atggttgagg accattcggg cgttgatggt ggccacgtca
     3721 tcgcttccca cgacgacgtc gttggcttgg tccaggttgc cagcgccatc gaagaacacg
     3781 ctcccgtacg gaccctgcgg atcgatgaac ccgatccgat acgtgccgct cagcaagctc
     3841 agcgcatagc ggccgtcggg acccgtcacg gagctctgcg tgggctccca ataaaatgaa
     3901 tcggtggacc agcggtaggc cgtcacgttg atgccgccca ggggctgacc attcccggtg
     3961 accgtgccgc tgacggtccg cacgggacac gtatagactt tcacgttgtc cacgtaccaa
     4021 ccggcgtctg ttccgaaaca gtagtcggtg ctcatgtccc accgcagtcg gatcttgtcg
     4081 ccgggttggg cgtaacgcga gaggtcaaca atcgtggtgc cccatgagtt atctggcagc
     4141 ccaccgccca cgccgctcca ggcgggttga ccaaacctcg gattgttttc ctcgaatgcg
     4201 aagagcacga agttgtaagg attggagatg aaatcagcat tggactccga ccc
//
LOCUS       DASRJA010000739         4946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_389776,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000739 DASRJA010000000
VERSION     DASRJA010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389776"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VFC01_33480"
     CDS             complement(<1..152)
                     /locus_tag="VFC01_33480"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HZN84569.1"
                     /translation="MAQDLTDLQLLHELEPVVEEKLNRHLSMRKDWNPHDYIPWSDGK
                     NYYALGG"
     gene            555..977
                     /locus_tag="VFC01_33485"
     CDS             555..977
                     /locus_tag="VFC01_33485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84570.1"
                     /translation="MKKSIVLSAGSAVAASALMALFGTGVASADDYAGQTYADASSAA
                     SDAGQTVIVASRVGDKVAQDDCIVTGSQSAPFIHGDDFAHVSDTVLFYLNCNAGYASA
                     NMPGPSLASPAGREAKAAADEAAAEEEQSLEEVSTPGA"
     gene            complement(981..1565)
                     /locus_tag="VFC01_33490"
     CDS             complement(981..1565)
                     /locus_tag="VFC01_33490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN84571.1"
                     /translation="MPSGQRRGRWSGVPLQDRQVLRRDELIAAGVELLGSETGPALTV
                     RAVCREAGLTERYFYESFADRDEFVRAVYDDVCARAMSALMSADTPRDAVERFVALMV
                     DDPARGRVLLLAPGVEPVLTRSGAEWMPSFIELLQRKLTRITDPALQAMVATGLIGAL
                     TALFTAYLNGRLAATREQFIDYCVDMLLSRVSSY"
     gene            1667..2674
                     /locus_tag="VFC01_33495"
     CDS             1667..2674
                     /locus_tag="VFC01_33495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZN84572.1"
                     /translation="MSWGHPPPEGQGDGSPSAAPGCPVTAGGYDTVPAPLGPDSLTWK
                     LFGDWRGLLQGPWAGSMQNMHPQLGAAVEQHSIFFQERMPRLLRSLYPIGGVVFDSDR
                     APQTGAEVRDYHIGIKGVDDQGRRYSALNPDVFYWAHSTFFMGTILTAERFGDGLTEA
                     QKRQLFDEHITWYQMYGMSMRPVPKTWEEFQVYWDHMCRNVLENNWAARQVLDLSTMP
                     KHPSLQSIPDLLWRLQLKVLRPFAVWVTVGLYDPAVRELMGHTWSARDERLHWLFGRL
                     VNLSFKLLPKRRRFHPRARAGWDRATGRIPADAPLVHTPARNLPPLDHRDNGMHYCPK
                     V"
     gene            complement(2679..3044)
                     /locus_tag="VFC01_33500"
     CDS             complement(2679..3044)
                     /locus_tag="VFC01_33500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HZN84573.1"
                     /translation="MLYQQLHRSFWRRHPVVTAAAVVLPCWLLLNVLLNGGAMVAVVV
                     MVGLFIVVRRRVRALEVRDAGLRARADYEHRLRLAGDPRGLYGRYPPVQAGWFPDPQN
                     RSQMRYFDGALWTPYARPR"
     gene            3136..3759
                     /locus_tag="VFC01_33505"
     CDS             3136..3759
                     /locus_tag="VFC01_33505"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZN84574.1"
                     /translation="MTWQVFAAMLLVIVTVIALLMRATSKNDTGRTRATRIAVVAGGL
                     AIFVLILGSTTIVSTRNIGVVTTFGRPSGTLSNGLHAKAPWQSVTEMNGTIQIDNHTG
                     DAATTVRLGNNSTAFVDNSVRWRIQPAAADELFLDYREFDNVRDNLVTRELRATLNEV
                     FADFDPLAPENSDGANVQALGDDVAEKLRTKVGGQIEIINVIVPLVN"
     gene            3843..4001
                     /locus_tag="VFC01_33510"
     CDS             3843..4001
                     /locus_tag="VFC01_33510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84575.1"
                     /translation="MAEAKANEVLAASVSNDPNVLVRQVLDAAREAHISPLGCWPNTT
                     AVPTVPAR"
     gene            4054..4335
                     /locus_tag="VFC01_33515"
     CDS             4054..4335
                     /locus_tag="VFC01_33515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84576.1"
                     /translation="MLRVTQSLRGAVMAFVAAVTIGLASSICAPTPASAVSPMTDAHT
                     QSPSPLIVLVTPFAVGLHVIEAIAEGIANAVEMIATPDPIAIPAPDTST"
     gene            4376..>4946
                     /locus_tag="VFC01_33520"
     CDS             4376..>4946
                     /locus_tag="VFC01_33520"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZN84577.1"
                     /translation="MQAPEVLGGRYELRGVLGRGGMAEVRDGWDIRLDRPVAVKLLYP
                     LFATQPDNRMRFDVEARAAAGLNHPHIVSVHDSGEHAGTPYIVMERLSGQTLADVIAA
                     GPLPQPQVRSILDAVLSALAAAHAAGILHRDIKPANILFSASGDTKVADFGIAKSAAS
                     AHTMTGQIVGTMAYLSPDRIAGRPASVADD"
BASE COUNT          900 a         1637 c         1557 g          852 t
ORIGIN      
        1 ccaccaagtg cgtagtagtt cttgccatcc gaccacggga tgtagtcgtg cgggttccag
       61 tccttacgca tcgacaggtg ccggttgagc ttctcctcaa cgacgggctc gagctcgtgc
      121 agtagctgaa gatcggtcag atcttgcgcc atggcccctc ccagttatct gtacctgttg
      181 gttgcagtca atatatctgt gtaccccggt gacattcaag ttgtccgtcg gagtgtcaca
      241 tctgcctcac aagccaccgt tgtcgccgta ctatctgcgc gggcctttcg gggagcccgg
      301 atttcctgtc gcagtcacat tcagactcgg aggtcgggct cacgtgaaga tcctcaccac
      361 tctcttgtgc gctgccgcag gatttgcagc aattccttcg gtgggcgcgg gcgtcgcggc
      421 cccagaggat cgtcaggcat tgatcgacga ggaggccgga tgacgcgcgc ggtagcggtt
      481 atttggaccg cggaatacac ttccgccatc accgtttggc aggcattact cagacaattc
      541 ggaggtcgga gtcagtgaag aagtcgatcg ttctgtcggc cggttcggcc gtggccgcat
      601 cagccctcat ggctcttttc ggcaccggcg tggcctcagc ggacgattac gcaggccaga
      661 cgtatgccga tgcctcatca gcagccagcg acgccggcca gaccgtcatc gtcgccagca
      721 gggtaggtga caaagtggcg caagacgact gcatcgtcac gggttcgcag agcgccccat
      781 tcatccacgg agatgatttc gcacacgtct cggatacggt gctcttctac ctgaactgca
      841 acgccgggta tgcgtcggcc aacatgcccg ggccgtcgct ggcaagcccg gcgggtcgcg
      901 aggccaaggc cgccgccgac gaggctgccg ccgaagaaga gcagtcgctc gaagaggtca
      961 gcactcccgg cgcgtagtcg tcagtagctc gacacccggc tgagcagcat gtcgacgcag
     1021 tagtcgatga actgctctcg cgtcgccgcc aggcgtccat tgagataagc ggtgaacaac
     1081 gccgtcagcg cgccgatcaa accggtggcc accatggcct gcagagcggg atctgtaatg
     1141 cgggtcagct tgcgctggag caactcgatg aagctcggca tccactcggc gcctgaacgg
     1201 gtgagcacgg gttccacccc gggggccagt aacagcaccc gcccgcgggc ggggtcgtcg
     1261 accatcagcg cgacaaagcg ctcgaccgcg tcacgcgggg tgtccgcgct catcagcgcc
     1321 gacattgccc gcgcgcacac atcgtcgtag accgcgcgga cgaactcgtc gcggtcggcg
     1381 aagctctcgt agaagtagcg ctcggtcaac ccggcctcgc ggcagactgc acggacggtc
     1441 aaagccggcc cggtttcgct gcccaacagc tcgaccccgg cggcgatgag ttcgtcgcgg
     1501 cgcaagacct ggcggtcctg cagcggaacg ccagaccatc ggccccgtcg ttgaccggaa
     1561 ggcacatcac tccctaagct ctcggtgaca acgtacgtag tcagaatcgc ttctggctcc
     1621 gattggaagt tcaccagtga ctcaagatac gtctgaggcg tgccccgtgt cctgggggca
     1681 ccccccgccc gaagggcagg gggacggatc cccctctgcc gcgccaggct gcccggtcac
     1741 cgcgggtggg tacgacactg ttccggcacc gctcggcccg gactcgctga cctggaaatt
     1801 gttcggcgac tggcggggtc tgctgcaggg cccgtgggcg ggctcgatgc agaacatgca
     1861 tccacagctc ggcgcagccg tcgaacaaca ctcgatcttt ttccaggagc gcatgccccg
     1921 gctgctgcgg tcgttgtacc ccatcggggg cgtcgtcttc gacagcgacc gggcgccaca
     1981 gacgggcgca gaggtgcgtg actatcacat cggcatcaag ggcgtcgatg accaaggccg
     2041 tcgctacagc gcgctgaatc ccgatgtctt ttattgggcg cactcgacgt tcttcatggg
     2101 caccatcctt accgccgaac gctttggcga cgggctgacc gaggcgcaga agcggcagct
     2161 gttcgacgag cacatcacct ggtaccagat gtacggcatg agcatgcgac cggtgccgaa
     2221 gacctgggag gaatttcagg tctactggga tcacatgtgc cgcaacgtgt tggagaacaa
     2281 ttgggcagcg cgccaggtac tcgatctgtc gacgatgccc aaacaccctt cactgcagtc
     2341 gattccagac ttgctatggc gtctgcaact gaaagtgctt aggccttttg cagtttgggt
     2401 caccgtcggc ctgtacgacc cggcggtgcg agagctgatg ggccacacgt ggtcggcgcg
     2461 agacgagcgg ctgcactggc tcttcggcag actggtcaac ctgtccttca agctgctgcc
     2521 gaagcggcga cggtttcatc cccgagcccg cgccggctgg gatcgcgcaa cggggcgcat
     2581 ccctgccgac gcgcctctgg tgcacacacc cgcacgcaat ctgccgccac tcgatcaccg
     2641 ggacaacggc atgcactact gcccgaaggt ttagcgcgtc agcgtggccg tgcgtacggc
     2701 gtccacaacg cgccgtcgaa ataccgcatc tgagaacggt tttgcggatc ggggaaccag
     2761 cccgcctgca ccggcgggta gcggccgtac aacccacgtg gatcgccggc cagcctgagc
     2821 cgatgctcgt aatcggcgcg agcccgcaac ccggcgtcgc gcacctcaag agcgcgtact
     2881 cgtcggcgaa caacgatgaa caggccgacc atcacgacga cggcgaccat tgcaccgcca
     2941 ttgagcaaga cgttcagcaa cagccagcac ggcagcacca ccgctgcggc ggtcaccacg
     3001 gggtgccgcc gccaaaacga cctgtggagt tgctggtaga gcatcgcctt tgatcatacg
     3061 attcggcgga cgtttcctgc cgtggatcga ctcacggccc gatcgggccc tgccctcgac
     3121 ggcaggatga cccaagtgac ttggcaagtg tttgcagcga tgctcctcgt gatcgtcacc
     3181 gtgatcgccc tgctgatgcg ggccacatcg aaaaacgaca caggccggac gcgggcgacg
     3241 cgaatcgccg tcgtggccgg tggcctggcg atcttcgtgc tgatcctcgg ctcgaccacg
     3301 atcgtgtcga cccgcaacat cggtgtcgtc acgacgttcg gccgtcccag cgggacgctc
     3361 tccaacggcc tgcacgccaa ggcgccgtgg cagtcggtca ccgagatgaa cggcacgatc
     3421 cagatcgaca accacaccgg cgacgcggcc accacagtcc ggctgggcaa caactcgacg
     3481 gcattcgttg ataacagcgt gcgctggcgc atccaacccg cggccgccga cgaactcttc
     3541 ctcgactacc gcgagttcga caacgtccgc gacaacctgg tgacccgtga gctgagggcg
     3601 acgcttaacg aggtgttcgc cgatttcgat ccgctcgccc cggagaactc cgacggcgcc
     3661 aacgtccagg cgctgggtga cgacgtggcc gaaaagctgc ggaccaaggt cggcggtcag
     3721 attgagatca tcaatgtgat tgtgccgctg gtcaattaag acaggccacc caggaccgga
     3781 tcaacgcact gaacgttgag aaggcaaaca cccgcgtagc cgaacagcgc gccaagaccg
     3841 ccgtggccga ggcgaaggcc aacgaggttc tggccgcgtc ggtgtccaac gatcccaacg
     3901 tcctggtcag gcaagtgctg gacgccgcgc gcgaggcgca catcagccca ctgggctgct
     3961 ggccgaacac cacggcggtg ccgacggtgc cagcccggta acattcggcg tttttcgcta
     4021 tgcctggagc gatttatggg gcacgattct catgtgttgc gcgtgaccca gtcccttcgc
     4081 ggtgcggtga tggcgttcgt cgccgctgtg accatcgggc tggcgtccag catctgtgcg
     4141 cccacgcccg cctcggccgt ctccccgatg accgacgccc acactcagtc accgtcgccg
     4201 ctgattgtgc tcgtcactcc gttcgcggtc ggtttgcacg tcatcgaggc gatcgccgag
     4261 ggcatcgcga acgctgtcga gatgatcgcg acgccggacc caatcgccat ccccgcgccg
     4321 gataccagca cctagcggtt gtttgacgcc ggcgcagacc ggctagcttt aacccatgca
     4381 ggcgccggag gtacttggcg gccgctatga actgcggggt gttctgggac gcggcgggat
     4441 ggccgaggtc cgcgacggat gggacatccg gctcgaccgc cctgtcgccg tcaagctgct
     4501 gtacccgctg ttcgccacgc agcccgacaa ccggatgcgc ttcgacgtcg aggcgcgcgc
     4561 tgccgccggg ctgaaccatc cgcacatcgt gtccgtccac gacagcgggg aacacgccgg
     4621 cacgccgtac atcgtgatgg agcggctgtc cgggcagacg ctcgccgatg tgatcgccgc
     4681 gggaccgttg ccgcagccgc aggtgcggtc aattctggac gccgtgctgt cggcgctggc
     4741 cgcggcccac gcggcaggca tcctgcatcg cgacatcaag ccggcgaaca tcttgttctc
     4801 ggcctccggc gacacgaagg tggccgactt cggtatcgcg aaaagcgcag caagcgccca
     4861 tacgatgacg ggccaaatcg tcggcaccat ggcttatctg tcccccgacc ggatcgccgg
     4921 caggccggcg tccgtggccg atgatc
//
LOCUS       DASRJA010000740         7817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2075197,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000740 DASRJA010000000
VERSION     DASRJA010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075197"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VFC01_33525"
     CDS             <1..1033
                     /locus_tag="VFC01_33525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010844609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methane monooxygenase"
                     /protein_id="HZN84578.1"
                     /translation="FVAMPDEAAANGDYLLPTVFHSVQSDESRHISNGYSILLMALAD
                     ERNRPLLERDLRYAWWNNHCVVDAAIGTFIEYGTKDRRKDRESYAEMWRRWIYDDYYR
                     SYLLPLEKYGLTIPHDLVEEAWKRIVDKHYVHEVARFFATGWPVNYWRIDAMTDKDFE
                     WFEEKYPGWYNKFGRWWEDYNRLAYPGRNKPIAFEEVGYQYPHRCWTCMVPALIREDM
                     VVDKVDGQWRTYCSETCHWTDAVAFRSEYEGRPTPNMGRLTGFREWETLHHGKDLADI
                     VSDLGYVRDDGKTLIPQPHLDLDPKKMWTLDDVRGNTFNSPNVLLNEMSDVERDAHVS
                     AYRAGAVPA"
     gene            1121..2164
                     /locus_tag="VFC01_33530"
     CDS             1121..2164
                     /locus_tag="VFC01_33530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZN84579.1"
                     /translation="MADIHRVNFEPVDIEMEVGEEENILDAAFRQGIHLMHGCREGRC
                     SACKSYVLDGDIQMDNYSTFACNDAEVDEGYVLLCRSHAFSDCTIELLNFDEDELLGG
                     LPIQDVRTRVTAIEPVTKDIVSLQLETVEPAAFEFKPGQYADIHIPGSDEHRSFSMAT
                     TPSTPGGIEFLIKKYPGGKFSGLLDDGITAGDELSLTGPYGTSTLKEGHVLPVVCVAG
                     GAGMAPILSILRHLGETGSRRPVRFYYGARTASDLFYLDEIAEIGRGLVDFVFTACLS
                     ESTEGALPDTLTVEEGNVTDVVARHEAEIGKTEVYMCGPPPMVDAALDLLQTHAVPKD
                     QIFYDKFTSPIFD"
     gene            2208..3329
                     /locus_tag="VFC01_33535"
     CDS             2208..3329
                     /locus_tag="VFC01_33535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006370698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic/alkene monooxygenase hydroxylase
                     subunit beta"
                     /protein_id="HZN84580.1"
                     /translation="MSAPAKQRSFPKIEFTDSEAGALEFPSSKSRKFTYYKPAKLRST
                     MYEDVTIDVQPDPERHLSQGWIYGFGDGPGGYPQDWTVAKSSNWHAFLDPNEEWEQTI
                     YRNNAAVVRQVELCLRNAKRARAYDGWNSSWLKFLECNLGAWMHVENGLALHVFTSIQ
                     RSGPTNMINTAVAVNAAHKLRFAQDLALFNLDLSEAAGPPKGFDGSAHKEVWQSAPEW
                     QLTREVTERLTAVGDWCELLFATNIVFEQLVGQLFRSELVMQIAARNGDYITPTIVGT
                     GENDYDRDLAYTRNLFRILTRDEQFGESNKELFSSWLSIWVPRCLAAAQALQPLWSQP
                     AEKVVTFATSLGHVESQFAGLLESIDLSAPEATIKEDDK"
     gene            3326..3667
                     /locus_tag="VFC01_33540"
     CDS             3326..3667
                     /locus_tag="VFC01_33540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009472934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmoB/DmpM family protein"
                     /protein_id="HZN84581.1"
                     /translation="MTMQFGSATEVSNMCGVTLMNTPVGRVVADVMGAKDGVELTEYP
                     SMIRVDGQRVLDFDYAELTEALGEEFDGSIFEEISSTHYGRMVHLDDRTLLFANPEDA
                     AEYIGFDLTAK"
     gene            3689..4732
                     /locus_tag="VFC01_33545"
     CDS             3689..4732
                     /locus_tag="VFC01_33545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN84582.1"
                     /translation="MYTKDGNSYFIVDGHVHIWDGRAANHRNIHGKQFIDCFYDYHRN
                     LSPEDVVWDYDTYTYYGGERLMRDLFAEGYVDHAIFQATLLSDFYVNGFGQTEEAFAL
                     TRQHPDKLTYNHAYDPRNGEAGLVQLRRDTERMKLQGVKLYTAEWHGDSRGYKLDDPW
                     SRRYLEECIALGIRNIHVHKGPTIRPLDRDAFDVADIDKVATDYLELNFIVEHVGLPR
                     LEDFCWIATQESNVYGGLAVAIPFIHTRPRYFAQIIGELLYWIGDDKILFASDYALWT
                     PKWLVEKFVDFQIPEDMAEYAPITVEQKKKILGLNAAALYDINVPTELQLAEAAGQRG
                     VEVAAGAKEAVPL"
     gene            4729..5466
                     /locus_tag="VFC01_33550"
     CDS             4729..5466
                     /locus_tag="VFC01_33550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HZN84583.1"
                     /translation="MTVATVSIESEILAALDTVTDPELDQPITGLGFVRSVSIDDDGV
                     TVHLRLPTAFCSPNFAYLMAADAQDALRTVDGIGEVRVLLDDHHDSDKINAGLAAEAG
                     YVGTFGAEAEQSLDQLRRTFLSKAHIAAMERCVTALMRQNALHPNEIHRLTLRDLPDG
                     REKTALLRRRFALGLSVCPNGRIVVDDAGRTLASEAVPMRLRFARSVRISMEGNAHFC
                     RGLLATRYDDGGTATPQFTNTRTRSPR"
     gene            5463..6488
                     /locus_tag="VFC01_33555"
     CDS             5463..6488
                     /locus_tag="VFC01_33555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005242373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZN84584.1"
                     /translation="MRAVQVVGYHTKLQLIDIPEPTIEKPLDVIVRIGGAGVCRTDLH
                     ILEGQWEAKSGVELPYTIGHENAGWVHAIGDAVTNVKVGDKVILHPLITCGLCRACRF
                     GDDVHCENSQFPGIDTNGGYAEYLRTTARSVVKIDDVLEPADVAALADAGLTAYHAAA
                     KVACVTRPGNVCVVIGAGGLGHIGIQVLKAISGVTVVVVDRNPDAVKLAKDIGADQGI
                     VADGSHVQQILDLTGGHGAEAVLDFVGEGGATKEGVAMLRRAGNYFVVGYGENIDVPT
                     IDIISTEINFIGNLVGSYNDLQELMVLAAQGKVKLHTSEYPLDEFQRALDDLDAGRVR
                     GRAILVP"
     gene            6508..>7817
                     /gene="groEL"
                     /locus_tag="VFC01_33560"
     CDS             6508..>7817
                     /gene="groEL"
                     /locus_tag="VFC01_33560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZN84585.1"
                     /translation="MAKELRYNTEARTRLEYGVNALADAVKVTLGPKGRNAVLEKLTG
                     PPTITNDGVTIAREIQLSDPFANMGAQLVKEVAMKTNGVVGDGTTTATVLAQAMVREG
                     LRAVEAGANPMRVRRGIERAVPVVLQFLTEHANPVNGRNDLLHIATLAASDDEVIGEV
                     ITRAVEHVGEIGVITTEESDALGLTVDVVDGIEFDHGYISAYMVTNPERMEAALDNPL
                     ILLTNKKITAVQEIMPTIEVAKRADRPLMVLAEDVDGPALQLLVGGNMHKTMLSVVVR
                     APGFGHRRVAELEDLAVALGGHVIAKDTGVELSEVTLQHLGSCDRITVTEHETTIVGA
                     HGEQRLIDARVGQLEAQRERARIDADRDSLDLRIARLTGRVAVIRVGGATSVELKERM
                     LRVDDALAATRAAVEAGIVSGGGTALAQAHRALADLTLPGDDAIG"
BASE COUNT         1549 a         2543 c         2440 g         1285 t
ORIGIN      
        1 tttcgtcgcg atgccggacg aggccgccgc gaacggggac tatctgctgc cgacggtgtt
       61 ccactcggtg cagtccgacg agtcacggca catctccaac ggctactcca tcctgctgat
      121 ggcgctggcc gacgaacgca accggccact gctggaacgc gacctgcgct atgcgtggtg
      181 gaacaaccac tgcgtggtgg acgccgcgat cggcacgttc atcgagtacg gcacgaaaga
      241 ccggcgcaag gaccgggaga gctatgccga gatgtggcgt cgctggatct acgacgacta
      301 ctaccgcagt taccttctgc cgctggagaa gtacgggttg acgattccgc acgatctcgt
      361 cgaagaggcg tggaagcgca tcgttgacaa gcactatgtg cacgaggtcg cgaggttctt
      421 cgccaccggc tggccggtca actactggcg catcgacgcc atgaccgaca aggacttcga
      481 gtggttcgag gagaagtacc cgggctggta caacaagttc ggtcggtggt gggaggacta
      541 caaccggctc gcctacccag gccgcaacaa gccgatcgca ttcgaagagg tcggatacca
      601 gtacccgcac cgatgctgga cgtgcatggt gcctgcgctg atccgcgagg acatggtcgt
      661 ggacaaggtt gacggccagt ggcggaccta ctgctcggag acctgccact ggaccgacgc
      721 ggtcgcattc cgcagtgagt acgagggcag gccgacgccg aacatgggac ggctcaccgg
      781 attccgggaa tgggagacgc tgcaccacgg caaggacctt gccgacatcg tctctgacct
      841 gggttacgtg cgcgacgacg gcaagacgct gatcccgcag ccgcatctcg atctcgaccc
      901 gaagaagatg tggacgctcg acgacgtccg cggcaacacg ttcaacagcc ccaacgtgct
      961 gctcaacgag atgtccgacg ttgagcgcga cgcccacgtc tcggcgtacc gcgcaggcgc
     1021 cgtccccgcc tgacgcacgc cggtgggcgg cgcccctccc accccttcgg cgccgcccac
     1081 cggatcccaa cgcaaccatg actgccggag gagaaattcc gtggccgaca tccaccgcgt
     1141 caacttcgag cccgtcgaca tcgaaatgga ggttggcgag gaggagaaca tcctcgacgc
     1201 tgccttccgg cagggcatcc acctgatgca cggctgtcgc gaaggccggt gctcggcttg
     1261 caagtcctac gtgctcgacg gcgacatcca gatggacaac tactcgacct tcgcctgcaa
     1321 cgacgccgag gttgacgagg gctatgtgtt gctgtgccgc tcacatgcgt tcagcgactg
     1381 cacaattgaa ctgctgaatt tcgacgagga cgagctgctc ggcggcttgc ccatccagga
     1441 tgttcgcacc cgggtgaccg caatagagcc ggtgaccaag gacattgtgt cccttcaact
     1501 ggaaacggtg gagccggccg ccttcgagtt caagcccggc cagtacgccg acatccacat
     1561 ccccggcagt gacgagcacc ggtcgttctc gatggcaacg acgccctcca cgcccggtgg
     1621 gatcgagttc ctcatcaaga agtacccggg cggcaagttc tccgggttgc tcgacgacgg
     1681 catcaccgcc ggcgacgaac tgtcgctcac cgggccgtat ggcacatcca ctttgaagga
     1741 gggtcacgtc ctgccggtgg tctgcgtcgc cggcggcgcg gggatggcgc cgatcctgtc
     1801 gattctgcgc cacttgggcg agacgggcag ccgacgcccg gttcgcttct actacggcgc
     1861 ccgtaccgcc tcggatctgt tctacctcga cgagatcgcc gaaatcggca gaggtctggt
     1921 cgatttcgtc ttcaccgcat gcctatccga gtcgacagag ggtgcgcttc ccgacacgct
     1981 gaccgtcgag gagggcaacg tgaccgacgt ggtcgcgcgc cacgaggccg agatcggcaa
     2041 aacggaggtc tacatgtgtg gaccaccgcc gatggtggac gccgctttgg atctgcttca
     2101 aacccatgcg gtgcccaagg accagatctt ctacgacaag ttcaccagcc ccatcttcga
     2161 ctgacttcaa atctcctaca gcccagagga aaacaggaag gaaaccaatg tctgcacccg
     2221 caaagcagcg cagctttccc aagatcgagt tcaccgactc ggaggcgggc gcgctggagt
     2281 tcccgagttc caagagccgc aagttcacct actacaagcc ggcgaagctg cgctcgacca
     2341 tgtacgaaga cgtcacgatc gacgtccagc ccgatccgga acgccatctt tcgcagggct
     2401 ggatctacgg cttcggcgac gggcccggcg gctacccgca ggactggacc gttgccaaat
     2461 cgtcgaactg gcacgccttc ctcgacccca acgaggaatg ggagcaaacg atctaccgca
     2521 acaacgcggc ggtggttcgc caggtggagc tgtgcctgcg gaacgccaag cgcgcccgtg
     2581 cctacgacgg ctggaactcg tcatggctga agttcctcga gtgcaatctc ggtgcgtgga
     2641 tgcatgtcga gaacggcttg gcgctgcatg tattcacctc cattcagcgg tcaggcccga
     2701 ccaacatgat caacaccgcc gtcgccgtga acgccgccca caagctgcgg ttcgctcaag
     2761 acttggcgtt gttcaatctc gacctgtccg aggcggcggg acctcccaag ggattcgacg
     2821 gcagtgcgca caaggaggtc tggcagtccg ctcccgagtg gcaactcacc cgcgaggtca
     2881 ccgagcgtct gaccgccgtc ggcgactggt gtgagctgct gttcgccacc aacatcgtgt
     2941 tcgaacagct ggtcggccag ctgttccgca gcgagttggt catgcagatc gctgcgcgca
     3001 acggcgacta catcacgccg acgattgtcg gcaccggtga gaacgactac gaccgcgatc
     3061 tggcctacac acgcaacctg ttccggatcc tgacccgtga cgagcagttt ggcgaatcaa
     3121 acaaggaact gttctcgtca tggctgtcga tatgggtgcc acggtgcctg gccgccgcgc
     3181 aggcgctgca gccgctgtgg tcgcagcccg ccgaaaaggt cgtcacgttc gcaaccagcc
     3241 tcggccacgt cgagagccag ttcgcgggcc tgctcgagtc gatcgacctc agtgccccag
     3301 aggcaaccat caaggaggat gacaagtgac catgcagttc ggatcagcca ccgaagtctc
     3361 caacatgtgc ggcgtgacgt tgatgaacac cccggtcggg cgtgtcgtcg ccgacgtgat
     3421 gggcgccaag gacggtgtgg agctcaccga atacccgtcg atgatccgcg tcgacggaca
     3481 gcgggttctc gacttcgact acgccgaact caccgaggcg ctcggcgagg aattcgacgg
     3541 ttcgatcttc gaggagatca gttccaccca ctacgggcgg atggtgcatc tcgacgaccg
     3601 caccctgctc ttcgccaatc ccgaggacgc ggccgagtac atcggcttcg acctcaccgc
     3661 gaagtaaccc gactagcaag gattttcgat gtacaccaaa gacggcaact cgtatttcat
     3721 cgtcgacggc cacgtccata tctgggacgg tcgcgcggcc aatcaccgca atatccacgg
     3781 caagcagttc atcgactgct tctacgacta tcaccgcaac ctcagccccg aggacgtggt
     3841 gtgggactac gacacctaca cctactacgg cggtgagcgg ctgatgcgcg acctgttcgc
     3901 cgaaggctat gtcgaccacg cgatcttcca ggccaccctg ctgagtgact tctacgtcaa
     3961 cggtttcggg cagaccgagg aggcattcgc gctgacacgg cagcaccccg acaagttgac
     4021 gtacaaccac gcctacgacc cgcgcaacgg cgaggccggc ctggtccagt tgcgccgcga
     4081 taccgagcga atgaagctgc agggcgtcaa gctctacacc gccgaatggc acggtgactc
     4141 acgcggttac aagctcgacg acccgtggtc acggcgctac ctcgaagagt gcatcgcgtt
     4201 gggcatccgc aacattcacg tgcacaaggg cccgacgatc cggcccctgg accgcgacgc
     4261 cttcgacgtc gccgacatcg acaaggtcgc gaccgattac ctggagctca acttcatcgt
     4321 cgagcacgtc ggattgccgc ggctggagga cttctgctgg atcgccacgc aggagtccaa
     4381 cgtctacggc ggcctggcgg tggcgatccc gttcatccac acccgtccac gctatttcgc
     4441 gcagatcatc ggtgagctgt tgtactggat cggcgacgac aagatcctct tcgccagcga
     4501 ctacgcgctg tggacgccga agtggctggt ggaaaagttt gtcgacttcc agatccccga
     4561 ggacatggcg gaatacgccc cgatcaccgt cgagcagaag aagaaaatcc tcggcctcaa
     4621 cgctgcggcg ctctacgaca tcaatgtgcc caccgagctt cagctggccg aggcggccgg
     4681 gcagcgcggt gtcgaggtcg cggccggtgc aaaggaggcg gtgccgctat gaccgtcgcc
     4741 acggtgtcca tcgagtccga aatccttgcg gcgctcgaca ccgtcaccga cccggaattg
     4801 gaccagccca tcaccggact gggcttcgtg cgctcggtga gcatcgatga cgacggcgtg
     4861 accgtgcacc tgcggctacc cacggcgttc tgttcaccga acttcgctta tctgatggcc
     4921 gccgatgccc aagacgcgct gcgcacagtc gacggcatcg gcgaggttcg ggtgttgctc
     4981 gacgatcatc atgacagcga caagatcaac gccgggttgg ccgccgaggc aggctatgtc
     5041 ggcacatttg gtgccgaggc cgaacagagc ctcgaccagc tgcgccggac cttcctgagc
     5101 aaggcccata ttgcggcgat ggagcggtgc gtcaccgcgc tgatgcgaca gaacgcactg
     5161 cacccgaacg agatacaccg gctcaccctg cgtgacctgc ccgatggtcg ggagaagacg
     5221 gcgctcctgc gccgccggtt cgccctcggc cttagcgtct gcccgaacgg gcgcatcgtg
     5281 gtcgacgacg caggccggac gctggcgtcc gaggcggtac cgatgcggtt gcggttcgcc
     5341 cgatccgtgc ggatctccat ggagggcaac gcccacttct gccgggggct gctcgccact
     5401 cggtacgacg acggcggaac tgccacaccg caattcacca acaccagaac gaggagccca
     5461 cgatgagggc cgtacaggtc gtcggatacc acaccaaact acagctcatc gacatccccg
     5521 aacccacgat cgaaaaaccc ctggacgtga tcgtacgcat cggtggcgcc ggtgtgtgcc
     5581 gcaccgatct gcacatcctc gaaggtcagt gggaagccaa gtccggcgtc gaattgccat
     5641 acaccatcgg tcacgagaac gccggctggg tacacgcgat cggcgacgcg gtcaccaacg
     5701 tcaaagtcgg cgacaaggtg atcctgcacc cgctgatcac ctgtgggctg tgccgggcgt
     5761 gccggttcgg cgatgacgtg cactgcgaga atagtcaatt ccccggcatc gacaccaacg
     5821 gcggctacgc ggaatacctg cgaaccaccg cgcgcagtgt ggtgaagatc gacgacgtac
     5881 tcgagcccgc cgacgtcgcc gcgctcgccg atgccggcct taccgcttat cacgccgcgg
     5941 ccaaggttgc gtgcgtcacc aggccgggaa atgtctgtgt ggtgatcggt gccggcgggc
     6001 ttggccacat cggaatccaa gtgctgaagg cgatctcggg tgtgacggtc gtcgtcgtcg
     6061 accgcaatcc cgacgccgtc aaactggcca aggacatcgg cgccgaccag ggcatcgtcg
     6121 cggacggcag ccatgtgcag cagatcctcg acctcaccgg aggccacggt gccgaggccg
     6181 tcctcgactt cgttggcgaa ggaggagcga ccaaagaggg cgtggccatg ctgcgccgcg
     6241 ccgggaacta cttcgtcgtc ggctacggag agaacatcga cgttcccact atcgacatca
     6301 tctccaccga gatcaacttc atcggcaacc tggtcggctc gtacaacgat ctccaggagc
     6361 tcatggtgct ggcggcgcag ggcaaggtca agctgcacac cagcgagtac ccgctcgacg
     6421 aattccagcg cgccctagac gatctcgatg cgggccgggt gcgcggccgc gccatcctgg
     6481 tcccctgacc cagaaaggag caagaccatg gcaaaggagc tgcggtacaa caccgaggcg
     6541 cggacacgtt tggagtacgg cgtcaacgcg ctcgccgatg cagtcaaggt gacgctgggg
     6601 ccgaagggac gcaacgccgt tctggagaag ctgaccggac ctccgaccat caccaacgac
     6661 ggtgtcacga tcgcccgcga gatccaactg tccgacccct tcgcgaacat gggcgcacag
     6721 ttggtcaagg aagtcgcgat gaagaccaac ggggtggtcg gtgacggaac caccaccgcc
     6781 acggtgctcg cccaggcgat ggttcgggaa gggttgcgtg ctgtcgaggc cggagccaat
     6841 cctatgcggg tgcgccgcgg catcgaacgc gccgtgcccg tcgttctcca attcctgaca
     6901 gagcacgcaa atccggtcaa cgggcggaac gacctgttgc acatcgccac actggccgcc
     6961 agtgacgacg aagtgatcgg tgaggtcatt acccgcgcgg tcgagcacgt cggtgaaatc
     7021 ggggtgatca ccaccgaaga gagcgatgcc cttggcctga ccgtggacgt cgtggacggc
     7081 atcgaattcg accacggcta catctcggcc tacatggtca ccaaccccga gcgcatggag
     7141 gccgcgctcg acaatccgct gatcctgttg accaacaaga agatcaccgc ggtccaggag
     7201 atcatgccga ccatcgaggt ggccaagcgg gcggaccgcc cgctgatggt gctcgccgag
     7261 gacgtggacg gtccggcgct gcagctactc gttggcggga acatgcacaa gaccatgctg
     7321 tcagtggtcg tgcgtgcacc cggcttcggt catcgtcgcg tcgccgaact ggaagacctc
     7381 gcggtcgcgc tgggtggtca cgtgatcgcc aaggacaccg gcgtcgaact gtccgaggtg
     7441 acactgcagc accttgggtc ctgcgaccga atcacggtga cggaacacga aacgacgatc
     7501 gtcggggcac acggcgagca acgactcatc gacgcgcgag tgggtcaact cgaggcgcag
     7561 cgtgagcggg ccaggatcga cgctgaccgc gacagcctcg acctgcgcat cgcccggctg
     7621 accggccggg tcgcggtgat ccgcgtcggc ggcgccacca gcgtggagct caaggagcgc
     7681 atgcttcgcg tcgacgacgc gctggccgcg acacgggccg cggtggaggc gggcatcgtc
     7741 tcgggcggtg gtacggcgct ggctcaagcg caccgcgcac tggcggatct cactctgcct
     7801 ggcgacgatg ccatcgg
//
LOCUS       DASRJA010000741         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1409596,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000741 DASRJA010000000
VERSION     DASRJA010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409596"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFC01_33565"
     CDS             <1..321
                     /locus_tag="VFC01_33565"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZN84586.1"
                     /translation="AMFLFVGGLKLTGAPDLVALFDAIGLGQWLRYVTGSIEVVSAVA
                     LLVPSWAAFGALLLIPTMVCAVVTHLFVVGGSAVPATVLLVGSLAIAWVRRDQLASVL
                     SRRS"
     gene            657..1673
                     /locus_tag="VFC01_33570"
     CDS             657..1673
                     /locus_tag="VFC01_33570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZN84587.1"
                     /translation="MASYRAVEVPEPGKLQIVERPVTEPAVGQVRIRVEACGVCHTDA
                     ATVSATYPGLTLPRVPGHEVVGRVDAVGGGVSRWRVGDRVGVGFFGGDDGVCEPCRRG
                     DFVNCQNPVIPGASIDGGYAEMMIAESRGIVAIPPELTSVEAAPLLCAGITTYNALRN
                     AGLRGGDLVAVQGIGGLGHLGIQFARRMGFRTVALARGREKEKLAADLGAHVYIDTAV
                     DDAVALLQRMGGAKAILATGTSGGSMGPLVSGLAARGKFIVVGVPGDPIQLNAFPLVF
                     GGRSIYGALTGTPIDNEDTLAFSVLANIRPMIETVPLEQAAAAYARMMQGKARFRMVL
                     TMTT"
BASE COUNT          358 a          643 c          663 g          385 t
ORIGIN      
        1 gcgatgttcc tgttcgtcgg cggactgaag ctgaccggcg cgcccgactt ggtcgcgttg
       61 ttcgacgcaa tcggcctcgg gcagtggctc cgctacgtga ccgggtcgat cgaggtcgtc
      121 tcggcggtgg cgctcctcgt accgtcctgg gcggccttcg gagcgctcct gctcattccc
      181 acgatggtct gcgccgtcgt gacgcatctc ttcgtcgtcg gcgggtccgc ggtgcccgca
      241 accgtgctgc tcgtcggatc gctcgcgatc gcgtgggtac gacgtgacca gctggcgagc
      301 gtgttgtcgc ggcggagttg agcgcacctc ccgttcgaaa cagtcctttc ccttggagcc
      361 accgggtgcg cgacgtgcgt ctcgatcacg caagactgtc cgccacaacc caaaccacac
      421 aatgacctac ctgtatcaat cagttgcgcc gtagcgataa atccgtcggc gattgggaga
      481 tgtggaacaa acggccgccg cggcattgat ggcggcggcc ttgcctgaac caggagcaac
      541 aactgtgagc ccagcacgca atcgtcgatg aaggttcacg gggcacagcg aggaaaatca
      601 atgaatccga agctgttcgt aaatcatgca aggccgttca cggcggaccg cacgtgatgg
      661 ccagctacag agccgtcgaa gtgcccgaac ctggcaagtt gcagatcgtc gaacgcccgg
      721 tcaccgagcc cgcggttggc caggtccgca ttcgggtgga ggcatgcggc gtctgccaca
      781 ccgatgccgc gacagtcagc gccacatatc cggggctaac gcttccgcgt gtgcctggcc
      841 atgaggttgt gggccgcgtc gacgctgtcg gcggcggcgt ctccaggtgg agagtcggcg
      901 atcgagttgg tgtcggcttc ttcggcggcg acgacggtgt gtgcgaaccc tgcagacgcg
      961 gggactttgt gaactgtcag aacccggtga tacctggggc cagcatcgat gggggctatg
     1021 ccgagatgat gatcgctgaa tcgcgcggca tcgtggcgat cccgcccgag ctcacttccg
     1081 ttgaggcagc ccccctcctc tgcgcgggca tcacgaccta caacgcactt cgcaatgccg
     1141 gccttcgcgg tggggatctg gtcgcggtgc agggcattgg cggcctcggg cacctgggca
     1201 tccagttcgc ccgccgcatg ggattccgta cggtcgcact cgcgcgggga cgcgagaagg
     1261 agaagctggc ggcggacctc ggtgcccacg tctacatcga cacggctgtc gacgatgccg
     1321 tggccctgct ccagcggatg ggcggtgcca aagcgatcct cgcaacgggt accagtggtg
     1381 gctcaatggg tccactggtg tcgggtcttg cggcccgcgg gaagttcatc gtggtgggag
     1441 ttccggggga cccgattcag ctgaatgcgt ttccgctcgt ctttggtggt cgctcgatct
     1501 acggcgcact gaccggtaca cccatcgaca acgaggacac ccttgccttc agcgtgctcg
     1561 cgaatatccg tccgatgatc gagaccgttc ctctcgagca ggcggcggcc gcctacgccc
     1621 gcatgatgca aggcaaagca cggttccgca tggtgctcac catgactacg tgaagtgacg
     1681 gccgggacat cacgagagag ccttcatggc caatcgacag gatcttcaga acgacgcgga
     1741 tgcaccccgg cttgattccg tcgtcacacg tgttcgcggt gaatattccg agatgccagg
     1801 gctccaactg acgtgcgcgc aggcgtgtcg tctgtggcac atgcatgttt ctacatgcga
     1861 gaagctgctc gaacagctcg ttcaggaggg cttcctctgc aagacggaca agggcgcgta
     1921 catcgccgcg ccgaccattc gtagacgaac ctagcaagac caagagggct gttcggcccg
     1981 cattgcttcc aagcgtgctc cttcacccta gggcacgcgg cgccgcatcc cgtccagaac
     2041 cagatcctg
//
LOCUS       DASRJA010000742         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_238630,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000742 DASRJA010000000
VERSION     DASRJA010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238630"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..983
                     /locus_tag="VFC01_33575"
     CDS             30..983
                     /locus_tag="VFC01_33575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN84588.1"
                     /translation="MTAPRITTPFGFSSTAAEVVAGVDLSGKRAIVTGASSGIGVETA
                     RALAGAGAAVTLAVRNAEAGERVAGEIRHSTGNDAVTVGTLELSDLTSVRTFAAAWSG
                     PLDILVNNAGVMAIQELTISTSGHEMQFATNHLGHFALALWLHDSLAAADSARIVSVS
                     SGGHLRSPVVFDDIDYAFRDYDSFGAYGQSKTANVLFAVEATRRWASDGIVANALMPG
                     GIATPLQRHLPEHYAAQALEAFRAAGTDFKTVEQGAATSVLLAASPLVEGVGGRYFEN
                     CNEAVRVDRRGGPGQGGVAPYALDPANAERLWELSLQMIDA"
     gene            976..1737
                     /gene="nei2"
                     /locus_tag="VFC01_33580"
     CDS             976..1737
                     /gene="nei2"
                     /locus_tag="VFC01_33580"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII Nei2"
                     /protein_id="HZN84589.1"
                     /translation="MPEGDTVWHTATILRDALAGKTLTRCDVRVPKFATVDLTGQVVD
                     EVISRGKHLFIRVGQASIHSHLKMEGSWKVNPVSKPSRAGYRIRIILEAGDIQAAGID
                     LGVLEVLKRDHDLDVVSHLGPDLLGDDWDPHVAAVNLVADPDRPLSESLLDQRVLAGV
                     GNVYCNELCFVTGHLPTTPVSAVEDPLRMVQRARDMLWLNRSRWNRTTTGDTRPGRDV
                     WVYGRSGKPCRRCGTPIQSGSSGDRVSYWCPVCQT"
     gene            complement(1851..>2094)
                     /locus_tag="VFC01_33585"
     CDS             complement(1851..>2094)
                     /locus_tag="VFC01_33585"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZN84590.1"
                     /translation="LGSANLTGGKLFHILSGNLSFQIEHHLFPDIPAHRHAEISQEVR
                     EICGRYGVPYNTGPLPKQFATVVRKIVKLALPDRRR"
BASE COUNT          358 a          649 c          710 g          377 t
ORIGIN      
        1 gcgggtcggg agtaccgttt gactcgctta tgactgcacc ccggatcacc acaccgtttg
       61 gattcagctc gacggccgcg gaggtcgttg ccggcgttga tctgtccggt aaacgggcca
      121 tcgtcaccgg cgcgtcgtcg ggtatcggcg tcgaaaccgc tcgggcactg gccggcgccg
      181 gtgcggccgt cacgcttgcc gtgcgcaacg ccgaggcggg tgaacgagtg gcgggcgaga
      241 ttcgccacag caccggcaac gacgccgtca ccgtcggcac actcgagctg tcagacctga
      301 ccagcgtccg gaccttcgcc gcagcctgga gcggaccgct tgacatcctg gtcaacaacg
      361 caggtgtgat ggccatccaa gagctgacga tatcgactag cggccacgaa atgcagttcg
      421 ccacaaatca tctcgggcat tttgcgctag cgctctggct gcacgattcg cttgccgcgg
      481 cggactcggc acgcatcgtg tcagtgagct ccggcgggca tctgcgctcg ccggtggtgt
      541 tcgacgacat cgactatgcg ttccgcgatt acgactcgtt cggtgcttac ggtcagtcga
      601 agaccgccaa tgtgctgttc gccgttgagg ccacgcgacg gtgggcgtcc gacggcatcg
      661 tcgccaacgc gctgatgccg ggtgggatcg cgacgccact gcagcggcat ctgcccgagc
      721 actatgcggc gcaggcgctc gaggcgttcc gggcggccgg cacggacttc aagacggttg
      781 aacaaggtgc ggcgacatcg gtgttgctgg ccgcttcccc actagtggag ggggttggcg
      841 ggcgctattt cgagaactgc aacgaggccg tgcgggtaga ccggcgcggc gggccgggac
      901 aaggcggggt tgcgccgtac gcgctcgacc cggccaacgc cgaacggctg tgggaactct
      961 cgctgcagat gatcgatgcc tgagggtgat accgtctggc acaccgccac catcctgcgc
     1021 gatgcgctgg ccggaaagac gctgacccgt tgcgacgtgc gtgtgccgaa attcgccacc
     1081 gtcgacctta cgggtcaggt cgtcgacgag gtaatcagtc gcggcaagca cctgttcatt
     1141 cgcgtcgggc aggccagcat ccattcgcat ctgaagatgg aaggaagctg gaaggtcaac
     1201 cccgtcagca agccgagcag ggcgggatat cgcatccgga tcatcttgga ggcgggcgac
     1261 attcaggcgg caggcattga tctgggcgtg ctcgaagtgt tgaagcgcga ccatgatctg
     1321 gacgtcgtct cgcatctcgg gcccgacctt cttggggacg actgggatcc acatgtcgcg
     1381 gcggtgaact tggtcgccga tccagaccgc ccgctgtcgg agtcattgct cgaccagcgg
     1441 gtgttggccg gcgtcggcaa cgtctactgc aacgaattgt gcttcgtcac cggccacctg
     1501 cccaccactc cggtcagtgc cgtcgaagac ccgctgcgga tggtgcagcg cgcccgggac
     1561 atgttgtggc tgaaccgctc tcggtggaat cgcaccacaa cgggtgacac ccgccccggc
     1621 cgagacgtat gggtatacgg ccggagcggt aagccgtgcc gccggtgcgg gacgccgatc
     1681 caatcaggta gcagcgggga ccgcgtctcc tactggtgcc cggtctgcca gacctgacag
     1741 acataggttt cccatcgccg agttcgacat gagggtcgtg aaataggccg gttggccgga
     1801 aaccacgacc gtcatgtcga tttcgcggct atcttggcga cggtgtatcc ctaccggcgc
     1861 cggtccggca gcgccagctt gacgatcttg cgcacgaccg tggcgaactg tttcggcagc
     1921 ggacccgtgt tgtacggcac cccgtagcgg ccgcagatct cgcgcacctc ttgagagatc
     1981 tcggcgtgcc ggtgcgcggg gatgtcgggg aacaggtgat gctcgatctg gaacgacagg
     2041 ttgccgctca ggatgtgaaa gagcttgccg ccggtcaggt ttgccgatcc gagg
//
LOCUS       DASRJA010000743         6523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2364904,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000743 DASRJA010000000
VERSION     DASRJA010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2364904"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VFC01_33590"
     CDS             complement(<1..1012)
                     /locus_tag="VFC01_33590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HZN84591.1"
                     /translation="MSPGGSQGLGPGDSDFVVVANRLPIDMERLPDGSTTWKRSPGGL
                     VTALEPLLRRRRGAWIGWPGIPDADDEPIEQDDMLLCPVRLSADDVANYYEGFSNATL
                     WPLYHDVIVKPIYHREWWDAYVDVNRRFAEATAKVAAQGATVWVQDYQLQLVPKMLRM
                     LRPDLIIGFFLHIPFPPVELFMQMPWRTEITEGLLGADLVGFHLPGGAQNFMILSRRL
                     VGANTSRATVGVRSRFGEVNVGFRTVKVGAFPISIDSTALDEQSRTRAIRQRAKEIRN
                     ELGNPRKILLGVDRLDYTKGIDVRLRAFSELLAEDRAKRDDTVLVQLATPSRERVESY
                     IEM"
     gene            complement(1013..1174)
                     /locus_tag="VFC01_33595"
     CDS             complement(1013..1174)
                     /locus_tag="VFC01_33595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84592.1"
                     /translation="MPAKSDPAELGDVEPLADSTARQARRVVAAYANDADECRIFLSM
                     LGIGPKLEA"
     gene            complement(1221..1571)
                     /locus_tag="VFC01_33600"
     CDS             complement(1221..1571)
                     /locus_tag="VFC01_33600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intersectin-EH binding protein Ibp1"
                     /protein_id="HZN84593.1"
                     /translation="MATLELSVRRLILAGGFAVAVAAAPAIAVFTVPSASVPLAQCPA
                     GEEEDTFTGVCVPHTVPNSGAVFSSIPGNPSLPAIDGIPCTGHNSGQCIGLAEEAEAM
                     GPQPVPRSTVSSSP"
     gene            complement(1699..2049)
                     /locus_tag="VFC01_33605"
     CDS             complement(1699..2049)
                     /locus_tag="VFC01_33605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intersectin-EH binding protein Ibp1"
                     /protein_id="HZN84594.1"
                     /translation="MTTPPIPARRLILAGGFALAIAIAPGAAILAHPTHSAPLAQCSS
                     GEEEDVFTTSCTPFLVPNSPQGFTSTAANPDIPEIQGIPCTGRDSGACIGLSEDEAAA
                     GPQPIPRSTISASP"
     gene            complement(2209..2553)
                     /locus_tag="VFC01_33610"
     CDS             complement(2209..2553)
                     /locus_tag="VFC01_33610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intersectin-EH binding protein Ibp1"
                     /protein_id="HZN84595.1"
                     /translation="MATLKSPARRLILAGGFAVAIAAAPAVAVVALSTDSPTIAACPG
                     GESEDTFTGACVPDLVPNSPAGNFTSPGGLPEVDNIPCTGANSGQCIGLSEEEQAQGP
                     MPVPRSTVSSSP"
     gene            2867..3802
                     /locus_tag="VFC01_33615"
     CDS             2867..3802
                     /locus_tag="VFC01_33615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZN84596.1"
                     /translation="MASPDLTSAEPVGGEAPQQVTYETLDEGRIARIWLNRPDAQNAQ
                     SRTLLVQLDEAFGRAEADDAVRVVILAARGKNFSAGHDLGSEQALLERKPGPAQHPTF
                     RSHGATVPAIAERTYLQEWHYFFENTRRWRDLRKITIASVQGNAISAGLMLIWACDLI
                     VAADDAKFSDVVGVRMGMPGVEYYAHPWEFGSRKAKELLLTGDSIDADEAYRLGMVSK
                     VFPRAELEDKTVEFARRIAERPTMAALLIKDSVNAASDAMGFTEALRHAFHIHELGHA
                     HWAAHNENRYPVGLPPNVPDWRTLGAPKPARRDEP"
     gene            3847..4683
                     /locus_tag="VFC01_33620"
     CDS             3847..4683
                     /locus_tag="VFC01_33620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN84597.1"
                     /translation="MQLSLADRTILVTGGGSGIGKGVAAAVVAAGGNAMLVGRSADRL
                     AGATEEIESGGGAGSVRYEPADVTNEEEVARVVDAATAWNGRLHGVVHCAGGSETIGP
                     ITQIDSDAWRRTVDLNVNGTMYVLKHAARELVRGGGGSFIGISSIAASNTHRWFGAYG
                     VSKSAVDHLMQLAADELGPSWVRVNCIRPGLIRTDLVTAVFDSPEVSGDYAASTPLPR
                     PGEVEDIASAAVFLLSDASSYITGQVINVDGGQMLRRGPDFSGMLEPVFGADGLRGVV
                     SS"
     gene            4770..6341
                     /locus_tag="VFC01_33625"
     CDS             4770..6341
                     /locus_tag="VFC01_33625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HZN84598.1"
                     /translation="MFESLAATTDGASGADGVAAWARVENAACARRLAMTADMMEARR
                     SADGSAEREQWCLDNWDAVAAEVAAAQNVSLGMASHQLMVAWALREHLPRVAEVFAAG
                     QISYRMVNAIVYRTALIRDPQARAKVDTELAAAAVEWGSLSVAKVEAAIDYWVDRYDP
                     DAVRRVELSARGRHVDVVDANNGSGVACVEGQLFTDDAAALDKRLDAMARGVCDADPR
                     TIEQRRADALGALAHGGDRLACQCGAADCAAAETGPSTVVVNVIAEEKSMADDTAVQL
                     DGASPPGPTTAELREMTLAGALAQPPGTGPANTNPAVVMGGGMLPAPLLAAKLAGTAT
                     IRPVIHPGDSPPEPRYTPSAGLARFVRCRDLTCRFPGCDEPADHCDLDHTIPYPLGPT
                     CASNMGCLCRKHHLLKTFWGWVNRQLPDGTMIWTAPSVQTYTTYPGSRLLFPTLCRPT
                     APVSAPADGPSAAPNRGLMMPRRKTTRAQDRAKRIEAERARNREIRQDPEKSWDDAYF
                     PSRPPPPGEEDPPPF"
BASE COUNT         1133 a         2007 c         2230 g         1153 t
ORIGIN      
        1 gcatttcgat gtagctctcg acgcgctccc ggctgggcgt cgcgagctgc acaaggacgg
       61 tgtcgtcgcg cttggcccgg tcctcggcca gcagctccga aaatgcccgc agccgaacgt
      121 caatgccctt cgtgtagtcc agccggtcca cgccgagcaa aatcttgcgc ggattgccga
      181 gttcgttgcg aatctccttg gcccgctgcc gaatcgcgcg ggtgcgtgac tgctcgtcga
      241 gcgccgtgga atcgatggaa atcgggaacg cgccgacctt gacggtgcgg aagccgacgt
      301 tgacttcgcc gaaccgggac cgaacgccga ccgtcgcgcg tgaggtgttc gcaccaacga
      361 ggcgtcgcga caggatcatg aagttctgtg cgccgccggg caaatggaac ccgaccagat
      421 cggcgccgag cagaccctcg gtgatctcag tgcgccacgg catctgcatg aacagctcca
      481 ccggcgggaa tggaatatgc agaaagaagc cgatgatcaa gtcgggccgc agcatccgca
      541 gcatcttcgg caccagctga agctgatagt cctgcaccca caccgtcgcg ccctgtgccg
      601 ccaccttcgc ggtggcctcg gcgaagcggc ggttgacgtc gacgtaggca tcccaccact
      661 cgcggtggta gatcggcttg acgatgacgt cgtggtacag cggccacagt gtcgcgttcg
      721 agaagccctc gtagtaattg gctacgtcgt cggccgacag ccggaccggg cacaggagca
      781 tgtcgtcctg ctcgatcggc tcgtcgtcgg catcgggtat ccccggccag ccgatccacg
      841 cgccgcggcg acggcgcagc agtggctcca gagcggtgac taggccgccg gggctccgct
      901 tccacgtggt gctgccgtcg ggcagccgtt ccatgtcgat tggtagccgg ttggccacca
      961 cgacgaagtc cgaatccccg gggcccaggc cttggctgcc ccccggagac acttacgcct
     1021 cgagcttcgg tccaatgccg agcatcgaca ggaagatgcg acactcgtca gcgtcgttcg
     1081 catacgctgc gacgacgcgc ctggcctggc gtgcagtgct gtcggctagc ggctcgacgt
     1141 cgccgagttc ggcggggtca gatttggcag gcatacgaca actctaggcg accgcgcggt
     1201 cacctgtgcc gtgccgcccc ctacgggctc gaactgacgg tcgaacgagg caccggctgg
     1261 ggccccatgg cctcggcttc ttccgccagg ccaatgcact ggcccgagtt gtggccggtg
     1321 cagggaatgc catcgatcgc gggcaggctc gggttgccgg gaatgctgct gaacaccgca
     1381 cccgagttcg gcacggtgtg ggggacgcaa acgcccgtga aggtgtcctc ttcttcgccg
     1441 gccgggcact gggccagcgg caccgaggcg ctgggaacgg tgaagacggc gatcgccggg
     1501 gctgcggcaa ccgcgaccgc aaaaccgccg gcaaggatca gtcgtcgtac tgaaagctca
     1561 agggtcgcca tcgtcacgcc ttctgtctgt agttattgac catctcgtcg ggagaattct
     1621 atggaagctg aaagaaatct gcatacgttc cacgagttcc ctgtgaatgc gccccttgag
     1681 cttgcggatt agcccccgtt atggactggc gctgatggtc gagcgcggta tcggctgcgg
     1741 gccggcggcc gcctcatctt cggaaagtcc gatgcaggcg cccgaatctc ggccggtgca
     1801 cggaatgcct tggatttccg gaatgtcggg gttggccgcc gtcgacgtga atccctgtgg
     1861 cgagttcggc accaggaaag gcgtgcagga agtcgtgaag acgtcctctt cctcgccgga
     1921 actgcattgg gcaagcggcg cggaatgcgt tggatgcgcg aggattgcgg cgccgggcgc
     1981 aatagcgatc gcgagggcga atccgccggc gaggattagg cgccgggctg ggatgggggg
     2041 tgtcgtcatc gatttgcctt ctgttgtcgg gtgtttctcg ttgcctggat tgtacgaacg
     2101 ctgaaagcgg actgcaggca ttcttcgtgt tagctgggaa tgtgtctgcg gcgcatacga
     2161 aaaagctggg gcgcactgct gttgcgcccc agctttgtcg ctatcggatt acgggctcga
     2221 gctgaccgtc gagcgtggca ccggcatcgg cccttgggct tgctcttcct ccgacaagcc
     2281 gatgcattgg ccggaattgg ctccggtgca cgggatgttg tcgacctcgg gcagcccgcc
     2341 gggcgaggtg aagttacctg caggcgaatt cggcaccaga tcgggcacgc acgcaccggt
     2401 gaaggtgtcc tcggattcac cgcccggaca tgccgcgatc gtcggcgaat cagtagatag
     2461 ggcgacgacg gcgaccgccg gcgcggccgc aattgcgacc gcgaacccgc cggcaaggat
     2521 cagtcgtcgt gctggagact tcaaggtcgc catcatcacg ctttctgttg ttgtttgctg
     2581 accgttgtga gcgggaagag gtatcgacgc tgccagccag ctggatgcgc tctacgaatt
     2641 ccctgccgat ggctcgtccg aagttgtacc ggcagaggcc atctgctatc gcaaacatta
     2701 cgctcagtcg ctgaaaaacg tcgcctcatc gtcaataaaa tggtgcggat gtcccatttc
     2761 tgtgagcttg ctgtcaggtt gctgtggcac cgcttcacgg gtgcttgccg tggcaccgta
     2821 atgtgcaatc catcgatagt ttttcaagcg aacgaggtgg tccgctgtgg caagccctga
     2881 tctgaccagt gccgaaccag tcggaggcga agcgccccag caggtgacgt acgagactct
     2941 cgacgagggg cgtatcgcac ggatctggct caaccggcct gacgcccaga acgcccagtc
     3001 ccgaacgctg ttggtgcagc tcgacgaggc cttcggccgt gccgaggctg acgatgcggt
     3061 ccgtgtggtg attcttgcgg cgcgcggcaa gaacttctcg gccggacacg atcttggttc
     3121 ggaacaagcg ctgctcgaac gcaaacccgg tcccgcgcag catccgacat tccgttcaca
     3181 cggtgccacc gtgcctgcga tcgcagagag aacgtatctg caggagtggc actacttctt
     3241 cgagaacacc cgccgctggc gggatttgcg aaagatcaca atcgcgtcgg ttcagggcaa
     3301 tgcgatctcc gcggggctga tgctgatctg ggcctgcgac ctcatcgtgg ccgccgatga
     3361 cgcgaaattc agcgacgtcg tcggcgtgcg gatgggcatg ccaggtgtcg aatactatgc
     3421 gcatccatgg gaattcggtt cacgcaaggc caaagagctt ctgctgaccg gtgattcgat
     3481 cgacgccgat gaagcttacc gcctcggcat ggtgtcgaag gttttccctc gcgcggaact
     3541 cgaagacaag acagttgaat tcgcgcgccg catcgccgag cgtccgacca tggcggcgct
     3601 gctcatcaag gattcggtaa atgcggccag cgacgcgatg ggattcactg aggcattgcg
     3661 gcacgccttc cacattcatg agctcggcca tgcgcactgg gcggcgcata acgagaaccg
     3721 ctacccggtc gggctgccgc caaacgtgcc ggactggcgc acgctcggag cgccgaagcc
     3781 tgcgcgccgc gacgagccgt gacgtataac tagaacctgt tctagtcaca ggacgaaggg
     3841 gtcgcggtgc agctctcgtt ggcagatcga acaatcctgg tcactggcgg tggaagcgga
     3901 attggcaagg gcgtcgcggc tgccgtggtc gctgcgggcg gcaatgccat gctcgtcggt
     3961 cgcagcgccg accggctcgc tggcgccaca gaggagatcg agtccggcgg tggtgccggc
     4021 tcggtgcgct acgaaccggc cgacgtcacc aacgaggagg aagtggctcg cgtcgtcgat
     4081 gcggccacgg catggaatgg ccgactgcat ggcgtcgtgc attgtgccgg cggcagcgag
     4141 acgatcggac cgatcacgca gatcgactcc gacgcctggc ggcgcaccgt cgacctgaat
     4201 gtgaacggca ccatgtacgt cctcaagcac gcggcccggg aactggtcag gggtggcggc
     4261 gggtcgttca tcggtatttc gtcaatcgcg gccagtaaca cacatcgatg gttcggcgcg
     4321 tacggcgtct cgaagtcggc ggtcgatcac ctgatgcagt tggcggccga cgaattgggc
     4381 ccgtcgtggg tgcgggtgaa ctgcatccgt cccggcctga tccgcacgga tctggtgacg
     4441 gcggttttcg actctcccga ggtcagcggt gactatgcgg ccagcacgcc actgccgcgg
     4501 ccgggcgagg tggaggacat cgctagtgcg gcggtgttcc tgctgagcga tgcgtccagc
     4561 tacatcaccg gccaggtgat caacgtggac ggcggccaga tgttgcgccg gggtcccgac
     4621 ttctcgggga tgctcgaacc ggttttcggc gccgatgggc ttcgcggggt cgtatccagc
     4681 taggcgaagt tatccccagt cgcgaattca tccacaggtt gggttggtga gctggcgttt
     4741 agtcggtttg gctgaatatt gtcgaaagta tgttcgaatc gctcgcagcg accaccgacg
     4801 gcgcatctgg cgctgacggc gttgcagcgt gggcgcgcgt cgagaacgcc gcatgcgctc
     4861 ggcggttggc gatgacggcg gacatgatgg aagcgcggcg gtccgcggat gggtcggccg
     4921 agcgtgagca gtggtgcctg gacaattggg acgcggtggc cgccgaggtg gccgcggcgc
     4981 agaacgtgtc gttaggcatg gcctcgcatc agctgatggt cgcctgggcg ttgcgcgaac
     5041 acctgccgcg ggtggccgag gtgttcgccg ccgggcagat ttcctaccgg atggtgaatg
     5101 cgatcgtgta ccgcaccgcg ttgatccgcg acccgcaggc gcgcgcgaag gtcgacaccg
     5161 agttggccgc ggccgcggtg gagtgggggt cgctgtcggt ggccaaggtc gaggcggcca
     5221 ttgattactg ggtggatcgc tacgatccgg atgcggtgcg ccgcgtcgag ctcagcgcgc
     5281 gaggccgaca tgtcgatgtg gtcgatgcga acaacggcag cggcgtggcc tgtgtcgagg
     5341 ggcagctgtt caccgatgac gcggcggcgt tggataagcg cctagatgcg atggcgcgcg
     5401 gggtgtgcga tgccgatccg cgcacaatcg agcagcgccg cgccgatgcg ttgggggcgt
     5461 tggcccacgg tggggatcgg ttggcctgcc agtgcggggc cgccgattgt gcggccgcag
     5521 agacagggcc cagcaccgtg gtggtgaatg tgatcgccga ggaaaagagc atggccgatg
     5581 acaccgccgt gcagctggac ggggccagcc cgcccggacc gaccaccgcc gagctacggg
     5641 agatgaccct cgccggggcg ctggcccagc cacccggaac ggggccggcg aatacgaatc
     5701 cggcggtggt gatgggcggc ggcatgctgc ccgcaccctt gctggcggcc aagttggcgg
     5761 gcaccgcgac gatccggccg gtgattcatc ccggcgacag cccgcccgag ccgcggtaca
     5821 ccccgtcggc gggattggcc cggtttgtcc gatgccggga tctgacgtgt cgcttcccgg
     5881 ggtgtgacga acccgccgac cattgcgatt tggaccatac gatcccgtat ccgctggggc
     5941 cgacctgcgc atccaatatg ggctgcctat gccgaaaaca ccacctgctc aagacattct
     6001 ggggttgggt gaatcgacag ctacccgacg gcaccatgat ctggaccgcg cccagcgtgc
     6061 agacctacac gacttatccg ggtagccggc tgctgtttcc gacgctgtgc cggcccaccg
     6121 cgccggtcag cgcgccggcc gacggaccca gcgcggcgcc caaccgtggc ctgatgatgc
     6181 cgcgccgcaa aaccacccgg gcccaagatc gcgccaaacg catcgaagcc gaacgggctc
     6241 gaaaccgtga aatccgtcag gaccccgaaa agtcctggga cgacgcctat ttcccatcac
     6301 gaccaccccc gccgggtgaa gaggacccac caccgttctg agcggggccg ttacgtgcgc
     6361 ttccgcagga ggcgctgagc cacgaccccg actgccacga cggcgcacaa cacgaccgcg
     6421 atgagctggg cttggagccc cacaaccgcg agaatgttga cggtatgcga gccggcgtcg
     6481 ctgacgcatt ggaagtgcga gccgcggtgc ttgatgttgt gcg
//
LOCUS       DASRJA010000744         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1680253,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000744 DASRJA010000000
VERSION     DASRJA010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680253"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..904
                     /locus_tag="VFC01_33630"
     CDS             254..904
                     /locus_tag="VFC01_33630"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN84599.1"
                     /translation="MKVHRWKTITALAAAASLVIAIGAAPSQAASLVESTKVSVPLSA
                     SDLSKLPLTDRHLTKREKANLAVVLRAYRVAEGDVLDAQGFIDLFAEDGVLNGIGGVS
                     GLDTLRSQQLGGLVVWMGTFLPDVHRQLKQVTVRGDVVSIELSIQGTFLGPFDTPAGV
                     IRPTGAKIDIPTADFWYLRNGKVEVFDCHIGFTTLYAQLGVLPDYASAVEASAPGR"
     gene            1032..>3088
                     /locus_tag="VFC01_33635"
     CDS             1032..>3088
                     /locus_tag="VFC01_33635"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HZN84600.1"
                     /translation="MVKSGGISRRALVALPALAALGVSDVAAAASPPGDVVGKITVGY
                     QGWFACIGDGAPINGWWHWAHNWGQPPSPGNNAIVSWPDMRDYARGYLTAFPNLGNGS
                     AASLFSQYDQQSVDTHFRCMRDYGCDTAALQRFNPFGGEGPTRDAMAAKVRQAAEAFG
                     RKFYIMYDVTNWTDMQSEIKTDWSTRMSAHTTSAAYARQNGKPVVCVWGFGFSDPGRP
                     FSPVACLDVINWFKAQNCYVIGGVPTYWRTGINDSRPGFIDVYHAFNMLSPWMVGRTG
                     DIAGLDWFYTNVNVPDQADCNARGIDYQPCVMPGDLSAHHRVHGDFMWRHFYNMVRVG
                     AQGIYISMFDEYNEGNQIAKTAENASMVPTGSGFVTLDEDGTACTSDYYLRLTADGGR
                     MLKGQIALTPTRPTVPWPGGNGQTNLARGRATAESSHSQVYVSGNAVDGNSASYWESA
                     NNAFPQWIQVDLGTALSVGRVVLKLPPTWGARTQTLSVTDGATGEVLVPSGARQFDPA
                     TGNTVTLTIPQTTRRYVRVTFIANTGWPAGQVSELEVYGAGGGDTQPPSVPGNLVATG
                     RTPTSVSLAWQASSDNVAVTGYDMRQNGIIVATVIGTSGTIGGLNPATTYNFTVTARD
                     AAGNVSAPSNTVSVTTDPAPGTNLARGKATAESSHTQVYVSGNAVDGNSASYWESANN
                     AFP"
BASE COUNT          580 a         1037 c          941 g          530 t
ORIGIN      
        1 ggaccaccga tgacggcccc gtcccgggca gcccgccccg ctaccccctc agccgacatg
       61 tcggtcatcg cccatcttct ccaggcaccc ggccagagta acccccgtag aacgccggca
      121 gaccacgggc ggatcacctg aatccagcga cgacgctcgc tgtcccagca ccgagcatct
      181 gatttgtaac gacacccggc gagagacccg ttgcgacgga tctcagatcc tgagaccaca
      241 ggaggacatg accatgaagg ttcatcgctg gaagacgatc accgccttgg ccgcggccgc
      301 gagcctggtt atcgcgatcg gcgccgcgcc gtcacaggct gcgtctctcg tcgaaagcac
      361 gaaggtcagc gtgccattga gcgcgagcga cctatccaag ctgccgctca ccgaccgcca
      421 cctgaccaag cgtgagaagg ccaacctcgc ggtcgtgctg cgcgcctacc gcgtggcgga
      481 gggtgatgtc ctggacgccc aagggttcat cgacctgttc gctgaggacg gtgtgctcaa
      541 cggcatcggc ggcgtgtctg gtctggatac cttgcggagc cagcaactgg gcggtttggt
      601 cgtctggatg ggcacgttcc ttcctgacgt tcaccggcag ctgaagcagg tcaccgtgcg
      661 cggcgacgtg gtcagcatcg agctgtcgat ccagggcacc ttcctcgggc cgttcgacac
      721 gcccgccggc gtgatccggc cgacgggggc caagatcgac atcccgaccg ccgacttctg
      781 gtacctgcgc aacggcaagg tcgaggtgtt cgactgccac atcgggttca ccaccttgta
      841 cgcccagctg ggcgtactgc cggactacgc atccgcagtc gaggcgtccg cgcccgggag
      901 gtaacaccaa cgcgagcttc cagttgcgcc ggcgggcgct gcgctcccgg cgctacgacc
      961 gcggagttcg aagtttgcgg acctttgtca aggtcttgcg gcagaagatt gacgtattgc
     1021 agtatatcgt catggttaag tcaggcggca tctcccgccg ggcgctggtc gccctcccgg
     1081 cccttgcggc ccttggcgtt tcggatgtag ctgcggcggc cagcccaccc ggcgacgtgg
     1141 tcggaaagat cactgtcggt taccaaggct ggttcgcttg catcggtgat ggggcgccga
     1201 tcaacgggtg gtggcattgg gcgcataact ggggccagcc gccgtcgcct ggtaacaacg
     1261 ccatcgtctc ctggccggac atgcgggatt acgcccgcgg ctaccttacg gcctttccca
     1321 acctcggaaa cggaagcgcc gcctcacttt tctcccagta cgaccagcag tccgtcgaca
     1381 cgcatttccg ctgcatgcgc gactacggct gcgacacggc ggcgctgcaa cgcttcaacc
     1441 ccttcggcgg ggaggggcca acccgcgatg ccatggccgc caaggtccgg caggccgccg
     1501 aggcgtttgg gcgcaagttc tacatcatgt acgacgtcac caactggacc gatatgcagt
     1561 cggagatcaa gaccgactgg tcgacgagga tgtcggcgca caccacctcc gcagcgtatg
     1621 cacggcagaa cggcaagccc gtagtttgcg tgtggggctt cggcttcagc gatcccggcc
     1681 gcccgttcag tcccgtagca tgcctcgacg tcatcaactg gttcaaggcg cagaactgtt
     1741 acgtcatagg cggggtcccg acctactggc gcaccggcat caacgattcg cgccccggat
     1801 tcatcgacgt gtaccacgct ttcaacatgc tctcgccctg gatggttggg cgcacaggcg
     1861 acatcgctgg gctggactgg ttctacacca acgtcaacgt gccggaccag gccgactgca
     1921 acgcccgtgg catcgactac cagccgtgtg tcatgcccgg cgacctatcg gcacaccaca
     1981 gggtgcacgg cgactttatg tggcgccact tctacaacat ggtgcgcgtt ggggcgcaag
     2041 gcatctacat ttccatgttc gacgagtaca acgagggcaa ccagatcgcc aagaccgccg
     2101 agaacgcctc gatggtcccc accggttcgg gcttcgtcac actcgacgaa gacggcacag
     2161 cctgcacatc cgactattac ctgcgcctga ccgccgatgg cggcaggatg ctcaagggcc
     2221 agatcgcttt gaccccaacg cgtccgacgg tcccgtggcc cggcggtaac ggccagacca
     2281 acctggcgcg cggcagggcg acggcggaga gtagccattc gcaggtttat gtctcgggca
     2341 acgctgtcga cgggaactcg gcatcgtact gggaaagtgc caacaacgca ttcccgcaat
     2401 ggattcaagt ggacctcggc accgcgctgt ccgtcggccg agtggtgctc aagctaccac
     2461 cgacgtgggg cgcccgcacg cagacgctgt ccgtgaccga cggcgcgacc ggagaagtcc
     2521 ttgttcccag cggtgcgcgt caattcgatc cggcgacggg caacacagtg acactgacaa
     2581 tcccgcagac cacccgacgg tatgtgcgtg tcactttcat cgccaacacc ggctggcccg
     2641 ccggccaggt ctccgaactg gaggtctacg gtgccggtgg tggcgacacc caaccaccct
     2701 ccgtcccagg caacctcgtc gccaccggga gaaccccgac gtccgtttcc cttgcctggc
     2761 aagcttcttc ggacaatgtc gcggtcactg ggtacgatat gcggcaaaat ggcatcatcg
     2821 tggcgactgt catcggtaca tcaggcacga tcggtgggct gaatcccgcc accacctaca
     2881 acttcacagt caccgctcga gatgcggcag gcaacgtgtc ggcgccatcc aacacagtgt
     2941 cagtcacgac cgatccggcg ccgggtacca atctcgccag aggcaaggcg acggcggaga
     3001 gtagccacac gcaggtttat gtctcaggaa acgctgtcga cgggaactcg gcatcgtact
     3061 gggaaagtgc caacaacgca ttcccgca
//
LOCUS       DASRJA010000745         9186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4058554,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000745 DASRJA010000000
VERSION     DASRJA010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4058554"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFC01_33640"
     CDS             complement(<1..426)
                     /locus_tag="VFC01_33640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN84601.1"
                     /translation="MANLINVERATVGYGTRTLLDEVSLGVEDGDAIGVVGRNGDGKT
                     TLLQVLTETRQPDSGRVTHTSGLSVGYLRQGDELLGATVRDVIVGGRPDHVWAAEPDT
                     RAVVSQLLSGVELDAEVSRLSGGERRRVALAEVLLGGHDV"
     gene            510..3476
                     /locus_tag="VFC01_33645"
     CDS             510..3476
                     /locus_tag="VFC01_33645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HZN84602.1"
                     /translation="MDLSAITRPVGRLVATAQNGLEVLRYGGLETGAVPSPFQMIQSV
                     AMYRLRRYFPPDARPGAKRPGPPVLMVHPMMMSADMWDVTRDDGAVGILHKAGIDPWV
                     IDFGSPDKVEGGMDRNLADHVVALSEAIDTVKEVTGRDVHLAGYSQGGMFAYQTAAYR
                     RSKDLASIVAFGSPVDTLAALPMNMPASFAPAAANFMADHVFSRIDIPGWLARTGFQM
                     LDPIKTAQSRLDFLRQLHDREALLPREQQRRFLASEGWIAWSGPAIAELLKQFIAHNR
                     MMSGGFSIHGDLVTLSDIDCPVLAVVGEVDDIGQPASVRGIKRAAPQADVYEFLIRAG
                     HFGLVVGSKASTQTWPAVAQWVKWIDGTGDMPHGVSPMALQPAEHAESGVSLSSRVAH
                     GTAAATEMAFSLARTAADAIVAANKSARTLAIETYRTLPRLARLGQINDHTRISLGRI
                     MSEQARDVPEGECLLFDGRVHTFEAVDRRINNVVRGLIEVGVRQGAHVGVLMETRPSA
                     LVAIAALSRLGAVAVLMPPDVDLAAVARLGGVSEIIADPSNLEAARRLDMRVLVLGGG
                     ESRDLHLPEHVDVVDMEKIDPDVVDLPGWYRPNPGLARDLAFIGFSAIGGELVARQIT
                     NYRWALSAFGTASAANLGRGDTVYCLTPLHHQSGLLVSLGGAVVGGSRIALSRGLRPD
                     RFLQEIRQYGVTVVSYTWAMLREVIDDPAFSLTGSHPVRLFIGSGMPTGLWKRVCEVF
                     APAHVVEFFATTDGQAVLANVSGAKLGSKGRALPGGGDVELAAYDAEDDLILEDDRGF
                     VRRADTNEVGVLLAHPRGPVDPTASVKRGVFAPADTWVSTEYLFRRDEDGDYWLVDNR
                     GAVIHTERGTVYASPVNDAVGRLGAVDLAVTYGVDANGHQLAVTALALCPGGSIPSAD
                     LNEALADLPVGNPPDIVHVVPEMKLSASYRPLVSPLQAEGIPKPSRNAWYLDPDTNRY
                     KRLTVAVRTEIAGAQQ"
     gene            3532..4077
                     /locus_tag="VFC01_33650"
     CDS             3532..4077
                     /locus_tag="VFC01_33650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019970232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84603.1"
                     /translation="MTLQTSNLRRAVKGVVASGAMAAGLLTGFGAATAFAEPVESCTG
                     EECHKTDPVDPAAQAAAPPVMTADQALAIIAQDYDTGEGGGQLSTLIHDVLKLRALGF
                     KPSNANKEAITKALDYRPNQAPLIEALKETLAYQHRLQAMQQNANNGQQQPGYGFGIG
                     QPAPGVPPQPGQNSGVILGVG"
     gene            4087..4302
                     /locus_tag="VFC01_33655"
     CDS             4087..4302
                     /locus_tag="VFC01_33655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HZN84604.1"
                     /translation="MVDEKLLSILVCPEDRGPLLLVDGEFLYNPRLRRAYRIEDGIPV
                     LLIDEAVAVTDDAEHQRLLDRASGQSG"
     gene            complement(4285..4899)
                     /locus_tag="VFC01_33660"
     CDS             complement(4285..4899)
                     /locus_tag="VFC01_33660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN84605.1"
                     /translation="MTAELERKRPGRPPGTSDTRERILTSARNLFARNGIDKSSIRAI
                     AKDAGVDPALVHHYFGTKTQLFAAAIHIPIDPMQVIGPLREVPVEQLGHTLPSILLAL
                     WDSEMGKGFIAELRSLLAGNDVTLIRSFLQEVITQEVGTRVDNPPGSGPIRIQFVASQ
                     LVGVVMARYILELDPFKSLPVDQIAETIAPNLQRYLTGDLPGLT"
     gene            complement(4901..5719)
                     /locus_tag="VFC01_33665"
     CDS             complement(4901..5719)
                     /locus_tag="VFC01_33665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN84606.1"
                     /translation="MHVTGGSVSVHHPAQHRSRTRITLQGYAATTARILRQLAADHRT
                     IAMMLLVPSLIITLMYFMFQNVPTRPGVPNGFNNACLILLGLFPLFVMFLITSITMQR
                     ERASGTLERILTTPLRRLDLLAAYGTAFGIAAAVQATLTCITAFWVLGFDTAGSPVWV
                     FVIAIVNAVLGVGLGLLCSAFARTEFQAVQFMPLVIAPQLLLAGIIVPRALMPDWLEW
                     ISNVMPASYALEALQQVGAHPELTATAVRDIIVVMGCALVALCLAAATLRRRTP"
     gene            complement(5653..6417)
                     /locus_tag="VFC01_33670"
     CDS             complement(5653..6417)
                     /locus_tag="VFC01_33670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN84607.1"
                     /translation="MMTSSSDELLRRGVEPVIVIDHLRVIRGKRPAVHDLSVQIARGT
                     ITGLLGPSGCGKTTLMRSIVGTQIVTAGSVTVLGHPAGSAPLRRRVGYMPQDPTIYDD
                     LRIVDNVRYFATLYGMDSRAADAAIDAVGLSDHRTALCGNLSGGQRTRASLACALVCQ
                     PDLLVLDEPTVGLDPVLRADLWEQFNTLAKSGITLLVSSHVMDEADRCGDLLLMRDGH
                     LLAHITPDRLREETGCTSLEEAFLSIIRRSTAAAPG"
     gene            6477..7088
                     /locus_tag="VFC01_33675"
     CDS             6477..7088
                     /locus_tag="VFC01_33675"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZN84608.1"
                     /translation="MGADLLAVDPVAAAQRLLGAALTGRGVSAMIVEVEAYGGPLDGP
                     WPDAASHSFRGRGGRNLVMFGPAGRLYTYRSHGIHVCANVVCAFDGVAGAVLLRAAAV
                     EAGLDTAQARRGDMTRPVALARGPGNLCSAVGITMDDNGIDLFDPKSPVRLTLGEDHA
                     GISGPRVGVSKAADRPWRFWLAGRPEVSPYRRSPRAPAPGESD"
     gene            complement(7085..7507)
                     /locus_tag="VFC01_33680"
     CDS             complement(7085..7507)
                     /locus_tag="VFC01_33680"
                     /inference="COORDINATES: protein motif:HMM:NF039954.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5990 family protein"
                     /protein_id="HZN84609.1"
                     /translation="MLLQIRGYHLPGRAWQSHDEHYDNVHVGIQVGKEPRELVRADAE
                     MSSWTIPIDVIMRDGKLDFRGAAVQGRPGDRFIYLTWGDVGEDGTFGMFRRAKLMLAD
                     LEPFAGAEQVIARIGLTDECGGPTCARLKPPALTLSTD"
     gene            7538..8812
                     /gene="tyrS"
                     /locus_tag="VFC01_33685"
     CDS             7538..8812
                     /gene="tyrS"
                     /locus_tag="VFC01_33685"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZN84610.1"
                     /translation="MGTSILDELEWRGLIAQSTDRDALAAELESGPITVYSGFDPTAP
                     SLHAGHLIPLLTLRRFQQAGHRPIVLAGGATGMIGDPRDTGERTLNTADTVADWADRI
                     RGQLERFVDFDDSPTGAIVQNNLSWTSELSAVEFLRDIGKHFSVNVMLDRDTVRRRLE
                     GDGISYTEFSYMLLQANDYVELHKQYGCALQVGGSDQWGNIIAGVRLVRQIAGATVHA
                     LTTPLVTDSEGKKFGKSTGGGSLWLDPEMTSPYAWYQYFINTADADVIRYLRWFTFLS
                     RDELAELEQATAERAHERAGQRRLARELTTLVHGEAATAAVEHASQALFGRGELAQLD
                     EPTLAAALREASVAELKPGGPDTITDLLVASGLSGSKGAARRTIGEGGAYVNNVRIDT
                     EDWVPGPEDFLHGRWLVLRRGKRNIAGVERVG"
BASE COUNT         1536 a         2968 c         3112 g         1570 t
ORIGIN      
        1 cacgtcgtgc ccaccaagca gcacctcggc cagcgccacc cggcgacgct caccacccga
       61 cagcctgctc acctctgcgt cgagctcgac accggacaac agctgcgaaa cgaccgcacg
      121 ggtgtccggc tcggctgccc acacatggtc cggcctgcca ccgacgatga cgtcccggac
      181 agtggcgcca aggagctcgt cgccctgccg caaatacccc acggacaggc cggaggtgtg
      241 cgtgacccgg ccggagtcgg gctgccgggt ctcggtcaac acctgcagca gggtggtctt
      301 gccgtcgccg ttgcgcccga cgacgccgat cgcgtcaccg tcctcgacac ccaggctgac
      361 ctcgtcgagc agggtgcggg tgccgtagcc gacggtcgcg cgctcgacgt tgatcagatt
      421 cgccatcagg cgccgatgat atgtgggcgg cgtgccatga tgaccacggc agtcggggga
      481 ctgacgtggc aataaggggc gtgggcgtgg tggacctgtc ggctataacc aggccagtgg
      541 ggcggctggt ggctaccgcg cagaacgggc tggaagttct ccggtacggc ggcctggaaa
      601 ccggggccgt gccgtcgccg ttccagatga tccagagtgt cgcgatgtac cggctgcggc
      661 gctacttccc accggacgcc cggcccgggg ccaagcggcc agggccgccg gttctgatgg
      721 tgcacccgat gatgatgtcg gccgacatgt gggacgtcac ccgcgacgat ggtgcggtcg
      781 gcatcctgca caaggccggc atcgacccgt gggtcatcga cttcggctca ccggacaagg
      841 tcgagggcgg catggaccgg aacctcgccg accacgtcgt cgcgctgagc gaggccatcg
      901 acaccgtcaa agaggtcacc ggccgcgacg tccatctggc cggctactcc cagggcggga
      961 tgttcgcgta ccagaccgcg gcctaccgcc ggtcgaagga cctcgccagc atcgtcgcgt
     1021 tcgggtcccc ggtcgacacc ctcgccgcat tgccgatgaa catgcccgcc agtttcgcgc
     1081 cggccgccgc gaatttcatg gccgaccatg tgttctcgcg catcgacatc cctgggtggc
     1141 tggcgcgcac cggcttccag atgctcgacc ccatcaagac cgcgcagtca cggcttgact
     1201 tcttgcgcca gctgcatgat cgcgaggcgc tgctgccgcg tgaacagcag cgccgattcc
     1261 tcgcttccga gggttggatc gcgtggtcag gacccgcgat cgccgagctg ctcaagcagt
     1321 tcatcgcgca taaccggatg atgagcggcg gcttctcgat tcacggcgac ctagtgacac
     1381 tgtccgacat cgactgcccg gtgctggcgg tcgtcggcga ggtcgacgac atcgggcaac
     1441 ccgcctcggt ccgcggcatc aaacgggccg cgccacaggc cgatgtgtac gaattcctca
     1501 ttcgtgcagg acatttcggc ctcgtagtgg ggtcgaaggc ttcgacgcag acctggccgg
     1561 ccgtcgcgca gtgggtcaag tggatcgacg gcaccggcga tatgccccac ggggtttcgc
     1621 cgatggcgct gcagcccgcc gaacacgccg aaagcggcgt ctcgctctcg tcccgggtgg
     1681 cacacggcac cgcggccgcc accgagatgg ccttcagcct ggcgcgaacc gccgccgacg
     1741 ccatcgtggc ggcgaacaag tcagcgcgaa cgcttgccat cgagacgtat cgcacgctgc
     1801 cccggctcgc gcgcctcggc cagatcaacg accacacccg aatctcgctt ggccgcatca
     1861 tgtccgagca ggcccgcgac gtacccgaag gggagtgcct gctgttcgac ggccgcgtcc
     1921 acacctttga agcggtggac cgccgaatca acaacgtcgt ccgcggcctg atcgaggtcg
     1981 gtgtgcgcca gggtgcgcac gtcggcgtgt tgatggagac ccgacccagc gcgctcgtcg
     2041 cgatcgcggc gctgtcgcgg cttggcgcgg ttgcggtctt gatgccgccc gacgtcgacc
     2101 tggccgccgt cgcgcggttg ggaggggtgt ccgagatcat cgccgacccc agcaatctcg
     2161 aggcggcccg ccgactggac atgcgggtgc tggtgctcgg cggcggcgaa tcgcgcgacc
     2221 tgcacctgcc tgaacacgtc gacgtcgtcg acatggaaaa gatcgacccc gacgtcgtcg
     2281 acctgcccgg ctggtaccgg ccgaatccgg ggctcgcgcg tgacctcgcg ttcatcgggt
     2341 tcagcgccat cggcggcgaa ctcgtcgccc gccagatcac caactaccga tgggcgctgt
     2401 ccgcgttcgg cacggcgtcg gcagccaacc tcggccgcgg cgacaccgtc tactgcctca
     2461 ccccgctgca tcaccagtcc gggttgctgg tgagcctcgg cggtgcagtg gtcggcggct
     2521 cccgcatcgc gctgtcgcgg gggttgcgcc cggaccggtt cttgcaagag atccgtcagt
     2581 acggcgtgac ggtggtgtcg tacacctggg cgatgctgcg tgaggtcatc gacgatcccg
     2641 cgttctcgct gaccggcagc cacccggtgc ggctgttcat cggttccggc atgccgacgg
     2701 gtctgtggaa gcgtgtgtgc gaggtgttcg cgcccgcgca tgtggtggag ttcttcgcca
     2761 ccaccgacgg gcaggcggtg ctggccaacg tgtcgggcgc caagctgggt agcaagggca
     2821 gggccctgcc aggcggcggc gacgtcgaac tggccgccta cgacgccgag gacgacctga
     2881 tcctcgagga cgatcgcgga ttcgtccgca gagccgacac gaacgaggtc ggcgtgctgc
     2941 tggcccatcc gcgcggtccc gtggatccga cggcatcggt caaacgcggc gtgttcgcgc
     3001 ccgccgacac ctgggtgtcc accgaatacc tcttccgccg cgacgaggac ggcgattact
     3061 ggctcgtcga caaccgtggc gcggtcatcc acacggagcg cggcacggtc tatgcctcac
     3121 ccgtcaacga tgctgtcggc cggctcggcg cggtggatct ggccgtgacg tacggggtgg
     3181 acgcgaatgg ccaccagctc gccgtcaccg cgttggcgtt gtgtcctggt ggcagcattc
     3241 cgtcggccga tctgaacgag gccctggccg atctgccggt cggcaacccg cccgacatcg
     3301 tgcatgtggt gccggagatg aagctcagcg cgtcctatcg acctctggtc agcccgctgc
     3361 aggccgaggg gatccccaag ccgtcgcgta acgcctggta tttggatccc gatacgaatc
     3421 ggtacaagcg cttgaccgtc gcggtacgga ccgagatcgc cggcgcccaa caatgagccg
     3481 tgccttgcgg ccgggtcgtt gttaggctcc cgctggcggc aggaggagta aatgacattg
     3541 cagaccagta atctgcggcg tgcagtcaag ggtgtcgtgg ccagcggcgc tatggccgct
     3601 ggtctcctca ctggtttcgg tgcggcgacg gcgttcgccg agcccgttga gagctgcaca
     3661 ggggaggaat gccacaagac cgacccagtg gacccggctg cgcaggctgc cgccccaccg
     3721 gtcatgacgg ccgaccaggc gcttgccatc atcgcccagg attacgacac gggcgagggc
     3781 ggcggtcagc tctcgacgct gatccacgac gtgctaaaac ttcgcgccct gggcttcaag
     3841 ccctcgaatg cgaacaagga agcgatcacg aaggcgttgg attaccgacc gaaccaggct
     3901 ccgctcatcg aggctttgaa ggagacgctg gcctaccagc acagactgca ggcgatgcag
     3961 cagaacgcca acaacggcca gcagcagccg gggtacggct tcggcatcgg ccagccagcg
     4021 ccgggcgtgc caccccagcc tggccagaac tcgggcgtaa tcctcggagt cggctagcct
     4081 acggccgtgg tcgacgaaaa actgctgagc atcctcgtct gtccggaaga tcgtgggccg
     4141 ctgttgctgg tcgacggcga gtttctgtac aacccgcggc ttcgccgcgc ataccgcatc
     4201 gaggacggca tcccggtact gctcatcgac gaggcggtcg cggtgaccga tgacgccgag
     4261 caccagcggc tgctggaccg agcctcaggt cagtccgggt agatcgccgg tcagatagcg
     4321 ctgcaggttg ggcgcgatgg tttcggcgat ctggtcgacc ggcagcgatt tgaacgggtc
     4381 caattccagg atgtaacggg ccattacgac gcccactaac tgcgacgcga cgaactggat
     4441 gcggatgggc ccgctgcccg gcggattgtc gacgcgggtt ccgacctcct gagtaatcac
     4501 ttcttgcaga aacgaacgaa tcagcgtgac gtcgttgccg gccagcaggg atcgcagttc
     4561 tgcgatgaag cccttgccca tttcggaatc ccacaacgcc aacaggatcg acggcagcgt
     4621 gtggccgagt tgctcgaccg ggacttcacg cagcggtccg atcacctgca tcgggtcgat
     4681 cggaatgtgg atcgccgcgg cgaacagttg ggtcttggtg ccaaagtagt ggtgcaccaa
     4741 cgcggggtcg acacccgcgt ccttcgcgat ggcacggatc gacgacttgt caatgccgtt
     4801 gcgggcaaac aggttacggg cgctggtgag gatgcgctcg cgggtgtcgg acgttccggg
     4861 cgggcgcccg ggacgcttgc gttcgagttc ggcagtcacc ctatggcgtc cgtctccgca
     4921 acgtcgctgc cgccaggcac agggcgacga gggcacaccc catcaccacg atgatgtcgc
     4981 gcaccgccgt tgcggtgagc tccggatgcg caccgacctg ctgcagcgcc tccaacgcgt
     5041 agctggcggg catcacattg ctgatccatt ccaaccagtc tggcatcagc gcacggggaa
     5101 cgatgatccc ggccaacagc agttgcggcg cgatcaccag cggcatgaac tgcacggcct
     5161 gaaactcggt gcgcgcgaac gcactacaca acaggccgag gcccacacct agcaccgcgt
     5221 tcacgatcgc gatcacgaac acccacaccg gactgcccgc ggtgtcaaag ccgagcaccc
     5281 agaacgccgt gatgcacgtc agggtcgcct ggaccgccgc ggcgatcccg aacgcggtcc
     5341 cgtacgcggc gagcaggtcc agccggcgaa gcggtgtggt caggatccgt tccagcgtcc
     5401 ccgacgcccg ttcccgctgc attgtgatcg acgtgatgag gaacatcacg aacagtggga
     5461 acaggcccaa caggatcagg cacgcattgt tgaatccatt cggcaccccc ggccgagtgg
     5521 gcacattctg gaacatgaag tacatcagcg tgatgatcag gctcggcaca agcagcatca
     5581 tcgcgatcgt ccggtggtcg gcggcgagct gacgcaggat tcgcgcggtg gtggcggcgt
     5641 agccctgaag cgttatccgg gtgcggctgc ggtgctgcgc cggatgatgg acagaaacgc
     5701 ttcctccagt gacgtgcatc ctgtctcctc tcgtagtcgg tccggggtga tgtgagcgag
     5761 cagatgcccg tcgcgcatca gcaacaggtc gccgcagcgg tccgcctcgt ccatgacgtg
     5821 gctggatacc agcagcgtta ttccgctctt cgccagggtg ttgaactgct cccacaggtc
     5881 ggcgcgcagt accgggtcga gcccgacggt gggttcgtcg agcacgagca gatcgggttg
     5941 gcagaccagc gcgcaggcca acgaggctcg cgtccgctgc ccgccagaaa gattgccgca
     6001 cagcgccgtt cgatgatcgc tcagcccaac agcatcgatg gcagcgtcgg cagcgcgtga
     6061 atccatgccg tacaacgtgg cgaagtagcg gacgttgtcg acgattcgaa gatcgtcgta
     6121 gattgtgggg tcctggggca tgtatccgac ccgtcggcgc aggggagccg atccggcggg
     6181 atggcccagc accgtgacgg atccggcagt gacgatctgc gtgccgacga tggatcgcat
     6241 cagggtggtc ttgccgcacc ccgacgggcc gagaagcccg gtgatcgtgc cgcgcgcgat
     6301 ctgcaccgac agatcgtgta cggccggccg tttgccgcgg atgacacgga gatggtcgat
     6361 gacgatgacc ggttcgacac cgcgtcgcag taattcatca ctcgatgaag tcatcatacg
     6421 atgaataatc ctccccgatt cccggtgtgt caacggtcgc aggcagaatc tctccggtgg
     6481 gtgcagatct gttggccgtc gatcccgttg ctgcagctca gcggctattg ggagcggcgc
     6541 tcaccgggcg cggcgtcagc gccatgatcg tcgaggtgga ggcctacggc ggcccgctcg
     6601 acgggccctg gcccgatgcc gcgtcgcact cgtttcgcgg ccgcggtggt cgaaacctgg
     6661 tgatgttcgg ccccgccggg cggctgtaca cgtaccgcag tcacggcatt cacgtctgcg
     6721 ccaacgtggt gtgcgctttc gatggcgtcg cgggcgcggt cctgctgcgg gcggcggcgg
     6781 tcgaggccgg cctcgatacg gcgcaggccc ggcgcggtga tatgacgcgg ccggtcgcgt
     6841 tggcgcgtgg acccggcaat ctgtgctcgg ctgtcggaat caccatggac gacaatggaa
     6901 ttgacctatt cgatccgaag agcccggttc gtttgaccct tggcgaggat cacgcgggca
     6961 tcagtggccc gcgcgtcgga gtcagcaagg ccgccgaccg gccgtggcgt ttttggttgg
     7021 ccggcaggcc ggaggtgtcg ccgtatcggc ggagcccgcg cgcaccggct cccggcgaga
     7081 gtgactaatc cgtcgacagc gtcagggcgg gcggtttgag ccgggcgcac gtcgggccgc
     7141 cgcattcgtc ggtgaggccg atcctcgcga tcacctgctc agcgccggcg aacggctcca
     7201 ggtcggcgag catcagtttg gcccgtcgga acatgccgaa ggtcccgtcc tcaccgacgt
     7261 cgccccaggt gagatagatg aaccggtcgc caggcctgcc ctgcaccgcc gcaccgcgaa
     7321 aatcgagttt gccgtcgcgc atgatgacgt cgattgggat cgtccacgac gacatctcgg
     7381 cgtcggcgcg cactagctcg cgaggctcct tgccgacttg aatgccgacg tggacgttgt
     7441 cgtaatgctc gtcgtgggac tgccaggcgc ggcccggcag gtgataaccc cggatctgca
     7501 acaacatcgg catcgaccgt aagggaagat cgatcgcatg ggtacgtcga ttctcgacga
     7561 gttggaatgg cgtggactga tcgctcagtc caccgaccgt gatgcactgg cggccgaatt
     7621 ggaatccgga cccatcactg tctattcggg cttcgacccg accgctccga gcctgcatgc
     7681 cggacacctc atcccgctgc tgacgctgcg gcgctttcag caggccgggc accggccgat
     7741 cgtgttggcc ggcggtgcca ccggcatgat cggcgatccg cgcgacaccg gcgagcggac
     7801 cctaaatacc gccgacaccg tcgcggactg ggctgatcgc attcgcgggc agctggagcg
     7861 tttcgtcgat ttcgacgact cgccgacggg cgccatcgtg cagaacaacc tgtcgtggac
     7921 gagcgagctg tccgcggtcg agttcctgcg cgacatcggt aagcacttct cggtcaacgt
     7981 catgctcgat cgcgacacgg tccggcgccg cctggagggc gacggcatct cctataccga
     8041 gttcagctac atgctgttgc aggccaatga ctacgtggag ctgcacaagc agtacggatg
     8101 tgcactgcag gtcgggggct cggatcagtg gggcaacatc atcgccggag tgcggctggt
     8161 ccggcagatt gcgggtgcga cggtgcacgc gttgacgacg ccgctggtga cggattcgga
     8221 aggcaagaag ttcggcaagt cgaccggcgg cggcagcctc tggctcgatc cggagatgac
     8281 gagcccgtac gcctggtacc agtacttcat caacaccgcg gacgccgacg tcatcaggta
     8341 tctgcggtgg ttcacgttct tgtcgcgcga cgagctggcc gagctggagc aggcgaccgc
     8401 cgaacgggcg cacgagcgag cgggtcaacg tcggttggcg cgcgagctga ccacgctggt
     8461 gcacggtgag gctgcgacgg ccgcggttga acatgccagc caggcgctgt tcgggcgggg
     8521 ggagttggcg cagctcgatg agccgacgct cgcggcggcg ctgcgggagg catcggtcgc
     8581 ggagctcaag ccgggcggac cagacaccat cactgacctg ctggtggcca gcggattgtc
     8641 ggggagcaag ggcgcagcgc gtcggacgat tggggagggc ggcgcctacg tcaacaacgt
     8701 gcggatcgac accgaggact gggtcccggg gccggaggac ttcctgcacg gccgctggtt
     8761 ggtgctgcgg cgcggcaagc ggaatattgc cggcgtcgag cgcgttggat gatttgtcgg
     8821 agtcgacgca cgctagtgtg cgtacgatgg cggcgaagcc gaaagacacg ccccatcagc
     8881 ccatttgact cccagttatc cctcacgtaa cttatcggag tcgctgcgac acgggcctgg
     8941 cccgaagagc gcggcggctc ccagagggaa gccccacttc ggtgggttcc tgatcgtccg
     9001 cactacgagt acgcggcttt tgccgcgggc tagtcgcgcg gccgagcggg tgtgttgttt
     9061 gagaactcaa tagtgtgttt ggtggttttt gttttgttgt tgttttttgc cgcattctga
     9121 ctccccgtgt tggggtgcgg tgttttttga tgccagtttt tggtgtcttg ctttgatcag
     9181 tttctc
//
LOCUS       DASRJA010000746        12367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1236532,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000746 DASRJA010000000
VERSION     DASRJA010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12367
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236532"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..279
                     /locus_tag="VFC01_33690"
     CDS             85..279
                     /locus_tag="VFC01_33690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84611.1"
                     /translation="MGSFDVLLNDERTQLEAFVEEYRSAIENTLDGLTDEEARHRLVP
                     TGRPARRPGRPWPTSRCTPS"
     gene            249..395
                     /locus_tag="VFC01_33695"
     CDS             249..395
                     /locus_tag="VFC01_33695"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HZN84612.1"
                     /translation="MADLSLYAVVTGHRAGPRTLHWVYLQVLRELAHHCGHADILREQ
                     ILAG"
     gene            complement(411..1652)
                     /locus_tag="VFC01_33700"
     CDS             complement(411..1652)
                     /locus_tag="VFC01_33700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN84613.1"
                     /translation="MPTAVPVDLSDTALWQNGFPDDLFVELRRERPIFHHELTAGVAK
                     TVNRDFWMTTKHRHAQRIHRDTDSFTAADGPLIQPIGVMTDFPTIIHMDPPELTKRRR
                     VMSHAFTPKAIGKLEEGIRTRAASLIDRLLAAGGGDWIEDVADVLPMTVIGDIIGIPD
                     DDRPEIFDTLDRILKAANSPDARATEQEQLELFGKVFTYALELTAEKRRNPADDIWST
                     LATAVVTDENGEQLSIPANELEIFFYVLTFAGSDTTKNALASGLQAFVANPAEIARYR
                     ADEAIRPSAVEEVLRWATPVAFWTRSTKVDVEIDGVTIPKGDRVVSMLRSANRDEEVF
                     GDPFTFDIGRVENQHVTFGGGGPHHCLGAMLARAEIRAVLDELLCRADDIMVGEPTVS
                     YPNLTNNMSIFDAMPISVRSH"
     gene            1758..2870
                     /locus_tag="VFC01_33705"
     CDS             1758..2870
                     /locus_tag="VFC01_33705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZN84614.1"
                     /translation="MADTVADTTDPSEREFGPSGISLSTYRFPTGWFIVGFASDLAAG
                     QVKRAHYFGEELVIFRTESGQINVLDAYCQHLGANMGVGGTVEGERIVCPWHGWRWNG
                     DGTNALIPYSKIGCKQNVRIKTYPVTEWYGFILVWHERHGRAPYWQPPVLPELETNEY
                     YPLHPHTRMVNRVKVHAQMIIENAADPYHVQYVHKAANPANTASFEVSGYHLHATVNA
                     DFGGGRAKTWLTPNGPVEAKIIYDNYSLGLGVVRFPSDLVATVQVTGQTPVDEDYTDY
                     FYTQASIREPGDTGDLPTGRAAKFLQLQQEVIKQDFFTWENMKYLEKPNLAPEEARDY
                     AALRRWAHRFYPGTKPAPDDFGYTSGGEPDPAAARA"
     gene            2870..4441
                     /locus_tag="VFC01_33710"
     CDS             2870..4441
                     /locus_tag="VFC01_33710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN84615.1"
                     /translation="MPSAFDVERFTVPAVLDRRAEQFGDRVMMSIAGTPITFTQMRDR
                     SCAAANVLAGLGVSRGQTVALFTGTCPDWVYFWLGAARIGAVPAAVNAASKGDFLVHT
                     LRLSQAAVAVTDADRHARLTEVAGDVDTLRSTLVQGDSLTDMLNAASIEPPSGAAAEP
                     DDIALFFTSGTTGPSKAVATTWHYLFTVAATAASSWELAQGEVLWTAMPLFHLSAAPT
                     VLAPMLVGGTSVVATTFHPSEVWDEIRSCGAAGFAGAGAMVSMLWNLPPDPRDAEIGL
                     RFISAAPIAADMYRAIEERYRCRIVTMYGMTEAFPIAYKAVSDEGVPGTSGRVNPAFD
                     VRIVDLLGQPVPQSMVGEIACRARKPHAMSEGYVASASGGPPLQIDPHPEWFRTGDLG
                     VLDSDNNLTYVDRAKDSLRRRGENISSVEVEQTVMRHPAVWEAAAVGVASDLGEDDIL
                     VVVTVRPDAVVEYAELLDFCAARMPYFCVPRYLEVLDEIPKNVIGRVRKDVLRNRGLG
                     AGAWDREAHGYVLSR"
     gene            4458..4871
                     /locus_tag="VFC01_33715"
     CDS             4458..4871
                     /locus_tag="VFC01_33715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN84616.1"
                     /translation="MTLTYQQQFVLDGLAGRAAIINLDARHNRLYSAGDVAGWIATFR
                     HAGATYTRAGESFTDLHEAFDGGNGVRLVTVDHEITVDGVDATQQSVALLFRDNELQA
                     SGTYTDRLIYERGGWYFTSRVLEWDLVPRDSALPV"
     gene            4925..5398
                     /locus_tag="VFC01_33720"
     CDS             4925..5398
                     /locus_tag="VFC01_33720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HZN84617.1"
                     /translation="MVGARTEPRFAGTAVSGARIQHFASMVRDPNPGYWDAEFAKSVW
                     GGLVAPPALLTGLLMPPPWVPNGQPPTASISVRVPLPGTAIINASNDAEYLTPVLEGD
                     ILSVVEEVVAVSPEKKTRLGSGHFIETLETYYRDDGAIVATCTNTLFRFTPAGAR"
     gene            5395..5826
                     /locus_tag="VFC01_33725"
     CDS             5395..5826
                     /locus_tag="VFC01_33725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020786650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84618.1"
                     /translation="MTWDDVSVPAQLPEVVDQISYERVVMNAATTWDYVPWHYNPQYA
                     KDHGHPTIFVNTMHLAGFIDRIATDFGGPYSRVVRRKVSLLGSIYAGDSMVARGRVIG
                     KRIDTAGGARRYLVDLEVSIFNQHDELCCPAEVAVELTSGP"
     gene            complement(5813..6646)
                     /locus_tag="VFC01_33730"
     CDS             complement(5813..6646)
                     /locus_tag="VFC01_33730"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN84619.1"
                     /translation="MTEVVVERHGGVVVCRLNRPQQRNGLTGTLLAEYLAALGEASRD
                     DTVRAVVTTGEGDAFSAGADMSDLDSDAARNGLNALMHDKLGLTDTLSTADRAFDRLG
                     IGRETLAIKNFDKPLIAAVNGAAAGGGLALAMLHDIRIASERATFTSAFVRIGLVAEM
                     GLSYTLPRAIGLEAAMDMMYSGRLVGADEALEMGLIRRVVSHDRLMAETMAYAEQIAA
                     QAPIAVQFTKRTLSRSLDNSLLAQLELEWPYQVAAFDTDDAREGIAAFRERRPPRFTG
                     R"
     gene            complement(6650..8107)
                     /locus_tag="VFC01_33735"
     CDS             complement(6650..8107)
                     /locus_tag="VFC01_33735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZN84620.1"
                     /translation="MRSLVYDKLYIGGQWQSPATAQRLSVISPHTEEPIGETPEASPE
                     DVDKAVAAARTAFDDGPWPRLTPAERMEKIEKLAALYSGQTDEMADLITAEMGSPRSF
                     SRLGQATGAVAQMHLNLATAREFPWAERRQGLFGEVHVRRAPVGVVGAIVPWNVPQFL
                     IMPKLIPALIAGCTVVVKPAPETPLDAMWLAEMLDEIDLPEGVVSIIPGGRETGETLV
                     RHPGVDKISFTGSSSTGRRIAALCGEQLKRVSLELGGKSAAIILDDADIDRTVNHLKM
                     ASLMNNGQACVAQTRILVSDRRHDEVVDALAEMMSSLQVGDPADEATDIGPLVAERQQ
                     DRVQGYIETGVDEGARIVLGGTDKPRERGWYVQPTLFADANNEMRIAREEIFGPVLTV
                     LKYSDERDAIRIANDSDYGLAGSVWTADIAHGLEIAAEIRAGTYGINMYTLDTSCPFG
                     GFKQSGIGREFGSEGLSEYVELQSTVSAGKLPDLS"
     gene            complement(8104..9066)
                     /locus_tag="VFC01_33740"
     CDS             complement(8104..9066)
                     /locus_tag="VFC01_33740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN84621.1"
                     /translation="MRDIGKRFPFDAYPKGWFQVAYSRDVDAGQVVGLHYFGRRLICY
                     RGASRTPYVLDAYCPHLGADIAVGGTVADDCIVCPFHGWRFDGAGSNVEIPYAKTVNR
                     TARLRAWPTVERAGAIFVWHSQAATEPEWELPKIPESEDAAFTFHAPDSARWRFRSHP
                     QEVFENTVDIAHFATVHGVSGFGDLDLETDGHRLRAIAEVTFQTPRGPVSGAVDSELF
                     GMGLDVVRHRGLGRSCTLLTVTPIDGEFVDARYTFFVQTDPETGEMTRMGLGFARDFC
                     KQIEQDIPIWENKIYRDRPQLARGEGAITEFRAWARQSYEEAIA"
     gene            9137..10300
                     /locus_tag="VFC01_33745"
     CDS             9137..10300
                     /locus_tag="VFC01_33745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HZN84622.1"
                     /translation="MDDRDWRNYPFDLVPGDAALSFPEAEGNHPDCESDTWFIAGELS
                     ADSGRRFAFLTIFNKNRPGGSIVADFYTFALFDLDNGGYGTYTDYDMPPANMAPGARP
                     KLSVATGHLDMTYDSGAGQAVWRACRDEQHLLVPYTYDVSLVGTDQFGHAMRLDLHVT
                     PSRAPVPLGGSAHNGKITCFGQEDTYSYFQTRMVMTGTVRWGAAEESVTGETGHVDRQ
                     WFPLVANSGGATGDIRSRAHEWRTINLDNGVDLSIWRQFDRTDNNALQPFSGATTSSP
                     DADPEYADDIEVVVDSYVRWPESIRTLVPPPAALRYMPDRHRLTSRKLDLDLTGEPLV
                     AAPAHALPLEYMEGPYRYRGTMRGKPVSGFAFYERSLALYRDWELAEIADAHV"
     gene            complement(10234..11406)
                     /locus_tag="VFC01_33750"
     CDS             complement(10234..11406)
                     /locus_tag="VFC01_33750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014711120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN84623.1"
                     /translation="MHRDQLIDLTRRAVKLARDKTTDLTLAEHTVAADTYTSNDRHAL
                     DTAMLMASPQLVGYSSELPQPGTYCTKTVMGRSILLTRSADNAVRAFDNICLHRQSRI
                     ADGCGAARRLACPYHSWTYDLDGNLVGVPGKEGFPETRSGPARLTELPAAECAGFLWI
                     SLDRDATLDIPAFLGPLAGELESWGIGQWSPMGEKVLDCPINWKLAIDTFAENYHFAT
                     VHKATFATIARSNCTVFDSFGVHHRLVFPLNGILDLEDVPEEQWDPLQNMVVIYALFP
                     NIVLSCTIANGELFRVYPADVPGRSITVHQNSTPLDLSDESVAAGAAAVFDYAHATVR
                     DEDYALVAGLQANLESGARERLVFGRNEPGLQHRHQTWASAISANSQSRYSASERS"
     gene            11528..>12367
                     /locus_tag="VFC01_33755"
     CDS             11528..>12367
                     /locus_tag="VFC01_33755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HZN84624.1"
                     /translation="MTFRGGMSSGWVKRLAIVAFATAVVPGLVSLAGGTVTAGAFSSP
                     GLPIEYLDVPSQGMGRNVKVEFMSGGPTAPALYLLDSMEAGDDFNGWDINTQAFDWYN
                     GSGLSVVMPVGGKSSFYSDWYGPAVGNAGTYTYKWETFLTQELPAWLAANKGTPQTGN
                     AIVGFSMGGSSALVLAAYHPQQFIYAGSISGFLNLSADPGQVGIAMMWNGGFSREAMW
                     GPPGDPAWARNDPTLQAGRLAANGTRLWVYCGSGTPTDPALASPEAPIGGLGFLEGMA
                     IDSN"
BASE COUNT         2126 a         3986 c         4056 g         2199 t
ORIGIN      
        1 ggtcggcacc cgaccgaagg gcctgtgagt aagacctggt ctcagaagta tggtgtgtcc
       61 gcggcggcat gacgggaggt tctgatgggg tcgttcgacg tgcttctgaa cgacgagcgc
      121 acccaactcg aagcgtttgt cgaggagtac cgcagcgcca tcgagaacac acttgatggc
      181 ctcaccgacg aggaagcccg tcaccggctc gtgcccacag gcaggcctgc gcgacggccc
      241 gggcggccgt ggccgacctc tcgctgtacg ccgtcgtgac gggccatcgc gccgggcccc
      301 gcacattgca ctgggtttac ctgcaggtac tgcgcgaact ggcgcaccac tgcggtcacg
      361 ccgacatcct gcgcgaacag atactcgctg gctgagttct gagatcaggt ctaatgcgac
      421 cgcactgaaa tcggcattgc gtcgaatatc gacatgttgt tggtcaggtt ggggtaactg
      481 accgtcggct cgccgaccat gatgtcgtct gcgcggcaaa gcaattcgtc gagcaccgca
      541 cggatctccg cgcgtgccag catcgcgccg aggcaatggt gcggcccgcc gccgccgaac
      601 gtcacgtgct ggttctccac ccggccgatg tcgaaggtga acggatcgcc gaacacctct
      661 tcgtctcgat tggctgagcg cagcatcgac accaccctgt cgcccttcgg gatggtgaca
      721 ccgtcgatct ccacgtcgac tttggtgctg cgggtccaga acgccactgg tgtcgcccaa
      781 cgcaggactt cctcgaccgc actcggccgg atcgcctcgt cggcgcgata ccgcgcgatc
      841 tcggccgggt tcgcgacgaa cgcctgcagt ccggacgcca acgcgttctt cgtggtgtcg
      901 ctgcccgcga acgtcagcac gtagaagaag atctcgagct cgttggccgg gatcgacagc
      961 tgctcgccgt tctcgtcggt gacgaccgcg gtggccagcg tgctccagat atcgtcggct
     1021 ggattgcgcc gcttctccgc ggtgagttcg agcgcgtatg tgaagacctt gccgaacagt
     1081 tcgagctgct cctgttcggt cgcgcgcgcg tccggtgaat tggccgcctt caggatccgg
     1141 tccagcgtgt cgaagatctc cggccggtca tcgtcgggga tcccgatgat gtcgccgatc
     1201 accgtcatcg gcagcacgtc ggcgacgtcc tcgatccagt ccccaccacc ggccgcgagc
     1261 agccgatcga tcaatgatgc cgcgcgcgtg cgaatgccct cctcgagctt gccgatggcc
     1321 ttcggcgtga acgcatgcga catcaccctg cgccgcttgg tcagctcggg cgggtccatg
     1381 tggatgatcg tcggaaagtc cgtcatcacg ccgatgggtt ggatgagtgg gccgtctgcg
     1441 gcagtgaacg aatccgtatc gcgatgaatc cgttgcgcgt gccggtgctt ggtcgtcatc
     1501 cagaaatcgc ggttgaccgt cttggcgacg ccggcggtca gctcgtgatg gaagatcgga
     1561 cgttcgcggc gcagctcgac gaacaggtcg tcgggaaaac cgttctgcca caacgcagta
     1621 tccgataggt cgacgggaac agccgttggc atgcaagctc ctcgctacgg tggccttgac
     1681 ctagaatgcc taaaatagta aaaataggca atggacaggt tctgacctgg cccgatggcg
     1741 tgcggacgga gagagcagtg gcggatacgg ttgcggacac cacggacccg tcggagcgcg
     1801 aattcggtcc cagcggcatc agcctgtcga cataccgctt tccgaccgga tggttcatcg
     1861 tcggatttgc ctccgacctg gctgctggcc aggtcaagcg cgcccactac ttcggcgaag
     1921 agctggtgat cttccgcacc gagtcgggcc agatcaacgt gctcgacgcg tattgccaac
     1981 acctcggcgc caacatggga gtcggcggca ctgttgaggg tgagcggatc gtctgcccgt
     2041 ggcacggctg gcggtggaac ggcgacggca ccaacgcact gatcccgtac agcaagatcg
     2101 gctgcaagca gaacgtccgg atcaagacct acccggtcac cgagtggtac ggcttcatcc
     2161 tcgtctggca cgagcggcat ggccgcgcgc cctactggca gccgccggtg ctgcccgagt
     2221 tggagaccaa cgagtactac ccgctgcacc cgcacacccg gatggtcaac cgggtcaagg
     2281 tgcacgcaca gatgatcatc gagaacgccg ccgacccgta ccacgtgcaa tatgtgcaca
     2341 aggcggccaa tcccgcgaac accgcctcgt tcgaagtcag cggttaccac ctgcatgcga
     2401 ccgtcaacgc cgatttcggt gggggacggg cgaagacgtg gctgacgccg aacggccccg
     2461 tcgaggcaaa gatcatctac gacaactact cattggggct gggtgtcgtg cgcttccctt
     2521 cggacttggt ggcgaccgtc caagtgaccg gtcagacccc ggtggacgag gactacaccg
     2581 actacttcta cacgcaggcc tcgatccgcg aacccggcga caccggcgac ttgccgactg
     2641 gccgggccgc gaaattcctg caactgcagc aggaagtcat caaacaggac ttcttcacct
     2701 gggagaacat gaagtatctg gagaagccca acctcgctcc cgaggaggcg cgggattacg
     2761 ccgcgttgcg gcggtgggcg caccggttct atccgggcac caagccggcg ccagacgact
     2821 tcgggtacac ctccggcggc gaacccgacc cggcggcagc acgggcgtga tgccctccgc
     2881 attcgatgtc gaacgcttca ccgtcccggc ggtgctcgac cgacgggcgg agcagtttgg
     2941 cgatcgcgtg atgatgtcga tcgcgggcac ccccatcacc ttcacgcaga tgcgtgaccg
     3001 ctcgtgcgcg gcggccaatg tgcttgcggg tcttggggtc agccgggggc agaccgtcgc
     3061 gctgttcacc ggcacctgcc cggattgggt gtacttctgg ctcggcgccg cccgcatcgg
     3121 cgcggtgccc gcagcggtca acgcagcgag caagggcgac ttcctggtcc atacgttgcg
     3181 gctgtcccag gccgcggtgg ccgtgaccga tgctgatcgg catgcccgcc tcaccgaggt
     3241 ggccggcgac gtcgacactc tgagatcaac actggttcaa ggtgattcgc tcacagatat
     3301 gctgaacgcc gcgtcgatcg agccgccctc tggggcagct gcggaaccag acgacatcgc
     3361 cctgttcttc acgtccggca cgacgggacc gtcgaaggcc gttgccacca cgtggcacta
     3421 cctattcacc gtggccgcca ccgccgcctc gtcgtgggaa ttggcccagg gtgaagtgct
     3481 gtggaccgcc atgccgctgt tccacctcag cgcggcaccc acggttctgg cgcccatgct
     3541 ggtcggcggg accagcgtgg tggccacgac attccaccca agcgaggtat gggacgagat
     3601 acggtcgtgc ggtgcagcgg gtttcgccgg cgcaggcgcg atggtgtcga tgttgtggaa
     3661 cttgccgccc gatccgcgcg acgccgagat cggcctgcgg ttcatctccg cggcacccat
     3721 cgcggccgac atgtaccgcg ccatcgagga gcgctatcgc tgccgcatcg tgacgatgta
     3781 cggcatgacc gaggcctttc cgatcgccta taaggcggtg tcggacgagg gggtgccggg
     3841 gacatcgggc agggttaatc ccgctttcga tgtgcgtatc gtcgatttgc tcggacagcc
     3901 cgtaccccag agtatggtag gggagatcgc ctgccgggcg cggaaaccac atgcgatgag
     3961 cgaggggtat gtggcgtcgg cgtccggcgg gcctccattg caaatcgacc cgcatccgga
     4021 atggttccgt accggcgacc tcggcgtgct ggacagcgac aacaacctga cctacgtcga
     4081 ccgggccaag gactcgctcc gacggcgggg cgaaaacatc tcctccgttg aagtcgaaca
     4141 gacggtgatg cgccatccgg ccgtgtggga ggcggcggcg gtcggcgttg ccagcgatct
     4201 cggagaagac gacatcctcg tcgtggtcac ggtgcgaccc gacgcggtcg tcgagtacgc
     4261 cgagctgctg gacttctgcg cagcccgcat gccctacttc tgcgtgcctc ggtacctgga
     4321 ggtgctcgac gagatcccga agaacgtgat cgggcgcgtc cgcaaggacg tgctccgcaa
     4381 cagaggtctg ggcgccggcg cctgggaccg cgaagcgcac ggctacgtgc tgagccggta
     4441 ggacgggaga caagtacatg acgttgacgt accaacagca gttcgtgctc gacgggctgg
     4501 cggggcgcgc ggccattatc aacctcgacg cgaggcacaa ccggctgtac tcggcggggg
     4561 acgtcgcggg ctggatcgcg acgttccggc acgcgggcgc gacgtacacg cgcgcgggag
     4621 agtcgttcac ggatctgcac gaggccttcg acgggggcaa cggcgtgcgc cttgtgaccg
     4681 tcgatcacga gatcaccgtg gacggcgtcg acgcgaccca gcagagtgtc gcactgctgt
     4741 tccgcgacaa cgaacttcag gcctccggta cctataccga ccggctcatc tacgagcgcg
     4801 ggggctggta cttcacgtcg cgggtactcg agtgggacct cgtgccgcgg gatagcgcgc
     4861 tgccggtgtg accgcagacc tgtcctacga gttcgcgttc ggcacgtacg aggacgcact
     4921 tcagatggtc ggcgcgcgaa ccgagccgag gttcgcaggc accgcggtca gcggcgcgcg
     4981 catccagcac ttcgcctcga tggtccgcga ccccaacccg gggtattggg acgccgagtt
     5041 cgcgaaatcg gtgtggggtg gcctggtcgc gccgcccgcg ttgttgaccg gtctgcttat
     5101 gccgccgccg tgggtgccga atggtcagcc tcctaccgcc tccatctcgg tgcgggtacc
     5161 tcttcccggc accgcgatca tcaacgcatc caacgacgcc gagtatctga cgccggtgtt
     5221 ggagggcgac attctcagcg tggtggagga agtggtggcg gtgtcgccgg agaagaaaac
     5281 ccggttgggc agcgggcatt tcatcgagac cctggagacc tactaccgcg acgacggcgc
     5341 catcgtcgcc acatgcacga acacgttgtt tcgtttcacc ccggcaggcg cccgatgacg
     5401 tgggacgacg tgtcggtgcc cgcgcaactt cccgaggtcg tcgaccagat cagctatgaa
     5461 cgagtggtca tgaacgccgc taccacgtgg gactacgtgc cgtggcacta caacccgcag
     5521 tacgccaaag accatggaca tccaacgatt ttcgtcaaca ccatgcattt ggctggattc
     5581 attgaccgga tcgcaaccga cttcggcggt ccgtacagcc gggtggtcag gcggaaggtg
     5641 tcgctgctcg gctcgatcta tgcgggcgat tcgatggtgg cgcgtggccg cgtgatcggc
     5701 aagcgcatcg acaccgccgg gggtgccagg cgatacctgg tggatctcga ggtctctatc
     5761 ttcaaccagc acgacgaatt gtgctgtccc gcagaggtgg cggtcgaact gactagcggc
     5821 ccgtgaagcg cggcgggcgg cgctcccgaa acgccgcgat gccttccctg gcatcgtcgg
     5881 tatcgaacgc ggcgacctgg tacggccact ccaattccag ctgagcgagc aacgagttgt
     5941 cgagcgaacg gctcaaggtt cgcttcgtga actggaccgc gatcggggcc tgcgccgcga
     6001 tctgctcggc gtaggccatc gtctcggcca tcaggcgatc gtgggagacc acgcgtcgaa
     6061 tcaggcccat ctccaacgct tcgtcggcac cgaccagtcg accgctgtac atcatgtcca
     6121 tcgcggcctc gagcccgatc gcgcgcggca gtgtatagct caggcccatt tcggcgacaa
     6181 gcccgatgcg gacgaacgca ctggtgaagg tggcgcgttc ggatgcgatc cggatgtcgt
     6241 gcagcatggc cagtgccaag ccgccgcctg ccgccgcacc atttaccgcg gcgatcagcg
     6301 gcttgtcgaa gttcttgatt gccagtgtct cccggccgat tccgagtcga tcgaacgcgc
     6361 ggtcggccgt gctgagcgtg tcggtcaggc cgagcttgtc gtgcatgagc gcgttgagac
     6421 cgtttctggc ggcatcgctg tcgaggtcgc tcatgtcggc acccgcactg aacgcatcgc
     6481 cctcgcccgt cgtgacgacc gcccgcacgg tgtcgtccct gctggcctcg ccgagcgcgg
     6541 cgagatactc ggcgagcagc gtgccggtca acccgttgcg ctgctgcggc cggttgagcc
     6601 ggcagaccac gaccccgccg tgtcgctcaa ccacgacttc ggtcatcgtt caggacaggt
     6661 ccggcagctt gcctgcactg accgtcgact gcaattcgac atattcggac agcccctcgg
     6721 atccgaactc gcggccgatg ccggattgct tgaatccgcc gaacggacaa ctggtgtcga
     6781 gggtgtacat gttgatgccg taggtgcccg cgcggatctc ggcggcgatc tccaaaccgt
     6841 gcgcgatgtc ggcggtccac accgacccgg ccagcccgta gtcgctgtcg ttggcgatcc
     6901 ggatggcgtc gcgctcgtcc gagtacttga ggacggtcag caccggaccg aagatctcct
     6961 cgcgggcgat ccgcatttcg ttgttcgcat ccgcgaacag cgtcggctgg acgtaccagc
     7021 cgcgttcgcg cggtttatcc gtgccgccca gcacaattcg cgcaccctcg tcgacaccgg
     7081 tctcgatgta gccctgcacg cgatcctgct gacgttcggc gacgagcggt ccgatgtcgg
     7141 tggcttcgtc ggcgggatcg cccacctgca ggctcgacat catctccgcg agcgcgtcga
     7201 caacctcatc gtggcgccgg tcgctgacca gaatccgggt ctgcgcaacg cacgcctgcc
     7261 cgttgttcat caggctggcc atcttcaggt ggttgacggt gcgatcgatg tcggcgtcgt
     7321 cgaggatgat cgccgcggat ttgcctccca attccaggct cacccgcttg agttgttcac
     7381 cgcacagcgc ggcgatgcgc ctgcccgttg acgaggaacc ggtgaacgat atcttgtcca
     7441 cgcccggatg gcggaccagg gtttcgcctg tttcgcggcc gccgggaatg atcgacacca
     7501 cgccctccgg aaggtcgatc tcgtccagca tctcggccaa ccacattgcg tccaagggtg
     7561 tttcgggcgc tggcttgacc acgacggtgc agcccgcgat cagcgcggga atgagcttcg
     7621 gcatgatcag gaactgcggg acgttccacg gcacgatcgc accgacgacg ccgacggggg
     7681 ctcgtcgcac gtgcacctcg ccgaacaggc cctgccgccg ctcggcccac gggaattcgc
     7741 gtgccgtcgc gaggttcagg tgcatctggg ccaccgcccc ggtggcctgg ccgagcctgc
     7801 tgaagctgcg cggtgagccc atttcggcgg tgatgagatc ggccatctcg tcggtttggc
     7861 cgctgtacag cgccgccagt ttctcgatct tttccatccg ctcggccggt gtgaggcgcg
     7921 gccatggtcc gtcgtcgaac gccgtcctgg cggccgccac cgccttgtca acgtcctcgg
     7981 gcgacgcttc aggcgtctcg ccaatcggtt cttcggtgtg cggcgagatc accgaaagcc
     8041 gctgtgcggt ggccggtgac tgccattgac cgccgatgta aagcttgtcg tagacaaggg
     8101 atctcacgcg atggcctcct cgtaggactg ccgcgcccag gcgcggaact cggtgatcgc
     8161 gccttcgccg cgggccagct gcggacggtc gcgatagatc ttgttctccc agatcgggat
     8221 gtcctgttcg atctgcttgc agaaatcgcg ggcgaaacca agacccatcc gtgtcatctc
     8281 gccggtctcc gggtcggtct ggacgaagaa cgtgtaacgg gcgtcgacga actcgccgtc
     8341 gatcggggtg acggtcagca gcgtgcacga ccggccaagc ccccggtgcc gcaccacgtc
     8401 gagtcccatg ccgaacagct cggagtcgac ggctcccgac accggcccgc gcggcgtttg
     8461 gaacgtcacc tcagcgatcg cccgtagccg gtgcccgtcg gtttccagat ccaggtcgcc
     8521 gaacccggac accccgtgca ccgtcgcgaa atgcgcgatg tcaacggtgt tttcgaacac
     8581 ctcctgcgga tgggaccgga accgccagcg ggcggaatcg ggcgcatgga acgtgaacgc
     8641 cgcgtcctcg ctctcaggga tcttgggcaa ttcccactcc ggttcggtcg ccgcttgcga
     8701 gtgccagacg aagatggcac ctgcccgctc gaccgtcggc catgcccgta gtcgcgcggt
     8761 gcggttgacg gtcttcgcgt acgggatttc gacgttcgat ccggcgccgt cgaagcgcca
     8821 gccgtgaaac gggcagacga tgcagtcgtc ggcgaccgtg ccgccgaccg cgatgtcggc
     8881 accgagatgc gggcagtacg cgtcgagcac gtacggcgtt cttgaggcgc cgcgataaca
     8941 gatcagccgt cggccgaagt agtgcagacc caccacctgt cccgcgtcga catctctgct
     9001 atacgcgacc tgaaaccagc ccttggggta ggcgtcgaac gggaagcgct tgccgatgtc
     9061 acgcatcacg gcgtcaccgc ccgtctggac cgctaaagtt gctattgctg taaacatagc
     9121 aaaaaaggca ggtgccgtgg acgaccgcga ctggcgtaac tatccgttcg atctcgtgcc
     9181 gggggacgca gcgctgtcct ttccggaggc cgagggcaat caccccgatt gcgagtccga
     9241 tacctggttc atcgccggtg agctgtccgc ggacagcggc cgtcgattcg cgtttctgac
     9301 gatcttcaac aagaacaggc cgggcgggtc catcgtggcg gacttctata cgttcgccct
     9361 gttcgatctg gacaacggcg gctacgggac gtacaccgac tacgacatgc cgccggcgaa
     9421 catggcaccg ggggcgcggc cgaagctgtc cgtcgcgacc ggccacctgg acatgaccta
     9481 cgacagcggc gcgggccaag ccgtgtggcg ggcgtgccgc gacgagcagc acctgttggt
     9541 gccctacacc tatgacgtca gcctcgtcgg caccgaccag ttcggacacg cgatgcgtct
     9601 ggacctgcac gtgactccgt cgcgcgcccc tgtcccgttg ggcggctcgg cccataacgg
     9661 caagatcacc tgcttcggcc aggaggacac gtactcctac ttccagaccc ggatggtcat
     9721 gacgggcacc gtgcgctggg gagcagcgga ggaatcggtc accggggaga cgggccacgt
     9781 cgaccggcaa tggttcccgc tggttgccaa cagtggcggg gcgaccggtg atatccgctc
     9841 acgggcgcac gaatggcgca cgatcaacct cgacaacggc gtcgacctgt ccatctggcg
     9901 gcagttcgat cgcactgaca acaatgcgct gcaaccgttt tcgggtgcga cgacgagttc
     9961 gcccgacgcc gatcccgaat atgccgacga catcgaggtg gtcgtcgaca gctatgtgcg
    10021 ctggcccgag tcgatccgca cgttggtgcc gccgcccgcc gcgctgcggt atatgcctga
    10081 ccggcataga ctgacgtctc gcaagctcga cctggacctg acgggagagc cgttggtcgc
    10141 ggcaccggcg catgccttac cgctcgagta catggagggg ccgtaccggt atcgcggaac
    10201 gatgcgcgga aaaccggtga gcggcttcgc gttctacgag cgctcgctcg cgctgtatcg
    10261 ggattgggag ttggccgaga tcgccgacgc ccacgtttga tggcggtgct gcagcccagg
    10321 ttcgttgcga ccgaacacca aacgctcccg cgccccggat tccaaattcg cttgcaggcc
    10381 ggcgaccagt gcgtagtcct catcgcgcac cgtggcgtgc gcgtagtcga acaccgccgc
    10441 ggcacccgcg gccaccgatt cgtcggacag atccagtggc gtggaattct ggtgcacggt
    10501 gatcgaccgt cccggcacat cggcgggata gacgcggaac agttcgccat tggcgatcgt
    10561 gcacgacaac acgatgttcg ggaacagcgc gtagatcacc accatgttct gcagcggatc
    10621 ccactgctcc tccggcacgt cctcgaggtc gagaattccg ttgagaggga agacaagtcg
    10681 gtggtgcacg ccgaacgaat cgaacaccgt gcagttgctg cgcgcgatgg tcgcgaacgt
    10741 ggccttgtgc acggtcgcga aatggtaatt ctcggcgaat gtgtcgatcg cgagcttcca
    10801 gttgatcgga cagtcgagca ccttctcacc catcggcgac cattgcccga tgccccacga
    10861 ctccagttcg ccggcgagcg gtccgaggaa cgccggaatg tcgagcgtcg catcgcggtc
    10921 cagcgatatc cataggaagc ctgcacactc ggccgccggc agctcggtca gtcgcgccgg
    10981 gccggagcgc gtttcgggga atccttcctt gccgggtacg ccgacgagat tgccgtccag
    11041 gtcgtaggtc cacgagtggt acggacatgc aagccggcgc gcggctccgc agccgtcggc
    11101 gatgcgtgac tggcggtgca ggcagatgtt gtcgaacgcg cgcacagcgt tgtcggcgct
    11161 gcgggtcaac agaatcgagc ggcccatcac ggtcttggtg cagtacgtgc cgggctgagg
    11221 gagttcggag gaatagccga cgagttgtgg gctcgccatc agcattgccg tgtcgagcgc
    11281 gtggcggtcg ttcgacgtat aggtatccgc ggcgacggtg tgctccgcca gcgtgaggtc
    11341 ggtggtcttg tcgcgtgcga gcttgaccgc gcggcgggtg aggtcgatga gctggtcgcg
    11401 atgcatgaaa ccctattctt gttgttttta taacaattgc caaccgtcgg cgaaaagtga
    11461 ctggtgatgc tcgtgttgcg cgtatcgtcc gctaaatccg gggttgggat gacgtgaagg
    11521 ggaagccatg acgttcaggg gagggatgtc cagcggctgg gtgaaaaggt tagcgatcgt
    11581 cgcgttcgcg accgccgtgg tacccgggtt ggtcagcctc gcaggtggaa ccgtgaccgc
    11641 tggggcgttc tccagccccg ggctgccgat cgagtacctc gacgtgccat cccagggcat
    11701 gggccgcaac gtcaaggtcg aattcatgag cggcggtccc accgcccccg cgttgtatct
    11761 cttggacagc atggaagccg gtgacgactt caacggctgg gacatcaaca cccaggcgtt
    11821 cgactggtac aacgggtcgg ggctgtcggt ggtcatgccg gtcggcggca agtcgagctt
    11881 ctatagcgac tggtacgggc ccgctgtcgg caacgccggc acgtacacct acaagtggga
    11941 aaccttcctc acccaggaac tgcccgcctg gctggcggcc aacaagggca cgccgcagac
    12001 cggcaatgcg atagtgggat tctcgatggg cggttcgtcg gcgctggtgc tggccgccta
    12061 ccacccgcaa cagttcatct acgccggatc catatcgggc tttttgaacc tgtcggcgga
    12121 ccccgggcag gttggcatcg cgatgatgtg gaacggcggc ttcagccgcg aagcgatgtg
    12181 ggggccgccg ggcgacccgg cctgggcccg caacgatccc accctgcagg caggcaggct
    12241 cgcagccaac ggcacccgac tgtgggtcta ctgcggcagc ggaacaccga ctgatccagc
    12301 cttggcaagc ccggaggcgc ccatcggcgg actcggtttc ctcgagggaa tggcgatcga
    12361 ctccaac
//
LOCUS       DASRJA010000747        11636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4102649,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000747 DASRJA010000000
VERSION     DASRJA010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11636
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4102649"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VFC01_33760"
     CDS             <1..1047
                     /locus_tag="VFC01_33760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016886844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-related domain-containing protein"
                     /protein_id="HZN84625.1"
                     /translation="QFRNFSYLDTQLIRLGGPNFAQLPVNRPLADVRNNHQDGYGQHA
                     IPQGRSSYFKNTLGGGCPALADEDVFRHYTEKVDGNKIRQRAKSFEDHYSQARMFWKS
                     MTPVEAKHIVAAYAFELGKVEVPEIRARVVEQLNLVDHDLAAQVADKLGVPEPAEESV
                     DDKMAASPALSQLNTASDSIATRKIAVLAADGVDVQGVDRLTRALEQQGAIVEVLAPV
                     AGGTLSGGSGGEVGIDRAITTMASVLYDAVVVPCGPESVETLSNDGYAMHFVTEAYKH
                     LKAVGAFGSGVDLLRKAGITEQLADDTDVVVSNGVVSTAAAADDLTDEFSEAFAAAVA
                     KHRAWDRETDPVPA"
     gene            complement(1350..1817)
                     /locus_tag="VFC01_33765"
     CDS             complement(1350..1817)
                     /locus_tag="VFC01_33765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZN84626.1"
                     /translation="MKLAVQRLLIHAPAEEVFEYFIDPVRFGQWMAEGATLNPEQGGT
                     VRWTHPNGDSVSGRYVEVNPPRRLVFTYGWERAEVRIPPGSTTVEIDLITQDDGTTLI
                     TLVHRGLEGGAVDAHRGGWAHYLDRLRSAAEGQPSGPDPWADRRVPTPEELAR"
     gene            complement(1814..2137)
                     /locus_tag="VFC01_33770"
     CDS             complement(1814..2137)
                     /locus_tag="VFC01_33770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN84627.1"
                     /translation="MSDPAVEDAIRALAHPGRRAMLRLVWDEERPATELADAAGLSPS
                     AASQHLKLLRDTGLITVRVDATRRLYRADLTRVAEIAAFLDDFWAAPLDRLRAAAEKS
                     RGGSS"
     gene            complement(2134..2688)
                     /locus_tag="VFC01_33775"
     CDS             complement(2134..2688)
                     /locus_tag="VFC01_33775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HZN84628.1"
                     /translation="MLNTPHPVALYRAATEHATGVIDAARPDQLGLPTPCTEWTVQQL
                     IDHLVGGTEYLLSAAEGNEPTQQADATAADYRRGTAAVMDALEQPGVMERTCISPLGF
                     EWPVSQAVAGTFMDVLIHTWDVARATGQDEKLDPELVEACTAMFLPDMPERGRAAGIV
                     GPAVEVGENASPQDRLLAAMGRHP"
     gene            2754..3206
                     /locus_tag="VFC01_33780"
     CDS             2754..3206
                     /locus_tag="VFC01_33780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN84629.1"
                     /translation="MAPVDVEVETVIHRPRSIVAAYAADPDNATAWYVNIKGVRWKTP
                     RPAALGSQFEFSAAFMGRTLTYTYEVVEFVADQRFVMRTTEGPFPMETTYEWFDVDQH
                     STRMKLRNRGEPSGFSSIAAPMMALAMKRANTKDVKRLKAILEQEQLT"
     gene            complement(3203..3679)
                     /locus_tag="VFC01_33785"
     CDS             complement(3203..3679)
                     /locus_tag="VFC01_33785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZN84630.1"
                     /translation="MADIGVSTPKAPLDHVSKSIIDALQQDGRRSYSAIAEAVGISEG
                     AVRQRVQRLMNAGVMQIVAVTDPTELGFGREAMIGICCSGDSIRVAERLTQVDAIDYV
                     VLTAGRFDIVVEVVCEDDTHLLDILNTHIRSQPGVYLAETLVYLKTLKQQYNWGTR"
     gene            complement(3740..4204)
                     /locus_tag="VFC01_33790"
     CDS             complement(3740..4204)
                     /locus_tag="VFC01_33790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84631.1"
                     /translation="MIIERQSVVDAPADQVWARVVTPEGINDEVRPWMTMSMPRGAES
                     LTVDNVPIGVPIGRAWLRLFGVLPFDYDRLCIAELDPGRGFDEQSTMMSMRQWRHQRT
                     VTPDGDSKAVVRDRITFQLRALLRPATPIVAAGLRALFGHRHRRLQRYFRNR"
     gene            complement(4259..5548)
                     /locus_tag="VFC01_33795"
     CDS             complement(4259..5548)
                     /locus_tag="VFC01_33795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HZN84632.1"
                     /translation="MAIVALGVFIVAYTLIATERIHKVKVALGGAAVVLALGIVDSQD
                     VFYSHETGIDWDVIFLLLGTMVIVGVLRQTGCFEYAAIWAAKRAGGSPLKVMILLVLI
                     TATASAALPNVTIVLLMAPVTLLVCDRLDINPIPFLIAEVLASNIGGVSTLIGDPPNI
                     IIGSRANLSFNDFLVHLAPVAVIALVAFVATLPLLFRGTFGVHADRVAEVMSLNEREA
                     IRDVRLLAKSGLVLVGVIAALVASSAIHVEPSIVALIGAGVLVIISGLESVHYLASVE
                     WETLLFFAGLFILVGSLVKTGVIADLARLAGDVTGGDPLFATMLILVVSAVLSGLIDN
                     IPYVATMTPVVADIATTIPNPGHVQAMWWALAIGAGFGGNMTAVGASANVVMVGIAGR
                     EGYPIGFWQFTRKGAVVTALTVAVAAPYLWLRYFVFG"
     gene            complement(5574..6017)
                     /locus_tag="VFC01_33800"
     CDS             complement(5574..6017)
                     /locus_tag="VFC01_33800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZN84633.1"
                     /translation="MRAEQIAEEFPEVALDSDALEAVRLLATHRLPGLVVTDTAGTPV
                     TILPASQVVRLLVPHYVQDDPSLAGVLSESMADRVADKLGGKTVKEVLPKPPPEMTMV
                     KADDTIVEVAAAMARNRSPLVAVMGRDGLVGVITATRLLEVALAH"
     gene            6207..7478
                     /gene="nhaA"
                     /locus_tag="VFC01_33805"
     CDS             6207..7478
                     /gene="nhaA"
                     /locus_tag="VFC01_33805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HZN84634.1"
                     /translation="MPLRLLTRGSWSETQRFSQILRKETVGGMLLLAAAGAALVWANS
                     PWAHAYHAMSEFAIGLESLHLRLSVSAWAADGLLAIFFFVVGVELKREFVAGDLRDPR
                     RAALPVAAAIGGMIVPAGIFIAINLAAGRPENLAGWAVPIATDIAFALAVLAVLSTHL
                     PTALRTFLLTLAVVDDLLAITVIAAFYTDHLSLGPLALALIPIALFGFAVQRGFGRWW
                     VLLPLAATAWALVHASGVHATVAGVLLGFTVPVLGRHASAEDFEHALRPVSAGFAVPV
                     FAFFAAGVTVGGWSGLGDSLLHPITDGVIAGLVIGKPLGVWLTAFLLAKFTHASLDDD
                     LAWRDVLGVSLLAGIGFTVSLLIGELAFGHGTPAGDDVKIGVLVGSVIAGLLASLVLL
                     SRNAAYRRIHEFETADADHDGVPDVYESQQD"
     gene            7507..9420
                     /gene="dxs"
                     /locus_tag="VFC01_33810"
     CDS             7507..9420
                     /gene="dxs"
                     /locus_tag="VFC01_33810"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZN84635.1"
                     /translation="MLEQIRGPADLQHLSQSQLSDLAQEIREFLIHKVAATGGHLGPN
                     LGVVELTLALHRVFDSPHDPIIFDTGHQAYVHKMLTGRSHDFDTLRKKGGLSGYPSRS
                     ESEHDWVESSHASSALSYADGLAKAFKLAGHRNRHVVAVVGDGALTGGMCWEALNNVA
                     AARRPVVIVVNDNGRSYAPTIGGFAEHLAGLRLQPGYEKVLDEGRKVVRGVPLIGELC
                     YQCLHSVKAGIKDALSPQVMFTDLGLKYVGPIDGHDEHAVETALRQARGFNAPVIVHV
                     VTRKGMGYPPAENDEADQMHSCGVIDPETGLATSVPGPGWTSAFSDALIGYAERRRDV
                     VAITAAMPGPTGLTKFRQRFPDRFFDVGIAEQHAMTSAAGLAMGGLHPVVAIYSTFLN
                     RAFDQIMMDVALHKLPVTMVLDRSGITGPDGASHHGMWDLSMLGIVPGMRVAAPRDGT
                     RLHEELGEALDVNDGPTALRFPKGDVGEDIPALERRGGVDVLAVPAEGLSEDVLLVAV
                     GPFAAMAVAAAERLRNQGIGVTVVDPRWVLPVPDALSELARAHKLVVTVEDNGVNGGV
                     GSAVSAALRRVEIDVPCRDVGLPQEFCAQASRGEVLADVGLTDRNIARQITGWVAAQG
                     ATVHEPEVSERLD"
     gene            complement(9501..10754)
                     /locus_tag="VFC01_33815"
     CDS             complement(9501..10754)
                     /locus_tag="VFC01_33815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HZN84636.1"
                     /translation="MAEPDEAAVEPAEPEATPLLAPREGVPRLSVSQSEIARAAELLA
                     SGSGPFAVDAERASGFRYSNRAYLVQIRRAGAGTVLIDPVNHGADPVATLAPVAEVLV
                     TDEWVLHAADQDLPCLAEIGLRPPKLYDTELAGRLAGYDRVNLAAMVQRLLGLGLMKG
                     HGAADWSKRPLPHDWLNYAALDVEVLLDLRDAIADVLQEQGKTDWAAQEFEYARTVEA
                     TPTRRDRWRRTSGIHKVRNPRALAAVRELWLTRDHIARRRDIAPGRILPDSAIVNAAT
                     VDPDTVEKLTELPIFGGNRQRRSAQVWLDALARARTAEPPEAQEPPNGPPPASRWARR
                     KPEAAVRLEAARAGLTELSQQVSVPTENLLSPDVVRRLCWDWQPAEDVAAAVETFLRD
                     AGARPWQRELTVPVLTAALTAAPES"
     gene            complement(10757..11338)
                     /locus_tag="VFC01_33820"
     CDS             complement(10757..11338)
                     /locus_tag="VFC01_33820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015355345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HZN84637.1"
                     /translation="MTTAEPAQFREAVAAMNATTVRPEIELGPIRPPQRLAPFSYALG
                     AEVRHPETAIVPERSEGDAFGRLILLHDPDGAEAWDGTMRLVAYIQADLDSTEAVDPL
                     LPEVAWSWLVEALEQRAEHVTALGGTVTATTSVRYGDISGPPRAHQLELRASWTATTL
                     ELGPHVQAFCEVLEHAAGLPPAGVTDLSSRTRA"
     gene            11430..>11636
                     /locus_tag="VFC01_33825"
     CDS             11430..>11636
                     /locus_tag="VFC01_33825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84638.1"
                     /translation="MPERVADAIEEIARPNRSDQQEKRQIQVDPALRRPRLIRKAPAW
                     VVAVVVLAPAVAAGVAVFWALGLPV"
BASE COUNT         1957 a         3854 c         3847 g         1978 t
ORIGIN      
        1 cagttccgca acttctcgta cctggatacc cagctgatcc ggctgggtgg gccgaacttc
       61 gcccagctgc ccgtcaaccg gccgctggcc gacgtccgca acaaccacca ggacggctac
      121 ggacagcacg caataccgca ggggcggtca agctatttca agaacacgct cggcggcggc
      181 tgcccggcac tggccgacga ggacgtattc cggcactaca ccgagaaggt cgacggcaac
      241 aagatccgcc agcgcgccaa gagctttgag gaccactaca gccaggcgcg gatgttctgg
      301 aagagcatga cgccggtgga ggccaaacac atcgtcgctg catacgcatt tgaactcggc
      361 aaggtcgagg tgcccgagat ccgcgcccgc gtcgtcgagc agctcaacct cgtcgaccac
      421 gacctggcgg cgcaggtcgc ggacaagctt ggggtaccag aacccgcgga agagtcggtc
      481 gacgacaaga tggccgcctc accggcgctg tcacagctga acaccgcatc cgacagcatc
      541 gccacccgca agatcgcggt gctcgccgct gacggggtcg acgtgcaggg cgtcgaccgg
      601 ctgacccgtg cgctggaaca acagggcgcg attgtggagg tattggctcc tgtcgccgga
      661 ggcacgttgt ccggcggatc aggcggtgaa gtaggcatcg accgcgcgat caccaccatg
      721 gcgtcggtgc tgtacgacgc ggtggtcgtg ccgtgtggcc ccgagtcggt cgagacgctg
      781 agcaacgatg gctatgcgat gcacttcgtc accgaggctt acaagcatct aaaggccgtc
      841 ggcgcatttg gttccggtgt cgatctcctg cgcaaagcgg gcatcaccga gcagctcgcc
      901 gacgacaccg acgtcgtcgt gtcgaatggg gtcgtgtcga ccgcggcggc cgccgacgac
      961 ctcaccgatg agttctccga ggcgttcgcc gcggctgtgg ccaaacaccg tgcatgggac
     1021 cgggagaccg acccggtacc agcctgacga agcatagggc ctccgccaag taatcagccc
     1081 aaaggtcttg ccgcacaatg ctgatcgtcg tccactgctt gaagcgccgc cccgcggggg
     1141 tgaacgaccc tgcgcgaccg gcggcaacca actcgtccac ccgcgcctcg gccaacttga
     1201 cgacaagttc accgctgcgc cggtcaagac aggcgaagaa ctggccatcc cagcgcaaac
     1261 agggcagccc atcgtggtgg agcgggtgac gcgtgcatcg gcgcagagac cgtcagcgag
     1321 ctcccagaac tgttcctcag gcgctgacat catcgggcca actcttccgg cgtgggcact
     1381 cggcggtcgg cccacggatc gggtccggat ggttggcctt cggcggcgct ccgcaggcgg
     1441 tccagatagt gggcccatcc gccgcggtgc gcgtccaccg cgccgccttc gagaccccga
     1501 tgcaccagcg tgatcagcgt tgtgccgtcg tcttgagtga tcaggtcgat ctcgaccgtc
     1561 gtcgatcctg gcggaatccg aacttcggca cgctcccagc cgtaggtgaa taccaggcga
     1621 cggggcggat tgacctcgac atagcgaccg gaaacgctgt ccccgttggg atgtgtccag
     1681 cgcactgtgc cgccctgctc cggattcagt gtggcgcctt cggccatcca ctggccgaaa
     1741 cgcacgggat cgatgaagta ctcgaacact tcctcagcgg gcgcgtggat gagcaagcgc
     1801 tggacggcca gtttcacgac gagccgccac gtgatttctc ggcggccgcg cgtaggcggt
     1861 cgagcggagc ggcccagaag tcgtcgagaa acgccgcgat ttcggcgacc cgggtgagat
     1921 cggctcggta cagccgccgg gtggcatcga cgcgcaccgt gatcaatccc gtgtcgcgca
     1981 gcaacttgag gtgttggctc gccgccgacg gagaaagtcc tgcggcgtca gcgagttcgg
     2041 tcgcgggccg ctcttcatcc cacaccaggc gcagcatcgc gcgtcggccg ggatgtgcca
     2101 gcgcgcggat cgcgtcttcg actgctgggt cgctcacgga tggcgtccca tcgccgccag
     2161 gagccggtct tgtggagagg cgttctcgcc gacttccact gctggaccaa ctattccggc
     2221 cgcgcgaccc cgttcgggca tgtccggcaa aaacatcgcc gtacacgcct ccaccagttc
     2281 agggtcgagc ttctcgtcct ggccggtggc ccgtgcgacg tcccacgtgt ggatcaatac
     2341 atccatgaac gttcctgcga ccgcctgcga gaccggccat tcaaagccaa gtggtgagat
     2401 gcaggttcgt tccattacgc cgggctgctc gagcgcgtcc attacggctg cggtaccgcg
     2461 ccggtaatcg gcggccgtcg cgtcggcctg ctgcgtgggc tcgttgcctt ctgcggcgga
     2521 cagcaagtac tccgtcccgc caacgaggtg gtcgatcagc tgttggacgg tccattcggt
     2581 gcaaggcgtc ggcaatccga gctgatcggg ccgtgccgcg tcgatgacgc cggtcgcgtg
     2641 ttcggtggcg gcacggtaaa gcgctactgg gtgcggagta tttagcatgc tctaaattta
     2701 gaatgcactg aatattcgtg tcaacgggtg tcgggctgtg gtaagcgatt cgtatggccc
     2761 cggtcgatgt cgaagtcgag acagtcatcc accggccacg ctcgatcgtg gccgcctatg
     2821 ccgccgatcc cgacaacgcc acggcttggt acgtcaacat caagggcgtg cggtggaaga
     2881 cgcctcgtcc ggcggcgctg ggttcgcagt tcgaattctc tgccgcattc atgggccgca
     2941 cactgacgta tacctatgag gtcgtggagt tcgtggccga ccagcgcttc gtcatgcgca
     3001 cgaccgaagg gccgtttccg atggagacca cctacgagtg gttcgacgtc gaccagcatt
     3061 cgacccggat gaagctgcgt aatcgcggcg agccatcggg gttctcgagc atcgccgccc
     3121 cgatgatggc gctcgcgatg aagcgcgcca acacgaaaga cgtcaagcgg ctcaaggcca
     3181 tcctggagca agaacagtta acttagcggg taccccaatt gtattgctgc ttaagtgttt
     3241 tcaggtagac tagagtttcc gcgaggtaga cgcccggctg cgaccggatg tgggtgttga
     3301 gaatgtccag cagatgcgtg tcgtcctcgc acaccacctc gaccacgatg tcgaacctgc
     3361 cagccgtgag cacgacgtag tcgatcgcgt cgacctgcgt gagcctttcg gcgacccgga
     3421 tgctgtcgcc tgagcagcag attccaatca tcgcctcgcg cccgaagccg agttctgtgg
     3481 ggtcggtgac ggcaacgatt tgcatcacac cggcgttcat caatcgttgc acccgctggc
     3541 ggaccgcccc ctcggaaatg ccgaccgcct ccgcgatcgc cgagtacgac cggcgcccgt
     3601 cttgctgcag cgcgtcgatg atggacttgg agacgtggtc cagtggcgcc tttggagtcg
     3661 aaacaccgat gtcagccatg ccgtcgattc tgcaccgcgg ccgaccggtg cgtgcacatg
     3721 tcgctttgct cgcgtgtcgc tagcggttcc tgaaataccg ctgcagtcgc cggtgccggt
     3781 gcccgaacag cgcgcgcaac cccgctgcga cgatcggtgt ggctggacgc aacagcgcgc
     3841 gcaactgaaa tgtgatgcga tcgcggacaa cggctttgga atctccgtcg ggcgtcacgg
     3901 tgcgttggtg ccgccactga cgcatgctca tcatcgtgga ttgctcgtcg aaaccgcgcc
     3961 ctggatccag ttcggcgatg cagagtcggt cgtagtcgaa cggcaggacg ccgaacagtc
     4021 gcagccaggc gcgcccgatc ggtacgccga tcggcacgtt gtcgacggtc agcgattcgg
     4081 ccccgcgcgg catcgacatg gtcatccagg gtcgaacttc gtcgttgatg ccttcgggcg
     4141 tgaccacccg cgcccacacc tggtcggccg gagcatcgac gacgctctgc cgttcgatga
     4201 tcatgccgat tcacggtatc cgacgggacc gagcgatggt gggctacgtc gcggccacct
     4261 agccgaagac gaaatagcgc aaccacagat acggggcggc gaccgcgacc gtcagtgccg
     4321 tcacaacggc ccctttgcga gtgaattgcc agaacccgat cggatatccc tcgcgtccgg
     4381 cgattccgac catcacgacg tttgcgctcg ccccgaccgc agtcatgttt ccgccgaacc
     4441 cggctccgat ggccagcgcc caccacatgg cctgcacatg gccaggattg ggaatggtcg
     4501 tggcgatgtc ggcaaccacc ggggtcatgg tcgcgacgta cgggatgttg tcgatcaggc
     4561 ccgaaagtac cgctgagaca acgagtatca acatagtcgc gaacagcgga tcgccgccag
     4621 tgacgtcgcc tgccagcctc gccagatcag cgatcacacc cgtcttcacc agcgatccga
     4681 ccaggatgaa caaaccggcg aagaacagca acgtctccca ttccacactg gccagatagt
     4741 ggacactttc gagaccggag atgatcacca acacgccggc gccgatgagc gccacaatgg
     4801 atggctcgac atgaatagcc gaactcgcga ccaacgcggc gatcacgccg accagcacca
     4861 gacctgactt cgcgagaagc cggacatcgc ggattgcctc gcgttcgttg agggacatca
     4921 cctcggcaac ccggtcagcg tggacaccga acgttccgcg gaacagcagc ggcagcgtcg
     4981 ccacaaatgc caccagcgcg atgaccgcca cgggtgccag atgcaccaga aagtcgttga
     5041 acgacaagtt cgctcggctc ccgatgatga tgttcggtgg atcgccgatc aacgtcgata
     5101 ccccgccgat gttggacgcc agcacctcgg cgatcaggaa cgggatcggg ttgatgtcga
     5161 gacgatcaca caccagcagc gtcacgggcg ccatcagcag cacgatggtc acattgggca
     5221 gcgccgccga agctgtcgcg gtgatgagga cgagaagaat catcacctta agcggagagc
     5281 cgccagcccg cttggccgcc cagatcgcgg cgtactcgaa gcagcccgtc tgccgcagca
     5341 ctccgacgat gaccatcgtg ccgagcagca gaaagattac gtcccagtcg attccggtct
     5401 cgtgcgagta gaacacgtcc tgcgagtcga cgatccccag cgccagcacc accgctgcgc
     5461 cgcccagcgc gaccttgacc ttgtgtatgc gctcggtcgc gatcaaggtg tacgcgacga
     5521 tgaaaacgcc tagcgcgacg atcgccatgg cgccaccccc ggactatcgg ggctcagtgc
     5581 gcgagagcaa cctcgagaag gcgtgtagca gtgatgacgc cgacgagtcc atcgcggccc
     5641 atcaccgcaa ccagcggcga ccgattgcgc gccatcgcgg cggccacttc gacgatggtg
     5701 tcgtccgcct tcaccatcgt catctcgggc ggcggcttgg gcagcacttc tttgactgtc
     5761 ttgccgccca gcttgtccgc gacgcggtcg gccatcgatt cgctcaatac ccccgcgagc
     5821 gacggatcgt cctggacgta atgcggcacg agcaggcgca cgacctgcga agcaggcagt
     5881 atcgtcaccg gagtcccagc ggtgtcggtc acgaccagcc ccggcagccg atgggtggcc
     5941 agcagccgaa ccgcctcgag cgcatcggag tccaacgcca cttccggaaa ctcctcggcg
     6001 atctgctccg ccctcacacg tcaacgctac gacgccgcac ggcaaatgag cactgatcca
     6061 tatggttcct tgatgcagcg ccatgtggta ctcccgcggc cgtcgtccaa gtcgcaacac
     6121 gcggtgcgac tgccgaccag acttcccggc gctccacacg taaagatgcc gtaaacggtg
     6181 aagctgggca acactaatat cggccaatgc ctcttcgact gctgacacgt gggtcgtggt
     6241 cggagacgca acgcttttcc cagatccttc gcaaagagac cgtcggcggc atgctgctgt
     6301 tggctgccgc tggtgccgcg ctggtgtggg cgaactcgcc gtgggcacac gcgtaccacg
     6361 cgatgtcgga gttcgcgatc ggtctcgaat cgctgcatct gaggctgagt gtgtcggcgt
     6421 gggcagctga cgggctgctg gcgatcttct tcttcgtcgt cggggtggag ttaaagcgcg
     6481 agttcgtcgc cggcgatctg cgcgacccac gccgtgccgc gctgccagtc gcggcggcca
     6541 tcggcggcat gatcgtcccc gccgggatct tcatcgcgat caatctggcg gcgggccgtc
     6601 ccgagaacct cgccgggtgg gctgttccga tcgcgacgga catcgccttc gcgctggcgg
     6661 tgctggccgt cctgtcgacg catctgccca ccgcgctgcg cacgtttctg ttgacgctgg
     6721 cggtggtcga cgacttgctc gccatcaccg tcatcgccgc cttctacacg gatcacttat
     6781 cgctggggcc gcttgcgctg gctctcatac ccatcgcact gttcggcttc gcagtacagc
     6841 gcgggttcgg acgctggtgg gtgctcttgc cgctggccgc cacggcctgg gccctggtcc
     6901 atgccagcgg tgtgcatgcc accgtcgcgg gtgtgttgct tggcttcaca gtgccggtgc
     6961 tcgggcggca cgcgtcggcc gaggacttcg aacacgcctt gcggccggtt tccgcggggt
     7021 tcgcggtgcc cgtgttcgcg ttcttcgccg cgggtgtgac ggtcggcggc tggtcgggcc
     7081 ttggcgactc gctgttgcat ccgatcaccg acggcgtcat cgcgggcctg gtgatcggca
     7141 agcctctcgg cgtgtggctg acggcgtttc tgctggcgaa gttcactcac gccagcctcg
     7201 acgacgacct ggcgtggcgc gacgtgctcg gcgtgtcact gctcgccggc atcggcttca
     7261 cagtttcgtt attaattggt gaacttgcgt tcggacacgg caccccggcg ggcgatgacg
     7321 tcaagatcgg tgtcctcgtc ggctccgtta tcgcaggtct actggcgtcg cttgtgttgt
     7381 tgagccgcaa cgctgcgtat cggcgaatcc atgaattcga aactgcggac gcggaccacg
     7441 acggtgtgcc cgatgtgtac gagtctcaac aggactgagc cacaggcgtc tgcgtacact
     7501 gacctaatgc ttgaacagat ccgcggtccc gccgatctgc agcacctttc tcagtcgcag
     7561 ctgagcgatc tggcacagga aatccgcgaa ttcctgatcc ataaggtcgc tgcaaccggc
     7621 ggtcatctcg gcccgaacct cggtgtggtg gagctcacgc tggcgctgca cagggtgttc
     7681 gactcgccgc acgacccgat catcttcgac accggccatc aggcctacgt gcacaagatg
     7741 ctgacggggc gcagccacga cttcgacacg ctgcgcaaga agggcggcct gtcgggctat
     7801 ccgtcgcgca gcgagagcga gcacgactgg gtggagtcca gccacgccag ttcggcgctg
     7861 tcgtatgcag acggcctggc caaggcgttc aagctggccg ggcatcgcaa ccggcacgtg
     7921 gtcgccgtcg tcggcgacgg ggcgttgacg ggcggcatgt gctgggaggc gctgaacaac
     7981 gtcgccgcgg cgcgcagacc ggtcgtgatc gtggtcaacg acaatggccg cagctacgcg
     8041 ccgaccatcg gcggattcgc cgagcatctg gctgggctgc ggttgcagcc gggctacgag
     8101 aaggtgctcg acgagggccg caaggtggtc cgcggcgtgc cgctcatcgg cgagctgtgc
     8161 taccagtgcc tgcacagcgt caaggcgggc atcaaggacg cgctgtcgcc gcaggtgatg
     8221 ttcaccgatc tcggcctgaa gtacgtcggg ccgatcgacg gccacgatga gcacgcggtg
     8281 gagaccgcgc tgcgccaggc gcggggcttc aatgcgccag tgatcgtgca cgtcgtcacc
     8341 cgcaagggca tgggctaccc gccggccgag aacgacgagg ccgaccagat gcactcatgc
     8401 ggcgtgatcg accccgagac ggggctcgcg acgtcggtgc cgggaccggg ctggacgtcg
     8461 gcgttctccg acgcgctgat cggctatgcc gagcggcgtc gcgacgtcgt ggcgatcacg
     8521 gctgcgatgc caggtccgac ggggctgacg aagttccgtc agcgtttccc cgacaggttc
     8581 ttcgacgtcg gcatcgccga gcagcatgcg atgacgtcgg ccgccggcct ggcgatgggt
     8641 ggcctgcatc cggtggtggc catctattcg acgttcctga accgggcgtt cgaccagatc
     8701 atgatggacg tggcgctgca caagctgccg gtgaccatgg tgctggaccg gtcaggcatc
     8761 accggccccg acggcgccag ccaccacggc atgtgggatc tgtcgatgct gggcatcgtg
     8821 ccgggcatgc gggtcgccgc accgcgcgac ggcacccggc tgcacgagga gctcggcgaa
     8881 gcgctcgacg tcaacgacgg tccgacggct ctgcgtttcc ccaaaggtga tgtgggcgaa
     8941 gacattccgg cgctcgagcg gcgtggcggg gtcgacgtgc tcgccgtccc tgccgagggc
     9001 ctgtccgagg acgtgctgct ggtcgcggtc gggccgttcg ccgcaatggc agtggccgcc
     9061 gccgagcggc tgcgcaacca gggcatcggc gtgaccgtgg tcgacccgcg ctgggtgctg
     9121 ccggtgccgg acgctctgag tgagctggcg cgcgcgcaca agctggtggt gaccgtcgag
     9181 gacaacggcg tgaatggcgg cgtcggctcg gccgtgtcgg ctgcactgcg gcgcgtcgag
     9241 atcgacgtgc cgtgccgcga cgtcgggctg ccgcaggagt tctgcgcgca ggcgtcgcgc
     9301 ggcgaggtgc tggccgatgt aggcctgacc gaccgcaaca tcgcccgcca gatcaccggc
     9361 tgggtggctg cgcagggcgc gaccgttcac gagccggaag tcagcgagcg cctcgactaa
     9421 cttctgccga gtggccagtt gtgacacgca tttcgcccga tcgcgtgaca taactggcca
     9481 ttcgaccgtc tggtgcggaa ttaggactcc ggcgccgccg tcaacgcggc ggtcaggacg
     9541 ggcaccgtca gctcgcgctg ccacggcctt gccccggcgt cgcgcagaaa ggtctcgacg
     9601 gcggccgcca catcctcggc cggctgccag tcccagcaca gtcgtcgcac cacgtcgggg
     9661 gagaggaggt tctccgtcgg caccgaaacc tgttgtgaca gttcggtcaa cccggcgcgg
     9721 gcggcctcaa gtcgaacggc cgcctcgggc ttgcggcggg cccagcgcga cgccggcggg
     9781 gggccgttcg gcggctcctg cgcctcgggt ggctccgccg tgcgggcgcg ggccagcgca
     9841 tccaaccaca cctgcgcgct gcggcgctgc cggttgccgc cgaatatcgg tagctccgtc
     9901 agcttttcga cggtatcggg gtcgacggtg gcggcgttga cgatcgcgga gtcgggcagg
     9961 atccgccccg gggcgatatc gcggcggcgg gcaatgtggt cacgcgtcag ccacaactcc
    10021 cggaccgccg ccagggcccg cgggttgcgc accttgtgaa tgcccgacgt gcgacgccac
    10081 cggtcccgcc gcgtcggtgt cgcctcgact gtgcgcgcgt actcgaattc ttgtgccgcc
    10141 caatcggttt tgccctgctc ctgcagaacg tcggcgatcg cgtcgcgcag gtcgagtagc
    10201 acctcgacat ccagcgcggc gtagttgagc cagtcgtgcg gcagcggacg cttcgaccag
    10261 tccgccgcac cgtgtccctt catcagccct agccccagca accgttgcac catcgcggcc
    10321 aggttcaccc ggtcgtagcc ggccagccgg cctgccagtt cggtgtcata cagcttcggc
    10381 ggcctgagcc cgatctcagc caggcagggc aggtcctggt cggccgcgtg cagcacccac
    10441 tcgtcggtga ccagtacctc ggcgaccgga gccagcgtcg ccaccgggtc ggcgccatgg
    10501 ttcaccgggt cgatcagaac ggtgccggct ccggcgcggc ggatctgcac cagataggcg
    10561 cgattggagt agcgaaagcc cgacgctcgc tccgcgtcga ccgcgaacgg ccccgaaccc
    10621 gacgctaaaa gctctgcggc cctggcgatt tcggactgac tcaccgagag ccgcggcacc
    10681 ccttctcgtg gtgccagcag cggcgtggcc tccggctcag ccggctcgac tgcggcctcg
    10741 tcaggctccg ccatcgtcaa gcgcgcgtgc gggagctcag gtcggtgacc cccgcaggag
    10801 gcaggcccgc cgcgtgctcg agcacctcgc agaatgcctg cacatgtggg ccgagttcca
    10861 gcgtcgtggc cgtccacgac gctcgaagct ccagttggtg ggcgcgcggc ggcccggaga
    10921 tgtccccgta gcgcaccgac gtggtcgcgg tgacggtgcc gccgagcgcg gtgacatgtt
    10981 cagcccgctg ctccagcgct tcgacgagcc agctccacgc cacttcaggc agcagcgggt
    11041 cgacggcctc ggtggagtcg agatcggcct ggatgtaggc gaccagccgc attgtgccgt
    11101 cccaggcttc ggcgccgtcc ggatcgtgca acagaatcag ccggccgaac gcgtcgccct
    11161 ctgaccgttc ggggacgatc gccgtctcgg gatggcggac ctccgcgccc agcgcgtagc
    11221 tgaacggcgc cagccgctga ggcgggcgga tcgggccgag ctcgatttcc ggtcgcacgg
    11281 tggtggcatt catcgccgcc accgcttcac ggaactgagc cggttcggcg gtggtcatgc
    11341 cgtttgacgc tagcccatct tgacaggcct gtgcgcaggc gcgccgattt ggcgggcgac
    11401 cggcgcagca gaggtgagac gatgggatta tgccggaacg ggttgctgat gccatcgagg
    11461 aaatcgcccg tcccaaccgc agcgaccagc aagaaaaacg tcagatccag gtggaccccg
    11521 ccctgcggcg accccggctg attcggaagg ccccggcgtg ggtggtggcg gtggtcgtcc
    11581 tcgcccccgc ggtggccgcg ggggtggcgg ttttctgggc cctcggcctc cccgtg
//
LOCUS       DASRJA010000748         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_393786,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000748 DASRJA010000000
VERSION     DASRJA010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393786"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VFC01_33830"
     CDS             <1..570
                     /locus_tag="VFC01_33830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HZN84639.1"
                     /translation="RLRAFSELLAEHRAKRDDTVLVQLATPSRERVESYVEMREDIER
                     QVGHINGEYGEVGHPIVHYIHRPMPRDELIAFYVAADVMLVTPLRDGMNLVAKEYVAC
                     RSDLGGSLVLSEFTGAAAELRQAYLANPHHLEGVKDAIEAALNQTPEEGRRRMRALRR
                     QVLAHDVDRWARSFLDALASTKDGAAKSE"
     gene            complement(557..1045)
                     /locus_tag="VFC01_33835"
     CDS             complement(557..1045)
                     /locus_tag="VFC01_33835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mammalian cell entry protein"
                     /protein_id="HZN84640.1"
                     /translation="MRWLVGSIAGLLTAAFVGLAAVGGGLYWQRVETRGEQTARDELA
                     PLAQKQIPAVFNYDYKTVERSLTDAYTMLTPDYRREFENKANSDIIPQARQREVVSQA
                     NVVGVGVMDAHRNSAAVMVYINRTVSDKSTRDQPIYDGARLRVEYKRIDGKWLINYIT
                     PI"
     gene            complement(1045..1737)
                     /locus_tag="VFC01_33840"
     CDS             complement(1045..1737)
                     /locus_tag="VFC01_33840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mammalian cell entry protein"
                     /protein_id="HZN84641.1"
                     /translation="MTSTPTRPEQAARRRASRAAGPANGKAAEVTAVRVETPVSTFTT
                     PARRSRKAAPVARRPAHRRLVAIVALAFGFFATGVLGAAVAVMIVQKNNAEATQARNQ
                     QFVDTATQTVVNMFSFKQDTIDDSVNRFYNGTSGPLRDMLSQSNNVENLKSIFRDTGG
                     SSEAVINGAALEGIDGISNNASVLVSARVTASDMNGNNRPSQPYRLRIVVHEDDAGRM
                     TAYDLKYPNGGN"
     gene            complement(1737..>3268)
                     /locus_tag="VFC01_33845"
     CDS             complement(1737..>3268)
                     /locus_tag="VFC01_33845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HZN84642.1"
                     /translation="TDDGVAAHLRLNSDIKIPANSTATVKSVSAVGEQYVDFVPPDGP
                     SQAVLRDGATIPKERTAIPQDVAELLHEADQLVSSLNNTRLQDLLRETFKAFNGSGPE
                     LARLIQSARLLIDEANASWPQTAALIDQVGPFLEAQIRSGDDIRSSADGLARFTTEVA
                     NADPQVRALLANTPSAAAEVSETFAGIRPSFPVLAANLANFGRIGVIYHRSIEQALVI
                     FPALQASLLTIGGQLPIEEGGKQDFKISINDPPPCNTGFLSPTDIRSPGDTTLRELPT
                     DLYCKVAQNDPTVVRGARNYPCQEFPGKRAPTIQLCRDPKGYVPIGNSPWRGPPVPIG
                     TPVTDPRNILPPNKFPYIPPEADPDPGYPVAPGLVPPGVQPGPGPAPHQPWPYIPPPN
                     DNGPPPPATAWIPPAPYPDAWPPPANPQPPFMFPGPYNPVVPPPGPAPAPPPPPVPAE
                     LPPAAVNPPLASGAAYGTYDQHTGVFVDPAGGKGVYAPGAANMRPQENWLDLMLYPRQ
                     T"
BASE COUNT          543 a         1044 c         1072 g          609 t
ORIGIN      
        1 cggctgcggg cattttcgga gctactggcc gagcatcgag ccaagcgcga tgacaccgtg
       61 ctggtgcagc tcgcgacgcc aagccgagaa cgcgtcgaga gctatgtcga gatgcgcgag
      121 gacatcgagc gccaggtcgg ccacatcaac ggtgaatacg gcgaagtggg tcacccgatc
      181 gtgcactaca tccatcggcc gatgccccgc gacgagctga tcgcgttcta cgtcgcggcc
      241 gacgtcatgt tggtcactcc gttgcgggac ggcatgaacc tggtcgccaa ggagtacgtg
      301 gcctgccgca gcgatctcgg cggctcgctc gtcctcagcg aattcaccgg tgccgcagcc
      361 gaactccgcc aggcatatct ggccaacccg catcacctcg agggtgtcaa ggacgcaatc
      421 gaggcggccc tgaatcagac acccgaagaa ggacggcgcc ggatgcgtgc cctgcgcagg
      481 caggttctcg cgcacgacgt cgaccgttgg gcacggtcgt tcctcgacgc gttggcgtcg
      541 acgaaagatg gcgccgctaa atcggagtga tgtagttgat cagccactta ccgtcgatgc
      601 gcttgtattc cacccgaagt cgcgcgccgt catagatggg ctggtcgcga gttgatttat
      661 cggacaccgt ccggttgatg tacaccatca ccgctgccga attgcggtgc gcatccatga
      721 ctccgacgcc gacgacgttg gcttggctca ccacttcccg ctgccgggcc tgcggaatga
      781 tgtcggaatt cgccttgttc tcgaactccc tgcggtagtc gggcgtgagc atcgtgtagg
      841 cgtcggtgag gctccgctcg acggttttgt agtcgtagtt gaacacggcc gggatctgct
      901 tctgtgcaag cggtgcgagt tcgtcgcggg cggtttgctc accacgcgtt tccactcgtt
      961 gccagtacag cccaccgccc accgcagcca ggcccacgaa cgcagcggtc aataggccag
     1021 caatgctgcc gacaagccag cgcatcaatt gccgccgttc gggtacttga ggtcgtaggc
     1081 cgtcattcgt ccggcgtcat cctcatgcac gacgatccgc agccgatacg gctgcgacgg
     1141 cctgttgttt ccgttcatgt cgctggccgt gacccgtgcc gacaccagca ccgatgcgtt
     1201 attgctgatg ccatcgatgc cttccagcgc ggccccgttg attacggcct cagaactgcc
     1261 gccggtgtcc cggaaaatcg acttgaggtt ctcgacgttg ttgctttggc tgagcatgtc
     1321 gcgcaacggc ccgctggtgc cgttgtagaa ccggttgacg ctgtcgtcga tggtgtcctg
     1381 cttgaagctg aacatgttca ccacggtctg cgtagcggtg tcgacgaact gctggttgcg
     1441 cgcctgcgtt gcttctgcgt tgttcttctg gacgatcatg accgccaccg cagccccgag
     1501 cacacccgtc gcgaaaaacc cgaacgctag cgcaacgatc gcgaccagtc ggcggtgcgc
     1561 cggccggcgt gccactggcg cagccttacg ggacctgcga gcaggtgtcg tgaaggtcga
     1621 aacaggcgtc tcaacacgca cggcggtgac ctcagcggcc tttccgttcg ctggaccggc
     1681 agccctggat gcgcggcggc gcgccgcctg ctccggacgc gttggtgtcg atgtcatcag
     1741 gtctgccttg gataaagcat tagatctagc cagttctctt gtggccgcat attagcggct
     1801 ccgggcgcgt acaccccttt tccgccggcc gggtcgacga acacaccggt gtgctgatcg
     1861 tacgtgccgt aggcggctcc gctggccagc ggcgggttca ccgcggcagg cggcaattcg
     1921 gccggcaccg gcggcggcgg cggtgccgga gccgggcccg gcggcgggac cacggggtta
     1981 tagggccccg gaaacataaa cggcggttga ggattcgccg gtggtggcca cgcatccgga
     2041 tacggcgccg gtggtatcca cgccgtcgcc ggaggcggcg gcccgttgtc gttcggaggc
     2101 gggatatacg gccacggttg atgcggcgcc ggccccgggc cgggctgcac cccaggcggc
     2161 accagacccg gcgcgacggg atatcccggg tccggatccg cctccggtgg gatgtatggg
     2221 aacttgttgg gcggcaagat attccgtgga tcggtaaccg gtgtgccgat cggcaccggc
     2281 ggcccccgcc acggactgtt cccgatcggc acatagccct tgggatcccg gcagagctga
     2341 atcgtcggcg cgcgcttacc ggggaactcc tggcacgggt aattccgcgc gccgcgcacc
     2401 acggtcggat cgttctgcgc cactttgcaa tacaggtccg tcggcagttc ccgcagcgtc
     2461 gtgtcgccag gtgatcggat atccgtaggc gacagaaaac cagtgttgca cggaggcggg
     2521 tcgttgatgg agatcttgaa gtcctgcttg ccgccttctt ctatgggcag ctggccgccg
     2581 atcgtgagga gagaagcctg cagggcagga aagatgacca gcgcctgttc gatcgacctg
     2641 tgatagatca caccgatgcg gccgaaattg gccaagttgg ccgcgagcac cgggaacgac
     2701 gggcgaatgc ccgcgaacgt ctcgctaact tcagcggccg cactcggcgt gttcgccaac
     2761 agcgccctca cctgcggatc ggcgttggcc acttcggtgg tgaagcgcgc gagaccgtcg
     2821 gccgaggacc ggatgtcgtc gccgctgcga atctgcgcct ccaggaacgg cccaacctgg
     2881 tcgatcagcg ccgcggtctg cggccagctc gcgttggcct catcgatcag caaccgcgcc
     2941 gattggatca gccgcgccag ttccggcccg gagccgttga acgctttgaa cgtctcgcgc
     3001 agcagatcct ggagccgggt gttattgagg ctgctcacca actggtcggc ctcgtgcaac
     3061 agctcggcaa catcctgcgg gatcgcggtc cgctccttcg ggatggtggc accatcacgc
     3121 agcactgcct gcgaggggcc gtcgggcgga acaaaatcca cgtattgctc gccgaccgcg
     3181 gacacgctct tgaccgtggc agtcgagttc gccgggatct tgatgtcgct gttgagccgc
     3241 agatgcgcgg caacgccgtc gtcggtca
//
LOCUS       DASRJA010000749         4323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3735120,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000749 DASRJA010000000
VERSION     DASRJA010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3735120"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFC01_33850"
     CDS             <1..325
                     /locus_tag="VFC01_33850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HZN84643.1"
                     /translation="RHALTQLGVPYQWGGTAPGVGLDCSGLTQWAYHEAGLNIPRLAQ
                     EQDVGAAVDAGSLRPGDLAVWDGHVAMIVGNGTMIEAGDPVKLSPIRTTNAGQGFQGF
                     WRPTG"
     gene            656..937
                     /locus_tag="VFC01_33855"
     CDS             656..937
                     /locus_tag="VFC01_33855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84644.1"
                     /translation="MMLPDGDRKGPLAARCAAGAGAGAGAGVTVTVTVGGGVTVTVAA
                     GADGGVDATGGADDDVDGDVAGVADGAWTEPADEAHPATVIAASATSHT"
     gene            1213..2496
                     /locus_tag="VFC01_33860"
     CDS             1213..2496
                     /locus_tag="VFC01_33860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HZN84645.1"
                     /translation="MAADIVPIRLGLTKGDLYTLWAPRWRDEGDEWEGFLGKDEDLYA
                     FESVADLVAFVRTNTDNDLADHPAWEALTQANAHTFDPPEDRQYDLVSLEELVAEKPS
                     EDTIKDLHRALAIVSSIGSVCEMPGVTKFFNGNPVLGTVGSGIDAFAGRAGRKRWAEI
                     EAVIGRGWDNAINAIDEIVTIPDVDKAASKKAEAELAEPAPEPEEVDEVEEISDTEAG
                     AEDTDAEADVEESDDLADHAKGLVLGSDDDFWLKVGIDPVRMMTSAGTYYTLRCYLDD
                     QPIFLGRNGRISVFGSERALARYLADEHDHDLSDLATYDDIRTAATDGSLEVAVSDDN
                     VYVLTGIADDLADGPDAIDRDQLELAVELLRDVGEYAEDTTVDETLDADQPLGRLVAH
                     VLDPDTVRRPSAPYAKAVEQWEALEAFVDSRLRQE"
     gene            complement(2499..2843)
                     /locus_tag="VFC01_33865"
     CDS             complement(2499..2843)
                     /locus_tag="VFC01_33865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HZN84646.1"
                     /translation="MITGAHVILYSRDADADRAFFRDVLQYPHVDAGGGWLIFKLPPG
                     EVAVHPADGPSHELYLMCDDVTATVEQLAAKGVTCGEVSDQGWGLLTSIRLPGGGDLG
                     LYEPRHPKATEL"
     gene            2900..3715
                     /locus_tag="VFC01_33870"
     CDS             2900..3715
                     /locus_tag="VFC01_33870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN84647.1"
                     /translation="MTLPITVRSVTVPGARLHYEIRGSGPLLLVIGSPMASAEFAPLA
                     GELAGNHSVVTYDPRGYAASPVEDPDAQSSVEQRADDVNAILDDLGADSADVFGSSGG
                     AVTGLALVARHPGRVRTLVAHEPPLLALLPDAADQLANTEAIIDTFHREGFEAAWRHF
                     MHNAGFDVSAGDAPPGAAEPSEDDVRQAARFFEIDMRPTTRYLPDVDTLKSSSSRVVI
                     GIGVDSGRLLTYRTSMALAELLGTAPVEFPGDHGGFMGAPKEFADVLHRVLSA"
     gene            complement(3790..>4323)
                     /locus_tag="VFC01_33875"
     CDS             complement(3790..>4323)
                     /locus_tag="VFC01_33875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZN84648.1"
                     /translation="YPPTRHLDAFEYMRGNNARFTIHAGEAFGLPSIHEAIAFCGADR
                     LGHGVRIVDDITVGADGTAQLGRLASILRDKRVPFEMCPSSNVQTGAVGSIAEHPFDR
                     LARLRFRVTVNTDNRLMSDTTMSQEMLRLVEAFGYGWSDLQRFTINAMKSAFISFDER
                     LSIIDDVIKPRFAVLVG"
BASE COUNT          764 a         1381 c         1466 g          712 t
ORIGIN      
        1 tcggcacgcg ctgacgcaat tgggggtgcc gtatcagtgg ggcggcaccg cgccaggcgt
       61 cggcttggac tgcagcggcc tgacgcagtg ggcgtatcac gaagcgggac tgaatatccc
      121 gcggctggcg caggagcagg acgtcggagc cgccgtcgac gccggatcgt tgcgacccgg
      181 cgaccttgcg gtgtgggacg gccacgtcgc gatgatcgtg gggaacggca cgatgataga
      241 ggccggtgac ccggtgaagc tgtccccgat ccgaacaacc aacgcggggc agggttttca
      301 gggtttctgg cgccctacgg gatgactggc gtcggcgaac gatcagccga gttcgtagga
      361 ctcgcgcgaa atagtgaagt agtgaccagt gctgctgtcg gtgcaggtca tacccgtgaa
      421 ttcgctgtcg cacctgatcg tgccgagaaa tagcgtctgg ccgtatgcga gagtctgatc
      481 cgcggcagga aactcctggc cgtagcagtt gaagccagcg ccgccgccct gcgtcagctc
      541 gaaacggtcg ccctagttca gatggcagtc cgctgaccgc ggcggcgcct cccagacgtg
      601 atcgacaatc tcgcagcgag ctgatgcttg gccgtctgaa gtgaacatgg tgcaggtgat
      661 gttgcccgat ggcgatcgga aggggccgct cgccgctcgc tgcgccgctg gtgccggtgc
      721 gggcgcgggg gcaggcgtca cggtcacggt gactgtcggt ggtggcgtca cggtcaccgt
      781 cgcggccggt gcagacggtg gagtggatgc caccgggggt gccgacgacg atgtcgatgg
      841 cgatgtcgcg ggcgtggccg acggggcgtg gaccgaaccg gccgatgaag cgcacccggc
      901 taccgtcatc gccgcaagcg cgacgagcca cacctgaata cgccatgcgc agacccgcgt
      961 cgcgcgctgc tgaacgtgca aaccttctgt catgccggta aggatcgtgg gaaggccttg
     1021 gaagattctg gtcgcgtcgc agcggctctg aatcaagcga aatgcggcgt cactagcgcg
     1081 aatagcggac agggttttcg ggcgacgaca ccgtctacgt cgccgacaac agcaaccgca
     1141 ttctgacgtt tgcaccggac ccagagtgag caccgttctt gccatccgaa ctcgatggct
     1201 attctgtgcg ccatggctgc tgacatcgtg ccgattcggc tcgggctgac caagggcgac
     1261 ctttacacgc tgtgggcgcc gcgctggcgc gatgagggcg acgaatggga ggggttcctc
     1321 ggcaaggacg aggatctgta tgccttcgag tccgtcgccg acctggtcgc cttcgtgcgg
     1381 accaacaccg acaacgacct ggccgaccac cccgcctggg aggcgttgac ccaggcgaac
     1441 gcgcacacgt tcgatccgcc agaggatcgc cagtacgacc tggtcagcct cgaagagctc
     1501 gtcgccgaga agcccagcga ggacacgatc aaggacctgc accgcgcgct ggccatcgtg
     1561 tcctcgatcg ggtcggtgtg cgagatgccc ggggtcacca agttcttcaa cggcaacccg
     1621 gtgctcggaa ccgtgggcag cggcatcgac gcgttcgccg gccgggccgg tcgcaagcgg
     1681 tgggccgaaa tcgaggcggt gatcggccgc ggctgggaca acgccatcaa cgcgatcgac
     1741 gagatcgtca ccattcccga cgtcgacaag gcggcctcga agaaggccga agccgaactg
     1801 gccgaacctg cgcccgaacc cgaggaggtc gacgaggtcg aggagatctc cgacaccgaa
     1861 gccggggcag aagacaccga cgccgaggcc gatgtcgagg aaagcgacga tctggccgac
     1921 catgccaagg ggctcgtgct cggcagcgac gacgacttct ggctcaaggt aggcatcgac
     1981 ccggtgcgga tgatgaccag cgcaggcacc tactacacct tgcgctgcta tctcgacgac
     2041 cagccaatct tcttgggccg caacggaaga atcagcgtgt tcggatccga gcgggcattg
     2101 gcgcgctatc tggccgacga gcacgaccac gacttgtccg atctggcaac gtatgacgac
     2161 atccgcacgg ccgccacgga cggctcactg gaggtagccg tcagcgacga caacgtttac
     2221 gtgctgaccg gcattgccga cgacctcgcc gacggccccg acgccatcga ccgcgaccaa
     2281 ctggagctcg ccgtcgaatt gctcagggac gtaggcgaat acgccgagga caccaccgtc
     2341 gacgaaacgc tggacgccga tcagccgctg ggccggctcg tcgcgcatgt gctcgacccg
     2401 gacaccgtcc gccgcccgag cgcgccctac gcgaaggctg tcgagcagtg ggaagcgctc
     2461 gaggcgttcg tggattcgcg gctgcgccag gagtagcgct acaactctgt cgccttcggg
     2521 tgccgcggct cgtacagacc gagatctcca ccgccgggta gccggatgct ggtcagcagg
     2581 ccccagcctt ggtcgctgac ttcgccgcat gtgacgccct tggcggcgag ttgttcgacg
     2641 gtggcagtca cgtcgtcgca catcaggtac agctcgtgag acgggccgtc cgcgggatgc
     2701 acggccacct cgccgggcgg cagcttgaag atcagccaac caccgccggc atcgacgtgc
     2761 ggatactgca gcacatcccg aaagaaagcg cggtcagcgt cggcgtcgcg gctatacaag
     2821 atcacatgtg cgccggtaat catggcgcca ccgtacgacg tggcgatgaa ttcccagcgg
     2881 gctgacggtc tgtacgggta tgaccctgcc tatcaccgtt cgttccgtca ctgttcccgg
     2941 cgcccgcctg cactacgaga tccgcggtag cggcccactg ctgctcgtca tcggctctcc
     3001 gatggcgtcg gcggagttcg cgccgctggc cggagagctg gccggcaacc acagtgttgt
     3061 cacctatgac cctcggggtt acgcggccag ccctgtcgag gatcccgacg cgcagtcctc
     3121 ggtcgagcag cgcgccgacg acgtcaacgc gatcctcgac gacctgggcg ccgactcagc
     3181 cgacgtgttc gggtccagcg gcggggcggt gaccggcctg gctttggtgg cgcggcatcc
     3241 gggacgggtg cggaccctcg tcgctcatga gccaccgctc ttggcgttgc tgcccgacgc
     3301 agcggatcag ctagcgaata ccgaggccat catcgacacg ttccaccgcg aggggttcga
     3361 ggccgcgtgg aggcacttca tgcacaacgc cggattcgac gtgtcagccg gtgatgcccc
     3421 gccgggggcg gctgagccgt cggaggatga cgtgcgccag gccgcccggt tcttcgagat
     3481 cgatatgcgg ccgaccacgc ggtacctgcc cgacgtcgac acgctgaagt ccagttcgtc
     3541 gcgcgtggtg atcggcatcg gcgtcgactc cggccggctg ctgacctacc ggacgtcgat
     3601 ggccctggcc gagctgctcg gcaccgcgcc cgtcgagttc ccgggcgacc acggcggatt
     3661 catgggcgcg ccaaaggaat tcgccgacgt attgcaccgc gtcctgtccg cctgattagc
     3721 gcgagcagac gtgaaactgc cttattcttg gcgattttgg gcagttttgc gtctgctcgc
     3781 cggagaaagc tagccgacga ggaccgcgaa gcgcggcttg atcacgtcgt cgatgatgga
     3841 cagtcgctcg tcgaacgaga tgaacgccga tttcatcgcg ttgatggtga accgctgcag
     3901 gtcgctccag ccgtagccga acgcctcgac caggcgcagc atctcctggc tcatcgtggt
     3961 gtcgctcatc aaccggttgt cggtgttgac ggtgacgcgg aaccgcagcc gagccagtcg
     4021 gtcgaacgga tgctcggcga tacttccgac ggcgccggtc tgcacgttcg acgagggaca
     4081 catctcgaac ggaactcgct tgtcacgcaa tatcgatgcc agcctgccca actgcgcggt
     4141 gccgtcggcg ccgacggtga tgtcgtcgac gatgcgcacg ccgtggccga gccggtctgc
     4201 gccgcagaac gcgatggcct cgtggatgga aggcagtccg aacgcctcgc cggcgtgaat
     4261 tgtgaagcgc gcgttgttgc ctcgcatgta ctcgaaggcg tcgagatgtc gcgtcggcgg
     4321 ata
//
LOCUS       DASRJA010000750         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2890453,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000750 DASRJA010000000
VERSION     DASRJA010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2890453"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFC01_33880"
     CDS             <1..277
                     /locus_tag="VFC01_33880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HZN84649.1"
                     /translation="VVSNIFESAGGVDGGDVAVAESQRSAMLDIKAEAMDPIAAAEAV
                     FDQAAEGRFYLLTQPDYVGSAMVERAEVLVSQRPPTLRGRRFDPAHQ"
     gene            331..1209
                     /locus_tag="VFC01_33885"
     CDS             331..1209
                     /locus_tag="VFC01_33885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN84650.1"
                     /translation="MRQRGLAAVRLAIESAPLPDGMPDMAVVEDCTASGPAGPIPLRI
                     YRPTTAANAPVLLYFHGGGMVLGSNHSFEPLARMLASVSDATVVAVDYRLAPENPPPA
                     QFDDAYSATEWVAANADRLGVDPARLAVVGDSAGGSLAAAVALAARDHGGPAILCQVL
                     LYPGLDRDMAAPSIVALRDAPLLDHDDIVYMHDLADRGACVPHDHYRVPAYATDLTGL
                     PPAIIVTAQCDPIRDWGERYAARLRDAGVQTTVTRYPGMYHGFLMRSDATARGRLAIA
                     EVGGLLRAKFAHPLPF"
     gene            1291..>2208
                     /locus_tag="VFC01_33890"
     CDS             1291..>2208
                     /locus_tag="VFC01_33890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84651.1"
                     /translation="MLTDERRMELSEVLRPASPPVEIDDVYTDDQRARLLDVVRSQGP
                     WKLIIAQHFASADELMATMSGAFPEGFTPSLDLFLTPTFRGYLANYATVLYPELHDCF
                     YNAEFLQMAKQYWKAEYAKPEMMLFNINGPCANRDPGHLDSPSFRGVRHENAPTWLCS
                     VMGKSGLFQDYLIKMAQVITWFSLDEGSGFTYWPDGPLKAPQRLLPPVYNRGVVVQNE
                     MMVHRGEASGPLEQQTPAGLAFDTVFTGDPDHRDTWLLKNGDDVIVRHHTNELRFLVH
                     WSAEVFSDYDELKKNMDGSDDLTIERAIDT"
BASE COUNT          387 a          749 c          702 g          370 t
ORIGIN      
        1 ggtggtgtcg aacatctttg agtccgccgg tggcgtagac ggcggcgacg tcgcggtcgc
       61 cgaatcgcaa cgctccgcca tgctcgacat caaggccgag gcaatggacc ccatagcggc
      121 ggccgaggcc gtgttcgacc aggccgccga aggacggttc tacctgttga cccagccaga
      181 ctacgtcggc tcggcgatgg tcgagcgtgc cgaagtcctt gtctcccaac ggccgccgac
      241 gttgaggggc cgtcgcttcg accccgcaca ccagtgagac tcgaccctga cgccgccgca
      301 cgcgtcgcgt ccttcggtga tatcccgccg atgcgccaac ggggcctggc cgcggtgcgc
      361 ctggccatcg aatcggctcc cctacccgac ggcatgccgg acatggcggt cgtcgaggac
      421 tgcacggcgt cgggcccggc aggcccgatc ccgttgcgga tctaccgccc gaccactgcg
      481 gcgaacgcac cggtgctgct gtacttccac ggcggcggaa tggttctcgg ctccaaccac
      541 tccttcgaac cactagcccg aatgctggcc tccgtcagcg acgcgaccgt cgtggccgtc
      601 gattaccgac tggcgccgga gaatccaccg cccgcgcagt tcgacgacgc ctattcggcc
      661 accgagtggg tggcggcaaa cgctgaccga ctgggcgttg acccggcccg gcttgcggtg
      721 gtcggcgaca gcgcgggcgg ctcgctggcc gccgccgtcg cgttggccgc gcgcgatcat
      781 ggcggaccgg cgatcttgtg ccaggtcctg ctctatcccg gtctggaccg cgacatggcg
      841 gcaccgtcga tcgtcgcgct cagggacgcg ccgttgctcg accacgacga catcgtctac
      901 atgcacgacc tggcggaccg cggcgcatgc gtaccgcatg accactaccg ggttcctgcc
      961 tacgcgacgg acctgaccgg actgccgccc gcgatcatcg ttaccgcgca atgcgacccg
     1021 atccgcgact ggggcgagcg ctacgccgcc aggctgcgag acgcaggcgt acaaacgacc
     1081 gtcacccgtt acccaggcat gtaccacgga ttcctgatgc gctccgatgc cacggcgcgg
     1141 ggccggctgg cgatcgccga ggttggcggg ctcttgcgtg cgaaattcgc ccatcccctg
     1201 ccgttttagc ctcccgatca ccggacaggt ccgcactgca ccgcccattt cgatcacaga
     1261 atcgacgtag ccgagaaaga ggagaccctg atgctcactg atgagcggcg catggagcta
     1321 tccgaggtgt tgcggcccgc gtcaccaccg gtcgagatcg atgacgtcta caccgacgac
     1381 caacgggctc gcctgcttga cgtggtccgc agtcaagggc cgtggaagct gatcatcgct
     1441 cagcatttcg cgtcggccga cgagctgatg gcgacgatga gtggcgcgtt ccctgaagga
     1501 ttcacgccgt cgttggacct gtttctaact ccaacctttc ggggctatct cgccaactac
     1561 gcgacagtgc tgtacccgga actgcacgac tgcttctaca acgccgagtt tctgcagatg
     1621 gcgaagcagt actggaaagc cgagtacgcc aagccggaga tgatgctgtt caacatcaac
     1681 ggaccgtgcg ccaaccgcga tccggggcac ttggattcgc cgagcttccg cggagtgcgt
     1741 cacgagaatg cgccgacttg gttgtgcagt gtgatgggca aatccggtct gttccaggat
     1801 tacctgatca agatggcgca ggtgatcacg tggttctcgc tggatgaggg cagcggtttc
     1861 acttactggc cggacggccc gctcaaggcg ccgcagcgcc tgctgccgcc ggtgtacaac
     1921 cgcggcgtcg tcgtgcagaa cgagatgatg gtgcaccgcg gcgaggccag cggcccgctc
     1981 gagcagcaga cgcccgccgg gctcgcgttc gacacggtgt tcaccggcga ccccgaccac
     2041 cgggacacgt ggctactcaa gaacggcgat gacgtcatcg tcagacacca caccaacgaa
     2101 ctgcgcttcc tcgtgcactg gtcggcggaa gtgttctccg actacgacga gctaaagaag
     2161 aacatggacg gctccgacga cctgaccatc gagcgggcga tcgatacg
//
LOCUS       DASRJA010000751         6337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2079863,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000751 DASRJA010000000
VERSION     DASRJA010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079863"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VFC01_33895"
     CDS             complement(<1..1118)
                     /locus_tag="VFC01_33895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZN84652.1"
                     /translation="MRLRRPMRGVIVLLCAALLVGSGCARFDEAQSQPFTTEPELKPG
                     PPSTPPPPPPLPPKPFPKECPAPGVMQGCLESTSGLIMLPDSQSALVAERTTGAVKEV
                     SVSAEPKVKLVIPVDPSGDGGLMDIVMSPTYSQDRLMYAYVSTPTDNRVIRIADGDIP
                     KPILTGIPKGATGNTGALIFTSPTTLDVLTGDAGNPAQAADPGSLAGKLLRIEQPTTV
                     NQAPTTTALSGIGAGGGLCIDHSDGSLYVTDRTPTADRLQRITKDSKISTVWTWPDRP
                     GVAGCAALDGTVLVNLVNTKQTVAVRLAPDTGAVTGEPEVVRQDQHGHAFALQLSPDG
                     NVWGATVNKTSGDAEKLDDVVFPLFPQGGGFPRSNADNT"
     gene            1259..2062
                     /locus_tag="VFC01_33900"
     CDS             1259..2062
                     /locus_tag="VFC01_33900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZN84653.1"
                     /translation="MTSHPDPSNWQRPASASLVDPEDDLPSSNYAGDFETTAIPRYDS
                     AKSSSGEPFGLISDPEPLPYVQPGGRHSLGSYGAEPIEIESDAADRAHDRRGTQDLGL
                     LLLRLAVGALLIGHGLQKAFGLWGGPGFDGFEQSLTDQGFQHADILTYVATGGQIAAG
                     VFLVLGLLTPVAAAGALAYSVTGILAEAMMAHEEARLASFLTDGHEYKLVFVCAVAAI
                     ILIGPGNYGFDAGRGWVRRPFIGSFTALVLGIGAGVAAWVLLNGGNPLA"
     gene            complement(2059..2409)
                     /locus_tag="VFC01_33905"
     CDS             complement(2059..2409)
                     /locus_tag="VFC01_33905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZN84654.1"
                     /translation="MAHFAVGFLALGLLALVLAGPSGFALLLVIPVVLSTLVVRYRTV
                     ADRDTVTARTLLGSQTVSWNDIAGLRFGKGSWAYAQLTDGGELRLPAVTFATLPLLTE
                     ASGGRVPNPYAQEG"
     gene            2706..4583
                     /locus_tag="VFC01_33910"
     CDS             2706..4583
                     /locus_tag="VFC01_33910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HZN84655.1"
                     /translation="MSAPTTRPQEQPAPAPNGKHAAPAAAKSASKASPAQPKRVAPHQ
                     LTGAQAVIRSLEELDVDVIFGIPGGAVLPVYDPLFDSQKLRHVLVRHEQGAGHAASGY
                     AHATGKVGVCMATSGPGATNLVTPLADAHMDSIPMVAITGQVGRALIGTDAFQEADIS
                     GITMPITKHNFLVRSGDDIPQAIAEAFHIASSGRPGPVLVDIPKDVLQGQCTFSWPPQ
                     IDLPGYKPNTKPHNRQVREAAKLIAAARKPVLYVGGGVIRGEATEQLLDLAELTGIPV
                     VTTLMARGAFPDSHPQHMGMPGMHGTVAAVAALQRSDLLIALGTRFDDRVTGKLDSFA
                     PEAKVIHADIDPAEIGKNRHADVPIVGDIKAVITDLIEALRKDGTIGSIKIDNWWEYL
                     RGVQSTYPLSYGPQSDGSLSPEYVIETLGKMAGPDAIYVAGVGQHQMWAAQFISYENP
                     KTWLNSGGLGTMGFAVPAAMGAKFGRPDAEVWAIDGDGCFQMTNQELATCAVEGAPIK
                     VAIINNGNLGMVRQWQTLFYEERYSQTDLATHSHRIPDFVMLAEALGCVGLRCERAED
                     VEDVIKKAREINDRPVVIDFIVGADAQVWPMVAAGTSNDEIQAARGIRPLFDDPEEGH
                     A"
     gene            4583..5089
                     /gene="ilvN"
                     /locus_tag="VFC01_33915"
     CDS             4583..5089
                     /gene="ilvN"
                     /locus_tag="VFC01_33915"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZN84656.1"
                     /translation="MTVNTHTLSVLVEDKPGVLARVASLFSRRGFNIQSLAVGATEQK
                     DMSRMTIVVSVEDSPLEQITKQLNKLVNVIKIVEQDVDNSVDRELALIKVRADATTRG
                     QVIEAVNLFRAKVVDVSTESLTIEATGTPAKLEAFLRVLEPYGIRELVQSGVVSLSRG
                     PRGIGTVK"
     gene            5134..6135
                     /gene="ilvC"
                     /locus_tag="VFC01_33920"
     CDS             5134..6135
                     /gene="ilvC"
                     /locus_tag="VFC01_33920"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZN84657.1"
                     /translation="MFYDDDADLSIIQGRKVGVIGYGSQGHAHSLSLRDSGVQVKVGL
                     KEGSKSRDKVTEQGLEVDTPAEVAKWADVIMLLAPDTAQADIFRNDIEPNLEDGNALF
                     FGHGLNIHFDLIKPPANVTIGMVAPKGPGHLVRRQFVDGKGVPCLIAVDQDPKGEGQA
                     LALSYAKGIGGTRAGVIKTTFKDETETDLFGEQAVLCGGTEELVKTGFDVMVEAGYAP
                     ELAYFEVLHELKLIVDLMYEGGIARMNYSVSDTAEFGGYLSGPRVIDAGTKERMKQLL
                     TEIQDGTFVKKLVANVEGGNKELEGLRKRNAEHPIEVTGKKLRDLMSWVDRPLTDTA"
     gene            complement(6199..>6337)
                     /locus_tag="VFC01_33925"
     CDS             complement(6199..>6337)
                     /locus_tag="VFC01_33925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H dehydrogenase"
                     /protein_id="HZN84658.1"
                     /translation="FVDGNPYGVSLIADHDNISEFDDATNNALDHLAKRVVAVADRLS
                     S"
BASE COUNT         1102 a         2039 c         2127 g         1069 t
ORIGIN      
        1 gtgttgtcgg cgttgctgcg cgggaatccg ccgccctgcg ggaacagcgg gaacacgacg
       61 tcgtcgagct tctcggcgtc gccggacgtc ttgttgaccg tcgcgcccca cacgttgccg
      121 tcgggtgaga gctgtagcgc aaatgcatgc ccgtgctggt cctgacggac cacctctggc
      181 tcacccgtga ccgcgccggt gtccggcgcc agccgcacgg ccaccgtctg tttggtattg
      241 acgaggttca ccagcaccgt gccgtcgagt gcggcgcagc cggccacgcc agggcggtcg
      301 ggccacgtcc acaccgtcga gatcttcgag tccttggtga tgcgctgcag ccggtcggcg
      361 gtgggggtgc ggtcggtgac gtacagagag ccgtcggagt ggtcgatgca caggccgcca
      421 cccgcgccga tgcccgacag cgcggttgtc gtcggcgcct ggttgaccgt ggtgggctgc
      481 tcgatgcgca acagctttcc ggccagcgaa ccgggatcgg cggcctgtgc gggattcccc
      541 gcgtcgccgg tgagcacgtc gagcgtcgtc gggctggtga agatcagcgc cccggtgttc
      601 ccggtcgcgc ccttcgggat gccggtcagg atcggcttcg ggatatcgcc gtcggcgatg
      661 cggatgacgc ggttgtcggt cggggtgctg acgtaggcgt acatcagccg gtcctgggag
      721 taggtcggcg acatcacgat gtccatcagg ccgccgtcgc ctgagggatc gaccgggatc
      781 accagcttga ccttgggctc ggcgctgacc gacacctcct tcaccgcgcc ggtcgtgcgc
      841 tcggcgacca gcgccgactg gctgtcagga agcatgatca gcccgctggt gctctccagg
      901 cagccctgca tgacgccggg cgctgggcat tccttcggga atggcttggg gggcagcggc
      961 ggcggaggcg gcggtgtcga tgggggaccg ggcttgagtt ccggttcggt ggtgaatggc
     1021 tgcgactgtg cctcgtcgaa gcgagcgcat ccggacccga cgagcaacgc cgcacacagc
     1081 aggacaatca caccccgcat gggccggcgc aatctcatgc aggccaggtt acggattgat
     1141 ccgtagtgcg cgccacgctc gccccgtgac gcggccgaga cgaccctcgc gaacagggtc
     1201 gtcaaaagcg ccgcgccaat cctcgattcg cgggtgacta gcacttaccc tggcaagcgt
     1261 gaccagtcat cctgatccaa gtaactggca gcgccccgcg tctgcgagcc tggtcgatcc
     1321 cgaggatgat cttccgtcct cgaactacgc cggtgatttc gagaccaccg cgatcccccg
     1381 ctacgactcg gccaagtcgt cgagtggtga accgttcggc ctgatcagcg acccggagcc
     1441 gctgccgtac gtccagcccg gcggtcggca ctcactcggc tcctatggag cggagcccat
     1501 cgagatcgag tcggatgccg ccgaccgggc tcacgaccgg cgcggcacgc aggaccttgg
     1561 ccttcttctc ttgcgtctgg ccgtcggggc gttgctcatc gggcacggcc tgcagaaggc
     1621 gtttggcttg tggggcggtc ccggcttcga cggctttgag cagtcgctga ccgaccaggg
     1681 cttccagcac gccgacatcc tgacgtacgt ggcgacgggc ggccagattg ccgccggggt
     1741 atttctggtg ctgggcctgc tgactccggt cgcagccgcc ggcgcgctgg cgtactcggt
     1801 caccggcatc ttggcggagg ccatgatggc ccacgaagag gccaggttgg cgtcattcct
     1861 gaccgacggc cacgagtaca agctcgtctt tgtctgtgct gtcgcggcga tcatcctgat
     1921 cggcccgggt aactacggat tcgacgccgg tcgcggctgg gtgcgccgtc cgttcatcgg
     1981 atcgttcacc gcgcttgtgc tcggcatcgg cgcaggcgtc gcggcgtggg tgctgctcaa
     2041 cggcggcaac ccgctggcct agccctcttg cgcgtacggg ttcggaaccc tgccgccgct
     2101 ggcctcggtc agtaacggca gcgtcgcaaa cgtcaccgcc ggcagcctca gttcgccccc
     2161 gtcggtgagt tgggcgtatg cccatgaccc cttgccgaag cgcagccccg cgatgtcgtt
     2221 ccacgagact gtctgacttc ccagaagtgt gcgcgcggtg accgtgtcgc ggtcggccac
     2281 cgtccggtag cgaacgacca acgtcgacag gacgaccgga atgacgagca gcagcgcgaa
     2341 ccccgacggc ccggcgagca ccagtgccag cagaccaagc gccaagaagc caacggcgaa
     2401 gtgagccatc ggcgagatcc tgatgacgac ggcgttctgc tcagagtcgg cggccacgtc
     2461 gtcatcttcg cacctggccg aaatttgacc tgtgaccagt gcggaggcta ccgtcgacgg
     2521 ttatgcagac cctggcagtg ctcgtagtaa ttgggaagcg cgttgatgcc gcgcttgcct
     2581 tctgccgggc atagcaacca gcatcaacgc gcgaccctcg tacagcggcc cagctgacgg
     2641 gggttttttc ttgccccaac accgatttga gatcctagag agacgacgga gataaagagg
     2701 acatcgtgag cgcacctacc acgcgaccgc aggagcagcc ggcgcctgca cccaacggga
     2761 aacacgccgc gcctgccgcc gccaaatcgg cgtcgaaggc tagccccgcg cagccgaaac
     2821 gcgttgcgcc gcaccaactc accggtgcac aggccgtcat ccgctcgctg gaggaactcg
     2881 acgtcgacgt catcttcggc attcccggtg gtgccgtgtt gccggtctac gacccgcttt
     2941 tcgactcgca gaagctgcgg cacgtgttgg ttcgccacga gcagggtgcc ggccatgccg
     3001 ccagcggtta cgcgcatgcc accggcaagg tcggggtgtg catggcgacg tcgggtcccg
     3061 gcgccaccaa tctggtcact ccactggccg atgcgcacat ggactcgatc ccgatggtgg
     3121 ccatcacggg tcaggtcggc cgtgcgctga tcggcaccga cgccttccag gaagccgata
     3181 tctccggcat caccatgccg atcaccaagc acaacttcct ggtccgcagt ggcgacgaca
     3241 tcccgcaggc gatcgccgag gcgttccaca tcgcctcgtc cggtcggccg ggtccggtgc
     3301 tggtcgacat cccgaaggac gtgctgcaag gacagtgcac cttcagctgg ccgccgcaga
     3361 tcgacctgcc cggctacaag ccgaacacca agccgcataa ccgccaggtt cgcgaggcgg
     3421 ccaagctgat cgcggcggcc cgcaagccgg tgctctacgt cggcggcggc gtgatccgcg
     3481 gcgaggccac cgaacagctg ctggacctgg ccgagctgac cggcatcccc gtcgtcacca
     3541 cgctgatggc gcgcggggcg ttcccggaca gccatccgca gcacatgggc atgccgggca
     3601 tgcacggcac ggttgcggcg gtggccgcgt tgcagcgcag tgatctgctg atcgcgctgg
     3661 gcacccgctt cgatgaccgg gtgacgggca agctggattc ctttgcgccc gaggccaaag
     3721 tgatccacgc cgacatcgac cccgccgaga tcggtaagaa ccggcatgcc gacgtgccga
     3781 tcgtcggcga tatcaaggct gtcatcaccg acctgatcga ggcgctgcgc aaagacggca
     3841 ccatcggctc gatcaagatc gacaactggt gggagtacct gcgcggtgtg cagtcgacgt
     3901 acccgctcag ctacggcccg cagagcgacg gcagcctgtc gccggagtac gtgatcgaga
     3961 cgctcggcaa gatggccggc ccggacgcca tctacgtcgc aggcgtgggg cagcaccaga
     4021 tgtgggccgc gcagttcatc tcctacgaga acccgaagac gtggctgaac tccggcggtc
     4081 tcgggaccat gggcttcgcg gtgcctgcgg cgatgggcgc caagtttggc cggcccgatg
     4141 ccgaggtgtg ggccatcgac ggcgacggct gcttccagat gaccaatcag gaactggcca
     4201 cctgcgccgt cgagggcgcg ccgatcaagg tggccatcat caacaacggc aacctgggca
     4261 tggtgcgtca gtggcagacg ctgttctacg aagagcggta cagccaaacg gatctggcca
     4321 cgcattcgca ccgcatcccg gatttcgtga tgctggccga ggcgctcggc tgcgtcggat
     4381 tgcggtgtga gcgcgccgaa gacgtcgagg atgtcatcaa gaaggcgcgt gagatcaacg
     4441 accggccggt ggtgatcgat ttcatcgtcg gcgccgacgc ccaggtgtgg ccgatggtgg
     4501 ccgcgggcac cagcaatgac gagatccagg ctgcgcgcgg catccgtccg ctgttcgacg
     4561 atcctgaaga agggcacgcg tgatgaccgt caatacgcat acgttgtcgg tgcttgtcga
     4621 ggacaaaccc ggcgtgctcg cccgagtggc gtcgctgttc tcgcggcgcg gcttcaatat
     4681 tcagtcgctg gcggtcggtg cgaccgagca gaaggacatg tcgcggatga cgatcgtcgt
     4741 cagcgtcgag gattcgccgc tcgagcagat caccaagcag ctgaacaagc tcgtcaacgt
     4801 gatcaagatc gtcgagcagg acgtcgacaa ctcggtcgac cgtgaactcg ccctgatcaa
     4861 ggtgcgtgcc gatgcgacta cccgtggtca ggtcatcgag gcggtgaacc tgttccgcgc
     4921 caaggtcgtt gacgtgtcga cagagtcgct gaccatcgag gcgaccggta ctcccgcgaa
     4981 gctggaagcc ttcctgcgag tgctggagcc gtatggaatc cgtgagctcg tgcagtccgg
     5041 cgtggtctcg ctgtcacgcg gcccgcgtgg catcggcacc gtcaagtaag acccaaacca
     5101 agtagaagat aaggaaataa cgtggcagtt gagatgttct atgacgacga cgcggacctg
     5161 tcgatcattc agggacgtaa ggtcggcgtc atcggctacg gcagccaggg ccatgcgcac
     5221 tcgctgtccc tgcgcgactc cggtgtgcag gtaaaggtag gcctcaagga aggctcgaag
     5281 tcccgggaca aggtcaccga gcagggcctc gaggtggata cgcccgccga ggtggccaag
     5341 tgggccgacg tgatcatgct gctggcgccc gataccgcgc aggccgacat cttccgcaac
     5401 gacatcgagc cgaatctgga ggacggcaac gcgttgttct tcggccacgg tctcaacatc
     5461 cacttcgatc tgatcaagcc gccggccaac gtcaccatcg gcatggtcgc cccgaagggc
     5521 cccggccacc tggttcgtcg ccagttcgtc gatggcaagg gcgtgccctg cctgatcgcg
     5581 gtcgaccagg atcccaaggg tgaagggcag gcgctcgcgc tgtcctacgc caagggcatc
     5641 ggcggcacca gggccggcgt catcaagacc acgttcaagg atgagaccga gaccgatctg
     5701 ttcggtgagc aggccgtgtt gtgcggcggc acagaggaac tcgtcaagac gggcttcgac
     5761 gtgatggtcg aggcgggcta cgcgcccgag ctggcgtact tcgaggtgct gcatgagctg
     5821 aagctgatcg tcgacctgat gtacgagggc ggcatcgcgc ggatgaacta ctcggtgtcc
     5881 gacaccgcgg agttcggcgg ctacctgtcc ggcccgcgcg tgatcgacgc cggcaccaag
     5941 gagcggatga agcagctgct gaccgagatt caggacggca cattcgtcaa gaagctcgtt
     6001 gccaatgtcg aaggcggcaa caaggagctc gagggactgc ggaagcgcaa cgccgagcac
     6061 ccgatcgagg tgaccggcaa gaagctgcgc gacctgatga gttgggtcga ccgcccgctc
     6121 accgacaccg cttagcggtg tccgtagtga tttcggcgcg ctaaatcacg ctgagcgacg
     6181 gttagcgcgc ccaaatcgct acgaactgag gcggtccgcg accgcgacga cgcgcttggc
     6241 cagatggtcg agcgcgttgt tggttgcgtc gtcgaactcg ctgatgttgt cgtgatcggc
     6301 gatgaggctc accccgtatg ggtttccgtc gacgaac
//
LOCUS       DASRJA010000752        20452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2955337,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000752 DASRJA010000000
VERSION     DASRJA010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 20452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20452
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2955337"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFC01_33930"
     CDS             <1..243
                     /locus_tag="VFC01_33930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84659.1"
                     /translation="PSSPMAGAGPQTATPSPSPATRSCTHPLKGHHDISATAATTAST
                     RSPLSSAPPHHSPIEDVTKRLKLADTASMPTPPQRH"
     gene            322..618
                     /locus_tag="VFC01_33935"
     CDS             322..618
                     /locus_tag="VFC01_33935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84660.1"
                     /translation="MSFSQTTVGWLLLGAAALTFAVGFASRTIIGRRRNTKGVRRLVH
                     MFRRTGVARVLFGRFEGDVLDDDELDSMILMPTIVVGCGLCLTAIFLLGYEVLA"
     gene            complement(623..3796)
                     /locus_tag="VFC01_33940"
     CDS             complement(623..3796)
                     /locus_tag="VFC01_33940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN84661.1"
                     /translation="MTAAAIVCTSCGAEPARAGARFCDACGGALPAPARAEFKQVTVL
                     FADVVGSMDIAAAVGAERLREIMAGVVGRGAAVVQRYGGTMDKFTGDGLMAVFGAPKS
                     LEDHAIRACLAALDLQSETLRLADEVQRRDGIDLELRVGLNSGQVVAGEIGSGVPGYT
                     AIGEQVGFAQRMESAAPPGGVMLSESTAHLVDKTAVLADPELVRIKGRDEPVPARRLF
                     AVVSGHGAAAGTESTLVGRRWEMAALEAMVEQAIRGRGDVVGMAGPPGIGKSRVAREA
                     AALAMNRGFEVVWTYCESHAKDVPFLVVSNLLRTVIGVTDLDGTDARARIRERIPDAD
                     PQDLLLLDDLLGAADPDVALPPIDPDARRRRLTALINAASLARTTPALYIIEDAHWID
                     AVSESMLTDFLTALPRTLSMALITCRPEYEGALTRVPGAQTIALAPLDDSETVTLLAE
                     LLGPDPSVGELGEIMVSRVGGIPFFAEEIVRDLTERGVLRGQRGAYVCRTPVTEVSVP
                     ATLQATIGARIDRLDAPAKRVLSAAAVIGSRFDTELLTGLGVEPVVDELIGAELIDQV
                     RFTPRAQYAFHHPLIRAVAYESQLKSDRAEWHRRLAVAIQQRDPDSVDESAALIAEHL
                     QAAGDLRAAYDWHMRASAWSTNRDITAARVSWERARQIADALPDDDPGRTAMRIAPRT
                     MLSVSSWRAAESDSSERFEELRDLCTGAGDKASLAIGMTGPVSELMWSGHTRDASRLA
                     SEQMALLESIADPALTIGAAYGVMVIKAAACEIADLLHWSQTVIDLADGDPTKGANFA
                     MGSPLAAAIALRGFGRSWLGRPGWREDFDDASAMARRSDPVTHALLITGINRLAIENG
                     VLRADEFPLREIEETLRIAERSSGDTALSTVKLSFGAALTYRSTLADRRRGLELVTQV
                     RDTWLRERTRLYLVPTAELAIARERAGRGERDRAIAMMRSALEKLFQMQQLGLCVVPT
                     ADLVEALLERRADGDIAEAQRSIDRLVNMPDYAGSAVCEIWLLRLRALLCRARGEDIA
                     YQDFVNRYRTMADSLGFERHIAMAEAM"
     gene            complement(3870..4610)
                     /locus_tag="VFC01_33945"
     CDS             complement(3870..4610)
                     /locus_tag="VFC01_33945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN84662.1"
                     /translation="MAPALTAITDSVHFAYTDLVNWTLVTDGNGVMLIDAGFPGHRDD
                     VVDSLRQLGLGVDDLRAILLTHAHIDHLGTAIWFAKTHGTPVYCHAAEVGHSKREYLE
                     QAGPADIIPHLWQPRYLKWTAAISRKGGMVRDGIPSTQALTEDVAAGLPGTPMAIPTP
                     GHTGGHCSFVVDGVLVSGDALVTGHPLLTRDGPQLLPDLFNHDQDGCVRSLAALGLLD
                     TDVLLPGHGPVWRGSIREAAEQAASRQL"
     gene            4647..5657
                     /gene="fgd"
                     /locus_tag="VFC01_33950"
     CDS             4647..5657
                     /gene="fgd"
                     /locus_tag="VFC01_33950"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HZN84663.1"
                     /translation="MAELKLGYKASAEQFAPRELVELAVAAEEHGMDSATVSDHFQPW
                     RHEGGHAPWSLAWMAAVGERTKRLILGTSVLTPTFRYNPAVIAQAFATMGCLYPDRIF
                     LGVGTGEALNEIATGYEGEWPEFKERYARLRESVKLMRELWLGDRVDFDGEYYRTKGA
                     SIYDVPEGGIPIYIAAGGPQVAKYAGRAGDGFICTSGKGEELYKDKLIPAMKEGAEAA
                     GRNPDDIDRMIEIKISYDPDPTLALENTRFWAPLSLTAEQKHSIDDPIEMEKAADALP
                     IEQVAKRWIVASDPDEAVEKVGQYVTWGLNHLVFHAPGHDQRRFLELFQKDLEPRLRR
                     LG"
     gene            5676..7739
                     /gene="pta"
                     /locus_tag="VFC01_33955"
     CDS             5676..7739
                     /gene="pta"
                     /locus_tag="VFC01_33955"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HZN84664.1"
                     /translation="MSTTAIYIASPEGDTGKSTIALGILHRLAATVAKVGVFRPITRL
                     GEDRDYILELLLAHTTAGLPYEECIGVGYQQLHEDPDGAIADIVDRYHHVAGQCDAVL
                     IVGSDYTDVATPSELSMNARIAVNLGAPVVLAVKAKGRTPEQVAQVVDLCLFELDAQH
                     AHTAAVVANRCDPAHMTAVAEALKPLGPKTYVLPEEPLLVAPSVAELGAAVEGTLLRG
                     DPALLTREAMDVLVAGMTAEHVVERLTEGVAVITPGDRSDVVLAVVSAHAAEGFPSLS
                     CVILNGGLELHPSIASLVAGLGLRLPMIATRFGTFETASRVASARGRVTASSQRKIDT
                     AIALMDTHVDTADLLAQLAISIPSVVTPQMFTYQLLDRARSVRKRIVLPEGEDDRILK
                     AAGRLLQRSVADLTILGDEPQIRARAAELGVDLSTAKVLNPRTSDLCDKFAEQYAELR
                     KRKGVTVEQAREIIHDVSYFGTMLVHNNMVDGMVSGATHTTAHTVRPAFEIIKTLPDV
                     STVSSIFLMCLADRVLAYGDCAIVPDPTSEQLADIAISSARTAAQFGIDPRVAMLSYS
                     TGTSGTGADVDKVRAATELVRKREPDLLVEGPIQYDAAVDPTVAKAKMPDSKVAGRAT
                     VLIFPDLNTGNNTYKAVQRSAGAIAIGPVLQGLNKPVNDLSRGALVEDIVNTVAITAI
                     QAQSL"
     gene            7736..8923
                     /locus_tag="VFC01_33960"
     CDS             7736..8923
                     /locus_tag="VFC01_33960"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HZN84665.1"
                     /translation="MSRSVLVLNSGSSSVKFQLIDPDSGASLADGIVERIGEDESAAS
                     LTVGENEVTRDDRVADHEAALRTAFEMFDEAGTHVDTVGLVAVGHRVVHGGPDLYRPT
                     LVDDALIAKLEDLAPLAPLHNPPAVLGIRVARKALPDLPHVAVFDTAFFHDLPPAAAT
                     YAIDRDTAERWHIRRYGFHGTSHQYVSEQAAAFLDVPLVKLNQIVLHLGNGASASAIV
                     GGRPVDTSMGLTPLEGLVMGTRSGDIDPGVVTYLWRTAKMSVEDIESMLNRRSGVRGL
                     GGEIDFRVLHQRIESGDETAQLAYDVYIHRLRKYIGAYLALLGTADVITFTAGVGEND
                     AAVRRDALSGMAALGIELDEHLNASPARAARRISADKSPTTVLVVPTNEELTIARACV
                     EVI"
     gene            8959..9603
                     /locus_tag="VFC01_33965"
     CDS             8959..9603
                     /locus_tag="VFC01_33965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84666.1"
                     /translation="MATPDRESVLNELEVLATLEHALIVEFLSIACALGLDLDAQDGG
                     PTTDRGRDAASAASVRAFTEMFHFKCVNQALVGSGRPPRMDRAEEVTTTSGQVSPIPP
                     DQAGFERLLEHERSLAAEVDDRYAEVAAVLTTVFDGDELNEWRDVLVDNANHVGDLAD
                     LQTALDGVAPADFLRATRRTPNDGFEERMLDESPDLRSDRACAPGQFRSGFRCR"
     gene            complement(9724..10167)
                     /locus_tag="VFC01_33970"
     CDS             complement(9724..10167)
                     /locus_tag="VFC01_33970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN84667.1"
                     /translation="MNSAVGTCRLFRAEPQSLDRREEHHRAIFSACHVEDTTVLVTVR
                     GEVDATNNRALAAYVERQVVGSTRLVLDLSVIEFFGAAGFAALHNVNVICARYGVSWV
                     LVAGPQLRRFLRICDPENLLPLQDSAVNRLETASRDRQLLVGGNH"
     gene            complement(10375..12630)
                     /locus_tag="VFC01_33975"
     CDS             complement(10375..12630)
                     /locus_tag="VFC01_33975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase PknG"
                     /protein_id="HZN84668.1"
                     /translation="MAEAKDYDESPGTQPASFDDLGMDSMSTMRPMATQAIFRPRLDD
                     DVDSTSVGTEDTEPHDHLTTVTRALSPTRRLGGGLVEIPRVPAKDPLEALMTNPVVAE
                     SKRFCWNCGRPVGRSSPDGRALSEGWCPHCGSAYSFLPQLNPDDEVAEQYVIKGCIAH
                     GGLGWVYLAYDKNVNDRPVVLKGLVHSGDAEAQTIAMAERQFLAEVTHPGIVKIYNFV
                     EHDDKHGNPVGYIVMEYVGGTSLKQAKGDRLPVAEAIGFMMEILPAMGYLHSIGLIYN
                     DLKPENIMVTEEQLKLIDLGAVSRVNSFGYLYGTPGYQAPEIVRTGPTVQTDIYTVGR
                     TLAALTLNLRTRKGRYVDGLPEDDPVLREYDSYSRLLRRAIDPDPRRRFGSAEEMSAQ
                     LLGVLREVVAQDTGVPRPGLSTVFSPSRSTFGVDLLVAHTDVYLDGQVHSEKLTAQEI
                     IKALPVPLVDPADVGAAVLSASVLSQPVQTLDQLRAARHGALDSEGIDLSESVELPLM
                     EVRALLDLGDVAKATRKLDDLAGRVGWRWRLVWFRAVAEMLSADFESASKHFTEVLDT
                     LPGELAPKLALAATAELAGSADERKFYKTVWCTDNGVISAGFGLARAQSAEGDRDSAV
                     QTLDEVPATSRHFTTARLTSAVTLLSGRSMSEITEQQIRDAARRVEALPDTEPRVLQI
                     RALVLGTAMDWLADNTASTNHILGFPFTEHGLQLGVEASLRSLARVAPTQAHRYALID
                     LANSVRPTSTF"
     gene            complement(12630..13619)
                     /locus_tag="VFC01_33980"
     CDS             complement(12630..13619)
                     /locus_tag="VFC01_33980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZN84669.1"
                     /translation="MSARAKNRRTGGAIPVFLLAAAAVLTGCAQGAAVMPSPGVTLAP
                     PTPAGMQELPPAPPKAPEDEGDCNRTASLRPFSNKAEADAAVATIRNRGRLIVGLDIG
                     SNLFSFRDPITGEITGFDVDIAGEIARDIFGSPSQVEYRILSSADRIAALQNNQVDVV
                     VKTMTITCERKKQVGFSTVYLNANQRILAARDSSISQPSDLSGKRVCVAKGTTSLDRI
                     QQISPPPMIVGVVTWADCLVALQQRQVDAVSTDDSILAGLVSQDPYLHIVGPSLNEEP
                     YGIGVNLKNTGLVRFVNGTLERIRRDGTWYTLYRKWLTVLGPAPAPPVARYSD"
     gene            complement(13619..14938)
                     /gene="glnX"
                     /locus_tag="VFC01_33985"
     CDS             complement(13619..14938)
                     /gene="glnX"
                     /locus_tag="VFC01_33985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase G-activating protein GlnX"
                     /protein_id="HZN84670.1"
                     /translation="MTVELAHPSTEPLASRSPATPAHPRWWFLWTTPGRILTIGVVLS
                     ALVIASAFATSTTINDRQEALTTVLNHTEPLSFAAGQLYTTLSVADAAAATAFIAGSE
                     PRDVRQRYEQAITDASVAVTRASSGLTDEPMVQLLGRVNAQLSVYTGLVETARTNNRA
                     GNPVGSSYLSEASSLMQTQMLPDAQRLYEETSARVDAETTASARIPGPVILVVLATLL
                     FGAFANRWLARRTRRRVNIGFVAGGLAVLIMLIWVGTALVISTADSRSAKDTAAESLK
                     TVTSLAITAQQARADETLSLIRRGDEDVRKQSYYQRIDSMQQQLSDYLSRSDAIDKAD
                     LADAEQLLKRWRAADDRINAYIAVGNYQAATQVALGTGEDDSTPAFDKLDTALTKGIG
                     ESRSQLRNDILNARRVLSGATVGAAVLSVVAALAVALGLWPRLSEYR"
     gene            15083..15577
                     /locus_tag="VFC01_33990"
     CDS             15083..15577
                     /locus_tag="VFC01_33990"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZN84671.1"
                     /translation="MRGDGDGWVLSDTGAAYWGKHGAAGLLLRAPGRDGSPAVLLQHR
                     APWSHQGGTWGLPGGARDSHETAEQAAVREAHEEAGLPADLLTVRTTVITATAAGSHW
                     TYTTVIADAAELLETVPNRESSELRWVAEDDVVELPLHPGFAASWERLREVAATIPLL
                     QAEL"
     gene            complement(15549..16220)
                     /gene="thiE"
                     /locus_tag="VFC01_33995"
     CDS             complement(15549..16220)
                     /gene="thiE"
                     /locus_tag="VFC01_33995"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZN84672.1"
                     /translation="MHTPGERLASASLYLCTDARRERGDLAEFAEAALAGGVDIIQLR
                     DKGSPGEQQFGPLEARQELDALEVLADAARRHNALLAVNDRADIAVASGADVLHLGQD
                     DLPLAIARDIIGRRPLIGRSTHDATQTVAAVTEAVDYFCVGPCWPTPTKPGRPAPGLD
                     LVRTTAELATDKPWFAIGGIDEQRLPEVLAAGARRIVVVRAITGAADPTAAAQRLKAR
                     LAAAE"
     regulatory      16245..16355
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16346..17356
                     /gene="thiO"
                     /locus_tag="VFC01_34000"
     CDS             16346..17356
                     /gene="thiO"
                     /locus_tag="VFC01_34000"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HZN84673.1"
                     /translation="MPHGSLAVIGGGVIGLSVARRAALDGWTVRVHRSGERGASWVAG
                     GMLAPHSEGWPGEERLLQLGLASLAMWHDGFLDGLPADVVTAHESLVVGVDRADAADL
                     KTVGEWLAAQGHPVTLTSAARDIEPLLAQGIRRGFVAETELAVDNRAVVEALEAHCER
                     LGVQWAPPVVELAAVQADAVVIANGIDAPSLWPGLPIRPVKGEVLRLRWRRGCMPVPQ
                     RVIRARVHGRQVYLVPRADGVVVGATQYEHGRDTAPAVTGVRELLDDACAVMPSLGEY
                     ELAECAAGLRPMTPDGLPIVERLDERTLVAAGHGRNGFLLAPWTAERIAAELNVTVGA
                     SR"
     gene            17353..17550
                     /gene="thiS"
                     /locus_tag="VFC01_34005"
     CDS             17353..17550
                     /gene="thiS"
                     /locus_tag="VFC01_34005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003932867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HZN84674.1"
                     /translation="MKVTVNDEAVEIDEQTTVAALLESLGFPEKGIAVAVDWSVLPRS
                     EWQTALADGARVEVVTAVQGG"
     gene            17543..18301
                     /locus_tag="VFC01_34010"
     CDS             17543..18301
                     /locus_tag="VFC01_34010"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HZN84675.1"
                     /translation="MADSKLTIAGREFGSRLIMGTGGAANLSVLEEALIASGTELTTV
                     AMRRVDAESGTGVLDLLNRLGITPLPNTAGCRGAAEAVLTAQLAREALDTDWVKLEVI
                     ADERTLLPDAVELVRAAEQLVDDGFVVLPYTNDDPVLARRLEDTGCAAVMPLGSPIGT
                     GLGIANPHNIEMIVERAGVPVILDAGIGTASDATLAMELGCDAVLLATAVTRAADPAT
                     MAAAMAAAVTAGYLARQAGRIPKRFWAQASSPSL"
     gene            18298..19059
                     /locus_tag="VFC01_34015"
     CDS             18298..19059
                     /locus_tag="VFC01_34015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZN84676.1"
                     /translation="MSRYVALGSSMAAGPGIRPRADGAPFRAGRSARNYAHLVAEKLG
                     LDLVDVTYSGATTANVLTDPQHGAPPQVGALDGAEDLVTVTIGGNDVGYVPLLTAAGL
                     PRVVRFVPLLGGVVRELLDPTARDRALVDVAESLKEVGRTLRRRSPRAKVLFVDYLTL
                     LPPAGTPAPPLSDVDVALGRRVADTLERLTGEAAADTGCEWVRVADASREHHAWSAEP
                     WTSKPGLPLPGRPAPLHPNAKGMRAVADLVVAAAT"
     gene            19073..19981
                     /locus_tag="VFC01_34020"
     CDS             19073..19981
                     /locus_tag="VFC01_34020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN84677.1"
                     /translation="MIELAGLTKVFGRTRAVDDLTCSIEPGMVTGFLGPNGAGKTTSM
                     RLILGLDHPTAGTATIDGRPYRDLKEPLRTVGALLDARQAHPNRSARNHLRWIAATNR
                     IPADRVDEVLDMVGLTSVADRKAGTLSLGMSQRLGIAAALLGDPPVLLFDEPVNGLDP
                     EGIRWVRTLMRTLAGEGRTVFVSSHLLAEMANTADRLVVIGKGKLIASTTVAEFVGRS
                     GVDTVRVRSPQLDTLRRVLTDAGLTVDSDHGAALLVRGAAIEVIGDLAGRNAITLHEL
                     SAQRASLEEAYMQLTDEVTEYRAVSQ"
     gene            19978..>20452
                     /locus_tag="VFC01_34025"
     CDS             19978..>20452
                     /locus_tag="VFC01_34025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZN84678.1"
                     /translation="MSAVAVLDAERIKLSTTRSVLWTAAGVALLSFGLAALQASTAYG
                     AAPLPPEKAAMGVAVFGVPVLMVLAAMTVTGEYRSGMIRTTFMATPNRTLVLVAKAIV
                     AALFSAVYAAAMTVAAVVVARLMAPPLVGSNLSLVDACSAARPKLSSATPAAVHST"
BASE COUNT         3395 a         6775 c         6867 g         3415 t
ORIGIN      
        1 ccgtcgtcac cgatggccgg tgctggcccg cagaccgcaa cgccgtcacc gtcgcctgcc
       61 acacggtcgt gcacccatcc actgaaaggg caccacgaca tctcggcgac cgcggccacc
      121 accgcgtcaa cacgatcacc actatcgagc gcaccgccgc atcacagccc catcgaagac
      181 gtcaccaaac gactcaaact ggcagatacc gcgtcaatgc cgacgccgcc gcaacgccat
      241 taaccagagc ctccagatca tcgccggact ctgcagcctc aaaacccaca atcaattacg
      301 cgtcagctct tagcctggga tgtgtcgttt tcgcagacga cggtcggatg gctgctcctc
      361 ggcgctgccg cgctgacgtt cgccgtgggg ttcgcgtcgc gcacaatcat cggccgcaga
      421 cgaaacacca agggcgtgcg aaggctcgtg cacatgttcc ggaggaccgg tgtcgcgcgg
      481 gtgttgttcg ggcgattcga gggcgatgtg ctcgacgacg acgaactcga cagcatgatc
      541 ctgatgccca ccatcgtcgt cggttgtggg ctgtgcttga ccgcgatctt tctactcggc
      601 tacgaggtcc tcgcttagcg ccctacatcg cctcggccat cgcgatgtgt cgttcgaagc
      661 caagcgaatc tgccatcgtg cgatagcgat tcacgaagtc ttgataggca atgtcttcgc
      721 cacgggcccg gcacagcagc gcacgcagcc gcagtaacca gatctcgcac accgccgaac
      781 ccgcgtaatc cggcatattc accaggcggt cgatcgagcg ctgagcttct gcgatgtcgc
      841 cgtcggcgcg gcgttccagc agtgcctcca ccagatcggc ggtgggcacc acgcagagtc
      901 ccagctgctg catctggaac agcttttcca gggctgatcg catcatcgct attgcgcggt
      961 cgcgttcgcc gcgtccagct ctttctcggg cgatggccag ctcggcggtc ggtactagat
     1021 acaggcgagt acgctcgcgc agccacgtgt cgcggacctg cgtcaccagt tcgagcccgc
     1081 gccgacggtc cgcgagggtg gatcggtagg tcagcgcggc accgaaggac aacttgacag
     1141 tgctcagcgc ggtgtcgccg cttgatcttt cggcaatccg cagcgtctcc tcgatctcgc
     1201 gaagtgggaa ttcgtcagcc cgcagcacac cgttttcgat ggcgagcctg tttatgcctg
     1261 taatgagcaa tgcgtgggtt accggatcgc tgcgccgggc catcgcagat gcatcatcga
     1321 agtcctcccg ccacccagga cggcccaacc aagagcgccc gaagccgcgc aacgctatcg
     1381 ccgccgccag cggcgacccc atagcgaagt tggcgccctt ggtcgggtcg ccgtcggcca
     1441 ggtcgataac ggtctgcgac caatgaagga ggtcggcgat ctcgcaggct gcggctttga
     1501 tcaccatcac gccgtacgcc gctccaatgg tcagggccgg atcggcgatc gactcgagca
     1561 gcgccatctg ttcggacgcc agccgcgacg cgtcgcgcgt gtgcccagac cacatgagct
     1621 cactgaccgg cccagtcatg ccgatggcca gggaggcctt gtccccggcg cctgtgcaca
     1681 gatccctgag ttcctcgaag cgctcggagc tatctgattc ggctgctcgc caagagctga
     1741 cggacagcat ggtgcgagga gcgatgcgca tagcggtgcg gccgggatcg tcgtcgggca
     1801 gcgcgtcggc gatctggcgg gcacgctccc aactcacccg tgcagccgtg atgtcacggt
     1861 tggtcgacca agcgctggcg cgcatgtgcc agtcataggc ggcacgaagg tcgcctgctg
     1921 cttgcaggtg ctcggcgatc agcgccgcgc tctcatcaac agagtccggg tcacgctgct
     1981 ggatcgcgac ggcaaggcgc cggtgccact cggcgcgatc cgatttgagt tgtgattcgt
     2041 aggcaactgc gcggatcagc ggatggtgaa acgcatactg tgcgcgtggg gtgaatctga
     2101 cctgatcgat cagctcggct ccgatcagct cgtcgacgac cggctcgacc cccagcccgg
     2161 tcagtagttc ggtgtcgaaa cgcgagccga tcacggccgc tgcactcaat acgcgtttgg
     2221 ccggtgcatc caggcggtcg atgcgcgcac cgatggtcgc ctgcaaagtg gccggcacgc
     2281 tgacctcggt cacgggtgtt cgacagacat aagcgccgcg ctgcccgcgc aacactccgc
     2341 gctcggtgag gtctcgcacg atttcctcgg cgaagaacgg aataccgccg acccgcgaca
     2401 ccatgatttc gccgagctcg ccgacggacg ggtccgggcc gagcagctcc gcgagcaacg
     2461 tgacggtctc tgaatcatca agtggggcaa gcgctatcgt ttgggcgccg ggcacccgcg
     2521 tgagcgctcc ttcgtactcg ggccggcagg tgatcagcgc catcgacaag gttcgcggga
     2581 gggcagtcag aaagtcggtc agcatcgact cgctgaccgc atcgatccag tgtgcgtctt
     2641 cgatgatgta aagcgccggt gttgtccggg caagcgacgc ggcgttgatc aaggcggtca
     2701 atcggcgccg ccgggcgtcg ggatcgatgg ggggaagcgc cacatcggga tcggcagcgc
     2761 cgagcagatc atcgagcagc agcagatcct gcgggtcggc atcgggaatt cgttcgcgga
     2821 ttcgggcccg cgcgtccgtg ccgtccaggt ctgtcacgcc gatgactgtg cgcagcaggt
     2881 tcgaaaccac taggaacgga acgtctttgg cgtgcgactc gcagtaggtc cacaccacct
     2941 cgaacccgcg attcatcgcc agcgccgccg cttcacgggc cacccgagac ttgccgatgc
     3001 ccggcggccc cgccataccc accacatcac cgcgaccgcg gatcgcctgc tcgaccatgg
     3061 cctcgagggc ggccatttcc cagcgcctac ctaccagagt cgattccgtg cccgcagcgg
     3121 cgccgtgccc ggacacgacc gcaaacaacc gacgggcagg aactggttcg tcacggccct
     3181 ttatccgcac cagctcggga tccgcaagga ccgcggtttt gtcgactaag tgagcggtgg
     3241 attcggacag catcacccca ccggggggcg ccgccgactc catccgctgc gcgaagccaa
     3301 cttgttcgcc gattgcggtg taacccggta cgcccgaacc gatttcaccg gcgaccactt
     3361 gaccagaatt caggcccact cgcagctcta ggtcgatacc gtcgcggcgt tgcacctcgt
     3421 cggccaggcg cagagtctca ctttgtaggt cgagcgccgc cagacatgca cgaattgcgt
     3481 ggtcctccaa cgactttggc gcgccgaaca cggccatcaa gccgtcaccg gtgaacttgt
     3541 ccatcgtgcc gccataccgc tgcaccaccg ccgcaccgcg accgaccacg cctgccatga
     3601 tttcccgcag ccgctctgcg cccaccgctg cggcgatgtc cattgaaccc actacgtccg
     3661 caaacagcac agtcacctgt ttgaattccg cgcgtgctgg cgctgggagc gcgcctccgc
     3721 aggcgtcgca aaaccgagca cccgccctag cgggttcggc gccacacgat gtgcaaacga
     3781 tggccgctgc ggtcatccga gccccccgct ggtctcggaa tatttacgct gatcatgata
     3841 agcgggtaac cggctgccaa ggcggctttt tacaactgtc tcgaagcggc ctgctccgcc
     3901 gcctcgcgga tcgacccccg ccacaccggg ccatggccgg gcagcagtac gtcagtgtcc
     3961 agcaagccca acgcggccag gctgcgcaca cagccgtcct ggtcgtgatt gaacagatcc
     4021 ggcagaagct gcggtccgtc ccgtgttagc agcgggtgtc cggtgaccag cgcatcgccg
     4081 ctgacgagga cgccgtcgac cacaaacgag cagtgcccgc cggtgtggcc cggggtggga
     4141 atcgccatcg gtgtgcccgg caggccggcc gcgacgtcct cggtcagcgc ctgggtgctc
     4201 ggaatgccgt cgcgcaccat cccacctttc cgcgaaatcg ccgccgtcca cttcaagtac
     4261 cgcggctgcc ataggtgcgg aatgatgtcg gcgggcccgg cctgctcgag gtactcgcgc
     4321 ttggagtgcc cgacctcggc ggcgtggcag tacaccggtg tgccatgggt tttcgcgaac
     4381 cagatggccg tcccgagatg gtcgatgtgc gcgtgggtca acaggatcgc gcgcaggtcg
     4441 tcgacgccga gcccgagctg gcgcagcgag tcgaccacgt cgtcgcggtg gccggggaac
     4501 ccggcgtcga tcagcatgac gccgttgccg tcggtgacca atgtccagtt gaccaggtcg
     4561 gtgtacgcga agtgcacgct gtcggtgatg gcggtcagag ccggtgccat cagccgagtg
     4621 taggagcgtg gagtagaaac gaacacgtgg ctgaactcaa actcggatac aaggcgtcgg
     4681 cggagcagtt cgccccgcgg gaactcgtcg agctggccgt ggcggccgaa gaacacggca
     4741 tggacagcgc gaccgtcagc gaccacttcc agccgtggcg tcacgaaggc gggcacgcgc
     4801 cgtggtcgct ggcgtggatg gcggcggtgg gggagcgcac caagcgcctg atcctcggca
     4861 cgtcggtgct gaccccgacc ttccgctaca accccgccgt catcgcgcag gcgttcgcaa
     4921 ccatgggctg cctgtatccg gaccggatct tcctcggtgt gggcaccggc gaggctttga
     4981 atgagatcgc gaccggttac gagggcgagt ggccggagtt caaggagcgc tacgcgcggc
     5041 tacgcgagtc cgtcaagctg atgcgcgagc tgtggctcgg tgaccgggtg gatttcgacg
     5101 gcgagtacta ccgcaccaag ggcgcctcga tttacgacgt gcccgagggc ggtatcccga
     5161 tctacatcgc cgcgggcgga ccgcaggtgg ccaagtacgc cggccgcgca ggcgacgggt
     5221 tcatctgcac gtccggcaag ggcgaggagc tctacaagga caagctcatt cccgcgatga
     5281 aagagggcgc cgaggcggcg ggccgcaacc ccgacgacat cgaccggatg atcgagatca
     5341 agatctcgta cgatcccgat ccgacgctgg cgctggagaa cacccggttc tgggcgccgc
     5401 tgtcattgac cgccgaacag aagcactcca tcgatgaccc gatcgagatg gagaaggccg
     5461 ccgatgcgct gcccatcgag caggtcgcca agcgctggat cgtcgcgtcg gatcccgacg
     5521 aggccgtcga gaaggtcggc cagtacgtga cgtggggact gaaccacctc gtcttccacg
     5581 cgccggggca cgaccagcgc cgcttcctcg aactgttcca gaaggatttg gagccgcggc
     5641 tgcgccgact cggctgatac ggcaggcttt caggcgtgtc gaccaccgcg atctatatcg
     5701 cctcacctga gggcgatacc ggcaagtcga cgatcgcact gggcatcctg caccggttgg
     5761 cggccacggt cgcgaaggtc ggcgtgttcc ggcccatcac acggttgggc gaggaccgcg
     5821 actacatcct cgaactgcta ttggcccaca ccactgccgg ccttccctat gaggaatgca
     5881 tcggtgtcgg ctatcaacag ctgcacgagg atcccgatgg ggcgatcgcc gatatcgtcg
     5941 accggtacca ccacgtggcc ggccaatgcg acgcggtgtt gatcgtcggc agcgactaca
     6001 ccgacgtcgc cacccccagc gagctgtcca tgaacgcgcg gattgcggtc aacctcggcg
     6061 cacctgtggt gctcgcggta aaggccaagg gccgcacccc cgaacaggtc gcccaggtcg
     6121 tcgatctgtg cctgttcgaa ttggatgccc aacacgccca caccgcggcg gtggtggcca
     6181 accgctgcga ccccgcgcat atgaccgcgg tcgccgaggc gttgaaaccg ttgggaccca
     6241 agacatatgt gctgcccgag gagccgctgc tcgtcgcccc gtcagtggcc gagcttggag
     6301 cagcggtcga gggcacgctc ttgcgcggcg acccggcgct gttgacccgc gaagcgatgg
     6361 acgtcctggt ggccggcatg acggccgagc atgtggtgga gcggctgacc gagggcgtgg
     6421 ccgtcatcac ccctggcgac cgttccgacg tcgtgctcgc cgtcgtgagt gcccatgcgg
     6481 ccgaaggctt tccgtcgttg tcctgcgtga tcctcaacgg cggcctcgaa ctgcatccgt
     6541 caatcgcgtc tctggtcgcc ggcctggggc tgcggctgcc gatgattgcg acccgcttcg
     6601 gcaccttcga aaccgccagc cgggttgcat cggcgcgcgg ccgggtcaca gcatcctcgc
     6661 aacgcaagat cgacaccgcg atcgcgttga tggacaccca cgtcgacaca gcagatctgc
     6721 tggcgcaact ggcgatttcg attccatcgg tcgtcacccc gcagatgttc acctaccaac
     6781 ttcttgaccg cgcacgttcc gtccgcaagc gcattgtgct gcccgaaggc gaggacgacc
     6841 gcatcctcaa agcggcagga cgactgttgc agcggagcgt cgctgacctg accatcctcg
     6901 gtgacgagcc gcaaatccgg gcccgcgcag ccgaactcgg tgtcgacctg tcgaccgcca
     6961 aggtgctcaa cccccgcacc agcgacctgt gcgacaagtt cgccgagcag tacgccgagc
     7021 tgcgcaagcg caaaggcgtc accgtcgagc aggcccgcga aatcatccat gacgtctcgt
     7081 acttcggcac catgctggtg cacaacaaca tggtcgacgg catggtgtcg ggcgcgacgc
     7141 ataccacggc gcatacggtg cggccggcgt tcgagatcat caagacgctg cccgacgttt
     7201 caacggtgtc cagcattttc ctgatgtgcc tggccgaccg ggtgctggcc tacggcgact
     7261 gcgcgatcgt gccggacccg acgtccgagc agctggccga catcgcgatc tcgtcggcac
     7321 gcaccgccgc ccagttcggc atcgatccgc gcgtggccat gctgtcctac tcgacgggca
     7381 ccagcggcac cggcgccgac gtcgacaaag tcagggctgc aaccgaattg gtccgcaagc
     7441 gcgaacccga tctgctcgtc gaggggccaa tccagtacga cgccgccgtg gacccgacgg
     7501 tcgccaaggc caagatgccc gactccaagg tcgccggccg ggcgaccgtg ctgatcttcc
     7561 cggacctcaa caccggcaac aacacctaca aagcggtgca gcgaagcgcg ggcgctatcg
     7621 cgatcggccc ggtcctgcag ggtctcaaca agcccgtcaa cgacctgtcg cgcggcgcgt
     7681 tggtcgaaga catcgtgaac accgtcgcga tcacggcgat ccaggcgcag agcctatgag
     7741 ccgcagcgtt ttggtgctca actcgggctc ctcatcggtg aaattccaac tgatcgaccc
     7801 ggattcgggc gcctcgctgg ccgacggaat cgtcgagcgg atcggcgagg acgaatcggc
     7861 ggccagcctg acggtcggcg agaacgaggt gacgcgcgac gatcgggtcg cagaccacga
     7921 agcggcgctg cgcaccgcgt tcgagatgtt cgacgaggcc ggcacccacg tggacactgt
     7981 cgggctcgtc gcggtcgggc accgggtggt gcacggcggc ccggatctgt accggccgac
     8041 gctcgtcgac gatgccttga tcgccaagct cgaggacctg gctccgcttg cgccgctgca
     8101 caatccgccc gcggtgcttg gtatccgtgt ggcgcgcaag gcgctacccg atctgccgca
     8161 cgtcgcggtg ttcgacacgg cgttcttcca cgatcttccg cccgcggccg cgacgtacgc
     8221 catcgatcga gacaccgccg agcggtggca catccgtcgg tacgggttcc acggcacgtc
     8281 gcaccagtac gtcagcgagc aggccgcggc gttcctcgac gtgccgctgg tcaagctcaa
     8341 ccagatcgtg ctgcacctgg gtaacggcgc atcggcgtcg gcgatcgtcg gcggacgccc
     8401 cgtggacacc tcgatgggcc tgaccccgtt ggaggggctg gtgatgggca cccgctccgg
     8461 tgacatcgat cccggcgtgg tcacctacct gtggcgcacc gcgaagatga gcgtcgagga
     8521 catcgagtcg atgctgaacc gcagatcggg tgtccgaggc ctcggcggcg agatcgactt
     8581 ccgcgtgctg caccagcgca tcgaatccgg cgatgagaca gcgcaattgg cctacgacgt
     8641 gtacatccac cggctgcgca agtacatcgg ggcctatctg gcgctgctcg gcaccgcgga
     8701 cgtgatcacg ttcaccgcgg gggtcgggga gaacgacgca gcggtacggc gcgacgctct
     8761 ttccggtatg gcggcgctgg gcattgagct cgatgagcac ctcaatgcga gcccggcaag
     8821 ggcggcccgc cggatctcgg ccgacaagtc gccgacgacg gtgctagtgg tccccacaaa
     8881 cgaggagctg acgatcgcgc gggcctgcgt tgaagtgatt tgacggccgg cccgcaggaa
     8941 aggcgatacg gtctgcgtgt ggcgacaccg gaccgtgaaa gtgtgctcaa cgagctggaa
     9001 gtcctcgcca ccctcgaaca cgcactgatc gtcgagttcc tgtcgattgc gtgcgcgctc
     9061 ggccttgatc tcgacgccca ggacggcgga ccgacgacgg accggggacg ggacgccgcg
     9121 tctgctgcgt ccgtacgggc attcactgag atgtttcact tcaagtgcgt caaccaggcg
     9181 ctggtcggct ctgggcggcc gccgcggatg gatcgcgccg aggaagtcac gacgacatcg
     9241 ggacaggtat cgccaattcc cccggaccaa gcgggattcg aacgactgtt ggagcatgag
     9301 cgctcattgg cggccgaagt tgatgaccgt tatgccgaag ttgccgcggt actcactacc
     9361 gtgttcgacg gcgatgaact caatgagtgg cgcgacgttc ttgttgacaa cgccaaccac
     9421 gtcggcgacc tagccgacct acagaccgcg ctggacggtg tggcgcccgc ggacttcctg
     9481 cgcgccaccc gccgaactcc gaacgacggg ttcgaagagc gcatgctcga tgaatcgccg
     9541 gatctacgga gtgatcgtgc gtgcgctccg gggcagtttc gatccggatt tcggtgtcgc
     9601 tgagtccttt cgggacatcg cgacatcgtt gatgacgccc tttggacgac tcgaatcacc
     9661 ttctggtgca acggggcctg ctgccgccgt tcccgcttgt ctgacgacgc gccgacgacg
     9721 gcgctagtgg ttcccaccaa cgaggagctg gcgatcgcgc gaggccgtct ccaggcgatt
     9781 gacggctgaa tcctgcagcg gcagtaagtt ttcgggatca cagatccgca ggaagcgtcg
     9841 cagctgcggt ccggcgacca gcacccacga gacgccgtat cgggcacaga tcacgttgac
     9901 gttgtgcagc gccgcgaatc ctgcggcgcc gaagaattcg atgaccgaca gatccagcac
     9961 caggcgtgtc gatccgacga cctggcgttc gacgtacgca gcgagcgcac ggttgtttgt
    10021 ggcgtcgact tcgcccctga cagtgacgag aacggtggtg tcctcgacgt ggcaagcgga
    10081 gaagatcgct cgatggtgct cttcgcgccg gtcgagcgat tgcggctcag cgcggaacag
    10141 acggcatgta cctacagcgg agttcatgga gacctccctc agtcgaaagt gcgggtgccg
    10201 tcaggaccgg ccctacttgt ttgaaagcca tgcacttggg agttcagccc ccgcgcgctc
    10261 ttgacgtcac cagtcagctg ttacctcaac ctgaggggca gggtactacg aaactcgtag
    10321 cttgaccatg gctttttcgg atgattccgc cctgtccagg gcaaagaccg cggatcagaa
    10381 ggtgctcgtc ggccgcacgc tgttggccag gtcgatgagc gcgtagcggt gcgcctgtgt
    10441 gggcgctacc cgggccaggc tgcgcaacga cgcctcgaca cccaactgca gcccgtgttc
    10501 ggtgaacggg aagccgagga tgtggttggt gcttgcggtg ttgtcggcca gccagtccat
    10561 cgcggtgccg agcacgagcg cacggatctg cagaactctc ggttcggtgt cgggcagtgc
    10621 ctcgacgcgg cgggccgcgt cgcggatctg ttgttcggtg atctcgctca tcgaccgacc
    10681 cgacagcagc gtaaccgcac tcgtcagccg cgcggtcgtg aaatgccttg acgtggctgg
    10741 cacttcgtca agagtctgta ccgcactgtc gcggtcgcct tccgcggact gggcgcgggc
    10801 caggccgaag cccgccgaga tgacgccgtt gtcggtgcac cacaccgtct tgtagaactt
    10861 gcgttcgtcg gcgctacccg ccagctcggc tgtcgcggcc agcgccagct tgggtgccag
    10921 ctcgccgggc agcgtgtcca gcacttcggt gaaatgcttt gacgccgatt cgaagtccgc
    10981 cgaaagcatc tcggcgacag cacggaacca caccaaccgc caccgccagc cgacccggcc
    11041 cgcgaggtcg tcgagcttgc gggtggcctt cgcgacatcg ccgaggtcca gcagcgcgcg
    11101 gacctccatc agcggcagct cgaccgactc ggacaggtcg atgccttcgg aatccagcgc
    11161 gccatgccgt gcagcacgca gctggtccaa cgtctgcacg ggctggctca acacgctcgc
    11221 cgatagcacg gccgctccga cgtcggccgg gtcgaccagc ggcaccggga gtgccttgat
    11281 gatctcctgc gccgtcaact tctccgaatg cacctggccg tcgagataga catcggtgtg
    11341 cgcgaccagc agatccaccc cgaacgtcga tcgcgacggg gagaacaccg tcgacaggcc
    11401 cggccgcggc accccggtgt cctgtgcgac gacttcgcga agcacgccaa gcagctgtgc
    11461 cgacatctct tcggcactgc cgaacctgcg ccgcgggtcg ggatcgatgg ctcggcgcag
    11521 cagccggctg tacgagtcgt actccctgag caccgggtcg tcctcgggca ggccgtcgac
    11581 gtagcggccc ttgcgggtcc gcaggttcag cgtcagcgcc gcgagtgtgc ggccaacggt
    11641 gtagatgtcc gtctgtacgg tcgggccggt gcggacaatc tccggcgcct ggtaacccgg
    11701 tgtgccgtag aggtatccga acgagttgac ccgcgacacc gcaccgaggt cgatcagctt
    11761 gagctgctcc tcggtcacca tgatgttctc cggcttgagg tcgttgtaga tcagcccgat
    11821 cgaatgcaga taacccatcg cgggcaggat ctccatcatg aagccgatgg cctcggccac
    11881 gggcagccgg tcgcccttgg cctgcttcag cgatgtcccg ccgacatact ccatcacgat
    11941 gtagccgacc gggttgccgt gcttgtcgtc gtgctcgacg aagttgtaga tcttcacgat
    12001 cccgggatgc gttacctcgg cgaggaattg gcgctcggcc atcgcgatgg tctgcgcctc
    12061 ggcgtcaccg gagtgcacga gtcctttgag gacgacgggg cgatcgttga cgttcttgtc
    12121 gtaagccagg tacacccagc ccagtccacc gtgtgcgatg cagcccttga tcacatactg
    12181 ctctgcgacc tcgtcgtccg gattcagttg cggcaggaag gaatatgcgc tgccgcagtg
    12241 tgggcaccag ccttcggaga gggcccgtcc gtcgggtgac gaccggccga ccgggcggcc
    12301 gcagttccag cagaaccgct tcgattcggc caccaccgga ttggtcatca gcgcttcgag
    12361 cggatccttg gccggcaccc gggggatctc caccagacca ccgccgaggc ggcgggtcgg
    12421 cgacagtgcg cgcgtcaccg tggtcaagtg atcgtgcggt tcggtgtctt ccgtgccgac
    12481 cgacgtgctg tcgacatcgt catccagacg tggacggaag atcgcctgag tggccatcgg
    12541 gcgcatcgtc gacatggagt ccatgccgag atcgtcgaag ctggccggtt gggtgccggg
    12601 gctctcgtcg tagtctttcg cctcggccat cagtccgaat acctcgcgac tggcggcgcc
    12661 ggagccggac cgagcaccgt taaccacttg cggtacaacg tgtaccaggt gccgtcgcgg
    12721 cggatccgct ccagcgtgcc attgacgaac cgcaccaatc cggtgttctt caggttcaca
    12781 ccgatcccgt agggctcttc gttgaggctg ggcccgacga tgtgaaggta cgggtcttgc
    12841 gataccaggc cggccaggat cgagtcgtcg gtgctgaccg cgtcgacctg ccgctgctgc
    12901 aacgccacta ggcagtcggc ccacgtcacc acgccgacga tcatcggggg cgggctgatc
    12961 tgttgaatcc ggtcgagcga cgtcgtgccc ttcgcgacgc acacccgctt gccggacaga
    13021 tcggacggct gggagatcga cgaatcccgc gcggccagaa tccgttggtt ggcgttcagg
    13081 tacaccgtcg agaaaccgac ctgcttcttg cgctcacagg tgatggtcat ggtcttgacg
    13141 acgacatcga cctgattgtt ctgcagcgcg gcgatgcggt cggccgacga caggatgcgg
    13201 tactcgacct gcgacgggct gccgaagatg tcacgcgcga tctcgccggc gatgtcgacg
    13261 tcgaagcccg tgatctcgcc ggtgatcgga tcgcggaagg aaaacaggtt gctgccgatg
    13321 tcgagcccga cgatcagcct gccgcggttg cgaatggtgg cgacggcggc gtccgcttcg
    13381 gctttgttgc tgaacggccg caggctcgcg gtgcgattgc agtcgccctc gtcttcgggg
    13441 gctttcggag gcgccggtgg cagctcctgc atgcctgcgg gtgtgggtgg cgcgagtgtg
    13501 acgccgggtg acggcatcac cgcagcgccc tgcgcgcaac cggtcagcac cgccgcggcg
    13561 gcgagcagaa atacggggat tgcgcccccc gtccgtctgt tcttcgcgcg agcgctcatc
    13621 accggtactc actcagcctt ggccacaacc cgagcgccac cgccaacgcg gcaacgaccg
    13681 acagcaccgc ggcgccgacc gtcgcacccg acagcacccg gcgggcgttg agaatgtcgt
    13741 tgcgcaactg gctgcggctt tccccgatgc ctttcgtcag cgccgtgtcg agcttgtcga
    13801 acgcgggggt cgagtcgtct tcgccggtgc ccagcgccac ctgggtagcg gcctggtaat
    13861 tgccgaccgc gatgtaggcg ttgatccggt cgtcggcggc ccgccaccgc ttcagcagtt
    13921 gttcggcgtc ggccaggtcg gccttgtcga tcgcgtcgga gcgagacagg tagtcggaca
    13981 gttgctgctg catcgagtcg atgcgctggt agtaggactg cttgcgcacg tcctcgtcac
    14041 cgcgccggat caacgacagc gtctcgtcgg cacgcgcctg ctgcgcggtg atcgcgaggc
    14101 tggtcacggt cttgagtgac tcagccgcag tgtctttcgc gctgcggctg tccgcagtcg
    14161 agatgacgag ggcggtgccc acccatatca acataatcag cacggcaagg ccgcccgcga
    14221 caaagccgat attcacccgg cggcgagtcc ggcgcgcgag ccagcgattt gcgaacgcac
    14281 cgaacaacag cgtcgcgagc accacgagaa tgaccggccc gggaattctc gccgacgcgg
    14341 tcgtctccgc gtccactcgc gccgacgtct cttcgtagag ccgctgcgcg tcgggcagca
    14401 tctgcgtctg catcaacgac gacgcctcgg acaggtacga cgagccgacc gggttgccgg
    14461 cgcggttgtt ggtgcgggcc gtttcgacca gcccggtata tacggacagc tgggcgttga
    14521 cccggccgag aagttgcacc atcggctcgt cggtcagccc gctggacgcg cgggtgaccg
    14581 cgaccgacgc gtcggtgatg gcctgctcgt agcgctgccg gacgtcgcgt ggttccgagc
    14641 ccgcgatgaa cgcggtcgcc gccgcggcgt cggccaccga gagggttgtg tacagctggc
    14701 cggccgcgaa cgacagcggc tcggtgtgat taagaactgt tgtgagcgcc tcttggcggt
    14761 cgttgatcgt cgtcgacgtg gcaaacgcgc tggcaatcac gagcgccgac agcacaaccc
    14821 cgatggtcag gatccggccc ggagtggtcc ataggaacca ccagcgtggg tgcgctggtg
    14881 ttgccggcga ccgcgacgca agtggctcgg tcgacgggtg cgccaactcc acagtcacgt
    14941 gtgcgcgaac ctcatctttt cgctgctctc cggctatcgc cccgaccgtt taccactcta
    15001 taaaagaatt ctaagagttt cacacgtgac gcgtgggtat tcgaggtgca cgtctttcga
    15061 ccgtgttcct taagctgagg gcgtgcgagg cgacggagac ggctgggtgt tgtctgatac
    15121 cggcgccgct tattggggca agcacggggc ggccggtctg ctgctgcgag cgccggggcg
    15181 cgacggctca cccgccgtgc tgttgcaaca ccgcgcgccg tggagtcatc agggcggcac
    15241 gtggggtctg ccgggcggcg cccgcgacag ccatgagacg gccgagcagg ccgccgtccg
    15301 cgaggctcac gaggaagccg ggctgcctgc cgatctgctg accgtgcgca ccaccgtcat
    15361 caccgcgacc gcggccggca gtcattggac gtatacgacg gtgatcgccg acgccgccga
    15421 actgctcgag acggtgccca accgcgagag ctcggagctg cgctgggtcg ccgaggacga
    15481 cgtggtcgag ctccctttgc atccggggtt cgccgccagc tgggagcggc tgcgtgaagt
    15541 ggcggcgact attccgctgc tgcaagccga gctttgaggc gttgcgccgc ggccgtgggg
    15601 tccgccgcgc cggtgattgc gcggacgact acgatgcggc gtgcgccggc cgcgaggact
    15661 tcgggcagcc gctgttcgtc gatgccgccg atcgcgaacc acggcttgtc cgttgccaat
    15721 tcggccgtgg tgcggaccag atccagaccc ggcgccggac gaccgggttt ggtgggggtt
    15781 ggccagcacg ggccgacgca gaagtagtcg acggcttcgg tcaccgcggc gacggtttgt
    15841 gtcgcgtcat gcgtcgaccg accgatcagg gggcggcggc cgatgatgtc gcgggcgatc
    15901 gccagcggca ggtcgtcctg gcccaggtgc aacacgtcgg cacccgaggc caccgcgatg
    15961 tctgcgcgat cgttgaccgc cagcagcgcg ttgtggcggc gtgccgcgtc ggcgagcacc
    16021 tccagtgcat ccagctcctg ccgcgcctcg agcgggccga actgctgctc tcccggtgaa
    16081 cctttgtcgc gcagctggat gatgtcgact ccgccggcca gcgccgcctc ggcgaattcg
    16141 gccaggtcac cgcgctcacg gcgggcgtcg gtgcagagat acagcgacgc cgaggccagc
    16201 cgttcaccgg gtgtatgcac acggcgacgg taacggctac cgtggatgga caacacggga
    16261 gtccctggac cgcggactga gagtaggcgc gcccgccttt accgtcacac ctgatccggg
    16321 tcatgccggc gaagggagga ggaagatgcc ccacggatca ctggccgtca tcggtggcgg
    16381 cgtcatcggg ctgtccgtcg cgcggcgcgc cgcactggac ggctggaccg tccgcgtgca
    16441 cagaagcggc gaacgcggcg cgtcgtgggt cgccggtggc atgctcgccc cgcacagcga
    16501 aggctggccc ggcgaggagc gtctgctgca gttgggtttg gcgtcgctgg ccatgtggca
    16561 cgacggattt ctcgacgggt tgcccgccga cgtcgtcacc gcgcacgaat cattggtggt
    16621 cggtgtggac cgcgccgatg ccgcagacct gaagacagtc ggggagtggc tggccgccca
    16681 gggccatccc gtcacgctga cgtcggccgc ccgcgacatc gaaccgcttc tggcccaagg
    16741 cattcgacgc ggattcgtcg cggaaaccga actggccgtt gacaatcggg ccgtcgttga
    16801 agcactggag gcgcactgcg agcggctcgg cgtgcagtgg gcgcccccgg ttgtcgagct
    16861 ggctgcggtg caggccgacg cagtcgtcat cgcaaacggc atcgacgcgc cttcgctgtg
    16921 gcccgggttg cccatccggc cggtgaaggg cgaggtgctg cggctgcgct ggcggcgcgg
    16981 ctgtatgccg gtgccgcagc gtgtgatccg ggcccgtgtg cacggccgcc aggtgtatct
    17041 ggtgccgcgc gccgacgggg tggtcgtcgg cgccacccag tacgagcacg gccgcgacac
    17101 cgcaccggct gtgacgggcg tgcgtgagct gctcgacgat gcgtgcgcgg tgatgccctc
    17161 gctgggggag tacgaactcg ccgaatgcgc cgccggtttg cgtccgatga caccggacgg
    17221 gttgccgatc gtcgagcggc tcgatgagcg cacactggtc gctgcggggc acgggcgcaa
    17281 cggatttcta ctcgcgccgt ggaccgccga gcggatcgcg gccgaactga acgtcacggt
    17341 gggagcaagc cgatgaaagt gaccgtcaac gacgaagcgg tcgagatcga tgagcagacg
    17401 accgtcgcgg cgctgctgga aagccttggc tttccggaga agggcatcgc ggtcgccgtc
    17461 gactggtcgg tgctgccccg atcggaatgg cagaccgcgc tggccgatgg cgcgcgggtg
    17521 gaagtggtaa cggcggtgca gggtggctga ttccaagttg acgatcgccg gtcgtgagtt
    17581 cggctcccgg ctgatcatgg gcaccggcgg cgccgcgaac ctgagcgtgc tcgaggaggc
    17641 gctgatcgcg tcgggcaccg agctgaccac cgtcgcgatg cgccgcgtcg acgcggagag
    17701 cggcaccggc gtgctggacc tgctgaaccg gctcggcatc acaccgctgc cgaacaccgc
    17761 cggctgccgc ggggccgcgg aggctgtgct gaccgcacag ctcgcccgtg aagcgctgga
    17821 caccgactgg gtgaaactcg aggtcatcgc cgacgagcgc acgctgctcc cggacgccgt
    17881 cgaattggtc agggcggcag agcaattagt cgacgacggt ttcgtcgtgc tgccgtacac
    17941 caacgacgat ccggtgctgg cgcgccggtt ggaggacacc ggctgcgcgg cggtgatgcc
    18001 gctgggctcg ccgatcggca ccgggcttgg catcgccaac ccgcacaaca tcgagatgat
    18061 cgtcgagcgg gccggcgtgc cggtgatcct cgacgccggc atcggcaccg ccagcgatgc
    18121 gacgctggcg atggagttgg gctgcgacgc agtgctcttg gccaccgccg tgacgcgcgc
    18181 agcggaccca gcgacgatgg ccgcagcgat ggccgccgcc gtcaccgccg gatacctggc
    18241 ccggcaggcg ggccggatcc cgaagcggtt ctgggcgcag gcctccagcc cttcgctatg
    18301 agtcgctatg tcgcgctggg cagttcgatg gcagcgggcc ctggtattcg tccgcgcgcc
    18361 gacggcgcgc cgtttcgggc cggtcggtcg gcgcgcaact atgcgcatct agtcgccgag
    18421 aagctgggtc tcgatctggt cgacgtgaca tattcgggtg ccaccaccgc caacgtcctc
    18481 accgatccgc agcacggggc gccgccacag gtcggtgcgc tcgacggcgc tgaggatttg
    18541 gtgaccgtga cgatcggagg caacgacgtc ggctacgtgc cgctgctgac ggcggcggga
    18601 ctgccgcgcg tggttcgctt cgtgccgcta ctgggcggcg tcgttcgcga attacttgat
    18661 ccgacggccc gcgatcgggc gctcgttgac gtcgccgagt cgctgaagga agtgggccgc
    18721 accctgcgcc ggcgatcacc gcgggcgaag gtgctgttcg tcgactacct cacgctgttg
    18781 ccgcccgccg gtacgccggc gccgccgctg tccgatgtcg acgtcgcgct cggccgccgg
    18841 gtcgccgaca cgctcgagcg gctcaccgga gaggcagccg ccgacacggg atgtgaatgg
    18901 gtgcgggtcg ccgatgcgag ccgcgagcat cacgcctggt cggccgagcc gtggacatcg
    18961 aaaccggggt tgccgttacc gggcagaccg gcgccgctgc accccaacgc gaaaggcatg
    19021 cgagcggtcg ccgacctcgt cgtcgccgcg gcgacctgac atcatggagc cggtgatcga
    19081 gctggcgggc ctgaccaaag tctttggccg aacacgtgcc gtcgacgacc tcacctgttc
    19141 gatcgaaccc ggaatggtca ccggcttcct cggccccaac ggcgcaggca agaccacgtc
    19201 catgcggctg attctcgggc ttgaccaccc gacggccggc accgccacca tcgacggccg
    19261 gccctaccgt gacctcaagg agccgctccg aaccgtcggg gcgctgctgg acgccagaca
    19321 ggcgcacccg aatcggtccg cgcgcaatca cctgcgctgg atcgcggcga ccaaccggat
    19381 ccctgccgac cgggtcgacg aggtgctcga catggtcggt ctgacgtccg tggcggacag
    19441 gaaggcgggc acgctgtcgc tgggcatgag ccagcgcctc ggcatcgccg ccgcgctgct
    19501 cggcgatcca ccggtgttgc tgttcgacga gccggtcaac gggcttgacc cagagggcat
    19561 ccgctgggtc cgcacgctga tgcgcacgct ggccggcgag ggtcgcacag tgttcgtatc
    19621 cagtcacttg ctggccgaga tggccaacac cgccgaccgg ctcgtcgtca tcggcaaggg
    19681 caagttgatc gcgtcgacga cggtggccga gtttgtcggc cggtccggcg tcgacacggt
    19741 ccgggtgcgc agcccccagc tggacacgtt gcgtcgggtg ctgaccgatg ccggactcac
    19801 ggtggacagc gaccatggcg ccgcgttgct ggtgcgtggc gcggccatcg aggtcatcgg
    19861 ggatctcgcg ggccgcaatg cgattacgct gcacgagctg agcgcgcagc gggcctcgct
    19921 cgaggaggcc tacatgcaac tcaccgacga ggtcaccgaa taccgggcag tgagccagtg
    19981 agcgcggtcg cggtgctcga tgccgagcgg atcaagctgt cgacgactcg gtcggtgctg
    20041 tggacggccg ccggggtggc gctgctcagc ttcggtctgg ccgcactgca ggcgtccacg
    20101 gcatacggtg cggcgccgct gccaccggag aaggccgcga tgggggtagc ggtattcggc
    20161 gtaccggtgc tgatggtgct tgccgcgatg acggtgaccg gtgaataccg cagcgggatg
    20221 atccgcacga cgttcatggc cacgccgaat cgcacactgg tgttagtggc gaaagcgatt
    20281 gtcgcagcgc tgttttcggc tgtgtatgcc gcggcgatga cggtcgccgc agtggtggtc
    20341 gcgcgcctga tggcccctcc gcttgtcggc tcgaacctgt cgttggtgga cgcctgcagt
    20401 gcggccagac cgaagctgag cagcgccacc ccggcggccg tccacagcac cg
//
LOCUS       DASRJA010000753         3860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3300315,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000753 DASRJA010000000
VERSION     DASRJA010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3300315"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFC01_34030"
     CDS             <1..194
                     /locus_tag="VFC01_34030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HZN84679.1"
                     /translation="VLVLFMVIDGFLVGRKVNKLVDQKFPDNTESGWKLGFYAASRAS
                     QLRRMRAPRPQVNRGDKVA"
     gene            201..734
                     /locus_tag="VFC01_34035"
     CDS             201..734
                     /locus_tag="VFC01_34035"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HZN84680.1"
                     /translation="MRTLVLGGIRSGKSQWAETAIAESTGAGQPVRYLATGAIPDGDA
                     DWSARIAAHRARRPPHWSTIETVDVPTQLRSDAATPTLVDDIGGWLTAAMDRRGAWTT
                     GSVAADVADLLDAVEAFPSPLALVSPEVGLTVVPHTASGRRFADELGSLNQRLAALCD
                     RVVLVVAGQPLTVKAAS"
     gene            731..1768
                     /gene="cobT"
                     /locus_tag="VFC01_34040"
     CDS             731..1768
                     /gene="cobT"
                     /locus_tag="VFC01_34040"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HZN84681.1"
                     /translation="MTGFEVTAPDADVAAAARARQDRLTKPLGSLGRLEDLSVWVASC
                     QGVCPPRQFQRPRVVVFAGDHGVTSAGVSAYPAEVTAQMVANFDAGGAAINVLAEAAG
                     ASVRVVDIAVNAAEPLSAAIGAHKVRRGSGNIAVEDALSAEETAAAIEAGRAIADEEV
                     DSGADLLIAGDMGIGNTTAATTLIAALTNSEPVAVVGRGTGIDDAGWARKTAAIRDAL
                     YRARGLSADPVGLLRVCGGADFAAMAGFCAQAAVRRTPVLLDGVVVTAAALVADRLAP
                     GARQWWQAGHRSTEPAHALALQQLDLEPIIDLRMRLGEGTGAAVALPVVRAAVAALSS
                     MATFDEAGVTA"
     gene            1765..2490
                     /locus_tag="VFC01_34045"
     CDS             1765..2490
                     /locus_tag="VFC01_34045"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HZN84682.1"
                     /translation="MIRSVTGALAFATVLPVRGSAGVGRGVITALPVVGVALGAVAGG
                     VVWASSWAFGAGSPLGGALAVTVLLIATRGLHIDGLSDTADALGCYGPPDRALQVMRD
                     GSAGPFGVAAVVVAILVQVLAFAQLSIVAVVVAVTAGRVAAVAACRRAVPAAQGSSLG
                     ARAAGTQPAAVVLLWIIGVAVLAVLADARRWQGPLAVLVALVCAGALVAHCVRRFGGI
                     TGDVLGAAIEVTTTVTAVALAIR"
     gene            2637..3422
                     /locus_tag="VFC01_34050"
     CDS             2637..3422
                     /locus_tag="VFC01_34050"
                     /inference="COORDINATES: protein motif:HMM:NF020962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HZN84683.1"
                     /translation="MTRTIPPSLAPILEQLELNGDELVTTERLDELVRATGIDTPTRV
                     VAARLRDRGWLLTTGQRGVWEFAPAAVAGPFSRGGPTRLLRSLLARRNVTCGLTFQAA
                     AWAIGAADRAPMRIEVAVANERDVRWMPDGLDVSIFDPQLPYRTAKGVPTLQPASVLT
                     HMATTPARVRSWSSAAEWLPDIAAEITIADALTELGGRPATVGARLGYLLQGLRPDVA
                     EQITRPSTKTWFGPRRKLVRNDERWKIADTILPFDPRDFEPVT"
     gene            3419..3682
                     /locus_tag="VFC01_34055"
     CDS             3419..3682
                     /locus_tag="VFC01_34055"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HZN84684.1"
                     /translation="MTSQQISITAGWIARHTPTGAGAGGRDAAVIDIAQDLLLRDLHT
                     RDALEAIVFKGGTALRKLYAGNQGRFSVDLDFSLARANGAYSA"
BASE COUNT          587 a         1226 c         1374 g          673 t
ORIGIN      
        1 tggttctggt gctgttcatg gtgatcgacg ggttcctcgt gggccgcaag gtgaacaagc
       61 ttgtcgatca gaagttccct gacaacactg aaagtggttg gaagctcggc ttttacgccg
      121 ccagccgcgc ttcacagctg cgccggatgc gcgcccctcg gccgcaggtc aatcgcggcg
      181 acaaggtcgc ctgaccgccg gtgcgcacgc tggtgctcgg gggtatcagg tcgggtaagt
      241 cgcagtgggc cgagacggcg atcgcagagt cgacgggcgc cggccagccg gtgcgctatc
      301 tggccaccgg cgcgattccc gacggtgacg ccgactggtc ggcgcgtatc gccgcccacc
      361 gggctcgccg cccaccgcat tggtcgacga tcgaaacagt cgatgtacca acgcaattgc
      421 gctccgatgc tgccacaccg acgctcgtcg acgatatcgg cggctggctg accgcggcga
      481 tggaccgccg tggcgcgtgg accacgggct cggtggcggc cgacgtggcc gatctactcg
      541 acgcggtcga ggcgttccct tcgccgctgg cactcgtcag cccggaggtg ggcctgaccg
      601 tggtgccgca cacggcgtcc ggtcgtcggt tcgccgacga gctcggcagc ctcaaccagc
      661 ggctggccgc gctgtgcgac cgggtcgtgc tggtggtcgc cgggcagccg ttgacggtca
      721 aggcggcgtc gtgacgggct tcgaggtcac ggcgcccgac gccgacgtgg ccgccgcggc
      781 gcgcgcccgt caggaccggc tgaccaagcc gctcggctcg ctgggccgcc tcgaagacct
      841 gtcggtctgg gtggcgtcct gccagggggt gtgcccgccg cggcagttcc agcggccccg
      901 cgtcgtcgtg ttcgccggtg accacggcgt cacctcggcg ggggtctccg cctatccggc
      961 agaagtcacc gcgcagatgg tcgccaattt cgacgcgggc ggcgcggcga tcaatgtgct
     1021 cgccgaggcg gcaggggcaa gcgtgcgggt ggtcgatatc gcggtgaatg ccgccgagcc
     1081 gctgtccgcg gcgatcggcg cgcacaaggt gcggcgcggc agcggcaaca tcgcggtcga
     1141 ggacgcgctc agcgcggagg agacagccgc ggcgatcgag gcgggccgtg cgatcgccga
     1201 cgaggaagtc gattcggggg cggatctgct gatcgccggc gacatgggga tcggaaacac
     1261 cacggccgca accactttga tcgccgcgct gacgaactcc gaacccgtgg ccgtcgtcgg
     1321 caggggcacc ggcatcgacg acgccggctg ggcgcgcaag acggcagcga ttcgcgacgc
     1381 gctgtaccgg gcccgcggat tgtccgcgga tccggttggg ctgctgcggg tttgcggcgg
     1441 agcggacttc gccgcaatgg cgggcttctg tgcccaggcc gctgtgcgcc gcacgcccgt
     1501 gctgctcgac ggcgtggtgg tgaccgcggc cgcgttggtg gccgaccgac tcgcgccagg
     1561 cgcccggcag tggtggcagg cgggacaccg gtcaaccgag ccggcacatg cgcttgcgct
     1621 gcagcagctc gacctcgaac cgatcatcga cctgcggatg cggctcggcg agggcaccgg
     1681 cgccgcggtc gccttgcccg tcgttcgcgc cgccgttgcc gcgctgtcgt cgatggccac
     1741 tttcgacgag gcaggcgtca ccgcgtgatc cgctcggtca caggcgcatt ggcgtttgcg
     1801 accgttctgc ccgtgcgcgg ctcggccggg gtgggccgcg gggtcatcac cgccttgccg
     1861 gttgtcggcg tcgcgctcgg ggcggtggcg ggcggggtgg tgtgggcgtc gtcgtgggcg
     1921 ttcggcgcag gcagtccgct tggcggcgcg ctggcggtca cggtattgct gatcgcaacg
     1981 cgcggcctgc acatcgacgg gttgtcggat accgccgatg cgctcggctg ctacgggccg
     2041 ccggaccgag cgctgcaggt gatgcgcgac ggctcggcgg gaccgttcgg tgtcgccgcg
     2101 gtggtggtgg cgattctggt gcaggtcctg gcgttcgcgc agctgagtat cgtcgccgtg
     2161 gtcgtcgccg tgacggccgg cagggtggcc gcggtggcgg cgtgccggcg cgcggtgccc
     2221 gccgcgcagg gcagctcgct gggtgcacgt gccgcaggca cccagccggc cgcggtggtg
     2281 ttgctctgga tcatcggggt ggcggtgctg gccgtcctgg cagatgcgcg acgctggcag
     2341 ggcccgctgg ccgttctggt cgcgctcgtc tgcgctgggg cactggtcgc gcactgcgtc
     2401 cggcggttcg gcgggatcac cggcgacgtg ctgggcgcgg cgatcgaggt gacgacgacg
     2461 gtgaccgccg tcgcgctggc gatccgttag ttcggggcca ccggtttgtc atgtcaaaga
     2521 acactgattt gtatcagaaa cagttacaaa cagacagaaa ttgacacgga aagggtttaa
     2581 gttctagtgt cattttctgt cggtttgtaa ctagattaga tacaaagtag gtggatgtga
     2641 cacgcaccat ccctccatcg ctagcgccca tcctggaaca gctcgagctg aacggcgacg
     2701 aactggtcac gacggagcgc ctcgacgagt tggtacgcgc gactggtatc gacacaccga
     2761 cacgagttgt tgcggcccga ctgcgggacc gtggatggct gctgaccaca ggtcaacgcg
     2821 gagtatggga attcgcacct gcggccgtcg cgggaccctt tagccggggc ggacccaccc
     2881 gcctattgcg ttcgttgttg gctcggcgga acgtcacatg cggacttacg ttccaggcag
     2941 cagcttgggc cattggagct gcggaccgag ctccaatgcg aatcgaggtc gcagttgcta
     3001 acgaacgtga tgttcggtgg atgccggacg ggttagacgt ctcgatcttc gatccgcagc
     3061 tgccgtatcg cacggcaaag ggagtaccga ctctgcagcc tgcgagcgtt ctaacgcata
     3121 tggcgacgac ccccgcccgc gttcggtctt ggtcaagtgc tgccgaatgg cttcccgata
     3181 tcgccgctga gatcacaatc gccgacgcac tcacagaact tggaggacgt cccgccactg
     3241 taggtgcgcg gctcggctac ctgctacagg gattgcggcc tgatgtcgct gagcagatca
     3301 ccagaccttc gacgaagacg tggttcggtc cgcgacgcaa gcttgtccgc aatgacgagc
     3361 gctggaaaat cgctgacacg atactcccgt tcgacccccg tgacttcgag ccggttacgt
     3421 gacctcgcaa cagatctcga tcacggcagg ctggattgcg cgacacacac cgaccggtgc
     3481 tggcgctggc ggccgcgacg ccgccgtcat cgatatcgcg caggaccttc tgcttcgtga
     3541 cttgcatacg cgcgacgcac tggaggctat tgttttcaag ggcgggacag ccctgcgcaa
     3601 gttgtacgca ggaaaccaag gacgattctc ggtcgatctc gacttcagtc tcgcccgagc
     3661 gaacggcgcc tacagcgcgt gacatgtgcg acttggcatg gatcgctcag catcaacacg
     3721 acgttggcag ccttgatctc aacctcatcc gtcgacttgc tgtactcaag atatgggtcg
     3781 attccaacgg actggcagga ggaaaggccc actggagaca aggacacaaa ccgtgtgagt
     3841 tcgaccccac ctcttggtta
//
LOCUS       DASRJA010000754         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4083465,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000754 DASRJA010000000
VERSION     DASRJA010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4083465"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VFC01_34060"
     CDS             <1..775
                     /locus_tag="VFC01_34060"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZN84685.1"
                     /translation="VYVATPVDVHAEQTIAAAEAGKHVLCEKPMAMTTAECDRMIAAC
                     RAHHVRLGIAYYRHFYPVILRVKAMLESGDIGQPVFAQVNAFEYFDPAPDHPRRWLLD
                     PARAGGGPMMDFGCHRLEVLMHLFGAVRRTAAVTANVVFDRAVEDTAAVLLQFEGGPC
                     AAVTVTHAAEERQDTLQVFGTRGSVHVDELNAGSLRIRTGNQSRVESHPPAANIHRPL
                     IDDFVEALLTDREPAVTGEIGRAVAAIEDEIYAVAPSGR"
     gene            921..1859
                     /locus_tag="VFC01_34065"
     CDS             921..1859
                     /locus_tag="VFC01_34065"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZN84686.1"
                     /translation="MLSVDPVFGNLVQANSAIKRLWTGALWAEGPAWSGQGRYLVWSD
                     IPNNRQLRWLDDDEHVTVFRSPSNNSNGNTFDFAGRQLSCEHLTRRVVRYEHDGSTTV
                     IAESYNGKRLNSPNDVAPHPDGSYWFTDPPYGRSLYEGRPEKRELPTAVYRADGKGGL
                     TQVVAEEQVPDPNGIAFSPDYKKLYVVSTGRGPGDAGPGGKGNMFVFDVTADNRTANG
                     RVFSEFMIDGVRCSPDGVRCDVDGNVWCSSNAGRAVGYSGVTVWTPQGKLIGRIRLPE
                     VCGNLCFGGAKRNRLFMAASQSLYAVYVNTQGAGPG"
BASE COUNT          359 a          690 c          732 g          339 t
ORIGIN      
        1 cgtttacgtc gcgacgccgg tggacgtcca tgcggagcag acgatcgcgg ccgccgaagc
       61 cggcaagcac gtcctgtgcg agaagccgat ggcgatgacc acggcggaat gcgatcgcat
      121 gatcgcggcc tgccgcgcgc atcacgtccg gctgggcatc gcctattacc gtcatttcta
      181 tcccgtgatt ctgcgcgtca aagccatgct ggagtcgggc gacatcgggc aaccggtctt
      241 cgcgcaggtg aacgcattcg agtattttga tcccgcaccg gaccacccgc gccggtggct
      301 gctggatccg gcccgggccg gcggcgggcc gatgatggac ttcggctgcc accgcctgga
      361 ggtgctgatg catctgttcg gcgccgtgcg ccggacggcc gccgtcaccg ccaacgtggt
      421 gttcgatcgc gccgtcgagg acacggccgc ggtgctgctg cagttcgagg gcgggccgtg
      481 tgcggccgtg acggtcacgc acgcggccga agaacggcag gacacgctgc aggtgtttgg
      541 cacgcgcggc tcggtgcacg ttgacgagtt gaacgcgggc tcgctccgga tccgcacggg
      601 caatcaatcg cgggtagaat cgcatccgcc cgcggcaaac atccatcggc cgctcatcga
      661 cgacttcgtc gaggcgctgc tgacggaccg ggaaccggcg gtgaccggcg aaatcgggcg
      721 agccgtcgcc gccatcgagg acgagattta tgctgtcgcg ccgtcaggcc gttaaggctc
      781 ttggcatcct gggcggagcg gtcgtcaccg ggcgggcggg ctcggcgcag acgccgcagg
      841 gaccgccgag cactgtcacg aatcccccca gggacttcgg tcccaacgcc ccgcccacca
      901 cctacttcac cgatcccgac gtgctgagcg tcgatccggt gttcggcaac ctggtgcagg
      961 caaactcggc aatcaagcga ctgtggaccg gcgcgctgtg ggccgagggg cccgcatgga
     1021 gcggccaggg gcggtatctg gtctggagcg acatcccgaa caaccgtcag ctgcgctggc
     1081 tcgacgacga tgagcatgtg accgtgttcc gcagcccgtc caacaactcc aacggcaaca
     1141 ccttcgattt cgccggccgt cagctctcgt gcgagcacct cacgcgccgc gtcgtccgct
     1201 acgaacacga cggatcgaca acggtgatcg cggagtcgta caacggcaag cggctcaact
     1261 cgccgaacga cgtggccccg cacccggatg gcagctactg gttcaccgat ccgccgtacg
     1321 gccggagttt gtacgaggga cgccccgaaa agcgcgagct cccgacggct gtctatcgcg
     1381 cggacggcaa aggcggcctg acgcaggtgg tggcggaaga gcaggtgccc gatcccaacg
     1441 gcattgcgtt ttcgccggac tacaagaagc tgtatgtcgt cagcaccggg cgcggtccgg
     1501 gcgatgccgg gccggggggc aagggcaata tgttcgtctt cgatgtgacc gccgacaacc
     1561 ggaccgcgaa cgggcgcgtc ttttcggagt tcatgatcga cggcgtcagg tgttcgcccg
     1621 acggcgtgcg ctgcgacgtg gacgggaacg tctggtgctc gagcaacgcc ggccgcgccg
     1681 tcggctatag cggagtgacc gtctggacgc cgcagggcaa actgatcggc cgcatccgcc
     1741 ttccggaggt gtgcggcaat ctctgtttcg gaggcgcgaa gcggaatcgg ctgttcatgg
     1801 ccgcgagcca gtctttatac gcggtttatg tgaacacgca gggtgcgggt cccggataac
     1861 gcgctcgtcc gggcgctgag cgtgcggacg agaatctgcc acagagacac agaggcaccg
     1921 agccgccgat tcgacggcgt gggcctgcgg agcaggccca ccgagcggcc gcaaacggtt
     1981 cacggaaacg agccgcattg gtgacggtcg gcggttcgtt tccgcgaacc gttttcggcc
     2041 gaccgcgccg cttcgcggcg cgccgtcgaa tcgatcgtct tggcctggca cgctcagcgc
     2101 ctgagagaac cgaggtccat
//
LOCUS       DASRJA010000755         6241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_534913,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000755 DASRJA010000000
VERSION     DASRJA010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534913"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1137)
                     /locus_tag="VFC01_34070"
     CDS             complement(31..1137)
                     /locus_tag="VFC01_34070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HZN84687.1"
                     /translation="MTETAAIASRAQPRSWVDNAVRLIEADARRSADTHLLRYPLPAA
                     WCDGCNVELYLKDETTHITGSLKHRLARSLFLYALCNGWIGEGTTVIEASSGSTAVSE
                     AYFAALLGLPFIAVMPSSTSAAKIALIESQGGRCHFVAQSSQVYAEAERLAEETGGHY
                     LDQFTNAERATDWRGNNNIAESIFEQLRDEAHPIPEWIVVGAGTGGTSATIGRFIRYR
                     RHATRLCVVDPENSAFFPSYAQGRRDVVTGMSSRIEGIGRPRVEPSFLPDVVDRMMVV
                     PDAASIAAAHHVSAVLGRRVGPSTGTNLWGAFGLLAEMIAEGRSGSVVTLLADSGDRY
                     ADTYFCDEWLTSQGLDPSDSASVLVEFERSGAWP"
     gene            complement(1134..2093)
                     /locus_tag="VFC01_34075"
     CDS             complement(1134..2093)
                     /locus_tag="VFC01_34075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZN84688.1"
                     /translation="MPAPSTVLKSTAAVTLGSVAAGIGYASLIERNAFVVREVTMPML
                     SPGSSPLKVLHISDIHMRPKQRRKQAWLRELARLEPDLVVNTGDNLAHPKAVPAVVQS
                     LSDLLGVPGVFVFGSNDYFGPRLKNPANYLTNRGHRVHGEPLPWQDLRAAFTERGWLD
                     MTHTKRELEVGGLRIALAGVDDPHVSRDRYDTIAGPASPAADLTLGLTHSPEPRVLDR
                     FAADGYQLVMAGHTHGGQLCLPFYGAIVTNCDLDRSRAKGASRWGARTQLHVSAGIGT
                     SPFYPVRFCCRPEATLLTLVAAPIGGRDAGMRAGQSHPSVSVR"
     gene            complement(2178..4649)
                     /gene="ponA2"
                     /locus_tag="VFC01_34080"
     CDS             complement(2178..4649)
                     /gene="ponA2"
                     /locus_tag="VFC01_34080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase/D,D-transpeptidase PonA2"
                     /protein_id="HZN84689.1"
                     /translation="MPESPPPRPSPPPPKTVTVIKLAWCCLLASVVVAALMFPVVGGF
                     GLLSNRASDVVANGSAQLVEGEVPQVSTMVDAKGNVIAWLYSQRRFEVPSDQIANTMK
                     LAIVSIEDKRFAEHNGVDWQGTLTGLSGYLSGNLDTRGGSTLEQQYVKNYQLLVIAQT
                     DAEKRAAIETTPARKLREIRMALTLDKTFAKPEILTRYLNLVSFGNGAYGIQDAAQTY
                     FGINASELNWQQAALLAGMVQSTSTLNPYTNPDGALARRNLVLDTMVDNLPEHADELR
                     AAKQEPLGILPQPNELPRGCIAAGDRAFFCDYVLEYLARAGISKEQVAKGGYLIKTTL
                     DPDVQIPVKSAIDGIASPDLDGVASVMSVIRPGKDSHPVVAMASNRTYGLNTDAGETM
                     QPQPFSLVGDGAGSTFKLFTTAAALEMGMGINAQLDVPGQFQAKGLGSSDTPGCPKET
                     WCVKNAGGYRGQMNVTDALATSPNTAFAKLISQVGVQRTVDMAVRLGMRSYALPGTAR
                     DYDPDSNESLADFIKRQNLGSFTLGPFELNPLEFSNVAATLASGGVWCPPNPIDKVID
                     RHGKDVAVTTETCEQVVPEGLANTLANAMSKDDQGAGTAAGSAGSVGWDLPMSGKTGT
                     TEAHRSSAFLGFTNTLAAGSYIYDDSTTPGDLCSFPLRQCGSGNLFGGNEPARTWFTA
                     MKPIANNFGPVALPPTDPRYVEGAPGSRVPSVAGLTQDTARQRLREAGFQVADQASPV
                     NSTAPYGAVVGTSPTGQTVPGSIITIQISNGIPPPPPPPPPGAIPGLPGMPPEIGQTV
                     VEIPGLPPITVPVLGPPPPPAPPPP"
     gene            4943..5299
                     /locus_tag="VFC01_34085"
     CDS             4943..5299
                     /locus_tag="VFC01_34085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HZN84690.1"
                     /translation="MSATRPAARRTSPNQSAHSFVQGAEAEARIAWVSQARCRQADPD
                     ELFVRGAAQRKAAVICRHCPVILECGADALDNRVEFGVWGGMTERQRRALLKQHPEVV
                     SWADFFAAQRKHRSAV"
     gene            complement(5315..>6241)
                     /locus_tag="VFC01_34090"
     CDS             complement(5315..>6241)
                     /locus_tag="VFC01_34090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HZN84691.1"
                     /translation="DLGNTPQRVPLAPEVPGELHAMMLDMRRTFDEMVIQYSGRERGE
                     AILDNQFYQTVATSLAGTQEYMAMEKLGQLLAQDKWDLIVVDTPPSRNALDFLDAPQR
                     LGSFMDSRLWRLLLAPGRGIGRLVTGAVGLAMKALSTVLGSQMLSDAAAFVQMLDTTF
                     GGFREKADRTYELLKRRGTQFVVVSAAEPDALREASFFVDRLSEEHMPLAGLILNRTH
                     PTLSDLHAEKAEEAAEDLAEKDPDGLAAAVLRIHAERAMTAKREIRLLSRFTGANPHV
                     AIVGVPSLPFDVSDLEALRAIADQITGEASAA"
BASE COUNT         1008 a         2110 c         2060 g         1063 t
ORIGIN      
        1 cgacggaccg ctaatcggtc ggtccgacga tcagggccag gccccggatc gctcgaattc
       61 gaccaagact gacgcggagt ccgaggggtc cagcccctgg ctggtcagcc actcgtcaca
      121 gaagtaggtg tccgcgtagc ggtcaccgct gtcggcgagc agggtcacga ccgacccgct
      181 gcgtccctcc gcgatcatct cggccagcag gccgaaggca ccccacaaat tcgtccccgt
      241 tgacggcccc acccgccggc ccagcaccgc gctgacgtga tgcgctgccg cgatcgacgc
      301 cgcatccggc accaccatca tgcggtccac cacgtctggc aggaatgacg gctccacccg
      361 tgggcggccg atgccctcga tccgagacga catcccggtc acgacgtcgc ggcggccctg
      421 agcgtaggac gggaaaaacg ccgagttctc cgggtcgacg acgcacagcc gggtggcgtg
      481 gcgccgatac cggatgaacc gtccgatcgt tgcgctggtg ccgccggtac ccgcaccgac
      541 cacgatccac tccggaatcg gatgcgcctc gtcacgcaac tgttcgaaga tcgactcggc
      601 gatgttgttg tttccgcgcc agtcggtcgc gcgctcggcg ttggtgaact gatccaagta
      661 gtggccgccc gtctcttcgg ccagccgctc cgcctccgcg tacacctgag atgactgggc
      721 cacgaaatgg caacggccgc cttgagattc gatcagggcg atctttgcgg cactggtaga
      781 cgacggcatc actgcgatga atggcaagcc cagtagcgcc gcgaagtagg cctccgacac
      841 ggccgtcgac cccgacgacg cctcgatgac agtggtgccc tcaccgatcc acccgttgca
      901 gagcgcatac aggaacaacg accgcgccag ccgatgcttg aggctgccgg tgatatgcgt
      961 ggtctcgtcc ttgaggtaga gctcgacgtt gcacccgtcg caccaggccg caggcagcgg
     1021 ataccgaagc agatgggtgt cggcgctgcg ccgggcgtcg gcctcgatca accggactgc
     1081 gttgtccacc cacgaccgtg gctgggcgcg actggctatt gcggccgtct cggtcaccga
     1141 accgagacgc tcgggtggga ctggcccgcc cgcatcccgg cgtcgcgacc cccgatgggc
     1201 gccgccacca gcgtcagcag cgtggcctcc ggcctgcagc agaaccgcac gggatagaac
     1261 ggcgacgtgc cgatgccggc ggagacgtgc aactgtgtac gcgcgcccca gcgtgacgcg
     1321 cccttggccc gcgaccggtc caggtcgcaa ttggtgacga tcgcgccgta gaacggcagg
     1381 cagagctggc cgccgtgcgt gtggccggcc atcaccagct ggtatccgtc ggccgcaaag
     1441 cgatccagca cccgcggttc gggcgaatgc gtcagaccca gcgtcaggtc cgctgccggg
     1501 ctcgcaggcc ccgcgatggt gtcgtaacgg tcccgggaca cgtgcgggtc gtcgaccccg
     1561 gccagcgcga tccgcagccc acccacctcg agctcgcgct tggtgtgcgt catgtcgagc
     1621 cagccgcgct cggtgaacgc cgcccgcagg tcctgccacg gcagcggctc gccatggacc
     1681 cgatgcccgc ggttggtcag gtaattcgcc gggttcttca gccgcgggcc gaagtagtcg
     1741 ttgctgccga acacgaacac ccccggcacg cccagcaggt cgctcagtga ctggacgacg
     1801 gcgggcactg ccttcggatg ggccaggttg tcgcccgtgt tgacgacgag gtcgggctcc
     1861 aagcgggcga gctcacgcag ccaggcctgc ttgcgccgct gcttgggccg catgtggata
     1921 tcgctgatgt gcagcacctt cagcggcgac gaccccggcg acagcatggg catggtcacc
     1981 tcgcgcacca cgaacgcatt gcgttcgatc agcgacgcgt aaccgatccc tgctgcgacc
     2041 gaaccaaggg tcacggcggc ggtgctcttc aggaccgtcg aaggggcagg catgcaaaga
     2101 gactactgcc actgtgccgc aggcaccgac tctcggcggc gcgccgtggc caatcgcaca
     2161 gcgtgcttac ggtccgatca cggcggtggc ggcgctgggg gtggaggcgg accgagcacg
     2221 ggcacggtga tcggcggtag ccccggtatc tcgacgaccg tttggccgat ctcgggcggc
     2281 atccctggca gccctgggat cgcgcccggt ggcggcggag gcggtggcgg cgggatgccg
     2341 ttggagatct ggatcgtgat gatcgagccc ggcaccgtct gcccggtcgg ggacgtcccg
     2401 acgacggcgc cgtacggcgc ggtgctgttg accgggctgg cctgatcggc gacctggaaa
     2461 ccggcctccc ggagccgctg acgggcggtg tcctgcgtca ggccggccac gctgggcacc
     2521 ctggaccccg gcgcgccctc cacatagcgc ggatcggtcg gcggcagcgc cacggggccg
     2581 aagttgttcg ctatcggttt catcgcggtg aaccaggtgc gggcgggttc gttaccaccg
     2641 aagaggtttc cggagccgca ctggcgcagc gggaacgagc agaggtcgcc cggagtcgtc
     2701 gagtcgtcgt agatgtagct gcccgcggcc agcgtgttcg tgaacccgag gaacgccgac
     2761 gagcggtgcg cctccgtcgt gccggtcttg cccgacatcg gaaggtccca gcccaccgag
     2821 ccggccgacc cggctgcggt acctgcgccc tgatcgtcct tgctcatcgc gttggccaag
     2881 gtgttggcga gcccctcggg caccacctgt tcgcatgttt cggtggtgac ggccacgtcc
     2941 ttgccgtgcc tgtcgatgac cttgtcgatc gggttgggcg ggcaccacac gcctccggag
     3001 gccagcgtgg cagccacatt ggagaactcc agggggttca gctcgaacgg gcccagcgtg
     3061 aacgatccca ggttctgccg cttgatgaaa tcagccaggc tctcgttgct gtcggggtcg
     3121 tagtcgcggg ccgtgcccgg cagtgcgtag gaccgcatgc ccagccttac ggccatgtcg
     3181 accgtgcgct ggacaccgac ctgcgagatc agcttggcga aggcggtatt cggcgacgtg
     3241 gccagtgcgt cggtgacgtt catctgtccg cggtagccgc ctgcgttctt cacacaccag
     3301 gtctccttcg ggcaacccgg ggtgtcgctg ctgccaaggc ccttggcctg gaactggccg
     3361 ggtacgtcca gctgggcgtt gatgcccatg cccatctcca acgcggccgc ggtggtgaag
     3421 agcttgaatg tggaccctgc accgtcgccg acgagggaga acggctgcgg ctgcatcgtc
     3481 tcgcccgcgt cggtgttcag gccgtacgtg cggttgctgg ccattgcgac gaccggatgg
     3541 ctgtccttac cgggcctgat cacgctcatc acgctggcga cgccgtcgag gtcggggctc
     3601 gcgatgccgt cgatcgccga cttcaccggg atctgtacgt cgggatcgag cgtggtcttg
     3661 atcaagtagc cgcccttggc gacctgctcc ttgctgatgc cggccctggc gaggtactcg
     3721 agcacgtagt cgcagaagaa tgcgcgatcc ccggctgcga tgcagccacg cggcagttcg
     3781 ttgggctgcg gcaggatgcc caacggctct tgcttggctg cccgcagctc atcggcgtgc
     3841 tccggcaagt tgtcgaccat ggtgtccagc accaggtttc gacgtgccag cgcaccgtcg
     3901 gggttggtgt acgggttcag cgtgcttgtc gactggacca tgccggccag cagcgccgcc
     3961 tgctgccagt tcaattcaga ggcgttgatc ccgaaatacg tctgcgccgc gtcctggatg
     4021 ccgtacgcgc cgttgccgaa cgagaccaga ttgaggtaac gggtcaagat ctccggcttg
     4081 gcgaatgtct tgtcgagcgt cagcgccatt cggatctcgc gcagctttcg cgcgggcgtc
     4141 gtctcgatgg ccgcccgctt ctcggcgtcg gtctgcgcga tcaccagcag ctggtagttc
     4201 ttcacgtact gctgttccag cgtcgagccg ccgcgggtgt ccaggtttcc agacagatac
     4261 ccggacaggc cggtgagcgt gccctgccag tccacgccgt tgtgctcggc aaaccgcttg
     4321 tcctcgatcg acacgatcgc cagcttcatc gtgttggcga tctggtcgct gggcacctcg
     4381 aaccgtcgct gtgaatacag ccatgcgatg acgttgccct tggcgtcgac catcgtcgac
     4441 acctgcggca cctctccctc gaccaattgg gccgaaccat tggcaaccac gtcggaggcg
     4501 cggttggaca gcaacccgaa cccgccgacc acgggaaaca tcagcgccgc gacgaccacg
     4561 ctggccaaca aacagcacca ggccagcttg atgaccgtga ccgttttcgg gggcggaggc
     4621 gacggtcggg gcggggggct ctccggcatg cgttacagag taacgacgtc gcaaaatggc
     4681 agcttctgac ggtcgattgc ccggttcgct ggacgtccag atcagctgtt gcgtcaattt
     4741 ttggtcctga cggcacggtc gtcccaaaaa agtagggatt cacctattgc gcagaacggc
     4801 cctgaccacc tacgttgggc agacagtgcg atgtaggtca cacccgaccc cagcactgtg
     4861 gcgtagatcg catcagcgct ctacaccgga ggactacgcc gtcgcggagc tagccgggcg
     4921 gcctggaacg aagggatcgc tggtgtcagc tacgaggccc gccgctcgca ggacaagccc
     4981 gaatcagtca gctcacagtt ttgtccaagg cgcagaggcc gaggctcgta tcgcctgggt
     5041 atcccaggct cgatgccgcc aagccgatcc agacgaactt ttcgtccgcg gcgcggccca
     5101 gcgcaaagcc gccgtcattt gccggcactg tccggtgatt ctcgagtgtg gtgccgatgc
     5161 cctggacaac cgcgtcgagt tcggcgtttg gggcggcatg accgaacgtc agcgccgcgc
     5221 gctgctcaag cagcaccccg aggtggtttc ctgggccgat ttcttcgccg cccagcgcaa
     5281 acaccgcagc gcggtctaga tcgcctctac gccgctatgc ggcggaagcc tcgccggtga
     5341 tctgatcggc gatcgccctc agcgcctcca gatccgacac gtcgaacggc agcgacggca
     5401 cgcccacgat cgccacgtgc gggttggcgc cggtgaaccg cgacagcagc cggatctccc
     5461 gcttcgccgt cattgcccgc tcggcgtgaa tgcgcagcac cgccgcggcc aacccatcgg
     5521 gatccttctc cgccaggtcc tcggcggctt cctcggcctt ctcggcgtgc aggtcgctca
     5581 gtgtcgggtg agtgcggttg agaatcaacc cggccaacgg catgtgctct tcggagagcc
     5641 ggtcgacgaa gaaggacgcc tcccgtagcg cgtccggctc ggcggccgac accacgacga
     5701 actgtgtgcc gcgccgcttg agaagctcgt acgtgcggtc ggccttctcc cggaacccgc
     5761 cgaatgtggt atccagcatt tgaacgaaag cggctgcgtc ggaaagcatt tgcgaaccca
     5821 gcaccgtcga cagcgccttc atcgccaagc ccaccgcgcc ggtcaccagc ctgccgatgc
     5881 cgcgaccggg cgccagcagc agccgccaca gccgactgtc catgaagctc cccagccgct
     5941 gcggcgcatc caggaagtcc agcgcgttgc gtgacggtgg ggtgtcgacg acgatcagat
     6001 cccacttgtc ttgggcgaga agctgaccca gcttctccat cgccatgtat tcctgcgtgc
     6061 ccgcgagcga cgtggccacg gtctgataga actggttgtc cagaatcgct tcgccccgtt
     6121 cgcgtcccga gtactggatc accatttcgt cgaaggtgcg gcgcatgtcg agcatcatcg
     6181 cgtgcagttc gcccggcacc tccggggcga ggggaacccg ttgcggagtg ttgccgagat
     6241 c
//
LOCUS       DASRJA010000756         4289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2892302,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000756 DASRJA010000000
VERSION     DASRJA010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2892302"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VFC01_34095"
     CDS             <1..865
                     /locus_tag="VFC01_34095"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZN84692.1"
                     /translation="AGLRGGEFSSVRQPDGYVAHTTFQHIAEAIGVDNIVDGTAIVGR
                     ARYVKGDEEISRLAATVRVGEQSLEAMAAAADVGVSQAEVFGQMLLAQVRAGADELHV
                     AWCPGRWGAPRHRYVTTPPGVLTPGLYVSTELMPEIRGYQAQVAQPMVIGEPDARALE
                     VFDRNVAAFDAGLAAMRPGRRWGDVFDAVAAAIGDVDGEMFTLLHGRGLGNDGPLVIP
                     DGAPRESGIAAEPIMANTTFILKPFLKVSEAPAAFARTHDVTWGDTVVVTETGGQRLG
                     TRRRELTVVGA"
     gene            888..1360
                     /locus_tag="VFC01_34100"
                     /pseudo
     CDS             888..1360
                     /locus_tag="VFC01_34100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216491.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
     gene            1392..1634
                     /locus_tag="VFC01_34105"
     CDS             1392..1634
                     /locus_tag="VFC01_34105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84693.1"
                     /translation="MNDMEKSVAARLRRESELAGDAVRGTEDEIMELGVHCAYAVGFE
                     RLAATCDVTDDVPEAFRSKSEMPVAPWVAEGVAASG"
     gene            complement(1631..1822)
                     /locus_tag="VFC01_34110"
     CDS             complement(1631..1822)
                     /locus_tag="VFC01_34110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HZN84694.1"
                     /translation="MGRAATIARQIGWYWGTLMGDNHYRRYVEHRRSTHPGEPVMTER
                     EYWRMRHDAIESNPNPRCC"
     gene            complement(1768..4062)
                     /locus_tag="VFC01_34115"
     CDS             complement(1768..4062)
                     /locus_tag="VFC01_34115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HZN84695.1"
                     /translation="MATPTAAPSDRIEERDGDVTYIRTDKDLPPVAIIDRSPITTRHK
                     IIFGIIAVLGAVAWAIIAFFRGETVNAVWFVIAAICTYVIGFRFYARLIEMKIVRPRD
                     DNATPAEVFENDTDYMPTDRRVLFGHHFAAIAGAGPLVGPVLAMQMGYLPGTIWIIIG
                     AVFSGCVQDYLVLWISTRRRGRSLGQMVRDELGSVGGAAAIIGIITIMTILLAVLALV
                     VVQALAQSPWGVFSIAMTIPIAIFMGIYLRFLRPGRVSEVSLIGVALLLLAVISGGWV
                     AETAWGESWFGLSAVTIGWCLIVYGFAASVLPVWLLLAPRDYLSTFMKVGTIALLAIG
                     VLIARPVMQAPAISDFAGSGQGPVFAGALFPFLFITIACGALSGFHSLIASGTTPKML
                     EKESQMRLIGYGGMITESFVGIMALITAAILNQHLYFTLNAPAASTGGTAATAAEYVN
                     KLPLTGDPITAAQIEQAAADVGEKSIVSRTGGAPTLAIGMSEVLHRVFGGTGLKAFWY
                     HFAIMFEALFILTTVDAGTRVARFMLSDGLSNLGGPLRKLKDPSWRVGAWTCSLAVVA
                     LWGSILLMGITDPLGGINSLFPLFGIANQLLAAIALTIVSVVVVKKGYLKWLWIPGIP
                     LLWDLTVTLTASWQKIFSGDPKVGFWTQHFQYRNALDAGKTTFGQAKNVDQMEAVIRN
                     TFIQGTLSIVFALMVVIVFGTGLIMVMRVLRGGGTPLTEDEPVASKLFAPSGLIPTKA
                     EREVQKQWDALPQSHARSVGTGAH"
BASE COUNT          807 a         1341 c         1396 g          745 t
ORIGIN      
        1 cgctggactt cgcggtggtg aattctcgtc tgtccgccag cctgacggct atgttgccca
       61 caccaccttt caacacatcg ctgaggcaat aggtgtcgac aacatcgtcg acggcacagc
      121 gattgtcggc agagcgcgat acgtcaaagg tgacgaggag atcagccgtc tcgccgcgac
      181 tgtccgagtc ggcgaacaat ctctggaggc catggccgcc gctgcggatg tcggggtgtc
      241 ccaagcggaa gtcttcggtc agatgttgtt ggcccaagtg cgggccggcg ccgatgaact
      301 gcatgttgcc tggtgtccag ggcgctgggg agcgccgcga caccgatatg tcaccacccc
      361 gcccggtgtg ctgacgccgg gactttatgt gtcgactgag ttgatgcctg agatccgcgg
      421 ctaccaggcg caggtcgctc agccgatggt gattggagaa cccgacgcgc gagccttgga
      481 ggtcttcgat cgaaatgtgg ccgccttcga cgccggctta gccgcgatgc gaccgggccg
      541 gcgatggggc gacgtctttg acgcggtggc ggcggccatc ggggatgtcg acggggagat
      601 gttcacactg ctacacggac gtggtctggg caatgacggg cccctcgtga tacctgacgg
      661 cgcgccgcgc gaaagcggta ttgccgcgga accgattatg gcgaacacga cattcatctt
      721 gaagccgttc ctgaaggtgt ctgaggctcc ggccgcgttt gcgcgcaccc atgacgtcac
      781 ctggggcgac accgtcgtgg tcaccgagac gggcggtcag cgcctgggta cgcgacgccg
      841 cgaactcacc gttgtcgggg cctgagcagc acatgctcgc gggcggcttg cgcggaaagt
      901 cggcagcgat acttgcgctg tcagtcgggg tcctggacgt cgtcactgcg ccgaatgccc
      961 tagccgacaa caagcggctc aacgacgggg tggtcgccaa cgtctacacc gtccagcatc
     1021 aggccggctg caccaacaac gtcacgatca gcccgcagtt gtagctggcc gcccagtggc
     1081 atgccaacga cgtgctcaac aaccgcgcac tcgacgccga catcggctcg gacgggtcga
     1141 cggtgcagga ccgcgccaac gccgccgggt ttaagggtgc cgtcgccgag acggtcgcga
     1201 tcaaccggca ctggcgatca acggcgtcga gatcatcaac cagtggtact accgcccgga
     1261 ctacatggcg atcatgtcga actgcgccaa ctcgaaaatg ggcgtgtggt cggtgaccag
     1321 cctggaccgc agcgtcgtcg tcgccgtcta cggccaaccg acctgaatcg gtcggtccaa
     1381 aaggttggtg agtgaacgac atggaaaaga gtgtggcggc caggctacgc cgcgagtcag
     1441 agctggcggg cgatgccgtg cgcggcaccg aagacgagat aatggagctc ggcgtgcact
     1501 gcgcctatgc ggtgggcttc gagcgcctgg cggcgacgtg cgacgtcacc gacgatgtgc
     1561 ctgaggcgtt tcgatcgaag tcggaaatgc ctgtggcgcc gtgggttgca gagggcgtcg
     1621 ccgcctccgg ctagcagcag cgcggattgg gattcgactc gatcgcgtcg tgccgcatcc
     1681 gccagtactc gcgctcggtc atgaccggct cgcctgggtg ggtgctcctg cgatgctcga
     1741 cgtagcgccg gtagtggtta tcgcccatca atgtgcccca gtaccaaccg atctggcgtg
     1801 cgattgtggc agcgcgtccc attgcttttg cacctcccgt tccgccttgg tcggaataag
     1861 gcctgatggc gcgaagagtt tcgacgcgac cggctcgtct tctgtcaacg gggtgccgcc
     1921 gccccgcagc accctcatga ccatgatcag ccctgtgccg aacacgatga ccaccatcaa
     1981 cgcgaacacg atcgacagcg tgccctggat gaacgtgttg cggatgacgg cttccatttg
     2041 gtcgacgttt ttcgcctgac cgaacgtcgt cttaccggca tccaatgcat tgcggtactg
     2101 gaagtgttgc gtccaaaatc cgaccttcgg gtcgccggag aagatctttt gccacgacgc
     2161 ggtcagcgtg accgtcagat cccacaacaa tggaatgccc ggaatccaca accatttcag
     2221 gtagcccttc ttcacgacga caaccgacac gatggtcagc gcgatcgcgg cgagcagctg
     2281 gttggcgatg ccgaacagcg ggaacaggga gttgatcccg ccgagcggat cggtgatgcc
     2341 catcaacagg atgctgcccc acagcgcgac gacggccagg ctgcacgtcc acgcgcccac
     2401 ccgccagctc ggatccttga gcttgcgcag cggcccgccg agattggata gcccgtcgga
     2461 cagcatgaag cgggccaccc gggtgccagc atcgaccgtc gtgaggatga acaacgcctc
     2521 gaacatgatc gcgaagtggt accagaacgc cttcaggccg gttccgccga acacgcggtg
     2581 gagcacttcc gacatgccga tcgcgagcgt cggtgcgccg cccgtgcgcg acacgatcga
     2641 cttctcgccg acgtcggcag cggcctgctc gatttgtgca gcggtgatcg ggtcaccggt
     2701 gagggggagt ttgttgacgt attcggccgc cgtggccgcg gtgcctcccg tagatgccgc
     2761 gggtgcgttg agcgtgaagt acaaatgctg gttgaggatc gcggcggtga tcagcgccat
     2821 gatgccgacg aaggattcgg tgatcatgcc gccgtagccg atcagccgca tctggctttc
     2881 cttctccagc attttcggcg tggtgccgga ggcgatgagc gagtggaagc cggacagcgc
     2941 gccgcacgcg atcgtgatga acaggaacgg gaacagtgcg cccgcgaaca ccgggccctg
     3001 gccgcttccg gcgaagtcgg agatggcggg tgcctgcatg acggggcggg cgatcagcac
     3061 gccgatcgcg agcagggcga tggtgccgac cttcatgaac gtcgacaggt agtcgcgcgg
     3121 cgccagcagc aaccacaccg gcagcaccga agcagcgaag ccgtagacga tcaggcacca
     3181 cccgattgtc accgcagata ggccgaacca cgattcaccc caagcggttt cggcgaccca
     3241 tccgccggag atgacagcca gtagcaaaag cgcgacgccg atcaacgaca cctcggacac
     3301 ccgcccggga cgcaggaatc gcaaatagat ccccatgaag atcgcgatgg ggatcgtcat
     3361 cgcgatcgag aagacgcccc acgggctctg cgcgagcgcc tgcaccacca ccagcgccag
     3421 caccgccagc aggatggtca tgatcgtgat gattccgatg atcgcggctg ccccgccgac
     3481 cgagccgagc tcgtcgcgga ccatctgccc cagcgagcga ccccggcgcc gagtcgagat
     3541 ccacagcacc aggtagtcct gcacacagcc ggagaagacc gcgccgatga tgatccagat
     3601 ggtgcccggc aggtacccca tctgcatcgc caacacgggt ccgacgagcg gtccggctcc
     3661 ggcgatcgca gcgaagtgat ggccgaacaa cacgcgtcgg tcggttggca tgtagtcggt
     3721 gtcgttctcg aacacctctg cgggcgtggc gttgtcgtcg cgcggacgga cgatcttcat
     3781 ttcgatcaac cgcgcataga accggaagcc gatcacgtag gtgcagatcg ccgcgatgac
     3841 gaaccacacc gcgttgaccg tctccccgcg gaagaacgcg atgattgccc acgcgaccgc
     3901 gccgagcacg gcgatgatcc cgaagatgat cttgtgtctg gtggtgatcg gggaccgatc
     3961 gatgatcgct accggcggca gatccttgtc ggtgcggata taggtgacgt ctccgtcccg
     4021 ctcctcgatg cggtccgatg gtgcggcggt gggtgtagcc acggtctctc cctcgacatc
     4081 gtcgtcttac ccgttttcat gatcctttca ccgctgaaaa ggtgttgggg cgattccggg
     4141 tatcgaatcg gttatgaccc gcgctcgtag aaggcgcgga cggtgtcgat cgtgtccgcc
     4201 tcagctgggc ttttgtcgtc gcgcggacgg acgatcttca tttcgatcaa ccgcgcatag
     4261 aaccggaagc cgatcacgta ggtgcagat
//
LOCUS       DASRJA010000757        11299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2213988,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000757 DASRJA010000000
VERSION     DASRJA010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11299
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213988"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VFC01_34120"
     CDS             complement(<1..1146)
                     /locus_tag="VFC01_34120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HZN84696.1"
                     /translation="MRFTRSVERQRVDGFGRLDLGLHQAGDWYLWGPYLSERQWGTVR
                     EDYSPDGDAWNYLPHDHARSRAYRWGEDGLAGFCDIEQRLCVALALWNGHDPILKERS
                     FGLTGAEANHGEDVKEYWWYLDAVPSHAWNSWRYHYPQRAYPYQQLLDENRRRGKLDP
                     EYELLETGIFDDDRYWVIDVQYAKADPTDILMTISVTNAGPDTELLHVLPTMWYRNTW
                     SWDVDEARPTITATSTGTVAANHPFLGQLELLPGPDAHGAYPELLFCDNDTNLERLYG
                     VPSSSRWPKDGINDHVVTGADTVNPERRGTKFAAWYRLTVGPGETTELRLRLRPAGVG
                     PDAATALGRDFSRVVEQRRAEADEFYAELTPAAATSDEAAVMRQAFAG"
     gene            complement(1295..2359)
                     /locus_tag="VFC01_34125"
                     /pseudo
     CDS             complement(1295..2359)
                     /locus_tag="VFC01_34125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405125.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
     gene            complement(2490..3131)
                     /locus_tag="VFC01_34130"
     CDS             complement(2490..3131)
                     /locus_tag="VFC01_34130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN84697.1"
                     /translation="MTATQSHSGMKQAREPVQGRSVDAELSARFECEVVPLRDLLYQN
                     AFRMSKNHADAEDLVQETVMKAYTHFDSFRSGTNLKAWLLRILTNTYINGYRQKRRRP
                     VQYSTEHLTDQYLTEANARSVASALRSAEDHALDLLPDNDIKAAMQALPGQFREVVYY
                     ADVEGLHYKEIAALTNAPHGTVMSRLHRGRRQLRKLLSGSVGQVGPQALPATA"
     gene            complement(3172..3774)
                     /locus_tag="VFC01_34135"
     CDS             complement(3172..3774)
                     /locus_tag="VFC01_34135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HZN84698.1"
                     /translation="MNNLLEQKLWKWKLVAGLLTIVLGGIVLAWPGPTILVASTLFGV
                     YLLLTGFTELFLAFTLPRSAATRVLLFISGALSLVLAVLSFRHFGDAYAILLLSIWIG
                     IGFIFQGVSEVAVSIGEQKLPGRGWYAVLGIISVIAGFVVLVWPFDSIVMLTLVTGVW
                     LVVMGVVQIVQAIQIRKDAKTVREAVDSVSERFAPRKRSA"
     gene            4343..4516
                     /locus_tag="VFC01_34140"
     CDS             4343..4516
                     /locus_tag="VFC01_34140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84699.1"
                     /translation="MATHQDPGSMGVDAGSPMSTGVTVTESVAPTTPPVIRATPGITG
                     PAPLPPEEQGLPG"
     gene            4544..5695
                     /locus_tag="VFC01_34145"
     CDS             4544..5695
                     /locus_tag="VFC01_34145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84700.1"
                     /translation="MAYPSTTWVAPGTDGRLEVFAVGSDHDGGQSLWHRWQTTSADAW
                     SDWFSHGAPPDAAGLRWSPTVAAGADGYLEVFVVGDDLQPDGLGSGGALHRTSQTTRN
                     GGLSHWRSYPTGGPNLFGSPAVVRSADAHLQVFGLGDDGALWHMWQTAPNNGWSQWVS
                     HGAPPGLVLNSAPSVAAGEDGRLDVFIVSQEAALWHIAKTAINGGWSDWVSHGSPSGV
                     LFGSDSTPVVGYSADGRLELFVVGNDGRLWQMRQTVPNGGWSGWRSHDAPSGMKFLRC
                     RPAVAPGLDGRLELFLVGDDASLWHLRQTGSNDASSQWFSHGAPAVAGLVGSPAVAPD
                     ADGEMQVFVIGTDGALWQLSQTGPSGGWSQWFSHGAPEGTSLLAAITTG"
     gene            complement(5796..7730)
                     /locus_tag="VFC01_34150"
     CDS             complement(5796..7730)
                     /locus_tag="VFC01_34150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HZN84701.1"
                     /translation="MHTISESVSTPRAAGSAKTARLALVVGALGVVFGDIGTSPIYTV
                     QTLFNPGDPHPVPISTQNVYGIVSLIFWSVMIIVTLTYITLVMRADNNGEGGIMALIT
                     LVLRLGGRGRTAVVLATLGIFGAALFFGDSMITPAISVLSAVEGLKVVAPNLDEWIVP
                     MTAVIIVALFAVQRRGTAAIGRLFGPVMIAWFVAIGACGVRGIVGHPEILKALSPVYA
                     LEFMVGHFHIAFFALAAVVLAFTGAEALYADMGHFGRSAITWGWLALVLPACTLNYFG
                     QGALLLGDQATVSAPFFLLTPGWARLPMVLLATAATIIASQAVITGAFSVASQAAQLG
                     YLPRLRILHTSASRIGQIYVPWINWLLMVAVLVLVFAFRSSAALSYAYGMAVMGTITI
                     TTLLFLYVARRRWRTPLWLVVSGGGALLFVDLMFVAANLTKLVHGAWLPLVIGVAAFV
                     VMTTWQRGREVVTREREKEEGPLREFVDGLSDRKPRLLRVPGTAVFLNRGKDTAPLAM
                     RANVDRNYVLHQQVIIIAVDTVSVPRVADSERTSVDALGSANDGIIHVTANFGYMETP
                     NVPHLLRLLDPSQTEGRIAVDDASYFLSSIELVVSAAPTMSKWRKYLFIATSHLTADA
                     AEHFALPRDRTVIMGSRIDV"
     gene            complement(7888..8829)
                     /locus_tag="VFC01_34155"
     CDS             complement(7888..8829)
                     /locus_tag="VFC01_34155"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HZN84702.1"
                     /translation="MAFAVAVLKKFGDDQAGNQVALLTYYAFVATFPLLLALTTILGV
                     ALRKYPELQRDLVNSAFAEFPIIGAQIHDQLGVATFGNAFSLAVGVLGALVGGRGFAN
                     ALQNTFSTLWSVPRVDWPGFPTKYLRAGGLLLLLAVGSAVTAAAGAAAGAATASGLHG
                     LSARLISIAVGSILGWAYFLAAYRIATPARVPTRWMLPGAAISAVAWQLLLTGAGVIT
                     THLLRHAQAVAGLFGMVLGLLAWFGLQATVVVYAIEADVVRARHLWPRSLVPPPLTDA
                     DKVYYTDAVRAETQRPEQRLDIQYVPLDTAAARHEAG"
     gene            9127..10080
                     /locus_tag="VFC01_34160"
     CDS             9127..10080
                     /locus_tag="VFC01_34160"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN84703.1"
                     /translation="MNLSLVLAESASRYPDAVALRCADVTTTYSMLADDVARFADYLI
                     DGGLEPGDRVGVMLPNDAAFAVVFYGVLRAGGVVVPMSPSLRFRAVEFDSTVTDARIL
                     FYAGRHAVATELAAVTAGTQPVRVAKGGIATLIAGFAGRTGPVDRAEDDIAATFYASR
                     ATGAPKITQLTHAELARNGSVIARRLLDLGSDDIVMGCLPLFEGYGLSCGLLAAASAG
                     ATLELLSRFEPRRALEVIAAERVTVFEGVPAMYKSMLAAADRCQPDFSSLRVCMSIGA
                     SMPEDVVSRFEDRFGCMVVQATESAFIDGWLVTGALGGEAE"
     gene            10359..10787
                     /locus_tag="VFC01_34165"
     CDS             10359..10787
                     /locus_tag="VFC01_34165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84704.1"
                     /translation="MSDADLKAQFEKIADSARAATDKVKAAGERTRDQLEADVETAHE
                     KADAAADRMKDKADAARDNASSDWQELREKWHAHVAKARARAHKKKDQIDAHNAAVDA
                     DMAEAYAYDAIDFALDAIEEAEYAVLDALYTRANAEALKV"
     gene            10806..11276
                     /locus_tag="VFC01_34170"
     CDS             10806..11276
                     /locus_tag="VFC01_34170"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HZN84705.1"
                     /translation="MRDRNNGRPRTWDRQCSTPRVGDSRVDTSVARVVQGERLMQASS
                     DILLGWLRGVGPDGHEGDYYVRQLRDWKESASVESMGPQLLADYGRSCAHVLARAHAR
                     TGDRVAISGYLGSGDVADVAFTRFAEAYVRQNERDYAALREAVACGQVAAEPDL"
BASE COUNT         2174 a         3571 c         3575 g         1979 t
ORIGIN      
        1 gccggcgaac gcctgacgca tgaccgccgc ttcgtcggaa gttgctgcgg caggtgtgag
       61 ttcggcgtag aactcgtcgg cctcggcgcg ccgctgctcc accacccggc tgaagtcccg
      121 tcccagagcc gtcgccgcat ccggcccgac gccggccgga cgcaggcgga gtcgcagctc
      181 ggtggtctcg ccgggaccca ccgtcagccg gtaccaggcc gcaaacttgg tgccgcgtcg
      241 ctccggattg acggtgtcgg cgcccgtgac gacgtggtcg ttgattccgt ccttcggcca
      301 tcgtgaggaa gacggaactc cgtagagccg ttcaagattc gtgtcgttgt cacagaacag
      361 cagctccggg taggcgccgt gtgcgtcggg tccgggtaac agctcgagtt gaccgaggaa
      421 cggatggttg gcggccacgg tcccggtcga tgtggccgtg atcgtcggtc gggcttcatc
      481 gacgtcccag gaccaggtgt tgcggtacca catggtcgga agcacgtgca acagttcggt
      541 gtcgggcccg gcgttcgtca cgctgatcgt catcaggatg tcggtcggat ccgccttggc
      601 gtactggacg tcgatgaccc agtagcggtc gtcgtcgaag atcccggttt cgagcaactc
      661 gtactcgggg tccagcttgc cgcgccgccg gttctcatcg agcagttgtt gatacgggta
      721 cgcccgctgc gggtagtggt agcgccagct gttccaggcg tggctgggca cggcatcgag
      781 ataccaccag tattccttga cgtcctcgcc atgattggcc tccgcgccgg tcaatccgaa
      841 cgaccgctcc ttgaggatcg ggtcatgccc gttccataag gccagcgcca cgcacagccg
      901 ctgctcgatg tcacaaaagc cggcgagccc gtcctcgccc cacctgtacg cgcgcgagcg
      961 cgcgtggtcg tgcggtaggt agttccaggc gtcaccgtcg gggctgtaat cctcacggac
     1021 tgtgccccat tgcctttcgc tgaggtacgg gccccacaga taccagtcgc cggcctggtg
     1081 cagacccagg tcgaggcgac cgaagccgtc cacccgctgc cgctccacgc ttcgtgtgaa
     1141 tctcacgtca tcggaccgtg ccgcctcgtt ggacatgtgt cctcctgaat acgtagatgc
     1201 gttcggcgca tcttcgcgcc gacatcgctc gccgtcattg ttggagcgga cgagatgcaa
     1261 acggcgactg tgtgccggca aacacagact acgactaccg cccaccctgg tcagtgccga
     1321 gcgtgaccag gacgctcctg ggatcacccg aaggatcaat gaatcccaca gtcactcgag
     1381 cacctggggc ctgtgactgc actgccgcgc acagcgcgcc ggcgtctggg atcacttggt
     1441 catcgacctt cgtgaccagc gcgccattag gcagcccggc tacggccgcg ggactaccgc
     1501 ttgtcacacc aacgaccctg gcgccctggg cgtccatctc agtgcctacc tgcgcaccta
     1561 gccaagcatg cgatgccgta ccggtggcga tgagttcact ggcgatgcgc gcggcgtgat
     1621 ctacgggaat ggcgaaacca atcccgatcg acccgctctg cgcatcggcg tcgtcgccaa
     1681 gatgacccag cgacgccgtt gccgaattca ttccgatgag cttgccgttc atgtcaacca
     1741 gggcgccgcc cgagttgccc gggttgagcg cggcgtcggt ttggatggcg tcaaaagccg
     1801 cggactgagt gttgccgtcg gcggcgccga ccacaggacg gtctagtgca ctgacgatgc
     1861 caacggtgac ggtgcccgtc aggccgagcg gagatccgac ggcggcaacc ggttgaccga
     1921 cacgaaggtc ggccgaggag ccgagcgaga tcggagttag ccccgaaatc ccttgagccc
     1981 gcacgaccgc gacatcactc tcggtatcgg tggcgaccac gctgaacggg acggtacgac
     2041 cgtcgttcaa cttgaccagg cttctgacgg gctcctgcga cgcaccaccg gccgccgcga
     2101 cgacatggtt attggtcatg atcagcccat cagaggtgag gatgatgccc gacccctcgt
     2161 gaaactcgcc gccgcccagt tcggttgtca atgtcaccac gctgggcaac accttggtgg
     2221 cgagttgttc gaccgagggg ttgaccgtcg actgcgcctg tgcgtacgag gggacggcca
     2281 gttgcctgtc gatgccgggc gggagagcga gcaaagcgac ggctgcaccg accgccgccg
     2341 aaactgctgc caatgcgagg ccacgcaaaa tcagcgatcg aaaaggcggt cgccgccgcg
     2401 gcgtcgctat cgcccgatgt gacgggtatg gggagggttg cttttgatgc ggcgaatgcc
     2461 ggcgatcgtg ctcaacgtca ggcattggat cacgcagtcg cgggcagtgc ttgggggcca
     2521 acttggccga cgctgccact gagcagcttg cgcaattgtc gtcgaccgcg atgaagccgc
     2581 gacatcacgg ttccgtgcgg ggcgtttgtc agagccgcga tttccttgta atggagcccc
     2641 tcgacatcgg cgtaatacac gacttcgcga aattgccccg ggagcgcctg catcgcggcc
     2701 ttgatgtcgt tgtccggcaa taaatccagt gcgtgatcct cagccgaacg caaggccgat
     2761 gccaccgagc gtgcgttggc ctcggtcagg tactgatcgg tgaggtgctc ggtggagtac
     2821 tgcaccggtc gacgtcgctt ctggcggtag ccgttgatat aggtgttggt caggatccgc
     2881 aacagccatg ctttcaggtt ggtgcccgac cggaatgaat cgaaatgggt gtacgccttc
     2941 atcacggttt cttgcaccag gtcctcagcg tccgcgtgat tcttgctcat gcgaaacgcg
     3001 ttttggtaga gcagatcgcg cagcggaacg acctcacatt cgaaccgtgc actcagctcg
     3061 gcgtcgaccg accggccctg caccggttcg cgggcttgtt tcatccccga gtgggactgc
     3121 gtcgcggtca tggtcatcct tccgaaggtt gattcgccgg acctggcggg gctacgcgga
     3181 tcgctttcgt ggtgcgaacc gctcagacac agagtcgact gcttctcgca cggtctttgc
     3241 gtccttgcgg atttgaatcg cctgaacgat ctggaccacg cccatgacga caagccacac
     3301 gcccgtgaca agtgtcagca tgacgattga atcgaacggc caaacaagaa cgacgaagcc
     3361 ggcgatcaca gagatgatgc caaggacggc ataccaaccc cgcccgggca atttctgctc
     3421 accgatcgag accgcgacct ccgaaacacc ttggaagata aacccgattc cgatccagat
     3481 tgacagcagc agaatggcgt aggcatcgcc gaagtggcgg aaagacagca cggccagaac
     3541 caacgacagt gcgccactga taaacagcag aacgcgagtg gcggcggacc gaggcaaggt
     3601 gaacgccaga aacagttctg tgaacccggt cagtagcagg tagacgccaa ataatgtcga
     3661 ggcgacaaga attgttggac caggccaggc cagcacgatg ccgcccaaga ctatggttag
     3721 caggccagcg acgagtttcc acttccacag cttctgctca agaaggttat tcatggcact
     3781 ccctcgtttc gtcgaaagta tcggtcttgc ggcacgccca tctgcagtcg ttggaggtag
     3841 acggataccg ttgccgacgt tgctttttcg tcggcaactt gattctctag cgagttcacc
     3901 gaagtgtcga tgtcgtccgg gacaacccgg cgttgcaatt cgtgtcaccg ctaacaccaa
     3961 cgccaggcgc aaggcgcact aacagcactt caccactggc gcgctgttgg cgccgggacc
     4021 gtgttaccac ggacaactct cggtcgcgat cgttgtgacc agttacatcg acggcgtcgg
     4081 tggctcgtac aacgatggag ttacttggct cagaagtgat tcgggacacc cagttctgac
     4141 cacaatggac cgcctgttcc gtgtcgatat cagtgacaac cgcgtcgtgt agcccaatcc
     4201 ggaaacgtat cccgatggca tcgcggtcga tgtcacgcgc gacacattta ctgcaaatag
     4261 aggaaatgac atgagcgaca caatgatccg gtcaattgct gtgattggag taagcgctgt
     4321 tgtcacggtt ggcgctgtcg ccgtggctac acatcaggat cccggcagca tgggcgtcga
     4381 cgcgggctcc cccatgtcca ccggggttac cgtcactgaa tccgtggcac cgacaacacc
     4441 tcccgtgatc cgggcgacgc cgggcatcac gggccccgcc ccgctgccac ccgaagaaca
     4501 gggtttgccc ggatagaccc ctgctcgacc tgaaggaccc aacatggcat acccatcaac
     4561 cacatgggtc gcccccggga cagacgggcg gctggaggtc ttcgccgtgg gcagcgacca
     4621 cgacggcggc caatcactct ggcaccgatg gcagaccacc tcggccgacg cctggtcgga
     4681 ttggttctca cacggtgccc cgcccgatgc agctggtctg cgctggtccc ccaccgtcgc
     4741 agcgggcgca gacgggtacc tggaggtctt cgtcgtcggt gacgatcttc aacccgacgg
     4801 cctgggatcc ggtggagcgc tgcacaggac atcgcagacc acccgcaacg gtgggttgtc
     4861 ccattggcgt tcatacccga cgggcgggcc gaacctgttc gggtcgccgg ccgttgttcg
     4921 cagtgccgac gcccacctgc aggtgtttgg gttgggcgat gacggagcgc tgtggcacat
     4981 gtggcagaca gctcccaaca acggctggtc ccagtgggtc tcgcatggcg caccccccgg
     5041 gctcgtcctg aattccgcgc cgtcagttgc tgccggggaa gacggacgct tggacgtgtt
     5101 catcgtgagc caggaagcgg cgctgtggca catcgcgaag acggctatca acggtggctg
     5161 gtctgattgg gtttcgcacg ggagtccctc cggtgtgctg ttcggctcgg actccacgcc
     5221 ggtggttggc tacagcgcgg acggacgcct ggagctgttc gttgtcggca acgacggaag
     5281 gctatggcag atgcggcaga ccgtccccaa cggcggctgg tcgggctgga gatcacacga
     5341 tgccccatcg ggcatgaagt tcctacgatg tcggccggcg gtcgcccccg gcctggacgg
     5401 acggttggaa ctattcctcg tcggcgacga tgcatcgcta tggcacctgc gccaaacggg
     5461 atccaatgat gcctcgtcgc agtggttttc gcacggagct ccggcggtgg ccgggttggt
     5521 ggggtcgccg gcggtcgctc ccgacgctga cggcgaaatg caggtctttg tcataggcac
     5581 cgacggagcc ctgtggcagc tgtcgcagac cggtcccagc ggcggatggt cgcagtggtt
     5641 ttcgcacggg gccccagagg gaacttcgct tcttgcggcg atcaccaccg gttgagcgcc
     5701 gcctcaagga gattcgggcg cagatacacc aggggtgcat tcttgaaacg ccagggaaca
     5761 ctccatcgcc cttggtggac tctcaatcag ccgggctaca cgtcgattcg cgagcccatg
     5821 atcaccgtgc gatcccgcgg tagggcgaaa tgctcggcgg cgtcggcggt cagatgggac
     5881 gtggcaatga acaggtactt gcgccacttg gacatcgtcg gcgccgcact cacgactagt
     5941 tcgatgctcg agaggaagta ggaggcgtcg tcgacagcaa tgcggccttc cgtctggctc
     6001 gggtcgagca gtcgcagcaa gtgcggtacg ttcggcgttt ccatgtaccc aaagtttgcg
     6061 gtcacgtgga tgatcccgtc gttggcgctg ccgagcgcat cgacgctcgt gcgttcagag
     6121 tccgctacac gcggcaccga cacggtatcg accgcgatga tgatcacctg ttggtggagc
     6181 acataattgc ggtcgacgtt cgcccgcatc gccagcggcg ctgtgtcctt cccgcggttg
     6241 aggaacaccg cagtgcccgg cacccgtaat aaccgcggct tacgatccga gaggccatcg
     6301 acgaactcgc gcagcggccc ctcctctttc tcgcgctccc gggtgaccac ctcacgtccg
     6361 cgctgccaag tcgtcatcac aacgaacgcc gcgacaccga tcaccagagg cagccacgcc
     6421 ccatggacca gcttggtcaa attggccgcg acgaacatca ggtcaacgaa tagcaacgca
     6481 ccgccgccgc tcaccacgag ccacagcggg gtccgccatc gtctccgggc cacgtaaagg
     6541 aacagcagcg tggtgatggt gatcgtgccc attaccgcca tgccgtacgc gtatgacagt
     6601 gccgccgagc tacggaacgc gaacaccagg accagcaccg ccaccatcag caaccagttg
     6661 atccacggaa cgtagatctg gccgatcctc gacgccgagg tgtgcagtat tcgcagcctc
     6721 ggcaagtacc cgagctgagc ggcctgagac gccactgaaa aggccccggt gatcacggcc
     6781 tgggaggcga tgatcgttgc agcggtggcc aatagcacca tcggtaatcg agcccaaccc
     6841 ggagtgagca gaaagaacgg agcactcacc gtggcctgat cgccgagcag caatgccccc
     6901 tgaccaaagt agttgagcgt gcacgccggc aacacgaggg caagccagcc ccaggtaatc
     6961 gccgaccggc cgaagtgacc catgtcggcg tagagcgcct ccgcaccggt gaacgccagc
     7021 accacagcgg ccagtgcgaa gaaggcaata tgaaagtgtc ccaccatgaa ttccagcgca
     7081 tacaccggcg agagcgcctt gaggatctcc ggatggccga cgatgccgcg cacaccgcac
     7141 gcgccgatcg ccacgaacca ggcgatcatc accggcccga acaggcggcc aatcgcagcc
     7201 gtgcctcgcc gctggacggc gaacagggcg acaatgatca ccgcggtcat cggcacgatc
     7261 cactcgtcca gattcggcgc aacgaccttg aggccctcga ccgcggacag caccgagatc
     7321 gccggggtga tcatgctgtc gccgaagaac aacgctgcac cgaagatgcc cagtgtggcc
     7381 agtaccacgg ctgtgcggcc ccttccgccc aatcgcagca cgagtgtgat gagcgccatg
     7441 atgccgccct cgccgttgtt gtcggcgcgc atcacgagag tgatgtaggt cagcgtgacg
     7501 atgatcatca ccgaccaaaa gatcagtgac acaatgccgt acacgttctg cgtgctgatg
     7561 ggcaccgggt gcgggtcgcc tgggttgaag agtgtctgaa cggtgtagat cgggctggtg
     7621 ccgatgtccc cgaacacgac cccgagcgca ccgacaacaa gggccaaccg cgccgttttc
     7681 gcactccccg ctgcgcgggg cgtcgacacc gattcggaaa ttgtgtgcat tccacccacc
     7741 tccgttccac accagaaccc cagctcagtg acccgagtct tcaggacatc gggtcctcca
     7801 gcgaggtcgc ccgagacaac gtcgctcgag tcttgctgta ggcgtggaca acgccgctgc
     7861 gccgcacgga aatcgagcgc ttcgcgatca acccgcctcg tgccgcgcgg cagcggtgtc
     7921 caatggcacg tattggatat cgagccgctg ctcgggccgt tgagtctcgg ccctgaccgc
     7981 gtcggtgtaa tagaccttgt cggcgtcggt cagcgggggt ggaacaaggc tgcggggcca
     8041 cagatgtcgg gcccgaacga catctgcctc aatcgcatac acaaccaccg ttgcttgtag
     8101 accgaaccat gcgagcaacc ccagcaccat gccgaacagg cctgcgacgg cttgggcgtg
     8161 gcgcaacaga tgagtggtga tgacgccggc tccggtcagc agcagctgcc aggcgaccgc
     8221 gctgatcgcc gccccgggca gcatccagcg tgtcgggacc cgtgccgggg tggctatccg
     8281 gtacgccgcg aggaagtagg cccagcccag gatggagccg accgcgatgc tgatcagacg
     8341 cgcactcaac ccgtggagtc ccgaggctgt cgccgcgccg gcggctgcgc cagcggcggc
     8401 cgtcaccgcc gaaccgacag ccagtagtag tagcaggccg cccgcccgca ggtacttggt
     8461 gggaaacccg ggccaatcca ccctaggcac cgaccagagc gtgctgaacg tgttttgcag
     8521 agcgttggcg aaaccacggc cgccgacaag ggcgcccaat acaccgactg caagcgaaaa
     8581 ggcgttgccg aacgtggcaa cgccgagttg atcatggatc tgggcgccga tgattgggaa
     8641 ctcggcgaat gccgaattca ccaggtcacg ttgaagctcc gggtatttcc gcaacgccac
     8701 accaaggatc gtggtcagcg cgagcagaag cggaaaggtc gcgacgaagg cgtagtacgt
     8761 caacaacgcc acttgattgc cggcttgatc gtcgccgaac ttcttcagca ccgcaacggc
     8821 gaacgccatc agtggatgtc gctgctgcca gctatcgaac cggcgcgcca ggcgctcaac
     8881 gcgattcacc ggtgcgccag ttacgtttga tcgctgatgc ggcccatggt tgtcgagaat
     8941 agaacgactc ggagatttca gcatcaaccc cgcgctgttc tcgaaatcaa ttgtcgccgt
     9001 ggccaatctg aggtcaccca tcgtgtcacg tcgaacaatg aaatctcgac actcaccaac
     9061 gacgatgcac gaagtgtcgg tgcgaacccg atcgacacac ggactcgcaa ccgcggagtg
     9121 gtcacgatga atttgagcct tgttctcgcc gagtcggcgt cacgataccc ggatgccgtc
     9181 gcgctgcgat gcgccgacgt gacgacgacg tattcaatgc tggccgacga tgttgcgcgc
     9241 ttcgccgact acctcatcga tggtggtttg gaacccggtg atcgggtagg cgtgatgctg
     9301 ccaaacgacg ctgcgttcgc cgtggtcttc tacggagtcc tgcgcgcagg aggggttgtg
     9361 gtgccgatga gcccgtcgct gaggttccgt gcggtggagt tcgattcgac ggtcaccgac
     9421 gcacggatat tgttttacgc cggtcgacac gcggtggcga ccgagctcgc cgcggtaacc
     9481 gccggcacgc aacccgtgcg agtggcaaag ggtgggatcg ccaccctgat tgccgggttc
     9541 gcaggccgca ctgggccagt ggatcgtgcc gaagacgaca tcgcggccac cttctacgcc
     9601 tccagggcga cgggtgctcc aaagatcacc caactcacac acgccgaact cgcccgcaac
     9661 ggatctgtca tcgcgcgccg ccttctggac ctcgggtccg atgacatcgt gatgggatgt
     9721 ctgcccttgt ttgagggtta cgggttgtcg tgcggtttgt tggccgcggc ctccgcaggg
     9781 gcaactctgg aattgctgtc gcggttcgaa ccgcgtcggg cgctggaagt aattgccgct
     9841 gaacgggtaa cggtcttcga gggcgtgcct gcgatgtaca agtcgatgct cgccgcggcc
     9901 gaccgttgcc agccggattt cagttcccta cgcgtctgta tgtccatcgg tgcctcgatg
     9961 cccgaggacg tcgtgagtcg gttcgaggac aggttcggct gcatggtcgt tcaggccacc
    10021 gagtccgcct tcatcgatgg gtggcttgtc accggcgcct tgggcggaga agcggaatga
    10081 gcatgccggg gaccctcgcc gacaaggtga tttcgcgtac cgggtaaagg ccatgcaact
    10141 cgcgggagac gcttgatcgc tgatggagcc catgattgtc gacgatcgaa cgaggcggac
    10201 gttgcaacat caacgcccgc cggtatcgaa atcgatgatc gccgtgacta atctgcggtc
    10261 actgatcgtg tcgcgtcgaa caatgactcc tcgacaccca ccacggacga tgcacggtag
    10321 aggcggatag cccgccttgg gtggacaaag gagctttcat gtcagacgcc gacctcaagg
    10381 cacagttcga gaagatcgcc gactcggcaa gagccgcgac ggacaaggta aaggcggcgg
    10441 gtgagaggac acgggatcag ctggaagctg acgtcgagac tgcacacgaa aaggccgacg
    10501 cggctgccga tcggatgaag gacaaggcgg atgccgcccg ggacaacgcg tcatccgact
    10561 ggcaggagct tcgcgagaag tggcatgccc acgtcgccaa ggctcgggca cgcgcgcaca
    10621 agaagaagga ccagatcgac gcacacaacg ccgcggtgga cgccgatatg gccgaggcgt
    10681 acgcatacga cgcgattgat ttcgccctgg acgcgatcga agaagccgag tacgccgtgc
    10741 tcgatgccct atacacacga gccaacgccg aggcgctcaa agtctgagcc tagaatccat
    10801 cccaggtgcg ggaccgcaac aacggccggc cccgcacctg ggatcggcaa tgcagcaccc
    10861 cccgggtcgg tgactcccgc gtggacacat ccgtagcgcg agtggtgcag ggtgaacggc
    10921 tgatgcaggc cagcagcgat atcctgctgg gctggctgcg aggcgtcggc ccagacggcc
    10981 acgagggcga ctactacgtg cgtcagctgc gcgattggaa ggaatcggcg agcgtggaat
    11041 cgatgggtcc gcagctactt gccgattacg gtcgctcatg cgcacacgtc ctcgcgcgcg
    11101 cgcatgcccg cacgggtgat cgggtcgcca tttccggcta tctcggtagc ggtgacgtcg
    11161 ccgacgtcgc gttcacccgg ttcgccgagg cctacgtccg acagaacgag cgcgactacg
    11221 cggccctgcg cgaggccgtc gcctgcggac aggttgccgc cgagcctgac ctctaagtct
    11281 tcatcaatga tgacggccc
//
LOCUS       DASRJA010000758         7126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_677680,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000758 DASRJA010000000
VERSION     DASRJA010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677680"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1275)
                     /locus_tag="VFC01_34175"
     CDS             complement(112..1275)
                     /locus_tag="VFC01_34175"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN84706.1"
                     /translation="MSSGPLSGVLVVDLTAMVMGPYCTQIMADMGADVIKIEPPEGDN
                     TRYISVGPAPGMSGVFVNVNRGKRSVVLDLRSEPGKTALRALVLEADVFIHSMRSKAI
                     AKLGFGYEQVAALNPRIIYTNCYGYGRRGPQRDLPAYDDTIQAECGIPAVQEQLTGEA
                     NYVGTIMADKVAGLTALYATMMALFHRERTGEGQEVEVSMFETMASFMLVEHANGAMF
                     DPPLGPAVYPRTVAPNRKPYRTSDGYIAALIYNDKHWNAFIDAVQPNWASDLYGTLEL
                     RARSIDTVYGLVAETMKKRTTAEWLALFRELQIPAAPLNTPDALFDHPHLNAVGLFET
                     VDTPHGPVRFPGVPTWFSCTPGRVAGPAPLLGEDTEQVLGELGLADVEAAESV"
     gene            complement(1268..2191)
                     /locus_tag="VFC01_34180"
     CDS             complement(1268..2191)
                     /locus_tag="VFC01_34180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN84707.1"
                     /translation="MPDLRFDDRVAVVTGGGRGLGRSYALLLASQGAKVVVNDPGGSI
                     GGDGTDAAPADEVVREITAAGGQATASTESVATPEGGKAIIDAALDAYGRVDILVHNA
                     GNVRRASLKEMSYDDFEAVLDVHLRGAFHVVRPTFPQMCDAGYGRIVLTSSIGGLYGN
                     HDVANYAVAKAGVIGLSNVAAIEGAPHGVKSNVIVPAAVTRMAEGIDTSAYPPMGPEL
                     VAPAVAWLSHESCSVTGEMLVALAGRVAMAVVVETPGVYRPSWSIAEVGEHIAAIREA
                     SAPVLFPVVPDGHGDHIRYSFAMATQEAHRV"
     gene            complement(2279..4123)
                     /locus_tag="VFC01_34185"
     CDS             complement(2279..4123)
                     /locus_tag="VFC01_34185"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN84708.1"
                     /translation="MTLFDTCGPTQTPDDIDIDALRDKYLYERDKRLRAEGSKQYLEL
                     KGEFAEFAEVDPHTPVTPRAPMSEDIEVAVLGGGIAGLLAGTYLKKAGVDDVHVIEMG
                     GDFGGVWYWNRFPGIQCDNDAYCYIPMLEELNFMPSKKFADGAEIYEHCRRIAKHFGL
                     YDGAILSTQVRTVRWDESIKRWRLSTNRGDDIRARFVVMTPGSYNRPKLPGIAGIKDF
                     KGHVFHSARWDYDYTGGDASGGLHKLADKRVALVGTGATGVQLVPHLGRDAKHLYVFQ
                     RTPSSVDERGNAPTDQEWVKSLQPGWQEERKRNFHRWSPFEGVVFDAADQVCDFWTEL
                     GRNMTARIAASEDPASLGIEQIMAIREEEDYKIMERLRRRIAAVIDDPETAEALKPYY
                     RFLCKRPCSSDEYLPTFNRPNVTLVDVSASKGVERLTQNGVVANGVEYQVDCVIFASG
                     FEISTEISRRYAMDVIEGREGLSLFDNWRDGYKTLHGMTSRGFPNQFHTGFIQGGVSA
                     NTTAMFEQQAKHIAYIISEAVSRGATTVEPSQEGQDDWVSTIRELSMDNSAFEVSCTP
                     GYYNNEGGGGGEGIRSFLGEPYSPGFYAFEDLLIQWRDKGDLNGLVLK"
     gene            complement(4127..5488)
                     /locus_tag="VFC01_34190"
     CDS             complement(4127..5488)
                     /locus_tag="VFC01_34190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011782106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZN84709.1"
                     /translation="MTDTEVQAAEELSEPVTIGVEAYISEDYARAERDKLWRKVWQQV
                     GRVEELPEVGSYLTYDILDDSILVVRTGPNEFTAHHNVCMHRGRRLVDTPEGEKNARG
                     RARKSFVCGFHGWTYGLDGACTHIREQDDWKGQLTPRNTHLAPVQVDTWAGWLFINMD
                     PDCEPLADYLFPAAKILDPFGLENMRYKWRKWLYFDCNWKVAMEAFNETYHVFTTHPE
                     FNKFGEFKGWAKAQGKHSNIGYDAPKGLDETKSKIRLGTGDPRISTAEMQVYTMEETN
                     ATTTQTLVNAAKRLVDELPEGTPADKVLEHWLSSARRDDEARGVIWPTIPADILGQSG
                     TAWQIFPNFQIGQGLTSALCYAARPDPSYDPNKCIFEVAVFELYPKGQEPQTEWQYTP
                     KESPNWLSVLPQDFSNMAAVQQGMKSLGFPGTKPNPYRERSTVNLHYQLSKYMGTGEP
                     REL"
     gene            complement(5485..6342)
                     /locus_tag="VFC01_34195"
     CDS             complement(5485..6342)
                     /locus_tag="VFC01_34195"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN84710.1"
                     /translation="MDDLLKLDGRIVVVSGAGGGGIGTTVTRLVAEAGATVVAVSRSK
                     ENLDEHVSPLAQRGLSVVPVAADASTDDGIATVIEHVRRTDGKLYGLVNVAGGADPST
                     WMPSTRVTRDDWRALFTRNLETAFFMSQAVAREIRQHGDPGSIVSISSISGMNTAPFH
                     IAYGTAKAAVAAMTRTMAVELAPDNIRVNAVAPGVTETAASRTYTGEDPDRDRQAIAM
                     GRRGRPEEQAGAILFLLSDLSSYITGQTLLVDGGLNLKWTHLGADNTSLFLKDDNFRR
                     AISRHEEEA"
     gene            6487..>7126
                     /locus_tag="VFC01_34200"
     CDS             6487..>7126
                     /locus_tag="VFC01_34200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZN84711.1"
                     /translation="MTSVLQARYSAANPTLADGWRLERLTPPSRLFGANGLRTGPDGR
                     VYVAQVTGSQISALDLKTGQLDTVSAKGGDIIAPDDVAFDPRGNLYATEVMDGRVSVR
                     EPGGTTRVLRDDLPCANGITVHAGRLFINECRDGGRLMELDGSGSIVRMLAENLPSPN
                     AMEVGPDGMLYYPLMTANEIWRIDPNGGEPQRVAGDLGVPDAVKFDSEGFIVS"
BASE COUNT         1184 a         2317 c         2260 g         1365 t
ORIGIN      
        1 cgggattatc tgtgaaagcg cccaaatagt gttcgggcac atgggctttc actagttcgc
       61 gcagctcgtt gcgaagctcc gcggctttct gccccatctc gaattccatg cttacaccga
      121 ctccgcagct tcgacgtcgg ccagaccaag ctcgccaagc acctgctcgg tgtcctcgcc
      181 cagcagcggc gcgggccctg cgacccgccc cggcgtacat gaaaaccagg taggcacacc
      241 gggaaaccgc acggggccgt ggggggtgtc caccgtttcg aacaggccga cggcgttcag
      301 gtgcgggtgg tcgaacaacg catcgggcgt gttgagcggt gcggcgggga tctggagttc
      361 acggaacagc gccaaccact ccgcggtggt gcgcttcttc atggtctcgg ccaccaaccc
      421 gtacacggtg tcgatgctgc gggcacgcag ttccagcgtg ccgtagaggt cgctggccca
      481 gttcggctgc acggcgtcga tgaacgcgtt ccagtgcttg tcgttgtaga tcagcgcggc
      541 gatgtagccg tcgctggtgc gatacggctt gcggttgggc gcgaccgtcc gcggatatac
      601 ggccggaccg agcggaggat cgaacattgc cccgttagca tgctcgacga gcatgaacga
      661 cgccatggtt tcgaacatgc tgacctcgac ttcttggccc tcaccggtgc gttcccggtg
      721 gaagagcgcc atcatcgtgg cgtagagcgc ggtcagtccg gccactttgt cggccatgat
      781 cgtcccgacg tagttggcct cgccggtcag ttgctcctgc accgcaggga tcccacactc
      841 cgcctggatg gtgtcgtcat acgcgggcag atcccgttgc ggcccgcgcc ggccatagcc
      901 gtaacagttg gtgtagatga tgcgagggtt cagcgcggct acttgttcgt aaccgaagcc
      961 gagcttggcg atcgccttgg accgcatgga gtggatgaag acgtcggcct ccagcacgag
     1021 tgcgcgaagc gcggtcttgc ccggctcgga tcgcagatcc aacacgacgc tgcgcttgcc
     1081 gcggttcacg ttcacgaaca cgccgctcat tccgggagcg ggcccaaccg agatatagcg
     1141 ggtgttgtcc ccttctggcg gctcgatttt gatcacgtcg gcgcccatgt cggccatgat
     1201 ctgcgtgcaa tacggcccca tcaccatggc ggtgaggtcg acgaccaaca cgcccgaaag
     1261 tgggccgcta gacacggtgc gcctcctgcg tggccatcgc gaagctatag cggatgtgat
     1321 cgccgtgccc gtcgggaacg acggggaaga ggacaggcgc cgacgcctcc cggatcgccg
     1381 cgatgtgttc gcccacctcc gcgatggacc acgacggccg atacaccccg ggcgtttcca
     1441 cgacgacggc catggcaacc cgacccgcca atgcaacgag catctcccca gtgacagaac
     1501 aggattcgtg cgacaaccac gcaaccgcgg gtgccaccag ttccgggccc atcggtggat
     1561 aggcggaggt gtcgatgcct tcggccattc gggtaaccgc cgcgggcacg atcacgttgc
     1621 tcttgacgcc gtgcggcgca ccttcgatcg cagcgacgtt cgacagcccg atcacgcccg
     1681 ccttggccac cgcatagttc gccacgtcgt ggttgccgta gagtccaccg atcgacgacg
     1741 tcaacacgat gcggccataa ccggcatcgc acatctgcgg gaacgtaggg cgcacaacat
     1801 ggaaggcgcc gcgcaggtgc acgtcgagga cggcctcgaa gtcgtcgtac gacatctcct
     1861 tcagcgacgc ccgacgaacg tttccggcgt tgtgaacgag gatgtcgacg cgtccgtacg
     1921 cgtccagcgc cgcatcgatg atcgccttgc ccccttcggg ggtcgccacc gattcggtgc
     1981 tggctgtcgc ctgcccaccc gccgcggtga tttcgcgaac cacctcgtcg gcaggcgccg
     2041 cgtcggtgcc gtcgccgccg atgctgccgc cggggtcgtt gacgacgacc ttcgcgccct
     2101 gcgatgccag cagcagggcg tatgagcggc ccaatcccct gccgcctccg gtgacgaccg
     2161 cgacccggtc gtcgaatctc aagtcaggca tctggagttc tcgcgcccag tggccagtta
     2221 ttgcacgctt ttcggcgttc aagcgtggca taactggcca ctcgaccact atgaaaactc
     2281 acttcagcac caggccattc agatcaccct tgtcgcgcca ctggatcagc aggtcctcga
     2341 acgcgtagaa gccgggcgag taaggctcgc cgaggaatga gcggatgccc tctccgccac
     2401 caccgccctc attgttgtag tagccgggag tgcaagaaac ctcgaacgcc gagttgtcca
     2461 tcgacagttc gcgaatggtg ctgacccaat cgtcctggcc ctcttgactc ggttcgacgg
     2521 tcgtagcgcc ccgactcacg gcttcggaga tgatgtacgc gatgtgcttg gcctgctgct
     2581 cgaacatcgc ggtggtgttg gccgagacgc cgccctggat gaagcccgtg tggaactggt
     2641 tggggaaacc gcggctggtc atcccgtgca gcgtcttgta gccgtcgcgc cagttgtcga
     2701 agagtgacag gccttcgcgt ccttcgatga cgtccatcgc ataacggcgg ctgatctccg
     2761 tggagatctc gaagccgctc gcgaagatga cgcaatcgac ctggtattca acgccgttgg
     2821 cgacgacccc gttctgggtc agccgctcca cacccttcga cgccgacacg tcgaccagcg
     2881 tgacgttggg acgattgaac gtcggcaggt actcatcgct cgagcacggc cgtttgcata
     2941 ggaaccggta atacggcttg agcgcctcag ccgtctcggg gtcatcaatg acggcggcga
     3001 tgcggcggcg cagccgttcc atgatcttgt agtcttcctc ttcccggatc gccatgatct
     3061 gctcaatgcc cagcgacgcg ggatcctcgc tcgccgcaat ccgcgcggtc atgttgcgcc
     3121 ccaactcagt ccagaagtcg cacacctgat ccgccgcgtc gaagaccaca ccttcgaacg
     3181 gtgaccaccg atggaagttg cgcttgcgct cctcctgcca gcctggctgc agcgacttaa
     3241 cccactcctg atcagtcggc gcgttgccgc gttcgtcgac ggacgagggg gtgcgctgga
     3301 atacatacag gtgcttggca tcccggccga gatgcgggac cagttgcacg ccggtggcac
     3361 cggttccgac gagcgcgacg cgcttgtctg ccagcttgtg caggccgccg ctggcgtcgc
     3421 cgccggtgta gtcgtagtcc cagcgcgccg agtggaagac gtgtcccttg aagtccttga
     3481 tcccggcgat ccccggtagc ttcggccggt tgtaggagcc gggcgtcatg accacgaagc
     3541 gggcacggat gtcgtcgccg cggttggtgc tcaatcgcca gcgtttgatc gactcgtccc
     3601 agcgcaccgt gcgcacctgc gtggacagga ttgcgccgtc gtacagtccg aaatgcttgg
     3661 cgatgcgccg gcagtgttcg tagatctcag ccccgtcagc gaacttcttc gacggcatga
     3721 agttgagttc ttccaacatc gggatgtagc agtaagcatc gttgtcgcac tggattcccg
     3781 ggaagcggtt ccagtaccac acaccgccga agtcgccgcc catctcgatg acatgcacgt
     3841 cgtcgacgcc ggctttcttg aggtacgtgc ccgccagcag cccggcgatg cctccgccta
     3901 gcacggcgac ctcgatgtcc tctgacatcg gcgcccgagg cgtcaccggc gtgtgcggat
     3961 cgacctccgc gaactccgcg aactcaccct tgagttccag atattgcttg gatccctctg
     4021 cgcgcaaccg cttatcgcgc tcgtacaggt acttatcccg cagggcgtcg atgtcgatgt
     4081 catctggcgt ctgcgtcggt ccgcacgtgt caaacaaggt catatctcag agctctcgtg
     4141 gttcgccggt gcccatgtac ttggacagtt ggtagtgaag gttgactgtg ctgcgttcgc
     4201 gatacgggtt cggtttggtt ccagggaagc cgagcgactt catgccctgc tgcactgcgg
     4261 ccatgttcga gaagtcctgt ggcagaacgg acagccagtt aggactttcc ttgggcgtgt
     4321 actgccattc cgtctgcggc tcttggcctt tgggatacag ctcgaacacc gccacctcga
     4381 agatgcactt gttggggtcg taactcgggt caggccgtgc ggcgtagcac agcgcgctgg
     4441 tcaagccttg gccgatctgg aagttcggga agatctgcca cgcggtgccg ctctgcccga
     4501 gaatgtcggc cgggatcgtc ggccagatca ccccgcgcgc ttcgtcgtcg cgtcgcgccg
     4561 aggacagcca gtgttcgagc accttgtcgg ccggcgtgcc ctcgggcagc tcgtcgacga
     4621 gccgtttggc cgcgttcacc agcgtctgag tggtggtcgc gttggtctct tccattgtgt
     4681 agacctgcat ctcggctgtc gatatgcgcg ggtcgccggt gccgagccga atcttcgact
     4741 tcgtctcgtc caaacccttc ggcgcgtcgt agccgatgtt gctgtgcttg ccctgcgcct
     4801 tggcccaacc cttgaactcg ccgaacttgt tgaactcggg atgcgtggtg aacacgtggt
     4861 aggtctcgtt gaaggcctcc atcgcgactt tccagttaca gtcgaagtac agccacttgc
     4921 gccacttgta gcgcatgttc tccagcccga acgggtcgag gatcttcgcg gcgggaaaca
     4981 ggtagtccgc caacggttca cagtcgggat ccatattgat gaacagccag ccagcccagg
     5041 tgtcgacctg gacgggagcg agatgggtat tccgcggggt cagctggccc ttccagtcgt
     5101 cctgctcacg gatgtgcgtg cacgccccgt cgagcccgta cgtccagccg tggaagccgc
     5161 agacgaacga cttgcgggcg cgtccgcggg cgttcttctc gccttcagga gtgtcgacga
     5221 gccggcggcc gcggtgcatg cacacgttgt ggtgtgccgt gaattcgttt gggccggtgc
     5281 gcaccaccaa gatcgagtcg tcgagaatgt cgtaggtgag atagctgccg acttccggta
     5341 gttcttcgac gcgaccgacc tgttgccaga cctttcgcca cagtttgtcg cgttccgcgc
     5401 gggcgtagtc ctccgagatg taggcttcca ccccgatcgt cacaggttcc gaaagctctt
     5461 cggctgcttg gacttcggtg tccgtcatgc ttcctcctcg tgccgactga tggctctgcg
     5521 gaagttgtcg tccttcaaga acagtgaagt gttgtcggcg ccgaggtgcg tccacttgag
     5581 gttgagaccg ccgtcgacga gcagggtctg tccggtgatg tagctcgaca gatccgacag
     5641 caggaacagg atggcgccgg cctgctcctc gggtcgtccg cgccggccca tcgcgatggc
     5701 ctgacgatcg cggtcggggt cctcgccggt gtaggtccgc gaggcggccg tctccgtcac
     5761 tcccggcgcg acggcgttga cgcggatgtt gtccggcgcc agctcgacgg ccatcgtgcg
     5821 agtcatcgcc gccaccgcgg ccttggccgt tccgtaggcg atgtggaatg gcgcggtgtt
     5881 catgccgctg atcgaagaaa ttgagacgat cgacccgggg tcgccgtgct gccggatttc
     5941 gcgggccacc gcctggctca tgaagaacgc cgtctccagg ttgcgggtga acagggcgcg
     6001 ccagtcgtcg cgcgtcaccc gggtggaggg catccacgtc gacgggtccg caccgcccgc
     6061 cacgttgacg agcccgtaca gcttgccgtc ggtgcgccgg acatgctcga tcacggtcgc
     6121 gatgccgtcg tcggtcgacg cgtcggccgc caccggcacg acggacagcc cccgctgcgc
     6181 gagcggggac acgtgctcgt ccaggttttc cttcgaccgg ctgaccgcga cgacagtcgc
     6241 gccggcctcg gcgaccagtc gcgtcaccgt ggtcccgatg ccacccccgc cagcgcccga
     6301 caccacgacg atgcggccat ccagcttcaa gagatcgtcc atggtcacgt catttgtccg
     6361 gacaacatac ggtgctctgc atattgcaga acactattcc gcagcgggaa tgtccccgtc
     6421 aaggtcggct tagcagcggg ctgacactat tgtctggact aaagaggctt gataccgtcc
     6481 gaggtcatga cctcggtgct gcaggcccgc tactccgccg ccaacccgac cctcgccgac
     6541 ggctggcggc tcgagcggct gaccccgccc agccggctgt tcggtgccaa cggtctgcgg
     6601 accggtccgg acggacgcgt gtacgtcgcg caagtgacag gcagtcagat cagcgcactg
     6661 gatctcaaga ccggccagct ggacaccgtg agcgcgaagg gcggcgacat catcgcgccc
     6721 gacgacgttg ccttcgatcc gcgcggcaac ctgtacgcga cggaagtgat ggacggccgg
     6781 gtcagtgtgc gcgagcccgg cggcacgacc cgcgtgttgc gcgacgacct gccctgcgcc
     6841 aacggaatca ccgtgcatgc gggccggctg ttcatcaacg agtgccgcga cggcggccgg
     6901 ctcatggagc tcgacggcag cggatccatc gtccgaatgc tggccgagaa cctgccgtcg
     6961 cccaacgcga tggaggtcgg tcccgacggg atgctctatt acccgctgat gacggcgaac
     7021 gagatctggc gtatcgaccc gaatggcggt gagccacaac gggttgccgg tgatctcggg
     7081 gtgcccgacg cggtgaagtt cgattccgag ggcttcatcg tgtcga
//
LOCUS       DASRJA010000759        13779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4085385,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000759 DASRJA010000000
VERSION     DASRJA010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13779
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4085385"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /gene="hemC"
                     /locus_tag="VFC01_34205"
     CDS             complement(<1..714)
                     /gene="hemC"
                     /locus_tag="VFC01_34205"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZN84712.1"
                     /translation="MTDSHEVIRIGTRGSLLAKTQASVIKDALAANGHAAELVIISTE
                     GDRSTGPIANIGVGVFTAALREAIDERRVDMAVHSYKDLPTAPDPRFNIAAIPPREDP
                     RDALVARDGMVLGELPVGSVIGTSSPRRTAQLRALGLGLEIRPLRGNLDTRLNRVSNG
                     DLDGIVVARAGLARIGRLADVTETLEPVQMLPAPAQGALAVECRAGDTELAALLAELD
                     DADTRAAVTAERVLLAELEA"
     gene            complement(718..2055)
                     /locus_tag="VFC01_34210"
     CDS             complement(718..2055)
                     /locus_tag="VFC01_34210"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZN84713.1"
                     /translation="MSVLLFGVSHRSAPVSVLEQLSTDEDDQAKIVDQVLQSSLVTEA
                     MVLSTCNRVEVYAVVEAFHGGLSVIGSVLSEHSGMSLGDLTKYAYVRYAEAAVEHLFA
                     VTSGLDSAVIGEQQVLGQVRRAYAAAEANHTVGRTLHELSQRALAVGKRVHSETGIDS
                     AGASVVSVALGMAETKLGGLAGRSAVVVGAGSMGALSAKHLARAGVERVHVVNRTLPR
                     AKRLAHNIRELGVAADAFPFDHLPPVLTDADVVVCCTGAVRPVVSLADVHHALARREH
                     QLVMCDLGMPRDIDPAVAGLPGVFVVDMDRIQREPSARAAASDADAAREIVAAEVANY
                     LAGQRMAEVTPTVTALRQRAADVVEAELLRLDNRLPGLDADHREEVAKTVRRVVDKLL
                     HAPTVRVKQLASAPGGDSYAEALRELFELDQQAVDAVAASELPLVTKDLDKTE"
     gene            complement(2121..2378)
                     /locus_tag="VFC01_34215"
     CDS             complement(2121..2378)
                     /locus_tag="VFC01_34215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZN84714.1"
                     /translation="MNRRVELLTREGCTICSGAVTQLRRLADELGFELRITDVDAAAA
                     AGDSALRAEFGDRLPVVLLDGAEHSYWEVDEPRLRADLANR"
     gene            2417..3298
                     /locus_tag="VFC01_34220"
     CDS             2417..3298
                     /locus_tag="VFC01_34220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HZN84715.1"
                     /translation="MSETGDVVDQELAGEASAEAAVTELAVEAVTAPPSPPPDLTAAA
                     FFDVDNTLVHGSSLVHFARGLAARKYFTYGDMGRFIYAQAKFQLLGRENSDDVAAGKT
                     KALAFIEGRSTAELEAVGEEIYDEIIADKIWPGTRALAQMHLDAGQQVWLVTATPYEL
                     AATIAKRLGLTGALGTVAESVDGVFTGRLVGDILHGTGKAHAVRSLAIREGLNLRRCT
                     AYSDSFNDVPMLSLVGTAVAINPDNDLRALARERGWEIRDFRTARKAARIGVPSAVAL
                     GAVGGALAAIVSRRQDS"
     gene            3351..3890
                     /locus_tag="VFC01_34225"
     CDS             3351..3890
                     /locus_tag="VFC01_34225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HZN84716.1"
                     /translation="MAIAEDIIGTHYRYPDYFEVDREKIREFARAVKDDHPAHYSEEA
                     AKECGYDALIASLTFLAVAGRKVQSEIFNQFDVPINMERVLHRDQKIVFHRPILAGDK
                     LYFDSYLDSVTESHGAVVTEIRGEVTDADGNPVLTSIVTVMGEAASDTEADEVSAQIA
                     AARDAAIAKMIAGQSSKKS"
     gene            complement(3894..4991)
                     /locus_tag="VFC01_34230"
     CDS             complement(3894..4991)
                     /locus_tag="VFC01_34230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN84717.1"
                     /translation="MGWGEGTNVAGEPKAKVIPLHSNSGRTSAGRRAAAAARAESSRR
                     HPSLLSDSGGRATAEQIAAVVREIDQRRGSVSGTATVEETPSELAKRVASIAEFVRKR
                     MLGDYTVDEFGFDRHLNDAIFLPLLRVFFNSWFRVEVSGIENLPKSGAALVVANHAGV
                     LPFDGLMTAVAVHDKHPTHRDLRLLAADMVFDMPMIGQAARKAGHTMACTADAHRLLA
                     AGELTAVFPEGYKGLGKHFKDRYKLQRFGRGGFVGAALRTKAPIVPCSIVGSEEIYPM
                     IADVKLLARLFGLPYFPVTPLFPLAGPVGLVPLPSKWHIQFGEPISTADYDESAADDP
                     MITFELTDQVRETIQQTLYQLLANRRNMFLG"
     gene            complement(5008..6096)
                     /locus_tag="VFC01_34235"
     CDS             complement(5008..6096)
                     /locus_tag="VFC01_34235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN84718.1"
                     /translation="MDSGGKSNGSDSRDTVHYPKVVLVTGACRFLGGYLTARLAQNPL
                     INHVIAVDAIAPSKDLLRRMGRAEFVRADIRNPFIAKVIRNGDVDTVVHAAAASYAPR
                     SGGRATLKELNVMGAIQLFAACQKAPSVRRVVLKSTSEVYGSSSRDPVLFTESSSRRR
                     PPGEGFARDSIDIEGYARGLGRRRPDIAVSILRLANMIGPAMDTALSRYLANPVVPTV
                     VGHDARLQLLHEQDALGALERATMAGRAGTFNVGASGVIMMSQAIRRSGRIPLPVPRS
                     ALWAVDSLRRATRYTELDREQLDYLSYGRVMDTTRMRNDLGYSPKWTTAEAFDDYVRG
                     RGLSPIIEPRWVRSMESRAVSAAQRWGR"
     gene            complement(6126..6227)
                     /locus_tag="VFC01_34240"
     CDS             complement(6126..6227)
                     /locus_tag="VFC01_34240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HZN84719.1"
                     /translation="MGSVIKKRRKRMSKKKHRKLLRRTRVQRRKLGK"
     gene            complement(6313..6576)
                     /locus_tag="VFC01_34245"
     CDS             complement(6313..6576)
                     /locus_tag="VFC01_34245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN84720.1"
                     /translation="MTSMNGPSARDSASGKAARDASPTDGQPPRAQFLTVAEVASLMR
                     VSKMTVYRLVHNGELPAVRVGRSFRVHAKAVHDMLESSYFDAG"
     gene            complement(6716..7588)
                     /gene="proC"
                     /locus_tag="VFC01_34250"
     CDS             complement(6716..7588)
                     /gene="proC"
                     /locus_tag="VFC01_34250"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZN84721.1"
                     /translation="MARIAIVGGGSIGEALLSGLLRAGRQVKDLVVAEKHPDRAKYLS
                     NTYSVLVTTVADAVDTATYVVVAVKPSDAGTVVNEIAEAAAKAESEAAEQVFVSVVAG
                     VTTEFYENKLPAGSPVIRVMPNAPVVVGGGVSALARGRFATAEHLKEVSAIFDAVGGV
                     LTVPESQLDAVTAVSGSGPAYFFLMVEALVDAGVAAGLTRSVATDLVVQTMAGSAAML
                     LERLDEAQPAGDAAMGTAMDTTAAQLRATVTSPGGTTAAGLRELERGGLRAAVANAVE
                     SAKTRSEQLGITSE"
     gene            complement(7642..8475)
                     /locus_tag="VFC01_34255"
     CDS             complement(7642..8475)
                     /locus_tag="VFC01_34255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZN84722.1"
                     /translation="MPGSTLFTDAMALTPAGDGVYHGVLNEHWTIGPKVHGGAMLALC
                     ANAARTEHWAHARSAGEEDVQPIALSGNFLWAPDPGPMRVVTTVRKRGRRVSLIDVEL
                     NQGDRTAVRAAITLGDPEHHVPPLLSVNPVIALMQPEPPPGLEPIGPGHPMEDIVHLA
                     HGCDIRPSLTTFTPRTDGGPPIIEYWVRPKGVAPDVLFALLCGDVSAPVTFGVNRTGW
                     APTVQLTAYLRALPADGWLRVLCTTVQIGQDWFDEDHIVVDCEGRIIVQSRQLAMVPD
                     A"
     gene            complement(8493..9338)
                     /locus_tag="VFC01_34260"
     CDS             complement(8493..9338)
                     /locus_tag="VFC01_34260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZN84723.1"
                     /translation="MRPAIKVGLSTASVYPLRTEAAFEYAARLGYDGVELMVWAETVS
                     QDVDAIEKLSEQYDMPVLSVHAPCLLISQRVWGANPIPKLERSVRAAEQLGAQTVVVH
                     PPFRWQRRYAEGFSQQVADLEASSDVMVAVENMFPFRADRFFGAGQTSIERMRKRGGR
                     PGPGISAFAPSYDPLDGNHAHYTLDLSHTATAGTDAVDMARRMGDGLVHLHLCDGSGA
                     STDEHLVPGRGTQPTVEVCEMLAAGTFSGHVILEVTTSGARTATEREGLLKESLQFAR
                     QHLLR"
     gene            complement(9354..10361)
                     /locus_tag="VFC01_34265"
                     /pseudo
     CDS             complement(9354..10361)
                     /locus_tag="VFC01_34265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10426..11391)
                     /locus_tag="VFC01_34270"
     CDS             complement(10426..11391)
                     /locus_tag="VFC01_34270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HZN84724.1"
                     /translation="MRLGVLDVGSNTVHLLVVDARRGGHPTPMSSTKASLRLAEAIDG
                     SGKLTRKGADKLISTIDEFAKIAASSGCAELMAFATSAVRDAKNSEDVLTRVLAETGV
                     ALQVLSGADESRLTFLAVRRWYGWSAGRIINIDIGGGSLELSNGVDEEPDVALSLPLG
                     AGRLTREWLPDDPPGRRRVAMLRDWLATEIADTGAKVLKAGNPDLAVATSKTCRSLAR
                     LTGAAPSGAGPRVKRTLTATGLRQLINFISRMTAADRAELEGVSAERAPQIVAGALVA
                     EASMKALDIESVDICPWALREGLILRKLDSEADGTALVETSVRGR"
     gene            11404..12201
                     /locus_tag="VFC01_34275"
     CDS             11404..12201
                     /locus_tag="VFC01_34275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84725.1"
                     /translation="MGLPLNAVTASHPGEVELDFAREWVEFYDPDNPEHLIAADLTWL
                     LSRWTCVFGTPACKGTVEDRPDDGCCSHGAFLSDDDDRARLDDAVKQLTDEDWQFREK
                     GLGKKGYLEWDEYDDKPNLRTRKYKGACIFLNRPGFPGGIGCALHSKALKIGVEPLTM
                     KPDVCWQLPIRRTQEWVERPDGSEILKTWITEYDRRGWGEGGADLHWYCTGDPNAHVG
                     AKQVWESYAPELTELLGEKAYAELAGICKRRSGLGLIAVHPATRLAE"
     gene            12198..12491
                     /locus_tag="VFC01_34280"
     CDS             12198..12491
                     /locus_tag="VFC01_34280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZN84726.1"
                     /translation="MTLFLFKLIPPRADFAQTMTADEQQAMAEHMEYWQQLLKDGRVV
                     VYGPVADPEGVWGMGVLRAADRGEVLAIGERDPSVLAGVNTFEVFEIMGGQTG"
     gene            complement(12429..13703)
                     /locus_tag="VFC01_34285"
     CDS             complement(12429..13703)
                     /locus_tag="VFC01_34285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HZN84727.1"
                     /translation="MTTQTTQHIDHTRNISPWVRGGGPAVAAAALLTYVAVYLQWPTL
                     KLQIDAMVYRFAAERLLAGLDLYSTGFTGKPNELLFIYPPFAAICAVPLALLDRSSVQ
                     VLWLLGLVVALTYAVVKMLRATGLPAGRGLFSLAALLVGVIAWLEPMRLTAELGQINV
                     LLLVLVVADLLKQSRWSGVGIGLAAGIKLTPALFIIYLLVTRRIRAALVAMGTFAATV
                     GLGFAVAPGDSVTFWLRGRFDDVNRISHDPLANTSVRGLFLRLHWSPTLATITAIAVA
                     AVAVAIAAIAYRRGQAVLAVGIVGMASAAASPFSWSHHWVWFAPLVVHLGYRAYVLGS
                     RWAAAAMWSVCAVVGGWFVAVVGDTPQAGILSLRPGGTWDEILPGAYVFVFILVLPAT
                     AVWLWRLRAERHLTRLPTHDLEHLEGVDAGQN"
BASE COUNT         2378 a         4682 c         4467 g         2252 t
ORIGIN      
        1 cgcctccagt tcggcgagca ggactcgttc agcggtgact gcggcgcgcg tgtcggcgtc
       61 gtccaactcc gccagcagcg cggccagctc tgtgtcgccc gcacggcact cgaccgcgag
      121 ggcgccttga gccggcgccg gcaacatctg caccggctcg agagtctcgg tgacatccgc
      181 cagccgcccg atgcgggcca gtcccgcccg tgcgaccacg atgccgtcga gatcaccgtt
      241 gctaaccctg ttcaacctgg tatcaaggtt gcctcgtagg gggcggattt ccaaaccgag
      301 acccaatgct ctaagctgcg cggtccgtcg cgggctcgac gtccctatta ccgagcccac
      361 cggcaactct ccgagcacca ttccgtcgcg ggccacaagc gcgtcgcggg ggtcctctcg
      421 cgggggtatc gcggcgatgt tgaacctcgg gtcgggtgca gtcggcaaat ctttgtagga
      481 gtgcacggcc atgtccacgc ggcgttcgtc aatggcctcg cgcagcgccg cggtgaacac
      541 acccacaccg atattggcaa tcggtccggt cgaccggtcg ccttcggtgg agatgatcac
      601 caattcggcg gcgtgcccgt tggcggcgag ggcgtctttg atgacgctgg cctgcgtctt
      661 cgccaacagg ctgccccggg tgcctatccg gattacttcg tggctatcag tcaagcgcta
      721 ctcagtcttg tcgagatctt ttgtcaccaa aggcaattcg cttgcggcga cggcgtctac
      781 ggcctgctgg tcgagctcga acaactcgcg cagagcctcg gcgtagctgt cgccgccagg
      841 tgcgctggcc aactgtttga cccgcaccgt cggcgcgtgc agaagcttgt cgacgacgcg
      901 gcggacggtc ttggcgactt cctcgcgatg gtcggcgtcg agccctggca gccgattgtc
      961 cagccgcagc aactcagcct cgaccacgtc ggcggcgcgc tgccgcagcg cggtcactgt
     1021 cggggtgacc tccgccattc gctgtccggc caggtaattg gcaacctcgg cagcgacgat
     1081 ctcgcgggcg gcatcggcgt cggaggcagc tgcgcgggcc gacggttcgc gctgaatgcg
     1141 gtccatgtcg acgacgaaga cgcccgggag accggcgacc gcgggatcga tgtcacgcgg
     1201 cattccgagg tcgcacatga ccagctggtg ctcgcggcgg gccagcgcgt gatggacgtc
     1261 ggccaacgac accaccggcc gcaccgcacc cgtacagcac acgacgacgt cagcatcggt
     1321 cagcacgggc ggcaggtgat cgaacgggaa ggcgtcggcg gcgacgccga gttcacggat
     1381 gttgtgggcg agccgcttcg cgcgcggcag cgtcctgttc acgacgtgga cgcgctcgac
     1441 ccccgcgcgc gccaaatgct tggccgatag cgcccccatc gagccggcgc cgacgacaac
     1501 ggcgctgcgg ccggccaggc caccgagttt cgtttcggcc atgccgagcg ccaccgacac
     1561 caccgatgcc cctgcggagt cgatgcctgt ttcggagtgc acccgcttgc ctaccgccag
     1621 ggcacgctgg gacagctcgt gcagggtgcg cccgacggtg tggttggctt cggcggcggc
     1681 atatgcacgc cggacctggc cgagcacctg ctgctcgccg ataactgcgg agtccaggcc
     1741 gctggtcacg gcgaagagat gctcgacggc agcttcggcg taccgcacgt aggcgtactt
     1801 cgtcaggtcg cccagcgaca ttccggagtg ttcggagagc actgagccga tcaccgacaa
     1861 gccgccgtgg aacgcctcga caacggcgta gacctcgacg cggttgcagg tcgacagcac
     1921 catggcctcg gtcaccaacg aggactgcag cacctggtcg acgatcttgg cctgatcgtc
     1981 ctcgtcggtg ctcaactgct ccagcaccga caccggcgcg ctgcgatgcg aaaccccgaa
     2041 aagcaagacg ctcacggctt gtccaccacg tcatccacgg taatcgttga ccagctgggc
     2101 taccaaattt gctcgcgtct ttatcggttc gccagatccg cccgcaaccg gggctcatcg
     2161 acctcccagt aactgtgctc ggcgccgtcc aacagcacca ccgggagccg gtcaccgaac
     2221 tcggcgcgca gcgcggaatc gcctgccgcc gcggcggcgt cgacgtccgt gatcctcagt
     2281 tcgaagccca actcatcggc caaccgccgc agctgcgtca ccgcccccga gcagatcgtg
     2341 cagccctccc gtgtgagcaa ttccacccgc cggttcaccc cgccagtatg gcgagccggt
     2401 gtcctactgt taattcgtgt ccgaaaccgg cgacgtcgtc gaccaagagc tcgcgggcga
     2461 ggccagtgcg gaggccgccg tcaccgagct tgcggtcgag gccgtcaccg ccccgccatc
     2521 cccaccgcca gacctgaccg ccgcggcgtt cttcgacgtc gacaacacgc tggtgcacgg
     2581 ctcgtcgctg gtgcatttcg cccgcggcct ggccgctcgc aagtacttca cctacggcga
     2641 tatgggccgc ttcatctacg cgcaggccaa gtttcagctt ctcggccggg agaacagcga
     2701 cgacgtggcc gccggaaaga ccaaggcgct ggcgttcatc gagggccggt cgactgccga
     2761 actggaggcg gtcggcgagg agatctacga cgagatcatc gccgacaaga tctggcctgg
     2821 tacccgcgcg ctggcccaga tgcacctcga cgcgggccag caggtgtggc tggtcaccgc
     2881 gacgccctac gagttggccg cgacgatcgc caagcggcta gggctgaccg gcgcactggg
     2941 caccgtcgcc gaatcggtgg acggggtgtt caccggccgg ctggttggcg acatcctgca
     3001 cggcaccggc aaggcgcacg cagtgcggtc gctcgccata cgcgaggggc tgaacctgcg
     3061 gcgctgcacg gcatactccg acagcttcaa cgacgtgccg atgctgtcgc tggtcggcac
     3121 ggccgtcgcg atcaaccccg acaacgatct gcgcgccctg gcccgcgagc ggggctggga
     3181 aatccgtgat ttccgcaccg cacgcaaggc cgcccggatc ggcgtgccgt cagcggtggc
     3241 gctcggtgcc gtcggcgggg cgttggcggc gattgtgtcg cgacgccaag acagctgaaa
     3301 ggccgctgat aggctcgccc gctaacacct agaggggaag aacgaccagc atggccattg
     3361 ctgaagacat catcggaacc cactaccggt accccgacta cttcgaggtc gaccgggaga
     3421 agatccgtga gttcgcgcgc gccgtcaagg acgaccatcc ggcgcactac agcgaggaag
     3481 ccgcgaagga gtgcggctac gacgcgctga tcgcctccct gacgttcctg gcggtcgccg
     3541 gccgcaaggt ccagtcggag atcttcaacc agttcgacgt gccgatcaac atggagcggg
     3601 tgcttcaccg cgaccagaag atcgtcttcc atcgcccgat cctggccggg gacaagctgt
     3661 acttcgactc ctatctcgat tcggtgaccg agtcgcatgg cgccgtggtc accgagatcc
     3721 gcggcgaggt gaccgacgcc gacggcaacc cggtgctaac cagcatcgtc acggtgatgg
     3781 gcgaggccgc ctccgatacc gaggccgacg aagtgagcgc acagatcgcc gccgcacgcg
     3841 acgctgcgat cgcgaaaatg attgctgggc aaagctcgaa gaagagctag acctcagccg
     3901 aggaacatgt tgcggcgatt cgccaacagc tgatacagcg tctgctgaat ggtttcgcga
     3961 acctggtcgg tcagctcgaa cgtgatcatc ggatcgtcgg cggccgattc gtcgtagtcg
     4021 gcggtcgaaa tcggctcgcc gaactggatg tgccacttcg acggaagcgg caccaacccg
     4081 accggccctg ccagcgggaa cagcggggtg accgggaaat acggcagtcc gaacagccgg
     4141 gccagcagct tgacgtcggc gatcatcggg tagatctcct cggacccgac gatcgagcac
     4201 ggcacgatcg gcgccttggt tcgcagcgcc gctccgacga agccaccgcg gccgaagcgc
     4261 tgcagcttgt aacggtcctt gaagtgcttg ccaaggccct tgtagccctc agggaagacg
     4321 gcggtcagct cgccggcggc cagcagccgg tgcgcgtcgg ccgtgcatgc catggtgtgg
     4381 ccggccttgc gcgcggcctg accgatcatc ggcatgtcga acaccatgtc ggcggccagc
     4441 aggcgcaggt cgcggtgcgt gggatgcttg tcgtgcaccg ccaccgcggt catcagcccg
     4501 tcgaacggca gcacgccggc atggtttgcg accaccaacg ccgcacccga cttcggcagg
     4561 ttttcgatcc cgctgacctc aacccggaac cacgagttga agaacacacg cagcaacggc
     4621 aaaaagattg cgtcgttgag atggcgatcg aacccgaact cgtcgacggt gtagtcaccc
     4681 agcatgcgtt tgcggacgaa ctcggcgatc gaggcaaccc gcttggcgag ttctgacggc
     4741 gtttcctcga cggtcgcggt gccgctgacg gagcctctgc gctggtcgat ctcacggacg
     4801 acggcggcga tctgctcggc ggtagcgcgc ccaccgctgt ccgagagcag ggagggatgt
     4861 cggcgcgagc tttcggcacg cgcagcagca gcagcgcgcc gcccagccga agttcggccc
     4921 gaattagagt gcagcggaat gactttggct ttgggctcgc ccgccacgtt ggtaccttct
     4981 ccccacccga ttggaaaata tttcttgtta gcgtccccag cgttgcgccg ccgacacggc
     5041 gcgactctcc attgagcgta cccatcgtgg ctcgatgatc ggagacaatc cgcgacctct
     5101 tacgtaatca tcaaaggcct cggcagtagt ccatttgggg ctgtagccaa ggtcattacg
     5161 catccgcgtc gtgtccatca cccggccgta gctcaaatag tcaagctgct cgcgatccag
     5221 ttcagtgtag cgagttgcgc gcctcagcga atccacagcc cataacgcgg agcgcggcac
     5281 cggcagtgga atccggcccg agcgacggat ggcctggctc atcatgatga cgccggacgc
     5341 gcctacgttg aaggtgccgg ccctgcccgc catggtggcg cgctccagcg cgccgagcgc
     5401 gtcctgctcg tgcagcagct gcagccgtgc gtcgtgaccg acgaccgtcg gcaccaccgg
     5461 gttggccaga taccgcgaca gcgcggtgtc catcgccggg ccgatcatgt tggccagccg
     5521 caggatgctg accgcgatgt ccggtctgcg cctgcccaat ccgcgggcgt agccctcgat
     5581 gtcgatgctg tcgcgggcga agccctcgcc cggcggcctg cggcggctgc tgctctcggt
     5641 gaacagcacc ggatcccgcg aactcgagcc gtacacctcg gaggtggact tgagcacgac
     5701 acgacgcacc gacggcgcct tctgacaggc cgcaaacagc tgaatcgcgc ccatcacgtt
     5761 gagttccttg agcgtggccc gcccacccga gcgcggggca tacgaggccg ccgcggcgtg
     5821 caccacggtg tccacgtcgc cgttgcggat gaccttggcg atgaacgggt tacggatgtc
     5881 ggcgcggacg aactccgcgc gacccatccg tcgcagcaga tccttgctcg gcgcgatcgc
     5941 atcgaccgcg atgacgtggt tgatcaacgg gttctgcgcg agccgagcgg tcagatatcc
     6001 acccaggaat cggcatgccc ccgtgacgag gacgaccttc gggtagtgga cggtgtcgcg
     6061 cgagtcggag ccgttcgatt tgcccccaga atccatctgg acagactagc ggccgagcac
     6121 gaaagctact tgccgagttt tctgcgctgc acccgggtgc gacgaagcag cttgcggtgc
     6181 ttcttcttcg acatgcgctt acgccgcttc ttgatgactg aacccatgaa ctccgctacc
     6241 taatgtttcc aagcctcttg acccgcacac cttacctggg cggcgtaggt aaacggaaaa
     6301 cgcacagccc cgctagccag cgtcgaagta tgagctttcc aacatgtcgt ggacagcctt
     6361 ggcgtgcacc cggaacgagc gtccgacgcg aacggcgggc aactcgccgt tgtgcaccaa
     6421 ccggtacacg gtcatcttgc tgacccgcat caggctcgcc acctccgcga cggtgagaaa
     6481 ttgagctcgt ggcggctggc cgtcggtcgg gctggcgtca cgcgccgcct tcccactcgc
     6541 cgagtcccgc gccgatggcc cgttcataga cgtcatcgca acccaatcca tcaggcacgg
     6601 gcagttccag cggcttcccc accgctggca ccaacccgtg cttaccactg ggagaatagc
     6661 gtggcggatg gggttactgc gacgggtgtg ggctaatcgg tccgaaattg attaattact
     6721 cggatgtaat tccgagctgc tcagagcgcg tttttgcaga ttcaacggca ttggcgaccg
     6781 ccgcccgcaa accgccccgt tcgagttcgc gcagcccagc agcggtagta ccgcccggcg
     6841 aggtaacagt ggcccgcaat tgcgcggcgg tggtgtccat cgcggtgccc atcgccgcgt
     6901 cgccggccgg ctgagcctcg tccagccgct ccagcaacat cgcggccgag cctgccatcg
     6961 tttgcacgac cagatcggtc gccaccgacc gagtcagccc cgccgcgacg cccgcgtcga
     7021 cgagcgcctc caccatcagg aagaagtacg ccggcccgga gcccgacacc gcggtgaccg
     7081 cgtcgagttg cgactccggc accgtcagca caccgcccac cgcatcgaag atcgccgaca
     7141 cctccttgag gtgctcggcg gtggcgaacc gaccgcgcgc aagcgcgctg actcccccgc
     7201 cgacgacgac gggcgcattg ggcatcaccc ggatcaccgg cgacccggcc ggcagcttgt
     7261 tctcgtagaa ctcggtcgtc acacccgcga cgaccgacac gaaaacctgt tcggcggctt
     7321 cactttcggc ctttgccgct gcttcggcga tctcgttgac gacggtcccg gcatcggagg
     7381 gcttgacggc gacgaccaca taggtcgcgg tgtcgaccgc gtcggcgacc gtcgtcacca
     7441 acaccgaata cgtgttggac agatactttg cgcggtcagg atgcttctcg gcgaccacca
     7501 ggtccttgac ctgccgcccg gctcgcagca gcccggatag cagtgcctcg ccaatacttc
     7561 caccgccgac gatcgcgatt ctggccatgc gcgcaagcat tgcatgcgcg ctgcggcact
     7621 ggttattcga cgcctgcggc gtcaggcatc gggcaccatc gccagttggc ggctctgcac
     7681 gatgatgcgg ccctcgcagt cgaccacgat gtggtcctcg tcgaaccagt cctggccgat
     7741 ctgcaccgtc gtgcacaaga cgcgcagcca gccgtcggct ggcaacgccc gcagatacgc
     7801 ggtgagttga accgtcggcg cccaccccgt ccggttcacc ccgaacgtca ccggcgcgga
     7861 cacgtcgccg cacagcagcg cgaaaagcac gtccggcgca acgcctttcg ggcgcaccca
     7921 gtattcgatg atcggtggcc cgccgtccgt ccgcggcgtg aatgtcgtca gcgacggccg
     7981 gatatcgcag ccgtgcgcca gatgcacgat gtcttccatc ggatgcccgg gaccgatcgg
     8041 ttcaaggcca ggcggcggct cgggctgcat cagtgcgatg acggggttga ccgacaacag
     8101 cggcggcaca tgatgctcgg ggtcgcccag cgtgatggcc gcgcggacgg ctgtgcggtc
     8161 gccctgattc agttcgacat cgatgaggct gacgcgacgg ccccgcttgc gcacggtcgt
     8221 gaccacccgc atcgggccgg gatcgggggc ccacaggaag ttgcccgaca gcgctatcgg
     8281 ctggacgtct tcctcccctg cgctgcgcgc gtgggcccag tgctctgtgc gggcggcgtt
     8341 ggcgcacagc gccagcatcg ccccgccatg cactttcggc ccgatggtcc aatgctcgtt
     8401 cagcacaccg tgatacacac cgtccccggc tggggtgagc gccatggcgt cggtgaacag
     8461 ggtggatccc ggcatctgat gtcctttgtg cattagcgca gcaggtgctg gcgggcgaac
     8521 tgcaacgact ccttcagcag cccttcgcgt tcggtggcgg tgcgtgcgcc cgatgtggtc
     8581 acctcgagga tcacatgccc ggaaaaggtg ccggcagcga gcatctcgca gacctccacg
     8641 gtgggctgag tgccgcggcc cggcaccagg tgctcgtcgg tcgaggcgcc gctgccgtcg
     8701 cacaggtgca agtgcacgag cccgtcgccc atccggcgcg ccatgtcgac cgcgtcggtg
     8761 cctgccgtcg cggtgtgcga caggtcgagt gtgtagtggg cgtggttgcc gtcgagcggg
     8821 tcatacgacg gcgcgaacgc cgaaatgccc ggaccgggcc tgccgccgcg cttgcgcatc
     8881 cgctcgatcg acgtctgccc cgcgccgaag aatcggtccg cgcgaaacgg aaacatgttc
     8941 tccaccgcga ccatcacgtc gctggacgcc tccagatcgg cgacctgctg gctgaagccc
     9001 tcggcgtagc gccgctgcca gcgaaacggc ggatgcacga ccacggtctg cgcgccgagt
     9061 tgttcggcgg cgcgcacgct gcgctcgagc ttcggtatcg ggttggcccc ccacacccgc
     9121 tgggagatca gcagacacgg cgcgtgcacc gacagcacag gcatgtcgta ctgctcggag
     9181 agcttttcga tggcgtcgac gtcctggctg acggtttccg cccacaccat cagctcgacg
     9241 ccgtcgtagc ccagccgcgc cgcgtactcg aaggcggcct cggtcctcag cggatagacc
     9301 gaggcggtcg acagaccgac cttgatggca gggcgcactg tgtctgtagg tgtctagccc
     9361 gattgcagca gtgccaacgg tcccaacgtg accagcgccc cgaccgccac cgcgatcagc
     9421 gtgctgccga tgtcctcggt cttgcgcacc acgcgcacac ccaccaccag gccgaggatc
     9481 accagcaccg acagcaccag ggccacgatg ttgttccact tccacagctg gtcgaacgcg
     9541 atgaacaggc cggcgccgaa cgcgaccgcg atgatgcact ggccgacgac ccaggcgccg
     9601 cgcaggaacg acgatgtcga agtcgacgaa ggcaccgcct ccgtgtccgc atcatcgtcg
     9661 aggtcgatct cgtcgggtcg gggcggcgcg gcgcgccgag ccaggtcatc agcgacggtc
     9721 tgaccgccga acagcgtgcc cgcggaggac cgcaagtacg acggcagttc ctcgttccta
     9781 tcctcgaccg cggcctcggt gtcctcgtcc tcgatctccg gctcgtccgc caaatcgagc
     9841 ggggcggcga acaggtcctc ctcgtcgacc gggtcggggc tcatctgctc ggcgcccctg
     9901 cccaccggcc ccacacgtcg cggggcgggg gtgtaccgcg gtcgacgcgg cggcagcgcg
     9961 aagaagtcga ccggttcgtc atcggtgtcg cgctgatcga tatgggccgc gtagtcggcg
    10021 accgcgtcgg cgtagtcgtc gtcgacgtcg tcgtattcgt cctcggccgg ttccgcctcg
    10081 gcttcaacgt cgtcgacgta gccgcggaca ccattggtcg aaaccgcctg gggctcgtcg
    10141 gtgactgccg ggatcgtccg ggtggtttcg gtgtcgtcga cgtcatcgtc gccgacgatc
    10201 gggatttcgc cggtcaactc ggcgaccgtg acggcttagt tgttgccgcg gcgcctgcgg
    10261 cgccggccgc cgacgggcgg ggcaccgatc gtgccgttct tcgccagcaa ctccgcgacc
    10321 gagatcggtc gcgagccgct gtggtcttct ggtcctgtca tcctgtgttg cctctgggcc
    10381 tagctaatgt ccaagtcggc gccattgcgc ctccgtccgc tcgtttcagc gtccccgcac
    10441 ggacgtttcg accaaggccg tgccgtcggc ttcgctgtcg agtttccgca gaatcagccc
    10501 ttctcgcaac gcccagggac agatatccac actttcgata tcgagagcct tcatgctcgc
    10561 ctcagctacc aatgcgccgg ccacgatctg tggcgcccgc tcggcactca ccccttccaa
    10621 ctccgcacgg tcagccgcgg tcatcctaga gatgaaattt atgagctgcc tgaggccggt
    10681 cgctgtgagc gtcctcttca cccgtggtcc agcccccgac ggggccgcgc cggtgagtcg
    10741 ggccagcgag cggcaggtct tcgacgtcgc gaccgccagg tcggggtttc ctgccttgag
    10801 caccttggcg ccggtgtcag cgatctcggt ggccagccag tcgcgcagca tcgccacccg
    10861 gcgccggccg ggcggatcgt cgggcagcca ttcgcgggtg agccggcccg cgcccagcgg
    10921 cagcgacagc gccacatcgg gttcctcatc gacgccgttg gacagctcca gcgaaccgcc
    10981 gccgatgtcg atgttgatga tgcgtcccgc gctccagccg taccaccttc gcaccgccag
    11041 gaacgtcaac cgtgactcgt cggccccgct gagcacctgc agcgccacac cggtctcggc
    11101 gagcacccgg gtgagcacgt cctcagaatt cttcgcgtcg cgcacggcgg acgtggcgaa
    11161 tgccatcagc tcggcacagc cggagctggc ggcgatcttg gcgaactcgt cgatggtgct
    11221 gatgagtttg tcggcgccct tgcgggtcag cttgcccgag ccgtcgatcg cctcggcgag
    11281 ccgcagcgag gccttggtgg aactcatcgg agtcgggtgg ccgccgcggc gggcgtccac
    11341 caccaacaga tggaccgtat tgctgccgac atccagcacg cctaatcgca cgcacccaac
    11401 ttaatgggtt taccgttgaa tgctgtgacg gcatcacatc ccggcgaggt cgaactggac
    11461 tttgcccgcg aatgggtgga gttctacgac cccgacaacc ccgagcattt gatcgcggcg
    11521 gacctgacgt ggctgctgtc gcggtggacg tgcgtgttcg gcacgcccgc gtgcaagggg
    11581 acggtggagg atcggcccga cgacgggtgt tgctcgcacg gtgcgtttct gtccgacgac
    11641 gacgaccgtg cccggctgga tgacgccgtc aagcagctga ccgacgagga ttggcagttc
    11701 cgcgagaagg ggctcggcaa gaagggctac ctggagtggg acgagtacga cgacaaaccc
    11761 aaccttcgta cacgaaagta caagggcgcc tgcatatttc tgaaccggcc cgggttcccc
    11821 ggcgggatcg gctgcgcact gcacagcaag gcactcaaga ttggtgtcga gccgttgacg
    11881 atgaagccgg atgtgtgctg gcagttgccg atccgccgca cccaggagtg ggtggagcgg
    11941 ccggatgggt cggagatcct caagacgtgg atcaccgagt acgaccgccg cggctggggt
    12001 gagggcggtg ccgatctgca ttggtactgc accggcgacc ccaatgcgca cgtcggcgcc
    12061 aagcaggtgt gggagtcgta cgcccccgaa ctgaccgagc tgctcggtga gaaggcatac
    12121 gccgagctgg ccggaatatg taagcggcgc agtggtttag gactgatcgc tgtgcatccc
    12181 gcgacgcgcc tggccgaatg accctctttt tgttcaagct gattccgccg cgcgcggatt
    12241 tcgcgcagac gatgactgcg gacgagcagc aggcgatggc cgagcatatg gagtactggc
    12301 agcagctgct caaggacggt cgcgtggtgg tctacggtcc ggtcgccgac cccgaaggcg
    12361 tgtggggcat gggcgtgttg cgcgccgccg accggggcga ggtactcgcg atcggcgagc
    12421 gtgatccgtc agttctggcc ggcgtcaaca ccttcgaggt gttcgagatc atgggtgggc
    12481 agacgggtta ggtgcctttc ggcgcgcaac cgccacagcc acaccgccgt cgccggcaag
    12541 acgaggatga acacgaaaac ataagcgccg ggcaggattt cgtcccacgt accaccgggt
    12601 cgcaatgaca gtatgcccgc ctgaggcgtg tcgccgacga cggccacgaa ccagccgccg
    12661 acgacagcgc agaccgacca catcgccgcg gcggcccagc ggctgccgag cacatacgcg
    12721 cggtacccca agtgaacgac caacggcgcg aaccacaccc agtggtggct ccagctgaac
    12781 ggtgaagcgg ctgccgatgc catgccgaca atgccgacgg ccaatacagc ctgaccacgt
    12841 cgatacgcaa tcgcagcgat cgccaccgcg acggcggcga cggcaatcgc ggtgatcgtg
    12901 gccagcgtcg gcgaccaatg caggcgcaga aacaaaccgc gcacactggt gttcgccagc
    12961 ggatcatgcg agatccggtt gacgtcgtcg aagcggccgc gcaaccagaa ggtgacggaa
    13021 tcgcctggag caactgcgaa tccgagtcca acggtggccg cgaacgtgcc catggccacc
    13081 agggccgccc ggatccgacg ggtcaccagg agatagatga tgaacaacgc cggggtcagt
    13141 ttgatgccgg cggccagccc gatcccgaca cccgaccagc ggctctgctt caggagatcg
    13201 gccacgacga gcacaagcag aaggacgttg atctgcccca gctcggcggt cagccgcatc
    13261 ggctcgagcc atgcgatcac accgaccagg agcgcagcga ggctgaagag gccgcgaccg
    13321 gcaggcaggc ccgtcgcccg cagcatcttc accaccgcgt acgtcagcgc gacgaccaag
    13381 ccaagcagcc acagcacctg caccgaggaa cggtcgagca aggcgagcgg gaccgcgcag
    13441 atcgccgcaa atggtggata gatgaacaac aactcgttgg gcttgccggt gaaacccgtc
    13501 gagtaaaggt caagccccgc cagcagtcgc tcggcggcga agcggtacac catcgcgtcg
    13561 atctgcagct tcagcgtcgg ccactgcaga tataccgcga cgtaggtcag cagcgccgct
    13621 gccgcgaccg ctggaccgcc cccgcgaacc caggggctga tgtttctcgt gtggtcaatg
    13681 tgttgtgtgg tctgcgttgt cactcgtcgt cgtccgtcat atcggcccgt aattacgaag
    13741 ggcccacggt agacgagtca gttgtgaagg gctctcagg
//
LOCUS       DASRJA010000760        26573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1807497,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000760 DASRJA010000000
VERSION     DASRJA010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 26573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26573
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807497"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFC01_34290"
     CDS             complement(<1..590)
                     /locus_tag="VFC01_34290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HZN84728.1"
                     /translation="MPILNTARGAIDTSGLGVTLMHEHVFILSPDITANYPEVWGDEA
                     KREDDAIRRLNELKSRGVDSIVDLTVIGMGRYIPRIERVAAATDINIVVATGVYTYND
                     VPMYFHFTGPGGALGDGEPMVDMFVRDIEHGIADTGVKAAILKCATDEPGVTPGVERV
                     LRAVAKAHRLTGVPISTHTHAATRRGLEQQRIFAEEG"
     gene            654..2300
                     /locus_tag="VFC01_34295"
     CDS             654..2300
                     /locus_tag="VFC01_34295"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HZN84729.1"
                     /translation="MPTAAQLMVKCLENEGVSVVFGVPGEENIRFIQALAASPIRYVL
                     TRHEQGAAFMAEMYGRVTGRAAVASATLGPGAINMQLGVADATTNSTPLVAISAQVGQ
                     DREYKESHQYVDLVSMFAPITRWADGVPTSRAIPEMVRKAFKVAETERPAAVYLAVPE
                     HIDADETDYDLTPLPRNVVRAEAPSPGQVQRAVEVLRDAKRPVVLAGHGAARSDATAA
                     LVRFSDELGVQVANTFHGKGVMPDDHPNSIGTLGFMRRDYVNFGFETADVVIAVGYEL
                     QEFDPVRINPNGDKKIIHIHRFPAEVDMHYPVSVGIIGDISESLNALTDALAGHRYDT
                     ADAAPGGGLLAAEFARGQQDSRFPLAPQRIVADTRAALGRSDVVLVDTGATKMWMARL
                     YPTYECNTCLISNGLSTMAFALPGALGVKLAKPDVKVLALVGDGAFLMNSQEIETAVR
                     ERIPLVVLIWEDGGYGLIEWKMDLELGEHHYVSFTNPDVVTYAESFGAKGYRITEADQ
                     LLPTLKKALADDGVSLIACPVDYSENLRLTDTLGQLDETL"
     gene            complement(2305..6432)
                     /locus_tag="VFC01_34300"
                     /pseudo
     CDS             complement(2305..6432)
                     /locus_tag="VFC01_34300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase"
     gene            complement(6467..6640)
                     /locus_tag="VFC01_34305"
     CDS             complement(6467..6640)
                     /locus_tag="VFC01_34305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2613 domain-containing protein"
                     /protein_id="HZN84730.1"
                     /translation="MDRFIVPAAASIVVGLLLGAAAIFGVTLMVQQDSKPPLQAGDPA
                     SSVLNRVEYGDRT"
     gene            6727..6960
                     /locus_tag="VFC01_34310"
     CDS             6727..6960
                     /locus_tag="VFC01_34310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84731.1"
                     /translation="MTTTGRWVFNGLDRAYLDGYEYAVTVAPDQFANLRRVLGADSGA
                     DVVDLVCAHVDEIMARGERTWLDDHGIERDFSC"
     gene            complement(6950..7891)
                     /locus_tag="VFC01_34315"
     CDS             complement(6950..7891)
                     /locus_tag="VFC01_34315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HZN84732.1"
                     /translation="MKLLRRVITVPMATAAMIGILAVWPVLLVIGGVAGLIARSSLPV
                     RTLGVLMAYAVLELRALWTLLRGGQDCDEFVSDVLERAYTVGRRTLNLGVLLDDASPG
                     PEQLPREVPVIVLSRHCGPGDTLLVAWLLNIHYRLRLRIVLKALLRCEPVLDLVGDLG
                     CLCFLHHRSAKARKQIHDLAASLGGGQALLLFPEGGNFTWGRWRAAIERLRSSGRLRE
                     ARRAWRQTHTLPPRTGGTAAALAGAPDADVLVLTHTGFSPDGRARAWWRLPMNRRLLV
                     RTVLVPAAELPSPDQLAPWLERTWSNVDAWVAGHINS"
     gene            complement(7888..8715)
                     /locus_tag="VFC01_34320"
     CDS             complement(7888..8715)
                     /locus_tag="VFC01_34320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZN84733.1"
                     /translation="MSCHAFVLGGGGLLGAAEAGMASALLDAGVRPDVVCGTSVGAIN
                     GAALAADPTPTGAQMLVAFWAELADEGAFDGSMLRRVAEMVRRRTSLHDNGELRRLLR
                     ERLPAQTFEELVVPFECVAASIEQAREHWFNTGDLIEPVLASCALPGVFPPVRIGDEH
                     YFDGGLVNSIPLDRAVQHGADTIWVLHVGRLEEQLAAPRFLWEVGFVAFEISRRHRFH
                     SDLERVGPEVAVHVLPSGLPQRPAATWSNLRFRDTRRISRRAELAYDATREYLEALP"
     gene            8769..9941
                     /locus_tag="VFC01_34325"
     CDS             8769..9941
                     /locus_tag="VFC01_34325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HZN84734.1"
                     /translation="MALARRLCVLAALSSLLAACSSPAQAPAPSSSASSPTMAATGGP
                     APAAEPPMPACGDGPALLASMSTRDKLAQLLMVGVTGAADARAVVDNHHVGGIFIGSW
                     TDLSILSDGTLANIAATGPLPLAVSVDEEGGRVSRLASLIGNQPSPRVLAASHTPEEV
                     YNIALQRGQKMRNLGITIDFAPVVDVTNDADDTVIGDRSFGANPTAVTDYAGAYARGL
                     RDAGLLPVLKHFPGHGHGSGDTHTGSVVTPPIADLQANDLIPYRTLTTEAPVGVMVGH
                     IQVPGLTGSDPASLSPAAYALLRSGDYGGPGFNGPVFTDDLSSMQAISDRFGVAEAAL
                     RGLQAGADIALWVTTDEVPAVLDRLEKAVAGGELNMSQVDGSVLRMAAVKGPNPRC"
     gene            complement(10005..11180)
                     /locus_tag="VFC01_34330"
     CDS             complement(10005..11180)
                     /locus_tag="VFC01_34330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN84735.1"
                     /translation="MPGGELADVFAGVDADVPAALADHLRGIQLVDHHVHGAFEKAVD
                     RAEFEAAINEGSTDPVPSFMTQFDSPLGLAIRRWCAPALGLEPHADADTYWRRRSELA
                     PEELAGVMLRAAGIARWIVDTGYKGDLISTPQRLTELSGVPSSEILRLERLAEDLLES
                     GTQPEAFPGAFRAALQKAADDPATVGTKTIAAYRSSFDIDWSRPADGQVIEHARVLEA
                     RSRPLRLDSPVVIAFGVHEAAEHGLPVQVHVGFGDRDLDLHRTDPMLLLPLLRTMTPV
                     PVLLLHCYPFQRQAGYLAQAFDHVNFDVGLAINYLGVRSTALVAEALDTAPFAKQLFS
                     SDAFGPPELHLLGSVLWRRAMGLVLGDWVRTGDCTESDAIRIVDMIGVHNAARVYGL"
     gene            complement(11167..12462)
                     /locus_tag="VFC01_34335"
     CDS             complement(11167..12462)
                     /locus_tag="VFC01_34335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZN84736.1"
                     /translation="MTTLYDRDEAVIAGIEKLRFFPLEVVSGHGSTLVTPDGRELLDL
                     SATWTASGLGHGHPAVVEAVSRAIRDAPGAGGLSAVHPDSVGLAEDLLALVPGIGERR
                     VYLGHAGSDANDVALRACRHATGRRTVVAFEHSYHGGVGLAMGVSGVHVDAGSPPDAD
                     AVFLPYPNPFRPAQGDVDADVAACLVLAEQHLATGSVACLIVEPILSDGGLVVPPDGF
                     LARLHELCRRHDVPMICDEVKMGLGRPGTLHAFEHDGVVPEIVTFGKVLGGGLPLSAA
                     VGPAALLDEPPAAALLTTAGNPVCTAAGRAVLATIVGENLPERAAKVGAVLQDRLRGL
                     DSDRIGDVRGRGLAIGLELVDPATGDRDARLAAQVVYRAWELGVVVYYVGGNVLEITP
                     PLVLTEAEADRAADILGQAITDAAAGKVDDGEVARYAGW"
     gene            complement(12459..13958)
                     /locus_tag="VFC01_34340"
     CDS             complement(12459..13958)
                     /locus_tag="VFC01_34340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN84737.1"
                     /translation="MTDTTQAQADQIPHRRLPFWVALAVSVALVGPTLAMSGNGQGLI
                     ATVGKSIPLVFLIGLIGVSLVGYSFVRLTRHLNHAGSAYALVGGTIGPRTGFFSGFAM
                     LGAYWGFAIGTLALTAAFTNAFIAELQPGNDNPYQVPWMVVVVVAAAISFVLSGRDIR
                     LLAKILLAIEGIGILAMVVLVIVIFARGGAPSTGIDFSVFSFSGSDVSASAVISGVVA
                     AFLSWAGFEACASLGEETDNPGRNIPRALAGTLALTGVLFVVVMFAQVIGFGTDEAGL
                     AAFQSSGNTLGDLGSTFIGQWFSLIIILTAIVSAFGCHMATTAASARMLYAFGRDGFG
                     PRALAHIHESTGGPRRATWLVVAVALVVNLICGAVGWPDMGTGNDAIDTYFLFAVAGS
                     VCLMVSYLLVDIAAAWFVGAPKFVGVHGGRAKALGLTLPLLGALVIVVVLWFNVKGAE
                     TWSAAPLLGLYWCALGLVIAIAASGIAKRVGESLIRELDLTPPVQASAQ"
     gene            complement(13981..15339)
                     /locus_tag="VFC01_34345"
     CDS             complement(13981..15339)
                     /locus_tag="VFC01_34345"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZN84738.1"
                     /translation="MPVPSDGRRADEDAELARRTAELRATGVELVAGTVTDLAGVTRA
                     KYVPMRRLGAFQQAGMGVSPSWSVFCVDSGIAFTPEIGVVGDLRIRIDPADLRQVDDG
                     VAWAPGDLRDQQGNPAPLCVRSLLAQTEAGLADRGLTARVGAELECTMLAPDGDHAST
                     DPWSPYGVRTSLDRSAFLVDLALAADRAGLAIEQIHTEYGHDQLEVSLAPTTPVAAAD
                     AVILGRIVIGRAAARHGLKISFSPVPFVGEAGNGAHLHLSLADREGPLFSGGDGPHGM
                     RPAGQSAVAGVLEGLPDLLGVYAGSVLSGSRLRPGNWAGAAKCWGLENREAAVRFIAA
                     TPGNPHGANAELKIIDPSANPYLAVAALLGSALRGIDKCLDLPAEVPDNPSESAAAPP
                     PLPTDQRGAIEGLEVSAIAADLLTPAVIEALAAVRRHEITTYGDRSPSETIQALRLAW
                     SC"
     gene            15457..16254
                     /locus_tag="VFC01_34350"
     CDS             15457..16254
                     /locus_tag="VFC01_34350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HZN84739.1"
                     /translation="MCRLLGVVSTSPIAVSDAVGAHVLKDFVALTKIHGDGWGIAHAG
                     RAGQPPRVEVSAGSALDDPAFDAATRNHRWAASLVHLRWATNGIAVQPRNSHPFLADG
                     VAMAHNGSIKPTAALDTLIDPSITATLGGTTDSERYFALIRQHRPAAPDLAEAVRRAV
                     AQLREFYPDTSLNALVLGEDQLIAVHAHARSRLPDEDIKEITAAELPPEHLEDYFALR
                     WARPDEHTIVVGSTGFGDLDWQPLPPESVTSISMRDLSMSLHPLVTG"
     gene            16260..17114
                     /locus_tag="VFC01_34355"
     CDS             16260..17114
                     /locus_tag="VFC01_34355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HZN84740.1"
                     /translation="MIDEPVSARTSAALPALSKAERRVGRALLADYPSAGLASAATLA
                     ERAEVSPPTVLRFAQSLGYEGFTDLQVALRAELSARSSGPIARLPDAPAAGSILERLL
                     QQARAQNDRAAETLAQLPASALEAAVTLLADTRRAVYLHGGRFSHLLAIHLAAHLEQL
                     RPGIRVLDDPSGRDLGVMLEMSRTDVVVLFDYHRYQRSAAELASWVHRAGATVLLITD
                     DLACPVAPDAEVVLAASSTVGTTYQSMAAGFLLTELLIPLVMDAIGEPARTRMALWEE
                     QLRAELLP"
     gene            17172..17795
                     /locus_tag="VFC01_34360"
     CDS             17172..17795
                     /locus_tag="VFC01_34360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011777579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN84741.1"
                     /translation="MAGGTKRLPRAVREQQMLDAAVQMFSVNGYHETSMDAIAAEAQI
                     SKPMLYLYYGSKEELFGACLDRELTRFVDDVRTKIDFSQSPKELLRNAVLAFLTYIDA
                     NRASWMVLYTQATSSQAFAHTVREGRERIIDLVGRLLRSGTRHPEPDTDFDMMAVALV
                     GAGEAVATRVSTGDADVDEAAELMINLFWRGLKGTPSDKDAQAVASN"
     gene            17845..19701
                     /locus_tag="VFC01_34365"
     CDS             17845..19701
                     /locus_tag="VFC01_34365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZN84742.1"
                     /translation="MAETTTPSGRLVWRSLGMLRAVRGLIVVLIPLGVVASALPYVTA
                     AAFGPMMQVVADAGARGNLSGVWDLKGPLVAREDGLLRSLAGPVPFAVLLATWGTSLL
                     LTQLMYLINASVRVKAQRVLVTDIRQRVHDHIQTLSLDFFTGSGSGALIPRVTTESAG
                     VQRLLMDCLLAPLIDAVVLAVAIAYLLALSWQMTIAALALTPVALLTLRFAGRNLQAV
                     IQRVMNADRVMAAELEQTVNGIAEIQTFNAQPIRSKRFQVASENAAKSDASTVVWMQA
                     TANGSQVFIALSIVVALLVGIGFSASFGLTFAGLMVFAGMVPTMFGAAQRVMGAYTTY
                     QSVLPSAASTYELLDTRPSVEDRADAIPLGDVHGNLVFEDVAFGYTPGQNVLDGLSFS
                     ITEGETVALVGGIGSGKSTVFNLVLRFLDPQRGRILLDGHDITAVTMASLREQVSKLA
                     QFPFFTKDTIRENIRLARPDASDIDIEKACEDAHVHSVITDPAKLRDGYDTVVDVQVP
                     SGGQKRLIALARCLLRKPEVLLLDEPTENLDADQRARLKGVIRGYARDRTCMVISHDM
                     DFIAAVADRILVLEHGRIAQVGDHHTLMAEGGLYKRLYEAQNVDPDLVYPSG"
     gene            19713..20678
                     /locus_tag="VFC01_34370"
     CDS             19713..20678
                     /locus_tag="VFC01_34370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HZN84743.1"
                     /translation="MNVITMGAHILDVLVRPVTDIPPGQETALVEQIRMTVAGTAAGT
                     ALTFAKLGATVQTAGAIGTDPAGDLLLSLLGRAGIDTSIVVRKPDVPTSMSVLPIRPN
                     GERPALHLLGANLSYTLDDVDWDAVAAADHIHLGGTEMLGPDFAARILKHAKENGVTT
                     SVDLIAPGGMGTFDLIAPAMAYTDYLLPNEDQVLGFTGATDLDSGSRKLLDAGAGLLA
                     ITCGADGAFVVDDEGKQQVPAFTVNVVDTTGCGDAFSAGFVYATGIGRPPRDAAVLGN
                     AVAALVAQGLGSDHGEFDLAAADAFAASQAVGNPRASGSDPAERG"
     gene            complement(20689..21126)
                     /locus_tag="VFC01_34375"
     CDS             complement(20689..21126)
                     /locus_tag="VFC01_34375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6319 family protein"
                     /protein_id="HZN84744.1"
                     /translation="MTVDTLPREAPSTDSDAPDVLVPSVESISPAPDTSADAASVEEK
                     PKKVQAKKAAGKKTKTLELTLTVTGTADGEWRAELKQGTTYLARNLTVAAAAVSRAAK
                     ELHEDLSTPIDEVIEEARSQQAAKVAALEAELEAARKALADLE"
     gene            complement(21275..21403)
                     /locus_tag="VFC01_34380"
                     /pseudo
     CDS             complement(21275..21403)
                     /locus_tag="VFC01_34380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
     gene            complement(21585..21989)
                     /locus_tag="VFC01_34385"
     CDS             complement(21585..21989)
                     /locus_tag="VFC01_34385"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HZN84745.1"
                     /translation="MVSQGANSEECNVPRQRFDPYAVLGLSPTATPDEITHAYRTQVR
                     AFHPDTRTTPESTPAPADEQLRRVFAAYALLRDPQRRARYDRQAARYNRATQRRPTVP
                     RAPAETTNTVDLVGTVRILGLTFGFRIGRQSD"
     gene            complement(22151..22993)
                     /locus_tag="VFC01_34390"
     CDS             complement(22151..22993)
                     /locus_tag="VFC01_34390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HZN84746.1"
                     /translation="MTQPSGLMNLVRAAAGALPFVPRGDTLPDRTLTVDELAIDPANV
                     AAYANVTGLRFGDAVPLTYPFALTFPTVMSLVTGFDFPFAAMGSVHIENHITQYRPIL
                     VTDTVSVRTHAENLREHRRGLLVDIVTDVNVGNETAWHQVTTFLHQQKTSLSDEPKPP
                     PQKQPKPGPPNAVLRITPGQIRHYASVGGDHNPIHTNPVAAKLFGFPTVIAHGMFTAA
                     AVLANVEGQLPDAVKYSVKFAKPVVLPARAGLYVDRISDGWQLTLRNLSKGDPHLTGT
                     VTSL"
     gene            complement(22995..24344)
                     /locus_tag="VFC01_34395"
     CDS             complement(22995..24344)
                     /locus_tag="VFC01_34395"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HZN84747.1"
                     /translation="MASDLYSQIVHSTPGSFLAKQLGIPQPETLRRYKPGDQPLAGTL
                     LIGGEGRVVEPLRTALAEDYDVVSNNLGGRWADSFGGLVFDATGITEPAGLKGLYKFF
                     TPLLRNLGPSGRIVVIGTTPDEISGHERIAQRALEGFTRSLAKELRRGATVNLVHLSP
                     DAKPAATGLESTVRFILSGKSAYVDGQVFRVGAADSAPPADWDKPLDGKVAIVTGAAR
                     GIGATIAEVFSRDGAKVVCIDIEGAAEPLGVTASKVGGTALTLDVTAEDAVDKITEHL
                     REVHGGRADILVNNAGITRDKLLANMDESRWDSVVAVNLLAPLRLTEGLIDNGSLGEG
                     GRVVGLSSMAGIAGNRGQTNYAATKAGMIGLTDALSEAYADKGITVNAVAPGFIETKM
                     TEAIPLATREVGRRLNSLYQGGQPVDVAETIAYFASPASNAVTGNTIRVCGQAWLGA"
     gene            24391..25689
                     /locus_tag="VFC01_34400"
     CDS             24391..25689
                     /locus_tag="VFC01_34400"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZN84748.1"
                     /translation="MANQSSRRVAVLGGNRIPFARSDGAYANASNQDMFTAALGGLID
                     RFNLEGEKLGAVIGGAVLKHSRDFNLMRECVLGSSLSSYTPAFDIQQACGTGLQATIA
                     AADGVAAGRYDVAAAGGVDTASDAPIAFGDDLRRVLLGLRRSKSNVERLKLVGKLPAS
                     LGVQIPVNSEPRTGLSMGEHQAITAKQMGIKRVDQDELAAASHRNMAAAYDRGFFDDL
                     VTPFLGLYRDNNLRADSSPEKLASLRPVFGVKAGDATMTAGNSTPLTDGASVSLLATP
                     EWASAHGIEPLAYYVDAETAAVDYVNGKDGLLMAPTYAVPRLLARNGLSLGDFDFYEI
                     HEAFASVVLAHLQAWESEQYCKERLGLDAALGSIDRSKLNVNGSSLAAGHPFAATGGR
                     IVAQLAKQLAEKKKETGQPVRGLISICAAGGQGVAAILEA"
     gene            25828..>26573
                     /locus_tag="VFC01_34405"
     CDS             25828..>26573
                     /locus_tag="VFC01_34405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03564 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZN84749.1"
                     /translation="MQISLSLVGLLSDSTGASPVDGTIKNLARLRDEGFRRVWMAQLP
                     YDPDLLTILAVALHEVDTIEVGSGVVPIQVQHPTQLAQRALTLNAIAGGRFSLGIGMS
                     HKMVTEQMWGISYEKPLRRMREYLDGLLPLLSGQAADAVGETVTTRGSLQIPGAPTPD
                     VYIAALGPQMLRLAGRRTAGTLTWMTGPKTLAEHVGPALREAAAEAGREESAVRVAAS
                     LPVSVTDDIDAARARAAEEFAIYGQLPSYRA"
BASE COUNT         4535 a         8965 c         8839 g         4234 t
ORIGIN      
        1 ccttcttcgg cgaaaatgcg ctgctgctcg agaccccggc gggtggccgc gtgggtgtga
       61 gtcgagatcg gcacgccggt gagacgatgc gccttggcga cggcccgcag cacgcgctcc
      121 acgccagggg tgacgcccgg ctcgtcggtc gcacacttga ggatcgcggc cttgaccccc
      181 gtgtcggcga tgccgtgctc gatgtcgcgg acgaacatgt cgaccatcgg ctcgccgtcg
      241 ccgagtgcgc ccccaggtcc ggtgaagtgg aagtacatcg gcacgtcgtt gtaggtgtac
      301 acgcccgttg ccaccacgat gttgatgtcg gtggccgccg cgacgcgctc gatccgcggg
      361 atgtagcggc ccatcccgat cactgtgagg tcgacgatgc tgtcgacacc gcgcgacttc
      421 agttcgttca gccgccttat ggcgtcgtct tctcgcttgg cctcgtcacc ccagacctcg
      481 ggatagttcg cggtgatgtc gggcgacaag atgaacacat gctcatgcat gagcgtcacg
      541 cctagacctg atgtatcgat tgctccccgt gcggtgttca gaatcggcac cccgccgatg
      601 ctaggacgat cgcgatttct ccgggccgct atcgtggcga atggaggtgc ctcatgccga
      661 cagccgccca gttgatggtc aagtgcctgg aaaacgaggg tgtttcagtc gtattcgggg
      721 tgccgggcga ggagaacatt cgcttcatcc aggcgctcgc cgcatcgccg attcgctatg
      781 tactcacccg gcacgagcag ggcgctgcgt tcatggccga gatgtacggc cgggtcaccg
      841 ggcgggccgc cgttgcttcg gcgacgctgg ggccgggtgc catcaacatg cagctgggcg
      901 tcgccgacgc caccaccaac agcaccccgc tggtcgcgat ctctgctcaa gtcggccagg
      961 accgcgagta caaggagtca catcagtacg tcgacctggt atcgatgttt gcccccatca
     1021 cccgctgggc tgacggcgtt ccgacgtccc gcgcgatacc cgagatggtc cgcaaggcat
     1081 tcaaggtagc cgagactgaa cgccccgccg cggtctacct cgccgtgccc gagcacatcg
     1141 acgccgacga aacggattac gacctaaccc ccttgccgcg caacgttgtc cgcgccgaag
     1201 cgccgtcgcc cggacaggta cagcgtgcgg tcgaggttct gcgcgatgcg aagcggcccg
     1261 tggtgttggc aggccacggt gccgcacgca gtgacgcgac tgcagcgctg gtccggttct
     1321 ccgacgaact cggtgtgcag gtggcgaaca ccttccacgg caagggcgtg atgcccgatg
     1381 accaccccaa cagcatcggc accctggggt tcatgcgccg cgactatgtc aacttcggat
     1441 tcgagacggc ggacgtcgtc attgcggtcg gatacgaact gcaagagttc gatccggtca
     1501 ggatcaaccc gaacggtgac aagaagatca tccatattca tcgcttcccg gccgaggtcg
     1561 acatgcacta cccggtttcg gtcggcatca tcggcgacat cagcgagtct ctgaacgcgc
     1621 tgaccgacgc gttagccggg cacagatacg acaccgccga cgccgcacca gggggcggtc
     1681 tgctcgccgc tgaattcgca cggggacagc aggattcgcg gttcccgctc gcaccgcagc
     1741 gtatcgttgc ggacactcgc gccgctctcg gccgtagcga cgtcgtgctg gtcgacacgg
     1801 gcgcaacaaa aatgtggatg gcgcggctgt acccgaccta tgaatgcaac acctgcctga
     1861 tttcgaatgg cttgtccacc atggcgtttg cattgccggg cgctctcggg gtcaagttgg
     1921 ccaagcccga cgtgaaggtg ctggcgctgg tcggcgatgg cgcctttctg atgaactcgc
     1981 aggagatcga gaccgcggtg cgcgagcgga taccgctggt ggtactcatc tgggaggacg
     2041 gcggctacgg gttgatcgag tggaagatgg acctggagtt gggcgagcac cactacgtct
     2101 cgttcaccaa ccccgatgtg gtgacgtacg ccgaaagctt cggtgcgaag gggtaccgca
     2161 tcactgaggc tgatcagctg ttgccgacgt tgaagaaggc acttgctgac gacggagttt
     2221 cgctgatcgc ctgtccggtc gactactccg agaacctgcg tctgacagac actctaggtc
     2281 agctggacga gacgttgtag ccgcagaagc ggccagcatc accagcgaga tcagtgccag
     2341 cagctgtaca cccgccgagt gtccggcata gccgtcgacc gaccgccacg ggttctggct
     2401 cagcaccgct cccgcgacga tcagcccgcc cgcggcgacg ccgaccgtga ctgcttcaca
     2461 aagcttttcg cgccgccgaa gcaggtagcg caggcccact gccgcgccgc cgacgatgac
     2521 gcctgcgatc ccggagatca tcgccgcgac cgccaccaca gcgacgctcg tcgtcatccg
     2581 tcccggctgc cacggccgcg caggttcgtc ggtgggacgg gggcgtcgaa ccggcaggaa
     2641 ggcaagcaac gcgagcaatg gaagcagtgc cagcccgccg atcagcccgg ctcgataggg
     2701 cgtgttcgac gcaaagctca gcgcgacggg tcccgacgtg cccgggggaa gcacccagcc
     2761 ctgctgccac ccgttgacgg tgaccggggt cagcgcagca ccgtcggccg cgcgggccgt
     2821 ccagcccgga ttgatgcttt cgggcaccac caggacccga gacgacggag acgccgcgac
     2881 attgacctcg cggtggtcgg caccccagtt gcctgtggcg gcgggaaccg ttgccgctgt
     2941 gggcaattgg tttgccaggg gtccggccag ctgcacaccg tcgaccacga acgcggagcc
     3001 ggggctgatc agcagctcct gctgaccggc gggcagcgtg atcggctcgg accgacacgg
     3061 cgtggcggcc acaggctcgc catcgagcag cgcgccgacg gtggtgacga tcgaggtctg
     3121 gacgaactgt ccggccacgc cgatgatcgg acccttcccg cacggcagat caataacacg
     3181 cgctcgattg cgcgcggcgt cggcgccggc gatcggcatg cccctggcat ccagcgcgga
     3241 gacctcggcc agaccgggtg gcttgagctg atcgaagccg agcgcggtgc ggtcgatcac
     3301 gtcgttccaa ctcaacagcg acagctttac cgtgtcggtg acatgtggtc gcaggctcac
     3361 cgactgagta ccgccgtcgc tggcgagcgc gcgcacctcg gggccgccac cgaggtcgat
     3421 ggccaccagt gtcggatgtg cgggcagcac tgaggaactc ggcgtgatcc gcaggcccgc
     3481 cacctcgcgg ggggcgggga atttcagggt gagcgtgggc ggcgtcttgt gttgcacgac
     3541 gccttgcggc gcggtccagg cggtcgcggg atcgccgtcg gcggcggcgt atgccgaacc
     3601 ctgtacgtcg atgggatcgg cgtcgccgag cgcccgcgcc gtgccgggtt cgctgatcaa
     3661 gtcggcgaga ttcgggcctt gacgcgcgcg cacccacacc gtcggcgtca cctcggtcgg
     3721 cgacggcacg gtcagcgtcc ggctcaggtt caccggctct tctggcgaca gcgccatcga
     3781 cgctgcacac cgtgtgccgt tgggagcctg ggcgcaaccc ggacggccga gcagctcgga
     3841 ccccagatcc cattgcgcca cagcagaatt gggtggtggg ccaggcacct gcaccgtgtg
     3901 gcgcaggctg acgggatggg cgaagccgtt cgcgtcgtac tgcgtgacgg tgaaatcggt
     3961 gatgccgaac tgcaccccgg acgacccgtc gtcagtggcc accgcggtga tccgcaccca
     4021 tggcgtctcg ccgtagggca gcgcgatcgt gagcggcttg ccggcctgtt cgaagcgcgt
     4081 ggtgctggtg ccgttgacgg tcgacacctc gatgcggcgg atctgcgcgc cgacggcggt
     4141 ggcgctgggc gtgatcgtga tggtggcgtt ggtgaccgga tggtcgaagt cgacctgcag
     4201 ccattgcccg accgccgcct gtagcgcgtt ggacacccag ctcgtcgacg tatcggcgtc
     4261 gatggccgcc gcaggcccgg ttgcctgtgc gacgttgggc agcgccgtgg aatccgatgc
     4321 cgaactcgac acggagactc gcccgccgtt ccactgcccg tagaccaggt cggcgccggc
     4381 tgcgggatag tcgggcacgc ggttgaacgt gtgccgggga tcgtcggggg tccggatcgc
     4441 cgacgagtgg tcatcgacgc ggccgtagtc cgtttcgcgc gccaacgggg tgtcggtcac
     4501 ggtgacccct tttcctgggg gcaacgttag gccggcgcgc tgggcgtcct gggtgagcag
     4561 catcggcccc agcggcggcc ggccgagcag ccggcgtcgc tcgtcgagcc gcagcagtgc
     4621 ctcgggtccg ccgtcgaccc gcgccgtcgc ggctgtgtcg accaggtacg gatgcatccc
     4681 cggtgagccg tcgactcggt agatctccac tgccgggaac cgcggccgca gtccgctgtc
     4741 ggtgatgaac ccggccagcg ttcccgggcc gacaggctcg ccgaactgcg ccactctgac
     4801 gagtccaccc gatccttcga tcgtgcggtg caccagcagt ggacgcgccg accgcgacgc
     4861 ctcgggatcg aggtcgttgc ggaccaccac atacaaaata ccttgccggg caagcgtatc
     4921 cgccagaccc gcggacggcc ggcccgcggc gaacagccgc tgcacagagt cgagagcgcg
     4981 gatcgtctcc ggtggggtca gcggaatcga atcgcgtact ccccacgggc tgctgcccag
     5041 cacctgcagc ggttcgtcgt ggctgttgcc ccacacctgc gtcgcgaacg gcgcgccggg
     5101 cgccaccagc acgcggccgc cgtctttgtt gtgttcgtcg agccagtcgg cggtgtcatg
     5161 ccaatactgc gggatcgcgt cgaaggcgcc aggtggggta agcctgccgg tccaggccag
     5221 cgatgtggcg gcggccaggg caacaagcac caccactccg acggccactc gcttgtcgcg
     5281 ttcgggatga gcgaacgcat gcatccactc gcgccggggc acgctgccgg gcaacgggat
     5341 tcggcccagc agatgcgtca gcccgagcat cagcggcagc cgcagtagcg gctccagctt
     5401 gtgcatgttg cgcagcggag tgccgtctgc atcgaggaac gcctgaactg cgtgcgcgac
     5461 aggtgatccc agcccacccg aatagccgac cgcgagcagc acgacaccga caagcaggat
     5521 cacaatgagc cgaccgcgcg cgggcatcgt gcgcatcgcc agtccggcca tgcctgcggc
     5581 cgcgaccaac gtcgtcgcca acaccgcgac cgagcccgtc accaacgacg cgccggcggt
     5641 ggcggtcggc gcgacaaacg gtgtccaggc gtcggtgccg cgcaatatct cggtcagcga
     5701 catccattgc gtggtgacgc cggaggattc gatgaagtcg aggaacggcg gactgatccg
     5761 gcccagcatg acgagcgcga ccacccacca cagcaccgcc agcacgatgc acgccgccca
     5821 ccacgcggca aagcgcaacc acaggcggtt cggctgatgg cagaccagcc agatcaccgc
     5881 cgccagacag ccggtcaacg tcgccaccgc gttgaccgcg cccatcaacg cgatcgccaa
     5941 cgcggaccgg gcggccagca cccgcaccga accctgaccc ctcagcgcga ggatcaccgg
     6001 cagcagcacc caaggggcca gcatcatcgg cagcgtctcc gacgagatcg cgcccagcgt
     6061 ggtcagcacc cgcggcgaca gcgcgaacgc ggccgcgccg atgacacgcg acgtcgaatt
     6121 accgatgccc agcgcctcgg cgactcgcaa caagccccag aaaccgaccg tcagtagcag
     6181 cgcccaccac agccgttgcg tcacccagcc gggcaggcca agcacgtcgc cgagcaggaa
     6241 gaaggtgccg tgtgggaaca ggtagccgta ggcctggttc tgcgcctggc cgaacggcag
     6301 ctcgctgttc cacaggttga acgcgcgggc gagaaaccgc agcgggttcg ctgtgaggtc
     6361 gagtttggtg tcgggtgaga tctttccggg cgactgggcg aaagtcagga gcagcgccgc
     6421 cgcggcggcc atccaaagcc atcgccgcga gagcggcgcg gtcacgctag gttctgtcgc
     6481 cgtactcgac cctgttgagc accgacgagg ccggatcgcc tgcctgcagc ggaggcttgc
     6541 tgtcttgctg caccatcagc gtcacaccga aaatggcggc cgctcccagc agcaggccga
     6601 caacgatgct ggccgcggcg ggaacgatga accgatccat gcggccaacg tagcagacaa
     6661 tgttttgcgt gcccgccacc gaacgcgtct tctggcggac cgataccagc acggtgtggg
     6721 tcggttatga cgacgacggg gcgctgggtg ttcaacggcc tcgaccgggc gtatctcgac
     6781 ggctacgagt acgccgtcac ggtggcgccg gatcaattcg ccaacctgcg acgggtactc
     6841 ggcgccgact cgggcgccga cgtcgtcgac ctcgtctgcg cccacgtcga cgagatcatg
     6901 gcgcgcggcg agcggacctg gctggacgac cacggcatag aacgagactt cagctgttga
     6961 tatggccggc gacccacgcg tccacgttgc tccacgtccg ttccagccac ggagccagtt
     7021 ggtcggggga gggaagctca gcagcgggaa ccagcacggt gcggaccagc agccgccggt
     7081 tcatgggcag gcgccaccac gcccgtgccc gcccgtccgg tgagaaaccc gtgtgggtga
     7141 gcacaaggac gtctgcgtcg ggcgctccgg ccagcgccgc agcggtgcca cccgtgcgcg
     7201 gcggcagcgt gtgcgtctga cgccaggccc gacgcgcctc gcgcagccgg ccggacgacc
     7261 ggagccgctc gatggcggct cgccaccggc cccaggtgaa gttccctccc tcggggaaca
     7321 acagcagcgc ctgcccgccg cccaacgatg ccgccagatc gtgaatttgc ttgcgcgcct
     7381 tcgcacttcg gtgatgcagg aagcacaggc agccgaggtc gccgacgaga tcgagaaccg
     7441 gctcgcaccg cagcagggct ttgagcacga tccgcaaccg cagccggtaa tgaatgttga
     7501 gcagccaggc caccagaagc gtgtcgcccg gaccgcaatg ccgcgacagc acaatcaccg
     7561 gaacctcgcg tgggagctgc tccgggcccg gcgaggcatc atcgagcagc acgcccagat
     7621 tcagggttcg gcgcccgacg gtataggccc tttccagcac gtcgctgacg aactcatcgc
     7681 agtcctgccc gccgcgcagc agtgtccaca acgccctaag ttccagcacc gcatacgcca
     7741 tgagcacgcc gagtgtccgc accggcagac tcgaccgagc tatcagcccc gccacaccac
     7801 cgatgaccag cagcaccggc cacaccgcga ggatgccgat catcgcggcc gtcgccatcg
     7861 gcaccgttat cacccgacgc agcagcttca tggcagggcc tcgaggtatt cgcgcgtggc
     7921 gtcataggcc agctcggcgc gccgggaaat gcggcgggtg tcgcggaagc gcagattcga
     7981 ccacgtcgcg gcaggccgct gcggtaagcc gctcggaagg acatgcacag ccacctcggg
     8041 gccgacccgc tcgaggtcgc tgtggaagcg atgcctgcga gagatctcga acgccacaaa
     8101 cccgacttcc cacaggaacc ggggcgcggc gagctgctcc tcaaggcgac cgacgtgcaa
     8161 cacccagatc gtgtctgcgc cgtgttgcac cgcccgatcc agcggaatgc tattgaccag
     8221 accgccgtcg aaatagtgct cgtcaccgat tcggacaggc gggaaaacgc ctggcagcgc
     8281 acaagatgcc agcaccggct cgatcagatc gccggtgttg aaccagtgct cgcgagcctg
     8341 ctcgatgctg gcggcgacgc attcgaacgg cacgacgagt tcctcgaagg tctgcgcggg
     8401 caggcgctca cgcaatagcc ggcgcagctc gccgttgtcg tgcaacgacg ttctgcggcg
     8461 aaccatctcg gcgacccggc gaagcatcga cccgtcgaat gcgccctcgt cggccaactc
     8521 ggcccagaac gcgaccaaca tttgcgcgcc ggtcggcgtg ggatccgccg ccagtgccgc
     8581 gccgttgatc gcgccgaccg atgtaccgca caccacgtct ggccgcaccc cggcgtccag
     8641 cagtgcgctg gccataccgg cctcggcggc tcccaagaga ccgcccccac ccagcacgaa
     8701 ggcatggcac gacatgtggc aggaataccc acgaagcggc cgctactagc gcatgacagc
     8761 atggcggaat ggcattggct cgtcgactgt gtgtcctggc ggcgctgtct tcgcttctgg
     8821 cggcctgttc gtcgcctgct caggcgccgg ccccctcgtc gtcagcctca tctccgacga
     8881 tggccgcgac cggagggcct gcacctgctg cagagccgcc catgccggcg tgcggtgacg
     8941 ggcccgcgct gctggccagc atgtcgaccc gagacaagct ggcgcagctg ctgatggtcg
     9001 gggtcaccgg cgcggcagac gcgcgggccg tggtcgacaa ccatcacgtc ggtggcatct
     9061 tcatcggcag ctggaccgac ctctcgatac tgtccgacgg cacgctggcc aacattgccg
     9121 cgacgggccc gctgccgttg gccgtcagcg tcgacgagga gggcggccgg gtgtcacggc
     9181 tggcctcgct gattggcaac cagccgtcgc cgcgagtgct ggcggccagc catacgcccg
     9241 aagaggtgta caacatcgcg cttcagcgcg gccagaagat gcggaacctc ggcatcacaa
     9301 tcgacttcgc tccggtcgtc gacgtgacca acgacgccga cgacaccgtg atcggcgacc
     9361 gctcgttcgg cgccaacccg acggccgtca ccgactacgc gggcgcgtac gcccgcgggc
     9421 tgcgcgatgc tggcctgctg ccggtgctca agcacttccc cggccacggc cacggctccg
     9481 gcgatacgca caccggcagc gtggtgaccc cgccgatcgc ggatctgcag gccaacgacc
     9541 tgatcccgta tcgcacgctg accacggagg ccccggtcgg ggtcatggtc ggccacatcc
     9601 aggtgcccgg cctgaccggc agcgacccgg cgagcctgag cccggcggcg tacgcgttgc
     9661 tgcgttcggg tgattacggc ggcccggggt tcaacggccc ggtgttcacc gacgacctgt
     9721 ccagcatgca ggcgatttcc gaccggttcg gcgtagcgga ggccgcgctg cgcgggctgc
     9781 aggccggcgc ggacatcgcg ctgtgggtga cgacggacga ggtcccggcg gtgttggatc
     9841 ggctggagaa ggcggtcgcg ggcggggagc tgaacatgtc gcaggtggac ggttctgtgc
     9901 tgcggatggc cgcggtcaag ggcccgaatc cgcgctgctg aagccgcact caccgaacaa
     9961 ctttgacgac gtccagtgcc gaacagcggc gcggtcgcgt gctttcaaag cccgtatacc
    10021 cgcgcggcgt tgtggacgcc gatcatgtcg acgatgcgga tcgcgtcgct ctcggtgcag
    10081 tcgccggtgc gtacccagtc gccgagcacc agccccattg ccctgcgcca caacaccgag
    10141 ccgagcaggt gcagttcggg cgggccgaac gcgtcagagg agaacagctg cttggcgaac
    10201 ggcgcagtgt ccagcgcttc ggcgaccaac gcggtcgaac gcacacccaa gtagttgatg
    10261 gcaagtccca catcgaaatt cacgtgatca aaagcctgcg ccagatagcc ggcctgacgc
    10321 tggaacggat agcagtgcag cagcagcacc ggcaccggcg tcatggtccg cagcagcggc
    10381 agcaacagca tcgggtcggt gcgatgcaga tccaggtcgc gatcgccgaa tccgacatgc
    10441 acctgcaccg gcaatccatg ttctgcggcc tcgtgcactc cgaacgcgat caccaccgga
    10501 ctgtcgaggc gaagcggcct cgaccgcgcc tcgagtacgc gggcgtgctc gatcacctgc
    10561 ccgtcggccg gacgcgacca gtcgatgtcg aacgagctgc ggtaggccgc aatcgttttg
    10621 gtgcccactg tcgccgggtc gtccgccgcc ttctgtaggg ccgcccggaa cgcgccgggg
    10681 aaagcctcgg gttgggtccc ggactccagc aagtcctcgg cgagccgttc caaccgaagg
    10741 atctccgacg acgggacacc gctcagctcg gtcaggcgct gcggcgtgga gatgagatcg
    10801 cccttgtagc cggtgtcgac gatccacctg gcgatgccgg cggcgcgcag catcacgccg
    10861 gcaagttctt ccggagccag ttcgctgcgg cgtcgccaat aggtgtcggc gtcggcgtgc
    10921 ggctccaagc ccagcgccgg cgcgcaccag cgccggatcg ccaagcccag cggcgaatcg
    10981 aactgcgtca tgaacgacgg caccggatcg gtggagccct cgttgatcgc cgcctcgaat
    11041 tcggcacggt caacggcctt ttcgaaggcg ccatgcacat gatggtcgac cagttggatg
    11101 ccgcgcaggt gatcggccag cgccgcgggc acgtcagcgt ccaccccggc gaacacgtcg
    11161 gccagctcac cacccggcat accgggcgac ctccccgtca tcgaccttcc cggcggccgc
    11221 atccgtgatg gcctggccga gaatgtcggc ggcgcggtcc gcctcggctt cggtcaacac
    11281 cagaggcggc gtgatttcca gcacgttgcc gccgacgtag tacaccacga cgcccaactc
    11341 ccaggcgcgg tacaccacct gcgccgcgag ccgggcgtca cgatctccgg tcgcggggtc
    11401 gacgagctcc agaccgatcg ccagtccccg tccacgaacg tcgccgatgc ggtccgagtc
    11461 cagcccgcgc aaccgatcct gcagcaccgc accgactttc gcggctcgct ccggcaagtt
    11521 ctcaccaacg atggtggcca ataccgcgcg gcctgccgcc gtgcacaccg gattgcccgc
    11581 cgtggtcagc aaggccgcgg cgggtggctc gtcgagcaac gccgcgggtc cgacggccgc
    11641 cgacaatggc aggcccccgc cgagcacctt gccgaacgtg acgatctcgg gcaccacacc
    11701 gtcgtgctcg aacgcgtgca gcgtgccggg gcggcccagc cccatcttga cctcgtcgca
    11761 gatcatcggc acatcgtggc ggcggcacag ttcgtgcagt cgggccagga agccgtccgg
    11821 cggcaccact aggccgccgt cggacaggat cggctccacg atcaggcacg cgacggagcc
    11881 ggtcgccaga tgctgctcgg cgagcaccag gcaggccgcg acgtcggcgt cgacgtctcc
    11941 ctgcgccggt cgaaatgggt tggggtaggg caggaatact gcgtcggcgt ccggcggtga
    12001 ccccgcgtcg acgtgcacac ccgagacgcc catcgcgagg ccaacgccgc cgtggtagct
    12061 gtgttcgaac gccacgaccg tgcgccgccc cgtggcgtgt cggcaggccc gcaatgcgac
    12121 gtcgttcgca tccgagccgg cgtgaccgag atagacccgc cgctcaccaa tgccggggac
    12181 cagtgcgagc agatcttcgg ccaggcccac cgaatcgggg tgcaccgccg acagtccgcc
    12241 ggcgcccggc gcgtcgcgga tcgcccggct gaccgcttcc acgacggcgg ggtgaccgtg
    12301 ccccagcccg gacgccgtcc aggtcgccga caggtccaac agctcgcgcc cgtcgggggt
    12361 taccagggtc gagccgtgcc ccgagacgac ctccagcggg aagaaccgca gcttctcgat
    12421 gccggcgatg acggcctcgt cgcggtcgta cagcgtggtc attgcgcact cgcctgtact
    12481 ggtggcgtca ggtcgagttc gcggatcagc gactcaccaa cccgcttggc gatgccagaa
    12541 gctgcgatgg cgatcaccaa cccgagcgcg caccagtaga ggcccaaaag cggtgctgcc
    12601 gaccaagtct cggcaccttt gacgttgaac cacagcacga ccacgatcac cagggcgccc
    12661 agcagcggca gcgtcagccc cagcgccttg gctcggccgc cgtgcacgcc aacgaatttg
    12721 ggtgcgccga cgaaccaggc tgcggcgata tccacgagta ggtaactgac catcaggcac
    12781 accgaaccgg cgacggcgaa caggaagtag gtgtcgatgg cgtcgttacc tgtgcccatg
    12841 tcgggccatc cgaccgcacc gcagatcaga ttgactacca acgcgacagc gacgacgagc
    12901 caggtggccc ggcggggacc gccggtcgac tcgtggatgt gagccaaggc ccgaggcccg
    12961 aaaccgtcgc ggccgaaggc gtagagcatc cgtgcagagg cggccgtggt ggccatgtga
    13021 caaccgaacg ccgagactat cgcggtgaga atgatgatca agctgaacca ttgcccaatg
    13081 aacgtgctgc ctaagtcgcc caatgtgttt cctgaactct ggaaggcggc cagaccggct
    13141 tcgtcggtgc cgaagccgat gacctgagcg aacatcacga cgacgaacag cacgccggtc
    13201 agggccaggg tccctgcgag cgcgcgcgga atgtttcggc cgggattgtc ggtctcctca
    13261 ccgagcgatg cgcacgcctc gaacccggcc catgacagga aggccgccac tacgccgctg
    13321 atcaccgccg aggctgaaac gtcgctgccg gagaacgaga acacgctgaa gtcgatgccc
    13381 gtcgacggcg cgccgccgcg cgcgaagatc acgatgacga gcaccaccat ggcgaggatg
    13441 ccgataccct cgatggcgag caggatcttg gccagcaggc ggatgtcgcg gccggacaac
    13501 acgaacgaga tcgctgcggc caccacgacg accaccatcc acggcacctg gtagggattg
    13561 tcgttgcccg gctgcagctc ggcgatgaac gcgttggtga acgcggcggt caacgcgagg
    13621 gtgccgatgg cgaagcccca atacgcaccc agcatcgcga agccggagaa gaacccggtc
    13681 ctcggcccga tcgtgccgcc cacaagcgca tatgccgacc cggcgtggtt caggtggcgc
    13741 gtcagccgga cgaagctgta accgaccagc gacaccccga tcaggccgat caggaacacc
    13801 agtggaatgg atttgccaac cgtggcgatg agcccctggc cgttgcctga catggcaagc
    13861 gtcggcccga ccaaggcgac ggacaccgcc aacgccaccc agaacggcaa gcggcgatgc
    13921 ggaatctgat cggcctgtgc ctgcgtggtg tcagtcatcg gtatctcctt aggggatggg
    13981 tcaacagctc caggccagcc ggagcgcctg gatggtctcg gagggggatc ggtctccgta
    14041 tgtggtgatt tcgtgccggc gcacggctgc cagagcctcg atcacggcgg gtgtgagcag
    14101 gtctgcggcg atcgcggaca cctcgagccc ctcgatcgca ccccgctggt cggtcggcag
    14161 cggcggtggc gcggcggccg actcggacgg gttgtccggg acctcggcgg gcaggtcgag
    14221 acacttgtcg atgccgcgta gtgcgctgcc aagaagcgcc gcgaccgcca gataggggtt
    14281 ggcgctgggg tcgatgatct tcagttctgc gttggcgccg tgcgggtttc cgggagtcgc
    14341 cgcgatgaac cggaccgctg cctcgcggtt ctccagcccc cagcatttgg ccgcacctgc
    14401 ccagttgccg ggccgcaacc gcgaccccga caacaccgaa ccggcataaa cgccaagcaa
    14461 atccggcaga ccctcgagca caccggcgac ggcggactgt ccggccggcc gcattccgtg
    14521 tgggccgtcg ccgccggaga acagcgggcc ctcacgatcg gctagcgaca ggtgcaggtg
    14581 cgctccgttg cccgcttccc cgacgaacgg caccggcgaa aatgagatct tcaagccgtg
    14641 gcgggcggcc gcgcgaccga tcacgatccg gccgaggatg acggcgtccg cggccgcgac
    14701 gggtgtggtc ggagcgagcg agacctcgag ctgatcgtgg ccatattcgg tgtggatctg
    14761 ctcgatggcc aggcccgcgc ggtcggcggc gagcgccagg tcgacaagga aggcggagcg
    14821 gtccagtgac gtgcgcacgc cgtagggcga ccacgggtcg gtcgacgcat gatcgccgtc
    14881 gggcgccagc atcgtgcact cgagttcggc gccgacgcgt gcggtgagcc cgcgatcggc
    14941 gagccccgct tcggtttgtg cgagcagtga acgcacacac agcggcgccg ggttgccctg
    15001 ctggtcccgc aggtcgccgg gcgcccatgc caccccgtcg tccacctgac gcagatccgc
    15061 ggggtcgatc cggatccgca gatcaccgac gacaccgatc tccggggtga aggcgatccc
    15121 gctatcgacg cagaagacgc tccacgacgg cgacacgccc atccccgcct gctggaatgc
    15181 gccgagccgg cgcatcggca cgtatttggc ccgggtgacg cccgccaggt ccgtcaccgt
    15241 gccggcgacc agttcgactc ccgttgcgcg caattcggcc gtgcggcggg cgagctcagc
    15301 gtcctcatcg gcacgtctgc cgtcgctcgg cacgggcatt cgacctccat cgagacaacc
    15361 tgagtctgcg gccacgctat gtcataaatg tttcatgatc tttaatattg gcgctagagg
    15421 attgattcat aaaatttggg atcggaggat gcccgcatgt gccggctgct gggtgtggtc
    15481 tcgacatcgc cgatcgccgt ctcggatgcc gtcggcgccc acgtcctcaa ggacttcgtc
    15541 gcgctgacca agatccacgg cgacggctgg ggcatcgccc acgccggccg ggccggccag
    15601 cctccgcggg ttgaggtttc cgccggcagc gcgctcgacg atcccgcgtt cgacgcggcg
    15661 acccgcaatc accgctgggc ggcgagcctg gtccacctgc gttgggcgac aaacggaatc
    15721 gcggtacagc cgcggaactc ccacccgttc ctcgcggacg gtgtcgcgat ggcgcacaac
    15781 ggctccatca agccgacggc cgcgctcgac acgcttatcg atccgtcgat caccgcgaca
    15841 ctgggtggca ccaccgacag cgagcgctac ttcgcgttga tccgccaaca ccgccccgcc
    15901 gctccggatc tggccgaggc ggtgcggcgc gcggtggcgc agttgcggga gttctacccc
    15961 gacaccagcc tgaacgcact ggttctcggc gaggaccaat tgatcgcagt gcacgcgcac
    16021 gcgcgaagcc ggctgcccga cgaggacatc aaggagatca ccgcagctga gctcccgccc
    16081 gagcatctgg aggattattt cgcgttgcgc tgggcccgtc ccgacgagca cacgatcgtc
    16141 gtcggatcca ccggtttcgg cgatctggac tggcaaccgc ttccaccgga aagcgtcacg
    16201 tcaatctcga tgcgcgacct gtcgatgtcc ttgcatccgc ttgtgacagg ttgacccgtg
    16261 tgatcgacga gcccgtgtcc gcgcgcacca gtgccgcgct gccggcgttg agtaaggccg
    16321 agcgacgcgt cggtcgggcc ctgctcgccg actaccccag cgccggcctg gccagcgccg
    16381 ccacgttggc cgagcgcgcc gaggtcagcc cgcccaccgt gctgcggttt gcgcagtcac
    16441 tgggctacga gggcttcacc gatctgcagg tggcgctgcg cgcggaactg tcggcccgat
    16501 caagcggccc gatcgcgcgg ttgcctgatg cgccggcggc cggcagcatt ctcgagcgac
    16561 tgctgcagca ggcgcgcgcc caaaacgacc gtgccgcaga gactttggct cagctgcccg
    16621 catcagcgtt ggaggccgca gtgaccctcc tggcggacac caggcgggcg gtgtatctgc
    16681 acggtgggcg cttttctcac ttgctggcca ttcacctggc ggcgcatctc gagcagctgc
    16741 gcccaggaat acgagtgctc gacgacccca gcggccgcga tctcggggtg atgctcgaga
    16801 tgtctcgtac cgacgtggtc gtgctgttcg actaccaccg ctatcagcga agcgccgccg
    16861 aactcgcgtc atgggtgcac cgtgccggag cgacggtgtt gttgatcacc gatgacttgg
    16921 cctgcccggt ggctcccgac gccgaagtcg tgctggccgc gtcgagcacc gtgggcacca
    16981 cttaccagag catggccgcc ggattcctgc tcaccgaact cctcattccg ctcgtgatgg
    17041 acgctatcgg ggaacccgcg cgcactcgga tggcgctgtg ggaggagcaa ctccgcgccg
    17101 aactactgcc ctgacgagca caaatccacg ctcacgacac ccgcagggtc cctattgctt
    17161 actctggagt aatggccgga ggcaccaagc gtttgccgcg cgccgtgcgc gaacagcaga
    17221 tgctcgacgc cgcggtgcaa atgttctcgg tcaacggcta tcacgagacg tcgatggacg
    17281 cgatcgcggc cgaggcccag atctccaaac cgatgctgta tttgtattac ggctcgaagg
    17341 aggagctgtt cggggcgtgc cttgatcgcg agctgacgcg attcgtcgac gacgtgcgca
    17401 ccaagatcga cttctcgcag agcccaaaag agctgctgcg caacgccgtt ctcgcgttcc
    17461 tgacctacat cgacgcgaat cgggcgtcgt ggatggtgct ctacacccag gccaccagct
    17521 cgcaggcgtt cgcgcacacg gtgcgtgagg gtcgcgagcg catcatcgat ctggtgggca
    17581 ggctgctgcg gtcgggcacc cgccatcccg agccggacac cgacttcgac atgatggccg
    17641 tggcgttggt gggtgctggc gaagccgttg ctacccgggt cagcaccggc gatgccgatg
    17701 tcgacgaggc cgccgagttg atgatcaacc tgttctggcg tggcctgaag ggcacgccgt
    17761 cggataagga tgcccaggcg gtggcgtcca actaggctcg ccggacagtc gaggcgtggc
    17821 cttcggctgg aagaggtgca gtcaatggct gaaacaacga cgccgtctgg tcgtctggtg
    17881 tggcgcagtc tgggcatgtt gcgggcggtg cgaggcctga tcgtcgtcct aatccccctc
    17941 ggcgtcgttg cgtcggcgtt gccgtatgtc acggctgctg cctttgggcc catgatgcag
    18001 gtcgtcgccg acgccggggc gcgtggaaat ctcagtgggg tatgggattt gaaggggcct
    18061 ctcgtcgctc gtgaggacgg cttgcttcgg tcgcttgcgg gccctgtccc gttcgcggtg
    18121 ctccttgcga catggggaac ctcgctgttg ttgacccagt tgatgtactt gatcaacgcc
    18181 tcggtcaggg tgaaggctca acgggtactc gtcaccgaca tccggcagcg ggtacacgat
    18241 cacatccaaa cgctttcact ggacttcttc accgggtcgg gcagcggcgc gctgataccg
    18301 cgcgtgacga cggaatccgc tggcgtgcaa cgccttctga tggactgtct gctggcgcct
    18361 ctcatcgacg ccgtcgtgct ggctgtggcc atcgcctatc tgctggccct ctcgtggcag
    18421 atgacgatcg ccgcgctggc ccttacaccg gttgccttgc tcacgctgag attcgcaggg
    18481 cgcaatctcc aggcggtgat acaacgcgtg atgaacgcgg accgcgtgat ggccgcggag
    18541 ctcgagcaga cggtgaacgg gatcgcagag atccaaacgt tcaatgcgca gcccatacgc
    18601 agcaagcgat ttcaggtcgc ctcggagaac gctgcgaaga gcgacgcgtc gaccgtggtc
    18661 tggatgcagg ccacggctaa cggatcgcaa gtgttcatcg cgctcagtat tgtcgtggcg
    18721 ctcctggttg gcatcgggtt cagtgcgagc tttggtctca cgttcgccgg cttgatggtg
    18781 ttcgcaggaa tggtcccgac gatgttcggt gcggcgcaac gggtcatggg cgcgtacacc
    18841 acctaccagt cggtgctgcc gagtgcggca tcgacgtacg aactgctgga cactcgcccg
    18901 tcggttgagg accgagccga cgctatcccg ctcggcgatg tgcacggcaa cctggtattc
    18961 gaggacgtgg cctttggtta cacaccggga caaaacgtcc tcgacggtct gtcgttctcg
    19021 ataacagaag gcgagacggt ggccctggtg ggtggcatcg gatcgggaaa gtcgacggtg
    19081 ttcaacctcg tgctgcgctt cctggacccg cagcggggcc gcatactgct ggacggccac
    19141 gacatcactg cggtcaccat ggcctcactg cgtgagcagg tatcgaagct cgcccagttc
    19201 cccttcttca ccaaggacac gatccgcgag aacattcggt tggcccgacc ggacgccagc
    19261 gacatcgaca tcgagaaggc gtgcgaggac gcccacgtcc actccgtcat caccgatccg
    19321 gcgaagttgc gcgacggcta cgacaccgtg gtggacgtgc aagtgccatc tggaggacag
    19381 aaacgactga tcgcactggc gcgctgtttg ttgcgcaagc ccgaggtgtt gctgctcgac
    19441 gagccgaccg aaaacctcga cgccgaccag cgggcgaggc tcaaaggagt gatccgcggc
    19501 tatgcccgcg atcgcacctg catggtgatc agtcacgaca tggacttcat tgccgcggtt
    19561 gcggatcgaa ttctggtcct cgagcacggc cgcatcgcgc aggtgggcga ccaccacacg
    19621 ttgatggccg aaggtggcct ctacaagcga ctgtacgagg cgcagaacgt cgaccccgat
    19681 ctcgtctatc cgagcggcta ggctctcgcc cgatgaacgt catcacgatg ggggcccaca
    19741 tactcgatgt cctcgtgcgt ccggtcaccg acattccacc cggacaggaa acggcgttgg
    19801 tcgagcagat acggatgacg gtggcgggca ccgccgcagg gacggcgctg acgttcgcca
    19861 aactcggcgc gacggtgcaa acggccgggg cgatcgggac cgatcccgcg ggcgacctac
    19921 tcctgtctct gcttggccgc gccggcatcg acaccagcat cgtggtgcgc aaacccgatg
    19981 tgccaacgtc gatgtcggta ctgccgatcc ggccgaacgg cgaacggccg gccctgcacc
    20041 tgctcggggc caacctgtcc tacacgctgg acgacgtgga ctgggacgcc gtcgccgccg
    20101 cggaccacat ccatctcggc ggtacggaga tgctcggtcc tgacttcgcc gcgcgaatcc
    20161 tgaagcacgc caaggagaat ggagtcacga catctgtcga cctgatcgcg cccggcggta
    20221 tgggcacatt cgacctcatc gcgccggcga tggcctacac cgactacctg ctgccaaacg
    20281 aggaccaagt cctcggtttc accggagcca cggacctcga tagtgggagc cggaagctgc
    20341 tcgacgcggg cgccgggctg ctggccatca cgtgtggcgc cgacggagca tttgtcgtcg
    20401 acgatgaagg gaagcaacag gttccggcgt ttaccgtcaa cgtggtggat accaccggct
    20461 gcggtgacgc attctccgcc ggcttcgtct atgccacggg tatcggccgg ccgccccgcg
    20521 acgccgcggt cctcggcaat gcggtcgcgg cgctcgtcgc acagggtctg ggcagcgacc
    20581 acggtgagtt cgacctcgcg gccgccgacg cattcgcagc cagccaagcc gtaggaaacc
    20641 cgcgcgcaag cggatcagac ccggccgagc ggggttagtt gagggatctc attccaggtc
    20701 ggcgagggcc ttgcgggcgg cttctagttc ggcttcgagg gcggcgacct tggcggcctg
    20761 ctgagagcga gcctcttcga tgacctcgtc gatcggggtg gagaggtcct cgtgcagttc
    20821 cttggccgcg cgggaaacgg cggcggccgc gaccgtgagg ttgcgggcca ggtaggtggt
    20881 gccctgcttg agctcggcgc gccattcgcc gtcggcggta ccggtgaccg tgagggtcag
    20941 ctcgagtgtc ttggtcttct tgccggccgc cttcttggcc tgaaccttct tcggcttctc
    21001 ctcgacagaa gcggcatcgg cggacgtgtc gggcgcaggc gaaatgctct cgacagaagg
    21061 aaccaagacg tcgggagcgt cggagtcggt ggacggggct tctcgtggca gagtgtctac
    21121 agtcatcgtt gcggcgatct ccttcggaac ttcgcccgca gtgagcgggc tggcctaggt
    21181 tattagaaca catgttcgat gcccgggcca acttaccact cacggctcaa agaaagacct
    21241 cgccggcctt gggctttcgg actcgtggca tcgcctgtcc ctcgaggttc gctaaaaccg
    21301 ctgcggcgct gaacattcca tgggcgataa ccgtgggaaa accgaacaac ttcgcggcga
    21361 tcgggttggt gtgggtcgga ttgtgatccc cgccgaccga cgcgttcgcc aagaccacgg
    21421 cgctcccggg atagtccgcg aattgcccgc gttgcgagcg acaaaccgac acgcgagctc
    21481 cctgcctcac ctgcggacga tggtggacca ccagcccacc tcgcggcttc caagatgcag
    21541 ggccgtgacg actgcggcca gcggtggact gaccgcactc tgatttagtc agattgccgc
    21601 ccgattctga acccaaacgt caagcccagg atgcgcaccg taccaaccaa atccacggtg
    21661 tttgtcgttt cagccggtgc tcgcgggacg gtgggacggc gctgggttgc gcggttgtag
    21721 cgcgcagctt ggcgatcata gcgcgcccgg cgctgggggt cgcgcagcaa tgcgtaggcg
    21781 gcgaaaaccc gccgtagttg ctcatcggcg ggcgccggcg tcgactctgg tgtggttctg
    21841 gtgtccgggt gaaaggctcg tacttgcgtg cggtacgcat gcgtgatctc gtctggggtc
    21901 gccgttggtg acaaccccag cacggcgtag gggtcaaatc gctggcgcgg cacgttgcac
    21961 tcctcagagt tggctccttg cgagaccata tgacgctctg gcggcagaac gtgtacgtca
    22021 gtgttctacc cgtttattga cacaatcgaa acattcgagt gaatcgcgag tggccggtgc
    22081 gcgcatatgc gcccgatgtt gtcgctccct acgcgggcaa aacagaaagt gccttccggc
    22141 gagcgcgaac ctacagcgac gtcaccgtgc ccgtgaggtg cgggtcgccc ttcgacaggt
    22201 tacgcagcgt gagctgccac ccatcggaga tgcggtcgac gtacagaccc gcccgcgcag
    22261 gcagcacgac cggcttggcg aacttcaccg aatacttgac ggcgtctggg agctggccct
    22321 cgacgtttgc caaaaccgca gcggcggtga acattccgtg cgcgatcacg gtcggaaaac
    22381 caaacagctt ggctgcgacc gggttggtgt gaatcggatt gtgatccccg ccgaccgacg
    22441 cgtaatgccg gatctggccg ggcgtgatgc gcagcactgc atttggcggt cccggcttcg
    22501 gctgcttctg cggcggtggc ttcggctcgt cggacaggct ggttttctgc tgatgcagaa
    22561 acgtcgtcac ctgatgccac gcggtctcat tgccgacatt cacgtcggtg acgatgtcga
    22621 ccagcagccc gcgccggtgc tcccgcaggt tctcggcgtg agtccggacc gacaccgtgt
    22681 cggtcaccag gatcggccgg tactgcgtga tgtggttctc gatgtgcacc gaacccattg
    22741 cggcgaacgg aaaatcaaac cccgtgacaa gcgacatcac cgtcgggaac gtcagcgcga
    22801 acggatacgt cagcggcact gcatcgccga accgcaaccc agtcacattg gcgtacgccg
    22861 ctacgttcgc ggggtcgatc gccaactcgt cgacggtgag cgtgcggtcg ggcaatgtat
    22921 cgccgcgcgg aacgaacggc agcgccccgg ccgccgcgcg caccaaattc atcaaccccg
    22981 acggttgcgt cacgctacgc ccccagccat gcctgtccgc atacccgaat cgtattgcct
    23041 gtcacggcgt tcgacgccgg gctggcgaaa tacgcgatgg tttcggcgac gtcgaccggc
    23101 tggccgccct gatacagcga gttcagccgt cggcccacct cacgtgtggc cagcggaatc
    23161 gcctcggtca tcttcgtctc gatgaagccc ggcgcgaccg cgttgaccgt gatgcccttg
    23221 tcggcgtacg cctccgacag cgcgtccgtc agcccgatca tccccgcctt ggtggctgcg
    23281 tagttggtct gcccgcggtt gcccgcgatg cccgccatcg acgacaggcc gacgacccgg
    23341 ccgccctcgc caaggctccc gttgtcgatc aggccctcgg taagccgaag cggtgcaagc
    23401 aaattcactg cgacgaccga atcccaccgg gactcgtcca tattggccag cagcttgtcg
    23461 cgggtgatac ctgcgttgtt gacgaggatg tcggcgcgac cgccgtgcac ctcgcgcagg
    23521 tgctcggtga tcttgtcgac ggcgtcctcc gccgtgacat caagcgtcaa cgccgtgccg
    23581 cccaccttcg atgccgtcac acccagcggc tcagcagcgc cttctatgtc gatgcacacc
    23641 accttggcgc catcgcgtga aaacacctcg gcaatcgtcg ccccgatgcc gcgggccgcg
    23701 cccgtcacga tcgcgacctt gccgtccagc ggcttgtccc aatccgcggg cggcgccgag
    23761 tccgccgcgc cgacgcggaa gacctgcccg tcgacgtacg ccgacttgcc ggacaggatg
    23821 aaccgcaccg tcgactccag gccggttgcc gcgggcttgg catcgggcga taggtgcacc
    23881 aggttcaccg tcgcgccgcg ccgcagctcc ttggcgaggg agcgggtgaa cccctcgagc
    23941 gcccgctgcg cgatccgttc gtgcccgctg atttcgtcgg gcgtcgtgcc gatcaccacg
    24001 atgcgacccg aaggcccgag gttgcgcagc agcggagtga agaacttgta caggcccttc
    24061 aggcccgccg gttcggtgat gccggtggcg tcgaacacca gcccgccgaa cgaatcggcc
    24121 caacggccgc cgaggttgtt ggacaccacg tcgtagtcct cggcaagcgc agtgcgcagc
    24181 ggctcgacga cgcggccttc accgccgatc agcagcgtgc ccgccagcgg ctgatcgccg
    24241 ggcttgtagc gccgcagtgt ctccggctgc gggatgccga gctgcttggc gaggaacgat
    24301 ccgggcgtcg agtggacgat ttgtgagtac agatcggaag ccatagccct aacttactcc
    24361 agagtaagaa ccgtgagtag gatgggcccc atggccaacc aatcgagtcg ccgcgtagct
    24421 gttcttggcg gcaacaggat cccgttcgca cgatcggatg gcgcctacgc aaacgcctcc
    24481 aaccaggaca tgttcaccgc ggcgctgggc gggctgatcg accgcttcaa cctcgagggc
    24541 gaaaagctcg gcgcggtgat cggcggcgcg gtgctcaagc acagccgcga cttcaatctg
    24601 atgcgtgagt gcgtgctcgg cagctcgctg tcgtcctaca caccggcgtt cgacattcag
    24661 caggcgtgcg gcaccggact gcaggcgacc atcgcggccg ccgacggcgt cgcagccggg
    24721 cgctatgacg tggccgccgc cggcggggtg gacaccgcct ccgacgctcc gatcgccttc
    24781 ggcgacgacc tgcgccgcgt gctgctcggg ctgcgccggt cgaagtccaa cgtcgagcgg
    24841 ctcaagttgg tgggcaaact gcccgcatcg ctgggagtcc agatccccgt gaacagtgag
    24901 ccgcgcacgg gcctgtcgat gggcgagcac caggcgatca ccgccaagca gatgggcatc
    24961 aagcgggtcg accaggacga gctggccgcg gccagccatc gcaacatggc cgccgcctac
    25021 gaccgcggct tcttcgacga tctcgtcacc ccgttcctcg ggctgtatcg cgacaacaac
    25081 ctgcgggccg actcgtcgcc cgagaagctg gcgtcgctca ggccggtttt cggggtgaag
    25141 gccggtgacg cgaccatgac ggccggcaac tcgaccccgc tgacggatgg cgcgtccgtg
    25201 tcgctgcttg cgacgcccga gtgggcgtcg gcgcacggca tcgaaccgct ggcgtactac
    25261 gtcgacgccg agacggccgc ggtcgactac gtcaacggca aggacggcct gctgatggcc
    25321 cccacctatg cggtgccgcg gctgcttgcc cgaaacgggt tgagcctggg cgacttcgac
    25381 ttctacgaaa tccacgaggc gttcgcgtcg gtggtgctcg cgcatctgca ggcctgggag
    25441 tccgagcagt actgcaagga gcggctcggg cttgacgcgg cgctcggctc gatcgaccgg
    25501 tccaagctga atgtcaacgg ctcgtcgctg gccgcgggtc acccgttcgc cgcgaccggc
    25561 ggcaggatcg tggcgcagct ggccaagcaa ctggccgaga agaagaagga gaccggccag
    25621 cccgtgcgcg gcctcatttc gatctgtgca gcgggtggtc agggcgtcgc ggccatcttg
    25681 gaggcgtaga aaaaaacttt cgcgggcgct gtaacgcgcc cgcgggggtc gtcgatacac
    25741 ccggtagctt cgtgttgccg tctgaccccc cgacccgacg gcaacacggg gctcttattt
    25801 tttcgcccgg gatgcttcga tgaggtcatg caaatcagcc tcagcctcgt cggactactc
    25861 agcgactcga ccggtgcttc gccggtcgac gggaccataa agaacctcgc ccggcttcgc
    25921 gacgagggct tccgcagagt ttggatggcg cagctgccct acgacccaga tctgctgacg
    25981 atcctcgccg tcgcactcca cgaggtcgac accatcgagg tggggtccgg cgtggtaccg
    26041 atccaggtcc agcatccgac gcagttggcg cagcgggcgc tgacgcttaa tgcgatcgca
    26101 ggcgggcggt tcagcctcgg catcggcatg agccacaaga tggtcaccga acagatgtgg
    26161 ggtatctcct acgagaagcc gctgcgccgc atgcgcgagt acctcgacgg cctgctgccc
    26221 ctgttgtccg ggcaggccgc cgacgcggtc ggggagacgg tgacgacccg cgggtcgctg
    26281 cagattccgg gtgcaccgac gcccgacgtc tacatcgccg cgctgggccc gcagatgctg
    26341 cggctggcgg gacgccgcac ggcgggcacg ctgacgtgga tgaccgggcc caagaccctg
    26401 gccgagcacg tcggcccggc gctgcgtgag gccgccgccg aggccggtcg cgaggaatcg
    26461 gcggtccggg tcgctgcctc gctgccggtg agcgtcaccg acgatatcga cgccgcccgc
    26521 gcccgtgccg ccgaagagtt cgcgatctac ggccagctgc cgtcgtaccg cgc
//
LOCUS       DASRJA010000761        23571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2307146,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000761 DASRJA010000000
VERSION     DASRJA010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2307146"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFC01_34410"
     CDS             complement(<1..233)
                     /locus_tag="VFC01_34410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84750.1"
                     /translation="MPPPRLPRGAAPRRRAGRPELSDAAVVSRSWGMAFATLISRITG
                     FTRIVLLAAILGAALSSAFSVANQLPNLIAALVL"
     gene            complement(332..2782)
                     /locus_tag="VFC01_34415"
     CDS             complement(332..2782)
                     /locus_tag="VFC01_34415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HZN84751.1"
                     /translation="MKIAAVVARLLAALALLTLIALPAAVPHAAAGEPGAVPFLQVRI
                     DRIAPEVVTTTSEPVVTVSGSILNVGDRPVRDVMVRLEHAAAVTSSAGLRTNLSGNVD
                     QYEPVADFITVAPELLRGQDVPFNLVYPLRSAELPSLNIGEPGVYPVMVNVNGTPDYG
                     APARLDDARFLLPVLGVPPEPAADSAADTLTSAVPPDTSKPVGLTMLWPLADRPRLAA
                     GAPGGTTPVRFIDDDLAASLAPGGRLDVLLSAVDFATSPQVDQGGEVRNAMCLAVDPD
                     LLVTVNAMTGGYVVNDAPDAGPGTPTHPGAGQDAAVGWLNRLKALAQRICVAPTTYSH
                     ADLDALQRVGDPGLSATATKGAADIVDQILGITSTRGATLVGDGPLTGPAVELLSAQG
                     PTVAIGAANAAAQDSATGEPATADVRPVRYTPHVVAAPFDPTVGAALAGAGTEPASPS
                     YLDPSLDIPVKHDSAVARRQDALASLLWRGLQPDTEPRTQVLMPPLAWNLQPDDAQAI
                     LTALASTIHAGLAVPRPLTAVIAEANAVPPQDLAPLPDGELGNPRGRFDDGVVSGIAG
                     VTGRLWGLTAALTTDERTGLTGPQYTAPLREDMLRALSQSVPPDARNGLAQQRLTTVG
                     RTVDDLFGAVTIVNPGGSYTLATEHSPLPLALRNNLPVPIRVRLQIEAPPGMSVTDMG
                     EIMLPPGYLPLKVPIEVHFTQRVAVDVALRTADGQPLGEPVRLSVHSNAYGKVLFFIT
                     LTGGAVLALLVGRRLWHRFRGQPDRADLDRPDPIDVAIAYGKDVDEPRVMSRSAGGEG
                     RAGSAGWEGRAGSAGGDQ"
     gene            complement(2779..3540)
                     /locus_tag="VFC01_34420"
     CDS             complement(2779..3540)
                     /locus_tag="VFC01_34420"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZN84752.1"
                     /translation="MSDGEQAKPRRRRGRRRGRRAAGPPESTEHRNNSNPVHAKKDQP
                     NGQNKPQKSRPRRPADRLRTVHETSAGGLVIDGIDGPKETQVAALIGRIDRRGRMLWS
                     LPKGHIELGETAEQTAIREVAEETGIQGSVLAALGSIDYWFVTEGRRVHKTVHHYLMR
                     FLGGELSDEDLEVSEVAWVPLRELPSRLAYADERRLAEVAGELIDKLHADGPASLPPL
                     PRTSPRRHAQTHSHTRNRRPDEQPSRRTNGCGQGP"
     gene            3626..5074
                     /locus_tag="VFC01_34425"
     CDS             3626..5074
                     /locus_tag="VFC01_34425"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HZN84753.1"
                     /translation="MPDSAADAELLATALVALNEHGDVLRELGRLFADAGHELYLVGG
                     SVRDALLGRLGNDLDFTTDARPEQMQKMLRSWADSLWDTGIEFGTLGVGKDGDRLEIT
                     TFRADTYDQVSRNPEVRFGDRLDDDLVRRDFTVNAMAVRVTADGPGEFLDPLGGLAAL
                     RARVLDTPSAPQVSFGDDPLRMLRAARFVSQLEFAVAPRVVSALLEMAPQLGRITAER
                     VAAELDKLLLGADPVAGVDLMVQTGLGDVVLPEVGAMRMAIDEHHQHKDVYWHSLTVL
                     RQAIGLEDDGPDLVLRWAALLHDIGKPATRKHEADGGVSFHHHEVVGAKMVRKRMRAL
                     RYSKQMIDDVSQLVYLHLRFHGYGGGQWTDSAVRRYVTDAGPLLPKLHRLVRADCTTR
                     NQRRAARLQANYDDLERRIDELAAKEDLARVRPDLDGNEIMQILGIPAGPEVGEAWRY
                     LKELRLDRGPLSHDEAVDELMKWWNSPERGNV"
     gene            5081..5569
                     /locus_tag="VFC01_34430"
     CDS             5081..5569
                     /locus_tag="VFC01_34430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HZN84754.1"
                     /translation="MEYCLGDADGSATMWTAAPDVDVDGDGSLDAVGLDFDGDGLLDD
                     AMTDTDGDGLADHAVLDFASDATFFTDDGTGTWAVAVDRAGQLRWFGLDGVEHTGGPV
                     VDVDADGQTDDRLTDTDGNGLADRALSGDVAYVDADGDGRWDVKLADADGDGKADAAT
                     EL"
     gene            complement(5566..6342)
                     /locus_tag="VFC01_34435"
     CDS             complement(5566..6342)
                     /locus_tag="VFC01_34435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03084 family metal-binding protein"
                     /protein_id="HZN84755.1"
                     /translation="MSTAEPMVADLRAESDDLDDLVADLPADRWAELTPAPGWTIAHQ
                     IAHLLWTDRVALTAVTDEAGFADVLAEAAKDPTGFVDAGAEELAAPPPEQLLADWRQT
                     RGRLHDELLAVADGRKLPWFGPPMSAASMATARLMETWAHGLDVADALAVKRPATARL
                     KSIAHIGVRTRDFAFSINGLTPPAEPFRVELRAPDGSTWSWGPDDAAVRVIGSAEDFC
                     MLVTQRRPRAALDVRAVGDDAEKWLGIAQAFAGPPGPGRD"
     gene            complement(6353..7636)
                     /locus_tag="VFC01_34440"
     CDS             complement(6353..7636)
                     /locus_tag="VFC01_34440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN84756.1"
                     /translation="MADIRAPIAVWRSVRALPEFWRLLELRTVSQFGDGLFQAGLAGG
                     LLFSTDRAAGPWEIAGSFAALYLPYSVLGPFAGALLDRWDRRLVLIGANIGRLVLVLG
                     IAALLAVGAGDPLILFGALIVNGFTRFVSSGLSAALPDVVAREQVVTMNAVATATGAA
                     AAFLGAIFMLVPRSLFGADDTGAAAIIFIVTVPVALALLLSVRFPPHILGPHESKRSI
                     HGSVMYAVATGWLHGARTVLAVPTVAATLSGLAAHRMVLGINTLLVLVMVRHTDTKEV
                     AGFGTSVMFLAAVGLGSFLATALTPSIVRRWGRYAAPNGALAFAAAVQLCGSGLQLPV
                     MVLCGFLLGAAGQVVKLCADTAMQIDVDDALRGHVFTVQDALFWMAFIVAMTLAATVI
                     PADGHEPALVFAGSVVYLAGLAVHASVGRRVQSPG"
     gene            7755..8360
                     /locus_tag="VFC01_34445"
     CDS             7755..8360
                     /locus_tag="VFC01_34445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HZN84757.1"
                     /translation="MAHSEDPEDFLAPAAHRVRAGTLLLANTDLLEPTFRRSVIYIVE
                     HNDGGTLGVVLNRPSETAVYNVLPQWAKLATKPKTMFIGGPVKRDAALCLGTLRVGLE
                     ADGVPGLRHVQGRIVMVDLDADPDAIAPMVEGVRIFAGYSGWTIGQLEGEIERDDWIV
                     LSALPSDVLVEARVDLWSRVLRRQPLPMSLLASHPIDISRN"
     gene            complement(8357..9326)
                     /locus_tag="VFC01_34450"
                     /pseudo
     CDS             complement(8357..9326)
                     /locus_tag="VFC01_34450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010839957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(9333..9707)
                     /locus_tag="VFC01_34455"
     CDS             complement(9333..9707)
                     /locus_tag="VFC01_34455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84758.1"
                     /translation="MLIAAVLCLCAAVATAGLGVWLLTRPRSGDFVRQVLRGVAPTQL
                     AAAVMLAAGGAVALSSRPQTGLLVVVVCVIGAISTVAAGCWQSAKVAAAAAANSAGAR
                     AQTAPAAGGCGGACATCTLSCN"
     gene            complement(9788..10660)
                     /locus_tag="VFC01_34460"
     CDS             complement(9788..10660)
                     /locus_tag="VFC01_34460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqN/LpqT family lipoprotein"
                     /protein_id="HZN84759.1"
                     /translation="MNEIARRWRILAGGTAAGVAAVVGLAGGTASAEPVFPQPPLPGP
                     ASVTQTVTVPTAPQALGQPGVTPATPAPSPPPVTITPASSGTLDDFFKAKAVKLEPQN
                     SRDFKALNIVLPMPKGWAQVPDPNVPDAFAVIADRVGGNGLYSTNAQVKVYKLIGDFD
                     PKEAISHGFIDSQLLPAWRATDGSLADFGGMPSSFIEGTYRENSMTLNTSRRHVIATA
                     GPDKFLVSLAVTTSVDQVVASADSTDAIVNGFKVSLPGPPTPTPLPPAPGLPQLPASP
                     SSLGAQPQPLGQTG"
     gene            10849..13689
                     /gene="leuS"
                     /locus_tag="VFC01_34465"
     CDS             10849..13689
                     /gene="leuS"
                     /locus_tag="VFC01_34465"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZN84760.1"
                     /translation="MTEPEVDTPQHRYTAELAGKIEHAWQQIWAESGTFNVPNPVGSL
                     APPDDAPVPADKMFVLDMFPYPSGEGLHVGHPLGYIATDVYARYYRMTGRNVLHALGF
                     DSFGLPAEQYAIQTGTHPRIRTEANIVNFRRQLGRLGFGHDARRSFSTTDVDYYKWTQ
                     WIFLQIYNAWFDPAANKARPITELVAEFESGARRLDDGRQWSELSAGDRADVIDSYRL
                     VYRADSMVNWCPGLGTVLANEEVTSDGRSERGNFPVFRKRLRQWMMRITAYSDRLLDD
                     LDLLDWPEKVKTMQRNWIGRSTGASVLFGTAAGDIEVFTTRPDTLFGATYMVLAPEHE
                     LVDKLTASEWPAGVDERWTFGSATPAEAVAAYRATIAAKSDLERQENKDKTGVFLGGY
                     ATNPVNGQQIPVFIADYVLLGYGTGAIMAVPGGDQRDWDFAHAFGLPIVETVAGGDIS
                     QGAYGGDGEVVNSDYLNGLSVAAAKEAITRRLEADGRGRARIEYKLRDWLFARQRYWG
                     EPFPIVYDADGRAYPLPESALPVELPDIPDYAPVQFDPDDADSEPSPPLGKATDWVHV
                     ELDLGDGLKPYSRDTNVMPQWAASSWYELRYTDPHNSEELCAKENEAYWMGPRPAEHG
                     AKDPGGVDLYVGGVEHAVLHLLYARFWHKVLYDLGHVSSKEPYRRLVNQGYIQAFAYT
                     DSRGAYVPAAEVTERDGTFYWTGPDGEIEVNQEFGKIGKSLKNSVSPDEICDNYGADT
                     LRVYEMSMGPLEASRPWATKDVVGAYRFLQRVWRLVVDEQTGITHPAEHEALDDETLR
                     LLHRTIAGVADDYASLRNNTAAAKLIEYTNHLTKQGVRARAALEPLVLMVAPLAPHLA
                     EELWQRLGHDKSLAHGPFPVADPQYLVEDTVEYPVQVNGKVRGHVTVPADADADTMEA
                     AALADEKVQAFLNGAAPKKVIVVAGRLVNIVV"
     gene            complement(13743..14159)
                     /locus_tag="VFC01_34470"
     CDS             complement(13743..14159)
                     /locus_tag="VFC01_34470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZN84761.1"
                     /translation="MSSIKDTAERFFEACETGKGWSGCEPYCHPGATFAAQTDALADV
                     TTLEAYSEWMKGLMTILPDGNAEVRSFAVDEERKNVAAFGVFRGTHTGDGGPVPPTGK
                     SAEADYVYVMDFDGDKIRHMTKIWNDGITMRQLGWA"
     gene            complement(14210..14875)
                     /locus_tag="VFC01_34475"
     CDS             complement(14210..14875)
                     /locus_tag="VFC01_34475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN84762.1"
                     /translation="MPTAMITGAGRGLGSALAEVLAPTHTLFLAGRPSSRLDEVAARF
                     GATTWPVDLADTDAIRAVVEPIVELDVLIHNAGVAYPGRVAESSVDEWRKSMEVNVIG
                     AVALTLALLPALRAAGGHVVFINSGAGINASPGLASYTASKFALRGFADSLRDDEPSL
                     RVTSIHSGRIATEMQEGLIAYEGKDYDPSQFLSPETVAKVVADTINAPRDAQINEVIV
                     RPR"
     gene            14929..16269
                     /locus_tag="VFC01_34480"
     CDS             14929..16269
                     /locus_tag="VFC01_34480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein"
                     /protein_id="HZN84763.1"
                     /translation="MPPDHSFAPTQLAARAAYLLRGNDLGAMTTAAPLLYPHMWSWDA
                     AFVSIGLAPLSVERAVVELDTLLSAQWTNGMIPHIVFANGVDGYFPGPARWATSALAA
                     NAPRTRHTSGITQPPVHAIAVQRILDHARTRGRSTRAVAEAFLDRRWADLVRWHRWLA
                     EARDPKSRGRVTLYHGWESGMDNSPRWDSAYANVVPGNVPEYQREDNTIVTDASQRPS
                     DLEYDRYLWLLEEMKSARYDDELLPKVMSFAVEDVFVSAILSVACTVLAEIGEDYKRP
                     HADVKDLYAWAERFRRGVVETTDERTGAAKDFDLRADRWVATETVAQFAPLLCGGLSH
                     DKERTLLRLLEGPRFCGHPDLRYTLIPSTSPVSRDFRQREYWRGPVWPVMTWLFSWCF
                     ARRGWAERALMLKREGLRQASDGTFAEYYEPFTGEPLGSMQQSWTAAAVLDWLG"
     gene            complement(16273..16716)
                     /locus_tag="VFC01_34485"
     CDS             complement(16273..16716)
                     /locus_tag="VFC01_34485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN84764.1"
                     /translation="MVETESQVGELAGELQRVLSKVFSVLRRGDTNRTDAGDLTLAQL
                     SILLTLLDQGPIRMTELAAHERVRTPTTTVAIRRLEKLGLVKRSRDPSDLRAVLVDIT
                     PKGLSQHREALAARRAHLATLMAKLSPEDLETLTKALVPLERLAE"
     gene            complement(16853..17467)
                     /locus_tag="VFC01_34490"
                     /pseudo
     CDS             complement(16853..17467)
                     /locus_tag="VFC01_34490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            complement(17555..18229)
                     /locus_tag="VFC01_34495"
     CDS             complement(17555..18229)
                     /locus_tag="VFC01_34495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN84765.1"
                     /translation="MPKKYGVKEKDLVVSHVVNLVLTGKLRSGDRIDRNEIAQELGLS
                     RVPIQEAVVQLEHDGVLSTQYHRGAYVERFDESVVREHHELYGLLSGIASARAAVDGL
                     PRILDTLGALLDVMRASKESRAFQETAWKYRRVINDEFAGPRLQAAIRASQTFIPRTF
                     WLAYLNNHDEILPFYEAETAAIQRRDPDGARAACAERAELMARIMLTELVRRGVFRPS
                     GASIAF"
     gene            complement(18281..19081)
                     /locus_tag="VFC01_34500"
     CDS             complement(18281..19081)
                     /locus_tag="VFC01_34500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN84766.1"
                     /translation="MSFPIRIGVQLQPQHSPQYSHIRDAVRRCEDIGVDVAFNWDHFF
                     PLYGDPDGPHYECWTMLGAWAEQTSRLEIGALVTCNSYRNPELLADMARTVDHISEGR
                     LILGIGSGWKQKDYDDYGYEFGTAGSRLDDLADAFPRITSRLAKLNPAPTRDIPVLIG
                     GQGEKKTLRLVAEHGDIWHGFTSLETYPAKAEILARHCADVGRDPATIERSAGVAGAN
                     LDALLEEAGAFVELGVTMLTVGVNGPDYDLTQAEALCRWRDRRQQNGN"
     gene            complement(19092..21161)
                     /locus_tag="VFC01_34505"
     CDS             complement(19092..21161)
                     /locus_tag="VFC01_34505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HZN84767.1"
                     /translation="MALVPLNLLVSHDGRSSRQHVTCRYKCGDACSKPAPNTSDNEYF
                     GDIVKAVSRRSMLQAGGIAVLAVGAGSALAACGQGEQPAATPTSSPAPAEPPPGMNFT
                     AVAPNKEDAVVIPEGYQQAVVISWGDPILPNAPKFDVRAQTAAAQRGQFGFNNDFAGL
                     LPVPGQKNRFLLVTNHEYVSPQFMFPGYDDAKPTREQFDIEIAAIGMGVVEVERTPEG
                     LKPVIGPYNRRITADTPFTLTGPAAGTDFVKTPADPAGRTVAGTFANCSGGVTPWGTV
                     LSGEENFHEYFGAPEGAPEPGPVDADRQDRYGVTLEPTELKWESFDPRFDVAKTPNEV
                     NRFGYVVEINPWDPNSKPVKHSALGRFKHEAANVYVTSDGTVVAYAGDDERFDYMYKF
                     VSAKKIQAGTDPVAMAHNMTILDEGTLYVAKLSSDIPADEIDGSGKLPKEGAFSGSGT
                     WIPLLRSGANGQGESLVDGVTAQEAAVFTRLAADKVGATKMDRPEDFEANPKTGRVYV
                     ALTNNDKRGAEGKAPVDAANPRNENKNGQILEITDNHAGTDFTWDLLLVCGDPAAADT
                     YFAGFDKTKVSPISCPDNVAFDSHGNLWISTDGNALKSNDGLFAVALEGPNRGETKQF
                     LTVPKGGETCGPVVTDDLVTVCVQHPGEGDDYTIDKPLSHWPEGGNGTARPSVVAVWK
                     ANGQIGV"
     gene            complement(21268..22173)
                     /locus_tag="VFC01_34510"
     CDS             complement(21268..22173)
                     /locus_tag="VFC01_34510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN84768.1"
                     /translation="MTNAEVSDDDLAGLDEFGLLHENAEQIGVGGPLPSVQRIESGSI
                     SALKWGDEPPQVVFLHGGGQNAHTWDTVIVGLGLPALAIDLPGHGRSAWREDGDYGPW
                     LNAETLRPLLREWAPNPRLVVGMSLGGLTALRIAASEPSLVPELVLVDVTPSAPERHE
                     QMTKAQMGTVALVQGDRTFPTFAAMLDVTVAAAPHRDRKSLRRGVFHNSKQLDDGTWT
                     WRYDSFRKGDGFDGLWDDVPAITMPTTLVRGANSYFVNDEDADAFAKAAPGFQRTHVV
                     ADSGHSVQGDQPAALVEILRGVLGA"
     gene            complement(22193..23026)
                     /locus_tag="VFC01_34515"
     CDS             complement(22193..23026)
                     /locus_tag="VFC01_34515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84769.1"
                     /translation="MQDQLRDMWGSVLAIAPKLVLFLIILIVGWIVAKLIAKAVDKIL
                     ERVGFDRAVERGGMRRAMERSNYDASTIVSKIVYYGLLLIVLNFAFGVFGPNPVSELI
                     AAVIAFLPRLFVALIIVVVAAAIAAAVRDIVSNALSGFSYGRALGTLASVFILGLGII
                     AALNQVGIALTVTLPVLVAVLATIGGILVVGVGGGLIKPMQARWEDYLTTAEREGRSI
                     RERRSSADAAPVGTVASERAPESDYKWAAEEPSGQPQRTTATAPSAKRPAHYSEGRPK
                     A"
     gene            complement(23186..>23571)
                     /locus_tag="VFC01_34520"
     CDS             complement(23186..>23571)
                     /locus_tag="VFC01_34520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HZN84770.1"
                     /translation="ERERLESKKISKTQAVTSNLQREFKTKDVHIGPSDHVGWLDDRK
                     WAYVRLEGRAFGDVPLNLEYKLEVWDSPNSAGVIIDAVRAAKIAKDRGIGGPVVAASA
                     YLMKSPPKQLPDDIARAQLEDYIKG"
BASE COUNT         3911 a         7806 c         8078 g         3776 t
ORIGIN      
        1 agcaccaggg cggcgatcag gttgggcagc tggttggcca ccgagaaggc cgacgacagc
       61 gcggcgccca ggatcgcggc cagcagcacg atgcgggtga agccggtgat cctgctgatc
      121 aacgtcgcga acgccatgcc ccacgaccgc gacaccaccg cggcgtcgga caattccggg
      181 cgtcccgcgc ggcgtcgcgg tgccgccccg cgggggagcc ggggcggggg catgcggcgg
      241 gtcggcggcg ggtaggggcg cggcggatag tgccgcgggg gggctctgtt gaacggcggt
      301 ggctgcggcg gaggcggccc gccagcggta ctcattggtc accgccggct gagccggcgc
      361 gaccttccca gccggctgag ccggcgcgac cttccccgcc cgcagaccgg ctcatgaccc
      421 gcggctcgtc gacatccttc ccgtaggcga tcgcgacgtc gatcgggtcc ggccggtcta
      481 ggtcggcgcg gtcgggctgg ccgcggaatc ggtgccagag tcgtcggccc accagcagcg
      541 ccagcacagc accgccggtc agcgtgatga agaacagcac cttgccgtag gcgttcgagt
      601 gcaccgagag ccgcaccggc tcgccgagcg gctggccgtc ggcggtgcgc agcgcgacgt
      661 ccaccgcgac acgctgcgtg aagtgcacct cgatcggcac cttcagcggc aggtagcccg
      721 gcggcagcat gatctcgccc atatccgtca ccgacattcc gggcggggcc tcgatctgta
      781 gccggacgcg gatcggcacc ggaaggttgt tgcgcagcgc gagcggcagc gggctgtgct
      841 cggtggccag tgtgtaggaa ccgcctgggt tgacgatggt gactgcgccg aacaggtcgt
      901 cgacggttcg gccgacggtt gtcagccgct gctgggccag cccgttgcgg gcgtccggcg
      961 gcaccgactg gctcagcgcg cgaagcatgt cttcacgcaa cggcgcggtg tactgcggcc
     1021 cggtgagccc ggtgcgctcg tcggtggtca gcgccgcagt gagaccccac agccgccctg
     1081 tcaccccggc gatgccggag acgacaccgt cgtcgaagcg gccgcgcgga ttgcccaact
     1141 cgccatcggg cagcggggca aggtcctgcg gcggcaccgc gttggcctcg gcgatcaccg
     1201 ccgtcaacgg ccgcggcacg gccagccccg catgaatggt ggaggccagc gcggtgagta
     1261 tcgcctgtgc gtcgtcgggc tgcaggttcc aggccagcgg cggcatcagg acctgggtgc
     1321 gcggttcggt gtcgggctgc aggccgcgcc acagcaatga ggccagcgca tcctggcggc
     1381 gggccaccgc tgaatcgtgt ttgaccggaa tgtccagcga gggatccaga tacgacggcg
     1441 acgccggttc ggtgcctgcg ccggccagcg cagcaccgac ggtcggatcg aacggcgcgg
     1501 cgacgacgtg cggcgtgtat cgcaccggcc ggacgtccgc ggtcgccggc tctccggtgg
     1561 cggagtcttg cgcggcggcg ttcgccgcgc ctatcgcgac cgtcggcccc tgcgccgaca
     1621 gcagctcgac ggcggggccg gtcagcggac catcgccgac gagggtggcg cctcgcgtcg
     1681 acgtgattcc caggatctgg tcgacgatgt cggcggcacc tttggtggca gtggcgctga
     1741 gccccgggtc gccgacccgc tgcagtgcgt cgaggtcggc gtgcgagtac gttgtcggcg
     1801 ccacacatat gcgctgcgcg agcgccttga gccgattgag ccagccgacc gccgcgtcct
     1861 gacccgcgcc cggatgtgtc ggggtgccag gcccggcgtc gggcgcgtcg ttgacgacgt
     1921 agccgcccgt catcgcgttg accgtgacaa gcaggtcagg atcgacggcc aggcacatcg
     1981 cgtttcgcac ctcgccgccc tggtcaacct gcggactggt cgcgaagtcg accgcagaca
     2041 acaggacgtc gagcctgccg ccgggggcga gcgaggccgc gaggtcgtcg tcgataaacc
     2101 gcacgggtgt ggtgccaccg ggcgcgcctg ccgccaaccg gggccggtcg gccagcggcc
     2161 acagcatggt cagtccaacc ggtttggacg tatcgggcgg caccgccgac gtcagcgtgt
     2221 cggcggccga gtcggccgcg ggctcgggcg gcacacccag tacaggaagc aggaagcggg
     2281 catcgtcgag gcgggcggga gctccgtagt cgggggtgcc gttgacgttg accatcaccg
     2341 ggtacacgcc cggttcgccg atgttcagcg acggcagttc cgctgatcgc agcggataga
     2401 cgaggttgaa cggaacatct tgtccgcgaa gcaattccgg tgcaacagtg atgaagtcgg
     2461 cgacgggctc gtattggtcg acgttgccgg agagattggt gcgaagcccg gccgacgacg
     2521 tgacggcggc ggcgtgctcg aggcgcacca tcacatcgcg gaccggtcga tcgccgacgt
     2581 tgagtatcga tccgctgacg gtgacgaccg gctcgctggt ggtggtgacc acctcggggg
     2641 cgatccggtc gatgcgcacc tgcaggaaag gcactgcccc cggctcgccc gcggcggcgt
     2701 ggggcacggc ggcaggaagc gcgatcaggg tcagcagggc gagggcggcc aacagccgcg
     2761 ccaccactgc cgctatcttc acggcccttg tccgcagccg ttcgtccggc gagatggctg
     2821 ttcgtcggga cgacggttgc gggtatgcga atgcgtctgg gcgtgccgtc gcggcgaggt
     2881 gcgcggcagc ggcggcagcg aggcgggccc gtctgcatgc agtttgtcga tcagctcgcc
     2941 ggcgacctcg gcgagccggc gttcgtcggc gtaggccagg cgcgacggca attcccgcag
     3001 cggcacccac gccacttcac tgacttcgag atcttcgtcg gagagttcgc cgccgaggaa
     3061 ccgcatcaaa tagtggtgca cggtcttgtg cacgcgacga ccctcggtga cgaaccagta
     3121 atcgatgctg ccgagcgcgg ccagcacact gccctggatg ccggtctcct cggcgacctc
     3181 ccggatcgcg gtttgttcag cggtttcacc cagctcgatg tggcccttgg gcagcgacca
     3241 cagcatccgt ccgcgccggt cgatgcgccc gatcagcgcg gcgacctgag tttccttggg
     3301 cccgtcgatg ccgtcgatca ccagcccgcc cgcggaggtt tcgtgcacgg tgcgcagccg
     3361 atcggcaggt ctgcgtggac gggatttctg cggtttgttc tgaccgttcg gctggtcctt
     3421 cttggcgtgc accggattgc tgttgttgcg gtgctcggtg gactcgggcg gaccggccgc
     3481 gcgtcggccg cggcgccgcc ctcggcgccg tcgtggtttg gcctgttcgc cgtccgacac
     3541 ccaagcgata gtagctgcca cgaagattcg ctcccgccgc actcaccggt cgtgacagag
     3601 ccgttgccga ttaggctgtc cgaacgtgcc cgattctgct gcagatgccg agctgcttgc
     3661 taccgcattg gtggcgttga acgagcacgg cgacgtcctg cgcgaactcg gccggttgtt
     3721 cgctgacgca ggtcacgagc tgtatctcgt cggcggaagt gtgcgcgacg cgctgctcgg
     3781 gcgcctgggt aacgacctgg acttcaccac cgacgcgcgg cccgagcaga tgcagaagat
     3841 gctgcggtcg tgggccgact cgctgtggga caccggcatc gaattcggca cgctcggcgt
     3901 cggtaaggac ggcgaccggc tggagatcac caccttccgc gccgacacct atgaccaggt
     3961 ttcgcgcaac ccggaagtgc ggttcggcga tcgcctcgac gacgatctgg tgcgccgtga
     4021 cttcacggtg aacgcgatgg cggtgcgcgt caccgccgac ggccccggcg aattccttga
     4081 tcccctaggc ggtttggcgg ccctgcgggc tcgcgtgctg gacacaccgt cggctccgca
     4141 ggtgtcgttc ggcgacgatc cgctgcggat gttgcgcgcg gcgcggttcg tgtcgcagct
     4201 cgagttcgcc gtcgcaccgc gcgtggtttc ggcgctgttg gagatggcgc cgcaactggg
     4261 tcgcatcacc gcggagcgcg tggccgccga actggacaag ctgctgttgg gtgcggaccc
     4321 ggtcgcgggc gtcgatctga tggtgcagac cggcctgggc gatgtggtgc tgccggaagt
     4381 cggtgcgatg cggatggcca tcgacgaaca ccaccaacac aaggacgtct actggcattc
     4441 gctgacggtg ctgcggcagg cgatcgggct ggaggacgac gggcccgatc tggtgctgcg
     4501 ctgggcggcg ctgctgcacg acatcggcaa gccggccacc cgcaagcacg aggccgacgg
     4561 cggggtgagt tttcatcacc acgaggtcgt cggcgccaag atggtccgca agcggatgcg
     4621 ggcactgcgc tactccaagc agatgatcga cgacgtgtcg cagctggtgt acctgcatct
     4681 gcggttccac gggtacggcg gtggccagtg gacggactcc gcggtgcgca ggtatgtcac
     4741 cgacgccggg ccgttgttgc cgaagctgca caggctggtg cgcgccgatt gcaccacccg
     4801 caatcagcgc cgcgcggccc ggctgcaggc caattacgac gacctggaac gccgcatcga
     4861 cgaattggcg gcgaaggagg atctcgcgcg agtgcggccg gatctcgacg gcaacgagat
     4921 catgcagatc ctcggcatcc cggccgggcc cgaggtgggg gaggcgtggc gatatctcaa
     4981 ggagctgcgg ctcgaccgcg ggccgttgag tcacgacgag gccgtcgacg agctgatgaa
     5041 gtggtggaac tcgccggagc gcggcaacgt ctaaaccgcc atggagtact gccttggcga
     5101 cgcggacggc tcggcgacga tgtggacggc cgccccggat gtcgacgtcg acggggatgg
     5161 ctcgctggac gccgtcggcc tggatttcga cggcgacggg ctgctggatg acgcgatgac
     5221 cgacacggac ggcgacggcc tcgccgacca cgcggtgctc gacttcgctt cggacgccac
     5281 gttcttcacc gacgacggca ccggcacgtg ggcggtagcg gtcgaccggg cgggtcaatt
     5341 gcggtggttc ggcttggacg gtgtcgagca caccggggga ccggtggtcg atgtcgacgc
     5401 cgacgggcag accgacgacc gcctgaccga taccgacgga aacgggctgg cggaccgcgc
     5461 gctgtccggt gacgtcgcct atgtcgacgc cgacggtgac ggccggtggg acgtcaagct
     5521 cgctgatgcg gatggcgacg gcaaggccga cgccgccacg gagctctagt cacggcccgg
     5581 gcccggcggg ccggcgaatg cctgcgcgat accgagccat ttctcggcgt catcgccgac
     5641 ggcacgcacg tctagggcag cccgcggcct acgctgcgtc accagcatgc agaagtcttc
     5701 ggccgaaccg atcacccgca cagctgcgtc gtccggcccc cacgaccacg tcgagccgtc
     5761 gggcgcgcgc aactccacac ggaacggctc cgcgggtgga gtgagcccgt tgatggagaa
     5821 cgcgaagtcg cgggtgcgca caccgatgtg tgcgatcgat ttcagccggg cggtcgcggg
     5881 acgcttcacc gcgagggcgt cggcgacgtc gaggccgtgc gcccaggttt ccatcagccg
     5941 tgcggtggcc atcgacgcgg cgctcatcgg cggaccgaac catggcagct tgcggccgtc
     6001 ggcgaccgcg agcagttcgt cgtgcagccg cccccgggtc tggcgccagt cggccaggag
     6061 ctgttcgggc ggaggtgccg cgagctcttc ggcgccagcg tcgacgaaac ccgtcggatc
     6121 cttggcggct tcggcgagca cgtctgcgaa gccggcctcg tcggtcaccg ccgtgagcgc
     6181 gacgcggtcc gtccacagca gatgcgcgat ctgatgggcg atcgtccacc ccggcgcagg
     6241 cgtcagttcg gcccaccggt cggcaggcag gtcggcgacg aggtcgtcga ggtcgtcgct
     6301 ttccgcacgg agatcagcca ccatcggctc cgctgtcgac atgccggtca cattagccgg
     6361 gtgattgcac gcgtcgaccg acgctggcgt gaacggccag ccctgcgagg tagaccactg
     6421 agccggcgaa caccagggcg ggctcgtggc cgtcggccgg aatcaccgtc gccgccaacg
     6481 tcatcgcgac gatgaacgcc atccagaaca gcgcgtcctg gacggtgaac acgtggccgc
     6541 gcagcgcgtc gtcgacatcg atctgcatcg cggtgtctgc gcacagcttg accacctgtc
     6601 cggccgcgcc cagcagaaaa ccgcacagca ccatcacggg aagctgtaag ccggagccgc
     6661 acagctgtac ggctgccgcg aaggccagcg cgccgttcgg cgcggcgtac cggccccagc
     6721 gacggacgat gctcggcgtc agtgcagtcg cgagaaacga gccaagaccc acggcggcga
     6781 ggaacatcac cgacgtcccg aatcccgcga cttccttagt gtcggtgtgc cgcaccatca
     6841 ccagcaccag cagcgtgttg atgcctaaca ccattcgatg ggcggccagc cccgacaacg
     6901 tggccgcgac ggtcggcacg gccaacaccg tgcgcgcgcc gtgcagccac ccggtggcta
     6961 ccgcatacat gaccgagccg tggatcgacc gcttgctctc gtgcggcccg aggatgtgcg
     7021 gtgggaaacg caccgacaac aacagcgcaa gggcgaccgg caccgtgacg atgaaaatga
     7081 ttgcggcggc cccggtgtcg tcagcgccga acagcgagcg tggcaccaac atgaagatcg
     7141 caccgaggaa cgccgcggcg gcgccggtgg cggtcgccac cgcgttcatc gtgaccacct
     7201 gctcgcgggc caccacgtcc ggcaaggccg ccgacagacc cgacgacacg aagcgggtga
     7261 aaccgttgac gatcaatgcg ccgaagagga tcagcgggtc gccggcgccc acggcgagca
     7321 gtgcggctat gccgagcacc aacaccagcc gcccgatgtt cgccccgatc agcacaagcc
     7381 gcctgtccca gcggtctagc agggcgcctg cgaacgggcc gagcaccgag tacggcagat
     7441 atagagcggc aaacgaaccg gcgatctccc acgggccggc ggcgcggtcg gtactgaaca
     7501 gaagcccgcc cgccagaccg gcctggaaca gcccgtcacc gaattggctg acggtccgca
     7561 gttcgagcaa ccgccaaaat tcaggtagcg cacgcaccga acgccatacg gcgatcggcg
     7621 cgcggatgtc ggccacggaa atcacgtcct ggttgagagc tgctgcagat cggcaacccg
     7681 acccacagta caaatatccc ggcgggccgc gcttgttcgc caccgcgacc cgttgccggt
     7741 gccattatgg tgaggtggcg cactcagaag acccggagga tttcctcgct cccgcggctc
     7801 accgggtgcg tgccggaaca ttgctgctgg cgaacaccga tctgctcgag ccgacctttc
     7861 ggcgcagcgt catctacatc gtcgagcaca acgacggcgg caccctcggc gtggtgctga
     7921 acaggccaag cgagaccgcg gtctacaacg tgttgccgca gtgggccaag ctcgccacca
     7981 agcccaagac gatgttcatc ggcggacctg tgaagcgtga cgccgcgctg tgtctgggga
     8041 cgctgcgggt cggtctcgag gcggacgggg tgccggggct acggcatgtg cagggccgga
     8101 tagtgatggt cgacctggac gccgatcccg atgcgatcgc gccaatggtc gagggggtac
     8161 gaatctttgc cggctactcg ggctggacga tcggtcagct cgagggtgag atcgagcgcg
     8221 atgactggat tgtgctgtcc gcgttgccat ctgatgtcct ggtcgaggcg cgggtggatc
     8281 tgtggtcgcg ggtgcttcgc cgccaaccgc tgccgatgtc cctgctggcc agtcacccca
     8341 tcgacatcag ccggaactaa gtcgcaggca ccgtccgcga cattcggctc gcaatcaccg
     8401 cgcagacgaa tagctggatc tggtgaaaga tgatcagcgg caacatgatc aggcccacgg
     8461 ctccggtgcc gaagaacacc agcgccatcg gcaggccgga cgccaggctc ttcttggagc
     8521 cgcagaacag cactactgcg gcgtcggcgc gattcagcga ggcgagtcgt gagatgccca
     8581 cagtgacgcc gagcatgagc gccagcagcg ccgtgcacac ccccgcgacc gccaccaccc
     8641 gccacgggct caccgtgctc cagacgtgtt ctgtgatgct catcgagaaa gcggtgtaga
     8701 cgacgagcag gatcgagccg cggtcgaatg ccttagtgac caacgcatgc cggtgcaccc
     8761 atggggcgag ccacggccgt agaagttggc ccgcgatgaa cggcagtaac agctgcacga
     8821 cgatgtccag gaccgccgaa ccgtcgatgc gcggcgcacc ggtggtgcgc atcagcaaaa
     8881 cgaccagcag cggggtgagc acgacgccga ggatgttcga cagcgacgcg ctcacgatcg
     8941 cggcgggcac gttgccgttc gcgatcgacg tgaacgcgat cgaggactgc acggtcgacg
     9001 gaaccagaca caggaacaac accccggtgt agagatcggg cgtcaacacc gacggcacca
     9061 ggccacgtgc ggccaggcca aggagcggga acgcgacgaa ggtcgcggcc agcaccaaca
     9121 gatgcagctg ccagtgccgc agcccgtgca aggcctcggt cggtgacagc cgcgcgccgt
     9181 agaggaagaa cagcagcgcg atggcgacct tggtggccac cgatagcacg tcgccgatcg
     9241 tgccgctgcc cggggaagag cgccgcgagc gccacgacag ccaacagcgc cagcaggaag
     9301 gtgtcgacgg ggagccgctt gagcacgcga gctcagttac aggacagcgt gcaggtcgcg
     9361 cacgctccgc cgcagccgcc cgcagctgga gcggtctggg cgcgggctcc ggcgctattc
     9421 gcggctgccg ccgcggccac cttggcgctc tgccaacacc cggccgcgac ggtgctgatc
     9481 gcgccgatca cgcagacgac tacaacgagc aagccggtct gcgggcgcga cgacaatgcc
     9541 acggcgccgc cagcagccag catcaccgcg gcggccaact gcgtcggcgc gaccccgcgc
     9601 aggacttgac ggacgaaatc gccagagcgc gggcgggtca gcagccagac cccaagtcct
     9661 gcggtcgcga cggcggcgca caagcagagc accgcggcga tcagcatgcg ccacacaata
     9721 cgaggctcgg acacggtccg ccggatgggc gtccccccgg gcgccaggct tcgcccgggg
     9781 ggaccgtcta tcccgtttgc cctagcggct gcggctgggc ccccagcgag ctgggggacg
     9841 ccggtaactg gggcaggccc ggcgccgggg gtagcggcgt cggggtcggt ggcccgggga
     9901 ggctcacctt gaatccgttg acgatcgcat cggtggaatc cgctgacgcg acaacctggt
     9961 cgacgctcgt cgtcaccgcc agcgacacta ggaatttgtc agggccggcc gtggcgatga
    10021 catggcgtcg cgacgtgttc agcgtcatcg agttctcgcg gtaggtgccc tcgataaacg
    10081 atgagggcat gccgccgaag tcggcgagcg acccgtcggt ggcccgccac gcaggcagca
    10141 actggctgtc gatgaagccg tggctgatgg cctccttcgg atcgaagtcg ccgatcagct
    10201 tgtagacctt gacctgggcg ttggtcgagt acagcccgtt gccgccgacc cggtcggcga
    10261 tcaccgcgaa cgcgtcgggc acattcggat cgggcacctg cgcccagccc ttaggcatcg
    10321 gcagcacgat gttgagcgcc ttgaagtcgc ggctgttctg cggctcgagc ttgacggcct
    10381 tcgccttgaa gaaatcgtcc agcgtccccg agctcgccgg ggtgatcgtc acaggcggcg
    10441 gggatggcgc cggtgtggcc ggcgtcacgc cgggctgccc gagggcttgc ggcgcggtgg
    10501 gcacggtgac cgtctgcgtg acggaagcag ggccgggcag tggcggttgc gggaacaccg
    10561 gctcggctga agcggtgccc ccagcgaggc cgacgacggc ggccacaccg gctgccgtgc
    10621 cacctgccag aatccgccag cggcgggcga tctcgttcat cgacggcggt ccttccgagg
    10681 cgagcgggat tcgtcacatc ccaggtcagg cgccgaccgt atcccgtgcg cagcgttagc
    10741 caccagggct ggaatcgacc tggaaccaac ccctgatcgt gtggtgacgc aaccgagacc
    10801 atcccgtggc cgacgaggcg gccatgtgcg ggcttatacg ctgttcacgt gacagagccc
    10861 gaggtcgaca ccccgcagca ccgctacacc gcggagctgg ccgggaagat cgagcacgcc
    10921 tggcagcaga tctgggcgga gtcggggacc ttcaacgtgc ccaacccggt cggctcgctg
    10981 gcaccgccag atgacgcccc ggtgcccgcc gacaagatgt tcgtgctgga catgttcccg
    11041 tatccgtcgg gtgagggatt gcatgttggg catccgctcg gctacatcgc caccgacgtc
    11101 tacgcgcgct attaccggat gacgggtcgc aatgtgttgc acgcgttggg cttcgactcg
    11161 ttcggcctgc ccgccgagca gtacgcgatc caaaccggta cgcatccgcg catccgcacc
    11221 gaggcgaaca tcgtcaactt ccgccggcag ctgggccggc tgggcttcgg gcatgacgcc
    11281 cgccgcagct tttcgaccac ggacgtcgac tactacaagt ggacgcagtg gatcttcctg
    11341 cagatctaca acgcgtggtt cgaccccgcc gcgaacaagg cgcggcccat caccgaactg
    11401 gtcgccgaat tcgaatccgg tgcaaggcgt ttggacgatg gtcggcagtg gtcggagttg
    11461 tcggctggtg atcgcgccga tgtgatcgac tcgtatcggt tggtgtatcg ggccgactcg
    11521 atggtcaact ggtgccccgg cctcggcacg gtgttggcca acgaggaagt gacatccgac
    11581 gggcgcagcg agcgcggaaa cttcccggtg ttccggaagc ggttgcggca gtggatgatg
    11641 cgcatcactg cctattcgga tcggctgctc gacgacctcg acctactgga ctggcccgaa
    11701 aaggtcaaga ccatgcagcg caactggatc ggccggtcca cgggcgcatc tgtgctgttc
    11761 ggtacggccg ccggggacat cgaggtattc accactcggc cggacacgct ttttggtgcg
    11821 acgtacatgg tgctggcgcc ggagcacgaa ctggtggaca agctgacggc gtccgagtgg
    11881 ccggccggcg tcgacgaacg gtggacattc ggctcggcaa cgcccgccga ggccgtcgcc
    11941 gcctatcgcg ccaccatcgc cgccaagtcg gatctggaac ggcaggagaa caaggacaag
    12001 accggcgtct ttctgggtgg ctacgccacc aatcccgtca atggtcaaca gattccggtg
    12061 ttcattgccg actacgtgct gctgggctac ggcaccggcg cgatcatggc ggtgcccggt
    12121 ggcgaccagc gggactggga cttcgcgcat gcattcgggc tgccgatcgt ggaaacggtt
    12181 gccggcggcg atatttcgca gggtgcctac ggcggtgacg gcgaggtggt caactccgat
    12241 tacctgaacg gtctgtctgt cgctgcggcg aaggaggcca tcacccggcg gctggaggcc
    12301 gacggccgtg ggcgggcccg catcgagtac aagctgcgcg actggctgtt cgccaggcag
    12361 cggtactggg gcgagccgtt ccccatcgtg tatgacgccg acgggcgtgc gtacccgctg
    12421 cccgagtcgg cgctgccggt cgagctgccc gacatcccgg actacgcgcc ggttcagttc
    12481 gatccggacg atgcggacag cgagccgtcg ccgccgctgg gcaaggcgac cgactgggtg
    12541 cacgtggagc tggacctcgg cgacggtctc aagccgtaca gccgcgatac caacgtgatg
    12601 ccgcagtggg cggccagctc gtggtacgag ctgcggtaca ccgacccgca caattcagaa
    12661 gagttgtgcg ccaaggagaa cgaggcgtat tggatgggtc cgcggccggc cgagcatggc
    12721 gccaaggacc cgggtggtgt cgatctgtat gtcggcggcg tcgagcacgc ggtgctgcat
    12781 ctgctgtacg cgcggttctg gcataaggtg ctctacgacc tcggccacgt cagctcgaag
    12841 gagccgtatc ggcggctggt gaaccagggc tacatccagg cgttcgcata caccgattcc
    12901 cgcggcgcgt atgtgccagc cgccgaggtc accgaacgcg acggcacgtt ctactggacg
    12961 ggcccggacg gcgagatcga ggtcaaccag gagttcggca agatcggcaa gagcctgaag
    13021 aactcggtgt cacccgacga gatctgcgac aactacggcg cggacacgct gcgggtctac
    13081 gagatgtcga tgggtccgct ggaggcgtcg cggccgtggg ccacgaagga cgtggtcggc
    13141 gcgtacaggt ttctgcagcg ggtgtggcgg ttggtggtcg acgagcagac cggtatcact
    13201 catcccgccg agcacgaggc cttggacgac gagacgctgc ggctgctgca ccgcacgatc
    13261 gcgggagtcg ccgacgacta cgcgtcgctg cgcaacaaca ccgcggccgc caagctcatc
    13321 gagtacacca accacctgac caagcaaggg gtgcgggcac gggcggcact ggagccgctg
    13381 gtgctgatgg tcgcgccgct ggccccgcat ctggcggagg agctgtggca gcggctcggc
    13441 cacgacaagt cgctggcgca cggcccattc ccggtcgccg atccgcagta cctcgtcgag
    13501 gacaccgtcg agtatccggt gcaggtcaac ggcaaggtcc gcggccacgt gacggtgccc
    13561 gccgacgccg atgccgacac gatggaggcc gcggcgctgg ccgatgagaa ggtgcaggcc
    13621 ttcctcaacg gcgccgcgcc gaagaaggtc atcgtggtcg ccggccgctt ggtcaacatc
    13681 gtcgtctagt agcggcttgt ggagcgggac ccgcgctctc agcgcggctt cggctccaca
    13741 aatcacgccc agccgagctg gcgcatcgtg atcccgtcat tccagatctt tgtcatgtgg
    13801 cggatcttgt cgccgtcaaa gtccatcacg tagacgtaat cggcttcggc ggacttaccg
    13861 gtcggcggca ccggcccgcc gtcgcccgtg tgtgtcccgc ggaagacgcc gaaggcggcc
    13921 acgtttttcc gctcctcgtc gacggcgaac gaccgcactt cggcattgcc gtcgggcagg
    13981 atcgtcatca ggcccttcat ccactcggag tacgcctcga gagtggtcac atcagccagc
    14041 gcgtcggtct gcgccgcgaa ggtcgcgccg gggtgacagt atggctcgca ccccgaccaa
    14101 ccctttccgg tttcacacgc ctcgaaaaac cgttcggcgg tgtcttttat cgagctcata
    14161 accgattgtg cgcacttggt tgtgttcacc gcggcgaact gcgcagaaat catcgggggc
    14221 ggacgatcac ctcgttgatc tgggcatcgc gtggagcgtt gatcgtgtcg gcgacgactt
    14281 tcgcgacggt ctcggggctc aggaattgcg acgggtcgta atccttgcct tcgtaagcga
    14341 tcaggccttc ctgcatttcc gtcgcgatcc ggcccgagtg gatcgacgtc acccgtagcg
    14401 acggctcgtc gtcgcgcagc gagtccgcga acccgcggag cgcgaacttg ctagccgtat
    14461 acgacgccag ccccggcgac gcgttgatgc ccgcaccgga gttgatgaac acgacgtgac
    14521 cgccagcggc gcgcaacgct ggcagcagcg cgagcgtgag ggcaaccgcc ccaatgacgt
    14581 tgacctccat ggatttacgc cactcgtcaa cagaagactc tgcgacgcgg ccggggtagg
    14641 cgacacctgc gttgtggatc agcacgtcga gctcgacgat cggctcgacg accgcccgta
    14701 tcgcgtcggt gtccgccagg tcaacaggcc acgtcgtcgc accgaaccgc gcagccacct
    14761 cgtccagccg cgatgacggc cggcccgcca ggaacagcgt gtgcgtcggc gcaagcacct
    14821 cggcgagcgc cgaaccgagc ccgcgaccag ccccggtgat catcgcggtc ggcatgtcgc
    14881 aacactaccg atctgcaagt cccaggctct cgtcgcatta ttgaaccgat gccacccgac
    14941 catagcttcg ctccgaccca gctggcggcc cgcgccgcat acctgctacg cggcaacgat
    15001 ctgggcgcga tgacgaccgc cgcgccgttg ctgtacccgc atatgtggag ctgggacgcc
    15061 gcattcgtgt ccatcgggct cgcgccgctg agcgtcgagc gcgccgtcgt cgaactcgac
    15121 acactgctga gcgcgcagtg gaccaacggg atgatcccgc acatcgtgtt cgccaacggc
    15181 gtcgacggct actttccagg acccgcccgc tgggctacgt cggcgctcgc ggccaacgcg
    15241 ccacgaaccc gccacacatc gggcatcacg cagcccccgg tgcatgccat cgcggtgcaa
    15301 cgaatcctcg accacgcgcg cacccgcggc cgatccaccc gcgccgtcgc cgaggcattc
    15361 ctcgatcgcc gctgggccga cctcgtccgc tggcaccgct ggctggccga ggcccgcgac
    15421 ccgaaatccc gcggccgcgt cacgctgtac cacggctggg aatccggcat ggacaactcg
    15481 ccgcgctggg atagcgccta cgcgaacgtc gttccgggca acgtgccgga gtatcagcgt
    15541 gaggacaaca cgatcgtcac cgacgccagc cagcggccaa gcgacctcga gtacgaccgt
    15601 tacctgtggc tgctggagga aatgaagtcg gcgcgctacg acgacgagtt gctgcccaag
    15661 gtgatgagct tcgccgtcga ggatgtgttc gtgtcggcca tcctgtcggt ggcctgcaca
    15721 gtcctcgcag agatcggcga agactacaaa cggccgcacg ctgacgtcaa ggacctgtac
    15781 gcgtgggccg aacgatttcg taggggcgtc gtcgagacca ccgacgaacg caccggcgcc
    15841 gcaaaggatt tcgacctgcg cgccgacagg tgggtggcta ccgagacggt ggcgcagttc
    15901 gcgccgctgc tgtgcggcgg gctttcgcac gataaggagc gcaccctgct gcggctgctg
    15961 gaggggccac ggttctgcgg gcatccggat ctgaggtaca cgctgatccc gtcgacgtca
    16021 ccggtgtcgc gggacttccg ccagcgcgag tactggcgcg gaccggtgtg gccggtgatg
    16081 acatggctgt tctcgtggtg ctttgcgcgc cgcggctggg cagagcgcgc gctgatgctc
    16141 aaacgggaag ggctgcggca agccagcgac ggcaccttcg cggagtatta cgagccgttc
    16201 accggagaac cgcttggcag catgcagcag tcgtggaccg ccgcagcggt gctggactgg
    16261 ctgggctgac gcctattcgg ccagccgctc caacggaacc aacgccttgg tcagcgtctc
    16321 caggtcctcg gggctgagct tcgccatcaa cgtcgccagg tgtgcccggc gcgcggccag
    16381 cgcttcacga tgctgagata ggcccttcgg cgtgatgtcg acgagcacgg cccgcagatc
    16441 cgacggatcg cgcgaacgct tcaccagccc aagtttctcc agccgccgga tcgccaccgt
    16501 cgtcgtcggt gtgcgcaccc gttcgtgggc ggccagctcc gtcatccgga tcggcccctg
    16561 atcgagcaac gtcagcagaa tcgacaactg agccagcgtc agatcgccgg catccgtgcg
    16621 attcgtgtcc ccccggcgaa gcactgaaaa caccttggag agcactcgct gcagctcacc
    16681 ggcaagttcg ccgacctgcg attcggtctc caccataatt cgccagtcta acctgtcgga
    16741 ggctgccaca agcgaatgcg cgtcaattag cctcacaaaa cttgcgacag gaatcggcgc
    16801 agccgatccg tctcggcggc ctcgaaaatt cgctcggcgg accggattcg accacctggc
    16861 cgtgatccat gaacaccacc gcgtccgacg tcgagcgggc gaatcccatc tcgtgcgtga
    16921 ccaccacgac accgatgcgc tggccttcgg ccagcttgtc gaacccggtg atgggggagc
    16981 cagaaggcac caccagagag aagtagccga agtcgtagcc gttggtgaag ccgacggtct
    17041 tgcgtcgcgc atcggtggtg gtgatcgacg acgaaccgac gtcgaaacgc cgcgacgcca
    17101 cctgcgccag cagccccgag aactcggtgc cgacgaagtt caccttcagt cccagcttgt
    17161 cggcgatcgc gcgcagcagt tcgttgtcga agcccgtgaa ctggccggcc gagttgatgc
    17221 agatgctcgg cggcgcgtcc gacagcgtgc cgacggtcag cgtgcccggc acactcagcc
    17281 ccagcgcatt gacgttgacg gcggtcaacg gttcgaccgt cgcggtcgtg tacttgtcgg
    17341 cgccggggcc cgtcgccgct ttcccgaggt tggtcggcag cgcgctcgca ctctggatcc
    17401 ccggcggggc gcactggtcg acgttggcac cggctggcgc tgccagaccc atgctgcaga
    17461 ccagcatcgc aatcagggcc acgacggttg tcttgacggc ggtcaccaaa gcaaggtagc
    17521 cgccaagcgc tcgacggaat cgccttgcgc tcacctagaa cgcaatggac gccccggacg
    17581 ggcggaacac ccctcgccgc accagctcag taagcatgat cctggccatt aattctgcgc
    17641 gctcggcaca cgccgcgcgg gcaccgtcgg gatcgcgacg ttggatggcg gcggtctcgg
    17701 cctcgtagaa cggcaatatc tcgtcgtggt tgttgaggta ggcgagccag aacgtgcggg
    17761 ggatgaaggt ctgcgatgca cggatcgcgg cctgcaagcg tgggccggcg aattcgtcgt
    17821 tgatcacgcg gcggtacttc cacgccgtct cctgaaacgc gcgcgactct ttgctggccc
    17881 gcatgacatc gagaagcgcg ccgagcgtgt cgaggattcg gggaagacca tcgacggcgg
    17941 cgcgcgccga tgcgatgccg ctcagcaatc catacagctc gtgatgttca cgcacgacgg
    18001 actcgtcgaa tcgttcgacg tacgcgccgc ggtgatactg cgtcgacagc acgccgtcat
    18061 gttcgagctg gaccaccgcc tcctggatcg ggacgcggct gagcccgagt tcctgcgcga
    18121 tctcgttgcg atcaatccgg tcccccgacc gcagtttgcc agtcagcacc aggttgacga
    18181 catgcgagac cacgaggtct ttttctttga ccccgtactt cttgggcatg gcgcggcaag
    18241 catctcacgt agccgtcgcg ccgggagccg ttccgccagt tcagtttcca ttctgttggc
    18301 gacggtcgcg ccaccggcac aacgcctcgg cctgtgtcag gtcgtagtcg ggtccgttga
    18361 cgccgacggt cagcatcgtg acgccgagtt ccacgaacgc gccggcctct tccagcagcg
    18421 catcgaggtt ggcgcccgcc acaccggcag agcgctcgat ggtcgccggg tcgcgtccga
    18481 cgtcggcgca gtgacgtgcc aggatctccg ccttcgccgg gtacgtctcc agcgaggtga
    18541 acccgtgcca gatatctccg tgctcggcga caaggcgcaa cgtcttcttc tcgccttggc
    18601 cgccgatcag gacggggatg tcgcgggtcg gcgcggggtt gagcttggcc agccgcgacg
    18661 tgatccgcgg gaacgcgtcg gccagatcgt cgagccggct gcccgctgtg ccgaactcgt
    18721 agccgtaatc gtcgtagtcc ttctgcttcc accccgaccc gatcccgagg atcagcctgc
    18781 cctcggagat gtggtcgacg gtgcgcgcca tatcggcaag cagctccggg ttgcgatacg
    18841 agttgcacgt caccagcgcg ccgatctcga ggcgcgacgt ctgctcggcc caggcgccga
    18901 gcatcgtcca gcattcgtag tgcggaccat cgggatcgcc gtagagcggg aagaagtgat
    18961 cccagttgaa cgcgacgtcg acaccgatgt cctcacagcg acggacggcg tcgcggatgt
    19021 ggctgtattg cggggagtgt tgcggctgca gttgcacgcc gatgcgaatg gggaagctca
    19081 ctgtcgtcag gctagacgcc gatttgaccg ttggccttcc acaccgcgac gacggacggg
    19141 cgcgcggtgc cgtttccgcc ctccggccag tgcgacagcg gcttatcgat ggtgtagtcg
    19201 tcgccctcgc cgggatgctg cacgcagact gtgacgagat cgtcggtcac cacgggtccg
    19261 caggtttccc cacccttcgg cacggtgagg aactgtttgg tctcaccgcg attcggccct
    19321 tccagcgcga cggcgaacaa gccgtcgttt gacttcagcg cgttgccgtc ggtggagatc
    19381 cacagattgc cgtggctgtc gaaggccacg ttgtcggggc aggagatcgg gctgaccttc
    19441 gtcttgtcga atccggcgaa gtaggtgtcg gctgccgcgg gatcaccgca gacgagaagg
    19501 agatcccagg tgaagtcggt gcccgcgtgg ttgtcggtga tctcgaggat ctgcccgttc
    19561 ttgttctcgt tgcgcgggtt ggcggcgtcg acgggagcct tgccctcggc gccgcgcttg
    19621 tcgttgttgg tcagcgcgac gtagaccctg ccggtcttgg ggttggcctc gaagtcttcg
    19681 ggccgatcca tcttggtggc gccgaccttg tcggcggcga gccgggtgaa caccgccgcc
    19741 tcctgtgcgg tcaccccgtc gacgagcgac tcgccctgac cgttggcgcc ggagcgcagc
    19801 agtgggatcc acgtgccgct gccgctgaac gccccctcct tcggcagctt gccggagccg
    19861 tcgatctcgt cggccgggat gtcgctggac agctttgcga catacagcgt gccctcgtcg
    19921 aggatcgtca tgttgtgggc catcgcgacc ggatcggtac cggcctggat cttcttcgcc
    19981 gagacgaact tgtacatgta gtcgaagcgc tcgtcgtcgc cggcgtaggc caccacggtt
    20041 ccgtcgctgg tcacgtagac gttggcggct tcgtgcttga accgccccag cgcgctgtgc
    20101 ttgacgggct tggagttggg atcccacgga ttgatctcaa cgacgtatcc gaagcggttc
    20161 acctcgttgg gggtcttggc gacgtcgaat cgcggatcga aggactccca cttgagctcg
    20221 gtcggctcaa gcgtgacgcc gtagcggtcc tgccggtcgg cgtcgacggg accgggctcc
    20281 ggcgcaccct ccggtgcacc gaagtattcg tgaaagttct cttcaccgga aagcacagtg
    20341 ccccaagggg ttacgccgcc ggagcaattg gcgaaggtgc ccgccacggt gcggccggcc
    20401 ggatcggccg gcgtcttgac gaagtcggtg cccgcggcgg gaccagtcaa cgtgaacggt
    20461 gtgtcggcgg tgatgcgccg gttgtacggg ccgattaccg gcttcagtcc ctccggcgtg
    20521 cgctcgacct cgacgacgcc catcccgatc gccgcgatct cgatgtcgaa ctgctcgcgg
    20581 gtcggcttcg cgtcgtcgta gccggggaac atgaactgcg ggcttacgta ttcgtggttg
    20641 gtcaccagca ggaagcggtt cttttgaccg ggcacgggca gcagtccggc gaagtcgttg
    20701 ttgaacccga actggccgcg ctgcgcggcg gcggtctgcg ccctcacgtc aaacttcggt
    20761 gcgttcggca ggatcgggtc gccccagctg atgaccactg cctgctggta gccctccggg
    20821 atcacgaccg cgtcctcttt gttgggcgcg acggcggtga agttcatgcc aggagggggc
    20881 tcggcgggcg ccggcgacga ggtgggcgtc gcggcgggct gctcaccctg cccgcatgcg
    20941 gccagcgccg aacccgcgcc gacggcgagc actgcgatgc cgccggcctg caacattgac
    21001 cgccgcgaga cggccttcac gatgtcgccg aagtactcgt tgtcgctggt gttgggtgcg
    21061 ggtttggagc aggcgtcacc gcacttgtag cggcacgtca catgctggcg cgacgaccgg
    21121 ccgtcgtggg agacaagaag attcaacggc acaagcgcca tgggtgcggg ttccttccga
    21181 caaccggccg gggctggcgg ccggactcgc gaaacctacg acagtttggc aatcagctgg
    21241 caagcaatcg gtgaacaact ggggacgcta agcgcccagc accccccgca ggatctcgac
    21301 gagcgcggcc ggctggtcgc cctgaacgga atgtccggag tcggcgacga cgtgtgtgcg
    21361 ctggaagccc ggcgcggcct ttgcgaacgc gtcggcgtcc tcgtcgttga cgaagtacga
    21421 gttggcgccg cgtacgagcg tggtcggcat cgtgatggcc ggcacgtcgt cccacagccc
    21481 gtcgaagccg tcgcccttgc ggaacgagtc gtagcgccac gtccaggtac cgtcgtcgag
    21541 ctgcttggaa ttgtgaaaga cgccgcggcg caaagatttc cggtcacggt gtggcgccgc
    21601 agccaccgtc acgtccagca tggccgcaaa cgtgggaaat gttcggtcgc cctgcaccag
    21661 tgccacggtg cccatctggg ccttcgtcat ctgttcgtgg cgttcgggtg ccgacggcgt
    21721 gacgtcgacg agtacaagct ccggcacaag cgacggttcg gacgctgcga tgcgcagcgc
    21781 ggtgagcccg cccaacgaca tgccgactac cagccgggga ttcggtgccc actcgcgcag
    21841 caggggacgc agcgtctccg cattgagcca cggaccgtag tcgccgtcct cacgccacgc
    21901 cgatcggcca tgccccggaa ggtcgatcgc caacgcgggc agaccgagcc cgacgatgac
    21961 ggtgtcccag gtatgagcgt tctgcccgcc accgtgtagg aacaccacct gcggcggctc
    22021 gtcaccccac ttgagcgcgc tgatggaccc cgactcgatg cgctgcaccg acggcagcgg
    22081 cccgccgacc ccgatttgtt cggcgttctc gtgaagcagc ccgaactcgt cgaggccggc
    22141 caggtcgtcg tcggagactt cggcgttcgt cacgccgttg aggctagcgc tgttacgcct
    22201 tcggccggcc ttcgctgtag tgcgccggcc gcttggccga cggcgcggtc gctgtcgtgc
    22261 gctgcggctg tccggagggc tcctcagccg cccacttgta gtcggactcc ggtgcgcgtt
    22321 cggaggccac cgtgcccacc ggtgcggcgt ccgcgctgga gcgccgctcc cgaatgctgc
    22381 ggccttcgcg ctcggccgtg gtcagatagt cctcccagcg cgcctgcatc ggcttgatca
    22441 ggccaccgcc gacgccgacc acgaggatgc cgccgattgt cgccaagacc gcaaccagga
    22501 ccggcagcgt cacggtcagc gcgatgccca cctgattgag cgccgcgatg atgcccaatc
    22561 cgaggatgaa gacgctggca agagttccga gcgcccggcc gtacgagaat ccgctcagcg
    22621 cgtttgacac gatgtcgcga accgcagccg cgatcgcggc ggccaccacg acgatgatca
    22681 gtgcgacgaa cagtcggggc aggaaagcga tcacagcggc gatcagttcg ctcaccggat
    22741 tcggtccgaa gacgccaaag gcgaaattca gaacgatcag caataacccg tagtagacga
    22801 tcttgctgac gatggtcgag gcgtcatagt tgcttcgctc catggcccgc cgcataccgc
    22861 cgcgctccac cgcgcgatcg aaaccgacgc gttcgaggat tttgtcgacc gccttggcga
    22921 tcagtttcgc cacaatccag ccgacaatca gaatgatgag gaagaggaca agctttggag
    22981 cgatggcgag caccgacccc cacatgtctc tgagttggtc ttgcatgacg tgctccttgg
    23041 atcgaggctc gaccggccac agaaattatt gacaccagcg tcataccccc cggtcaactg
    23101 cgtaaacctc gaaccgcccc gacaacagca acatgcaaaa cggggacccc agatggggtc
    23161 cccgcattgt gccggcgttg ccgtatcagc ccttgatgta gtcctcgagc tgcgcgcggg
    23221 cgatgtcgtc gggcagctgc ttcggcgggc tcttcatcag gtatgccgac gcggccacca
    23281 ccgggccgcc gatgccgcgg tccttggcga tcttcgccgc gcgcaccgcg tcgatgatga
    23341 cgcccgcaga gttcggcgag tcccacacct cgagcttgta ctccagattc agcggcacgt
    23401 cgccgaacgc gcggccctcc agccgcacgt aggcccactt gcggtcgtcg agccagccga
    23461 cgtgatcgga cggaccgatg tggacgtcct tggtcttgaa ctcgcgctgc aggttggagg
    23521 tgacggcctg cgtcttggag atcttcttgg actccagccg ctcgcgctcg a
//
LOCUS       DASRJA010000762         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2654438,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000762 DASRJA010000000
VERSION     DASRJA010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2654438"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VFC01_34525"
     CDS             complement(<1..686)
                     /locus_tag="VFC01_34525"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HZN84771.1"
                     /translation="MSLTWRRALAWRMRRQLLDPVGTAPVAGVVRRLCAVPAQFDTAA
                     ELAIGARREGFRPGEVARALAAGRIIKTFAFRGATHLMTPEDGGTYLALRAASRMWEL
                     PSWQSFYGLTPADWPLLREVVREALADGPLTRDELGAAVTARPRFRHLGFAFGDGSGT
                     LLKPLAWQGDMSFGPRRDGRATFQRLDSNPRWAGVPDLDKAGAQAVEAYFKAYGPATP
                     DHLHYWLGNGL"
     gene            complement(1004..3331)
                     /locus_tag="VFC01_34530"
     CDS             complement(1004..3331)
                     /locus_tag="VFC01_34530"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZN84772.1"
                     /translation="MPAQPRRFRRATDGVLLAISVVVLIVCAAGADEPPSDAETALGS
                     LLSNLPGILQPVWQISFDLLQVWAVVLLVLALARRRWRLARDLVAVVAAVAVAGAAIG
                     RLATGEWPQVLNGLGAANDPIDFPSLALAVGAGAVAVAAPYLARPLRYLGRWLVVLGW
                     LAAVAVEVLSPGGALGALALGWAAAAFVHLLAGSPGGQPSLSEVREELRAIGIEAEPH
                     SIRTAAGVAHVTAKTPDGIELDVRVYGRDAWDSQLVVKLWRLLWYRSGGPDVALTRRQ
                     QIEHVAFLTLLAERRGVAVAPVIAAVVDERGDALLVVERVGPGLGDAAPEVDDAVLSS
                     CWRTLVDLHAGGIRHGAIAPEHLQLVDAAVHLSDFDLAVVGSDAESQRLDQAQLLATT
                     AVVADIDRATAVAREALGDDGLAAVTPYVQPAALTASLRRQAAAADIDVDALRKNALG
                     AIGGQDETLQPLRRFSLGRLVMGVLLLIVAVTLVSALQNIGVDAIVDAIQAASVPVLI
                     VAFLIGQTPRLANAVSLAIAAPLRIPLPRLAALQYAITFINLAVPSTAARVAVGIRFF
                     QRCGLDRTSAITVGALDSFAGFVAQIGLILTILLFGLGTLDLNLDETLSYDAVESLLI
                     FLGIVLVLVIAAAMLIPSLRRLVSAAVHLVRTKIWPMLASPRRLIGVLAANVVVELLF
                     SLCSYLVLASFGQDVAFADVILVNEAVAIFAGIMPVPGGMGVTEAALTAGYTAMGVDS
                     ATAVGAAIMYRIITFYLPPVIGYPALKSLRRDSYL"
BASE COUNT          643 a         1256 c         1175 g          508 t
ORIGIN      
        1 aggccattgc cgagccagta gtgcaggtgg tcgggggtag ccggcccgta cgccttgaag
       61 tacgcctcga ccgcctgcgc ccccgccttg tcgaggtcag gcaccccggc ccaacgcgga
      121 ttgctgtcca ggcgctggaa ggtcgcacgc ccgtcccgtc gtgggccgaa gctcatgtca
      181 ccctgccacg cgagcggctt cagcaatgtg ccggacccgt cgccgaacgc gaagcccaga
      241 tgccggaacc tgggccgggc ggtaaccgcc gcgccgagct cgtctcgggt cagcggcccg
      301 tcggcaagcg cctcgcggac aacctcccga agcagtggcc aatccgctgg ggtcagaccg
      361 tagaagcttt gccaactggg gagctcccac atccgactgg cggcccgcaa agcgaggtag
      421 gtgccaccgt cctcgggcgt catcaaatgg gtagcgccac gaaacgcgaa cgtcttgatg
      481 atccggcccg cagcaagcgc gcgagcaacc tcgcctggtc ggaacccttc gcgcctggca
      541 ccgatggcga gctcggccgc ggtgtcgaat tgggccggca cggcgcacaa acggcgcacg
      601 acgcccgcga ccggtgcggt gccgaccgga tcgagcaact gccgccgcat acgccatgcg
      661 agggcccgac gccaggtgag tgacaccgtc gcacgctatc ccaccgcgac cgctctgccc
      721 gcgcctcgcg tcggtgatgt cgaggatcga catgatcgtg ctaaggacca cgacgccggc
      781 gatcttgaga acagccgcgt cgatgtgatc ggggtactcg gtctgggtgg ccggggccgc
      841 cattcaatgc tcctcccgcc ttgcggtgcg ttcgtcaccg ccaaggcggc gcgcttcgca
      901 gcctggccac gtcaccgaca agcccatcgt gactgcagct tccgctcacc ccacgcaaca
      961 cccgaccggc agccgtcttc ccgtcgcgcg cagcaaggct cggctagagg tagctgtcgc
     1021 ggcgtagcga cttcagggct gggtagccga ttactggcgg caggtagaac gtgatgatgc
     1081 ggtacatgat cgcggcacct acggcggtcg cgctgtcgac tcccatcgcg gtgtatccgg
     1141 cggtgagggc cgcctcggtg acacccatgc cgccgggcac cggcatgatg ccggcgaaaa
     1201 tggccacggc ttcgttgacg agtatcacgt cggcgaaggc cacgtcttgc ccgaaggaag
     1261 ccagcaccag atagctgcac agtgagaaca gcagctcgac cacgacgttg gcggccagca
     1321 caccgattag ccggcgtggt gaggcgagca tcggccagat cttggtccgc acgaggtgaa
     1381 ctgcggccga gacaaggcgg cgcaggctgg ggatcaacat cgcggccgcg attaccagaa
     1441 cgagcactat cccgaggaag atgagcagac tctccaccgc gtcataggac agcgtttcgt
     1501 cgaggttgag gtcgagggtg ccgagtccga acaacagaat cgtgaggatc agcccgatct
     1561 gggccacgaa cccggcgaag ctgtcgagcg caccaacggt gatcgccgag gttcgatcaa
     1621 gaccgcagcg ctggaaaaac ctgatgccca cggccacccg ggctgcggtc gacggtacgg
     1681 cgaggttgat gaatgtgatc gcatactgca acgcggccag ccgcggcagg gggatccgca
     1741 acggtgccgc gatcgccagc gagaccgcgt tcgccagcct tggtgtctgg ccgatcagga
     1801 acgcgacgat cagtactggc acgctggcgg cctgtatggc gtcgacgatc gcgtcaacgc
     1861 cgatgttctg cagcgcactc accagggtga cggcgacgat taggagtagg acccccatca
     1921 ccagccggcc tagcgagaac cggcgtagtg gctgcagcgt ctcgtcttgg ccgccgatcg
     1981 cgccgagcgc gttcttgcgc aacgcgtcga cgtcaatgtc ggccgcggca gcctgcctgc
     2041 gcaggctggc cgtcagcgca gccggctgca cgtatggggt gaccgctgcc aggccgtcat
     2101 cgcccagcgc ctcccgggct accgccgtgg cccgatcgat gtctgcgacc acggccgttg
     2161 tcgcgagcag ctgcgcctgg tcgagtcgct gagactcagc gtcggagccg accaccgcca
     2221 ggtcgaagtc gcttagatgc acggcggcat ccaccagctg taagtgttca ggggcgatgg
     2281 ctccgtgccg gatacctccg gcgtggagat cgacaagggt gcgccagcac gacgacagca
     2341 ccgcgtcatc cacttcgggc gctgcgtcgc cgaggccggg accgacccgt tccacgacga
     2401 gcaatgcgtc tccgcgctcg tcaacgaccg cggcgatgac cggcgccacc gcgaccccgc
     2461 ggcgctccgc gagaagtgtc aggaacgcca cgtgctcgat ctgctgacgc ctggtgagtg
     2521 ccacgtcggg cccgccggac cgataccaca gcaggcgcca tagtttgacg acgagctggc
     2581 tgtcccatgc gtcccgcccg tatacccgaa cgtcgagctc gatgccgtcc ggtgttttgg
     2641 ccgtcacgtg tgcaaccccg gccgcggtcc ggatgctatg cggttcggcc tcgatgccga
     2701 tcgcccgtag ttcttcgcgc acctcgctca gtgatggctg cccgccgggt gagccggcga
     2761 gcaagtggac gaatgccgcg gccgcccatc caagtgcgag cgcgccgagc gcaccccccg
     2821 gactcagcac ctcgaccgcc accgcggcga gccaaccgag aacgaccaac catcggccga
     2881 gatagcgcag cggtcgcgcc aggtacggcg ccgctactgc gaccgccccc gccccaacgg
     2941 caagcgccaa ggatgggaag tcgatcggat cgttcgccgc tccaagcccg ttgagtacct
     3001 gcggccattc cccggtggcc agccgcccga tcgccgcccc ggccaccgcg accgccgcga
     3061 cgacagccac caagtcccgc gccagcctcc atcgccggcg ggcgagcgcc aggacgagca
     3121 gcaccaccgc ccacacctgg agaagatcga aggagatctg ccagaccggc tgcaggattc
     3181 cgggcaggtt cgacagcagc gaaccgagcg ccgtctccgc atcgcttggc ggctcgtcgg
     3241 cacctgcggc gcacacgatg agcaccacga cggagatcgc cagcagcaca ccgtcggtgg
     3301 ccctgcgaaa tcggcgcggt tgggccggca cggcaaacgc ccgagtcccc agacgaccca
     3361 gaccgggctc tgcagcgcta acgtcgcccg acagggtgcc ttcccgccgc ggaccactgg
     3421 ccgctggcgg tgcaacgtaa aacctgcccc gcctgccagc gccggacggc aagtggcctc
     3481 cccaaaagca gtgtgcccag tagcgcgacg ttggtcttgg tgatcccgaa aagagtatgc
     3541 agcgagatcc acttttccgc cgatggcaaa ccggggagat ca
//
LOCUS       DASRJA010000763         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2895037,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000763 DASRJA010000000
VERSION     DASRJA010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2895037"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1562)
                     /locus_tag="VFC01_34535"
     CDS             complement(<1..1562)
                     /locus_tag="VFC01_34535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN84773.1"
                     /translation="MASADAAEYKQVTVLFADVVRSMDMAAALDIERLREIMTELVNR
                     CAAVVLRFGGTVDKFTGDGIMALFGAPVALEDHAVRACLAALDVQKEAVRLAADVERR
                     DGISFQLRVGLNSGQVITGEIGSGSLGYTAVGEQVGMAQRMESVAAPTGVMLSESTAR
                     LVEHAAVLGEPEWVHIKGADAPVPARRLLGMTSGHVRQRQEASLVGRHSEMAALEALV
                     DRALGGRGGVVNVVGEPGIGKSRAAREAAALAASRGFEVVWAFCESHASDIPFHAVRR
                     LLRALSGVEDLDGPAARIRVRERVPDADPEDLLLLDDLLGIADPDTPTPAIDPDARRR
                     RLTALINTAAEARAAPALFIIEDVHWVDAVSESMLADFLTVIPQTKLMVLITSRPEYE
                     GALTRAPEAQTIALGALDDSDTTALLGELLGSDPSVADLAAVIAERAAGNPFFAEEMV
                     RELVQRAVLEGERGGYLCRVDVADVAVPATVQAAIEARIDRLSTAAKRTVNAASVIGA
                     RFEAELLAALGI"
     gene            1802..>2466
                     /locus_tag="VFC01_34540"
     CDS             1802..>2466
                     /locus_tag="VFC01_34540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZN84774.1"
                     /translation="MSNTGPLAGVKVIELGGIGPGPHAAMVLADLGADVVRVRRPGGL
                     QIPAENLDLLHRGKRIVDLDVKNQRDKLLELVAKADVLLDGFRPGTCERLGIGPDECA
                     AVNPRLIYARITGWGQDGPMAKAAGHDINYLSQTGALSAIGYRDRPPVAPLNLVADFG
                     GGSMFVLLGIVAALYERERSGKGQVIDAAMVDGVSILAQMAWTMKSTGSLKDERESFM
                     LDGG"
BASE COUNT          438 a          892 c          770 g          366 t
ORIGIN      
        1 tcgatgccca gcgcggccag cagctcggcc tcaaagcggg ccccgatcac cgacgccgca
       61 ttcaccgtcc gcttggccgc ggtgcttagc cggtcgatgc gtgcctcgat ggccgcctgc
      121 accgtggcgg gcaccgcgac atcggcgaca tccacccgac acagatagcc gccgcgctca
      181 ccctccagca ccgcgcgctg cactaactcg cgcaccatct cttcggcgaa aaacgggttc
      241 ccggcggccc gctcggcgat gaccgcggcc aggtcagcga ccgagggatc cgaacccagc
      301 agctcgccca gcagtgctgt ggtgtccgaa tcatccagtg cgccaagcgc tatcgtctgc
      361 gcttccggcg cccgcgtcag cgctccctca tattcggggc gcgaggtgat cagcaccatc
      421 aacttggttt gcgggatcac ggtgaggaag tcggccagca tcgactcgct gaccgcatcg
      481 acccagtgca catcctcgat gatgaaaagc gccggtgcag cacgggcctc cgccgcggtg
      541 ttgatcagcg cggtcaaccg gcgccgccgc gcatccgggt cgatggcggg cgtcggcgta
      601 tcggggtcgg cgatgcccag cagatcgtcg agcagcagca gatcctccgg atcggcatcc
      661 ggcacgcgtt cgcggacccg gatccgcgcg gccggcccgt caaggtcttc gaccccgctc
      721 agcgcgcgca gcagccgccg caccgcatgg aacgggatgt cgctggcatg ggattcgcag
      781 aacgcccaca ccacctcgaa cccacgactt gccgccagtg ctgcggcctc ccgcgccgcc
      841 cgggacttgc cgatgcccgg ctctcccacc acattcacca caccaccacg gccaccgagc
      901 gcgcggtcca ccaaggcttc gagggccgcc atctccgaat gtcgaccgac cagacttgct
      961 tcctggcgct gcctcacatg gccggaagtc atgccaagca accggcgtgc ggggacgggc
     1021 gcatcggccc ccttgatgtg cacccattcc ggttcaccca acacagcggc atgttcgacc
     1081 agccgcgcgg tcgactccga cagcatcacg ccggtaggcg ccgcgaccga ttccatccgc
     1141 tgcgccatcc cgacctgctc accgaccgcg gtgtaaccca gtgagcctga accaatctca
     1201 cccgtaatga cctgaccgga gttgagcccg acgcgcagct gaaaggagat gccatcacgg
     1261 cgctcgacgt cggcggccag acgtacagct tctttctgga catccagggc cgccagacat
     1321 gcccgcacgg cgtggtcctc caacgccacc ggcgcgccga acaacgccat gatcccgtcg
     1381 ccggtgaact tgtcgaccgt gccgccaaag cgcagcacca ccgccgcgca ccggttcacc
     1441 aactcggtca tgatctcgcg caaccgctcg atatccagcg cagccgccat atccatcgaa
     1501 cgcaccacat cggcgaacag caccgtcacc tgcttgtact ccgcagcatc ggccgatgcc
     1561 accagcggcg acccgcacgc atcacagaat cgagcaccct catgcggcgc agccccacac
     1621 gaccggcaaa cctctcctgc cggcgtcgct ggcgttgtcg ctcccgcggc ccccgctgac
     1681 tgcacgatca gcaaagcgta tggcttcatg cgcgcacaaa cggtcaaacg gacgatattg
     1741 acgtctgcgg cacaaacggg agccgcaacg agcagtggaa tggctgagtt acgttgcttc
     1801 ggtgagtaat accggacccc tcgccggggt gaaggtgatc gaactcggcg gcatcggccc
     1861 cgggccgcac gcagcgatgg tgctggcgga tcttggcgcg gacgtggtgc gggtgcgcag
     1921 gccgggcgga ttgcagatcc cagcggaaaa cctcgacctg ttgcatcgcg gcaagcgcat
     1981 cgtcgacctt gacgtcaaga accagcggga caagctgctg gagctggtgg ccaaggccga
     2041 cgtgttgctc gacgggttcc ggcccggcac ctgcgagcgg ctcggcatcg ggcccgatga
     2101 gtgcgcggcg gtcaacccgc ggctgatcta tgcgcgcatc acgggctggg gtcaggacgg
     2161 cccgatggcg aaggccgccg gtcacgacat caactacctg tcgcagaccg gggcgctctc
     2221 ggcgatcggc tatcgcgacc ggccaccggt ggccccgctg aacctggtcg ccgacttcgg
     2281 cggcggatcg atgttcgtgc tgctcggcat cgtcgccgcg ctctacgaac gggaacggtc
     2341 gggcaagggc caggtgatcg acgccgcgat ggtcgacggg gtgtccatcc tggcgcagat
     2401 ggcgtggacg atgaagtcga ccggttcgct caaagacgag cgcgaatcgt tcatgctcga
     2461 cggcgg
//
LOCUS       DASRJA010000764         8998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3498601,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000764 DASRJA010000000
VERSION     DASRJA010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8998
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3498601"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VFC01_34545"
     CDS             complement(<1..622)
                     /locus_tag="VFC01_34545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZN84775.1"
                     /translation="MRTQRKYWSLWSPTRSFRSLAGQFLVFQLLVVAVVLVAVAAVSV
                     AQSTREFREVRGQRMIAVAENMASTPIVRDRYADPFATRVLAPEVDRAVALSGAGLAE
                     ILNPDGHVRVSSDPSRIGQRMDLGPSRADEGRAWFGDDNIDRVHSLVGQVPILSTNGD
                     VLAVVSVSEPYPSVWELLSGAGERLLIYLSLGAALGLLASWLLSRRI"
     gene            674..1669
                     /locus_tag="VFC01_34550"
     CDS             674..1669
                     /locus_tag="VFC01_34550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZN84776.1"
                     /translation="MIGDDVKSTQQRAALILVVIAALLLTACGVTKGEQTGLHRLRMM
                     VPNSPGGGYDLTARTAVKIMEDDDITGRVEVFNVIGAGGTVAMARLMNEKGNDDLMMT
                     MGLGVVGATYTNGSKARASDATALAKLIEDPGAVFVPADSPFKTVQDFVAAWKADPSK
                     VTIGGGSSPGGPDHLFPMELAKAVGVEPKSVNYVTYDGGGDLLTALLGKKITVGTSSP
                     GELMDQIEAGQLRVLAVSGAERVEGINAPTLKESGINLTFANWRGVLAPPDISDDAKQ
                     AMVKVLEEMHGTKQWKEALVKNGWTDAFVTGQQFEQFLKEQDTRVSETLTELGLV"
     gene            1666..2190
                     /locus_tag="VFC01_34555"
     CDS             1666..2190
                     /locus_tag="VFC01_34555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HZN84777.1"
                     /translation="MTTTEEPQQNPVARTVDKAQYIVCAVLVAVGAFVIYDALSLEAG
                     FAKVDPVGPKFFPIAIGGILIVLAIVLAIAIPRGSVGEADAGEDVDPNTPGDWRTVGL
                     LVGLFVSVILLVNPLGWAITGTLLFAGAATILGSRYYIRNLVIGAVLAVASFYAFYSG
                     LGIPLPAGILDGIL"
     gene            2192..3733
                     /locus_tag="VFC01_34560"
     CDS             2192..3733
                     /locus_tag="VFC01_34560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZN84778.1"
                     /translation="MENLDWLLQGFEQAATPMNLLYAVIGVLLGTAVGVLPGIGPAMT
                     VALLLPVTYNVSPSAAFIMFAGIFYGGMYGGSTTSILLNTPGESSSVITAIEGNKMAK
                     AGRAAQALATAAIGSFVAGAIGTALLAAFAPPISRFAVTLGAPSYLAIMLFALVAVTA
                     VLGASKLRGAISLFLGLAIGIVGIDFLTGQPRATFGLPQLSDGIDIVVIAVAIFAVGE
                     ALWVAAHLRRRPADVIPVGRPWMGREDWRRSWKPWLRGTAYGFPFGALPAGGAELPTF
                     LSYITEKKLSKHKGEFGRGAIEGVAGPEAANNASAAGTLVPMLSLGLPTNATAAVMLT
                     AFVSYGIQPGPTLFEKEPLLIWTLIASLFIGNFMLLVLNLPLAPLWAKLLRTPRPYLY
                     AGILFFATLGALAVNVQPLDLALLLVFGLLGLMMRRFGLPVLPLIIGVILGPRIERQL
                     RQALQLGGGDWISLFKEPVAITVYVLMAALLLVPLVLRLMHRSEETLLVVEDDRDQKE
                     KAAQA"
     gene            3837..4229
                     /locus_tag="VFC01_34565"
     CDS             3837..4229
                     /locus_tag="VFC01_34565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZN84779.1"
                     /translation="MIVVGYSTDPFGRAALEHGIAEAKRRGTTLCVINSTSGDAYVDT
                     HFAQPGEVHDIEERLANCGVEFELTQPVGVDTAEELLKAMDRDDAELLVIGIRHRSPV
                     GKLLLGSVSQQLLLECPKPVLAVKPDEL"
     gene            4239..5375
                     /gene="cax"
                     /locus_tag="VFC01_34570"
     CDS             4239..5375
                     /gene="cax"
                     /locus_tag="VFC01_34570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HZN84780.1"
                     /translation="MEVEAPPAERRKPFLLTGEGWPYLLVPLIPVAIVLEFMHADAVL
                     VFAASALGVIPTAALMGRATEELAARSGPGLGALLNVTFGNAPELIIALFALGAGLHE
                     VVKASLVGSILGNILLVLGMSMLAGGLKRERQRFELRAATAQSLMLLLATVALVMPAI
                     FQLVAGGGLPEPRARAVDFSSDLTALSVGVAVVLLASYAAGLVFSLKTHAHLFNPYDE
                     ESDHGGEPWSVRKSVLMLAVAGAAVGWMSEILVHSITEASDAIGLSPFFVGVIVVAVV
                     GNAAEHWVAVYFAARDKMDISINIAIGSAAQIALFVAPVLVLASFVIGPFPMALVFNG
                     FEIGAIFLAIIIANEVTQRGESTWYEGLQLLAIYAVLGLTFYFV"
     gene            complement(5377..6201)
                     /locus_tag="VFC01_34575"
     CDS             complement(5377..6201)
                     /locus_tag="VFC01_34575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014985913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN84781.1"
                     /translation="MDLTERFKWQGRQIAWGRAGDGPAVVFCHGTPWSSVLWRPIADA
                     LTARYSVYLWDMPGYGRSSMHPDHPVDFGVQADAFNALLRHWDIARPHVIAHDFGGAV
                     SLRAHLVGGADYASLMLIDVVAIPPSGSPFFKFVQDNPGLLDQLPGYVHRAIVRAYIG
                     NASSSGLRDEDLDNLVNPWTGEEGQKAFYRQIVDYDERYLRENEELLPKVTVPVHIVW
                     GEQDAWIPVERADRLHTLMRHSTLCKVPGSNHLIQFDAPERLMYEVTSWLERVSGA"
     gene            complement(6248..6562)
                     /locus_tag="VFC01_34580"
     CDS             complement(6248..6562)
                     /locus_tag="VFC01_34580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011560615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN84782.1"
                     /translation="MSEVVLGPVDEIPIGEGRTFAVDGEQIAVFRLRDGSLRAIEAVC
                     PHKGGPLADGLADDRVVVCPLHGHTFDMCTGAEAGGADMSVRSYSVSAVDGAIRITHH
                     TM"
     gene            complement(6559..>8998)
                     /gene="nirB"
                     /locus_tag="VFC01_34585"
     CDS             complement(6559..>8998)
                     /gene="nirB"
                     /locus_tag="VFC01_34585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HZN84783.1"
                     /translation="NGMAGARLVEDVLARTSGDRFKITMFGDEPYGNYNRILLSNVLA
                     GSDHADEIYLNGLDWYADNDIDLRAGVRVVRIDAFARLVHADDGSVTRYDYLILATGS
                     RSFFPPMKGLWADNKSLIDGVFGFRTLDDCLAMIDGAPERTKAVVIGGGLLGLEAARG
                     LQNRGLTVEVVHAAPTLMNAQLDDSAGAILRKSVEGIGISVHVQKVTTEVLSADGRLT
                     GIVFNDGSSIDCDMLVIAAGIRPNVGLAQRAGLTVERAIVVDDHMRSIDDDSIYVVGE
                     CAQHRGQVYGLVAPLWEQAAVLADHVTGADRTAAYHGSRTATKLKVAGVDVAAMGLKA
                     PETDDDEFVQYSEPRHGVYKTIVIRDGKLVGATLVGDVSKVSFLTQAFDSGLPLPEER
                     VSLMFDIGTPDVSVGVAELSDDAQVCNCNGVSKGTLVACVQGGETSVTGVMAKTKAGK
                     GCGSCKELVGQIVEWAADGAITEDPSASWYVPGIPYDKPTLMQHIRELKLHSVSSVFA
                     ALAPDGQEDANSKMALASLLEMMWADEFVDERDARFINDRVHANIQRDGTFSVVPQMK
                     GGVTNSWQLRRIAEVADKYAIPMIKLTGGQRIDLLGVRKEDLPAVWADLDMPSGYAYG
                     KSFRTVKTCVGSDFCRYGLGDSTALGIALEERYQGLASPAKMKLAVTGCPRNCAEALC
                     KDLGVVAVGDGRWEIYVGGAAGAHIRKGDLLATADDPDTVITLTGRFLQYYRENANWL
                     ERTYKFVPRVGIDKIRAVVVEDAEGIAEQLDTSMQKSVDAYRDPWNDGREPVTEGQFR
                     TSLPLLPLPQVPVR"
BASE COUNT         1562 a         2965 c         2839 g         1632 t
ORIGIN      
        1 tgatccgtcg cgacaacagc cacgacgcca gcagccccag cgctgcacca aggctgagat
       61 agatgagtag ccgctcgccg gcgccgctga gcagttccca caccgacggg tacggctcgc
      121 tgaccgatac gacggccaac acgtcaccat tggtggaaag gatcggcacc tggccgacca
      181 agctgtgcac acggtcgatg ttgtcgtcgc caaaccaagc ccggccttcg tcggcccggc
      241 tcggcccgag gtccatccgc tggccgatgc gagacggatc cgacgacacc cgcacatgtc
      301 catccgggtt gaggatctcg gccagccccg cacccgagag cgcaacggcg cggtccacct
      361 ccggcgcaag cacccgcgtg gcaaatggat cggcgtagcg atcccgcacg atcggtgtcg
      421 acgccatgtt ttcggcgacc gcgatcatcc gctgtccccg cacctcgcgg aactcccgcg
      481 tcgactgcgc caccgacacc gccgccacgg cgaccagcac gacggcaacc accagcaact
      541 ggaacaccag aaactggccc gccaggctgc ggaaactacg agttggtgac cacaacgacc
      601 aatacttcct ttgcgttctt atcagtgacg taggtcactg cagacgtcca gtattgctga
      661 gcatcacaaa tcagtgattg gggacgacgt gaaatcaacg cagcaacgcg cagccctaat
      721 actcgtcgtg atcgccgcgc tcctccttac ggcctgcggc gtgacgaagg gcgaacagac
      781 gggtctgcac cggctccgga tgatggtgcc gaacagcccc ggcggcggct acgacctcac
      841 ggcgcggacc gccgtgaaaa tcatggagga cgacgacatc accggccgcg tcgaggtctt
      901 caacgtcatc ggcgcaggcg gcaccgtcgc catggcccgc ctgatgaacg aaaagggtaa
      961 cgacgacctc atgatgacga tggggctcgg cgtcgtcggc gccacctaca ccaacgggtc
     1021 caaggcgcgc gcatcggacg cgacggcgct ggcgaagctg atcgaggacc ccggcgcggt
     1081 tttcgtcccg gccgattcgc cgttcaagac ggtgcaggat ttcgtcgcgg cgtggaaggc
     1141 cgatccgtcg aaggtcacca tcggcggcgg ttcgtcaccg ggcggacccg atcacttgtt
     1201 cccaatggaa ttggcgaaag cggtcggtgt cgagccgaaa tcggtcaact acgtcacata
     1261 tgacggtggc ggtgatctgt tgaccgcgct actcggcaag aagatcaccg tcggcacctc
     1321 cagcccgggt gaactcatgg atcagatcga agccggccaa ctccgggtgc tcgccgtatc
     1381 cggggcggag cgagtcgagg gcatcaacgc acccaccctc aaggagtccg gcatcaatct
     1441 caccttcgcg aattggcgcg gagtccttgc gccaccggac atctccgatg acgcgaaaca
     1501 agcgatggtc aaggtgctcg aagagatgca cggcaccaag cagtggaagg aggcgctggt
     1561 gaagaacggc tggactgatg cgttcgtgac gggccagcag ttcgagcagt tcctcaagga
     1621 gcaagacacc cgggtctcgg agaccctgac cgaattgggg ctggtatgac caccaccgaa
     1681 gaaccgcaac agaatccggt tgccagaaca gtcgacaagg cgcagtacat cgtctgtgcg
     1741 gtgctggtcg cggtcggcgc attcgtgatc tacgacgcgc tcagcctgga ggcgggattc
     1801 gcgaaggtgg atcccgtcgg gcccaagttc tttccgatcg cgatcggcgg catcctcatc
     1861 gtgctcgcaa tcgttctcgc cattgccatt ccgcgcggct cggtgggtga ggcggacgcg
     1921 ggggaggacg tcgacccgaa caccccgggc gactggcgca cggtcggact gctcgtcggt
     1981 ctgttcgtgt ccgtgatcct gctcgtcaac ccactcgggt gggcgatcac cgggactctg
     2041 ctgttcgcag gtgcggcgac catcctcggc agccgctatt acattcgcaa cctcgtcatc
     2101 ggcgcggtgc tcgccgtcgc cagcttctac gcgttctact ccgggctcgg aatcccgttg
     2161 cccgcaggca ttctcgacgg gatcctctga catggaaaac ttggactggc tgctccaggg
     2221 cttcgagcag gccgcgacgc cgatgaatct gctgtacgcc gtcatcggcg tactcctcgg
     2281 gaccgctgtc ggtgtgctcc ctggcatcgg ccctgccatg accgtcgcac tcctgttgcc
     2341 cgtcacctac aacgtgagtc ccagcgcggc gttcatcatg ttcgccggca tcttctacgg
     2401 cggcatgtat ggcgggtcga ccacgtcgat cctgttgaac acgccgggtg aatcgtcgtc
     2461 ggtcatcacg gcgatcgaag gcaacaagat ggccaaggcc ggcagggcgg cgcaggcgct
     2521 tgcgacagcg gccatcggat cgttcgtcgc cggcgcgata ggcacggcct tgttggccgc
     2581 attcgcgcca cccatctcca gattcgcggt gacgctgggc gctccgtcct acctggcgat
     2641 catgctgttc gccctcgtcg cggtgacggc ggtgctcggt gcctccaagc tgcgcggcgc
     2701 gatatcacta ttcctcggcc tcgcgattgg catcgtcggc atcgacttcc tcacgggtca
     2761 gccccgggcg acgttcggcc ttccgcagct gtccgacggg atcgacatcg tggtgatcgc
     2821 ggtcgccatc ttcgccgtcg gcgaggcgct ctgggtggcc gcccatctgc gccgccgtcc
     2881 ggccgacgtc attccggtgg gtcggccgtg gatgggccgc gaagactggc ggcggtcgtg
     2941 gaagccgtgg ctgcgtggca ccgcttacgg gttcccgttc ggcgcactgc cggccggcgg
     3001 cgccgagctg ccgacgttct tgtcgtacat caccgagaag aagctgtcca agcacaaggg
     3061 cgaattcggc aggggtgcga tcgagggcgt cgcgggacct gaggccgcca acaacgcctc
     3121 ggccgcaggc acattggtgc cgatgctgtc gctcgggttg cccaccaacg ctacggcggc
     3181 ggtgatgctg accgcgtttg tgtcctacgg gatccagccc ggcccaacgc ttttcgagaa
     3241 agagccgctg ctgatctgga cgcttattgc cagcctgttc atcggcaact tcatgctgct
     3301 ggtgctcaac ctgccgctgg cgcccctgtg ggccaagctg cttcgcaccc cgcgacccta
     3361 cctctacgcg ggcatcctgt tcttcgcgac gttgggcgcg ctcgcggtga atgtgcaacc
     3421 gctcgacctg gcgctgctgc tggtcttcgg tctgctcggg ctgatgatgc gaaggttcgg
     3481 cttgccggtg ctgccgctga tcatcggcgt gattctcggc ccacgtatcg agcgtcagct
     3541 gcgacaggcc ctgcaactcg gtggcggtga ctggataagc ctgttcaaag aacccgtcgc
     3601 gatcaccgtg tacgtgctga tggctgccct tctcctggtg ccgctcgtgc tgcgactgat
     3661 gcatcgcagc gaagaaacgc tcctcgtcgt cgaggacgac cgggaccaga aagagaaggc
     3721 ggcccaggca tgatcacccg aagacgacaa cgagtgagta tcgcagcgag gaacgagcga
     3781 ggaccggagc gcggtggggg tacctcccgc ccgcagggta gggggagagt cgaggcatga
     3841 tcgttgtcgg atacagcacg gacccgttcg gacgggcggc actcgagcac ggcatcgccg
     3901 aagccaagcg gcgcggcacc acgctgtgtg tcatcaactc gacctctggc gacgcctacg
     3961 tcgatactca cttcgcccaa cccggtgagg tacacgacat cgaggagcgg ctcgcgaact
     4021 gcggcgtaga gttcgagctc acccagcccg tcggggtcga taccgccgag gaactgctaa
     4081 aagcgatgga ccgcgacgac gccgagctgc tggtgatcgg catccggcac cgcagccctg
     4141 tgggaaagct gctgctcggc agcgtgtcgc agcagctgct gctcgaatgc ccaaaaccgg
     4201 tgctggcggt caagcccgac gagctgtaag aacggcgcat ggaagttgag gcgccacccg
     4261 ccgaacgtcg taagccattc ctgctgaccg gcgagggctg gccatacctg ctcgtgccgt
     4321 tgatcccggt cgcgatcgtc ctggagttca tgcacgccga cgcggttctc gtgttcgcag
     4381 cttcggcgtt gggtgtgatc ccgaccgcgg cgctgatggg ccgagcgacc gaggaactgg
     4441 cggcgcgttc gggtcccggg ttgggtgcgc tgctcaacgt gacgttcggc aacgccccag
     4501 agttgatcat cgcgttgttc gcactgggcg ccggtctgca cgaggtggtg aaggcctcgc
     4561 tcgtcgggtc gatcctgggc aacatcctgc tggtcctcgg gatgtcgatg ctggcgggcg
     4621 gtctgaaacg ggagcggcag cgcttcgagc tgcgcgcggc caccgcccag tccctcatgc
     4681 tgctgctggc gacggtagcc ctggtgatgc cggcgatctt ccagctggtg gccggcggcg
     4741 ggttgcccga gccgagggcc cgggcggtgg atttctcgag cgacctcacg gcgctgtcgg
     4801 tgggggtcgc cgtggtgctg ctggcctcat acgcggccgg gttggtgttc tcgctgaaga
     4861 ctcatgcgca cctgttcaac ccctacgacg aagaatccga tcacggcggc gagccgtggt
     4921 cggtgcgcaa gagcgtgctc atgctggccg ttgcgggggc cgcggttggc tggatgtcgg
     4981 aaatcttggt ccactccatc accgaggcat cggacgcgat cggactgtcc ccgttcttcg
     5041 tcggcgtcat cgtcgtggcg gtggtcggca acgccgccga acactgggtc gcggtgtatt
     5101 tcgccgctcg cgacaagatg gacatctcca tcaacattgc gattggatct gccgcgcaga
     5161 tagcactgtt cgttgcgcca gtgctggtgc tcgcatcatt tgtcatcggc ccattcccaa
     5221 tggccttggt gttcaacggg ttcgagatcg gcgcgatctt cttggcgatc atcatcgcca
     5281 acgaggtgac ccaacgcggc gagtccacgt ggtacgaagg cctgcagcta ctcgccatct
     5341 acgccgtgct gggactgacc ttctacttcg tctgactcac gcgcctgata cccgctcaag
     5401 ccaggacgtc acctcgtaca tcagtcgttc gggagcgtcg aactgaatca agtggttgct
     5461 gcctggcacc ttgcacagcg tcgaatgccg catgagcgtg tgcagtcgat ccgcccgctc
     5521 gacgggtatc catgcgtctt gctcacccca cacgatgtga accggcacag tgactttcgg
     5581 caacagctct tcgttttcgc gcagatagcg ctcgtcataa tcgacgatct gtcggtagaa
     5641 cgccttctgg ccctcttcgc cggtccacgg gttcaccaag ttgtcaagat cttcgtcacg
     5701 caacccgcta ctgcttgcat tgccgatata ggcgcgaacg atcgcgcggt gaacgtaacc
     5761 cggaagttgg tccagcagac cggggttgtc ctggacaaac ttgaagaacg gtgacccgct
     5821 cggggggatt gccacgacat cgatcagcat gagtgaggca tagtcagcgc cgcccacgag
     5881 gtgggcgcga agcgacacgg cgcccccgaa atcgtgagcg atcacatgag ggcgggcaat
     5941 gtcccagtgc cgtaacagcg cattgaatgc atctgcttga actccgaagt cgacggggtg
     6001 gtctggatgc atcgacgacc ggccgtatcc cggcatgtcc cacagataga cggaatatct
     6061 agcggtcaga gcgtcggcga tcggtcgcca cagcactgac gaccacggag tgccatggca
     6121 gaacaccacg gccggaccgt cgccggcccg tccccacgca atctgacgcc cctgccactt
     6181 gaatcgctca gtcaggtcca cattcccagt gttaccccac cagtgaacgg gctgacaaag
     6241 tctcgactta catcgtgtga tgcgtgattc ggatggcgcc gtcgacagct gacaccgagt
     6301 agctgcgcac tgacatgtcg gctccaccgg cttcggctcc ggtgcacatg tcgaacgtgt
     6361 ggccgtgcag cgggcacacc accacccggt cgtcggctag cccgtcggcc agcggcccgc
     6421 ccttgtgcgg acataccgct tcgatggccc gcaacgagcc gtcccgcagc cggaacaccg
     6481 cgatctgctc accgtcgacg gcgaacgtgc ggccttcgcc gatcgggatc tcgtcgactg
     6541 ggcccagcac cacctcgctc atcggaccgg cacctgcggc agtggcagca gcggcagcga
     6601 tgtccggaac tgcccttctg tcacgggttc tcgtccgtca ttccacggat cccgataggc
     6661 gtcgaccgac ttctgcatgc ttgtatcgag ctgttcggcg atgccctcgg cgtcctcgac
     6721 cactacggcg cggatcttgt cgatgccgac ccggggaacg aacttgtagg tgcgctcaag
     6781 ccaattcgcg ttctcccggt agtactgcag gaaccggcca gtgagcgtga tgacggtgtc
     6841 ggggtcatcg gccgtcgcaa gcaggtcgcc cttgcggatg tgagcgcccg ccgcgccgcc
     6901 gacgtagatt tcccagcgcc cgtcgcccac ggcgacgaca ccgaggtcct tgcacaacgc
     6961 ctcggcgcag ttgcgcggac aacccgtgac ggccagcttc atcttggccg ggctggccag
     7021 gccttggtag cgctcttcga gcgcgatgcc gagcgcggtg gaatcgccaa ggccgtagcg
     7081 gcagaagtcg ctgccgacac aggttttcac ggtccggaag ctcttgccat atgcgtagcc
     7141 cgacggcatg tccagatccg cccacaccgc gggcaggtcc tccttgcgca ccccgagcag
     7201 atcgatgcgt tgcccgccgg tgagtttgat catcgggatg gcgtacttgt cggcgacctc
     7261 tgcaatacgg cgcagttgcc atgaattcgt tacgccgccc ttcatctgcg gcaccaccga
     7321 gaacgtgccg tcgcgctgaa tgttggcgtg cacccggtcg ttgatgaacc gggcgtcgcg
     7381 ttcgtcgacg aactcgtcgg cccacatcat ctccaacaac gatgccagtg ccatcttcga
     7441 gttggcgtcc tcctgcccgt ccggtgcgag cgcggcgaac accgacgaca ccgaatgcag
     7501 tttgagttcg cgaatgtgct gcatcaaagt tggcttgtca taaggtattc cgggcacata
     7561 ccacgacgcc gacggatcct cggtgatcgc cccgtcggcc gcccactcca cgatctggcc
     7621 gacgagctcc ttgcacgacc cgcaaccctt gccggccttg gtcttcgcca tcacgccggt
     7681 gaccgatgtc tcgccgcctt gcacgcacgc caccagcgtg cccttgctca cgccgttgca
     7741 gttgcacacc tgcgcgtcgt cggagagttc agccacgccg acggacacgt cgggggtacc
     7801 gatgtcgaac atcaacgaca cccgctcctc gggcagcggc agcccgctgt cgaacgcctg
     7861 tgtcaggaac gacaccttgc tgacatcgcc gaccagcgtt gccccaacga gctttccatc
     7921 gcggatgacg atcgtcttgt acacgccgtg ccggggctcg gagtattgga cgaattcgtc
     7981 gtcgtcggtt tccggcgcct tcaagcccat cgcggcgacg tcgacgccgg cgaccttgag
     8041 tttggtggcc gtgcgtgagc cgtgatacgc ggcggtgcgg tcggcgccgg tgacatggtc
     8101 ggcgagcacg gcggcctgct cccacagcgg cgccaccagg ccgtaaacct gacctcggtg
     8161 ctgggcgcac tcgccgacga cgtagatgga gtcgtcgtcg atcgaccgca tgtggtcatc
     8221 gacgacgatc gcccgctcca cggtcaggcc ggctcgttgt gccagaccga cattgggccg
     8281 aatgcccgcc gcgatcacca gcatgtcgca gtcgatcgaa ctgccgtcgt tgaacacgat
     8341 cccggtgagc ctgccgtcgg cgctaaggac ttccgtcgtg accttctgga cgtgcaccga
     8401 gattccgatg ccttccaccg acttgcgcag tatcgccccg gcggaatcgt cgagttgcgc
     8461 gttcatcaac gtcggcgccg cgtgaacgac ctccacggtg aggccgcggt tctgcagacc
     8521 gcgagcggcc tcaagcccga gcaggccgcc accgatgacg acggccttcg tccgttccgg
     8581 tgccccgtcg atcatcgcca aacagtcgtc gagagtccgg aacccgaaca cgccgtcgat
     8641 caagctcttg ttgtcggccc acaagccctt catcggcggg aagaacgacc ggctgcccgt
     8701 ggccagaatg agatagtcgt accgggtgac cgacccgtca tcggcatgca ccagccgcgc
     8761 aaacgcgtcg atgcgcacca cccgtacccc ggcacgcagg tcgatgtcgt tgtcggcgta
     8821 ccagtccaga ccattgaggt agatctcgtc cgcatgatca ctgccggcca acacattcga
     8881 caacaggatg cggttgtagt tgccgtacgg ctcgtcgccg aacatggtga tcttgaagcg
     8941 atcgccgctg gtgcgggcga gcacgtcctc gacgaggcgg gcacccgcca tgccgttg
//
LOCUS       DASRJA010000765         9988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_966637,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000765 DASRJA010000000
VERSION     DASRJA010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9988
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966637"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VFC01_34590"
     CDS             <1..986
                     /locus_tag="VFC01_34590"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZN84784.1"
                     /translation="DLASFCALRVLDFDDALRWQRWGRQYHQRIAGTLSVSYGYCFAA
                     LAANEQLDVAGAEAHLRHARRIALLPSGRPTYVAKLAGALLGELLYERGQLDQSEMLL
                     DDAYELGAEGGIVDFMLAAFGTGARLKSARGDVAAAQRRLVEGLDIARTLQLPRLEAR
                     LLNESVRVAARSGEAIDDSVAQRITRSGSAQFDGIGVVAAELMEDARIRLLLIDGTPS
                     ALSDACKRARARVDQLDRSKRPRAHLHATLQLALSLGTAGKPEDAHHYLSAALRTCAA
                     LGLSQLLIDEGPQLLRLANDAVDGKDFNAADPMTSANVRDFVSNLAERSNV"
     gene            complement(998..1378)
                     /locus_tag="VFC01_34595"
     CDS             complement(998..1378)
                     /locus_tag="VFC01_34595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HZN84785.1"
                     /translation="MSSSTSTNIRHAFFLLAGAVALVLTWPHAFQWIADGGNILNPVD
                     FFGDAIAAGGTAAFLSIDMAIAWVAFMVWVVTDANRIGLGYKWGWLFVALSYIGVSMA
                     FPVYLVTRERYLDKRARTDELSTA"
     gene            complement(1390..2190)
                     /locus_tag="VFC01_34600"
     CDS             complement(1390..2190)
                     /locus_tag="VFC01_34600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84786.1"
                     /translation="MPPVDHELNLTMQAILTDVSWGLTAVLLVIAIWMGMRERTPFYA
                     LIVLAAAVGAFAEPLYDVAMMLYFYSTEGMWTHFTAFDIPQPIWTHSGYVVLYAAPAI
                     YIAHQIARGTMTRRKLLVWAGITLLESAAFEMIGINGGLYSYWGPHVFRIFQYPLVIG
                     VLEAAQVICFAVAASQLRARCTGRWQLLGLFVLFPFTFFGANFGAGWPTIIAIHLEHT
                     STALVAAATVVSIGFAAVLVRGAAAFLPQRTAAAGADDRAHNHAPAKL"
     gene            2515..3648
                     /locus_tag="VFC01_34605"
     CDS             2515..3648
                     /locus_tag="VFC01_34605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN84787.1"
                     /translation="MFTPDQLEFAAAIEQFCEKHCATAQQRDALTDGGRLSNSPEILS
                     RFAELGWLGVSLPVEYGGGGAGMVEECIFLEETARGLAPIHAYGTGLTAAQTYLRHGD
                     EQQKKEVLGNLCSGHFEAIALSEPGAGSDLSGVTTKAVRDGDRFVVNGQKTWITAAHL
                     ADHLLVLTRTDPTAGKHNGLTLLYLPTSSPGLDVRPIQTMDGHTVNDVFFTDVSVPVA
                     NVVGGIGDAWHRLMRGLNVERLIIAAMSLGAARRSLEDTIAYVRTREQFGKPIGTFQA
                     VRHTIADLATEIEYCRAFAYHVAAQIDAGLEDQLAREGSMAKLKCTEVAKMVALEGLQ
                     LMGGNGYTVEFGMEKQVRKALAPPIYGGANAIQREIIGKSLGL"
     gene            3669..4574
                     /locus_tag="VFC01_34610"
     CDS             3669..4574
                     /locus_tag="VFC01_34610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN84788.1"
                     /translation="MSATTRDIFAPDLLSDKRILITGGGTGLGRGVAARLVKHGAEVH
                     LWGRREAVLAEAAQEASAARPGAAHYQAVDVRDSASVEAAVGEIWERHGPLTGLLNNA
                     AANFIAPTATLSQRAFEAVTSTVMTGSFNTTVAVGKRWIAAGLPGAVLSNLTTWVWSG
                     SAFVVPSAMAKAAVHAMTMSLAVEWGRYGIRVNALAPGPIPTEYAWEMLNPTDKSAVG
                     ATQIDQIPLGRSGTIDELANLTIFAFSDACDYLTGETIAMDGGQRLAGPNTFAGLTAM
                     SDADWAEARERSKAASAAAKSARSV"
     gene            complement(4588..5598)
                     /locus_tag="VFC01_34615"
     CDS             complement(4588..5598)
                     /locus_tag="VFC01_34615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HZN84789.1"
                     /translation="MWAWSDTELERLGSFLSTHGLCGRSVTAAAIGDGHSNLTFLVSD
                     GHSRVVVRRPPPPPLPPGAHDVLREARLLTALAPMDVPSPRVLATAPAGELLDVPVLV
                     MDFVDGAVITEKTPAPRDDEGLRRRIAESLVDTLAALHAVPWREVGLGDFGKPEGFNA
                     RQLRRMRSLVAVDETVPKPFAVLDDWLHAKLPSESGTSIVHNDFRIGNMIVDPGTGRI
                     AAVLDWELATIGDPLADLGYLLTSYPVPGEPLVPTAAMGTAVLEPGYPGRKELVDRYA
                     DRTGADVSNVNWYATLAMYKLAALYEYSRRRFEEGVGDPYYADPALVASFLDAGERLA
                     LA"
     gene            complement(5598..6530)
                     /locus_tag="VFC01_34620"
     CDS             complement(5598..6530)
                     /locus_tag="VFC01_34620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84790.1"
                     /translation="MTAARERSSGLRPTDDLRHRPVAGERTRDSLFWQLTMPDHELGV
                     QVYLYLTGTGKAGYNVCVWGPDAQPLAMHLHSGQVPDHADLDEFSFDGLTLIQPELRK
                     SCLMTYDRDDVRIEFAFTGIHDAFSYRCNPDGLPSWFAVNRIEQTGRVRGGIEVAGRR
                     IEWDRMGHRDHSWGVRDWGVPQHWKWFVAYTESGRAINGWTWIAKGEWGFAGYVVRDG
                     VTYAVSNIDQHADYGAQMRQRRLRATLTDVTGSETELELDVFGVIELPTHDRLQTVIR
                     EGACRAVIDGEEGAGQFETHWQGGYLDYLSRQGS"
     gene            complement(6527..7336)
                     /locus_tag="VFC01_34625"
     CDS             complement(6527..7336)
                     /locus_tag="VFC01_34625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN84791.1"
                     /translation="MSSITTQWNRVSRRPLADVLDELRTARTLAFDDRGDGIVVMAIN
                     RPERANSQTVEMFGEIAWAAATLRNAPLRALIVTGAGGKAFCTGFDLGEVDVLTRMSV
                     PEFTDLIETAGSMSTGFRALPYPVVAAVSGPAAGGGLSLALAADIRIADETASFSAGF
                     VRIGLSIGELGTSWNLIRLIGPGRAAEVAFTGRTVRAAEAVRIGLADRLIETATALDA
                     AIELARAIAANSDDNVRSTKNALVRNLENNSFASALEMDNRGQALAQLGAR"
     gene            complement(7339..8151)
                     /locus_tag="VFC01_34630"
     CDS             complement(7339..8151)
                     /locus_tag="VFC01_34630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006372188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN84792.1"
                     /translation="MIDLEQRYKTLLLESPADGLVQVTLNRPERLNAMTVGMFDELES
                     VARAVGDDRDIRVVIITGAGKAFCAGYDLDDAEELASLSALGMLDRQERAARAVSALR
                     ALPVPVIAAVNGPAAGGGLSLALMADIRLGSPSAKFNAAFVRIGLSAGDLGASWILPR
                     LIGPSAAAEIGFTGRMVEAEEAERLGLLNRVTGEDDLLDQTLTMAGQICANSPGGIRI
                     SKRALQANMEIASFAAALELENRGQALLTRCDDMTEALEAFKAKRAPNFSGR"
     gene            complement(8167..8925)
                     /locus_tag="VFC01_34635"
     CDS             complement(8167..8925)
                     /locus_tag="VFC01_34635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN84793.1"
                     /translation="MTWNRHGAIVEFVLDRPPANALGLEIIDGLHTAIDYAEAAEVKV
                     IVLASAIGGFFAAGADIKHMSSADAASFRNYGDALRGVLERLAALPMMSVAAIEGLAL
                     GGGLELAMACTLRVAAPKARLGLPEVKLGLIPGGGGTQRLPRLVGRSRALDIMLTGRQ
                     VEADEAFWIGLVDRLAEPGDTARAAAWQLAHELAAASTPAQLAVVRTVDAAYDRPLAD
                     GLRYEVDQIQELFERGEAVEGLQAFIDKRAPEFV"
     gene            complement(8986..9744)
                     /locus_tag="VFC01_34640"
     CDS             complement(8986..9744)
                     /locus_tag="VFC01_34640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009475543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HZN84794.1"
                     /translation="MSNRVAFVTGGAQGIGEGISVTLGADGFRVAVADLNLEAAEATA
                     KRITDAGGEAIAVHVDVTDTESVRGAVKTVVAQLGEIEVVVNNAGWDDFMPFLKTSED
                     FWDKILDINFKGALRVIHTVVPGMVERGFGRVINIGSDAGRVGSSLEAVYSGAKGGII
                     AFTKTLAREVATEGVTVNTVCPGPTDTPALRKFADSSGQDADKVLGGMTRSVPMKRLA
                     QPSDIAAAVAFFASDAAEYITGQTLSVSGGLTMA"
     gene            complement(9779..>9988)
                     /locus_tag="VFC01_34645"
     CDS             complement(9779..>9988)
                     /locus_tag="VFC01_34645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HZN84795.1"
                     /translation="VPAGSTGNIASEDLISMFEEMGVSTGVSLPAIIDAAREAQTVLG
                     RKLTSHSIVAGPIEWKPGPDATSCR"
BASE COUNT         1835 a         3186 c         3267 g         1700 t
ORIGIN      
        1 ccgatctcgc atcattctgc gcgctgcgag tgctggactt cgatgacgcg ctccgctggc
       61 agcggtgggg acgccaatac catcagcgga tcgctggcac cctgagtgtg agctacggat
      121 actgctttgc ggcgctcgcc gcgaacgaac agcttgatgt ggccggtgcc gaagcgcacc
      181 tgcgtcacgc aaggcggatc gcgctgctac cgtccggacg ccccacgtat gtcgcgaagc
      241 tcgccggagc attgttggga gagcttctct acgaacgcgg ccaactcgac caatcggaga
      301 tgttgctcga cgacgcgtat gaattgggcg ccgagggcgg aatcgtcgac ttcatgctcg
      361 cggcgttcgg caccggagcc cggctgaaat cagcgcgcgg tgacgtggcg gccgcgcagc
      421 gcagactcgt cgaaggtctc gatattgcca ggacactgca gcttccgcgg cttgaagccc
      481 ggctgctcaa tgaaagtgtt cgggttgccg cgcggtcggg tgaagcgatc gacgactcgg
      541 tggcacagcg aatcacacgc tcgggttcgg cgcagttcga cggaatcggt gtggtagcag
      601 ccgaactcat ggaagatgca cgaataagat tgcttctgat cgacgggacg ccgtcagcgc
      661 ttagtgacgc ttgtaaacgg gctcgcgctc gcgttgacca acttgatcga agcaagcggc
      721 cgcgtgcaca ccttcatgcc acgctgcagt tggcgctcag cctcggtacc gcgggcaaac
      781 ctgaggatgc tcaccactac ctgagcgcgg cactgagaac ctgtgccgcg ctggggctta
      841 gccaattgct cattgacgaa gggccccagc tgctgcgcct cgctaacgac gccgtggacg
      901 gaaaagactt caacgccgcg gatccgatga cgtcggcgaa tgttcgggat ttcgtttcga
      961 acctcgctga gagatcaaac gtctgacacg tcgcacgtca ggcggtgctc aattcgtcgg
     1021 tccgagcgcg cttgtcgagg taccgctcac gggtgacgag gtatacaggg aaagccatcg
     1081 atacgccgat atacgacaat gcgacgaaga gccagcccca tttgtatccc agtccaatcc
     1141 ggttggcgtc ggtcaccacc cagaccatga acgcgaccca ggcgatagcc atgtcgatgc
     1201 tgaggaacgc ggctgtgccg ccagccgcga tcgcgtcgcc gaagaagtcg acggggttga
     1261 ggatgtttcc accgtcggcg atccactgaa atgcatgcgg ccacgtcaac accagcgcaa
     1321 cggcgccggc aaggaggaaa aacgcgtggc ggatattggt ggacgtggat gaggacactg
     1381 attgattccc tacagcttgg ccggggcgtg gttgtgcgca cgatcgtcgg caccggcggc
     1441 ggctgttcgc tggggcagga acgccgcggc cccccgcaca agcactgccg cgaagccgat
     1501 gctcaccacc gttgccgccg cgaccaatgc cgtcgaggtg tgctcgagat gaatagcgat
     1561 gatggtgggc cagcctgcgc cgaagttggc cccgaagaat gtgaacggaa acaggacgaa
     1621 cagacccaac aattgccacc gaccggtgca gcgcgcacgc agctgcgatg ccgccaccgc
     1681 gaagcagatc acctgagcgg cttcgagcac tccaattacc aacgggtact ggaagatccg
     1741 gaacacgtgt gggccccagt aactgtagag accgccattg atgccgatca tctcgaacgc
     1801 ggccgactca agcagcgtga tgccggccca gacgaggagc ttgcggcggg tcatcgttcc
     1861 gcgggcgatc tgatgggcga tgtagatcgc gggggcggcg tagagcacca catagccgct
     1921 atgcgtccag atcggttgtg ggatgtcgaa cgcggtgaag tgcgtccaca tgccctccgt
     1981 tgagtagaag tacagcatca tcgccacgtc gtagagcggc tcggcgaatg cgccgacggc
     2041 ggcagccagc acaatgaggg cgtagaacgg cgtccgctcg cgcattccca tccagatcgc
     2101 gatgaccaaa aggaccgcgg tcagtcccca gctgacgtcc gtcaggatcg cctgcatggt
     2161 gaggttcagc tcgtgatcga ccggtggcac gccgtcacgg tattgggcaa atggcaccgg
     2221 acgcgtcccc agccagaggt attccacccc tcttacgaga gtgacggttc taagccggca
     2281 tggcatctgc ccgggattgg cccggacgag cagcagaacc gagatcgccg gcatgatcac
     2341 caggaataac gcaatgagta caggattgct gttctcgacg agcgccctct gaatcgacgt
     2401 ccgggctgag tgaaccgtgc accacaaaca tggtcaggag tcgcctaaaa ggcaaatgtt
     2461 tgatatggtc agaccgaata ggctagcttt cgatgacgag aggcccgacg ccggatgttc
     2521 acccccgatc agctggaatt cgccgcggcg atcgagcagt tctgtgagaa gcactgtgcc
     2581 acggcgcaac agcgtgacgc gctcaccgat ggcgggcgac tttcgaacag ccccgaaata
     2641 ctgagcagat tcgccgagct cggctggctc ggggtatcgc taccggtcga atacggcggt
     2701 ggcggcgcgg ggatggtcga ggagtgcatc tttctcgagg agaccgcccg cggcctggca
     2761 ccgatccacg cctacgggac cggattgact gctgcccaga cttatctacg ccacggcgac
     2821 gagcagcaga agaaggaagt actcggcaac ctgtgcagcg gacacttcga ggcgatcgcc
     2881 ctctccgagc ccggcgccgg gtccgacttg agtggtgtga cgaccaaggc ggtgcgtgac
     2941 ggtgatcggt tcgtggtcaa cggtcaaaag acgtggatca ccgccgcgca cctcgccgac
     3001 cacctgctcg tgctgacccg aaccgatccg actgccggca agcacaatgg gctgacgctg
     3061 ctctatctgc cgaccagctc gccggggctc gacgttcgac ccattcagac catggacggc
     3121 cataccgtca atgacgtgtt cttcaccgat gtgtcggtgc cggtggccaa cgtggtcggc
     3181 ggaatcggcg acgcctggca tcgactaatg cgcgggctca atgtcgaacg gctgatcatc
     3241 gcggcgatgt cgctcggtgc ggcccgccga tcgctcgagg acaccatcgc ctatgtgcgc
     3301 acccgcgaac agttcggcaa gccgatcgga accttccagg cggtccggca caccatcgcc
     3361 gatctggcga ccgagatcga atactgccgc gccttcgcct atcacgtcgc ggcgcagatc
     3421 gacgccgggc tggaggatca gttggcccga gaaggttcga tggcgaagct gaagtgcacc
     3481 gaagtggcca aaatggtggc actggagggt cttcagctga tgggcggcaa tggctacacc
     3541 gtggaattcg gcatggagaa gcaggtgcgt aaggcactgg cgcctccaat ctatggtggc
     3601 gccaatgcga ttcagcggga aatcatcggc aagagcctgg gtctgtagct gtgacgaacg
     3661 gagcggccat gagcgcaacg acgcgcgaca tcttcgcacc cgacctgctg tcggataagc
     3721 ggatcctgat caccggcggt ggaacaggtc tcggcagggg ggtggccgcg cgcctggtca
     3781 agcatggcgc cgaggttcac ctctgggggc ggcgcgaagc cgtgctggcc gaggccgccc
     3841 aagaagcctc cgcggcccgc ccgggagcgg cgcattacca ggccgtcgac gtacgggatt
     3901 ccgcctcggt cgaggcggcg gttggggaga tctgggagcg ccacggaccg ctgaccgggc
     3961 tactcaacaa tgccgcggca aatttcatcg ccccgaccgc caccctgagc cagcgtgcct
     4021 tcgaagcggt gacctcgacg gtgatgaccg gatcgttcaa cacaacggtt gccgtgggca
     4081 agcgctggat cgcggccggg ctgccgggcg cagtgctgtc caatctcacc acgtgggtgt
     4141 ggagcgggtc cgcattcgtc gtcccgtcgg ccatggccaa agcggcggtg catgcgatga
     4201 cgatgtcgct ggcggtggag tggggacggt acggcatcag ggtgaatgcg cttgcgcccg
     4261 gaccgattcc gaccgagtac gcgtgggaga tgctcaaccc caccgacaag agcgccgtcg
     4321 gcgccacgca gatcgatcag atcccgctcg gtcgctccgg aaccatcgac gagctggcga
     4381 atctgaccat ctttgcgttc tcggacgcgt gcgactactt gaccggcgag accatcgcga
     4441 tggacggtgg ccagcggctg gcggggccga acaccttcgc cggtctcacc gcgatgagcg
     4501 acgcggattg ggcagaagcc cgtgagcgca gtaaggccgc ctcggccgcc gccaagtcag
     4561 cccgctccgt gtgagtttcg gcgcatatca ggcgagcgcc aggcgttcgc ccgcgtccag
     4621 gaatgacgcg accaacgccg ggtcggcgta atacgggtcg ccgacaccct cttcgaaccg
     4681 cctgcgactg tattcataga gggcggcgag cttgtacatc gccagcgtcg cataccaatt
     4741 gacattggac acgtcggcgc cggtgcggtc ggcgtagcga tcgaccagct ccttccgccc
     4801 cggatagccg ggttccaata cagcggtgcc cattgcagcg gtcggcacca gtggctcacc
     4861 gggcaccgga tacgacgtga ggaggtatcc gaggtcggcg agcggatcgc cgatggtggc
     4921 gagctcccag tccagcaccg cggcgatacg accggttccg gggtcgacga tcatgtttcc
     4981 gatccgaaag tcgttgtgca cgatagatgt tccggactcg gagggcagct ttgcgtgtag
     5041 ccagtcgtcc agtaccgcga acggcttggg taccgtttcg tcaacggcaa ccaaggatcg
     5101 catccgccgc agctgtcgcg cgttgaaccc ctcaggcttg ccgaagtcgc ccagccccac
     5161 ctcacgccac ggcacggcgt gcaacgcggc gagcgtatca accagggatt cggcgatgcg
     5221 tcgccgcagc ccctcgtcgt cgcgcggcgc cggtgtcttc tcggtgatca ccgcaccgtc
     5281 gacgaagtcc atgaccaaca ccggaacgtc gagcagctcg ccggcgggcg cggtggccaa
     5341 tacgcgcgga gacgggacgt ccatcggcgc cagcgcggtc agcagccgcg cctcgcgcag
     5401 cacgtcatgg gcgccgggag gcaatggcgg cggcggtggc ctgcgcacca caacgcgcga
     5461 gtggccgtcg gacaccagga acgtcagatt ggaatggccg tcaccgattg cggcggcggt
     5521 caccgaccgg ccgcacaggc cgtgcgtgga caggaagctg cccagtcgtt cgagctcggt
     5581 gtcggaccag gcccacatca ggaaccctga cgcgacagat aatctaggta gccaccctgc
     5641 caatgggttt cgaattgacc ggcgccctct tcgccgtcaa tgacagctcg gcaggcgcct
     5701 tcgcggatca cggtctgcag ccggtcatgc gtgggaagtt cgatcacgcc aaacacatcg
     5761 agctccagct cggtttcgct gccagtgacg tcggtcaaag tcgcgcgcag tcggcgctgg
     5821 cgcatctgtg cgccgtagtc ggcgtgctgg tcgatgttgc ttacggcgta cgtgaccccg
     5881 tcacgcacga cgtagcccgc gaaaccccac tcgcccttgg cgatccaggt ccatccgttg
     5941 attgcccggc ccgattcggt ataggcgacg aaccatttcc aatgctgcgg cacgccccag
     6001 tcccgcactc cccaggaatg gtcgcgatga cccatccggt cccactcgat gcgccgtccg
     6061 gcgacttcga tcccgccgcg tacccgaccc gtctgctcga tccgattcac cgcgaaccag
     6121 gacggcaaac cgtcaggatt gcagcggtag ctaaacgcgt cgtgaatgcc ggtgaaggcg
     6181 aactcgattc gcacatcgtc gcggtcgtag gtcatcaggc acgacttccg gagctcgggc
     6241 tgaatcagcg tcaatccatc gaacgagaac tcgtccaggt cggcatgatc gggcacctgt
     6301 ccgctgtgca ggtgcatggc cagcggctgt gcgtcgggcc cccaaacgca cacgttgtag
     6361 ccggccttac cagtaccggt gaggtagagg tagacctgca cgccaagctc gtggtccggc
     6421 atggtcagct gccagaacag gctgtcgcgg gtccgttcgc ctgcgaccgg ccggtgccgg
     6481 aggtcgtcgg ttgggcggag tccgctgctc cgctcacgtg ctgcggtcac cgtgcgccga
     6541 gctgcgccag cgcctggccg cgattgtcca tctccaatgc ggacgcaaac gagttgttct
     6601 ccaggttgcg cacgagtgca ttcttggtgg accgcacgtt gtcgtcggaa ttggcggcga
     6661 tggcccgcgc cagttcgatc gccgcgtcaa gggcggtagc tgtttcaatg agccgatcgg
     6721 ccagcccgat gcgcactgcc tcggcggctc gaacggtgcg tccggtgaac gctacctccg
     6781 cggcccggcc cggcccgatc agcctgatca gattccacga tgtgcccagc tcgccgatgg
     6841 acaagccgat ccgcacgaag ccggcgctga aacttgcggt ttcgtcggcg attcggatgt
     6901 cggcagccaa ggccagcgac aggccgccgc ccgcggcagg accgctgacg gctgcgacca
     6961 ccggataagg cagcgcgcga aacccggtcg acatcgatcc ggcggtctcg ataaggtccg
     7021 tgaattccgg caccgacatc cgggtcagaa cgtcaacctc gccaaggtcg aacccggtgc
     7081 agaacgcctt gcctcctgcg cctgtgacga tcagagcccg cagtggcgca ttgcgcaacg
     7141 ttgctgccgc ccaggcgatc tcgccgaaca tctcgacggt ctgcgagttc gcgcgctcgg
     7201 gacgattgat cgccattacc acgatgccgt cgccgcggtc gtcgaacgcc aacgtacgtg
     7261 ccgtgcgcag ttcgtcgagg acgtcggcaa gcgggcgtcg cgacacccga ttccactgtg
     7321 tagtgatgct gctcatgctc acctgcccga aaagttcggt gctcgcttgg ctttgaaggc
     7381 ttccagcgcc tcggtcatgt cgtcgcagcg cgtcagtaga gcctgcccgc ggttctccaa
     7441 ttcgagcgcc gcggcgaagg aggcgatctc catgttggcc tggagtgccc gtttggaaat
     7501 ccggatgccg ccaggggagt tggcgcagat ctggcccgcc atggtcagcg tttggtcgag
     7561 caggtcatct tcgccggtga cgcggttgag caggcccagg cgttcggcct cctcggcctc
     7621 gaccatgcgc ccggtgaatc cgatctcggc ggctgcggac gggccgatca ggcgcggcag
     7681 tatccatgag gcgcccaggt cacctgcgga gagcccgatc cgcacgaagg cggcgttgaa
     7741 cttcgccgaa ggtgatccga gccggatgtc ggccatcagg gctaacgaca gaccaccacc
     7801 cgcagcgggg ccgttgacag ccgcgatgac tggaacagga agtgcccgca gtgcgctgac
     7861 cgcgcgggcg gcgcgttcct ggcgatccag catgcccagc gccgacaggg atgccagctc
     7921 ctcggcgtcg tctaggtcgt agcctgcgca gaatgccttg ccggcgccgg tgatgatcac
     7981 cacccggata tcgcggtcgt cgccgaccgc gcgcgcaacc gattccagtt cgtcgaacat
     8041 gccaaccgtc atggcgttga gccgttcggg ccggttcagc gtcacctgga ccaaaccatc
     8101 ggcgggcgat tcgagaagca gtgtcttgta acgctgttcg agatcgatca tgaaagtcct
     8161 ccctgctcag acgaattcgg gagcgcgctt gtcgatgaac gcctggaggc cttcgaccgc
     8221 ttcgccccgc tcgaacagct cctgaatctg atcgacctca tatcgcaagc cgtcggccag
     8281 aggtctgtcg tacgcggcat caacggtgcg caccacggcc aattgagccg gcgtcgaagc
     8341 cgcggccaac tcatgggcca actgccacgc cgccgcccgg gcagtgtcgc ccggctcggc
     8401 aagtcgatca accagcccga tccagaaagc ctcatcggcc tcgacctggc gcccggtgag
     8461 catgatgtcc agtgcacggc ttcgcccgac gagacggggc aaccgttggg tacctccgcc
     8521 gccgggaatc aggcccagtt tgacttcagg aagccccagc cgcgccttgg gggccgcaac
     8581 acgaagtgtg caggccatag ccaactcgag accgccgcca agggcaagtc cctcgatggc
     8641 ggcaacagac atcatgggca aggcggcgag gcgttccagc acgccgcgca atgcgtcgcc
     8701 gtagttccgg aacgatgccg cgtcggccga ggacatgtgt ttgatgtccg ctccggcggc
     8761 gaaaaagccg ccaatcgcag aagccagcac gatcaccttg acctcggccg cctctgcgta
     8821 gtcgatcgcg gtgtgcaagc cgtcgatgat ctcgagcccg agcgcgttgg ccggtggcct
     8881 gtcgagcacg aattcgacga tcgccccatg ccggttccaa gtcacgggcg aacgttgttg
     8941 tggttgtgcc acggggtatc tcctttgata gcttggcggg ctggctcagg ccatcgtcag
     9001 tccgccgctg acggacagtg tctggccggt gatgtactcc gcggcatccg atgcgaagaa
     9061 cgcgaccgcg gcggcgatgt ccgacggctg ggccaggcgc ttcatcggca ccgagcgggt
     9121 catcccgccg agcaccttgt cggcgtcctg gccggagctg tcggcgaact tgcgcagcgc
     9181 cggagtgtcg gtggggccgg ggcaaaccgt gttgacggtg acgccctcgg ttgccacttc
     9241 gcgggccagc gtcttggtga acgcgatgat gccgcccttg gcgccggaat agaccgcttc
     9301 gagtgacgag ccgacgcgac cggcatcgga accgatgttg atcacccgtc cgaaaccgcg
     9361 ctccaccatg ccgggcacga cagtgtggat cacgcgaagt gcacccttga aattgatgtc
     9421 aaggatcttg tcccagaagt cttctgaggt tttcaggaac ggcataaagt cgtcccagcc
     9481 ggcgttgttg acgacgactt cgatctcacc gagctgggcg acgacggtct tgaccgcgcc
     9541 gcgcaccgac tcggtgtcgg tgacgtcgac gtggacggcg atggcttcgc cgccggcgtc
     9601 ggtgatgcgc tttgcggttg cttccgcggc ctcgagattg aggtcggcga cggcgaccct
     9661 gaagccgtcg gcacccaggg tcaccgagat gccctctccg atgccttggg caccgccggt
     9721 gacgaatgcg acgcggttgc tcatcgttga atctcctcgc aatgggttgg ttataggtct
     9781 agcggcagga cgtagcgtct gggcccggtt tccactcgat cgggcccgcg acgatggaat
     9841 ggctggtcag cttgcggccg agcacggtct gcgcttcgcg ggcggcgtcg atgatcgccg
     9901 gaagagagac cccggtggag acgcccatct cctcgaacat gctgatcaga tcctcggagg
     9961 cgatgttccc tgtcgagccc gcgggcac
//
LOCUS       DASRJA010000766         5174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4151348,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000766 DASRJA010000000
VERSION     DASRJA010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4151348"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VFC01_34650"
     CDS             <1..820
                     /locus_tag="VFC01_34650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HZN84796.1"
                     /translation="EQAAVAAARAAYIAGFTASSNLEAQRRYAVPALGTSAHAFTLVH
                     TTDTGPDEKAAFKAQVDALGVDTTLLVDTYDITQGVANAVEVAGPELGAVRIDSGDLG
                     VMARQVREQLDRLGATKTRIVVSGDLDEYSIAALRSDPVDIYGVGTSVVTGSGAPTAS
                     MVYKLTEVDGMPVEKRSTHKRSHGGRKQALRTCKSTGTILEEIVYPYGNPPRDESGRD
                     LTVDLVRDGEPVGDLTIDAARRRVKEGLHSLPWDGLKLSKGEPAVPTRMIPARR"
     gene            847..2796
                     /locus_tag="VFC01_34655"
     CDS             847..2796
                     /locus_tag="VFC01_34655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZN84797.1"
                     /translation="MLAKAVASLGGSERSGQIAMAEAVAQAFDTGEHLAVQAGTGTGK
                     SLAYLVPAIARAVDTDEPVVVSTATIALQRQLVDRDLPRLADSLTDALPRRPDFALLK
                     GRGNYLCLNKIHNGSATEPDDRPQEELFEPMAATALGRDVQRLIAWSSSTDTGDRDEL
                     TPGVPDRSWSQVSVSARECIGVARCPFGTDCFAEKARDKAGHADVVVTNHALLAIDAM
                     TDAAVLPEHHLLVVDEAHELVDRVTGVATAELSATSLGVAHRRVTRLIDPELVQRLEA
                     TTATLSSAIHDATPGRIDVLDDEMTTYLTALRDAADRARSAVDTAPSDPKAASARAEA
                     VTALSDISDTATRILSSFVPAITDRTDVVWLDHEDNRGSVRAVLRVAPLSVAGLLRGR
                     LFEHTTAVLTSATLTIGGNFDAMASAWGLAGDEKVNWRGLDVGSPFEHAKSGILYVAA
                     HLPPPGRDGTGSAEQLDEIAGLITAAGGRTLGLFSSMRAAKAAAEIMRERLDTPVLCQ
                     GEDTTSALVQRFADDPETSLFGTLSLWQGVDVPGPSLSLVLIDRIPFPRPDDPLLTAR
                     QRAVAARGGNGFMAVAASHAALLLAQGAGRLLRRVDDRGVVAVLDSRMATARYGGFLR
                     ASLPPFWATTDTIRVRQALERLRGD"
     gene            2818..4263
                     /locus_tag="VFC01_34660"
     CDS             2818..4263
                     /locus_tag="VFC01_34660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HZN84798.1"
                     /translation="MSRRHVVGAVIATCSALLVTACTTTLQGTAVSVFDDPFHVAGLP
                     ATDGPTGLRDDASGPARVVQGTDGGETDELARQAVSDIEEYWGDAYGATFDGQFTPVE
                     ALISWDANGFDATPFCGEDTYGLVNAAFCYDDETIGWDRGELMPALRKSYGDMGMTLV
                     LAHEYGHAVQQQADLKSKNTPTLVAEQQADCFAGAYMRWVAEDKSPRFTLSTGEGLNN
                     VLAAVIAFRDPLLAEGDPEVGVDEHGSAFERLSAFQFGFTDGPSACAAIDMREIGQRR
                     GDLPVLLPEDQTGELPVTEQSVQSIVDAMGILFTPAKPPQLSFKSSDAESCPDARPSP
                     PASYCPATNTIIVDLPELEAMGAQSDSEEGGLASGDNTAYSVLVSRYMQAIQHARGGV
                     LLDNAEAALRTACLTGVATVKMSKNVTTPDGNTITLTAGDVDEAVSGILTNGLAASDV
                     NGESVPSGFSRIDAFRVGVLGDQDRCFKRFP"
     gene            complement(4342..5064)
                     /locus_tag="VFC01_34665"
     CDS             complement(4342..5064)
                     /locus_tag="VFC01_34665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MspA family porin"
                     /protein_id="HZN84799.1"
                     /translation="MLVRPMAVLAACVLICALAAPIASADPETEAVSAEVPAPETAPV
                     DDGRVESSPPKTTNSPDGWTLTVSARDETQRVSAPLTTAISTREYEVGGIYSGLLVGP
                     DEDDDSAQGTLEVGYQIGCGIDMSTSNGVSLTGTVGMNPSIGVVGTDVISPLPEGIVP
                     GIATNVAGGVTVGLKPGLINTIPVSKKEFRGDKPWIMVDGFRIKIDGCVGESFIRSYA
                     FLTRSTEMSDAVAAWYGVTKKV"
BASE COUNT          867 a         1794 c         1710 g          803 t
ORIGIN      
        1 cgagcaggcc gctgtcgctg cggctcgcgc cgcctacatc gcggggttca cggcgtcgtc
       61 aaatctcgag gcgcaacgcc gctacgccgt gcccgcgctg ggcaccagcg cgcacgcgtt
      121 caccctggtg cacaccaccg acaccggccc ggacgagaag gcggcgttca aggctcaggt
      181 cgacgccctc ggcgtcgaca ccacacttct cgtcgacacc tacgacatca cgcaaggagt
      241 cgccaacgcc gtcgaggtgg ccggccccga actcggcgcg gtgcgcatcg actccggcga
      301 cctcggcgtg atggcccgcc aggtccgcga gcaactcgac cgccttggcg ccaccaagac
      361 ccggatcgtg gtgtcgggcg atctcgacga gtactcgatc gccgcgctgc gctccgaccc
      421 cgtcgacatc tacggcgtcg gaacgtcggt ggtcaccggc tcgggcgccc cgaccgccag
      481 catggtctac aagctgaccg aggtcgacgg catgcccgtg gagaaacgca gcacccacaa
      541 gcgatcccac ggcggccgca agcaggcgct gcgcacatgc aaatcgacgg gcacgatcct
      601 ggaggagatc gtgtatccgt acggcaatcc gccacgggac gagtcgggtc gcgacctcac
      661 agtcgatctg gtgcgcgacg gcgaaccagt cggcgacctg accatcgatg cggctcggcg
      721 gcgcgtcaag gaagggctgc acagcctgcc ctgggacggc ctgaaactgt ctaaaggcga
      781 gccggccgtc ccgacccgga tgattccggc acgcaggtag gagtcactac gtcagacgtc
      841 accgaactgc tggccaaagc ggtcgcctcg ctgggcggca gtgagcgaag cggccagatc
      901 gcgatggccg aggccgtcgc tcaggcattc gacaccggtg agcacctcgc cgtgcaggcg
      961 ggcaccggga ccggcaagtc gctggcgtac ctggttcccg cgatcgcgcg ggcggtcgac
     1021 accgacgagc cggtggtggt gtcgactgcg accatcgccc tgcagcgcca gctcgttgac
     1081 cgcgacctac cgcggctggc cgactccctc accgatgcgc taccccgtcg acccgacttc
     1141 gcattgctca agggccgtgg aaattacctg tgcctcaata agattcacaa tggatccgcg
     1201 accgaaccgg acgaccggcc gcaggaggaa ctgttcgaac cgatggccgc gacggcgctg
     1261 ggtcgcgacg tgcagcggct gatcgcgtgg tcgtcgagca ccgacaccgg cgaccgcgac
     1321 gagctgacgc ctggcgtacc ggatcggtcg tggtcccagg tgtcggtttc ggcccgcgag
     1381 tgcatcggcg tggctcgctg cccgttcggg accgactgct tcgcggagaa ggcccgcgat
     1441 aaggcagggc acgccgatgt cgtcgtcacc aaccacgcgt tgcttgccat cgacgcgatg
     1501 accgacgccg ccgtgctgcc cgaacatcac ctgctggtgg tcgacgaggc gcacgagctg
     1561 gtcgaccggg tgacgggcgt ggccaccgcc gaactgtcgg ccacgtcgct cggcgtcgcg
     1621 caccgacgcg tcaccaggct catcgacccc gagctcgtgc agcgcctcga ggccacgacg
     1681 gccacgttgt cgtcggcgat ccacgacgcc accccgggcc gcatcgacgt actcgacgac
     1741 gagatgacca cctacctgac cgcgctgcgc gacgccgccg acagggcccg ctcggccgtc
     1801 gacaccgcac ccagcgaccc aaaggcggcg tcggcgcgcg cagaggcggt gacggcgctg
     1861 agcgacatca gcgacactgc aactcgcatc ctgtcgtcgt tcgtgcccgc catcacggac
     1921 cgcaccgacg ttgtctggct tgaccatgag gacaaccgcg gcagtgtccg cgcggtcctg
     1981 cgggtggcgc cgctgtcggt cgcagggttg ttgcgcggca ggctgtttga gcacacgacg
     2041 gcggtgctca catcggccac gctgacaatc ggtggcaact ttgacgcgat ggcctctgcg
     2101 tggggtttgg ccggcgacga gaaggtcaac tggcgcggcc tcgacgtcgg ttcaccgttc
     2161 gaacacgcga agtcgggcat cctctacgtc gccgcacatc tgcccccgcc aggtcgcgac
     2221 ggcaccggct cggccgagca gctcgacgag atcgccgggt tgatcaccgc ggcaggcggg
     2281 cgcacgctgg gcctgttctc gtcgatgcgc gccgccaagg ccgccgccga gatcatgcgc
     2341 gaacggctgg acacgccggt gctgtgtcag ggcgaggaca cgacgtcggc gttggtgcag
     2401 cggttcgccg acgaccccga gacgtcgctg ttcggcacgc tgtcgctgtg gcagggcgtg
     2461 gacgtccccg gcccgtcgct gtcgctggtg ctgatcgacc gcattccgtt cccccggccg
     2521 gacgacccgc tgttgaccgc gcgacagcgg gcggtggcgg cccgcggcgg caacgggttc
     2581 atggccgtcg ccgccagcca cgcggcgctg ttgttggcgc agggtgccgg gcgcctgctg
     2641 cgacgggtcg acgaccgcgg ggtggtcgcc gtgctcgact cgcggatggc gacggcccgc
     2701 tacggcgggt tcttgcgcgc gtcgctgccg ccgttttggg cgacgacgga cacgatccgc
     2761 gtgcggcaag cactcgaacg tctgcgcggc gattgacttc gtaactatcc tgcctgcatg
     2821 agccgacgcc atgtagtagg tgccgtgatc gcgacctgct cggcgctgct cgtcaccgcc
     2881 tgtaccacaa cccttcaggg cacggcggtt tcggtgttcg acgacccgtt ccatgtcgca
     2941 ggcctgccag ccaccgacgg accgaccggc ttgcgcgacg acgcgtccgg gccggcgcgg
     3001 gtcgtgcagg gcacggacgg cggtgaaacc gacgaactgg cccgccaagc cgtcagcgac
     3061 atcgaggaat actgggggga cgcatacggc gcgacattcg acggccagtt cactccggtg
     3121 gaggcgctga tctcctggga cgcaaacggt ttcgacgcca ccccgttctg tggtgaagac
     3181 acctacggac tcgtcaacgc cgcgttctgc tacgacgacg agaccatcgg ctgggaccgc
     3241 ggcgagctga tgcccgcgct gcgaaagtcg tacggagaca tgggcatgac gttggtgctc
     3301 gcccacgaat acggccacgc cgtgcagcag caagccgacc tgaagtccaa gaacaccccg
     3361 accctggtgg ccgaacagca ggccgactgc ttcgccgggg cctacatgcg ttgggtggcg
     3421 gaggacaagt cgccgcggtt cacgctgtcc accggcgaag ggctgaacaa cgtgctcgcg
     3481 gcggtgatcg cgttccgcga tccgctactg gcggaaggtg accccgaagt cggtgtcgac
     3541 gaacacggtt cggcgttcga acggctgtcg gcgttccaat tcggcttcac cgacggacct
     3601 tcggcgtgtg cggcgatcga tatgcgtgag atcggccagc gccgcggcga cctgcccgtg
     3661 ctgctgccgg aggaccaaac cggcgaactg ccggtcaccg agcagtcggt gcagtcgatc
     3721 gtcgacgcga tgggcatctt gttcacgccg gccaaaccgc cgcagctgag cttcaagtcg
     3781 tccgacgccg agagttgccc ggacgccagg cccagcccgc cggcgtcgta ctgtccggcc
     3841 acaaacacga tcatcgtcga cctgcccgag ctggaagcga tgggcgccca atccgacagc
     3901 gaagagggcg gtttggccag cggcgacaac accgcgtatt cggtgctggt gtcccgctac
     3961 atgcaggcca tccagcatgc gcgcggcggc gtgctgctcg acaacgccga ggccgcgctg
     4021 cgcacggcct gcctcaccgg cgtcgccacc gtcaagatgt cgaagaacgt caccacaccc
     4081 gacggcaata ccatcacgct caccgccggt gacgtcgacg aggcggtgtc gggcatcttg
     4141 accaacggcc tggccgccag cgacgtcaac ggagaatcgg tgccgtccgg cttctccagg
     4201 atcgatgcgt tccgcgtcgg cgtgctcggc gatcaggacc gctgcttcaa gcgcttcccg
     4261 tgattttcat tgcgcgaaca gacgcaaaac tgcccgaaaa gccgcgaaat agggcagttt
     4321 tgtgtctgct cgcgcgggaa gctagacctt ctttgtcacg ccgtaccacg ccgcgaccgc
     4381 gtcggacatc tccgtcgacc gggtcaggaa tgcatacgag cggatgaacg actcaccaac
     4441 gcaaccgtcg atcttgatac ggaatccgtc gaccatgatc cacggcttgt cgcccctgaa
     4501 ctccttcttc gacaccggaa tggtgttgat gagcccgggc ttgagaccga cggtcacacc
     4561 gccggcgacg ttagtggcga tccccggcac gatcccttcc ggcaacggcg agatgacgtc
     4621 ggtgccgacc actccgatcg acgggttcat tcccacggtc ccggtcagcg agacaccgtt
     4681 ggacgtgctc atatcgatgc cgcaaccgat ctgatagccc acttcgaggg ttccctgcgc
     4741 gctgtcgtcg tcctcatcgg ggccgaccag taagccgctg tagatgccgc ccacttcgta
     4801 ttcgcgggtc gaaatcgccg ttgtcagcgg tgcgctgacc cgttgtgttt cgtccctggc
     4861 cgacaccgtg agcgtccagc cgtccggcga gttcgtcgtc ttgggcggcg acgactccac
     4921 ccggccgtcg tcgacgggcg ctgtctcggg cgcaggcacc tcggcagaga cggcctccgt
     4981 ctcggggtcg gcagacgcga tgggcgcggc caaagcgcat atcaacacgc aggcagccag
     5041 cacggccatc ggacgaacca gcacagcgca gccctcctcg gccggaacac attccgccaa
     5101 cgatcattac ctgaccgttc agccgcatac cgcgaaggcg tgccgcatgt cggtgagtct
     5161 ttatccgagc gtga
//
LOCUS       DASRJA010000767        10208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2982312,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000767 DASRJA010000000
VERSION     DASRJA010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10208
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2982312"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFC01_34670"
     CDS             <1..416
                     /locus_tag="VFC01_34670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84800.1"
                     /translation="VEDVLLLGDRHRGARGLKSLRKALPLVDGGAASPRETRLRLLYI
                     DAGLPRPTTQIPIVQGRGRLVRWADMGWEEFMVVSEYDGDQHRTNRWQYTKDIRSLRR
                     ARELGWIVDQVIKEDRPADIVERARTALVRRGWKP"
     gene            complement(413..1129)
                     /locus_tag="VFC01_34675"
     CDS             complement(413..1129)
                     /locus_tag="VFC01_34675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZN84801.1"
                     /translation="MAESTVRIGLVLPDVMGTYGDGGNSVVLRQRLRLRGFDAEIVEI
                     TLSDPVPSELDLYTLGGAEDYAQRLATKHLKQYPGLQQAAGRGAPVLAICAAIQVLGH
                     WYETSSGERVDGVGLLDVTTSPQRERTIGEVVSQPLVEGLTEKLTGFENHRGGTILGS
                     AAQPLAKVISGAGNRAGDGIDGAVQGSVVATYLHGPCLARNPQLADHLLSKVVGELKP
                     LDIDEVAQLRRERLAAPRRV"
     gene            complement(1122..2357)
                     /locus_tag="VFC01_34680"
     CDS             complement(1122..2357)
                     /locus_tag="VFC01_34680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HZN84802.1"
                     /translation="MVTTRGRVALAAGTGARWASRVTGRGAGAMIGGLVALALDKSVL
                     RQLGEGRRTVVVTGTNGKSTTTRMTAAALGTLGSVATNAEGANMDAGLVAALAGSRDA
                     SLAALEVDEMHVPHVADAVDPAVIVLLNLSRDQLDRVGEINHIERTLRGGLARHPSTV
                     VVANCDDVLMTSAAYDSPHVVWVAAGGGWANDSVSCPRSGEIIARDGGHWYSTGTDFK
                     RPTPQWWYDDANIYGPDGLSLPMTLALPGTVNRGNATQAVAAAVTLGADPAAAVAAVS
                     GVDEVAGRYSTVQLGAHSVRMLLAKNPAGWQEALSMVDTDAGSVVISVNGQVPDGEDL
                     SWLWDVNFEHFVEKPVVAAGERGTDLAVRLGYAGVEHTLVHNAVEAITSCPPGHVEVV
                     ANYTAFLQLNRTFGRSGRG"
     gene            2431..3420
                     /locus_tag="VFC01_34685"
     CDS             2431..3420
                     /locus_tag="VFC01_34685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDDh family exonuclease"
                     /protein_id="HZN84803.1"
                     /translation="MSQTWGRPANETGAGWAVVDVETSGFRPGQARIVSIAALALGDD
                     GNVEQSLYTLLNPGVDPGPTHVHGLTAEMLEGQPCFGDIVAPLIDLLKDRTLVAHNVG
                     FDYSFLAAEAELVGAELPIDTVMCTVELARRLELGTENLQLATLAAHWGITQMKPHDA
                     LDDAMVLAQILKPVLVRARERKVWLPVRSVARRQWPSGQVTHDELRPLKMLASRLPCP
                     YMNPGRYVAGRPLVQGMRVALSAEVTRTHEELIERIVHVGLAYTDAVDPETSFVICNE
                     PEPEHGKGYQAHELGVPLVSDDDFMRFLDTVVGGTGIEEFIDTTLGGEQFALF"
     gene            complement(3417..4679)
                     /locus_tag="VFC01_34690"
     CDS             complement(3417..4679)
                     /locus_tag="VFC01_34690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN84804.1"
                     /translation="MGRSDVSADAVVDFDHHSDAFNRDERAINAGLRGRCPVAWNEKY
                     GGFWYVSSYDAVEQVARDGDTFAHRYEPNAADGVNYQGEMGVPRPEGQPPLGIGEVDG
                     PYHLALRRALTPFFSSGAVEKLRPFMEQSVHWFLDQRIADGRMDLVLDYASPVPAILT
                     MRLMGLPYDNWQLYANLFHSVMDASDSDQYNKVIAEVPAMIDGLLKFASTRRAEPADD
                     LTSFLVQFEFDGQRLDDNQLIDILWNLIGGGVDTTTSLTALSLLHLGTQPELRQQLID
                     RPELYRTAADEFLRYCSVNKTLSRTVSRDTVLGGQQLRRNDQVLISWLAANHDERAFE
                     RADEVVLDRAPNRHVAFGLGPHRCIGAPLARVMFEVMVKAVLDRIPDYVVDLEGVHRY
                     SGNPSMTGLGRLPVTFTPGASLGTSRPT"
     gene            4769..5944
                     /locus_tag="VFC01_34695"
     CDS             4769..5944
                     /locus_tag="VFC01_34695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZN84805.1"
                     /translation="MPDLPRMRRERFERLQHQLETQGIDGLVLLGSSAVTYATGAAAP
                     AQDGDRAALFRPVAVVVKGDSAPHLYTLYADGAPTDLPGDHLHGPLFPDLDDGIDAFA
                     AALADHFASGARVGVDHQTHAMLGALPRFDWVDASDVVGAAKLIKTPDEIACIRNAQR
                     LNELAMTDALAILRPGVRQIDLSAVFLRRVFELGASANGIDPIWQVMAPARELGPWTL
                     HGDLAYPTVTTDRFLRQGDVIWVDSGISWEGYASDYGRTWTTGPGPDPRQENQFRRWR
                     TVVDAALDVLKPGVSGLELGAAAIDANDGVRPWIEHFYLAHGVGTESAEMPLIGTDLG
                     EAFDERLIIQPGMVLVLEPVIWEEGFAGYRSEDIVAVTDTGWVKLSGSTYEPYGLTA"
     gene            6097..7257
                     /locus_tag="VFC01_34700"
     CDS             6097..7257
                     /locus_tag="VFC01_34700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZN84806.1"
                     /translation="MTPIARDRGVFVGMMGLEDDARVDFARMRAERRAKVLSGMESHG
                     LDALMLGGVGNVKYVSGARQLGRSGVLPFAPAAVVVRESARVHLLSTWDEGVPPEIGR
                     DELYRLSWNPANLAADLARIPGLREARQVGTDGLTPMFAGLIAELVTSADLVDAGPVM
                     ESARRIKTPEEITCLDVASAIVEAALSAMEEALAPGVTERELLGIYHERIASLGTPMP
                     PSEGVCFATPSSGSVIFRHLASDRPIGDGELVVLAPGALYAGYESGLARTRVAGSPRG
                     DVADVASRCARGLDALIGACRTGSTGADLYRAWKDIGNEDPGVPLAHGLGLGAEPPFI
                     GFGRGGDALLEEGMVLSVQSWVTEEGIGGCLERSTVLIESSGPTALTRYGRL"
     gene            7257..7709
                     /locus_tag="VFC01_34705"
     CDS             7257..7709
                     /locus_tag="VFC01_34705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN84807.1"
                     /translation="MAGHRTVFNHIGLCVSDRERSRRFYEGLLGFEFWWELELPDEGT
                     EKLLQLERPIGVRATYLIRDGLVLELLDYSKRDVIVGPQRVMDHVGLTHMSLSVSDLG
                     EVLAMVEEFGGTLVKETVTEQFAMIRDPDGQLIELLPDSWLSSLPPRP"
     gene            7752..8210
                     /locus_tag="VFC01_34710"
     CDS             7752..8210
                     /locus_tag="VFC01_34710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84808.1"
                     /translation="MATTTDVVQRKATAWDAYRRHGYFFKEAAILTIAIGFCMHLYRV
                     IFGDDVTLQYVVSLESDILLMIPMTYAAVTGILSYRRMVFANRVHKIALTAALVYITA
                     SVPLHLYVAVVLGDVSFYVHMAGYWFSYLLLCLVYPAFLFMLSKVRFRND"
     gene            8203..8652
                     /locus_tag="VFC01_34715"
     CDS             8203..8652
                     /locus_tag="VFC01_34715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84809.1"
                     /translation="MTSAQLVAPTWRDTYRRHGYFFREAAMLTIALGVVIHLLRVIFG
                     DDFAMEYVVTPTTDKILLVPMTYAGITGILLLAHHRVRFANKRHQALFTGSVVYIAGS
                     VPLHIYCSYIVWDTTLMTWFPMWFSYFLLIVVYPAFLTLFAKLQYRN"
     gene            8652..9218
                     /locus_tag="VFC01_34720"
     CDS             8652..9218
                     /locus_tag="VFC01_34720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN84810.1"
                     /translation="MPPNPERRNQILDAAIDILCDDGVGGLTHRQVDSRAGVPAGTTS
                     NYFRTRQALVEATAQRTVDLHWQRVEALQSAIGPLSREALKALMVRMLASDEQFRRWT
                     LARFELFMESTRREELRPLVKELQAAAVKSATLIIEAAGFTPTPERMDVLSRLLNGFV
                     FSNLTMPPEPGALDAADLVDRLLSAFLD"
     gene            complement(9221..>10208)
                     /locus_tag="VFC01_34725"
     CDS             complement(9221..>10208)
                     /locus_tag="VFC01_34725"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_218227.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZN84811.1"
                     /translation="RRDSIILSLHPHNDRGTAVAAAELGYQAGADRIEGCLFGNGERT
                     GNVCIVTLGLNLFSRGIDPQIDFSNIDEIRRTVEYCNQLPVPERHPYGGDLVYTAFSG
                     SHQDAINKGLDAMKVSADEQDADVDDIVWQVPYLPIDPKDVGRTYEAVIRVNSQSGKG
                     GVAYIMKADHGLALPRRLQIEFSQAIQKITDGEGGEVSPKEMWDVFAEEYLAPVRPLE
                     RIRQKVIACETDGGTDTIEATVKIDGVETEIRGAGNGPLAAFVHALSTVGINVQVLDY
                     SEHALTAGEGAQAAAYVEASVNGKTIWGVGIATSITTASLRAVVSAVNRSSR"
BASE COUNT         1727 a         3389 c         3315 g         1777 t
ORIGIN      
        1 ccgtcgagga cgtgctactg ctgggcgatc gccatcgtgg tgcgcgcggt ctcaagtccc
       61 tcaggaaggc gctgccgctt gtcgacgggg gcgccgcctc accgagggag acgcggctgc
      121 ggctgctcta tatcgacgct ggattgccaa ggcccacaac gcagataccc attgtccagg
      181 gtcgcggtcg gctcgttcgg tgggccgaca tgggctggga ggaattcatg gtcgtgtccg
      241 agtatgacgg ggatcagcac cgcaccaacc gttggcagta caccaaggac attcgaagcc
      301 tgcgcagggc aagggagctc ggctggatcg tcgatcaagt aatcaaggag gatcggcctg
      361 ccgacatcgt cgagcgtgcg cggacggcgt tggtacgtcg cggctggaag ccctagaccc
      421 gtcgcggggc ggccaaccgt tcgcggcgca gctgggcgac ctcgtcgatg tccagcggct
      481 tgagctcgcc gacgaccttc gacagcagat gatcggccag ctgcggattg cgggccaagc
      541 acggcccatg caaatacgtt gccaccacac tgccctgcac cgcgccgtcg atgccgtcac
      601 ccgcgcggtt accggcgcca ctgataacct tcgccagcgg ttgcgccgcg gatccgagaa
      661 tcgttccgcc gcggtgattt tcgaagcccg tcagcttctc ggtcaaacct tcgaccaatg
      721 gctgcgacac cacctcgccg atggtccgct cgcgctgcgg cgacgtcgtc acgtccagta
      781 ggccaacccc gtcgacgcgc tcacccgatg acgtctcata ccaatgcccg agcacctgga
      841 tcgccgcaca gatcgccaac accggcgcac cgcggccggc cgcctgttgc aaaccgggat
      901 attgctttaa atgcttggtc gccaaccgtt gcgcgtaatc ctccgctccg cccagcgtgt
      961 acaggtcgag ctccgagggc accggatccg acagcgtgat ctcgacgatc tcggcgtcaa
     1021 atcctcgcag ccgcaaccgt tgccgcagca ccacggaatt gccgccgtca ccgtacgtgc
     1081 ccatcacgtc cggcaacacc agcccgattc gcaccgtcga ctcagccacg tcctgaccgc
     1141 ccgaacgtcc ggttcagttg caggaacgcg gtgtagttgg cgacgacctc gacgtgtccc
     1201 ggcgggcacg aggtgatggc ctcaacggcg ttgtgcacca acgtatgttc gacgcccgcg
     1261 tatcccaggc gtacggccag gtcggtcccg cgctcgcccg ccgccaccac gggtttctcg
     1321 acgaagtgct cgaagttgac gtcccacagc cacgacaggt cctcgccgtc gggcacctga
     1381 ccgttgaccg agatcaccac cgagccggcg tcggtgtcga ccatcgacag tgcctcctgc
     1441 cagcccgccg gattcttcgc cagcagcatg cgcaccgaat gtgcgccgag ttgcactgtg
     1501 ctgtaacggc ctgcgacctc gtcgacaccc gataccgccg ccaccgcggc tgccggatcg
     1561 gcgcccagcg tcaccgccgc ggccaccgcc tgcgtcgcat tgccgcggtt caccgtcccg
     1621 ggcagcgcca acgtcatcgg cagcgaaagc ccgtcgggcc catagatatt cgcgtcgtca
     1681 taccaccact gcggggtggg ccgcttgaag tccgtgcccg tcgagtacca gtgaccgccg
     1741 tcacgcgcaa tgatttcgcc ggaccgcgga cagctcaccg agtcgttggc ccagccgccg
     1801 cccgcagcga cccacaccac gtgcgggctg tcgtaggccg ccgacgtcat cagcacgtcg
     1861 tcgcaattgg ccaccaccac cgtcgacgga tgccgggcca acccgccgcg caacgtccgc
     1921 tcgatgtggt tgatctcgcc gacgcggtcc agctgatcgc gcgacaggtt cagcagcacg
     1981 atgaccgccg ggtccacggc atcggcgaca tgcggcacat gcatctcatc gacttccagc
     2041 gccgccagcg aagcgtcacg cgatcccgcc aacgcggcga ccaaaccggc gtccatgttc
     2101 gcgccttcgg cgttggtcgc gacggatccg agtgtgccca gcgctgccgc ggtcatccgg
     2161 gtcgtcgtcg acttgccgtt ggtcccggtg acgacgaccg tgcggcggcc ctcgccgagc
     2221 tgacggagca cggacttgtc gagcgccaga gccaccaagc cgccgatcat cgcgcctgcg
     2281 ccacgcccgg tgacccgcga cgcccatcgc gcgcctgtcc cggccgcgag cgcgacacgt
     2341 ccacgagtgg tgaccatccc cggcatttta ggtagccgac acgctagcca gcgatccgga
     2401 gatgtcaccg ccgcgtgcaa tcctcaaaac gtgagccaaa cctggggccg cccggcaaac
     2461 gagaccggcg cgggctgggc ggtcgtcgac gtcgagacct ccggcttccg ccccggacag
     2521 gcccgcatcg tgagcatcgc cgccctggcc ctcggcgacg acggcaacgt cgagcaaagc
     2581 ctctacacac tgctgaaccc cggcgtcgac cccggtccca cccacgtgca cggcctgacc
     2641 gcggaaatgc tggaaggcca gccctgcttc ggcgacatcg tcgcgcccct catcgacttg
     2701 ctgaaagacc gcacgctcgt cgcccacaac gtcggcttcg actactcgtt cctggccgcc
     2761 gaggccgagc tcgtcggggc ggagctgccg atcgacaccg tgatgtgcac agtcgagctg
     2821 gcccgccggc tcgaactcgg caccgagaac ctccagctgg cgacgttggc cgcgcattgg
     2881 ggcatcaccc agatgaaacc ccacgacgcg ctggacgacg cgatggtgct cgcccagatc
     2941 ctcaagcccg tgctggtccg tgccagggag cgcaaggtct ggctgccggt acggtccgtg
     3001 gcgcgccggc agtggccgag tggccaggtc acgcatgacg agctgcggcc gttgaagatg
     3061 ctggcctcgc ggctgccgtg cccgtacatg aatcctgggc ggtacgtcgc gggccggccg
     3121 ttggtgcagg gcatgcgggt ggcgctgtcg gccgaggtca ctcgcactca tgaggagctc
     3181 atcgagcgga tcgtgcacgt gggcctggcc tacaccgacg ccgtcgaccc cgaaacgtcc
     3241 ttcgtcatct gcaacgaacc cgaacccgaa cacggcaagg gctatcaggc ccacgagctc
     3301 ggcgtccctc tcgtcagcga cgacgacttc atgcggttcc tcgacaccgt cgtcggcggc
     3361 accggtatcg aggaattcat cgacaccacg ctcggaggcg agcagttcgc gctcttctaa
     3421 gtcgggcgcg acgtccctag ggatgcccct ggtgtgaatg tgaccggcag cctgcccaac
     3481 ccggtcatgc tcggattgcc ggagtatcgg tgcacgccct ccaggtcgac gacatagtca
     3541 gggatccggt cgagcaccgc cttcaccatc acctcgaaca tgactcgcgc gagcggggcg
     3601 ccgatgcagc ggtgtggccc gagcccgaac gcgacgtggc ggttgggcgc ccggtcgagc
     3661 accacttcgt cggctcgctc gaacgcccgc tcgtcgtggt tggccgctag ccaactgatg
     3721 agcacctggt cgttgcgccg aagttgttgt ccgccaagga cggtgtcgcg gctcaccgtg
     3781 cggctcagcg tcttgttgac ggagcagtac cggaggaact cgtcggccgc agtgcggtaa
     3841 agctcgggcc ggtcgatgag ctgctgccgc aactccggct gcgtgccgag gtgcagcaga
     3901 ctcagcgcgg tcagtgacgt agtggtgtcg acgccgccgc cgatcaggtt ccagaggatg
     3961 tcgatgagct ggttgtcgtc cagccgctgc ccgtcgaatt cgaactgaac caggaagctc
     4021 gtcagatcgt cagccggctc ggcccgtcgg gtcgacgcga acttcagcag gccgtcgatc
     4081 atcgcgggga cttcggcgat caccttgttg tactggtcgc tgtcggaggc gtccatcacg
     4141 gagtggaaga ggttggcgta gagctgccag ttgtcgtagg gcaggcccat caaccgcatc
     4201 gtgaggatcg ccggcaccgg actggcgtag tccagcacga ggtccatccg gccgtcggcg
     4261 atccgttggt cgaggaacca gtgcaccgat tgctccatga acggccgcaa cttctcgacg
     4321 gcacccgatg agaagaacgg ggtgagcgcc cgccgcaggg ccagatggta ggggccgtcg
     4381 acctcgccga tgccgagcgg cggctgacct tcggggcgcg gaacacccat ctcgccttgg
     4441 taattcacgc cgtcggcggc gttcggttcg tacctgtgcg cgaacgtgtc gccgtctcgc
     4501 gccacctgtt ccaccgcgtc gtaactggag acgtaccaga agccgccgta cttctcgttc
     4561 cacgcgacgg ggcagcggcc gcgcaggccg gcgttgatcg ctcgctcgtc gcggttgaac
     4621 gcgtcggaat ggtgatcgaa gtcgacgact gcgtcggcac tgacgtcact acggcccatt
     4681 gggctatctt tgcattttta gggagttaag tccgcaggag gtggcaccca tcgatagcgc
     4741 gacaatcggc ggccgggcga ttcccgacat gcccgatctg ccgcggatgc ggcgggaacg
     4801 tttcgagcgt ctacagcatc agctcgagac ccagggcatc gacggcctcg tgctgctcgg
     4861 ctccagtgcg gtgacgtatg ccaccggggc ggccgcgcca gcccaggacg gcgatcgcgc
     4921 ggcgctgttt cgcccggtcg cggttgtagt caagggagat tcggcgccgc acctctacac
     4981 gctctacgcc gacggggcgc caacggacct tcccggcgat catctgcacg gcccgttgtt
     5041 cccggatctc gacgacggca tcgacgcgtt cgcggcggcg ctcgctgacc atttcgcttc
     5101 cggggcccgc gtcggcgtcg accaccagac tcatgcgatg ttgggcgcct tgccgcggtt
     5161 cgattgggtc gatgcctccg atgtggtcgg cgcggcgaag ctcatcaaga cccccgacga
     5221 gatcgcgtgc atccgcaacg cgcagcggct caatgagctg gcgatgaccg atgcgctggc
     5281 gattctgcgg cctggcgttc gccagatcga cctgagcgcg gtgttcctcc gtcgagtctt
     5341 cgaactcggc gcctccgcga acggcatcga tccgatctgg caggtgatgg cgccggcacg
     5401 cgagctcgga ccgtggactc tgcacgggga tctggcctac ccgacggtca ccaccgaccg
     5461 cttcctgcgc cagggtgacg tcatctgggt cgactccggg atcagctggg agggctacgc
     5521 ctccgactac ggccggacct ggaccacggg tcctggtcca gatccgcggc aggagaacca
     5581 gttccggcgt tggcgcacgg tggtcgacgc tgcactcgac gtgctcaagc cgggcgtgtc
     5641 cgggctcgaa ctcggcgctg cggccattga tgcgaacgac ggagtccggc cctggatcga
     5701 gcatttctat ctggcgcatg gcgtcggcac cgagagcgcc gaaatgccgc tcatcggaac
     5761 ggacctgggc gaggccttcg acgagcggct catcatccag ccgggaatgg tgcttgtcct
     5821 cgagccggtc atctgggaag aggggttcgc gggctaccgt tcggaagaca ttgtggccgt
     5881 gacggatacg ggttgggtca aactcagcgg gtcaacctat gaaccgtatg gactgaccgc
     5941 atgagccccg ccgaagacga cgacgagtga ggatcgcagc gaggaacgag cgaggaccgg
     6001 aacgaggagg agtcgagaat gacccccgcc gaagacgacg acgagtgagg atcgcagcga
     6061 ggaacgagcg aggaccggaa cgaggaggag tcgagaatga cccccatagc tcgtgaccgc
     6121 ggggtgttcg tcggcatgat gggcctcgag gacgacgcgc gtgtcgactt cgcccgcatg
     6181 cgtgccgaac gtcgcgcaaa agtgctctcc ggcatggaaa gtcacggcct cgatgcgttg
     6241 atgctcggag gcgtcggcaa cgtgaagtac gtgtcggggg cgcggcagct cgggcgctcc
     6301 ggagtgctgc ctttcgctcc ggcggcagtg gtggtgaggg aatccgctcg cgtgcatctg
     6361 ctttcgacgt gggacgaggg agtgccgccg gagatcggcc gcgacgagct gtaccgcctg
     6421 agctggaacc cggccaacct ggctgcggat ttggcgagaa tccccggctt gcgtgaggcg
     6481 cgacaggtgg gcaccgacgg gttgacaccg atgttcgccg ggttgatcgc tgagctcgtc
     6541 acgtcggcag acctcgttga cgccggtccc gtcatggaga gtgcccgacg gatcaagacg
     6601 cccgaggaga tcacctgcct ggacgtcgcg tcggcgatcg tggaagcggc gttgagcgcg
     6661 atggaagagg cgttggcgcc gggcgtcacc gagcgcgaac tgctcggcat ctaccacgag
     6721 cggatcgcaa gcctcggcac accaatgcca ccgtcggaag gcgtctgctt cgcaacgcca
     6781 tctagcggct cagtcatttt ccggcactta gcatccgacc ggcccatcgg cgacggcgag
     6841 ttggtggtgc tcgcgcctgg cgcgttatac gccggctacg agtctggcct ggcccgtacc
     6901 cgggtcgccg gcagtccacg cggggacgta gcagatgtgg cgtcgcgatg cgcgcgcgga
     6961 ctggacgcgc tgatcggcgc atgccgcacc ggcagcaccg gcgcagacct gtaccgggcg
     7021 tggaaggaca tcggcaacga agatcccggc gtgccactgg cgcacggtct cggactgggc
     7081 gcagaaccgc ccttcatcgg cttcggacgc gggggagacg ccttactcga ggagggcatg
     7141 gtgctgagcg tgcagtcctg ggtcaccgag gaaggcatcg gcggatgcct ggaacgcagt
     7201 acggtgctca tcgaaagcag cggaccaacc gcccttaccc gttacggaag gttgtaatgg
     7261 caggacaccg aacggttttc aaccacatcg gtctatgtgt aagtgaccgt gagcggtcgc
     7321 gccgcttcta cgaaggactg ctcggcttcg agttctggtg ggaactcgaa ctgcccgacg
     7381 agggcaccga gaagctgctg cagctcgaga gaccgatcgg agtgcgtgcg acgtacctga
     7441 ttcgcgacgg cctcgtgctg gaactcctcg actattcgaa gcgcgacgtc atcgtcggcc
     7501 cgcagcgggt catggaccac gttggactga ctcacatgtc gctgtcggtg tccgatctcg
     7561 gcgaagtgct ggcgatggtc gaagagttcg gcggcactct ggtcaaggag accgtcaccg
     7621 agcaattcgc gatgatccgt gaccccgacg ggcagctgat cgagctgctc ccggactcgt
     7681 ggttgtcctc gttgccacct cggccatagc gcctattgca atcactacgc ccgtagtgat
     7741 accttcaacc catggcaacc actacagacg tagtacaacg caaggccaca gcttgggatg
     7801 cctaccgccg ccacggctac ttcttcaagg aagccgcgat cctcacgatc gcgatcggct
     7861 tctgcatgca cctttaccgg gtgatattcg gtgacgacgt cacgctgcaa tatgtcgtct
     7921 ccctagagtc ggacatcctg ctgatgatcc cgatgacgta tgcggcggtg accggcatct
     7981 tgagctaccg caggatggtg ttcgccaacc gcgtgcacaa gatcgcgctc accgcggcgc
     8041 tggtgtacat caccgcgagt gtgccgctgc acctgtacgt ggcggtcgtg ctgggtgacg
     8101 tcagcttcta tgtgcacatg gccggctatt ggttcagcta tctgctgctc tgcctggtct
     8161 acccggcatt cctgttcatg ctgtcgaaag tgaggttccg caatgactag tgcacaactc
     8221 gtcgcaccga cgtggcgaga cacctatcgc cggcacggct acttcttcag ggaagccgcg
     8281 atgctgacca tcgcccttgg tgtcgtcatc cacctgctga gggtgatctt cggcgacgac
     8341 ttcgcgatgg agtacgtcgt gacgccgaca accgacaaga tcctgcttgt gccaatgact
     8401 tacgcgggca tcaccgggat cctgttgttg gcgcatcacc gcgtgcgctt cgcgaacaag
     8461 cgacaccagg ccctgttcac cggctcggtg gtctacatcg caggtagcgt gcccctgcac
     8521 atctactgtt cctacatcgt ctgggacacc accctgatga cgtggtttcc gatgtggttc
     8581 agctacttcc tgctgatcgt ggtgtacccg gcgttcctga cactgttcgc caaactgcaa
     8641 tataggaact gatgcccccc aatcctgaac gacggaacca gatcctcgac gccgcgatcg
     8701 acatcctgtg cgatgacggc gtcgggggtc tgacgcatcg gcaggtcgac agccgcgccg
     8761 gcgtgcccgc gggcaccacg tcgaactact tccggacgcg gcaggcgctt gttgaggcga
     8821 ccgcccaacg gacggtcgat ctgcattggc aacgcgtcga ggcgctgcag tcggcgatcg
     8881 gcccgctgtc gcgagaggcc ttgaaggcgt tgatggttcg catgctcgca tccgacgagc
     8941 agtttcgccg ctggacgctg gcgcggttcg agctgttcat ggagagcacc cggcgcgaag
     9001 aactgcggcc gttggtgaag gagcttcagg ctgccgcggt gaagtcggcg acgctcatca
     9061 tcgaggccgc cggcttcacc ccgacaccgg agaggatgga tgtactcagc aggctgctca
     9121 acggctttgt gttcagcaac ctcaccatgc cgcccgagcc gggcgcgcta gatgccgccg
     9181 atcttgtgga ccgactgctg agtgccttcc tcgactagcg ctagcgtgag gaccggttga
     9241 ccgccgacac cacagcgcga agcgatgcgg tggtgatcga cgtcgcgatg ccgacgcccc
     9301 agatggtctt gccgttcacc gacgcctcca cgtatgccgc ggcctgcgcg ccttcgccgg
     9361 ccgtcaacgc gtgctcggag tagtccagca cctgcacgtt gatgccgacc gtcgacaacg
     9421 catggacgaa cgccgcgagc ggaccgttgc cggcgccgcg gatctccgtc tcgacaccgt
     9481 cgatcttgac cgtcgcctcg atcgtgtctg tgccaccgtc ggtttcgcag gcaatgacct
     9541 tctgacggat gcgctccagc ggccggacgg gcgccaggta ctcctcggcg aagacgtccc
     9601 acatctcctt cggcgagacc tcgccgcctt cgccgtcggt gatcttctga atcgcctggg
     9661 agaactcgat ctgcaaccgg cgcggcagag ccaggccgtg gtcggccttc atgatgtagg
     9721 ccacgccgcc cttgccggac tgcgagttga cccggatgac cgcctcgtag gttcggccga
     9781 cgtccttcgg gtcgatcggc agatacggca cctgccacac tatgtcgtcg acatcggcat
     9841 cctgttcgtc cgccgacacc ttcatcgcgt ccaggccctt gttgatggcg tcctggtggc
     9901 tgccggagaa cgcggtgtac accaggtcgc cgccgtaggg gtgccgttcg ggtaccggca
     9961 actggttgca gtattcgacg gtgcgacgga tctcatcgat gttggagaag tcgatctgcg
    10021 ggtcgatgcc gcgggagaac aggttcagtc ccagcgtgac gatgcagacg ttaccggtgc
    10081 gctcgccgtt tccgaacagg cagccctcga tccggtcggc gccggcctga taccccaatt
    10141 ccgctgcggc gacggcggtt ccgcggtcgt tgtgcgggtg caggctcagg atgatgctgt
    10201 cccgacgg
//
LOCUS       DASRJA010000768        27529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1102836,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000768 DASRJA010000000
VERSION     DASRJA010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 27529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27529
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102836"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1200)
                     /locus_tag="VFC01_34730"
     CDS             complement(94..1200)
                     /locus_tag="VFC01_34730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN84812.1"
                     /translation="MDFNLSKEQELLRDGLAKFLSTRYDLEKSRAAAKTGTGWQPDIW
                     RGFADELGILGAALPEEVGGIGGGPVEVMVIAEALGHALAVEPYVDTVVVAGGLLRRA
                     GGTLANELLEKIVAGTAVVALAATEPGSGDDWQAVSTVAVRAGDEWLLRGSKVVAMSA
                     PVATHLLVTARTPQGLSLFLVDADWSAIDGHHYRTVDDRRASDLVLDGVRGVLLGEEG
                     QASPSLAQARDEGAAAICAEAVGCMRKVLADTVEYCKQRQQFGQPIGSFQVLQHRMVD
                     MYMEVEQAVAAVYLAVLNLEASPDVRARAVSAAKATIGRAARFVGQQAVQLHGGMGMT
                     EELAIGHYFKRLTALQYEFGSTDYHVARYASLTK"
     gene            complement(1201..2433)
                     /locus_tag="VFC01_34735"
     CDS             complement(1201..2433)
                     /locus_tag="VFC01_34735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN84813.1"
                     /translation="MDLAWSATDSAFRDEVRAFLAEKLTPGLRRAGRLMTSVYADHEA
                     SMEWQRILHERGWAAPAWPVEYGGCDWSLTQHYIFSRESQLAGAPSLSPMGIRMVAHA
                     IIKFGTDAQKEYFLPRILTGEVFFCQGYSEPEAGSDLAALSMAAIDDGDDLVCTGSKI
                     WTTHAREANWMFALVRTSKTQKKQQGITFVLIDMTSPGLQIRPLVMTSGEEIQNQVFF
                     DEVRVPKSNVLGQIDDGWTVAKYLLEFERGGGAASPALQVMAEEIATAAANQPAPGGG
                     RLLDDAAFARKLADARIRTEVLEILEYRVLAALAGGGHPGPGASMLKVLATELSQSLT
                     ELAMEAAGPRGRVYQPHATCPGGPVSEFEPPPDGYVSGEAWQAVAPLRYFNDRAGSIY
                     AGSNEIQRNILAKAALGL"
     gene            2622..2984
                     /locus_tag="VFC01_34740"
     CDS             2622..2984
                     /locus_tag="VFC01_34740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZN84814.1"
                     /translation="MTLLKELPALVVLERFPVPVLAIAEDGTILFANEAFGQMLGYPA
                     GVVRALKFRQIFHTLPTDESAVSVVRAHADLIVELLHQDGSTVRARMSKSALLRGDDP
                     VALATFQDLTERLWVDEL"
     gene            complement(2985..3644)
                     /locus_tag="VFC01_34745"
     CDS             complement(2985..3644)
                     /locus_tag="VFC01_34745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="HZN84815.1"
                     /translation="MVRQARSEATRRKIITSAVELFNEIGYPATGLGDIIDRAEMTKG
                     ALYYHFDSKESLATAIIEEASAHLVAAFANITASSAPALENIIHGVFVVADLLSTDKI
                     ARSGTQLLRAFGEFNDAAALVYGGWVAEMTERTRQAIDEGDLRAELDPQAVAETIVGS
                     MLGAELLANATTAGADVLARVARTWKVLLPAIVNQESLSYFDEFLARESLRHSDTPLT
                     D"
     gene            complement(3719..4381)
                     /locus_tag="VFC01_34750"
     CDS             complement(3719..4381)
                     /locus_tag="VFC01_34750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN84816.1"
                     /translation="MTEQLDRRADTTRLQILRAASHQFARKSYSLVNLDDILADAEVT
                     KGAMYFHFRSKYALASAIIEHRGTVARASVDQLLAQKLSGLETLIDISYLVAVDDIGD
                     ETARAGLNLLESIGRTDGLQEHIHGQWVTAFAAIARKGIKDGDVIGDRDPQGIARLLV
                     SMYLGLRQTSNLAEPERFLHDLEDLWVLMLRGFVNPDRIDYLEKFIKRRTMVALKRAT
                     PL"
     gene            4948..5883
                     /locus_tag="VFC01_34755"
     CDS             4948..5883
                     /locus_tag="VFC01_34755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZN84817.1"
                     /translation="MNWDEEVDVVSTGSGVAGLAHALAVVNMGGEVFVADSCSDVDPG
                     RTSVAVRSRVDRLHWLEVDIADPDTNEYFAALSSDLGPLTRSAGDVDVPIRMVDHEEP
                     VDARGPVAPFIGARLRDWAARCLVSPYGYLYTRVSDWRSTTLRTLDGESLQVAEIGSI
                     TPDPANVGGSVLDWLSTQARDRCIEINRSTSLQRIVFEEGHVVGAVFTTPDGPLAVRA
                     RHGVTVASGGSQAALAGEQPLPLDGGSLRVCLVGQTASRFGRVELLTTEPFARDAASN
                     CRPANRQLHANLHATHSPLQTWRCGKVNGHSSFGQ"
     gene            complement(5830..7629)
                     /locus_tag="VFC01_34760"
     CDS             complement(5830..7629)
                     /locus_tag="VFC01_34760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN84818.1"
                     /translation="MPSSLELVVTSVATQLMGVSAATSVAVSERVLADLVEYFDVDGG
                     FLRQNDHGIHASILVAEWPVRPAISDPDPLAVIYFADADPVFALSEYQKEPVVFRTEA
                     VTSMACVPLLSGDVTTGVIGFVEAGDRVWTSEELNALKAIASLFAQVQARVLAEEQLR
                     YLADHDDLTGLHNRRALLEHLDARLAAGQPGPVSALFFDLDRLKAINDYLGHTAGDWF
                     IRVLAERLREGAEGPNLIARLGGDEFVVVPTTPMDADTAEALAYRLQSTLCERVAIDG
                     EVLTRTVSIGVALGVPGRDTTSDLLRRADQAVLTAKNSGGNTVAVFSDDMSMENEFRN
                     DIELHLQSVIENGSLLLHYLPEVDMRTGEILAAEALVRWEHPTRGLLSPDSFIGVAES
                     INLAGELGRWVMRAACSEFAGWRSRGLGHDTVLRINVSPVQLVTDGFVESVAGIISEF
                     GLDGGSVCLEITENVVVQDIETTRITLAGLKEAGVQVAIDDFGTGYSVLSHLKSLPVD
                     TLKIDKGFVRDLGSNPGDLAIVRAIIALAEAFGLHLVAEGVETETAALTLLRHGCYRA
                     QGFLLSRPIAGGAMAALLAKGRVPVDFSAAPRL"
     gene            complement(7778..8644)
                     /locus_tag="VFC01_34765"
     CDS             complement(7778..8644)
                     /locus_tag="VFC01_34765"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN84819.1"
                     /translation="MNGFRPLWRIPGIVARERRHPAGRERTPEPMVMDDPESVAQFHA
                     AGASNPGIQAVYDLGSRALDALLPTGARLLDLGVGSGRALAAVLRRRPDLEITAVDLA
                     PNMLATAQDLLRTEGFDGRVELVHADITALPDRLARGAWNGVSCMWTLHQLPDFDGLR
                     AALRQIADVRRHSGAAIWISDFQRLRDPSTASAMLRCVDPSIRSVAYKDALASEAAAF
                     THAELSSELAAAGLGGVQYGFARPLPYLQAYWMRAAGAKASASLGRHTPLPGPARRDA
                     ALLRWGFTAKPF"
     gene            8769..10154
                     /locus_tag="VFC01_34770"
     CDS             8769..10154
                     /locus_tag="VFC01_34770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN84820.1"
                     /translation="MVELRLESTLAELPLAPKNPLPYRQRRRAAKSFHTGMETLRDAG
                     GPITRFTLGPRLLAPPLVLATSPGAIRDILSIKDDSVDKTTSVFVEMRRVLGANLSNL
                     PYEPWLSRRRTLQPVFTKQRVREFGGHMAQAAESVCDEWPDGGVVDLDAESRKLTLRA
                     LGRSVLGIDLDHRADEVDQPLRVALKHAMHRAILPVRVPSWLPIPGARRARAASTKVH
                     RLAQEILRSCRADPTRVAPLVHALIDAKDPVTGESLSDEEICEELIIFLFAGHDTTAT
                     TLAYALWALGRNPECQDRVAAEVAELPDGPLTVDDMPRLNYTVQVLRESLRLCPPGPT
                     GTRMACRDIEVGGYRVPAGTMLVVGRMAVQRDPGLWDAPLKFDPDRFSPDNMKKLDRW
                     QYIPFGGGPRSCIGDHFAMLETTLALATVIRRAEIDSVEEEFPLAVHFTMIAGAPIPA
                     RVRPRRPGVSA"
     gene            10151..12286
                     /locus_tag="VFC01_34775"
     CDS             10151..12286
                     /locus_tag="VFC01_34775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv1355c family protein"
                     /protein_id="HZN84821.1"
                     /translation="MIATDEAGVFSAQLLNPADADDSVLLDRLRQDSRIEFTDHRAEL
                     LKCLRLLCPSPDADILSEPTSWAYYPWRRAVVGVLGPRAYRALRLDRNRHLITVAEQD
                     RLNGLRIGVAGLSVGHVIAHTLAAQGLCGTVRLADFDDLELTNLNRVPATALDIGVNK
                     AVVAARRIAELDPYIHLDVMTAGVTPESLSDFLEDIDIVVEECDSLDMKVMIREAARE
                     RRLPVLMATSDRGLVDVERYDLEPDRPILHGLLGQVDASLLASLPSRDKLPYMLSHIN
                     AAQTSPRLIASLVEVTNTLSTWPQLAAEVTLGAAAVAEAVRRIGLQQPLRSGQTRIDI
                     SQALDDVAEPPARIDVQRLHEEPADDEQLDGTPTEPVAAVAQAAMRAPSGGNAQPWHV
                     EAQANSVVIRLAPECTSMMDVGFRGSAVAIGAALFNARVAAAAWNVLGPTVWKTDDVT
                     CPLEVALRLDHGGDAELADLYQPMLRRETNRHLGVSEPIGAETVALLHDVARREGARL
                     CLLQAREDFEKVASIFAAADRIRYLTPHLHKEMISELRWPGDEPADTGIDVRSLELDP
                     AELAVLDVLRRADVMAELAQWNAGEALGDEVRKRIRASSAVAVITVHGHELIDFACGG
                     SAVEAVWVVAQRLGLAVQPISPVFLHAVYRDEIVELSPSFATELQNLHADFRQLAGTR
                     NDESQVLVLRFAHARGASVRSKRRGLILK"
     gene            12534..14405
                     /locus_tag="VFC01_34780"
     CDS             12534..14405
                     /locus_tag="VFC01_34780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZN84822.1"
                     /translation="MPRSLEVVVTEVATRLIGATAATQPRVSQEVMAELVDVLGVDAS
                     FLRHNDHAIRATKLIAEWPVRPDIPDPDPLGTVFFADADPIFAAAEHAKEPLILRPDP
                     ASDDYQRRVEEGRGVPAISMASVPLISGDVTTGALGFVKFGDKSWRPEEINALKAIAS
                     LFAQLQARVVAEEQLRFLADHDDVTGLHNRRSLLIHLDERLSPGRPGPVATLFIDLDR
                     LKAINDYLGHTAGDQFIRVMSERLRAGTGGQHLIARLGGDEYVVVPTGPTDAEAAEAL
                     ADKLHSMLVERVMLGSEMLARTVSIGVAVGTPGLDTTSDLLRKADQAVLTAKNSGGNR
                     VSVFSDDMSLTSELRNDIELHLRGVIESGALVLHYLPEVDMRTGQVLAAEALVRWQHP
                     TRGLLMPDVFLSVAESINFAGELGRWVMRNAFAEFSRWRSQRVGQDALLRLNISPIQL
                     VGSEFVEHVAGVLDEFGLDAGSVCLEVTESVVVEDIQTARVTLARLKEIGVQIAIDNF
                     GTGYSVLSQLKSLPVDMLKIDKSFIRELGSNPGDMAIVKAIVALANAFELELVAAGVE
                     TQVAAEALLELGCYRAQGYLLSRPLTSEAMKLLLTEGHLQIPPVKPRGKFLLLGKDH"
     gene            complement(14328..15071)
                     /locus_tag="VFC01_34785"
     CDS             complement(14328..15071)
                     /locus_tag="VFC01_34785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84823.1"
                     /translation="MTVPQTEPASRIVVSLKNTPNYWDRDSECRIVFAQPAVERELWA
                     DYVRGACQSYRKHGVEKALDMDALRSGADTILFVACVDGAGRVVGGVRGRGPYRSTDE
                     CHAILEWNGQPGQDAVRKMVSDRLPFGVVEMKTAWVTDDSERNGLLTTAMARTSSHTM
                     NLLDIQFIMATAAEHVLKQWLSSGGLLATKIPATPYPDHRYRTKLAWWDRSTFAKHAD
                     PKQLSMIFAEQKKLAPRLDRRYLQMAFGE"
     gene            15547..16446
                     /gene="purU"
                     /locus_tag="VFC01_34790"
     CDS             15547..16446
                     /gene="purU"
                     /locus_tag="VFC01_34790"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HZN84824.1"
                     /translation="MKQVDEGYATAGRGPVKDVGRLLLRCADRPGLVAAVSAFLADAG
                     ANIVSLDQHSTEQSGGTFMQRTIFHLPGLTAARDALERDFREQVASQFDMDFRLTEAA
                     KPKRVAIMASKEDHCLLDLLWRNRRGELDLSVVMVISNHPDLADQVRPFSVPFLHVPA
                     TKDIREEAERRQLDLLRGNVDLVVLARYMQILTPQFLAEVGCPLINIHHSFLPAFIGA
                     APYRRAKERGVKLVGATAHYVTEDLDEGPIIEQDVVRVDHRHGVDDLARLGADVERLV
                     LSRAVLWHCEDRIIRYGNQTVVL"
     gene            16467..16919
                     /locus_tag="VFC01_34795"
     CDS             16467..16919
                     /locus_tag="VFC01_34795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZN84825.1"
                     /translation="MKKFSGLDEFVAAQGSQLGPTDWLEVTQDRVNLFADATDDHQWI
                     HVDPERAANGPFGGTIAHGLLTLSLLPHFTHQLYTVDNIAMAINYGYNKVRFITPVRV
                     GSKIRARAEIAKVDQLEGAVQATVTVTVEIDGSDKPAAVAESIVRFIG"
     gene            complement(16925..17746)
                     /locus_tag="VFC01_34800"
     CDS             complement(16925..17746)
                     /locus_tag="VFC01_34800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZN84826.1"
                     /translation="MSGRLEGKVAFITGAARGQGRAHAVRMAAEGADIIAIDIAGKLP
                     PCVPYDSATPDDLAETVNLVEATGRKILASVVDVRDVAGLNEAVDDGVAALGRLDIIV
                     ANAGVSAPQTWDAITPEDFRDVMDINVTGAWNTVMAGAQKIIDGGRGGSIILISSAAG
                     IKMQPFMVHYTASKHAVTGMARAFAAELGRHSIRVNSVHPGAVNTLMGSGDMIGSLGR
                     AIETYPQLAQMITPFLPAWAAEPDDIADAVCWLASEESKNVTAAAISIDQGMTQY"
     gene            complement(17743..18909)
                     /locus_tag="VFC01_34805"
     CDS             complement(17743..18909)
                     /locus_tag="VFC01_34805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN84827.1"
                     /translation="MTTEVSDDDFQEILAQTRHFVRSAVVPREQEILAEDRVPDDLRE
                     QAEKMGLFGYAIPQEWGGLGLNLAQDVELAMELGYTSLALRSMFGTNNGIAGQVLVGF
                     GTDEQKGRWLEPMASGLVASFALTEPGAGSNPSGLRTKAVRDGGDWIIKGQKRFITNA
                     PMADLFIVFARTRPADDDGPGIAVFLVPADSSGVVVGAKDAKMGQEGAWTADVSFDDV
                     RVPDEALIGGSEDIGYRAAMTSLARGRVHIAALAVGIAQRALDESVAYAATATQGGTP
                     IGNFQLVQAMLADQQTGVLAGRALVRDTARLWVSGEDRRIAPSAAKLYCTEMAGKVAD
                     LAVQVHGGSGYMREVPVERIYRDVRLLRLYEGTSEIQRLIIGSNLVKAAQKEQK"
     gene            19021..19761
                     /gene="fabG"
                     /locus_tag="VFC01_34810"
     CDS             19021..19761
                     /gene="fabG"
                     /locus_tag="VFC01_34810"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZN84828.1"
                     /translation="MSLLTGQTAVVTGGAQGLGLAIAERFISEGARVVLGDLDLGATE
                     AAAKQLGGDDVALAVRCDVTLADEVDALVTAAVERFGGLDIMVNNAGITRDATMRKMT
                     EEQFDQVIAVHLKGTWNGTKAAAAIMRENKRGAIVNMSSISGKVGLVGQTNYSAAKAG
                     IVGLTKAAAKELAHLGVRVNAIQPGLIRSAMTEAMPQHIWDQKLAEVPMGRAGEPAEV
                     AKVALFLASDLSSYMTGTVLEVTGGRHI"
     gene            19761..20975
                     /locus_tag="VFC01_34815"
     CDS             19761..20975
                     /locus_tag="VFC01_34815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZN84829.1"
                     /translation="MRDAVICEPVRTPIGRYGGMFKSLTAVDLGVAALKGLLERTGIE
                     PAAVEDVILGHCYPSSEAPAIGRVVALDAGLPVTVPGMQIDRRCGSGLQAVIQAALQV
                     ANGDNDVVVAGGAESMSNVAFYSTDMRWGGARGGVKVHDGLARGRTTAGGRHYPVPGG
                     MLETAENLRRQYNIPRQEQDELAVISHQRAVAAQKDGILAEEIIPVAVRTRQGEELID
                     IDEHPRADTSVETLSNLKPVLLCDDPQATVTAGNSSGQNDAASMCIVTTPQKADELGL
                     TPLVRLVSWGSAGVAPNIMGIGPVPATEVALGKASLKLSDIDLIELNEAFAAQALAVM
                     REWKFGAADLDRTNVHGSGISLGHPVGATGGRMLATLARELNRRGARYGLETMCIGGG
                     QGLAAVFERVGA"
     gene            20972..22165
                     /locus_tag="VFC01_34820"
     CDS             20972..22165
                     /locus_tag="VFC01_34820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN84830.1"
                     /translation="MTRLAQTLGLTEFQTEIITTVRQFVDKEVIPTAQELEHSDTYPQ
                     AIVDAMKDMGLFGLMIPEEYGGLGESLLTYALCVEELARGWMSISGVINTHFIVAYMI
                     RQHGTDRQKQHYLPRMATGETRGAFSMSEPELGSDVAAIRTRATRNADGTYTIDGQKM
                     WLTNGGSSTLVAALVRTDEGADKPHRNLTAFLIEKPTGFGEVAPGLTIPGKIDKLGYK
                     GIDTTELIFDGFVASADDILGEAPGQGFFQMMDGIEVGRVNVSARACGVGIRAFELAV
                     RYAQQRHTFGKPIADHQAIAFQLAEMATKIEAAHLMMVNAARLKDSGERNDVAAGMAK
                     YLASEFCAEVTQQSFRIHGGYGYSKEYEIERLMRDAPFLLIGEGTSEIQKNIISKRVL
                     ADYRI"
     gene            22169..22837
                     /locus_tag="VFC01_34825"
     CDS             22169..22837
                     /locus_tag="VFC01_34825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN84831.1"
                     /translation="MTTPDFAFRPQLAEDVARYVRKRIFDGTYAAGQYIRLDTLAAEL
                     GVSVTPVREALFVLSAEGLLSQQPRRGFVVVSVTGRDLTDVANVQAHIGGELAARAAV
                     NITDQQLRELEKIQTELEDAYASDDDDRAVRLNHEFHKAINVAADSPKLAQLMSQITR
                     YAPESVFPTITGWPEQSTDHHRKLLAALEKHDEGLARMAMSEHLAAGAAPLIEHLTQR
                     GVVD"
     gene            complement(22889..24433)
                     /locus_tag="VFC01_34830"
     CDS             complement(22889..24433)
                     /locus_tag="VFC01_34830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZN84832.1"
                     /translation="MPDTIDWLVRTRAQQLNDKPMVIDTDSRISYAELEGSTRALAAA
                     FIEAGVAKGTRVGLIMPNRVQWVQLAMALTRIGAVLVPLSTLLQPPELIAQLRAASVQ
                     ALITVEEFRGHRYLDDLADVVNTPELPALRTVWMPEHVAGSHGGQMVDEMAAAVTPSD
                     ALVIMFTSGSSGPPKGVIHSHGNALAAVRSGLAARCIDADTRLYLPMPFFWVGGFGSG
                     ILSALLAGATLVTEEIPRPETTLRLLERDRVTLFRGWPDQAEALARQSASVGADLSAL
                     RPGSLEALLPPEQRAEPGARAKLFGMTESFGPYCGYPADTDMPRTAWGSCGKPFDGME
                     VRIVDPDTGHPVPAGTIGMIQLRGPHTLRGICRRSREDLFTADGYYPTGDLGHLDDDG
                     FMFYHGRFDDMFKVSGATVYPSEVEQALRSIDGVENAFITDVSGAVGAVVISGRTVDD
                     LRAEARKRLSSFKVPAVWLTVKSDDDIPRGTTGKVDVRRLRALLRELGALPSQAGGPP
                     RVQGGT"
     gene            complement(24426..25910)
                     /locus_tag="VFC01_34835"
     CDS             complement(24426..25910)
                     /locus_tag="VFC01_34835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZN84833.1"
                     /translation="MPTETLTVGEVLRRQARAGGEHTLLVCDAERLSYAEADFRSAQL
                     ARGLIDLGAGKGTHVGVLFPNGAAWIIAMLAAARIGAVVVPFSTFATVPELATQLADS
                     DTEILLATASFRSHDYRQRLAGIKEAAPLLRHVLIDSAPGNTADATLLEAMEHDVDES
                     DPLAIVYTSGSTSTPKGVVHTHASLLGHQKALNEIRGLTADDKLFCNSPFFWIGGIAF
                     AVLATLLAGSTLVCSNATDAGETLDLLESEKPTMTNGFVAGIAQLAHHPSLPQRDLSS
                     LRRGNLYPIMAPEVRPADPELRHTMLGMTEAGSVITISDDESDQPEHRRGSFGRPAPG
                     FETRILDGELCIRGPFVMQRYYKRSREECFDADGWFHTGDLVRTDADGFVYFLGRRGA
                     MIKTAGANVAPAEVERAIAKVTGGTVAHVLGLPDPERGQVVAAIVALEDGADFDEAAV
                     RERLKAELSVYKIPKRFAAVPRSEIPVLSSGKIDLTALQKVFDA"
     gene            complement(25900..27318)
                     /locus_tag="VFC01_34840"
     CDS             complement(25900..27318)
                     /locus_tag="VFC01_34840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZN84834.1"
                     /translation="MHPLSRRITDVLELQPDAAAIEYNGQWSTWRQVAELANRIAEVG
                     ETEVGMLLRNTPTHVAALLGVLLGGGTVVTINPSRGDDRTRADIAALDLPVVVGEPDD
                     LASMVSAGPTAIPISGLDAAPAMPARGAGRPGVAVRMLTSGTTGTPKRIDLTYDMLAR
                     SVITSPPPTELRRGVAIVNAPLVHIGGVFRMLQCVCEARPFALLNRFELNQWADAVRR
                     HRPRAVSLVPAALRMVLHSDLTRDDLASIRAVTSGTAPLSAEDADAFTAKFGIPVLTS
                     YAATEFGGGVAGWTLSDYQKHWHDKRGSVGKASLGAQLRVVDDDGTPLGPGQAGLLEV
                     KPGQLGPSADWMRTTDIARIDGDGFLWILGRADQAIIRGGFKVMPDDVRTALESHPAV
                     RGAAVIGRPDDRLGEAPVAMVELRDTTDAGALIEFLRGRLARYEIPTDIAIVDQIPRT
                     PSGKADLGAVREFFSHPAEHAN"
     gene            complement(27318..>27529)
                     /locus_tag="VFC01_34845"
     CDS             complement(27318..>27529)
                     /locus_tag="VFC01_34845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN84835.1"
                     /translation="WARAHEIAAAIAAKPPSATQGTVKAIWESLDKPYRAAMEQNLIY
                     TRLGNSLGQAELAKGNGVQATPRVR"
BASE COUNT         4904 a         8717 c         8921 g         4987 t
ORIGIN      
        1 cgccaatcac accgacctac gacacggcca catcaccaaa atcgggcgcc acattttgca
       61 acagcccgct cagcgtacgt aaccgcgccg aaatcacttc gtcagtgacg cgtagcgcgc
      121 aacgtggtag tccgtcgaac cgaattcgta ctgcaacgcg gtgagccgct tgaagtaatg
      181 gccgatcgcc agctcctcgg tcatgcccat cccgccatgc agctgaaccg cctgctggcc
      241 cacgaagcgt gcggccctgc cgatggtcgc cttcgcggca gacaccgccc gcgcccgcac
      301 gtccggcgat gcctcgaggt tgagcaccgc gagatacacc gccgcaaccg cctgctcgac
      361 ttccatgtac atgtcgacca tccggtgctg cagcacctga aagctaccga tcggctgccc
      421 gaactgctgg cgttgtttgc agtattcgac ggtgtcggcc aacacctttc gcatacagcc
      481 caccgcctcg gcgcagatcg ccgcggcacc ctcgtcgcgt gcctgggcca gcgatggcga
      541 tgcctggccc tcctcgccca gcagtacgcc gcgcacaccg tcgagcacaa gatcggaagc
      601 gcggcgatcg tcgaccgtcc ggtagtggtg accgtcgatt gccgaccagt cggcgtcgac
      661 gagaaacagc gagagtccct gtggcgtgcg tgcggtgaca agcagatggg tggctaccgg
      721 tgcgctcatc gcgacgacct tcgacccgcg cagcagccac tcatcgccag cgcgtacggc
      781 caccgtggag acggcttgcc aatcgtcgcc cgatccaggt tcagtcgccg cgagcgccac
      841 cacggccgtg ccggccacga tcttctccag cagttcgttg gccaaagtgc cgccggctcg
      901 tctcagcaac ccgcccgcca ccacgacggt atcgacgtac ggttccaccg ccagcgcatg
      961 ccccagcgcc tcggcgatca ccatgacttc gactgggcca ccgccgattc cacccacttc
     1021 ttcaggtagc gcagcgccca ggatgcccag ttcgtcggcg aacccgcgcc agatgtcggg
     1081 ttgccaaccg gtcccggtct tggctgcagc gcgactcttc tcgaggtcgt atcgagtcga
     1141 caggaacttg gcaagcccgt cgcgcagtag ttcctgttcc ttgctcagat tgaagtccat
     1201 ctacagcccc aatgctgcct tggcgagaat gttccgctga atttcgttgc tgccggcgta
     1261 gattgagccg gccctgtcgt tgaagtagcg caacggtgcg accgcctgcc atgcctcgcc
     1321 gctgacgtag ccgtccggcg gcggctcgaa ctcggacacc ggccctcctg ggcacgtcgc
     1381 gtgtggttga tagacccggc cgcgcggtcc ggcagcttcc atcgccaatt cggtgagcga
     1441 ttggctcagc tctgtggcca gcaccttgag catcgacgca cccggcccgg gatgaccgcc
     1501 gccggcgagc gcggccagca ctcggtactc gaggatctcc agcacctcgg tgcggatgcg
     1561 ggcgtcggcg agcttgcgcg caaacgcggc atcgtcgagc agccggccac cgcccggtgc
     1621 gggttgattg gcggccgcag tggcgatctc ctcggccatc acctgcagcg cgggtgaggc
     1681 tgcgccaccg cctcgttcga actccagcag atacttcgcg acggtccagc cgtcgtcgat
     1741 ctggccgagc acgttcgact tcggcacccg cacctcgtcg aagaagacct ggttctggat
     1801 ttcctcgccc gacgtcatca ccagggggcg gatctgaagg cccggcgacg tcatgtcgat
     1861 caggacgaac gtgatgccct gctgcttctt ctgcgtcttc gaggtgcgaa caagggcgaa
     1921 catccaattc gcttcgcgag catgcgtggt ccagatcttg ctgccggtgc acaccaagtc
     1981 gtcgccgtcg tcgatcgccg ccatcgacag cgccgccaga tcagagcccg cctcgggttc
     2041 ggaataaccc tggcagaaga acacttcgcc ggtcaggatc cgcggcagga agtactcctt
     2101 ctgcgcgtcg gtgccgaact tgatgatcgc gtgggcgacc atccggatgc ccatcggcga
     2161 caacgagggc gctccggcca actgagactc gcggctgaag atgtagtgct gggtgaggct
     2221 ccagtcgcaa ccgccgtatt cgaccggcca ggcgggcgcg gcccaaccgc gttcgtgcag
     2281 gatccgctgc cactccatgc tcgcctcgtg gtcggcgtag acgctcgtca tgaggcggcc
     2341 agcccgccgc agtccgggcg tcagcttttc ggccaggaag gcgcgcactt catcgcggaa
     2401 cgccgagtcg gtcgccgacc atgccaggtc catcgatttc ccctgtctgc cggagcgcgt
     2461 tgcagacaga gtaaacctag ttagttaagt gggcgacatc ggggcccagg atcgctaccg
     2521 ctaaactctg agcaggtttc cggtgacgag gcacgccgac aacggccata atcgggaacg
     2581 tggagcggcg gcgacgagac ggcgaggaac gccaagatcc gatgaccctg ctcaaggaac
     2641 tcccggcatt ggtggtgctg gaacgttttc cggtgccggt tcttgccatc gcagaggatg
     2701 gcaccatctt gttcgccaat gaggcattcg gccagatgct tggctacccg gcgggcgtgg
     2761 tgcgggcgtt gaagtttcgg cagattttcc acactctgcc gaccgacgaa tccgccgtct
     2821 cagtggtgcg tgcccacgcg gacctcatcg tcgagctgct gcaccaggac ggttccaccg
     2881 ttcgcgcccg gatgagcaag tctgcgttgt tgcgtggaga cgacccggtg gcgttggcga
     2941 cgttccagga cctcaccgaa cgattgtggg tcgacgagct gtagtcagtc ggtcagtggt
     3001 gtgtcggagt gccgcagcga ctcgcgcgca aggaactcat cgaaatacga caacgactcc
     3061 tggttcacta tcgccggcag cagcactttc cacgtccgag cgacgcgcgc caagacgtcg
     3121 gctccggccg tagtcgcgtt cgcgagcaat tccgccccaa gcatcgagcc gacgattgtc
     3181 tcggcgaccg cctgtggatc gagttcggcg cgaagatcgc cctcatcaat cgcttgacgg
     3241 gtccgctccg tcatctccgc cacccaaccg ccatacacca gtgccgccgc gtcgttgaat
     3301 tcaccgaacg cccgcaggag ctgcgttccg cttcgggcga tcttgtcggt actcaataga
     3361 tctgcaacga cgaacactcc gtggatgatg ttttccaacg ccggggcaga ggatgcggtg
     3421 atgttcgcaa acgcagcgac caggtgggcg ctcgcttctt cgatgatcgc cgtcgcgagc
     3481 gattcctttg agtcgaagtg atagtacaga gcgcccttcg tcatttcggc acggtcgatg
     3541 atgtcgccca atcctgtggc cggataacca atttcgttga acaactcgac ggcggacgtg
     3601 atgatcttcc tgcgggtagc ctcggatcgc gcttggcgca ccattgtccg agtacctccg
     3661 gatgttatcg acctgcccag gatggtgatg gccggatagg gcatcctaat tcatcgcgtc
     3721 agagtggtgt cgctcgcttc aacgcgacca tagtgcgccg cttgatgaac ttctcgaggt
     3781 agtcgatcct gtcggggttg acgaaaccac gcagcatgag cacccacaag tcctccaagt
     3841 cgtgcaggaa ccgctccggc tcagccaagt tgctcgtctg ccgcagaccg aggtacatcg
     3901 acaccagcag ccttgcaatg ccttgcgggt cgcggtcgcc gatgacatcc ccgtctttga
     3961 tgcctttgcg cgcaatcgct gcgaaggccg ttacccactg cccgtggatg tgttcctgta
     4021 atccgtcagt gcgcccaatc gactcaagga gattcaatcc ggctcgagcg gtctcgtcgc
     4081 cgatgtcgtc gacggcgacg aggtaagaga tgtcgatcag cgtctccagg cccgagagct
     4141 tttgtgccag cagctggtcg accgaggccc tggccactgt gccgcggtgc tcgatgatcg
     4201 ccgacgcgag cgcgtacttg gaccggaaat ggaagtacat cgcgcccttg gtcacctcag
     4261 catccgcaag aatgtcgtcg aggttgacca agctgtacga cttccgtgcg aactgatgcg
     4321 acgcagcgcg gagaatctgc agccgtgtgg tgtccgctcg tctatcgagt tgttcggtca
     4381 cttgctggcc tcgaaatctg ggctatggct cctgcgggcg ggtaatcgtg atttgcgtaa
     4441 attgaacgat ctcccgattg tttcatctgc agcacggaaa aggcaggcgc acacgatttg
     4501 cgccgcagtc cgtgtcaacg accctcctcg atacgctcgg gtctcccgcc ctcagggcaa
     4561 acgaatcgcc aatactctaa gtcatgctta ggcctacata acagccaaaa agtatgtaag
     4621 gtactcgatg tgatgactgt tggtccggtc caagccgggc ggccgttggc ggctgaaccc
     4681 gaggtcagcg gtgccgtatc aacatcgatt gcgctagcac cgagcatttg ccagtggtat
     4741 tttcatgccg ttgccatggt ccgcgctggc aaaccccctc gaacccttgc gcgggctgtg
     4801 gcaactccct gtcggccgat cgcattacac gcccgatcgg ccgagcgtga ccatcaaatt
     4861 tgtctcccaa gcgcgtcccg tacggccgcg aagtctgctt cgtcggtttc ggtgtcgggc
     4921 gaattagcaa tacggaggta attaacggtg aactgggacg aagaagtcga cgtggtgagc
     4981 accggctccg gcgtcgctgg actggcgcac gccctggcag tcgtgaacat gggcggcgag
     5041 gttttcgtcg cggattcatg cagcgatgtc gacccaggac gaacctccgt cgcggtccgc
     5101 tcgcgcgtcg atcggctgca ctggctcgaa gtggacattg cggatccgga cacgaacgaa
     5161 tacttcgcgg ccctgtcgtc cgaccttggt ccgttaaccc gatccgcagg agacgtcgac
     5221 gtaccgattc ggatggtcga tcacgaggag cctgtcgacg cccgcggtcc cgttgcgccg
     5281 ttcatcggtg cccgcctccg ggattgggcc gcacgttgtc tggtgtcgcc gtacggatac
     5341 ctatacaccc gggtgtctga ttggcggtct accacgctgc gcacgctcga tggcgagtcg
     5401 cttcaggtcg ccgaaatcgg ctcgatcaca cccgatcccg ccaatgtggg tggctcagtt
     5461 ctggactggc tcagcacaca ggctcgggac cggtgcatcg agattaatcg ctctacctcg
     5521 ctacagcgca tcgtgttcga ggagggtcat gtagtggggg ccgtcttcac gactccggat
     5581 ggcccgctgg cggttcgtgc ccgccatggc gtgacggttg ccagcggtgg gtcccaggca
     5641 gccctagccg gcgagcagcc cttgccgctc gacggtggct cgctgcgcgt gtgtctggtg
     5701 ggccagacgg ccagccgctt tggtcgcgtc gagttgctga ctaccgaacc gttcgccagg
     5761 gatgccgcgt ccaactgccg tccggccaac cgtcagttac atgcgaacct gcacgcgacg
     5821 cacagcccgt tacagacgtg gcgctgcgga aaagtcaacg ggcactcgtc ctttggccaa
     5881 tagggccgcc atcgcgccac cggcgatggg gcgtgacaaa aggaacccct gcgcgcggta
     5941 acaaccgtgc cgcagcagtg tcagagctgc ggtctctgtc tcgacgccct cggcaacaag
     6001 gtggaggccg aatgcctcag cgagcgcgat gatcgctctg acgatcgcga gatcaccggg
     6061 gttggagcct aggtcgcgaa cgaacccctt gtcgatcttg agggtgtcga caggaagcga
     6121 cttcaggtgg gacagcacgc tataccctgt gccgaagtcg tcgatggcga cttggacgcc
     6181 ggcctccttg agtccggcga gggtgatccg agtggtctcg atgtcttgaa ccacaacgtt
     6241 ttcagttatc tccaggcaca ccgagccgcc gtcgaggccg aattcggaga tgatgccggc
     6301 gaccgattcg acgaagccgt cggtgaccaa ctgaaccggc gagacgttaa tgcgtagcac
     6361 tgtgtcgtgt cccaagccgc gggagcgcca ccccgcgaac tctgagcatg ccgctcgcat
     6421 cacccagcgg cccaattcgc ctgcgaggtt tatggattcg gccaccccga tgaatgagtc
     6481 gggagaaagc aggccgcggg tgggatgctc ccaacgcacc agcgcctcag cggcaagaat
     6541 ctcaccggtg cgcatgtcga cctcgggcag gtaatgcagc agcaacgagc cgttttcgat
     6601 cacactttgc aggtgaagct cgatgtcgtt gcgaaactcg ttttccatcg acatgtcgtc
     6661 agagaacacc gccactgtgt tgccgccgga attcttggcg gtgagcaccg cctgatccgc
     6721 acgccggaga aggtctgagg tggtgtcgcg tcctggaacg cccagtgcca caccgatgct
     6781 cactgtgcgg gtaagcactt cgccgtcgat agccacccgt tcgcacagcg tcgactgcag
     6841 gcgataggcg agcgcttcgg cggtatctgc gtccatcggc gtcgtcggca cgacgacgaa
     6901 ctcgtcgccg ccaagccggg cgatcaggtt cgggccttct gcgccttcgc gcaggcgttc
     6961 ggcgaggacg cggatgaacc agtccccggc ggtatggccg agatagtcgt tgatggcctt
     7021 caaccggtcc aggtcgaaga acaacgccga tacgggtccc ggctggcccg ctgcgagcct
     7081 cgcgtcgagg tgttccagca gcgcacggcg gttgtgcagg ccggtcaggt cgtcgtggtc
     7141 ggcaaggtag cgcagctgct cctcggcgag cacacgagct tgtacctggg cgaacagtga
     7201 agcgatcgcc ttgagcgcgt tgagttcctc ggatgtccag acgcggtctc cggcctcgac
     7261 gaagccgatg acgccggtgg tgacgtcgcc ggaaagcagc ggtacacacg ccatcgaggt
     7321 gaccgcttcc gtacggaaga cgacgggttc cttctgatac tcactgagcg caaaaaccgg
     7381 atcagcgtcg gcgaagtaga tgactgcgag cgggtcgggg tccgagatgg cgggacggac
     7441 cggccactcg gcgaccagta tcgaggcgtg gatgccgtga tcgttctgcc gcaggaagcc
     7501 gccgtcgaca tcgaagtact cgacgagatc agcgaggact cgttcactca cagccacgga
     7561 cgtggccgcc gataccccca tcaattgggt cgcgactgac gtgacgacca gctccaggct
     7621 gcttggcacc tgaccctccg tattggcgtc agctcagtgg tgacgaaact cgatgcctcg
     7681 cctgccccgg tgtccgccag cttgcggaga ttgtattgca gatcgcacgg gcgcctgcga
     7741 aaagcgggcg agaccacccg gagcggcggc ggcgcggtca gaatggcttg gcggtaaacc
     7801 cccagcgcaa caacgccgca tctcgacgcg cggggcccgg caacggcgtg tggcggccga
     7861 gcgaggcgct cgctttcgca ccagcggccc gcatccagta ggcctgcagg taagggagag
     7921 gtcttgcaaa tccgtactgg acgccaccga gtccggcggc agcgagctcc gacgacagct
     7981 ccgcgtgagt gaaggcggcg gcctcgctgg cgagcgcgtc cttatatgcg accgacctta
     8041 tcgaggggtc gacacaacgc agcatggccg aagcggtcga cgggtctcgc agccgctgga
     8101 agtcggaaat ccaaatcgcc gcgccactgt ggcggcgcac gtcagcgatt tggcgtaagg
     8161 cggcgcgcag accgtcgaag tccggtagct ggtgcagggt ccacatgcag gaaaccccat
     8221 tccaggctcc tctggccagc cggtcgggca gggcggtgat gtccgcatga accagttcga
     8281 cacgcccgtc gaagccctct gttctgagca ggtcttgcgc cgtggccagc atgttaggag
     8341 ccaagtccac agccgtgatt tcgaggtctg gccggcgacg aagaacggcg gccagagcac
     8401 ggccggaccc gacgccgaga tcgagcagcc gtgcaccggt cggcagcagt gcatcaaggg
     8461 cccgggagcc caagtcgtac accgcctgta tccctggatt ggatgcccca gcggcgtgga
     8521 attgggcgac cgattcggga tcgtccatca ccatcggttc aggggtccgt tcgcgtcccg
     8581 cagggtgacg acgttctcgg gcaacgatgc cgggtatccg ccagagcggt cgaaatccgt
     8641 tcacccttgt accttctgcg gggtctgtca gttggagtgc gcaaagctta gtaccttgcc
     8701 gcaccgcatt cctttgctga gtcatggata tgctgacaga aggggcagac cgtcggggag
     8761 gatacgacgt ggtcgagttg cggctggaaa gtacccttgc cgaactgccc ttggcgccga
     8821 agaacccgct gccgtacagg caacgacggc gcgccgccaa gtcgtttcac accggcatgg
     8881 aaacgttgcg tgatgcgggt gggccgataa cccgattcac gcttggtccc cgcttgctcg
     8941 cgccgccgct ggtgttggcg acctctccgg gcgcaattcg cgacattctg agcatcaagg
     9001 acgattccgt ggacaagacg acttccgtct tcgtcgagat gcgccgcgtc ctcggcgcga
     9061 atctaagcaa ccttccctac gaaccgtggt tatcgcgtcg acgcacgctg caaccggtgt
     9121 tcaccaagca gcgcgtgcgc gaattcggcg gccatatggc acaggccgcg gaatcggtgt
     9181 gcgacgaatg gcccgatgga ggcgtggtcg acctcgacgc cgagtcccgc aaactcaccc
     9241 tgcgggcact gggccgttcc gtgctcggga ttgatctgga ccatcgggcc gacgaggtcg
     9301 accagccgct gcgcgtggcg ctcaaacatg cgatgcaccg tgcgattctt cctgtgcgcg
     9361 tcccgagctg gctgccgata ccgggtgccc gccgcgcgcg ggcggcaagt accaaagtgc
     9421 atcgcctggc gcaggagatt ctgcgctcct gccgcgccga ccccaccagg gttgcgccgt
     9481 tggtccatgc cctgatcgac gcgaaggacc cagtcacggg agagtcgctg tccgacgagg
     9541 agatctgcga ggagctgatc atcttcttgt tcgccggcca cgacacgacc gcgaccactc
     9601 tcgcttacgc gttgtgggcg ctcggccgca accccgagtg ccaggaccgc gtcgccgcag
     9661 aagtcgccga acttcccgat ggcccactga cggtcgacga tatgccacgg ctgaactaca
     9721 ccgtccaggt gctgcgggag tcgctacggc tgtgccctcc ggggcccacc ggaacgcgca
     9781 tggcatgcag ggacattgaa gtaggcgggt accgggtgcc ggccggcacg atgctggtcg
     9841 tcggcaggat ggccgtgcaa cgcgatccgg ggctgtggga tgctccgttg aagttcgacc
     9901 ccgaccgatt cagccccgac aacatgaaga aacttgatcg gtggcaatac attccgtttg
     9961 gcggcggtcc acgctcctgc atcggtgatc acttcgcgat gctcgagacc accttggcct
    10021 tggcgacggt gatccgccgg gccgaaatcg actctgtgga agaggaattc ccacttgccg
    10081 tgcacttcac gatgatcgcc ggtgctccga tccccgcacg cgtccgaccc cgccggcctg
    10141 gagtcagcgc gtgatcgcga ctgatgaggc gggggtattc agcgcccagc ttctgaatcc
    10201 cgccgatgcc gacgacagcg ttttgctcga caggttgcgg caagactcgc gcatcgaatt
    10261 caccgatcat cgcgccgaat tgctcaaatg cctgcggctg ctttgcccct cacccgacgc
    10321 cgacatcctt tccgagccga cctcatgggc ctactacccc tggcgtcgcg cggtggttgg
    10381 tgtgctgggc cctcgggcgt accgcgcgct tcggttggac cgcaacagac atctgatcac
    10441 cgtggccgaa caggatcgcc tcaacgggct tcgaatcggc gttgcgggat tgagcgtcgg
    10501 gcacgtcatc gcgcatacgc ttgcggcgca aggcttgtgt gggacagtgc gcctcgccga
    10561 cttcgatgat ctcgagctca ctaatctcaa ccgcgtgccg gccactgcgt tggacatcgg
    10621 ggtcaacaag gctgtcgtgg cggcccgccg cattgctgaa ctcgatccgt acattcacct
    10681 cgacgtgatg acggccgggg tgaccccgga atcgctgtcc gacttccttg aagacattga
    10741 catcgtcgtc gaggaatgcg attcgctaga catgaaggtc atgatccgcg aggcagcgcg
    10801 cgagcggcgg ttgcccgtgc tgatggccac cagcgaccgc ggtctggtag acgtcgagcg
    10861 ctatgacctg gagccggacc ggccgattct gcacgggctg ctcggccagg ttgatgcctc
    10921 gctgctggcc agcctgccca gtcgggacaa gctgccgtac atgctgagcc acatcaacgc
    10981 cgcccaaacg tcgccgcgtt tgattgcgtc actcgtcgaa gtgacaaaca cattgtcgac
    11041 ctggccccaa ctcgccgccg aggtgacgct cggggcggct gcagtcgccg aggcagtccg
    11101 cagaataggg ctgcagcagc ctctgagatc cggccagacc cgaattgaca tcagtcaggc
    11161 gctcgatgac gtcgctgagc cacccgcacg catcgacgtg cagcggctgc atgaggaacc
    11221 agcggacgac gaacagttgg acggcacgcc gaccgaaccc gtcgccgctg tcgcccaggc
    11281 cgcaatgcgc gcgccgtcgg gcggcaacgc ccagccctgg catgttgagg cgcaggccaa
    11341 ttcggtcgtc atccgactcg cgccggaatg cacctccatg atggacgtcg ggttccgtgg
    11401 tagcgccgtt gcgatcgggg cagccttgtt caacgccaga gtcgccgccg cggcctggaa
    11461 cgttctgggc ccgacggtat ggaaaaccga cgatgtgacg tgtccgctgg aggttgccct
    11521 gcgactcgac cacggcggcg acgcggaatt ggcggacctg taccagccga tgctgcgccg
    11581 agaaacgaac cgtcacctcg gcgtttcaga accgatcggc gcagaaacgg tagcgctgct
    11641 gcacgacgtc gcacgccggg agggagcgcg gttgtgcctt ctacaggcac gcgaagactt
    11701 cgaaaaagtg gcgagcatct tcgctgccgc cgatcggatt cggtacctga caccacatct
    11761 gcacaaagaa atgatttccg aactccgctg gccgggagac gaacccgccg acaccggcat
    11821 cgacgtccgt agcctcgaac tcgatcccgc agagctcgcc gtcctggacg ttcttcgccg
    11881 cgcagatgtg atggccgaac tcgcgcaatg gaacgccggc gaggcgctgg gcgacgaggt
    11941 ccgaaaacga atccgcgcat cctcggctgt cgcagtgata acggtgcacg gccatgaact
    12001 catcgacttc gcctgcggcg gatcagctgt cgaggcggtg tgggtcgtgg cgcaacggct
    12061 cgggttggcc gttcaaccga tctcgcccgt ctttcttcac gcggtatatc gcgatgaaat
    12121 tgtggaactg tcgccgtcct tcgccacgga gctgcagaac ctccatgcgg attttcgtca
    12181 gctggcgggc acgaggaacg acgaatcgca agtactggtt ctgaggttcg cccatgctcg
    12241 cggggcatca gtgcgcagca aacggcgcgg cctcatactg aaatgaatcc cactggccga
    12301 caaggagcga caccatgccg gcgcctttca acgacatcaa cgcgacccac atccgctcgt
    12361 cgacaaaaac gtctctcgca tcgcgggact gccaattctg atgatttccg gccagcgccc
    12421 ttctgctgcc gccgaacggc ctctcgcgga ctccagagca aagcggacct aactgtgctg
    12481 gattcggcgt gcgagtagcg tgtaacgcgc atgggtcagc ctaggagaga gaagtgccgc
    12541 gcagcctcga ggttgtcgtt actgaggtcg ctacccgctt aattggggcg accgcggcga
    12601 cgcaaccacg cgtaagtcaa gaggtgatgg ccgagctggt cgatgtctta ggcgtcgatg
    12661 cgagcttcct gcgccacaac gaccacgcta tccgggcgac caagttgatc gcggaatggc
    12721 cggtgcgccc ggacattccc gatccggacc cgctcgggac agtgttcttc gccgacgcgg
    12781 acccgatctt cgccgccgcc gaacacgcca aggaaccgtt gattctgcgg ccagatcctg
    12841 cctccgatga ttaccagcgc cgtgtcgagg agggccgcgg ggttccggcc atctcgatgg
    12901 cgtcggtgcc actgatatcg ggagacgtca ccacgggggc gctcgggttc gtcaagtttg
    12961 gtgacaaatc gtggcgacct gaggagatca atgcccttaa ggcgatagcc tccctgttcg
    13021 cccaactgca ggcccgcgtc gttgcggagg aacagttgcg cttcctggca gaccatgacg
    13081 atgtgacggg gttgcataac cgtcgatcgc tgctgataca ccttgacgaa cgattgtcgc
    13141 cgggacgacc ggggccggtg gcgacgctat tcattgatct cgaccggctc aaggcgatca
    13201 acgactatct cggccacacc gccggcgatc aattcatccg tgtgatgagc gaacggctca
    13261 gagcgggaac cgggggacag cacctcatcg ctcgcctggg cggcgacgaa tacgttgtgg
    13321 tgccgaccgg accaaccgac gcagaggcgg cggaagcact tgcggacaaa ctacatagca
    13381 tgctggtcga gcgggtgatg ctcggcagcg agatgctcgc tcgcaccgtc agtattggcg
    13441 tcgcggttgg gacgcccggg ctggacacca cctcggatct gttgcgcaag gcggatcaag
    13501 cggtgctcac ggcgaagaat tccggcggga accgggtctc ggtgttctcc gatgacatgt
    13561 cgctgacgag cgaattacgc aacgacatag agcttcatct gcgaggcgtg atcgagagcg
    13621 gcgccttggt gcttcactac ctgcctgagg tcgatatgcg aaccgggcaa gtcctcgccg
    13681 ccgaagcgct ggtgcgttgg cagcacccga cacggggtct gctgatgccg gatgtgttcc
    13741 tcagcgtggc ggagtccatc aatttcgcag gagagctcgg ccgctgggtc atgcggaatg
    13801 cgttcgcgga gttcagcaga tggcggtcgc agcgtgtggg gcaagacgcg ctgctgcgcc
    13861 tcaacatctc accgattcaa ctggtcggca gtgaattcgt ggaacacgtc gccggtgtgt
    13921 tggacgagtt cggcctcgac gccgggtcag tatgtcttga agtcaccgaa agtgttgtgg
    13981 tcgaggacat ccagaccgcc cgggtcactc ttgcgaggct gaaggagatc ggtgtgcaga
    14041 tcgcaatcga taacttcggc accgggtaca gcgtactgtc gcaattgaaa tcgttgccgg
    14101 tggacatgct gaagattgac aagagcttca tccgcgaact cggctccaac cctggcgata
    14161 tggcgatcgt taaggcaatt gtcgcgctgg cgaatgcatt tgaactggaa ctggtcgccg
    14221 ccggggtcga gacgcaggta gccgcggaag cgttgcttga gctgggatgc tatcgggcac
    14281 aaggctatct gctgtcgcga cccctgacca gtgaggcaat gaagttgcta ctcaccgaag
    14341 gccatttgca aatacctcct gtcaagccgc ggggcaagtt tcttctgctc ggcaaagatc
    14401 attgaaagct gtttggggtc agcgtgtttg gcgaatgtgc tgcgatccca ccatgctagc
    14461 ttcgtccggt agcggtgatc tggataggga gtggcgggaa ttttggtcgc cagcagtccg
    14521 cctgacgaga gccactgctt gaggacatgc tcggccgccg tcgccatgat gaactgaatg
    14581 tcgagcaggt tcatcgtatg agaggaggtg cgtgccatgg cggtggtgag tagcccatta
    14641 cgctcggaat cgtctgtcac ccaagccgtt ttcatttcca ccacgccaaa cggcagtcga
    14701 tcgctgacca ttttgcggac agcatcctga ccgggctggc cattccactc aagaatcgcg
    14761 tgacattcat cggttgacct atacgggccc ctaccacgca cgccacccac gaccctaccc
    14821 gcgccgtcga cgcaggctac gaaaaggatt gtgtcggcgc cgctgcgaag cgcatccatg
    14881 tcgagtgcct tctcgactcc atgcttgcgg tagctctggc acgcgccgcg aacgtagtcc
    14941 gcccacagct ctcgctcaac cgccggctgg gcgaatacga tcctgcattc gctatcgcga
    15001 tcccaatagt tgggagtgtt cttcaggctg acaacgatgc ggctcgcggg ctcagtctgg
    15061 ggaaccgtca tgcttgtccc ttcctcgggc agccggccaa caacggcgtc gcggcggctg
    15121 gctagaacat cagtatcacg ccccgaatca ctcggattcc agctagcaat acagtctttt
    15181 cctgcaccgt gatacctgct cgcaaatttg gcggggcgca gcaggctgtc ggcggcttgc
    15241 ggcaatctaa cgctcagcga attaacactg gtgatcagct atttgtcgaa ttcttggcga
    15301 agttggcaga cgacatgatc aattcggttg acgccagcgt aaggattccc aagaggcttt
    15361 cgctatgcgt tgtttgagcc agtagaattc ggctttggtc ggccgtcggc gggaccgtcg
    15421 cattactggg cgttatttgc tgtcagccga ccaccgacgt tgagcggccc gaacggcctg
    15481 cctgaccgcc gctggccggc gcaaaatgtc gagatttcgc ggccggcggc ctgctgaggt
    15541 gacaccctga agcaggtgga cgaggggtac gcgaccgcag gtcgcgggcc ggtcaaggat
    15601 gtgggccggc tcctgctgcg ttgtgccgat cgaccgggcc tggtggcggc ggtaagtgca
    15661 tttctcgcgg acgccggcgc caacatcgtg tcgctggatc agcactccac tgagcagtcg
    15721 ggcggcacgt tcatgcagcg gacgatattt cacctgcccg ggctgacggc cgcgcgcgac
    15781 gcgcttgaac gggattttcg ggaacaggtg gcctcgcagt tcgatatgga tttccggttg
    15841 accgaggctg cgaagccaaa acgggtcgca atcatggcgt caaaggaaga tcactgcctg
    15901 ctcgatctgt tgtggcgcaa tcggcgcggc gagctggact tgtcggtcgt gatggtgatt
    15961 tccaaccatc cagatctggc cgaccaggtc cgcccttttt cggtgccatt cctgcacgtg
    16021 ccggcgacca aggatattcg ggaggaagca gagcgccgcc agctcgactt gttacgcgga
    16081 aacgtggact tggtggtgtt ggcgaggtac atgcagatcc ttacaccgca atttctggcc
    16141 gaagtgggct gtccgctgat caacattcac cattcatttc tgcccgcctt cattggggcg
    16201 gcgccgtatc gcagggcaaa ggaacgtggc gtcaaactcg ttggcgcgac agcccattac
    16261 gtgactgaag acctcgacga gggcccgatc atcgaacagg acgtggtgcg tgtggatcac
    16321 cgccacggcg tcgatgatct cgcgcgttta ggcgccgacg tcgaacgcct tgttctgtct
    16381 cgggcagtgc tatggcactg tgaggaccgc atcatcagat acggcaacca gacggtcgtc
    16441 ctctagagcg gttaaggaga taagtggtga agaagttcag cggactggac gagttcgtcg
    16501 ctgcccaggg cagtcagctc ggtccgaccg attggctgga ggtcactcag gaccgcgtca
    16561 acctattcgc ggacgccacc gatgaccatc agtggatcca cgtcgatccc gagcgtgcgg
    16621 cgaacggccc gttcggtgga acgatcgcgc acggcctact gacgttgtcg ctgttgccgc
    16681 acttcacaca ccagctttac accgtcgaca acatcgcgat ggccatcaac tacggctaca
    16741 acaaggtccg cttcatcact ccggtgcggg tggggtccaa gattcgggcg cgcgcggaga
    16801 tcgccaaggt cgatcaactg gaaggcgcgg tacaggcgac cgttacggtg accgtcgaga
    16861 tcgacggttc ggacaaaccg gcggccgtgg cggagtcgat cgtccgcttc atcggctgag
    16921 cgcgtcagta ctgcgtcatg ccctggtcga tcgagatggc cgccgcggtc acgtttttcg
    16981 actcctcgct ggccagccag cacaccgcgt cggcgatatc gtccggttcc gccgcccagg
    17041 cgggtagaaa cggtgtgatc atctgggcca gctgcggata ggtctcgatc gcacggccca
    17101 gcgagccgat catgtcaccg cttcccatca gcgtgttgac cgctccggga tgcacgctgt
    17161 tgacgcgaat cgagtgcctg cccaattctg cggcgaacgc gcgcgccatc ccggtcaccg
    17221 cgtgcttgct cgcggtgtag tgcaccatga acggctgcat cttgatcccg gcggcagagc
    17281 tgatcaggat gatcgatccg ccacgcccgc cgtcgatgat cttctgggcg cctgccatca
    17341 cggtgttcca ggcgccggtc acgttgatgt ccatgacatc gcggaaatcc tcgggggtga
    17401 tggcatccca cgtctgggga gcggacacac cggcattggc cacgatgatg tccagccggc
    17461 ccagtgcggc aacgccgtcg tcgacggcct cgttcagccc cgcgacgtcg cgcacatcga
    17521 cgacggaggc gaggatcttc cggccggtgg cttcgaccaa gttcacggtc tcggcgagat
    17581 catcgggagt ggcggaatca taagggacac agggtggaag cttgcccgcg atgtcgatgg
    17641 cgatgatgtc ggcgccctcg gcggccatcc ggaccgcatg ggcgcggccc tggccgcgcg
    17701 ccgcgccggt gatgaacgcg accttgccct cgagccgtcc gctcacttct gctccttctg
    17761 cgccgccttg accagattcg atccgatgat cagtcgctgg atttcgctgg tgccctcgta
    17821 caagcgcagc agccgcacgt cgcgatagat ccgctcgacg ggaacttcgc gcatgtagcc
    17881 gctgccaccg tgcacctgca cggcgagatc ggctaccttg cctgccattt cggtgcaata
    17941 cagcttggcc gccgacggtg cgatccggcg gtcctcaccg cttacccaaa ggcgcgcggt
    18001 gtcgcgcacc agggctcgcc cggccagcac gccggtctgc tgatcggcga gcatcgcttg
    18061 caccagttgg aagttcccga tgggagtgcc gccctgtgtc gcggtggcgg cgtacgcgac
    18121 ggattcgtcg agggcgcgct gcgcgattcc gacggccagt gcggcgatgt gtacacggcc
    18181 gcgcgccagc gaggtcatcg cggcccggta tccgatgtct tcgcttccgc cgatcagcgc
    18241 ctcatcgggc acgcggacgt cgtcgaaact gacgtcggcg gtccaggcgc cttcctggcc
    18301 catcttggcg tctttcgccc cgacgacgac acccgacgag tcggcgggca ccaggaatac
    18361 cgcgatgccg ggcccgtcgt cgtcggcggg ccgggtgcgg gcgaacacga tgaacaggtc
    18421 cgccatcggc gcattggtga tgaagcgctt ctgccctttg atgatccaat ctccgccgtc
    18481 gcgaacggct ttcgtacgta accccgacgg attcgatccg gcgcccggtt cggtcaacgc
    18541 gaaggaggcg accagccccg acgccatcgg ctccaaccat cggcccttct gttcgtcggt
    18601 gccgaacccg acgagcacct gcccggcgat gccgttgttg gtgccgaaca tcgagcgcag
    18661 tgccagcgat gtgtagccca actccatcgc gagctcgacg tcttgggcga gattgagccc
    18721 gaggccgccc cactcctgcg gaatcgcgta gccgaacagc cccatctttt cggcctgctc
    18781 gcgcagatcg tcgggcaccc ggtcctcggc cagaatttcc tgctcacggg ggaccacagc
    18841 gctgcgaaca aagtgacggg tctgggccag gatctcctgg aagtcgtcgt cgctgacttc
    18901 ggtggtcatc gcgctcctct gcggcgagtg gggccagggg ctcgagaaat catatatgaa
    18961 atatgatgtt cgattgacag ggccttgatt ggggcctgac cagcgaagag gggtgaacag
    19021 gtgtcgttgc tgaccggtca aaccgcggtt gttaccggag gtgcgcaggg tctggggttg
    19081 gccatcgcgg agcggttcat ttcggagggc gcgcgggtgg tgctgggcga tctggacctg
    19141 ggtgcgacgg aggcggcggc caagcaactc ggcggtgatg atgtcgcgct ggcggtgcgc
    19201 tgtgatgtga ctctggccga cgaggtcgat gctctggtga ccgccgccgt cgagcggttc
    19261 ggcggcctgg acatcatggt caacaacgct gggatcaccc gtgacgcgac gatgcgcaag
    19321 atgaccgagg agcagttcga tcaggtaatc gcggtgcatt tgaagggcac gtggaacgga
    19381 accaaggcgg ccgcggcgat catgcgcgag aacaaacgcg gagcaatcgt gaacatgtcg
    19441 tcgatctcgg gcaaggttgg tctggtcggc cagacgaact actcggcggc caaggccggc
    19501 atcgtcggtc tgactaaggc cgccgccaag gaacttgccc accttggtgt gcgggtcaac
    19561 gcgatccaac cgggattgat caggtctgcg atgacagagg ccatgccgca acacatttgg
    19621 gaccagaagt tggccgaggt gccgatgggg cgtgccggtg agcctgccga ggtggccaag
    19681 gtcgcgctgt tcctcgccag cgatctgtcg tcgtatatga ctgggacggt cctcgaggtc
    19741 acgggtggga ggcacatctg atgcgcgacg cggtcatctg cgaaccggtt cggacgccga
    19801 tcggccgtta tggcggcatg ttcaagtcgc tgaccgccgt cgacctcggt gtcgccgcgc
    19861 tcaagggtct gttggagcgc accggaattg agccggctgc cgtcgaggac gtcatcctcg
    19921 gccactgcta tcccagcagc gaggcaccgg cgatagggcg tgtagtggcg ctggacgccg
    19981 gcctgccggt gacggttccc ggcatgcaga tcgaccgccg ctgcgggtcg ggtctacagg
    20041 cagtcatcca ggcggccctg caagttgcca acggcgacaa cgatgttgtg gtcgcgggtg
    20101 gggccgagag catgagcaat gtcgccttct attcgacgga catgcgctgg ggtggcgccc
    20161 gcggtggcgt gaaggtgcac gacggcctcg cgcgcggccg caccaccgcc ggcggccggc
    20221 actatccggt gcccggcggc atgctcgaaa ctgcggagaa cctgcggcgg cagtacaaca
    20281 tcccgcgcca ggaacaggac gagctcgccg ttatctcaca ccagcgagcc gtggccgcgc
    20341 agaaggacgg catcctggcc gaggagatca tcccggtcgc ggtacgcacc cgccagggtg
    20401 aggagttgat cgacatcgac gagcatccgc gtgcggatac ctcggtcgag acattgagca
    20461 acttgaaacc cgttctgctg tgcgatgatc cacaagcgac ggtgacggcg gggaattcca
    20521 gcggccagaa cgacgccgcc tcgatgtgca tcgtcacgac cccacagaag gctgacgagc
    20581 tgggcttgac cccgctggtc cggctggtct catgggggtc ggccggtgtc gcgcccaaca
    20641 tcatggggat cgggccggtg ccggcgacgg aagtggcgct cggcaaggcg agcctgaagt
    20701 tgtccgacat cgacctgatc gaactcaacg aggccttcgc cgcgcaggcg ctcgcggtga
    20761 tgcgggaatg gaagttcggg gcggccgacc tcgaccggac gaacgtgcac ggttccggga
    20821 tctcgctcgg ccacccggtc ggcgccaccg gaggtcggat gcttgcgaca ttggcgcgcg
    20881 agctgaaccg ccgcggcgct cgctacggcc tggagaccat gtgtatcggt ggtggacagg
    20941 gtctggcggc cgtgttcgaa cgggtcggcg catgacgcgg ctcgcacaga cgctcggcct
    21001 taccgagttt cagaccgaaa tcatcaccac ggtacggcaa ttcgttgaca aagaggtcat
    21061 cccgaccgcg caggagctcg agcacagcga tacgtacccg caagccatcg tcgatgcgat
    21121 gaaggacatg ggcctgttcg gcctgatgat tccggaggag tacggcggac tcggggagtc
    21181 actgttgact tacgcgttgt gcgtcgagga gcttgcgcgc ggctggatga gcatctcggg
    21241 cgtgatcaac acccacttca tcgtcgccta catgattcgc cagcacggca ccgaccggca
    21301 gaagcagcat tacctgccgc ggatggcgac aggcgagacg cgcggcgcgt tctcgatgtc
    21361 cgagcctgaa ctgggctccg acgtcgccgc gatccgcaca cgggccacgc gcaacgccga
    21421 cggcacgtac accatcgacg gtcagaagat gtggctgacc aacggcggca gttcgacctt
    21481 ggtggctgcg ttggtgcgca ccgacgaggg agccgacaag ccacaccgca acctgacggc
    21541 atttctgatc gaaaaaccca cgggtttcgg tgaagtcgcg ccggggctga cgattccggg
    21601 caagatcgac aagctcggct acaagggcat cgacaccacc gagctgatct tcgacggttt
    21661 cgtcgcgagc gccgacgaca tcctcggtga ggcacccggc cagggcttct tccagatgat
    21721 ggacggcatc gaggtcgggc gggtcaacgt atcggccagg gcctgtggtg tcggcattcg
    21781 ggccttcgaa cttgcggtgc ggtatgccca gcagcgccac accttcggca aaccgatcgc
    21841 cgatcaccag gccattgcgt tccaattggc cgagatggca acgaaaatcg aggcggcgca
    21901 cctgatgatg gtcaacgccg ctcggctcaa ggattccggt gaacgcaacg acgtcgccgc
    21961 aggcatggcc aagtacctgg cgagcgagtt ctgcgcggaa gtgacccagc agagcttccg
    22021 catccacggc ggctacggct attccaagga atacgaaatc gagcggttga tgcgcgacgc
    22081 gccgttcctc ctgatcggcg agggcaccag cgagatccaa aagaacatca tcagcaagcg
    22141 agtgctcgcc gactaccgga tctgactcat gaccacaccc gatttcgcct tccggcccca
    22201 actggccgag gacgtcgcgc gttacgtgcg taagcgcatt ttcgacggca cttatgctgc
    22261 ggggcagtac attcgcctgg atacgctggc tgcggaattg ggtgtcagtg tcactccggt
    22321 gcgagaggcg ctgttcgtcc tgagcgcaga gggcctgctg tcgcagcagc cgcggcgcgg
    22381 ttttgtggtg gtgtcggtga cgggtcgtga tctcacggac gtggcgaatg tgcaggcgca
    22441 catcggtggt gaactcgccg cacgagcagc ggtgaacatc accgaccagc agttgcgcga
    22501 gctggaaaag attcagacgg aactcgaaga cgcctacgcc agcgatgacg acgaccgcgc
    22561 ggtgcggctc aaccacgagt ttcacaaggc gatcaatgtg gctgccgatt caccgaaact
    22621 cgcgcagctg atgtcgcaga tcacccgcta tgcgcccgaa tcggtgttcc cgaccatcac
    22681 gggttggcca gagcagtcaa cggaccatca ccggaaactg ttggcggcgc tcgagaagca
    22741 cgacgaaggc cttgcgcgca tggcgatgtc cgagcacctc gccgccggtg cggcgccatt
    22801 gatcgaacac ctcacccagc gcggggtggt cgactgattc cggcgagcag acacaaaagt
    22861 gtccatttcg ccggcgtgtc gggcatcttt acgttccccc ttgcacgcgg ggtgggcccc
    22921 cagcttgcga ggggagtgcc cccagctcgc gcaagagagc ccgtagccta cggacgtcga
    22981 ccttgcccgt cgtgccgcga ggaatgtcat catcggactt caccgtcagc catactgccg
    23041 gaactttgaa ggagctcaac cgctttcgtg cttcagcgcg taggtcgtca accgtgcgtc
    23101 cggagattac gacagcgccc accgcgcccg acacgtcggt gatgaacgca ttctcgacac
    23161 cgtcgatgct ccgcagcgcc tgttctactt cgctcgggta gaccgtggcg ccgctgacct
    23221 tgaacatgtc atcgaagcgg ccgtggtaga acatgaagcc gtcgtcgtcg aggtgtccca
    23281 gatcgccggt ggggtagtag ccgtccgccg tgaacaggtc ctcgcggctg cgccggcaga
    23341 tgccccgcag cgtgtgcggt cccctgagct ggatcatgcc gatggtgccg gcgggaacgg
    23401 ggtggccggt gtctgggtcg acgatccgaa cctccatgcc gtcgaacggc ttgccgcagc
    23461 tgccccacgc ggtacgcggc atgtccgtgt cggcgggata gccgcagtac ggtccgaacg
    23521 actcggtcat cccgaacaac ttcgcccgcg cgccgggctc ggcgcgttgc tcgggcggga
    23581 gcagcgcttc caggctgcct ggccggagtg cggacaaatc cgctccgacg gaagcggatt
    23641 ggcgtgcaag cgcttctgcc tggtcgggcc agccgcgaaa cagcgtgacc cgatcacgtt
    23701 ccagtagccg cagcgtcgtc tccggccggg ggatctcctc ggtgaccagt gtggcgcccg
    23761 ccaatagtgc ggacaggatg ccgctgccga agccgccgac ccagaagaac ggcatcggca
    23821 ggtacagccg tgtgtccgcg tcgatgcagc gggcggcgag gccggaccgc acggccgcca
    23881 acgcgttgcc gtgcgagtgg atgacgccct tcggcggtcc gctgcttccg gaggtgaaca
    23941 tgatgaccaa cgcgtcgctc ggcgtaacgg ccgcagccat ctcgtcgacc atctgaccac
    24001 cgtgcgatcc agccacgtgc tcgggcatcc aaaccgtccg cagcgcaggg agttccgggg
    24061 tattcacgac gtccgccaga tcgtcgagat atcgatggcc gcggaactcc tcgaccgtga
    24121 tcagagcctg gacggaggcg gcccgcagct gggcaatcag ctcaggaggc tgcagcagcg
    24181 tgctcagcgg caccagaact gcgccgatgc gcgtcagtgc catcgcgagc tgcacccact
    24241 ggacgcggtt cggcatgatc agaccgaccc gagtcccctt ggcgacgccg gcctcgatga
    24301 acgccgcggc aagggcacgg gtgcttccct cgagctcggc gtagctgatc cgggagtctg
    24361 tgtcgatgac catcggcttg tcgttgagct gttgcgcgcg ggtcctgacg agccagtcga
    24421 tggtgtcagg catcgaacac cttctgtagc gcggtgaggt cgattttgcc gctggagagc
    24481 accggaatct cggaacgtgg cacggcggca aagcgtttcg gaatcttgta gacagacagc
    24541 tcggccttga gccgctcacg caccgccgcc tcgtcgaagt cagcgccgtc ctccaacgcg
    24601 acgatcgcgg cgactacctg tccgcgctcg ggatcgggca gcccgagcac gtgtgcgact
    24661 gtgccgccgg tcaccttggc gatcgcacgc tccacttcag cgggtgcgac gttggcgcca
    24721 gcagtcttga tcatggcgcc gcggcggccg agaaagtaca caaatccgtc ggcgtcagtc
    24781 cggacgagat cgccggtgtg gaaccagccg tcggcgtcga agcactcctc gcggctgcgc
    24841 ttgtaatacc gctgcatcac gaagggacca cggatgcaca gctcgccgtc gaggatcctg
    24901 gtttcgaagc ccggtgccgg cctgccgaac gaaccgcgcc ggtgttcggg ttggtcggat
    24961 tcgtcgtcac tgatggtgat gacgctgccg gcttcggtca tgccgagcat ggtgtgccgc
    25021 aactcgggat cggccggtcg cacctcgggt gccatgatcg ggtacaagtt gccgcgccgc
    25081 agagacgaca ggtcgcgctg ggggaggctg gggtggtggg cgagctgggc gatgccggcg
    25141 acgaaaccgt tggtcatggt gggcttttcg gactcgagca gatccagggt ctcgcccgca
    25201 tcggtggcgt tggagcacac cagcgtcgag ccggccagca gggtggccag caccgcgaac
    25261 gcgatcccgc cgatccagaa gaacggcgag ttgcagaaca gcttgtcgtc ggccgtcaac
    25321 ccacggatct cgttgagcgc cttctggtgc cccagcaggg atgcgtgcgt gtgcaccacg
    25381 cccttgggtg tgctcgtcga gcctgacgtg tagacgatcg ccagcggatc cgattcgtcg
    25441 acgtcgtgct ccatcgcttc gagcagcgtc gcatcggccg tgttgccggg tgcggagtcg
    25501 ataaggacgt ggcgcaacag cggggcggct tccttgatgc cggcaagcct ctgccggtag
    25561 tcgtgcgagc ggaacgacgc agtggcaagg aggatttcgg tgtcgctgtc ggcaagctga
    25621 gtggccagct ctgggacggt ggcgaacgtc gagaacggga cgacgacggc gccgatgcgc
    25681 gccgcggcca gcatcgctat gatccacgcg gctccgttcg ggaacagaac gccgacgtga
    25741 gttccttttc cggcgccgag gtcgatcaac ccgcgggcca gctgcgccga ccggaagtcg
    25801 gcctccgcgt aggacaggcg ctcggcgtcg cacaccagca gcgtgtgctc tcctccggcg
    25861 cgggcctgcc gccgcagaac ttcgccaaca gtcagcgttt cagttggcat gttcggcggg
    25921 gtggctgaag aactcccgga ccgcgccgag gtcggccttg ccggaaggtg tcctgggaat
    25981 ctggtccacg atcgcgatat cggtcggaat ctcgtagcgc gccaaccgcc ctcgcaggaa
    26041 ctcgatcagt gcgccggcgt cggtcgtatc ccgcagctcc accatcgcga ccggggcctc
    26101 gccgaggcgg tcgtccggcc ggccgatgac agccgcccct cgcacggcgg gatggctctc
    26161 caacgccgtg cgcacgtcgt cgggcatcac cttgaagccg ccgcggatga tcgcctgatc
    26221 ggcgcggccg agtatccaca ggaagccgtc cccgtcgatg cgcgctatgt cggtggtccg
    26281 catccagtcg gcggacggtc cgagctggcc gggtttgacc tccagcagcc cggcctggcc
    26341 gggccccagt ggcgtgccgt cgtcgtcgac cacccgcagc tgtgccccga ggctggcctt
    26401 gcccacactg ccgcgcttgt cgtgccagtg cttctggtaa tcggaaagtg tccagccggc
    26461 gacaccgccg ccgaattcgg ttgcggcata agaggtcagc acggggattc cgaacttggc
    26521 ggtgaatgcg tcggcatcct cggctgacag cggcgcggta cccgacgtga cggcgcggat
    26581 gctcgccagg tcgtcgcggg tcaagtccga gtgcaacacc atgcgcaggg ccgccggcac
    26641 cagcgacact gcgcgcggcc gatgcctgcg cacggcatcg gcccactgat tcagctcgaa
    26701 acggttcagc aacgcgaacg gccttgcctc gcatacgcac tgcagcattc ggaatacgcc
    26761 accgatgtgc actagcgggg cattgacgat cgcgacaccc cgccgcagtt cggtcggcgg
    26821 gggcgatgtg atgacgctgc gcgccagcat gtcgtaggtg aggtcgattc gtttgggcgt
    26881 acccgttgtc ccgctggtca gcatccgcac cgcgactccc ggtcggccgg cgccacgggc
    26941 gggcatcgct ggcgcagcgt ccagacccga gatcggaatc gccgtcgggc cggcggaaac
    27001 catcgacgcc aggtcgtcgg gttcgccgac tactactggt aggtccagcg cagcgatgtc
    27061 cgcgcgggta cggtcgtcgc cgcgggatgg gttgatggtg acgacggtgc cgccgccgag
    27121 cagcacgccg agaagggccg cgacgtgggt gggggtgttg cgcaacaaca tcccgacctc
    27181 ggtctcaccc acctcggcga tccgattcgc aagttcagcg acctgccgcc atgtcgacca
    27241 ctgaccgttg tactcgatgg ctgccgcgtc gggctgcagc tccagcacgt cggtgatgcg
    27301 ccggctgagc gggtgcatca gcggactctc ggtgtggctt gcacgccgtt gccctttgcg
    27361 agttcggctt gcccgagcga gtttccgagc cgggtgtaga tcagattctg ttccatcgcc
    27421 gcgcggtatg gcttgtccaa tgactcccag attgctttga cggtgccttg ggtcgccgac
    27481 ggtggtttgg cggcgatcgc cgccgcgatc tcgtgcgcgc gcgcccaca
//
LOCUS       DASRJA010000769         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1529597,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000769 DASRJA010000000
VERSION     DASRJA010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529597"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VFC01_34850"
     CDS             complement(<1..1050)
                     /locus_tag="VFC01_34850"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN84836.1"
                     /translation="MSSRLTAPYRRLFLSVVVALAISACQKKTEPAADAPAAAAGPTA
                     DALEGQVDIVAWPGYVERGDTDKTYDWVSAFEQETACKVNVKTAGTSDEMVSLMTQGG
                     YDLVTASGDASIRLIRGGTVQPVDISRIPSYANVDDRLKEAPWHFVDGKHYGTPYQWG
                     PNVLMYNTTVFKTAPTSWSVVLEPQKLPDGKPNKGRIQAYDGPIHVADAALYLMAKRP
                     ELGIKSPYELNEEQYTAALEVLRKQHPLVHRYWHDANVQVQDFTNEGVVASSSWPFQV
                     NTLVANTQPIASTVPAEGATGWADTTMLATDAKHPNCAYRWMEWSLNAKVQGDLASWF
                     GSVPAVPAACEGNALL"
     gene            complement(1176..2276)
                     /locus_tag="VFC01_34855"
     CDS             complement(1176..2276)
                     /locus_tag="VFC01_34855"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZN84837.1"
                     /translation="MLRNTPSSYGSVAKLLHWSIVVLIITQVVLGVSADDLPNGLRKL
                     ELLAWHKSFGMLILTLALVRLAWRFANPRPVPPADMPRWQRLAAVGTHRLLYALILLQ
                     PLTGWLMSSFKNYPVSFFGLFQWPDLVAPDAGMHELFEEVHEWLARLLVTVAIIHASA
                     ALYHHFGRRDDVLRRMLPFTGAVLLLILTAGSASAATAWRTDAADSQLVFRAMQAGAE
                     FEGRFKRFAPEIRFDPADLAGSRFLVEIDTRSADTQDGDRDAALASGDFFATDRWPSA
                     RFETTAFAAKGAGKFEARGRLTIRDVTRDVLVPFTFTPAADGQHATLAGGTTIRRLDF
                     GVGQGEWTDTTWVGNEVKIRFELRLASDHSAR"
     gene            2266..>2748
                     /locus_tag="VFC01_34860"
     CDS             2266..>2748
                     /locus_tag="VFC01_34860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84838.1"
                     /translation="MRSIREGSLEYGDHTILTQPAGRGWRGRPKGPGPNRWPALLFAA
                     AACAALAGCATKRPEPPAPGPATSAPRAAVPAGAREFEVDPEASVVTMLVRRAGKLSG
                     FGHVHVVTSDGETGRVWFGAAPDLSGFEVRVPVDAFAVDDPAARAAAGPEFAAKVPED
                     A"
BASE COUNT          435 a          908 c          953 g          452 t
ORIGIN      
        1 gaggagcgca ttgccctcac aggccgccgg caccgcgggc accgatccga accaggacgc
       61 gagatcgccc tgcaccttgg cattgagcga ccactccatc caccggtatg cgcagttcgg
      121 gtgcttggca tccgtggcga gcatggtcgt gtccgcccat ccggtcgcgc cttcggccgg
      181 aaccgtgctt gcgatcggct gcgtgttggc caccagcgta ttgacctgga agggccagga
      241 gctcgaggcg accacacctt cgttggtgaa gtcctgcacc tggacgttgg cgtcgtgcca
      301 gtagcgatgc acgaggggat gctgcttgcg cagcacttcg agcgccgcgg tgtactgctc
      361 ttcgttgagc tcgtagggac tcttgatgcc gagctccggc cgcttggcca tgagatatag
      421 cgccgcatcc gcgacgtgta tcgggccgtc gtacgcctgg atgcgtccct tgttcggctt
      481 gccgtcgggc aacttctgcg gctccagcac gaccgaccac gaggtcggcg ctgtcttgaa
      541 cactgtcgtg ttgtacatca gcacgttcgg accccactgg tagggcgtgc cgtagtgctt
      601 gccgtcgacg aagtgccagg gcgcctcctt gaggcggtcg tccacgttcg cgtagctcgg
      661 gatgcggctg atgtcgacgg gctgcacggt gccaccgcgg atcaaccgaa tcgacgcgtc
      721 gccggaggcg gtgacgaggt catatccgcc ctgcgtcatg agcgacacca tctcatccga
      781 ggtgcccgcg gtctttacgt tcaccttgca cgcggtttcc tgctcgaacg ccgacaccca
      841 gtcgtaggtc ttgtcggtat cgccgcgctc gacgtagccc ggccaggcga cgatgtccac
      901 ctggccctcg agtgcatcgg cggtgggacc ggccgcggct gcgggtgcat ccgcggccgg
      961 ttcggttttt ttctggcagg ctgagattgc cagcgcgacg acgacggaca agaacaagcg
     1021 ccgatacggc gccgtcaacc gggatgacat gggaccccca gcgttcgtac ggttgtgtcg
     1081 ggagatcgtt gctgcgcatg cagcccgggc attctatgcc cgggacgcgc gagttcctaa
     1141 aggtggagtg aaaccggcct tgctgcagcg caggatcagc gcgcgctgtg gtccgaagca
     1201 agccggagct cgaagcggat cttgacctcg ttgccgaccc aggtcgtgtc ggtccattca
     1261 ccctggccga cgccgaagtc gagcctccgg atggtggtac cgcccgcgag cgtcgcgtgc
     1321 tggccatcgg ccgccggggt gaacgtgaac ggcaccagta cgtcccgcgt cacgtcgcgg
     1381 atcgtgagac ggccgcgggc ctcgaacttg cccgcgccct tggcggcaaa cgccgtcgtc
     1441 tcgaaccgtg cgctcggcca gcggtccgtg gcgaagaagt ccccgctcgc aagcgcggcg
     1501 tcgcgatcgc cgtcctgcgt gtcggccgag cgagtgtcga tctcgaccag gaaacgactg
     1561 ccggccaggt cggctgggtc gaagcggatc tccggggcga agcgcttgaa gcggccctcg
     1621 aattccgcgc cggcctgcat ggcgcggaac accagttggc tgtccgccgc gtccgtacgc
     1681 caggcggtcg ccgccgaagc gctgcccgcg gtcagaatca gcagcaggac ggcgccggta
     1741 aacggcagca tccggcgcag cacgtcgtcc cggcgtccga agtggtgata gagcgcggcg
     1801 gacgcgtgga tgatcgccac ggtcacgagc aggcgcgcaa gccactcgtg cacttcttcg
     1861 aagagctcgt gcatccccgc atcgggcgcc accaggtccg gccactgaaa caggccgaaa
     1921 aagctgacgg ggtagttctt aaaggacgac atcagccaac ccgtcagcgg ctgcagcagg
     1981 atcaacgcat agagcaggcg gtgcgtgcca acggcggcga gccgctgcca gcgcggcatg
     2041 tcggcgggcg gcaccgggcg ggggttcgcg aatcgccacg cgagacgcac cagggcgagc
     2101 gtcaggatca gcatcccgaa tgacttgtgc catgcaagca gctccagctt gcgcagcccg
     2161 ttcggcaggt cgtccgctga gacccctagc accacttgtg taattatcag gaccacgatg
     2221 ctccagtgca ggagcttcgc gaccgagccg taggacgaag gcgtattgcg cagcatccgg
     2281 gagggatcct tggagtacgg cgaccacacg atactaacgc agcccgcagg ccgcggttgg
     2341 cgcggcaggc cgaaagggcc agggccgaat cgatggccgg cgcttctgtt tgccgcggcg
     2401 gcgtgcgccg cgctcgcggg ctgtgcgacc aagcgtcccg agccgccggc tccgggaccc
     2461 gcgaccagcg caccgcgggc cgcggtgccg gccggcgcac gcgagttcga ggtcgacccg
     2521 gaggcaagcg tcgtcaccat gctggtccgg cgtgccggca agctgtccgg ctttggtcac
     2581 gtgcacgtcg tgacctccga cggtgagacg ggccgtgtct ggttcggcgc tgcacccgac
     2641 ctgtcgggct tcgaggtgcg tgttccggtc gacgcgttcg ccgtcgacga cccggcggcg
     2701 cgcgccgctg ccggcccgga gttcgcggcg aaggtgcccg aggacgcg
//
LOCUS       DASRJA010000770        14267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2657694,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000770 DASRJA010000000
VERSION     DASRJA010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 14267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14267
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2657694"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFC01_34865"
     CDS             complement(<1..412)
                     /locus_tag="VFC01_34865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009473020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN84839.1"
                     /translation="MRGRHHECEVLDRLLEAVRGGESGALVLRGEPGVGKSALLEYVA
                     GRASGWRVARAAGVQSEMELAYSGLDQLCGPMLDRIDRLPEPQRDALRTVFGLSGGAA
                     PDRFLVGVAVLGLFSDAADEEPLVCLMDDAQWLDQ"
     gene            complement(692..764)
                     /locus_tag="VFC01_34870"
     tRNA            complement(692..764)
                     /locus_tag="VFC01_34870"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(729..731),aa:Arg,seq:cct)
     gene            complement(912..1733)
                     /locus_tag="VFC01_34875"
     CDS             complement(912..1733)
                     /locus_tag="VFC01_34875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HZN84840.1"
                     /translation="MVKDFRFGVGLHDARSRAKVQDAARRAEELGFDVMHVPDHLGAP
                     APFPALMSAADATRTLRFGTFVLNAGFYKPALLARDVGAMRDLTDGRFELGLGTGYVR
                     EEFEAAELPYPSARQRIDHMQHVTDYMREHVPDVAILIAGNGDRVLTVAAQRADIIGF
                     TGGDRAPGADDPLAERIAFVRNAAPDRFDELELNIAITAMPIDSSGMPDLKIPRRFLP
                     DLTDEQLLRHPGVLSGSIRDMADRIRSYRDTYGVSYIIVQAPHAEVFAKVIAELR"
     gene            1892..2983
                     /locus_tag="VFC01_34880"
     CDS             1892..2983
                     /locus_tag="VFC01_34880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HZN84841.1"
                     /translation="MASHPTFGVEEEFLLADPTTGEPVAVNRAVAAHAAEHGVKLQLE
                     LTSCQIETNSNVVGGSSDLRRQLTRLRRIAADAAEASGAQLLAVALPPTRPHEFPITD
                     TPRYREIADKFGMVAEEGICGAHVHVAVPSREAAIRVSNRLRPWLPLFLALTANSAVY
                     RNTDTRYASWRSVVWARWPSAGPPPHFDSVDEYDGMVRMLRRAGAVLDDGMVYWDVRP
                     SAKFPTIEVRVADVPATVAETVLLATLIRASVMTALDDERCHASTPPLSPHALKAAYW
                     KSARDGLDGQAVDLLESHASMPARKLLQRMVEHIRPALEDVGDYDMVKAELARLDDVG
                     NGATRQRRAWRQRHDVKDVIAELADATLS"
     gene            complement(2980..3693)
                     /locus_tag="VFC01_34885"
     CDS             complement(2980..3693)
                     /locus_tag="VFC01_34885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014817485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZN84842.1"
                     /translation="MTEPNDPDLIALATPYALHAVPEAELAEIERLVAAAPPDVAEAF
                     ADEVRAVRETMAVVSAATAVDPPDQLRDRLLIEVSSDPVRSLGRRIKRWQTIGLAAAA
                     AVVIGLGALGVGLALRPAPTPSTAEQVFAAPDVRTVSGQIPSGGTATVVFSREKNAGV
                     LVMNNVTPPKPGSVYQMWLIDDKGPHSAGMMDAKNVTPSTTAVLSDLGDSQTLAFTVE
                     PPGGSAQPTSPPFAKLSLA"
     gene            complement(3690..4271)
                     /locus_tag="VFC01_34890"
     CDS             complement(3690..4271)
                     /locus_tag="VFC01_34890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN84843.1"
                     /translation="MTILTPVARLPLVTTDLDALLRQVARRDVDAFATFYDRTRARVF
                     GLVTRVLRDPGYSEETTQEIYLQVWRTADSYDPAAGSPLAWLMTLAHRRAVDRVRSEQ
                     AASQRESRYGAANVDPPSDQVADSVILRDERRQVADCLGSLTDAQRECIQLAYYDGLT
                     YAQVSERLSANLATIKSRMRDAIRGLRRCLGIA"
     gene            complement(4268..5053)
                     /locus_tag="VFC01_34895"
     CDS             complement(4268..5053)
                     /locus_tag="VFC01_34895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HZN84844.1"
                     /translation="MNFLVVSAASLAILVVVHGATFLIGRRIGRYNVVDVTWGIGFVA
                     VAAVVTALGTGDLFRRVLLLVLVTVWGGRLAWHMIVKSAGKGEDPRYRDLLRGDFSAA
                     HVLRKVFVVQAAATWFVSLPLQVSAVLGPTPALLRPLLIAGVALWVVGVLFEAVGDHQ
                     LRRFRADPANKGLIMDRGLWAWTRHPNYFGDACVWWGLWLTSIAAWVSLLTVLSPVLM
                     TYFLVYATGAKLTEKYMRNRPGFAEYRSRTSFFIPMTPKSRLP"
     gene            complement(5050..6315)
                     /locus_tag="VFC01_34900"
     CDS             complement(5050..6315)
                     /locus_tag="VFC01_34900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN84845.1"
                     /translation="MTIEIAETGSTAIDSDRWPDVARVPRGGLSAMIADWLIRRAAGG
                     LPLRLAYPDGTVVGAADPTLPTMVIHNPEAFSRRVARYGLIGFGESYMAGEWDCDDPV
                     GLLNEFARSVDDLIPPALQRFRRLAVVRPPRAQHNSRAQARRNIAKHYDLSNDLFAEF
                     LDETMTYSSALFDRLPAAPADLPGAQRRKIDRLLDTAHVGPGSRVLEIGTGWGELCLR
                     AAARGAHVRSITLSAEQQDLARQRVAAAGLSDRVDIDLCDYRDVDGRYDAVLSVEMVE
                     AVGYRFWPTYFQTLDRLIVPGGRVAIQAITMPHDRMLATRNTYTWIQKYIFPGGMLPS
                     TEAIIGITERHTRLRTADMYSLRPHYAETLRLWRHRFVRRRDAVAALGFDEVFHRMWE
                     LYLAYSEAGFRSGYLDVYQWTFAPTGSGR"
     gene            complement(6312..7085)
                     /locus_tag="VFC01_34905"
     CDS             complement(6312..7085)
                     /locus_tag="VFC01_34905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HZN84846.1"
                     /translation="MAGGRTQSRGVGVLTPAIYRTRITHLRRAPVHHYFEHTSYSWYV
                     DIDELPRLPRWLRPFAKFDACDHLDGGPNDSLRQRIDAFLDAHGIDLAGGRITALMQA
                     RVLGYVFNPLTLYWCHDADGVLRHIVAEVHNTYGGRHAYLVPPHSDQPAMVRKKLYVS
                     PFNDVDGYYLVRASLPQSELDVRISLHRENQPAFVATLRGTRRRASIGELVKLQIVAP
                     LAPLMGAIGIRVQGITLWLRRVPVVPRPETTEKERVTQL"
     gene            complement(7043..8326)
                     /locus_tag="VFC01_34910"
     CDS             complement(7043..8326)
                     /locus_tag="VFC01_34910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN84847.1"
                     /translation="MHPYERSVAVIGSGVAGLVAAYALSARDRVTLFEADIRIGGHAH
                     THYVDRGDGYIVGVDSAFLVHNDRTYPTLCRLFAELGIATQETDMSMSVRDDASGLEY
                     AGARGIGGLFPSWSSLARPRYLLMLAEIKRFHAAATRLLQGDTDETLGAFVDRHGFSR
                     YFVDHFMTPLVAAVWSCPPGDALRYPARYLLVFLEHHGMLSVFGSPTWRTVVSGSATY
                     VGAILNRVGEVSAGEPVTSVRRVADGVEITAGATRLFDVAVIATHPDQALLMMAEPTA
                     AERAVLGAIPYCTNHAQLHTDESVLPRHRRARASWNYLVTPDDDSVVVTYDVTRLMRL
                     AGPRRFLVTLGGRERVDPATVLAEMKYSHPMYTPESVAAQQLLPTLDDDRVAFAGAYH
                     GWGFHEDGAASGLRAARRLGAQWPAAARRAEALAC"
     gene            8388..9971
                     /locus_tag="VFC01_34915"
     CDS             8388..9971
                     /locus_tag="VFC01_34915"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZN84848.1"
                     /translation="MPSTFFRGGTIWTGVNGQVTDGLLVVDGAVRALGEAAHQQAQGA
                     EDVDLGGGFLMPSFGDGHAHPLPGGLEAVGPLVRACKSVDEIVAEVKRFAAEHPDDEW
                     IVGASYESSMAPDGLFDARWLDAAVPDRPVVLRAWDYHTVWCNSVALERAGITADTPD
                     PELGEIPRREDGSPLGTLREWGAVDLVQKVVPERDPAVRVGALRTAAEYYLSHGVTWV
                     QDAWVEPADVDTYVDAAQRDALDIRFNLGLYADPRYFTGQLEQFVDARRRVQDVDSPL
                     LTANTVKFFADGVIENETGALLAPYCGSLHNHGMQVWDNLADAARAVDELGFQIHIHA
                     IGDAAARQALDAIEYVVATNGPRDRRPVIAHAQLVDDADLDRFAALGVIPNMQPLWAQ
                     LDPLMVELTIPRLGPDRSEKQYRMRSLDESGAPLAFGSDWPVSSGAPLDGIAVAVSRG
                     GWTPQEILAAERALSAYTTGVAKQAFAEGHWGTITPGASADLVWLGADPRATPPNELA
                     DVRIRATYLSGRPVYSATG"
     gene            9983..11359
                     /locus_tag="VFC01_34920"
     CDS             9983..11359
                     /locus_tag="VFC01_34920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZN84849.1"
                     /translation="MSTASSDTPAATPPPTAPGEGHLRRVLGVPSLVLFGLVYIVPLT
                     VFTTYGIVVQASGGRLAVSYLVTLAAMVFTARSYARMAAAYPVAGSTYAYTQRTFGPP
                     IGFLAGWSLLLDYLLLPMLNYLVIGIYLNAAIPAVPAWVFVLATIVIVTVLNIIGIVS
                     VARANVLIIAMQAVFIVVFLVLAFVTLSHSGTVDLMAPLTGDGTAPGLSPVLAGAAIL
                     CLSFLGFDAVSTLSEEANEPTRSVPRAIMIATITAGVMFVVLSYVSQLVYPSNNFADV
                     DAGSLDVMVTAGGKFLETFFTAAYVAGALGSAITSQASVARILYAMGRDGILPRPVFG
                     HIPPRFTTPTYAILVVSVISLAAIWVDLTKLASIVSFGALVAFSAVNLTVIKHYFVDA
                     GERGGGGFVNNLVLPLIGFGLTVWLWFSLSGYALVFGLCWFAIGFLWLLVVTRAFSRP
                     TPVLAMED"
     gene            complement(11356..12426)
                     /locus_tag="VFC01_34925"
     CDS             complement(11356..12426)
                     /locus_tag="VFC01_34925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HZN84850.1"
                     /translation="MRASHSYFAYGSNLCVRQMARRCPGAVNPRPAMLADHDWLINQR
                     GVATVEPFHGSQVHGVLWQVTDHDLATLDSAEGVPVRYRRDRLTVHTDDGPADAWVYI
                     DHRVDPGPPRPGYLERIVDGAEHHGLPHRWIEFLRRWDPASWPMRIHMSSSAAPKSLS
                     ELLASPGINEDSALRSRFGFLAIHGGGLEQMTDVIAERAADAAGASVYVVRHPDHYAH
                     HLPSARYRAQESERLAEFLDHVEVVVSLHGYGRIGRSTQLLAGGRNRELAEHLARHVD
                     VPGYQVVTDLDAIPRELRGMHPDNPVNRARCGGTQLELTPRVRGISPRSGLPGDDGLT
                     PATSKLVQGLAATARSWQLTSA"
     gene            12747..13178
                     /locus_tag="VFC01_34930"
     CDS             12747..13178
                     /locus_tag="VFC01_34930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84851.1"
                     /translation="MIEPTRSKLSRFKYSAMLLAAVAAAAFPPVGLTAVASAEKVWDI
                     ETYDYCMEQSRAHYQRGEITLQELTEAAKTCCEYNDGVWNAATQDCQAPPGDATGSRQ
                     LPGNIRIPSDIATAPVVTKPPRPIQVPSDIAAAPAVSQASA"
     gene            complement(13195..>14267)
                     /locus_tag="VFC01_34935"
     CDS             complement(13195..>14267)
                     /locus_tag="VFC01_34935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HZN84852.1"
                     /translation="VANAMACLVQYLSAKLGLVTGQSLPEAVGERMRRPSRLAYWLQA
                     ELVAMATDLAEVMGGAIALYLLFDLPLLVGGVITGIVSLLMLVVKDRRGQRMFERVIT
                     GLLLVIAIGFLTSLFVEPPPVDSVVAGLVPKFAGTESVLLAAAMLGATVMPHAVYLHS
                     GLARDRHGHPEAGRQRRWLLKVTRLDVGLAMLIAGAVNLSMVLVAATNLQGREGTDSI
                     EGAHAAVRDTLGPTVALLFAIGLLASGLASSSVGAYAGAMIMQGLLHVSIPMVLRRLV
                     TLIPALLILAIGLDPSRALVLSQVVLSFGIPFALIPLVRLTSNRSLMGEDTNHRVTTA
                     LGWTVATLISLLNVVLIYLTVN"
BASE COUNT         2491 a         4783 c         4676 g         2317 t
ORIGIN      
        1 cctgatcgag ccattgggcg tcatccatca gacacaccag cggctcctcg tcggccgcgt
       61 cggagaacag cccaagcacc gccacaccaa ccaaaaagcg gtcaggagcc gccccaccgg
      121 acaacccgaa gaccgtccgc aatgcatcgc gctgcggttc cggcagacga tcaatccgat
      181 ccagcatcgg gccgcacaac tgatccagcc ccgaatacgc cagctccatc tcggactgca
      241 cacccgcggc gcgagccacc cgccatcccg acgccctccc cgccacgtac tccaacagtg
      301 ctgacttgcc cacccccggc tcaccacgca gcaccaaagc gccgctctca cctccccgaa
      361 cggcctccaa caaccgatca aggacctcgc actcgtggtg ccgcccacgc aacgcctaac
      421 gaacccccca cgccatggcg ccgacaatca gcgccgccga gcagatctgc agtgacagtg
      481 atcggccgcc gcccaagagg ccactggaaa gactgacgac gcctagcacc tgttcatcat
      541 tcctcggacg accgccaatg cggtcgtatg tgcgatcaag agaacacaga tgatgcgcta
      601 cccgacgact gccaccgacc aagcaccgac cagaacaagg caagaccagc gggagaatat
      661 ctgcaaacca tcgctcacct gcgcaaatgc gtgcccccgg caggattcga acctgcgaca
      721 ccggctttag gagagccgtg ctctatcccc tgagctacga gggcgtgacc gctcggagct
      781 taccgaaagc cgttcgtcac gcccgcatag agcgaggccg aagccccgcg aacgttcctt
      841 accgctcgac ttttcggcgg aatttccagc ggtaaggaac gttcggccgc caatgcaaga
      901 tcaaacgtga gtcaccgcag ctcggcgatc accttggcga acacctccgc gtgcggcgcc
      961 tgaacgatga tgtagctgac cccgtaggtg tcccggtaac tgcggatccg gtcggccatg
     1021 tcgcggatcg acccggacag cacgcccggg tgccgcagca attgctcgtc ggtcagatcg
     1081 ggcaggaatc ggcgggggat cttcaggtcc ggcattccgg aactatcgat gggcattgcg
     1141 gtgatcgcga tgttgagctc gagctcgtcg aagcggtcag gcgcggcgtt gcgaacgaac
     1201 gcgattcgtt cggcgagcgg atcgtcggca cctggcgccc ggtcgcctcc ggtaaagccg
     1261 atgatgtccg cccgctgggc ggcgaccgtc aacacccggt cgccgttccc cgcgatcagg
     1321 atggccacat cgggcacgtg ctcgcgcatg tagtcggtga cgtgttgcat gtggtcgatc
     1381 cgctggcgtg cactgggata cggcagctcg gcggcctcga attcctcacg cacataaccc
     1441 gtgccgagcc cgagctcgaa tcgcccatcg gtgagatcgc gcatcgcgcc gacgtctcgg
     1501 gcgagcaggg ccggcttgta gaagcccgcg ttgaggacga acgtcccgaa ccgcaacgtg
     1561 cgcgtggcgt cagccgccga catcagcgcc ggaaatggcg cgggcgcccc gaggtgatcg
     1621 ggcacgtgca tgacgtcgaa gcccaactcc tctgcccggc gggcggcgtc ctgaaccttc
     1681 gcccgagatc tcgcgtcgtg caaacccaca ccgaaccgaa agtccttgac cattccacga
     1741 tcgtaggcag cgcaacgaga tgtttgacgg ccaccaccag cgggcaaacc tcatccgctg
     1801 acttcgaatc ccgaagtcca caacagaagg accaaccgcc ggatccggcc cgtcgtgaga
     1861 tctccgacgg gccggatcaa tgtttcgaac catggccagc catcccacct ttggcgtcga
     1921 ggaagaattc ctcctcgccg acccgacgac cggcgaaccg gtcgccgtga acagagccgt
     1981 ggccgcccac gccgccgagc acggcgtcaa gctgcaactc gagctgacca gctgccagat
     2041 cgagaccaac agcaacgtcg tcggcggcag cagcgacctt cggcggcagc tcacccggct
     2101 gcggcggatc gccgccgatg ccgccgaagc tagcggcgca caactgctcg ccgtcgcgct
     2161 gcccccgacg cggcctcacg aattccccat caccgacacc ccgcgctacc gcgagatcgc
     2221 cgacaaattc ggcatggtcg ccgaggaggg catctgcggc gcccacgtcc acgtcgcggt
     2281 acccagccga gaggccgcca tccgggtgag caaccggttg cgaccatggc ttccgctgtt
     2341 tctcgcgctg accgccaact cggcggtgta tcgcaacacc gacacccgct acgccagctg
     2401 gcgcagcgtc gtgtgggcgc ggtggcccag cgccggtccg ccaccgcatt tcgattccgt
     2461 cgacgagtac gacgggatgg tgcggatgtt gcgccgcgcc ggagcggtgc tcgacgacgg
     2521 catggtctat tgggacgttc gcccgtcggc caagttcccg accatcgagg tgcgcgtcgc
     2581 cgatgtgccg gcgacggtcg ccgaaaccgt gctgctggcc acgttgatcc gggccagcgt
     2641 gatgacggcg ctcgacgacg aacgatgcca cgcgtcgact ccgccgcttt caccgcacgc
     2701 actgaaggcc gcgtattgga agtcggcccg tgacggcctc gacggccagg ccgtcgatct
     2761 gctggaaagc cacgcgtcga tgcccgcgcg gaagttgttg cagcgcatgg tcgaacacat
     2821 ccggcccgcc cttgaagacg tcggcgacta cgacatggtg aaggccgaac tggcacggct
     2881 cgacgacgtg ggcaacggcg caacgcgaca gcggcgcgca tggcgccaac ggcatgatgt
     2941 gaaagacgtc atcgcggaat tggcggacgc gacgctgtcc taagccaggg acagcttggc
     3001 gaacggcggg ctcgttggct gtgctgaacc gcccggaggt tcgacggtga acgccagtgt
     3061 ctgcgagtca ccgagatcgg atagcaccgc agtggtcgat ggcgttacgt tcttggcgtc
     3121 catcatcccg gccgagtgcg gtcccttatc gtcgatcagc cacatctggt agacgcttcc
     3181 aggcttgggc ggcgtgacgt tgttcatcac cagcacaccg gcgttcttct cgcgggagaa
     3241 caccacagtc gccgttcccc cggatggaat ctggccggac acggtccgca cgtcgggggc
     3301 ggcgaacacc tgctcggcgg tcgacggagt gggcgcaggc cgtaacgcca gccctacgcc
     3361 gagcgcaccg aggccgatca ccaccgctgc cgctgcagcc agccctatcg tctgccagcg
     3421 cttgatccgc cggcccagtg atcgcaccgg atcactgctc acttcgatca acaggcggtc
     3481 gcgcaactga tccggtgggt cgaccgccgt ggccgccgac accacggcca tggtttcccg
     3541 cacagcgcgc acttcgtcgg caaacgcttc cgcgacgtcc gggggagcgg cagcgacgag
     3601 tcgctcgatc tcggcgagct cggcctcggg caccgcatgc aatgcgtacg gcgtcgcgag
     3661 ggcgatcaga tcgggatcat tcggctcggt catgcgatcc ccagacatct gcgaaggccg
     3721 cggatggcat cgcgcatccg tgacttgatc gtggccagat ttgccgacaa ccgttctgac
     3781 acttgggcat acgtcaaccc gtcgtagtac gccagctgaa tgcattcgcg ttgcgcgtcg
     3841 gtcagcgagc cgaggcagtc ggctacctgg cggcgttcgt cgcgcaggat caccgaatcg
     3901 gccacctgat ccgacggcgg gtcgacgttg gcggcgccgt agcgcgactc acgctggctc
     3961 gcagcctgct cggagcgcac ccgatcgacg gcgcgccggt gcgccaacgt catcagccac
     4021 gccagcggcg agccggccgc agggtcgtag ctgtccgccg tccgccacac ctgcaggtag
     4081 atctcctgcg tggtctcctc gctgtagccg gggtcgcgca acacacgggt gaccaggccg
     4141 aacaccctgg ctcgggtgcg atcgtagaag gtggcgaacg cgtcgacgtc gcggcgggcc
     4201 acctggcgca acaaggcgtc gaggtcggtg gtcacgagcg gtagcctagc caccggcgtc
     4261 aatatcgtca tggtaatcgt gatttgggcg tcattgggat gaaaaatgat gtgcgcgaac
     4321 gatattcggc gaagcccggc cgattccgca tgtacttctc ggtgagcttc gcaccggtgg
     4381 cgtagacaag gaagtacgtc atcagcacag gtgagagcac cgtgagcagc gagacccacg
     4441 ccgcgatgct ggtcagccac aacccccacc acacgcacgc gtccccaaag tagttggggt
     4501 gcctcgtcca tgcccacagc ccgcgatcca tgatcaggcc cttgttggcg gggtcggccc
     4561 tgaagcggcg caactggtgg tcgccgacgg cctcgaacag cacgccgacg acccacagcg
     4621 ccacccctgc gatcagcagc gggcgcagca atgcgggagt cggacccagc accgccgaaa
     4681 cctgcagcgg cagcgagaca aaccacgttg ccgcggcctg aacgacgaat accttgcgca
     4741 gcacgtgcgc ggcagagaag tcgccgcgca gcaggtcgcg gtagcgcggg tcctcgccct
     4801 tgcccgcgga cttgacgatc atgtgccagg ccaggcgccc accccacacc gtcaccaaga
     4861 ccagcagcag aactcgtcgg aacaggtcac cggtccccaa tgctgtgacg acggccgcga
     4921 ccgcgacgaa gccgatgccc cacgtgacgt cgacgacgtt gtaccggccg atcctgcggc
     4981 cgatcaggaa cgtggcgcca tggaccacca ccaggatggc cagcgacgcg gcggatacga
     5041 cgaggaaatt catcgaccac ttcccgtggg tgcgaaggtc cactggtaga catcgagata
     5101 acccgaccgg aaacccgctt cggagtatgc caggtacagc tcccacatcc ggtggaaaac
     5161 ctcgtcgaaa cccaacgccg ccacggcatc ccggcgtcgg acgaaccgat ggcgccatag
     5221 ccgcagggtc tcggcatagt gcggccgcaa cgagtacatg tccgccgtgc gcaaccgggt
     5281 gtgccgctcg gtgatcccga tgatcgcctc ggtcgacggc agcatcccgc cagggaagat
     5341 gtacttctgg atccaggtgt aggtgttgcg ggtggccagc attcggtcgt gcggcatggt
     5401 gatggcctgg atcgcgaccc ggccgccggg cacgatcagc cggtccaacg tctgaaagta
     5461 cgtgggccag aaccggtaac cgaccgcttc gaccatctcc accgacagca cggcgtcata
     5521 gcgtccgtcc acgtcgcggt agtcgcacag gtcgatgtcg acgcggtccg acaggcctgc
     5581 cgccgccacc cgctgccggg ccaggtcctg ctgctcagcc gacagggtga tcgagcgcac
     5641 gtgtgcgccg cgcgccgcgg cgcgcaggca cagttcgccc cagcccgtgc cgatctccaa
     5701 cacccggctg cccgggccga cgtgtgccgt atccagcagg cggtcgatct tgcgtcgctg
     5761 cgcgccgggc aggtcggccg gagcagcggg caggcggtca aacagcgcgc tcgagtacgt
     5821 catggtttcg tcgaggaact ccgcgaacag gtcgttggac aggtcgtagt gcttggcgat
     5881 gttgcggcgc gcttgcgcgc ggctgttgtg ttgggcccgc ggaggccgca ccaccgcgag
     5941 ccgtcgaaat cgttgtaagg caggcggaat caggtcatcg acggatctag cgaactcgtt
     6001 gagcagaccg acaggatcgt cgcagtccca ctcgcccgcc atgtaagact cgccgaatcc
     6061 gatcaggccg tatcgggcaa ccctgcgcga gaaggcctct gggttgtgga tcaccatcgt
     6121 cggaagcgtc gggtcggcgg cgccgaccac cgtgccgtcc ggataggcca gccgcagcgg
     6181 caacccgccc gccgcacgac ggatcagcca gtcggcgatc atggcgctca acccgccacg
     6241 gggcactcgt gccacgtccg gccagcgatc ggaatcgatt gccgtcgaac cagtttccgc
     6301 gatctcgatc gtcacagctg tgtgaccctt tccttctcgg tggtttcggg gcgcggcacg
     6361 acgggaacgc ggcgcagcca caaagtgatc ccctggactc ggattccgat tgcgcccatc
     6421 agtggcgcca ggggggcgac gatctgtagc ttgaccagct caccgatgct cgcgcgtcgc
     6481 cgggtgccgc gcagagtggc gacgaacgcg ggctgattct cccggtgcag cgagatccgc
     6541 acgtccagtt cggactgcgg cagcgaggcc cgaaccaggt agtagccgtc gacgtcgttg
     6601 aacggtgaca cgtagagctt cttgcgcacc atcgctggct ggtcgctgtg gggcggcacc
     6661 aggtacgcgt gcctcccgcc gtaggtgttg tgtacctcgg cgacaatgtg gcgcaggacg
     6721 ccatcggcat cgtgacacca gtacagcgtc aacgggttga acacgtagcc cagcaccctg
     6781 gcctgcatca gcgcggtgat ccggccgccg gccagatcga tcccgtgcgc gtcgaggaat
     6841 gcgtcgatgc gttgtcgcag tgagtcgttc ggcccaccgt caaggtggtc gcaggcgtcg
     6901 aatttggcga atggccgcaa ccagcgtggc agtcgcggca actcgtcgat gtcgacgtac
     6961 cagctgtagc tggtgtgttc gaagtagtga tgtaccggtg cgcggcgcag atgcgttatc
     7021 cgggtccggt atatggccgg cgtcaacacg ccaacgcctc ggctctgcgt gcggccgccg
     7081 gccattgcgc gccgagacgg cgcgccgctc gtagccccga cgcggcgccg tcctcgtgaa
     7141 acccccagcc gtggtacgcg ccggcaaacg ccactcggtc atcgtctaac gtcgggagaa
     7201 gttgttgcgc agcaaccgat tccggtgtgt acatcgggtg gctgtacttc atctcggcaa
     7261 gcaccgtcgc tgggtcgacg cgctcgcggc caccgagtgt caccaggaat cggcgtgggc
     7321 cggcaagccg catgaggcgg gtgacgtcgt aggtgacgac cacgctgtcg tcgtccggtg
     7381 tgacgaggta gttccatgag gccctggccc ggcgatggcg cggcagcacc gattcgtcgg
     7441 tatgcagctg ggcgtgattg gtgcagtagg ggatcgcgcc cagcactgcc cgttcggcgg
     7501 cggtgggttc ggccatcatc agcagcgcct gatcgggatg tgtggcaatc acggcgacgt
     7561 cgaaaagccg tgtggcgccc gcggttatct caacgccgtc ggcgacccgt cgcaccgacg
     7621 tcactggctc gcctgcgctc acctcgccga cccggttgag aatggccccg acgtaggtgg
     7681 ccgagccgct gaccacggtg cgccacgtcg gcgagccgaa caccgacagc atgccgtggt
     7741 gttccagaaa aacgagcagg tagcgcgccg ggtaacgcaa cgcgtctccc ggcgggcacg
     7801 accacaccgc cgcgacgagc ggcgtcatga agtggtcgac gaagtagcgg gaaaagccat
     7861 gtcggtccac gaacgcgccg agcgtttcgt cggtgtcgcc ttgcagcagc cgcgttgccg
     7921 cggcgtggaa gcgtttgatc tcggccagca tcagcagata ccgcggccgg gctagcgacg
     7981 accacgacgg gaacaggccg ccgatgccgc gcgcccccgc atactccagc ccgctcgcgt
     8041 cgtcgcgcac cgacatcgac atatcggtct cctgtgtggc gatgcccagc tccgcaaaca
     8101 gccggcacag cgtcgggtag gtgcggtcgt tgtgcaccag gaaggccgag tcgacaccaa
     8161 cgatgtagcc gtcgccccga tcgacgtagt gggtgtgcgc gtgcccgccg atgcggatgt
     8221 cggcctcgaa gagggtcacc cggtcgcgtg cagacagcgc gtacgcggcg accaagccgg
     8281 ccacgccact gccgatgacg gcgaccgagc gttcataggg atgcatccat ttcacgtctg
     8341 gtattcggaa ccgcgcggcg cgcggatggg tgcgatacgg tcccggggtg ccttcgacgt
     8401 tcttccgtgg cgggacgatc tggaccggcg tgaatgggca ggtcaccgac gggctgctgg
     8461 ttgtcgacgg agctgtgcgg gcgctcggcg aagcggcaca ccaacaagcc cagggggccg
     8521 aggacgtcga cctcggcggc ggcttcctga tgccgtcgtt cggtgatgga catgcccacc
     8581 cgctgcccgg tggcctcgag gcggtcgggc cgctggtccg ggcatgcaag tcggtcgacg
     8641 agatcgtggc cgaggtcaag agattcgccg cagaacatcc cgacgacgaa tggatcgtcg
     8701 gcgcctccta cgagagcagc atggctcccg acggcctgtt cgacgcgcgt tggctcgacg
     8761 ccgccgttcc tgaccggccg gttgtgctgc gggcctggga ttaccacacc gtgtggtgca
     8821 acagcgtggc actcgagcgg gcagggatta ccgccgatac cccggacccc gaactcggtg
     8881 agattccgcg ccgcgaggac ggctcgccgc tgggcacgct gcgggagtgg ggggccgtcg
     8941 acctcgtgca gaaggtggtg cccgaacgcg atccggcggt gcgtgtcggc gcgctgcgca
     9001 cggccgccga gtactacctg tcgcatggcg tgacctgggt acaggacgcg tgggtggagc
     9061 ctgccgacgt cgacacctac gtcgacgccg cccagcggga cgcgctcgac atccggttca
     9121 acctcggcct ctacgccgac ccgcgttact tcaccggcca actcgagcag ttcgtcgacg
     9181 cgcgtcgccg tgtccaggac gtggactcgc cgctgctgac ggcgaacacc gtcaagttct
     9241 tcgccgacgg ggtcatcgag aacgaaaccg gggcgttatt ggcgccgtac tgcggctcgc
     9301 tgcacaacca cggcatgcag gtgtgggaca accttgccga tgccgcgcgc gccgtcgacg
     9361 agctcgggtt ccagatccac atccacgcga tcggtgatgc cgcggcccgc caagcgctgg
     9421 atgcgatcga gtacgtcgtc gcgaccaacg ggccgcgtga ccggcggccg gtgatcgcgc
     9481 acgcgcaact ggtcgacgat gccgacctgg atcggttcgc cgcgctgggc gtgataccga
     9541 acatgcagcc gctgtgggca caattggatc cgttgatggt cgagctgacg atcccgcggc
     9601 tcggcccgga ccgcagtgag aagcagtacc gcatgcgcag tctcgacgag tcaggtgcgc
     9661 cgctggcgtt cggctcggac tggccggtgt cgtcgggcgc gccgctggac gggatcgccg
     9721 tcgcagtgtc gcggggcggc tggacgccgc aggagatcct cgccgccgag cgggcactga
     9781 gcgcatacac caccggtgtg gcgaaacagg ccttcgccga agggcattgg ggcacaatca
     9841 cacctggcgc aagcgccgac ttggtgtggc tgggtgcaga tccgcgcgcg acgccgccga
     9901 acgagcttgc ggatgtccgc atacgggcca catatctgtc aggaaggccg gtttactcgg
     9961 ccacaggttg aaagggattg ctatgtcgac agcgtcgtcg gatacacccg ctgccacccc
    10021 gccgcccacc gcgccgggcg aaggtcacct gcgccgtgta ctcggcgtgc catcgctagt
    10081 cctgttcggg ctggtgtaca tcgttccgct gacggtgttc acgacgtacg ggatcgttgt
    10141 ccaggccagc ggcggccggc tagcggtgtc gtacctggtg acgttggcgg ccatggtgtt
    10201 caccgctcgg tcgtacgcgc ggatggccgc cgcatacccg gtggcgggtt cgacgtatgc
    10261 gtacacgcag agaacgttcg gcccaccgat cggattcttg gccggctggt cactgctgct
    10321 ggactacctg ctcctgccga tgctgaacta cctcgtcatc ggcatctacc tcaacgccgc
    10381 catccccgcg gtcccggcgt gggtgttcgt cctcgctacg atcgtcatcg tcaccgtgct
    10441 caacatcatt ggcatcgtct cagtggcgcg ggccaacgtt ctcatcatcg cgatgcaggc
    10501 ggtgttcatc gtggtgttcc tcgtgctggc cttcgtgacg ttatcacaca gcggcacagt
    10561 cgatttgatg gctccgctga ccggtgacgg caccgcgcca gggttgagcc cggtgctcgc
    10621 gggggcggcg atcctgtgcc tgtcgttcct cggcttcgac gcggtatcga cgctttccga
    10681 agaggccaac gagccgactc gcagtgtgcc gcgcgcgatc atgatcgcga cgatcacggc
    10741 cggtgtgatg ttcgtcgtgc tctcgtatgt gtcgcagttg gtgtacccct ccaacaactt
    10801 cgccgacgtc gacgcaggct ccttggacgt gatggtcacc gcgggcggca aattcctcga
    10861 gaccttcttc accgccgcgt acgtcgccgg cgcacttggg tcggcgatca cctcgcaggc
    10921 gtcggtcgcc cggatcctgt acgcgatggg ccgcgacggc atcctgccgc ggccggtgtt
    10981 cggtcacatt ccgccgcggt tcaccacccc gacctacgcg atcctggtgg tgtcggtgat
    11041 ttcgctggcc gcgatctggg ttgatctgac caaactggct tcgatcgtga gcttcggtgc
    11101 gctggtagcg ttttcggccg tcaacctcac ggtcatcaag cactactttg ttgatgcggg
    11161 tgaacgtggc ggcggaggtt tcgtcaacaa cctcgtgctg ccgttgatcg gcttcggcct
    11221 gactgtgtgg ttgtggttca gcctgtccgg ttacgccctg gtgttcggat tgtgttggtt
    11281 cgcaatcggg ttcctatggc tgctggtggt gacgcgggcg ttcagtcggc ccacacccgt
    11341 gctcgcgatg gaggactaag ccgacgtcag ttgccacgag cgggcggttg cggcaaggcc
    11401 ctgaaccaac ttcgacgtgg caggggtcag gccgtcgtcg cccggtagcc cgcttcgggg
    11461 actgatgcca cgcacccgcg gggtcagttc gagttgggtg ccgccgcagc gcgcccggtt
    11521 gaccggattg tcgggatgca taccccgcag ctcgcgcggg atcgcgtcca ggtccgtcac
    11581 gacctggtag cccggcacgt cgacgtggcg cgccaggtgt tcggcgagct cgcggttgcg
    11641 gcctccagcc aacagttgcg tgctgcgccc gatgcggccg tagccgtgca gtgagacgac
    11701 aacctcgaca tggtcgagga actcggccag ccgctcggac tcctgtgcgc ggtagcgcgc
    11761 cgacggcagg tggtgcgcat agtgatcggg atggcgcacc acgtagaccg acgcgcctgc
    11821 agcgtcggcg gcccgctcgg cgatgacgtc ggtcatctgc tccagcccgc cgccgtggat
    11881 ggccaggaag ccgaaccgcg agcgcagtgc gctgtcctcg ttgatgccgg gggacgctag
    11941 cagctccgaa agcgatttcg gcgcagccga actcgacatg tggatgcgca tgggccagct
    12001 cgcgggatcc caacgccgta ggaattcgat ccagcggtgt ggcaggccgt ggtgctcggc
    12061 gccgtcgacg atgcgttcca gatagccggg tcgcgggggg ccggggtcga cacggtggtc
    12121 gatgtagacc cacgcatcgg ccgggccgtc gtcggtgtgc acggtcagcc ggtcgcggcg
    12181 atagcggacc ggcacgccct cggcgctgtc cagtgttgcc aggtcgtggt cggtgacctg
    12241 ccagaggacg ccatgcacct gggatccgtg gaacggttcg acggtggcca ccccgcgctg
    12301 gttgatcagc cagtcgtggt cggccagcat cgcgggccgc ggattgaccg cgccggggca
    12361 tcgtcgtgcc atctgccgca cgcacaggtt ggacccgtac gcgaagtagc tatgacttgc
    12421 ccgcatcagc atccattctc gcagatgcgg gacctgggcc tttgcggcaa ccccgccccg
    12481 ggggacggcc ttcatgtcac gattccgttt ggggtcttca ccacctaacg ggcgcccggg
    12541 ggggaatgcc ctggtcgacg acgcgcaatt acacaggtcg cctattcaca agcagatggg
    12601 catgagcgtc gtcgagcaca agccgggctt gcccggtgga tagttaagtc tttcctctca
    12661 gcagacaaat ctggcttctg ggcgattaca gaagcaccgc ttggccatgg agcattttcc
    12721 tcaccagacc acgagaggaa tccatggtga tcgagccaac ccgctccaaa ctgagccgct
    12781 tcaagtactc agcgatgctc ctcgccgccg tggcagctgc cgccttccca cccgtcggac
    12841 tcaccgccgt cgcaagcgcc gaaaaagtat gggacataga gacttacgac tactgcatgg
    12901 aacagtcaag ggcgcattac cagaggggcg agatcacgct ccaggagttg accgaggccg
    12961 cgaaaacttg ttgcgagtac aacgacggag tctggaacgc tgccacgcag gactgccagg
    13021 ccccacccgg tgacgccacg ggctcacggc agcttccggg caacattcgg attccgtccg
    13081 acatcgccac cgctcccgtt gtgactaaac caccgcggcc gatccaggtt ccgtccgaca
    13141 tcgcggctgc accggcggtg agccaagcgt ctgcctgata ccgggcgatt cggatcagtt
    13201 cacggtcaga tagatcagca ccacgttcaa caggctgatc agggtcgcaa ctgtccagcc
    13261 gagggccgtc gtcaccctgt ggttcgtgtc ttcgcccatc aggctgcgat tgctggtcag
    13321 ccgcaccagc gggattagcg cgaacggaat cccgaaggac agcacgacct gggacaacac
    13381 cagtgcgcgg ctggggtcca ggccgatcgc caggatcagc agcgcgggga tcagggtgac
    13441 cagccggcgc aggaccatcg gtatggatac gtgcagcaga ccttgcatga tcatcgcgcc
    13501 ggcataggcg cccacggatg acgatgccaa gccggaggcg agcagaccga ttgcgaacag
    13561 cagtgcgacg gtcgggccga gcgtgtcgcg gacggccgcg tgcgcgccct cgatcgaatc
    13621 ggtgccctcg cggccctgca gattggtggc cgcgacgagc accatcgaca ggttcacggc
    13681 gcctgcgatc agcatcgcca ggcccacatc cagccgtgtc accttgagca accagcggcg
    13741 ctgccggccc gcctccggat gaccgtgccg gtcacgggcc aggcccgagt gcaaatacac
    13801 cgcgtgcggc atcacggtcg cgccgagcat cgctgcggcc agcagcacgc tctcggtgcc
    13861 ggcgaatttg ggcacgaggc ccgcaaccac cgagtcgacc ggcgggggct cgacgaacag
    13921 gctggtcaag aaaccgatcg cgatgaccag cagcaggcca gtgatgacgc gctcgaacat
    13981 gcgctggcct cggcgatcct tgacgacaag catcaacagc gacacgatcc ccgtgatcac
    14041 gccgccgacg agcagcggca ggtcgaagag caggtagagc gcgatggcgc cgcccatcac
    14101 ctcggcgaga tccgttgcca ttgctaccaa ttcggcctgc agccagtagg ccagccggct
    14161 ggggcgtcgc atccgttcgc ctactgcttc cggcagcgac tgcccggtca ccagacccag
    14221 cttcgctgac aggtactgca ccagacacgc catggcgttg gccacga
//
LOCUS       DASRJA010000771         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_4196708,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000771 DASRJA010000000
VERSION     DASRJA010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4196708"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFC01_34940"
     CDS             <1..266
                     /locus_tag="VFC01_34940"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZN84853.1"
                     /translation="PVSTRGEGGQALARGEGDMALQAICEILPYKEIELVGPLPRELA
                     AWIDMSTAVSARSQDPDRALAFIRYITRAESTPVWTAKGLKRF"
     gene            complement(385..1227)
                     /locus_tag="VFC01_34945"
     CDS             complement(385..1227)
                     /locus_tag="VFC01_34945"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN84854.1"
                     /translation="MTEKKVWLITGAGRGLGVDIAKAALRAGHAVVATGRDAAKVAAA
                     VGDHERLLAIKLDVTRPQDAQTAVDAAVAQFGRIDVLVNNAGNFFAGFFEELSLEQVR
                     NQLDTLLFGPMNVARAALPVMRKQRSGLLITISSTAGITGQMFCTAYAAAKFGIEGWM
                     ESLTPEIALFGIRTMLVEPGFFRTELLTNDSTTYAQPTIDDYAERTKEIVAAWKSMDG
                     KQGGDPAKLADALVKLAALEEPPARFAAGADAVQTFEAKANALLAQAQAHRELSTALA
                     HDNA"
     gene            complement(1293..2453)
                     /locus_tag="VFC01_34950"
     CDS             complement(1293..2453)
                     /locus_tag="VFC01_34950"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN84855.1"
                     /translation="MAQALKDVQVPDTPLVLKAQGSFFVGGDNIEQTRGELGDLGPGG
                     HIAVNQMYVRYMVPQGGEGKVPVVMVHGATLTGKSWETTPDGRMGWDEYFVRKGHPVY
                     VPDQVGRGRSGFNQAVFNNVRAGSTPPPNQPLWLRFSDESVWPNFRFGLTPGQPFSDS
                     QFPVTAVDELAKQAVPDVSFGGLPTPNPTLKALADLAGQLNGAVLMGHSQSGSFPLES
                     ALLNPGAAKGLVLVEPGSCPARYTADQIATLAKVPMLVVFGDHRDTPTGLPTLPTWQA
                     RFEACQALIGRLKNAGLQAVAPKFVQLTNDVVFDDLWRRSDLSVRDRSLVTIAALAAM
                     GDDDQLDLYLRRAIESGLTRAQITEALTHLGFYAGWPKATKAIMAITRSLAK"
     gene            complement(2686..>2935)
                     /locus_tag="VFC01_34955"
     CDS             complement(2686..>2935)
                     /locus_tag="VFC01_34955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84856.1"
                     /translation="ADFPWMGIWDENHSRTAPPWNGRTLTRGLEFGVSPMPETRRQMI
                     ERGRLFDTPGFRWIGARCRIEVDYRAVLLGAERIPESL"
BASE COUNT          509 a          931 c          964 g          531 t
ORIGIN      
        1 gcccggtcag cacgcgcggc gagggaggac aggcgctggc ccgcggcgaa ggcgacatgg
       61 cgctgcaggc gatctgcgaa atccttccat acaaggaaat cgaactggtc ggtccgctgc
      121 cgcgggagct cgccgcgtgg atcgacatgt cgacggcggt gtcggccaga tcccaggatc
      181 ccgaccgtgc gctcgccttt atccgctaca tcacccgtgc cgagtcgacg cctgtgtgga
      241 ccgcgaaggg gctcaaacgt ttttgacgcg aagcccgtgc gccttgcgga gtcggtcgct
      301 cgatgacgcc ctgttggttg cgggcccccg caaccacatg ttcctttaac gtccagctcc
      361 caccacgctg ccgcatcact gaagtcaggc gttatcgtgc gcgagggcgg tggacagctc
      421 gcggtgggct tgggcctgtg cgagcagcgc gttggccttg gcctcgaacg tctgcacggc
      481 gtcagcgccg gcagcgaagc gggccggtgg ctcctcgagt gcggcgagct tcaccagggc
      541 gtcggcgagc ttggcggggt ctccaccctg cttcccgtcc atgctcttcc aggccgcgac
      601 gatctccttg gttcgctcag cgtagtcgtc gatggtgggc tgggcgtagg tggtcgaatc
      661 gttggtgagc agctcggtgc ggaagaaacc gggctcgacg agcatcgtgc gaattccaaa
      721 cagggcgatc tcgggagtga gcgactccat ccacccctcg atgccgaact ttgcagctgc
      781 atacgccgtg caaaacattt ggccggtgat gcctgcggtc gaggagatgg tgatcagcag
      841 gccggagcgt tgcttacgca tgactgggag ggcagcccgt gcgacgttca ttgggccgaa
      901 cagcaacgtg tcgagttgat tgcggacctg ttcgaggctt aactcctcga agaagccggc
      961 gaagaagttg cccgcgttgt tgaccagaac atcaattcgg ccaaactgcg ctaccgcggc
     1021 atcgaccgcc gtctgcgcat cctgtggacg ggtgacgtcg agtttgattg ccagcagcct
     1081 ttcgtgatcg cccactgcag ccgcgacctt cgcagcatcg cggccggtgg cgacaacggc
     1141 gtggccggcc ctcaaggctg ccttggcgat atcgacaccc aagccacgac cagcgccggt
     1201 gatgagccaa accttcttct cagtcatgga agaatcctcg tctggaccac cggcgaccgc
     1261 cagtcgattg tcacgagccg ttatgtgctc tgctatttag cgagcgacct ggtgatcgcc
     1321 atgatcgcct tggtcgcctt cggccagccc gcgtagaagc cgagatgtgt cagggcctcg
     1381 gtgatctgag cgcgggtcag gccgctttcg atcgcccggc gcaggtacag atcgagctgg
     1441 tcgtcgtcgc ccatcgcggc gagcgcggcg atggtgacca ggctgcgatc ccgcacgctg
     1501 agatcggacc gcctccagag gtcatcgaac accacatcgt tggtgagctg cacgaacttg
     1561 ggtgcgacgg cttgtaagcc ggcattcttg aggcggccaa tcagggcctg acaggcctcg
     1621 aaacgggcct gccatgtggg cagggtcggg aggcctgtcg gggtgtcgcg atgatcaccg
     1681 aacacgacga gcatcggcac cttcgccaag gtggcgatct ggtcggctgt gtaccgggct
     1741 gggcagctgc ccggttcgac gagcacgaga cctttcgccg cgccgggatt gaggagtgcg
     1801 gattcaagag gaaacgatcc ggattgcgaa tgacccatca gcaccgcgcc attcaactgg
     1861 ccggcgagat cggctaatgc tttgagtgtg gggttggggg tggggaggcc gccaaagctg
     1921 acgtcgggca ccgcctgctt cgcgagttcg tcgacagccg tcaccgggaa ctggctgtcg
     1981 gagaacggct gaccaggggt caggccgaag cggaagttgg gccagacgct ctcgtcgctg
     2041 aatcgcagcc agaggggttg gttgggcggc ggcgtcgagc ccgcgcgcac gttgttgaag
     2101 acggcttggt tgaagcccga tcgcccgcgt ccgacctggt cggggacgta caccggatgg
     2161 cccttgcgga cgaagtactc atcccacccc attcgtccgt ccggcgtcgt ctcccaggat
     2221 ttgcccgtca aggtcgcgcc atgaaccatg acgacaggca ccttgccctc gccgccctgc
     2281 ggcaccatgt agcgcacgta catctggttg acggcgatgt gccctccggg accgagatcg
     2341 cccagctcac cgcgcgtctg ttcgatgttg tcgccgccga cgaagaagct gccctgggcc
     2401 ttcagcacta acggcgtgtc agggacctgc acatccttga gagcctgcgc cattacacct
     2461 ccggccgtaa caaggacggc cgcaagagtg gcagcggcca gcttggccgg tcggtaaccc
     2521 gtcgagaaac ggtgactgtc catgtctgct acgctcctca tcacgcgatc agagtactct
     2581 gcaccggcag ccacggctcc agaccgcgac gcactgcacc agtcagaatc ttgctcgcga
     2641 agaaggcgat tctgagggtg cgcgccttcg ctttcgacga acaccttaga gtgactcggg
     2701 aattcgctcc gcgcccagca gcacggcgcg ataatcgacc tcgatgcggc agcgcgcgcc
     2761 aatccatcgg aagcccggcg tgtcgaacag ccggccacgc tcgatcatct gccgccgcgt
     2821 ttccggcatc ggcgacacgc cgaactccag gcctcgtgtc agcgtgcggc cgttccaggg
     2881 cggcgccgtt cgactgtggt tctcgtccca gatgcccatc cacggaaagt cggcg
//
LOCUS       DASRJA010000772         3123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2464420,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000772 DASRJA010000000
VERSION     DASRJA010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2464420"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VFC01_34960"
     CDS             complement(<1..649)
                     /locus_tag="VFC01_34960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZN84857.1"
                     /translation="MALAVLAAGLTVALRRRADPVQAAASGTAGAVSVTTVPAERGDI
                     GVYLDAIGTVTPVYTSTITSQVSGMVTAVHYREAQHVRRGDPLIDIDPRPFQAQVDQA
                     QGILDRDTHILEQSRMDAQRFRDAWARDAIAKQQLDDQEKLVLQNEGTVKNDRGTVAF
                     NRTQLAYCHITAPFEGRVGLRLVDPGNVVQANSPTPLVVLTQMQPMTVIFSIAQDH"
     gene            1015..1623
                     /locus_tag="VFC01_34965"
     CDS             1015..1623
                     /locus_tag="VFC01_34965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84858.1"
                     /translation="MTDKDRQDTQRWHRLVAMGTNGVLVGALLAIGTVTASILAQDTG
                     PPIAGVTGSLGLEGTMDKFYGGANTAIVKTADGVRHLLHLTNRTSVHGGKVAADSFSK
                     LEEGSQVVVHYVVEGDRKTALEVDRVGEGGLPAVEGTVTYVDRRAKRLTVRLADGTAL
                     ALRLTDRAAQDDGKDVDNGSQVIVYYADDSAEWVAHYFRKVR"
     gene            complement(1701..2246)
                     /locus_tag="VFC01_34970"
     CDS             complement(1701..2246)
                     /locus_tag="VFC01_34970"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HZN84859.1"
                     /translation="MVNEFTSDIHRAATWSLVLSVLMMATGALAIALPMVAGFAVTGI
                     VGWLLIFSGALHLALALSGGGATAVVWEIVLGVLYGAIGFYLLANPVAGLESLTLAIA
                     VYLFVEGIVEFVLSFQLRHRTGAGWLLVDGIITLVLSVLIWTTYPSSAAWVVGTLVGI
                     SMLFSGITRFMLSRRIRRLAV"
     gene            complement(2336..2692)
                     /locus_tag="VFC01_34975"
     CDS             complement(2336..2692)
                     /locus_tag="VFC01_34975"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HZN84860.1"
                     /translation="MFRPRFGVGFGVFLGYPFGYPLYDPWDYWAYPVGPLTFVPGAGY
                     GGVSFSISPGDAVITVDATYAGTVDQFNDPSSPLNLPPGQHHVEIQAPGYRTLDFDVT
                     VEAGQVIPYAGDLQHN"
     gene            2699..2965
                     /locus_tag="VFC01_34980"
     CDS             2699..2965
                     /locus_tag="VFC01_34980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84861.1"
                     /translation="MSEPAVKMASIESVVIKPAVAVKCRATGVCGWAGVSVVVHAATA
                     ESSSATMGWATVESTSSTAVESTAPTAMLSGVNRHRGKHQRSNK"
BASE COUNT          626 a          980 c         1006 g          511 t
ORIGIN      
        1 gatggtcctg cgcgatcgag aagatcaccg tcatcggctg catctgcgtc agcacgacga
       61 ggggggtcgg gctgttcgcc tggacgacgt tgccggggtc gacgagacgc agaccgacgc
      121 ggccctcgaa cggcgccgtg atgtgacagt aggcgagctg cgtccggttg aaggcgaccg
      181 tgccgcgatc gttcttgacc gtcccctcgt tctgcagcac gagcttttcc tgatcgtcga
      241 gctgctgctt cgcgatggcg tcgcgcgccc aggcatcgcg aaagcgctgc gcgtccatcc
      301 gggattgctc gaggatgtgc gtgtcgcgat cgagaatgcc ttgtgcctgg tcgacctgcg
      361 cctgaaacgg tcgcggatcg atgtcgatca gcggatcgcc cctgcggaca tgctgcgctt
      421 cgcgatagtg gacggccgtc accatgcccg agacctggct ggtaatcgtg ctggtgtaga
      481 ccggggtgac ggtgccgatg gcgtcgaggt agacgccgat atcaccccgt tcggcgggca
      541 cggtcgtgac gctgacggca cccgctgttc cggacgcagc cgcctgaacc gggtcggcgc
      601 gcctgcgcag cgccacggtg agaccggccg caaggaccgc caatgccatc gcggcggtcc
      661 ccaggcgtcg gtagcctcca cgcttcatgt catcgaatgg aggcaattcg gggtccggtc
      721 gcaggtcacg gaatccgccg aaacccggcc gccggcgttt acacgcacgc gcgccctgaa
      781 cagttctgcg tgtcgatgcg cagcagccag ccggcaaccg ccttcagtag atcctgccag
      841 cccgagttgt tcgggccaga ctcgcttgtc acgacatcaa ggttcgtgcc gagcgcggaa
      901 cgaacattgc gtcacccggg agcagcgacc ctccgggccg cgatcgcaga cacgcgtcgg
      961 gtcaagtcga gaagcgcgta ccgagcgccg ttggcctgag ccgaggagca ggtgatgacc
     1021 gacaaggaca ggcaggacac acagcgatgg catcggctgg tcgcgatggg cacgaacggt
     1081 gtgctcgtcg gcgcgctgtt ggcgatcggg acggtcacgg cctcgatact cgcacaggac
     1141 accggcccgc caatcgccgg cgtgaccggt tccctgggac tcgaaggcac catggacaag
     1201 ttttacggtg gcgccaacac cgcaatcgtg aagactgcag acggcgtgcg ccatctcctg
     1261 cacctgacga accgaacgtc cgttcacggc ggcaaggtgg ccgctgattc gttcagcaag
     1321 ctcgaggagg gcagccaggt cgtcgtgcat tacgtggtcg agggcgatcg aaagacggcc
     1381 ctcgaagtcg accgggtagg tgagggcggc ctgcccgccg tggagggcac ggtgacgtat
     1441 gtcgatcgca gggcaaagcg gttgacagtc cggctcgccg acggcaccgc cctcgcgctg
     1501 cgcctgaccg accgtgccgc gcaagatgac ggcaaggatg tcgacaacgg gagccaggtg
     1561 atcgtgtact acgctgacga cagtgccgaa tgggtggccc actatttcag gaaagtcaga
     1621 tagcacaccg ctggtgttaa gggagaaggg aaaagggacc tgccttctcc cttgactccc
     1681 acctctattt caacaacctg ttagacggcc aggcgacgaa ttcttcgcga gagcatgaag
     1741 cgtgtgatgc cgctgaacag catgctgatg ccgacgagcg tgccaaccac ccacgcggca
     1801 ctcgaaggat aggtcgtcca gatcagcacc gagagcacaa gggtgatgat gccgtcgacc
     1861 agcaaccacc ccgcacccgt ccggtggcgg agctggaacg agaggacgaa ctcgacgatg
     1921 ccctcgacga acaagtacac ggcaatcgcc agcgtcagtg attcaaggcc ggcgaccggg
     1981 ttcgccaaca ggtagaagcc gatcgcgcca tacagcacac caaggacaat ctcccagacg
     2041 actgcggtcg cgccgccacc gctcaacgcc agcgcaagat gcagagcgcc actgaagatc
     2101 aatagccagc cgacgatgcc ggtcaccgca aacccggcaa ccatcggaag ggcgatggcc
     2161 agagccccgg tcgccatcat cagcacgctg agcacgagcg accacgtcgc cgcgcggtgt
     2221 atgtcggagg tgaattcgtt gaccattttc taatccatcc tttcttcacg cgtgacgcag
     2281 aaggctgacg gccgcggccg accgatggtc gccgcggccg tcttcgaggg atcggtcagt
     2341 tgtgctgcag atcacccgcg taaggaatga cctggccggc ctcgaccgtc acatcgaaat
     2401 cgagcgttct gtatccaggc gcttgtatct cgacgtgatg ttgtcccggc ggcaggttca
     2461 gcgggctgct gggatcgttg aattgatcga cggtgcctgc gtaggtggcg tcgacggtga
     2521 tgacagcatc accgggcgag atcgaaaagc tcacaccgcc gtaccccgca ccgggaacga
     2581 aggtcagcgg cccgactgga tacgcccagt agtcccacgg atcgtagagc ggatagccga
     2641 aggggtatcc aaggaacact ccgaagccga ctccgaatcg tggacggaac acgaagccgt
     2701 gtccgaacct gccgtgaaaa tggcctcgat cgaatcggtt gtgatcaaac ccgccgtggc
     2761 cgtgaaatgc agggccaccg gcgtgtgcgg gtgggccggc gtgtccgtgg tcgtgcatgc
     2821 cgccaccgcg gaatcctcct ccgccaccat gggctgggcc accgtggaat ccacctccag
     2881 caccgccgtg gaatccaccg cccccaccgc catgttgagc ggcgtcaacc ggcaccgcgg
     2941 caaacaccag cgtagtaata aatagagcgc tcatcaggac agaatcatgg agtctcataa
     3001 cagctgcctc ccagcagctg aggcgggcca acttttcccg tcatcgagat gatcgcggtg
     3061 aaaattgccc acacttattt ggacggagca tggcggcgga tgcttattcc acttaggtgg
     3121 tac
//
LOCUS       DASRJA010000773        11690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1249539,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000773 DASRJA010000000
VERSION     DASRJA010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11690
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249539"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VFC01_34985"
     CDS             <1..754
                     /locus_tag="VFC01_34985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011857229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HZN84862.1"
                     /translation="LDGALSGRGAELGRAFVASLTMGSGQFCTNPGLVIAVDGPGLDA
                     FVGAAREALAGTPATPMLTRGIASNYVASVDTLSGEADLIVRGESDYSQTSCRAALFA
                     TDAGSFLGSERLQAEVFGASSLLVRCADIAEMRAVAEGIEGQLTATVHADESDYDDAR
                     QLVPLLELKAGRILFDGWPTGVEVGHAMVHGGPYPATSDSRSTSVGSRAIERFLRPVC
                     YQNVPKSLLPSAIADDNPDHLWRRIDGRLTQD"
     gene            786..1715
                     /locus_tag="VFC01_34990"
     CDS             786..1715
                     /locus_tag="VFC01_34990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HZN84863.1"
                     /translation="MHPTFSGVLFFPVTPFTETGEVDLDLLARHVASGVEAGPGGLFI
                     ACGTGEFHALGVSEFGRVVRTAVDVIAGRVPVYAGAGGSVTQAKLFAQAAQGAGADGL
                     LLLPPYLVEVPQAGLIAYTREVAAVTDLPVIVYNRNNARYTEESAVAVSKIPNVIGFK
                     DGTGDLDQVARIVRAVTDSVDKDFLFFNGMPTAETTQQAYRAIGVPLYSSATFAFAPD
                     LALAFYDALETGNDALANALLREFFHPLVRLRDTVPGYAVSLVKAGVAMEGLPAGPVR
                     APLIMPTADDVTQLRAIIAAGRAVLAEALTEAV"
     gene            1718..2983
                     /locus_tag="VFC01_34995"
     CDS             1718..2983
                     /locus_tag="VFC01_34995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase family protein"
                     /protein_id="HZN84864.1"
                     /translation="MAPSRIRITGARITPVAFADPPLLNTVGVHQPYALRAIIQLDTD
                     AGLVGLGETYADETHLARLEAAASAITGLDVFALNQIRAAIEERLRGDSRAIGTAGMI
                     TSASAVDQVFSPFEVACLDVQGHAAGRPVSDLLGGVVREAVPFSAYLFYKWAAHPGGE
                     PDAFGEALDPDGIVAQARRIVDEYGFTAIKLKGGVFPPEEEMAAMEALRAAFPSHPLR
                     LDPNAAWTPQTSVKVASGLTGILEYLEDPTPGLDGMAEVAQQAPMPLATNMCVVAFDQ
                     LAPAVAKQSVRVVLSDHHYWGGLQRSRLLAGICETFGLGLSMHSNSHLGVSLAAMVHL
                     AGATPNLTYACDTHWPWKTEEIVKPGALSFVDGSVAVPTGPGLGIEIDEEALAALHEQ
                     YLRCGIRDRDDTGYMKSIDPGYEAVSPRW"
     gene            3132..4361
                     /locus_tag="VFC01_35000"
     CDS             3132..4361
                     /locus_tag="VFC01_35000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN84865.1"
                     /translation="MPVDTLSPNVFEAGLPTLEYEFAETPAEVYPRIRAAQQQAPIAV
                     GPYGPEILSYELVRTVLRDSRFRIPPGMSLMLQGITSGPLWDKVMSSILCMEGEPHQR
                     LRSLVSKAFTPRASARLHDTIVEVISEQVDRVAAEGRCDIVTDIAQRYPVPIICALLG
                     APPDDWQKFSDWADDIFKAFAFEVEDDLEAVVMQAWGELDDYVDDMVAHRRRNPTQDL
                     LSDLIRAEDDGDRLNAAELRMLAGGLLLAGTDTTRNQLAASVDVLCDHPDQWALLAQR
                     PELAPRAVEETMRHSPIACATLRIVVEDADLAGVFFPAGTMVLVNTFAANRDTAVYEA
                     ADRFDITREAAPAVLTFGGGIHYCLGANLARLELSEALKVLTRRMSNPSRTGPAPWKP
                     MMGLSGPTALPIEFSRA"
     gene            4354..5025
                     /locus_tag="VFC01_35005"
     CDS             4354..5025
                     /locus_tag="VFC01_35005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN84866.1"
                     /translation="MRSHGWSGNAPATDEEAIDRILDAADEIIAERGSAVRIADVART
                     LGVTRQTVYRYFPSAEALLMVSAMRSANGFLDQFADHLSGLTEPAAAIVEGVAFAAET
                     LAGDRQIEHILSGRTRSGSTVMLTSDTAKAFGRSMLHRLDVDWETHGYDEAALEELAE
                     IGLRTLHSILVDPGQPARDGIALRRFVARWLGPAIVYPRLAQAMDALQPAAPGPRRHR
                     KSAAS"
     gene            complement(5030..5665)
                     /locus_tag="VFC01_35010"
     CDS             complement(5030..5665)
                     /locus_tag="VFC01_35010"
                     /inference="COORDINATES: protein motif:HMM:NF036707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84867.1"
                     /translation="MISLPAPRPVLWPLRPAESARVSRTDDNGRRMVTIEHAPLANVT
                     AEMLVWWYGHVPGTMQYAGETYPRYMVWHPLDHVSYEVENNAPPVRVGSKLHIVEALG
                     RDLDSVLDIHVTVEEITDRSAIISRRIAGSSVVRLENEFESSNGAARYVTRMTLGDDT
                     MLGRLLLNRVARTKALPPPKLERWIRHHIEEIGNLEHFLPTLFDARAPVEA"
     gene            5752..6678
                     /locus_tag="VFC01_35015"
     CDS             5752..6678
                     /locus_tag="VFC01_35015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZN84868.1"
                     /translation="MFSLARLSCFVAVAEELHFGRAAERLHMTQPPLSRQIQQLENEL
                     GVQLIDRTTRTVTLTPAGVAFLPDARRILQLAEGAAQTVKRIPEGDLGTVVVGFTAAS
                     AHVVLPRLLGAAREKLPDVKLDLREMVTSVQLEGLMTGELDLGMARPPVKRPGLVSRP
                     LLHEQLIAALPEAHPMATLGRQLTLNDLDGQDVIMYSPVQARYFNELLISTFTIAGAT
                     PRYVQYVTQVHTMLVLVRSGIGIALVPASAATLHPDGVVFRSIGAFRERPVELDAVWR
                     GDSSNPALLRLLRDVLPQREWTTDDLIEEAVG"
     gene            complement(6960..7142)
                     /locus_tag="VFC01_35020"
     CDS             complement(6960..7142)
                     /locus_tag="VFC01_35020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84869.1"
                     /translation="MPASAVATAAAASAVATPAAASAVAAIPAVAAIPAVAAIPAVAA
                     IPAIPAAGDPGRRKDA"
     gene            7409..7660
                     /locus_tag="VFC01_35025"
     CDS             7409..7660
                     /locus_tag="VFC01_35025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84870.1"
                     /translation="MNLKKIAAAVAILGTIGLGTAAPAAAGPLDPLPQDPDCDCGGDW
                     GWDNGWNNGWNNGWYGGWYPGKWVNGCVTGPYGHVSFCW"
     gene            complement(7748..8023)
                     /locus_tag="VFC01_35030"
     CDS             complement(7748..8023)
                     /locus_tag="VFC01_35030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84871.1"
                     /translation="MSSEHVTEQVPLAEIQEGDTIQDPQSGQWFTVTRTADGTTSVTH
                     KDSDGDTTVIEGYRVYYGDERQGEQVDSRCVMDSRSAKGLVTRQVRE"
     gene            complement(8213..9058)
                     /locus_tag="VFC01_35035"
     CDS             complement(8213..9058)
                     /locus_tag="VFC01_35035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HZN84872.1"
                     /translation="MEEHSPPPPGQPGYGPPPGTSTSKGSGLSRHRLRNPLSIVLIAV
                     IMIGVVAAGLLASELYVRHRAESVVAAAIECVVQDNASVSFGATPILLQLATGHYRNI
                     SIHTAGNRIGSARGMKADVRIDDVRLDGDADARGTVGALDAIITWSADGIRQSIQEAI
                     SPLRGLVTGVQANPDDGTIKLQGNLGSVTTKPQVTDGRVALQVVDVTGLGLLLPGEMV
                     QSALDAFTTHLTENFPLDIHADSVQVTDDVVTAHYSARNTPIQRGSQGNHGDNNNQDP
                     CFAHL"
     gene            9140..9472
                     /locus_tag="VFC01_35040"
     CDS             9140..9472
                     /locus_tag="VFC01_35040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005058335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophore-related protein"
                     /protein_id="HZN84873.1"
                     /translation="MLSKAKLVGAAACMALALSVGAGVAHAEPDVSAIVDSTCTYPQV
                     IAALNDQSPETAAELNTSPIASGWLQGLVAAPPDQRAQMVQQVQGFPGIQQYLPVIFQ
                     VANSCNRY"
     gene            complement(9560..10534)
                     /locus_tag="VFC01_35045"
     CDS             complement(9560..10534)
                     /locus_tag="VFC01_35045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HZN84874.1"
                     /translation="MSTNAHTPQNRPVLHVGPLKPSLAKTLQDDYAAYVLPDEPAEFL
                     AEHGDEIRAVVTSGRTGVDAELMAALPNLGAVVNFGVGYDTTDVDAAAARGVLVSNTP
                     DVLTDCVADTAVGLVIDVMRQFPAADRYVRAGRWPVEGNYPLTHQVSNTRVGIIGLGR
                     IGSAIAKRLSAFGCEISYHNRHAVEGSPYTYAGSPVELARGVDVLIVTAAGGAGTQGL
                     VSREVIEALGADGYLVNIARGSVVDEHALVEALVGGRLAGAGLDVFEDEPNVPEALMQ
                     MDNVVVLPHVASGTVETRAAMEELTLRNLDSFLESGQLVTPVPMPVAR"
     gene            10843..>11690
                     /locus_tag="VFC01_35050"
     CDS             10843..>11690
                     /locus_tag="VFC01_35050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009477017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZN84875.1"
                     /translation="MSPAVRTTRRRQVLAAALAGITVAATGCTVANSRHGGYDVNTLR
                     IVLQEEPPTLEPCESSLTSTGVVVRSNITEPLVERDPKSGDLQPLLSTGWRQTAPTEW
                     TFDIRKGVTFSNGAPFTAQDAAFSIDRAVNSDLQCNVDGYVFGDEKLKVAAPNDTTVT
                     VRTEKPDPILPLRISFVEIVPRTTSTTAKVREPIGTGPYTIDDWEYGQKLRLKRNDTY
                     WGKKPAFARAEYQWRSEGSVRAAMVVNDEADIGTLLGPEDGAGELGVAFPNNETTALR
                     ITGAEAP"
BASE COUNT         1976 a         3822 c         3809 g         2083 t
ORIGIN      
        1 cttggacggc gcgctgtctg ggcgcggcgc tgagcttggc cgcgcattcg tcgcgtcgct
       61 gacgatggga tccggccagt tctgcaccaa tcccggcctg gtgatcgccg tggacggccc
      121 cggcctggat gcgtttgttg gtgcagcgcg cgaggccctt gctggaacgc ctgcgacgcc
      181 gatgctgacc cggggcatcg cctcgaacta cgtggccagc gtggacacgc tcagcgggga
      241 ggccgacctg atagtccgcg gcgagtccga ctacagccaa acttcctgcc gtgcagccct
      301 gttcgcgact gacgctggca gtttcctcgg atccgagcgt ctgcaggccg aggtcttcgg
      361 agcgtcgagc ctgctggtcc ggtgcgctga catcgccgaa atgcgtgccg tcgccgaggg
      421 catcgaagga cagctgacag ccaccgtgca cgccgacgaa tccgactatg acgacgcccg
      481 gcagctggtg ccgctgctcg agctcaaggc tggccggatc ctgttcgatg gctggccgac
      541 cggtgtcgag gtcggccacg ccatggtgca cggtggcccc taccccgcaa cgtcggactc
      601 acgcagcacc tcggtcggct cccgcgcgat cgaacgcttc ctgcggcccg tctgctatca
      661 gaacgtgccg aagtcgttgc ttcccagcgc tattgccgac gacaatcccg atcatctgtg
      721 gcggcgcatc gatggccgac tcacacaaga ctgaccgccg aacaccgagc aaggagtccc
      781 caccgatgca ccccacgttt agtggagttc tgttcttccc tgtcacgccg ttcaccgaga
      841 ccggcgaagt cgatctcgac ctgctggccc gccacgtcgc tagcggcgtc gaggctgggc
      901 ctggcggcct cttcatcgcc tgcggcacag gcgaattcca tgcgctcggg gtctccgaat
      961 tcggccgggt ggtacggaca gcggtcgatg tgatcgccgg acgcgtgccc gtctacgccg
     1021 gagccggcgg ctccgtcacc caagccaagc tcttcgcgca ggcggctcag ggcgccggtg
     1081 cggatgggct attgctgctg ccgccgtacc tcgtcgaggt gccgcaagcg gggctgatcg
     1141 cctacacccg cgaggtcgcc gccgtcaccg acctgcccgt catcgtctac aaccgcaaca
     1201 acgcccgcta caccgaagag tcggccgttg cggtgtccaa gattccgaac gtcatcggtt
     1261 tcaaggacgg cacaggcgat ctcgaccagg tcgcgcgaat cgtccgcgcg gtcacggatt
     1321 ctgtcgacaa ggacttcctg ttcttcaacg gcatgccgac cgcggagacg acgcagcagg
     1381 cctaccgcgc gatcggtgtg cccctgtact cttctgcgac gttcgccttc gcccctgacc
     1441 tggcgctggc gttctacgac gcgctggaaa ccggtaacga tgcgctggcc aacgcgctgc
     1501 tgcgggagtt cttccatccg ctcgtgcggc tgcgtgacac ggtgcccggc tatgcggtgt
     1561 cgctggtcaa ggccggcgtc gcgatggagg gcctacccgc agggccggtc cgcgcgccgt
     1621 tgatcatgcc gacggccgat gacgtgaccc aactgcgggc gatcatcgcg gccggacgtg
     1681 ccgtgctggc cgaggcgctg accgaggccg tgtagccgtg gcgcccagcc ggattcgtat
     1741 cacgggcgcc cgcatcacgc cggtcgcgtt cgccgacccg ccgctgctga acaccgtcgg
     1801 ggtgcaccag ccgtatgcgt tgcgcgcgat catccagctg gacacggacg ccggactggt
     1861 cgggctgggc gagacctacg cggacgagac acatctcgcg cggctcgagg ctgcggcgtc
     1921 tgcgatcacg ggtctggacg tgttcgcgct caatcagatt cgggccgcca ttgaggagcg
     1981 gctgcgcggc gattcccgtg ccatcggtac ggccggcatg atcacctcgg caagcgctgt
     2041 cgaccaagtc ttctcgccgt tcgaagtggc gtgtcttgac gtgcaaggcc acgctgccgg
     2101 caggccggtg tccgacctac tgggcggcgt cgtccgggaa gcggtgccgt tcagcgcgta
     2161 tctcttctac aagtgggcgg cgcacccggg tggcgaaccg gacgcgttcg gtgaggcgct
     2221 ggacccggat gggatagtgg cgcaggcgcg ccgcatcgtc gacgaatacg gcttcaccgc
     2281 aatcaaactc aagggtggcg tgtttccgcc cgaggaggag atggccgcaa tggaagcgct
     2341 gcgggcggcg tttccgtcgc acccgttgcg gctggatccg aacgcggcgt ggacgccgca
     2401 gacgtcggtg aaggtggcct cggggctcac gggcattctc gaatacctgg aagatccgac
     2461 gcccggcctg gacggcatgg cggaggtggc gcagcaggcg ccgatgccgc tggcgacgaa
     2521 catgtgcgtg gtcgcgttcg accagctggc gcccgcggtg gcgaaacagt ctgtgcgggt
     2581 ggtgctttcc gaccaccact actggggcgg gttgcagcgg tcgcgcttgc tggcgggcat
     2641 ctgcgagaca ttcggcctcg ggttgtcgat gcactccaat tcgcacctgg gcgtcagcct
     2701 ggccgcgatg gtgcatcttg ccggggcgac accgaacctc acgtacgcgt gcgatacgca
     2761 ctggccgtgg aagaccgagg agatcgtgaa gcctggggcg ctgtcgttcg tcgacgggtc
     2821 ggtggccgtg ccgactggtc cgggtcttgg catcgaaatc gacgaggagg ccttggccgc
     2881 gttgcatgag cagtatctgc gctgcgggat ccgcgaccgc gacgacaccg gatacatgaa
     2941 gagcatcgat cctggctatg aggcggtcag cccgcgctgg tagcgctgtt gccactgacc
     3001 ggacggattg tcatttttgt atggccgtcg cttaggtttg cggtttaccc tgttcacttt
     3061 ggcggctagt ggccatacat tctaggaaga ttgtctaccc ggatcgtgtg gcgcgctatt
     3121 gggaggcatg catgcccgtc gacactctct cccccaacgt gttcgaggca ggattgccga
     3181 cgctggaata cgagttcgcg gagactcccg ccgaggtcta cccacggatt cgggcagcgc
     3241 aacaacaggc accgatcgcg gtcggcccgt acggacccga gatcctgtcg tacgagttag
     3301 tgcgaacggt gctgcgtgat tcgcgatttc ggattccgcc cggaatgtcc ctgatgctcc
     3361 aagggatcac ttcgggtcca ctgtgggaca aggtcatgag cagcattttg tgcatggagg
     3421 gcgagcccca ccagcggctg cgcagcctgg tgtccaaggc gttcaccccc cgagccagcg
     3481 cacgcttgca cgacacgatc gtcgaggtga tcagcgaaca ggtcgatcgt gtcgccgccg
     3541 aaggccgttg cgacatcgtc accgatatcg cacagcgtta cccggttccg atcatctgcg
     3601 cgctgctggg tgcccctcct gacgactggc agaagttctc agactgggcc gatgacatct
     3661 tcaaagcgtt cgcattcgag gtagaagacg acctagaggc tgtcgtcatg caggcctggg
     3721 gcgaactcga cgactacgta gacgacatgg tggctcacag gcggcgcaac cccacacagg
     3781 atctgctctc cgacctcatc cgtgccgaag acgacggaga tcgcctcaac gccgccgaac
     3841 tgcgcatgct cgcgggtggc ttgttgctgg cgggaaccga tacgacgcgc aatcagctgg
     3901 ctgcctccgt cgacgtactc tgcgaccacc cagaccagtg ggcgctgctg gcgcagcggc
     3961 ccgagctggc accgcgcgcg gtggaggaga ccatgcggca ctcgccgatc gcgtgcgcca
     4021 cgcttcgcat cgtggttgag gacgcagacc tggccggcgt cttcttcccg gccggaacga
     4081 tggtgctcgt gaataccttt gcggccaacc gtgatacagc tgtctacgag gcggctgacc
     4141 gcttcgacat cacccgcgaa gccgcacccg ctgtactgac cttcggcggc ggcatccatt
     4201 actgcctcgg agcgaaccta gcccggctgg aactttcaga agcgctaaaa gtgttgacgc
     4261 gccgcatgtc caacccttcc cgcaccggac cggcgccgtg gaaaccgatg atgggattga
     4321 gtggtccgac ggctctgccc atagagttct ctcgtgcgta gccacggctg gtcgggcaac
     4381 gcgcccgcga ccgatgaaga ggcgatcgac aggatccttg acgcagccga cgagatcatc
     4441 gccgaacgag ggtcggcggt gcgcatcgcc gatgtagcgc gcacattggg ggtcaccagg
     4501 cagacggtgt atcgctactt tccaagcgcg gaagcgttgt tgatggtgag cgcgatgcgt
     4561 tcggccaacg ggtttctcga tcagttcgcc gatcacctca gcggactgac agaacccgct
     4621 gcggcgatcg tagaaggagt ggcgttcgcg gccgagaccc tggcaggtga ccgccagatc
     4681 gagcacatcc tcagcgggcg cacgcgcagc ggatcgacgg tcatgttgac ctccgacacc
     4741 gcaaaggcgt tcgggcgttc catgctccat cggctcgacg tcgactggga gacacacggt
     4801 tacgatgagg ccgccctcga ggagctggcg gaaatcggtc tacgcacgct gcattcgata
     4861 ctcgtcgatc cgggccagcc ggcccgggac ggcatcgcgc tgcgccgctt tgtggcccgg
     4921 tggctgggtc ccgcgatcgt ctacccccgt ctcgctcagg caatggacgc cctgcagccc
     4981 gcggcgccag ggccgaggcg ccataggaag tcggcggcat cgtgacacct catgcttcaa
     5041 ccggcgcgcg ggcgtcgaaa agcgttggca ggaaatgttc caagttgccg atctcttcga
     5101 tgtggtggcg gatccaccgc tcgagtttgg gtggcggtag cgcttttgtg cgggcgacgc
     5161 ggttcagcag cagtcggccg agcatcgtgt cgtcacctag cgtcatgcgc gtgacatacc
     5221 tggcggcgcc gttcgacgat tcgaactcgt tctcgagtcg cacgaccgag ctgcccgcta
     5281 tgcgcctgct gatgatggca gagcggtcag tgatctcttc gacggtgacg tggatgtcga
     5341 gcacgctgtc gaggtcgcgg ccgagcgcct ccacgatgtg cagcttgctt cccactctga
     5401 ccggaggggc gttgttctcg acttcgtagc tgacgtggtc gagcggatgc cacaccatgt
     5461 aacgcggata agtctcgccc gcgtactgca tagtcccggg cacatggccg taccaccaca
     5521 ccaacatctc cgcggtgaca ttggccaacg gggcgtgctc gatggtgacc atccgtcggc
     5581 cgttgtcgtc ggtgcgcgaa acgcgggccg attctgccgg tcgcagcggc cacaggacgg
     5641 gacggggggc gggaagactg atcatcagga gcgcgtaacc ggcggtggtg gcatctaaac
     5701 tgatgcacga cgtgcacgcg gatggcgtgc agtgatacga ggagtgcatc agtgttttcg
     5761 ctcgcccggc tgtcctgttt cgtcgccgtc gcggaggaac tgcacttcgg gcgggccgcg
     5821 gagcggctgc acatgaccca gccgccgctg agccggcaga tccagcagct ggagaacgag
     5881 ctcggcgtgc agctcatcga tcgcaccacc cggacggtga cgctgacgcc ggccggggtg
     5941 gcgttcctgc ccgacgcacg gcgcattctg caactcgccg agggtgcggc gcagacggtc
     6001 aagcggattc cagaggggga tctcggcacc gtcgtcgtcg gattcaccgc ggcgtccgcc
     6061 catgtcgtgc ttccacgact gctgggcgcg gcgcgggaga agctgccgga cgtgaagttg
     6121 gacttgcggg agatggtgac gtcggtacaa ctcgaagggc tgatgaccgg cgagctcgat
     6181 ctgggaatgg cccgcccgcc ggtgaagcgg ccgggccttg tgtcgcggcc gctattgcac
     6241 gaacagttga ttgccgcgct cccagaggcg cacccgatgg ccactctggg acggcagttg
     6301 acgctgaacg atctcgacgg ccaggacgtg atcatgtact cgccggtgca ggcgcggtac
     6361 ttcaacgagc tactgatcag cacatttacc atcgcgggcg ccacaccgcg gtatgtgcag
     6421 tacgtgacgc aggtgcacac gatgctggtg ttggtgcggt cgggcatcgg catcgcgctg
     6481 gtgccggcgt cggcggcgac gctgcatcca gacggggtgg tgttccggtc catcggggca
     6541 ttccgcgagc ggccggtgga gttggacgcg gtgtggcggg gagacagctc gaatcccgca
     6601 ttgctgcggc tgctgagaga tgttctgccc cagcgggaat ggacgactga cgacctgatc
     6661 gaggaggcgg tcggctagag tccacgcggt cgcgtaggcg cttgcatatg cggtggttgc
     6721 aaccggcgta atctttgccc atgagcggcg agcttgtcct tctcggcgtt ttgcttttgg
     6781 gcatgccggt ggccattgcc gtggtcatac aaacgcgtcg cagacgacgt gacgaaaccc
     6841 ccgaggaccg acgacggcgc gacaagctcg atcttgaacg cagccgggct tggatgcgaa
     6901 agaaccgcgt cagaggcgaa ttcggctcac gcaggtggag gtccggaggc gctgccagct
     6961 taggcatctt tgcggcggcc ggggtcgcca gcggcgggga tggcggggat ggcggcgacg
     7021 gcggggatgg cggcgacggc ggggatggcg gcgacggcgg ggatggcggc gacggcggag
     7081 gcggctgcgg gggtggcgac ggcggaggcg gctgcggcgg tggcgacggc tgacgcaggc
     7141 aattagccgt cgacggcaac agtcaattct cacggaaaag cacgacgccc tgcgtggtcc
     7201 ttaacctctg ctatctcgtt gtgcaggcgg tcgcgtgggc ggtggcgtac gcgccgggcc
     7261 gttggacctc ccagtcacca acttctcgtc ggggtggtcg tcatatgcga gttccacatg
     7321 accggccgca cgccggagga atggaacgag atgttcgcgc cggaggacga agaatagact
     7381 tggaggacac cgctaagggg gtgtcctgat gaatctcaag aagattgccg ccgccgtcgc
     7441 catcctcggc accatcggat taggaaccgc cgccccggct gccgccggtc cgctcgatcc
     7501 actgccgcaa gacccggatt gcgactgcgg cggcgattgg ggctgggaca acggctggaa
     7561 caacggctgg aacaacggct ggtacggcgg ctggtatccg ggcaagtggg tcaacggctg
     7621 cgtcaccgga ccgtatggtc acgtcagctt ctgctggtaa ctagccgaag atcagccact
     7681 ggagctgcgc ggccttcgcc acagttccgc cagtttgccg tccttgtagt cgcgtgggcg
     7741 ctcgcgatta ctctcgcacc tggcgagtga cgagcccttt agcggaccgg gaatccatga
     7801 cgcaccggga atcgacctgc tcaccttgtc gttcgtcgcc gtagtagacg cgatacccct
     7861 cgatgaccgt cgtgtcgcca tctgagtctt tgtgagtgac actcgttgtg ccgtccgccg
     7921 ttcgggtgac ggtgaaccac tgaccggact ggggatcttg gatcgtgtca ccctcctgga
     7981 tctcggccag cggcacctgc tctgttacat gctccgaact catcgaccct cccggatcgt
     8041 cgcggtggtt ggctccgtcc ggcggcgatc accttcagga ttctcccgtt aacggcaccg
     8101 gtcaatccca gctttaccgc cactgcccgc aaacttcagg tcagcaagtt tgtgaccttt
     8161 gcagtcgcct aggcgctcgc cgccttcgct ccgccgcgcc atgctggccg gcctataggt
     8221 gtgcaaagca cggatcctgg ttgttgttat ccccgtggtt gccctggctg ccccgctgga
     8281 tcggcgtgtt tcgagccgaa taatgcgctg tcacaacgtc atcggtgacc tgcacgctgt
     8341 cggcatggat atcgagcgga aaattctcgg tcagatgggt ggtgaacgcg tctagcgccg
     8401 actgcaccat ctcgccaggc agcaacaaac ccagtccggt gacatcgacc acctgcagcg
     8461 ccactcgtcc gtctgtaacc tgcggcttgg tggtgacact gcccaggttg ccctgcagct
     8521 tgatggtgcc gtcgtccggg ttggcctgga caccggtgac caggccgcgc aacggcgaga
     8581 tcgcttcctg gatgctctgc cttatgccgt cggcagacca ggtgatgatg gcgtccagag
     8641 ctcccacagt gcccctggca tcggcgtcgc catccagccg gacatcgtcg atccgcacgt
     8701 cggccttcat gcctcgggcg ctgccgatcc gattgccggc cgtgtggatc gagatatttc
     8761 tgtagtggcc ggtcgcgagc tgcagcagaa tcggcgtcgc gccgaacgac acactcgcgt
     8821 tgtcttgcac cacgcattcg atggcggcgg ccaccacgct ctcggcgcgg tgacggacgt
     8881 acaactcact ggcgagcaga ccggcggcga ctacgccgat catgatcacc gcgatcagga
     8941 ctatcgacag cgggttgcgc aggcggtggc ggctgagtcc agaacccttc gacgtcgagg
     9001 ttcccggcgg tgggccgtaa ccgggctggc cgggtggcgg cggggaatgc tcttccatcc
     9061 ctttatcgtg gacccgcaag cgcgcctccg ggctcctctt caatctgtcg actagatgta
     9121 tgagtgctag cgtgactcca tgctgtcgaa ggcgaaactg gttggcgccg ctgcctgtat
     9181 ggcattggcg ctgagcgtcg gcgccggagt cgcccacgca gaacccgacg tgagcgcgat
     9241 cgtcgattcc acgtgcacct acccccaggt gatcgcggcg ctcaatgacc aatcgccgga
     9301 aaccgccgcc gagctcaaca ccagcccgat agcgagtggg tggttgcaag ggctcgtcgc
     9361 cgccccgccc gatcagcgag cgcagatggt gcagcaggtg cagggctttc cgggaatcca
     9421 gcaatacctg ccggtgatat tccaagtcgc caacagctgc aacaggtact gacgcggccc
     9481 gtcggcattc tggaggatta ggcctagcca aaacttgata tcacatgcga tatcaagttt
     9541 ttgaggtgcc taatggtgct cagcgcgcca ctggcattgg caccggcgta acgagctgac
     9601 ctgactcaag gaagctgtcg agatttcgca gcgtgagttc ctccatcgcc gcgcgagttt
     9661 ccaccgtgcc actcgccacg tgtggcagca ccacgacgtt gtccatctgc attagcgctt
     9721 ccggcacgtt cggctcgtcc tcgaacacat cgagccccgc gcccgccagt cgcccgccga
     9781 ccaaggcctc caccaatgcg tgctcgtcca ccacgctgcc ccgcgcgatg ttcaccagat
     9841 acccatccgc gcccagcgcc tcgataacct cacggctcac caaaccctgg gtgcccgcac
     9901 ctcccgccgc agtcacgatc agcacgtcca caccacgcgc caactccacc ggtgagcccg
     9961 catacgtgta cggcgaaccc tcgaccgcgt gtcggttgtg gtacgagatc tcacagccaa
    10021 aagcgctgag ccgctttgcg attgcgctgc cgatccgccc caagccgatg attcccaccc
    10081 gcgtgttgct cacctgatgc gtcaacggat agttcccctc gaccggccac cggcccgccc
    10141 gcacgtaccg gtccgccgcc gggaactgcc gcatcacgtc gatcaccaac ccgaccgccg
    10201 tgtccgccac acaatccgtc agcacgtccg gtgtattgct caccagaacc cctcgagccg
    10261 cggccgcgtc tacgtctgtt gtgtcgtaac ccaccccgaa gttcaccacc gcgcccaagt
    10321 tcggcagcgc cgccatcaac tccgcgtcga cacccgtgcg gcccgacgtc accaccgccc
    10381 tgatctcgtc gccgtgctcg gccaagaatt cggccggctc atcgggcagt acgtacgccg
    10441 cgtagtcgtc ctggagcgtc ttcgccaacg acggtttcag cggtccgacg tgcaacacgg
    10501 gacggttctg cggggtgtgg gcattggtgc tcatggtggt cccaccgtac ggcgacctgc
    10561 gaatacccgt ccaaccccga aatagcatgg acttatgccg aacccgcatg gctgcccgat
    10621 cgacagccgc tgctcggtat cggcgccctc cccgccgcgt cgaaaatgca gttcacacat
    10681 ggtttttcgg ccgcaccaag tgtcggcggt agtgcgggtc agaccccgat tcggccacta
    10741 agcccgggtt gacgctgttt cgacggcgtc cttaccgtgt gtctgcgatc acacgcttga
    10801 tctacatacg tggacgccac aaaccgcagc ccgggaggat ccatgagccc cgcagtacgc
    10861 accacgcggc gacgccaagt gctcgccgcc gcccttgcgg gaatcaccgt ggccgccacc
    10921 ggctgcaccg tcgccaactc ccgccacggc ggctacgacg tcaacaccct gcgcatcgtg
    10981 ctgcaagagg aacccccgac gctggagccg tgcgagagct cgctgacgtc gaccggcgtc
    11041 gtcgtccgct ccaacatcac cgaacccctc gtcgagcgcg atcccaagtc cggcgacctt
    11101 cagccgctgt tgtcgacggg ctggcggcag accgcaccca ccgagtggac gttcgacatc
    11161 cgcaaaggcg tcacgttctc caacggcgcg ccgttcaccg cgcaggacgc cgcgttctcc
    11221 atcgaccggg ccgtcaactc cgacttgcag tgcaacgtcg acgggtatgt gttcggcgat
    11281 gagaagctca aggtggccgc cccgaatgac accacagtga cggtccgcac cgagaaaccg
    11341 gatccgattc tgccgctgag gatttcgttc gtcgagatcg tgccgcgcac caccagcacc
    11401 accgcgaagg tgcgcgagcc catcggcacc ggcccatata ccatcgacga ctgggaatac
    11461 gggcagaaac tgcgcctgaa gcgcaacgac acctattggg gcaagaaacc ggcgttcgcc
    11521 cgcgccgaat accagtggcg cagcgaaggc agcgtgcgcg ccgcgatggt cgtcaacgac
    11581 gaagccgaca tcggtacgtt gctcgggccg gaggacggcg ccggcgagct cggcgtggcg
    11641 ttccccaaca acgaaacaac tgcgctgcgc atcacgggcg ccgaagcccc
//
LOCUS       DASRJA010000774         8237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2778043,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000774 DASRJA010000000
VERSION     DASRJA010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2778043"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VFC01_35055"
     CDS             <1..986
                     /locus_tag="VFC01_35055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HZN84876.1"
                     /translation="AKAMEPYRPSSDFRNADGSDAPWTRADLLKAAGRDHVGLIRKLP
                     RLMRETATGVSRVRRRSKERGQHPDLARNFAPPDCFINHVVSSGRRFATAPLALSDVK
                     ETSKHLGVTLNDIVLAMSAGALRRLLLRYDGRADAPLIAGVPVSYNTSRERLVGNEFT
                     YMTPSLPVHIDDPVERVRLTSTATAIAKENNQLLGPTLLPAWLSYLPPTLAPAMFRAQ
                     ARRMESASVMNLVISNVPGPRERGDIEGAIVTEIYSVGPVVTGSGMNITVWSYVDQLA
                     ISVLTDDLTLDDPHEATDAMIDAFTEIRSAAGLSTDLTPVGSAMPLAAAAG"
     gene            complement(990..1817)
                     /locus_tag="VFC01_35060"
     CDS             complement(990..1817)
                     /locus_tag="VFC01_35060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HZN84877.1"
                     /translation="MPTLQLVKDQAGQQIPEIAKWDPELTKRVIGVLRPIAKRYFRSE
                     IKDLGRIPVGGALLVSNHSGGSTTTDLPTFAVDFYDRFGYDRPLYTLSHDILSVGPAK
                     QFLQQLGFIPASRDNAARALASDAAVMVFPGGDYDATRPTLAQNVIDFNGRTGYVRTA
                     IDAGVPIVPVVSIGGQETQFFLTRGTWLAKRIGLIKRLTRSEIVPIAVGFPFGVTLGG
                     VNLPLPSKIVTQVLPAIDITARFGEDPDVAEVDAYVRKVMQKALDRLAKQRRFPVLG"
     gene            2025..2627
                     /locus_tag="VFC01_35065"
     CDS             2025..2627
                     /locus_tag="VFC01_35065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84878.1"
                     /translation="MPQLDDIVDRTHMATRSFIADDKHAATDRAPANPILRYRLNVVA
                     STTEDVVHSAGGWLCDRARAGWDVNVLVPGGGDPQPLTILGATTQELHDDFLSMVRSA
                     SRVEALAVSADLLAADTRLRDEVVRVLKRGLTEVTVWGQQWPAELGRQADSVQHRVSA
                     AARAFKSHALVAADVSADAATATETLFRIETGAFRPLYSV"
     gene            complement(2634..2888)
                     /locus_tag="VFC01_35070"
     CDS             complement(2634..2888)
                     /locus_tag="VFC01_35070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2631 domain-containing protein"
                     /protein_id="HZN84879.1"
                     /translation="MASTEVERHTGVDVEDVPSAEWGWSKENYRLIHIGGLLSAAFLL
                     LMMKGNHVGHIEDGFLIVFAVLIVIVVVRDWWLRRRGWHR"
     gene            complement(2966..4249)
                     /locus_tag="VFC01_35075"
     CDS             complement(2966..4249)
                     /locus_tag="VFC01_35075"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HZN84880.1"
                     /translation="MPTLLWFRRDLRLHDLPPLLDAAVADGDVLACFVLDPRLTASSG
                     PRRLQYLHDSLRDLRERLDGRLLVTRGRPEKRIPKLAKQIGAASVHVSADFSPFGRRR
                     DDAVRDTLGDVRLEEAGSPYLVSPGRVTKADGTPYKVFTPFYDAWRQHGWRPPADSDA
                     TSVSWIDPADVPGGVDIPDAGVELELPAGETAARTQWANFVDSGLDSYADDRDRPDLD
                     ATSRMSAHLKFGTIHPRTMAADLGRGKGAQAYLRELAFRDFYAAVLYEWPDSAWWNWN
                     REFDAITMDDDRTLFEAWKAGRTGFPIVDAGMRQLAQTGFMHNRLRMIVASFLVKDLH
                     LPWQWGARWFLEQLVDGDIANNQHGWQWAAGSGTDAAPYFRVFNPTTQGKKFDPDGGY
                     VRRWVPESDDDDYPDPIVDHAAERKEALRRYSQIG"
     gene            4288..5160
                     /locus_tag="VFC01_35080"
     CDS             4288..5160
                     /locus_tag="VFC01_35080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HZN84881.1"
                     /translation="MEFWSGTPFMKTTEIVAVARMLDDAGYDGVICSDHLIYPRELSS
                     PYPDSPTGRPGWPPETAWPDSWVLIGAMAAVTQRLRFSNAVYVAPARPLIEVAKQVAT
                     ASVLSDGRVSLGVGVGWMREEYELLGQDFDTRGKRLNEMIPALRELWRGGWVSWSGQY
                     YQVPELMIEPHPETPVPILCGGESDAALRRAARLCDGWIGYAYAWDDAAAYVQKLKKL
                     RREYGRENEPFDILLALFEPPTPDLYKRAEDIGITAVMSAPWMAMDLPPGGVERYREP
                     IERFAETIIAKVRG"
     gene            5175..5588
                     /locus_tag="VFC01_35085"
     CDS             5175..5588
                     /locus_tag="VFC01_35085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZN84882.1"
                     /translation="MADSNDDAVRELVECFYSAIDRRDWSTVEALVSPRLVVEVGSSR
                     PLGWDEWRADLEEFTRGFPDGHHVIEEVLVDGSHGISRCRFTGTHTGEFRGLAPTGTK
                     VSIAGIHIDRFQGDMLVAHRGQLDLHGLLEQLKGG"
     gene            5646..6131
                     /locus_tag="VFC01_35090"
     CDS             5646..6131
                     /locus_tag="VFC01_35090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZN84883.1"
                     /translation="MDLQPYVDAVRHELGVAAAAAGQDAEALAERLTAPLESAIRLAL
                     LEALSEAAEQITRDLAPGSVDVRLRGRDPEFTVSSGLEEGADESYTGPVLAPESEDDG
                     GTWRVTLRLPEHLRARVDAAARGDGLSVNGWLVRAVTAALGGPRRPRGDAGKHFSGWV
                     R"
     gene            6161..6964
                     /locus_tag="VFC01_35095"
     CDS             6161..6964
                     /locus_tag="VFC01_35095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZN84884.1"
                     /translation="MPTFDTPQPITATVEISAGSVRLVASDRDDTIVEVRPRDEARSH
                     DVKAAEQVRVDFSNGTLAVTSQRGFSFPRRGAVVVDITLPSGSRLSAAAASANITAEG
                     QYADCKFASASGDLDVGSVVGNLKADTASGGIIVHAINGTATVSTASGDATIGDLDGD
                     VKFRAASGSLTVNRLHGNLNAQTASGDVTVATAINGGVSVQTSSGDAVVGIAEGTAAQ
                     LDLRTRSGEVRNSLTPSDGPTAGDETLVVHARTASGDVVVQRATADAMS"
     gene            complement(6967..7407)
                     /locus_tag="VFC01_35100"
     CDS             complement(6967..7407)
                     /locus_tag="VFC01_35100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZN84885.1"
                     /translation="MSEFRPPRYLKPMNKFMMAVQKLGIPTGPAMVLTVPGRKTGKPR
                     STPMTPFEHDGGLYVVAGYPGADWASNARAAGAGTLARGRKSRRVKIVELKPAESRPV
                     LRAFAVKVPVGVGFAKRAGLVRDGAPDEFADLAGTLTVFRFDPA"
     gene            complement(7420..>8237)
                     /locus_tag="VFC01_35105"
     CDS             complement(7420..>8237)
                     /locus_tag="VFC01_35105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZN84886.1"
                     /translation="TGNVGRPLVTQLAAAGVEVRAVTRQPDTARFPNGVKAVGTAAEG
                     LPGASAVFLNSRALGDDLEPVVRRARREGVTRLVALSAINADDDFSRQPSRFRGDRNK
                     EVEQLAVDSGLEWVSLRPTVFITNFFGMWAPQIQAGDAVNGPYATASTAPIVDRDISA
                     VAAKALLTDELVGRKIPMTGPQAFTNTEMVEIIGSVLGRPLHYQEVPADLVRQRFIGL
                     GFSAEFADAYIAFLEATVEKPALVTHEVEKILGRPAETFADAVSAHRDLFINQ"
BASE COUNT         1388 a         2701 c         2741 g         1407 t
ORIGIN      
        1 tggccaaggc gatggagcct taccggccgt cctctgactt ccgcaatgca gatggttccg
       61 atgcgccgtg gacgcgcgcg gatctgctga aggccgccgg acgcgatcat gtgggtctca
      121 tccgcaaact gccgcggctg atgcgcgaga cggcgaccgg ggtgtcgcgg gtccgtcgcc
      181 ggtccaagga gcgtggccag catcccgatc tggcccggaa tttcgccccg ccggattgct
      241 tcatcaacca cgtggtgagc tcggggcggc gattcgcgac ggcgccgctt gccctttccg
      301 acgtgaagga aaccagtaag cacctcgggg tcacgctgaa cgacatcgtg ctggcgatgt
      361 cggcgggcgc gttgcgccgg ctcctgttgc gctacgacgg ccgggctgac gcaccgctca
      421 tcgcgggtgt gccggtgagt tacaacacct cgcgggaacg gttggtgggc aacgagttca
      481 cctacatgac gccgtcgcta cccgtgcaca tcgacgaccc ggtggagcgg gtccggctga
      541 cgtcgaccgc caccgccatc gcgaaggaga acaatcagct gctcggtccg accctgctgc
      601 ctgcgtggct gtcgtacctg ccccctacgc tcgctccggc gatgttccgc gcgcaggcca
      661 ggcgaatgga gtcggccagc gtgatgaacc tggtcatctc gaatgtccct gggccacgcg
      721 aacgcggcga catcgaaggt gcgatcgtca ctgaaatcta ttccgtcgga ccggtagtga
      781 ccggcagcgg catgaatatc accgtctgga gctacgtcga ccaacttgcc atctcggtgc
      841 tgaccgacga tctcaccctc gacgatccgc atgaggcaac cgacgcgatg atcgacgcct
      901 tcaccgaaat cagaagcgcc gcagggctat ccaccgacct gacacccgtc ggctccgcga
      961 tgccgctggc cgcggcggct ggctgagcgc tagccgagca ccgggaagcg gcgctgcttg
     1021 gccaaccggt ccagcgcctt ctgcatcacc ttgcggacgt aggcgtccac ctcggcgacg
     1081 tcggggtcct cgccgaaccg ggcggtgatg tcgatcgcag gcagtacctg cgtgacgatc
     1141 ttgctgggca gtggcaggtt caccccgcca agcgtcaccc cgaacgggaa acccaccgcg
     1201 atcggcacga tctcgctgcg ggtcaggcgc ttgatgaggc cgatccgctt ggcgagccag
     1261 gtgccgcgcg tgaggaagaa ctgcgtttcc tggccgccga tcgacacgac gggcacgatc
     1321 ggcacgcccg cgtcgatcgc cgtccgcacg tatccggtcc tgccgttgaa gtcgatcacg
     1381 ttctgtgcca gcgtcggccg cgtcgcgtcg tagtcgccgc cgggaaacac catcactgcg
     1441 gcgtccgagg cgagtgcgcg ggcggcgttg tcccggctgg ccggaatgaa cccgagctgc
     1501 tgcaagaact gcttggccgg cccgacggac aggatgtcgt ggctgagcgt gtagagcggc
     1561 ctgtcgtaac cgaaccggtc gtagaagtcc accgcgaacg tcggcaggtc ggtcgtcgtc
     1621 gaaccacccg aatgattgga gaccagcagg gcaccgccca ccggtatgcg gcccagatct
     1681 tttatttcgg atcggaagta tcgcttggcg atcggacgta gcacgccgat gacgcgctta
     1741 gtcagttccg gatcccactt ggcgatctcc ggaatctgtt ggcccgcttg gtctttgaca
     1801 agctgaagtg ttggcaatgc gccccctggt agtgctgtcc agcgcctggc tcgtgagggt
     1861 tggaccttcc cggccggttg cgcgctgtct aactagcttt acattgtggc cctaaaatgt
     1921 cacgccgatg tgaggcatgc catagcgggt ccgatgggtt gatgcgatgg cctttcgaca
     1981 atcggacctt tacagaacct cacagaagtg tcacgctcat tgttgtgccg cagttggacg
     2041 acatcgtgga caggacacac atggcaaccc gatccttcat cgccgacgac aagcacgctg
     2101 cgaccgatcg cgcgcccgca aacccgatcc ttcgctatcg actcaacgtc gtcgcctcca
     2161 ccacggagga tgtcgtgcac tcggccggcg gatggttgtg cgaccgcgcg agggcgggct
     2221 gggatgtcaa cgtgctggtc cctggtggcg gcgatccgca gccgctcacg attctcggcg
     2281 ccacgacaca ggagctgcac gacgattttc tgtcgatggt gagaagcgcg tcgcgggtcg
     2341 aggcgttggc ggtgagtgcc gatctgctgg ccgccgacac ccgcctgcgc gacgaggtgg
     2401 tccgggtgtt gaagcgaggg ctcaccgagg tcactgtgtg gggtcagcag tggcccgccg
     2461 aactcggccg gcaggccgat tccgtccagc atcgggtgag cgcagccgca cgggccttca
     2521 agtcgcacgc acttgtcgcc gccgatgttt cagcggatgc ggcaaccgcg accgagacgc
     2581 ttttcaggat cgagacaggg gccttccgcc cgctgtactc cgtctgaaac ggactacctg
     2641 tgccagccgc ggcggcgcag ccaccagtcg cgcacgacca cgatgacgat caggacggca
     2701 aacacgatca ggaacccgtc ctcgatatgg ccgacgtggt tgcccttcat catcagcagc
     2761 aggaaagcgg ccgagagcag tccgccgatg tggatcagcc tgtagttctc cttcgaccag
     2821 ccccattccg cggagggcac atcttcgaca tcgacgccgg tgtgtcgttc cacctcggtg
     2881 ctggccacgg ctgctcctca cggtcgagct gagatctgcc gacattctgg cataggtcta
     2941 ctacgcaccg ccgtacaccc gacagtcagc cgatctggct gtagcgacgc agcgcctcct
     3001 tgcgttccgc cgcgtgatcg acgatcgggt cgggatagtc gtcgtcgtcc gattcgggca
     3061 cccaccgtcg aacgtagccg ccgtccgggt cgaacttctt gccttgcgtc gtggggttga
     3121 acacccggaa gtacggcgcg gcatcggtgc cgctgccggc ggcccactgc cagccgtgtt
     3181 ggttgttggc gatgtcgccg tcgaccaact gctccagaaa ccaccgcgcg ccccactgcc
     3241 acggcaggtg cagatccttg accagaaacg atgccacgat cattcgcagc cggttgtgca
     3301 tgaagccggt ctgcgcgagc tgacgcatgc cggcatcgac gataggaaag cccgtgcgcc
     3361 cggctttcca cgcctcgaag agcgttcgat cgtcgtccat tgtgatcgcg tcgaattcgc
     3421 gattccagtt ccaccatgcg ctgtcgggcc actcatagag cacggcggcg tagaagtcgc
     3481 gaaaggccaa ctcccgcagg tatgcctggg ctcccttgcc gcggcccaga tcggcggcca
     3541 tcgtgcgcgg gtggatcgtg ccgaacttca ggtgtgccga catccggctg gtggcgtcga
     3601 gatcggggcg gtcgcgatcg tctgcgtagc tgtcgagccc gctgtcgacg aagttcgccc
     3661 attgcgtgcg ggccgccgtc tcacctgccg gcagttccaa ctcaaccccg gcgtctggga
     3721 tgtcgactcc gcccggcacg tcggccggat cgatccatga caccgacgtg gcgtccgagt
     3781 ccgccggcgg ccgccagccg tgctgccgcc acgcgtcata gaagggtgtg aacaccttgt
     3841 acggcgtgcc gtcagccttg gtaacccggc ccggcgacac cagatacggc gaacccgctt
     3901 cctccagccg gacatcgccc agcgtgtccc gcacggcgtc gtcgcgtcgc cggccgaacg
     3961 gtgagaaatc cgccgacacg tgaacagacg ccgcaccaat ctgcttggcc agcttcggga
     4021 ttcgcttttc tggccggccg cgggtgacca acagccgacc gtcgagacgc tcacgcaagt
     4081 cgcgcagcga gtcgtgcagg tactgcagcc tgcgcggccc cgaggacgcg gtgagccggg
     4141 ggtccagcac gaagcaggcg agcacgtcac cgtcggccac ggccgcgtcg agtaacggcg
     4201 gcaggtcgtg caaccgaaga tcgcgccgga accacaacag cgtgggcatc cattgatgct
     4261 gcccgaattt cgcgatagag aaacaggatg gaattttgga gcggcacgcc gttcatgaaa
     4321 accacggaga tcgtcgcggt ggcgcggatg ctcgacgacg ccggctatga cggggtgatc
     4381 tgttccgacc acctcatcta cccgcgtgag ctgtcctcgc cctatcccga ttcgccgacc
     4441 ggcagacccg ggtggccacc cgaaaccgcg tggccggatt cctgggtgct catcggtgcg
     4501 atggccgcgg tgacacagcg gctgcggttc tcgaacgcgg tctacgtcgc acccgcgcgc
     4561 cccttgatcg aagtggccaa gcaggttgcg acggcgtcgg tgctctccga cggccgggtg
     4621 tcgcttggcg tcggggtcgg ctggatgcgc gaggaatacg aacttctggg ccaggacttc
     4681 gacactcgcg ggaagcggct caacgagatg atcccggcgt tgcgcgaact gtggcgcggc
     4741 ggctgggtgt cctggagcgg ccagtattac caggtgcccg agttgatgat cgagccccat
     4801 cccgagacgc cggtgcccat cctgtgcggc ggtgaatccg acgcggcgct gcggcgcgcg
     4861 gcacggctgt gcgacggctg gatcggctac gcctacgcct gggacgacgc ggcggcctac
     4921 gtgcagaagc tcaagaagct gcggcgcgag tacgggcgcg agaacgaacc gttcgacatc
     4981 ctgctggcgc tgttcgaacc gccgacgccg gatctgtaca agcgcgccga ggacatcggc
     5041 atcactgcgg tcatgtccgc gccgtggatg gccatggacc tgccgccggg cggcgtcgag
     5101 cgctatcgcg aaccgatcga gcggttcgct gaaacgatca tcgccaaggt gcgcggataa
     5161 atatcgttgg cttcgtggct gattcgaacg atgacgcggt aagggaactg gtcgagtgct
     5221 tctactcggc gatcgacagg cgggactggt cgacggtcga ggcgctggtg tcaccccggc
     5281 tagtcgtcga ggtcggcagc tcgcgaccgc tcggctggga tgagtggcgg gcggacctgg
     5341 aggagttcac tcgaggattt ccggatggcc accatgtcat cgaggaagtc ctcgtcgacg
     5401 gttcccacgg catatcgcga tgccgattca ccggcaccca caccggggag ttccgcggcc
     5461 tggcgccgac cggcaccaaa gtctcgatcg ccgggatcca tatcgaccgc tttcagggcg
     5521 acatgctcgt cgcccatcgc ggtcagctgg acttgcacgg gctattggag cagctcaagg
     5581 gcggttaaca gcgcgacgtc aaactgacac ttgcagtgac accatagtga tgtcaccatg
     5641 acatcatgga cttgcagccg tacgtcgacg cggtacgcca cgagcttggt gtggcggccg
     5701 ccgcggcggg acaggacgcc gaggcgctcg ccgagcggtt gaccgcgccg ctggaatctg
     5761 cgatccggct ggcgctgctc gaggcgctgt ccgaggcagc cgagcagatc acgcgcgatc
     5821 tcgcgccggg ctccgtcgac gtgcgactgc gtggccgcga tccggagttc acggtgtcgt
     5881 ccggcctaga agagggagca gacgagtcct atacaggtcc cgtcctcgca cccgagtccg
     5941 aggacgacgg cggcacctgg cgcgtgacgc tgcgtctgcc ggaacaccta cgggcccgag
     6001 tcgacgcggc cgcgcgcggc gacgggttgt cggtcaacgg gtggctcgtt cgtgcggtca
     6061 ccgccgcgct cggcggtccc cgacgaccac gcggcgatgc aggcaagcac ttcagcggct
     6121 gggtccgcta atccgcaaca cctacaagga ggaaggaaac atgcccactt tcgatacgcc
     6181 ccaacccatc accgcgacgg tggagatctc ggccggctcc gttcggctgg tcgccagcga
     6241 ccgcgacgac acgatcgtcg aggtgcgacc ccgcgacgaa gcccgcagcc atgacgtgaa
     6301 ggccgccgag caggtgcgcg tcgacttcag caacggcaca ctcgcggtga caagccaacg
     6361 tggcttctcc ttcccgcgca ggggagcggt cgtcgtcgac atcaccctgc cgtcgggctc
     6421 gcggctgtcg gcggctgctg catcggcgaa catcaccgcc gaaggccagt acgccgactg
     6481 caaattcgcc tcggcaagtg gtgacctgga tgtcggctca gtggtcggaa atctcaaggc
     6541 ggataccgca tccggtggca tcatcgtgca tgccatcaac ggcacggcaa cggtgtccac
     6601 agcctccggt gacgcgacga tcggtgacct cgacggcgat gtgaagttcc gtgccgccag
     6661 cggctcgctg acggtcaacc ggcttcatgg caacctcaac gcgcagaccg cttctggcga
     6721 cgtcaccgtc gcgaccgcca tcaacggcgg tgtgtccgtg caaaccagca gtggcgatgc
     6781 cgtggtcggc atcgccgaag gcactgcagc tcaactggat ttgcggacga ggtcgggcga
     6841 ggtccgcaac tcgctgacac cgtcggacgg tcccaccgcg ggcgacgaga cactcgtcgt
     6901 gcatgcgcgg accgcgtccg gtgacgttgt cgtgcagcgc gcaaccgccg acgcgatgtc
     6961 ctgacgtcag gcggggtcga accggaatac ggtgagcgtg ccggccaggt ccgcgaattc
     7021 atcaggggcg ccgtcgcgca cgaggcccgc gcgtttggcg aacccgacac cgaccggcac
     7081 cttgaccgcg aatgcccgca acactgggcg tgattccgcc ggcttcagtt cgacgatctt
     7141 gacccgtcgg gacttgcgtc cgcgagcgag tgtcccggcg ccggccgcgc gtgcgttgga
     7201 cgcccagtcc gctcctggat agccggccac gacgtagagg ccgccgtcgt gctcgaacgg
     7261 cgtcatcggg gtgctgcggg gttttccggt ctttcgaccc ggaaccgtca gcaccattgc
     7321 cggaccggtc gggatgccga gcttctgcac ggccatcatg aatttgttca tcggcttgag
     7381 atatcgtggg gggcggaact ccgacatggc ttgtctccct tactgattga tgaacaggtc
     7441 tcggtgcgcg gacaccgcgt cggcgaacgt ctccgcgggg cggccgagga tcttctccac
     7501 ctcgtgcgtg accaacgccg gcttctcgac cgtcgcctcc agaaacgcga tgtaggcgtc
     7561 ggcgaactcg gcggagaatc cgagcccgat gaagcgctga cgcacaaggt cagccgggac
     7621 ctcctggtag tgcagcgggc gaccgagcac cgatccgatg atctcgacca tctcggtgtt
     7681 ggtgaatgcc tgcggtccgg tcatcggaat cttgcggccg acgagttcgt cggtgagcag
     7741 cgccttcgcg gcgacggcgg agatgtcgcg gtcgacgatc ggtgcggtcg atgcggtcgc
     7801 gtacggtccg ttgaccgcgt cgcccgcctg gatctgcggc gcccacatgc caaagaagtt
     7861 ggtgatgaac accgttggtc gcaggctcac ccactccaac ccggagtcga ccgcaagctg
     7921 ttcgacctcc ttgttgcggt cgccgcggaa acgtgacggc tgccgtgaga agtcgtcgtc
     7981 ggcgttgatg gccgacagcg caaccaggcg cgtgacgcct tcacggcgcg ctcggcgcac
     8041 cacaggttca aggtcgtcgc cgagtgcgcg ggagttgagg aacaccgccg aggcgccggg
     8101 cagaccctcc gccgcggtgc ccaccgcctt gacgccgtta ggaaagcgcg cggtgtccgg
     8161 ctgtcgggtg acggcgcgga cctcgacgcc tgcagctgcg agttgggtga ccagtgggcg
     8221 gccgacgttg ccggttg
//
LOCUS       DASRJA010000775         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3747007,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000775 DASRJA010000000
VERSION     DASRJA010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3747007"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2311
                     /locus_tag="VFC01_35110"
     CDS             <1..>2311
                     /locus_tag="VFC01_35110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84887.1"
                     /translation="GGRIELDVLRTAVAEPPALVQQRLAPALDEGVLVIEPGVHEAVQ
                     FGHDRIREAVLRSMNDEQRRGLQLTIARRLAAIPEMFAVAAEQYLPVIDAVDEPTERS
                     HVVGLLRHAADQASVIGDYALIGTLLAAALSRVDPSETDTLVELRTARHAALYAMGRL
                     DEADEEYRAIEAVCRDPVDLAKAAAIQVRSLTHARRLPEAIALGLDVLAQLGIAVPTT
                     DGMLAELDQRFENLLRWLEATGPDDDLARPELTDPTMLAGSRVIDAIQPAAYFGQEPI
                     LMAWLSMQALRIWREHGPARPLVGAATGIPVAAVALRNNYALPYRALRRLLALSEARG
                     YEPETSQPRFLLALLGWWFEPLEDSVDEARQAKEGWIGGGDLGNAAYSFATTVRALLE
                     CASSLDACVAELDAGLAFVRRIGSVNMLEVLETYQWVIDVLRGERPTATGAPVSITGY
                     SDTPATLLLAHSLQAMAAAIFGDQASLEHHTAEAVPLVSASPTEYPGAVARALRGLAL
                     AGQIRATDGAARDTLLSELDEVTRWLADRAADAPQNFLHLLRLVEAERAWAVGDFQGC
                     VRGFDAALHGAGQRPRPWHRALIAERAARFYLAHGIDHSGYDLLTQARQQYAAWGATA
                     KVAQLDWAYPSLPPHSDTAAAQRDTRSLDDSLGSGSVSTGTIDLLGILSTSQALSSET
                     SIEGLHARVAQVLAAMTGATDVALLVWNQEHRHWLLPTTGRVGGASTGDTGPMSVLRY
                     LERTREPLVVADATSDDRFARDPYFADVDR"
BASE COUNT          381 a          772 c          772 g          386 t
ORIGIN      
        1 tgggcgggcg catcgagctg gatgtgctgc ggacggccgt cgccgagcct cctgcgctgg
       61 tgcaacagcg gctggcaccc gcccttgatg agggtgtcct cgtcatcgag ccgggagtgc
      121 acgaagcggt gcagttcggc cacgaccgca tccgcgaggc ggttctgcgt agcatgaatg
      181 acgagcagcg acgcggtctg caattgacga tcgcgcgacg gctcgccgcg attccggaga
      241 tgtttgccgt cgccgctgag caatacctgc cggtcatcga cgcggtcgac gagccgacgg
      301 aacggtcaca cgtcgtcgga ctgctgcgtc acgccgccga ccaggcctcc gtgatcggcg
      361 attacgccct gataggcacg ctgctggccg cggcgctgag ccgcgtcgac ccgtcggaga
      421 ccgacaccct cgtcgagctg cgtacggccc gccacgccgc gctgtatgcc atgggacgcc
      481 tggacgaggc ggacgaggaa taccgcgcga tcgaggcggt atgccgcgat ccggtcgacc
      541 tggcgaaggc agcggcgatc caggtgcgca gtctgaccca cgcgagacgg ttgccggagg
      601 cgatcgcgct cggcctggac gtgcttgcgc aactgggcat cgccgtcccc acgacggacg
      661 ggatgctcgc cgagctggac cagcggttcg agaacctgtt gcggtggctt gaagccaccg
      721 ggcccgacga tgacttggct cgcccagaac tcaccgatcc cacgatgctg gccggctcgc
      781 gggtgatcga tgcgatccaa ccggcggcct actttgggca ggagcccata ctgatggcct
      841 ggctgagcat gcaggcgctg cggatttggc gtgagcacgg ccccgcccgt cctctggtcg
      901 gcgcggccac cggcatcccc gtggcggctg tcgcattgcg taacaactac gccctcccgt
      961 atcgagcgct acgacgattg ttggcgctaa gtgaggcccg aggctacgaa cccgaaacgt
     1021 cacagccacg tttccttttg gcgctgctgg gatggtggtt cgagccgctc gaagacagcg
     1081 tcgacgaagc tcgccaagcc aaggaagggt ggatcggcgg tggcgacctc ggcaacgcgg
     1141 catacagctt tgccacgact gtgcgcgctt tgctcgagtg cgcgtcgtca ctggacgcct
     1201 gcgtcgccga gctcgacgcg ggtctggctt ttgtgcgacg catcggcagc gtaaacatgc
     1261 tcgaggtact tgagacctac caatgggtga tcgatgtgct gcgcggcgaa cgccccaccg
     1321 caaccggcgc gccggtttcc atcacggggt acagcgacac cccagcaacc cttcttcttg
     1381 cacacagcct tcaagcgatg gccgccgcca tctttggtga tcaagcctcg cttgagcatc
     1441 acaccgcaga agccgtgccc ttggtgtcgg cctcgcccac tgaatacccc ggtgcagtgg
     1501 cccgcgcgct gagaggactg gccttggcgg gacagatccg cgccacggac ggtgccgcgc
     1561 gagacacttt gctgagcgaa ctcgacgagg tgacgcggtg gctggccgat cgggccgccg
     1621 atgcgccgca gaatttcttg cacctgttgc ggttggtgga ggccgagcgg gcatgggcgg
     1681 tcggcgactt ccaagggtgt gtccgcggtt tcgacgcggc tctacacggc gccggccaac
     1741 gcccacgccc gtggcaccgg gctttgatcg ccgaacgcgc ggcccgcttc tatttggccc
     1801 acggcatcga ccacagcgga tacgacctgc tgacccaggc gcggcagcag tatgccgcct
     1861 ggggcgcgac ggccaaagtc gcccagctgg actgggccta cccgagcctg ccgccacatt
     1921 cggacacggc ggccgcgcag cgcgacaccc ggtctcttga tgattccctt ggcagtggca
     1981 gtgtcagcac gggaaccatc gatctgctgg gcatcttgtc gacatcacag gcgctgagct
     2041 cggaaacgag catcgaaggt ttgcatgccc gggtggcgca ggtgctcgcc gccatgaccg
     2101 gcgccaccga tgtagctctg ctggtctgga accaagaaca ccgccactgg ctacttccaa
     2161 cgacaggccg cgtcggtggc gcgtcgacgg gcgacacagg tccgatgtca gtgctgcgct
     2221 acctcgaacg cacacgcgag ccactggtcg tggccgacgc caccagtgat gaccgcttcg
     2281 ctcgcgaccc ttatttcgcc gatgtcgacc g
//
LOCUS       DASRJA010000776         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3224555,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000776 DASRJA010000000
VERSION     DASRJA010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3224555"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VFC01_35115"
     CDS             <1..1080
                     /locus_tag="VFC01_35115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN84888.1"
                     /translation="PNIAEWEADGIIPREVFAKAGAVGLLGFAADERFGGVGARDFRY
                     NQILIESLMDADCGAVGLSFAVHNDICLPYLADLTTREQQDRWLPGFVSGDLIAAIGM
                     TEPGAGSDIAGIRTSAVDAVDHFVVNGAKTFITNGQNADVVITAVRTSPDRHRGLTLL
                     VLERGMPGFERGRNLDKLGLHCQDTSELVFTDVQVPKENVLGEVRRGFAYMMRNLPQE
                     RMQIAVGSLGRARAALRWTVDYVRERTAFGKPIGALQNTRFALADVATEVSVAEAFID
                     RCVIELNAGRLSPADAAKAKLWATEMEFRCLDACQQLFGGYGYMREYPIARSAIDARV
                     TRVYGGTSEIMREIIGRDLNLVRKP"
     gene            1092..>2464
                     /locus_tag="VFC01_35120"
     CDS             1092..>2464
                     /locus_tag="VFC01_35120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-CoA synthetase"
                     /protein_id="HZN84889.1"
                     /translation="MQVRSHSLSDIPRRSARRYPDKVAIIDGDLTLTFAEFDELVDRA
                     GAALRDNGFAPGDRIALLAHNCWQYAVLAFATARAAVVLVPINFMLTAEEIAFILEHS
                     RVRGCIVEHDLITTAEEAMTPSGSVITKVALNADGQQAPYGWHDFAQWLTTTTPAPDL
                     EVADDHLLRLMYTSGTESRPKGVMHSSRSLVSNYVSTIVAGSMSADDVEIHSMPLYHC
                     AQLDNFLATDIYLGATSIIIPRPDPEVILRGIEAHQVTNYFAPPTIWIALLRSPVFDQ
                     VDLSSLRKGYYGASAMPTEVLHEIRERLPNLQLWNFYGQTEMAPLASALGPDDQSKHA
                     GAAGRPAINVETTILDDTDTPVPHGTVGEIAHRGPHLMLGYLDDPVRTAEAFRGGWFH
                     SGDLGYYDEFGLLHVVDRKKDMIKTGGENVASREVEEVLYRHPTVQEVAVFGIPHPVW
                     VEAVVAAV"
BASE COUNT          459 a          784 c          784 g          437 t
ORIGIN      
        1 cccaacatcg ccgagtggga agcagacggc atcattccgc gcgaggtgtt cgcgaaagcc
       61 ggcgcggtcg ggttgctggg tttcgcggcc gacgaacgtt tcggtggcgt cggcgcgcgg
      121 gacttccgct acaaccagat cctgatcgaa tcgctcatgg acgcggactg cggtgcggtg
      181 ggcctgagtt tcgccgtaca caacgacatc tgcctgccct acttggccga tctgaccacg
      241 cgcgaacaac aggaccgatg gttgccgggc ttcgtcagtg gcgatctgat cgcggccatc
      301 ggcatgaccg agccgggagc cggatccgat atcgcgggca tccgcacatc cgccgtcgac
      361 gccgtcgacc acttcgtcgt caacggcgcc aaaaccttta tcaccaacgg gcagaacgcc
      421 gacgtggtca tcacggcggt acggacgtcg cccgatcgcc accgtggatt gacgctgcta
      481 gtgctggagc gcggcatgcc cgggttcgag cgtggccgca acctagacaa gctgggactg
      541 cactgccagg acacttccga gttggtgttc acggacgtgc aggtgcccaa ggagaatgtg
      601 ctgggtgagg tgcgccgcgg attcgcctac atgatgagaa accttccgca ggaacgcatg
      661 cagatcgccg tgggcagcct cgggcgtgcc cgcgcggcgc tgcgctggac ggtcgactac
      721 gtgcgtgagc gcaccgcgtt cggcaagccc atcggcgccc ttcagaacac ccgcttcgcg
      781 ctcgccgatg tggccaccga ggtatcggta gccgaggcgt tcatcgatcg gtgtgtgatc
      841 gagctcaacg cggggcggct ttcgcccgcg gatgccgcca aggccaaact gtgggcgact
      901 gagatggagt tccgctgcct tgacgcgtgc cagcagctgt tcggtggtta cggctacatg
      961 cgcgaatatc cgatcgcccg ttccgcgatc gacgccaggg tcacccgcgt ctacggtggc
     1021 acttcggaga tcatgcggga aatcatcggg cgtgacctga atctcgtacg gaagccgtga
     1081 agtacaccga catgcaggtg cgtagccact cactaagtga catcccgcga cgctccgcac
     1141 gccgataccc cgacaaggtc gcgatcatcg acggcgatct cacgctgacg ttcgccgaat
     1201 ttgacgaact tgtggatcga gcgggggcag cgttgcggga caacggtttt gcaccgggcg
     1261 atcggatcgc gctgctcgct cacaactgct ggcagtacgc cgtgcttgcc ttcgccaccg
     1321 ctcgagccgc ggtcgtgctc gtgccgatca acttcatgct gaccgccgag gagatcgcct
     1381 tcattctcga gcacagccgg gtccgcggct gcatcgtcga acacgatctg atcaccacgg
     1441 cagaggaggc gatgacccct agcgggtcgg tgatcaccaa ggtggcattg aacgccgatg
     1501 ggcaacaggc gccatacggc tggcacgact ttgcgcagtg gctgaccacc acgactcccg
     1561 caccagacct cgaagtcgcc gacgatcact tgctgcggct gatgtacacc agcggcaccg
     1621 aatcacgacc gaagggtgtg atgcactcca gtcgcagtct ggtgtcgaac tacgtcagca
     1681 ccattgtggc cggatcgatg tcggccgacg acgttgagat tcattcgatg ccgctgtacc
     1741 actgcgcaca actggacaac ttcctggcca ccgacatcta cctcggcgcg accagcatca
     1801 tcatcccgcg gcccgatccg gaagtcatcc tgcgcggcat agaagcgcac caggtcacca
     1861 attattttgc gccaccgacg atctggattg cgctactgcg ttcaccggtt ttcgaccagg
     1921 tcgatctgag cagtttgcgc aagggatact acggcgcatc ggcgatgccg accgaggtgc
     1981 ttcacgagat ccgcgagcgg ctgcccaacc tgcagttgtg gaacttctac ggccagacag
     2041 agatggcgcc gttggccagc gcgctgggtc ccgacgacca gagtaagcac gccggcgcgg
     2101 cggggcggcc cgcgatcaac gtcgagacca caatcctcga cgacaccgat acacccgtcc
     2161 cccacgggac tgtcggcgag atcgcgcacc gtggccctca cctgatgttg ggatacctcg
     2221 acgatccggt gcgcactgcg gaggcgttcc gcggcgggtg gtttcattcc ggggaccttg
     2281 gctattacga cgaattcggc ctgctgcacg tcgtcgaccg caagaaggac atgatcaaga
     2341 ccggcggcga gaacgtcgca agccgtgagg tcgaagaggt cttgtatcgg catcccaccg
     2401 tgcaggaagt ggcggtattc ggcatcccgc atccggtgtg ggtcgaggcc gtggtggcgg
     2461 ccgt
//
LOCUS       DASRJA010000777         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_545466,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000777 DASRJA010000000
VERSION     DASRJA010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545466"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..722)
                     /locus_tag="VFC01_35125"
     CDS             complement(180..722)
                     /locus_tag="VFC01_35125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84890.1"
                     /translation="MGQPDPFDFSDFGPPSGGATPPRIGGGSRPPGADFSTGFDPWAN
                     QPKTQPRARRQDAFGQSSDRDAFGQAITTGTLATAGPPLTWFGVALVMAAAGVVLALV
                     SATGGGGVFAPALAGWLLAGPVAIGALAVYTRVDTRRRSESIYSAPRWAARLYWAVLV
                     VCLVGIALGAWQIALWAGRQ"
     gene            complement(778..1461)
                     /locus_tag="VFC01_35130"
     CDS             complement(778..1461)
                     /locus_tag="VFC01_35130"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HZN84891.1"
                     /translation="MRPSGNPFEPQSVDPFGTPYGDASVPPSTTPFGQAARIGAPYGA
                     PPSADYNTFAVLSPIFAVVVPPAGVVLGHLALPQIRRSGERGRNAAIAGLVIGYLMCV
                     MLIAALIWWLTSDSDSGEATASLSPSASYQVPPAPPRPSTVTSVAPPPNVPRIKLDLA
                     TVPIGTCVEIQLRSDEGNDALDLFKVDCAHKEGVYTVTARVPAGYECRSVYVAAPPDR
                     SFALCLNPY"
     gene            complement(1951..2142)
                     /locus_tag="VFC01_35135"
                     /pseudo
     CDS             complement(1951..2142)
                     /locus_tag="VFC01_35135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
     gene            complement(2503..2595)
                     /locus_tag="VFC01_35140"
                     /pseudo
     CDS             complement(2503..2595)
                     /locus_tag="VFC01_35140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
BASE COUNT          470 a          913 c          905 g          400 t
ORIGIN      
        1 ccggctttct gcgaggccag gcaggcgccg aaccgctttg cgcccccgcc ggtcggctcg
       61 gcgtgcgcgg acggaatcga ccagccggcc agcgccgcga tcatcaacgc gacggcgaag
      121 aactgcgcca gcagaccaat ccgcttgagc ggcagcagga ttcgcggtga cacagacatc
      181 tactgcctgc ccgcccacag agcaatctgc cacgcaccca atgcgatgcc caccagacac
      241 acgaccagca cggcccagta caaccttgcc gcccagcgcg gcgccgaata gatcgactcg
      301 ctgcgccggc gggtgtcgac acgggtgtag accgccaatg cgccgatcgc gactggcccc
      361 gccagaagcc agcccgccag cgcgggcgcg aagacgccgc caccgccggt ggcgctgacg
      421 agggccagta ccaccccggc cgcggccatc accagcgcca cgccgaacca cgtcagcggc
      481 ggcccggcgg tcgccaacgt ccccgtcgtg atcgcctgac cgaaggcatc acggtcgctc
      541 gactgaccga aggcgtcctg gcgtcgggca cgcggctggg tcttcggctg attcgcccag
      601 ggatcgaagc cggtggaaaa gtcggcaccc ggcggtcgcg aacctccgcc gatccgcggt
      661 ggggtggcgc ctccagaggg cggcccgaag tcgctgaaat cgaacggatc gggttgaccc
      721 acgctgatca ccccggcctt cgctggtggg tgttcgcttc gaagatgttc atcggcatca
      781 gtacgggttc aggcacaggg cgaatgagcg gtcaggtggc gcggcgacgt agacggaacg
      841 gcattcgtag ccggcgggga cccgcgcggt gacggtgtac accccttcct tgtgcgcgca
      901 gtcgaccttg aacagatcca acgcgtcgtt gccttcgtcg ctccgcagct ggatctcgac
      961 gcaggtgccg atcggcaccg tggcgaggtc gagcttgatc cgcggaacgt tgggcggcgg
     1021 ggccaccgag gtcaccgtcg agggtcgcgg cggtgccggc ggcacctgat aggaggcgct
     1081 cggcgagagt gaggcggtcg cctcgccgga gtccgaatca ctcgtcaacc accagatgag
     1141 ggcggcgatc agcatcacgc acatcagata gccgatcacg agtcccgcga tggcagcgtt
     1201 gcgtccccgc tcgccggagc gccggatctg gggcaaagcc aggtgcccca acaccacccc
     1261 ggccggcggt accaccaccg cgaaaatcgg cgacagcacc gcgaaggtgt tgtagtcggc
     1321 agacggcggc gctccgtagg gcgcaccgat ccgagcggcc tgcccgaacg gagttgtgct
     1381 cggtggcacg gacgcgtctc cgtacggtgt cccgaacgga tccaccgact gcggctcgaa
     1441 cggattaccg ctgggacgca tcggaacacc tcgtcagcgg cctgtgccga ccgtgagctg
     1501 caggccagcg acccgcgagc ccgcgtccgc ggtctacgcg gctatggcca gtcagtgaac
     1561 gcaacctcat catcatcagc ctcggtaatc cgagatgcac gcgaataccg tttcttgcct
     1621 gtcgaacagt gtctgcccag ggcatgcgct ggtcgtcaag atctggtcta cccggaatac
     1681 gccgtcacgg acctcgcagc gcaccggaaa tatgaggatg acgttcgtgt cgggcccttt
     1741 agtggggtcc ggctgcgtct gctggacctc gacgcagtcg cctacccgca gctgctccac
     1801 tctgaccgtc ggccgctgag cagggggtgg ggtgaagacc gtggtacgcg gaggtggcgg
     1861 cgaagcgcgt tgcgtcgtcg tcggcgtcgc cgtcgtctga ccgggcgacg aaggggactt
     1921 gaaggcgtcg gaggtcaaga gccagatcac cagcgcaatc accgccagca cgatgatgac
     1981 gtaggagatc gtgaggccca cgatcgctcg ttcacgaccg cgttgcccgc tccgcttgat
     2041 ctgtgagagt gctacgtggc ccagggccgc gccggcgggg gcgaacacga acgcgaacac
     2101 gatcgacaag gtcgcgaacg tgttgacttc accgcctggc ctacccgatg gcgggaagac
     2161 cggacctgtc gtagggggcg agcctggggt gagcggcggc acgtacccct gggtgctcgg
     2221 cggaccgccg gcggggtccc aaccgaacgg gtcgggctga ccgcccccgt acccgccgtg
     2281 cgtcatctgg ttatccgccc ccgttctgac gtgcctctcg tcaccgattc catcaccatc
     2341 aatggtgcgc tatccccaga acggggggtg cgaaatgcga tcgtgatgca cctgggccta
     2401 agccatcgac agcccgccag tcaggctgaa cgacgcgcga gcgcaagcac atcctcggtc
     2461 gacgggaatc gcgtcgcgtg cttctgcgga aatgtgactc ggctaggccc gcacggtcgg
     2521 ccagaagagg tcgaacaacc gcgcttccca tcccgtcgcg acccgcccgg tgcgggcacg
     2581 ctcggccagt gccgcagaag ttcggcgacg agacacgggc caactgtcgc tgttttagac
     2641 ggaggcatgt cactcgcccc cgggcgagcc tgggcagttt ttccgtcg
//
LOCUS       DASRJA010000778        12119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3789124,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000778 DASRJA010000000
VERSION     DASRJA010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 12119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12119
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3789124"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFC01_35145"
     CDS             complement(<1..381)
                     /locus_tag="VFC01_35145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZN84892.1"
                     /translation="MADEHTDYRLHGVRVVHADELDPNTPQTPGMNRAAAINAARAGA
                     EKLWAGTVIIHPDAKTGPHHHGHLESVIYVVAGRARMRWGDRLEFTAEAGPGDFIYVP
                     PYVPHQEINASTDEVLSCVLVRSDS"
     gene            complement(439..1188)
                     /locus_tag="VFC01_35150"
     CDS             complement(439..1188)
                     /locus_tag="VFC01_35150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZN84893.1"
                     /translation="MTQQVREVGAFGLWVEHFEQNHAVHASTDAAIGFDGVCEIPEPV
                     RQPLIASIRRFQLGESGEGAQLLRKAAKAGDAEYLDAARLFIGEEQQHAALLLRLLAY
                     LGGEQMRRHWSDAVFVRLRRLMGLRTELMVLTVAEVVALWYYGGLATDGPDPVVRAVA
                     GRIVADEHPHVRFQQDRLRIGFARSGALARLLAFAFWWTTAIGATVVVAIDHGPLLDA
                     IGYRRTRFVRDVLADFAKVCATVLYRRDTRL"
     gene            1222..2607
                     /locus_tag="VFC01_35155"
     CDS             1222..2607
                     /locus_tag="VFC01_35155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZN84894.1"
                     /translation="MEARWVLHFDMDAFFASVEQLTRPTLRGRPVLVGGLGGRGVVAG
                     ASYEARVYGARSAMPMHQARRLVGAPAVVLPPRGVVYGVASRRVFETVRTLVPVLEQL
                     SFDEAFGEPAELAGADADAVDAFCQQLKARIREETGLVASVGAGSGKQIAKIASDLAK
                     PDGIRVVRRDEESVLLHGLPVRRLWGIGPVAEEKLHRLGIETVGALAALTDAEVANIL
                     GATVGPALHRLAQGIDDRPVAENAPAKQISAESTFPEDLTTLDQLREAVGPIGEHAHT
                     RLEKDGRGARTVTVKLKKSDMSILTRSATLPYATTDVSTLIAMARRLLLDPIEIGPIR
                     LVGVGFSGLSDVRQESLFPDLELVADDVPNTSLPPTVTDLAAMPPAWRVGDDVVHTEF
                     GHGWIQGAGHGVMTVRFETRSSGPGPVHTFPDDTPGLERANPVDSLDWADFLSELAAR
                     SAPAGDDVLDR"
     gene            complement(2572..3489)
                     /locus_tag="VFC01_35160"
     CDS             complement(2572..3489)
                     /locus_tag="VFC01_35160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HZN84895.1"
                     /translation="MGRLVVVTTGGTIATSSDHDGVKRPTRSGAELTDGLDVEVIDLM
                     AVDSSQLTPAHWDQIRSAIPSDADGVVITHGTDTMEETALWLELTYDGALPVVITGAL
                     HSADAPDADGPGNMRDALTLAGSADARGLGVLVTLAGVVWQPLGLQKAATGDLRGFTG
                     EVLGRVGDGCFTAQRSKERPFLGQLSAADAPRVDTIAVYPGADAVAMAACVAAGARGI
                     VLEAVGSGNAGAAVIDGVRRHCRDGVAVAVSTRVPGGRVSAGYGPGRELVDAGAVVVP
                     RLPAAQARVLVMAALAAGSPVEDVVARWG"
     gene            3532..4122
                     /gene="lspA"
                     /locus_tag="VFC01_35165"
     CDS             3532..4122
                     /gene="lspA"
                     /locus_tag="VFC01_35165"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZN84896.1"
                     /translation="MTDEPTGSAEPVIDGDGISGSAGGEAPQPAPNRRLRLLLTVAAV
                     VLILDIVTKVLAVRLLTPGQPVSIIGDTITWTLVRNSGAAFSMATGYTWVLTLIATGV
                     VIGIIWMGRRLVSPWWAIGLGMILGGAMGNLVDRFFRSPGPLQGHVVDFLSIGWWPVF
                     NVADPSVVGGAILLVALSLFGFDFDTVGRRKADDSE"
     gene            4119..5048
                     /locus_tag="VFC01_35170"
     CDS             4119..5048
                     /locus_tag="VFC01_35170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZN84897.1"
                     /translation="MTTRSMPVPEGLAGMRVDAGLARLLGLSRTAAAALAENGGVEVN
                     GAKAGKSDKLTAGAWLEVQLPEAPAPVENTPVDIEGMDILYSDADIVAVDKPPGVAAH
                     ATVGWHGPTVLGGLAAAGFRISTSGIHERQGIVHRLDVGTSGVMVVALSERAYTILKR
                     AFKQRTVEKRYHALVQGHPDPSSGTIDAPIGRHRGHDWKFAVTEHGRHSVTHYDTLEA
                     HQAASLLDIELETGRTHQIRVHFAALHHPCAGDLTYGADPVLAKKLGLERQWLHARSL
                     AFAHPADGRRVEITSPYPDDLQHALDVLRRHEL"
     gene            5045..5947
                     /gene="rarD"
                     /locus_tag="VFC01_35175"
     CDS             5045..5947
                     /gene="rarD"
                     /locus_tag="VFC01_35175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HZN84898.1"
                     /translation="MSDTRRSGLLFGVGAYASWGLFPAFFPLLKPASAFEILAHRIVW
                     CFALMVVVVAAVRRLGDLRRMSRRTWLLLAAASALISVNWVIYIYAVNNGHVVDAALG
                     YFINPLVTVALGLLIFRERLNRAQFVALAIAVIAVVILTVEVGAPPIIGIGLALSFGL
                     YGAVKKVVPVDPRVSVGVEAAIATPLALAFIAVLHGTGHGTFTDHGLGHIVLMILSGV
                     LTALPLLFFAAAAQRLPMITMGLLFYLTPAMQLTWGVVVGHEPMPPARWLGFALIWLA
                     LAVFSADALWRARVDRRALESSYP"
     gene            5976..6353
                     /locus_tag="VFC01_35180"
     CDS             5976..6353
                     /locus_tag="VFC01_35180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZN84899.1"
                     /translation="MRYCLLMHYPEGSEVGLTEEDMAPAMAAFQQYADDLSAAGVLID
                     TEVLESVVATTTVTAHTGTPEIQDGPFADTKEKLGGIFVIEVDNLDEALKWAQRNPAN
                     AWGWIEIRPVARTYAADRGWHNP"
     gene            6401..7558
                     /locus_tag="VFC01_35185"
     CDS             6401..7558
                     /locus_tag="VFC01_35185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HZN84900.1"
                     /translation="MLAVLAAPTRDIAAAEDALADALERALARWPDDGIPTNPEGWVL
                     TVARNRLRDVWKSSGYRLTESLNESNYAATSLDDEMPAIPDRRLELMLVCVHPAIAPN
                     IRTPLMLQSVLGVEAAAIATAFAVEPATMAQRLVRAKKRIRDAGIPFVRPERDDLAER
                     LPAVLEAVYGAYAIDWQLTPQGAPIESLSAEALHLALVLAELLPEEPEVLGLAALVCL
                     SEARRPARRTADGAFVPLDEQDARLWDQALIASGEALLRRAHDYRRPGRFQYEAAIQS
                     AHCSRAVQSTVDLAVLRKLHRALLRVAPSLGAAVAAAAVDGEIDGPDAGLRALDGIDD
                     PAVERFQPAWTTRAHLLAEAGRAAEAADAYRRAIELTTDPGVAEYLFQRLR"
     gene            7589..8530
                     /locus_tag="VFC01_35190"
     CDS             7589..8530
                     /locus_tag="VFC01_35190"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN84901.1"
                     /translation="MGSDELHFSNGRGRGRPVGADSAETRARILRAARDVINERDYEA
                     ANFQAIASRAGLSRPTMHYYFHTREEVFDCLVAEAYSIVADCIAQAKREDTLMNQLST
                     FVTAAQRLAFADRSMLKFTVTARLESHRCPSLRENPGPVVSAVQEFYRSVVDDAITRG
                     EIPADTDAAAVVSMLLAMFLGMGFYAGFIVDRNEMAVIAKQLHHLMTHGLLDDQRNGR
                     PSAVAPPRSDRAMVFPQVNRWQLPLQFWTKTLPFRGKHPIPRGLTGNTVLHMVGIWFA
                     VLVAAFVAAGVLTQLGWELRPMPTVKRVLRRGRSRVD"
     gene            complement(8536..8862)
                     /locus_tag="VFC01_35195"
     CDS             complement(8536..8862)
                     /locus_tag="VFC01_35195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN84902.1"
                     /translation="MTSPKIGSPVRGSRSGRPIMAALDMLGRRGALRVLWELRGDPLT
                     FRALQDACETNPGSLNTRLRELRDLGIVDHNAGGYRLTDQGRALMNALEPLQDWAHQW
                     ATAKVP"
     gene            8901..9461
                     /locus_tag="VFC01_35200"
     CDS             8901..9461
                     /locus_tag="VFC01_35200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZN84903.1"
                     /translation="MASYKFHSARIAPASGPLERDMQEAIDAIMRGAPPLVLFTTMAR
                     DRRLFFKFFNSGLLDRGHLTIRQREIVIDRVTASCGAEYEWGVHVSVYAAKAGLTEAQ
                     IASLVGGGPDDDCWDDEDRVLIRLCDGLQRSCAIDDDVWTQLTAHHSDEAILELLMLA
                     GTYRTVSYLVNGLRLPLEPGARRFPA"
     gene            9534..>12119
                     /gene="dnaE"
                     /locus_tag="VFC01_35205"
     CDS             9534..>12119
                     /gene="dnaE"
                     /locus_tag="VFC01_35205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZN84904.1"
                     /translation="MNSSFVHLHNHTEYSMLDGAAKVKPMLAEAQRLQMPAIGMTDHG
                     NMFGASEFYNAATEVGIKPIIGIEAYIAPASRFETKRILWGDPSQKADDVSGSGSYTH
                     MTMVAENAAGLRNLFRLSSLASFEGQLGKWSRMDAELIAEHAAGIIATTGCPSGEVQT
                     RLRLGHDREALESAAKWREIFGADNYFLELMDHGLDIEQRVRDGLLEIGRKLGIPPLA
                     TNDCHYVTRDAAHNHEALLCVQTGKTLSDPNRFKFDGDGYYLKSAAEMRQIWDGDIPG
                     ACDSTLLIAERVQSYADVWEQRDRMPIFPVPEGHDQASWLRHEVDAGLARRFPSGVPQ
                     EYVDRAAYEIDVICAKGYPSYFLIVADLVSYARSVDIRVGPGRGSAAGSLVAYALRIT
                     DIDPIEHGLLFERFLNPERASMPDIDIDFDDRRRGEMVRYAADKWGSDRVAQVITFGT
                     IKTKAALKDSARIHYGQPGFAIADRITKALPPPIMAKDIPLSGITDPNHERFKEAAEV
                     RGLIDTDPDVRTIYETARGLEGLIRNAGVHACAVIMSSEPLTEAIPLWKRPQDGAIIT
                     GWDYPSCEAIGLLKMDFLGLRNLTIIGDALENIKANRGIDLDLESVPLDDPATYQLLG
                     RGDTLGVFQLDGGPMRDLLRRMQPTGFEDVVAVIALYRPGPMGMNAHNDYADRKNGKQ
                     AIKPIHPELEEPLRDILAETYGLIVYQEQIMRIAQKVASYSLARADILRKAMGKKKRE
                     VLEKEFEGFSDGMKSNGFSAAAIKALWDTVLPFADYAFNKSHAAGYGLVSYWTAYLKA
                     NYPAEYMAGLLTSVGDDKDKAAVYLADCRKLGITVLPPDVNESGLNFASVGEDIRYGL
                     GAVRNV"
BASE COUNT         2008 a         4075 c         4026 g         2010 t
ORIGIN      
        1 ggagtccgag cggaccagga cgcaggacag gacctcgtcc gtggaggcgt tgatctcctg
       61 gtgcgggaca tagggcggca cgtagatgaa gtcgcccggc ccggcttcgg cggtgaattc
      121 gaggcgatcg ccccaccgca ttcgggctcg gcccgcaacg acatagatca cgctctcgag
      181 gtgcccgtgg tggtgcggtc cggtcttggc gtcgggatgg atgataaccg taccggccca
      241 tagcttctcg gcgccggcgc gcgctgcgtt gatggcggcg gcgcgattca tcccaggcgt
      301 ctgtggagta ttcggatcca attcgtcggc gtgcacgacc cgcacgccat ggaggcggta
      361 gtcggtgtgc tcatcggcca tcacaaacct ccgctcgggg atatacacct ccagaggtcg
      421 tcaacaaatg gtgacgccct acagacgagt gtcccgccgg tagagaacgg tggcgcacac
      481 cttcgcgaaa tcggccagca cgtcgcggac gaaccgggtg cgccgatagc cgatcgcgtc
      541 gagcagggga ccgtggtcga tcgcgacgac gaccgtcgcg ccgatcgcgg tcgtccacca
      601 gaacgcgaag gccagcagcc gcgccagggc gccggaccga gcgaacccga tacgcaatcg
      661 gtcctgttgg aaccgaacgt gcgggtgttc gtcggcgacg atccgccctg ccaccgcacg
      721 caccacagga tcgggcccgt cggtggccag gccgccgtag taccagagcg ccaccacctc
      781 ggcgaccgtc agcaccatca actcggtgcg cagtcccatc agccgacgca atcgcacgaa
      841 gaccgcatcc gaccagtgcc tgcgcatctg ttcgccgccg agataagcca gcaggcgcag
      901 cagcagcgcc gcatgctgtt gttcttcgcc gatgaaaagc cgtgcagcat caaggtattc
      961 ggcatctccg gcctttgcgg ccttgcgcaa tagctgggcg ccttcgccgc tctcgccgag
     1021 ctggaatcgg cggatcgacg cgatcaatgg ctggcgcacc ggttcgggga tttcgcagac
     1081 cccgtcgaag ccgatggccg cgtcggtgct ggcgtgcacg gcgtgattct gttcgaaatg
     1141 ctcgacccac aggccgaacg cacctacctc acggacctgt tgcgtcatga ccgccaggct
     1201 agcgacctag catcggcggt tgtggaggct cgctgggtgc tgcacttcga catggatgcg
     1261 ttcttcgcgt ccgtcgagca gctgacccgg cccaccctgc ggggccgacc cgtgctcgtc
     1321 ggcgggctgg gcggccgcgg cgtcgtggcc ggggccagct acgaggcccg agtgtacggc
     1381 gcccggtcgg cgatgccgat gcatcaggcc cgccgcttgg ttggcgctcc ggccgtcgtg
     1441 ctgcccccgc gcggcgtcgt ctacggcgtg gccagccgcc gcgtgttcga gaccgtccgg
     1501 acgttggtgc ctgttctgga acagctgtcg ttcgatgagg cgttcggcga gcctgccgaa
     1561 ctcgccggcg cggacgccga cgccgtcgac gcgttctgcc aacagctcaa agccaggatc
     1621 cgcgaggaga ccggcctggt cgcctccgtc ggcgccggat ccggcaaaca gatcgccaag
     1681 atcgcctccg acctggccaa acccgacggc atccgggtgg tgcgtcgcga cgaggaaagc
     1741 gttctgctgc atggccttcc ggtgcgccgg ctgtggggca tcggaccggt ggccgaggag
     1801 aaactgcatc ggctcggtat cgaaaccgtc ggagcgctcg cggcgttgac cgacgccgag
     1861 gtcgccaaca tcctcggggc gaccgtcggg cccgcgctgc accggcttgc ccagggcatc
     1921 gacgaccggc ctgtcgccga gaacgcccca gccaaacaga tcagcgccga gtccaccttc
     1981 cccgaagacc tcaccaccct cgatcaactc cgcgaagccg tggggcccat cggcgagcac
     2041 gcccacaccc gcctcgagaa ggacggccgc ggcgcccgca ccgtcaccgt caagctcaag
     2101 aagtccgaca tgagcatcct gacccggtcc gccacgctgc cgtacgcgac gaccgatgtt
     2161 tcgacattga tcgccatggc tcggcgactg ctgctcgacc ccatcgagat cggcccgatt
     2221 cgccttgtcg gcgttggttt ttcgggctta tccgacgtcc ggcaggagtc gctgtttccc
     2281 gatctcgagc tggtggccga cgacgtgccg aacaccagcc tgccgccgac cgtcaccgat
     2341 ctcgccgcga tgccgcccgc ctggcgcgtc ggcgacgacg ttgtgcacac cgaattcggc
     2401 cacggctgga tccagggcgc gggccacggg gtgatgacgg tacggttcga gacccgcagc
     2461 agcgggccag ggccggtgca cacctttccc gacgacaccc ctggcctcga gcgcgccaac
     2521 cccgtcgaca gcctggactg ggcggacttc ctcagtgagt tggccgcccg gtcagcccca
     2581 gcgggcgacg acgtcctcga caggtgagcc cgccgcgagg gcggccatca ccaacacccg
     2641 ggcctgcgcc gcaggcaacc gcggcaccac caccgcgccc gcgtccacca actccctgcc
     2701 cggcccgtag ccggcactga ccctgccgcc gggtacccgg gtcgacaccg cgactgccac
     2761 gccgtcgcgg cagtgccgcc gcaccccgtc gatcacggcc gccccggcgt tacccgagcc
     2821 gaccgcctcc agcacgatgc cgcgcgcgcc cgccgccaca cacgcggcca tcgcgaccgc
     2881 gtcagcgccc ggatacaccg cgatggtgtc cacccgcggc gcgtcagcgg ccgacagctg
     2941 gccaagaaac ggccgctcct tcgatcgctg cgcggtgaag cacccgtcgc cgaccctgcc
     3001 gagcacctcg cccgtgaagc cgcgcaggtc gcccgtcgcg gctttctgca agcccagcgg
     3061 ctgccacacc acgccggcca gggtcaccag cacaccgagg ccgcgggcgt ccgcactgcc
     3121 cgccagcgtc agcgcgtcgc gcatattccc gggcccgtcg gcatcgggtg catccgcact
     3181 gtgcagcgcg ccggtgatca ccacgggcag cgcgccgtcg taggtcagct cgagccagag
     3241 ggccgtctcc tccatggtgt cggtgccgtg ggtgatcacc acgccgtcgg cgtccgacgg
     3301 gatcgccgat cggatttgat cccagtgcgc gggcgtcaac tgcgagctgt ccaccgccat
     3361 caggtcgatg acctccacgt cgagcccgtc ggtcagctcg gcaccgctgc gcgtgggtcg
     3421 cttgaccccg tcgtggtcgc tgctggtggc gatggtgccg ccggtggtga cgacgacgag
     3481 gcgacccatg ggggaatcat cccaaaacca ctctcctgcg atgatggggg agtgactgat
     3541 gaacccaccg ggtcggccga gcccgtgatc gacggagacg ggatctccgg ctcggccgga
     3601 ggtgaggccc cgcagcctgc gccgaaccgg cggctgcgcc tgctgctcac cgttgccgcg
     3661 gtggtcctga ttctcgacat cgtcaccaag gtgctcgcgg ttcggctact gacgccgggc
     3721 cagccggtgt cgatcatcgg tgacaccatc acctggacgc tggtgcgcaa ttccggcgcc
     3781 gcgttctcga tggccaccgg ctacacatgg gtgctcacat tgatcgctac cggtgtggtg
     3841 atcggcatca tctggatggg ccgacggctg gtgtcgccgt ggtgggccat cggcctcggc
     3901 atgatcctcg gcggcgccat gggcaacctg gtcgaccggt tcttccgctc accaggaccg
     3961 ctgcagggcc atgtcgtcga cttcctgtcc atcggctggt ggccggtgtt caacgtcgcc
     4021 gatccgtcgg tggtgggtgg cgcgattctg ctggttgctt tgtcgctgtt cggttttgac
     4081 ttcgatacgg tcggccgccg caaggccgac gactcagagt gacgacccgc tcgatgccgg
     4141 ttcccgaggg gctggcgggg atgcgcgtcg acgccgggct ggcgcggctg ctgggcctgt
     4201 cgcgcacggc cgctgcggcg ctcgccgaga acggcggcgt cgaggtcaac ggcgccaagg
     4261 cgggcaagtc cgacaagctg acggcgggcg cgtggctgga ggtgcagctg ccggaagcgc
     4321 ctgcgcccgt ggagaatacg cccgtcgaca tcgagggcat ggatatcctg tattccgacg
     4381 ccgacatcgt cgcggtcgac aagccgccgg gggtggccgc ccacgcgacc gtcggatggc
     4441 acgggccgac tgtgctcggc ggccttgccg ccgcggggtt ccggatcagc acgtcgggta
     4501 tccacgagcg gcaaggcatc gtgcatcgcc ttgacgtcgg tacctccggc gtcatggtgg
     4561 tggcactctc cgaacgcgcc tacaccatac tgaagcgggc gttcaagcag cgcaccgtcg
     4621 aaaagcgtta ccacgcactg gtacagggcc atcccgatcc gtcgagcggg accatcgacg
     4681 cgccgattgg caggcaccgc ggccacgact ggaagttcgc ggtgaccgag cacggccgtc
     4741 acagcgtcac gcactacgac acgctggagg cacaccaggc ggccagcctg ctcgacatcg
     4801 aactggaaac cggtcgcacc catcagatcc gggtccactt cgccgcactg catcacccgt
     4861 gcgccggtga cctgacctac ggcgcggatc cggtactcgc gaaaaagctt gggctggaac
     4921 gccaatggct gcacgccagg tcgctggcat tcgcccatcc cgccgacggc aggcgggtcg
     4981 agatcaccag cccctatccg gacgacctgc agcacgcgct ggacgtgctg cgccgccacg
     5041 aactgtgagt gacacccggc gctcggggtt gttatttggc gttggcgcct acgcgtcgtg
     5101 gggcctgttc cccgcattct tcccgctgct gaaacccgca agcgcgttcg agatcctcgc
     5161 gcaccggatc gtgtggtgtt tcgcgctgat ggtggtggtt gtagcggcgg tgcgccgact
     5221 cggtgacctg cgccggatga gccggcgcac ctggctgttg ctcgccgccg cgtcggcgct
     5281 gatctcggtc aactgggtca tttacatcta tgcggtcaac aacggtcacg tggtggacgc
     5341 ggcgctcggc tacttcatca acccgctggt gacggtggcc ctggggctgt tgattttccg
     5401 cgaacggctc aaccgcgcgc agttcgtcgc cttggcgatc gcggtgatag cggtagtgat
     5461 cctgacggtg gaggtcggcg cgccgccgat catcggaatc ggcttggcgc tgtcgttcgg
     5521 cctctacggc gccgtcaaga aggtggtgcc tgtcgatccg cgcgtcagtg tcggagtgga
     5581 agccgcgatc gcgacgcctt tggcgctggc gttcatcgcg gtgttgcacg gcaccggcca
     5641 cggcacgttc accgaccatg gactcggcca cattgtgctg atgattctgt ccggcgtgct
     5701 gacggcactg ccattgctgt tcttcgccgc cgccgcacaa cggctgccga tgatcacgat
     5761 gggtctgctg ttctacctga caccggcgat gcagctgacg tggggcgtgg tcgtcgggca
     5821 cgaaccgatg ccgcccgccc gctggctggg cttcgcgctg atctggctgg cgctggcggt
     5881 gttcagcgcc gacgcgctgt ggcgggcccg tgtcgatcgg cgcgccctcg aatcgtcata
     5941 cccgtgacat cgtttagtca gaaaggactc acgtcatgcg gtactgcctg ttgatgcact
     6001 acccggaggg ctccgaggtc ggattgactg aggaagacat ggcgcctgcg atggcggcct
     6061 tccagcaata cgccgacgac ctgtcggctg ccggtgtgct gatcgacacc gaggtgctcg
     6121 aatccgtcgt cgccaccacc accgtcaccg cccacaccgg cactcccgag atccaggacg
     6181 ggccgttcgc cgacacgaag gagaagctgg gcggcatctt cgtcatcgag gtggacaacc
     6241 tcgacgaggc gctgaagtgg gcgcaacgca acccagccaa cgcgtggggc tggatcgaaa
     6301 tccgcccggt ggcgcggaca tacgccgccg accgcgggtg gcacaacccg tagacgcacg
     6361 ggcccgggtg gcccaggtcg cccgggattc ctacggtcgg ctgctcgccg tcctcgcggc
     6421 accgacgcgc gatatcgctg ccgccgagga cgcgctggcc gacgctctcg aacgcgcgtt
     6481 agcccgctgg cccgacgacg gtatccccac caatcccgaa ggctgggtac ttaccgtggc
     6541 acgaaaccga ttacgtgatg tgtggaagtc atcgggctat cggctgaccg aatcgctcaa
     6601 cgaaagcaac tacgctgcaa cgtctttgga tgacgaaatg cctgccattc ccgaccggcg
     6661 gcttgagctg atgctggtct gcgtacaccc tgccatcgcg cctaacattc gcacgccgtt
     6721 gatgctgcag tcggtgcttg gcgtcgaggc cgccgcgatc gccaccgcct tcgcggtgga
     6781 acccgcgacc atggcgcagc ggctggtgcg ggcgaagaaa cgcatccgcg acgcgggcat
     6841 cccgttcgtg cgtccggaac gcgacgacct cgccgagcgc ctgcccgcgg tgctggaggc
     6901 tgtgtacggc gcgtatgcga tcgattggca gctcacaccg cagggtgcac cgatcgaatc
     6961 gctgtccgcc gaggcgctgc acctggcgct cgtgcttgcc gagctgctgc ccgaggagcc
     7021 ggaggtgctc ggcctggctg cgcttgtgtg cttgtcggag gcgcgcagac ccgccaggcg
     7081 cacggccgac ggcgcgttcg tcccgctcga cgagcaggac gccagacttt gggaccaggc
     7141 gctgatcgcg agcggtgagg cgctgcttcg ccgcgcccat gactatcgca ggccgggccg
     7201 cttccagtac gaggcggcga tccagtcggc ccactgcagc agggctgtgc aaagcacagt
     7261 ggatctagcg gtgctgcgaa agctgcatcg cgcgctgctg cgggtagcgc cgtcgctcgg
     7321 cgcggccgtg gccgcggcgg cggtggacgg tgagatcgat ggtcccgacg cgggcctgcg
     7381 cgcgctggac ggaatagacg acccggctgt cgaacgattt cagcctgcgt ggacgacgcg
     7441 ggcacacctg ctggcggagg caggtcgggc ggcggaggcc gccgatgcct accgccgggc
     7501 gatcgaactg accactgatc cgggggtagc cgagtacctt ttccagcgtt tgcgttgaaa
     7561 gcgactagca atgccatagc gtttgctcgt ggggagcgac gaactgcatt tttctaacgg
     7621 tcgggggcgg ggccgtcccg tgggtgccga ttcggctgag acacgggcgc gcattcttcg
     7681 cgcggcgcgc gacgtgatca acgagcgcga ctatgaggcg gccaatttcc aggcgatcgc
     7741 gtcacgtgcc ggcctgagcc ggccgacgat gcactactac tttcatactc gcgaggaggt
     7801 cttcgactgt ctcgtggctg aggcctattc aattgtcgcg gactgcattg cgcaggcaaa
     7861 acgcgaggat accttgatga atcaactgtc gaccttcgtg acggccgcac aacggttggc
     7921 ctttgcggac cggtcgatgc tgaaattcac tgtgacagcc cggctggaat cgcaccgctg
     7981 tccgagcctt cgagagaacc ccggtccggt ggtctccgcg gtgcaggagt tctaccgatc
     8041 ggtcgtcgac gatgccatta cgcgcggtga gattccggcc gacacagacg cggcagcagt
     8101 ggtaagcatg ttgctggcga tgtttctggg gatgggtttc tacgccggat ttattgtcga
     8161 tcgaaacgag atggcagtga ttgccaagca actccatcac ctgatgactc acggcttgct
     8221 ggacgaccag cgcaacggtc gcccttcggc cgtcgctccg cccaggtctg accgggcgat
     8281 ggtgttcccg caggtcaatc gatggcagct gcccttacag ttctggacga aaactcttcc
     8341 ttttagaggg aaacacccga ttcctcgcgg cctgaccggt aataccgtgc tccacatggt
     8401 tggcatctgg ttcgcagtcc tcgtcgccgc atttgtcgcg gccggggtcc tcacccaact
     8461 cggctgggag ttgcgcccga tgcccacggt caaacgcgtg ctgcgacgcg gacggtcgcg
     8521 ggtcgactga cgccgtcagg ggaccttggc cgtcgcccat tggtgggccc agtcctgtag
     8581 cggctcaagg gcattcatca gtgcacggcc ctgatcggtg aggcggtatc cacccgcgtt
     8641 gtggtcgacg atgccgaggt ctcgaagttc gcgaagtcgg gtgttgagcg agccggggtt
     8701 ggtctcacat gcgtcctgca gggcgcggaa tgtcagcggg tcaccgcgga gttcccacag
     8761 cacgcgcagg gccccgcgcc ggccaagcat gtcgagagcc gccatgatcg gcctgcccga
     8821 cctcgaaccg cgtaccggcg atccgatctt cggcgaagtc atgcttcaca ttttgtagca
     8881 cggatgcgta gagtgccagc atggcaagct ataaattcca tagcgcgcgg atcgctccgg
     8941 cctccggtcc gctggaacgc gacatgcaag aggcgatcga cgcgatcatg cggggggcac
     9001 cgccgctggt gctcttcacg acgatggccc gtgatcggcg gctgttcttc aagttcttca
     9061 attcaggctt gctggaccgc ggccatctga cgatccggca gcgcgagatc gtcatcgacc
     9121 gcgtgacggc ctcgtgcggc gccgaatacg agtggggagt acacgtctcc gtctacgcgg
     9181 ccaaagccgg gctgaccgaa gctcagatcg cgtcgcttgt gggcggcggg cccgacgacg
     9241 actgctggga cgacgaagat cgggtgctca tccggctgtg cgacggtttg caacggagtt
     9301 gcgcgatcga cgacgacgtc tggacccaac tgaccgcgca ccacagcgac gaggccattc
     9361 tggagttgct catgctggca ggcacctacc ggaccgtgag ctatctcgtg aacgggttgc
     9421 ggctgccgct ggagccgggc gctcgccgat tcccggcgta gggcaccccc ggcaaagaca
     9481 cgcagagcga cacgccgaga tctgtcgggg cacggtcata gactggcgtc cctatgaact
     9541 catccttcgt gcacctgcat aaccacaccg aatactcgat gttggacggt gccgcgaagg
     9601 tcaagccgat gctcgccgag gcgcagcggc tgcagatgcc agcgatcggg atgaccgacc
     9661 atggaaacat gttcggcgcc agcgagttct acaacgccgc caccgaagtc ggcatcaagc
     9721 cgatcatcgg gatcgaggcc tacatcgcgc cggcctcgcg cttcgagacc aagcgcatcc
     9781 tgtggggtga tccgagccag aaggccgacg acgtctcggg cagcggttcc tacacgcaca
     9841 tgaccatggt cgccgagaac gccgcgggtc tgcgcaacct tttcaggctg tcgtcgctgg
     9901 cgtcattcga gggccagctc ggcaagtggt cgcggatgga cgccgaactc atcgccgaac
     9961 atgcggccgg catcatcgcc accaccggct gcccgtccgg tgaggtgcag acccggctgc
    10021 ggctcggcca tgaccgagag gcgctggagt cggcggccaa gtggcgcgag atcttcggcg
    10081 ccgacaacta cttcctcgaa ctgatggacc acggcctcga tatcgagcag cgggtgcgcg
    10141 acggcttgct ggagatcggc cgcaagctcg gcatcccgcc attggccacc aacgactgcc
    10201 actacgtcac ccgcgatgcc gcgcacaacc acgaggcgct gctgtgcgtg cagaccggca
    10261 agacgttgtc cgatccgaac cggttcaagt tcgacggcga tggttactac ctcaagtcgg
    10321 ccgcggagat gcgccagatc tgggatggcg acatccccgg tgcgtgcgat tcgaccctgc
    10381 tgatcgccga gcgagtgcag tcctacgccg atgtatggga acagcgcgac cggatgccga
    10441 tcttcccggt tcccgaagga cacgaccagg catcgtggct gcgacacgag gtggacgccg
    10501 ggctcgcgcg tcgctttcca tccggcgtgc cgcaggagta cgtcgaccgc gcggcctatg
    10561 agatcgacgt catctgcgcc aaggggtacc cgtcgtactt cctgatagtg gcggacctgg
    10621 tgagctacgc gcgctcggtc gacatccggg tcgggccggg ccgcggctcg gcggccggct
    10681 cgctggtcgc ctatgcgctg cgcatcaccg acatcgaccc gatcgaacac gggctgctgt
    10741 tcgagcggtt cctgaacccc gagcgcgcgt cgatgcccga tatcgacatc gacttcgacg
    10801 accgcaggcg cggtgagatg gtccgctacg ccgccgacaa gtgggggagc gaccgggtcg
    10861 cccaggtcat cacgttcggc accatcaaaa ccaaagcggc gctgaaggat tcggcccgca
    10921 tccactacgg ccagccgggt ttcgcgatcg ccgatcggat caccaaggcg ctgccgccgc
    10981 cgatcatggc caaggacatt ccgctgtccg ggatcaccga cccgaaccac gagaggttca
    11041 aggaggccgc cgaagttcgc gggctcatcg acaccgaccc cgacgtgcgc accatctacg
    11101 agacggcgcg cggcctggag ggtctgatcc gcaacgccgg cgtgcacgcc tgcgcggtga
    11161 tcatgagcag cgagccgctg accgaggcca tcccgctgtg gaagcggccg caggacggcg
    11221 cgatcatcac cggctgggac tacccgtcgt gcgaggccat cggcctgttg aagatggact
    11281 tcctgggcct gcgcaacctg acgatcatcg gcgacgcgct ggagaacatc aaggccaaca
    11341 ggggaattga cctcgacctc gaatcggttc cgctcgacga tcccgccacc taccaactgc
    11401 tcggccgcgg cgacacgctg ggcgtgttcc agcttgacgg cgggccgatg cgcgacctgc
    11461 tgcgccgcat gcagccaacc ggattcgaag acgtcgtcgc ggtcatcgcg ctgtaccggc
    11521 cgggaccgat gggcatgaac gcccataacg actacgccga ccgcaagaac ggcaagcagg
    11581 ccatcaagcc catccatcca gagctcgaag agccgctgcg cgacatcctc gctgagacct
    11641 acggcctgat cgtctaccaa gagcagatca tgcgcatcgc gcagaaggtg gccagctact
    11701 cgctggctcg agcagacatt ctgcgaaaag ccatgggcaa gaagaagcgt gaggttctgg
    11761 agaaggaatt cgagggcttc tccgacggca tgaagtccaa cgggttctcg gccgcggcca
    11821 tcaaggcgct ctgggacacc gtgctgccgt tcgcggacta cgcgttcaac aagtcacacg
    11881 ccgcgggcta cggcctcgtg tcgtattgga cggcgtatct gaaggccaac tacccggcgg
    11941 agtacatggc tgggttgctc acctcggtgg gcgacgacaa ggacaaggcc gcggtgtacc
    12001 tcgcggattg ccgcaagctg ggcataaccg tgctgccgcc ggacgtcaac gagtcaggcc
    12061 tgaacttcgc ctcggtcggc gaggacatcc gatacgggct cggcgcggtg cgcaacgtc
//
LOCUS       DASRJA010000779         9553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_407740,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000779 DASRJA010000000
VERSION     DASRJA010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9553
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407740"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /gene="lysX"
                     /locus_tag="VFC01_35210"
     CDS             complement(<1..1706)
                     /gene="lysX"
                     /locus_tag="VFC01_35210"
                     /EC_number="2.3.2.3"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     synthetase/lysine--tRNA ligase LysX"
                     /protein_id="HZN84905.1"
                     /translation="MTATLTRPTSRYSWVPAAAGWTVGIIATLSLVASVSPGIRWIIK
                     EPREFVNDYIFNFPDTSFAWAFVLALLAAALAARKRIAWWILVGYMVAAIGWNVGDLV
                     TGDESVIRDMGEVIGLGFHVAAILFLLLARKEFWAKVRRGALLKAAAALVAAMAIGTL
                     IGWALLEFSPGSLAREDRFWYALNRVSAFAGADSSNFSGHPHVFVNALLGLFGALALM
                     VAAIVLFQSQRADNALTGEDESAIRGLLELFGKNDSLGYFATRRDKAVVFAPNGRAAI
                     TYRVEVGVCLASGDPVGDPKSWPQAIEAWLGRCQTYGWAPGVMGASSTAAEAFRQAGL
                     NALQLGDEAILHPDNFRLSGPDMRAVRQAVTRARRAGASVRIRRHRDLGSEEMAQVIK
                     RADAWRDTETERGFSMALGRIGDPADGDCVLVEAIQHETVVAMLSLVPWGTNGVSLDV
                     MRRSPKSPNGTIELMVSELCMQSEDIGVTRISLNFAMFRSAFEQGAQLGAGPVARLWR
                     GLLVFFSRWWQLETLYRSNMKYQPEWVPRYACYEDARLIPRVGVASVIAEGFLVLPFS
                     RR"
     gene            complement(1751..2086)
                     /locus_tag="VFC01_35215"
     CDS             complement(1751..2086)
                     /locus_tag="VFC01_35215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HZN84906.1"
                     /translation="MTEVPDIRELAEIPAVEVITRAAVMLMSAAAEKLGLSAEDPDES
                     PQRDLDEARRLITALAGLVTASAEYLGPHAGPVRDGLKTLQLAFRESSVVPDEPGQGP
                     GEKYTGPVW"
     gene            2287..2898
                     /gene="infC"
                     /locus_tag="VFC01_35220"
     CDS             2287..2898
                     /gene="infC"
                     /locus_tag="VFC01_35220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZN84907.1"
                     /translation="MSTETRVNERIRVPEVRLIGPGGEQVGIVRIEDALRVAADADLD
                     LVEVAPNARPPVCKIMDYGKYKYETAQKARESRKNQQQTVVKEQKLRPKIDDHDYQTK
                     KGHVVRFLEAGSKVKVTIMFRGREQSRPELGYRLLQRLGADVADYGFVETSAKQDGRN
                     MTMVLAPHRGAKTRAKAAHDADAPAAQRSSAEPSETQTDTSQN"
     gene            2907..3101
                     /gene="rpmI"
                     /locus_tag="VFC01_35225"
     CDS             2907..3101
                     /gene="rpmI"
                     /locus_tag="VFC01_35225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZN84908.1"
                     /translation="MPKAKTHSGASKRFRLTGTGKVVRQKANRRHLLEHKPTKRTRRL
                     DGRTEVSANDTKRIKKLLNG"
     gene            3144..3533
                     /gene="rplT"
                     /locus_tag="VFC01_35230"
     CDS             3144..3533
                     /gene="rplT"
                     /locus_tag="VFC01_35230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZN84909.1"
                     /translation="MARVKRAVNAQKKRRTVLKASKGYRGQRSRLYRKAKEQQLHSLT
                     YAYRDRRARKGEFRKLWISRINAAARANDITYNRLIQGLKAAGVEVDRKNLAEIAVSD
                     PAAFTALVEVAKGALPEDVNAPSGEAA"
     gene            3557..4297
                     /locus_tag="VFC01_35235"
     CDS             3557..4297
                     /locus_tag="VFC01_35235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZN84910.1"
                     /translation="MAAAIKLHRHVGRRRAASFLAEGPNLVEAALRRGLVSEVFATEA
                     ALARFSSMLGDVHVDLVTERAAKALSDTVTPIGLVAVCSVPEVALADVLPARLVAVAV
                     EISEPGNAGTLIRIADAMGADAVVLAGRSVDPYNGKCLRASAGSIFSIPVVSAPDAND
                     VVAALSETGIQVFATTVDGEVSLEYADLSQPTAWLFGPEAHGLPADLAAKATHRIRIP
                     MPGNAESLNVASAAAICLYQSARAHRVS"
     gene            complement(4328..5557)
                     /locus_tag="VFC01_35240"
     CDS             complement(4328..5557)
                     /locus_tag="VFC01_35240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN84911.1"
                     /translation="MLEWSDVDLAVRDAVREFVGKEIRPHVDALESGEMEPYPIIRKL
                     FATFGIAEMARDSLNKRLARLREGGESSGRSSGGGMFGEGAGSMGFVVVSELCRVSMG
                     IVTGMGVSLGLTVPTIMGRGTLAQQERWLPDLVTYDKIGAWAITEPDSGSDAFGGMKS
                     YVVRDGDDYILNGQKTFITNGPDADVVVVYAKLDEPGVDKRDRKVLTFVLDRGMEGFV
                     QSKPFRKMGIHSSRTGELFFNNVRLDRDRLLGESEDNAAGDGRASARANFSAERIGVA
                     AMALGVIEECLRLCVDYAKNRTLWGQEIGQFQLIQLKLANMEVARMNVRNTLFRVIEA
                     SQTGKQISLPEASAIKWYCSQAATDVAMDAVQLFGGNGYMTEYRVEQLARDAKSLMIY
                     AGSNEVQITHVAKGLLS"
     gene            complement(5562..6575)
                     /locus_tag="VFC01_35245"
     CDS             complement(5562..6575)
                     /locus_tag="VFC01_35245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZN84912.1"
                     /translation="MTTANQGDPLGPDSLTWKYFGDLRTGMLGVWIGAIQNMYPELGA
                     GVEDHSILLREPLQRVARSVYPIMGVVYDGDRAAETGEQIKSYHKTIKGVDGEGRRYH
                     ALNPETFYWAHATFFMLIIKTAEYFCGGLTEADKQQLFDEHVQWYRMYGMSMRPVPNS
                     WEEFQEYWDRKCREELEINKATLDIFTIRIPKPWFVLMPTAVWDQLFKPMVGAQRWIA
                     AGLFDEAVREKAGMRWTPGDEVVLRLFGKLVELAFVVVPEEIRLHPRALAAYRRAEGR
                     APEDAPLVEAPAFMSPPPDRRDLPMHYVPPRKTLLDRAGSLVHTTFSLAGLRPARGRG
                     KAA"
     gene            6836..7654
                     /locus_tag="VFC01_35250"
     CDS             6836..7654
                     /locus_tag="VFC01_35250"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZN84913.1"
                     /translation="MRWLKDTNHSPAVVAFLRRARRALPGDPEFGDPLSVDGVGGPRV
                     AARAADRLLEREAASREVSLGALQVWQALTERVSGKPANREVTLVFTDLVGFSAWSLS
                     VGDEATLKLLRRVSQVVEPPLLEAGGHIVKRMGDGIMAVFSEPLTAVRAAIAARDAVK
                     AVDVDGYTPRMRVGIHTGRPQRIGSDWLGVDVNIAARVMERATRGELVVSAPTLERIP
                     DEDFESLGVIAKRLRRQVFTAKQDGVPADLTMYRLKTRRQLPGSEDEQAAEPGA"
     gene            7665..8456
                     /locus_tag="VFC01_35255"
     CDS             7665..8456
                     /locus_tag="VFC01_35255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid-like protein"
                     /protein_id="HZN84914.1"
                     /translation="MLRRLLARLARIRVTVAYAVIVTSVTVVLCALGPKVQDQVIRHV
                     STNLHNLSRGHVGTLLGSAFVIDAGPIYVWLPGLVCLLAVAELTYRSGLLVVAFTAGH
                     IGATLLVAAGLTAAVELGWLPLSVSRATDVGMSYGAAAVLGSLTAAIPARWRPAWIGW
                     FLAVGAAVVAVGRDFTDVGHTVALVLGMLVATRFRRPPHWTAVRFVMLGVAAAFGYLV
                     LASTDLLVATAAGLAGAVIGEALTRRRVPLVPAGNACEVPSGHRL"
     gene            8464..9507
                     /gene="pheS"
                     /locus_tag="VFC01_35260"
     CDS             8464..9507
                     /gene="pheS"
                     /locus_tag="VFC01_35260"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZN84915.1"
                     /translation="MADQPIDLSEEALTKAVSAAQHAFDLTTDLDELARAKTEHLGDR
                     SPLALARQALGSLPKADRADAGKRVNVARTEAQRAYDERLAALRAERDAAVLVAERID
                     VTLPSTRQPIGARHPITILAEHIADTFVAMGWELAEGPEVETEQFNFDALNFPPDHPA
                     RSEQDTFYVAPDGSRQVLRTHTSPVQIRALLERELPVYIISIGRTFRTDELDATHTPV
                     FHQVEGLAVDKGLTMAHLRGTLDAFARSEFGPQGRTRFRPHFFPFTEPSAEVDIWFPN
                     KKGGAGWVEWGGCGMVNPNVLRACGIDPAVYSGFAFGMGLERTLQFRNGIPDMRDMVE
                     GDVRFSLPFGVGA"
BASE COUNT         1642 a         3185 c         3143 g         1583 t
ORIGIN      
        1 ttgcgccgcg aaaacggcag cacgagaaag ccttcggcga tcacagacgc cacaccgacg
       61 cgggggatca gccgggcgtc ctcgtagcac gcgtagcgcg gcacccactc gggttggtac
      121 ttcatgttcg accggtacag cgtctcgagt tgccaccagc gggagaagaa cacgagcagg
      181 ccgcgccaga gtcgcgccac gggccccgcg ccgagctggg cgccctgttc gaacgccgat
      241 cggaacatcg cgaagttcag cgagattcgg gtcaccccaa tgtcttcgga ctgcatgcac
      301 agctcgctga ccatcaattc gatggtgccg ttgggtgact tgggggaccg gcgcatcacg
      361 tcgagcgaca cgccgttggt gccccacggg accagcgaca gcatcgctac gacggtctcg
      421 tgttggatgg cctcgaccag cacgcagtcg ccgtcggcgg ggtcaccgat ccggcccagt
      481 gccatcgaga agccacgttc ggtttcggtg tcccgccagg catctgcccg cttgatcacc
      541 tgggccatct cctcgctgcc gaggtcgcga tgtcgccgga ttcgaacact ggcgcctgcc
      601 cgccgcgccc gggtcacggc ctggcgcacg gcacgcatgt ccggacccga aagtcggaag
      661 ttgtccgggt gcaggatcgc ctcgtcgccc agttgaagtg cgttgaggcc ggcctgccgg
      721 aaggcttctg ccgcggttga actggcgccc atcacgccgg gtgcccagcc gtaggtctgg
      781 caccgcccca gccacgcctc gattgcctgc ggccaggact tcggatcgcc gaccgggtcg
      841 ccgctggcca ggcagacgcc gacctccacg cggtaggtga tggccgcacg gccattcggt
      901 gcgaacacca ccgccttgtc ccggcgggtg gcgaagtagc ccagtgagtc gttcttgccg
      961 aacagttcga gcagaccccg gatcgcggac tcgtcctcac ccgtaagggc gttgtcggcg
     1021 cgctgcgact ggaacagcac gatcgccgcc accatgagtg cgagcgcgcc gaacaggccg
     1081 aggagcgcgt tgacgaagac gtgcgggtgt ccggagaagt tcgaagaatc ggcgccggcg
     1141 aacgcgctga ctcggttcag cgcgtaccag aaccggtcct cacgggccag cgagccggga
     1201 gagaactcga gcagggccca cccgatgagc gtgccaatcg ccatggcggc gacaagggcg
     1261 gcggccgcct tcaacagtgc accgcgacgg accttcgccc agaactcctt gcgggccagc
     1321 agcaggaaca agatcgccgc gacatggaaa ccgagaccga tcacctcgcc catgtctcgg
     1381 atgaccgatt cgtcgccggt cacgagatcg ccgacgttcc agccgatcgc cgcgaccatg
     1441 tagcccacca gaatccacca cgcgatgcgc ttgcgggccg cgagcgccgc ggccaacagc
     1501 gccaacacaa aggcccacgc gaagctggta tcggggaagt tgaagatgta gtcgttgacg
     1561 aactcgcgcg gctccttgat gatccagcgg atgccgggcg agacgctggc aacgagggac
     1621 agcgtggcga tgatgccgac ggtccagccg gccgccgcag gcacccatga gtaccgagaa
     1681 gtcgggcggg tcaacgtggc ggtcataggc cgcgagaata tgcgctaaac cggtgaaatg
     1741 ggtgggagtg ctaccagacg ggcccggtgt acttctcgcc cgggccctgg cccggttcgt
     1801 ccgggacgac actcgattcg cggaacgcca actgcagtgt cttcaacccg tcgcgcaccg
     1861 gcccggcgtg cggaccgaga tattcggcgg aggcggtgac caacccggcc agtgcggtga
     1921 tcagacggcg cgcctcgtcg agatcgcgtt gtgggctttc gtccggatct tccgctgaca
     1981 gcccgagctt ctccgcagcg gcgctcatca gcatcacagc cgcgcgggtg atcacctcga
     2041 ccgcgggaat ttcggccagc tcacggatgt caggaacctc ggtcatgcct gctagactgg
     2101 catgcgcgac cgtcccggcg tacgccaggg acagcaagtg gagtcccact cccaccgctg
     2161 accataccgg tgcagcggtc cggtcacagg catccgatgc ctgtcctggt cggctttggg
     2221 ccctgctttg agcagggcct tttcgctgat ggtgtggggc tcctagagga caacatagga
     2281 ggccccatca gcactgagac ccgcgtcaac gagcgcattc gagtccctga agtccgctta
     2341 atcggaccag gtggcgagca ggtaggcatt gtgcgcatcg aagacgccct ccgcgtcgcc
     2401 gcggatgccg atctcgacct tgtcgaagtt gccccgaacg ccagaccccc ggtctgcaag
     2461 atcatggact acggcaagta caagtacgag acggctcaga aggcacgcga gtctcgcaag
     2521 aatcagcagc agaccgtcgt caaggaacag aagctgcgac ccaagatcga cgatcacgac
     2581 taccagacca agaagggcca cgtggtccgc ttcctggaag cggggtcgaa ggtcaaggtg
     2641 accatcatgt tccgcggacg cgagcagtcg cggcccgagc tgggctaccg actgctgcag
     2701 cggctgggcg ccgacgtcgc cgactacggc ttcgtcgaga cgtcggccaa gcaggacggc
     2761 cgcaacatga cgatggtgct ggctccgcac cgcggcgcga agactcgcgc caaggcggcg
     2821 cacgacgccg acgcaccggc tgcgcagcgc agcagtgccg agcccagcga aacacagacc
     2881 gatacatcac agaactgagg acatctatgc ccaaggcgaa gacccacagc ggcgcttcga
     2941 agcggttccg gctgaccggg accggcaagg tcgtgcgcca gaaggccaac cgtcggcatt
     3001 tgcttgagca caagccgacc aagcgcaccc gccggctcga cggccgcacc gaggtgtcgg
     3061 ccaacgacac caagcgcatc aagaagttgc tgaacggcta gttagcccgg ccccgtacga
     3121 cctgaacgaa taggaacact ctcatggcac gcgtcaagcg cgcagtcaac gctcaaaaga
     3181 agcggcgcac agtactcaag gcatccaagg gctaccgcgg tcagcggtcg cggctttacc
     3241 gcaaagccaa agagcagcag ctgcattcgc tgacctatgc ctaccgtgac cggcgcgccc
     3301 gcaagggcga gttccgcaag ctgtggatct cccggatcaa cgccgccgcc cgcgccaacg
     3361 acatcaccta caaccggctg atccagggcc tgaaggctgc cggtgtcgag gtagaccgca
     3421 agaacctcgc cgagatcgcg gtcagcgacc ccgccgcctt caccgcgctg gttgaggtcg
     3481 ccaagggcgc gttgcccgag gacgtcaatg cgccgtcggg cgaggctgcc tgacgctcac
     3541 cgagcgctcc aaccgggtgg cggcagcgat caagctgcat cgccacgtcg gacgacgccg
     3601 cgccgcaagc tttctcgccg agggacccaa tctcgtcgag gcagcgttgc ggcgcggact
     3661 cgtttctgag gtgttcgcca ccgaagcggc gctggctcgc ttcagctcga tgctgggcga
     3721 cgtccacgtc gatctggtga ctgagcgcgc cgcaaaagcg ttgtccgaca ccgtgacccc
     3781 gatcggattg gtggcagtct gctcggtgcc cgaggtggca ttggctgacg tgctcccggc
     3841 ccggctggtg gccgttgcgg tggagatttc cgagcccggc aatgccggga cgttgattcg
     3901 catcgccgac gcgatgggcg ccgatgccgt cgtgctagca gggcgcagcg tggatcccta
     3961 caacggaaag tgcctgcggg cgtcggccgg cagcatcttc tcgattccgg tggtgtcggc
     4021 acccgacgca aacgatgtcg ttgccgcatt gagcgaaacc ggaatacagg tgttcgccac
     4081 caccgtcgac ggcgaagtca gtctcgagta tgcggacctg tcgcagccga cggcctggct
     4141 gttcgggccc gaggctcatg ggttgcctgc ggatttggcg gccaaggcga cgcatcgcat
     4201 tcgcattccg atgccgggaa atgccgaaag cctcaacgtg gcgtcggccg cggcgatctg
     4261 cctgtaccag agcgcgcggg cgcaccgggt gtcctagcgt tccccagcat cgtgggccca
     4321 gttcgcgcta actcaatagc cccttggcga cgtgggtgat ctgcacctcg ttgctgccgg
     4381 cgtagatcat caacgacttg gcgtcgcggg ccagctgctc cacccggtac tcggtcatgt
     4441 agccgttgcc gccgaacagc tgcacggcat ccatcgccac atcggtggcg gcctgcgagc
     4501 aataccactt gatcgccgac gcctccggca gcgagatctg cttacccgtt tgcgaggcct
     4561 cgatgacccg aaacagcgtg ttgcgcacgt tcattcgggc cacttccatg ttggccagct
     4621 tgagctggat cagctggaac tggccgatct cctggcccca cagcgtgcgg ttcttcgcgt
     4681 agtcgacaca tagtcgcagg cactcctcga tgacaccgag cgccatcgcg gccactccga
     4741 tgcgctcggc ggagaagttc gcgcgggcac tggcacgccc gtcacccgcg gcgttgtcct
     4801 ctgattcacc caatagccga tcccggtcca gccggacatt gttgaagaac aattccccgg
     4861 tgcgggagct gtgaatgccc atcttgcgga acggctttga ctgcacgaag ccctccatgc
     4921 cgcggtccag cacgaaggtc agcaccttgc ggtcgcgctt gtcgacaccg ggttcgtcga
     4981 gcttggcgta gaccaccacg acatcggcgt cggggccgtt ggtgatgaac gtcttctgcc
     5041 cgttgaggat gtaatcgtcg ccgtcgcgca ccacatacga cttcatcccg ccgaacgcgt
     5101 ccgacccgga gtccggctcc gtgatcgccc aagccccgat cttgtcgtag gtgaccaggt
     5161 ctggcagcca gcgctcctgc tgcgcgagcg tgccgcgacc catgatcgtc ggtaccgtca
     5221 gcccgagact gacgcccatg ccggtgacga tgcccatcga cacccggcac agctcgctga
     5281 ccacgacgaa gcccatgctc ccggcgccct cgccgaacat gccgccaccc gacgacctac
     5341 cggacgactc gccaccctcg cgcaaccggg ccaaccgctt gttcagcgaa tcacgcgcca
     5401 tctccgcgat gccgaacgtc gcgaacagct tgcgaatgat ggggtagggc tccatctcgc
     5461 cgctttccag cgcgtcgacg tgtgggcgaa tttccttgcc gacgaactcg cgcacagcat
     5521 cgcgcacggc gaggtcgaca tcagaccact cgagcatgca atcaggctgc cttaccgcgg
     5581 ccgcgtgccg gacgcagacc cgccaacgag aacgtggtgt gcaccagcga cccggcccga
     5641 tcgagcagcg tctttcgcgg cggcacatag tgcatcggta ggtcgcgcct atcaggcggc
     5701 ggcgacatga acgcgggcgc ctcgacgagc ggggcgtcct cgggcgcgcg gccctcggcg
     5761 cggcggtagg cagccagtgc tcgtgggtgc agccgaattt cctccggaac cacaacgaac
     5821 gccaactcca cgagcttgcc gaacagccgc agcaccacct cgtcgccggg tgtccaccgc
     5881 atgccggcct tttcgcgtac cgcctcgtcg aacagcccgg ccgcgatcca acgctgtgcg
     5941 ccgaccatcg gcttgaacag ctggtcccac accgccgtcg gcatcaacac aaaccacggt
     6001 ttggggatgc ggatcgtgaa gatgtccagc gtcgccttgt tgatctcgag ctcttcgcgg
     6061 cacttgcgat cccaatactc ctggaattcc tcccaggagt tgggcaccgg ccgcatgctc
     6121 atgccgtaca tccggtacca ctgcacgtgt tcgtcgaaca gctgctgctt gtcggcctcg
     6181 gtcagcccgc cgcagaagta ctcggcggtc ttgatgatca gcatgaagaa ggtcgcgtgg
     6241 gcccagtaaa aggtctccgg gttcagcgcg tgatagcggc ggccttcgcc gtcgacgccc
     6301 ttgatcgtct tgtggtagct cttgatctgc tcaccagtct ccgcagcccg atcgccgtcg
     6361 tagaccacgc ccatgatcgg gtagaccgag cgcgccaccc gctgcagggg ctcgcgcagc
     6421 aggatggagt gatcctcgac gccggcaccg agctcgggat acatgttctg gatcgcaccg
     6481 atccacacgc cgagcatgcc ggtgcgcagg tcgccgaagt acttccacgt cagcgagtcg
     6541 gggcccagag ggtcaccctg attggcggtc gtcattgagc gcacagctaa acgataatgt
     6601 tgacaacaca cgttgtctac gatttggcag ggcgtggcgg cccagtgtta tttacgctcc
     6661 atcctcgcgc cacaggcttg taaccaggcg gtttgccgct cgattgccgg tgccgaacag
     6721 cgccgcatac cctggcggcg tgaatgtcga gtcgttcgac gaacgatccg gcggtatcgg
     6781 ggaaatcgac caggaaccgc accgtccggc ccagccgcca cggtcatcgg caccgatgag
     6841 gtggctgaag gacaccaacc acagcccggc tgtcgtcgct ttcctgcgcc gtgcccggcg
     6901 cgcgctgccg ggcgaccccg aattcggcga tccgttgtcg gtggacggag tcggggggcc
     6961 gcgcgtcgcc gccagggccg ccgatcggct gctggagcgt gaggccgcct cacgcgaagt
     7021 gagcctgggc gcgctgcagg tgtggcaggc cctgaccgag cgtgtgtcgg ggaagccggc
     7081 gaatcgtgag gtgacgcttg tctttaccga tctggtgggc ttctcggcgt ggtcgttgag
     7141 cgtcggcgac gaggcgacgt tgaagttgtt gcgccgcgtt tctcaggttg tcgagccgcc
     7201 gctgctggag gcgggcggtc acatcgtcaa gcggatgggc gacggcatca tggcggtgtt
     7261 ttccgaaccc cttactgcgg tgcgcgccgc catcgctgct cgcgatgccg tgaaagccgt
     7321 tgacgtggac gggtatacgc cgcggatgcg ggtcggcatc catacgggcc gaccacagcg
     7381 gatcggatcg gactggctcg gcgtggacgt caacatcgcg gcgcgggtga tggaacgggc
     7441 caccaggggc gaactggtgg tttctgcgcc gacattggag cggattcccg acgaggactt
     7501 cgaatcgctg ggtgtgatcg ccaaacggct tcgcaggcaa gtctttacgg ccaagcaaga
     7561 cggtgtgccg gccgacctca ccatgtatcg cttgaaaact cgcaggcagt tgccaggtag
     7621 cgaggacgag caggcggccg aaccgggcgc atagtggatt ggagatgttg cgccgactgc
     7681 tcgcccgcct ggcacggatc cgcgtcaccg tggcgtacgc ggtgatcgtg acgtccgtca
     7741 ccgtcgtttt gtgcgcgctt ggtcccaagg tgcaggacca agtgatccgc catgtcagta
     7801 ccaacctgca caaccttagc cgtgggcacg tcggcacttt gctgggtagc gcgttcgtca
     7861 tcgacgcggg gccgatctac gtgtggctac ccggattggt gtgtctgctc gcggttgcgg
     7921 agctgacgta tcgcagcggc ctgttggtgg tggcgttcac ggcggggcac atcggtgcca
     7981 cgctgcttgt ggccgcaggc ctgacggcgg cggtggagct gggctggctg ccgctgtcgg
     8041 tcagtcgcgc gacggacgtc ggcatgagct atggcgccgc ggccgtgctt ggctcgctga
     8101 ccgcggcgat cccggcacgt tggcgaccgg catggattgg atggttcctg gcggtcggag
     8161 cggcggtggt ggccgtcggc cgcgatttca ccgacgtcgg acacacggtg gcgctggtgc
     8221 tgggcatgtt ggtcgcgacg cggttccgtc ggcccccgca ctggacggcg gtgcggttcg
     8281 tgatgctcgg cgtcgcagca gccttcggct acctggtgct ggccagcacc gatctgctgg
     8341 tcgcgacggc cgccggcttg gctggcgctg tgatcggcga ggcactcacc cggcgccgcg
     8401 tgccgctggt accggccggt aatgcatgcg aggtgccatc gggtcatcgc ctatgatcgc
     8461 cgggtggctg atcagcccat agacctgtca gaagaagcgc tgaccaaagc cgtcagcgcg
     8521 gcccagcatg ccttcgacct gaccaccgat ctggacgaat tggcccgcgc caagaccgag
     8581 catctcggcg accgctcgcc gctcgcgctg gctcgccagg cgctgggttc gctgcccaag
     8641 gcggaccgtg cggacgccgg caagcgggtc aacgtcgcgc gcaccgaggc gcaacgcgcc
     8701 tacgacgagc gtctcgccgc cctgcgcgcc gagcgcgacg ccgccgtgct ggtcgccgag
     8761 cgcatcgacg tcacgctccc gtcgactcgc cagccgatcg gcgcccggca tccgatcacc
     8821 atcctggccg agcacatcgc cgacacgttc gtcgcgatgg gctgggagct cgcagaggga
     8881 cccgaggtcg agaccgagca gttcaacttc gacgcgctga acttcccgcc cgaccatccg
     8941 gcgcgcagcg aacaggacac cttctacgtc gcgcccgacg ggtcgcggca ggtgctgcgc
     9001 acgcacacct cgccggtgca gatccgtgcg ctgctggagc gcgagctgcc ggtgtacatc
     9061 atctcgatcg gccgcacctt ccgcaccgac gaattggacg ccacccacac cccggtcttc
     9121 catcaggtcg agggtctggc ggtggacaag ggcctgacca tggcgcatct gcgcggcacg
     9181 ctggacgcgt tcgcccgctc ggagttcgga ccgcagggcc gcacccgatt ccgcccgcac
     9241 ttcttcccgt tcaccgagcc gtcggccgag gtcgatatct ggttccccaa caagaagggt
     9301 ggggccggct gggtcgagtg gggcggctgc ggcatggtca acccgaatgt gctgcgcgcc
     9361 tgcggtatcg accccgcggt gtactcgggg ttcgcgttcg ggatggggtt ggagcgaacc
     9421 ctgcagttcc gcaacggaat tcctgacatg cgcgacatgg tcgagggcga cgtccggttc
     9481 tcgctgccgt tcggggtcgg tgcctgatgc ggattccgtt cagctggctg cgtgaggccg
     9541 ttcgtgccgg cgc
//
LOCUS       DASRJA010000780         5802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1059221,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000780 DASRJA010000000
VERSION     DASRJA010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059221"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="VFC01_35265"
     CDS             <1..169
                     /locus_tag="VFC01_35265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007300045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZN84916.1"
                     /translation="GDLLGVAATGAYCYSMSSRYNLIGRPAVVAVRDGQARLILRRET
                     VDDLLSLEVTE"
     gene            166..1491
                     /locus_tag="VFC01_35270"
     CDS             166..1491
                     /locus_tag="VFC01_35270"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZN84917.1"
                     /translation="MNGDEKPIGVAVLGLGNVGSEVVRIIEDSANDLTARIGAPLVLR
                     GIGVRRVADNRGVPVELLTDNVEELVSRDDVDVVVELMGPVEPARKAILSALEQGKSV
                     VTANKALMAVSTGELAQAAEHAHVDLYFEAAVAGAIPVIRPLTQSLAGDTVLRVAGIV
                     NGTTNYILSEMDSTGADYATALADASALGYAEADPTADVEGYDAAAKAAILASIAFHT
                     RVHADDVYREGITKVTPADFASARSLGCTIKLLAICERLTTDEGQQRVSARVYPALVP
                     LDHPLAAVNGAFNAVVVEAEAAGRLMFYGQGAGGAPTASAVMGDVVMAARNRVQGGRG
                     PRESKYAKLPISPIGFIPTRYYVNMNVADRPGVLSAVAAEFGKREVSIAEVRQEGMVD
                     EGGQRCGARIVVVTHQATDAALSETVAALADLDVVQSVNSVLRMEGTTE"
     gene            1488..2570
                     /gene="thrC"
                     /locus_tag="VFC01_35275"
     CDS             1488..2570
                     /gene="thrC"
                     /locus_tag="VFC01_35275"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011781502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZN84918.1"
                     /translation="MTAPRTAVHQPWPGLIAAYRDRLPVQDNWTAITLLEGGTPLVPA
                     PRLSEYTGCTVHLKVEGLNPTGSFKDRGMTMAVTEAVARGQQAVLCASTGNTSASAAA
                     YAARAGITCAVLIPQGKIAMGKLAQAVMHGAKIIQIDGNFDDCLELARKLTADYPTIS
                     LVNSVNPYRIEGQKTAAFEIVDALGSAPDVHSLPVGNAGNITAYWKGYTEYHRDGVSD
                     RLPRMLGTQAAGAAPLVLGEPVSNPETIATAIRIGSPASWASAVEAQQQSNGRFLAAT
                     DEEILAAYHLVARTEGVFVEPASAASIAGLLKSIEDGWVTRGSTVVCTVTGNGLKDPD
                     TALKGMPAVTPVPVDPSAVVEKLGLV"
     gene            2575..3516
                     /gene="thrB"
                     /locus_tag="VFC01_35280"
     CDS             2575..3516
                     /gene="thrB"
                     /locus_tag="VFC01_35280"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HZN84919.1"
                     /translation="MNTLPAGLTATAVVAASSANLGPGFDSLGLALSLYDEIVVETTE
                     SGLVVEVEGEGAGQVPQDSSHLVIRAIEHGLQAVGVTVPGLNLRCRNDIPHSRGLGSS
                     AAAVVGGLAAVNGLVTQAGSTELSESELIQLASEFEGHPDNASAAVLGGAVVSWTEHE
                     GVLPRYAAAPLRVHPDIHLFPAIPEERSSTAETRVLLPDRVSHTDARFNLSRAALLVL
                     ALTERPDLLMEATEDVLHQPQRAPAMPASAEYLRILRRCGVAAVLSGAGPAVIALSSQ
                     AELPAEALEYGTANGFTVSKMAVGEGVRWTSGVAVRS"
     gene            4003..5676
                     /gene="rho"
                     /locus_tag="VFC01_35285"
     CDS             4003..5676
                     /gene="rho"
                     /locus_tag="VFC01_35285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZN84920.1"
                     /translation="MVLPELRALAKQIGVEGASGMRKSELVAAIRERRGESNGRPKEA
                     ASSDATDSAAPAADTTEQPAEEQAPPRRERRGASRGAGAPGGPDEKKGETKQESAQQN
                     KADTEAAQAPKQDRKSDGGDQQGDQGGQNRGGNAGDDDGEGRGGRRGRRFRDRRRRER
                     GDGGGGDRDTELREDDVVQPVAGILDVLDNYAFVRTSGYLAGPNDVYVSMNMVRKNGL
                     RRGDAVTGAVRVPKDGEQPNQRQKFNPLVRLDTVNGKPVEEAKKRPDFTKLTPLYPNE
                     RLRLETTSDKLTTRVIDLIMPVGKGQRALIVSPPKAGKTTILQDIANAITKNNPECHL
                     MVVLVDERPEEVTDMQRSVKGEVIASTFDRPPSDHTQAAELAIERAKRLVEQGKDVVV
                     LLDSITRLGRAYNNASPASGRILSGGVDSTALYPPKRFLGAARNIEEGGSLTIIATAM
                     VETGSTGDTVIFEEFKGTGNAELKLDRKISERRVFPAVDVNPSGTRKDELLLSTDEFA
                     VVHKLRRVLSGLDPHQAIDLLMSQLRKTKNNYEFLVQVSKTAPGSMDND"
BASE COUNT         1014 a         1967 c         1927 g          894 t
ORIGIN      
        1 tggcgacctg ctgggcgtgg ccgccacggg cgcgtactgc tattccatgt cgagtcgtta
       61 caacctgatc ggccgccccg cggtggtggc cgtgcgcgac gggcaagccc gcctgattct
      121 gcgccgggag acggtcgacg atcttctcag tctggaggta acagaatgaa tggtgacgaa
      181 aagccgatcg gcgtagccgt attggggctc ggcaatgtcg gcagtgaagt ggtgcgcatc
      241 atcgaggaca gcgccaacga tctgaccgcc cgcatcgggg ctccgctcgt gctgcgcggc
      301 attggggtgc gccgcgtggc agacaaccgt ggcgtgccgg tcgagttgct caccgacaac
      361 gtcgaagagc tggtgtcccg tgacgacgtc gacgtcgtcg tcgaactgat ggggccggtc
      421 gagcccgcgc gcaaggccat cctgagtgcg ctggaacagg gcaagtcggt cgtcaccgcc
      481 aacaaggcgc tgatggcggt gtccacgggc gaactggcgc aagccgccga gcatgcccac
      541 gtggatctct acttcgaggc ggccgtggcc ggtgccatcc ccgtcatccg cccgctcacc
      601 cagtcgttgg ccggtgacac cgtgttgcgc gtcgccggaa tcgtcaatgg caccaccaac
      661 tacatccttt cggagatgga cagcaccggc gccgactacg ccacggcgct cgctgatgcc
      721 agcgccctgg gctatgcgga ggccgatccg accgccgatg tcgagggcta cgacgccgcg
      781 gccaaggccg cgatcctggc atcgatcgcc ttccacaccc gcgtgcacgc cgatgacgtc
      841 taccgcgaag gcatcaccaa ggtgactccc gccgacttcg cctcggcgcg ctcgctcgga
      901 tgcacgatca aactgctggc catctgcgaa cgcctcacca ccgatgaagg ccagcaacgg
      961 gtttcggcgc gcgtctaccc ggcgctggtg cccctcgacc atccgctggc cgcggtcaac
     1021 ggcgcattca atgcggtcgt ggtggaggcc gaggccgccg gtcggctgat gttctacggc
     1081 cagggcgcgg gcggtgcacc cacggcctcc gcagtaatgg gtgacgtcgt gatggccgcc
     1141 cgcaaccgcg tgcagggcgg ccgcgggccg cgcgagtcga agtacgccaa gttgccgatc
     1201 tcaccgatcg gcttcatccc cacccgttac tacgtgaaca tgaatgtcgc cgaccggccc
     1261 ggcgtgttgt ccgctgtggc agccgaattc ggcaagcgcg aagtcagcat cgccgaggtc
     1321 cgccaggaag gcatggtcga cgagggcggc caacggtgcg gcgcccgcat cgtcgtcgtc
     1381 acccaccagg ccaccgacgc cgcgttgtcg gaaaccgttg cggcgctggc cgacctcgat
     1441 gtggtgcaga gtgtcaacag cgtgcttcgc atggaaggaa ccacggaatg accgccccca
     1501 ggaccgcggt gcaccagccc tggccgggcc tcatcgccgc ctaccgcgac cggctgccgg
     1561 tccaggacaa ctggaccgcg atcacgctgc tcgagggtgg cacaccgctg gtcccagcgc
     1621 cgcggctgtc cgaatacacc ggctgcacag tgcatctcaa ggtcgagggg ctcaacccga
     1681 ccggctcgtt caaggaccgc ggcatgacga tggccgtcac cgaagccgta gcgcgcggtc
     1741 agcaggccgt gctctgcgcg tcgacgggca acacctctgc gtcggctgcg gcgtatgcgg
     1801 cacgggccgg catcacctgt gcggtgctga taccgcaggg caagatcgcg atgggcaagc
     1861 tcgcgcaggc ggtcatgcac ggcgccaaga tcatccagat tgacggcaat ttcgacgatt
     1921 gccttgagct ggcccgcaag ctcaccgccg actatccgac catctcgctc gtcaactccg
     1981 tcaacccgta ccgcatcgaa ggccagaaga ccgcggcgtt cgagatcgtc gacgcgctcg
     2041 gcagcgcgcc cgacgtgcac tcactgccgg tcggcaacgc gggcaacatc accgcgtatt
     2101 ggaagggcta taccgagtac caccgcgacg gcgtatccga tcggctgccc cgcatgctcg
     2161 gtacgcaggc cgccggcgcc gcgccactgg tgctgggcga gccggtcagc aatcccgaga
     2221 caattgccac cgccatccgg atcggatcgc cggcgtcgtg ggcgtccgca gtggaggcgc
     2281 agcagcagtc caacggccgc ttccttgccg ccaccgacga ggagatcctg gcggcatatc
     2341 acctggtcgc gcggaccgag ggcgtcttcg tcgagcccgc gtcggcggcc agcatcgccg
     2401 gcctgctgaa gtcgatcgag gacggctggg tgacgagggg gtcgaccgtg gtctgcacgg
     2461 tgaccggcaa cgggctcaag gatcccgaca ccgcgctcaa gggcatgccc gcagtcaccc
     2521 ccgtgcctgt cgatcccagc gctgtggtcg agaagctggg cttggtctag ccacgtgaat
     2581 accctgccgg ccgggctgac cgccaccgcc gtcgtggcgg cctcgagcgc caacctcggc
     2641 cccggtttcg acagcctggg cctcgcgctc agcctctacg acgaaatcgt cgtcgagaca
     2701 accgaatccg gccttgtcgt cgaagtggaa ggggaaggcg ccggccaggt tccgcaggac
     2761 tcgtcgcacc tagtgatccg ggccatcgag cacggcctac aggccgtcgg cgtcacggtg
     2821 cccgggttga acctgcgctg ccgcaacgat attccgcatt cacgcggcct cggctcgtcg
     2881 gccgccgcgg tcgtcggtgg cctcgcggcc gtcaacggtc ttgtgacaca agcgggttcg
     2941 acagagctca gtgaatcgga actgatccag ctcgccagcg agttcgaggg tcaccccgac
     3001 aacgcgtcgg ccgccgttct gggtggtgcg gtggtgtcgt ggactgagca cgagggcgtg
     3061 ctgcctcggt atgcggcggc gccacttcgg gtgcaccccg acatccacct gtttcccgcg
     3121 attccggagg agcgctcgtc gacggcggag acgcgcgtac tgctgcccga ccgggtcagc
     3181 cacaccgacg cgcggttcaa cctcagccgc gcggcgctgc tggtgctggc gctgactgag
     3241 cggccggatc tgctgatgga agcgactgag gacgtcctgc atcagccgca gcgcgcaccc
     3301 gccatgccgg catcggcgga atacctgcgg atacttcggc gttgtggtgt tgcggcagtg
     3361 ttatccgggg ccggacctgc agtcattgcg ttgagttccc aggcagaatt gcctgcagag
     3421 gccctggagt acggcaccgc caatgggttc accgtcagca agatggccgt tggtgagggc
     3481 gtccgatgga cgtccggcgt ggccgttcgg agctagcttt gcggaccaca tgagtcacac
     3541 gcgaatcagg gtgtttcttg cttccggctc cgatgcgggt tattctcgct gtcgtccagc
     3601 aatcgcagcg tctctgcctg cgcagacact aggacgacca cttccccttt aggtgttcaa
     3661 tcttgtggac tgacggttcg ccgcataccg gcgaaacccg gccatcaccg ttgcagaggc
     3721 aatggtggga ccgccgcatt cagcgcatca gctgagtgcc cggacctccg cgccgacttg
     3781 gttagcgggg gatcagaaag gaaatccgtg actgaaacgg acctcttcac ggctggtagc
     3841 ggcagcgaaa atgtcgagct gccgcaatcc gaatccgtga cttcagacaa ttcatcttcc
     3901 ccggacgact ccggagcaga cgcgacgaac ggcacgtcca ccgcggagac cgcgcccacg
     3961 gccgacgtcg cgtccggcga tcgtcccgcg gccctgtcga ccatggtgct gcccgaactg
     4021 cgggcgctcg ccaagcagat cggcgtcgag ggcgcgtccg gaatgcgcaa gagcgaactc
     4081 gtcgctgcca tccgcgaacg ccgcggcgag tccaacggcc gtccgaaaga ggcggccagc
     4141 agcgacgcaa ccgacagcgc agctcctgca gccgacacga ctgagcagcc cgccgaggag
     4201 caggccccgc cgcgccgcga gcgtcgcggc gcctctcgcg gcgccggcgc accgggcggc
     4261 ccggacgaga agaagggcga gaccaagcaa gagtccgccc agcagaacaa ggccgatacc
     4321 gaagccgcgc aggccccgaa acaggaccgc aaatccgacg gcggcgacca gcaaggcgat
     4381 cagggcggcc agaaccgcgg cggcaacgcc ggcgatgacg acggcgaggg ccgtggcggt
     4441 cgccgtggcc gtcgcttccg tgaccgcagg cgccgtgagc gcggcgatgg cggcggcggt
     4501 gaccgcgata ccgagctgcg tgaggacgac gtcgttcagc ccgtcgcggg catcctcgac
     4561 gtcctcgaca actacgcgtt cgtccggacc tccggctatc tggccggccc caacgacgtg
     4621 tacgtgtcca tgaacatggt gcgcaagaac ggcctgcgcc gcggtgacgc ggtgaccggc
     4681 gccgtccgcg tgcccaagga cggtgagcag cccaaccagc ggcagaagtt caacccgttg
     4741 gtccggctgg acaccgtcaa cggcaagccc gtcgaagagg ccaagaagcg gccagacttc
     4801 accaagctca ccccgctgta tcccaacgag cggctgcgcc tggaaaccac ctccgacaag
     4861 ctgaccaccc gcgtgatcga cctgatcatg ccggtcggca aggggcagcg tgcgctgatc
     4921 gtgtcgccgc cgaaggccgg taagaccacg atccttcagg acatcgccaa cgcgatcacc
     4981 aagaacaacc cggaatgcca tctgatggtg gtgctcgtcg acgagcgacc ggaagaggtc
     5041 accgacatgc agcgctcggt caagggtgag gtcatcgcct cgaccttcga ccgtccgccg
     5101 tcagaccaca cgcaggccgc cgagctggcg atcgagcggg ccaagcgcct ggtcgagcag
     5161 ggcaaggacg tcgtcgtgct actcgactcg atcacccgcc tgggccgcgc gtacaacaac
     5221 gcttcgcccg cgtcgggccg gatcctgtcc ggtggtgtcg actcgaccgc gctgtatccg
     5281 ccgaagcggt tcctcggcgc ggcccgcaac atcgaagagg gcggctcgct cacgatcatc
     5341 gccaccgcga tggtcgagac cggttcgacc ggcgacacgg tgatcttcga ggagttcaag
     5401 ggcaccggta acgccgagct caaactcgac cgcaagatct ccgagcgacg ggtgttcccg
     5461 gccgtcgacg tcaacccgtc cggcacccgc aaggacgagc tgctgctctc gacggacgag
     5521 ttcgcggtcg tacacaagct gcgccgcgtg ctgtccggtc tggatccgca tcaggccatc
     5581 gacctgctga tgagccagct gcgcaagacc aagaacaact acgagttcct ggtgcaggtc
     5641 tccaagaccg cgccgggctc gatggacaac gactaacgag cgatttcggc gcggttatcc
     5701 acgctcagcg ggctgttgca aaatgtggcg cccgattttg gtgatgtggc cgtgtcgtag
     5761 gtcggtgtga ttggcggctt ggctgaggat gtgttggtgg cc
//
LOCUS       DASRJA010000781         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3225853,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000781 DASRJA010000000
VERSION     DASRJA010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3225853"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VFC01_35290"
     CDS             <1..968
                     /locus_tag="VFC01_35290"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_215897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZN84921.1"
                     /translation="QQVGLVDVHPVGAVTMGLAGAQLTEMGMMAAGAGQVRMFSDDGI
                     CVHDPLIMRRALEYATGIGVLIAQHAEEPRLTVGAVAHEGPNAALLGLAGWPRAAEES
                     IVARDALLARDAAARVHICHASTAGTVEIVRWAKAQGISITAEVTPHHLLLDDSRLAT
                     YDGRNRVTPPLREASDAEALRQALADGVIDCVATDHAPHAEHEKFCEFAQARPGMLGL
                     QTALSVVVETMVRPGLLTWRDVARVMSENPARIVGLPDQGRPLEVGEPANLTVVDPDA
                     TWTVEGGALASRSDNTPYESMELPATVTLTLLRGKVTARDGKSPA"
     gene            965..1465
                     /locus_tag="VFC01_35295"
     CDS             965..1465
                     /locus_tag="VFC01_35295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011894597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HZN84922.1"
                     /translation="MNTGTLVTSLIMAAVLAVLIAFLIRQMLRGWLRRAQRQAQLIGT
                     LPPLPDTVGPALIPATKGLYVGSTLAPSWLDRIAVGDLGYRAKAVLTRYPEGIMLQRT
                     GAGPIWIPDDAIAAIRTEKGIAGKAMTHEGILAIRWRLPSGAEIDTGFRADNRDEYSK
                     WLEGVA"
     gene            1462..2589
                     /gene="carA"
                     /locus_tag="VFC01_35300"
     CDS             1462..2589
                     /gene="carA"
                     /locus_tag="VFC01_35300"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZN84923.1"
                     /translation="MTARAVLVLEDGRIFTGTPFGAVGQTLGEAVFSTGMSGYQETLT
                     DPSYHGQIVVATAPQIGNTGWNQEDAESRGDKIWVAGYAVRDPSPRASNWRATGALED
                     ELVRQEIVGIAGIDTRAVVRHLRSLGSMKAGVFSADALADPDELLTRVRSQPSMLGAN
                     LAGEVSTDASYVVEPDGPQRFTVAAIDLGIKTNTPRNFARRGIRSHVLPSTVEFAQIA
                     DLKPDGVFLSNGPGDPATADHIVAVTREVLGAGIPLFGICFGNQILGRALGRSTYKMV
                     FGHRGINVPVIDHATGRVAVTAQNHGFALEGEARERFDTDFGAAEVSHTCANDGVVEG
                     IKLVDGRAFSVQYHPEAAAGPHDAEYLFDQFVDLMAREVWS"
     gene            2704..>3228
                     /gene="carB"
                     /locus_tag="VFC01_35305"
     CDS             2704..>3228
                     /gene="carB"
                     /locus_tag="VFC01_35305"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177804.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HZN84924.1"
                     /translation="MPRRTDLNHVLVIGSGPIVIGQACEFDYSGTQACRVLRADGLQV
                     SLVNSNPATIMTDPEFADHTYVEPITPAFVERVIAQQAERGNKIDALLATLGGQTALN
                     TAVALYENGVLEKYGVELIGADFEAIQRGEDRQRFKDIVAKVGGESARSRVCFTMDEV
                     RETVEELGLPVVVRP"
BASE COUNT          533 a         1094 c         1104 g          497 t
ORIGIN      
        1 gccaacaggt ggggctcgtc gatgtgcatc ccgtcggcgc cgtcaccatg gggctggcgg
       61 gcgcccaact caccgagatg ggcatgatgg ccgccggcgc cggacaggtg cggatgttct
      121 ccgacgacgg catctgcgtg cacgacccgc tgatcatgcg ccgcgccctc gaatacgcca
      181 cgggcattgg tgtcctcatt gcccagcacg ccgaggagcc ccggctcacc gttggcgccg
      241 tcgcgcacga aggacccaac gccgcgctgc tcggcttggc cggctggccc cgcgccgccg
      301 aggaatccat cgtcgcccgc gacgcgctgc tggcccgcga cgccgcagcg cgggtgcaca
      361 tctgccacgc ttccaccgcc ggcaccgtcg agatcgtcag atgggctaag gcgcaaggta
      421 tttcgatcac tgcggaggtc actccgcatc acctgctgtt ggacgacagc cgactggcga
      481 cgtatgacgg ccgcaatcgt gtcaccccgc cgctgcgcga ggcgtcagac gccgaggcgc
      541 tgcgccaggc gctggccgac ggcgtcatcg actgtgtggc cactgaccac gcgccgcacg
      601 ccgagcatga gaagttttgc gaattcgcgc aggctcggcc cggcatgctc gggctgcaga
      661 ccgcgctgtc ggtggtggtc gagacgatgg tgcggccggg cctgctgacg tggcgcgacg
      721 tcgcccgcgt gatgagcgaa aaccccgccc gcatagtcgg tttgccggat cagggccgac
      781 ccctcgaggt cggcgagccc gccaatctga ccgtcgtcga tcccgacgcc acctggaccg
      841 tcgagggcgg cgctctggcc agccggtccg acaacacgcc gtatgaatcc atggagttgc
      901 ccgcgaccgt cacgctgacg ctgctgcgtg gcaaggtgac cgcgcgggac gggaagagcc
      961 cggcgtgaac accggaactc tcgtcacgtc gctgatcatg gccgcggtgc tcgcggtgct
     1021 gatcgcgttc ctgatccggc agatgctgcg cggctggctg cgccgcgccc agcgacaagc
     1081 gcaattgatt gggacgctgc cgccgctgcc ggacaccgtc ggtccggcgc tcattcccgc
     1141 gaccaagggc ctctatgtcg ggagcacgct ggcacccagc tggctggacc gcatcgcggt
     1201 cggtgacctc ggctaccgcg ccaaggctgt gctgacccgc tatcccgaag gaatcatgtt
     1261 gcagcgcacc ggcgcagggc ccatttggat acccgacgac gcgatcgccg cgatccgcac
     1321 cgagaagggc atcgcgggca aggcgatgac ccacgaaggc attttggcga tccgctggcg
     1381 actgccgtcg ggggccgaaa tcgacaccgg attccgcgcc gacaaccgcg acgagtactc
     1441 gaagtggctg gagggggtgg catgaccgcc agggcggtgc tggttctcga ggacggccgg
     1501 atcttcaccg gaaccccgtt cggcgccgtc gggcagacgc tcggcgaggc cgtgttctcg
     1561 accggcatgt ccggctacca agagacactg accgatccga gctatcacgg gcagatcgtg
     1621 gtcgccacgg cgccgcagat cggcaacacc ggctggaacc aggaggatgc cgaaagccgc
     1681 ggcgacaaga tctgggtggc cggctacgcg gtgcgcgacc cgtcgccgcg ggcctcgaac
     1741 tggcgtgcca ccggcgcgct cgaggacgaa ctcgtccgcc aagagatcgt cggaatcgcg
     1801 ggtatcgaca cccgcgccgt ggtgcgacac ctgcgcagcc tgggctcgat gaaggcgggg
     1861 gtgttcagtg cagatgcgtt ggccgacccg gacgagctgc tgaccagggt ccgcagccag
     1921 ccatcgatgc tcggcgccaa cctcgcgggt gaagtcagca ccgatgccag ctacgtcgtg
     1981 gaacccgatg ggccgcaacg gttcaccgtc gccgcgatcg acctcggcat caagacgaac
     2041 acgccgcgta acttcgccag gcgtggcatc cgaagtcacg tgttgccgtc gacggtcgag
     2101 ttcgcgcaga tcgccgacct caagccagac ggggtgttcc tgtccaacgg gccaggtgac
     2161 ccggcgaccg ccgatcatat tgtggccgtc acgcgtgagg tgctcggtgc cgggatcccg
     2221 ctgttcggca tctgcttcgg taaccagatc ctcggtcggg cgcttggccg ctcgacttac
     2281 aagatggtgt tcgggcaccg cggtatcaac gtgccggtca tcgaccacgc gacgggccgc
     2341 gtcgccgtta ccgcgcagaa ccacgggttc gcgctcgagg gcgaggcgcg ggagcggttc
     2401 gacacggact tcggcgccgc tgaggtcagc cacacctgtg ccaacgacgg tgtcgtcgaa
     2461 ggcatcaaac tcgttgacgg ccgcgccttt tcggtgcagt accacccgga ggcggcggcg
     2521 ggcccgcacg acgcggagta cctgttcgac cagttcgtcg acctgatggc acgggaggtg
     2581 tggtcgtgac cgcgccccgt agcaccgcga gcgtgcgctt ttgcacggat ttccgcggcg
     2641 tgtcgtgtgc aaacacgcac gtcctcagct tgcgcgtggg ccacgcggca gaagggacag
     2701 ctaatgccgc ggcggactga cctcaaccac gtgctggtga tcggctcagg gccgatcgtc
     2761 atcgggcagg cctgtgagtt cgactactcg ggaacccagg cgtgccgcgt gctgcgcgcc
     2821 gacgggctgc aggtgagcct ggtcaactcg aacccggcga ccatcatgac cgaccccgag
     2881 ttcgccgacc acacctatgt cgagccgatc actcctgcgt tcgtcgagcg ggtcatcgct
     2941 caacaagccg agcgcggcaa caagattgac gcgctcctgg ctacgctggg aggccagacc
     3001 gcgttgaaca ccgccgtggc gctctacgag aacggcgtgc tcgagaagta cggcgtcgaa
     3061 ctgatcggcg ccgacttcga ggccattcag cgcggcgagg accggcagcg gttcaaggac
     3121 atcgtcgcca aggtcggcgg cgaatccgcc cgctcccgcg tctgtttcac catggacgaa
     3181 gtgcgagaga cggtcgagga gctcgggctg ccagtcgtcg ttcgcccg
//
LOCUS       DASRJA010000782         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1253147,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000782 DASRJA010000000
VERSION     DASRJA010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253147"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VFC01_35310"
     CDS             complement(<1..246)
                     /locus_tag="VFC01_35310"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HZN84925.1"
                     /translation="MRFTETNLPGAYLVDLTLIEDERGFFARGWCTDEFARHGLVAAM
                     TQLNVGFSHRRGSLRGMHYQEHPHQEAKFVRCTRGAIF"
     gene            282..1658
                     /locus_tag="VFC01_35315"
     CDS             282..1658
                     /locus_tag="VFC01_35315"
                     /inference="COORDINATES: protein motif:HMM:NF044715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84926.1"
                     /translation="MRPSRRACPGRFDCREVVTMSVDTALRLREDTHNPIRVGIVGAG
                     STGRAIALQLATPVPGIRLAAIANRTPATAERALREAEIAGWARVDSAGAADEAIGRG
                     RTVLTDNAAVITSCPSIDVVIEVTGTVGPAARTVLDAFAHGKDVVLVNAELDSLLGPI
                     LKVKADEAGVVLTHTDGDEPGVAMTLLRYLRTLGLRPVAAGNIKGMVDYYRNPETQRA
                     FAEKTGQDVHKVTSFADATKLSMETTVLANATGFHCGRRGMHGPACTHVRELTERLPA
                     EQMLATGLVDYSVGAEPGTGAWVIVHESNALKQAQLSYYKMGNGPFYVFYTPYHLPHL
                     QIGSTIGRAVVHRDPTVAPLNGPVCEVVAMAKRDLGAGERLDGIGGFCTYGLIENTAT
                     ARAGRALPIGLAEGCVLRHDVPRDRMLSVDDVVEPQREPVIDQLWREQCERWPVAAAQ
                     PEVAAVNA"
     gene            complement(1726..>2771)
                     /locus_tag="VFC01_35320"
     CDS             complement(1726..>2771)
                     /locus_tag="VFC01_35320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84927.1"
                     /translation="WTDDNSRWRLWAGAAAIALAILCKITALYVGFVVLYLFVRRYGQ
                     RFVLKPTTWLVAAVMLVPAVLWYSHAYQMYLEDGNTFGIFAAGSLKFGTKSTLTDPFI
                     YKQTAIRVVFYHLTPLVFLGFCYGCYLSVIRRDRFVLAWLAGITVHALVAFRGLRYAG
                     HIGYLLTILPPCCLVAGLGFQTAFAELRQRPGYRSRPSVRVPLLAAFALLVVVNTAVM
                     YGRFNNRDLAFETALWEGKRLTGLKVGELTRPGSLIIVADHEMDGLTPKTWMTPPDVF
                     FFGDRRGWYFALSWATPERIEDVRAKGAEYFVVSANSLFQYVNQYSTLDAYLNQHYRK
                     IYGQEGIIYDLRR"
BASE COUNT          516 a          883 c          934 g          438 t
ORIGIN      
        1 gaagatggcc ccgcgcgtgc acctgacgaa tttcgcctcc tgatggggat gctcctggta
       61 gtgcatgccg cgcagactgc cacgcctgtg actgaagccg acgttcaact gggtcatggc
      121 ggcgaccagt ccatgccggg cgaactcgtc ggtgcaccag ccgcgcgcga aaaagccccg
      181 ctcgtcttcg atcagcgtga ggtcgaccag gtacgcgccg ggaagattgg tctcggtgaa
      241 tcgcatagtc ggttatcgcc ggttgatatt ctgtcacgca tgtgcgcccg tcgcggcgcg
      301 cttgccccgg tcggttcgat tgtagagagg tcgtcacgat gagcgtcgac accgcgcttc
      361 gcctgcgcga agacacccac aatcccatcc gtgtcggcat cgtcggcgcc ggttccaccg
      421 gccgtgcgat tgcgctgcag ctggcaacgc cggtgccggg cattcgcctt gcggcgatcg
      481 ccaaccgcac gccggcaacc gccgagcggg cgcttcgcga ggcggagatc gccggctggg
      541 cgcgggtcga ctcggcgggg gcagccgacg aggcgatcgg ccgaggtcgg acggttctca
      601 ccgacaacgc cgcggtgatt acctcgtgcc cctcgatcga cgtcgtcatc gaagtgacgg
      661 gcacggtcgg cccggcggcg cgcaccgtgc tggatgcctt cgcgcatggc aaggacgtcg
      721 tgctggtgaa cgccgagctc gattcgctgc tcgggccgat tctcaaggtg aaggcggacg
      781 aggccggtgt cgtgctgacg cacaccgacg gcgacgagcc cggcgtggcc atgacgctgc
      841 tgcggtacct acggacactc ggattgcgcc cggtcgccgc cggcaacatc aaggggatgg
      901 tcgactacta ccggaacccc gaaacccagc gggcattcgc cgagaagacc ggtcaggacg
      961 ttcacaaggt gacctcgttc gcggatgcca ccaagctgtc gatggagacc acggtgctgg
     1021 ccaacgcgac cggctttcac tgcggccgac gcggcatgca cggtcccgcg tgcacccacg
     1081 ttcgcgagct caccgagcgg ctgccggcgg aacagatgct cgccacgggc ctggtcgact
     1141 actcggtcgg cgccgagccc ggcaccggtg cgtgggtgat cgtgcacgaa tcgaacgcgc
     1201 tcaaacaggc acagctctcc tactacaaga tgggcaacgg cccgttctac gtgttctaca
     1261 cgccgtacca cctgccgcac ctgcagattg gctcgacgat cggccgggcg gtggttcacc
     1321 gcgatccgac ggtggcgccg ctgaacggcc cggtgtgcga ggtcgtcgcg atggcgaagc
     1381 gcgacctcgg ggccggtgag cggctcgatg gcatcggcgg gttctgcacc tacggcctga
     1441 tcgagaacac cgcgacggcg cgcgccggac gcgcgctgcc gatcgggttg gccgaaggct
     1501 gtgtcctgcg tcacgacgtg ccgcgagatc gcatgctgtc ggtcgacgat gtggtggagc
     1561 cgcagcggga gccggtaatc gatcagctgt ggcgcgagca gtgcgagcgg tggccggtgg
     1621 cggcggcgca gccggaagtg gccgcggtca acgcctgagc gtcctgtcgt ggcggtcgtg
     1681 gctcctggca cgggcgccac ggcttcgtca tcgactgaca cgacgtcagc ggcgcaaatc
     1741 gtagatgatg ccttcctgcc cgtagatctt ccggtagtgt tgattcaggt acgcgtcaag
     1801 ggtcgagtac tggttgacgt actgaaaaag cgaattcgcg gacaccacga agtattcagc
     1861 gcccttcgcg cgcacgtcct cgattctctc cggcgtcgcc catgacaggg cgaaatacca
     1921 gcctcgccgg tccccgaaga agaacacgtc cggtggcgtc atccacgtct tcggcgtgag
     1981 gccatccatc tcatgatcgg cgacgatgat gagcgaacca ggccgcgtga gttcaccgac
     2041 cttgagtccc gtcaggcgct ttccctccca gagggccgtc tcgaacgcca ggtcgcgatt
     2101 gttgaaccgg ccatacatca cggcggtatt cacgaccacg agaagggcga aggcggcaag
     2161 cagcggaacc cgcaccgacg ggcgtgagcg gtacccgggg cgttggcgca attctgcgaa
     2221 agcggtctgg aagcccagac ccgccacgag acagcagggg ggaaggatcg tcagcagata
     2281 gccgatatga ccggcgtacc gcaggcctcg gaacgccacg agggcatgga cggtaatgcc
     2341 tgcaagccac gccaagacga accgatcgcg gcgaatcacc gacaggtagc agccgtaaca
     2401 gaagccgagg aacacgagcg gcgtcaagtg gtagaaaacg acccgaatcg ccgtctgctt
     2461 gtagatgaac gggtccgtca acgtactctt cgtcccgaac ttgagcgagc ccgcggcgaa
     2521 gatgccgaac gtgttgccgt cttcgagata catctgatac gcatgggagt accagagaac
     2581 ggcggggacc agcatcacgg cagcgacaag ccaggtggtc ggcttcagca cgaatcgctg
     2641 accgtagcgc cggacgaaga ggtacagcac gacgaatccg acgtaaaggg cggtaatctt
     2701 gcaaaggatc gccagcgcga tcgccgctgc gccggcccac aggcgccacc gggaattgtc
     2761 gtcggtccaa c
//
LOCUS       DASRJA010000783         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2709497,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000783 DASRJA010000000
VERSION     DASRJA010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2709497"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFC01_35325"
     CDS             complement(<1..414)
                     /locus_tag="VFC01_35325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84928.1"
                     /translation="MHVAAQTLIEELGGFATTAQLLTVMTRQQLDVQVRKGGLVRVWR
                     GVYAASEPDLIGRLAALDLLMGQRAVPCMGTAAALYGFDLENTRAVHVLDPGVRMRPT
                     VGLMVHQRMGAPLRRVAGRLATAPAWTAVEVARQLH"
     gene            complement(565..2169)
                     /locus_tag="VFC01_35330"
     CDS             complement(565..2169)
                     /locus_tag="VFC01_35330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN84929.1"
                     /translation="MTDWDNDVDVVVLGSGGAGLTAALTAASNGASVEVYEKAATVGG
                     TTAVSGGIVWIPAHKRSADGELPVEDAMAYLRAQSLGFMDDELMETFVRTGAEMLDYV
                     EAHSELQFEIAEGFPDYKPELPGGRPSGGRSLNAKPFDLSRLGEWRDRITSFPADFSN
                     VGIDAETRARIHASVDNESGDYCVAGTALIAGLLRGLLDIGVVPRTDARAVELISDSG
                     QITGVRISLGGKDIRVRARRGVVLATGGFEWDAKLVEAYLRGPMRGAVSPPNNTGDGL
                     RMAMAHGADLANMGEAWWVPIVQLPGDTFQGHPRSRSVRLERTRPRSIIVNRAGKRFL
                     NEAGEYNSMAGPFQYLDPRDGYANDPAWIVFDSQHLKRYGFLGVLPGDEAPDWFSPSK
                     DLAELGAKTGIDADGLARTLAAWNDNVAHETDPDFGRGSSAYDGYWGDTSAATVAGQT
                     LGPIDIAPYYAVPVSVGAMGTKGGPRTDRDGRVLHVDGSVIPGLYAAGNAMAGATGKA
                     YGGAGGTLGPAMVFGYRAGLAVAARS"
     gene            complement(2182..>2596)
                     /locus_tag="VFC01_35335"
     CDS             complement(2182..>2596)
                     /locus_tag="VFC01_35335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZN84930.1"
                     /translation="TLRALADELGTTVSLLVAEGDQQVAIAVIVPSNVFYQLSFHEGS
                     RYPLDRGAAGIALLASMPPRPGERDLVPQARQQGWIITHGEVEPNTYGLAVPVKRRPP
                     SPPTCINLISHREDVVLSGRDAVINAANELSAILG"
BASE COUNT          452 a          903 c          793 g          448 t
ORIGIN      
        1 atgtaactgc cgggcgacct ccacagcagt ccacgcgggg gccgtcgcca atcgacccgc
       61 aacccgtcgc agcggcgcac ccattcgctg gtgcaccatc aagccgaccg tgggtcgcat
      121 tctcacgccg gggtcaagca catgaacagc gcgggtgttc tcgagatcga agccgtacaa
      181 cgcagcagcc gtgcccatgc acgggacagc acgctgtccc atgagcagat caagcgccgc
      241 cagtcggccg atcaggtctg gctcggaagc ggcatagacg cccctccaca cgcgaacgag
      301 accacccttt cgaacctgga catccagctg ctgacgtgtc atgaccgtca acagctgtgc
      361 ggttgtggcg aatccaccaa gctcttcgat tagcgtctgg gcagcgacat gcatctctcg
      421 aatcatcaac gccggtctcc caagacgtca gccacgacca ctgaacttgg ggatgaagcg
      481 cggactgtgg ataagtctca tcgctgagca gacgcaaact cgcacaaatt gcggctgatg
      541 agtgcgagtt tgcgtctgct cgcgctagga acgcgcggcc acggcgaggc ctgcgcggta
      601 gccgaacacc attgcggggc ccagggttcc gcctgcgcct ccgtacgcct tacccgtcgc
      661 gccggccatc gcgttacctg cggcatacag ccccgggatc accgagccgt ccacatgcag
      721 gacacgaccg tcgcggtcgg tgcgcggacc acccttggtg cccatcgcgc cgaccgacac
      781 gggaacggcg tagtacgggg caatatcgat gggacccagc gtctgacccg ccacggttgc
      841 ggcgctggtg tcgccccaat acccgtcgta agcgcttgag ccccggccga agtcgggatc
      901 tgtctcgtgt gcgacgttgt cgttccacgc cgccagtgtg cgtgcgaggc cgtcggcgtc
      961 gatgccggtc ttagcgccca attccgcgag atctttcgac ggcgagaacc agtccggcgc
     1021 ctcgtcgcct ggcagcacgc ccaggaagcc ataccgcttg aggtgctgcg agtcgaacac
     1081 gatccacgcg ggatcgttcg cgtacccgtc gcgtgggtcg aggtactgga acggtcccgc
     1141 catcgagttg tactcgcccg cttcgttgag gaaccgcttg cccgcccggt tgacgatgat
     1201 gctgcgggga cgggtccgtt caagccgcac actgcggctg cgcgggtgtc cctggaacgt
     1261 gtcgcccggc agctgcacaa tcggcaccca ccacgcctcg cccatgttcg ctaggtcggc
     1321 accgtgcgcc atcgccatgc gcagtccatc gccggtgtta ttcggcggcg acaccgcgcc
     1381 tcgcatcggt ccgcgcaggt aggcttcgac gagcttggcg tcccactcga accccccggt
     1441 cgccaacacg acgccacgcc tagcccgcac ccgaatgtcc ttgccgccca gactgatacg
     1501 cacaccggtg atctggcctg aatcggaaat cagctccacg gcccgggcgt ccgttcgcgg
     1561 gaccacgccg atgtcgagca gtcccctcag cagcccggcg atcaatgcag tgccggccac
     1621 gcagtagtca ccggactcgt tgtcgaccga cgcgtggatg cgtgcacggg tctctgcatc
     1681 gatgccgaca ttgctgaaat ctgccgggaa cgaggtgatc cggtctcgcc attcgcctaa
     1741 tcgcgacaga tcgaacggct tggcattcag cgaccgtccg cccgacggcc gtccgccggg
     1801 cagttccggc ttgtaatccg ggaacccctc ggcgatctcg aactgcagct cgctgtgcgc
     1861 ttcgacgtag tcgagcatct cggcgccggt ccgaacgaac gtctccatca actcgtcgtc
     1921 catgaacccg agtgactgcg cgcgcagata ggccatcgca tcctcgaccg gcaactcgcc
     1981 atcggcggag cgcttatgcg cgggaatcca tacgatgcca ccggagaccg cggtcgtgcc
     2041 accgaccgtc gccgccttct cgtaaacctc gaccgaggcg ccgttgctcg ccgcggtgag
     2101 cgcggccgtc aaacctgcgc cgccgcttcc caggaccact acgtcgacgt cgttgtccca
     2161 gtcggtcacg gttgtctccc ttcagccgag aatcgcggac aattcgttgg ccgcgttgat
     2221 gaccgcgtcc ctgccgctca acaccacatc ctcgcggtga gaaatgagat tgatgcatgt
     2281 cggcggagac ggcggtcgtc gcttcacggg caccgcaagt ccgtacgtgt tcggttccac
     2341 ttcgccgtgc gtgatgatcc aaccttgttg tcgcgcttgg ggaacgaggt cacgctcgcc
     2401 cgggcgcggc ggcatgctgg cgagcagagc gattccggcc gcgccgcggt ccagcggata
     2461 ccggctgccc tcgtgaaacg acagttgata gaagacgttg ctaggcacga tcaccgcgat
     2521 cgccacctgc tgatcgccct cggcgacaag gagtgacacc gtggtgccga gttcgtcggc
     2581 cagtgcccgc agtgtc
//
LOCUS       DASRJA010000784         4399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1253274,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000784 DASRJA010000000
VERSION     DASRJA010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253274"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VFC01_35340"
     CDS             <1..575
                     /locus_tag="VFC01_35340"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HZN84931.1"
                     /translation="TSSVVERASTHSGAVRLSGGYLVHSTQQTLDDTPLLKVWRLGSQ
                     TVTTIGPLGSPLGDIHDETLAWIGSDRIARLTPLAPFTARPRYLGNALGSASFAPGKG
                     QAWTPEFAISKALPSCTVTITSGTAVRRTMSCATTVGAARVTWNGRDSAGRLLPKGRY
                     TWTLTGADSDGSLLWWTGSANPIRGTVTNT"
     gene            complement(942..1778)
                     /locus_tag="VFC01_35345"
     CDS             complement(942..1778)
                     /locus_tag="VFC01_35345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZN84932.1"
                     /translation="MDEFEAHRSHLRAVAYRMLGSRSEADDAVQEAWLRLARADPNGI
                     QNTRGWLTTVVARVCLDMLRSRKARHEVPLDTPQPADSPDPEQEAMLADSIGLALLVV
                     LDTLTPAERLAFVLHDMFAVPFREIAPIVGRSAQATKMLASRARHRVGASAMPNTDRH
                     RQRAVVDAFLAASRNGDFAALLDLLDPDVTVRVDASAATPGTPTRLRGARAIARQALD
                     FSHRAKHAHTGLVNGLPAILVTPRGHLAMVMTFTINNGTIVAIDIVADPQHLGQLTIE
                     ST"
     gene            1851..2276
                     /locus_tag="VFC01_35350"
     CDS             1851..2276
                     /locus_tag="VFC01_35350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011600491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN84933.1"
                     /translation="MEPLVSTIEIARPPEEVFAFATDPLHFAEWQHDVVSVHMLEDSR
                     FTTTRRISGAERTMTQQITRNDPPRGWAAQGIDGPIRPHATITIEPVNNGARSRVTFA
                     LDFEGHGLGAPLVPLVRRQARKGAPNSYRNLKRLLESDQ"
     gene            complement(2820..3437)
                     /locus_tag="VFC01_35355"
     CDS             complement(2820..3437)
                     /locus_tag="VFC01_35355"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN84934.1"
                     /translation="MNHIDHLIYAAPDLENGVREIEERFGVRADGGGQHLGQGTHNKL
                     LALGPTAYLEIIAPDPTQPEPPDPRPYGVDGITHSGLVGWAIACDNIDDSLEAARAAG
                     YDPGEVIEGHRLTASGTMLRWRVTANARTAGVIPFLIRWGDTAHPAAAAPVGLMLESM
                     HIEHPDPESIASPLRALGADVDVRPGARVALVADISGPKGGGQLR"
     gene            complement(3586..3789)
                     /locus_tag="VFC01_35360"
     CDS             complement(3586..3789)
                     /locus_tag="VFC01_35360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84935.1"
                     /translation="MRTVAKHANRCLGIYATVSSPGTIVVGDEIVVRRPASPSRPTAM
                     TRVGARSLKRGVLRAANAMVPKE"
     gene            complement(3789..>4399)
                     /locus_tag="VFC01_35365"
     CDS             complement(3789..>4399)
                     /locus_tag="VFC01_35365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZN84936.1"
                     /translation="TTARRRPALLRCGARYAADPSGFAPEVVIVLPDGEEISSADPGV
                     HARLSELVGSKVRLEPIPPPSDKDAYRAPRETKADIRSHFGLAEDEPLPDLSVFPLRK
                     LAELSRYVTPVGSLFDAYPVHVITRASLAAMAALTPGSDFDVRRFRPNLSSTPNRATS
                     CPRTAGATPTSTCRTQVCTARSPRCVASCLPASSASSRQTSK"
BASE COUNT          708 a         1449 c         1560 g          682 t
ORIGIN      
        1 ccacgtcctc tgtcgtcgag cgcgccagca cgcactccgg tgccgtccgg ctcagcggcg
       61 ggtacctggt gcactcaacc cagcagacgc tcgacgacac cccgttgctc aaggtctggc
      121 ggctgggctc ccagaccgtc acgacgatcg gcccgctggg cagcccgctc ggcgacatcc
      181 acgacgagac gctcgcgtgg atcgggtccg acaggatcgc cagactgacg ccgctcgccc
      241 cgttcaccgc ccggccgcgc tacctcggca acgcgctcgg ctccgcgtcc ttcgcccccg
      301 gcaagggtca ggcgtggacg ccggagttcg cgatcagcaa ggccctgccg agctgcacgg
      361 tgaccatcac cagcggtacg gcggtgcgcc ggacgatgtc ctgtgcgacc accgtgggag
      421 cggcgcgggt cacgtggaac ggccgggact ccgccggccg cctgctgccc aagggccgct
      481 acacctggac cctgaccggt gccgactccg acggctcact gctgtggtgg accggctccg
      541 cgaaccccat ccgcggcacc gtcaccaaca cctgactcga tgcaccacgc gttagcggag
      601 atgcgtggcg tggaacgcct cgcgcacgtc gacccggtcc agcgaggccg ggtcgatgcc
      661 gagggcgccg aacagcggct tgcccgcgcc gagcagcacc gggcatcagg tggacgagga
      721 ctgggcagtt cgcgcatgtg ctggagcggg ccaggcagga cggcgacgac gccgcccagc
      781 gtgcgcaggc cgaggcggac ccggccgcga ccgttgtccg ggtgatcgac gcaccggcct
      841 ggagcgacct cggccagacg acgtcgagcg caatctcagc gcccgagagg ggcactgatc
      901 acggcagatc cgggcgttgg gtggcgacga caagttctgg gctacgtcga ttcgatcgtg
      961 agctggccga ggtgctgggg gtctgcaacg atgtcgatcg cgacgatcgt gccgttgttg
     1021 atggtgaagg tcatgaccat ggcgaggtgc ccgcgtggtg tcaccaggat cgccggaagg
     1081 ccgttgacga gtccggtgtg ggcgtgcttg gcccggtgcg agaaatccag cgcctgccgc
     1141 gcgattgcac gggcgccgcg gaggcgagtg ggcgtccctg gggttgctgc tgaggcgtcg
     1201 acacgcactg tgacgtcggg gtcgagcagg tcgagcagcg cggcgaagtc gccgttgcgt
     1261 gatgcggcga ggaaggcgtc gacgaccgcc cgctgccggt gtcgatcggt gttgggcatg
     1321 gccgaggcgc ccactcggtg gcgcgcgcgg ctggccaaca tcttggtcgc ctgggccgag
     1381 cggccgacga tcggcgcgat ctcgcggaag ggcacggcaa acatgtcgtg caggacgaag
     1441 gccagccgtt ccgcgggggt cagcgtgtcc agcacgacca gcagggcgag cccgatcgag
     1501 tcggccagca tcgcctcctg ctctgggtcg gggctgtccg cgggttgcgg tgtgtcaagg
     1561 ggaacctcat gccgcgcctt gcgcgagcgc agcatgtcca ggcacacgcg cgccacgacg
     1621 gtggtgagcc agcctcgggt gttttggatg ccattcgggt cggcgcgggc cagccgcagc
     1681 cacgcctctt ggaccgcgtc gtcggcttcg ctgcgcgagc cgagcatgcg gtaggccacc
     1741 gcgcgcaggt ggcttcggtg cgcctcgaac tcgtccaccg gttacctttc cgccccacac
     1801 tccgtcatag cactgacgag tgaaggcagg cagaggtaac aggaggaccg atggaaccgc
     1861 tggtttccac gatcgagatc gcgcgcccgc cggaggaggt ctttgccttc gcgaccgatc
     1921 cgctgcactt cgccgagtgg cagcacgatg tggtgagcgt ccatatgctc gaagattcgc
     1981 ggttcaccac gacccggcgg atcagcggcg ccgagcgcac gatgacccaa cagatcaccc
     2041 gcaacgaccc ccctcgcggc tgggccgccc aaggcatcga cggccctatc cgcccgcacg
     2101 ccacgatcac catcgagccg gtcaacaacg gtgcgcggtc acgagtgacc ttcgccctcg
     2161 acttcgaagg tcacggactc ggcgcacccc tggtaccgct ggtacgccgg caggcacgaa
     2221 agggtgctcc gaacagctac cggaacctca agaggcttct tgagagcgac cagtagtagg
     2281 gcaagcatcc ggaccccgca cgcaccgacg ccgagctagc ggagggcgcg cacacgggcc
     2341 aggtccgcag gattaccggc agaacccgga tgagcggacg tccgcgactc ggcatcagcg
     2401 aacgttcgcg cagcaccggt tgctcgacga gcggtgcccc aggcgactct cgcagcccag
     2461 cacggtgaac tgtcgcatga ccgcaggtga ccgaaccgat gagccctagg gtggtcatgg
     2521 tcggccggcg gacgcgccga cagcgcgtgg tgctgttcgt ggtgcgagat ggaggcaatt
     2581 cgtgcggatt gtggggcgtg gtgcggccac ccgcgttggt attggtgctg gtagagaccg
     2641 tatgaggcac tgtccttcct actcgtagtg agaaggtcct cggttcgatc cgacgggcgg
     2701 ctcacgacga aaccccagcg tcgcgatgcg attgagcagt tcctgaacgg acagcagcac
     2761 ccgctcatcg gcatgaccgc gcggacagcg gcagatgagt gcgtccatgg cgcgacgggc
     2821 taccggagtt ggccgccgcc ttttggcccg ctgatatcgg cgacgagtgc gacccgggcg
     2881 ccaggtctga cgtcgacgtc cgcgccgagg gcgcgaagcg gcgacgcgat cgactcgggg
     2941 tccgggtgtt cgatgtgcat cgattccagc atgaggccga ccggtgcggc tgcggccggg
     3001 tgcgccgtgt cgccccatct gatcagaaag gggatgacac cggctgtgcg ggcattcgcg
     3061 gtgacgcgcc agcgaagcat cgtccctgac gcggtgagtc ggtggccttc gatcacctcg
     3121 cccgggtcgt agccggccgc gcgcgccgcc tcgagcgaat catcgatgtt gtcgcacgcg
     3181 atcgcccagc cgaccagccc gctgtgcgtg atcccgtcga cgccgtacgg gcgcggatcc
     3241 ggcggctcgg gctgagtggg gtcaggagcg atgatctcga ggtacgcggt tggcccgagc
     3301 gccagcagct tgttgtgggt gccctgaccg aggtgttgtc cgccgccgtc ggctcggacg
     3361 ccgaatcgct cctcaatctc ccggacgcca ttctcgagat ccggcgccgc gtagatgagg
     3421 tgatcgatgt ggttcatagg ttcgaacgct agcgctcacg cggctgctat agcgcggcgc
     3481 acaaggcgtt ggcgatcggt ccacggatag tgcaaagcaa aatggctcga tcgtgtcggc
     3541 gcgcggccgg cgccgtcgac catgacgaca tgagttacgg tccgcctact ccttcggcac
     3601 catcgcgttg gccgcgcgga ggacgccgcg cttcaggctg cgcgcgccga ctcgggtcat
     3661 ggccgtaggg cgagacggcg aggcaggccg gcgtaccacg atttcgtcac caacgacgat
     3721 ggtgcccggg ctgctcaccg tcgcatagat tccgaggcag cggttcgcgt gctttgccac
     3781 ggtgcgcatc acttggatgt ctgtcgggag ctcgcgctgc tcgcgggtag gcatgacgca
     3841 acgcagcgtg gggatctcgc cgtgcagacc tgcgttcggc aggtcgaggt cggcgttgca
     3901 ccagctgttc tcgggcaact cgtcgcccgg ttcggtgtcg acgacaggtt gggccggaac
     3961 cggcgaacat cgaagtcgga tcccggcgtg agcgcggcca tcgctgccag actggcgcga
     4021 gtgatcacat gcaccggata agcgtcgaaa agcgaaccga ctggcgtgac gtagcgactc
     4081 aattcggcga gcttgcggag cggaaacacg ctcaagtcag gcagcggctc gtcctcggcg
     4141 agcccgaagt gcgagcggat gtcggccttg gtttctcgcg gcgcccggta tgcatcctta
     4201 tcggaaggag gaggtatcgg ctcgagccgg accttgctgc cgaccagctc cgaaagccgg
     4261 gcatgcaccc ccggatcggc gctcgagatc tcctcgccgt cgggcaacac gatgacgact
     4321 tccggggcga accccgacgg atcggccgcg tagcgggcgc cgcacctcag cagcgcaggc
     4381 cggcgtcgtg cggtcgtta
//
LOCUS       DASRJA010000785         6309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_325224,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000785 DASRJA010000000
VERSION     DASRJA010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325224"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1543)
                     /locus_tag="VFC01_35370"
     CDS             complement(155..1543)
                     /locus_tag="VFC01_35370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HZN84937.1"
                     /translation="MRCLPRCSSANRHRAPSSSFLKPGVPSMRSRPSPTRRSIRRNIL
                     AGLIVVIILAGGVGGWAATAQISGALIAPGSLVVDSNVKKVQHPTGGVVGEVRARDGD
                     RVKAGDVVVRLDETITRANLAIVTKGLTELYARKARLAAERDGAGSIPMPKELANRAN
                     DPDVAEALSSERKLFDLRHTSRSGLKAQLAQRVAQLNEEITGLTTQQKAKAQEVVLIN
                     RELVGVRDLWEKKLIPITRLTQLEREATRLDGERGQLIAAAAQARGKVTETELQIIQI
                     DQDLSSEVAKEMREIDGKIGEFIERKVAAEDQLKRIDIRAPQDGYVFQSTVHTVGGVI
                     TAGEAIMMIVPEADNLMVEAKVDPKDIDRLQIGQPVVLRFVTFNQRTTPELNGTVTLI
                     AADASNDQRSGLSYFTIRIGMTAQEIARLGEVKLGPGMPVEAFVQTGDRTMFSYLMKP
                     LHDQLMRAFRER"
     gene            complement(1464..3224)
                     /locus_tag="VFC01_35375"
     CDS             complement(1464..3224)
                     /locus_tag="VFC01_35375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HZN84938.1"
                     /translation="MSRSTTKSPTTRDTPAAELAAALASCRGAFVATGLISGMSNVLM
                     LTGAIFMLEVYDRVLPSRSVPTLIGLAMLAGGLYVALGLLDLIRGRILTRIGISLDEA
                     LSERIYQTIVRLPSIVGLRNDGAGPMRDLDSVRSFLGSLGPVALFDLPWMPVYLGLCF
                     AFHFWIGMTATVGALILVMVTVLTEWFSRKPTMTATELAQSRNAMIESSRRNSEALVT
                     MGMVPHLTGRWSRVNQEFLASNRRASDAAGGFGAISKVLRLMLQSAVLGVGAYLVIYQ
                     QASAGIIIAGAIISARALAPVDIAIAHWRGWVAARQSWDRLKKLLATIPPDSEPMPLR
                     APQMSLVVENASVAPPGTRKPAVRDLSLKLQRGSGLGVIGPSGSGKSSLARMLVGYWA
                     PANGKVCLDGASLDQWAPDILGRHIGYLPQHVELLNGNVAQNISRFEPDADPEAIRVA
                     AETAGAHQMIVSLEDGYSRQIGERGSALSAGQAQRIALARALYRDPFLVVLDEPNSNL
                     DAEGEAALTQAILSIRARGGIVIVVAHRPSAIAGVDLVLAMKEGRAVAFGPKDEVLAK
                     VLQREPSPRPLKLVSEAGSA"
     gene            complement(3293..>6309)
                     /locus_tag="VFC01_35380"
     CDS             complement(3293..>6309)
                     /locus_tag="VFC01_35380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84939.1"
                     /translation="TVTLNTATGAYTVTQNAPIHHDLLPAGENNQAFTLTYRVTDGDG
                     DTVDGTLGINVDDDTPSGLTPDALNVDNIGIAGTSDQITAALNFSVGADGVGSVVFNV
                     PGVPVGVTSGITVAVTDLDGHLLFLGTQQLYLYYGGAGGTDTTILVAKTLTGTVGFTI
                     DINPLTGTYIFNPEGVISNGTAVTATNLTGVGGANVPFKLLIDIGGTNPTGQDVAMTT
                     ANGATINSDADDIGISQAQTFETGEAIRFDLVNGLKLDAASGFAYDGTHNETVRWKQT
                     IQMTGNESQRANIVVSAIVADSDNTFFGDSAGETFINLSTANIKIYDATGTLIPTANY
                     GALGINLTDQGNSILIEGLRDDWKYEIVTDDSHKFSAVQVEAATGTDNFSLGFFTYGV
                     DNPGTPIALSYDIVGTDVDGDSTTGTIDITLHPHVVSAPAGVAGEPINLGLTAPSAED
                     GALVTVAIADVPSGWTLNGGTLLDDGTWTVQTTAPRSLAITSPAAFAGAMLLNVTATW
                     AQVDGSTAKITIADNVEVFAPDAPIFAWSGDDHLTGSSGDDLFVFAQPIGHDVIYNFD
                     AAHDQVDLIAFAGMTSYADVQANLANDASGQAVLTLGDGMTITFDGVDAAALGASNFA
                     FNQEPVTNNPDTMVVSDGAMLPLGGIVNNTGTIALDSDGAETKLELIQHGITLQGGGQ
                     FTLSDSGGNVILGTDPSVTLTNVDNTISGAGQLGGGQMTLVNHGTIVATGSNALIIDT
                     GANAVINSGTLEAVGSGGLVIHADVENSGLLWANGGNLTVIGDVTGDGSAKIDGIATM
                     EFGAAVNQDITFDVSAAGTVKLDDADAFAGVIAGFDGNDQFDLSDVDFGAGLSLTYTA
                     NQGGTGGTLTVSDGTDTANIHLVGPYSAGGFQTAADIGTGTLVTYMPSEGVGKNVVVG
                     GVGTDDILSGGAGADTFQFAPSFGHDTIVNFEPGVDKIDFDQAIFATVDDILAHTQQV
                     GSDTVITVDQASSVTLTNVDMSTLSASDFIHH"
BASE COUNT         1095 a         2010 c         1980 g         1224 t
ORIGIN      
        1 cccagtgtgc cctgcaggaa ggagaaatcg cgaacgcaac gttcttgggc cgcggcttcg
       61 gcggctcatt gaggacatgc gtcaggagtg gcggagtatc gaccagcgga tgcggcgagc
      121 cgctgcgcac tttgcccgag agcactctgg cggctcacct ctcccggaac gcccgcatca
      181 gctggtcgtg caggggcttc atcagatagg agaacatcgt ccggtcgccg gtctgcacga
      241 acgcctcgac cggcatgccg ggcccgagct tgacttcgcc cagccgggca atctcctggg
      301 cggtcatccc gatccggatg gtgaagtagc tcaggcccga gcgctggtcg ttcgacgcgt
      361 cggccgcgat cagggtgacc gtgccgttga gctcgggcgt ggtccgctgg ttgaaggtca
      421 cgaagcgcag caccaccggc tggccgatct gcaggcggtc gatgtccttg gggtcgacct
      481 tcgcttcgac catcaggttg tccgcctcgg gcacgatcat catgatcgcc tcgccggccg
      541 tgatcacacc gccgactgtg tggaccgtcg actggaacac gtagccgtcc tgcggcgcgc
      601 ggatgtcgat gcgcttgagc tggtcctcag ccgcaacctt gcgctcgatg aactcgccga
      661 ttttgccgtc gatctcgcgc atctccttgg ccacctcgct gctgaggtcc tggtcgatct
      721 ggatgatctg cagctcggtc tcggtgacct tacccctggc ctgggccgcg gccgcgatta
      781 gctggccgcg ttcgccatcg agccgggttg cctcgcgctc gagttgggtg aggcgggtga
      841 ttgggatgag cttcttctcc cacaggtccc tgacgcctac gagctcgcga ttgatcagca
      901 cgacctcctg tgccttggct ttctgctgcg tcgtgaggcc ggtgatctcc tcgttgagct
      961 gggcgacgcg ctgggcgagc tgggccttca ggccgctgcg cgacgtgtgc cgcaagtcga
     1021 agagcttgcg ctcgctcgaa agcgcctcgg cgacatccgg atcgttggcg cgattggcga
     1081 gctccttggg catcgggatc gatcctgccc cgtcgcgctc cgcggcgagc cgggccttgc
     1141 gggcgtagag ctcggtcagg cctttggtga cgatggcgag gttggctcgc gtgatcgtct
     1201 cgtcgaggcg caccacgaca tcgcctgcct tgacgcggtc gccgtcgcgc gcgcgcacct
     1261 cgccgacgac gccgccggtc ggatgctgca cctttttcac gttcgagtcg acgacgaggg
     1321 agccgggcgc gatgagcgcg ccggaaatct gcgcggtggc ggcccaccca ccgacgccgc
     1381 cggcgagaat aatcacaact attagaccgg cgagaatgtt gcgccgaatg gatcgtcgcg
     1441 ttggtgaagg ccgagatcgc atgctaggca ctcccggctt cagaaacgag cttgaggggg
     1501 cgcggtgacg gttcgcgctg gagcaccttg gcaagcacct catccttcgg gccgaaggca
     1561 accgcgcgcc cttccttcat tgccaggacg agatccaccc cggcgatggc gcttgggcgg
     1621 tgtgcaacca cgatcacgat gccgccgcgg gcgcggatgc tgaggatggc ttgggtcaac
     1681 gccgcctcgc cctcggcgtc aagattggaa ttcggctcat cgagaaccac caggaacggg
     1741 tcgcgataga gcgcgcgggc aagcgctatg cgctgtgctt gccccgccga cagcgccgaa
     1801 ccacgctcgc cgatctgccg gctgtatcca tcctccagag aaacgatcat ctgatgggcg
     1861 ccggcggtct ccgccgccac ccgtatcgcc tcgggatccg catcgggttc gaaccggctg
     1921 atgttttgcg ccacattgcc gttgagcagt tcgacgtgtt gcggcagata gccgatatgg
     1981 cgtcccagaa tatccggagc ccactgatcc agtgacgcgc cgtcgaggca aaccttgccg
     2041 ttcgcaggcg cccaatatcc gaccagcatt cgcgcgagcg acgacttgcc agaaccgctc
     2101 ggcccgatta cgccgaggcc acttccgcgc tgcagcttca acgacaggtc gcgcacggcg
     2161 ggtttgcgcg tgcccggcgg tgcaacactg gcgttttcga ccactaggct catctgcggc
     2221 gcccgcagcg gcatcggctc gctgtctggc gggatcgtcg ccagcaattt cttcagtcga
     2281 tcccaacttt gccgcgccgc cacccagcct cgccaatggg caatcgcaat atcgaccggt
     2341 gccaacgcgc gagcactgat gatcgcgccc gcgatgataa ttcctgccga cgcttgctga
     2401 tagatgacaa gataggcgcc caccccgagc acggcggatt gcagcatcag ccgcagcacc
     2461 ttcgagattg cgccgaatcc cccggccgcg tcactcgcac gccggttgct cgcaagaaat
     2521 tcctgattga ccctgctcca gcgcccggtt agatggggaa ccatgcccat ggtgaccagc
     2581 gcctcggaat tgcgccggct cgactcgatc atcgcgttgc gtgactgcgc cagctcggtc
     2641 gccgtcattg tcggcttgcg cgagaaccat tcggtcaaga cggtcaccat gaccaaaatc
     2701 aatgctccga ccgtcgcagt catgccgatc cagaaatgga aggcgaagca caagccgaga
     2761 tagaccggca tccaaggcag atcgaatagc gccacaggcc caaggctgcc cagaaatgac
     2821 cggacgctgt ccaggtctcg catcggcccg gcgccatcgt tgcgcagccc gacgatcgac
     2881 ggcaggcgca cgatcgtctg ataaattcgt tcgctcagcg cctcgtcgag gctaatgccg
     2941 atgcgggtga ggatgcgccc tcggatcagg tcgagcagcc cgagcgccac atagaggccg
     3001 cctgcgagca tggcgagccc aatcaacgtc ggcacgctgc ggctcggcag cacccgatcg
     3061 tagacctcga gcatgaagat cgcgccagtg agcatgagga cattgctcat cccgctgatc
     3121 aggccggtgg cgacgaacgc gccgcggcag gatgccaggg cggcggccag ctcggccgca
     3181 ggggtgtcgc gcgtagtcgg agacttggtg gtggaacggc tcataagcca accctatact
     3241 gaagcatcgt gaccaaagcc cgacgaattc tacggaccgc aactcccgcg gatcagtggt
     3301 ggatgaaatc gcttgccgac aatgtactca tgtccacgtt cgtcaaggtg acactgctgg
     3361 cttggtcaac ggtgatgacc gtatcgctgc ccacctgttg cgtgtgagcc aatatgtcat
     3421 cgacagttgc gaaaatcgcc tgatcaaaat ctattttgtc aacgccaggc tcgaaattaa
     3481 cgatcgtgtc gtgaccgaag ctgggtgcaa actggaaggt gtcggcaccg gctccgcccg
     3541 acaagatgtc gtcggtgccc acaccgccga caaccacatt cttccccact ccctcggacg
     3601 gcatgtacgt gacgagtgtc cctgtgccga tgtctgccgc ggtttggaac cccccggctg
     3661 aatacgggcc gacgagatga atgttggccg tatccgtgcc gtcactcacg gtcagtgtgc
     3721 cgccagtccc gccctggttt gccgtatagg tgagcgacag cccggcccca aagtcgacgt
     3781 cggagagatc gaattgatca ttgccgtcaa agccggcgat gacgccggcg aatgcgtccg
     3841 cgtcgtcgag cttcaccgtg cccgccgcgc tgacgtcgaa cgtgatgtct tgattgaccg
     3901 cagcgccaaa ctccatcgtg gcaataccgt caatctttgc ggacccatcg ccagtcacgt
     3961 cgccgatcac agtcaggttg ccgccgttcg cccaaagcag cccagaattc tccacatcag
     4021 cgtgtatcac cagtccgccg ctgccgaccg cttccagcgt gcctgagttg atcacggcgt
     4081 tggcgccggt atcgataatg agcgcgttgc ttcccgtggc gacgatggtg ccgtggttga
     4141 caagcgtcat ctgcccgccg ccaagttggc cagcgccaga gatggtatta tccacgttgg
     4201 tcaaagtgac gctcggatcg gtccccaaga tcacgttccc gccgctatcg gagagcgtga
     4261 actggccgcc gccttgcagc gtgatgccgt gctgaatgag ctcgagcttg gtctcagccc
     4321 cgtccgaatc gagcgcgatc gtgccggtgt tgttgacaat gccgccgagc ggcaacatgg
     4381 cgccgtcgct caccaccatg gtgtcggggt tgttggtgac cggctcctga ttgaacgcga
     4441 aattgctcgc gccaagcgcg gccgcatcga caccatcgaa ggtgatggtc atgccgtcgc
     4501 ccagcgtgag cacggcctgg ccgctggcgt cgttcgcgag attggcctgc acgtcggcat
     4561 agcttgtcat gccggcaaag gcgatcagat cgacctgatc gtgcgcggcg tcgaaattgt
     4621 agatgacgtc gtgcccgatg ggttgggcga acacgaacag gtcgtctccg ctcgagccgg
     4681 tcagatggtc gtcgcccgac caggcaaaga tcggcgcgtc tggcgcgaag acctccacgt
     4741 tgtcggcaat cgttattttt gcggtgctgc cgtccacctg cgcccaggtc gcggtcacgt
     4801 tgagcaacat cgcacccgcg aaggccgctg gcgacgtgat cgccagcgat cgcggggcgg
     4861 tcgtctgcac cgtccaggtg ccgtcatcga gcagcgtgcc gccgttgagg gtccagccgg
     4921 agggcacgtc tgcgatcgcc accgtgacaa gggcgccgtc ctctgccgac ggcgccgtca
     4981 ggccgaggtt gatcggctcg ccggccaccc cagcgggcgc tgacaccacg tgcggatgga
     5041 gggttatgtc gatggttccg gttgtggaat caccgtcgac gtcggtgccc acaatgtcgt
     5101 agctgagtgc gatgggcgtg cccgggttgt cgaccccata ggtgaagaag cccaagctga
     5161 agttatccgt accagtggcc gcttccacct gaacggcgct gaacttatgc gaatcgtcgg
     5221 tgacgatctc gtacttccag tcgtcgcgca gtccctcgat gaggatggag ttgccctggt
     5281 cggtaagatt aataccaagg gcaccgtagt ttgcagttgg gatcagcgtg ccggtcgcgt
     5341 cgtagatttt gatgttggct gtactgagat tgatgaaagt ttcgcccgcg ctgtcaccga
     5401 agaaggtatt gtccgagtcg gccacgatcg cggatacgac gatattggcc ctctggctct
     5461 cgttcccggt catctgtatc gtctgtttcc accgcaccgt ctcattgtgc gtaccgtcgt
     5521 acgcgaatcc cgaggctgca tccagtttca atccgttgac cagatcgaag cggatggctt
     5581 cgccggtttc gaaggtttgt gcctggctga tgccgatatc gtccgcatcg ctgttgattg
     5641 ttgccccgtt cgcggtcgtc atcgccacgt cctgccccgt cggattcgtg ccgccgatat
     5701 cgatcagcag cttaaacggg acgttggcgc cgcccacacc ggtgaggttg gtcgccgtca
     5761 ccgccgtgcc gttggagatg acgccttcgg ggttaaaaat gtaggtgccg gtcagcggat
     5821 tgatgtcgat ggtaaagccg actgtgcccg tcagcgtttt tgccaccaga attgtggtat
     5881 ccgtcccgcc ggcgccgccg taatagagat agagctgctg ggtgccgagg aacagcagat
     5941 gcccgtcaag gtcggttacc gcgacggtga tgccgctggt tacccccacc ggcactcccg
     6001 gcacgttgaa cacaaccgaa ccgaccccgt cagctcccac actgaaattg agcgcggccg
     6061 ttatttgatc cgacgtgccg gcaatgccga tgttgtccac atttagggcg tccggggtca
     6121 gcccgctcgg cgtgtcgtcg tcaacgttga tgccgagcgt gccatccacc gtgtcgccgt
     6181 cgccgtccgt cacccggtag gtcagtgtga acgcctggtt gttctcgccg gccggcagca
     6241 gatcatggtg gatcggcgcg ttctgcgtca ccgtataggc gccggtcgcc gtgttgagcg
     6301 tcaccgtca
//
LOCUS       DASRJA010000786         8009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2666798,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000786 DASRJA010000000
VERSION     DASRJA010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2666798"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..835)
                     /locus_tag="VFC01_35385"
     CDS             complement(134..835)
                     /locus_tag="VFC01_35385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN84940.1"
                     /translation="MTESSTSAAPNGAPGSQTLARGLAALQLVAASRTGLTVQQVADD
                     IGVHRTIAYRLLTTLAQFRMVAKGEDGRYRSAAALAVLGASFDNNVRQLSVPTLRALA
                     DELGTTVSLLVAEGDQQVAIAVIVPSNVFYQLSFHEGSRYPLDRGAAGIALLASMPPR
                     PGERDLVPQARQQGWVITHGEVEPNTYGLAVPVRRRPPSPPTCINLISHREDVVMSGR
                     EAVIKAADELSAILN"
     gene            968..1831
                     /locus_tag="VFC01_35390"
     CDS             968..1831
                     /locus_tag="VFC01_35390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011561168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN84941.1"
                     /translation="MGEYESIWSDLQGVPFSQGYLDAGGVRTRYLHAGDPGKPALVFL
                     HGSGGHAEAYVRNLEAHAEHFSTWSIDMLGHGYTDKPGHPLEVRHYVDHLMAFLYAIG
                     AERAHISGESLGGWVAARAAVDHSDRIDRLVLNTAGGSQADPEVMKRIITLSMAAAEN
                     PAWETVQARIKWLMADKSKDYDDIVASRQRVYRQPGFVAAMKDIMVLQDPEIRARNLL
                     GPAEYGAITAPTLVVWTSDDPTADVAEGRRIASMIPGARFEVMPGCGHWPQYEDPKTF
                     NALHLDFLLGR"
     gene            1834..3510
                     /locus_tag="VFC01_35395"
     CDS             1834..3510
                     /locus_tag="VFC01_35395"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HZN84942.1"
                     /translation="MSNQADVVIVGAGPVGLTLANILGLQGVRTLVVDERPSVIDYPR
                     GVGLDDEAIRTFQAIGLVDRILPHTVPNQILRFFDAKRQLLAEMAPPDARFGWPKRNG
                     FVQPMVDAELLAGLDRFDHVVVVWNRQMESCTETDDGVTVEFADGHEAVHARYVVGCD
                     GGRSATRRLMGVSFDGTTSSTRWLVIDCANDPLGHPNSEVGADPARPYVSISIAHGIR
                     RFEFMIHGDESDEEAEDPAFVRRMLSQLVPHPERVDIIRHRVYTHHSRIAGAFRKGRL
                     MLAGDAAHLMPVWQGQGYNSGIRDAANLGWKLAAVVNGDAGDALLDTYDVERRKHARA
                     MIDLSTLVGRVISPTNRRVAALRDRVIHAASVVPTLKRYILEMRFKPMPRYEQGAVFH
                     AEPRSEASPTGTLFIQPRIDTREAQNVLLDDVLGPGFAVLCWSNNPRAFLGDEAFDRW
                     KALGATFVAARPMTQLHWTGHDDPDVVIVGDRTGALKAWFDIHTDSVLFLRPDRCIAG
                     ACIAQRAPELSASLFNVLHLTQGGGNGATRPLLYVAQPTTESSGTVAGSP"
     gene            3458..4378
                     /locus_tag="VFC01_35400"
     CDS             3458..4378
                     /locus_tag="VFC01_35400"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HZN84943.1"
                     /translation="MSHSPLLNLPGPSQDLLDDIESALAKARDFVTAYDPELVVIFSP
                     DHYNGFFYRTMPPFCIGTAAQGVGDYGTHAGPLDVPSDVATDCATAVLESGIDVAISA
                     SMDVDHGTVQPLQKLFGDATTRPVIPIFINSVATPLGPMRRVRALGAAVGTYLASLGK
                     RVLVVGSGGLSHDPPVPTLATAPPAALGRIVHGNPMTPEQRQARQVAVMEAAREFAGG
                     QGPLQPLNPDWDHAFLELLDSNRLSEVDAWSNSWIEQEAGHSAHEIRTWVAAFAAMAA
                     HGRYRTEQRFYRAAPELIAGFAIRTAVLDV"
     gene            4371..6101
                     /locus_tag="VFC01_35405"
     CDS             4371..6101
                     /locus_tag="VFC01_35405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZN84944.1"
                     /translation="MSDFDHTVDVLVVGSGGGGMTAALAAHTSGLDTLIVEKSPQFGG
                     STALSGGGIWVPGAPSQRKKGYVPDPDAVFQYLKQITGGLVSDARLRQYVDTAPVMME
                     FLENLSPWFEFVWKPGYADYYPELPGGSELGSTINVPAIDLRKLGDEEQNLLHPLALA
                     PKGIWFAPKDLRLFYQVRQNWRGKAVLLKLIWRMFRARVFGDRMAAIGQSLAARLRLA
                     LKQQDIPLWLNSPMTELITDIDGTVSGAVVERDGKQQRIQARRGVILASGGFDHDMTW
                     RREHLPVLEKDWSFGNPAAMGDGIRAGEKVGGSTDLLDEAWWFPAMCWPDGRLQFMLN
                     ERMMPSQFVVNGDGKRFINEAAPYMDFAHAMIDGHRSGITHIPCWLITDIQSFHRYVV
                     GGHLPIPKVPFAPVPTGWKVPKAWLDSGIAKEANSFEELAAKIQVPAANLRATAERFN
                     ELAHRGHDDDFNRGDSAYDNYYGDPTLANPNLYPLGKPPYYAFQIILGDLGTSGGLRT
                     DEFARVLRDDDSVVKGLYAVGNASAAVMGRSYAGAGATIGPAMTFGYVAAKHIAHQQS
                     DSRSQSEVTT"
     gene            6098..7429
                     /locus_tag="VFC01_35410"
     CDS             6098..7429
                     /locus_tag="VFC01_35410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZN84945.1"
                     /translation="MKISLFYEFPLPRPWTPDDEHKLFQDGLDEVEAADKAGFSTVWL
                     TEHHFLEEYCHSTAPEIFLAAASQRTKNIRLGFGIMHLPPAVNHPARVAERISTLDLL
                     SNGRVEFGTGESSSVGELGGFGIDPADKRKMWEEALEVSIRCMIEEPFTGFKGEHVEM
                     PPRNVVPKPLQKPHPPVWVACTRPASVSMAAQKGLGALSFAYTGPGPLTERVNGYYKE
                     FEESAVPVTPQMNPNILAIGGDLSMMVAPTDEQAIERLGRGGGFFAFGIMHYYMTGMH
                     TPGRTGVWKRHLEEIEKDPTIVYGPDRGPIGSPATVREFLRGYEESGVDELILLLTPR
                     RHEETMESIELMGREVLPEFIERHEKAAAEKAKRMEPILEKAEARRPKSNAPEFDETY
                     AFGGLPTGRENYTANEVSLAMEEMNAGIEAAAAKLKSGEGWTSRNPDADKK"
     gene            7433..>8009
                     /locus_tag="VFC01_35415"
     CDS             7433..>8009
                     /locus_tag="VFC01_35415"
                     /EC_number="1.1.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009975001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl-alcohol dehydrogenase"
                     /protein_id="HZN84946.1"
                     /translation="MGQIDELWRYDGRRVVVTGCASGIGEHVARQLTDLGAEVVGLDV
                     RRPALQLKEFHEVDLSDPASIDQAAATIGGQVDALFNIAGVSSGIGDPLRVVTINFLG
                     TRQFTEALVPSMPPGSAIANVSSLAASGYRENASVTAGLLDTVTMAEGIEWCQRNPAP
                     VADGAGYRLSKEAIILYGMANVATLGAKGIRI"
BASE COUNT         1443 a         2656 c         2552 g         1358 t
ORIGIN      
        1 cttcccaaca cgaccacatc gacgtcgttg acccagtccg tcaccgcagt aacctctccc
       61 tacgcgccga atgtgagctt gtgctcgaaa aaactcgaga atttcgcaca taagctcacg
      121 ttggaggccc ccttcagttc aggattgccg atagttcgtc ggccgccttg atgaccgcct
      181 ccctgccgct catgaccacg tcctcgcgat gggagatgag gttgatgcag gtcggcgggg
      241 acggcggccg tcgccgcacg ggaacagcaa ggccgtacgt gttcggctcg acttcgccgt
      301 gcgtgatgac ccaaccttgt tggcgagctt ggggaaccag gtcacgctct cccggacgcg
      361 gcggcatgct ggcgagcaga gcgattccgg ccgctccgcg gtccaatgga tatcggctgc
      421 cttcgtgaaa ggacagttga tagaagacgt tgctaggcac gatcaccgcg atcgccacct
      481 gctgatcgcc ctcggcgaca aggagtgaca ccgtggtgcc gagttcgtcg gccagtgccc
      541 gcagtgtcgg cacgctgagc tggcggacgt tgttgtcgaa tgacgccccc aagacagcga
      601 gtgccgcggc cgaccggtat cggccgtcct cgcccttggc gaccatccgg aactgagcca
      661 gtgtcgtgag caggcggtac gcgatggtcc ggtgaacgcc gatgtcgtcg gcgacctgct
      721 ggactgtcag cccggttcgg gaagcggcaa ccagttgcag cgccgccaaa cccctggcca
      781 gcgtttgcga accgggcgca ccgttcggcg cagcgctggt gctcgactcc gtcatggcgc
      841 cccttccttg acagatgcac tccgcgagag tgatgctctg actatagtgc acatggatgt
      901 gcgataaatg agcagagtgc ttacaaaata cgagaatctg attctcgccg tcaagagagg
      961 gtgacgagtg ggggagtacg agagcatctg gagtgatctt cagggcgtcc cgttctcgca
     1021 gggctacctc gatgcggggg gagtgcgcac ccgctacttg cacgcgggcg atcccggcaa
     1081 gcctgcgctg gtgtttctcc acgggtccgg cggtcacgcc gaggcgtacg tgcgcaatct
     1141 ggaggcccac gccgagcact tctccacgtg gtcgatcgac atgctcggcc acggatacac
     1201 cgacaaaccg ggtcacccgc tcgaggtgcg gcactacgtc gatcacctga tggcgtttct
     1261 gtacgcgatc ggtgccgagc gtgcgcacat cagcggtgaa tcactcggcg gctgggtcgc
     1321 tgcccgcgcc gccgtcgatc actcggatcg aatcgaccgc ctggttctca acaccgctgg
     1381 cggttcgcaa gccgatcccg aggtgatgaa gcggatcatc accctgtcga tggccgccgc
     1441 cgagaacccg gcatgggaga ccgtgcaggc gcgcatcaag tggttgatgg ccgacaagtc
     1501 caaggattac gacgacatcg tcgcgagccg gcagcgcgtc taccgtcagc ctggcttcgt
     1561 cgctgcgatg aaagacatta tggtgctgca ggatccagag atccgggccc gtaacctgct
     1621 cggtcccgcc gagtacgggg cgatcaccgc gccgacgttg gtggtgtgga ccagcgacga
     1681 tccgaccgct gacgtcgccg agggtcgccg catcgcgtcg atgatcccgg gcgcacggtt
     1741 cgaggtaatg ccggggtgtg gtcactggcc gcaatacgag gaccccaaga cgttcaacgc
     1801 ccttcacctc gacttcctgc tgggccgctg acgatgagta accaagcgga cgtcgtcatc
     1861 gtcggggccg gacctgtcgg actcacgttg gccaacatcc ttggcctgca aggcgttcgc
     1921 acgctggtgg tcgatgaacg gccctcagtc atcgactatc cgcgcggcgt cgggctcgac
     1981 gacgaagcga ttcgcacctt ccaggcgatc ggtctcgtcg accggatcct gccgcacacc
     2041 gtgcccaacc agatcctgcg gttcttcgac gccaaacgcc agctgctcgc cgaaatggcg
     2101 ccgcccgacg cgcgtttcgg ctggcccaag cgcaacggct tcgtacagcc catggtcgac
     2161 gccgagttgc tcgccgggct ggaccggttc gaccacgtcg tggtggtgtg gaaccggcag
     2221 atggaaagct gcacggaaac cgacgacggc gtgaccgtcg agttcgcgga tgggcatgag
     2281 gccgtacacg cccgctatgt ggtcggctgc gacggcgggc gcagcgccac ccgccggctg
     2341 atgggcgtgt cgttcgacgg caccacgtca tcgacccgtt ggctcgtcat cgactgcgcc
     2401 aacgacccac ttggccatcc caacagcgag gtgggggcgg atccagcgcg cccatacgtc
     2461 tcgatctcca tcgcccacgg aattcgtcgc ttcgagttca tgattcacgg cgacgagtcc
     2521 gacgaagagg cagaggatcc ggcgttcgtc agacgtatgc tctcgcaact ggtcccgcac
     2581 ccggagcgcg tcgacatcat ccggcaccgc gtctacaccc accactcgcg catcgcaggg
     2641 gcgttccgga agggacggct gatgctggcc ggcgacgccg cacatctgat gccggtctgg
     2701 caggggcagg gctacaacag cggcatccgc gacgcggcca acctcggctg gaagctggcc
     2761 gcggtggtca acggcgacgc cggcgatgcg ctcctcgaca cctacgacgt cgagcgccgc
     2821 aaacacgcca gggcgatgat cgacctgtcc accttggtgg ggcgggtcat ctcgccgacg
     2881 aatcgacggg tcgccgcgct acgggaccgt gtcattcacg ccgcgtccgt cgtgcccacg
     2941 ctcaagcggt acatactcga aatgcggttc aagccaatgc cgcgctacga acagggcgcc
     3001 gtcttccacg ccgagccgcg ctcggaagcc tcaccgacgg gcacgctgtt catccagccc
     3061 cgcatcgaca cccgcgaggc gcagaacgtc ctactcgacg acgttctcgg ccccggcttc
     3121 gccgtgctgt gctggagtaa caacccccgc gcattcctcg gcgacgaagc gttcgaccgg
     3181 tggaaagcgt tgggcgccac gttcgttgcg gcacggccaa tgacccagct gcactggacg
     3241 ggacacgacg accccgacgt cgtcatcgtg ggcgatcgca ccggcgcgct gaaggcgtgg
     3301 ttcgacatcc acaccgattc cgtgttattc ctgcgccccg accgctgcat cgccggcgcc
     3361 tgcatcgccc aacgggcccc agaactttcc gcgtcactgt tcaatgttct tcatctgacc
     3421 cagggaggag gcaacggtgc cactcgccct ctgctgtatg tcgcacagcc cactactgaa
     3481 tcttccggga ccgtcgcagg atctccttga cgacatcgaa tccgcgctcg ccaaggcgcg
     3541 cgacttcgtc accgcgtacg accccgaatt ggtggtcatc ttctcgccgg accattacaa
     3601 cgggttcttc tatcgaacaa tgccgccgtt ctgcatcggc accgcggcgc aaggcgtggg
     3661 cgactacggg acgcacgccg ggccgctcga cgtgccctct gacgtcgcaa ctgattgcgc
     3721 cacagccgtt ctcgaatccg gcatcgacgt ggcgatctcg gccagcatgg acgtcgatca
     3781 cggtacggtg caaccgctgc agaagctgtt cggcgacgcg acaacgcgtc cggtgattcc
     3841 gatcttcatc aactcggtcg ccactccact gggaccgatg cgccgggtgc gggcgctcgg
     3901 cgccgcagtc gggacatacc ttgcgtccct gggtaagcgg gtgctggttg tgggatccgg
     3961 cggcctgtcc catgatccgc cagtgccgac gttggccacc gctccgccgg cggcgcttgg
     4021 ccggatcgtg catggcaacc caatgacacc cgaacagcga caggcccggc aggtagccgt
     4081 catggaggcc gcccgcgaat tcgccggtgg ccagggtccg cttcagccgc tcaatcccga
     4141 ctgggatcac gctttcctcg aactgctcga ctccaaccgg ctctccgagg tcgatgcctg
     4201 gtccaacagc tggatcgagc aggaggccgg ccactccgcg cacgaaatcc gcacctgggt
     4261 tgccgcattc gcagcgatgg ccgcgcacgg acggtaccgg acagagcagc ggttctaccg
     4321 ggctgcgccc gaattgatcg cgggattcgc tatccgaacg gcggtgctcg atgtctgact
     4381 tcgaccacac cgtcgacgtg ctggtggtcg gttccggcgg cggtggcatg accgcggcac
     4441 tggccgccca cacatcgggc ctcgatactc tgatcgtcga gaagtctcct cagttcggcg
     4501 gctccaccgc gctgtcgggc ggcggcatct gggtgcccgg tgccccgagc cagcgcaaaa
     4561 agggctacgt ccccgacccc gacgccgttt tccagtatct gaagcagatc accggcggtc
     4621 tggtcagcga cgcacggctg cgtcagtacg tcgacaccgc gccggtgatg atggagttcc
     4681 tcgaaaacct cagcccgtgg ttcgaattcg tgtggaagcc gggctacgcc gactactacc
     4741 ccgagctgcc aggcggctcc gaactcggca gcaccatcaa cgttccggcg atcgacctgc
     4801 gcaagctcgg tgacgaggag cagaacctgc tgcacccgtt ggcgctggcg cctaagggga
     4861 tctggttcgc ccccaaggac cttcggctgt tctatcaggt gcgtcagaac tggcgcggca
     4921 aagcagttct gctgaaactg atctggcgga tgtttcgcgc gcgggtgttc ggtgaccgga
     4981 tggccgccat cggccagtca ctggccgcgc gcctgcgcct cgccctcaag cagcaggaca
     5041 tcccgctgtg gctgaactcg cccatgaccg aactcatcac cgatatcgac gggaccgtct
     5101 ccggcgcggt cgtcgaacgg gacggcaagc agcagcgcat ccaggcgcgc cgcggcgtga
     5161 ttctggccag cggcggcttc gaccacgaca tgacctggcg tcgtgagcat ctcccggtgc
     5221 tggagaagga ctggagtttc ggcaatcccg ccgcgatggg cgacggtatc cgcgcggggg
     5281 agaaggtcgg cggctcaacc gacctgctcg acgaagcctg gtggttcccg gcgatgtgct
     5341 ggccggacgg ccggctgcag ttcatgctca acgagcggat gatgccgtcg cagttcgtcg
     5401 tcaacggcga cggcaagcgg ttcatcaacg aggctgcgcc ctacatggac ttcgcccacg
     5461 cgatgatcga cggccaccgc tccggtatca cgcacatccc gtgctggctg atcaccgaca
     5521 tccagtcgtt tcaccgatac gtcgtcggcg ggcacctgcc catcccgaag gttccgttcg
     5581 cgcccgtgcc caccggctgg aaggtgccga aggcttggct ggattccggt atcgccaagg
     5641 aggccaacag ctttgaggaa ctcgctgcga agatccaagt gcccgcggcg aacctgcgtg
     5701 cgaccgccga gcgcttcaac gagctggcgc acaggggcca cgacgacgac ttcaaccgcg
     5761 gcgacagcgc ctatgacaac tactacggcg acccgacgct ggccaacccc aacctctatc
     5821 cgctgggcaa gccgccctac tacgcgttcc agatcatcct cggcgacctc ggcacatcgg
     5881 gaggtttgcg cactgatgag ttcgcccgcg tcctgcgtga cgacgacagc gtcgtgaaag
     5941 gcctttacgc cgtgggcaac gcctctgcgg ccgtgatggg acgcagctat gcgggcgcgg
     6001 gcgcgaccat cggcccggcc atgacgttcg gctacgtcgc cgccaaacac attgcacatc
     6061 aacagtccga ctctcgatcc caatcggagg taactacatg aagatctccc tgttctacga
     6121 gttcccgctg ccgcggccgt ggacgcccga tgacgagcac aagctcttcc aggacggcct
     6181 cgatgaagtg gaggcggccg acaaggcggg cttctcgacg gtgtggctga ccgaacacca
     6241 cttcctcgag gaatactgtc actcgacagc accagagatc ttcctcgcgg cagcgagcca
     6301 gcgcaccaag aacattcggt tgggtttcgg catcatgcac ctgccgccgg ccgtcaacca
     6361 tccggcccgg gttgccgagc gcatctcaac cctcgatctc ctctccaacg gccgggtgga
     6421 gttcggcacc ggtgagtcgt cctccgtcgg cgagctcggt ggcttcggca tcgatcccgc
     6481 ggacaagcgc aagatgtggg aagaagccct cgaggtctcg atccgctgca tgatcgagga
     6541 gccgttcacc ggcttcaagg gtgaacatgt cgagatgccg ccgcgcaacg tggtgcccaa
     6601 accgttgcag aaaccgcacc cgccggtgtg ggtggcatgc acccgaccgg cgtcggtatc
     6661 gatggccgcg cagaaaggcc tcggcgcctt gagctttgcc tacacgggac cggggcctct
     6721 gacggagcgc gtcaacgggt actacaagga gttcgaggaa agcgccgtcc cggtcacccc
     6781 ccagatgaac cccaacatcc tcgcgatcgg cggcgatctg tcgatgatgg tcgccccgac
     6841 cgacgagcaa gccatcgaac ggctcgggag gggcggcgga ttcttcgctt tcggcatcat
     6901 gcactactac atgaccggca tgcacacgcc cggccggacc ggcgtctgga agcgacatct
     6961 ggaagagatc gagaaggacc cgaccatcgt ctacgggccc gaccggggcc cgatcggcag
     7021 cccggccacc gtgcgtgagt tcctgcgcgg ctacgaagaa agcggcgtcg acgaactgat
     7081 cctgctgctc actccgcgcc gtcacgaaga gaccatggaa tccattgagc tgatgggcag
     7141 agaggtgctg cccgagttca ttgagcgcca cgagaaggcc gctgccgaga aggcgaagcg
     7201 gatggaaccg atcctcgaga aggccgaggc tcgtcggccg aagtccaatg ctccggaatt
     7261 cgacgagacc tacgcgttcg gcggcctgcc caccggtcgc gagaactaca cggccaacga
     7321 ggtgtcattg gcgatggagg agatgaacgc cggtatcgaa gcggcggcgg cgaaactcaa
     7381 gtcaggggaa gggtggacga gccggaaccc ggacgccgac aagaagtaag ccatgggcca
     7441 gatcgacgaa ttgtggcgct acgacgggag acgcgtcgta gtcaccggtt gtgcctcggg
     7501 tatcggtgag cacgtcgcgc gtcagctcac cgatctcggc gctgaggtcg tcggtctcga
     7561 tgtgcgacgg ccggccttgc agctcaagga gttccacgaa gtcgacttgt ccgacccagc
     7621 gtcgatcgat caggccgcgg caacaatcgg cggtcaagtg gacgccctgt tcaacatcgc
     7681 gggggtgtcc tccgggatcg gcgatccgtt gcgggtggtc acgatcaact tcctcggcac
     7741 ccgccagttc accgaagcat tggtgccgag catgccgccc ggatcggcga tcgcgaacgt
     7801 ctcgtccctt gccgcgtccg gctatcggga gaatgcctcc gtcacagcgg ggctgctgga
     7861 caccgtgacg atggccgagg gaatcgaatg gtgtcagcgc aatcccgcgc cggtcgccga
     7921 cggcgctggc taccggttgt ccaaggaggc gatcatcctc tacgggatgg ccaatgtcgc
     7981 gacgttgggc gccaagggaa tccggatca
//
LOCUS       DASRJA010000787        11087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3510206,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000787 DASRJA010000000
VERSION     DASRJA010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3510206"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /gene="hisG"
                     /locus_tag="VFC01_35420"
     CDS             <1..754
                     /gene="hisG"
                     /locus_tag="VFC01_35420"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZN84947.1"
                     /translation="LTVIDPINNVEFFFLRPKDIAIYVGSGQLDLGITGRDLAQESDA
                     PVRERLALGFGSSSFRYAAPAGREWRVEDLAGKRIATAFPHLVRKDLAGRGIDATVIR
                     LDGAVEISVQLGVADAIADVVGSGRTLGLHDLVAFGEPLCDSEAVLIESATPSDESTA
                     ARDQLAGRVQGVVFGQQYLMLDYDCPRTVLDEATAVTPGLESPTIAPLADPAWVAVRA
                     LVPRRDVNAIMDELASIGAKAILASDIRFCRF"
     gene            823..1305
                     /locus_tag="VFC01_35425"
     CDS             823..1305
                     /locus_tag="VFC01_35425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HZN84948.1"
                     /translation="MTHALVLLLALLIGVIAGLRAMTAPAVIAWGAFLGWIDLDGKWS
                     EWMAHPITVTVLTIFLLVELVTDQLPKTPSRKTPVQFITRLLTGAFAGAVIGSAFFHT
                     FSALGAGMVGAVAGTLGGAEARQRLGAARGGQDRPAAILEDVVAVGGGFLIAFLVSLV
                     "
     gene            1316..2686
                     /locus_tag="VFC01_35430"
     CDS             1316..2686
                     /locus_tag="VFC01_35430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HZN84949.1"
                     /translation="MTEKFDAIIVGAGQAGPPLAGRLTDAGQTVAVIERKLVGGTCVN
                     TGCIPTKTLVASAHAAHLARRGTEYGVGTGDISVDMAKVKARKDKIMLDDRHGVESWL
                     TGMKGCTLIRGHARFEGPHTISVDGRVLEADKIFLNVGGRAVAPDMPGLSDIDYITNV
                     GILELDTLPEHLVIIGGSYIALEFAQMYRRFGARVTVIEKGPRLTSREDEDVSATIKE
                     ILEAEGIDVVVGATAIQFARHDSGFQVVPREGAEPIAGTHLLMAVGRQPNTEDLGLDK
                     AGVQTDERGYIVVDDQLRTNVEHIWAMGDCNGRGAFTHTSYNDFEIVAANLLDDDPRR
                     VSDRVPTYALYIDPPLGRAGLTVDQVRKSGRKALVGKRPMTRVGRAVEKGETQGFMKV
                     VVDAETEEILGAAILGVGGDEVIHAILDIMTAKKPYTAISRTMHIHPTVSELVPTLLQ
                     EMKPLQ"
     gene            complement(2637..3284)
                     /locus_tag="VFC01_35435"
     CDS             complement(2637..3284)
                     /locus_tag="VFC01_35435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZN84950.1"
                     /translation="MHHNGSDIAAPTVVIAYHSGFGHTAVLADAVAVGVREAGADVSV
                     IAVDRITDTEWDVLDAADGIIFGSATYMGNVSAGFQTFAEQTGRRCLNGTWRDKVAAG
                     FTNSGAKSGDKVNTLVSLAIFAAQHHMHWVSLGLGAGWNSALGSENDLNRLGFWLGAG
                     AQTDVDANHDQVHPSDVQTCRHLGHRVAMVTRQLNIGRSATAAASSPAAASGPAR"
     gene            complement(3293..3784)
                     /locus_tag="VFC01_35440"
     CDS             complement(3293..3784)
                     /locus_tag="VFC01_35440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZN84951.1"
                     /translation="MTGREGRLTIEGEKAVLTFERRLPYPIEAVWSAITDPAHRNTWM
                     GETTIDAREGGVIEMVPTGPPQPPEQKKMTGRIRVWDPPHVFEHEWNQRIVEESVVRY
                     ELSADGDGTLLRFSHRGLGVRNAQGFLPGTHAFLDRLEAYLGGEALPNWAQRYREVAE
                     SNR"
     gene            complement(3781..4098)
                     /locus_tag="VFC01_35445"
     CDS             complement(3781..4098)
                     /locus_tag="VFC01_35445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZN84952.1"
                     /translation="MDVFEAVAEPSRRALLDALIDGERTAGELVATLPGLTQPTVSRH
                     LRVLREVGLVEVRPDAQRRIYALRADGLVAIDDWINRYRRYWADHLDALERHLEKTHK
                     EPK"
     gene            complement(4159..4956)
                     /locus_tag="VFC01_35450"
     CDS             complement(4159..4956)
                     /locus_tag="VFC01_35450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZN84953.1"
                     /translation="MIDCHYRRLAADGEFQVFESTDGTRSNWDPEIQHGSPPLALMTK
                     LIEELAGDSGLRVGRLTLDILGAIPVTRIRARAWVERPGSRISIMAAEILAPRPDGSE
                     RAVARVTAWLLATSDTADATTDRYPPLVEGDSVPVPHSWDGATGYLETVRWRRQRVAD
                     GQTPVAWLSPLVPLVDSEPTTDLQRLAMVADSVNGVGAALDPEKFVFMNTDTVVHLHR
                     LPAGNDFGLRARGSIGPDGIGVTTADIFDRQGFIGTVAQTLFVQRRP"
     gene            complement(4953..5831)
                     /locus_tag="VFC01_35455"
     CDS             complement(4953..5831)
                     /locus_tag="VFC01_35455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecB family exonuclease"
                     /protein_id="HZN84954.1"
                     /translation="MSDEPVPEVRRPALSPSRASDFKQCPLLYRFRAIDRLPEPPSTA
                     QVRGSVVHAALEQLFALPAAERGRDTAISLVGPAWDRVVAERPELAAEIEPALRADLL
                     DEARALLSGYYRLEDPTRFDPQSCEQRVEVELSDGTLLRGFVDRIDVAPTGELRVVDY
                     KTGNAPPEARALAEFKAMFQMKFYAVALLRSRGVLPARLRLLYLSDGQVLDYTPDLDE
                     LVRFEKTLMAIWRAIQSAGATGDFRPHPSRMCDWCAHHSHCPVFGGTPPPYPGWPKAP
                     DEGDDDTVLHTSEPAA"
     gene            complement(5939..7393)
                     /gene="tnpB"
                     /locus_tag="VFC01_35460"
     CDS             complement(5939..7393)
                     /gene="tnpB"
                     /locus_tag="VFC01_35460"
                     /inference="COORDINATES: protein motif:HMM:NF038280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family element RNA-guided endonuclease
                     TnpB"
                     /protein_id="HZN84955.1"
                     /translation="MASFEIPQGWKIQAYRFALDPTPAQEKTLLSHAGGARFAYNTML
                     AAVRANLDQRHAEKSYGIADSDLIPCMSWSFQSLRNDWNRRKHSVAVDADGTPWWPQN
                     SKEVYANACRALAEALANWGASKKGTRRGDRMGFPRFKTKNGAPKAFSFSTGVIRLGP
                     DRHHITLPRLGTIRTHEATRKLARRVEAGNARIIKATVRFERGRWLVSFTCIVARDIG
                     RPAHVKRGAKVVGIDAGVKDLIVVADADGNELARHRAPRELKAAQRTLRALHRKAARQ
                     VGPWDPTTGTRREPSQGWAATQRDIRRAHARVANLRTDRTHKLTTQLSQTHEVVGVET
                     LAVKNMMAAGGTHKRGLNRALADAGLGELLTQLDYKSGWYGSRVVKAERWYPSSKLCS
                     RCGVVKSKLHLSERTYQCDSCGLVINRDLNAAVNLAHYAHNETDPSTGVVTGGADRKP
                     SLAGDAGGSETRTRAEVGANADDGGSASPKGEAA"
     gene            7617..8456
                     /locus_tag="VFC01_35465"
     CDS             7617..8456
                     /locus_tag="VFC01_35465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HZN84956.1"
                     /translation="MPRTGPFEVGDRVQLTDAKGRHYTMLLTPGGEFHTHRGIISLDT
                     VIGLPEGSVVKSTSGDQFLVLRPLLVDYVISMPRGAQVVYPKDAAQIVHEGDIFPGAR
                     VLEAGAGSGALTCSLLRAVGPDGQVTSYEVRDDHAEHAARNVATFFGERPANWNLVTA
                     DLVDYAGPEVDRVVLDMLAPWEVLDAVANALVAGGVLMIYVATVTQLSRTVEALRVQQ
                     CWTEPRAWESMQRGWHVVGLAVRPQHTMRGHTAFLISVRKLAPGAVAPMPLRRKRQLG
                     GSV"
     gene            complement(8453..8749)
                     /locus_tag="VFC01_35470"
     CDS             complement(8453..8749)
                     /locus_tag="VFC01_35470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 family protein"
                     /protein_id="HZN84957.1"
                     /translation="MWIGWLEFDLLLGDVRSLKQKRSVIRPVIAELQRRFAVSAAESG
                     AQDLHRRANVGIAVVSADRGHVVDVLDAAERLVAGRPEMELLSARRGLRRSSDD"
     gene            complement(8760..9119)
                     /locus_tag="VFC01_35475"
     CDS             complement(8760..9119)
                     /locus_tag="VFC01_35475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84958.1"
                     /translation="MLTDLMGRLADPDIPSPDKVGLVQYGTPADAVAADRFTKALHDS
                     GYAPLTFEATDLAWAQDPPGNVIADITIKAGGAQAGKDLNFPMEFSPQQDGWQLTRKT
                     ADLLLQMGPLPTATPTP"
     gene            complement(9305..9802)
                     /locus_tag="VFC01_35480"
     CDS             complement(9305..9802)
                     /locus_tag="VFC01_35480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84959.1"
                     /translation="MTLKTLVTGVAAAALVGGAAAGVTSIASPTVTSAPAVQPVVWDI
                     PMPQAPAPDLEAPLLQTLQALASPGSFAGKRAFIQGGIGRIEGIAADRAYNNAAAEGK
                     FPLSFYIDKNMIDQQGGVVTVDVTATSALGTSATQNVQFVAGPSPTGWQISKASALNL
                     LSAAG"
     gene            9947..>11087
                     /locus_tag="VFC01_35485"
     CDS             9947..>11087
                     /locus_tag="VFC01_35485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN84960.1"
                     /translation="MNESERSEAYGASHESGMSSDDAAELEELRREAALLREQLENAV
                     GPQSGLRSARDVHQLEARIDSLAARNAKLMETLKEARQQLLALREEVDRLGQPPSGYG
                     VLLGVQDDDTVDVFTSGRKMRLTCSPNIDTKTLKQGQTVRLNEALTVVEAGNFEAVGE
                     ISTLREILSDGHRALVVGHADEERIVWLAEPLVAAEHLPDGYDDERDDDLRPRKLRPG
                     DSLLVDTKAGYAFERIPKAEVEDLVLEEVPDVSYNDIGGLGRQIEQIRDAVELPFLHK
                     ELYREYSLRPPKGVLLYGPPGCGKTLIAKAVANSLAKKMAEVRGDDAREAKSYFLNIK
                     GPELLNKFVGETERHIRLIFQRAREKASEGTPVIVFFDEMDSIFRT"
BASE COUNT         1869 a         3596 c         3651 g         1971 t
ORIGIN      
        1 cctgaccgtc atcgacccga tcaacaatgt cgagttcttc tttctgcggc ccaaagacat
       61 tgcgatctac gtcggatcgg gacagctcga cttgggcatc accggtcggg atctggctca
      121 ggaatcggat gcaccggtcc gtgagcggct cgcgctcggc tttgggtcat cgtccttccg
      181 ctacgcggcg cctgcgggcc gggaatggcg tgtcgaggac ctcgcgggca agcggatcgc
      241 caccgcgttt ccccacctgg tacgaaaaga cttggcggga cgtgggattg acgccaccgt
      301 gatccggctc gatggcgcgg tggagatttc ggtgcagctc ggggtcgccg acgcgatcgc
      361 cgatgtggtg ggctcgggcc gaactctcgg gctgcatgac ctggtcgcat tcggtgaacc
      421 gctgtgcgat tcggaggcgg tgctgatcga gagtgcgacg ccgagcgatg agagcaccgc
      481 cgcccgagac cagctggcgg gccgggtgca gggcgtggtg ttcggacagc agtacctgat
      541 gctcgactat gactgtccgc gcaccgtcct cgacgaagcc accgcggtca ctccgggact
      601 cgaatcgccc accatcgctc ctctcgccga tccagcgtgg gtcgcggtgc gggcgctggt
      661 tccacgccga gacgtcaacg cgatcatgga cgaacttgca tcgatcggcg ccaaggcgat
      721 tctggcctct gacatccggt tctgccgctt ctgattcaac cggaatattg acgcgctgcg
      781 tgttagcgtc cggttgtcct ttttgccggt cgggaggttc ggatgacgca cgcactggtt
      841 ctgctgctcg ctctgctgat cggtgtgatc gctgggctgc gggcaatgac agcgcctgca
      901 gtaatcgcgt ggggtgcgtt cctcggctgg atcgacctcg acggcaagtg gtcggagtgg
      961 atggcacatc cgatcaccgt cacggtgctg accatcttcc tgctggtgga attggtcacc
     1021 gaccaattgc cgaagacgcc cagccgcaag acaccggtcc agttcatcac tcggcttctc
     1081 accggcgcct tcgccggagc ggttattggt agcgccttct tccatacctt cagcgcgctc
     1141 ggcgccggca tggtgggtgc cgtggccggc acacttggtg gtgccgaggc ccgtcagcgg
     1201 ctgggcgcgg cgcgcggtgg tcaggatcgg cccgccgcga tcctcgagga cgtcgtcgct
     1261 gtcggcggcg gattcctgat cgcgttcctc gtcagcctcg tctgagtcac cttccatgac
     1321 agagaaattc gacgcgatca tcgtcggcgc gggtcaggcc ggcccgccgc tcgctggcag
     1381 actgaccgat gccggacaga ccgttgcggt gatcgaacgc aaactggtcg gcggcacgtg
     1441 tgtgaacacc ggctgcatcc ccaccaagac gctggtcgcc agcgcccatg cggcccattt
     1501 ggcccgtcga ggcactgaat acggcgtcgg caccggcgat atcagcgttg acatggcaaa
     1561 ggtcaaggcg cgcaaggaca aaatcatgct cgacgaccgg catggggtcg aatcgtggtt
     1621 gacgggcatg aagggatgca cactcattcg tggccatgcc cgtttcgaag gcccgcacac
     1681 catcagtgtc gacggccggg tactggaagc cgacaagatc ttcctcaacg tcggtggccg
     1741 cgcggtggca cccgacatgc ctgggctctc ggacatcgac tacatcacga acgtcggcat
     1801 cctcgaactc gacaccctcc cagagcacct ggtgatcatc ggcggcagtt acatcgcgct
     1861 ggagttcgca cagatgtacc gccggttcgg cgcccgggtg accgtgatcg agaaggggcc
     1921 gcggctgaca tcccgtgagg acgaggacgt ctcggcgacc atcaaagaga tcctcgaagc
     1981 cgaaggcatc gatgtcgtgg tcggcgccac cgccattcaa ttcgccaggc acgacagcgg
     2041 ttttcaggtt gtacctcgcg aaggcgccga accgatcgcc ggaacacacc tattgatggc
     2101 ggtcggtcgc cagcccaaca ccgaggacct gggattggac aaggcgggag tgcagaccga
     2161 cgaacgcgga tacatcgtcg tcgacgacca gctgcggacc aacgtcgagc acatctgggc
     2221 gatgggcgac tgcaatggcc gcggcgcgtt cacgcacacc tcgtacaacg acttcgagat
     2281 cgtggcggcc aacctgctgg acgacgaccc acggcgggtc agcgaccgcg tcccaaccta
     2341 cgcgctctac atcgatccgc ccttgggccg cgccggcctg accgtcgatc aggtgcgcaa
     2401 gtccggccga aaagcgttgg tgggcaagcg gcccatgacg cgcgtcgggc gcgccgtcga
     2461 gaagggggag acccagggct tcatgaaggt ggtcgtcgac gccgaaaccg aggagatcct
     2521 cggtgcggcc attcttgggg tcggcggtga cgaagtcatc cacgcgatcc tcgacatcat
     2581 gacggccaag aagccctaca ccgcgatctc gcgcaccatg cacatccacc cgacggtcag
     2641 cgagctggtc ccgacgctgc tgcaggagat gaagccgctg cagtagcaga ccggccgatg
     2701 ttgagttgtc gggtcaccat ggccacacgg tggccgaggt gtcggcacgt ctgcacatcg
     2761 gacgggtgga cctgatcgtg gttggcgtca acgtcggtct gtgcacccgc gccaagccag
     2821 aacccgaggc ggttgaggtc gttctcgctg ccgagcgcgc tgttccaccc ggcgcccaaa
     2881 cccaggctga cccaatgcat gtggtgttgg gccgcgaaga tcgccagtga caccagggtg
     2941 ttgaccttgt cgccgctctt ggcccccgag ttggtgaaac ccgcggcgac cttgtcgcgc
     3001 cacgttccgt tgaggcagcg gcggcccgtc tgttcggcga acgtctgaaa ccccgccgac
     3061 acgttgccca tatacgtggc actgccgaaa atgatgccgt ccgcggcatc gagcacgtcc
     3121 cactcggtat cggtgatgcg gtcgacggcg atcacgctga cgtcggcgcc agcttctcga
     3181 acaccgacgg cgacggcgtc ggcaagcacg gcagtgtgcc cgaacccaga gtggtatgcg
     3241 atcaccacgg tgggtgcagc gatgtcagaa ccattgtggt gcatggaaat tcctatctat
     3301 tcgattccgc gacttcgcga tagcgctggg cccagttcgg cagcgcctcg ccgccgaggt
     3361 atgcctccag ccggtccagg aaggcgtgcg ttccaggcag gaacccttgt gcgttgcgca
     3421 cgccgagccc gcggtggctg aatcgcagca gcgttccatc accgtcggct gacagctcat
     3481 agcggaccac gctctcctcg acgatgcgct ggttccattc gtgctcgaac acatgcggcg
     3541 gatcccatac ccggatgcgt ccggtcatct tcttctgctc cggcggttgc ggcggacccg
     3601 tcggcaccat ttcgataacg ccgccttcgc gggcgtcgat tgtcgtctcg cccatccatg
     3661 tgttgcggtg cgccggatcg gtgatcgcgg accacaccgc ctcgatgggg tagggcaggc
     3721 ggcgctcaaa tgtgagcaca gccttctcgc cctcgatggt gagtcgtcct tcgcgcccgg
     3781 tcatttcggc tccttgtgcg tcttctccag gtgccgctcg agggcgtcga ggtgatcggc
     3841 ccagtagcgg cggtaacggt tgatccagtc gtcgatcgcg acgaggccgt cggcgcgtag
     3901 tgcgtagatc cgccgctgcg cgtcgggccg gacttcgacg aggccgactt cacgcagcac
     3961 ccgtaggtgc cgggacaccg tgggctgggt caggccgggc agggtcgcga cgagctcgcc
     4021 agcagtccgc tcaccgtcga tcagcgcatc cagcagcgcg cgccgactcg gctcggcgac
     4081 tgcttcgaag acgtccatgg agcgagtatt gcacgacatc tatatagatg caagcgcatg
     4141 cagcaaaccg gctgtcgatc aggggcggcg ctgcacaaac agcgtctggg caacggtgcc
     4201 gataaaccct tggcggtcaa agatatccgc ggttgtcaca ccgatgccgt ccggtccgat
     4261 cgacccgcgt gcgcgaaggc cgaaatcgtt gcctgcgggt aaccggtgca ggtgcacgac
     4321 ggtgtcggtg ttcatgaaga cgaacttctc cgggtccagc gcagcgccga cgccgttgac
     4381 ggaatcggcg accatggcga gccgttgtag gtccgtcgtc ggctcagagt cgactagcgg
     4441 aaccagcggg ctcaaccagg ccaccggagt ttgcccgtcg gcgacgcgct gcctgcgcca
     4501 acgcacggtc tcgagatagc ccgtggcgcc gtcccagctg tgcggcaccg ggaccgaatc
     4561 gccttcgacg agcgggggat agcggtcggt agtggcgtcg gcggtgtcgc tggtcgcgag
     4621 cagccatgcg gtcacacggg ccactgcgcg ctcggacccg tcgggccttg gcgccaatat
     4681 ctcagcggcc atgatcgaga tgcgggagcc gggacgctcg acccaggccc gcgcccggat
     4741 ccgcgtgacg ggtatcgcgc cgagaatgtc gagggtgagt cggccgacgc gcaatccgga
     4801 atcgcccgcc agctcttcga tcagtttggt catcagcgcg agcggcggtg agccgtgctg
     4861 gatttcggga tcccaattac tccgcgtccc gtcggtggac tcgaacactt gaaattcgcc
     4921 gtcggcggca agccgacggt agtggcagtc gatcacgcag caggctccga ggtgtgcaac
     4981 accgtgtcat cgtcgccctc gtcgggagcc ttcggccagc cggggtaggg cgggggagtg
     5041 ccaccgaaca ccgggcagtg ggagtggtgc gcgcaccagt cgcacatccg cgacggatgc
     5101 ggacggaaat cgcctgtggc accggcggat tggatggccc gccaaatcgc catcaatgtt
     5161 ttctcgaagc gcaccagctc gtcgagatcg ggggtgtagt cgagcacttg accgtcggac
     5221 aggtagagca gccgcagccg ggcgggcagc acaccacgcg accgcagcag tgcgaccgca
     5281 tagaacttca tctggaacat cgccttgaat tcagcgagcg cccgggcctc gggcggcgca
     5341 ttgccggtct tgtagtcgac tacccgcagc tcgccggtcg gtgccacgtc gatgcggtcg
     5401 acgaagccgc gcagcagggt gccgtcggag agttcgacct caacccgctg ttcgcagctc
     5461 tgtgggtcga accgcgtcgg gtcttcgagc cggtagtagc ccgacagcag cgcgcgcgcc
     5521 tcgtcgagca ggtcggcccg cagcgccggc tcgatctcgg ccgccagttc cggtcgctcc
     5581 gcgaccaccc gatcccatgc cgggccgacg agcgagatcg cggtgtcgcg gccccgctcg
     5641 gcggcgggca acgcgaacag ctgctcgagc gcggcgtgca ccaccgaccc gcgtacctgc
     5701 gcggtggagg gcggctcggg cagccggtcg atcgcgcgga accggtacag cagggggcac
     5761 tgcttgaagt cgctggcccg cgaaggagac agcgccgggc gccgaacctc aggcaccggc
     5821 tcgtcactca tgcgcgattt gccttcggct tctggctcgc cgctcataaa ggccaccgta
     5881 gtatgaccat gctccgacaa ccgttacctg aaatgtgtgt gtttgtgtag ttgggcattc
     5941 aggcagcctc ccctttggga gaggcactcc caccgtcatc ggcgttggcg cctacttcgg
     6001 ctcgggttcg ggtttcgctg ccaccagcgt caccagcgag tgacggctta cggtcggctc
     6061 cacccgtgac gaccccggta ctcgggtccg tttcgttgtg cgcgtagtgc gccaggttga
     6121 ctgctgcgtt gaggtctctg ttgatgacaa gcccgcagct gtcgcactgg taagttcgct
     6181 cggacaggtg cagtttggat ttcacgacac cacagcgaga gcacaatttc gagctgggat
     6241 accagcgctc ggccttgacg acgcgtgagc cgtaccatcc gctcttgtag tcgagttggg
     6301 ttagcagttc accgaggccg gcatccgcca gtgcgcggtt aagtcccctc ttgtgtgtcc
     6361 cgcctgcagc catcatgttc ttcaccgcca aggtctcaac cccgacgacc tcgtgggtct
     6421 ggctcagctg ggtggtgagt ttgtgggtgc ggtctgtccg caggttggcc actcgcgcgt
     6481 gtgcgcgtcg gatatcgcgt tgtgtggccg cccagccttg cgagggctca cgcctggttc
     6541 cggtggtggg gtcccagggg ccgacctggc gtgccgcttt gcgatgcaac gcgcgcagcg
     6601 tgcgctgcgc tgccttgagt tcacggggtg ccctgtggcg tgccaattcg ttaccgtcgg
     6661 catcagcaac cacgatcaag tctttgacac cggcatcaat gccgacgacc ttggcgcctc
     6721 gtttcacatg tgccggacgg ccgatatctc gggccacgat gcaagtgaac gacacgagcc
     6781 agcgacccct ctcgaaccgc acggtcgcct tgatgatccg tgcattgccg gcttcgacac
     6841 gtcgagcaag ctttcgtgtg gcctcgtggg tcctgattgt gcccagtcgg ggcaaagtga
     6901 tgtggtgtcg gtccggtcca agccgaatca cgccggtgga aaatgagaac gccttaggcg
     6961 caccgttttt cgtcttgaac cgaggaaacc ccatacggtc cccgcggcga gttcccttct
     7021 tcgacgcccc ccagttcgcc aatgcctcgg ccagcgcccg acacgcgttg gcgtaaacct
     7081 ctttcgaatt ctgaggccac cacggcgtcc cgtctgcgtc gactgcaacc gagtgtttgc
     7141 gtcggttcca gtcattacgc agcgactgaa acgaccaact catacacggg atcagatccg
     7201 aatccgctat cccgtacgac ttctcggcgt ggcgttgatc gagattggcc ctcaccgccg
     7261 ccaacatcgt gttataggcg aaccgggcac cgccggcatg tgacaagagt gtcttctcct
     7321 gtgccggggt cgggtccagc gcgaaccgat acgcttgaat cttccagccc tgcggaatct
     7381 caaaactcgc catgccctct caccccgcct cgagcgttcg ttcgcctgcg accacagtgc
     7441 ggctccacaa cccacggttg cagacaggtc cgacaaaaaa cctgtgcctt cgagcgccgc
     7501 cccagcctcc ccgcgactat gcggtggcgt tgggtcgcgt ctcggttggc cccacaaacc
     7561 cactccgaca accccgagca cccgtgtcgg cgcgtctgct ggcaggctga tcagctgtgc
     7621 caaggacggg tccgttcgaa gtcggtgatc gcgtgcagct caccgacgcc aagggcaggc
     7681 actacacgat gctgttgacg cccggcggcg agttccacac ccatcgcggg atcatctccc
     7741 tcgacaccgt catcgggctg ccggaaggca gtgtcgtcaa gtccacgagc ggcgaccagt
     7801 tcctggtgct gcggccgctg ctcgtcgatt acgtgatatc gatgccgcgc ggagcgcagg
     7861 tcgtgtaccc gaaggacgcc gcccagatcg tgcacgaggg cgacatcttt ccgggcgcgc
     7921 gcgtactgga ggccggtgcc ggttccggtg cgctgacgtg ctcgctgctg cgcgccgtcg
     7981 gtcccgacgg tcaggtgacg tcgtacgagg tgcgcgacga ccacgccgag cacgccgccc
     8041 gcaatgtcgc gacgttcttc ggtgagcggc cggccaattg gaacctcgtc accgccgacc
     8101 tcgtcgacta cgccggtccg gaagtggacc gcgtggtgct cgacatgctc gcgccgtggg
     8161 aggtcctcga tgccgtcgcc aatgcccttg tcgcgggcgg ggtcctgatg atctacgtcg
     8221 ccaccgtcac gcagctgtcg cgcaccgtgg aagcgctacg cgtgcagcag tgctggaccg
     8281 agccgcgggc gtgggagagc atgcagcgcg gttggcatgt cgtcggcctc gcggtgcgcc
     8341 cccagcacac gatgcggggg cacacggcct ttctgatcag cgtgcgcaaa ttggcgccgg
     8401 gggccgtcgc gccgatgccg ctgaggcgca aacgccagct gggcggcagc gtctagtcgt
     8461 cgctactacg ccgcagtccg cggcgggccg acaacaattc catctccggc cgtcccgcca
     8521 ccaaccgctc ggcggcgtcg agcacgtcga cgacatgtcc tcgatcggcc gataccaccg
     8581 cgatgccgac gttcgcacgg cggtgcaggt cctgggcgcc cgactcggcg gccgacaccg
     8641 caaacctgcg ctgcaattcg gcgatcaccg ggcggatgac agagcgcttc tgcttgagcg
     8701 aacgcacatc accgaggagc aggtcgaact ccagccagcc gatccacacg ccggcgtgct
     8761 cagggagtgg gcgtagcggt cggcagcgga cccatctgga gcagcaagtc cgcggtcttg
     8821 cgggtcagct gccacccgtc ctgctgcggg ctgaattcca ttgggaagtt caaatccttg
     8881 ccggcctgtg ctccgccggc cttgatggtg atgtcggcga tgacgttacc cggcgggtcc
     8941 tgcgcccagg ccagatcggt ggcctcgaac gtcaacggcg cgtagccgct gtcgtgcagc
     9001 gccttggtga acctgtccgc tgctactgcg tcggcaggcg tgccgtactg gaccaggccg
     9061 accttgtcgg gactggggat gtcggggtcg gccagtcggc ccatcaagtc cgtcagcatg
     9121 gtcgctggcg gtaacggcgc cccggtttgc gtcggtgacg gcggaaccgt cggcggtcga
     9181 ctgggcggga aggccgccgg gcttgatgcg gttcctgtgt catcgctaca gccgctaagc
     9241 ccgagcgccg ccactaatgt gacggcgctc aggactgcgg caatgctgcg atgcactcgg
     9301 tcggtcagcc ggccgccgag agcaggttca gggcggaggc cttcgagatc tgccagccgg
     9361 tcgggctcgg cccggccacg aactggacgt tctgcgtggc gctggtacca agggcagacg
     9421 ttgcggtgac gtcaaccgta accacgccac cctgctggtc gatcatgttc ttgtcgatgt
     9481 agaagctcag tgggaatttg ccttccgcgg ctgcgttgtt gtacgccctg tcggcggcga
     9541 taccttcgat cctgccgatg ccgccctgaa tgaaggcacg cttgccggcg aacgagccgg
     9601 ggctggccag cgcctgcagt gtctgcaaaa gcggagcctc caggtccggc gccggtgcct
     9661 gcggcatcgg gatgtcccac acgaccggtt gcacggccgg agccgacgtc acagtggggg
     9721 atgcaatgga agtcacacct gctgccgcac cgcctaccag tgcagccgct gctacgccgg
     9781 taacgagggt tttcagggtc acgattgtcc tttcgtcggc ccaactcacc agagaggtta
     9841 acagtgttgc tggtgtgtcg aattcctaca ccgcacacaa aggctgaatc gccggtagcg
     9901 ttgaagttgt tactgcacca actcccggtg cgggaagggg cgcaacatga atgagtcaga
     9961 gcgttctgaa gcgtacgggg catcccacga atcagggatg tccagcgatg atgccgcgga
    10021 gttggaagag ctgcgtcgag aggccgcgct tctgcgcgag cagctggaga acgccgtagg
    10081 tccgcagagt ggacttcgca gcgcccgcga tgtccaccag ctcgaggcgc ggatcgactc
    10141 gctcgcggcc cgtaacgcga aactaatgga aactcttaaa gaggcccgtc aacagttgct
    10201 ggcgctgcgc gaggaggttg accggctggg tcagccgcct agcggctatg gcgtattgct
    10261 gggtgtgcaa gacgacgaca ccgtcgacgt gttcacctcc ggccgcaaga tgcgcctgac
    10321 gtgctcgccg aacatcgaca ccaagacgct caagcagggc cagaccgtgc gcctgaacga
    10381 ggcgctcacc gtcgtcgagg ccggcaactt cgaagcggtc ggtgagatca gcacactgcg
    10441 cgagatcctg tccgacgggc accgcgcgct cgtggtcggc cacgccgacg aggagcgcat
    10501 cgtctggctg gccgagccgt tggtggccgc cgagcacctg cccgacggct acgacgacga
    10561 acgtgacgat gatctgcggc cgcgcaaact gcggcccggt gattcgctgc ttgtcgacac
    10621 caaggccggg tacgcgttcg agcgcatccc gaaggccgag gtcgaggatc tggtcctcga
    10681 agaggtgccc gacgtcagct acaacgacat cggcggcctt ggccggcaga tcgagcagat
    10741 ccgcgacgct gtggagctgc cgttcctgca caaggagctg taccgcgagt actcgctgcg
    10801 tccacccaag ggtgtgctgc tctacggccc gcccggctgc ggcaagacac tgatcgccaa
    10861 ggccgtggcc aactcgctgg ccaagaagat ggccgaggtg cgtggcgacg acgctcgtga
    10921 ggcgaagtcg tacttcctca acatcaaggg ccccgagctg ctcaacaagt tcgtcggcga
    10981 gaccgagcgg cacatccggc tgatcttcca gcgggcgcgc gagaaggcgt ccgagggcac
    11041 gccggtcatc gtgttcttcg acgagatgga ctccatcttc cgcaccc
//
LOCUS       DASRJA010000788         7678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2909400,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000788 DASRJA010000000
VERSION     DASRJA010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7678
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2909400"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VFC01_35490"
     CDS             <1..629
                     /locus_tag="VFC01_35490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014383281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZN84961.1"
                     /translation="LAVVPYFSTVIGTVGMDLDQLRAWFDRPAATTLQIAAAPGVDPK
                     QLIQDVRNVVPAPNYVYDGSTALAGMEAPLRQSMFIANAVWIIVVLVAAVALLNTLTL
                     SVLERRREIGVLRAMGSSRRFTLGMVLTEAVGIGVVGGTLGLLFGLTDQWLYSLVSGD
                     IMNFNVDFQPSPMALVFTVGAIAVSLLGSLPPARKAARLNIIEAVSVE"
     gene            complement(643..1728)
                     /locus_tag="VFC01_35495"
     CDS             complement(643..1728)
                     /locus_tag="VFC01_35495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84962.1"
                     /translation="MSEGTTMQNAKHIGGIWALAVALGMGMAVANTPAVALATPDDTG
                     KGSSSDGSSPSSSSPGTESHHPRRVDPPGAVKRSESQNISTTTDSSSSTEAATTPATS
                     DAPGTDARRSRRGTPREESGDASVKVSPKPLAVNAPTTDATPRRRDAIRVVHTEQPSF
                     ASTTPSAAAAPVAAITAEPTTSPIAPAEKATTPTATTPRPADPLSRLVSMVINAVLSP
                     FANTAPTAPVQSPAEWTLLAFARREFEQASTPSTVANTSGVTTSAVDPGFISSTHNFG
                     LFSITSAADPDDNHYVAFVLQTPFFTNVLTSGSDPEDNLGFGAASIGLPGHTVNTFIS
                     PFLTFSIAIPVTDPFAELFTELVRLGF"
     gene            complement(1782..2432)
                     /locus_tag="VFC01_35500"
     CDS             complement(1782..2432)
                     /locus_tag="VFC01_35500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN84963.1"
                     /translation="MTRRNPLFPYVYRLGMPVFDKLFYNRYRRDALSEATGRLLMIGV
                     GPGTDLAFLPPAVTAVAAVEPVASFRRMASAVAERCGIAVDIVDGVAEAIPFPDNSFD
                     SVHVALVLCSVDDVAATLGEIRRVLAPGGRLVVLEHVRGDGAMGRFQDLIAKPWSWLA
                     SGCHPNRRTVDAIAAAGFDTGGLRSIRRTLVPPPCTPHLRGFATVITTDDGVSGEI"
     gene            complement(2429..2998)
                     /locus_tag="VFC01_35505"
     CDS             complement(2429..2998)
                     /locus_tag="VFC01_35505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HZN84964.1"
                     /translation="MRGAIDAPSRWLWLVKWCVLAVPHYPILIGLYLVYPFSTLVAGV
                     AILFTGRYPRPIFDFNVGVLRWSWRVMNYRFPMNTTDKYPPFTMAPRPDYPGDLEVDY
                     PEQLTNWAVLVKWWLLAFPQIILCWAMEPFLQILCVINAGWLLFAGTNHQGMFDLLMG
                     IVRWRYRVAVYVSLMSDEYPPYRMDLGDR"
     gene            complement(3016..4221)
                     /locus_tag="VFC01_35510"
     CDS             complement(3016..4221)
                     /locus_tag="VFC01_35510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN84965.1"
                     /translation="MGRNGPAVPPTRPLRILVVGAGVGGISVARGLLRDGHHVTVFER
                     RPDARAGGGAVTIWSNGSTVLQQLGVDMDGAGQLLSSVRVLTSTGRPLVTLDLTTIED
                     RLGAPIQMVPRRVVLDRLLDGFPCDRIRYNRRVVDVVSADGGVRVEFEDGSAAEGDLL
                     IGADGLHSKVRDIVGAREAEPTGWCSWQGLATLPTVPDKRVALLVIGERGNLGLWPAG
                     GSEVQWWFDLPWSYRFVRPERPIDVIREHFAGWCDAVDGLLATLTDDDLADSPYPHFR
                     HPIPPPGRGRLTLLGDAAHTMPPTLAQGTNQALLDTMVLCKALSDHQGGDLPKVLRWY
                     EKTRRRSVAAVSRVTSLQVSHGEAVLRPAAVIPDRYMTWALTTFLSSVSHRRISADVS
                     RDLAATTSS"
     gene            4359..5846
                     /locus_tag="VFC01_35515"
     CDS             4359..5846
                     /locus_tag="VFC01_35515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84966.1"
                     /translation="MLDPVVVGVFAAAVSLVGAGRPSFWYDEAATISASYSRSLGQLW
                     QMLNNVDAVHGLYYLLMQGWFHVVPPTEFWSRAPSGLAVGAAAAGVVVLAKQFSTRTV
                     AVSSGLVCGILPRATWAGIEARPYAISMAAAVWLTVLLVHATRRNTAWLWLSYGIALA
                     TSILLDVYLALMLLAHFAFVCLHRRARTVLVRFAITSVLTGAAVTPFALEVVGQAHQI
                     VWIAPVGRRTIEDVGVQQYFERSPPFLILSALIVATAIALWLFTSARLTQPDRQLLTL
                     AIGWMAIPTAMILIWSAVVQPIYTPRYLCFTAPAIALVLGVCIGALAVRPWAAAAVVT
                     ALALAAGPNYLLVQRGPYAKYGMDYSQVADLITAKAAPGDCLLVNDTVSFMPAPMRPL
                     LAARPDAYHKLADLSVWQRATDRNDVFDTNLIPEASAGPLSDCRVVWIITEADPSMPA
                     HEQAPAVQPGPRFGGTPAFSVPRDLGFRLVERWQFNLVQVIEATK"
     gene            complement(5847..6656)
                     /locus_tag="VFC01_35520"
     CDS             complement(5847..6656)
                     /locus_tag="VFC01_35520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HZN84967.1"
                     /translation="MDRRNMMLMAGLGALAAAIPLPKAGAAPPDAAGAYIFQDEFDGP
                     MGAAPNPANWTVQSWQDDVFPPVEGIYRDDRRNVFLDGNSNLVLLATQESGSYFSGKL
                     RGNWRVPIGHTWEARIKLDCLFPGLWPSFWAVNEDPLPDGEVDVFEWYGNGNWPPGST
                     VHAASNGKTWEGKSIPGMVDGAWHTWRMRWDEDGFKFWRDYVDGAKPYFSVPPKPIPV
                     HGNVTDLRWPFNNPGYWLSPMFTLAVGGVGAGDPAGGTFPAAFLVDYIRVW"
     gene            6781..>7678
                     /locus_tag="VFC01_35525"
     CDS             6781..>7678
                     /locus_tag="VFC01_35525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84968.1"
                     /translation="MDLVLGLSMTSADVRWVLVEGATAEGATFRRSEVDFDTLDDVIA
                     AVGDHRLHAIGVTWANAAESAASTLLDALADRGLDNVITVSEEEAAEALATGIAERGG
                     YSAVAVCIVEPDAVLVALVDADDVMTEQVEATADDVLALLDRNEAQPEAIFVLGSAED
                     IDAIATSLDGVAVPVITAAEADVALARGAALASARALNMLDARPARWRVPSRIGALSS
                     VVAAAIVVFVVSLSAALGLGLTPDEDSAVAQRDTAVTKDQPARMPAAPSVAQAPPKPI
                     PVAPPPAAPPEPAPPVAQTIVEA"
BASE COUNT         1350 a         2514 c         2487 g         1327 t
ORIGIN      
        1 tgctggccgt ggtgccgtac ttctcgaccg tcatcgggac cgtcggaatg gacctcgatc
       61 aactgcgtgc gtggttcgac cgtcccgccg cgacaacgct ccaaatcgcc gctgccccag
      121 gcgttgatcc gaagcaacta atccaggacg tccgcaacgt agtgcccgcc ccgaactacg
      181 tatacgacgg cagcacagcg ctggcaggca tggaggcgcc gctgcgtcag agcatgttca
      241 tcgccaacgc cgtgtggatc atcgtggtgt tggtcgccgc cgtcgcactt ctcaacacgc
      301 tgacgctttc tgtgctcgaa cggcgccgcg agatcggcgt gctgcgggcg atgggctcca
      361 gccgccggtt caccctggga atggtgctga ccgaagcggt gggcatcggt gtcgtcggcg
      421 gtaccctcgg cctgctgttc ggcctgaccg accagtggct ctacagcctt gtcagcggcg
      481 acatcatgaa cttcaatgtc gacttccagc cgagcccgat ggctctcgtc ttcaccgtcg
      541 gtgcgattgc ggtcagcctg ctcggctcgt tgccacccgc gcgaaaagcc gcgcggctga
      601 acatcatcga ggcagtcagc gtcgagtaga gcccggcgat gtttagaagc ctagtcgaac
      661 aagttcggtg aacagttcgg cgaagggatc cgtgacagga atggcgatcg agaaggtcaa
      721 gaacggactg atgaacgtgt tcaccgtatg cccgggcaat ccaatgctcg ccgccccgaa
      781 ccccaggttg tcttcgggat cagacccaga agtcaaaaca tttgtgaaga acggagtttg
      841 gagaacaaaa gcaacatagt gattgtcatc agggtcggca gccgatgtta tcgagaacag
      901 gccgaaattg tgggtcgaac ttatgaagcc ggggtccacc gctgaagtcg tgactcccga
      961 cgtattggcg actgtagacg gcgtcgaggc ctgctcgaac tcgcggcgcg cgaaggccag
     1021 cagtgtccac tctgcgggcg actggaccgg cgcggtgggc gcggtgttcg cgaatggcga
     1081 caggaccgcg ttgatcacca tcgacacaag gcgcgacaac gggtcggcgg gcctcggtgt
     1141 ggtggccgtg ggcgtggtcg ccttctccgc tggagcaatg ggtgacgtcg tcggctctgc
     1201 cgtgattgcg gcgacagggg ccgcagcagc cgatggcgtg gttgatgcga atgacggctg
     1261 ctccgtgtga acaacgcgga tggcgtcccg gcggcgtggc gtggcgtcgg tggtaggcgc
     1321 gttgaccgcg agtggcttcg ggctgacctt caccgatgcg tcgcccgatt cctcgcgggg
     1381 ggtgccgcga cggcttcgcc gcgcatcggt gccaggtgca tccgacgtcg ccggcgtggt
     1441 cgccgcctct gtcgacgacg acgagtcagt cgttgtgctg atgttctggc tctcggaccg
     1501 cttcaccgcg ccgggcggat ccacccgacg cgggtggtga ctctcggtgc cgggcgacga
     1561 actcgacggc gacgaaccat cagacgacga gcctttgccg gtatcgtccg gagtggccaa
     1621 cgccaccgcg ggcgtattgg cgaccgccat ccccattcca agtgcgaccg ccagcgccca
     1681 gatgccgccg atgtgctttg cgttttgcat ggtggttccc tcgctcaccc gcgctgcgtc
     1741 gactcgccct gtcctgttca cggctcacgg cgtccgaggg ttcaaatttc cccgctaacg
     1801 ccatcgtcgg tggtgatcac agtcgcgaac ccccgcaaat gaggtgtaca cggtggtggc
     1861 acgagggttc gccggatgct gcgcaatccc ccggtatcga atccagccgc ggcaatggcg
     1921 tcgacggttc ggcggttcgg gtggcaaccg gaagccagcc acgaccacgg cttggcgatc
     1981 aggtcctgga accggcccat cgcgccgtcg ccgcggacgt gctcgaggac gacaagcctg
     2041 cctccgggtg ccagcacacg gcgaatctcg ccgagggtgg cggccacatc atcgaccgag
     2101 cacaacacca gcgcgacgtg taccgagtcg aaactgttgt ccgggaacgg aattgcctcg
     2161 gcaaccccat cgacaatgtc gaccgcaatg ccgcagcgct cggcaacggc cgaagccatc
     2221 cgccgaaacg acgccaccgg ttccacggcg gcgaccgctg tcaccgcggg cggtagaaac
     2281 gccaagtcgg ttcccgggcc gacgccgatc atcaggagtc ggccggtcgc ttcactcagc
     2341 gcatctcggc gatatcggtt gtagaacagc ttgtcgaaaa cgggcatgcc gagtcgatac
     2401 acatacggga acaacggatt acggcgcgtc acctatcacc cagatccatt cggtacggcg
     2461 gatattcgtc actcatgagg gagacgtaca cggcaacccg atagcgccat ctaacaatgc
     2521 ccatcaggag gtcgaacatc ccctgatggt tcgtgccggc gaacagcaac caccccgcgt
     2581 tgatcacgca caggatctgc aggaaaggct ccatcgccca gcagagaatg atctgcggga
     2641 acgcgagcag ccaccacttg accagcaccg cccagttcgt caactgttcg ggatagtcga
     2701 cctcgaggtc gcccggatag tcgggccggg gcgccatcgt aaacggcggg tacttgtcgg
     2761 tcgtgttcat cgggaaccgg tagttcatca ctcgccacga ccagcgcaac accccgacgt
     2821 tgaaatcgaa gatcgggcgc ggataccgtc cggtgaacaa gatcgcaacg cccgcaacca
     2881 aagtcgaaaa cggatacacc aagtacagcc caatcaagat cgggtagtgc ggaacggcga
     2941 gcacgcacca cttgacgagc cagagccagc gcgagggcgc gtcgatcgcg cctcgcactc
     3001 ttaccgggtc ggtcgtcatg agctggtcgt ggccgcgagg tccctgctga catccgcgga
     3061 tatccggcga tgactcacgg aactcagaaa cgtcgtcagt gcccaggtca tgtaccggtc
     3121 cgggatcacc gccgccggcc tcagcacagc ttcaccgtgg gacacctgca gcgaggtcac
     3181 gcgcgaaacg gctgcgacgc tgcgacgcct ggtcttctcg taccagcgca acactttcgg
     3241 cagatcgccc ccctggtgat cggatagcgc cttgcacaac accatcgtgt ccagcagcgc
     3301 ctgattggtc ccctgcgcga gcgtcggcgg catcgtatgc gcggcgtcgc cgagcagggt
     3361 gagccggccg cgacccggcg ggggaatcgg atgccgaaaa tgcgggtacg gcgaatcggc
     3421 cagatcgtcg tcggtcaggg ttgcgagcaa gccatcgacc gcgtcgcacc atcccgcaaa
     3481 atgctcgcgg atcacgtcga tcggccgttc gggtctgacg aatcggtatg accagggcag
     3541 gtcgaaccac cactgcactt cggagccacc ggccggccac aggccaaggt ttccgcgctc
     3601 accgatgacg agcagtgcga cgcgcttatc gggaacggtc ggaagcgtgg caagtccctg
     3661 ccagctgcac caaccggtcg gttccgcctc acgcgcgccg acgatgtcgc gaaccttcga
     3721 gtgcaggccg tccgcgccga tcaacagatc gccctcggcc gcactcccgt cttcgaattc
     3781 gacccgcacc ccgccgtccg cactgaccac gtcgaccaca cggcggttgt atcggatccg
     3841 atcgcaggga aagccgtcca gcagccggtc tagcacgact cgccgcggga ccatttgaat
     3901 cggtgcgccc agccggtcct cgatcgtggt cagatccaag gtgacgagcg ggcgacccgt
     3961 cgacgtcagg acacgcacgc tggacagcag ctgacccgcc ccgtccatat ctacgcctag
     4021 ctgctgcagg accgtcgacc cgttggacca gatggtgacg gctccgccac cagcccgcgc
     4081 atcaggccgc cgttcgaaga cggtgacgtg gtggccatcc cgcagcaatc cgcgagcgac
     4141 ggaaatgccg cccacaccgg cgcccacgac gaggatgcgt agtggccggg taggcggcac
     4201 agctggcccg ttccgcccca cgcctcaaat ttacgacggg cgtccagccc gggtgtcggg
     4261 cttcggcgaa cggcgagggc cccggatatg acccatcgcg gttctggcac catagcggcg
     4321 tgttcgggcg gggcgcttcg aggagaccgc cgcccactgt gctggacccg gtggtcgtgg
     4381 gagtgttcgc cgccgcagtg agtctggtcg gcgcggggcg cccgtctttc tggtacgacg
     4441 aggccgcgac gatttcggcg tcatacagtc ggtcactcgg ccagttgtgg cagatgttga
     4501 acaacgtcga tgccgtccac ggcctgtact acctgctgat gcagggctgg tttcacgtcg
     4561 tcccgccgac ggagttctgg tcccgcgcgc caagcggttt ggccgtcgga gcggcagcgg
     4621 ccggagtggt ggtgctggcc aagcaattct cgactcgcac ggtcgcagtg agctcagggc
     4681 ttgtctgcgg gatattgccg cgggcgacat gggcgggcat cgaagctcgg ccctacgcga
     4741 tttcgatggc ggccgcggtg tggctgaccg tgctgctagt tcacgccacc cgccgaaaca
     4801 ccgcctggct ctggctgtcc tatggaattg ccctagctac atcgattctg ctcgacgtgt
     4861 acttggctct gatgttgttg gcgcacttcg ctttcgtctg cctccaccgg cgcgcacgaa
     4921 ctgtcctggt gcgattcgcc atcacgtccg ttctgacagg cgcagcggtg acgccgtttg
     4981 cgctcgaggt cgtcggtcag gcacatcaga tcgtctggat cgcaccggtc ggccgcagga
     5041 cgatcgaaga cgtcggggtt caacagtatt tcgaaaggag cccgccgttt ctgatcctgt
     5101 cggcgctgat tgtggcgact gctattgccc tgtggctctt cacatccgcg cgactgacgc
     5161 agccagatcg ccaactgctg acgctggcga tcgggtggat ggcgatacca acggccatga
     5221 tcctcatctg gtcggcggtg gttcagccga tctacacccc tcgctacttg tgcttcaccg
     5281 cgccggcgat cgcactggtg ttgggggtct gcatcggcgc attggctgtt agaccgtggg
     5341 cggcggcggc cgtcgtaacc gcgttggctc ttgccgcagg accgaattat cttctggtgc
     5401 aacgcggtcc gtacgcgaag tacggaatgg attacagcca ggtagccgat ctgatcaccg
     5461 cgaaggctgc gccgggcgat tgcctgctcg tcaacgacac ggtgagcttc atgcccgccc
     5521 cgatgcgacc cttgctggcg gcgcgcccag atgcctacca caagttggcc gacctcagcg
     5581 tgtggcaacg cgcgaccgac cgcaacgacg tcttcgacac caacctcata ccggaggcga
     5641 gcgccgggcc gttgagcgac tgccgcgtcg tgtggatcat caccgaagcc gacccatcga
     5701 tgccggctca cgagcaggcg ccagcagtgc aacccggtcc ccgattcgga ggcacgcccg
     5761 ccttctcggt tccgcgcgac ctggggttcc gccttgtgga acgctggcag ttcaacctgg
     5821 ttcaggtcat cgaggcaacg aaatagctac cagacgcgga tgtagtcgac caagaaggcc
     5881 gccgggaagg tacccccggc aggatcgcct gcgccgaccc cgcccaccgc gagggtgaac
     5941 atgggagaca gccaatagcc ggggttgttg aacggccacc gcaagtcggt cacattgccg
     6001 tgcaccggga tcggcttcgg cggcacgctg aagtacggtt tcgcgccgtc aacgtagtcc
     6061 cgccagaact tgaaaccgtc ctcgtcccac cgcatccgcc aggtgtgcca cgcgccgtcg
     6121 accattccgg ggatggactt tccttcccag gttttgccgt tggatgccgc gtgcacggtg
     6181 gatcctggcg gccagttgcc attgccgtac cactcgaaga cgtcgacctc gccgtcgggc
     6241 agcggatcct cgttcaccgc ccagaacgac ggccagaggc cggggaacag gcagtcgagc
     6301 ttgatgcgcg cttcccacgt gtgcccgatg ggcacccgcc agttgccccg cagtttgccg
     6361 ctgaaatagc tgcccgactc ctgcgtggcg agcaggacga gattggagtt gccgtcgagg
     6421 aacacgttac ggcgatcgtc gcggtagatc ccctcgacag gcggaaacac gtcgtcctgc
     6481 cagctctgca ccgtccagtt cgccgggttg ggggccgcgc ccatcgggcc gtcgaactcg
     6541 tcctggaaga tgtaggcacc agccgcatcg ggcggcgcag ctccggcctt gggtagcggg
     6601 atagcggcgg caagcgcacc gagcccggcc atcaacatca tgtttcgacg atccatctga
     6661 cgcacgagcg caaccctaag ggcagaagcc gtgaccgtgt gcgatttacc gacggttttt
     6721 gtgaattcga ttagagcggt agtctcggaa cctgatttat ccggatcggg agcacaccag
     6781 atggacctcg tactcggtct gtcgatgacg tcggcagacg tccgatgggt gctggtcgag
     6841 ggcgcgaccg ccgagggcgc caccttccgc cgtagcgagg tcgatttcga cacgcttgac
     6901 gacgtcatcg ccgctgtcgg ggatcaccgg ctgcatgcca tcggcgtcac ctgggcaaac
     6961 gcagccgagt ccgccgcgtc gacccttctc gacgccctgg ccgaccgtgg cctggacaac
     7021 gtcatcacgg tgtcggagga ggaagccgcc gaggcgctgg cgacaggcat cgccgagcgc
     7081 ggcggctaca gcgcggtcgc cgtctgcatc gtcgaacccg acgctgtcct cgttgctctg
     7141 gtcgatgcgg acgacgtgat gacggagcag gtggaggcga cggccgacga cgtgctcgcg
     7201 ctgctcgatc gcaacgaggc ccagcccgag gcgatcttcg tactgggttc ggccgaggat
     7261 atcgatgcga tcgcgacctc gttggacggt gtcgcggtgc cggtgatcac cgcggccgaa
     7321 gctgatgtcg cgctggcccg gggagccgcg ctggcatcgg cgcgagcgct caacatgctg
     7381 gacgcgcggc ccgcgcggtg gcgcgtgcca tcgagaatcg gcgcgctgtc gtcggtcgtg
     7441 gccgccgcga tcgtcgtctt cgtcgtctca ctgtccgcgg cgctcggcct cggcctgaca
     7501 ccagacgagg attcggctgt ggcccaacgg gataccgcgg tcacgaagga tcagccagcc
     7561 cggatgcccg cagctccgtc ggtcgcacag gccccaccga aaccgattcc ggttgcaccg
     7621 ccgcccgcgg cacccccgga gcccgcaccg cccgtcgcgc aaacgatcgt ggaggctg
//
LOCUS       DASRJA010000789        18833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3078902,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000789 DASRJA010000000
VERSION     DASRJA010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 18833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18833
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3078902"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..656)
                     /locus_tag="VFC01_35530"
     CDS             complement(264..656)
                     /locus_tag="VFC01_35530"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN84969.1"
                     /translation="MRIRHLGIVVQDLERTAEFYENVLGFKRLGDARTPGHYPGKALD
                     LSDGEVNYSLLQPNPEIERSEWTYGAMGPNHIGVTIEDTGAVVRALKDRGIEVYGAEA
                     ADPPRFFKFRDPDGVEVDVATPERGWKF"
     gene            complement(752..1690)
                     /locus_tag="VFC01_35535"
     CDS             complement(752..1690)
                     /locus_tag="VFC01_35535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZN84970.1"
                     /translation="MAEDLPDDEKALDAYSTVVTTVARAVLPSVASLAVRTSRGDGSG
                     SASVITPDGFLLTSAHVVAGANSAEAAFTDGTTVKVDVVGRDVLSDLAVLKAHDAVPA
                     PVAMGRAEDLQVGQLVVAVGNPLGLAGSVTAGIVSGLGRSLPTKAGRVVDEVIQTDAA
                     LNPGNSGGALADGRGRMVGVSTAVAGVGLGLAVPVNDSTHRIIGSLMSTGRVRRAWLG
                     IAGARIPVAPALATKLGTATGLQIASVVKGSPAAEAGLRRGDIVVSVDGHDIVTATAI
                     QKLMVEDAIARRIEMTVWRNGALVDVFVVPRELADT"
     gene            complement(1747..3048)
                     /locus_tag="VFC01_35540"
     CDS             complement(1747..3048)
                     /locus_tag="VFC01_35540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN84971.1"
                     /translation="MLIQRATLLAGTTTDIRVDQQIVDVGELTPDKGEQVLDVAGATV
                     IPGLHDHHVHLRSAAAALTSARVGPAEVRGREDLKRALAEAPVGSDGWVRAIGYHEAV
                     AGPLDRHVLDEVSPPLPVRVQHRSGVLWTLNSAGLARVGLTDHTDGRLRSADRHWSDA
                     LQRNESGLNEVSQRLSAYGVTGVTDATPDFEVSDIVKLLQAHRHGELAQRVHCLAPGK
                     RILHDSDLDLDELKDWVAERHAADAPVAVHCVTAAQLVVTLSALREAGTHLQDRIEHA
                     AVVPDGSIADLKNLGVTVVTQPNFVAERGDQYLTDVPAAEHHELWRLASLLKAGVKVA
                     LSTDMPFGEGDPWATMRAAVRRTTATGAVLGPDESVSAREALTMFFGTSGRPTEPRTI
                     TKGQPGDLCVLAASPEDVIEELDARMVNATVVAGEVVFERG"
     gene            complement(3042..4136)
                     /locus_tag="VFC01_35545"
     CDS             complement(3042..4136)
                     /locus_tag="VFC01_35545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZN84972.1"
                     /translation="MAIPVAVLTRARRAADAFQALTGVETDSVELLTGRAALLGLTPP
                     GRISAGGATHLMRSSDEWCALTLSRQDDVDAVPALLESDAVADDPWPAVQQWVATNTP
                     ADITSRARLLGLPVAALGETAPAPPRITRLGNPAAPRTPAELLVADLSSMWAGPLCGQ
                     LLARAGATVVKVETLARPDGTRAGPPKFFDWMNSEKLSYAVDFDKPAGLRRLLEAADV
                     VIESSRPAALRHRGLGPMPRDGRVWLRITGHGADGERANWVAFGDDAAASGGLVSGTS
                     EPQFCGDAIADPLTGLHAAQHVAESLSRGGGELIELSMAAVAATYAQDDRTVETSCAA
                     TPAIASRAPALGADNAAVERLLAERRLASC"
     gene            complement(4159..5148)
                     /locus_tag="VFC01_35550"
     CDS             complement(4159..5148)
                     /locus_tag="VFC01_35550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZN84973.1"
                     /translation="MATSAAVSGPYFDDLEVGQVFDSAPSMTLSQGAAGAHQAIIGDR
                     LRLALDAQLAYAVTGATSALAHPALVCDVAIGQSTLATLRVKANLFYRGLEFHRFPVI
                     GDSLFTRTEVVGLKQNSAKPGRAPTGLAALRMTTVDQIGRLVLDFYRCAMLPLSAEHE
                     TGRNDDLTNIGADLAAPPDPTADWDADVYREKVPGAHFDPGLAGAVLQSTADVVTSAP
                     ELARLTLNVAATHHDWRVGGQRLVYGGHTIGLALAQTTRLLPNLVTVLGWESCDHLGP
                     VHEGDTLHSELHVEAADPLPDGRGGVLKLRSLVFAVGEPDRKVLDWRFSALQF"
     gene            5182..6357
                     /locus_tag="VFC01_35555"
     CDS             5182..6357
                     /locus_tag="VFC01_35555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN84974.1"
                     /translation="MIEEETLLVETVRAFIDRDVKPTVREVEHANEYPEAWIEQMKRI
                     GIYGLAIPESYGGSPVSTRCYVLVTQELSRGWMSLAGAMGGHTVVAKLLSLFGTKEQK
                     RQYLPPMATGELRATMALTEPGGGSDLQNMKTTALSAGDGLVINGSKTWISNARRSGL
                     IALLCKTDPTATPAHKGISVVLVEHGEAAPGLDIGSSPGLSISRDLPKLGYKGVETCE
                     LTFDDCRVPASAILGGEPGKGFAQMMKGLETGRIQVASRALGVATAALEDALRYAQER
                     ESFGQPIWKHQAVGHYLADMATKLTAARQLTLYAADRYDAGERADMEAGMAKLFASEV
                     AMEIALNAVRIHGGYGYSTEFDVERYFRDAPLMIVGEGTNEIQRNVIASQLVARGGI"
     gene            6357..7079
                     /locus_tag="VFC01_35560"
     CDS             6357..7079
                     /locus_tag="VFC01_35560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN84975.1"
                     /translation="MKPAYQALREQLRGEIAAGHYRDGARLPTESELVARHGLSRQTV
                     RRAFQDLVAEGMVYRVPGRGTYASEPGRRYLRQLGSIEDLMSLSDDTTMEVLTGLRRR
                     VDVDAASRLRLDDDVVYSVVFRRLHDGVPFVLTTVHLVPSEAQPVLDSPALADGAVGT
                     HTVIGLLEPHLTEPIAEAAQSITVALADGIVADAVGCQPGHPMLRVDRLYSDSGGRPV
                     ELSVSHFLPEQYTYRVTLRRSG"
     gene            complement(7049..8005)
                     /locus_tag="VFC01_35565"
     CDS             complement(7049..8005)
                     /locus_tag="VFC01_35565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZN84976.1"
                     /translation="MGLPSMRPTGWFQVAWSADLEVGDVKPLHYFGTELVAFRDLDDI
                     VRVLDAHCQHMGADLSHGGCVVEGGIQCPFHGWVWSGDDGHNVRIPYEPHADDARRVH
                     CWPTAEFNECIYVWHDVRHRAPLWPAPDGWGGLGERVSSYWFRAVGPDERQFFAGVTV
                     HPQVVAESSVDRQHYRFVHKVPVSPILLASTADNSSWHATIGFGAGNPVEICWHGIGL
                     SFSCEDNGDGMQIISICPTPVDDTTTDIFATYWVSEDLDYDERLAAAKRMLPDDIEIW
                     AHLQYLERPALAPSEADDFQHMRQWARSFYPPAQPDLRSVTL"
     gene            complement(8088..8963)
                     /locus_tag="VFC01_35570"
     CDS             complement(8088..8963)
                     /locus_tag="VFC01_35570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN84977.1"
                     /translation="MTELASVVVAVGSPSDDATFTLTEDDCDDRRAITVPSVPEALAE
                     LTERCQRWPQAAAVCDDVLRSIDPAAPTRAGVITESLAYSTLQSGPEFARWLAERGPP
                     TVPDISDPVQADRDDSRLHVRFNRPQRHNAFSTDMRAELLDALEVARLDPSITEVVLT
                     GNGPSFCSGGDLAEFGTFADPASAHLARTRYSPALVLDELTARLGRNCRAEVHGKVLG
                     SGLEMAAYCGWVQAHPDAVLGLPELSLGLIPGAGGTVSITRRIGRWRTAYLVLSGQTI
                     DTDTALRWGLVDEVQ"
     gene            complement(8960..10471)
                     /gene="fadD4"
                     /locus_tag="VFC01_35575"
     CDS             complement(8960..10471)
                     /gene="fadD4"
                     /locus_tag="VFC01_35575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase FadD4"
                     /protein_id="HZN84978.1"
                     /translation="MQIREHAEANPDKPAIIIHPSGTVLTFAELEARANRLAHFFRKH
                     GLREGDAVAILMENNQHFHAVMWAARRCGLYYVPINTHLTAAEAAYIIDNSNAKAVVG
                     SAALRKTCENLAEHTQPDLRIIADDDLDGWHRYPECVADQPDTPIDDEIEGDLLQYSS
                     GTTGRPKGIKRELPHLPPAEVPGLMSALVGFWLDPEAVYISPAPLYHTAPSVWSMQIQ
                     GAGITTVVLEKFDAEGCLDAIQRYRVTHGQFVPVMFTRMLKLPESVRNKYDLSSMKRV
                     MHAAAPCPVEIKKQMMDWWGPIVDEYYASSEAIGSTLITAEDWLAHPGSVGKPMMGGL
                     HILDEDGNELPPGQAGEIYFEGGFDFEYLNDPEKTAKSRDSHGWKTVGDIGYLDEEGY
                     LYLTDRRHHMIISGGVNIYPQEAENMLVTHPKVMDAAVFGIPDDEMGQSVKGVVQTVD
                     PADATDEFAEELLVWLRDRLAHYKCPRSISFEAQLPRTDTGKLYKQELINKYA"
     gene            complement(10620..12446)
                     /locus_tag="VFC01_35580"
     CDS             complement(10620..12446)
                     /locus_tag="VFC01_35580"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HZN84979.1"
                     /translation="MTAATIPGLDTAPTKHKGLLAWVQEVAELTQPDRVAFADGSEEE
                     FERLAAHLVDAGTFKRLNDEKLPNSYLALSDPSDVARVESRTFICSEREIDAGPTNNW
                     MDPAEMRGIMTDLYRGSMRGRTMWVVPFCMGPLGAEDPKLGVEITDSEYVVVSMRTMT
                     RMGQAALDKIGDDGFFVKALHSIGAPLEPGQKDVPWPCNDTKYITHFPETREIWSYGS
                     GYGGNALLGKKCYSLRIASAMAHDEGWLAEHMLILKLISPENKSYYVAAAFPSACGKT
                     NLAMLQPTIPGWRAETVGDDIAWMRFGKDGRLYAVNPEFGFFGVAPGTNWKSNPNAMK
                     TIEAGNTVFTNVALTDDNVVWWEGLEGDPQHLIDWKGREWTPDSDEKAAHPNSRYCTP
                     MSQCPTLAPEWDDPQGVPISAILFGARRKTTVPLVTQARDWQHGVFIGATMGSEQTAA
                     AEGKVGTVRRDPMAMLPFLGYNVGDYFAHWIDIGKQSDESKMPKIFFVNWFRRGDDGR
                     FLWPGFGENSRVMKWIIDRIEQRAGGKTTPIGTVPTAEDLDLEGLDVDAADVDAALAV
                     NAAEWRNELPEIEEWFEFVGEKLPTGIKDEFDALKHRLAEAD"
     gene            complement(12707..14137)
                     /locus_tag="VFC01_35585"
     CDS             complement(12707..14137)
                     /locus_tag="VFC01_35585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84980.1"
                     /translation="MSDPIASADALVAAIEPGVSSPGVVTRDVVLVTGPWLAGTSSLL
                     AALRQRLPDVTFVETGELSVVEAPIAVVFIVSAVARLTESDCALLDLAAANTDLVIGV
                     VSKIDVHRNWRNVLAADRQTLIAHDARYRDVPWVGVAAAPDLGDSKIDELAERLGHLG
                     DARLARRNRLRAWDTRLQTAVRRLEEEAAGVTRNARIADLQEQRSATQRDRRLAKTER
                     TITLRSQVQHARLQLSYFARNRCTSVRAELQEDVAELGRRRLGEFEPYVRTRVDEVVA
                     EVDEGITTQVGDLATELELSTPPPPPSPMPPPEVSPPPLKSRRLETQLMMILGAGFGL
                     GVALMVTRLFAGLAPGLTVAGLAAGGLVGLLLTVWVVGIRGLLHDRAMLDRWVTDVTA
                     ALRSALEERVATRVLAAETALTSELATRDELESATAADRVAKIDAELHEHAIATARAT
                     TVRDRRLPLLQKAIEAVRAELYGATM"
     gene            complement(14134..15120)
                     /locus_tag="VFC01_35590"
     CDS             complement(14134..15120)
                     /locus_tag="VFC01_35590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84981.1"
                     /translation="MEGHQIFVDALTRFADQSRNPRLTPIIRRIAAPLRIAVLGRDGV
                     GRDTVAAALTAAGVTGTADPASADVRVLVIAEALKPEDRGHLANADRPTLTVLNKADL
                     AGFGTGGPLALAHRRAADYQALTGVPTVPMVALLAKADLDDELVAALRTMVTVPADLT
                     STDAFVQSDHPLPQAIRARLLDTLDRFGIAHGVMAVAQGADDAKVLTLLRGLSQIDRV
                     VAQLDAVGAPVRYRRVRSAITELRALAVQFEDEGLTEFLSADETVIAVMAAAVDVVEA
                     AGIAVDGDNEAESHLRRAVHWRRYGRGPVDALHRSCGAAISRGSLRLLGSVR"
     gene            15290..16012
                     /gene="trmB"
                     /locus_tag="VFC01_35595"
     CDS             15290..16012
                     /gene="trmB"
                     /locus_tag="VFC01_35595"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HZN84982.1"
                     /translation="MQTPRPDVAEPHLHPRVTSFRTRRSTLSGGQQATWERLWPEMGM
                     HARDADGPAPQLDTEAWFGRSAPVVLEIGCGSGTSTLAMAQAEPHLDVVAVEVYRRGL
                     AQLLSAIDREGVTNIRLVRGDGVDVLEHMFEPDSLTGVRVFFPDPWPKARHHKRRLLQ
                     PDTVALIADRLRAGGVLHAATDHSGYAEQIAEVGDAEPRLRRVTASDSLPISVQRPVT
                     KYEGKAQHVGSAVAELLWEKRP"
     gene            16009..16695
                     /locus_tag="VFC01_35600"
     CDS             16009..16695
                     /locus_tag="VFC01_35600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZN84983.1"
                     /translation="MSLTEDIPHIEDAPPPVAPAEATARVLLVWDAPNLDMGLGSILG
                     GRPTAAHRPRFDALGRWLLGRTAALSAERPDVTLEPEATVFTNIAPGSADVVRPWVEA
                     LRNVGFAVFAKPKIDEDSDVDSDMLNHIALRRSEGLAGVVVASADGQAFKQPLEEIAR
                     EGVPVQVLGFREHASWALASDTLEFVDLEDIPGVFREPLPRIGLDSLPEQGAWLQPFR
                     PLSSLLTSRV"
     gene            16741..>18833
                     /locus_tag="VFC01_35605"
     CDS             16741..>18833
                     /locus_tag="VFC01_35605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZN84984.1"
                     /translation="MFAWWGRTVYRYRYIVIGVMVALCLGGGVFGISLGSHVTQSGFY
                     DDGSQSVHASVIADKAYGRDTSGHIIGVYTAPEGKTVDDPDFKQKILDNLKKVEEDHP
                     DEILRSIGYFKSPDVLKNMSDADKQHAFMSIQLKGDNDDAILNNYKKVKDDLLKVKDD
                     GVDFRMAGLQPLAAELTGTIGDDQRRAEVAAIPLVCVVLFFVFGGVVAAALPGVIGGL
                     TIAGALGIMRLVAEFAPVHFFAQPVVTLMGLGIAVDYGLFMVSRFREEIAEGYDTEAA
                     VRRTVMTSGRTIMFSAVILVASSVPLLLFPQGFLKSITYAIIASVMLAAILSITVLAA
                     ALAILGPNVDALGVRTLLRVPFFRNWKPMRIYLTWLADKTQKTKTREEVEKGFWGKLV
                     NRVMKRPAAFAVPIIIVLIALIIPLGQLALGGISEKYLPPDNSVRQAQQEFDKIFPGF
                     RTEPLTIVMESDNGQPITDQQVAEVRNKAMQISGFTDPDNDPSKMWQARPYLDGASKQ
                     PSIRVIQNGLVNRNDAAKKIEELRAMTPPRGITVSVGGTPALEQDSIHSLFDKLPLMV
                     VLLITTTTILMFLAFGSVVLPIKAAVMSALTLGSTMGVLTWMFVDGHGSGLMNYTPQP
                     LMAPMIGLIIAVIWGLSTDYEVFLVSRMVEARERGMSTAEAIRIGTATTGRLITGAAL
                     VLAVVAGAFVFSDLV"
BASE COUNT         3100 a         6176 c         6324 g         3233 t
ORIGIN      
        1 cgccgcggcc ccgatggcga ggccgaagcc gagcaggatc gcggggtggt gccgctcggt
       61 ccaccggccg aagcccaccg cgagcacgat cggcggaacg gcgaagcatg cggccgtcac
      121 gccgaccgtg gcgccgtcag cgccgagcgc aaggagccgg tacgtcgaca ccggccgggc
      181 taatgtgacc gcgacctgaa gcaggaagat gccggcgacg gtgccgacga gccacagcgg
      241 cggaagcgag cggcgctggc cagtcagaac ttccagccac gctccggcgt ggcgacgtcg
      301 acctcgacac cgtcgggatc gcggaacttg aagaaccgcg gcggatctgc tgcctcggcg
      361 ccgtacacct cgatgccccg gtccttcagc gcgcggacca cggcgccggt gtcttcgatc
      421 gtcacgccga tgtggttggg ccccatcgcg ccgtacgtcc actcggaccg ttcgatttct
      481 ggattcggct gcagcagcga gtagttcacc tcgccatcgc tgagatcgag tgccttgccg
      541 gggtagtgcc cgggcgttcg ggcgtctccc agacgcttga agcccaggac gttctcgtag
      601 aactcggcgg tccgctcgag gtcctggacc acgattccga ggtgtctgat gcgcactgtt
      661 ctctccctgt gctggttgtc ttccgtactg aggaaactat ttctgcaaat cagactgtag
      721 gcgtgcaggg tggaatcagt caatggacac gtcaggtatc ggcgagctcg cggggcacca
      781 cgaacacgtc gaccagcgcg ccgttgcgcc acaccgtcat ctcgatgcgg cgggcgatcg
      841 cgtcctcgac catcagcttc tgaatcgcgg tcgcggtgac gatgtcgtgg ccgtcgacgc
      901 tgaccacgat gtcgccgcgg cgaagacccg cctcggccgc ggggctgccc ttcacgacgc
      961 tggcgatctg cagtccggtg gcggtgccga gcttggtcgc cagcgccggt gcgacgggga
     1021 tgcgcgcacc cgcgatgccc agccaggccc gtcgcacccg gcccgtgctc atcagcgacc
     1081 cgatgatcct gtgcgtggag tcgttgaccg gaactgcaag ccccagaccg actcccgcga
     1141 ccgccgtgct gacgccgacc atccggccgc gtccgtcggc gagcgcgccg ccgctgttgc
     1201 cggggttgag cgcagcgtcg gtctggatca cctcgtcgac cacccggccc gctttcgtcg
     1261 gcaatgacct gccgaggccg gacacgatgc ctgcggtaac gctgccggcc aggccgagcg
     1321 gattgccgac cgccaccacg agctggccca cctgcaggtc ctccgcgcgt cccatcgcga
     1381 ccggcgcggg cacggcgtcg tgcgccttca gcacggcgag gtcggacagc acgtcgcggc
     1441 cgaccacgtc gaccttcacg gtggtgccgt cggtgaatgc cgcttccgcg ctgttggccc
     1501 cggcgacgac gtgggcactg gtcagcagga agccgtcggg tgtgatgacg ctggcgctgc
     1561 cgctgccgtc accccgtgac gtgcgcaccg cgaggctggc gacgctgggc agcaccgcgc
     1621 gggcgacggt ggtgacgacg gtggagtatg cgtcgagcgc cttttcgtcg tcgggcaggt
     1681 cttcggccat aactaattga gcgcccgcgt tgcgggggct atgccgtggt tcgcggtggg
     1741 cagaactcag ccgcgctcga aaacgacctc gcctgcgacg acagttgcgt tgaccatccg
     1801 ggcatcgagc tcttcgatca cgtcttccgg tgacgcggcc aagacgcaca ggtcgccggg
     1861 ctggcccttg gttatggtgc ggggttcggt cggtcggcca gaggtgccaa agaacatcgt
     1921 caaagcctca cgcgccgaga cactttcgtc ggggccgagg acggcgcctg tcgcggtggt
     1981 tctgcggacg gcggcgcgca tcgtcgccca cggatcgccc tcgccgaacg gcatgtcggt
     2041 ggacagcgcg accttcacgc ctgccttcag cagtgaggcc agccgccaca actcgtggtg
     2101 ttcggcggcg gggacgtcgg tcaggtattg gtcgccccgc tcggcgacga agttgggctg
     2161 ggttacgacg gtgacgccga ggttcttgag atcggcgatg ctgccgtcgg gcacgacggc
     2221 cgcgtgttcg atgcggtcct gcagatgggt gccggcctcg cgcagcgccg acagcgtcac
     2281 gaccagctgg gcggcggtca cgcaatgcac cgcgacgggg gcgtccgcgg cgtggcgttc
     2341 ggcgacccaa tctttgagtt cgtcgaggtc gaggtcgctg tcgtgcagga tgcgcttgcc
     2401 cggtgccagg cagtgcacac gctgggccag ctcgccgtgc cggtgtgcct ggagcagctt
     2461 aacgatgtcg ctgacttcga agtcgggcgt ggcatcggtg acgccggtga cgccgtatgc
     2521 gctcagtctc tggctgacct cgttgagccc gctctcgttg cgctgcaacg catctgacca
     2581 gtggcggtcg gcgctacgca gccgcccgtc ggtgtgatcg gtcagcccca ctctggccag
     2641 ccccgccgag ttcagcgtcc acagcacgcc gctgcggtgc tgcacgcgga ccggtagcgg
     2701 cggcgacact tcgtcgagca catggcggtc cagcggtccc gcgaccgcct cgtgatagcc
     2761 gatggccctg acccagccgt cactgccgac gggcgcttcg gccagcgcgc gcttcaggtc
     2821 ctcgcggccc cttacctctg cgggcccgac gcgggccgac gtcaaggcgg ctgcggccga
     2881 ccgcaaatgg acatggtgat cgtgcaagcc ggggatgacc gtcgcgcccg cgacgtcgag
     2941 gacctgctcg cccttgtccg gcgtcagctc accgacgtcg acgatctgtt ggtcgacccg
     3001 gatgtcggtg gtggtgccgg cgagcagcgt ggcgcgttga atcagcatga tgccaaccgt
     3061 ctttcggcga gcagccgttc gaccgcggcg ttgtccgcac cgagcgccgg ggccctggag
     3121 gctatggcgg gcgttgcggc gcaactggtt tcgacggtcc gatcgtcctg ggcataggtt
     3181 gcggcgacgg cggccatcga gagttcgatc agctcaccgc cgccgcggct cagcgattcc
     3241 gcgacatgtt gcgcagcgtg caggccggtc aacggatcgg cgatcgcgtc gccgcagaac
     3301 tgtggctcac tggtaccgct gaccaagccg cccgatgctg cggcgtcgtc gccgaacgcc
     3361 acccagttgg cgcgctcgcc gtcggcaccg tggcccgtga tccgcagcca gactcgcccg
     3421 tcgcgcggca tcgggcccag accgcggtgc cggagcgctg cgggccgcga cgactcgatc
     3481 acgacgtcgg cggcctccag cagccgtcgc aggcccgcgg gtttgtcgaa atcgactgca
     3541 taggagagct tttcgctgtt catccagtcg aagaactttg gcggcccggc gcgggtcccg
     3601 tcgggccggg cgagagtttc gaccttgact acggtcgcgc ccgcgcgggc cagcagctgt
     3661 ccgcacaggg ggcctgccca catcgacgag aggtcggcga ccaacagttc ggctggcgtg
     3721 cgcggggcgg ccggattgcc cagccttgtg attcgcggtg gggcgggagc tgtttcgccg
     3781 agggcggcga cgggaagacc gagcaggcgc gcccgcgagg tgatgtcggc gggggtattg
     3841 gtggccaccc actgttgcac agccggccag ggatcgtcgg cgaccgcatc ggactcgagt
     3901 agtgcgggaa cggcgtcgac gtcgtcttgc cgcgacaacg tgagcgcgca ccattcgtcg
     3961 ctgctgcgca tcaagtgggt ggccccgccc gcggaaatcc ggcctggcgg cgtcagtccg
     4021 agtagcgcgg cccgaccggt gagcagttcg acggagtccg tctcaacgcc ggtgagggct
     4081 tggaacgcgt cggcggcccg ccgcgcccgt gtcagcacgg cgacaggtat ggccatcggc
     4141 gtcacacgct cattgtgctc agaactggag cgccgagaac cgccagtcca gcactttgcg
     4201 gtcgggctcg cccaccgcga acaccagcga gcgcagtttc agcactcccc cgcggccatc
     4261 gggcagcggg tccgcggcct cgacgtgcag ttcgctgtgg agcgtgtcgc cctcgtgaac
     4321 cgggcccaaa tggtcgcagg attcccagcc gagcaccgtg accagattgg gcagcagccg
     4381 cgttgtctgc gccaaggcca gcccgatggt gtgcccgccg tagacgagcc gctgaccacc
     4441 gacgcgccaa tcatggtgtg tggcagcgac gttcagcgtc agccgggcga gctccggggc
     4501 gctcgtgacc acgtcggcgg tgctttgcag aactgcgccg gcgaggccgg ggtcgaaatg
     4561 ggcacccggc accttctcgc ggtatacgtc ggcgtcccag tcggcggtcg ggtcgggcgg
     4621 cgccgcgagg tccgcaccga tgttggtcag gtcgtcgttg cgaccggtct cgtgttcggc
     4681 gctcagcgga agcatcgcgc agcggtagaa gtccagcacc agcctgccga tctggtcgac
     4741 ggtggtcatc cgcaacgccg caagccctgt cggcgcacga cctggcttgg ccgaattctg
     4801 cttcaacccg acgacctcgg tgcgggtgaa caggctgtcg ccgatgacgg ggaaccggtg
     4861 gaactccagc ccgcgataga acaggttggc cttcacccga agggtggcca gcgtgctctg
     4921 tccgatcgcg acgtcgcaga ccagcgcggg gtgtgccagc gcggaggttg ccccggtcac
     4981 ggcgtacgcc agctgagcgt cgagtgcgag ccgcagacgg tcgccgatga tcgcctggtg
     5041 cgctcccgcg gcgccttggc tcagggtcat cgagggtgcc gagtcgaaga cctggccgac
     5101 ctccaagtcg tcgaaatatg ggccgctcac ggcagccgat gttgccattt gggcgtacaa
     5161 agtgtcaata tggccgtaca aatgattgaa gaagaaacac tgctggtcga gacggtgcgg
     5221 gcgttcatcg accgggatgt gaagccgaca gtccgcgagg tcgagcacgc caacgagtac
     5281 cccgaggcgt ggatcgagca gatgaagcgc atcgggatct atgggctggc gatccccgag
     5341 tcctacggcg gctcgccggt gtcgacacgg tgctacgtgc tggtcaccca ggagctgtcg
     5401 cgcggctgga tgagcctggc aggcgcgatg ggcgggcaca ccgtcgtcgc gaagctgttg
     5461 agcctgttcg ggacgaagga gcagaagcgc cagtatctgc ccccgatggc gaccggtgaa
     5521 ctgcgggcca cgatggcgct gaccgagccc ggcggcggct cggatctgca gaacatgaag
     5581 accaccgcgc tgtccgccgg cgacggtttg gtgatcaacg ggtcgaagac gtggatcagc
     5641 aatgcgcgcc ggtccggtct tatcgcgctg ttgtgcaaga ccgatccgac ggccacgccc
     5701 gcgcacaaag ggatttcggt ggtgctcgtc gagcacggag aggccgcccc gggcctcgac
     5761 atcggctcca gcccgggact ttccatctcg cgcgatctgc ccaagctggg ctacaagggc
     5821 gtcgaaacct gcgagctgac gtttgacgac tgccgggtgc cggcgtcggc gatcctcggc
     5881 ggtgagccgg gcaagggctt cgcgcaaatg atgaaaggcc ttgagacagg ccgcattcag
     5941 gtggcctcgc gagctctggg ggtcgcgaca gcagcactcg aggatgcatt gcgctacgca
     6001 caggagcgag agagcttcgg acagccgatc tggaagcacc aggccgttgg ccattacctg
     6061 gccgacatgg cgaccaagtt gactgccgcc cgtcagctca cgctgtacgc agcggaccgc
     6121 tacgacgcgg gcgagcgcgc cgacatggag gccggcatgg ccaagctgtt cgcctccgag
     6181 gtcgcgatgg agatcgcatt gaacgcggtc cgaatccacg gcggctacgg ctattccacc
     6241 gaattcgacg tcgaacgtta cttccgggat gcgccgctga tgatcgtcgg cgaaggcacc
     6301 aacgagatcc agcgcaacgt gatcgccagt caattggtgg cccgcggagg catctgatga
     6361 agcccgcgta tcaggcgctg cgtgaacaac tccgcgggga gatcgctgcg ggccactacc
     6421 gcgacggcgc gcggctgccc accgaatctg aacttgttgc acggcatggt ctctcgcggc
     6481 agactgtgcg gcgggcgttt caggatctgg tagccgaggg catggtgtat cgggttccgg
     6541 gtcgcggtac ttatgccagc gagccgggcc gacgttatct gcgccagctc gggtcgatcg
     6601 aagatctgat gagcctgtcc gacgacacca cgatggaggt gctgaccggg ctacgccgcc
     6661 gcgtcgatgt cgatgctgcg agccggctgc ggctggatga cgacgtcgtg tactccgtgg
     6721 tgttccggcg gctgcacgac ggggtgccgt tcgtgttgac cacggtgcat ctggtgccgt
     6781 cggaggcgca gccggtgctg gactcgcctg cattggccga cggtgccgtc ggaacgcata
     6841 cagtcatcgg actgctggaa ccgcatctga cggagccgat cgctgaagcg gcacaatcga
     6901 ttacggtcgc gcttgccgac ggaatcgttg ccgatgcggt gggctgtcag ccgggccatc
     6961 caatgctaag ggtggaccgg ctgtattcgg attcgggcgg caggccagtc gagttgtcgg
     7021 tcagccactt cctgcccgag caatacacct acagggtgac gctgcgcagg tcgggctgag
     7081 cgggcggata gaagctgcgg gcccactgcc gcatgtgctg gaagtcgtcg gcctccgatg
     7141 gcgccaacgc gggacgttcg aggtattgca agtgcgccca gatttcgatg tcgtcgggca
     7201 gcatacgttt ggctgcggct aaccgttcgt catagtcgag gtcttcggac acccagtagg
     7261 tcgcgaagat atccgtagtc gtgtcatcca caggtgtggg gcagatcgag atgatctgca
     7321 tgccgtcgcc gttgtcctcg cagctgaagc tcaaaccgat tccatgccaa catatttcga
     7381 ctggattgcc ggcgccgaag ccgatggtgg cgtgccagct ggagttgtct gcggtcgagg
     7441 cgagcaggat cgggctcacc ggcaccttgt gtacgaatcg gtagtgctgg cggtcaaccg
     7501 agctttcggc aaccacctgc ggatgcaccg tcacgcctgc gaagaattgc ctctcatccg
     7561 ggcccactgc acggaaccag tagctgctga ccctctcacc caatccgccc cacccgtcgg
     7621 gtgcgggcca gagcggcgcg cgatgccgta cgtcgtgcca gacgtagatg cactcgttga
     7681 attcggcggt aggccagcag tgcacgcgcc tggcgtcgtc ggcgtgcggc tcgtacggga
     7741 tccggacgtt gtggccgtcg tcaccgctcc acacccagcc gtggaacggg cattggatgc
     7801 cgccttcgac gacgcagccg ccgtgcgaga ggtcggcgcc catgtgctgg cagtgcgcgt
     7861 cgaggacgcg gacgatgtcg tcgaggtcgc ggaaggccac gagctccgtg ccgaagtagt
     7921 gcaggggttt aacgtcgccg acctcgagat cggcgctcca ggccacctgg aaccagccgg
     7981 tgggtcgcat gctgggcaac cccatgctgc cagcctgcca cttatgcgat ttcggcgcgg
     8041 aaacatgcgc tcagcgacca cgctcagcga ccgtttgcgc accgaaatca ctgcacttcg
     8101 tcgacgaggc cccaccgcag cgctgtgtcc gtgtcgatcg tctggccgga cagcaccaga
     8161 taggcagtgc gccatcgacc gatgcggcgg gtgatgctga cggtgccgcc tgcgccgggg
     8221 atcaggccga gactcagttc gggcaggccg agcacggcgt cgggatgcgc ctgcacccat
     8281 ccgcagtagg cggccatctc cagcccgctg cccaacacct ttccgtgtac ctcggcgcgg
     8341 cagttgcggc cgaggcgggc ggtcagctcg tcgagcacca gtgcgggcga gtagcgggtg
     8401 cgggcgagat gcgcgctggc ggggtcggcg aaggtgccga actcggccag gtcgccgccg
     8461 ctgcagaacg atgggccgtt gccggtcagc accacttcgg tgatcgacgg gtcgagccgc
     8521 gcgacttcaa gcgcgtcgag cagttcggcg cgcatgtcgg tggaaaacgc attgtgccgc
     8581 tgcgggcggt tgaaccggac gtgcagacgg ctgtcgtcgc ggtcggcctg cacgggatcg
     8641 gaaatgtcgg ggacggtggg gggcccgcgt tcggcgagcc agcgcgcgaa ctccggtccc
     8701 gactgcagtg tcgagtaggc cagcgattcg gtgatgacgc cggcgcgggt cggtgcggcg
     8761 ggatcgatgg aacggaggac gtcgtcgcag acggccgcgg cttgcggcca gcgttggcat
     8821 cgctcggtga gctcggcgag cgcttcggga accgacggca cggtgattgc gcgacggtcg
     8881 tcgcagtcgt cttcggtcag tgtgaaagtc gcgtcgtcgg aaggcgaccc gacggcgacc
     8941 acgacactcg cgagctccgt cacgcgtact tgtttatcag ctcctgcttg tacagcttgc
     9001 cggtatcggt gcgcggcagc tgcgcctcga acgagatcga gcgcggacat ttgtagtgcg
     9061 ccaaccggtc tcgcagccag accagcagtt cctcggcgaa ctcgtcggtg gcatcggctg
     9121 ggtctactgt ctgaacgacg cccttgacgc tttggcccat ttcgtcgtcc gggatgccga
     9181 acaccgcggc gtccatcacc ttgggatggg tgacgagcat attctcggcc tcctgcgggt
     9241 agatgttcac cccgccggag atgatcatgt ggtggcgccg gtcggtcagg tagaggtagc
     9301 cctcttcgtc gagatagccg atgtcgccga ctgttttcca gccgtggcta tcgcgtgatt
     9361 tggcggtctt ctccggatcg ttgaggtatt cgaagtcgaa gccgccctcg aagtagatct
     9421 ccccggcctg gcccggtggc aactcgttgc cgtcctcgtc caggatgtgc agcccgccca
     9481 tcatcggctt gccgaccgaa ccggggtggg ccagccagtc ttcggcggtg atcagtgtcg
     9541 agccaatcgc ctcggaggag gcgtagtact catcgacgat cgggccccac cagtccatca
     9601 tttgtttctt gatctcgacg gggcagggcg ccgctgcatg catcacgcgc ttcatgctgg
     9661 acaagtcgta cttattgcga acggattcag gcagtttcag cattcgggtg aacatcacag
     9721 gcacgaattg cccgtgagtg acgcggtaac gctggatcgc gtccagacaa ccctcggcgt
     9781 cgaacttctc cagcactacg gtcgtgatgc cggcgccctg aatctgcatc gaccacaccg
     9841 aaggcgcggt gtggtacagc ggggcggggc tgatgtagac ggcctcgggg tcgagccaga
     9901 agccgaccaa cgccgacatc aggccgggaa cttcggccgg cggcaggtgc ggaagttcgc
     9961 gcttgatgcc cttggggcgg ccggtggtgc ccgacgagta ctggagtaag tcgccttcga
    10021 tctcgtcgtc gatcggcgta tcgggttgat ccgcaacgca ttcgggatag cggtgccagc
    10081 cgtcgaggtc gtcgtcggcg atgatcctta ggtcgggctg cgtgtgctcg gcgaggttct
    10141 cacacgtctt gcgcagtgcc gccgagccga cgacggcttt ggcgttgctg ttgtcgatga
    10201 tgtaggccgc ctcggctgcg gtcaggtggg tgttgatcgg gacgtagtag agaccgcagc
    10261 gacgcgccgc ccacatgacg gcgtggaagt gctggttgtt ctccatcaaa atggcgacgg
    10321 cgtcgccctc gcgcagaccg tgcttgcgga agaagtgggc gaggcggttg gcgcgcgcct
    10381 cgagctcggc gaaggtcagg accgtcccag acggatggat gatgatggcc ggcttgtcgg
    10441 ggttggcctc ggcatgctcg cgaatctgca tgggctgact ttacgcggcc gaacttgaca
    10501 cgtgtcaaga gggtcgatct tgttgccgac aagacgtaaa agtgcccaaa atccaaagga
    10561 tttgggcact tttacgttcc ccagcttgcg cgggggccct gttcgcgggt gaagacgagc
    10621 taatcggctt cggccaaccg gtgcttcagc gcgtcgaact cgtccttgat gccggtcggc
    10681 agcttctcgc cgacgaactc gaaccactcc tcgatttcgg gcagctcgtt acgccactcc
    10741 gcagcgttga ccgccagcgc ggcatccacg tcggcggcat cgacatcgag accctcgaga
    10801 tccagatcct cggccgtcgg cacggtaccg atcggcgtcg tcttgccgcc ggcccgctgc
    10861 tcgatccggt cgatgatcca cttcatcacg cggctgttct caccgaagcc cggccacagg
    10921 aagcggccgt catcgccgcg gcggaaccag ttgacgaaga agatcttcgg catcttcgac
    10981 tcatcggact gcttgccgat gtcgatccag tgcgcgaagt agtcgccgac gttgtagccc
    11041 aggaacggca gcatcgccat cgggtcgcgg cgcaccgtgc cgaccttgcc ctcggccgcg
    11101 gccgtctgct cggagcccat cgtcgcgccg atgaacaccc cgtgctgcca atcgcgggcc
    11161 tgcgtcacca gcggcaccgt cgtcttgcgc cgggcgccga acaggatcgc cgagatcggc
    11221 acgccctgcg ggtcatccca ctccggtgcc agcgtcgggc actgcgacat cggcgtgcag
    11281 taccgcgaat tcggatgcgc ggccttctcg tccgagtcgg gcgtccactc gcggcccttc
    11341 caatcgatca gatgctgggg atcgccctcc agcccctccc accacacgac gttgtcgtcg
    11401 gtcagcgcga cgttggtgaa gacggtgttg cccgcttcga tggtcttcat agcgttcggg
    11461 ttcgacttcc agttggtgcc cggcgcgacg ccgaagaagc cgaactcggg attcaccgca
    11521 tacagccggc cgtccttgcc gaaccgcatc cacgcgatgt cgtcgccaac ggtctctgcg
    11581 cgccagccgg ggatcgtcgg ctgcagcatc gcgaggttgg tcttaccgca cgccgacggg
    11641 aacgcggccg cgacgtaata cgacttgttc tccggcgaga tcagcttgag gatcagcatg
    11701 tgctcggcga gccagccttc gtcgtgcgcc atcgccgagg cgatccgcag cgagtagcac
    11761 ttcttgccca gcagcgcgtt gccgccgtag cccgagccgt agctccagat ctcgcgggtc
    11821 tcagggaagt gcgtgatgta cttcgtgtcg ttgcacggcc acggcacatc cttctgccct
    11881 ggctcaagcg gggcaccgat cgagtgcagc gccttgacga agaagccgtc gtcgccgatc
    11941 ttgtccagcg ctgcctggcc catccgggtc atggtccgca tggacacgac gacatattcg
    12001 gagtcggtga tctcgacacc gagcttggga tcctcggcgc cgagcgggcc catacagaac
    12061 gggacgaccc acatggtccg gccgcgcatg gagccgcggt acaggtcggt catgatgccg
    12121 cgcatctcgg ccggatccat ccagttgttg gtcggccccg cgtcgatctc ccgctcggaa
    12181 cagatgaagg tgcgcgattc cacccgcgcc acgtcggacg gatcggacaa cgcgaggtaa
    12241 gagttgggca gtttctcgtc gttcaaccgc ttgaaggtgc ctgcgtcgac cagatgggca
    12301 gccagtcgct cgaattcctc ttcggagccg tccgcgaatg cgactcgatc cggctgtgtc
    12361 agttcggcga cctcctggac ccaggcaagc agccctttgt gtttagtcgg tgcggtgtcc
    12421 agacctggaa tggtcgctgc ggtcatcaaa actctcctgc gtaggttttc tgccaggacg
    12481 caggccccga tcgcgccaac caaacctgac aggcagcgca ttcggtcgtc cctgtactga
    12541 ggttaacgtg ggtgacatac ccgcggtaaa tcgggagtat gtccgatcac tcacaggaac
    12601 gattcagaaa caatttccgg gccgttttac cgattcagtt cccctcaaat tcgccgcccc
    12661 gctggatagc cgttcgtcta ctccactatt cggtacggcg atctgactac atcgtcgcgc
    12721 cgtacagctc ggcgcgcacc gcctcgatcg ccttctggag cagaggcagg cggcggtccc
    12781 gcaccgtggt ggcgcgtgcc gtcgcaatcg cgtgctcgtg tagctccgcg tcgattttgg
    12841 ccacgcgatc ggcggccgtc gcgctctcga gctcgtcgcg ggtagccagc tccgacgtca
    12901 gcgccgtttc ggccgctagc accctggtcg cgacacgctc ctcgagcgcg gaccgcaggg
    12961 cggccgtgac gtcggtgacc cagcggtcca gcatcgcccg gtcatgaagc agtccgcgga
    13021 tacccaccac ccacaccgtg agaagcagac ccaccaaccc gccggcggcg agccccgcaa
    13081 cggtcaaccc gggcgccagc cccgcgaaca ggcgagtcac catcagtgcc acccccagac
    13141 cgaagcccgc accgaggatc atcatcagct gcgtttccag ccgccgtgat ttcaagggtg
    13201 gcggggaaac ctccggcggt ggcatcggtg acggcggcgg gggcggcgtg gagagctcga
    13261 gctcggtcgc caggtcaccc acttgggtgg tgatgccttc gtccacttcg gcaacgacct
    13321 cgtcaacccg ggtccgtacg tatggctcga actcgccgag ccgccgacgg cccagctcgg
    13381 caacgtcctc ctgcagttcg gcgcgcacgg acgtgcaccg gttgcgggcg aaatacgaca
    13441 actgcagccg tgcgtgctgg acctggctac gaagggtgat cgtgcgttca gtcttggcca
    13501 gccgacggtc gcgctgcgtg gcactacgct gctcctgcaa atcggcgatc cgcgcgttac
    13561 gagtgacgcc tgcggcttcc tcttcgaggc gccgcacggc cgtctgcaac cgggtatccc
    13621 acgcgcgcaa gcgatttcga cgtgccagcc tagcgtcgcc gaggtggccg agccgctccg
    13681 ccagctcgtc gatcttggag tcgcctaggt caggagcggc ggccacccca acccacggga
    13741 cgtcgcggta gcgcgcgtcg tgcgcaatca gcgtctgcct gtcggcggcc agcacattgc
    13801 gccagttgcg atgcacatca atcttcgaca cgactccgat gacgaggtcg gtgttggcgg
    13861 cggcaaggtc aagcagcgca cagtccgatt cggtcagccg cgcgaccgcc gacacgataa
    13921 acacgacggc aatcggcgct tcgacgacgg acagctcacc ggtctcgacg aaggtcacgt
    13981 ccggcagccg ctggcgcagc gccgcgagca ggctgctggt gcccgccaac cacggacccg
    14041 tcaccagcac aacatcgcgg gtcactacac cgggtgagct gacgcccggc tcgatcgctg
    14101 ctaccagggc gtccgcggat gcgattgggt cactcatcta accgacccga gcagacgtag
    14161 tgatcccctc gagatggcgg caccgcagct gcgatgcaac gcgtcgaccg gcccgcggcc
    14221 ataccgccgc cagtgcaccg cgcggcgcag atgcgactcc gcttcattgt ctccgtccac
    14281 cgcgatcccc gccgcctcga ccacgtcgac cgccgcggcc atcaccgcga tgacggtctc
    14341 gtcggccgac aggaactccg tcagcccctc atcctcgaat tgcactgcca gcgcccgcaa
    14401 ttcggtgatt gctgatcgca ctcttcgata ccgaaccggg gcgccgacgg cgtcgagctg
    14461 cgcaacgaca cgatcgatct ggctgagccc tcgcagcagc gtcagcacct tcgcgtcgtc
    14521 agcgccctgg gcgaccgcca tcacgccatg ggctatgccg aatcgatcca acgtgtcgag
    14581 caatcgagcc cgaatcgcct ggggcaacgg gtgatcgctc tgcacgaacg cgtcggtcga
    14641 cgtcagatcg gcgggcacag tcaccatcgt gcgcaatgca gccaccaact catcgtccag
    14701 atcggccttg gccagcagcg cgaccatcgg caccgtcggc accccggtca gcgcctggta
    14761 gtcggcggcg cgtcgatgcg ccagcgccag cggcccgccg gtaccgaacc cggccagatc
    14821 ggccttattg agcacggtca acgtcggccg gtccgcattt gcgaggtgcc cccggtcctc
    14881 gggcttgagc gcctccgcga tgaccagcac gcgcacgtcg gccgacgcag gatcggctgt
    14941 gcccgtcacg cccgcagcgg tcagcgcggc cgcgaccgtg tctcgcccca ctccgtcgcg
    15001 gcccagtaca gcaatgcgta atggagcagc aatacgccga atgatcggtg tcaaccgcgg
    15061 attgcggctc tggtcagcga atcgcgtcag cgcatcgacg aaaatctggt ggccttccat
    15121 taacccgatc cgggtgaaac ccggtcccag ggtgcgtgtc ggcctaaatg ttggcacctt
    15181 cgcgggcaca tcgcgaacgg ttgtccccag taccagtcga aggcaactgt tgggtatcaa
    15241 tctgtaccac gatgcgggta ctcaagcctc gatgagcgac catggacgga tgcaaacgcc
    15301 caggccggat gtcgccgagc cgcatttgca tcctcgcgtc accagtttcc gcacccgccg
    15361 ctccacgctc tccggagggc agcaggccac gtgggagcgg ctgtggccgg agatgggcat
    15421 gcacgcccgc gatgccgacg ggcccgcgcc gcagctggac accgaggcct ggttcggccg
    15481 ctcagcgccg gtggtgctgg agatcggctg cggaagcggc acgtcgacgc ttgccatggc
    15541 acaggcggag ccccacctgg atgtcgtcgc ggtggaggtg taccggcgcg gactggccca
    15601 gctgctctcc gcaatcgacc gcgaaggcgt caccaacatc cgcctggtcc gcggcgacgg
    15661 cgtcgacgtg ctcgagcaca tgtttgagcc ggattccctg accggtgtgc gggtattctt
    15721 ccccgacccg tggccaaagg cacgccacca caagcgcagg ctgctgcagc ccgacaccgt
    15781 ggcgctgatc gccgaccggc tgcgcgccgg cggggtgctg catgccgcca ccgaccactc
    15841 cggctacgcc gagcagatcg cggaggtggg cgacgccgaa ccgcggctac gccgcgtcac
    15901 cgcatcggat tcgctgccga tctcggtgca gcgacccgtc acgaagtacg agggcaaagc
    15961 ccagcacgtg ggcagcgccg tcgccgaatt gctttgggag aaacggccat gagcctcact
    16021 gaagacatcc cacacataga agatgccccg ccgccggtgg caccggcaga ggcgacggcc
    16081 cgagtgctgc tggtgtggga cgcgccgaac ctggacatgg ggcttggctc gatcctcggc
    16141 ggcaggccca cggccgcgca ccggccccga ttcgacgcgc tgggccgctg gctgctgggc
    16201 cgcacggccg cgctgtccgc cgagcgcccc gacgtcacgc tggagcccga agcgaccgtc
    16261 ttcaccaaca tcgccccagg tagcgccgac gtcgttcggc cgtgggtgga ggcattgcgt
    16321 aacgtgggct tcgcggtctt cgcgaaaccg aagatcgacg aagacagcga cgtcgacagc
    16381 gacatgctca accacatcgc gctgcgccgc agcgaggggc tggccggcgt ggtggtggcc
    16441 tccgccgatg gacaggcgtt caaacaaccg ctggaggaaa tcgcccgcga gggggtaccc
    16501 gtccaggtgc ttggatttcg cgaacatgcc agttgggcgt tagcgtcgga taccttggag
    16561 ttcgtcgacc tggaagacat tcctggtgtt ttccgggagc cgctaccgcg aatcggcctt
    16621 gattcgctgc ccgagcaggg ggcttggctg cagccattcc ggccgctgtc ctcgctactg
    16681 acctcgcgcg tatagacaac tggaaatgcg cgggtcgcca cagacttaga aggagcttac
    16741 gtgttcgcct ggtggggtcg aacggtgtac cgataccgat acatagtcat cggtgtcatg
    16801 gtcgcactgt gccttggtgg cggcgtgttc ggcatcagcc tcggcagcca tgtcacgcag
    16861 agcggcttct atgacgacgg tagccagtcg gtgcacgcct cagttatcgc ggacaaggca
    16921 tacggacgcg acacgtcggg ccacatcatc ggggtctaca cggcgcccga gggcaagacg
    16981 gtcgacgacc cggatttcaa gcagaagatc ctcgacaatc tcaagaaggt cgaggaggac
    17041 cacccggacg agatcctgcg ctcgatcggc tacttcaaga gtcccgacgt gctgaagaac
    17101 atgtccgacg cagacaagca gcacgcgttc atgtcgattc agctaaaggg cgacaacgac
    17161 gacgcgatcc tgaacaacta caagaaggtc aaggacgacc tgctcaaggt caaagacgac
    17221 ggcgtcgact tcagaatggc gggtctgcag ccgctggcgg ccgaactgac cggcaccatc
    17281 ggcgacgacc agagacgtgc cgaggtcgcc gccatcccgc tggtgtgcgt ggtgttgttc
    17341 ttcgtgttcg gtggtgtcgt cgcggccgcc ctccccgggg tcatcggtgg cctgaccatc
    17401 gcgggcgcgc tcggcatcat gcgtttggtc gccgagttcg ccccggtgca cttcttcgct
    17461 cagcctgtgg tgaccctcat gggcctcggt atcgccgtcg actacggcct gttcatggtc
    17521 agccgcttcc gtgaggagat cgccgagggc tacgacaccg aggccgcggt ccgaagaacc
    17581 gtgatgacct cgggccgcac gatcatgttc tccgcggtga tcctcgtggc gtcgtcggtg
    17641 ccgctgctgc tgttcccgca gggcttcctc aagtccatca cctacgcgat catcgcctcg
    17701 gtcatgctcg cggcgatcct gtcgattacc gtgctggccg ccgcgctggc catcctgggg
    17761 cccaacgtcg acgcgctcgg tgtgcgcacg ctgctgcggg tgccgttctt ccgtaactgg
    17821 aagccgatgc gcatttacct gacctggctg gccgataaga cgcagaagac caagacgcgt
    17881 gaagaagtcg agaagggctt ctggggcaag ctggtcaacc gcgtgatgaa gcgaccggcc
    17941 gcgttcgcgg tgccgatcat catcgtcctg atcgcgctga tcatcccgtt ggggcagctc
    18001 gcgctcggcg gcatcagcga gaagtacctg ccacccgaca actcggtacg ccaggcgcag
    18061 caggagttcg acaagatctt cccgggcttc cgcaccgagc cgctgacgat cgtgatggaa
    18121 agcgacaacg ggcagcccat caccgaccag caggtcgccg aggtccgcaa caaagcgatg
    18181 cagatctccg gcttcacgga cccggacaac gatccgtcca agatgtggca ggcacgcccg
    18241 tatctcgatg gggcgtcgaa acagccgtcc atccgggtca tccagaacgg cttggtgaac
    18301 cgcaacgacg cagccaagaa gatcgaagag ctgcgcgcca tgacaccgcc gcgtggcatc
    18361 accgtatccg taggcggcac accagctttg gagcaggaca gcatccacag cctgttcgac
    18421 aagctgccgt tgatggtggt cctgctgatc accacgacca cgatcctgat gttcctggca
    18481 ttcgggtcgg tggtgctgcc gatcaaggcc gcggtgatga gcgcgctcac actcggttcg
    18541 acgatgggcg tgctcacctg gatgttcgtc gacgggcacg gttccggcct gatgaactac
    18601 accccacaac cgttgatggc accgatgatc gggttgatca tcgcggtgat ctggggtctg
    18661 tcgaccgact acgaagtgtt cctggtgtcc cgcatggtgg aggcgcgtga gcgcggcatg
    18721 tccaccgccg aggccatcag gatcggtacc gccaccacgg gccggctgat caccggcgcc
    18781 gcgctggtgt tggccgtcgt cgcgggcgcg ttcgtgttct cggacctggt gat
//
LOCUS       DASRJA010000790         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3954947,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000790 DASRJA010000000
VERSION     DASRJA010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3954947"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFC01_35610"
     CDS             complement(<1..387)
                     /locus_tag="VFC01_35610"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZN84985.1"
                     /translation="MITIDPGDLDPTSSYKLLIGSILPRAIAWVSTRSTTGVGNVAPV
                     SFFTVVGRFPPVLSITLQPRSDGVTLKDTFVNIRDTDEFVVNIVSIGLADAVHRSAYE
                     FDSDVDEFEALGLKKAASEVISAPRIG"
     gene            complement(403..1689)
                     /locus_tag="VFC01_35615"
     CDS             complement(403..1689)
                     /locus_tag="VFC01_35615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZN84986.1"
                     /translation="MSELHYEVLVNDGLRRHREQRLPDGSPIISSPVASTLIYGDNDA
                     VLVDPPFTYEQVKRVGDWIEASGKHLTAVYATHGHGDHWFGTELLLQRFPYAVAYATT
                     GTISMMHQQGTVGRAELWDVDFPGQIPPSPVNYRTIPADGIELEGHRLLAVEVGHTDT
                     DDTTVLHVPSIGLVVAGDVAYNGVHQYLLESAHGGVDAWLVALDKVEALQPRAVVAGH
                     KNKDLPDNPAIIDQTRDYLLNARRLRAESGSPRAYFDAMVALYPDRLNVGPVWYTAVA
                     LLGGRTVHDEVAHWFFDDYLTTWIGVGAGMIARGPEFILDYWSAPLNWSDDQSSRWLL
                     DTPSVVGLLDALQTRLRAEGYADTAVPDRRVTVYHDNGAAIEVIWSRLRADGTEIERL
                     AAHFEVARGPEGWRIISIQATPTTSDSLNAVWPQAN"
     gene            complement(1709..2611)
                     /locus_tag="VFC01_35620"
     CDS             complement(1709..2611)
                     /locus_tag="VFC01_35620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZN84987.1"
                     /translation="MHRQDVEFTVEGDVILRGWLFTPDGPGPHPAITMAHGFAGVKEH
                     GLERFARAFADAGFVVLVHDHRGFGASDGSPRNDIDPWVQIADWRRAISFLESQPFVD
                     PDRIGLWGSSYAGGHAIVLGATDRRLRAVVAQVPTISGYKQGLRRVAPDQVPALEAAF
                     ADDERQQFRGAPPATQAVVSADPSVPAAYRSPEAIAFYTQPVPEGVWKNTMTLRSTRA
                     ARMYEPGTWITRVSPTPLLMVVGLHDTITVTDLALAAYEKALQPKKLVTIDGDHFDAY
                     EKRFDEASGAAIAWFTEHLKSENN"
     gene            2729..>2906
                     /locus_tag="VFC01_35625"
     CDS             2729..>2906
                     /locus_tag="VFC01_35625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZN84988.1"
                     /translation="MAARLNALGDYLRARREQLRPEDVGLVAGSRRRVAGLRREELAL
                     LAGISADYYLRLEQG"
BASE COUNT          492 a          882 c          991 g          541 t
ORIGIN      
        1 gccgatgcgg ggtgccgaga tcacctcgga cgcggccttc ttcaggccga gggcctcgaa
       61 ctcgtctacg tcgctatcga attcgtaggc agagcggtgc acggcatctg ccaggccgat
      121 cgagacgatg ttgacgacga actcgtcggt gtcgcggatg ttgacgaacg tgtctttgag
      181 agtcaccccg tcggagcgcg gctgcaacgt gatcgacaaa accggcggaa aacgtccgac
      241 gacggtgaaa aaggataccg gcgcgacatt gccaaccccg gtggtcgacc gggtgctgac
      301 ccaggcgatt gcacgcggca atatgctgcc gatgagaagt ttgtagctcg aggtcgggtc
      361 gagatccccg gggtcgatgg tgatcatcat tgtctccttt gttcaattcg cctgcggcca
      421 tacggcgttc aacgagtcgc tagttgttgg cgtggcctgg atgctgatga ttcgccagcc
      481 ttcaggtcct cgtgctacct cgaagtgagc ggccaaccgc tcgatctcag tgccgtcggc
      541 acggaggcgt gaccagatca cctcgatcgc ggcaccgttg tcgtgataga ccgtcactct
      601 gcggtcgggc acggcggtgt ctgcgtaacc ctcggcgcgt aaccgggtct gcaatgcgtc
      661 cagcaatccc accaccgacg gcgtgtccag aagccatcgg ctgctctggt cgtcgctcca
      721 attcaggggt gcagaccagt aatcgaggat gaactcggga ccgcgcgcga tcatgcctgc
      781 gccgacaccg atccaggtgg tcaggtagtc gtcgaagaac cagtgcgcga cttcgtcatg
      841 aaccgttcgg cccccaagca gcgccaccgc ggtgtaccag acggggccta cgttgaggcg
      901 gtcggggtac agggcgacca tcgcgtcgaa atacgctctc gggcttccac tttccgcgcg
      961 aagccgacgg gcgttcagca gatagtcgcg ggtctggtct atgatggccg ggttgtcggg
     1021 aaggtccttg ttcttgtgac cggcgacgac ggcgcgtggc tgcagcgcct cgaccttgtc
     1081 caacgccacc agccaggcat cgactccgcc gtgcgcgctt tccagcaggt attggtgcac
     1141 gccgttgtag gcgacgtcgc cggcgacgac caagccgatg gagggcacat gcaacacggt
     1201 ggtgtcgtcg gtatcggtgt ggccgacctc gactgcgagc agacggtggc cttccagctc
     1261 gattccgtcg gccggaatgg ttcgatagtt gaccggactg gggggaatct ggccggggaa
     1321 gtcgacatcc cacagttcgg cacggccgac ggtgccctgc tggtgcatca tcgagatcgt
     1381 gccggtggtc gcataggcca cggcgtaggg aaatcgttgc agcagaagtt cggtgccgaa
     1441 ccagtggtcg ccatgcccgt gggtggcgta cacagcggtc aggtgcttgc cagatgcttc
     1501 gatccagtcg ccgacgcgct tcacctgctc gtaggtgaac ggtggatcga ccaggacggc
     1561 gtcgttgtcg ccgtagatca gcgtggaggc gaccggcgac gagatgatcg ggctgccgtc
     1621 gggcagccgc tgctcgcggt gacgtcgcag tccgtcgttt accagaactt cgtagtgcaa
     1681 ttcagacatg tcgtaattcc cttcgggatc agttgttttc ggacttgaga tgttcggtga
     1741 accatgcgat ggccgcaccg ctggcttcgt cgaagcgctt ctcgtacgcg tcgaagtgat
     1801 cgccgtcgat ggtgacgagc ttctttggtt gcaaagcctt ttcataagca gccagagcga
     1861 ggtcggtgac ggtgatggtg tcgtgcaggc cgacgaccat caataggggt gtgggggaga
     1921 cgcgcgtgat ccacgtaccc ggctcgtaca tgcgggcggc gcgggtcgac cgaagtgtca
     1981 tggtgttttt ccacacgccc tcgggcaccg gttgggtgta gaacgcgatc gcctccggtg
     2041 accggtaggc cgcaggcacc gacgggtcgg cgctgaccac agcctgggtg gccggtggtg
     2101 cgccgcggaa ctgttggcgt tcgtcgtcgg cgaacgccgc ctccaaggcc ggcacctggt
     2161 cgggcgcgac gcgtcgcagg ccctgcttgt agccgctgat ggtgggcacc tgcgcgacga
     2221 cggcccgcag ccgccggtcg gtggccccca gcacgatcgc gtgcccgccc gcgtagctgc
     2281 tgccccacag gccgatccgg tcggggtcga cgaacggttg gctctccagg aaggagatcg
     2341 cccggcgcca atcagcgatc tgcacccacg ggtcgatgtc gttgcgcggc gagccgtcgc
     2401 tggccccgaa gccgcggtgg tcgtgcacca gaaccacgaa tcccgcgtcg gcgaaagccc
     2461 gggcgaatcg ctccagcccg tgttccttca cgccggcaaa gccgtgcgcc atcgtgatgg
     2521 ccggatgcgg accggggccg tcgggcgtga acaaccagcc ccgcagaatc acatcacctt
     2581 cgacggtgaa ttcgacgtcc tgtcgatgca ctttccgctc cttccacgtg tcttcaagcg
     2641 gcacaacatc cacgatgtgc ccacttcgtt cgaggagggg agaccgcaat gaccctggta
     2701 ttgggaggga caggattacg ctcggttggt ggcggcgagg ctcaacgcgc tgggcgacta
     2761 cctgcgggcc aggcgtgagc agctgcggcc cgaagacgtc gggttggtgg ccggctcacg
     2821 gcggagggta gcgggcttgc gccgtgagga gctggcactg ctggccggga tcagtgccga
     2881 ttactacctg cgactcgagc agggcc
//
LOCUS       DASRJA010000791         2798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3996930,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000791 DASRJA010000000
VERSION     DASRJA010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3996930"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VFC01_35630"
     CDS             <1..823
                     /locus_tag="VFC01_35630"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN84989.1"
                     /translation="EEAVLLRTRMTRERVLESLLRDLLTYDADVPADLPTRARDFGFD
                     LGLPRQAIVIEVLVPAGLGSPSSSPLRLVREVFHSPQDLSCGLSSSRYVVLRSQHNGR
                     PRLGRDDLLDLTGRIHDLVGATCRMGVGGVAPDERGIATSYSEALMALRVGSSNGQPS
                     PHDIADLRAEQLILSVPPAARARITTETLGDLRSLGDWDSTRATVIAWVESGFVLVDA
                     AAALTIHRNTLVYRLGRISKHTGWPTSDRRHWLALYLACLADALGAESAVRSRSG"
     gene            complement(1186..1926)
                     /locus_tag="VFC01_35635"
     CDS             complement(1186..1926)
                     /locus_tag="VFC01_35635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZN84990.1"
                     /translation="MTQLDGKVALVTGASAGLGAATAQLFAERGASVFGIARDADRMA
                     TVFGEVPGGKYSSVDISDPAACKQAVEDCVAEFGRLDILANIAGFHQMRHTPTMTDDD
                     WDRDLAVNLNGPFYLCRAALPHLLESGGNIVNVSSIAGVEGEVYSAGYCAAKHGLIGL
                     TRALAVEFTKEKLRVNAVCPGGMPTAQSTEFQAPDNADWNLILRIASPRGFMETVDVA
                     KAIAFLASDDAAAIHGAVYRVDNGKGAG"
     gene            complement(1957..>2798)
                     /locus_tag="VFC01_35640"
     CDS             complement(1957..>2798)
                     /locus_tag="VFC01_35640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZN84991.1"
                     /translation="PLVGRSEPAPAPKDASVLLRVDELDVRFGVTTPVGRSTVHAVKR
                     LWFEVRRGTTLGLVGESGSGKSTVAGALTGLVEPDVGTAQLDGVEIFGVRGAAAKELR
                     KKIGLVFQDPYSSLNPRARVQTAIDEPLRVHRLANGKPARRARAAELLELVGLPPSFA
                     SRYPHELSGGQRQRVSIARALAAEPELLILDEATASLDVSVQAHVLELLADLQRELGL
                     TYLFIGHDLAVIQQMSHDVLVMRDGEAVEYRPAAELFAEPEHEYTRTLLAAVPPARPR
                     AAV"
BASE COUNT          449 a          988 c          906 g          455 t
ORIGIN      
        1 cgaggaagcc gtgctgctgc gcacccggat gacccgcgag agggtgctgg agtccctgct
       61 gcgcgacctg ctgacctacg acgccgacgt ccccgccgac ctgccgaccc gcgcccgcga
      121 cttcggcttc gacctgggcc tgcccaggca ggcaatcgtg atcgaagtct tggtgccggc
      181 gggtctgggc agtccgagct cctcgccgct gcggcttgtc cgcgaggtct tccacagtcc
      241 gcaggacttg tcctgcggac tgtcgtcctc gaggtacgtc gtgctgcgaa gccagcacaa
      301 cgggcgaccg cggctggggc gagacgatct cctcgatctg acggggcgca tccacgacct
      361 cgtcggcgca acctgtcgga tgggagtggg cggagtcgcc ccggacgagc gtgggatcgc
      421 gacgtcctac tccgaggcgc tgatggcgct tcgcgtcggc tcctccaacg gacagccctc
      481 gccccacgac atcgccgacc tccgggcaga gcagctcatc ttgtctgtgc cccccgctgc
      541 ccgggcccgg atcaccaccg agaccctggg cgacctgagg tccttgggcg actgggactc
      601 gacccgggcc acggtgatcg cttgggtgga gagcgggttc gtcctggtgg acgccgcggc
      661 cgcactcacc atccaccgca acaccctcgt ctaccgcctc gggcggatct cgaagcacac
      721 cggctggccc acatccgacc gccggcactg gctcgcgctc tacctcgcct gcctggcgga
      781 cgcgctgggc gctgagtcgg cagtccggag ccgttcgggc tgacgccggc gccgctggcc
      841 gtgccctacc agtacgccac ggtcagaacg aatctggctt tttgctaggg acacaggcga
      901 tttcgccggc gcgatccaca acacgtgcga cggcgccggc gacccgcagc aattcgcgct
      961 cgacaccgac ggtgtgtcgg gactcggggc gacgttccgc ctcggtacgt cggccacccg
     1021 attcgacacc gacggttcgt cgatcacccg aatcatcacc tccgacgggc caattcacgc
     1081 tgaccgcgca agcgacaccg ctggccggca cctcgaaact gcggggagat cgcgaatttt
     1141 cacgagatcg cgatctgtcc gcagtttcgc gagtggtggc tgacgtcagc ccgcgccttt
     1201 gccgttgtcg acgcggtaga ccgcaccatg aatcgccgcg gcgtcatcgc tcgccaggaa
     1261 cgcgatcgcc ttggccacgt cgacggtctc catgaatccg cgtggcgatg cgatgcgcag
     1321 gatcagattc cagtcggcgt tgtcgggtgc ctgaaactct gtcgattgcg cggtcggcat
     1381 gccgcccggg cacaccgcgt tgacgcgcag cttctccttg gtgaactcga cggccagcgc
     1441 gcgggtcagc ccgatcagcc cgtgcttggc cgcgcagtag ccggccgagt acacctcacc
     1501 ctcgacgccg gcaatcgagg agacattgac gatgttgccg ccggattcca acaggtgcgg
     1561 aagcgccgcg cgacacaggt agaacggccc gttgaggttg accgcgaggt cgcgatccca
     1621 atcgtcgtcg gtcatcgtcg gcgtgtgccg catctgatgg aaccccgcga tattggccag
     1681 gatgtcgagg cggccgaatt ccgcgacgca gtcttcgacg gcctgcttgc aggcggcggg
     1741 atcggagatg tccaccgatg agtacttgcc gcccggcacc tccccgaaca ccgtcgccat
     1801 ccggtcggcg tcgcgtgcga tgccgaacac cgaagcaccg cgttcggcga agagctgtgc
     1861 cgtcgcggcg ccgagtcccg ccgaggcgcc ggtgaccaac gcgaccttgc catccagttg
     1921 tgtcatggca agaccttctc acgaggcctc gcggggttag acggcggcgc gcggtcgcgc
     1981 tggcgggact gccgccagca gtgtgcgggt gtactcgtgc tcgggttcgg cgaacaactc
     2041 cgcggcgggc cgatactcga ccgcctcgcc gtcgcgcatg accaagacgt cgtggctcat
     2101 ctgctggatg accgccaaat cgtggccgat gaacagatac gtcagcccca gctcgcgctg
     2161 caagtctgcg agcaattcga ggacatgggc ttgcaccgag acatccagcg acgcagtcgc
     2221 ttcgtcgagg atcagcagct cgggctcggc ggcaagcgcg cgggcgatgc tcacccgctg
     2281 tcgctgccca ccggacagtt catgcgggta gcgggaagcg aacgatggtg gcaggccgac
     2341 gagttcgagc agctccgccg cccgggcccg gcgggcgggc tttccattgg caagtcgatg
     2401 gactcgtaat ggttcatcga tcgccgtctg cactcgggcg cgtgggttca gcgacgagta
     2461 cggatcctga aacaccaagc cgatcttctt ccgcagctcc ttggctgcgg caccccgtac
     2521 gccgaagatc tcgacaccgt ccaattgtgc ggtgcccaca tcgggttcga cgagcccggt
     2581 caacgccccg gcgaccgtcg acttgcccga acccgactcg ccgaccaaac ccaatgtcgt
     2641 gcctcgccga acctcgaacc aaagccgctt cacggcatgc accgtcgacc tgccgaccgg
     2701 cgtggtcact ccgaaccgca cgtcgagctc gtcgacgcgc agcagcaccg atgcgtcttt
     2761 cggcgccggc gcaggctccg accgcccaac gagcggcc
//
LOCUS       DASRJA010000792         4831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3514278,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000792 DASRJA010000000
VERSION     DASRJA010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3514278"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFC01_35645"
     CDS             <1..645
                     /locus_tag="VFC01_35645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HZN84992.1"
                     /translation="EQLGSLLTDGERVSAHLVMTAERVVAAEAVRTLGSLALMGVQVN
                     ELIVNQILVQDDSYEYRNLPEHPAFDWYSERISEQRAVLDELDSTIGGVQLVLVPHLA
                     GEPIGPKALGELLDSARRRDGSPPPGPLRPIVDRESGTGLESVYRLRLELPQVDSAAL
                     TLGRVDDDLIIGAGAMRRRVRLASVLRRCIVMDAQLRGSELTVRFRPNPEVWPA"
     gene            642..1040
                     /locus_tag="VFC01_35650"
     CDS             642..1040
                     /locus_tag="VFC01_35650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84993.1"
                     /translation="MTGSHPDIGPELRQLAQTILDKLDPAVRLAAARAAGTGEGPGKC
                     QQVWCPVCALAALATGEQHPLLDVIAEHSVALLAVIRAVIDGSAGTAAGPSGTEPPPD
                     SPPPPTGPGPDEPPPTGPGRYQHIPVTVEE"
     gene            1124..1849
                     /locus_tag="VFC01_35655"
     CDS             1124..1849
                     /locus_tag="VFC01_35655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZN84994.1"
                     /translation="MWYWLFKYIFMGPLLSLLGRPKVEGLDYVPHSGPMILASNHLAV
                     ADSFYLPLVVSRRITFLAKAEYFTGTGLKGWFQRWFYTAAGQVPIDRTDADSAQSALN
                     TAERILNEGKLLGMYPEGTRSPDGRLYKGKTGLARLALESGVPVIPVAMIGTNVVNPP
                     GSKMWRFGRVQVKFGKPMDFSRFEGLAGNRFIERAVIDEVMYELMRLSGQEYVDLYAA
                     DVKEGKGATAIKPPARMPEAAAG"
     gene            complement(1809..3086)
                     /locus_tag="VFC01_35660"
     CDS             complement(1809..3086)
                     /locus_tag="VFC01_35660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HZN84995.1"
                     /translation="MSIWRSPGGGGLGPTLAWRLFQLLTLAALAWAGWQLLGHIPYRI
                     DIDVYRMGGQAWLDGRPLYADGAMFHTRGGLDLPFTYPPLAAVAFAPFAWLSLNVASV
                     AITLTTLVLLIASMVIVLTGLNVWPQATVTSEPAWLRRGWLAAAIVAPAVIYLEPIRS
                     NFDFGQINVVLMTLVIADCVPRKTPWPRGMLLGVAIALKLTPAVFLLYFLLRRDTRAL
                     IVTAASAAVATLAGFAFAWRDSWEYWTETVRNTDRIGTVTLNTNQNIAGALARLGLGE
                     GERFVVWTLACFAVLALTVWAARRVLNAGEPVLALICVAMFGLVVSPVSWSHHWVWVL
                     PTLVVSAVVAYRRRHVALGLVTLAGLALMVWTPIPLMPEHHETSASLWRQLAGGSYLW
                     WAIAMIAVAGTVSARSADRSVSRPLLQASAPAA"
     gene            3171..4055
                     /locus_tag="VFC01_35665"
     CDS             3171..4055
                     /locus_tag="VFC01_35665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN84996.1"
                     /translation="MHATFDFSLSIPHGRIPLVFCLVAFILTFFMTRTIVRYIRSHAN
                     SDAPRKWWQPRNISAGGGLHIHHVVIGVILVMVSGISMVTLAVNGGVPEFTVAATFFG
                     IGAALVLDEFALILHLQDVYWAEDGRTSVDAVFAAVAVAGLLILGFNPLSFFDVNIWR
                     EDSSFWPRALVVAGAVLTLLVAVVVLLKGKVWTGLVGMFMTPLLFIGAIRLSRPHAPW
                     ARWRYGTKPRKMHRALERERRLRRPVVQAKLWLQHVIAGEPHFPDDSEVDQQLDREIH
                     AAPAPPDRQTVNRETAEV"
     gene            4052..4561
                     /locus_tag="VFC01_35670"
     CDS             4052..4561
                     /locus_tag="VFC01_35670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyadenylate-specific 3'-exoribonuclease AS"
                     /protein_id="HZN84997.1"
                     /translation="MRYFYDTEFIDNGRTIELISIGVAAEDGREYYAISTEFNPDRAG
                     SWVRKHVLPKLPSPSSPLWRSRRQIRTELEDFFGVDGDEPIELWAWVGAYDHVVLCQL
                     WGPMTDLPPAIPRFTRELRQFWEDHGSPRMPPRPRDAHDALVDARHNLLRYRLMTTGF
                     EKGVVPAQR"
     gene            4595..>4831
                     /locus_tag="VFC01_35675"
     CDS             4595..>4831
                     /locus_tag="VFC01_35675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HZN84998.1"
                     /translation="MNWTVDVPIDQLPPLPPLPDDLRHRLDAALAKPAAQQPSWDAES
                     AKVMRTVLESVPPVTVPAEIERLRGLLADVARGEA"
BASE COUNT          852 a         1573 c         1624 g          782 t
ORIGIN      
        1 gagcagctgg gctcgctgct caccgacggc gaacgggtca gcgcacacct cgtgatgacg
       61 gcagagcggg tggtcgccgc agaggcggtc cgcacgctgg ggtcactggc cttgatgggt
      121 gtgcaggtca atgaactgat cgtcaatcag attctggttc aagacgattc gtatgagtac
      181 cgcaatcttc ccgagcaccc cgcgttcgac tggtattccg agcgcatctc cgagcaacgg
      241 gcggtgctcg acgagctgga cagcacgatc ggaggtgtgc aactcgtgct cgtcccgcat
      301 ctggcgggcg aaccgatcgg ccccaaggcg ctcggcgaac tgctcgacag cgcgcggcgg
      361 cgcgacggat caccgccacc cgggccactg cgaccgatcg tcgaccggga atccggaact
      421 ggactcgaat cggtgtaccg gttgcggcta gagttgccgc aggtcgactc cgccgccttg
      481 acgttgggtc gggtcgacga cgacttgatc atcggcgcag gcgctatgcg gcgccgggtt
      541 cgactggcat ccgtcctgcg gcgctgcatc gtgatggatg cgcagctacg aggtagcgaa
      601 ttgaccgtgc gttttcgacc gaatccggag gtgtggccag catgaccggg tcccaccctg
      661 atatcggtcc cgaactgcga cagctggccc agaccattct cgacaagctc gatcccgcgg
      721 tgcgactggc tgcggctcgc gccgcgggca ccggcgaagg accaggcaag tgccagcagg
      781 tgtggtgccc ggtgtgtgca ctggcggcgc tggcgaccgg ggagcagcat ccgctgctcg
      841 atgtcatcgc cgagcacagc gtcgcgctgc tggcggtgat ccgcgccgtc atcgatggct
      901 cggcaggcac agcggcgggc ccgtccggca ctgagccgcc accggacagt ccgccgccgc
      961 cgaccggacc gggcccagat gaaccaccgc cgactggccc gggtcgctat cagcacatcc
     1021 cggtgacggt cgaggagtaa gtgttgcgcg tgtggtcgca tccacaccgc gtaggtaaag
     1081 ttgtcgcaaa gcaccgtccg gtgttcggtg atggggaggg tccatgtggt actggctttt
     1141 caagtacatc ttcatgggtc ctttgctgtc tctgctcggc cgtccgaaag tcgaagggct
     1201 ggactacgtt ccgcactccg gtccgatgat cttggccagc aatcaccttg ccgtcgctga
     1261 cagcttctac ttgcccctcg tcgtgagtcg ccgaatcacg ttcctggcca aggccgaata
     1321 cttcaccggg acggggctca agggctggtt tcagcgctgg ttctacacgg ccgccgggca
     1381 ggtgccgatc gaccgcacgg acgccgacag cgcccagagc gcgctcaaca cagccgagcg
     1441 gatccttaac gaaggaaagt tgctcggcat gtaccccgag ggcacccgtt cacccgacgg
     1501 gcggctgtac aagggcaaga ccggtctcgc acgcctggcg ctggagagcg gagtgccggt
     1561 cattccggtc gcgatgatcg gaaccaacgt ggtcaatccg ccaggcagca agatgtggcg
     1621 gttcggtcgg gttcaggtca agttcggcaa gccgatggac ttcagccggt tcgagggcct
     1681 ggccggtaat cggttcatcg agcgcgccgt catcgacgag gtgatgtacg agctgatgcg
     1741 gctgtccggt caggagtacg tcgacctcta cgccgccgac gtcaaagagg gaaaaggcgc
     1801 gacggcgatc aagccgccgg cgcggatgcc tgaagcagcg gccggctgac cgaccgatcg
     1861 gctgatcgcg cggacaccgt ccccgcgacc gcgatcatcg cgatcgccca ccacaagtac
     1921 gaaccacccg cgagctgacg ccacagcgac gccgaggttt cgtgatgctc gggcatcagc
     1981 ggaatcggcg tccacaccat caatgcgagg ccggccaacg tgaccagccc gagcgccacg
     2041 tgccggcgcc gataggccac caccgcagac acgaccagcg ttggcaagac ccacacccag
     2101 tggtgcgacc acgacaccgg tgacaccacc aggccgaaca tcgccacgca gatcaacgcc
     2161 agcaccggct cgcccgcgtt gagaacccgt cgcgccgccc acaccgtcaa cgccagcacc
     2221 gcgaagcagg ccagtgtcca cacgacgaac cgctcgccct cgccgagacc gaggcgggcc
     2281 agcgcgcccg cgatgttctg gttggtgttc agggtcaccg tgccgatccg gtcggtgttg
     2341 cgcacggtct cggtccagta ctcccaggag tcccgccacg cgaacgcaaa accggccagt
     2401 gtggccacgg ccgccgatgc tgctgtcacg atcagcgcgc gggtgtcgcg gcgcagcagg
     2461 aagtacagga ggaacaccgc aggggtgagc ttgagggcga tcgcgacccc aagcagcatg
     2521 ccgcgcggcc acggcgtctt gcggggcacg cagtcggcga tgacaagggt catcagcacg
     2581 acgttgatct gaccgaagtc gaaattcgac cggatgggtt cgaggtagat gacggcaggc
     2641 gccacgatcg cggccgccag ccagcctcgc cgcagccacg ccggctctga ggtgacggtg
     2701 gcttgcggcc acacgttcag cccggtcagc acgatcacca tcgaggcgat cagcagcacc
     2761 agagtcgtca gcgtgatcgc gacgcttgcc acgttgagcg acagccacgc gaacggcgcg
     2821 aacgcgacgg ccgccagcgg tggataggtg aagggaaggt caagcccacc gcgggtgtgg
     2881 aacatcgccc cgtcggcgta cagcggccgg ccgtcgagcc aggcctgccc gcccatccga
     2941 tagacgtcga tgtcgatgcg gtacggaatg tggcccaaga gctgccaacc agcccaggcc
     3001 aacgccgcca gggtgagcag ctgaaacagc cgccaggcga gcgttggccc caaaccaccc
     3061 ccgccgggcg accgccaaat actcatgtcg ccgaccagcc tatcggtgtc ctccggcgag
     3121 tcggcgggta tccatcccat gacacgcgca ggtcggcgta agttttctgc gtgcacgcga
     3181 cgttcgactt cagcctgtcc attccgcacg ggcggatacc gctcgttttc tgcctcgtcg
     3241 cgttcatcct cacgttcttc atgacccgca cgatcgtgcg gtacatccgc agccacgcga
     3301 acagcgacgc accgcgcaag tggtggcagc cgcgcaacat ttcggcgggc ggcggcctgc
     3361 acatccacca cgtcgtgatc ggtgtgatcc ttgtcatggt ctccgggatc tcgatggtga
     3421 cgttggcagt caacggggga gtacccgaat tcacggttgc ggcaaccttt ttcgggatcg
     3481 gcgcggcgct ggtactcgac gagttcgctt tgatactgca tctgcaggat gtgtactggg
     3541 ccgaggacgg ccgcacgtcg gtggacgccg tcttcgctgc ggtcgcggtg gcgggactgc
     3601 tgatcttggg gttcaacccg ttgtcgttct tcgacgtcaa catctggcgt gaggactcgt
     3661 cgttctggcc acgggcactg gtcgtcgccg gagcggtgct gacgctgctc gttgcggtag
     3721 tcgtgctgct caaaggcaag gtgtggaccg ggctggtcgg gatgttcatg acgccgctgc
     3781 tgttcatcgg cgccattcgg ttgtcgcgac cgcacgcacc gtgggcgcgc tggcggtatg
     3841 gcaccaagcc caggaagatg cacagggcgc tggaacggga acgccggctg cgtaggcctg
     3901 ttgtccaggc gaagctgtgg ttgcagcacg tgatcgccgg ggagccgcac tttccggacg
     3961 acagcgaagt cgatcagcag ctcgaccgcg aaatccacgc ggccccagcc cccccggacc
     4021 ggcagacggt caaccgggaa acggccgaag tgtgaggtac ttctacgaca ccgaattcat
     4081 cgacaatggc cgcaccatcg agctgatctc gatcggggtg gcagccgagg acggccgcga
     4141 atactacgcg atctcaacgg agttcaatcc ggaccgggcc ggtagctggg tgcgcaagca
     4201 cgtgctgccc aagctgccgt cgccgtcgtc gccgctgtgg cgctcgcgca ggcagatccg
     4261 gacggagctg gaggacttct tcggcgtcga cggcgacgag ccgattgagt tgtgggcctg
     4321 ggtcggtgcc tatgaccacg tggtgctgtg tcagttgtgg gggccgatga ccgacctgcc
     4381 gccggcgatc ccgcgcttca cccgcgagct gcggcagttc tgggaggacc atgggtcgcc
     4441 gcggatgccg ccgcgaccgc gtgacgcaca cgacgccctg gtcgacgccc gtcacaacct
     4501 gctgcgttac cgattgatga ccaccggctt cgagaagggc gtagtgccag cccagcggta
     4561 aaaagcaggt gcgggagccc gttaccatga acgggtgaac tggaccgttg acgtacccat
     4621 cgatcagctg ccgccgctgc cgccgctgcc ggatgacctg cggcatcggc tggacgccgc
     4681 gctggccaag cctgcggcac agcagcccag ctgggacgcc gaatcggcca aggtgatgcg
     4741 gacggtgctg gagagtgttc cgccggtgac ggtgcccgcg gagatcgagc gtctgcgagg
     4801 ccttctggcc gatgtcgccc gcggcgaggc g
//
LOCUS       DASRJA010000793        19585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1698616,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000793 DASRJA010000000
VERSION     DASRJA010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 19585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19585
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698616"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFC01_35680"
     CDS             complement(<1..881)
                     /locus_tag="VFC01_35680"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZN84999.1"
                     /translation="MQQLEGGKVVSFASQIDDATIEQAKQTAELPFVFPHVALMPDAH
                     LGKGAAVGSVIPTLGAVIPAAVGVDIGCGMIAARTKFTADDLAGKDTARLRNSLESAI
                     PLSAGNYNQSLRRFAFTQARIKHLEQLAADGHVDLSHSPKWKEQLGTLGGGNHFIELC
                     LDAGDAVWMFLHSGSRGVGHKIATRHIAVAKQQCRDMGVALPNADLAYLSEGTAEFDN
                     YIAELRWAQQFALANREEMMDRFRQSFAHWMGADPEGVELERVNTHHNYSAPERHADK
                     DVWLTRKGAVDAHRGVMG"
     gene            complement(948..3317)
                     /locus_tag="VFC01_35685"
     CDS             complement(948..3317)
                     /locus_tag="VFC01_35685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HZN85000.1"
                     /translation="MITEQTFPVEPWQVRETKLDLNLLAQAESVFALSNGHIGLRGNL
                     DEGEPYGLPGTYLNSFYETRPLPYAEAGYSYPEDGQSVIDVTNGKILRLLVDDEPFDV
                     RYGHLESHERVLDLRAGTLTRHACWTSPAGKRVRLRTTRLVSLAQRSVAAIEYVVEAV
                     DEFVRLTVQSELVANEDQPRPSADPRVAALLLNPLEAVQHDAGERGALLVHRTRSSRL
                     MMAAGMDHQIDVPGRVEVNTDAGEDWARTTIICGLRAGQQLRIVKFLGYGWSSLRSRP
                     ALRDQVLGAVAGAQYCGWEGLLESQRDFLDEFWDCADVEVEGDADCQQAVRFGLFHVL
                     QASARAERRAIPGKGLTGTGYDGHTFWDTEGFVLPVLTYTAPRAAADALRWRASTLDL
                     ARERAAELDLAGAAFPWRTIRGQECSAYWPAGTAAWHINADIAMAFERYRLVTGDDSL
                     EEECGLRVLVETARLWMSLGHHDRHGVWHLDGVTGPDEYTAIVRDNVFTNLMAAHNLR
                     AAVDACGRHPDAAYAMGVGSEETAAWRDAAAAVHIPYDEELGVHEQCEGFTTLREWNF
                     TENTSYPLLLYEPYVRLYPSQVVKQADLVLAMHWLSHEFTPEQKANNVDYYEKRTTRD
                     SSLSACTQAVMCAEVGHLELAHDYLYEAALIDLRDLHHNTRDGLHMASLAGAWTALVA
                     GFGGLRDDDGELALDPQLPDGISCLRFRLRWRNFRLTVDVRHDEVAYTLRDGPESALT
                     IRHAGESLELHTGSPTKVRLRRRKPLSPPPSQPPGREPIRRPSLDRAFG"
     gene            complement(3314..4057)
                     /locus_tag="VFC01_35690"
     CDS             complement(3314..4057)
                     /locus_tag="VFC01_35690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HZN85001.1"
                     /translation="MLGLPDAIRACLFDLDGVLTDTASVHKKAWKAMFDAYLRKSAER
                     SGEQFVPFDIEADYLTYIDGKKRDDGVRSFLDARGITLPDGDPDDDSSAETVYGLGNH
                     KNDLFHETLRKDGVKVFEGSRRYLEATSAAGLAVAVVSSSSNTREVLEITGLDKFVQQ
                     RVDGVTLREEHIAGKPAPDSFLRAAELLDVKPAEGAVFEDALAGVEAGHNGGFGVVVG
                     VDRVGQAEALRRKGADIVVADLAELLPNR"
     gene            complement(4064..4489)
                     /locus_tag="VFC01_35695"
     CDS             complement(4064..4489)
                     /locus_tag="VFC01_35695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85002.1"
                     /translation="MQHSNSRAFALVGVVVAAIAAAALIMPAARQLGPATGLLTAAAI
                     IGIPVLVVLAVSGAPRYGVLASSVVAVVVAVLTCVVSLVVAAFAFATALSGSVTGVVL
                     AIVLFGAPALCVLILGLLALRLVPARSEDVATHRQAQHQ"
     gene            complement(4543..6087)
                     /locus_tag="VFC01_35700"
     CDS             complement(4543..6087)
                     /locus_tag="VFC01_35700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN85003.1"
                     /translation="MTTVQTSKLPTVEDLRNQARDALTAVGSRTELDAPGGHGLQAST
                     PISGEVLFTVAETTPEQTDAAIAEAAQAFTAWRTTPAPVRGALVARLGELLVEHKADL
                     ATLVTIEAGKITSEALGEVQEMIDICEFAVGLSRQLYGKTIASERPGHRLMETWHPLG
                     VVGVITAFNFPVAVWSWNTAIALVCGDTVVWKPSELTPLTALACQALIERAAIDVGAS
                     EHIARLVQGGREVGERLVDDARVALLSATGSVRMGQQVGPRVAQRFGKALLELGGNNA
                     AIVTPSADLDLAVRGIVFSAAGTAGQRCTSLRRLIVHSSVADELVDRIVNAYRQLPIG
                     DPASDGTLVGPLIHETAYRDMVRALEQARADGGEVFGGERHDLGDESAYYVAPAVVRM
                     PSQTAVVHAETFAPILYVLTYDDLDEAIGLNNAVPQGLSSAIFTLDVREAERFMAADG
                     SDCGIANVNIGTSGAEIGGAFGGEKQTGGGRESGSDAWKAYMRRATNTVNYSSELPLA
                     QGVHFG"
     gene            complement(6084..7286)
                     /locus_tag="VFC01_35705"
     CDS             complement(6084..7286)
                     /locus_tag="VFC01_35705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN85004.1"
                     /translation="MSRRIETWQLRAKFAAALSHLYGAEVPAYNTLVEVSAEVNRELG
                     ASERVTAERHGAIRVGSARELADVADLFAAFGMSPVGFYDLRDAASPVPVVSTAFRPI
                     DTEELGHNPFRVFTSMLATADARFFSPDLCGRVERFIAGRQLFDPALIAEARRIAADG
                     GADVARAEQFVACAVSAFALSTEPIDRGWHEELTKVSAVAADIAGVRTTHINHLTPRV
                     LDIDELYRRMTARGITMIDAIQGPPAWHGPDVLLRQTSFRALAEPRRFRDADGTVSDG
                     TLRVRFGEVESRGVALTAKGRERYDAAMAASDPAAVWADHFPASHEGMAAAGLAYYHG
                     GDPSKPVVYEDFLPASAAGIFRSNLDTDTEAAEATDNSDYSLGWLAGQIGHHIHDPYE
                     LYEKVASS"
     gene            7320..7784
                     /locus_tag="VFC01_35710"
     CDS             7320..7784
                     /locus_tag="VFC01_35710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZN85005.1"
                     /translation="MVEASEHLDEIDRILVRELVADGRATLAHLAATAGLSVSAVQSR
                     VRRLESRGVVTGYTARIDPEAVGKMLSAFVAITPLDPSQPDDAPARLEHIPAIVSCHS
                     VAGEESYILLVRVESPRALEDLLQQIRTAANVRTRSTIILQTFYSGREIMPE"
     gene            7834..9159
                     /gene="lat"
                     /locus_tag="VFC01_35715"
     CDS             7834..9159
                     /gene="lat"
                     /locus_tag="VFC01_35715"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HZN85006.1"
                     /translation="MTAVLTPAVLRPDDVRDVLARSILADGLDFVLDIDRSSGSYLVD
                     ARTGERYLDMFTFFASSALGMNHPALADDEEFRAELAQAAVNKPSNSDVYSVPMARFV
                     DTFASVLGDPALPHLFFVDGGALAVENALKVAFDWKSRQNEAHGIDPELGTKVLHLRG
                     AFHGRSGYTLSLTNTDPNKVARFPKFDWPRIDAPYIRPGVDMDAVETESLRQARAAFE
                     AHPHDIACFIAEPIQGEGGDRHFRPQFFAAMRELCDEFDALLIFDEVQTGCGLTGTGW
                     AYQQLGVAPDVVAFGKKTQVCGVMAGRRVDEVRDNVFAVSSRINSTWGGNLVDMVRSR
                     RILEVIEADDLMVRAAKAGRYLLDRLEGLAAEFPGLVLDVRGRGLMCAFSLPSGAERD
                     ELIRRLWDRRVIMLGSGPDSVRFRPALTVSREEIDAAVGAVREVLTQLR"
     gene            complement(9143..9727)
                     /locus_tag="VFC01_35720"
     CDS             complement(9143..9727)
                     /locus_tag="VFC01_35720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HZN85007.1"
                     /translation="MSAHRSTIRAVRLRLYLVAGAVSGVAAYLLGVAPAWSLAIGALA
                     PGVLAAIPHFLRGAFAGLTTSDDVATGMSGMSGMSGTEFEDYVARVVRTSGVPVIMTS
                     VTGDWGVDIIVGTRPNRLAIQCKRQSRPVGTSAIQEVVAGAPMQECTRTMVVTNHEFT
                     PAARKLAELHGCELVGRTELPRLRSTIRRLTAAA"
     gene            9813..10493
                     /locus_tag="VFC01_35725"
     CDS             9813..10493
                     /locus_tag="VFC01_35725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016886848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS and ANTAR domain-containing protein"
                     /protein_id="HZN85008.1"
                     /translation="MTDELTDIEGQVPVEHALAGGEPQRVGWFRFYFADERWEWSPQV
                     ERMHGYEPGTARPTTDLVLSHKHPDDYGQVAATLDEIRRTSGAFSTRHRIVDTRGDVH
                     HVVVVGDQIFDDAGQVVGTHGFYVDVTPSMTEAHHEIVSEAVAEIAEARSGIEQAKGM
                     LMLVYRINADSAFELLKWRSQETNTKLRVLAEQIAKDFLELPYDEVLPARAVYDRLLL
                     TAHLRVGS"
     gene            10576..10956
                     /locus_tag="VFC01_35730"
     CDS             10576..10956
                     /locus_tag="VFC01_35730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85009.1"
                     /translation="MAAFRNPNDLRHDDAHVRDAVRFGLVVAVLAVAFLVTAAVWVST
                     CDGATADTAACGVPQRALLAIGAPVILLIGGLRAFVRTYQTWRNNEPWWAWQGAGWFL
                     FTLMLLVLTMSMPSLAGPAVFGGS"
     gene            11109..11417
                     /gene="usfY"
                     /locus_tag="VFC01_35735"
     CDS             11109..11417
                     /gene="usfY"
                     /locus_tag="VFC01_35735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein UsfY"
                     /protein_id="HZN85010.1"
                     /translation="MKGPKDPVDHARTTRPHAGESMKDNKVMPGLIVIGVALVLFVSC
                     LAAFATGHHDVGLALSSLAGAGFIMGAGWLLIEHMRVRRIEERWYAEHPDVDRQRPSS
                     "
     gene            complement(11473..11718)
                     /locus_tag="VFC01_35740"
     CDS             complement(11473..11718)
                     /locus_tag="VFC01_35740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZN85011.1"
                     /translation="MTDKKNTGPQEGVKGVVEDVKGKAKEAVGTVTGRDDLVREGKAQ
                     QDKADAQQDVAKKEAQAESARAGAKAAEKRQEAEQRP"
     gene            11870..12058
                     /locus_tag="VFC01_35745"
     CDS             11870..12058
                     /locus_tag="VFC01_35745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85012.1"
                     /translation="MKLVMNVFLIADLVFLDAHDVFARASGGPNQLIELDQDRFALRL
                     VVCRRRKSIRRLTSAPVV"
     gene            complement(12109..12450)
                     /locus_tag="VFC01_35750"
     CDS             complement(12109..12450)
                     /locus_tag="VFC01_35750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85013.1"
                     /translation="MQLSKLLGLRVVDAGHHAVGTVVDVRLTIAGDPQHNPSAPRVQG
                     LVISPRTTSSYLGYERTDANAPLMLAALMRWRHRGTFLAGWEDVARVGSDVLTLRPGY
                     TRRPAPLRDTD"
     gene            complement(12437..12802)
                     /locus_tag="VFC01_35755"
     CDS             complement(12437..12802)
                     /locus_tag="VFC01_35755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85014.1"
                     /translation="MTTLDARLHLLDRQLVDDDDNPVGIVDDLELDGVEPDTDIEPSA
                     AAPKVTALLSGHVLATRIFGGAPPRSRMQEIPWELVASVGVTVKLKPTDMSFDVQWTE
                     HWLRDHIIAHIPGGRHAAE"
     gene            complement(12799..14028)
                     /locus_tag="VFC01_35760"
     CDS             complement(12799..14028)
                     /locus_tag="VFC01_35760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HZN85015.1"
                     /translation="MKKVFAVALGILTAIGGFLDIGDLVMNAVVGSRFGLALAWVVVV
                     GIIGICLFAEMAGRVAVISGRATFEIIRERLGPRMAGANLTASFFINLMTLTAEIGGV
                     ALALQLATDVGRFMWIPVAAFAVWVVIWRVGFQIMENVTGLLGLCLIVFAVAVFLLHP
                     NWGDLAHQATAPVIPEKESAATYWYFAIALFGAAMTPYEVFFFSSGAVEERWTAKDLG
                     TARLNVMVGFPLGGILSIAIASCAAIVLLPSQIDVTSLSQMVMPVVTAGGKLALAFVI
                     IGIVAATFGAALETALSGGYILAQFLGWTWGKFRRPAEAARFHVVMFVAIVLGAAVLF
                     TGVDPIMVTEYSVVFSAIALPLTYLPILIVANDSQYMGAKTNGRLINAFGSVYLVIIL
                     AASLAAIPLMIVTGAGQ"
     gene            complement(14028..14492)
                     /locus_tag="VFC01_35765"
     CDS             complement(14028..14492)
                     /locus_tag="VFC01_35765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85016.1"
                     /translation="MAQQGDRLRRWRAIVLLGLCIVGLGLLLTRCPSNRDGMPGQLAQ
                     SMDETVTSARSGALAIDLWLQRRSTNQLTSVQISDARDEVVKAYKGITELKAEDPVDI
                     GRQRMLTQSMTDIIGQLNAASATLRTITTEPALESVRASLLASADALESGYR"
     gene            complement(14539..14745)
                     /locus_tag="VFC01_35770"
     CDS             complement(14539..14745)
                     /locus_tag="VFC01_35770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85017.1"
                     /translation="MQHSEEFPDDVPVADAVEQQRTFSEPVPDEEASAEPPDDVPLEA
                     APADWQEQLETVELDSDYDEVDRP"
     gene            14860..16023
                     /locus_tag="VFC01_35775"
     CDS             14860..16023
                     /locus_tag="VFC01_35775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZN85018.1"
                     /translation="MNIAMVSAQASPLAATSGLDASEQSVHVAGLSAALARCGHQVAV
                     YTRRDDPELPEIVETPQGYNVVHVAAGPPEPLADDELPRAVGPFARCLAAAWAESRPT
                     IAHAHSWISGIATQLVARQIDLPTIVRFHGLGTGPRLRLEAKLAKTATGVSAGCTDEA
                     FQLIRMGRPRPGISVIPSGVDVDEFTPNGLLAPRGEAPRIVSVGKLLPCNGFDTIIRT
                     LPFIPDAEVIVVVEDVSSEPDGEASRLHSLAEQLGVAERLKLHGAASVADKPALLRSA
                     DVVACAPSYESSGVVALEAMACGVPVVASAVGALRDTVVHEVTGYLVGQDPRKFAAAV
                     NALLRDSFLRRSLGAAGRDRAVARYTWDRIAADTARLYEKSISTCCEPAAAAL"
     gene            complement(16020..17036)
                     /locus_tag="VFC01_35780"
     CDS             complement(16020..17036)
                     /locus_tag="VFC01_35780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HZN85019.1"
                     /translation="MRLRRSALRGPGIRRIRRGRGFSYQHPDGTPVTDPATLQRIKDL
                     VIPPAWKKVWVCPHPNGHIQAVGTDAAGRRQYLYHQKWQEERNEEKFDRVLEMSAALP
                     NMRRRIAADLRRRGFDRDRVLGLALHLLDLGYFRAGSEQYAEENNSYGIATLLCEHVA
                     LQKQAVEFDYPAKSGVRRTLLIDDPEVVRSVRSLLRGRGRADRLLVCRNRSGWIDLHA
                     EDLNARFKELVGDEYTVKDLRTWHGTVLGAAAFVDADPPVNKTVIKRVESAVMKEVAE
                     ELGNTPAVARSSYVDPRVVEGYESGLTIAAGARRAARAKRPGERQAILEGSTARLIRK
                     VAKG"
     gene            complement(17127..17288)
                     /locus_tag="VFC01_35785"
     CDS             complement(17127..17288)
                     /locus_tag="VFC01_35785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85020.1"
                     /translation="MHPIARRSPLDDVADARPLRRILTTSGLVLAVVLLVALAIYAAA
                     FIMLAPMMQ"
     gene            17410..17844
                     /locus_tag="VFC01_35790"
     CDS             17410..17844
                     /locus_tag="VFC01_35790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZN85021.1"
                     /translation="MAEVANTDNGQLHSGRSVELRVAAKLENLAVLRTLVAAVGTFED
                     LDFDAVADLRLAVDEACTRLIRSAVPDSTLLLVVHPRDDAVVIDASTTCKSPDILAPG
                     SFSWHVLSSLTDEVRTFQNGQGAQEERVFGISMTTRRASSLQ"
     gene            17841..18629
                     /locus_tag="VFC01_35795"
     CDS             17841..18629
                     /locus_tag="VFC01_35795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="HZN85022.1"
                     /translation="MISSSAQGSSSRPNSEYADVADMFRELEGLPDSSAQFQRQRDRI
                     VERCLPLADHIARRFDGRGEPRDDLVQVARVGLVNAVIRFDVNAGSDFVSFAVPTIMG
                     EVRRHFRDNSWSVKVPRRLKELHLRLGAATADLSQRLGRAPTATELAAELDMDRDEVI
                     EGLVAGSSYNTLSIDSGGSGNEDAPAIADTLGDVDTSLDQIENREALRPLLAALPERE
                     RQVLLLRFFESLTQTQIAERVGISQMHVSRLLAKSLARLRDQLQ"
     gene            complement(18600..19040)
                     /locus_tag="VFC01_35800"
     CDS             complement(18600..19040)
                     /locus_tag="VFC01_35800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZN85023.1"
                     /translation="MSTPHSRPASPDALIEPTDCHTAHFATRWLQPSMAVITAHGELD
                     AANAQEFVDYSLRHAPHIDRLVLDLTGVDFFGTAGFSALHTLNVRCAAEKIEWALAPS
                     PAVSRLLQICDPDSALPISDSVDAALADVQGEPRRLLQLVPKSR"
     gene            19311..>19585
                     /locus_tag="VFC01_35805"
     CDS             19311..>19585
                     /locus_tag="VFC01_35805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZN85024.1"
                     /translation="MSKSTDAAGVLMAAGAGTRYGMPKVLADDGRWLRACVTALADGG
                     CDDVVVVLGAAVVDVPAPARAVIAHDWERGLSASVRAGMTAVDAAYAV"
BASE COUNT         3342 a         6354 c         6411 g         3478 t
ORIGIN      
        1 cccatgacgc cacggtgggc gtcgaccgca cccttgcggg tgagccagac gtccttgtcc
       61 gcgtgacgtt ctggcgccga gtagttgtgg tgcgtgttca ctctttccag ctcgacgccc
      121 tcgggatcgg cccccatcca atgcgcgaat gactgccgga accggtccat catctcctcg
      181 cggttggcga gcgcaaactg ttgggcccaa cgcaattcgg cgatgtagtt gtcgaactca
      241 gccgtaccct ccgacaggta ggcaaggtcg gcattgggca gcgcgacgcc catgtcgcgg
      301 cactgttgct tggccacagc gatgtgtcgg gtggcgatct tgtgcccgac gcctcgcgag
      361 ccggagtgca ggaacatcca gaccgcgtcg cctgcgtcga ggcatagctc gatgaaatgg
      421 tttccgccgc cgagggttcc caactgctcc ttccatttcg gcgaatgaga cagatcgaca
      481 tggccgtcgg cggccagttg ctccagatgc ttgatccggg cctgcgtgaa cgcgaaccgc
      541 cgcagcgact ggttgtagtt gcccgcactg agtgggatcg cggattcgag cgaattgcga
      601 agccgcgccg tgtctttgcc cgcgaggtcg tcggcggtga acttggtgcg ggcagcgatc
      661 atcccgcagc cgatgtcgac tccgaccgcc gccggaatga ccgcgccgag ggtcgggatc
      721 acggagccga cggccgcgcc cttgccgaga tgggcgtcgg gcatcaacgc cacatgcgga
      781 aagacgaacg gcagttccgc cgtctgcttg gcctgctcga tcgtggcgtc gtcgatctga
      841 ctggcgaaac tgaccacctt tccgccctcg agctgttgca tcatcgtcga tttccctccg
      901 tgtagcacca caaccttccg ccgcagaagg gggcggcgca aggagtttta gccgaacgct
      961 ctgtccaggc tcggccggcg aatcggttcg cgtccaggcg gttgcgacgg aggcggcgac
     1021 agcggcttgc ggcgccggag acgcaccttc gtcggggagc cggtgtgcag ttcgaggctc
     1081 tcgcccgcat ggcggatggt caacgcgctt tccgggccgt cgcgcagcgt gtaggcgacc
     1141 tcgtcgtggc ggacgtccac cgtcagccgg aaattcctcc agcgcagccg aaatcgcaag
     1201 catgagatgc catccggcag ctgggggtcg agcgcaagct caccgtcgtc atcacgcagc
     1261 ccgccgaagc cggcaaccag tgcggtccac gctccggcca gtgatgccat gtgcagtccg
     1321 tcgcgggtgt tgtggtgcag gtcgcgcaga tcgatcagcg ccgcttcgta aaggtaatcg
     1381 tgggccagtt ccaaatggcc gacctcggca cacatcaccg cctgcgtgca tgctgacagc
     1441 gacgagtccc tggtggtgcg tttctcgtag tagtcgacgt tgttggcctt ctgctccggc
     1501 gtgaactcgt ggctgagcca atgcatcgcc aggacgaggt cggcctgctt gaccacctgc
     1561 gacgggtaaa ggcgaacata gggctcgtac aacaacaagg gatacgacgt gttctcggtg
     1621 aagttccact cccgcagagt ggtgaagccc tcgcactgtt cgtgtacgcc gagttcctcg
     1681 tcgtagggga tgtgcacagc cgcggccgca tcgcgccacg ccgcggtttc ctcggaacca
     1741 acccccatcg cgtaggcggc atcgggatgc cgaccgcacg catcgacggc ggcgcgaaga
     1801 ttatgtgccg ccatgagatt ggtgaacacg ttgtcgcgca cgatggccgt gtactcgtcg
     1861 ggcccggtga cgccgtcgag gtgccagacg ccgtgccggt cgtgatgtcc caacgacatc
     1921 cacagccgcg cggtttccac cagcacccta agaccgcatt cctcctccag cgaatcatcg
     1981 cctgtgacaa ggcgataccg ctcgaaggcc atggcgatat cggcgttgat gtgccaggca
     2041 gccgtccccg caggccagta ggccgagcac tcctgtcctc ggattgtccg ccacggaaac
     2101 gcggcacccg caaggtccag ctcggccgcc cgttcgcgcg ccagatccag cgtcgacgcc
     2161 cgccagcgca acgcatcggc cgcggcgcgc ggcgcggtgt aggtgagtac tggaagaacg
     2221 aatccctcgg tgtcccaaaa tgtgtggccg tcatagccgg tgccagtcag gccctttccg
     2281 gggatggccc ggcgttcggc ccgcgcactg gcctgcaata cgtgaaagag gccgaaccgc
     2341 accgcctgct ggcaatccgc atcgccctcc acctcgacgt ccgcgcaatc ccagaattcg
     2401 tccagaaagt cccgctgtga ttcgagcaga ccctcccacc cgcagtactg ggcacccgcg
     2461 accgcgccga gtacctggtc gcgcaacgcc ggacgcgagc gcaggctgga ccatccatac
     2521 ccaaggaact tcacgatccg cagctgttgt ccggcgcgca acccgcagat gatggtcgtg
     2581 cgtgcccagt cctcgccggc atcggtgttc acttcgacgc ggcccggcac gtcgatttga
     2641 tggtccattc ccgcggccat catcaagcgg ctgctccggg ttcggtgcac gagcagcgct
     2701 ccccgctcac ccgcgtcgtg ctgcacggct tccaacgggt tcaacagcaa cgcggcgacg
     2761 cggggatcgg cggacggccg tggctgatct tcgttggcga ccagttcgga ttgcactgtg
     2821 aggcggacaa attcgtcgac cgcctctacc acgtactcga tcgcagccac actgcgctga
     2881 gccagcgaca ccagccgtgt cgtgcgcagt ctcacccgct tgcccgccgg agatgtccaa
     2941 cacgcgtggc gggtcagcgt gcctgcgcgc agatcgagca cgcgttcatg tgattccagg
     3001 tgtccgtatc ggacgtcgaa cggttcgtcg tcgacgagca ggcgcaagat cttgccgttg
     3061 gtgacgtcga ttactgattg gccgtcctcc ggatagctgt atcccgcttc ggcatacggc
     3121 agcgggcggg tttcgtagaa cgagttgaga tatgtccctg gcagtccata cggctcgccc
     3181 tcgtcgaggt tcccgcgcaa cccgatatgg ccgttggaca acgcaaacac cgactcggct
     3241 tgcgccaaca gattgagatc gagcttggtc tcgcgcacct gccacggttc gacgggaaag
     3301 gtctgttcgg tgatcatcgg ttcggcagca attcggccag atccgctacg acgatgtcag
     3361 cgcccttgcg gcgcaacgct tctgcctgac cgacccgatc cacaccgacg acgacgccga
     3421 agccaccgtt gtggcccgct tcgacgccgg ccaacgcgtc ttcgaagacg gccccttctg
     3481 cgggtttcac gtcgagcagt tccgctgcgc gcaggaacga gtcgggtgcc ggcttgcccg
     3541 cgatgtgttc ctcgcgcagg gtcacgccgt ccacccgttg ctgaacgaac ttgtccagcc
     3601 cagtgatctc gagcacctcg cgcgtgttgg agctcgatga cacgactgcg actgccagac
     3661 ccgccgcgct cgtcgcctcc aggtagcggc gcgaaccctc gaacactttg accccgtcct
     3721 tgcggagggt ctcgtggaac aagtcgttct tgtgattgcc caacccgtag accgtctcgg
     3781 ccgacgagtc gtcgtccgga tcaccgtctg gcagtgtgat gccgcgggcg tccagaaagg
     3841 accgcacccc gtcgtcgcgc ttcttgccgt cgatgtaggt cagatagtcg gcctcgatgt
     3901 cgaacgggac gaactgctcg ccactccgct ccgcggactt ccgcagatac gcgtcgaaca
     3961 tggccttcca cgccttcttg tgaacactcg cggtgtcggt gagcacaccg tcgaggtcga
     4021 acaggcaggc gcggatggcg tcgggtagac cgagcatggg gccctactga tgctgggctt
     4081 gtcggtgggt agcgacgtcc tcagatcggg cgggcacaag gcgtagcgcg agcagtccca
     4141 gaatcagcac gcacaacgcg ggcgccccaa atagcacgat cgctagcacc accccggtga
     4201 ctgagcctga cagtgctgtg gcgaatgcga acgccgccac caccaaggag acgacgcatg
     4261 tgaggaccgc gacgaccacg gcgacgacag aactggccag cacgccgtac cggggggctc
     4321 cgctcaccgc cagcacgacg aggacgggga tgccgatgat cgccgcggcc gtcaataagc
     4381 ccgtggccgg gccaagttga cgtgcggcgg gcatgatcag agccgcggcc gctatcgccg
     4441 ccaccaccac gccgaccaat gcaaatgcgc gcgaattgct gtgttgcatg gtcgtagagt
     4501 gacccgcttg tgccgcatca aaacgccgtg cgggcgccca tgttagccga agtgcacgcc
     4561 ctgcgccagc ggcagctcgg acgagtagtt gacggtgttg gtggcgcgac gcatgtacgc
     4621 cttccatgcg tccgaacccg actcgcgccc accgccggtc tgcttctcgc caccgaaggc
     4681 gccaccgatc tccgcacccg aggtgccgat gttgacatta gcaatgccgc agtccgaacc
     4741 gtcggccgcc atgaagcgct cggcttcccg aacgtcgagg gtgaatatcg ccgacgaaag
     4801 gccttgcggc actgcattgt tcaacccgat cgcctcgtcg aggtcgtcat aggtcagcac
     4861 gtacaggatc ggcgcgaacg tctccgcatg caccaccgcg gtctgcgacg gcatccgcac
     4921 cacggccggc gcgacgtaat acgcactctc gtcgccgagg tcgtggcgct cgccgccgaa
     4981 cacctcaccg ccgtcggcgc gggcctgttc gagtgcccgg accatgtcgc ggtacgcggt
     5041 ctcgtgaatg aggggaccga ccaatgttcc gtctgaggcg gggtcgccaa tcggtagctg
     5101 ccggtaggcg ttgacgatgc ggtcaaccag ctcgtcagcg accgaggaat gcacgatcaa
     5161 ccggcgcagt gacgtacaac gctggcccgc ggtgcctgcc gccgagaaca cgatgccccg
     5221 cacggcgaga tccagatcgg ccgatggcgt gacgatcgcc gcgttgttgc cacccagttc
     5281 cagcagcgcc ttgccgaacc gctgcgccac ccgcggaccg acctgctggc ccatccgtac
     5341 cgaccctgtg gcggaaagca gcgcgacccg cgcatcgtcg acgaggcgct cacccacctc
     5401 gcgaccgccc tggacgagcc gggcaatatg ctccgacgcc ccaacatcaa tggcggcgcg
     5461 ctcgatcagt gcttggcagg ccagcgcggt caacggtgtc agctccgatg gcttccacac
     5521 cacggtgtca ccgcagacca gcgcgatcgc ggtgttccac gaccagaccg caacggggaa
     5581 gttgaacgcg gtgatcacgc ccacgacgcc gagcgggtgc caggtctcca tcaaccggtg
     5641 ccccggccgc tcggacgcga tcgtcttgcc gtacagctgg cgcgacagac cgaccgcgaa
     5701 ctcgcagatg tcgatcatct cctgcacctc gccgagggcc tcggaggtga tcttgcctgc
     5761 ctcgatggtg acaagcgtgg cgagatcggc cttgtgttcg acgagcagct cgccgaggcg
     5821 ggccaccagc gcgcccctga ccggcgccgg ggtggtgcgc catgccgtaa acgcttgcgc
     5881 ggcttcggct atcgccgcat cggtttgttc gggcgtggtc tcggcaacgg tgaacagcac
     5941 ctcgccgctg atgggcgtgc tggcttgcag accgtggccg ccgggcgcgt ccagttcggt
     6001 ccgcgagcca acagcggtca gcgcgtcacg cgcctgattg cgcaggtcct cgactgtcgg
     6061 aagcttggat gtctgaacgg tggtcatgat gatgcgactt tctcgtagag ctcgtagggg
     6121 tcgtgaatgt ggtgtccgat ctgaccggct agccagccca ggctgtaatc cgaattatcc
     6181 gtcgcctcgg cggcctctgt gtccgtatcg aggttggacc ggaagattcc cgccgccgat
     6241 gctggcagga agtcttcgta gacaacgggt ttcgacggat caccaccgtg gtagtaggcc
     6301 agcccggcgg ccgccatgcc ctcatgggag gcggggaagt ggtccgccca gacggcggcg
     6361 gggtcagatg cggccatcgc ggcgtcgtag cgctcgcggc ccttcgcggt gagcgcgaca
     6421 ccacgcgact ccacctcacc gaagcgcacc cgcagggtcc cgtcagacac cgtgccatcg
     6481 gcatcccgga accggcgcgg ctcggccagc gcacgaaatg acgtctgccg caacaacaca
     6541 tccgggccgt gccaggcggg tggaccttgg atggcgtcga tcatggtgat gccgcgggcg
     6601 gtcatgcgcc ggtacaactc gtcgatgtcg agcacccgcg gtgtcaggtg attgatgtgc
     6661 gtggtgcgca ccccggcgat gtcggcggcc accgccgaca ccttggtcag ctcctcgtgc
     6721 cagccgcggt cgatcggttc cgtggagagc gcgaatgcgg acaccgcgca ggccacgaac
     6781 tgttcggccc gcgcgacatc agcgccgccg tccgccgcga tgcgcctggc ctcggcgatc
     6841 agcgcggggt cgaacagctg acgccccgcg atgaatcgct cgacccggcc gcacaagtcg
     6901 ggggagaaga accgcgcgtc ggcggtcgcg agcatcgagg taaacacccg gaacgggttg
     6961 tggcccagct cctctgtgtc gatcggccga aacgccgtgg acaccaccgg caccggcgat
     7021 gcagcgtcgc gcagatcgta gaagccgacc ggagacatgc cgaaggcggc gaacaggtcg
     7081 gcgacatcgg ccagttcgcg ggcgctgccg acgcggatcg ctccgtggcg ttcggcggtc
     7141 acccgctcag atgcgcccag ttcacggttc acctcggcgc tgacttcaac gagcgtgttg
     7201 taggcgggta cctcggcgcc atataggtgg gaaagcgccg cggcgaattt cgcccgcagc
     7261 tgccaggttt caatacgacg gctcatcgtg gacgccccgc attcggatac agtgcggcca
     7321 tggtggaggc cagcgaacat ctcgacgaga tcgatcgcat cttggtacgc gaactggtcg
     7381 ccgacgggcg ggcgacgctg gcacatctcg ccgcgaccgc cgggttgtcg gtgtcggcgg
     7441 tccagtcccg ggtgcgccgg ctcgaatcac gcggggtggt gaccggatac accgcacgga
     7501 tcgacccgga agccgtcgga aagatgcttt cggcgttcgt ggccatcact cctctcgatc
     7561 cctctcaacc cgatgatgcg cctgcccggc tcgaacacat accggccatc gtgtcgtgtc
     7621 actcggtggc cggtgaggag agctacatcc tgctcgtgcg tgtcgagtcc ccgcgagcgc
     7681 ttgaagactt gctgcaacag atccggacgg cggcgaacgt acgtacccga agcacgatca
     7741 tcttacagac tttttactct ggacgcgaaa tcatgccgga atagttcctg tcagtggggt
     7801 ggtcaaccgt aaataatttg ttaagatggt ctcatgactg ccgtcctgac acctgccgtc
     7861 ctgcgccccg acgacgtccg cgacgtcctg gcccgcagca tcctggccga tgggctggac
     7921 ttcgtcctgg acatcgaccg ctcatcgggc tcgtatctgg tcgatgcccg caccggcgag
     7981 cgctacctcg acatgttcac gttcttcgca tcgtcggcgc tggggatgaa ccacccggca
     8041 ctggccgacg atgaggagtt ccgcgccgag ttggcgcagg ccgcggtgaa caagccgagc
     8101 aactccgatg tgtacagcgt gccgatggcc cgcttcgtcg acaccttcgc aagcgtgctc
     8161 ggcgatcccg cgttgccgca cctgttcttc gtcgacgggg gagcgctggc cgtcgagaac
     8221 gcactcaagg tcgcattcga ctggaagagc aggcagaacg aggcccacgg gatcgacccg
     8281 gagctgggca ccaaggtgct gcacctgcgc ggcgcgttcc acggccgcag cggctacacg
     8341 ctgtcgttga ccaacaccga ccccaacaag gtcgcgcggt tcccgaagtt cgactggccg
     8401 cgcatcgacg cgccctacat ccgtcccggc gtcgatatgg acgccgtcga aaccgagtcg
     8461 ctgcggcagg cgcgcgcggc attcgaggcg cacccgcacg acatcgcgtg cttcatcgcg
     8521 gagccgatcc agggtgaggg tggcgaccgg cacttccgtc cgcagttctt cgccgcgatg
     8581 cgcgagctgt gcgacgagtt cgacgcgctg ctcatcttcg acgaggtgca gaccggctgc
     8641 gggctgaccg gaaccggttg ggcttaccag caattgggcg tcgcgccgga tgtcgtcgcg
     8701 ttcggcaaga agacacaggt gtgcggcgtc atggccggcc gccgggtcga cgaggtgcgc
     8761 gacaacgtgt tcgccgtcag ctccaggatc aattccacct ggggtggcaa cctggtcgac
     8821 atggtgcgat cgcggcgcat cctcgaggtc atcgaggccg acgatttgat ggtgcgcgcc
     8881 gccaaggccg gacgctatct gctcgaccgg ctggagggcc ttgcggccga gttccccggc
     8941 ctggtcctcg acgttcgcgg ccgcgggctg atgtgtgcgt tcagcctgcc gagcggcgcg
     9001 gagcgcgacg agctgatccg ccggctgtgg gatcgccggg tcatcatgct gggtagcggg
     9061 cccgacagcg tccggttccg gccagcgttg acggtgtcgc gcgaggagat cgacgccgcc
     9121 gtcggcgccg tgcgcgaggt actcacgcag ctgcggtgag ccgccggatc gtcgagcgca
     9181 gccgagggag ctcggtccgg ccgacgagct cgcagccgtg cagttcggcg agtttgcgtg
     9241 cggcgggcgt gaattcgtgg ttggtgacca ccatggttct ggtgcattcc tgcatgggcg
     9301 cgcccgccac cacttcctgt atggcgctgg tgccgacggg gcgggactgc cgcttgcatt
     9361 ggatggcaag gcgattgggg cgtgtgccga caatgatgtc gacaccccag tcgccggtaa
     9421 ccgacgtcat gatcaccgga acaccggacg tccgtaccac gcgtgcgacg tagtcctcga
     9481 actccgtccc cgacatcccc gacatccccg acatccccgt ggccacatca tcggaggtgg
     9541 tcaagcccgc gaaggcgcca cgcagaaagt gcggaattgc cgccagtacc ccaggcgcga
     9601 gggctccgat cgccaggctc cacgccgggg ccacgccgag aaggtatgcg gcgacgcctg
     9661 ataccgcgcc cgcgacgagg tagagccgaa gcctcacggc ccgaatggta gatcggtggg
     9721 ccgacatcac cacgcaccat cgtctgtaca aaaaacgaat gggtatatct gctccgatgc
     9781 cgtgtggttg ttagccaggt taacctgcac acatgaccga tgagctgacc gatatcgagg
     9841 ggcaggtgcc cgtcgagcac gcattggccg ggggcgaacc ccagcgcgtc ggttggttcc
     9901 ggttctattt cgccgacgaa cggtgggagt ggtcgccgca ggtcgagcgc atgcacggct
     9961 acgagcccgg cactgcgcgg cccaccaccg atctcgtgct gtcccacaag caccccgacg
    10021 actacggcca ggtcgccgcc accctcgacg agatccggcg tacgtccggc gcgttctcca
    10081 cccggcaccg catcgtcgac accagaggcg acgttcacca cgtggtcgtg gtcggcgacc
    10141 agatcttcga cgacgccggc caggtggtgg ggacgcacgg cttctatgtc gacgtgacgc
    10201 cgtcgatgac ggaggcccac cacgagatcg tcagcgaggc ggtggccgag atcgccgagg
    10261 ctcgcagcgg tatcgaacag gccaagggca tgctgatgct ggtctaccgg atcaatgccg
    10321 actcagcgtt tgagctgctc aaatggcggt cccaggagac caacaccaag ctgcgggtac
    10381 tcgccgagca aatcgcgaag gactttctcg aacttcccta cgacgaggtg ctgcccgcgc
    10441 gcgccgtcta cgacagattg ctgctgacag cgcatttgcg cgtcggatcc tgaggtttat
    10501 tcgcccttcg gacagggaag tctttcccag gtctcaccga gacgtctgag tagcgaatcc
    10561 aggagtttgg ctagggtggc tgcgtttcgc aatcccaatg acctgcggca cgacgacgcc
    10621 cacgttcgcg acgccgtgcg cttcggccta gtggtcgcgg tgctcgcggt ggcgttcctc
    10681 gtcacggccg ccgtatgggt cagcacctgt gacggtgcga ccgccgacac cgcggcctgc
    10741 ggcgtccctc agcgggcgtt gttggcaatc ggtgcgccgg tcatcctgtt gatcggtgga
    10801 ctgcgagcct ttgtccgcac gtatcagacc tggcgcaaca acgagccgtg gtgggcgtgg
    10861 cagggcgcgg gctggttcct gttcaccttg atgctgttgg tgctgacgat gagcatgccg
    10921 tcgttggccg ggcctgccgt gttcggcggg agctgacgcg tacccggcgt gctgcggggt
    10981 gagcattacc cggcctacct gtgacgtcac atttcagtga cggtttctcc cacaacggtg
    11041 tcgggtagtt ctaccgttga aatcagccca tgtttggcgg gcgtaggtat gccgagagag
    11101 gagtaccgat gaagggcccg aaggatcctg tcgaccacgc gaggacaacg cggccgcacg
    11161 ccggcgagtc gatgaaggac aacaaagtaa tgccggggct cattgtgatc ggcgtcgcgc
    11221 tggtgctttt cgtgtcgtgc ctcgcggcgt ttgccaccgg ccaccacgac gtcgggttgg
    11281 ccctgtcgtc cctggccggc gccgggttca tcatgggcgc ggggtggctg ctgatcgagc
    11341 acatgcgggt acgccgcatc gaagagcgtt ggtacgcaga acatcccgac gtagaccggc
    11401 agcgccccag tagctgacga cacaagataa aggcccagcc gatttccggc tgggcctttt
    11461 ctttcgaaac tgttacggcc gctgctcggc ctcctggcgc ttctcagcgg ccttggcgcc
    11521 ggcgcgagcc gactcggcct gcgcttcctt cttggcgacg tcctgctgtg cgtcggcctt
    11581 gtcctgctgg gccttgccct cgcggaccag gtcgtcacgg cctgtcacgg tgccgaccgc
    11641 ttccttggcc ttgcccttga cgtcctcgac gacgcccttg acgccttctt gcgggccagt
    11701 gttcttcttg tcggtcatgc ttaccttccg cgtgtaactg gatcggtcgt tgcccacttc
    11761 gtgagccgcg ggcccttgcg ggccgcacga agggaatctc cccacgcggt cagagcgttc
    11821 ccgaggatcc gactggctaa accttcccgg gtcgcgcacc gccccggtgg tgaagctggt
    11881 catgaacgtc ttcctgatcg ccgacctcgt gtttctcgat gcccatgacg tcttcgcgcg
    11941 cgccagtggt ggcccgaatc agctcatcga gcttgaccag gatcgctttg ctctccggtt
    12001 ggtggtgtgc cggcgcagga agagcatcag aaggctcacc agtgcgcctg tggtgtagac
    12061 gaaggtttgc cacctctgtc gtctgccatc gccgccgctt cccggaactc agtcggtatc
    12121 gcgcaatggg gccggacgcc gggtatatcc gggcctcagc gtgagcacgt cggatcccac
    12181 ccgcgccacg tcttcccacc cggccaggaa cgtgccgcgg tgtcgccagc gcattagcgc
    12241 cgccagcatc agcggtgcgt tggcgtcggt gcgctcgtac ccgagatatg aggaggtcgt
    12301 ccgcgggctg atcactagtc cctgcacccg cggcgccgac ggattgtgtt gtggatcacc
    12361 ggcaatcgtg agccgtacat ccaccacggt gcccacagca tgatggcctg cgtcgaccac
    12421 acgcaacccg aggagtttac tcagctgcat gccggcctcc cggaatgtga gcgatgatgt
    12481 ggtcgcgcag ccagtgttcc gtccactgca cgtcgaagct catgtcggtg ggcttcagtt
    12541 tgaccgtcac gccgaccgaa gccaccaatt cccaagggat ctcctgcatg cgcgaacgcg
    12601 gcggcgcgcc cccgaagata cgggtggcaa gcacatgtcc cgacagtagc gccgtgactt
    12661 tcggcgccgc agcgctcggc tcgatgtcgg tgtcaggttc cacgccatcc aattcgaggt
    12721 cgtcgacgat gcctaccgga ttgtcatcgt catcgaccag ttggcggtcc agtaggtgca
    12781 gccgcgcgtc aagtgtggtc attggcctgc tcccgtcacg atcatcagtg gaatggcggc
    12841 cagcgaggcg gccaggatga taacgaggta caccgaacca aacgcgttga tcagccgtcc
    12901 gttcgtcttg gcccccatgt actgagaatc gttggccacg atgagaattg gtaggtaggt
    12961 cagcggcagc gcgatggcgg agaagacgac tgagtactcc gtgaccatga tcgggtcgac
    13021 accggtgaag agcactgctg cgccgagcac gatagcgacg aacatcacca cgtggaaacg
    13081 tgcggcctcc gcgggccgac ggaatttgcc ccacgtccag ccgagaaatt gggcgaggat
    13141 gtagccgccc gacagagcgg tttccagcgc cgcgccgaag gttgcggcca cgatgccaat
    13201 gatcacaaag gcgagtgcca acttaccgcc ggcggttacg accggcatca ccatctgtga
    13261 cagcgaagtc acgtcgatct ggctgggcag caagacgatc gccgcgcagg aggcaatggc
    13321 gatcgagaga attccgccca atgggaaacc gaccataaca ttgagccggg cagtgcccag
    13381 atccttggcg gtccaacgct cttcgacagc gccggacgag aagaagaaaa cctcatacgg
    13441 cgtcattgcg gcgccgaaca acgcgatcgc gaaataccaa taggtggcgg cagattcctt
    13501 ctccgggatg acgggggcgg tcgcctggtg cgcgaggtcg ccccaattcg ggtgcagcag
    13561 gaacaccgcg acggcgaaga cgatcagaca caagccaagc agtccggtga cgttttccat
    13621 gatctgaaag cccacccgcc agatcaccac ccataccgcg aaagcggcga cgggaatcca
    13681 catgaaccga ccgacgtcgg tggccagctg caaagcgagt gcgacgccgc cgatctcggc
    13741 cgtcagcgtc atcaggttga tgaagaaaga agcggtgaga ttcgcgccgg ccatccgcgg
    13801 gcccaatcgt tcacggatga tctcgaatgt agctcggccg ctgatcacgg cgacccgtcc
    13861 ggccatttcg gcgaacagac agattccgat gattccgacc accacgaccc aagccagcgc
    13921 caagccgaac cgggaaccga cgaccgcgtt catcacgagg tcgccgatgt cgagaaatcc
    13981 accgattgcg gtgaggattc caagcgctac tgcgaagacc ttcttcatca gcggtatccc
    14041 gactccagcg cgtcggccga cgccagcagg ctggcgcgta ccgattccag tgcgggctcg
    14101 gtggtgatcg tacggagcgt cgcggatgcg gcgttgagct ggccgatgat gtcggtcatg
    14161 gactgcgtca gcatccgctg acgtccaatg tccaccgggt cttccgcctt cagctcggta
    14221 attcccttgt aggccttgac cacttcgtca cgggcatcgg agatctgcac gctggtcagc
    14281 tgattggtcg accgacgctg cagccacagg tcgatggcca gggcgccgct gcgtgcggac
    14341 gtcaccgtct cgtccatgga ttgagccaac tggccgggca ttccgtccct gttggacggg
    14401 caccgggtca acagcagccc cagtccgacg atgcacagcc cgagaaggac tattgcccgc
    14461 caacgccgca ggcggtcgcc ttgctgtgcc atcgcggctc ctgtcagccg gctaacaggt
    14521 cccgaccttt aggctccgtc atggccggtc gacctcgtca taatccgaat ccagctcgac
    14581 cgtttccagc tgttcctgcc agtcagcagg tgccgcctcg agcggcacgt catcgggcgg
    14641 ttcggcggac gcctcttcgt cgggaaccgg ttcgctgaac gtccgttgtt gctcgacggc
    14701 atcggcgacg gggacgtcgt cgggaaattc ttcgctgtgt tgcacggcgt cggaataccc
    14761 cgcgcactgt gaccaaaaac tagcgcgaag cgactgcgtt tcgtatgccg gacaacgtgg
    14821 gtacacgttc gtgtggccgt tcacaccgcc gcgacgtcga tgaatatcgc catggtttcg
    14881 gcgcaagcga gcccgctcgc ggccacgagt gggctcgatg cgagcgagca gagtgtgcat
    14941 gtggccgggt tgtcggctgc gcttgcacgc tgtgggcacc aagtagctgt gtatacccgt
    15001 cgagacgatc ccgagctgcc tgagatcgtt gaaacaccgc agggctacaa cgtggttcac
    15061 gtggccgccg gtccgccgga gccgcttgcc gacgatgaac tcccgcgcgc tgtggggcca
    15121 ttcgcccgtt gcctggctgc ggcctgggcc gagagccgcc caactatcgc gcacgcccac
    15181 tcctggatct cgggcatcgc gacccaactc gtggctcgcc aaatagacct gcccacgatc
    15241 gtgaggtttc atgggttggg cactgggcct cggctgcgcc tggaggcaaa gctggcgaag
    15301 accgcgacgg gcgtctcggc tggttgcacc gacgaagcgt tccaactgat tcggatgggc
    15361 aggccacgcc cgggcatctc ggtgattccg tcgggcgtag atgttgacga gttcactccc
    15421 aacggactcc tagcaccgcg gggcgaggcg ccacgcatag tcagtgtggg aaagctgttg
    15481 ccgtgcaacg gattcgacac aatcattcgt accctgccat tcatccctga cgccgaggtc
    15541 atcgttgtcg tcgaggacgt ttccagcgag cccgatggcg aggcgagtcg gctacacagt
    15601 ctcgccgaac agttgggcgt cgctgagcga ctaaaacttc acggggcggc cagcgttgcc
    15661 gacaaacctg cacttttgcg atccgcggat gtcgtcgcat gtgctccttc ctatgaatcg
    15721 tccggcgtcg tggcgctgga agcgatggcc tgtggtgttc cggtggtggc ctccgcggtg
    15781 ggcgcgctgc gtgacaccgt cgtccacgag gtgacgggtt atctggttgg ccaggatccg
    15841 aggaagttcg ctgcggcggt gaacgcgctg ctgcgggatt ccttcttgcg gcgcagtctc
    15901 ggtgccgcag gaagagaccg cgctgtcgcg cgatacacgt gggaccggat cgccgcggac
    15961 accgcgcgac tctacgagaa gtcgatatcg acgtgctgcg agccggcggc agctgctctc
    16021 taacccttcg ccacttttcg gatcagccgc gcggtgctgc cttccaggat ggcctgccgc
    16081 tcgccgggtc gcttggcccg tgccgcccgc cgcgcgccgg cggcgatggt caggccggat
    16141 tcgtagccct cgaccacccg cggatcgaca tacgacgacc gggcgacggc gggggtgttg
    16201 ccgagttcct ctgctacttc cttcattacg gcagactcca cacgtttgat cacggttttg
    16261 ttcaccggtg ggtccgcatc gacgaacgcc gcggcgccca acaccgtgcc gtgccaggtg
    16321 cgtaggtcct tgaccgtgta ctcgtcgccg acaagttcct tgaaccgcgc gttgaggtct
    16381 tcggcgtgca ggtcgatcca gccagacctg ttgcggcaca ccaatagtcg atcggcgcgg
    16441 ccgcggcccc gcagcaacga tcgcacggac cgaaccacct cggggtcgtc aatcagcagt
    16501 gtgcggcgca ccccgctctt ggcgggatag tcgaattcca cggcctgctt ctgcagcgcg
    16561 acgtgttcgc acagcagtgt ggcgatgccg taggagttgt tctcctcggc gtactgttcg
    16621 ctgccggccc tgaagtagcc gaggtcgagc aggtgcaggg cgagcccgag cactcgatcg
    16681 cggtcgaaac cgcggcggcg taggtcagcg gcgatgcgcc ggcgcatgtt cggcaacgcc
    16741 gccgacatct ccagcacccg gtcgaacttc tcctcattgc gctcttcctg ccacttctgg
    16801 tggtagaggt actggcgtcg accggcggca tcggtaccca ccgcctggat atgaccgttc
    16861 gggtggggac agacccagac cttcttccac gcgggaggga tgacgaggtc cttgatgcgt
    16921 tgcagggtcg ccgggtcggt caccggtgtg ccgtctggat gttgatagga gaacccgcgc
    16981 ccgcggcgga tccggcgaat tccggggccg cggagggcgc tacgtcgaag tcgcatgttt
    17041 ggcgggcgac cacggcgggg tattgcacgt ggaccatggt gaacgggaca tcgacagacg
    17101 ctgcgccgaa actctggcca tcggcgtcac tgcatcatcg gcgccagcat gatgaacgct
    17161 gccgcgtata tcgccagcgc gaccaggagc actacggcga gcacgagtcc gctggtggtg
    17221 agtatccggc gcagcggccg ggcatcggcc acgtcgtcga ggggacttct gcgcgcaatt
    17281 gggtgcatga cgaggggtta cccggcggca agcgttgcca cacgtcagtg cccaccggtg
    17341 atcggagcgc cggtggttag ctggggatcg agctggggta gtctcgcgaa aatagtgccg
    17401 ggaggtcaca tggccgaggt cgcgaacaca gacaatgggc agctccacag cggcaggtcg
    17461 gtggaactcc gggtcgcggc caagctggaa aacctcgcgg tgttgcgcac cctggtggcc
    17521 gccgtcggca cgttcgagga cctcgatttc gacgccgtcg ccgatctgcg tctcgccgtc
    17581 gatgaagcgt gcacccggct gatccggtcg gccgtgcccg actcgacgct gttgctggtg
    17641 gtccacccgc gagacgacgc cgtcgtcatc gacgcatcga ccacatgtaa gagcccggac
    17701 atcctggcgc cgggcagctt cagttggcat gtgctgagct cgctgacaga cgaggtacgc
    17761 accttccaga atggacaggg cgcgcaggag gagcgggtgt tcgggatctc catgacgaca
    17821 aggcgagcga gttcgctgca gtgatctcgt cgtcggccca gggttcgtca tcacgaccga
    17881 actctgaata cgcggacgtc gctgacatgt tccgcgaact cgaaggtctg cccgactcct
    17941 cggcgcagtt ccaacgtcag cgcgaccgga tcgtggaacg atgcctgccg ctggccgacc
    18001 acatcgcccg ccggttcgac gggcgcggag aaccgcgcga cgatctcgtg caggtggccc
    18061 gcgtgggcct ggtcaacgcg gtgatccgat tcgatgtcaa cgccgggtcg gatttcgtgt
    18121 cgttcgcggt gccgaccatc atgggcgagg ttcgcaggca cttccgcgac aacagctggt
    18181 cggtcaaggt gcctcgccga ctcaaggaac tgcatctgag attgggcgcc gcgacggccg
    18241 acctgtcaca gcggctcggt cgcgcgccga ccgcgacgga actcgctgcc gagctggaca
    18301 tggaccgcga cgaggtgatc gagggcctgg tcgcgggcag ctcgtacaac acgctgtcga
    18361 tcgacagcgg cgggagcgga aatgaggatg cgcctgcgat tgccgacacg ctcggtgatg
    18421 tcgataccag cctggatcag atcgagaacc gggaagcctt gcggcccttg ctcgctgcgc
    18481 tgccggagcg tgagcggcaa gtgttgctgc tgcggttctt cgagtcgctg actcagacgc
    18541 agatcgccga gcgggtcggc atctcgcaga tgcacgtgtc ccgtttgttg gcgaaatccc
    18601 tagcgcgact tcgggaccag ttgcagtagc cggcgcggct caccctgcac atccgcaagc
    18661 gcggcgtcga cactgtcgga gatcggcaac gccgaatccg gatcgcagat ctgcagcagt
    18721 cggctgacgg cggggctcgg cgccaaggcc cactcgatct tctcggcggc gcagcggaca
    18781 ttcagggtat gcagggccga aaagcccgcg gtaccaaaaa agtccacgcc ggtcagatcc
    18841 aggaccaggc gatcgatatg cggggcatgc cgcaacgagt agtcgacgaa ctcctgcgcg
    18901 tttgcggcgt cgagttcacc gtgtgcggtg atcaccgcca tcgatggttg cagccaccgg
    18961 gtggcgaaat gcgccgtgtg gcagtcggtg ggctcgatga gggcgtccgg cgaagccgga
    19021 cggctatggg gggtcgacat tgtgactcca tcagtcggaa tacgtgattc gtactgtggt
    19081 tcacgtcaga ttttctaggc cctgcgtcct caaagcctcg ctacagggcg tgggctccac
    19141 cctgctcgtc tcttgacgtt tccagctggc tgtgaactca acctggcttg accatacgcc
    19201 gacgggcggt cgcggggcaa tcgatctagc acgaatttcg tttttccagc tcaggtgtgc
    19261 tattcgcggt tgcggaggtg tcgcgcatct gacaggttca gactgttcgc atgtctaaga
    19321 gcaccgatgc cgccggtgtg ctgatggccg cgggggcggg gacccgatac ggcatgccga
    19381 aggtgctcgc tgacgacggt cggtggctgc gtgcctgcgt gacggcgtta gccgacgggg
    19441 gctgcgacga cgttgtcgtt gtgttgggcg ccgcggtggt cgacgtgcct gcgccggcgc
    19501 gggcggtgat cgcgcacgac tgggagcgag gcctcagtgc gtcggtgcga gcgggtatga
    19561 cggcagtcga cgccgcctac gccgt
//
LOCUS       DASRJA010000794         5600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3192923,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000794 DASRJA010000000
VERSION     DASRJA010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3192923"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VFC01_35810"
     CDS             <1..347
                     /locus_tag="VFC01_35810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HZN85025.1"
                     /translation="GMSGQTMPPMSGMASPPASASATTADAPAPQGGTAVSISDFKFN
                     PATLTVPVGATVTWTNKDEEPHTIAAKDGSFHSPGMDTNGTYSFTFTTPGSYDYICSI
                     HPFMTGTVVVTK"
     gene            344..1276
                     /locus_tag="VFC01_35815"
     CDS             344..1276
                     /locus_tag="VFC01_35815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016887673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZN85026.1"
                     /translation="MSTEHNPEGMSRRQLIRHSAWFGAAVVLTVAGGEVISHVAGSSG
                     AAQQATPALRFAQISDSHIGFKGPANTNVTDTFNHAIGQINGLGYTPDFVIHTGDLTH
                     LATPDQFDQVKHMLGELNTPHVFTVPGEHDSTDDAGQKYRQAFGAGTRGDGWYSFDVA
                     GVHVIGLVNTLNMQKLGHLGADQLEFVEKDVAGLSSDTPIIVFSHIPLFAMYPDWGWG
                     TEDAAQALSYLKRFSSVTCLNGHVHQLFSKTEGNVTFYSATTTAYPLPAPGQGPAPKP
                     VTLPAGKLRDALGIREVSYTKGQQALALKDQKLT"
     gene            1273..1713
                     /locus_tag="VFC01_35820"
     CDS             1273..1713
                     /locus_tag="VFC01_35820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85027.1"
                     /translation="MKIMDLAIRTAISATLAVSGVVHAYLYIHGYRDIPAVGPAFLVQ
                     GSVFCALAVLILAGGPGWLQLFAAIGAAGSLVAFALSRTVGLFGFTETGWQPAPYAAI
                     TVVVEVLTLVLVVTAALRQRPRASKYLLPDLSPDGAAEPSARQG"
     gene            1741..2514
                     /locus_tag="VFC01_35825"
     CDS             1741..2514
                     /locus_tag="VFC01_35825"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZN85028.1"
                     /translation="MGDREVTADPNDRFDLSRPNMIGLAAAVGMGVAVSSPASAGPSP
                     TGLGHPITPWQSRPRSLGAAGARPQDDDRIAELVPPPPLDVPLDAAPEDHPMRKTFAA
                     VPPAPESALAAQTISWRRADIGAANGHGNARFLARVLSPISLEGKANGVQLLGPATID
                     LIFQEQANGTDLVLAMPARWGIGYALPKPEAVPDIPNERICYWGGWGGSMAVMNPDRR
                     TTFAYVMNKMGQVTSAGTERTQKYTRLIYQALGSPTGNT"
     gene            2769..4250
                     /locus_tag="VFC01_35830"
     CDS             2769..4250
                     /locus_tag="VFC01_35830"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZN85029.1"
                     /translation="MAGGKPNVAVIGAGPGGIAMGIQLAAGGYDFTIFDRNDGFGGTW
                     RNNTYPGAACDVPSHFYSYSFALNPRWSKTFANQPEILAYLEKVAAENRLTDHLEART
                     RVRTLRWSDIDRRWTVQTDDGATHQFDAVVTAVGMLDVPNIPDIPGAKRFRGRAFHSS
                     NWDHTKTTAGERVASIGTGASAIQYVPEIAPDTAQLTVFQRTPIWVSPRYDEPFTPEQ
                     HELFERDPVEALKVRDAAFEAYESANFGVDSAMTKELTEIAHAYLTRKVNDPDLRAKL
                     TPSYPVGCKRPLQSRAWFPTFALPNVRLETSPIVEFTERGLRTANGAEHEVDTVIYGT
                     GFHAADYLRSLDVYGTEGRRLRDDWRDGAEAYLGTVVPGYPNLFTLYGPNTNGVTSII
                     YILEAQTEFVRRTLDDMTLRRVQAIDVKRGVHDAYNDDIQRAMQDTVWLANCNNYYRH
                     PNGKVVTQFPYSGTTFVSMLDQVGLDVFNWMEATACQESTTLR"
     gene            4223..4663
                     /locus_tag="VFC01_35835"
     CDS             4223..4663
                     /locus_tag="VFC01_35835"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZN85030.1"
                     /translation="MPGIHHTAIVTADVERSVRFWCDGLGLTELMDYTFTGDWPTLFS
                     ARTDRLRSIFLGDPDTPDTGIVELVQFDDTGLGAPPPDEPAFGFFLLSLQRDVDATLA
                     ALAGLGFDDGVRRIEMPAPGGKTVAMAVVTAPDGVRVELIGPPQ"
     gene            4660..5391
                     /locus_tag="VFC01_35840"
     CDS             4660..5391
                     /locus_tag="VFC01_35840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007169361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN85031.1"
                     /translation="MIAVVTGGGSGIGRAVVDRLRNAGADVVVWDIKDGDIRCDIKDP
                     EWVSAAIEQTVADHGTPDRLVACAGIGASGLLLEQTPADWQRVIDTNLTGTWLTMSAV
                     ARAMVDAGSGGSMVAVSSISGTLADRDMGAYCVSKAGIGMLVRVAAAEWGAHGIRVNA
                     VGPGVTRTPKLAKPEQLPGWVEGLTERTALGRLGEADDVAEAIVAVLEMSWVTGQIMF
                     ADGGLALHSPIDAYGQVQRVTRGGR"
     gene            complement(5388..>5600)
                     /locus_tag="VFC01_35845"
     CDS             complement(5388..>5600)
                     /locus_tag="VFC01_35845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6611 family protein"
                     /protein_id="HZN85032.1"
                     /translation="GAMVMAGHHDPVSVAVRHRLENLAGALMEADEQLARGEISATAH
                     EMTWWRVYDQMAPSASPGMHPSGTGA"
BASE COUNT         1044 a         1906 c         1706 g          944 t
ORIGIN      
        1 cgggtatgtc cggtcaaacc atgcctccca tgtccggtat ggcgtcgcca ccggcttcgg
       61 catcggcgac tactgccgat gcgcccgcac cgcaaggagg gacggcggtc agcatcagcg
      121 atttcaagtt caacccggcg acgctgaccg tcccggtcgg cgcgacggtc acctggacca
      181 acaaggacga agagccgcac accatagccg ccaaggacgg ctctttccac tcgccgggaa
      241 tggacaccaa cggcacctac agctttacgt tcaccacgcc cggctcttac gactacatct
      301 gctcgatcca tccgttcatg accggcaccg tggtggtgac caaatgagta ccgagcacaa
      361 cccggagggc atgagtcgac gacagctgat ccgccacagc gcctggttcg gcgccgccgt
      421 tgtgctcacg gtcgcaggcg gtgaagtgat ctcgcacgtc gccggatcat ccggcgctgc
      481 acagcaggcg acacccgcac tgcggttcgc tcagatcagc gacagccaca tcggattcaa
      541 gggcccggcg aacaccaacg tgaccgatac gttcaaccac gccatcggcc agatcaacgg
      601 cctcggctac acacccgatt tcgtgatcca caccggcgat ctgacccacc tggccacgcc
      661 ggatcagttc gatcaggtca agcacatgct gggcgagctg aacacgcctc acgtgttcac
      721 cgtgcccggt gaacacgact cgacagacga tgcgggacag aagtatcggc aagccttcgg
      781 cgcgggcacc cgtggcgacg gttggtacag cttcgacgtc gcaggcgtgc acgtcatcgg
      841 gctggtgaac acgctgaaca tgcagaagct cggccatctc ggcgccgacc agctcgagtt
      901 cgtcgagaag gacgtcgcgg gcctgtccag cgacaccccg atcatcgtgt tcagccacat
      961 cccgctgttc gccatgtacc cggactgggg atggggcacc gaagacgccg cgcaggccct
     1021 gagctacctc aagcggttct cgtcggtgac atgcctcaac ggccatgtcc accaactgtt
     1081 ttccaagacc gagggcaatg tgacgttcta cagcgctact acgaccgcct atccgctgcc
     1141 ggcgccgggt caaggtcccg cacccaaacc ggtgacgctg cccgccggaa agctgcgcga
     1201 tgcgctgggc atccgcgaag tcagttacac caagggccag caggccctcg ccctgaaaga
     1261 tcagaagctc acatgaagat catggatctg gcaatcagaa cagcgatcag cgcgaccctg
     1321 gcggtcagcg gcgtggtaca cgcctacctc tacattcacg gctaccgtga catccccgcg
     1381 gtagggcccg ccttcctggt gcagggcagc gtcttctgcg cgctcgcggt gctgatcctg
     1441 gccggcggac cgggctggct gcaactgttc gccgcaatcg gggcagcagg ctcactggtc
     1501 gcattcgcgc tgtcgcgtac cgtcgggctc ttcggcttca ccgaaaccgg ctggcagcca
     1561 gcgccatacg ctgcgatcac cgtcgtcgtc gaagtgctga ccctggtgct ggtagtcacg
     1621 gcggccctgc gccagcgtcc acgggcatcg aagtaccttc tgcctgactt gagccccgac
     1681 ggcgctgctg aaccatcagc tcgccagggt tgaccgactt cacgcgggga ggcggatccg
     1741 atgggtgatc gcgaggtgac cgcagatccg aatgatcgct tcgatctgtc ccggccaaac
     1801 atgatcggct tggcggcagc cgtcggcatg ggcgtcgccg tgtcctctcc cgcatcagcc
     1861 ggtcccagcc cgactggttt aggtcatccg atcacgccgt ggcaatctcg tccacggtcc
     1921 cttggggctg ccggagcgcg cccgcaagac gatgaccgca tcgccgagct ggtcccccca
     1981 ccgcctttgg atgtgccgct ggacgcggcg cctgaggacc atccgatgcg caagacgttc
     2041 gccgctgttc cgcccgctcc cgaaagcgca ttggcggcgc agacaatctc atggcggcga
     2101 gctgatatcg gggcggccaa cggtcacggc aacgcacgtt tcctagcccg tgtgctgtca
     2161 ccaatctcgc tggaaggcaa ggccaacggc gtgcaattgc tgggtccggc gacgatcgac
     2221 ctgatctttc aggaacaagc caacggcacc gacttggtgc tggccatgcc agcacgctgg
     2281 ggtatcggct acgcactacc caagccagaa gcggtacccg acattcccaa cgaaaggatc
     2341 tgctactggg gcggctgggg cggctcaatg gcagtgatga atcccgaccg ccgcaccacc
     2401 ttcgcctacg tcatgaacaa gatgggccaa gtcacctcag ccggcaccga acgcacccag
     2461 aaatacacca ggctcatcta ccaggcgctc ggctcgccga ctggtaacac ataggcccca
     2521 tctgaggatc cgccacgagt atgagaaaga gcgcttcgcc atttccgtga gaattctgca
     2581 acgtcgcagg tgagggaatc gctgtccgcc acgcagtttg agaagcgcag tggtggtggc
     2641 agccgccgcc gaagccgaca gctgtgttcg cgcttttcgc attgcgcgcg tacgcccgcc
     2701 gcactggagg ctagacaacc cgagacagca tcgttacgta tcacgctata tatgtaatga
     2761 ttgcaaagat ggcaggcggg aagccgaacg tcgcggtgat tggtgctggt cccggcggca
     2821 ttgcgatggg aatccagctc gccgccggtg gctacgactt caccatcttt gaccgcaacg
     2881 acggcttcgg cggcacctgg cgcaacaaca cctatcccgg cgccgcctgc gatgtcccgt
     2941 cgcacttcta ctcctactcg ttcgcgctca acccgcgctg gagcaagacc ttcgccaacc
     3001 aaccggagat cctcgcctac ctcgagaagg tcgccgcaga aaatcgcctg accgaccacc
     3061 tcgaagcacg aacacgggtc cgtaccttgc ggtggtcgga tatcgaccga cgctggacgg
     3121 tgcagaccga cgacggcgcg acgcaccagt tcgacgcggt cgtcaccgca gtcgggatgc
     3181 tcgacgtccc caatatcccg gatatccccg gcgccaagcg ctttcgcggt cgcgccttcc
     3241 attcgtcgaa ctgggatcac acgaaaacca ccgcgggcga acgtgtcgcc tcgatcggaa
     3301 ccggtgccag cgccatccaa tacgttcccg agatcgcgcc ggacaccgcg cagctcacgg
     3361 tttttcaacg gacaccgatc tgggtgagtc cgcgctacga cgaacccttc acacccgaac
     3421 aacatgagct cttcgagcgt gatcccgtcg aggctctcaa ggtgcgtgac gcggcctttg
     3481 aagcctacga gtccgcgaac ttcggcgtcg attcggcgat gaccaaggaa ctgacggaga
     3541 tcgcccacgc gtacttgacg cggaaggtca acgatcccga cttgcgcgcg aagctgactc
     3601 cctcctaccc ggtcggatgc aagcggccgc tgcagtctcg cgcctggttt ccgaccttcg
     3661 cccttccgaa cgtgcgtctg gagacgtcgc ccatcgtcga gttcacagag cgcggcctgc
     3721 gtacggcgaa cggggcagag cacgaggtcg acaccgtcat ctacggcacc ggtttccacg
     3781 ccgccgacta cctccgcagc ctcgacgtct atggcaccga gggccgccga ctacgcgacg
     3841 actggcgaga cggcgccgag gcatatctcg gcacggtcgt ccccggctat cccaacctgt
     3901 tcacgctgta cggccccaac accaacggcg tcacctcgat catctacatc ctggaggcgc
     3961 agaccgagtt cgtccgccgc accctcgacg atatgaccct tcgccgcgta caggcgatcg
     4021 acgtcaagcg gggtgtccac gacgcataca acgacgacat tcaacgggcg atgcaggaca
     4081 ccgtgtggct ggccaactgc aacaactact accgtcatcc caacggcaaa gttgtaaccc
     4141 agttccccta cagcggaacg actttcgtgt cgatgctcga ccaagtagga ctagacgtat
     4201 tcaattggat ggaggcaacc gcatgccagg aatccaccac actgcgatag tcaccgccga
     4261 cgtcgaacga tccgtgcggt tctggtgcga cggcctcggt ctcaccgagc tgatggatta
     4321 cacgttcacc ggcgactggc cgacgctgtt cagtgcgcgc accgaccggc tgcggtcgat
     4381 ctttctcggc gatccggaca cccccgacac gggcatcgtc gaactggttc agttcgacga
     4441 cactggcctt ggcgcaccgc cgcccgacga gcccgccttc ggcttcttcc tgctctcgct
     4501 ccagcgtgac gtcgacgcca ccctggccgc gctggcaggc ctgggcttcg acgacggcgt
     4561 tcggcgtatc gaaatgcccg caccgggtgg caaaacggtt gcgatggcgg tggtcaccgc
     4621 gcccgacggt gtgcgcgtcg aactgatcgg gccgccgcag tgatcgcggt cgtcacggga
     4681 ggcgggtccg ggatcggccg cgctgtcgtc gatcgcctcc gtaacgcggg cgctgatgtc
     4741 gtcgtgtggg atatcaagga cggcgatatc cgttgcgata tcaaggatcc cgagtgggtg
     4801 tcggccgcga tcgaacaaac cgtggctgac cacgggaccc ctgaccggct cgtcgcctgc
     4861 gccggcatcg gggcgtctgg gctgctgctc gagcagaccc ccgccgactg gcagcgcgtc
     4921 atcgacacca acctgaccgg gacgtggctg acgatgagcg ccgttgcccg ggcgatggtc
     4981 gatgcgggct cgggcggatc aatggttgcc gtgtccagca tcagcgggac tctggccgac
     5041 cgcgatatgg gcgcctattg cgtgtcgaag gcgggcatcg gcatgttggt tcgcgtcgcg
     5101 gccgccgagt ggggcgcgca cggtatccgc gtgaacgccg tcggccctgg ggtcacccgc
     5161 acgccgaagc tcgccaagcc cgaacaactg ccagggtggg tcgagggcct taccgaacgc
     5221 accgcactcg gtcggcttgg ggaggccgac gacgtcgccg aggccatcgt cgcggtgctc
     5281 gaaatgtctt gggtcacagg gcagatcatg ttcgccgacg gcggactggc gctacacagc
     5341 cctatcgacg cgtacggaca ggtgcagcgc gtgacgcgag gcggacgcta ggcgcccgtg
     5401 cctgacgggt gcatgccggg cgatgcgctc ggagccatct ggtcgtaaac ccgccaccaa
     5461 gtcatctcgt gggcggttgc cgagatctcg ccccgcgcga gctgctcgtc ggcctccatc
     5521 agtgcgcccg ccagattctc cagcctgtga cggacagcca ctgagaccgg atcgtggtgg
     5581 cctgccatca ccatcgcgcc
//
LOCUS       DASRJA010000795         5180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2956469,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000795 DASRJA010000000
VERSION     DASRJA010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2956469"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VFC01_35850"
     CDS             complement(<1..863)
                     /locus_tag="VFC01_35850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003883462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZN85033.1"
                     /translation="MQRGSPIGPALLVVVAAISQEVGAAFAVGLFAALGATGAVFARL
                     LVAGVILCVAVRPGLRGLSGRAWLSAGALAAAVTVMNVCFYFSLTRIPLGVAVTVEAL
                     GPLALSVIVSRRWTAWLWALLALLGVVLLSLAGVRVGHLDPIGLGFAAGAGASWAGYI
                     LATARAGSDFPKLDGLALATAIGAVGMAPLAVVTIDPQSALRWQVLGLGVVVGVMSSV
                     IPYSLELMSLRRLPPETFAILTCLSPVVAALAGWLILGQHLAVAGYVAIVLVTVASIG
                     AVRSARERLVE"
     gene            887..1318
                     /locus_tag="VFC01_35855"
     CDS             887..1318
                     /locus_tag="VFC01_35855"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013471871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HZN85034.1"
                     /translation="MGAGVAGVCVLVWLAALSVYDIREQRLPNWLTMPGAAVILATAA
                     AQGRGGQAVLGAVALFAVYAVVHLVSPAAMGAGDVKLAIGIGALTGTFGSDVWLLAAF
                     GAPILTAGWAVVALLRESGKTVPHGPSMCLAALTATALAVV"
     gene            complement(1315..2091)
                     /locus_tag="VFC01_35860"
     CDS             complement(1315..2091)
                     /locus_tag="VFC01_35860"
                     /inference="COORDINATES: protein motif:HMM:NF036298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5336 domain-containing protein"
                     /protein_id="HZN85035.1"
                     /translation="MVYSPGTRDYHSAYQPAYPNQRLDAESSLPRNLFIAVAVLGLAT
                     YVFSFGPVADGREAVGWDIRFAVLAALCAMLGLLAKQSPLPLTTAVLATMGFLDGLSS
                     MLAAADRGWALTVIVVLNALQAAAAVAALLLAPKAVPKPADTTGYEAYVDYYNQAVRS
                     YYDQQAQSVPPEQMQRAGYGQASAEARASARAPRSQRPSQHGDYADLDYSGTRTTAPQ
                     QDLAGSASGPSGLPSFGQANTRADQPLREADESTRPSSPA"
     gene            complement(2121..2642)
                     /locus_tag="VFC01_35865"
     CDS             complement(2121..2642)
                     /locus_tag="VFC01_35865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85036.1"
                     /translation="MTASGCGQVIGADEDGRAVALPLFGPQIARVEICGTLHLAQQAV
                     LRSLALGARVLVHSRRPGLWRDMVDEVDDHDLLWVADFNRGAIQAGFDRNYSVEMFDG
                     VPERSVRAGVTSMVVAAPNTAVTPSADVALELVSLADATVKVSTRAGSAMVTMVATDD
                     EMRYIKASANNID"
     gene            2685..3005
                     /locus_tag="VFC01_35870"
     CDS             2685..3005
                     /locus_tag="VFC01_35870"
                     /inference="COORDINATES: protein motif:HMM:NF037656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85037.1"
                     /translation="MSDRPGGFWNRHAAYEELVSACTTLCQGEALDSNGQLRRSIDLA
                     GNGRFVRNVIEAAEEEREYRLSESHGRNLQDLDEAQLMRIELSDMQNALKQVLGMLST
                     NGRK"
     gene            3173..3439
                     /locus_tag="VFC01_35875"
                     /pseudo
     CDS             3173..3439
                     /locus_tag="VFC01_35875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016892974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
     gene            3506..3892
                     /locus_tag="VFC01_35880"
     CDS             3506..3892
                     /locus_tag="VFC01_35880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN85038.1"
                     /translation="MQITQQDLKAISERYFAAWAARDPDAIVALHTEDTRFWTHLGTE
                     AAVGREAVRAAFAEIFEQFPNLTWETYRVLYGEDHWILDWALISGDIRFDCLDVVNVS
                     PDGLVARKDTFVDAMQLQAALGAVNA"
     gene            3889..4380
                     /locus_tag="VFC01_35885"
     CDS             3889..4380
                     /locus_tag="VFC01_35885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HZN85039.1"
                     /translation="MTTAIPVDQLPLVTALPAEHLFDMHVNLQPAQPISTPVGSRMTF
                     ITTGGVIEGPKLRGEVLPGGGDWLIVGSDGVGRVDVRATLRTHDGALIHYETRGVVKI
                     PADGVQRLAAGEVLPFAETYIRTTPKFETSDERYAWLSEVVALGYNILSPNHIDYRMY
                     RVL"
     gene            4446..5045
                     /locus_tag="VFC01_35890"
     CDS             4446..5045
                     /locus_tag="VFC01_35890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN85040.1"
                     /translation="MATKVARRTQAERTEATTGALVDAAREFFARDGYDATSLDAVAG
                     RAGVTKGAVYHHFDGKRQLFEAVFSREVERIALPLATAYGRKKDPWDAFHAGCRAFLD
                     ECLDPGLQRIILLDASAAIGWEKIRQLESPLLEMMELAISRAADAGRIAPRKPGPLAH
                     FLYGALCETAMIVARADDQKAAHRDAVVEIGRVLAGLAL"
     gene            5091..>5180
                     /locus_tag="VFC01_35895"
     CDS             5091..>5180
                     /locus_tag="VFC01_35895"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZN85041.1"
                     /translation="MLRWTTAGESHGRALVAVVEGMVAGVHLTS"
BASE COUNT          902 a         1733 c         1719 g          826 t
ORIGIN      
        1 ctctctacca agcgttcccg tgccgagcgc acggccccga tgctggccac ggtaaccagc
       61 acgatcgcca catagcccgc cactgcgagg tgctgcccaa ggatcagcca gcccgccagg
      121 gcggccacga ccggtgaaag acatgtgagg atcgcgaacg tctccggcgg gaggcggcgc
      181 agcgacatca actccagcga gtaggggatc accgacgaca tcactccgac aactaccccc
      241 agccccaata cctgccaccg cagcgcgctc tgcggatcga tcgtgaccac cgctaacggt
      301 gccatcccca ctgcaccgat cgccgtcgca agggccagcc cgtcgagctt ggggaaatcg
      361 gatcccgcgc gtgccgtggc gaggatgtaa cccgcccagc tcgcccccgc gcctgcggcg
      421 aaccccagtc caatcgggtc gagatggccc actcgaaccc ctgcgaggct gagcagcacg
      481 acgcccagca acgcgagcag agcccacagc catgcagtcc agcgtcggct gactatcacg
      541 gaaagtgcca ggggaccaag ggcttcaacg gtgacagcaa cgccgagagg gatgcgggtc
      601 aacgagaaat agaagcacac gttcatcacg gtcaccgcgg cggcaagcgc acccgctgac
      661 aaccacgccc ggccggacag tccccgcaac cccggccgaa ccgcgacgca caggatcacc
      721 cccgcgacaa ggagccgggc gaagacggcc cccgtcgcac cgagcgcggc gaacaggccg
      781 accgcgaacg cggcgccgac ctcttggctg atcgcggcga ccaccaccag gagcgccggt
      841 ccaattggtg accctcgctg caccgcaccg aggttaatct ccatacatgg gggcgggggt
      901 ggccggtgtc tgcgtgttgg tgtggcttgc cgcgctgagc gtctatgaca tccgtgagca
      961 acggctgccg aactggctga cgatgcccgg cgccgcggtg atcctcgcga cggcggcggc
     1021 gcagggccgt ggcggtcaag ccgtgctcgg cgctgtcgcg ttgttcgcgg tctatgccgt
     1081 cgtacatctt gtgtctcccg cggcgatggg cgccggcgat gtgaagctgg cgatcggcat
     1141 cggtgcgctc acagggacgt tcggcagtga cgtctggctg ctggcagcat tcggcgcacc
     1201 gatactcacc gcggggtggg ccgtcgtcgc gctgctgcgc gagtcgggaa agactgtgcc
     1261 gcacggtccg tcgatgtgtc tcgcggccct gaccgccacc gcactggctg tggtttaggc
     1321 cggtgacgac gggcgcgtgg actcgtcggc ctcgcggagg ggttggtccg cccgcgtgtt
     1381 cgcctggccg aagctgggca gccccgacgg ccctgacgcc gagccggcaa ggtcctgctg
     1441 tggtgcggta gtgcgagtac ccgagtagtc cagatcggcg taatcgccgt gctgagaagg
     1501 tcgttggctg cgcggcgccc gcgcgctggc ccgcgcctcg gcggacgcct ggccgtaccc
     1561 ggcgcgttgc atctgctcgg gcggcaccga ctgtgcttgc tggtcgtagt aactccggac
     1621 ggcctggttg taatagtcga cgtaggcctc gtagccggtc gtgtcggcgg gtttggggac
     1681 agctttgggc gccaacagca gcgcggccac cgcggccgcc gcttgcaggg cgttgagcac
     1741 cacgatcacg gtcaacgccc atcctcggtc ggcggccgcc agcatgctcg acagcccgtc
     1801 gagaaagccc atcgtcgcca agactgcggt cgtcagcggc agggggctct gtttggccag
     1861 caatccgagc atcgcgcaca gtgcggccag tacggcgaac cggatgtccc atccgaccgc
     1921 ctcccggcca tcggcaaccg gaccgaagct gaatacgtag gtggccagcc ccagtaccgc
     1981 tactgcgatg aaaaggtttc tcggcagcga actttcggca tcgagccgct ggtttggata
     2041 cgcaggctga taggccgagt ggtagtcgcg ggtcccaggc gaatacacca cgatcctcac
     2101 gctagcgcac tgcgcggcga tcagtcgatg ttgttggccg acgccttgat gtagcgcatt
     2161 tcgtcgtcgg tggcgaccat cgtgaccatt gcggacccgg cccgcgtgct caccttaact
     2221 gtggcgtccg ctagcgaaac caactcgagc gcaacgtcgg cgctgggcgt caccgcggta
     2281 ttcggcgcag ctacaaccat gctcgtgact ccggcacgca ccgaacgctc cggtaccccg
     2341 tcgaacatct ccaccgaata gttgcggtca aagccggctt ggatcgcgcc gcggttgaag
     2401 tcggcgaccc acagcaggtc gtggtcgtcg acctcgtcca ccatgtcgcg ccacagcccg
     2461 ggacgtctgc tgtgcacgag cacccgtgcg ccgagcgcca gcgagcgcag caccgcctgc
     2521 tgggccaggt gcaaggtgcc gcagatctcg acccgggcga tctgcgggcc gaacagcggt
     2581 agtgcgacgg cccgcccgtc ctcgtcggcg ccgatcacct ggccgcatcc ggacgcagtc
     2641 accgcgagtt cgctcaggtc gatgtcgcgt cgaccgtagg ccgagtgtcc gaccggccgg
     2701 gtgggttctg gaatcggcac gccgcctatg aggaacttgt ctcggcgtgc accaccctat
     2761 gccaagggga ggcgctcgac tcgaatggcc agctgcggcg cagcatcgat ctggccggca
     2821 atgggcggtt cgtgcgcaac gtgatcgagg cggccgagga ggaacgcgag tatcgcctgt
     2881 cggagagtca cggccggaat ctgcaggatc tcgatgaagc ccaattgatg cgcatcgaac
     2941 tttctgacat gcagaacgcg ctcaagcagg tgctcggcat gctgagcacg aacggacgga
     3001 agtgaccatg acgcaaccgt tctctgacag cgacaccgcg ccactgcgcc tgcccgtgac
     3061 tccctcgcca ccgcccgcac ccgagggtgc ggcgaatggg gcacgaacac cggatccggc
     3121 atcgctacat ccgcctgagg caacgccatt caccagtgcc gcgtcgaaca gaccgcgcgc
     3181 acaagcgccg acaaacagcg aactcggcct tgttcggagc cggccggcac cgaaatccgg
     3241 ctggcgcaaa gcgattcatc gcgccacggg tttgaatccc ggcgagtctg cggctgagac
     3301 gactcacagg gcgatagtcg accgcgtcaa tcagccggtg cgtggcgggc cgttcaccat
     3361 cgcagtgctg tcccagaagg gcggtgttgg caagacgacc acgacggtgg gattgggcgc
     3421 cacattggcc gcgatccagc ccacagtcgg gaggtctgtc ggagcctact tgcgtactta
     3481 gggtaggtta gtacatactg cgggtatgca aatcacccag caggatctaa aagccatttc
     3541 ggagcgctac ttcgccgcct gggcggcgcg cgaccccgac gcgatcgtcg cgctgcacac
     3601 cgaggacacc cggttctgga cgcacctcgg taccgaagct gcagtcggcc gcgaggccgt
     3661 gcgtgccgcg ttcgccgaga tcttcgagca gttccccaac ctcacgtggg agacctaccg
     3721 ggtgctctac ggcgaggatc attggatcct cgactgggcg ttgatttccg gcgacatccg
     3781 cttcgactgc ctcgatgtgg tcaacgtctc gccggacggg ctggtggccc gcaaggacac
     3841 gttcgtcgac gccatgcagc tgcaggcggc gttgggcgcg gtgaacgcat gactaccgca
     3901 ataccagtgg accagctgcc gctcgtcacc gcgctgcccg ccgagcatct gttcgacatg
     3961 cacgtgaatc tgcaaccggc gcaaccgatc tcgacacccg tcggctcccg gatgacattc
     4021 atcacgaccg gcggtgtgat cgaagggcca aagctgcgcg gcgaggtgct gcccggtggg
     4081 ggagactggc tcatcgtcgg cagtgacggt gtggggcgcg tcgacgttcg cgccaccctg
     4141 cgcacacacg acggtgctct gatccactac gaaacccgcg gcgtcgtcaa gattccggcc
     4201 gacggtgtgc aacgactggc cgcgggcgag gtgctcccat tcgcggagac ctacatccgc
     4261 accactccga agttcgagac ctcggatgag cggtacgcgt ggctgtccga ggtggttgcg
     4321 ctcggctata acatcctgtc gcctaaccac atcgattacc ggatgtatcg ggtgctttga
     4381 ggtggctcga ccgcgctgat cgcgttggtg ccggaacata cgtgaacacc ggctatggtc
     4441 aggccgtggc gacgaaggtg gcgagacgga cccaggccga gcggaccgag gcgaccaccg
     4501 gcgcgctcgt cgacgcggcg cgcgagttct ttgcccgcga cggctacgac gccacctcgc
     4561 tcgacgcggt ggcgggcagg gcaggcgtca cgaagggtgc cgtctaccac cacttcgacg
     4621 gtaagcggca actgttcgag gccgtattca gccgcgaggt cgaacgaata gcactgccgc
     4681 tcgcgactgc gtacggacgc aagaaggatc cgtgggacgc cttccacgcg ggatgccggg
     4741 ctttcttgga tgagtgtctt gatcctgggc tgcagcggat catcctgctc gacgcatccg
     4801 ccgcgatcgg ctgggagaag atccgtcagc tcgaatctcc cttgctcgag atgatggagc
     4861 tcgcgatctc ccgcgcggcc gacgccggcc ggatcgcgcc gcgcaagccg ggcccgctgg
     4921 cccacttctt atatggcgcg ctgtgtgaga ccgcgatgat cgtcgcccgc gccgacgacc
     4981 agaaggccgc ccaccgtgac gcagtcgtcg agatcggccg cgtcctggcc ggcctggccc
     5041 tctagaaagc gatttcggct ggacaagcgc gtcatgggaa gatgggacgc gtgttgcgat
     5101 ggaccactgc tggtgaatcc cacggccgcg cgttggtggc cgtggtcgag ggcatggttg
     5161 caggggtcca cctcacctcc
//
LOCUS       DASRJA010000796         7111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2674513,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000796 DASRJA010000000
VERSION     DASRJA010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2674513"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..895
                     /locus_tag="VFC01_35900"
     CDS             44..895
                     /locus_tag="VFC01_35900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HZN85042.1"
                     /translation="MGAITLDGKATRDEIFVDLKERVEALTAAGHTPGLGTVLVGDDP
                     GSHAYVRGKHADCAKVGINSIRRDLPADISQAKLDETIDELNANPECTGYIVQLPLPK
                     HLNENAALERVDPGKDADGLHPTNLGRLVLNEPAPLPCTPRGIVHLLRRYEVEIAGAQ
                     VVVIGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDLAALTRQADIIIAAAGVQHLVT
                     ADMVRPGAAVVDVGVARDAEGKLVGDVHPDVWEVAGHVSPNPGGVGPLTRAFLLTNVV
                     ERAEALA"
     gene            892..1185
                     /locus_tag="VFC01_35905"
     CDS             892..1185
                     /locus_tag="VFC01_35905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3017 domain-containing protein"
                     /protein_id="HZN85043.1"
                     /translation="MTLKDFARKVFGGQWPILLVGVIIVAAFGLVVAGYWRRGGLVLA
                     IGVGVAAAFRLALPEDRVGLLAVRSRTIDFATTATVSAAMLYIAWTIDPLGTS"
     gene            complement(1214..1789)
                     /locus_tag="VFC01_35910"
     CDS             complement(1214..1789)
                     /locus_tag="VFC01_35910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZN85044.1"
                     /translation="MTAIYTFDVFSSLDGYGGVSGGDWGGYWGKQGPELLDHRLALYE
                     AEQRMVFGANTYRAFVEILASGAEEVRDPWVTRMRHLPATVVSTTLDGPLDWPDATVV
                     SGDAVDVVARLKQESEVPLRSHGSLSMNRALMAAGLVDRVQVTFFPVITGQTGDDPIF
                     QGAADYDLELIESRTLDGHIQELIYRPTLHI"
     gene            complement(1866..2489)
                     /locus_tag="VFC01_35915"
     CDS             complement(1866..2489)
                     /locus_tag="VFC01_35915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN85045.1"
                     /translation="MPRLYVLAGVNGAGKSSIGGAMIRAAGADYFNPDEAARRLIAAN
                     PGLDQVKANATAWHQGRRLLERAIRERKDFAFETTLGGNTMPRLLAEAASQGFEVRVW
                     YVGLASPDLHIERVRNRVRAGGHDIPESSIRRRWRHSRLNLVQSLPHLTELRVYDNSA
                     DADPAAGNAPQPVLVLHLDHGEIVGPQDLSSTPDWAKPIVAAALKLG"
     gene            complement(2490..2846)
                     /locus_tag="VFC01_35920"
     CDS             complement(2490..2846)
                     /locus_tag="VFC01_35920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HZN85046.1"
                     /translation="MATLTFRNRDGELVDVPTVAATRFKNEFGSIFEQAALGGAVAIT
                     KHNTPKAVLLSYAEFEALTKASMPALDDLSDEFDLLLETMQTPHSKDAMASAFDATPE
                     HLGRAAVKAARPKRRR"
     gene            2944..3546
                     /locus_tag="VFC01_35925"
     CDS             2944..3546
                     /locus_tag="VFC01_35925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN85047.1"
                     /translation="MTAPLGLRERKKLGTRWALSDAALELALERGLENVTREDIANRA
                     GVSLRTFNNYFTGKYEAVAFRQIDRTRQSLAVFRERPADEPLWSAITEAMLAPLEAES
                     ATDMRPTPEELTVIRELLSARDLRAALTRDLFADWVDAIAERTGTDPARDMYPRLVTA
                     VVRAVGETAMEAYAYADPPVPYTELLRRGFADVAAGLPER"
     gene            3552..5138
                     /locus_tag="VFC01_35930"
     CDS             3552..5138
                     /locus_tag="VFC01_35930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZN85048.1"
                     /translation="MSETQVPVVISGAGPNGLMLACELALAGVRPIVLERLPAPSDEP
                     KANGMIGQVIRLLDMRGLYRRFSGSDEPPKPVYEWIFSGMSVPLFGVRDNPMYALMMS
                     QPRLVRSLDERARELGVDVRWGHEFIGVESRLDGVKVSVSTPDETYDLDATYLVGADG
                     GRSTVRKSAGIDFPGHTSNTVARVAHVHVPDEMLTANGDLDVPGVGRLPFGHNRLDRG
                     GLIYAPFERDRPMVGTIEYDQQADDAEPMSIDELRDSARRVLGVDLPVEPPAGSGPHA
                     LRRIAGQNTRQADRYRAENIFLLGDSAHVHSAMGGPGLNLGLQDAMNLGWKLAAAVNG
                     WAPSDLLDTYQSERYPVGERVMMHSMSQTALFGPGPEIGALRKLFTELLQLPSVAEHM
                     AHLLAGSDVRYEVGDDHPLAGRLVPDVTLGDGRRVAELLHHGRPVLLDLCGRVEDAAR
                     GWTDRVDVVSGAVPDLEVCVLLIRPDGYVAWAADTFEADDEKRLHTALRKWFGVSAAE
                     RGAARRDDGLSSAPAAERTA"
     gene            complement(5062..5481)
                     /locus_tag="VFC01_35935"
     CDS             complement(5062..5481)
                     /locus_tag="VFC01_35935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZN85049.1"
                     /translation="MTETKDAVAVFCEATRARDIDGIVSALAPDAELLSPLSGRMVFR
                     GRDDLRVLLGAVYGGLSNLTWREVIGEGTTRVAVSDARIAGVTITDAMVLELNDDGLI
                     VRIRPHLRPWLAITLFALLLGPKIARHPGVLRRALRR"
     gene            5541..6047
                     /locus_tag="VFC01_35940"
     CDS             5541..6047
                     /locus_tag="VFC01_35940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN85050.1"
                     /translation="MPKQDFADISCSIARTVGVVGERWTPLIVRDLFAGMTKFEDIRR
                     DLGIASNILAARLDDLESNGVVKRRQYQSTPARHEYLLTTMGRDLYPVIATLLAFGDK
                     WLSEPDGPPALTVHTDCGQVTTAKTVCAECGGELNADNAIHTAGPGAKPGPGTALIGD
                     YITGRNSF"
     gene            complement(6039..>7111)
                     /locus_tag="VFC01_35945"
     CDS             complement(6039..>7111)
                     /locus_tag="VFC01_35945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZN85051.1"
                     /translation="SIAQTGYTVATRCFDAPHMRFNVADAHLSALLLTGRISEAIDVA
                     ERVRQQAADLPGAAESLGSAVAGRAALGAGNLETSCALLDQAAAALSASGHTIGWGYR
                     YHVPYTTALAIRGSTDEAAEVLGALGKLRRPFRSLDYERSLAQAWVSASQGAINEAIT
                     ILQSAADTAAANGRFAAEVLCLQTATQFGFRSGAPRLRELEKLVDGPRVGLAARFADA
                     LGVDDPAELAALSKDFEAMGDLVAAVDAAAHAAMAYRRRDLRGSALSSSGRAEELAAL
                     CGGADTPALRQAREPLPLTDREREIVMLVGGGMSNKDIAVRLSLSVRTVEGHIYKAMN
                     KTGTANREELAALLPKHMPESE"
BASE COUNT         1225 a         2336 c         2363 g         1187 t
ORIGIN      
        1 cagactgggc gcgcaggcga gcgaagcgac gggagaaaac tccgtgggtg cgatcacatt
       61 ggacggtaag gccacacgag acgaaatctt cgtcgacctc aaggagcggg tggaggcgct
      121 gaccgcagcc ggccacacac caggactggg caccgtgctg gtcggcgacg acccgggctc
      181 acatgcctac gtccggggca agcacgcgga ctgcgccaag gtgggcatca actcgatccg
      241 ccgcgatctg cccgccgaca tcagccaagc caagctcgac gagaccatcg acgagctcaa
      301 cgccaacccc gagtgcaccg gctacatcgt gcagctgccg ctgccgaagc acttaaacga
      361 gaacgccgca ctggagcgcg tcgaccccgg caaggacgcc gacgggctgc atccgaccaa
      421 cctcggcagg ctggtgctca acgagcccgc gccgctgccg tgcacgcccc gcggcatcgt
      481 gcacctgttg cgccgctacg aggtggagat cgccggcgcc caggttgtgg tgatcggccg
      541 tggtgtcacc gtcggcagac cgcttgggct actgctcact cggcgctcgg agaatgcgac
      601 ggtgacgttg tgccacaccg ggactcgtga cttggcagcg ctgacccgcc aagccgacat
      661 catcatcgcc gcagcgggcg tacagcacct ggtgaccgcc gacatggtgc gtccgggtgc
      721 ggctgtggtg gacgtgggcg tggcgcgtga cgcggaaggg aagctggtcg gtgacgtgca
      781 ccccgacgtg tgggaggtgg ccggccacgt ctcgccgaac cccggtggtg tcggcccgct
      841 gacgcgggcg ttcctgctga cgaacgtcgt cgagcgggca gaagccctcg cgtgacgcta
      901 aaggacttcg cccgcaaggt gtttggcggg cagtggccca tcctgctggt cggcgtgatc
      961 attgtggcgg cattcggatt ggtggtcgcc ggctattggc gacgcggtgg actggtgctg
     1021 gccatcgggg tcggcgtggc ggcggcgttt cggttggcgt tgcccgagga ccgggtcggg
     1081 ctgctggcgg tgcgcagcag gaccatcgac ttcgccacca ccgcgacggt cagcgcggcg
     1141 atgctctaca tcgcgtggac cattgatccg ctgggcacca gctagcgagt actcggcgaa
     1201 gagcggggcc gactcagata tgcagggtgg gccggtagat gagctcttgg atgtggccgt
     1261 cgagcgtccg gctctcgatc agctcgaggt cgtagtcggc cgcaccctgg aagatcgggt
     1321 catcaccggt ctgaccggtg atcacaggaa agaacgtcac ctggacgcga tcgaccaggc
     1381 cggcggccat cagcgcccgg ttcatcgaca ggctgccgtg cgagcgcaac ggcacctcgg
     1441 actcctgctt gagccgggcg acgacgtcga cggcgtcgcc gctcacgacg gttgcgtcag
     1501 gccagtcgag aggtccatcc agagtcgtcg acacgaccgt cgccggcagg tgcctcatcc
     1561 gggtgaccca tgggtcacgc acctcctcgg cgcccgaggc cagaatctcc acgaacgccc
     1621 gatacgtgtt agctccgaag accatccgct gctccgcctc gtacaaggcg agacggtgat
     1681 cgagcagctc ggggccttgc ttgccccagt agccgcccca gtcgccgccg ctgacgccgc
     1741 cgtagccgtc gaggctggaa aagacgtcga aggtgtagat agcggtcatg atggtctcct
     1801 cgagtgccgt tgatcggttc ttgggaagac agacctggaa ccacggcgag attcatcgcc
     1861 gttggtcacc ccaatttcag ggctgccgcg acgatcggct tcgcccagtc aggcgtcgac
     1921 gaaaggtcct gcgggccaac gatttcgccg tggtcgaggt gcaacaccag caccggctgc
     1981 ggtgcgttcc cggccgctgg gtcggcgtcc gctgagttgt cgtagacacg gagctcggtc
     2041 aggtgtggca gcgactgcac caggttcaac ctgctgtgcc gccagcggcg ccggatgctg
     2101 gactctggaa tgtcatggcc gcctgcccgc acgcggttgc ggacccgttc gatgtgcaga
     2161 tccgggctgg ccagtccgac gtaccagacg cgcacctcga agccctgcga cgccgcctcg
     2221 gcgagcagtc gcggcatggt gttcccgccg agcgtggttt cgaacgcgaa gtccttgcgt
     2281 tcccggatcg cccgctcgag cagccgtctg ccctgatgcc acgccgtggc attggccttc
     2341 acctggtcga gtcccgggtt ggccgcgatc aacctgcgtg ccgcctcgtc ggggttgaaa
     2401 tagtcggcgc cggcagcgcg gatcatggcg ccgccgatgc tgctcttgcc tgcgccgttg
     2461 actccggcga ggacatagag gcgtggcagt tagcgccgcc gtttcggacg ggccgccttc
     2521 accgcggcgc ggccgaggtg ttccggcgtg gcgtcgaacg ccgacgccat ggcgtccttg
     2581 gaatgcggtg tctgcatggt ctccagcagt aggtcgaatt cgtcggacag gtcgtcgagc
     2641 gcgggcatgc tcgccttggt cagtgcttca aactcggcat aggacaacaa caccgccttg
     2701 ggcgtgttgt gcttggtgat cgcgacagcg ccgccgagag cggcctgttc gaagatgctg
     2761 ccgaactcgt tcttgaagcg ggtggcggcg acggtcggga cgtcgacgag ctcgccgtcg
     2821 cggttgcgga acgtcagtgt ggccataaca gccattctag ccattatgtc taaatcggcc
     2881 accgataacc ttgcctgata ggcaatcttg cccggcgtgc aagtttagtc taccttggtt
     2941 tctatgaccg caccgctggg actgcgtgag cgcaagaagc tcgggacccg ctgggcgctc
     3001 agcgatgccg cgttggagct cgccttggag cgagggcttg agaacgtcac ccgcgaagac
     3061 atcgccaaca gggccggggt atcgctgcgg accttcaaca actacttcac gggcaagtac
     3121 gaggcggttg ccttccggca gatcgatcga acgcgacaga gcctcgcggt cttccgtgag
     3181 cgaccggccg acgagccgct gtggtcggcg atcacggaag caatgcttgc gccgctggag
     3241 gccgagagcg ccacggacat gcggccgacg cccgaggagc tcaccgtcat ccgggagcta
     3301 ctgtccgccc gggacctgcg tgccgcgctg accagggacc tcttcgccga ctgggtcgac
     3361 gcgatcgcag aacgcaccgg gaccgatccg gctcgcgata tgtacccacg attggtcact
     3421 gcggtcgtcc gggcggtcgg tgaaaccgcg atggaggcct atgcctacgc cgatccaccc
     3481 gtgccctata ccgaactcct tcgtcgcggc tttgccgatg tcgcggccgg attgcccgaa
     3541 aggtgatgcc gatgtccgaa acccaagttc ccgtagtcat ctcaggagca ggccccaacg
     3601 ggctgatgct cgcgtgcgag ctcgcgctcg ccggcgtccg gcccatcgtg ctggaacggc
     3661 tgcccgcacc gagcgacgag ccgaaagcca acggcatgat cggccaggtc attcggcttc
     3721 tggacatgcg ggggctttac cgacgcttca gcggctcgga cgaaccacca aagcccgtgt
     3781 acgagtggat cttcagcggc atgtcagtgc cactgttcgg cgtgcgcgac aacccgatgt
     3841 acgcactgat gatgtcccag ccgcggctgg tccgatcgct tgacgagcgg gcccgcgagc
     3901 tcggcgtcga cgttcggtgg ggccacgagt ttatcggcgt ggaatcccgt ttggacggtg
     3961 tgaaagtcag cgtctcgacg ccggatgaaa cctacgactt ggacgcgacc tacctcgtcg
     4021 gagccgacgg cgggcgcagc accgtgcgca agtcggccgg catcgacttt cctggccaca
     4081 catcgaacac ggtggcccgc gtcgcccacg tgcatgtgcc cgacgaaatg ctcaccgcca
     4141 atggcgatct cgatgtgcca ggcgtgggac ggctgccgtt cggccacaac cggctcgacc
     4201 gcggtgggct catctatgcc ccgttcgaac gcgatcgccc catggtcggc accatcgaat
     4261 acgaccagca ggcggacgat gccgaaccga tgagcatcga cgaactgcgt gacagcgcgc
     4321 ggcgagtgct cggtgtcgat ctcccggtcg aaccgcccgc tggcagcggg ccgcatgcct
     4381 tgagacgcat tgccgggcag aacacgcgcc aggccgaccg ctaccgcgcg gaaaacattt
     4441 tcctgcttgg ggattccgcg catgtgcact ctgcaatggg tggtcctggc ctcaacctgg
     4501 gcctgcagga tgcgatgaac ctcggctgga aactggccgc cgctgtcaac ggctgggcgc
     4561 cctccgatct gctggacacc tatcaaagcg aacggtatcc cgtcggcgag cgcgtgatga
     4621 tgcactccat gtcccagacc gcgcttttcg gtcccggacc cgagatcggc gcgctgcgaa
     4681 agcttttcac ggagctgctg cagctaccga gcgtcgctga acacatggcg cacctgctcg
     4741 ccggctccga cgtccgctac gaggtgggtg acgaccaccc cctggcgggg cggttggtgc
     4801 cggacgtgac cctcggcgat gggcggcggg tggccgaact gctgcaccac ggccgcccag
     4861 tactgctcga cctgtgcgga cgtgtggagg acgccgcacg cggatggacc gaccgcgtcg
     4921 atgtcgtgtc cggcgccgta cccgatctcg aggtctgcgt gctgctgata cggccggacg
     4981 gctacgtcgc ttgggcggca gacactttcg aagccgacga cgagaaacgg ctgcacactg
     5041 cgctgcggaa gtggttcggt gtcagcgccg cagagcgcgg cgcagcacgc cgggatgacg
     5101 ggctatcttc ggccccagca gcagagcgaa cagcgtgatg gccagccacg gccgcaggtg
     5161 aggccggatc cgcacaatca gaccgtcgtc gttgagttcg agcaccatcg cgtcggttat
     5221 cgtgacgccg gcgatccgcg cgtcgctgac cgccacgcgg gtggtgccct cgccgatgac
     5281 ctcgcgccat gtcaggttgg acagcccgcc gtagaccgcg ccaagcagca cccgcaggtc
     5341 gtcgcggccg cggaacacca tgcggccgga tagcggcgac aacagctccg catcaggcgc
     5401 gagtgcgctc acgattccgt cgatatcgcg ggcgcgggtc gcctcgcaga acaccgcaac
     5461 cgcgtccttc gtctcagtca tatccataca acccaactta tccagctcca ttgcaaaaaa
     5521 caatggacta ttctgacggc atgccgaagc aggatttcgc agacatctcg tgctcgatcg
     5581 cgcgcacggt cggtgtagtc ggggagcggt ggacgccgtt gatcgtgcgg gatctgttcg
     5641 cgggtatgac gaagttcgag gacatccgcc gcgatctcgg tatcgcgtcc aacatcctgg
     5701 ccgcccggct ggacgatctc gaaagcaacg gcgtcgtgaa acggcgtcag taccagagca
     5761 cgccggcgcg ccacgaatat ctgctgacca cgatgggtcg ggacctgtac ccggtgattg
     5821 cgacgctgct cgctttcggc gacaaatggc tatccgagcc cgacggcccg ccggcgctga
     5881 ccgtgcacac tgactgcggc caggtcacga ccgccaagac ggtgtgcgcc gaatgcggcg
     5941 gagaactcaa cgccgacaat gcgatccaca ctgctgggcc cggtgccaag ccggggccgg
     6001 gaaccgccct catcggcgac tacatcaccg gccgtaactc attctgactc cggcatatgt
     6061 ttcggcagca gcgccgcgag ttcctcgcgg ttggccgtac cggtcttgtt catcgccttg
     6121 tagatgtggc cttcgaccgt tcgaaccgac aggctcagtc gaacggcaat gtccttgttg
     6181 gacattcccc cgccgaccag catcacgatc tcgcgttccc gatcggtcag cggcaggggc
     6241 tcgcgcgcct gacgaagtgc cggagtatcg gcgccaccgc atagtgcggc aagctcttct
     6301 gcccggccgc tggatgacaa cgccgatcca cgcagatccc gacggcgata cgccatcgcc
     6361 gcatgtgcgg ccgcatccac cgccgccacc agatcgccca tcgcctcgaa atcctttgac
     6421 aacgcggcta attcggctgg atcgtcaact cccagcgcgt cggcgaagcg tgccgccagg
     6481 ccgacacgag ggccgtcaac caacttttcg agttctcgca gacgaggagc gccggagcga
     6541 aatccgaact gtgtggccgt ctgaaggcag agcacttcgg ccgcgaatcg gccattggct
     6601 gcagcggtat cggcggcgga ctgcaagatc gtgatcgcct cattgatggc gccctggcta
     6661 gcggacaccc atgcctgggc gaggctccgc tcatagtcga gggaccgaaa tgggcgtcgc
     6721 agcttgccca gcgcgccaag cacttccgcg gcctcatcag tggagccgcg aatggcaagc
     6781 gcggtcgtgt acgggacgtg atagcggtac ccccagccga tggtgtggcc cgacgcggac
     6841 aacgctgctg cggcctgatc cagcaacgcg caggaggtct cgaggttgcc ggcgccgagc
     6901 gccgcccgac ctgcaacggc agaaccgagc gattcagccg ctccagggag gtctgctgcc
     6961 tgctgacgca cccgctcggc cacatcgatc gcttcgctga tgcggccagt gagcaagagc
     7021 gcgctcaggt gcgcatcggc gacgttgaac ctcatgtgcg gcgcgtcgaa acaccgcgtg
     7081 gcgacggtgt atcccgtctg ggcgatggaa a
//
LOCUS       DASRJA010000797        13562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3236488,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000797 DASRJA010000000
VERSION     DASRJA010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 13562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13562
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3236488"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFC01_35950"
     CDS             <1..318
                     /locus_tag="VFC01_35950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HZN85052.1"
                     /translation="VWTWPDRPGVAGCAALDGTVLVNLVNTKQTVAVRLAPDTGAVTG
                     DPEVVRQDQHGHAWALQLSPDGNVWGATVNKTSGDAEKLDDVVFPLFPQGGGFPRSNA
                     DNT"
     gene            complement(331..648)
                     /locus_tag="VFC01_35955"
     CDS             complement(331..648)
                     /locus_tag="VFC01_35955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HZN85053.1"
                     /translation="MPWLVLICAGLLEIVWATALKQSDGFSRLWPSVIGVGGAVLSFV
                     LLAIALRHLPAGTGYAVWVGIGAVGVVIAGVVVFGEAASAARLLFLSIIVVGIVGLRL
                     VEG"
     gene            complement(654..1394)
                     /locus_tag="VFC01_35960"
     CDS             complement(654..1394)
                     /locus_tag="VFC01_35960"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011210300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HZN85054.1"
                     /translation="MTELAGKVALVTGGSRGIGAAIAARLAELGSDVAITYAQSKERA
                     DGVVDTIKAHGRRGLAIKADSADANEVQAAVGQAADEFGRLDVLVNNAGIFPFGPIDD
                     TTLEEVDRALGIHARAVFLASQAAVRHMTAGGRIISIGSNLAERVPFSGVTLYSMSKS
                     ALIGFTRGLARDLGERGITVNLAQPGSTDTDMNPADGPTADGQRALTALGHYANPTDI
                     ANTVAFLAGDGARNITGSVLTVDGGTNA"
     gene            1494..2102
                     /locus_tag="VFC01_35965"
     CDS             1494..2102
                     /locus_tag="VFC01_35965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN85055.1"
                     /translation="MAARGRPRAFDRNGALRKAMKVFWEHGYEAASISDLTAAMGINS
                     PSLYAAFGCKEALFREAVDLYNETEGAAVAAALRDLPTAREAITEVMRHQVMAYTDPE
                     KPRGCMIVLTATTYTERSHAVHEHLADERLATEHDFRERIERGIADGDVPAGANAATI
                     AAFYNAVNQGMAIQARDGADRSKLSTVAESAISAWDGLVGAG"
     gene            complement(2103..3011)
                     /locus_tag="VFC01_35970"
     CDS             complement(2103..3011)
                     /locus_tag="VFC01_35970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZN85056.1"
                     /translation="MIESIRRDDYGFFGPDSPSWKVWASPTALIGFQRAVVLEHFDPF
                     LAAAVADSAGIYTDPSGRLDRTLAYFLTVAVSDGRTAIEVSDALMRIHARAKGVEPIT
                     GSRYSANNPDAQLWIHITGWHSVLKCYEQYGPGPLSRAEESRFWAESRIAAELQTCKP
                     ADVPASRDDVRQYFTDVRGRLCTSEHADRAMHYLLRTPRDRGVRLWAGSRLLAPAAIA
                     TLPKWMRHMGNFNQPAAVDAAVTPVARAWVKALSANNFRPMLAVARQIAPETAEVLAQ
                     HLSAGPPERRTTTTPAEARERLAIHA"
     gene            3097..3717
                     /locus_tag="VFC01_35975"
     CDS             3097..3717
                     /locus_tag="VFC01_35975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN85057.1"
                     /translation="MIGPRPRRKYAKRLPREQRREQLLDAAMTVLGGCQLHELSMEAV
                     AEAAGVGKPVLYTVFRTRAELVSALLRREHQRGLEQVLAGMPDDLTAPGPTGAYAATV
                     SAFLRVVLENPMRWRLILTVPDSAPREYRDDLRRARSSILAQAENLAKAGIALEPRLA
                     DLDPALLGHTMLSFAEMLGRLAINDPETYPRERLEEFAAAAMGIRR"
     gene            complement(3714..5216)
                     /gene="gatB"
                     /locus_tag="VFC01_35980"
     CDS             complement(3714..5216)
                     /gene="gatB"
                     /locus_tag="VFC01_35980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZN85058.1"
                     /translation="MTVAAAELVDYDDVVARYEPVLGLEVHVELSTETKMFCGCANKF
                     GAEPNTQVCPVCLGLPGSLPVLNQTAVESAIRIGLALGCEIVPWCRFARKNYFYPDMP
                     KNYQISQYDEPIAINGYLDVPLDDGTTWRVDIERAHMEEDTGKLTHLGSETGRIEGAT
                     TSLIDYNRSGVPLIEIVTKPIEGTGERAPEIARAYVTALRDLLRGLGVSDVRMDQGSM
                     RCDSNVSLKPTGQKEFGTRTETKNVNSLKSVEVAVRYEMRRQAAVLDSGGRVVQETRH
                     FHEDGYTSPGRSKETAQDYRYFPEPDLEPVAPDAEVVERLRQTIPELPWLQRKRIQQE
                     WGISDEVMRDLVNIGAIDLITATVQQGASSESARAWWGNFLVQKANEAGVELDALPIT
                     PAQVAAVVKLVDEGKLSNKLARQVVEGVLAGEGEPEQVMAARGLEVVRDDSALQAAVD
                     EALAANPDVVEKIRGGKVAAAGAIVGAVMKATKGQADAARVRELVLAACS"
     gene            complement(5241..5714)
                     /locus_tag="VFC01_35985"
     CDS             complement(5241..5714)
                     /locus_tag="VFC01_35985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZN85059.1"
                     /translation="MPDREAGIAGLHQRIPFLLSQLGIYLAEDFVHRLEPFGVEPRAY
                     AVMKALSEDDGRSQRELSTQLGIHRNVMVTVVDKLEREGLVKRMPHPADRRAFAVTLT
                     DKARELLPKLDAEGRTQEDEITALLTDDERKAVLQHLQRMSTAAGLIPGVHPKLG"
     gene            5789..6487
                     /locus_tag="VFC01_35990"
     CDS             5789..6487
                     /locus_tag="VFC01_35990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HZN85060.1"
                     /translation="MDIVVFGANGQTGRLLTRRALDKGHSVVAVTRHPDDFPLSHSLL
                     TVARADVREPSSLVDVVRGADAVVSTLGVPFTTRSIDTYSVGVRNIVDAMQMTGVQRL
                     VVVSSTGAYHYPNRKETPFALKIFEPVITRTIGKTTYDDQRRMEDIVRASGLDWTIVR
                     PSGLFDVPEPTNYVAGEVDPVGGFTARIDLADYLIALAGEPSAAGRTVVVSTTTDTPT
                     MWQMIRREAFKTAS"
     gene            6484..6834
                     /locus_tag="VFC01_35995"
     CDS             6484..6834
                     /locus_tag="VFC01_35995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZN85061.1"
                     /translation="MTTAYYTVTLIAIAANGFSGIAALLHFQPIMPGMAKAGVPVSWL
                     TFPIGTLKTLGAVGLAAGLLIRPLGVSAAVGLVLFFVCAIYTHIRAGDYGPQFGLAIG
                     FLGLNVAALALSLP"
     gene            complement(6947..7978)
                     /locus_tag="VFC01_36000"
     CDS             complement(6947..7978)
                     /locus_tag="VFC01_36000"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HZN85062.1"
                     /translation="MRIGTLTGGGDCPGLNAVIRAIVRTCDVRYGSTVVGFQDGWRGL
                     LENRRVNLANDDRNDRLLAKGGTMLGTARVHPEKLRAGLDQIKQTLDDNGIDVLIPIG
                     GEGTLTAAHWLSEENVPVVGVPKTIDNDIDCTDVTFGHDTALTVASEAIDRLHSTAES
                     HQRVMLVEVMGRHAGWIALNAGLASGAHMTLIPEQPYDVEEVCRLIKQRFVRGDSHFI
                     CVVAEGAKPAEGTMQLRQGGIDEFGHEKFTGVAQQLGVEVEKRINKEVRVTVLGHVQR
                     GGTPTPFDRVLATRFGVNAADAAHAGEYGMMVSLRGQDIGRVPLADAVRQLKLVPQSR
                     YDDAAEFFG"
     gene            complement(7996..9480)
                     /gene="gatA"
                     /locus_tag="VFC01_36005"
     CDS             complement(7996..9480)
                     /gene="gatA"
                     /locus_tag="VFC01_36005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZN85063.1"
                     /translation="MSTELTRLDAATLGAKIAAKEVSSTEATRACLDQIAETDEKYHA
                     FLHVAEEQALAEAARVDAAVAAGESLPSPLAGVPLALKDVFTTADMPTTCGSKILEGW
                     TSPYDATVTARLRAAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWNVDRVPGGSGGG
                     SAAALAAFQAPLAIGSDTGGSIRQPAALTATVGVKPTYGTVSRYGLIACASSLDQGGP
                     CARTVLDTALLHEVIAGHDPKDSTSIEAEVPDVVAAAKAGASGDLTGVRIGVVKQLRG
                     DGYQAGVLESFNAAVEVLTKLGAEVSEVDCPHFDYSLPAYYLILPSEVSSNLARFDAM
                     RYGLRVGDDGTHSAEEVMALTRAAGFGPEVKRRIMIGTYALSAGYYDAYYNQAQKVRT
                     LIARDLDEAYKKVDVLISPATPTTAFPLGEKVDDPLAMYLFDLCTLPLNLAGHCGMSV
                     PSGLSPDDNLPVGLQIMAPALADDRLYRVGAAYEVARGPLPTAL"
     gene            complement(9545..9844)
                     /gene="gatC"
                     /locus_tag="VFC01_36010"
     CDS             complement(9545..9844)
                     /gene="gatC"
                     /locus_tag="VFC01_36010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZN85064.1"
                     /translation="MPQISRDEVAHLARLARLALTDDELDSYAGQLDAILSHVSQIQA
                     VDVSGVDPTGNPLKDVNVFRTDDVEPCLTQEEALAEAPKAVDGRFAVPRILGEAE"
     gene            9907..10566
                     /locus_tag="VFC01_36015"
     CDS             9907..10566
                     /locus_tag="VFC01_36015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HZN85065.1"
                     /translation="MPSYLLRVQLEDRPGSLGSLAVALGSVGADILSLDVVERAAGYA
                     VDDLVVELPPGAMPDMLITAAEKLNGVYVDAIRPHTGLLEAHRELELIDHVAAADGTP
                     KRLQVLADEAPRVLRVGWCTVIGLTESRPKRFVGSPGAPETQAAETPWLPLDHAVALD
                     GDADWVPQVWRDMDTTLAAAPLGDHRIAIVLGRPGGPDFRPSEVARLGYLAGIIATIL
                     R"
     gene            complement(10583..11389)
                     /locus_tag="VFC01_36020"
     CDS             complement(10583..11389)
                     /locus_tag="VFC01_36020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN85066.1"
                     /translation="MIEVGYERRDRIAYITLNRPEAKNAVTPEMHDELCRLWAYFRDD
                     DQADVAILTGVGDAFCAGADLATYVPRNYVDAPPSRVREIVEMGFGGLTRGLHRITKP
                     TVAAVNGWALAGGLELALACDLRVASDRATFGSFEARRGFHHGDGGITRLVNICGVGV
                     AMQLVLTAEPIDAHRALQCNMVTKVVPHEALIEEAELMAHQILRNSQRAVRSAKETIL
                     EIIGKPLDDQLRIEGWNSYTCVDQDEARELLGRFFDRSDVGRTGSNSPES"
     gene            complement(11490..11744)
                     /locus_tag="VFC01_36025"
     CDS             complement(11490..11744)
                     /locus_tag="VFC01_36025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85067.1"
                     /translation="MAICDTCGNDYDKTFSVTWPDGHTATFDCIECAAAQLAPECAHC
                     GCRILGHGIETEDAIFCCAHCARKDSNADVNDRYPVPAGG"
     gene            complement(11781..>13562)
                     /gene="ligA"
                     /locus_tag="VFC01_36030"
     CDS             complement(11781..>13562)
                     /gene="ligA"
                     /locus_tag="VFC01_36030"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZN85068.1"
                     /translation="PEELTAWAARIKGEVGDDAHYLCELKIDGVALALVYRDGRLERA
                     ATRGDGRTGEDVTLNARTIEDVPEKLTGSKEFPVPDVLEVRGEVFFRVADFEDLNAGL
                     VAEGKPPFANPRNSAAGSLRQKNPAITARRRLRMICHGLGHAEGFTPKTLHDAYRALK
                     AWGLPVSDHTARVQGIDAVTEHIAYWGEHRHDVEHEIDGVVVKVDEIALQRRLGSTSR
                     APRWAIAYKYPPEEATTKLLDIKVNVGRTGRVTPFAFMEPVKVAGSTVSQATLHNASE
                     VKRKGVLIGDTVVIRKAGDVIPEVLGPVVDLRDGTEREFEMPTTCPECGTTLAPAKEG
                     DADIRCPNTRSCPAQLRERVFHVAGRGAFDIEGLGYEAATALLKSGVITDEGDLFTLG
                     RGDLLRTDLFKTKDGNLSANGARLLVNLHEAKSRPLWRVLVALSIRHVGPTAARALAT
                     EYGSLDAIMAASEDELSVVEGVGPTIAAAVVEWFTVDWHRAIVDKWHAAGVRMADERD
                     SSVARTLEGMSIVVTGSLTAFSRDDAKEAIVARGGKAASSVSKKTAYVVAGDSPGSKY
                     DKAVELGVPILDEDGFRRLLEDGPDGV"
BASE COUNT         2177 a         4592 c         4393 g         2400 t
ORIGIN      
        1 gtgtggacgt ggcccgaccg cccgggcgtc gcgggctgcg ccgccctcga cggcacggtg
       61 ctggtgaatc tcgtcaacac caagcagacg gtcgcggtgc ggctggcgcc ggacaccggc
      121 gcggtcaccg gtgacccgga ggtggtccgg caagaccagc acgggcacgc gtgggcgctg
      181 cagctctcgc ctgacggcaa cgtgtggggc gccacggtca acaagacgtc cggtgacgcc
      241 gagaagctcg atgatgtcgt gttcccgctg ttcccgcagg gtggcggatt cccgcgcagc
      301 aacgccgaca acacttaagg cgcaacgggt ttagccctcg acgaggcgca acccgacgat
      361 gccgaccacg atgatcgata ggaacagcag ccgcgccgcg cttgcggcct caccgaaaac
      421 gacgacgccc gcgatcacga ctccgaccgc gccgatgccg acccacacgg catagccggt
      481 gcctgccggc aggtgtcgaa gcgcgatggc aagcagcacg aagctcaaca ccgcgccgcc
      541 gacgccgatg acgctgggcc acagccgaga aaagccgtcg gactgcttca gcgctgtggc
      601 ccaaacgatc tcaagcaagc ccgcgcagat gagaacgagc cagggcatcg ccgttaggcg
      661 ttcgtgccgc cgtcgacggt cagcaccgaa ccggtgatgt tccgtgcgcc gtcgcctgca
      721 aggaaagcca ccgtgttggc gatgtcggtc ggattcgcat agtgccccaa cgcggttagc
      781 gcacgctggc cgtcggcggt cggcccgtcg gcgggattca tgtcggtatc cgtggaaccg
      841 ggctgcgcga ggttgacggt gatcccccgc tcaccgagat cgcgggccag gcccctggtg
      901 aagccgatga gtgcggactt gctcatcgag tagagcgtca cacccgaaaa cggcacgcgc
      961 tcagcgagat tgctgccgat gctgatgatc cgtccgccgg cggtcatgtg ccgcaccgct
     1021 gcctggctgg ccagaaaaac cgcccgggcg tggatgccca gcgcgcggtc gacctcctcg
     1081 agcgtcgtgt cgtcgatcgg cccgaacgga aagatgccgg cgttgttgac caagacgtcg
     1141 aggcggccga actcatcggc ggcctgtccg acagccgcct gcacctcgtt cgcatccgcg
     1201 ctgtccgcct tgatggccag cccgcgtcgg ccgtgtgcct tgatggtgtc gacgaccccg
     1261 tcggcgcgct ccttcgactg cgcatatgtg atcgccacgt cagagccgag ttcggccagc
     1321 cgtgccgcga tcgccgctcc gattccccgg ctcccgccgg tcactaaggc cacttttcca
     1381 gctagttcag tcatgttcgc ctcctattct gtgtcgttcg atacaaaact atattgatcc
     1441 aggtagactt gtcaatgaat tcggtgccgt tcgatacaga aatggggtgt ggcatggcgg
     1501 ctcgcggcag gccacgcgcg ttcgaccgga acggcgccct gcgcaaggcg atgaaagtgt
     1561 tctgggaaca cggctacgag gccgcctcca tcagcgatct gacggcagcg atgggcatca
     1621 attccccgag cctgtacgcc gcgttcggct gcaaggaggc gctgtttcgc gaggcggttg
     1681 atctctacaa cgaaaccgaa ggtgccgccg tcgctgcggc attgcgcgac ttacccactg
     1741 cccgcgaggc aatcacggaa gtgatgcgac accaggtgat ggcgtacacc gaccccgaaa
     1801 agccaagggg ctgcatgatc gttttgaccg cgaccaccta caccgagcgc agccacgcgg
     1861 tccatgaaca cctggccgac gagcggttgg ccaccgaaca cgactttcgg gaacggatcg
     1921 agcgtgggat cgccgacggc gacgttccag ccggcgctaa cgcggcgacg atcgccgcgt
     1981 tctacaacgc ggtcaaccag ggcatggcaa ttcaggcgcg cgacggcgcg gatcgatcga
     2041 agctgtcgac cgtcgcggag tcggcgattt cggcgtggga cgggctcgtc ggcgccggct
     2101 aactaggcgt gtatggcaag ccgttcgcgt gcttcggccg gcgtggtggt tgtccggcgc
     2161 tccggcggtc ccgcggacag gtgctgagcc aggacctcgg cggtctccgg cgcgatttgg
     2221 cgggccacgg cgagcatcgg ccgaaagttg ttggccgaca gcgccttgac ccatgcgcgg
     2281 gcaaccgggg tcacggcggc atcgacggcg gcgggctgat tgaaattgcc catgtgccgc
     2341 atccatttcg gcagggtggc gatcgccgcg ggggccaaca gccgactgcc tgcccacaac
     2401 cgaacaccgc ggtccctcgg cgtacgcagc aggtaatgca ttgcgcggtc ggcatgttcg
     2461 gaggtgcaca accggccgcg gacatcggtg aagtactgcc gcacgtcgtc gcgggacgcg
     2521 ggcacgtcgg ccggcttgca ggtctgcaac tcggcggcga tgcgcgactc cgcccagaac
     2581 cgtgactcct ccgcccgcga cagcggtccg ggtccgtact gctcgtagca cttgagcacc
     2641 gaatgccagc cggtgatgtg gatccacagc tgcgcgtccg ggttgttggc gctgtagcgc
     2701 gacccggtga tcggctcgac tcctttggcc ctcgcgtgga tccgcatcaa cgcatcggac
     2761 acctcgatcg cggtgcgacc gtcggacacc gcgacagtga ggaagtacgc cagcgtgcgg
     2821 tccaaccgtc ccgacggatc ggtgtagatg ccggcactgt cggccactgc cgcggcgagg
     2881 aacgggtcga agtgctcgag caccaccgcg cgctggaagc cgatcagcgc ggtaggcgac
     2941 gcccacacct tccagctcgg cgaatccggt ccgaagaacc cgtagtcgtc gcgccgaatc
     3001 gactcgatca tggtcactac ctctgctttc ccctgaatac aacgccaccg tagcaattac
     3061 cccgcatgaa tacaacggtg gcgtaatatt gtctgcgtga tcggaccacg gccgcggcgc
     3121 aagtacgcaa aacgcctgcc tcgtgaacag cggcgtgagc agttgttgga cgcggccatg
     3181 accgtgctcg gcggctgtca gcttcacgag ctgagcatgg aggcggtggc cgaagcggcc
     3241 ggcgtcggaa agccggtgct gtacaccgta tttcggacgc gagccgaact ggtctccgcg
     3301 ttattgcggc gcgaacatca gcggggactg gagcaggtgc tcgccggaat gcccgacgac
     3361 ctgaccgcgc cgggcccgac gggcgcatac gcggcgacgg tttcggcgtt cctccgcgtg
     3421 gtgctggaga atcccatgcg ctggcggctc atcctgacgg tgcccgacag cgcgccgcgc
     3481 gaataccgcg atgacctgcg gcgtgctcgg tcatcgatcc tggctcaggc cgagaacctg
     3541 gccaaggccg gtattgcgct ggagccccgg ctcgccgacc tcgatccggc cttgctcggc
     3601 cacaccatgc tgtcgttcgc cgaaatgctg ggacgactgg cgatcaacga tcccgagacc
     3661 tatccccggg agcggctcga ggagttcgcg gccgcggcga tgggaatccg gcgctaactg
     3721 caggcggcga gcaccagctc gcgcacccgc gcggcgtcgg cctggccctt ggtcgccttc
     3781 atcacggcgc cgacgatcgc gcccgccgca gccaccttgc cgccgcggat cttctcgacg
     3841 acatcggggt tggcggccag cgcctcgtca accgcggcct gcagtgccga gtcgtcgcgc
     3901 acgacctcca ggcctcgtgc cgccatcacc tgttcgggct caccctcgcc ggccaacacg
     3961 ccctcgacga cctggcgagc caacttgttg gacagcttgc cctcgtcgac gagcttgacc
     4021 accgcagcca cctgcgccgg cgtgatcggg agtgcgtcga gctcgacacc cgcttcattg
     4081 gccttctgca ccaagaagtt tccccaccaa gcgcgagcgg attcgctcga tgcgccctgc
     4141 tggacggtgg ccgtgatcaa gtcgatcgcg ccgatgttga ccaggtcgcg catcacctcg
     4201 tcggagatgc cccactcctg ctgaatcctc ttgcgctgca accacggtag ctccggaatc
     4261 gtctgacgca gccgctcgac gacctcagcg tcaggggcca ccggttccag atccggttcc
     4321 gggaagtagc ggtagtcctg cgcggtttcc ttgctgcggc ccggtgaggt gtagccgtcc
     4381 tcgtggaagt gacgcgtttc ctgcacgacc cggccacctg agtcgaggac ggccgcctga
     4441 cggcgcatct cgtaacggac cgccacctca acacttttca gcgagttgac gttcttggtc
     4501 tcggtgcggg tgccgaactc tttctggccg gtcggcttga gagacacgtt ggaatcacag
     4561 cgcatcgacc cctggtccat ccgaacatcg gaaacgccca acccgcgcaa cagatcccgc
     4621 agcgccgtca cgtaggcgcg ggcgatctct ggcgcgcgtt caccggtgcc ctcgatcggc
     4681 ttggtgacga tctcgatgag cggcacgcca gagcggttgt agtcgatcag cgatgtcgtc
     4741 gcgccctcga tgcgaccggt ctcgctgcca aggtgcgtga gcttgccggt gtcctcttcc
     4801 atgtgggcgc gctcgatgtc gacccgccac gtggtgccgt cgtcgagcgg aacgtcgaga
     4861 tagccgttga tcgcgatcgg ctcgtcatac tgcgagatct ggtaattctt cggcatgtcc
     4921 ggatagaagt agttcttccg ggcgaaccgg caccacggca cgatctcgca gcccagtgcc
     4981 agaccgatgc ggatcgccga ctccaccgcc gtctggttga gcaccggcaa tgagccgggc
     5041 aggccgaggc agaccgggca tacctgggtg ttgggttctg cgccgaactt gttggcgcag
     5101 ccgcagaaca tcttggtctc ggtcgacagc tcgacgtgca cctcaaggcc gagcacgggt
     5161 tcgtagcgtg ccacgacgtc gtcgtaatcg accagttcgg cagcggcgac agtcatgcgt
     5221 ccgatcgtag tgggaccgcc ctagccgagt ttggggtgca cgcccgggat gagacccgcc
     5281 gcggtcgaca tccgctgcag gtgctgcaac accgccttgc gctcgtcgtc cgtcagcaag
     5341 gcggtgattt cgtcctcctg ggttcgtcct tcggcatcca gcttcgggag cagctcgcgg
     5401 gccttatcgg tcaatgtcac cgcgaacgca cgtcggtccg cagggtgtgg catccgtttg
     5461 accagtccct cacgttccaa cttgtcgaca accgtgacca tcacgttgcg gtgaatcccg
     5521 agctgcgtcg acagctcgcg ctgcgagcgc ccgtcgtcct cgctcagcgc cttcatcacg
     5581 gcatatgcgc gcggttcgac tccgaacggc tccaagcggt gcacgaagtc ctcggcgaga
     5641 tagatgccga gctgcgaaag caagaacgga atgcgctggt gaagtccggc gattccagct
     5701 tcgcggtcgg gcatgcgcca gacattagca cccttcatga tagtcatgta gagtgataat
     5761 catgtggaag gatcgatcag gaggagcgat ggacatcgtt gttttcgggg cgaacggaca
     5821 gaccggcaga ctgctgactc gacgagctct cgacaagggg cactcagtgg tggccgtgac
     5881 ccggcaccct gacgacttcc cgttatccca ttcgctgctg accgttgccc gcgccgacgt
     5941 gcgcgagccc tcgtcactag tcgatgtcgt gcgcggcgcc gatgccgtgg tgtccacact
     6001 tggcgtaccg ttcaccaccc gttccatcga cacgtactcg gtgggtgtgc gcaacatcgt
     6061 cgacgcgatg caaatgactg gcgtgcagcg cttggtcgtg gtgagttcga cgggtgcgta
     6121 ccactacccg aaccgcaagg aaacaccttt cgcgctgaag attttcgagc cggtgatcac
     6181 ccgcaccatc ggcaagacca cctacgacga tcagcgccgc atggaggaca tcgttcgggc
     6241 aagcggcctg gattggacga tcgttcggcc gtcgggacta ttcgacgtgc cagagccaac
     6301 gaattacgtt gcgggcgagg tagatccggt cggaggcttc accgcccgca tcgaccttgc
     6361 ggactacctg atcgcgctgg cgggtgagcc gtccgcggcc ggtaggactg tcgtcgtctc
     6421 cactaccacg gacactccga cgatgtggca gatgatccgg cgcgaagcgt tcaagaccgc
     6481 ctcatgacaa ccgcctacta caccgtcacg ctgatcgcca ttgccgccaa cggtttttcg
     6541 ggcatcgcgg cgctgcttca tttccagccg ataatgccgg gcatggccaa ggccggagtc
     6601 cccgtgtcgt ggctgacctt tccgatcggg acgttgaaga cccttggcgc agttgggctt
     6661 gccgcgggac tgctgattcg tccgcttggc gtttcggccg ctgtcggcct agtgctgttc
     6721 tttgtgtgcg ccatctacac gcatatccgg gcaggtgact acggcccgca gttcgggctc
     6781 gcgatcggct ttctcggtct caacgttgcg gcgcttgccc tttcgctgcc ctagcgcgag
     6841 cagacgcaaa gtcccccaaa tcctcggcct gtcggggatt ttgcgttccc ctactcccca
     6901 gcttgcgcgt gggcccagcg gtgtggtccc agctcgccgg aagaactcag ccgaagaatt
     6961 cggcggcgtc gtcgtaacgg ctctgtggca ccagcttcaa ctggcgcacc gcgtcggcca
     7021 agggcacacg cccgatgtcc tggccacgca gggacaccat cattccgtat tcgccggcat
     7081 gcgcggcgtc ggcggcattg accccgaagc gggtggctag cacgcggtcg aacggcgtcg
     7141 gcgtgccgcc gcgctgcaca tgaccgagca cagtgacccg cacctccttg ttgatccgct
     7201 tctccacctc gacgcccagt tgctgcgcga ccccggtgaa cttctcgtgc ccgaactcgt
     7261 cgataccgcc ctgcctcagc tgcatcgtgc cctcggcggg cttcgcacct tcggccacca
     7321 cgcagatgaa atgtgaatcg ccgcgcacga agcgctgttt gatcagccga cacacctctt
     7381 cgacgtcgta gggctgctcg gggatcagcg tcatgtgggc accggatgcc aggccggcat
     7441 tcaatgcgat ccatccggcg tggcggccca tcacctccac cagcatcacc cgctgatggg
     7501 attcggcggt gctgtgcaac cggtcgatgg cctcgctggc gacggtcagc gcggtgtcat
     7561 gcccgaatgt cacgtcggtg caatcgatgt cgttgtcgat cgtcttcggc acgcctacaa
     7621 caggaacgtt ctcctcggac agccagtgcg cggccgtcag ggtgccctcg ccaccgatcg
     7681 ggatgagcac gtcgatgccg ttgtcgtcca gcgtctgctt gatctggtcg agcccggcgc
     7741 gcagcttctc cgggtgcacc cgggcggtgc ccagcatggt tccgcccttg gccagcagcc
     7801 ggtcattgcg gtcatcgttg gccagattga cgcgccggtt ctccagcagt ccacgccagc
     7861 cgtcctggaa gccgacgacc gttgagccgt agcgcacgtc gcaggtccgc acgatcgcgc
     7921 ggatcaccgc gttcaggccg ggacagtcgc caccgccggt aagtgttccg atgcgcatgg
     7981 gttcatcctc ccccgttaca gcgcagttgg cagcggacct cgggcaactt cgtaggcggc
     8041 gcccacccgg tagagccggt cgtcggccag cgccggcgcc atgatctgca ggcccaccgg
     8101 caggttgtcg tccggtgaca ggcccgacgg caccgacatt ccgcagtggc cggccaggtt
     8161 cagcggcagc gtgcacaggt cgaacaggta catcgccagg gggtcgtcta ccttctcccc
     8221 cagcgggaac gcggtggtcg gtgtggcagg cgagatcagc acgtcgacct tcttgtaggc
     8281 ctcatcgaga tcacgcgcaa tcagcgtgcg taccttctgc gcctggttgt agtaggcgtc
     8341 gtaatacccc gccgagagtg cgtacgtgcc aatcatgatg cggcgcttga cttctgggcc
     8401 gaatccggcg gcccgggtca gcgccatgac ctcttcggcg ctgtgcgtgc cgtcgtcgcc
     8461 gacgcggagc ccgtagcgca tcgcgtcgaa gcgcgcgagg ttcgacgaca cctccgacgg
     8521 caggatcagg taatacgccg gcagtgagta gtcgaagtgc gggcagtcga cctcgctgac
     8581 ctcggcgccc agcttggtca atacctccac ggccgcgttg aacgactcca gcaccccggc
     8641 ctgatagccg tcgccgcgta actgcttgac cactccgatc cgcacacccg tcaggtcgcc
     8701 ggacgcgccg gccttggccg cggcaaccac gtcgggcacc tcggcctcga tggaggtgga
     8761 gtccttgggg tcgtgcccgg cgatgacctc gtgcagcagc gctgtgtcga gcaccgtgcg
     8821 ggcgcacggc ccgccctggt ccagcgacga cgcgcacgcg atcagcccgt accgcgacac
     8881 cgtgccgtag gtgggcttca cgccgacggt cgccgtcagc gcggcgggtt ggcggatcga
     8941 cccacccgtg tccgatccga tggccagcgg cgcctggaac gcagcgaggg ccgccgcgct
     9001 gccgccgccc gatccgccgg gcacccggtc gacgttccac gggttgcggg tcgggccgta
     9061 cgcggagttc tcggtggagc tgcccatcgc gaactcgtcc atgttggtct tgcccaggat
     9121 cgggatgccg gccgcgcgca gccgcgcggt gacggtggcg tcgtacggcg acgtccaccc
     9181 ctcgaggatc ttcgatccgc aggtggtcgg catgtcggcg gtggtgaaca cgtccttgag
     9241 cgccaaaggc accccggcca gcggcgacgg cagcgactcg cccgcggcga cggccgcgtc
     9301 cacccgcgcc gcttcggcga gcgcctgctc ctccgccacg tgcagaaacg cgtggtactt
     9361 ctcgtcggtc tcggcgatct gatcgaggca cgcccgcgtc gcctccgtgg acgacacctc
     9421 cttggcggcg atcttggcgc ccagcgtggc ggcgtcgagc cgggtcagct cggtgctcat
     9481 gcctcgcctc ccgctcgctc cggtcctcgc ttcactgcga tcctcactcg cgctcgcctt
     9541 cgggtcattc ggcctccccc agaatccgcg ggaccgcgaa ccggccgtcg accgccttag
     9601 gcgcctcggc cagcgcttct tcctgcgtca ggcacggctc gacgtcgtcg gtccggaaca
     9661 cgttgacatc cttgagcggg ttaccggtgg ggtcgacacc gctgacgtcg acggcctgga
     9721 tttggctgac gtggctcagg atggcgtcca gttgcccggc gtagctgtcc agctcatcgt
     9781 cggtcagcgc gaggcgcgcg aggcgcgcca ggtgcgcgac ttcgtctcgg gagatctgcg
     9841 gcacggcagc caagcgtagt caccggccgg aatgccggct caggcgcgct gtgcgacagt
     9901 gtgcgcgtgc cttcgtatct gctgcgggtc cagctggagg atcgaccggg cagcctgggc
     9961 tcactggccg tcgcgctcgg ttcggtgggc gccgacatcc tgtccctcga cgtcgtcgag
    10021 cgcgctgcag gctatgcggt cgacgatctc gtggtcgaac tgccgccggg cgccatgccc
    10081 gacatgctga tcaccgcggc cgaaaagctc aacggtgtct acgtcgacgc catccgcccg
    10141 cataccggac tgctcgaggc gcatcgggag ctcgaactga tcgaccacgt cgccgcggcc
    10201 gacgggacgc ccaagagact gcaggtgctc gccgacgagg cgccgcgggt gctgcgggtc
    10261 gggtggtgca cggtgatcgg actcaccgaa tcgcgcccca agcgcttcgt cggtagtccc
    10321 ggcgctcccg agactcaggc ggccgaaacg ccgtggctgc cgctcgacca cgccgtggcc
    10381 ctcgacggcg acgccgactg ggtgccgcag gtgtggcgag atatggacac gactctcgcg
    10441 gccgctccgc ttggtgacca ccgcatcgcg atagtgctgg gccgcccggg cgggccggac
    10501 ttccggccgt cggaggttgc ccggctgggc tacctggcag ggatcatcgc gacgatcctg
    10561 cgctgaccct cctcagccgc ccttaactct ctggcgaatt cgaccctgtt cggccgacat
    10621 ccgatctgtc gaagaaccga cccaacaatt cgcgagcctc gtcctggtcg acacacgtgt
    10681 aggaattcca gccctctatc cgaagctgat cgtccagcgg cttgccgatg atctcgagga
    10741 tcgtttcctt tgcagatcgc acagcccgct gcgagttgcg caggatttgg tgagccatca
    10801 gctccgcttc ctcgatcagc gcctcatgcg gaacgacttt tgtgaccatg ttgcattgca
    10861 gcgcccgatg cgcgtcgatc ggttcggcag taagaaccag ttgcatcgct acaccgacac
    10921 cgcagatgtt gaccagccgc gtgattccgc cgtcaccgtg atggaacccg cgccgcgcct
    10981 cgaaggatcc gaacgtggcg cggtcggaag ccacgcgcag atcacatgcc agcgcgagct
    11041 caaggccgcc ggccagggcc cacccattta cggctgcgac ggtcggtttg gtgatgcgat
    11101 gcaggccgcg agtgaggccg ccgaacccca tctcgacgat ttcgcgaacc ctgctcggag
    11161 gggcgtcgac gtagtttctc ggaacgtagg tcgcgaggtc tgcgccggcg cagaaggcgt
    11221 cgcctacgcc ggtgagaatc gcaacgtccg cctggtcgtc atcgcggaag taggcccaaa
    11281 gccgacacag ttcgtcgtgc atctccggtg tgaccgcatt tttcgcctcc ggccggttca
    11341 gcgtgatgta ggcgattctg tcccgacgct cgtaaccgac ctcgatcatc ggtcaatcct
    11401 gcgacgacga tctcagccct gcgtccagat cgacgttttg acggcctcaa ctcgtcgcac
    11461 tcgtcctgcg aagagtcggt ttaggcgaat cagcctccgg cgggcaccgg gtagcggtcg
    11521 ttgacgtcgg cattgctgtc cttccgagcg caatgcgcgc aacagaagat cgcgtcctcg
    11581 gtctcaatgc catggcccag aatccggcat ccgcagtgcg cgcattcggg tgcaagttga
    11641 gcggccgcgc actcgatgca gtcgaatgtc gcggtgtgac cgtcgggcca ggtcacgctg
    11701 aatgtcttgt cgtagtcgtt gccgcaggta tcgcagatcg ccatgccaac gcgggttccc
    11761 ggtcgaggac ggcgacaaac tcagacgccg tcgggcccgt cctccaacag ccggcggaag
    11821 ccgtcctcgt cgaggatcgg cacaccgagt tcgacggcct tgtcgtactt cgatcccggt
    11881 gagtcgcctg ccaccacata ggcggtcttc ttcgacactg agctggccgc cttcccgccg
    11941 cgcgcgacga tggcctcctt ggcgtcgtcg cgtgagaacg cggtgagcga tccggtgacg
    12001 acgatcgaca taccctcgag cgtgcgggcg acgctgctgt cgcgttcgtc ggccatccgc
    12061 accccggccg catgccactt gtcgacgatc gcgcggtgcc agtcgacggt gaaccattcc
    12121 acaacggcgg ccgcgatggt cggaccaacg ccctcgacca cggaaagctc gtcctccgac
    12181 gccgccatga tcgcgtcgag gctgccgtat tcggtggcca gggcgcgagc agcagtcggg
    12241 ccgacgtggc ggatcgacag tgcgacgagc acccgccaca gcgggcggga cttcgcctcg
    12301 tgcagattca cgagtagccg tgcaccgttg gccgacaggt tgccgtcttt ggttttgaac
    12361 agatcggtgc gcagcaggtc gcctctgccg agcgtgaaca ggtctccctc gtcggtgatt
    12421 actcccgact tgagaagcgc ggtcgccgct tcgtagccga ggccttcgat atcgaacgca
    12481 ccgcggcccg cgacgtgaaa cacgcgctcc cgcagctgtg ctgggcacga ccgtgtgttc
    12541 gggcagcgga tgtcggcgtc gccctccttg gcgggtgcca gcgtcgtgcc gcactcggga
    12601 catgttgtgg gcatttcgaa ttcgcgttcg gtgccatcgc gcaggtcgac gacgggaccg
    12661 agcacctcgg gaatcacgtc accggccttg cggatcacca ccgtgtcgcc gatcagcacg
    12721 cctttgcgtt tgacctccga tgcgttgtgc agcgtggcct ggctgaccgt cgagccggcg
    12781 accttgaccg gctccatgaa cgcgaacggc gtgacccggc cggtgcgccc gacgttgacc
    12841 ttgatgtcga gcagcttggt ggtcgcttcc tcgggcgggt acttgtaggc gatcgcccag
    12901 cgcggcgccc gcgatgtcga tccgagccgt cgctgcagcg cgatttcgtc gactttgacc
    12961 accacaccgt cgatttcgtg ttcgacgtcg tgccggtgtt caccccagta cgcgatgtgt
    13021 tcggtgaccg catcaatccc ctgcactcgg gcggtgtggt cggacaccgg cagaccccac
    13081 gccttgagcg cgcggtaggc gtcgtgcagc gtcttcggcg tgaagccctc ggcgtggccg
    13141 aggccgtggc agatcatccg gagtctgcgc cgggcagtga tcgcggggtt cttctgccgc
    13201 agtgagcccg ccgcgctgtt ccgcggattg gcaaacggcg gcttgccctc ggcgaccagg
    13261 ccggcgttga ggtcctcgaa gtcggcgacc cggaagaaca cctcgccgcg aacctcgagc
    13321 acatccggca ccggaaactc cttggagccg gtcagcttct ccggcacatc ctcgatggtg
    13381 cgcgcgttga gcgtgacgtc ctcaccggtg cggccgtcac cacgggtcgc ggcgcgctcc
    13441 agcctgccgt cgcggtacac cagcgccagc gcgaccccat cgatcttcag ctcgcacagg
    13501 taatgcgcgt cgtcaccgac ctcgcccttg atccgggcag cccaagcggt caattcctca
    13561 gg
//
LOCUS       DASRJA010000798         5431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_986315,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000798 DASRJA010000000
VERSION     DASRJA010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986315"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFC01_36035"
     CDS             complement(<1..610)
                     /locus_tag="VFC01_36035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN85069.1"
                     /translation="MPMTFETILLDVEATSHIATITLNRPDSLNAFNRTMCDEMAAAW
                     RVVKLDETVNAVVLRAAGDRAFSAGLDIKSSYGQPDNVWNHEDPGEALSPKWQKMWKP
                     VVCAVQGMCTAGAFYFVNESDVVICSEDATFFDSHVSAGLVCALEPIGLMRRVGLGET
                     LRIALMGNDERVSADTALRIGLVSEVVSLDRLWARAHEIAAAI"
     gene            complement(601..1398)
                     /locus_tag="VFC01_36040"
     CDS             complement(601..1398)
                     /locus_tag="VFC01_36040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN85070.1"
                     /translation="MKYIKYEVDGHKVTITLNRPDALNALSPAMISELRAAYDEAEND
                     DNIWTLIVTGTGRAFCTGADVGEIPEDGRVIYERPYLSTYDQWEAPQEGTPPFRRMAK
                     PVLTAINGLCCGAGLDWITTGDIAIASDKATFFDPHVSIGLVAGREMVRLARVLPRNI
                     ALRMALMGKHERMSAQRAYELGMISEVVEHDKLLERAHEIADVVNSNAPLAVRGTRLA
                     IHKTLDLPLHEAEILAETFRERVVRTEDALEGPKAFMEKRAPNWQCR"
     gene            complement(1403..2209)
                     /locus_tag="VFC01_36045"
     CDS             complement(1403..2209)
                     /locus_tag="VFC01_36045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN85071.1"
                     /translation="MTDVEADGSVTASRNGDVLELALDRPSRRNALSHTMIDSLVGLL
                     TGAATDDSLRAIHIRGTGSDFCAGADWVATNNAGQRPRTGDLVRRIPHTANRVIELVH
                     GIQLPVVCSVQGWAVGLGCNLALAADFTVATDEAVFWEPFVGRGFSPDSGSTWLLPRL
                     VGLARAKEMLLLGEKVSGRDAADWGLIHRCVSAAEIDDVAGQLVTRLASGPTVAIGLA
                     KQAINYGQHATLSQAMTQELFNVELSCRTADFKEGLAAFRDKRTPEFGGR"
     gene            2312..2962
                     /locus_tag="VFC01_36050"
     CDS             2312..2962
                     /locus_tag="VFC01_36050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85072.1"
                     /translation="MAEGTPRYGEQPLPQTVAAAGALRRLTGLLLSLEHEHPTVDAML
                     AQFADWERELATAVPPDLTPRIGAGGEDQRVYLDHAFDVGAYNPAFPEYTFDHLDDET
                     ASGRVTFPLVYEGPPGLVNGGFLAVFFDCITQHQSCAARRTGKTRSLTVTFRRPTPVL
                     TELRFDIERSEHDGRVTSTARLMLGDEVVCIGEFKTVATDPDKLTGFQFGKRRVKN"
     gene            2962..3744
                     /locus_tag="VFC01_36055"
     CDS             2962..3744
                     /locus_tag="VFC01_36055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZN85073.1"
                     /translation="MVVQRIRQPRVAEIVASRLREDILSGRLKEGDVLPSQESLFSEF
                     GVSPPALREAIHILETDGLISVRRGNMGGAVVHQPSAERTAHMISMVLQSRSSTPADV
                     SGALLHLEPICAGMCAAREDRLTEVVPYLEAEIQLQTEQFDDLSRYVRNARRFHEAIV
                     SRCGNEPMILLIGSLELIWSAHESSVWSDEGDPMSRKTMRAALRDHQRLLDAIRDGDS
                     TRTVRLAQDHLAAARRNTLAVGNDKTIEAKLISSTNHQEPGR"
     gene            3741..4604
                     /locus_tag="VFC01_36060"
     CDS             3741..4604
                     /locus_tag="VFC01_36060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZN85074.1"
                     /translation="MTDDRVLYNVDSEKRIATITFNNPSQRNSYDATMRDTVARHLDR
                     VAEDDDITVVLLRGADGVFSTGADMNNAYGWYGDRDAKPAKSRPSQRRRLTVDRKSFS
                     FYHNLMGFPKVTVGEISGYALGGGFEMALMTDISVIGRDTKIGMPATRFLGPALGSLH
                     MFFHRLGPVLARRLLLTGDIIEAGAIEHLGIFTDTCEPASVPARARYWAEKAAKMPAD
                     GVVIAKEAFRLVEQSQAYQGEEVASYLFHAFGTNLQFAPGEFNFVKTRAQHGTKEAFR
                     LRDEHFFVPEP"
     gene            4688..>5431
                     /locus_tag="VFC01_36065"
     CDS             4688..>5431
                     /locus_tag="VFC01_36065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZN85075.1"
                     /translation="MPTPVIVGAARTAIGRSFKGTLVNTPPETLITTVLPEVVRRSGV
                     DPSAFDDIIFAESHYGGGDLARYAADATGMQGVPGQSVNRHCAGSLTAIGNAAAQIGS
                     GMERALIAGGVQSLSMTPLTNWRIPGPELKFEERWMPPTHVETPDAPCKDMSITVGWN
                     TAQAVGITREEMDAWAARSHQRAIAAIDAGKFVDEIIPLKVQQFDGSVVDFSVDEHPR
                     RDTTVEKLAALKPLHPEIEGFSITAGNSSG"
BASE COUNT          962 a         1773 c         1772 g          924 t
ORIGIN      
        1 tgatcgccgc cgcgatctcg tgcgcgcgcg cccacagccg atcgagggag acgacctcgg
       61 acaccaatcc gatgcgcagc gccgtgtcgg cgctgacgcg ttcgtcgttg cccatcaacg
      121 cgattcgcag ggtttcgccg aggccgaccc ggcgcatcaa cccgatcggt tccaatgcgc
      181 acaccaatcc ggcgctgacg tgcgagtcga agaacgtcgc atcctcggag caaatcacca
      241 catcggactc gttgacgaaa tagaacgcgc cggcggtgca catgccttga acagcacaca
      301 cgacgggttt ccacatcttc tgccatttcg ggctcagcgc ctcacccgga tcttcgtgat
      361 tccacacgtt gtccggttgg ccgtaggacg acttgatgtc caggccggcg ctgaacgcgc
      421 gatcgcccgc cgcacgaagc acgaccgcgt tcaccgtctc gtcgagtttg acaactcgcc
      481 acgccgcggc catctcgtcg cacatggttc ggttgaacgc gttgagcgaa tcgggccggt
      541 tcagtgtgat ggtcgcgatg tgactcgtcg cctccacgtc gagcaggatc gtctcgaagg
      601 tcatcggcat tgccagttcg gggctcgttt ttccatgaac gccttgggcc cttcgagtgc
      661 gtcttcggtg cgcacgacgc gctcgcggaa tgtttcggcg aggatctcgg cttcgtgcag
      721 cggaaggtcc agcgtcttgt ggatcgccag ccgagtgccg cgaacggcca gcggcgcatt
      781 cgaattcacg acgtcggcga tctcgtgggc gcgctcgagc agcttgtcgt gttcgacgac
      841 ctcgctgatc atgccgagct cgtacgcgcg ctgcgcgctc atccgttcgt gcttgcccat
      901 cagcgccatg cgcagcgcaa tgttgcgggg caggacgcgg gccagccgca ccatctcgcg
      961 gccggccacc aggccgatgc tcacatgcgg gtcgaagaac gtcgccttat cggaggcgat
     1021 cgcgatgtcg ccggtggtga tccagtccag ccctgcgccg cagcacaatc cgttgatggc
     1081 ggtcagcacg ggcttggcca tcctgcggaa cggcggagtg ccctcttgcg gggcctccca
     1141 ctggtcgtag gtggacaggt agggccgctc gtaaatcacc cggccgtcct cggggatctc
     1201 gccgacgtcg gcgccggtac agaacgcgcg gccggtgccg gtgacgatga gggtccagat
     1261 gttgtcgtcg ttttcggcct cgtcgtacgc ggcgcgaagc tcactgatca ttgccggact
     1321 cagcgcgttg agcgcgtcgg gccggttcag cgttatcgtc accttgtgcc cgtcgacctc
     1381 gtacttgatg tacttcatct agtcaccttc caccgaactc cggtgtacgt ttgtcgcgga
     1441 acgctgccag gccctctttg aaatcggctg tccggcagga caactccacg ttgaacagct
     1501 cctgcgtcat cgcctgactc agggtggcgt gctgaccgta attgatcgct tgcttggcca
     1561 gcccgatggc caccgtcggc ccggacgcca gccgggtcac cagttgcccg gcgacatcgt
     1621 cgatttcggc agcgctcacg cagcggtgaa tcagacccca atccgccgcg tcgcgtccgc
     1681 tgaccttctc gccgagcagc aacatctctt tggcgcgcgc caggccgacg agccggggca
     1741 gcagccaggt cgagccggag tcgggggaga agccgcggcc gacgaacggc tcccagaaca
     1801 ccgcttcgtc ggtggcgacg gtgaagtccg ccgccagggc caggttgcag ccgaggccga
     1861 cggcccaccc ctgcacgctg cagaccacgg gcaactggat gccgtggacg agctcgatca
     1921 cgcgattggc ggtgtgtgga atgcgtcgaa ccaggtcacc ggtgcggggt cgctggccgg
     1981 cgttgttggt ggccacccag tccgctccgg cgcagaaatc gctgccggtg ccgcgaatgt
     2041 ggatggcccg cagcgaatcg tcggtcgcgg cgccggtcag cagtccgacc aacgagtcga
     2101 tcatcgtgtg gctgagcgcg ttgcgtcgcg acgggcggtc gagggcgagc tcaaggacat
     2161 cgccgttccg cgatgccgtc acggagccgt cggcttcgac gtcagtcacc gttgaatccg
     2221 ccaatcctta gctatacggt tagccataca gtattgctat cctgtacaag ttagcaagga
     2281 aagcgcgcta acggaacacg gaagagggcc catggccgaa ggcacaccgc gatacggcga
     2341 gcagccgcta ccgcagacag tcgccgccgc cggggcgctc cggcgactga ccgggctcct
     2401 gctgtcgttg gagcacgagc atccgaccgt ggacgccatg ctcgcgcaat tcgccgactg
     2461 ggagcgggag ttggcgaccg cagtgccgcc ggacctcaca ccgcgaatcg gagcaggcgg
     2521 tgaggaccaa cgcgtgtacc tggatcacgc gttcgacgtc ggcgcgtaca accccgcttt
     2581 ccccgagtac accttcgacc atctcgacga cgagacggcc tccggccgcg tcaccttccc
     2641 gctggtttac gagggcccgc cgggattggt gaacgggggc tttctcgctg ttttcttcga
     2701 ctgcatcacg caacaccaga gctgcgcggc aaggcgcaca ggtaagacgc gctcactcac
     2761 cgtgaccttc cgtcggccca cgccggtcct caccgaactc cgcttcgaca tcgagcggtc
     2821 ggaacacgac ggcagggtca cgtcgacggc gaggctgatg ttgggcgatg aggtggtatg
     2881 catcggtgag ttcaagacgg tggcgacgga ccccgacaaa ctaaccgggt tccaattcgg
     2941 caagcggcgg gtcaagaact agtggtcgtg caacgcattc gccaacctcg ggtggccgag
     3001 atcgtggcct cgcggctgcg ggaggacatc ctgtcggggc ggctcaagga aggcgatgtg
     3061 ctgccgtcgc aggagagcct gttctccgag ttcggagtga gcccgcccgc gctgcgcgag
     3121 gcgatccaca ttctggaaac cgacgggctg atctcggtgc gtcgcggcaa catgggcggg
     3181 gcggtcgtcc accagccctc cgccgagcgg accgcccaca tgatcagcat ggtgctgcag
     3241 tcgcggtcgt cgacgccggc cgacgtgagc ggcgcgctgc tgcacctgga accgatctgc
     3301 gccgggatgt gcgcggcgcg tgaggaccgg ctgaccgaag ttgtgcccta cctcgaagcc
     3361 gaaatccagc tccagacgga gcaattcgac gacctatcgc ggtatgtccg caacgcgcgg
     3421 cggtttcacg aagcgatcgt ctcgcggtgc ggtaacgagc cgatgatcct gctgatcggc
     3481 tcgctggagc tgatctggtc ggcgcacgag tcctcggtgt ggagcgacga aggcgatccg
     3541 atgtcgcgca agaccatgcg cgctgccctg cgggatcacc agcggctgct ggacgccatc
     3601 cgtgacggcg actcgaccag gacggtccgg ctcgcgcaag accacctggc cgccgcccgg
     3661 cgcaacaccc tcgccgtcgg caatgacaaa accattgaag ccaaactgat ctcgagtacg
     3721 aaccatcagg agcccggcag atgacagacg accgcgtgct ttacaacgtc gattccgaaa
     3781 agcgaatcgc gaccatcacg ttcaacaacc caagccagcg caattcctac gacgcgacga
     3841 tgcgcgacac cgttgcgcgg cacttggatc gcgtcgccga ggacgacgac atcaccgtgg
     3901 tgctgctgcg cggcgcggac ggtgtgttca gcaccggcgc ggacatgaat aacgcgtacg
     3961 gctggtacgg cgaccgcgac gcgaaaccgg ccaagagccg gcccagtcag cggcggcgtt
     4021 tgaccgtgga ccgcaagtcg ttcagcttct accacaatct gatgggcttc ccgaaggtca
     4081 cggtggggga gatcagtggt tatgcgctcg gcggcggttt cgagatggcg ttgatgaccg
     4141 acatctccgt gatcggacgc gacaccaaaa tcggcatgcc cgccactcgg ttcctcggac
     4201 ccgcactggg cagtctgcac atgttctttc accggctcgg gccggtgctg gcgcggcggc
     4261 tgctgctgac tggcgacatc atcgaagcgg gtgccatcga gcacctcgga atcttcaccg
     4321 acacatgcga gcccgcgtcg gtgcccgcga gggccaggta ttgggcggag aaggccgcga
     4381 agatgcccgc cgacggtgtc gtcatcgcca aggaagcgtt ccggctcgtc gagcagagcc
     4441 aggcctatca gggtgaggaa gtcgccagct acctgttcca cgcgttcggg acgaacctgc
     4501 agttcgcgcc gggcgagttc aacttcgtca agacgcgggc gcagcacgga accaaggagg
     4561 cgttccggct gcgtgacgag cacttcttcg tgccggagcc ttaagtgacg ccggtcacct
     4621 actagcagcg atacattatc tgctactttt gtaacgtcgt taacgccgaa agtcgaggtc
     4681 gagaaacatg ccaacacccg tcatcgtcgg tgctgccagg acggccatcg gccggtcgtt
     4741 caaggggacg ctggtcaaca caccacccga gacgctgatc acgaccgtgc tgcccgaggt
     4801 ggtccgccgc tccggcgtcg atcccagcgc gttcgacgac atcatcttcg ccgaatcgca
     4861 ttacggcgga ggcgatctcg cgcgctacgc ggccgacgcg accggcatgc aaggtgttcc
     4921 cggtcagtcg gtgaaccggc attgcgcggg cagcctgacc gcgatcggca acgcggccgc
     4981 gcagatcggc tccggcatgg agcgcgcgct catcgccggc ggcgtgcagt cgctgtcgat
     5041 gacgccgttg acgaactggc gcatccccgg gcccgagctg aagttcgagg agcggtggat
     5101 gccgccgacc cacgtcgaga cgcccgatgc gccctgcaag gacatgtcga tcaccgtggg
     5161 gtggaacacc gcgcaggccg tcggaatcac gcgcgaggag atggacgcct gggcggcacg
     5221 ctcgcaccag cgtgcgatcg ccgccatcga cgcgggcaag ttcgttgacg agatcatccc
     5281 gctgaaggtt cagcagttcg acggatcggt ggtcgacttc agcgtcgacg agcatccgcg
     5341 ccgcgacacc accgtcgaga agctggctgc gttgaagccc ttgcatcccg agatcgaagg
     5401 gttttcgatc acggcgggca acagcagcgg c
//
LOCUS       DASRJA010000799         9082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_268152,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000799 DASRJA010000000
VERSION     DASRJA010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9082
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268152"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..894
                     /gene="truA"
                     /locus_tag="VFC01_36070"
     CDS             25..894
                     /gene="truA"
                     /locus_tag="VFC01_36070"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZN85076.1"
                     /translation="MNEPAIDSGGGFVRLRLDIAYDGTAFAGWALQAGQRTVAGVLDD
                     ALSTVFRVPVQLRAAGRTDAGVHATGQVAHVDIPIDTLPNAYPRATRAGDREFLPLVR
                     RLARFLPADVRILDIDRAPAGFDARFSALRRHYVYRLSTAPYGVQPQEARFVTAWPRP
                     LDVEAMAVASRNLVGLHDFAAFCRQREGATTIRDLQRLDWSRDGDRVTAYVTADAFCW
                     SMVRSLVGALLAVGEHRRETGWCATLLTSTQRSSDFAAAPPHGLTLVGVDYPPDDQLE
                     ARAKVTRDLRVVD"
     gene            complement(891..1478)
                     /locus_tag="VFC01_36075"
     CDS             complement(891..1478)
                     /locus_tag="VFC01_36075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN85077.1"
                     /translation="MATTTAAVVAVLVTPGLLPRAAADCPDVEVVFGRGQGEPPGLGA
                     VGDAFVAALQAKSGRPIASYAVNYPAANLDIAPGVADMAGHIESMAANCPNTRFILGG
                     YSLGGAVTFQVLNNGLSPGIDQRVIGVATFGNAARALNLPTPVPPQYAGKTLDQCNPG
                     DPACSGVPFWPAHLQPFYIMGGPVETAAGAAASLL"
     gene            complement(1576..2271)
                     /locus_tag="VFC01_36080"
     CDS             complement(1576..2271)
                     /locus_tag="VFC01_36080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN85078.1"
                     /translation="MRSDLDSRGWVRRGLLVVGALVLAAAGLIAPTVAPATGVLPSAS
                     AACPAVEVVFARGRLESPGVGVLGNAFINALRSKANKNIGVYAVRYPADNQIDVGAND
                     MSAHIQSMINDCPDTRLVVGGYSLGAAVTDVVLALPFDFFGFKNPLPPGADTHIAAVA
                     VFGNGIAWVGPISNFNPIYRERTIELCHGADPVCNPADPDTWQDNWPAHLANAYISAG
                     MANQAADFVAGRL"
     gene            complement(2329..2904)
                     /locus_tag="VFC01_36085"
     CDS             complement(2329..2904)
                     /locus_tag="VFC01_36085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN85079.1"
                     /translation="MPIGTASADDCPDIQVVFARGTNDAPGLGNVGGAFVDSLRSKVG
                     GRSVGVYAVNYPASFDFLAAAGGANDASGHIQYMVNNCPNTRLVLGGYSQGAAVIDVI
                     SAVPIPAVGFDNPLPPNVPDHVAAIAVFGNPSAKLGLPLTVSPVWGGRAIDLCNGGDP
                     ICQTDGESIAAHRAYPGGPTNQAANFVAGLL"
     gene            complement(3022..3690)
                     /locus_tag="VFC01_36090"
     CDS             complement(3022..3690)
                     /locus_tag="VFC01_36090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HZN85080.1"
                     /translation="MGGVTLLRMSGRLAAIVSTLVFAAVPLAVAPSAAADPCPDIEVV
                     FARGTNDAPGLGNPGQAFADALRSQVDGRTLTTYAVNYPANYDFLAAADGASDAANRI
                     SQMAVACPSTRIVLGGYSQGAAVVDMLAGIPPLGNKIGEVGSAPPLASSLVPNVAAVA
                     VFGNPATKFGNPITSSVFGGRSIDLCKDGDPICSRGRNPFAHSDYVSSGLTNQAANYV
                     AGII"
     gene            complement(3706..4389)
                     /locus_tag="VFC01_36095"
                     /pseudo
     CDS             complement(3706..4389)
                     /locus_tag="VFC01_36095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886937.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            complement(4413..4610)
                     /locus_tag="VFC01_36100"
     CDS             complement(4413..4610)
                     /locus_tag="VFC01_36100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HZN85081.1"
                     /translation="MFRVLIAAAFVCLAAAIGAAPTASASGPYANCTEAHQDGRYDIP
                     QGDPDYWNGGDRDHDGIACES"
     gene            4777..6042
                     /locus_tag="VFC01_36105"
     CDS             4777..6042
                     /locus_tag="VFC01_36105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZN85082.1"
                     /translation="MGVSKTSGGRASARLSSSLRAVHELFVAGQVDLDSTPLRPVVAD
                     SWRRSLATGVDPDLAGAMPAATIALDRMRASHPLAPALPVIRRLLVDDATDAGVVVAV
                     TAADGTLLWVEGDRNACRKAERMNFVPGADWSERGAGTNAPGTALALDAELQIRGTEH
                     FSRIVQPWSCTAVPVHDPATGVLIGAIDLTGGTQVASPQTLALVRATAVAVENHLALL
                     QLNQPTVKAPTTARLTVLGADRPRWQSELQTSTLTGRHADILVLLGRHPEGLSADHLA
                     MLLDDKDLDVVTIRAEMSRLRRVIGADYIDSRPYRLLKPIASDVSDVSDALQAGDVDR
                     ALRLYTGELLPQSVSPGIARLRRELSASMRGAVLSSGNLALLRRWLDLPEGRDDRDCW
                     RVLYNHAAPGSVMRAQARGRLAGLDFDLA"
     gene            6144..7667
                     /gene="adh"
                     /locus_tag="VFC01_36110"
     CDS             6144..7667
                     /gene="adh"
                     /locus_tag="VFC01_36110"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_214972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZN85083.1"
                     /translation="MTVYARPGAEGSVMSFQSRYENYIGGEWVAPAAGRYFENASPVN
                     GQPFCEIPRSDETDIEKALDAAHAAAPAWGKTTAAERAVILNKIADRIEENLESIAVA
                     ESWDNGKPVRETLNADIPLAVDHFRYFAGAIRAQEGSLSQIDDETVAYHFHEPLGVVG
                     QIIPWNFPILMATWKLAPALAAGNAVVLKPAEQTPASVLYLFSLIGDLLPPGVVNIVN
                     GFGLEAGKPLASSKRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSPNIFFNDV
                     LAEHDDYQDKALEGFTMFALNQGEVCTCPSRSLIQADIYDEFLELAAIRTKAVRQGDP
                     LDTETMIGAQASEDQLEKILSYIDIGKRDGAKLITGGERAELGGDLGGGYYVTPTVFT
                     GNNKMRIFQEEVFGPVVAVTSFTDYDDAISIANDTLYGLGAGVWSRDGNVAYRAGRDI
                     KAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLDHYQQTKNLLVSYGNKAQGFF"
     gene            7720..8781
                     /gene="adhP"
                     /locus_tag="VFC01_36115"
     CDS             7720..8781
                     /gene="adhP"
                     /locus_tag="VFC01_36115"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012361064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HZN85084.1"
                     /translation="MTQTLGAPVAPDTNDTLRAAVVTEFGAPLQVEELELPTPGYGEA
                     LVKLETSGVCHTDLHAAHGDWPVKPQPPFIPGHEGYGTVVALGEGVDDLKVGDKVGNA
                     WLWSACGTCEYCRTGWETLCESQKNGGYSVDGSFGTYMLVNAAYAARIPENADPLEVA
                     PILCAGVTVYKGLKVTDTRPGQWVAISGIGGLGHVAVQYARAMGLRVVAIDIDDSKLA
                     LATRLGAEVAVNAKTSDVVAEVQKATGGVHGVLVTAVHPQAFGQAIGLARRGGTIVFN
                     GLPPGDFPAPIFDIVLKGLTIRGSIVGTRQDMAEALDFYARGLIHPTVESASLDDINE
                     VFGRMERGQIDGRVVIDYR"
BASE COUNT         1577 a         3030 c         3028 g         1447 t
ORIGIN      
        1 ccgacgacgc caagtagcga agctgtgaac gaacccgcca tcgattccgg tggcgggttt
       61 gttcgtcttc ggctcgatat cgcttacgac ggaacggcat tcgcgggctg ggccctgcag
      121 gccgggcagc gcacggtggc aggcgttctg gacgacgcgc tgtcgacggt gttccgggta
      181 cccgtgcagt tgcgtgcggc gggccggacc gacgccggcg tgcacgcaac gggtcaggtg
      241 gcccacgtcg acattccaat tgatacgctg cccaacgcct atccgcgtgc gacgcgtgct
      301 ggcgatcgcg agttcctgcc gctcgtgcga cgtctcgcgc ggttcctgcc tgccgatgtg
      361 cgcatcctcg acatcgaccg tgccccagcc ggtttcgacg cccgattttc ggcgctgcgg
      421 cgacactacg tgtaccggct gtcgacggcc ccgtatggtg tccagccgca ggaggcgcgc
      481 ttcgtaacgg cctggccgag accactcgac gtcgaggcga tggcggtggc gtcgcgaaac
      541 ttggtggggc tgcacgactt cgccgcgttc tgccgtcagc gcgagggtgc caccacgatc
      601 cgcgatctac agcggctgga ctggtcccgc gacggagacc gggtcaccgc ctacgtcacc
      661 gccgacgcgt tctgctggtc gatggtgcgc tcattggtcg gagccttgct ggcggtcggc
      721 gagcaccgcc gcgaaaccgg ttggtgcgcc acgctgttga cgtcgacaca gcgctccagc
      781 gatttcgccg ccgcgccgcc gcacggcctg accctcgtgg gcgtcgacta cccgcccgat
      841 gaccagctcg aggcccgcgc caaggtcacc cgcgacctgc gggtcgttga ctaaagcagc
      901 gaggccgccg cgccggctgc tgtttcgacg ggcccgccca tgatgtagaa gggctgaagg
      961 tgtgcgggcc agaagggtac ccccgagcag gcgggatcac ccggattgca ttggtccagg
     1021 gtcttgcccg cgtactgagg cggaacgggg gtgggcagat tgagcgctcg cgcggcattg
     1081 ccgaacgtcg ccaccccgat cacacgctgg tcaatacccg gtgatagccc gttgttgaga
     1141 acctggaagg tcacggcgcc gccgagcgaa tacccgccga ggatgaacct ggtgttcggg
     1201 caattcgcgg ccatcgattc gatgtggccg gccatgtctg cgacaccagg agcgatatcc
     1261 aggttcgccg cgggatagtt gaccgcatac gacgcgatcg gcctgcctga cttggcctgc
     1321 agcgccgcga cgaaggcgtc cccgaccgca cccagaccgg gcggctcgcc ttgtccgcgg
     1381 ccgaatacca cctcgacgtc cgggcagtcg gccgccgccc ggggaagcaa gccgggagtg
     1441 acgaggaccg ccacgacggc agcggtggtc gtagccaaaa cggttatcca ccgcatcgga
     1501 cgggacatct ggactcggcc agggcggaca actcattacg ccaggttacc gtgcgtgtcg
     1561 cgctgcatct ggcgactaga gcctgccggc gacgaaatcg gctgcctggt tggccatgcc
     1621 ggcgctgatg tacgcattgg cgaggtgggc gggccagttg tcctgccatg tgtcgggatc
     1681 ggccggatta cagacgggat ccgcgccgtg acacaactcg atggtcctct cgcggtagat
     1741 cgggttgaag ttcgatatcg gcccgaccca agcgattccg ttgccgaaca ccgcgaccgc
     1801 ggcgatatgt gtgtcagcgc cgggcggcag tgggttcttg aatccgaaga agtcgaaggg
     1861 cagtgcaagg accacgtcgg tgaccgccgc gccgagcgag tagccgccca ccaccagccg
     1921 ggtatccggg cagtcgttga tcatcgattg gatatgcgcg ctcatgtcgt tggcgccgac
     1981 gtcgatctga ttgtcagcgg ggtagcgcac cgcgtagacg ccgatgttct tgttcgcctt
     2041 cgagcgcagc gcgttgatga acgcgttccc gaggacacca acgcccggcg actcgaggcg
     2101 gccacgggca aatacgacct cgaccgcggg gcacgcagcg gacgccgacg ggaggacacc
     2161 cgtcgcaggg gcgacggtcg gcgcaatcag accggcagcg gccaggacta gtgccccgac
     2221 aaccaataaa cctctgcgga cccaacctcg tgagtcaaga tcactgcgca cttcgccgat
     2281 cctaacggaa tgctgtgaac gcagccgtca attccccttt gccgcgggct agagcagacc
     2341 cgccacgaaa ttcgccgcct ggttggtcgg tccacccggg taggcgcgat gcgcggcgat
     2401 gctttcgccg tcggtctggc atatcgggtc accgccattg cacaagtcga tggccctgcc
     2461 accccatacc gggctcacgg tgagtgggag gcccagtttg gcggacgggt tgccgaacac
     2521 ggcgatggcg gcgacgtgat cgggcacgtt gggtggcagc gggttgtcga acccgacggc
     2581 ggggatcggc accgcgctga tgacatcgat caccgccgcg ccctgcgagt acccgcccag
     2641 taccagcctg gtgttggggc agttgttgac catgtactgg atatgcccgc tggcgtcgtt
     2701 ggccccgcct gctgcagcca ggaagtcgaa gctggcgggg taattcaccg cgtacacgcc
     2761 gacggatcgg ccgccgacct tgctgcgcag cgaatcgacg aacgcgcctc cgacgtttcc
     2821 taggcctggc gcgtcattgg tgccccgcgc gaagaccacc tggatgtcgg ggcagtcgtc
     2881 ggctgacgcg gtcccaattg gcatgagaga caccgtcggt gcgagaagag ccgcggaggc
     2941 cgccaggaca gcagttgccg aggcgttgag ccagcggcga attcgtttcg atggcacaag
     3001 aaattctagc gcatagacag gctaaattat gccagctacg tagttcgcgg cctgatttgt
     3061 caggcccgag ctcacgtaat cgctgtgagc gaacgggttg cggccgcggg agcagatcgg
     3121 gtcaccgtcc ttgcagaggt cgatcgacct gccgccgaat accgatgagg tgatcgggtt
     3181 cccgaacttc gtcgcggggt tcccgaacac cgcgaccgct gcgacattgg ggacgaggct
     3241 gctcgccagc ggtggtgccg acccgacctc gccgatcttg ttgccgagcg gcgggatgcc
     3301 ggccagcatg tcgaccaccg cggcaccctg cgaatatccg ccgaggacga tccgtgtcga
     3361 cgggcaggcg accgccattt gggagatgcg gttggccgcg tcggaggccc cgtcggcagc
     3421 ggccaggaag tcgtagttag cgggatagtt gaccgcatat gtggtcaggg tgcggccatc
     3481 aacctgcgac cgcagcgcgt cggcgaaggc ctggccgggg tttccgagcc cgggcgcgtc
     3541 gttggtgccg cgggcgaaga cgacctcgat gtcggggcac ggatccgccg ccgccgacgg
     3601 ggcgacagcg agcggcacgg ccgcgaagac cagggtcgaa acgatggcgg ccagccggcc
     3661 tgacatccgg agcaaagtca cgccgcccat cttcacatat ctggatcagc tccgaccaac
     3721 cgattggtcg tatgccacgc ctccgggtgg aacttccgcc cacgtcaatt cggccagctt
     3781 ctcggtgcga tcgccctgtc gggcgagcgc gagtccgacg agcacgacga cgccgccgat
     3841 cgcctgcgtc ggcgtgatgg cctcgcccag caacacccac gcggccagca cagcgaacat
     3901 cacctccgac aggccgacaa gggaggcgaa ccgcggctgt agccgggcga cgcccatgat
     3961 gccgagcgtg taggcgatcg cggtggggat cagagccagt gcgatgaccg gcaccagcca
     4021 cgacgtcgtc caccccgcga cgacggcatc gttggtggtg aaggtcaggg gcatcagccc
     4081 ggtggcgccc agggtcgcga ccgcggtcgc gccgacgatc aggcccgccg ttgccagtgt
     4141 gaccgcgtct aggccgtccc cgtcggtagc gacggaggag gacatcacga agtagcaggc
     4201 ggcgcacacg gcggccgcca gtgcccaacc gacgccgatc aggttgatgt gggcaccggc
     4261 aaagacgttg agcaccaaca tgattcccgc tatcgccaat gcgacaccga gaagcgtcag
     4321 gccgctcggc cggcgtcgcg acgtggccca cacccagccg actacgagaa tgggggcggt
     4381 gtactccagc cgggtgggac cgcacagtcc tgttatgact cgcatgcgat gccgtcgtgg
     4441 tcgcggtcgc cgccgttcca atagtccggg tcgccctgcg ggatgtcgta gcggccatcc
     4501 tggtgagctt ccgtgcagtt tgcgtatggt ccgctggcgc tagccgtcgg agccgcgcca
     4561 atggcggcgg ccaagcaaac gaatgctgcg gcaatcagaa cacgaaacat gttcactcca
     4621 ttgtttgcgg tcgccgattt ctgcaccagg gttgtgatcg agcgcgcggc cacagccctc
     4681 aagtcgaaat cgcgcgggga gggccaaaag tccctgccgc gaacccgcga aagtgggcta
     4741 tcgtggcggc cacgcgtgcg aggagcgtga tcgccggtgg gagtctcgaa gacatcgggt
     4801 ggacgggcgt cggcccggct gtcgtcgtcg ctgcgcgccg tccatgagct cttcgtcgcc
     4861 ggacaggttg atctggactc cacgccgttg cgtcctgtcg tagccgacag ctggcgccgc
     4921 agcctggcaa ccggcgtcga ccccgacctg gctggcgcga tgcccgccgc gaccattgct
     4981 ctggaccgca tgcgcgcctc ccatccactc gcgccggccc tccccgtcat ccgccgcttg
     5041 ctcgtcgacg acgcgaccga cgccggcgtc gtcgtcgccg tgaccgcggc cgacggcaca
     5101 ttgctctggg tcgagggcga tcgcaacgcg tgccgcaagg ccgaacggat gaacttcgtg
     5161 cccggtgccg actggagtga gcgcggcgcc ggtaccaatg caccgggcac cgcgctcgcc
     5221 ctcgatgccg agctgcagat ccgcggaacc gagcacttct cccggatcgt ccagccatgg
     5281 agctgcaccg cggtgccggt gcacgacccc gccaccggag tgctgatcgg cgccatcgac
     5341 ctgaccggtg gtacccaggt cgcgtcgccg cagaccctcg ccctggtccg cgcgaccgcc
     5401 gtcgccgttg agaaccacct cgccctgttg cagctcaacc agcccaccgt caaagcgccg
     5461 acgaccgcac ggctgacggt gctcggcgcc gaccgcccgc gctggcagtc cgagcttcag
     5521 accagcacat tgaccggccg acacgccgac atcctggtcc tgctcggccg ccaccccgaa
     5581 ggtctgagcg ccgaccatct cgcgatgctg ctcgacgaca aggacctcga cgtggtgacg
     5641 atccgcgccg agatgtcccg gctgcgccgc gtgatcggcg ccgactacat cgactcccgg
     5701 ccgtaccgct tgctcaaacc gatcgccagt gacgtgagcg acgtgtccga tgccctgcag
     5761 gccggcgacg tcgaccgcgc gctgaggctc tacaccggcg agctgctccc gcaatcggtg
     5821 tcacccggaa tcgcgcggct ccgtagggaa ctcagcgcga gcatgcgcgg cgccgtgctg
     5881 tcgtcgggaa accttgcgct gcttcgccgt tggctggatc tgccggaggg ccgcgacgac
     5941 cgcgactgct ggcgggtgct ctacaaccac gctgccccag gctcggtcat gcgtgcccag
     6001 gcccgcggcc gccttgcggg gctggatttc gacctggcct aaggccccgg cggttgcaac
     6061 gtcgtgcaac cgtgctgcaa cggtccaaaa cctaccttca gtgacgtccg acacatttgc
     6121 gtcgcgaagg caggagaaac gccatgaccg tatatgcccg accgggtgcc gaaggctcag
     6181 tgatgtcctt ccagtctcgg tacgagaact acatcggcgg cgagtgggtt gcgcccgccg
     6241 ccggccggta cttcgagaac gccagcccgg taaacggtca gccgttctgc gaaatccctc
     6301 gctctgatga gaccgacatc gaaaaggcgc tcgacgccgc ccatgcggcc gcaccggcgt
     6361 ggggcaagac gaccgccgcc gagcgcgcgg tgatcctcaa caagatcgcc gaccgcatcg
     6421 aggagaacct cgaatccatc gcagtcgccg aatcctggga caacggcaag ccggtgcggg
     6481 agacgctgaa cgccgacatc ccgttggcgg tcgatcattt ccggtacttc gccggtgcga
     6541 tccgcgcaca ggaggggtcg ctgtcgcaga tcgacgacga aaccgtcgcc tatcacttcc
     6601 acgaaccact cggcgtggtg ggccagatca tcccgtggaa cttcccgatc ctgatggcca
     6661 cctggaaact ggcgccggcg ctcgcggccg gaaatgcggt ggtgctcaag cccgccgagc
     6721 agacgcccgc gtccgtgctc tacctcttct cgttgatcgg tgatctgctg ccgcccggcg
     6781 tggtgaacat cgtcaacggg ttcggccttg aagcgggtaa gccgctggcg tcctcgaagc
     6841 gaatcgcgaa gatcgcgttc accggtgaga ccaccaccgg ccggttgatc atgcagtacg
     6901 cgtcgcaaaa cctcatcccg gtgacgctgg agctgggcgg caagagcccg aacatcttct
     6961 tcaacgatgt cctggccgag cacgacgact accaggacaa ggcgctcgaa ggctttacga
     7021 tgttcgccct caaccagggc gaggtgtgca cctgcccgtc gcgcagcctg attcaggccg
     7081 acatctacga cgagttcctc gagctggccg cgatccgcac caaggccgtc cgtcagggcg
     7141 acccgctgga caccgagacg atgatcggtg cccaggcatc cgaggaccag ctggaaaaga
     7201 tcctgtcgta catcgacatc ggcaagaggg atggcgcaaa gctcatcacc ggcggtgaac
     7261 gcgccgagct cggcggcgat ctgggcggcg gatactacgt caccccaacg gttttcaccg
     7321 gcaacaacaa gatgcggatc ttccaagagg aggtcttcgg gccggtggtc gcggtgacgt
     7381 cgttcaccga ttacgacgac gcgatcagca tcgccaacga cacgctctac ggcctcggcg
     7441 cgggggtgtg gagtcgcgac ggcaatgtcg cctaccgggc gggccgggac atcaaggccg
     7501 gccgggtgtg gacgaactgc taccacgcct atcccgcgca tgctgcgttc ggcggctaca
     7561 agcagtcggg catcggacgt gagacccaca agatgatgct cgaccactac cagcagacca
     7621 agaacctgct ggtgtcctac ggcaataagg cccaaggctt cttctgagcc gggaccaaag
     7681 acccttcaac aaccacactt tcacgattgg aatgatgaaa tgacccagac gcttggcgcc
     7741 cctgtcgcgc ccgataccaa cgacaccctg cgtgccgccg tcgtcaccga attcggcgca
     7801 cccctccagg tcgaagagct cgaactgcca acgccgggct acggcgaggc attggtcaag
     7861 ctcgagactt ccggagtgtg ccacacggat ctacatgccg cacacggaga ttggcccgtc
     7921 aagccacaac cgccgttcat ccccggacac gagggctacg gcaccgtcgt ggcactcggt
     7981 gagggcgtcg acgacctgaa ggtcggagac aaggtcggca acgcctggct gtggtcggcc
     8041 tgcggcacct gcgagtactg ccgcaccggc tgggaaacgc tgtgcgagag ccagaagaac
     8101 ggcggttaca gcgtcgacgg cagcttcggt acctacatgc tggtgaacgc cgcctacgcg
     8161 gcgcgcatcc cggagaacgc cgatccgctg gaggtcgcgc cgatcctgtg cgcgggcgtc
     8221 actgtgtaca agggcctcaa ggtgaccgac acccggcccg gccagtgggt cgcgatctcc
     8281 ggcatcggcg ggctcggcca cgttgcggtg cagtacgcgc gcgcgatggg actgcgggtg
     8341 gtggcgatcg acatcgacga ctccaagctc gcgctggcca cgcgccttgg cgcggaggtc
     8401 gccgtgaacg cgaaaacgtc cgacgtggtc gccgaggtgc agaaggccac cggcggtgtg
     8461 cacggggtgc tcgtcacagc cgtgcacccg caggccttcg gccaggcgat cggcctggcg
     8521 cggcgcggcg ggacgattgt gttcaacggc ctaccgccag gcgacttccc ggcgccgatc
     8581 ttcgacatcg tgctcaaggg tttgacgatc cgcgggtcga tcgtcggcac tcgccaggat
     8641 atggccgagg cgctggactt ctacgcccgc ggtttgatcc atccgacggt cgagagtgcg
     8701 agcctggacg acatcaacga ggtgttcggc cggatggaac gcggccagat cgacgggcgc
     8761 gtcgtcatcg actaccgcta gtcatcggcg tagcagggcc ccggttgacg ctggggccct
     8821 gttcgctgca gacagttggg agcgaatacc gcaaggcgac gtcgtcgcgt agcgcggcgg
     8881 tgaaacccag gtagtccatt acgctgtcgg cccctggggc gcggcgggaa tctaacgccg
     8941 tgaccgagag ctgcacactg gccgcaccgg cagttcacct gccgccgtgt gggccgttcg
     9001 tgcagcgaat cctcaagcat ccccgcgtga tgcactacaa ccgactcgtc gcgctggtgc
     9061 tcgtcgggaa catcgcgctc gc
//
LOCUS       DASRJA010000800         4892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_1546807,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000800 DASRJA010000000
VERSION     DASRJA010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546807"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VFC01_36120"
     CDS             <1..882
                     /locus_tag="VFC01_36120"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZN85085.1"
                     /translation="EEHRRDLAEVGVPVADSGATLFGGLTTVQQVAEMAAAPVQHVDP
                     AIAVTGVHWPATTANGGLVLHDEAKKTLAALGVSFVSEQVARTLDDVAEAALGAGPFA
                     LKLLDRNLPHKAAAGGVELGVATVADARLVAARLFERASSADARILLQVMTPSVCEIF
                     LGTVDDPVAGPVVVVGRGGRDVETDPDVAVELAPVDSTQVRNMVAGIRPLMAELRLAA
                     QRTGRDVDQLVSDIAEVAASVSALAASESGRVQSIDINPLVITNAGELKALDVRVELS
                     SESSGTDRAHAHTEGTR"
     gene            879..1673
                     /locus_tag="VFC01_36125"
     CDS             879..1673
                     /locus_tag="VFC01_36125"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZN85086.1"
                     /translation="MSTNQSTVLYEVKDKIATITLNRPERLNAMNVAMVDRLPEVWRQ
                     FENDDDAHVAIVRGAGDKAFCAGGDLKEMSEGSVQHIYDSTETFAGVRYPSATYQFET
                     RKPVIGAIHGYALAGGFTLALNCDIRIASEDAKFGVAEVKVGRGAPWAVPLLWAVPAG
                     IALELIMRGNQIDARRAYEVGFVNHVVPRAQLYDTAWAIAEEIRDNAPITVRAHKQLL
                     YRAMDAGRALGFLLADELCRAIYTSEDCQEGQRAFREGRKPVFRGR"
     gene            1676..2659
                     /locus_tag="VFC01_36130"
     CDS             1676..2659
                     /locus_tag="VFC01_36130"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85087.1"
                     /translation="MATPTITEHSAVGHWSTGRSGTRVQLTETLLRDGAQAMPEEALQ
                     YFSVDRKMSVINELIALGMPGIEVASFAHPRVLPQFADAEEIISRLPKDTSITFRAVT
                     PNLRGLQRASRAGAPGLVTMIGMLSCSEEYQRRNVQKSIEDGLDELAKMLDYSRAEGI
                     RLIAGAGVAFECPYEGHIDPARVVSIVNRLASIGFDEVWMGDTFGMASAPSVYALLAE
                     LRSQQPNMTFGVHLHNRHGLALANVLAAVDAGVTMFDTSLLGIGAGSVIPGNRTEMGN
                     VATEEVAHLAESLGWATGIDLVGLRELGLQLAGEAGLDIRSRVLRTGISTA"
     gene            2709..3821
                     /locus_tag="VFC01_36135"
     CDS             2709..3821
                     /locus_tag="VFC01_36135"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN85088.1"
                     /translation="MLNGKFVVDATVHPFDYSEENWGDGYKQVIGGYHHHRLYNKVVE
                     SSRPDYLLRQNEFVSDFDPYAMTHALFAESVVDFAIIHALPNLGFTKGVVTDLDKMVK
                     LRERFSDRFKLYGWVTTLDLDEAIKHLEWQVREAHIDGLKCFPSLVYKGKHTGWRMDG
                     PEFAVPLFEAARDLGIKNIAIHKALPMFGPSPMSFFKVDDLDAVLPKFPDINFFMIHA
                     GMNFLEETCVMLHRFPNFHANLESTFANAVERPRIFAETLGQMLYHGTPEQIVFASGC
                     NLLHPQPALEAFESFQMPRDLVEGFGFPEVTDEIKAKILGLNIARTHGIDPQAVLAAV
                     KDDEFERAKADGLAAPWSALRRDEADNRVAASAGAL"
     gene            3821..4156
                     /locus_tag="VFC01_36140"
     CDS             3821..4156
                     /locus_tag="VFC01_36140"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HZN85089.1"
                     /translation="MPDTQLDTATVEKALQGVIDPCSKAMGQPMDIVTMGLVERIDIA
                     DGKVTITVILTDPNCFFFSQIEQFVGDVLAELPGVDEVDVQISSTTLWSSDRIRTRLP
                     VTVSPGHYA"
     gene            4287..>4892
                     /locus_tag="VFC01_36145"
     CDS             4287..>4892
                     /locus_tag="VFC01_36145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884340.1"
                     /note="catalyzes the ferredoxin-dependent oxidative
                     decarboxylation of arylpyruvates; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid ferredoxin oxidoreductase"
                     /protein_id="HZN85090.1"
                     /translation="MTPSTAAMGAADQSFRVGRYRDDEGVALLSGVQALVRLPLLQMR
                     LDRAAGVHTACLITGYEGSPLAGYDLELGRIQGLLDEHDVVFQPAVNEELAATAVGGS
                     QLATARPNARYDGVLGLWYGKAPGLDRASDAIRHGNLQGADYRGGALLIVGDDPATKS
                     SSLPSASELALADLAVPVLYPGSVAEVLEFGLYAIALSRLTG"
BASE COUNT          893 a         1519 c         1622 g          858 t
ORIGIN      
        1 gaggagcacc gccgcgacct cgctgaggtc ggagttccgg tcgccgacag cggcgctacc
       61 ctcttcggcg gcctcaccac ggtgcagcag gtcgccgaga tggccgcggc gccggtgcaa
      121 cacgtcgacc ccgcgatcgc ggtgacgggt gtgcattggc ctgccacaac ggcgaacggg
      181 ggcctcgtct tgcacgacga ggcaaagaag acgttggccg ccttgggtgt gtcgtttgtg
      241 tccgagcaag tggctcgaac tctcgatgac gtcgcggagg ctgccttggg tgccgggccg
      301 ttcgcgctca agctgctcga ccgaaacctt ccacacaagg cggccgcggg tggcgtcgaa
      361 ctcggcgtgg caactgtcgc cgacgcgcgg ctcgtggccg cgcggctgtt cgaacgcgcc
      421 agctcggccg acgcccgaat tctcctgcag gtgatgacgc catcggtttg cgagatcttc
      481 ttgggcaccg tcgacgaccc cgtcgctggc ccggtcgtcg tcgtcggccg gggcggccgg
      541 gatgttgaga ccgacccgga cgtcgcagtc gagctcgcgc cggttgactc gacgcaggtg
      601 cggaatatgg tggccgggat ccggccgttg atggccgagt tgaggcttgc agcgcaacgc
      661 accggacgcg acgtggacca gctcgtatcc gacatcgcgg aggttgccgc ctccgtctcc
      721 gcgctggctg cgtcagagag cggccgagtc caatcaattg acatcaaccc gctggtgatc
      781 acgaatgcgg gggagctgaa agcactcgac gttcgtgtcg agctgtcatc ggagtcgtcg
      841 ggcaccgatc gtgcccacgc gcacacggaa ggtacacggt gagcacgaac caaagcaccg
      901 tcctctatga ggtcaaagac aagatcgcaa cgatcacgct gaaccggcct gagcgcctga
      961 acgcgatgaa cgtggcgatg gttgaccggc tacccgaggt gtggcgtcag ttcgaaaacg
     1021 atgacgacgc gcacgtcgcc atcgtccgcg gcgccggcga taaggccttc tgcgccgggg
     1081 gcgatcttaa ggaaatgtcc gagggctccg tccagcacat ttatgacagt acggagacgt
     1141 ttgcgggtgt gcggtatccg agcgctacct accagttcga gacccgaaaa cccgtcatcg
     1201 gcgctattca tggatatgcc ctcgccggcg gcttcaccct ggcgctgaac tgcgacatcc
     1261 ggatcgcctc tgaggatgcc aagttcgggg tggcggaggt gaaggtcggc cgtggcgcgc
     1321 cgtgggcggt gccactgcta tgggcagttc cagcgggcat cgcgctcgaa ttgatcatgc
     1381 gcggcaatca gatcgacgcc cggcgcgctt acgaggtcgg atttgtcaat catgttgtgc
     1441 cccgggctca gctctacgac accgcgtggg ctatcgccga ggagatccgc gacaacgcgc
     1501 cgatcactgt tcgggcccat aagcaactgt tgtatcgcgc catggacgca gggcgtgctc
     1561 tcgggttcct gctcgccgac gaattgtgcc gcgcgatcta tacgagcgag gactgccagg
     1621 aaggccagcg ggcattccgc gaaggccgca agcccgtgtt caggggacgg tgacgatggc
     1681 gacgccaacg atcaccgagc actccgccgt cgggcactgg agcaccggga ggtccgggac
     1741 ccgggttcag ctgaccgaga cgttgctgcg ggacggcgcc caggcgatgc cagaggaagc
     1801 gctgcagtac ttctcggtcg accgaaaaat gagcgtgatc aacgagctga ttgcgctcgg
     1861 gatgcccggc atcgaggttg ccagcttcgc ccacccgcgg gtgctgccgc agttcgccga
     1921 cgctgaggag atcatctcca gactgcccaa ggacacctcg atcacgttcc gggccgtcac
     1981 gccgaacctt cgcggactcc aacgtgcctc gcgcgcggga gccccggggt tggtgacgat
     2041 gatcggcatg ctgtcgtgca gcgaggaata ccagcgtcgc aacgtgcaaa agtcaatcga
     2101 ggatgggctc gacgagctcg ccaagatgct ggattactcc cgcgccgagg ggatacggct
     2161 gatcgcgggt gccggggttg ccttcgagtg tccgtacgaa gggcacatcg atccggctcg
     2221 ggtggtgtcg atcgtgaacc gactggcatc cattggcttc gacgaggtgt ggatgggcga
     2281 caccttcgga atggccagcg cgcccagtgt ctacgcactg ctcgctgaat tgcgctcgca
     2341 gcagccgaat atgaccttcg gtgtgcacct gcacaaccga cacggcctgg cactggcgaa
     2401 tgtattggcc gccgtggatg cgggcgtgac gatgttcgac acctcgttgc tcggcatcgg
     2461 cgccggcagc gtgattcccg gtaaccgcac cgagatgggg aatgtggcga ccgaggaggt
     2521 cgcgcacctc gcggagagcc tgggctgggc caccgggatc gatctcgtcg ggctgcgtga
     2581 gcttggcttg cagctggccg gcgaggccgg attggacatt cgcagcaggg tgctgcggac
     2641 ggggatcagc acagcatagc gagagctagc agtgccatca ctgaatcgat tacctcaaag
     2701 gaggagtagt gctcaacggc aagttcgtgg tggacgcgac agtgcatccc ttcgactaca
     2761 gcgaagagaa ctggggcgac ggctacaagc aggtgattgg ggggtatcac caccatcggc
     2821 tgtacaacaa ggtggtcgag tccagtcggc ccgactatct gctgcgacaa aacgagttcg
     2881 tcagcgactt cgacccctat gccatgacgc acgccctgtt cgcggagagc gtcgtggact
     2941 tcgccatcat ccacgctcta ccgaacctcg gtttcaccaa aggtgtggtc accgacctgg
     3001 acaagatggt caagctacgc gagcggtttt cagaccgatt caagctctat ggctgggtga
     3061 ccacgctcga cctggacgaa gcgatcaagc acctcgagtg gcaggtacgc gaggcccaca
     3121 tcgatggcct gaaatgcttc ccatcgctgg tctacaaggg caagcacacc gggtggcgga
     3181 tggacgggcc cgagttcgcg gtgccgctgt tcgaggcagc tcgtgacctc gggatcaaga
     3241 acatcgccat tcataaggct ctgccgatgt ttggtcccag cccgatgtcg ttcttcaaag
     3301 tcgatgacct cgacgctgtg ctgcccaagt tcccggacat caatttcttc atgatccacg
     3361 ccgggatgaa cttcctggaa gagacctgcg tgatgctgca tcggttcccg aatttccacg
     3421 ccaatctgga gtcgaccttc gctaatgccg tggaacggcc gcggatattt gccgagaccc
     3481 tcggtcagat gctttaccat ggaactccgg agcagatcgt gtttgccagc ggttgtaatc
     3541 tgctgcaccc gcagcctgcg ttggaggcgt tcgagtcgtt ccagatgccg agggatctgg
     3601 tcgagggttt cggcttcccc gaagtcaccg acgagatcaa ggcaaagatc ctcggcctca
     3661 acatcgccag gacgcatggc atcgacccgc aagccgtgct ggccgcggtg aaagacgacg
     3721 agtttgagcg ggcaaaggcc gacggcctgg ccgcgccctg gagtgcattg cgccgtgacg
     3781 aggccgacaa ccgggtggcg gcaagcgcgg gtgcactctg atgcccgaca cacagctcga
     3841 caccgcaact gtggagaagg cgctgcaggg ggtcatcgac ccgtgcagca aggcgatggg
     3901 ccagccgatg gacatcgtga ccatgggact ggtcgagcgc atcgatatcg cggacggcaa
     3961 ggtcacaatc acggtgatac tcacggaccc gaactgcttc ttcttcagcc agatcgagca
     4021 gtttgtcggc gacgtgctcg ctgagctgcc cggcgtcgat gaagttgacg tccagatctc
     4081 cagtacgacg ttgtggagct ccgaccgaat tcggactcgg cttccggtca ccgtgagccc
     4141 gggccactat gcctgacctc ggctgaaacc gggagccggt cgcgctggca accgcgcctg
     4201 gctcccggta ccggtgctgc gcgcactatt cccgccggac acccggcccc ccgtcgatac
     4261 gcgaagaccc gaaggagtgg acgaccatga cgccctcaac tgcagcgatg ggcgccgccg
     4321 accagtcgtt ccgagtcggg cggtaccgcg atgacgaagg cgtcgcgctg ctgtcgggcg
     4381 ttcaggctct ggtgcgtctt ccccttctgc agatgcggct ggaccgggcg gcgggggtac
     4441 acaccgcgtg tctgatcacc ggctatgaag gctctccact ggccggatac gacctggaac
     4501 tcggccgcat ccaaggactt ctcgacgaac atgacgtggt cttccagccc gcagtcaacg
     4561 aagagctcgc ggcgacggcg gtggggggca gccagctcgc caccgcccga ccgaacgcac
     4621 ggtacgacgg ggtgctcggc ctttggtatg gcaaggcgcc cggcctggac cgggccagcg
     4681 atgcgatccg gcacgggaac cttcagggcg ctgactaccg cggcggcgcg ctgctgattg
     4741 tgggagacga cccggccacc aagtcgtcca gcctgccgtc ggcatcggag ctagcgctgg
     4801 ccgacctcgc ggtgcccgtg ctttaccccg gcagtgtcgc ggaagtcctc gaattcgggc
     4861 tctacgcgat cgcactgtcc cggttgaccg gc
//
LOCUS       DASRJA010000801         4763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2350662,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000801 DASRJA010000000
VERSION     DASRJA010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2350662"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2362)
                     /locus_tag="VFC01_36150"
     CDS             complement(<1..2362)
                     /locus_tag="VFC01_36150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZN85091.1"
                     /translation="MRPSTGRFAVGGQRPATPPIRGRADELKVIGALVTALVQGHGGI
                     LIIEGPPGIGKSRLLTEVMALADKGGVRTLFGEAFEYQQTVPFFALFMATLRADPPVG
                     DADALRRLGGSADLRYWVVHDLADAIHAAAAETPLAIALENIHWADDSTLMALRSLAS
                     TGPDVPVLWVLTARTGAGGPAVQESLSVLQRADATFMRVAAMSPGAVADMVCDAVRAN
                     ADESLLKLATKAHGNPFLVSELVGGLDEEGRLNVSGGRAVASGIALPRRLGASMRQRL
                     DDLSAGAGEVVRVAAVLPDRFSAGLLAAMLERKPTSLMSALEEAVRADLLVEDGERLR
                     FRHDLLREATRQSLPQSLRRAMERQSASIMLSMGAAPAEVATQLARSAEPGDREAIGA
                     LRQAAQSVGHSDASAAADLSKRALELLPANDTDHGLLVAETVGLLNRASHYTEAEELA
                     VAVLSEAASPQEEAEIRLRLPTLTKHSAQRRVEEHRRALRLGDISEVTRARHLAWLAY
                     NLALHDQRGEARAAANEAAAAAASAGDLEATIVADVALALLDDGEGYAGRALDRLEEL
                     CNRARANDVTAAHFPAALNYAFLLAMVGRLDDAAAQVADGSQQARREGNAMALDTWAT
                     IDGVVHLTAGRLSAARAATESLPRPERTGVTPLDMIGIVILTEVAVRTDDRNLLQQMV
                     NDARDANSTGSSIVDRGTAYVLALAAWQRDDVHDAMRWLGNIALFGTPFFPQVLDQVI
                     LGARVASAAGDAGLRARVLRATELLEGERPEIPLFTGVAGYARGILE"
     gene            complement(2482..3501)
                     /locus_tag="VFC01_36155"
     CDS             complement(2482..3501)
                     /locus_tag="VFC01_36155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZN85092.1"
                     /translation="MTRANSVPAVELGDELSVSAIGFGAMVLTPVYGEVDDAESLATL
                     NHAVDLGVTFIDTANQYGGGANEQLISRLLADRRDEVTLATKFGIVGTPADRAAGKLV
                     ARGDAAYVHQSIDESLRRLQTDVVDLYYMHRRDVTVPIEETVGAMAELVAAGKVRHLG
                     LSEVTAEELRAAVAVHPIAAVESEWSIWSRDVERNVVPAAAELGVGFVPYSPLGRGFL
                     TGTIRSADDLSSTDFRRNLPRYAEGALDANLAVVEVVKSVADQQGATAAQVALAWLRY
                     RADALGVASVPIPGTRRAARVEENLGSLSVTLTPKQLDALDAAGSSVTGHRLKEMGWV
                     SGGRE"
     gene            complement(3625..3885)
                     /locus_tag="VFC01_36160"
     CDS             complement(3625..3885)
                     /locus_tag="VFC01_36160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85093.1"
                     /translation="MTEPVDVVGYTSGRSALSVTTSGSALSAAKAHPQRLWTRFATAS
                     DLALGVRGSILRSVVLSAVTSTPVGMRSKIEGAVDPLGYPHL"
     gene            3968..4288
                     /locus_tag="VFC01_36165"
     CDS             3968..4288
                     /locus_tag="VFC01_36165"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZN85094.1"
                     /translation="MTRHFPTMNTATTDINVLIIVGLRAASVDRELAKVAADSPADGI
                     ALNMFDSLTELPRYREPFENLETPSSVGDLRRAAAEADAVLIVTTYHGRVPAVVHNAI
                     DWLT"
BASE COUNT          851 a         1677 c         1440 g          795 t
ORIGIN      
        1 gttcgagtat cccgcgggca tacccggcca cccccgtgaa caacgggatc tccggccgct
       61 caccctcgag cagctcggtg gcccgcagga cacgtgcgcg caagccggca tcaccggccg
      121 ccgacgccac ccgcgcgccc aagatcacct gatctaaaac ctgggggaaa aatggtgtcc
      181 cgaaaagggc gatgttgccc agccagcgca tcgcgtcatg gacatcgtcg cgctgccacg
      241 ccgccagcgc cagcacgtac gccgttcccc gatctacgat agatgagcca gtggagttgg
      301 cgtcgcgcgc gtcattgacc atctgctgca acaagtttcg gtcatcggtg cgcactgcca
      361 cctcggtcag aatcacgatg ccgatcatgt ccagcggggt cacgccagtc cgctctgggc
      421 gcggcaggga ttcagtcgcg gcgcgggctg cagacaggcg gcccgcagtc aagtggacta
      481 caccgtcaat cgtggcccag gtatcaaggg ccatcgcgtt gccttcccgg cgggcttgct
      541 gtgatccgtc ggcgacctgg gccgcagcgt catccaatcg gccgaccatc gccagtagga
      601 acgcgtagtt gagtgcggcc gggaagtgag cagcggtcac atcattcgcg cgggctcggt
      661 tgcaaagctc ctcgaggcgg tctagggcgc ggccggcgta cccctcccca tcgtcgagca
      721 atgcgagggc gacatcggcc acgatggtgg cctcgagatc gccagccgat gcagcggccg
      781 cggcagcctc gttggctgcc gcgcgcgctt ccccacgttg gtcgtgcaac gccaggttgt
      841 aggccagcca cgccagatgc cgtgcccggg tgacctcgct gatatcgccc aagcgcagcg
      901 ctcggcgatg ctcctccacg cgccgctgtg cactgtgctt ggtgagcgtc gggaggcgca
      961 gccgaatctc ggcctcctct tggggtgacg ccgcctccga gagcaccgca accgccaact
     1021 cttcggcctc cgtatagtgg ctggcccggt tgagcagtcc caccgtttcg gcgaccagta
     1081 acccgtggtc agtgtcgttg gcgggcaata gctccaacgc gcgcttgctc aagtccgcgg
     1141 ccgcactcgc gtcactgtgg ccaactgatt gagcggcctg ccgcagcgcg ccgatcgctt
     1201 cccggtcccc cggctcggcg ctgcgcgcca gctgggtggc cacctcggcc ggggccgcgc
     1261 ccatactcag catgatcgac gcggattggc gttccatcgc gcgccgcagc gactgcggca
     1321 acgattgtcg ggtggcctca cgcaacaagt cgtgccgaaa cctcaaccgt tcaccgtctt
     1381 cgacgagcag atccgcgcgc actgcctcct cgagcgccga catcaacgac gtcggcttac
     1441 gttccagcat cgcggccaac agaccagccg aaaaacggtc cggcaacacg gcagccaccc
     1501 gcaccacctc gccagcaccg gcggagagat catcgagtcg ctgccgcata cttgcgccaa
     1561 ggcgtcgcgg cagcgcgatt ccggaggcca ccgcacgccc gccgctgacg ttgagccggc
     1621 cctcttcgtc cagacccccg accagctcgc tgacaaggaa tggatttccg tgggccttgg
     1681 tggccaactt cagtagcgac tcatcggcgt tcgcgcgcac tgcgtcgcag accatgtcgg
     1741 cgaccgcgcc cggcgacatt gccgccaccc gcatgaaggt ggcatccgcg cgttgcagca
     1801 ccgacaagga ttcctgcacc gccggaccgc cagccccggt gcgggcggtc agcacccaca
     1861 gcactggcac atccggcccc gtgctggcca gcgaccgcag cgccatcaac gtgctgtcat
     1921 cggcccaatg aatgttctcc agcgcaatcg ccagcggggt ctccgccgcc gccgcatgaa
     1981 tcgcgtccgc cagatcgtgc accacccagt agcgcagatc cgccgaaccg cccagccggc
     2041 gcagcgcgtc ggcgtctccg accgggggat cggcgcgtag cgtcgccatg aacagcgcga
     2101 aaaacggcac cgtctgctga tactcgaatg cctcaccgaa cagggtgcgt actccgccct
     2161 tgtcggccag cgccatcacc tcggtcagca gccgactctt cccgatgccc ggcgggccct
     2221 cgatgatcag gatgcccccg tgcccctgca ccagcgccgt caccagcgcg ccgatcacct
     2281 tcagctcgtc tgcccggccc cgaatcggcg gagtggcggg ccgctgaccg ccgacggcaa
     2341 atcggccggt tgaaggccgc acggggtgtc ctgtctaccg tttgctggac tgccgattgc
     2401 agtgcgggcc attctacgca accgtccaac acggtggcat acacgtcgat gctctccagt
     2461 gcgcacacga actgggagtg attactcgcg tccccccgat acccagccca tttccttcaa
     2521 gcgatggcca gtcacggacg agcccgcggc gtccagcgcg tcgagctgct tgggtgtcag
     2581 cgtcaccgag agtgagccca ggttttcctc cacccgggcg gcccgccggg ttcctgggat
     2641 cggcacagag gccacgccga gtgcgtcggc gcggtagcgc agccacgcca gcgccacctg
     2701 tgcggcggtc gcaccctgct gatccgcaac cgacttcacc acctccacca ctgcgaggtt
     2761 ggcatcgagc gcgccctccg cataccgcgg caagttgcga cggaaatccg tcgacgacag
     2821 atcatcggcc gaccggatcg tgcccgtcag gaagccccga cccagaggcg aatacggcac
     2881 gaacccaact ccgagttcgg ccgccgcggg cacgacattg cgttcgacgt cgcggctcca
     2941 gatcgaccac tcgctctcca ccgcggcaat tggatgcacc gcgaccgcgg cccgcagctc
     3001 ctctgccgtc acctccgaca gcccgaggtg gcggaccttg cccgcagcca ccaattcggc
     3061 catggcgcct accgtctcct cgatcggcac agtcacgtcg cgccggtgca tgtaatagag
     3121 gtcgacgacg tcggtctgca gtcgccgcag gctctcgtcg atgctctgat gcacgtatgc
     3181 ggcatcacct cgcgcgacga gtttgcctgc agcacggtcg gccggggtgc caacgatgcc
     3241 gaatttggtt gccagcgtta cttcgtcgcg acggtcggcg agcagcctcg agatcagctg
     3301 ctcgttggcc ccgccaccgt actgattggc ggtgtcgatg aacgtgacac ctagatccac
     3361 ggcgtggttc agcgtcgcca gcgattcggc gtcgtcgacc tcaccataga ccggtgtcaa
     3421 gaccatcgcg ccgaatccga ttgcgctgac tgataattcg tcgcccagct cgaccgcggg
     3481 cacagagttt gcccttgtca tgattcttcc tctcgcttcg gcggtagggg actcgcgacg
     3541 atgcgctaac agtattctca gccgccaggg tgcgttttcc gcagccgctg cccgacaagg
     3601 ctattcatcg cgatcatcgc ttcgtcaaag atgtggatac ccgagtgggt ccaccgcccc
     3661 ttctatcttc gaccgcatgc ccacgggtgt cgatgtcacc gcggacaaca ccacagagcg
     3721 caaaatgctg ccgcgaacac ccaatgcaag atcgctcgcc gtggcgaatc gcgtccacag
     3781 ccgctgcggg tgcgctttcg ccgcgctcaa cgccgaccct gatgtcgtca cggacaacgc
     3841 actgcggcca ctggtgtatc cgacaacatc gacgggctcg gtcactcgac ggacgatcga
     3901 gtcatttaag agatgacgcc ggcaccgttt tcacgacggc caccaattga gtgcagatga
     3961 ggacgcgatg acacgccact tcccgactat gaacacggcc acgacagaca tcaacgtatt
     4021 gatcatcgtc gggcttcgtg cggcatccgt cgatcgcgaa ctcgcgaagg tcgcggccga
     4081 cagtccagct gacggcatag ccttgaatat gttcgacagc ctcaccgaat tgccccggta
     4141 tcgggaacct ttcgagaacc tggagacgcc gagttctgtc ggagacttgc gcagggcagc
     4201 cgcagaggcc gacgcggtgc tcattgtgac gacttaccac gggcgcgtgc ccgccgtcgt
     4261 acacaacgcc atcgattggc tgacgtgacg atgggacgag gcgaatcttc atgacaagcc
     4321 gctcgccgtg atcggccgcg cttcgggctg ctacagcggt gtctggtcac atcaaacgga
     4381 ggacgtcgat cgcattgtgg gacaccgcgt catcgagtcc ctcaccgtgt ccgacctagc
     4441 cgaggcaatc aagaagctcg cctgcgaagt caatgtcggc gactaagaca ggagaaccct
     4501 tgcggtacaa ggcaattgct gcggacccaa ctacgatggc caccgagtcg tttgcgccgg
     4561 tggtcaaggc aacaccgccc tgcgggttcg cgagttcgtg ttgactgtgt cgcggtgaca
     4621 acggcgtcac cgcgcactct cgccgcttca tcggcacgac agcggttccg ataacatccg
     4681 catggctaca tgagccggcg gcgcacctcg aaagccaaac acagctcggc gacatccttt
     4741 gggcgcgaga gagatcggct ggt
//
LOCUS       DASRJA010000802         5998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_902202,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000802 DASRJA010000000
VERSION     DASRJA010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902202"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..753)
                     /locus_tag="VFC01_36170"
     CDS             complement(103..753)
                     /locus_tag="VFC01_36170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN85095.1"
                     /translation="MPESRRRLSPDDRRNELLALGAEVFGERPYDEVRIDEIAERAGV
                     SRALMYHYFPDKRTFFAAVVRAEGDRLFEATSAPPDPGQSLFDQVREGVLAYVRYDEE
                     HPHGAWAAYVGMGSSDPVLRGIEDIDNDRQANRIISRIMDAVGGAMDSKVERDLRVTI
                     YGWLAFTFEMCRQRLLDPSIDAGYVGDACAHALLDAIGRVPGIPSALSDAVAPEHR"
     gene            1013..3616
                     /locus_tag="VFC01_36175"
     CDS             1013..3616
                     /locus_tag="VFC01_36175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85096.1"
                     /translation="MPNALTGDFDLVLEVSESTLERLVASMHQNGFANPDLPSLPHIA
                     RFRIGDDNPVDGEHGSVEAQIGVPRIVLIDGASDRFWLEIGFRARYRADPGSAPLADV
                     IHGTIRALYRVAPIDPHCPGWSKMAGDYLWLRVVRSSVSFEGSAYRTGIPLVAIFDDE
                     GVRARLSQHLAGLLAGQFEPKPHAIGPLFRRMVTRSGPAGAGVAIPLSVDGSAPQGGD
                     LHTITRIFLQGSDFAVAISSDYIVRTVQEQLDPFTGYQKNIHHTRDAGAGGGLILDYH
                     ARVDGSTTEWLGGAVGAPGGIIKITINCHGWASRLYRSGVFNIGSVSINDLDIRASAE
                     QYLMLMFDQSGERLVVSAMGEPAVKLISPLPGLVKAVAENMIRGIASSVMKSKIGEAQ
                     ATLDTMAFTSNKQQFIAQLKTIDADADAFLTGARFDRDGIVVGGAVRVRHRHAPHIAF
                     APATGDHFDAIETWLPGGRVDRYEWSWRWFTNPIEKPPGPAGSRSEPDSFYLERPARP
                     RSRFGPMIGGESPLPGLDGWGEVCLSITGVQVDATTGQFVPVRFGPQCVKFGYQFKMP
                     FEMNGPYLRVCDPLRAVRGGPAPEIGRLKCAAAEEEPVANTLVVFMDSEWHPHVGSQL
                     AGGLRACRRTDAGLLVMILFREGALQTLNADAAAGMSELRAELEAPLLVTDDIDGAWA
                     RWLALNGQTLPEWRLIDPHGITTWVGSDTVDDSKLAEVLNDRLVTSKPALVGGPPHEV
                     VDIGKRFPVELGGRDCPPIPLQRAGTTGSRVLFADTGVASAEAISHLSRDADDPDTPP
                     CVAIVFTGASPQEVRALRAEHDSTAEFVADPDGSLTRKAGVSFTPSAFALDAAGRIVS
                     VQSALPRQSAD"
     gene            3627..5117
                     /locus_tag="VFC01_36180"
     CDS             3627..5117
                     /locus_tag="VFC01_36180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85097.1"
                     /translation="MTLEIAKDPTASYKAKNVFSALVEIARCDPGLTTQEFDFSFEVH
                     YQQGNNWRTVASANRLKAVVDSPMLTTIIGLHSDQIIPRPGSNTRIIEEGGLTAPKFT
                     GDPGADIFVDVDDANDTHRLLPDDTHNFLVWEPLPLLLFHELSHVFHTFIAKDAPQLA
                     DPQLEQFEKEKQVRADDNAFRDALQLPREHPTDYVKVEYGMPTRGGLKFPNCTWSTYG
                     NPWEHCKLCSIATAALGSPINRQIATFRTAKRNFQRLTLGSVPILAPMLDSYRLFGPK
                     VARAVSQDEQLRSATVHYGVQPAVHLIRSVEGYLDAAADTGRAVAAVDAALAGYEAEV
                     AGAVTATHLRAAADAALAGSRALAGQRSAAALDTAAGLFGHVVTSVRASGADPDGPAW
                     VLAGICMFLRAAAQWSTGRPDAAAQLTAAIGAWLATVPLPPDALPTDGLRHELDALRE
                     RVFPESSNRAVFGQRLRAHWRAVDPSLDRLLEQAHFLERSVVCQGT"
     gene            5114..5596
                     /locus_tag="VFC01_36185"
     CDS             5114..5596
                     /locus_tag="VFC01_36185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85098.1"
                     /translation="MTMESVELRETDEITERGARVYLVTVTLMNVSDHPVYAASIITY
                     IRYADADRTLHIGFGTRNDADWPFLSPPRPTPHVEIASGAHIAIEDSIVSPIEYVEET
                     ADGAVQARTIAIDRDVAEIQCTVSFADEPPPMVIDLTSSTPTRDIARHVVRGTWVRPD
                     "
     gene            complement(5622..>5998)
                     /locus_tag="VFC01_36190"
     CDS             complement(5622..>5998)
                     /locus_tag="VFC01_36190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZN85099.1"
                     /translation="PERHADKDVWLTRKGAVDAHQGVMGVIPGSMGTRSYIVRGKGNP
                     ASLCSAPHGAGRRFSRTEARRRFTAADLAARMEGIEYRHGDAWVDEIPDAYKPIDVVM
                     TDSKDLVEVVTTLRQVVNVKGT"
BASE COUNT         1067 a         1935 c         1952 g         1044 t
ORIGIN      
        1 tagccatcgt gcacgcagcc accgacaaat tgtgtcgccg agggtgcggt tgttgtacag
       61 aaaacgcgag taggggcgta aacaagcgtg cgctcggcgc gttcagcgat gctcgggggc
      121 gacggcgtca ctcagcgcgg atgggatgcc cggcacccgg ccgatcgcgt cgagcagggc
      181 atgcgcgcag gcgtcgccga cgtatccggc gtcgatcgac gggtcgagca ggcgctgcct
      241 gcacatctcg aaggtgaacg ccagccagcc gtagatggtg acccgcaggt cgcgttcgac
      301 cttggaatcc atcgcgccgc cgaccgcatc catgatgcgg ctgatgatcc ggttggcctg
      361 ccggtcgttg tcgatgtcct cgatcccgcg cagcaccggg tcggagctgc ccatgccgac
      421 ataggcggcc caggcgccgt gtggatgttc ctcgtcgtat cgcacgtacg ccagcacgcc
      481 ctcacgcacc tggtcgaaca ggctctgtcc tgggtcgggc ggggcgctgg tggcctcgaa
      541 gagccgatcg ccctcggcgc gcaccaccgc cgcgaagaac gtccgcttgt ccgggaagta
      601 gtggtacatc agtgcccgcg acaccccggc gcgctcggcg atctcgtcga tccggacctc
      661 gtcgtacggg cgctcgccga acacctcggc acccaacgcc agcagttcgt tgcgacgatc
      721 gtcgggcgac agccgccgtc gggactcggg catgccgcca ttctagttaa cacatgtaca
      781 acagtgctga aggcttggtg tctgtcgttg acgcgttagc gcgtggcgcg gtgggcggga
      841 cgacattgtc ccgaccgtgc atggatcgca tcgatgatga gatcgacggc ccgagcgcgc
      901 cgccatcgcg cagaagagcc cgatcaaaag ccgactctat tgaccggtgc gtgtgtgacg
      961 atggtcgcgg cgttcggctg cagttgcatg acggggaggg gagggcacgg gcatgcccaa
     1021 cgcattgacc ggcgatttcg atctcgtgct cgaagtcagc gagtccaccc ttgagcggct
     1081 tgtcgcgagc atgcaccaga acggtttcgc caatcctgac ctaccgagcc tgccccatat
     1141 cgcgcggttc cggatcggcg atgacaatcc ggtcgacggc gaacacggct cggtcgaagc
     1201 gcaaatcggg gtgccgcgca tcgtcttgat tgacggggca tccgaccgtt tctggcttga
     1261 gataggcttc cgcgcgcgct accgggcgga ccccggatcg gcaccgctgg ccgacgtcat
     1321 ccatggcacg atccgggcgt tgtaccgggt cgcccccatc gacccccact gtccggggtg
     1381 gtccaagatg gccggcgact acctctggtt acgggtggtg cggtcgtcgg tgtcgtttga
     1441 gggctccgcc tatcgaacag gcataccgct ggtcgccatc ttcgatgacg agggtgtgcg
     1501 ggcgcgcctc agtcagcacc tcgccggatt actcgctggc cagttcgaac cgaaaccaca
     1561 cgctatcggg ccgcttttcc gtcggatggt gacgcggtcg gggccagcag gtgccggcgt
     1621 cgcgattccg ctgtcggtcg acggttcggc gccgcagggc ggggatttgc acaccattac
     1681 gcggattttc ttgcaaggca gcgactttgc ggtcgcgatc agcagcgact acatcgtgcg
     1741 aacggtacag gagcaactcg atcccttcac cggataccag aagaatatcc accacacccg
     1801 agatgccggt gccggcggcg gacttatcct cgactaccac gcccgcgtcg atggcagcac
     1861 caccgagtgg cttggcggcg cggtcggcgc cccaggcgga atcatcaaga tcaccatcaa
     1921 ctgccacggc tgggcctcca ggctgtaccg cagcggcgtg ttcaacatcg gaagtgtgag
     1981 catcaacgac ctcgacatcc gcgcatccgc cgagcagtac ctgatgctca tgttcgacca
     2041 gagcggcgaa cgactcgttg tctcggcgat gggcgagccg gccgtaaagc tcatcagccc
     2101 cctcccggga ctggtcaagg cagtggccga aaacatgatt cgcggcatcg cgtcgtcggt
     2161 tatgaagtcg aagatcggtg aagcgcaagc gacgctggat acgatggcgt tcacctcgaa
     2221 caagcagcaa ttcatcgctc agctcaagac gatcgacgcg gatgccgacg catttctcac
     2281 cggcgcccgc ttcgaccgcg acgggatcgt cgtcggcggt gccgtgagag tacgccatcg
     2341 gcacgcgccg catattgcgt tcgctccagc gaccggagac cacttcgacg cgattgagac
     2401 gtggcttccc ggtggccggg tggaccggta cgaatggtcg tggcgctggt tcaccaatcc
     2461 gatcgaaaag ccacccggcc ctgcaggttc ccgctctgag ccggacagtt tctacctgga
     2521 gcgcccggca aggccacgca gtaggttcgg cccgatgatc ggaggcgaaa gcccactgcc
     2581 cggtctcgac gggtggggcg aggtgtgcct gagcatcacc ggtgtgcagg tcgacgccac
     2641 gacgggccaa ttcgtgccgg ttcggttcgg accgcaatgc gtgaaattcg gctaccagtt
     2701 caagatgccg ttcgagatga acgggccgta cctacgggtc tgtgatccct tgcgcgctgt
     2761 gcgcggcggg ccagcacctg agatcggccg actgaaatgt gccgcggcag aagaggagcc
     2821 ggtcgccaac acgctggtgg tcttcatgga cagcgagtgg caccctcacg tcggatcaca
     2881 gttggctggc gggctgcggg catgccgacg gaccgacgcc gggctgttgg tgatgatcct
     2941 gtttcgcgag ggagcgcttc agaccctcaa cgccgacgct gccgcaggca tgagcgagct
     3001 gcgggcagaa ctggaggcgc cgctgttggt gacagacgac atcgacggcg cctgggcgcg
     3061 gtggctagcg ctaaatggcc agacgctacc cgagtggcgg ctgatcgacc cgcacggcat
     3121 cacaacttgg gtgggcagcg acactgtcga cgactcgaaa cttgccgaag tcctcaacga
     3181 ccggctggtt acgagcaagc ccgccctcgt cggcgggccc ccacacgagg tcgtcgacat
     3241 cggtaagcga tttcccgtcg aactgggggg ccgagattgc ccgccgatac cgcttcagcg
     3301 cgccggcaca accggctcgc gggtgctttt cgcggacacg ggtgtggcgt cagctgaagc
     3361 gatttcgcac ctgagccgag atgccgacga tccggacacg cccccgtgcg tcgcgatcgt
     3421 tttcaccggt gccagcccgc aggaggtgcg tgcgttacgt gctgaacacg acagcaccgc
     3481 cgagtttgtc gccgaccccg acggctcctt gacgcgcaaa gcaggcgtgt cgttcacccc
     3541 gtcggcgttc gccctggatg ccgccggccg catcgtcagc gttcagagtg ccctaccgcg
     3601 ccaatccgca gactgaaggg gagcacatga cactcgagat cgcaaaggat ccgacagcat
     3661 cgtacaaagc gaagaacgtt ttcagtgcgc tggtggagat tgcgcgctgt gatcctggcc
     3721 tgacgacgca ggagttcgac ttcagcttcg aggtccacta tcagcagggg aacaactggc
     3781 ggacggtggc ttctgccaac cggttgaagg ccgtagtcga cagccccatg ctgaccacga
     3841 tcatcggact gcactccgac cagatcattc cccgtccggg atccaatacg aggattatcg
     3901 aagaaggggg gttgactgct cccaagttca ccggcgatcc aggggccgac atcttcgttg
     3961 acgtagatga tgccaacgac acccaccgcc ttcttccgga cgacacccac aacttcttgg
     4021 tatgggaacc gttgccgctg ctgttgtttc acgagctctc gcatgtgttt cacacgttca
     4081 tcgccaagga cgcgccgcag ctggccgatc cacagctaga acagttcgag aaggagaaac
     4141 aggtcagggc cgacgacaac gcgttccgcg atgccctgca gctgcccagg gaacatccga
     4201 cggactatgt caaggtcgag tacggtatgc cgacgcgtgg cgggctgaag tttccgaact
     4261 gcacttggag cacctatggc aacccgtggg agcactgcaa gctctgcagc atcgcaactg
     4321 ccgccctcgg ctcgccaatc aaccggcaga ttgcgacatt ccgaacggcg aagcgcaatt
     4381 tccaacggtt gacgttgggg tcggtgccga tactggcgcc aatgctcgac tcctaccgac
     4441 tattcggtcc caaggtggcg cgcgcggtat cgcaggacga gcaattacgc agcgccacag
     4501 tgcattacgg cgttcagccc gcggtgcatc tgattcggtc ggtcgagggc tatctggacg
     4561 cggcagccga caccggccgc gcggtcgctg cagttgatgc ggccttggct ggctacgaag
     4621 cggaggtggc cggcgccgtg accgcgacac atctccgcgc cgccgcggac gcggcgctcg
     4681 cgggcagtcg cgccctcgcc ggccagaggt ccgcggcagc gctcgacacc gcggccggtc
     4741 ttttcggcca cgtggtgacg tcggtgcgcg cgagcggtgc cgatccggat ggccccgcgt
     4801 gggtgctagc cggtatctgt atgttcctgc gcgccgcagc acagtggtct accgggcggc
     4861 ccgacgccgc agcgcaattg acagcggcga tcggtgcatg gctggcaacc gtgccgcttc
     4921 cccctgacgc cctcccgacg gacggtctac ggcacgagct cgatgcgctg cgcgagaggg
     4981 tctttcccga aagctccaac cgggcagtgt tcgggcagcg gcttcgagcg cactggcgtg
     5041 ctgtcgatcc atctctggat cgcctactgg agcaagcgca cttcctcgag cggtccgtag
     5101 tatgtcaggg cacgtgacga tggagtcggt cgaactgcgc gagaccgacg aaataaccga
     5161 acgtggcgct agggtttacc tcgtcacagt gaccctgatg aacgtgtccg accaccccgt
     5221 ctacgcagcg tcgatcatca catacatccg gtatgccgac gccgaccgga cgctgcacat
     5281 cggtttcgga acgcgcaacg acgccgactg gccgttcctg tctccaccgc gacccacgcc
     5341 ccacgtcgag attgcgtccg gcgcgcacat cgcgatcgag gacagcatcg tgtccccgat
     5401 cgaatacgtc gaggaaacgg ccgacggcgc ggtgcaagcg cggacgatcg caatcgatcg
     5461 tgacgtggca gaaatccaat gcaccgtgag tttcgccgac gagcctccgc cgatggtgat
     5521 cgacttgacg agttcgacgc ctactcgcga catcgctcgg cacgtcgtcc gggggacatg
     5581 ggtacggccc gactgaacgg tccggcgccg agcattaacc gctacgtgcc tttgacgttg
     5641 acgacctgac gcaaggtggt gaccacctcg acgaggtcct tcgagtcagt catgacgacg
     5701 tcgatcggct tgtaggcgtc ggggatctcg tccacccacg cgtcgccgtg ccggtattcg
     5761 atgccttcca tccgcgcggc gaggtcagcc gccgtgaaac ggcgccgggc ttcggttcgg
     5821 gagaaccgcc ggcccgcacc gtggggtgcg ctgcacaggc tggccgggtt gcccttgccg
     5881 cgcacgatgt acgaccgggt gcccatcgaa cccgggatga cgcccatgac gccttggtgg
     5941 gcgtcgaccg cgcccttgcg ggtgagccag acgtccttgt cggcgtgccg ttctggcg
//
LOCUS       DASRJA010000803        10291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2550497,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000803 DASRJA010000000
VERSION     DASRJA010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 10291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10291
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550497"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VFC01_36195"
     CDS             complement(<1..1026)
                     /locus_tag="VFC01_36195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN85100.1"
                     /translation="MSTTQEQVDGAVDGRPDAMRAILERQRQAFAADGAPSVAVRRDR
                     IDRLLALVLDNADEFVAAMSADYGTRPKTGSLFTEILGMVSVIEHTRSHVPQWMRATK
                     LMRAGRLFGLKAEVQPSPLGVVGIIGPWNFPLNLVVLPASAAFAAGNRVMIKMSEITP
                     RTADLLKATAPKYFDPTELDVLTGGPDVAAAFSALPFDHLFFTGSPSVGALVQEAAAR
                     NLVPVTLELGGKNPVVVSPDADINRSAARIAQAWMINGGQVCICPDYVFVPDDRLDKF
                     VDIARDTLRAMFPSIVTNDDYCSSVNDANFDRVVGLIDDAKANGATVETIEPAGESLP
                     DRATRKIA"
     gene            1139..1924
                     /locus_tag="VFC01_36200"
     CDS             1139..1924
                     /locus_tag="VFC01_36200"
                     /EC_number="1.1.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl-alcohol dehydrogenase"
                     /protein_id="HZN85101.1"
                     /translation="MSGERRKVVVIGAGSGIGAAVATHFHDRGDHVLAVDVREHQTPA
                     AEYLQCDLRDAASINTLLGQIGSGWDMLAHVAGIPGTAPAADVLKVNYLGMRLVTEGM
                     LPLLREGGSVVAVASTAALGWDQRIDTLTELLELTDAEAVAKWQAGQDPNYPVYTTSK
                     QAVILYSKRLAGPAWTKYGVRINTVSPGPVETPILSDFEKTMGKDVLDMCRATVGRHA
                     SVDDIVPVISFLGSSEARWITGQDIQVDGGFISCMTAGAPVQL"
     gene            1997..3205
                     /locus_tag="VFC01_36205"
     CDS             1997..3205
                     /locus_tag="VFC01_36205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZN85102.1"
                     /translation="MESPVQDVTADATFDLLRDPYPLFAQKRRESGVFKGSVMDWSKT
                     PESMLPEHLYAAVSFDAVNRVFRDGKVFNSHIYDSTIGLFMGPTILAMEGKKHWEHRN
                     LVSAAFKSKSLLRWEPEIVRPVVNALIDEFIDRGEADLVKDFTFEFPTRVISKLLGLP
                     SEDLPWFRKRAVELISYTVKYKRAFEASAALKDYFLQQIEQRRSKPTEDIIGDLVAAE
                     IDGEKLSDEAIYSFLRLLLPAGLETTYRSSGNLLYLLLTHPDQFRAVQSDHELIPAAI
                     EEGLRFETPLTTVQRYATEDTELEGVELPAGAVIDVCIGSANRDEHRWERSEEFDIFR
                     KRLPHISFAAGEHTCMGLHLARMETRVAVECLLTRLTDIKLVTDDNPHIWGQPFRSPT
                     AIPVTFDAAG"
     gene            3209..3844
                     /locus_tag="VFC01_36210"
     CDS             3209..3844
                     /locus_tag="VFC01_36210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZN85103.1"
                     /translation="MAQRRQRRERGSISVEEILNGAFDVAAEVSIDNLSMPLLARQLD
                     VGVTSIYWYFRKKDELLDAMTDRALAQYEFTVPSIDASNWRESLRNHAQTMRRQFLED
                     PVLCDLVLIRGHYGMHALHGALHEIERPVAALVEAGLTPEQAVETYGAISVHTRGSVV
                     LQRLQQKTEGFPAQRNDHRYIGFADDIDFDYILDSILDHAEYLISLSTASP"
     gene            complement(3819..4667)
                     /locus_tag="VFC01_36215"
     CDS             complement(3819..4667)
                     /locus_tag="VFC01_36215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HZN85104.1"
                     /translation="MGKLDNKVAVVSGAARGQGRSHAVNLAAEGASIIALDICADLEG
                     NTYPLSRPEDLDETARLAEKEGVRVYTAIVDVRERSAVWKAIADGVDEVGRLDIVVAN
                     AGICAMGKGQTIQSWSDTIDTDLVGVFNVIQASLPHVNDGASIIATGSLAAQLGSATN
                     QGPGGSAYSLAKQIVARYVNDLSVQLAKRMIRVNAVHPTNVNTDMLHNEGMYKVFRPD
                     LKEPTREDAEASFPAMQAMPIPYIEPQDVSNTIVFLASDDSRYITGTQLRVDAGGFVK
                     AKPWKG"
     gene            complement(4667..6136)
                     /locus_tag="VFC01_36220"
     CDS             complement(4667..6136)
                     /locus_tag="VFC01_36220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN85105.1"
                     /translation="MLIDGELVTAASGEEFDNVSPATGLVLGTTSAAEATDMDRAIAA
                     ARRAFDESDWSTNRALRKRCLEQLQAGIEADKENLREELIAEVGCPAMTTQSAQLDWP
                     LAEALRYPGKLIDDFEWERTLDGGGLFGERNVRAVVKEPVGVVAAVTPSNFPIEVILN
                     KLGPALATGNTVVLKPDPNTPWNATRLGRLIAEHTDIPAGVVNVVPTPSNEVAGILGT
                     DPRIDMISFTGSTNVGKLLMRQGADTMKRTFLELGGKSVSIVLDDANPAAILGAAVGV
                     CVHAGQACAATTRMLVHRSLYDDAVATITAAYGAVPVGDPVQPETLVGPVISAAQKER
                     VLSAIERARRDGAQITVGGGEVNNLPAHLRDGHYVAPTVIVGVDNSAAIAQEEVFGPV
                     LIVLPFDDDDEAVRIANDSAYGLAGAVMSRSIERGMNIARRIRTGSFGVNGGMFYGAD
                     APFGGYKNSGVGRQCGVEGFEQYLETKTIGWRKPRQKEG"
     gene            6309..7523
                     /locus_tag="VFC01_36225"
     CDS             6309..7523
                     /locus_tag="VFC01_36225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HZN85106.1"
                     /translation="MEGVRVLEVAQFTFVPAAGAILADWGADVIKVEHPVRGDTQRGF
                     INMGGFQLDPNRHPLMEHPNRGKRSVGIDVSTPGGQEVLYEIAKTADVFLTNYMPAQR
                     QKNKFDVEHIRAVNPNIIYARGSAYGDKGPERDTGGFDGTAFWTRSGVGHALTPEELG
                     GALSQGIPAFGDSIGGMNIAGGISAALFHRERTGEAVELDVSLLSTAWWAGGASVTQG
                     METGETMRSAMPDATGPSVNPFMANYLTSDGGTINLCIVSPTGYIRDAFEHLGLPELA
                     DDPRFSDVMPLIENAAAAVELIREKIRSKPFDYWRQHLKTMKGQWAPFQSFIDLANDE
                     QAIANDMIVEVEAGDGGKPFKVVRGPVQFNHEPLETTRAPQASEHTEIVLMELGMEWD
                     RIEELKDKGAIA"
     gene            complement(7592..9766)
                     /locus_tag="VFC01_36230"
     CDS             complement(7592..9766)
                     /locus_tag="VFC01_36230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZN85107.1"
                     /translation="MIEHKPTFCRICEPLCGMIATVEDGRLVALRPDKGHPLSSGFAC
                     QKGIAFAEVHNDPDRVTAPLRRGPRGFEPVSWDEAMTDIAERLTAIYRRHGSGAVGWY
                     MGNPAAFSYSHLLWLQAFVKGLGGDTHYFTASSQDTNSRLIASQLLYGTPTSVPIPDL
                     TRTDFLVLMGANPVVSHGSFLTAPRIKDRMHDIVKRGGRVLVIDPRRTETAAKFEWLG
                     VVPDADAYLLLSLLHVLFAENLAARDRANRQADGVEWLQAVAEPFTPEATVAHTGIEA
                     DVVRSLARDLAKTPRAAIYGRFGTSVGRNATLTTYLIDAVNLVAGNLDRPGGSVFSGL
                     EMPGQKWGTKVMGAALRRSYEKRRSRIGGFRAAIGSEPAALMAKEITTPGNRQIKAMF
                     VSAGNPVLSVPNGNELEGALDGLELSVALDFYVTETTAHCDYVLPVTTMYERDDFPVT
                     FQTFQATPFRQATEAVAAPAGQARTEWEIIDDLMQRMKVRTPVFTVMSGARKVLGLFG
                     VRLTPRLMIDGMIRLAAGGNRFGLRRGGLTFKRLTERHPHGVVLAPHLRTDVLDDVVA
                     YRRGRIRLAHADIANEVAALVRREEPEGFPLRMIGMREPRSENSWMHNSPLLMRGGRG
                     HHALMHVDDAAELQICDGDEVQVSSPFGRITVPVTSTKDLVAGVIAIPHGWGHKGTGG
                     WQLANRAGGSNVNQLMSSAPEHVESLSGMSWLTGVPVRVDRI"
     gene            9846..10220
                     /locus_tag="VFC01_36235"
     CDS             9846..10220
                     /locus_tag="VFC01_36235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011855362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZN85108.1"
                     /translation="MRWIVDGMNVIGSRPDGWWRDRGRAMTALVDRLEQWAAAEGADV
                     TVVFERPPTPPIESSVITVTHAPEPAANSADDEIVRLVGADADPAEIRVATSDRALSD
                     RVRAAGASVYPAERLRNLIEPR"
BASE COUNT         1801 a         3284 c         3351 g         1855 t
ORIGIN      
        1 cgcgatcttg cgagtggcgc ggtcgggcag cgattcaccc gcaggttcga tcgtctcgac
       61 cgtggcgccg ttggccttgg cgtcgtcgat caggccgacg acgcggtcga agttggcgtc
      121 gttgaccgag gagcagtagt cgtcgttggt gacgatcgac gggaacatcg cgcgcagcgt
      181 gtcgcgggcg atgtcgacga atttgtcgag tcggtcatcg ggcacgaata cgtagtcggg
      241 acagatgcat acctggccgc cgttgatcat ccacgcctgc gcgatgcggg ccgccgatcg
      301 attgatatcg gcgtcgggcg acaccaccac cgggttcttg ccgcccagtt ccagcgtcac
      361 cggaacgagg ttgcgtgcgg ccgcctcctg cacgagcgcg cccaccgacg gcgaaccggt
      421 gaagaacaga tggtcgaatg gcagcgccga gaaagccgcc gcgacgtcgg gaccgccggt
      481 gaggacgtcg agttcggtcg gatcgaagta cttcggcgcc gtcgccttca acaggtccgc
      541 tgtgcgcggg gtgatctccg acatcttgat catcacccgg ttgcccgccg caaacgcggc
      601 cgaggcaggc agcaccacga gattgagcgg gaaattccac gggccgatga tcccgacaac
      661 acccagcggt gacggctgga cctctgcctt cagaccgaac agccgaccgg cgcgcatcag
      721 cttggtcgcc cgcatccact gcggcacatg cgatctggtg tgctcgatca ccgagaccat
      781 gcccaggatc tcggtgaaca acgatcccgt cttcggccgg gtaccgtagt cggccgacat
      841 cgcggcgacg aattcatcgg cgttgtccag gacgagtgcg agcagccggt cgatgcggtc
      901 acgcctcaca gcaacgctcg gtgcgccgtc ggccgcgaac gcttgccgct gacgctcgag
      961 gatcgctctc atcgcgtcag gccggccgtc gactgcgccg tcaacctgct cctgcgttgt
     1021 gctcattcgt ccgtcccgta ctcattgtct ggcggaccag aattgtttac tgtcgtcctt
     1081 actgtcggcc attcgaatag tacgccgaca tcgcggtgcg gcggaaaggg ttggtcgaat
     1141 gagcggtgag cgacgcaagg tcgtggtgat cggtgcggga tccggcatcg gagcggcggt
     1201 cgcgacgcac ttccacgacc gcggcgacca cgtgctggcg gtggacgtgc gcgagcacca
     1261 gactccggcg gccgagtatc tacagtgcga tctgcgcgac gcagccagca tcaacacgct
     1321 gttgggccag atcggttcgg ggtgggacat gctcgctcac gtcgctggta tccccgggac
     1381 ggcgcccgct gccgacgtgc tgaaagtgaa ctacctcggc atgcgactgg tcaccgaagg
     1441 catgctgccg ctgctgcgtg agggtggatc ggtggtcgcc gtcgcgtcga cggccgcgct
     1501 tggctgggac caacgcatcg acacgcttac cgaactgttg gagctcaccg atgccgaggc
     1561 ggtcgcgaag tggcaggcgg gccaggatcc gaactatccg gtctacacca cgtccaagca
     1621 ggcggtgatc ctctacagca agcggcttgc cggccccgcc tggaccaagt acggggtgcg
     1681 gatcaacacc gtgagcccgg ggccggtcga gacgccgatt ctctccgatt tcgagaagac
     1741 catgggcaag gacgtgctcg acatgtgccg cgctaccgtc ggccggcacg caagcgtcga
     1801 tgacatcgtg cccgtgattt cgttcctggg gtcgtcggag gcccgctgga tcaccggcca
     1861 ggacatccag gtcgacggcg gcttcatcag ctgtatgaca gcaggggcgc ccgtacagct
     1921 gtaggttcgc tatactgtaa gctttacagt acctatagca ggatgcctgt ccattcgacg
     1981 atgctggaga tcagcggtgg aaagcccggt gcaggacgtc acggccgacg cgacgttcga
     2041 cctccttcgt gatccgtacc cgctcttcgc gcagaagcgg cgggagtctg gcgtcttcaa
     2101 gggcagcgtc atggattggt ccaagacacc ggaatcgatg ctgcccgagc atctgtacgc
     2161 cgcggtctcc ttcgacgccg tcaatcgcgt cttccgcgac ggaaaggtgt tcaactcgca
     2221 catctacgac agcaccatcg gtctgttcat gggcccgacc atcctcgcga tggagggcaa
     2281 gaaacattgg gaacaccgca atctcgtgtc tgcggcgttc aagtcgaagt ccttgctgcg
     2341 gtgggagccg gagatcgtcc gtccggtcgt caacgcgctg atcgatgagt tcatcgaccg
     2401 cggtgaggcc gatctggtca aggacttcac gttcgagttt ccgacgcgcg tgatctcgaa
     2461 gctgctgggc ctgccctccg aggacctccc ctggttccgt aaacgtgccg tcgagctgat
     2521 cagttacacg gtcaagtaca agcgcgcgtt cgaggcgtcc gcagcgctga aagactactt
     2581 cctgcaacag atcgagcagc ggcggtccaa gccgaccgaa gacatcatcg gcgatctggt
     2641 cgctgcggag atcgacggcg agaagctgtc cgacgaagcg atctactcgt tcctgcggct
     2701 cttgcttccg gccggcctgg agaccaccta ccgctcgtcg gggaacctcc tctacctatt
     2761 gctcacacac cccgaccagt ttcgagccgt gcagtccgat cacgagctga ttccggcggc
     2821 cattgaagag ggcttgcgat tcgagacgcc gctgaccact gttcagcggt acgcgacgga
     2881 ggacacggaa ctggaaggcg tggagcttcc ggccggagct gtgatcgacg tgtgcatcgg
     2941 gtcggccaac cgagacgaac accgctggga gcgctcggag gaattcgaca tcttccggaa
     3001 acgcctgccg cacatctcgt ttgccgccgg tgagcacacc tgcatgggtc ttcacctcgc
     3061 ccggatggag acgcgggtcg cggtggaatg cctgctgact cggctgaccg acatcaagct
     3121 cgtcaccgac gacaatccac acatctgggg tcagccgttc cggtcgccga cggcaattcc
     3181 ggtgacgttc gacgctgcgg ggtgaccgat ggcccaacgg cgccagcgac gcgagcgcgg
     3241 gtcgatctcc gtcgaggaga tcctcaacgg cgctttcgac gtggcggcgg aggtctcgat
     3301 cgacaacttg agcatgccgc ttttggcacg gcagctggat gtcggagtga cgagcatcta
     3361 ttggtacttc cgcaagaagg acgagcttct cgacgcaatg accgaccggg cactggcgca
     3421 gtacgaattc accgtgcctt caatcgatgc gtccaattgg cgtgagtcgc tgcgcaatca
     3481 cgcgcagacg atgcgtcggc agttcctgga ggaccccgtt ttatgcgacc ttgttctcat
     3541 tcgtgggcac tacggcatgc acgcgcttca cggtgccctg catgagatcg aacgacccgt
     3601 cgccgcgttg gtcgaggcag ggctgacgcc cgagcaggcc gtcgaaacct acggcgcgat
     3661 ttctgtgcac actcgcggat cggtcgtgct gcagcgtcta cagcagaaga ccgagggatt
     3721 tcccgctcag cgcaatgacc accggtacat cgggttcgcc gacgatatcg acttcgacta
     3781 catcctcgac agcatcctgg atcatgccga gtatctgatc agcctttcca cggcttcgcc
     3841 ttgacgaacc cgcccgcgtc cacccgcaac tgcgtgccgg tgatgtagcg ggagtcgtcg
     3901 ctggcgagga acacgatggt gttcgaaacg tcttgcggct caatgtaggg aatcggcatg
     3961 gcctgcatgg cggggaagga tgcttcggca tcctcacgcg tcggctcctt cagatcgggc
     4021 cggaacacct tgtacatccc ctcgttgtgg agcatgtcgg tgttcacgtt cgtcgggtgc
     4081 acggcgttga ctcgaatcat ccgcttggcc agctggacgg agaggtcgtt gacgtagcgg
     4141 gcaacaatct gcttggcgag gctgtacgcc gaaccgccgg ggccctggtt ggtcgcacta
     4201 ccgagttggg cggccagcga tcccgtcgcg atgatcgagg caccgtcgtt gacatgcggc
     4261 aggctggcct gaatcacgtt gaaaacaccg accagatcgg tgtcgatggt gtcggaccac
     4321 gattggatgg tctggccctt gcccattgcg cagatgcccg cgttggcgac gacgatgtca
     4381 agtcgaccca cctcgtccac gccgtcggcg atcgccttcc acacggcgct gcgctcgcgc
     4441 acgtcgacga tcgcggtgta cacacgcaca ccttccttct ccgccagccg cgccgtctca
     4501 tcgagatcct cgggccgcga cagcggatag gtattgccct ccaggtcggc acagatgtcc
     4561 agcgcgatga tgctggcgcc ctcggcagcg agattgactg cgtgggaacg gccttggccg
     4621 cgcgcggcgc ccgatacgac tgcgaccttg ttgtccagct ttcccattag ccttctttct
     4681 gtcggggctt tcgccagccg atggtcttgg tctcgagata ctgctcgaag ccctcgacgc
     4741 cgcactgccg gccgacaccg ctgttcttgt agccgccgaa tggcgcgtcg gcgccgtaga
     4801 acatgccgcc gttgacgccg aacgagccgg tgcggatgcg gcgcgcgatg ttcatgccgc
     4861 gctcgatgga ccgcgacatg actgcgccgg caagcccgta cgcgctgtcg ttggcgatgc
     4921 ggaccgcctc gtcgtcgtca tcgaacggca gcacgatgag caccgggccg aagacctctt
     4981 cctgtgcgat cgccgcactg ttgtcgacgc cgacgatcac ggtaggcgcc acataatggc
     5041 cgtcgcgcag gtgcgcgggc aggttgttga cctcgccgcc gccaacggtg atctgcgcgc
     5101 cgtcgcggcg ggcccgctcg atcgcgctca gtacgcgctc cttctgtgcg gcgctgatca
     5161 ccggtccgac aagggtttcc ggctgcacgg gatcgccgac cggcaccgcc ccgtatgccg
     5221 cggtgatggt ggcgaccgcg tcgtcgtaca gcgaccggtg gacgagcatg cgggtggtcg
     5281 ccgcgcatgc ctgtccggcg tgaacgcaga ctccgaccgc ggcgcccaga atcgcggcgg
     5341 ggttcgcatc gtcgagcacg atcgagacgg actttccgcc gagttcgagg aacgtccgct
     5401 tcatggtgtc ggcgccttgc cgcatcagca gcttgcctac gttcgtggag ccggtgaacg
     5461 agatcatgtc gatgcgagga tcggtcccta gtattccggc cacctcgttg gacggggtcg
     5521 gcacgacatt cacaacgccg gccgggatgt cggtgtgttc agcgatgaga cggccgaggc
     5581 gggtggcgtt ccatggggtg ttggggtcgg gtttgagcac gacggtgttg ccggtcgcca
     5641 gtgcgggtcc gagcttgttg aggatgacct cgatcgggaa gttggagggg gtgacggcag
     5701 cgacgacgcc caccggctcc ttgacgacgg cgcgcacgtt ccgctcaccg aacaagccgc
     5761 cgccgtccag tgtgcgttcc cattcgaagt cgtcgatcag ctttccggga tagcgcaggg
     5821 cctccgccaa cggccaatcc agttgcgcgg actgggtcgt catcgcggga cagccgactt
     5881 cggcgatgag ttcctcgcgc agattctcct tgtcggcctc gatccccgct tgcagctgtt
     5941 ccaggcagcg cttgcgtagc gcgcggttgg tcgaccaatc ggattcgtcg aaggcacgcc
     6001 gggcggcggc gatggcgcgg tccatgtctg tcgcctcggc ggcgctagtg gtgcccagca
     6061 ccaggccggt ggcagggctg acgttgtcga actcttcgcc actggcggcc gtcacgagct
     6121 cgccgtcgat cagcatccgg gcttcggcgt gcttggctgc ccgcctgccc agctcgacac
     6181 tggtctcggt ccgttcttcg acgtcgctca cctgcacctc gtacaatcgg acactgcggg
     6241 acgatcaact gtaaacatta cagtaggata tttcaacagc gcaggagagg gctcacttga
     6301 tcaaggtgat ggagggcgtt cgcgtcctcg aagttgctca attcacgttc gtgcctgcgg
     6361 cgggggcgat cctcgccgac tggggcgcag acgtcatcaa ggtggagcac ccggtgcgcg
     6421 gcgacaccca gcgcggcttc atcaacatgg gtggcttcca actggacccg aaccggcacc
     6481 cgttgatgga acatccgaat cgcgggaaac gaagcgtcgg catcgacgtg tcgacgccgg
     6541 gcgggcagga agtcctgtac gagatcgcca agaccgccga cgtgttcttg acgaactaca
     6601 tgcccgcgca gcggcagaag aacaagttcg acgtcgagca catccgcgcc gtgaacccga
     6661 acatcatcta tgcgcgtggc agcgcctacg gcgacaaggg gccggaacgg gacaccggcg
     6721 gtttcgatgg cactgcgttc tggacgcgca gcggcgtggg ccatgcgctg acgcctgagg
     6781 aactgggtgg cgcactgtcg caaggcatcc cggcgttcgg cgactccatc ggcggaatga
     6841 acatcgccgg cggcatctcc gcggcgctgt tccatcgtga gcgcacgggc gaggcggtgg
     6901 agctcgacgt gtcattgctg agcacggcct ggtgggccgg cggcgcaagc gttacccagg
     6961 ggatggagac gggtgagacg atgcgttcag cgatgccgga tgcaacaggc ccgagcgtaa
     7021 acccgttcat ggctaactac ctgacctccg acggcggcac catcaacttg tgcatcgtca
     7081 gcccgaccgg gtacatccgc gatgcgttcg aacatctcgg actgcctgag ttggccgacg
     7141 atccgcgctt ctccgacgtg atgccactca tcgagaatgc cgcggccgcc gtcgaactca
     7201 tcagagagaa gatccgcagc aagcccttcg actactggcg tcagcacctc aagacgatga
     7261 aaggccagtg ggcgccgttc cagagtttca tcgacctcgc caacgacgaa caagcgatcg
     7321 ccaacgacat gatcgtcgaa gtcgaggcag gcgacggcgg caagccgttc aaggtcgttc
     7381 gcggacccgt ccagttcaac cacgagccgc tcgagaccac gcgcgccccg caggcctccg
     7441 aacacaccga gatcgtgctg atggaactcg ggatggagtg ggaccgcatc gaggaactca
     7501 aggacaaagg cgccatcgcc tagggtctcg ccgaaactgt attccagcag agaaagttcg
     7561 agtgcaggcc tgccggaata ccgtttcgcg gtcaaatgcg gtcgacgcgt accgggacgc
     7621 cggtcagcca ggacattccg gacagtgatt cgacgtgttc gggtgcgctc gacatgagct
     7681 ggttgacgtt ggatcccccg gcccggttgg cgagctgcca acccccagtg cccttgtgcc
     7741 cccagccatg tggaatcgcg atcacgccgg cgacgagatc cttcgtcgac gtgacgggca
     7801 cggtgatacg gccgaagggc gagctcacct gcacctcgtc gccatcgcag atctggagtt
     7861 ccgcggcgtc atcgacatgc atcagggcgt gatgcccgcg tccgccgcgc atcaacaacg
     7921 gcgagttgtg catccacgaa ttctccgatc gtggctcgcg catgccgatc atccgcagcg
     7981 ggaacccttc cggttcctca cgtcggacca acgcggctac ctcgtttgca atatcggcgt
     8041 gcgccagccg aatccggccc cgccgatagg caaccacgtc atccaataca tcggtgcgca
     8101 aatgcggggc caacacgacg ccgtgcgggt ggcgttccgt caaccgcttg aacgtcaggc
     8161 cgccgcgacg tagcccgaac cggtttccgc ccgccgcaag gcggatcatg ccgtcgatca
     8221 tcagtcgcgg cgtgaggcgg acaccgaaca gccccagcac ttttcgagca ccggacatga
     8281 ccgtgaacac cggcgtccgg accttcattc gttgcatcag gtcgtcgatg atctcccact
     8341 cggtgcgcgc ttggcccgcc ggcgccgcga cggcttcggt ggcctgacga aatggggtgg
     8401 cctggaacgt ctggaacgtc acgggaaagt cgtcgcgttc gtacatcgtt gtcaccggca
     8461 ggacatagtc acagtgcgca gtggtctcgg tgacgtagaa gtcgagtgcg accgagagct
     8521 ccaagccgtc gagcgcccct tccagttcat tgccgtttgg caccgacagc acgggattgc
     8581 ccgcgctgac gaacatcgcc ttgatctgcc gattgcccgg cgtcgtgatc tccttggcca
     8641 tcaacgccgc cggctcggag ccgatagccg ctcggaatcc gccgatgcgg gagcgccgct
     8701 tctcgtagga ccggcgcagc gcggcaccca tgactttggt tccccacttc tggcccggca
     8761 tttccagccc gctgaacacc gacccgccgg ggcggtcgag attgcccgcg acgaggttga
     8821 ccgcgtcgat cagataggtg gtcaaggtgg cgttacgacc gacgctggtc ccaaaccgtc
     8881 cgtagatcgc agctcgcggc gtcttggcca ggtcgcgggc cagtgaccga acgacatccg
     8941 cttcgatgcc ggtgtgcgcg acagtggcct cgggggtgaa cggttcggcc actgcttgta
     9001 gccactcgac accgtcggct tgccggttgg cgcggtcgcg ggccgccaga ttctcggcga
     9061 acaacacgtg caacagcgac aagagcaggt aggcgtccgc gtcggggacg acgccgagcc
     9121 attcgaactt ggcggcggtc tcggtcctgc gcgggtcgat caccaatacg cggccgccgc
     9181 gcttgacgat gtcgtgcatc cggtccttga tgcgcggcgc cgtcagaaaa ctcccgtggg
     9241 agacaacagg gttggcgccc atcaggacga ggaagtccgt tcgcgtcaga tcgggtatgg
     9301 gcaccgatgt cggcgtaccg tacagcaact ggctcgcgat gagccggctg ttcgtgtctt
     9361 gcgaggacgc ggtgaaataa tgagtgtcgc cgccgagacc tttgacgaac gcctgcagcc
     9421 acagcagatg cgagtagctg aacgccgccg ggttgcccat gtaccagccg accgcgcccg
     9481 aaccgtgcct gcggtagatg gcggtcagcc gttcggcgat gtccgtcatc gcctcgtccc
     9541 agctgaccgg ttcgaagcca cgcggcccgc ggcgcagcgg cgcagtgacg cggtcggggt
     9601 cgttgtgaac ctcggcgaac gcgatgccct tttggcaggc gaaccccgac gacagcggat
     9661 ggcccttgtc cgggcgtagc gcgacgagcc gcccgtcctc gacggtggcg atcatgccgc
     9721 acagcggttc gcagatccgg cagaaggtcg gcttgtgctc gatcacccca cgaccatact
     9781 gtaattctta cagttacatc gccacttcga gcagtgcatg cttggcgtgg gcatagcctg
     9841 ggcgggtgcg gtggatcgtc gacggcatga acgtgatcgg ctcccggccc gacgggtggt
     9901 ggcgcgaccg cggccgtgcg atgaccgcgt tggttgaccg actcgagcaa tgggccgcgg
     9961 ccgaaggtgc tgacgtcaca gtggtgttcg agcggccgcc gaccccacct attgaatcgt
    10021 cggtgatcac tgtgacgcat gcgccggagc cggccgcgaa ttcggccgac gatgagattg
    10081 tccggctggt gggtgccgac gccgatcccg cggagatccg cgtggccacc tcggaccgag
    10141 cgctgtccga ccgagtccgg gcagccggtg cgtcggtcta cccggctgaa cgcctgcgca
    10201 acctcatcga accgcgctag ccgcgagtca caggggatac tgtaagattt acagttgctt
    10261 ctacttgggt gcccacgacg gaggatcgcg c
//
LOCUS       DASRJA010000804         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_2267699,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000804 DASRJA010000000
VERSION     DASRJA010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267699"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFC01_36240"
     CDS             <1..242
                     /locus_tag="VFC01_36240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar epimerase"
                     /protein_id="HZN85109.1"
                     /translation="KALGRPIRFEELPPEAARRAMLAQGFPEGFVTGYLLLQAEAVDR
                     PALVTDTVEEILGRPATDFATWAADHAESFRGMAA"
     gene            239..904
                     /locus_tag="VFC01_36245"
     CDS             239..904
                     /locus_tag="VFC01_36245"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZN85110.1"
                     /translation="MKLTLLGATGGVGREIFSQAVATGHLVTAVVRNPSKLPADVDVV
                     RQDLATPDLEVLAAAMRDVDAVISAVGPHRAAEAGIVAPATAAITHAMDCARVRRLVV
                     ISAAPVGVVPSTHQPHPPRYDPGDDQLMRSVLSPLIRRLFGPLYADLAAMEDQLRDGS
                     LDWTSIRPPRLTNGPLTRTYRTAIDRNVRHGLFISRRDVAHLMLTVLQQPETVRHAVG
                     VAY"
     gene            977..1825
                     /locus_tag="VFC01_36250"
     CDS             977..1825
                     /locus_tag="VFC01_36250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZN85111.1"
                     /translation="MTAIRAAITQTTWTGDKESMLDKHEQFAREAKAQGAQVICFQEL
                     FYSPYLGITEDVKYYDFAEPADGPIVQRFAAIAAELSMVAVLPIFEEEQPGIYYNTAV
                     VVDADGRVLGKYRKNHIPNLDKFWEKFYFRPGNLGYPVFQTAVGPIGVYICYDRHFPE
                     GWRELGLNGAQIVFNPNATKPGLSNRLWEIEQPAAAAANGYFVAACNRVGREDNEYGE
                     LAVTFYGSSQFCDPQGNVVGAMGSDQHEEVVVRDLDMDMIRQIRNTWQFYRDRRPDSY
                     AAITKP"
     gene            1839..>2305
                     /locus_tag="VFC01_36255"
     CDS             1839..>2305
                     /locus_tag="VFC01_36255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016888920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZN85112.1"
                     /translation="MTTTLIKNGTVINATGSAAADVLVDGETIVAVLNPGSGLFGLDL
                     EGNVDSVVDAAGKYVIPGGIDPHTHMELPFGGTQASDTFETGTLAAAWGGTTSIIDFA
                     VQRYGERVEEGLATWHAKAAGNACIDYGFHQIIGGVDEFSLKAMDGLIDEGVT"
BASE COUNT          439 a          761 c          720 g          385 t
ORIGIN      
        1 tgaaggctct cgggcggccg atcagattcg aagagcttcc accggaggcg gcccgccggg
       61 cgatgctggc ccaaggtttc cccgaaggct tcgtcaccgg gtacctgctg ctgcaagccg
      121 aggccgtaga ccggccggct ctggtcaccg acacggtcga ggagatcttg ggccggccag
      181 cgaccgattt cgctacctgg gcggctgacc acgctgaatc attcaggggg atggccgcgt
      241 gaaactcacc ctgctcggcg cgaccggtgg cgtcggccgc gaaatcttta gccaggccgt
      301 cgctaccggt cacctggtca cggctgtggt gcggaacccg tcgaagctgc cggcggacgt
      361 cgacgtcgta cggcaggatc tggcaactcc ggacctcgaa gtcctcgccg ccgcaatgcg
      421 cgacgtcgat gcagtgattt ccgcggtggg cccgcaccgt gcagccgagg ccgggatcgt
      481 tgcaccagcc actgccgcca tcacacacgc catggactgt gcacgcgtcc gccgcctcgt
      541 ggtgatcagt gccgcgccgg tgggcgttgt gccttcgacc caccaaccgc atccgcctcg
      601 ctatgacccc ggcgacgacc agctgatgcg ctccgtcctc agccctctca tccgacgact
      661 cttcggccct ctctatgccg acctcgccgc aatggaggac cagttgcgcg acgggagcct
      721 ggactggacc tccatccggc caccgcggct caccaacgga ccgcttaccc gcacgtaccg
      781 gactgcgata gaccgcaacg tacgccacgg cctgttcatc tcgcggcgag atgtcgcgca
      841 cctgatgctg accgtgctcc aacaacctga gaccgtacgc cacgccgtcg gcgtcgccta
      901 ctaagggcgc tagggccgct gccgtctgaa accttggaca agtcagaatg agtgacatcc
      961 aaggggaggc agaagcgtga cagcgatccg agcggcgatc acccagacga cctggaccgg
     1021 ggacaaggaa tcgatgctcg acaaacacga gcagttcgcc cgggaggcca aggctcaggg
     1081 tgcgcaggtg atctgcttcc aggagctgtt ctactcgcca tatctgggca tcaccgagga
     1141 cgtcaagtat tacgacttcg ctgaacccgc cgacgggccc atcgtgcagc gcttcgccgc
     1201 cattgccgct gagctgtcga tggtcgcggt gctcccgatt ttcgaagagg agcagccggg
     1261 catctactac aacaccgcgg tggtggtcga tgccgacgga agggtgctcg ggaagtatcg
     1321 caagaaccac attccgaacc tcgacaagtt ctgggagaag ttctacttcc gccccggcaa
     1381 cctcggatac ccggtattcc agaccgccgt cggcccgatc ggggtctaca tctgctacga
     1441 ccgccacttt ccggaaggct ggcgcgagct cggcctgaac ggtgcccaga tcgtctttaa
     1501 cccgaacgcc acgaaacccg ggctttccaa ccggctctgg gaaatcgagc agccggcggc
     1561 cgccgcggcc aacggctact tcgtcgccgc gtgcaaccgg gtcggccgtg aggacaacga
     1621 gtacggcgag ctagccgtca ccttctacgg ctccagccag ttctgcgatc cgcagggcaa
     1681 cgtggtcggt gcgatgggga gcgatcagca cgaggaggtc gtggtgcgcg acctcgacat
     1741 ggacatgatc agacagatac gcaacacctg gcagttctac cgggaccgac ggccggactc
     1801 gtacgccgcg atcaccaagc catgatcgga agaatcctat gaccacaacc ttgatcaaaa
     1861 acggaaccgt gatcaatgcc acgggttctg ctgccgccga cgtgctcgtt gacggcgaga
     1921 ccatcgtcgc ggtactgaat cctggatcag gattgttcgg acttgatctc gaagggaacg
     1981 ttgactccgt cgtcgacgcc gcgggtaagt acgtcatccc gggcggcatc gacccacata
     2041 cccacatgga gctgccgttc ggcggcaccc aggcctctga cacgttcgag accggtactt
     2101 tagccgccgc ctggggcggc acgacgtcga tcatcgactt tgcggtgcag cgctacggcg
     2161 agcgcgtgga ggaagggcta gcgacctggc acgccaaagc ggcggggaac gcctgcatcg
     2221 actacggctt ccaccagatc atcggtggcg tcgacgagtt ctcgctcaag gcgatggacg
     2281 gattgatcga cgagggcgtg acgag
//
LOCUS       DASRJA010000805        19594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_378877,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000805 DASRJA010000000
VERSION     DASRJA010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 19594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19594
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378877"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /gene="mycP"
                     /locus_tag="VFC01_36260"
     CDS             complement(<1..1223)
                     /gene="mycP"
                     /locus_tag="VFC01_36260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion-associated serine protease
                     mycosin"
                     /protein_id="HZN85113.1"
                     /translation="MGVLLAALILALLSAPPAAAIEPPTIDPAVVPPDETGPDQPTEQ
                     RRVCSAPTALPNSNFADKPWPNDYLRISDAQKFATGAGVTVAVIDTGVNGSPRVPAEP
                     GGDFVDKAGNGMSDCDSHGTLTASIIAGRPAPTDGFIGVAPDARILSLRQTSEAFQPV
                     GSRTDPNDPNTTQTAGSLRSLARAVVHAANLGAQVINISEAACYKVTRPINETGLGAA
                     INYAVNVKGAVIVVAAGNTGQDCSQNPSPDPGTPADSRGWRRVQTIVSPAWYSPLVLT
                     VGGIAQNGQPSNFSMNGPWVDAAAPAENIVALGYDGAPVNALQGQDGPIPITGTSFAA
                     AYVSGLAALLKQRFPDLTPAQIMNRITATARHPGGGVNNEVGAGVIDPVAALTWDVPA
                     GPEQVPYKVKQLPPPV"
     gene            complement(1430..1687)
                     /locus_tag="VFC01_36265"
     CDS             complement(1430..1687)
                     /locus_tag="VFC01_36265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HZN85114.1"
                     /translation="MPTKTVIVGSAIGLHARPAAIIAEAVVNAGVPVTLSMDDGEPVD
                     AGSALMIMTLGAGNGAEVTVASDDDEALRTVAELVQQDLDA"
     gene            complement(1713..3743)
                     /locus_tag="VFC01_36270"
     CDS             complement(1713..3743)
                     /locus_tag="VFC01_36270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HZN85115.1"
                     /translation="MTSTAPQPIINTDLVLLDVDAGGDKQAVIGRLVSRLADAGRTHD
                     TEGLIAAAMAREEQSATGLPGGIAIPHCRSPHVDTASIGFARLSPAVDFGAPDGPADL
                     AFLIAAPEAGGADHMKLLSSLARALVRKDFVESLREATSPDEVVELVDGVVNATVEKP
                     KPVAEPVKAKTLVAVTACPTGIAHTYMAADSLVAAAKKAGATLHVETQGSSGSTPLSP
                     ATIAEADAVIFATDVGVKDKHRFAGKPVVASGVKRAINEPDKMVAEALAAAADPAAAR
                     VQGDAGAPTTAAAPAGGVGWGTRTRQILLTGVSYMIPFVAAGGLLIALGFLFGGYDIA
                     NTPAGEPKPLAFGMNALGNHIAITNTLTNLPPGGLTQYLGAVLFTLGQLAFMFLVPAL
                     AGYIAFAIADRPGIAPGFTAGAVAVFVGGGFIGGIVGGLIAGFTALWISRIAAPRWLR
                     GLMPVVIIPLVASLAVGLLMFMLLGRPLAAITSGLTNWLSGLSGTSVIILGVILGLMM
                     CFDLGGPVNKAAYAFATAGLNVTDPASLRIMAAVMAAGMVPPLAMALASTIRPGLFTE
                     PERENGRAAWLLGASFISEGAIPFAAADPLRVIPSMMFGGAITGALIMAFDVTLKAPH
                     GGIFVFFAIGNLLWFVISLVVGTVAGALAVIAAKEFIKPNVEAEEAPALAAA"
     gene            complement(3740..4735)
                     /locus_tag="VFC01_36275"
     CDS             complement(3740..4735)
                     /locus_tag="VFC01_36275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HZN85116.1"
                     /translation="MIVTVTPNPSIDRTVTLAGPLTRGAVHRVSSVTSEPGGKGVNVA
                     RTLTLAGLDAMAVLPAPAADPLVAALEASAVPFRCVPTAGAVRTNLAITEHDGTTTKL
                     NEPGATLDAVTLDALTRSVTASAESASWVVLSGSLPPGVPDHWYADVIALLEPYPCRV
                     AIDTSEGPLAALVASFDRAAPDLIKPNAEELAGVLGYSPQALEAAVAQGDPEPVVSAA
                     LRLVDRGVRAVLATLGPAGAVLVDETGSWMASPPPIVARSTVGAGDASLAGYLRADVG
                     GAVPPQRLQMAVAYGSAAAALPGSALPTPAQIDLDAVLVTPIAPISPTTNTKVSP"
     gene            complement(4732..5499)
                     /locus_tag="VFC01_36280"
     CDS             complement(4732..5499)
                     /locus_tag="VFC01_36280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HZN85117.1"
                     /translation="MYAEERQQAIASLVIGQGRASVAELAQAYDVTTETVRRDLAVLD
                     KAGVLRRVHGGAVPARALHLVEPGVGERETTRSEHKDAIAAAATEFFPLGGATVLLDA
                     GTTTARIAAQLPTDRELVVITNSVPIAARLAAMPSISLQLLGGRVRGLTQAAVGEQTL
                     RVLDTLRVDIAFIGTNAISVRHGLSTPDSDEAAVKRAMVNAANFVVVAADSSKIGRED
                     FVSFAPISSVDTLITDSEINAADRDQLTESGVEVVVA"
     gene            5542..7302
                     /gene="ptsP"
                     /locus_tag="VFC01_36285"
     CDS             5542..7302
                     /gene="ptsP"
                     /locus_tag="VFC01_36285"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HZN85118.1"
                     /translation="MFGFTTVRVDLSRISCNLLHMTASSPPTSLPAAGTVLHGVPVVP
                     GVVYAPVIRPGRLPGVDLTNEADIEETDRPAEAARFAAAATAVATRLRERATHATGAA
                     SEVLAATAALAQDRAWLGAAEKRIKDGTPAVAAVVAAAEQFVDMFTQLGGLMAERVTD
                     LRDIRDRVIAQLSGLPEPGVPLPDIPSILCAEDLAPADTAGLDPNLVVALATTLGGPT
                     SHTAIIARQLGIPCVVAVDGLDDVAAATMVLVDGTLGTVTVSPDHAAAGEAVAAAERE
                     AEQAAHWSGPGATADGHAVSILANVQDGAAARAAGETPAEGVGLFRTELCFLNRETEP
                     SVEEQADIYADVLEAFSGRKVVIRTLDAGSDKPLRFVGHPDEANPALGVRGIRIAEDN
                     PGLLDRQLQAIATAAQRTASQPWVMAPMIATAQEAESFAAQVRSHGLTPGVMIEVPAA
                     ALLADRILEHVDFLSIGTNDLAQYTMAADRMSADLATLTDPWQPAVLTLVAMAVRAGA
                     AAGKPVGVCGEAAADPLLACVLTGLGVTSLSAAAAAIQGVGAKLARVTLQQCRDAADA
                     VLGTATAADARAAALSALGN"
     gene            7330..8307
                     /locus_tag="VFC01_36290"
     CDS             7330..8307
                     /locus_tag="VFC01_36290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZN85119.1"
                     /translation="MPAITADTLTLPRIAAPSPTDTERPVRSITTGPHGYEGEGFPVV
                     RAFAGVSAADLDPFVHMDQMGEVEYQPGEPRGTDWHPHRGFETVTYMIDGRFAHQDSH
                     GGGGLITDGATQWMTAGSGILHIETPPAELVESGGVFHGIQLWVNLPRTDKFVAPKYQ
                     AIEGNQVRLLSSDDGGALVRVIAGEVDGNQGPGATYTPITLAHATIEPGARLNMPWNR
                     DFNALVYVLSGRGAVGPVSHPIHQGQLAVLGPGDRITVAAEPTQESRRPALEVLLLGG
                     KPLREPVFQYGPFVMNSKSELIEAMEDFQAGKFGTVPPDALMPHRPRRG"
     gene            9544..9978
                     /locus_tag="VFC01_36295"
     CDS             9544..9978
                     /locus_tag="VFC01_36295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85120.1"
                     /translation="MRSPHPIPTTLELLDRLVSAGLTMSHVPAAGETVVLRSASNLSA
                     GGDSIDLTEESHPGFIDIAIRAVRAIPGIGYAGVDLITPDITARPTATNYIVGEVEFS
                     PAMLAHFPLAGTSRDIAGAVPQYYLDHGAGGTGKAQSATSPS"
     gene            complement(10188..10367)
                     /locus_tag="VFC01_36300"
     CDS             complement(10188..10367)
                     /locus_tag="VFC01_36300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85121.1"
                     /translation="MHRAAGHADARGAGAREQLHALCDSGRRVLVAVSKQEFRAVADA
                     ARHGNEHHVAMILVT"
     gene            10366..10854
                     /locus_tag="VFC01_36305"
     CDS             10366..10854
                     /locus_tag="VFC01_36305"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HZN85122.1"
                     /translation="MPLRFGAITDILARRECDVELLHLIEETGREVGARLPPGSKVGV
                     LGTTGTIRSDVYGRVMRAAGFEVLYLDDSRQSEVHAAIYNEVDGLKSGTIVSARVRDL
                     VTQAARELIDRGARCVVLGCTELPEALPVESLGGVPVIDPSVVMASRLILRTADAPGT
                     AS"
     gene            complement(10851..12053)
                     /locus_tag="VFC01_36310"
     CDS             complement(10851..12053)
                     /locus_tag="VFC01_36310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZN85123.1"
                     /translation="MSEVLVLGSGFAGLWAALGAARRLDELGSANGDVHITVLSSQPY
                     HDIRVRNYEADLTPCRIPLGDVLDPAGVAHITADVTAIDAISRTVKSSVGTHVYDRLV
                     VALGSRVVKPDIPGLREFGFDVDTYGGAMALQRHLEALAGRPHTPAASTVVVVGAGLT
                     GIETACELPARLTALFPDAGGRVVLVDHNPHVGSDMGASARPVIEAGLNSTGVQTRLG
                     VGVTVIEADRVTLSTGETLAASTVVWCAGMRANALTADLGVALDRLGRLPVDEYLRVN
                     GVPDVFAAGDVAAAMMDDTHVSVMSCQHGRPMGRFAGYNVISDLLGEPMLSLRIPWYV
                     TVLDLGPAGAVYTEGWDRQVVATGDEAKATKRTINAQRIYPPLNGNREAILAAAAPTL
                     QSRPSAAG"
     gene            complement(12050..14200)
                     /locus_tag="VFC01_36315"
     CDS             complement(12050..14200)
                     /locus_tag="VFC01_36315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HZN85124.1"
                     /translation="MSSAAGTAAVLRHVGYRWRARDPEFDALRRASRAAIVLPIAAAV
                     SFTVGGGSQTPLFTIFGSVALLILVDFPGNRPARALAYSGLGINGAILITLGTLVAPH
                     PWVSVAVMFVLGVAVIFAGVLSEIVAAGQRATLLMFVLPACIPVGPLPERLLGWLIAL
                     ALCVPAALFLFPPRHHDELRANAARVCNRLAGRLEGTVSARDVTKAMNALYVTFLGAD
                     YRPVALTAGSRALVRVVDDLGWLSDRITDETGRMLGPMKDPAVRVLRDSAAVLRLPSV
                     AGRAERSADLRDALTELRSVSQGRYREDIAELLGESSDGAAVEIGRTLLNRRTIAATI
                     AVTGRIIRNAAAADARPVWARVLGRRLPDTGAADWVMPETVAVAAITKGFLATRAVVL
                     RNSIRTGLGLAVAVAVTHVFPVEHGFWVVLGAMSVLRSSALTTGTRVLRAVAGTAVGF
                     VLGAIVIELLGVDPVVLWILLPIVAFGSAYVPEVASFVAGQAAFTMMVLINFNLIVPT
                     GWQVGLIRVEDVVVGALVGIVVSVLLWPRGATAAVSKAIDDARAVGAEFLTQAVLRVT
                     RGASEDATDRVIALSRAGLEASRTVDDAVRQYLSENGGMTELRAPVVRAANRAIRLRA
                     AAELIADVVPPPLGVYERTREVLESHADAIEARLTGDPGRELHTISDDFVLSLRADAT
                     NDDLAISAALPLTTVAANLGELELLYPRAVASAG"
     gene            complement(14193..14759)
                     /locus_tag="VFC01_36320"
     CDS             complement(14193..14759)
                     /locus_tag="VFC01_36320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZN85125.1"
                     /translation="MASVASREAYFETGLDVLSDLGYGGLKLAEVCNRLGVTTGSFYH
                     YFSGWPAYTKELVAHWMQGRTVQVIEAVRGEPDPRRRIDTLINVGLALPHGAEAAIRV
                     WSSVDPQVYPVQAAVDQQRFDIMYESAFEILQNKRQAQLFAAWGVYLLVGYEQATLPR
                     DSDALEWIAGQLLDALDSGRFASVPDGE"
     gene            14927..15565
                     /locus_tag="VFC01_36325"
     CDS             14927..15565
                     /locus_tag="VFC01_36325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZN85126.1"
                     /translation="MPQHTASRGPGRPPAAKAAETRERIVQAAREVFSELGYDAATFQ
                     AIAIRADLTRPAINHYFASKRVLWREVVEQTNALIVSAGMERAQNETNLLARLSAFFS
                     VAMQADSEDRSAAAFLVTSVLESQRHPELASDDHDSLTASREFMSWAVNDAIKLGELT
                     TDTDVNHLVEMLVAVMWGMGFYAGFVGSHDELNSVVHKFELLMANKLWNLHE"
     gene            15635..16585
                     /locus_tag="VFC01_36330"
     CDS             15635..16585
                     /locus_tag="VFC01_36330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZN85127.1"
                     /translation="MTDVSEVRLVRTDGDSWDITESVGATALGVAAARAAETARPDAL
                     IRDEFAYLLTASAGPAWAQMASADDEWLGDNEDARRMQAMARDYQAVRTHYFDGYFTA
                     AVRTGIRQVVILAAGLDSRAYRLDWPAGTTVFEIDQPKVLEYKTSTLDAHGAVPTSRR
                     VPVAVDLRDDWPAALVSAGFDPAQPTAWLAEGLLPYLPGDAQDRLFEMITANSAAGSQ
                     IAVEAFNIQPSQYSPEKRAARRERSAQLRERLGLDVDVDTLMYTDDARADAAEWLAAH
                     GWRVAAVPSAEEMTRLGRPTTENLVEEALDSVFVHARLEG"
     gene            16671..18080
                     /locus_tag="VFC01_36335"
     CDS             16671..18080
                     /locus_tag="VFC01_36335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011854189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZN85128.1"
                     /translation="MTTESVAPEAVKAPATDIPAIVGKLRDTFKSGRTRSVEWRKQQL
                     RALEAMMTENEAAIAAALEQDLGRSPFEAWLADIASTAGEAKDAAKNVRKWMRRKYRL
                     LEMSQLPGRGWIEYEPYGTVLVIGAWNFPFVLTLGPAVGAIAAGNTMVLKPSEVCPAS
                     SALMAELVPKYLDNDAIVVIEGDGAVSQELIAQGFDHICFTGGTEIGRKVYEGAAPHL
                     TPVTLELGGKSPVIVSADADIDVAAKRIAWTKFINSGQICIAPDYVLADAKIRDQLVD
                     KIKAAVTTFEAENPGGRRIVNQRHFDRLTASLAATKGDVVIGGGSDSSKISIQPTVVV
                     DPDPAEPLMTDEIFGPILPVVTVQSLDDAIGFVNSRPKPLSAYLFTKTKSIRDRVIKE
                     VSAGGMVINHLLFQFSTNKLPFGGVGPSGMGAYHGKFGFEQFSHKKTVMTKPTRPDVG
                     AFIYPPYTEKAFKLARRLF"
     gene            18093..18956
                     /locus_tag="VFC01_36340"
     CDS             18093..18956
                     /locus_tag="VFC01_36340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZN85129.1"
                     /translation="MPGVQDRVIVVTGAGGGLGREYALTLAKEGASVVVNDLGGARDG
                     TGEGHSMADQVVKEIKDAGGRAVANYDSVAEPEGAANIIKTAVDEFGKVDGVVSNAGI
                     LRDGTFHKMEFANWDAVLKVHLYGGYNVIRAAWPHFREQSYGRVVVATSTSGLFGNFG
                     QANYSAAKLGLVGLINTLAQEGAKYNIKTNAVAPIAATRMTEDILPKEVLEKLTPEYV
                     APVVAYLCTEEVPETASIFIVGGGKVQRAALFQTDGVTFPDVPSVDDVAGKWGEITDL
                     SAAQPATFSLR"
     gene            18964..>19594
                     /locus_tag="VFC01_36345"
     CDS             18964..>19594
                     /locus_tag="VFC01_36345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZN85130.1"
                     /translation="MKALVAQELSGPSGLVYTDVEDVGGEDAIVVDVGAAGVSFPDLL
                     LLRGEYQLRLEPPFIPGMEVAGVVRSAPYESAFKPGQRVTALAMLGGWAEQVVVPAEN
                     IRPTPDDLDDAEAVALLGNYQTMYFALAKRGALRHGETVLVLGSAGGVGTAAVQIAKA
                     LGAKVIAMVHRPHATEFVESLGADVVLPLTEGWLQAVKDATSGRGVDLVV"
BASE COUNT         3372 a         6473 c         6638 g         3111 t
ORIGIN      
        1 accggcggcg gaagttgctt gactttgtac ggcacctgct ccgggccggc gggaacatcc
       61 cacgtcaacg cggcgaccgg gtcgatgact ccggcgccca cctcgttgtt gacgccgccg
      121 ccgggatgcc gcgccgtcgc ggtgatccga ttcatgatct gcgctggggt gaggtcggga
      181 aagcgctgct tgagcagcgc cgcgagcccg gaaacgtagg cggccgcgaa cgacgtgccg
      241 gtgatgggga tcggcccgtc ctggccctgc agcgcgttga ccggagcacc gtcgtagccg
      301 agcgcgacga tgttttccgc tggggcggcg gcgtccaccc aggggccgtt catcgagaag
      361 ttgcttggct gcccgttctg ggcgatcccg ccaacggtca gcaccagggg cgagtaccac
      421 gccgggctga cgatcgtctg gacccgcctc catccgcgcg aatcggcggg tgtgcctgga
      481 tcgggcgacg ggttctgcga acagtcctgg ccggtattgc ccgccgcgac gacgatgacc
      541 gcgcccttga cgttgacggc gtagttgatc gccgctccga gcccggtttc gttgatcggc
      601 ctggtcacct tgtagcaggc ggcctcgctg atgttgatca cttgcgcccc gaggttggcg
      661 gcgtgcacga cggcacgggc caggctgcgc agtgagcccg cagtctgggt ggtgttcggg
      721 tcgttcggat cggtgcgcga accgaccggt tggaacgctt ccgatgtctg acgcagcgac
      781 aggatccgcg cgtccggtgc gacgccgatg aacccgtcgg tgggcgcggg gcgaccggcg
      841 atgatcgatg cggtcaatgt gccgtggctg tcgcagtcgc tcatcccgtt accggctttg
      901 tcgacgaaat cgccacccgg ctcggcgggc actcgcgggg agccattcac cccggtgtcg
      961 ataaccgcga cagttacccc ggcgccggtt gcgaacttct gcgcgtcgct gatcctcagg
     1021 tagtcgttcg gccacggctt gtccgcgaag ttcgaattcg gcaacgcggt gggcgccgag
     1081 cacacccggc gctgctccgt tggctggtcg gggcctgtct cgtccggcgg caccacggcc
     1141 ggatcgatgg tcggaggttc gatcgctgcg gcggggggag cactcaataa cgccagaatc
     1201 agcgctgcta gcagcacacc gatacggcgc acgcgcgatc acccctttcg acgtcggacc
     1261 ggccagcagt gtgctggcga gcccacagca aaccgtgttc cgaccgggcg gcggcgcaaa
     1321 ttctcacgtc aagccgattg gcccaatgca cgcagccaat cttaagggga tgccggaggg
     1381 ggtagatccg ctaactggcc ttagccgggt cgcggggttc gggcccgaac taggcgtcga
     1441 ggtcttgctg gaccagctcc gcgacggtgc gcagcgcttc gtcgtcgtcg gaggcgacgg
     1501 tcacctcggc accgttgcct gcgcccaacg tcatgatcat cagcgccgag ccggcgtcga
     1561 ccggctcacc gtcgtccatc gagagcgtga cgggcacgcc ggcgttgacg acggcttcgg
     1621 cgatgatggc cgccggcctg gcgtgcagtc cgatggccga gccaacgatg acggttttgg
     1681 tgggcattgg ttctccttac tgatggatgg gtctaggcgg cggcgagggc cggggcttct
     1741 tcggcctcga cgttgggctt gatgaactct ttggcggcga tgacggccaa cgcgcctgcc
     1801 accgtcccga ccacaaggga gatgacgaac cacagcagat tgccgatggc aaagaacacg
     1861 aagatgccgc cgtgcggtgc cttcagcgtg acgtcgaacg ccatgatcaa tgcaccggtg
     1921 atcgcgccgc cgaacatcat cgacgggatg acccgcagcg ggtcggccgc cgcgaacggg
     1981 atggcgccct cggagatgaa cgatgcgccg agaagccacg cggcccggcc attttcccgc
     2041 tcgggctcgg tgaacagtcc tggccggatc gtggaggcca gcgccatcgc cagcggcggc
     2101 accatgccgg cggccatcac cgcggccatg atccgcagcg atgcgggatc ggtgacgttc
     2161 aggccggcgg tggcgaacgc gtaggccgcc ttgttgaccg ggccgccgag gtcgaagcac
     2221 atcatcaggc cgaggatgac gccgaggatg atgaccgacg tgcccgacag gccgctgagc
     2281 cagttggtga ggccggaggt gatggcggcc aacggacggc cgagcaacat gaacatcaga
     2341 aggccgacag ccagggatgc gaccagtggg atgatcacga ccggcatcaa gcctctgagc
     2401 caccgcggtg cggcgattcg gctgatccac agcgcggtga acccggcgat cagaccgccg
     2461 acgatgcctc cgatgaagcc accgccgacg aacacggcca ctgcacccgc ggtgaacccc
     2521 ggcgcaatac ctgggcggtc ggcgatcgcg aaggcgatgt agccggcaag tgcaggtacc
     2581 aggaacatga atgcaagctg tccgagcgtg aacagcaccg cacccagata ctgggtcagg
     2641 ccgccggggg gcaggttggt caacgtgttg gtgatggcga tgtggttgcc gagagcgttc
     2701 attccgaagg ccagcggctt cggctccccc gcaggagtgt tggcgatgtc gtacccgccg
     2761 aacaggaatc cgagcgcgat caacaggccg cccgcggcga cgaacgggat catgtagctc
     2821 acgccggtca gcaggatctg ccgagtgcgg gtgccccagc cgacaccgcc tgccggtgcc
     2881 gccgcggtgg taggcgcgcc cgcgtcaccc tgcacgcgcg cggcggcggg atcggctgcg
     2941 gcggcaagtg cttcggccac catcttgtcg ggttcgttga tggcgcgttt gacaccggag
     3001 gcgacgacag gcttgcccgc gaaccggtgt ttgtccttga cgccgacgtc ggtggcgaag
     3061 atgaccgcat cggcctcggc gatggtcgcc ggcgacagcg gcgtgcttcc cgacgaccct
     3121 tgtgtctcca cgtgcaacgt cgcgcccgcc ttcttggctg cggcgaccag cgagtcggcg
     3181 gccatgtacg tgtgcgcgat gccggttggg catgcggtga ccgcgaccag cgtcttggcc
     3241 ttcaccggtt cggctacggg cttgggcttt tcgaccgtcg cgttgacaac accgtcgacc
     3301 agttcgacca cttcgtcggg gctggtggcc tcgcgcagcg actcgacgaa gtccttgcgc
     3361 accagggcgc gggccaggct ggacagcagc ttcatgtggt cggctccgcc ggcctcaggg
     3421 gcagcgatca ggaacgccag atccgccggt ccgtccggcg cgccgaaatc gaccgccgga
     3481 ctcagccggg cgaacccgat cgatgcggtg tcgacatggg gcgatcggca gtgcgggatc
     3541 gcgatgcccc cgggcagccc ggtggccgat tgttcctcgc gtgccatcgc cgcggcgatc
     3601 aggccctcgg tgtcgtgggt gcggcctgcg tcggcgagtc tgctgacgag ccggccgatg
     3661 acggcctgct tgtctccgcc ggcatcgacg tcgagcaaga cgaggtcggt gttgatgatc
     3721 ggctggggag cggtgcttgt catggcgata ctttcgtgtt ggtggtgggt gagatcgggg
     3781 caatcggtgt caccagaacg gcatcgaggt cgatctgcgc cggcgtgggc agcgcggatc
     3841 caggcagtgc ggccgcggcg ctgccgtagg cgaccgccat ctgcagccgc tggggaggta
     3901 cggcaccgcc cacgtcggca cgcaggtagc cggccagcga ggcgtcgcct gcaccgacgg
     3961 tgctgcgcgc gacgatgggc gggggactgg ccatccagct tccggtttcg tcgaccagca
     4021 cggcgccggc ggggccaagc gtggcgagca ccgcgcgcac gccgcggtca acgagcctga
     4081 gcgcggcgga gaccaccggt tctggatcac cttgggcgac tgcggcttcc agcgcctgcg
     4141 gcgagtagcc gagcacaccg gcgagctcct cggcattcgg tttgatcagg tcgggcgcgg
     4201 cccggtcgaa ggatgcgacg agggccgcga gtggaccttc cgaggtgtcg atcgcaaccc
     4261 ggcacggata cggctcgagc aaggcgatca cgtcggcgta ccagtggtcc ggcactcctg
     4321 gcggcagtga acccgacagc accacccatg aagcggactc ggcgctcgct gtcacggatc
     4381 gcgtcagggc gtcgagggtg acggcgtcga gagtcgcgcc cggttcgttg agtttcgttg
     4441 tggtgccgtc gtgttcggtg atcgcgaggt tggtccgcac ggctccggca gtcggcacgc
     4501 acctgaacgg cacggcgctt gcttcgagcg ctgccaccag cggatcggcg gctggggccg
     4561 ggagcaccgc catggcgtcc agaccggcga gcgtcagcgt gcgcgcgacg ttgacgccct
     4621 taccgccggg ctcgctcgtc accgacgaaa cccggtgtac cgcaccgcgg gtgagtggtc
     4681 cagcgagcgt gaccgtgcgg tcgatgctcg ggttgggcgt gacggtgacg atcatgcgac
     4741 cacgacctcg acgccgctct cggtcagctg atcgcgatcg gcggcgttga tctccgagtc
     4801 ggtgatcagg gtgtcgacgc tggagatcgg ggcaaagctg acgaagtcct cccggccgat
     4861 cttcgacgaa tcagcggcaa ccacaacgaa attcgcggca tttaccatgg cccgtttgac
     4921 cgccgcctcg tcgctgtccg gtgtggaaag gccgtggcgc acgctgatcg cattggtgcc
     4981 gatgaacgcg atgtccaccc gcaatgtgtc cagcactcgc agcgtctgct cgccgaccgc
     5041 ggcctgggtc agaccgcgaa cccggccgcc gaggagttgc agagagatgg acggcatggc
     5101 ggccagccgg gcggcgatcg gcaccgagtt ggtgatcact acgagctcac ggtcggtcgg
     5161 cagctgggcc gcgatacgcg cggtagtggt cccggcgtcc agcaggacgg tggctccgcc
     5221 gagcggaaag aactcggtgg ccgccgcggc gatggcgtcc ttgtgctcgg agcgagtggt
     5281 ctcacgttca ccgacacccg gttcgacgag gtgcagtgcc cgggccggta cggcaccgcc
     5341 gtgcacccgc cgcagcacgc ctgctttgtc caggacggcg aggtcgcggc gcaccgtctc
     5401 ggtggtgacg tcgtaggcct gcgccagctc ggccaccgag gcacggccct gcccgatcac
     5461 cagcgaggcg atcgcttgct gacgctcttc ggcatacatg aggctccgtt tgtgtggtta
     5521 taggcccata ttgatttggt tatgtttggt tttactactg ttcgtgttga cttgtcaaga
     5581 atttcttgta atctgcttca catgaccgcc tcatccccgc ccacgtcact tcccgcagcg
     5641 ggcacggttc tgcacggagt tccggtggtc cccggcgttg tgtacgcgcc cgtgatccga
     5701 ccgggtcggc ttcccggcgt cgacctcacg aacgaggcag atatagagga aaccgatcgc
     5761 ccggccgagg ccgcgcggtt cgcggcggcg gctactgccg tcgcaaccag gttgcgggag
     5821 cgcgcgacgc atgccaccgg cgccgcctcg gaggttctcg ctgccaccgc agcgctggcg
     5881 caggaccgcg catggttggg ggccgcggaa aagcgcatca aggacggcac acccgctgtc
     5941 gctgcggtcg tcgcagcggc cgagcagttc gtcgacatgt tcacccaact cggcggcctg
     6001 atggccgaac gcgtcaccga tctccgtgac atccgcgacc gcgtcatcgc gcaactgagc
     6061 ggcctgccgg aaccgggcgt cccgctgccc gacattccgt cgatcctgtg cgccgaggat
     6121 ctcgcgcccg cagacaccgc ggggctcgat ccgaacctcg ttgtcgccct tgccaccacg
     6181 ttgggcgggc cgaccagcca caccgcgatc atcgccaggc agctcggcat tccgtgtgtc
     6241 gtcgccgtcg acgggctcga tgacgtcgcg gccgcgacga tggtgctcgt cgacgggacg
     6301 ctcggcaccg tgacggtgtc gccggatcac gccgccgcag gcgaggccgt cgcagcggca
     6361 gagcgcgagg ccgaacaggc cgcgcattgg tcaggtcccg gcgccaccgc cgacggccat
     6421 gccgtgtcga tccttgccaa cgtccaagac ggtgccgccg cccgcgcggc cggcgaaacc
     6481 ccagccgagg gcgtcgggct gttccggacc gaactgtgct ttctcaaccg tgaaaccgag
     6541 ccaagcgtcg aggagcaggc cgacatctac gccgacgtgc tcgaggcatt ctcgggtcgc
     6601 aaggtggtga tccggacgct ggacgccggc tcggacaagc cactgagatt cgtcggccac
     6661 cccgatgagg ccaaccccgc gctcggcgtg cgcggcattc gcatcgccga ggacaacccc
     6721 gggctgctgg accggcagct gcaggccatc gcgaccgcag cgcaacgcac cgccagccaa
     6781 ccgtgggtga tggcgccgat gatcgccacc gcccaagagg ccgaaagctt tgccgcgcag
     6841 gtgcgttcgc acggtttgac gccaggtgtg atgatcgagg tgcccgctgc cgcgctctta
     6901 gccgaccgga ttctcgaaca cgtcgacttc ctgtcgatcg gcaccaacga cctggcgcag
     6961 tacacgatgg ccgcagaccg catgtcggcc gatctggcca cgctgaccga tccgtggcaa
     7021 ccggcggtgc tgacgttggt ggcgatggcg gtgcgggccg gcgcggcggc gggcaagccg
     7081 gtcggcgtgt gcggtgaggc cgccgccgac ccgttgttgg catgcgtgct gaccgggctc
     7141 ggcgtgacat cgctgtcagc ggcggccgcc gcaattcagg gcgtcggcgc gaagcttgcc
     7201 cgggtcacgc tgcagcagtg ccgcgacgcc gccgacgcgg tgctgggcac cgcaaccgca
     7261 gccgacgcgc gcgcggccgc gctgtccgcg ctcgggaatt aatcggactg cggtccggtt
     7321 gactgctgca tgcctgccat caccgccgac accctgacgc tgccgcgcat tgccgccccc
     7381 tcgccgaccg acaccgaacg cccggtccga tccatcacca ccgggccaca cggctatgaa
     7441 ggtgagggct tccccgtcgt tcgggcgttc gccggagtca gcgccgccga cctcgacccg
     7501 ttcgtccaca tggaccagat gggtgaggtg gagtatcagc cgggtgagcc gaggggcacc
     7561 gactggcatc cgcaccgcgg gttcgagacc gtcacctaca tgatcgacgg ccgcttcgcg
     7621 catcaggatt cgcacggcgg cggcggactg atcacagacg gcgccacgca gtggatgacg
     7681 gccggatcgg gcatcctgca catcgagacc ccgcccgccg aactcgtcga gagcggcggc
     7741 gtgttccacg gcatccagct gtgggtgaac ctgccgcgca cggacaagtt tgtggcgccg
     7801 aagtaccagg ccatcgaagg caaccaggtc aggctgctgt cgtcggacga cggcggcgcg
     7861 ctggtccgcg tcatcgccgg tgaagtcgac ggcaatcagg gaccgggcgc gacgtacacg
     7921 ccaattaccc tggcgcacgc caccattgag cccggtgcgc ggttgaacat gccctggaac
     7981 cgcgacttca acgcgctcgt ctatgtgctg tccggacgcg gcgctgtcgg acctgtaagc
     8041 catccgatcc accagggaca gctggccgta ctcggaccgg gcgaccggat caccgtggcc
     8101 gccgaaccga cacaagagtc acgcagaccc gcgcttgagg ttctgctcct gggcggcaag
     8161 ccactgcgcg agccggtgtt ccagtacggc ccgttcgtga tgaactcaaa gtccgaactg
     8221 atcgaggcga tggaggactt tcaggcgggc aagttcggca ccgttccgcc ggacgccctc
     8281 atgccgcacc ggcctcggcg aggctagctc atcgacaaag gtgcgtggcg cgtcgtcttg
     8341 ggctgcacgg atctgcctga acccttgccc attgagagct tgagcggtgt gctgatcatc
     8401 catccttcgg tggtcagtgc ccgccgcctg atcatcggag gcgcaggcac cgcggggaca
     8461 tcaacagaag ggcgctgctg atggccccgc cgccttggtg tcgtggcgtt cggtgatgcg
     8521 aacggttgga aaccggttcg cggcgattca ggacctgtgc ccggatgtgg acgccgcagt
     8581 gacgccgacg gttcgccgaa gccagcgaga gcaaattgcc ggcgaatttt ggctgatatg
     8641 gcgagcgccg acgataacgc ggcggcgcct ctcgtcggag tgtcggcgtc gtacaccctg
     8701 tgcatgatgc ggagccgcca cttccaattg ttcacgcgtc gcgcaggcac cggcgggcgg
     8761 ccttgcagcg tgacggagaa cgttagcggg taggcctcgt gccaccatgc gaagggcaag
     8821 aacgtgagat cgcccggtcc agccactccg cggccgagta tgtgcgcgca gcggcacgag
     8881 cggggattgg gtcaaagtcg gcgctcatcg agcacgccgc gatcagcaga ggtctagctt
     8941 cgactcgttg gagcagcacg gaggtcgtga tacaggtgcc gggagacact ggcccaccga
     9001 tcttcttcca cgagatgaat ggcccgcgga gcatgggtgg gaggttttac tgcgacgaaa
     9061 agaccgtcgc gcgagaccta ctcaaacgcg catgtctgaa cgtggccatc tcgcaggcct
     9121 tcgagccgag cgagcttcgc gagggattgc ggtacgcaga gagcatcgga taccccgtgg
     9181 tcgtgaagcc gaccaacctc gcgcgtggca tcggtgttac cgcgaacatt caaagaccgg
     9241 aggaattttc aacagcatgg ctgcgagcgc gtaccgcgaa tcagggatgg cttcacgtgg
     9301 cggcgacgct tgctcggtcc gtccgtggag ctcccgggca cagtcgcgcc cggaattgcc
     9361 ggcctaccgc gaactgatcc gtgacgcgag ctcgacggcg gggcagcgcc gatggcgccc
     9421 agtccttgtc gaaaagcact tccaaggcaa cgacttcagg gccttcgtcg tcggtgacgg
     9481 ccatgcgacc gtacgcgaac tgatcgaccg caagaacgcg atccgagcag agaacgcata
     9541 cctgtgcgat caccccatcc catcccgacg acacttgagt tgctggaccg cttggtcagc
     9601 gccggcctga ccatgtcgca tgtcccagct gcaggcgaga ccgtcgtgct tcgaagtgcg
     9661 tccaacctgt cggccggcgg ggacagcatc gatttgactg aggagtcgca ccccggcttc
     9721 atcgatattg cgatcagagc ggttcgtgcg atcccgggaa tcgggtacgc gggcgtcgac
     9781 ctgatcacac cggacatcac ggcccgaccc acggccacca attacatcgt cggcgaggtc
     9841 gagttctcac ccgccatgct cgcgcacttt ccgctggccg gaacctcacg agatattgcc
     9901 ggggccgtgc cgcagtacta cttagaccac ggggccgggg gcacgggtaa agcgcagtct
     9961 gcgacgtcgc catcctgatc agcgccgagg gtgcatgaga ccagccgggc gaatctcgca
    10021 gcgaacactt ccggaatgac atgaaataga aaccgccgta gagagattgg acgagtgaca
    10081 ggggaagtat gagcggctcg aagcgcgagg tcattggagt cgtcggcggc atggggccgt
    10141 tggcccgggt ccgcctggtc gagctcatta ctcggctgac acccgcttca cgtgaccagg
    10201 atcatcgcga cgtggtgctc gtttccatgc ctggccgcat cagcgaccgc acggaattcc
    10261 tgcttggaca cagcgacgag aacccggcgg ccgctgtcgc agagagcgtg cagctgctcg
    10321 cgagcgccgg cgccacgtgc gtcggcgtgg cctgcagctc ggtgcatgcc cctccgattt
    10381 ggtgccatca ccgacattct tgctcgccga gaatgcgacg ttgagttgct gcacctcatc
    10441 gaggagaccg gtcgcgaagt cggcgcccgt ttgccacccg gctcgaaggt gggcgtgctc
    10501 ggcacgaccg gcaccatccg tagcgacgtg tacgggcggg tgatgcgcgc tgccggcttt
    10561 gaggtgctct acctggacga ctctcgacaa agtgaagtac atgcggccat atacaacgag
    10621 gtggacggcc tcaagtcagg cacgatcgtg agcgcgcgcg tgcgcgatct agtgacacag
    10681 gcagcgcggg agctcatcga cagaggtgcg cggtgcgtcg tcttgggctg cacggagctg
    10741 cccgaagcat tgcccgtgga gagcttgggc ggtgtgccgg tgatcgatcc atcggtggtg
    10801 atggccagcc gtctgatact caggactgca gacgccccgg gcacagccag ctagcctgcg
    10861 gcgctcggtc gggactgcag cgtcggcgcg gcggcggcca gtatcgcctc tcgatttccg
    10921 ttgagcggcg gatagattcg ctgcgcattg atcgtgcgct tggtggcctt ggcttcatca
    10981 ccggtggcaa cgacctggcg atcccagcct tcggtgtaca cagcacccgc aggacccagg
    11041 tccaaaaccg tcacatacca agggattcgg agtgaaagca tcggctcgcc cagcaggtcg
    11101 ctgatgacgt tgtaaccggc gaagcggccc atagggcggc cgtgctgaca cgacatcacc
    11161 gatacgtgcg tgtcgtccat catcgcggcc gcgacatcgc cggcggcgaa tacgtccggt
    11221 acaccgttga cacgcagata ctcatcgacg ggcagtcggc ctagccgatc aagtgccaca
    11281 cccaaatccg cggtcagcgc attggcgcgc atgcccgcac accacaccac cgtcgacgcc
    11341 gcaagcgttt cgccggtcga caatgtcacc cggtcggcct caatgacggt gacgcccact
    11401 ccaagtcggg tctgcacccc ggtgctattc agcccggcct cgatcactgg ccgcgccgac
    11461 gcgcccatgt ccgagcccac gtgcgggttg tggtcaacga gcacgacccg gccgccggca
    11521 tcggggaaca gtgcggtcaa ccgcgcaggc agctcgcagg cggtctcgat gccagtcagc
    11581 ccggcgccca ccacaacgac ggtgctcgcg gcgggcgtgt gcggacgacc ggcgagtgcc
    11641 tccaggtgcc gttgcagcgc catcgcgccg ccataagtgt cgacgtcgaa accgaactcg
    11701 cgcagccccg gaatgtcggg tttcaccacc cggctgccca gcgcgaccac gagacggtcg
    11761 taaacgtgtg tgccgacaga acttttcacg gtgcgcgaga tcgcgtcgat cgccgttacg
    11821 tcggcggtta tgtgcgcaac cccggccggg tcgagcacgt cgcccaatgg gatgcggcac
    11881 ggggtcaggt cggcttcgta attgcggacc cgaatgtcgt ggtagggctg cgagctgagc
    11941 acggtgatgt gcacgtcgcc gttggcggaa ccgagttcgt caagccgccg ggccgcaccg
    12001 agcgctgccc acagccccgc gaaacccgaa ccgagcacga ggacttcgct catccggctg
    12061 acgctaccgc tcgcggatag agcagctcca gttcgccgag attggccgcc acggtggtca
    12121 gcggcagcgc ggcggagatc gccagatcgt cgttggttgc gtcggcgcgc agcgagagca
    12181 cgaagtcgtc gctgatcgtg tggagctccc ggccgggatc gccggtcagc cgagcctcga
    12241 tcgcatcggc gtgcgattcg agtacctcgc gggtccgctc gtacaccccc agcggcggag
    12301 gcacgacgtc ggcgatcaac tcggcggccg cgcgcaaccg gatggcgcgg ttggcggccc
    12361 gtaccaccgg tgcgcgcaac tctgtcatgc cgccgttctc cgaaaggtat tgccggacag
    12421 catcatccac tgtccgcgag gcttccagcc ccgcgcggct gagtgcgatc acgcggtcgg
    12481 tggcatcctc ggaggcgcca cgcgtcaccc gcagcaccgc ctgcgtcaag aactcggcgc
    12541 cgacggcgcg ggcatcgtcg atcgccttgg acaccgcagc cgtcgcgccg cgcggccaca
    12601 gcaggaccga caccacgatg ccgaccagcg cgccgaccac gacgtcctcc actcggatca
    12661 atcccacctg ccagcccgtc ggcacgatca ggttgaaatt gatcaacacc atcatcgtga
    12721 atgcggcctg gccggcgacg aacgacgcca cctcgggcac gtacgccgac ccgaacgcca
    12781 cgatcggcaa caggatccac agcacaacgg gatccacgcc gagcagctcg atcacgatcg
    12841 cgcccaatac gaaaccgacc gcggtgcctg cgaccgctcg cagcacgcgc gtgcccgttg
    12901 tcagcgcgct gctgcgcaac accgacattg cgccgagcac cacccagaat ccgtgctcga
    12961 ctgggaacac atgagtgacc gcgaccgcga cggccaaccc gagccccgtc cgaatgctgt
    13021 tgcgcagcac gaccgcccgg gtggcaagga atcccttggt gatggcggcg acggcgacgg
    13081 tttctggcat gacccaatcg gcggcgccgg tatcgggcag ccgacggccc agcacccgtg
    13141 cccacaccgg ccgggcgtcg gccgcggccg cgttgcggat gatgcggccg gtcaccgcga
    13201 tggtggccgc gatggtgcga cggttcaaca gcgtgcgacc gatctccacc gccgccccat
    13261 cgctggattc accgagtaat tcggcgatgt cctcgcggta gcggccctgc gacaccgacc
    13321 gcagctccgt caatgcatcg cggaggtcgg cgctgcgctc cgcccgcccg gcaacgcttg
    13381 gtaaccgcag caccgccgcc gagtcgcgca acacccgcac cgccgggtcc ttcatcggcc
    13441 ccagcatccg accggtctcg tcggtgatcc gatcggacag ccagccgagg tcgtcaacca
    13501 cccgaaccag tgcacggctg cccgcggtca acgcgaccgg ccggtagtca gcgccaagga
    13561 aggtcacgta gagcgcattc atcgccttgg tgacatcgcg tgccgacacg gtgccctcca
    13621 gtcggccggc caggcggtta cacacgcgtg ccgcgtttgc gcgcagctcg tcgtgatggc
    13681 gcggcggaaa gaggaacagc gccgcaggga cgcacaacgc cagcgcgatc agccaaccca
    13741 gcagccgctc cggaaggggc ccgaccggaa tgcaggcggg gagcacgaac atcagtagcg
    13801 tggcgcgctg accggcggca acgatctcac tgagcacccc tgcgaagatc accgccacac
    13861 cgagaacgaa catcaccgcg acgctcaccc agggatgcgg cgcgaccagc gtgcccagcg
    13921 tgatgaggat ggcgccgttg atcccgaggc cgctatacgc cagggcgcgc gcaggccggt
    13981 tacccgggaa gtcgaccagt atcagcagcg ccaccgatcc gaagatggtg aagagcggcg
    14041 tctgcgagcc gccgccgacc gtgaagctga ccgccgccgc gatgggcaac acgatcgccg
    14101 cacgcgacgc gcgccgcagc gcgtcgaatt cgggatcgcg tgcacgccac cggtatccca
    14161 cgtgtcgcaa caccgcggcc gtcccggccg cgctactcac cgtctggcac cgacgcgaac
    14221 cggccggagt caagggcgtc gagcagttgg ccggcgatcc attccagcgc atcgctgtcg
    14281 cgtggcaggg tggcctgttc gtagccgacc aacagataca ccccccacgc cgcgaacagc
    14341 tgggcttgcc tcttgttctg cagaatctcg aaggccgact cgtacatgat gtcgaagcgc
    14401 tgttgatcca ccgccgcctg gacggggtac acctgcgggt cgaccgaact ccacacccgg
    14461 atcgcggcct cggcgccgtg cgggagcgcc agcccgacgt tgatcaacgt gtcgatgcgt
    14521 cgccgcggat ccggctcgcc gcggaccgcc tcgatcacct gcacggtgcg gccttgcatc
    14581 cagtgcgcga ccagttcctt ggtgtaggcg ggccagccgg agaagtagtg atagaaggag
    14641 ccggtggtga cgccgagacg gttgcagacc tcggccagct tcaatccgcc gtatcccagg
    14701 tctgacaaaa cgtcgaggcc ggtttcgaaa taggcctccc tcgatgcgac gctcgccatg
    14761 gcagcaccct agttcgatga cgcccactct tccgagggcg atcggcaagc ccgacggacg
    14821 gtgggggccc ccgccgggcc ctgagaattt gctggatcga cccgtcgatg gggcgaggtt
    14881 tcgacaccgg gtcagacagg gcagtgacgt gtggcacaat ttgaccgtgc cgcaacatac
    14941 ggcgagtcga ggacccggtc gcccacccgc agcgaaggca gcagaaacgc gtgagcgcat
    15001 cgtacaagcg gctcgagaag tcttcagcga actcggctac gacgccgcca ccttccaggc
    15061 gatcgcgatc cgcgccgacc tcacccgtcc cgccattaac cattacttcg cgagcaaacg
    15121 ggtgctctgg cgcgaagtcg tcgagcaaac caacgccctg atagttagcg cagggatgga
    15181 acgggcccaa aacgaaacca atttgttggc tcggctgtcg gcgttcttct ccgtcgcgat
    15241 gcaggcggac tccgaagatc gttctgcggc agcatttctc gttacctcgg tactcgagtc
    15301 gcaacggcat cccgaattgg ccagcgacga ccacgactca ctgaccgcgt cccgcgaatt
    15361 catgtcgtgg gcggtcaacg acgcaatcaa actcggcgaa ctcaccaccg acaccgacgt
    15421 caaccatctg gtcgaaatgc tggtcgcggt gatgtggggc atgggcttct atgccggctt
    15481 cgtcggaagc cacgacgagc tgaattcggt ggtgcacaag ttcgagctgt tgatggccaa
    15541 caagctgtgg aacctgcacg aataaacccg caacctgcgg tgatgtagcc cacagtctgt
    15601 atagctctgc taagttttta gatagcattg ctgaatgacc gatgtgagtg aggtgcggct
    15661 ggtccgcacg gatggcgaca gctgggacat caccgaaagc gtgggcgcga cggccttggg
    15721 tgtcgccgct gcgcgggccg cggaaaccgc tcgcccggac gcgctgatcc gcgacgaatt
    15781 cgcctatctg ttgaccgcct ccgcaggccc cgcgtgggcg cagatggcca gtgcagacga
    15841 cgagtggctc ggtgacaacg aggatgcccg gcggatgcag gccatggcgc gtgactacca
    15901 ggccgtgcgg acgcactact tcgacggcta cttcaccgcg gcggttcgca ccggtatccg
    15961 ccaggtcgtc atcctggccg cgggactgga ctcccgcgcg tatcgactcg actggcccgc
    16021 gggcaccacg gtgttcgaga tcgaccagcc gaaggtgctc gagtacaaga cgtcgacgct
    16081 ggacgcccac ggtgcagtgc ccacctcgcg acgcgtcccg gtggcggtgg acctgcgcga
    16141 cgactggccc gcagcacttg tgtccgccgg gttcgatccg gcgcaaccga ccgcctggct
    16201 cgccgaaggc ctgctgccgt atctccccgg cgacgcccag gaccggctct tcgagatgat
    16261 caccgcgaac agcgcggcgg gcagccagat cgcggtcgag gcgttcaaca ttcaaccgtc
    16321 gcagtactcc ccggaaaagc gagcggcccg gcgggaacga tccgcccagc tgcgcgagcg
    16381 cctgggactg gatgtcgacg tcgacacatt gatgtacacc gacgacgcgc gggccgacgc
    16441 cgccgaatgg ctggccgcgc acggctggcg cgtcgccgcc gtgcccagcg ccgaggagat
    16501 gacgcgcctg gggcggccga ccaccgaaaa cctcgtggag gaagcgctgg acagcgtgtt
    16561 cgtgcacgcc cggctagagg ggtagctcgc gctgaggttg ggccttctgg gcgacccaac
    16621 cggttggtta ggatcgggta ttcctcctcg ggtcaggagg gacacccacc atgaccaccg
    16681 aatccgtcgc gcctgaagcc gtcaaagctc cggccaccga catccccgcc atcgtcggca
    16741 aacttcggga cacattcaag tccggtcgca cccgcagcgt tgagtggcgc aaacagcagc
    16801 tgcgggctct cgaggcgatg atgacggaga acgaggcggc catcgccgcg gcgctcgaac
    16861 aggacctggg ccgcagcccg ttcgaggcat ggctggccga catcgccagc accgcaggcg
    16921 aggccaagga cgccgcaaag aacgtgcgca agtggatgcg ccgcaagtac cgcctgctgg
    16981 agatgtcgca gctgcccggg cgcggatgga tcgagtacga gccctacggc accgtgctgg
    17041 tcatcggcgc ctggaatttc ccattcgtct tgacgctggg acctgctgtc ggcgcgatcg
    17101 ccgcgggcaa caccatggtt ctcaagccct cggaggtgtg cccggcatcg tcggcgctga
    17161 tggccgagct ggtgccgaag tacctcgaca acgacgcgat cgtcgtgatc gaaggcgacg
    17221 gagcggtcag ccaggagctc atcgcgcagg gcttcgacca catctgcttc acgggtggca
    17281 ccgagatcgg ccgcaaggta tatgaaggcg ccgcacctca tttgacgccg gtcaccctgg
    17341 agctcggcgg caagagcccg gtgatcgtgt ccgcggacgc cgacatcgac gtggcggcca
    17401 agcggatcgc ctggaccaag ttcatcaact ccggccagat ctgcatcgcg ccggactacg
    17461 tgctggccga cgcgaagatc cgcgaccaac tcgtcgacaa aatcaaagct gccgtgacga
    17521 ccttcgaggc ggaaaacccc ggcggcaggc gcatcgtgaa ccagcggcac ttcgaccggc
    17581 tcacggcgtc gctggccgcg accaagggcg atgtcgtcat cggcggcggt tcggattcgt
    17641 cgaagatcag catccagccg acggtcgtcg tggaccccga cccggccgaa ccgctgatga
    17701 ccgacgagat cttcggaccg attcttcccg tggtcaccgt ccaatccctg gacgacgcaa
    17761 tcggtttcgt gaactcgcga ccgaagcccc tttcggccta tctgttcacc aagaccaaga
    17821 gcattcgcga tcgggtgatc aaagaggtgt cggccggcgg catggtgatc aaccacctgc
    17881 tgttccagtt ctccacgaac aagctgccgt tcggcggcgt cgggccgtcg ggcatgggcg
    17941 cctatcacgg caagttcggc ttcgagcagt tcagccacaa gaagaccgtg atgaccaagc
    18001 caacccgacc cgatgtcggc gccttcatct atcccccgta tacagagaag gctttcaagc
    18061 tcgccagacg gctgttctag gaaggaacct tcatgccagg agtgcaggat cgtgtcatcg
    18121 tcgtcaccgg ggccggaggc ggactcggac gcgaatatgc gttgacgctc gccaaggagg
    18181 gcgccagcgt agtcgtcaat gacctcggcg gtgcgcgtga cggcaccggt gagggacatt
    18241 cgatggccga tcaggtcgtc aaggagatca aggacgcagg cggccgcgcg gtggccaact
    18301 atgactccgt ggctgagccg gagggcgccg cgaacatcat caagactgcc gtcgacgagt
    18361 tcggcaaggt cgacggtgtg gtttccaacg cgggcatcct gcgcgacggc accttccaca
    18421 agatggagtt cgctaactgg gacgcggtgc tcaaggtgca cctgtacggc ggctacaacg
    18481 tgatccgcgc ggcgtggccg cacttccggg agcagagcta cggccgcgtc gtcgtcgcca
    18541 cctcgaccag cggcctgttc ggcaacttcg gccaggccaa ctacagcgcc gccaagctcg
    18601 ggctcgtcgg cctgatcaac acgctggcgc aggagggcgc gaagtacaac atcaagacca
    18661 atgccgtcgc gccgatcgct gccacccgca tgaccgagga cattcttccg aaggaagtgc
    18721 tggagaagct gacgccggaa tacgtggcgc cggtcgtcgc gtacctgtgc accgaagagg
    18781 tgcccgagac cgcgtcgatc ttcatcgtgg gtggcggcaa ggtgcagcgc gccgcgctgt
    18841 tccagaccga cggcgtcacg ttcccggatg tcccgagcgt cgacgacgtc gcgggcaagt
    18901 ggggcgagat caccgacctg tcggcggcgc agcctgccac gttcagcctc cgctgagatc
    18961 tagatgaaag cgcttgtagc gcaggagctt tccggacctt cgggactggt ctacaccgac
    19021 gtcgaggatg tcggcggcga ggacgcaatt gtcgtcgacg tcggcgcggc gggggtgagc
    19081 tttcctgacc tgttgctcct gcgcggcgag tatcagctgc gtctggagcc gccgttcatc
    19141 cccggcatgg aggtcgccgg ggtggtgcgg tcggcgccct acgagtccgc gttcaagccg
    19201 ggccaacggg tgaccgcgct cgcgatgctg ggtgggtggg ccgaacaagt ggtggtgccg
    19261 gcggagaata ttcggcccac tccggatgac ctcgacgacg ccgaggccgt cgcgctgctg
    19321 ggcaattacc agacgatgta cttcgcgctg gccaaacgcg gcgcgctacg gcacggcgag
    19381 acagtgttgg tgctgggatc ggcgggcggt gtcggcacgg ctgccgtgca gatcgcgaag
    19441 gcgttgggcg ccaaggtaat cgcgatggtg caccggccgc acgcaacaga gtttgtcgag
    19501 tcgttgggcg ccgacgtcgt gctgccgttg accgagggct ggctgcaggc ggtgaaggac
    19561 gcgacaagtg gccgcggggt ggacctggtg gtgg
//
LOCUS       DASRJA010000806         6586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Mycobacterium sp. isolate SMAG_U10124 k141_3522619,
            whole genome shotgun sequence.
ACCESSION   DASRJA010000806 DASRJA010000000
VERSION     DASRJA010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963652
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Mycobacterium sp. (soil metagenome)
  ORGANISM  Mycobacterium sp.
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:13:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,278
            CDSs (total)                      :: 7,237
            Genes (coding)                    :: 7,079
            CDSs (with protein)               :: 7,079
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 63 of 158
            Pseudo Genes (incomplete)         :: 112 of 158
            Pseudo Genes (internal stop)      :: 14 of 158
            Pseudo Genes (multiple problems)  :: 28 of 158
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Mycobacterium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3522619"
                     /isolate="SMAG_U10124"
                     /isolation_source="soil"
                     /db_xref="taxon:1785"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VFC01_36350"
     CDS             <1..920
                     /locus_tag="VFC01_36350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZN85131.1"
                     /translation="GEWSGSVLLTMEWGDQKRRGLLGSFAQVGVPVGLVLGTGGMTLL
                     SATMSDEAFDSWGWRIPFLASLILVAVGLVIRLKILETPMFSKVLEQGKAASAPVAEV
                     IRHHWREILLSAGLRFVEQMPFYLFTTFVLTYVVQRHHYSKTFVLTAVLVGATVELAT
                     IPFFSHLSDSLGRKRVYLAGAITAGLFAFPYFAILTHGGQVPIFIAVVLSMIVHSMMY
                     GPQAALIGESFPTNLRYGGAGLGYQLASVFAGGPAPLLATWLLHETGTPYSISVYIVI
                     AAVITVVCCLALPDRSRADIDDASVYSRV"
     gene            complement(917..1876)
                     /locus_tag="VFC01_36355"
     CDS             complement(917..1876)
                     /locus_tag="VFC01_36355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZN85132.1"
                     /translation="MSAGEQRAGVDLTNLDQPLSPDAGATKRDLVNYLDAIADRMLPG
                     LVGRPLTVLRVLRGQAPFMQKNAPKYTPDWVKTVPIWAEASKREVHYPLCDDRRTLMW
                     LANQRAVEYHPSLGLADNIYRPTHLILDLDPPADDFDAVVAVAFVVRQALADSGLAGA
                     VKTSGAKGVHVFVPIDDSAPVDDVAAATRALAARAEALDPALATTAFIVEDRGGKVFV
                     DSTRAGGATVVAAYSPRLRPGTPVSFPLDWSDLDQISPADFTLHTAVDALAGRDPWAE
                     NMPAAQRLPSDLIEHGRAIPVARVAAMHEGKRRARARRADGHR"
     gene            1970..3328
                     /locus_tag="VFC01_36360"
     CDS             1970..3328
                     /locus_tag="VFC01_36360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014710792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HZN85133.1"
                     /translation="MEPISPTDALFLIGESREHPMHVGSLQLFQPPDGAGPDFVRDSY
                     NAMLACTDVQPMYRKRPAFFGGVTNLAWSFDKDVELDYHLRRSGLPQPGQVRDLLELV
                     SRLHGSLLDRHRPLWEAHLIEGLQDGRYAVYTKFHHALMDGVSALRLMQRAYTPDPSD
                     DEVRVPWSLGRRPRREPTHRPSLLAQLGGAAGSVAALAPSTLKLARAALLEQQLTLPF
                     RAPRTMFNVRIGGARRVAAQSWSMERIRAVKSAAGVTVNDVVLAMSAGALRAYLLEQD
                     ALPDDPLIAMVPVNLRKQDDDRGGNLVGTFLCNLATDVEDATERLEIISASMHGTKDV
                     FKQLPQVQQLALSAFNIGGLAFGLIPGYLSTAAPPFNIVISNVSAGRAEPRYWRGARL
                     DGNYPLSIALDGQAVNITVTNNADNLDFGLVGCRRSVPHLQRLLGHLETSLKDLERAT
                     GA"
     gene            3395..3892
                     /locus_tag="VFC01_36365"
     CDS             3395..3892
                     /locus_tag="VFC01_36365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85134.1"
                     /translation="MSSITFAGPRLTAAAAAACLVLFAIGASSASAGPAADDQGYVDS
                     TARCTAPSTAVLFGSTETSRVAICKTADGQYEYRGVRLSDGAKLIVPAADSGNGFVAE
                     NGEISYLVTAKSLVVSVGHNVIRDEPMVDFHQPDGPAAPPPSTTTPTTPLPPPLPAEV
                     GGSGR"
     gene            complement(3889..4272)
                     /locus_tag="VFC01_36370"
     CDS             complement(3889..4272)
                     /locus_tag="VFC01_36370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019736441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZN85135.1"
                     /translation="MSGLLLDTHVLLWLLDDSPRLGVNARQRITANAAVYVSAASTWE
                     LAIKAAIGKLNLPDELDGAIERSALRDLPVTRRHTLASDLTALPHKDPFDALLVAQAK
                     VERLTLLTADKKLLMALPDAVDAKL"
     gene            complement(4269..4499)
                     /locus_tag="VFC01_36375"
     CDS             complement(4269..4499)
                     /locus_tag="VFC01_36375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013827119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HZN85136.1"
                     /translation="MTTVNIHDAKTHLSQLLARVENGETITIARAGKPVADIVPHVRT
                     DIVFGELSGRLDYDADHFDDADNDLNALFGIG"
     gene            4797..6008
                     /locus_tag="VFC01_36380"
     CDS             4797..6008
                     /locus_tag="VFC01_36380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZN85137.1"
                     /translation="MSFSLELSEDVIQVRDWVHEFAATVVRPAAAEWDEREETPWPII
                     QEAAKVGLYSMELFATQAAEPTGIGMLTVFEELFWGDAGIALSILGTGLAAASLAANG
                     TPEQMGEWLPQMFGTPEDPLLASFCSSEPGAGSDVSAILTRARYDEASDEWVLNGVKT
                     WATNGGIANVHIVVASVHPELGSRGQATFIIPPNTSGFKQGQKFKKHGIRASHTAEVI
                     LEDVRLPGGLIVGGKDKFDERVARIREGQSAAGQAAMKTFERTRPTVGAMALGVARAA
                     YEYALEYAQQREQFGRKIADFQAIAFKLADMKARVDAARLLVWRAGWMARNNKPFTNA
                     EGSMAKLVASETAVYVTDEAIQILGGNGYTREYPVERMHRDAKIFTIFEGTSEIQRLV
                     MGRAITGLPIR"
     gene            complement(6012..6362)
                     /locus_tag="VFC01_36385"
     CDS             complement(6012..6362)
                     /locus_tag="VFC01_36385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZN85138.1"
                     /translation="MTAIDRRDTNEVGRAGRITNWVLALATIPGAVAVVLFLYMQIMG
                     TAGCNDQPCPRQGPGELGFVLIQYGAPIVAVVAVVLSFFTARRRGGILVPVVAWLLLI
                     AAFVVLVFSFTPTG"
     gene            complement(6392..>6586)
                     /locus_tag="VFC01_36390"
     CDS             complement(6392..>6586)
                     /locus_tag="VFC01_36390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HZN85139.1"
                     /translation="VAYYTVIAGSSVLVPILAYAIAGERVDHQLERLKQWMERQHAVL
                     MAGFLAVVGLLLVYTGIRAV"
BASE COUNT         1169 a         2130 c         2127 g         1160 t
ORIGIN      
        1 gcggtgagtg gagcggttcg gtgctgctca cgatggaatg gggcgaccag aagaggcgtg
       61 gactgctcgg cagcttcgcc caggtcggcg tccccgtcgg actggtgctg ggcactggcg
      121 gaatgaccct gctctccgcg acgatgtcgg acgaggcgtt cgactcttgg ggctggcgga
      181 tacccttcct ggccagcctc atcctggtcg cggtgggcct ggtgatccgg ctgaagatcc
      241 tcgagacgcc gatgttcagc aaggtgctgg agcaaggcaa agccgcgagt gccccggtcg
      301 ccgaagtgat ccggcatcac tggcgcgaga tcctgctttc cgcaggtctg cgcttcgtcg
      361 agcagatgcc gttctacctt ttcaccacgt ttgtcttgac ctacgtcgtg cagcgccacc
      421 actacagcaa gacgttcgtg ctgaccgcgg tcctggtggg cgccaccgtc gagctcgcga
      481 cgatcccgtt cttctcacac ctgtcggatt cgctcggcag aaagcgggtt tacctggccg
      541 gagcgatcac cgcaggcctg ttcgcattcc cgtacttcgc catcctgacg cacggcgggc
      601 aagtcccgat cttcatcgcg gtcgtgctgt cgatgatcgt gcactcgatg atgtacggac
      661 cgcaggccgc gctgatcggc gagagcttcc caacaaacct gcggtacggc ggcgccggcc
      721 tcggctacca attggcttcg gtgttcgcgg ggggaccggc tccgctgttg gcgacgtggc
      781 tgctccacga gaccggaact ccgtactcaa tctcggttta catcgtcatc gccgcggtca
      841 tcaccgtggt gtgttgcctt gccctgcctg accgttcgcg tgccgacatc gacgacgcgt
      901 ccgtctacag ccgcgtttaa cggtggccgt cggcccggcg cgcccgagcc cgacgtttgc
      961 cctcgtgcat ggctgccact cgggcgaccg gaattgcgcg cccgtgctcg atgaggtcgc
     1021 tcggcagacg ctgtgccgcg ggcatgttct cggcccatgg gtcgcgcccg gcaagcgcgt
     1081 cgaccgctgt gtgcagcgtg aaatcggccg ggctgatttg gtcgaggtcg gaccaatcca
     1141 acggaaacga caccggtgtg cctggacgca ggcggggact ataggccgcg accacagtcg
     1201 cgcctcccgc ccgcgtcgaa tccacgaaca cttttcctcc gcgatcttcg acgatgaaag
     1261 ccgttgtcgc caaggcagga tcgagcgctt cagcacgggc ggccagcgca cgggtggccg
     1321 ccgccacatc gtcgaccggc gcgctgtcgt cgatcggcac gaacacatgc acgcccttcg
     1381 cgccgcttgt cttcaccgcg ccggccaacc cgctatccgc cagagcctgg cgcactacga
     1441 aggcaacggc caccactgcg tcgaagtcgt cggccggtgg gtcgaggtcg agtatcaagt
     1501 gcgtcggccg gtagatgttg tcggccaagc ccaatgaggg gtggtactcg accgcacgct
     1561 ggttggccag ccacatcaga gtgcgccgat cgtcacagag cggatagtgc acttctcgtt
     1621 tggaggcctc agcccagatc ggcaccgtct taacccagtc gggggtgtac ttcggcgcgt
     1681 tcttctgcat gaacggtgcc tgcccgcgaa gcacgcgcaa cacggtcagc ggccggccga
     1741 ccaggcccgg caacatccgg tccgcaattg cgtccagata attgaccaga tctcgcttgg
     1801 tcgcaccggc atccgggctc aggggctggt cgaggttggt cagatcgacc cctgcgcgct
     1861 gctcgccggc gctcatatcc cgaccctacg tgtgcggatc gccgactctg ggcacacgat
     1921 tactctgatg tgccacccgc aggcacaggc ggaagaaacg aggccaaccg tggagccgat
     1981 ctcacccacc gatgcgttgt tcctcatcgg ggagtcacgc gaacacccca tgcatgtggg
     2041 atcgctgcag ctgtttcagc cgcccgacgg cgcggggccg gacttcgtgc gcgactccta
     2101 caacgcaatg ctcgcctgca cagacgtcca accgatgtat cgcaaacgtc cggccttctt
     2161 cggcggagtc accaatctgg cttggtcgtt cgacaaggat gtcgagctgg actatcacct
     2221 gcggcgctca ggactaccgc agccggggca ggttcgtgat ctgctcgagc tggtctcccg
     2281 gttgcatggc agtctgctcg accggcaccg gccactgtgg gaagcgcacc tgatcgaggg
     2341 cctgcaggac ggccgatacg ccgtctatac gaagttccat cacgcgctga tggatggggt
     2401 gtcggcgctg cggctgatgc aacgggctta caccccagac ccatccgatg atgaggtccg
     2461 cgttccgtgg agtctcggtc gtcgtccgcg tcgagagccg acgcaccggc cgtcgttgtt
     2521 ggcgcagctt ggcggcgcag caggttcggt ggcggcgctc gctccgtcga cactcaagct
     2581 ggctcgcgcg gcgctgctcg agcaacagct gacgctgccg ttccgcgcgc cgcgcacgat
     2641 gttcaacgtg cgcatcggcg gtgctcgtcg agtggccgcg cagtcttggt ccatggagcg
     2701 gatcagagcc gtcaagtcgg cggccggcgt gaccgtcaat gacgtggttc tggccatgtc
     2761 ggcgggcgcg cttcgtgcct acctccttga gcaggacgcg ttgccggacg acccgctgat
     2821 cgcgatggtc ccagtgaacc tgcgcaagca ggacgacgac cgaggcggca acctggtcgg
     2881 cacgttcctc tgcaatctgg cgaccgacgt cgaggacgcg accgagcggc tcgagatcat
     2941 cagtgcctcg atgcacggca ccaaggatgt gttcaagcaa ctcccgcaag tgcagcaatt
     3001 ggcgttgtcc gcgttcaaca ttggtgggct cgcgttcggc ctgatccccg gctatctatc
     3061 caccgccgca ccgccgttca acatcgtcat ctccaacgtg tccgcgggtc gtgccgaacc
     3121 gcgctattgg cgcggtgccc gattggatgg caactacccg ctgtcgatcg cgctcgacgg
     3181 ccaggcggtc aacatcacgg tcaccaacaa cgccgacaat ctggacttcg gtctggtcgg
     3241 atgccgtcgc agcgtcccgc atctgcagcg gctgctcgga catctggaga catcgctgaa
     3301 agatctcgaa cgcgcgaccg gggcctaacg attgtgtaga ggctcggcct cgaccctcgc
     3361 gtaaataaac acgggcgtgt tctggtgaaa gctgatgtcg tccattacct ttgcgggccc
     3421 acgcctgacc gccgccgcgg cggcggcgtg tttggtgctg ttcgccatcg gggcgtcgtc
     3481 ggcttcggcc gggccggctg ccgatgacca gggctacgtc gactcaaccg cgcggtgcac
     3541 ggcgccgtcc acggcagtcc ttttcggcag cacggagacc tccagagtgg ctatctgcaa
     3601 gaccgcggac ggtcagtacg aataccgcgg cgtgcggcta agcgatggcg ccaagctgat
     3661 cgtgccggcc gcagactccg gtaacggatt cgtcgccgag aacggcgaga tcagctatct
     3721 ggtgacagcg aagtctctgg tagtcagcgt gggccacaac gtgattcgcg acgagccaat
     3781 ggtggacttc caccagccgg acgggccagc agcaccgccg ccgagcacaa caactccgac
     3841 gacgccgtta ccacctccgc tacctgccga ggtcggtggc agcggacgct agagcttggc
     3901 gtcgacggcg tcgggcagcg ccatcaggag cttcttgtct gccgttagca gcgtgagccg
     3961 ctccaccttc gcctgggcga caaggagtgc gtcgaagggg tccttgtgcg gtagtgcggt
     4021 gaggtcggac gcgagagtgt ggcgacgtgt gacgggcaga tcgcgcaggg cggatcgctc
     4081 gatcgcgccg tcgagttcgt caggcaggtt gagcttgccg atagccgcct taatagcgag
     4141 ctcccacgtg ctcgctgcgg agacgtagac ggctgcgttt gccgtgatcc gctgcctcgc
     4201 attgacgccc agccgcggac tgtcatctag cagccaaagc aggacatggg tgtccagtaa
     4261 gaggccgctc atccgatgcc gaacagggcg ttgaggtcgt tgtccgcatc gtcgaagtga
     4321 tcggcgtcgt agtcgagtcg gccggagagt tcgccgaaga cgatgtcggt gcgcacgtgc
     4381 gggacgatgt cggcgaccgg cttcccggcg cgggcgatcg tgatcgtctc gccgttctcg
     4441 acgcgagcga gcaattgcga caagtgggtc ttcgcgtcgt ggatgttgac cgtcgtcacg
     4501 aaaccagtct actagactta gtctagttgg actagggcgt ggcgcatgcc tgcgggccga
     4561 cccgggtcga gttcgcgtcg gcacacatcc cgcaccgcgt cctcctggcc gcagggcccg
     4621 tacgtcgaca ggagttcctt caacagctcg tggtggctca ccaccctgac ttacccacag
     4681 tgatagtgag gcactccaca ggccgcggac cttaccgagc ggtaagtatc agtgtacagt
     4741 tgttcaccga agcgcggcgt taccgcgctg gacaggcacc gaaaggacga gtgactgtgt
     4801 cgttttcact tgaattgtcc gaggacgtga tccaggtcag ggactgggtc cacgaattcg
     4861 ccgccaccgt ggtgcggccc gcggccgcgg agtgggacga gcgcgaggaa accccatggc
     4921 cgatcatcca ggaggcggcc aaggtcggcc tctactcgat ggagttgttc gccacccaag
     4981 ccgctgaacc aaccggtatc ggaatgctga ccgtgttcga ggagctgttc tggggcgacg
     5041 ccggcatcgc gctgtcgatc ctgggtaccg ggctggctgc cgcatccctt gccgccaacg
     5101 gcacacccga acagatgggg gaatggctcc cgcagatgtt cggtacgccc gaggatccgc
     5161 tgcttgcgtc gttctgttcg tcggaacccg gcgcgggctc tgacgtcagc gccatcctca
     5221 cccgcgcccg ctacgacgag gccagcgacg agtgggtact caacggcgtc aagacctggg
     5281 ccaccaacgg cggaattgcc aacgtgcaca tcgtcgttgc gtcggttcat cccgagctcg
     5341 gcagccgcgg gcaggcgacg ttcatcatcc cgccgaatac ctcaggcttc aaacagggac
     5401 agaagttcaa gaagcacgga atccgcgctt cgcacaccgc ggaagtgatc ctcgaggacg
     5461 tccggctccc gggcggcctg atcgtcggcg gtaaggacaa gttcgacgag cgagtcgccc
     5521 gcatccgcga aggccaaagt gccgcaggcc aagccgcgat gaagacgttc gaacggaccc
     5581 gacccaccgt cggcgcaatg gcgctcgggg tagcccgcgc tgcgtatgag tacgcattgg
     5641 agtacgcaca gcagcgcgaa cagttcggcc gcaagatcgc tgactttcaa gcgattgcgt
     5701 tcaagctggc cgatatgaag gcccgcgtcg atgccgcccg gctgctggtg tggcgcgctg
     5761 ggtggatggc gcgaaacaac aagccgttca cgaacgccga aggatcgatg gccaaactcg
     5821 tggccagcga aaccgcggtc tatgtgaccg atgaggccat ccaaatcctc ggcggcaacg
     5881 gctatacgcg tgaatacccg gtcgagcgca tgcatcgcga tgcgaagatc ttcacgatct
     5941 tcgagggcac cagcgagatc cagcggctcg tcatgggcag ggcgatcaca gggctgccca
     6001 tccggtagaa gctacccggt cggcgtaaac gaaaagacaa gcacgacaaa ggcggcgatc
     6061 aacaacagcc acgccacgac cggaaccaga atcccaccgc ggcgtcgcgc ggtgaagaac
     6121 gacagcacga ccgcgaccac cgcgacgatg ggcgccccat attggatgag gacgaagccc
     6181 agctcgccgg gaccctgtcg cggacaaggt tggtcgttgc atccggctgt gcccatgatc
     6241 tgcatgtaca gaaacaagac caccgcgacg gcgcccggaa tcgttgccaa ggccaggacc
     6301 cagttcgtga tgcgcccggc acgccccacc tcgttggtgt ctcgccggtc gattgcggtc
     6361 actgtcgccg cgtacccgac cgtgcggcgt ctcaaaccgc gcgaatgccc gtgtacacca
     6421 acaacaaacc gacgaccgcg aggaagccgg ccatcaaaac ggcgtgctgg cgttccatcc
     6481 actgcttcaa acgctcgagt tgatgatcca cgcgctcacc ggcgatggcg tacgcgagga
     6541 ttggcaccag gaccgacgac ccggcgatga cggtgtagta cgcgac
//
